LOCUS       DAOQDO010000001        16814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_1408_length_16814_cov_3.372158, whole genome shotgun sequence.
ACCESSION   DAOQDO010000001 DAOQDO010000000
VERSION     DAOQDO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_16814_cov_3.372158"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKZ53_00005"
     CDS             <1..236
                     /locus_tag="PKZ53_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ38842.1"
                     /translation="GVRRKAGIEDFHFHDFRHTAPTWLAEMGVDAFTIADILGHSTIQ
                     MSMRYTHATDTRKANAMHKLSQICHKPGDVKVG"
     gene            complement(260..336)
                     /locus_tag="PKZ53_00010"
     tRNA            complement(260..336)
                     /locus_tag="PKZ53_00010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Val,seq:cac)
     gene            complement(437..1108)
                     /locus_tag="PKZ53_00015"
     CDS             complement(437..1108)
                     /locus_tag="PKZ53_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ38843.1"
                     /translation="MYCPNCGTQPQLADQKFCKTCGTNLSMVSQVLSAPASGPGVPAP
                     YIPAVLPPAAMMLPNQVAMAPTTSPNFPQATVHQPISLTEIPSLPYMEQERVRQKFMK
                     IAWALTLGGPFLIPTLAILGEFLFDTGELALLGVPVFLFGIFLLLYTKIALRKPNLPQ
                     VIVIQQGNSTQEMTPSPVSAVPGAMNHQLPAGVSTPLYTPGMFTEHTTQHLQVPYSQP
                     KRNTK"
     gene            complement(1182..1799)
                     /locus_tag="PKZ53_00020"
     CDS             complement(1182..1799)
                     /locus_tag="PKZ53_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38844.1"
                     /translation="MVVSQVLRTTPPVPSVPVSPYAGMPGNAPGLPVYPSPPAAPQTN
                     PVGPIPLANLSSLPFVEEELKRQKLRKLGWMMIGGGLLFAIFLDTLGDAIAHIINPLG
                     RAISALDGIGALICLAGIFLVIYTRFILKPAQMPPVIVVQSPQMTGNLNPNQVSQAGP
                     GPVIAQPGQLPPPSVDSYFYQSPSVTEHTTQQLKVPYPSSPRDTQ"
     gene            2086..3507
                     /locus_tag="PKZ53_00025"
     CDS             2086..3507
                     /locus_tag="PKZ53_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ38845.1"
                     /translation="MFHLQKSLARLVLSMVVLFSGGIPASAQSVSQLEPPPSDPIPVT
                     QRELLLNDLPIVVAPRPGKSQVTITALVKIGATFDLAGKAGLAALTAQSLLAGSKNRD
                     GSPVTVIQLKDELESVGGQISIVTGWDDTRLTITGPARNATTFIEILGRLVTFPGFSE
                     DEFNKIKQAQIAMREASSLQDQTDARFAQDLYGKHPYHHSLEGTAESLRGISRFDVID
                     FYKRHYLSNNAALVVVGDVTMAQILRTASQSFGVWTKGKPAPYTFLPPNEFQGTKIVF
                     HPNPAQSQVQIRAGAFTGSTFNGLLNWKLIDKLLKTQWSAETALAETRHLPDSPWMVR
                     LTASSDQVPSTIDSFQKALKTLRETPIPAETLKVAKEAVLKDYQMNFQSNASIANLLI
                     DMERYQIGRRSLAEWPQQLQALSAADLQPYLERLGGKQLLVVVQGGPLELAEKLRSLG
                     TVEVVSPASTSPTKSPSPQPEAK"
     gene            3651..4850
                     /gene="lepB"
                     /locus_tag="PKZ53_00030"
     CDS             3651..4850
                     /gene="lepB"
                     /locus_tag="PKZ53_00030"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNJ38846.1"
                     /translation="MQDQELSLTWEPQAKNHPVEDPLPLVPTSEPVTSEPTGLEPQLN
                     SFVSPVVEVEARLIEIQPESEPVPAPAMAEALLIPGTEANESQPPDDRFQDEPSAKQA
                     IETESAMTESPLAAAVEEMSPPETSDEGTAHPVSKTIISEAPPPLNRLYRPIYTVDRL
                     HPQAGLLVEDDEQITVTIPNPAHSTEPAGLFDRMVTGVGLTTPDKARHWLREGILLGR
                     DLLFAIAAIVIIFCYVLQPVKVEGTSMLPHLHDGQRIFINKFIYKLEPIERGDIVVFW
                     YPMDPEKSFIKRVIGLPGDELKLLGGKLYINGKLVSEPYLSAEFTKNGIFNRTWEVEP
                     YHYFVMGDNRDASNDSRSWGSVPEMYIYGKAVYRYWPFDEAGKLLSDDEVHDLRDIPH
                     RLSTGLD"
     gene            complement(4905..5654)
                     /locus_tag="PKZ53_00035"
     CDS             complement(4905..5654)
                     /locus_tag="PKZ53_00035"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNJ38847.1"
                     /translation="MAELKIIEHTGYLVLRLTGNDGLHRLELKTLEALHHHTQLWESR
                     TDLAAIVITGVPARSFAVGASIKELQHLTPDNAPVYATLGQSLFRRMRESSRVYLAAI
                     DGFCLGGGLDLALACDIRLATPRSQFAHPGAKLGIITGFGGTSRLPQTIGETAASRIL
                     AAAETITGHTALAWGLVNRLYSSTEILDHACALANTIASGPPHLAAMAKELAYQVAHL
                     PVPQANVVEARTWDLWRAEADFSRRRKPGRS"
     gene            complement(5827..7377)
                     /locus_tag="PKZ53_00040"
     CDS             complement(5827..7377)
                     /locus_tag="PKZ53_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ38848.1"
                     /translation="MSSDRKILRQTLHGVLALILVFAGVVSYHMVTTRAGNGAHFKSS
                     SLFSVGAQSPSDLPPINSPTELSNVFRTVSRRLKPAVVSLNVTEKVDTASMFSFGKNH
                     PAIPGFPDQDDEGGQALRRGTGSGFIVSEDGYIITNNHVVGKAEKIEVRLHDGKTYTA
                     KLIGADPNPDVAVIKVNSKEPLPHVGLSSPEEEAAKVNSMEQGDWVIAIGSPFGLEQT
                     ITAGIVSATGRDLPQGSQYSRYIQTDASINPGNSGGPLINLRGDVIGMNTMIFTESGG
                     NQGVGFAIPVDLVTKIARKLMTTGRVQRGWLGVSLYPRVLTEDEAKAIGLPDADGALI
                     QDLVADNSPAAQAGLKSGDFIVEFNGKPIKSGRDLTSQVADAEVGRDVEVKFLREGKE
                     QSTKVKLAERPDDDQMARGITPDSPGKEAKLGVSSTPVTAEMAQQMKLRINSGALIQS
                     VTPGSPADEGGLQRGDVVHTINRQPVANTDDLIRIVKSLPAGSTAVLQVERVIGGRAT
                     MLFLTVNL"
     gene            7665..8039
                     /locus_tag="PKZ53_00045"
     CDS             7665..8039
                     /locus_tag="PKZ53_00045"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNJ38849.1"
                     /translation="MFEVIIEASFSAAHRLRGEENAEPLHGHNWRIEVHVRGPKLDDK
                     HMLVDFRDVRAATQKVMEYLDHKYLNELPPFANEMNPSTENIAAFVLHEVAAQINTDR
                     YKVCKVRAWETPNTSASYEVAY"
     gene            8127..8666
                     /locus_tag="PKZ53_00050"
     CDS             8127..8666
                     /locus_tag="PKZ53_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38850.1"
                     /translation="MRLRSSWAGGIFLLMTTIGIGLSQGSVQTGAAVCSQPDAICPTG
                     GGRFDPWDLAFPLPKNLKWQTTYYSAPFFAILLKSVKAEPDDGPGSDKPCAGYIKEDE
                     RQRVQRLFQDRKVFASRYGCSEFLTGYTNVNAEYNFLAVYGGKTQAEAEKILARVRAT
                     GQFPDANIRKMQAIYDNGD"
     gene            8737..10308
                     /locus_tag="PKZ53_00055"
     CDS             8737..10308
                     /locus_tag="PKZ53_00055"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNJ38851.1"
                     /translation="MHPVSQRTSAFLSPAPAVSLEKAFPTPFKNVIATARTCYSSKGI
                     IRDEDITSGFEPLAQSIYQAGHHTTYQHAHFQFAMVNISRQFIWSFLHSHPFYNSEQV
                     SQRYVAVKPGSFAIPALTGEALTRYTTAIDEQMAAYEHLTELLMPVVAREYNKLFTKH
                     NTADKKIQAKLRKRALEAARYVLPVATFAYMYHTVSGITLLRYYRLCEQFDAPTEQRE
                     VATAMVQALLAHDPLYKTVLEEPLPLQETPEFQFFEAHHTATQSASRQQFLTEFDQSL
                     EGYTAKLIDYKPNNESVLAASVREVLGLPASVLSDTDAIELVLSPTQNRLLGESLNLA
                     TMSKLGRTLVHPHYTFRKKLSHTADSQDQRHRMTPASRPVLAAHFTGEPDYITPELIK
                     QEAHIEQFYHDTMARSWERIHQLKACGVSDEFALYLLPNAVSVRFTESSDLANLHHKH
                     RMRLCYLAQEEIWRASVEEARQVRQVNPHIGSHLLPPCTQRLQARSKPICPEGDRFCG
                     VRVWKLDLDEYQRVF"
     gene            10424..11593
                     /locus_tag="PKZ53_00060"
     CDS             10424..11593
                     /locus_tag="PKZ53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNJ38852.1"
                     /translation="MQTGLERIFDHHLDWLRGKRVGLIVHPASVDLCYQHVADLFHAR
                     PEFEITALFGPQHGIRGETQDNMIEWEGWPKDSRIGAPVYSLYGKVRKPTPEMLENVD
                     VLVFDVQDVGTRVYTFIYTMALAMEAAKEKGIPFVVLDRPNPINGRDLEGPILEPAYA
                     SFVGMYPIPMRHGMTIGELARLFNHIGNIGCELYVSEMAGWNRTLWIDKTWQPWVMPS
                     PNMPTLDTATVYPGMVVFEGTNISEGRGTTRPFEIIGAPFIEPYQLANHLNSIGLAGA
                     YFRPLFFQPTFHKFVGEICGGIQIHIQNRASLRSFQIAVEILKALHHFYPEHFAWRQS
                     PYEYVFDKCAFDIIVGNNTLRQQIEANLPWEAISATWQDEVEAFIELRREFLLYE"
     gene            11772..12659
                     /locus_tag="PKZ53_00065"
     CDS             11772..12659
                     /locus_tag="PKZ53_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ38853.1"
                     /translation="MAFKDIIGKLRGWIDDEEDVEVSEKRRAPSRWEEFLVTVARGIE
                     EVMQAEMFTPPGEPTYIPPEYIVFLSKEDDADWQGEKRRGFERGLHTVIAERARELVG
                     SGRLQTQTIALELRVDPNLEKGKFRVQPVWDSTPEMTQVKPRTPPKPAPAPAPTSPTV
                     PKTDRLDEGATVVKPRGQTAGLIDDEATVVRPRAALFSITVLHAGQHQSSTPCYKSKV
                     GIGRGSQEDVRLTGDMEISRSHLELERTDDGRFYITCLGRNPVIIGGKEVQTDRRAEV
                     TPGEPIYLCSFTLQIDLPA"
     assembly_gap    12715..12814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12874..14784)
                     /locus_tag="PKZ53_00070"
     CDS             complement(12874..14784)
                     /locus_tag="PKZ53_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38854.1"
                     /translation="MSKSFLRWSLWGIVLVSLLALASDRGPLAAADKLPLARLMPRGA
                     LAYVQTKDLAGLLKTWRDSKTKENYFNSPSYRAFTQSRLYLELQERLREFEQGAGIEF
                     TEDQAIAMAGGQSAATLYDMGKVELVYISELPLAQATATMLFKQSPRFDSHRRGSFEY
                     FVQELATDGGRLRQGLCFSASDGKLIITTTEPLMQRTLDNLINRSATDATDHLAGSMS
                     PVLAVAQDFTPHDMTLWVDAPRLRDQRFFGTYWVQGKQANLSQVNTSLADLEFAPGGI
                     WERRWVVLTDEVAKQSAPQTGAVNLVSLLQFVPDEAQSVSASAIASAAQQTQLTTRLA
                     KLIFPPLRPPIEPSNLSFSPPGYSEESDSADANQLERYRFLDQRFDQDVDDPASTLTG
                     TKAKLTVPSFQQQLDSQFEKDLADILSRAKPTSFVQLGNVKPESSGGPFMQINQAIVV
                     ECGSSFDAPRFEAAALQVLGGRLVVPGTAASFQWKALANGVRVPPTDLVQQGGAYQQV
                     GNYVIFASRPEFCAAIAAQFQAKRQPALLPETKTADRFAVVRLGAGRNAYRRLMQQLE
                     FVPSDTATEMGDEETGDGASGPNPFFSSNLNSLINVAGYVEDITLESSQKDQALTEVI
                     RYRFSPTAPKAK"
     gene            complement(14849..15874)
                     /locus_tag="PKZ53_00075"
     CDS             complement(14849..15874)
                     /locus_tag="PKZ53_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ38855.1"
                     /translation="MEGLLLFFLAIIVVGILITLAKSVYIVPQMSVMIIERLGTFQKL
                     ADSGLHLVMPFIERPRGMYWAGFRPGVTLIDLREQFTDLPMQPVITRDNVTVGVDSVI
                     YWQVTDPVKAVYEVADLTGGIMQLTTTALRNVMGEMDLDHTLSSRDTINNRLRMVMDD
                     ATHKWGVKVTRVEVRNINPPDDVRITMEKQMTAERNRRAVVLQAEGEKQAAITRAEGE
                     KQASITRAEGDKQMQILRAEGAAQARLRAVEAEAEALRQVASALGGQSNPAQYLIMGR
                     YIESLREMSAGQNSKVIFMPVETSQLFSSIGAFKELFQETQPQEGTAIPTQQILPPTR
                     TKQLVER"
     gene            complement(15938..16420)
                     /locus_tag="PKZ53_00080"
     CDS             complement(15938..16420)
                     /locus_tag="PKZ53_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNJ38856.1"
                     /translation="MEYLWVVWALLAVAFIVAEIFTTGFVLLWFGIGASLAALLALAG
                     VGGLGLQTLVFLFTSIILTAASRTIFLKYLVTDTDRSSLRVGTGSLPGQRGVVVTPSQ
                     GALREGEIKVYGSIWKAIPEEDIDLLQEGEEVEIARVDGNSLYVRKPRREPSWRELEK
                     "
BASE COUNT         4250 a         4429 c         4203 g         3832 t
ORIGIN      
        1 aaggtgtacg ccggaaggca ggaattgagg atttccattt ccacgatttc aggcacacgg
       61 cgccaacctg gttggcagag atgggagtgg atgcgtttac gattgcggat attcttgggc
      121 attccacaat tcagatgtca atgcggtata cacacgcaac agatacccga aaagccaatg
      181 ctatgcacaa attgtcacaa atttgtcaca agcccggaga tgtgaaggta gggtgaccag
      241 cgtaagttat tgagaggaat ggtgggcggt acaggactcg aacctgtgac ctctcgcgtg
      301 tgaagcgagc gctctaccaa ctgagactaa ccgcccacga gtgaaggacg cgcatagtac
      361 caaagcgact tcaggtcgtc aatcaaaaaa tcagtctgtc ccgactcgcc aacacaatcg
      421 gctggcttga ttccctctat ttggtatttc gtttgggttg agaatatggc acttgaagat
      481 gctgagtggt gtgttcagta aacatccctg gtgtatagag tggtgttgaa acacccgccg
      541 gcaattgatg attcatcgca ccaggaacag ccgatactgg agacggggtc atttcctgag
      601 tcgagtttcc ctgctgaatc acaatcactt gaggcaaatt gggcttcctc agcgcgatct
      661 tggtatacag cagcaaaaat atcccaaata aaaaaactgg aactcctaaa agcgccagtt
      721 caccggtatc aaacaggaac tcgcccagaa tggccagtgt tggaatcaaa aaaggaccgc
      781 caagtgtcag agcccaggca atcttcatga acttttggcg gactcgctct tgctccatat
      841 aaggaagcga tggaatttca gttaatgaaa tcggttgatg gaccgtggcc tgtggaaaat
      901 tgggggaggt tgtcggagcc atggcgactt gattgggaag catcatcgcg gctggcggta
      961 gcaccgctgg tatataaggg gcgggaacac ctggaccgga agctggagca ctgagcacct
     1021 gagaaaccat actcaggttg gtcccacagg ttttgcaaaa cttttgatca gcaagctgag
     1081 gctgggttcc acaattcggg cagtacataa aaaacctctg attccatcaa gtaatccgca
     1141 cacattcgca cttgaaaatt tgatctggaa gtcgccacca cttactgagt atctcgtgga
     1201 gatgatggat acggcacctt caactgttgt gtggtatgct cggtgacgct tggcgactgg
     1261 taaaagtatg aatcaaccga tggcggcggg agttgtccag gttgagcaat cactggacct
     1321 gggccagcct gagaaacctg atttggattc aaatttccag tcatttgtgg tgattgaacc
     1381 acaataaccg gcggcatttg agccggcttc aagataaacc gggtatagat caccagaaaa
     1441 atcccggcca gacaaatcaa cgccccaatt ccatccagag ctgaaatagc ccgccccagc
     1501 gggttaatga tgtgggcaat ggcatcaccc agggtatcaa gaaagatagc aaacaacaaa
     1561 ccaccaccaa tcatcatcca gccaagtttc cgcaattttt gacgtttcaa ctcttcttca
     1621 acaaagggca atgaggagag gttggcaagt ggaatcggcc caactgggtt ggtttgtggt
     1681 gccgctggcg gtgatggata aacaggcaaa cccggtgcat tccctggcat cccggcatag
     1741 ggtgagactg gtaccgaagg caccggtggt gtcgttcgga gcacctgtga aacaaccatc
     1801 aagtttgtcc cacaaatctt gcaaaacttt tgatctgcaa gctgcggttc ggttccacag
     1861 tttgggcaaa acatagctta aactcacaca gaccagtctc ttcaaacatt gaaggtggtg
     1921 actgaagagg aaatttgttg agagagaaaa gagagcgaag aacgtctgcg cagagtatca
     1981 taaaacatga gttgacgctg ctcttttttc atcgttagac tttgcaggat caggccatat
     2041 gcaggccgga aatcaacccc gccctaaacg ttaaggagtt atagattgtt tcacttacag
     2101 aagtctctcg cacgtctggt tttgagcatg gtggtgctgt tcagcggtgg aatccccgcg
     2161 tcggcccaat cggtctccca actagaaccc cctccttcag acccaattcc agtgacccag
     2221 cgtgagttat tgttaaatga cttaccgatt gtcgtggcac cccgccctgg caaaagtcag
     2281 gtcacaatta cagctttggt caaaataggg gccacctttg acctggctgg gaaagccggg
     2341 ttggcggcac tgacagctca aagcttgctg gctggctcca aaaaccggga tggatccccg
     2401 gtaacggtca ttcagctcaa ggatgagctt gaaagtgttg gtggtcagat atctattgtc
     2461 acgggttggg atgacactcg attgacaatc acgggcccag cccgcaacgc gaccaccttc
     2521 atcgaaattc tgggccgtct ggtcacgttc cccggctttt cagaagatga attcaataaa
     2581 atcaaacagg ctcaaatagc aatgcgcgaa gcctcatctc ttcaggatca aactgatgct
     2641 cgctttgccc aggacctgta tggcaaacat ccctatcatc attcgctcga aggcacggct
     2701 gagagtttgc gcgggatctc ccggtttgac gtgattgatt tctataaacg gcactatttg
     2761 tcaaacaatg cagcgctggt ggttgtcggc gacgtcacga tggctcaaat tctacgcact
     2821 gccagccaga gctttggagt ctggacaaag gggaaaccag ccccctatac cttcctgcct
     2881 ccaaatgaat ttcaaggcac aaaaattgtc tttcatccaa atccggctca atcgcaggtt
     2941 caaattcggg ccggtgcctt taccggatca acatttaatg gtttgctgaa ctggaagctg
     3001 attgacaagc tcctgaaaac ccaatggtca gcagagacag ccctggctga aacccgtcat
     3061 ctgccggata gcccctggat ggttcgattg acagcctcat cagaccaggt accttcgaca
     3121 attgactcgt tccaaaaagc actgaaaacg ctccgggaaa caccgattcc agccgaaacc
     3181 ctcaaggttg ccaaagaagc tgttctcaaa gactatcaga tgaatttcca aagcaatgcg
     3241 agcattgcca accttctaat tgatatggag cgctatcaga ttggtcgccg gtcactggcc
     3301 gaatggccac aacaactcca ggcactgtca gccgctgacc tccagcccta tttggaacgc
     3361 cttggcggaa aacaactgct tgtggttgta cagggaggcc ccctcgagtt agccgaaaaa
     3421 ctccgatccc tcggcactgt cgaggttgtg tcacccgcat caacctcacc gaccaaatca
     3481 ccatccccac aacctgaagc aaagtaaaca ttttttgcca ggatcaagta tagtgggcat
     3541 ctcaaccatg cacctttccc ggcacaccat accagcttaa actttgacga agcgaggacc
     3601 gatcaagaag attgatgttt gaaaaccata tcttcgagtc ataaccaact atgcaagacc
     3661 aggaactgtc acttacgtgg gaacctcaag ccaaaaatca cccagttgaa gacccactcc
     3721 cactggtacc aaccagcgag cctgtcacca gtgaaccaac tggtttagaa cctcagttga
     3781 attcctttgt atcaccagtt gttgaggttg aagccagact cattgaaatt cagcctgaaa
     3841 gtgagccggt tccagcaccc gcaatggcag aggccctgct gatacccgga actgaagcaa
     3901 atgagtcaca gccgcctgat gatcggtttc aagatgaacc atcggccaaa caagctattg
     3961 aaactgaatc agcaatgact gagtcacctc tcgcagcagc ggttgaagaa atgtcccctc
     4021 cggaaacttc agatgaaggg acggctcatc ctgtctctaa gacaatcatc tctgaagccc
     4081 ccccgccact taaccggctc taccgcccaa tttatacggt tgatcggttg cacccgcagg
     4141 caggcttact ggttgaagat gatgagcaaa tcacagttac gattcccaac ccagcccatt
     4201 caactgaacc agccggactc tttgaccgga tggtcaccgg agttggactg acaacccccg
     4261 ataaggcccg acactggctt cgagaaggta ttctcctggg tcgtgacctg ctctttgcga
     4321 ttgccgctat cgtgatcatt ttctgctatg tgcttcagcc cgtgaaggtt gaaggcacca
     4381 gcatgctgcc ccacctgcat gatggccaac gcatttttat caataaattt atctataagc
     4441 tggaaccaat tgaacgtgga gatattgtgg tgttctggta cccgatggac ccagaaaagt
     4501 catttatcaa gcgtgtgatt ggattgcctg gggatgaact caaattgctg ggtgggaagc
     4561 tctatatcaa tggcaaactg gtctcagaac cctatctttc ggctgaattt accaaaaacg
     4621 gaattttcaa ccggacctgg gaagttgagc catatcacta ctttgtgatg ggtgataacc
     4681 gtgatgccag caatgatagc cggtcctggg ggtcggtccc agaaatgtat atctacggca
     4741 aagccgtcta tcgctactgg ccgtttgatg aagctggcaa gctcctcagt gatgacgaag
     4801 tccacgatct acgtgatatt ccgcaccgat tgtcaaccgg gcttgattaa aaccagattc
     4861 tcgtcagttt tgacttttcc aaaaacaaac cgggccaaag taaatcaact tcggcccggt
     4921 ttccgtcttc ggctaaagtc tgcctctgcc cgccagagat cccaggttcg agcttcaacc
     4981 acatttgcct gaggtacagg caggtgtgca acctggtaag ccaactcctt ggccatcgcc
     5041 gccaaatggg gcggcccaga agcaatcgtg ttagccaggg cacaggcatg gtcaagaatt
     5101 tctgttgatg aatacagccg attgaccagc ccccacgcca aagcggtgtg gccggtaatc
     5161 gtttcagccg ctgccagaat acgactggct gccgtttccc caattgtttg tggcaacctc
     5221 gatgttccgc caaatccggt gatgatgccc agcttggcgc caggatgggc aaattgtgat
     5281 cgaggggttg ccagacgaat atcacacgcc agtgcaagat ccaacccgcc tcccagacaa
     5341 aacccgtcaa tcgccgcaag gtacaccctg gacgactcgc gcatccggcg aaaaagcgat
     5401 tgccccaacg tggcataaac cggcgcattg tcaggtgtta agtgctgtaa ttctttgata
     5461 ctggccccaa cggcaaatga ccgggcaggt acacctgtga tgacaatagc cgccagatca
     5521 gttcgtgact cccaaagctg ggtgtgatga tggagtgctt ccagggtttt aagctccagg
     5581 cggtgtagcc catcattccc agtcaaacgc agtaccagat atccagtgtg ttcaatgatt
     5641 ttgagttcag ccatagaatg ggcttggggt tgaagaagtt gggctgaaga ctcgcacgct
     5701 cggggctgaa gatttcgggc ctggggttga agaagtcggg cttggggctg aagaaaaggg
     5761 gttaaatgcc ctgagcccgg tttcttcagc ccggtttctt cagcccggtt tcttcagccc
     5821 gatgcgttag aggttcactg tcaaaaacag catcgtggcg cgtccgccaa tcacccgctc
     5881 aacctggagc acggcggtac tcccagccgg caaactcttc acaatgcgga tcagatcgtc
     5941 agtgttggcc actggctggc ggttgatggt gtgcaccaca tctccgcgct gtaatccacc
     6001 ttcatcagcc ggactaccag gagtaaccga ttgaatcaag gcaccgctgt taatccgcaa
     6061 cttcatttgt tgcgccattt cagcggtgac cggagttgag gaaacaccaa gtttggcttc
     6121 cttccctggg gaatccggtg tgatcccacg cgccatctgg tcatcatccg gacgttcagc
     6181 cagtttgacc ttggttgatt gttctttccc ctcacgcagg aacttgacct caacgtcacg
     6241 accaacttcg gcatcagcga cctgactggt taaatcacgc ccagacttaa ttggctttcc
     6301 attaaactca acaataaaat cgcctgattt gagaccagct tgagccgcag gtgaattatc
     6361 ggccaccaga tcctgaatta aggctccgtc tgcatcgggt aatccaatcg ctttcgcttc
     6421 atcctctgtc aggactcgcg gatagagact gaccccaagc cagccgcgct ggacccgccc
     6481 cgtggtcatc agtttccggg caattttggt caccaaatca accgggatgg caaatccaac
     6541 accttgattg ccacctgatt cggtaaaaat catcgtgttc atgccaatca catcgcctcg
     6601 caaattgatc aatggtcctc ctgagtttcc aggattgatc gaggcatcgg tttgaatgta
     6661 gcggctatat tgtgaacctt gaggcagatc acgtcccgtg gcgctgacaa ttccagccgt
     6721 aatggtctgc tccaaaccaa atggagaccc aatcgcaatc acccaatcgc cctgttccat
     6781 gctattgact ttggcggctt cttcttcggg cgaactcaag ccaacgtgcg gtaagggctc
     6841 ttttgaattg actttgataa cagccacatc ggggtttgga tcagccccga tgagctttgc
     6901 cgtataggtc ttgccatcat ggagccgaac ttcaattttt tcagctttgc cgaccacgtg
     6961 gttgttggta atgatatatc cgtcttcact cacgataaaa ccagaaccag ttccccgacg
     7021 caaagcctgg ccaccttcat catcctgatc aggaaagcct gggattgctg gatggttttt
     7081 cccaaagcta aacattgagg ccgtatcaac tttctctgtc acattgagac tgacgaccgc
     7141 tggttttaag cggcgactga ccgttcgaaa gacatttgaa agttcggttg gggagttgat
     7201 gggcggaagg tcagagggag attgagcccc aactgaaaaa aggctgctgc tcttgaaatg
     7261 ggctccattt ccagcccggg tggtcaccat gtgatagctc accactccgg caaaaaccag
     7321 aatgagagcc agaacgccat gaagggtttg tcgaagtatt ttgcgatcag aactcatagc
     7381 tatttgtttt ctcctcgaag ttggatagtc ctgcaacacc aagcctcaag gctcgtgatg
     7441 ggtcaatgtt caattggaat gaatgtgaaa caagtcagac gtgaagcgtt gccaaagaca
     7501 acggccataa cgatttcaag ccaccagggt tcgccgggaa aaccgcagtg gatagacttt
     7561 ccagtgaatt ttcattattg tacccgtttc tgagttcaat caagctcaca ttccatccca
     7621 atcacccagc cgcactgcat ttttctgcgg aatcataagc acctatgttt gaagtaatta
     7681 tcgaggcttc tttttcagcc gcgcaccgat tgcgtggtga ggaaaatgcc gaaccgctcc
     7741 acggtcataa ctggcgcatt gaagttcacg tgcgtggtcc aaaacttgac gataaacaca
     7801 tgctggtgga ctttcgtgat gtccgggccg ccacgcaaaa agtcatggag tacctggatc
     7861 acaagtacct gaatgagtta ccgccgtttg ccaacgaaat gaacccatcc accgaaaaca
     7921 ttgcggcgtt tgtgttgcac gaagtagcgg cccaaatcaa tactgaccgc tataaagtct
     7981 gtaaagttcg ggcctgggaa acgccaaaca cgtcggcatc ctacgaagtg gcttattgac
     8041 ctccgacaca ataccagcaa ccacctgttc attccgtcac tgttacctca aaagcatttc
     8101 ccacccatca acttcgggag gcaccgatga gactccgatc atcctgggca ggcggaattt
     8161 ttttgctgat gaccaccatc gggattggat tgtcgcaagg aagcgttcaa accggtgcgg
     8221 cagtctgcag ccaaccggat gcaatctgtc caacgggagg cggaaggttc gacccgtggg
     8281 atctggcgtt cccgcttccg aaaaacctga agtggcaaac gacttactat tcagcacctt
     8341 tttttgcaat tttgctcaag agtgtcaaag ctgagcctga tgacggccct ggaagcgata
     8401 aaccctgtgc tggctatatc aaagaagacg aacgtcaacg ggtacagcgg ctctttcagg
     8461 atcgcaaagt ctttgcttct cgctatgggt gttctgagtt tctcaccggt tacacaaacg
     8521 tgaacgctga gtacaacttt ctggcagtgt atggagggaa aactcaggct gaagctgaaa
     8581 agatactggc tcgtgtcaga gcaacgggtc agtttccaga cgccaacatc cgtaaaatgc
     8641 aggctatcta tgacaatggc gattgatcca gatgaatagg caaaagcggt gtcaagccac
     8701 cgcactccat gcaggaagca cctccactca gtttttatgc acccagtttc ccaacgcacc
     8761 tcggcttttc tctcaccggc tccggcggtc agccttgaaa aagcattccc gacaccattc
     8821 aaaaacgtga ttgccacggc gcggacctgc tattcaagca aagggattat ccgggacgaa
     8881 gacattactt caggatttga gccactcgcc caaagcatct atcaggctgg ccaccacacg
     8941 acctatcaac atgctcattt tcagtttgcg atggtcaata tttcgcgcca gttcatctgg
     9001 agctttttgc actcgcatcc tttctacaac agcgaacagg tcagccagcg gtatgtggcc
     9061 gtcaaacctg gatcatttgc cattccggcg ctgacaggtg aagcactcac ccggtacacc
     9121 acggcgattg acgaacaaat ggccgcctat gaacacctga ccgaactcct gatgccggtg
     9181 gtggcccgcg agtacaacaa gctcttcacc aaacacaaca cggctgacaa aaaaatccag
     9241 gccaagctcc gcaaacgcgc gttggaagcc gcccgatatg tgcttccagt ggcaacgttt
     9301 gcttacatgt accacaccgt cagcggcatc accctgctgc gctattatcg gctctgcgag
     9361 caatttgacg ccccgaccga acaacgcgaa gtcgccacgg cgatggttca ggcgctcctg
     9421 gcccacgatc cgctctataa aaccgtgctc gaagaaccgc ttccactgca agaaacacct
     9481 gaatttcaat tcttcgaggc ccaccacacc gccactcaat cggcatcccg ccagcaattt
     9541 ctgaccgaat ttgaccagag cctggaaggg tacacagcca aactcattga ttacaagccc
     9601 aataatgaga gtgtattagc cgcatcggtc cgtgaagtac ttggccttcc agcttcagtt
     9661 ctcagtgata ccgacgcaat tgaactggtg ctgagcccaa ctcaaaatcg cttactgggc
     9721 gaatcgctca atctggccac aatgtcgaaa cttggtcgga cgctggttca tccgcattac
     9781 acattccgca aaaaattgag ccacacggcg gattctcagg atcaacgcca ccggatgacc
     9841 ccagcttcac gaccagtcct ggcagcacac ttcacaggtg aacctgacta catcaccccg
     9901 gaattgatca aacaagaagc ccatatcgaa cagttttacc acgacaccat ggcacgctcg
     9961 tgggaacgca ttcaccagct taaagcttgc ggcgtctcag acgaattcgc cctgtatcta
    10021 cttcccaatg cagtttcggt gcggttcacg gaatcttctg acctggccaa cctccatcac
    10081 aagcaccgga tgcgcctgtg ctatctggct caggaagaaa tctggcgcgc atcagttgaa
    10141 gaagcccggc aggtccggca ggtcaatcca catatcggct cgcacctgct gcctccctgc
    10201 acccagcgac ttcaggcccg ctcgaaacca atctgcccgg aaggtgatcg cttttgtggc
    10261 gtgcgggtgt ggaaactgga ccttgatgaa taccagcggg tgttttaaca gggtttgggg
    10321 attggaaaac tgggtctggg aaaacaacta aagggacaaa aaggacaaaa aggacctaaa
    10381 ggacctaaaa atcgaagact ccgaaacctg aaccctaact cttatgcaaa ctggacttga
    10441 acgaattttt gaccatcacc tggactggct gcgcggcaaa cgggttggat tgattgtcca
    10501 tccggcgtcg gttgatctgt gctaccagca cgtggcggac ctctttcacg cccgcccaga
    10561 atttgaaatt acggcgctgt ttggccccca acacggcatt cgtggtgaaa ctcaggacaa
    10621 catgattgag tgggaaggct ggccgaaaga cagccggatt ggtgcaccgg tctatagcct
    10681 gtatgggaaa gtgcgtaaac caacgccgga aatgcttgag aatgtggatg ttctggtgtt
    10741 tgacgttcag gatgtcggta cgcgggtcta cacgtttatc tacaccatgg cactggctat
    10801 ggaagcggcg aaggaaaaag gaattccctt tgtcgtgctt gaccgcccca acccaatcaa
    10861 tgggcgtgat ctcgaaggac cgattctgga gccagcctac gcctcgtttg ttgggatgta
    10921 tccgattccg atgcgccatg ggatgacgat tggagaactg gcacgactct ttaaccatat
    10981 cggcaacatc gggtgtgaac tctatgtctc tgaaatggct ggttggaatc gaaccctctg
    11041 gattgataaa acctggcaac cctgggtcat gccatcacct aatatgccaa cccttgatac
    11101 agcaacggtt tacccaggca tggtggtgtt tgaaggcacc aatatttctg aaggtcgcgg
    11161 cacaacgcgc ccctttgaaa tcatcggggc accgtttatt gagccctatc aactggccaa
    11221 ccatttgaat tcaattgggt tggccggcgc atattttcgc ccgttgtttt ttcagccaac
    11281 gtttcacaaa tttgtgggtg aaatctgcgg tgggattcag attcacattc agaatcgggc
    11341 cagtttgcgg tcgtttcaga ttgcagttga aatcctcaaa gccctgcatc acttttatcc
    11401 agaacatttt gcgtggcgac agagccccta tgaatacgtt tttgacaaat gtgcctttga
    11461 catcatcgtg ggcaacaata cgctccgcca gcaaatcgaa gccaaccttc cctgggaagc
    11521 aatcagcgcg acgtggcagg atgaagtgga agcgtttatc gaactgcggc gggaatttct
    11581 tttgtatgag tagtttagtc aagggtcaat aatcaggagt caactcccaa ctatggaatg
    11641 atcgaatttt cttcaaagaa actacccata acctgcccgg atagaataaa atacacctgt
    11701 ctggcaatct cgattcccaa cactgagcgg ctgacgccta acgactgact tttgactcag
    11761 gcactaaacc tatggcattc aaagacatca ttggcaaatt gcgtggctgg attgacgatg
    11821 aagaagacgt cgaggtatcg gaaaaacgcc gggcaccaag ccggtgggaa gaattcctgg
    11881 taacggtggc gcgtggcatc gaagaagtca tgcaggctga gatgtttacg ccaccggggg
    11941 aaccaaccta cattccaccg gaatacatcg tgtttttgag taaggaagat gatgccgact
    12001 ggcaaggcga aaaacgccgg gggtttgaac gtggcctgca caccgtgatt gccgaacggg
    12061 ctcgcgaact ggtcggaagt ggtcggctcc agactcaaac tatcgcgctt gaactgcgag
    12121 ttgatccaaa tctggaaaag ggcaaatttc gcgtccagcc agtctgggat tcgactccgg
    12181 aaatgaccca ggtcaaaccc cggacaccgc cgaaaccagc accagcacca gcaccgactt
    12241 ccccaaccgt tcccaaaacc gacaggctgg atgaaggtgc cacggtggtc aaaccccgag
    12301 gccaaacggc tggcttgatt gatgatgaag ccacagtcgt ccgtcctcgg gcagcgcttt
    12361 tttcaatcac ggtgctccac gccgggcaac accagtcctc gaccccgtgc tataaatcta
    12421 aagtcggaat cggacgtgga tcacaggaag atgttcggtt gactggagat atggagatta
    12481 gccgctcaca cctggaatta gagcgcaccg acgatggcag gttttatatc acctgcttag
    12541 gccgcaaccc ggtgattatt ggcggaaaag aagttcaaac tgacaggcgg gctgaagtga
    12601 ctccgggtga accaatttac ctgtgtagct ttaccctgca gattgacctg ccagcttgaa
    12661 tgtggagtgc ggcggcttga cgccgcttta tttatgtgga gtgcggcggc ttgannnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcggcg gcttgacgcc gctttattta
    12841 tgtggagtgc ggcggcttga cgccgctttt tatttatttc gcttttggtg ccgttggtga
    12901 gaatcgatag cgaatcactt cagtcaacgc ctggtctttt tgactggatt ccagggtgat
    12961 gtcttccaca tatccagcta cattgatcag tgaattcaaa tttgaactga aaaacggatt
    13021 tggtccgctg gcgccgtccc cggtttcttc atctcccatt tcggttgccg tatctgatgg
    13081 cacaaactca agttgctgca tcaaacgacg ataggcgtta cgaccagcac caagtcgaac
    13141 aaccgcaaac cgatcagccg ttttggtctc cgggagcagt gctggctgac gcttcgcctg
    13201 gaattgagcg gcaattgcag cacaaaattc aggccggctg gcaaaaatca cataattccc
    13261 cacctgttga taagccccac cctgttgcac cagatcggtt ggaggcaccc gcacgccatt
    13321 ggccagcgcc ttccactgaa atgaggcggc agttccaggc acgaccagtc ggccacccag
    13381 aacttgaagg gcggcggctt caaaccgcgg agcgtcaaag gaactgccac actccaccac
    13441 aatcgcctga ttgatttgca taaagggccc accactgctt tccggcttta cattcccaag
    13501 ttggacaaaa ctggtcggtt tggcccggct caggatgtca gccagatctt tttcaaactg
    13561 gctgtcaagc tgctgctgaa aggaaggaac cgtcaatttg gctttggtgc cagtcaaggt
    13621 tgaagccgga tcatcaacgt cctgatcaaa tcgctggtcc aaaaatcggt accgttcaag
    13681 ctgattagca tcagcggaat ctgactcttc ggaataacct ggaggagaaa acgacagatt
    13741 cgacggttca atcggtggtc gaagcggcgg gaagatcaat ttcgccagac gtgtggtgag
    13801 ttgggtttgc tgggcggctg acgcaatggc tgaggcagag actgattgtg cttcatctgg
    13861 gacaaactgc agcaagctga ccaggttgac ggccccagtt tggggggcag attgtttcgc
    13921 aacttcatct gtcagtacca cccagcgccg ttcccagatg cctccagggg caaattcaag
    13981 atcggccaga ctggtgttga cctgactcaa gttggcttgt tttccctgaa cccagtaggt
    14041 accgaaaaac cgctggtcac gcaaacgcgg tgcatcaacc cagagcgtca tatcgtgtgg
    14101 cgtaaaatcc tgggcaaccg ccagcacggg cgacatactg ccggccagat gatcggtagc
    14161 atcagtggca ctgcggttga tcaaattatc cagtgtccgc tgcatcagtg gctcagtggt
    14221 ggtgatgatc aacttcccat cagaagctga gaaacacaat ccttgccgta accgtccacc
    14281 gtcggttgcc agttcctgga caaagtattc aaagctgcca cggcgatgtg aatcaaatcg
    14341 aggtgattgt ttaaagagca tcgttgccgt ggcctgggcc agtggcagtt cgctgatgta
    14401 aaccagctcg acctttccca tatcgtagag cgtagcggct gattgaccac ccgccattgc
    14461 aatggcttga tcttcagtaa actcaattcc agcgccctgt tcaaattctc gaagtcgttc
    14521 ctgaagttca agatagagcc gggactgggt aaacgcccga tagctgggtg agttgaaata
    14581 gttctctttg gtcttggagt ctcgccaggt tttgagcaat cccgccaagt cttttgtttg
    14641 aacgtaggcc agtgcgccac gtggcatcag tcgcgccaac ggaagcttat cggcggcagc
    14701 taacggccct cgatctgaag ccagcgccag caggctcacc agtacaattc cccacaacga
    14761 ccagcgaaga aatgatttcg acatatcaac accccacatt caacaaaaga gacacaccag
    14821 cagtgcccag tttaacccag gtcccaaatt accgctcaac cagttgcttg gtccgcgtcg
    14881 gaggcaaaat ttgctgggtt ggaatcgccg ttccttcttg cggctgagtt tcctgaaaca
    14941 gctccttaaa ggcaccgatt gaggaaaaca actgtgacgt ttcaaccggc ataaagatca
    15001 ctttgctgtt ttgaccggct gacatttcac gcaaagactc gatatagcgc cccataatca
    15061 gatattgcgc cgggtttgac tgaccgccca gggcacttgc gacctgacgc aacgcctcgg
    15121 cttcggcttc aaccgcacgc aaccgggcct gggccgcacc ttcagcccgc agaatctgca
    15181 tttgcttgtc gccttcagcc cgagtaatgg acgcctgttt ttcaccttca gcgcgggtaa
    15241 tcgccgcttg cttttcacct tcggcctgga gcacaaccgc acgtcggttt cgttcagccg
    15301 tcatttgttt ttccatggtg atgcggacgt catctggcgg attgatattg cggacttcca
    15361 cccgtgtcac tttgacaccc catttgtgag tggcatcgtc catcaccatg cggagccgat
    15421 tattgatggt gtcacgtgat gaaagggtat gatcgagatc catttctccc atcacattgc
    15481 gcaaggccgt ggtggtgagc tgcataatac cgccagtcaa atcagcgact tcatacacgg
    15541 ctttgaccgg atcggtcacc tgccagtaaa tcactgaatc aaccccaacg gtgacgttgt
    15601 cgcgagtgat gacgggttgc atcggcagat cagtgaattg ctcgcgcaaa tcaatcaatg
    15661 tcactccagg tcggaatccg gcccagtaca ttccccgagg ccgctcaata aacggcatga
    15721 ccagatgcag gccgctatca gccagttttt ggaatgttcc caatcgctca atgatcatca
    15781 cactcatctg agggacgata tagaccgact tggccagggt aatcaaaatc ccaaccacaa
    15841 taattgcaag gaaaaacaga agcagtcctt ccatgaaaaa tccccctttc agaatgggca
    15901 tgtcaacaag caacggcttg tctggggtga caggagttta tttttccaac tcgcgccacg
    15961 agggttcacg acggggtttg cgcacataca gactattgcc atcaacacgg gcaatttcaa
    16021 cctcttcgcc ttcctggagg agatcaatgt cctcttcagg aatcgccttc caaatcgagc
    16081 cgtagacctt gatttcccct tctctgagag caccctgact gggtgtaacc accactccgc
    16141 gctggccagg taaactccca gtgccaactc tgagcgaaga gcgatcagta tccgtaacca
    16201 gatatttcaa aaagatcgtg cgggaagccg ccgtcaagat aatggaagta aataagaaga
    16261 caagggtttg caatccgaga ccgccaactc cggccaatgc caggagcgcc gccagactcg
    16321 ccccaattcc aaaccagagc agcacaaacc ctgtggtaaa gatctcagcc acaataaagg
    16381 cgacggccag aagtgcccaa accacccata aatattccat aagcggatga tactgtgttg
    16441 ggttgaaatg atcctggttt gttgtcagtt gtcagttggt gataaaccca gattgttcaa
    16501 caggttgatt gactgtgaat cagagcaggt acgtctcaaa taagatgtgg tggcgatatt
    16561 gatattgcca gcttcattat gcctaccacc tttaagacac gtcaaaccta aagatgggct
    16621 gaagacattg ggcttggggt ggaagacttc gggctgaaga cattgggctt ggggtggaag
    16681 acttcgggct gaagaaagcg ggctgaagag tatggggtgg aagacttcgg gctgaagaaa
    16741 gcgggcttgg ggtggaagac ttcgggctga agaaaacggg ctgaagaaag cgggcttggg
    16801 gtggaagact tcgg
//
LOCUS       DAOQDO010000002        11489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_2706_length_11489_cov_2.914553, whole genome shotgun sequence.
ACCESSION   DAOQDO010000002 DAOQDO010000000
VERSION     DAOQDO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_11489_cov_2.914553"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..704)
                     /locus_tag="PKZ53_00085"
     CDS             complement(204..704)
                     /locus_tag="PKZ53_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ38857.1"
                     /translation="MNLTITEYTSNHQAAIRSILEGIGWAEQYIVAAEKNAEAFSQIP
                     DFFGVYLAMIEQTAIGFVYVQFYEWNRLAQLQGLAVDVRYQRQGIASQLVKRAEQFAQ
                     EKQARGIYVDTPTLNHKGRAFYEGIGYQFGYIMPRYYEDALDGVTYQKFFDGNATQPL
                     QAEDKR"
     gene            complement(818..1936)
                     /gene="mqnE"
                     /locus_tag="PKZ53_00090"
     CDS             complement(818..1936)
                     /gene="mqnE"
                     /locus_tag="PKZ53_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNJ38858.1"
                     /translation="MVNATLARIAEKVHTGERLTLEDGVALFETDQLAWLGQLADSVR
                     RKRHGQVVFYNVNRHLNPTNVCYWDCTFCSFYRKPNDPEAYTYTIEQAVREIRPSYEA
                     GITELHIVGGLHPTLSFEYYLELLRGLKAEFPGIHLKAFTMVELDHFRRITRLSAREV
                     IDRLVEAGLDSCPGGGAEIFAEPVRKQICKHKCGSQRWLDFSKEVHQAGLRSNATMLY
                     GHIESYADRVDHMIRLRALQDETGGFQCFIPLAFFPKDTELSHLPGPTPVDSLKTIAV
                     SRLMLDNFDHIKAYWVMLGRQTAQIALHFGANDMDGTVSGGGPLVATYSLDGAAQCQT
                     TKNEIVQLIQDAGLVPVERDTLYHPIQRNQPEAEKILA"
     gene            2363..4174
                     /locus_tag="PKZ53_00095"
     CDS             2363..4174
                     /locus_tag="PKZ53_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HNJ38859.1"
                     /translation="MCLTGVDYFSTLGYQPSIAFVAANVLSPIATLVLVALTLFGALP
                     IYSRVAAESPNGQGSISMLEDLLPRWRGKVFVLCLLGFAATDFVITITLSAADATAHL
                     LENPFAPHWIEGHQVAVTFVLLLMLGAIFLKGFKEAIGLAVFLVAGYLVLNVIVIVRG
                     CIEIVNHPHYISSWKLALSSGEYHGNPLVIIGMALLVFPKLALGLSGFETGVAVMPLV
                     EGDPSDTHDQPAGRIRNARHLLRTAALIMSVMLIGSSFVTTLLIPHEAFQTGGEASGR
                     ALAYLAHKYFGDGFGTAYDLSTMAILWFAGASALAGLLNLVPRYLPRFGMAPEWAKAN
                     RPLVVVFTVITLTVTWIFKADVDSQGGAYATGVLVLMTSAAVAVTLSLWKAGWRRYAF
                     LAITLVFAYTTIQNIHERPEGLKIASLFIISIVFTSLISRALRSTELRIHKVKFDETA
                     HRFLKEVARGTVRIVTNRPDQGTAEEYDKKEREARNTHHIPKGDPLLLLEVKISDASE
                     FTDDLLEVRGIQVGRHRVIRCESPAVPNAIAAILLHVRDTTGQIPHVYFGWTEGNPVV
                     YVLKYIALGEGDTAPVTREVLRQAITDPDDRPRVHVG"
     gene            complement(4335..5930)
                     /locus_tag="PKZ53_00100"
     CDS             complement(4335..5930)
                     /locus_tag="PKZ53_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ38860.1"
                     /translation="MFIPSRSWQQFEMVPSAFVFLQEESFDHRLIRFLIDGEGLLSNI
                     GVAVGVLLLLVLRFLLPPNRRTSLRFPLICLAIHVALVLAHMVFEPRPAFARLETILE
                     IAALFLLLTSLGRLGFLFFYEAVFVSQLSKTTPKIIGDILQGLVYVGVLLLTLKASGV
                     QLGSLLVTSALLTAVIGFALQETLGNLVAGLTLQIQEPFDLGDWIQLDGHVPLGEVTE
                     INWRATKMITLDRVEVTIPKGVLAKNSILNYTKPTNVARRNIYILVPYRIPPRKVHDI
                     ILKVLPEVSEVLTDPPASVVTNRILESGMVEYWVRFFITAFHRRDIIDGNVRDRIWYA
                     LERSEIDHPYGTSTIQLHRIDQQAHSYLREVEIQTIQRQLNSIPFLDPLNPEDLYELA
                     EKIQKRIYAPTETILRQGEPGEEFFIINAGQVSIQIDHREIKQIGVGAYFGEMSLLTG
                     EPRTATIVAISEVVVYVMGHAAFREILKKHPTLAEEMSRVLVSRQLEIEQLRVVSPET
                     EVEKQVIDRSRQLLAKIRQFFSL"
     gene            complement(6104..6580)
                     /locus_tag="PKZ53_00105"
     CDS             complement(6104..6580)
                     /locus_tag="PKZ53_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HNJ38861.1"
                     /translation="MTFRSIVLLLLLTGYEPVVYGWQSATPPAKTTAQETKTTREVRA
                     VLDAQVAAWNRGDLEGFMKGYWRSPELTFTSGTTQTSGWDETLARYKRRYQGEGREMG
                     TLTFADISILPLERHTTLARGRWKLVFSDGKVSEGLFTLVLKQLPEGWRVIHDHTS"
     gene            complement(6638..7603)
                     /locus_tag="PKZ53_00110"
     CDS             complement(6638..7603)
                     /locus_tag="PKZ53_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ38862.1"
                     /translation="MTNHVSPSHPLIEVHHLKKYFPAGQAQVKAVDDISFSIQTGETF
                     GLVGESGCGKTTTGRLMLRLIEPTAGEIRFAGENLLALSRKEMRARRRDLQIIFQDPY
                     ASLNPRMTVGEAIAEPLVIHQIGDAGLRRRRVAELLEVVGLSPDYASRYPHEFSGGQR
                     QRIGIARALALNPRFIVADEPVSALDVSVQAQVVNLLQDLQSQFQLTYLFISHGLAVV
                     EHLCTRVGVMYLGKLVEVASSLELYANPLHPYTQVLLSSIPIPDPALRRNRIPLTGDV
                     PTPINPPSGCRFHPRCPYAKEECRTTEPPLREIAPGHQAACWLLK"
     gene            7931..8599
                     /locus_tag="PKZ53_00115"
     CDS             7931..8599
                     /locus_tag="PKZ53_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ38863.1"
                     /translation="MMRIQDIAALSRSTDQIKSLSGGTAATETRLSVADAELLARLRT
                     GNREAFEELVTRYQSTIYNLAYRLLNDPEDARDVTQEIFLKIYQAASSFRGECEFRTW
                     IYRITVNQVANQQRWWRRRWRRETISLETTQTEDGQPLAERLACATDDPEQKTLLLEQ
                     RQQLTAALAAIKFDFRVAVILRDVKEMSYEEIADALQISVGTVKSRIARGREELRRRL
                     KHRF"
     gene            8647..9348
                     /locus_tag="PKZ53_00120"
     CDS             8647..9348
                     /locus_tag="PKZ53_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ38864.1"
                     /translation="MMDCTVCQEQCSAYLDLELEDGLRAEIEYHLEVCPVCQTYVHEL
                     QAVQTALVPLSSDVAIPDAWSRQWVAALEQASVPEHHSWWQPGAWTDWLQAAQPLLAG
                     YGVGIAVTCVLFSGILFSFKPIFSLGTPDYKMVYIQANTRPTMDPSSIGMANLPTQLA
                     AANQPMDCVIFIAEVSPQGKAQLVEMVSPTSDPEVVAQISDALGKSSFQPATYFGHPV
                     SSRVVLMIEQIYVRG"
     gene            9548..9985
                     /gene="rplM"
                     /locus_tag="PKZ53_00125"
     CDS             9548..9985
                     /gene="rplM"
                     /locus_tag="PKZ53_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNJ38865.1"
                     /translation="MSTYVPSGKNLEAERKWYVIDAAGLTVGRLASEVALLLMGKRNP
                     KYTPFLDLGDHVIIVNAEKVVFTGNKLNDKFYRHYTFHPGGLREISAKDQLQKHPERV
                     LTLAVKGMLPKTKLGRAMASKLKVCIGPTHPHQAQQPIPLEFK"
     gene            10040..10432
                     /gene="rpsI"
                     /locus_tag="PKZ53_00130"
     CDS             10040..10432
                     /gene="rpsI"
                     /locus_tag="PKZ53_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNJ38866.1"
                     /translation="MATTQYYGTGRRKTAVARVFLRPGDGKIVVNKRAFENYFPNEVH
                     RMVIRQPLHVTDTASKFDLYVTVRGGGISGQAGAVRHGITHALLALNAEFRRRLKKAG
                     FITRDPRGKERKKYGQKGARKRFQFSKR"
     gene            10632..11420
                     /gene="rpsB"
                     /locus_tag="PKZ53_00135"
     CDS             10632..11420
                     /gene="rpsB"
                     /locus_tag="PKZ53_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNJ38867.1"
                     /translation="MKELLEAGVHFGHQVRRWNPKMREYIFGERNGIYIIDLQKTQRL
                     LKESMRFVTQSAAEGKNFLFVGTKRQAQEAIAEEALRCNMFYVNHRWLGGFLTNFQTI
                     QKSIKRLKDLEAMRTDGRYEALTKKEIIKREKERETLERNLAGIKSMGRLPDVIFVID
                     TKKEEIAVAEANRLGIPVVAIVDTNCDPGGIDFVIPGNDDALRSVRLISSKIADAILE
                     GRQMGVEKAETPEVVPAAGGRRQADAVAVAAAPAPETTPQASAE"
BASE COUNT         2798 a         2875 c         3000 g         2816 t
ORIGIN      
        1 ctggggtatg ccgcaagtgt ttgctcaggt atttactgag tcgggtccgt tgtttttcgt
       61 ccatggtgtg tcctgattga aaacatcggg ctgaagaagt cgggcttggg gctgagggct
      121 gaagaactgg ttttagttct cctgaaccct tgtaattgcg ctgagcccga tgtcttcagc
      181 cccaagccct gagcccgaag tcttcagcgc ttgtcttcag cctgaagtgg ttgagtcgcg
      241 ttcccatcaa aaaacttctg atacgtgacg ccatccagcg catcttcata atatcgtggc
      301 atgatatacc caaattggta tccaatccct tcataaaaag cgcgcccctt gtggttcagg
      361 gtcggagtgt caacataaat tccacgggcc tgcttctctt gagcaaattg ttcggcacgt
      421 ttgacaagct gtgacgctat gccttgacgt tgatacctga catccaccgc cagtccctgg
      481 agctgggcga gccggttcca ttcatagaac tgaacataaa cgaacccaat ggcagtttgc
      541 tcaatcattg ccaggtaaac cccaaaaaaa tcaggtattt gggaaaaagc ttcagcattt
      601 ttttcagccg caacaatgta ctgctcggcc cagccgatcc cttcaaggat agagcggatg
      661 gctgcttgat gattggaggt gtactcagtg atggtcaggt tcataatcgt tgatgttctg
      721 cccaaggccc aaactaacag cctgcagaat gcttatatcc acaagattgt tttacatcag
      781 taatttagat tctactgtac cttcttattc accctggtca ggccaggatt ttctcggcct
      841 ccggttgatt gcgctgaatt gggtgataga gcgtgtcccg ctcaactgga accaggccgg
      901 catcttgaat caattgaaca atttcatttt tagtggtttg acattgggca gcgccatcga
      961 gtgaatacgt tgccacaagc ggaccgccac ctgagacggt tccatccata tcgttcgcac
     1021 caaaatggag tgcaatctgg gcggtttgac gacccagcat cacccagtag gctttgatgt
     1081 ggtcaaagtt atcgagcatt aaccgcgaga cggcaatggt cttcaaacta tcaaccggag
     1141 ttggtcccgg caggtgtgag agttcggtat ctttcgggaa aaatgccagc ggaatgaagc
     1201 actgaaaccc gccagtttca tcttgcagcg cccggagccg aatcatatgg tcaacccgat
     1261 ctgcatacga ttcgatatgg ccatagagca tcgtggcgtt ggatctcagt ccggcctgat
     1321 ggacttcttt agaaaaatca agccagcgct ggctcccgca tttgtgtttg caaatttgtt
     1381 ttcgaactgg ttcggcaaag atttcagcgc cgccacccgg gcaggaatcc agaccggctt
     1441 caaccaggcg atcaatcact tcacgcgccg agagtcgtgt gatccggcgg aagtgatcca
     1501 gttcgaccat ggtaaaggct tttaaatgaa tgccgggaaa ttcagccttg agcccacgaa
     1561 gtagctctaa atagtattca aatgaaaggg ttgggtgcag tccgccaaca atatgaagtt
     1621 ctgtaattcc tgcttcgtac gagggacgaa tttcccggac tgcctgctca atcgtgtagg
     1681 tataggcttc cgggtcgttc ggctttcggt aaaacgagca gaacgtacag tcccagtaac
     1741 agacattggt cgggtttaag tggcgattga cattataaaa caccacctgc ccatgtcgtt
     1801 tgcgtcggac agaatcagcc agttgtccta accaggcaag ctgatcagtt tcaaagagag
     1861 cgacaccatc ttcgagcgtc aggcgttcgc cggtgtgcac tttttccgca atgcgagcca
     1921 atgttgcatt caccataaag ccaaatttct cagtactctt tcaaaattag atgaattggg
     1981 gctgtcagga aggtttgaaa agaaccactt cagccaaaaa gaaaccgcat cttaatctga
     2041 tgtgaatcac cacagcaatc agaattcggc aagtctgttc ctggactttc aggtgtgaaa
     2101 attgctcgcc tgcttgacga aggcttcaca agtgcaatag gctccacctt ctgacgtcct
     2161 cttcagcctg agtccttgcc agttaaagtc cactttcatt tcgaagcaga gattctaaaa
     2221 cctcatggca cgaagcattt tcaaatcgaa gcaggcgcgc ctgtttcggc ggtggtttct
     2281 tgaaggcgcg cgtgatgttt catccaaacc acatcacgac ggggctcatc aacatcatcc
     2341 aacccatccc tggtggcagg tcatgtgttt gaccggtgtg gattattttt ccacccttgg
     2401 gtaccagccc agcattgcat ttgtggcagc caatgtgttg tccccaattg caaccctggt
     2461 tctggtcgca ctgaccctct tcggagcact tccaatttat agccgggtgg cggctgaaag
     2521 tcccaatgga caggggagta tttcaatgct ggaagacctc ctcccgcgat ggcgagggaa
     2581 ggtttttgtc ctgtgcttgc ttgggtttgc cgccactgat tttgtgatca cgattactct
     2641 ttcagcggct gatgccaccg ctcacctcct cgaaaatcca tttgccccac attggattga
     2701 aggtcatcag gttgcagtta cctttgtgtt gttgttgatg ctgggggcta tctttttgaa
     2761 agggtttaaa gaggctatcg ggttggccgt ctttctggtc gctgggtacc tggtcctgaa
     2821 tgtgatcgtg attgtgcgtg ggtgtattga gatcgttaac cacccccatt atatcagctc
     2881 ttggaaactt gctctttcaa gcggtgaata tcacgggaac cctctggtga tcattgggat
     2941 ggcgttactg gtatttccaa agctggcact tggcctgtca ggatttgaaa ctggtgtcgc
     3001 ggtgatgcca ctggttgagg gagatccttc agatacacac gaccagcccg ctggacgtat
     3061 tcgcaatgct cgccatttat tgcggaccgc cgcgctcatt atgagcgtga tgttgatcgg
     3121 cagcagtttt gtgaccacgc tgttgattcc ccacgaagcc ttccaaaccg gcggggaagc
     3181 cagcggacgg gcattagcct atctggccca taaatatttt ggtgatggat ttggcaccgc
     3241 ttatgacctg agcaccatgg ccattttatg gtttgctggg gcgtcagccc tggccggtct
     3301 gctcaacctg gtgccgcgct atctaccacg ctttggcatg gcgcccgagt gggccaaagc
     3361 caaccgtccc ctggttgtcg tctttaccgt cataaccctc accgtaacct ggattttcaa
     3421 ggctgacgtt gactcccagg gcggtgctta tgcaaccgga gtgctggttt tgatgacttc
     3481 cgccgccgtc gctgtaacgc tttcattgtg gaaggctggc tggcgacgat atgcgttttt
     3541 ggccatcaca ctggtctttg cctatacaac gattcaaaac attcatgagc gtccagaagg
     3601 gttgaagatt gcttcgttgt ttatcatttc cattgttttt acctcgctga tttcacgggc
     3661 tttgcgatca accgaactcc ggatccataa agtcaaattt gatgaaacag cccaccggtt
     3721 tttgaaagaa gtggccaggg gaacggttcg gattgtgaca aatcgcccag accagggaac
     3781 ggctgaggaa tatgacaaaa aggaacgcga ggcccgaaat acccaccaca ttcccaaagg
     3841 tgatccattg ttgttgcttg aagtaaaaat cagtgatgct tcggagttca ctgatgattt
     3901 gcttgaagtg agagggattc aggttggacg ccatcgcgtg attcgttgtg aaagcccagc
     3961 cgtccccaac gctattgccg cgatcttgct ccacgtgcgc gacacgaccg ggcaaatccc
     4021 ccacgtttat tttggctgga ccgaaggtaa cccggtggtc tatgtcttga aatatattgc
     4081 acttggggaa ggggataccg cgcctgtaac ccgtgaagtc ttgcggcagg ccattactga
     4141 tccagatgac cgtccgcgag ttcacgtcgg gtgatgaaaa gacattgggt tgaagaagtt
     4201 gggctgaaga actggctgta gttcatccct cattcttcat ccctttgatt gcccggagct
     4261 tgcgaatttt cagcaccaag ccctgagccc gttttctctt cagcccattg tcttcaaccc
     4321 tcaattcatt gttctcataa cgaaaagaat tgtctgattt tggccaggag ctggcgagat
     4381 cggtcaatga cctgtttttc aacctcagtc tcgggggaaa caacccggag ttgttcaatc
     4441 tcaagctgcc gactcaccag cacacgactc atctcttcag ccagtgttgg gtgctttttc
     4501 agaatttccc ggaaggcggc gtgccccatg acataaacca caacttcgct gatggcgaca
     4561 atcgtggcgg ttcggggttc accggtcagc agtgacattt ccccaaagta ggcaccaacc
     4621 ccaatctgtt taatttcccg atggtcaatt tgaatactga cctggccggc attgatgata
     4681 aagaattctt cacctggttc gccctggcgc aaaatcgttt cggttggggc atagatccgt
     4741 ttctggatct tttcggccag ttcgtacaga tcttcaggat tgagtgggtc aagaaacgga
     4801 attgaattta actgacgttg aatcgtttgg atttcgactt ctcgcagata cgagtgcgct
     4861 tgctgatcaa ttcggtgaag ctggatggtg ctggtgccat atgggtgatc aatttcagat
     4921 cgttccagtg cataccagat tcggtcgcgg acattgccat caatgatgtc gcgccggtga
     4981 aacgccgtga tgaaaaaacg cacccagtac tcaaccatgc ctgattccaa aatacgattg
     5041 gtcaccacgc tggctggggg atcggtcaaa acttcagaaa cttcgggcaa aaccttcaga
     5101 ataatatcgt gaactttgcg gggcggtatc cgatagggca ccagaatata gatattgcgg
     5161 cgtgccacat tggtcggttt ggtgtaattg agaatcgaat ttttagccag tacacccttt
     5221 ggaattgtga cttcgacccg gtcgagggta atcattttgg tggcacgcca gttgatctcg
     5281 gttacctcac ccagcggaac gtgcccatcg agttggatcc agtctcccag gtcaaatggt
     5341 tcctggattt gcagcgtgag tcctgcgacc agattgccca gtgtttcctg cagggcaaac
     5401 ccaataaccg cggtcaacaa ggctgaggtg accagcagtg agccaagctg gacccctgaa
     5461 gctttcagtg tcagaagcaa tacacccaca tagaccaatc cctgcaagat atcgccaatg
     5521 atctttgggg ttgtttttga cagttgtgag acaaataccg cctcatagaa aaataaaaag
     5581 ccaagccggc ccagggacgt aagcaataaa aagagagcgg caatttccaa aattgtttca
     5641 aggcgggcaa aggccggtcg tggttcaaac accatgtgtg ccagcaccag tgccacatga
     5701 attgccaggc atataagcgg aaaccgcaag ctggttcgtc tgttcggcgg gagcaaaaac
     5761 cgaagtacca gtaagagcag cacgccgact gccaccccga tattgctgag cagcccctca
     5821 ccatcaatca agaacctgat caaccggtgg tcaaatgatt cctcctggag aaaaacaaag
     5881 gctgatggaa ccatctcaaa ctgttgccaa ctgcgggatg gaataaacat gagttgtatc
     5941 ctgacctgaa atgctgaaaa ggaaggtatg ccctgtcatt caacctgaaa ggaaccactg
     6001 ttccttgaaa tcctggatcc gggaactcca gagtgttcaa tgacttgatt gttggcaaaa
     6061 acacactgat atttgaaaag ctgatgcctg tgagcctaat ggtttaagac gtatgatcat
     6121 ggatcacccg ccagccttcc gggagttgtt ttaaaaccag agtaaacaat ccttcagaga
     6181 cttttccgtc tgaaaacacc agtttccagc ggccacgagc cagtgtggtg tgccgctcaa
     6241 gtggaagaat cgaaatatcg gcaaacgtca gcgttcccat ttcgcggcct tccccctgat
     6301 agcgccgttt atagcgggcc agtgtttcat cccagccgct ggtttgggtg gtaccggacg
     6361 taaaggtgag ttccggagac cgccagtacc ctttcataaa cccttccaaa tcacctcggt
     6421 tccaggcggc cacctgggca tccaaaacag cgcgaacctc gcgggttgtt tttgtttctt
     6481 gagctgtcgt tttggcagga ggtgtcgctg attgccagcc gtaaactact ggctcatatc
     6541 ctgttaagag cagcagcaaa acaatagatc ggaatgtcat aagcgattgc ctccaggaaa
     6601 aaataagtgt gggaatgaga tgtagctcgg aataaggtta tttcagcagc cagcaagccg
     6661 cctgatgtcc aggtgcgatt tcacggagcg gcggctctgt ggttcgacat tcttctttgg
     6721 catagggaca acgcggatga aagcgacatc cagaaggggg attgatcggt gttgggacat
     6781 cacccgtcag cgggatgcga tttcggcgca aggccggatc cggaatggga atgctgctca
     6841 gaagtacctg ggtataggga tgaagcggat tggcgtagag ttccaggctg gatgccactt
     6901 caaccagttt gcccagatac atcacaccaa cccgcgtaca gagatgctca accactgcca
     6961 gtccatgtga aatgaacaaa taggtcagtt gaaactgtga ttgcagatcc tggagcaaat
     7021 tgacgacctg agcctgaacc gaaacgtcaa gggctgagac gggctcatcg gccacaataa
     7081 atctggggtt taacgccagt gcccgggcaa tgccaatccg ttgccgttgt ccgccggaaa
     7141 actcatgtgg gtaacgtgac gcataatccg gtgacaggcc gacaacttcg agtaattcgg
     7201 ctacgcggcg acggcgcaac cctgcatcgc caatctgatg aatgaccagt ggttcggcaa
     7261 tggcctcgcc aacggtcatc cgcggattga gcgaagcata tggatcctgg aaaatgatct
     7321 gtaagtcacg gcgtctggcg cgcatttcct ttcgagagag cgccaggaga ttctcacccg
     7381 caaatcggat ttccccagcc gtgggctcga tcaaccgcaa catcaatcgt ccagtagtgg
     7441 tctttccaca gccggattcc cccaccagac caaaggtttc acccgtttga attgaaaagc
     7501 tgatgtcatc cacggctttg acctgtgcct gaccagccgg gaaatacttt ttgagatggt
     7561 gtacttcaat cagaggatgt gaaggcgaaa catgattggt catgcgggtt gttccagaaa
     7621 ataaagagtg gagagccagc gagctgaaat ctgactcgca cagagaccat caacactggg
     7681 gaattatgtg cgccgaaaga agttcggacc ggaatatgga ccaccggctg tattcaggca
     7741 agctactctc gactctggat tgccgtcaac cagccttccg gtctggtcca gcaggttttt
     7801 agccagaaac agaaaaagtt tcactggatg tgggaacttt ccctgcaaga gggggtctaa
     7861 cgcgagtaac tcccaaacaa gcaggttgct ttttcagcag aatccaattt ttccgacaag
     7921 gtgcatgcgc atgatgcgaa tccaggatat tgccgcactg tcacgctcaa ctgaccagat
     7981 caaatcgttg tcgggtggga cggctgcaac tgaaactcgc ttgtcagtgg ccgatgctga
     8041 attgctggct cgtctgcgaa ccggcaaccg ggaggctttt gaagaactgg tcacgcgcta
     8101 tcagagtacc atctacaacc tggcgtatcg gttgttgaat gatcctgagg atgcacgtga
     8161 tgtaacccag gagatttttc tcaaaatcta tcaagcagcc agttcgtttc gcggtgaatg
     8221 cgaatttcgc acctggatct atcgcatcac ggtgaatcaa gtggccaatc aacaacgatg
     8281 gtggcgacgg cggtggcggc gggaaaccat ttcattggaa acaacccaaa cggaagatgg
     8341 acaaccgctg gccgaacgtc tggcctgtgc gacggatgat ccagaacaaa aaacgctgct
     8401 cctggaacag cgtcagcaac tcaccgctgc gctggcagcg atcaagtttg attttcgagt
     8461 cgctgtgatt ttgcgggatg tcaaggaaat gtcctatgag gaaatcgctg acgccctcca
     8521 aatctcagtc ggcaccgtca aatcacggat cgcccgtggg cgtgaagaac tccgacgccg
     8581 tctcaaacat cgcttttaag gaaggatatg attggaattc tttctggtga aaggagacag
     8641 ggcactatga tggattgcac tgtgtgtcag gaacaatgct cggcctatct tgacctggaa
     8701 ctcgaagacg gtctgcgagc tgaaattgaa taccacctgg aagtgtgccc ggtctgtcaa
     8761 acgtatgtgc atgaattgca ggcagttcaa accgcgttgg ttccactgtc ctcagacgtg
     8821 gccatccctg acgcctggag tcggcaatgg gtggcggccc ttgaacaggc ttcagtgcca
     8881 gaacatcatt cctggtggca gccaggtgcc tggaccgact ggcttcaggc tgctcaaccg
     8941 ctgttggccg gatacggggt tgggattgcc gtgacctgcg tgttgtttag tggcattttg
     9001 ttttcgttca agccaatttt ttcgcttggg acacctgatt acaagatggt gtacatccag
     9061 gccaataccc ggccaacgat ggatccgagc agcattggca tggcgaattt accgacccaa
     9121 ctcgcggcag caaatcagcc gatggactgt gtcattttta tcgctgaagt ttcgccgcag
     9181 gggaaggcgc aactggttga aatggtttct ccgaccagcg accccgaagt tgtcgcccag
     9241 atcagcgatg ctttagggaa aagctcgttt cagccggcaa cctattttgg tcacccggtt
     9301 tcctcccgag tggtgctgat gatcgagcaa atttacgttc gtggatgatt ggtttctctg
     9361 gatagctcag tttcccgttt acatctgacg aagggcgctg tcacatcgtt gacagcgcct
     9421 cattttgttt accccaaaaa gtcctcccac caactggaat cgcgcttgac agacttgaaa
     9481 aaatttgctt attcttacgg ttcgcctttt tatcgaccaa atccagatca tctcagagaa
     9541 agcaattatg tcaacttacg ttcctagcgg caaaaatctg gaagccgagc gcaaatggta
     9601 tgtgattgat gcggcagggc ttaccgtcgg tcggttggcc tctgaagtcg cgctcctgtt
     9661 aatgggaaaa cgcaatccga aatatacgcc atttcttgat cttggcgatc acgtgatcat
     9721 cgtgaacgct gagaaggtgg tgtttaccgg aaataagctg aatgacaagt tctatcggca
     9781 ttacacgttt catcccggcg ggttgcgcga aatttcagcc aaagaccagc ttcagaagca
     9841 tcctgagcgt gttttgaccc tggcggtcaa aggtatgctt cccaaaacca agcttggtcg
     9901 cgccatggct tcaaaactga aagtgtgcat cggtccgacg catccccatc aggcgcaaca
     9961 gccaatccct ttggaattca aataaagttg gtgaagccgc tgctaaccag gcaggcttcg
    10021 cgttgaagga gtggaaatcg tggcaactac acaatattac ggaacaggac gacgtaaaac
    10081 cgcagtcgcc cgtgtttttc tgcgccccgg tgatgggaaa attgtcgtca acaagcgggc
    10141 cttcgagaat tattttccaa acgaagtgca tcgcatggtg attcggcaac ctttgcatgt
    10201 caccgatacg gccagcaaat ttgatctgta cgtcaccgtt cgcggtggcg ggatctccgg
    10261 tcaggccggc gcagtgcgac acggcattac tcatgcctta ctcgccctca acgccgaatt
    10321 ccgccggcgg ttgaagaaag ctgggtttat cacccgcgac ccacgtggga aagagcgcaa
    10381 aaaatacggc caaaaaggtg cccgcaagcg cttccagttt tcaaagcgtt aattcgtttt
    10441 tcaacaacag cttggaatgg tctggtactg ggtgggagtc acatgtgggc ttccacccta
    10501 ttccatcatc gcccagccat gattgcccga caggcgttgg gctctgggtg actgagtaca
    10561 taaatcaacc atctcaactt atctattctc gatagaggaa aggacggtct ctcttggcag
    10621 tcgttgtgac catgaaagag ttactcgaag ctggcgttca ctttggccac caggttcgac
    10681 gatggaaccc gaagatgcgt gaatatatct tcggcgaacg gaacgggatc tacattattg
    10741 acctgcaaaa gacgcagcgg cttttgaagg aatcaatgcg gtttgtgacg caatcggctg
    10801 ccgaagggaa gaacttcctg tttgttggca ccaagcggca ggcacaggaa gccattgctg
    10861 aagaagccct gcggtgcaat atgttttatg tcaaccatcg gtggttgggt ggtttcctga
    10921 ccaacttcca gaccatccag aaatccatca agcgcttaaa agaccttgaa gcgatgcgaa
    10981 ccgatggccg ctatgaagcg ctgaccaaga aggaaatcat taagcgtgaa aaagagcgtg
    11041 aaacgcttga gcgcaacctg gcagggatca aatccatggg ccgcttgccg gatgtgatct
    11101 ttgtgattga taccaaaaag gaagaaattg ccgtggccga agccaaccga ttggggattc
    11161 ccgtcgtggc gattgttgat accaactgtg atccaggtgg gattgacttt gtgatcccag
    11221 gcaatgacga cgcgttgcgt tcagtgcgct tgatcagctc caagattgcc gatgcgattt
    11281 tggaaggtcg tcagatgggg gttgaaaagg ctgaaacacc tgaggttgtc cccgccgctg
    11341 gcggtcgccg tcaggctgac gccgtcgctg tggctgccgc tccggcaccg gaaaccactc
    11401 cccaggcaag tgctgaataa gcaaatgcag aaaaaactgg ctcaatagaa attataaatg
    11461 atgggctatg aatgataaaa cacgtatct
//
LOCUS       DAOQDO010000003        10788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3029_length_10788_cov_3.380602, whole genome shotgun sequence.
ACCESSION   DAOQDO010000003 DAOQDO010000000
VERSION     DAOQDO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_10788_cov_3.380602"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..978
                     /locus_tag="PKZ53_00140"
     CDS             202..978
                     /locus_tag="PKZ53_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ38868.1"
                     /translation="MSNLEKTRRLERIVLDTDENNFATLIVLQNQQVYTSAVLDSLIT
                     SIGRDPECTISLAHDGEASRQHARIRITLTADGNRQYMLSDLGSTNGTFLNGRRLDAN
                     QEVLLNDGDKFIIGQHLFKFAVLDQSNERFMTGNLSQVSLFDLIQVIENNKLTCILII
                     RSQGMVGRLYFNHGQIVDCDLDQKRGEGKVIDSGVESRHGLDAFRQLATIEEGTFEVE
                     KYEYLFPQNIQASSNTNLVLDTLRELDESREKARQERGES"
     gene            1249..3579
                     /locus_tag="PKZ53_00145"
     CDS             1249..3579
                     /locus_tag="PKZ53_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ38869.1"
                     /translation="MPLRTSLQATWLQPASSGTSPEPSTPQSLVPVTAALPATSPPDS
                     EAGAIRLELDVPTLQEPVTAPFVDQPSKPVANEAFRLSQHPTRNLDQPKEIPSSTVGL
                     HSKPSVDPLTSPLTSRKPQLPTAGTQPLPLLPPSSTPGAGTASLTGKPAQPLSPKPTT
                     EGTQPLPSTTALTGPETGTASLVGQSPTEIFWPGSQVVPPQDATSQLTSSSIDPASPA
                     SQETDYLGSRNKKEIPKVFPIGTAPVHPSNETQIDHAQFDPVVDTTQMRAPQTVQQRT
                     PRSVTKPRAASEGGHAARPGKVSGKHKKQLSEIPLADSPASVYEQPPRGAALPTASSN
                     QASTDEADNQVVQLPIPSEDPALRKGSPQYLVKFLESPPTSTLQEVPGSPASFETKPI
                     AETASPELGKGTEPEVSTETPETRFKRTASALHSSGTTPLNSERPDSGTKPHTPGQTS
                     ELTSSSVSAVKPRSTTSPSGGAYLPEKSPVRQKPGRISQNQILALAGAVIGILAIGIL
                     IVWWAVFSQRQPETPPVQPVQQATPVPAPTVPPTGQPAVPALPPDMVLIPAREYLIGR
                     NKGDDPNLDTFETPQHSVKLQAFFLDKTEVTNQQYQEFIKQTGYKAPAQWNGGAFPPG
                     TEQYPVTGVSFEDAEAYARWAGKRLPTEEEWEAAAHGDQIRRYPWGDQFQADRLNSAQ
                     SSLKQPAPVHSYPTGAGPFGNVDLAGNVWEWTASEARPYPGNTEKISNPTQEKLMVIR
                     GGSFENKPPDCVTTYRNWVPATTRYKALGFRCARSL"
     gene            3638..5278
                     /locus_tag="PKZ53_00150"
     CDS             3638..5278
                     /locus_tag="PKZ53_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38870.1"
                     /translation="MTQASKGLISWLTVGVQLGFCVCLFMAGPALAQTVRPKGLGDPS
                     TPPSSTSTKRRPPKKAPSLRTPTGVIVFLGRTPRYYLDQGKQYLQKEDFVSAKIFFDE
                     GLKRTASQRTAPELTEELRRGQQKASLLTEGSRAIEQNQLSQALNAYQQILQLDPTDS
                     FAKQQALKVLQRQIEAAQRQSNWTEMMGYIEQAQQLDPASVADDVFIQALLGQALEAV
                     SRDESAAQALFQRILTIDPTNQIAQQGQKQIETKQLLRTATAALNQRQYTEAENTFTK
                     VLALDSTNEEATHGKNLATAHLIKLRAEAAYQERDFGQAQQLFRDAARTLTDDESILD
                     RLAELQIRLSPKPPTQGTIQLKVPAQTSSRIQLRGNQAFVLSDSGRAEALTTFQMSAP
                     LPYQAFTIRLTSVSNNGRARIQTVPALANQFLTELAVEPKLPGGGEIRIECGWKLITQ
                     GKARWQAQIGPGTYRLLWQGPFVDITTTAGTQPVPNPVVQASLPLQTAEVKVKPVAGA
                     AKVRLVSPGTSLNYFTSTIGLETTQSTLVVIEVEWKIR"
     gene            complement(5566..7302)
                     /locus_tag="PKZ53_00155"
     CDS             complement(5566..7302)
                     /locus_tag="PKZ53_00155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ38871.1"
                     /translation="MTEFHSLFFTDQPGTLAPTDPFVGRTIDKYFIEQRIGRGGMGAV
                     YRAHHTMFDKCVAIKLLSSNLVDDPKTFERFRREATAAARLKHVNVVGVTDFAKTDEG
                     IPYLVMEYVDGYSLRYILDHKPVLSPEQVVALMKQIGAAVHAAHRKGIVHRDLKPDNI
                     MIEISEGEEIIKVLDFGIAKLRDLTTGKSYQSEAESLIGTPQYMSPEQCDGTNLDMRS
                     DIYSLGVMAYEMLTGQVPFTGKPATIMVQHLSKVPLHICSIATDLATAMGDCIMRALA
                     KDPRDRFESALDFALALEKALPQPVDTHLYVLREIIGQPSPEVPVGETSQGVTQTESE
                     YLPFTENLQDVLEAFPVTDLPTDQTKVTLSEAPLMLSPAREAFPPVEGVEPEFRADQE
                     KSHHLSGKRRELYVVTPPTAGLAGRRFMFQPRIVGWVSAVSFLCLLVMAAIGTLIHQY
                     LTYHSLRQKLVSVVGARHPNLKELHQQIEQMAIHQGIELPSQAVKIIVDPPNQQLTVV
                     LKYTSSYCAIPLHYEIEQTVQDYPLTLEELAQVPVGDLQLVNISAIELENYRFEKGLP
                     SHPQQKSEDDQK"
     gene            7671..9113
                     /gene="rseP"
                     /locus_tag="PKZ53_00160"
     CDS             7671..9113
                     /gene="rseP"
                     /locus_tag="PKZ53_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNJ38872.1"
                     /translation="MLLTLLLFLVVLGPMIFIHEFGHFAVAKLLKIKVEVFSLGFGPR
                     LLGFNYGDTDYRVSAIPLGGYVKMKGETLDEEIEGTGDEFLSRPKWHRFLVLIAGPVM
                     NILSAIAIPAAMAMFMFQSPAYLTQPAVVGSVALGSAAEKAGIQPGDRILQIDGKTNP
                     TWGQVSDSTILRPDQPIPVTVQRNTQQLQLTLTPSRIQFGQEKLGDSGLLQNDLISMP
                     IQVKAVSPGTPAEAGGLKPGDKIVKFNGQPVVHFHWFKSSVRNFESKPIDLEVDRNGQ
                     LVTLHITPRQEPRGDVMIGFAPEPSSVPMTPMITQNKSFAAAFRYAVDENIRVLVLTG
                     EALGQIFTGHRAVKETLAGPLGIAKMSSDTYQSLGVEGLLRLMALLSLNLGVFNLLPI
                     PVLDGGHIFIIFLETLFGWVGLQITNGFKEKMMQTGAVLLFLLMGYVIYIDVARLFTE
                     RSATKATTKPTAVDTSPAPATPAPTPAPAR"
     gene            9380..9523
                     /gene="rpmH"
                     /locus_tag="PKZ53_00165"
     CDS             9380..9523
                     /gene="rpmH"
                     /locus_tag="PKZ53_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNJ38873.1"
                     /translation="MKRTFQPNNRRRAKTHGFRARMATKGGRLVLKRRRAKGRKRLTA
                     SHY"
     gene            9676..10137
                     /gene="rnpA"
                     /locus_tag="PKZ53_00170"
     CDS             9676..10137
                     /gene="rnpA"
                     /locus_tag="PKZ53_00170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNJ38874.1"
                     /translation="MGDILNRELPPEHPEAETNTQTNPTTECSLPSTSPSPRPDQQLL
                     KQDRLLRPTEFRRVYDQGLRLHSSLFTIFALHSQFPRVRLGITTSRKIGKAVKRNRCR
                     RLVREVFRHQKTSLLPGWDLVVNAKQPLTKSTYGQVEAEFLRLTKKLSIPR"
     gene            10279..10503
                     /gene="yidD"
                     /locus_tag="PKZ53_00175"
     CDS             10279..10503
                     /gene="yidD"
                     /locus_tag="PKZ53_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNJ38875.1"
                     /translation="MAHFSVRSGAVGLIRAYQRYISPALPPACRFYPTCSQYTAEAIE
                     RYGIWRGGYLGLRRLLRCHPFCPGGYDPVP"
     gene            complement(10565..>10788)
                     /locus_tag="PKZ53_00180"
     CDS             complement(10565..>10788)
                     /locus_tag="PKZ53_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38876.1"
                     /translation="LGVVPGSLRLLVETWLRVIGWKAGKEQSLEVLVWGEMVVVQKSI
                     ASPTKPKKKETTQENNEVFRPYIKNFQSP"
BASE COUNT         2808 a         2906 c         2548 g         2526 t
ORIGIN      
        1 tgcttggaat tgaccccgaa ggagcccagg aagaaccaat aacccaacga gtcataccgg
       61 ccagttcact gatgccgacg atccgggcat cagcgctccc cccacctgat tcggagccga
      121 caaactcttg agagattttc acgatctgcg ccctgaagta ttttttgatt tgccgaaccc
      181 agtccacgca cgaggcgtcc tatgagcaac ctggaaaaaa cccgccgact tgaacgcatt
      241 gttttagaca cggacgagaa taatttcgca accttaattg tgctccaaaa ccagcaggtc
      301 tatacctcag ccgtactcga ttcattgatc acttcaattg gacgagatcc tgaatgtacc
      361 atctcgctgg ctcatgatgg ggaagcctca cgccagcacg cccgaatccg aattacatta
      421 acggcagatg ggaaccgaca gtacatgctg tcagacctgg ggagcaccaa tggtacgttc
      481 ttgaatggac gccgattgga cgccaaccag gaagtgctcc tcaacgatgg ggataaattt
      541 attattggtc agcatctgtt caagtttgcg gtgctcgatc agtccaacga acggtttatg
      601 actggcaatt taagccaggt cagcctcttt gacctcattc aagtcattga aaataataaa
      661 ctcacctgta tccttatcat tcgctcacag ggcatggtgg ggcgactcta ctttaaccac
      721 ggccaaatcg ttgattgtga tcttgatcaa aagcgtggtg aaggaaaagt gattgacagc
      781 ggcgtagaga gcaggcacgg cctggatgcc tttcgtcaat tggccacaat tgaagaagga
      841 acatttgaag ttgaaaaata tgaatacttg ttcccgcaaa atattcaggc ctccagcaac
      901 accaatctgg tgctcgatac gttgcgagaa cttgatgaat ccagagaaaa agcacgccag
      961 gagcgtggcg aaagttaaga ggccaggcac ggttcgtagc ttctggactt ttgaaaacgt
     1021 tattggtgaa gcataaaaca ccaggttgct ccaatcagca accagttgat tgcgaccgga
     1081 tgtgagccag ggtcatcctt attccccacc ccagggggat ccattatgtt ttgtccttat
     1141 tgcagtactc aaaatcgcga caaagcacgt ttttgtagtg ggtgtgggag aactcttaca
     1201 gagtcacctt ccgggctcag ttcattgagc ggtggtctgt catcgcccat gccgctgcga
     1261 acttccctcc aggcaacctg gctgcaacca gcatcgagtg gaacctcacc ggaaccatcc
     1321 acccctcagt cgctggttcc ggtgacagcg gctttgccag cgaccagtcc accagattca
     1381 gaagcgggtg ctattcgatt ggaactggat gttccgacct tgcaagagcc cgtcacggct
     1441 cccttcgtcg accagccatc caaacctgtg gccaacgaag catttcgact ctcacagcat
     1501 cccacccgaa atctggatca accgaaagaa ataccatcgt caaccgttgg attacactcc
     1561 aaaccatcag ttgatccatt aaccagcccg ctgacctctc gcaaaccgca actcccgacc
     1621 gccgggacac aaccgttgcc actgctcccc ccctcttcaa cgccaggggc tggaacggct
     1681 tccctgacag gaaaacccgc gcaaccactc tcacccaaac cgaccactga aggtacccag
     1741 ccattaccgt caacaacagc tctaactgga ccagaaacag gcacggcgtc gctggtgggg
     1801 cagtcaccaa ctgaaatctt ctggccagga tcccaggtgg ttccgcctca agatgcaacc
     1861 agtcaactca ccagcagttc gattgacccg gcctcacctg ccagccagga aaccgactat
     1921 ctgggaagtc gaaacaagaa agaaattccc aaagtttttc cgattggcac agcgccggtt
     1981 catccttcga atgaaaccca aattgatcat gctcagtttg atccagttgt ggatacgacc
     2041 caaatgcggg cgccccaaac agttcagcag cgcacaccac gaagcgtgac caaaccccga
     2101 gctgccagtg agggtggcca tgcggctcga ccaggaaaag tcagtgggaa acataaaaaa
     2161 cagctatcgg aaattccgct ggctgattcc cctgcgtctg tatatgagca acctcccaga
     2221 ggagccgcct tgcctacggc ctcatcaaat caggcctcaa ctgatgaagc cgataaccag
     2281 gtcgttcagc tcccgatccc atctgaggat ccagccttgc gaaaagggtc accacagtac
     2341 ctggttaagt ttttggaatc gcctccgacg tcaacgcttc aggaagtccc tgggtcacca
     2401 gcttcctttg agaccaaacc aattgctgag acagcctctc ctgaactcgg gaaaggaact
     2461 gagcctgaag tatcaactga aacccctgag accagattta aacgtactgc atcagcactc
     2521 cacagtagcg gaacaacgcc gctcaacagt gagcgaccag actctggcac aaaaccacac
     2581 acaccaggtc agacctctga actcacatcc tcctctgttt ccgcggtgaa accgcgatca
     2641 acgacgtctc cttctggtgg cgcctacctt ccagaaaagt cacctgtccg ccaaaaacct
     2701 ggtcgaatca gtcaaaatca aattctggct ttggcggggg cggtgatcgg gattctggcc
     2761 attggcattt tgattgtctg gtgggccgtt ttttcacagc gccaaccaga aaccccaccc
     2821 gtacaaccgg tccagcaggc caccccagtc cccgctccga ctgtgcctcc aactggtcaa
     2881 ccagccgtac ccgcccttcc gccggatatg gttttgatcc cagccaggga atatctcatt
     2941 ggacgcaaca aaggggacga tccaaatctt gacacgtttg aaacgccaca acacagtgtc
     3001 aaattacagg cattctttct ggataaaaca gaagtgacca atcagcaata tcaggaattt
     3061 atcaaacaga ccgggtacaa agctccggca caatggaatg gaggagcgtt tccacctggc
     3121 accgagcaat atccagtgac cggggtatca tttgaagatg ctgaagccta tgcccggtgg
     3181 gcgggaaaac gacttccgac cgaagaagaa tgggaggctg ccgcccacgg cgatcagatt
     3241 cgacgctatc cctggggaga tcagttccag gctgaccgat taaactcagc ccaatccagc
     3301 ttgaaacaac cggcgccagt tcattcttat ccgaccggcg ctggaccatt tggcaatgtt
     3361 gatctggctg gaaatgtgtg ggagtggaca gccagtgaag cccggcccta cccaggcaat
     3421 actgaaaaaa tctctaatcc gactcaagaa aaactgatgg tgattcgcgg tggatcattt
     3481 gaaaataaac cgcctgattg tgtcacgacc tatcggaatt gggttcctgc cactacgcgc
     3541 tataaagcac ttggatttcg ctgtgccagg tcgctgtaag gcgagtatcc tgccctcaaa
     3601 tctcactcat tctcaccaaa ttcaggtgat gtgcgccatg actcaagctt ctaaaggatt
     3661 gatttcctgg ttgacagttg gtgttcagct tggcttctgt gtgtgccttt tcatggcagg
     3721 tccagctctg gctcaaaccg tccggcccaa aggattggga gatccatcaa ctcccccctc
     3781 ttccacctca accaaacgtc gccctcccaa aaaggcaccc agtcttcgaa caccaaccgg
     3841 tgtcatcgtt ttcttaggtc gcacgcctcg ctactatctt gaccagggaa agcaatatct
     3901 tcagaaagaa gactttgtat ccgcaaagat tttttttgat gaaggactga aacgaacagc
     3961 cagccaacga acagcacccg aactcacaga agagcttcgt cgtgggcaac agaaagcatc
     4021 ccttttgact gaaggaagca gagcaatcga acaaaaccag ttgagccagg cgctcaatgc
     4081 ctatcagcaa attctgcagc ttgacccaac tgacagcttt gccaaacaac aggcgctcaa
     4141 ggtgttgcaa cgccagattg aagccgccca aagacagtcc aactggactg agatgatggg
     4201 ttatattgag caggctcagc aacttgaccc ggcatcagta gcagatgatg tcttcattca
     4261 ggcactgctg gggcaggcac tcgaagctgt tagccgggat gaaagtgcgg ctcaggctct
     4321 ttttcagcgc attctgacca ttgacccaac caatcagatt gcccaacagg ggcaaaaaca
     4381 gatcgaaacc aaacaactcc tccgcacagc aaccgccgcg ctcaatcaac gtcaatatac
     4441 cgaagccgaa aacactttca cgaaagtact ggccctggat tcaacgaacg aagaagcaac
     4501 ccacggaaaa aacctggcaa cggcgcatct gatcaagctt cgggctgagg cagcctatca
     4561 ggaacgcgac tttggccagg cgcaacagct ttttcgagat gcggcaagga cattgaccga
     4621 cgatgaatca attctggacc gacttgcaga acttcagatt cgattatctc ccaaaccacc
     4681 gactcaggga accatccagc tcaaagttcc agcccaaacc agtagtcgaa ttcaactccg
     4741 aggaaatcag gcatttgtgc tctctgactc aggcagagcc gaggcgctta cgacattcca
     4801 gatgagtgct ccactgccat atcaggcatt taccattcgg ttaaccagtg tttccaacaa
     4861 tggacgggcc cgaatccaga cggttcccgc cctggctaac cagtttctga ccgaactggc
     4921 agttgaacct aaactccctg gtggtggtga aattcgaatt gaatgtggct ggaaacttat
     4981 cactcaaggc aaagcccgat ggcaggctca gattggtcct ggtacctatc ggttactctg
     5041 gcaaggccct tttgttgaca ttactaccac cgctggaacc caacccgtgc caaacccagt
     5101 ggtgcaggcg tcgctcccac tgcaaaccgc agaggtaaaa gtcaaaccag ttgctggggc
     5161 ggccaaggta cggctggttt cgccagggac gtcgttaaat tattttacgt caacgattgg
     5221 gcttgaaacg acacagtcca ccctggttgt catcgaagtt gagtggaaaa ttcgctaatt
     5281 ttggagggaa tgaactgaag gggaaagaag ggctttgtgt tttgggagga atgcgctata
     5341 aaaaataatg gccgcaccgt aaacttatcg acagaccccg cacgacgctg tcgataagat
     5401 taggtgcgac cctagaataa catctcggat ttacctaata caactggcgt gccactcttt
     5461 cagattaaca ataacccacc tgcaatcaac aatataacct gccatgccat gagcctccca
     5521 ctcaaaatct gacaaaaatc gtcacattcc atcagcctct ttttgtcact tctggtcatc
     5581 ttcagatttt tgttgcggat gagacgggag gcctttctca aatcgatagt tctcaagctc
     5641 aatcgctgaa atattcacca actgaagatc acccaccggc acctgagcca gttcttccag
     5701 ggttaaggga tagtcctgga cggtttgttc aatttcatag tgaagcggaa tggcacaata
     5761 tgaactggtg tattttaaaa ccacggtgag ttgttgattg ggaggatcaa caatgatttt
     5821 cacggcctgg gatggcaact caatcccctg atgaatagcc atttgttcaa tttgctgatg
     5881 aagctctttt aagttgggat gacgtgcgcc cacaaccgag accagttttt gacggagtga
     5941 gtgataggtc aaatattggt gaatcagtgt tccaatcgcc gccatcacaa gcaggcaaag
     6001 gaaactcacc gcactcaccc accccacaat ccgaggttga aacataaaac ggcgaccagc
     6061 cagccctgcg gtgggaggag taaccacata caactctctg cgtttcccac tcaggtgatg
     6121 gctcttttct tgatcggcac ggaattcagg ttcaacacct tctaccgggg gaaaagcctc
     6181 gcgtgccgga gaaagcatca aaggtgcttc tgacaatgtg accttcgtct gatctgtggg
     6241 caggtcagtc accggaaatg cttccaatac atcctggagg ttctcagtaa acggcaggta
     6301 ctcactttca gtctgggtca caccttgcga tgtttcgcca accggaactt cgggtgaggg
     6361 ctggccaatg atttcccgca atacatacag atgggtgtca accggctgag gaagtgcttt
     6421 ttccagagcc agggcaaaat caagggctga ttcaaaccga tctctgggat cttttgccag
     6481 tgcccgcata atgcaatcgc ccatcgcggt ggcaagatca gtggcaattg aacaaatatg
     6541 cagtggaact ttggaaagat gctgaaccat gatggtggcc ggttttccag tgaacggcac
     6601 ctgcccggtc aacatctcat aggccataac gcctaaactg tagatatcag agcgcatgtc
     6661 aaggttggtg ccatcacatt gctctggaga catatactgc ggagttccaa tcagactttc
     6721 agcttcagat tgatagcttt ttccagtggt gagatccctg agtttggcaa ttccaaaatc
     6781 aagaacttta ataatctctt caccttcaga gatctcaatc ataatgttgt caggtttcag
     6841 atctcgatgg acaatcccct ttcggtgcgc ggcatgcacg gcggccccaa tttgtttcat
     6901 caaagcaacc acctgctcgg gtgagagcac tggtttatga tcaaggatat agcgcagtga
     6961 gtacccgtca acatactcca tgaccagata gggaatacct tcatctgtct tcgcaaagtc
     7021 tgtcacacca accacgttga cgtgtttgag tctggcggcg gcggttgcct cacgacgaaa
     7081 tcgctcaaat gttttcggat catcaaccag atttgaagac aggagcttaa tggccacaca
     7141 tttgtcaaac atggtgtggt gggcacgata aaccgccccc ataccacccc ggccaatgcg
     7201 ctgttcaata aaatatttat ctatcgttcg gccaacaaat ggatcggtgg gtgccaaagt
     7261 tcccggctgg tcagtaaaaa acaatgaatg aaattcggtc atggtgattg aaacagctca
     7321 gcctgaaagc ctggaggaaa atgctttcag tcattgtcaa tcaatcgctt gagacatcca
     7381 atcttgggcc tcgccatcaa agcgattaaa ggactcttgg ttaccctggc tgaaaaacga
     7441 tgcgcggttg cgagtgcaac gcggaaacag gttggaggta ccagcagaaa gtaccatatc
     7501 atttaacatg gaacctcaaa cagagacaac ctctttcgca gtatttgcca aaggccagga
     7561 acatcttgtt gcagcaaaag cgtttcagga ttttgtgctg ccgaaaccac cacgctcgtg
     7621 gtacagttta tgattccaag cctatagttt ttatgaagga gtagagaggg atgctgctaa
     7681 ccctgttgtt gtttttagtt gtgctcggtc cgatgatctt tatccatgaa tttggtcact
     7741 ttgcggttgc caagttgttg aaaatcaaag ttgaagtgtt ttcactcgga ttcgggcccc
     7801 gcctgcttgg gtttaattat ggagatactg attatcgggt ttcagcgatc ccgcttggcg
     7861 gatatgtcaa gatgaaaggt gaaacgctgg atgaagaaat cgagggaaca ggtgatgagt
     7921 ttctctcacg tcccaagtgg catcggtttc tggttctgat tgccgggcca gtcatgaata
     7981 ttctcagcgc gattgcgatt ccggcggcaa tggccatgtt tatgtttcaa tctccagcct
     8041 atctcaccca gcctgccgtg gtgggaagtg tggcactggg gtcagccgct gagaaagctg
     8101 ggattcaacc cggggataga atccttcaga ttgacggcaa aacaaaccct acctggggac
     8161 aagtctctga ttcaaccatt ctgcgccccg atcaacccat cccagtcacc gtccagcgca
     8221 atacccagca actccaattg acgttgacac cttctcgcat tcaatttggc caggaaaagc
     8281 ttggtgattc gggactgctc caaaatgacc tcatttcaat gccaatccag gtcaaagcgg
     8341 taagccctgg aactccagcc gaagctggtg gtctcaaacc tggggataaa attgtcaaat
     8401 tcaacgggca acccgttgtt cattttcatt ggtttaagag ttcggtgcgc aattttgaaa
     8461 gtaaaccgat tgacctcgaa gttgatcgca atggtcaact ggtgaccctg catatcacac
     8521 ctcgtcagga accccgaggg gatgtcatga ttggatttgc tccagagcca agttcagttc
     8581 cgatgacacc aatgattacg cagaacaaat ccttcgcggc agctttccga tacgccgttg
     8641 atgaaaacat tcgggttctg gtactcacag gtgaggcgct tgggcagatt tttaccgggc
     8701 accgggccgt gaaagaaact ctggctggac cgctggggat cgccaaaatg tcttctgata
     8761 cctatcaatc ccttggggtt gaaggcttac tccgattgat ggcgttgttg agcctcaatc
     8821 tcggagtctt caatttgctt ccaattccag tcctggacgg cggtcatatt ttcatcattt
     8881 tcctggaaac actctttggc tgggttggat tgcaaatcac aaacggattt aaggaaaaaa
     8941 tgatgcagac gggcgctgtt ctgctctttt tattgatggg gtatgttatt tacattgatg
     9001 tggcccgatt atttaccgaa cgatcagcca ccaaagcaac caccaaaccg acggcagtag
     9061 acacctcccc agcaccagcc acaccagccc caacccccgc tccagccagg taaatcaacc
     9121 agctcaactc attcctggta aatcagttcc ctgatttacc aggatcaaca cccttcccaa
     9181 gtcgaaattc ttcgcttgac gaaatctccc cccatctgtc ctctcacgtt tttgtctaaa
     9241 aattcaaaaa ttcgactgac gcacctctta tacctggagc agattggtca ctggctggcg
     9301 gctggtgagt tcttatccag gaggtttgga tttcgaatat tgaacattca tcatcccagg
     9361 aagcgaagga tttggatcta tgaaaagaac attccaacca aataaccggc ggcgtgccaa
     9421 aacccatggg ttccgggccc gcatggccac caaaggtggt cgtcttgttt taaaacggcg
     9481 acgcgcaaaa gggcgtaaac gcctgacggc ttctcattat taaaaggtgc ttcagttgaa
     9541 gtacaggttc tttgtcgccg acccaccagt ttgctttcac ctgacggcca gtatgtagat
     9601 atactctacc gatcaaggta gaagcattgg cgtttttatt cggcgcgatt tgtactcatc
     9661 ccgaaccaga ttgatatggg agatatcctg aaccgagagt tgccaccaga gcaccctgaa
     9721 gccgaaacaa acactcagac caacccaacc accgagtgtt cacttccatc gacttcccct
     9781 tcgccacgac cggatcaaca gttgttaaag caagaccgct tgcttcgccc aactgaattc
     9841 cggcgtgtct atgaccaggg tctgcggttg cactcttcac ttttcacaat ttttgcattg
     9901 cattcccagt tcccacgcgt ccgccttggg attaccacca gtcggaaaat cgggaaggca
     9961 gtcaaacgca accgttgccg acggctggtc agagaggtgt ttcgacatca gaaaacatct
    10021 ttgttgccag ggtgggacct ggtggtaaat gccaagcaac cattaaccaa gagtacctat
    10081 ggacaggtgg aggctgagtt tttacggctg acgaaaaagc tctccatacc caggtgatcg
    10141 taaataattc acccactaaa atcggaccgg gggtcggagc gcctggcgtt tatcacctga
    10201 ccctgggcaa ggtacccaga ctcatcattc aaccgttctt cgcgataacc tgaaaaacat
    10261 tgatgggttg gcctggatat ggcgcatttt tcggtacgat ctggagcagt tgggttaatt
    10321 cgagcgtatc aacgctacat ctccccagcc ttgcctcctg cctgccgatt ttatcccacc
    10381 tgttctcagt acacggctga ggcaatcgaa cgctatggga tttggcgggg cgggtatttg
    10441 gggctgcgac ggttactccg gtgtcatcct ttttgtcctg gtggatatga tccggttcca
    10501 tgaatagatc tgtgctttga cacagaggtg tgctgtttta tttcgatcct gaatcagcag
    10561 cacgtcaggg gctttgaaag tttttgatgt atgggcgaaa aacctcgtta ttttcttgcg
    10621 ttgtttcttt cttttttggt tttgttgggc tggcaatact tttttgcacc accaccatct
    10681 ccccccaaac cagcacttcc agcgactgct ccttcccagc cttccaccca atcaccctca
    10741 gccacgtctc aaccagcagc cgtagcgagc cagggaccac tcccaact
//
LOCUS       DAOQDO010000004        10541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3143_length_10541_cov_3.370875, whole genome shotgun sequence.
ACCESSION   DAOQDO010000004 DAOQDO010000000
VERSION     DAOQDO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3143_length_10541_cov_3.370875"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKZ53_00185"
     CDS             complement(<1..275)
                     /locus_tag="PKZ53_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ38877.1"
                     /translation="MQSPNLPPPSVPEIEQWLKTTELFQGFPADLIQDMSAELQRIRL
                     AKDAVLFRQGDIADAIYLVLTGTLRVTVKQAERQEQIVGMVSTGQPVG"
     gene            complement(437..1327)
                     /locus_tag="PKZ53_00190"
     CDS             complement(437..1327)
                     /locus_tag="PKZ53_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNJ38878.1"
                     /translation="MELRHLRYCIAVADELNFNRAAEKLHVSQSALSKQIRELEEEVG
                     IEIFERTKRKVEVTEAGRLFLAEARRTLAQADQTLQIATRAGRGEIGKLVIGLEGSST
                     YDLIPLILHTYRSRFPQVELVVTEMPTLQQVYALEHKHIDVGFLIQPGDLADNIECER
                     LLQEPLIVALHQSHPLKNQPYVSLQALAKDAFVFVPQSLGCGFFSLVSQIFRSAGYTP
                     GIIQEASQLQTLLSFVAAGMGITLIPASARVFQMPGIVYVDLEPPAPTVDVMVAWRGD
                     DSSPVVRTFLKIVRNNAQTQ"
     gene            1577..2137
                     /locus_tag="PKZ53_00195"
     CDS             1577..2137
                     /locus_tag="PKZ53_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNJ38879.1"
                     /translation="MGRVAIVYHSGYGHTAAQAEAVARGAASVEGTTVEVFTSDDAIT
                     SLEKLNDFDAIIFGSPTYMGSVSSKMKAFMEASSKMWMELKWKDKIAAGFSNSGSLSG
                     DKLNTLLQFAVFAAQHAMIWVGIDVLPSGAPGDPESINRVGSFLGAMAQSTHGTAVPP
                     DGDLKTAELLGRRVALTAQQWVRGRQ"
     gene            2284..3009
                     /locus_tag="PKZ53_00200"
     CDS             2284..3009
                     /locus_tag="PKZ53_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNJ38880.1"
                     /translation="MITVRPGTERGQTQTYWLDSRHSFSFNMYFDPKQMGFQHLRVIN
                     EDWIRPGAGFGMHSHADMEILTYVIKGTLAHKDSTGGVGVIQAGEVQRMSAGTGISHS
                     EFNHSDTEEVHLFQIWLLPERKGLTPGYEQRQFATADRQDTLCLVAARDGRNQALTIY
                     QDVDFYVASLTAPGKTVSHRLCPTRHGWLQVGLGSVKVNGIELKTGDAAAISDVEHVE
                     IAALEPSEILFFDLANSPKPNRH"
     gene            3228..3899
                     /locus_tag="PKZ53_00205"
     CDS             3228..3899
                     /locus_tag="PKZ53_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNJ38881.1"
                     /translation="MTIQEVLQQLEALGTEQARKVYRQHGISTPMFGVSYADLGKLQK
                     KLKINHDLAVELWNSGNHDARILATMIADPKQLTLEQIEACAADLSDYALTDALAGLV
                     AKTSGARQVLDQWKDSDQEWPGQLAWGILGRLAASDSTLTDEFFTPYLHTIEQEIHTR
                     PNRVRHAMNGALIGIGMRNAQLEAEALRIAAAIGKVKVDHGQTSCKTPDAASYIKKAR
                     ARSAR"
     gene            complement(4284..5207)
                     /gene="arsN2"
                     /locus_tag="PKZ53_00210"
     CDS             complement(4284..5207)
                     /gene="arsN2"
                     /locus_tag="PKZ53_00210"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HNJ38882.1"
                     /translation="MEFAKDIERIGAILRETARLHLQLQRLAVASCDQTTSTQCFIIS
                     ELGRTGTLTLADLARRVGLDKSWTSRAVESLVQEGLIAKVQSDEDRRLVHLSLTEAGH
                     QRFENLNAVLDTQAERVLNRIDAIQQPQIFEVLRLIRDALRTELSGSVVGGVSDTHLD
                     SGLSFQPVGSDQLEFIRSLLIASRLPVDGIESPNLEFTSVSRNGKLVGCAGLERYGKD
                     ALLRSVAVAESERNSGIGKQLVLRLLDQAAQQRLKKIVLLTTTAETFFRRFGFQKIPR
                     SQAPRSVLGSVEFQTVCPSSATVMVYRVPRP"
     gene            complement(5448..5840)
                     /locus_tag="PKZ53_00215"
     CDS             complement(5448..5840)
                     /locus_tag="PKZ53_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38883.1"
                     /translation="MEIKVVLCNCKGMCTSFKDADMNTLPFQIESGLDVTYSLVNPQL
                     CGQGGNAVLMDIFQSAGPDTYILSGACAPEVQSKLFKRIIRHTGFNEAHFYPVDIRGT
                     TNEGILERLRTAVAEIIARENTPVEIAK"
     gene            complement(6233..6937)
                     /locus_tag="PKZ53_00220"
     CDS             complement(6233..6937)
                     /locus_tag="PKZ53_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ38884.1"
                     /translation="MQEPKVAIITAASRGMGAACARELANRGYKLALMSRSTEAEDLA
                     SELGGFGITGSVTEETDIRRLVTATLEKYGRIDAVVNNTGHPAKGELLNIQDEDWHKG
                     LDLLLLNVVRMSRLVTPVMELQGGGGLVNISSFTMRMPSLPMPVSSVLRAALSSFTKL
                     YADRYARANIRMNCILPGYVENYEISDETREAIPMNRPAQLQEIARTVAFLLSEEAGY
                     ITGQSVTVDGGLTRSY"
     gene            complement(6924..7427)
                     /locus_tag="PKZ53_00225"
     CDS             complement(6924..7427)
                     /locus_tag="PKZ53_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNJ38885.1"
                     /translation="MDPQPNQVPAAQILNQAVEALLVETLEMFFTLRAAGKRIGAVTQ
                     SGGGYWGMLRLLKTTGPRTVPQLARMRSVTRQRMQTLANELAQDGVIEFVANPDHQRS
                     KLVQLTAHGEAVFQELSTTISTEISRLSHDLEVTEVETAIQVLQHLRQCLQPSLKSQR
                     NTHDAGT"
     gene            7607..8224
                     /locus_tag="PKZ53_00230"
     CDS             7607..8224
                     /locus_tag="PKZ53_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ38886.1"
                     /translation="MGYIASQFGNPRGLVGKWIGWLMAIKNNDRIDWTCSQLGVRPGD
                     RLIEIGYGPGVAIEKLIQSTPQIQIVGIDHSETMFAQAYRRNLKVIQTGQVTLHVGTV
                     APLPLADCSFHKALTINSSFFWPDPVEGFRELLRVLKPEGKIAVTVQPFFAKTDDQFQ
                     KAGTTLVSQLREAGFQLISLDRKHMRPVDCWCAIGLKKRIEPSIF"
     gene            8292..9215
                     /locus_tag="PKZ53_00235"
     CDS             8292..9215
                     /locus_tag="PKZ53_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ38887.1"
                     /translation="MKIFVTGGAGFIGSHVADAFLNLGHKVVIFDNLSTGRRENLNPQ
                     AKFIEGDLGDDSLPDILKAESPDVISHHAAQIDVRKSVTDPRFDAQVNILGSIHLLEA
                     ARQAGVKHVMFASTGGAIYGEQDYFPADENHPLRPCSPYGIAKLSVEKYLDYYRLVYG
                     LKTTILRYANIYGPRQNPHGEAGVVAIFCERMLSGKPLVINGEGLQTRDYVFVGDVVK
                     ANLFTINDDHSDIYNVGTGVETTVVDIFHHLREASGLDIAENHGPAKAGEQLRSVLTS
                     RKLFEHFGWKPQMPIAEGLKLTLESFRQPVQ"
     gene            9250..10308
                     /gene="rfbB"
                     /locus_tag="PKZ53_00240"
     CDS             9250..10308
                     /gene="rfbB"
                     /locus_tag="PKZ53_00240"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNJ38888.1"
                     /translation="MQNLLVTGGCGFIGSNFIRYLLEESDFTGRVINLDALTYAGNPE
                     NLADLAARSPQRYTFVQADICDAAKVAEIFDSHQIDTVCHFAAESHVDRSIVNPGAFI
                     QTNVVGTYTLLEAARQRKDRISLFHHVSTDEVYGSLGEEGFFTEDTPYRPTSPYSASK
                     AGSDHLVRAYFKTYGLPVTISNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGDGRN
                     VRDWLYVRDHCTAIWTVMNRGQRGQTYNIGGHNEIRNLTVVETICTLLDEMVEPLPTG
                     PRSNLITFVKDRPGHDWRYAIDSTKIQQELSWTPEESFETGIRKTITWYLENRTWVDH
                     VRSGEYQTWMAAQYGVTG"
BASE COUNT         2694 a         2636 c         2637 g         2574 t
ORIGIN      
        1 ccaaccggtt gcccggttga aaccatgcct acaatttgct cttgcctttc ggcctgcttc
       61 accgtgacgc gaagcgttcc ggtcagaacc agataaatcg catcggcgat atcaccttga
      121 cggaacagaa cggcatcttt tgccaatcga atgcgttgca gttccgcaga catatcctgg
      181 atcaggtcag ccggaaaacc ttgaaacagt tcagtggttt tcaaccattg ttcgatctct
      241 ggaacggaag gaggcggaag attgggagat tgcatgggcc tggatgtatg agtgagaaat
      301 gaaaaattga gaatgaagaa tgaagaatga agaatgagga atgaagaatg aagagtgatt
      361 attcaatcat cggatcattg tgtcatttgg gccattctgc catttctcaa tttgtttcag
      421 caagttatgt gttccgtcat tgcgtttgag cgttgttgcg cacgattttg agaaacgtgc
      481 ggaccacggg tgatgaatca tcaccccgcc aggccaccat tacatccacg gtcggcgctg
      541 gcggctccag gtcaacatac acaatcccag gcatttgaaa cacccgggcc gacgccggaa
      601 tcagggtgat gcccattccg gcggcgacaa aactcaacaa ggtttgaagt tgactggctt
      661 cctggatgat tcccggtgtg tagccggctg atcgaaaaat ctgactgacc agcgagaaaa
      721 acccgcagcc caggctttga gggacaaaca caaaggcatc ttttgccagc gcttgaagtg
      781 agacatacgg ctggtttttc aatggatgcg actgatgcag ggccacaatc aacggttcct
      841 gcaggagccg ttcacattcg atattgtcag ccaggtcgcc cggttgaatc aaaaatccaa
      901 catcaatgtg tttgtgttcg agggcataca cctgctgcag cgtcggcatt tcagtcacca
      961 ccagttcaac ctgtggaaag cgggaccgat aggtatgcaa aatcaatgga atcaggtcat
     1021 aggtggacga gccttcgagg ccaatcacca gtttcccaat ttcaccacgc ccggcgcggg
     1081 tggcaatctg gagtgtctga tccgcctggg ccagcgttcg gcgggcttcg gccagaaaga
     1141 gccgaccagc ttcggtgact tcgactttgc gtttggtgcg ttcgaaaatc tcaattccga
     1201 cttcttcttc gagttcgcga atctgtttac tgagtgccga ttgtgaaacg tgtagctttt
     1261 cagccgcccg gttgaaattg agttcatcgg ccacagcgat gcaatagcga aggtgtcgga
     1321 gttccatagg gttggtttac aggcaaagga aaaggacaaa aaggacaaaa aggaccaaag
     1381 agacgaaaag gaccaaagag gttccttgaa tgtttatcga aaacccggaa cccggaaccc
     1441 ggaacccgga acccggaacc cgagactaat ctctttgagc gattaagtga tgagaacaat
     1501 gtattggact cctagcgtgg aaactgggta ttgtcaacgt tgttaggaat ccggatttca
     1561 tcacaaagga gtgtatatgg gacgtgttgc aattgtgtat cacagtggat atggacatac
     1621 ggcggctcaa gccgaggctg tcgcccgagg cgcggcatct gtcgaaggta ccacggttga
     1681 agtttttact tcagatgatg ccatcaccag cttggaaaag ttaaatgatt ttgacgccat
     1741 catctttggt agcccaactt atatgggcag tgtttccagc aagatgaaag cctttatgga
     1801 agccagttca aagatgtgga tggagctgaa atggaaagac aaaatcgccg ccggtttctc
     1861 gaattccggg agcttgagcg gcgataaact caacaccttg ctccagtttg cggtatttgc
     1921 agctcaacac gccatgatct gggtgggaat agacgtgttg ccatctgggg cacccggcga
     1981 cccggaaagt atcaatcgcg tcgggagttt tctcggtgcc atggcgcaat ccacccacgg
     2041 aacagccgtc ccgcctgacg gcgatttaaa aacagcggaa ctgcttggcc gccgggtggc
     2101 actcactgcc cagcaatggg tacgtggaag acagtgacaa aagaacgggc ttggggctga
     2161 agacatcggg ctgagggctg agggtgacac ctaactgaat tcaaatgatt tgtataccct
     2221 gagccctcag ccccaagcct tggtttcttc agccccaggc cccaggatta aaggaggttt
     2281 caaatgatta ccgtccgacc aggaactgaa cgcggacaaa ctcaaaccta ttggctcgat
     2341 agccgacatt cgttctcatt taatatgtat tttgatccga agcagatggg atttcagcac
     2401 ctgcgagtga tcaatgaaga ctggatccgg cccggtgctg gttttggaat gcactcgcat
     2461 gccgacatgg aaattttgac ctatgtgatc aaaggcaccc tggcccataa agacagcacg
     2521 ggtggcgtag gtgtcattca ggcgggtgaa gtccagcgga tgtccgccgg aaccggcatc
     2581 agccacagtg aatttaatca ctctgatact gaagaagtgc atttgtttca aatctggttg
     2641 ctgcccgaac gcaaaggatt gacgcccgga tatgagcagc gtcaatttgc caccgcagac
     2701 cgccaggata cgctctgtct ggtggcagcc cgtgatggac gcaaccaggc actgaccatt
     2761 tatcaagatg tagattttta tgttgccagc ctgacggcac ccggaaaaac cgtcagccac
     2821 cgtctctgcc caacccggca cggatggctt caggttggac ttggaagtgt gaaagtcaat
     2881 ggaattgaat taaaaaccgg tgatgcggcg gcgatcagcg atgttgaaca tgttgaaatt
     2941 gcagctcttg aaccctctga aattcttttc tttgatctgg cgaatagtcc aaagccaaat
     3001 cgtcattgag agttgagctc tcatcttact cagaaacagc caccctggtt ttttcgacca
     3061 gggtggctgt tttcatttgc gatcagcctg tggatgagcg tcagttacgg gtgatgtctg
     3121 agcagccgaa aaattttacc ttcaaccagt gattttgctc tggacatagc taagtaattt
     3181 tcagatgata ttcactgcaa cctattttca aactcaggag catgcccatg acgattcagg
     3241 aagttttgca gcaactggaa gcacttggaa cggaacaagc ccggaaagtt taccgccagc
     3301 atggtatcag cacgccaatg ttcggtgtca gttatgccga tctcggaaaa cttcaaaaga
     3361 aactcaaaat caatcatgac ctggctgttg aactttggaa ctccggcaac catgacgccc
     3421 ggatcctggc cacgatgatt gccgacccga aacaactgac gctggaacaa attgaagcct
     3481 gcgccgctga tttatccgat tatgccctca cggatgcttt ggccggactg gtcgcgaaaa
     3541 catcaggtgc cagacaggtc ctggatcagt ggaaggacag tgaccaggaa tggcctggac
     3601 aattggcctg gggaattttg ggaaggcttg cagcttcaga ttcaacactc acagatgagt
     3661 ttttcacacc ctatttgcac acgattgagc aggaaattca cacccgacca aaccgcgtcc
     3721 ggcacgcaat gaacggcgcc ttaatcggca tcgggatgcg caatgctcaa cttgaagccg
     3781 aagcgcttcg aattgcggcg gccattggca aagtcaaagt tgaccatggc caaaccagtt
     3841 gcaaaacccc ggatgccgcc agctatatca aaaaagccag ggcgcgaagt gccagatgag
     3901 aggtgtcagt accacctgcg tgagcgggcg gggttggaag caccagaccc acccactgat
     3961 gcgggtggta ctgatggagt ggtgcgtctg atttgcctcc aacacaaaat ctggtaagta
     4021 ctttaccgaa aatttccaga cattttaacc acggaaaaca cggaaaacac ggaaaaaaat
     4081 caaccactta ccaaatccaa tatctcagga aacttatgac aaggtactta caagcagaaa
     4141 tgttcgactg cgaacaattc ctataatcaa tttgttttca aagagataaa aacaaaaata
     4201 tttattcgtt acctgaataa aaaaggcaca aaccgttcaa aagtgagtgc cttccctcaa
     4261 aatctgactt ctttcacaac ctttcagggc ctgggcacac gatacaccat gacagtggct
     4321 gatgacggac agacagtttg gaactcaacg gaaccgagca cggacctggg tgcctggctg
     4381 cgtgggattt tttgaaatcc aaagcgacgg aaaaaggtct cagccgtggt tgtcagcaac
     4441 acaatctttt tcaaccgctg ctgcgcagcc tgatcaagca acctgaggac aagctgtttc
     4501 ccaattccgc tgttgcgctc tgactctgca acggctactg aacgcaacag cgcgtctttt
     4561 ccgtagcgct ccagcccggc acacccaacc agctttccgt ttcgagacac actggtgaat
     4621 tcgagattcg gtgactcaat tccatccaca ggcagacgcg aagctatcaa caggcttcga
     4681 ataaactcaa gttgatcaga tccaactggc tggaagctca acccagaatc aaggtgcgtg
     4741 tcagatacac caccgacaac cgaaccggaa agctcggtcc gcaacgcgtc tcgaatcagg
     4801 cgaaggactt caaaaatctg aggttgctgg atggcatcaa ttcgattgag gacccgttct
     4861 gcctgagtgt cgagcacagc gttgaggttt tcaaatcgct ggtgtccagc ttcggtcaag
     4921 gaaaggtgaa ccaggcggcg atcttcatca ctctgaactt tggcaatcaa accttcctgc
     4981 accagcgatt caacggcccg actggtccag cttttatcca gcccgacccg gcgggcaagg
     5041 tccgccagtg tcagagttcc agttcgtccg agttcactga tgataaaaca ttgggttgat
     5101 gtcgtctggt cacaactggc cactgccagt cgttgtaatt ggaggtgcaa tcgggcggtt
     5161 tcgcgcaaga tcgccccgat acgttcaatg tcttttgcga actccatact caggttgagg
     5221 ctccagtacc gtttctttgt gagggatgcg tggcagaaaa tcatccctgc cagtctggca
     5281 ggttggcatt tcaaaatatc attgtcaata gcaactaatc cgcaaaggag caaaatagca
     5341 aagttgtgaa ataaccagat agcctgccgt tcattctcag aagcacctgg ccaacctctc
     5401 aacgctgtat ttccacaatt tcacgatttc gcgacttcac tattttgcta tttcgctatt
     5461 tccacaggcg tgttttcacg tgcaatgatc tcagcgacgg ctgtgcgcaa acgctctaaa
     5521 attccttcat tggtggtgcc gcggatatca accgggtaaa aatgagcttc attaaatccg
     5581 gtgtggcgaa taatccgctt aaacaacttt gattgcacct ctggagcaca ggcacctgac
     5641 agaatatagg tgtctgggcc tgccgattgg aaaatatcca tcaacaccgc atttccaccc
     5701 tgaccacaca attgtgggtt gaccaggctg tacgtgacat ccaaacctga ctcaatttgg
     5761 aatggaagcg tgttcatgtc agcgtcttta aacgacgtac acatgccctt acaattgcaa
     5821 agtacaactt tgatttccat actgcctccg tgggaaccaa accagaaatg tcttgacccc
     5881 tgatttgaaa gaggcaattc acgtacccgg aaatcaaagt aatcaccaaa ttcaggaatt
     5941 gcgaaaacat cttattttac aatcatttgg caaccattcg agtttcattc actgtttgcc
     6001 acaatacgcc tgtaccttga aaccaaaaga tttcacctga aatctggtta ccacttgaac
     6061 caaattgcgg attattcccc agattgttcg tgagcacctg tccaaggctc agtcagtgca
     6121 ggtaaaacag caaccgggtc gcaggtgaaa ggcgaaactt tcagtcagtt acggtatatg
     6181 ccgagacagg aggagcgaaa agaagccggg ttgacgactc gcgacgtcgc ttttagtaac
     6241 tccgggtaag tccgccatcc acagtaacgc tttgccccgt gatgtagcca gcttcctcag
     6301 ataataaaaa ggccacagtc cgggcgattt cctgaagctg tgccgggcgg ttcatcggaa
     6361 ttgcctctcg cgtttcgtct gaaatttcat aattttcgac atatccaggc aagatgcagt
     6421 tcatacggat attggcgcgg gcatagcggt cagcatagag cttggtaaaa ctcgacagtg
     6481 ctgcccgcaa caccgatgac accggcattg gcagtgacgg catgcgcatg gtgaatgagg
     6541 aaatattgac caatccgcca ccgccctgca gttccatcac cggcgtgacc agccgtgaca
     6601 ttcgcaccac atttaagagc aagagatcca accctttgtg ccagtcctca tcctggatgt
     6661 tgagcaactc gcccttggcc gggtggccgg tgttgttcac tacggcatca attcggccat
     6721 atttttcaag ggtggcggta accagacgtc gaatgtcggt ttcttcggtc accgaaccgg
     6781 taataccaaa accacccagt tctgaggcca ggtcttcagc ttcggtcgaa cgtgacatga
     6841 gtgccagttt atagccacgg ttggccagtt cacgggcaca ggcggcgccc attccacgac
     6901 tggcagccgt gataatggca actttaggtt cctgcatcgt gtgtgttcct ttgtgatttg
     6961 agtgaaggtt gaaggcattg acgcagatgc tgcaacacct ggatggctgt ctcaacctcg
     7021 gtgacctcaa ggtcgtgcga cagcctggaa atttcagttg aaatcgtggt ggaaagttct
     7081 tgaaagacgg cttctccgtg ggcagtcaac tgtacgagtt ttgatcgctg gtggtcagga
     7141 ttggcaacaa attcaatcac tccgtcctga gcgagttcat tcgccagcgt ctgcatccgt
     7201 tgacgggtga ccgagcgcat gcgcgccagt tgtggaaccg tccgtggacc agtagttttc
     7261 aacaatcgca acattcccca atatccgcca cctgactgcg ttaccgcgcc aatgcgtttt
     7321 cctgccgccc ggagggtaaa aaacatctcc agcgtttcaa ccagtaacgc ttcaaccgcc
     7381 tgattcaata tttgtgcagc cggcacctga tttggttggg ggtccatagt cagcatccat
     7441 ccatgaaaaa atcgctattt gtctgggttt caacacgttt gtcaagccag gaatccttct
     7501 gaatgagaaa actggtcgtg agtgcggttt tatattgaca ataaaattgt cattatgaca
     7561 agtttattgt caatactgac agttttacca atggaggaat tcgggcatgg gctacattgc
     7621 gtctcaattt ggtaacccac gtgggctggt tggaaaatgg attggctggt tgatggcaat
     7681 caaaaacaat gaccgaattg actggacctg ttcacaactt ggagttcgtc cgggagatcg
     7741 cctgattgaa attgggtatg gtccgggagt tgctattgaa aaacttatcc aaagcacacc
     7801 acagattcaa attgtgggca ttgatcactc tgaaaccatg tttgcgcagg cttaccggcg
     7861 caacctcaaa gtgattcaaa cgggtcaggt cacattgcac gttggaactg tggcgccgct
     7921 tccattggcg gattgttctt ttcacaaagc acttactatc aattccagtt ttttttggcc
     7981 cgacccggtc gaaggttttc gcgaactgct ccgagtgttg aaaccagaag ggaaaatcgc
     8041 cgtgaccgtt cagccttttt ttgccaaaac tgatgatcag tttcagaaag cgggaacgac
     8101 tctggtcagt cagttgcggg aagccggttt ccagctcatt tcacttgacc gaaaacacat
     8161 gaggccggtt gattgctggt gtgcaattgg gctgaaaaag cgaattgaac catctatttt
     8221 ttgacaggtg tctgaaaatc agatacctat gctgcttttt aagacggttc ctttgttttc
     8281 cgtgaggtct tatgaaaatt tttgtcaccg gcggcgccgg atttattggc tcacacgtag
     8341 ccgacgcatt tctgaacctt ggtcataaag tcgtcatttt cgacaatctc tcgactggac
     8401 gacgtgaaaa tcttaatcct caagccaaat ttatcgaagg tgatctgggt gatgattcgt
     8461 tgcctgacat tctgaaggct gaatctccgg atgtgatcag ccaccatgct gcccagattg
     8521 atgttcgcaa atcggtcacc gacccgcgat ttgacgccca ggtcaatatt ttgggttcca
     8581 ttcacctgct cgaagctgcc cgtcaggctg gtgtcaaaca cgtcatgttt gcttcgacgg
     8641 gcggtgcaat ttacggagag caggactact ttcccgccga tgaaaaccat ccgttgcggc
     8701 cctgttcgcc ctatggcatt gccaaactgt cggttgaaaa gtacctggat tattaccggc
     8761 tggtgtatgg tctgaagacg accatcttgc gctatgccaa catttacggc ccgcgccaga
     8821 acccgcatgg cgaagccgga gtggtcgcca ttttctgtga acggatgctt tccggcaaac
     8881 cacttgtgat caatggcgaa gggttgcaaa cccgcgatta tgtgtttgta ggcgatgtgg
     8941 tcaaagccaa tctgttcacg atcaatgatg accacagcga tatttacaac gtcggcacgg
     9001 gtgttgaaac cacggtggtg gatatctttc atcacctgcg tgaagcatcc gggttggaca
     9061 ttgccgaaaa ccacggtcca gccaaagctg gcgaacaact ccggagcgtg ctcacctcgc
     9121 gcaaactctt tgaacacttt ggctggaaac cccaaatgcc gattgccgaa gggctaaaac
     9181 tcacgttgga aagctttcgc caaccggtac agtaatcact ccgattttaa tgcaatttgg
     9241 aaaagtctta tgcaaaacct gcttgtgact ggcggatgcg gcttcatcgg aagcaatttt
     9301 attcggtatc tgcttgaaga atctgatttt accgggcgtg tgatcaatct tgacgcgctc
     9361 acctatgccg gaaatccgga aaatctggct gatctggccg cccgctctcc gcaacgttat
     9421 acatttgtcc aggctgacat ttgtgatgcc gccaaagtgg ctgaaatctt tgacagtcac
     9481 cagattgaca cggtctgtca ctttgccgct gaatcacacg ttgaccgctc gattgtcaat
     9541 ccgggggcgt ttatccagac caatgtcgtt ggcacctaca cactgcttga agccgcccgt
     9601 cagcgcaaag atcgaatttc actctttcac catgtcagca ctgacgaagt ctatggcagt
     9661 ctgggcgagg aagggttctt caccgaggac acgccatacc gtccaaccag cccctattca
     9721 gcttcaaaag ccggttcaga ccatctggtg cgggcctatt tcaagacata tgggttgccg
     9781 gtgacaattt cgaactgctc caacaattac ggcccctatc atttcccgga aaaactgatt
     9841 ccgctgatga ttcttaatgc gctcgaaggc aaaccattgc cggtgtatgg cgatggtcga
     9901 aatgtgcgcg actggctcta tgtccgcgat cactgcacgg caatctggac ggtgatgaat
     9961 cgagggcaac gcggacagac ctacaatatc ggcggccaca atgaaatccg aaacctgacc
    10021 gtagtcgaga ccatctgcac attgctggat gaaatggttg agccgttgcc cactggaccg
    10081 cgctccaatt taattacctt tgtcaaagac cggcccggtc acgactggcg ttacgcgatt
    10141 gattcaacca aaattcaaca ggaattaagc tggacaccgg aagaatcatt cgaaaccggc
    10201 atccgcaaaa ccatcacgtg gtatttggaa aaccgcacct gggttgacca cgtccgaagc
    10261 ggcgaatatc aaacctggat ggcggcccag tatggagtga ctgggtgatg tgagaatgca
    10321 gaatgcagaa cccttttagc agttagtggt tggtttttag tgcctggttc ttggttcttg
    10381 gtgcttggtg cttggttctt ggttcttggt tcttggttct tggttctttg aaagtattgg
    10441 ttcccaggca ccaaacacta aggaccaggc actaaccact tgtattttct tcattcttca
    10501 ttcttcattc ttcattctca attcttcatt cttcattctt c
//
LOCUS       DAOQDO010000005         9989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3439_length_9989_cov_9.029897, whole genome shotgun sequence.
ACCESSION   DAOQDO010000005 DAOQDO010000000
VERSION     DAOQDO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_9989_cov_9.029897"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /gene="lon"
                     /locus_tag="PKZ53_00245"
     CDS             <1..1732
                     /gene="lon"
                     /locus_tag="PKZ53_00245"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNJ38889.1"
                     /translation="ELLAREISVLQVQQEINSQAKGEIDRSQREYYLRQQLKAIQQEL
                     GEGNELLEEINQYREKIAEVKMPEKVEEEVLRQLKKLERMHPDAAEASTLRNYLDLMV
                     GLPWSVSSTDNLDLKQAQTILDEDHYGLEKVKERIIEDLAVRKLKGKTKGALLCLVGP
                     PGVGKTSLGRSVARALGRKFIRISLGGIHDEAEIRGHRRTYVGAMPGRIIQAIQQATT
                     NNPIIMLDEIDKVSSDFRGDPSSALLEVLDPEQNHSFRDNYLGVPFDLSNAMFMMTAN
                     ILDTIQPALRDRMEVLRLSGYTEEEKLAILRRHIIRKQMDENGISSKHISFSDSALRA
                     IIMQYTRESGLRQLEREIASVCRKVARRVAEGQTGRVQVTVKNLQEFLGVSKFMADEL
                     LKKDQVGVATGLAWTPVGGDVLYIEVLLMKGKGSLLLTGKLGDVMKESAQAALSFAKA
                     RCKELGILESDFAAHDIHIHIPEGAIPKDGPSAGITLATAMVSAFSNKPIRKSVAMTG
                     EITLRGNVLPIGGVKEKVLAARRAKITTVILPQQNRKDVEEMPKELVKDMEFVYVEHV
                     RQVLKTALVS"
     gene            1779..2369
                     /locus_tag="PKZ53_00250"
     CDS             1779..2369
                     /locus_tag="PKZ53_00250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ38890.1"
                     /translation="MHSLAFILLSVLSLLLPVIPQESHEYAPLQEQKIAYKDWTYKSL
                     KADAPSVNLRDWATGKKLVLVVYYAPWCGNWHNEAPVVARLYDKYHAQGLEVIAVNEY
                     GSSDEARTFFAGNKLSIPVVVESEDRADREKTTHFGYRQTTGDTRRWGSPYNVFLEPV
                     TFPKQGDVLAEKAWVVNGELIEAEAEKHIRERLGIQ"
     gene            2469..2756
                     /locus_tag="PKZ53_00255"
     CDS             2469..2756
                     /locus_tag="PKZ53_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38891.1"
                     /translation="MAITEQQAVEIAVADFARRRGVNRSDIKVTRTEKTQFANAVLGA
                     PVSGEMAAMMMTDGWRIIVQTKGDGDDAEYRANSQQVRLFRYQGSHYKIYP"
     gene            2821..4233
                     /locus_tag="PKZ53_00260"
     CDS             2821..4233
                     /locus_tag="PKZ53_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ38892.1"
                     /translation="MKRSPLFHLTLGLMVVIAAYWAGAIHRWETQRRALPKQLCSGIP
                     SLKQSDFPAGHSCGKTAGLEYRGYIVKYKQGTKRSAIEALLARLKGEHINEYKYLPGL
                     SFETVGASLSVEDVLRIYRADPNVEFVEPNYVYHQSDSWPNDPGFEQQWGHHNTGQPI
                     QGRPGGKAGADISAIAAWNISTGSKDIVVGVVDSGIDYLHPDLAANMWVNENEIPDNR
                     IDDDKNGVIDDVYGYNAITNSGDPMDDNNHGTHCAGIIGAVGDNGEGIVGVNWNVRIM
                     ALKFLSGHGGGSLNDALECINYGLEMKARGVNLRVLSNSWGGGGYSKALEEAIQRAND
                     QGILFVAAAGNDETNTDKDPNYPSCYNVPNVISVAALNNNDDLAVFSNYGENTVHIGA
                     PGVEVYSTIPNGQYAYYSGTSMAAPYVSGAAALVLSKTPDLKAADLKARLLDSAVPVK
                     SLKGKIKTGGRLNANQALVE"
     gene            complement(4367..7750)
                     /locus_tag="PKZ53_00265"
     CDS             complement(4367..7750)
                     /locus_tag="PKZ53_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ38893.1"
                     /translation="MRLQFSSDFRKIFGEYLPAQMIDVQILRHALETVRTCHTQWGLS
                     PPCFWIASPPDHWEKWTRTLVEFGLAQSHELLFTATQKVHPFWVYLFLDAQFSCCIAA
                     ESLPSTGLDQGPTLVGSISFDPHLVEKTAHTLLRHQSQKIQRLLRDALHNPHLDKVTR
                     QIHRAEATERFASLLAQTQVQQAAAPTSISATVSLSYLEELIGILSDSASGQAGAALL
                     HRVVNAIAHLTDYRLVLVSLFKEEFPYRDLVGCDAIPAEILERIHQIPFPQDEFRQLL
                     HLGEPIQVGTLGRALYFPPRLHHVLERENLYKSPEPYTGGQGKWNKHDELFVPIVSQD
                     GEYLGVMSLDDPRSGKSPTEQSVLPVIAFARQISLILERHRVEQEMERMLIQLSVNNS
                     ELAGLNLVLETTSRSMDRHGVLEGALKILDVVDLEAAAILTLDENREQLRLEHVSNVP
                     DPLCRLMYDFGVECFTVGLKTTRDELEIHDLETIHTSLPHLKQTLHREQIQTLVSVPL
                     SGRNGVLGVMILWSHEKRSFSAPDCRLLANVGKQVGLALETTQLFEHSHRLATQMSAL
                     FEVGKSITSHLALKPLLDSVTQNAATLLNADQVSVALLDDSHAVTELTKWIRPVPPGL
                     TVINSDTPPGTPPPTDKTPPNGILLSLPIRTTHPETGEARQIGMFHVGRNSPRQPFSQ
                     VEVEMLNNLTTHVSIAVENARAFEAEHKRSKQFRLINTFLREISAILDSEEMLKRAAQ
                     VICENFGYVQVSIHLYHAEAQLVMVRAVVGNQVAVGWNPGDVLPCTQGILHQAIQTRH
                     TVKIDDVRKVKDELVSIPEVRSRLAIPVLRGSEVIGILLLEHSDPGHFDRSDVITAEI
                     LADGIGVALRKTELYSEVMLANTQLQEMIRVKDDLVSMVAHDFRSPLTTIQAYSEVLS
                     DQTEDSSTKRYLQIINQQSRHLARLANDTLTMSRLESHKMNFEFQPVYLRELIELVLD
                     SRFGETNITFETVWPETPIVVSADSGRLQEVFDNLIGNAVKYSPAGGSVVIKGSFQGD
                     IACLSITDHGIGIAKEDLPKLFERFQRMGNARKLRIPGTGLGLYICRSIIEAHNGHIE
                     AESELGQGSTFRVFIPLHQPDKTETPSENERRTETIDSP"
     gene            complement(7907..9388)
                     /locus_tag="PKZ53_00270"
     CDS             complement(7907..9388)
                     /locus_tag="PKZ53_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ38894.1"
                     /translation="MSPSIKSPSDTATSAGRGAIYITLAKFYFIFTGYAIIFALPRLL
                     TKEQYGEYVVVTSFVSIINAVIITGTQQAVSKFVSENPASAESLRSRSLAVQAILGTL
                     IVMAYVLLTPYLATQFWNDASLITPLRVSALITFCYAFYAVYMGYLNGKKEFFWQATL
                     DSSYSTFKMLAIIACALVFRSVTGAVGGFALGAFLVLILAIGLAGRGRSTIAPHIELG
                     TYLTFQLPLLAFVLCNNLLQKVDITFLKAFSSVDPAIASANAGDYGALINIANITYQA
                     VISVTFVVFPLISKASFEKETGEVKTYIRQTMRVALLVMALLATIFSSNAADTLGLLY
                     PKIYLSGAGALQIVAFGMLSFGLISVLTTIITGSGHPWISLFIGAFTLMANVVLNYKL
                     IPVYGLRGAAIGTTISMTVGALLACGYVLFSFKALISPLTLGRIVLASVLIYGLHLIP
                     LAGVLVVVKMTIQGLAYLTILILTRELGAQELRLIQKIFRLAK"
     gene            complement(9576..>9989)
                     /locus_tag="PKZ53_00275"
     CDS             complement(9576..>9989)
                     /locus_tag="PKZ53_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ38895.1"
                     /translation="NRILFATDFSADDAAAKTLAINLAREHHSRLIFTHALGVFLNYV
                     RAVTTIGTDDIESHVRKEATEKLAALQAEAEGIESKTVLGEGRTYSEVLRIAKESDVD
                     LIVVGTGNRQTGNVLGRNTERIMRDATCPVLIVPS"
BASE COUNT         2572 a         2441 c         2655 g         2321 t
ORIGIN      
        1 tgaactcctg gcccgtgaaa tcagcgtgtt acaggtccag caggaaatta acagccaggc
       61 caaaggagaa atagaccggt ctcaacgcga gtactatctg cgccagcaac tcaaagccat
      121 tcaacaggag ttgggtgaag gcaatgaact gctggaagaa atcaatcagt accgcgaaaa
      181 aattgctgaa gtcaaaatgc cggaaaaagt cgaagaggaa gtcctgcggc aattgaaaaa
      241 gctggaacga atgcacccgg acgccgcaga agcttcgacc cttcgcaatt acctggactt
      301 gatggtgggg ctgccgtggt cagtttcctc aaccgacaac ctggacctca aacaggcaca
      361 aacgattctg gatgaagatc attacgggtt agagaaagtg aaagaacgga tcatcgaaga
      421 tctggctgtc cggaaactca aaggcaaaac caaaggggcg cttctgtgtc tggtcggtcc
      481 tcctggcgtt ggaaagacct cgcttgggcg gtcggtggca cgtgcgctgg gtcgcaagtt
      541 tattcgcatt tcacttggcg ggattcatga cgaagctgaa attcgcggcc atcgacggac
      601 ttatgtcggt gccatgcccg ggcggattat tcaggcaatt caacaggcga cgaccaacaa
      661 tccgatcatc atgctggatg agattgacaa ggtgagttca gattttcgcg gcgacccaag
      721 ctcggcgctg ctcgaagtgc ttgacccgga acaaaaccat tcgtttcgtg acaactatct
      781 tggcgtaccg ttcgatttgt cgaacgcgat gtttatgatg acagccaata ttctggacac
      841 gattcaaccc gccttgcgcg accgcatgga agtcttgcga ctgtcggggt ataccgaaga
      901 agaaaaactc gccatcctgc gccgtcatat catccgcaaa cagatggatg aaaacgggat
      961 atcatccaaa cacatttcat tttcagattc agccttacgg gcaatcatta tgcagtacac
     1021 acgtgagtcc ggattgcggc aactggaacg tgagattgct tcggtctgtc ggaaagtggc
     1081 ccggcgggtg gctgaaggcc aaaccggacg cgttcaggtc acagttaaaa acttgcagga
     1141 atttctgggt gtttccaagt tcatggctga tgaactgctc aaaaaagacc aggttggtgt
     1201 ggcaactggt ctggcctgga ctccggttgg tggtgatgtc ctctatatcg aagttttatt
     1261 gatgaaagga aagggcagcc tgctcttaac gggtaaactg ggtgatgtga tgaaggaatc
     1321 agcccaggcc gcactttcgt ttgccaaagc ccggtgcaaa gaactgggaa tcctggaatc
     1381 tgattttgcc gcccacgaca tccacattca tattcctgaa ggcgctattc cgaaagatgg
     1441 tccttccgcc gggattaccc tggccacggc catggtttca gctttttcca acaaacccat
     1501 tcgaaaatca gtcgcgatga ccggagaaat tacacttcgt ggaaacgttc tgccgattgg
     1561 tggtgtcaaa gaaaaagtac tcgctgcccg acgtgccaaa attacaactg tcattttgcc
     1621 ccaacaaaac cgcaaagacg tcgaagaaat gcccaaagag ctggtcaagg acatggaatt
     1681 tgtctatgtc gagcacgtcc gacaggtttt gaaaacagcc ctcgtcagtt aggtcagtac
     1741 cacctgcgtt agcgggtggg tctttcggag gaaatgaaat gcactcactt gcctttatac
     1801 ttctcagtgt attgagtttg ttgttgcctg tgatacctca ggaatcacat gaatatgcac
     1861 cactccagga gcaaaaaatt gcctataagg attggaccta taagagttta aaggcagatg
     1921 ccccgtccgt gaatttgcgt gattgggcaa cagggaaaaa gctggtgctg gtggtgtatt
     1981 atgcgccctg gtgtggcaac tggcacaacg aagccccggt tgtcgctcga ctctatgaca
     2041 aataccatgc tcaaggtctg gaagtgattg cggtcaatga atacggttcc agtgacgaag
     2101 cccggacctt ctttgctggc aacaaactct cgattccagt ggtggttgaa tcagaagacc
     2161 gggctgaccg cgagaaaacc acgcactttg ggtatcgcca gacaaccggt gatactcgcc
     2221 ggtgggggtc gccctacaat gtttttctcg aacccgtaac atttcccaaa caaggtgatg
     2281 tcctggcaga aaaagcgtgg gtcgtcaatg gtgaattgat tgaagccgaa gcggaaaaac
     2341 acattcgcga acggcttggg attcaatgac ctgtgtgctg gcttgtcttc tcacccgagt
     2401 cgggctagag ttggacattt cccttctcca aactggaaac ttcctcaaaa ggacgagtgc
     2461 caaaaacgat ggctattaca gagcaacagg ctgtggaaat tgctgtcgct gattttgcca
     2521 ggcgacgagg cgtcaaccga agtgatatca aggtcacccg caccgaaaaa acccaatttg
     2581 ccaatgcggt cttgggggcg ccggtcagcg gtgaaatggc agccatgatg atgactgacg
     2641 gctggcgcat aattgttcaa accaaaggcg acggagatga tgccgaatat cgtgccaact
     2701 cgcagcaggt tcgtctcttc cgctatcaag gctcacacta caagatttat ccgtgacgtt
     2761 tggccaaact gattttgacc aggcgccacc caacgttgtg acatcaggag gtacgaggcc
     2821 gtgaaacgct caccactttt ccatctcaca ctcgggttaa tggtggtcat tgctgcttac
     2881 tgggcagggg ccattcatcg gtgggaaact caacgtcgag ctttacccaa acagctctgc
     2941 agtggaattc cttccctgaa acagtctgac tttccggctg gtcattcgtg cggcaaaacg
     3001 gctgggctgg agtaccgtgg ctatattgtc aagtataaac aaggcacaaa acgcagtgcg
     3061 attgaagcct tgctagcccg gctgaaaggc gagcacatca acgaatacaa ataccttcct
     3121 ggtctctcat ttgaaacggt tggcgcttcg ctcagtgttg aagatgtgct gcggatctat
     3181 cgtgctgatc ccaatgttga gtttgttgag ccaaactatg tgtatcatca atcagatagc
     3241 tggcccaatg atcctgggtt tgaacagcaa tggggacacc acaacaccgg acagccaatc
     3301 cagggccgac cgggtgggaa agccggggct gatatttcag ccattgcggc ctggaatatt
     3361 tcaactggca gcaaagacat tgtagttggc gtggttgata gtgggattga ttatctgcat
     3421 cccgatctgg cggccaacat gtgggtcaat gaaaacgaaa ttcctgacaa ccgcattgat
     3481 gatgacaaaa acggcgtgat tgatgacgtc tatgggtaca atgccatcac caacagtggg
     3541 gatccgatgg acgacaacaa ccacggaaca cattgtgctg gaatcattgg tgcggttggg
     3601 gacaatggcg aaggcatcgt cggcgtgaac tggaatgtcc ggattatggc gctcaagttc
     3661 ctgtcaggtc acggcggtgg ttcgctcaac gatgccctgg aatgtattaa ctacggcctg
     3721 gaaatgaagg cccgaggcgt gaatttgcgg gtgctttcca atagctgggg tggtggtgga
     3781 tattcaaagg cattggaaga agcgattcag cgagccaatg atcaaggcat tctgtttgtc
     3841 gctgccgccg ggaacgacga aaccaacacc gataaggatc ccaattaccc atcctgttac
     3901 aacgttccaa atgtgatttc ggtcgcggcc ctcaacaaca atgatgatct ggccgtgttt
     3961 tcaaattatg gggaaaacac agttcacatt ggggctccag gcgtcgaagt ctacagcacg
     4021 attccaaatg gccagtatgc ctactattca ggcacctcga tggccgcccc ctatgtttca
     4081 ggcgccgcag cactggtgct ctcaaaaaca cctgacctga aagcggctga cttaaaagcg
     4141 cgattgcttg acagtgcggt tccggtcaaa tcactgaagg gaaagatcaa aactggtggc
     4201 cggttaaatg ccaatcaggc gctggtggag tgatctttat cgggttatcg ggttccgggt
     4261 tccgggttcc gggttccggg ttccgggttc cgggttccgg gttccgggtt ccgggttccg
     4321 ggttttcgaa tctgaaggtt ctctactcaa ggctctccgt tctatgctaa ggcgaatcga
     4381 tggtttcggt tcgcctttcg ttttctgatg gggtttcagt tttgtctggt tggtggagcg
     4441 ggataaaaac acggaaggtc gagccctggc ccagttcgct ttcggcttca atgtgcccgt
     4501 tgtgggcttc aatgattgat cggcagatat aaaggcccag tccagtacca ggaatccgca
     4561 gtttccgggc attgcccatt cgctgaaatc gctcaaagag tttgggaagg tcttctttgg
     4621 caatcccaat cccatgatca gtgattgaga ggcaggcgat gtctccttga aatgatcctt
     4681 tgatcacgac gcttccacca gcgggagagt atttcaccgc gttgccgatc aagttatcaa
     4741 agacttcctg aagtcgtcct gaatcggctg acaccacaat cggggtttcc ggccagactg
     4801 tctcaaaggt aatgttggtt tcaccaaacc ggctatcaag aaccagttca atcagttccc
     4861 tcaaatatac cggttgaaat tcaaaattca ttttgtgtga ttcgagccgt gacattgtca
     4921 gcgtgtcatt ggccagccgt gctaaatgtc gggattgctg attgataatt tgcagatacc
     4981 gtttggtgct cgaatcttcg gtttgatcag acaggacctc gctataggct tgaatagtgg
     5041 tgagcggcga gcggaaatca tgggcgacca tcgaaaccag atcatccttg acccgaatca
     5101 tttcctggag ctgggtgttg gccagcatga cctcgctgta gagttcggtt ttacgcagtg
     5161 cgacgccgat tccatcagcc agaatttcag ccgtaatcac atcactccga tcaaaatgcc
     5221 ctgggtcaga atgttcaagc agtaagatgc cgatgacttc actgccgcgc agcaccggaa
     5281 tggcaagtcg tgagcggact tcggggatac tgaccagttc atctttcact tttcgcacat
     5341 catcaatttt gaccgtgtgt ctggtttgga tggcctgatg caaaataccc tgggtgcaag
     5401 gcaatacatc ccccggattc catccgactg ccacctgatt gccaaccacg gctcgaacca
     5461 tcaccagttg ggcttcggcg tgatagaggt gaattgagac ctgcacatac ccgaaatttt
     5521 cacaaatgac ctgagccgcc cgtttcagca tttcttcaga atcgagaatg gcgctgattt
     5581 cccgcaaaaa cgtgttgatc aaccgaaatt gcttgctgcg tttgtgttca gcctcaaatg
     5641 cccgtgcatt ctcaaccgcg attgagacat gggtggtgag gttgttgagc atttcaactt
     5701 caacctgaga aaaaggctgc cggggcgaat ttcgaccaac gtgaaacata ccaatttgcc
     5761 gtgcttcacc ggtttcagga tgcgtggtgc gaattggcag gctgagcagg attccatttg
     5821 gaggtgtttt atctgttggc ggtggtgtgc ccggaggggt atcagagtta ataaccgtga
     5881 gtcctggcgg aaccgggcga atccatttgg tcaattccgt cacggcgtgc gaatcgtcca
     5941 ggagcgccac ggaaacctga tccgcattta acagcgtggc tgcattttgg gtgactgagt
     6001 ccaggagcgg tttgagtgcc agatgtgaag tgatgctttt accaacttca aacaaggcac
     6061 tcatttgggt tgccagtcgg tgcgaatgct caaacaactg cgttgtttca agcgccagcc
     6121 ccacctgttt tccaacgttg gccagtaagc gacaatccgg ggcggagaaa ctccgttttt
     6181 cgtgcgacca taagatcatc acccccagta ccccgttgcg gcctgacagg ggaacgctga
     6241 ccaacgtttg aatttgttct cggtgcagcg tttgcttgag atgcggaagc gatgtatgga
     6301 tggtttcgag atcgtggatt tccagctcat cccgagtggt tttcagtccg accgtaaagc
     6361 attctacccc aaagtcatac atcaggcggc agagcggatc cggcacattg ctgacgtgtt
     6421 cgagtcgaag ttgttcgcgg ttttcatcca gcgtcagaat cgcagccgct tcgaggtcaa
     6481 ccacatcgag gattttgagc gccccttcca gcacgccgtg ccggtccata gagcgactgg
     6541 tggtttcaag taccagattc aacccggcca gttcgctatt attgaccgaa agctgaatca
     6601 gcatgcgttc catttcctgc tcaacccggt ggcgctctaa aatcagagaa atttgtcggg
     6661 caaaggcaat cactggtaac acgctttgtt cggtcgggga tttgccagat cgcggatcat
     6721 caagcgacat cacccccaga tattcgccat cctgactgac aatcgggaca aacagttcgt
     6781 cgtgtttatt ccactttcct tgccctccgg tataaggctc agggctttta tagagatttt
     6841 cacgctctaa cacatgatgg agccgtggcg gaaagtagag cgcccggcca agcgtgccaa
     6901 cctggattgg ctcccctaga tgaagcaact gtcggaattc gtcctgtgga aatggaatct
     6961 ggtggatacg ctctaaaatt tcagccggaa tggcgtcaca gcccaccaaa tcacggtagg
     7021 gaaactcttc tttaaacaac gagaccagca ccagccggta atctgtcagg tgggcaatgg
     7081 cattgaccac tcgatgaagc aatgccgcac cggcctgacc cgatgccgaa tcgctcaaaa
     7141 taccaatcaa ttcttcaagg tacgaaaggg aaaccgtggc tgaaatactg gttggcgccg
     7201 ctgcctgttg aacctgggtt tgggccagca atgaggcaaa tcgttcggta gcttcggccc
     7261 ggtgaatctg acgtgtgact ttatcaaggt gggggttatg taaggcatca cgcagcaggc
     7321 gctggatttt ctgagattga tggcgcagaa gggtgtgagc tgtcttttca accagatgtg
     7381 gatcaaaact gatacttccc accagcgttg gtccttgatc aagccctgta gatggaaggc
     7441 tttccgctgc gatacaacag gaaaactgag cgtccagaaa aagatacacc caaaatgggt
     7501 gtactttttg agttgccgta aacagcagtt cgtgactttg ggccagtcca aattcaacga
     7561 gcgttcgagt ccacttttcc caatggtcag gaggcgaggc aatccaaaag caaggtggtg
     7621 acagccccca ctgagtatga caggtccgaa cggtctccag tgcgtggcgc agtatctgca
     7681 catcaatcat ttgggcaggg agatactcgc cgaaaatttt tcggaaatcc gaggagaact
     7741 gaagcctcat gaaacttggt ccagaatgtg gaatgagatg atttgtgctg atagaaattg
     7801 gtaaccagga ttgccgccca gtatacacca attacccaaa tgacaaagtg acaaggtgac
     7861 aaggtgacat ttgatcaagt ggcaagattg aaaataaaca aatctgtcat ttggccaggc
     7921 ggaagatttt ttgaatcaac cgaagttcct gggcgccaag ttctcgggta agaatcagaa
     7981 tcgtcaggta cgccagccct tgaattgtca ttttcacaac caccagaact ccagccagtg
     8041 gaatgagatg gagtccataa atcaagactg atgccagcac aatccgtccc agggtcagag
     8101 gggaaatgag ggctttgaag gagaagagaa cgtacccaca ggccagaagg gcaccaaccg
     8161 tcatggaaat ggtcgttccg attgccgcac cccgcaagcc atagactgga atcaatttgt
     8221 agttcagtac cacattggcc atgagggtga atgccccaat gaagagcgaa atccagggat
     8281 ggccgctccc tgtgataatc gtggtcaaca ctgaaatcaa cccgaaactc aacattccaa
     8341 acgccacgat ttgcagtgct ccagccccgc tgaggtaaat tttaggatag agcaacccca
     8401 atgtatctgc cgcattggac gaaaaaatcg ttgccagcaa tgccatcacc agcaatgcca
     8461 cccgcatggt ctggcgaatg taggttttga cttctccggt ttctttttca aaagaggctt
     8521 tggaaatgag cggaaagacc acaaacgtga ctgaaatcac tgcctgatag gtgatgttgg
     8581 caatgttgat caatgcccca taatcacccg cattggcact ggcaatggcc ggatcaaccg
     8641 aggaaaacgc tttcaggaag gtgatatcca ctttttgcaa caggttgttg cacaaaacaa
     8701 atgccagcaa cggcaactga aatgtcaggt aggtccccaa ttcaatatgg ggagcaatcg
     8761 ttgatcgtcc acgtccagcc agcccaatgg ccaaaatcaa aacgagaaaa gcgcccagtg
     8821 caaaaccacc cacggcgcca gtcacactcc gaaagaccag cgcacaggca ataatggcca
     8881 gcattttgaa cgtcgagtag gaggaatcga gcgttgcctg ccagaaaaac tctttctttc
     8941 cattgagata ccccatgtaa accgcataaa aggcataaca aaaggtgatc agcgctgaaa
     9001 ctcgcaatgg ggtgatgagt gaggcatcat tccaaaactg agtcgcaaga tacggcgtca
     9061 acagcacata ggccatcacg ataagggttc caaggattgc ctggaccgct aacgaccgtg
     9121 acctgaggct ttctgctgag gccggatttt ctgaaacaaa ctttgaaacc gcctgctggg
     9181 taccggtaat gatgacagca ttgatgattg agacaaagga cgtcaccaca acatactcgc
     9241 cgtattgctc cttggtcaaa aggcggggca aagcaaagat gatcgcatat ccggtaaaga
     9301 tgaaatagaa cttggccagc gtgatataga tcgcaccacg cccagccgaa gtagctgtat
     9361 cagatggcga tttgatggat ggtgacaagg tcgtaggatc cgaggtgggg taagccgagg
     9421 ggttagctgg agtactcatg aggaagaatc gcgaggagca aatcgcgagg agcgaggagc
     9481 gaggagtttc aaattcaacg ggatacaatc gttaacaaac agagcaatcc agatatattt
     9541 gactccttgc tcctcgccac tcgctaccaa aagatttagc ttggcacaat gagcaccggg
     9601 caggtggcat cacgcataat gcgttcggta ttgcgcccaa ggacgttgcc ggtttgacgg
     9661 tttccagtgc caaccacaat caggtcaaca tctgattctt tggcaatccg caatacttca
     9721 gagtacgttc gaccttcacc caaaaccgtt ttggattcaa tcccttcggc ttcagcctga
     9781 agtgccgcca gtttctcggt ggcttctttg cggacgtgag attcaatgtc atcggtgcca
     9841 atcgtggtga cggcccggac ataattgaga aaaacgccca gggcatgggt aaagatcagg
     9901 cggctatggt gttcgcgggc caggttgatg gccagggttt tggccgccgc atcatcggct
     9961 gagaagtcgg tggcaaaaag aattcgatt
//
LOCUS       DAOQDO010000006         9635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3672_length_9635_cov_3.023486, whole genome shotgun sequence.
ACCESSION   DAOQDO010000006 DAOQDO010000000
VERSION     DAOQDO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3672_length_9635_cov_3.023486"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKZ53_00280"
     CDS             <1..642
                     /locus_tag="PKZ53_00280"
                     /inference="COORDINATES: protein motif:HMM:NF038076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4015 family protein"
                     /protein_id="HNJ38896.1"
                     /translation="LRALFIPDMTFEECEISWILQTNITPTLQAYPNLEHLGVRGGSG
                     LSLGSIRHEHLKSLTIQTGGLNASVVRAVTAADFPELEHLELWLGTENYGATTEPVDL
                     APLFSGRLFPKLKYLGLRNSDFSDLIATAMADSPLLERLETLDLSLGTLGDEGAEALL
                     KSPATAKLRFLDLHHHYCSDEMMEKLKALGIKVDLSDAQGECDQDDRYVEVGE"
     gene            669..1811
                     /locus_tag="PKZ53_00285"
     CDS             669..1811
                     /locus_tag="PKZ53_00285"
                     /inference="COORDINATES: protein motif:HMM:NF038074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4014 family protein"
                     /protein_id="HNJ38897.1"
                     /translation="MVVIGNPNGQRIAHLQAALSRLNLSSAQVILYTDLIAGRVRLTD
                     VIEPGSVLRIESPDKDFETNRAFLILGAEQAASEQVDFLSPEQVDQLQFEKGRLWPSR
                     QWYLGFCTTLRLIEVQVAECPGVQYLQPPLAISRMFDKRLCQSLFAETGLPVPQILGP
                     PGCFEELLTTMYQTACHQVFVKLAHGSSAAGAVALRTSNGRFQATTTVEMVQEQGSLK
                     LYNSRKIRTYTNPKEIAELVDALCRHRVHVERWLPKAADGNGCFDLRMVVIDGQVHHI
                     VARTSRSPFTNLHLLNTRGDIEAIRARIPDAVWQCAVQSCERTISCFPGSFYAGIDLL
                     ITAGYRHHAVLEINAFGDLLPGVLHNGLDTYSAELRAWLNQYSGFG"
     gene            1872..2681
                     /locus_tag="PKZ53_00290"
     CDS             1872..2681
                     /locus_tag="PKZ53_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4013/SEN3800 family hydrolase"
                     /protein_id="HNJ38898.1"
                     /translation="MLDMHSLIGSHDVFFLTLDTLRYDVACQMLDSGHTPHLAALLPD
                     GRWEARHTPGSFTYAAHQSFFAGFLPTPIAPGPHPRLFATAFAGSETITQNTIVFNAS
                     DIVTGFASLGYHTICIGGVGFFNKLNPLGSVLPGLFAESYWSPSLGVTDPDSTRNQVR
                     LACELLERLPISQRVFLFINISALHQPNCHYLPGAQTDSAASQGAALAYVDRQLPPLF
                     SKLRQRGSVLGIVCSDHGTAYGEDGYSGHRLGHPVVWTVPYAEFVLKGFKL"
     gene            2678..3997
                     /locus_tag="PKZ53_00295"
     CDS             2678..3997
                     /locus_tag="PKZ53_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HNJ38899.1"
                     /translation="MKPVQSLWEQIPYEAYSYSYPHKTAYRPLDPPVFLKDLWESENR
                     QALFLYIHIPFCEMRCGFCNLFTTTNPDDEIPRAYLQTLTRQANQVRQALGPTTFARL
                     AIGGGTPTYLTVNGLETLFDLVETIFEINPHQIPVSVETSPLTAEPEKLRLLRERGVD
                     RISLGIQSFVESEVAAVGRRQKNTEVFQAIDRISQAGFPTRNLDLIYGLPGQTVESWL
                     TSLKTALTFSPEELYLYPLYVRPLTGLGRQAKQWNDLRLNCYREGRALLLDAGYRQLS
                     MRMFQSPTARAEKGPVYCCQEDGMIGLGCGARSYTQTLHYSNEYAVDAPAVRLILADY
                     LKQPDSAFELATYGIRLTEEDQRRRFVIQSLLQTSGLRLGAYRSRFGSTVFEDLPELR
                     SLIESDLAFHNDGFLQLSKYGLERSDLIGPWLYSPTVQTLMETFERR"
     gene            3994..4899
                     /locus_tag="PKZ53_00300"
     CDS             3994..4899
                     /locus_tag="PKZ53_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HNJ38900.1"
                     /translation="MKLSILYRGPLSSCNYGCQYCPFAKVQESTAEHRADAVALNRFV
                     TWVAGRMSDQLSVFFTPWGEALIHKRYQSAFIQLSHLPHIQKVAIQTNLSSPLHWIKN
                     CEVTRLGIWATFHPTEVLRARFVEKCWELHRQGVGLSVGVVGLKEHFDEIELLRQELP
                     ASVYLWVNAYKRMANYYTIQEIQQLSRIDPLFLINNHHHPSFGHTCRTGRSVISVDGN
                     GTIRRCHFVAEPLGNLYSADFEMVLKERLCPNETCGCHIGYVHLDNLQLYEVFGEGVL
                     ERIPVLRAETPSGSGLGAEDSQARG"
     gene            complement(5012..6190)
                     /gene="sucC"
                     /locus_tag="PKZ53_00305"
     CDS             complement(5012..6190)
                     /gene="sucC"
                     /locus_tag="PKZ53_00305"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNJ38901.1"
                     /translation="MKIHEYQAKALLAKYGVAVPRSHVAKTPQEALEAAEKLGSFPVV
                     IKAQIHAGGRGKGGGVKLARSMEDVETLASQILGMNLVTHQTGPEGRIVNTLLVEEGL
                     HIKKEFYLGLVIDRATSAPVFMASEAGGMDIEEVAASTPEKILKEYIDPTLGFQVFQA
                     RKLAFGLGLSDKLVGKAVKFMMALYRAFVESDASLVEINPFLLTEEGDLYALDAKMTF
                     DDNAMFRHKDYAELRDLNEEEPLEIEASKYDLNYIKLDGTIGCMVNGAGLAMATMDII
                     KLAGGEPANFLDVGGGASQQRVENAFRILLADEKVQAVLINIFGGIVRCDMVASGVIE
                     AARNLNVQVPIVVRLEGTNVERARELLDESGLNFTTASGMADAAEKVVGLAKSYAAAN
                     "
     gene            complement(6371..7624)
                     /locus_tag="PKZ53_00310"
     CDS             complement(6371..7624)
                     /locus_tag="PKZ53_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNJ38902.1"
                     /translation="MKLNIRNKLIIFAALLVFTPLALSAIAILLQVKGSIEDNAKKNI
                     EKDARLAEKIYKGRQQIIKEIAQNLAQAISAENLLESAQTPSTPASGGAAAAPSSAAV
                     TAQQSQLRGKRRIQELMAQRQDQVDFLIVTDKSGKVLLTNKGEPKGNESKLEDPLVLK
                     SKNNLDANQFADAALGGSVRVDAEALKSLELDSQLVKPGSSGLAIEGAAPILAGERSL
                     GLVIAGVLLDNAKQDRSIAAEIKEAMYSDLPSEAAAAVLNDTKTIISANFPSDSGKSA
                     VGQQIKRELLIDRPVAGTDNVHEIDYKSAFMPLKDPDGKNVGYVGVAIKESWFSAVIN
                     RVLLIIVAIFVVVLGLAIFAAFYFSQRLTQPIILLTEASNRISLGELDEAIAVGGSDE
                     IGQLAESLERMRISLKSAIERLRRR"
     gene            complement(7799..8608)
                     /locus_tag="PKZ53_00315"
     CDS             complement(7799..8608)
                     /locus_tag="PKZ53_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ38903.1"
                     /translation="MTDAFSGNLSQLKLIDVLRLLHASNRTGLLELTHDDGRTGEIYI
                     ESGQISHAMYEEYIGEEAIYNLFSWGAGMFRFRSGEVTDERTTSISTEEILLECVTYA
                     TEWESIRRVIPSPNTIFRLRSSWNQVREVSLRAEDWMVIQNMDGLRTVAEIAEITQLN
                     ELYTSKVIVRLFDLGLVEFVGEQEPEEEIIDSVPEELLLRIEKELTRAIGPMGPIVLD
                     DSAESLGFKRSQLPRDMMPALAERLAEEIPDTVRRVNFQEAMLEIMQKLYE"
     gene            complement(9130..>9635)
                     /locus_tag="PKZ53_00320"
     CDS             complement(9130..>9635)
                     /locus_tag="PKZ53_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNJ38904.1"
                     /translation="NKWSHMSKAHEVFLQEMMRIPGVKSAMLVNNRGEVLLSTGQFTL
                     NSQATIQMGTIITQSVSVAEGVYGKTDEISILYGEGRITTITNLDLLIESSYGVQEVF
                     VIIVSAPNANLPTIRLTVKVAASKLKLDKAIRDLKVSVRVDRRNLLSRDRLDATSWEL
                     FEAMNRA"
BASE COUNT         2283 a         2520 c         2394 g         2438 t
ORIGIN      
        1 ctgcgggcgc tgtttatccc ggatatgacc tttgaagaat gtgaaatctc ctggattcta
       61 caaaccaata ttacgccaac cttgcaagct tatccgaatc ttgaacatct cggcgtacgt
      121 ggaggctctg gtctttcgct gggatcaatt cggcatgagc atcttaaatc actcacgatt
      181 caaactggcg ggctcaatgc aagtgtcgtt cgagccgtta cagcggctga ttttccagaa
      241 cttgaacacc ttgaactctg gcttggaact gaaaactatg gcgccaccac cgaaccggta
      301 gatttggcgc cacttttttc tggcaggctc tttccaaaac tcaaatacct tgggctgcgc
      361 aacagcgatt tcagcgatct gattgcaacg gccatggctg attccccgct gttggaacgc
      421 cttgaaacgc ttgatctttc tttagggacg ctgggagatg aaggggcgga agcactgctc
      481 aagagcccgg caacggccaa attgcgcttc ctggacctgc atcatcatta ctgttcagac
      541 gaaatgatgg aaaaattgaa ggcgctgggc atcaaagtgg acctctctga tgcccaggga
      601 gaatgcgatc aggatgaccg gtacgtcgaa gtgggtgagt gatgagcagg ctcgttcctc
      661 ctttgcatgt ggttgtcatt ggtaacccga atggtcagcg gatagcccat ctgcaagcgg
      721 cactcagccg actcaatctc tcatcagcac aagtgattct ctatactgac ttgattgctg
      781 gacgcgttcg gctgactgat gtgatagagc caggctcagt acttcggatt gaatcacctg
      841 acaaagattt tgaaaccaac cgggcctttc tgatacttgg tgcggaacag gcagccagcg
      901 agcaggtgga ttttctttcg ccagaacaag ttgatcagct ccagtttgaa aaaggtcgct
      961 tatggccttc ccggcaatgg tatcttggat tttgtacaac gctccggctc attgaggttc
     1021 aggtagctga gtgtccaggg gttcagtacc tgcaacctcc tctagcaatt tccagaatgt
     1081 ttgataaacg cctttgtcag agcctgtttg ctgaaaccgg cctcccagtt cctcaaattc
     1141 taggcccacc tgggtgtttt gaagaactat tgacaacaat gtatcaaacc gcctgccatc
     1201 aagtcttcgt caagctggca catgggtcga gtgccgccgg agcggtggca ctgcgaacca
     1261 gcaatggtcg gtttcaagcc accaccaccg ttgaaatggt gcaggaacaa ggtagtctga
     1321 aactctataa ctcacgcaaa atccgaacgt ataccaatcc aaaggaaatt gcagagctgg
     1381 tagatgccct gtgccgtcat cgggttcacg tcgaacgatg gcttcccaaa gccgctgatg
     1441 gaaatggttg ctttgacctg cggatggtcg tcattgacgg tcaggttcac cacatcgtag
     1501 cccgaaccag tcgaagtcca ttcaccaatc tgcatctcct caacactcgt ggagatattg
     1561 aagccatccg ggcacgcatt ccagatgctg tctggcaatg tgcagttcaa agctgcgaac
     1621 ggaccatatc ctgtttccca ggaagttttt acgccggaat tgaccttttg atcactgccg
     1681 ggtatcgcca tcacgcggta ctggaaatta atgcttttgg tgatttattg cccggtgttt
     1741 tgcacaatgg gcttgatacc tattcagccg agctacgtgc ctggctcaat cagtattcag
     1801 ggtttgggta aaagagaaat cggaccaaag gacatttctt gaccctaaac gttgaagccc
     1861 aaaccctcac tatgcttgat atgcattcgt tgattggctc acatgacgtg ttctttctca
     1921 cacttgacac gttgcggtat gacgtggctt gccagatgct ggactcaggt cacacaccac
     1981 acctggctgc cctgttaccg gatggacgat gggaagcgcg ccacacgcca gggagtttca
     2041 cctatgcggc ccatcaatcg ttttttgctg gttttttgcc aacgccgatt gcacctggcc
     2101 cgcacccacg attgtttgct acggcttttg ccggaagcga aaccattacc cagaacacca
     2161 ttgttttcaa cgcctcagac attgtgactg gatttgccag ccttggctat cacaccattt
     2221 gcattggagg agttggtttt tttaacaagc tcaatcccct gggaagtgtg ttgcctggac
     2281 tttttgctga aagttactgg tcgccatctc tgggcgtgac ggatccagat tcaacccgca
     2341 atcaggtacg gctggcctgt gaacttctgg aacgtctccc aatcagccag cgagtctttt
     2401 tgtttataaa tatttccgcc ctccaccagc caaactgtca ctatttacca ggcgcacaaa
     2461 ccgattcggc ggcgtcacaa ggcgccgcgc tggcctatgt tgatcgtcaa ttgccaccat
     2521 tgttttcaaa gctcagacaa cgtggttcag tactgggaat tgtctgctct gaccacggta
     2581 cggcgtatgg agaggatggc tactctgggc atcggcttgg tcacccagtg gtctggaccg
     2641 tcccctacgc tgagtttgtt ttaaaaggat tcaagttatg aaaccagtgc aatccctttg
     2701 ggaacaaata ccttatgagg cgtactccta ttcatatccg cataagacag cgtatcgtcc
     2761 gcttgatcca ccagtttttc tgaaagacct gtgggagagt gagaatcggc aggccctctt
     2821 tttgtatatt cacattccgt tctgcgaaat gcgatgcggg ttttgcaatt tgtttaccac
     2881 caccaatccg gatgacgaaa ttcccagagc gtacctgcaa acgctcacgc ggcaggccaa
     2941 tcaggtccgc caggcccttg gcccgacaac ctttgcccgc ctggcaattg gtggagggac
     3001 tccgacttac ctgaccgtga atggactgga aacgttgttt gatttggtgg aaaccatttt
     3061 tgaaataaat ccccatcaga ttccagtttc agttgaaacc tcgccactca cagcagaacc
     3121 tgagaagttg cgcctcttac gagagcgggg tgtagatcgc atcagtcttg gaattcaaag
     3181 ctttgtcgaa tcagaagttg cggcggtggg ccgtcgtcag aaaaatactg aggtgtttca
     3241 ggccattgac cgaatcagcc aggctggttt tccaactcga aacctcgatt tgatctatgg
     3301 attaccggga caaacagtcg aaagctggct cacttcactc aaaacggcat tgacgttttc
     3361 accagaggaa ctctatttgt acccgctcta tgtccggcca ttgacggggt tgggacgcca
     3421 ggcaaagcaa tggaatgatc tccgactcaa ctgttatcgc gagggccgcg cccttctgct
     3481 tgatgccggg tacagacaac tttccatgcg gatgtttcag tccccgactg cccgtgctga
     3541 aaaaggaccg gtgtattgtt gtcaggaaga tgggatgatt ggtctgggtt gtggtgcacg
     3601 gtcctatacc cagacactcc actactcaaa tgaatatgcg gttgatgcac cagccgtccg
     3661 attgattcta gcggactatt taaagcaacc agactccgcg tttgaactgg caacttatgg
     3721 aattcggctc acagaagaag atcagcgccg tcggtttgtg attcaatcgt tgctgcaaac
     3781 cagtggcttg agacttgggg catatcggag ccgatttggg tctactgtct ttgaggatct
     3841 tccagaacta agatcactga ttgaatcaga cctggctttc cacaatgatg gctttctcca
     3901 actttccaaa tatggtctgg agcgatctga cctgattggc ccctggttgt attcaccaac
     3961 tgtccaaaca ctgatggaga cgttcgaacg ccgatgaaac tgtcaatcct gtatcgtggg
     4021 ccgctctcaa gctgcaacta tgggtgccag tattgcccat ttgccaaggt tcaggaatcc
     4081 acggctgaac accgggccga tgcagtggcg ctgaaccgat ttgttacctg ggttgctggc
     4141 cggatgtctg accaactctc agttttcttc acgccctggg gagaagcact catccataaa
     4201 cgctaccagt cagcgttcat tcagctcagc catctaccac atatccagaa agtcgcgatt
     4261 cagaccaatc tttccagccc gctccactgg atcaaaaact gtgaggtaac tcgactcggt
     4321 atttgggcca cgtttcaccc gacggaagtt ttgcgagcgc gatttgtgga aaaatgctgg
     4381 gaattgcacc ggcaaggggt tggattgagc gttggtgtcg tgggtctcaa ggaacatttc
     4441 gacgagattg aattgctccg acaggaatta cccgcctcag tctatctatg ggtcaacgcg
     4501 tacaagcgga tggccaacta ctacaccatt caggaaatac agcaactctc cagaattgat
     4561 ccgctgtttc tcataaacaa tcaccatcat ccaagttttg ggcacacctg tcgaaccggg
     4621 cgttctgtca tttccgttga tggaaacgga accattcgcc gctgtcattt tgtagctgag
     4681 ccacttggga atctgtattc ggctgatttt gaaatggtgc tgaaagaacg tctgtgtcca
     4741 aatgaaacct gtgggtgcca tatcggctat gttcatctgg acaacctaca actctatgag
     4801 gtttttgggg agggagtgct ggaacgaatt cctgttttga gggctgaaac cccctcgggc
     4861 tcagggcttg gggctgaaga ctcgcaagct cggggctgaa gacgtttggg ctgaagacat
     4921 cgggctgaaa aactggttgt atttcatccc tcatccttca tccctcatcc cttcaattgc
     4981 cccaagccca atgtcttcag cccgaagtct ttcagtttgc tgcggcgtaa ctcttggcca
     5041 ggccgacgac cttttcagcc gcatcggcca tcccagaggc cgtggtgaaa ttcaaccctg
     5101 actcgtcaag cagctcacga gcacgttcga cgttggtgcc ttcgaggcgc accacaatcg
     5161 gcacctgcac gttcaggttg cgggcggctt cgatcacacc actggccacc atatcgcacc
     5221 ggacaatccc gccgaagatg ttaatgagca cggcctgcac tttttcatcg gccagcaaaa
     5281 tccggaaggc attttcaacc cgctgttgcg acgcaccacc accaacgtcc agaaagttgg
     5341 ctggttcgcc accagccagt ttgatgatgt ccatcgtggc catcgccagc ccggcgccgt
     5401 tgaccataca gccaatggtt ccatccagct tgatatagtt caaatcgtac tttgacgctt
     5461 cgatttcaag tggttcttct tcgttgaggt cgcggagttc ggcatagtct ttgtgccgga
     5521 acatggcgtt gtcatcaaag gtcatcttgg catccagcgc atagagatcc ccttcttcag
     5581 tcaacaggaa cgggttgatt tcaaccagcg acgcatcgct ttccacaaac gcccgataga
     5641 gtgccatcat aaatttgacg gctttgccga ccagcttatc ggaaagtcca aggccgaagg
     5701 ccagtttgcg agcctggaag acctgaaaac caagggttgg atcaatgtat tctttcagga
     5761 ttttctcggg tgtcgaagcg gcaacttctt cgatatccat tccaccagct tcgctcgcca
     5821 taaaaactgg cgcggaggtg gcccggtcaa tcaccagccc cagataaaat tcctttttga
     5881 tgtgcaagcc ttcttcgacc agcagtgtgt tgacgattcg gccttccgga ccagtttggt
     5941 gcgtaaccag attcatgccc agtatttgcg aagccagggt ttcaacatct tccattgacc
     6001 gggccagttt gacgccaccg cctttcccac gaccgcccgc atggatctgg gccttgatca
     6061 cgaccgggaa agaaccaagt ttttcagccg cttcgagagc ttcctgagga gtttttgcga
     6121 cgtgactgcg tggaacagca actccgtact ttgcaaggag ggctttcgcc tgatactcgt
     6181 ggattttcat gaatcacctt ctcaactgat gtgaataaaa gaaaaagaat gcaatgaggg
     6241 ttcagggttc agggcttcaa agttttcaaa acccatcctg gtgaaaaaac aaatgacaag
     6301 aggcaggcaa tgcgttggta catcacctgt cgcctgtcat tcaagagcaa tttacggctg
     6361 gcacaatcac ttaccggcgg cgcaaccgtt cgatggcgga tttcaagctg atgcgcatgc
     6421 gctccagcga ttcagccaac tgaccaattt cgtccgaacc acccaccgca atcgcttcgt
     6481 caagttcccc aaggctgatt cggtttgagg cttcggtgag caaaataatg ggttgggtca
     6541 ggcgttgtga gaaatagaac gccgcaaaga tagccagtcc gagcacaacc acaaaaatgg
     6601 ccacaatgat caagagcacg cgattaataa cggcactaaa ccagctttcc ttaatcgcaa
     6661 ccccgacata cccgacgttt ttgccatctg gatccttaag tggcataaac gctgatttat
     6721 agtcaatctc gtggacgtta tcggttccag ccactgggcg atcaatcaaa agctcacgct
     6781 taatctgttg tccaaccgcc gacttgccgc tgtcggaagg aaaattcgct gaaataatcg
     6841 tcttggtatc attcagaacg gcggcggcag cttcacttgg cagatctgaa tacatggctt
     6901 ctttgatttc cgccgcaatt gagcgatcct gtttggcgtt gtcgagcaac accccggcaa
     6961 tcaccaatcc aagtgaccgt tcacccgcca aaatgggagc ggccccttca atcgccaatc
     7021 cggaagaccc tggcttgaca agctgtgaat ccagttcaag cgattttaac gcttcagcat
     7081 caacccgcac cgaaccgccc agggcagcat cagcaaactg gttcgcgtcg aggttgtttt
     7141 ttgacttcaa caccagcgga tcttccagtt tggattcatt gccctttggt tcgcccttat
     7201 tggttaaaag taccttgccg ctcttgtcgg tcacgatcag gaaatcaacc tgatcctggc
     7261 gctgagccat caattcctga atccgtcttt tcccacgcaa ctgactctgt tgggcagtca
     7321 ccgcggcact tgatggagcc gccgccgcac cgccagatgc cggggttgaa ggggtttgag
     7381 ccgattcaag caaattctcg gctgaaattg cctgtgccag attctgggca atttccttga
     7441 tgatctgctg tcggccttta taaatctttt cagccaggcg ggcgtctttt tcgatattct
     7501 ttttggcgtt atcctcaatg ctccctttga cttgcaaaag gatagcaatg gccgaaagag
     7561 ccagcggggt aaacacgagc agggcggcaa agataatcag tttattgcga atgttgagtt
     7621 tcatcagggt cgtcgcgctc agggtgcatg atggttcagc agatccagga agcgcctcct
     7681 tcgttcaaaa tgttctaaac gcagcggcct gggtcacagg caggcaatgt gagtgatgct
     7741 tggtttgtgg tgcctggtac gttcatcgtc aaaagtactg ggtcccaagc accagggctc
     7801 attcatacag cttctgcatg atttcaagca tggcttcttg gaaattgacc cgtcgaaccg
     7861 tgtcagggat ttcttccgcc agacgttcag ccagagcagg catcatatcg cgcgggagtt
     7921 gagaccgttt aaatccaagc gattcggcac tgtcatcaag cacaatcggc cccatggggc
     7981 caatggcgcg ggtcaattct ttttcaattc gcagcaacag ttcttccggg acagagtcaa
     8041 taatttcttc ttccggttcc tgctcaccaa caaattcaac cagccccaaa tcaaacaggc
     8101 gaacaatcac ctttgaggta tacaactcgt tcaactgtgt aatttcagca atttcggcaa
     8161 cggtgcgcag tccatccata ttttggatga ccatccaatc ttcagctcgc agggaaactt
     8221 cccgaacctg attccaggac gaacgaagac gaaagatggt attgggcgat ggaatcaccc
     8281 gtcggatgct ttcccattcc gtggcatatg tcacgcattc cagcaaaatt tcttcggttg
     8341 aaatcgaagt ggttcgctca tcggtgactt ctccggaccg gaatcgaaac atccccgccc
     8401 cccacgaaaa gaggttatag atggcttctt cgccgatata ctcttcatac atcgcgtgag
     8461 aaatctggcc actttcgatg taaatttcac cggtccgacc atcatcatgg gttaactcaa
     8521 gcagcccggt tcggttgctt gcgtgtaata accgcaacac gtcaatgagt ttaagttgag
     8581 atagatttcc actgaaggcg tctgtcatat gtgttggctt ggatggctaa agtcaaagtc
     8641 actgttttga gttacgggtc gctgtgggtt tggccagttt gtgtgggccg tagacgcaga
     8701 gaaagcgacg tcaagctagg ccgcgctcca aaatatcgta cccgtgtggg ctgtggacgc
     8761 aggatgtacc gccttggctt tcgaaaatga agttttggtg gacgggcgct gacgatcagc
     8821 ctgaagcagg aggatcgccg gagttctgga acgaagactg aggctgacaa ttcgggagaa
     8881 aaccatcgga ctggcctctc tgcaccaggg tacggaggcg actcctgcgg cgtgatcaaa
     8941 ctgctttcct ttcaaggaac caatccatct tttcaacgaa agatattgac atcattcgtg
     9001 tatctgaaat ttcagcgacg gctgatttgg gcttcaatcc ctggaaaaga accacctcac
     9061 tggaagcaac cagccgattg ccgggtctat gaattgggaa cgaggtgctc accagtgcca
     9121 tttaccgggt taagcccggt tcatggcttc aaataattcc caactggtgg catcaaggcg
     9181 atcacgagaa agcagattgc ggcggtcaac tcggacactg acttttagat cccgaatggc
     9241 tttgtcgagt ttgagtttgc tggcggcgac ctttacggtc aggcggattg tcggaagatt
     9301 ggcatttggt gcactgacga tgatcacaaa gacctcttgc acaccatacg aactttcaat
     9361 cagcaagtca agatttgtga tggtcgtgat tcgaccttct ccatacagga ttgaaatttc
     9421 gtcagttttc ccgtaaaccc cctcagccac cgataccgat tgggtaataa tggtgcccat
     9481 ctggattgtg gcctgtgaat tgagggtaaa ctgcccggtg ctgagcaaga cctcgccccg
     9541 gttattgacc agcatggcgc ttttgacgcc tggaatccgc atcatttcct gtaaaaacac
     9601 ttcatgagct tttgacatat gactccactt attgt
//
LOCUS       DAOQDO010000007         9395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3834_length_9395_cov_3.760921, whole genome shotgun sequence.
ACCESSION   DAOQDO010000007 DAOQDO010000000
VERSION     DAOQDO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_9395_cov_3.760921"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..899)
                     /gene="xth"
                     /locus_tag="PKZ53_00325"
     CDS             complement(108..899)
                     /gene="xth"
                     /locus_tag="PKZ53_00325"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNJ38905.1"
                     /translation="MTKPFTVATWNVNSIRSRLTHVIDWLRAHSPDVLCLQETKVQDT
                     EFPVEPFEEAGYIATFYGQKSYNGVAMLSLDEPEAVQRGFPNEPADAQRRLLAATFDG
                     IRLVNVYIPNGGESPKDPKFTYKMQFLEQLETYLTTMYTPQTPLLVCGDFNIAPGDLD
                     LFEPDKYRESVMFHSREHAFLNRWETWGLVDLVRQQHPDQPELYTWWDYRTTAFPRNR
                     GWRIDHIWVTPPLVERCALTRIDRDERGKDKPSDHVPVVAGFHRE"
     gene            1137..1289
                     /locus_tag="PKZ53_00330"
     CDS             1137..1289
                     /locus_tag="PKZ53_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38906.1"
                     /translation="MDYRLEAISGMGVYADGTTQILLWLWGFDSKTTPSTWIVEASPF
                     SQTEVS"
     gene            1809..2636
                     /locus_tag="PKZ53_00335"
     CDS             1809..2636
                     /locus_tag="PKZ53_00335"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNJ38907.1"
                     /translation="MVSYFGNLLIKPWEHLEEPYFNEVSMAWPCFIQVAYMQQKNLFE
                     VEEATSLHVQPPDSPESETCTSHWEKIFWAQGYTRLAGVDEVGRGAWAGPVITAAVIL
                     DPENIPTGLNDSKKLSAPQRTYLAELITQSALSLAFGTQSATEIDTFNILEATRRAMM
                     QAIEQLAPTPDFLLIDAVHLPKVKIPRQSIIRGDAQSVSIAAASIVAKVYRDQLMTAF
                     DTQYPGYGFARHKGYGTAEHQAALEKLGPCVIHRLSFRGVVKKEADFGLLSPVEPSL"
     gene            2809..6174
                     /locus_tag="PKZ53_00340"
     CDS             2809..6174
                     /locus_tag="PKZ53_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ38908.1"
                     /translation="MSFSKANDLKRAEKLVQQGKIAAAIKEYSNLVAANPSDIMLLNT
                     LGDLCVRDGRIDEAILYFTKIGENYSKNGFTLKAIAMYKKIYKLAPNNPEIAYKLAEL
                     YAKQGLIVDARKQYMEVADAYTRAGRTQQALDVLRKIADLDPENVSVRLKLAERYQSE
                     RMNDKAHEAYIAAGSQLQRKGNLEESQQCYLKALGVDPSSKPALSSLANIFIQLGDPQ
                     RAISMLNRVIDNNPNDPDLVTLMGRTYLSAEMLDDAEKAFLRLHDIDKTRFEYLLQLG
                     RKFADQGQFNRAVDTVNACLDTVITRRQESKAIDLLDAIIELDPYHIPTYRCLKAIYT
                     RMGEDNNLSNALTGLVEAALHHQDRDTAGEALRQLVELEPNDSSHRRRLEALGLSTAI
                     KPTLVDRDTGPSSSLTPKLGPGIGSTADFKFGTDLYPDSNQGFTGLTSGTGFTSGTGF
                     SADSGFSPAELPSQGFGGFGAPPPPTFPVQEGPTGFSGFVSGSQFSTPDRGAGSPNLF
                     ATVPPVSTGPDIQKLLMDAERYASRGYADRAAEILREVISIDPHHFEARLKLRDIYLS
                     GGLTTEAAEQCLALAHIYDAAGNSAQAQKMLTEAFSLNPNATPGIPQPPATHQAPEDA
                     IEIDISGGFDGGISGSFNSYAPPSPPPPPPPVFEDHFYSGAPHHVAPPPGDLSFAPLT
                     DTSHVQDTLREELEGVDFYLSQGFTDLAADTLNRLNAQFPNHPEIQSRMAKLAPAPPL
                     GGLPPAPPVSSFDFSGAMSAGPSAVAPPPPAEYDFGGFGAPPPIAAGPVSSPPPFSNF
                     SGSGGFMTGGSYPGEIPPPAPPISAFQPPVAAPPPMEFQPPVAAPPPISTFQPPVAAP
                     PPISTFQPPVAAPPPMEFQPPPPGPNLFGAPAVTPPPPAYDMTSAMMAGPPSGDLLIE
                     SLITDIGDSLDGIDDLEMVNRITGAHHQASVPPPPPPSIAAPAPVAQGMFRGLEESGL
                     QDIFDEFKHSIEQEEAEEAPDFETHYNLGLAYKDMELFDEAVEEFQAAIKATDPHAPD
                     GHYFQSCNMLGLCFMSKAMYPPAAVWFKRGLDAPGRSEDEYQAMRFDLGLAHEMQGKL
                     ESALELFEIVYAIDINYRDVGEKIKEIKEKLRK"
     gene            6363..6542
                     /locus_tag="PKZ53_00345"
     CDS             6363..6542
                     /locus_tag="PKZ53_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38909.1"
                     /translation="MFYLTSYFHSDVFLQYMQSGYVRGAVTGLGIINVVMGFLEILHF
                     RQTVDTILQSAPAKK"
     gene            6730..7365
                     /locus_tag="PKZ53_00350"
     CDS             6730..7365
                     /locus_tag="PKZ53_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNJ38910.1"
                     /translation="MQVYLISNRRILFAPTETFSEDQARKRLIALGEQAARMSVDFFQ
                     LREKDLPSRICFEVARELATLFHHTDTQLLINDRVDIALASGANGVHLTTTSLLATQI
                     RTWAPAELTIGISTHTAAELHRTSPGASFAVCGPVFETPGKPRLGLEPLTQIIHTSPV
                     PVFALGGITVDNAAELKQTGAIGVAGIRLFAQAALRGPTEFCDLISYLKAL"
     gene            7362..8174
                     /gene="thiD"
                     /locus_tag="PKZ53_00355"
     CDS             7362..8174
                     /gene="thiD"
                     /locus_tag="PKZ53_00355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNJ38911.1"
                     /translation="MKTILSIAGLDPAGGAGLLADLKTIAAHGHHGVGVLTAATAQNT
                     VGVKSFYPLTPEAVVAQLDALHEDLVLAGVKTGMLGTGAIAEAIADWFHRHWTSPVVV
                     DPVLVASSGHLLFDLEAIDVIRKRLLPLATVITPNLPEAATLTGRTVETLSQMEDAAR
                     ELYTYGPRYVLMKGGHLMTEAMDVLFDGEQFHYFPGTRLEVYTHGTGCTLSAALASLL
                     GSGFTIVEAVKRAKAYVRVAMQHTLCVGKGKELLGHLPLDLLREHNTTITSD"
     gene            8240..>9395
                     /gene="lptC"
                     /locus_tag="PKZ53_00360"
     CDS             8240..>9395
                     /gene="lptC"
                     /locus_tag="PKZ53_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNJ38912.1"
                     /translation="MSFSFPKLFKYLSLAAFLGVVVYLAIQLSSRNNPLRPKKPTDLS
                     VQLVGVSNDFRYVHRDQNVDRLILTAAKDTAFTNGQHQLEEVKLETFGQDGKPLGVLT
                     SKTADYDSQAQVAVFKKDVLVVTTDELVVRTNEMKYDQAANVTSTEAPVTFTRRNITG
                     TCVGAKLDSTAGRFVMNQQVKITIAPESTAPASPVPSTTASAASPPQSAPDQDKKDKK
                     DKKNKKDKKDKKDKKANRVKETGNQPSTASKPASTSPALTAEIPTTITGDHAEYWKPE
                     KQVYLTGNAIILQGGNELRADKMTAFLDDNQRIQKLETRARSFLKSAEKQGQVESQDM
                     DITFTSDGDVQSALATGNPVVKSFEGPAPREVTGDRIEVSFAKVNEVNALQ"
BASE COUNT         2421 a         2462 c         2218 g         2294 t
ORIGIN      
        1 caactttttc agcccgagat gtcttcaacc caacttcttc agcccgactt cttcagccca
       61 acttcttcag cccgacttct tcagccccat cttctccaac tccacgccta ttcccgatga
      121 aacccagcca ccactggcac atggtcagaa ggtttatctt tccctcgctc gtcacgatca
      181 atccgtgtta aagcacaccg ctccaccagt ggtggcgtga cccagatatg gtcaatgcgc
      241 cagccacgat tgcgtggaaa agccgtcgtt cgataatccc accaggtata gagttctggc
      301 tgatcagggt gctgttgtcg caccagatca accaatcccc aggtttccca tcggtttaaa
      361 aacgcatgtt cccgcgaatg aaacatcacc gattcgcggt acttgtctgg ttcaaaaagg
      421 tctaaatctc ccggagcaat attgaaatca ccacatacca ggagtggggt ttgtggagta
      481 tacatcgtgg taagatatgt ctctaactgc tctaaaaact gcattttata agtgaatttc
      541 ggatcttttg gtgattcgcc accattggga atgtacacgt tgaccagtcg gatgccatca
      601 aatgtggctg ccaggagtcg tcgttgagca tcggctggct cgttggggaa cccccgttgc
      661 accgcttcag gttcatccag ggaaagcatc gccacaccgt tataggattt ctgcccgtaa
      721 aacgtcgcga tatagcctgc ttcttcaaac ggttcaacgg gaaattcagt atcctgaact
      781 ttggtttcct gcagacacaa cacatctggc gaatgggccc gtaaccagtc aatcacgtgt
      841 gttaaacgcg aacgaattga atttacattc caggtagcaa cggtaaacgg ctttgtcatt
      901 ttcttttctc cagtgacagg gtcgtccaga atggacagga tttgaattga gttctgcttg
      961 tttgagagca ggttcaagtt caaaaactaa aagaacagaa tgaactgttt tcttttgaag
     1021 gagttacttt aaacgttctg tgaaaacttg aaactggcat atggcttgca caggcagttt
     1081 aggtcgaggt tttctgcgaa aacctatggt caatctcatc ttaaggcaac cggctcatgg
     1141 attaccgcct tgaagccata tcaggaatgg gtgtctatgc ggacggtaca acccagattt
     1201 tgctttggct gtggggcttt gattcaaaaa caaccccgtc aacctggatt gtggaggcat
     1261 cacctttttc acagacagag gtgtcataat tgtggggtat ctctttcatt ttccttctgg
     1321 tatccttggt tggttggagg ctgggtgggg tttctggctg gactcgggat tgtgctctgg
     1381 caaaactcaa ccccccaacc ggtacagttt ccacaattga caaccggatc aagcaaccag
     1441 accttgcccc aggcgaatac caactctacc gctcaagccc cccgcgacag catccattct
     1501 tctcaacccg cagatgagtc tcatgtttgt ggagcgaaaa ccaaaagcgg gaggccctgc
     1561 cgtcgtaaag tcagaggcac ccaaggctat tgctggcagc atcggaagtc aaccgaccag
     1621 agatgagcgc gtggggattg gttcctcgtt cttggaaaat atggggaaac cgaagcctct
     1681 caagatctcc tctaaacacg tattttggaa tcgctcccgg cttgtctttc agatcaatgt
     1741 ttccctggtt tgtcgcctat tgaaatctca gacatcattg aagaatttgg gagttctctc
     1801 tggtttttgt ggtttcttat tttggaaatt tattgatcaa accctgggaa catcttgaag
     1861 aaccctattt taatgaagtc tcaatggcct ggccatgttt cattcaggtg gcatatatgc
     1921 agcagaaaaa cttgtttgag gttgaagaag ccacttctct gcacgtacaa cctcctgact
     1981 ccccagagtc ggaaacctgt accagccact gggaaaagat tttttgggca caagggtaca
     2041 cacgtctggc tggagtggat gaagttggtc gtggtgcctg ggcaggtccg gtgattacag
     2101 cggcagtcat cctggaccca gaaaacattc cgaccggact caatgactcg aaaaaactat
     2161 cggcacctca gcgcacgtat ctggcagagt taattacaca atcggcactc agcctggcat
     2221 ttggaactca atcagcaact gaaattgaca cgttcaatat ccttgaagca acacgtcgag
     2281 ccatgatgca ggccattgaa caacttgctc ccacgccaga ctttcttctg attgacgccg
     2341 tacatcttcc caaggtgaag atcccaaggc agtcaatcat tcgcggagat gctcaatcag
     2401 tgtcaattgc cgccgcttca attgtggcaa aagtatatcg cgatcagttg atgacggctt
     2461 ttgacacaca atatccgggg tatggctttg cacggcataa aggctatggg actgccgagc
     2521 atcaagccgc acttgagaaa ctcggcccct gtgtaattca tcgtttaagt ttccgtgggg
     2581 tggtcaaaaa agaggccgat tttggattat tatcaccggt tgaaccatcc ctttgaaatc
     2641 caactcaatg cgattggatt tcccctaact gctttataga gtgtaatttc ccaggggttt
     2701 acacactggc aatgcggttg acaacgccct accagtgtgc tagcttgcca ttccggtctt
     2761 cgacctccac ctcgaccatc tctgtcccac tgctgcggga gaagcgccat gtcctttagc
     2821 aaagctaacg atttaaaaag agccgaaaaa ctcgttcagc aagggaaaat tgcggctgca
     2881 attaaagaat acagtaacct ggttgcagct aaccccagcg acattatgtt gctcaatacg
     2941 ctgggcgatc tgtgtgttcg cgacgggcgg attgatgagg ccattctcta ctttaccaag
     3001 attggtgaaa actacagcaa aaatggcttt accctcaaag ccatcgccat gtacaagaag
     3061 atttacaagc tggcgccaaa taaccctgaa attgcttata aattggctga actgtatgcc
     3121 aaacaagggt taattgttga tgcccgcaaa cagtatatgg aagtggctga tgcctatacg
     3181 cgggccggtc gcacacaaca ggcgcttgat gtcctgcgca aaatcgctga tttggatccc
     3241 gaaaacgtgt cagtgcgtct caaactggct gagcgatacc agagcgaacg catgaatgac
     3301 aaggctcacg aagcctacat tgcagccggg tcgcaactgc agcgtaaagg aaatctggaa
     3361 gaaagccagc agtgttatct gaaagcgctg ggagttgatc catcaagcaa accagcactg
     3421 agttccctgg ccaatatctt catccagttg ggagacccgc aacgtgctat ctcaatgttg
     3481 aatcgggtca ttgacaacaa cccgaatgat ccggatctgg ttaccttgat gggccgcacc
     3541 tatttgagtg cggaaatgct cgatgatgct gaaaaagctt ttctccggct tcacgacatt
     3601 gataagactc gatttgaata cctgctccag cttggtcgaa aatttgctga tcaaggacaa
     3661 ttcaaccggg ccgtagatac ggtcaatgcc tgccttgata ccgttattac ccggcggcag
     3721 gaatcaaaag cgattgattt gctggatgcc attattgaac tcgaccccta tcacatccca
     3781 acctatcggt gtctcaaagc gatttacacc cggatgggtg aggacaataa cctcagtaat
     3841 gcactcaccg gattggttga ggccgcactc catcatcaag atcgagacac agcaggcgaa
     3901 gcactccgcc aactggttga gcttgagcca aatgattctt cccatcgccg acgactggaa
     3961 gcgcttgggc tgagcacagc catcaaacca acgctggttg atcgtgacac aggcccatcc
     4021 agttcgctta cccccaaact tggtcctgga atcggaagca cagccgattt caaatttggt
     4081 actgacttat accccgacag caatcaggga ttcacggggc tgacctctgg caccgggttt
     4141 acctctggta ccgggttttc agcagattca ggtttttcac cagccgaatt gccatcccag
     4201 ggatttggcg gatttggggc cccaccacca ccgacgttcc cagtccagga agggccaacg
     4261 gggttcagtg ggtttgtttc tggaagtcaa ttctcaacgc ctgatcgggg tgcagggagt
     4321 cccaatttat ttgcgacggt tccacccgtt tcgactggac cagatatcca aaagttattg
     4381 atggatgctg agcgatatgc cagtcgaggc tatgcagacc gcgccgccga aattctgcgc
     4441 gaagtcatct cgatagatcc acatcacttt gaagcacggc tgaagctgag ggatatttat
     4501 ctgagtggcg ggttaaccac tgaagcagca gaacagtgcc tggccctggc tcatatttat
     4561 gatgcggcag gaaattcagc tcaggctcaa aaaatgctca cggaagcctt ttcgctgaat
     4621 ccaaatgcga ccccaggaat acctcagcca ccagcaacac atcaagcacc agaagatgct
     4681 attgaaattg atatcagtgg cggctttgat ggaggcatca gcggcagctt taattcgtat
     4741 gcgccaccat ccccgccgcc accgccgcca ccagtttttg aagatcattt ttattctgga
     4801 gcaccacacc acgtggcgcc accacctgga gacctcagct ttgctccatt gactgacacc
     4861 tcacacgttc aggatacatt gcgtgaagaa ctggaagggg tggactttta tctcagtcag
     4921 gggtttaccg acttagctgc cgatacactc aaccgactca acgctcaatt cccaaatcat
     4981 cctgaaattc agtctcggat ggccaaactg gccccagcac ctccgcttgg gggattgccc
     5041 ccagctccgc cagtatcgtc gtttgatttt tcaggcgcca tgtcagccgg accatcagcc
     5101 gtggctccac ctccgcctgc tgagtatgat tttggcggat ttggcgcccc acctcccatt
     5161 gcagccggac cagtttcaag cccaccgcca ttttccaatt tctcagggtc aggtggattt
     5221 atgactggcg gctcatatcc aggcgagatc ccaccaccag ccccaccaat cagtgccttt
     5281 cagcctccgg ttgcggcccc accaccaatg gagtttcaac ctccagttgc ggcaccgcca
     5341 ccaatcagta cttttcaacc tccagttgcg gcaccgccac caatcagtac ctttcaacct
     5401 ccagttgcgg caccgccacc aatggagttt caacctcctc cgcccggacc aaatttattt
     5461 ggggcaccgg ctgtcacgcc gccgccgcca gcttatgata tgacctcggc gatgatggcg
     5521 ggtccacctt cgggtgattt gttgattgaa agcctgatta ccgacattgg tgacagcctg
     5581 gatggcattg acgatttgga aatggtcaac cggattaccg gggcccatca tcaggcttca
     5641 gttccgccgc cgccgccgcc atctatcgcg gcacccgccc ctgtggccca gggaatgttt
     5701 cgagggctcg aagaatctgg ccttcaagat attttcgacg aatttaaaca tagtattgaa
     5761 caggaagaag ctgaagaagc acctgacttt gaaacccatt acaatttggg gttggcctat
     5821 aaagatatgg aactctttga cgaagcagtc gaagaatttc aggcggctat taaggcaacc
     5881 gacccacacg ctcctgacgg gcattatttc caatcctgca atatgcttgg gctgtgcttt
     5941 atgagcaaag ccatgtatcc gccggcggcg gtttggttta aacgtgggtt agatgctcct
     6001 ggccgaagtg aagacgagta ccaggccatg cgatttgacc ttgggttagc ccacgaaatg
     6061 caaggcaaac tggaaagcgc gctggagtta tttgaaatcg tgtatgccat cgacatcaac
     6121 tatcgtgatg tcggagaaaa aattaaggaa attaaagaaa agcttcgaaa atagcgggga
     6181 cgaagctttc cacaccaggt cttgtcagac ctggctttca cgtgtgaatt ccctgccatt
     6241 gcgcccatca attacctgtg tctgctaagc tcactgttgt tttttatatt cttatctgct
     6301 ttgaaatcgg ggcgttattg atcattcttc cctggtcttc atattggaat gagaactttt
     6361 ttttgtttta tctgaccagc tacttccact ctgatgtgtt cctccaatat atgcaaagtg
     6421 gatatgtccg tggggccgtt actgggctgg ggattatcaa tgttgtaatg gggtttcttg
     6481 aaatccttca ctttcgccaa acagttgata ccattctgca atcggcgcca gccaaaaaat
     6541 aaaaaaccgg ttcaagacct ccctcaccct caaatagaaa tctccctgcc ctccccccaa
     6601 tccagggtgc gagagaaaac tgattttttg tgcttcccct cactcacaac atcacaacct
     6661 cccctgatac caggacgtgc caggtcctga ttttagaaca tagcaccggt gtcctaaacg
     6721 taaatcacga tgcaagtcta tctcatttct aaccgccgga tcttatttgc accaaccgaa
     6781 actttttctg aagatcaagc cagaaaacga cttattgccc tcggagaaca agcggcaagg
     6841 atgagcgtag atttttttca gttacgagaa aaagaccttc cctctcgaat ctgctttgaa
     6901 gtagccaggg aactggccac actttttcat catactgata cccaattgct gatcaatgat
     6961 cgggttgata ttgctcttgc atcgggtgcc aacggcgttc atttgacaac aacctcgctc
     7021 ctggcaactc agattcgtac ctgggctccg gcagagttga caattggcat ttcaacacac
     7081 acggcagccg aacttcaccg cacgtcacct ggtgccagct ttgctgtgtg cgggccagtc
     7141 tttgaaactc caggaaaacc tcggcttgga ctagagccat tgacgcaaat cattcatact
     7201 tcaccagtgc cagtatttgc gttgggaggg ataaccgtag acaatgcggc tgagctcaaa
     7261 caaactggag cgataggtgt ggcgggaatc cggctttttg cccaggccgc actccgaggg
     7321 cccactgaat tttgcgatct tatttcttat ttgaaggcgc tatgaaaact atcttatcaa
     7381 ttgcaggact tgaccctgcc gggggagccg gactgctggc agaccttaaa acaattgcag
     7441 ctcacggaca tcatggtgtg ggagtcttaa ccgctgcaac agcccaaaat acagttggcg
     7501 tcaaaagttt ttacccactc acacccgaag cagtggtggc gcaactggat gcactacacg
     7561 aagatctggt gctggcagga gtcaaaacgg gcatgcttgg aaccggggcg attgcggaag
     7621 cgattgccga ctggttccat cgccactgga caagcccggt ggtggttgat ccagtactgg
     7681 tcgccagcag tggtcacctg ttgtttgacc ttgaagcaat cgatgtcatt cgcaaacgac
     7741 ttctcccact ggcaacagtg attactccga accttcccga agccgccaca ctgactgggc
     7801 gaaccgttga aacactgtct caaatggaag atgcggcccg tgaactttat acctatggtc
     7861 cccgctatgt tctgatgaaa ggtggacacc tgatgactga agccatggat gtcctctttg
     7921 atggcgagca gttccattat tttccgggaa cgcggcttga agtctatacc catgggacag
     7981 gatgcactct atctgccgcc ctggcctcac tgttagggag tggcttcact atcgtcgaag
     8041 ctgtaaagcg agccaaagcc tatgttcgcg ttgccatgca gcacacgctc tgcgtgggaa
     8101 aaggaaaaga attgctgggt catcttccgc tggacttact gcgggaacat aacacaacga
     8161 ttacctccga ttgatcaatc aaatggatac atttttgaaa ctcagcttct gttttgcctg
     8221 ggcctaaagg tcaaagttta tgtcgttttc gttccccaaa ctctttaaat acctgtcact
     8281 ggctgccttt cttggagtcg tggtctacct ggccatccaa ctcagctcca gaaataaccc
     8341 gctgcgtcct aaaaaaccaa ctgatctgtc ggtgcagctt gttggtgtct caaatgattt
     8401 tcggtatgtc catcgagacc aaaatgttga tcggctcatt ttgacggcgg caaaagatac
     8461 cgcctttacc aatgggcaac atcaactgga agaagtgaaa ttagaaacat ttggccagga
     8521 tggaaagccg cttggggtgt taacatcaaa aacagcagac tatgatagcc aggcccaggt
     8581 agccgttttt aaaaaagatg tcctggtcgt aacgaccgat gaactggtgg ttcgaacgaa
     8641 cgaaatgaag tatgaccagg cagccaatgt gacctcaact gaagccccag taacctttac
     8701 ccgcagaaat atcaccggaa cctgtgtcgg cgccaaactc gactccacgg caggtcgctt
     8761 tgtgatgaac cagcaggtca aaattacgat cgcccccgaa tcaacggctc cggcatcgcc
     8821 agttccgtca acaaccgctt cagccgcttc cccacctcag tcagcaccag atcaggataa
     8881 aaaagacaaa aaggacaaaa aaaataaaaa ggataaaaaa gataaaaagg acaaaaaagc
     8941 caaccgtgta aaagaaaccg gcaatcagcc gtctacggct tccaaaccgg cctcaacatc
     9001 tccggcgctc accgccgaaa taccaacgac catcactgga gaccacgctg aatactggaa
     9061 acctgaaaag caggtttacc tgaccgggaa tgccattatc ctgcagggcg gtaatgaact
     9121 ccgggcagat aaaatgaccg catttttgga tgataatcaa cggattcaga aacttgaaac
     9181 ccgagccaga tcatttctga aatcagccga aaaacagggg caagttgagt cccaggatat
     9241 ggatattacc tttaccagcg acggggatgt ccagtcagca cttgctacgg gcaacccggt
     9301 agtaaaatca tttgaaggac cagctccacg tgaagtaact ggtgaccgaa ttgaggtttc
     9361 atttgccaaa gtaaatgaag tcaatgcgtt acagc
//
LOCUS       DAOQDO010000008         9370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3849_length_9370_cov_3.097477, whole genome shotgun sequence.
ACCESSION   DAOQDO010000008 DAOQDO010000000
VERSION     DAOQDO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_9370_cov_3.097477"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKZ53_00365"
     CDS             <1..203
                     /locus_tag="PKZ53_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ38913.1"
                     /translation="PRLMVGIWPVTERVATTFWTAFYRNLLVEKQRPAAALRAAQLQV
                     MKEKKWASPYYWAAFGMYGDFR"
     gene            complement(306..1223)
                     /locus_tag="PKZ53_00370"
     CDS             complement(306..1223)
                     /locus_tag="PKZ53_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ38914.1"
                     /translation="MKRFAIFWAMFWFLQAGIAVNQPNDTTSRISLPAPQTPIIRTHA
                     VKLNVTNMDKALTFYTSQLGFQVADRSGFPNSVVLETNERVKLVLNRVKKLRKASPVD
                     TDASFTLQVNDLDQAMARMKSLKVPFAEKHPRKEGVGNAISIVDPFGRSISLMHQTIV
                     KVEPFQEPRLYNFGFTVPEMEPARQFYTQVLGFQVRSEKYLPLDLPLGHQDKSFAFML
                     HYRPGIRPVAGKPHQGAAFNTVVFETAQFDLAIQFLEQAKIQIIPDQSRNVTDQSPNA
                     TAEKWSQRRKLSFLDPFGNVSELVEELKQ"
     gene            complement(1335..2249)
                     /locus_tag="PKZ53_00375"
     CDS             complement(1335..2249)
                     /locus_tag="PKZ53_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ38915.1"
                     /translation="MILRSFPGTDYQAPETRRLLSLFGQETIIIHALTDQIAFAQHTA
                     PLSIKTTFQGCERYEINGHPVVVDQHSYLVINQDQPYASSIESIAPVESFCVFFRDDL
                     TQEVLSVFCKQAAWLVDHPESQDTQPPELFFQHRRPVDHLISPILANIHQRIQTNEIT
                     QSWLDEQYHFLIERLLQQHAHIQQEIEKLPMVRRSTRVELFKRLFRARDFIESCYAEP
                     ISLTDMAGAACLSRHHFLRLFTQVFHQTPHQFLTTVRLSHAWQLLATHRHTVTEVCFA
                     VGFENTSSFARLFKRHFGCSPHTRVLRS"
     gene            complement(2444..2980)
                     /locus_tag="PKZ53_00380"
     CDS             complement(2444..2980)
                     /locus_tag="PKZ53_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38916.1"
                     /translation="MPRLFLLLLAVVALVVGSGFLFQAATQETKPTPPKTCDELDKVD
                     YAPERLVGDKIVISTLDANSDEGKQVNSAEKLFSPHRTRWVTSKRPDTMRPGPWTTVL
                     FVEGNKANPGYLKLEFRNHASYGVDAKWLNEKLLFLQVWWGRVGSTDLILDVETNQVL
                     YCESASYLKLILPCDQKQ"
     gene            2981..3622
                     /locus_tag="PKZ53_00385"
     CDS             2981..3622
                     /locus_tag="PKZ53_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38917.1"
                     /translation="MTNTRWKGDGPRFEDNGLFLDELASHFLVGCPQCQNCALVRPIG
                     ERYPSKARVTCGACGLAQEQAFRFDDWQGPVVVTIGQKCGRCGAWLRRPPKILDQSPP
                     KRHSKMNCPGCQTSNVIEWTIHPLSQGRPVDCYFGHPLWFQTPCCSEILWAYNQPHLE
                     FLKAYVGATLRHRQPNHNQSLASRLPRWIKLAKHRDEVLRAILRMEVLMPPNV"
     gene            3795..7514
                     /locus_tag="PKZ53_00390"
     CDS             3795..7514
                     /locus_tag="PKZ53_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ38918.1"
                     /translation="MKHFQLSSLVCLILVCGSGLAWGQPSPGQPPRLKFKRITLEQGL
                     SSTLVRSVNQDHDGFIWFGTEGGLNRYDGNAIKVIRSKSNEPDTLASNYIDLIYQDQQ
                     KRLWIGTAGGGLHQFLRDQQFTLFRNEPRRAESLINNWVKTMVEDSQGQLWVGTDGGI
                     TIFDAHGKFVKSFQVDGTSARSLSHNYVKIIYIDRAGTLWVGTDGGLNRFDPADQGFT
                     RFQHNPGNPNTLSSNLVKAIFEDSAGRLWIGTDDGLNLFDRATQTVRRYYHTPTQPGS
                     LSNDTVTCILEGKAGQLWVGTDGGGINLYDPQTDQFSAVRYDPHDPYSLSSDRVTCFF
                     RDGAGSLWVGTYGGGVNFFDPLIQRFQVIQSDPLNPNSLSLNQVWSVWKDRLGAIWIG
                     TTGGGLNRLDPQTGQMTVFRAGPGQSTRLSHDEVLAIYEDRLGAIWIGTRSGLNRYSP
                     EKKGFVRFFHNPADPQSLSGNHVITFAEDLAGRLWIGTYGEGLNLYDPKQQRFTIFRP
                     DPTQPNSLCNNVVNYLYRSRDGMIWIATAGGLNRLDPVTLTFTAFRHDPANPKSLSHD
                     VVYSIREDNAGRLWVGTSSGLNLFDPQTGGFTVFSEADGFLNDTILGILTDETDHLWL
                     STKKGLVKFDPNQRQVRVFDMRDGLPSDEFNKNAFFQSPDGELLFGSTAGVVRFYPNR
                     LTSNSFVPPVVITSFRIFDKPSEPYTGDLLTHPMAQGDSAVVVLPYDQNFFSFDFAAL
                     NFILPEKNQYTYRLEGLDPRWIEAGTRHTASYTAVPPGSYVFRVKASNNDGIWNEREL
                     TVRLTITPPPWQTWWARLLYVTGLVGGGFWLVWSRVQKFRERAELREVRLRVKAAEAE
                     AQVAHLQAEAAEQLRRQNEALARKNTELEASQRQADRIFSTLTEALEGMVLDGKYRLD
                     EKIGIGGFGAVFQATHLGLNRKVAVKVFRPLPGNDSAEQLERFRREGMSATKVNHPNA
                     IAILDSAISEEGIAYLVMEFLVGHTLATELHNRKRLSLKRCAEILTPVCQVLDEAHRA
                     GIIHRDIKPENIFLHQGSQGEIIKLLDFGIAKLTGTEATSLGNLTLTGNLIGTPMYMS
                     PEQLRYEPYDGKADVYSLGVTLYEMLCGRTPFRSTSNNVVEIILKQMRDDPPPMRSAN
                     PEIPFEVEAVVRKTLDKDPANRPSPLELANEFAAAVAGYLVGVLPDSPTETNLRIEPD
                     EDELLENHPTLTTAEVEENAPTVAFQRPETPEEPG"
     gene            complement(7875..8927)
                     /locus_tag="PKZ53_00395"
     CDS             complement(7875..8927)
                     /locus_tag="PKZ53_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ38919.1"
                     /translation="MRKLLSILLPIAGIVFAIYWVMISAQRPTPAKPLNPPAQNTFLK
                     TIAGAGIIEAASRNINLAPPIPGQVTQIFVKENDFVKQGASLYRIDDRELRAKLATAE
                     ANIERARANIETVQTEIGNSEAAQQSAQANISVLKAQLQDTQQILDRNAQLVKSGDLS
                     TRIYDSSVAARDAAKARVEQAEAQLAQTAAQLRSVKARLLQAEADLKVLQAQRQELMV
                     NLDRLEVKAPQDGRVLQINIRLGEFVSSTPTTPPMLFGETDYLQVRVDVDEINASRVK
                     PGLPAEAQLKGDTAKKIALEFVRIEPYITPKKSLTGDNTERVDVRVLQVIYRFRSPDF
                     PVYVGQQVDVFIDASK"
     gene            complement(9098..>9370)
                     /locus_tag="PKZ53_00400"
     CDS             complement(9098..>9370)
                     /locus_tag="PKZ53_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ38920.1"
                     /translation="QLSGGQQQRVAIARALVAEPRLIICDEPTASLDGETGKMVMEMF
                     RKTALSENRAIVVVTHDNRIFGYGDRIAEMLDGRILSIHENPDHVT"
BASE COUNT         2274 a         2267 c         2490 g         2339 t
ORIGIN      
        1 caccacggtt gatggtcgga atatggccag tgaccgaacg cgtggcaacg actttttgga
       61 ccgcgtttta ccgaaatctt ctggttgaaa aacagcgtcc agccgctgcc ctgcgtgcgg
      121 ctcaattaca ggtgatgaag gaaaagaaat gggcttcacc ctattattgg gctgcctttg
      181 gaatgtatgg ggatttccgg taacgcacgg tctcacaatc ttcaaaaggc taacttattg
      241 aaaaagttat gtttccctca atactaattt gtacgaggcg ctgaatcagc accctacaaa
      301 tacctttatt gttttagttc ctcgaccagt tcagacacat tgccaaatgg atcaagaaag
      361 ctcagctttc gccgctgaga ccacttttca gccgtggcgt taggtgactg atcggttaca
      421 ttccgcgatt gatccgggat tatttggatt tttgcctgtt ccagaaattg gattgccaga
      481 tcaaattgag ccgtttcaaa tactaccgtg ttaaaggcgg caccctggtg tggtttgccg
      541 gcaacaggtc ggatccctgg ccggtagtgc aacataaagg caaaactttt atcctggtgg
      601 cccagtggca ggtcgagcgg gaggtacttt tcactccgaa cctggaaacc caacacctgc
      661 gtgtaaaact ggcgggcggg ttccatctca ggcactgtaa atccaaagtt atacaggcgt
      721 ggttcctgaa atggctcaac tttgacaatt gtttgatgca tcagcgaaat cgaacgtcca
      781 aatgggtcca cgatggaaat tgcgttgccg acaccttctt ttcgagggtg tttctcagca
      841 aaaggaacct tcagtgattt cattcgggcc attgcctggt cgagatcgtt gacctggagt
      901 gtaaacgaag catccgtatc aaccgggctg gccttgcgga gtttcttcac ccggttgagt
      961 accagtttga cccgttcatt ggtttccaga acaaccgagt tgggaaagcc actccggtct
     1021 gcaacctgaa agccgagttg gctggtgtaa aacgtgagag ctttgtccat gttggtgaca
     1081 ttgagcttga cggcatgggt tcgaatgatc ggagtttggg gagctggaag tgagatcctg
     1141 ctggttgtgt cgtttggttg atttactgca attccagctt gaagaaacca gaacatcgcc
     1201 cagaaaattg caaatcgctt catacgcaac accttgtgga gaatgaattg gagtgcctgg
     1261 tttttcagtt tcaatcccca agtatcttca actcctggct ggctggctgt gcgaaaattg
     1321 ctttttttac tttctcagga acgaagaacg cgtgtgtgtg gcgagcatcc aaagtggcgt
     1381 ttaaacagac gagcaaacga acttgtattt tcaaagccca ccgcaaaaca aacttcggtg
     1441 acagtgtggc gatgagtggc aaggagttgc caggcgtgac tgagacggac agtcgtcaaa
     1501 aactgatgag gtgtctggtg aaacacctgg gtaaacaatc gcaagaaatg atgacgtgac
     1561 agacaggcag ccccggccat gtcggtcaac gaaattggtt cggcataaca ggattcgatg
     1621 aaatcacggg cccgaaagag tcgtttgaac aactcaaccc gagtcgagcg gcgaaccatc
     1681 ggcagctttt caatttcctg ttgaatgtgg gcgtgttgct gcaacaatcg ttcaatcaaa
     1741 aaatgatact gctcatctaa ccagctttgg gtgatttcgt tggtttggat ccgctggtga
     1801 atgttcgcta aaattggtga aatgaggtga tccactggcc gtcggtgctg aaaaaaaagc
     1861 tcaggaggct gtgtgtcctg gctttcagga tggtcaacca gccaggctgc ctgtttgcag
     1921 aacacagaaa gtacttcctg ggtcagatca tcacggaaga agacacaaaa tgattccacc
     1981 ggagcaattg attcaattga actggcatag ggttgatcct ggttgatgac gagataagaa
     2041 tgctggtcaa ccacaaccgg atgtccattg atttcatacc gttcacaccc ttgaaacgtc
     2101 gttttgattg aaagtggtgc tgtgtgctgg gcaaacgcaa tttgatcggt cagggcatgg
     2161 ataatgatcg tctcctgacc aaacagagaa aggagtcgcc gggtttctgg tgcttgataa
     2221 tcagttccag gaaatgaacg caatatcatg ccaatgaggg ttcagggttc agggttttcg
     2281 aaaaaagtca ctttgtcatc tatcgtcttt cggaaaaaga tttcccctgg gaacgccggc
     2341 atcttgcggg taactgattg gatatgaacc acttggtgcc agcaggatgc cagcgttccc
     2401 aggaaatatt tatctgaaaa gctagagtca cccagtcacc tcatcattgc ttctggtcac
     2461 aaggcaagat cagtttcagg tagctggcag actcacaata cagaacctga ttggtttcaa
     2521 catccaggat caagtcggtg cttccaacgc gaccccacca gacttgcagg aagaggagct
     2581 tttcgttgag ccatttggca tctacgccgt aggaagcatg gttgcgaaat tcgagtttga
     2641 gatagcccgg attggctttg tttccttcga caaacaggac tgtggtccag ggaccaggcc
     2701 gcatggtgtc aggtcgtttt gacgttaccc atctggttcg atgtggcgag aagagttttt
     2761 ctgcgctgtt gacttgtttt ccttcgtcag aattggcatc aagggtggaa attacgattt
     2821 tgtcgcccac cagtctttca ggcgcatagt caactttgtc taactcatca cacgttttcg
     2881 gaggcgttgg tttggtttcc tgcgtcgccg cttgaaaaag aaaacctgaa ccaaccacca
     2941 gcgcgacaac ggccagcaaa agcaaaaaga ggcgtggcat atgacaaaca ctcgttggaa
     3001 aggggatggt cctcgatttg aggataatgg cctgttttta gatgaactgg catcgcactt
     3061 tctcgtgggc tgcccacagt gtcagaactg tgcgctggta cgtccgattg gtgaacgcta
     3121 cccgtcaaaa gccagggtga cctgtggtgc ctgcggtttg gctcaggaac aggcgtttcg
     3181 gtttgacgac tggcagggcc cggtggtggt gactatcggt cagaaatgtg gccggtgtgg
     3241 cgcctggttg cgacgaccac caaagattct ggatcagtct ccgccaaaac gccactcaaa
     3301 aatgaattgc cccggatgtc aaaccagcaa cgtcattgag tggaccattc acccgctttc
     3361 acaaggaaga cctgtagatt gctattttgg acatccgttg tggttccaga ctccctgttg
     3421 cagtgaaatc ctgtgggcgt acaaccagcc acacctggaa tttttaaaag cctatgtcgg
     3481 ggcaactctg cgtcaccgtc aacccaatca caaccagtcc ctggccagcc gattaccgcg
     3541 ctggatcaaa ctggccaaac atcgggatga agtgctgcgg gccattctgc gcatggaagt
     3601 gttgatgccg ccaaacgttt gatccaaaaa agccttgttg aatttggaaa actggtgttc
     3661 actgggaatc cactgctggc acctgccttt gatctctgtt tcaatctgaa aaaaccgaaa
     3721 gtgaatagcc atccccagac tgaggattcc cccgacctca gtgactcatt gcctgctcgg
     3781 aaaggttacc tggcatgaag cattttcaac tcagcagcct ggtctgtctg atcctggtgt
     3841 gtggttcggg attggcctgg gggcagcctt cacctggtca gccgccgcgc ttaaaattca
     3901 agcgcattac tctggagcag gggttgtcaa gcactctggt gcgctctgtc aaccaggatc
     3961 atgatggatt tatctggttt ggaactgaag gtgggctcaa tcgctatgat gggaatgcca
     4021 taaaagtaat tcgctccaaa tccaacgaac ctgatacgtt agcgagtaat tacattgatt
     4081 tgatttatca ggaccagcag aaacggttgt ggatcggcac cgccggcgga ggcttgcacc
     4141 agtttctgag agatcagcaa tttacccttt ttcgcaatga gccacgccgg gccgagagtc
     4201 tgatcaataa ctgggtcaag actatggttg aggatagcca gggacagctc tgggttggaa
     4261 ctgacggtgg aataaccatt tttgatgctc acgggaagtt tgtgaaatca tttcaggttg
     4321 acggtacctc tgcacgcagc ctgagccata actatgtcaa aataatatat attgatcgcg
     4381 ctggaactct ctgggtcggc accgatggcg gattaaaccg ctttgatccg gctgatcaag
     4441 gatttacccg gtttcagcat aatccgggca atcccaatac cttgagtagc aatctggtca
     4501 aagccatttt cgaggattct gccggtcggc tctggattgg aaccgatgat ggattaaatc
     4561 tgtttgaccg ggcaactcag actgtccgcc ggtattacca cacgccaaca caacctggaa
     4621 gtttaagtaa cgacaccgtg acctgtattc tggaagggaa agccggtcag ctttgggtgg
     4681 gaacagatgg tggcggtatc aatctatatg atcctcagac agatcaattc tcggcggttc
     4741 gatatgatcc tcacgaccca tacagtctga gcagtgatcg ggtgacctgt ttttttcgcg
     4801 atggtgctgg atcgctctgg gttggtacct acggcggcgg agtgaatttc tttgacccgc
     4861 tgattcaacg gtttcaggtg atccagtcag acccgcttaa cccaaacagc ctgagcctca
     4921 atcaggtctg gtcagtgtgg aaagatcgtc ttggcgccat ctggattggg accaccggcg
     4981 gcggactcaa ccggttggac ccacaaactg gtcaaatgac agtttttcgg gcaggcccag
     5041 gccaaagcac ccgtctcagc cacgatgaag tcctggccat ctatgaagat cgtctcggtg
     5101 ccatctggat tgggactcga agcggcttga accgatacag ccccgagaaa aagggctttg
     5161 tacggttttt ccacaatcca gccgatcccc aaagtctgag tggcaatcac gttattacat
     5221 ttgccgagga tctggccggt cggctttgga ttggcaccta tggcgaaggt ctcaacctgt
     5281 atgatccaaa acaacagcgg tttactattt ttcgacccga cccgacgcaa cccaatagtc
     5341 tttgtaataa cgttgtgaat tatctatatc ggagccgtga tgggatgatc tggattgcaa
     5401 ctgccggcgg gctcaaccgc ctggatccag tgacgctcac ctttactgct tttcggcacg
     5461 acccggcaaa tcccaaaagc ctcagccatg atgtcgtgta ttccatccgc gaagacaatg
     5521 ctggtcggtt gtgggttgga accagtagcg gcttaaatct gtttgaccca caaaccggag
     5581 gatttacggt tttttctgaa gcagacggtt ttctcaatga caccatcctg ggcattttga
     5641 ccgatgaaac ggatcattta tggttgagta ccaagaaagg actggtgaaa tttgacccca
     5701 atcaacggca ggtgcgtgtt tttgatatgc gtgatgggct gccgagtgac gaattcaata
     5761 aaaatgcctt ttttcaaagc cctgacggag aattattgtt tggcagcacc gccggggtgg
     5821 ttcgattcta tccaaaccgg ttgaccagta attcatttgt tccgccagtg gtcatcacgt
     5881 cgtttcgaat ttttgacaaa ccgtctgagc cgtatactgg tgatttactg acccatccga
     5941 tggcacaggg tgattcggcg gtggtggtct tgccctatga tcagaatttt ttcagtttcg
     6001 actttgctgc ccttaatttt attctgccgg agaaaaatca gtatacctac cgtctggaag
     6061 ggcttgaccc acgctggatt gaggccggaa cccggcatac agcgagttac acggcggtgc
     6121 ctccgggaag ttatgtgttt cgggtgaagg cttcaaacaa tgatgggatc tggaatgaga
     6181 gggagttgac ggttcgattg acgattacac ctccgccgtg gcagacctgg tgggcgagat
     6241 tgctctatgt cactggtctg gttggtggtg gattttggct cgtgtggtcc cgagttcaga
     6301 aattcaggga acgggctgaa ctccgcgaag tccggctccg ggtcaaagcc gccgaagccg
     6361 aagcccaggt ggctcatctc caggctgaag ctgctgaaca actgcgccgc caaaatgaag
     6421 cgctggcgcg aaaaaatact gaactcgaag cctcgcaacg ccaggctgac cggattttct
     6481 caaccctgac cgaagccctc gaaggaatgg tcctggacgg gaaataccgc ctggatgaaa
     6541 agatcgggat tggcgggttt ggcgctgtct ttcaagccac gcatttgggg ctcaatcgaa
     6601 aagtggcggt caaagttttt cgccccttgc caggaaatga ttcggctgag caactggagc
     6661 gatttcgacg cgaagggatg tcagccacca aagtcaatca tcccaatgcg attgcaattc
     6721 tggattctgc catttctgaa gagggaattg cctatctggt gatggagttt ctggttggtc
     6781 atacgctggc aaccgaactt cacaaccgaa agcgattgtc gctcaaacga tgcgccgaaa
     6841 ttctgactcc ggtgtgtcag gttctggatg aggcccatcg ggccgggatt atccatcgcg
     6901 acatcaagcc ggaaaatatc tttctgcacc agggaagcca gggcgaaatc attaaactgc
     6961 ttgattttgg aattgcgaaa ctgaccggaa ctgaagccac cagcctgggc aatctgaccc
     7021 tgactggaaa tctgattgga acgccgatgt atatgtcacc ggaacaattg cgctatgaac
     7081 cctatgacgg caaggcagat gtgtacagtc tgggggtcac tctgtatgaa atgctttgtg
     7141 gccgaactcc gtttcgttcg accagcaaca acgtggttga aattatctta aagcagatgc
     7201 gggatgaccc accaccaatg cggtcagcca atccagagat tccatttgaa gttgaggcgg
     7261 ttgttcgcaa aaccctggat aaagaccccg ccaaccgacc ctccccgctt gaactggcca
     7321 acgaatttgc ggctgctgtg gctggctatc tggttggggt attacctgat tccccaaccg
     7381 aaaccaacct ccggatcgag cctgacgagg atgaattgct tgagaatcac ccaaccttga
     7441 cgacggctga agttgaggag aatgccccga ccgttgcttt ccaaagaccg gaaaccccag
     7501 aggaaccggg ctgaagacat tgggctgaag acatcgggct cggggctgag ggccataagc
     7561 gccaggggat aaaggagatg accccaatct aataccaatt agggttcagg gtatcgggtt
     7621 cagggttcag ggaaatacaa tttcttcaat accttagcct tcccgtaata cgaggacttc
     7681 attgcaaaat ggtataaaca aggagacacg ggcaataact tgttctcaat gtgccaaagt
     7741 ttccttcatc ttggtggctt atggggttgg ggggacgttc cgggaaaaga caaaaaggac
     7801 gaaaaccgga tttcgaaaaa cctgaaccct gaaccctgaa ccctgaaccc tgagccctga
     7861 accctgaacc ctgattattt gctggcatca ataaacacat ccacctgctg gccaacgtag
     7921 accggaaagt ctggcgagcg gaagcgataa atcacctgca gaacacgcac atccacgcgt
     7981 tcggtgttat cgccagtcag gctctttttc ggtgtaatat agggctcaat tctcacaaat
     8041 tcgagggcaa ttttcttggc cgtatcgcct ttgagttgag cttcggcagg cagtcccggc
     8101 ttgacccgcg aggcattaat ttcgtctacg tctacccgaa cctgcaaata atcggtttcc
     8161 ccaaataaca tcggaggcgt ggtcggagtt gaggaaacaa attcacccag ccgaatattg
     8221 atttgcagca cacgaccatc ctgaggggct ttaacttcca ggcgatcaag attgaccatc
     8281 agttcctggc gttgcgcctg gagcactttg agatcagctt cagcctggag caggcgcgct
     8341 ttgacgctgc gaagttgagc cgccgtttgt gccagttggg cttcggcctg ttcgacccgt
     8401 gctttggcgg catcacgagc cgccacgctt gaatcataaa tccgggtgga taaatcccct
     8461 gatttcacca gttgcgcatt ccgatccagg atttgctgag tgtcttgcaa ttgagctttg
     8521 aggactgaaa tgttggcctg ggcgctttgc tgggctgctt cagaattacc gatttcggtc
     8581 tgaactgtct cgatattggc tctggcacgt tcgatattgg cttcagccgt ggccagtttg
     8641 gcgcgcagtt cccggtcatc aatccgatag agactggcac cctgtttcac aaaatcattt
     8701 tccttcacaa aaatctgagt cacctggcct ggaattggcg gggctaaatt gatgttccgg
     8761 ctggcggctt caatgattcc agcaccggca attgttttga ggaatgtatt ttgcgccgga
     8821 ggattgagtg gtttcgctgg ggttggtcgt tgggctgaaa tcatgaccca atagatggca
     8881 aagacaattc cagcaatcgg taacaggatg ctgagtagct ttcgcataga gattccttcg
     8941 gaaaattggt gactggtgtt agggcttgtc agtaccacct gcgtgagcgg gtggggttga
     9001 aagccagccg acccacccac tgacgcgggt ggtactgaca tatcgattct tttgatttgt
     9061 ccccccgcaa aatccggtag aagcttgaac tctgaactca cgtcacatgg tctggatttt
     9121 cgtgaattga aaggatgcgt ccgtccagca tttcggcaat tcggtcacca taaccaaaaa
     9181 ttcggttgtc atgggtgacc accacaatgg cacggttttc gctcaaggcc gtcttgcgga
     9241 acatttccat caccattttt ccggtttctc cgtcaagact ggccgtgggt tcgtcacaaa
     9301 tgatgagccg tggttcggcc accagcgccc gggcaatcgc aacgcgttgt tgctggccgc
     9361 cagaaagctg
//
LOCUS       DAOQDO010000009         9201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_3976_length_9201_cov_3.198666, whole genome shotgun sequence.
ACCESSION   DAOQDO010000009 DAOQDO010000000
VERSION     DAOQDO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_9201_cov_3.198666"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2384
                     /locus_tag="PKZ53_00405"
     CDS             <1..2384
                     /locus_tag="PKZ53_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ38921.1"
                     /translation="VKAISYQQGRCQLKAVTFSDDVVYSLAATYLDGKPTLWLGTNSG
                     LISFRSENWTHYSTANSKLPNNQILHLYTTGEKNDPSQYLWIGTRNGGAIRVNPTDPQ
                     GQWMVFSANSQPAIPNNSIHQIQVDRQGRVFLMTNKGVACLIPPEGAISNQTEFTTCT
                     FLDEDGLPSNECTQGAIRDSQGRIWAATVNGVAVFSPISQKFDQTPKPLYIERIIVNG
                     EAQPPNVTYLNLQHDQNNLSFEFSLLNFQRGDSTRYRVQLSGYDPFPSGWGRDYKAVY
                     TNLAAGNYTFLVWGKDTFENITGPISVAVSIKPSVWKTWWAYGLYVVILAGTGIGTYT
                     WRVTILRRRQEKRVRFLRQLLESTRIINSKLELTTVLQIITDEAARLVNGEPGGIGLI
                     ENGNVVFHRVWCRDHWEDLTVSFPPGQGIAGRVAEQGMAVIINEPTGNDELLYPDLVE
                     KFDIHGLLNVPIFSRTGKVVGVLDIRRPKGRAPFTEADSHILEALSNQAAVAIENAGL
                     YGELEEKNLLIGESMRELQKLYHREQEVTRSLKQLDQMKSNFLAITAHEMRTPITVLS
                     GTHEILLDQLVGTLNAEQRELLQTCRIMTERMLTTIEDVQEMLRITKGQFNLSPCSFD
                     LNLTVQDIVNELSDVTTDRNLKINIISAGECEIYADPDKIRQAIWNLIQNAVKFTPNG
                     GRINLHLEQTEDFCHLAVDDSGIGIEPEEHDRIFDCFYAGADATQHKSGKYEFMARGL
                     GLGLAIVRSYIEAHGGRVSVASKGRNQGTCFHVWLPIRQQIQEPVESLVEDGK"
     gene            2796..3062
                     /locus_tag="PKZ53_00410"
     CDS             2796..3062
                     /locus_tag="PKZ53_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ38922.1"
                     /translation="MRSLATKKVVPEMPPEAKARRIFGDVVVEITVSTQGEVLFARCV
                     AGHQLLQEAALTAARQWKFKSPLVAEYPTHWVTGVLTFRFPPSS"
     gene            complement(3157..4359)
                     /locus_tag="PKZ53_00415"
     CDS             complement(3157..4359)
                     /locus_tag="PKZ53_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ38923.1"
                     /translation="MDFSIAQDTQKLLAEIREFIQAEVFPLEPQVLAGRFHLMDDQLE
                     AKRAEVKARGWWSPFIPKELGGMGLRLMEYAHISEELGRSMIGHYLFNCQAPDVGNME
                     VLMHYGTPEQKQHYLYPLAEGKIRSCFSMTEPGYPGSNPVWMGTTAEKDGEDYVINGR
                     KWFTSSADGAAFAIVMAVTNPDAESPHRRASQILVPIDTPGFNLVRNVSIMGEPGGAW
                     LSHAEISYENCRVPQSNRLGPEGAGFSIAQERLGPGRIHHCMRWIGICERAFDLMCSY
                     AAKRELSPGKPLGTQQIVQAWIAESRAEINAARLMVLQAAWKIDQTGTKSAIEEISVI
                     KFYVAGVLLRVLDRAIQVHGALGITDDTPLAFWYRHERGARIYDGADEVHKSSVAKRV
                     LRGYGVKV"
     gene            4746..>5400
                     /locus_tag="PKZ53_00420"
     CDS             4746..>5400
                     /locus_tag="PKZ53_00420"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNJ38924.1"
                     /translation="MLVLSRIALFLTFGFTLCISPASLFAQSFNTNSGNDDVKFAGTI
                     ESLPSNGSLLGDWKVAGKTVRVTSSTKIEQERGQAAVGAEVEVEAVPQSDGSFLASEV
                     KVIRGSGGGSGSEVKFYGTISSLPASSDLRGNWVVDGRNVMVTASTSLDQSRGSFAVG
                     VRVEVEGYAQSDGSVTASSIHVDDNSTGGGSEVKFYGTINSLPASSDLRGNWVVDGRN
                     "
     assembly_gap    5401..5500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5501..6546
                     /locus_tag="PKZ53_00425"
     CDS             <5501..6546
                     /locus_tag="PKZ53_00425"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNJ38925.1"
                     /translation="RGSFAVGVRVEVEGYAQSDGSVTAKSIHVDDNSTGGGSGGMYIK
                     FYDRIQTLPASADLIGTWQIGGRSVQVTSSTQLDRSRGNFAVGVLVEVEGSQQADGTV
                     TAKEIHVESSGGGGSSSGSYFEFYGVIETQPAGTGVVGDWTIAGRTVRVTSSTSIETR
                     NGTLQVGALVEVKGVVDSAGVITASRIEVKSSSSTGGGSTQYSQFYGNIEALPTSSDQ
                     LGEWRISGRTVTVTANTYIDREHGPAVVGAYVEVEGLVQADGSFTATKIEIKEKTGSN
                     GRSSFHGIVETLPDTSNLVGDWKVSGRIVRVTTSTKVSQRGGSISVGTRVEVKGSENS
                     DGSITATRIKRDS"
     gene            complement(6891..7778)
                     /locus_tag="PKZ53_00430"
     CDS             complement(6891..7778)
                     /locus_tag="PKZ53_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ38926.1"
                     /translation="MTDRMKLLIAYDGSECADAAIDGLLRAGLPEEAEAFVLTVSEMG
                     LAVPPPSIGMVETSFSSAIQDATEEALRIAQTGLDRVKTLFPRWEVTAEGRFGSPGRE
                     ILEKAVEFNADLIVIGSHGRSAVGRLVFGSVSTQVVTQAHCSVHVVRPGKAAVTDPVK
                     LVIGIDGSKGSEIAVETVATRRWPAGTEVHLVTSAGPFDLYGVAYDEKMGFASKLQAM
                     ARRRLEEAGLQTISVIMEGGPKEVLMEEAEQIGADCVFVGSRNLSRFDRWLLGSVSTA
                     VTTRAHCTVEVVRKHGAEE"
     gene            complement(8080..8673)
                     /locus_tag="PKZ53_00435"
     CDS             complement(8080..8673)
                     /locus_tag="PKZ53_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ38927.1"
                     /translation="MKVLIADDDEVTRTLVQLSLERAGYEVMVTCDGEEALAALKADD
                     TIRIVVSDWEMPRLNGVELCQQVRAFRHNDYTYFILLTAVHTDDDHYYQAMEDGVDDF
                     LLKTMATKTLRLRLKVAERILGFTRQINQLKELLPICCYCKRIRDDNDYWQQIDEYIH
                     DQTGTDFSHGICPNCYDSRVKPMLDRLLPPKPSDPAQ"
     gene            complement(8788..>9201)
                     /locus_tag="PKZ53_00440"
     CDS             complement(8788..>9201)
                     /locus_tag="PKZ53_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011043177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38928.1"
                     /translation="VVEIQRIFAEGLRKAAAGADVIVLCIGENAYAESPGNTRDLALP
                     KEQEALALLSDPDLAEFAVGLDTFWATNEQIETGIEKAVNFVEEHPRSLYCPIVRTKL
                     NQMLEYLKSSDRLLSPKLQNYFKKFLDEKGAIQSR"
BASE COUNT         2249 a         2368 c         2261 g         2223 t
ORIGIN      
        1 tggtgaaagc catcagctat cagcaaggac gctgtcagct gaaagctgta accttttccg
       61 atgacgtggt gtattcgctg gccgcaacgt accttgatgg aaaaccaact ctctggctgg
      121 gcaccaattc cgggctgatt agctttcgtt cagagaactg gacgcactat tcaaccgcca
      181 attccaaatt gccaaacaac cagattttgc acctctacac caccggggag aagaatgatc
      241 cttcgcaata cttatggatt ggaactcgta acgggggcgc cattcgggtc aatccgactg
      301 acccgcaggg acaatggatg gttttttccg ccaactcaca gccagccatc cccaataatt
      361 ccattcatca aatccaggtt gaccgtcagg gtcgcgtctt tctgatgacc aataaaggag
      421 tggcctgcct gatccccccc gaaggcgcca tctcaaatca gactgagttt acgacctgta
      481 cttttttgga tgaagacggc cttcccagca atgagtgcac ccagggcgcc atcagagaca
      541 gtcagggacg gatttgggcg gcaacggtaa atggcgtggc tgttttttct ccaatttctc
      601 agaagtttga ccaaactcca aagccactct atatcgaacg aattattgtc aatggtgagg
      661 cccagccgcc caatgttacc tacctgaacc tgcaacacga tcagaacaac ctgtcctttg
      721 agttttcgct gttgaacttt caacgtggcg atagtacgcg ctaccgggtt cagctcagtg
      781 gatacgaccc gtttccttcc ggctggggac gtgactataa agcggtctac accaatctgg
      841 ccgctggcaa ctacactttt ttggtctggg gaaaagacac ctttgagaac atcactggac
      901 ccatctcggt tgccgtttcc attaaaccgt cagtctggaa aacctggtgg gcatatgggc
      961 tctacgttgt gattttggcc ggcaccggca ttggaaccta tacctggcgg gtgacgatcc
     1021 tgcgccgtag acaggaaaaa cgagtccggt tcctccgcca attgcttgaa agtactcgca
     1081 ttatcaactc caagctcgaa ctgacaaccg tgcttcaaat catcaccgat gaagcagccc
     1141 ggctggtcaa tggcgagcct gggggaattg gattgattga aaatgggaat gttgtgttcc
     1201 accgagtctg gtgccgcgat cattgggaag acctcaccgt cagttttcca cctggtcagg
     1261 gcattgcagg tcgggtcgcc gaacaaggca tggcggtgat tatcaacgaa ccgaccggga
     1321 acgacgaatt actctatccg gatctggttg aaaaatttga cattcatggc ttgctcaatg
     1381 ttccgatctt ttcgcgcacg ggaaaggtcg tgggggtgct cgatattcgg cgccccaagg
     1441 ggcgggcacc gtttactgag gctgattctc atattctgga ggcattatcc aaccaggccg
     1501 ccgtggccat tgaaaatgca ggtctttatg gcgagcttga ggaaaaaaat ctgctcatcg
     1561 gcgaatcaat gagagaactc caaaagctgt atcaccgcga acaggaagtt acccgcagtc
     1621 tgaaacaact cgaccagatg aaatctaact ttctggcgat caccgcccac gaaatgcgca
     1681 caccaatcac tgtgttgagc ggaacgcacg aaattctact cgatcaactg gtcggcacct
     1741 taaatgctga acaacgggaa ttgctccaaa cgtgccggat tatgaccgaa cgcatgctga
     1801 ccacgattga agacgtccag gaaatgctgc gcatcaccaa agggcaattc aacctttccc
     1861 cctgtagctt tgacctcaat ctgacggttc aagatatcgt gaatgaactc tcagacgtca
     1921 ccactgatcg gaatcttaaa atcaatatta tttcggcggg tgagtgcgaa atttatgctg
     1981 atccagacaa aatccggcag gcaatttgga acctgattca aaatgccgtc aaatttacgc
     2041 caaacggagg gcgcatcaat ctccacctcg aacaaaccga agatttctgc catctggcag
     2101 ttgatgacag tgggattgga attgaacctg aggaacacga ccggattttt gattgctttt
     2161 atgccggggc cgatgctacc cagcataaat caggaaaata tgaatttatg gcacggggat
     2221 tgggattggg gctggccatt gttcgcagct atatcgaagc ccacggcgga cgcgtctcgg
     2281 tggcctccaa aggtcgcaac cagggaacct gcttccacgt ctggctgcca atccgacagc
     2341 aaatccagga accagtcgaa tcgctggtcg aggatggtaa gtaattgaat tcaatacaaa
     2401 tttgggctga gggcatcggg ccaggggaaa tacaaatttc tcaacagctt aacctttgat
     2461 tgattcattt cgtgtatttc gtgtgtttcg tggttaaaat gtctggaaat tttcagcgaa
     2521 gtacttatga ggtcactatg acgagcaatg ccctgttgag aaatcaactt tggttcacct
     2581 tctgcctgtg cattgcagta ggactcagtt cttactattc gggttcagtc tttggttcta
     2641 gccttcgtcc actggatgga caaaccgcaa acaaatcaaa agtcggaccc gtgtctcaaa
     2701 acaagaagaa atccactgtt cgtacttctt caacttcaga accagattca gcacccgcac
     2761 cgctaccgca aaaaattcgg cgcagtgaag gtgtcatgcg aagcctggcc accaaaaagg
     2821 tcgttcccga gatgcctccc gaagccaaag ctcggcggat tttcggtgat gtggtggtgg
     2881 aaatcaccgt ttccacccag ggagaggtgc tctttgccag atgtgttgca ggtcaccagt
     2941 tgctccagga agcagcctta accgcagccc gtcaatggaa gttcaaatcg cctctggtgg
     3001 ctgaataccc aactcattgg gttacaggag ttttaacctt tcgatttccg ccatccagtt
     3061 aactgggcaa gatgggagcg ccggcatcct gcccacatgg atttcaattt caaccgattg
     3121 cgggcgagac acccgcgctc ccaggtgaac gctgaactaa accttgaccc catacccacg
     3181 caggacccgc ttggcaactg acgatttatg tacttcatcg gccccgtcat agatgcgggc
     3241 gccacgttcg tgccgatacc agaaggccag cggtgtgtca tcggtgatgc caagcgcccc
     3301 gtgaacctga atcgcccggt ccagcacccg caagagcact ccggcgacat aaaatttgat
     3361 caccgagatt tcttcaatcg ccgatttggt gccagtctga tcaattttcc aggcggcttg
     3421 taagaccatc aagcgggccg cgttgatttc agcccggctt tcggcaatcc acgcctgcac
     3481 aatttgctgc gtgccaagtg gtttcccagg ggaaagctcg cgtttggcgg catagctgca
     3541 cattaaatca aacgcccgtt cacagatccc gatccagcgc atacagtgat ggattcgacc
     3601 tggtccgagt cgttcctggg caattgaaaa accagcacct tccggaccca gccggtttga
     3661 ttgcgggacc cggcagtttt cataactgat ttcagcatgg ctgagccacg cgccacccgg
     3721 ttcccccata attgacacgt tacgaaccag attaaagcct ggtgtatcaa tcggtaccag
     3781 aatctgactg gcacgccgat gtggactttc ggcatctgga tttgttaccg ccattacaat
     3841 ggcaaaagct gccccgtcag ccgacgaggt aaaccactta cggccattga tgacatagtc
     3901 ttcgccatct ttttccgccg tggtgcccat ccagaccgga ttggagccgg gatagccagg
     3961 ctcagtcatt gaaaagcaac tgcggatttt tccttcggcg agcgggtaca ggtagtgctg
     4021 cttttgttcg ggcgtcccgt aatgcatcag cacttccata ttgccgacat ccggggcctg
     4081 acagttaaag agatagtgac caatcatact gcggccaagt tcttcgctga tatgggcata
     4141 ctccatcaag cgaagtccca taccacccag ttctttcggg ataaagggag accaccagcc
     4201 tcgggctttg acttcggcgc gtttggcttc gagctggtca tccatcaaat gaaagcggcc
     4261 agccagaacc tgcggttcga gtggaaaaac ctcggcctgg ataaattcac gaatttcagc
     4321 cagtagcttt tgagtgtctt gagcaattga gaagtccaca acagttgtcc tttataaata
     4381 aagatgtcga aggggctgtt agaaagtagc caaactctac ttgaaaaaat ctcaatcacc
     4441 aagcacaacc tcacttccgg caaagagttg gcaaaaaact gagaaaagtt tcataaattt
     4501 atcttctgtt tatctttccc tctctattat ctcgctcagt gcctggcttt gaggttttct
     4561 tcttttttct gcggcatcgg agacctgcag ccaggctctt gtttcaattg actaccttcc
     4621 tcaatcccac caataacttc cacaatgaga cgtagcactt gcccctaacg tcgagttcct
     4681 tgtggagtga caaatccatt aacatcgatt tgttatatat gcagcacctt ttggaggaag
     4741 ctgaaatgct ggttctgtct agaattgcgt tatttctcac ctttggattt actctttgta
     4801 tcagtcctgc ctctctattt gcacaatcct ttaatacaaa cagcggtaat gacgatgtga
     4861 aatttgccgg gacaattgaa agcctcccga gcaacggaag tttgcttgga gattggaaag
     4921 ttgcgggcaa aaccgttcgg gttacctcat ccactaaaat tgagcaagag cgcggccagg
     4981 cggcggttgg cgctgaagtt gaagtcgaag ccgtccccca atcagacggt tcttttctgg
     5041 cttcagaagt caaagtcatt cgcggcagcg gcggtggcag cgggtcggaa gtcaaatttt
     5101 atggcacgat cagtagcctt cctgcatcca gtgacctgcg tggcaactgg gtcgttgatg
     5161 gccgaaatgt catggtaacc gccagcacgt cgctggacca gtcacgtggt tcatttgcag
     5221 tcggtgtccg ggtcgaagtc gaaggctatg cccaatctga tgggtcagtc acggcttcat
     5281 ctatccacgt tgacgacaac agcacgggcg gcggctcaga agtcaaattt tatggcacga
     5341 tcaacagcct tcctgcatcc agtgacctgc gtggcaactg ggtcgttgat ggccgaaatg
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cacgtggttc atttgcagtc
     5521 ggtgtccggg tcgaagtcga aggctatgcc caatccgacg gttcagtgac tgccaaatcc
     5581 attcacgttg atgataacag caccggtggc ggtagcggtg gcatgtacat caagttttat
     5641 gatcggattc aaacccttcc agccagtgct gacctcattg ggacctggca gatcggagga
     5701 cgatcagttc aggtcaccag cagcacccag ttggatcgtt cccgtggcaa ttttgcggtg
     5761 ggtgtcctgg tcgaagtcga aggctcacaa caagccgatg gcaccgtgac cgctaaagaa
     5821 attcacgtcg aaagcagtgg cggcggcgga tcaagcagcg gttcgtattt tgaattttac
     5881 ggtgtgattg agactcagcc agcaggcacc ggagttgtcg gtgattggac catcgctggc
     5941 cgaaccgtac gcgtgacgtc ttcaaccagc attgaaaccc ggaacgggac attgcaagtt
     6001 ggagcactcg tcgaggtcaa aggcgtggtt gattcagccg gtgtaatcac ggcttcccgg
     6061 attgaagtca agtccagttc aagcacgggt ggtggatcaa cccagtacag ccagttttac
     6121 ggcaacatcg aggcccttcc gacgtcatct gaccagcttg gagaatggcg gatttcgggt
     6181 cgcaccgtta ccgtcacggc caatacctat attgatcgcg aacatggccc agccgttgtt
     6241 ggcgcctatg ttgaagttga aggacttgtt caggcagacg gatcatttac cgcaaccaag
     6301 atcgagatca aggaaaagac tggttccaac gggcgctcat cgtttcacgg cattgttgaa
     6361 accctgcccg atacgtcaaa tctggttgga gattggaagg tcagtgggcg gattgttcgg
     6421 gtgacaacct caaccaaagt ttcccagcgt ggtggctcaa tttcggttgg aactcgggtt
     6481 gaagtcaaag gctcggaaaa ttcagacggc tcaataactg ccaccaggat caaacgtgat
     6541 tcatagaaag cggtcagtag tcagtagttg ataagtcagt agtcagtagc tttcacgtgt
     6601 agattttttg attgcattgc cacaggacga aacagggtgt gaattggatt gagcatcccg
     6661 aaccgcaacg cttcgcggtg caaaaccaaa tacctacacg tgaaaggggc tactctctgg
     6721 tacctgcttt gcaggctaaa actggatttc cagcttgttc caaagaaatc catcatggga
     6781 aatttgttcc taaacgagtc aaataaaacc agatacactt gacagaattt ctcaattccc
     6841 aacacctgac tactgactac tgactactga ctactgacga ttcaccgata ttactcttca
     6901 gcgccgtgtt tgcggacgac ttccacggtg caatgggcac gggtggttac cgccgtcgag
     6961 acactgccga gcaaccagcg gtcaaaccgg ctcaggttgc gagagccaac aaacacacag
     7021 tcggccccga tttgttcggc ttcttccatc aacacctctt ttggtccgcc ttccataatc
     7081 actgaaattg tttggaggcc agcttcttcg agccgcctgc gcgccattgc ctgtaactta
     7141 ctggcaaagc ccatcttttc gtcataggcc acgccataca aatcaaatgg tccggcagag
     7201 gtcactaaat gaacttccgt tccagccggc cagcggcggg tggcaacggt ttcaaccgcg
     7261 atttcggatc cctttgaccc gtcaatgccg atcaccagtt tgactggatc ggtcacagcc
     7321 gctttgccag gtcgaacgac gtggactgag cagtgagcct gggtcacaac ctgggttgaa
     7381 acactgccaa ataccagtcg cccaacggct gagcgcccgt gagagccaat cacgatcaag
     7441 tcggcgttaa actcaaccgc tttttctaaa atttcacggc ccggagaacc aaaacgtcct
     7501 tcagccgtca cctcccatcg cgggaacaga gttttgaccc ggtccaatcc agtttgtgca
     7561 atccgcaggg cttcctcggt tgcatcctga atggcgctgg aaaaactggt ttcgaccatg
     7621 ccgatgctcg gcggggggac ggccagaccc atttctgaca ctgtcaaaac aaaggcttcg
     7681 gcctcttcgg gtaatcctgc gcggagcaag ccgtcaattg cggcgtcggc acattctgaa
     7741 ccatcatagg cgatcaataa tttcattcgg tccgtcatat cagttcctcc cttcactgat
     7801 gggaattgcc tgcggattgt gggcaacaag caagtttatt tgctgcctta atctgcaatt
     7861 gatattccat gattgggcgg attccgggag ttttgccaac tgttattcca accagtgcca
     7921 agtggtttat tttgctacga aatatctgat tttcagcaag ttagctacct gtcagaaaaa
     7981 cacttgattg cattggttcc atcaatgctc cgggagagtt tccaggaaaa ttccccagct
     8041 acacggcaat ggcatctcaa ttcaaacctg ggatggggat tattgcgcag ggtctgaagg
     8101 cttgggaggg agtaaccggt ccagcatcgg cttcacccgt gaatcataac aatttgggca
     8161 aatcccatgg ctgaaatcag tcccggtttg atcgtgaata tattcatcta tctgttgcca
     8221 gtaatcattg tcatcccgaa tgcgtttgca ataacagcaa atcggcaaca actctttcaa
     8281 ctggttgatc tggcgggtaa aaccaaggat tcgttcggca actttgagcc tcagacgcag
     8341 ggttttcgtg gccatggttt tgagcaaaaa gtcatctacg ccatcttcca ttgcctgata
     8401 atagtggtca tcgtcagtgt gaactgccgt gaggagaata aaataagtgt agtcattgtg
     8461 ccggaaagct cgaacttgtt ggcacaactc aacaccattt aacctgggca tttcccagtc
     8521 gctgaccaca atccggattg tgtcatctgc tttcagagcg gccagggctt cttcgccatc
     8581 gcaggtgacc atcacttcgt acccggcacg ttccagactt aactgaacca gggtccgggt
     8641 tacctcgtcg tcatcagcaa tcagaacttt catgaattca ccggattcct cagagtggga
     8701 gaccagaaac agagcgttcc agcctcgggt gaatcatagt tctaaaaccc aaaacctaaa
     8761 acctgtttcg accaacatgt gtcttcatca tcgtgattga attgcacctt tttcgtcgag
     8821 gaactttttg aagtaatttt gaagctttgg agaaagcaat cggtcgctgg atttcaaata
     8881 ctcaagcatc tgattgagtt tggtgcgcac tatggggcag tacaaactcc ggggatgttc
     8941 ttcaacgaaa ttgacggctt tttcaatacc agtttcgatt tgttcattcg tagcccagaa
     9001 tgtatccagt ccgacggcaa attcagccag gtcggggtca ctcaacaaag caagtgcttc
     9061 ctgttctttg ggcagggcca ggtcacgggt gttgcccgga ctttcggcat aggcattctc
     9121 tccgatacat aatacgatca catcggcacc ggcagcagcc ttgcgcaagc cttccgcaaa
     9181 tatccgttgt atttcgacaa c
//
LOCUS       DAOQDO010000010         9033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4092_length_9033_cov_3.352751, whole genome shotgun sequence.
ACCESSION   DAOQDO010000010 DAOQDO010000000
VERSION     DAOQDO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4092_length_9033_cov_3.352751"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="PKZ53_00445"
     CDS             complement(<1..2267)
                     /locus_tag="PKZ53_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNJ38929.1"
                     /translation="MKHSRWGVIFLCAIVWASGYLLREQVVPGDLVSAHGGKGVSSSD
                     AIWSFTEEATRNSTALDDVESQAVPRTVILNPTALGQVLSLAPMEFTDAARTQSVVVS
                     LPMPDGSFSRFRVEESPIMEPELAAKFPELKTYRGQGIDDPTATTRFDWTPQGLHAIM
                     LSASGTVFVRPVVRGEPLVYVTCDSHHDFEGQGARHCSVGTPDDPVNGKAIKALHNRI
                     ETVPMQFSSGTQLRTYRLAVGVTAEFTQQYGGGTVAGGLNAIVSSTNLITAIFQRETA
                     IRFVLVARQNELVFTDTNTDGYTNGNNNALLTENQAKLDQVIGSANYDIGHVYATISS
                     PFSGVAEIGSVCQSQTKGTGASSIGLDPSGPTVVGGVAHEYAHQFSAPHSYNGTTAGC
                     SQREATTAWEPGSGTTIMSYSGVCDAENLANDADNYFHVGSLGNILTYSTSASDCSAK
                     TPTGNNPPVIGSLSNAAIPARTPFTLTASATDQNGDALTYCWEQFDLGQQGPPNNDNG
                     NRPIFRSYPPTASPSRTFPSLKYILENNGEPPATININGQTFLSGESLATTTRVMNFR
                     VTVRDNKAAGGGTASAGLQVSVQGDRGPFTVTSPTAGASWTPGSQQQVTWNVANTNTA
                     PINTANVRITLSTDGGTTFSTVLAASVPNNGSATVTAPGNVVANARVRVEAIGNIFFN
                     LSPAFSVRSGCQPITVGPATLPVAAAFTDYTAQLTASGGTQPITFRVSQGNLPENVAL
                     SPNGQITGVPQTTGQA"
     gene            complement(3130..4734)
                     /locus_tag="PKZ53_00450"
     CDS             complement(3130..4734)
                     /locus_tag="PKZ53_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNJ38930.1"
                     /translation="MGKYHQKLPASGPAREALRQKGQFWTPAWVAEAMVSYVIDPENF
                     SLFDPAVGDGVFFQAAQKVAFERGQKITLFGTEIDPEVLPVARQKGVSETDLAGVQIR
                     DFVLDPPSRAFPAIVGNPPYIRHHRLTAEVKSRLRRLATETIGAPLDGRAGLHVYFLI
                     QALRHLSPRGRLAFIVPADICEGKFAPRLWDWVTRQYCLEAVMNFVPEASPFPGVDTN
                     ALVFFIRNEPPQDHFCWGMCLSAQTSDLKQWVESGFSVAGQCLKAETRCLDEALKTGF
                     SRPPQSKREGPVLGDFARVMRGIATGANEYFFLTRKQATELDLPDDFLVSAVGRTRDA
                     TGDEITQEHLQQLDRADVPTWLFSPDGRDCEQFPSSVKAYLKHGEQLGIHHRTLIATR
                     RPWYKMETRAVPPFLFAYLGRRSVRFIRNSAGVLPLTGFLCVYPFSTEAEFLAKFWKV
                     LQHPETIANLALVGKSYGSGAIKVEPRALEKLQLPVKAMEQAGLPIFWQTQPAHDVQS
                     QLRGTTKKVVQLSLFATEDEKLVMCS"
     gene            complement(4727..5662)
                     /locus_tag="PKZ53_00455"
     CDS             complement(4727..5662)
                     /locus_tag="PKZ53_00455"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AccI family restriction endonuclease"
                     /protein_id="HNJ38931.1"
                     /translation="MATEDIELELNGHSLLPWSDYLLNPRRLRGSDFLMRWSQGVWSE
                     ERVTQAVNQTGNYFALPYGPSGTAPDNDPRAFELYFERLEKAGLGKVKRPDLLIFPKV
                     EAPLISSVVTQLGGVAELPFISETDDGMRTLLSKALIAIECENSLWRAERMPDYRTPL
                     TPQRRLAGKPGLKKAAVLPTVIVKGEDLHPLQSWQDQTGVEIHIWHVFYDLAFGISFN
                     KVNDLIAQGLIEPTTQVFQAPGGATTRKTIYKIYYRYAYLLGQAEQEPELKPMHIEDS
                     NGHILPFVKFEGGRLALSQECLQVLDGVREGNANG"
     gene            complement(5812..7377)
                     /locus_tag="PKZ53_00460"
     CDS             complement(5812..7377)
                     /locus_tag="PKZ53_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNJ38932.1"
                     /translation="MPLFKTISAAVFGIDAYTVQVEIDLSPRLESSEKQPPVTMVGLP
                     DATVRESRERIRAAIQNCGYFFPFQRIIVNLAPADIRKEGSGFDLPIAVGILGAVGDI
                     KDRRIEESLLVGELSLDGRLRPVKGALSMALKARDRGIKYLFMPRENSHETAVVTGID
                     TYAVESLPEVVSLLNADELPPPLKIDVTHLIEETNGHHPDFRDIRGQFHTKRALEVAT
                     AGGHNILMIGPPGSGKTMLAKRIAGILPALSFEEALDVTRIHSVAGLTGAQGLVTTRP
                     FRAPHHTISDAGLIGGGSVPRPGEVSLSHNGVLFLDELPEFERNVLEVMRQPIEDGKV
                     TISRAAVSLTFPSRFMLAAAMNPCPCGFYNDPTRECSCSPVQIQRYIARISGPLLDRI
                     DIHVDVPAVKFQDLAGETPGEPSTPIRERVTAVRNRQLKRFAKERAGTERVFCNAQMP
                     PPMIRKFCRVSEESKKLLENAITRMGLSARAYDRILKVSRTIADMEDAADIAPYHVAE
                     AIQYRSLDRSYWM"
     gene            complement(7526..>9033)
                     /locus_tag="PKZ53_00465"
     CDS             complement(7526..>9033)
                     /locus_tag="PKZ53_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ38933.1"
                     /translation="EKLKTTAKHTLIFGVGGIVNRALGFLLLPLYLTEIRTREYGVLN
                     LMMIVLQMMNLVLALGLGNALMRSYYDFEESERPLITATAFFFLFTLCLVVLTPMFFL
                     APQWAAVVLQDRSGRFIPHFQLLFVIAFFQILKLIPDAVLRIKMESIRYTSTVTVAFL
                     LQLLTIIYFVKVEQIGIYGILIGTLVGSVGETLGLFYWMRYDLKFGFHWAELKRMLGF
                     GSPLIFGRLSAMSLQSIDRFLLQIFAASPNAGQREIGLYGMANNVSGAITMLVTTPFG
                     AVWPSMQVSVMKDPDAPEYYARILTYVLYIASVLALGAACVTGDIFRYFGSYVESASV
                     VPMLAMAAVLDAASQVLSVGINIKRKTFVNPMIYISAALINILLNLYFIPHYGMRGAA
                     FTTVVGFVVLCFLRGYVSHAYLPVPYEWKRLIHLFLISIGLYWASTFIVTGRTAVDFI
                     LHTLVALSLPLCLWLTGFHDEKEERKLREYWARFRRQPEIVDSERSSEVAK"
BASE COUNT         2370 a         2328 c         2204 g         2131 t
ORIGIN      
        1 gcctggccgg ttgtttgtgg tacaccggtg atttgtccgt tcggcgaaag cgcgacgttt
       61 tcgggtaagt tcccttgtga aacccggaag gtgatgggct gggttccgcc gctggctgtc
      121 aactgggcag tgtagtctgt aaacgccgcg gccacaggta aggtcgctgg cccgacggtg
      181 atgggttggc accctgagcg cactgaaaaa gcgggcgaaa ggttgaagaa aatgttgcca
      241 atcgcctcga ctcgaacccg ggcgttggca accacattgc caggcgccgt gacggtggct
      301 gaaccgttgt ttggaacact tgcggccaaa accgtcgaaa aggtggtacc gccatcggtt
      361 gaaagcgtga tacggacgtt ggcagtgttg atgggtgcgg tgttggtgtt ggcaacattc
      421 caggtcactt gttgttgcga accaggtgtc cagcttgctc cggctgtggg tgacgtgacc
      481 gtgaagggcc cccggtcacc ctgaactgaa acttgcagcc cagcggatgc cgtcccgcca
      541 ccagccgctt tattatcgcg cacagtgaca cgaaaattca tgacccgcgt cgtcgtcgcc
      601 agggattccc cgctcaggaa ggtttgtccg ttgatattga tcgtcgcagg aggctcgccg
      661 ttgttctcca aaatgtattt taacgacgga aaggtccgcg atggacttgc ggtcggcgga
      721 tacgacctga aaatcgggcg attcccattg tcattgtttg gcggtccttg ctggccgaga
      781 tcgaattgtt cccagcagta tgtcaaagca tcaccgttct ggtccgtggc tgatgcggtg
      841 agcgtaaacg gggttcgcgc cggaatcgcc gcattcgata aggagccaat cacgggtggg
      901 ttgttgccgg tcggcgtttt tgccgaacag tcagaagcac tggtcgagta ggtcaagata
      961 tttcccaaac tgccaacgtg gaaatagttg tccgcatcat tggccagatt ttcagcatca
     1021 cagacaccgc tataactcat gatcgtcgtg ccactccccg gttcccaggc cgtcgtcgcc
     1081 tcgcgctggc tgcaaccagc cgtcgtcccg ttgtaggaat gaggggcact gaactgatgg
     1141 gcgtactcgt gcgccacacc gccgaccacc gtggggcctg atgggtccag accgattgac
     1201 gaagcacccg tgcccttcgt ctggctctgg cacaccgaac caatctccgc gacgcctgaa
     1261 aacggagatg aaatcgtggc atacacatgg ccaatatcat aattggcgct cccaatcacc
     1321 tgatccagtt tggcctgatt ctcggttaag agagcattgt tgttgccatt ggtatagcca
     1381 tcggtgtttg tatcggtgaa aacgagttcg ttttgcctgg cgaccagaac aaaccgaatg
     1441 gccgtttcgc gttggaaaat cgcggtaatg agattggtcg aagatacaat ggcattcaac
     1501 ccgcctgcga ctgtcccacc tccatattgt tgggtaaatt cagccgtgac gccgactgcc
     1561 aggcgatagg ttcgcaattg tgttcctgag gaaaattgca ttggaacagt ctcgatacgg
     1621 ttgtgcagag cctttatcgc ttttccgttc accgggtcgt caggggttcc gacggagcaa
     1681 tgtcgtgccc cctgaccttc aaaatcatgg tgagagtcac aggtaacgta aacgagaggt
     1741 tcacctcgga caactggtct aacaaatact gttccactcg ccgataacat aatcgcgtgg
     1801 agcccttgtg gtgtccaatc aaaacgggtt gtggcggttg ggtcatcaat cccttgtccc
     1861 cggtaggttt ttaactctgg aaactttgcg gccagttcgg gttccataat tggggactct
     1921 tcaacacgga aacgtgaaaa ggagccatcc ggcatcggaa gcgaaacaac gacagattga
     1981 gttctggcgg cgtcggtaaa ttccatcggc gccagactga gaacctggcc aagcgctgtc
     2041 ggatttaaaa taaccgttcg aggcacagcc tgggattcaa cgtcgtccag tgcagttgaa
     2101 ttccgtgttg cctcttccgt aaaagaccaa atggcatcgc tggaagatac ccccttgcca
     2161 ccatgagctg ataccagatc gcccggtacc acttgctccc gaagtaaata cccgcttgcc
     2221 caaacaatcg cacacaagaa gatcactccc catcgactat gtttcatcgg cctgaaattc
     2281 ataagggttt ctcctgattg aggataaaat taaaaactgg attgaaatta cctgaagaga
     2341 aaagggtgaa cccataacaa gtttttggcg gttacgttca tttttggcgt atcttcggct
     2401 catcagttca gccagagccg gcagccaaac ccaatagctc gccatgcctg gcaggttcaa
     2461 tgcaaaacct ggaagaccaa tcttttgcag accatcaata cctgggctga gcctatcggg
     2521 ttcaaaaaat ataaagtttt ttcagtcatt gaaactgttt gtcagacgat aatttcattg
     2581 tgaaagggga taaccagaaa tgattctgaa cctggtatcg ccagatatgg cacactggaa
     2641 ctaacccgcc gattgaacct caagaggcac aatcgttttc atgccaattc agtcagtgac
     2701 agtcgaagcc gaaaaaaatt aataaactgg attcttgtgg tgtagggaat gaatgtaagc
     2761 caatgaatgt tgaagggtga ggagcacgtg gtattgagaa acctataccg tcaggaaaga
     2821 gattgtgcaa gaaaattcct ccttctgtac aagatgaagt gccttcctgc ttctaccgga
     2881 ttttgtgctg gaggtaaatc agaagaaccg atacgtcagt accgtcagcg tcagtgggtg
     2941 gggctgcgtg tgccaaaccc cacccgctca cgcaggtagt tctgcgtaag ccctattatt
     3001 atacgaattt ggaatgaaat cctcttatta gaaaacagtc aagtgattca atcaaataaa
     3061 cttagtgaac tactgactac gggctactga ctacaaactg gtattaaatc tcgatttccc
     3121 agtttcaaat cagctacaca tcaccagttt ctcatcctca gtcgcaaata aagacagttg
     3181 aacaactttt ttggtggtac cccggagctg agactgaaca tcgtgtgccg gttgtgtttg
     3241 ccaaaaaatt ggtaatccag cttgctccat tgctttgacg ggaagttgaa gcttttccaa
     3301 cgcacgtggt tcgactttga tagcgcctga gccatacgat tttccaacca gagctaaatt
     3361 cgcaatcgtt tcgggatgtt ggagaacttt ccaaaatttc gccagaaact cagcctcagt
     3421 ggaaaacgga tatacacaca aaaatccagt caatggaagg actccggctg agtttcggat
     3481 aaacctcacg cttcgccttc ctaaataagc aaacaaaaac ggaggaacag cccgtgtctc
     3541 cattttgtac cacgggcgac gggtggcaat cagtgttcga tgatgaattc ccagttgttc
     3601 accatgcttc aaataggctt tgaccgaaga cggaaactgt tcacagtcac gcccatccgg
     3661 agaaaacaac cacgtcggaa catcagctct gtcaagctgt tgcaagtgct cctgtgtaat
     3721 ctcatcgcct gtggcatcac gggtacgccc aacggcagaa acaagaaagt cgtccggcag
     3781 atcaagttcg gtggcttgct ttcgggtcag gaaaaaatat tcattggcgc cagtggcaat
     3841 accacgcatc acccgagcga aatcacccag gaccgggcct tcccttttag attgcggtgg
     3901 tcgtgaaaac ccagtcttga gagcttcatc cagacaacgg gtttcagctt ttaagcattg
     3961 accagcaact gaaaaaccgg attcaaccca ttgcttcaaa tcactggttt gagctgataa
     4021 acacattccc cagcaaaaat gatcctgagg aggctcattt cggataaaaa agaccagggc
     4081 attggtatca accccaggaa aaggtgatgc ttctggaaca aaattcatca cggcttccag
     4141 gcagtattga cgagtaaccc aatcccacaa cctgggagca aactttcctt cgcaaatatc
     4201 agccgggaca ataaaagcca gtcgtccacg tggagacaaa tgccggagcg cctggatcag
     4261 aaaatagaca tggagcccag ctcggccatc aagcggagcc ccgattgtct cagtcgccaa
     4321 ccgccgcaac ctggatttca cctcagcagt caaccgatga tgtcggatat aaggcggatt
     4381 tccaacaatg gcaggaaatg ccctggatgg gggatccaag acaaaatccc tgatttgaac
     4441 tccagctaaa tcagtttctg aaacaccttt ttgccgcgcc actggcaaaa cctctggatc
     4501 aatttcagta ccgaagagag tgattttttg gccacgttcg aaagcaactt tctgtgctgc
     4561 ttgaaagaac accccatccc caacggcggg atcaaagaga gaaaagtttt ctgggtcaat
     4621 cacatagctg accattgctt cggcaaccca ggctggtgtc caaaactgtc ctttttgcct
     4681 cagggcttca cgtgcaggcc cggaagctgg tagtttttga tgatacttac ccatttgcgt
     4741 ttccctctct tacaccatca agaacctgca agcattcctg gctcaatgcc aaacgacctc
     4801 cttcaaattt cacaaagggc aaaatatgtc catttgaatc ttcaatgtgc attggtttta
     4861 attcaggctc ttgttctgcc tggccaagaa gataagcata gcgatagtag attttataga
     4921 ttgtcttcct cgtcgttgcc ccgcctggtg cttgaaaaac ctgggttgta ggttcaatta
     4981 gcccttgggc aataagatcg ttgactttat taaaactaat cccaaatgcc aaatcataga
     5041 aaacatgcca gatatggatt tctacccctg tttgatcctg ccaactttgc agtggatgaa
     5101 gatcttcacc cttgacaatt acagttggta aaacagccgc tttctttaat cctggtttac
     5161 cagccaggcg acgttgaggg gttaaggggg tacgataatc aggcattctt tctgcacgcc
     5221 aaagactatt ctcacattcg atagcaatga gagcttttga gagaagtgtt ctcatcccat
     5281 catcggtctc ggaaataaaa gggagttcag caacacctcc aagttgagtg acaactgagc
     5341 ttatcaacgg cgcttctact tttggaaaaa tcaaaagatc tggtcgtttg actttcccaa
     5401 gaccagcttt ctccaatcgt tcaaagtaaa gctcaaatgc ccgtgggtca ttatcaggtg
     5461 cggttccact ggggccataa ggtaaagcaa aataattccc cgtttgattt acagcttgtg
     5521 tcactcgctc ttcactccaa actccttgtg accaacgcat caaaaaatca cttccacgaa
     5581 ggcgacgtgg attcaaaagg taatctgacc acggaagcaa agagtgtcca ttcaactcta
     5641 attcaatatc ttccgtagcc acacatagtt ccaattcaaa acaatgcatg tttaacctct
     5701 ttgttctttt acagttgata agtcttcata gatcttaatc cactaagcca acctgatctt
     5761 atgtcgctac actacctttc atctatactt tatagcaatc agcaagtcga atcacatcca
     5821 gtaacttcga tccagactgc ggtattgaat cgcctccgcg acgtgatatg gcgcgatgtc
     5881 ggcggcatct tccatgtcgg caatcgtgcg gctgactttg agaatccggt cataagcccg
     5941 cgccgaaagc cccatgcggg taatggcgtt ttcgagcagt tttttgcttt cttcagagac
     6001 ccgacagaac ttgcgaatca ttggcggcgg catctgggca ttgcagaaaa cccgctccgt
     6061 tccagcgcgt tctttggcaa atcgcttgag ctgacgatta cgaactgccg tgactcgctc
     6121 gcgaatcgga gttgatggtt cacccggcgt ttcaccagcc agatcctgga atttcacggc
     6181 tggcacatcc acgtggatgt caatccggtc aagcaatggc cctgaaatgc gggcaatgta
     6241 gcgttgaatc tgcactgggg aacaactgca ctcgcgggtc gggtcattgt aaaacccaca
     6301 gggacagggg ttcatggccg ctgccagcat aaagcgcgac ggaaaggtca acgacacggc
     6361 ggctcgcgaa atcgtgactt tgccgtcttc aatcggctga cgcatcactt ccagtacgtt
     6421 gcgttcaaat tcagggagtt cgtccagaaa cagaaccccg ttgtgcgaca ggctgacttc
     6481 tccgggacgt ggaacgctgc cgccgccaat caatccagca tcgctgatcg tgtggtgcgg
     6541 tgcgcgaaag ggtcgggtcg tcaccaaacc ttgcgctccg gttagcccgg cgacgctgtg
     6601 aatgcgcgtg acgtcgaggg cttcttcaaa cgaaagcgcc ggtaaaatac ccgcaatccg
     6661 cttggccagc atggttttac cagaccccgg aggcccgatc atcaaaatat tgtggcctcc
     6721 cgctgtcgca acttcaagcg cccgtttggt gtgaaactgg ccgcgaatat cccgaaaatc
     6781 cggatgatgg ccgttggttt cttcgatcag gtgggtgaca tcaattttga gcggtggtgg
     6841 aagttcatct gcgttgagca gcgacaccac ttcaggaagt gactccacgg cataggtatc
     6901 aattccagtc acgaccgctg tttcatgact gttttcgcgc ggcataaaga ggtacttgat
     6961 cccccggtca cgtgctttga gcgccattga aagcgctcct ttgaccgggc gcagccggcc
     7021 atcaagcgat agttcgccaa ccaggagtga ttcctcaatt cgccgatctt taatatcacc
     7081 gaccgcacct aaaatcccaa ccgcaatcgg aagatcaaac ccggaacctt ccttgcgaat
     7141 atcggctggg gctaaattga cgataatccg ttgaaatgga aagaaatatc cgcaattttg
     7201 aatcgcggcc cgaatccgct cgcgactttc acgaaccgtg gcatccggta acccgaccat
     7261 ggtcaccggt ggctgttttt cagaggattc caggcgcggt gataaatcaa tttcgacctg
     7321 cactgtgtag gcgtcaattc caaacacagc cgcactgatt gtcttaaata atggcattcc
     7381 gacctccatc acctgtttga aagcattcag accaatgcaa ttaaagttaa attcaacatt
     7441 gaattgagtt tttcaatgtt tgactcgtct gaagcggctg tgtgcacgtg gaatgccaga
     7501 gtgacagggt gacggggtga ctttgctact tcgctacttc gctacttcgc tcagaatcta
     7561 caatttcggg ttggcgtcgg aaacgagccc aatattcacg gagttttcgc tcttcttttt
     7621 catcgtgaaa gccggtcagc cacaggcaaa gcggcaaact gagcgccacc agggtatgta
     7681 agatgaaatc aacggctgtt cggccagtca caatgaaggt actggcccag tacaaaccaa
     7741 tgctgatcag aaacaggtga atcaggcgtt tccattcata gggcactggc aaataggcgt
     7801 gtgagacata gccgcgcaaa aagcacagca cgacaaaacc aaccacggtg gtaaaggcag
     7861 caccacgcat tccatagtgg gggataaaat acaaattcag cagaatattg atcaatgcgg
     7921 ctgaaatgta aatcattgga ttcacaaatg ttttacgctt gatattgatg ccaacactga
     7981 gcacctgact ggccgcatcc agcacagcag ccatggccag catagggacg actgaggctg
     8041 attcgacata ggagccaaaa taccggaaga tatcaccagt cacacaggct gcgcccagtg
     8101 ccagaacgct cgcaatgtag agcacatagg tcagaatccg ggcatagtac tctggtgcat
     8161 ccggatcttt catcaccgaa acctgcatcg aaggccacac tgccccaaat ggcgtcgtga
     8221 ccagcatcgt gatcgcgccg gaaacattgt tggccatccc atagagaccg atttcacgct
     8281 gcccagcatt aggactcgcg gcaaaaatct ggagcaaaaa tcggtcaatt gattgcagac
     8341 tcattgccga taaccgcccg aaaatcaacg gactgccaaa tccaagcatt cgtttgagtt
     8401 ctgcccagtg gaatccaaat ttcaagtcat accgcatcca gtaaaacaac cccagggttt
     8461 ccccaaccga accgaccagc gtaccgatca agatgccgta aatgccgatt tgttcaactt
     8521 tgacgaagta aatgattgtc aggagttgca gcaaaaacgc gaccgtgacg gttgaggtat
     8581 agcggattga ctccatttta atccggagca ccgcatctgg aattagcttc agaatctgga
     8641 agaacgcaat cacaaataac agttgaaaat gcgggataaa gcgtcctgac cgatcttgca
     8701 gcaccactgc tgcccactgc ggcgccagga aaaacatcgg cgtcagcacc accagacaca
     8761 gagtaaaaag aaaaaagaag gccgtcgcgg tgatgagcgg acgctcgctt tcttcaaaat
     8821 cgtagtagga acgcatcaac gcattcccca acccaagcgc cagcaccaga ttcatcatct
     8881 gaagcacaat catcatcaaa ttcaacacgc catattcgcg ggtgcggatt tcagtcaaat
     8941 agagcggcaa cagcaaaaag cccagcgccc gattgacgat gcctccaaca ccaaaaatca
     9001 aagtgtgttt ggcggttgtt tttaacttct cgt
//
LOCUS       DAOQDO010000011         8791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4267_length_8791_cov_3.249657, whole genome shotgun sequence.
ACCESSION   DAOQDO010000011 DAOQDO010000000
VERSION     DAOQDO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_8791_cov_3.249657"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKZ53_00470"
     CDS             <1..912
                     /locus_tag="PKZ53_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38934.1"
                     /translation="GVRALGVLRADSQAPALIGALDSDPNLRIDIFRVFIKLGRTEYG
                     EYAIPYFSSDDREIRAQAIATAGLLRTHKAVESLNSVYRLGEDKRGFKANLNDFFKQV
                     PERQRITLEALSQIGDPASKDIFEGNLRRDEDRRQWANEGLARMGDTSFVETISRNRL
                     KEGSGRVKLAQAFALYKLGRPEYLDAIIKELEAITLGDQATGYLYEVLSPADLHGPVR
                     VATTGATVKILKVLGVIGTSESIPVVEPLLRDTNARVVNAANLAIQRIKLREQYQQGQ
                     LPVKPPLPQPQSPSRPRRTNPKDGGQS"
     gene            988..2625
                     /gene="murJ"
                     /locus_tag="PKZ53_00475"
     CDS             988..2625
                     /gene="murJ"
                     /locus_tag="PKZ53_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNJ38935.1"
                     /translation="MRSPSPDLASTSDSASKTTPRSHNPSASAFLVAAGILLSRISGF
                     AREWFFARYFGNSDVAGIFRAAQSIPNFLQNLLGEGVLSASFIPVYSRLLAEGNEADA
                     RKVAGAVASLLGAVVSVLVLIGIGLAPVLTKLIVPGFTGPHYDLAVQLVRIVFPGTGI
                     LVMSAWCLGVLNSHRQFFLSYSVQVVMNATVIVTLVVFRHQPGEILVVYTTWGATLGS
                     FLIFLVQLPNVLGLLKRFEPSFDVRFAPVWKVIQNFLPVVAGRGVVQISAYVDRILAS
                     FISPTALAVLANAQIVYMLPVSLFGMSFSAAELPEMSSTLGTSDEIAEQIRQRLIATL
                     RHVAFYVIPSAVAFWVLGDVVVGALFQNGRFQRGDTVLVWCTLAGLTIGLLAATMGRL
                     YSSTFYALHETRVPLKCSIARVGITAVLGYGLALEVPRLLGLKPFWGIVGLTISSGVA
                     SWVEYLLLRWILRRRIGSFRLPWRFGLTLWAIAGSAAVLGIAIKSVLMWLPPFVLAGV
                     VLGIYGVGYLAGTVWLGIPEADFLRTKLLGRLKRFLR"
     gene            complement(2691..5798)
                     /locus_tag="PKZ53_00480"
     CDS             complement(2691..5798)
                     /locus_tag="PKZ53_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ38936.1"
                     /translation="MKRFLTLLSLVLFAMTVVAVGTGKMQSGAVYQAPPAAQTAVSQL
                     EAVQPQLLSSEYVSLSDQHKVMISAEDSALFDELMATGSVKQVLDYGSFRVVTVGTDT
                     VSSLKGRAEIRDDMNVITLNGFHLDTTNPEATLATLPSAFSRQADVTKGVQTGAQAGL
                     YLVQFTGPIQDEWYAKLEKTGVHIVTYTSNNAYIVQASASQVQSLEKWKTNHSETVQY
                     VGFYEPAFRVSPTLRDRYQRNASALTDVTIQVVDGPRATETLNALKQISTQVLRQENK
                     VLEYRNITLTVPTDRIADISRMDGVFAVAYYTSPVLMDERQGQILAGNISGNAVGAPG
                     YLAWLQARGFSGTGQFDFTVDVTDDGVDRGSNTDVNTEFKVNGQSAGASRLVYNNNYT
                     GDSQADGGGGHGNVNASIIFGYNNLTGTAAEDAGGHNYGLGICPWVKVGNTKVFSNGS
                     GAQFTQEATVRLRAAYNGGARISNNSWGYRFANDYNADSQEHDVLVRDADNVASGNQE
                     MTVVFAAGNSGSSSFTVSPPGTAKNIICVAAFENDRQTGTDGCGTDNTGANNVNDVIG
                     FSGRGPASDGRIKPDIGGPGTHIQGAASRSTNYDGAGVCNQYWPTGQTLYAWSSGTSH
                     SAPAICGVAALIRQYFINQSWTVPSPAMVKAYMANATRYMTGVGANDTLPSNSQGMGS
                     AHLATCFDGVQRTMVDQTEVFGATGQTRTVSGTIVDNSKPFRVTLVWTDAAGSTTGNA
                     WVNNLDLEVTAGGQTYKGNVFSGRNSITGGTADTKNNMESVFLPAGVSGAFSVTVKAT
                     NIAGDGVRNNGDTTDQDYALVVYNGTTVAPTPDFTISANPTASSAITQGGSATTTVTV
                     APTNGYTGTVNLSVTGLPANTTANFVPTSITTSGTSTLTINTTASTPTGIFSLTITGT
                     DGALTHTATYTLTVGAVPVGDFSLTTTPKTGTWRRNSSYRVTVNVTRTGGHNAAITLS
                     QTASKSGLFSSVTFSPNPIAGTGKSSSATFRVAATAPTGAAVITITGNDGVRTRTATV
                     NIKVQ"
     gene            6711..8729
                     /locus_tag="PKZ53_00485"
     CDS             6711..8729
                     /locus_tag="PKZ53_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ38937.1"
                     /translation="MSILSRITTLVTVTLIILEIGVCQATSRFSPFQHSSTSRSRDVD
                     IILEKHIAAIGGHTAWNSIQSLERYSEIEVFGNIQKVYRLEDRKNHRRFARIEGAQGT
                     QENGFDGKRSWRRAPFFKGYLEDSDPQAVADRQPQPELYEYREKNLSFTQQPDETLDG
                     VKCLVLTTSEKDPLGHDIPIKYYFDAATYLLKQIVSGDAIKNTEQFDDYRTVDGRVLA
                     FSVKSINPHVTIKTKITQVKVNVPVDPKLFELGEVSSTGPSPATTPAVTPKAALPSIA
                     PGAKTLPENVRLETFELVWKTINESYWDATFGGVDWKSIHDQYLPKVKATEGNKEFHT
                     LLNQMVGELKRSHLSVVEPGQVQGLQTRSTQPAVGNIGLDLRWMKNQLVVKEVKPDSP
                     AAKQGIRPGYVVSKIDNKTPDTFLKEFKAERGGFALREEVERVRAANKFLQGEAGTKV
                     KLEVLDEHDQPHTFEVERQAQPAGMALSFEHRKLTPQIGYIRFNLFFGDLLDQFQKAL
                     AELQTTSGLIIDLRGNRGGAAQLTNALASVLSATDGTLGTMTFRYQSQPAAFQGTGKS
                     AYQGRVVILVDELTGSAAEVFSAGMQDMGRATVFGQTTAGAVLPALNQLLPTGGSLMY
                     VISNYTSPKGLVLEGRGTVPQVKAELTRQSLLAGQDNVLQMAIDSLKE"
BASE COUNT         1970 a         2221 c         2441 g         2159 t
ORIGIN      
        1 ggtgtccggg cattgggggt ccttcgggct gattcacagg cgccagcctt gatcggtgcg
       61 cttgattcag acccgaattt gcgaattgat atctttcgag tgttcatcaa actggggcgg
      121 actgaatatg gtgaatatgc catcccgtac ttttctagtg acgaccggga aatccgggcc
      181 caggccattg ccacggcggg gctgttgcga acccacaaag ccgttgagtc gctcaatagt
      241 gtctatcggt taggtgaaga taagcgcggg ttcaaagcca acctcaacga cttttttaaa
      301 caggtgccgg aacgccagcg cattacgctt gaagctcttt ctcaaatcgg tgacccggcc
      361 agcaaggaca tttttgaagg aaatctgcgt cgcgacgaag atcggcgcca gtgggctaat
      421 gaaggtctgg cacgaatggg agataccagt tttgtggaaa ccatttcgcg caatcgtctc
      481 aaagagggca gcggcagggt caaactggcc caggcgtttg ccctctacaa acttggacga
      541 cctgagtacc tggatgccat catcaaggaa cttgaagcca tcacccttgg cgatcaggca
      601 accgggtatt tgtatgaagt cctcagtcct gcagatttgc acgggccggt gcgggtcgcc
      661 acgactggcg ccaccgtaaa aattctcaaa gtgctcggtg tcattggcac cagcgaaagt
      721 atcccggttg tggagccgtt attgcgcgac accaatgccc gggttgtgaa tgccgccaac
      781 ctggccatcc agcgcatcaa gctccgcgag caatatcagc agggccagtt gccggtaaaa
      841 ccaccgcttc cacaacctca atctccatcc cgtccacgac gtaccaaccc gaaagatggt
      901 ggccagtcat aaaagagcga gtcgtgagga gcgagttgct cctcacgact cgctttcgtg
      961 tgctccttcg cgccttcacc cctccttatg cgttctccat ccccagatct tgcttcaact
     1021 tctgattcag cttcaaaaac aaccccacga tcacacaatc cttcagcttc ggcgtttctg
     1081 gtggcggctg ggattttact cagtcgcatc tctggatttg cccgtgagtg gttttttgcc
     1141 cggtattttg gcaactccga cgtggccggg atctttcgag cggctcaatc cattcctaat
     1201 tttttgcaaa atttgcttgg tgaaggcgtg ctttcagcgt cattcatccc ggtctactct
     1261 cgcctgctgg ccgaaggaaa cgaagccgac gcccgtaaag tcgctggggc ggtggcatca
     1321 ttgctgggtg cggtcgtgtc agttctggtg ctcattggaa ttgggctggc gccagttttg
     1381 acgaaattga tcgtgcctgg ttttactggc ccgcactatg acctggcggt ccaactggtg
     1441 cggattgtgt ttcctgggac gggcattctg gtgatgtcag cctggtgtct gggcgtgctc
     1501 aacagtcacc ggcaattttt cctgtcatat agtgtccagg tagtgatgaa tgccaccgtg
     1561 attgtgacgc tggtggtgtt tcggcaccag ccgggtgaaa tcctggtggt ctataccacc
     1621 tggggcgcca cccttggcag tttcctgatt tttctggtcc agctccccaa cgtactgggg
     1681 ttgctcaaac ggtttgagcc gtcttttgat gtgcgctttg cgccggtctg gaaggtgatt
     1741 cagaattttt tgccagtcgt tgccggacga ggtgtggttc aaatcagtgc ctatgtggac
     1801 cggattctgg ccagttttat ttcacccacg gccctggcgg ttctggccaa tgcccagatt
     1861 gtctatatgt tgccggtgag tttgtttggc atgtcatttt cagccgctga actccctgaa
     1921 atgtcgagta cactcggaac cagcgatgaa attgccgagc aaatccgtca acggttgatt
     1981 gcaacgctac gccacgtggc attttacgtc attccgtcag ccgtcgcctt ttgggttctg
     2041 ggcgatgtgg tggtgggcgc gttgtttcag aatgggcgct tccaacgcgg tgataccgtg
     2101 ctggtctggt gtacgctcgc cggactgact atcgggttgc tggcggcgac gatgggacgg
     2161 ctttattcct ccacatttta tgctctccac gagaccaggg ttccgttgaa atgctcgatt
     2221 gcccgggtgg gcataacggc tgttcttggc tatgggctgg cactcgaagt tccacgtctg
     2281 cttggattaa agccgttttg gggaattgtt ggattgacga tttcttcagg cgttgccagt
     2341 tgggtcgaat acctgttgtt gcgatggatt cttcggcgcc ggattgggtc gtttcgcctg
     2401 ccctggcgat ttggactcac gttatgggcg attgctggga gcgcggcggt gctggggatt
     2461 gcgatcaaat cagtgttgat gtggctccca ccctttgtgc ttgccggagt ggttttggga
     2521 atttacggag tggggtatct ggcgggaacg gtctggctgg ggatacctga ggctgacttt
     2581 ctgcggacca aactgctggg acggctcaaa cggtttttga ggtagtgaag tcgtaagaaa
     2641 aagcgagtcg caagtcgtga tcgtcacgac ttgcgactcg ctttggttca ttactgaacc
     2701 ttaatgttca ccgtggccgt gcgggtgcgc acgccatcgt ttccggtaat ggtaataact
     2761 gctgctccgg ttggggcggt tgccgcaact cggaacgtgg ctgagcttga ttttccagtc
     2821 ccggcgattg gatttgggct gaaggttaca ctgctaaaca accctgattt actggccgtc
     2881 tgagacaggg taatcgcggc attatgacca cccgtacggg tgacattgac cgtaacgcga
     2941 taggatgaat tccgccgcca ggtgccagtc ttgggggtgg tggtcaggct gaaatcaccg
     3001 actggtaccg ccccaacggt cagcgtatag gtggccgtgt gggtgagcgc accatctgtc
     3061 ccggtgatcg tcagtgagaa gatccccgtt ggcgttgacg ctgtggtgtt aatcgtcagc
     3121 gttgacgtgc cagacgtggt gattgaggtt ggtacgaagt tggccgtggt gttggctggt
     3181 aatccggtaa ccgacaggtt gaccgttcct gtgtacccgt tggtcggggc aaccgtcacc
     3241 gtcgtggtgg ccgaaccgcc ctgggtgatt gcacttgatg ccgttggatt ggcgctgatg
     3301 gtaaagtcag gtgttggggc aaccgttgtg ccgttataga cgaccagggc ataatcttga
     3361 tcggttgtat cgccattgtt gcgtacgcca tcgcccgcaa tattggtggc cttgacagtg
     3421 acgctaaagg caccggaaac accagccggc aggaagaccg actccatgtt gtttttggtg
     3481 tcagccgtgc cacccgtaat tgagttgcgg cctgagaaca cattgccttt ataggtttgt
     3541 ccacctgctg tcacttccag atcaaggtta ttgacccagg cattgccagt tgtggaaccg
     3601 gcagcatcgg tccagaccag ggtgacccga aatggcttgc tattgtccac gatggttccg
     3661 ctcacggtcc gggtttggcc agtggcgcca aagacttcgg tttgatcaac catcgtccgc
     3721 tgaacgccgt caaagcaggt tgcgagatgc gcactgccca tgccctggct gttggaaggc
     3781 agagtgtcgt tggcgccgac tccggtcata tatcgggtgg cattggccat ataggctttg
     3841 accatcgccg ggctcggaac cgtccagctc tgattgatga agtactgccg aatcaacgcg
     3901 gccacaccgc agatggccgg cgccgagtgc gatgtacctg atgaccaggc atagagtgtc
     3961 tgccccgttg gccagtactg gttgcataca ccagcaccgt cataattggt tgagcgtgag
     4021 gccgcacctt gaatgtgcgt tcccgggcct ccgatatcag gcttgatacg tccgtctgag
     4081 gctggtccac ggcctgaaaa tccaatcaca tcattgacat tgttggcgcc ggtattgtct
     4141 gttccacacc catctgtgcc tgtttgccgg tcattttcaa atgcggcaac acagatgatg
     4201 tttttggcag tccctggagg cgacaccgta aacgaactgg agccactgtt tccggcggca
     4261 aacacaaccg tcatttcctg attgccgctg gcgacgttgt cagcatcacg gacgagcaca
     4321 tcatgttcct gtgaatcggc gttgtaatca ttggcaaacc gatatcccca gctattgttg
     4381 ctgatacgag caccaccatt ataggccgct cgcagtcgca ccgtggcttc ctgggtaaac
     4441 tgagcaccag agccattgct aaagaccttg gtgtttccaa cttttaccca cgggcaaata
     4501 cccagaccgt aattgtgccc accagcatct tcagcagcag ttccagtcaa attgttgtat
     4561 ccaaaaatga ttgaggcatt gacgttaccg tgtccgcctc caccatcagc ctgtgaatca
     4621 ccggtataat tgttgttgta taccaggcgg ctggcaccag ccgattgacc attgacctta
     4681 aactcggtat tcacatccgt gttgctaccc cggtcaaccc cgtcatctgt gacgtcaacc
     4741 gtaaagtcaa actgcccggt cccgctaaag ccgcgagctt gcaaccatgc cagatagcca
     4801 ggggcgccga ccgcattccc gctgatattt ccagccagaa tctgaccttg ccgttcatcc
     4861 atcagcactg gtgatgtgta ataagccacg gcaaaaaccc catccatccg gctgatatct
     4921 gcaatccggt cggtggggac ggtgagtgtg atgttgcgat attcgagaac tttattttcc
     4981 tgacgcagaa cctgagtgga aatctgtttg agggcattga gtgtttccgt tgcccgtggg
     5041 ccgtccacga cctgaattgt gacatcggtc aacgcgctgg cattgcgctg atacctgtcg
     5101 cgcagggtgg gcgagacccg gaaggctggc tcataaaagc cgacatactg cacggtttcg
     5161 ctgtggttcg ttttccattt ttcaagggat tggacctggc tggcgctggc ctggacaatg
     5221 taggcattgt tggaggtata ggtcacgatg tgaacgccgg tcttttccag tttggcatac
     5281 cactcatcct gaatcggccc ggtaaactgg accagataga gacctgcctg agcaccggtt
     5341 tgaacacctt tggtcacgtc tgcctgtcgg ctaaaggctg atggcagcgt ggccagcgtg
     5401 gcttctggat tggtggtgtc cagatgaaag ccattgaggg tgatgacgtt catatcatcc
     5461 cgaatttcag cccggccttt gaggctgctc acggtatcag tgccaaccgt gacgacgcga
     5521 aaactcccat agtccaggac ctgcttgacg ctgcccgtgg ccatgagttc atcaaacaaa
     5581 gcggaatctt cagccgaaat catgacctta tgctggtcgc tcaggctgac atactcactt
     5641 gaaagaagtt gcggttggac ggcttccagt tgtgaaaccg cagtttgggc tgctgggggg
     5701 gcttgataga ccgcgcccga ctgcattttc ccggtgccca cggccacaac ggtcatggca
     5761 aacagcacca ggctgagaag tgtaagaaaa cgcttcattc ggcaaactct cccttgaatt
     5821 gcttttcctg tgaggtgaaa aggaaatcga gaatacacaa aaacgatgat tggttagcga
     5881 ggccagaagc tggcacatac cgtttttgta gacgggttac aactgcaatt tcttccgtga
     5941 gataaaattt gtcctggcgg aagaataaga aatttgaggc acccagggaa cacatgtgct
     6001 ctgcgataac aagaagggtt cagacacctg aggtgatgaa agagcggctg attcctgcag
     6061 ttggaagccc tcttcggctg atatctgaca atgatgattg agtatggttg tcagtacgga
     6121 ctaccagacc ggcagaccga aaataaaaaa tgggcctgaa gcttaccacg gatcgaaggc
     6181 gggtcaaggc aggtctgggc gggttcaacc caaagtaagt gataacttac tttaggctga
     6241 atgatttaaa aaaattcaaa aaaatgaagg aggttgtccg gaaagcgctg atttgagtaa
     6301 cgagccaggg tcaattttgg gaagtcgcgg ttccaggcag tgccgataaa ttgtatttag
     6361 atccatacat ttgtgctgcc agctcaaaaa tagaaaaaat caaaaatagt actttgggaa
     6421 gaagccactg cttctggcat cggcaaatcc tcttttcctg atatggggag gggtttcctc
     6481 atttcaggaa ttgttcactg atcaaagtct attgcgaatt tatctggctt gaaatagcgc
     6541 tgtggcgata aatgctttat ttaaacatgt tatatggatt tcaatggatt tccgtagcta
     6601 aaaatagagg gggttgcgcc gtaacctgtg tctggtaccc attttgcact caggcatatc
     6661 tggaaccatc gcacatcata tcttcaagtt aaaaacggag ccttctctca atgagcatct
     6721 tgagccgaat tacgaccctt gtaaccgtta ccctgatcat tctggaaatc ggtgtctgtc
     6781 aggcaacctc tcgattttcc ccattccaac attcgtcaac gagccgttca cgcgatgttg
     6841 atataattct agaaaaacat attgccgcca ttggtggtca caccgcctgg aattcaattc
     6901 aatcgcttga gcgatacagt gaaatcgaag tctttggaaa tatccagaag gtctatcgcc
     6961 tcgaagaccg caaaaatcat cgccggtttg cccgaatcga aggtgcacaa gggacacagg
     7021 aaaacgggtt tgacggaaag cgatcctggc ggcgggcgcc gtttttcaaa ggctacctgg
     7081 aagattctga tccccaggcc gtggccgacc gtcaacccca gcctgaactg tatgaatatc
     7141 gggaaaagaa cctgagcttt acccagcaac cagatgaaac cctcgatggt gtgaagtgcc
     7201 tcgtcctgac caccagcgaa aaagacccgc tgggacacga cattcccatc aaatattatt
     7261 ttgatgctgc tacctacctg ctcaaacaaa tcgttagtgg tgatgcgatc aaaaataccg
     7321 agcaatttga tgattaccgg acggttgatg gtcgcgttct ggcgttttcg gtcaaatcca
     7381 tcaaccctca cgtaacaatc aaaaccaaaa tcacccaggt caaagtgaat gttccggttg
     7441 acccgaagct gtttgaattg ggtgaagttt cctcaactgg tccgtcacca gccaccacac
     7501 cggctgtgac accgaaagcc gcgcttccct caattgctcc aggtgcaaag accttgcctg
     7561 aaaacgtgcg gcttgagacg tttgaactgg tgtggaaaac gatcaacgaa tcgtactggg
     7621 acgccacgtt tggtggggtg gattggaaaa gcattcacga tcagtatttg ccgaaagtga
     7681 aagcaaccga aggcaacaaa gagtttcaca ccctgctcaa tcaaatggtt ggtgaactca
     7741 agcgctcgca tttaagcgtg gttgagccag gtcaggttca ggggcttcaa acccggtcaa
     7801 cccagccagc ggtcggaaat attgggcttg atttgcgctg gatgaaaaat caactggtgg
     7861 tcaaagaagt caaaccagat tcgcccgctg ccaaacaggg aatccgccct ggatacgtgg
     7921 tgtcaaaaat cgacaacaaa actccggaca cgttcctcaa agagtttaaa gctgagcgcg
     7981 gtgggtttgc gctccgtgaa gaagtcgagc gcgttcgagc ggccaacaaa ttcctgcaag
     8041 gtgaagccgg caccaaagtg aaacttgaag ttttggatga gcacgatcag ccgcatacct
     8101 ttgaggttga acgtcaggcc cagcctgcgg gcatggccct gagctttgag catcgcaagc
     8161 taacgccgca gattggatat attcggttta acctgttctt tggggatttg cttgaccagt
     8221 ttcagaaggc actcgctgaa ctccagacaa cctctgggtt gatcattgac ttgcgtggaa
     8281 atcgtggagg cgccgcccaa ttgaccaatg cgctggccag tgtgttgagt gccaccgatg
     8341 gcacgttggg aacgatgacc tttcgctatc aatcacaacc ggctgcgttt caaggtacgg
     8401 gaaaatctgc ctatcaggga agagttgtga tcctggtgga tgaactgacg ggaagcgcgg
     8461 ctgaagtttt ttccgccggg atgcaggata tggggcgggc aaccgttttt ggccaaacga
     8521 cagcgggcgc ggtgcttccg gcgttgaatc agcttctgcc aaccggcggc tcgttgatgt
     8581 atgtcatttc aaactacacc tcacccaagg gacttgtgct tgaaggtcgc ggaactgttc
     8641 cacaggtcaa ggccgaacta acccgtcagt ccttgctggc cggtcaggat aatgtgctcc
     8701 agatggccat tgattcactg aaagagtgaa aaaaccgggg ctgaagaagg cgggctgaag
     8761 aaggcgggct gaagaaggcg ggctgaagaa g
//
LOCUS       DAOQDO010000012         8694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4340_length_8694_cov_3.232782, whole genome shotgun sequence.
ACCESSION   DAOQDO010000012 DAOQDO010000000
VERSION     DAOQDO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_8694_cov_3.232782"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1666)
                     /locus_tag="PKZ53_00490"
     CDS             complement(47..1666)
                     /locus_tag="PKZ53_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38938.1"
                     /translation="MKTTRYLVALLLSLLFIDTGTQAQKPTSEYFESGRPRLIILVHG
                     ATDAPRYHPEKHIGTLQHARDYWEYTFVQNLIGATSDLGTLSGVRLNSETWTGAILRE
                     SEPLDHVVMADSALKAFNATRRLPEMSVLLTYRDGSETLASQGRTLIRQVYAHYARLT
                     QVSGVQPQIIWVGHSMGGNVIRYVLSNPIEAFNNVRLTAAERTQADFLRDRTVFAVTM
                     GTPHEGSPLADKFTQIAQFLGQNPSPLREFYILAGMNSPREHVIGWLGADSDSTRHLR
                     TDFWANQNIGALAPEAARRTDESLIPMYCLGSRSPGRTFFDDPNRDPVTLAGEVGGQD
                     TKTLYETIGLMSLDYALHATPGTPNHWGSVPPSSRDLDWVRRAFLTLLPPGELSEPRD
                     QPELFPLYYLKVPWRRETRGLFDLIPTFVPATANRPGADNSFSTRAADGEIDNDGMVG
                     IDSALGIKLGTTTLEYFDHSREWPMAGGLVRGSWYRLYSGPWNWDDHGSLRRNPETAR
                     WLRQHLFRSAGPWVGANSISQWDPEQLGSLE"
     gene            complement(1874..3280)
                     /locus_tag="PKZ53_00495"
     CDS             complement(1874..3280)
                     /locus_tag="PKZ53_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ38939.1"
                     /translation="MRIVSVLCCFFWLGAWSPFLAQSPLASNEIEKNYREAAAKIIGT
                     AMVDHGDFARLEYLTDRIGNRLSGSPQLDLAIQWAVAAMQADKLDRVQAEPAKVPHWV
                     RGNESCELLTPKKQKLTMLGLGNSVGTPREGITAEVVAVRDFEELKALGDAVKGKIVL
                     YSYPMRKDLAPDNAYGDAVKYRGQGPTAAAQQGAVAVLIRSITTRSLSTAHTGSTHYA
                     AEIPAIPAAALSPEDADMVTRLCAAGQKPTVTLMMDAHMLPEVESANVIGEIRGREKP
                     DEVVVISGHLDSWDVGSGASDDGAGCVMAMEAAHILKKLNLQPRRTIRVVLFTNEENG
                     LGGARAYAAAHKAEFEKHIAAIECDSGAGRTSGFSFTGKGRSLATVEQIAVLLKSIDA
                     QRIRHEDRTGADISVLTAAGVPSFGMDSDQTHYFDFHHTHADTLDKINPNDLAQNTAA
                     LAVLAFILADLPEPLARD"
     gene            complement(3387..3659)
                     /locus_tag="PKZ53_00500"
     CDS             complement(3387..3659)
                     /locus_tag="PKZ53_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38940.1"
                     /translation="MANHITSLVSLDFVAGKTGDIDASMNANIPFLNEAGVLENSQML
                     QFNELNEPLPADDNVLTTETTFTFDTSVLGGEEDGSSNSILRLQTV"
     gene            3704..4876
                     /locus_tag="PKZ53_00505"
     CDS             3704..4876
                     /locus_tag="PKZ53_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ38941.1"
                     /translation="MNTNELEKTTQQAAGSLRAAVTQASQGLIERESLVETIVLAAVA
                     GEHVLVIGPPGTAKSEAVRRVAQALGGQYFEYLLGRFTEPSEIFGPIDLQKLKSGVVE
                     IETTGMLPEAEIAFLDEIFLGSTAILNTLLNLLNERVFRRGHTRMAVPLRICVGASNR
                     LPEDESLAAFADRFLIHTFVEPIPDPRLEELLEGGWSLAGQSAFQSPGQPASVADLDL
                     LALAARKCEMGTVRGILAHAIRRLRQAGVLLSDRRVVKAQRLVAAAAVLAGRNQPAES
                     DVWPIVLTVPTLEQQAVARESLRDLLQHSQNQALSSLAEETSQGPLARATRIARAAEH
                     LLTIKPATPEALNEWKLKLEGIAREIDACFLPEKLPSELVEFRQKIVLEMMPKTSG"
     assembly_gap    5041..5140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6141..7706
                     /locus_tag="PKZ53_00510"
     CDS             6141..7706
                     /locus_tag="PKZ53_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38942.1"
                     /translation="MLFPEHPLDDETTNLDVIRPELNLEKWPALWQPIQSKNKQNKVV
                     IEREVMQLNNKFLARVVATANSEYGPLTTEDQKLYYALQKVWEDSGRQRRIVFSRRRI
                     ATILGKQWGSKVSKAINDSLFRLRGTLFVWTDSFYDSTNQKTIKTLGTFNILSDLKIA
                     EESVDGHRTTEECYCEFHELIYKNLLNGYTKPLLFTTVVSFEDGIAQMLYSHLDLMLA
                     NNDHYERRTRELFDDLQLQGNEYKFVSRRKRTLERILPTLQDKPTSRGGVLRLVLDKT
                     RGGKDFKLIVERVYLHNPEPVEDQAPISASQPAPPAPVITGDDPADSGETPEPAELIQ
                     ARLDLDQESDDLVRYFLKTFRINRKHPRPTELAEARELIYEYQLTREKSRWIVDFSLK
                     ASKETRFVPKSFNGIVQYTDRALEAWQLNLLEAENLPLPLVPPTPEPEDPILAAEKAE
                     IEESQRLETLGEELFSRLSEAERTTLVASELETLRKSEHWKVYARMEKNVLHEHISWL
                     VGRKLALQELGRL"
     gene            complement(7887..8318)
                     /gene="arfB"
                     /locus_tag="PKZ53_00515"
     CDS             complement(7887..8318)
                     /gene="arfB"
                     /locus_tag="PKZ53_00515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNJ38943.1"
                     /translation="MAEPLEINEFITIPTENLTWKAVRSSGPGGQHVNTTATCVELRF
                     DLAGCTVLGTGVKERLSALAGSRLVGGDTLLITSQLSRDQARNLDDAREKLRKLILRS
                     LEPPRPRRPTAPTRASQERRIAEKTRRSQTKRIRTGKPDFD"
BASE COUNT         2133 a         2250 c         2159 g         2052 t
ORIGIN      
        1 ttcttcattc ttcattcttc attcttcatt cttcattctt cattcttcat tcaagactgc
       61 ccagttgttc tggatcccac tgactgatgg aatttgctcc cacccagggg ccagccgacc
      121 tgaagagatg ttgacgtaac catcgggcag tttccgggtt gcgtcggagt gatccatggt
      181 catcccagtt ccacggacct gaatagagcc gataccacga cccacgcacc aaaccaccag
      241 ccatcggcca ttcacggcta tggtcaaagt attccaacgt ggtcgtgcca agtttgattc
      301 ccagggccga gtcaatgcca accataccgt cattatctat ttcgccatcg gcggcccggg
      361 ttgagaaact attgtcggca cctgggcgat tggccgttgc aggtacaaag gttggaatta
      421 agtcaaaaag tccccgtgtc tcacgccgcc acggcacttt gaggtaatag agcggaaaaa
      481 gctctggctg atcgcgaggt tcagagagtt cgccaggtgg caacagtgtc aaaaaggctc
      541 gtcgaaccca gtccagatcg cgtgaggatg gcggcacact gccccagtga tttggtgtcc
      601 caggcgtggc atgcagggca taatccaacg acatcaggcc aattgtttca tacaatgttt
      661 tggtgtcttg cccaccgact tcgccggcca gcgtgaccgg atcgcggttt ggatcatcaa
      721 aaaatgtgcg gcctggactt cgacttccca aacaatacat cggaatcaaa ctttcatcgg
      781 ttcggcgcgc tgcttctggt gccagagcac cgatattttg gttggcccaa aagtcagttc
      841 gcaaatgtcg ggtcgagtct gagtccgctc ccaaccagcc aatcacgtgt tcacgcgggg
      901 aattcatccc ggccagaata taaaattcgc ggagcggcga agggttttga ccaaggaatt
      961 gtgcaatttg ggtgaactta tcagccagtg gcgacccttc gtgcggcgtt cccatagtca
     1021 cggcaaagac tgttcgatcc cggagaaaat cagcctgggt tcgttctgcc gcggtcaacc
     1081 ggacgttgtt gaaggcttca atggggttac tcagtacata ccgaatcaca ttcccgccca
     1141 tactatggcc aacccagata atttgcggct gaacgccact gacctgggtc aggcgcgcat
     1201 agtgcgcata cacctggcgg atgagggtcc gtccttgact cgccagggtt tcagaaccat
     1261 cgcgatacgt cagcaacaca ctcatttccg gcaagcgacg tgtggcgttg aacgctttca
     1321 gggctgaatc tgccatcaca acatgatcga gcggctcaga ttcacgcaat atcgcacctg
     1381 tccaggtttc agaattgagt cgaacacctg acagcgtgcc caaatccgaa gtcgcaccaa
     1441 tcagattttg aacaaacgta tattcccaat aatcgcgggc gtgttggagc gttccaatgt
     1501 gtttttctgg atgatagcga ggagcatcgg ttgcaccgtg aaccagaata atcagtctgg
     1561 gccgccccga ttcaaagtat tcagaggttg gtttttgagc ctgggttccc gtatcaataa
     1621 aaagtaggga tagcagcaac gcaaccaaat agcgtgtggt tttcatacac acctcccgca
     1681 gtcaaaagat gaaatcacgc gacaccggag gcggaatttg agccaaaagc aatctgcaag
     1741 aagttgtctg caaagcccgt accacgattt tcttccgacc tgatcgggac ttgatcaaat
     1801 caaaatagtg aaaaattctg gttctttgcg caagattccc cagtggtagg gttgaacctg
     1861 gcgcttatgg acattagtcc cgagctaatg gttcaggtaa atcagccaga ataaaggcca
     1921 gtacggccag tgccgctgtg ttttgagcca aatcattggg attgatcttg tccaacgtgt
     1981 cagcatgggt gtggtgaaaa tcaaaataat gggtctggtc agaatccatt ccaaagctgg
     2041 gaactccggc agcagttaac accgaaatat cagccccagt ccgatcttca tggcgaatcc
     2101 gctgagcatc aatgcttttg agcagtacgg caatctgctc tacagtcgcc agactccggc
     2161 cttttccggt gaatgaaaat ccagatgtcc gcccggcgcc agaatcacat tcgatggcag
     2221 caatgtgctt ttcaaactct gctttatggg cagcagcata agctcgtgcc cctccaagtc
     2281 cgttttcttc attggtaaaa agcaccaccc gtatggttcg ccggggttgg agattcagtt
     2341 tcttcaaaat atgggcggct tccatagcca tcacgcatcc ggctccgtcg tcgctggctc
     2401 cgctaccgac atcccacgaa tcaaggtggc cgctaataac cacaacttca tctggttttt
     2461 cccgcccgcg aatttcccca atcacattgg ctgattcaac ctcgggcagc atatgggcgt
     2521 ccatcatcaa agtcacggtt ggtttttgcc cggcggcaca aagacgggtc accatatcag
     2581 catcttcagg tgacaaagct gccgctggaa tggctggaat ttcagcagca taatgagtgg
     2641 aaccagtgtg ggcagtgctg aggcttcggg ttgtgatgga acgaatcaaa acagcaactg
     2701 ccccttgctg tgccgcagcg gttggtccct ggccgcgata ttttacggca tcaccgtagg
     2761 cattgtccgg tgccaggtct tttcgcatgg ggtagctgta caacacgatt ttccctttga
     2821 cggcgtctcc gagagctttc agttcttcaa agtcacggac cgcgacaact tctgccgtga
     2881 tgccttcacg aggcgttccc accgagttcc ccaaccccag catggtcagt ttctgcttct
     2941 ttggggtgag gagctcacaa ctttcattac cacgcaccca atggggcact ttggcaggtt
     3001 cagcctggac ccgatccagc ttgtctgcct gcatggcggc aacggcccat tgaattgcca
     3061 ggtcaagctg tggactgccg ctcagtcggt tgccaatgcg gtcggttaaa tattccagcc
     3121 tggcaaaatc accgtggtca accatagcgg ttccaatgat tttggcggct gcttctcgat
     3181 agtttttttc tatttcgtta gatgccagtg ggctttgagc cagaaacggt gaccatgcac
     3241 caagccaaaa aaagcaacac aacacagaaa caattcgcat tgaagaaagt cctttaccgg
     3301 atgaaaggga atgttgaaag tggaaatggt gtctacacca agagctattc ccttttctac
     3361 cgtccttacc gaaaagaaat tcatttttac acagtttgta aacggagaat ggagttggaa
     3421 gacccatctt cctctccccc caaaacactt gtatcaaagg taaaagttgt ttccgttgtc
     3481 aatacattat catccgcagg caatggttca ttaagttcat tgaattgaag catttgacta
     3541 ttttctaaca cacccgcttc attcaaaaat gggatattgg cattcatact ggcatctatg
     3601 tcaccggttt ttcccgctac aaaatctagg gagaccaaag atgtgatatg gttggccaaa
     3661 ttgcctttct ctcaaggcta aaccctaacc ggtacaattg tgcatgaaca ccaacgaact
     3721 tgaaaaaaca acgcaacagg cagccgggtc gcttcgggcg gctgtcactc aggccagcca
     3781 gggtttgatc gaacgtgaat ctttggtgga aaccattgtt ctggcagccg tggctggcga
     3841 acatgtcctg gtcattggtc cgcctggaac cgcgaaaagt gaggctgtcc gtcgggttgc
     3901 ccaggcatta ggcggtcagt actttgaata cctgcttggg cgttttactg agcccagcga
     3961 aatttttggc cccattgacc tccaaaaact gaaatccggc gtggttgaaa tcgaaaccac
     4021 cggcatgctg cccgaagctg aaattgcatt tctggatgaa attttccttg ggtcaaccgc
     4081 catcttaaat acacttttaa atttgttgaa tgaacgagtc tttcgacgcg gacacacccg
     4141 gatggcggtg ccgctccgaa tttgtgtcgg cgcatccaac cggttgccgg aggatgagtc
     4201 gctggccgct ttcgctgacc ggtttttgat tcacacgttc gttgaaccaa tcccggatcc
     4261 gcgacttgaa gagctactcg aaggtggctg gtcactggcc ggccagtcag ccttccagtc
     4321 ccctggccag ccggcatcgg tggccgacct tgatttactg gcactggcag cccggaagtg
     4381 tgagatggga acggttcggg gaattctggc ccatgccatt cggcggcttc gacaggctgg
     4441 ggtgctcttg tctgatcgtc gggttgtcaa agcacagcgg ctggtggctg ccgctgccgt
     4501 gctcgctggt cgaaatcaac cagcggaaag cgatgtctgg ccaattgtgc tgaccgtacc
     4561 aacacttgaa caacaggccg tcgcccgcga atcgttgcgt gatctgctcc aacattctca
     4621 aaatcaggcg ctctcatcac tggctgaaga aacttcacaa gggccactgg ccagagccac
     4681 acggattgcc cgtgccgcag aacacctgct caccatcaaa cccgccacgc ctgaagcact
     4741 caacgaatgg aaattaaaac ttgaaggaat tgccagggaa atagatgcct gttttcttcc
     4801 agaaaaactt ccttcagaac tggttgaatt tcgtcagaaa attgtgcttg aaatgatgcc
     4861 aaaaacctcg ggctgaagac gacgggcttg gggttgaaga ctcgaagact cggggctgaa
     4921 gacgacgggc ttgaggttga agaaaacggg ctcaataccc tgagcccaaa gttcttcagc
     4981 ccaatttctt cagcccaatt tcttcagccc aaggattttt cagccccaac ttggtgtacc
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttcttcagcc caatttcttc
     5161 agcccaagga tttttcagcc ccaacttggt gtacccgtga ccaaacttgg tgtacccgtg
     5221 cccaatttag aggattcaac cgggtatttt aaaccagaga ctgattttgt caggtcaacc
     5281 accttgagac aaatatccaa aattcctttt ccgcagaact aaatcaagta aataaataga
     5341 aattatctat attcaattct gagttgaacc tggcaaaaat agccaaattc gagttcagga
     5401 gactgtcatc tggggaaaac tcagtgagga agaggcttgg tgtacctgta accagacttg
     5461 gtgtacccgt gaccaaactt ggtgtacccg tgaccaaaaa cttggtgtac ccgtgaccag
     5521 aaagtctaac caacttggtg tacccgtgac cagaaacttg gtgtacccgt gaccagaaac
     5581 ttggtgtacc cgtgaccaaa aatcacttgg tgtacccgtg accaaacttg gtgtacccgt
     5641 gaccaaactt ggtgtacccg tgaccaaaaa gtctaaccaa cttggtgtac ccgtgaccaa
     5701 tatttggtgt acccgtgacc agacttggtg tacccgtgac cagttttcaa atcaaattta
     5761 actatatctc tcaccaatgg ttgtttgact tttccacagg gaggtctaaa gagcacttgg
     5821 tgtacccgtg accagaactt ggtgtacccg tgaccagaac ttggtgtacc cgtgaccaga
     5881 gccaaatcca caaccccttt attttcaatg gtttatcctg tggaaaaaaa actcctactt
     5941 tcttaaatac tctgtgtaaa tacatgaaat acaaaataca cacacacaca atcccgcctg
     6001 cgcgcgcgcg tgccgcgcca tgcgcgcgag gcggtcttgg tgtgtgcttg aaaacttgtg
     6061 tgtgtgtgtg tttttctctt tttcaaactt caatcaaaaa cacagttccc actttggcaa
     6121 gcgaggtaac cagccggttt atgctcttcc cagaacaccc tctcgacgac gaaaccacaa
     6181 acctggacgt cattcgccct gaactcaacc tcgaaaagtg gccggcgctc tggcagccga
     6241 tccagtcgaa gaacaaacaa aacaaagtcg tcatcgaacg cgaggtcatg cagctcaaca
     6301 acaagttcct ggcccgcgtc gtggccacgg ccaacagcga atatggcccg ctcacgacgg
     6361 aggatcagaa gctgtactac gcgctgcaaa aggtctggga agacagcggt cgccagcgcc
     6421 gaatcgtgtt ttcacgccga cgcattgcga cgatcttggg caaacaatgg ggatcaaagg
     6481 tatcaaaggc gattaacgat tcactgttcc ggctacgggg cacgctcttc gtctggaccg
     6541 actcgtttta cgacagcacc aaccagaaaa ccatcaaaac cctgggcaca ttcaacatcc
     6601 tctcagacct caaaatcgct gaggaatcgg tggatggcca ccgcacgacc gaggaatgtt
     6661 actgcgaatt tcacgaactg atttacaaaa atttgctcaa cgggtacaca aaacctcttt
     6721 tgtttacaac agttgtgagt tttgaagatg gaattgccca gatgctctac agccacctgg
     6781 acctgatgct ggccaacaac gaccactatg aacgccgaac ccgtgaactc tttgacgatc
     6841 tgcagctcca gggcaacgaa tacaagtttg tctcacgccg taaacgcacg ctggagcgta
     6901 ttttaccgac tttgcaagac aaaccgacct cacgtggagg cgttctgcga ctggtgctcg
     6961 acaaaacccg tggcggcaag gatttcaagc tcattgtgga gcgggtttac ctgcataacc
     7021 ccgaaccagt ggaggatcaa gccccaattt ccgccagcca acccgctcct ccagctcccg
     7081 ttatcacggg ggacgacccg gctgacagcg gggaaacccc cgagcccgcc gaactgattc
     7141 aggccagact ggacctcgat caggaaagcg atgacctggt gcgatatttc ctgaaaacgt
     7201 ttcgcatcaa ccgcaaacac ccccgcccaa ccgagctggc tgaggcccga gagttgattt
     7261 acgagtacca gttgactcgc gaaaaaagcc ggtggattgt ggatttcagt ctcaaggcgt
     7321 cgaaggaaac ccggtttgtt cccaagagtt tcaacgggat tgtgcagtac accgaccggg
     7381 ctctggaagc ctggcaactg aacttgctgg aagctgaaaa cctgcctttg cccctggtcc
     7441 cgccgactcc ggagccggaa gacccgattc tggctgctga aaaggctgaa atcgaggaat
     7501 cacaacggtt agaaacgctt ggcgaagagc ttttttcccg cctgtccgag gccgaacgca
     7561 cgacattggt ggcgtcggag ctggaaaccc tgcgcaagtc cgaacactgg aaagtctatg
     7621 cccggatgga aaaaaacgtc ctccacgagc acatttcgtg gctggttggt cgcaaactgg
     7681 cactgcagga actggggcgg ttgtaatgga aatccaggaa ggaaaatttg aaatcgggtt
     7741 tatttgactt ttacggggtt tttgatggct gggatatgca atgtgttatc aggtaggtga
     7801 aaagacgctc agcggtcaaa taaacgcaat tgagcgctat gctgacaggt gattccaacc
     7861 cggtttttca atcctgggtg aaaaaatcaa tcgaaatcgg gtttcccagt gcgaattcgt
     7921 ttggtctggc tgcgcctggt tttttccgct atccggcgtt cctggctcgc tctggtcggg
     7981 gcggtgggtc ggcgtggcct gggcggttcg agtgaccgca ggatcagttt ccggagtttt
     8041 tcgcgggcat cgtcgagatt gcgggcctga tcgcgggaca actggctggt gatgagcaag
     8101 gtatcacccc ccaccagtcg cgacccggca agggcagaga ggcgttcttt gacgccggtg
     8161 cccagcacgg tgcagccggc cagatcaaat cgcagctcaa cgcaggtcgc agtggtgttg
     8221 acgtgctggc cgcctggacc tgatgaccga acagctttcc aggtcagatt ttcggttgga
     8281 attgtgatga attcgttgat ttcgagaggt tctgccataa gttttttgag gaatagaagt
     8341 cactttattt ttgaaaaaaa atagcaaatt aaagaaaaaa aaatcaatga ttaattctgc
     8401 ttcgagcaga ttttgtattg gagaccagtc agccaaaacc actgagtcag tgccaccggc
     8461 gtgagtgggt aggtcggcgt gcttctcacc caacctgcgc acgcaggtgg tactgacacg
     8521 tcgctatttt cgttgcaatt gaatttttaa attaatttat cccaggatca ttttgccctt
     8581 tggagttgat tgactgagcc ggaggtgttt tgctgtgacc gaaatctgat ctccgcgtgc
     8641 gctgaaaaaa ccttaactgg tttataatga agcagttatg ttaggggcta aatt
//
LOCUS       DAOQDO010000013         8611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4417_length_8611_cov_2.941328, whole genome shotgun sequence.
ACCESSION   DAOQDO010000013 DAOQDO010000000
VERSION     DAOQDO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4417_length_8611_cov_2.941328"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PKZ53_00520"
     CDS             complement(<1..1114)
                     /locus_tag="PKZ53_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38944.1"
                     /translation="MSSLTSKALALIRPTLRGNSQLAVALPIAEALAQRVLLLSISAR
                     ELEAAVCNEEGRRYLQERLTQPDFQRELCRVAKLTLIEIARITVRTQVHPEEVIVKAI
                     ERTYPANLEIRINTTLASVLPRAESERMGVLLERVSGTIVYALCTDGNGEKFLRERFQ
                     QKLFREAVAVAAGIPRNAAGSLTLFKPRRVTPMVLKNELNKVFPDHQIKEQSDLKIRT
                     QVQRVRAEREQFTGLGQDRRKPLLPTETRNMINTVVGRAYLRKASDIRMFYDLSRRQL
                     CHEVCIDGDWHNEKPYPIDVDTYHSIMNLLRQDAKIASLERDVPHDGHIRFDFVLEGR
                     RLAFDGRVNILPKEDTLNRRAELSGVTFRLFRDDEEI"
     gene            complement(1185..1766)
                     /gene="pilO"
                     /locus_tag="PKZ53_00525"
     CDS             complement(1185..1766)
                     /gene="pilO"
                     /locus_tag="PKZ53_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNJ38945.1"
                     /translation="MKNGVKIVIQIVILLVAVATVYGVTHKFVLAPQREQLEKQQRDV
                     TDLRRKNDATDRFRLERDALQQESEAAQARYQKVESWLLKASNTASVLTGVKDAARKN
                     GLQVRVFDNTHPPYVQGINESPLKIHLGGRFQGQLEFLRALAFYPQIILIRTQKLESL
                     PAQSTIVMELSAATPIDLPTKEAVAEANAVVAK"
     gene            complement(1823..3427)
                     /locus_tag="PKZ53_00530"
     CDS             complement(1823..3427)
                     /locus_tag="PKZ53_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38946.1"
                     /translation="MLRIVRYGYETPGVDVAYVEVENRPDQFPFPRLIHIEHHPDAQT
                     ALEAVGPQVAGIETISVCSCSTELLTGVEAAHYRPGQTPGVSECIAEYRLGQTLLLNK
                     ISSHTLTQMVGHSALADTACLITAFRTVYFAELQQLTQVAHIHIAERQIDLFVYDRGE
                     LVFHGTELPEDDHDSFMYSAHLLLTDPNARWRSHLDLIVFSGASQWLQANRLEWLDTQ
                     VQQSAQAQVFAADRLFAVELTDVDAQREVSRAESRFTRTGLPVLLAGAAVSFSIQHGA
                     DFRRLVPSEYVPPKSKGLVAVPQTPGQFAKFAALNLSGLALGAILGVGIVGYQYFQNS
                     AWEKRLTNEKTVELKRESDNRQVEAEAKQLRDLIQALNNRIEVVNRMRNSQQANFEIT
                     NDIWQQIPPGVVLSRLLIVGRDITIEGYVTYSSVVEGQNPANTSGTADSGQTAGGDPQ
                     SSPPKAPSVVYFEDQVDQLSKNLSDSGSYSNVQSKLNQTDDTKALFTVIGTYTGEISS
                     DPLTLQPLPEVVKQAAPEPSKLPVTK"
     gene            4103..8338
                     /locus_tag="PKZ53_00535"
     CDS             4103..8338
                     /locus_tag="PKZ53_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ38947.1"
                     /translation="MGSPLAHNPKTSVIIGSLTQYRHHLRHTDRSFPTASWALRGIMR
                     CGLTILCCVIGLFLFLGALPATAVPSPVADPPVSPLATTQVPRPNGRPGLRVYTTQDG
                     IPQSALLSLAFDRKGYLWVGTQDGAARFNGQTWQVINLPERFVSNYIRTVLGTSDGSV
                     WFGTRNGGLARLKDNQWTTFNASNGLPGNAIYALVETSSQPGQSFLWVGTDAGLVRFD
                     GQSWKQITTADGLPGDTITCLQPAPFPEDPSGLWVGTNRGLVKVINQHVEIVSTSSRL
                     HTQPILALWQSPLSNQRQPLWVATPSSVFRIDDATESELVFPGGTSGKGIRALMTTPA
                     PGGAESLWIATEQGLLQFESGHWTLYDQKIGFPSNAILSLLKPASAQASPTLWVGTGR
                     GLVRLNYTGWRSFGKNDGLPDDEIYSICETRDQTLWIGTENGLARWEANRRTVIQGAP
                     ALPNNRVLCLYETTGLEGDSTLWVGTSGGLVRFHRETWSPVPEIPPGMIRQILETTNQ
                     EGKKEMWVAAQFGLAHAVNGQWTVITRQQGLPSNMVLSVLQTRSADGTPTIWAGTDSG
                     LGRFLNGAWNIFTIQSGLPNNTVRALHESIAPDGTHMLWVGSSGGVARLILNGSQSVW
                     ETLSDLTTPALPNNVIYQIREDSRGRLYLTTNKGVVQLTPPADLKRVPQIHEYALHTF
                     TTEDGLAHDEANSGASCIDQAGRLWVGTVGGMAVYDSHQDPPAHAQSPLLIEEVQLRS
                     NAGHGWGWGSRSEDLAGVQALPVIQVDRQTLDYQYTNLGFQYALLGFFRDAETRYRTQ
                     LVGYDTSPSEWSSDPKREYTNLPSGQYMFRVWAKNYAGMVTGPVSVSFTIRPAPWRTW
                     WAYLFFVATLGGVGYGLVQFRLRSLNQRTLQLEDRVAQRTQELAATIEHLRQSEHEAH
                     QAHQEAIDEKRNAQTAAQEAIEAKNKAIEANRAKTTFLANMSHELRTPLNAILGFAQV
                     MNRKPDRSSEDLENLAVIMRSSEHLLGLINDILDVARLEAGRMTLNEHVFHLPELLKS
                     VEELFRIRAQRKDLQFQFTTSPDLPQYTWGDEGKLRQILTKLLENAFKYTGIGYVAFR
                     ANWSETGVTFEVEDTGQGIRPDELRVIFEAFAQSQDNQKYKEGVGLGLTICEKFVRLM
                     GGTLEVNSQLGEGSTFRVHLPLQPVNRAETMAMKKRKIIGLAPGQPQFRLLVADGKWE
                     NIALLDKMLTPLGFKVILASDGKEAIDHWVSFRPHVAFIDGRLPIWDGYTTTRKIRQL
                     EVDERLSSNLSIRTVILAITSSSFEFSQETLLEAGFDGHLDRPLREETLLQHLAEHLG
                     IQYQYEVDDFPTASPSGNNQPTQLTPAVLSALPQSLVSNLQKALNEGNISVTEDLANQ
                     IETTNPALALEIRQYLKSYQFDELLEIIDHGTR"
BASE COUNT         2148 a         2317 c         2170 g         1976 t
ORIGIN      
        1 gaatttcctc atcatcccga aacagtcgga aggtcacccc tgacagttca gcccggcgat
       61 tgagggtatc ttccttcggc aagatattga cccggccatc aaatgcaagc cggcggcctt
      121 ccagaacaaa atcaaatcga atgtgcccat cgtgcggaac atcacgctcc aggctggcaa
      181 ttttggcatc ctgccgaagg aggttcataa tcgagtgata ggtatcaaca tcaatcggat
      241 atggtttttc attgtgccag tcaccatcaa tacacacttc gtggcagagc tggcggcgtg
      301 acaggtcata aaacatccga atatccgagg ctttgcgcaa ataggcccgg cccaccaccg
      361 tgttgatcat attgcgggtc tcggtgggta acagcggttt gcgtcggtcc tggccaagtc
      421 cggtaaactg ttcgcgctcg gcccggacac gctgtacctg ggtccgaatt ttcaaatcgg
      481 attgttcttt gatctgatgg tcaggaaaaa ccttgttgag ttcatttttc agtaccatcg
      541 gggtcacccg acggggctta aacaatgtca ggctcccggc tgcgttgcgc gggatcccgg
      601 cagccacggc cacggcctcg cgaaacagtt tttgctgaaa tcgctctcta agaaattttt
      661 caccgttccc atcggtacac aaagcataca caatggtgcc cgatacccgt tcgagcagca
      721 cgcccatccg ctcagattcg gcccgtggca acacgctggc cagggtggtg ttgatccgga
      781 tttccaaatt ggctggatag gttcgttcaa ttgctttgac gatcacttct tcggggtgca
      841 cctgagtccg gacagtgatt cgggcaattt caatcagggt gagtttggcc acacgacaca
      901 actcgcgctg aaagtcgggc tgggtcagac gttcctggag gtatcggcgg ccttcttcat
      961 tgcataccgc cgcttctaat tcgcgggcag agattgagag caacaggacc cgctgcgcca
     1021 gggcttccgc aatcggaagc gccaccgcca gttgtgaatt cccacgcaag gttggtcgaa
     1081 tcagagcaag tgctttgctg gtcagcgaag acatacgtat accattcagg gattacaagt
     1141 taagggaaat atcattgatt caatgagctg gccaatgaca gcgtctactt tgcaaccaca
     1201 gcattagctt cggccaccgc ttctttggtc ggcaaatcaa tcggcgtggc agccgacaat
     1261 tccatcacaa tcgttgactg ggctggcaag gactcaagct tttgggtgcg aatcagaata
     1321 atctgggggt aaaaggccag cgcacgcaaa aattccaact ggccttgaaa tcggccaccc
     1381 agatgaatct tgagtggtga ctcattgatt ccctgaacgt agggtggatg tgtattatca
     1441 aaaactctga cttgtaaccc atttttccga gcggcatctt tgaccccagt caagaccgaa
     1501 gccgtatttg aggctttcag gagccagctt tcgaccttct ggtatcgagc ctgggctgcc
     1561 tctgattctt gttggagcgc gtcacgctcc agacgaaacc gatcagtggc atcgtttttg
     1621 cgccgaaggt cagtcacgtc acgctgttgt ttttcaagct gttcgcgctg gggtgccaag
     1681 acaaatttat gggttactcc atacacagta gccacggcca ccagcaggat cacaatctgg
     1741 attactattt tgactccgtt tttcataggt caacagtgtc ggacagagtg aactcaactc
     1801 ggttcaggca aagtaagaca ggttatttgg tcacaggcag ttttgatggt tcgggagcgg
     1861 cctgtttgac aacctctggc aaaggttgaa gcgtgagcgg atcagatgaa atttctccgg
     1921 tatacgtccc gatcacggta aacagggctt ttgtgtcatc ggtctggttc aatttcgact
     1981 gaacattgct ataactccca ctgtcgctca ggttctttga caactggtca acctgatcct
     2041 cgaaatagac caccgaggga gctttcggcg gtgaactttg cgggtcaccg cctgcggttt
     2101 gacctgaatc agcggtccca gacgtattgg ccgggttttg gccttccacc acggacgaat
     2161 aggttacata gccttcaatc gtaatatcac ggccaacgat caagagcctg gacaggacca
     2221 cgccaggcgg gatttgttgc cagatatcat tggtaatttc aaagttggcc tgttgtgaat
     2281 tgcgcattcg attcacaact tcgatgcgat tgttcagggc ctggatcaaa tcacgaagct
     2341 gtttggcttc ggcctcaacc tggcgattgt cggattcccg ttttaattcg actgtctttt
     2401 cattggtcag tcgtttttcc caggctgagt tttggaaata ctgatagccg acaatgccca
     2461 ccccaaggat ggccccaagc gcgagacctg acaggttcag tgcggcaaac ttcgcaaact
     2521 gtccaggtgt ttgggggacg gccaccaacc ctttactttt tggagggaca tactctgagg
     2581 gcaccaagcg tctgaaatcc gcaccatgct ggattgaaaa actgacggca gcccccgcca
     2641 gcaatacagg caatccagtc cgggtaaacc ggctttccgc ccgactgact tcacgttgag
     2701 catcaacatc agtcaattca accgcaaaca gccgatctgc ggcaaaaacc tgagcctgcg
     2761 cactttgttg gacctgtgtg tcaagccact caagccggtt ggcctggagc cactgactgg
     2821 cacctgaaaa cacgatcagg tccaggtgac tgcgccaccg ggcgttggga tcagtcagta
     2881 acagatgggc gctgtacata aaactgtcgt gatcatcttc gggcaattct gttccatgaa
     2941 aaacgagttc ccctcgatca tagacaaaaa gatcaatctg gcgttcagca atatggatgt
     3001 gggcaacctg ggtcaattgc tggagttcag caaaatagac ggtgcgaaaa gctgtaatca
     3061 aacaagccgt atcggccagt gctgaatgac cgaccatctg ggtcaacgta tgcgacgaaa
     3121 ttttattgag taagagtgtc tgacctagcc gatattcagc aatgcattcg gaaacacctg
     3181 gggtttgacc tggccggtaa tgagcagctt caacaccagt gagcaactca gtcgaacacg
     3241 agcagacact gatggtttca ataccagcca cctgtggccc cacggcttcc agtgctgttt
     3301 gggcatctgg gtggtgctca atatgtataa gtcgcggaaa tggaaactgg tcgggtcggt
     3361 tttcgacttc gacataagcc acatcaacgc caggggtttc atatccgtat ctgacaattc
     3421 gcagcatagc cttctcgctt tatgaatttt tgatgtgttg ggtaaccacc tctgttggta
     3481 cagacgattg ctcgccagga gcagatggca aaaagccaaa attcttgcct ggatccagcc
     3541 tgcatcagag atcttgatca acccaaaaac aacaaatttg aacatcccaa tttctgcagg
     3601 taccacaatc acatcttcaa ggcacactca tccaaccgga acgttgatag ttgaactgac
     3661 actgcacacc tgactgtctc tgcttatttc agaaattgga agcggaaaaa tacagaaaaa
     3721 tcgcatcatt ttccaatccc aaggtttgaa tttatcgctt tttttgaaaa aatttcgctt
     3781 tctcaatcaa atgaacccca gagtcccaca gttccctctg gccgagccac cacaaggaac
     3841 tcatcccacc cccgcttgaa atctaaatgc caggtgtcgg aatcaaagtc tgaacgcggc
     3901 tgtacccgct ctgcagattg aatcactcaa cccgtaacga cctcccagga tttttcagtt
     3961 ttacccacga atgccactca gggcctgggt ttgtctcgaa ctactattca gcactcagaa
     4021 tttgacctaa agtgaggaac cggtgttttt caaggggcaa gcacctcctg ttgtacctga
     4081 aacatgccgg gcgcggactt caatgggcag ccccctcgcc cacaatccca aaacatcagt
     4141 gatcattggt tcactcaccc aatatcgcca ccatctgcgt cacaccgacc gatcatttcc
     4201 aacagcatcg tgggcattgc gaggaattat gcgctgcggt ttaaccattc tgtgttgtgt
     4261 gattggactc tttcttttcc tgggtgccct gccagccaca gccgtaccgt caccggtcgc
     4321 tgacccacca gtatcaccac tggcgacaac tcaagtccca cgcccaaatg gacggcccgg
     4381 attacgagtt tacacaactc aggatggaat cccgcaaagc gctctgcttt cgctggcctt
     4441 tgatcgcaaa gggtatttgt gggttggaac tcaggatggt gccgctcgat ttaatggtca
     4501 gacctggcag gtcatcaatc tcccagaacg gtttgtttcc aattatattc gcacggtgct
     4561 cggcacaagt gatggaagtg tctggtttgg aacccgcaac ggagggctgg ctcgactcaa
     4621 agacaatcag tggacaacct ttaacgcatc gaatggattg ccaggcaatg ccatttatgc
     4681 cctggttgaa accagttctc aaccaggtca gtcattcctc tgggtaggaa cggatgccgg
     4741 actggtccgc tttgacgggc aatcctggaa acaaatcacc actgccgacg ggctgccagg
     4801 cgataccatt acctgccttc aaccagcacc atttccagaa gatccatccg gcctttgggt
     4861 tggcaccaac cggggactgg tgaaagtcat caaccagcac gtcgagattg tttccacctc
     4921 ttcgcgcctt cacactcaac caattctcgc cctctggcaa tcccccctct caaaccagcg
     4981 ccagccatta tgggttgcca cgccgagcag tgtgtttcgg attgatgacg ccaccgaatc
     5041 ggaacttgtc tttccgggcg gaacctctgg gaaaggcatc cgcgctctga tgacgacacc
     5101 ggcccctggc ggggcagaat ccctgtggat cgcaacagag caagggctgt tacagtttga
     5161 aagcggtcac tggacgctct atgaccagaa aatcgggttc ccatccaacg ccattctgtc
     5221 attgctcaaa ccggcctcag cccaggcttc cccaacgctg tgggtcggaa ctggccgggg
     5281 gctggtccgg ttaaattata ctggctggcg gtcctttggc aaaaacgatg gacttccaga
     5341 cgatgaaatc tacagcattt gtgaaaccag agatcaaacg ttgtggattg gcacggaaaa
     5401 cggcctcgcc cgctgggaag ccaatcggcg gacggtcatt caaggggcgc cggcgcttcc
     5461 caacaaccgg gttctgtgcc tctatgaaac aaccgggctg gaaggagatt caacactctg
     5521 ggttggcacc tcaggtggac tggtccgatt tcaccgcgaa acctggtccc cggttccgga
     5581 aattccaccc ggaatgattc gacaaatcct ggaaacgaca aatcaggaag gaaaaaagga
     5641 gatgtgggtg gccgcccagt ttggcctcgc ccacgccgtc aatggtcaat ggacggtgat
     5701 cactcgccag caggggttgc ccagcaatat ggttttaagt gtgcttcaaa cccggtcagc
     5761 cgatggaacc ccaaccatct gggccggaac cgatagcgga ctgggtcgct tcctcaacgg
     5821 cgcctggaat atctttacca ttcaatctgg tctgccaaac aacaccgttc gggccctgca
     5881 cgaatcaatt gcccctgacg gaacacatat gctgtgggtt ggctcctcag gcggggtcgc
     5941 cagactcatc ctcaatggat ctcaatcggt ctgggaaaca ctttctgacc tgaccacgcc
     6001 agcactgcca aataacgtca tctatcagat tcgagaagac agccgtggtc gcctgtatct
     6061 cacaacgaac aaaggagttg tccagttaac ccctccggcg gacctcaaac gcgtaccgca
     6121 gatacacgaa tatgccctgc ataccttcac cacggaagac ggactggctc atgacgaagc
     6181 gaattccggc gcttcctgca ttgaccaggc tggccggctc tgggttggaa ccgttggagg
     6241 aatggccgtt tatgattcac atcaagatcc accggcccat gcccaatccc cactgctcat
     6301 tgaagaagtc cagcttcggt caaatgcggg tcacggatgg ggttgggggt cacgttctga
     6361 ggatctcgct ggtgtgcagg cactccctgt tattcaagtt gatcgccaaa cccttgacta
     6421 ccagtacacc aacctggggt ttcagtatgc ccttctgggg tttttccggg atgccgaaac
     6481 ccgttaccgc acccaactgg tcggctatga caccagtcct tcagagtgga gcagtgatcc
     6541 caaacgggaa tataccaatc tgccaagcgg tcaatacatg tttcgggtgt gggccaaaaa
     6601 ttatgcaggt atggtcacag gcccggtctc ggtcagtttt actattcgac cggcgccctg
     6661 gagaacctgg tgggcgtatc tcttttttgt ggccacgctg ggtggagttg gatatgggct
     6721 ggttcagttc cgattgcgct cactcaacca acgaaccctg caacttgaag accgggtcgc
     6781 ccaacgaacc caggaactgg cagccactat cgagcacctg cgccagtccg aacacgaagc
     6841 ccatcaggcc catcaggaag ccattgacga aaaacgcaat gcccagaccg ctgctcagga
     6901 agccatcgaa gccaaaaaca aagccattga agccaatcgc gccaaaacta catttctggc
     6961 caatatgagc cacgagttgc gaacgcccct caacgccatt ctgggatttg cccaggtgat
     7021 gaaccgcaaa cctgaccgga gttcggaaga tcttgaaaat ctggcagtga ttatgcgcag
     7081 cagtgagcat ctgcttggct tgatcaatga cattctcgat gtcgcccgac tcgaagctgg
     7141 ccggatgaca ctcaatgaac acgtgtttca cctgcctgaa ttgctcaaaa gtgtcgagga
     7201 attgtttcgc attcgcgccc agcgcaaaga tcttcagttc cagttcacaa cctcgcctga
     7261 ccttccacaa tacacctggg gcgatgaagg aaaattgcgt cagatcctga caaaactgct
     7321 cgaaaatgct tttaaataca caggcattgg ctatgtggcc ttccgggcaa actggtcaga
     7381 aacgggcgtc acctttgaag tcgaagacac cggtcagggg attcgtccag atgaactgcg
     7441 ggtcatcttc gaagcctttg cccagagtca ggacaaccag aagtacaaag aaggcgtcgg
     7501 cctcgggcta accatttgtg agaaatttgt ccggttgatg ggaggcacgc ttgaagtcaa
     7561 tagccagtta ggcgaggggt caaccttccg ggttcacctg ccgctccagc ccgtcaaccg
     7621 ggcagaaacc atggccatga aaaaacgcaa aattattgga ctggcgccgg gacaacccca
     7681 gtttcggcta ctggtcgcag atggaaaatg ggaaaatatc gccctgctgg acaaaatgct
     7741 gaccccactt gggtttaagg tcattttggc cagtgatgga aaagaggcaa ttgatcactg
     7801 ggtcagcttc cggccccacg tggcattcat tgacggacga cttccgatct gggacggata
     7861 caccaccacc cgcaagatca gacaacttga ggttgatgaa cgactgagtt caaatctttc
     7921 cattcgaacc gtaatcctgg caattacttc gagtagtttt gaatttagcc aggaaacact
     7981 gctcgaagca gggtttgatg gtcacctgga tcggccactg cgtgaagaaa ctttgctcca
     8041 gcatctggct gaacacctgg gcatccagta tcaatatgaa gtagatgatt tcccgacggc
     8101 ttccccatca ggaaacaacc agccaaccca gttaacacca gcggttttgt cagctttgcc
     8161 tcaatcattg gtttcaaatc tgcaaaaagc attgaacgag ggcaatatct cggtaaccga
     8221 agatctcgcc aaccagattg agaccaccaa cccggcactg gcactggaaa tccgccagta
     8281 cctcaagagc tatcagtttg atgaactgtt agagatcatt gaccacggaa cccggtagcg
     8341 gcaggaatga agaatgaaga atgaagaatg aagaatgaag aatgaagaat tgacattatc
     8401 ggagataaac cgggccaccg aaaaaaggat ggcgtcaagt cagtaccacc tgcgtgagcg
     8461 ggtgggttgc cacggcaaga aagctccact tttggaaaat gatgaacccc gataatgtcg
     8521 aatgaagaat tgacaatgaa gaattgagaa agcggctagt tgtactgaca actcacgttg
     8581 ttttacgtat ccctggtaat tctttgaaaa a
//
LOCUS       DAOQDO010000014         8555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4462_length_8555_cov_3.638941, whole genome shotgun sequence.
ACCESSION   DAOQDO010000014 DAOQDO010000000
VERSION     DAOQDO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_8555_cov_3.638941"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKZ53_00540"
     CDS             complement(<1..330)
                     /locus_tag="PKZ53_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ38948.1"
                     /translation="MRFANQSLRYHTILRPIRPVQSRRSRGFSLIELLIVVAIIGIIA
                     AIAVPNLQASRRAANQASAVSTLRTYHSAEMTFRNGNGFLVDFVELKNLTAGGIDEVL
                     GSQHNNVP"
     gene            807..1550
                     /locus_tag="PKZ53_00545"
     CDS             807..1550
                     /locus_tag="PKZ53_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ38949.1"
                     /translation="MQCQECENLLSDYLDHAIEPHLRTEMEVHLAACPGCTALHNELC
                     QLLAASELLLPQVSGPTIRAHRVPQPGHLGAIPASPHQQVFLRIKQLLFERYVEIKLT
                     IPQFSALVGILGGLVLFGVWSLQNPAMLNSQGESRPGLASPASPGTSNTVLIPTTQLV
                     SHSRNYQAERVVLTESITRLSKALETRKVDWDPELRRVFERNMALIDQSLLECEVALR
                     SNPHDFGAEEMLIAAYREKLRLLQEFAAI"
     gene            1678..2343
                     /locus_tag="PKZ53_00550"
     CDS             1678..2343
                     /locus_tag="PKZ53_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNJ38950.1"
                     /translation="MHVPIQGSASGLLIRSYRVDPQSARFSLRGMKGPITITTAVKDE
                     IKVKAESQTGQVKIADTQLDNFVQLSVADDRAGLVKFEVVVPPTCNLEIKCLDGAISI
                     QDAMGSISAETTDGDITLKNLRCAQVNACSTSGRIFFDGEILPKGNYSFQSFNNLIDL
                     TVPAAAPFQLDGTAFTADIDLGGFVVTNFAPGSKQISGVHRQGNARLYLMTHRGQIRF
                     HKK"
     gene            complement(2564..6082)
                     /locus_tag="PKZ53_00555"
     CDS             complement(2564..6082)
                     /locus_tag="PKZ53_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ38951.1"
                     /translation="MKRYLALLSLVLFAASVIYTSIGRMQSGSTFQSPANSRPVVTQL
                     EEEQPMILPDGYISASDRHKVMVSADDAALYGELTASGGVLAEENYGSFRLLTINSET
                     LERVQGRAEVRDDMNVITLNGYQLDTTAPEATLSSLPVEFVRTKDAETRAVQSDKGSR
                     TKLFIVQFSGPIQDNWYKKLVKTGARVVSYVATNGYIVEATNGQIEKLDAMRQDAKNG
                     VQYVGFYEPAFRTTSVLRNAYTKGMIGSVDITVQVVNNHRADETIRQLTEMASRVIRQ
                     AHPVLGYQNITLTVPASQLAEISQIDNVFGVEPFYEPRLLDERQGQIMAGNITGSTVN
                     GPGYLTWLQSKGFSGTGQFDFVVDVTDDGIDRGSTTDVNQEYKVDGSASGASRLVYNN
                     NYTGDASADGGGGHGNLNASVIFGYNNLTGTAYEDATGHNYGLGIAPWVKVGNTKVFS
                     NGSAATFTQSATVRLKAAYTGGARISNNSWGYTSGNSYNTDSQEHDRIVRDADSAATG
                     NQELTVVFAAGNSGSGANTVRPPATAKNVITVAAFENDRQTGTDGCATDNTGANNVND
                     VIGFSSRGPCSDGRVKPDLGAPGTHIQGAASRSTAYSGGGVCNKYWPTGQTLYAWSSG
                     TSHSAPGVAGAAALVRQYFINQGWGTPSPAMLKAYLANSTRYMNGVGANDTLPSNNQG
                     MGSVNLGTGFDGVQRVNLDQTRVFAATGETYSTSGTIANTSKPFRVTLAWTDAAGPTT
                     GNAWVNNLDLEVTVGTVTYKGNVFAGANSTTGGSADLRNNMESVFLPAGTSGNVSVRV
                     IATNIAGDGVPGNADTTDQDFALVIYNATTGGTPTPDYTVSTSPTSGTVTAGSSTTST
                     VTVGSVNGFTGTVNLTASGLPGGASASFSPASISSGAGTSTMTISTTSATVAGAYPVT
                     ITGTSGTTTRTASYTLNVTASGGGGGTQTVTGSNTTAVAIPDNNTTGITSVINIPTSL
                     TIQSVSASVNITHTYRGDVQIELISPAGTTTTLKSTSSSDSAANIVATYTPTAFAGQN
                     SAGNWTLRVKDLASADTGTLNNWNLSITGTTAGGGGTTGDFTLSLETTTGTWRRGSTY
                     TRTVYVNRTGGHSSPVTLSYSASKTGLFSSVTASVNPVPATGTSSVMTFRVSTTAPTG
                     SATVTIIGTDGNNVQRNTTLSLSITT"
     gene            complement(6515..7990)
                     /gene="gatB"
                     /locus_tag="PKZ53_00560"
     CDS             complement(6515..7990)
                     /gene="gatB"
                     /locus_tag="PKZ53_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNJ38952.1"
                     /translation="MNLREKYEPVIGLEIHAQLKTNSKIFCGCATHFDALPNANTCPV
                     CLGLPGALPVLNREVVEKAARAARALNLQITPTSIFARKNYFYPDLPKGYQISQFDRP
                     FSINGFVEIPTTERDHHGRPIEWQLKRFRIQRLHLEEDAGKSLHEGMPDSAHTSYVNL
                     NRSGMPLAEIVSEADFRSSWEAYDYVQYLRRSLLYVDACDGNMEEGSLRCDANVSVRL
                     RGTEAFGTRTEIKNLNSFRFIQRAIDYEIDRQMELLENGGRVIQETRLWNEKEGRTYT
                     MRSKEEAHDYRYFPEPDLPPLHLRPEWIAEVDTRMPELPEARRRRFISEYSLSFDDSM
                     LLTSTREIADFYEATAKAAGNTRAAANWITSELLARMKVDDLQNSPVSPAALGEMIKL
                     IDDGTISGKMAKEIFDQMFKTGKSAAVLVKEMGGGQISDASQLETIIADAITANPKQA
                     ADYRAGKQALLAFFVGQVMKVTKGKANPQLVNDILKAKLAE"
     gene            complement(8033..8407)
                     /locus_tag="PKZ53_00565"
     CDS             complement(8033..8407)
                     /locus_tag="PKZ53_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ38953.1"
                     /translation="MKRILAADDDPAIRFLIQNILRGKFELTVVGDGEQAIQCLQQDT
                     GWDCLLLDIEMPNKNGLEVLRFAKGNPKLHHIPVIILTGVTDIRTQSSALREGVVTYF
                     RKPFSPSQLLSVITATTRNYST"
BASE COUNT         2064 a         2171 c         2107 g         2213 t
ORIGIN      
        1 gggtacatta ttatgttgac ttccaagaac ctcatcaatc ccacccgcag taagattttt
       61 taactcgaca aaatcaacaa gaaatccatt tccatttcga aatgtcattt cagcactatg
      121 gtaagttcga agcgttgaga cggctgaagc ctgattcgcc gcccgacgtg aagcttgcag
      181 attgggaacg gcaatcgcgg cgataattcc aatgatggcc accacgatta acaactcaat
      241 taaggaaaac ccacgtgagc gccttgactg aaccgggcga atcggtcgca ggatggtgtg
      301 gtatctcaaa gattgatttg caaaacgcat atctatcaag cctctctgct atacaagtag
      361 caagtgtgac gaaaccaggg tcggttgtgg tgttgaaggg cagttgatga aagaaatcag
      421 aaattctgtt tctgataagc aactggtatt ccacaacaac tctcctgtga tgaggaaaga
      481 gactaacctg ctgattctac acagatacac aactgagaac cgacgaaaaa tacagaaatc
      541 gaaggggata atgacaggaa atgtaaactc ctggtcgtca taactgacaa ttcttgttat
      601 cattttccgt agcctgtgac tgaagcctga cagtctccaa agagcaacgg ttctggaacc
      661 aagggcatcg caacgccggc ttcactctgt caagatcttc ggaaagaacc acctgttttc
      721 tcaattcaca ttaccagcct ctggctatat tctgctttga actctcgtca tttgaattta
      781 ttcatgcctg gagtttctca tcctctatgc aatgccaaga atgtgagaac ttactctctg
      841 attacttaga tcatgcgatt gaacctcacc tcagaacaga gatggaggtt cacctcgctg
      901 cctgccctgg atgtacggcc ctgcacaatg aactatgtca acttctggcc gccagtgaat
      961 tattactccc ccaggtcagt ggaccaacca tccgcgctca tcgtgttccc caaccaggtc
     1021 acctgggtgc aattccagcc tcaccacatc agcaagtctt cttgcgaatc aaacaacttc
     1081 tgtttgaacg ctatgtggaa attaagctca ccattccaca attttcagcg ttggtcggta
     1141 ttctcggggg actggttctg tttggagtct ggtcgcttca aaatccagcg atgctcaaca
     1201 gccagggaga atcacgccca gggctcgcca gtccagcctc gcccggcacg tcaaataccg
     1261 tgttgatacc cacaacacaa ctggtatcac attctcggaa ttatcaggct gaacgggtgg
     1321 tgctcactga atcaatcacc cggttgagca aagcacttga aaccaggaaa gtggattggg
     1381 atcctgagct gagacgggta tttgaacgaa atatggcgct cattgaccaa tcgcttttgg
     1441 aatgtgaagt tgccttacga tcaaacccac acgactttgg cgccgaagaa atgctgattg
     1501 cggcatatcg cgaaaaactg cgactccttc aagaatttgc tgcaatttga actgaggggg
     1561 tgtggagggc ttgtggaata cgtagcgtgt caaccggttc gcacgtcatt aaaaccaagg
     1621 ttcatcctgt ttgggtggtg gtgtttttgc ttgtgcgttg ctttctctgg ttttggattg
     1681 cacgttccga ttcaaggatc agcgtcgggg cttctgatcc gatcctatcg ggttgaccct
     1741 caatcagcgc gcttttcgct ccgtggaatg aaaggtccaa tcacgattac aactgccgtc
     1801 aaagatgaaa ttaaggtgaa agccgaaagt cagaccgggc aggtaaaaat tgccgatacc
     1861 cagcttgaca actttgtcca actttcagtt gctgatgatc gggcagggtt ggtgaaattt
     1921 gaagtcgtgg ttccaccaac ctgtaacctg gaaattaaat gcctcgatgg tgccatctca
     1981 attcaggacg cgatgggctc aatcagtgct gaaaccactg atggcgatat taccctgaaa
     2041 aatctgcgct gcgctcaggt caacgcctgt tcaaccagtg gtcgaatctt ttttgacgga
     2101 gaaattttgc cgaaaggcaa ttactcgttc caatcattta acaatttaat tgatttgacg
     2161 gtaccggctg ccgctccgtt tcaacttgat ggaacggcat ttacggctga tattgatctc
     2221 ggggggtttg tcgtgaccaa ctttgcccct gggagcaaac agatttctgg tgttcatcga
     2281 caagggaatg ctcgacttta cctgatgacc caccgtggcc aaattcggtt tcataagaaa
     2341 taagtcagta gccacgtgtt ggaagcttag agtttctgtc aagtgtactt tgttctgatg
     2401 caataattta tcgggattcc tccaaagaaa atcaggtctt ttgaagacct tacctactga
     2461 ctacggactc ttaactaaat tctagcggct gtaaacaaaa aaagagagcg ggtgacctgt
     2521 caagtcaccc gctctttgct tcgaggtgag gatcaatcag caactaagtt gtaattgaca
     2581 ggctcagggt cgtgttgcgc tgaacattat ttccatctgt cccaatgatc gtcaccgtcg
     2641 ccgagccagt tggagcagtg gttgaaactc tgaaggtcat cacactgctg gttccagtgg
     2701 ctggcaccgg gttgacagat gcggtcacac ttgagaacaa cccagttttt gaggcactgt
     2761 atgacaaggt cacgggtgat gaatgaccac cagtgcggtt gacatacacc gtgcgggtat
     2821 aggtgctgcc ccgccgccac gttccggtcg tggtttccag cgacagggta aaatcgccag
     2881 tcgtcccgcc gccaccggct gtcgttcccg tgatggaaag gttccagtta ttcaaagttc
     2941 cagtgtcggc tgacgccaga tctttcaccc gcaaggtcca gtttccggct gaattctgcc
     3001 cggcaaaggc tgttggtgtg taggtggcaa cgatatttgc cgcactgtca cttgaactgg
     3061 tgctcttcag tgtcgtcgtt gttccagccg ggctaatcaa ttcgatttga acatctcccc
     3121 ggtaggtgtg ggtaatgttg acagaagcgc tgacactttg aatcgtcagg ctggttggaa
     3181 tattgatcac actggtaatg ccagtcgtgt tgttatctgg aatggcaact gccgtggtat
     3241 tgctaccagt taccgtctgc gttccaccac cgccaccgct cgcggtgaca ttcaaggtat
     3301 agctcgcggt gcgagtggtc gttccgctcg tcccagtgat cgttacggga taagcaccag
     3361 caaccgttgc cgatgttgtg ctaatcgtca tggttgaagt accagcccca gagctgatcg
     3421 aagccgggct aaatgaagcc gaagccccac ctggcaaccc agaagcagtc aaattcacgg
     3481 tgccggtgaa cccatttaca ctccccaccg tcacggttga agttgttgag cttccagccg
     3541 tgaccgttcc actggttgga gaagttgaaa ccgtgtaatc cggagttggc gtgccaccag
     3601 tcgtcgcatt gtaaatcacc agggcaaaat cctgatcggt ggtgtcagcg ttgccgggaa
     3661 caccatcacc cgcgatgttg gttgcaataa cacgcacgct cacgttccca ctggttccgg
     3721 ctggaaggaa aacagattcc atgttgttgc gcaggtcggc gcttccaccc gtggttgaat
     3781 tagccccagc aaacacgttg cctttgtagg taacggtgcc aactgtcact tcgagatcaa
     3841 ggttgttcac ccaggcgttt ccagtcgttg gaccagccgc atcggtccag gccagcgtta
     3901 cccggaaggg tttcgaggta ttggcgatgg tgccggaagt tgaataggtt tcacctgttg
     3961 cggcaaacac acgggtttga tccaggttca cgcgctgcac tccatcaaag cccgttccca
     4021 aattcacact gcccattcct tgattgtttg atggcagggt gtcattggcg ccaacgccat
     4081 tcatatagcg ggttgaattg gccaaatatg ctttcagcat ggccgggctt ggagttcccc
     4141 agccctggtt gatgaaatat tgacgaacca gtgcggcagc accagccacg ccaggtgctg
     4201 agtgagatgt tccggatgac caggcgtaca acgtctgccc ggttggccag tatttattgc
     4261 aaactccgcc gccagagtat gcggttgacc gtgacgccgc accttgaatg tgcgttcctg
     4321 gtgctcccag gtctggtttt acacgaccgt ctgagcacgg accacggctt gaaaagccaa
     4381 tcacgtcgtt gacgttgttg gcgcccgtgt tgtcagtggc acatccatct gttccagtct
     4441 ggcgatcatt ttcaaatgcc gccacagtga tgacgttttt agcggttgcg ggtgggcgca
     4501 cggtattggc gccagagcca gagtttccag ccgcaaacac aaccgtcaat tcctggttac
     4561 cagtcgcggc agaatcagca tcgcgcacaa tccggtcgtg ctcctgtgaa tccgtgttat
     4621 atgagtttcc agaggtgtag ccccagctat tgttgctgat gcgagcgcca cctgtgtagg
     4681 cagctttcaa ccgaactgtg gctgactggg taaacgtcgc cgcactaccg ttgctaaaca
     4741 ctttggtgtt tccaactttc acccacggcg caataccaag gccataattg tgacccgtgg
     4801 cgtcttcata ggccgtccca gtcaaattat tgtagccgaa aataaccgag gcattcaggt
     4861 ttccatggcc gccgccgcca tcggctgaag catcgccagt atagttattg ttgtagacca
     4921 gacggctggc gccggaggca gaaccatcca ctttgtattc ctggttcaca tcggttgtgg
     4981 atccacgatc aataccgtca tcagtgacgt ccacaacaaa gtcaaactgc ccggttccag
     5041 aaaaaccttt tgactgcaac caggtcaggt agcccggacc attgacggtc gaaccagtga
     5101 tgttgccggc cataatctgg ccctgacgtt catcaagcaa tcgcggttca taaaaaggtt
     5161 caaccccaaa cacgttatca atctgactga tttcagccag ttgggaagcc ggtacagtca
     5221 atgtgatgtt ttgatatccc agcaccggat gggcctggcg gatcacgcga ctggccattt
     5281 cggtgagctg acgaatggtt tcatcagcgc ggtggttgtt cacaacctgg accgtgatat
     5341 caaccgaacc gatcatgcct ttggtgtatg cattgcggag cactgatgtg gtgcggaagg
     5401 caggctcata gaacccaacg tattgcaccc catttttggc atcctgacgc atggcatcaa
     5461 gcttctcgat ctgtccattg gtcgcttcaa caatgtatcc gttggtggcc acatagctca
     5521 caacccgagc acccgttttg accagctttt tgtaccagtt gtcctggatc ggacctgaga
     5581 actggacgat gaacagtttg gtgcggctgc ctttgtcact ttgaaccgct ctggtttcag
     5641 catctttggt gcgcacaaat tcaactggca ggcttgacaa cgtcgcttcg ggcgccgtgg
     5701 tgtctaactg gtagccgttg agtgtgatga cgttcatatc atcgcggact tcagcccgcc
     5761 cctgaacacg ctccaacgtt tcagaattga tggtcagtag gcgaaagctg ccatagtttt
     5821 cttcggccag aacaccgccg gaggcggtca attcgccata cagagctgca tcatcggcgg
     5881 aaaccatcac tttgtgacgg tcgctggcac tgatatagcc atcgggcaaa atcatgggct
     5941 gttcttcttc aagctgtgtc accacgggac ggctgttggc gggtgactga aaggtgcttc
     6001 ctgattgcat gcgtccaata ctggtataaa tgacgctcgc cgcaaacagg accagactta
     6061 atagggcaag gtagcgcttc attggatgaa ttccctctct cagagattgc gttgtgagtt
     6121 gttgtaatga ttttgaaagg taagaaactg acgtaatccc ttaagcaatt atcacgccag
     6181 agcttcatct ttttccttca cgacagcaca aaatcaggca aactattgga atacaagcca
     6241 gttacccgcc aaatatttta agattgaatt cagattgaag cttcagccag gaagaataaa
     6301 atgcatactt actacacatc aaatcaggtt acaatttttg tcacacgacc taaccttctt
     6361 tttttcaata ttttgaatta aacgtaaagt tcatcaagct actgtcaatt gatttgacag
     6421 aactctctct taaaagcaca aaagccacct gagacaggtt ttctgctgtc tcaggtggct
     6481 tttggcgact gactacaaac tggcgtcact caaattattc agcaagtttg gctttcaaaa
     6541 tatcgttgac cagttgggga tttgcttttc ctttggtgac tttcatcacc tgaccaacaa
     6601 agaacgcgag cagtgcctgt ttcccagccc ggtaatccgc ggcctgtttt ggattggcag
     6661 tgatggcgtc ggcaatgatg gtttcaagtt gcgatgcatc tgaaatctgg ccgccaccca
     6721 tttccttgac cagaacggcg gctgattttc cagtcttgaa catttgatcg aaaatttctt
     6781 ttgccatctt gccactgatg gtgccgtcat caatcagctt gatcatttct cccaacgccg
     6841 ccggggagac cggggagttt tgtaaatcat caactttcat tcgggcgagc aattcactgg
     6901 tgatccagtt ggccgctgcc cgggtatttc cagcggcttt ggccgttgct tcataaaaat
     6961 cagcaatttc gcgcgtgctg gtcagcaaca tactatcgtc aaacgacaaa ctgtactcgc
     7021 tgataaaccg acgccgacgg gcttctggta attccggcat tcgggtatca acttcagcaa
     7081 tccattcagg acgcaggtga agcggtggca aatcaggttc cgggaaatac cgatagtcgt
     7141 gggcttcttc cttgctgcgc atggtgtagg tgcgaccctc tttttcattc cacaaccggg
     7201 tttcctgaat cacgcgccca ccattttcta aaagctccat ctggcgatca atttcataat
     7261 caatcgcccg ctgaataaat cggaatgaat tcaggttttt aatttccgtt cgggtgccaa
     7321 acgcttctgt tcctcgtaag cgaaccgata cgttggcatc acagcgcagg ctgccctcct
     7381 ccatattccc gtcgcaggca tccacataga gcaggctccg acgcaaatac tgtacatagt
     7441 cataggcttc ccaactgctg cggaaatcgg cttcactgac aatttcagcc agcggcatcc
     7501 cgctccgatt gagattgaca taggaggtat gggctgaatc gggcatgcct tcgtgcaggc
     7561 ttttgccggc gtcttcttca aggtggagac gctggatgcg aaatcgcttg agctgccatt
     7621 caatcgggcg tccgtgatga tcgcgttcag tggttgggat ctcgacaaac ccattgattg
     7681 aaaacggtcg gtcaaactgt gagatttggt agcccttggg taagtcggga taaaagtaat
     7741 tctttcgagc aaaaatcgaa gtgggcgtaa tctgcaagtt cagggcccgt gctgcccgcg
     7801 ccgctttttc caccacttcg cggttgagca ccggaagagc gcccggcagg ccaagacaga
     7861 ccggacaggt attggcgttt ggtagtgcat cgaaatgggt cgcacaaccg cagaaaatct
     7921 tcgaattggt ctttaactgg gcatggattt caagcccgat gacaggttcg tacttctctc
     7981 tcaggttcat ctcgcaaccg cttcctcact ataagatatg ggcgtttaaa cgctaagttg
     8041 aataatttct ggtggtggcc gtaatcactg acaggagttg tgatggtgaa aacggcttgc
     8101 gaaagtaggt caccacccct tctctcaacg cactgctttg ggttcgaatg tccgtcacac
     8161 ccgttaaaat aatcactgga atatggtgta actttggatt tcctttcgca aatcgtaaga
     8221 cttccagccc atttttgttg ggcatttcaa tatcaagcaa caggcaatcc cacccggtat
     8281 cttgctgcag acattggatg gcctgttcgc catcaccaac cacagttaac tcaaatttgc
     8341 cacgcagaat attttgaatc aaaaatcgaa ttgccggatc atcatcggca gccaaaatgc
     8401 gtttcatagt actcctttag acaggatcaa tgatctaaag ctgatcgaat gcctgagtat
     8461 ctggtttcaa tcagcagata ttagtgaata tgtttgcttt ggttgtcccg attcattcga
     8521 tcgagaaaca gaatccccgg ctcggcatga tcgta
//
LOCUS       DAOQDO010000015         8530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4484_length_8530_cov_3.632094, whole genome shotgun sequence.
ACCESSION   DAOQDO010000015 DAOQDO010000000
VERSION     DAOQDO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4484_length_8530_cov_3.632094"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..867)
                     /locus_tag="PKZ53_00570"
     CDS             complement(52..867)
                     /locus_tag="PKZ53_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ38954.1"
                     /translation="MNFIGKVGLAIVLGLGLVAILSPLFIPEDRAISQNLEQSFAPPS
                     VEHPMGLDENGRDVLARVVYGTRISLKVGIIVVAISSVVGTIIGMIAGYYGGWVDQLI
                     SGFVFNIFLAFPGILLAIAMVAFLGASLNNLILALCVIGWVGYARLIRGQVLKVREYD
                     FVTAARALGASDARIIFKHILPNSIQPLIVQASLGMAGAILSEASLSFLGLGVPPPTP
                     SWGAMLNDARLHFASGIHLTLFPGIMVTLSVLGFNFIGDGLREWLDPKQKERG"
     gene            complement(949..2715)
                     /locus_tag="PKZ53_00575"
     CDS             complement(949..2715)
                     /locus_tag="PKZ53_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ38955.1"
                     /translation="MGSPLIVLTASASEASEFKHSIWQQMLSATIPQKFSKTFINPDS
                     LINESWPDGRAKYVPNGLRVVETLLLRNYSEDDVVTCYVEHLEKFVGPETRVLAIHAH
                     NPLGISYATDVYSKLGGPNLMPLNAAEFLKIVTHPVIQKYKPKIVIGGPGAWQIETAN
                     RLDEFQVDYLITGEVERVFGDIFQRIIAGDPTLPRIINVPKNMQPTVDEIPVVRHRST
                     FGVVEITRGCGRGCQFCGPATKVGRSFPMDHIMASVNANVSEGATEIMLTSEDMFLYE
                     QLPNFVTNVPALENLFRSVKAVPGVETLQLTHITMAPVVKDPTVIERLSPLCVPYSHL
                     KHHESTDPNKCIVDPIIGLETGSPRLFDKYMKGKAYPYKAHQWRDVILKGMEILNRNN
                     WFPFCTFIIGLPGETAEDTKASLDLLYDLRDAKGMFVPTWFVPLEDSRMKGKESAKLI
                     EMSDLQWEFFFTCWKYNIEFYRGGTFERYKYSMGVPLYYKMLGKKLFGEGIKYPLYRF
                     AGFPERMLRKHLHLDFSNKKRDPKGLKPFEAEIVPAYESARNLGLMDASQAQPAPVPL
                     KKANSGLPVLSENLFQASNAGD"
     gene            complement(3244..3396)
                     /locus_tag="PKZ53_00580"
     CDS             complement(3244..3396)
                     /locus_tag="PKZ53_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38956.1"
                     /translation="MRLQALTASKIPVWKLFPVEEHLPLIGYLELLLTVPLIASMTRK
                     PSLKTG"
     gene            3505..4041
                     /locus_tag="PKZ53_00585"
     CDS             3505..4041
                     /locus_tag="PKZ53_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNJ38957.1"
                     /translation="MGFKVGDKVIYPNHGIGVVEAINQRVFDGSQLEFYQVRLHENNV
                     VVNVAVDKADEIGIRGLIKTNEGEKLLKLLSANFTTPPSDWKDRFKEFSEKMRTGDIF
                     SVAEILKHLTYLSQSKPLSFREKRMLERARFLVVTELAMVSNKAEDKVSEVVETALKK
                     ACLKHEEEAAARVATAAV"
     gene            4080..5441
                     /locus_tag="PKZ53_00590"
     CDS             4080..5441
                     /locus_tag="PKZ53_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNJ38958.1"
                     /translation="MSDSLFITGLKFLSIGGLVLANAFFVAAEFSLVAVRRSRIETLA
                     DGGNRHARTTLEVLNNLDAVISSTQLGITLASLGLGWLGESTLAHMFEPLFQMALTSA
                     MTARLSAHAFAAALSFIIITFLHIVLGELAPKSIALERTERVALVVSAPLQLFYRVFR
                     PFIWLLNACGVIVLKLFGMHGAGGHQSVYTEEEIRQLVSLSHQSGHLNADERELIHNI
                     FHFTSSVVREIMVPRQSIANIEIGATRDQVLKSLEESGYSRLPVYEKRPDNIIGVVHS
                     KDLLPILLTGKVLELASVMRKPVFIPDTAHLSEAMRQMRISKTQLAIVVDEHGSVAGI
                     ITLEDLLEEIVGDIQDEHDVEGEEAVFWPQPDGTVLIDGGISIREANRKLDLHLPESD
                     DYTTLAGFLMTEAGKLLDKQDTVLFQGATFVVEEIEGRRISRVRLHQPPTQPENSLAP
                     MGN"
     gene            complement(5472..6587)
                     /locus_tag="PKZ53_00595"
     CDS             complement(5472..6587)
                     /locus_tag="PKZ53_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ38959.1"
                     /translation="MMQWTFRKSPIQFGQSTVVRWLLWSGLCCVLVVSVALMPGLPWA
                     THCQYVLKKGMTRTRLQLARWSGLNPQLSGLYGKVNQPGVVIEVLDSPSGWASLTNQA
                     GEFQLPEEWCYPGATIDLLVTAPNRPPQVCSVAVPDNRTGSGFHLGPISLQTASTISE
                     SDILGRNSISQLKYDVENAGYYRRLFEKLTEGKASSAEKIDALNIFVAAKLNFDESTA
                     EFSSPRQMLERGSKFCGPLAIAMATLAQAGGYPARVLHLRNAGANSNFHAVVEVYYDQ
                     AWHLYDATYGIKFLNDAGTVASYEQLRHQSHLITPEIVKALIPKARRSAFAWTPDPSN
                     SLYVFLVEPREFEGIETWMPDIYASGMHHLFYFDQQV"
     gene            complement(6757..8244)
                     /locus_tag="PKZ53_00600"
     CDS             complement(6757..8244)
                     /locus_tag="PKZ53_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38960.1"
                     /translation="MRAHIPVYKISLSEQRWIGLWGCTLAVLMAGMWRYFTDASFWLD
                     EAFVASSLRLPSVETLFSRLEYGQQFPRLYLACIAALREVGGFQTHILRLLPFLCFLA
                     ATLLWARLLALRSARSNFLNVLSGFLLLGATFWLEQSAQLKQYTFDVLLGLLPFWMDD
                     EFWGAGLRNGKKKLWLVGFGLLCASSYTYPLCLAARVGGWYLVSARRSTWKLDPTALA
                     GLVLSSLGGWWWIWSTDYSFARHDLQAVTAYWSQSILQVQLAEGAIPTIKLLSRFFWG
                     WYSTKPVILAGLIPLQICGVYWVVRQWIVPKTDPDPGWGTRSCGSLMVLLGLVAASLW
                     FQYPLSPGRLTLFTLIHIQIFALEGVTCVQHLVAKAHIRDGFLHVFLFLMAVATTLAF
                     ARFVISEPPENLRPMLATIRQTPIDTVWVHPCSVAQVQTLPEGLPVRNVLFGSEEIPR
                     HQRILILWTHTGGGCDRQLDALRMQSVFWKDLHTGRNRGLAIAEF"
BASE COUNT         2197 a         2225 c         2058 g         2050 t
ORIGIN      
        1 cttcattctt cattcttcat tcttcattct tcattctcaa ttcttcattc ttcaacctcg
       61 ttccttttgc ttgggatcaa gccattctcg caagccgtca ccaataaagt taaatccgag
      121 taccgaaagc gtgaccataa tccctggaaa aagtgtcagg tggattccag aagcaaaatg
      181 cagccgggca tcattgagca tcgcacccca gcttggggtt ggtggtggca cccccaatcc
      241 caaaaagctc aagctggctt ctgacaaaat cgccccagcc attccaaggc tcgcctgaac
      301 aatcagcggt tggatcgaat taggcaaaat gtgtttaaaa ataatacggg catcactggc
      361 gcccagcgcc ctggcggcgg tgacaaagtc atattcgcgg accttgagta cctgtccgcg
      421 aataagccgg gcatacccta cccatccaat cacgcataag gcaagaatca ggttattgag
      481 actggcgccg agaaacgcca ccattgcaat ggccagcagt atgcctggaa atgccagaaa
      541 gatattgaaa acaaatccag agataagctg gtcaacccat ccgccataat aaccagcaat
      601 catgccaatg attgtgccca ccaccgatga aatcgcgacc acaatgatgc caactttcag
      661 cgagatgcgg gtcccataga ccacccgagc caggacatcg cgcccatttt catcgagtcc
      721 catcggatgc tccaccgatg gaggcgcaaa gctctgctcc agattttgac tgatagcgcg
      781 atcctctgga atgaagagtg gagacaggat cgccaccagc cccagcccca gcacaattgc
      841 aagtccaact tttccaatga agttcatgcg agtttttcga gcgaaacaac aaaggcgcac
      901 ctcacgtgcg cccttgtcaa ctcagatcaa gccgccagtg gttgagattc aatctccggc
      961 gtttgaagcc tgaaagaggt tttcagaaag aaccggcaat cctgagttgg ctttcttcag
     1021 gggaaccgga gcgggttgtg cctgcgacgc atccatcaat cccagattac gcgcactttc
     1081 ataggcgggg acgatctcag cttcaaaggg tttcaatcct ttggggtctc gcttcttgtt
     1141 tgaaaagtcg agatggagat gcttgcgcaa cattcgttct gggaaaccgg caaaccggta
     1201 gagtggatat ttgattcctt ccccaaacag tttcttgccc agcattttgt aatagagtgg
     1261 aacgcccatc gaatacttgt agcgttcaaa cgttcccccg cgatagaact caatgttgta
     1321 tttccagcag gtaaagaaga attcccactg taaatcactc atctcgatta atttggcgct
     1381 ttctttcccc ttcatgcgag agtcttcaag cggcacaaac caggttggaa caaacattcc
     1441 ctttgcgtcc cgcagatcat agagcaaatc gagtgatgcc ttggtgtctt cagccgtttc
     1501 acctggcagc ccgatgataa acgtacagaa cggaaaccag ttgttacggt taagaatttc
     1561 catccctttc aaaataacgt cgcgccactg gtgggcttta tacggatagg cttttccttt
     1621 catgtattta tcaaacaatc gaggcgagcc ggtttcaagc ccgatgattg ggtccacgat
     1681 gcacttgttt gggtcggttg attcgtggtg tttgagatgc gagtacggga cacacagtgg
     1741 agacaggcgc tcaatcacag ttgggtcttt caccactggc gccatcgtga tgtgggtcaa
     1801 ttgcagcgtt tcaactccgg ggacagcttt gacgctgcgg aacagatttt ccagtgccgg
     1861 gacattggtc acaaagtttg gcaactgctc atagaggaac atatcctctg aggtcagcat
     1921 aatttcggtg gcgccttcgc tgacgttggc attgacgctg gccatgatgt gatccatcgg
     1981 gaacgaccgt ccgaccttgg tggccggccc gcaaaactga catccacgac cgcagccccg
     2041 agtaatttcc accacgccaa aggttgagcg gtgacgaacc accggaattt catccactgt
     2101 cggctgcatg ttctttggaa cattgataat gcgtggcagc gtcggatcac cagcaataat
     2161 gcgttgaaaa atgtcgccga aaactcgttc gacttcgcca gtaattaaat aatcgacctg
     2221 aaattcatct aaccggttcg ccgtctcaat ctgccaggcg ccagggccac caatcacaat
     2281 tttcggtttg tatttttgaa taactggatg ggtaacgatt ttgaggaatt cagccgcgtt
     2341 gagcggcatc agatttggtc cgcccagctt tgagtacaca tctgtggcat aggaaatacc
     2401 cagcggatta tgggcgtgga tggcaagcac cctggtctct ggcccgacaa atttctcgag
     2461 gtgttcaaca tagcaggtga ccacatcatc ttctgaatag ttgcgtaaga gcaaagtttc
     2521 caccacccgg aggccattgg ggacatattt tgcacgccca tcaggccacg attcattgat
     2581 cagagagtca gggttaatga aggttttgct gaacttttga gggatcgtcg ctgacaacat
     2641 ttgttgccaa atgctgtgct tgaattcgct cgcctcgctc gctgaagcgg tgagaacaat
     2701 taatggtgaa cccacagagg tcactcctca cttttagtta aagatgtacg atatatcaat
     2761 ggcacgcaag cacgggtctg aacgatgacc acctggctgc ggccagttgc ttctgtctgg
     2821 tttgggtacc ggagcggaaa ggtagagaag cagccctcca cttttaccca ctgggtgaag
     2881 ctctgtcaat acgaaaattc tcacccctga agcccacacc ggttttcgac cgtggtccac
     2941 cctgtgaaaa agcctgatgc ggcttcactt ttcttaccgt tgaccaatgc cactcagaaa
     3001 aggtaaactg cctactggct ttttgtaact ttttacgtgc agttttactg tactcatgaa
     3061 agccaaacat cttcaagcca gcatcctttc gtcgtgccag tccgagtttc atgatctacc
     3121 cagccaacat caaggtcttc gcatttttcc gtagggcttt ctctttttca tgctctcgcc
     3181 ctaagaggca tgcctggagt aaatccagca atgggttttt ctctcaggat gtatatttcg
     3241 caactaacct gtttttaacg aaggcttacg ggtcatggac gcaatcaatg gaacggtcag
     3301 gagtaactcc agatagccta tcagcggcaa gtgttcctca accgggaaca gtttccagac
     3361 cggtattttg gacgccgtca gtgcttgaag acgcacaact atttcttgaa cacctggctt
     3421 ttgaccagag tgctattctt ttcggtattt gaggaatctc gacgccttcg acacgtcgag
     3481 atgtatcaag aaaggtaggt aagtgtggga ttcaaagtcg gagacaaagt gatttacccc
     3541 aaccatggaa tcggtgtggt tgaagccatt aaccagcggg tctttgatgg ctcccagctt
     3601 gagttttatc aagtgcggct gcacgaaaat aacgtggtcg tgaatgtggc tgtggacaaa
     3661 gcagatgaaa tcggaattcg ggggttgatc aaaaccaatg aaggtgaaaa gttactgaaa
     3721 ttgctgtcag ccaatttcac cactccaccg tctgactgga aggatcggtt taaggaattc
     3781 tcagaaaaaa tgcgaaccgg tgacatcttt agtgtcgccg aaatcctcaa gcatctcaca
     3841 tatctgagcc aatcaaagcc cttgtctttc cgcgaaaagc gcatgctgga gcgggcccgg
     3901 tttctcgtcg taaccgaact ggcaatggtt tccaataaag ccgaggacaa agtctctgaa
     3961 gtcgtcgaaa cggcactcaa gaaggcctgc ctcaagcatg aagaagaagc ggcggctcgg
     4021 gtggcaaccg ctgcagtcta gggcatcaca caatccggta tgtattgccc cttttgtcaa
     4081 tgagtgattc tctttttata actggtctga aatttctgag cattggcggg ctcgtgcttg
     4141 ccaatgcttt ctttgttgcg gctgaatttt cactggtcgc tgtgcgccgg tcacggattg
     4201 aaacactggc tgacggcggc aaccgccacg cccgcaccac ccttgaggtg ctcaataacc
     4261 tggatgcggt gatctcgtca actcagcttg ggatcacact ggccagtctt gggcttggct
     4321 ggctcggcga aagcaccctg gcccacatgt ttgaaccact gtttcaaatg gcgttgacct
     4381 cagctatgac cgcccggctc tcggctcatg cttttgcggc tgccctgtcc tttatcatta
     4441 ttaccttcct gcatattgtg cttggcgaac ttgcccccaa gtcaattgcc ctggaacgca
     4501 cagaacgggt tgccctggtt gtctcagccc cgctccagct tttctatcgt gtttttcgac
     4561 cgtttatctg gctgctcaat gcctgtggcg tgattgtcct gaaattattt ggcatgcacg
     4621 gtgccggtgg acatcaatca gtctataccg aagaagaaat ccgccaactg gtttcgctca
     4681 gccatcaaag cggtcacctg aatgccgacg aacgcgaact catccacaat atctttcatt
     4741 tcaccagttc tgtcgtgcgt gaaatcatgg tcccccgcca atccattgcc aacatcgaaa
     4801 ttggcgctac gcgggatcag gtgctcaaat ctctggaaga atccgggtat tcacgccttc
     4861 cggtctatga aaagcgaccg gataacatca ttggtgtggt ccatagcaag gacttgctcc
     4921 cgattttact caccggaaaa gttctcgaac tggcttcggt gatgcgcaaa ccggtgttca
     4981 ttcccgacac ggcccatctg agcgaagcta tgcgccagat gcggatctcc aaaacccagc
     5041 tcgccattgt ggttgatgaa cacgggagcg tggctggaat tatcacactc gaagatttac
     5101 ttgaagaaat cgttggtgat atccaggatg agcacgatgt agaaggcgaa gaagccgtct
     5161 tctggcctca acctgacggc acggtgttga ttgatggggg gatctcgatc cgggaagcca
     5221 accgcaaact tgatctgcat ttacccgaat ctgatgacta tacaacactg gccggctttc
     5281 tgatgactga agcggggaaa ctgcttgata aacaggatac cgttctcttt cagggcgcca
     5341 cctttgtcgt cgaagaaatc gaaggccgcc gcatttcacg cgttcgattg catcaacccc
     5401 caactcaacc tgaaaactct ctggcaccaa tggggaattg acctgatccc gatccatttt
     5461 tgtcctggcg atcatacctg ctggtcaaag tagaaaagat gatgcattcc agaagcataa
     5521 atatctggca tccaggtttc aattccttca aattcacgag gttccaccag aaatacatac
     5581 agtgaattgg agggatcagg ggtccaggca aacgcactcc ggcgggcttt gggaatgagc
     5641 gctttgacaa tttccggagt gatcaaatga gactgatggc ggagttgttc atagctggca
     5701 actgtacctg catcattcaa aaacttgatt ccataggtgg catcgtacag gtgccacgcc
     5761 tggtcataat acacttcaac caccgcatga aaatttgagt tggcgccggc gttccgcagg
     5821 tggagcaccc gcgctggata gccacccgcc tgggccaggg ttgccatggc aatggccagc
     5881 ggcccacaaa actttgaacc tcgttcaagc atctgccggg gcgaagaaaa ttctgccgta
     5941 gactcatcaa aatttaattt cgcagccaca aaaatattga gggcatcaat tttttcggcg
     6001 gatgacgctt ttccttcggt caatttctca aacaaccgcc ggtaataccc ggcattttcc
     6061 acatcatatt tgagctggct gatagaattg cgacctaaaa tgtcagattc actgatcgtg
     6121 ctggctgttt gcagggaaat cggtcccagg tgaaatccag atccagtccg gttgtcaggt
     6181 acggcgacgg aacacacctg cggtgggcga tttggggccg ttaccagtaa atcaatcgtc
     6241 gcccctgggt aacaccattc ttcaggtaac tgaaattccc ctgcctgatt ggttaaactg
     6301 gcccatccag aaggagaatc aaggacctca atgactactc ctggttggtt tacttttcca
     6361 tagagccctg atagttgagg atttaagccg ctccatctgg caagttgaag gcgggtccga
     6421 gtcatccctt tctttaagac atactgacag tgggttgccc agggcaatcc gggcatcagc
     6481 gccacactca ccaccagcac acaacacaaa ccactccaga gcagccacct gaccaccgtt
     6541 gactgcccaa actggattgg agattttcga aaagtccact gcatcattct ctcaccttgt
     6601 caggaaacgg gtggtccatc gtcagtcgtt gagtgttggg ggaaagagaa ggttcaaatg
     6661 tattgtgttc tatttggaca gagtacagat gtcttttaag aacacacaac caaaaacccc
     6721 tgatcgtatc aactgactcc tgacgatgac ctcatcttaa aattccgcaa tcgccagacc
     6781 gcgattgcgt ccggtgtgaa gatccttcca aaacacagat tgcattcgca gggcatcaag
     6841 ttgtctgtcg catccaccac cagtgtgtgt ccagagaatc aaaattcgtt ggtggcgagg
     6901 aatttcttca cttccaaaga gcacatttcg taccggtaac ccttcaggca gcgtttgaac
     6961 ctgagcgaca gagcagggat gaacccagac tgtatcaatc ggtgtctggc gaatagttgc
     7021 cagcattggc cgcaggttct caggtggttc actgatgaca aacctggcaa atgccagcgt
     7081 cgtcgccact gccatcaaga acaaaaacac gtgcaggaat ccatcacgaa tatgcgcttt
     7141 tgcaacaagg tgttgaacgc acgtcacacc ttcaagtgcg aaaatttgaa tatgaatcag
     7201 tgtaaacaag gtcagtcgcc caggactcag tgggtactga aaccacaaac tcgcggcaac
     7261 gagtccgagc aaaaccatca aactgccaca ggatcgggtt ccccaccctg gatccgggtc
     7321 agttttgggt acaatccact gacgcaccac ccagtagaca ccgcaaatct gtaatgggat
     7381 caaccctgcc agaatcacgg gtttggtact ataccagccc cagaaaaacc gactcaggag
     7441 tttgatggtt gggatggcgc cttcggccag ttgaacctgc agaattgact ggctccaata
     7501 cgccgtgacc gcctggaggt cgtggcgagc gaaagaataa tccgtactcc agatccacca
     7561 ccatccaccc aaacttgaaa gcaccagtcc tgccagagcc gtagggtcaa gcttccaggt
     7621 tgatctgcgg gcactcacca gataccaccc gcccactcgt gctgccagac ataacgggta
     7681 cgtgtatgag ctggcacaca agagtccaaa cccaaccagc caaagctttt tctttccgtt
     7741 tcgcaacccg gctccccaaa attcatcatc catccaaaac ggcaacagtc cgagcaaaac
     7801 atcaaacgtg tactgtttga gttgggccga ttgctcaagc cagaaggtgg ccccaagcag
     7861 cagaaacccg ctcaaaacat tcaagaaatt cgatctggcc gaacgcaacg ccagtaatcg
     7921 cgcccacaac agggtggctg ccagaaaaca gagaaatggt aataaccgca aaatgtgggt
     7981 ttgaaatccg ccgacttcac gcagtgctgc gatacacgct aaataaaggc gtgggaattg
     8041 ctgaccatat tcaagtcgtg aaaaaagggt ttcaaccgat ggtaatcgga gggatgaggc
     8101 gacaaacgct tcatcaagcc aaaaagaggc atctgtaaaa tagcgccaca tcccggccat
     8161 caagactgcc agcgtgcacc cccatagccc aatccacctc tgttcagaga gactaatttt
     8221 atagacagga atatgggctc tcatgcggag tatcggcctc gcttttcgaa aatttgattg
     8281 attgaaagag aaaaaaagcc cagtttggtg agaactgggc ttgaaagaca caacaacaag
     8341 actcgggaga gagcttgcaa cgtcgagtct caatcgcact tgtcagtttg tggtgaacct
     8401 tcctggtgca aaacggacta tagccggttt tctcatgcga gttgttaaga ggcggaaaag
     8461 attttgttaa ggatgtaaaa tcttctccct cacaggtttc caggttttat gcctgtgata
     8521 gtatcaggcc
//
LOCUS       DAOQDO010000016         8329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4683_length_8329_cov_3.807469, whole genome shotgun sequence.
ACCESSION   DAOQDO010000016 DAOQDO010000000
VERSION     DAOQDO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_8329_cov_3.807469"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKZ53_00605"
     CDS             <1..708
                     /locus_tag="PKZ53_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNJ38961.1"
                     /translation="KPHKKGEVNPSAQPGDDRPITGEDYQRLGAVVIFCFFAAMFWAV
                     FEQAGSSLTLFADKMTHNVILGVSFPSSFYQSVNPSFIVLLTPVFAWLWIKWGDKQPS
                     TPMKFSIGLLFAGLGFLVAALATNVAGGGQVSAWWLIGVYFIHTVGELCLSPVGLSTM
                     TKLAHPRLVGMVMGFWFLGPWAGNLAGGLIAGHFDSSDASAVIGLFLKVGMSAVVGAI
                     IMAALSPIIRKLMVGVK"
     gene            772..999
                     /locus_tag="PKZ53_00610"
     CDS             772..999
                     /locus_tag="PKZ53_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38962.1"
                     /translation="MPQPLQYLTDAQGNKVAVVLPLAEYGSLTEDLLEDLYLAKISRE
                     TEGDELIPFEQVMAELKCMDSATSGSRSVSN"
     gene            complement(1114..2607)
                     /locus_tag="PKZ53_00615"
     CDS             complement(1114..2607)
                     /locus_tag="PKZ53_00615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ38963.1"
                     /translation="MRTLNRMKKMYLLSLVLPALVVMGGFFQPNQAQYQKLPPLDPPK
                     SVTFPKVVSKTLPNQLRVYVVEDHSLPIVALQLVTEAGSVTSGTSTPGLAFFTARMLD
                     QGTQTRSAQDIAGAIDSIGGTLSASAAYESAEISSAVLTEHSQLGFELVADLVQNAAF
                     KPQEIERLREQLLGELQVEYGTPSFVADTALRRLVFGMTPAGTPLVGTPDTLKKLTRA
                     QVINFYKQNYIPNTSFLVVGGDISPDEAFARAEKAFGTWKPGKAQKSVRLAQPAMAKQ
                     LQCVVIDKPGAAQTEVRVGHPGITQIDPRYFQGLVMNSVLGDGYSARLNKEIRIKRGL
                     SYGARSNLVAGRGIGLFRTRCSTKAQSTFEVVQIFLDEMKRIQAEAIADEELATRKLA
                     TTGDFVQELQSIQAVMDQVATTIHLGQDLKTLDEFIPKVNAVTVAQAQDFAKRQLNVT
                     TPMIVLVGDYKVFEKDLTALKATPTIIKMGDFDPLQPKLAKATKAKK"
     gene            complement(2604..3989)
                     /locus_tag="PKZ53_00620"
     CDS             complement(2604..3989)
                     /locus_tag="PKZ53_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ38964.1"
                     /translation="MPKRKMLALLALVCLWLSTGLGWSPAQAQTKLPKLKIQTAELPN
                     GLKVVLHEDHRLPLVAIQVWYHVGSKDERPGRTGFAHLFEHLMFKGTKNLPSESIDRL
                     TEDVGGMNNAFTDDDVTVYLEIVPAHHLRRLLWAEADRMGALAVTEENFRSERDVVKE
                     ERRQSVENAPYGMVDEILYASVFTKHPYQHTTIGSMEDLNRATLADVNEFHQSFYAPN
                     NATLVLSGDFDSAAILPVIKEYFTPIPASSQPISRITVVEPPQTAERRLTYKDPNAPL
                     PQVRIVFHTPGNGHPDSYALRLLGAILSNGQSSRLYQSLVYQQGLATEANGGGFFSEH
                     PNLFSFVATLAPEKTLPEGEKSLLAEIEKLQTSGVTESELQKAKNQLLASKVNGWQSL
                     MERAEALGRAATFFGDPNRALTDMERLQTVTMADVQAVAKKYFTTENRTVIYVSLPEA
                     AEATKTAGGAK"
     gene            complement(4230..4682)
                     /locus_tag="PKZ53_00625"
     CDS             complement(4230..4682)
                     /locus_tag="PKZ53_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38965.1"
                     /translation="MRGSKRSFVSLMHLFSLALICSVLMMGVGCKSETQSSGPAGQPA
                     QPQGAQPVTPPPAPAAPNQALPAPPPPPPGGVVASPTGLPAKEADIVVKTPTEGLQKD
                     SQGRVMKTRENMEKIREARKNAPTEVRKAELPKQPTPQPDPSKQKPKQ"
     gene            complement(4735..5487)
                     /locus_tag="PKZ53_00630"
     CDS             complement(4735..5487)
                     /locus_tag="PKZ53_00630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ38966.1"
                     /translation="MFIGSSILTGVRGLAASLHCRRSLWMVIGVLALVVIGGLTLDSS
                     VVSGQQTRKKTTKTSQKKRTTRRRKPAATKPPAAKPPVPAPAPTPVAAPQVTLETNFG
                     TIRFELLTQDAPKTCENFRLLTSRGFYDGLTFHRVINGFMIQGGDPNGNGTGGESAWG
                     GEFDDEINASSPLYQTGYAQGIVAMANRGPNTNGSQFFIMHKPYPLPPNYTIFGRVLE
                     GMDVVDKIATAPTGRNDRPITAVIIHKATVQP"
     gene            complement(5494..6189)
                     /locus_tag="PKZ53_00635"
     CDS             complement(5494..6189)
                     /locus_tag="PKZ53_00635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ38967.1"
                     /translation="MSNSLKSLVRTLGVLCLCLAGFFAQGCKPEAPPAPSSPATPTAP
                     SKPESPAAPAAPSGETKPAQSEPGKKLLATLVTNKGTIEFELLTDDAPKACDNFRLLA
                     NRGYYNNLIFHRVINGFMIQGGDPNGNGTGGESAWGGTFDDEINPSSPLYQGGYKPGI
                     VAMANRGPNTNGSQFFIMHKNYPLPPRYTIFGRVTKGQNVVDSIATSPVGPNDMPLSK
                     VIITSAKVVEMGG"
     gene            complement(6390..6887)
                     /locus_tag="PKZ53_00640"
     CDS             complement(6390..6887)
                     /locus_tag="PKZ53_00640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ38968.1"
                     /translation="MSTKLIATLETSKGTIRFELLTQDAPKTTENFKLLADRGYYQGI
                     IFHRVINGFMIQGGDPTGTGAGGESVWGRTFADEINPSSAIYQTGYVPGTVAMANRGP
                     NTNGSQFFIMHKRAALPPNYTIFGRVIAGQDVVDAIATTPTDHRDRPLTPVTIQRATV
                     EEVNA"
     gene            complement(6994..8043)
                     /locus_tag="PKZ53_00645"
     CDS             complement(6994..8043)
                     /locus_tag="PKZ53_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNJ38969.1"
                     /translation="MMKKSKTSPYLKHPTQPVQIDRDRSVAGLLNKMETSSFQARNLA
                     IAHNIWLNMLDDNTTIVLGISGALVAAGMRRIIAWLIRNRYVDVVVSNGANIFHDVHE
                     TLGRQHYLGSPSLDSAELQNLAIGRVYDTLISEEEFREADQWLASFINTLEQNRPYST
                     REFLHLLGHELSEIANEDGILTSAFKARVPVFCSGVVDSVLARGIAASRIDRKSPFMF
                     DVIQDVVEMAHILAGSVNTGATFFGTGTPPNFVKQAHSTASVINSRIRDHKYFLQVIT
                     ESPYMSSSISGHLEDKENWTKPSKELRSVSVYCDPTIAMPILVTGLSQSATKQIKQRR
                     KPTFTHGRELGVTFA"
     gene            complement(8150..>8329)
                     /locus_tag="PKZ53_00650"
     CDS             complement(8150..>8329)
                     /locus_tag="PKZ53_00650"
                     /inference="COORDINATES: protein motif:HMM:NF037681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38970.1"
                     /translation="QGSNLGDASATARYERSFLHESFSRLAAEQVKAGDLSPSEADKL
                     CREYENSINYYTYLG"
BASE COUNT         1919 a         2106 c         2212 g         2092 t
ORIGIN      
        1 aaaccacaca aaaaaggtga ggtaaatcca tcggctcaac ccggtgatga tcgcccaatc
       61 acaggtgaag actatcaacg cctgggcgcc gtggtgatct tttgcttttt tgcggccatg
      121 ttctgggcgg tatttgaaca ggctggctcc agcctgactt tgtttgccga caaaatgacc
      181 cacaatgtca ttttgggcgt gagttttcca tccagcttct atcaatcagt caacccaagc
      241 tttatcgtgc tgttaacacc agtctttgcc tggctctgga tcaagtgggg tgataaacag
      301 ccatccaccc caatgaaatt cagcatcggg ctgctgtttg ctgggcttgg gttcttagtc
      361 gccgcattgg ccaccaatgt tgccggcggc ggtcaggttt cagcctggtg gttgattggc
      421 gtgtatttta ttcacacggt tggtgaactg tgcctgagcc ccgttggact gagcaccatg
      481 accaaactgg cccatccgcg cctggtcgga atggtaatgg gcttctggtt cctgggcccc
      541 tgggctggga acctggccgg aggacttatc gccgggcact ttgattcaag cgatgccagc
      601 gccgtaattg gcctgttctt gaaggtcggg atgtcagccg tggttggggc aatcattatg
      661 gcagccctct caccaatcat ccgtaaactg atggttgggg tgaagtaaaa ctgaccagcc
      721 ttgatagggt gaacaccagg tcaagagtgg actcttttct ggaggtaacg tatgccgcaa
      781 ccacttcaat atctaacaga tgcgcaaggg aacaaagtgg cagtcgtctt gcctctggct
      841 gaatatggtt cattgaccga agatcttctg gaagatttgt atcttgcaaa aatttcacgt
      901 gagactgagg gagatgaact gattccgttt gagcaagtta tggccgaact caaatgtatg
      961 gatagcgcga ctagcggttc cagaagtgta tccaactgat ctggttttgg ttctccacaa
     1021 aaaccaaagg gcggttcacc actcaggtga accgcccttt tttctttgga cttcgtgatg
     1081 aaacccaccc gctcaggcag gcagtactga cctttatttt ttagctttgg ttgctttggc
     1141 cagctttggt tggagcgggt cgaagtcgcc catcttgatg atggtcggag tcgctttcaa
     1201 agcggtcagg tctttttcaa acactttgta gtcaccaaca agcacaatca tcggggtcgt
     1261 cacattcaat tgccgtttgg cgaaatcctg cgcctgggca accgtcacgg cattcacctt
     1321 gggaataaac tcatccaggg tcttgaggtc ctgcccaagg tggatggtgg tggctacctg
     1381 atccatcacg gcctgaatgg attgcagttc ctgcacaaag tctccggtgg tggccagctt
     1441 gcgggtggcc agctcttcgt cagcaattgc ttcggcctga atgcgcttca tctcatccag
     1501 aaaaatctgg acgacttcaa aggtggactg ggctttggta ctgcaccggg tccggaataa
     1561 tccaatccct cgaccagcca ccagattgct ccgggcgcca taactcaaac cacgtttaat
     1621 gcggatttcc ttgttcaggc gagccgaata gccatcaccc aacacgctgt tcatcaccag
     1681 tccttgaaag taccggggat caatctgggt gatgcctgga tgtccaacgc ggacttcggt
     1741 ttgggcggca cctggtttat caatcaccac gcactgaagt tgtttggcca tggctggttg
     1801 cgccaatcgg actgatttct gggctttacc aggcttccag gtgccaaagg ctttttcagc
     1861 tctggcaaag gcttcgtctg gggaaatatc gccgccaacc accaggaaag aagtgttggg
     1921 gatatagttt tgcttataga aattgatcac ctgggcacgg gtcaattttt taagtgtatc
     1981 tggcgttccc accaggggcg tcccagccgg ggtcatccca aacacaaggc ggcgcaaggc
     2041 agtgtcagcc acaaacgacg gcgtgccata ttcaacctgc aattcaccca gaagctgttc
     2101 gcgaagccgt tcgatttcct ggggtttgaa cgccgcgttt tgaaccagat cagccaccag
     2161 ttcaaaccca agctggctgt gttcggtgag aacggctgat gaaatttcgg ctgattcata
     2221 ggcagcactg gctgaaaggg tgccaccgat tgaatcaatt gccccggcaa tatcctgagc
     2281 acttcgggtt tgggttccct gatcaagcat tcgggcagtg aaaaaggcca atccgggcgt
     2341 tgaggtccct gatgtgactg aaccagcttc agtgaccagt tggagggcga caatcggcag
     2401 gctgtgatct tccacgacat acacccgaag ctgattgggc agcgtttttg agacaacctt
     2461 tggaaaagtg actgatttgg gcggatccag tggcgggagc ttttgatatt gcgcctgatt
     2521 gggttgaaaa aacccaccca ttacaaccag tgctggcagt accagactga gcagatacat
     2581 ttttttcatg cgattcaagg ttctcatttg gcacctccgg cggttttggt cgcttcggca
     2641 gcctcgggta aggacacgta gatcaccgtt cggttctcgg tggtgaagta ttttttggcc
     2701 acggcctgaa catcagccat ggtcactgtt tgtaaccgct ccatatccgt cagggctcga
     2761 tttggatcgc caaaaaatgt tgcggcccgg cccaatgctt cggcgcgttc catcagggat
     2821 tgccagccat tgacctttga ggccagcaat tgattcttcg ctttttggag ttctgattca
     2881 gtcacgcctg aggtctgcag tttttcaatc tcagccagca aggatttttc accctccggt
     2941 aaagtttttt cgggcgccag cgtggccaca aaactgaaca gattggggtg ttctgaaaaa
     3001 aatccgcccc cattggcttc ggtggccaga ccttgctggt aaaccagcga ttgatagagg
     3061 cgtgagcttt gaccgtttga gagaatggca cccaacaacc tgagcgcata gctgtcgggg
     3121 tgaccatttc ctggtgtgtg aaatacaatc cgcacctgtg ggagcggggc atttgggtcc
     3181 ttataggtca accggcgctc ggctgtctgg ggcggctcaa ccaccgtaat gcgggaaatc
     3241 ggctgtgaac tggccggaat cggggtgaaa tattctttga tgacgggaag gattgccgct
     3301 gaatcaaaat ctccggaaag caccagcgtt gcattgtttg gcgcataaaa cgactggtga
     3361 aattcattga catccgccag tgtcgcccgg ttcaggtctt ccatgctgcc aatggtcgtg
     3421 tgctggtacg gatgtttggt aaacaccgag gcatacagga tttcatcaac cattccataa
     3481 ggcgcgtttt caactgactg gcgacgttct tctttgacaa cgtcacgttc ggatcggaaa
     3541 ttttcttcgg tgacagccaa cgcccccatg cggtcagctt cagcccagag caatcgtcga
     3601 aggtgatgtg ctgggacaat ttccaaatac acggtcacat catcatcagt aaaggcgttg
     3661 ttcattccgc caacatcttc cgtcaaccgg tcaatggatt cactcggcag gttttttgtt
     3721 cctttaaaca ttaaatgctc aaacaggtgg gcaaacccgg tgcgtccggg gcgctcgtcc
     3781 tttgagccaa cgtgatacca gacctggatg gcgaccagtg gtaagcggtg gtcttcgtgc
     3841 aataccactt tcagaccatt gggcaattcc gccgtttgaa tttttaattt aggcagtttg
     3901 gtttgagcct gagccgggct ccatcccaat ccggtactca accacagaca taccagcgcc
     3961 aggagcgcca gcatttttcg tttcggcata ctctgacaga cctcatctct gaaaataaaa
     4021 gtgcagacgg cacccgatgc agggtaacga ggaaaatgaa aaatgcaacc ctttcgcaga
     4081 caactgacgg atcaatctca agatcagttg cacagggctc agtcttgtga gtttttcgcg
     4141 gcgggagaaa caattgggct ggacttacag gtaagtaaat ccagcccaaa aacgacctgg
     4201 tatcagtttt gattgaaacg acggcgcact tactgttttg gcttttgttt tgaaggatcc
     4261 ggctgaggtg tgggttgttt cggtaactcg gccttccgga cttcggtcgg tgcattttta
     4321 cgcgcttctc tgatcttttc catattttct cgggtcttca tcacccgacc ctgagaatct
     4381 ttttggagcc cctcggtggg tgttttcacc acaatatcgg cttctttggc aggcagccca
     4441 gttggcgagg caaccacccc acctggcggt ggtggcggtg gagcaggcaa cgcctgatta
     4501 ggtgccgccg gtgccggtgg cggggtaaca ggttgtgccc cctggggttg agccggttgc
     4561 ccggctgggc cacttgattg ggtttcgctt ttacacccaa cgcccatcat cagtactgag
     4621 cagatcaaag ccagtgagaa caggtgcatc aaagaaacaa acgaacgttt tgaaccacgc
     4681 ataagacttt actacctcaa aaaaaaattc caatgccgtt ggccaccaaa cgacttatgg
     4741 ctgaacggtt gctttgtgaa taatcaccgc agtgatcggt cggtcgttac gaccagttgg
     4801 agcagtggca attttatcaa cgacatccat cccctcaagc acacgtccaa aaattgtgta
     4861 attgggtggg agtggatagg gtttgtgcat aatgaaaaac tggctgccgt tggtattggg
     4921 accacgattg gccatcgcca caatgccctg ggcatatcca gtttggtaca gtggggaact
     4981 ggcattgatt tcatcatcaa attccccgcc ccaggctgac tcacctccgg ttccattgcc
     5041 atttggatcg ccaccttgaa tcataaagcc attgatgact cgatgaaagg taagcccatc
     5101 ataaaatccc cggctggtca gcaatcggaa attttcacag gttttcgggg catcctgggt
     5161 gagaagctca aatcgaatcg tgccaaaatt ggtttcaagt gtcacctgcg gagccgctac
     5221 cggtgtaggt gcaggcgccg gcactggtgg tttggcggcg ggcggtttgg tggcagccgg
     5281 cttgcgccgg cgggtggtgc gctttttctg actggttttg gttgtttttt tccgcgtttg
     5341 ctggccggaa acaaccgatg aatccaacgt gagaccacca atgacgacca gtgccagcac
     5401 cccgattacc atccagagtg atcgtctgca gtgcaggctg gccgccagcc ccctcacgcc
     5461 tgtcaggatt gaacttccaa taaacatcac ggtttagcct cccatttcaa caactttggc
     5521 actcgtgatg atcacttttg acagaggcat atcatttgga cccactggcg aggtggcaat
     5581 cgagtccacc acattttgcc ccttggtcac gcggccaaaa attgtgtacc tcggaggcag
     5641 cgggtagttt ttatgcataa tgaaaaactg gctgccgttg gtgtttggac cacgattggc
     5701 catggctaca ataccgggct tatacccgcc ctggtagagg ggtgaactgg ggttgatttc
     5761 atcatcaaag gttccacccc aggctgattc cccgccagtc ccgtttccat tgggatctcc
     5821 gccctggatc atgaacccgt tgatcacccg gtgaaagatc aaattattgt aatagccccg
     5881 gttagccagt aatcggaagt tgtcacaggc tttcggagca tcatcggtta acaattcaaa
     5941 ctcaatcgtg cctttattgg ttaccagagt tgccagcagt ttttttcctg gctcactctg
     6001 ggcaggtttt gtctcgcccg atggggcagc aggggccgcg ggggattcag gcttggacgg
     6061 cgccgtgggc gtcgccggtg atgaaggagc tggcggagct tcgggtttgc acccctgggc
     6121 gaaaaaacca gccagacata gacacaacac accaagggtc cgaacaaggg atttcagtga
     6181 attcgacata gatttttgga tgagtcagtt gagctgggga acgcctttgt gtctgccttg
     6241 atgtcgcacg accagcaaaa tggttcccag tttggaaaaa tttcaggtta aaacaccaaa
     6301 cccgctctgg caaaggattt gccggcgggc ttgtcaaaag gcgaacactc aagaattcct
     6361 gaatagacaa aagtttattc gccggcagat caggcgttga cttcttccac ggtggcccgc
     6421 tggattgtaa ctggcgtcaa tggccggtca cggtgatcgg ttggcgtggt tgcaatcgca
     6481 tcaaccacat cttgaccggc aatcacccgg ccaaaaatgg tgtagtttgg tggaagcgcc
     6541 gcccgtttgt gcatgatgaa gaactgactg ccgttggtgt tgggtccccg attggccatc
     6601 gcaacggttc ctggtacata cccggtttga tagatggccg aggagggatt gatttcatcg
     6661 gcaaaggtcc tcccccagac cgattcccca cccgcgccag tccccgtcgg atcaccaccc
     6721 tggatcataa atccattgat cacccggtga aaaataattc cctgatagta cccgcgatca
     6781 gccagtaact tgaagttttc agttgttttt ggcgcatcct gggttaacag ttcaaagcga
     6841 atcgtgcctt tgctcgtctc aagtgtggca atcaattttg tagacatgtt gtttttccgt
     6901 tcaaaattca gggtttaggg tttagggttt tcgaggaaat accttttggt tcttttggtt
     6961 cctgtggcgc caccagaaag gcagtaccaa aaatcaggca aacgtaaccc caagttcacg
     7021 cccatgggta aaggttggct tccgacgctg tttgatttgc ttggtcgcgc tttgggaaag
     7081 tccggtcact aaaattggca tggcaatcgt cgggtcacaa taaaccgaga cacttcgcaa
     7141 ttccttggac ggcttggtcc aattttcctt gtcttccaga tggccggata tactgctgct
     7201 catatagggt gattctgtaa tcacctgcaa aaagtatttg tggtcacgaa tccgggaatt
     7261 gatcaccgaa gcggttgaat gagcctgttt gacaaagttt ggcggtgtcc cagtcccaaa
     7321 gaaagtagca cccgtgttga ccgaaccggc cagaatatgg gccatttcga ccacatcctg
     7381 aatcacatca aacataaagg gtgatttgcg atcaattcgg ctggccgcaa tgcctcgggc
     7441 caacactgaa tcaaccaccc cggaacaaaa caccgggaca cgggctttaa acgccgaggt
     7501 cagaattcca tcttcattgg caatttcaga caactcatgg ccaagtaaat gcagaaattc
     7561 acgggtggaa taggggcgat tttgttcgag tgtattgata aaggacgcca gccactgatc
     7621 agcttcacgg aattcctctt cgctaatcag cgtgtcatag acgcgaccaa tcgccagatt
     7681 ctgtaactcg gcgctatcca ggctggggga acccaaatag tgctgccgtc caagcgtttc
     7741 atggacatcg tgaaagatgt tggcaccatt tgaaaccacc acatcaacat accggttgcg
     7801 aatcaaccag gcgatgatcc gtcgcatccc tgctgccacc agcgctccgg aaatccccaa
     7861 cacaatggtg gtgttgtcat ccaacatgtt gagccagata ttatgcgcaa tagccagatt
     7921 gcgggcctga aacgaagagg tttccatctt gttgagcagg ccagcgacac tccggtcacg
     7981 atcaatctgg acgggttggg tcgggtgctt gagatacggg gaggtttttg attttttcat
     8041 catacgccgg ttggaatcag gatcagagaa agccggaaac gggaaaagtc aggcaggaag
     8101 tcgtcatgtt tcccattttt ctggctgagg ggttggcagc agcaaccagc tatccaagat
     8161 aggtatagta gttgatgctg ttttcatact cacgacataa cttgtcagcc tcagatggag
     8221 agaggtctcc agctttgact tgctcagccg ccagacgcga aaagctttcg tggaggaaag
     8281 aacgctcata gcgagcggta gctgaggcat ctccaagatt gctcccctg
//
LOCUS       DAOQDO010000017         8223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_4791_length_8223_cov_5.466699, whole genome shotgun sequence.
ACCESSION   DAOQDO010000017 DAOQDO010000000
VERSION     DAOQDO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_8223_cov_5.466699"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2004)
                     /gene="asnB"
                     /locus_tag="PKZ53_00655"
     CDS             complement(88..2004)
                     /gene="asnB"
                     /locus_tag="PKZ53_00655"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ38971.1"
                     /translation="MCGITGAIGKVTEADLIRMLDAIQHRGPDDRGTYVKPTGTGETV
                     ALGHQRLAILDTSPAGHNPMPNATRRRWMIYNGEVYNFREIRRDLEALGHRFKSESDT
                     EVILAAYDEWGVDHLQRLNGMFAYVIWDETTDTLFAARDRLGIKPFYYTTHHGGFAFG
                     SEIKSLLQVPGVKAELDLEAFNDYLTFLWVPDPKTTFRNIFKLPPGHFLMWQRGKLTV
                     QPYWDVTFAEDDSLSLKSWEATVREELTLSVERNLVSDVPLGTFLSGGVDSSSIVGLM
                     TRMFETQTPRRKVQTYTVGSTPEDLAYDVNEDDTAYARQVGKLFETDYHESIIRPDVI
                     ELLPKMVWHMDEPVADPAILTSYLICRAARETLTVLLSGMGADEIFAGYPRHLAAVRL
                     AKMYNLVPQAISQPLVAMLPGAGAGTLSKLSRNVKKLAKSAALPFQERYLGYGTYFTD
                     AEKQALYSPELRATASTFDAYRMHRTYLERVKDQHPVNQMIYLDLKTFLPCLNLTYSD
                     KTSMAASVEVRVPFLDHMLVELSARVPAEYKLHGTTRKFILKRCLEDLLPKQILYRKK
                     AGFSAPVRAWLLRDLREMVDDLLSAETLKKRGYFDPVEVQRLIQQHRAGREDNALKIY
                     QLLTFELWCRKFMD"
     gene            complement(2158..>4583)
                     /locus_tag="PKZ53_00660"
     CDS             complement(2158..>4583)
                     /locus_tag="PKZ53_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38972.1"
                     /translation="HSFPGFFMKFYRFFFIFTVIVSLATGINQAVPQLEVMTAQAHPE
                     TTGGSDAAFSASNQTSQINTLAFPRAFFPGVNNNLVVSDDGTFGLVGSYETDSLFTFD
                     MTTGEQLGEVTVGDGPTQVIVQGTLAAVFNNAFLSQPQPTISIVDVSNPRVPHLVSTC
                     LFPEKRSAYFTQETLSLSRDGQVVFGTVVDKKGRGTLFAFDTQTGEQLGTLALGDFPV
                     QLALREKDGKRLLAITSTPDGPHNLFLVDATNPRALRQQAVYRFPDRGALSLSNTVLL
                     SDSEDFGYIAIARGQLLAFSTTTAEILSRTTLPIFPARLQRRGNRMLVQHDSEALITV
                     LDLTEPLAPKQVSQVEFTAEQLLYSPPRFSPDGTRGYALVPFEQRVEAFDIETGERLG
                     SSKVSEEIPFAFAVGTTSENPVCAVMTTSERRVDLVRSLPNGFEPVGNFETPTGVAFT
                     IKQVPVVTADGTKAYIASPLTDEVFEISLETNAVTARLTTDDNPETLSIYENATERRL
                     AVTTRGGSSVNLFRLNQTGDWETTGSVNINTSNPFLLDYSNVVFSPDGKTGFVTDVET
                     RVYAFETDGGAVLSSLEVGPFPAQLGFVQTLEGNQLLAVTAGSLGEDSTITVLNVNDP
                     RAMQPVSTTRVDKEIQLALDNRPLFSTDGKQLYLGASFSGELLVIDVAKEQIVNQIKG
                     ITGVVLNPFEKNGKPAFITANLDDGKILILERDQTQGLRKSQRIKPPQNNYFIVSNLP
                     VIFADNENGAIASYGLQSLLFFNINQGQTLNQIQLPGAPGRVALVPGSQTLLTVEING
                     SANRIFRID"
     assembly_gap    4584..4683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4946..6253)
                     /locus_tag="PKZ53_00665"
     CDS             complement(4946..6253)
                     /locus_tag="PKZ53_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ38973.1"
                     /translation="MYSADPSPAFIDFPAVDTLSTEIKFQLIQEITHKISTSLDIDEI
                     LNLIIDAVRSFVRYDAAAIYIVESKSNEKRMQALVHRAYDVSRTNMLRLKFGEGLVGW
                     VIKNSKGVIIPDVRNDSRYIEADPETKSEIVAPIISNGKVIGAFNLESHEKTTYTERD
                     LDMLMFFAGTAAISIEKAFLHQERLEKKRLESELGIARQVQLSLLPDRDPVIPGFDIS
                     GANFPTEEVSGDSFDFISFPNQQLGICIADVSGKGIPAALIMASFRASLRAQVRNDYA
                     IRSIFRKVNYLLWESVTGAQYVTAIYGVLDPFSHRFTYADAGHNPMILLRADNTHLEL
                     SCGNTVLGMFEDIDYRECNRRLKPGEIVILYTDGVTEAARDGVELGPQGLINIVKELR
                     HLPAREMVHQIYEAVRKYAQQSVLDDDLTVVIIKVLEEQKNEE"
     gene            complement(6415..8007)
                     /locus_tag="PKZ53_00670"
     CDS             complement(6415..8007)
                     /locus_tag="PKZ53_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38974.1"
                     /translation="MAAEMAEARQLAAGLVVRQWFLAGNRRRAVTLARRTGAFADAIV
                     RLEKTDPQQANTLRLLWATTQAEAGEYPAAVNTIWPVSEARHIAADWIECAIAGGGVA
                     AGQMLAYKLTLFPTDHEAMSGPILDLLAEEDAEKAPLRAAFRRTLVADAPKGPVPQML
                     ARAAVRATIRDMSQGHIQTTTNELTNLLTFASDAALRTDVPTVTSPHLPKLQTSPKPR
                     IFTADATDTGLMPVYDAAYLPDGKCLVAIGESGIRLLSNQGKTLTHFDKPATALVLSD
                     QGNRAIGLIRRGEVWKLTKLDLTTRSASDWCEAELSDFARDFDGQTWVVNSRDDILVI
                     DTLGKRFEALWRIPEVGRTLTISRSTSQCSLFVVNGQHKQVEIWTYALPQFRLQSREA
                     ITFTPDPVLERTVALNPGGFSLNAALQTDTHLVQVTRCWQKSFLSKQELTTATALSPN
                     QPVLTTNWMVIPVRTENEVRVYVLDWAHTQTRLELLLNATREISLRLTNDDTLTICDH
                     RGRIFVFELTYGNQLRNLRMGM"
     gene            8020..>8223
                     /locus_tag="PKZ53_00675"
     CDS             8020..>8223
                     /locus_tag="PKZ53_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008029542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNJ38975.1"
                     /translation="MAFQKGNGFFVFLQEFGQVRAVKETPGLGVKIPFIQNVVFYDSR
                     IQGIDTPSLEVTPLDDRRLIVDAF"
BASE COUNT         1917 a         2079 c         2197 g         1930 t
ORIGIN      
        1 ggttccgggt tccgggttcc gggttccggg ttccgggttc cgggttccgg gtttcaatgt
       61 attgtcactt tgtcattaga tcaaaattca atccataaat ttccgacacc atagctcaaa
      121 ggtcaagagt tggtagatct tcaaggcgtt atcttcgcgt ccggcgcggt gttgctggat
      181 cagtcgctgg acttccactg gatcaaaata cccgcgtttt ttcagtgttt ccgccgaaag
      241 caggtcatcc accatctcgc gcaaatcacg cagcaaccat gctcgaactg gagcactgaa
      301 cccggctttc tttcgataca gaatctgttt cgggagcaaa tcttcgaggc agcgcttgag
      361 gataaacttt cgtgttgtac cgtggagctt gtactccgcc ggcacacggg cgctgagttc
      421 gacgagcata tgatccagaa acggcacgcg gacttcgacg gatgctgcca tactggtttt
      481 gtcagaatag gtcaggttga gacagggaag aaatgtcttt aaatccaggt aaatcatctg
      541 gttgaccggg tgctgatctt tgacccgttc caggtacgtg cggtgcatcc gataggcatc
      601 aaatgtgctc gccgtggccc gcaattccgg cgaatagagc gcctgtttct cagcatcggt
      661 gaaataggtg ccgtacccga gatagcgttc ctggaagggc aacgcggcag atttggcgag
      721 ttttttgaca ttgcgcgaga gcttgctgag cgtgccggcg cccgcgccag gcaacattgc
      781 caccagcggc tggctgatcg cttgcgggac gaggttatac attttggcca gtcggactgc
      841 ggccaggtgg cgcgggtagc cggcaaaaat ttcatcggcg cccattcctg acagcaacac
      901 agtgagtgtt tcgcgtgctg cccggcaaat cagatagctg gtcaatatcg ctggatccgc
      961 caccggttca tccatatgcc agaccatttt cggcagcaac tcaattacat ccgggcggat
     1021 gatgctttcg tgatagtcgg tctcaaagag ctttccaacc tgacgggcat aggccgtatc
     1081 gtcttcattc acatcatagg cgagatcttc gggcgtgctg ccgacggtgt aggtctgtac
     1141 ttttcgccgt ggggtttgcg tctcaaacat ccgggtcatc agccccacga tgcttgatga
     1201 atccacgccg ccgctcagaa acgtgcctaa cgggacatcg ctcaccaggt tgcgctcaac
     1261 cgagagcgtc aattcttcac ggacggtcgc ttcccacgat ttcaggctta gcgaatcgtc
     1321 ttcggcaaac gtgacgtccc agtaaggctg aaccgtcagt tttccgcgtt gccacatcag
     1381 aaaatgccct ggcggaagtt tgaagatgtt gcggaacgtg gtttttgggt ccgggaccca
     1441 gaggaaggtc aaataatcat tgaaggcttc gaggtcaagt tcggctttga cgcctggaac
     1501 ttgcagcagt gacttgattt ctgagccgaa ggcaaagcct ccgtggtgcg tcgtatagta
     1561 aaacggcttg attcccagcc ggtcccgagc ggcaaacagg gtgtcggttg tttcgtccca
     1621 aatcacatag gcaaacatgc cgttaagtcg ctgcaggtgg tcaacgcccc actcatcata
     1681 agccgccaga atgacttcgg tgtcgctttc agacttgaag cgatggccga gtgcttcgag
     1741 gtcacgccgg atttcacgaa agttgtagac ttcgccatta taaatcatcc accggcggcg
     1801 agtggcattt ggcatggggt tatgcccggc gggcgacgtg tcgagaatcg ccagtcgctg
     1861 gtgacccaga gccactgttt cgccagttcc ggtcggtttg acgtacgttc cacggtcatc
     1921 cggtccacga tgctggatag catccagcat ccggatcaaa tctgcttcag ttacttttcc
     1981 aatggcgccg gtaattccac acataagatc ttttagggtt cagggttcag ggttcagggg
     2041 gttaaaagac ataaaggaca taaaagacga aaaggacata aaatcgaaaa cccggaaccc
     2101 ggaacccgga acccggaacc cggaacccgg aacccggaac ccggaaccct cagttttcta
     2161 atcaattctg aagatccgat tggcactgcc gttgatctcg actgtgagca aggtctggct
     2221 tccgggaacc agggccaccc gtcccggtgc gccagggagt tgaatctgat tgagtgtttg
     2281 accctggttg atattaaaaa acagaagcga ttgcagccca taggacgcaa tggccccgtt
     2341 ttcattgtcc gcaaaaataa ctggtaaatt gctgacaata aaatagttat tttggggtgg
     2401 cttgatgcgc tggcttttgc gaagcccctg tgtctggtcg cgttcgagaa tcaggatttt
     2461 cccgtcatcc agattggcgg tgataaacgc cggtttgccg tttttctcaa acgggttgag
     2521 gacgacaccc gtgatccctt tgatctgatt gacaatctgt tctttggcca catcaatcac
     2581 cagcaactcg ccggaaaatg aagcccccag atagagttgt ttcccgtcag tactaaaaag
     2641 cggacggtta tcaagcgcca gttggatttc tttgtcaacc cgggtggttg acactggctg
     2701 catcgcgcgc gggtcgttga cgttgagcac cgtgatggtt gagtcctcac caagcgaacc
     2761 agccgtcaca gccagtaact gattgccttc cagagtttga acaaacccaa gttgagccgg
     2821 aaagggacca acttcgagcg aactcaagac cgcaccgcca tcggtttcaa acgcatagac
     2881 tctcgtttca acatcagtga caaacccggt tttgccatca gggctgaaga cgacattgga
     2941 gtaatcaagc aaaaagggat ttgaggtgtt gatgttgacg ctgccggttg tttcccagtc
     3001 accagtctgg ttcaggcgaa ataagttgac gcttgagcct ccgcgagtgg tcaccgccag
     3061 tcggcgctcg gtcgcatttt catagattga cagggtttcc ggattgtcat cagttgtcag
     3121 ccgggcagta acagcattgg tttcaagcga aatttcaaac acttcatccg tcagtggtga
     3181 agcaatatag gctttggttc cgtcagcggt gaccaccggg acctgcttga tggtgaaggc
     3241 cacgccggtt ggcgtctcaa agtttccaac tggttcaaat ccatttggga gagatcgcac
     3301 cagatcaact cgtcgctcgc tggtggtcat aacggcacaa accggatttt ctgaggtggt
     3361 tccaaccgca aaagcaaatg gaatttcttc ggaaaccttt gagctaccaa ggcgttcccc
     3421 ggtttcgatg tcaaaggctt cgacccgctg ttcaaacggg acaagcgcat atccacgggt
     3481 gccgtcaggg ctgaagcgtg gtgggctata caaaagctgc tcagcggtaa actcaacttg
     3541 tgagacctgt ttgggggcca gcggttcagt caggtcaagc accgtgatga gggcttcgga
     3601 atcgtgctga accagcatcc ggttgccgcg tcgttgcaga cgagctggaa aaatcggcaa
     3661 ggtcgtgcgc gataaaattt cagccgtggt ggtgctaaac gcgagcaact gcccacgggc
     3721 aatcgcgatg tagccgaaat cttcgctgtc actgagcagg acggtgttgc tcagcgacag
     3781 ggcgccacga tcaggaaacc ggtacaccgc ctgctgccgc agggcgcgag ggttggtggc
     3841 atccaccaga aacagattgt gtgggccgtc aggtgtgctg gtaatcgcaa gcaggcgttt
     3901 gccgtctttt tcgcggagtg ccagttgcac cgggaaatct cccagggcga gggttccaag
     3961 ttgctcgccg gtctgggtgt caaaggcaaa gagcgtccca cgcccttttt tatcaaccac
     4021 tgtgccaaat accacctgtc cgtcacgact gagcgacaag gtttcctggg taaagtacgc
     4081 agatcgtttt tcaggaaaga ggcaggttga aaccaggtgc ggcacacgcg gattgctcac
     4141 atccacaatc gaaattgtcg gttgcggctg tgaaagaaag gcgttgttaa ataccgccgc
     4201 cagtgttcct tgaacaatga cctgggttgg gccatcgcca acggttactt ccccaagctg
     4261 ttcacctgtg gtcatgtcaa aagtaaacag cgagtcagtt tcatatgatc cgaccagccc
     4321 aaaggtacca tcatcactga cgaccagatt gttgttgaca ccagggaaga acgcccgtgg
     4381 aaacgccagc gtgttgattt gggaggtttg attggaagct gaaaacgccg cgtcggaacc
     4441 accagttgtt tcaggatgag cctgagccgt cataacttcc agttgtggaa ctgcctgatt
     4501 gatcccggtc gcgagactta caatgacggt aaagatgaaa aaaaatcggt aaaacttcat
     4561 aaaaaatcca ggaaaagaat gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnngtaaaga tgaaaaaaaa tcggtaaaac ttcataaaaa atccaggaaa agaatgaaga
     4741 atgaagaatg aagaatgaag aatgaagaat gaagaattgg ttggtttcta cctgtaatag
     4801 caatttactt gtttcctgtg tctcaggtaa tttgttgaaa aataagataa acgctcaaaa
     4861 tagttgaacc accattgctt gtcattccag ttattcatca agtgcccatc agttttttta
     4921 ttctcaattc ttcattctca attcttcatt cttcattctt ttgctcttca agcaccttga
     4981 taatgacaac ggtcaaatcg tcgtcaagga ccgactgctg ggcgtatttg cggacagctt
     5041 cataaatctg gtgaaccatt tcgcgggctg gcaggtggcg caactctttg acaatgttga
     5101 tcaagccctg ggggccgagt tcgacgccat cgcgagccgc ttcggtgaca ccgtcagtat
     5161 agagaatcac gatttctccc ggtttcaacc ggcgattgca ttcacgatag tcaatgtctt
     5221 caaacattcc caaaaccgtg tttccacacg agagttccag gtgggtgttg tctgcccgga
     5281 gcaaaatcat cgggttgtga ccggcgtcag catacgtaaa ccgatgcgaa aacggatcaa
     5341 gcaccccata aatcgctgtg acatattggg ccccggtcac gctttcccag agcagatagt
     5401 tcacctttcg aaagatgctc cgaatggcgt aatcattgcg gacctgggcc cggagcgaag
     5461 cccgaaaact ggccataatc agagccgctg gaatcccttt tcctgaaacg tcggcaatac
     5521 aaatacccag ttgctgattg ggaaatgaga taaagtcaaa tgagtcgcca ctgacttctt
     5581 cagttgggaa attggcgcca gaaatatcga atccgggaat cacaggatcc cgatctggca
     5641 gcaagctcaa ttgaacctgg cgggcaatcc ccagctcact ttcaagtcgt ttcttttcaa
     5701 ggcgttcctg gtgcagaaag gctttttcaa ttgaaatggc agccgttccg gcaaaaaaca
     5761 tcagcatatc caggtcgcgt tcggtgtaag tggttttttc gtggctttcc aggttaaaag
     5821 caccgataac tttgccgttt gaaataatcg gggccacgat ttcagatttt gtttccgggt
     5881 cggcttcgat atagcggctg tcgtttcgaa catccgggat gatcacacct ttgctgtttt
     5941 tgatgaccca gccgaccagc ccttcgccaa atttcagccg cagcatgttc gttcgcgaga
     6001 cgtcataggc ccgatgaacc agcgcctgca ttcgcttttc attggatttg ctttcgacaa
     6061 tatagattgc cgccgcgtca tagcggacaa acgagcgcac ggcatcaatg atcaggttca
     6121 gaatttcatc aatatcgagc gatgtgctga ttttgtgagt gatttcctga ataagctgga
     6181 atttgatctc cgtcgaaagt gtgtcaacgg caggaaaatc aataaatgca ggtgatggat
     6241 cggcagagta catagaagat gataaggggc acggtgattg atgaaattac ctgggctaag
     6301 ggcttggggc tgagggttaa gaatttgaga caccagaaag aataaaggat agcaaataaa
     6361 atcaactcga catttttccc aatttcttca gcccttagcc ctcagcccca agccctacat
     6421 tcccatccgc aggttgcgga gctggttacc ataggtcagt tcaaacacaa agatgcgacc
     6481 ccgatggtcg caaatcgtca gtgtatcatc atttgtcaac cggagcgaaa tctctctcgt
     6541 ggcattcaat agaagctcaa gccgcgtctg ggtgtgggcc cagtcgagca catagacacg
     6601 aacttcgttt tcggtgcgta cgggaataac catccagttc gtggtcagaa ctggctgatt
     6661 tggcgagagt gcggtggcag tggtcagttc ctgtttgctt aagaatgatt tttgccagca
     6721 acgggttacc tggacaagat gtgtgtcggt ttggagagcg gcattcaggg aaaatccacc
     6781 tggattcaac gccacagtgc gctcaagcac cgggtccgga gtgaacgtga tggcttcgcg
     6841 actttgcagg cgaaactggg gcagcgcata ggtccagatt tcgacctgtt tatgctggcc
     6901 attgacaaca aataacgaac actggctggt cgaacgagag atcgtcagcg ttcggccaac
     6961 ttcgggaatg cgccacaggg cctcaaagcg tttcccgagt gtgtcaatca ccagaatgtc
     7021 atcccgtgaa ttgactaccc aggtctgccc atcaaaatcg cgggcaaaat cagagagttc
     7081 agcttcacac cagtccgagg cgctgcgagt ggtcagatca agtttggtca gtttccagac
     7141 ttcgccacgt cggatcaaac caatcgcccg gttgccctgg tccgagagca ccagtgctgt
     7201 ggctggtttg tcaaagtggg tcagtgtttt cccttgattt gagagcaacc gaatccctga
     7261 ttccccaatc gcgaccaggc actttccatc cggcaggtag gcggcatcat acactggcat
     7321 caggccagtg tccgtggcgt ccgccgtaaa aatccgaggt ttggggctgg tttgcaattt
     7381 tggcagatgc gggctggtta cggttggaac atccgtgcga agtgccgcat cgctggcaaa
     7441 agtcagcaga ttggtgagtt cattggtggt ggtttgaatg tgcccctgac tcatatcccg
     7501 aatcgttgcc cgaactgcgg ctctggcaag catttgtggc accgggcctt tcggggcatc
     7561 ggccaccagt gttcgccgaa atgccgcccg aagtggggct ttttcagcat cttcctcagc
     7621 cagcaggtcg aggatcggcc cgctcatcgc ttcatggtcg gttggaaaga gtgtcagttt
     7681 atacgccagc atttgacctg ccgccacgcc acctccggca atggcacatt cgatccaatc
     7741 cgccgcaatg tggcgagctt cggaaactgg ccagattgtg ttgaccgctg ccggatattc
     7801 acctgcctca gcctgagtcg tggcccagag cagtcggagc gtgtttgcct gttgcggatc
     7861 ggttttttca agtcgaacaa tggcatcggc aaaggcaccg gttcgccggg caagtgtgac
     7921 tgctcgccgc cgatttccag ccagaaacca ctggcgcacc acgagtccgg cagcgagttg
     7981 ccgggcttcg gccatttccg ctgccaatct gagcctgccg tggcgttcca gaaaggcaac
     8041 ggcttcttcg ttttcctgca ggagttcggc caggtgcggg ccgtcaagga gacgccgggg
     8101 ctcggggtga agatcccctt catccagaac gtcgtcttct acgacagccg catccagggc
     8161 atcgacacgc cctcgctcga ggtgacgccg ctggacgacc gccggctgat cgtcgacgcc
     8221 ttc
//
LOCUS       DAOQDO010000018         7868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5146_length_7868_cov_5.178677, whole genome shotgun sequence.
ACCESSION   DAOQDO010000018 DAOQDO010000000
VERSION     DAOQDO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5146_length_7868_cov_5.178677"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4883
                     /locus_tag="PKZ53_00680"
     CDS             <1..4883
                     /locus_tag="PKZ53_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNJ38976.1"
                     /translation="QTGVIEVTSAGAMGRSRDMFQVDGFGYRLVGVPNTAFVLDGSET
                     AYVIHVNAGGELFQSLVELKVKEAPQGVTATFDRKWITAGATATLKVAVAKGKVLPGS
                     YKLMVEGEAAVGGGMVRREVELTLQVKPLLRTALTGRVLSTEMEPIPGATVSLDGRSA
                     TTDGAGMFVLEGIEAGQNRPVMVDGRTATAPNRTYPVIVEPATVIFNRANLVPYTFFL
                     PAIDTQYEVPVAPGQETIVTTPRVPGLKLTIPAEANLRNRDGSPVTRVSLTPVAIDRT
                     PAPLPAGINLTMVFTAQPGGADSDVAMPASFPNLTGELPGTSFSLWNFNHDSAQWEVY
                     GVGQVSPDGRTIEPDINPSTGKPFGLPYFSWFGPGRNLGDSANGPGPGGPYPGGGGDP
                     ANCPSCSSDNPVNFATGFKFDIETDLGWGGARGGLSLTRTHLNSTRIGRFGMGWTDNW
                     TLSVSGSLDGFSASMTVSGQNRPWLMSLVRTEELEGAVEPVKVYESTVQAGMLGDELK
                     YYPERSRNGINEPAFWEYRSKGGQRMKFNGAGRWESMSDRNGNTTVLSYDDRGRLVKV
                     TDPVGRWIEFTYRAASRTPGTENQVVAVTDVMGRRWEYKYGFEFLDETPAVTVFRLEA
                     VTDPLGHRMQYVYFLGSPILRQVIDKRGNVAKTLGYESTGRITGQRFADGGIEAYSYT
                     VSGGQVTGVTITDPMGRVTSRRFNTAGYVIETTDELGQVRRVEREIGTNLPMATIGAD
                     GKIESQEKYDSRGNAVEMTDEFGHTRRMDYESVFNNLTKMVDKIGHETRMEYDEHGND
                     TVVINALGEITRFEYDQFGQLNSLTDGLGHTTRLSYDQYGMQISQTDPLGNQMTYEYN
                     VFGWPLTVTDGEGRHIVATYDKLGRVVQVKDASGAVRQIEYDENGNLITQIDPFGRVW
                     RNEFDVLNRLTRSIDPLGREEQVQYNFNDEVLLTISPSGRIVRYDYDSRGLVKSKIDP
                     ESNQVNFSYDSEGNLISIADKRGYTHSFQYDELGRLIEAQDPSGKSRRRTYDVEGNLK
                     ILIDRLGRQMEIEYDALNRPFIAHYPDAEVTWEYDAAGRVIRLTDSQGGEILYTFDDA
                     DRFVSEQTSTGVIHYQYNQANQRTQLRIPGQSPVLYRYDEAGRIQSITQENNIFLYGY
                     DLASRLTSIQRPNGIITTYSYDDVHRLKTLTHKNQSNQIVDDFHFSYTSDDEISAHFS
                     SFTGINLPEDQNMGMADPMNRINQLDTTIYSFDAIGQPMTKTDERGITRFEWDTRGRL
                     TRVTTPQNQEIKYTYDAVGRRTQRQVGNMTTSFLYDGSEVVADLKNDGKRVDYLNQLG
                     TDQKLVQRENNQPPLFFLPNHQQSTIALTDSKGDITQRIRYEAFGKTQGSALTRYTFT
                     GREFEESTGLIYYRARWYDPQHGRFLTEDPLRFQAGINFYTYTSNNPILLNDPFGLQS
                     QMPSGVDQMMNAGCEESADEAARKAFIEIFGTIYFNNGSLESEEFGGFICCLERKSEK
                     TPCYIFTPPQKLGSGHVGNSIGLTGLGGSGTLRPNGEKACRKEKARQSKAGEIKVVAW
                     YHTHGYNSPKNNKDGTPNNSNEFFSSKEDNGDIALIDSTGLTGYLATPRGKLLVRYPH
                     KRTRTICTGCFPHKKE"
     gene            5010..7013
                     /locus_tag="PKZ53_00685"
     CDS             5010..7013
                     /locus_tag="PKZ53_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ38977.1"
                     /translation="MPLPHFSTQKTPARNHKLAQIGNELTKAVSDRDFEKIKHLASQG
                     IDLMTPDDQNQTAFEQALDKNDAEMVDFLLNQGIDVHRSYRIHPISSDGNGNRVQEET
                     LLIKAVERGNPIIVQRLIEAGSDVNKPNSHGETALSMAVNSLCNKQLVELLLKAGANV
                     NSADKEGYTPLYDAAYSNCDQITRILLQAGAHPNSKTNYGMTPLLMALKNSNEVITRQ
                     LLKAGADVNLAGDGRFTPLMMACFRGDPDIIQLLIEAGVRINAQDEQRRTALYYAVGN
                     IRIQATELLLKAGIDQLIQDKDGKTPFIYILEYRAKGSDHGRLIQLLLDHSEARTRGL
                     GIFCAPPKLLTTNRLPRSPVGKLNWAILNRDVPQALKLIKDGAFRVPKSKRNQEYPPL
                     AYAIRENLPELVAVLIEAGADVNWKHPGWSKRKKIDLLLDSFRKETSYLIMALLFDQL
                     EISRHLIKAGIDLEDEDGIGNTALSTAAEKGYIEIIEALITRGVKVDGPRKFPSPTPL
                     QIAVDFRQEQSVVALLKAGANPNAVDHNGDTPLIRAISARLPNYVKLLLEAGGNPNLP
                     NEGESTPLKFASLLGNEEIVTLLLKAGGNPNYQNKQGQTPLYEASVRKFEAGVKLLLE
                     AGGDPLIKTNNGKTILETTQELQAKNGGYEKILQLLLSRTDSE"
     gene            7542..>7868
                     /locus_tag="PKZ53_00690"
     CDS             7542..>7868
                     /locus_tag="PKZ53_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ38978.1"
                     /translation="MSLKVNWEQYMKKAALWLRYSICSFLLLLLNVSPTDGWNHSQGK
                     DKLKTAADMLTEAVNKGDMAAVKKVLQSGSVNARNSHGETALIAATQKNDFEMVKYLL
                     SIGAKVN"
BASE COUNT         2278 a         1689 c         2068 g         1833 t
ORIGIN      
        1 cgcaaaccgg agtgatcgaa gtcaccagtg cgggcgccat gggacgttcg cgggatatgt
       61 tccaggtgga tgggtttggg tatcggctgg tgggtgttcc gaacacggcg tttgtactgg
      121 acggaagcga aacggcctat gtgattcacg tcaacgcggg tggtgagttg ttccaatcgc
      181 tggtggaact caaagtgaag gaagcacccc agggcgtgac cgcgaccttt gaccggaaat
      241 ggattaccgc cggagcgaca gcaaccctga aagtcgccgt ggccaaaggg aaagtgttgc
      301 caggaagcta caaactgatg gtcgagggcg aagccgccgt tggcggaggg atggtccgcc
      361 gggaagtgga actgacgtta caggtcaagc cgctgctccg aactgcgttg accgggcgtg
      421 tgctgtcaac cgaaatggaa ccgattccag gggcgacggt ctcgctcgat ggtcggagcg
      481 ccaccacgga cggcgccggg atgtttgtgt tggaaggaat tgaagccggt cagaatcgtc
      541 cggtgatggt ggatggccgg accgcgacgg caccgaaccg gacctacccg gtgattgtcg
      601 aaccggcgac ggtgattttc aatcgggcga acctggtgcc gtacacgttt ttcctgcccg
      661 cgattgacac ccaatatgaa gtgccggtcg ctccgggaca ggaaaccatc gtgacgacac
      721 cgcgagtgcc agggttgaag ttgacgattc cagccgaagc gaatttaaga aaccgggatg
      781 gttcgccggt caccagggtg tcgttgacac cagtggcgat tgaccggact ccggcgccgc
      841 tgcccgctgg aatcaacctg acgatggtct tcaccgccca gccgggaggt gctgattcgg
      901 acgtagccat gccggcgagt ttccctaatc tgaccggaga actgccggga acgtccttta
      961 gtttatggaa tttcaaccat gactccgcac agtgggaagt gtatggggtg ggtcaggtga
     1021 gtccggatgg tcgcacgata gaaccagaca tcaatccgag cacggggaaa ccatttggac
     1081 tgccttattt ttcgtggttt ggtccaggcc gcaacctggg tgacagcgcc aacggtccgg
     1141 gtcccggagg tccctatccc ggaggcggcg gagacccggc gaactgtccc tcgtgcagca
     1201 gtgacaaccc ggtgaatttc gcgaccgggt tcaaatttga catcgaaacc gatttgggat
     1261 ggggcggggc gcgtggtgga ctgagcctga cccggacgca cctcaattcg acccggattg
     1321 gacgctttgg gatggggtgg accgacaact ggacgctgag tgtgagcggg tcgctggatg
     1381 gattttcagc ttcaatgaca gtgtcaggac aaaaccggcc ctggttaatg agcctggtgc
     1441 gaaccgagga actggaggga gcggttgagc cggtcaaagt ctacgaaagc acagtccagg
     1501 ccgggatgct gggggatgaa ttgaagtatt atccggaacg aagccggaat ggaataaacg
     1561 agccggcctt ttgggaatac cggagcaaag gcggacagcg aatgaaattc aacggcgcgg
     1621 ggagatggga gtcaatgagc gaccggaatg ggaacacgac ggtgttgagc tacgacgacc
     1681 gggggaggct ggtgaaagtg acggacccgg tgggaaggtg gatcgagttt acctatcgtg
     1741 cagcgtcgag aacccctgga acggaaaacc aggtagtggc ggtgacggac gtgatgggac
     1801 gccggtggga gtacaaatac gggtttgaat tcctggacga gacccctgcc gtcacggtct
     1861 ttcggttgga ggcggtgacg gatccactgg gccatcggat gcaatatgta tatttcctgg
     1921 gttcgccgat tttgagacag gtgattgaca aacgcgggaa tgttgccaag acgctgggct
     1981 atgagagtac gggccggatc acaggccagc gattcgcgga tggagggata gaagcctaca
     2041 gctacacggt gtcaggcggg caggtgacgg gagtaacgat taccgacccg atgggccggg
     2101 tgacgagccg gagattcaac accgccggct atgtgattga aaccacggat gagctgggcc
     2161 aggtccgaag agtagaacgg gaaattggga cgaatttacc aatggccaca attggtgccg
     2221 atgggaagat tgaaagtcag gaaaaatatg attccagagg aaatgcggtt gaaatgaccg
     2281 atgaatttgg ccatacaaga cggatggact acgaatcagt gttcaacaat ctaaccaaga
     2341 tggtagataa aattggccac gaaacccgga tggaatatga cgagcatgga aatgatacag
     2401 tcgttattaa tgctttggga gaaatcactc gatttgaata tgatcagttt ggacaattga
     2461 atagcctgac tgatggcctt gggcatacta ctcgcctatc ctatgaccag tatggaatgc
     2521 agatttctca aaccgatcct ttaggaaacc aaatgaccta tgaatataat gtttttggat
     2581 ggccactgac agttacagat ggggaaggac ggcacatagt agcaacttat gacaaactgg
     2641 gacgggttgt tcaagtcaaa gatgcttctg gtgcagtcag gcagattgaa tacgatgaaa
     2701 atgggaacct gataacccag attgatccct ttggccgagt ctggagaaat gaattcgatg
     2761 tgttaaatcg cttaacccgc agtattgacc cgttagggcg tgaagaacag gtgcaataca
     2821 attttaatga tgaagtactc ttaacgattt caccatcagg aagaatagtt agatatgatt
     2881 atgattcacg aggactggtc aaatcgaaaa ttgacccgga aagcaaccaa gttaattttt
     2941 cctatgatag tgaagggaat ttaatttcta ttgcagataa aagaggatat acacactctt
     3001 ttcaatacga tgagcttggg cggttaattg aagcgcagga tccttcaggg aaatcaaggc
     3061 gcagaacata tgatgttgaa gggaatctga aaattctcat tgatcgcctt ggaagacaaa
     3121 tggaaattga gtatgacgca ttgaaccgtc cttttattgc tcattaccca gatgctgagg
     3181 ttacctggga gtatgatgcg gctggtagag tcatccggtt gactgattct caaggaggtg
     3241 aaattcttta cacttttgat gatgctgacc gatttgtctc cgagcaaact tcaactggag
     3301 tcattcatta tcaatataat caggccaatc agcgaaccca actcagaatt ccaggccaat
     3361 ctcccgtcct ttataggtat gatgaagctg gaagaattca atcaatcacc caggaaaaca
     3421 atattttttt atatggctat gatttggctt ccagattgac atcaatccaa cgcccaaatg
     3481 gaattataac gacctatagc tacgatgacg ttcatcgctt gaaaaccctg actcataaaa
     3541 accaatcgaa ccagattgtt gatgattttc atttctcata tacatcagat gatgagataa
     3601 gtgctcactt ctcatcattt actggtatta acctgcctga agatcaaaat atgggcatgg
     3661 ctgacccaat gaatcgaatc aatcaattag acacaactat atattccttt gatgcgattg
     3721 gacagcctat gaccaaaact gatgaacggg gaattacccg ctttgaatgg gataccaggg
     3781 ggagattgac ccgagtcacg actccacaga atcaagaaat caaatacacc tatgatgcag
     3841 ttggaagaag aactcagcgg caggttggaa atatgacaac atcattcctc tatgatgggt
     3901 cagaggttgt cgctgacctg aaaaatgacg ggaagcgggt tgactacctg aatcaattag
     3961 gaacggacca aaagctcgtt caacgggaaa ataatcagcc gccccttttc tttctgccca
     4021 accatcaaca gagtacaatt gccttgacgg attcaaaggg cgatatcact caaagaatca
     4081 ggtatgaagc ctttggaaaa actcaaggaa gcgcattgac ccgatataca ttcactgggc
     4141 gggagtttga ggaatcaaca ggactgatct attacagagc acgttggtat gacccacaac
     4201 acggcagatt cctaaccgaa gatccactac gattccaggc aggaattaat ttttacacat
     4261 acacgtcaaa taacccaatt ttattgaacg atccctttgg acttcagagc caaatgccaa
     4321 gtggcgtgga ccaaatgatg aatgctggtt gtgaggaaag tgctgacgaa gcagcaagaa
     4381 aagcgtttat tgagatcttt ggaacaattt acttcaataa tggcagtctg gaaagtgaag
     4441 aatttggtgg atttatttgc tgtttagaaa gaaaaagcga aaaaactcca tgttatattt
     4501 ttactccacc tcaaaaactt ggaagtggac atgtagggaa cagcattggg ttaacagggt
     4561 taggaggtag tggtacatta agaccaaacg gggaaaaagc ttgtagaaag gaaaaagcaa
     4621 ggcaatctaa agctggggaa attaaagtgg tagcttggta tcacacccat ggctataact
     4681 cacctaaaaa caataaggat ggcacaccaa acaattcgaa tgagtttttt tcatctaaag
     4741 aagataacgg tgacatcgct ttgattgact caacgggtct tacaggatat ctagccactc
     4801 ccagagggaa gctcttagta cgttaccctc ataaaaggac aaggacgatt tgcaccggtt
     4861 gtttcccgca taaaaaggaa taaaaagaaa tgtcatgaac acttatttat gtttgttgcc
     4921 tgtgaccagt gtcggataat tgatgaggtt caaccatgaa gcaaatatta tttcacaagg
     4981 tgctttgtat atttattgtt gttttttatt tgccccttcc ccattttagc acccagaaaa
     5041 cgcccgcccg caaccataaa ttagctcaaa ttggaaatga gttaacaaag gcggtttctg
     5101 atcgagattt tgagaaaatc aaacaccttg ccagccaggg aattgatttg atgacgcccg
     5161 atgatcaaaa ccaaactgct tttgagcaag ctcttgacaa gaatgatgct gaaatggtgg
     5221 attttctgct caatcaaggg atagatgttc atcgaagtta taggattcat ccgattagct
     5281 ccgatggaaa tgggaaccgg gtccaagaag aaacgctcct gataaaggct gtggagcgag
     5341 gcaacccaat cattgttcag cggctgatcg aggcaggaag tgatgtcaat aaaccaaact
     5401 cacatggtga aacagcgcta tccatggctg tgaactcttt atgcaacaaa caactggtgg
     5461 aattattgct caaagctggt gcaaacgtaa attcggctga caaagagggg tacacacccc
     5521 tttatgatgc ggcttatagt aattgcgacc aaatcacaag aatattgctc caggctggag
     5581 cccaccccaa tagtaaaaca aactatggga tgactccgct tttaatggcc ttgaagaatt
     5641 caaatgaagt gatcaccaga caattactaa aagctggagc agatgtgaat ctagcgggcg
     5701 atgggcggtt tactccctta atgatggctt gttttcgcgg ggatccagac atcattcagt
     5761 tgctcatcga agctggtgtc cggatcaatg cccaagatga acaaaggcgg acggctctct
     5821 attatgccgt tggaaatatt cggatccagg ctactgaact cttattgaaa gctgggattg
     5881 accaactgat acaggataaa gatgggaaaa ctccctttat ctatattctt gagtacaggg
     5941 cgaaagggag tgatcatgga cggttgattc aactcttact tgatcactct gaagccagga
     6001 ccagagggct aggaattttt tgtgctccgc caaaactcct cactactaac cgtctgcccc
     6061 gatctccagt cgggaaactc aattgggcca tcttaaaccg ggatgtgccg caagccttga
     6121 aactgatcaa agatggggca tttcgggtcc caaagagcaa aagaaaccag gagtatccac
     6181 ctctggcata tgccattcgc gagaatttgc cggaactggt tgcggtgctg attgaagctg
     6241 gagctgatgt caactggaaa caccctggct ggtccaaaag aaagaagatt gatcttcttt
     6301 tggattcgtt tcgaaaagaa acctcctatt taatcatggc cttgctcttt gatcagttgg
     6361 aaatcagccg ccatcttatc aaggctggaa ttgaccttga agatgaagat ggaattggga
     6421 ataccgcgtt atcaactgca gccgaaaaag ggtatatcga gatcatcgag gcgttgatca
     6481 cgagaggtgt gaaggtggat ggaccacgga aatttccttc tccaacccct ctccaaatag
     6541 cggtcgactt tcgacaggag caatctgtcg tcgcattact caaagcaggt gccaatccaa
     6601 atgcagttga ccataatggc gatacgccct tgatccgtgc catcagtgct cgattaccca
     6661 actatgtcaa actgctactg gaggcaggcg gaaatccaaa tcttccgaat gaaggagagt
     6721 ccactcctct aaagttcgcc agtttattgg gaaatgaaga aattgtcaca ttgctgctca
     6781 aggcgggagg taatccaaat taccaaaaca agcaaggcca aaccccgctc tatgaagcgt
     6841 cagtcagaaa atttgaagcc ggtgtcaaac tcttgctgga agctgggggt gacccactga
     6901 taaaaaccaa taacgggaaa acgattctgg aaacaactca ggaacttcaa gccaaaaatg
     6961 gggggtatga aaaaattctc caattgttac tgagccggac cgattcagaa tgaatgccgg
     7021 gtgctttttg aggtgggatg gtaacctcaa tcttttttcc cttgatgact tgaatattac
     7081 tggcaaaagc atctcccacg accataacca cttccggggg agtttccccc tggtcacgac
     7141 ctggccacta ccttcaagcc gatcagatgt aactgagatg ttgtggatga tggatgtctt
     7201 ctcagctatg gcttaaggat tgtgaaaaaa atcacgtaaa atttgctctc ccacaatttc
     7261 gctgatggcc agccagctac cgggtaaaat actcctcagg tagatcggag tggaaacagg
     7321 gcagaactga ggagtgaatc tggaatcaat gattttgatg gtaccagcgg aagcccaact
     7381 tgagatggaa tctgtcaaaa gggggtagaa agatgccact ttacgcaaag aatgcgcaaa
     7441 agacccgcca aaaccagtca accgtgactg aactgttgcg gtgttaaaga aaattcgaac
     7501 tcaaagcctg aaatccatca atcggtgttg ataaggatac gatgagttta aaagttaatt
     7561 gggaacagta tatgaaaaaa gctgctcttt ggttgcgtta cagtatctgc tcatttcttt
     7621 tgttactgct gaatgtttcg ccaactgacg gttggaatca ttcacaaggc aaagacaaat
     7681 tgaaaacagc agcagatatg ctgacagagg ctgtcaacaa aggtgacatg gctgcagtta
     7741 agaaagtgct tcaatccgga agtgtcaatg ccagaaatag ccatggagaa accgctctca
     7801 ttgctgccac ccaaaaaaat gactttgaga tggtaaaata tttgctttct atcggagcaa
     7861 aggtgaac
//
LOCUS       DAOQDO010000019         7834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5188_length_7834_cov_3.138193, whole genome shotgun sequence.
ACCESSION   DAOQDO010000019 DAOQDO010000000
VERSION     DAOQDO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5188_length_7834_cov_3.138193"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="PKZ53_00695"
     CDS             complement(<1..2016)
                     /locus_tag="PKZ53_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ38979.1"
                     /translation="MSLFGTSYSKEKVLKSAEKYIQQGKLDAAIKEYKRILEVEPQDL
                     KVVSSLAELYSRSNQTREAVTLFLRVADHYERTRQVPQAIAMYRRIFKMDINNYPAAV
                     TLAELYRQQGNIPEAVQFYLGAASACRRTNQIPTAIKMAREAVSLDPESIRARVELAG
                     VLHLSEEYDEAHDLYVQAGREFLRKGQNNEGLECFRKAMLVKPGSKPALKALTDISLQ
                     MGDAKGAVRMVKQLLVSNPQDIDLLILLGRTCLGGNLLDDAEEAFTSLVQLDPQRYEY
                     LLEVGRQFLKIGMFDRAIHTVQKCAEHVVARGQKKKATAILKHIIQMDPNHLTAMEVL
                     ADVYEKAREKRNLSTTLNLIVEVAQRQGKNEEALTALKRLIAIAPKKKNYREQLAQLG
                     QQATMVMQAIKPDEDFEDDLDQYNYSTELLESMVQQNPAFAQARIKMLEDMAAQQPDY
                     LEVWQQLRQNYLDSQLKEKAAKACLELARIHLARGNQDAAETAKAEAYDLDPGLKPVQ
                     PMIQVGEGESVPLTDIMPDVGIVANVVSSSSATTVSEDDQTVALRALPGTFNRYLTVD
                     KFQKRYEGLWTDPIYSTEPLSLVKIQVDNFVGVVEENEEIRESYLQPIAETLHDEVKT
                     PNAYIAYCGNATFFAFFPKVNPDTIEHLAESMRNRVESLWLPHPSSDD"
     gene            2250..2660
                     /locus_tag="PKZ53_00700"
     CDS             2250..2660
                     /locus_tag="PKZ53_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38980.1"
                     /translation="MQIEYLVLGVVAIATLSGWAYTFRLWRRMHLTRQSWETSHKRTS
                     QQISRLETQLARVSTELKALKKEETRLNIQVRELTNWLAINTCNCGCQHESNPEKHHR
                     LCEFRLALLFVIATVTEHTSPPPKIKSALFARGK"
     gene            complement(2677..3747)
                     /locus_tag="PKZ53_00705"
     CDS             complement(2677..3747)
                     /locus_tag="PKZ53_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNJ38981.1"
                     /translation="MNQPFLDQPRKIRQGEELEVENLQEFLKKHIPDLAEPLEIEQFP
                     GGYSNLTYFLKAGSVEMVLRRPPFGAAIKSAHDMGREFRILSSLATVYGKAPHPVAYC
                     EDTAVIGAPFYVMERVKGIILRSRKPKGLDLTPEIMHDLSTACIDNLAEIHGIEYCAA
                     GLTDLGKPEGYVARQVRGWTERYHNARTDDVPAIDRAIEWLATHQPEHSGAALIHNDY
                     KYDNLVLDPDQLTRIIAVLDWEMATIGDPLMDLGTTLGYWVDPDDPDELQQAQFGLTT
                     LPGNLCRRELVDRYAITSGRDVRQVVFYYVYALFKIAVIVQQIYKRFRLGHTTDERFA
                     HLDVIVRVLGASAVRAIQRNQI"
     gene            4242..5138
                     /locus_tag="PKZ53_00710"
     CDS             4242..5138
                     /locus_tag="PKZ53_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ38982.1"
                     /translation="MKLLLALDGSPNSQFALQQACARPWPIDTVIGVLSVVDPKSFEV
                     SPAELPARVKAETEIAHQLVLEAAARLNRNQVETTTLVAEGDPRSVIIKYARLWSVDT
                     ILMGALGKSQIPKFFMGSVAKAVLRESPCSIWIARPRAEAPLPSPSVGMRVMIATDGT
                     AEAQAAIDSVANYPWPAGSLFYVVTVAEPARSLAAHWAEPSELSSKIAPEVLERAQVA
                     IRAAFKSLEGNEYGLSSSVLVGYAKEAILEEAANWNADIVVVGTRGWSGIDRIRLGSV
                     SDAIAIHAPCSVEIVRHCTLSE"
     gene            5271..5873
                     /locus_tag="PKZ53_00715"
     CDS             5271..5873
                     /locus_tag="PKZ53_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNJ38983.1"
                     /translation="MTVPLQITFRNMESSEAVETRIREEVEKLQTYFDQIISCRVVVE
                     IPHRHHQQGNTCLVTVDLHVPGSEIVVKQEPTLYNSLQHTGAPKTSKQVEISGPHKDM
                     YTTIRDAFKGVRRKLQDFVHRQRHQVKQHEETPLATVSQLFVNEGYGFIETIEGELVY
                     FHRNSLNNEDFEALAVGTPVSFIEEVGEKGPQASLVKVIA"
     gene            6233..6607
                     /locus_tag="PKZ53_00720"
     CDS             6233..6607
                     /locus_tag="PKZ53_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38984.1"
                     /translation="MKNFTKHAFSILALSAVLTLPVLAGEKKESITFSRNIVVNGTTI
                     QAGTYDVKFNDQTGEFIILKRGKELAKTTGSLKDRGRKVTSTEVFFSKQGDNAVLSGV
                     TFAGANQTVVVNDGAQAASPSQ"
     gene            complement(6757..>7834)
                     /locus_tag="PKZ53_00725"
     CDS             complement(6757..>7834)
                     /locus_tag="PKZ53_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ38985.1"
                     /translation="TAIIGNVELVRTYVTNPQKILTRLDAVQRASDRAKGLARQLLTF
                     AKGGVPVREIANLEYFIRDTIVFSLHGSNIRAVFQFDPHLWLTEIDPGQMGQVFQNLA
                     LNARDAMPLGGELLVCGKNITLAETGQYPLTPGDYIKISVIDQGIGVNPEHLTKIFDP
                     YFSTKETGHGLGLAIAYFVVKKHNGYITVTSEAGIGTQFDIFLPASQVAQPSLRLATG
                     EIASSESSRPVRILVMDDEEIVRETTREMLEALGYEIEVSKDGIEAVVLYGKAIENHT
                     PFSGVILDLTVPGSMGGQETLEKLRLIDPQVKAIVCSGYSNDPILSDYSQYGFVGKLV
                     KPFSLASLEDACHLFASNDSRSSQ"
BASE COUNT         1938 a         1994 c         1910 g         1992 t
ORIGIN      
        1 atcatctgaa gatgggtgcg gtaaccagag tgattcaact cgattccgca tggattcggc
       61 cagatgttcg atggtgtcag gattcacttt cgggaaaaaa gcaaaaaatg tggcatttcc
      121 acaataggca atataggcat tgggagtttt gacttcgtcg tgcagtgttt cagcaattgg
      181 ttgcaggtag gattctcgga tttcctcatt ttcttcaacc accccgacaa agttatcaac
      241 ttgaattttc accagtgaaa gtggctcggt tgaataaatt gggtcggtcc agagtccttc
      301 ataccgtttt tggaatttat ccacagtcag gtagcggtta aatgtacccg gaagtgccct
      361 gagcgcaacg gtttgatcat cttctgaaac agttgtggct gacgacgatg agaccacatt
      421 cgccacaatt ccgacatccg gcatgatatc ggtcagcggc acgctttcac cttccccaac
      481 ctgaatcatg ggctggacgg gtttcagacc tggatccaag tcataggctt cggctttggc
      541 cgtttcagcg gcgtcctgat ttccacgggc gaggtggatc cgagccagtt ccagacaggc
      601 tttggcggct ttttccttga gctggctatc gaggtagttt tgcctgagtt gctgccagac
      661 ctccagataa tcaggttgct gggcagccat atcctccagc attttgatgc gggcctgagc
      721 aaaagccggg ttttgttgga ccatgctttc gagcaattcc gtgctgtaat tgtattgatc
      781 cagatcatct tcaaaatctt catccggctt gatggcctgc atcaccatgg tggcctgctg
      841 tccaagctga gccagttgct cgcgataatt tttctttttg ggggcaatcg caatcaggcg
      901 cttgagcgcg gtcaaggctt cttcattctt tccctgccgc tgggcaacct cgacgattaa
      961 attgagtgtg gttgaaaggt tgcgcttttc cctggctttt tcatagacat cggccagaac
     1021 ttccatggcc gtgagatgat ttggatccat ctggatgatg tgtttgagga tcgcagtcgc
     1081 ctttttcttt tgcccccggg caacgacgtg ttcggcacat ttttgaaccg tgtgaatggc
     1141 ccgatcaaac attccgattt tcaggaattg ccgtccaact tcaagcaaat attcatagcg
     1201 ttgcgggtca agctgaacca gacttgtaaa ggcttcttcg gcatcgtcga ggaggtttcc
     1261 acccaggcac gtccgcccca ataaaatcag caggtctata tcctgggggt tactcaccag
     1321 taattgcttg accattctga ccgccccttt ggcatccccc atctggagcg agatatcggt
     1381 cagggctttt aaagctggtt tagagccggg tttgaccagc atggctttcc gaaagcattc
     1441 cagcccttca ttgttttggc ctttgcgtaa aaattcgcgg ccagcctgga catagaggtc
     1501 gtgggcttcg tcatattctt ctgaaagatg gagcaccccg gccagttcca ctcgggcacg
     1561 aatagattca ggatcgagcg acacggcttc gcgagccatc ttgatcgcgg tcggaatctg
     1621 attggtccgc cggcaggcac tggctgcccc caggtaaaac tgaactgcct ccgggatgtt
     1681 cccctgctgg cgatagagtt cagccagcgt cacggctgcc gggtaattat tgatgtccat
     1741 cttaaaaatc cgacggtaca tagcaattgc ctgtggaact tgccgggtgc gttcgtaatg
     1801 gtcggcaact cgcaaaaaca gcgtcacagc ttctcgggtc tggttgctgc gcgaatagag
     1861 ttcggccagc gaactcacaa ctttcaaatc ctggggttca acttccaaaa ttcgcttata
     1921 ttccttgatg gcagcatcaa gctttccctg ttggatatat ttttcggctg atttcaagac
     1981 cttttctttg ctataagacg tgccaaacag tgacataagg cgaccttcct ctgaaaacac
     2041 caaagcgagc tgcacgtgcg gcaatgcagt tggttctgat gtgtaatttc aaaactgatt
     2101 gatcggggac gttgagtgag cgaggtaata cacggcaatt cccaatagag tcaaaggctt
     2161 gggatgtctt gtcaagcctt ttttcacggt tctgaatttt cctgaaagga ttggccggga
     2221 caagccacta tgaaaaaagg agaaacaccg tgcagattga atatctggta ctgggggtgg
     2281 tggcaatcgc aacgttgagc gggtgggcgt atacctttcg tctctggcgg cgaatgcacc
     2341 tcacccgcca atcctgggaa acaagtcaca aacgaaccag ccagcagatt tcccgcctgg
     2401 aaacacaact ggccagggtt tcaactgaat tgaaagcact caagaaagaa gaaaccagac
     2461 tcaacatcca ggtgcgagaa ctcaccaact ggctggccat caacacctgc aattgcggct
     2521 gtcagcacga gtccaaccct gagaaacacc accggttatg cgaattccgg ctggcattgc
     2581 tgtttgtgat cgccacggtg actgagcaca ccagcccgcc acccaaaatc aaatccgcat
     2641 tgttcgccag gggaaaataa cccaccgccc ccttgcctaa atctgattcc gctggattgc
     2701 gcgcaccgcc gaagcgccta acacccgaac aatcacatcc aggtgggcaa accgttcatc
     2761 cgtggtgtgg cccaaccgaa agcgtttata aatttgctgg acaatcactg caatcttaaa
     2821 cagcgcatac acataataaa aaacaacctg tcgaacatcg cgcccactgg tgatggcata
     2881 tcggtcaacc agctcgcgcc ggcataagtt gcccggcagg gttgtcaatc caaattgtgc
     2941 ctgttgcaac tcgtctggat catccgggtc aacccaatat ccaagggtcg tcccaagatc
     3001 catcagcggg tctccgatgg tcgccatttc ccaatccaga acggcaatga tccgggtcaa
     3061 ctgatcagga tcgagcacca gattgtcata cttgtagtca ttatggatca acgccgctcc
     3121 cgaatgctca ggttgatgag tggcaagcca ttcaattgcc cggtcaatcg ctggcacatc
     3181 gtcagttctg gcattgtgat accgctcggt ccagccacgt acctgacggg cgacatatcc
     3241 ttcaggtttc ccaagatcag ttaatccggc ggcgcagtac tcaatgccgt ggatttcggc
     3301 gaggttgtca atacaggccg ttgagaggtc gtgcatgatt tctggcgtca gatccagacc
     3361 cttgggcttg cgcgaacgca aaatgatgcc cttgacccgt tccatcacat aaaaaggggc
     3421 cccaatcacg gcagtgtcct cacaataagc gaccgggtgc ggagcttttc catataccgt
     3481 ggccaggctc gacaaaatcc gaaactcgcg ccccatgtcg tgggccgatt tgatggcggc
     3541 cccaaacggt ggccgccgaa gaaccatttc gactgaacca gcttttaaaa aatatgtcag
     3601 gtttgaatag ccacctggaa actgttcaat ttcaagcggt tcggccagat caggaatatg
     3661 tttttttaga aactcctgaa ggttttccac ctcaagttct tcgccctggc ggatttttcg
     3721 gggttgatca agaaaaggct ggttcatcaa agccgtatct cctttggagt gcctggtatt
     3781 tggggctggg ggctggaagc tgaaaccagg ggatgaagga tgagggatga aagaaaaccc
     3841 gtttctcagc ccgacttctt cagcccccag ccctgagccc gatgtcttta accccatctt
     3901 caacattctt gcgggttgat gcaaccgctt caattcaaac cggcggtgaa aattccaatc
     3961 tcgggcaaaa gtcccctggt ttgtggaatt ttacgcccga gggggagaat acccaaatcc
     4021 tgaaaaacta ctaaacactt gattttactc agcttattag aatttgaata ccttccacac
     4081 ccacattgta gaaaaaaaac aggtcatttt gacaatggca taggagttgc tctaagagaa
     4141 gccgagggtt cccaatcagc gtaacaaagg ggcaactttt gtacgttcaa cgctgattga
     4201 gaactctctg aattttgacc gtctccattc tggagtttgc tatgaagttg ctgctcgccc
     4261 ttgatggctc ccccaattct caatttgctc tccagcaagc ctgtgccaga ccctggccga
     4321 ttgataccgt gattggcgtt ctttccgttg ttgatccaaa gtcgtttgag gtgtcaccgg
     4381 ctgaattgcc tgcacgagtc aaagcagaaa ctgagattgc gcatcaactg gtgcttgaag
     4441 ccgccgcccg cctgaatcgg aatcaagttg aaaccaccac gctggtggcc gaaggtgacc
     4501 cccggtcagt catcattaaa tatgcccgat tatggtcggt tgataccatt ctgatgggag
     4561 cattaggcaa aagtcagatc cctaaatttt ttatgggaag tgtggccaaa gcggttttgc
     4621 gcgaatcccc ctgctcaatt tggattgctc gccccagagc tgaagcccct ctcccctccc
     4681 caagcgttgg catgcgggtc atgattgcaa ctgatggaac ggcagaagct caggccgcga
     4741 ttgattcagt ggcgaactac ccgtggccgg ctggcagttt gttttatgtt gtgacggttg
     4801 cagagccagc cagatcactg gcggcgcatt gggctgaacc ctctgaactc tcaagtaaaa
     4861 tcgctccgga ggtactggag cgggctcagg tggccattcg cgctgccttc aaaagtctcg
     4921 aaggcaatga gtatggactc tccagttcgg tgttagttgg ttatgccaaa gaagccatcc
     4981 tcgaagaagc cgccaattgg aatgcggata ttgttgtggt tggaacacgc ggctggagtg
     5041 ggatcgaccg gatccggctc ggcagcgttt ctgatgcgat tgccatccac gccccctgtt
     5101 ccgtcgaaat tgtgcggcat tgtaccctct ctgaataagg aattttctgc aaattgcggg
     5161 tcaaaacccc actggatcta agggaatccc cctgggttga gtgtgttttc cctttggctt
     5221 gcaccttatt caacggcatc aatcccattt tttagaaaga gagacagttt atgacggttc
     5281 cactgcaaat cacttttcgc aatatggagt cctccgaagc cgttgaaacc cggattcggg
     5341 aagaagtcga aaagctacaa acctatttcg accagatcat cagttgtcga gtcgtggtcg
     5401 aaattcccca tcggcatcat caacaaggca acacgtgcct ggttactgtt gatttacatg
     5461 tgccaggaag tgaaattgta gtgaagcagg aaccgaccct gtacaactcg ctccaacaca
     5521 ccggggcccc aaaaacatcg aaacaggttg aaatcagtgg cccacacaaa gatatgtaca
     5581 ccacgattcg ggatgcgttt aaaggcgtgc gccgcaaatt gcaggatttt gtgcaccgtc
     5641 aacgacatca ggtgaaacag cacgaagaaa cgccgctggc aacggtcagc cagttgtttg
     5701 tcaatgaagg ctacgggttt atcgaaacca tcgaaggcga actggtgtat tttcaccgca
     5761 acagcctcaa caacgaagat tttgaagccc tggcagtggg cacaccggtt tcgtttatcg
     5821 aagaagtggg tgaaaaaggt ccgcaggcca gcctggtcaa agttatcgcc tgatttgctt
     5881 tctctcgatt aaagcctctc tccttccaac agcacaccat cggtttcgaa gcgaaataga
     5941 tggtgtgcat ttttttgcgc tctgtaaaac ccttgtaaag cgcctgggca atccgatttt
     6001 catcagccgc tttcggctcg ctccataaca ctgtaatcca acttctcttt tcaaggactt
     6061 aggaaatggt tcaaaatgaa atgtaaagtt ttgtaaaaaa ctattaaaag actttcattt
     6121 ttccggtttg aaaaagaaca tttttttttg tctcccgtta tagccgaacg aactgaaacg
     6181 gctgctcccg agccggaata cacactgaat tggaatactg aggagacgat caatgaaaaa
     6241 ctttaccaaa catgcattct caattttggc gctttcagca gtcctgaccc tcccggtttt
     6301 ggctggtgaa aagaaagaat caatcacctt ctcccgcaac atcgtggtga atggaacaac
     6361 catccaggcc ggcacctacg atgtgaaatt caacgaccag accggggaat tcatcattct
     6421 caagcgcggc aaggaacttg ccaaaaccac tggttccctc aaagatcggg gacgcaaagt
     6481 gacctcaacc gaggttttct ttagcaaaca gggcgataat gcagttttaa gcggcgttac
     6541 ttttgctggt gcaaatcaaa cagtggtggt caatgacggc gctcaggccg catcaccaag
     6601 tcagtaatct ctcctcactg tttcgcaaaa cccattctga atgagaatgg gttttgcttt
     6661 tttctctgtg attttttttg gaaataattt caataaactg aacatttttt cttgtattaa
     6721 cctctgtttt caacagaggt ttttcttttt acaaattcat tgagatgacc tggaatcatt
     6781 tgaagcaaag aggtgacagg catcttcaag acttgccagg gagaagggtt tgaccagttt
     6841 tccgacgaac ccgtactggg aatagtcaga cagaattggg tcatttgaat acccgctgca
     6901 cacaatggcc ttcacctgcg ggtcaatcaa ccgcaatttc tccagtgttt cctgcccccc
     6961 catacttccc ggaacagtta aatccaaaat gacgcctgaa aatggggtat ggttttcaat
     7021 tgcctttcca taaagaacaa cggcttcaat cccatctttt gaaacttcta tttcataccc
     7081 cagcgcttct aacatttccc gggtggtttc acgaacgatt tcttcatcgt ccatcaccaa
     7141 gattcgtact ggccgcgatg actcagatga cgcgatttcg ccggtggcca gtcgcaaaga
     7201 cggttgtgcc acctgactgg cgggcagaaa aatatcaaat tgcgtcccaa ttccagcctc
     7261 agacgtcacc gtaatgtatc cgttatgttt tttgacgaca aaataagcaa tcgccaaccc
     7321 cagaccatga ccagtttctt tggttgaaaa atacgggtca aaaattttgg ttaagtgttc
     7381 aggattgacc ccgattccct ggtcaatgac ggatatcttg atgtaatcgc ctggggtgag
     7441 ggggtattga ccagtctcag ccagggtgat gtttttaccg cacaccagca attcaccgcc
     7501 aaggggcatg gcgtcacgag cattgagtgc caggttctgg aaaacctgtc ccatttggcc
     7561 gggatcaatt tcggtgagcc agaggtgtgg gtcaaattga aacaccgccc ggatatttga
     7621 accatgcaat gaaaaaacaa tcgtgtcgcg gataaagtac tctaaattcg caatttccct
     7681 gaccgggacc cctcctttgg caaatgtcag taactgccgg gccagcccct tggctcgatc
     7741 actggcccgc tggacggcgt cgagccgggt gaggattttt tggggattgg taacataggt
     7801 tctcaccagt tcgacatttc caataatggc cgtg
//
LOCUS       DAOQDO010000020         7602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5485_length_7602_cov_3.201537, whole genome shotgun sequence.
ACCESSION   DAOQDO010000020 DAOQDO010000000
VERSION     DAOQDO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5485_length_7602_cov_3.201537"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PKZ53_00730"
     CDS             complement(<1..974)
                     /locus_tag="PKZ53_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ38986.1"
                     /translation="MNRPKVLIVDDDPQNLTILVDLFEHYHAHFEVYQALNGNLALRI
                     AQARIPDVIITDWEMPEMDGIELIRQLKASSKTAEIPVIMCTGVMTSSINLKTALEAG
                     AVDYIRKPVDPIELEARVGTVLKLADSLRTIRKKNQELEAAHLQADRIFSAFAQALPG
                     KILDRKYRLEEKIGAGGFGVVFRGTHLQLNRPIAIKIFKPYPGNDSPKAIERFRREGI
                     SATRLNHPNAIQILDSGISSDGILYLIMELLHGVSLRQALQEAGKLSPIRCREIILPV
                     CDVLAHVHELGMVHRDIKPDNVFLHQSPGGEVVKVLDFGIAKLLADET"
     assembly_gap    1165..1264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1467..6800)
                     /locus_tag="PKZ53_00735"
     CDS             complement(1467..6800)
                     /locus_tag="PKZ53_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ38987.1"
                     /translation="MISLSAYTLNSVLHETENSIVFRGTRAQDGLPIIGKMIRDEYPT
                     TAQLARFQGEFELLRNLAGDGVVEAFAIEQFENRKVILLEDFGGRSLHQLYHHEPCPV
                     VDGLRLAIRISRILSRIHQANVIHKDITPGNIVFNPAKDIIKLIDFGSATHFALEKSE
                     FQSVNVLEGTLAYISPEQTGRMNRGLDYRTDLYSLGVVLYELFTGQLPFQSRDLLELV
                     HCHLAVDPIPPHQVNPGIPEIVSDIILKLMAKAAEDRYQSAEGVTFDLHETLNQLEHT
                     GRVSSFVLGRHEVLNRFQIPQKLYGRETETAELLSLFRRVSDGTSELGLISGYSGIGK
                     SALVQELFKPITEERGYFISGKFNQFQRNVPYASVIQAFQSLIRQILTESESQIAQWR
                     QELTEALGINGELITAVIPEVELIIGPQPPVVSLGPTETRNRFNLVFQNFIRVFAQAD
                     HPLVLFLDDLQWADPDSLKLIGTLISANDIQHLLVLGAYRDNEVDPGHPLMMMLHEAQ
                     KNSAQITDIKLTPLGVQQVTALVADTLNQSPETVAPLAELVFGKAAGNPFFTSEFLKH
                     LNQDRLIWFDPEPAEWVWEIDQIRTAQITDNVVELITDNIQRLGPATVEVLTMAACLG
                     NQFDLLKLSLVTNKTERKIISDLREAVREGLILPLGDGYQVIEYGILADRTSETIECK
                     FAHDRIQQAVYSLIPSASLEKIHFTVGHRLLNGVASDQVEDLLFEIVNHLNAGRRLLS
                     TDHERLDLAQLNHRAGCRARESAAFQPALNYFSTAVELLPADVWSAQYQFTLKLYEDA
                     CEAAFLCGQYEQMQTLAAEVMTNASNVIDTLKIYDVRSQALFAQNQLKDSVDIALEFL
                     KKLEIEFPLQPTQDDIGRALHELNRLLNGRPIESLVDLPVMTDPLALAAQQMLARIGV
                     AAYAAYPQTFPLIVAHRVKLSLLHGNTPLAVSAYASYGLFLSGWLQNIDAGYEFSKLA
                     LELIEKFGAEHLRIRVSLIHYAMVSYWKDHLKDCMAPLIEIYKSGLEQGDFEYATSLL
                     LMIALCEINLGTELNLQYQNLVKYGDLLKRMKQTRPGVAISILKQVVSNLLGQGTEPI
                     VLIGDDCNATHLLANPAIQKDTALLGVIHLYQLELCIYFQRYAEALVQADLTRRYLPG
                     LNSTGSIPMYHFYDALARLAIAKTDGEQRAALLAQVEATQLKLGLWARKCPGNVAHKF
                     HLVEAERLGLEGKIPEAMAAYDTALELAKRNGYIQEEALANELAAGFYASLGRTKIAR
                     MYLEEARYGYARWGATAVLQRLEANHPELLSRPAEIRGPATGKFKSPTVTTSTRTDST
                     STGAIDMNTVIRASQVISSEIVLDRLLVKIMRLVIGNAGAQRAALLLPKDGQFFIEAQ
                     CDGDLDHIQVLQSQPVTASTSLPQTVINFVARTLELVLSDNVSEDRRFARDPYIEREH
                     PASMLCLPVQNQGKLTAILYLENKSVPGAFTSQRVELMNLLTAQSAMSIENALLYASL
                     ESKVKVRTAELTITVEQLQRSEAEIKEKNTQILDSIRYAERIQQAILSADEPVVQSLG
                     EYFILFRPRDIVSGDFYWFQEVDGALLLAVADCTGHGVPGALMSMIGTSLLNQIVIEK
                     QIHTPGLILQHLHVGIRRALRQDEGEASSQDGMDIGIIRIEADRQRVTFAGAKRPLYL
                     ADSTGSFQEIQGDRKPIGGRQKEAVRHFTEHQVEIQDEMTFYLCTDGLADQPGDIESK
                     FGTRRVRELLQANCQLSLDEQHTRIIAAIAAHQGQTPQRDDMALVGVRIRI"
     gene            7017..7454
                     /locus_tag="PKZ53_00740"
     CDS             7017..7454
                     /locus_tag="PKZ53_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38988.1"
                     /translation="MTTFKDLDPIETDFGIPFIPVGWLEKDSVFETGPVSQEFFTKLE
                     ALVVHPWQPFHRLGVHFCELCQFDPPGFNAEVYIPFEGKLYMAPVGIKHYIAAHWYRP
                     PQIFIEAVLACPPMRSMAYHKSLLENGARPLIKALANPPQMKP"
BASE COUNT         1829 a         1935 c         1898 g         1840 t
ORIGIN      
        1 ttggtttcat ccgccagcaa cttggcaatc ccaaaatcca gcaccttgac aacctcgcca
       61 cccggtgact gatggagaaa cacattgtca ggtttgatgt cacggtggac cattcccaat
      121 tcatggacgt gggccagcac atcacacacc ggaaggataa tctcacgaca ccgaattggg
      181 gacaattttc cggcttcctg cagcgcctga cgaagtgaaa ctccgtgtaa caactccata
      241 atcaggtaca ggatgccgtc acttgaaatg cctgaatcca atatctgaat ggcgttgggg
      301 tggttgagtc gggtggctga aatcccttcg cggcgaaacc gctcaatggc tttgggtgaa
      361 tcattgcctg ggtagggctt gaaaattttg atggcaatcg ggcgattgag ctggaggtgt
      421 gttccgcgaa acaccacacc aaatcctccg gcaccaattt tttcttcaag ccggtatttc
      481 cgatccagta ttttgccggg cagtgcctgg gcaaaagccg aaaaaatccg atctgcctgt
      541 aaatgagccg cttcgagttc ctgattcttt ttgcgaatgg tccgcagcga atccgccagt
      601 ttgagcactg ttccgacacg agcttcaagt tcaatcggat caaccggttt gcgaatgtaa
      661 tcaaccgcac cggcttccag tgctgtcttg aggttgatgg atgaagtcat cacgccagtg
      721 cacataatca ccggaatttc agccgtttta gaactggctt ttaattgccg aatcaattca
      781 atcccatcca tttccggcat ttcccaatct gtgatgatga cgtcagggat tcgcgcctgg
      841 gcaattcgga gcgccagatt cccatttaac gcctgataca cttcaaaatg ggcgtggtaa
      901 tgctcaaaca aatccaccag gatggtgagg ttttgcggat cgtcatccac aatcagtact
      961 tttgggcggt tcatgaacct tcctctgaaa gtcaggccga agaattcggg ctgaagaatt
     1021 cgggcttggg gcggaagacc ctgggctgga aaagcatttc aatcttcttg ttttcaaact
     1081 ctaagccctg ttgccaatgt cttcaaccct cagcccgact tcttcagccc aggttagggt
     1141 ttggggcttg gggctgaaga ctcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnacttct tcagccccaa accctgagcc ctggtttttt cagcccgact tcttcagccc
     1321 aggttagggt ttggggcttg gggctgaaga ctcgcaagct cggggctgaa gaaaacgggc
     1381 tccgggtatt gaacccaact tcttcaaccc gacttcttca gccccaaacc ctgagccctg
     1441 gttttttcag cccgacttct tcagccttaa atcctgattc gcactcccac caatgccatg
     1501 tcatcccgct ggggagtttg gccttgatgg gcagcaatgg cggcaatgat acgggtgtgt
     1561 tgctcatcca gcgacaactg acaattggct tgcagcaatt ccctgacccg ccgggttcca
     1621 aatttcgatt cgatgtctcc gggttgatcg gccagaccat cggtgcacag ataaaacgtc
     1681 atttcatcct gaatctcaac ctgatgttcg gtaaagtggc gaacggcttc tttctggcgg
     1741 cctccgattg gcttccggtc accctggatt tcctggaaac tccctgtgga atcagccaga
     1801 tagagtgggc gtttggcgcc agcaaaagtc acacgttggc ggtcggcttc gatgcgaata
     1861 atgccaatgt ccattccatc ctgtgaactt gcctcgcctt catcctgacg cagcgcccgg
     1921 cggataccaa cgtgcaggtg ttggaggatc aaccccggcg tgtgaatctg tttttcgatc
     1981 acaatttgat tgagcaggct ggtgccaatc atagacatca acgcgcctgg tacgccatgc
     2041 ccggtacaat cggccacggc cagaagcaaa gcgccatcaa cttcctgaaa ccagtagaaa
     2101 tcacccgaca caatatcgcg cggacgaaac aggatgaaat attcgcccag ggactgtacc
     2161 acgggttcat cggctgaaag aatcgcctgt tgaatccgct cggcgtagcg aatgctatcc
     2221 agaatttggg tatttttttc tttgatctca gcttccgacc gctggagttg ctcaaccgta
     2281 atggtcaatt cagccgtgcg cactttgact tttgactcca ggctggcata gagcaaggcg
     2341 ttttcaatcg acatggccga ttgcgcggtc agcaggttca tcaattcgac ccgctgtgaa
     2401 gtaaaagcac ctggaaccga tttgttttca agatataaga ttgcggtcag cttgccttga
     2461 ttttgaaccg gaagacagag catcgaggct ggatgttcac gttcgatata gggatctctg
     2521 gcaaaccgcc gatcttcact gacattgtcg gaaagaacca gttccagcgt tcgggccaca
     2581 aagttgatta cggtttgcgg caaggatgtc gaggcggtca ctggctgaga ttgcaacacc
     2641 tggatgtgat ccaaatcacc atcacattgg gcttcaatga aaaactggcc atctttgggc
     2701 aacaacaacg cggcccgctg ggcaccggca ttgccaatca ccagtcgcat aattttgacc
     2761 agcaaccgat ccagcacaat ctcactggaa atgacctgag atgcccgaat gacggtattc
     2821 atgtcaatag ccccggttga cgtgctatcg gttcgagtcg aagtggtcac tgtcggcgat
     2881 ttgaattttc cggtggcagg cccacggatt tcagccggtc ggctcaacag ttcggggtga
     2941 ttcgcttcaa gccgttgcag gacagcggtg gcaccccatc gcgcatagcc atagcgagct
     3001 tcttccaggt acatacgagc aattttggtt cgcccaagac tggcataaaa cccggcggca
     3061 agttcattgg ccagagcttc ttcctgaatg tagccatttc gtttggccag ttcgagcgcg
     3121 gtgtcatagg ccgccatcgc ctctggaatt tttccttcca ggcccagtcg ctcggcttcg
     3181 accagatgaa atttgtgggc aacatttccg gggcattttc gcgcccataa accaagttta
     3241 agctgggttg cctcaacctg tgccagcaat gccgcccgct gttcgccatc agttttggca
     3301 atcgccaacc gggccagggc gtcataaaaa tgatacatag gaattgaacc ggtggagttc
     3361 aatccaggaa gatagcgtct ggtcaagtca gcctgcacca gtgcttcggc atagcgctgg
     3421 aagtagatac ataattcaag ttgatacaaa tggatcactc ccagcagggc ggtatctttc
     3481 tgaatggccg ggttggccag caaatgggtc gcgttacaat catccccaat cagtacgatt
     3541 ggctctgtgc cctgacccaa caggtttgat acgacctgtt tcagaatact gatggcaact
     3601 ccggggcggg tctgtttcat gcgtttgagg aggtcaccat atttgaccag attctggtac
     3661 tgcaagttca attcagtgcc cagattgatt tcgcacaggg caatcatcaa caggagcgac
     3721 gtcgcatatt caaaatcacc ctgttccagg ccgcttttgt agatttcaat cagcggcgcc
     3781 atacagtctt tcaggtggtc tttccagtaa ctcaccatgg cgtagtgaat gagcgaaacc
     3841 cgaatccgga ggtgctcagc cccaaatttt tcaatcaact ctaacgccag cttgctgaat
     3901 tcatacccgg catcaatgtt ttgaagccag ccacttaaaa acaaaccgta agaagcgtag
     3961 gcggatacgg ccagtggagt attgccgtgc agaagcgaga gtttgacccg gtgcgcgaca
     4021 atcaatggaa aggtttgcgg ataggcagca tacgcggcca cgccgatccg ggccagcatc
     4081 tgttgagccg ccagtgccag cgggtcggtc atcaccggca gatcaaccag actttcaatt
     4141 ggccttccgt tgaggagccg attgagttcg tggagggctc gcccgatatc gtcctgggtt
     4201 ggttggagtg gaaattcgat ttccagcttt ttgagaaatt cgagcgcaat gtccactgaa
     4261 tccttgagct ggttttgcgc aaacagggcc tgggatcgca cgtcataaat ctttaaggta
     4321 tcaatcacgt tgctggcgtt ggtcatgact tcggcggcca gcgtctgcat ttgttcatat
     4381 tggccgcaca ggaacgctgc ctcacaggcg tcttcataca acttgagtgt gaactgatac
     4441 tgagcagacc agacgtcggc gggtaacaat tcaacagccg ttgagaaata attaagggct
     4501 ggttgaaagg cggcggattc gcgggctcga cagccagccc gatggtttaa ttgcgccagg
     4561 tccaggcgct cgtggtcagt acttaacaac cggcgcccgg cattgaggtg attgacgatt
     4621 tcaaacaaga ggtcttcgac ctgatccgaa gcaactccat tcaagagccg gtgtccaacg
     4681 gtaaagtgga ttttttccag actggctgat ggaatcaatg aatagaccgc ctgctggatt
     4741 cgatcatggg caaatttaca ttcaatggtt tctgatgtgc ggtcagccag aatgccatat
     4801 tcaatcacct gatagccgtc gcccagtggc aggatcagtc cttcacgaac agcttcgcgc
     4861 aaatcggaaa tgattttgcg ctcagttttg ttggtgacca gcgacaattt cagcaaatcg
     4921 aactggttcc caaggcaggc ggccatcgtg agcacttcga ccgtggcagg acctagccgc
     4981 tggatattgt cggtgatcaa ttcgaccacg ttatctgtaa tctgggcggt ccgaatttgg
     5041 tcaatctccc agacccactc agcaggttcc ggatcaaacc aaatcagccg gtcctgattc
     5101 aggtgcttta aaaactcgct ggtaaaaaat ggatttccag cggctttccc aaaaaccagt
     5161 tccgccagtg gggcaacggt ttccggtgac tgattgagcg tatcggccac cagggcggtg
     5221 acttgctgaa ctccgagtgg agtcagtttg atatccgtaa tctgggcgct gtttttctgg
     5281 gcttcgtgga gcatcatcat caatggatgc cctggatcaa cttcattatc acgataggcc
     5341 ccaagcacca gcaaatgctg gatgtcattg gctgaaatca acgtgccgat caacttcaac
     5401 gaatcagggt cagcccactg cagatcatcc aaaaagagca ccagcggatg atcagcctga
     5461 gcaaataccc gaataaagtt ttgaaacacc agattgaacc ggtttcgggt ttcggttggc
     5521 ccaagtgaca caaccggagg ctgcggccca atgatgagtt caacttcagg aataacagcg
     5581 gtgatcaatt ccccgttgat gccgagggct tcggttaatt cctgacgcca ttgggcaatc
     5641 tgggattccg attccgtcaa aatttgccga atcagcgatt ggaaagcctg aatgaccgag
     5701 gcatagggca cgttgcgctg aaactggtta aatttcccgc tgataaaata gccgcgttct
     5761 tcagtgattg gtttgaataa ttcctgaacc agggctgatt ttccaattcc ggaatagccg
     5821 gaaatcagtc ccagttctga cgttccatca ctcacgcgcc gaaacaggct cagcaattca
     5881 gccgtttcgg tttcgcgtcc atacagtttt tgaggaatct gaaaccggtt gaggacttcg
     5941 tgccgcccga gcacaaacga tgagacccgt ccggtatgct caagctggtt cagcgtttca
     6001 tgcaggtcaa aagtgacgcc ttcggcactt tgataccgat cctctgctgc cttggccatc
     6061 aatttcagaa taatatccga gacaatttcc gggattccag gattcacctg atgcggagga
     6121 atcgggtcaa cggccagatg gcaatgcacc agttccagca agtcgcggga ctgaaacggg
     6181 agctggccag taaagagttc atacaggacc acaccgagcg aatacaaatc tgtccgatag
     6241 tcgagccccc ggttcatacg tccggtttgt tccggcgaaa tataggccag ggttccttca
     6301 agcacattca cgctttgaaa ttccgatttt tccagagcga aatgagtcgc actgccaaaa
     6361 tcaatcaatt tgataatgtc tttggctggg ttgaaaacaa tattgcccgg tgtgatgtct
     6421 ttgtgaataa cattggcttg atggatacga ctcaaaatcc ggctgatacg aattgccagt
     6481 cggagaccat ccaccaccgg acagggttcg tgatgatata actggtgcag cgaacggcct
     6541 ccgaaatctt cgagcaaaat cacctttcgg ttttcaaatt gctcaatggc aaaggcttca
     6601 accacaccat cgccagcaag gttacggagc aattcaaact cgccctgaaa ccgtgccagt
     6661 tgagcggtgg ttgggtattc gtctctgatc atcttgccaa tgatgggcaa accgtcctgg
     6721 gcacgagttc ctcgaaagac aatcgaattc tcagtctcgt gcagcactga gttgagcgtg
     6781 taggcactga gcgaaatcat ggatttggtt gttagggtag atggattttc ggatggaaaa
     6841 gcagaacgcc gcaattatga atgatcaatt ctgaaaagac caaccgaatt ggtaagggct
     6901 tgaggcttga ggctcggggc tgaggattca aacttgactc atttttaata tccagagacg
     6961 attccaaagg aaatcaactg gtacaacctg gtctgaaaaa tctctgagaa caaccaatga
     7021 cgacctttaa agatcttgac ccgattgaaa ctgattttgg catcccattt attccagttg
     7081 gctggctcga aaaggattct gtctttgaaa ccggacctgt cagccaggag ttttttacca
     7141 aactcgaagc tcttgtcgtt cacccctggc aaccgtttca ccgtctggga gttcactttt
     7201 gcgaactatg ccagtttgat ccgccgggtt ttaacgctga ggtttatatc ccttttgagg
     7261 ggaaactcta tatggcgcca gtcggaatca aacattacat cgccgctcac tggtaccgtc
     7321 cacctcaaat cttcattgag gcggtactgg cctgtccgcc aatgagaagc atggcctacc
     7381 ataaatcgct gctggaaaac ggtgctcgac cactgatcaa agcactggcc aatcctcccc
     7441 aaatgaaacc ctgagaagac caaaaaagga agcttcctgc gttgggaagc ttcctttttg
     7501 gtcaggtaac tgattggaag gactctttcg caaacccgga acccggaacc cggaacccgg
     7561 ttctatttct tctccggctt tggcttataa atgccgtcaa ag
//
LOCUS       DAOQDO010000021         7587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5503_length_7587_cov_2.911445, whole genome shotgun sequence.
ACCESSION   DAOQDO010000021 DAOQDO010000000
VERSION     DAOQDO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5503_length_7587_cov_2.911445"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1753
                     /locus_tag="PKZ53_00745"
     CDS             158..1753
                     /locus_tag="PKZ53_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNJ38989.1"
                     /translation="MNLVKFDTWKEDLSSGFLVFLIALPLCLGISIASGFPPVAGILT
                     AIVGGILSTFLGSARLTIKGPAAGLIVIALGAVTELSYGDPVAGYKRALAVGVVAGVL
                     QIILALIGAGTIGQLMPSSVVHGMLAAIGVIIISKQAHTLLGVTPQGKEPLHLLAEIP
                     QSIAHLNPEVLLIGLLSFMILVTLPLIKSKWAKLIPGPMLVLAVAVPLGMYFNLGTEH
                     TYSMAGHVYQTGPKFLIRLPGSLLSALSFPDFSMITSGPSIKYIIMFTLVGSIESLLS
                     VIAVDSLDPEKRTSDLNRDLLATGIGNTIAACIGGLPMISEIVRSKANIDNGARSSLS
                     NFFHGLFLLLSVALIPGMLQKIPMAALAAMLIYTGLRLASPSEFKHAYHIGPEQLFLF
                     VVTMVVTLTTDLLVGVGVGRVLKVILHLVNGAPLGSLFRAPLKESQEGTTLHLEIRDA
                     AIFTNYLGLSNKLNSLDERIKRVVINFENTWVVDHTVLEKLHSTASRWKDRELLLTGL
                     DKHTPMSNHQLAARRRTREGALA"
     gene            1750..4680
                     /locus_tag="PKZ53_00750"
     CDS             1750..4680
                     /locus_tag="PKZ53_00750"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNJ38990.1"
                     /translation="MSQARHSWAVADIGLDLKQAIDHAAHYLPAQGPIGVFIHHNTLH
                     AFQHLPFETAVREAMHTFGTRPYLTEAQYQAERRRGRIVEADIDAVLADEPEVVIFPR
                     MLTRRQLRRAILVAGLRPLSPQNVEWQIDECGLMERLRPDLDDQIRQNILEGNDESVA
                     ARTLFAACFQRISVLQPEVSKGYSRPRDRVLDVTGVDIDEVIHPWLIRFCSVFLDQGM
                     AYWPMPKRERGLYQAVRDLMRQPLIIEPARLSGLRQRFEAQARAGLTATDVVLRHLDL
                     FGLDTKGWEAYLTAELLALSGWAGLIRRLEEDPGLAPHIQIPCTLTDFLAVRLTMTAV
                     AVDNLWREHNLSGNIWAEWRVKPQKPAVSETEHMAAVARFFDVAQLLGLSAKHLLSLS
                     HKEFQRLREEVTLFDELERCRILHLAYEKRHEALILQPLSTHRRDITVKRTASRPAAQ
                     VFFCIDEREESMRRALEELAPEVETFGAAGFYGVAVNFQGLDDPHGVALCPVVVKPQH
                     AVLEKPREDDQPASVRRQNLRRLWSNLAHTSYIGSRTLIRGWIGTSGLGLVSIFPLIT
                     RVLAPRRAGQIRHWLSQQFLPQPRTRLTLVRTGDTERVHDLLLGFSPQEQAERVAGVL
                     KPAGLVANHARLVLVLGHGSTSLNNPHESAHDCGACGGRRGGPNARLFAAMANHPDVR
                     ARLRHLGVEIPADTWFVGGYHDTCNDDVVFFDQEEIPASHRAEFARMEQTLDQARAAN
                     ALERARRFEAAHAVVSPREALSHVQERSEHLAEPRPEYGHATNAVCVVGRRSLTRSLF
                     LDRRAFLVSYDPDLDSDEQLLTAVLGAAGPVCAGINLEYYFSFVDNERYGCGTKLPHN
                     VTGLVGVMNGHASDLRTGLPWQMVEIHEPVRLLLIVETSPHRLMKAVNRSAVVSELVK
                     NRWIRLATIDPDDGHIEVYRDGRFEPFSEQPVPLPEVSTSVAWYSNKLEHLPVARINQ
                     KLAK"
     gene            4723..6096
                     /locus_tag="PKZ53_00755"
     CDS             4723..6096
                     /locus_tag="PKZ53_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ38991.1"
                     /translation="MLIVLLPGCAFATLGLTWLFGMEMSERLVARLTATCYSLVTLVA
                     GGLWAAMFVQGQSSVTTSLGDWFEIGDYHYQLVLMVDRLSMPLVALSVVLVGLVGIFS
                     VRYLHRDAGFYRFFLLLHLFGFGILLLFTAGSFDLLIGGWELVGLTSVLLIAFFQHRN
                     EPVTSALRVFATYRACDIGLLVAVVLMHHLAGSTTYATLFPAGWPNGGGVIAGSSATI
                     LGVLLLVASLGKAAQVPFSGWLPRAMEGPTPSSAVFYGALSVHAGAYLLLRAEPILKA
                     SPGVALAVVVVGILTAIHATMVGRACSDAKTSLAYASMAQLGVIFVEIGCGWSWLAIF
                     HITGHAVVRTLQFLRAPSMLHDFHQVHAAAGGHLGKTGAHYEQLFPEYIQTWLYRLAL
                     DRGHHDTVLDRYVVAPLLALANQLYRLEEGWSVMAFGPTGEYREWLITKVGLLSLQKK
                     EEDISVH"
     gene            6086..>7587
                     /locus_tag="PKZ53_00760"
     CDS             6086..>7587
                     /locus_tag="PKZ53_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ38992.1"
                     /translation="MFTKFMFPWISLSQLLLVIFSIVTWHIITADKARKAAIVGLGLN
                     VLFLSAAFGEVILARGQTLTEPWPVEGVRSLFVMDSLNALPMMVLSALALGMVILVPR
                     RKLTPRWLAGVLLLTAATLTAYAANTLILLVLAWGLAVLPFLVGRFFSQLNESQLPAL
                     SKLAMIGSVASLAIGVGMIISCKPELAFQPWIEPSSTLTGITPFLRYAFVFLVVAVFL
                     RQGLVPVHSWVVTAYERGPLLPLTLFLNGHLGAFLLVRLVNPLLPDIAREALPILGYL
                     GLFTAAYTAILALAEPKPRRLLALLTVSQTSFVLLGLQSSTVDGMVGSFIHWQVVAVA
                     TTVLAAVYVGIEARLGSAIEGKNFLGLATAAPRLAVFFVVAGLALVGLPLTLGFCAED
                     LLLHATLATHPQLGIIMPLVTAFNAFSVLRLFARLFLGRPIVEAELLVDALPRERLVL
                     TCCLLFLILGGLFPSVLLQFPSTSARRLSMPATTVLNRTTGDSNTSGSGF"
BASE COUNT         1455 a         2094 c         2151 g         1887 t
ORIGIN      
        1 gtatttccga aactgaaatg gggctagctt acctggatga gccgtcgttg cctatcccta
       61 aaaattggtc ggttttctcc cttatcaagg ggatttcgca ttgttgccct ttgacgcatc
      121 ctcaccgcgc atttttttga aggagcaaaa ccgaactatg aacctggtca agtttgatac
      181 ctggaaagaa gatctttcct ctggatttct ggtctttttg atcgccctcc cactttgcct
      241 gggaatctca attgccagcg ggttcccgcc cgtggccggg attctgacgg cgattgtcgg
      301 cggcattctt tcaacttttt tgggaagtgc ccggttaacc atcaagggcc cggcggcggg
      361 cctcatcgtc attgcgctcg gcgccgtgac cgaactgagt tacggcgacc ccgtggccgg
      421 gtataaacgc gccctggccg ttggcgtggt ggctggcgtg ctgcagatta tcctggcgct
      481 gatcggggcc ggaaccattg gccaactgat gccatcttcg gttgttcacg ggatgctggc
      541 ggcgattggg gtgatcatta tctccaaaca ggcccacacg ctgctgggcg tgaccccaca
      601 gggcaaagaa ccactccact tactggcaga aatcccccag agcatcgccc atctcaaccc
      661 tgaagtcctg ctgattggcc tgctgtcgtt tatgattctg gttacgcttc cgctgatcaa
      721 gtcaaaatgg gccaaactca tcccagggcc aatgctggtc ctggccgtcg ccgtaccact
      781 cgggatgtat ttcaatttgg gcaccgagca cacctattcg atggcgggtc atgtctatca
      841 aaccggtcca aagttcctga ttcgccttcc aggttcactc ttgagtgccc tctcgtttcc
      901 tgatttctcg atgatcacca gtggaccgtc aatcaaatac atcattatgt tcacgctggt
      961 tggttcgatt gaatcgttgc tcagcgtgat tgcggttgac tcgctcgacc cggaaaaacg
     1021 caccagtgac ctgaatcggg atttgctggc aaccggcatc ggcaacacga ttgccgcctg
     1081 tattggtggt ctcccgatga tttcggaaat cgtccgcagc aaagccaaca ttgacaacgg
     1141 tgcccggagt tcgctctcaa acttctttca tggccttttt ctgttgttgt cggtggcatt
     1201 gattccagga atgctccaaa aaatcccgat ggcagcactg gcggcgatgc tcatttatac
     1261 agggctacgg ctggcatctc catccgagtt taagcacgcc tatcacattg gaccggaaca
     1321 gttgttcctg tttgtcgtca ccatggtggt gacattgacc actgacctgc tggttggggt
     1381 tggggttggg cgggtcttaa aagtgattct gcatctggtc aacggtgcgc cactgggcag
     1441 tttgtttcgg gccccgctca aggaaagcca ggagggaacg acactccacc tggaaatccg
     1501 tgatgcggct atctttacca actatctggg gctaagcaat aagctcaact cgctcgacga
     1561 acgcatcaaa cgcgtggtga tcaatttcga gaatacctgg gttgtggacc acaccgttct
     1621 ggaaaaactg cacagtacag ccagccgctg gaaagatcgt gaattattgc tcaccgggtt
     1681 ggataagcat acaccaatgt cgaaccatca actggcggca cgccgccgga cacgggaggg
     1741 cgcgctcgca tgagtcaggc acgacacagt tgggcggtgg cagacatcgg tctcgatctc
     1801 aaacaggcga ttgaccatgc ggcacattat ttaccggctc aaggaccaat cggtgttttt
     1861 atccaccaca acaccttaca cgcctttcaa cacctgccct ttgaaacagc ggtgcgcgaa
     1921 gccatgcaca cctttggcac ccggccttat ttgaccgaag cccagtatca ggccgagcgc
     1981 cgacggggac gcattgtgga ggctgacatt gatgctgtgc tggcagatga accggaggtc
     2041 gtcatctttc ctcgaatgct cacccggcgg caattgcggc gcgcgatcct ggttgccggg
     2101 ttgcgcccgc tgtcaccgca aaacgtagag tggcaaattg atgaatgtgg cctgatggaa
     2161 cgccttcgcc cggatttaga tgatcagatc cgtcagaata ttttggaagg caatgacgaa
     2221 agcgttgctg cccgaacact ttttgccgcc tgttttcagc ggatttcggt gctgcaacct
     2281 gaagtgtcaa aaggatatag ccgtccgcga gacagagttc tggatgtcac cggggttgat
     2341 attgacgagg tgatccatcc gtggctgatt cggttctgtt cggtctttct ggatcaagga
     2401 atggcgtact ggccgatgcc gaaacgggaa cggggcctgt atcaggctgt tcgtgacttg
     2461 atgcgccagc ctctgatcat tgaaccggcc cggctgagtg gtcttcggca acggtttgag
     2521 gcgcaggcca gggcgggcct gaccgccacg gatgtggtgc tgcgccatct ggatcttttc
     2581 gggctggata caaaaggctg ggaagcgtat ctcacggctg aactgctggc actttctggt
     2641 tgggccgggt tgatccgacg actggaggaa gacccaggac tggcgcctca tattcaaatt
     2701 ccctgcacgt tgaccgattt tctggccgtt cggctgacaa tgacagccgt ggcggtagat
     2761 aacctgtggc gtgagcacaa cttgagtgga aatatctggg ctgaatggcg agtcaaaccc
     2821 caaaaaccag ccgtctcaga aactgaacac atggcagccg tggcccgatt ttttgatgtt
     2881 gcccagttac ttgggttatc ggccaaacat ctgttgtcac tcagtcacaa agagtttcag
     2941 cgactgcgcg aagaagtcac gctctttgac gaactcgaac gctgtcgaat cctccatctg
     3001 gcgtatgaaa aacgccacga agcactgatc ctccagcccc tgtccaccca tcggcgggac
     3061 atcactgtca aacgtacagc cagccgccca gccgcccagg tatttttttg cattgatgaa
     3121 cgcgaagaat ccatgcgccg ggccctggag gaactggcac cagaagttga aacatttggc
     3181 gcggcagggt tttatggcgt cgcagtcaac ttccagggac tggacgatcc ccacggcgtg
     3241 gccctgtgtc cggttgtggt caaaccgcag catgctgttt tagagaaacc tcgcgaagac
     3301 gatcaaccag cctctgtccg gcgccagaat ctacgccgac tctggtccaa tctggcacac
     3361 accagctata tcggctctcg aacgctcatc cggggctgga ttggcaccag cgggctcgga
     3421 ctggtctcaa tttttccatt gatcaccagg gttttggcac cacgccgcgc cgggcaaatt
     3481 cgccactggt tgagccagca gttccttccg caaccgcgca ccagactgac actggtacgc
     3541 accggtgata ccgaaagagt gcatgacctg ctcctggggt tttctccaca agaacaggcc
     3601 gaacgagtcg ctggtgtcct gaaaccggct ggactggtcg ccaatcacgc ccgactggta
     3661 ctggtgctgg gtcacggttc gaccagtctc aacaatcctc acgaatcagc gcacgattgc
     3721 ggcgcctgtg gtggtcggcg cggggggcca aatgcgcgac tctttgcggc catggccaat
     3781 catccggacg tccgggcccg gctacggcac ctgggggttg agatcccggc tgatacctgg
     3841 tttgtcggtg ggtatcacga tacctgcaat gatgatgtgg tcttttttga tcaggaggaa
     3901 atcccggcca gtcaccgggc tgaatttgcc cggatggaac aaacgctgga tcaggcccgg
     3961 gctgccaatg cactggaacg agcccggcga tttgaggccg cacatgcggt tgtgtcgccg
     4021 cgtgaagcgc tctctcacgt ccaggaacgg tcagaacacc tcgccgaacc gcgccccgag
     4081 tatggccacg cgaccaatgc cgtgtgtgtg gtgggccggc ggagcctgac gcgttctctc
     4141 tttctggacc gacgcgcctt tctggtttct tatgatccgg atctggattc tgatgaacaa
     4201 ctgctcacgg ctgtgctcgg tgccgccggg ccggtttgcg ctggaatcaa cctggagtac
     4261 tacttttcat ttgttgataa cgaacgctat ggatgcggca ccaaattgcc ccacaacgtc
     4321 accgggctgg tcggcgtgat gaatggccac gccagcgacc tgcggaccgg attgccctgg
     4381 cagatggttg aaattcacga gccagtcaga ttgttgctca ttgtcgaaac cagccctcac
     4441 cgactgatga aagccgtgaa ccggagcgcc gtggtctcag aactggtgaa aaatcgctgg
     4501 atacggctgg cgacaattga ccctgatgac ggacatatcg aagtgtaccg ggatggccgc
     4561 tttgaaccct tttcagagca accggttccg ctgcccgaag tcagcacgtc agttgcctgg
     4621 tattcaaaca agctcgaaca cctgccagtt gcccgaatca accagaagct ggccaaataa
     4681 taaaaatctg gaatgaatgt tatgaacttg agttttttcc ttttgctcat tgttttgttg
     4741 ccggggtgtg cctttgccac actcggactt acctggctgt ttggaatgga gatgtcggaa
     4801 cgactggtcg cccggttgac agccacctgt tattcactgg ttacactggt tgccggcggg
     4861 ctctgggcag cgatgtttgt acagggacag tcaagtgtca cgacatcact gggcgactgg
     4921 tttgaaatcg gggactatca ctatcaactc gtgttgatgg ttgatcggct ctcaatgccg
     4981 ctggtggcac tcagtgtcgt gctggttggg ctggttggta ttttttcggt tcggtatttg
     5041 caccgcgatg ccgggtttta ccggtttttt ctgctgttgc acctgtttgg cttcggcatc
     5101 ctgctgctct ttacggctgg ttcatttgat ttgctgattg gggggtggga acttgtgggc
     5161 ctgacttcgg tgcttctgat tgccttcttt cagcaccgga acgagccggt gaccagtgcc
     5221 ttgcgcgttt ttgccaccta tcgagcctgt gacatcggcc tgctggtggc ggttgtcctg
     5281 atgcaccatc tggccgggtc aaccacctat gccacgctgt ttccagccgg ctggccaaat
     5341 ggcggcggcg tgattgctgg cagttcggcc acgatcctcg gggtgttgct gctggttgcc
     5401 agcctgggga aagccgctca ggttccattc tcggggtggc ttcctcgggc aatggaaggc
     5461 ccaactcctt caagcgctgt cttttacggc gcgctttcag tccatgccgg ggcctatctt
     5521 ttgctgcggg ctgagccgat tttgaaagct tcgcctggcg ttgcactggc agtggtagtg
     5581 gttgggatcc tgactgctat tcatgccacg atggtcgggc gggcgtgtag cgatgccaaa
     5641 acgtcgctgg cctacgcgtc aatggctcaa cttggggtaa tttttgtcga gattggctgt
     5701 ggctggtcct ggctggccat ttttcatatc actggccatg ccgtggtgcg gacgctccag
     5761 tttctgcggg caccgtccat gttgcacgac tttcatcagg ttcatgctgc ggcaggtggg
     5821 catctcggca agaccggggc ccattacgaa cagcttttcc ccgaatacat tcaaacctgg
     5881 ctctatcggc tggcgctgga tcgtggccat cacgataccg tgctggatcg ctatgtggtg
     5941 gctcccctgc tggcactggc caatcagttg taccggcttg aagaaggctg gagcgtgatg
     6001 gcctttggcc caaccggaga atacagagaa tggctgatca ccaaagttgg tctcctttcc
     6061 ctgcaaaaga aagaggagga catcagtgtt cactaagttt atgtttccct ggatcagctt
     6121 gagccagctt cttttggtca tcttttccat tgtcacctgg cacatcatca cggcggacaa
     6181 agcgagaaaa gcggcgattg tcgggttagg gctcaatgtg cttttcctga gcgctgcgtt
     6241 tggtgaagtc atcctcgccc gaggccagac tttgaccgaa ccctggccag tggaaggggt
     6301 tcggtccctg tttgtcatgg attcgctcaa tgcgcttcca atgatggtgt tgagtgcgct
     6361 ggcacttgga atggtgattt tggttcccag gcgaaagctg acgccccgct ggctggctgg
     6421 cgtgttgctg ctcaccgccg ccaccctgac cgcctatgct gcaaataccc tgattttgct
     6481 ggtcctggcc tgggggctgg cggtgctccc gtttctggtc ggtcgcttct tcagccagtt
     6541 aaacgaaagc cagcttccgg ctctctccaa actggcgatg atcgggagcg ttgccagtct
     6601 ggctatcggg gtcggtatga tcatttcctg caaaccagaa ctggcattcc agccctggat
     6661 tgaaccctcc agcaccctca cgggcatcac gcctttcctg cgatatgcgt ttgtgtttct
     6721 ggtggttgcg gtttttctcc gccagggatt ggttccagtt cattcgtggg tggtgacagc
     6781 ctatgaacgt ggtcccctgc tcccacttac cctgttttta aacggccacc tcggcgcgtt
     6841 tcttcttgtc cggcttgtca acccgttgct tccagatatt gccagagaag cattgcccat
     6901 cctggggtac ctggggttat tcaccgctgc gtacacggcc attctggcac tggccgaacc
     6961 taaaccacgc cgactcctgg ccttgctcac ggtcagtcaa acctcgtttg tactgctcgg
     7021 actgcaaagc agcaccgttg atggtatggt cggctcattt attcactggc aggtggtggc
     7081 ggtggcaacg acagttttag ccgccgtgta tgtcgggatt gaagcccgat tgggttcagc
     7141 cattgaagga aaaaatttcc tggggctggc aaccgcagcc ccccggctgg ccgtcttttt
     7201 tgtggttgcc gggctggccc tggttggact tccgttgacg cttggatttt gtgcggaaga
     7261 cctgttgctc catgcgacgc tggccacgca tcctcagctt ggcattatca tgccgctggt
     7321 cacggcgttc aacgccttta gtgtcctgcg gctgtttgcc aggctctttc ttggtcgccc
     7381 aatcgttgag gctgaactgc tggttgatgc actgcctcgc gagcgactgg ttctcacctg
     7441 ttgtttactc tttttaattt tgggtggcct gtttcccagt gtactgcttc agtttccatc
     7501 aacttcagcc agacggcttt caatgccggc aaccactgtt ctaaatcgaa ccacagggga
     7561 ctcgaacaca tcgggttcag ggttcag
//
LOCUS       DAOQDO010000022         7559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5536_length_7559_cov_3.377532, whole genome shotgun sequence.
ACCESSION   DAOQDO010000022 DAOQDO010000000
VERSION     DAOQDO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5536_length_7559_cov_3.377532"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="PKZ53_00765"
     CDS             <1..2057
                     /locus_tag="PKZ53_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ38993.1"
                     /translation="RTQVLRVYRPEDGLVSNSVYCLTSVKKTLWIGTRQGISAFDGQQ
                     FRNFTVKDGLISNDISRTGSLVVDDGSIWFGTNEGAVHCRPDREQRLPVPPRLHINSI
                     HVQGQMVGNAINVTFYRTQLPPPLRYDQNSITFEYVGLSFTNEAAVRYVVKLEGFDPE
                     WLPPSTQRFARYTNLPPRRYRFLVKAQSSTGIWSDQQVVEIVIEPPFWQTWWFRFLVV
                     AGVVGIGSSVYAWRIQTLEHRHQQKLASLRQLLESIRIINSQLALSTVLQNIAEESAR
                     LVGGQPGGIGLVEQQQVVFRQLWSDHGWQDTHLVFQFGQGVAGQVVASGTPLVVAHPE
                     GSPDTAYPETVADLYKSGVLAVPIRTRTGLVVGVLEVRNRPGHKLFSETDCQLIESLA
                     NQAAVAIENAGLYGEVAVKNTELEEKNQMIGQSLAELERLYQQEQEVTRTLQELNQMK
                     TNFIVVTSHEMRTPLTVLKGYNEALLGEFFGELSPMQQKSLNVSQRMIDRMVNSFNDI
                     LEMLKINEGVATLHREPTDLTRLTNGVLEELSPFIEKRKQRVDLETSGQLMVAVDQEK
                     LALVLMNLVQNAIKFTPDGGVIHIALKEQENQILLQVTDSGIGIDAGELERIFDKFYT
                     TPDPSTHSSGRFEFSARGTGLGLAIAKSYVEAHGGRIWAESPGKGQGSTFRVELPVQV
                     TE"
     gene            2237..3658
                     /locus_tag="PKZ53_00770"
     CDS             2237..3658
                     /locus_tag="PKZ53_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNJ38994.1"
                     /translation="MTILIIGAGFSGTLTAIHLLRRGFSRPTRIILVSRVEPMARGVA
                     YGTRNDQHLLNVVAGRMSALTDDEDSFLRFAQSHNPSIDGASFVSRRIYGDYLGHLLD
                     RAEAEAAPGMRLERVLSEVESIEVSESNQIATVLLSNGEQITADRVVLATGNFAPSNP
                     PIQNPEFYASPRYVRDPWAPGALDTVDPDAGVLLIGTGLTMLDVVLDLHRRAKLGRAK
                     LVALSRRGLLPQPHRSPGVPPSHDHLPPGLLTGPNTVRNYFHAVRSHIKELAEQNIDW
                     REVINSMRPITPKLWQMLPVKERSRFLRHVRHYWDVCRHRMATEPADILHRLMEDGEL
                     SVVAGRLVSLTEQADSVEVTLRLRQQHDLQRLKVSSVVNCTGPETNIARLADPLMQSL
                     CVQGVIQPDALGLGLELSDKGAAINQNGQASQVLYYVGPLLKARYWESIAVPELRIHT
                     ATLAETIRNSFEAAPAGVTALSE"
     gene            3748..7518
                     /locus_tag="PKZ53_00775"
     CDS             3748..7518
                     /locus_tag="PKZ53_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ38995.1"
                     /translation="MWLHTSFFLQSEKQHSATSGDWRRRCGVICLRLVFSLVVLFGVE
                     FPASSQIYRFKSYTSSSGLPSNVIYSMFQDSRGFLWIGTDSGVCCYDGAGFQIYSKAQ
                     GLPDTNVRAMCEDKQGQLWVTTEGGVSRFTGNGFETFTTAQGLSNNRTYSATVGRDGT
                     LWFGTANGLNRFDGKRFTTYGTQQGLPAERIWALLEDSKGTLWIGQRGGGLIRFQDGV
                     FTTFNQSNGLPDDRVFGLAEDPAGGLWITTSGGLCFFNGSTFRIYTQADGLGSNSTDK
                     VFIDRYHRVWCSTFGGGLCRFENGQFVVFNRAHGLLDNYLTALLQDREGNIWCGTRSS
                     GVCRLSSELFASYTSATGITNGTLTGVAQTPDGTLWFSSLGGGLSSLSKAGEIRLYGL
                     KEGLPDLDLWTVLADSQGRIWMGGARGIACLQNGRIASYSREQIGARDRITAIIEDRQ
                     GRLWFGSHPSTSNGVVMFDGTKFQLFTTEQGLPQNPVSSFTLDADGTLLACSEGGLSQ
                     FDGVRFVPHPVQALLEDRRIRCVYVDEQKILWIGSLNGLFRYDGTRVQHFTRQDGLAS
                     DTVRAIISLRGEIWIGTSGGISVYDGKTFRNYTTREGLLNDEIFTSACLREPDGTLWF
                     GTNDGALRYRAMPEIAVPAVPKVMIRRVLVRDEPQADLAQLQLPYNQNTVQFDFVGVS
                     LVDEDAVVYRHQLEGFERGDWSEATRERSIRFTNLPPGQYVFRVQAGLHKKQWGDTMA
                     LELTVQRPFWQTWWFGLVILASGSGIAFSLYRRQIRQLERRETRKVALLRHIQEQRLA
                     SLRELLESIRTINSQLDVNIVLQHLAEEGARLVNGEPGGIGLVENGQVVFRRLWLGGR
                     WEEYQLRFQLGEAVAGKVAETGKAVIVNDPTASPEIVFPELIQMYYVNGWIDVPIVSR
                     TGKIVGVLDVRRGKDQPLFGESDRQLIESLANQAAIAIENAGLYSELEEKNLQLEEKN
                     LTIAESFGELERLYKKEQEITQTLHELNRMKTNFMIVTSHEMRTPLTVLKGYIEALVE
                     EYLGPLTFTQRRSLDTCQRMVERMVVTFDDILEMLKINEGRIALRPNQFDLTEIVQKI
                     VGDLQTFVERRKQQVFFDAPDSVVLSADQGKIQMVLINVIQNAIKFTHDGGTIHITLT
                     QENDWAKISIADSGIGISAEEVGRIFEKFYTSQDPSTHTSGKFEFSARGTGLGLAIAK
                     SYVDAHGGRIWAESEGTGKGTTVFIHLPFASKSSHVELPKTGKFNVIQKDDS"
BASE COUNT         1749 a         1868 c         2102 g         1840 t
ORIGIN      
        1 accggacaca ggtcttgcgg gtgtatcgcc cggaagatgg gctggtatcc aacagtgttt
       61 attgcttaac cagcgttaaa aaaaccctct ggattggaac tcgccagggc atatcggcat
      121 ttgacggcca gcagtttcgc aacttcacgg tcaaggacgg tctgatcagc aacgacattt
      181 cccgcaccgg gtctctggtg gttgatgatg gcagtatctg gtttggcacc aacgaaggtg
      241 ccgttcattg ccggcctgat cgtgaacaac ggttgccagt cccgccccgg ctccacatca
      301 attccattca tgttcagggg cagatggtgg gcaatgcgat taacgtgacg ttttaccgaa
      361 cacaattgcc gccgccattg cgctatgatc aaaacagtat cacgtttgaa tatgtcggat
      421 tgtcatttac caatgaggcg gctgttcggt atgttgtcaa gcttgaaggg tttgacccgg
      481 aatggctccc accgtcaacc caacggtttg cgcgctatac caatttgcct ccgcgtcggt
      541 atcgctttct ggtcaaggcc caatcatcca ctggaatctg gagcgatcaa caggtggttg
      601 agattgtgat tgaaccacca ttctggcaaa cctggtggtt ccggtttttg gttgtggcag
      661 gtgttgttgg aattggttcc agcgtatatg cttggcggat ccaaaccctc gaacatcgtc
      721 accagcaaaa acttgcttcg cttcgtcagc tcctggaaag tattcggatc atcaattcgc
      781 aactggcact ctcaaccgtg ctccaaaaca ttgctgaaga aagcgcccgt ctggttggtg
      841 gtcaacctgg gggcatcggc ctggtcgaac agcaacaggt tgtgttcagg caattatgga
      901 gcgatcacgg ctggcaggat acacacctgg tttttcaatt tggtcaaggg gtcgcaggtc
      961 aggtggtcgc atctggaacg ccgctcgtgg tggcacatcc tgaaggttca cccgacacgg
     1021 cctatcctga aaccgtggcg gatctttaca aatcaggggt gctggctgtt ccgatccgga
     1081 cgcggaccgg gctggtggtg ggtgttttgg aagtccgcaa ccgaccgggg cacaaactgt
     1141 tttccgaaac tgactgtcag ttgatcgaat ccctggccaa tcaagccgct gtagccatcg
     1201 aaaacgccgg tctctatggg gaagtcgctg tcaaaaacac ggaactcgaa gagaaaaacc
     1261 agatgattgg ccagtcgctg gccgagcttg aacgactcta tcaacaggaa caggaggtca
     1321 cccggaccct gcaggaactc aaccagatga agaccaactt cattgtggtc acctcgcacg
     1381 aaatgcgaac gccgttgacg gtactcaagg gatacaatga agcgttgctg ggtgaattct
     1441 ttggcgaatt gagcccgatg cagcaaaaat cactcaatgt cagccagcgc atgattgacc
     1501 gcatggtcaa tagttttaat gacattctgg aaatgctcaa aatcaacgaa ggcgtggcca
     1561 cactccaccg tgaacccaca gatttgaccc ggctgacaaa tggggtgctt gaggaactgt
     1621 caccattcat tgaaaagcgc aaacagcgag ttgaccttga aacatctggc cagttgatgg
     1681 ttgcggttga tcaggaaaaa ctggcactgg ttttaatgaa tctggttcaa aatgccatca
     1741 agtttacacc tgacggtggt gtcattcaca ttgcactgaa agagcaggaa aaccagattc
     1801 ttcttcaggt taccgactct ggaattggaa ttgacgctgg ggaacttgag cgaatttttg
     1861 ataagtttta caccacaccg gacccttcaa cccacagctc aggccggttt gaattttctg
     1921 cacggggcac cggactcggg ctggcaattg ccaaaagcta tgtcgaagcc cacggtggcc
     1981 gcatctgggc tgagtctcca ggaaaaggcc agggaagcac ctttcgggtt gagcttccgg
     2041 ttcaggtgac agagtgagga ctagggctca gggcttgggg ctgaagaaat tgggctgaag
     2101 aaatcgggct caggacctgg ggctgaagaa tgagtttgat ttcatccctc atccttcatc
     2161 cctcatccct tccgaaaccc cggaacccaa agacttcgcg ctcgccagtt attttcgaaa
     2221 gaggaaatca aaaaacatga ctattcttat tattggcgct ggatttagtg gcacattgac
     2281 ggccatccat ttactccggc gcggatttag ccgcccaact cgcatcatac tggtgagtcg
     2341 agtcgagccg atggctcgtg gcgtggccta tggaacccgc aatgaccagc atttgctcaa
     2401 tgtggtggct ggccggatga gtgctttgac agacgatgaa gacagttttc tgcgctttgc
     2461 ccagagccat aacccatcca ttgacggggc ctcatttgta tcgcggcgga tatatggcga
     2521 ctatctgggg catttgcttg atcgggctga agccgaagcc gcacctggaa tgcggctgga
     2581 gcgtgtttta agtgaggttg aatcaatcga agtttctgaa agcaatcaaa ttgcaacagt
     2641 tcttctgtca aatggggaac agatcaccgc tgaccgggtt gttttagcca ctggcaactt
     2701 cgcaccgtca aatccaccaa ttcaaaaccc agaattttac gccagtcccc gctatgtgcg
     2761 ggatccatgg gcgccgggtg ccctcgatac agttgaccca gacgctggtg tgttgttgat
     2821 tggaaccggg ttgaccatgc tggatgttgt cctggatctg catcgtcgcg ccaaactggg
     2881 ccgtgccaaa ctggttgcgc tctcacggcg cggtttgttg ccgcaaccac accgttcgcc
     2941 gggtgtccct ccgtcgcacg atcatcttcc tccggggtta ctgactggac ccaatacggt
     3001 ccgcaattat ttccatgcgg ttcgatctca tatcaaagaa cttgccgaac agaatattga
     3061 ctggcgtgaa gtcatcaatt caatgcgccc aattactccg aagctctggc aaatgctgcc
     3121 cgttaaagaa cgttcccgct ttctccgtca cgtgcggcat tactgggatg tgtgccgtca
     3181 ccgaatggcc actgaacctg ccgatatcct ccaccgattg atggaagatg gcgagctttc
     3241 agttgtcgct ggacggctgg tgtcattgac tgagcaggct gattcagtcg aggtaacact
     3301 tcggttgcgt cagcagcacg acctccaacg cctgaaggtc agttcggtag tgaactgtac
     3361 cggacctgaa acaaatattg cccggctggc agatccattg atgcagtctc tgtgtgtgca
     3421 aggggtgatt cagccagatg ccctcggact cggacttgaa ctctcagaca aaggggctgc
     3481 catcaaccaa aatggccagg cgtcacaggt tttgtactac gttgggccat tgctcaaagc
     3541 ccggtattgg gaaagcatcg ccgttcctga gttgcgaatt cacactgcca ccctggccga
     3601 gaccattcgg aactcatttg aagccgcacc cgccggggtc acggcgttga gcgaatagca
     3661 gtcagcggca caaatcaaag ggacaacaag gacagaaagg acgcaacaga caaaaaagaa
     3721 cccgaactct gaaccctgaa ccttaatatg tggttacaca ccagtttttt ccttcaatct
     3781 gagaaacagc actcagccac atctggtgac tggcggcgaa ggtgtggtgt gatttgcctg
     3841 cgtctggtgt tcagtctggt tgtcttgttt ggggtggagt ttccagcatc gagccagatt
     3901 tatcggttta agtcctacac gtcgagttct ggtctgccaa gcaatgtgat ctacagcatg
     3961 tttcaggaca gtcggggctt tttgtggatt ggaactgata gcggggtctg ctgctatgat
     4021 ggtgccgggt ttcaaatcta cagcaaagcc caggggctgc ccgacaccaa cgttcgggcc
     4081 atgtgtgaag acaagcaggg acagttgtgg gtgacgactg agggcggagt cagtcgcttt
     4141 accggcaatg ggtttgaaac atttaccacc gcccagggcc tttccaacaa tcgcacctat
     4201 tcggcaactg ttgggcggga tggcacatta tggtttggga cggccaatgg cctgaatcgc
     4261 tttgatggaa aacggtttac gacctatggt actcaacagg gcctgcctgc cgaacgaatt
     4321 tgggcgcttc tggaagattc caagggcacg ctctggatcg gtcagcgtgg cggtgggttg
     4381 attcggtttc aggatggcgt ctttaccacc ttcaatcaat ccaatggctt gcctgatgat
     4441 cgtgtctttg gtctggcgga agatccggct ggcggattat ggatcaccac cagcggtggc
     4501 ctgtgttttt ttaatggcag cacgtttcga atctacactc aggctgatgg tttaggcagt
     4561 aactcgactg ataaagtctt cattgaccgg tatcatcgcg tgtggtgctc gacgtttggc
     4621 ggcgggttgt gtcgctttga aaatggtcag tttgtggttt tcaatcgggc gcatggattg
     4681 ctcgataatt atttgacggc gttgcttcaa gatcgagaag gcaatatctg gtgtggcact
     4741 cgctcaagcg gcgtgtgtcg gttgagtagc gagttgtttg cttcctacac ttcagccacc
     4801 ggaattacca atggcacact gacgggtgtg gcacagactc cagatggcac gctctggttt
     4861 tcctcgcttg gcggcgggct ttcgagtctc agcaaagctg gtgagattcg tctctatggg
     4921 ttgaaggaag gattgccgga cctggattta tggacggtcc tggccgatag tcagggccga
     4981 atctggatgg gcggggcgcg tggcattgcc tgtcttcaaa atggacgcat cgccagctat
     5041 tcacgcgaac aaatcggggc ccgagatcgc atcacggcga ttatcgaaga tcgccagggc
     5101 cgcttatggt ttggctcaca cccaagtacc agcaacgggg tggtgatgtt tgatggcacg
     5161 aaatttcagc ttttcaccac tgaacaggga ttacctcaaa acccggtttc cagttttacg
     5221 ctcgatgctg atggcacgct cctggcctgt tccgaagggg gcttgagcca gtttgacggg
     5281 gtgcgctttg tcccgcatcc ggtccaggca ctgctcgaag accgtcggat tcgatgtgtc
     5341 tatgtggatg agcagaaaat tttgtggatt ggttcactca acgggctatt tcgctatgac
     5401 ggaacacgag tccagcactt tacccgccag gatggtttgg ccagcgatac agttcgcgcc
     5461 atcatcagtc tgcggggtga aatctggatt ggaacttcgg gtggaatctc ggtttatgat
     5521 ggcaaaacct ttcggaatta caccacccga gagggcctcc tcaatgatga gattttcacc
     5581 agtgcctgtc tgcgtgaacc agatggcacg ctctggtttg ggaccaatga tggtgcgctc
     5641 cgctatcgag ccatgccgga aattgctgta ccagccgtgc caaaggttat gattcgacgg
     5701 gtgctggttc gcgatgaacc ccaggctgac ctggcacagt tgcaattgcc atataaccaa
     5761 aacacggttc agttcgattt tgtcggcgtg tcgctggttg atgaagacgc cgttgtctac
     5821 cggcatcagc ttgaagggtt tgaacgaggt gactggtcag aagccactcg cgaacgctca
     5881 attcggttta ccaatttgcc ccctggtcag tatgtgtttc gcgtccaggc cggactgcac
     5941 aaaaaacaat ggggcgatac catggcactt gaactgaccg tgcagcgtcc attttggcaa
     6001 acctggtggt ttgggctggt gatcctggcc agtggttctg gaattgcctt ttccctctat
     6061 cggcgtcaga ttcgccagct tgagcgccgc gaaacccgca aggtggcgtt gctgcgtcac
     6121 attcaggaac agcggctggc cagtttacgc gaattgctcg aaagcattcg gacgatcaat
     6181 tcacaacttg atgtgaatat tgtgctgcag catctggccg aagagggggc ccgccttgtc
     6241 aacggagagc ctggtggcat cgggctggtt gaaaatggtc aggtcgtatt tcggcggttg
     6301 tggcttggtg gtcggtggga agagtatcag ttgcgatttc agcttgggga agccgtggcc
     6361 gggaaggtgg ctgaaactgg gaaggcggtt attgtcaatg atccaaccgc ttcgcctgaa
     6421 attgtctttc cagagctaat ccagatgtat tatgtcaacg gctggattga cgtgccgatt
     6481 gtatcccgca ccggaaaaat tgtcggtgtc ctggatgtgc gccgcggaaa agatcagcca
     6541 ctgtttggtg agtcagaccg gcaactgatt gaatcacttg ccaatcaggc tgcaattgcg
     6601 attgaaaatg ccgggctcta tagcgaactc gaagaaaaga acctgcaact ggaagagaaa
     6661 aatctgacga ttgccgagtc atttggtgag ttggaacggc tctataagaa agagcaggaa
     6721 atcacccaaa cgctgcacga actcaaccgc atgaagacca acttcatgat tgttacttcg
     6781 cacgaaatgc ggacaccgct gacggtgctc aagggctata tcgaagctct ggtcgaagaa
     6841 tatttaggtc cgctgacctt cacccagcgt cggtcactcg atacctgcca gcgcatggtt
     6901 gaacgcatgg tcgtgacctt tgacgatatt ctggaaatgc tcaagatcaa cgaaggccgg
     6961 attgcgctcc ggccaaacca gtttgatctg actgaaatcg tccaaaaaat tgtgggtgat
     7021 ttgcagactt ttgtggagcg acgcaagcag caggttttct ttgatgctcc tgactcagtc
     7081 gtactttccg ccgatcaggg aaaaatccag atggtgctca tcaacgtcat ccagaatgca
     7141 attaaattta cccacgatgg tggaacgatt cacattaccc ttacccagga aaatgattgg
     7201 gcgaaaatct caattgctga ttctggaatc ggcatcagtg ctgaagaggt cgggcgcatt
     7261 tttgaaaagt tctataccag ccaggatcca tcaacccaca cgtcaggaaa gtttgagttt
     7321 tcagctcgcg gcaccgggct tgggctggcg attgccaaga gttatgttga tgcccacggt
     7381 ggacgtatct gggctgaatc agaaggcacg gggaagggga caaccgtgtt tattcatctg
     7441 ccctttgcca gtaagtcgtc ccacgtcgag ttacccaaaa ccggcaagtt caatgttatc
     7501 cagaaggacg attcgtagtt ggaatgatct catcgtgtca gcaaaaggct aaagtattg
//
LOCUS       DAOQDO010000023         7528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5572_length_7528_cov_3.361970, whole genome shotgun sequence.
ACCESSION   DAOQDO010000023 DAOQDO010000000
VERSION     DAOQDO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5572_length_7528_cov_3.361970"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKZ53_00780"
     CDS             complement(<1..453)
                     /locus_tag="PKZ53_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38996.1"
                     /translation="MFDQLRLFETAWCTSGITPPYSTSMIDVENPKLNLTTKSLPAEK
                     TPATPASRVSMTGWVQYAVAWVILKSLTHLPRRAAIFFGEQVAAFIFLCIPRLQRVGM
                     RNLELAFPEKPLTERKTILKQSICSLGRQLGEFSQLPKLTAETVRSLVV"
     gene            complement(459..1445)
                     /locus_tag="PKZ53_00785"
     CDS             complement(459..1445)
                     /locus_tag="PKZ53_00785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNJ38997.1"
                     /translation="MATQKSQLLAELQNLEKQAAHLASHPTTPSHEKELDTLRERIQS
                     LQTQILGSTAATPPGLTPIEKVRLARHPERPYTLEMVQELFTDFQEMHGDRRYADDPA
                     LVCGFARFQGDPVLVVGHQKANLTGRKMEDRKYRNFGMPKPEGYRKALRAMQFAAKFG
                     RPIFSLIDTPGAYPGIDAEERGQAEAIARNLIEMARLPVPIIITVLGEGGSGGALAIG
                     VGDRVNMLENAVYSVITPEGCAAILWKDAAKADRAAASLKITAQELKAINLIDEVIPE
                     PGDGAHTDHQQAAKLLQPCLERQLSELKTLSVQDLLAQRYEKFRRMGVFTEL"
     gene            1992..2192
                     /locus_tag="PKZ53_00790"
     CDS             1992..2192
                     /locus_tag="PKZ53_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ38998.1"
                     /translation="MPRGSCPECGADVHVSEEIDKGEYTECDECGAELVVVGLDPVEL
                     DLREDYEDDEDDDYYDDDDEDE"
     gene            2262..2720
                     /locus_tag="PKZ53_00795"
     CDS             2262..2720
                     /locus_tag="PKZ53_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNJ38999.1"
                     /translation="MLYGYDMGKAVQMMREELTHHGVRELRTPAEVDQVLSPRQGTVL
                     LVVNSVCGCAAGAARPGVVQALQAATAQPDQVTTVFAGQDRDATERARTYLTGFAPSS
                     PSVWLLKDGQVVFKLERYQIEQRSAQAIAADIVKGLNTFCAPAQQAVSGD"
     gene            3035..3775
                     /gene="cmk"
                     /locus_tag="PKZ53_00800"
     CDS             3035..3775
                     /gene="cmk"
                     /locus_tag="PKZ53_00800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNJ39000.1"
                     /translation="MSTILRNHIVVAIDGPAGAGKSTVGKLLAAQLGALYIDTGAMYR
                     TIAWLGLQAGILHPGHHTTPLTPDQEQALTNLADTTEVRLTGDPFHLRVWANTEEVTT
                     AIRTPEVTRYASVGSAIPGVRMAMVAQQRQMGAVGSVVMDGRDIGTYVFPDARFKFFL
                     DASVETRSQRRFAELQAKGDPTPLDVIQAEVIERDHRDRTRAMAPLLQAADAIYIDTS
                     GLSPEKVVEEMVTVISQSTRPSPELASR"
     gene            3890..4555
                     /locus_tag="PKZ53_00805"
     CDS             3890..4555
                     /locus_tag="PKZ53_00805"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNJ39001.1"
                     /translation="MFTGIIEELGTVQRLERQTSGGQVLIGAKQVLQESRCGDSIAVN
                     GVCLTVTAFTDHWFTADVSAETLAKSNLGWLAPGSIVNLERALALGQRLGGHIVQGHI
                     DATGTFLSRKSLGNSVVMRFGFPSTIGQYIALKGSIAINGVSLTVAELSEQWFEVAVI
                     PTTLNWTNLPKLQSGDSVNLETDVLAKYLERLLERKQSKSPYDATSPASSLTLDQLRA
                     LGF"
     gene            4646..5785
                     /gene="ribB"
                     /locus_tag="PKZ53_00810"
     CDS             4646..5785
                     /gene="ribB"
                     /locus_tag="PKZ53_00810"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNJ39002.1"
                     /translation="MSPFSTIEAALEDLRDGKLIIIVDDEDRENEGDLACAAEKVTPE
                     LINFMARHGRGLICLALTEERCDELQLPLQVSDNTSRFGTAFTVTIEARQGVTTGISA
                     ADRATTILTAIDPQTTPRDLARPGHTFPLRARRGGVLVRPGQTEASVDLARLAGLKPS
                     GVICEILNDDGTMARLPQLQVFAEEHKLKIVSVADLIRYRLTNEVLVKKTAEATIETP
                     YGPFQACSFRSEVTNEVHLALVKGNIASQDSVLVRVHSQTVLGDVFEDRQDPAGGWLR
                     SALKHIAEEGCGVLLYLRQSDGGEHLENHLRAHREAQSGQPVNFTHGNSSDYGIGAQI
                     LRALGLRKIRVLTNHPMRLTAIEGFGLTFVETVPIHPSHQTPVQR"
     gene            5932..7230
                     /gene="hemL"
                     /locus_tag="PKZ53_00815"
     CDS             5932..7230
                     /gene="hemL"
                     /locus_tag="PKZ53_00815"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNJ39003.1"
                     /translation="MAQIDTSRSEKLFQRAQAVIPGGVNSPVRAFRGVGGTPLFIRSA
                     QGPYLTDVDGRRFVDYIGSWGPMILGHGHPEVLEALRKALERGTSYGAPTEIEVEMAE
                     LICSMVPSLEKVRMVSSGTEATMSAIRLARGYTGRNKLVKFIGCYHGHGDPLLVKAGS
                     GVATLGLPDSLGVPPETAQHTIAVPFNNLEALRQVFETWSNDIACVIVEPVVGNMGCV
                     APQPGYLEGMRELTQTYGAVLIFDEVMTGFRLAHGGAQERYGVKPDLTTLGKIMGGGL
                     PAAAYGGKREIMDYVAPLGPVYQAGTLSGNPLAMTAGLTTLKLLQQPGVYDNLERLSN
                     KLVQGLRHAADKAGFKTVINSVGSMFTLFFTSTPVTDWETAKTSDTQLFAKFFHAMLA
                     EGVYFPPSQFEAAFFGLAHTNEIIDSTIAASERAFGKMKN"
BASE COUNT         1827 a         1959 c         1980 g         1762 t
ORIGIN      
        1 cacaaccaga gatcgaaccg tttcagccgt gagtttcgga agctggctga actcgccaag
       61 ctgccggcca aggctgcaaa tggattgttt gagaatggtt ttgcgttcag taagaggttt
      121 ttcaggaaag gcgagttcca gattgcgcat ccccacccgc tgtaaccggg gaatacagag
      181 gaaaataaag gcggcaacct gctctccgaa gaagattgct gcccgacgag gcaggtgggt
      241 caggcttttg agaatcaccc aggcaaccgc atattgcacc catccagtca ttgaaaccct
      301 ggaggcaggc gtcgccggag ttttttcggc tggaagcgat ttggtcgtta gattgagctt
      361 ggggttttca acatcaatca tggaggtgga gtacggcggc gtgatgccgc ttgtgcacca
      421 ggcggtttca aacagccgaa gctggtcaaa caattcaatt agagttcggt aaaaacgccc
      481 atccgccgga atttttcata ccgttgggct aagagatcct gaactgaaag cgtcttgagt
      541 tcagaaagct ggcgttccag gcagggttgg agcaatttag ccgcctgttg gtgatcagta
      601 tgtgccccgt ctccaggttc gggaatcact tcatcaatca gattgatcgc tttgagttcc
      661 tgggcggtga ttttcagact ggctgcggcc cggtccgctt tggcggcatc tttccacaaa
      721 atggcggcac agccttcagg tgtaatcacc gaatacactg cgttttccag catattgacg
      781 cgatccccca ccccaatcgc cagcgcccca ccagatccac cttcacccaa aacggtgata
      841 atgatcggca ctggcaaccg ggccatctca atcagattcc gggcaatggc ttcggcctgg
      901 cctcgctctt cagcatcaat gccaggatag gcccctggag tatcaatcag gctgaaaatt
      961 ggacgaccaa atttggcagc aaattgcatg gcccggagcg ctttgcgata accctcaggt
     1021 ttgggcatgc caaagttacg atacttgcgg tcctccatct tacgcccggt caagttggct
     1081 ttctggtggc caaccaccag gacggggtca ccttgaaacc gcgcaaagcc gcagaccaga
     1141 gccgggtcat ccgcatagcg gcgatcacca tgcatttcct gaaaatcggt aaaaagttcc
     1201 tgaaccatct ccagtgtata gggccgttct gggtgacgtg ccagtcggac cttttcaatc
     1261 ggagtcaatc ctggaggagt ggccgccgta ctccccaaaa tttgtgtctg aagcgactgg
     1321 atgcgctccc gcaaagtatc aagttccttc tcgtgtgacg gagttgttgg atgagaggcc
     1381 agatgagctg cctgtttttc caaattttgc aactctgcca gcagttgcga tttctgtgta
     1441 gccatagcaa acattcccgc agacagtgat aatgaccggt atgggtgaaa tttccccgcc
     1501 cctttcgcta aaattcgctg ccaatccgtc attgacgcac cccaggtcct tcccacggaa
     1561 ccagtgtatt tttaaccacc aggccgaagg aatgtcctga aactacgtca gaagtgatga
     1621 gatggagcag cggatgtagc caccggatgt actcagaagc gaagtatctg attgaggttg
     1681 tgagtaggtg agactctacg ccgccttgtc agatgcgtca atgcttctgt tcgactgcac
     1741 ctgaccaatc aatctgatcc tccactggaa ggaatccggt tcatttggca cagacaggcc
     1801 caggacgggc gcttgggatg atcactcgtc aaccaggcaa aaagttttgg gtggttgcaa
     1861 ttcagaaggg cttggattac attttgccca ctttccaggc aattcaagcc actccagcta
     1921 tcgaaagctg aacggaactc aagttacaac cgcaacagta ttcacatttt atctagcgag
     1981 gtgtttcgca tatgccaaga ggaagttgcc ctgaatgtgg ggccgatgtt cacgtgagtg
     2041 aagagattga taagggagag tacacagagt gcgatgaatg tggcgccgaa ctcgtcgtgg
     2101 tcgggcttga cccagttgaa ctggatctgc gcgaagatta tgaagacgac gaagacgacg
     2161 actattatga tgatgacgac gaagacgaat aaggacaggt aacggattgt ccctgtttac
     2221 gttgttattt atcaggtcag gattttagaa gaaggaaccc tatgctttac ggatatgata
     2281 tgggaaaagc cgttcaaatg atgcgtgagg aattaaccca tcacggcgtt cgagaactgc
     2341 ggaccccagc cgaagttgat caggttctgt ctcctcgtca ggggacagta ctgcttgttg
     2401 tcaattcagt atgcggttgt gcggcgggtg cggctcggcc aggcgtggtt caggcactgc
     2461 aggcagccac tgcccaacca gatcaggtca caactgtttt tgcgggccag gatcgcgacg
     2521 cgactgaacg tgcccgaacc taccttaccg ggtttgcccc atcatcccca tctgtctggc
     2581 ttttgaaaga tggtcaggtg gtgtttaaac tcgaacgtta ccagattgaa caacgcagtg
     2641 cgcaggcaat tgccgccgat attgtgaaag gactcaacac cttttgcgcc ccggctcaac
     2701 aggcagtcag cggtgactaa ccagcctgaa gccctcaggc ttgaaaccat ttcggtacag
     2761 gcttgaaaat cggcgatata tgcagttgtc gcttgaccgc gaccgtttca aaccgcactc
     2821 atccgatctc tgatttatct taaaaaagtg ctctttattc gggaaggctc gctttcaacc
     2881 ggggaaagcc gaatctgaag agtgcctgaa tatcagtttt ctctgttcgc ctgtttgccg
     2941 ttacgctctg ggcagacagg cgttctttct atggcctgac tttcggtctg ggagtatcac
     3001 cctacagaca acggactggt catctgttat acacgtgtct accatcctcc gtaatcatat
     3061 tgtggtggca attgacggtc cggctggggc cggtaaaagc actgtgggca agctgctggc
     3121 agctcaacta ggcgcgctct atattgatac gggtgcaatg tatcggacga ttgcctggtt
     3181 ggggttacag gctggaatac tccacccagg gcaccacacc actccgctca ccccagatca
     3241 ggaacaggcg cttaccaacc tggccgatac cactgaggtc cgattaactg gcgacccatt
     3301 tcacctgagg gtttgggcca ataccgaaga ggttacaacg gccattcgaa cgccggaagt
     3361 gacccggtat gcttcagtcg gttcggccat tcctggagtt cgaatggcca tggtggctca
     3421 acaacggcaa atgggtgccg tgggaagtgt cgtcatggat ggacgcgaca ttggaaccta
     3481 tgtctttcca gatgccagat tcaaattttt ccttgatgcc tcggtcgaga ctcggagcca
     3541 gcgccgcttt gctgaattgc aggcgaaagg ggaccctacc ccactggatg tcatccaggc
     3601 cgaagtcatc gaacgggatc atcgggatcg gacccgagcc atggcgccgc tgcttcaggc
     3661 cgctgacgcc atctatattg acacttctgg cctgtcaccg gaaaaagttg tggaagaaat
     3721 ggtcacagtt atttcacaat caacccgacc ttcccctgaa ctggcctcac ggtagaatca
     3781 cccctggcat ctgacctgcc gcacgcccaa tgaaagggtg aatctctgga cacacatcac
     3841 accagagaca ccacgttcat aaccttcccc tgacccgtgc tcacctttta tgttcactgg
     3901 aatcattgaa gaattgggaa cagttcaacg attggaacgc cagacatcag gcggccaggt
     3961 tttgattggt gccaaacagg tgctgcaaga aagccgctgt ggcgacagca tcgctgtcaa
     4021 cggggtgtgc ttaacagtga cggcttttac agaccactgg tttaccgctg acgtatcggc
     4081 tgaaaccctg gccaaatcca atctaggttg gctggcacca gggagcatcg tgaatctgga
     4141 gcgggcactg gctcttggtc aacgacttgg aggccacatc gttcagggac acattgatgc
     4201 caccggcaca ttcctgagtc gaaaatctct gggcaattcc gttgtgatgc ggtttggctt
     4261 cccatcaact attgggcaat acatcgctct caaaggcagt attgccatca atggtgtcag
     4321 tttgacagtc gctgaattga gcgaacagtg gtttgaagtc gcggtgattc cgaccacact
     4381 caactggacc aatttaccga aactgcaaag cggggattcc gtcaatcttg agacagatgt
     4441 gctggcaaag tacctggagc ggcttctgga gcgaaaacaa tcaaaaagcc cctatgacgc
     4501 cacctcacca gcatcgtcgt tgacgctcga tcagttgcga gcacttggat tctaatccca
     4561 cccgggttcc gggttttcga acttttgctc ttttcgtcct gtttgtcctt tttgtctttt
     4621 aaaccctgaa ccctaaaagg gaactatgtc acctttttcc acgattgaag cagcactcga
     4681 agaccttcgg gatggcaagc tgattattat tgttgatgat gaggatcgcg aaaatgaagg
     4741 cgatctggcc tgtgctgccg aaaaagtcac gccagagttg attaacttta tggcgcgaca
     4801 cggacgcggc ctgatttgtc tggccctgac cgaagagcgg tgtgatgaac ttcaattgcc
     4861 gcttcaggtc agcgacaaca cctcccggtt tggcacggcg tttacagtca ctatcgaagc
     4921 cagacaggga gtcaccaccg gcatttcagc cgctgatcga gccacaacca ttctgaccgc
     4981 gattgatccg cagaccacac ccagggatct ggctcgacca ggtcatacct ttcccctgcg
     5041 tgcccggcga ggtggcgtgc tggtcagacc tggacaaacc gaagccagtg ttgacctggc
     5101 tcgattagca ggattaaaac catctggggt catctgtgaa attctcaatg acgacggcac
     5161 catggcacgg ctaccgcaac ttcaggtatt tgccgaagaa cataaactga aaatagtttc
     5221 cgtcgcggac ttaatccgct atcgtctaac gaacgaagtt ttggtaaaaa agacagccga
     5281 agcaacgatt gaaaccccct acggtccctt tcaagcctgt tcgttccgca gtgaagtcac
     5341 caatgaagtc catctggcac tggtgaaagg caatattgcc tcccaggatt cagttctggt
     5401 tcgagttcac tcacaaacag ttttgggcga tgtctttgaa gacaggcagg atccagccgg
     5461 tggctggctc cgatcagcac ttaaacatat cgcagaagaa gggtgcgggg tcttattgta
     5521 cttgcgccaa agtgatggtg gcgaacatct tgaaaaccac ttgcgcgccc accgtgaagc
     5581 ccaatctgga cagccagtaa acttcaccca cggcaacagc agtgattatg gaattggtgc
     5641 ccagattttg cgcgccctgg gtcttcgtaa aattcgggtg ctcacaaacc atccaatgcg
     5701 gttgacagct attgaggggt ttggattaac atttgttgaa acggtaccaa ttcatccctc
     5761 tcaccaaaca ccagttcaac ggtaaagccc tttgctgacg gtgacttaca ggtttagaat
     5821 ccctaaacca gcctgtcttg acaaaatact gcaccgggtg aagccaacca aaatcttgcc
     5881 ccgatacgac aaaaatatac aatttgggat ccagcaacta aggagtttta catggcacag
     5941 atcgacacaa gtcgttccga aaagcttttt cagcgtgcac aggcggtcat tccgggcggc
     6001 gttaatagtc cagtacgggc attcagaggc gtgggcggaa cgccattgtt tattcgctcg
     6061 gctcaggggc catatttgac tgatgtcgat ggccggcgat ttgtggacta tattggctcc
     6121 tggggaccga tgatcctggg ccatggtcat cctgaagtcc tcgaagcatt gcgaaaagca
     6181 ctggaacgcg gcacatcata cggggcgcca actgaaattg aagttgaaat ggccgagttg
     6241 atttgttcga tggtgccatc gcttgaaaaa gttcgcatgg tcagttccgg caccgaagcc
     6301 accatgagcg ccattcgttt agctcgtggg tataccgggc ggaataaact ggtcaaattt
     6361 attgggtgct atcacggcca tggcgatccg ctcctggtca aagctggctc tggagtggcc
     6421 accctggggt tgcctgatag cctgggggtg ccaccagaaa cagcacaaca tacgattgca
     6481 gttccattta acaacctgga agcactccgt caggtatttg aaacctggtc caacgatatt
     6541 gcctgtgtca ttgtggagcc ggttgtgggc aatatgggct gtgtcgcccc acaacctggc
     6601 tatctggaag gcatgcgaga gttgacccaa acctatggtg ccgtgctgat ttttgatgaa
     6661 gtgatgactg ggttccggct ggcgcatggt ggtgcgcagg aacgatatgg agtcaagcct
     6721 gatctgacca cattgggcaa aatcatgggc ggcggactgc cggctgcagc ctatggcggg
     6781 aaacgagaaa ttatggatta tgtcgcccca ctgggtccgg tgtatcaggc gggaaccctc
     6841 tcaggcaacc cactggccat gacggctgga ctcaccacat tgaaactact gcaacaacca
     6901 ggggtttatg acaacctgga acggctttca aacaaactgg tccagggcct gcgccatgca
     6961 gcggataaag ccgggtttaa aacagtgatt aactcggttg gctctatgtt tacattgttt
     7021 ttcacctcaa ccccggtcac agattgggaa acagccaaga ccagtgatac ccagcttttt
     7081 gccaagttct ttcatgcgat gctggctgaa ggcgtctatt tcccgccctc gcagtttgaa
     7141 gcggcttttt ttggtctggc gcataccaat gaaatcattg actcaaccat tgcagcttct
     7201 gaacgcgcat ttgggaagat gaaaaactag agagaaccag ggctgaagaa tctgggctga
     7261 agaaccaggg ctgaagaacc tgggctgagg gcttggggct gaagactcgc aagctcaggg
     7321 caatcgaagg gatgaggaat gaaggatggg ggatgaaata aaaccaactt cttcagccca
     7381 actttttcag cccgagatgt cttcaaccca acttcttcag cccgacttct tcaacccaac
     7441 ttcttcagcc cgacttcttc agccccacct tcaccaactc cacgcctatt cccgatgaaa
     7501 cccagccacc actggcacat ggtcagaa
//
LOCUS       DAOQDO010000024         7496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5609_length_7496_cov_3.532993, whole genome shotgun sequence.
ACCESSION   DAOQDO010000024 DAOQDO010000000
VERSION     DAOQDO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5609_length_7496_cov_3.532993"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..616
                     /locus_tag="PKZ53_00820"
     CDS             140..616
                     /locus_tag="PKZ53_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39004.1"
                     /translation="MKNEEPKPDPLKQKAQLRKYHLALSRTLESITGKTVPDEAAFRE
                     LETYSQGWHKIGLLAIAIWEQEAFHPQAFEAHPGWEAAANTTFEFLVEHCSNCGVKQV
                     DLKTVIFCPPCRRTVSEEQFQSVMDGPVAQEVLAFAEELLALESESDDASGSSFIS"
     gene            complement(643..1815)
                     /gene="bioF"
                     /locus_tag="PKZ53_00825"
     CDS             complement(643..1815)
                     /gene="bioF"
                     /locus_tag="PKZ53_00825"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNJ39005.1"
                     /translation="MRNIQSLHDELSSIRQQGRYRSLRTVSSPPEAEIIIDGTRLLNF
                     SSNNYLGLATHPHLKIAAEAATQRWGTGSTASRSICGNLELHQQLEASLARFKGSQSS
                     PVRALLFNSGYHANTGIIPALMQEGDVIFSDALNHASIIDGSRLSRARTVVYAHRDMT
                     DLAVKLENHTSARRKLIVTDSVFSMDGDTAPLTEIVELASRHNAWVMVDEAHGTGVLG
                     EQGRGLVNQLGLTDRVELQMGTLGKALGSFGAYVVAPEPFIELLINRARSFIFTTGLP
                     PAPVAAAIEALNLIQAEPDHLCRLHQAMHYFGTGLCELGLLQTVPEAPIYPVVLGDET
                     QTMQAMEFLKTRGIFAQGIRPPTVPAGTCRLRLTVMATHTRAHLDQALEALQVLGK"
     gene            complement(1855..2028)
                     /locus_tag="PKZ53_00830"
     CDS             complement(1855..2028)
                     /locus_tag="PKZ53_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ39006.1"
                     /translation="MNYTIETERETDGRWIAEVIELPGVLAYGETQDQARIKVQALAF
                     RVIAEHLENGESI"
     gene            complement(2187..4487)
                     /gene="purL"
                     /locus_tag="PKZ53_00835"
     CDS             complement(2187..4487)
                     /gene="purL"
                     /locus_tag="PKZ53_00835"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNJ39007.1"
                     /translation="MSASSPLTAEVIAAHGITPSEFDHIVALLGRTPNLVELGIFSVM
                     WSEHCSYKSSRIYLKNLPTTGERVVQGPGENAGVINIGEGWCIAFKIESHNHPSFIEP
                     FQGAATGVGGILRDIFTMGARPVALLNSLRFGRLDDPQYGPRNRAIFNGVVRGIGHYG
                     NAFGCPTIGGEVYFADCYSPNPLVNAFALGLVREDQIFFGRAKGISNSVLYVGAKTGR
                     DGIHGATMASAEFDDEALSKRPTVQVGDPFCEKLLLEACLEAMRAGCVVGIQDMGAAG
                     LTSSSCEMGARAGNGIDIDVALVPQRETGMTPYEILLSESQERMLLVAAPGREREVQE
                     IFARWELSAVIIGHVIQEPVMRVRHFGEIVADIPNTYLTDEAPRYQRPCAAPAGMELP
                     LVERLRNEQAVLDEVLPPAARNLSPNAVASTLRTMLGSLNLSSREWVYQQYDHMVRTN
                     TVILPGADASVVRVKETRKAIAISLDGNGRYCAIDPHRGAELLVAESCRNLVTAGAKP
                     IGLTNCLNFASPERPEVMWAFREAVAGMGVAARVFETPVVSGNVSLYNETEGRGIYPT
                     PVIGSVGLIEDIRYLISNTTVQSGDVVLLLGASFEELGATEFLERQTGQVVGPLPQLE
                     YDREQKLHSVVLESAVKGLHSGAHDLSDGGLLVALAELSFSLHGRSALGMALEMKREG
                     ALADLSIAGLLFGETPSRMLVTVQAAQLDKFLALAAEHSLPCTQLGVLGGTHLKVTID
                     GTPYLDEPVAELEHIWRTSLGTAVGI"
     gene            4676..5152
                     /locus_tag="PKZ53_00840"
     CDS             4676..5152
                     /locus_tag="PKZ53_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39008.1"
                     /translation="MPTSRQHNWFDLVLSLGSKPAACLLLLVAIQWGGLQLHIFTHST
                     GTHRGYSVEFCSVSELSSRAGTSSHRPCETLAPQTSSDSWLTEPESDQPAPSLTSQCP
                     LCILALELHNLSPPDTCTPCLEILADVQHRLVALSVYSHLLASLQSSRAPPTFTVG"
     gene            5382..>7496
                     /locus_tag="PKZ53_00845"
     CDS             5382..>7496
                     /locus_tag="PKZ53_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39009.1"
                     /translation="MVSMESFFRSLAGCALSVLLILTLGSGVVSANSGSSTSGVIRGK
                     VLDATGAPIAGATITLSNPVSGYTKQTQTDSNGSYTFFNVPFNPYTIKAEAASFSPTA
                     ESIEVRSPMAMEFNLSLAVGATSDVITVSADTQSILETESATTHVDIDKSLIQRFPAS
                     TATRGMESIILSTPGFMADENGRFHFRGSHGQVTYVVDGVPVSDQLNLTFSNSLDPNN
                     VESMEVITGNMLPEFGGRNAAVINVTSRSALGSARKFFGNLTLGGARFSTGEINAQFG
                     GSISDKFGYFVSLGATRSNRFSDPVNFENLNNTGNTQRSSVRFDYAATPNDFLRLSLG
                     AGRTERAIPNLFSQHAAGQNQNTLNKDFSINFTWQHTFNNKVSTDITPYFRTSSAQLL
                     ELTPFSTPLSAQQDRHLSNYGLNASVSYDEAMGEFHNHFKAGLNFFAFPVSENFNFAI
                     TDPNFNAPPETAGRRDEEEESTFNPNLAPFDLTRGGTRFQFSDRDKGKQYSFFVQDSV
                     TYKGLTLNGGVRYDNYRFKAIEDSWQPRVGIAYHINQTGTVLRAAYNRIFMTPFNENL
                     LLASSDQAAALAPPFVVEAQGTARSVVRSERQHSYEVGIQQRLKNLLRFDFSYWTKDG
                     RQASDNLQFLNTGVVFPVAFASSKLRGADFRVDMPNYRGFNAYWSIGTARAVYTPPFT
                     GGLFLDPEVAELVNSGPFRIDHD"
BASE COUNT         1770 a         1985 c         1983 g         1758 t
ORIGIN      
        1 cgcagcaaaa gatctttgag tggttgattc ccaaataatt gatcaaatga cataagactc
       61 gaatgaagaa ttgagaatga agaattgaga atgaagaatt aagaatgaag aattgagaat
      121 gaagaattga gaatgaagaa tgaaaaatga agaacccaaa ccagatccgc tcaagcaaaa
      181 ggcccagttg cgaaaatatc atctggcgct gagccgaacc ctcgaatcaa ttaccggcaa
      241 aactgtgccc gatgaagccg cttttcgaga attggaaact tatagccagg gatggcacaa
      301 aatcggattg ctggcgattg cgatttggga gcaggaagcc ttccacccac aggcctttga
      361 agcccaccca ggttgggaag ccgccgcgaa cacaacgttt gaatttctgg tcgagcactg
      421 ctccaactgt ggtgtcaagc aggtagattt gaaaaccgtg attttttgtc cgccttgccg
      481 ccgaacggtg agtgaagagc agtttcaatc tgtgatggac ggacccgttg cccaggaggt
      541 tctggctttt gccgaggaat tactggcgct ggaaagtgag tctgatgacg cgtcagggtc
      601 gagttttatt tcctgaaaac gaaggaatgg cgaatttcct gtttatttcc ccaacacctg
      661 gagtgcttca agcgcctgat caagatgtgc tcgggtgtgg gtggccatca cggtcaggcg
      721 gaggcggcag gtcccagccg gaacggttgg cgggcggatg ccctgggcaa aaatccctcg
      781 ggttttgaga aactccatgg cctgcattgt ttgtgtttca tccccaagca caaccggata
      841 aattggtgct tccggcacgg tttgcagcaa tcccaattca cacagtccgg ttccaaagta
      901 gtgcattgcc tgatgcagac ggcagaggtg gtcgggttcg gcctgaatta aattcagggc
      961 ttcaatcgca gcagcgacag gtgctggtgg caggccagtg gtaaaaataa aagatcgtgc
     1021 ccgattgatg agcagttcaa taaatggttc tggtgcaacc acataggcgc caaaactccc
     1081 cagtgccttt cccagggttc ccatttgaag ttcaactcga tccgtcaggc caagctggtt
     1141 gaccagcccg cgcccctgtt cgcctaaaac cccggttccg tgagcttcat caaccatgac
     1201 ccaggcgttg tggcgcgagg ccagttccac aatctcagtc agtggggccg tgtcaccatc
     1261 catggaaaag acggaatctg tcacaatcag cttgcgtctg gctgaggtgt gattttccaa
     1321 tttgacagcc agatccgtca tatctcgatg ggcatacaca acggttcgag cacgacttaa
     1381 ccgactccca tcaataatgc tggcgtgatt gagcgcatcg gaaaagatga catcgccttc
     1441 ctgcatcaag gcgggaatga tcccggtgtt ggcgtggtat ccactgttaa agagcagggc
     1501 ccgaaccggt gatgactgac ttcctttaaa tctggccagg ctggcttcaa gttgttgatg
     1561 gagttcaaga ttaccgcaaa ttgatcggct ggcggtgctg cctgtacccc agcgttgggt
     1621 ggcggcttca gcggcaattt tcaagtgggg atgagtggcg agtccgaggt aattattgct
     1681 tgaaaaattc agcaggcgtg tgccgtcaat gatgatttca gcttcggggg ggctactgac
     1741 cgtgcgcagt gaccgatacc gcccttgctg gcggatactg gaaagttcgt cgtggaggga
     1801 ctggatattg cgcattttgg ggttgagggt tccttaaaat acacacttac aaagtcagat
     1861 gctctctcca ttttcaaggt gttcggcaat gactcgaaat gcaagtgcct gaactttgat
     1921 ccgtgcctga tcttgtgttt caccataggc aaggactcct ggtagttcaa tcacttcggc
     1981 aatccaacgt ccgtccgttt ctcgctcagt ttcaattgtg taattgatca acatggtcag
     2041 gctcctgctt caccatccgt tgtcagcagg ccataaatga cctgcctcaa ttcaaccacc
     2101 ggtgcggctg cgaaaaccaa aaacaacctg tattcgaaaa ctctgaactc tgaactctga
     2161 accctgaacc ctgaaccctg atttccctat attccaaccg ctgtcccaag tgacgttcgc
     2221 cagatgtgct caagctcggc cacgggctca tccaggtacg gggtgccatc aattgtgacc
     2281 ttcagatggg ttccaccaag gaccccaagc tgggtgcagg gcagggaatg ttcagccgcc
     2341 agggccagaa atttgtccaa ctgcgctgcc tgaaccgtga ccagcatgcg ggatggcgtt
     2401 tcgccaaaca acagcccggc aatcgaaaga tcggccagcg cgccttcgcg tttcatctca
     2461 agtgccatcc caagggctga gcggccatgc agtgaaaaac tcagttcggc cagtgccacg
     2521 agcaatccgc catctgagag gtcatgagca ccggagtgca aacctttcac cgctgattca
     2581 agcactacgg aatgcaattt ttgttcacgg tcatactcaa gctggggcaa aggcccgacc
     2641 acctgaccgg tctggcgttc cagaaattcg gtggcaccca gttcttcaaa tgaagctccg
     2701 agcagcagca ccacatctcc agattgcacc gttgtgtttg agatgagata gcgaatatct
     2761 tcaatcaggc caacacttcc aatcacggga gttggataga ttccccggcc ttcggtttcg
     2821 ttatagaggc tcacattgcc actcacaacc ggagtttcaa agacgcgggc tgcaacgccc
     2881 atcccggcga cggcttcgcg aaaggcccac atcacttcag ggcgttcggg ggaggcaaaa
     2941 ttgaggcagt tggtgagtcc gattggtttg gccccagccg tgacgaggtt ccggcagctt
     3001 tccgccacca ggagttcggc gccgcgatgg ggatcaatcg cacaataccg gccattgccg
     3061 tcgagcgaaa tggcaatggc tttccgggtt tccttcaccc gcaccactga cgcatccgca
     3121 ccagggagaa tcacggtgtt ggtgcgaacc atatggtcat actgctgata aacccattca
     3181 cgggaggaca ggttcaagga accaagcatc gttcgcaggg tgctggccac ggcattgggc
     3241 gacaggttgc gggcggctgg cggtaaaact tcatcgagta cagcctgttc attgcgaagt
     3301 cgttcaacca gtggaagttc cattccggct ggcgcggcac atggacgctg atagcgcggg
     3361 gcttcgtctg tgaggtaggt atttggaatg tcagccacga tttcgccaaa gtggcgaacg
     3421 cgcatcactg gttcctgaat cacgtggccg atgatgacgg ctgagagttc ccagcgggca
     3481 aaaatttcct ggacttcccg ttcccgacct ggtgccgcca ccagcagcat gcgttcctgt
     3541 gattccgaga gcaggatttc atacggcgtc atcccggttt cacgctgtgg cacgagcgcc
     3601 acgtcaatat caattccatt gccagcacgg gcgcccattt cacagcttga cgacgtcaga
     3661 cctgccgcgc ccatatcctg gatgcccacc acacagcccg cccgcatggc ttcaagacag
     3721 gcttcgagca acaatttttc gcagaacgga tcacccacct ggacggtcgg acgcttggaa
     3781 agagcttcgt catcaaattc agccgaggcc atagtggcgc cgtgaatgcc atcacgaccg
     3841 gttttcgcac cgacatagag gaccgaattg ctgatgcctt tggcacgacc gaaaaagatc
     3901 tgatcttcgc gaaccagacc cagggcaaac gcattgacga gtggattcgg ggaatagcag
     3961 tcagcaaagt agacttcgcc gccaatcgtc gggcagccaa aagcattgcc atagtggcca
     4021 ataccgcgca cgacgccgtt gaagatggct cggttccgtg ggccatattg cgggtcatcg
     4081 agccgaccaa accggagtga attgagcagg gccaccgggc gagcccccat ggtgaaaata
     4141 tcgcgcagga tgcctcccac gccggtggcc gccccttgaa acggctcgat aaatgatggg
     4201 tgattgtggg attcgatttt gaacgcaata caccagcctt caccgatatt gatgaccccg
     4261 gcgttttcgc ctggtccctg aacgacacgt tcgccagtgg tcggcaggtt cttcaagtag
     4321 atgcgagatg acttgtagga acaatgctct gaccacataa cggaaaaaat gcccagctca
     4381 accagattgg gagtgcggcc aagcagggca acgatatggt caaattcaga tggggtgata
     4441 ccgtgagcgg cgatgacttc tgccgtgaga ggagatgagg ctgacatagg tgttgaatgc
     4501 tccgcaaagt gagggtccga aaaacctgcc agtgtagggg aagatcgtct ggttgacaat
     4561 gcactcaggc acgttgacat tttttcgatt gaaatttaga ttgtcaggtc acgtctgagg
     4621 cgcacaccgt gcctgtaagt tcaaccctga tttttgccgt tctcatttcg aatcaatgcc
     4681 aacttcacgt caacacaact ggtttgacct ggttctctcc cttgggagca agccagcggc
     4741 ctgtctgttg cttttggttg ctattcagtg gggcggatta caactgcaca tttttaccca
     4801 ctcaacgggt acccaccgtg ggtatagtgt cgaattttgt tcagtcagtg aactttcatc
     4861 cagggcaggt acttcatcac accgaccgtg tgagaccctg gcccctcaga ccagttctga
     4921 ttcctggttg acggaaccag aatctgatca accagcccca tcgctgacaa gtcagtgccc
     4981 gctgtgtatt ctagctctgg aattgcacaa tctgagtcca ccagacacct gtaccccctg
     5041 tctggagatt ttggctgatg ttcagcatcg tctggtggca ctgtcggttt attctcacct
     5101 cttagcctca ctccaaagct cacgtgcccc gcccacgttc actgtgggat aagggctctt
     5161 tcttgactta ccctgaagaa ataacctgct tgaacggaca gaattcaagc cgctgcctgt
     5221 tgtctatctg acggcaagcg acggcgtgaa cgctgtgcct gactcattcc gcatggattt
     5281 ttcaggtgct caaaaagaga cgcctgtctc ctcctcgcca gttacctctt tagttcatca
     5341 caataacttt ccttttttct gtaacttgag gagtcatacc catggtttcg atggaatcat
     5401 tttttcgatc actggctggt tgtgcgttaa gtgtgcttct gattctgacg cttggttcgg
     5461 gtgtcgtcag cgccaattct ggatcatcaa cgtcgggtgt cattcgtgga aaagtcctgg
     5521 atgccactgg tgccccgatt gcgggtgcca caatcacgct cagtaacccg gtcagcggct
     5581 ataccaaaca aacccaaacg gatagcaatg gcagttatac gtttttcaac gtgccgttta
     5641 atccatacac gatcaaggcg gaggccgcga gtttttcccc aacggcggaa agtattgaag
     5701 ttcggtctcc aatggcgatg gaatttaacc tgtcgctggc cgttggtgcc acttcagatg
     5761 tgataacagt cagtgctgac acacaatcaa tcctggagac tgaatcggca accactcatg
     5821 ttgacattga taagtcattg attcagcggt ttccggcttc aaccgccacg cgtggaatgg
     5881 aaagcatcat tttgtctaca ccgggcttta tggcggatga aaatggacgc tttcacttcc
     5941 gaggctcgca tggtcaggtg acctatgtcg tggatggcgt gccggtcagt gaccaactca
     6001 acctgacgtt ttcaaacagc ctggatccca acaacgtcga atcaatggaa gtgatcaccg
     6061 gcaacatgct gcctgagttt ggcgggcgaa atgccgccgt gatcaacgtc accagccggt
     6121 cagcacttgg atcagcgcga aaattctttg gaaacctgac gcttggcggt gcccgattta
     6181 gcaccggcga aatcaacgcc cagtttggag gctcgatcag cgataaattt ggctattttg
     6241 tgagcctggg tgccactcgc agcaaccggt tttctgatcc agtgaacttt gaaaacctca
     6301 acaacaccgg caacacccag cggtcatcgg tcaggtttga ctacgcggcc actccaaacg
     6361 atttcttgcg gttgtcgctg ggggcgggtc gaaccgagcg ggcgattccg aatttgtttt
     6421 cacaacacgc cgccgggcaa aatcagaaca cactgaacaa agatttctcg atcaacttca
     6481 cctggcagca cacgtttaac aacaaagtct caaccgacat taccccatat tttcgcacca
     6541 gctcggctca actccttgag ttgacaccgt ttagcactcc actgagcgcc cagcaggatc
     6601 gtcatttgag caactatggt ctcaatgcca gtgtgagcta tgacgaagcg atgggtgaat
     6661 tccataatca cttcaaagcc gggctgaact tctttgcttt ccccgtcagt gaaaatttca
     6721 actttgcgat caccgatcca aactttaatg caccacctga aactgctggt cggcgggatg
     6781 aggaggaaga aagcaccttc aatcccaatt tggcgccctt tgacctgacc cgtggcggca
     6841 cccggtttca atttagtgac cgcgacaagg ggaagcagta ttcgttcttt gttcaggatt
     6901 cggtgacgta taaaggattg acgctcaacg gcggtgttcg gtacgacaat tatcggttta
     6961 aagcgattga agatagctgg cagccgcgtg taggaattgc ctatcacatc aaccagaccg
     7021 gaacggtgtt gcgggcggcg tacaaccgaa tttttatgac gccgtttaat gaaaacctgc
     7081 tgctggcgag ttccgatcag gctgccgcgt tggcaccgcc atttgtggtt gaagcccagg
     7141 gtaccgcccg gtcagtcgtt cgctcggaac gacagcattc atatgaagtt ggcattcagc
     7201 agcgtctgaa aaacctgctg cggtttgatt tttcctactg gaccaaagat gggagacagg
     7261 cgtcagacaa cctgcagttt ttaaacaccg gtgtggtgtt tccagttgcc tttgcttcaa
     7321 gcaagctccg tggtgccgac ttccgggttg atatgccaaa ctatcgcggg tttaacgcct
     7381 attggagcat tggtacggcc cgggctgtct acacaccgcc atttaccggc gggttgtttc
     7441 tggaccccga agttgccgaa ctggtcaact ccggtccgtt tcggattgac cacgat
//
LOCUS       DAOQDO010000025         7298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5851_length_7298_cov_3.616043, whole genome shotgun sequence.
ACCESSION   DAOQDO010000025 DAOQDO010000000
VERSION     DAOQDO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5851_length_7298_cov_3.616043"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PKZ53_00850"
     CDS             <1..1146
                     /locus_tag="PKZ53_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39010.1"
                     /translation="VIFGVATVIVVVSLIEGFNTYVDEKISDMGPNAIVIRKFSIDDF
                     NSLDAFEKAQKRNKDIKLEDLEAIKTQSRYAVLVGAQTGGGAEVKRGDEILYEVSLTG
                     RTDTMGDIQRMEATQGSYFTFQDVEHKRQVCFVGSEIAERLFKTANPIGGEIKIDGRV
                     FSVVGVSKSLGSAFGASRDKFIDIPLTTFQKIYGTRRSLAVYVQVANEQVVPDAVDEI
                     RVIMRNRRHLSFGDLDNFGITTVEMISNLRQRIFGTIQMTAIGVTSIALVVGGIVIMN
                     IMLVSVTERTREIGIRKSLGARRTDIIRQFLAESFVLALVGGAIGVTIAYMIALSLAT
                     FTPIPARLPFGAVFLALLVSGGVGIASGVYPAWRAASLDPIRALRAD"
     gene            1176..2438
                     /locus_tag="PKZ53_00855"
     CDS             1176..2438
                     /locus_tag="PKZ53_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39011.1"
                     /translation="MNQLMNNLLMGFDTLKAHKLRTFLTILGVIVGTSTVIVIAAFVS
                     GINVQVTKEIEAWGTNTIYVYKFNPGFNFRPTLEERTRKPITKKEAVAIREGCPSVRE
                     VALFLSPIDFSNEGGRLERRIPIRYQDHEMRNSTLQGTWPAYERMGVVNLSEGRFFTE
                     EENTRCDRVCVIGLDVAETLFPTTAALNREVLIDNYPFKVIGVLAKRDHFLVGEDDAN
                     NENKAVYIPYETCHKMYPHLDDHFVMGMAQPGELDAAVNEMTNVLRRVRKVTYNDPDN
                     FGISTSDNIVEQFGQITGGIFLLMVAISSVGLLIGGIGVMNIMLVSVTERTKEIGIRK
                     AIGARQRDIIQQFLIEAVTLTGIGGLLGIGIGWLLAIGIRYILPSYVPPLAPIIGIVV
                     SVAIGVIFGLWPAIKAARLDPIEALRYE"
     gene            2749..3804
                     /locus_tag="PKZ53_00860"
     CDS             2749..3804
                     /locus_tag="PKZ53_00860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ39012.1"
                     /translation="MAINLDTLRRILLVRLRSIGDTVLMTPVLEVLKQWSPEISISVV
                     SEPLSAPVLEAHPLVDQLFTVPRTRNKWRDLALRGRLIRDLRRERFDLAVNLHGGTTA
                     AWICWLSGTPHRIGFALPNTKFLLTTLIPPPQEVWQKKTIHCVEQQLAWVKYLGIPIH
                     QPPVLSLNCDAQCLKTVAAFLTDRGIASHYAVFHPAAAFTTKQWAPERFAKVIEYVKG
                     LGLKPIICVAKAEAGVAQAIQRSLVYQDETLVIDHFSLSETMALIAGASLFVGNDSGP
                     AHIAAALRRPMVVVFGSSNEDVWHPWTSAPHQIVRHHIPCVPCAGYTCHAFPEPECIK
                     GVEVKAVINAIDSVLGR"
     gene            complement(3858..4934)
                     /locus_tag="PKZ53_00865"
     CDS             complement(3858..4934)
                     /locus_tag="PKZ53_00865"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNJ39013.1"
                     /translation="MWWESIRNFFVSGVAIDLGTANTLIYSRGHGIILNEPSAIAINR
                     DSGNVVAVGRRAIAMLGREPYDTVVHRPMKDGTIADYDLAEKMLRNFFHAACKRESRR
                     YCKLVVSIPSSATQTERRALRNAAYSIGANKVFLIDEGLAAAVGSEVMFRSPQAHLLV
                     DIGGGTTNITIVSTTGIIASKSLEIAGNRMTQDIVEYMHRKHKLVIDEQSAEQIKINL
                     GSAIAQENEASMLVTGKNLTNYKVRELMLTQSEVVEALDHTLDTIIEGIRQVIELSKP
                     AVAVDIHRTGVTLAGGGSLLRDFYRRVHLELGLPAMRASNPMEAVVIGAGQLLDRPEL
                     LKRFSGADMVRYQEWVDEDMPVPS"
     gene            complement(5473..6147)
                     /locus_tag="PKZ53_00870"
     CDS             complement(5473..6147)
                     /locus_tag="PKZ53_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNJ39014.1"
                     /translation="MTGSPIFAQEAPPPKKKPAPSATSTAPGQSTSTSPKPAPTLVDT
                     LQSYLKRMNVKSEKRQGNNTTLVTHYQSGKETVDLVLVNMPSKKMLGFYAYKFGNTKK
                     VANPSPLFKTLLSLNDQLAVGAFFIDEEDDIGFKFSVRTDPQVGFDEFQSVYLGLVSA
                     VKQARPQILKHFEEQPETTQPETDTTKAPPSQESQSKLAVPKPGLRLDSSFPVTRSRR
                     VRGLGI"
     gene            6454..7239
                     /locus_tag="PKZ53_00875"
     CDS             6454..7239
                     /locus_tag="PKZ53_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HNJ39015.1"
                     /translation="MSEQKPLVSPPVKTLETELAEFARDQFRRFAPRKVLPTGFQVQF
                     YPYAGFNNTIRLRQGILLIRLSDLLKGASPVVRQAVIGILIAKILKKPVPAEYEEAFR
                     VFTQLPSVKTAIERARRVRGFKHVSGEKGHVYDLGKLFQELNHQYFSDEVSQPTLTWS
                     RTQTRRILGHYDRAHHTIVISRTLDDVRVPNYVVAYVLYHEMLHIKHKVRSVNGRNCI
                     HSSEFQREERVFAHYTEAQQWLAELARRPKPRPVRRRSPAKPK"
BASE COUNT         1882 a         1627 c         1818 g         1971 t
ORIGIN      
        1 gttattttcg gagttgccac agtcattgtt gttgtttcac tgattgaagg gtttaacacc
       61 tatgttgatg aaaaaatttc ggacatggga cccaatgcaa ttgtgattcg aaaattcagc
      121 attgacgatt tcaacagcct tgatgcgttt gaaaaggcgc aaaaacggaa taaagatata
      181 aaattggagg atctggaagc gatcaagacc cagtcccgat atgccgtatt agttggtgct
      241 caaactgggg gaggcgctga ggtcaaacgt ggcgatgaaa tcctgtatga agtcagcctc
      301 acaggccgaa ctgatacgat gggagacatc cagcggatgg aggctacgca ggggagttac
      361 tttacctttc aggatgtaga gcataagcga caggtctgtt ttgttggttc ggaaattgcc
      421 gaacggttat tcaaaacagc aaatccaatc ggaggcgaga taaaaattga tggccgggtt
      481 ttttcggtcg ttggggtttc aaaatccctt gggtctgcgt ttggtgcttc gcgtgacaaa
      541 tttattgata tcccactgac aacctttcag aaaatctatg gtacccggcg ttcgcttgct
      601 gtctatgttc aggttgcgaa tgaacaggtt gttccggatg ccgttgatga aatccgcgtc
      661 attatgcgga accgtcggca cttatcattt ggagatctgg ataattttgg aatcactacg
      721 gttgaaatga tttcaaatct gcgccagcgg atttttggga ctatccaaat gactgccatc
      781 ggcgtcactt cgattgcttt agtcgttggt ggcattgtga ttatgaatat tatgctggtc
      841 tcggtaactg aacgaacccg tgaaattggt attcggaaat cgttaggtgc ccgtcggact
      901 gatatcattc gtcaatttct ggctgaatca tttgtcctgg ccctggttgg gggagccatt
      961 ggggttacga ttgcctatat gattgcttta tctctggcaa ctttcacgcc aattccagcc
     1021 aggcttccct ttggggctgt ctttctggca ttactggtct cgggaggagt tggtattgct
     1081 tcgggtgtgt atccagcctg gcgagccgct tcattagatc ctatccgtgc gctacgagct
     1141 gattaagacg ggtcagaaga tgaggaggaa aacgaatgaa ccagttaatg aataatctgt
     1201 tgatgggatt tgatacgctt aaagctcata aactccgaac ttttctgacc atcctggggg
     1261 tcattgttgg aacttccacg gtcattgtca ttgctgcttt tgtttcagga atcaatgtac
     1321 aggtaaccaa ggaaattgaa gcctggggca caaacaccat ttatgtgtat aagttcaacc
     1381 cagggtttaa ctttcgccca acacttgaag aacgtacccg taagccgata accaaaaagg
     1441 aagctgtcgc tattcgcgaa ggatgccctt cggtcaggga agtcgcgctt tttctctctc
     1501 caattgattt ttcaaatgag ggaggccgat tggagcgccg aattccaatt cggtatcagg
     1561 atcatgaaat gcgcaacagt accttacagg ggacgtggcc ggcctatgag cggatgggag
     1621 tcgtcaatct cagtgaaggt cggtttttta ccgaagaaga aaacacccga tgtgaccggg
     1681 tttgtgtcat tgggttggac gttgcagaaa ctctctttcc aacaactgcc gcattaaacc
     1741 gtgaggtgtt gattgataat tatccattca aagtgattgg cgtactggca aagcgcgatc
     1801 atttcctggt cggagaagat gacgcaaaca atgaaaacaa agcggtttac attccttatg
     1861 aaacttgcca caaaatgtat ccgcatctgg acgatcattt tgtgatgggg atggctcaac
     1921 ctggagaact cgatgctgct gtgaatgaaa tgacaaacgt gctcagacgg gtaagaaagg
     1981 tgacgtacaa tgatccagat aattttggga tctcgacatc agataacatt gtcgagcaat
     2041 ttgggcagat cacaggtggt atttttctgc tcatggttgc catttcaagc gttgggttgc
     2101 tcattggcgg tattggggta atgaatatta tgcttgtttc tgtgactgag cgaacgaaag
     2161 aaattggaat tcgcaaagcc attggagcgc gtcaacgaga catcatccag cagtttttga
     2221 ttgaagcggt gactttgact gggattggcg gattgctcgg cataggaatt gggtggttac
     2281 tggcaattgg tatccggtac attctcccat cctatgtgcc acctttagct ccaatcattg
     2341 gaatcgtggt atcggttgcc attggggtta tttttggatt atggccggca ataaaagctg
     2401 cccgacttga cccgattgaa gctctccggt atgagtaata ctttttaggg ctctccgggt
     2461 tgggggcgag gggctggagt gattacggtt ttctcaacaa cctggcctgg gcattagcaa
     2521 aatagacgct tttgaaaaaa ttcaaccacc agtcacctga gacaaaaaag ttgaaggcag
     2581 aatcattatg tcgctgaccg agcaagaaat cattgggtat gcctgcccaa aatgccattc
     2641 cccggttcaa ctggcaccag ataaatcctc atttgtatgc tctcggtgcc aggttgctta
     2701 tccaattcag gaagacattc ctcacctgat ttatgaagag tcaattcctt ggcaataaat
     2761 cttgatacac ttcgacgcat actgctggtt cggttgaggt caattggtga tacggtgttg
     2821 atgactcccg tgctggaagt tttgaagcaa tggtcacctg aaatctctat ttcagttgtc
     2881 agcgaaccgc tttcggcacc ggtgcttgag gcgcacccac tggtcgatca gttgtttact
     2941 gtgcctcgaa ctcgcaacaa gtggcgtgat ttggcgttac ggggacgtct cattcgtgat
     3001 ttgagaaggg agcgctttga tcttgcagtc aacctgcacg gaggaacgac cgccgcctgg
     3061 atctgctggc tttcaggaac gccgcaccgg attgggtttg ctctacccaa tacaaaattc
     3121 ctcttaacca cactcattcc tccgccacag gaagtctggc agaaaaaaac gattcactgt
     3181 gttgagcaac aacttgcctg ggtcaagtat cttgggattc ccattcatca acctccggtc
     3241 ctttcactga actgtgacgc tcaatgtttg aaaacggtcg ccgcttttct gactgataga
     3301 ggtattgcgt cgcactatgc ggtttttcac cccgcagccg cctttacaac caaacaatgg
     3361 gcgccagagc ggtttgcaaa ggtgattgaa tatgtcaaag gattgggatt gaagccgatt
     3421 atttgtgtgg cgaaagcgga ggctggggtc gcacaggcga ttcaacgtag tctggtgtat
     3481 caagatgaaa cgctggttat tgaccacttt tcactttcag aaaccatggc cttgattgct
     3541 ggtgcatcgc tttttgtcgg aaatgacagt ggaccagccc atatcgcagc cgcattgagg
     3601 cggccaatgg tagttgtttt tgggtcttcg aacgaagacg tctggcatcc ctggacgtca
     3661 gcccctcacc agatagtgcg gcaccacatc ccttgtgtcc cttgtgccgg gtacacctgc
     3721 cacgcatttc cagaaccaga atgtattaaa ggagttgagg tgaaggcagt tatcaacgca
     3781 attgacagcg tacttggtcg atagtgcctg tcgggtgtga gaagtcatac aacccacagc
     3841 ctggtcggta ccctttatta gcttggaacc ggcatatctt catcaaccca ttcctgatat
     3901 cgaaccatat cagcgccaga gaagcgtttc aaaagttcag ggcgatccag cagttgcccg
     3961 gcgccaatca cgactgcttc cattgggttc gacgcgcgca tggccggtaa tccaagttca
     4021 agatgaacgc gtcggtaaaa atctcgaagt aaagagccgc ctccagcgag agtgacaccg
     4081 gttcgatgga tatcaaccgc cacggctggt ttacttaact caatcacctg cctgatacct
     4141 tcgataattg tatcgagggt gtgatcaagg gcttccacca cttcgctctg agtcaacatc
     4201 aactcgcgga ccttatagtt ggtcaggttt ttaccggtta ccaacataga tgcttcattt
     4261 tcctgggcaa ttgctgagcc cagattgatt ttgatttgct cagccgattg ttcgtcaatc
     4321 accagtttat gtttccggtg catgtattcc acaatatcct gagtcatgcg attaccggca
     4381 atttcaagtg attttgaagc gataatgcca gtggtactga caatggtgat attggttgtt
     4441 ccaccgccaa tatccaccag aaggtgagcc tgagggcttc gaaacatcac ttcagagccc
     4501 accgccgccg ccagtccttc gtcaattaag aaaactttat tggcgccaat actataggct
     4561 gcattgcgga gtgcccgacg ttcggtttga gtggctgaac tgggaatgct caccaccagt
     4621 ttgcaatacc gccgtgattc acgcttacag gccgcgtgaa agaaattccg aagcatcttt
     4681 tcagccagat catagtcggc aatggtccca tctttcattg gacgatgtac cactgtgtca
     4741 tatggctcgc gacctagcat cgcaatggcg cgtcgcccaa ccgccaccac atttcctgaa
     4801 tcccgattaa tggcaatcgc ggagggttca ttgagaatga tcccatggcc acgggaataa
     4861 atcagggtat tggccgtacc gaggtcgatt gcaaccccgc tgacaaaaaa atttcgaatt
     4921 gattcccacc acatgatcta tcacccttga tgaaaaagct taaaaaggaa gaactcaacc
     4981 gaactgtggg atgcagagca atctggttgt gtttttaaga tgagacaacc ctttttatca
     5041 gaacaggttg cgaattgcaa aggacgaatg aaaaactcaa gctggtcaca gttaaaccgg
     5101 tgaaacagtt ttttcacttg ctgttccaat cggtgtcgtt cgttgatagt attacttact
     5161 actggataaa aatctatatt ccgtgccgat tacgatgccg ttctatctga aagctgtggc
     5221 ccattgagag tttaggacac tggttaaaat aaaatcccgc cagtagtaac cggatgtaga
     5281 tatggtgcca aaaagtgaaa gagaccctac ggaaaagaca gaaagtaaat ccagattctt
     5341 caatgatttc taagacttca aagcattaca ggccaaagaa atagctgtct gaaaagcgat
     5401 agatgtctgg cttggcttca agtttgtcgc tggaagtgag tgtgactgga tcttcacctt
     5461 aaaactcaac catcaaattc caagtcctcg aacacgtcgg gatcgggtga ctggaaaact
     5521 tgaatctaaa cgaaggccgg gctttggtac cgccagtttt gattgggatt cctgacttgg
     5581 aggcgccttg gtggtatctg tttctggttg agtcgtttcg ggttgttctt caaagtgttt
     5641 cagaatttgt gggcgggcct gttttactgc tgagaccaga ccaagataaa cactttgaaa
     5701 ttcatcaaat ccaacctggg gatcagtgcg aacggaaaac ttaaacccaa tatcatcctc
     5761 ttcatcaata aaaaatgctc cgactgccag ttgatcattg aggcttaata acgtcttaaa
     5821 aagtggggat gggttggcca cttttttggt atttccaaat ttgtaggcgt aaaaccccag
     5881 cattttttta gagggcatgt taacgagtac cagatcaacc gtttcttttc cgctctggta
     5941 atgcgtcaca agcgtcgtat tgttcccctg tcgtttttca ctcttcacgt tcatccgttt
     6001 taaatatgac tgcaaggtgt caaccagagt tggcgccggt ttgggggagg ttgaagtgga
     6061 ttggccaggt gctgtgctgg tggcagatgg tgccggtttt ttcttgggag gcggagcctc
     6121 ctgggcaaaa ataggtgatc ctgtcaaaga acagaaaagc aacaacacgg caggtacaaa
     6181 ataggtcaag cgcataaaaa cagtgatgaa cgatttcgag tttggtgtgg tgattggagg
     6241 aattgagggg attgacaggt atggaaaacc caaagcccgc ctgcaaagta gcatgccctg
     6301 gaaaaggctg gcaaggattc ttttcctaac caaaatatgc tcaaatagga tgctgatttc
     6361 accttcacca ggttcatttc tcaatgaaac agtgctggta attgagcctc aggtcgtatc
     6421 tcaaaagtcc tcttccgtac cttctttcaa cccatgtccg aacaaaaacc tcttgtatct
     6481 ccacccgtta aaacacttga aaccgaactg gctgaatttg ctcgtgatca attccggaga
     6541 tttgccccac gcaaagtgct gccgaccgga tttcaagttc agttttatcc ctatgccggg
     6601 tttaacaata cgattcggtt acgacagggg atattgttga ttcggctctc tgatttgctc
     6661 aaaggtgcct caccagtagt tcgacaggca gttattggga tcttgattgc caagattttg
     6721 aaaaagcctg ttcctgccga gtatgaagaa gctttccgag tgtttactca gcttccgagt
     6781 gtaaaaaccg cgattgagcg tgcgcgccgg gtccgagggt ttaaacacgt cagcggagaa
     6841 aagggccacg tctatgatct tggaaaatta tttcaagagt taaaccacca gtatttttcg
     6901 gatgaggtaa gtcagcccac gctgacctgg agccgaaccc aaactcgccg gattttaggg
     6961 cattatgacc gtgcccatca tactatcgtc atcagtcgaa cactggatga tgtccgtgtt
     7021 cctaactatg ttgtggccta tgtgctctat cacgagatgt tgcacatcaa gcacaaggtt
     7081 cgttccgtga atggtcgcaa ctgtattcat tcctctgagt ttcagagaga ggaacgtgtt
     7141 tttgcccact ataccgaagc ccaacagtgg ttggctgaac ttgcccgtcg tcccaaaccc
     7201 aggccagtcc ggagacggtc ccccgccaaa ccaaagtagc gaggaagtag aatgaagaat
     7261 gaagaatgaa gaatgaagaa tgaagaatga agaatgaa
//
LOCUS       DAOQDO010000026         7278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5878_length_7278_cov_3.308044, whole genome shotgun sequence.
ACCESSION   DAOQDO010000026 DAOQDO010000000
VERSION     DAOQDO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5878_length_7278_cov_3.308044"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..798)
                     /locus_tag="PKZ53_00880"
     CDS             complement(340..798)
                     /locus_tag="PKZ53_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39016.1"
                     /translation="MIHHISISAKNTRKAADVLAEVIGGQVVPAPPEFPSNSWFVLAG
                     DAHGTFVEVMPYGTELQPGDGLPNFVPTEPGSPYSASHALISVTATCDQVVQIGQREG
                     WKTERCQRGPFDLIEFWVENRILFEFTTPEMTAQYIGFLTSPNGLQPPLD"
     gene            complement(842..2248)
                     /locus_tag="PKZ53_00885"
     CDS             complement(842..2248)
                     /locus_tag="PKZ53_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNJ39017.1"
                     /translation="MSVSLDISQPAETKLPPGPKGIPFLGSFLDFRRDPLGFLRTAAL
                     EYGDVVYLKFWNQRIYLLNHPDYIRDVLMTGNQNFIKSRGVERTKVFLGKGLLTSEGE
                     FWRRQRRLSQPAFHRQRIAAYGAVMVEKAQQMQARWQEGDRLDLRKEMMRLTLAVVGK
                     TLFDADVEGDAENVGAALNVIEQNFNRLLLPFAEWLDKLPLPSNLRLWRAIETLDAVI
                     YRLISERRSTGKDHGDLLSMLVFARDEEGDGTGMDDRQLRDEAMTIFLAGHGTTAHAL
                     TWTWYLLSQNPAAESALHAELDQVLKGRVPTFEDLPNLRYTEMVFNEAMRMYPPAWAL
                     GRRTIDGFDVGGYHIPAGATIVLSPWVMHHDPRYFPDPFHFDPQRWTPEARAERPKFS
                     FFPFGGGSRVCIGESFARMEGVLLIATLAQQWRLQLVPHQTVEPQAIIALQPKNELWM
                     TAVGNGNRRFSEKECGAR"
     gene            complement(2331..3701)
                     /locus_tag="PKZ53_00890"
     CDS             complement(2331..3701)
                     /locus_tag="PKZ53_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ39018.1"
                     /translation="MKFKSPPSSLFFIVLILFTLMTTATGAHPAPQPVRHIPPDPTLV
                     AEIERIKAIDHHAHPMRLVKEGEWDNESDALTLEGIEPFSTPVRLRPDNPEYIAAWKA
                     LYGYPYTDATEAHVRELKELKRQAMSNQGDRYPAWVLDQLGIETMFANRVAMGRGLQA
                     PRFRWVSFIDALLFPLSTKAARNINSDYRSFYPDEERILKNYLTSLGIKSLPATLGNY
                     LATVVTPTLEQQKRDGVVGVKFEAAYLRTLDFADADEAEASRIYAKYVRGGEPPTAGY
                     KVLQDYLFRSIAREAGRLGLAVHIHTGIGIGAYFNVNGSNPLLLESALNDPTLRKTNF
                     VLVHGGWPFATQTAALLSKPNVYADFSAQTFLRQPHELSRVLRQWLEFIPEKVLFGTD
                     AFGLTPEIGWEEVGWLTTTSSRKALALALTGMMNDGEITRDQAVKLARMALRENAINL
                     YGLKTE"
     gene            complement(3785..5485)
                     /locus_tag="PKZ53_00895"
     CDS             complement(3785..5485)
                     /locus_tag="PKZ53_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39019.1"
                     /translation="MKSGIFLLFMFLAGSLPFLAQDNPPKSHCLSAKPEPPLPTSGRA
                     PRLMTGIGKASLTITTKSPRVQRYFNQGLALLHCFWGNEARRSFQEAIRLDPNCAMAY
                     WGIYQVMSNPFTGGANPEEKKEALDKAVALSSGGSPHEQAYIKAIETREALGGKKGRL
                     AYHQAMESLIADYPNDLQAKLFLAQFLFVYDFSYTFNLTGEPSKNRQRSLELIKEVLA
                     TDPNNAAAHHYWIHVVEGTNHPESALNSARILSHLAPASGHMVHMPGHIYFRLGNYEL
                     ARHAFLASLKVEKDYFIQNRINPELLWNYSHNLSFLAMNCAEDGRYREGLKWSRLGIP
                     DTDPGIFNLRYAFWDKAVREFEDPGFIAANAEESPFIKNFYRGLIAFCKGMASIDAGD
                     SAEATRQAESLDQTLMAMNATTLSEEEAYDLEFLPKILAVALPELQGSIKGLQGDLTG
                     AVTLLHAALNQEQAIGYEEPPPYPRPVFESLGQIYLRAKEWERAREAFQQVLKMRPNS
                     GHAFFGIAQSYDLAGQKAEAVVAYKIFLKSWRKADPDLPQVKTAVARLKQYRAEISLH
                     "
     gene            6073..6636
                     /locus_tag="PKZ53_00900"
     CDS             6073..6636
                     /locus_tag="PKZ53_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020888315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39020.1"
                     /translation="MSVNKEDCILFSGGANGAEAEFGAVAEAFGIEEVNFTFEGHNIA
                     RQRGLRVLNHEELKNGDVSLEYISRLMNRRYTDSPVFRKLLQSIWYQINNGQEIYVIG
                     EILEDKTVKGGTGWGAEFAKLCNKPLHVFDQTRDAWFTWNQVEWVECTADTLPVITHV
                     HFSGTGTRLLEDNSRKAIRELFERSFS"
     gene            complement(6806..>7278)
                     /locus_tag="PKZ53_00905"
     CDS             complement(6806..>7278)
                     /locus_tag="PKZ53_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNJ39021.1"
                     /translation="RIEERNLSSQPYYRSDIGAHKVKILANSLFRALGVQLDSVTDRL
                     TNDNARKLLHGSQIVVDTFDNSVSRAAVKQACETYGIACLHAGMASDFAEITWNEQYR
                     VPSATQDDVCEYPLARNLVMLTVAVTSETLIHFIESREKNGFMITLRDLNVKQV"
BASE COUNT         1810 a         1773 c         1824 g         1871 t
ORIGIN      
        1 gatttacagt gttgtcagac attgaaagat gctccattta tttgagatat gtcagaaatg
       61 agattggggg gtaaggttgg gaaacacatt aaaatgtgaa cctggatttg agtcaatccc
      121 aaaaacaaaa tccaggaacg tttgtccttc ccaccagaaa acctgcgatc tgaattgagt
      181 tcggcgtatt tcttgccgtc aaatgcctga tgtgattttc aaaattgtga acgcgttcac
      241 aacaggttaa agcatagaat caaatacagt taaatggtcc taagctgctc atgtccatca
      301 acccgaaaga gaactttaga gttggtgtgg ttcaaattct caatcaagtg ggggttgaag
      361 cccgtttgga ctggttaaaa atccgatgta ctgggctgtc atttccggtg tggtgaactc
      421 aaacaaaatt cggttctcaa cccaaaattc aatcaaatca aacggcccac gctggcaccg
      481 ctcggttttc cagccttcac gctggccaat ctgtacaact tgatcacagg tggccgtcac
      541 tgaaatcagg gcatgactgg ctgaataggg tgagcccggc tcggttggga caaaatttgg
      601 aagcccatct ccgggctgga gttccgttcc atatggcatc acttcaacaa acgtgccatg
      661 cgcatcccca gccagtacaa accaggaatt ggatggaaac tcaggcggtg ctggaactac
      721 ttgaccacca ataacttctg ccagaacatc agccgctttc cgggtatttt ttgctgaaat
      781 cgaaatatga tgaatcatcg ttgcactcct taaacttgtt gtataccaaa actttgacaa
      841 gtcaccgtgc tccacattct ttttctgaga agcgacggtt cccgttgcca actgccgtca
      901 tccagagttc atttttgggc tgcaaggcaa taattgcctg gggttcaacg gtttgatgtg
      961 gaaccaactg aagccgccac tgttgggcca gagttgcaat gagcagcacc ccttccattc
     1021 gcgcaaagct ctcgccgata cagacgcgtg agccgccacc aaacggaaaa aaggaaaact
     1081 ttgggcgttc ggctctggct tcaggtgtcc atcgttgagg gtcgaaatgg aatggatcag
     1141 gaaagtaccg tgggtcgtgg tgcatcaccc agggactgag cacgatggtg gcgccagccg
     1201 gaatatgata gccccccacg tcaaacccat caattgtccg tcggcccaat gcccaggctg
     1261 gtggatacat ccgcatggcc tcattgaaaa ccatttcggt gtatcgcagg ttgggcaaat
     1321 cttcgaatgt tggaactctg cccttgagga cctggtcaag ctcggcgtga agtgctgact
     1381 cagccgccgg gttttgcgac agcaaatacc aggtccaggt cagtgcatga gctgttgttc
     1441 cgtgcccggc caggaaaatt gtcattgctt cgtctcgcaa ctggcggtcg tccattccgg
     1501 tgccgtcccc ttcttcatca cgggcaaaga ccagcatcga gagcaagtcc ccatggtctt
     1561 tgccagttga ccgccgttcc gaaatcaggc gatagatcac cgcgtctagc gtctcgattg
     1621 cccgccagag ccgaagattg gaaggtaacg gcaatttgtc gagccattca gcaaacggca
     1681 gcaacagccg gttgaaattc tgctcaatga cattcaacgc agcgccaaca ttttcggcat
     1741 cgccttcaac atcagcgtca aagagggttt tgccgacgac cgccagtgtc agtcgcatca
     1801 tttctttcct caggtcgagc cggtcacctt cctgccatcg ggcttgcatc tgttgtgcct
     1861 tttcaaccat caccgctccg tacgcggcaa tgcgttggcg gtgaaaagct ggttgcgaaa
     1921 gtcggcgttg acgtcgccag aattcacctt cactggttaa taaccctttt cccagaaaca
     1981 cttttgtgcg ctcaacacca cgacttttga taaaattctg gttaccagtc atcaacacat
     2041 cgcgaatgta atcaggatgg ttgaggagat atatccgctg gttccaaaat ttcagataca
     2101 caacatcgcc atactcaagc gccgcggttc tcaggaaacc gagcggatca cggcgaaaat
     2161 ctaaaaaact ccccaaaaat gggatccctt ttggtcctgg aggaagcttc gtctcggctg
     2221 gttgggaaat gtccagggat acactcattg tgaacgtctc cttttgtctc acgtaacaag
     2281 gccagttgtt gccataagcg gtatttcaaa gactgcatcc gcatcaagag ttattcggtc
     2341 ttgagtccat acagattgat cgcgttttca cgcagtgcca tgcgtgccag ctttaccgcc
     2401 tggtctcgtg tgatttcgcc atcgttcatc atgccggtga gggccagtgc cagtgctttt
     2461 ctgctactcg tggtggtaag ccagcctact tcttcccatc caatttccgg ggtcaggcca
     2521 aacgcatctg tgccaaacaa cactttttcc gggataaact ccagccattg acggagcacc
     2581 cggctcaatt cgtgcggttg acggagaaat gtctgggcgg aaaaatctgc atagacgttc
     2641 ggttttgaaa gtaaggcggc tgtttgtgtc gcaaagggcc agccgccatg caccagcaca
     2701 aaatttgttt tgcgcaaagt cggatcattg agagcagact caagcaagag cggattggat
     2761 ccgttcacat tgaaataggc accaatccca attccggtgt gaatatggac ggcaagacca
     2821 aggcggcctg cctctcgtgc aatggaacgg aaaagataat cttgcagcac tttgtacccg
     2881 gcagtcggtg gctcaccacc tctgacatac tttgcataga tcctgctggc ttcagcttca
     2941 tcggcatcgg caaagtccag ggtacgcaga tacgccgctt caaacttgac ccctacgaca
     3001 ccgtcgcgtt tttgctgttc cagcgtgggc gtcacaaccg ttgccagata attccccaat
     3061 gtcgccggca atgattttat tcccagactg gtaaggtagt tttttaaaat gcgctcttca
     3121 tcggggtaga aagaacgata atcggaattg atatttcgcg ccgctttggt gctcagaggg
     3181 aacagtaagg catcaataaa actgacccac ctgaaacgtg gtgcttgaag accccgcccc
     3241 attgccaccc ggttggcaaa catggtttca atccccaact ggtcaagcac ccaggctgga
     3301 tacctatcac cctggttgct cattgcctgt cgtttcaatt ccttcaattc acgcacatgc
     3361 gcttcagtcg catcggtgta aggatatcca tagagcgcct tccaggctgc aatatattcg
     3421 gggttatccg gacgcaagcg gacaggtgtg gaaaacggtt caatcccctc aagcgtgagc
     3481 gcgtctgatt cattgtccca ctcgccttcc ttcaccagcc gcatcggatg ggcatggtgg
     3541 tcaatcgctt ttattcgttc gatttcagca accagggttg gatcaggtgg tatatgtcga
     3601 actggttgag gtgcaggatg agcaccggtt gcagttgtca tcagagtgaa caggatgagc
     3661 acaataaaaa agaggctgga gggaggagac ttgaatttca tgacacgatt cctataagcc
     3721 attgagagat tttatgaagt aagaaatgtt ttgtgacggc gtctgggttc cgggttttcg
     3781 ctcttcaatg aagggaaatt tctgcccggt actgttttaa ccgggcgaca gcggttttca
     3841 cctgtggtaa gtcgggatct gctttccgcc agcttttgag gaatattttg tacgccacga
     3901 cggcttccgc tttttgtcca gccagatcgt aggattgggc aatcccaaag aaggcatgcc
     3961 cgctgtttgg gcgcattttg agaacctgtt gaaatgcttc gcgtgccctc tcccactctt
     4021 ttgcccggag gtaaatctgt cccagacttt caaagaccgg tcgtggatac ggcggaggtt
     4081 cttcgtatcc aatcgcctgc tcctggttga gtgctgcatg taacagggta acggcaccgg
     4141 ttaaatctcc ctgaaggcct ttgattgagc cttgcaattc agggagcgcc acagccaaaa
     4201 ttttaggtag aaattccagg tcgtacgctt cttcttcgga aagggtggtt gcgttcatcg
     4261 ccatcagcgt ttgatccaaa ctctctgcct gtcgggtagc ttcagcacta tcacccgcat
     4321 caatcgaagc catcccttta cagaaggcaa tcagtccacg atagaaattt tttataaatg
     4381 gggattcttc agcgttggca gcgataaatc ctggatcttc aaactcacga accgctttgt
     4441 cccaaaaagc atagcgaaga ttaaatatcc ctggatccgt gtctggaatt cccaaccgtg
     4501 accatttcaa gccttcacga taccgaccat cttcggcaca gttcattgcg aggaatgaca
     4561 agttgtgtga gtaattccac agcaactccg ggttgatccg attttgaata aagtagtctt
     4621 tttccacttt cagcgaagcc aaaaaagcgt gacgagccag ttcatagttg ccgagtcgga
     4681 agtaaatatg gccaggcata tgaaccatat gacctgaagc tggtgccagg tgactgagta
     4741 ttctggcact gttgagtgct gactcaggat ggttggtgcc ttccacaaca tgaatccagt
     4801 aatgatgggc ggctgcattg ttggggtcgg tggcaaggac ttctttgatc agttcaaggc
     4861 tccgctgacg attttttgaa ggctccccag tgagattgaa ggtataggaa aaatcataga
     4921 caaataaaaa ttgtgccaga aacagtttgg cttgaaggtc atttggataa tcagcaatca
     4981 aagattccat cgcctgatgg taggccagtc ggcctttttt accaccgagt gcttcacgag
     5041 tttcaattgc tttaatgtat gcctgttcgt ggggagagcc acctgaactg agggcaactg
     5101 ctttatccaa agcttctttt ttttcttccg ggttagcgcc gccggtgaaa gggttggaca
     5161 taacctgata tatgccccaa tacgccatgg cacaattcgg gtcaagtcga attgcttctt
     5221 gaaaagaacg cctggcttcg ttgccccaaa aacagtgcag gagtgccaat ccctggttga
     5281 agtatcgctg aacgcgaggt gatttggtgg taatggtgag ggaagctttc ccaattccag
     5341 tcatcagacg tggcgcacga cccgatgtgg gcaatggtgg ttctggtttg gccgaaaggc
     5401 agtgactttt tggaggattg tcttgcgcca ggaatggaag tgaaccagca agaaacataa
     5461 ataaaaggaa aattcccgat ttcataccct tactcaagaa aagaagattc ccggttgaga
     5521 gaattctgtt gaccaaaatt ctcaaccaac tggatggatg cgaaccacat tggataggag
     5581 tttgtgacaa agcgaagtga tttggagtgt gaatggagtc aggggaggct acgacgcggg
     5641 ttccaaaaac ggttttcgaa atcttggggt gttcaggttt ttcctggtgt tggtttcaga
     5701 accaatcaga acatcactca atcattcaaa actgcccgaa gtgaggatcg ggtatcctgg
     5761 aggttaattt gcaaatggag gttttttgaa atttgtgaac gcgttcacaa cgggtcaagt
     5821 aattgaaaag tcgaggttta ggtggtccta tggtagaaag ttgtgattgg tttcgacttc
     5881 ctgaaatatg tgaggggatt gtgtattcgc ttaccaactc cagctctcac cagttcagcc
     5941 catctgtgct tctttttccc tttttgcttc aaagaattag gctggctgtg gtagcttagc
     6001 ccggtttctt tttaccatcc gctgtacttc attcagcatc attccaaaaa aggcgaggat
     6061 tcgaagatta tcatgagcgt gaataaagaa gattgcattc tgtttagcgg cggtgccaac
     6121 ggtgccgaag ctgaatttgg tgcggttgcc gaagccttcg gcatcgaaga agtcaatttt
     6181 acctttgaag gccacaacat tgcccgccag cgtggtctgc gggttctcaa ccacgaagag
     6241 ttaaaaaacg gagacgtcag cctcgaatac atttcgcggt tgatgaatcg ccgttacacc
     6301 gacagcccgg ttttccgcaa attgttacaa agcatctggt atcagattaa caatggccag
     6361 gaaatctatg tcattggcga gattttggaa gacaaaaccg tcaagggcgg cactggctgg
     6421 ggcgctgaat ttgccaagtt gtgcaacaag ccgctgcatg tgtttgatca aacacgcgat
     6481 gcctggttta cctggaatca ggttgaatgg gttgaatgca cagccgatac cttgcccgtg
     6541 attacccatg tacatttttc cggcactgga acgcggctgc ttgaagacaa cagccgcaaa
     6601 gccatccggg aattgtttga acgatcattt tcctaaaaaa cagggcggaa aaacccaggg
     6661 ctcagggctg aagaagtcgg gctgaagata ttgggcttgg ggctgaagac tcgcaagctc
     6721 ggggctgaag aaaacggatt tcataccctg agcccgttgt cttcagcccg tttttttcag
     6781 ccctgagccc tgggtttttc cgccctcaaa cttgtttcac attcaaatcg cgcaatgtga
     6841 tcataaatcc attcttttcc cgactttcaa taaaatgaat cagtgtttct gatgtaacgg
     6901 ccaccgtcaa catcaccaaa ttgcgagcca gtggatactc acacacgtca tcctgggtcg
     6961 ctgacggcac ccggtactgt tcattccagg taatttcggc aaagtcagaa gccataccgg
     7021 catgaagaca ggcgattcca taggtttcgc acgcctgttt gactgctgcc cgtgacacgc
     7081 tgttgtcaaa cgtatccacc acaatctggc ttccgtgcag gagttttcga gcgttgtcat
     7141 tggtcaatcg gtcagtgacc gaatcaagct ggactccgag cgctcgaaaa agagaattgg
     7201 ccaggatttt caccttgtgc gccccaatgt cgcttcggta gtaaggttgt gacgataaat
     7261 tccgctcttc gatgcgat
//
LOCUS       DAOQDO010000027         7227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5922_length_7227_cov_2.975878, whole genome shotgun sequence.
ACCESSION   DAOQDO010000027 DAOQDO010000000
VERSION     DAOQDO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5922_length_7227_cov_2.975878"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..172)
                     /locus_tag="PKZ53_00910"
                     /pseudo
     CDS             complement(1..172)
                     /locus_tag="PKZ53_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
     gene            complement(716..3601)
                     /locus_tag="PKZ53_00915"
     CDS             complement(716..3601)
                     /locus_tag="PKZ53_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNJ39022.1"
                     /translation="MNKEMIISASAHEKRVAILENGVVMEFYVERIDETQAVVGNVYK
                     GRVVKVLPGMQSAFVDIGLERDAFLYVSDFFEEEGNDEYDTESVSTEGQSRGKGRDGR
                     DSQRQQSDRDLSGRHKRERDGRSPLPEPAESTAKAKPVIEDQIEFDSRLLPPDLDVDD
                     SLSEVSMITDALDLSVSPEAIVVELDPEELQTVLDAESEVQLERVELEPEEEEPVVAE
                     PVATEEPDLDLALALSDGFQFERVTDHDVPHVEPEPPAPESEVMVDAPEISPESVSVA
                     EIQPVAVEAEAKPQGRRRGRKKAEPAKVEPPPVEVPVQVEPAPVEPTGKKKQTRGRKK
                     AVPAPALEFERILDDDVLSEAGDLLKEAIVQHKILEQTRQAEYETAPVLAGDEEATPT
                     AVEAASPATEVVRPAPVRVADEEPATEEVVSDADLTDSEASIGKRSRNSEFASRRGRR
                     GRRRGRPATGGGPGQADEETGEPVLSDSDVAVLLGDEAESDGADSETPDLKLKDDSLG
                     DDSAVPPQPPVAVPPPARREPRDLPPPRDTRGKSDARPPREFRGRAPGITDLLREGQE
                     ILVQIAKEPIGTKGARITSYVALPGRYLVYMPTVAHIGVSRKIPSDQERTRLKRTMIN
                     LRDRDQIPGGFIVRTACEGHTEEELREDMMYLFRTWQDMRKRSEHSKPGTVVYRELDL
                     VQRLLRDHLSNEFSVIRVDNEEEYARVVDFISRFQPKLLDRVKLYTRKRQIFEEYGVQ
                     TELDAALKPRVWLKSGGFIVINQTEALVAIDVNTGKFVGRSNRLEDTIVKTNLEAAVE
                     IVRQIRLRDLGGIIVLDFIDMEERKNRQKVMQVLEQEIKSDRSPSKILQFNDFGLVAI
                     TRKRVRQSLERILCEPCPYCTGAGMVKSAETVCYEILGEARRMASDMEGETRSREVTL
                     RINPSVAKALRNEQKQVWGEIEDYLGCSVSLQSDPNIHQEHYDFAFL"
     gene            complement(4547..5668)
                     /gene="rodA"
                     /locus_tag="PKZ53_00920"
     CDS             complement(4547..5668)
                     /gene="rodA"
                     /locus_tag="PKZ53_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNJ39023.1"
                     /translation="MMTRDIRDFDWPLLIAALVLCGLGITGIYSAQPEGNFWLKQLIM
                     VGVGLVVMFVLSQINFQRLFDVVPYFYAVTMILLVLVLVIGTKINGQRCWISLGPLGT
                     LQPSEFAKLGTIMMVARILHPLQRRAVTLDFALKACAAVVLPVGLIMLEPDTGTALTF
                     FPVLVAMLFVSGLNRRLIITALVLALIVAPFGFKYVLEPRLKQYQRDRINVAINAMFA
                     PEKLQGREIREGFGYQTLQSMIAVGSGGVTGKGITKGTQSQGGFVPEKRTDFIASVLA
                     EELGFAGSIFMLALLLFIIIHSARTAERARDRFGLLILTGFVTLLSFHAVINIGMVVG
                     LVPIMGIPLPLLSYGGTSLLMTFMCLGIVANVYQQRFVN"
     gene            complement(5702..>7227)
                     /gene="mrdA"
                     /locus_tag="PKZ53_00925"
     CDS             complement(5702..>7227)
                     /gene="mrdA"
                     /locus_tag="PKZ53_00925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNJ39024.1"
                     /translation="LGDIASHILGYVHEISPSQLKAPIRAEYENCKPGDKVGQAGLEK
                     SYNQVLMGKEGYRRVIVDSRGRFVSELEQVPPSPGQDVVTTIDLDLQLMAEQVLRERG
                     LDGIIVVMDPRNGEILAMASFPGYDPNLFSSGISKQDYAKLAQDPHKPLRNRAIQDIY
                     PPGSTWKLIMAVAGMKSDLLDPDMTYVCGGGITVGNKFTRCMGAHGAPKIDYALTKSC
                     DGFFYRLGIKLGLENLQKWATEMGCGQYTGIDLPNEFKGYIPSLELKAATVRRSFPNP
                     TAREFEWRDIDSVYASIGQAMVRPTPLQMLRAAAGISQRGTFHTPHLLLEARPTKDYP
                     ARFFADKVHKIEMPAENWEAIVKGMWGAVNAGGTATSSAITEFPGWQMCGKTGTAQVV
                     SKIKTEKLEEKDHSWFVAFGPRENPEIAGISLVEHGGFGAKASAPNVKAVFETYLKKK
                     NGMPIEVSLETVQAAAKTAEKFRKAWQDTIARRQQKTETRATKPATTTKPAATAPVQ"
BASE COUNT         1712 a         2040 c         1766 g         1709 t
ORIGIN      
        1 tgcgagcgcc gatcttctgc cattcgtaga agccgctcat gggcacgacg caccggcgtg
       61 atcggaacgc ctcgcgaaag gccggcaatc gcgaggcggt ttcgctgcgg gcgttgatgg
      121 tgcgttgggc gatggactcg tctttcgccc acgacgggat gagcccccac cgccaggcgc
      181 ggcgccccaa tgatttgaat gtccccgctt tccaaatcgg aaatattggt caactcaacc
      241 ccgcactggt tgcaccgcac gtccactgaa gtattcccga catatccaca ttcgatgcag
      301 atccgcatat gaagcttccg cccttgatca caaacccggt tgctggcttt cctccccatt
      361 ctctttcaac ctcccctcaa ttgtctattc ccagctcaga atttgtccaa attaaaacca
      421 atgaataaac tctgggaata gccgaactga caggcaatgg ggacttctgg aaccaaatag
      481 acgggggaaa gagctggcaa ttacttttca gaatgaaaat ttgtgtgtac tcacactggc
      541 aagaattttg gtgggcgagt gattcaagat tccatcactc aataaggtga gtagaacagg
      601 gagcaccgag caaaagagtg aaatagtgaa atcctccgta gtctatttct actttgcgat
      661 ttcactactt cacgattcgc gatcttacca cttcgctcag gatctctgtc ccagtttaca
      721 ggaaagcaaa gtcatagtgt tcctggtgga tatttggatc tgactgcaaa gagaccgagc
      781 agccaaggta gtcttcaatt tctccccaga cctgcttttg ctcattgcgt aacgctttgg
      841 caacagaagg gttgatccgc agggtaactt cgcggctccg ggtttcgcct tccatatccg
      901 atgccatccg ccgggcttca cccaaaatct catagcagac cgtctcggct gatttgacca
      961 ttcccgcccc cgtgcaataa gggcagggct cgcacaaaat ccgttccaga ctctgtcgaa
     1021 cccgcttacg ggtaatggcc accaggccaa aatcgttaaa ttgaagaatc ttggaaggtg
     1081 accggtcaga tttaatttcc tgctccagca cctgcatcac tttttggcga tttttgcgct
     1141 cttccatatc aatgaagtcg agcacaatga tgccacccag gtcacgcagt cgaatctgac
     1201 gcacaatttc aaccgcggct tcgagattgg ttttgacgat ggtgtcttcc agccggtttg
     1261 aacgtccgac aaatttgccg gtgttgacgt caattgccac cagggcttcg gtctggttaa
     1321 tcacaataaa cccacctgat ttgagccata ccctgggctt gagcgccgcg tcgagttcgg
     1381 tctgaacccc atactcttca aaaatctgac gtttccgggt atagagcttg acccggtcaa
     1441 ggagctttgg ctggaagcgg ctgatgaagt caaccacccg ggcatactcc tcttcattat
     1501 ccacccggat caccgaaaac tcattggaca ggtgatcccg caaaagccgc tgcaccagat
     1561 cgagttcacg atagacgacg gttccgggtt tggaatgctc agatcgcttg cgcatatcct
     1621 gccaggtgcg gaacaggtac atcatatctt cgcgcaattc ttcctcggta tgcccttcac
     1681 aggcagtgcg cacaataaag ccgccaggaa tctgatcacg gtcccgcagg ttgatcatgg
     1741 ttcgcttcaa tcgggtccgt tcctggtcag aaggaatttt gcgtgagaca ccaatgtggg
     1801 ccaccgttgg catatacacc aggtaccgac ccggcaaggc aacataggag gtaatccggg
     1861 cccctttggt gccaatcggt tctttggcaa tctggaccag aatttcctgg ccttcgcgca
     1921 gcaaatcagt aattcccggt gcccggcctc gaaattcacg aggtggacgg gcatcgcttt
     1981 tgccgcgtgt gtctcgcggc ggcggcaggt cgcgtggctc ccgacgggca ggagggggca
     2041 ccgcaactgg aggctggggc ggcaccgccg aatcatctcc caggctatcg tcttttaact
     2101 tcagatctgg agtctctgag tcggcaccat cagattcagc ttcatcacca agtaacactg
     2161 caacatctga gtcgctcagc acgggctcgc cggtttcctc atctgcctgg ccagggccgc
     2221 ctccggtggc tggccgcccg cgacgacgac cacgacgacc acggcgcgag gcaaattcag
     2281 aattgcgcga ccgcttccca atactggctt ctgaatcagt caaatcggcg tctgaaacca
     2341 cctcttcggt ggcaggttct tcatctgcta ctcgcaccgg agcgggtcga accacttcag
     2401 tcgccggtga ggccgcctca actgctgtcg gggttgcttc ttcatcacca gccaggacgg
     2461 gtgccgtttc atattcagcc tgacgggttt gctccaggat tttatgctgg acaatcgctt
     2521 ccttgagcaa atcgccagct tcagacaaca cgtcgtcatc caggatccgc tcaaattcga
     2581 gggccggggc aggcacggct ttcttgcggc cacgggtttg cttcttcttc ccagtcggct
     2641 caaccggtgc cggctcaacc tgaacgggga cttcgacggg tggtggttca acttttgcag
     2701 gttcggcttt tttacgccca cgccgacggc cctggggttt ggcctcggct tcgactgcca
     2761 ctggctggat ctcggccaca gataccgact ccggtgaaat ttcgggagcg tcaaccatca
     2821 cctcagattc aggggctggt ggttccggtt cgacgtgcgg cacatcgtga tcggtgactc
     2881 gttcaaactg gaatccatcc gacagggcaa gcgccagatc aagatccggt tcttcggtgg
     2941 cgacaggttc agccacaacc ggttcttctt cttcaggctc aagctcaacc cgctcaagct
     3001 ggacttcgct ttcagcgtcc agcacggtct gcagttcctc agggtcaagc tcaaccacaa
     3061 tggcttctgg gcttacactt aaatccagag catctgtgat cattgaaact tctgacagag
     3121 agtcatctac atccagatct ggcggaagca ggcgactatc aaattcgatc tggtcttcaa
     3181 ttaccggttt ggccttcgcg gtactttcgg cgggttcggg cagtggtgag cgaccatccc
     3241 gctcccgttt gtgacgacct gacaggtcac gatctgattg ttgacgttga gaatcgcgtc
     3301 cgtcacggcc tttaccgcgt gactggcctt cagtggagac cgattcggtg tcgtattcat
     3361 catttccttc ttcttcaaag aagtctgaaa catagagaaa cgcatcccgc tccaggccga
     3421 tatccacaaa tgcagattgc atgcctggta gcactttcac cacacgtcct ttatagacat
     3481 ttccgacgac ggcctgcgtt tcatcaattc gctccacgta aaattccatc accactccgt
     3541 tttcaagaat ggccacgcgc ttttcgtggg cgcttgcact gataatcatt tctttattca
     3601 tagatttcct ctaagcccta caaaatcaat cttttgtcag gtaacttgcg cgttggggac
     3661 tacttcctga caacgaatat ggattatttc aaagtgtgtg gcctggagaa tggtctggca
     3721 aaaaagaagc ggggggagat caagaggaaa actgaaggtg atataccatc aacacctggt
     3781 ggtcaaatct atccccgacc gggcagggat ctgattcttg caaaccacgt gcagggtgac
     3841 gattgaacgc accagaatgc gcccaaacga tatcccaatc aagtcgctga agagagcgct
     3901 ggcagcaaca cttttcaaat caaccggaga tatggaacgg agaacgatca gaagaagggt
     3961 gaacaaccgg tgtgccactg agagcgcaag ccagacttgc gtcaagatac ctgaagcgca
     4021 gtgtcttctc tcaggctggc aaatcctgtg ttttctctca ccagatggtg attttttcgg
     4081 tttcaagcac tatgtcttca gaaaacgaac aggatcttag aagaacgact gacccctggt
     4141 tcaaaacaag agtcaatcca ataccacacc ttatttctgg atgaaaacca atcccaaagt
     4201 tgtttcaacc tgctgccatt gattgacagc aaagtccaaa cgggctgtgt caagcgttgt
     4261 tgctggccat ttttcagcct ctgtttttct caaacagact tcttgcttac cgaaaccaag
     4321 cctcaacaca aaaaagttgg aacgcgagtt gtacactgcc tgacgttcac acttcaattc
     4381 gtcattgatt catcaacact gacaccaccc cctcgaccag agtgaatcct ttggtgactg
     4441 gatctggctc gaaccgcggt cagaacaacc tttccgcctt ccaaacgcca gtcgcagatc
     4501 catccggcac gtgctgcgtg atgggttcag cgtttcaggg tcttgactaa ttgacaaacc
     4561 gctgctgata cacattggca acgatgccaa gacacataaa cgtcatcagc agcgaggttc
     4621 cgccatagct caacaatgga agcggaatcc ccataatcgg gacgagacca acaaccatgc
     4681 cgatattgat gacagcgtga aatgaaagca aagtgacgaa tccggtgagt atcagcaatc
     4741 caaaccgatc gcgtgcgcgt tcggcggtgc gagccgagtg tattatgatg aacagcaaaa
     4801 gcgcaagcat gaagatactt ccagcaaacc caagttcctc agccaaaacc gacgcaataa
     4861 agtcagtgcg tttttccgga acgaagccgc cctggctctg agtccctttg gtaatccctt
     4921 tcccggtaac cccgcctgaa cccaccgcaa tcattgattg gagcgtctgg tagccaaacc
     4981 cttcccggat ttcccggccc tggagttttt ccggggcaaa catggcatta atcgccacat
     5041 tgattcgatc ccgctgatac tgcttcaatc ggggttcaag gacatatttg aagccaaacg
     5101 gggcaacgat taacgccagt accagggccg taatgatcaa ccgccggttg agcccgctga
     5161 caaacaacat tgccaccaac accggaaaaa aagtcagcgc cgtgccggta tctggctcaa
     5221 gcataatcaa tccaaccggc aaaaccaccg ccgcacaggc tttcagtgca aaatccaggg
     5281 tcaccgcccg gcgctgcagg gggtgcaaaa tccgagccac catcataatc gtcccgagtt
     5341 tggcaaattc tgatggctgt aaagttccga gcggccccag actgatccag cagcgctgtc
     5401 cgttgatttt ggtgccaatc accagcacca gcaccagcag gatcatcgtt acggcgtaga
     5461 aatagggcac cacgtcaaac aatcgctgaa aattgatctg cgacagaaca aacatgacca
     5521 ccagcccaac accaaccatt atcaattgct tcagccagaa atttccttct ggctgggcac
     5581 tgtaaatgcc ggtaatcccc aggccacaca acaccagcgc agcaatcaac aacggccagt
     5641 caaaatctcg aatatcacgt gtcatcatag ggtgggtcac cgcggtccag gaagaaggag
     5701 ttcattgtac cggagcggtc gctgccggtt tggttgtcgt tgccggtttg gtcgctcggg
     5761 tttcagtttt ttgttgccgg cgggcaatgg tgtcttgcca ggctttgcgg aatttttcgg
     5821 cggtcttggc cgcggcctga acggtttcaa gtgacacttc aattggcatc ccgttctttt
     5881 tcttcaaata tgtttcaaaa accgctttga catttggagc tgacgctttg gcaccaaatc
     5941 cgccatgttc gaccagtgaa atacccgcaa tttcgggatt ttcacgcggc ccaaaggcca
     6001 caaaccacga gtggtctttt tcttcgagtt tttcggtttt gattttactc accacctggg
     6061 ccgtaccggt tttcccgcac atttgccagc ctggaaattc ggtaatggcc gaactggtcg
     6121 ccgttccacc agcattgacg gccccccaca tacctttgac aatcgcctcc cagttttcgg
     6181 ctggcatctc aattttatgg actttgtcag caaaaaaccg ggctggatag tctttggtcg
     6241 gtctggcttc gagcagtaaa tgtggggtat gaaacgtccc gcgttgagaa ataccggcgg
     6301 cggcccgcag catctggagc ggcgtcggtc gaaccatcgc ctgtccaatc gaggcataga
     6361 ccgaatcaat atcacgccat tcaaattctc tggccgttgg attggggaaa gaacgtcgca
     6421 cggtggcggc tttcaattcc agacttggaa tgtacccctt aaactcattc ggtaagtcaa
     6481 tccccgtata ctggccacac cccatttcgg tggcccattt ttgaaggttt tcaagtccca
     6541 gcttgattcc aaggcgataa aagaacccat cgcaggattt ggtcagagca tagtcaattt
     6601 tgggggcgcc gtgagccccc atacaccggg taaatttatt gcccacggtg attccgccgc
     6661 cacagacata ggtcatatct ggatccagga ggtctgattt catccccgcc accgccataa
     6721 tcagcttcca ggttgaccca ggcggataaa tatcctgaat ggcacggttc cgaagcggtt
     6781 tatgtggatc ctgagccagc ttggcataat cctgtttgga aattccagat gaaaacagat
     6841 tggggtcata tcctggaaat gaagccatcg ccagaatttc gccattgcgt ggatccatca
     6901 cgacgatgat cccatccaac ccacgctcac gcaaaacctg ctcggccatc aattgaagat
     6961 caaggtctat tgtggtgacc acatcctggc ccggtgacgg tggcacctgt tccagttcac
     7021 tcacaaatcg ccctcgactg tccacaatca cccggcgata cccttccttg cccatcaaga
     7081 cctgattgta agatttttcc agcccggcct gcccgacttt atccccaggt ttgcaatttt
     7141 catattcagc tcgaatgggg gctttcaact gggaggggct gatctcatgc acatagccca
     7201 aaatatggga tgcaatatca cctaacg
//
LOCUS       DAOQDO010000028         7198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_5968_length_7198_cov_2.992860, whole genome shotgun sequence.
ACCESSION   DAOQDO010000028 DAOQDO010000000
VERSION     DAOQDO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5968_length_7198_cov_2.992860"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..778)
                     /gene="icmH"
                     /locus_tag="PKZ53_00930"
     CDS             complement(65..778)
                     /gene="icmH"
                     /locus_tag="PKZ53_00930"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HNJ39025.1"
                     /translation="MPALASEASFTTQTPLTPEVDLVTLASPMFELVMRIRAGLVQPS
                     MELQTTIDRMVTRLEQRGRGLRYAEKQLEAARFALASYIDEMVLMADFPFHDEWEKYP
                     LQLKYFGEHLAGVRFFERLTELLKDVERQGDLVELYYMCLLLGYKGRFRDYFEDELVD
                     VIRQVEQALRQVNRLRTVAQSPHWLLDDQPGLSEETRWPKWLLGLGTGSLGVGILAFL
                     VFKLSIERVLQAALTSLAW"
     gene            complement(865..1329)
                     /locus_tag="PKZ53_00935"
     CDS             complement(865..1329)
                     /locus_tag="PKZ53_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39026.1"
                     /translation="MGCLTEKIRYGKLFAQGVPSRPVTGVNVTNVEVRQKVVLDPVFQ
                     SQLYQGEPETRWIGFGVGYGERNIPADVFFVQHLLNFLITHGGLVPLALIAETGQIDG
                     ATTFAIRYFQRSHLHHPNPDGRVTPNSQTLQKMVEVVGLIRHRLEATKSGAD"
     gene            1723..4152
                     /locus_tag="PKZ53_00940"
     CDS             1723..4152
                     /locus_tag="PKZ53_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ39027.1"
                     /translation="MEPTAARALPIILDLHNGTLDLALMTEPVSRLWMAEDDYSQGSL
                     TFHLALGHFPPPQAVDFYVMAQALMNHREAHRVPNLLKLCREDLGLWRAALEMEAGYR
                     GGAIALDAIEELTTAADPFFQSLHLLQEAMYLRRTNTGDRSEIKRRLHQALTVFQNSS
                     VFETGRTLLALSQVEASYDLALKTTEQALECFSKCKNRYYESDARLAMESIREKHRER
                     RSTSRSRTVDLGFLIRPLIQATDLEAFFSGLIQVLTSRTFLALDHVTLLHKTAGGEHI
                     VAGHHAGPLEKETLSNTFDLGCGYRLVVSQATDPVALEMILPLAKRELIRLARAFPIT
                     HSVYRRQFGRFTYYDSTMDEILNLLEIAGKDQPEAAVMLRGEPGVGKTDLAYALHLAG
                     PTRSGKFVVYDAATAVDKSFMVAELFGTVKGAFTDAQPRIGKIEEAGNGTLFIDEIGE
                     LTPQCQALLLRVLQNRQYQRMGTNEYRPVTCRIILATNQPIEEWCKQSVRSDAQPLFR
                     SDLPSRFRYWIHIKPLHEIPDQIVPTALSFLREQGEFSLSPEAEIFLRSMRWIGNYRS
                     LQNTVGLSVNLAKSFGVSVITPEIIHRAIGHHPLLFAPVTSSVEPPQTALALAASATV
                     TTLPPDQMMSNTGFPFAPEYRTTVGGASGAKMPPSGALPAQSLPYRSAPSAQPLVQPG
                     GQPVAYGYLPPAANPVSPVEPARVHPSNGATAPIQTGSDEAIKQQFIRLFKEVFDEFD
                     YNRRKYFEFSILSNISQIIHQERFPRAKTFSVIYNGLMSSPYVQDTIRRQKIEELGQL
                     FRMINPKWPSE"
     gene            complement(4363..6027)
                     /locus_tag="PKZ53_00945"
     CDS             complement(4363..6027)
                     /locus_tag="PKZ53_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ39028.1"
                     /translation="MKRTLPTPECAALGDLLIAFSVDELDPERKAQVQMHLDLCARCR
                     EELAEFKQAAQLAESLQLTSPVVDRYPEFLRRLAASESKPSSQALLPLTELSALDAEP
                     GSIPLTEGGFAAVVPIFGRRLAVRTGFQRGFDLTLQGRGGNQLLRISAASLTRVATVA
                     AGFSAVAALAVVVMILTISPGLWRTPSPPPPGGGGILGGIEPDSVIPRVKAVGIRPPL
                     VSSSTETLTLMAWNANGKVQAQFVTNNQLTGQPIDLRLQPEKQTKSAQGFENTPAIAT
                     DGTGFLVISELDMGICAWRLNPGTTGSDVYPITLTQRGVQPAVVWIGDRYLAAWTVPD
                     ISSPKIEMVELGSDGRLLTKSPKEVALSLNGNKLRNPVLAGDKNQMALAYFTQDQRVH
                     VQLWQLNAAGPTPPQHIVLPTSDQAEPVNLKIVSRSNEFDLFWIEHGKPDGSEIKFAR
                     LTRTGELSGVKTLVVSTINIPEFDLRETTTDFKLVWLETSTKSAVVLMQHIDLTGERI
                     SHPLNLPTENYRALSACFADPDGTSLVWFAHRPGEAPSFFVASNLP"
     gene            complement(6031..6630)
                     /locus_tag="PKZ53_00950"
     CDS             complement(6031..6630)
                     /locus_tag="PKZ53_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ39029.1"
                     /translation="MTDRRGLRSVPTTQAAQAGELEDFELVRQFKYDQDAQAFEQLFR
                     RHQQYVSHLCLSLLRSRAEAEDALQEIFIKVYRGLNTFEPKVTFRGWLYRITVNHCRD
                     LLDQRARRAEDSEEETLHTLVTYPKQEASVIASMMTEAALDRLKPEYRVAFVLQAVEG
                     HSIAEIAQLLGIGFEAAASRLRRAYQQFIDAYQAINPKD"
BASE COUNT         1718 a         1813 c         1861 g         1806 t
ORIGIN      
        1 cattctcaat tcttcattct tcattcttca ttctcaattc ttcattcttc attctgagtt
       61 ggtattacca ggcgagcgac gtcaaggccg cctgcaaaac gcgttcaatg cttaacttga
      121 acaccaaaaa ggccaaaatt cccacgccga gcgaaccggt tcccaggccc agcagccact
      181 ttggccatct ggtttcttca gagagccctg gttgatcatc cagcagccag tgtggagatt
      241 gggcgacagt tcgtaatcgg ttgacttgcc gtaaggcttg ctccacctgt cggataacat
      301 ccaccagttc atcctcgaaa taatcccgaa accgtccttt gtacccgagc aacaggcaca
      361 tatagtacaa ctcaaccagg tcaccctgcc gctcgacatc ttttaaaagc tcagttaacc
      421 gctcaaaaaa gcgtacgcca gccagatgct cgccaaaata ttttaattgc aatggatatt
      481 tttcccattc atcgtgaaag gggaagtcag ccatcaaaac catttcatca atataggaag
      541 ccagggcaaa cctggcggct tccagttgtt tttccgcata gcgtaaaccc cgcccgcgct
      601 gttccaacct cgtcaccatg cgatcaatcg tggtttgtaa ttccatcgag ggctggacaa
      661 gtccggcccg gatgcgcata acgagttcaa acatcgggct ggccagcgtc accagatcga
      721 cttcgggggt gagcggtgtc tgtgtcgtaa acgatgcttc acttgccagc gctggcataa
      781 tgcctttcct ttcaaaagtt tatcaaatgt gggtgtttga ccctgactgg tcaaagacct
      841 cccaccaaat ctggtcggaa tgccctagtc agcgcccgat ttggttgctt caagtcggtg
      901 gcgaatcaat ccaacaactt caaccatttt ctgcagcgtc tgactatttg gagtaacccg
      961 accatcagga tttgggtggt gcagatgact gcgctggaaa tagcgaattg caaacgtcgt
     1021 cgcaccgtca atctggccag tttctgcaat cagggcgagc ggaactaaac ctccatgggt
     1081 aatcaagaaa ttcagtaaat gctggacaaa aaacacgtca gccgggatgt tgcgttcgcc
     1141 atagccaacc ccaaaaccaa tccagcgggt ttcaggttca ccttgataga gttgggattg
     1201 aaatacaggg tccagcacaa ctttttgcct cacttcaaca ttggtgacat tcacgcctgt
     1261 gactggtcgt gatggaaccc cctgtgcaaa cagcttgcca tacctgattt tttctgtcag
     1321 acaccccacc ggcgctcacc tggcgggagt ttgtctggat caggcgaaac ctgtattttt
     1381 catttaaaaa gaatgaaccc ctgataattc acttttaatt gacatttctt ggatcttaat
     1441 ttaattaaat tttattctga ttctcccaaa gatctgacct gagttcattt tttgattttg
     1501 aattgattct tgttttcctg gaaatactga aaataggttg gttaagactt ctgttgctta
     1561 ttttcaggat gtagcttcga cagccctgag ctttcagaat aggtcaaatg attcaatttg
     1621 aaattgaatt gcacgggatt tgcattacaa cttgaagtca atcaatttaa atttttggat
     1681 tcgacaatat tagaaaagcc tgtcgagagt gaggagtaca ccatggaacc gactgctgcc
     1741 cgtgcattac cgatcatatt ggatcttcat aatggaacgt tggatttggc cctgatgacc
     1801 gagccggtga gccggttatg gatggctgaa gatgattact ctcaagggtc attgactttt
     1861 catctggcgc tggggcattt ccctccgcct caggccgttg atttttacgt catggcacag
     1921 gcgttgatga accaccgcga ggcgcaccgg gtaccaaacc tgctgaaatt gtgccgtgag
     1981 gatttaggac tgtggcgggc agcccttgaa atggaggccg ggtatcgagg tggagctatt
     2041 gccctggatg cgattgaaga actgacaacg gctgccgatc cattttttca gtcgttgcat
     2101 ttactccagg aagcgatgta cctgcgccgg accaataccg gagaccgttc ggaaattaaa
     2161 cgccggctcc accaggcatt aactgtattt caaaatagtt cagtctttga aacaggccgc
     2221 acgttacttg cgctctcaca ggtcgaagcc agttatgacc tggccctcaa gacaaccgag
     2281 caggcactgg aatgtttttc aaaatgcaag aatcggtatt atgagtcaga tgcccggctg
     2341 gcgatggaaa gtatccgcga aaaacatcgt gaacggcgca gcacgtcacg cagccggacg
     2401 gtggaccttg ggtttttgat tcgaccgttg attcaggcga cggacctgga agcttttttc
     2461 tcggggttaa tccaggtact cacctcccga acctttctgg cgcttgatca tgtcacactt
     2521 ttgcataaga ccgctggtgg cgagcatatt gtggctggtc accatgccgg gccgctggaa
     2581 aaagagactc tttcaaacac ctttgatctg ggatgtggat accggctggt tgtatcacag
     2641 gccaccgacc cggtggcact ggagatgatt ttaccgcttg ccaaacgcga gttgattcgg
     2701 ctggcacggg cttttccaat tacacacagt gtctatcgtc ggcagtttgg acgctttacc
     2761 tactatgact ccaccatgga tgagatcctg aatttgctgg aaatcgccgg gaaagaccag
     2821 cctgaagccg ctgtcatgct gcgcggtgaa cctggagttg ggaagacaga tttagcctat
     2881 gcgctccatc tggctggccc cacccggagt ggaaaatttg tggtttacga tgcggcgact
     2941 gcggttgata aatcttttat ggtggccgag ttattcggta cggtcaaagg tgcttttacc
     3001 gacgcccaac cgcgcattgg gaaaattgag gaagctggaa atggaacgct ctttattgat
     3061 gaaattgggg agttgacgcc tcagtgtcag gccttgttgc tgcgtgtttt gcaaaatcgt
     3121 cagtaccagc gaatgggcac caatgagtat cggcccgtca cctgccggat tattctggca
     3181 accaaccagc caattgaaga atggtgcaag caaagcgtgc gctcagatgc gcagccgtta
     3241 tttcgctctg atttgccatc ccgattccgg tactggattc acatcaagcc gctgcatgaa
     3301 attccggacc agattgtccc gacggcgctt tcgtttctgc gcgagcaagg tgagttttca
     3361 ttgtcacccg aagccgaaat cttccttcga tccatgcgct ggattgggaa ttaccgcagc
     3421 ttacaaaata cagtcggcct gtcagtcaat ctggcgaaat catttggtgt gtcggtgatt
     3481 acgccagaga tcatccaccg ggcgattggt catcacccgc ttttatttgc accggtgacc
     3541 agttcggtcg agccgcctca gactgccctg gctctggctg cttcggcgac ggtgacaact
     3601 ctgccgccag atcagatgat gtcaaatacg ggctttccct ttgcacccga gtatcgaacc
     3661 acggttggcg gggcgagcgg cgccaaaatg ccgccgtctg gtgccctacc tgcccaatca
     3721 ttgccctatc gttcagcgcc gagtgcgcaa ccgctggttc agcctggcgg tcaacccgtt
     3781 gcctacgggt accttcctcc cgctgccaac ccggtgtcac cagttgaacc tgctcgggta
     3841 catccttcaa atggcgccac tgccccgatc cagactggaa gtgatgaagc gatcaagcag
     3901 cagtttattc gattgtttaa agaagtattt gatgagtttg attataaccg tcgaaaatac
     3961 tttgagtttt caatcctgag taacatttcg cagattatcc atcaggagcg atttccccga
     4021 gccaaaacat tttcagtgat ttataacggg ttgatgagca gcccgtatgt gcaggacacc
     4081 attcgccgtc agaagattga agaattgggg cagttgttcc ggatgatcaa ccctaaatgg
     4141 ccttcagaat gatcatctgg ctcaaaatag caagaaacac catgcggatg gcaataatct
     4201 ccaagacgtg tgtctggtga ggacgctact gtgtccaggc tttgagaatc aacattcagg
     4261 tcaatggaat aaagcactgg tttacagcct ggccgattgg cttcctgaat gttggttcgc
     4321 aaaaccaggt cccaggtagt aaccaatcaa ttgggtcctg ggtcagggca ggtttgacgc
     4381 cacaaagaaa gagggtgcct cgcctgggcg atgggcaaac cagaccagtg atgttccatc
     4441 cgggtcagca aaacaggcgg acagagcgcg atagttttca gtcggaagat tgagggggtg
     4501 gctgattcgc tcaccagtca aatcaatgtg ttgcatcaac accaccgcac ttttggtcga
     4561 ggtctcaagc cagacgagct taaagtccgt cgtcgtttct cgaagatcga attcagggat
     4621 attgatggtg gaaaccacca gcgttttgac ccctgacagt tctccggtgc gtgtaagccg
     4681 ggcaaatttg atttctgaac catctggctt cccatgttcg atccaaaaca ggtcaaactc
     4741 attggaccgg gaaacaattt tcaagttcac tggctcggcc tggtcggagg tcggtagcac
     4801 aatatgttgg ggaggcgttg gtcctgccgc attgagctgc cacaattgca cgtgaacccg
     4861 ttggtcttgg gtaaaatagg ccagtgccat ctggtttttg tcgccagcca gcaccggatt
     4921 ccggagtttg tttccgttga gagaaagcgc cacttctttt ggtgatttgg tcaacagtcg
     4981 cccatctgat ccaagttcaa ccatttcaat tttaggacta ctgatgtctg gaacggtcca
     5041 ggcggccaga tagcggtctc caatccacac cacggctggc tgaacgccgc gctgggtgag
     5101 ggtaatcgga tacacatcgc tgccagttgt tcccggattg agccgccagg cgcagattcc
     5161 catgtccagt tcacttatca ccagaaatcc ggtgccgtca gttgctatcg ctggggtatt
     5221 ttcaaacccc tgtgctgatt tggtttgttt ttcaggttgg agtcttaaat caatcggttg
     5281 tcctgtcagt tggttgttgg ttacaaactg ggcctggact ttgccgttag cattccaggc
     5341 catcaacgtc aaagtctcgg ttgaacttga gaccagcggt ggtcgaatcc caaccgcctt
     5401 aaccctgggg atcactgaat caggttctat gcctcccagg ataccacctc cgccgggcgg
     5461 aggcgggctg ggtgttcgcc acagacctgg agaaatcgtc agaatcatga ccacaaccgc
     5521 cagggccgcc acggcactaa atccggcagc caccgtcgcg actcgggtca gagacgcggc
     5581 tgagatccgt aacaattgat ttccgccacg accctgaagt gtcaaatcaa acccgcgttg
     5641 aaatccggtt cgcacggcca gacggcggcc aaaaatgggc accaccgcgg caaatccgcc
     5701 ctcagtgaga gggattgatc ctggttctgc atccagagcc gagagttcag tcagtggaag
     5761 caaggcctgt gacgaaggtt tggattcaga cgcggccagg cggcgcaaaa attccggata
     5821 ccgatctaca accgggctgg taagctggag tgattccgcc agttgggcag cctgcttaaa
     5881 ttcggccagt tcctcgcggc accgggcaca aagatcaagg tgcatctgga cttgcgcctt
     5941 gcgttccggg tcaagttcat caaccgagaa tgcaatcagc aggtcgccaa gcgcagcaca
     6001 ttcaggggtg ggtagagttc gtttcataag tcaatctttg gggttaatcg cctgatatgc
     6061 atcaataaac tgctggtaag cccgacgcaa tcggctggca gcggcttcaa atccaattcc
     6121 aagcaattgg gcaatttcgg caatcgagtg gccttcaact gcctgcaaca caaacgccac
     6181 tcgatattcg ggtttgagcc ggtcgagtgc ggcttcggtc atcatggagg caatcacgct
     6241 ggcttcctgt ttagggtaag tcacgagcgt atgcagggtt tcctcttcgg aatcttcggc
     6301 ccgacgggcg cgttgatcca gtaaatcccg acagtggttg accgtgatgc ggtacagcca
     6361 gccacgaaac gtcactttgg gttcaaaggt gttgagcccg cgatagactt taataaatat
     6421 ttcctggagt gcatcttcgg cttccgcccg cgagcgaagc aacgacaggc acaggtgaga
     6481 aacatattgt tgatgacgcc ggaaaagttg ctcaaatgcc tgagcgtcct ggtcatattt
     6541 gaactgccga accagttcaa aatcttccag ttctccagct tgagccgcct gggttgtcgg
     6601 cacgctgcga agtccgcgtc ggtcagtcat gggcgcatgt ccgtttaatg tgaagcgttt
     6661 agaataaggg gcaggaacat cagggcgatc agaaaaagaa gggctgagta tgacgatctg
     6721 gatcagacga gttccttcaa cagtgccata gattgacggc tttttgcctg cgattttttt
     6781 gaagaatgcc gcaaaaaaca gtgactcagc ccacgatccc ttctctgatc aatcaacggt
     6841 tgatcagtga aaaattggtc agagttgata aggtgacagg gtgagaagat gacagagtga
     6901 cagaatgaca aagtgacagg ctgacactta agtaccttgc gataaatttt ctgagatatt
     6961 gggcttggta cctggttgat tgttttccgt gttttccgtg ttttccgtgg ttaagatgtc
     7021 tggaaatctt cggtaaggta cttattgagg tgggcggagg gccttccagt tgcgttcatc
     7081 tggcgtcgct ggatgttgaa taaaggcgtc aagctctgaa attgccgaat atccgatttc
     7141 aggtacatcg gcactctcac caaacaggaa gaatgcctgc cggagtgccg ccgctgat
//
LOCUS       DAOQDO010000029         7075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6130_length_7075_cov_3.823789, whole genome shotgun sequence.
ACCESSION   DAOQDO010000029 DAOQDO010000000
VERSION     DAOQDO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6130_length_7075_cov_3.823789"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKZ53_00955"
     CDS             complement(<1..680)
                     /locus_tag="PKZ53_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNJ39030.1"
                     /translation="MPERIVIFGQYKTGTTALFYRLKHSLTGQVRTFFESDAYTPKLM
                     DFRYHLLAKIILGQPERVDYASFHSFRKRLVLIRDPRDWLVSGTLFMVQQEAALHRSP
                     EVLAQVLALFRQKEQSPADHSLVSILRVLYRLAQNQSLETMAAWMRQHTQWVLAFEQA
                     ERKHLVVRYEDLVDNRLTALERYLGFALSSDTEVATEHAHVPRTKSYGNWKHWFTDED
                     ISFFKPLL"
     gene            969..1610
                     /locus_tag="PKZ53_00960"
     CDS             969..1610
                     /locus_tag="PKZ53_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNJ39031.1"
                     /translation="MSDETPKAEKPKPTPPAKPAGGAAKPTPTKKEAVQVIFDLTGDP
                     LVETLKAKFPGAIKEAQLIHNQQVIRVALESTYQVLCFLKNEAEPDFNFLMDLTAVHF
                     PDRGFEVIYQLYALDEARRFRVKTDVTEQDSVPSVVELWSTANWLEREVFDLFGIRFA
                     NHPDLRRILLPEGWEGHPLRKDYPLEYHDNQWVADHLNILELPVDADLTGKFE"
     gene            1843..2979
                     /locus_tag="PKZ53_00965"
     CDS             1843..2979
                     /locus_tag="PKZ53_00965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNJ39032.1"
                     /translation="MANRPVSNFNFETTLHPDEMIVSMGPQHPSTHGVLRVILKLDGE
                     TVTDLDCDIGYLHRGAEKIFEHRVYPMIAPYFDRLDYIAAVSNGLVYVETVEKAMAIE
                     VPARAQYLRVILTELNRLSSHLLWLGTHALDIGAFTVLLWAFREREEILKIFENFFGA
                     RLTCHAFRIGGMTYDAYDEFEAQVTAFIEMFPKRLEEYETMLNENRIWLGRTVGVGII
                     SGKDAIDLGLSGPPLRGSGVEYDVRKSHPYECYADLDFDIPIGENGDTFDRYLIRMEE
                     MRQSRRLIQQALKKLPGGPVMAKVPKVIRPPAGELYHSNESPKGELGLFLVADGSTQP
                     VRLRVRPPSFVNLQALKKMAVGSLIADVVAGIGTLDIVLGEIDR"
     gene            3077..4411
                     /gene="nuoH"
                     /locus_tag="PKZ53_00970"
     CDS             3077..4411
                     /gene="nuoH"
                     /locus_tag="PKZ53_00970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNJ39033.1"
                     /translation="MNLLTLSLFVPPDEFIFPLMRLGLSRSLIEYIAWPLVQIAVMLT
                     IVMTVVAYLTLAERKISAWMQVRVGPNRVGPWGILQPLADGLKLILKEDIIPLGADHA
                     MFSLAPVLSMVAALTVLVLLPYGPLFASITDVNIGLLFIFSFSSIGILGLILGGWASN
                     SKYPLLGALRSSAQMISYEVVLATSIIGALMFTRTLSMQGIVEAQKSTGIWLGLFQLP
                     AFVIYLFSAIAETNRAPFDLPEAESELVGGFHTEYSGFRFSIFFIAEYANMVVVSAIA
                     ATVFLGGWYLPLGDTLSAWVEARPTIQVALGVIVFAAKVGAVLYLFFWLRWTFPRYRY
                     DQLMDIGWKWMLPAALANVMLTAVVFVVGQQFGLVQRSGLFLQVSPVGKLYFVGMSLL
                     SMIPILILLNIINRDTRMFNLRAQRTKIPFSTRYAQRAEPKPAPASEAESGA"
     gene            4456..5064
                     /locus_tag="PKZ53_00975"
     CDS             4456..5064
                     /locus_tag="PKZ53_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNJ39034.1"
                     /translation="MLLTGWEAYFFYALAITALMMAFQAISTRSPVHCAMYLISTLVS
                     TAGLFLLLQAEFVAGVQVLVYVGGVMVLFLFVVMLVKQRNEEAQAMFSDQTILATIVA
                     LFLGLTLLGVVIGTQRTAFFKPPKEATVPVSVKSVHAPTGTISNDSQLLGQALYTTSV
                     LPFEIASVLLLVAIVGAVVLARDRKQEDQFEAQTSQSAGGKS"
     gene            5184..5495
                     /gene="nuoK"
                     /locus_tag="PKZ53_00980"
     CDS             5184..5495
                     /gene="nuoK"
                     /locus_tag="PKZ53_00980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNJ39035.1"
                     /translation="MTSFLWLSVMMFSVGVAGALTRRNVIIIFMSIELILNAANINFI
                     AFARYWQTQGSISAINGQILTIFVIVVAAAEAAIGLAMVIAYYRNRETIAVDEIHLLK
                     W"
     gene            5624..>7075
                     /locus_tag="PKZ53_00985"
     CDS             5624..>7075
                     /locus_tag="PKZ53_00985"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNJ39036.1"
                     /translation="MDLVILVPLFPLLGFLINGLLAKRLGFSEKIIGSIACLMVGLSF
                     LCSVVAVFQFNQYAHSPATQHQYREQEHTWLEQFNSTHQVKEPVHAAAKSSGHHEELT
                     ATPYVRRVFQWIPSGAAHLTLGPQAHTRTIEFSIDWAYQLDQLSGLYILFITFVGFFI
                     HLFAIGYMHGEEGFYRFFAYLNLFMFMMLTLVLGANFLVMFVGWEGVGLCSYLLIGYY
                     FTKEEAALASKKAFVVNRIGDLGLMLGMFGVFGVFGTLDYDQVMVLAKALPIEPFGLS
                     ALSLTTGVLTLITLALFLGAVGKSAQIPLFIWLPDAMAGPTPVSALIHAATMVTAGIY
                     LVARCNPLFQRAPTTMLIVAMIGCLTAFVAATIGLTQNDIKKVLAYSTVSQLGFMFLG
                     CGVGAFVAGIFHVFTHAFFKAQLFLGSGSVIHGGSSSGHEQDMRFYGNFRKYMPITYK
                     TMLVSLFAICGIFPFAGFFSKDEILAKTLHTEVF"
BASE COUNT         1561 a         1809 c         1865 g         1840 t
ORIGIN      
        1 tgaagcaatg gtttgaaaaa agaaatatct tcatcagtga accagtgttt ccagttgcca
       61 tagcttttgg ttcgcggaac gtgggcatgt tcggtggcaa cttcagtgtc agaagaaagc
      121 gcaaacccca ggtagcgttc caatgccgtc agccggttat caaccaggtc ttcatatcgc
      181 accaccaggt gtttccgctc ggcctgttca aacgccagga cccactgagt gtgctggcgc
      241 atccatgccg ccatggtttc aagcgactgg ttttgcgcca ggcgataaag cacgcgcaga
      301 attgaaacca gggaatgatc agccggcgac tgttcttttt gccgaaacag cgccagcacc
      361 tgtgccagga cctcaggtga acgatgcagc gcggcctctt gctggaccat aaacagggtg
      421 ccgctcacca gccagtcccg aggatcgcgg atgagcacaa gccgcttgcg gaatgaatga
      481 aaactggcgt aatccacacg ttctggctgc cctaaaatga ttttggccag caggtgatac
      541 ctgaaatcca tcagttttgg agtgtaagca tctgattcaa aaaatgttct gacctgtccg
      601 gtcagggaat gtttcaaccg ataaaatagt gcggtggtac cggttttgta ctgcccaaaa
      661 atcacaattc gttctggcat aagggaaagt atcctttcaa tcgttgtgac ctgtgctgac
      721 gttttagcac gtttcatcag agggataacc tgttgaaagt caacggagtg acaggtcaca
      781 gcactctaaa tgatgcgact tgaatgagga aaggctagcc gtcgccagac ttttcgtgta
      841 gagttccggc tgctcgttgc cccagtgcgt ctccaaaaaa actctttcca ccgccttcat
      901 ctcctttttg ctcaatccag ctcagccggg cacgctcaac cgaactggta cccccttgcg
      961 agtgaactat gtcggatgag accccgaagg ccgagaagcc taaaccaact cccccagcca
     1021 aacccgccgg aggtgccgcc aagccgacac cgacgaagaa agaagccgtc caggttattt
     1081 ttgacctgac cggcgaccca ctggttgaaa ccctcaaagc caaatttccc ggtgccatca
     1141 aagaagccca actgattcac aatcagcagg taattcgggt tgcgctcgaa tcaacctatc
     1201 aggtgctgtg ttttcttaaa aacgaagccg agccagactt taattttttg atggatctga
     1261 ccgccgttca cttccctgac cgtggatttg aagtcattta tcagttgtat gcgctggatg
     1321 aagcccgtcg gttccgggtc aaaaccgatg tcaccgaaca ggacagcgtt ccatccgtgg
     1381 ttgaactctg gagcacggcc aactggctag agcgcgaagt ctttgatttg ttcggtattc
     1441 ggtttgccaa tcacccagac ttaagacgga ttctgcttcc ggaaggctgg gaaggtcacc
     1501 cgttacgcaa agattacccg ctggaatacc acgataatca atgggttgcc gatcatttaa
     1561 atatccttga actcccggtc gatgccgatt tgacggggaa atttgaataa agacatcggg
     1621 ctgaagatgt cgggctgaag atgtcgggct gaagattcgc aagctcaggg ctgaagacac
     1681 cgggctgaag attcgcaagc tcagggctga agaagtcggg taatttagtt ttaagcccga
     1741 tgtcttcagc cccaagccca atgtcttcag ccccaagccc gatttcttca gccccgagtc
     1801 tgaaaaaacc aacctcaccc ctgaggctcc acaccaagac ctatggcgaa ccgtccagtc
     1861 tccaacttta actttgaaac cacacttcat ccagatgaga tgattgtgag tatgggacca
     1921 cagcacccaa gtacccacgg ggtgttgcgt gtgattttga agcttgatgg cgaaacggtc
     1981 acggacctgg attgtgacat tggctatctg caccgtggag cggaaaagat ctttgagcat
     2041 cgggtctatc cgatgattgc tccctatttt gaccggctgg actatatcgc ggcggtctca
     2101 aacgggctgg tatatgtcga aaccgttgaa aaagccatgg cgattgaggt tccggcccgg
     2161 gcccagtatc tgcgggtgat cttgaccgaa ctcaaccggc tgtcgagcca tttactctgg
     2221 cttggcaccc acgcccttga tattggcgcc ttcacggttc tgctctgggc gtttcgtgaa
     2281 cgcgaagaaa ttctcaaaat ttttgaaaat ttctttgggg cacgcctgac ctgccacgcc
     2341 ttccgcattg gtggaatgac ctatgatgcc tatgacgagt ttgaagccca ggtcacggcc
     2401 tttattgaga tgttccccaa acgactggaa gagtatgaaa ccatgctcaa tgaaaaccgc
     2461 atctggcttg ggcgaacggt tggagtcggt atcatttccg ggaaagatgc gattgacctt
     2521 ggactgagcg gaccgccgct tcgtgggtca ggcgtggaat acgatgtccg caaatcacat
     2581 ccatatgagt gctatgccga tctggacttt gacatcccga taggtgaaaa cggggacacg
     2641 tttgatcgct atctgatccg gatggaagaa atgcggcaga gccgacgcct cattcagcag
     2701 gcactgaaaa aactgccggg cggcccagtc atggcaaagg tgcccaaggt gattcggccc
     2761 ccagccggtg aactctatca ttcaaatgaa agccccaaag gcgaactcgg actgtttctg
     2821 gtcgccgatg ggtcaactca accggttcga ctccgggttc ggcctccatc atttgtcaat
     2881 ttgcaggcgc tcaaaaaaat ggcggttggc tcgctcatcg ccgatgtagt tgcggggatt
     2941 ggaacacttg acattgtcct gggtgaaatt gaccgctgac agaatgtcag taccacctgc
     3001 gtcagcgggt gggtccaaga gtcaaagctg tgccttgccc ttatcttgtc actttgtcat
     3061 ctggagagtt tttgaaatga atttgctgac attaagttta tttgttccac cagatgaatt
     3121 tattttcccg ttgatgcggc tgggtttatc gcgcagtttg atcgagtaca ttgcctggcc
     3181 gctggtgcaa attgccgtca tgctcacgat tgtgatgacg gtggtggcct atctgacgct
     3241 ggccgagcgg aaaatcagtg cctggatgca ggttcgcgtt gggccaaacc gggttgggcc
     3301 ctggggaatc ctccaacccc tggccgatgg actcaagctc attttaaaag aagacatcat
     3361 cccgctgggt gccgatcacg ctatgttttc gctggcacca gtactgagca tggtggccgc
     3421 gttgacagtg cttgtcttgc ttccgtacgg cccactcttt gccagtatca ccgatgtcaa
     3481 cattgggctg ctctttatct tctcgttttc atccattgga attttagggc tgatcctggg
     3541 cgggtgggcg tcaaacagca aatacccgtt acttggggcg ctgcgttcat cagcgcaaat
     3601 gattagttat gaagttgttt tggccacctc gatcattggc gccctgatgt ttacccggac
     3661 cctttccatg cagggaattg tcgaagccca gaagtcaacc ggcatctggt tgggactctt
     3721 tcagttaccg gcgtttgtca tttacctctt ctcagccatt gccgaaacca atcgcgcccc
     3781 gtttgatctg cccgaagccg aatcagaact ggttggcgga tttcacaccg aatacagcgg
     3841 ctttcggttt tcaatctttt tcatcgctga atatgccaac atggtggtgg tttcagcgat
     3901 tgcggccacc gtctttttgg gtgggtggta tttgcccctg ggtgacaccc tatcagcctg
     3961 ggttgaggct cgaccaacga tccaggtggc gcttggggtc atcgtctttg ccgccaaagt
     4021 gggagcggtt ctgtatctat tcttctggct gcgctggacg tttccccggt atcgctatga
     4081 ccagctaatg gatatcggct ggaaatggat gctgccagcc gccctggcca atgtgatgtt
     4141 aacggcggtt gtgtttgtgg ttggacagca gtttggattg gttcaacggt caggtctgtt
     4201 cctgcaggtt tcaccagttg gaaagctgta ctttgtcggg atgagcctgc tctcgatgat
     4261 tccaattttg atcttgctga acatcattaa ccgtgacacc cggatgttta atcttcgggc
     4321 ccaacggacc aagattccgt tcagcacccg ctatgcccag cgtgctgaac ccaaaccagc
     4381 accagcttca gaagctgagt ctggtgcttg acggcggaat tccgaacttt tttcactcag
     4441 gtttcaggtc tgtttatgtt gctcaccgga tgggaagcct attttttcta tgcactggcg
     4501 atcacggcac tcatgatggc gtttcaggcc atttcaaccc ggagcccggt ccactgtgcg
     4561 atgtatctga tctcaacact ggtcagcacg gcggggctgt tcctgttgtt acaggctgag
     4621 tttgtcgccg gtgtacaggt gctggtctat gtcggcggtg tgatggtgtt gtttttgttt
     4681 gtcgtcatgc tggtcaaaca gcgcaacgaa gaagctcagg ccatgtttag tgaccagacg
     4741 attctggcca cgattgtagc gctgtttctg ggattgacct tactcggtgt cgtcatcggc
     4801 acccagcgaa cagccttttt taaaccgccg aaagaagcaa ccgtgcccgt ctcagtcaaa
     4861 agcgtgcatg cgccgaccgg caccatttca aacgattcac aactgctcgg ccaggcactc
     4921 tatacgacgt cggtgctccc gtttgaaatc gcttcggtat tgttgctggt ggcaattgtc
     4981 ggtgccgtgg tactggcgcg agatcgcaaa caagaagacc agtttgaagc ccaaactagc
     5041 caatcagccg ggggcaaatc atgaatctgc tcatacattt cggaacacaa tggatgtggg
     5101 gactgctggc actggtgtgg caggaggcaa ccaccccttc gaaatcacca accggctggc
     5161 tggctggcta tcaacaggca gacatcacgt catttctctg gctcagtgtg atgatgtttt
     5221 cagtcggcgt cgccggggcg ctgacccgac gcaacgtcat cattattttt atgtcaattg
     5281 aactgattct gaatgcagcc aatatcaact tcattgcctt tgcccgctac tggcaaaccc
     5341 agggaagtat cagtgccata aatggacaga ttttgaccat ctttgtgatt gtagtggcag
     5401 cagccgaggc ggcgattgga ctggcgatgg tcattgccta ttaccgcaac cgtgaaacca
     5461 tcgcagttga tgaaattcat ttgttgaaat ggtgaaaacc agggctgaag aaggcgggct
     5521 gaagaaaacg ggctgaagaa gatgggctga agaattgttt ttatttcatc ctcatccctt
     5581 caatcgccgc aagccctcag cccttaatcc actggtgaac tctatggatc ttgtgatttt
     5641 ggttccgttg tttccgctcc ttgggttttt gatcaacgga ctgctcgcta aacgattggg
     5701 attttcagag aaaatcatcg gcagtattgc ctgccttatg gtggggctgt cgtttctgtg
     5761 ttcagtggtg gcagttttcc agtttaacca gtacgcccat agcccggcca cccagcacca
     5821 atatcgcgag caggaacata cctggctgga acaattcaac agtacccatc aggtcaaaga
     5881 accggttcac gcagcggcaa aatcatctgg tcaccacgaa gaactcaccg ccacgccata
     5941 tgtgcggcgg gtgtttcaat ggattccgag cggcgctgca cacctgaccc tcggtccgca
     6001 ggcccacacc cgaactatcg aattttccat tgattgggcc taccagttgg accagctttc
     6061 gggcctctat attttgttca ttacctttgt cggctttttt attcacctgt ttgccattgg
     6121 gtatatgcat ggcgaagaag ggttttatcg gttttttgct tacctgaacc tgtttatgtt
     6181 catgatgttg acgctggttc tgggagcaaa cttcctggtg atgtttgtcg gttgggaagg
     6241 tgtcgggctg tgttcctatc tgttgattgg atactacttc accaaggaag aagccgcgct
     6301 ggccagcaaa aaagcctttg tggtcaatcg gatcggggat ctcgggttga tgttgggaat
     6361 gtttggcgtg tttggtgtct ttggcacgct cgactacgac caggtgatgg ttctggccaa
     6421 agcgcttccg attgagccgt ttgggctgag tgccttgagc ctgacaactg gcgttttgac
     6481 cctgattaca ctggcattgt ttttaggcgc ggtggggaaa agcgcccaga ttccattgtt
     6541 tatctggctt ccagatgcca tggccggtcc aacgccagtt tcagccctga ttcacgccgc
     6601 cactatggtg acagccggga tttatctggt cgcccgatgc aatccgctct ttcaacgcgc
     6661 ccccacgacc atgttgattg tggctatgat tgggtgcctc acagcctttg tcgcagccac
     6721 aattggtctt acccaaaacg acatcaagaa agtcctggcg tattcgacgg tttcacagct
     6781 tgggtttatg ttcctggggt gtggtgttgg ggcgtttgtg gctggaattt tccacgtttt
     6841 cacccacgct tttttcaaag cccagctctt tttgggatct ggaagtgtca tccacggcgg
     6901 gtcgtcaagt ggccacgaac aggacatgcg gttttacggg aatttccgca agtacatgcc
     6961 gattacctac aaaaccatgc tggtgtcact gtttgcgatt tgcggcatct tcccgtttgc
     7021 cgggttcttc agcaaagatg aaattctggc gaaaacgctc cacaccgagg tcttt
//
LOCUS       DAOQDO010000030         7061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6149_length_7061_cov_3.826577, whole genome shotgun sequence.
ACCESSION   DAOQDO010000030 DAOQDO010000000
VERSION     DAOQDO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_7061_cov_3.826577"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..439
                     /gene="hpt"
                     /locus_tag="PKZ53_00990"
                     /pseudo
     CDS             35..439
                     /gene="hpt"
                     /locus_tag="PKZ53_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
     gene            708..2876
                     /locus_tag="PKZ53_00995"
     CDS             708..2876
                     /locus_tag="PKZ53_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39037.1"
                     /translation="MAVRNTGSLSNTVESGGGRQAGKTAADLIQVGKEAFAHGEVTEA
                     MTTFESVIARYPSQAAEAWYYLGITLISQGRLDQAVEAYQNAIARSATPYPEAHHNLG
                     LTLAQIGQIEAGLSEIEQALRLQPDFAAAHYSRGTLLKQLHRSSEAKTAFQEAIRSAP
                     QQPWSYLHLATIWYELGNPEMARDVLGQLLTQHPEFLAGHLTLGQMALDQKAYADALA
                     SFQAAVDVNPQAIEGWTGLGQACRLSGDWGRAVASFQAALQLDPHRAQLHHELGMVFY
                     ESGNVAAAVQEFETAVKTDPDLTTAYLDLGIALRDFGKLDEAAEAFREALSIEPKLPR
                     AHHNLAVVLFEQGKYYETRRTNEPLEAIQRAIDYSDQPYPKAYFNLGNFYYAQGNIEK
                     ATEAYRQAIQAQPDFAFAHRNLSNALARQNKLDQAYQHLQLALTHGDTDVAAYHNLGI
                     LLLRQKKYAEAIQAFEKAVEQRTTDAESWRGVGLASLLNGQLPQAHQAFTTAIHRRGG
                     RYAEAQYDLGRVQAAQGNHEAACKSFQLTLTEMGGAYWRAHYQLSHSQAALSQFPLAI
                     QSLRMAIEQFGQPFPEGQYELGMLLVRAGNALDATQVFRTAIDQRKGKYPEAYYRLGR
                     IYSQQGNPTAAITAYRTAIEQRGGSYSEAYQDLGRVLYRTGDLNGANEAYSAAVRFQG
                     KTIVPADTSKSPISQNPATELDEDFLKRLRQAAPIYDTGG"
     gene            3011..3472
                     /locus_tag="PKZ53_01000"
     CDS             3011..3472
                     /locus_tag="PKZ53_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HNJ39038.1"
                     /translation="MAVETLCVLLKGTPRVIVQLLKEKGPLTVEALVKETGYPEKEVR
                     KVIPELEKMKLVTKSDGAAYRYSCLSEMQQMQKAPFNNDLGRKVQQSTCTECWKKWVG
                     QQTVLMNHFGLNPIDPQAQKFLFGAMDSFFFGDGTPPMMVDLSLQGTIKHE"
     gene            3671..4720
                     /gene="mltG"
                     /locus_tag="PKZ53_01005"
     CDS             3671..4720
                     /gene="mltG"
                     /locus_tag="PKZ53_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNJ39039.1"
                     /translation="MTNKSLLARFRPVLIGLGLICALCGGFAYWVYHSLTTPLTHQAA
                     DRIIEIESGMGTRGILVRLWQEGVISSRFPLLVYLTVNRNQRPLQAGEYQFESPITPL
                     KVIEKIQRGQVITHELTIPEGLNIFEVENLLKNRDSATPVNGQAVVTELRNITLIRDL
                     DPTASTLEGYLFPDTYVYARNTTSAQLVTQMVRRFRDVFTPQFQARARELGMSVRQVV
                     TLASLIEKEGKVDEDRALISSVFHNRLKRGMKLDCDPTFMYAAMLDGTWDGNVNNPQH
                     RRSLSPYNTYLAPGLPPGPIASPGAKSIQAALYPAESEYIYFVLNEGGKHRFSRTDAE
                     HAAAVAEYRRLQGRR"
     gene            complement(4742..5713)
                     /locus_tag="PKZ53_01010"
     CDS             complement(4742..5713)
                     /locus_tag="PKZ53_01010"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNJ39040.1"
                     /translation="MKNRLKFLCILFFLVIPLLLTAPDIPLVVSAWQQVATTEAAIET
                     VVHSKPKTTNGSKVDQVVVKHPFPFQVGESLTFEFKFSRFPIYGTIGQLNMNVVPVEN
                     LTSEVKKDDPSPIPASKSSEQETRFGLQALAESKGFIPALFGLKVRDTFTSVVDLTDF
                     SSLYMRKDIDEGKKHKKQVSLFHREKPEIHFSETDLSVANAQAAARTIEGVPWTTDLL
                     SFWYMLRMQPFKEGETIPMVLYDDGKIFNVDVVVGKSEKISVEAGKYTARKLTIKAHE
                     AQFIRRKGEYLIWISEDKNRLPVKVRFRTSSGTLTAELVKKNIPSLK"
     gene            5968..>7061
                     /locus_tag="PKZ53_01015"
     CDS             5968..>7061
                     /locus_tag="PKZ53_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39041.1"
                     /translation="MKPLAVVCGVVWFLLATFSVGAQTPVPAQSWPQYRKENTLGARV
                     DPLPVPPRPPGTFFGINTGGSPASSMVGDLSGTGVADIVMIRNGRISATRNGQLLFDN
                     FYGMTDIATITDLDGDGPAEIIAFGTVTQDLMVIQSDGGIRRSFRFPEFTLLAAPFIK
                     VADLIPTRPGKEIVVFPDHTATEGDAMGYFFDSQLQTYATPVVKNLNGGQLNSPQLAI
                     GDVDSNGVLDVVVVGRPWLLIYNVDGTLRSQTEFREGDPEGRHYGLMTLANVDADPKD
                     LEVVVIGDEIPVLAGNGKLQAITVFKLTPTPTRLWGGTFPNENPRACLNSVVDLNGDG
                     RAEIVLNVWDKTTQNIRIYNGTGDANGNAV"
BASE COUNT         1703 a         1797 c         1851 g         1710 t
ORIGIN      
        1 ccttgcgcgc ggcgattgag caagccaatg cgctgccagc ctgttcaccg ctgatctgat
       61 gcggtgtgtt gatctgcctg tcaccgttga ctttatcgcc gtatcaagct atggggcggc
      121 tgccaaatcc tcaggtgaag tccgaatctt gaaagatctt gacaccagtc tcaaagggcg
      181 tgacgtgctg gtggtggaag acattgtgga tacaggattg accctgaatt atctgcttga
      241 caattttcgc cgacgtgatg cggcatcagt tcaagtcgct gcgcttttgg acaaaccaga
      301 acggcgactc gctcatgttg atgtggcata cactggcttt acgattccca atgagtttgt
      361 ggttggctat ggtcttgatt acgcggagcg gtatcgcaat ctgccatata ttgccgtctt
      421 acaacttgaa gcatcgtaat ggcatagcgc gtgatgactg tttttgtgac agataccttc
      481 tgagccggac ccccattcca gctctcagtc aggtttttgt ccccacatat cccctcggat
      541 ctcacacaac cagcaatcgt ttttcggcgt gctatcaccc aatgatagtc acctgagctg
      601 cttttccagc gatggggtta tcctgtttga gttgggaaag cagagatgcg gaattttcac
      661 aggccaggga aatcccaaac actttattct gacgcaaagg ggggcacatg gctgtgcgca
      721 ataccggtag tttatcaaac acagtcgagt ctggtggtgg gcgtcaagca gggaaaacgg
      781 cggctgacct gatacaggtc gggaaagagg catttgctca tggggaagtt accgaagcca
      841 tgaccacatt tgagtcggtc attgccagat acccctctca ggcagccgaa gcctggtatt
      901 atctgggaat tactctgatc agtcagggac ggctcgacca ggccgttgag gcgtatcaga
      961 atgccatcgc ccgctcggca accccgtatc ccgaagccca tcacaatctt ggtttgaccc
     1021 tggcccaaat cgggcagatc gaagccggac tcagtgaaat tgaacaggca ctgcggctac
     1081 aacctgattt tgccgcggct cattacagtc ggggaacact cctcaaacaa ctccatcgtt
     1141 catccgaagc caaaaccgca tttcaagaag ccattcgctc ggcaccacag caaccctggt
     1201 catacctgca tctggcgacc atctggtatg aattgggaaa tccagagatg gcccgcgacg
     1261 ttttaggtca gctcctgacc caacaccctg aatttttagc tggtcacctg acattgggac
     1321 agatggcgtt ggaccaaaaa gcctatgctg atgccctggc cagttttcag gcggcggttg
     1381 atgtgaaccc acaggcaatt gaaggctgga ctggattggg acaggcttgc cggctgtcag
     1441 gcgattgggg acgggccgtg gccagtttcc aggctgccct ccagcttgat ccacaccggg
     1501 cccaactcca tcacgaactg ggcatggtat tttatgaaag tgggaatgtc gccgccgccg
     1561 tccaggaatt tgaaactgcg gtcaagaccg atcccgatct cacaactgcc tatctggatt
     1621 tggggattgc cctacgtgat tttggcaagc tggatgaagc cgccgaagca tttcgcgaag
     1681 cgctttcgat tgaacccaaa ttgccccgtg cccaccataa tctggccgtt gtgttgtttg
     1741 agcagggaaa atattatgaa acccgtcgga ccaatgaacc acttgaagcg atccagcggg
     1801 cgattgacta ttcagatcag ccatatccaa aagcttattt taatcttgga aacttttatt
     1861 acgcccaggg aaatatcgaa aaagccactg aagcctatcg tcaggcgatt caagcccaac
     1921 cagattttgc ctttgcccac cgcaatctca gcaatgccct ggcccgtcaa aacaaactgg
     1981 atcaagccta tcagcatttg caactggcct tgacgcacgg cgatacggat gtggcggctt
     2041 accacaatct gggcatcttg ctgctgcgcc agaaaaaata tgctgaagcc atccaggctt
     2101 ttgaaaaagc agttgagcaa cgaacgaccg atgctgaaag ctggcgaggt gttgggctgg
     2161 caagtttgtt aaacgggcag ttacctcaag ctcatcaggc gtttacaacc gcaatccatc
     2221 gccgtggtgg acggtatgcc gaagcacagt atgatctggg gcgggttcag gctgcccagg
     2281 gaaatcacga agctgcctgt aaatcatttc agttaacgct cacggaaatg ggtggggcat
     2341 actggcgagc ccactatcaa ctcagtcatt cccaggccgc actgagccag ttcccgctgg
     2401 ccattcagtc gcttcggatg gccattgagc aatttggaca accatttcca gaaggccagt
     2461 atgagcttgg gatgttgctg gttcgtgcgg ggaatgccct ggatgcaacc caggtctttc
     2521 gaacggccat tgatcagcgc aagggcaaat atccggaagc ctattaccgg ctgggacgga
     2581 tttattcgca gcaaggcaac ccaactgccg ctattaccgc gtatcgcacc gcgattgaac
     2641 agcgtggagg gagctattcc gaagcctatc aggatttagg gcgggtgctg taccggaccg
     2701 gagacctcaa tggtgccaat gaagcctaca gtgctgccgt tcggttccag ggaaaaacga
     2761 ttgtgccagc cgacacgtca aagtctccga tcagtcaaaa cccagccacc gaactggatg
     2821 aagatttcct gaaacggctg cgtcaggcgg ccccaattta tgacactggt ggatgagtca
     2881 gtgctggcaa cgaccccatc gcccgccaaa gactgcttgc atcatctttg caacccttgc
     2941 tattgaaagg ggtgcagaaa atccttcctg cgtgcgaaac cagtttgaat ttccatttgg
     3001 agtacaactt atggcagtcg agacattgtg tgtgcttttg aaaggcactc cccgcgtaat
     3061 tgttcaatta ctcaaagaaa aaggcccgct cacggttgag gcgctggtga aagaaaccgg
     3121 ctatccggaa aaagaagttc gcaaagtcat tccggaactc gaaaaaatga aattagtcac
     3181 caaatctgac ggggcagcct atcggtattc gtgcctgagt gaaatgcagc aaatgcaaaa
     3241 agcaccattc aacaacgact taggtcgcaa ggttcaacag tcaacctgta ccgaatgctg
     3301 gaagaagtgg gttggtcagc aaacggtcct gatgaatcat ttcgggctga atccgattga
     3361 tccacaggct caaaagtttt tgtttggtgc catggattca tttttctttg gcgatggaac
     3421 cccgcccatg atggttgatc tttcgttgca aggaaccatc aaacacgaat aagaactgag
     3481 aaaaccaggg ctcagggctg aagaaagtgg gctcagggct tggggctgaa ggctcgcaag
     3541 ctcggggcaa tcgaaggggt gagggatgaa ataaaaccaa cttttcaacc cgacttcttc
     3601 aacccgaaat cttcagcccg aagtcttcag cccgaagttt tcagccctgg ttctctaggt
     3661 gcattcactc atgaccaata aatcacttct tgcccgcttc aggccggtcc tgataggact
     3721 tggcctcatc tgtgccctct gtggaggctt tgcttactgg gtatatcact cactgaccac
     3781 ccccctgacg catcaagccg ctgaccgcat cattgaaatt gaatctggaa tgggcacccg
     3841 gggaattctg gttcgactct ggcaagaagg ggttatttca agtcggtttc ccttgctggt
     3901 ctatctcacc gtcaatcgga accagcgacc actccaggct ggcgaatatc agtttgaatc
     3961 acccatcacg ccactcaagg tgatcgagaa gatccagcgc gggcaggtga tcacccacga
     4021 actcaccatt cctgaaggtt tgaacatctt tgaagtcgaa aatctgctca aaaatcggga
     4081 ttctgcaact ccggtcaatg gtcaggccgt ggtgaccgaa ctccgcaata ttacgttgat
     4141 ccgggatctc gacccaaccg cttcaaccct ggaaggatat ttattccccg atacatacgt
     4201 gtatgcgaga aacaccacca gcgctcagct tgtgacccag atggtgcgcc ggtttcgcga
     4261 tgtgtttacg cctcagtttc aagctcgcgc ccgagagctt gggatgtcag tccgtcaggt
     4321 tgtgacgctg gcctcgctga ttgagaaaga aggaaaagtg gatgaggatc gggcgttgat
     4381 ttcaagtgtg tttcataacc ggctcaaacg cggcatgaaa ctggattgtg atccgacctt
     4441 tatgtatgcc gcgatgctgg atggaacctg ggatgggaat gtcaataacc cccagcaccg
     4501 gcgttcgctc tcaccctata acacctatct ggcaccagga ctgccacctg gaccaattgc
     4561 ctcgcccggt gcgaaatcaa tccaggccgc actctatcca gccgaaagcg aatatattta
     4621 cttcgttttg aatgaaggtg gaaaacatcg gttttcccgt actgacgccg aacatgcggc
     4681 agccgtggct gaataccgac gtttgcaggg caggcggtga taattgacag aggtcaccct
     4741 gtcacttcag gctgggaatg ttctttttga ccagttcggc ggttaatgtg ccgctgctgg
     4801 tgcggaaccg gactttgact ggcaggcggt ttttatcttc tgaaatccag atcaggtact
     4861 cgcctttgcg gcgaatgaat tgggcttcgt gggctttaat ggttagtttc cgggccgtgt
     4921 atttcccagc ttcgaccgag attttttcac tttttccaac cacaacatcc acattgaaaa
     4981 ttttgccgtc gtcatagagc accatcggaa tcgtctcgcc ttctttgaag ggctgcattc
     5041 gcaacatata ccagaaggac agcagatccg tggtccaggg aacgccttca atcgttcggg
     5101 cggcggcctg ggcattggcc accgacagat ctgtttcaga gaagtgaatt tctggttttt
     5161 cacggtgaaa cagagaaacc tgttttttgt gtttcttgcc ttcgtcaatg tccttgcgca
     5221 tgtaaagact tgaaaaatca gttagatcca caaccgaagt aaatgtatcg cgcactttga
     5281 gaccaaacag agccggaata aagcctttgg attccgccag ggcctgcaat ccaaaccggg
     5341 tttcttgttc actggatttg ctggctggga tcgggctggg atcatctttt ttgacttcgc
     5401 tggtgaggtt ttcaaccggc accacattca tgttgagctg gccaatcgtg ccatagatgg
     5461 gaaaccggga aaacttaaac tcaaacgtca ggctttcgcc aacctgaaac ggaaatgggt
     5521 gtttgaccac cacctggtca actttggacc cgttggttgt ttttggtttt gagtggacga
     5581 cggtttcaat tgccgcttcg gtcgtggcaa cttgctgcca ggctgagacg acaagtggaa
     5641 tatctggtgc cgtcaacaac agtggtatga ccaggaaaaa aagaatgcac aaaaacttca
     5701 gccgattttt cataataaga agtcaattgt ttggaataat gtgttttgaa aacgtgtgtg
     5761 tcagcaggcg tctgatgatg cccgaattcg agaggttgcg acaagcgctg cccggaaact
     5821 gatttgccgg ctatgattgg attgactcct ggctgataca acgtgttgct gcaagtctta
     5881 gtcataggct tattcaacat cctgcaaata ctcttaaacc ctaaagctca aagcggcgtc
     5941 aagttgctct actccaaaaa aatgcttatg aaaccattgg ctgttgtgtg tggtgtggtg
     6001 tggttccttc tggccacgtt ttcggtgggg gcgcaaaccc cggtgccagc gcaaagctgg
     6061 ccacagtatc gcaaggaaaa cacgctgggg gcacgcgtgg atccgttgcc agttccccca
     6121 cgcccaccag gaacgttttt tgggatcaat acgggtgggt caccagcttc ctcaatggtt
     6181 ggcgatctgt caggaaccgg cgtggccgat attgtgatga tccgcaatgg ccggatttct
     6241 gccacccgaa atggtcagct cctgtttgat aacttttatg gaatgaccga cattgccacg
     6301 attactgatc ttgatggcga tggcccggca gaaatcattg cctttggaac ggtgacccag
     6361 gatctgatgg taattcaatc agatggtggt atccggcgta gttttcggtt tccggaattt
     6421 accttgctgg cggcaccatt tatcaaagtt gccgatctga tcccaacgcg acctgggaag
     6481 gaaatcgtcg ttttcccaga ccacaccgcc actgaaggcg atgcaatggg gtatttcttt
     6541 gattctcagc tccagaccta tgccacgcca gtcgtcaaaa atctcaatgg cgggcaactg
     6601 aattcgccgc aactggcaat tggggatgtt gattccaatg gggtgctgga tgtggtggtg
     6661 gttggccgtc cgtggctact catttacaac gtggatggaa cgctccgcag ccagactgaa
     6721 ttccgcgaag gtgaccccga aggccgccac tatggattga tgaccctggc caacgttgat
     6781 gctgacccca aagacctgga agttgtcgtt attggcgacg aaatcccggt tttggccggg
     6841 aatgggaaat tgcaggcgat tacggttttt aagttaaccc caacgccgac ccgtctctgg
     6901 ggtgggactt ttcccaatga aaacccacgt gcctgtctca acagtgtggt tgatctcaac
     6961 ggtgatggcc gcgccgaaat cgtgctcaat gtctgggaca aaaccactca aaacattcgg
     7021 atttataatg gcactgggga tgcaaatggg aacgcggtgt t
//
LOCUS       DAOQDO010000031         6979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6262_length_6979_cov_3.466638, whole genome shotgun sequence.
ACCESSION   DAOQDO010000031 DAOQDO010000000
VERSION     DAOQDO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6262_length_6979_cov_3.466638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..3326
                     /locus_tag="PKZ53_01020"
     CDS             276..3326
                     /locus_tag="PKZ53_01020"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ39042.1"
                     /translation="MRLRVFCWFAILMLVTGGCSPSILASQRSKVRASHSKKAPSRQK
                     KLFVNKGKKSIPVNVPRQPPIDREHGFENVKARDQWLMFERMYPFDSIPVDARRLAWE
                     NRPQARFQPNQTQFIWRSLGPTPTNSGFPDNWGQTSGRINTIAVSPTDPNLVLVGAAT
                     GGIWRSVDGGQTFTTTTDDQVDLAVGSIAFAKSNPSVVYAGMGDANGNYLGNGVLKST
                     NAGQTWTRVSNSSLPAPGRILKVGVDPTNPDRVYAAQFDQLIASGSVASSGFYVSSDG
                     GVTWKRTLTGLGRDLAISQGNPEIVYAAMSRVDESGKPAGLYRSTDRGETWTVAYTSP
                     FTQGTLDLQIGVTPANSQVLYIFTGGFSNNAFSLRVEVSTDGGQSWTARNTSNVDTAQ
                     FSYNAYIAVDPNNPDIVYLGTRDIFKSTNGGRAWTNLNNNFQFSGDYTPDRSSAHPDQ
                     HAFAFIPNSPNTFLSGCDGGLYRSSDGGQTFQSSNASLSLTQFYGIAVNPTDPDLIFG
                     GTQDNGTQRRIGSSSTWDEFISGDGGQVVVNPLNPEQLFTTYVYGTIFRWRANGNVFE
                     DVVGSDEVFGEGSQTRIAFIAPFAGNGVDSTLYFGTWRLFVSKDLGDTWKAPAGNLDL
                     TKGIRSGFSPDVLSIIAASSANPNVIYTGSSQGRVMVTADGGRTFKDITAGLPDRFIR
                     SIAIDRQNPATAYLTVSGFRTGHVFKTTNMGTTWADISGNLPDIPALAVLVDRQDPTT
                     LYLGTDIGVFRSTTGGNQWEVFNNGMPPVRVTGFAVHPNGTIRVATYGRGMFELEQRD
                     TSDTTSPTVTVTAPQGGETLSINGQFEITWQATDDTGIRSQEIALSTDAGVTFPTVIV
                     AGLTGATQSFRWTVPETPTQQARIQVSASDLAGNIGRATSAGNFTIQTPPADTVAPTV
                     TVLAPDGPADKLRAGTTFLIRWQSTDNVGVTAHDVLFSTDGGATYPTTLATGLTGAVQ
                     SFNFAIPASQPKTKTARIRVIASDAAGNTGQDDSNANLKIKLAK"
     gene            3522..6575
                     /locus_tag="PKZ53_01025"
     CDS             3522..6575
                     /locus_tag="PKZ53_01025"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ39043.1"
                     /translation="MRLRVFCWFAILMLVTGGCSPSILASQRSKVRASHSKKAPSRQK
                     KLFVNKGKKSIPVNVPRQPPIDREHGFENVKARDQWLMFERMYPFDSIPVDARRLAWE
                     NRPQARFQPNQTQFIWRSLGPTPTNSGFPDNWGQTSGRINTIAVSPTDPNLVLVGAAT
                     GGIWRSVDGGQTFTTTTDDQVDLAVGSIAFAKSNPSVVYAGMGDANGNYLGNGVLKST
                     NAGQTWTRVSNSSLPAPGRILKVGVDPTNPDRVYAAQFDQLIASGSVASSGFYVSSDG
                     GVTWKRTLTGLGRDLAISQGNPEIVYAAMSRVDESGKPAGLYRSTDRGETWTVAYTSP
                     FTQGTLDLQIGVTPANSQVLYIFTGGFSNNAFSLRVEVSTDGGQSWTARNTSNVDTAQ
                     FSYNAYIAVDPNNPDIVYLGTRDIFKSTNGGRAWTNLNNNFQFSGDYTPDRSSAHPDQ
                     HAFAFIPNSPNTFLSGCDGGLYRSSDGGQTFQSSNASLSLTQFYGIAVNPTDPDLIFG
                     GTQDNGTQRRIGSSSTWDEFISGDGGQVVVNPLNPEQLFTTYVYGTIFRWRANGNVFE
                     DVVGSDEVFGEGSQTRIAFIAPFAGNGVDSTLYFGTWRLFVSKDLGDTWKAPAGNLDL
                     TKGIRSGFSPDVLSIIAASSANPNVIYTGSSQGRVMVTADGGRTFKDITAGLPDRFIR
                     SIAIDRQNPATAYLTVSGFRTGHVFKTTNMGTTWADISGNLPDIPALAVLVDRQDPTT
                     LYLGTDIGVFRSTTGGNQWEVFNNGMPPVRVTGFAVHPNGTIRVATYGRGMFELEQRD
                     TSDTTSPTVTVTAPQGGETLSINGQFEITWQATDDTGIRSQEIALSTDAGVTFPTVIV
                     AGLTGATQSFRWTVPETPTQQARIQVSASDLAGNIGRATSAGNFTIQTPPADTVAPTV
                     TVLAPDGPADKLRAGTTFLIRWQSTDNVGVTAHDVLFSTDGGATYPTTLATGLTGTAQ
                     SFNFAIPASQPKTKTARIRVIARDAAGNTGQDESNANLKIKPSKN"
BASE COUNT         1633 a         1850 c         1836 g         1660 t
ORIGIN      
        1 gaccaagatt caaaaacccc gaaccctgaa ccctgaaccc tgaaccagat tttgtgtaat
       61 gattttggat tttcagcact acatcggtgc tggttactct ttctcaacag tgagcgagtt
      121 agctgacacc gattttgctg attgtctgag aaagcagtaa ccagggattt tcgtttcctt
      181 cgcaagtgcc tttcataatt ccccgtctct catccataat cggaaaatct caaatcccaa
      241 ccttttctga tttcaactgg atggagtaat tcccgatgcg tcttcgtgtt ttctgctggt
      301 ttgccatttt gatgctggtg actggaggct gctcgccgtc aatcctcgcg tctcaacggt
      361 cgaaagtccg tgcttcacat tccaaaaaag caccttcccg ccaaaaaaag ctcttcgtaa
      421 acaagggtaa aaaatcaatt ccggtgaatg tgccgcgcca gccaccaatt gatcgcgagc
      481 atggctttga aaatgtcaaa gcccgtgacc agtggttgat gtttgagcga atgtatccat
      541 ttgattcaat tccagttgat gcccgtcggc tggcctggga aaaccgtccg caagcacggt
      601 ttcagcccaa tcagacacaa tttatctggc ggtcacttgg gccgacgcct acaaattccg
      661 gttttccaga taactggggg cagaccagcg gtcgcatcaa cacgattgcc gtttcaccga
      721 ccgatccgaa tctggttctg gttggtgcgg cgacgggtgg aatctggcgc tcggttgatg
      781 gtggacaaac ctttaccacc accacggatg atcaggttga tctggcggtc ggttcaattg
      841 cctttgccaa aagcaatcca tcggtggttt atgctggaat gggcgatgcc aacggaaact
      901 acctgggaaa tggggtgttg aaatcaacca atgcaggtca aacctggacc cgagtcagca
      961 attcatcttt gccggctccg ggccggattt tgaaagttgg cgtggatccg accaatccag
     1021 atcgtgtgta tgcagctcaa tttgatcaac ttatcgcttc gggcagtgtt gccagcagcg
     1081 gattttatgt gtcctcagat ggaggtgtta cctggaaacg gacactgacg ggacttggac
     1141 gtgatctggc catttctcag ggaaatccgg agattgtcta tgccgcaatg agccgggttg
     1201 atgaaagcgg aaaaccggcg gggctgtatc gctcaactga tcgtggcgag acctggaccg
     1261 tggcctacac ttcaccattt acccagggca cgcttgatct gcaaatcggg gtcacgccag
     1321 ccaattcgca ggtgctctat atctttaccg gcggattcag caacaatgcg tttagcctgc
     1381 gggtcgaagt cagtaccgat ggcggccagt cctggacggc acgcaatacc agcaacgttg
     1441 atacggcaca gtttagctac aacgcctata tcgcggttga cccaaacaac ccggacattg
     1501 tgtacctggg aacacgagat attttcaaat ctaccaatgg tggtcgtgcc tggaccaatc
     1561 tcaataacaa tttccaattt tcaggcgact acacgccaga caggtccagt gctcaccctg
     1621 accagcatgc gtttgccttt attccaaata gtccaaacac atttctctct ggatgtgatg
     1681 gtggattata ccgatcttcc gatggtggcc agactttcca atcgtcaaat gcgtcgctgt
     1741 cattaactca attttatgga attgcagtca acccgaccga ccctgatttg atctttgggg
     1801 gaacgcagga taacggtacc cagcgccgca tcggaagttc aagcacctgg gacgagttta
     1861 tcagtggtga tggcggccag gtcgtggtca atccgctcaa tcccgaacag cttttcacga
     1921 cctatgtcta tggcacgatt tttcgctggc gggccaatgg aaatgttttt gaagatgtgg
     1981 ttggttctga cgaagtgttt ggcgaaggaa gccagacccg aattgccttt atcgccccat
     2041 ttgcgggcaa tggtgtggat tcaaccctct attttggcac ctggcggttg tttgtgagca
     2101 aggatttggg tgacacgtgg aaggcaccgg ctggaaatct ggacttgacg aaaggcatcc
     2161 gatcagggtt ttcccctgat gtgttgtcaa ttattgctgc cagttcggcc aatccaaatg
     2221 tgatttatac tggctcgtcg cagggtcggg tgatggtgac ggcggatggc ggacggacgt
     2281 tcaaagacat taccgctgga ttgccagatc gctttatccg aagcattgcc attgatcgtc
     2341 agaacccggc gacggcctat ctgaccgttt ccggttttcg caccgggcac gtgtttaaaa
     2401 ccacgaatat gggcaccacc tgggctgaca tcagcggaaa cctgccggac atcccggcgc
     2461 tggcggtgct cgtggatcgt caggatccaa ccacgctcta ccttggaacc gacattgggg
     2521 tgtttcgatc aaccacaggc gggaatcaat gggaagtgtt caataacggg atgcccccgg
     2581 tgcgggttac cgggtttgcg gttcatccaa atggaaccat tcgcgtggca acctatggtc
     2641 gaggaatgtt tgaattagaa cagcgtgata cctccgacac caccagccca accgtgactg
     2701 tgacggcgcc acagggaggt gaaacactct caatcaatgg ccagtttgaa attacctggc
     2761 aggcaaccga cgacactggc attcgcagtc aggagattgc tctttcaaca gatgccgggg
     2821 tcacctttcc aaccgtaatt gtcgctggac tcaccggagc cacccaaagt ttccggtgga
     2881 ccgttcccga aacacctacc cagcaagccc gcattcaggt gtctgccagt gatctggctg
     2941 gcaatattgg ccgcgccaca agtgccggga attttacgat tcagacaccg cctgctgata
     3001 cggttgcccc aacggtgacc gtgctggcac cagacggccc agctgacaaa ttgcgagccg
     3061 ggacgacatt tctgatccgc tggcaatcaa ctgataacgt tggggttacg gctcacgacg
     3121 tgttgttttc gaccgatggc ggcgccacct atccaaccac gctggccacc gggttgactg
     3181 gagcggtgca gtccttcaat tttgcgattc cagcttcaca accaaaaacc aaaacagcgc
     3241 gaattcgagt cattgccagt gatgccgccg gcaacacggg gcaggacgac agcaacgcca
     3301 acctcaaaat caagctggct aaataatgac aaggtgacag ggtgacaaag tgaccgtgtg
     3361 atacaaattt cttttcatct ggtcattctg ccaccctgtc accttgtcat ttcgtcattc
     3421 ttccttcgca agtgcctttc ataattcccc gtctctcatc cataatcgga agatctcaaa
     3481 tcccaacctt ttctgatttc aactggatgg agtaattccc gatgcgtctt cgtgttttct
     3541 gctggtttgc cattttgatg ctggtgactg gaggctgctc gccgtcaatc ctcgcgtctc
     3601 aacggtcgaa agtccgtgct tcacattcca aaaaagcacc ttcccgccaa aaaaagctct
     3661 tcgtaaacaa gggtaaaaaa tcaattccgg tgaatgtgcc gcgccagcca ccaattgatc
     3721 gcgagcatgg ctttgaaaat gtcaaagccc gtgaccagtg gttgatgttt gagcgaatgt
     3781 atccatttga ttcaattcca gttgatgccc gtcggctggc ctgggaaaac cgtccgcaag
     3841 cacggtttca gcccaatcag acacaattta tctggcggtc acttgggccg acgcctacaa
     3901 attccggttt tccagataac tgggggcaga ccagcggtcg catcaacacg attgccgttt
     3961 caccgaccga tccgaatctg gttctggttg gtgcggcgac gggtggaatc tggcgctcgg
     4021 ttgatggtgg acaaaccttt accaccacca cggatgatca ggttgatctg gcggtcggtt
     4081 caattgcctt tgccaaaagc aatccatcgg tggtttatgc tggaatgggc gatgccaacg
     4141 gaaactacct gggaaatggg gtgttgaaat caaccaatgc aggtcaaacc tggacccgag
     4201 tcagcaattc atctttgccg gctccgggcc ggattttgaa agttggcgtg gatccgacca
     4261 atccagatcg tgtgtatgca gctcaatttg atcaacttat cgcttcgggc agtgttgcca
     4321 gcagcggatt ttatgtgtcc tcagatggag gtgttacctg gaaacggaca ctgacgggac
     4381 ttggacgtga tctggccatt tctcagggaa atccggagat tgtctatgcc gcaatgagcc
     4441 gggttgatga aagcggaaaa ccggcggggc tgtatcgctc aactgatcgt ggcgagacct
     4501 ggaccgtggc ctacacttca ccatttaccc agggcacgct tgatctgcaa atcggggtca
     4561 cgccagccaa ttcgcaggtg ctctatatct ttaccggcgg attcagcaac aatgcgttta
     4621 gcctgcgggt cgaagtcagt accgatggcg gccagtcctg gacggcacgc aataccagca
     4681 acgttgatac ggcacagttt agctacaacg cctatatcgc ggttgaccca aacaacccgg
     4741 acattgtgta cctgggaaca cgagatattt tcaaatctac caatggtggt cgtgcctgga
     4801 ccaatctcaa taacaatttc caattttcag gcgactacac gccagacagg tccagtgctc
     4861 accctgacca gcatgcgttt gcctttattc caaatagtcc aaacacattt ctctctggat
     4921 gtgatggtgg attataccga tcttccgatg gtggccagac tttccaatcg tcaaatgcgt
     4981 cgctgtcatt aactcaattt tatggaattg cagtcaaccc gaccgaccct gatttgatct
     5041 ttgggggaac gcaggataac ggtacccagc gccgcatcgg aagttcaagc acctgggacg
     5101 agtttatcag tggtgatggc ggccaggtcg tggtcaatcc gctcaatccc gaacagcttt
     5161 tcacgaccta tgtctatggc acgatttttc gctggcgggc caatggaaat gtttttgaag
     5221 atgtggttgg ttctgacgaa gtgtttggcg aaggaagcca gacccgaatt gcctttatcg
     5281 ccccatttgc gggcaatggt gtggattcaa ccctctattt tggcacctgg cggttgtttg
     5341 tgagcaagga tttgggtgac acgtggaagg caccggctgg aaatctggac ttgacgaaag
     5401 gcatccgatc agggttttcc cctgatgtgt tgtcaattat tgctgccagt tcggccaatc
     5461 caaatgtgat ttatactggc tcgtcgcagg gtcgggtgat ggtgacggcg gatggcggac
     5521 ggacgttcaa agacattacc gctggattgc cagatcgctt tatccgaagc attgccattg
     5581 atcgtcagaa cccggcgacg gcctatctga ccgtttccgg ttttcgcacc gggcacgtgt
     5641 ttaaaaccac gaatatgggc accacctggg ctgacatcag cggaaacctg ccggacatcc
     5701 cggcgctggc ggtgctcgtg gatcgtcagg atccaaccac gctctacctt ggaaccgaca
     5761 ttggggtgtt tcgatcaacc acaggcggga atcaatggga agtgttcaat aacgggatgc
     5821 ccccggtgcg ggttaccggg tttgcggttc atccaaatgg aaccattcgc gtggcaacct
     5881 atggtcgagg aatgtttgaa ttagaacagc gtgatacctc cgacaccacc agcccaaccg
     5941 tgactgtgac ggcgccacag ggaggtgaaa cactctcaat caatggccag tttgaaatta
     6001 cctggcaggc aaccgacgac actggcattc gcagtcagga gattgctctt tcaacagatg
     6061 ccggggtcac ctttccaacc gtaattgtcg ctggactcac cggagccacc caaagtttcc
     6121 ggtggaccgt tcccgaaaca cctacccagc aagcccgcat tcaggtgtct gccagtgatc
     6181 tggctggcaa tattggccgc gccacaagtg ccgggaattt tacgattcag acaccgcctg
     6241 ctgatacggt tgccccaacg gtgaccgtgc tggcaccaga cggcccagct gacaaattgc
     6301 gagccgggac gacatttctg atccgctggc aatcaactga taacgttggg gttacggctc
     6361 acgacgtgtt gttttcgacc gatggcggcg ccacctatcc aaccacgctg gccaccgggt
     6421 tgactggaac ggctcagtcg ttcaattttg cgattccggc ctcacaacca aagaccaaaa
     6481 cggcacgcat tcgagtcatt gcccgtgatg ccgccggcaa tactggccag gacgagagca
     6541 acgccaatct caagatcaag ccgtcaaaaa actaaccggc ggaaaacatg gggctgaggg
     6601 ttgagggcta ggggttgagg gccatacgcg ccaagctaag gaaatttgag cgcttgtggg
     6661 acaaggggac aaggggacaa ggggacaagg agaaaaaacg aagagtctca attctcgctc
     6721 cccatcggag ggtgttgatg cccacacaaa ctggtttgat gtctccttgt cccggtgtcc
     6781 ccttgtccag atcgagccga cgtctcttat cttggcgctt atggggctaa gggctaaggg
     6841 tgaagaaaac agattcttca gccccaagcc ctataccctc agccccagtc cttcagcccc
     6901 aagccctgag cccggttttt tcagtcctga accagcaaat catccaccac atccagatac
     6961 aggtccgggt ggcggagtt
//
LOCUS       DAOQDO010000032         6891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6386_length_6891_cov_3.974985, whole genome shotgun sequence.
ACCESSION   DAOQDO010000032 DAOQDO010000000
VERSION     DAOQDO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6386_length_6891_cov_3.974985"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKZ53_01030"
     CDS             complement(<1..595)
                     /locus_tag="PKZ53_01030"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ39044.1"
                     /translation="MGFSGSLEKMKITAYVNSDFTSQVGEVFSVYINPEKYSHSYKIC
                     YNDVQAQGSNGGSPEFNRIPSEEVKFELVFDGTGVVPSPIPGVIPFTGDGISAQILAF
                     KNLVFRFNGKIHSPNYLKIVWGKFIFPCRMSALTINYTLFKPDGTPLRARANATFIKF
                     NNERTLALEANQNSPDLAHILTVKAGDTLPLMCYAVYG"
     gene            complement(597..776)
                     /locus_tag="PKZ53_01035"
     CDS             complement(597..776)
                     /locus_tag="PKZ53_01035"
                     /inference="COORDINATES: protein motif:HMM:NF039278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HNJ39045.1"
                     /translation="MSLEISEITIRIGVREDDSGDELRQPKTEIREAELFDREELVED
                     CVRRVLYLLRTQGDR"
     gene            complement(947..1480)
                     /locus_tag="PKZ53_01040"
     CDS             complement(947..1480)
                     /locus_tag="PKZ53_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNJ39046.1"
                     /translation="MTDNYYPPGAFYFSVAVIGSGSILSLLTGIDASFQEVSGIQSEF
                     GFEDVGEGGENRYKHRLPLPAKYSNLVLRRGVVTTDSFLSEWVGQTIGSSLSLPIITQ
                     NLLVTLLNDDGIPLIAWGFVNAYPLKWEFSAMNSQENKILTEKMEFTYNYFERVTLGS
                     PASVAVKLAQLAARMAL"
     gene            complement(1626..2078)
                     /locus_tag="PKZ53_01045"
     CDS             complement(1626..2078)
                     /locus_tag="PKZ53_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNJ39047.1"
                     /translation="MAGEVQNNIWPLPKFYFSVQLGDDNSVSFQEVDGLNSETQVIEY
                     RHGNSPIFYPIKMPGLGRVGNVTMRKGIFVNDARFWTWYNEIKMNTIARRTVVVNLLD
                     ETGAPKMVWTLNNAWVTKLTGTDLKSEGNEVAVESIEIAFETLTVAAP"
     gene            complement(2215..3867)
                     /locus_tag="PKZ53_01050"
     CDS             complement(2215..3867)
                     /locus_tag="PKZ53_01050"
                     /inference="COORDINATES: protein motif:HMM:NF037565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNJ39048.1"
                     /translation="MPATVYRRTPGVYVTELSAFPPSIVGVQTAVPAFIGYTKKAEIS
                     GKPVFMKPIKIGSMVDFVEIFGKEYDPVYQIQEVTDQPKIKNHEYDFQVVEPPTQPTT
                     PPPYPIPNGTTKYFNLVFPTGSTKFNLYNSLRLFYANGGGNCFVVSVGTYEGTTSISK
                     EKLLEGLGVIKEQVGPTMLVVPDAVLLAPTGGKEPGWVSADFQAVTRRMLEQSAELKD
                     RMAILDVYGTPLATKENYEDIINRFREDVGEAGLSYGAGYFPFLATTVIPIDDFDYTN
                     ITGVTGGTPLSEILTWQAANLYNGGYLPGADQGTKRYLAVKADIDAMATTTTEPGITK
                     LNNNLMASLPLLKDMLRIMIAKESILPPSGAMAGIYTYNDATRGVWNAPANLTLSGVD
                     RTTFKLNDEQQGEMNVPVNGKAVDALREFVGRGVVVWGARTLDGNSNDNRYIQVRRTL
                     ITVEQSIKLSLDKFVFAANDANTWVTVVSMISNYLQGFWSQGGLMGSTAQEAFSVQCG
                     LGSTMTPMDVLEGYMIVQVTLQMIRPAEFIELTFKQKMEGLG"
     gene            complement(3903..5339)
                     /locus_tag="PKZ53_01055"
     CDS             complement(3903..5339)
                     /locus_tag="PKZ53_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39049.1"
                     /translation="MRSPLFPNRTTERVLRNPQREKKGVLRDREEPRRYYFQRLMQVT
                     VRHSYYNGEDDLCLDFQVLPTPSTASLMHSIGLVFKAEPGGFSVFYNTREEEDLIQYL
                     GRQTDPGVPGIWTRLSFVFVLTNQFFVNFTNINIKTNPALENFYFTNQRSHRRLEQQI
                     VLHPETFVTERETLEVTPIQVPVVVPPTAKKVKVRAISNEEVLCFPRCFPKELLLKKS
                     PNAITCKDAASGKGEEVCRDLLYINFSVLPEDKYTIEQVAKGGQIIDQQEVLYTIASP
                     VPFCFINLLFTSPTGQYPDHYPVQPLPDGTIGIVPVTYEIKFKARSTYWNYFVIPQRS
                     KERLTNLQIKDVTPDRDPIEFAGPCRVKLANGSIAYRFVSTVPIQLQQHSDFIFQLWG
                     KHGLMVQENILIDRLPVASMQQVLPETKAATCLKLRNSLCPDAADSPNCQTLIAQLCP
                     FDGDGTLTETQLDPVPKIQNFSDIFVAL"
     gene            complement(5401..6699)
                     /locus_tag="PKZ53_01060"
     CDS             complement(5401..6699)
                     /locus_tag="PKZ53_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNJ39050.1"
                     /translation="MSNQDQTVWTCSVDSSTLTASLKVSVNGGSPDPFVMKGVCYSPC
                     PIGGSNSYGPNIGDWFWDAYTGVTNWSGTWSNDLPLIKALGANTIRVYCFIDTQLPPA
                     PPAQIFTHTSFLDACYENGLYVLVGIPLPQSLFVLGQTPQPSADWWTQNLQTTVSEIA
                     NHPAVLGFTIANEVDNGSVDTYGPEANATYWWGQVQAMAQIAKTAAPDKLVGIANHDD
                     PGICQNAASYMATCPSIDFWGVNTYQPQSFSSVFQGYGSLTGAALKPVILTEYGFPST
                     SRTTANTCNPAEIYSDSTTEANVATVLTTMLPAAFAESLNLGLYYFEFCDEWWNQSGY
                     QIAANFTCPAATGPNAPNGGAGGSFNMPNVYTWYGGPPACGFPNYYWDNDGFGLYAVG
                     VGTGRNPADPWDPGTNSPALPLDTRSGRAAVITAIKTSFG"
BASE COUNT         1690 a         1677 c         1799 g         1725 t
ORIGIN      
        1 tgccatacac ggcgtaacac atcaacggca atgtatctcc ggctttgacg gtcaggatgt
       61 gtgccagatc gggcgagttc tggttggctt ccagcgccag cgtgcgctcg ttgttaaatt
      121 tgatgaatgt ggcattcgct cgggcccgca aaggtgtgcc atctggtttg aacaacgtgt
      181 agttaatcgt caaggctgac attcgacagg gaaaaatgaa ctttccccag acaattttca
      241 ggtagttggg gctgtgaatt ttgccgttga accgaaaaac caggtttttg aaggcgagaa
      301 tctgagctga aatgccatct ccggtaaagg gaatcacacc cggaatcggg cttggcacca
      361 cgccggttcc atcaaaaacc agttcgaatt tgacctcttc cgacggaatc cggttgaatt
      421 caggcgaacc gccgttgctt ccctgggcct gaacgtcgtt gtaacagatt ttataggagt
      481 gcgagtattt ttcagggttg atgtacaccg aaaacacctc gcccacctgc gacgtgaagt
      541 cgctgttgac ataggccgtg atcttcattt tttcaagact accggaaaag cccatgttat
      601 cggtctcctt gagttctcaa cagatacaac acacgccgga cacagtcttc gaccagttct
      661 tcccggtcga agagttccgc ttcccggatt tcggttttgg gctgacggag ttcgtcacct
      721 gagtcgtctt cccggacgcc gattcgaatt gtgatttcac tgatttctaa ggacataggt
      781 tagggcttgg ggctgaaaac atcgggcttg gggctgaaaa catcgggctt ggggctgagg
      841 gctgaaaacc ttgggctaag ggctgagggg aaaactccaa attgaattca aatgatttgt
      901 atacccttag ccccaagccc tcagcccgat ttcttcagcc ccagtctcac agggccatcc
      961 gggccgccag ttgcgcaagt ttgacggcca ctgaagccgg gcttcctaaa gtgactcgtt
     1021 caaagtagtt gtaagtgaac tccatctttt cggtcaggat cttgttttcc tgtgagttca
     1081 tggccgaaaa ttcccacttg agtggatagg catttacaaa cccccaggca atcaatggga
     1141 tcccgtcgtc gttgaggagt gtcaccagca gattttgagt gatgatcggc agtgacaggg
     1201 atgatccaat cgtttgccca acccactctg agagaaacga gtcggtggtg acgactccgc
     1261 gccgcaaaac cagatttgaa tatttggctg gcagcggaag ccgatgctta tagcggtttt
     1321 caccaccttc accaacatcc tcaaagccaa attcagactg aatgcccgac acttcctgaa
     1381 aactggcatc aatgccagtc aacagcgaaa ggatcgaacc tgacccaatc acagccacgc
     1441 taaagtaaaa agcaccgggt ggatagtagt tgtctgtcat tgaaacctca aataccaggg
     1501 ctgagggctt ggggcggaag actcgcaagc tcggggcgga agaaaacggg ttcaataccc
     1561 tgagcccaag aatcttcagc ccgaagtttt cagccccgag cctgcgagtc ttccgcccgc
     1621 tgtatttaag gagctgccac cgtcagggtt tcgaaggcga tttcaatgga ttcgaccgcc
     1681 acttcgttgc cttctgattt gaggtccgtc ccggtcagtt tcgtgaccca ggcattgttg
     1741 agcgtccaga ccatctttgg tgcgccggtt tcatcgagca gattgacgac gacggtgcga
     1801 cgggcaatgg tgttcatctt gatttcgttg taccaggtcc agaaccgcgc atcgttgaca
     1861 aagatgcctt tccgcatcgt gacattgccg acccggccca atcctggcat tttgatcggg
     1921 tagaaaatcg gactgttgcc gtgtcggtat tcaatgacct gagtttcgct gttgagtccg
     1981 tcaacttctt gaaaactcac agagttatcg tcaccaagtt gaactgaaaa gtaaaactta
     2041 ggtaacggcc aaatgttatt ttgaacttcg cctgccatga tgcctcctga tgaaagatga
     2101 aaaaaggatt agggaatagc gcagagagaa gaagggaggg catttggaat tcaatgaact
     2161 ccaatggtct caactggttc aatttctttc cctcagccct gagcccaaat tggattagcc
     2221 gagaccttcc atcttctgct tgaaggtcag ttcaataaat tcagcgggtc gaatcatttg
     2281 gagggttacc tggacaatca tatacccttc cagaacatcc atcggtgtca tggtactgcc
     2341 gagtccgcat tgcaccgaga atgcttcctg agccgtcgat cccatcaacc caccctgtga
     2401 ccagaagccc tgcaggtaat ttgaaatcat tgagacgacg gtcacccagg tgttggcatc
     2461 gttggcggca aacacaaact tatcgagcga taacttgatg gattgttcga cggtaatcaa
     2521 tgttcggcgg acctggatat accggttgtc attgctgttg ccatccagag tacgcgcccc
     2581 ccacacgaca acgccgcgcc caacaaactc ccgcagggca tccacggctt tgccattgac
     2641 cggaacgttc atttcgccct gttgctcatc gttgagtttg aacgttgtgc gatcaacacc
     2701 cgaaagcgtg agattcgccg gggcattcca tacaccgcga gtggcgtcat tgtaggtata
     2761 aattccggcc atggctccac ttggaggcaa tattgactct ttggcaatca tgatgcggag
     2821 catgtccttt aacaacggaa gcgaggccat caaattgttg ttcaacttgg tgattccagg
     2881 ctcggtggtg gtggtcgcca tcgcgtcaat gtcagctttg actgccagat agcgtttggt
     2941 gccctgatcg gcgccaggga gatatccgcc gttatagagg ttggctgcct gccaggtgag
     3001 aatttcactc agcggtgtcc cccccgtcac accagtaatg ttggtgtaat caaaatcgtc
     3061 tatcggaatg accgtcgttg ccagaaacgg aaagtatccg gccccataac ttaagccagc
     3121 ttctccgaca tcttcacgaa atcggttgat aatgtcttca taattctctt tggtggccag
     3181 tggagtgcca tacacatcca gaatggccat tcggtctttt aactcggcag attgttccag
     3241 cattctgcgg gtgactgcct ggaaatcagc cgatacccag cctggttctt tccccccggt
     3301 tggagctaac aaaacggcat ccggcacaac cagcatggtt ggcccaacct gttctttgat
     3361 cacccccaac ccttcgagga gcttttcttt gctgatgctg gtggtgcctt catacgtgcc
     3421 tacggagaca acaaaacaat tcccgccacc attggcataa aacaggcgaa gactgttgta
     3481 gagattgaat ttggtgcttc ccgtaggaaa aacaaggttg aaatatttcg tggttccatt
     3541 cggaattggg tacggaggtg gggttgtggg ttgcgttggg ggctcaacaa cctggaagtc
     3601 atattcgtga tttttgattt tgggttgatc cgtaacttcc tggatctgat agaccggatc
     3661 atattccttg ccaaagattt ccacaaaatc aaccatcgaa ccgattttaa tcggcttcat
     3721 aaagacaggt tttccactga tctccgcttt cttggtatac ccgataaaag ccggtacagc
     3781 ggtttgaaca ccaacaattg acggcggaaa ggcggaaagt tcggtgacat agacaccggg
     3841 cgttctgcga taaacagtag ctggcataag aacctcattg gtcggttaat ggacagtcag
     3901 gtttagagag cgacaaaaat gtcagaaaaa ttctgaattt tcggtacagg gtcaagttgg
     3961 gtttcagtca gtgtcccgtc cccatcgaac gggcagagct gggcgatgag ggtttggcag
     4021 ttgggagaat ccgcagcatc cggacataaa ctatttcgca gtttgaggca ggtggcggct
     4081 ttcgtttcag gtaaaacctg ctgcatagaa gccactggaa gccggtcaat caaaatattt
     4141 tcctggacca tcaacccgtg ctttccccac aattgaaaga tgaagtcgga gtgttgctgc
     4201 aactgaatcg gcaccgttga aacaaagcgg taggctatcg aaccgttggc aagcttcact
     4261 cgacaggggc cggcaaattc aatcggatcc cgatctggag tcacatcttt gatttgcagg
     4321 ttggtaagtc gttcttttga ccgctgtgga atcacaaaat agttccagta ggttgagcgg
     4381 gctttgaatt tgatttcata ggtgacgggg acaattccga tggttccatc cggcaatggt
     4441 tgaaccgggt agtgatctgg atattgaccc gtggggctgg taaacagaag gttgatgaaa
     4501 caaaaaggca cgggagaggc aattgtgtac agaacttctt gttgatcaat aatttgccca
     4561 cctttggcta cctgctcgat ggtatattta tcttcaggaa gcactgaaaa attaatatag
     4621 agcagatccc ggcatacctc ttcgcctttt cctgaggcgg catctttaca ggtaatggca
     4681 ttgggggatt ttttcagcaa gagttctttt ggaaaacacc gtggaaaaca aaggacttcc
     4741 tcatttgaaa tagcccgaac tttaactttt ttcgctgttg gtggcaccac aaccggaacc
     4801 tggatcggtg tcacttccag agtttcgcgt tcggtcacaa acgtctcggg atgcaggacg
     4861 atttgttgtt ccaaccgacg gtgggatctt tggtttgtaa agtaaaagtt ctcaagcgcc
     4921 gggttggtct taatgttgat attggtaaag ttgacaaaga actgattggt taagacgaac
     4981 acaaacgaaa gccgggtcca aatgccaggg actcccggat cagtctgccg ccctaaatat
     5041 tgaattaagt cctcttcttc cctggtattg tagaagacag aaaacccgcc gggttctgct
     5101 ttaaaaacca gcccgatgct atgcatcagg ctggcggtgg acggggttgg aagcacctgg
     5161 aagtccaggc aaaggtcgtc ttcgccgttg taataggaat ggcgcaccgt aacctgcatc
     5221 aaccgctgaa agtaatatcg gcgaggttcc tcacggtctc tgagaactcc ttttttctcc
     5281 cgttggggat tgcggagtac ccgctcagtt gtccgattcg gaaaaagagg gctgcgcata
     5341 gattcacctt ttcggtgtcg aaatgtgcga tggttacagg ttgatgccgg tcttgaaaat
     5401 ctatccaaat gaagtcttta tggcggtgat aactgctgct cgaccactcc gggtgtcaag
     5461 tggcaaggct ggtgagttgg tacctggatc ccagggatcg gcggggtttc gaccagttcc
     5521 aacaccaact gcatacagcc caaacccatc gttatcccag taatagtttg ggaagccgca
     5581 ggcaggcggg ccaccgtacc aggtgtacac attcggcata ttgaaacttc cgccagcgcc
     5641 gccattcggg gcgttgggac cggttgccgc cgggcaggtg aagttggccg cgatctgata
     5701 gccggattga ttccaccact catcgcaaaa ctcaaaatag tacagcccaa ggttcaatga
     5761 ttcggcaaaa gctgccggca gcatcgtggt gagcaccgtt gccacattgg cctccgtggt
     5821 cgagtcggaa taaatctccg ctggattaca cgtgttggcg gtggttcggc tggttgaggg
     5881 gaacccatat tcagtcagaa tcactggttt caaggctgca cccgtcaaac ttccgtagcc
     5941 ttgaaagacc gaggaaaaac tctgtggctg gtaggtgttg acaccccaaa aatcaattga
     6001 aggacaggtt gccatataag atgccgcatt ctggcagatt cctgggtcat cgtggttggc
     6061 aatcccgacc agcttatcgg gtgccgccgt ttttgcaatc tgcgccatgg cctgcacctg
     6121 gccccaccag taagttgcat ttgcctctgg accataggtg tcaacgctgc cgttatctac
     6181 ttcattggca atagtaaagc caagtactgc cggatgattg gctatttcag aaacagttgt
     6241 ctgaagattt tgagtccacc aatcagctga aggttgaggt gtttgaccca ggacaaacaa
     6301 cgattgtggc aacggaatac cgaccaggac atacagcccg ttttcataac aggcgtcaag
     6361 aaatgaggta tgggtaaaga tctgggctgg aggtgccggc ggaagttgcg tatcaatgaa
     6421 gcaatacacg cgaattgtgt tggccccgag cgctttaatg agaggcaaat catttgacca
     6481 ggtgccgctc cagttggtta ctccggtgta agcatcccaa aaccagtcgc caatgttcgg
     6541 cccatatgag tttgagccac caatcgggca gggtgaatag caaacccctt tcataacgaa
     6601 agggtctggc gagccgccgt tgacggaaac cttgagggat gccgtgagtg tcgaactatc
     6661 aaccgaacat gtccagacag tttgatcctg gttgctcatg cgatctcctt tgaaattgga
     6721 aaatgagagt tgagaagaac cctcgtatca ggggcggtgc tgatttttgc agattgtttg
     6781 aatcggttga aaaaccctaa acttctcctg gagtggtaac tcctttggct gcagagactt
     6841 ctgcttgagg catatcgctt tggaacggta gcagccggac cttgtaatac a
//
LOCUS       DAOQDO010000033         6797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6518_length_6797_cov_3.451201, whole genome shotgun sequence.
ACCESSION   DAOQDO010000033 DAOQDO010000000
VERSION     DAOQDO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6518_length_6797_cov_3.451201"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..3342)
                     /locus_tag="PKZ53_01065"
     CDS             complement(61..3342)
                     /locus_tag="PKZ53_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39051.1"
                     /translation="MLHKEQEEWILQNAVAKGLISQGDLRALEDSIGHAITFEGLPVW
                     SERIDYLVREGRLNQATVESLAIEAGTHNPTLGDSATGLAHAETIPMSGSHRLSPINS
                     DGFPVEKLEHYEFLHLLGHGGMGVVYKARDRRLNRVVALKFIRGSDPRQVQRFTQEAR
                     AQAQIDHANICKVYEVGQVDGNPFIAMQFINGPSLRDAQSDMTIEQKVTVIRDVADAL
                     QAAHRQGIIHRDIKPTNIMVEQLEDGRYRPFVMDFGLARDMGANDGLTQTGFIMGTPS
                     YMSPEQARGEVKLVDRRSDVYSLGATLYDLLSGQPPFDGTTAMGVMLQVISREPVPLR
                     KHLPGIPVDLETITLKCLEKAPHRRYDSAHALAQDLQAYLDGDPIQAQQASRWYRFVK
                     KARKNKVATGFALLAFLVFVVMTVVVVRERWQASVQAQLSQEFGESAKEIDALLRFAH
                     LMPRHDTRRERRLVQEQISRLERRMQETGSVSQGPGNYALGRAYLALNDYDRAWEHFQ
                     IAQQSGYQSREFSYALGLVLGELYRKHLEEAERSPTVAIRERRKRELEDQFLTPALNN
                     LKQYSQTEDTRQRGHSDYIKGLIAFYTRDYPLAIQKAQSAFEQVPWLYEAKKLEADVA
                     VARGEEKSDKGDYAGAIEEFTQAGQAYQEAIRIGQSDATIYEGDIQRWFGMVTVESAQ
                     GTLTDKTFQNLLEACDKALVINPEAGVIFNRKAVCYWRMGERQFLQGEDPRQALDQSV
                     AFAQQAIALGTTRANVYRNLGIAYSIRGEYEMYHGLDPQDSFNRSITSLNQALRFTPN
                     DTGAYNELGILYNSLSYYDMEHGKDPRQNIRQGIETYRKLIQIDPTYTPAFTNISNAY
                     STLGDFEMQIGLDPTNSLTQCLEAINEALRLNPNSGADNTNLGGVHNLMSEYRLKQNQ
                     DPMPTVLQTQKAVGKSIEINPTNPSPYILGAEAEILAARWVIRNNQSPESYFAKAESM
                     FQVAQKLNSEDDYLYFARSELYRWKAEWQLQHNQNPLPAIQTGLAELDRALQINPTNS
                     EVIARKGVLYRLQAKATRSATTQAMAIKQAVENLEKAFQLNSLLKYEYGQYLTESQSS
                     VVSRQ"
     gene            3562..4443
                     /locus_tag="PKZ53_01070"
     CDS             3562..4443
                     /locus_tag="PKZ53_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39052.1"
                     /translation="MIENQYFPDVQPSVPEQRLSRDIPLTEADIKSFERFYQDFLGKR
                     PEDRMAFLKALSIYRRGDLREYVHDAVVFHNRFKQLMPSIKQRDLLLSFPTVTRNVHN
                     LIFDFSESYGGITGQTNVSMAKTQVTEHLDATEMFRLDLDKALETMPSVLPDDQALIS
                     GFIEKLKQGLPATETFRFLIVILADHAFLGVIPGYERGSTLVVGKIREFLEQRFGARM
                     EDHIVEFFVVGGGYLRSDGDMVVLGGHNSVFDPQFAEPTNPAVQEFHHRFLEMKFHLA
                     QQILTTEFPGLKVRFQL"
     gene            4522..4767
                     /locus_tag="PKZ53_01075"
     CDS             4522..4767
                     /locus_tag="PKZ53_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39053.1"
                     /translation="MKTQSIQRYLPGFYILFGLGYIGYWVMVYPKSPLLFLVLALLLT
                     LMGIWRLLRIRRPVVVKPESIHVVIDDGNERAKASRE"
     gene            4814..5476
                     /locus_tag="PKZ53_01080"
     CDS             4814..5476
                     /locus_tag="PKZ53_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39054.1"
                     /translation="MSINALPLNLAQCLDTLVSNAGDGSLMDASGERRIWLTRGFWQA
                     LQASTIQSLAGNHGKSVLYAIGRYWGERSFPGLQHRFTGQSVGAGKGDPKGKRALIKA
                     CDALWSEGGWGHCDLVYVYGLTLVTFYHSPVALNLEPAPEPVDWVIAGMLAGVFSVFE
                     GIRLEAVEICCRASGAEYCQFIIGSEETAARVRKLLVPGMSHPDLLQRLRDDTSGMRD
                     EL"
     gene            5566..6225
                     /locus_tag="PKZ53_01085"
     CDS             5566..6225
                     /locus_tag="PKZ53_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39055.1"
                     /translation="MIADLPTLNFEPRTWREWAAADPETGTVTTLNGNPLALTGSDFI
                     LAVLQAAETLGDQGPGGWLYEVGVGWGQRHCLELERVIQADRGHPVEVSAMALPIFIS
                     TLNQSLGELGWGKVEVVEEEGHAFAIVQSSPVAAAVKQAHHPICHLYAGFLAGVFGQA
                     AQIDLACIEIGCQASGLDQCRFFIGSQIKIDLVASWLKTGKTMTEIRRHLSEQLGVTA
                     Q"
     gene            6288..6692
                     /locus_tag="PKZ53_01090"
     CDS             6288..6692
                     /locus_tag="PKZ53_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39056.1"
                     /translation="MSTKIYRETQEAAQLLETLRGRRTPTIQPATREELELVIEQLRR
                     PSRSPRTTMVVSPPVSSGTDETGWPAGQATTTVTLESQPDCLNEATADTAPDTGSVAP
                     PSLTPSNVTTVHKIESVADFFRRMILSPKTRG"
BASE COUNT         1526 a         1682 c         1838 g         1751 t
ORIGIN      
        1 ataactgact caaatagaac taaatacact tgacaccaat tccaaaatcc caacgcctga
       61 ctactgacga ctgacgactg acgactgact ttctgtcaaa tactgaccat attcatattt
      121 tagtaaacta ttgagttgaa atgctttctc aagattttca actgcctgtt tgattgccat
      181 ggcctgggtt gtggctgaac gggtcgcttt tgcctgtaag cgatagagca cacctttgcg
      241 agcaatgact tcagagttgg ttggattgat ctgcagcgcc cggtcaagct cggccagccc
      301 ggtttggatt gccggcaatg gattttggtt gtgctgtaat tgccattcag ctttccagcg
      361 atagagttca gatctggcaa agtagaggta atcatcctct gaattcagtt tttgcgcgac
      421 ctggaacata ctttcggctt tggcaaagta ggattccggc gattgattat ttctaattac
      481 ccatcgtgct gccagaattt cggcttcagc ccccaggata tatggcgatg ggttggttgg
      541 gttaatttca atggatttgc caaccgcttt ttgagtttgc agcaccgttg gcattggatc
      601 ctgattttgt ttgagccggt attcgctcat cagattgtgg acaccgccca aattggtatt
      661 atccgcgccg gaatttggat tgagccggag ggcttcgttg atggcttcaa ggcactgcgt
      721 gagtgaattg gttggatcca gaccaatttg catttcaaaa tctcccagcg tgctataggc
      781 attggagata ttggtgaagg ctggggtata ggtcggatca atctgaatca gtttccggta
      841 ggtttcgatg ccttgcctga tgttctgacg ggggtctttc ccgtgctcca tatcatagta
      901 actcaatgaa ttatagagaa tcccaagctc attgtaggca cctgtgtcat ttggcgtaaa
      961 gcgcagtgcc tgattcaaac tggtgattga gcggttaaac gaatcctgtg ggtcaagacc
     1021 gtggtacatc tcgtattcgc cgcgaattga ataggcaatt cctaaattgc gatagacgtt
     1081 ggcccgagtg gttccgagcg caatcgcttg ctgggcaaaa gccactgact gatccagggc
     1141 ctgacgcgga tcctcaccct gaagaaactg ccgttctccc attcgccagt agcacaccgc
     1201 cttccgatta aaaatcacac cggcttcggg attgatgacc agcgctttgt cgcaggcttc
     1261 cagaaggttt tggaatgttt tatcggtcag tgttccctgg gcgctttcga ccgtgaccat
     1321 accaaaccag cgctgaatat ccccttcata aatggtggca tcactttggc caatgcggat
     1381 ggcttcctga taggcctgac ccgcctgggt aaattcctct atcgcgcccg cataatcgcc
     1441 tttatcggat ttttcctcgc cgcgggccac ggccacatca gcttccaatt ttttggcttc
     1501 atacagccag ggaacctgtt caaaagcgga ttgggctttc tggatggcga gtgggtagtc
     1561 cctggtatag aaggcgatga gccctttgat atagtcgcta tgtccgcgct ggcgggtgtc
     1621 ttcagtttgg gaatattgct tgagattgtt gagtgccggc gtcaggaact ggtcttccag
     1681 ttcacgttta cgtcgttccc gaatcgccac tgttgggctg cgttcagctt cttccaggtg
     1741 ttttcgatac agttcgccaa gaaccagccc gagggcatag gaaaattcac gcgattgata
     1801 tccgctttgc tgggcaatct ggaagtgttc ccaggcccgg tcataatcat tgagtgccag
     1861 gtaggcacgc cccagggcat aatttccagg gccctggctg acgctgcccg tttcctgcat
     1921 tcggcgttca aggcggctga tttgttcctg aaccagcctt cgctcgcgcc gggtatcgtg
     1981 ccggggcatc aggtgggcaa aacgcagcaa ggcatcaatt tctttggctg attcgccaaa
     2041 ttcctgtgac aactgcgcct ggactgaagc ctgccagcgt tcgcgaacca ccaccaccgt
     2101 catgaccaca aacaccagaa acgcaaggag ggcaaatccg gtggccactt tgtttttgcg
     2161 cgcttttttg acaaatcggt accagcgtga tgcctgctgc gcctggatgg ggtcgccgtc
     2221 aaggtaggcc tgcaggtcct gggccagggc atgggcagag tcatagcggc gatgtggggc
     2281 tttttcaagg cacttgaggg tgatggtttc aagatcaact ggaatcccag gcagatgttt
     2341 gcgcaagggc accggctcgc gggaaatcac ctggagcatc acccccatgg ccgtggtgcc
     2401 gtcaaatggg ggctgccccg agagcaaatc atagagcgtt gccccaaggc tatagacatc
     2461 ggaccgccgg tctaccagtt tgacctcacc gcgagcctgt tcgggtgaca tataggatgg
     2521 ggttcccata atgaacccgg tctgggtcag gccatcgttg gcgcccatat cgcgggcgag
     2581 cccgaaatcc atgacaaagg ggcgatagcg accatcttca agttgttcga ccataatgtt
     2641 ggtcggtttg atatcgcgat ggatgatccc ctggcgatga gcggcttgaa gcgcatcggc
     2701 cacatcccga atcaccgtca ctttttgttc aatggtcata tccgattgag catcgcgcag
     2761 ggaaggccca ttgataaatt gcatggcaat aaagggattt ccatcaacct gaccaacttc
     2821 atacactttg cagatgtttg catggtcaat ttgagcctgg gcgcgggctt cctgggtaaa
     2881 tcgttgaacc tgccgggggt cgctgccacg aatgaattta agcgccacaa cgcggtttaa
     2941 ccgtcggtca cgtgctttat acaccacacc catgccaccg tggccgagca ggtgcagaaa
     3001 ctcgtaatgc tctagttttt cgacggggaa cccatcggaa ttgatgggcg atagccggtg
     3061 tgacccggac atagggatgg tttcggcgtg ggcaaggccg gtggccgagt cgcccagggt
     3121 cggattgtgg gtcccggctt caatcgccag cgattcaacc gttgcctgat tgaggcgtcc
     3181 ttcacgaacc aggtagtcaa tccgctcgct ccaaaccggc aatccttcaa aggtaatggc
     3241 atgcccgata gaatcttcca gcgcgcgcaa atctccctga ctgatcaacc ccttggccac
     3301 tgcattttgt agaatccatt cttcttgttc tttgtgcagc attgcaacct gcctgtgtca
     3361 gtcgtggtca gagcgttccc cagttctgga caaaagatga tcatttcttt aatcttgcag
     3421 gagttccgca agaagttcca tgattttttc attgacgaca aatatcttta aataaataac
     3481 ttaaatcaat atccatctct ggggtgaagt ttccccacca gccgcatttc aaaccatgat
     3541 tgcaggtaga aggaagtctc catgattgag aatcagtatt ttccagatgt ccagccgtca
     3601 gttccggaac aacgattaag ccgggatatc ccgctaaccg aagctgatat caagagtttt
     3661 gagcgatttt atcaggattt tctggggaag cgtcccgaag atcgaatggc ttttctcaaa
     3721 gccctgtcga tctaccggcg aggtgacctg cgtgagtatg tccatgatgc tgtggtcttt
     3781 cataaccgat ttaaacaact gatgccaagc attaaacagc gggatttatt gttgagcttc
     3841 ccaaccgtaa cccgcaatgt tcacaatttg atctttgatt tctctgaaag ttacggtggg
     3901 attaccgggc aaaccaatgt gtcgatggcc aaaacacagg ttactgagca tttggatgcc
     3961 actgaaatgt ttcggttgga tctggataaa gcacttgaaa cgatgccttc ggtgctcccc
     4021 gatgaccagg cgctgatcag tgggtttatc gagaaactga aacagggcct tccggcgacg
     4081 gaaaccttcc gctttttgat tgtgattttg gctgatcatg cctttctcgg ggtgattcct
     4141 ggttatgaac ggggaagcac gctggtggtt gggaaaatcc gggagtttct tgaacaaaga
     4201 tttggtgccc gcatggaaga ccacatcgtt gagttttttg tggttggggg cggatatttg
     4261 cgatctgatg gtgatatggt cgtgcttggt ggtcacaact cagtttttga cccgcaattt
     4321 gccgagccga ccaacccggc ggttcaggag ttccatcacc gatttttgga aatgaaattc
     4381 catctggctc aacaaattct gactactgag tttccggggc tcaaagtccg atttcagctt
     4441 taacgaaagc ggtgtcaagc caccgcactc cacacaattc cgaaagcggt gtcagaccac
     4501 cgcactccac acgcatttct tgtgaaaact caatcaattc aacgctattt gccagggttt
     4561 tatattctgt ttggcctggg gtacatcggc tattgggtga tggtgtaccc caaatctcca
     4621 ctgttgtttc tggtgcttgc cttgttgctg accttgatgg gaatctggcg gttgctccgc
     4681 atccgacgtc cggtggtcgt caaacccgag tcaatccacg tggtcattga tgatgggaat
     4741 gagcgggcaa aagcgagtcg cgaatagtca gtaccacctg cgtgagcggg tgggcagaaa
     4801 gcagtacatc tctatgtcaa tcaatgcttt acctctgaat ctggctcaat gcctcgatac
     4861 tctggtctcc aatgctggtg atggcagcct gatggatgcc agcggcgaac gaagaatctg
     4921 gctgacccga gggttctggc aggcactgca ggccagcacg attcagtcgc tggcgggaaa
     4981 tcacgggaaa tcggtgctat atgccattgg ccggtactgg ggggagcgat ctttcccagg
     5041 gttgcaacat cggttcaccg gtcagtcagt cggtgccgga aagggtgacc caaaggggaa
     5101 acgcgccttg atcaaagcct gtgatgcgct gtggagcgaa ggtggctggg ggcactgtga
     5161 tctggtgtat gtctatgggt tgaccctggt gaccttttat cattcacccg tggcactgaa
     5221 tctggaacca gcgccggaac cggttgactg ggtgattgct ggaatgctgg ccggggtgtt
     5281 ttccgttttt gaaggaattc gattggaggc ggtcgagatc tgttgccggg ccagtggtgc
     5341 cgaatattgc cagtttatca ttggctccga agaaactgcc gccagggtgc gaaaactgct
     5401 ggttccaggg atgtcccacc cggatttgct ccaacgcctt cgcgatgaca cgtcaggtat
     5461 gcgggatgaa ttataaatga taagtgagat atcaggttca atatcaatga gttcgctcat
     5521 ttatacttga gaactcagaa tttggatact ggaggatttc cacctgtgat tgccgattta
     5581 ccaactctga actttgagcc tcgaacctgg cgtgaatggg cggcggcgga cccagaaacc
     5641 ggcacggtga cgactctgaa cgggaacccg ctggcactaa ctggttcaga ctttatcctg
     5701 gcggtgcttc aggcggctga aaccctgggc gatcaggggc cgggcggctg gctttatgaa
     5761 gtcggtgttg gctggggaca acggcattgt cttgaactgg aacgcgtcat tcaggctgat
     5821 cgaggacatc cagtcgaggt atcggccatg gcgttgccaa tttttatctc gacgttaaac
     5881 cagtcactgg gagagcttgg ctggggaaaa gtcgaagttg ttgaagaaga aggacatgct
     5941 tttgccattg tgcaatcttc cccagtggca gcggctgtca aacaggccca ccatcctatc
     6001 tgtcatctat atgccggatt tctggccgga gtgtttggac aggctgccca gattgacctt
     6061 gcctgtattg aaattggctg ccaggcaagc ggacttgacc agtgtcggtt ttttattgga
     6121 agtcaaatta agattgatct ggtggcatcc tggttgaaaa cagggaagac gatgaccgaa
     6181 attcgtcgcc acctcagtga acaactcggt gtgacagctc aatgaccgtc cccaggtctt
     6241 cagaggacag tgctggtttg atcatttatt caggagaatt cacgggaatg tccaccaaga
     6301 tttatcggga aactcaagaa gccgctcagc tattggaaac attgcgcggt cgccgaaccc
     6361 caaccataca acctgctacg cgtgaagaac tggaactggt gattgaacag ttacggcgtc
     6421 cttccaggtc gccacgaacc acgatggtgg tatcgccgcc tgtatcatct ggaactgatg
     6481 aaaccgggtg gccggctggt caggcgacca ccacggttac cctcgaatct cagcctgatt
     6541 gtctcaatga agctacggct gatactgccc cagatactgg atctgttgcc ccgccatcat
     6601 tgaccccgtc aaacgtgacc actgtacata agattgaatc ggtggctgat ttctttcgtc
     6661 ggatgatcct gagcccaaaa acaaggggtt gaagaaatcc gggctccggg cttggggcgg
     6721 aagacaacgg gctgaagcag tagttttatt tcatccctca tccttcatcc ctcatccctt
     6781 caattgcccc gagcttg
//
LOCUS       DAOQDO010000034         6768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6566_length_6768_cov_3.852674, whole genome shotgun sequence.
ACCESSION   DAOQDO010000034 DAOQDO010000000
VERSION     DAOQDO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6566_length_6768_cov_3.852674"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKZ53_01095"
     CDS             complement(<1..853)
                     /locus_tag="PKZ53_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNJ39057.1"
                     /translation="MNTFARQSQRNPGQNGTVPADAVQKKQTLHRSASTGQAQRTIPE
                     SLNQSPQVQTQLQLQNSLNNSPQAAIQAKFAEALANRDQPLQRMCDACSEEEKKVKPK
                     PSGMIEDEDAVAQTKSANSPGSSQNSFVENRTGLPNQLKSGIEALSDVSLDDVNVHYN
                     SSKPAQLQALAYTQGSDIHVGPGQEQHLAHEAWHVVQQKQGRVQPTTQLQDVAINDDF
                     GLEREADVMGEKALQMKATGDFNLPNYQAQGPIQRKLDEFPLTASSETDFDEEDVLQA
                     RLEPFPES"
     gene            complement(938..2581)
                     /locus_tag="PKZ53_01100"
     CDS             complement(938..2581)
                     /locus_tag="PKZ53_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ39058.1"
                     /translation="MSSVAYQAPKEPQLASADTPSLTELIRQPAVQPGEAQPVSGGTI
                     QLAQSLNQRPNVLSQLLLQRKFNQGSGGQIQSQLTERLAQRKEKIVSYSGGNVVQLMK
                     ANEIKLTQGLKPEHPDIIYRIPVGAANLPPVYRYGKFVGRSGGALMGLYLFKDKTGVF
                     PVVGGTEIEISSGGSLEENRTAFGLDERNEEQIKAAVEAGYRVFDAAESYGNTDVLAK
                     VLAESPNLTRNDVKIVYKFRPGEPAQTIYEKLKGVAEKFGGYLDVVLLHDVEESQQNI
                     EGAFKAIQVLKKEGIVKAVGASNIKADSETFAKIASAEDEQDSIYKVDVLSNRFSPKG
                     PDEKIREFAKSRGITYLGFGLMGGVVNDEHFQKWAQELGYTASQLLFAWAQAHGTIPV
                     ASSKNTEVIGQNFLQAQTPVPLEVVQQVEEYWAKRREEAQGLGDEVLAEIPPHISKLL
                     TGSQIEKFALGQIDDSTWRGQASNKQTPIGKNIFFLTAMLQDPPATVPPLQSIRRDLW
                     GKSVSDLVSGLATSNSCNTLFCIDAILKSLRYPGNFQNL"
     gene            complement(2662..3861)
                     /locus_tag="PKZ53_01105"
     CDS             complement(2662..3861)
                     /locus_tag="PKZ53_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39059.1"
                     /translation="MYTINFVNQNTDIPTAYVTFIQATQVVNNVTELVGPALNATGGN
                     GTTLENGVSYSLDSIQNTISMDPSNWQGHMYLSDAALTPPTSGGLPVDMAYTLTTDTN
                     RYQMIEFAGSATSATTDITYINWYSIPLQMETTTSNTGGNSRGVPSSQTALSDLISTL
                     AGLSGNSSTTVVKNSSNETVRVNSPNAGNPSWLPLYESFRDYLVSVFIDSTDSILLSN
                     SYSGIQNPPSLDFQAQTYSASSVTYNGLTLNITGTTSVLGNFTMTATMVFADFSSVIY
                     LAVMNYEWSYDGQNGSASNPTGNTGDNNVFSAISRDLMAGFNFGFVGSSQYGSQPSSA
                     WQQASDSELFSAIQPNKPYYNPWANAINGCFSDVYSFPFNDFLSNFSPLLTTNDGDIL
                     TVTLLNP"
     gene            complement(3898..>6768)
                     /locus_tag="PKZ53_01110"
     CDS             complement(3898..>6768)
                     /locus_tag="PKZ53_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39060.1"
                     /translation="DLTNLSLSNTDGAIDPTSPYAVFGGLPVVGSYLEIQHSELFCKD
                     IKSLSVGIKWFNLPQNETGFKGYYKDYVIGLDGLPSPTPLFENQYFEGVFRVKNPGTW
                     VLSDVSSCPETPSNSVEVFLFRTKPDCDTQEPTPDGQLCRKSQFTTLQICPAGPPDYY
                     DPNQSALRLEFSEPPYGFGNAIYAQNVLNAVIEDLPDSEKCEEKCLAECQMLKAAAQL
                     IEAGLLSLADCQMATCSDTPGCLEKSLTNTVTYICTLVITCLETCEKGSSTEVEQLRQ
                     LLIQVKAEQKLLGTSQIQTLRETLNRFYTGNIQTRLKQPAKCFKCLSLVEVCLHLLAD
                     IIAYQLQAQANFDELKRCLRLCKSGLEKISVTCLEQCLAMPVSTDPKCIIECVQNCLN
                     SLFQQSFQKLETCLGKISDPQAAQVLTQIKEKIQSLRENPSITQTQFFQECLGFLTSI
                     LQQDPLPVPVKLCLEAFEKRIDACLCLENCVVDFAAQSKANFETLEKSLVDRQTEAET
                     AFEECYELCVKNCMSLKNELKYPNEPYLPQAEKITVTYEASCYIPTTGQNSADGMFFH
                     LLPFAGYQQPELISTLLPVFPNQGNLYLGFSGLPSSKLLTLLFQMVAENTGDQPVTLP
                     QVKWNYLSENRWHEFKETELRQDTTNGLQNTGITGLVLPWFDPTGTTILPADYQWLWV
                     SAKNRAPAFPTVIGIYPHAMLATWQNIDNTGETLKTPVPAYTISSSVQDLPDIGSIVQ
                     PMPSFGGRPPENDRTLTIRIGERLQHKDRAILNWDYERLVLERFPTIWKVQTLPARNL
                     KAGNSPGDVLVVVIPGPDSDETMEPTTPTVSSAMLNQIQTYLASRNSPFINLQVVNPI
                     YVLITVTTTVQFLETEDIGDCINRLNTDLINYLSPWFYDVDRAAKGGDYATEANISEF
                     IQTRPYVEVMMSISLNHSPKPRTLDWYFLTSAKQHQISTSPE"
BASE COUNT         1552 a         1575 c         1775 g         1866 t
ORIGIN      
        1 cggattcagg aaatggttcg agtctggctt gtaagacatc ttcttcgtca aaatcagtct
       61 cggatgatgc cgtgagcgga aattcatcca gttttcgctg gattggtccc tgggcttgat
      121 agttgggaag gttgaaatca cccgttgcct tcatctgaag tgctttctca cccatcacat
      181 ctgcttcgcg ttcaaggccg aaatcatcat tgatggcgac atcctggagt tgcgtcgttg
      241 gttgaacccg gccctgtttt tgctggacga cgtgccacgc ttcgtgggcc aggtgttgtt
      301 cctgaccggg gccgacgtgg atatctgaac cttgagtgta ggccagcgcc tgcaactggg
      361 ctggcttcga tgagttgtaa tgcacgttga catcatcgag tgagacatca gaaagcgctt
      421 cgatccccga tttgagctgg ttgggcagac cggtccggtt ttcgacgaat gaattttgtg
      481 acgaaccggg tgaattggcc gatttggttt gggcaacggc gtcttcgtct tcaatcattc
      541 ccgatggttt cggctttact ttcttttctt cttcggaaca ggcatcgcac atccgctgga
      601 gcggctgatc ccgattggcc agggcttcag caaatttggc ttgaatggca gcttgcgggc
      661 tgttgttcaa cgagttttga agctgaagct gggtttgcac ctgggggctt tggtttaacg
      721 attcggggat ggttcgttgg gcctgacctg tgctggcaga acgatgcagt gtctgttttt
      781 tttgaactgc atcagccggg acggttccat tctggcctgg attgcgctgt gattgcctgg
      841 caaacgtgtt cataattctt cccctttaaa tacaaatggc gatcaacttc ggtgctcgac
      901 ggcgagagtc aaaattttgt gtgatgaaag gacctgatta gagattttgg aaattgcctg
      961 gatagcgaag tgatttcaag atggcatcaa tacaaaacaa ggtattgcaa ctgtttgagg
     1021 tagcgagacc tgaaaccaaa tctgagactg atttccccca cagatccctg cgaatactct
     1081 ggagtggcgg aactgttgcc ggagggtctt gcagcatcgc cgtcagaaag aagatatttt
     1141 tcccaatcgg agtttgctta ttcgatgctt gacctctcca ggtactatcg tcaatttgac
     1201 caagcgcaaa tttttcaatt tggcttccgg taagtaattt cgatatatgg ggtggtattt
     1261 cagcaagcac ttcatccccc aatccttgtg cctcttctct tcgcttggcc cagtattctt
     1321 cgacctgctg gacaacttcc aacggaacgg gagtttgtgc ctggagaaag ttctgaccaa
     1381 tcacttcggt attctttgac gaagcaaccg ggatcgtgcc gtgagcctgg gcccaggcaa
     1441 acagaagctg actggcggta tatccgagtt cttgagccca cttctggaag tgttcgtcgt
     1501 ttacaacccc gcccataagg ccaaacccta aataggtgat tcctctggat ttggcaaatt
     1561 ctctgatttt ttcatcgggt cccttcggtg aaaagcgatt ggaaagaaca tcaactttat
     1621 aaatcgaatc ctgttcatct tccgctgagg caatcttggc aaacgtttcg gaatcagctt
     1681 tgatattgct ggcgccaacc gctttgacaa tgccttcttt tttgagaact tgaatcgctt
     1741 tgaaggctcc ctcaatgttt tgctggcttt cctcaacatc gtgaagcaaa acgacgtcca
     1801 gatacccacc aaacttttct gcgacccctt tgagtttttc ataaatcgtt tgagctggtt
     1861 cacccggtct gaatttgtag acaattttga catcgttgcg cgtcagattc ggggattctg
     1921 ccagcacttt ggccaaaaca tctgtgtttc cataggattc agccgcgtca aagactcgat
     1981 atccggcctc caccgctgct ttgatctgct cttcatttcg ttcatcgagt ccgaaggcag
     2041 tccgattttc ctccaatgat ccgccactgg agatttcaat ttccgtcccg ccgaccactg
     2101 gaaacacccc agttttatct ttaaacaggt acaaccccat caacgcgcct ccagaccgcc
     2161 caacaaactt cccatagcga tagactggcg ggaggtttgc cgcgccaact ggaattcgat
     2221 aaatgatatc cgggtgctcg ggttttaagc cctgggtcag cttaatttca ttggctttca
     2281 tcagttgcac aacattacca cccgaatagc tgacgatttt ctctttcctt tgcgccagcc
     2341 gctcagttaa ttgcgattgg atctgccccc ctgaaccctg attgaatttc cgttgaagaa
     2401 gaagctggga gagcacattt ggacgttggt tcaggctttg ggcaagttgt attgtgccac
     2461 ctgatactgg ctgggcttct cctggttgaa ccgctggttg ccgaatgagt tccgtcaaag
     2521 agggggtgtc agccgaagcc agttggggct cttttggcgc ttgataagcc actgaactca
     2581 taacagtcct ttttgttatc ggcctgggcg tgttttttgg cacgcccagg ccagttccct
     2641 attgcacacc aaccgcaatc attacgggtt gagcagtgtg accgtcagaa tgtctccgtc
     2701 attggtggtc aggagcggcg agaagtttga aaggaaatca ttgaagggaa acgaataaac
     2761 atccgaaaaa caaccgttga tcgcattggc ccagggattg taatacggtt tatttggctg
     2821 gatggcgctg aaaagctctg aatcagatgc ctgctgccag gctgaactcg gctgtgaccc
     2881 gtactggctg cttccgacaa aaccgaagtt gaatccagcc atcaaatcac gggaaatggc
     2941 ggaaaagacg ttgttgtcgc ccgtgtttcc agttggattt gaggcagacc cgttctgccc
     3001 atcataggac cattcgtaat tcatcaccgc gagataaatc acagagctaa aatcggcaaa
     3061 aaccatcgtg gctgtcatgg taaaattgcc cagaacgctg gtggtaccgg tgatgttgag
     3121 tgtcaaaccg ttgtaggtca ccgatgaagc actgtaggtt tgagcctgaa agtcgagcga
     3181 tggtgggttt tggatcccgc tatagctgtt tgacagtaaa attgaatcgg tcgaatcaat
     3241 aaacacgctc accagataat ctctaaaact ttcgtacaac ggcaaccacg aaggatttcc
     3301 ggcattcggg ctgtttactc gaactgtttc attacttgaa tttttcacca cggtcgttga
     3361 actgtttcca gaaagtccgg ccagcgtcga aatcaaatcc gacaaagccg tttgggaact
     3421 gggcactccg cgagagtttc caccagtatt tgaggtcgtg gtttccatct ggagcgggat
     3481 tgaataccag ttgatatagg taatatcggt ggtggcgctc gtcgctgagc cggcaaactc
     3541 aatcatttga tagcgattgg tatcagttgt cagcgtgtac gccatatcaa ctggcaatcc
     3601 tcccgaagtt ggaggcgtga gcgcggcatc actcagatac atatggcctt gccagttgga
     3661 tgggtccata ctgatggtgt tttgaatcga gtccaatgaa tatgaaacgc cgttttccag
     3721 tgtcgtgccg tttccgccag tcgcattcag ggctgggccg accagttcgg tgacgttatt
     3781 gaccacctgg gtcgcttgaa tgaacgtgac atacgcggtc ggaatgtcgg tattttggtt
     3841 cacaaaattg atggtgtaca tgagtcctcc tgcttgtggt gaggctgtgg tgtggtttta
     3901 ctcaggactg gtggaaatct ggtgctgttt ggctgaggtg agaaagtacc agtcgagtgt
     3961 tctcggcttt ggcgaatggt tgagcgaaat ggacatcatc acttcgacgt aaggtctggt
     4021 ttggataaac tctgaaatgt tggcttctgt ggcatagtcg ccgcctttgg ccgcacgatc
     4081 cacgtcataa aaccagggtg ataagtagtt gatcaggtcg gtatttaagc ggttgataca
     4141 gtcgccgatg tcttcggttt caagaaactg gactgtggtt gtgaccgtga tcaatacata
     4201 gattggattg accacctgaa gattgataaa tggcgagttt cggcttgcca ggtatgtctg
     4261 aatttgattc agcatggcgg aactgacggt tggcgtcgtc ggctccatgg tttcgtcgct
     4321 atccggccca ggaatgacaa ccaccagtac atctccgggt gaatttccgg ctttgagatt
     4381 gcgtgccggc agggtttgaa ctttccaaat ggtgggaaag cgttcgagta ccagccgctc
     4441 ataatcccag ttcaggatcg cacgatcttt gtgttgcagc cgttcaccga tgcggatggt
     4501 cagcgtgcgg tcattttccg gaggccgtcc gccaaaggat ggcatcggct gcacgatgct
     4561 gccaatgtct ggcaaatcct gaaccgaact gctgatagtg taagccggaa ctggtgtttt
     4621 gagggtttcc cctgtgttat caatattttg ccaggttgcc agcatggcgt gcggatagat
     4681 tccaattaca gtgggaaacg ctggcgcccg gtttttggct gaaacccaga gccactgata
     4741 gtctgccggg agaatcgtgg ttcccgtcgg gtcaaaccat ggaagcacga gacctgtgat
     4801 cccggtattt tgcaggccat tggttgtgtc ctgcctgagt tctgtttctt tgaattcgtg
     4861 ccagcgattt tcagagaggt aattccattt gacctggggc aaagtgactg gctggtcacc
     4921 ggtattttcg gcaaccattt gaaagagcag cgtcagcaat ttcgaagacg gcagtcccga
     4981 aaacccaaga tagagatttc cctgatttgg aaacactgga agcaatgtcg aaataagttc
     5041 cggttgttgg tacccggcaa agggcaataa gtggaaaaac atcccatctg ccgagttctg
     5101 gcccgttgtc gggatataac aactggcctc ataggtgact gttatttttt cagcctgggg
     5161 gagatagggt tcatttgggt acttgagttc gtttttcagg ctcatgcagt ttttgacgca
     5221 cagctcataa cattcctcaa aggctgtttc ggcttcggtt tgccgatcaa ccaggctctt
     5281 ttccagcgtt tcaaagttcg ctttggattg ggcggcaaaa tcaaccacac agttttcaag
     5341 gcacagacag gcatcaatcc gtttttcaaa tgcttccagg cacagtttca ccggcaccgg
     5401 aagaggatct tgctgcagga ttgaagtaag gaacccaagg cactcctgga aaaattgagt
     5461 ctgggtgatg gaggggtttt ccctgagcga ttgaattttt tccttgattt gggtcaggac
     5521 ttgagctgcc tgtggatcac tgatttttcc caggcaggtt tccagctttt gaaatgattg
     5581 ctgaaagagc gaattcaggc aattttgaac acattcgata atgcacttcg ggtcggttga
     5641 aactggcatc gccaggcact gttccaggca ggtgacgctg attttctcca atcctgattt
     5701 gcaaagtctg aggcaacgtt tgagttcatc gaaattggcc tgagcctgga gttgataggc
     5761 aatgatatca gccagcaggt gaagacagac ttcaaccagc gaaagacact tgaagcactt
     5821 tgccggctgt tttaatcggg tttgtatgtt tcctgtatag aagcgattga gtgtttcgcg
     5881 caacgtttga atctggctgg tgcccagaag tttctgctca gctttgactt gaatcaggag
     5941 ctgacgaagc tgttccacct cagtgctgga tcctttttca cacgtttcca ggcaggtaat
     6001 caccagcgtg cagatatagg tcacggtgtt ggtcaagctt ttctcaaggc accctggggt
     6061 atcgctgcag gttgccatct gacaatcagc cagcgagagc aatccggcct cgatcaattg
     6121 cgctgccgcc ttcaacatct gacattcggc cagacatttt tcctcacact tttctgagtc
     6181 aggcaggtct tcaatgacgg catttaaaac attttgggca tagattgcat tgccaaatcc
     6241 atagggtggt tcgctgaatt caagcctgag ggcactctgg tttggatcat agtaatcagg
     6301 cggcccggct gggcaaatct gcagcgtggt aaattgtgat tttcggcaaa gctggccatc
     6361 tggcgtcggt tcctgggtgt cgcaatcggg tttggttcga aataaaaaga cctcgactga
     6421 gtttgacggt gtctcgggac aggatgaaac atcacttaaa acccaggtgc cgggattttt
     6481 aacacgaaac acgccttcaa aatactgatt ttcaaacaac ggggttggcg acggcagacc
     6541 atccagccca atcacataat ccttgtaata gcctttaaac ccggtttcat tctggggcag
     6601 gttaaaccat ttgattccaa ctgagagtga tttgatatct ttgcaaaaca gttcggaatg
     6661 ttgaatttcc aggtatgagc cgactacggg caaaccgccg aaaactgcat atggtgaggt
     6721 cgggtcaatg gcaccatcgg tgttggagag tgaaaggttg gtcaggtc
//
LOCUS       DAOQDO010000035         6767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6571_length_6767_cov_3.118743, whole genome shotgun sequence.
ACCESSION   DAOQDO010000035 DAOQDO010000000
VERSION     DAOQDO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_6767_cov_3.118743"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..3562
                     /gene="tssM"
                     /locus_tag="PKZ53_01115"
     CDS             68..3562
                     /gene="tssM"
                     /locus_tag="PKZ53_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HNJ39061.1"
                     /translation="MNWQSLPFKSWFHLLGLIVLYGLACLVAWLVAPWLGFSFVGQLG
                     LIGLILFTWLLTLVFEKVRRMYWPAETDQTQQLQPGNESMGDGPANLPGQGEAFAMLT
                     DWVETTCGRKTQDRFRQPWFGVIGPPASGKSALLLTSQLDFQLLPHQRPADLYTIAPT
                     TIGECYVSESAVYLDLPGHLVADTAQNAAWKHLCQWLGEYRDTRPVDGLVIVADAHRL
                     CSLAESDIEQEAKILRARLDECLQWWGHQFPLYLVFSHSDKLAGFAAFFEEYDRGERS
                     QVWGATIPLEQQSTGYALFDTEFGYLYEALMRRRLLRLSTATTPLDQLLVYEFPIRFY
                     ETRKKLGLFYNILFRPSSFRQSPWVRGFYFTGYHPGEGQSEPVPADALPADTDPTDAE
                     PVAIVNQRKLGTRYFGEEFFTEVLLADKDLAAYFQPQPTLPRYAKPAMASAMVLTLVL
                     FLAGLARSYRLNGDYVAEVEARSVRVTQLQPLQLAGDSEAASKNFQLTQLEALETLRQ
                     SLMTADAHDQTGRPWLMQSGLYVGDDIASAGRRVYFDALQQAFCRPVFLQLESDLRAF
                     AADSTPNTDEASLSQHYDRLKTYLMLSQPARAKGTFLYYQLSPYWKGVVPAEGQPLAL
                     KQLEFYALHATDQESPHLKGDELLIRQVRQRLLAYPAVSRFYKQFISEIDARVPAVTL
                     ETMLAGRGAGVLQGDYSVPGSFTLEGYRDYAADGLDAAALELAKSDWVMGETGTNLRG
                     ASIDVTRLREMYFREYTAHWQRFLKDTQVRPFKSREEAIEILTILAEPESPLVVLLTE
                     VSRQVQPTNIGSGGLIQWVTSKVSTQKKPVTTEVEREFAPLLLFLNTSPGNDGTPVSQ
                     YRVALRSILDTLQITSNDQLNQASRTLLTGKDDLGLLKAEAGVTKVTETFRTDSTRIL
                     AGLLYQPLGNLRALLYGTNGQELSTLWTQQLYPQAIRLKTGYPFTNDGGAPLTEVARF
                     LNPQDGLLASVLRERLAGSFEDVNGEWKLKPVGAVRVTPDFVGFLNQSRRLQETLFSH
                     GGRTPEIAYELTLQPSGTGKIKLEVDGVTLESQAGTPQSGKLTWPAKSGSTTGARLSV
                     TIPGGATQEMAFPGEWGMFQLIENGKPAPAGPNQFSLTWNIQSVTVTAILRSPTAVNP
                     FDRQLFASFQPPPELFAR"
     gene            3789..6488
                     /locus_tag="PKZ53_01120"
     CDS             3789..6488
                     /locus_tag="PKZ53_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ39062.1"
                     /translation="MPQAKRFLPHTHPVVWLMTICLIFYFGFGLIFSFWTRFLWTNPG
                     FTTRTDLATGIITINYIEKDSPADTVGLKVGDAIVKPDGQPFTSRSEYAQLAKKAFRT
                     NTFELTIKRENQPLQPVSIQLPPPDWQKYSFYRFYFFFVLFFIPLMCFSIGLIILWKR
                     WEDRVARMGGLFFISIGFLFGNEDSYSYLLIPAWVSTFFMVIRSFGTLSGYLCYSFFT
                     EFPVQTPLAERTRLWKRLYLAILLTILPISILSDALINYSFKLTNILRESTGDIPIKI
                     EQFLWMIGFLGLLGLFHPKLRGQSEAGQHAKFKVLMFGTVVGCGPFFSLILLDLIRFG
                     RISFNYPPLLSAIVLALYMVFPLSFGYTVVRHRVLGVQQIVRNSLQYAFVSRAYLLVE
                     ISLLYVIWKATQGIVSNVYATTLAGNPPELVSKSIFYLIGFSGLGLMWKVNPRLQTMI
                     DRRFFRSSYQAQQVLTELSQTVRQMTRAEDVLQRVAEQTKAALFVDRIGFLVHAPFLN
                     FQSEPLDRSRFYSLICRFDESPHCYFDHSKNLPSSSFVINKLKEEDLPLDLYFDEPDE
                     WMEQLIDSSRNQSSEQIKEYDLLRELDSNLLVPIATNNLFLGFLSLGPKRSEEPYTKE
                     DKNLLLAVAEATAFRLENAQLIKRVTQEATLRKELEIARTMQQSLLPAKDPILPGVEV
                     AGYSMAANDVGGDYYDYFLIDEGQLAIAVGDVTGHGVSSGLLMACAKGGVLTLVSVDP
                     APKAVMFGLNNLICTTGGKRNLMSFIYAVFDNQQHQLELTNAGHPYPYLYKAASGQVE
                     PLEISAYPLGVKRNTPYQTLTVRLQPGDTLVFYTDGIVEAQNLDGDLYGYQRLEETIA
                     AYGERSALELKDAILESAKSFAGGQPFEDDITLVVLRVQPGEH"
BASE COUNT         1593 a         1724 c         1697 g         1753 t
ORIGIN      
        1 cttcattctt cattcttcat tcttcattct tcattcttca ttcttcattc ttcaaaaggt
       61 gtacttcatg aactggcaat cactcccttt taaatcctgg ttccacctgc tcggcttgat
      121 tgtgttatat ggattggcct gtctggtcgc ctggttggtg gcgccctggc ttgggttttc
      181 tttcgttggg caacttggac tgattgggct gatattattt acctggctgt tgaccctggt
      241 gtttgaaaaa gttcgtcgaa tgtactggcc agcggaaact gatcaaactc aacaacttca
      301 accaggcaac gagagtatgg gtgatgggcc agccaatctt cctggtcaag gtgaagcctt
      361 tgcaatgttg actgactggg ttgagacgac ctgtggtcga aaaacccagg atcgttttcg
      421 acagccctgg tttggggtca tcggtccgcc cgccagcggc aaatcagctt tgcttttaac
      481 ctcacaactt gattttcagt tgcttccaca ccagcgcccc gccgatctct acacaattgc
      541 cccaaccaca atcggggaat gttatgtctc tgaatcagcc gtctatctgg atcttcccgg
      601 tcatctggtg gcagataccg cccagaatgc ggcctggaaa catttgtgcc agtggttggg
      661 tgagtatcgt gacactcgcc cggttgatgg actggtcatt gtggccgatg ctcatcggct
      721 gtgttcactt gccgaatcgg atattgaaca ggaagccaaa attttgcggg cccgcctcga
      781 tgagtgcctg cagtggtggg gtcaccagtt ccccctgtat ctggtatttt cacattcgga
      841 taaactggct gggtttgctg cattttttga ggaatatgac cgcggtgaac gcagccaggt
      901 ctggggggca accatcccgc ttgaacaaca aagcaccggc tatgcactgt ttgatactga
      961 atttgggtat ttgtatgaag cgttgatgcg tcggcgattg ttgcggttaa gtacggcaac
     1021 cacgcctctg gatcagcttc tggtttatga attcccaatc cgattttatg aaacccggaa
     1081 aaaactgggc ctgttttata atattctctt tcgcccaagc agtttccggc agtcgccctg
     1141 ggttcgcggc ttctatttta ctgggtatca ccctggcgag ggtcagtcag agccagtgcc
     1201 cgccgatgca cttccggcgg atacagaccc aactgatgcg gaaccggtgg caatcgtcaa
     1261 ccagcggaaa ctggggactc gatattttgg cgaagagttt ttcacggaag ttctactggc
     1321 ggataaagat ttggcggctt attttcagcc tcaacccacg ttgcctcgct atgcgaaacc
     1381 agccatggcg agtgccatgg tgctgacact ggtgctcttt ttggccggac tggccaggtc
     1441 gtatcggcta aacggcgatt atgtggctga ggtcgaggcc cggtcagttc gggtcaccca
     1501 attacaacca cttcaactgg ctggcgattc ggaagcggct tcgaaaaatt ttcaattgac
     1561 acagttggaa gcactggaaa cgctcaggca aagtctgatg acagcagatg cccatgacca
     1621 gactggccgg ccctggctga tgcaaagtgg cctgtacgtg ggtgacgaca ttgcatcagc
     1681 cgggcgtcgt gtgtatttcg atgcgctcca gcaagcgttt tgtcgcccgg tgtttctgca
     1741 acttgaatca gatttgcgcg cctttgcggc agactccaca ccaaacacgg atgaagcctc
     1801 cctcagccaa cactatgacc ggttaaaaac ctatttgatg ctctctcaac cagcgcgggc
     1861 caaaggaacc ttcctgtact atcaactgtc accctactgg aagggagttg ttccggctga
     1921 aggtcagcca ctggcactca aacagcttga gttttacgcc ctccacgcca ctgaccagga
     1981 atctccgcat ttaaaaggag atgaattact tatccgccag gtccgtcagc ggttgctggc
     2041 ctacccggca gtgagccggt tttacaaaca atttatttct gaaattgatg ccagagttcc
     2101 ggcggtcacg cttgaaacaa tgctagctgg gcgtggcgcc ggtgtgctgc agggtgatta
     2161 cagcgttcca gggagtttta cgcttgaagg ataccgcgat tatgccgctg atggtctcga
     2221 tgcagccgca ttggaactgg cgaaaagtga ttgggtgatg ggcgaaaccg ggaccaacct
     2281 ccgaggtgcc agcattgatg tcacccgatt gcgggaaatg tatttccgcg aatacactgc
     2341 ccactggcaa cgctttttaa aagataccca ggtcagacca ttcaagagcc gtgaagaagc
     2401 aatcgagatc ctgacaatcc tggcggaacc ggaatctccg ctcgtggtgc ttctgaccga
     2461 ggtcagccgg caggtacaac ccacaaatat cggcagtgga gggttgatcc aatgggttac
     2521 aagcaaggta tcaacccaga agaaacctgt cacaactgaa gttgagcgcg aatttgcccc
     2581 attgctgctg tttttaaaca ccagtcctgg gaatgatggc acacctgttt cccaatatcg
     2641 ggtggcgcta cgctccatcc ttgatacctt gcagatcaca tcgaatgatc agttgaatca
     2701 ggcttcccga accctgttga ctggaaaaga tgatctggga ttgctcaaag ctgaggcagg
     2761 tgtgacaaaa gtgactgaga cgtttcgaac tgattcaacc agaatcctgg ctgggttgct
     2821 ctatcaacca cttggaaacc tccgggcgct cctctatggc accaacgggc aggaactgtc
     2881 aacgttgtgg acgcaacaac tgtatccaca agccatcagg ctcaaaaccg ggtatccgtt
     2941 tacaaatgac ggaggcgctc cactgactga agtggcccga tttctcaacc cacaggatgg
     3001 gttactcgct tctgtgctac gggagcggct ggctggatct tttgaagacg tgaatggtga
     3061 atggaaactc aaaccagtgg gagccgtgcg agtcacgcca gactttgttg gatttctcaa
     3121 tcagagccgg cgactgcagg aaactctgtt ttcacatggc ggacggacac ctgaaattgc
     3181 ctacgaactc acgctccagc cttccggaac cggtaaaatc aaacttgagg ttgatggcgt
     3241 cacgcttgaa tcccaggccg gtacacctca atccggaaaa ctgacctggc cggccaaaag
     3301 tggaagtacc accggcgccc gcctgagtgt gaccatccct ggtggtgcca cacaagaaat
     3361 ggcatttcct ggagaatggg gtatgttcca gctcatcgag aacgggaagc cagcaccagc
     3421 cggacccaat cagttctcgc tcacctggaa tattcagtca gtcacagtca cagccatcct
     3481 gcggtccccc acggcggtca atccatttga tcgccagctt tttgccagct tccagccgcc
     3541 accagagttg tttgcgcggt aagaaggaac ggcagaaaaa atagtgtgga aggcggtgtc
     3601 aaaccgccgc cctccaagac agttcagacc caaaagcgaa cccttcagca gaaccataag
     3661 gacaaacagg gcgggtttcg tgtattttga gaaccgtttt tcacgattct ttcaaactcg
     3721 aaattctctg cagcccaggg tgcttgaaac ttttcttccc tgctggttac caacccgcca
     3781 gttttcctat gccacaagcc aaacgttttc ttccacatac tcacccagtt gtctggctga
     3841 tgacaatttg cctgatcttt tattttggat ttggcctgat tttctctttt tggacccgct
     3901 ttctatggac caaccctgga ttcaccaccc ggacagatct ggccaccggg atcatcacga
     3961 tcaattacat tgaaaaagac agcccggctg acaccgttgg gctcaaggtt ggggatgcaa
     4021 ttgtgaaacc ggacggtcag ccatttacca gccggtctga atatgcacaa cttgcgaaaa
     4081 aagcatttcg caccaacaca tttgagttga ccatcaaacg agagaaccag ccgctgcaac
     4141 ccgtttccat ccagttacct ccgccagact ggcaaaaata ttcattttat cggttctatt
     4201 ttttctttgt gctgtttttt attccactca tgtgtttttc cattgggttg attatccttt
     4261 ggaaacgatg ggaagatcgt gtagcccgga tgggtgggct atttttcatc tcaattggat
     4321 ttttgtttgg aaatgaagac agctattcct acctcctgat tccagcctgg gtgagcactt
     4381 ttttcatggt gattcggagt tttgggaccc tttccggcta cctgtgttac agctttttca
     4441 ctgaatttcc ggttcaaact ccgcttgctg agcgaaccag gctttggaaa cggctctatc
     4501 tggctatcct gctgaccatt cttccaattt cgattctgag cgatgcactc atcaactatt
     4561 cgttcaaatt gacaaatatc cttcgtgaaa gtaccggtga tattccgatc aagattgagc
     4621 agtttttgtg gatgatcggg tttctgggat tattgggttt atttcatcca aaactccgag
     4681 gacagtcaga ggcaggtcaa catgccaaat ttaaggtctt gatgtttggc acggtggttg
     4741 gctgcgggcc atttttctca ttgatattat tggacttaat tcgatttggt cggatttcct
     4801 ttaactaccc gccactactt tcggccattg tactggcgct ctatatggtg tttccgctct
     4861 cttttggcta caccgtggtg cgccatcggg tgttgggtgt ccagcagatt gtccgcaata
     4921 gcctgcaata tgcgtttgtt tcacgggcgt atctgctggt tgaaatttcg ctgctctatg
     4981 tcatctggaa ggccacccag ggcattgtca gcaacgtcta tgccaccaca ctggcaggta
     5041 acccacctga actggtttcc aaaagtattt tctatctgat tggattttca ggacttggat
     5101 taatgtggaa ggtcaatccg cgattgcaga cgatgattga ccgtcggttt ttccggagtt
     5161 catatcaggc gcaacaggtt ttgaccgagc tgagtcagac cgtccggcaa atgacccgcg
     5221 ccgaagatgt actccaacga gtggccgaac agaccaaagc cgcgctcttt gtggatcgga
     5281 tcggcttcct ggtgcatgcg ccgttcctga actttcagag tgaacccctt gatcgttccc
     5341 ggttttattc gctgatctgt cggtttgacg aatcacccca ttgttacttt gatcacagca
     5401 aaaatctgcc ttcaagctct tttgtaatca acaagttgaa ggaagaagac ctgcccctgg
     5461 atttgtattt tgatgaaccc gacgaatgga tggagcaatt gattgactcg tcgcgcaacc
     5521 agtcatctga acaaatcaaa gaatatgacc tgctcagaga actcgattcc aacctgctgg
     5581 tgccgattgc gaccaacaat ttatttttag ggtttttaag tctgggacca aaaaggtcgg
     5641 aggaacccta caccaaagaa gataaaaatt tgcttctggc ggtggctgaa gcaacggcct
     5701 tccggcttga aaatgcgcaa ttgattaagc gggtcacgca ggaagcaacg ctacgcaagg
     5761 aactggaaat tgcccgcacc atgcaacaaa gcctgctccc ggccaaagat ccaattttgc
     5821 caggggttga ggtggccgga tattcaatgg ctgccaatga tgtaggtggt gattattatg
     5881 attattttct cattgacgag ggacaactgg cgattgcggt tggagatgtg acgggacatg
     5941 gggtctcttc agggttactg atggcctgtg ccaaaggtgg cgtgctgacg ctggtaagcg
     6001 ttgacccggc gccaaaagcc gtcatgtttg gactcaacaa tttgatctgt accacgggcg
     6061 ggaaacgcaa cctgatgtcc tttatctatg cggtttttga caatcagcag caccagctcg
     6121 aattgaccaa tgccgggcat ccctacccat atctctataa agctgccagc gggcaggtgg
     6181 aaccccttga aatttctgcc tatccgcttg gtgtgaaacg caatacccca taccagacgc
     6241 tgacagtccg actccaacca ggtgacacgc ttgtctttta taccgacggc atcgttgaag
     6301 ctcagaacct cgatggtgac ctgtatggat atcaacggct ggaagaaacg attgccgctt
     6361 atggcgaacg ctcggccctg gaactcaaag acgccatcct tgaatccgcc aaatcctttg
     6421 ctggcggcca gccatttgaa gacgacatca cactggtcgt gctccgcgtc cagcctggtg
     6481 agcattgacc agacaacagg accataagga tcaaaaaaag agacctgagc tacttgtatg
     6541 gctctggttg agagaccgcc acgaaatttc ggaggcaacc ccgccacgaa atttcgtggc
     6601 tttttgcgtt ccacgaaatt tcggagcgca ggtgtgcctg tcaggtaagg tcaatttgaa
     6661 ccgaaaaacc ttttttccct tctggatcaa tcacttgcaa aaaacagact cacttttcag
     6721 agttttggta cgcaggttgt tttaagagag accagaaaga aacaacg
//
LOCUS       DAOQDO010000036         6727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6649_length_6727_cov_2.985612, whole genome shotgun sequence.
ACCESSION   DAOQDO010000036 DAOQDO010000000
VERSION     DAOQDO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6649_length_6727_cov_2.985612"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKZ53_01125"
     CDS             complement(<1..416)
                     /locus_tag="PKZ53_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNJ39063.1"
                     /translation="MKKHWISGWILGLIVWGTMACSTPPPPPSPPPAPPAQAPVSKQK
                     LEATCAVCGMPVVEAFQNRFEGSLATEPPVHLCSGICAASYVQRNPTLEPHLKVIDFQ
                     TRALVPAAQAFYLTGSALTIMGAMPPTVAAFSDRKAA"
     gene            909..4205
                     /locus_tag="PKZ53_01130"
     CDS             909..4205
                     /locus_tag="PKZ53_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ39064.1"
                     /translation="MTSRLETAETAFLKRPKKIPQCMLTLPEPQPSANPAFAQPSAAQ
                     FRLLVDSISSYAIFMLDPAGTAVPTDHGAEQSYGYPPEEMIGHHVSRLYLVQDVAGGI
                     PQQMMQEALTLGCYEAATHQLQGKGGRPHWATITLTALFDTSHHHIGFAGVTHACPEP
                     RHPEPTLEAALEVCEARCRTLFEYAQVGILYADTQSYYLDANESICRMLGYSRDELIG
                     LHASDIVVQSEVHHIDEALDQITNQADHHREWQFRRKDGSIFSADVVATVMPDGTLLG
                     TIQDLSDTKRAHDYHQHLAAIVESSHDAILSTDLKSTITSWNAGAEAIFGYPASEMIG
                     TSITRLFPDDRWPEENVILESLQRDERVAHFETLRRTRDGRLIDVSVAAAPIKDPDGR
                     VSGTSRILRDITMQKEREREIARLSRLYAALSQINQAIVWTATREELFQKICQVLVEH
                     GGFCLAWIGWHLPETHQLVPVAECGDQTQYLKTITVYVDDRPEGHGPSGTAFRTGQPY
                     ICNDALTDPATIPWRPALEHRGFQASAALPIRVNGAVSGTLSVYAGERNFFHDREIGL
                     LEEAVTDISFALDNFEREAARRLAEQTVRNEKQFSDMMIESMPGILYFYDSTGQFLRW
                     NRNFETVSGYSGEEIAQMHPRDFFSDAEKPDLEERIAEVFATGESFIEASFVAKDGTT
                     TPYFFTGRRLVYEGTPCLVGVGIDISDRKRAEARLAESEQKYRELVEHASSIILRWNA
                     DGYITFLNTFGRQFFGYSADEIIGRHVIGTIVPASESSGRDLTQLLEVIRTTPEAFEQ
                     NINENIRRNGERVWIAWTNRIVRDAQGRVVEFLSIGTDITDRMRIEAEQEKRHRAEAA
                     DRIKSAFLATMSHELRTPLNSIIGFTGILLQGLAGPLNPEQRKQLDMVRTSARHLLAL
                     VNDVLDISKIEAGQLEVGRSPFDISRSITKVITLVTPQAEAKGLKLNVHLAPGLGEAV
                     SDERRFEQILLNLLSNAVKFTEHGKITVTAELATDFTPPGAVSNQPAIRLRVADTGMG
                     IKPEDLPNLFQPFRQIDSGLSRRHDGTGLGLAICQRLATLMGGVISADSEWGTGSTFC
                     VTLPLQGQVK"
     gene            4205..4594
                     /locus_tag="PKZ53_01135"
     CDS             4205..4594
                     /locus_tag="PKZ53_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39065.1"
                     /translation="MKARILLIEDNAQNRYLTTFMLEQHGHEIFQAETGPQGLELAAK
                     LRPDLILLDIQLPGMDGHAVAHTLKNDPNLKSIPIIAVTSYAMIGDREKCLAAGAEGY
                     IEKPINPESFVAEVERFLLPPYKESHS"
     gene            4591..6657
                     /locus_tag="PKZ53_01140"
     CDS             4591..6657
                     /locus_tag="PKZ53_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39066.1"
                     /translation="MTHVLIVDDNAGNLYLLRMLLQGHGYSVDEARHGAEALVKARQQ
                     PPDLIISDLLMPVMDGYTLLRQWKADDRLKRIPFVVYTATYTEPRDERLALDLGATAF
                     IVKPTEPEPFMATIEEVLATASQEDPPSVAERVDEGPVLLEEYNEVLVRKLEKKAFQL
                     EASNRELLEEIAERKQAEEALRLRDRAIQEVSQGILITDPTQPDNPIIYASAGVERIT
                     GYRSDEVIGWNPRLFQGTDTDPDTLRTIREAITAGRGCSVEILNYRKDGTPFWNALSL
                     NPVHDEHGRLVNFVGVQNDITERKRLEDQLRQSQKMEAIGQLAGGIAHDFNNLLTIIT
                     GYSALLLKLPDTDHTVRKSLNAINEAGKRAATLTRQLLGFGRKTILQPKILDLNSVVT
                     ETGELLCRLIGEDILFTTVLAPDLSPVKVDPSQLDQILMNLAVNARDAMPTGGRLTIE
                     TTNVVLGDDYATTHMHCKAGPYVMLAMTDTGCGMTPDVLARIFEPFFTTKEVGKGTGL
                     GLAMVFGIVQQSGGTINVYSEPGHGTTFKIYFPAAIGHPTSENDPGANVQLSGTETIL
                     LVEDDENVCELAVTSLKMYGYKVLTATDGKEALQIIQSVSGLVDLVLTDVVMPALSGP
                     DLARMLQIRYPHLKVLFMSGYTDDAIVRHGLLEAGVAFIQKPYTPLGLVQKVRQVLDE
                     NTKEAS"
BASE COUNT         1652 a         1840 c         1771 g         1464 t
ORIGIN      
        1 gccgctttgc gatcactaaa agcagcaacg gtgggaggca tcgcccccat aatcgtcaga
       61 gctgatcccg taagataaaa agcttgcgcg gctggaacca gcgcacgcgt ttggaagtca
      121 atcactttca ggtgtggttc gagtgtcggg tttctttgca catatgaggc tgcacaaatc
      181 cccgaacaca aatgaactgg gggctctgtc gccaggctcc cttcaaaccg attctggaaa
      241 gcttcgacaa ctggcatgcc acacaccgca caggttgcct caagcttttg ttttgatact
      301 ggtgcctgag cgggtggcgc cggtggtggt gacggcggcg gcggtggggt gctacaggcc
      361 atagttcccc agacaatcaa tccaagaatc cagcctgaaa tccaatgttt tttcataatt
      421 acaccatttt gcaatgaagg tatcgtatta cgggaaggct aaattattga aaaaattgta
      481 tttccccgca accctgaacc cgataccctg aaccctaatt ggtattacca gtttttcgtg
      541 agtcgttcac ccggcttctt tcagtcaatt tcctggtggg tgagccgtgg attttaataa
      601 atgaatcagt gcggcaatct ctgattgtgg tgatgaagaa tcaacaaacc gtgaattcca
      661 acccatattc catctaaaat tgtatttcaa ctgaggatcg gcactttttc gcctgaactc
      721 cggaaaaaat ccagccagtt cccccaacgt catgcagtac ctggtcagaa agtttcttga
      781 tggagcgtac actattggca gtaattcctt tatagttacc aaaaagataa ccagcccttc
      841 tagacctttg ccccgaggtc agacatcgaa catacaggct atgctcgact gattgaaact
      901 gtgggcatgt gacttcccgc cttgaaacgg ctgaaacggc ttttctcaaa cgccccaaga
      961 agattcctca atgtatgctg acccttcctg aaccccaacc ctctgccaac cctgcttttg
     1021 cacagccatc tgccgcgcag ttccggttgc tggttgactc aatcagcagc tatgccattt
     1081 ttatgcttga tccagccggc accgcggttc caactgatca cggtgccgag caaagctacg
     1141 gatacccacc tgaagaaatg atcggccacc acgtttcgcg gttatacctg gttcaagacg
     1201 tggctggcgg tattcctcag cagatgatgc aggaagctct cactcttgga tgttacgaag
     1261 ccgccaccca tcaactccag ggcaagggtg gaaggccaca ctgggccacg atcacactga
     1321 ccgcgctttt tgataccagc caccatcaca ttggttttgc cggagtcacc cacgcctgcc
     1381 ccgagccacg acaccctgag ccgacacttg aggccgccct ggaggtgtgt gaggcacggt
     1441 gccgcacact gtttgagtat gcccaggtcg gcatcctcta tgctgatacc caaagctatt
     1501 atcttgatgc gaatgagagt atctgccgga tgcttgggta ttcacgtgac gagctgatcg
     1561 ggttgcacgc ctccgatatt gtcgttcagt cagaagtcca ccatattgac gaggcattag
     1621 accagatcac caatcaggct gaccatcacc gggaatggca atttcggcgc aaagatggtt
     1681 cgattttttc ggcggatgtg gtggcaacgg ttatgccaga cggcacattg ctgggcacaa
     1741 tccaggatct ctctgacacc aaacgggctc acgactatca ccagcatctg gcggcgattg
     1801 ttgaatcgtc gcacgatgct attttgagca ccgatctgaa gagcaccatc accagttgga
     1861 atgccggcgc tgaggcaatt tttggatacc cagccagcga gatgattgga acttcgatta
     1921 cccggctgtt tccagacgac aggtggccag aggaaaatgt catactggaa agtctgcaac
     1981 gggacgagcg cgtggcacac tttgagacgt tgcgtcggac cagggatggg cggctgattg
     2041 atgtttcagt tgcggcagcc ccaatcaaag acccagatgg tcgcgtaagt ggaacctcaa
     2101 ggatcttgcg tgacatcacg atgcaaaaag agcgcgagcg cgagattgcc cgtctctcaa
     2161 ggctctatgc ggcattgagt cagatcaacc aggcgattgt ctggacggca acccgtgaag
     2221 agctgtttca gaaaatttgc caggtcctgg ttgagcatgg cggattctgt ctggcctgga
     2281 tcggctggca tctcccagaa acacaccaac tggtgccggt ggctgagtgt ggcgaccaga
     2341 cgcaatacct caaaaccatc acggtgtatg tcgatgatcg accagaaggc catgggccgt
     2401 cgggcaccgc ctttcgcacg ggtcagccct atatttgcaa tgacgcgttg accgacccgg
     2461 ccacaatccc ctggcgtccg gcgcttgaac accgggggtt tcaggcttcg gcagcacttc
     2521 cgattcgggt gaatggagca gtatctggca cgctcagtgt ctatgccggg gagcgaaatt
     2581 tcttccatga ccgagagatc gggctccttg aagaagccgt caccgatatt tcttttgccc
     2641 tcgacaactt tgaacgtgag gccgcacgcc gcctggcaga gcaaacagta cgcaacgaaa
     2701 agcagttctc agatatgatg atcgagagca tgcccggcat cctctatttc tatgattcaa
     2761 ccggacaatt tttgcgctgg aatcgcaact tcgagactgt ttccggctac tctggcgaag
     2821 aaattgccca gatgcaccca cgcgatttct tctcagacgc agaaaaacca gacctggagg
     2881 aacgaatcgc agaagtattc gcaaccggcg agtcatttat tgaagcgtcc tttgtcgcca
     2941 aagatggcac caccacaccc tactttttca ccggacggcg cctggtgtat gaagggacac
     3001 cgtgcctggt gggggttgga attgacatct ctgatcgcaa acgcgccgaa gctcgcctgg
     3061 ccgagagtga gcaaaagtat cgcgagctgg tcgaacacgc cagcagcatc atcctgcgct
     3121 ggaatgcaga tggctacatc acatttctca acacgtttgg ccggcagttc tttggatatt
     3181 ctgccgacga gatcatcggt cgccatgtca ttggcacgat tgtcccagcc agtgaaagca
     3241 gtggtcgtga cctcacgcaa ctgctggaag tcatccgcac caccccagag gcatttgagc
     3301 aaaacatcaa tgaaaacatc cgccgcaatg gcgaacgcgt ctggattgcc tggaccaacc
     3361 gcatcgtgcg ggatgcacag ggccgtgtgg ttgagtttct gagcatcggc accgacatta
     3421 cagatcggat gcgaatcgaa gccgaacagg aaaaacgcca ccgggctgag gccgcagatc
     3481 gcatcaaatc agcctttctg gcaaccatgt cgcacgagtt gcgtacgccg ctcaattcga
     3541 ttattggatt caccgggatt ctcttacagg gactggccgg gcccctgaac ccagaacagc
     3601 gcaagcaact tgatatggta cggaccagcg cccgccacct gctggccctg gtcaatgatg
     3661 tgctcgacat ttccaagatc gaagccgggc aacttgaagt tggtcggtca ccatttgaca
     3721 tctcccggtc aattaccaaa gtcatcacgc tggtcacacc acaggccgag gccaaagggc
     3781 tcaaactcaa tgtgcatctg gcaccgggac tgggcgaggc cgtgagcgac gagcgccgtt
     3841 ttgagcaaat tctgcttaat ttattgagca atgccgtgaa atttactgaa catggcaaaa
     3901 ttacagtcac cgctgaactg gcgaccgatt ttacgccgcc aggggcggta tcaaatcagc
     3961 cagccatccg gctacgtgtg gctgacaccg gtatgggcat caaaccagag gacttgccaa
     4021 acctgtttca accttttcgc cagattgact caggtttgtc acgacggcac gacggtacag
     4081 ggttgggatt ggctatttgt cagcgtctgg ccaccttaat gggcggcgtg atcagcgccg
     4141 acagtgaatg gggaacgggc agcacttttt gtgtcacgct gccattgcaa ggacaggtga
     4201 aatgatgaaa gccagaattc tactgattga agacaatgcg caaaaccgct atctgaccac
     4261 atttatgctc gaacagcacg gccatgagat tttccaggcg gaaaccggtc cgcaaggatt
     4321 ggaactggca gccaaactcc gcccggatct gatcttgctc gacattcagt tgccaggtat
     4381 ggatggccac gccgtagcgc acaccctaaa aaatgaccca aacttaaaat ccattccaat
     4441 catcgcggtc acgtcatacg ccatgattgg tgaccgggaa aaatgcctgg ctgccggcgc
     4501 cgaaggctat attgaaaaac caatcaaccc ggagtcgttt gtggctgaag tcgaacgctt
     4561 cctccttcca ccatacaagg aatcccactc atgacccacg tgctgattgt ggacgataat
     4621 gcgggaaatc tgtatctgct gcggatgttg ttgcagggtc acggctacag cgtagatgaa
     4681 gcccggcatg gtgctgaggc actggtgaaa gcccggcaac agccacctga tctgatcatc
     4741 tctgacctgc tgatgccggt catggatggc tacacactct tgcgacaatg gaaagctgat
     4801 gaccgactca aacgtattcc ctttgtcgtt tacaccgcaa cgtacaccga accaagggac
     4861 gaacggctgg cgctggatct gggtgccact gccttcattg tcaaaccgac ggaaccagaa
     4921 ccctttatgg caaccattga ggaagttctg gccacagcca gccaggaaga cccgccctca
     4981 gtggcagaac gagtagatga aggaccggtg ctccttgaag aatacaacga ggtgttggtc
     5041 cgcaaactgg agaagaaagc ctttcagttg gaagcctcca accgtgagtt gctggaagag
     5101 attgccgaac gcaaacaggc agaggaagcc ttgcggcttc gagaccgggc cattcaggag
     5161 gtctcgcaag gaattctcat taccgatcca acccagccgg ataatccgat tatttatgcc
     5221 agcgccgggg ttgagcgaat tacaggctat cggtcagacg aggtgattgg ctggaaccca
     5281 cgcctgtttc aaggtacgga taccgatcca gacaccctcc gtacgatccg cgaagcgatt
     5341 accgccgggc gcggctgttc ggtcgaaatt ttgaactatc gcaaagatgg aacgccattc
     5401 tggaacgccc tgtcgttaaa ccccgtgcat gatgaacatg gaaggctggt gaatttcgtt
     5461 ggtgtccaaa atgacatcac cgagcgcaaa aggctggaag accagctccg gcagtcccaa
     5521 aaaatggagg cgattggtca actggctggc ggcattgccc acgacttcaa caacctgctc
     5581 acgattatca ccggctatag cgcactgttg ctcaagctgc ctgacaccga ccacaccgtc
     5641 cgcaagtccc tcaacgccat caacgaagct ggaaaacggg cagcgaccct gacccggcag
     5701 ctcctgggtt ttggccgcaa gaccattttg caacccaaaa tccttgatct caatagcgtt
     5761 gtcactgaaa ccggggaatt actgtgccgc ctgattggcg aagatattct ctttacgacc
     5821 gtgctggccc cagacctcag cccggtcaaa gttgatccga gccaactgga tcaaattctg
     5881 atgaatctgg cagtcaacgc ccgtgacgca atgcccacag gtggcaggct gacgattgaa
     5941 accaccaatg ttgtgctggg tgatgactat gccacgaccc atatgcactg caaggcgggg
     6001 ccgtatgtga tgctggccat gactgatacc ggttgtggga tgacgcctga tgtcctggct
     6061 cggatctttg agccgttttt taccaccaag gaagtcggca aagggactgg cctgggactg
     6121 gccatggttt tcggcattgt ccagcaaagc ggcggcacga tcaatgtcta tagcgagcca
     6181 gggcacggca ccacattcaa aatatacttc cctgccgcta tcgggcaccc aaccagcgaa
     6241 aacgatccag gggcaaacgt tcaactctcg ggcactgaaa ccattctgct ggttgaagac
     6301 gatgaaaacg tgtgcgagct agccgtgacc agcctgaaaa tgtacggcta caaggtgctc
     6361 acggcaactg atggaaaaga agcgttacag attatccagt ctgtcagcgg attggttgat
     6421 ctggtcctga cggacgtggt aatgcctgct ctgagcgggc ccgaccttgc cagaatgtta
     6481 cagatccggt acccacacct caaagtcctg tttatgagtg gctatactga cgacgccatc
     6541 gtccgccatg gcttgctcga agccggtgtc gcttttattc aaaaacccta taccccgctg
     6601 ggactggtcc aaaaagtgcg ccaggttctg gatgaaaata ccaaagaagc aagctgaggg
     6661 cctgcagctt atgttgaacc gaacgacgag cgcaatctcg gcctttcaag gccgagtcaa
     6721 gcgagtc
//
LOCUS       DAOQDO010000037         6653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6779_length_6653_cov_2.820400, whole genome shotgun sequence.
ACCESSION   DAOQDO010000037 DAOQDO010000000
VERSION     DAOQDO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6779_length_6653_cov_2.820400"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..661
                     /locus_tag="PKZ53_01145"
     CDS             71..661
                     /locus_tag="PKZ53_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39067.1"
                     /translation="MSISPTAAPPSARKHPVLAELFRKAEGKHFTDEELASIEKLYPQ
                     LKPSIAAAKEVRSKDVAIIGRVVKEVFSQYPYEKFHDFANPKCIRDVRYVVIYAVQAL
                     LAQDPHWFEDKLLIWFKTILQAFDFPDRSVQSAGALFADPILEEKLKQLPKKTKSIFH
                     CYYRLKQEMKKDLKPEHFELLASYFDQATSTLSEKY"
     gene            677..1324
                     /locus_tag="PKZ53_01150"
     CDS             677..1324
                     /locus_tag="PKZ53_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39068.1"
                     /translation="MMTSEQYIDQMDLSVFFDETQLAFDLREGVLWNQAKTRVCLLST
                     DLILGVYKALVDEAGPAWSMILKNCGQVWGGRVVKRLDRECQLLLGQQLGEMPLKDFN
                     HFLSQYFVFHGWGELSLDVSQATERGLVEATLRNSIFADVINDPDEMVDPMIAGMLAS
                     ILSYLSGQTLDCVQTECKTRGAAHSRFIITAPSRLKNYEVMVKSNLSHDQIAASI"
     gene            1419..3221
                     /locus_tag="PKZ53_01155"
     CDS             1419..3221
                     /locus_tag="PKZ53_01155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ39069.1"
                     /translation="MTVRQPLQGYEILKLIGSGSFGLVYLAQDLKNQTLCAIKEFPVS
                     PKTAQHFFRELSLLFTLDHPNIIKCLNLIYEQDRKNYLVFEYADGGSLRDVLNQKVTL
                     EVTEALEAVRHIALGLAHAHEHQIAHRDLKPENILICTENGQSVFKVSDLGISCQIAN
                     LDAQMPSGSPAYMAPEQFYDTSSFASDLYSLGVMFYEMLTGDLPFMGMPAHLFALHLR
                     EEADFDLISHVECRQLIRQLMAKQPHARPTSAFEVVQRIDQILGTFHQAPVLEPPVQS
                     GSKSVETAVSRSDFGRQHWTLRLVSTKNIPGSRNLFALAPWDTASLLISDARSTDGYN
                     IETDRFYPQMLSEPICAAFSPAPETLTTCFATPKYFYSFSTRTQTIRRLFPHRTRITA
                     MTCLSLPGEGGVRLVFTDSRRVYCTGLDGKQFWQAECKNYFLQPQLLGLSDGTLLVSS
                     SPVTPTLHGFGPDGKRKFHIELEAPVLALYQSDQDQMIHALLFGLEKQFGMQHLVLTP
                     DGIVTETEWMEGIYAVNVHLPFLSLFQSPNKVLLANENGQIFGEYQSAGEILADAWLP
                     IQNTYAVLDKVGHQSFLNLFQFVPQAGQSDEMTQ"
     gene            3350..3955
                     /locus_tag="PKZ53_01160"
     CDS             3350..3955
                     /locus_tag="PKZ53_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39070.1"
                     /translation="MKRPFSIFIIRHAVDGALLAMRRAPQSGSTLVVLTIGSPDLVAL
                     LGATPSRLSPTLADFLTETGTSRLPVITNGAQKVSILFPHSAATTGTPQAELLDRLRT
                     QADPWEQISLVLPYERLEVDLPPLGPDDQVTCFWEVRDLVTESQTQNFSYLAERIENE
                     RPRFEGLRFYTREPGNLQLLEDRKELIESTAGLQPSMLIQL"
     gene            3997..4710
                     /locus_tag="PKZ53_01165"
     CDS             3997..4710
                     /locus_tag="PKZ53_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39071.1"
                     /translation="MQPDQKRVPQSKALSDAQLSELEVLGAMWPNVIHRVSNILTRAV
                     GKALILKYQLPDLSHHPSAQTTLAGLNQALNELGDQFKELARFQQGFFQGRGEVTETD
                     LHHLMAHLQAEHPQWHISLPRLPASRLYIHPVYGLAAINGLVHETGTSTGKLSLSIEP
                     ADSAAASSPDCQLQVQIKVETTTSLNFGPRLTDHHNPNLIACIELIGQMGGTIEVVAD
                     PPHQLITLTFQGLLTPELP"
     gene            4723..5448
                     /locus_tag="PKZ53_01170"
     CDS             4723..5448
                     /locus_tag="PKZ53_01170"
                     /inference="COORDINATES: protein motif:HMM:NF023294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HNJ39072.1"
                     /translation="MNHQSPAETTALQSEFIEKAAQAAQTAPIVEGDPAASQLLAEAR
                     SLIYHWPADFAGFEARLLVLDNEHTFTGHVKARSSRACEVQLDGYEHLKWVRYQLEEL
                     IAHREAPQVSQMSSRTGVVFGDWNAIYGRKVTFLGDRMQSFYRIKDRKLTQIGRSYNG
                     LSFVINIDQHQEFEGQFVSTHYTVFYVSTATNQLAKVETFFDRYQEVNGRLLPAERRY
                     TLVEGHQGGMTTRSLLFQDVTQL"
     gene            5468..>6653
                     /locus_tag="PKZ53_01175"
     CDS             5468..>6653
                     /locus_tag="PKZ53_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39073.1"
                     /translation="MKIFDYAAGEFTPTQTQLALNKEFSDEASYLEVVVAEHQRQIKA
                     QSRPIAAGAIPILQQARPETSPVPEPELVQLIELLASSLSAGIDTPVPFGGTETAWFL
                     KVTDLNGDWNQATTKPSETKVLLWEHAIKAAIQSASQELALHHYVVFEQRAEEALQRL
                     RRLRAAVHLHLNAREIAGFASDNFIQYCRMETPEIPDLEAQELKHLLGMLNVTFVNLL
                     KTHNESEVVLNLCTFWAQHLIPRYDCSLQEWQFLVASIELGVHRVAGDWCSRELARYF
                     LILNQAVTRFAFCGVFFRNFDDVLTRINQPLGKLPFDSREYLRTALGQLLLDEGSSGS
                     VCLQQWGILTTRKPLGGVQPEVFRRFTVQAVNACRHLAERSWLDWLEEELNRAAQTLE
                     YAH"
BASE COUNT         1584 a         1788 c         1657 g         1624 t
ORIGIN      
        1 tatccgagga ttcgattgta aactggtgta cctaaattca agacccattt ttcgaggtac
       61 aaagttcaat atgagcattt ccccaaccgc agccccacct tcagcccgaa aacatcccgt
      121 tctggctgaa cttttccgca aagccgaagg caagcacttt actgatgaag agctggccag
      181 tatcgaaaaa ctctatcctc aactcaaacc atccattgcc gctgccaaag aagtccgatc
      241 caaagatgtg gccattattg gacgagttgt caaagaggtc ttttcacagt acccctatga
      301 aaaatttcat gactttgcca accctaaatg cattcgggat gtgcgctatg ttgtgattta
      361 tgccgtccag gcgttgcttg cgcaggatcc acactggttc gaagacaaac tcttgatctg
      421 gtttaagaca attttgcagg catttgactt cccggaccgg tcagtacaat cagcgggagc
      481 gttgtttgct gatccgattc tggaagaaaa actcaaacaa ctgcccaaaa agaccaagtc
      541 aatcttccat tgttattacc gattgaaaca ggaaatgaaa aaagacctca aaccagagca
      601 tttcgagtta ctcgcttcct atttcgatca ggcaacgtca acactcagtg aaaaatacta
      661 gaggagcctc ccggccatga tgaccagtga acagtatatt gaccaaatgg accttagcgt
      721 tttctttgat gaaacccagc ttgcctttga ccttcgggag ggggttttgt ggaatcaagc
      781 caaaacccgc gtctgcctgc tgtcaaccga tttgattttg ggcgtctata aagcactggt
      841 ggacgaagct ggcccggcct ggtcaatgat tttgaaaaac tgtggccagg tgtggggtgg
      901 acgcgtggtc aaacggctgg atcgtgaatg ccagcttctg ctcggccagc aacttgggga
      961 aatgccgctc aaagacttta accactttct gagccagtat tttgtctttc atggctgggg
     1021 tgaactctcg ctggatgtct cacaggctac tgaacgcggg ctggttgaag ccacgctccg
     1081 caattcgatt tttgccgacg ttatcaatga cccggatgaa atggttgatc cgatgattgc
     1141 aggcatgctg gccagtatcc tctcgtacct gagcggccaa actctggact gtgtccaaac
     1201 tgaatgcaaa accagaggtg ctgcccacag ccggtttatt atcacggccc ccagtcgtct
     1261 gaaaaattat gaggtgatgg tgaagtccaa tctgagtcac gaccagattg cggcgtctat
     1321 ttaggggtca ggggtcaggg ttttcgaatt ttcgtccttt gtgtccctta tgtccttttc
     1381 gtcctttctt gcttttccat caaaggggct ttctttcaat gaccgttcgg cagccgttac
     1441 agggctatga aattctaaaa ctgattggga gcggctcgtt tggactggtc tatttggcac
     1501 aggacctgaa aaaccagacg ctctgcgcca ttaaagaatt tccagtaagc cccaagacgg
     1561 cccagcattt ttttcgtgaa ttgtcgttgc tcttcaccct ggaccatcca aacatcatca
     1621 agtgcttaaa tttgatctat gagcaggacc gcaaaaacta cctggtcttt gaatatgcag
     1681 atggcggcag tttgcgggat gtactcaacc aaaaggtcac acttgaagtc accgaagcac
     1741 ttgaagccgt gcgccatatt gcgctgggtc tggcgcacgc tcacgaacac cagattgccc
     1801 atcgggatct caaaccagaa aacattttga tttgcactga aaatggccag tcggtgttca
     1861 aggtgtctga tttggggatt tcgtgtcaga ttgccaacct tgatgcccag atgccgagtg
     1921 gttcaccggc gtatatggcc cccgagcagt tttacgatac atcctcattt gccagtgatt
     1981 tgtactcact gggtgtgatg ttttatgaaa tgctcaccgg ggacttgccc tttatgggca
     2041 tgccggctca cctctttgcc ctgcatctgc gtgaagaagc agattttgac ctgatttcac
     2101 acgttgagtg ccggcaattg attcgtcaat tgatggccaa gcaaccccac gcccgtccga
     2161 ccagtgcgtt tgaagtcgtg cagcggattg accagatcct gggtacgttt catcaagctc
     2221 cggtcttaga gccacctgtg cagtcagggt cgaaatcagt tgaaactgcc gtgagccgct
     2281 ctgactttgg tcgccagcac tggacgctgc ggttggtgtc cacgaaaaac attcctggga
     2341 gtcggaattt gtttgcgtta gctccgtggg atacagccag tctgttgatc agtgatgccc
     2401 gcagcaccga cgggtacaac attgaaactg accggtttta tccccaaatg ttgagcgaac
     2461 ccatttgcgc cgccttttcg ccggcaccag aaactctgac aacctgtttt gccacgccga
     2521 aatacttcta cagtttttcg acccggacgc aaaccatccg ccggcttttt ccacatcgca
     2581 cccgcattac ggcgatgacc tgcctttcac ttccaggtga aggcggggtg cggctggtgt
     2641 ttaccgactc acggcgagtg tattgcaccg gactggatgg caagcagttc tggcaggccg
     2701 aatgcaaaaa ctacttttta cagccgcagt tgttggggct gagcgatggc acgctgctgg
     2761 tctcgtcaag cccggtaacc cccaccctgc acggatttgg ccctgacgga aagcgaaaat
     2821 ttcacattga actcgaagcc ccggtgctgg cgctctatca atcagaccag gatcagatga
     2881 tccatgccct gctctttgga ttggaaaaac aatttgggat gcagcatctg gtgctgacgc
     2941 ctgatgggat tgtgactgaa accgaatgga tggaaggaat ttacgctgtc aatgtccatc
     3001 tgccctttct ctcgctcttt caatctccca acaaagtact gctggcaaat gaaaacgggc
     3061 agatctttgg cgaatatcaa tcggcgggtg aaattctggc cgatgcctgg cttccgattc
     3121 aaaataccta tgcggtactc gacaaagtcg gtcaccagag ctttctcaat ctctttcagt
     3181 ttgttcccca ggcaggacaa agtgacgaaa tgacacagtg acaggatgac gaaatagggt
     3241 tcagagttca gggttttcgc cgcgtagcgg tgattgaatt gagacaggtt gtttacagcc
     3301 tgcttttcga acccggaacc cggaacccgg aacccggaac cctcaactta tgaaacgacc
     3361 tttttcaatt tttatcattc gacacgcggt tgatggtgcg ttgctggcca tgcgacgcgc
     3421 gccccaatct ggatcaacac tggtggtgct gaccatcgga agccccgatc tggttgcctt
     3481 gcttggggca acccccagtc ggttatcgcc aacactggcc gatttcctga ctgaaaccgg
     3541 tacgtcgcgc ctgccggtca tcaccaatgg ggcgcaaaag gtatccatcc tgtttccgca
     3601 ttcagcggcg accaccggga caccccaggc tgaactgctc gaccggttgc ggactcaggc
     3661 tgacccgtgg gagcaaattt cgctggtctt accttatgaa cgcctggagg ttgatctccc
     3721 gccacttggt cccgacgatc aggtgacctg tttttgggaa gttcgggacc tggtcaccga
     3781 atcccagacc cagaatttca gctatttagc ggaacgaatc gagaatgaac gtccccggtt
     3841 tgagggtcta cggttctaca cccgtgaacc cggcaacctg caattacttg aagaccgcaa
     3901 agagttgatt gaatcaaccg ccgggcttca acccagcatg ttaatccagc tttgatgttt
     3961 tctagatacc atcaccaacc tcaggggtga gacaggatgc aaccagatca gaaacgggta
     4021 ccccaatcta aagccctatc ggacgctcaa ttgtcagaac tggaagttct gggcgccatg
     4081 tggcccaacg tcatccacag ggtttcaaat attcttaccc gggctgttgg gaaagcgttg
     4141 attttaaaat accagttgcc agatctgagc catcacccgt cagcccaaac gacactggca
     4201 ggactcaacc aggcactcaa tgaactgggt gaccaattca aagaactagc ccgttttcaa
     4261 cagggatttt ttcagggacg tggagaagtt actgaaactg acctgcacca cctgatggct
     4321 cacctgcagg ccgagcaccc acagtggcac atcagcctgc ctcgacttcc agcttctcgt
     4381 ttgtacatcc atccagtcta tggactggca gctatcaatg ggctggtaca cgaaactggg
     4441 acctccacag gcaagttgtc gctgtcaatt gaaccagccg acagcgcggc tgcctcatca
     4501 ccagactgcc agcttcaggt tcagatcaag gttgaaacca cgaccagttt gaactttgga
     4561 cccagattga cagaccatca caaccctaac ctgattgcct gtattgaact tattggccag
     4621 atgggcggca ccatcgaggt ggtggctgat ccgccacatc agttgattac cctgaccttt
     4681 caaggtcttt tgacaccgga acttccttaa agatcatcac ttatgaacca tcaaagtcct
     4741 gctgaaacga ctgctctaca aagcgaattt attgaaaagg ccgctcaagc tgcccaaaca
     4801 gcgcctatcg ttgaaggcga cccggcagcc agtcagttgc tggctgaagc ccgatcattg
     4861 atttatcact ggccagccga ttttgctgga tttgaagccc ggctgctggt gcttgacaac
     4921 gaacacacct ttaccggcca cgtcaaagca cgctccagcc gtgcctgtga agtccagctt
     4981 gatggatatg agcacctcaa atgggtccgc tatcagcttg aagaactgat tgcccaccgc
     5041 gaagcgcccc aggtcagcca gatgagttcg cggactgggg ttgtgtttgg ggattggaat
     5101 gcaatttatg gccgcaaggt gacgtttttg ggagatcgga tgcagagctt ttatcgcatc
     5161 aaagaccgca agctgaccca aattggccgc agttacaacg gcctctcttt tgtcatcaac
     5221 attgatcagc accaggaatt tgaaggtcag tttgtctcca cccattacac ggttttttac
     5281 gtctcaacag ccaccaacca gcttgccaaa gttgaaacct tttttgatcg ctatcaggaa
     5341 gtcaatgggc ggctgctgcc ggctgaacgc cgctacacgc tggttgaagg ccaccaaggc
     5401 gggatgacaa cacgttccct gctgtttcag gacgtgaccc aactttgatt gtctctcaag
     5461 gtgacttatg aaaatttttg actatgccgc cggagaattt actccgactc aaacccaact
     5521 tgcactgaat aaagaatttt cggatgaggc ttcatacctt gaagtcgtcg tcgccgagca
     5581 tcagcgccag atcaaagccc agtcccgacc gattgcagcc ggggccatcc cgattttaca
     5641 gcaggcccgg cctgagacct cgccggtgcc ggaaccagag ctggtccagt tgattgaatt
     5701 gctggccagt tccctgtcag cggggattga taccccggtt ccatttggcg gcaccgaaac
     5761 agcctggttt ttgaaggtca ctgatctgaa tggcgattgg aatcaggcaa cgaccaaacc
     5821 ttcagaaacg aaagtcctgc tctgggaaca cgccatcaaa gcggccattc aatctgccag
     5881 ccaggagctg gcgctccatc actatgtggt gtttgagcaa cgtgccgaag aagccctgca
     5941 acggctgcgc cgcctcagag ccgcagtcca cctgcacctc aacgctcgtg aaattgccgg
     6001 gtttgcctct gacaacttta ttcagtattg ccggatggaa acgccagaga ttccggatct
     6061 ggaagcgcag gaactcaaac atttgctcgg gatgctcaat gtcaccttcg tcaatttgtt
     6121 gaaaacccac aatgaaagcg aagtcgtgct caacctgtgc acgttttggg cccagcacct
     6181 gattccacgc tatgattgct cattacagga gtggcaattt ctggtggctt cgattgaact
     6241 tggcgttcac cgtgttgccg gtgactggtg cagccgtgaa ctcgcccggt acttcctcat
     6301 tctcaatcaa gccgtgaccc ggtttgcctt ttgcggtgtc tttttccgaa atttcgatga
     6361 tgtgctgacc cgcatcaacc agcctctcgg gaaattaccg tttgatagcc gcgaatacct
     6421 tcgaaccgct ctgggtcaac tcctgctgga tgaaggctca agcggcagcg tctgtttgca
     6481 gcaatggggg attctcacga ctcggaaacc actcggcggc gttcagcccg aagtattccg
     6541 gcgctttacc gttcaggcag ttaatgcctg ccgccatctg gccgagcgaa gctggctcga
     6601 ctggctggaa gaagaactca accgggcggc gcaaacgctg gaatatgcgc acg
//
LOCUS       DAOQDO010000038         6609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6844_length_6609_cov_2.862679, whole genome shotgun sequence.
ACCESSION   DAOQDO010000038 DAOQDO010000000
VERSION     DAOQDO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6844_length_6609_cov_2.862679"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKZ53_01180"
     CDS             complement(<1..265)
                     /locus_tag="PKZ53_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HNJ39074.1"
                     /translation="MFLKASRPFSLLLIWLLFAPGLSLLADNSTKKLDTKEDPEKIGK
                     RNINKGSIQFYSMEKEMAIGQQLAADLDRQLKFVTDPLVTEYVN"
     gene            460..4296
                     /locus_tag="PKZ53_01185"
     CDS             460..4296
                     /locus_tag="PKZ53_01185"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HNJ39075.1"
                     /translation="MCYIRKPSMPPQNIKSYIDIQQLDQISNFREAEGERVVRAYHFT
                     SELGRLVSRNLEAIASPRTPGAIRIIAGPRGVGKSHLLMLIQTLAYKPRARALVSQVP
                     QVSNAVRRLEGSSFVVVQVSGPTRGATTFDFGIALREALRHSPGNPMEFDDTQWYLAI
                     EHGQVCELICSKLFPGMVLLVTIDDVSEIFRSGKRELITNTYRWLSLLAMKARDLPIV
                     VLLALDSDVIDPQTGPAAKLTADYQVDALSQESLRRIADQALFRKNPIQRSELQALYR
                     EALRLLPNFKWSEQEFIEMYPVHPVVLDVSPSMCQYCESFSLFGFMNAAAGRALGRRP
                     LQLISLDELFDRFEFDLRRNPVLQRGLEVYDFLASIVIPTLAFGDRLKAKLILKGILL
                     SSISGRTVNAYDLTDSLMLYEERSTDRESAYRQTFAILQQLENLAEGQLIGKEEGISR
                     QYQITIVERLDPEAKLNAAAAEIAFDDPRLCALLVTLGGICFQEWPFSADALLMANAV
                     EQRTEVTGTWRGTHRKGVVVFNQPVELAMLQPVQIERHEERLTPDAISVDAEVFEVKS
                     SVTTPSGLQEAITTEVKVTYTYQWAEECCELDWQISLLPAIYPEIPDTVPPIPSLLFW
                     KPGMLDAAGCDILKKAWVVYTRGEEVLGIEAADRLQYLEIQVRELFLKLYIEQGLLVS
                     ATGEKYSAKNFWPDGTLQKRFVDFVGVLIQTPLDKRFPLHPVFQSGLTEREVRRLVLG
                     LMGGLNPTDAAVQEYALSYAVPLHIVTEREGVYQLAVDRDEALSQPFISEVLRLVEAS
                     ASGDPIPVQVIYQTLRREPYGLLEPVQQLIMMALIAGWRIELIDVSGLRVFGAAELTQ
                     DVSFRQYGTVRRTATISYSAEVLTEWCRLLTERPDLPELTAVQARKMIREALAGWYQR
                     WADLNLAERFNSLPVEMMTTRTWQLIITCKRYFETTSNAVEAVLHERISLEMGLARIV
                     EVFSANPTIFQRARHEFQLLVEFLNWLPYYIRAKTYTLTAVQLNNEEINAHRRELTHF
                     FDHPHRLLDEDKRRRFEATFRSFQDKFTNLYATKHEDAMTKLVNLETLEEFLASRLWR
                     TFELVSQLNIANPRFLVLAQEMIKLIRESHCTLPVRDILSIQPACSCGFRVGSVTPIS
                     TMVEDLKQLVRTGVDYHRRLIFQYRPKLSQNLRTATTATGSNKDQVGALLALISSGES
                     TTAMTMTPGMVELINEFLKEETRVIPASPPAPFVLGDRLTKAELKSRLSSWLESLPDD
                     GDLAFEISESPFAGEN"
     gene            complement(4387..6075)
                     /locus_tag="PKZ53_01190"
     CDS             complement(4387..6075)
                     /locus_tag="PKZ53_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNJ39076.1"
                     /translation="MTMNHPHPFSTSRRFAYRISCFVLMFLCLASTNPLLARQEGEKK
                     KQKRKVEVEKVYQKWLDEDVRYIITKEERDAFKKLLTDEEREEFIEQFWLRRDPDPDT
                     TENEYLEEYYRRIAYANERFSSGIPGWKTDRGRIYITFGPPDSIESHPAGGPYERPYY
                     QGGGQTSTFPFEIWFYRFIDGIGSGIEIEFVDPSGSGEYRIAQSPDEKDALLFTPNGG
                     LTLYEQLGITSKRDRPYFSPGNSNSSQFVRRAQDQPFERLSLYANLQRAPQVKYRALA
                     DKLEKVDQLTEFDVLPLSLRTDYIRAGEAQGVMVFTTQVKNSDLSFKNVGGIQQATVN
                     IFARVTALSGKRAGIFEDPATIAFPESQFDSGIQQSSVYQKSVVLPPGRYKIDMVVRD
                     ITSGHTGVVHHGFEVPQYSQEKMITSSLIIADLLEPLRGRVAAGPFIVGSNKVRPNVS
                     QVFKTNQSLGVYLQVYNVQIDQTTLQPAVDVEYVITKNGQEVLRLKEDGKNGISDLSG
                     SGQQIILGRSIPLTSLEPGDYEVTVSITDRVGRQTLTPKTNFTVESASQLTSSR"
     gene            complement(6204..>6609)
                     /locus_tag="PKZ53_01195"
     CDS             complement(6204..>6609)
                     /locus_tag="PKZ53_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39077.1"
                     /translation="FAFLITVPFMIWGINTTNSTEFVIATFFISYFMGWYHGPVAAIV
                     GEIVPASLRGTAIALYMFGIHILGDTPAPAIIGYLSDRLSTTGGLRSALFLTIIANGL
                     AGLLFILVTFVLRRRAGLTDSMPVTAVATAQE"
BASE COUNT         1596 a         1579 c         1734 g         1700 t
ORIGIN      
        1 ggttgacata ttcggttacc agtggatcag tgacaaattt gagctggcgg tccagatcag
       61 cggccagttg ctggccaatc gccatttctt tttccattga ataaaattgg atgctgcctt
      121 tattgatatt gcgcttccca attttctcag gatcttcttt cgtatcaagc tttttggttg
      181 agttgtcagc cagcaggctc aggcctgggg caaagagcaa ccatatcaag agcagcgaaa
      241 acggacgtga ggctttcaaa aacataagct gaacccctcg aatgagccag aacgtggatg
      301 agtggtgaaa tagagaagga ccagggtcga aatctccaga tgtctcccaa caatagccac
      361 cattctagca aagtctagat tagaccaggg ttaatgatgg attagaatct tttaatctaa
      421 tgtcagcaaa cacttgccag aattgcaccc tgtggggtga tgtgctacat tcgcaagcca
      481 tctatgcctc cgcaaaacat caaatcctac atcgatattc agcaacttga tcaaatcagc
      541 aactttcgag aggccgaggg cgagcgggtt gttcgagcat accactttac cagcgaatta
      601 ggccggctgg tgtcccgtaa tcttgaggcg attgcctcgc ctcggacacc aggggccatt
      661 cggattattg ctggcccgcg cggcgttggg aaaagccatc tgttgatgct gattcaaacg
      721 cttgcgtata agccgcgtgc ccgggcgctt gtcagccagg tgcctcaggt ctcaaacgcc
      781 gttcggcgac ttgaaggaag tagctttgtc gttgttcagg tctcagggcc cactcgtggg
      841 gcaaccacct ttgatttcgg gattgccctg cgcgaagcac tcagacattc acctggaaac
      901 ccgatggagt ttgatgatac tcagtggtac ctggccattg aacacgggca ggtgtgtgaa
      961 ttgatctgca gtaagttatt tcctggcatg gttttgctgg tgacgattga cgatgtctcg
     1021 gaaatttttc gatcaggcaa acgcgaactc atcaccaata cctatcgctg gctgtcgtta
     1081 ctggccatga aagcccgtga cctgccaatt gttgttcttc tggcattaga tagtgatgtc
     1141 atcgacccgc aaaccggacc cgctgccaag ctgacagccg attatcaggt tgatgccctg
     1201 tcgcaggaaa gcttacggcg gatcgctgat caggcgctgt ttcgcaagaa cccgattcag
     1261 cgatctgaac tgcaggccct ctatcgtgag gcgttacgac tgctcccaaa ttttaagtgg
     1321 agtgaacagg agtttattga gatgtacccg gttcatccgg ttgtgctcga tgtatctcct
     1381 tccatgtgcc agtattgtga aagtttttca ctgtttggat ttatgaacgc cgccgccggg
     1441 cgggcgcttg ggcggcgacc gctccagtta atctcgcttg atgaattatt tgatcggttt
     1501 gaatttgatt tgcggcggaa cccggtgctg caacgcgggc tggaggttta tgattttctg
     1561 gccagcattg tgattcctac gctcgcgttt ggtgatcggc tcaaagcaaa gctgattctg
     1621 aaggggattt tattgtcgtc aatatcaggt cgaaccgtaa atgcctatga cctgaccgat
     1681 tcgctcatgt tgtatgaaga acggagtacg gatcgtgaat ctgcctatcg ccagacgttt
     1741 gccattttac agcaactgga aaacctggcc gaaggccagt tgatcggtaa ggaagaaggg
     1801 atctcccggc agtatcaaat cacaattgtg gagcggctcg atccagaagc aaaactcaat
     1861 gccgctgccg ctgagattgc ttttgatgac ccccggctgt gtgcgttgct ggtgacactg
     1921 ggcgggattt gttttcagga atggcctttt tcggctgacg ccttgctcat ggcaaatgct
     1981 gtggagcagc gaaccgaggt cacgggtacg tggcgtggca cgcatcgcaa aggcgttgtg
     2041 gtttttaacc agcccgttga actggccatg ttacaaccgg ttcagattga gcgtcatgaa
     2101 gaacgtttga ccccggatgc catctccgtg gatgctgagg tatttgaggt caaatcctct
     2161 gtcacaacac catccggatt acaggaagcc attacgactg aagtcaaggt gacatatacc
     2221 tatcagtggg cagaagaatg ttgtgagcta gactggcaga tttcgctctt gccagcgata
     2281 tatcctgaaa ttcctgacac cgtgccccca attccgagcc tgttattttg gaagccgggg
     2341 atgcttgacg ccgccggctg cgacatcctg aaaaaagcct gggttgtgta tacccgtggg
     2401 gaggaagttt tagggattga ggcggctgat cgcttacagt atcttgaaat tcaggttcgc
     2461 gagttatttc tcaagctcta tatcgaacag ggcctgttgg tcagcgcaac gggagaaaag
     2521 tattccgcca aaaatttctg gccggatggg acacttcaga aacggtttgt tgattttgtg
     2581 ggggtgttga ttcagacccc actggataaa cgatttccac tccacccggt ctttcaatca
     2641 gggctgaccg aacgtgaagt ccggcgactg gtccttggac tcatgggggg attgaaccca
     2701 acagatgctg cggttcagga atatgctctc agctatgccg taccattgca tattgtcact
     2761 gaacgggaag gggtttatca actggcggtt gatcgcgatg aagccctgag tcagcccttt
     2821 attagcgaag tgctccgact ggtggaagcc agcgcctcgg gtgacccgat accggtccag
     2881 gtgatttacc agacgttgcg ccgtgaacca tatgggttgc tggagccagt ccagcaattg
     2941 attatgatgg cgctgattgc cggttggcgc attgagttga ttgatgtttc ggggttgcgg
     3001 gtttttggcg ctgccgagtt aacccaggat gtcagctttc ggcaatatgg aacggtccgc
     3061 cggacagcga cgatcagcta ttcggctgaa gtgctcaccg aatggtgtcg gttactaact
     3121 gaacgaccag atctgccgga gctgacagcc gtgcaggccc ggaaaatgat tcgcgaggca
     3181 ctggctggct ggtatcagcg atgggctgac ttaaacctgg ccgagcggtt taattcattg
     3241 ccagttgaaa tgatgaccac ccgtacctgg cagttgatca ttacctgcaa gcgatatttt
     3301 gaaacaacct caaacgccgt tgaagcggtg ttgcacgaac ggatttcact ggaaatgggg
     3361 ctggctcgta ttgtcgaggt tttttccgcc aatccaacca tttttcagcg ggccagacat
     3421 gaatttcaac tgctggttga gtttctcaac tggctcccgt actacattcg ggccaagacc
     3481 tacaccctga cagccgttca gttgaataat gaggaaatca atgcccatcg gcgtgaactg
     3541 actcattttt ttgatcatcc ccaccggttg cttgatgaag ataagcgccg ccgatttgaa
     3601 gctacctttc ggagttttca ggataaattc accaacctgt acgccacaaa acacgaagac
     3661 gccatgacca aactggtcaa tctggaaacg ctcgaagagt ttctggcaag tcgtttgtgg
     3721 cgcacctttg agctggtcag ccagttaaac attgccaacc cgcgatttct ggttctggct
     3781 caggaaatga tcaagctgat tcgagaatca cattgtacgc ttccagtgcg ggatatctta
     3841 tcgattcagc cggcgtgctc gtgcgggttt cgggttgggt cggtgacacc catctcaacg
     3901 atggttgagg atttgaaaca actggttcgg accggggttg attatcatcg ccgattaatc
     3961 ttccagtacc gcccaaaact cagtcagaat ttacgaactg caaccacggc gacgggtagc
     4021 aataaagacc aggtgggtgc cctgctggca ttgatcagtt ccggagaatc aaccacggcc
     4081 atgaccatga ctccaggtat ggtcgagttg atcaatgaat ttctcaaaga ggaaacccgt
     4141 gtgattcctg cctctccgcc cgcaccgttt gtcctggggg atcggttgac gaaagctgag
     4201 ttaaaatcca gactgagttc ctggttggaa agtcttcctg atgatggaga tctggcattt
     4261 gaaatatctg aatcgccatt tgcaggggag aattaacggg ttgatttaat tgattcacac
     4321 acaaaagaag ggagcgtgat cattgatcac gctcccttct tttgtgtgaa ttccaacacg
     4381 ggtaacttag cgggaactgg tcaactggct ggctgattca actgtaaaat tggttttcgg
     4441 ggtcagggtt tgtcgcccga cccggtcagt aattgagacg gtaacttcat agtcaccagg
     4501 ttcaaggctg gttaaaggga ttgagcgtcc taaaatgatt tgctggccgc tcccagaaag
     4561 atcagaaatt ccattttttc catcttcttt gagccgcagg acttcctgtc catttttggt
     4621 gatgacatat tcaacatcga ccgctggctg gagcgtggtc tggtcaattt gcacattata
     4681 aacctgcaaa tagaccccca ggctttgatt ggttttgaaa acttgtgaca cattggggcg
     4741 aaccttgttg gatccaacaa taaaaggtcc ggctgctacc cgaccccgta atggttcgag
     4801 cagatccgca atgatcaaag aactggtgat cattttttcc tgactgtatt ggggaacttc
     4861 aaagccgtga tgaaccacgc cagtgtgccc gctggtgatg tcgcggacga ccatatcaat
     4921 cttgtatcgt ccagggggca ggaccactga tttttggtac acggagcttt gttgaatgcc
     4981 tgaatcaaat tgtgattcag ggaaggcaat cgtggctgga tcttcaaaaa taccggctcg
     5041 tttgcctgac agggccgtca cacgggcaaa aatattcacg gttgcctgct gaataccacc
     5101 tacgttcttg aaactcaggt cactattttt gacctgggtg gtgaacacca tgaccccttg
     5161 tgcctcacca gctcgaatat agtctgttcg gaggcttaag gggagcacat caaattcagt
     5221 taactgatca actttttcaa gtttatcagc cagtgcccgg tatttgacct ggggcgccct
     5281 ttgaaggttg gcatacaggg ataaccgttc gaatggctgg tcctgtgccc gccggacaaa
     5341 ttgagaactg ttactgttcc cagggctaaa gtaaggccgg tcacgtttgg aggtaatccc
     5401 aagctgttcg tacaaggtta acccgccgtt cggggtaaac aacaaggcgt ccttttcatc
     5461 cgggctttgc gcaatccggt actctccgct tccactggga tctacaaact caatttcaat
     5521 gccggaccca atcccatcaa taaagcggta gaaccagatt tcaaatggga atgtggaggt
     5581 ttgccccccg ccctgataat acgggcgttc atagggcccc cctgccgggt gggattcaat
     5641 actgtcgggc ggtccaaatg taatgtagat ccgaccccgg tctgttttcc atcctggtat
     5701 cccggatgaa aaccgctcat tcgcataggc aattcggcga taatactctt ccaggtattc
     5761 attttcggtg gtatctggat ctggatcccg ccgcagccag aattgttcaa tgaactcctc
     5821 gcgttcttca tcggtaagga gcttcttgaa ggcatcgcgt tcttccttgg tgatgatgta
     5881 ccggacatct tcatcaagcc atttttgata gactttctca acttcaactt tccgtttctg
     5941 cttcttcttt tcaccttctt gtctggccag taacgggttg gttgaggcca gacacaagaa
     6001 catcaaaaca aaacaactga tgcgatacgc aaatctgcgt gatgttgaaa aaggatgtgg
     6061 gtggttcata gtcataggta gaattccttg cagaaatcgg gctgttggga aggcaacaac
     6121 cctgaggccc gtctatttga gcaattcacc ggagcattcg gtgggtattg taattatgca
     6181 cgaagaaggt tcaacccaca ggattactct tgagcggtgg cgacggctgt tacaggcatt
     6241 gagtcagtca atccagcacg ccgacgaaga acaaaggtga caagaataaa taacaaacct
     6301 gccaggccat tggcaataat ggtcagaaaa agggcactcc gcaagccgcc agtggtactt
     6361 agccgatctg aaagatatcc aatgatcgct ggtgccggtg tatcacccag gatgtgaatg
     6421 ccgaacatat agagagcaat ggctgttccg cgaaggctgg cgggtacaat ctcacctaca
     6481 atggcagcca ctgggccatg ataccagccc ataaaataag agataaagaa ggttgcaatt
     6541 acaaactcag ttgaattggt ggtattgatc ccccaaatca taaagggaac ggtgatcagg
     6601 aatgcaaat
//
LOCUS       DAOQDO010000039         6608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_6848_length_6608_cov_2.998169, whole genome shotgun sequence.
ACCESSION   DAOQDO010000039 DAOQDO010000000
VERSION     DAOQDO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6848_length_6608_cov_2.998169"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2708
                     /locus_tag="PKZ53_01200"
     CDS             69..2708
                     /locus_tag="PKZ53_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39078.1"
                     /translation="MSNSIFSNGNLGIDLQDDGPTPNDSGDSDGGPNLLQNFPTLTSA
                     ISDTGTTTITGMLDSEPNTSYRIEFFTNPSCDGSGFGEGQTFRGFLNVTTGTDGLAPI
                     SLVVNSVLTGVVTATATTTVAPFNTSEFSPCEPVVTNNTPPVLGNYNSATVQEGGSIT
                     VSPSAAPTDDGTIVSLTATAPGYTGGINADRLTGNIAFSTAKPSGQYTVTVTAIDNNG
                     VDTETSFTLTVVNVAPTVIVNSPITRQQGSPGSTSLIAQVSDIGTPAQQLSVSVINVP
                     AGLTISNISNSGGNVSAFIEASCASTPGQNTVLLLVADNSLVKQTNLVINVLPGTANL
                     GTYLDNSVNEGSSVTVAPNTPPSSLKPNSLVVTVTPNTFAGLIRFTPEAGTINITNAR
                     PYGEYTVQISAEGTCSGTISRSFLLTVNNVAPTITPAAPVTIKQGSQRAVFPVAAVGD
                     SGTDPGDLVVRAVNLPEGILFAQITNEDGLVTAEIEAGCAAPVGTIPVQLEVSDGNLT
                     STAQFSVNVAENKVPTLGSYQNVFLEQLTSFTFIPSAAPFDPDGNLTKVEVVPTNLPD
                     GGTININSGSGVVTVTAMDDQKLLGDYPIEIRATDACGLVAVQRVVLTIELPAPSNLR
                     ALRVAASRVDLSWADNSAGEGGFVIERRTATTPFTLVSTVPANSRTFSDGRVTAGEAY
                     TYRIKANKTAGDSLPSNEAAVILSTVTLDQFYPVASTPGKTITVYGAGFQNGTEVFFG
                     GDRRIPATEVLVVSSTRLQVTIPASQQSSNINGFLTIAVPGAAEITTQSLSNTDELPL
                     PGAPVLSEFVLIGDANGDGAVTAVDLTTTFAILLGQIAPLPRQVFAADVYSFNPDGSY
                     GDGKLDALDITVLRAVLLGQITL"
     gene            2725..3201
                     /locus_tag="PKZ53_01205"
     CDS             2725..3201
                     /locus_tag="PKZ53_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ39079.1"
                     /translation="MFRAVLMVLILVGTGVAGFAQNKSRLDREIEALLQVHKKDRQAH
                     FETDARKLMEHAADDFIAVSNGTIQRSTRAEVLKTFEAYFQNAKYFEWDDLEPPIVRV
                     SNDASLAWMIVRTRVRRTQKQADGSDKERSFVYAGIMTYEKKKGKWVRVANVSTFE"
     gene            complement(3294..5639)
                     /locus_tag="PKZ53_01210"
     CDS             complement(3294..5639)
                     /locus_tag="PKZ53_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ39080.1"
                     /translation="MKKLPFMQHLGLGIILVILMGIKTVASPGSSPDQAPDPIPQTQC
                     HQLQCDLLEQQARTEFVAGHYLKAASYLLEAQSQGCKSQVLNYLSGKILEVYQPDFML
                     ASEWTIGFTQTGELVSIDKGKVTFWNVTTRQQVREVFLDDAATLVTDVNQYHAVLILS
                     PDGKRLISTDNTDTIKMWELTTGKCIWRFSENKEPVRAVLNDQGTLLLVTCDSGQAYV
                     LDSLTGKKVLTLPQTSGPLGKGCFSHHGKWIVTGAYDGTITIWDLHTGQKIRSLTGHT
                     GFVLKVCFDSSDQRLLSGSRDQTARLWNPETGDCLQTFLHDNVLSPQVGTFNHRGNQV
                     VTVSKDRSARIWDVTTGKLLFRLQHQNWVLQAVFAQDDTCLVTVEGTQKDGFLKVWNP
                     TTGVLQASYGIPGNPYYQAFAGFSPDGKWIASLTGRLWQIHHLDQQIDFPEDAQCLEM
                     SADGKWVVAAGPRLGFVRMNLETNERTMLAQADTFQPRAVCVSQDGQTAGGIWEQVQE
                     RMLRLFLPSASQVRWYRSGKQVGEIVATSGWYTHFFFSESANQAVLVRFVPRSDQSPP
                     RSVVEVWNLAANIKQTEFTLDRELGTVSALSQGFSKTEMVIGTNLGTVLMYDFNGKQT
                     GALSTYHSGNITSVSISSDGKGVLSSSEFDSTIEVWDVETKQLLTFVENPESTVPLVR
                     FLPHSPLIISLTESGAFGMVQLMDSRTGKTLFSAQTPSKLAGFVGCSPKNLLFLEDPR
                     TLRILKISLDPPSATELKTVLSPFNPYQVINHLGLIPIQNQ"
     gene            complement(5912..6391)
                     /locus_tag="PKZ53_01215"
     CDS             complement(5912..6391)
                     /locus_tag="PKZ53_01215"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNJ39081.1"
                     /translation="MKRTNLTIAFLILGLSFPVMSFGGVQEKRKIGRGDIPTPIKEAF
                     TRAWPKAKIKQCEVETTANRAVYFIEAERDDREIEAVYQLEITLLEEQEDIDLKEVPS
                     VVIEAIKAAYPAGEIEEVEKITRQGTLLGYEVELKDGKQNIELRLDPSGKILERKDD"
BASE COUNT         1623 a         1695 c         1601 g         1689 t
ORIGIN      
        1 gactgatgct caaacgcctg gctccggcct cagtctgaat ttccaaacag tctcttagta
       61 acagcatttt gtcaaattcc attttttcca acgggaattt aggcattgat ttgcaggatg
      121 atggcccgac tccgaatgat tcaggtgaca gtgatggtgg gccaaacttg ctccaaaatt
      181 tcccaaccct gacttcggca atctcagaca ccggtaccac cacgatcact ggaatgctcg
      241 atagcgagcc aaataccagc tatcggattg aatttttcac gaatccatcg tgtgatggct
      301 ctgggtttgg cgaaggacaa accttccggg gatttctcaa tgtcaccacg ggcaccgatg
      361 gcctggcccc aatttcactg gtggtcaatt cggtgctcac gggggtcgtc acagcgaccg
      421 caaccacaac ggtggcgccg tttaacacct ctgaattttc gccttgtgaa ccagttgtca
      481 ccaacaacac cccgccagtg cttggaaact acaactcagc cacggttcaa gaaggtggtt
      541 cgattaccgt ttctccgtcg gcagcaccaa ctgacgatgg aacgattgtc tcgttgacgg
      601 caaccgcccc tggctacact ggcggtatca atgcggatcg cctgaccgga aatatcgctt
      661 tttcaaccgc taaaccttca ggtcaatata ccgtcaccgt gaccgcgatt gataataatg
      721 gggtggatac cgaaacgtcc tttaccttga ccgtggtcaa tgtcgcccca accgttatcg
      781 tcaacagtcc gattactcgc cagcagggct cgcccggttc aacttcattg attgctcaag
      841 tcagtgacat cgggacgccg gctcagcaac tctcagtttc ggtcatcaac gtccctgcag
      901 gtctcacaat tagtaatatc agcaattcag ggggcaatgt ctcagccttt attgaagcgt
      961 cttgtgcgtc aacgccgggt caaaatacgg tactgctgct ggtggcagat aacagtctgg
     1021 taaagcagac gaatctggtc atcaatgtcc ttccaggaac ggcgaatctc gggacatatc
     1081 tggacaattc agtgaatgaa ggcagttcgg tgacggtggc gcccaataca ccaccatcaa
     1141 gcctgaaacc caattcgctg gtcgtcaccg tcaccccaaa cacttttgct ggactcatcc
     1201 ggtttacgcc cgaagccgga acgatcaaca tcaccaacgc ccggccctat ggagaataca
     1261 ctgttcaaat ctcagccgag ggcacctgtt caggcaccat ttcacgcagt tttcttttga
     1321 cggttaacaa tgtcgcccca accattaccc cagccgcacc ggttacgata aaacagggaa
     1381 gccagcgcgc tgttttccca gtcgccgccg tcggtgattc gggaaccgac cctggcgatc
     1441 tggtggtcag agctgtaaat ctgcctgaag gaattctctt cgcccaaatt accaatgaag
     1501 atgggctggt tacggctgaa attgaagccg gttgtgccgc accggttggg acaattccag
     1561 ttcagcttga ggtttcagat ggcaatttga ccagcaccgc ccaattttca gtgaatgtgg
     1621 ccgagaataa ggtgccaacg ctggggagct atcaaaacgt ttttctggaa caactgacct
     1681 cgtttacctt tattccgtca gccgccccgt ttgatccgga tggaaacctg accaaagtcg
     1741 aagtggtgcc aaccaacctg cctgatggcg gcaccatcaa catcaattct ggctctggag
     1801 tcgttaccgt cacggccatg gatgatcaaa agctcctggg tgattatcca attgaaatca
     1861 gagcaactga cgcctgtgga ttggtggcgg tgcaacgcgt cgttttgaca attgagcttc
     1921 cagcgccatc aaatttgcga gccctgcggg ttgctgcttc acgagttgat ttgagctggg
     1981 cagacaatag cgcgggtgaa ggcggctttg tgattgaacg ccgaacagca accactccgt
     2041 ttactcttgt ttccaccgtt ccggccaatt cccgaacctt tagcgatggc cgggtcaccg
     2101 ccggtgaagc ctatacctac cgcatcaagg ccaataaaac ggcgggtgat tcgctcccat
     2161 ctaatgaagc ggcggttatc ctgtcaacgg taacgcttga ccagttttat cccgtagctt
     2221 caacgccagg aaagaccatt accgtctatg gggctggctt ccagaatgga accgaagtct
     2281 ttttcggagg cgaccgccgg attccagcca ctgaggttct ggtggtaagc tcaactcgac
     2341 tgcaagtcac gattcctgcg tcacagcagt catccaacat caatggattt ctcacgattg
     2401 ctgttccagg cgccgccgaa attacaactc aatcgttgtc caacacggat gaactgccgc
     2461 tgccaggtgc ccctgtcctg tcagagtttg tattgattgg agatgcgaac ggggatggcg
     2521 ccgtcaccgc cgttgatctg accacgacgt ttgcgatctt gcttggccag attgccccgc
     2581 tcccccgtca ggtttttgcc gcagatgtct attcattcaa cccggatggg agctacggtg
     2641 acgggaaact ggatgccctc gacattactg ttttgcgagc tgtcctgtta ggccaaatca
     2701 ccctgtgaag gtgaaaggaa ccctatgttt cgtgctgttc tcatggtgtt gattctggtt
     2761 gggactggtg tcgctggttt tgcccaaaat aagtccaggc tggaccgtga aattgaagcc
     2821 ctgctccagg ttcacaaaaa agaccgccag gctcattttg aaaccgatgc ccgaaaattg
     2881 atggaacacg ccgccgatga ctttattgcg gtttccaatg gcacaattca acggtcaact
     2941 cgtgccgagg tgctcaagac atttgaggcg tattttcaga acgcaaagta ttttgaatgg
     3001 gatgacctgg aaccaccgat tgttcgggtc tcgaacgatg ccagcctggc ctggatgatc
     3061 gttcgaaccc gagttcggcg gacccaaaaa caggcagatg ggagcgacaa agaacggtct
     3121 tttgtgtatg cgggcattat gacctatgag aaaaagaagg ggaaatgggt ccgtgtggcc
     3181 aatgtctcaa cctttgagtg agttttgaaa gaaaacagct cattattaga gttgttgcga
     3241 cgggaaaaac atcacaatct gagtttctct gagggtccta tttcctgatg gggtcattga
     3301 ttttgtattg gaatcaatcc aaggtggttt atgacctggt acgggttaaa aggcgagagg
     3361 accgttttga gttctgtcgc cgatggtggg tccagtgaaa ttttcaggat tcgcaaggtt
     3421 cggggatctt ccagaaacag taaatttttc ggtgagcacc cgacaaaacc ggccaattta
     3481 ctcggagtct gagcagaaaa caacgtcttt ccagttctgg aatccatcag ttgaaccata
     3541 ccgaaagccc cgctctctgt cagggaaatg ataagggggg aatgagggag aaatcgaacc
     3601 agagggactg tgctttcagg gttttcaaca aaagtgagta attgtttggt ctcgacatcc
     3661 cagacttcaa ttgttgaatc aaattctgaa gacgaaagca ccccttttcc atctgaactg
     3721 attgaaacag aagtgatatt ccctgagtga taggtactca gtgcccccgt ttgcttccca
     3781 ttgaaatcat acatgaggac ggtgcccaaa tttgtcccga tcaccatttc ggtttttgag
     3841 aaaccttgac tgagggctga gacagtcccc agttcccggt ccagagtgaa ctcagtttgt
     3901 ttgatgttgg cggctagatt ccagacttca accactgacc tgggcggaga ttggtcagag
     3961 cggggaacaa atctgaccaa caccgcctgg ttggctgact cactgaagaa aaaatgagta
     4021 taccaacctg aagtggccac gatttcccca acctgtttac ctgaccgata ccacctcacc
     4081 tgtgaggctg atgggagaaa caaacgtagc attcgttcct ggacttgttc ccaaatcccc
     4141 cctgctgttt gaccatcctg gctcacgcaa accgcccggg gttggaatgt atcggcctgg
     4201 gcaagcatcg tacgctcgtt ggtttctaga ttcattcgga caaagcccag cctgggtccc
     4261 gcagcaacca cccattttcc atccgcactc atttcgaggc attgggcatc ctctgggaaa
     4321 tctatctgct ggtctaaatg atgaatctgc cagagccgcc cggtgagaga agctatccac
     4381 tttccatcgg ggctaaatcc tgcgaatgcc tggtagtatg gatttccagg gattccataa
     4441 cttgcttgca ataccccagt tgtgggattc cataccttta aaaatccatc tttctgagtt
     4501 ccttctactg tgaccaggca ggtatcatcc tgagcaaaaa cggcttgcaa cacccaattc
     4561 tgatgttgaa ggcgaaagag taattttccg gttgtcacat cccaaatccg tgcgcttcga
     4621 tctttgctga cagttaccac ttgattgccc cgatggttaa atgttccaac ctggggggat
     4681 aaaacattat catggaggaa ggtctgtaaa cagtcgccag tttccgggtt ccagagtcgg
     4741 gctgtttgat cacgacttcc agaaagtaag cgttggtctg aactatcaaa acacactttt
     4801 aaaacaaacc cggtgtgccc ggttaatgag cgaatcttct gcccggtgtg taaatcccaa
     4861 atcgtaattg tcccatcata ggctccggtc acaatccact ttccatgatg actaaaacac
     4921 ccttttccca gcgggccact ggtttgaggg agtgtcagga ctttctttcc cgtcaatgaa
     4981 tcaaggacat atgcttgacc tgagtcacag gtcaccagca agagggttcc ctgatcgttg
     5041 agaactgccc tcactggctc cttgttttca gaaaagcgcc agatacattt ccctgtcgtc
     5101 agttcccaca tttttatcgt gtcggtgtta tcagtcgaaa tgagccgctt tccgtctggt
     5161 gaaagaatca agacggcgtg atattgattg acgtcggtta ccagagtcgc agcatcgtca
     5221 aggaaaactt ctcgcacttg ctggcgagtc gtgacattcc aaaaggtaac tttcccttta
     5281 tcgattgata ccagctcacc agtttgggta aatccgatgg tccattcgct ggcaagcata
     5341 aaatcgggct gatagacctc caatattttt cctgagagat aattcaacac ctgagatttg
     5401 cacccctgag attgcgcttc taaaaggtaa gaggccgctt ttaaatagtg ccctgccaca
     5461 aactcggttc gagcttgttg ttcaagcaaa tcacattgaa gttgatgaca ttgagtttgg
     5521 ggtattgggt ctggcgcttg atcaggcgaa gagccaggtg acgcgactgt ctttattccc
     5581 atcaggataa ccaaaataat tcccagccca agatgctgca taaaaggtaa ttttttcatg
     5641 tctggttccc aaaaagtcaa taactggagt tgatcaaagg cgatctgaga aattgccgga
     5701 gttgcttacg gcaagctacc tgaagatctt tggcaaagag aaatctcgtt tggctacgca
     5761 gtgcatacca gaaccaaaga cttacccaaa gcgattgatt acaaagggct tggcctggat
     5821 gtgtaaatcc cagccagtga aacggaccca gtccagttca ggggggccca cgtcggaaaa
     5881 aagaaatcta actaacggca tcggtcgaag tttaatcgtc cttccgctcc agaatcttgc
     5941 cagatggatc cagacggagt tcgatgttct gttttccgtc tttgagttca acctcatacc
     6001 ccaacagtgt gccctgacgg gtaatttttt caacttcctc gatctctcca gccgggtacg
     6061 ctgccttgat ggcctcaatc acgactgaag gcacctcttt taaatcaata tcttcctgct
     6121 cttcgagcaa cgtgatttcg agttgataca cagcttcgat ttcccggtca tcgcgctcgg
     6181 cttcaataaa atacactgcc cggttggctg tggtttcaac ctcacattgt ttgatttttg
     6241 cttttggcca ggcacgggtg aacgcctctt tgatgggcgt cgggatatcc cctcgtccga
     6301 tttttcgctt ttcctgaaca cctccaaaac tcatgacggg aaaagacagt cctaaaataa
     6361 ggaaggctat cgtcagattg gttcttttca taaagtctcc tttcgatttc agatgagaaa
     6421 attttcagtg tgggaatcat tgtcccaact gaagacagag gaaagctggt cttcatcagg
     6481 aaaattcctc ccttttcatc ctatcttcgg cgtggatacc cggttcttta gagccgagag
     6541 gaaacgccgc tcctttattg tgagaagtga aagtggaagg atttgactcc tcccacttgt
     6601 gactcttg
//
LOCUS       DAOQDO010000040         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7003_length_6518_cov_3.062510, whole genome shotgun sequence.
ACCESSION   DAOQDO010000040 DAOQDO010000000
VERSION     DAOQDO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_6518_cov_3.062510"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="secY"
                     /locus_tag="PKZ53_01220"
     CDS             <1..1216
                     /gene="secY"
                     /locus_tag="PKZ53_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNJ39082.1"
                     /translation="VLDLFSGGNFRVVSIFALGITPYITSSIILQLMTVVWPYLKKLQ
                     EEGELGRRKINQYTRYLTIVLSLVQSLTIALWLNGQPGLVIGGSGVWFIFVTMVTLST
                     GAAFVMWVGEQITERGIGNGISLLIFAGIVIRLPDALRQLVTKAQSDPTSALSVIILL
                     VVMVAVIAGVVFMERGQRKISIHYARRQVGKQQTAGHSTYMPLRLNMAGGIPVIFASS
                     ILTFPQTVAQFIKSEYVQNLTQMLRTGHPLYDLIFVSSIIFFTFFYVSIVFNTDEVAE
                     NLRKYGGFIPGIRPGKRTADYLNSVLTRLTTVGAIYLAVVCIIPQFIITGFEVSALPL
                     IGEKLYTLLNSHPATSWIIKGLGFNFYFGGTSLLIVVGVAMDTVQQIESQLIMRHYDG
                     FLRKGRIRGRRG"
     gene            1301..1969
                     /locus_tag="PKZ53_01225"
     CDS             1301..1969
                     /locus_tag="PKZ53_01225"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNJ39083.1"
                     /translation="MATTVILMGAPGAGKGTQARLITEVFGFPHISTGELLREIAKQE
                     TPLAERVRQVLATGKFVSDEILADLVTERTSRSDCQNGYILDGYPRTRPQLEFLETLV
                     HRQGTRLIAIEISVPREVLFQRLTGRRTCGQCGAIYNIYFVPPGTNGTCHQCGSSNLQ
                     IRDDDQEEVVDTRLTNYAELTEPLIRSFQEKGVLRVIDGNQEVEAAFAQLKRALESES
                     AAAW"
     gene            1985..2737
                     /gene="map"
                     /locus_tag="PKZ53_01230"
     CDS             1985..2737
                     /gene="map"
                     /locus_tag="PKZ53_01230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNJ39084.1"
                     /translation="MVIAKSKAEIEKMYRAGQLVGTLLKELCAMAQPGVTTEDLDRFA
                     ISWLKERNAISPFKGYRGYPKHICASINDEIVHGIPSPSRILKDGDIISIDCGATLDG
                     YVADTAATIGVGAISDSARTLIKHTEASLFQAVGQMQTGNRLYDISYAVQSYIEPLGY
                     SLVREFCGHGIGRRMHEDPQVPNRGRPNTGQRLRVGWVLAVEPMVNAGTHAIRVLNDG
                     WTAVTLDGQLSAHFEHTIAITEEGPRVLTLVD"
     gene            2863..3081
                     /gene="infA"
                     /locus_tag="PKZ53_01235"
     CDS             2863..3081
                     /gene="infA"
                     /locus_tag="PKZ53_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNJ39085.1"
                     /translation="MSKEDSIEVMASVLEALPNATFRVELENKHKVLARISGKMRKNF
                     IKILPGDKVLVELSPYDLTRGRIVYRYK"
     gene            3174..3287
                     /gene="rpmJ"
                     /locus_tag="PKZ53_01240"
     CDS             3174..3287
                     /gene="rpmJ"
                     /locus_tag="PKZ53_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNJ39086.1"
                     /translation="MKVRASVKKICDKCKLIHRKGIVRVICENPKHKQRQG"
     gene            3371..3754
                     /gene="rpsM"
                     /locus_tag="PKZ53_01245"
     CDS             3371..3754
                     /gene="rpsM"
                     /locus_tag="PKZ53_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNJ39087.1"
                     /translation="MARVAGIDLPQNKRAEIGLTYIYGIGRSRANLILEEANVDKNRR
                     IRDLSEDELTRIRTVIDRQGDVEGDLRKRIQLDIKRLVEIQCYRGIRHRKGLPVRGQR
                     THTNARTRKGPRRATVAKKKAPGKK"
     gene            3841..4260
                     /gene="rpsK"
                     /locus_tag="PKZ53_01250"
     CDS             3841..4260
                     /gene="rpsK"
                     /locus_tag="PKZ53_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNJ39088.1"
                     /translation="MAKKTTTAGKAGAKKKIFKKRERKNVPQGIVHIQATFNNTLVSI
                     TDLDGNLVSASSAGALGFKGSRKGTPFAAQQAAARAAIQAKENGMQLAEVRVKGPGSG
                     RESAIRAVQAAGIDVKVIKDVTPIPHNGCRPRKRRRV"
     gene            4352..4987
                     /gene="rpsD"
                     /locus_tag="PKZ53_01255"
     CDS             4352..4987
                     /gene="rpsD"
                     /locus_tag="PKZ53_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNJ39089.1"
                     /translation="MARYRGPVCRLCRREGVKLFLKGERCFKPSCAIDKRKKLPGQHG
                     EARRGKLTGYGEQLREKQKVKRMYGMLEGQFRSCFERAVRQKGVTGENLLSSLERRLD
                     NVVYRMGFAASRAQARQLVNHGHIRVNDRKVNISSFVVKVGDTVSVRDGSLTNVHILN
                     AFQTAGGRGVRPAWVEIVDAAKLAGKIVSLPRREDIDKDIREQMIVELYSK"
     gene            5079..6071
                     /locus_tag="PKZ53_01260"
     CDS             5079..6071
                     /locus_tag="PKZ53_01260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNJ39090.1"
                     /translation="MWTGFQKPKRLACDFETLTDKYGRFYAQPFERGFGTTIGNSLRR
                     ALLSSIEGAAITAVKIEGVLHEFSSIPGVTEDATDIILNLKRIPFLMTGAGVKTIRLE
                     SNTPGVVYSRDIEVGSDIEVLDGSIPIATVSEGGRLSIEMRLKMGRGYVPADRNTDED
                     LSVGYIPIDSVHSPIKKVNYQVEAARLGQDTDYDKLTIEVWTNGSIKPDDAIGLAAKL
                     IKDHMTIFINFDDTEPEMKEPEIIDRPKRTVNEYLDRSVEELELSVRSYNCLKNADIR
                     TIRELVQKTEQEMLKTKNFGRKSLNEIKEIMTAMGLRLGTIFDEEGNIVGYRDE"
     gene            6122..>6518
                     /gene="rplQ"
                     /locus_tag="PKZ53_01265"
     CDS             6122..>6518
                     /gene="rplQ"
                     /locus_tag="PKZ53_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNJ39091.1"
                     /translation="MRHLNAHRKLGRTSAHRKSLLRNLATSLIIEERLVTTLPKAKEL
                     RPFVERAITLGKRGDLHARRLAASYFHAGNQNRIPDGGRGHQRSPRTAGVLAVSKLFD
                     TIAARFGDRPGGYTRIVKLGHRRGDGAEMA"
BASE COUNT         1639 a         1533 c         1733 g         1613 t
ORIGIN      
        1 ggtgcttgac cttttttcag gcggcaactt tcgggtggtt tctatttttg cgttgggaat
       61 taccccctac atcacgtcct cgattattct ccagttgatg acggtggttt ggccctacct
      121 caagaaattg caggaagaag gtgaattagg gcgccggaaa atcaaccagt atactcggta
      181 tctgaccatt gtgttgtcat tggtgcagtc tttaacgatt gccctgtggt taaacggcca
      241 gccagggctg gtcattggcg gttcaggtgt ctggtttatc tttgtgacaa tggttactct
      301 gagcaccggg gcagcctttg tgatgtgggt tggtgaacaa atcaccgagc gggggattgg
      361 gaatggaatc agtctgctga tttttgcagg gattgtgatc cgcctgcctg acgcgctgcg
      421 ccaattggtg accaaagccc agtccgaccc aacatccgcc ctcagcgtga ttattttgct
      481 ggtagttatg gtggccgtca ttgccggtgt ggtgtttatg gagcggggac agcgtaaaat
      541 ctcgatccat tatgctcgcc gtcaggttgg aaaacagcaa acggcgggtc actcaactta
      601 catgccgctc cggctcaata tggctggggg gattcccgtg atttttgctt catcaattct
      661 gacttttcca cagaccgtgg cccagttcat taagtcagaa tatgtccaga acttgaccca
      721 gatgttgcga actggacatc ccctctacga tctgattttt gtctcttcga tcattttctt
      781 taccttcttc tatgtttcca ttgtgtttaa caccgatgaa gttgcagaaa atcttcgcaa
      841 atacggcggg tttatccctg ggattcgacc tgggaaacga acggctgatt acctgaactc
      901 ggtgttgacg cggttgacca cagtcggagc aatttattta gctgttgtct gtatcatccc
      961 tcaatttatc atcaccgggt ttgaagtcag cgccttacca ttgattggtg aaaaactcta
     1021 tacattgctg aactcacacc ctgccacaag ctggattatc aaaggattag gtttcaactt
     1081 ttactttggt ggaacttcac ttttgattgt ggtgggtgtt gcaatggata ctgtccagca
     1141 aattgaatcg cagttgatta tgcggcatta cgacgggttc ctgcgcaagg gccgaatccg
     1201 tggccggcga gggtaaccca ggactcaatc cagccagcca ctttcctgac tggctgaccc
     1261 gctctggcgg gtatgtcatg ggaaagaaat tggagggaat gtggctacca cagttatctt
     1321 gatgggagca ccaggggccg gcaaaggaac gcaggctcga ctgataactg aagttttcgg
     1381 atttccccac atttcaacgg gcgagttgct tcgtgaaatt gcaaaacaag aaacaccact
     1441 cgctgagcgt gtgcgccagg tcctggcgac tggaaaattc gtttccgatg aaatactggc
     1501 tgacctcgtt accgagcgta ccagccgcag tgattgtcag aatggatata tcctggacgg
     1561 gtacccccga accaggccgc aactggaatt tttagaaact ttagttcacc gacagggaac
     1621 ccgtctgatt gcgattgaaa tttcagttcc acgggaagtg ttgtttcaac gcttgacggg
     1681 gcgacggact tgcggtcagt gcggcgccat ttacaatata tattttgttc cgccggggac
     1741 aaatggcacg tgccatcaat gtggatccag taacttgcaa atacgggatg atgatcagga
     1801 agaggtggtt gatacccgac tgaccaatta tgctgagtta actgagcctc tcatccgatc
     1861 ctttcaggaa aaaggagtct tacgcgtgat tgatggaaat caggaagtcg aggctgcctt
     1921 tgctcagtta aaacgggcgc tcgaatctga gtctgctgct gcgtggtgag agaaacgata
     1981 cggtatggtc attgccaaat caaaagcaga aattgaaaag atgtatcggg ccggccaact
     2041 ggtcggaacg ctgctcaagg aactttgtgc gatggcacaa ccgggcgtga ccaccgaaga
     2101 tcttgatcgt ttcgccattt cctggctcaa agaacgaaat gccatttcgc catttaaggg
     2161 gtaccgggga tatccaaagc atatttgcgc atcaattaat gatgaaattg ttcatggtat
     2221 cccctcacca tcgcgaatcc tcaaagatgg tgacatcatt agcattgact gtggcgccac
     2281 cctggacgga tacgttgccg atacggctgc aaccattgga gttggggcga tttccgactc
     2341 tgcccgaacg ttgatcaagc ataccgaagc gtcgctgttt caggccgttg gtcagatgca
     2401 gaccgggaac cgactctacg atatctcata tgccgttcaa tcatacattg agccgctggg
     2461 atatagcctg gttcgagagt tttgtggtca tggaattggc cgtcgaatgc acgaagaccc
     2521 gcaggtgccc aatcggggac gtccaaatac tggacagcgg ctccgggtag ggtgggtctt
     2581 agccgttgag ccgatggtca atgccggcac ccatgccatc cgggtcttaa atgacggatg
     2641 gaccgctgtc accttagatg gccagctttc agcccacttt gaacatacca tcgccataac
     2701 cgaagagggg ccacgagttt taaccctggt tgattgattg agggacataa tcctttacaa
     2761 ccgcttcttt tttatgctag tctgcgcggt tcgctaaact cccgccactt ttcctaagtg
     2821 caaatcatca tttcaaccca taacgctcca ctgaaggggg atatgtctaa ggaagattct
     2881 attgaagtca tggcgtcggt acttgaagcc ctgcctaatg ctacatttcg cgtcgagctt
     2941 gagaataaac acaaggtatt agcccgaatt tctgggaaaa tgcgcaagaa ttttatcaaa
     3001 attttgccgg gggataaggt tctggtcgag ctttcgccct atgacctgac ccgaggccgg
     3061 atcgtgtatc gatacaagta attcatatca aagactgaaa actttggtgc ctgcggtttc
     3121 atttgaaacc accagagatc actattgctt ttttcattgt tgccggggga gttatgaaag
     3181 tacgtgcgtc agtaaaaaaa atctgcgata agtgcaaact cattcatcgc aaagggattg
     3241 tgcgcgttat ctgcgagaat ccaaaacaca agcagcgtca aggataaccc tcctcaacga
     3301 ggagtggcct cacgcctgaa attaattgca accaacggaa gttcaaccat tttgactggg
     3361 aatacgcaac atggcacgtg tagctggaat tgatttacct caaaataaac gcgccgaaat
     3421 cggtttaacc tacatttacg gcatcggacg ttcacgcgcg aacctgattc ttgaggaagc
     3481 gaatgtcgat aaaaaccgga gaatccggga tctgagcgaa gatgaattga cccggattcg
     3541 caccgtaatt gatcgtcagg gcgatgttga aggcgatttg cgcaaacgga ttcaactcga
     3601 cattaagcgc ctggtagaaa ttcaatgcta tcgcgggatt cgtcaccgca aaggtttgcc
     3661 ggtccgtgga cagcggacac ataccaatgc tcgtacccgc aaaggtccac gtcgggcgac
     3721 ggtggccaag aagaaagccc ctggcaagaa gtaagatgct ggtgtcattt gacacgggca
     3781 agccactttg gtggtctact tcaggattaa tttactgttt atcacttgag taagaataca
     3841 atggccaaga agacgacaac tgctggaaaa gcgggagcta aaaagaagat tttcaaaaaa
     3901 cgggaacgca aaaatgtgcc gcagggaatt gtgcacattc aagcaacgtt caataacacg
     3961 ctcgtcagca ttaccgattt ggatggaaat ctggtgagcg ccagcagtgc tggagcgctg
     4021 ggctttaagg gatctcggaa gggaactccg tttgctgccc aacaggctgc ggcacgagct
     4081 gcgattcagg caaaggaaaa tggcatgcaa ctggccgaag tccgggtcaa aggcccaggt
     4141 tcaggtcgtg aatcagccat ccgtgccgtt caggctgctg gtatcgacgt aaaagtcatt
     4201 aaagatgtga cacctatccc ccacaatgga tgtcgaccgc gcaagcgtcg tcgggtctaa
     4261 tgtggtgtgg tcggctgttt caccataacg cgaactctat tgatttcttg agatgtgaaa
     4321 gcccgcacca aatgggcttg ggagggattg cttggcaagg tatcgtggtc ctgtgtgtcg
     4381 tttgtgtcgc cgagaaggcg tgaagctttt tttgaaaggt gaacgatgtt tcaaaccttc
     4441 ttgtgcaatt gataagcgaa agaaacttcc ggggcagcat ggcgaggccc gtcgtggaaa
     4501 gttgactggc tatggcgaac aattgcgtga aaaacagaaa gtaaagcgca tgtatggaat
     4561 gctcgaaggc cagtttcggt cttgttttga gcgcgctgtc cgccaaaaag gggttacagg
     4621 tgaaaacctg ttatcgagcc ttgagcgtcg gcttgacaac gttgtgtacc ggatgggctt
     4681 tgctgcctcg cgagcgcagg ctcggcaact ggtcaaccac gggcatatcc gggtcaatga
     4741 ccgcaaagtc aacatctcat cctttgtggt caaagttgga gacaccgtca gcgtacggga
     4801 tggcagcctg accaatgttc atatcctgaa tgccttccaa acggcgggag gtcgtggcgt
     4861 tcgtccagcc tgggttgaaa ttgttgacgc ggccaagctg gctggaaaaa ttgtttccct
     4921 gccacgccgc gaagatatcg acaaagatat ccgggagcag atgattgtcg aattgtattc
     4981 caagtaagag cacttaattt cagagccgaa cgccatcgcc ttgtacccct ggtgcaggtt
     5041 gtgctgtccg gctcctttgt tctgaaggag cagaaggtat gtggactggc ttccaaaagc
     5101 caaagcgcct ggcttgcgat tttgaaacct taacggacaa atatggccgc ttttacgctc
     5161 agccgtttga acgtgggttt gggacgacta tcggcaacag cctgcgccgt gcgctgttat
     5221 catctatcga aggtgcagcg attacggctg tcaaaatcga aggggtgctc catgagtttt
     5281 cctcaattcc tggtgtcacc gaagatgcaa ccgatattat tttaaacctg aaacgaatcc
     5341 cgttcttaat gaccggtgcc ggagtcaaaa ccatccgcct tgaaagtaat actcccggcg
     5401 tggtgtattc tcgcgatatt gaggttggtt cagacatcga agttctggat ggttcaatcc
     5461 cgattgcgac ggtgagtgaa ggtggtcgcc tttcaatcga aatgcggctc aaaatgggac
     5521 gagggtatgt cccggctgac cgaaacacgg atgaagatct ctcggttggg tatattccga
     5581 ttgactcagt tcattcacca atcaaaaaag tcaattacca ggttgaggct gcccgcctcg
     5641 ggcaggatac tgactatgac aaattgacga ttgaagtgtg gaccaatggg agtatcaaac
     5701 cggacgacgc gattggttta gccgccaagc tgatcaaaga tcatatgacg atctttatca
     5761 attttgacga cactgaaccg gagatgaaag aaccagaaat cattgatcgt ccaaagcgga
     5821 ccgtcaacga atatctggat cgttcggttg aagagcttga gttgagcgtt cggtcctaca
     5881 actgcctgaa aaatgctgat attcgcacga ttcgggaact ggttcagaaa actgaacagg
     5941 aaatgttgaa aacaaagaat ttcggacgca agtctttaaa cgaaatcaag gaaatcatga
     6001 ccgcgatggg tctccgcctt ggtaccatct ttgatgaaga aggaaacatc gttgggtatc
     6061 gcgacgagta atttgggcaa actgtcgtga ggacagtttc cccattcacg tggaaacgac
     6121 tatgagacac ttaaacgcac accggaaatt ggggcgaacc tcagcacacc gtaaatcact
     6181 gttacgcaac ctcgccacct cgctcatcat tgaagagcgg ttagtcacca ctctgcccaa
     6241 agccaaagag ctgcgtccat ttgtcgaacg ggctattacc ctgggcaaac gcggtgattt
     6301 gcatgcccgc cgcctggcag ccagctactt tcatgctgga aaccagaacc ggattcccga
     6361 tggtggtcgg ggtcaccaac gctcaccacg caccgccggg gtgctggcgg tcagcaaact
     6421 gtttgatacg attgctgccc ggtttggtga tcgtccaggt ggctacaccc ggattgtcaa
     6481 acttggtcat cgtcgaggtg atggcgccga aatggcgc
//
LOCUS       DAOQDO010000041         6514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7012_length_6514_cov_3.249265, whole genome shotgun sequence.
ACCESSION   DAOQDO010000041 DAOQDO010000000
VERSION     DAOQDO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7012_length_6514_cov_3.249265"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..495)
                     /locus_tag="PKZ53_01270"
     CDS             complement(64..495)
                     /locus_tag="PKZ53_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39092.1"
                     /translation="MKSATHEDAQLILKLYELRREDVMRKARNYYLFLFFPSSVDDIR
                     AILTDRENPEPGAYFRQVTSYWEMAASLVNRGALDEDLFLDCNGEHLAVFAKVEPFLT
                     ELRQFFGPQYLSQLEQLIERMPDGKERIARFREMMKSLDPR"
     gene            complement(610..3936)
                     /locus_tag="PKZ53_01275"
     CDS             complement(610..3936)
                     /locus_tag="PKZ53_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39093.1"
                     /translation="MRLVLCGVVSFTWLTTLAAPPEPPTPSAEPPPLSDLAALGRPFF
                     RTYTDRDGLPQNTIQALAVDSRGYLWIGTQDGAACYNGRQWTTVRLPKEAASNFIQHI
                     CPAPDNRIWFTTDKDDVCCLNQQDGTWQYFSVAPPQSTTSTISAARVLPILPQNQRTL
                     VVATTNGEVLVFEQGQWHPLESIPKQTGDWVTAFGEATTPDGKPQIWIGTRNRRLYQN
                     SGSEWIWGGLPQSSSRVPILNISALVDQKGVTQLWVTIGRTIWHLDGGWTPIELPRSL
                     FGNQLIHIVTSLVTTDAQGQTILWVGTNAGLLRYHTGQWTLFDIRKGLPHNFVYQLLE
                     IKSGNSMPILFIGTLGGLSRLVEGKWLAIDSQAGLPNPFVLSILESVQPDGPPSFWFG
                     SFGGGRGGSLSKFENGEWTSFENHAVIPNNVGWALSEITAADGNKKIWYGSNAGLFEM
                     DGQEWHKIPTQLNPATGGVMSLSPVLNEPGQPEEVWVGLSNGLLHFYNGATTTYTTES
                     GLPSNTVTQLLITTSQNGQKVVWVGTTAGLARFENQQWTVYTRQNGLVYDHILSLCES
                     RPDASSHFLWIGTRGGVSRLDLSASTPLWKNFSETTIPALPNDTVYQIREDSHHRMYF
                     STNRGILRLTAQSPSLQDYTVTSFSLEDGLPSNECNRGASLVDHQGRLWFGTIAGAVV
                     FDPTKEILDTSPKPLFLERILVGGQPVTSQAIRSLRYTENNLSFEYALLSFFREGDTR
                     FQTQLVGLEASPTPWTADFKKEYLNLGEGQYCFQVWGKDYAGNVTGPVAIQFEIHPAP
                     WRSWPAYVVYVLAGTGAIFALFRYRLRVLQHRTQELEAKVAERTAQVVAQKNELAEAN
                     LKLRELDQIKVNFTAMLVHDLKSPLSVVKATLELFEIEESIQHSTLMPLVTASERSID
                     KILALINEVLEVFRSETQGITLRKSFIDTEKLLRECAEEARVAAVPHNISVTYRIEPN
                     LPSVFADPEKLERVFSNLFSNAIKFTQDGGSITLESRTVQGQGVETGITKLLITITDT
                     GEGIPAEDLPYLFDPYHQAQSRKAKLGIGLGLAIVKRIIAAHGGNISVRSQPGVGTSF
                     TIMLPIMTSSDRLQKVEIEE"
     gene            4239..5162
                     /gene="mdh"
                     /locus_tag="PKZ53_01280"
     CDS             4239..5162
                     /gene="mdh"
                     /locus_tag="PKZ53_01280"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNJ39094.1"
                     /translation="MGRKKVTVVGAGNVGATVAELIALKELADVVLIDIVEGLPQGKA
                     LDMNEMAPVEGYDALLTGSTSYEASANSDIVVITSGKPRRPGMSRDDLVEENKKIVAA
                     VTEQVAKYSPNAILVMVANPLDAMCTVAQRVSGFPRNRVIGMAGVLDSARMRFFLAEA
                     LNVSVENITAFVLGGHGDTMVPLPRYSTCAGIPIPELLPAETIEAIMNRTRNGGAEVV
                     KLLGTSAWYAPASASVEMVEAILKDKKKILPCAALLEGEYGYNGLFIGVPVKLGANGI
                     EQIIEISLTPEEKAALDKSAAAVQELVDLLK"
     gene            5408..6457
                     /locus_tag="PKZ53_01285"
     CDS             5408..6457
                     /locus_tag="PKZ53_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ39095.1"
                     /translation="MLPEAWKHLFENVWFTLVVAALTGILGEIGFRYFYKWFRDFTES
                     TETEIDNLLLARMHYPMRIAVWLGAVLVFLHWQPQFSTSLMRKVIDVVEVGLILYIIV
                     ETLETLGIDYLLVERRQVDIPWLLRDIGRVLIFSILGIVLIQNVFGADLTPFLATSTV
                     ISVVLGLALQSTLGNLFAGIALHLERPLRLGDWILYRTFEGKVVQMGWRSVTLQTFDG
                     DLVTVPNHAISTADVVNFYAPTKLHARKLRILVKHQVSPEMVETAVALAVEQVPQVLK
                     DQPIKVWLVEYSPVAIQYTVKFWIEDFAFHDDIESHLMKQFWHAFARLGLEHPLAVSV
                     LLPTTKDEWLGIKAE"
BASE COUNT         1499 a         1571 c         1774 g         1670 t
ORIGIN      
        1 cattctcaat tcttcattct caattcttca ttctcaattc ttcattctca attctccatt
       61 gtcttatcgc ggatccaggc ttttcatcat ttcacggaaa cgggcaatcc gttctttgcc
      121 atcgggcatc cgttcgatga gttgttccag ttgcgaaaga tattgagggc caaaaaactg
      181 gcgcaattcg gttaaaaacg gctcaacttt ggcaaaaacg gcaagatgtt cgccgttaca
      241 gtccaggaag aggtcttcgt ccagcgcgcc acggttcacc agtgatgccg ccatttccca
      301 ataactggtg acctgacgaa agtaagcgcc aggttccggg ttttcccgat ctgttaaaat
      361 tgcccgaatg tcatcaactg acgatgggaa gaacaaaaac agataatagt ttcgtgcctt
      421 gcgcatgacg tcttcacgcc gtaattcata gagtttcaaa atcaactgag catcttcgtg
      481 agttgctgat ttcataacta tccaattcct tccgttggtt ataaattgct aagtcagtgt
      541 tagaaaaagc tgaattgacc gctgactatt gcttcaattt ttcattctca cgtcttcatt
      601 cttcattttt tattcttcaa tctcaacctt ttgcagtcgg tctgatgagg tcataatcgg
      661 cagcataatt gtaaaactgg ttcccacgcc aggctgactt cggactgaaa tattgcctcc
      721 gtgggcagca atgatgcgtt tgacaatggc cagcccaagg ccaatgccga gtttggcttt
      781 tctggattgg gcctgatgat acgggtcaaa caaataggga aggtcttcgg cgggaatgcc
      841 ttcgccggtg tcagtaatgg tgatgaggag cttggtgatt ccggtttcga cgccttgccc
      901 ctgaactgtg cgtgactcaa gggtgattga cccgccatcc tgggtaaatt tgatggcatt
      961 ggaaaaaagg ttactgaaga cacgctccag tttttcagga tcagcaaaga cggatggtaa
     1021 attgggttcg atgcgatagg taacggaaat attgtggggt accgcggcca cacgagcttc
     1081 ttcggcacat tccctgagca gtttttcggt gtcaataaag gatttgcgga gcgtaatccc
     1141 ctgtgtttca gagcgaaaga cctcaaggac ttcgttgatg agcgccagta ttttgtcaat
     1201 gctgcgttct gaagccgtga ccagcggcat cagggtgctg tgctgaatgc tctcttcgat
     1261 ttcaaacagt tcaagcgttg ctttgaccac gctgagcgga gacttcaaat cgtggacgag
     1321 catggccgta aagttgactt tgatttgatc aagttccctg agtttgagat tggcttcggc
     1381 gagttcattt ttctgagcga cgacttgagc ggtgcgttcg gcaactttgg cttccagttc
     1441 ctgggttcgg tgctgtaaca cccgcaaccg atagcgaaac aaagcaaaga ttgcccctgt
     1501 tcctgccaac acgtagacca cataggctgg ccaggaccgc cagggcgccg ggtggatttc
     1561 aaactgaatc gcaactggtc ccgtgacatt accggcataa tcctttcccc acacctggaa
     1621 gcaatattgt ccttcgccga ggtttaagta ttctttcttg aaatcggctg tccagggggt
     1681 tggcgatgct tcaagcccga ccagttgggt ttggaatcgg gtgtcgccct cacgaaaaaa
     1741 tgaaagcagg gcatattcaa aggacaaatt gttttcagtg taccggagtg accgaattgc
     1801 ctgggatgtc accggttgcc caccgaccag aatgcgttcg aggaacaggg gtttaggcga
     1861 agtatctaga atttctttgg ttggatcaaa aacaaccgcg cccgcaattg tgccaaacca
     1921 gagccgtccc tgatggtcaa ccagcgaagc gccacgattg cactcgttgc ttggcaagcc
     1981 atcttcaagt gaaaacgatg taactgtata atcttgaagg gaaggtgatt gagctgttag
     2041 ccggaggatt ccccggttgg tcgaaaagta catccggtgg tgggaatctt cgcgaatctg
     2101 gtacaccgtg tcgtttggca gggccgggat cgtggtttca gaaaagttct tccagagtgg
     2161 ggtcgaagcc gagagatcaa gtcgggaaac accaccgcga gtaccaatcc agagaaagtg
     2221 cgaactggca tccgggcgtg attcgcacag gctcaggata tgatcataca ccaggccgtt
     2281 ttggcgggta tacactgtcc actgctggtt ttcaaagcgt gcgagcccgg ctgttgtccc
     2341 tacccagaca actttctggc cattctggct ggttgtgatc agcaactggg tgacggtatt
     2401 gctggggagg cccgattctg tggtgtaggt cgtggtggca ccgttgtaaa agtggaggag
     2461 cccgttgctc aagccaaccc agacctcttc gggttgcccc ggttcattca agaccggaga
     2521 cagggacatg accccgccgg ttgccgggtt gagctgggtc gggattttat gccattcctg
     2581 gccgtccatc tcaaacaaac cggcatttga tccataccag attttcttgt ttccatcagc
     2641 cgccgtgatt tcgcttaaag cccaaccgac attattggga atgacggcat ggttttcaaa
     2701 actcgtccac tctccatttt caaatttcga gagtgatcca ccgcgcccgc ctccaaacga
     2761 gccaaaccag aatgatggtg ggccgtcagg ctgtacgctt tcgaggatgg agagcacaaa
     2821 tggattcgga agcccggcct ggctatcaat tgcgagccat tttccttcaa ccagccggga
     2881 gagcccgcca agcgtaccaa taaagagaat cggcatggaa ttcccggatt tgatctccaa
     2941 aagctggtac acaaagttat ggggcaatcc tttgcggatg tcgaacaggg tccattgtcc
     3001 agtgtggtat ctcaacaggc cagcattggt tccaacccag agaatggtct gtccctgcgc
     3061 atcagtggtc accagagacg tcacaatatg gatcagttga tttccaaaca gggatcgggg
     3121 caactcaatc ggtgtccatc ctccatccag atgccagatg gttctgccaa tggtcaccca
     3181 taattgtgtc accccttttt gatcaacaag tgcggagata ttcaaaatcg gtacccggga
     3241 agaggactgc gggagcccgc cccagatcca ctccgagcct gaattttgat agagccgacg
     3301 atttcgtgtt ccgatccaga tctgaggttt tccatccgga gtcgttgctt caccgaaggc
     3361 agtcacccag tcacctgttt gtttggggat gctttccagc gggtgccatt gcccttgctc
     3421 aaaaacaaga acttcaccgt ttgtggtggc aacgacaagt gttcgctgat tttgggggag
     3481 aatcggcaag acccgtgcgg cactgatcgt ggaagttgtg gactgtggcg gcgcaactga
     3541 aaaatactgc caggtgccat cctgctgatt gagacagcaa acatcatctt tgtcggtggt
     3601 aaaccagatc cgattgtcag gtgcggggca aatatgctgg ataaaattgg aggcggcctc
     3661 ttttgggagg cgaaccgtcg tccattgacg gccattgtaa caggcagcgc catcctgggt
     3721 gccgatccag agatagccac gcgaatcaac ggccagtgcc tgaatggtat tttggggtaa
     3781 cccatcacga tctgtgtagg tccgaaaaaa aggtcgtccg agcgcagcca gatcggagag
     3841 aggaggtggt tccgctgatg gtgttggggg ttctggtggc gcggccagcg ttgttagcca
     3901 ggtgaagcta accacgccac acagcaccaa tcgcatgatg cagatccgga ttcggggtct
     3961 catgaggatg agcggaagtc ggaaaatgga aagttgaacc ggggcttgaa gggagaagct
     4021 ctcttcattt acctgaaacc ctggtcagac acaagaggga cgaaggtttt gatgaaagat
     4081 gctgttgctc ctgggtggtt ttcaaggccg gaaacagcac cgaacttgtt gccaaatttc
     4141 gacttgccac gtatgctttc gcgcgcattt tattcgtcct tttatgcctc ctggacgaat
     4201 ccatttttca tacatgtcag aacagaaggg aattttttat gggtcgtaaa aaagtcactg
     4261 ttgtcggtgc cggcaacgtt ggtgcaactg ttgccgaact gattgcattg aaagaacttg
     4321 cagatgttgt gttaattgat atcgtcgaag ggttgccaca gggaaaagcc ctggatatga
     4381 atgaaatggc accggtcgaa ggctatgatg cgctgttgac tggatcaact tcatatgagg
     4441 cgagcgccaa ttccgatatc gtcgtcatca cctctgggaa accgcgtcgg cccggcatga
     4501 gccgtgacga tctggtcgaa gaaaacaaaa agattgtggc ggcagtcacc gagcaggttg
     4561 ccaaatattc acccaatgcc attttggtga tggttgctaa tccgctcgat gccatgtgta
     4621 ccgtggcgca acgggtttct ggctttccac gcaatcgcgt gatcgggatg gctggtgtgc
     4681 tcgactcagc ccgcatgcgg tttttcctgg ccgaagccct caacgtgtcg gttgaaaaca
     4741 tcacggcgtt tgtgctgggc ggtcacggcg acacgatggt gccgttgcca cgttattcaa
     4801 cctgcgcggg gattccgatt ccagagttgc ttccagccga aaccatcgaa gcgattatga
     4861 accggactcg caacggtggc gccgaagtcg tcaagttgct cggcacctcg gcctggtatg
     4921 ctccggcgtc agcttcggtt gaaatggtgg aagccattct caaggacaag aagaaaattc
     4981 tgccttgtgc ggccctgctt gaaggcgagt atggctacaa cgggctgttt attggcgttc
     5041 cggtcaaact gggcgccaat ggaatcgagc agatcattga aatcagcctg actccggaag
     5101 aaaaagccgc gctggataaa tcagccgcgg cggttcagga actggttgat ttgttgaagt
     5161 agtcgcgtga aaaaaaggga aagtcaggtc aagagatctg gctttccctt atgatttttc
     5221 ccaggattcc catttctggc tgttcctcaa accagttgat ttcccaccca tccccacggg
     5281 agtcagtacc attctctgtc attgatgtgc cttcatttcc acaggtttgg actctacaac
     5341 ctgtctgctg gaagtgtctg tgccggtgat gtgccagaag caatgatttg aagctacgga
     5401 gtcaaagatg ctacctgaag cctggaaaca cctctttgag aatgtatggt ttacccttgt
     5461 ggttgcagcg ttgaccggga ttttggggga aatcggattc cgttattttt ataaatggtt
     5521 tcgggatttt actgaaagta ctgaaaccga gattgataac ttgttgctgg ctcggatgca
     5581 ctatccgatg cggattgccg tctggttggg ggccgtgctg gtgtttttgc actggcagcc
     5641 acaattttca acctcactga tgcgcaaagt gattgatgtg gttgaggttg gactcatcct
     5701 ctatatcatc gtcgagacac tggaaacact cgggattgat tatcttctgg tggagcggcg
     5761 gcaggttgat attccctggc tattgcgcga catcgggcga gtcttaattt ttagcattct
     5821 ggggattgtg ctcattcaga atgtttttgg ggcagattta accccatttt tggcgacttc
     5881 gaccgtcatc agcgtggttt tagggttggc acttcagagt accctgggca atctgtttgc
     5941 cggtattgcg cttcacctgg agcgtccgct tcgactggga gattggatcc tctaccgcac
     6001 ctttgaaggg aaagtggtcc agatgggctg gcgttccgtc actttgcaaa ccttcgatgg
     6061 ggatctggtt actgtgccga atcacgccat ttcaacggct gatgtggtga acttttacgc
     6121 gccaaccaaa ttgcatgctc gaaagcttcg aattttggta aagcaccagg tgtcacccga
     6181 aatggttgag accgctgttg cactggccgt cgagcaggtg ccgcaagttc tcaaggacca
     6241 gcccatcaag gtctggctgg ttgaatactc accggtagca attcagtaca ccgtcaaatt
     6301 ctggatcgag gattttgcct ttcacgacga catcgagagc catttaatga agcagttctg
     6361 gcatgccttt gccagacttg gacttgagca cccgctggcg gtttcggtcc tgctgcccac
     6421 caccaaggac gagtggctgg gaatcaaggc agaatgaaga acgggttccg ggttccgggt
     6481 tccgggttcc gggttccggg ttccgggttc cggg
//
LOCUS       DAOQDO010000042         6513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7014_length_6513_cov_6.014246, whole genome shotgun sequence.
ACCESSION   DAOQDO010000042 DAOQDO010000000
VERSION     DAOQDO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_6513_cov_6.014246"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKZ53_01290"
     CDS             <1..456
                     /locus_tag="PKZ53_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNJ39096.1"
                     /translation="ASISMDQGRIAVSNMFNVGDVKQFAKVYPFAIYTVPEVSMVGLT
                     EEAAQKEGLDFCTGQARYGDMPRGKIMGTEDGLLKILFTREDLIIRGVHIMGNIASEL
                     IHYGLTLVQDQKTLHDVIATIFNYPTLHDLYKYACYDGLGNLAGRKLKD"
     gene            691..2004
                     /locus_tag="PKZ53_01295"
     CDS             691..2004
                     /locus_tag="PKZ53_01295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNJ39097.1"
                     /translation="MASRKRFSRHHTKKQSSPAASSPTAPQLTRTETVTIEKLIYGGE
                     GLARLADGRTLFVPGVVSGEKVEVEITELRRTFARGRLTQVIEASPDRIEPPCPHYGR
                     CGGCHLQHLSYPAQIAAKSEFIREALQRIGGLNWSEPIPIRTGPEWSYRNRTQFKLHF
                     NPHTKQSEAGFFAAHSHRIEPITECRVLDPSLNQVLARLAHLPATHISATIVTLDLAT
                     DAAAGQDSSESASISASLPFVGLSTRPIVRQVAGFQYRFDTECFFQVNSFLLDTLVKE
                     VLSSLTSSPEAEAIAVDLYAGVGLLTLPLAHRFHRVFAAEAAPHTAHFAEINLKANQI
                     PNVTFQRLSTEEWLIEHLDLVDQVAFAVVDPPRAGLSPEVVDGLINLRPQRLVYVSCD
                     PSTLARDVRRLSTAGFVIETITGIDFFPQTYHIETVVQMRWEGFN"
     gene            2416..2856
                     /locus_tag="PKZ53_01300"
     CDS             2416..2856
                     /locus_tag="PKZ53_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNJ39098.1"
                     /translation="MLRGNHTARIDDKGRLKIPTPFRRLLEERYGLLLYITSLTGECA
                     RIYPMPEWTAIENRLLALPVMDPARRKFLDRTNYYGQEAEMDTQGRVLIPYQLRSKAE
                     MLGDVAVLGYLNYLEVWSLERFEARLAADPFTDDDAAALARLGI"
     gene            2959..3978
                     /gene="rsmH"
                     /locus_tag="PKZ53_01305"
     CDS             2959..3978
                     /gene="rsmH"
                     /locus_tag="PKZ53_01305"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNJ39099.1"
                     /translation="MIFEKSAPLIQHLPVMVDEILTLLEPERGGWIVDGTLGLGGHTE
                     AFLKQSETVRVLAIDRDTEALALARQRLAPFADRVIFVHGDFRDLRHILVQHHLTQVF
                     AILVDLGVSSWQLGQAHRGFSFQLNGPLDMRMDQTKSVTAAELVNTLSAEELANVIFE
                     YGEEPASRRIARRIVNERVKHPIETTEQLAELVRRAVPFSPKQFRIDPATRTFQALRI
                     AVNRELEHLDQFVTDAIDHLLPDGKLAVITFHSLEDRLIKRAFRVEAGMENPVTDRRS
                     LWLTPHHERTVPPKRISLVTKKPVVASEAEIDRNPRARSAKLRVCQRVSSFDSEALFS
                     PPLPA"
     gene            4125..4460
                     /gene="ftsL"
                     /locus_tag="PKZ53_01310"
     CDS             4125..4460
                     /gene="ftsL"
                     /locus_tag="PKZ53_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNJ39100.1"
                     /translation="MQRYRLQSSIQNTCPKRALDRTWFRQWGLTIILGTSVACGFVYV
                     SWQHVKAVEIGYETEHLKQQIQQLDQDQRKLELERQRLLSPSVLENRARRQGMGLPQA
                     NQTIVIEQR"
     gene            4540..>6513
                     /locus_tag="PKZ53_01315"
     CDS             4540..>6513
                     /locus_tag="PKZ53_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNJ39101.1"
                     /translation="MALRETIKQKTTRKKLVAVTSPQSLSPARLSVIAVVLLIWMGLI
                     IARLVRYQVHEHQAMCERAEQQQQRVVETTPRRGTITDRNGYQLATSTEVASVYVSPG
                     QVPKPAPHVDELAKILEMPRDTVMQRLTSSKVFVPLKRKITDDQAKAITQLDIPGIQF
                     VTETKRSYPQGELASTVLGYVNFEEQGAAGVELEFDKHLRGTSGKVLVESDAKGRPFH
                     RNQQTSEVGQSLTLTVDQRIQYKTEQILKETVASTRAKGMMAVVMDPTTGDILALATT
                     PTFDPNSRAENEEELRARRNRIAEEAYEPGSVFKIVTYSAALEEKVLRPTDAINCQGG
                     SITIAGHTVRDGGGYGMLTATEAISVSSNVAAIKTGRQLGKERLLKYVERFGFGQPTD
                     AGFPAESAGSIGGVNRWSDLSYASLPMGYEVQVTPLQLAGAIGALANDGVWVQPHIVR
                     NILSTTGDVIFDTEPKTRRVVSVKTAQEMRAMLRNVVERGTAKKAKLSGYSAGGKTGT
                     AKKFDHKIGRYSEVKYFASFIGLAPVEKPKVVIAVVVDEPPFGAHHGGDAAAPVFKRI
                     AEMVLPIVGARPDVPFDPQDALLAADQNLPDEPDPFDLPGKPIAAPASATEAPPADVV
                     LTSANQAKSVSHPSSAATSPVRNHTPLVTVNAPS"
BASE COUNT         1599 a         1803 c         1615 g         1496 t
ORIGIN      
        1 gccagcatca gcatggacca gggtcgcatc gccgtcagca atatgtttaa tgtcggtgat
       61 gtcaaacaat ttgccaaagt ttaccccttt gccatctata ccgtccccga agtctcaatg
      121 gtcggactca ccgaagaagc cgcccaaaaa gaaggactcg atttctgtac cggacaggca
      181 cgctatggcg acatgccgcg tggcaagatt atgggcaccg aagatggctt gctgaaaatc
      241 ctgtttaccc gcgaagatct catcatccgt ggggtacaca tcatgggcaa tattgcctcg
      301 gaactcattc actatggatt gacactggtt caggatcaaa aaacgctcca cgatgtgatt
      361 gcaaccattt ttaactaccc gacgctccac gatttataca aatatgcctg ctatgacgga
      421 cttggaaacc tggctggccg aaaactcaag gattaaggtt gggttccggg ttccgggttc
      481 cgggttccgg gttccgggtt ccgggttccg ggttccgggt tttcgaagtt atgtcccttt
      541 cgtctttttc ccaaacccca aatcctgaca agatgaccga ctgacaaggt aacagggtga
      601 ttttttcatc cctcatcctt catccctcat ccctttcgaa aaccctgaac cctgaaccct
      661 gaaccctgaa tcctgaaccc gagatttccc atggcttccc gaaaacgctt ctcacgacat
      721 cacaccaaaa aacaatcatc cccagcggcc agctcaccca ccgcccctca attgaccaga
      781 accgaaactg tcactatcga aaaattgatc tatggcggtg aggggctggc ccgactggcc
      841 gatggccgaa ccttgtttgt tccaggtgtg gtcagcggtg aaaaagttga agtcgaaatc
      901 actgaattgc gccgcacgtt tgctcgggga cggctcacgc aggtcatcga agcctcacca
      961 gaccgcatcg agccaccctg tccgcactat ggccgctgtg gaggatgcca cctgcagcat
     1021 ctttcttatc cggcccaaat cgcagctaaa tcagagttta ttcgtgaagc gcttcagcga
     1081 atcggcggtc ttaactggtc agagccgatt ccgattcgaa ccggccccga atggtcctac
     1141 cgaaatcgga cccagttcaa acttcatttc aatccacata ccaaacagtc cgaagccggc
     1201 ttttttgccg ctcattccca ccgcattgaa ccgatcaccg aatgccgggt tctggatcca
     1261 tcgttaaatc aggtgttagc gcgcctggca catcttcctg caacccatat ctcagcaacc
     1321 atcgtgacat tagatctggc aacggatgcg gcggcggggc aagactcatc cgaatcagct
     1381 tcgatttcag catcattgcc atttgtcgga ctctctacca gacccatcgt gcgccaggta
     1441 gccggttttc agtatcggtt tgataccgaa tgtttttttc aggtcaattc atttttactc
     1501 gacacactgg tgaaagaagt tctttccagt ctcaccagtt cgcctgaggc tgaggccatt
     1561 gccgtggacc tgtatgccgg agtgggcttg ttgaccctgc cgctggccca tcggtttcac
     1621 cgtgtatttg cggctgaagc agcgccgcat accgctcatt ttgccgaaat caacctcaag
     1681 gccaatcaga ttcccaacgt gacctttcag cggttatcaa ctgaagaatg gttgatcgaa
     1741 catcttgatc tggttgatca ggttgccttt gccgtggtgg acccaccccg cgccgggtta
     1801 agccccgaag tcgtggacgg tctgatcaat cttcgcccgc aacgtcttgt ctatgtctcc
     1861 tgtgaccctt ccacccttgc ccgtgatgtg cgccgactga gcacggctgg atttgtgatt
     1921 gagaccatca ccggaatcga ttttttcccc caaacctacc atattgaaac cgttgtccag
     1981 atgcgctggg aaggcttcaa ctaaaacttc aaatagaaat ccataaaacc aactaaataa
     2041 ataagttatc aagatgaatt taaactgcat cttgtgactt ttttccagcc tttgcgcttg
     2101 acacagtgtt tttcagtaac gtaatattcg atcctgtgga aaaaagtggc caaaagtcgc
     2161 tttttttagc gttttgccta tcaaaacctc aagttacacg ttaagccctt cacatttgat
     2221 tccccgaacc tttgctgtga ccagtagctg caatggacct ccagcgtaag ttcttttgta
     2281 ttcaaagagt tgggctacgt tctgtgccag gatgtgttgt gagtttggtg acacatccca
     2341 caccagaggc tcgacaggca caaccatcgc cgcagaaacg atgcttgacg gtgagcacag
     2401 agcgaggata agcccatgct gcgagggaat catacagccc gcattgatga caaagggcgg
     2461 ttaaagattc caactccctt ccggcgattg cttgaagaac ggtatggtct gctgctctat
     2521 atcaccagcc tgactggtga atgcgctcgc atctatccga tgccggaatg gaccgcgatt
     2581 gaaaaccgac tcctggcgct tccggtgatg gatccggccc gccgcaagtt tctggatcga
     2641 accaactatt acggtcagga agctgaaatg gacacccagg gacgggtttt gattccgtac
     2701 cagttgcggt caaaagctga aatgctgggt gacgtcgcgg tgcttgggta tctgaattac
     2761 cttgaggtct ggagtctgga acggtttgaa gctcgactgg cagccgatcc atttaccgat
     2821 gacgacgcgg cggcattggc cagactcgga atctagccac ggcacaatgc caatcatcaa
     2881 gtatgaatca cgagacaggt aattccttat agtgaaagaa gatacactct tcatcctttc
     2941 tgctttctgt aaacggcaat gatatttgag aaatcagctc ctttgataca acacctgcct
     3001 gttatggtgg atgagatcct gacgttgctg gaacctgagc gcggtgggtg gatcgtggat
     3061 ggaacgcttg gtctgggcgg ccacaccgaa gcatttctga aacagtcgga aacggttcgt
     3121 gtgctggcaa ttgatcgtga taccgaagcg ctggcactgg ctcgccagcg gctggccccg
     3181 tttgcagacc gtgtcatctt tgttcacgga gattttcgcg atctgcgcca catcctggtc
     3241 caacaccatc tcacgcaggt ttttgctatt ttggttgatc ttggggtttc ctcctggcag
     3301 cttggtcagg cccatcgggg gtttagcttt cagctcaatg gaccactcga tatgcgcatg
     3361 gatcaaacca aatctgtgac cgcagcagaa ctggtgaaca cgctttcggc tgaggaactg
     3421 gccaacgtga tctttgaata tggtgaagaa ccggcttccc ggcggattgc ccgtcgcatt
     3481 gtcaatgaac gtgtcaaaca cccgattgaa acgacagagc aactggctga actcgtccga
     3541 cgtgcagttc ccttttcacc gaaacagttt cgaattgatc cagcgacccg cacctttcag
     3601 gccctgcgga ttgcggtcaa tcgtgaactc gaacacctcg accagtttgt caccgatgcc
     3661 attgatcatc tacttccaga cggcaaactg gcagtcatca cgtttcattc actggaagac
     3721 cgactgatca agcgtgcttt tcgggttgaa gccggaatgg aaaacccagt cactgatcga
     3781 cgctctctct ggctcacccc gcatcacgaa cggaccgttc cccccaaacg gatttcgctg
     3841 gtgaccaaaa agccggttgt cgcttcagaa gctgaaattg accggaaccc tcgtgcccgc
     3901 agtgcaaaac ttcgtgtttg tcagcgagtc agttcgttcg attccgaggc tctcttctca
     3961 ccccctttac cagcgtagcg gatcgtctct ggccaggcag aattggccaa tcaaccagaa
     4021 cccctgacag ccgtcacgat ccagccagtc aggaaaccat ccgaattttg ttcaacatca
     4081 agcagggaca cttcattcac catgaaagga acctgagacc gggtatgcaa cgctatcgac
     4141 tgcaatcttc aatccagaat acctgcccca aacgcgcact tgaccgcacc tggtttcgtc
     4201 agtggggact caccatcatc ctcgggacgt cagttgcctg cgggtttgtg tatgtgtcgt
     4261 ggcaacacgt caaagcggtt gagattggat atgaaactga acacctgaaa caacaaatcc
     4321 agcaacttga ccaggaccag cgcaaacttg agcttgaacg gcaacgattg ttgtcaccca
     4381 gcgtgcttga aaatcgcgcc cgccgccagg gaatggggct tccacaggcc aaccaaacaa
     4441 ttgtaattga acagcgctaa ccagtctcag accaggccgg tgcagtcagg cacggctggg
     4501 gactggcagc gacaatttcc caaaaccagt tgagtgagca tggcacttcg cgaaacgatc
     4561 aaacaaaaaa caacccggaa aaaactggtg gcagtcactt cgccccaatc gctgtcgcca
     4621 gcccgtctga gcgtgattgc ggtggtgttg ctgatctgga tgggtttgat catcgccagg
     4681 ctggtcagat atcaggtcca cgaacatcag gcgatgtgcg aacgagcaga acaacaacaa
     4741 caacgcgtgg ttgaaaccac gccccgccgc ggcaccatca ccgaccgcaa tggataccag
     4801 ttggcgacca gtaccgaagt tgcttcggtc tatgtctcac cggggcaggt gccaaagcca
     4861 gcacctcacg ttgatgaact cgccaaaatc cttgagatgc cgcgcgatac cgtgatgcaa
     4921 cgcctcacat catcaaaggt ttttgtgccg ctcaaacgca aaatcactga tgatcaggcc
     4981 aaagcgatta cgcagcttga tatcccaggc attcaatttg tcacagaaac caaacgcagc
     5041 tatccgcaag gcgaactggc gtcaaccgtg cttggctatg tgaattttga ggagcaaggc
     5101 gccgccggcg ttgaactgga atttgataaa cacttgcgcg gaacatccgg aaaagttctg
     5161 gttgagtccg atgccaaagg ccgtcccttt catcgcaacc agcaaaccag cgaagtcggt
     5221 caaagcctga cgttgaccgt ggatcaacgc attcagtaca aaacggagca aatcctcaag
     5281 gaaacggtgg cctcaacccg agccaaaggc atgatggcag tggtaatgga tccaaccact
     5341 ggtgatattc tggcgctggc aaccacacca acttttgatc ctaattcccg ggcagaaaat
     5401 gaagaggaat tgcgggcacg ccgcaaccgg attgcagaag aagcctatga accgggatcg
     5461 gtttttaaaa ttgtgaccta ctcggcagca cttgaggaaa aagttcttcg cccaaccgat
     5521 gcgattaact gtcagggcgg ctcaatcacg attgcaggtc ataccgtccg ggatggcggt
     5581 ggctatggaa tgctcacagc cacagaagcc atcagtgttt caagcaatgt ggccgcaatc
     5641 aaaaccggac gtcagcttgg aaaagaacgc ctgttgaaat atgttgagcg gtttgggttt
     5701 ggacaaccca ccgatgctgg atttccagcc gaatcagccg gatcaattgg tggtgtcaac
     5761 cggtggtcag atttgtcata cgcttcgctt ccgatgggat atgaagtgca ggtcaccccg
     5821 ctgcaactcg ctggcgccat tggggcgctg gccaatgacg gtgtttgggt tcagccgcac
     5881 attgtccgca atatcctctc aaccactggc gatgtgattt ttgataccga gccaaaaact
     5941 cgccgggttg tcagcgtcaa aacagcccag gaaatgcgcg ccatgctgcg caatgtggtg
     6001 gagcgcggga ccgccaaaaa agccaaattg agtggctact cagccggtgg aaaaaccgga
     6061 actgccaaaa aatttgacca caaaatcgga cgctattccg aagtcaaata ctttgcttcc
     6121 ttcattggat tggcaccagt tgaaaaacca aaagttgtga ttgccgtggt ggttgacgaa
     6181 cctccttttg gcgcgcatca cggcggcgat gcggcagcgc cagtgtttaa acggattgcg
     6241 gaaatggtgc ttccaatcgt aggcgccaga ccagacgttc cttttgaccc gcaagatgcg
     6301 ctgctggcag ccgatcaaaa tctaccggat gaacccgacc catttgattt gccaggaaaa
     6361 cccattgcgg caccggcctc agccaccgaa gctccacccg ccgatgtggt cttgaccagc
     6421 gccaatcagg ccaaatctgt ttcccatccg tcatcggcag cgacgtcacc tgtccgcaac
     6481 catactccgc tggtgacggt caatgcccca agc
//
LOCUS       DAOQDO010000043         6459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7121_length_6459_cov_2.901780, whole genome shotgun sequence.
ACCESSION   DAOQDO010000043 DAOQDO010000000
VERSION     DAOQDO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_6459_cov_2.901780"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..1662)
                     /locus_tag="PKZ53_01320"
     CDS             complement(370..1662)
                     /locus_tag="PKZ53_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ39102.1"
                     /translation="MANLLFVDDDETVLALLESATDPRHSICSCTSAEQALTQAQLRL
                     FDVAVLDVHLPGMSGLNLIPHLLRLCPGISIFVMTGAGTIEIAVDAMKRGAQDFLAKP
                     ISITHLQNLIEVALSRKQTGAKTESGEAVAAESKTQMIATAPKMQHLLAQVEAIAPYN
                     TTVLVTGETGSGKELLVRAIHAKSPRARRPFVALNCAAIPEHLLEDELFGHVKGAYTG
                     AIQPRDGRFEQANGGTLFLDEIGDMSLPLQSKLLRVLQERTFEKLGSARSVKVDVRVV
                     AATSANLEKRIEEGQFRADLYYRLNVMRLSLPPLRDRREDIRPMANFFLSRFCESVGL
                     PTKTIDDSVWPVLTSYDWAGNVRQLQNAMERAAALSGAATTILIRDLPDEIQRTYVAA
                     TPPVRTYAPAPEIQSPVNFEAGIDFEAVVSSFERDLLT"
     gene            1941..4958
                     /locus_tag="PKZ53_01325"
     CDS             1941..4958
                     /locus_tag="PKZ53_01325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ39103.1"
                     /translation="MNVLIFDFAPSSQVSAIRSALEQVWTEATVVLVSTPDQIEPVLA
                     EFSPDLAIYMAHSQTVETLSEIHYLLHTLSSGKLLCLGEVHHIKEAELILFPVQIGWL
                     PCGPPQSTAQAIVQWFQAQSGIDLAHSLQQTLLESEKRYRIVSEISSDFAFSLRQDTD
                     GRLKFEWLTAAFQRITGYSQEEIDNQGGWTSMVHPDDLALALQHLSRLNSGHSDIREY
                     RIITKAGAIRWLRTYSRPIIDPQTGLVTQIYGASQDVTEVNSGRLFESLMHQLDQMVL
                     QELPLEDIVSYACTRLTTLFSYSVAWVGLKEQTGSISVKGYSGLWARDCLSQLPLVWE
                     GTPETLGLAGLAITSLTPQIARPTDLHLWHWRKAGRYGVRSAIALPLIARGQTIGVIN
                     IYANQSDAFRQPYLSWLEALAARIAIALLISIDRLQLRLQSAALESAANAIIITNELG
                     NIEWVNPAFTRFSGYTAAEVAGKNPRILRSEEHPRAFFEKFWETIQSGQIWHGELINR
                     RKDGALYTVRQTVIPLLDQQNTITHFVAIQEDITQQREEEARMVKMAYYDPLTNLPNR
                     ILFQDRLRQALLQGRRNDWQVGVLFVDLDRFKRINDTLGHDAGDQLITEVANRLSASV
                     RRSDSVARLGGDEFTVILPDLPDTDGALIVAQKIITALSEPILLKGHQVQITASVGIA
                     LFPDHATDAEGLVTRADIAMYEAKQRGKNTFALYEPGMDLHSDSAFILEHSLPKALQS
                     NQFMVDYQPIFNVRSGVPVGVEAGLYWKHKDRGPLAFAQFVDLAEQSGTLKQIMNWYL
                     QTAIHQFQTWADQVSPEFRFVLPMPVASLCEPQTIDCLKNLVQELGLSFSWLEIALQE
                     AELVSTTGTVQLIIDQLRDEGIQFSLRGFGVGNLPLATLHRFNFHLVNLDPALMSQLG
                     ESESSATLVSGLTEFLHHLGIKVGAEGVEAECQIKLLAQFGCDELKGKGIEWWFNAPN
                     FDLICGKSATAGESSFVAVKSFESLDNPLRKIS"
     gene            5025..>6459
                     /locus_tag="PKZ53_01330"
     CDS             5025..>6459
                     /locus_tag="PKZ53_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ39104.1"
                     /translation="MHSHPDEQSSYPGPKRSSSQTGYQKFRFLADHLAQPVIEWDALL
                     IITRMNAAAQTSFEQSGTDWVGRSLTELMSEPVELLKTRLSTTGSGWMEYRQGAGCKG
                     HWSWVALKSATSESEGGIAIITSSAVDPEADLKPEPARFVPTRELCTVLDAIPANVAL
                     VDRFGIIQVVNEPWKNYARANNFKDDTLGLGWNYLEVCRQSQGSSMVAETAYVLLKSV
                     LDEQSGPEELEYPCHSLSAKQWFSMIITPIKLEGQPSALVIHTDITEQKLAEQALRKS
                     EARYRSLIAATAQSVWTTQANGQVVGDQPSWRALTGQSVADIQGWGWLEAIHPADREQ
                     LQQVWIYALETKSLLSVEHRVRIASGEYRSFAVRAVPMTDEAGKVDEWIGTHTDITDQ
                     KRALDELKKAETQLRHSQRMEAVGRLAGGVAHDFNNLLMVISGYADLVANKLGKTHAL
                     YRFLEEIRQASRSASGGTRQLLAFSRKQ"
BASE COUNT         1544 a         1679 c         1682 g         1554 t
ORIGIN      
        1 atcggtgagc aactaggcaa tgagtgccag ctccaggagt catagaaccc ggacgtaact
       61 caacgcccag tcagtggcct caaaggttgc agctcctggt tgccaaaaaa ttcaaaaaga
      121 aacccaaaga gacggcagta cactggttgc aaatgtgctg ccgtctcttt ttggtaagtg
      181 attgatatat ttcgtgtatt gtgtgatttc aatatctgga aattttcagc gaagcaccga
      241 tcaaagggtt cgatacaatt attgaccacc accgatggaa tactttttca acttttccaa
      301 aaaagtggtg cgcttcatgt tgagaagtct ggcggcttgc aatttattgc caccagtttt
      361 ttccagggct caggtcagca ggtcgcgttc aaaacttgag accacagcct caaagtcaat
      421 tccggcttca aaattgacgg gtgattgtat ctcaggggcc ggtgcgtagg ttcgaaccgg
      481 gggggttgcc gcaacatagg ttcgctgaat ctcatcaggt aaatcccgga ttaaaattgt
      541 ggttgccgca ccactgaggg cagccgcccg ctccattgca ttttggagct gacggacgtt
      601 tcccgcccaa tcataactgg tcaggactgg ccagactgag tcgtcaattg tttttgtcgg
      661 cagcccgacc gattcacaaa accgactgag aaagaaattg gccattgggc ggatgtcttc
      721 gcggcggtcc cgaagcggag gcagcgagag tcgcatcaca ttgagccgat agtacaaatc
      781 cgcccgaaat tgaccttctt caatccgttt ttcaagattg gcactggtgg cggccaccac
      841 tcgaacatca accttgacgg atctggccga gccgagcttc tcaaacgttc gttcctgcaa
      901 cactcgcaaa agtttggatt gcagcggcag gctcatgtcc ccaatttcgt ctaaaaacaa
      961 cgtcccgcca ttggcttgtt caaagcgtcc atcacggggt tgaatcgcac ccgtgtaggc
     1021 gcctttgacg tggccaaaga gttcatcttc cagcagatgt tctggaatcg ccgcacagtt
     1081 gagggccaca aacggacgcc gggctcgtgg gcttttggca tgaatggccc ggaccagcaa
     1141 ttcttttccc gacccggttt caccggtcac cagcactgtg gtgttatagg gggcaatggc
     1201 ttcgacctgg gccagcaaat gttgcatttt gggtgcggtt gcaatcattt gggtcttgct
     1261 ctcagcggca acggcttcac ccgattcagt tttggcaccc gtttgttttc gagaaagcgc
     1321 aacttcaatc aaattctgaa ggtgggtaat cgaaatcggt ttggccagga aatcctgagc
     1381 cccacgtttc atggcgtcaa cggcaatctc aatcgtaccg gcgccggtca tgacaaaaat
     1441 agaaatccct gggcagagcc gcagcaaatg gggaatcagg ttaagtcctg acatccctgg
     1501 caaatggaca tccaaaaccg ccacgtcaaa gagccggagc tgcgcctggg tcagggcctg
     1561 ttcggcgctg gtacagctac aaatcgaatg ccgcgggtct gttgcgcttt ccagtaatgc
     1621 taataccgtt tcatcgtcat ccacaaaaag cagatttgcc acaatcggtt cctcaacctt
     1681 ccagcatctg tttgccatgt cagaaacctg acaacagttg atacgccagg tgagttttcg
     1741 tcaggcagga ctttaaattg acgtttgagt aggcgatcca acaggcaaag tactgcactt
     1801 ctacatcctg gacaccgatt cgtcaatatc attcgattgt aattcgtgga atggtatttg
     1861 cacttcctgg ggcaagattt ggttgctggc agttcagcct ggctgaattt gccgaaatcg
     1921 cacattgcgg aggtatcagc atgaacgttc ttatttttga ttttgcccca tcatctcaag
     1981 tgtctgccat ccgcagtgct ctcgaacagg tctggaccga ggcaacggtg gtgctggtca
     2041 gtacgcctga tcaaattgaa ccggtactgg ctgagttcag ccctgatctg gccatctata
     2101 tggctcattc tcaaaccgtt gagacacttt ctgagattca ctatctgctc cataccctat
     2161 cgtctggcaa gctgctgtgt ctgggggagg ttcatcacat caaagaagct gaactgatcc
     2221 tgtttccagt tcaaattgga tggcttccct gtggtccgcc acaatcaacg gcccaggcga
     2281 ttgtccagtg gtttcaagcc cagtcaggaa tagaccttgc ccattccctt caacaaacat
     2341 tgttagagtc ggaaaaacga tatcggatcg tctctgaaat ttcctcggat tttgcttttt
     2401 cgcttcgaca agataccgat ggccggctga aatttgagtg gctcacggct gcctttcaac
     2461 gcattacggg ctatagccag gaggaaattg acaaccaggg tggatggacc agcatggtcc
     2521 acccggatga cctggcactg gctttgcaac atctctctcg tctcaacagc gggcattccg
     2581 atattcggga ataccgcatc attaccaaag ccggggccat ccgatggctg cggacctata
     2641 gccgcccgat cattgaccct caaaccggtc tggtcactca gatttatggc gcctcgcagg
     2701 atgtaaccga ggtcaattcc gggcgactgt ttgaatcgct gatgcaccag cttgatcaaa
     2761 tggtcctgca ggaattaccg cttgaagata ttgtctcgta tgcctgtact cgattaacca
     2821 ccctgtttag ctattcggtg gcctgggtcg gattaaaaga gcaaactggc tcaatcagtg
     2881 tcaaaggata ttccgggctg tgggcccgcg attgtctctc acaactgccg ctggtctggg
     2941 aaggaacgcc tgaaacgctg ggactggctg gactggccat tacctcactc acccctcaga
     3001 ttgcccgccc gactgattta cacctgtggc actggcgaaa agctggtcgc tatggcgtcc
     3061 ggtcagccat tgccttgccg ttgattgcgc gtgggcagac catcggggtg attaacattt
     3121 atgcaaatca gagcgatgcc tttcggcagc cgtatctgtc ctggctcgaa gcactcgccg
     3181 cccggattgc gattgccctg ctgatttcga ttgatcgcct tcaactgcgc ctgcaaagtg
     3241 ccgcgctcga atcagccgcc aacgccatta tcattaccaa cgaactcggg aatattgaat
     3301 gggtaaatcc tgcgtttaca cgatttagcg ggtatacagc cgctgaagtt gccgggaaaa
     3361 acccgcgaat cctgcgctcg gaagagcatc cccgggcatt ttttgagaag ttctgggaaa
     3421 ccattcaatc cggccagatc tggcacggtg aactaatcaa ccgtcgaaaa gacggcgccc
     3481 tctatacggt tcgccagacg gtgattccgc tgctggatca acaaaacacg attactcatt
     3541 ttgtggcaat ccaggaagac atcacccagc aacgggaaga agaagcccgg atggtgaaga
     3601 tggcctatta cgacccgctg accaatttgc caaatcgaat cctgtttcag gatcggctcc
     3661 gacaggcact gctccaggga cgccggaatg actggcaggt tggcgtttta tttgttgatc
     3721 tggatcggtt taaacgcatc aacgacaccc tgggccatga tgccggcgat caactgatta
     3781 ccgaagtggc caatcgcctg agtgccagcg tccggcgcag cgattcggtt gcccggttgg
     3841 gaggcgatga atttacggtg attttaccgg acctccccga tacggatggc gcgctgattg
     3901 tggctcaaaa aattattacc gccctctcgg aaccaatcct cctcaaaggc catcaggttc
     3961 aaattacggc cagtgtcggc attgccttgt tccctgacca tgcgaccgat gctgaaggac
     4021 tggtgacgcg ggcggatatc gccatgtatg aagccaaaca gcggggcaaa aatacctttg
     4081 cactctatga accagggatg gacctgcaca gtgattcagc ctttatcctg gagcattcct
     4141 taccgaaagc cctgcaatcc aatcaattta tggttgatta tcagccaatc ttcaatgtca
     4201 ggtctggggt gccggtcgga gttgaagctg gtctctattg gaagcataaa gatcgtggtc
     4261 cgctggcgtt tgcccaattt gttgacctgg ctgagcagag cggaacatta aaacagatta
     4321 tgaactggta tctccaaacc gctattcacc agtttcagac ctgggccgat caggtaagcc
     4381 ctgaattcag gtttgttctg ccaatgcccg ttgcttcgtt gtgcgaaccc caaacaattg
     4441 actgcctcaa aaatctggtc caggaacttg gcctgtcatt ttcctggctt gaaattgcct
     4501 tgcaggaagc agaactggtc agcaccaccg gtaccgttca actcatcatt gaccagttac
     4561 gtgatgaagg tattcagttt tcactgcgtg gatttggtgt cgggaatttg ccgctggcga
     4621 ccctccaccg gttcaatttt catctggtca acctggatcc ggccttgatg tctcaactcg
     4681 gtgaaagcga gtcaagcgcc accctggtct ctgggttaac tgagtttctc catcacctgg
     4741 gaatcaaagt gggggctgaa ggagttgaag ccgagtgtca gataaaatta ttagcccaat
     4801 ttggatgtga tgaactgaaa ggaaagggaa ttgaatggtg gtttaacgct cccaactttg
     4861 acctgatctg tggaaaatca gccacggctg gagaatcgag ttttgtggct gtaaaatctt
     4921 ttgaatcatt ggataacccg ctacgaaaga tttcttgagg ttgaagtgtg gagttggaac
     4981 ctgctagaaa tagaaagctc tatattatgt tggacggaat tcacatgcac tctcacccgg
     5041 atgaacaaag ttcctaccct ggccccaaac ggtcatcctc ccagactggt taccaaaaat
     5101 ttcggttttt ggcagaccat ctggcacaac cagtgattga gtgggatgcg ttgctgatca
     5161 taaccaggat gaatgccgcc gctcaaacct cgtttgagca gagtggcacc gactgggttg
     5221 gacgctcact gaccgagctg atgtctgaac ctgttgaatt actcaaaaca cggttatcca
     5281 ccactggaag tggctggatg gaatatcgcc agggcgctgg atgcaaagga cactggtcct
     5341 gggttgccct gaaatcggca accagtgagt ccgagggggg aattgccatc atcaccagtt
     5401 cagccgttga tcctgaagcc gatcttaagc cggaacctgc ccggtttgtt ccaaccagag
     5461 aattatgtac tgtgttagat gccatccctg ccaacgtcgc actggttgac cgctttggca
     5521 tcatccaggt ggttaacgaa ccctggaaaa actatgcccg ggccaataat ttcaaagatg
     5581 acacgctcgg ccttgggtgg aattatctgg aggtatgtcg tcagagccag ggcagctcga
     5641 tggtggccga aacagcctat gtcctgctga aatcggtgct cgacgaacag tccgggcctg
     5701 aggagcttga atatccctgc catagtctat cggccaaaca gtggtttagc atgatcatta
     5761 ctccgatcaa actcgaaggc caaccatcag cgctggttat ccatactgac attactgagc
     5821 aaaaactggc tgagcaggcc ctgcgcaaaa gcgaagcccg ctaccgatca ctgattgcag
     5881 ccacggccca gtcagtctgg acgacccagg ccaatggaca ggtggtcgga gatcagccaa
     5941 gctggcgggc attgacaggc cagagcgtcg ctgacattca gggctggggc tggctggagg
     6001 ccattcaccc tgccgaccgt gagcaactcc agcaggtctg gatctatgcc ctggaaacca
     6061 aatcattatt gagcgttgaa catcgggttc ggattgcttc gggagaatac cggtcatttg
     6121 ccgttcgggc cgtgcccatg accgatgaag ccgggaaagt tgatgagtgg attggcacgc
     6181 acactgatat tacggatcaa aaaagagccc tcgacgaatt aaaaaaggcc gagacccagc
     6241 ttcgccattc ccaacgcatg gaggcggttg gccgactggc tggcggcgtt gcccacgatt
     6301 ttaacaacct gctcatggtt atctctggat atgccgatct ggtggccaac aaactgggca
     6361 aaacgcatgc gctgtaccga tttctggaag aaattcggca ggccagccgc tcggcctcag
     6421 gggggactcg ccagttgctc gcctttagcc gcaaacagg
//
LOCUS       DAOQDO010000044         6446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7142_length_6446_cov_3.597559, whole genome shotgun sequence.
ACCESSION   DAOQDO010000044 DAOQDO010000000
VERSION     DAOQDO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_6446_cov_3.597559"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKZ53_01335"
     CDS             complement(<1..1061)
                     /locus_tag="PKZ53_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNJ39105.1"
                     /translation="MIQNLLTRSLQRRADSLTITGRILYLTEDPTLIKQQLAGQDLDW
                     SPDNPALKLRDNISTDEITPGYVCYYYDETLGEFPYVGLKCGDQLPIGRNDIRQGGFA
                     VSVSGTRRGKGSSREQSPFAELTAGIQVVIAENIERIYNQNCQNLGILTSTNFALLDK
                     MKAGEEIPLAEFTTDADEITRQIIEYGGLFAFNLARLQGQVSIPEITTAPRPMTLAEK
                     IFAEHMVVDLAQNQLGVAAVKPGDAGFVRVDWRFSHEYVTPMAASFFEKYVGADEKVT
                     HPESVLLFRDHLTFLDQVLSPERRKLGLLDAAAGLAIKQQSFASQQGLTLHGELTDRK
                     GSEAICHSKMLQSYVLNSTA"
     gene            complement(1237..2652)
                     /locus_tag="PKZ53_01340"
     CDS             complement(1237..2652)
                     /locus_tag="PKZ53_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNJ39106.1"
                     /translation="MGFAINRAHFSRTFLKSHHIRWVGFGAWCALLLFSNWVPIPTVS
                     AEGPLAPVRMLVNQPVSEYKLRRQKLMDQTRDGIVVLIGNLDEGNGIDTKFRQNDNFL
                     YLTGVETPGAYLILVPDGYQGAKELLFIPPRNPNQERWTGPQIGPGEEAARLFNVDRV
                     LSSSDFYRVLYDITNAEFRNDWKKLYTIDFGNGAKFTREREFTDTLRQTLYNLQIVDV
                     TSKIGEMRRIKTAPEIALLQKAIEITGEAQRDAAQRLAPGRYEFELEALIIGAFLRQG
                     AERAGFPCIVGSGVYSTILHYNKNRKQIDDGDTVVIDIGAEYSQYTADITRTYPANGK
                     FTKRQRELYELVLAAQTEATKDFKPGQTTINDLNRVVRDFFHKSPLRGGNNLPMDYFF
                     IHGLTHYLGMNVHDVGDISAPLAPGTVITIEPGLYIPDEKIGIRIEDDYLVTEKGLEK
                     LSKSIPSQPDEIERLMAENPK"
     gene            complement(2716..5124)
                     /locus_tag="PKZ53_01345"
     CDS             complement(2716..5124)
                     /locus_tag="PKZ53_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ39107.1"
                     /translation="MNDKRASSAPQTTSSGDRLSSGPPSETFMTAPTTGANPNALKAA
                     LQREQAARNRLDALVKISSAVNSSRDLNGIFEVIAAEVRHLVPFDRASLAFLNEDRTR
                     VQVYALSSDDPASLQVGNNNLAEGTVTQWVVDRGEPVISNDLQAESRFVSHPNMLAQG
                     FRSSVSYPLIVREQKLGTLNFTSRQANCYSEADLDIIRLVADPIAIAVDNLRLLSLAE
                     MRLREAQAVAAREETINRIVSAMRRTLDTESILHTTAEQLGRHTKADRCLVYTLEDTD
                     RSGSVRASCFALDPAFSGAATWPTIREAITSNLIQGRHLSFPDTSADSAPAAINSLHR
                     IFEIGSVVYVPVISQDKLTAVIELNQNTAREWSESDVTLVKAVADHLAVSLYQSQLHH
                     EVIRHSAENAHLVNELRETNQKLAKVNKLKDEFLANLSHELRTPLTAITAWAELLDTL
                     YARDLGLRTPELSEGLNTILNSSTALTQIIEDLIDLSRIQNDKLDLDLIPTDIHAPIK
                     EALQMIRQQAKNKQIQLELRQDPHLPAMPVDSQRIRQVIWNLLSNSLKFTPPNGKIIV
                     TTQLRDNQVEILVQDSGIGIEPSFLPYVFDRFRQEEGTKKRRFQGLGIGLTITKALIE
                     AHHGQISVSSAGRNQGTTFTLTLPLPVKTLHTLPAATESTKGTVDTPSSQREILVVED
                     DPLVLRLVGRMVEHLGYTPHLAESGHLALQLLEQWTPALILTDINMPEMDGFEFLTML
                     RKSPHTAHTPVVALTAFVTNTDRAHMEKAMFDGILAKPVRQYQLAEMIQKQLTVECGG
                     SPPL"
     gene            5638..6030
                     /gene="ndhC"
                     /locus_tag="PKZ53_01350"
     CDS             5638..6030
                     /gene="ndhC"
                     /locus_tag="PKZ53_01350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNJ39108.1"
                     /translation="MQPDSLLAYFPILMVFFLAAGTALVMLNTSRLIGPRLNTREKLM
                     PYECGKDPVGSAHERFSVKFYLICLLFILFDIEAIFLIPWAVVFKDLAAISQGFKIFV
                     FSEMMVFITILFIGYIYIWKKGVFRWNK"
     gene            6129..>6446
                     /gene="nuoB"
                     /locus_tag="PKZ53_01355"
     CDS             6129..>6446
                     /gene="nuoB"
                     /locus_tag="PKZ53_01355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNJ39109.1"
                     /translation="MGLETKLADMLPEVGLTTLDFLVNWSRKSSLWPATFGLACCAIE
                     MMNITSARNDLARFGAEVFRASPRQADVMIVAGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGAC"
BASE COUNT         1423 a         1578 c         1775 g         1670 t
ORIGIN      
        1 gcggtgctgt tcagcacata ggattgcagc atcttgctgt gacagatggc ttctgaccct
       61 ttgcgatcag tgagttcgcc atggagtgtg agtccctgct ggctggcaaa agattgctgt
      121 ttgatggcga gtccggcggc ggcgtcaagc aacccaagct tgcggcgttc tggcgacaaa
      181 acctgatcca gaaaggtcaa atggtcgcgg aacagcagca ctgattccgg atgggtgact
      241 ttttcatcgg cgccaacata cttttcaaaa aagctggcgg ccatcggtgt tacatattca
      301 tggctaaatc gccagtcaac ccgcacaaac ccggcatctc ctggtttgac ggcagcgacg
      361 ccaagctggt tttgagccag atctaccacc atatgttcgg caaaaatctt ctctgccagc
      421 gtcatcgggc gcggcgcggt ggtgatttcc ggaatgctga cctggccctg gagtcgggcc
      481 agattgaagg caaagagccc gccgtattcg ataatctggc gcgtgatttc atcggcatct
      541 gtggtgaatt cagccagtgg gatttcttca ccagctttca ttttgtcgag gagggcgaaa
      601 ttggttgacg tcaggatgcc caggttctgg cagttctggt tgtaaatgcg ctcaatattt
      661 tcagcaatca caacctggat tccggcagtg agttcggcaa acggcgactg ctcgcggctg
      721 gagccttttc cacgacgggt gcctgaaacc gaaacggcaa aaccaccctg tcggatgtcg
      781 tttcgaccaa tcggcaactg gtcgccgcat ttcaacccga cgtagggaaa ttcacccaat
      841 gtctcgtcat agtaataaca cacataccca ggggtaattt cgtcagtgct gatgttgtcg
      901 cgtaatttca gggctgggtt atctggtgac cagtcgagat cctgaccagc cagttgctgt
      961 ttgatcaggg ttgggtcttc ggtgagatac aaaatccggc cagtaatcgt gagcgaatcc
     1021 gctcgacgtt gaagcgaacg agtaagaagg ttttgaatca tagcgtgtag gtagaggggg
     1081 tggagatagg gttcagggtt cagggttcag ggtttcgggg tttgacggga aaggttccgc
     1141 gcttcggggt tttcgaaatt tatgtccttt tcgtcttttt cgtccttgtg gtcttttttg
     1201 tcttttccct gaaccctgaa ccctgaaccc taaaccctac tttgggtttt ccgccatcag
     1261 gcgttcaatt tcatctggtt gggaaggaat acttttggag agtttttcca atcctttttc
     1321 agtaaccaga taatcatctt cgattcggat cccgattttt tcgtccggga tatagagtcc
     1381 aggctcgatg gtaatcacgg ttcctggtgc caatggtgct gagatatctc cgacgtcgtg
     1441 gacattcatg ccgagatagt gggtcaggcc atggataaaa aagtaatcca tcggcaggtt
     1501 gttgccaccg cgcaacgggc tcttgtgaaa gaaatcacgc accacacgat ttaaatcatt
     1561 gatggttgtc tggccaggct taaagtcttt ggtggcttct gtctgggcag ccagcacaag
     1621 ttcatagagt tcacgctggc gtttggtaaa tttcccgttg gctggatagg tacgggtgat
     1681 gtcggcggtg tactggctgt attcagcgcc aatatcaatc accacagtgt ctccgtcgtc
     1741 aatttgtttc cgatttttgt tgtaatgcag gatggtggaa tacacaccag agccgacaat
     1801 gcagggaaag cccgcccgct cggccccctg gcgcaaaaag gcgccaatga tcagggcttc
     1861 cagctcaaat tcatatcgcc ctggtgccaa tcgttgcgcg gcatcccgtt gcgcctcacc
     1921 cgtaatttca attgctttct ggagcagggc aatttcaggt gccgttttga tacggcgcat
     1981 ttcgccaatt ttcgaggtca catccacaat ctggaggtta tagagcgtct ggcgcaaggt
     2041 atcagtgaac tcgcgttcac gggtaaattt tgccccgttt ccaaagtcaa tcgtgtagag
     2101 ctttttccag tcgttgcgaa attccgcatt ggtgatgtca tagagcaccc ggtaaaaatc
     2161 gcttgatgaa agaacccgat ccacattgaa caatctggcg gcctcttcgc cgggcccaat
     2221 ttgggggcct gtccagcgct cctggtttgg gttgcgtggt ggaataaaca acaattcttt
     2281 ggcgccctga tacccatcag gcaccagaat caaataggcg ccaggtgttt ccacccccgt
     2341 cagatacaaa aagttatcat tttggcgaaa cttggtgtca attccgtttc cttcgtccag
     2401 atttccaatc aagaccacaa tcccgtcacg ggtctggtcc atcagttttt gacgacgaag
     2461 tttgtattcg ctgactggtt gattgaccag cattcgaacc ggtgctaacg gtccttcggc
     2521 tgaaactgtt ggaatcggca cccagttgga aaacagaagc agggcgcacc acgcaccaaa
     2581 tccgacccag cgaatgtggt gtgacttcag gaaggtgcgg ctgaaatgtg cacgattgat
     2641 ggcaaaaccc atgaaaacac ctcttggaaa acatcaaggc ggcgtcaagc cgccgcactc
     2701 cacagttttg aaacattaca gcggcggcga gccaccgcac tccacggtca gttgtttttg
     2761 aatcatttcc gccagttgat attgccgcac tggcttggcc agaatgccgt caaacatggc
     2821 tttttccatg tgggcccggt cggtgttggt aacaaatgcg gttaaggcaa ccactggcgt
     2881 atgggccgtg tgcggtgact tgcgcagcat ggtcagaaat tcaaacccat ccatttcagg
     2941 catgttgatg tcggtcagga tgagcgccgg cgtccattgt tcaagcagtt gcagggcgag
     3001 atgaccgctt tcggccagat gaggtgtata gcccaggtgt tcgaccatgc gtccaaccag
     3061 tcggaggacc agcgggtcat cctcaaccac caggatttcc ctctgcgacg atggggtatc
     3121 caccgtcccc tttgtacttt cggtcgcggc gggcagtgta tgcaacgttt tgactggcag
     3181 tggcagcgtc agggtaaagg tggtgccttg gttgcggcca gcacttgaga cgctgatttg
     3241 tccgtgatgg gcttcgatca acgccttggt aatggtcaac ccaatgccca gaccctggaa
     3301 acggcgtttt ttggtgcctt cttcctggcg gaaacgatca aacacatagg gcagaaatga
     3361 cggttcgatt ccaatcccgc tgtcctgcac caggatttct acctgattgt ctcggagttg
     3421 cgttgtgacg atgatcttac cgttgggagg cgtaaacttc aggctgttgc tcaacaaatt
     3481 ccaaatcacc tgccggatgc gttgtgagtc aaccggcatg gctggaagat ggggatcctg
     3541 tcgaagttca agctggatct gcttgttttt tgcttgctgg cggatcatct gcagcgcttc
     3601 tttaattggc gcgtggatgt cggttgggat caggtcaagg tcaagtttgt cattttgaat
     3661 ccgcgagagg tcaatcaaat cctcaatgat ctgagtcaat gcggtcgaag aattgagaat
     3721 ggtgttgagc ccttcgctga gttccggagt gcgcagtcca aggtctctgg catagagggt
     3781 atccagcagt tcggcccagg ccgtaatggc ggtcaacgga gtgcggagtt cgtgcgagag
     3841 attggccagg aattcatctt tcagtttatt gactttggcc agcttctgat tggtttcgcg
     3901 aagttcattg accaggtggg cattttcggc gctgtgccgg atcacttcgt ggtgaagctg
     3961 ggattgatag agtgatactg ccagatgatc agccacagct ttgaccaggg tgacatcgct
     4021 ttcgctccat tcgcgagcgg tgttttggtt gagttcgatc accgccgtca atttgtcttg
     4081 cgaaatcacc ggtacataaa ccacggaccc aatttcaaaa atacgatgca ggctgttaat
     4141 ggctgccggc gctgagtcag ccgaggtatc agggaaggac agatgccggc cctggatcaa
     4201 attagacgtg attgcttcac gaatcgtggg ccaggtggcg gcacctgaaa aggctggatc
     4261 cagagcaaaa caactggccc gaaccgaacc ggagcgatca gtgtcttcca gtgtgtagac
     4321 caggcagcgg tcggcctttg tatgtcgccc gagttgttcg gcggtggtgt gcaaaattga
     4381 ttcagtatcc agcgtgcgcc gcatcgcact gacaatgcgg ttgatcgttt cttcgcgggc
     4441 ggcaacggcc tgggcttcgc gcaggcgcat ctcggccagc gagagcagac gcaggttgtc
     4501 aaccgcaatc gcaattggat ctgccaccag tcgaataatg tcaagatcgg cttcgctata
     4561 gcagtttgcc tgacggctgg tgaaattgag cgtgccaagt ttttgttcgc gaacaatgag
     4621 tggatatgac accgatgacc gaaagccctg ggccagcatg ttgggatgcg aaacaaacct
     4681 tgattcagcc tgcaagtcat tgctaatgac tggttccccc cgatcaacca cccattgggt
     4741 gacagtgcct tcagccaggt tgttgtttcc gacctggagt gaagctgggt cgtccgacga
     4801 cagggcatag acctggactc gggttcgatc ttcgtttaaa aaagccaggc tggcccggtc
     4861 aaatggcacc agatgccgga cttcagccgc aatgacttca aagatgccat ttaaatcgcg
     4921 gcttgaattg acggcactgc tgattttaac cagggcatcc agtcgattcc gggcagcctg
     4981 ctcccgttgg agggcggcct tgagtgcatt tggattggcc ccagttgtgg gtgctgtcat
     5041 aaaggtctct gacggcggac cggatgacag tctgtccccc gaactggtgg tttgaggggc
     5101 ggaggaagct cgtttgtcgt tcatcgtgat gtgtcatttc cctcgacaga aaagtacggt
     5161 tgggccgcag gtcggtcaaa ccgttcccgg cacgcaacat aaagagttgt gatggatgac
     5221 gatgtcgtga cagcaacttg actgatggaa gccatacata ataaggataa gaggtggttt
     5281 ggagcaaggg gcgcaaggat cttcccatca gggaaggcag cctgtccttg acactccaaa
     5341 gtcggctggc ctagtatcac ggagcaaatt cttgcccccg cgtttttcct aacatcttga
     5401 cgcagcaacg ctttatgccg atcagccggg aataccatcc cgtcctggcg catgctttgc
     5461 cgcacaaacg ctgtggtact cttcagtcct ctactttctg tgtctggtgt ctggtgcttc
     5521 gtctgaaacc cctggcctct cacttgtgta ccaaaaacca gagactccga aaccaagatt
     5581 gcgcctcatc aattgatctc agcgttgatt ggtgcgcact gtacgaagga ttccgtcatg
     5641 caacctgaca gcttgcttgc ctattttcca attttgatgg tgttttttct ggctgccgga
     5701 acggcgctgg tgatgctcaa tacatcccgg ttgatcggcc cccgcctcaa tacccgtgaa
     5761 aagttgatgc catacgaatg cgggaaagat ccagttggat cggcccacga acgcttttca
     5821 gtgaagtttt acctgatctg cctgctcttt atcctctttg atattgaagc cattttcttg
     5881 attccctggg ccgtggtgtt taaagacctg gctgcgattt cacagggatt caagattttt
     5941 gtattttctg aaatgatggt gtttatcacc attctgttca ttgggtatat ctacatctgg
     6001 aaaaaaggcg tctttcgctg gaacaaatag gtgaatgaag aaaaaatctt tcggttgaag
     6061 attcaaactt catctgattg attatcaaag agttaacgaa ttcagaattc agaagtgagg
     6121 gttgatctat gggtttagag acaaagcttg cagacatgtt gccggaagtt ggtctgacga
     6181 cattggattt tctggtgaac tggtcgcgca aatcatcatt gtggccggcg acttttggat
     6241 tggcctgctg tgcaattgaa atgatgaata tcacctctgc tcgcaatgat ttagcccgtt
     6301 ttggcgctga agtgtttcga gccagtccgc gccaggctga tgtgatgatt gttgcgggcc
     6361 gtgtctcgcg caaaatggcc ccggtcctgc ggcgaatcta tgaccagatg ccagagccaa
     6421 agtgggtgat ctcaatgggt gcctgc
//
LOCUS       DAOQDO010000045         6400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7224_length_6400_cov_2.921828, whole genome shotgun sequence.
ACCESSION   DAOQDO010000045 DAOQDO010000000
VERSION     DAOQDO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7224_length_6400_cov_2.921828"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKZ53_01360"
     CDS             complement(<1..1064)
                     /locus_tag="PKZ53_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39110.1"
                     /translation="MTKPKSKNSNEDVLRKPAAKKLGLKINPIGMPHDFLLRNQEKTE
                     PEPLAAAPSTMPEATLTQPPPAQNPRHTEVSNPSYTGIQTQSYTEVQTPGYTRPYLTS
                     QTTENKEENQAQTESYTEVQTPSYTNSELYSSLYSSLYTGSNPRIADHQTQSYTEVQN
                     PLKGKKTKLVAIRLNIDLIELIKSECQQSGETFTVFIESAAIQRLKQAAIQRSNPVYN
                     SPPAPPHDDILMIDDNIIRAHARFAIQPWSPRDDRVGRRFNGTDPRLIDIAMILTVER
                     KLRGNTSRIPIKSFSYFVPELEGLIEDYRENRLPLSLDDYHRYALDAWERRIKPLRDE
                     KWQLNETEPAEKKPPSKKQN"
     gene            complement(1072..1887)
                     /locus_tag="PKZ53_01365"
     CDS             complement(1072..1887)
                     /locus_tag="PKZ53_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ39111.1"
                     /translation="MHILAVANQKGGVGKTTITRELSACFALRGYQVLAIDCDPQGNL
                     TQSWVNTDVFEGTLAHVLIEPEIQQGQRREPLSIADTIVESPIKNLDLIPCDIRLARF
                     EQQPEYLMHRLRKQLYEHASTYDLVLIDCPPQLGKLLSAALYAAHYVVIPCAADAMGL
                     QGLADLAHTIDQVRSNVNAELQTLGVVVNLYKPTRNLSAESRAAIEDVLPLVKHVFDT
                     NIHDYAKIAEAPSQHLPVVIYSEDHRAADLFWALTDEILDRLNMTRQKISVVR"
     gene            complement(2141..3808)
                     /locus_tag="PKZ53_01370"
     CDS             complement(2141..3808)
                     /locus_tag="PKZ53_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ39112.1"
                     /translation="MQNFIRGQKARLSELVSGLSFEVVLQVGFSSQTPVDYCCFVVDA
                     SQRILDDRYLIYSRQMQSPCGSVRLLDGPGSPAARFLVNLASLPKQSQKLVFTATING
                     APVMSQLAYGALQLIGSNGPAIQFPLSGADFSGEKSIIIGEVYFKDDWRIAAVGQGFS
                     EGLNTLLRKFGGNQPGGAAGAGPAQSPEIPTLVQPVPASRPPAAYQTMPITSARPDTS
                     PNLPLQATTPFGPTNQPPGMPQPAAAPRAPQPPMPPTVAAPMAPPPYHPGVQTPAGSG
                     IPTVAAGIPPASNTPYGNVPGAPPPQNQPWGASPNNPSPYTPPPPPTHAPRQPGSKLG
                     KILAIGALLVVILFGGLIGIGFLVTKLKPKPTAEELARERQEKELKAFAKEVTTLSGL
                     DSGSGRGYTTGKIYIVDKGLGIGDSLNYRVKPEWQAKSKDEIGTVVLVKKDSKVIGQY
                     DLEGNGGKVDAKVYVWTVSVIDLKNKLITIEDELIGSDPPLSIKRKKGDRSPEFGGDP
                     KLIEFIEKLDRKAVSGSDSSKSSGTDTEAPPKDPTGSPTAGEGWKQP"
     gene            complement(3921..4829)
                     /locus_tag="PKZ53_01375"
     CDS             complement(3921..4829)
                     /locus_tag="PKZ53_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ39113.1"
                     /translation="MAKKLRWSVSAERLAQLSQLSQETPVGSPAVDEPSRPRPENPQV
                     RWVDLEQIQPNPFQPRREMSADGLDDLSSSIQMHGLLQPLVATPNTDGSYTLIAGHRR
                     WAASRQAGLKQAPVLVRSVNEPELQVLALIENIQREDLHVVDKALALADLSKKLPTAK
                     DAAKVVGLKRTTLANWLRVAELNEAVLTLCRQNPEATLNGMLQLSRLASNQQIPAAKR
                     WLSRVKNHPSTGQETRPKPQDTAGSTTQRLTVYLPEREFSCTVEVRSHAHRAALSEED
                     VVRCLQLAIDQLREKLVTSSNQPVSV"
     gene            complement(4822..5667)
                     /locus_tag="PKZ53_01380"
     CDS             complement(4822..5667)
                     /locus_tag="PKZ53_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ39114.1"
                     /translation="MTTGQNNCVKTIAVFNQAGGVGKTTLTRDLGFELAGRGARVLLV
                     DADPQGTLGVFFGETPAERPVTDLFWSSICMRGGPKGDPIRIHSEFGVHLGLANHTLI
                     DDEWVLAQQHDPARLLGVFDRLKAEYDLILIDCPPKIAELTHQVLLAADALLIPVQPE
                     GKAVVAFAEVHREILQVQQRRTNMRLPTIAVLGVVPTIFNPRLVLHRHHLEELRDRIC
                     ASFGYPLFPAIRDYVSVAEAGTQGLPLKAYDARCAANQDVSAVADMLLDQIKVPNPLS
                     EVVNG"
     gene            complement(5816..>6400)
                     /locus_tag="PKZ53_01385"
     CDS             complement(5816..>6400)
                     /locus_tag="PKZ53_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39115.1"
                     /translation="VILVASQRFKSPTDPGNPQLARQQPPTRVEVPTAPEPGVPEPAP
                     QTGANPPATPSVPKPKGPKPSKPATPTQPAVEKPVEPVFAVTLLPLSLRSNEEEPAPM
                     IPQQVKVVTLKLLLDPVASFPHYNVEILTPDGNAIYRANHLKPGGKAPAQTLTLRFPV
                     SNLEDGDYRIQLNGLVKGTEEPASVYIFSLRKQK"
BASE COUNT         1428 a         1576 c         1764 g         1632 t
ORIGIN      
        1 ttattttgtt ttttggatgg tggttttttc tcggcaggtt cggtttcgtt gagttgccac
       61 ttttcgtccc gcaggggttt gatgcgccgt tcccacgcat ccagcgcata acggtggtaa
      121 tcgtcaagcg agagcgggag gcggttttca cggtaatcct cgatgagtcc ctcaagctct
      181 ggcacaaagt aggaaaaact cttgatcggg atacgagacg tgtttcccct gagcttgcgt
      241 tcaaccgtca agatcatcgc gatgtcgatc agcctcgggt ctgtcccgtt aaaccgcctc
      301 cccacacgat catcacgcgg cgaccagggt tgtatagcaa atctcgcatg cgcgcggatg
      361 atattatcat caatcatcaa aatatcatca tgggggggtg caggggggga gttatacacg
      421 gggtttgaac gctgtatagc agcctgtttc aacctctgta tagcagccga ctcaataaag
      481 actgtgaatg tttcaccgga ttgctggcat tctgatttta taagttcaat taagtcaata
      541 tttaaccgga ttgcaacgag tttagtcttc ttgcctttga gagggttttg aacctctgta
      601 tagctttggg tttgatggtc tgctatacgg gggtttgaac ctgtgtatag cgagctatac
      661 agcgagctat acagttctga atttgtatag ctgggggttt gaacctctgt atagctctcg
      721 gtttgagcct ggttttcctc tttgttttca gttgtttgag aggtgagata aggtcttgta
      781 tagccagggg tttgaacctc tgtatagctt tgagtttgaa tgcccgtata actggggttt
      841 gaaacctctg tatgacgggg attttgagcc ggcggaggct gtgtaagcgt ggcttctggc
      901 atcgtggatg gcgctgcggc gagcggctct ggctcagttt tttcctgatt tcgcaacaga
      961 aaatcatggg gcatcccaat cggattaatc ttgagcccca gctttttggc ggctggttta
     1021 cgcaatacat cttcgtttga attttttgat tttggtttgg tcatggcgct cttacctcac
     1081 caccgaaatt ttctgacgag tcatgttcag tcggtccagg atttcatcgg ttaatgccca
     1141 aaacaaatct gctgcccgat ggtcttcgct atagatgacg actggtaaat gctgagatgg
     1201 ggcttcggca atcttggcgt agtcgtgaat gttggtatca aaaacgtgtt tgaccagggg
     1261 taagacgtct tcaatcgccg ctctggactc tgccgacagg ttccgagtcg gtttatacag
     1321 gttgaccacc acgccgagcg tctgcaattc ggcgtttaca ttgctccgta cctgatcaat
     1381 ggtatgggcc agatctgcca ggccctgtaa tcccatggca tctgcggcac aggggatcac
     1441 tacataatga gccgcataca aggctgccga cagcagtttg ccaagctgtg gcggacaatc
     1501 aatcagcacc agatcatagg tggaggcgtg ctcatagagt tgttttcgga gccggtgcat
     1561 taaatattcc ggctgttgct caaagcgggc cagtcgaata tcacagggga tgagatccag
     1621 atttttaata ggtgattcaa cgatggtatc ggcaatggac agtggttcgc gccgttgccc
     1681 ctgttgaatt tcaggttcaa tcaggacgtg agccagcgtg ccttcaaaca catcggtatt
     1741 gacccaactt tgggtgagat ttccttgtgg atcgcagtca atggccagaa cctgatagcc
     1801 gcgtaaggca aaacaggctg ataactcgcg tgtaatcgtt gttttgccaa ctccgccttt
     1861 ctggttggca acggcaagaa tatgcatgac aaaacccctt tcaaaatcag tgagcatcga
     1921 aaccaaaagc accgtggcgc ctgctcatca taagagtgaa cttctgattt ggcaaaaaag
     1981 accattcact ggaaagaaaa atcggctggt tgaaaataaa ttggcctgaa ttggatttca
     2041 ggccaacatc ggggcgcaca ttaaccgtaa cctcgaaata ttcacgaagg aacgcgagcg
     2101 gcctcattaa actttggggt ttttccttcg tgaatttttt ttagggctgt ttccaccctt
     2161 ctccggcggt tggtgagcca gttggatctt ttggtggtgc ctcagtatcg gtaccagatg
     2221 actttgacga atcagaacct gaaacggctt ttcggtccag tttttcaata aactcaatca
     2281 gttttggatc gccgccaaat tcaggcgacc gatctccttt tttccgtttg atgctgagcg
     2341 gtgggtctga cccaatcagt tcgtcttcaa tcgtgatgag cttatttttc aaatcaatga
     2401 ctgagaccgt ccacacatac actttggcgt caactttccc gccattgcct tccaaatcat
     2461 actggccaat cacttttgaa tctttcttga ccagaaccac ggttccgatt tcatctttgc
     2521 ttttggcctg ccactcaggt ttcacccgat aattgagcga atcgccaatc ccaagtcctt
     2581 tatcgacaat atagattttt ccagtggtat agccgcgacc tgagcccgaa tccagcccgg
     2641 aaagcgtggt cacttctttg gcaaacgcct tcaattcctt ttcctggcgt tcgcgggcaa
     2701 gttcttcggc ggttggcttg ggtttgagtt tcgttaccag aaaacctatt ccgatcaacc
     2761 ccccaaacaa aatcaccacc agcaaggcac caattgcgag gattttgcca agtttcgacc
     2821 ctggttgacg aggtgcatga gtcggcggcg gaggtggggt atagggggaa ggattatttg
     2881 gtgaggcacc ccagggttga ttttgtggag gcggagcgcc gggaacattg ccataggggg
     2941 tgttcgatgc aggtggaatc cctgctgcca ccgttggaat cccagatccg gctggtgttt
     3001 gaactccggg gtgatagggc ggcggcgcca ttggggcggc cactgtcggc ggcattggtg
     3061 gttgaggtgc ccgtggggcc gctgctggtt gaggcattcc tggcggctga ttggttgggc
     3121 caaatggtgt ggttgcctgg agtggcaaat ttggcgatgt gtcgggtcgg gcgctggtaa
     3181 tcggcatggt ttgataggcg gctggcggac gtgatgccgg caccggctga accagcgttg
     3241 gaatttccgg agactgtgcc gggcctgccc cagcagcgcc cccaggttga ttccctccaa
     3301 acttccgcag cagcgtgttt aagccttcgc taaatccctg gccgacagcg gcaatccgcc
     3361 agtcatcctt gaaatacact tcaccaatga tgattgattt ctcgcctgag aaatcagccc
     3421 cactcaacgg aaactggatg gcagggccat tgctcccaat aagctgaagc gcgccatagg
     3481 caagctggct cataaccggg gccccattga tggtggccgt aaataccagt ttttgtgatt
     3541 gtttcggtaa actcgcaagg ttgaccagaa accgggctgc cgggcttcct ggaccatcga
     3601 gcaatcgaac tgacccgcag ggagactgca tctggcggga ataaatcaaa tatcggtcat
     3661 caagaatccg ttgggaagca tcaacgacga aacaacaata atcaacgggt gtttgactgg
     3721 aaaaaccgac ctgtaacaca acttcaaatg agagaccgct gacgagttca gataatcgag
     3781 ctttttgtcc acgaataaaa ttttgcatag tgcatccttc ccatccaaca aattgattca
     3841 ataatctcag cccggcggaa aaggttcctc cgaaacaaca gtgctggcaa agatcaaagg
     3901 gtaaaaaaga gtctgaaaaa tcaaaccgaa actggttgat tggaactggt gaccagtttt
     3961 tcccggagtt ggtcaatggc gagttgcaaa catcgcacga cgtcttcctc tgaaagggcg
     4021 gctcgatggg cgtgagatcg aacctcaacc gtgcaggaaa attcccgttc cgggaggtac
     4081 acagtcaggc gctgggtggt gctaccagcc gtgtcctgcg gtttgggtct ggtttcctga
     4141 ccggtggagg gatggttttt gactctggaa agccaccgtt tggccgccgg gatctgctga
     4201 ttggaagcca gtcgggaaag ctgaagcatt ccattgaggg tggcttccgg gttttgacga
     4261 cacagcgtga gcaccgcttc attgagttcg gccacacgaa gccagttggc aagcgtggtt
     4321 cgtttcaaac caaccacctt cgccgcatct ttggcggttg gcagcttttt agaaagatca
     4381 gccagtgcca gcgccttatc aaccacgtgc aggtcttcgc gttgaatatt ttcaatcagc
     4441 gccagcacct gaagctcagg ttcattcact gaccgaacca gaaccggcgc ctgtttcagc
     4501 ccggcctgac gtgacgctgc ccaccggcga tgaccggcaa tcaaggtata gctcccatct
     4561 gtatttggtg ttgccaccag cggctgcaac aggccgtgca tctgaatact ggaactgagg
     4621 tcatccaacc catctgccga catttcacgc cggggttgga atggattcgg ctggatctgt
     4681 tcgaggtcaa cccaccggac ttgtggattc tcaggccggg gccgcgatgg ctcatcaacg
     4741 gcaggtgaac cgaccggtgt ttcctggctc aattgtgaga gttgcgccag gcgttcagcg
     4801 gaaacgctcc agcggagttt tttagccatt gaccacctcg ctcaacgggt tggggacttt
     4861 gatttggtca agcagcatat cagccacggc gctcacatcc tgattggcgg cacatcgggc
     4921 atcataggct ttgagcggca ggccctgggt tccagcctca gcgacgctga cataatcacg
     4981 aatcgcaggg aacaacggat agccaaacga ggcacaaatc cggtcacgga gttcctctaa
     5041 atgatggcga tgcaacacca gtcgggggtt aaagatggtc ggcaccacac ccaaaaccgc
     5101 aattgtcgga agccgcatat tggttcgtcg ttgctggact tgcaggattt cgcggtggac
     5161 ttcggcaaag gcaaccactg cttttccttc aggttggacc gggatcagca gcgcatctgc
     5221 cgcgagcagt acctgatggg tgagttcggc aattttcgga gggcaatcaa tcaagatcag
     5281 gtcatattca gctttcaacc gatcaaagac gccgagcagg cgggccgggt cgtgttgttg
     5341 agccagaacc cattcatcat caatcaaagt atgattggcc agcccaagat gaaccccaaa
     5401 ctcagagtga atgcggattg gatcgccttt ggggccacca cgcatacaga ttgatgacca
     5461 gaaaagatca gtgacgggtc gttctgccgg ggtttcccca aaaaacacac caagcgtgcc
     5521 ctgcggatcc gcatccacca gcagcacgcg tgccccacgc ccggcaagtt caaagcccag
     5581 atcccgagtc agggttgttt tgccgacacc tccggcttga ttaaacaccg ctatcgtttt
     5641 gacacaattg ttttgtcccg ttgtcattgt aaaaccgaaa agtaatttga gtggattgat
     5701 ttctcagtcg gaccgatggc ttgaaactaa accgaatcgc cccagaactc aagtccctgg
     5761 agtgcgacga cttgacgtcg ctttggtttt acaactcacg tttggctggt gggtgctact
     5821 tttgtttgcg aagcgagaag atataaacgc tggcgggttc ttccgtgcct ttgaccaggc
     5881 cgttgagctg gatccggtaa tcaccatctt ctaagttact caccggaaat ctgagcgtca
     5941 gggtttgagc cggggctttc ccaccaggct tcaggtgatt tgcccggtaa atggcattgc
     6001 catctggggt caggatttcc acgttgtagt gtgggaagct ggccaccggg tcaagtaaaa
     6061 gcttgagcgt tacgactttg acctgttgag gaatcattgg ggctggttct tcttcgttgc
     6121 tgcgcaggga aagtggcaac agcgtcaccg caaagactgg ctcaaccggc ttttcaacgg
     6181 ctggttgcgt cggcgtggct ggttttgaag gtttgggtcc cttgggtttg ggaactgatg
     6241 gtgttgcagg ggggttagcc cccgtttgtg gggctggttc tggaacccca ggttcgggtg
     6301 ccgttggtac ctcaaccctg gtgggtggtt gttgccgggc aagctgtggg tttcctggat
     6361 cagtggggga cttaaaccgt tggctggcga ccaggatcac
//
LOCUS       DAOQDO010000046         6363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7288_length_6363_cov_3.347971, whole genome shotgun sequence.
ACCESSION   DAOQDO010000046 DAOQDO010000000
VERSION     DAOQDO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7288_length_6363_cov_3.347971"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..3724)
                     /locus_tag="PKZ53_01390"
     CDS             complement(113..3724)
                     /locus_tag="PKZ53_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNJ39116.1"
                     /translation="MIRDYLKNVIGDGSSGEVCLQWLFCPLGSHHLGLAQLEKESRQQ
                     AFNTLVQKLNLPAEKSVLLANGPAGKGRFRDADWISLIKKGRRWHVLVLEFSLYAANS
                     AHHLPDPGDPAAVLNHFRSVVGLLRRRGAYSQLNLDLTENDIAVSSEMSNYLDGIRRA
                     DKPAKKLFQGCAYATDFLDRLAEVGFEMKKGLVQVIAVTDRGTQSIAAQFEHRNRLRP
                     FAEIIWNQTAGKGESWEEVQSRQRKVAFRTLVSSFPIEARKEIRKLREVALSRLESAT
                     LLTAEIQEPITQTHNPAEPVTLEQIQEWLTTPEIQNWIPDQQKQIQKSLKLYQNPTLR
                     DAHALFVQALLDRDYPDARIPVIALTGHPGIGKTTAIRNSLLMRPHGFLFLYFSPRIQ
                     INRDILGNFENQSEPPIVTLTTNAATIDEFIQIREAERRHHRELQPISGAVVYGGCGV
                     CLPEEFKNVRLVRDKVEREIEAGPAVNRERRYREQSVDTIVPRSSDAKGVLACLSAGI
                     ECARKTGIDRIVAAVSTQALKAVVNGKKTIDALGAIVPQKNKSPWLGGLDQFAARIPE
                     IVVMLDELTGSSEGVMLLHDIVSRLDQWFVTPYDEANRKSPFRIRVIVADASLTDERV
                     AERFLAETRPEPPKIIVSPAGSSNPISLGSFHFCGQAVPLLNASSFPARSLTLNYHLT
                     LVPAQIGDHGRVEAKPTELTPILNDRLVEAVITALSEGARQIIVFVQDIRRLDDLAEE
                     LKSACGFKGPDILKINSRIRNSERARIIEEKHHLSARVILITSSASRGISFPLTTVIL
                     AEVPRFAPEQSLMEIVQLIYRGRGTAHCKDGHIINGDYVDRRIEFFLTETLGPSNSEE
                     RINDLLAMCVLLRAAVMTRICGVGYIGDQAVSVVPVGVKLVEISDQTDTEEIINFLGE
                     LEKELHATSNSALRSVCQLTHNAMCMMFRTHGNHRIINISKQSQSTRLDAEIFRRDWI
                     ERSLVPSIRNGLDGIVTIPEFPVEAMYCSGLLVEERVGEMSDELIILNREESRATTRQ
                     LKGLLQALRTGLLTSKDGFTTKLDIRSRQLMSGAARMVDLLDSLFYSKASGDITQDRQ
                     RLEHRHREGVWLAQPALCFSKVSRWSEHFSISSREIHREWYDLISELTRIQFGKAQRI
                     APVVAAYDDIPFGVFRLNGVEMFREQIFSPSAFNATHEFQLLNIFLLRQQEQTENPG"
     gene            complement(4000..>6363)
                     /locus_tag="PKZ53_01395"
     CDS             complement(4000..>6363)
                     /locus_tag="PKZ53_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39117.1"
                     /translation="RHRQRTDADPTRLHALVQTIREKGMGRVEREIGLMLLKALCEAA
                     TSNKKIPELTQYLAGVSALGKFGEAEGLEDQDLELSMGVFGVADRFSFRQEMMLTSAF
                     DSLPIRIISSGFITDEVFGPRETQSIANSYHVILNGPIALSEERRFESVLEYRLDQIE
                     RALSLGNEEDEGSRRFLARKAMCNLATLWLCLPDGRSLSLQDRFMNLKQLLGLGRDGV
                     REGLSELRSRTQKVNIIATSLHTLLKERKDVLGTLVRQPFDLYLCLLPSILDRVGIKQ
                     AKPFLSFPPDREEVGYLSHLTVTQEAPNGMFASFPITVHFSERWVSRANKESINRYIL
                     QRELPEKVVYLVIYPGREYLDSLNPWRPATGQGLYVAYDPTLLKRGKKLEPQQEAVQH
                     LRAIAHEIFTYILLRRLLTRLKSTDVPVAMVRFQPSSSTDELIYAQLKTIEFALGELF
                     PITSQGLSLEKDNTYVYRRTAAAWGMLTDFPALLTTGIRPTIDRIAVIAFTGRPTDRR
                     IGGTGPIISPDDGHALFGEVILFEATGKGWRITSPRRFIHLGNRRDNLSTMRAIQSEL
                     EQVRDAGFRKVLCIAREFQSPRFGLSEEDRRPYLTPSVFDNLASELSDCDVYPLVKGA
                     YEGVRVGKLESFETLILKGAPLRAEISTAVVPVLTVANLRTVGTKRRTTHSSCSSYYI
                     ATGAYSDPERRARLTSELLSKSPLQETLLSVLLGLHFFRYEKEPYLSQGKLVIGSKLD
                     PFSTLTGQPNVGQHSEILKLPVSRRHRVTVAMLPILKTVGDVLDIIQ"
BASE COUNT         1742 a         1518 c         1442 g         1661 t
ORIGIN      
        1 gccaaaagaa aagccctccc cagggggagg gttgggtggc ggaggacgat agctgctgga
       61 cgcgtaatac gcggctggta tccccatttc ttaaaggcta tagggatagc ctttaacctg
      121 gattttcagt ttgttcctgt tgcctgagca aaaaaatgtt caagagttga aattcgtggg
      181 ttgcattgaa ggcacttggt gaaaagatct gctcacgaaa catttctact ccatttaatc
      241 gaaatactcc gaagggaata tcatcatatg cagccacaac gggggcaatt cgttgtgcct
      301 taccaaattg aatccgagtc aattcactaa tgagatcata ccattcacgg tgaatctctc
      361 gactggaaat gctgaaatgc tccgaccagc ggctcacttt ggaaaaacag agtgcaggct
      421 gagcaagcca gactccttct cgatggcgat gttcaaggcg ctgacgatct tgcgttatat
      481 ctcctgacgc ctttgaataa aacaaactat caagtaaatc caccattcgt gcagcaccgc
      541 tcataagctg gcgtgaccgg atatcaagtt tagttgtgaa gccatccttg cttgttaaaa
      601 gacctgttcg gagagcctga agaagtcctt ttagttgtct tgttgttgct cgactttcct
      661 ctcgatttag gataatcaat tcatcgctca tttctccaac tcgttcttca acgagcaatc
      721 ctgagcaata catggcttca actggaaatt ctggaattgt cacaatccca tcaaggccat
      781 tgcgaatgct tggaacaaga ctccgttcaa tccaatcacg ccggaatatt tcggcatcta
      841 atctggttga ctgtgattgc tttgaaatat tgatgatacg atggtttccg tgagtacgaa
      901 acatcatgca catggcatta tgtgttagtt gacagactga tcgtaatgcc gaatttgaag
      961 tagcatggag ttctttttcc aattcaccca aaaagttgat aatttcttcg gtatcggttt
     1021 ggtcgctgat ttcaacgagt ttaactccaa caggcacaac agaaacagcc tggtcaccaa
     1081 tataccctac tccacaaatc cgggtcatca ctgcagctcg caacagtaca cacatggcaa
     1141 ggagatcatt gatccgctcc tccgagtttg aaggtccaag cgtttccgtc aaaaagaatt
     1201 caattcgacg atcaacatag tctccattga ttatatgtcc atctttacaa tgagcggtac
     1261 cacgtccgcg ataaatgagc tggactattt ccattagtga ttgctcagga gcaaagcggg
     1321 ggacttcggc caaaataacg gtggtgagag ggaaagaaat accacgggat gccgacgaag
     1381 ttatgagaat gacgcgtgct gacaaatggt gtttctcctc gatgattcgc gcacgctctg
     1441 aattcctgat ccgagaattg attttgagaa tatcaggtcc cttgaaacca caggcagatt
     1501 ttagttcctc agccaaatca tcaaggcggc gaatatcctg gacgaaaacg ataatctgcc
     1561 gggccccctc tgaaagggct gtaatcacag cttcaaccag gcgatcattc aaaattgggg
     1621 ttaactcagt tggttttgct tcaactcgcc catggtcacc aatttgtgct ggtacaaggg
     1681 tgagatgata gttaagtgtc agtgatcgag ccgggaaact cgatgcattc aaaagtggta
     1741 ctgcttgacc gcaaaaatgg aaactaccaa gtgagattgg atttgatgat ccggcaggtg
     1801 aaacaataat ttttggaggt tcagggcggg tctcggccag aaagcgttcg gcaacccgct
     1861 cgtcggttaa tgaggcgtca gcaacaataa cacgaatgcg aaaggggctt ttccggtttg
     1921 cctcgtcata cggagttaca aaccattgat caaggcggct aacgatgtcg tgtagcaaca
     1981 tcaccccttc ggatgaaccc gtgagctcat ctagcataac aactatctca gggattctgg
     2041 ctgcaaattg atcaagtcct ccaagccatg gtgatttgtt tttttgagga acaattgcgc
     2101 ccagagcatc aatggtcttt ttcccattca ccactgcttt caatgcttga gttgaaacag
     2161 cggcaacaat cctatcaatt ccagtttttc tggcacattc aatgccagca gaaagacaag
     2221 ccagaacgcc ttttgcatct gatgaccgtg gcacaatggt atcaactgat tgttctcgat
     2281 accttcgctc tcggtttaca gcaggcccgg cttcgatttc acgttcgact ttgtcacgaa
     2341 ccaaacgaac atttttgaat tcttccggaa ggcacacacc acaaccacca tacacaaccg
     2401 cacccgaaat tggctgtaac tcacgatgat ggcggcgttc cgcttcacga atttgaataa
     2461 actcgtcaat tgtggcagca ttggtcgtta gtgttacaat gggtggctca gattgatttt
     2521 caaagttccc taaaatatcc cgattgatct gaatacgggg gctgaagtag aggaaaagaa
     2581 aaccatgtgg ccgcatcaaa agactgttac gaatggctgt tgttttcccg attcctggat
     2641 gcccagtcag agcaatgaca gggatccggg catccgggta atcacgatca agcaaagcct
     2701 gaacgaacaa ggcatgggca tcccgcaagg ttggattttg atacaatttg agagacttct
     2761 gaatttgctt ttgctggtca ggaatccaat tttgaatctc aggtgttgta agccattcct
     2821 gaatttgttc tagagttact ggttctgccg gattatgagt ctgtgtgatt ggctcttgaa
     2881 tctctgcagt gagtagtgtt gcgctctcta aacgtgacaa agcaacctct ctcagcttcc
     2941 ggatttcttt tcgtgcctca attggaaaac ttgaaaccag agttcgaaat gcaactttgc
     3001 gctgtcggga ttggacctcc tcccaacttt ctccttttcc agccgtttga ttccagataa
     3061 tttctgcaaa aggtcttagc cgattgcggt gttcaaattg agctgcgata gactgtgttc
     3121 cccggtcagt tacggcaatt acttgaacca ggcctttttt catttcaaat ccgacctcag
     3181 ccaagcggtc aagaaagtca gtggcatagg cgcacccttg aaataatttt tttgcaggct
     3241 tatcggcccg acgaatacca tcaaggtaat ttgacatttc tgaactgact gcaatgtcgt
     3301 tttctgtcaa atcaagattt aattgagaat aggcaccacg acgacgaagc aacccgacaa
     3361 cactccgaaa gtgattgaga acagcggctg gatcacctgg atcaggcagg tgatgagcgc
     3421 tattggctgc atagagggag aattctaaaa ccagtacatg ccaccttctc cctttcttga
     3481 tgaggctaat ccagtcagca tcgcgaaatc gccctttccc tgccggacca ttcgcaagca
     3541 atacagattt ttcagccggt agattcaact tctgaaccag tgtattgaaa gcctgctgtc
     3601 tggattcttt ttcaagttga gctaacccaa gatgatggct gccaagtgga caaaacaacc
     3661 attgaagaca tacttccccc gatgatccgt caccaatgac gtttttcaaa tagtcacgaa
     3721 tcaatccata cccagcaatc gaggcccagc cagccatgtg ttccagatat cgctcaagac
     3781 gggatagttc accctgatca gtaattccaa gctcgctaaa caaagcaacc agacgattta
     3841 gggaaacata tcgaaaatca ctgattgatt tttggggaag ttcaagcctg gcattttgta
     3901 atgcacgggc acagccagct tttacaaagt gctcaaaggc atgaccaagc acggtagcaa
     3961 gagtaacgtc agaatccagc atgagacaga tccctccggc tattgaataa tatccaacac
     4021 atcacctacc gttttgagaa tcggaagcat cgcaacggta acacgatggc gacgtgaaac
     4081 cggaagtttc agaatctcgg agtgctgccc aacgttgggt tgaccagtta aggttgaaaa
     4141 tgggtccaat ttacttccga tgacaagttt tccctgagat aagtagggtt ccttttcata
     4201 cctgaaaaaa tggagtccaa gcagtacaga aagcagggtt tcctggagtg gactttttga
     4261 aagcagctcc gacgtaagtc gggcacgacg ctcagggtcg ctgtacgcac cagttgcgat
     4321 gtaatagctg gaacaacttg aatgtgtagt ccggcgcttg gtaccaactg tccgcaagtt
     4381 tgccacggtc aaaaccggca ccacggctgt agaaatttct gcccggagag gtgccccttt
     4441 gagaatcaaa gtttcgaaag actccagttt cccaacccgt accccttcat aggctccttt
     4501 caccagggga tacacatcac agtcactaag ttcagaggca agattgtcaa acacagatgg
     4561 agtcagatat ggccgtcgat cttcctcgga tagtccaaac ctgggggact gaaattcacg
     4621 tgcaatgcag agaactttcc tgaatccggc atctcgaacc tgttcaagct cagattgaat
     4681 cgcgcgcatt gtactcaaat tgtcacgtcg attccctaga tggataaaac gtcgtggact
     4741 tgtaattcgc cacccttttc cagtagcttc aaacaaaatg acttcaccaa aaagggcatg
     4801 cccgtcgtca ggtgaaatga tcggaccggt gccgccaatt cggcgatctg ttgggcgacc
     4861 tgtaaaggca atgacagcaa ttcgatcaat tgttggtcta attccagttg tcaacagagc
     4921 tgggaaatca gtgagcattc cccaagctgc ggctgtacgc cggtagacgt aggtattgtc
     4981 cttttcaaga gaaagtcctt gtgatgtaat tggaaacaat tcacccaggg caaattcaat
     5041 cgttttgagt tgggcataga tcaattcatc cgtactactg gacggctgga atcggaccat
     5101 tgcaacaggc acatcggtgg attttaagcg agtcagcagc cttcgtaaca atatgtaagt
     5161 aaatatttca tgcgcgatgg ccctcaagtg ctgtactgct tcctgttgcg gctcaagttt
     5221 ttttccacgt ttgagcagag ttggatcata tgcaacgtaa agcccctgac ccgttgccgg
     5281 acgccaggga ttaagagaat ctaaatactc ccttccggga tagataacca ggtagactac
     5341 cttttccggc agttcccgtt gaagaatata ccgattgata ctctctttat tcgccctcga
     5401 cacccatctt tccgaaaagt gcacagtaat cggaaaactg gcaaacatac catttggggc
     5461 ttcctgagtt acagtcaaat gtgaaaggta cccgacttct tctcgatcag gtggaaacga
     5521 tagaaatggc tttgcctgtt taatcccaac tcgatccagg attgatggta ataaacagag
     5581 gtacagatca aatggttgac ggacaagcgt accaagaaca tcctttcgtt ctttcaacaa
     5641 tgtatgtaat gaagtggcga tgatgttcac cttctgggtg cgggaacgta actcagaaag
     5701 accttctcgg actccatccc gaccgagtcc aagaagttgc ttcaagttca taaagcgatc
     5761 ttgcaggctc aatgaacgac catcgggaag acaaagccag agtgttgcca gattgcacat
     5821 agcttttctg gccaagaaac ggcgtgatcc ctcatcctcc tcgtttccca atgaaagagc
     5881 acgttcgatt tgatcaaggc gatattcaag taccgactca aatcttcttt cttcgctcag
     5941 agcaattggg ccatttaaaa tcacatgata gctgtttgca attgattggg tttcgcgtgg
     6001 gccaaagact tcatcggtta taaaccctga tgaaataatt cggatcggca aactatcaaa
     6061 tgctgaggta agcatcattt cctgtcgaaa agaaaatcgg tcagcaaccc caaaaacacc
     6121 cattgaaagt tcaagatcct gatcctccaa tccctcggcc tctccgaatt ttcccaatgc
     6181 agaaactcct gccaggtact gtgtcagttc gggaatcttt ttgtttgatg tagctgcctc
     6241 acacagcgcc ttcaacaaca taagtccaat ttcccgttca actcgcccca ttcctttttc
     6301 acggattgtc tgaaccagtg catgcagtcg tgttggatca gcatcggtac gttgtcggtg
     6361 acg
//
LOCUS       DAOQDO010000047         6301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7406_length_6301_cov_3.031060, whole genome shotgun sequence.
ACCESSION   DAOQDO010000047 DAOQDO010000000
VERSION     DAOQDO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7406_length_6301_cov_3.031060"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="groEL"
                     /locus_tag="PKZ53_01400"
     CDS             <1..1021
                     /gene="groEL"
                     /locus_tag="PKZ53_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNJ39118.1"
                     /translation="VHQLVVGPIDPNALILLNEKKISSMKDLLPLLEQVAKLGKPLLI
                     IAEDVEGEALATLVVNKLRGTLQVSAVKAPGFGDRRKAMLEDIAILTGGKVISEDLGI
                     KLENVKLEDLGRAKRVTVDKDNTTIVEGAGKDKDIKARVEQIRAQIENTTSDYDREKL
                     QERLAKLVGGVAVIKVGAATETELKEKKARVEDAMHATRAAVEEGIVPGGGVTLLRAQ
                     QALDGFKVDGDEEERFGVQIVRRALEEPLRQIVANSGQEGAVVVSKVLGSEVESFGFN
                     AATEQYEDLVQAGIIDPAKVVRTALQNAASIAGLMLTTEALITELPEDKKAAPAGMPG
                     GMGEY"
     gene            complement(1437..1691)
                     /gene="rpsR"
                     /locus_tag="PKZ53_01405"
     CDS             complement(1437..1691)
                     /gene="rpsR"
                     /locus_tag="PKZ53_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNJ39119.1"
                     /translation="MEEQSSLRKPSGSRLRGPRRKKRCPLLEDKIDYIDYKDVKLLKQ
                     FVSENQKILPRRLTGVSAAMQRRLAVAIKRAKQVALLGYK"
     gene            complement(1709..2818)
                     /locus_tag="PKZ53_01410"
     CDS             complement(1709..2818)
                     /locus_tag="PKZ53_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39120.1"
                     /translation="MSNGKNRKPRRKESGAKQPVNVAQMLQQGQKALEAGRPDEALEA
                     FVTALELDPGSAEAHFHFGALLLDSEEFEIGIEHLQEAQRLAPQDVRPLLLLGEFYLE
                     EDELEEAELYFRTAIGIDPQNSFCFHYLGNLLLELGRYDEAHSSYEKAVALNPTEAVH
                     HLGLGSVYADQERFDEALAKFQHALTLAKDDWRIHHDMADLLVAMGRPEEALPHYRKT
                     LELDTYNVDARYGLGNALLLTNDSAGAVDAFKQSLEDGLEAPLAYIKFGIALTLQHRA
                     EEAREAFLEALNLLEEEQEEGHAPETHYELIIAELGSGNSTNAQDIAEQLITHGNPDP
                     AALSELLTDLKLLVQLPGAQDVIHILSRQADQFVN"
     gene            complement(2858..3895)
                     /locus_tag="PKZ53_01415"
     CDS             complement(2858..3895)
                     /locus_tag="PKZ53_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ39121.1"
                     /translation="MLSSASPLQPIPTYILSGFLGSGKTTLLIELLRFSRFHGRKVAV
                     LMNEFGDINIDGELLTGEGFSVIELSDGCVCCQIGEDFIQAFTEVARRSPEMIFVEAT
                     GLADPVELLDQATMPHLLPLIHVTKLVTVADPKNFPRLSKVLEKIVRRQVQFADAVFI
                     SKTDEATPDQIAEIQQTIARLNPHAPVWAGIQGHVPDDADYRWLFTEEASETLTAKRA
                     AMRASVDALSDEEYQTHRDFHTLSCRLVRPLEREKFETFLRHLPPEILRAKGFIRFVN
                     DPQLWVVMFVNGDIYLRPAQLSPAPEEHLVFIGSMLDHARIASELTACEAGRRSLSLA
                     ERLTPSTVTEA"
     gene            4234..4929
                     /locus_tag="PKZ53_01420"
     CDS             4234..4929
                     /locus_tag="PKZ53_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ39122.1"
                     /translation="MTESDPKYLQQAREYFEAGDFDAALAELYALLWSNINDMEIHLL
                     LWQVMEGKLAEQSRLDRLREAVHAPIVKPKIEPPEAAQPTVFPQAAPFSRPREATRFV
                     VDKPIILVGHDNRNRFFAELATVENLSSKGATLLTHRSLALRDQVHLFSPSGQEEEYV
                     IALVRNLRKSHEDGRHRLGVEFLQKPGKWLFSDELIEAARTADDKDDQESTLNSEAQA
                     ESESTAPSASSQA"
     gene            5022..5918
                     /gene="ispE"
                     /locus_tag="PKZ53_01425"
     CDS             5022..5918
                     /gene="ispE"
                     /locus_tag="PKZ53_01425"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNJ39123.1"
                     /translation="MTVFTYPAFAKINLFLKVLGRRPDGYHELLTLFQTVSLADRLSF
                     ERLDTPDVQLTCSRPDLAVDESNLVLRAARELQRATGCRAGARIHLEKNIPLGAGLGG
                     GSSDAATTLMALQHLWQVSLPTAELHTIAAGLGADVPFFLVGGTAAGTGRGDLIEPVP
                     DFEWPHLLLANPGVEVPTGKIFRLLKVELTSPDSVRILPAYFFQPPSFLPSLYNTLEE
                     IVFQEFPIVARVWQEMNGSGALSARMSGSGATVFGVFPDRETLETAHRQLQAHHWHLW
                     PCQTINRAEFQAFFSHPQISER"
     gene            5945..6018
                     /locus_tag="PKZ53_01430"
     tRNA            5945..6018
                     /locus_tag="PKZ53_01430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5977..5979,aa:Gln,seq:ttg)
     gene            6146..>6301
                     /locus_tag="PKZ53_01435"
     CDS             6146..>6301
                     /locus_tag="PKZ53_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNJ39124.1"
                     /translation="MRKCLTGDVATVSNLRVFSGNANRPMAEEICRHLGITLGEANTS
                     RFSDGEFN"
BASE COUNT         1430 a         1638 c         1728 g         1505 t
ORIGIN      
        1 agttcaccag ctcgtcgtag gtccgataga ccccaacgcg ctgatcctgc tcaacgaaaa
       61 gaaaatcagc tcgatgaaag acctgctgcc gctgctcgaa caggtcgcca aactcggcaa
      121 gcccttgctg attatcgctg aagacgttga aggcgaagcc ctggcgacgc tggttgtcaa
      181 caaactgcgc ggtacgttgc aggtttccgc cgtcaaagcg cccggcttcg gtgaccgccg
      241 caaagctatg ctcgaagaca tcgccatcct gaccggtggc aaagtcatca gcgaagacct
      301 cggcatcaag ctcgaaaacg tcaagctgga agacctgggc cgcgccaagc gcgtgacggt
      361 tgacaaagac aacaccacca tcgtcgaagg cgctggcaaa gacaaagaca tcaaagcccg
      421 tgttgagcag attcgggctc agatcgaaaa cacgacctct gactatgatc gcgaaaaact
      481 gcaagaacgg ctcgcgaaac tggtcggcgg tgtggcggtg atcaaagtgg gtgccgcgac
      541 ggaaaccgaa ctgaaagaaa agaaagcccg cgttgaagat gccatgcacg cgacccgtgc
      601 ggcggttgaa gaaggcattg ttcctggcgg cggcgtgacc ctgctccgcg ctcaacaggc
      661 cctcgacggc ttcaaagttg atggtgacga agaagaacgc tttggggtgc agattgtgcg
      721 ccgggcgctc gaagaaccac tccgtcaaat cgtggccaac tccggccagg aaggtgcggt
      781 tgtggtgagc aaagtactgg gcagcgaagt tgaatcattt ggcttcaatg ccgctaccga
      841 acagtatgaa gatctggtgc aggccggaat cattgatcca gccaaggtgg tgcgtacggc
      901 gttgcagaat gccgcgtcaa tcgctggctt gatgttgaca accgaagcgc tgatcactga
      961 attgcctgaa gataagaagg ctgctccagc gggtatgccc ggcggcatgg gtgaatacta
     1021 aacagcccag ggcttagggc ttagggcttg gggctgaaga aatttgggtc agtatcacct
     1081 gaactctgcc gtcctcagtt tttagccttt agatatcaaa aaggcagcgt ctttctggac
     1141 gctgcctttt ttctttgggc gatagtgagc tgtgaccaaa gaaaaagcct ccttgcggag
     1201 gctttcaagt gaatcgcggt ctcaggcgag ttgcgacacc aatatggcct ggacgaaccc
     1261 ggaaaccgga atgaagaacc tcttcgcttc cgatcttttt cgactcgcca ctcgcttttt
     1321 tcgaccagtt cagcgtatct agggcggaat gaaatctttg gggttgccag gagccaaatg
     1381 attgaaagct tgtctttccc caagccccca gccctcagcc ccaagcccac aatgattcac
     1441 ttatagccca ataacgcaac ctgtttggcg cgcttgatgg cgactgccag ccgacgttgc
     1501 atcgcagcgc tgacaccggt cagacggcgg ggcaggattt tctggttttc gctcacaaac
     1561 tgcttgagca atttcacatc tttgtaatcg atgtaatcaa ttttatcctc cagtaatggg
     1621 cagcgctttt tgcggcgtgg gccgcgcaac cgactaccgg acggtttgcg aagtgatgat
     1681 tgttcttcca tggggtctca ccttcctctt aatttacaaa ttggtcagct tggcggctga
     1741 gaatatgaat gacgtcctgg gctccaggta attgaaccag caatttcaaa tcggttagca
     1801 attctgacag ggcagccggg tccggattac catgggtaat cagttgttcg gcaatatctt
     1861 gggcgttggt gctgtttccg ctgcccaact cggcaatgat caattcatag tgggtttcgg
     1921 gagcgtggcc ttcttcctgc tcttcttcaa gcaggtttaa tgcttccaga aaagcctcac
     1981 gcgcttcttc agctcggtgt tggagcgtga gggcaatccc aaatttaatg taggccagtg
     2041 gggcttcgag gccatcttcc aggctttgtt taaaggcatc aacggccccg gcgctgtcgt
     2101 tggtcaagag cagggcattg cccagaccat agcgggcatc aacgttgtaa gtatcaagtt
     2161 caagggtttt gcgatagtgc ggcagggctt cttccgggcg tcccatcgcc accagcaaat
     2221 cagccatatc gtgatggata cgccagtcgt ctttggccag tgtcagggca tgctggaatt
     2281 tcgccagcgc ttcgtcaaag cgttcctgat cggcatacac actgcccaat ccaaggtggt
     2341 gaacagcttc ggtcggattg agggccacag ccttttcata gctggagtgg gcttcgtcat
     2401 agcgtcccag ttcgagcaac aggttgccaa gatagtggaa gcagaatgaa ttctgcggat
     2461 caattccaat ggccgtccga aagtacagtt cggcttcctc cagctcatct tcttccagat
     2521 agaattcgcc gagcaggagc agtggtcgga catcctgggg ggccaggcgc tgagcttcct
     2581 gcaggtgttc gatgccgatt tcaaattctt ctgaatcaag caagagcgcg ccaaagtgaa
     2641 agtgggcttc agccgagcct ggatccagtt caagcgccgt cacaaaagct tccagggctt
     2701 catccgggcg gccagcttcc agggcttttt ggccttgctg gagcatttgg gctacgttca
     2761 caggttgttt ggcacctgat tctttgcgtc gtggttttcg gtttttgccg tttgacatac
     2821 aattccttct cttcaatctg tcttctacaa taggaaatca tgcttctgtg actgtggacg
     2881 gtgtcaggcg ctcagccagt gacaatgacc gtcgcccagc ctcacaggcg gtgagttcag
     2941 aagcaatgcg tgcgtgatcc agcatcgatc caatgaaaac caaatgctct tcgggggctg
     3001 gcgagagctg cgcgggtcgc aggtaaatat ctccgtttac aaacatcaca acccacagtt
     3061 gagggtcatt gacaaaacga ataaatcctt tggcacgcaa gatttcgggg ggcagatgcc
     3121 gtaaaaatgt ttcaaatttc tcgcgctcaa gtgggcggac cagccggcat gagagcgtgt
     3181 ggaaatcgcg atgggtttgg tattcttcgt cgctcagcgc atcaaccgaa gccctcatcg
     3241 cagcccgttt ggcggtgagc gtttcactgg cttcttcggt aaataaccag cgatagtcag
     3301 catcatctgg gacgtgtccc tgaatgccag cccacaccgg ggcatgtggg ttgagtcggg
     3361 caatggtttg ttggatttca gcgatctggt ccggtgtcgc ttcatcggtt ttgctgataa
     3421 ataccgcatc ggcaaactgg acctgccggc gcacaatctt ttccagaacc tttgaaagcc
     3481 gtggaaaatt tttgggatcc gcaaccgtca ccagtttggt cacgtggatc aacggcagca
     3541 agtggggcat ggtggcctga tcgaggagtt caaccgggtc agccaacccg gtggcttcca
     3601 caaaaatcat ttccggggat cgccgggcca cttcggtaaa tgcctgaatg aaatcttcgc
     3661 caatctggca gcacacacag ccgtcgctga gttcaatcac gctaaaccct tcgccggtga
     3721 gcaactcgcc gtcaatattg atatcgccaa attcattcat caggacggca actttgcggc
     3781 catgaaagcg ggaaaacctg agcaattcaa tcagcaacgt ggtttttcca ctgcccagga
     3841 atcctgacag gatataggtt ggtattggct gaagtgggga cgcggatgac aacatcaaca
     3901 aaaaaatacc ggcactgtcg tggtgatcag ggcactttga acgtctgaaa atgaatagcg
     3961 aatccggtca ggaatgaccg ttttttacga aagctataat cgtaacattg aatcaggaaa
     4021 attctcaacc cctaagggct tagtttcaga atagatggtt tcagcaggtg ggtttgggaa
     4081 aagactgttt ttccggattc agatatggta tctgttttca accgaaagac ttccgtgtaa
     4141 tagatctctg gcatctgtgt ttttgcccat catgggtcac cttttttcaa ctcagtacta
     4201 ttcttcaatt tttattctgg catctggctc tccatgactg aatctgatcc aaaatatctt
     4261 caacaggccc gtgaatactt tgaagccggc gattttgatg ccgccctggc agagttgtat
     4321 gcgttgctct ggagcaacat caacgacatg gagattcacc ttttgctctg gcaggtcatg
     4381 gagggcaaac tggctgagca atcacggctg gatcggttgc gggaagcggt tcacgcccca
     4441 attgtcaagc ccaagattga gcctcctgag gcggcgcaac cgaccgtctt tccgcaggcg
     4501 gcgccctttt cccgccctcg cgaagcaacg cggtttgtgg ttgataaacc gattattctg
     4561 gtgggtcatg acaaccggaa ccgtttcttt gctgaactgg ccacggttga aaacctcagt
     4621 tcgaaaggtg ccacgctctt gacccatcgc agtcttgcct tgcgtgatca ggtacatttg
     4681 ttttcaccaa gcgggcagga ggaagagtat gtgattgccc tggttcgcaa tcttcggaaa
     4741 agtcacgagg atggccggca tcgactgggg gttgaattcc tccaaaagcc gggcaagtgg
     4801 ctgttttcag atgagttgat cgaagccgcc cggaccgctg acgacaaaga cgaccaagaa
     4861 tctacactca acagcgaagc tcaggctgaa tcagaatcta cggcgccgtc ggcttcgtcc
     4921 caggcataaa gctgacccac tgacactgtg accgggtgac cagatgatgt acgggcgcgg
     4981 ttgttttgaa tcaggcgttc cttcaccctc cgaattctcc aatgactgtt tttacttacc
     5041 cggcatttgc caaaatcaat ctttttctca aagttcttgg acgtcgccca gatggttacc
     5101 atgaacttct cacgctgttt cagacggttt cactggctga tcgcctttct tttgaacgac
     5161 tcgatacgcc tgacgtgcag cttacgtgct cacgaccgga tctggcggtg gatgagagca
     5221 atctggtgct gcgggccgca cgggaacttc agcgggcaac tgggtgtcgt gccggggcgc
     5281 gcattcacct ggaaaaaaat attccactag gggcggggct tggcggcgga agcagcgatg
     5341 cggccacgac attgatggca ttgcagcacc tctggcaggt gtcgctccct accgctgagt
     5401 tgcacacgat agccgccggg ctgggggcag atgttccttt tttcctggtt ggcggaacgg
     5461 ctgccggcac cggtcgtggg gatctgattg agccggtgcc tgattttgaa tggccgcatc
     5521 tgttactggc caacccgggt gttgaagttc ccacggggaa gatctttcgc cttttgaaag
     5581 tcgagttgac aagcccagac tcagtacgta tactgcccgc ttactttttt cagcccccat
     5641 ccttcctgcc gtctctgtac aacacacttg aggaaatcgt ttttcaggag ttcccgattg
     5701 tggcccgagt ctggcaggaa atgaacggtt caggtgcgct cagtgcccgg atgtcaggaa
     5761 gcggggcgac tgtttttggg gttttcccag atcgggaaac tttggagaca gcgcatcgac
     5821 agttacaagc acaccactgg cacctgtggc cttgtcagac catcaaccgc gctgagtttc
     5881 aagccttttt ctcccatccc caaatttcag aacgctgaaa gaagtactgg tttgccttct
     5941 ttgctggcgg gtcgtccaat ggtaggacat gcggctttgg accgtagaat cggggttcga
     6001 ctccctgccc gccagccaat ccggcacaag tcatcaggac acaagggaaa agcgcccaga
     6061 gcgctttgac tttgtggctt tgtgtgcctt gtttccggaa aatgaccgca tctgacgaca
     6121 agcaaaccgg atcgcttgca cgtcaatgcg aaaatgtctc acgggggacg tagcaaccgt
     6181 gtcgaatctt cgggtttttt ctggaaatgc caatcggccc atggccgagg aaatctgtcg
     6241 tcacctgggg attaccttgg gtgaagccaa cacctcgcgc ttcagcgatg gggaattcaa
     6301 t
//
LOCUS       DAOQDO010000048         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7466_length_6268_cov_3.089812, whole genome shotgun sequence.
ACCESSION   DAOQDO010000048 DAOQDO010000000
VERSION     DAOQDO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_6268_cov_3.089812"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1432)
                     /locus_tag="PKZ53_01440"
     CDS             complement(92..1432)
                     /locus_tag="PKZ53_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39125.1"
                     /translation="MSSAVPSPYDLRKYLEQHRLRADQMLNLASPALNGGLPTNQHWE
                     WLNVLLTRAFSPDAKTGVGIRAKDEVTLINSITPLIVRPKFVDYLEKESILDRSGSLL
                     KQVQRTSTFDELVRNQLPAKRALHSLFHQNSTSPELLTRNVQLNIEMHGKFEKLYGEV
                     IKQMQDLSEQLRQLRVNPTTLPEESSETSVVAAMEFAVKPAPWVTGDYSKNIANLQTV
                     IRMRDMLQRAVIYFGMSLPSVNPTAPVTLADPAAFLHSTPTPLPGPPTRTPAPAFTIQ
                     PTPLPQGADPNLAHELETEIQACINYLTQTMANSGTSSSTVRVIRLTTGSFLLTSWEA
                     AAFEPTANVPEWQVSYLVREGRKLLQRSIALLAVIEQRRPKPTENIKLTPGQRSAAQY
                     YLGQITEVLNTFDRFLQRSATSATDIEILSNLRNTRRKIVMTQTQLKQTIGIHS"
     gene            complement(1732..2409)
                     /locus_tag="PKZ53_01445"
     CDS             complement(1732..2409)
                     /locus_tag="PKZ53_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ39126.1"
                     /translation="MQNRYYRLPNAFHISLDPLLDELRQFFVLNGHQVQIIPINNGHI
                     LQAQKESTLSNLTAQSSALTVRLTREIDGLRVEVGNGKWLDKAAIGLVGYVLLAPLIL
                     LPIVGAINQFKLSEDVWEKIEAFVARQNGAPAPGTAYSSPPPPAGYTSPTYYPPPASA
                     VEVRCASCKTVLATGAMFCSRCGTRVGNGSVVDSFASSSPTCPDCGLMNPLGARFCSG
                     CGHRFGG"
     gene            2639..3523
                     /locus_tag="PKZ53_01450"
     CDS             2639..3523
                     /locus_tag="PKZ53_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39127.1"
                     /translation="MKIHKLHARLVSLSLAASLWVAGSALVTRGTPLVDPTRTPDCPG
                     VATMDCTGVPELICKTACNQHQSLVIRDRCKYRQRLHMERYEKPKAPGLPPGERKQFR
                     DTEVEIGYQVDENDKTNYLVVPRVVADTDDKGVPKTKVNPKESTGLTSQAFLDLIFFP
                     LLPEKLPYYEFEPAPAEREGERAFRFKPKANIKSVPLAVGIVYVNGTTGDMLTVQVEA
                     MYNLQVLDKHLKDIESISATVDYSEFNGRFRMPTLARGEGISNVTKFKGYFKFNFEES
                     AYVPALTLPGWSGISTNN"
     gene            3783..3859
                     /locus_tag="PKZ53_01455"
     tRNA            3783..3859
                     /locus_tag="PKZ53_01455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3817..3819,aa:Pro,seq:cgg)
     gene            3942..4220
                     /locus_tag="PKZ53_01460"
     CDS             3942..4220
                     /locus_tag="PKZ53_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNJ39128.1"
                     /translation="MAVVARRFVITGRVQGVGYRYYTLRAACQYQIRGWVRNLPDGSV
                     EAWVEGTLDSIEKLKHDLAFGPMYAEVRKISETAVEPTGHFDTFRING"
     gene            4233..4754
                     /locus_tag="PKZ53_01465"
     CDS             4233..4754
                     /locus_tag="PKZ53_01465"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNJ39129.1"
                     /translation="MDALRKLVREIPDFPKPGILFYDITTLLKDPKGLKRVINRMSEG
                     FAGEKIDVVVGIEARGFIFAPAIAYHLGAGFVPVRKPKKLPAETERECYDLEYGSDCL
                     EIHKDAIQPGQRVLIADDLLATGGTALAVIKLVERLGGTIAGVTFVIELDFLNGREKL
                     AGYHIESLLRYDS"
     gene            4848..4923
                     /locus_tag="PKZ53_01470"
     tRNA            4848..4923
                     /locus_tag="PKZ53_01470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4881..4883,aa:Arg,seq:cct)
     gene            5006..5941
                     /locus_tag="PKZ53_01475"
     CDS             5006..5941
                     /locus_tag="PKZ53_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ39130.1"
                     /translation="MEKVDVSILISTRNRASVLRHTLQSLRNQHLNGIRWEIVVVDNG
                     SSDDTAQVLHEAQRWLPLIPLTEPTPGKNRALNRALDVVTGELLIFTDDDTEHTEQWV
                     SELVAASKQWPEVSIFGGPVIPLLPPETPEWIRQNRRITSIAFSHFDLNHTENTRLED
                     PTLPFGPNYALRAAAVDGKRFSLRLGPQAADPQSPLGDETEFLARIYEENQQIGYVPR
                     AKVFHRVEKHQLESAWLFNRAFRFGRGFVHFAPNTTSFRLRGVPARLIVVFCLTWLFH
                     HLFFFRAEPQKFKTSSTLHFLRGQIFEYRQLHRSG"
     gene            6052..>6268
                     /locus_tag="PKZ53_01480"
     CDS             6052..>6268
                     /locus_tag="PKZ53_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ39131.1"
                     /translation="MTYPQLELSVLIATRNRARLLGPTLASLARQEMNGLRWEVIVID
                     NNSTDDTPQIVEQARRTLPLVLLFESEP"
BASE COUNT         1527 a         1526 c         1663 g         1552 t
ORIGIN      
        1 agaaccaaga accaagaacc aagaaccaag aaccaagaac caagaaccag gcactcacca
       61 cactcttcat tcttcattct tcattcttca ttcacgaatg aattccaatc gtttgcttga
      121 gttgagtttg ggtcatgaca attttgcggc gggtgttgcg caggtttgag aggatttcaa
      181 tatcggtggc tgaggtggct gagcgttgca aaaaccggtc gaaggtattg aggacttcgg
      241 tgatttgacc aaggtaatat tgcgcggcac tccgttgacc aggggttaac ttgatgtttt
      301 cagttggctt ggggcgacgc tgttcaatta cggccagcaa ggcgatgctg cgttgcaaga
      361 gttttcgccc ttcgcggacg aggtaactta cctgccattc tgggacattg gctgtgggtt
      421 caaatgcggc agcttcccat gacgtcagca aaaagctccc ggtcgtcagc cgaatgaccc
      481 gaaccgttga cgaactcgtg cctgaatttg ccatcgtttg ggtgagatag ttgatacagg
      541 cttgaatttc ggtttctaac tcgtgggcca ggtttgggtc tgcaccctgg ggcaacggcg
      601 tgggctggat ggtaaacgcg ggggctggtg tccgagttgg agggcctggg agcggggttg
      661 gggttgagtg aagaaaggcg gctggatcag ccagcgtgac cggtgccgtt gggttcaccg
      721 atggaagact cattccaaag taaataaccg cccgttggag catgtcccgc atccggatga
      781 ctgtctgtag attcgcaata tttttgctgt agtcacccgt gacccagggt gccggtttga
      841 cggcaaattc catcgccgcg acaactgaag tttctgaaga ttcttcgggg agtgtggttg
      901 gattgacccg aagctggcga agttgctcgg aaagatcctg catctgtttg atgacttcgc
      961 cataaagctt ttcaaacttg ccgtgcattt caatattgag ctgcacgttt cgggtcaaaa
     1021 gctcaggtga agtgctgttt tggtgaaaca gtgaatgcag tgcacgtttg gccggtagct
     1081 gatttcgaac cagttcatca aaggtgctgg tgcgttgaac ctgtttgagc aagcttccac
     1141 ttcgatccag aatggattct ttttccagat aatcaacaaa tttaggccgg acgatgagtg
     1201 gggtgataga gttgatgagt gtgacttcgt ctttggcacg gatgccaacc ccagttttgg
     1261 catcgggtga aaaggccctg gtcagtaaaa cgtttaacca ctcccagtgc tggttggtcg
     1321 gcagtccccc attgagcgcc gggctggcca aattcaacat ttggtcggct cgcaaccggt
     1381 gttgctccaa atatttccgc aaatcatagg gtgacggaac agcactgctc ataaaaagtc
     1441 acaacgccgc acgggaaaaa ttgcaaccgg catcaatggt tgcaatgcct gataaaccct
     1501 gactggagtg aaaccaaaaa actgccaacc cctggaatgg aatctgattg ttcggtacaa
     1561 ggaggcattc gacaggagcg ttcaccacac ttgaagagag tgagctctct gttgatcagc
     1621 ttgggtgctt tggcctttga aaaggtttga tttcaaaagg gttcaaagca tatcgggatc
     1681 aggtgaaccc ctgtcaacag ctttgatctg gtcgtgcggc ctggggaggt gttatccacc
     1741 aaaccgatga ccacaaccag agcaaaaccg tgccccaagg gggttcatca acccacaatc
     1801 ggggcaggtt gggctggaac tggcaaacga gtcaaccact gagccattcc caaccctggt
     1861 tccacatcgt gaacaaaaca tggcaccagt tgccagaact gtcttacatg aggcacagcg
     1921 tacctcgaca gccgaagcag gggggggata ataagttgga gaggtatatc cagccggtgg
     1981 tgggggagag gaatatgccg ttccgggcgc tggcgcgccg ttttgccggg ctacaaacgc
     2041 ttcaattttt tcccaaacat cttctgacag cttaaactga ttgattgcgc cgacgattgg
     2101 aagcaaaatg agcggcgcca acaacacata gccaaccaat ccaatcgctg ctttatcgag
     2161 ccacttgcca ttgccaacct cgacgcgcaa cccatcaatc tcacgagtca ggcgcacggt
     2221 cagggccgaa gactgcgcgg tgagattgct cagggtcgat tctttttgag cttgcagaat
     2281 atgaccgtta ttgatcggga tgatttgaac ctgatggccg tttaagacaa aaaactgccg
     2341 caattcatcc agcaatgggt caagcgaaat atgaaaggca ttgggaagcc ggtaatatcg
     2401 gttctgcata ggcgagtggc tctgggaaaa tttaaaatac caatcaagga tgtgaagtcg
     2461 gaagccacat tgaaaagaca gccgtggagg tcgcagtcgc atcaggttta ttctcatcag
     2521 aaaacacagc cgtggccttg ctgcatcaga caatggattc ctgacagtgt tcaatttcaa
     2581 aatctcattc ttcaaacaac aaactatttc gaaactgcga gatggagtaa aagataccat
     2641 gaaaattcat aaattacatg cccggctggt ttcgctttct ctggccgcat cattatgggt
     2701 agctggctca gcgctggtta ccagagggac accactggtt gacccgactc ggacgccaga
     2761 ttgtccaggc gtggcgacga tggattgcac cggtgttccc gaattgattt gtaaaactgc
     2821 ctgcaaccag catcaatcgc tggtgatccg tgaccgatgt aaatatcggc aacggctgca
     2881 catggaacgc tatgaaaaac cgaaagcccc tggtttgcct cctggtgaac gcaagcagtt
     2941 ccgggatacg gaggttgaaa tcgggtacca ggtggatgaa aacgacaaaa ccaattacct
     3001 ggtggttcca agagtggttg ccgatactga cgataaaggc gttccgaaaa ccaaagtcaa
     3061 tccaaaagaa agcactggcc tcacatcaca ggcttttttg gatttgatct tctttcctct
     3121 cctgcctgag aaactgccgt attacgagtt tgaacctgct ccagccgaac gcgaaggcga
     3181 gcgagccttc cgcttcaaac ctaaagcgaa tatcaagagt gttccactgg ccgttggtat
     3241 tgtctatgtc aatggtacca ctggcgatat gctcacggtt caggttgaag ccatgtacaa
     3301 tctgcaagtt ctggacaagc atctcaaaga catcgaatca atcagcgcca cggttgatta
     3361 ttcagaattc aacggtcggt tccggatgcc caccctggcc cgtggggaag gcatctccaa
     3421 cgtcactaaa ttcaaagggt actttaaatt caactttgaa gaatctgcgt atgtaccagc
     3481 gctcactttg ccaggttggt cgggaattag caccaacaat taagggcttg gggctgagaa
     3541 aaccagtgct gagggcttgg ggctgaagac agcgggctgg gggcagaaga catcgggctt
     3601 ggggctgaag actcgaagac tcggggcgga agaaaacggg tttcataccc tgagcccgct
     3661 ttcttcagcc cgctttcttc agcccgaagt cttcaacccc aggtgttgag tccaggtttt
     3721 tcatttgaca agacccagac cggagagtag tatccgtccc tttccaggac attccagttt
     3781 gacggggcgt ggctcagcct ggtagagcgt tcggttcggg accgaaaggt cggaggttca
     3841 aatcctctcg ccccgaccag gttatcctaa aggaaggcca tccgttcact tcgaattgat
     3901 ggccttcttg catttttgag agtggttgag gtgttgttct catggccgtt gtggctcggc
     3961 gttttgtgat tactggccgt gtgcagggag tcgggtaccg ttattacacg cttcgcgccg
     4021 cctgtcagta tcaaatcaga ggatgggtgc gaaatttacc tgatggctca gtcgaagcct
     4081 gggttgaagg caccctcgat tccattgaaa aactcaaaca cgatctcgcc tttggtccga
     4141 tgtatgcgga agttcgaaaa atcagtgaga ccgccgtcga gccaaccgga cattttgata
     4201 catttcgcat taatggatag gaattttgct ttatggatgc tcttcgtaaa cttgttcgtg
     4261 aaatcccgga ttttcccaaa cccggcattt tgttttatga catcaccacc ctcttgaaag
     4321 atccaaaagg gctcaagcgt gtcattaacc gcatgagcga aggctttgcc ggggaaaaaa
     4381 ttgacgtggt ggttgggatc gaagcccgtg gttttatttt tgctccggcc atcgcctatc
     4441 acctgggcgc tggtttcgtt ccggttcgga aacccaaaaa gctcccagcc gaaaccgaac
     4501 gtgaatgcta tgacctggaa tatggcagcg attgtcttga aatccacaaa gatgcgattc
     4561 agcctggcca acgggttctg atcgcggatg atttactggc gacaggcgga acggccctgg
     4621 ccgtcatcaa actggttgaa cgccttggtg gcaccatcgc cggtgtgacc tttgtgattg
     4681 aactggactt tctcaacggt cgtgaaaaac tggctgggta tcacattgaa tcactgctgc
     4741 ggtatgattc atagtgccgg gctgaaaaac ctcgggctga aaaaattgga ctaaacaagt
     4801 tcgggttcaa gtgagttgag tttttcattc cgtatctttc gagatttgct cgcgtagctc
     4861 agtggataga gcaaccgcct cctaagcggt aggtcgtggg ttcgattccc gccgcgagca
     4921 ccagcaatgt gtcgttcatc atactggaga tcggttttgg ttccctcaaa accatctcca
     4981 gtcttgtttt gagggggctg atatcgtgga gaaagttgat gtcagtattt taatttccac
     5041 ccggaatcga gcgtccgttt tgcggcacac gttgcagtcg ttgcgcaatc aacacttgaa
     5101 tggaattcga tgggaaattg tggtcgttga taatggaagt agtgacgaca cggctcaggt
     5161 tttacacgaa gcccaacgct ggttgccgtt gattccgctg acagaaccca ccccagggaa
     5221 aaatcgagcc ttgaaccggg cacttgatgt ggtgacgggt gaattactga tttttactga
     5281 tgacgacact gagcacaccg aacaatgggt ctcggaactg gtcgctgcct cgaaacaatg
     5341 gccggaggtt tcaatttttg gcggtccggt tatccccttg ctgccacctg aaacgccgga
     5401 atggattcgc cagaatcggc ggattacctc gattgccttt tcccatttcg acctcaatca
     5461 caccgaaaat acccggcttg aagatccaac cctgccattt ggacccaatt atgccctgcg
     5521 ggcagccgct gttgatggaa agcgtttttc attacgtctg ggcccacagg cagcggaccc
     5581 acaatcaccc ctgggagatg agaccgagtt tttagcccgg atttatgaag aaaatcagca
     5641 aattgggtat gttcccagag caaaggtctt tcaccgggtt gaaaagcacc agcttgaatc
     5701 agcctggttg ttcaaccggg catttcggtt tggacgtggg tttgtccatt ttgcacctaa
     5761 caccacctcc tttcggttgc gtggtgtccc ggctcgactg atcgttgtct tctgccttac
     5821 ctggttgttt caccacctgt tcttctttcg ggcggaaccg caaaagttca aaaccagttc
     5881 aactcttcat ttcctgcgcg gacagatatt tgaataccgt caactccatc ggtcaggtta
     5941 atcagattca taccaggtat cgaaaccagt ttgcacttat tccatcggat gaggagaaga
     6001 tgaaagagtt gagaggattg catttccccg caaccctgaa ccctaaatgg catgacctac
     6061 cctcaacttg agctcagcgt gttgattgcc acccgtaatc gggccagact ccttggcccg
     6121 accctggctt ctcttgcccg tcaggaaatg aatggactca gatgggaagt catcgtcatt
     6181 gacaacaaca gtactgacga cacgccacag attgtggagc aggcgcgtcg caccctgcct
     6241 ctggtcttgc tttttgaatc cgaacctg
//
LOCUS       DAOQDO010000049         6232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7539_length_6232_cov_3.083536, whole genome shotgun sequence.
ACCESSION   DAOQDO010000049 DAOQDO010000000
VERSION     DAOQDO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7539_length_6232_cov_3.083536"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2582
                     /locus_tag="PKZ53_01485"
     CDS             <1..2582
                     /locus_tag="PKZ53_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ39132.1"
                     /translation="SGNYAPAEPYYLRACAIAEKIHEPVHERVGNFIYQLGLFYQLRG
                     DFDRAEELFQRTHAIWETTLGPDHWKITQSLCILASLYLDTGEYAKAEPLLNRSLALA
                     EKIGPESNYVAQTLDLLASMYRARGDYEKAEPYYQRALVLWEKARGADNPSVALHLVN
                     LGDLYRAMGEFAKAEPLYQRALVLREKGFGKEHRFVADCIFGQAELAKEKGDLAQAEA
                     LHVKVLAMREKLLGPDHIEVALSLQLLAAIYRQQGKFAAAEPLLNRALNIRERALGLE
                     NPDVAASLTDLALLNLAEGNVNQTIALLSKSNDVTERDLCRNLVTGSERQKLLYLVTT
                     NPKTDVTISLHAQTAPKNRDALRAGLTVVLRRKGRGLDAMAESIEELQRRAAPEDRGL
                     LSELARAKTRLSSLILRGPDTQTSEAYQAQIKDLTDQVDQIEAKVSVRSAEYRAQTQP
                     ISLESIQKAVPDDAALVEFVSYRPYDAKTDTFATRHYLAYVLTRTGDPKWADLGEAEP
                     IEAAVGDLRQTLSSAKKDLENEVKPAARKLEKLVMKPVRSLIGSRKHLLISPDGALNL
                     IPFAALMDEKGKFLVETYSLTYLTSGRDLLRLQVKIESQSPALVMADPDYDKGAGPKL
                     FGQSFSPLARLGGTHQEGSQLKALFADATLKMEGEATKQALKSAVKPALVHIATHGFF
                     LRETPQTNSVAESGTRNLTKVQSLDLNPETVHQTNPLLRSGLFFAGANRGGSQTENDS
                     TLTALEAASLNLWGTKLVTLSACDTGLGDVKVGDGVYGLRRALVLAGSESQMMSLWPV
                     SDAATRDLMVGYYQRLKAGEGRSQALRQVQLKMLKSPVRRHPYYWASFIQSGEWANLD
                     GKR"
     gene            2905..6072
                     /locus_tag="PKZ53_01490"
     CDS             2905..6072
                     /locus_tag="PKZ53_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ39133.1"
                     /translation="MWARFFILGGLTVTTFFPFSTQSHLCWVLAQREIAPAQIDTSEK
                     EITALCEQAKKLEAAGKYDEALPLVQKALAQAEQSVGSEHVLTARCLTLLATVWYDKA
                     DYTQAEECARRAISIREKQLGSEHHDLAESLNILGLVLKSKGNFPMAEPLYLRAIAIL
                     EKTVGPDHSEVAESTYNLAELYRVKGDYQRARPLVQRALTIFEKELGPEHPNVAFCVN
                     SLGRLFWLEGKFDEAEPLFLRALELRKKVLGPEHPGVALSLNNLGLLYQDKGEYLRAE
                     PYFRQSLAIHEKVFGANHPGVAASYINLGMLYSEKGDLQEAESYFEKALPIQEKVYGA
                     DHPTIANTLNILGGLILKQGDSTRAEQFFQRALTIQEQKLGSEHPLTINTLSNLAFLY
                     QDRGDLALAEPILQKVVARNLKVLGPNHPDLATDYNNLAGLYQKKGDLAQAEANYQKA
                     LAIREKILGPENPQVVTTLSNLAVLYQCKGDLDEAIALRVRSNQATEHDLRRNLGWGS
                     ERQKLLYFNTTLNRTERTIALHIQSAPQNQEALWAALTIILQRKGRILDAMAEGIETL
                     RKQAAPEDRALLDELAAAKTRLSNLILKGPGTGDPEKYRAELKQLEETIDQLEGKVSA
                     RSAEFRAQTQAISLEAVQKAIPPGAALVEYATYRETDLKTGGFGPRRYAVYVLTSQGN
                     PKWADLGDATVIDQAVAQFRKSIHLTQGTRILQVAGKPGQTKQSLIAAAQILDHLVMK
                     PVRALIGGNTHLLISPDRALNLVPFAAFVDEKGHFLVEKYLITYLTSGRDLVRLQVKL
                     ESQSPPLVVADPDYADGKGPQLFGQSFSPLPRLEGTRLEGTRLKALLPGARLKMEGEA
                     TETALKSISKPSILHIATHGYFLDDLSDANSLTDSSPRQIGLKTGSSTTSAALGSANP
                     LLRSWLFLAGANQGGNQENDGILTALEASQLNLWGTRLVVLSACESGLGEVRNNQEGM
                     YGLRRALVLAGSEAQVMSLWAVSDKATTELMVEYYQRLKAGAGRAAALRAVQLKMLKD
                     PKRRHPYFWASFIQSGEWANLDGKR"
BASE COUNT         1555 a         1639 c         1674 g         1364 t
ORIGIN      
        1 acagtgggaa ctatgccccg gctgagcctt attatttacg tgcctgcgcc attgccgaaa
       61 aaatccacga gcccgtccac gaacgcgtcg gaaattttat ttatcaactc gggttgttct
      121 atcaactccg cggcgacttt gaccgggctg aagaactctt tcagcgaact cacgccattt
      181 gggaaacaac tctcgggccc gatcactgga agattaccca gagcctgtgc attctggcgt
      241 cgctgtacct tgatacaggt gagtatgcca aagccgagcc gttgctcaac cgttcgctgg
      301 cgttagcgga aaagattggt ccggaaagta attatgtcgc ccagacgctt gatctcctgg
      361 cctcgatgta ccgggccaga ggggattatg aaaaggctga accctattac cagcgggcgt
      421 tggtgttgtg ggaaaaagca cgtggagctg acaatccaag cgtggcgctc catctggtga
      481 atctgggaga cctgtaccgg gcgatgggtg aatttgccaa agctgaaccg ctttatcagc
      541 gggccctggt attgagggaa aaggggtttg gaaaagaaca tcggttcgta gccgattgca
      601 tttttggaca ggcagaactg gcgaaagaaa aaggcgatct ggcccaggcc gaagcgctcc
      661 atgtaaaagt tctggccatg cgtgaaaagc tcctggggcc ggatcatatt gaagttgcct
      721 tgagtctcca gttgctggca gccatttatc gccagcaggg caagtttgcc gccgccgaac
      781 cattgctcaa tcgggcgctc aacattcggg aaagggcgct tggtttggaa aatccagacg
      841 tggctgccag cctgaccgat ctggccctgc tcaatctggc tgaaggcaac gttaaccaga
      901 caattgcttt actttccaaa agcaatgatg tgaccgagcg ggatttatgc cgcaatctgg
      961 tgactggctc cgagcgtcag aaactgctct atttggtaac gaccaacccc aaaacggatg
     1021 ttaccatttc gcttcacgcc cagaccgcac cgaaaaaccg cgatgctttg cgggccgggt
     1081 tgacggttgt gctgcggcgc aaaggacgcg ggctcgatgc gatggccgaa agcatcgaag
     1141 aattgcaacg ccgtgccgca ccggaagacc gggggctact gagtgaactg gcacgggcca
     1201 aaacccggct ctcaagcttg atcttgcgtg gccccgatac ccaaacaagt gaggcgtacc
     1261 aggcgcaaat caaagacctc accgatcagg ttgaccagat tgaagccaaa gtcagtgtgc
     1321 gcagcgccga atatcgcgcc cagactcagc caatttcgct tgaatcaatc caaaaagcgg
     1381 tgcccgacga tgccgcgctg gtcgagtttg tttcatatcg tccctatgat gccaaaaccg
     1441 atacttttgc aacgcgacac tatctggcat atgtgctgac cagaactggt gaccccaaat
     1501 gggcagattt gggtgaggcc gaacccattg aagctgcagt gggtgactta cgacagaccc
     1561 tgagcagtgc gaaaaaagac ctggagaacg aagtcaaacc agccgcccgg aaacttgaaa
     1621 aactggtgat gaagcccgtg cgctcgctga ttggctcgcg caaacacctg ctgatctccc
     1681 cggatggcgc actcaacctg attccttttg cggcgttgat ggatgaaaaa gggaaatttc
     1741 tggttgaaac atattcgttg acctacctga ccagcgggcg tgatttgctg cggttacagg
     1801 tcaaaattga aagccagtct ccggcactgg tgatggccga tccagattat gacaaaggcg
     1861 ctggtccaaa actctttggc cagtcatttt cgccactggc tcggcttggc ggaacacacc
     1921 aggaaggctc gcaactcaaa gccttgtttg ctgacgccac cctcaaaatg gaaggtgaag
     1981 ccaccaaaca agcgctgaaa tctgccgtca aacccgccct ggtgcacatt gccacgcacg
     2041 gatttttctt gcgagaaaca cctcagacca attctgtggc cgaaagtgga acccggaatt
     2101 taaccaaagt ccaatcgctt gatctcaacc cggaaacggt tcatcaaacc aatccactct
     2161 tgcgctccgg gctctttttt gccggcgcca atcgcggtgg cagccagact gaaaacgaca
     2221 gcaccttgac cgcacttgaa gccgccagcc tgaatttatg gggcaccaaa ctggtgacgc
     2281 tctccgcctg tgacaccggg ctgggggatg tcaaagtcgg cgatggcgtg tatgggttgc
     2341 gccgggccct ggtcctggcc gggagcgaat cacaaatgat gagtctgtgg ccggtttcag
     2401 atgcggccac ccgtgatttg atggttggtt actatcagcg cctaaaagcc ggggaaggcc
     2461 gcagccaggc actccgccag gttcaactca aaatgcttaa aagcccggtt cgccgccatc
     2521 cttattactg ggcatccttt atccaatctg gcgaatgggc gaatctggac gggaaacgct
     2581 gaatgaagaa tgaagaattg agaattgaga gtgcagaatt gagaatggcc tgaattttgc
     2641 ccaaagtcgc ctggccttgc tcaaaaatca gcgccgacca gttcgggacc tgactcgcac
     2701 ggggaatccc agtgaacctt gagcggactg aaaattcacc gaagtctctc tggccactcc
     2761 caccaaactg acttccaccc ggttctttct gtcctttctg tcctttgatt ttagctcaac
     2821 caaccacatc cgagaccaca tctgtgtctg ccactgtggc cacaattgac ttttcccaga
     2881 ttgggatgag aactggagcg tgttatgtgg gcgaggtttt tcatacttgg tgggctgacg
     2941 gtgaccacct ttttcccttt ttcaacccaa tcccatctct gctgggttct ggctcagcgt
     3001 gaaatcgctc cagcccagat tgatacctca gaaaaagaaa tcactgccct gtgtgaacag
     3061 gcaaaaaaac tggaagcagc gggcaaatat gacgaagcgc ttccgctggt ccaaaaagcc
     3121 ctggcccagg cggaacaaag cgttggttca gaacacgttc tgaccgccag atgtttaacc
     3181 ctgctggcga cggtgtggta tgacaaagcc gactatactc aagccgaaga gtgcgcccgg
     3241 cgagcgattt ccatccgaga aaaacaactt ggttctgaac accacgacct ggctgaaagt
     3301 cttaacattc tgggtttggt cctgaaatca aaagggaatt tcccgatggc ggaaccgctc
     3361 tacctgcggg cgattgccat tctggaaaag accgttggcc cagaccattc tgaggttgcg
     3421 gaaagtacct acaacctggc tgaattgtac cgggtcaagg gtgactatca gcgggctcga
     3481 ccactggttc aacgcgcctt gaccattttt gaaaaggaat taggcccgga gcatcccaac
     3541 gttgcctttt gcgtcaacag tctgggccgg ttgttctggt tggaagggaa atttgacgaa
     3601 gctgagccat tgtttttgcg cgcgcttgag cttcggaaaa aagtgctggg gccagagcat
     3661 ccaggtgtgg cgctcagtct caataacctg ggattgctct atcaggacaa aggcgaatat
     3721 ctccgggcag agccatattt tcggcaatca ctggccattc atgagaaagt atttggcgcc
     3781 aaccatcctg gcgttgccgc cagctatatc aaccttggca tgctctattc tgaaaaggga
     3841 gacctgcagg aagctgaatc atatttcgag aaagccctgc cgattcagga aaaagtgtac
     3901 ggtgcggatc atccgaccat tgccaacacg ctcaatatcc tgggtgggtt gattttgaaa
     3961 cagggagata gcactcgtgc cgaacaattt tttcagcgag ccctgacgat tcaggaacaa
     4021 aaacttgggt cggaacatcc tttaaccatc aatactttaa gcaatctcgc ttttttatat
     4081 caggatcgag gggatttagc gctggcggag ccgattttgc aaaaagtagt ggcccgaaac
     4141 ctcaaggttc ttggaccaaa tcatccggat ctggctactg actataacaa tctggcgggt
     4201 ctgtatcaga aaaagggcga tctggcacag gctgaggcga attaccaaaa agccctggcg
     4261 attcgggaaa agattctcgg tcctgaaaat ccgcaggtgg tcaccacctt gagtaatctg
     4321 gcggtactct atcagtgcaa aggtgatctg gacgaggcca ttgctttgcg tgttcgcagt
     4381 aaccaggcta ccgagcacga tttgaggcga aatctgggct ggggttcaga acgtcagaaa
     4441 ttgctctatt tcaataccac actcaaccga accgagcgga cgattgcact gcacattcag
     4501 tcagcaccac aaaaccagga ggcgctctgg gctgcgctga caataatttt gcaacgcaaa
     4561 ggacggattc tggacgcgat ggccgagggg atcgagacat tgcgcaaaca ggccgcaccg
     4621 gaggatcgag cgttgctcga tgaactggca gccgccaaaa cccggctctc aaacctgatt
     4681 ctcaaaggac ctggaacagg tgatcctgaa aaataccgcg ccgaactcaa acaactggaa
     4741 gaaaccattg atcagcttga aggcaaagtc agcgcccgca gtgctgaatt tcgtgcccag
     4801 acacaggcga tttcccttga agccgtccaa aaagccattc cacctggggc agctctggtc
     4861 gaatatgcca cgtatcgcga aactgacctg aaaacaggtg gatttggtcc ccggcgctat
     4921 gcggtgtatg tcctgaccag tcagggcaat ccgaaatggg ccgatctggg agatgccaca
     4981 gtgattgatc aggcggttgc tcagttccga aagtcaattc atctgaccca ggggacgcgc
     5041 atcctgcaag tcgctggaaa accaggccag acaaagcaaa gcctgattgc cgccgcccaa
     5101 atccttgatc acctggtgat gaaaccggtg cgggccttga ttggtggcaa tactcacctg
     5161 ctcatttcac cagacagagc actcaatctg gttccatttg ccgccttcgt tgatgaaaaa
     5221 gggcatttcc tggttgagaa atatctgatt acctatctga caagtggccg cgacctggtt
     5281 cgattgcagg tcaaacttga aagccagtcg ccgccgctgg tggtggccga tccagattat
     5341 gccgacggaa agggcccaca attgtttggc cagtcatttt ccccgcttcc ccgattggaa
     5401 ggaactcgcc tggaaggaac ccgcctcaag gcgctcctgc ctggggcacg tttgaaaatg
     5461 gagggcgagg caaccgaaac cgccctcaag tcaatttcga aaccttcgat tcttcatatt
     5521 gcaacgcatg gctatttttt agatgacctc tcagacgcca attccctcac cgacagttca
     5581 ccgcgtcaga ttgggctcaa gactggatcc tcaacaactt ctgcggcgct gggcagcgcc
     5641 aatcctttat tgcggtcctg gttgtttctg gctggtgcca atcaaggcgg aaatcaggaa
     5701 aacgatggta ttttgactgc cctcgaagcc tcgcaactca atttgtgggg cacccgactg
     5761 gtggttcttt cggcctgtga gagtgggctt ggggaagtaa gaaataatca ggaaggcatg
     5821 tatgggcttc gtcgggcact ggtactggcc gggagcgaag cccaggtgat gagtttatgg
     5881 gcggtttccg acaaagccac gacggaactc atggttgagt attaccagcg actcaaagcc
     5941 ggagctggcc gggcggcggc cctgcgagcc gtccagctca aaatgctcaa agacccaaaa
     6001 cggcggcacc cctatttttg ggcatcgttt atccaatctg gcgaatgggc caatctggac
     6061 ggcaaacgct gaatgaagaa ttgagaatga agaattgaga atgaagaatt gagaatgacc
     6121 tcaattttgc ccaaatgagc accacctggc tcacatatca gtgccggtca gttcggaatc
     6181 cggctgtcac cagggaatcc cggaccattt tgagtggcct ggaaatgtac ca
//
LOCUS       DAOQDO010000050         6211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7580_length_6211_cov_3.195582, whole genome shotgun sequence.
ACCESSION   DAOQDO010000050 DAOQDO010000000
VERSION     DAOQDO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7580_length_6211_cov_3.195582"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKZ53_01495"
     CDS             <1..538
                     /locus_tag="PKZ53_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39134.1"
                     /translation="FMDVMMPGMTGHDVCQLVRLDPALSEIPFIFLSAKRDADERLEG
                     IEAGADDYVGKPFSLQELHLRVNAALAKHKQLVATGQRISADPLVQLSLAQCIDLIVR
                     HNLSGKLSVLLKGTVQGTIFFEGGHAVHAKLEDKIGEAAVREILASQLLMYSFEAYEV
                     TDQMTMAMDISRAIKRPN"
     gene            778..1197
                     /locus_tag="PKZ53_01500"
     CDS             778..1197
                     /locus_tag="PKZ53_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNJ39135.1"
                     /translation="MQPTMSEAPEKKIRILIAKPGLDGHDRGAKYIARVLRDAGMEVI
                     YTGLRQTPEQIVTAALQEDVDAIGVSILSGAHMTLFPRILELMKDKGLDDVLLFGGGI
                     IPDEDIPKLKGLGVGEIFTPGALSEDIIRYLTESIRK"
     gene            1366..2169
                     /gene="pgeF"
                     /locus_tag="PKZ53_01505"
     CDS             1366..2169
                     /gene="pgeF"
                     /locus_tag="PKZ53_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNJ39136.1"
                     /translation="MFELRHHGTTAVIVCSKLENLGFINGFSTRLGGVSPLPEAALNL
                     GHFGGDTHENVDENRRRFLTVLNAGTYPLHTLKQVHSALVHQAHPQDVTGPKREGDAL
                     VGQEAGKLVGVLTADCLPVLIVDPTTQAYTAIHAGWRGTLQRITEQAIQTLSETFGTV
                     PADCHVALGPCATVAQYEVGDELVDQFREVFHQAADTFFQRPAGSRKAHLDVQAANLY
                     QLATAGIPRDQIYISEYCTMQRNDWFFSYRCEKETGQVGRLLSVVGRKL"
     gene            2207..4405
                     /locus_tag="PKZ53_01510"
     CDS             2207..4405
                     /locus_tag="PKZ53_01510"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNJ39137.1"
                     /translation="MKQGKKRTPSPTQPEPSIELTAGISPSDPQFTLPPSTSVIVPLL
                     TFGMALTGYWFTMARTVTLVDSGELILTAATLGVAHPPGVPLYTLLGFFFSKLPLGSM
                     AFRITVMSGVFAALTAAMVTLLTREIWWLVREKEELARRRPSSKKTEPAGALTSRTPW
                     LVVCAEILIPLGTGLAFAFSKTLWYYASVAEVYSLNLALLSLVIFLVTRWLRVKTQNL
                     VTQAEPRETWWLIAGATLVYGLALGVHHVTILLTFPAFVVLVWMSEGTAFFRSREFAW
                     SIGTVVAGLVLVYAYLPLAALREPVLSWGYPATVERFLWHISGKQYQVNLFAADGEKV
                     AAEFRTFLVLLVEQLTPAAVVGMLAGMWALWHKNRAWLMFLWLVVVLNVAYSINYEIA
                     EDKDAYYLPTHLACALMLGIGLEFLVEQARLFGKAAVAVAGVAIVALPAVCFGLHFQE
                     NNKRNYLIARDYVENTLRSVAPGGLLLTLDWQIYSPYLYLRHLEGFRTDATVVDVNLM
                     RRSWYVDRYLPRQYPEMMQACAAEKQAFLQDLSLFEHDQPYNAEQIQKHFLALIEAMV
                     KYHLSRQGVWLMLPMEPGVGQGYTWVPEGLTMHVVTESQAEAQRKYTTAPALELRGLL
                     DQSVFLDEVAKGKVAETYSMMIANRGKYLTLIGQPDAAFEQLRLALQLDSDNDRAYEF
                     MGDIYSSQGLLGAAQDAYQTALQFNPNNTSARQSLQRLTPPPASATPPKP"
     gene            4412..5260
                     /locus_tag="PKZ53_01515"
     CDS             4412..5260
                     /locus_tag="PKZ53_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ39138.1"
                     /translation="MMNFRSRLLSLLLFISLVTAPVAGISVLAAGQKAVQQPVRRVEL
                     EPEKTKSTILTADETVAEDPEFKKFVEKYGASINAKLSEVIAHTPREIRRGAGSDLGR
                     IVADAMLLVAHQKTKRKIDLAISNTYGMRGDLPAGDITIANVFTIMPFENQLVLLELT
                     GQQLLEGFEYVATHYDESAGFPLANCIVRVKDEKLVSVEIGGVPLDPKKTYTLATSDY
                     LYYGGDGYHFSQGKNFRHVGISLRDAIIIYMRQQKNVEIPTQARFINESAPTDPHRET
                     PAGGQR"
     gene            5257..6174
                     /locus_tag="PKZ53_01520"
     CDS             5257..6174
                     /locus_tag="PKZ53_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ39139.1"
                     /translation="MKRRTFLTTSLAATTGVALGGFPAILKAQSAETRITVLHTNDTH
                     SQIEPMVAGVNVGKGGISRRMTLVKQVRQESPNTILVDGGDVCQGTPYFNFYRGEVEY
                     KAMSMMGYTAGTLGNHEFDNGVGPLTKALSFANFPIVNANYDFSGTPKLRARVKPFIV
                     RTFGGIRVGIFGLGVNFKNLVLPDNHKGITYRDPIEVSRRVVKQLREREGCDFVLALS
                     HLGYPKDNIGTNLLMHDIMVAEQVDGIDFIVGGHTHMWMDEPDVITRPGGGRTLLFQA
                     GYAGVRIGRVDFFFRDRKLAAWNGKPNEE"
BASE COUNT         1449 a         1554 c         1666 g         1542 t
ORIGIN      
        1 cttcatggat gtcatgatgc cagggatgac cgggcacgac gtttgccagt tggtgagact
       61 cgatccggca ctcagtgaaa ttccatttat ttttttgagt gccaagcgcg atgcggatga
      121 acgtctggaa ggtatcgaag ctggtgcgga tgactatgtc ggcaaaccct tttcgttgca
      181 ggaactccat ttgcgtgtga atgcggctct ggccaagcac aaacaactgg ttgccaccgg
      241 acagcgcatt tcagccgacc cgctggttca actgtcactg gcccagtgta ttgacctgat
      301 tgtccggcat aacttgagcg gcaagttgtc ggtactcctc aagggaacag tccagggcac
      361 gatctttttt gaaggtgggc atgccgtcca cgccaagctg gaggacaaaa tcggagaggc
      421 ggcagtgcgt gaaattctcg cctcgcagct tctgatgtat tcctttgaag cctatgaagt
      481 gaccgatcag atgacgatgg ccatggatat ttcacgggca atcaaacgac cgaattaaag
      541 gattccgggt tccgggttcc gggttccggg ttctgggttt tcgaacccaa gctcacacgt
      601 cctgtgttat tccaaatcct gattgtatgt tgaacccact attgcccggt ttccaacggt
      661 tcaatgattg agcagcttgt atttccctga accctgaacc ctgaacccta aattgcgttg
      721 ccccacgatt caaaaactgc cacccgtgca ggaaattcaa attttgttga aagaattatg
      781 caaccgacca tgagtgaagc acctgaaaag aaaattcgaa tccttattgc caaacctggc
      841 ctggatggtc acgaccgggg ggcaaaatat attgcccgtg tattgcggga tgcgggtatg
      901 gaagtcattt acaccgggct gcggcaaact cccgaacaaa ttgtcacggc tgccttgcag
      961 gaagacgttg atgcgattgg cgtttcaatt ttatcagggg cgcatatgac cctgttccct
     1021 cgcattttgg aactgatgaa ggataaaggg ctggacgatg tgctgctttt tggcggcgga
     1081 atcattccag atgaagatat tccaaaatta aaaggtcttg gcgttggtga aattttcacc
     1141 cctggcgctc tttccgaaga cattatccgc tatctgaccg aatctatccg caagtaatcc
     1201 ttgggctgaa aaaaccaggg ctgaagaaaa cgggctgaaa acatcgggct gaagaaaatg
     1261 ggctgaagaa gttgggaaat tcagtccgga gccccaagtc ttcagccccc agccctgagc
     1321 ccaacttctt cagccctgag cttgcgaatc ttcagcccga ctcttatgtt tgagcttcga
     1381 catcacggta ccactgccgt cattgtgtgt tccaaattgg aaaacctggg gtttatcaac
     1441 ggattcagca cccgtctggg cggcgtgagc ccacttccgg aagccgcact caatttggga
     1501 cattttggcg gcgacaccca cgaaaatgtg gatgaaaacc ggcgacgatt tttgacggtt
     1561 ttgaacgccg gcacctatcc acttcatacc ttaaaacagg ttcattctgc gctggtccat
     1621 caggctcatc cccaggatgt caccgggccg aaacgggaag gcgatgcgct ggttgggcag
     1681 gaagccggaa agctggttgg tgttttgacc gccgattgtc tgccggtgct gattgtggat
     1741 ccaacaaccc aggcctatac cgcaatccat gccgggtggc gagggacgct ccagcgcatc
     1801 accgaacagg cgatccagac actttctgaa acctttggca ctgtgcctgc cgactgtcat
     1861 gtggcgcttg gaccctgtgc aacggttgcc cagtatgaag ttggtgatga actggttgat
     1921 cagtttcggg aggtttttca ccaggcggct gacacttttt ttcagcgccc ggcaggcagc
     1981 cgcaaggccc atctggatgt acaggccgcc aacctttatc aactggcgac ggcaggaatt
     2041 cctcgtgatc aaatctatat ctccgaatac tgcaccatgc agcgcaatga ctggtttttt
     2101 tcctatcggt gcgaaaaaga aactggtcag gttgggcggc ttctgtcagt tgtcggacgc
     2161 aaactctgaa gccattgcat aattcataat tgacacgccg ttcacgatga aacaaggtaa
     2221 aaaacgaact ccatcaccaa cccaacctga gccgagcatc gagttaaccg ctggtatatc
     2281 tccttcagac ccacaattta cgctcccgcc ctcaaccagt gtgatcgtgc cattactgac
     2341 ttttggaatg gcattgaccg ggtactggtt cacgatggct cgaaccgtca cgctggttga
     2401 cagcggcgaa cttatcttga cggcagccac cctgggcgtt gcgcacccac caggagttcc
     2461 gctctatacc ttgctgggat ttttcttcag caaacttccc cttggcagca tggcctttcg
     2521 catcactgtg atgtcaggtg tgtttgcggc gctgacggcg gcgatggtga cactcctgac
     2581 ccgagaaatc tggtggctgg tgcgtgaaaa agaagaactg gccagacgtc gcccgagcag
     2641 caaaaaaacc gaaccggctg gcgccttgac ttcgcgaacg ccgtggctgg tggtgtgtgc
     2701 cgagattttg attccactcg gtaccgggct tgcctttgct ttctccaaaa cgctctggta
     2761 ctacgcctcg gtggcggagg tgtattccct gaacctggca ctgttgagcc tcgtgatttt
     2821 tctggtgacg cgctggctgc gggtcaaaac tcaaaatctg gtgactcagg ctgaaccacg
     2881 cgaaacatgg tggctgattg ccggggcaac gctggtttat ggcctggccc tgggtgttca
     2941 ccacgtgacg attttgctta cattcccagc ctttgtagtt ctggtctgga tgtcagaagg
     3001 aacggcgttt tttcgcagtc gtgaatttgc ctggtcaatt gggacggttg tggctggatt
     3061 ggttctggtc tatgcctatc tgccactggc tgccctgcgg gaaccggtgt tgagttgggg
     3121 ctacccggcg acggttgaac ggtttctctg gcacatttcc ggtaaacagt atcaggtcaa
     3181 cctgtttgct gcagatggtg aaaaggtcgc ggctgaattc cgcacgtttc tggtgcttct
     3241 ggttgaacaa ctgaccccgg ctgctgtggt gggaatgctt gccggaatgt gggcactctg
     3301 gcacaaaaac cgcgcctggc ttatgtttct ctggctggtg gtggttctga acgtcgctta
     3361 ttcaatcaat tatgaaattg ccgaggataa agacgcttac tatctgccga ctcatcttgc
     3421 ctgcgccctg atgcttggga ttgggcttga gttcctggtg gaacaggcgc gactttttgg
     3481 gaaagctgcc gttgctgtgg ctggtgtggc gattgtggcg ttgccagccg tctgttttgg
     3541 acttcacttt caggaaaaca acaagcgcaa ctatttgatt gcgcgtgatt atgttgaaaa
     3601 caccctgcgc agcgtggcac ccggaggatt gttgctgacg ctcgattggc aaatatattc
     3661 gccctatttg tatctgcgtc atctggaagg gtttcgcact gatgcaaccg tggtggatgt
     3721 caatctgatg cgacgctcct ggtatgttga tcggtattta ccgcgacaat atcccgagat
     3781 gatgcaggcc tgtgcggctg aaaaacaggc gtttctgcaa gatttatcgc tctttgaaca
     3841 tgatcagccc tacaacgccg agcagattca aaaacacttt ctggccctca tcgaagccat
     3901 ggtgaaatat cacctctcgc ggcaaggggt gtggttgatg ttgccaatgg aaccaggtgt
     3961 tggtcaggga tatacctggg tgcctgaagg tctgaccatg catgtggtta ctgagtcaca
     4021 ggccgaagcc cagcgcaaat atacaaccgc accggccctg gaattacgtg ggttgctcga
     4081 tcaatccgtg tttttggacg aagtcgccaa aggaaaagtc gctgagacct actcgatgat
     4141 gattgccaat cgcggaaaat atttgacttt gattggccaa ccagacgctg catttgagca
     4201 gcttcgactt gcacttcaac tcgattcgga caatgatcga gcctacgaat ttatgggcga
     4261 tatctatagt tcgcaaggtc tacttggagc cgcccaggat gcctatcaga ccgccctgca
     4321 gttcaatccc aacaacacca gtgctcgtca gagtctgcag cgtttaaccc cgccaccagc
     4381 atcggcgacg cctcccaaac cttaaacacg catgatgaat tttcggtcgc gtttgctttc
     4441 cttgttgttg ttcatttcac tggtcaccgc accagtggca ggcatttcgg tcctggccgc
     4501 cggccaaaag gctgtccagc agccagttcg ccgcgtcgaa cttgagccag aaaaaaccaa
     4561 atccacgatt ctcacggctg atgaaacagt ggctgaagac ccggaattta agaaatttgt
     4621 tgagaaatat ggggcttcaa tcaatgcaaa gctttcagaa gtaattgccc ataccccgcg
     4681 tgagattcgt cgtggggctg gttctgattt ggggcggatt gtggccgacg caatgttgct
     4741 ggttgcccat cagaaaacca aacgaaaaat tgatttggca atctcaaaca cctatggcat
     4801 gcggggcgat ttaccggctg gcgacatcac cattgccaat gtctttacca ttatgccgtt
     4861 tgaaaaccag cttgtgttac tcgaattgac cgggcaacag ttgctggaag gctttgaata
     4921 tgtggcgact cactatgatg aatcagccgg ctttccgctg gcgaactgta tcgtgcgagt
     4981 caaagacgaa aaactggtga gtgttgaaat tggtggcgtg ccacttgatc caaagaaaac
     5041 ctacacgctg gccaccagtg actatttgta ctatggcggt gacggctatc actttagcca
     5101 ggggaaaaat ttccgacacg tcgggatttc gcttcgagat gcgatcatta tctatatgcg
     5161 ccagcaaaag aacgtcgaga tcccaacgca ggcccgattt atcaatgaat cggcgccaac
     5221 tgaccctcat cgggaaacac cagcaggagg ccagcgatga agcgacgaac tttcctgacg
     5281 acaagtctgg cggccacaac tggcgttgca ctgggcggtt ttccagccat tctcaaagct
     5341 cagtccgccg aaacgcggat cacggtttta cacaccaatg atactcattc ccaaattgag
     5401 ccgatggtgg ctggggtaaa tgttggaaaa ggaggcattt cccgtcggat gacgctcgtc
     5461 aaacaagtcc gacaggaatc tcccaatacc atcctggttg acggcggcga tgtttgccag
     5521 ggaacaccct atttcaattt ctatcggggt gaagtcgagt acaaagccat gtccatgatg
     5581 gggtacacgg caggcacgct tggaaatcat gagtttgaca atggcgtggg cccgctgacc
     5641 aaagccctat cctttgccaa ttttccgatt gtgaatgcca attatgattt ttcaggaacc
     5701 ccaaaattac gagcacgggt caaacccttt attgtgcgga ccttcggggg catccgggtc
     5761 gggatttttg ggcttggtgt caatttcaaa aatctggtct tgcccgataa ccacaaaggg
     5821 ataacgtatc gtgatccgat tgaagtctcg cgacgggttg tcaaacagtt acgtgaacgt
     5881 gaaggatgtg actttgtcct ggcactctct catttaggct atcccaaaga caatattggc
     5941 accaatctgc tcatgcacga cattatggtg gctgaacagg ttgacggaat tgattttatt
     6001 gtcggcggcc acacccatat gtggatggac gaaccagatg tgattactcg tccagggggc
     6061 ggacgaaccc tgttgttcca ggccgggtat gcgggggtca ggatcgggcg cgttgatttc
     6121 tttttccgtg atcggaaact ggctgcctgg aacgggaaac cgaatgaaga atgaagaatg
     6181 aagaatgaag aatgaagaat gaagaatgaa g
//
LOCUS       DAOQDO010000051         6202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7600_length_6202_cov_3.481373, whole genome shotgun sequence.
ACCESSION   DAOQDO010000051 DAOQDO010000000
VERSION     DAOQDO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7600_length_6202_cov_3.481373"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKZ53_01525"
     CDS             complement(<1..770)
                     /locus_tag="PKZ53_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ39140.1"
                     /translation="MRRISWLWIVVVLVIGLFGPLGLAQETPQTNEETDEGVRSAFLV
                     TRKKPVSPPVTKGKRPPKIGFPLSVPRLGLGISLYARLRGNPTGMAVRVDPSQIFQAG
                     DALRFVLEPNTNGFVYVFYRENDGEPVMLYPDVRLSRGLNQILAHVPMELPSSRELNP
                     QLQWFYFGNKPAIERLYFVVARAPLPGIPIGNELLAYCQQFPAACPWKPPLAAWQQLE
                     GYVSSIPKQMSKLTRLGQSLTAGEGLAIQRDFGLKPADP"
     gene            complement(819..2555)
                     /locus_tag="PKZ53_01530"
     CDS             complement(819..2555)
                     /locus_tag="PKZ53_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39141.1"
                     /translation="MLALQSLLQNRYRIESLLGKGGMGAVYRATDTRLSNVVALKETL
                     FQDERLRRAFEREAKLLAGLFHPALPKVSDYFSEGDGVFLVMAYVPGEDLQQMAERQP
                     GPFPLSQVLDWADQILDVLEYLHGQQPPIIHRDIKPANLKLTPDGRIILLDFGMARGG
                     GGSSIAGYSVNYAPPEQIDQISTDARSDLYALGATLWHLLSGESPPNSVTRAMTVANG
                     EPDPMLPVHRLNPGVPQEISGILHQAMALDRERRPASARVMRQMFREAAQPVLAKQSA
                     KTLIPGGLGPTQLPGTRMALKVEAPPPLAATQMAETIESPVFHPQPAPVGYQSPPVSD
                     FQPVQSEGPPPISGSFQPPAGPAVHHRANHSLLWAGVVAVLAIAGSLLAYRYIAGNSE
                     GGGGVTGNIRGEVVALTLEVDRGGKTRKTTDEVTFKADEVFKFKLTPVRDGYLYLVAP
                     GEDKTQTTFLTSQPATDTGLTSNRLLANQEFEFPAGKEHWIGLRDDSPTTRFTIILSP
                     TQLKEPKFFARESGTALTAGEVKTFDEFCQENRVQLKRTVDSESVIRLALPESREAGK
                     PIVFEIEVKREK"
     gene            complement(2713..4017)
                     /locus_tag="PKZ53_01535"
     CDS             complement(2713..4017)
                     /locus_tag="PKZ53_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ39142.1"
                     /translation="MRNHTRLLIAIVSVLLVVVPFVVASVRPTELQELPFLVMFPELN
                     TTQFVPPQIPIPHSRLTRLEVLIPKPLSDQMTYGGIFLSINGQAANRILDKVNTPAGK
                     LMKIDLRRLPGFLLRDGKNSIEVLAELADGREVYNACVISTPYGLPGVGVPKSGSPVE
                     QFTGQKYALVIGISRYLYGNSLKFADADARAFRDFLLSRQGGSFRPENILLIENESAT
                     LGGIQQALETVVKQVTANDLLVFFLAGHGGPDPGSRKTRYFMTYDTGQDFTRSALPMT
                     LLRDRLTKDLRAKRLVCFIDTCHSAGLAGDPQAGGFGMRDFGLNLINQDFESLLYGAQ
                     GIAVLTSSDIDEKSYESQVWDGHGVFTYFLLKGLKGAADVDRDNLVTAGEIFEYVRGE
                     VPKAVESHIQLLRQKGITAELTGQHPRAAVGNNDGLQLAIVK"
     gene            complement(4314..6041)
                     /locus_tag="PKZ53_01540"
     CDS             complement(4314..6041)
                     /locus_tag="PKZ53_01540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ39143.1"
                     /translation="MGHTHLPHRFEEPGYKETLLLRGIIELLILLLAAGLIVLSLEIK
                     ATSAPYPQDDKTKKEQKKLREYSKDIKEVLELKDYGVKKLQSKEDADMDFKSDVVRAY
                     NDMRRTHQLQAFRVNTRAPLRVMGDLEGEGGNFAARSSGAEASSGGMDALYDNPMLQD
                     YVSRIGHRLVPQNSRKLYAFKILQDPLPRADSLSTGTIYLSTGLLGMLDNEAQLAYIL
                     SHEIAHVEMDHWFYQSMLPIALAEKNEDKEKKRQRIGALMALGGMALGAKSDGLQGAY
                     QGASLGYGLGHLIGDLAIRDVKITEWNIADEDDADRFAFQNCLANGFDVQEVPKLFLN
                     LYNAVLADSRVGLGFMSSLQHVYERNEGVKLLLKENENTIRQLSSQGKLIGSHSEFEV
                     LMAELKRDNGVVAFYYDMFDMTRKNLEWSLRIRSDDAKAHYYLGRVLKLTSRTPEEKE
                     RARREFATALKLDEARGSMPGIRLQQALSLLDTSDPTKFPEAVTYIKEYVRLYKLNNG
                     GSVPPNMDILYDYMSQAGETAWFVPPTSNVTAQEVFPVVMKSSPVTTPPTNQPPPNTQ
                     VQKNPGKKN"
BASE COUNT         1421 a         1734 c         1508 g         1539 t
ORIGIN      
        1 gggtcggctg gtttcaaacc aaaatcccgt tgaattgcca gaccttcgcc agccgtcagt
       61 gactggccca ggcgggttaa tttgctcatc tgtttgggga tactgcttac atagccttcg
      121 agttgctgcc aggcagccag cggaggcttc cacggacagg cggctggaaa ttgctgacaa
      181 taggccagta attcatttcc aatcgggatt cccggaagcg gagcgcgtgc cacgacaaaa
      241 tacagtcgtt caatggctgg tttattgcca aaataaaacc actgcagttg ggggttcagc
      301 tcgcgactcg aaggcaactc cattggaaca tgcgccagaa tctggttcag tccgcgactg
      361 agtcgaacat ccgggtacag catcaccggt tcgccatcat tttcgcgata aaaaacatac
      421 acaaacccat tggtattggg ctccaggaca aaccgtaacg cgtcacccgc ctggaaaatc
      481 tgggatggat caacccggac ggccattcca gttggatttc cccgcaatct ggcatagagc
      541 gagattccaa gtcccaggcg cggcacagaa agcggaaatc caatttttgg tggacgcttc
      601 cctttggtga cgggcggtga caccggtttt ttccgggtta ctaaaaacgc acttcgcacg
      661 ccctcatccg tctcctcatt ggtttgaggc gtctcctgag ccagccccag gggaccaaac
      721 agtccaatca cgagtaccac aactatccac aaccaggata tgcgccgcat aaatgcctcc
      781 tctggagtgc ggcggcttga cgccgctttg accggtccct acttttcccg ctttacctca
      841 atctcaaaca caatcggttt tccagcctcc ctcgattccg gcaacgccag ccgaatcacc
      901 gactcactgt caacagttcg cttgagttgc acccgatttt cctgacaaaa ctcgtcaaaa
      961 gttttgactt ctcccgcagt cagtgcggtt cccgattccc tggcgaaaaa ctttggctct
     1021 ttcaactggg ttggcgaaag aattatcgtg aaccgggttg tcgggctgtc atcccggagc
     1081 ccaatccagt gctcttttcc ggctggaaat tcaaattcct gattcgccag caaacgattg
     1141 ctggtcaatc ccgtatcagt tgccggttgt gacgtcaaaa acgttgtttg tgttttatct
     1201 tcaccaggtg caaccagata gagatatcca tcacgaaccg gagtcaattt gaacttaaat
     1261 acttcgtcgg ctttgaaagt tacttcatcg gttgtttttc gagtttttcc gccccgatca
     1321 acttccaggg tcagggcaac gacttcacct cgaatattcc cggttacccc gccacctccc
     1381 tcagaatttc ccgcaatgta ccgataggca agcaacgaac cagcaattgc cagtacagcc
     1441 accactccgg cccacaacaa actgtgattt gcccgatgat gaacagcggg tccggctggt
     1501 ggttgaaagg atccactgat cggtggtgga ccttccgact ggactggctg gaaatctgaa
     1561 actggcggcg attgataccc aactggcgct ggttgaggat gaaataccgg tgattcaatg
     1621 gtttcggcca tttgagtggc agccagtggc ggcggggctt caactttgag cgccattcgg
     1681 gttccgggta gctgcgtagg ccctaaccca ccaggaatca atgttttcgc tgattgtttg
     1741 gcaagcacgg gctgcgcggc ttcgcgaaac atctgccgca tcactctggc tgaagccggg
     1801 cggcgctccc gatccagagc catcgcctgg tgcaagattc ccgaaatctc ctgtggaacc
     1861 cctgggttga gccggtgaac tggaagcatt ggatctggct cgccattggc tacggtcatt
     1921 gcccgcgtaa ctgaattggg cggagactcc cctgaaagca aatgccagag cgtcgcccca
     1981 agtgcataga ggtcgcttcg ggcatcggtg ctgatctgat caatttgttc tggcggcgca
     2041 taattgacgg aatatccagc aatgctgctg ccgccaccgc ctcgggccat tccaaagtca
     2101 agcaggataa tccgaccgtc aggggtcaac tttaaattgg cgggcttgat atcgcggtga
     2161 ataatcggtg gttgctggcc gtgcagatat tcgaggacat ccagaatctg atctgcccag
     2221 tcaagcacct gactgagcgg aaatggtcct ggctggcgtt cggccatctg ctgcaaatct
     2281 tcaccaggaa cataggccat caccagaaaa acgccgtcgc cttcgctgaa atagtcactc
     2341 actttcggga gcgccgggtg aaacagcccc gccagcagtt tggcttcccg ttcaaatgcc
     2401 cgtcgcagac gctcgtcctg aaacagggtc tctttgagtg ccaccacgtt gctgagccgg
     2461 gtatcagtcg cccgatacac ggcgcccatg ccacctttcc caagcaacga ttcaatccga
     2521 taccgatttt gcagaagtga ttggagtgca agcatggtgg aacctccttt tagggcctgg
     2581 ggctgagggc ttggggctga gggcctgggg attcactttt ggtccttttt gtccctttgg
     2641 tcctttttgt ccttttcaac gccgcaaaag ttttcatcga atcagattcg gaaaccccga
     2701 aaccctgagc ccctacttca caattgccag ttgcagcccg tcattatttc cgacagcggc
     2761 ccgtggatgc tgcccggtca gctcagccgt gatgcctttt tgtcggagca actgaatgtg
     2821 actttcaacc gcttttggaa cttcaccccg gacgtactca aaaatttcac cagcggtgac
     2881 caggttgtcc cgatccacat cagccgctcc tttgagcccc ttcaacagaa aataagtgaa
     2941 gacaccgtgt ccgtcccaca cctggctttc atatgacttt tcgtcaatgt cgcttgaggt
     3001 caacacggca attccctgcg caccatagag caggctttca aaatcctggt tgatcaaatt
     3061 caacccaaaa tcccgcatac caaagccacc tgcctgtggg tcgccagcca gaccggcgct
     3121 gtgacaggta tcaatgaaac aaaccagccg ttttgcacgc agatccttgg tcaagcggtc
     3181 acggagcaac gtcatcggga gcgccgaccg ggtaaaatcc tgcccggtat cataggtcat
     3241 aaagtaacgg gttttccggc ttccagggtc tgggccaccg tgtccagcta gaaaaaacac
     3301 cagtaaatcg tttgcagtca cctgcttgac cacggtttca agcgcttgct ggatgccacc
     3361 taatgtagct gattcatttt caatcaacag aatattttcc ggtcgaaaac tccctccttg
     3421 ccgactcagc aggaagtcac gaaacgcccg cgcatcggca tcggcaaatt tgagcgagtt
     3481 cccatagaga tagcgcgaaa tcccgatgac cagcgcatat ttttgaccgg taaactgttc
     3541 aacgggactg cctgattttg gaactcccac gccgggcaga ccatagggcg tcgaaatgac
     3601 acaggcgtta tacacttcgc gcccgtcggc cagttcggcc agtacttcga ttgaattttt
     3661 cccgtcacgc aacaaaaacc ctggcaatcg ccgcaagtca atcttcatca gcttgcccgc
     3721 tggcgtgttg accttatcca gaatccgatt cgctgcctgc ccgttgattg agagaaaaat
     3781 gccgccatag gtcatctggt cagacaacgg ttttggaatc agcacttcaa gtcgggtcaa
     3841 ccggctatgc ggaatcggga tttggggtgg cacaaactgg gtggtattca attcgggaaa
     3901 catcaccaga aacggcagtt cttgaagttc cgttggtcgt accgaagcca ccacgaatgg
     3961 aacaaccacc agcaacacac tcacaatggc aatcaacagt ctggtgtggt ttcgcatgac
     4021 cacctcctca tggttggggc tgaagattcg caagctcagg gctgaagaag gcgggctgaa
     4081 gattcgcaag ctcagggctg aagaaggcgg gtttaatacc ctaagtctga tgtcttcagc
     4141 ccaatgtctt cagcccaatg tcttcagccc gatgttttca gcccgatgcc ttcagcccga
     4201 tgttttcagc ccgatgcctt cagcccgatg ccttcagccc gatgtcttca gcccgatgtc
     4261 ttcagcccaa tgccttcagc ccgatgcctt cagcccgatg tcttcagccc cattcagttc
     4321 ttcttccctg ggttcttttg aacctgtgtg tttggcggcg gctggttagt cggtggagtc
     4381 gtcaccgggc tggatttcat caccacgggg aaaacctcct gagccgtgac attactggtt
     4441 ggcggcacaa accaggccgt ttctccggct tgactcatgt agtcatacaa aatgtccata
     4501 tttggtggga cactgccgcc attgttgagc ttatagagcc ggacatattc tttgatgtag
     4561 gtaacggctt ccggaaactt cgtcgggtca ctggtatcga gcaaactcaa cgcctgttgc
     4621 agccgaatcc caggcattga cccacgggct tcgtccagtt tgagtgccgt ggcaaattcg
     4681 cgtcgcgcac gttctttttc ttcaggcgtg cggctggtta atttcagcac ccgtcccaga
     4741 taatagtgcg ctttggcatc atctgaccgg attcgcaacg accactcaag gtttttgcga
     4801 gtcatatcaa acatgtcgta gtagaaagcc accacaccgt tatctcgttt taattcagcc
     4861 attaacactt caaattccga atggctgccg atcaattttc cctggctgga aagctgtcgg
     4921 atcgtatttt cgttttcctt gagcagcagt ttgacgcctt cattgcgttc atagacgtgt
     4981 tggagcgacg acataaaccc aagcccaact cgcgagtcag ccagcacggc gttgtataaa
     5041 ttgaggaaga gttttgggac ttcctgcaca tcaaagccat tggccagaca gttttggaag
     5101 gcaaaccggt cagcgtcgtc ttcgtcagca atgttccatt ccgtgatttt tacatcccga
     5161 atggccagat ctccgatcaa atggcctaac ccatagccca gtgaagctcc ctgataggcc
     5221 ccttgcagac catctgattt cgccccgagc gccattccac ccagcgccat caaggcccca
     5281 attcgctgcc gcttcttttc cttatcttca tttttttccg ccagggcaat cggcagcatg
     5341 gattgataga accagtgatc catttcaacg tgggcaattt cgtggctcaa gatgtaggcc
     5401 agttgggctt cgttatcaag catcccgagc aatccggttg aaagataaat cgtcccggtt
     5461 gaaagcgagt ccgctcgtgg gagcggatct tgtagaatct taaaggcata gagctttctt
     5521 gaattttgtg gcactaaccg atggccaatc cggctgacat agtcttgcag catcggatta
     5581 tcatataagg catccattcc acccgatgaa gcctcagcac cactggagcg ggcggcaaag
     5641 ttgcctccct caccttccag gtcgcccatc acgcgaagtg gggcacgggt attgacccga
     5701 aacgcctgga gctgatgggt acgccgcatg tcgttatagg cccgcaccac atcagattta
     5761 aaatccatgt ccgcatcctc tttgctctgg agttttttga caccatagtc cttcaactcc
     5821 agaacttctt tgatgtcttt gctgtattcg cggagtttct tttgctcctt tttggttttg
     5881 tcatcttgtg gatacggcgc tgaagttgcc ttgatctcca atgacagcac gatcaaacca
     5941 gcggccagga gcagaatcaa caactcaata atgccacgca acaacagcgt ttctttgtat
     6001 ccaggttctt caaagcgatg aggaagatgt gtgtgtccca taacgagccc tcctttcccc
     6061 ctcattgaga atgaagaatt tagaatttag aatttagaaa tcaaacggct gaaacctttt
     6121 agaacctgga atatagaatg tagaaattat tcttcattct tcattcttca ttcttcattc
     6181 ttcattcttc attcttcatt ct
//
LOCUS       DAOQDO010000052         6178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7653_length_6178_cov_3.194023, whole genome shotgun sequence.
ACCESSION   DAOQDO010000052 DAOQDO010000000
VERSION     DAOQDO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7653_length_6178_cov_3.194023"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKZ53_01545"
     CDS             <1..273
                     /locus_tag="PKZ53_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39144.1"
                     /translation="PLMWLTYEELVANKPQTVRRVLNFHNLDMDPEIIGRTIAHTETE
                     TRKNRFNRGIVGRGESLLSDRQKETIRRMARFYPSIDFSRLGISKE"
     gene            357..2909
                     /locus_tag="PKZ53_01550"
     CDS             357..2909
                     /locus_tag="PKZ53_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNJ39145.1"
                     /translation="MKTGYHQTGLPGPQPGNRPESKFNQRGKLLQKVFLLTLIMVFLG
                     VGMTPALRSVSAEKNGASKPRQNQQEAPPGMVLRYKPGMEPIPGQVEWDPAPATEPEP
                     TRRPDGSFTTLVGTDDCPGTRICSLPFNDSDTTVGANNTINTLPSGCNTYTQVAGPDK
                     IYTLTVGATNSITVSVDPTGANWDVSVYLVRTCPAGTGNTITSGNCVTGDDNGGSNTT
                     EGFTVTNIAAGTYFLVVDSFYPATGTSPLRQGTYDLSVTGSGLVSGDLSITKTDGITT
                     TTAGSTLTYTIVASNAGGSCTATGATIADTFSAQFTSVSWTSVAAGGATGNDASGTGN
                     INDTGVNLPAGASVTYTVTATLSGSATGSVSNTATITAPGGFSDSNSGNNSATDTDTI
                     MAAGCMVTCPSNVITNNSAGQCGAVVTFGNPTTSGSCGIVSVVPPSGSFFTVGSTTVM
                     VTTMAGPSCSFTVTVNDTEPPMVTCPANIVTGATSTAGAVVSFMPTVWDNCSGVSFVS
                     TPPSGSTFPIGTTTVMVTATDASNNTASCSFTVTVQGVELDRVYVADTANNRCQVFDG
                     AVWQLVPGTAGTVGSSSNQFRSPEAITANADGSRIYVADTGNNRIQFTTDSGATWQTF
                     ATLGSASNQVRAPQGLALDLNGNLYVADTGNNRLMRFDGGLPGPAVIFATAGSTLGKV
                     STPRGLAVDASRTLYVADFGNNRIQKFTTADVTPTPGLVATTGSSQNPGQVRSPEGVG
                     VDDEGNLYVADTGNNRVIEFPGGAAGPAVLLLNIGSTFGKARLPEGVTVCQGVVLGGM
                     ATESALIVGDTANNRIQGSLTPADPLSYELVGNPLGGVGSLIGNFRSPSKIR"
     gene            3307..4197
                     /locus_tag="PKZ53_01555"
     CDS             3307..4197
                     /locus_tag="PKZ53_01555"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNJ39146.1"
                     /translation="MKKAQLKPVLLIAILCFLMGGISLTGFAQRSGDGGGRHKRGLAG
                     QLTAIAGSQLTLTARDDKSVTVLVDANTQYRRNDAAATLADLQVNDYLSVKGKPNDQG
                     QVLAVAINASTEPPQGGGGHEGRRGPRGDFVSANAEAGTLTVKVKDGTEVTVYTTAET
                     IILRNRVQATLSDFQAGDRVSAAGRKNESGQFVAVRIMGGDAKEASRAAAQNNGVFFG
                     EVTQIASNSITFINQDGVEQTLLVSEKAVVTLKHRRVGLADLREGMHINVIGITNDQG
                     QVVATGIFGNSHRGHGRLQK"
     gene            4339..4953
                     /locus_tag="PKZ53_01560"
     CDS             4339..4953
                     /locus_tag="PKZ53_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ39147.1"
                     /translation="MGIFWRRSEPIETDEALLCQMMAGDEQAFARLYERRHQDVYRFA
                     LRMSGSEAIAEDVAQEVFLTLIQSGGKFDPSRGTVQGYLLGIARYRVLHRLAQDRTYV
                     SMETPTDMSEGVPEEYRVNHHNPLADLTHSEMIEQIQQAVLGLPPHYREVVVLCNMEE
                     LSYEAAADILDCPVGTVRSRLNRARSLLQQKLSSLAVQSSSFGS"
     gene            4989..5774
                     /locus_tag="PKZ53_01565"
     CDS             4989..5774
                     /locus_tag="PKZ53_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39148.1"
                     /translation="MKCYEFEAVVLDIVSGRLMEVRLKQAALAHAETCPTCGLRLEQE
                     QQLTAGLRAVAAQTGVAKASPKLNRELQAAFRNQFPNQIQKPQPRQLWKKHEWLALAA
                     GLVLMLAGTGLIWVLVQPGPASQIAVSATPDSLSPSPPTMPAEVKPSASTSQPSVVKP
                     QAIPSPIFKPQRPRQSPRSKPIKPQSRVETVDDFSNEFIPLTYVAASTAMDSGQIVQM
                     YISRSTLISMGLSTNVENAADYVKAQVIMSDDGVPRSIRILPE"
     gene            5836..>6178
                     /locus_tag="PKZ53_01570"
     CDS             5836..>6178
                     /locus_tag="PKZ53_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39149.1"
                     /translation="MAVLVIAMLAASSQILGQGRPGQDGGGRRPHPPFGGGPGRGDRD
                     RPGRDRESDHQDRTRTGRGTGRYFAEMRFDGKVVTGAPYSAQIVTRNTQTLRDGTKLT
                     NESTARVFRDAQ"
BASE COUNT         1524 a         1583 c         1695 g         1376 t
ORIGIN      
        1 ccgctgatgt ggctgaccta tgaagagttg gtcgccaata aaccccaaac cgtgcgccgg
       61 gtgctcaatt ttcacaatct ggacatggat cccgaaatca ttgggcgaac gattgcccac
      121 actgagaccg aaacccgaaa aaaccggttc aatcgtggga ttgtcggacg cggtgaatcg
      181 ctgctctctg atcgccagaa agagacgatt cgtcggatgg cccggtttta cccatcaatt
      241 gatttctcac gtctgggaat ttcaaaagag taaccccgca gcttgataaa ccattacaca
      301 cgcatttgat gcagtcaaaa tagttcacgt cgaataacct gggaggataa gtcgctatga
      361 aaacaggata ccaccagact ggtttgcccg gcccgcagcc tggaaaccga cctgaaagta
      421 aattcaacca gcggggaaaa ctgctccaaa aagttttcct gttaacgctg atcatggtgt
      481 ttttgggcgt tggaatgacc ccggcactgc gttccgtcag tgctgaaaaa aatggggctt
      541 ccaaaccacg tcaaaaccag caggaggcac caccggggat ggtcttacgg tacaaaccag
      601 ggatggagcc cattccgggg caggttgagt gggatccagc accagccact gaaccagaac
      661 cgaccagacg tccggacggg agctttacca ccctggtcgg cactgatgac tgccccggca
      721 cgcgaatttg cagtctgccg tttaatgatt cggataccac cgttggggca aataacacga
      781 tcaatacctt accgagtgga tgcaacacct acacgcaagt agctggtcca gataagattt
      841 ataccttgac cgtcggggcg accaactcca tcactgtttc ggtagaccca accggtgcta
      901 actgggatgt gagcgtttac ctggttcgaa cctgccccgc aggaaccgga aacacgatta
      961 catccggaaa ttgtgtcact ggtgacgaca atggaggctc gaataccacc gaaggattta
     1021 ccgtcaccaa tattgcggcg gggacttatt tcctggtcgt ggattcgttt tatcctgcta
     1081 cgggaaccag tccgctaagg cagggaacat acgatttaag tgtcacagga tcagggctgg
     1141 taagtggtga tctatccatc accaaaaccg atggcatcac caccacaacg gctggatcaa
     1201 ccctgaccta tacgattgtg gcttcaaatg caggaggttc ctgtaccgca accggggcca
     1261 cgatagccga taccttctca gctcaattca catctgtatc ctggacgtca gtggctgccg
     1321 gaggtgccac ggggaacgat gcctcaggaa ccggcaatat caatgacact ggggtcaatc
     1381 taccggctgg ggcgtcagta acgtacacgg tgacggcaac cttgagtggc tcggcaacgg
     1441 gttcggtttc caataccgcc accatcacgg caccaggcgg gtttagcgat tccaactcag
     1501 gcaataacag tgccaccgac accgatacga ttatggctgc aggctgtatg gtgacctgcc
     1561 catcaaatgt catcaccaac aacagtgctg gtcagtgcgg agcggtggtg acatttggca
     1621 acccgaccac aagtggatcg tgtggaatcg tgagcgtggt gccaccttca gggtcgttct
     1681 ttacggtggg ttcaaccacg gtgatggtca caacgatggc tggaccgagt tgctcattta
     1741 cagtgacggt caatgatacc gaaccaccca tggtgacctg cccggccaat attgtgaccg
     1801 gagcgacttc gaccgccggt gcggtggtga gctttatgcc gacggtgtgg gacaattgct
     1861 ctggggtttc attcgtttca actccgccat ccggatcaac gttcccgata ggaacgacaa
     1921 cggtgatggt aacggcgacc gatgcctcaa acaacacagc aagttgtagc tttacggtga
     1981 cagttcaggg ggttgaatta gaccgggtgt atgtggcaga tacggccaac aaccggtgtc
     2041 aggtctttga tggcgccgtt tggcaacttg tcccaggcac ggcgggaacg gttggttcca
     2101 gcagtaatca gttccgatca cctgaagcaa tcactgcaaa cgcagatgga tcaaggatct
     2161 atgtggccga taccggaaac aaccgcatcc agtttaccac tgacagcggg gcaacgtggc
     2221 agacgtttgc gacgctggga tccgcctcaa atcaggtgcg ggcaccgcaa gggctggcat
     2281 tggatctgaa tgggaacctg tatgtggccg atactggaaa caaccggttg atgcgatttg
     2341 atggtggtct ccctggacca gccgttatct ttgccacggc aggttcgacg ctgggtaaag
     2401 tgagcacccc acgcggatta gcggtagatg ccagccggac attgtatgtg gcggattttg
     2461 gaaacaaccg aatccagaaa tttacgaccg ccgacgtcac accaacgcca gggctggtgg
     2521 ccaccaccgg gtcaagccag aatccagggc aggtgcgatc acccgaaggg gttggggttg
     2581 acgatgaagg taacctgtat gtggctgaca ccggcaacaa ccgggtgatt gagttcccag
     2641 gtggagctgc tgggccagcc gtgctcttgc tcaacattgg gtcaaccttt ggcaaagccc
     2701 ggttaccaga aggggtaacc gtctgtcagg gagttgtcct gggcggaatg gccacggaaa
     2761 gtgccttgat tgtgggagat accgccaata accgcattca gggaagcctg acacccgctg
     2821 atccattgtc atatgaactg gttggcaatc cactgggcgg cgttggctca ttgatcggta
     2881 acttccggtc accgagcaag attcgctaac tccaggcttg ctgacaaccg ataacaattc
     2941 aaactgggat cactttgaaa aggtgatccc agttcttttt tggcaatcag ttttgcttga
     3001 aacactcaat ccggaatttt caattttttg tggaactttc gaaccggctt ttcaccatac
     3061 ggactgaaac acttaaaaaa gagactcatt gaatttatac catcttggaa tgaattgctt
     3121 gtcttcgcga gtagtgaagt aactcaagaa aatagagtta gtgacctact gactacgggc
     3181 tactgactac aagctggttt gatttgaatt cgtctatcgg tctgatctcg tcaccagata
     3241 ccccaccgag tttgctgtga accacgagac agatcatttc aactcaagat tgcaggagtt
     3301 tccaaaatga aaaaagccca attgaaaccc gttttgttga ttgcgattct ttgttttttg
     3361 atgggtggaa tatcactcac tggatttgcc caacggtctg gcgatggcgg aggccgacat
     3421 aaaagaggac tggctggcca attaaccgcg attgccggct cacaacttac ccttacggcc
     3481 cgcgatgata aatcggtaac ggttctggtt gatgccaata ctcaatatcg ccgcaatgat
     3541 gcggcagcaa ccctggctga tctgcaagtt aatgattatc tgtcagttaa agggaaaccc
     3601 aacgatcaag gtcaggtcct ggcggtagcg atcaatgcca gtaccgagcc tccgcagggt
     3661 ggcggtggcc acgaagggag acgcggccca cgaggtgact ttgtttcagc caacgccgag
     3721 gccggaaccc tgacagtaaa ggtcaaagac ggcacggaag taaccgtcta cacaacggct
     3781 gagaccatta ttctccgcaa ccgggttcaa gccacgttga gtgatttcca ggctggtgac
     3841 cgggtttcag ccgctggccg gaaaaatgaa agcggccagt ttgtggcggt tcgaattatg
     3901 ggtggtgatg ccaaagaagc atcacgtgct gccgctcaaa acaatggcgt cttttttggc
     3961 gaagtcaccc agattgcctc caactcgatt acttttatca accaggacgg ggtggagcaa
     4021 accctcctcg tcagtgaaaa ggcagttgtg actttgaaac atcgtcgggt tgggctggct
     4081 gatttgcggg aaggaatgca catcaatgtg attggtatta ccaatgacca ggggcaagtg
     4141 gttgccaccg ggattttcgg aaactcccac cggggtcatg ggcgacttca aaaataatcc
     4201 gtccttgcca gcagactttg cctctcagtg ctgtgcagtg cctgggtgcc ttgcacagca
     4261 ctcctgtctt gtgaggggaa gccaatcctg gcaacatctt gacctttcgc agcatgccgc
     4321 cgatttgaac aatacaacat ggggattttt tggcgacggt ctgaaccaat cgaaactgat
     4381 gaggcattgc tttgccagat gatggctggt gatgagcagg catttgcccg actctatgag
     4441 cgacgccacc aggacgtgta ccggtttgca ttgcgtatga gcggttccga agccattgcc
     4501 gaagatgtcg cccaggaggt ttttctgacg ttgatccaat cgggaggaaa atttgacccg
     4561 tcacggggaa cagttcaggg atatttgctc ggcattgccc gataccgggt tctgcaccga
     4621 cttgcccagg accgaaccta tgtttcgatg gaaaccccaa ctgatatgag cgagggagtt
     4681 cctgaagaat accgggtcaa tcatcacaat ccactggcgg acctgacgca ttctgagatg
     4741 atcgaacaaa tccagcaggc agtgcttgga ctgccgcccc attaccggga agtggttgtg
     4801 ttatgtaata tggaagaatt gagctatgaa gcggcggctg acatccttga ttgtccggtt
     4861 ggcaccgtgc gatccagact caaccgggcc cggtcgcttt tgcaacaaaa actgtcgtcg
     4921 ctcgcggttc agagttcaag ttttggctcg tgaagctttt accaaaaagc caatgtgggg
     4981 aagcatgtat gaaatgttat gaatttgaag cggttgtgct ggatatcgtg agtggtcggt
     5041 tgatggaagt gcgcctcaaa caggcggccc ttgcccacgc tgaaacctgc ccaacgtgtg
     5101 ggctgcgact tgaacaggaa caacaattga ccgccgggtt gcgggcagtg gcggctcaaa
     5161 ctggcgtggc gaaggcgtcg ccaaaactca accgggagct tcaagcggcc tttcggaacc
     5221 agtttccaaa tcagattcaa aaaccacagc cgcgtcaatt atggaaaaaa cacgagtggc
     5281 tggccctggc cgccgggctg gttctgatgc tggccggcac cgggctaatc tgggttctgg
     5341 ttcaacccgg accagccagt cagattgccg tgagtgcgac cccagacagc ctgtcgcctt
     5401 cacccccaac gatgccggct gaagtgaagc catctgcatc cacgagtcaa ccgtctgtgg
     5461 tgaaacctca agccattccg tctccaatct tcaaaccaca gcgacctcgc cagtcaccaa
     5521 gatcaaaacc aatcaaaccc caaagccggg ttgaaacagt ggatgacttt tcaaatgagt
     5581 ttattccatt gacctatgtt gcggcttcga ccgcgatgga cagtgggcaa atcgttcaga
     5641 tgtacatttc ccgttccacc ttgatttcaa tggggttatc aaccaatgtg gaaaatgcag
     5701 ccgactatgt gaaggctcag gtcatcatga gcgatgacgg tgtgccacgc tctattcgaa
     5761 ttttgccgga atagttgttt ttggttccac tgaaggattt gagagatgaa aaaaggatac
     5821 tggatttgga ttcacatggc agtactcgtc attgcaatgc tggcggcgtc gagtcagatt
     5881 ctgggacagg ggcgtcctgg gcaagatggt ggtggccgac ggccacatcc accatttggc
     5941 ggcgggccag gtcgtggtga ccgtgatcgg ccagggcgtg accgcgagtc tgaccatcaa
     6001 gataggacac gaacaggccg gggcacggga cggtactttg ccgaaatgcg atttgatggc
     6061 aaagtcgtaa ccggcgcacc ctactcagcc cagattgtga cccgcaatac ccaaacctta
     6121 cgtgatggaa ccaaactgac caacgaatca accgccagag tctttcgtga tgcccagg
//
LOCUS       DAOQDO010000053         6121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7764_length_6121_cov_3.093142, whole genome shotgun sequence.
ACCESSION   DAOQDO010000053 DAOQDO010000000
VERSION     DAOQDO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7764_length_6121_cov_3.093142"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PKZ53_01575"
     CDS             <1..1347
                     /locus_tag="PKZ53_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNJ39150.1"
                     /translation="HYSLLVNRKPTITNDGPIPLSDGDLLSLQAVKTSELHLPTELLV
                     HVVQEIEETKTGVRNAHLQFPEVDMGGRIHPRTASRFAKELIIALYAEIPPWARVFAI
                     ILSLVIPLCILVSTGIVFVGVMRYYEILDRFSNLSRAAESQLEDLLTKNEALRQEVDS
                     LKNVLNSGQNIAQAYGPGVCLIEGTYSFTNRQTNKPLRFIDNSFNQSTPLAADGSLQV
                     SFDGTGDIYYEQFTGTGFVVAEGKLVTNRHIAQPWFNDPTDSILINSLGGKPEIKGLN
                     AYFPGIKSPFALKVLQISSENDVALCSFIQGDAAIPQLPLETSEETKNMTGQGIVLLG
                     YPTGVEGIREALPDERLKDELRKRMTFSDLAAELSNRGYIYPKVSQGHITQFITGRIV
                     HDAQTTEGGSGSPIFNSFGKVVGINAQVMLDEQERIQASLGVPISAAVELLKDSEK"
     gene            complement(1513..2286)
                     /gene="lpxA"
                     /locus_tag="PKZ53_01580"
     CDS             complement(1513..2286)
                     /gene="lpxA"
                     /locus_tag="PKZ53_01580"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNJ39151.1"
                     /translation="MAIHPTAIISPRAEIGNGVSIGPFAVIGADVVIADDVEIGSHAV
                     VEGPTHIGSGTRIYPFASIGQEPQDLKYKGERTELIIGERNQIREFATLHRGTVGGGG
                     VTRIGDDNLLMVQSHVAHDCTVGNHVIFANGASLAGHVTVHDRATLGAYVGVHQFCRV
                     GQYAFLGAYSVVVKDALPYARSAGNHAKCYGPNSIGLRRAGFSTEDLRTIERIFRLLL
                     NSKLNTTQAIEAIGQELADNPIACSMVEFIQTAQRGVTK"
     gene            complement(2382..2876)
                     /gene="fabZ"
                     /locus_tag="PKZ53_01585"
     CDS             complement(2382..2876)
                     /gene="fabZ"
                     /locus_tag="PKZ53_01585"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNJ39152.1"
                     /translation="MDIIFDTAQIQDTLPHRYPFLLVDRIIELDPGKRVVGLKNVTIN
                     EPFFQGHFPGLPVMPGVLIIEAMAQTGGMLMLGSVNDPQSKILLFTGIDEAKFRRPVV
                     PGDTLRIEMTLLRFKGKVCKLQGRVFVEDKLAAEAEITSVLVDRSTLRKQSPVSAKET
                     ETPA"
     gene            complement(3033..4082)
                     /gene="lpxD"
                     /locus_tag="PKZ53_01590"
     CDS             complement(3033..4082)
                     /gene="lpxD"
                     /locus_tag="PKZ53_01590"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNJ39153.1"
                     /translation="MSLTFKQLAEFLEVEWTGTPELILERVSPFESAQPGSLCLALGK
                     HREHLADSQGSAFIVDARSVTDADRARFSLLLSPSPKITFAQAIARLHARPAEPPVIA
                     PDFMAGDHTVIGTGVVIHPRVTVGRYSSIGDRTVIHPGVVIGDRVTIGNDCVLFPNVT
                     IYERVTIGDRAIIHAGTVIGSDGFGFAQDKVGRHIKIPQIGDVVIEDDVELGANCTID
                     RATLGTTRIRRGTKFDNMVHIAHNCDIGEDTVIAAQAGISGSVRVGNHVMIAGQVGTN
                     PHVEIGDGAIIAGKSGVSKSVGPGEQASGMPILSLKQWKLMHIFAAQLPDRLPKLEKR
                     LKVVEQTLEQMKNEE"
     gene            complement(4114..4749)
                     /locus_tag="PKZ53_01595"
     CDS             complement(4114..4749)
                     /locus_tag="PKZ53_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ39154.1"
                     /translation="MKKVLFWLFAIVILLPGSALAQQPPISKATTNPGTTPAKPSTPA
                     VIPTGRVAVIDTTQFPKGILEYKKELDKLEAEFQPRTKELETLKTRLDALQKELENAP
                     MMKPEVQQQKVAEYESIKKEAQRKFEDYQADVSKRSGAVLAPLNEKISRFLQGYATSH
                     NIVVVFDMSQNDTTRLFLAINPDSNVTDDFMKEYNKSNPVAAAPPSPAPNK"
     gene            5033..>6121
                     /gene="mutL"
                     /locus_tag="PKZ53_01600"
     CDS             5033..>6121
                     /gene="mutL"
                     /locus_tag="PKZ53_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNJ39155.1"
                     /translation="MSKIQLLPDIVANQIAAGEVVERPASVAKELLENALDAGATRVE
                     VEIEQGGKNLIRIRDDGEGMTRDDTLLAFERHATSKIRTVEDLNAIDTFGFRGEALAS
                     IASVSRITLFSKLRDDVAGTELVIEGGRIRHVRDASWPGGTEISVRDLFFNVPARRKF
                     LKTETTESFHVTNLVTHYALANPEKAFVLKNNGREIIQVTPTASLRDRAYQLFGEALL
                     EELVKVEFSQNEITVGGFISRPQQQRASRDGQYLFVNGRYVKDRVLGRAVSDAYRNII
                     PPGFYPSVLLFVEVSPEEVDVNVHPQKTEVRFRRAQAVLETVRDGLKLALGVEKPFAH
                     FPGLSDLRETETAASVVPPSSEELREPPF"
BASE COUNT         1488 a         1478 c         1607 g         1548 t
ORIGIN      
        1 cattactccc tccttgtcaa tcgcaaaccc actataacga atgatgggcc gatccctctg
       61 tcagatgggg acttgttgtc actgcaagcc gtcaaaacat ctgaactgca cctgccgacg
      121 gaacttctgg tccacgtggt tcaagaaatc gaagaaacca aaaccggcgt tcgcaatgcc
      181 cacctgcagt ttcctgaagt tgatatgggc gggcggattc acccacgcac ggcatcgcgg
      241 tttgcaaaag aattaatcat cgcgctctat gctgaaattc ccccctgggc acgggttttt
      301 gccattatcc tgtcgctggt gatcccgctg tgcatcctgg tttccaccgg gattgtgttt
      361 gttggcgtga tgcgctacta cgagatttta gaccgctttt ccaacctgag ccgggcggcg
      421 gagagtcaac tggaagattt gctgaccaaa aatgaagcgc tccgccagga agttgactca
      481 ctcaagaacg tccttaactc cggacaaaac attgcccagg cttatggacc tggggtgtgc
      541 ttgatcgagg ggacctattc atttaccaat cgccagacca ataagccatt acgctttatt
      601 gacaatagtt tcaaccagag cacaccgctg gcagcagatg gaagtctgca ggtaagtttt
      661 gatggaaccg gcgatattta ttatgagcaa tttaccggta ccgggtttgt ggttgccgaa
      721 gggaaactgg tgaccaatcg gcacattgcc caaccctggt ttaatgaccc gaccgacagt
      781 attttgatca atagccttgg tgggaaacct gagatcaaag ggttaaatgc ttattttcca
      841 gggattaaat ccccatttgc gctcaaagtt ttgcaaattt cctctgaaaa tgatgtggcg
      901 ctgtgtagct ttattcaagg cgatgccgcc attccacaat tgccacttga aacttcagaa
      961 gagaccaaaa acatgactgg tcaggggatt gtgctgttgg gatatccgac gggggtggaa
     1021 ggaattcgtg aagcgctccc tgatgaacgc ctgaaagacg aactgcgcaa acgaatgacg
     1081 tttagcgatc tggctgccga actctcaaat cgtggttata tatacccgaa agtttctcaa
     1141 ggccatatca ctcagtttat taccgggcgc attgtccatg atgctcagac aacggaaggt
     1201 ggctcaggga gtccaatctt taatagtttt ggaaaagtcg tgggaattaa tgcccaggtg
     1261 atgctcgatg agcaggaacg catccaggcc agcttaggcg ttccaattag tgcggcggtg
     1321 gaactgctca aggattctga gaaatgagaa ccatcagtcg ttgatgagtc agtagtcagt
     1381 agtccatcag tcagtagata aaacccagat cgttgaatcc ctgggattat tgaaaaaaat
     1441 tgttcataac tgactcaaat agaaccaaat acacttaaca gatacttcaa aatcccaaca
     1501 tttaactgct gactactttg taaccccgcg ctgggctgtc tggataaact cgaccatgct
     1561 acaggcaata ggattgtcag ccagttcttg cccaatcgct tcaatggcct gagtggtgtt
     1621 aagttttgaa ttgagcaata agcgaaagat gcgctcaatg gttcgcaaat cttcagttga
     1681 aaacccggcg cggcgaagcc cgatgctgtt cgggccataa catttggcat gatttcctgc
     1741 cgaacgggca tacggaagcg cgtcttttac cacgacagag tacgccccaa ggaacgcata
     1801 ctgtcccacc cgacaaaatt gatgaacacc aacataggcg ccgagtgtcg cccggtcgtg
     1861 taccgtgaca tgcccggcca gtgaggcacc attggcaaaa atgacgtgat ttccgaccgt
     1921 acaatcgtgg gcgacatgtg attgtaccat cagtaaatta tcatctccga tacgggtaac
     1981 tccgccgccg ccgactgttc cccggtggag tgtggcaaat tcacggattt gattgcgttc
     2041 gccaatgatg agttccgttc gttcgccttt gtacttcaaa tcctgtggtt cttgaccaat
     2101 cgaagcaaat gggtaaatgc gagtcccact cccaatatgg gttgggcctt cgacaacggc
     2161 gtgagagcca atttcaacgt catcggcaat gaccacgtca gcaccaatca cagcaaaagg
     2221 gccaattgag acgccattgc cgatctcagc gcgtggactg atgattgcag ttggatgaat
     2281 tgccatgcaa aatccttaga atttgtaaat tatgaatgaa gtgcaggtct ttttggtacc
     2341 tgacgctccg aagggcttgt ttcctcagca catgtcccaa ctcaggctgg agtttctgtt
     2401 tctttggctg agacgggcga ttgctttcgc agtgtgctcc gatcaaccag aactgaggtg
     2461 atttctgcct ctgctgccag tttatcttca acaaacaccc gcccctggag cttgcagacc
     2521 ttccctttaa agcgcagcaa ggtcatttca atcctgagtg tatctcccgg aacaaccggg
     2581 cgtcggaatt tagcttcatc aatgccggta aagagcagaa ttttagactg tggatcattg
     2641 actgacccca gcatgagcat tccgccggtt tgagccatgg cttcaataat caaaaccccc
     2701 ggcatgaccg gcagcccagg gaaatgcccc tggaaaaaag gttcgttgat ggtgacattt
     2761 ttcaagccaa cgacgcgctt tcctggatca agttcgatga tccgatccac cagcaggaat
     2821 gggtagcgat gtgggagcgt atcctgaatc tgggctgtat caaaaatgat gtccatatag
     2881 tttttttcaa gattagcaat tggagaaatt ggggaatgaa gaatgaagaa tgaagaatga
     2941 agataatgag tggttcttag ttcttggtac ttggttctca gaagatattg gttccaaagc
     3001 actaagcact aagcactagg cacgagattt cttcattctt cattcttcat ttgctcaagg
     3061 gtctgctcaa ctaccttgag ccgtttttcg agttttggta gccgatctgg gagttgtgcc
     3121 gcaaagatat gcatcagctt ccattgcttc aacgacagaa tgggcattcc ggaggcttgt
     3181 tcgccaggtc caactgattt tgagacacct gatttcccgg cgataatggc gccatcacca
     3241 atttcgacat ggggattggt acctacctgc cccgcaatca tgacgtggtt tccaacgcga
     3301 acgctcccag agatcccggc ctgagccgca atcacggtgt cctcgccaat atcgcagttg
     3361 tgggcgatat ggaccatgtt gtcaaatttg gtgccgcgcc ggatgcgagt ggtcccaagt
     3421 gtggctcgat caatggtaca gttggccccg agttccacgt catcttcaat caccacgtca
     3481 ccaatttggg ggattttgat atgtcggccc actttgtcct gggcaaaccc aaatccatct
     3541 gatccaatca cggtcccggc gtggataatg gcccgatcac caattgtcac ccgttcataa
     3601 atcgtgacat tggggaacag aacgcagtca tttccaatgg tcacccgatc cccaatgact
     3661 acgcctgggt gaatgaccgt tcgatctccg attgaactgt accggccaac cgtgactcgt
     3721 gggtggatga caactccggt gccgatcacg gtgtggtcac cagccataaa atcaggcgcg
     3781 atcacgggcg gttccgccgg tctggcgtgg agtctggcaa tggcctgggc aaaggtgatt
     3841 ttgggggatg gggatagcag gagggaaaat cgcgcgcgat cagcatcagt gaccgatctg
     3901 gcgtcaacaa taaacgcgga tccttgagag tcagccagat gttcgcggtg ttttccgaga
     3961 gcaagacaga ggctccctgg ctgggcagat tcaaatggac tgacacgctc cagaataagt
     4021 tccggggtgc cagtccattc aacttccaga aattccgcaa gttgtttgaa tgtaaggctc
     4081 atgcaggagt ggctgctggc tgttgccctg acgttacttg ttgggggctg gtgatggagg
     4141 cgcagccgcg accgggttgg atttgttgta ctctttcatg aaatcgtcag tgacgtttga
     4201 atctggattg attgccaaaa agagccgggt ggtgtcgttt tgggacatat caaaaaccac
     4261 gacgatattg tgtgaggtgg catagccctg taaaaagcgg gaaattttct cgttcaacgg
     4321 ggccaaaacg gcgccggatc gcttgctgac atccgcctgg tagtcttcaa atttgcgctg
     4381 ggcttctttt ttgattgact catattcagc cactttctgc tgctgaacct caggcttcat
     4441 catcggcgca ttttcaagct ccttctgaag ggcatcaagg cgggtcttga gcgtttccag
     4501 ctctttggtc cggggctgaa actcagcttc aagtttgtcg agttcttttt tgtactcgag
     4561 aattcccttg gggaactgtg tggtatcaat aaccgccacg cgcccggtgg gaataacagc
     4621 cggggtggag ggtttggctg gggtggtgcc agggttggtt gtggctttgg aaatcggcgg
     4681 ctgttgggcc agagccgagc cggggagaag aataacaatg gcaaagagcc agaacaaaac
     4741 cttcttcatt atgtagatcc tcgatgtgtt gcggaattct aaaccctgat aaggagcgat
     4801 caaaacgatg cctggagaaa ggaatatgct actttctgtt ctgagcactg cgatgacgat
     4861 ttacagggat gagtttgtcg aatgtgacaa gattgcagat tatacaaagg gaaatcagaa
     4921 acgcaaacct gattctattc agctaggatg aggagttctc aattccaatt acagtgaaca
     4981 gtttattttg tccagcgacc acgctttttt ggtcattttt tcctggggag ctatgtccaa
     5041 aattcaatta ttgccagata tcgttgccaa ccagattgcc gcgggtgagg ttgtcgaacg
     5101 cccagcttcg gtggcgaaag aattacttga aaatgcactc gatgctggtg ccacgcgggt
     5161 cgaagtggaa atcgaacagg gcggcaagaa tctgattcgc atccgcgatg acggtgaagg
     5221 catgacccgt gatgacacgc tccttgcctt tgaacgccat gcaacaagca aaatccgcac
     5281 cgtcgaagac ctcaatgcca ttgatacatt tgggtttcga ggcgaagcac tggcctcgat
     5341 tgcctcggtg tcgcgcatca cgcttttttc aaaattgcga gacgatgtgg ccggaaccga
     5401 actggtgatt gaaggcgggc ggattcgtca tgttcgtgat gcgtcctggc cgggcggtac
     5461 agagatttcg gttcgtgacc tgttttttaa tgttccggcc cgccgcaagt ttctcaaaac
     5521 agaaaccaca gagagtttcc acgtcaccaa tctggtgacc cattatgcgc tggccaatcc
     5581 tgagaaagcc tttgttttga aaaataacgg tcgtgaaatc atccaggtaa ccccaacggc
     5641 cagtttgcgt gaccgggcat atcagttgtt tggtgaagcc ctgcttgaag aactcgtcaa
     5701 ggttgaattt tcccaaaatg agatcaccgt cggtggcttt atctcgcgac ctcagcagca
     5761 acgggcttcg cgtgatgggc aatatctctt tgtcaacggg cggtatgtga aagaccgggt
     5821 tttagggcgt gcggtttccg atgcctatcg aaatatcatc ccgcctgggt tttatccatc
     5881 agtgctgttg tttgttgagg tttcacccga ggaggtggat gtcaatgtcc acccgcaaaa
     5941 gaccgaggtt cgttttcgcc gggcccaggc cgtgcttgag accgtccgtg atggcctcaa
     6001 actggccctt ggggttgaaa agccatttgc tcattttccg gggttgagcg acctgcgtga
     6061 gaccgagacg gcggcttcgg tggtaccgcc atcttctgag gaattgaggg aaccgccgtt
     6121 t
//
LOCUS       DAOQDO010000054         6103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7801_length_6103_cov_3.312996, whole genome shotgun sequence.
ACCESSION   DAOQDO010000054 DAOQDO010000000
VERSION     DAOQDO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_6103_cov_3.312996"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PKZ53_01605"
     CDS             complement(<1..905)
                     /locus_tag="PKZ53_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNJ39156.1"
                     /translation="MSTQSSVAEIPVSQASKAQENLHTDTTAKYFLISAVAYFFIVGI
                     IALMIAAKFVWPQMLGTVSYLTYGRLRPLHVNGMLFGWLLACDMGLIYYFVPRLCGVK
                     LWSEKLGVATAMLWNFIILSAVVTLLMGLNQGLEYAELVVPLDVLVVVAWVMFGANIF
                     MTIATRKYQQMYVSLWYVMGTILWTAFVYLTGNFAVLMATGVNQANLNWMYVHNAVGL
                     IFTPAGLAIAYYFIPKSSNTPLHSHKLSMVGFWSLAFVYVWTGAHHMLHGPISQWLQT
                     IAIVFSVMLLVPVWAVVYNFFATMKG"
     gene            complement(1101..2927)
                     /locus_tag="PKZ53_01610"
     CDS             complement(1101..2927)
                     /locus_tag="PKZ53_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ39157.1"
                     /translation="MRLALAIASLLILVIHGLVFYDQFFHKWEKFQTAYFDQAKAMAK
                     TDTERAALDGRSPRIEQILVTQFGEMRVDRCTTCHISADDPRFKDYAHPLKSHPFSAA
                     MGDQEINGKWERRHKFSDFGCTVCHDGQGRGLKEYYGHGKDHYWPDPMLGYVAQENWR
                     KEFQPKLVGKEYMEANCAQCHTKENFTATPMITKGRQLFFEKNCYGCHRIEGISNGTL
                     APDLSEVGKKFKIDYLWESVVDPRANLATSVMPKFNLTDDEVKALVVFLKSRRGINYN
                     ETSLERYRARIEEKKPEAAPVAATTGTSPGTAAPSIPPAPEAASALARGEKLIGEKSC
                     VACHKLGDKDGGIAPDLSFEGLLKDGPWLMKHFKNPRAEMPDSIMPGFNLSEADYQAM
                     TDYLLSLKTPPPTATAAETYKNLCARCHGEQGDGKGITWLYLDPAPRDLTKAAFMSSK
                     PDERFIKSIQEGVAGTSMPAWGKTLNETQVRDLVAYIRQSITKEKPRAMAAHQVPDQN
                     PISMTPDSVKRGEQIFLMRCTGCHGKKADGKGPNSLDIIPRPRNLRNQFFVQSMNDRR
                     LFDSILYGVQGTAMPPWIDYGMTKNDVGDVVNYLRSFNQPKK"
     gene            complement(3086..4414)
                     /locus_tag="PKZ53_01615"
     CDS             complement(3086..4414)
                     /locus_tag="PKZ53_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNJ39158.1"
                     /translation="MSNVMKTTSLWNDLLTFPENVWKSIFRNPLPSSDLGRAQTSFTN
                     FFLHIHPVKVHRHTLRPTYTMGLGLMSFFLFVLLVVSGVLLMFYYVPSTSQAYDRMLD
                     LRGTVAFGTFLRNMHRWAAHAMVAVVFLHMCRVFFTGSYKPPREFNWVLGVGLFLLTL
                     FSSFTGYLLPWDQLAFWAITVGTAIAGYAPVVGQDVRFLLLGDSTVGQEALLRFYVLH
                     VAVLPVVITFMVAIHFWRIRKDGGLSRPEVVETPTVQPLQQDSEYVPGATSTVGTPTT
                     EVALTGVEKKRFGLQGLVRGPFAKVGKVDENTVYSWPYLLMAELFVFVLTVAGILVVS
                     LLFDAPLEEPVNIMHPPNPAKAPWYFLGLQELVSYSAFWGGVGIPTIEVLILLVAPYL
                     DRSTKGIGQWFSKDRLLANTLFMTFVVVNIILIIIGTFFRGPNWEFISPW"
     gene            complement(4411..4902)
                     /locus_tag="PKZ53_01620"
     CDS             complement(4411..4902)
                     /locus_tag="PKZ53_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ39159.1"
                     /translation="MPMGKNVISTKPNNTEDTNRRKFLLWLSSFGLFGSAVISAVSNL
                     VFIKPRATYGQPNRFSVGKPDDYPPGTRIALDTHRICIVREENKIAAVSTTCTHLGCI
                     VGLSETGFACPCHGSRFDQDGNVTGGPAPKPLAWYKINLAPNGELEVDKDAEINAGTY
                     YNL"
     gene            complement(4871..5077)
                     /locus_tag="PKZ53_01625"
     CDS             complement(4871..5077)
                     /locus_tag="PKZ53_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39160.1"
                     /translation="MEYTWIAIVASLLMGLAAFLIFMFAVKRDYFRNLEDVKYQVFWS
                     DLEDLVESSKEEPTDAHGQKRHLH"
     gene            5636..5845
                     /locus_tag="PKZ53_01630"
     CDS             5636..5845
                     /locus_tag="PKZ53_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39161.1"
                     /translation="MNHLLSSFNHPTLSFLKDRMVHVMLIGAALFFFALPLNAHAQGL
                     NWEGQTGAFVTIRSLPLPIQLASRN"
     gene            5857..>6103
                     /locus_tag="PKZ53_01635"
     CDS             5857..>6103
                     /locus_tag="PKZ53_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39162.1"
                     /translation="MNTGPIIGHSLQTSVTVGAFKRIEFGYTRAFNAGGNNKAFSALF
                     DSGFNVFHAKVNVVPENVKKANWVPAISVGGVVRSQVQ"
BASE COUNT         1578 a         1575 c         1510 g         1440 t
ORIGIN      
        1 cccttcatcg tggcaaagaa gttatacaca accgcccaca ctggcaccag caacatcact
       61 gaaaacacaa tcgcaatagt ttgcagccac tgggaaatcg ggccgtggag catatgatgc
      121 gccccagtcc agacatagac aaacgccagt gaccagaacc ccaccatcga gagcttgtgg
      181 ctgtggagcg gtgtgtttga tgatttggga ataaagtaat aggcaatcgc aagccctgct
      241 ggggtaaaga tcaagcccac cgcattatgg acatacatcc agttcaggtt ggcctgattg
      301 acacccgtgg ccatcagcac cgcaaaattc ccagtcagat acacaaatgc ggtccacaag
      361 atggtgccca tgacatacca caacgaaaca tacatttgct gatactttcg agtggcaatt
      421 gtcataaaaa tattggcacc aaacatcacc caggccacca ccaccagaac atcaagcggg
      481 accaccagtt cggcatattc cagcccctga tttaacccca ttagcaatgt gacgacggca
      541 ctgaggataa taaaattcca gagcatggcc gtggccaccc caagtttttc actccacaac
      601 ttgaccccgc ataagcgtgg aacaaagtaa tagatcaaac ccatgtcgca tgccagcaac
      661 catccaaaca gcatcccatt gacgtgcagt ggccgcaacc gaccataggt caaatatgaa
      721 actgttccca gcatttgtgg ccagacaaac ttggccgcga tcataagcgc aatgattccc
      781 acgatgaaaa agtaggccac ggcgctgata agaaaatatt tggcagtggt atcggtatgc
      841 agattctctt gcgctttgga tgcctgagaa acaggtattt cagcgacgga tgattgagtg
      901 gacataacca gttgactcct tcgattcagg ggttcaaaac cagggttcaa ggttcagggt
      961 tcagggttca gggtttgaaa accagggttc agggttcagg gttcagggtt cagggttttt
     1021 gaatttaagt cctttaagtc cttttcgtcc ttttcgtctt tttcaaaccc tgacccctga
     1081 cccctgcccc ctacaggatc tcacttcttc ggctgattga aactccgcag gtaattgaca
     1141 acatcaccga catcattttt cgtcattccg tagtcaatcc agggtggcat ggccgtgccc
     1201 tgaaccccat acagaatcga gtcaaacaat cggcggtcat tcatactttg gacaaagaac
     1261 tggttccgca aattgcgcgg acgcggaatg atgtcgagcg agtttggtcc cttgccgtcg
     1321 gcttttttac cgtgacaccc agtgcagcgc atcaaaaaga tctgttctcc gcgtttgact
     1381 gaatcaggtg tcatcgagat tggattttga tctggcacct ggtgagccgc cattgcccgt
     1441 ggcttttctt ttgtaattga ctgacggata taggctacca ggtcacgaac ctgggtttca
     1501 ttgagcgttt tgccccaggc aggcatagac gttccagcga caccttcctg gattgacttg
     1561 ataaaccgct catcgggctt gctgctcata aaggcggctt tggtcaaatc acgtggtgcc
     1621 gggtcgagat agagccaggt aatgcctttg ccatcgccct gttcaccgtg gcaccgggca
     1681 cacagatttt tgtatgtttc agccgcagtg gccgttggtg gcggcgtttt gagtgacagc
     1741 agataatcgg tcatcgcctg atagtcagct tcactgaggt taaaccctgg cataattgaa
     1801 tccggcatct cggcacgtgg attcttaaag tgtttcatca gccatgggcc atctttgagc
     1861 aacccttcaa aggacaaatc cggagcaatt ccgccatctt tgtcgcccag cttatgacag
     1921 gccacacagg acttttcacc aatcagtttt tcgccccgag ccagtgcaga ggcggcttca
     1981 ggcgctggcg gaatggacgg ggccgccgtg ccaggactgg tgccagtagt cgcagcaacg
     2041 ggcgccgctt caggtttctt ttcttcgatg cgggcgcgat agcgttccag cgaggtttcg
     2101 ttgtaattga tcccgcgccg gctcttgagg aaaaccacca gggctttgac ttcatcatcc
     2161 gtcaagttga attttggcat gaccgatgtg gccagattgg cccgtggatc caccaccgat
     2221 tcccacaaat aatcaatttt gaatttcttg ccaacttctg aaaggtccgg tgccagtgtc
     2281 ccatttgaaa tgccttcgat ccggtggcat ccatagcaat ttttttcaaa aaagagctga
     2341 cggcccttgg taatcatcgg ggttgcggtg aaattttctt tggtgtggca ttgggcgcag
     2401 ttggcttcca tatactcttt gccaaccagc ttgggctgaa attccttccg ccagttctct
     2461 tgtgctacgt agcccagcat cgggtccggc cagtagtggt ctttgccgtg gccataatat
     2521 tcctttaacc cacgtccctg accatcgtgg cagaccgtgc agccaaaatc ggaaaattta
     2581 tgacggcgct cccacttgcc attgatttcc tgatcgccca tggcagccga aaacgggtgg
     2641 gatttgagcg gatgggcata gtctttaaag cgcgggtcgt cagccgaaat gtgacaggtt
     2701 gtacaccggt caacgcgcat ttccccaaac tgggtcacca ggatttgttc gatccgggga
     2761 ctgcgtccat ccagggctgc tcgttccgta tcagttttgg ccatcgcttt tgcctggtca
     2821 aaataggccg tctggaattt ttcccacttg tggaaaaatt gatcgtaaaa caccagcccg
     2881 tggatgacca aaatcagtaa gcttgctatt gctaaagcca gtcgcataag ttacctaccc
     2941 tatgatgaat gggattcggg ggtcagagtt cagggttcag ggtttggaaa ttatagaaaa
     3001 ggacataagg gacataaggg acgaaaaaga cataaattca aaaccccgga acccggaacc
     3061 cggaacccgg aacccggctt tcggtctacc agggagaaat aaattcccag ttcggacctc
     3121 ggaagaaggt tccaataatg atgaggatga tgttcaccac gacaaatgtc ataaacaggg
     3181 tgttggctaa taaccgatct ttggaaaacc actggccaat ccctttggta gagcggtcaa
     3241 gatagggtgc caccagcaag atcagcactt cgatggtcgg aatcccgacc ccaccccaga
     3301 acgctgaata actcaccagt tcctgcaacc cgagaaaata ccagggtgct ttggccgggt
     3361 ttggcgggtg cataatattg actggctctt caagcggcgc gtcaaacagg agcgacacaa
     3421 ccagaatccc agccacggtc aggacaaaga caaacaattc agccataagc agatagggcc
     3481 agctatagac tgtgttttca tctactttcc cgactttggc aaacggcccg cgcaccagcc
     3541 cctggaggcc aaagcgtttc ttttcaaccc cagtcagcgc aacttcggtg gtcggagttc
     3601 caaccgttga cgtggcacct ggcacgtact ctgagtcttg ctggagcggt tgcactgtcg
     3661 gcgtttcaac gacttctggc cgcgagagcc cgccatcttt gcgaattcgc cagaagtgaa
     3721 tcgctaccat aaaggtaatg accacgggca agaccgcgac gtgcagcaca taaaaccgca
     3781 acagggcttc ctggccgacg gttgagtctc caagtaacaa aaatcggacg tcctgcccaa
     3841 ctactggcgc atatccggca atcgccgttc caaccgtaat cgcccagaag gccaactggt
     3901 cccacggcaa caaatatccg gtaaaacttg aaaacagcgt aagtaagaac aggccaacgc
     3961 ccagcaccca gttaaattca cgtggtggtt tgtatgaacc agtaaaaaag acccgacaca
     4021 tatgtaaaaa gacaaccgcc accatcgcgt gggctgccca ccggtgcata ttgcgcaaaa
     4081 acgtgccaaa tgcgacggtg ccgcgcaaat ccagcatgcg atcataggcc tgagaggttg
     4141 aaggaacata ataaaacatc aacaacacgc ctgatacgac cagcaggaca aacagaaaaa
     4201 aggacatcag tccaagaccc atcgtgtagg tcgggcgtaa cgtgtgacgg tggactttga
     4261 ccggatgaat gtgcaggaag aaattggtga agctggtttg ggctcttcct aaatcagatg
     4321 acggtaatgg attacggaag atggatttcc aaacattttc cgggaaggtc agcagatcat
     4381 tccagaggct ggtggtcttc ataacattgc tcacagattg taataagttc cggcgtttat
     4441 ttctgcatct ttgtcaactt ccagctcacc gtttggcgcc aggttgattt tgtaccaggc
     4501 caatggcttc ggagctggac caccggtgac atttccatcc tggtcaaaac gtgatccgtg
     4561 gcacggacag gcaaagcctg tttccgacag accaacgata cagcccagat gggtgcaggt
     4621 ggtggacacg gcagcaattt tgttttcttc gcgcacaatg cagatgcggt gggtatcgag
     4681 ggcaatgcga gtgccgggtg ggtaatcatc tggttttcca acgctaaacc ggttgggttg
     4741 cccataggtg gctctgggct tgataaagac caggtttgaa actgcactga taacagccga
     4801 accaaacaac ccgaaactgc tcaaccacaa gaggaacttt cggcgattgg tatcttcagt
     4861 gttatttggc ttagtggaga tgacgttttt gcccatgggc atcggttggt tcctccttcg
     4921 atgattcaac taagtcttca aggtcagacc aaaacacctg atatttgaca tcttccaaat
     4981 tgcggaagta gtcacgtttg actgcaaaca taaaaatcag gaatgcagcc agccccatca
     5041 ggaggctcgc aacgatggct atccaggtgt attccatttt tttgacctca gtgaggcggg
     5101 atcagcggca agaatttggg aatgagagca cgaggggctt gaatctctca ggcaactcac
     5161 tcggcaatcg ctgtgccacg ctcaaaatgg ggaatttcag ttctaaacca ccaggaacag
     5221 ggctggtatg tgtgtgaatt tccacacacc ctatgaaaat ttccacaaac aagcgttcgt
     5281 tttaggctca aaatacctca acccctgctt gccagaggtg tatgagcggg agtgataggc
     5341 cagtcaaaaa gcatttttgg tgagaaaaca gggctggtga acggcgacac atcgccaata
     5401 catcgtcagt caataagtta ctgcgcggtt caaggtctgt gagtaaacac cagagtgcat
     5461 cccaggtaac catccagttc caccggtttc agagatacca cacgtttttc gtgctgctgt
     5521 cccacatcaa cgcgcctttg gtacgtgggt tgcacatctg gaaaccattg caaaaaccaa
     5581 aaattcgacg aaccagagat tgccgcacca tccgctttgc caacaggagc aacctatgaa
     5641 tcatttactt tcttccttca atcaccccac gctgtcattc ctgaaagatc gaatggtgca
     5701 cgtgatgctg atcggagcgg ctctgttttt ctttgcactt ccgcttaatg ctcatgccca
     5761 gggattgaac tgggaaggcc agactggcgc ctttgtaact atacggtctc ttccccttcc
     5821 aattcaattg gcaagccgga attgagcttt cactatatga acaccggtcc gatcattggt
     5881 catagcttac aaacatcagt cacggttggg gcatttaaac gcattgaatt tggctataca
     5941 cgagccttca atgccggagg aaataacaaa gcattcagtg ccctgtttga tagtgggttt
     6001 aacgtctttc atgccaaagt caatgtggtc ccggaaaatg tcaagaaggc aaactgggtc
     6061 ccggccattt cagtaggcgg cgttgttcga tcacaggtcc aac
//
LOCUS       DAOQDO010000055         6054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7900_length_6054_cov_3.533089, whole genome shotgun sequence.
ACCESSION   DAOQDO010000055 DAOQDO010000000
VERSION     DAOQDO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7900_length_6054_cov_3.533089"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /locus_tag="PKZ53_01640"
     CDS             complement(<1..2032)
                     /locus_tag="PKZ53_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ39163.1"
                     /translation="MRHRWLSLLIFGAVTLSTLSFHPFPTHSASYPTVSRTRTQPRQA
                     GNIRIQQANPVVNEQRQLSLTATDASGQPITGVTWESGSPDIASVDPNTGVVSGIQRG
                     FATITARRGSDSISVFVVVARVANAQGAKVPGDTKTDTAGHLYISDPLGNVIFRKENL
                     AMPASIFAGQRGAGARIDGNRLQARFAGPTAVTVNNSADGGIFVADTLNHSLRQIDFD
                     DNVTTILGTGSPGRMSNDVTPFAQAVFNGLRGVVTDSGGNLFVADTDNHAIYQVDFES
                     KQVILLAGQPGTSGIVDGRGRAARFNRPSGLALSPDGKLLIVADTGNNRVRLVARNGT
                     VATLGRKSSARGVLESSASPSFLRPEEHQLLIRRLAAIPAQAENELTFMVPQSVSADA
                     VGNIYVVDQMGASVVTRAFGDLPQKVQLAQDGSFQQAASVQVRGTESFVLDTQAQAET
                     NALKVVSIGAPLINSLSRTSDTLNGGSEVIINGKNFAPESVVILGDRVILDAEVLSAT
                     QIRLRVPAQNAPGNRTLSIQTRGGVAQSSFGIMAKALDSIAKGDITTVAGGVAFIGDG
                     GLAVNASLNLNAFGGGVVEIDGEGNLLIADSGNHRIRQINQADIITTVAGSGNPGLSG
                     DGGPALGAGINFPMSATIDGQGNIIIADSSNNCVRRVDAVTGLMSTIAGTGDY"
     gene            complement(2355..4121)
                     /locus_tag="PKZ53_01645"
     CDS             complement(2355..4121)
                     /locus_tag="PKZ53_01645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ39164.1"
                     /translation="MKKRQFVFGLLVLMVCGITGYGVQEKSEKQSPAATVRTETGQKL
                     DDYLTRLEKFGFAGGILVAKDGAVILEKGYGLANREQNIPNAVDTVFNIGSITKQFTA
                     AAILKLEMQGKLRVEDPINKYLPGVPKDKAGITIHHLLTHSAGLRSDYAETDYEPVGR
                     EEYIKRAMAEPLLSPPGEQFEYANSGYSLLAAIVELISGQSYEQFLYDNLLKPAGMLH
                     TGYCLPKWSPDKQARGYLDGRAWGTVVEKACVGEGPYWALRGNGGIQATVGDMYRWHQ
                     ALEGNTILSAEARRKFQTPYIAEGPQGFSHYAYGWAIMKTPRGTKLVTHNGGNGIFAA
                     DFRRYVDENVVIFIASNADVKAIPASQQIARILFGMNPTFPPKVVEADASKIAKLGGT
                     YTLASGAKLAVAAKSDRLVISPEGQEAFSLLATGTPAVNPRLTEINTRTANLVEQASK
                     GNFELIHQAFGGNIPLASVKARETEMWKNWRERFGEFKGCTVLGTLPMEERMATTARL
                     TFERGEILMRYAWEQGQLVGIRPLPQMTGKAFLPVSPTTFAIYDVMSGDTITIRFETD
                     AAGVASKLIFAGEKEVTAKKTD"
     gene            complement(4246..5676)
                     /locus_tag="PKZ53_01650"
     CDS             complement(4246..5676)
                     /locus_tag="PKZ53_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39165.1"
                     /translation="MHSLRWRLFLMTVAISVVAVLAVGLASSQITSIELNKVVTGTEP
                     LNLEPYRVQLLEHYQKHQSWDQITPLLRQIAQVTERDGLLVDAENQVIAASVPELTQA
                     RITFPEPDKLVVQMIEELPGAVRREVIANEQILIRPPRTVLIDATGKRLGMFFLLPRP
                     EAEPIPPRTRVAAVNRSLFWAVSGIGLLALVATAIVSRRIVGPVEALTAAVHRMEQGD
                     LAQRVTIHSSDEIGQLATAFNRMADSVARSEQLRRTLVNDVAHELRTPLTNIRCQLEA
                     VQDGLLAADTEVVASLHEEVLLLSQLVNDLQDLALAEANQIKLTLAEVELQILIEQAL
                     VSLSPQATARRITISSDVPPDLPAVWADARRVSQILRNLLVNALQHTPAGGTICIQVF
                     KSGNQVELQVTDSGEGIESEHLPFIFDRFYRADPARARAQGGAGLGLAIVKQLVQMHT
                     GHITVTSQKNIGTTFRVTLPITSTPS"
     gene            complement(5676..>6054)
                     /locus_tag="PKZ53_01655"
     CDS             complement(5676..>6054)
                     /locus_tag="PKZ53_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ39166.1"
                     /translation="PRELVARVRAVLRRTIQPLDPRPSVLQFNGLLLDTARHNVWLDG
                     QEIPLTPTEFKILETFVRAPGRVFSRQELVERAFGWDFEGQERTVDAHVMNLRKKMTR
                     PTGKPPLIVTVYGIGYSLSEEKP"
BASE COUNT         1364 a         1614 c         1602 g         1474 t
ORIGIN      
        1 cgtaatcgcc tgtgccggca atggtactca tcaggcccgt cacggcgtcc acccggcgaa
       61 cacagttatt ggaggaatcc gcaataatga tgtttccctg gccgtcaatc gtggcgctca
      121 tcggaaaatt aatacctgcg ccaagcgccg ggccgccatc cccgctcaaa cctggatttc
      181 cactgccagc gactgtcgtg atgatgtcag cctgatttat ttggcgaatg cgatggttcc
      241 ccgaatcggc aatgagcagg ttcccttcgc catcaatttc gaccaccccg ccgccaaagg
      301 cattcaggtt gagactggca ttcacggcca gtccgccatc accaataaat gccacgcctc
      361 cggccacggt tgtaatatcg cctttcgcaa tcgaatcaag tgctttggcc ataattccaa
      421 aggaactttg agccacgccg cctcgggttt gaatggaaag cgtgcgattt ccaggagcat
      481 tttgtgcggg aactcgaagc cggatttggg tggcactgag gacttctgca tccagaatga
      541 cccgatcacc caaaatgacc acactttccg gcgcaaagtt tttgccgttg atgatgactt
      601 ctgagccccc gttcaaggta tcggatgtcc gactgaggct attaatcaat ggcgctccga
      661 ttgatacaac tttgagagca ttggtttcag cctgggcctg ggtatcaagc acaaagcttt
      721 ctgttccgcg cacctgaaca ctggcggctt gctggaagct gccatcctga gccagttgaa
      781 ccttttgcgg aagatcacca aaggcccgtg tcaccactga agcccccatc tggtcaacca
      841 cataaatatt gcccacggca tcggcgctca ctgattgagg aaccataaaa gtgagttcgt
      901 tctcagcttg tgccgggatt gccgccagtc gccggatgag aagttgatgt tcttcaggtc
      961 tcaaaaacga aggcgaggca ctggattcca gcaccccacg ggcgctggat tttcgaccaa
     1021 gggtcgccac ggttccattg cgagcaacca gtcgtacgcg attatttcca gtatcagcca
     1081 cgatcagaag cttgccatct ggactcaggg ccaggccgct gggccgatta aatcgggcgg
     1141 ctcgtccgcg tccatcaact ataccgctgg taccaggttg cccggcgagc aaaatgactt
     1201 gtttggattc aaaatcaacc tgataaattg cgtgattgtc agtgtcagcg acaaatagat
     1261 ttccacctga atcagtcacc acacctcgca atccattaaa aactgcctgg gcaaaaggag
     1321 tgacatcatt ggacatccgc cctggtgaac ctgttccaag aattgtggtt acattgtcat
     1381 caaaatcaat ctgacgcagg ctgtggttga gtgtgtcagc cacgaaaatt ccgccgtcgg
     1441 cactgttatt aaccgtcact gccgtaggac cagcaaaccg ggcctggaga cggtttccgt
     1501 caattcgtgc cccggcgccg cgttggccgg caaaaatcga agccggcatg gccaggtttt
     1561 ctttgcgaaa aataacattt cccagcgggt cactaatata caggtggcca gcagtatcgg
     1621 ttttggtatc acctggaact tttgctcctt gcgcattggc cacacgggcg accacgacaa
     1681 aaacggatat tgaatcactg cctcgccgtg ccgtaatggt ggcaaatcca cgttggatcc
     1741 cgctcaccac tccagtattt ggatcaacac tggcaatgtc ggggctgcct gattcccagg
     1801 tgacaccggt tattggttga ccactggcat cagtcgcggt caatgataac tgacgttgtt
     1861 cgttcacgac gggattggct tgttgaatgc ggatattgcc ggcttgtctg ggctgtgtac
     1921 gtgtccggct gacggttgga tatgacgctg agtgggttgg aaatgggtga aatgagagtg
     1981 tggaaagcgt aactgctcca aaaatgagga gggaaagcca gcggtgacgc ataggaaatt
     2041 ctccaatatt cagttgaaat agatttgagc ttaaaaggtg tggaaatagg agttaccaaa
     2101 ccgtgtaact ctacatccat tcctctaaac tcgcttcttt ttctgcctga actttggtgg
     2161 gaattccctt gaaatccggt tgaaaaccag ttgaaaacca gggctgaaga aagcgggcgg
     2221 aagacatcgg gctcagggct tggggctgaa gacactgggc ggaagacatt gggtgaattg
     2281 ccctgagccc gttttcttcc gccccaagcc tgatgtcttt agctccgagc ttgcgagtcc
     2341 tccgctctgg tatttcaatc ggtttttttc gctgtgactt ctttttcgcc ggcaaatatc
     2401 agtttgctgg cgacaccagc ggcgtcggtt tcaaaccgaa tggtaatggt gtcgcctgac
     2461 atcacatcat aaatggcaaa agtggttggc gagactggca aaaatgcctt gccggtcatc
     2521 tggggaagtg gccggatacc aacgagttga ccttgctccc aggcatagcg catcagaatt
     2581 tcgccgcgtt caaaggttag ccgggccgtg gtggccatcc gttcttccat cggcaacgtt
     2641 ccgaggacag tgcagccttt gaattctcca aaccgctcac gccagttttt ccacatttcg
     2701 gtttcgcggg ctttgacgct ggccagcgga atgtttcccc caaacgcctg atgaatcagt
     2761 tcaaagtttc ccttgctggc ctgttcaacg agatttgcgg tgcgggtgtt gatttcggtc
     2821 aagcggggat tgaccgctgg agttccggtt gcaagcaggg aaaaggcttc ctggccttcc
     2881 ggtgaaatga ccagtcggtc tgatttggcc gcaactgcca gtttggcgcc tgaggcgagg
     2941 gtgtacgttc ctccaagttt ggcaattttg ctggcgtcgg cttcaaccac tttgggcgga
     3001 aacgttgggt tcattccaaa caaaattctg gcgatttgtt gtgaggctgg aatggctttc
     3061 acgtcagcgt ttgaagcgat aaaaatcacc acgttttcat ccacgtagcg ccggaaatca
     3121 gcggcgaaaa tcccgttgcc gccattgtgt gtcacgagtt tggtgccacg tggcgtcttc
     3181 ataatcgccc aaccataggc gtaatgcgaa aatccttgcg gtccttcggc aatgtacggc
     3241 gtctgaaatt tgcgtctggc ttcggctgaa agaatcgtgt ttccttccaa tgcctgatgc
     3301 cagcggtaca tatcgccgac ggtggcctgg atcccgccgt ttccacgtaa cgcccaatag
     3361 gggccttcac caacacaggc tttttcgacc accgtgcccc aggctctgcc gtccagatac
     3421 ccacgcgcct gtttgtctgg cgaccatttg ggcaggcaat atccagtgtg gagcatcccc
     3481 gctggtttca acagattgtc atacaaaaac tgttcgtagg attgacctga aatcagttca
     3541 acaatcgctg ccagcaggct gtatcctgaa ttggcatatt caaactgctc tccgggaggg
     3601 cttaacagtg gttctgccat tgcccgcttg atatattctt cgcgtccgac tggctcgtaa
     3661 tctgtttcag cataatcact tcgcagtccg gcgctgtgag tcagcaggtg atgaatcgta
     3721 atcccggctt tgtcttttgg tacacccggc aggtatttgt tgatcgggtc ttcaacccgg
     3781 agtttgccct gcatttcaag cttcaaaatt gcagcggcgg taaactgttt ggtaattgag
     3841 ccaatgttga aaaccgtgtc cactgcattt ggaatatttt gttcgcgatt ggcgagccca
     3901 tatccttttt ccagaatcac ggcgccatct ttggcaacga ggattccgcc ggcaaaccca
     3961 aatttctcaa gtcgcgtgag atagtcatcc agtttttgac cggtttcggt gcgaaccgtc
     4021 gcggcgggtg attgtttttc agatttttcc tgaactccat atccggtgat gccacacacc
     4081 atcagaacca gtagcccaaa cacaaattgg cgctttttca tatgatgaga ccttcctgtt
     4141 gttcaatggg agataccacc tcacctctgg cgttgttgtt tcgcctggtg atggccattt
     4201 tggtgaagtg tgttgaggga aaagtagcgg tgaatcttga agaacttatg aaggtgtcga
     4261 ggtaatcggc aatgtcacgc gaaaggtggt gccgatgttt ttctgactgg ttacagtgat
     4321 gtgtccggtg tgcatctgca ccagttgctt gacgatggcc agccccaggc cggcaccccc
     4381 ttgcgcacgg gcacgcgccg ggtcagcccg gtaaaaccga tcaaaaataa agggaaggtg
     4441 ttcagattca ataccttccc ccgaatcagt cacctggagt tcaacctggt ttccgctttt
     4501 gaaaacttga atgcaaattg tcccgccagc cggtgtgtgt tggagtgcat tcaccagcag
     4561 attgcgcaaa atctggctca cccgccgggc atcagcccaa accgcaggca ggtcaggtgg
     4621 cacatctgaa gaaattgtga tccttctggc tgtggcttgc ggcgaaagcg aaaccagtgc
     4681 ctgttcgatc aaaatttgaa gctcaacttc ggccagggtc aatttaatct gattggcttc
     4741 agccagtgcc aggtcctgta aatcattgac aagctggctg agcagcagca cttcttcgtg
     4801 aagtgaagcc accacttcag tatcagccgc aagcaatcca tcttgaaccg cttcaagctg
     4861 gcagcggata ttggtgagtg gggtgcggag ttcgtgagcc acatcattga ccagtgttcg
     4921 ccggagttgt tcagatcggg caactgagtc agccatgcgg ttaaaggctg tggcaagctg
     4981 gccgatttca tcagatgaat gaattgtcac gcgttgggcc aggtcgccct gttccatgcg
     5041 gtgcacagca gcggtcagcg cttcaaccgg acccacgatt cggcgggaga caatcgcggt
     5101 tgccaccagc gccaggagtc caatcccgct caccgcccaa aacagtgaac gattgaccgc
     5161 tgccacgcgg gttcgaggtg gaatgggttc tgcttcagga cgtggcaaga gaaagaacat
     5221 cccaagccgt ttccccgtgg catcaatcaa aacggtacgc ggcgggcgaa tcagaatctg
     5281 ttcattggca attacttcgc gcctgacggc acctgggagt tcttcaatca tctgcaccac
     5341 cagtttgtca ggctcaggaa aggtgatgcg cgcctgagtc aattctggca ccgaagccgc
     5401 aatcacctgg ttttcagcat ctaccagtaa tccatcccgc tctgtgacct gggcaatctg
     5461 ccgcaaaagc ggggtgattt gatcccacga ctggtgtttt tgataatgct caaggagttg
     5521 cacccgatag ggttccaggt tgagtggttc agtaccggta accaccttat ttaattcaat
     5581 ggacgtaatc tgactcgacg ccaggcccac cgccagcacc gccaccacgg aaatcgccac
     5641 tgtcatcaaa aataagcgcc aacgcaggct gtgcatcatg gtttttcttc agacagggaa
     5701 tagccgatcc cgtaaaccgt cacaatcaac ggcggcttcc cggtcggtcg ggtcatcttt
     5761 ttgcggagat tcatcacgtg cgcatcaact gtgcgttcct ggccttcaaa atcccagcca
     5821 aatgcccgtt cgaccagttc ctggcgtgaa aaaacccgtc ccggcgcccg aacaaacgtt
     5881 tccagaatct tgaactcggt tggtgtgagc gggatttcct gtccgtcgag ccagacattg
     5941 tggcgtgcgg tatccagcag caatccgttg aactgcaaca ctgacgggcg cggatcaagg
     6001 ggttgaatcg tccgcctgag tacagcccgg acgcgggcca ctaactcgcg tggg
//
LOCUS       DAOQDO010000056         6027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_7958_length_6027_cov_3.106330, whole genome shotgun sequence.
ACCESSION   DAOQDO010000056 DAOQDO010000000
VERSION     DAOQDO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_6027_cov_3.106330"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2667)
                     /locus_tag="PKZ53_01660"
     CDS             complement(<1..2667)
                     /locus_tag="PKZ53_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39167.1"
                     /translation="MKVFFPLAKRSFSLALFLLIGFCLWFTSAQPHISAQATPQISTV
                     SIKNTTLLVKGQNLTAAAIEVDGVRLDDTKAAASGKKLKSKQGGTLAPGSYIVTVRTQ
                     SNQRSAPVIFDNQVSQPPKVTLALTAVSVQLPAGTTLQVTATARDAAGGELTGVNYTW
                     QSTNPAVATVSPTGVVSGLAEGTTQVVVSALRNEALLEIKVLGGESGTPASITVIPAN
                     PVLNETETLQLKAEVRDASGNVVADAPVTWSSDNTSQVAIDAGGTVQAVKRGFAAVTA
                     KSGTLQTIVSVAVVKVESGNPASSGGPGEVRTDLANRVYQTDLAQHVVRRGEFGKPLE
                     VYTGEVNAPGRSDGTLTQARYNGPLGIGIDNQDGDVFIADTANQVIRSISSRNGRVVT
                     LAGISGQAGYRDGPLDQALFNSPRGVVMSSDGNVYVADGLNHVIRVIDMRRKEVRTLA
                     GSPGVSGLADGSKGNARFNQPQGLSLDTSNRNIVVADTANNRIRLVTPEGQVLTLGAL
                     TGSRKPVYNQWQHRSSATLTAPGSDFQKTFQAQGDTSLVLDQPRAVSVDKIGTIYVTD
                     SQGVQVLPTRGGRALGQFPLADSRTLGSVSGVTVASVEGNFAGGTRANAVVVNSQVNG
                     QSQLVRVTVGVPSISSILPQSAPVGGGTEVTVAGVNFTPDMQVFLDGAEVNTLNIEGS
                     TQFTFVTPVLTESGLRTLTVQGRGGSRQFPFFLKPPSFDDIGPGDITTFVGGTTYVGD
                     GGEAVKCTLAGPSGLSIDTEGNLLLVDSYNNRIRQVDFQTNLITSIAGRGTLGATGDG
                     GSALQASLGTPEDVITDFDGNFFVSDSGNFRIRRIDAVTGKISTYAGGEEGFGGDGGP
                     ATEAKLGQPAGLALDEEGNLYITDRIKDLMKTS"
     gene            complement(3113..4732)
                     /locus_tag="PKZ53_01665"
     CDS             complement(3113..4732)
                     /locus_tag="PKZ53_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNJ39168.1"
                     /translation="MLGQMMDYPLTITSLLKRAHKLFPDREIVTRLPSVAMHRYTYGD
                     CYRRVCQLANALKRLGMEPGERVGTFAWNNYQHLELYFAVPCSGAVIHTLNIRLSPDQ
                     LAYVINHAENKTIFVDYCLLPLLERAVDQIKTVRQYVLIGAPPDVETSLSPILHYEDL
                     LAGETDDFRWPELDERSAAGLCYTSGTTGNPKGALYSHRSQFLHSVVAALGDGIGICN
                     RDSVLAVVPMFHANAWGLPYTCAMLGTRLVLPGPHLKPADLAELIQDEKVTVAAGVPT
                     LWIGLHQHLKAHRYDISSLRVLLIGGAAAPLGLIQGFEEDFNVPVMHAWGMTELSPMG
                     TNSGLLNDQPTLTREERYQRKLRQGSPVPGVELRIVGEDGKELPWDDKAVGDLQVRGP
                     WVVSSYFRDPSGDAQFTEDGWFITGDVARINPQGSMQLTDRSKDLIKSGGEWISSVAL
                     ENALMSHPKVFEAAVIAIPNEKWTERPLAVVVPQPGQIPTHDELIHHLADQFAKFWLP
                     EEIRLVPEIPKTSVGKFDKKVLRQRYADGNL"
     gene            4964..>6027
                     /locus_tag="PKZ53_01670"
     CDS             4964..>6027
                     /locus_tag="PKZ53_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ39169.1"
                     /translation="MNSQFTTFKRRIVSVLLILLCITTLTPAVPAAFVEENPYNKFRN
                     RERGKGAMSQEDELKFAAAAHDEIAKTNRFVTDQALVGYLNDLGKRIAAKSGRPDLPY
                     QFYLIENDQINAFTPGGGRVYVYTGLVKSTTSEGQLASVLAHEIGHNVGYHVSDQIKR
                     AQTLGILAGIAGAILGNGALGNVTQLATQLLAGGYLFKRSRDAEREADYLGLYDLKDT
                     GYNTDEMNNMFRLLASLGKQPGALEKAFASHPPAAERLANTEREIKENLPGTNQRGIR
                     STTAYDTMKRRLTGNASNTADSGNTSSGSSADTVAVRRDAEQLLSKMRESGARVLQAV
                     RGDVTGDGGNDLVVVYDRRG"
BASE COUNT         1500 a         1646 c         1525 g         1356 t
ORIGIN      
        1 agaagttttc attaaatcct ttattctatc ggtaatatag agatttcctt cttcatccaa
       61 cgccagacca gcaggttggc ccagtttggc ttcggtggcc ggacctccat caccgccaaa
      121 tccctcctcg ccaccagcgt atgtcgagat ttttccggtc acggcatcaa tgcggcgaat
      181 gcggaaatta cctgaatcac tgacaaagaa attgccgtca aagtcagtga tgacatcttc
      241 aggcgtaccg agactggctt gcagggccga cccgccatcg ccagtcgccc cgagtgtgcc
      301 acgcccggca atcgaggtaa tcagattggt ttgaaaatcc acctgacgga tgcggttatt
      361 gtagctatcg accagcagta aatttccttc agtatcaatt gacaacccag aaggcccagc
      421 cagggtgcat ttaacggctt caccgccatc accaacatag gtcgtcccgc ccacaaacgt
      481 ggtgatgtcg cccggaccga tgtcatcaaa ggatggaggc tttaagaaaa atgggaattg
      541 tcggcttccg ccgcgccctt gaacggtcaa ggttcgcaac ccgctttcag tcaagacggg
      601 tgtgacgaat gtaaactggg ttgaaccttc gatattgagg gtgttgacct ccgcaccatc
      661 cagaaagacc tgcatgtcag gtgtgaagtt gacacctgcc accgttactt ccgtgccgcc
      721 tccgactggg gctgactgag gcaaaatact tgaaatgctg ggcaccccaa ctgtcacccg
      781 gaccagttgg gactggccat tgacttgtga attgaccacc acggcattgg cgcgggtacc
      841 gccagcaaaa ttgccttcaa cactggcaac cgtcacaccg ctcacgctcc ccaatgtgcg
      901 cgaatcagcc agtggaaact gccctaaagc ccgcccgccg cgcgttggaa gaacctgaac
      961 gccctgactg tcagtcacat agatcgttcc aatcttatca acgctgacag ctcgcggctg
     1021 atcaagcacc agtgaggtat ccccttgcgc ctgaaatgtt ttctggaaat ctgaacctgg
     1081 cgccgtcaat gtggctgatg accggtgttg ccactggtta tagactggtt tccgggatcc
     1141 agtaagggca ccaagggtta agacctgccc ttcgggcgtt accagtcgaa tccggttgtt
     1201 ggcggtatca gccaccacga tgttgcggtt tgaagtatcg agcgacagcc cttgaggctg
     1261 gttaaagcgg gcattgccct ttgatccatc agccaacccc gaaacccctg gcgaacccgc
     1321 cagcgttcgg acttcctttc gacgcatgtc aatcacgcga atcacatggt tgagcccatc
     1381 agcgacatac acattgccat cgctcgacat caccacacca cgtggcgagt taaataatgc
     1441 ctggtccagt gggccatcgc gatacccggc ctgaccagaa ataccagcca gcgtcacgac
     1501 gcgaccattg cgactgctga ttgagcgaat gacctgattg gcggtgtcgg caataaaaac
     1561 atcaccatcc tgattatcaa tcccgatccc aagcgggccg ttatatcgag cctgagtcag
     1621 tgttccatct gaacggccag gagcattgac ctccccggtg tagacttcca ggggtttgcc
     1681 aaattcgcca cgccgcacca cgtgttgggc cagatcagtt tgatagaccc ggtttgccag
     1741 gtcggtccga acttcccccg gaccacctga gctggctggg tttccagatt caaccttcac
     1801 caccgccacc gaaacaatgg tttggagggt tccacttttg gctgtgaccg ccgcaaatcc
     1861 gcgcttgacg gcttgaaccg ttccaccagc atcaattgcc acctgcgaag tattgtcgga
     1921 actccacgtc acaggtgcat cggcaaccac attgccagac gcatcgcgca cttcagcctt
     1981 gagttgcaac gtttcagttt cattgagaac tggattggct ggaatcaccg tgatgctggc
     2041 tggggttcct gattcaccac caagcacttt tatttccagc agggcttcgt ttcgcaaggc
     2101 agagaccacc acctgagttg taccttccgc cagaccagag accaccccgg tggggctcac
     2161 cgtggcgaca gccgggttgg tactctgcca ggtgtaattg actccagtca gttcgccacc
     2221 agccgcatcc cgagctgtcg cagtaacttg caatgttgtg ccagccggca actgaacact
     2281 gacagccgtc agagccaggg tgactttggg tggttgtgaa acttgattgt cgaaaataac
     2341 tggggctgac cgctgattac tttgggtgcg aacggtcaca atatacgagc ctggggccag
     2401 ggttccacct tgtttggatt tgagtttttt tccagaagct gccgctttgg tatcatcgag
     2461 tctgacacca tcaacttcga tggcagcagc cgtcaggttc tggcctttga ccagcaacgt
     2521 agtgtttttg attgaaacag ttgaaatctg aggtgtggcc tgggctgaga tatgaggttg
     2581 agcggaagta aaccagaggc aaaaaccaat gaggagaaaa agagcgaggg aaaaagatcg
     2641 tttggcgagt ggaaagaata ccttcataca aactcctcac aaaattgagc aacaatgtgt
     2701 ggcgcgtcga agggtagtgc caaagatcgg agaaggatta cccagcagtc agaacgaact
     2761 caatcccacc gaaaacactt ccaatctttt tcagactgct gggtctcctt caagacgccg
     2821 agagaataca caagattttt ccagacgtaa gcatctttgg atatccggca ccactgtcgg
     2881 acaagcggtt tcattttcga cctgaaaggc acagtctgaa cccccaataa gttcagtttt
     2941 ggagattcca aaatagagag caacctctgt tccagtcctt cgtgtgacag gattgctcgc
     3001 cttttcagct ttcaaagact cagttccgcc aaaaccgagt cactttgaca cacacacctg
     3061 aaacaaaatg tgccatacca aagcggtgtc aagccaccgc acgccaaaag aattacagat
     3121 ttccgtcggc atagcgttgc cgaagaacct tcttgtcaaa ttttcccaca ctggtcttcg
     3181 gaatttcagg taccagccgg atttcttcgg gtaaccagaa tttcgcaaac tgatccgcca
     3241 gatggtgaat caattcatca tgggttggaa tttgccctgg ctgaggcacc acaaccgcca
     3301 gcggtcgctc agtccatttt tcattgggaa tggcaatgac cgccgcctca aacactttgg
     3361 gatgtgacat gagggcattt tcaagcgcga cactggaaat ccactcgcca ccgctcttga
     3421 tcaggtcttt gctgcgatct gtgagctgca ttgagccctg agggttgatc cgggcaacgt
     3481 cgccggtgat aaaccagcca tcctcagtaa actgggcatc cccgctagga tcacggaaat
     3541 aggatgaaac cacccacggg ccgcgaacct gtaaatctcc aacggcttta tcgtcccagg
     3601 gaagttcttt cccatcttca cccacaattc gcagttcgac gccgggaaca ggcgacccct
     3661 gacgaagctt gcgctgatag cgttcttcac gggtcagagt gggttgatca ttgagtagcc
     3721 ccgaattggt gcccatcgga gacaattccg tcatccccca ggcatgcatc acaggaacat
     3781 taaagtcctc ttcaaatccc tgaatcaggc caagtggcgc cgccgcgcca ccaattaaca
     3841 acactcgcag gctgctgatg tcataccggt gggcttttaa atgctggtgg agaccaatcc
     3901 agagcgttgg caccccagcc gcaaccgtta ccttttcatc ctgaatcaac tcagccagat
     3961 cggctggttt gagatgtggc cccggcaaga ccagtctcgt accaagcatg gcacaggtat
     4021 agggcaatcc ccaggcattg gcgtgaaaca ttgggacaac cgccagcacc gaatcgcgat
     4081 tgcaaattcc aatcccatcc cccagggccg ctacaaccga gtgcaaaaac tgtgatcggt
     4141 ggctatagag cgcccctttg ggattgccag ttgttcctga ggtgtagcac aacccggcag
     4201 ccgaacgttc gtccagttcg ggccagcgga aatcatcggt ttcaccagct agcaaatcct
     4261 catagtgcaa aattggcgaa agtgatgtct cgacatcagg tggagcaccg atcaacacgt
     4321 actgacgaac cgttttgatt tgatcaacgg ctcgctcaag caacggaagc aggcagtaat
     4381 caacaaaaat ggttttgttt tcagcgtggt tgatgacata ggccagttgg tcgggggaaa
     4441 gccgaatatt gagcgtgtga atgacggccc cgctacaggg cacggcaaaa tacagctcca
     4501 ggtgttgata gttattccag gcaaatgttc cgacccgctc acccggttcc attccaaggc
     4561 gcttgagagc attggccagt tgacacaccc gacgataaca gtcgccatag gtatagcgat
     4621 gcatggcaac cgaaggaaga cgagtgacaa tttcacgatc tggaaagagt ttgtgtgcac
     4681 gtttcaaaag tgaagtgatg gtcaacgggt aatccatcat ctgtccgagc ataattcgaa
     4741 ggctccttgt gcagtcaaat tttggcttaa aaatgcaatg gaagtactca ccccagatgc
     4801 atcaaagcgg cattgtcatc agagtgagtc aagatattgg gtaaggtggg gaaaaacgat
     4861 ttagacgtgt gtggttctaa actgctatca tcgctgcaac cctgctttta atcaatgtca
     4921 tcgtaccgct acatatctca cttatctcca agggacacag cctatgaact ctcaatttac
     4981 cacatttaag cgccgtattg tctccgtttt gttgatttta ttgtgtatta ccactctcac
     5041 accggctgta cctgccgcct ttgtcgaaga gaatccttat aacaagtttc gcaatcgcga
     5101 gcgaggaaaa ggggcgatga gccaggagga tgaattaaag tttgccgccg ctgctcatga
     5161 tgaaattgcc aagaccaacc ggtttgtcac tgaccaggct ctggtcggat atctcaatga
     5221 tctcggcaaa cgcattgccg ccaaatcagg acgcccggac ctgccatatc agttttatct
     5281 gattgaaaat gaccagatta atgcctttac cccaggtggt ggaagggtct atgtctatac
     5341 aggtctggtc aaaagcacaa cgtctgaagg ccagcttgcc tccgtgctgg ctcacgaaat
     5401 tggccataat gttggctatc acgtcagcga ccagatcaaa cgcgcccaaa ccctggggat
     5461 tttggctggg attgccggcg ccatcctcgg aaatggggca ctgggcaatg tcacccaact
     5521 ggcgacacaa ttgctggctg gtggatactt atttaaacgt tcgcgggatg ccgaacgtga
     5581 agccgattat ttagggctct atgatttaaa agacactggc tataacaccg atgaaatgaa
     5641 caatatgttc aggttgctgg cttcattggg caaacaacct ggtgcccttg aaaaggcatt
     5701 tgcttcgcat ccaccagccg ccgaacgact ggccaacact gagcgggaaa tcaaagaaaa
     5761 cctgccagga accaaccagc gtggtattcg gtcaaccacc gcctacgaca cgatgaaacg
     5821 acggctgacc ggcaatgcca gcaacacggc tgatagcggg aacacttcaa gcggaagcag
     5881 cgccgatacg gtggctgtgc gccgcgatgc cgagcaactc ctttccaaaa tgcgtgaatc
     5941 tggagcacgg gtgctgcagg ctgtccgtgg cgacgtgacc ggcgatggcg gcaatgatct
     6001 ggtcgtggtg tatgaccgcc gcggcat
//
LOCUS       DAOQDO010000057         5924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8204_length_5924_cov_3.047197, whole genome shotgun sequence.
ACCESSION   DAOQDO010000057 DAOQDO010000000
VERSION     DAOQDO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_5924_cov_3.047197"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..567)
                     /locus_tag="PKZ53_01675"
     CDS             complement(70..567)
                     /locus_tag="PKZ53_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39170.1"
                     /translation="MRTCLIRLFLFCGAMILASIPTLAQDTPKAELFAGYSAARFANR
                     GDYLSGFSVSINGNFTRSVGVVADFGTYFKNNDQIYNFMAGPRFSYRKSSRVTPFAQV
                     LAGVVAPEPAFTMAIGGGIDIKLNEHIAFRAVQAEYLFLRGGENATNHNARISTGIVF
                     RFGKI"
     gene            complement(789..2912)
                     /gene="dnaX"
                     /locus_tag="PKZ53_01680"
     CDS             complement(789..2912)
                     /gene="dnaX"
                     /locus_tag="PKZ53_01680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNJ39171.1"
                     /translation="MSYQVIARKWRPQAFEEVTGQEAITRTLRNAITGNRLHHAFLFA
                     GPRGVGKTTTARLLAKALNCQTGPTATPCGQCSSCLEVTNGNSIDVLEIDAASHTGVD
                     NIRDVIISTVANRPARDRYKIFIIDEVHMLSISAFNALLKTLEEPPSHVIFIMATTEL
                     HKVPETILSRCQQFEFRTISTEKIAERLRLIGEAEHITISQDALLRIASAGRGSMRDA
                     QSAFDQVIAFTGTTIEEKDVLASLGLIGPQFVGQVIDALVARDGKQMLTLVDELVRTG
                     YDLRQFLHEVMTYLRHMLVFQAAGADPELLPVSESEGRQIEQHARTFSSEDLVRFFTL
                     LTEVEQALRTAPEPRFQLEFGLVKLTQLAHLKSLETLLAQLEKLIGSGGMPSPAPSTP
                     PPPPAGSGGRGPAAPSGSRGNPPPAPSRPMPPPRPQFSGPSGGAPTPPQFSGASAPPI
                     APPKFSGASTAPPAGSRPTFPPPSLSPEITNRLPVAQTARPALAPTVATPMVPSPSVE
                     PPPPDLPPWMDDLTAPPPEPPDDFDESPTLTPPPPQGSSSRTTQPPPVRSPLSLVTPA
                     DQPSSRLDLKADGALEALLERLRADFETSKKPLLLMTLDKVVRAELDGTEVTLLLPSD
                     AVEAHKQLSLPPNLMALQDALRRMTGMPYRPRVKFEKTATSRPTALSESEVTASRSRY
                     QAESDPVVQALLQTFRGELTQVKPG"
     gene            complement(3059..3157)
                     /gene="ffs"
                     /locus_tag="PKZ53_01685"
     ncRNA           complement(3059..3157)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKZ53_01685"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3308..3535)
                     /locus_tag="PKZ53_01690"
     CDS             complement(3308..3535)
                     /locus_tag="PKZ53_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39172.1"
                     /translation="MANAIASLFLLAFIGIWGVIGVAFIIRGLWLSLGLESPTTIDWS
                     QLNIAKNLFENPQYFDAISAFSRVSARLMSF"
     gene            4106..5485
                     /locus_tag="PKZ53_01695"
     CDS             4106..5485
                     /locus_tag="PKZ53_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ39173.1"
                     /translation="MKIIFTHLNGSREGQVEEYTLPVINVGRGVESDIRIGDATPTDR
                     VASRQHAEFRREGNHLILYDLGSRNGTYVNGQRVNGQIELATHDLIELGIGGPAMRVQ
                     VVVSETEEIDFLQNSRYFGSLSQEHLSMVLSAGTLEVYPADSYLFRVGQACEYLYVIK
                     SGVVEVWRDTNENGELDLTTYLGSGDVLGASSILLEGATHHSAARIPEGAVMLRLTQS
                     VFRTLILTIPEFALAVCTALARQLDSTYKKLQTHSYKRLQGNLNFFDLATVIQTLIQS
                     RQTGVLSVTALEVGFAHSGLWASSDDIEMPAATIHLVNGKVVYTRCGVLNGEEAFYQL
                     FQSEYHGSFTFQEGAPPDSGSPQGTIHLPGFNLLLEAVRMQDELRQFKLKHPDPHVKY
                     IPATQSLTWTESMYDKAAHVIWERLKRGASIANLQGEVPYCDFVIYFVIDVLLEKGLL
                     SVSGEGSRS"
     gene            complement(5559..>5924)
                     /locus_tag="PKZ53_01700"
     CDS             complement(5559..>5924)
                     /locus_tag="PKZ53_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNJ39174.1"
                     /translation="GHDSELMQMASELALCHHEKWDGSGYPRGLKGEEIPLIARIVAL
                     CDVFDALTSARPYKKAWSVEETMAYIEGERGKHFDPKLVELFQQVLPEILEIKARYAD
                     PVVPRPTHHSLSERPVKIE"
BASE COUNT         1392 a         1406 c         1660 g         1466 t
ORIGIN      
        1 aacccatttt cttcagcccc gagcttgcga gtcttcagcc cttagcccgt tttcttcagc
       61 cctggttttt cagatttttc caaagcgaaa gacaattccg gttgaaatgc gggcattgtg
      121 attggtcgcg ttttcccctc cgcgaaggaa caggtactct gcctgaacag cgcggaaggc
      181 aatgtgttca ttgagtttaa tatcaatgcc tccgccaatt gccatagtaa aggctggctc
      241 tggggcgacc acaccagcca gcacttgcgc aaatggtgtt acccgcgagg atttgcggta
      301 agaaaaccgt ggcccagcca taaagttata gatctggtca ttattcttaa aataggtgcc
      361 aaagtcagcc accacaccaa ctgaccttgt aaaatttccg ttgatcgaaa ccgaaaaacc
      421 actcaagtaa tcgccgcgat tcgcaaatcg agcggctgag tacccggcaa aaagttcagc
      481 tttgggtgtg tcttgagcca gtgtcggtat ggatgccaga atcatcgcgc cgcaaaagag
      541 aaagagacga atcaaacagg ttcgcattgg ggggaagtcc tttcaaaaag aatatcgggc
      601 tgaaaacttc gggctgaaga caacgggctt ggggctgggg gctgaagagg tcgggttgaa
      661 gaagtggggc tgaagaatat cgggctcagg gtattgaacc cattttcttc agccccgagc
      721 ttgcgagtct tcagccctca gcccgttttc ttcagccccg agcttgcgag tcttcagccc
      781 ccggattttc agcctggctt gacctgggtc aattcgccgc gaaaagtttg cagcaacgcc
      841 tgaacgacgg ggtcactttc tgcctggtac cgcgagcgcg aagcggttac ctctgattct
      901 gagagggccg ttggacgact ggtggcggtt ttctcgaact taacccgggg gcgatatggc
      961 ataccggtca ttcgccggag cgcatcctgg agggccatca ggttgggcgg cagtgaaagc
     1021 tgtttgtggg cttcaaccgc gtctgaaggc aagagcaatg tgacttcggt tccatccagc
     1081 tccgcccgca ccactttatc gagtgtcatg agcaggagcg gttttttgga tgtttcaaaa
     1141 tcggctctga gtcgctcaag caaggcttca agtgcgccgt ctgcttttaa atcgaggcgg
     1201 gatgacggct gatcggctgg tgtcaccagt gagagcggag acctcacggg cggtggctga
     1261 gttgttcgtg aactcgaccc ttgaggaggt ggaggcgtca aggttggtga ttcatcaaaa
     1321 tcatcgggag gttctggcgg cggcgctgtc aaatcatcca tccagggcgg caaatctggt
     1381 ggcggtggct caacggacgg actgggcacc atcggagtcg ccacggttgg cgccagcgcg
     1441 ggtcgagcgg tttgagccac cggcagccgg ttggtaatct ctggtgagag cgagggcggc
     1501 ggaaacgttg ggcgtgaacc tgctggtggt gcggttgagg cgccagaaaa ctttggcggg
     1561 gcaatggggg gagctgaagc accagaaaac tgtggcggcg ttggtgctcc cccggatggt
     1621 ccagaaaatt gaggccgggg cggaggcatt ggccgtgatg gggctggcgg cggattcccg
     1681 cgacttccag atggtgctgc tgggccacgc cctccacttc cagccggcgg tgggggtggt
     1741 gtggatggcg ccggagacgg cattccgcca gaaccaatca gcttttcgag ttgggccaga
     1801 agtgtttcaa ggcttttcag atgtgcaagt tgagttaact taaccagccc aaattctaac
     1861 tggaagcgtg gctcgggagc cgtgcgcagg gcttgttcga cttcggtgag gagcgtaaaa
     1921 aaccggacca ggtcttcgct ggaaaaggta cgggcatgct gctcgatctg gcggccttca
     1981 ctttcggaaa ccgggaggag ttccgggtca gcgcccgccg cttgaaacac cagcatatgc
     2041 cgcagatagg tcataacttc gtgcaaaaac tgccgcaggt catacccggt gcgcaccagt
     2101 tcatcaacca gtgtcagcat ttgtttgccg tcacgagcaa ccagggcatc aatgacctga
     2161 ccaacaaact gcggtccaat caggccgagc gaagccagta catctttttc ttcaatcgtg
     2221 gtgccggtaa aggcaatcac ctggtcaaac gccgattggg catcgcgcat actgccacga
     2281 ccggctgacg caatccggag cagggcatcc tgggaaatgg tgatgtgttc ggcttcgcca
     2341 atcagacgca ggcgttcggc aattttttca gtactgatgg ttcgaaattc aaactgctgg
     2401 caccgtgaaa ggatcgtttc gggcactttg tgcagttcgg tggtcgccat gatgaaaatt
     2461 acgtgcgatg gcggttcctc aagtgtcttg agcaaggcat taaacgcact gattgacagc
     2521 atgtgaactt catcaatgat gaagattttg tatcggtcgc gggcgggccg attggccacc
     2581 gtgctgatga tgacatcccg aatattgtca acgccggtgt gggaggcggc gtcaatttca
     2641 agcacgtcaa ttgagttccc gttggtgact tccagacagg atgaacactg gccacagggc
     2701 gtggcggttg gtccagtctg gcaattgagg gctttagcca gcaggcgggc cgtggtggtt
     2761 ttgcctacgc cacgtggacc ggcaaataaa aaagcatggt gcaatcggtt cccagtgatg
     2821 gcgttgcgga gcgtgcgggt gatggcttcc tgcccggtga cttcttcaaa tgcctggggc
     2881 cgccatttgc gggcaatgac ctgataggac ataaattctg cgcctcagcg tgaatggtcg
     2941 aggatgagtc gtagcgttac gttgaacacc gcaaaatact gcaactgggg atattttgaa
     3001 actgaatttg caactggggg aatcacgagg agggaaattt tgccccgaaa aagggccagg
     3061 ccctcaagtt accccacaca ctccataaga cttactaccg ttgctgtctt ccgaccctgg
     3121 cggagttcgc aagcttacag acgtgcagga cctgagggcc tgatacgtac tcactctatt
     3181 gagttatgag caagtcgcgc actatacgtc gaaaccgaag agcctgtcaa ctaaaaattt
     3241 ttcgttcttc agtcagtgtg cgcttaactt ttaagcaacc actttactct cagcgtttag
     3301 cccgagatta aaaactcatc aatcgggcac tgacgcggct gaaagcactg attgcgtcaa
     3361 aatattgagg attctcaaag agatttttcg cgatgttgag ctgtgaccag tcaatggtgg
     3421 ttggcgactc taacccaagg ctcaaccata gtccgcgaat gataaatgcc acaccaatca
     3481 cgccccaaat gccgataaat gccagtaaaa acagtgatgc aattgcgtta gccataaaaa
     3541 aaccttcctt ataattattc tttcaagttc gaagttggag gtgttgcgtt tcgagatata
     3601 gctatacacg tgcaactgat atgccagttc cataaaaatg aagtcaattt tacggatata
     3661 taacggattt tttcttttaa aatattctgt cataaggggt tattcgggga aaatagataa
     3721 gcccaatctg gatggtcaga tgggttcaat cagtgaaaag gacagttatt ttttcaagta
     3781 tggtgtggaa ttctttcaag agggggaaat ttttttacaa cagtatggtg aaaatccgtt
     3841 ctgagttgtc tgattaaaac cctgtctggt tagaaagtag gcgttttcct atcaaggtta
     3901 aaatagtttc tatcctggta ctgtgatgga gggttgaaaa aatctcggct atttttgaat
     3961 gagatttgat acactttgac ggtactgcat ccggtacctc ccctgacatc gcacgccatt
     4021 cacatcttca gcatcttcag tgttttttgt tggatgggga gttttcaaac cagactacat
     4081 tgcaatcgtg tgtgcgagga gcagcatgaa aattatcttc acgcatttga acggaagtcg
     4141 ggaaggccag gttgaagaat ataccttgcc cgtgatcaat gttggccgag gtgtggaaag
     4201 tgatattcga attggcgatg caacccccac ggatcgggtc gcttcacggc agcatgctga
     4261 attccggcgc gaagggaacc accttattct ctatgattta ggaagtcgaa atggtacgta
     4321 tgtcaatggt cagcgcgtga atggacagat tgaacttgcc acccatgatt tgatcgagtt
     4381 gggcattggc ggcccggcga tgcgagtcca ggtggttgtt tcagaaaccg aagaaattga
     4441 ttttctacaa aattcgcgct atttcggaag cctgagtcag gagcacctgt ccatggtgtt
     4501 gtcggctggc acgctggagg tgtatccggc tgacagctat ctgtttcgtg ttggtcaggc
     4561 gtgcgaatat ttgtatgtga ttaagtcagg ggttgtcgaa gtctggcgcg ataccaatga
     4621 aaatggtgag ctcgacctga caacctatct cgggagtggc gatgttttgg gcgcctcgtc
     4681 aattttactc gaaggggcca cgcatcattc agccgcccgg attccagaag gtgcggtgat
     4741 gttacggctc acccaatctg tgttccggac gctgatttta accattccag agtttgcgct
     4801 ggctgtgtgc acggcgctgg cacgccagct cgacagcacc tacaaaaaac tgcaaaccca
     4861 ttcatataaa cggttacaag gcaatctcaa cttctttgat ctggcgacgg tcattcaaac
     4921 actgattcag tctcgccaga caggtgtctt gagcgtgaca gcacttgaag tgggttttgc
     4981 gcattccggg ttgtgggcgt catctgatga tatcgaaatg ccggctgcca cgattcatct
     5041 ggtcaatggc aaggtggtgt atacccggtg tggtgttctc aacggggaag aagcgtttta
     5101 tcagcttttt cagtctgagt accatggcag ttttactttt caagaagggg cgccgcctga
     5161 ttcgggctca ccacagggaa cgatccatct tccaggattt aatctcctgc ttgaagccgt
     5221 acggatgcag gacgagttac gccagttcaa gctcaaacac ccggacccac acgtgaagta
     5281 tattccagcg actcagtcac tcacctggac ggaatctatg tatgacaaag ccgctcatgt
     5341 gatttgggag cggttaaagc gtggagcttc catcgccaac ctgcagggcg aggtgccata
     5401 ctgtgatttt gtaatttatt ttgtgattga tgtccttttg gagaaaggac ttctgagtgt
     5461 cagtggtgag ggcagccgat cataagacgg gtgattgctg ttcaggtgac ctggttttca
     5521 gcatcagaac ccgtgcactc catcaaaaca tagcgcacct attcgatttt gactgggcgc
     5581 tctgacaggc tgtgatgggt cggtctggga acaaccggat ctgcataccg ggctttgatt
     5641 tcaagaattt caggcaggac ctgttgaaag agttcaacca gctttggatc aaaatgtttt
     5701 ccgcgctcgc cttcgatata ggccatggtt tcctcaaccg accaggcttt tttatagggc
     5761 cgggctgaag tcaaggcatc aaacacgtcg cacaaggcca caatgcgggc aatgagtggg
     5821 atttcttcgc cttttaatcc tcgtggatac ccgctcccat cccatttttc atgatgacag
     5881 agggccagtt ccgaagccat ttgcattaac tctgaatcgt gtcc
//
LOCUS       DAOQDO010000058         5896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8260_length_5896_cov_2.844718, whole genome shotgun sequence.
ACCESSION   DAOQDO010000058 DAOQDO010000000
VERSION     DAOQDO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8260_length_5896_cov_2.844718"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PKZ53_01705"
     CDS             <1..884
                     /locus_tag="PKZ53_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNJ39175.1"
                     /translation="ATNSSLPEFFQASASDWKQAGLISSQIEALQASEELALKWQEEL
                     VRKKIQVIGQLDDNYPKRLNQVLTNQSPPILYCWGNWNLLQNPSIGFCGSRNASEQGI
                     NITKDTAKQVAQKGWTIVSGHAKGIDFTAHRTALENDGTTIIVAPEGILEFRLQEELK
                     KLAGSGKILILSEFQPNSHWSVANAMTRNHTICGLSDALVVVESRLSGGTFEAGKFAI
                     RVRVPLFVADYAEPEVNAPGNPYFIQNGAEPVRQNPQTHKANLHRLFTKVENHYINLE
                     KPIPKQKTLFAHEQFKL"
     gene            complement(949..1536)
                     /locus_tag="PKZ53_01710"
     CDS             complement(949..1536)
                     /locus_tag="PKZ53_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNJ39176.1"
                     /translation="MLAVDHQKKELHCKVVYYGPGLAGKVSNVVALYNLVRPEFRSKL
                     VTHKTDIASELTFHFPAGRFKTPKGYGLHFHLFSVPGAVWSPTNQKMILENVDGVIFV
                     ADSQPERIDADLDYLTMLKENLQALDIDFQTLPYVLQLNKRDIPGILSVEDLATRLRC
                     KNEPVFEAVAFKGIGVIETFKALARQILQKCQSNS"
     gene            complement(1594..2196)
                     /locus_tag="PKZ53_01715"
     CDS             complement(1594..2196)
                     /locus_tag="PKZ53_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNJ39177.1"
                     /translation="MKGHSMLVVDHQKKELHCKIVYYGPGLAGKTTNMRYLYDTLNSG
                     IRSEFVTHETDESRELTFSFPAGKFKALNDYVLYFHLYTVPGSFYVRQFRKQILENVD
                     GVIFVVDSHPRRLEAGVEFLNDLKENLQALKIDFQSLPFVLQLNKRDTPEAVSIEELT
                     TKLRCKNEPVIEAVADQGIGVVEAFRAIAQQFLQKHHTTH"
     gene            complement(2345..>5896)
                     /locus_tag="PKZ53_01720"
     CDS             complement(2345..>5896)
                     /locus_tag="PKZ53_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39178.1"
                     /translation="GNLFITDTENHAVYYADLARREVRLLAGEPGTPGKADGRGRAAR
                     FTRPRGGALSTDGRILAIADEGNNVVRLISRDGQVATLGRASSAKGSPIFEPHLGTQI
                     LKNPVFEPGTQFLKQAQPGDDITFNAPQSVSIDAAGNIYVVDQDGAFVVTRPNNQLPE
                     VVQLAQDGSFEQAASVVVRGTESFVLDTEATTEAEAVKVVTVGAPEIQNLSPNQVQLE
                     GGEEVTITGKNFAPESRIIIGDAEVTDFDVESATQIRVRVPKQNIPGNRNLTIQTRGG
                     VDQERILTVSKRFDELADGEITTVAGGIPYVGDGDLATGPTVGLRGNDLTLDVAGNIY
                     VSDYFNARVRRIDANTGVITTVAGNGRFTFVGDGGLAVSAGVGPTSATFDGEGNLYVV
                     DLINNRLRKIDPRTGIIQTIAGNGGFGFSPDGIPATQASLGIVYGATAVIDPQGNVVF
                     SDPSSNRVRRINSQTGIISTLTGNGTPGFSGDGGPATGAVLFAPHGIVFDQDGNLFIN
                     DAGNQRIRRIDARTGVITTIAGNGARGFSGDGGPATSASFNLERGGGMAFDPAGNLYI
                     ADGLNFRVRRIDRQSNIITTVAGSGNPAFGGDSGLATSAGMEPTGVAFDGSGNLLISD
                     FTNRIRRVDAQTRIITTIAGTGKTKFGGDGGLATNAGLSFLGGFSLDSQNNLYISDEP
                     FHYIHRVDGRTGIINIIAGTGNPGSHGDGGLATSAGLSSPRGQALDGQGNLYFADTFN
                     NRIRRINLQTGIITTVAGNGNESFGGDGGPATSAALFNPSGVAFDRNGNFYIADTGNH
                     RVRMVEASSGRISTVAGTGVRNFTGDGGPARSATLNLPQSVAFDPNGNLLICDAGSRV
                     IRRVNAQTGIISTIAGKPGDERFSGDGGLATQAGVAPAFVTSDAAGNLYIADYINHRI
                     RRIDAQTGIISTIAGTGREAYTGDGEPASTASLNNPYALVIDQAGNIFLADRENKAIR
                     VIKNGANYEPPPAGSPTLALIEDRTVRAGDLHVVEVRAFDPNGTGGLRLNLLESPDFV
                     ALIDNGNGVGELRIAPPATATQGGRVTVQVVDPTGLSAQTSFTVTVLGADEAEPTVSN
                     VTLSRKKVVRKKDATLGISWRSADDTGVMSHDLRFAADGSTFDTVVISGLDGSTQNFV
                     WTIPASVPKTKTGRVLIIARDAAGNTGVGTSQEITIK"
BASE COUNT         1461 a         1597 c         1420 g         1418 t
ORIGIN      
        1 cagcgaccaa ttcatcctta cctgaatttt tccaggcgtc tgcctctgac tggaaacaag
       61 caggactgat ctcaagtcaa attgaagcat tgcaagcatc tgaagagttg gccctgaaat
      121 ggcaggaaga actggtaagg aaaaaaatcc aggtcattgg gcaactagat gacaactatc
      181 ccaaacggct caatcaagtc ttgaccaatc aatcaccccc tattctctat tgttggggga
      241 attggaacct tttacaaaat ccatctattg gcttctgtgg ttcccgcaat gcatcagaac
      301 aaggtattaa cattacaaaa gatacagcga aacaggtagc acaaaaaggt tggacaattg
      361 tcagcggtca tgcgaaagga attgacttca ccgcacatcg cacggctctg gaaaatgatg
      421 ggacaacaat catagttgct ccagaaggca ttcttgagtt tcgtttacaa gaagaactta
      481 aaaagcttgc aggctcggga aaaattctca ttctctctga gtttcagcct aattcgcatt
      541 ggtcggtagc aaacgcaatg acccgcaatc atacaatttg tgggctaagt gacgcactgg
      601 ttgtcgttga atccagattg agtggcggca ccttcgaggc aggaaaattt gcaattcgtg
      661 tcagagtacc tttatttgtc gctgattatg cagagcctga agtcaatgct cccggtaacc
      721 cttactttat ccaaaacgga gcagaacccg tccgacagaa cccgcaaact cacaaagcaa
      781 atttacaccg gcttttcact aaagttgaaa atcactatat caacttagag aaaccaattc
      841 caaaacaaaa aacgctattt gcacatgagc aattcaaact gtgaatgcag actaattata
      901 tttattcaca atgatttaca atcagtttag aatcaagggc tgatactttc atgagttcga
      961 ttggcacttt tgaagtatct gccgggcgag ggctttaaac gtttcaatca cgccaatccc
     1021 tttgaacgcc actgcttcaa aaaccggctc gtttttgcat ctcaatctgg tagccaggtc
     1081 ttcaactgaa aggattccag ggatgtcgcg cttgttgagt tgcagcacat aaggcaatgt
     1141 ctggaaatca atgtcgagcg cctgcaggtt ttctttcaac atcgtgaggt aatccagatc
     1201 agcatctatt cgttcaggct ggctatcagc cacaaaaatc acgccatcaa cattttccag
     1261 gatcatcttt tgatttgtag gactccaaac agctcctgga actgaaaata aatggaaatg
     1321 aagaccatat ccctttggag ttttgaacct tcctgccggg aaatgaaagg tcagttcgct
     1381 ggcaatatct gttttatgag tgaccagttt gcttcgaaat tctggcctca ccaaattgta
     1441 cagagccacc acattggaca cttttccagc cagcccaggt ccgtaataga caaccttgca
     1501 atgcaattcc ttcttttggt gatcaacagc cagcataact gacctttcga aggttcactc
     1561 caggcattga aatcataaac cgtagctccc aggtcaatga gtcgtgtgat gtttctggag
     1621 aaactgctgg gcaatcgcgc gaaaggcttc gaccacacca attccctgat cagccacagc
     1681 ctcaatcact ggctcgtttt tgcacctgag ttttgtggta agttcttcga ttgaaaccgc
     1741 ttctggggtg tcacgcttgt tgagttgcag gacgaagggc aacgactgga aatcaatttt
     1801 gagcgcctgg aggttttctt tcaggtcatt gaggaactcg accccggctt caagtcgtcg
     1861 aggatgacta tccaccacaa agatcacgcc atcaacattt tccaggattt gttttcggaa
     1921 ctgccggacg taaaacgacc ctggcacagt gtagaggtgg aaatacaaaa cataatcatt
     1981 cagcgctttg aatttcccag ccggaaacga aaaggtcagc tcccgactct catcggtttc
     2041 gtgggtgaca aactcacttc gaatccctga gtttaaggtg tcataaagat accgcatgtt
     2101 ggtcgtcttc ccggccagcc cagggccgta atagacaatc ttgcaatgca gttccttctt
     2161 ttgatgatcc accaccagca tagagtgacc tttcatatat gtggggctga agactcgcaa
     2221 gctcggggct gaagatttcg ggctgaagac tttgggctca gggtattgaa cccattttct
     2281 tcagccctga gcttgcgaat cttcagcccg aagtcttcaa cccaaaatct tcagcccgct
     2341 gcctttactt gattgtgatt tcctggcttg tccccacccc ggtgttgcca gccgcatcgc
     2401 gggcaatgat taaaacacgc ccggtctttg tttttggaac tgacgcagga atggtccaga
     2461 caaagttttg agtgctgcca tcaagccccg aaataaccac cgtgtcaaaa gtcgaaccat
     2521 cggcggcaaa ccgcagatca tgcgacatca ctccggtgtc atccgccgaa cgccagctta
     2581 tgccaagcgt cgcatccttc ttgcggacca ctttcttccg cgaaagcgtc acgtttgaca
     2641 cagttggttc agcttcatca gcccccagca cggtcacggt aaacgacgtt tgggctgaaa
     2701 gcccggtcgg gtcaaccacc tgcaccgtca cccgcccacc ttgagtggca gtcgctggtg
     2761 gcgcaatccg tagttcccca accccatttc cattgtcaat cagtgcaaca aagtcaggtg
     2821 attcaagcaa gttcagtcgc aaaccaccgg tgccgtttgg atcaaaagct ctgacttcaa
     2881 ccacgtgcag gtctccggca cgcacagttc gatcttcgat caaggccagt gtcggggacc
     2941 cagccggcgg aggctcgtaa tttgcaccat ttttgatcac tcgaatggcc ttgttctccc
     3001 ggtccgctag aaatatgttc ccggcctgat caatcaccag tgcatacgga ttgttgagtg
     3061 aagcggtcga ggctggttcg ccatcgccgg tgtaggcttc gcggccagtt cctgcaattg
     3121 tggaaataat cccggtttgg gcgtcaatac gccggatgcg atggttaatg taatcagcaa
     3181 tatacagatt cccggcagca tcactggtca caaaggctgg tgcaactccg gcctgagttg
     3241 ccaggccacc atctccggaa aatcgttcgt caccaggttt tccggcaatg gtggaaataa
     3301 tccctgtttg ggcatttacc cggcgaatca cccggctccc ggcatcgcaa atcagcagat
     3361 tgccatttgg atcaaaggct acgctttgtg ggagattaag cgttgctgaa cgtgccgggc
     3421 caccatcacc ggtgaaattt cggacgccag ttccggccac ggttgaaatt cgaccagaac
     3481 tggcttccac catgcggaca cgatggttcc cagtatcggc aatgtagaaa ttcccgtttc
     3541 ggtcaaaagc cacgccagac ggattaaaca acgccgcact ggttgccggg ccgccatcac
     3601 cgccaaagct ttcatttcca tttccagcga cagtcgtgat gataccggtt tggaggttaa
     3661 tgcggcggat gcgattattg aacgtgtcag caaaatacag atttccctgc ccatcgaggg
     3721 cctgcccacg cggagaactc agccccgccg aggtcgccag gccaccatca ccgtgacttc
     3781 cgggattgcc ggttcccgca atgatattga taatgccggt tcgcccatct acgcggtgga
     3841 tgtaatgaaa cggttcatct gagatgtaga ggttgttttg ggaatcgagt gagaatccgc
     3901 ccagaaatga caatccagca ttggttgcca gtccgccatc gcctccgaac ttggtcttgc
     3961 cagtcccggc gatggtggtg atgattcggg tctgagcatc tacccgacga atgcggttgg
     4021 tgaaatcaga aatcaacagg ttgccggaac cgtcaaaggc caccccggtt ggttccatac
     4081 cggcactggt tgcgagacca ctatcaccgc caaaagccgg gttaccactt ccggcaactg
     4141 tcgtaataat gttggattgc cggtcaatgc gccgcacccg aaagttaagc ccgtcagcaa
     4201 tatacaaatt cccggctggg tcaaaagcca tgccgccgcc gcgctccagg ttgaaactgg
     4261 cactggttgc cgggcctcca tcaccactaa acccacgtgc gccgtttcca gcaatcgttg
     4321 taatcacgcc agtgcgggca tcaatgcgcc ggatgcgctg atttccagca tcgttaatga
     4381 agagatttcc gtcttgatca aacacaatgc cgtgcggagc aaacaacacg gctccggttg
     4441 cggggccgcc atctccgcta aaccctgggg ttccattgcc ggtcagggtt gaaataatgc
     4501 cggtttggga gttgattcgg cggacacgat ttgaagatgg gtccgaaaaa acgacattcc
     4561 cctgtgggtc aatcactgct gtcgcaccat acacaattcc aaggctggct tgcgttgccg
     4621 gaattccatc tggtgagaag ccaaagcctc cgttcccggc aatcgtctga ataattccag
     4681 ttcgggggtc aatcttgcgc agacgattgt tgatcagatc aaccacatac aaattccctt
     4741 ctccgtcaaa cgttgcagag gtaggtccaa cgccagcact caccgcgagg ccaccgtcac
     4801 caacaaaggt aaaccgaccg tttccagcta cggtggtaat caccccggta ttcgcatcaa
     4861 tccggcgcac acgagcattg aaatagtcag acacatagat attcccggcg acatccagtg
     4921 tcaggtcatt gccccgcaaa ccaaccgttg gaccggtggc caggtcgcca tctccaacat
     4981 aaggaattcc accagccacg gtcgtgattt cgccatctgc cagttcatca aaccgctttg
     5041 aaaccgtcaa aatccgttcc tgatccacgc caccacgagt ctgaatggtc aggttgcggt
     5101 ttccaggaat gttttgcttt ggcacgcgaa cccgaatctg ggttgcgctc tcaacatcaa
     5161 aatcagtgac ttcggcatcg ccaatgatga tgcgcgattc cggagcaaaa tttttgcccg
     5221 tgatcgtgac ttcttcgccg ccttcgagtt ggacctgatt gggtgacaaa ttctgaattt
     5281 ccggtgcgcc gaccgtgacc actttcacgg cttcggcttc ggtggtggct tccgtatcaa
     5341 ggacaaagct ttctgttccg cgtaccacca cactggctgc ctgctcgaag cttccatcct
     5401 gtgccagttg aacaacctct ggaagctgat tatttggccg tgtaacgaca aaggctccat
     5461 cctggtcaac gacataaata ttgccagccg catcaatgct caccgattgc ggtgcattaa
     5521 acgtgatgtc gtctccgggt tgagcctgct tcaagaactg ggttccaggt tcgaaaaccg
     5581 ggtttttgag aatttgagtg ccgagatgcg gttcaaaaat gggtgaacct ttggcactcg
     5641 aagccctgcc caacgtggcc acctgtccat cgcgggaaat cagccgcacc acgttgttgc
     5701 cttcatccgc aatcgccagg atgcggccat cggtactcag tgcgccacca cgcggacggg
     5761 taaaccgggc tgcgcggcca cgtccgtcgg cttttccagg cgttcctggc tcgccagcga
     5821 gcaggcggac ttcacgccgt gccagatcgg cataatacac agcgtggttt tcagtgtcgg
     5881 taatgaagag gtttcc
//
LOCUS       DAOQDO010000059         5892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8268_length_5892_cov_2.854549, whole genome shotgun sequence.
ACCESSION   DAOQDO010000059 DAOQDO010000000
VERSION     DAOQDO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8268_length_5892_cov_2.854549"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKZ53_01725"
     CDS             complement(<1..832)
                     /locus_tag="PKZ53_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39179.1"
                     /translation="MIILLLLMITLIPSDAVSQVPVAENEVSKADITVQLLPSKEKYS
                     LGGPIELTLIIQNTSQKDISLNILVPYNYPEGDYYPKERGLGFSPKNNLNDVSQRKKS
                     GEFAFPEVLSGRFVPLFLYSGDSSKENIYLNRYLNFQKPGRFEIKYSFKIHARREMKE
                     TSTLGVDLEFSNSFCINLESVTNQDLDQELEKFSEKLFLKSDFDQENFETFEGLCYLD
                     TPLSIKYIAKALDLERAEVVAAAILTRFNTPESLQYVEQALARFKDTKPGVIRSILFE
                     S"
     gene            complement(1154..2140)
                     /locus_tag="PKZ53_01730"
     CDS             complement(1154..2140)
                     /locus_tag="PKZ53_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39180.1"
                     /translation="MNTVDPNSRELVQYLLGELSESEQTAFEERFFEDDDLYEILRVT
                     EDELIDQYVTDHLPPAQRTLFEQRFLTHPHRRERIELARALHDKARSHRTRSLANPQT
                     GSWWQRWRGWFTFSGFPFTQLAFATAMVVLLVVSTLVVIDNRRMRRELDHMQSEQAAI
                     QQQTTTLNRQIAERTSQTERLTQELAQSRSLNVQLEQKLNTIKPAVVQRVTEAVISFV
                     LKAGTLRGEGPEQQIVIKNPKATVVLKLTLPSQMKATSFSARLATTEGKTTNSYVWST
                     TNLEAQTSGSGKYVTCVLSAQVLTPDTYILTLTSSSVDVDGEETDFQFSVSE"
     gene            complement(2233..2841)
                     /locus_tag="PKZ53_01735"
     CDS             complement(2233..2841)
                     /locus_tag="PKZ53_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ39181.1"
                     /translation="MPRSPTPITGQEAFARLLGLLGPDQVQAERNYDVLRQKLISFFI
                     NRNCLDPEEYADETFSRVLKRLLEGEIIRTDTPSKYFFSVAYNLIKEHWRRIEKSPES
                     LEDQIQLKQPAIDPYQLELEQERKSEHEAYLQCLDHCLETFPPETRELILEYYMEDGG
                     AGIERRKLLAERFGIQVNNLRIRMLRLREKLETCVRKCRETL"
     gene            complement(2892..>5892)
                     /locus_tag="PKZ53_01740"
     CDS             complement(2892..>5892)
                     /locus_tag="PKZ53_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ39182.1"
                     /translation="SGDSHRYTLRLKANHYIHLVVEQQGIDVAVQCFDPKGALLVEVD
                     SPNGTQGPEKLELVTSLAGDYQVTVKTFGDAVPPGKYLIELKDARLASAQETTRIRER
                     LLAQKIIAEAETLRLQHTEAAHRSALIKYESAISHCQAAGELEMEVETLNNLGRINAD
                     FGDNLKALDYYNRGLSLTKNLSDQTLKAGLYTNIGAVYWGQSEIQKAADAYSQALVLW
                     RELKNELQISVTLNNLSLCLIYFRQPKSAEPMLLEALKIQEALREVSSQATTLSNLGI
                     CSHYLSQKEKTHTYLEKALTVVNQLVPPNPMKKAELLHNLGILSSNAREIPQAFSYFK
                     EALTLFQTAGNRLRVAETLASLAVISYEQNKLTEAISYFNQVQQLIRSEDDQLWLPEM
                     YFTLSEIDQASGKLSEALAHLEKTIELVERRRQQVRSLDFRLNYFHDNEQYFQAYIAL
                     LMKLHAQNPAGNYDVLAFEGSERARARTLLDLLAEVEVDLWEGVDPKLLEQEHIVQAT
                     FNQKVNLWFGAKSDPNKSKVVKTLEAEIQDLNRNLNNLRAEMRAKNPRYAALTQAQTL
                     KLTEIQHKHLDPETLLLVYSLGASDSYVWAITTTSLKSYRLPGRTLIEATAQQVYQAL
                     SWTPEAQPARRSSPPSSLPLDGNPPDCIKPAQELSQMILGPLADQLGDKRLVVVADGI
                     LNLIPFGGLPSPLMSGTSDAQADQKALFLIEQHELTQLPSVSTLAALRQKAAPHNVVS
                     PTLFVVADPVFSATDSRVKKALAAKVSTGKMSTGKQPSKVATASSPPGLISQPDLPLE
                     LTSQLRDGQLARLSFTRLEASEISKVFSPSNVIQHLDFEASREMVFKENLSRYAYVHF
                     ATHGVFHSEQPALSGLILTLVDSNGQTQPGLLGLQDIYKLKLNAELVVLSACNSGLGK
                     LISGEGMMGLTRVFMTAGASRVVSSLWKVDDRMTSTLMKTFYTLMIQKKMSPAQALRE
                     AQLQLLRTPQWNSPRQWAAFQLQGEWR"
BASE COUNT         1418 a         1431 c         1420 g         1623 t
ORIGIN      
        1 cagattcaaa caagatagat cgaattactc ccggctttgt atctttgaac ctggcgagtg
       61 cctgctcaac atattgaagt gattctggtg tattgaatcg ggtcagaatt gcagcggcaa
      121 caacctcagc tctctcaaga tccagggctt tggctatata ttttattgac agaggggtat
      181 ccaaataaca tagtccttcg aatgtctcaa aattctcctg gtcgaagtca cttttcaaga
      241 agagtttctc tgaaaacttc tccagttcct gatcgaggtc ttgattcgtt accgattcaa
      301 gattgataca gaacgaattg gaaaactcta aatcaacccc cagtgtcgag gtttctttca
      361 tctcccttct cgcatggatt ttgaatgaat acttgatctc aaatcttcca ggcttttgaa
      421 aatttaaata tctatttaaa tagatatttt cttttgatga atcacccgaa tataaaaaca
      481 aaggaacaaa acgaccactt agcacttccg gaaaagcaaa ttcaccactt tttttccttt
      541 gacttacatc attcagatta ttcttagggc taaagccaag tccccgttct ttcgggtaat
      601 aatccccttc tggatagttg tagggaacca gaatattcaa tgaaatgtct ttttgagatg
      661 tattttgaat aatcagggtt aattcaattg gtcccccaag agaatatttt tcttttgatg
      721 gtaacagttg gacagtgata tccgctttcg atacctcatt ttcggccaca ggtacttgag
      781 acactgcatc agaaggaatc agcgtaatca tcaaaagcaa taaaataatc ataagtacgc
      841 accttatcgt cagttcccag agatcttgaa tttggcaagt gcttcatttt tctgtatttc
      901 cgtggttctc gtagctaaaa ggttgagaaa cttccggtaa gctccggcta cccagccgtc
      961 ttttttcaat actacaacct gtctccactg ccatccttcc agtttgcgga agctgtcttt
     1021 gttttatacc ctttattcta attcgcttgc gggattacac taatgaggca atgccccact
     1081 ccctttcaaa gaaacactct tggtgagatt ttagaccagg cttgagccaa ttcagggaga
     1141 caccagcgga accctattca ctcacgctaa actgaaaatc agtttcttca ccgtctacgt
     1201 caaccgaact gctggtcagg gtcaaaatat atgtatctgg ggtgagcacc tgggctgaaa
     1261 gcacgcaagt cacatacttc cctgagcctg aagtttgggc ttccaggttt gtggttgacc
     1321 agacataaga gttagtcgtt ttgccctctg tcgtggccag ccgggccgaa aatgacgtgg
     1381 ctttcatttg cgacgggagc gttaacttta acaccacggt ggctttaggg tttttgatca
     1441 cgatttgttg ttctggccct tcaccccgca gcgtcccggc tttcaacaca aacgaaataa
     1501 ccgcttctgt cacgcgttga accacggctg gtttgatggt attcaacttc tgttctaact
     1561 ggacattcaa tgatcgtgac tgggccagtt cctgggtcag tcgctctgtc tgacttgtcc
     1621 gctctgcaat ttggcggttt aaggttgtgg tttgctgctg gatggcggcc tgttcagact
     1681 gcatatgatc cagttcccgg cgcattcggc gattatcaat caccaccagc gttgaaacga
     1741 ccagcaaaac caccatcgcc gtagcaaaag caagctgggt gaatggaaat ccagaaaagg
     1801 taaaccagcc acgccagcgc tgccaccagg aacccgtttg gggattggcc agacttctgg
     1861 ttcggtgcga ccgggcttta tcatggagtg ctctggcaag ctcaatccgt tcccgccggt
     1921 gtgggtgggt aagaaaccgt tgttcaaaca gggtgcgttg agcgggcggc agatggtcgg
     1981 tcacatattg atcaatcaat tcgtcttccg tcacacgcag gatttcatag aggtcgtcat
     2041 cctcaaagaa tcgttcctca aatgcagttt gttcagattc agagagttcc ccaagcaggt
     2101 attggaccag ctccctggag ttggggtcta cagtgttcat gatgttatgc ctcagtgatt
     2161 gagtaactgg atgtgacgaa tacgcgttag ggggaggact gtgcacttag ttgtgtcctg
     2221 aaaggaattt tattacagcg tttcccggca ttttcgcaca catgtttcca gtttttcacg
     2281 caaacgcagc atccggatac gcaaattatt gacctgaata ccaaatcgct cagccagcag
     2341 tttcctccgt tcgatcccgg cgccgccatc ttccatatag tactcaagaa tcagttcccg
     2401 cgtttctggt ggaaaagtct caagacagtg atccagacat tgtaaatagg cttcgtgttc
     2461 agattttcgt tcctgctcaa gttcaagttg atagggatca atggctggct gtttgagttg
     2521 gatttgatct tcgagtgatt cgggtgattt ttcgatgcgc cgccaatgtt ctttgatcag
     2581 attatacgcc acgctgaaga aatatttgga tggggtgtca gtccggataa tttcaccttc
     2641 cagcaaccgt ttcagcaccc gactaaaggt ctcgtccgca tactcttccg ggtctaagca
     2701 gttccgattg ataaaaaatg aaatcagttt ttgccgcagc acatcataat tgcgctcagc
     2761 ctgaacctgg tctggtccaa gcagaccgag caggcgggca aacgcctcct ggcctgtgat
     2821 gggggtcgga gatcgcggca aactctcttt ttccatgtgg cctcagattt gttgagcgga
     2881 agattgtcgc attagcgcca ctcaccttgc agttgaaaag ctgcccactg gcggggtgaa
     2941 ttccattgcg gtgttcggag caactgtaac tgggcctccc gcagtgcctg ggctggagac
     3001 atcttttttt ggatcatcag cgtgtagaag gtcttcatca acgttgacgt catccggtca
     3061 tcaactttcc agaggcttga aactacccgg ctggcacctg ccgtcataaa aaccctggtt
     3121 aaccccatca taccttcacc actgatcaat tttccaagac cgctgttgca cgcacttaac
     3181 accaccagtt cagcattgag tttcaattta taaatatctt gcaaacctaa taatccaggc
     3241 tgagtctgcc cgtttgaatc aaccagcgtc aaaatcaatc ctgaaagcgc tggctgctca
     3301 gagtgaaaaa caccatgggt cgcaaaatgt acataggcat accggcttaa gttttccttg
     3361 aacaccatct ctcgactggc ttcgaaatcc aggtgctgga tcacattgga tggtgaaaac
     3421 actttggaaa tttctgaggc ttccagccga gtaaaggaaa gtcgcgccag ttgaccatca
     3481 cgcagttggg aggtcagttc cagtggcaga tcaggttggg aaatcagccc aggggggctg
     3541 gaagccgtcg ctacttttga cggctgctta ccggttgaca ttttgccagt tgatactttg
     3601 gcggccagtg ctttttttac acggctgtca gtggcggaaa ataccgggtc cgccaccaca
     3661 aataacgtgg gtgataccac attgtgtggg gctgctttct ggcgaagtgc cgccagagtc
     3721 gagaccgagg gcaactgagt gagttcgtgc tgctcgatga gaaacaacgc cttttgatca
     3781 gcttgagcat ctgaggttcc agacatcagc ggcgacggga gccctccaaa aggaatcagg
     3841 tttaagatcc catcggcaac cacaaccaat cgtttgtcgc caagctggtc agccaggggg
     3901 ccaaggatca tctggctcaa ttcctgggct ggtttgatac aatctggcgg attgccgtcg
     3961 agaggaaggc tggatggagg tgaagaacgg cgggcaggtt gggcttccgg agtccaggat
     4021 aacgcctggt aaacctgttg cgcggtagcc tcaatcagtg ttcgaccagg aagccgataa
     4081 cttttcaaac tggtggtggt aatggcccac acataactat cagacgcccc aaggctataa
     4141 accagcagga gggtctctgg atccaggtgt ttgtgttgga tctcggtcaa tttcaaagtt
     4201 tgagcctggg taagtgccgc atacctgggg tttttcgcgc gcatttcagc tctaagattg
     4261 ttgaggttac gattcaaatc ctgaatttca gcctccaggg ttttgaccac tttcgactta
     4321 ttgggatctg atttggcccc aaaccagaga tttacttttt ggttaaaggt cgcttgaacg
     4381 atgtgttcct gttcgagcag ttttgggtca acaccttccc aaagatccac ctctacttcc
     4441 gcaagcaaat cgagcagcgt ccgggcgcgg gcgcgttcac ttccttcaaa tgccagaaca
     4501 tcatagtttc ccgctggatt ctgggcgtgc aatttcatca agagtgcgat gtaggcttga
     4561 aaatactgct cattgtcgtg gaaatagttg aggcggaaat ccagactgcg gacttgctga
     4621 cgtctccgct caaccagttc aatagttttt tccaggtgag cgagcgcttc agagagcttc
     4681 cctgaagcct gatcaatctc agacaaggta aagtacattt caggaagcca taattgatcg
     4741 tcttcagagc gaatcaattg ctggacctga ttaaagtagc tgatagcctc tgttaatttg
     4801 ttttgttcgt atgagatcac tgccagcgaa gccaatgttt ccgcgacacg caaccgattt
     4861 ccagccgttt gaaacagggt caaggcttct ttgaaatacg aaaacgcctg tggtatctca
     4921 cgggcattac tgctgaggat cccaaggtta tgtagaagtt ccgccttttt cattgggttt
     4981 ggtggaacca gttgattgac cactgtcaga gccttttcca gataggtgtg agtcttctct
     5041 ttctggctga ggtaatggga acatatcccc aaattgctca atgtggtcgc ctgtgaagaa
     5101 acctctcgca gggcttcctg aatcttcaag gcttctagca gcatcggttc cgcacttttc
     5161 ggctgccgga aataaatcag gcacaggctc aggttattga gtgtcaccga aatctgcaac
     5221 tcatttttca gttcgcgcca gagcaccagc gcctgggaat aggcatcggc ggctttttga
     5281 atttccgatt ggccccagta cacagcccca atattggtgt acaaaccagc cttcaacgtc
     5341 tggtcagaca ggtttttggt gagggacagt ccccggttgt aatagtccag cgctttcaga
     5401 ttgtccccaa aatcagcgtt tatccggcca agattgttga gcgtttcaac ttccatttcc
     5461 agttcaccag ccgcctgaca gtgggaaatc gctgattcat atttgatcaa ggctgaccga
     5521 tgtgctgctt cggtgtgttg aagacgtaag gtttcggctt cggcaatgat tttttgggcc
     5581 agcaggcgtt cgcggatgcg cgtggtttcc tgggctgacg ccagccgggc gtctttgagt
     5641 tcaatcaggt atttccccgg tggaacggca tcgccaaagg ttttcaccgt cacctgataa
     5701 tctccggcca gggaagtaac cagctccaat ttctctggcc cctgggttcc atttgggcta
     5761 tccacttcaa ccaggagcgc tccttttgga tcaaaacact gcacggcaac atcaatgccc
     5821 tgttgttcaa ccaccagatg gatatagtga tttgccttga gcctgagtgt gtacctgtgg
     5881 ctgtcaccac tt
//
LOCUS       DAOQDO010000060         5866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8331_length_5866_cov_2.830150, whole genome shotgun sequence.
ACCESSION   DAOQDO010000060 DAOQDO010000000
VERSION     DAOQDO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_5866_cov_2.830150"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKZ53_01745"
     CDS             <1..477
                     /locus_tag="PKZ53_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ39183.1"
                     /translation="IRKKVLDYTTEEILSLFNPTLFSALELPREPHPRLKQSNQASVI
                     LIGSIAGMTALPTGIPYGATKAAMAQMARGLAFEWAADGIRVNAIAPGFIQTPLTHPV
                     LANPQFMAHFQSRVPLKRPGTPEEISSLAAFLALPAASYITGQTIVADGGLTIQSL"
     gene            931..1524
                     /locus_tag="PKZ53_01750"
     CDS             931..1524
                     /locus_tag="PKZ53_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ39184.1"
                     /translation="MPNIALLPQPHSSGPSGTLETLFREHSEMVVQAAYRITGNSVDA
                     EDVLQTVFLRLASREEKLDLSPNPKSYLYRAAINAALDIVRSRSSLSSLTLEDQVADM
                     AADQRHNPEAQQADAELRRVVQASVAQLGRSAAELFVLRFFEGYTNEEIADLLGMSKL
                     VVAVLVHRARTRVKKELKQYLETHHDTEKRHRGIIVR"
     gene            1484..4117
                     /locus_tag="PKZ53_01755"
     CDS             1484..4117
                     /locus_tag="PKZ53_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNJ39185.1"
                     /translation="MTPKKDTVESLFDKSTSHIQGQQMTSAEVEQATQRVWARLGTDT
                     SIAEAELTSVDQIRTCSDFQSLIPAFLAGRLTSARSLLVEDHTRECVPCRKALKEAKN
                     GTQRKAQPAGIGAVAGPAIWKRYSIAASFLIGFALLGLVIAQFFLTSVDAGTATVVSV
                     DGDLYRIGDTENRRLAVGERVTLGEIVRTGNHSRAQVRLSNNAVVELDERTEFSLRQA
                     TQGSTIQLERGSIILQAPTGVKGALFVSTDDSLISGKDMILAVNHGTKGSRVSCIQGT
                     GTVSNAGKDQPLQAGQQFTSSTSVEKLPIQEEISWSKDADRYAKLLEGLQHLYQDLDQ
                     TVPRPGLRTTPRLLPMLPDNTVFYLALPNIGESLVASQQIVEKHLQQNPELAAWWKSE
                     KSATATPQYNQILAEIRTVSGFLGNEIVITAAGGTQIKSEPVVLAEVKDEAGLRNYLT
                     SHLAGTTGKMAFQLISDAELKTPVTAAGEQAILGWIQNGTLVLATSRDGITQAVNRMQ
                     SSKSAPAGSLLSAIEAEYKNGVELMLAIDLSQMNDGAEAKAKADGSKAASEFSNFKHL
                     IVNLKEVNGETSNRAVVTFKQPKKGIATWISSPGPMGSLSFISPDASLVSAFVVENPA
                     GMLTELFDTLQQEDPTFLSELQALEAKHGLRIREDFAAYFGGEVAFAVDGPMLPTPAW
                     KLVMEVNDPVKLQASFAKAIVEVNSLIKPEENLTFSLDSTTVDGQVYHTVRKNGTAEV
                     TTYTFAHGYMVAAPTQSLVSQALRYYESGISLVSAPRFLSALPKDGNTNFSMLMYQNL
                     TPMLTQFADSSNEKKPEGLKLGAMAAAALPTLAYAYVNDDQMIFALGGDGIIKLSPTN
                     LLTIPGSIGLQALKGHPEKAK"
     gene            4676..5383
                     /locus_tag="PKZ53_01760"
     CDS             4676..5383
                     /locus_tag="PKZ53_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNJ39186.1"
                     /translation="MRTTKSISRIIGILLLVQLAGFIVPFVILQPIIMAGFLTSAAGN
                     AFQIKTAVFLLFWNCAVTIAISIAGWPIFRQSSEAASLLLVAVSVMMFVLQAVDNIQL
                     LSMLSLSQQYTQAGGQEELFQTLAAAIRSTRKWAHYSELLMIDAWICVFYSLLYRSVL
                     VPRAVALLGLTTITLHFTGMTMPLFLGYRGVAVMAMSIILSHLTLAVWLVCKGFDDSS
                     LLPGVKAHSPAISQSFS"
     gene            5410..>5866
                     /locus_tag="PKZ53_01765"
     CDS             5410..>5866
                     /locus_tag="PKZ53_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNJ39187.1"
                     /translation="MNHQYSTISPQWYARSCGILYLYIFVAGIFAEVFVRSKLIVPKD
                     AATTATNILANESLFRLGFSGELLHLSCDVAVAVLLYALFRRVNREVALLAALMRLMC
                     ALVLAVASLGHFAALQLLGGLNYLKTFTPDQLQSLALLAMKLQGDGYTIA"
BASE COUNT         1406 a         1554 c         1528 g         1378 t
ORIGIN      
        1 atccgcaaaa aagtgcttga ttacaccacg gaagaaatcc tgagtctctt taaccccacc
       61 cttttctcgg ctctggagtt gccccgcgaa ccccaccccc gcctcaaaca gagcaatcag
      121 gcgagcgtga tcttgattgg ttcgattgcc ggaatgaccg ccttgccgac cggaattccc
      181 tatggcgcga ccaaagctgc catggcccag atggcacgag ggttagcgtt tgaatgggcg
      241 gcggatggca ttcgggtcaa cgcgattgcg ccgggattta tccagacgcc gttgactcat
      301 ccagtcctgg ccaatcctca atttatggcg cacttccaaa gccgggtccc gcttaaacgt
      361 cctggaactc cggaagaaat cagttcactg gcggcgtttc tggccttacc cgccgcgagc
      421 tatatcaccg ggcaaacgat tgtggctgat ggcggattga cgattcagtc gctataaatc
      481 gaggacgaca ggagttggga atctggctcc aaattgtgta gccaccagtt aaaccgaggt
      541 gtagccacca cttgaaagcc actggctagc cacattatcc aatcatgtgg ctagccacta
      601 gcgaaactaa gcatggctta agttaatgaa atcagtagtt tacagttggt tcgttgagag
      661 tattcatatc agtcaaaaaa taccagacac taaattgtgt agccaccagt taaaccaata
      721 aaactgaggt gtagccacca gttgcaagta ctggcgagcc acctgcttca atcaggtggc
      781 tcgcggctga ctctggacca gtgaaactgg tacttttcaa gcgatctttc tctgtcacca
      841 aatccaaaat ccgaaaaatc ttttttgacg gttgttaata aattggcgca tggcccgttt
      901 tacaggtaag aaccaaaact ggagggaccc gtgccaaaca ttgcgctatt gccacagcca
      961 cactcgtcag gaccttccgg gacgctcgaa acactgtttc gcgagcattc cgaaatggtt
     1021 gtgcaggcgg cgtaccgcat caccggcaat tcggttgatg ctgaagacgt cctgcagacg
     1081 gtttttctgc ggctggcaag ccgcgaagaa aaactggacc tgtcacccaa ccccaaatcc
     1141 tacctctacc gcgccgccat caatgcggca ctcgatatcg tccgcagccg aagcagcctg
     1201 tcttcgctga cactggaaga ccaggtggcg gatatggccg ccgatcaacg tcacaacccc
     1261 gaagcacagc aagctgatgc cgaacttcgt cgcgtggtgc aggcatcagt tgcacaactg
     1321 gggcggtctg ccgccgaact gtttgtgctg cgctttttcg aagggtacac caacgaagaa
     1381 attgccgact tgcttggaat gtccaaactt gtcgttgccg ttttggtaca ccgggcacgc
     1441 acccgcgtca aaaaagaact aaaacaatat ttggagacac accatgacac cgaaaaaaga
     1501 caccgtggaa tcattgttcg ataagtccac ttcacacatc caggggcagc aaatgaccag
     1561 tgccgaggtt gaacaggcta cccagcgggt gtgggcgcga cttggaactg acacgtcaat
     1621 tgccgaggca gaattgacct cagttgatca aatccgtacc tgctctgact ttcagagtct
     1681 gattccggcg tttctggccg gacgcctgac ctcggctcgc tccctgctgg tcgaagacca
     1741 cactcgggaa tgtgttccct gccgcaaggc actcaaagaa gccaaaaatg gcacccagcg
     1801 caaggctcag ccggcgggca ttggcgccgt tgctggacct gcgatctgga aacgatactc
     1861 aattgccgct tcgtttctga ttgggtttgc cttgctgggt ctggtgattg cccagttttt
     1921 cctgacctcg gttgatgcgg gtaccgccac cgtggtttca gttgatggcg atctgtatcg
     1981 aatcggcgac actgagaacc ggcgtctggc ggttggcgaa cgagttacgc tcggcgagat
     2041 tgtccgcacc ggcaatcact cacgggccca ggttcgcctg tcaaacaacg ctgtggttga
     2101 actcgacgaa cgaaccgagt tttcgcttcg ccaggcgacc cagggctcaa ctattcaact
     2161 ggaacgcggg tcaattattt tgcaagcccc gaccggcgtg aaaggtgcgc tctttgtctc
     2221 aaccgatgac agcctgattt ccggcaaaga tatgattctg gcggtcaatc acggcaccaa
     2281 aggttcgcga gtgtcgtgta ttcaaggcac cggcacggtg agcaatgccg ggaaagacca
     2341 gccattgcag gccggtcaac agtttacgag cagcacctcg gttgaaaaac tcccgattca
     2401 ggaagaaatc tcctggagca aagatgctga ccggtatgcc aaattgcttg aagggttgca
     2461 gcatctgtat caggaccttg atcagacggt gccgcgtcct ggactgcgca ccacaccgcg
     2521 gttgctgccg atgctgccag ataacacggt gttttacctg gccttaccca atattggcga
     2581 gtcgctggtt gcctcacagc agattgttga aaaacacctg cagcaaaacc cggaactggc
     2641 ggcctggtgg aagtctgaaa aatcggcaac tgccacacct caatataacc agattctggc
     2701 tgaaatccgc actgtctcag gatttttggg aaatgaaatc gtgattaccg cggctggcgg
     2761 aacccagatc aaatcagaac cggtggtgtt ggccgaagtc aaagatgaag ctgggttgcg
     2821 caactatctg acctcacatc tggctggaac aaccggaaaa atggcgtttc aactcatctc
     2881 agacgccgaa ttgaaaacac ccgtcactgc ggctggcgag caggcgatac tgggctggat
     2941 ccaaaacgga accctggtgc tggccaccag ccgtgatggg atcacccagg cggtgaaccg
     3001 gatgcagtct tccaaatcgg caccagccgg gtcgctgctc tcagccatcg aagccgaata
     3061 taaaaatggc gttgaattga tgctcgccat tgacctgagc cagatgaacg acggtgctga
     3121 ggccaaagcc aaagcggatg gctcaaaggc agcttctgaa ttcagcaact tcaagcacct
     3181 gatcgtcaac ctgaaggaag tcaacggcga aaccagcaac cgggcagtgg tgacttttaa
     3241 acagcccaag aaaggtatcg cgacctggat ttcaagccct ggcccaatgg ggagcctcag
     3301 ttttatctca ccggatgcca gcctggtttc agctttcgtg gttgaaaacc cagccgggat
     3361 gctgaccgaa ttgtttgaca cgctgcagca ggaagacccg acgttcctgt ccgaacttca
     3421 ggccctcgaa gccaaacatg gcctgcgcat ccgtgaagat tttgctgcct actttggcgg
     3481 tgaagtggcc tttgccgtgg atggcccgat gctcccaacc ccagcctgga aactggtgat
     3541 ggaagtcaac gacccggtca aactgcaagc aagctttgcc aaagcaattg tggaagtcaa
     3601 ttcactgatc aaaccggaag aaaacctgac cttcagtctc gattcgacga cggtggacgg
     3661 tcaggtgtac cacaccgtgc gcaaaaacgg cactgccgag gtcacaacct acaccttcgc
     3721 tcacggatat atggtcgccg ctccgactca gtcactggtt tcacaggcac tgcgctacta
     3781 tgaatcagga atttcactgg tgagtgcccc gcgctttctc tcagcgcttc ccaaagatgg
     3841 caacaccaat ttctcgatgc tgatgtacca gaacctgacg ccaatgctca ctcagtttgc
     3901 cgactcatcc aatgagaaga aacccgaagg tctgaaactg ggtgcgatgg ctgctgccgc
     3961 cttgccgacc ctggcttatg cctacgtcaa tgatgaccag atgatctttg ccctcggcgg
     4021 tgacggcatc atcaaactca gcccaaccaa tctgttgacc attccgggtt caatcggttt
     4081 gcaggcgctg aaaggccatc ctgaaaaagc caaatgaaga atgaagaatg aagaatgaag
     4141 aatgaagagt gaagagtgaa gaatgaagaa tgaaggattg aactgcttcg ttcctaaatt
     4201 ctaaattctt cactctaaat tcttcattct tcattctttg tggactacaa accaaaaact
     4261 gaacctcgca gggaagagtc catgacgtca cttcacaaac aggccagagg tgcaggagct
     4321 atctatttgg tacttgtaaa ggaaatgcaa ccgcgacggc cagcgacatt gccgctcgcg
     4381 agttgctttt tcgttttggg atggttggtg atttgtgctg tggcgttatc ttctccgttg
     4441 cggaaaggtc ggattgagta tcctgaatgg cagcgcttcg cagtgcaaac ccagcgcttt
     4501 ttccaaacag caacgtcgct tgtcaacttt gtttgacctt tccgccacgg agttctaatg
     4561 aaggtctagg cgccaaaata cacacagcta accgcttttg agacaggaag accgggaaac
     4621 aggcacaaga accggagcgc ttatccgctg gtttggagat attttggagg taaaaatgag
     4681 aaccaccaaa agcataagcc gaataattgg tatcctgttg ctggtacagt tggctggatt
     4741 tattgtgccg ttcgtaatat tgcagccaat tataatggcc ggtttcctta ccagtgccgc
     4801 cgggaacgcc tttcagatca agacagccgt cttcctgttg ttttggaact gtgcggtgac
     4861 aattgccatc tccatcgccg gctggccaat ctttcgtcaa tccagtgagg cggcctcgct
     4921 tcttctggtc gcggtgagcg tgatgatgtt tgtgctgcaa gccgttgata acatacagtt
     4981 gctgtctatg ctgtctttga gccagcaata tacccaggca ggtggtcagg aggagctttt
     5041 tcaaacgctt gccgctgcta ttcgctcgac aaggaagtgg gctcattact cggagttgct
     5101 catgattgat gcctggatct gtgtgtttta cagcctgttg tatcgttcgg tcctggtgcc
     5161 gcgtgcggtg gctcttttgg gattgacgac gatcacgttg cattttaccg ggatgaccat
     5221 gccgctgttt ctggggtatc gcggtgtggc ggtgatggcg atgtcaatca ttttgagcca
     5281 cctcactctg gcggtatggc tggtttgcaa aggctttgat gattcctccc tcttgccggg
     5341 tgtcaaagcc cattcacccg caatcagtca gtctttttcc tgaaatcgaa ccaaaacaaa
     5401 agaatcgcta tgaaccacca atattccacc atatcacctc aatggtatgc ccgcagttgc
     5461 ggcatcctgt atctgtacat ttttgtggca ggtatttttg ctgaagtttt tgtcagatcg
     5521 aaattaatcg tgccaaagga tgcggcgacg actgccacca atatcctggc caatgaatcg
     5581 ctgtttcggc tcggtttttc aggtgagctg ttacaccttt cgtgtgatgt ggccgtggct
     5641 gtactgctgt atgctttgtt cagacgcgta aatcgggaag ttgctctgct ggcggcattg
     5701 atgcggttga tgtgtgctct cgtcctggcc gttgccagtc ttggccattt tgcagcgttg
     5761 caactgttgg gaggtctcaa ctacctgaaa acgttcacac ccgaccagtt acagagcctg
     5821 gctttgctgg ctatgaagct ccagggagat gggtatacca tcgccc
//
LOCUS       DAOQDO010000061         5863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8335_length_5863_cov_2.934229, whole genome shotgun sequence.
ACCESSION   DAOQDO010000061 DAOQDO010000000
VERSION     DAOQDO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8335_length_5863_cov_2.934229"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PKZ53_01770"
     CDS             <1..1337
                     /locus_tag="PKZ53_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ39188.1"
                     /translation="RKIFHSSPVAIALLSLTDSKVLDVNPAFLQMCGYTRGEVVGRPP
                     LELNLWINLSDRERLLQAISSGKTPFLIETPFRSKSGEFRQASGAVELIHLDEGPCIL
                     AMFYDVTERQLREGQMQLLESAVLNSLDAILILEPHPDNPRDIRIVFVNEAFTRLTGY
                     SREEALGQSLAIMHGPKTDLNEVARIRAIRLAGKPFSSELIHYRKDGTPFWCEASCIP
                     VFEPEQNLRRWVLFQREITERKEYEAKLQELNQHLIDTLERISDGFVAFNSEMEITYA
                     NKVAENLTGVPLEKLLGRNLLIYFPQATNNFFYTNYLTALREQRPVHFEAFSEVFRGW
                     YEVHAYPSANGLSVFFRDVSERYRLEAEYRKTAHLESLADQVGEVAQEFEQVLTRMQA
                     SVRHVQELTEGLPKVTKPMELLVESSHQSSHLAGRLLVLSQERTLPEAKEES"
     gene            1564..2715
                     /locus_tag="PKZ53_01775"
     CDS             1564..2715
                     /locus_tag="PKZ53_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39189.1"
                     /translation="MTFLVNHRFDRVTKKGLVFAVVVLCLLICHSATHRFSLEDTYFP
                     AQSKAYDGPCSIDITGAGQVKKAAAPKTHPDSGIELVLRQDKACFYQGGNVWLEMKMT
                     NTSKQSVAIEPCLNRFFTLPTQEMHFDVRNERHEPVPQLLTAFPSTIRWSKHRSTPKK
                     AIKPSENYSQSFCLNVLFDLTQPGLYSVELEQLVHCEDGTPVHLTSNQVWFQIFEDQT
                     ISEFGEPVSAIGLVTSQGMTSRGEITCLLAYRLHLGLQKQVFEIWDPINVDLAFLNPT
                     KKVIVLEDFHILFMCLEFEILRENGQPVSKTQLCRYLEKGPAFFHDESNRLPPLASVF
                     YRLPLKKLFEINERGTYVMTVRMKLPNNRVATSNQIRFTVQGSWLPKWF"
     gene            2726..3757
                     /locus_tag="PKZ53_01780"
     CDS             2726..3757
                     /locus_tag="PKZ53_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39190.1"
                     /translation="MPNHTSTRTFKIVQEHSPRRCEVCHQADQFDPETSHCLRCQTVF
                     KPVLTATQRQSSGLLRLQNRPQQLTFSSVTFIVKSALSRGSIRSFAQVFGVVVGVELP
                     LAGLAWMLGRWGSPWLWPLYLIAAPVSMWGAECVISRLVADSILGNQSSIFEAVRTIW
                     NRRIAFLSDVLDDAFDGPFYRWSMVVVGVTIGALVGEVFRREMAFGVMFGLYLWIGVS
                     VCTQRAFSRSFLVNQVSILDQEVESDVWHKSSQLGEQRMFKLWNAALWFVRIGLASLV
                     VAPSLSQVLSLSSPLIWYPMLPVYLAEQQVVCFLILVFLKLLILPTWLALKVHLYFVL
                     REQSPVASK"
     gene            3783..4889
                     /locus_tag="PKZ53_01785"
     CDS             3783..4889
                     /locus_tag="PKZ53_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39191.1"
                     /translation="MLRLCKFRQFVSLVVVILMLCFGLEPGIQAADEPPSQRQSRYMD
                     EGERLYRAFTDGCHVALGVLTEVGPRCEDCPESSDKEVLNRRVKIYLTEWIWGKPAET
                     MTELHTTQVTEIGGHKSSYGAWSAWEGVTVEEGHELLIVWWEKKALESQLLWKPGPEV
                     LVTDNQSKINQIRKIADLFAKYKAKPGEFASLFQQALDVNSSPLDAMTLGFAFALIQH
                     TGWKQGDEGARWLVWLLNMNFKQLDPQAYLWGLFYQLTQIFGMLSTTSPQMREVVLQQ
                     IIQTACSGDLVKAGRAIACLRKLCEFNLLKETPGLSQRLSSDQKRLLSERYQSLVSQK
                     KFPRSLSFENQCGLISFVPFDSESSSQKESNDGE"
     gene            complement(4862..>5863)
                     /locus_tag="PKZ53_01790"
     CDS             complement(4862..>5863)
                     /locus_tag="PKZ53_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39192.1"
                     /translation="EKLVQEADVIVVGQVLTIDQLEETTYLENGFNIEGYRHHARFHI
                     DRVIHTPSELQMKDRLEFDYFRSSIPNRFHRIEEGKLGMYFFQLKPDGTLTVVNPYYV
                     FIPALPLLPEEIEQKLSQAQHPFEKVQIELLALLVHSTQTEPRKDALDLLQQVENPEI
                     NATCHSLLKKAEGPLYQWLLHTLIWREDLSVLEAAVASVLRPISNEEHGYVTGRLCGA
                     IRSKVNDINLLPIILPLLESPDVTPREAGYFAIREMQNKRCIPYLLQAVRDPDKQIRF
                     HGALGLSVMTEQYKWYPGGDDWVNNDRKITDYWIAWGEKYLRRTKKEPGHSPSLDSF"
BASE COUNT         1402 a         1401 c         1495 g         1565 t
ORIGIN      
        1 tccgaaaaat ctttcattcc agcccggtgg caattgcgct gctctcactg acggattcca
       61 aagtcctcga tgtcaatccg gcctttttgc agatgtgtgg gtacacgcgc ggggaagtgg
      121 ttggccgtcc gccgctggaa ctcaatctgt ggatcaatct gtcagaccgc gagcgcctgt
      181 tgcaggcaat cagctcgggc aaaaccccgt ttttgatcga aacgccgttt cggtcaaagt
      241 ctggtgaatt ccgccaggcg tcaggcgcgg ttgagttaat ccatctcgat gaagggccct
      301 gcattctggc catgttttat gacgtcaccg agcggcaatt gcgtgaaggc cagatgcaat
      361 tgcttgaatc ggcggtgttg aactcgctgg acgccatttt gattctggag ccgcaccctg
      421 acaacccgcg tgatattcgg attgtctttg tcaacgaagc ctttacccgg ctgaccggct
      481 atagccgcga agaagccctg ggtcagagcc tggccataat gcacggcccc aaaaccgatc
      541 tgaacgaagt agctcggatc cgggccatcc gcctcgctgg aaaaccgttt tcctccgagt
      601 tgatccacta ccgcaaagat ggcacgccat tctggtgcga agccagttgc atcccagttt
      661 ttgagccgga gcaaaacctg cgccggtggg ttttattcca gcgtgaaatc acggaacgaa
      721 aagaatacga agccaaattg caggaactga accagcattt gattgatacc ctggagcgga
      781 tttctgatgg ctttgttgcc tttaacagcg agatggaaat cacttatgcc aataaagtgg
      841 cagaaaatct gacgggcgtt ccgcttgaga agttactggg gcgaaacctg ctgatttatt
      901 ttccgcaagc gaccaataac tttttttaca ccaattattt gaccgcactg cgcgaacagc
      961 gtcccgttca tttcgaagcc ttttccgaag tgttccgagg ctggtacgaa gtccatgcgt
     1021 acccatcagc caacgggctc tcagtgtttt tccgtgacgt gagcgaacgt tatcgcctcg
     1081 aagccgaata ccgcaaaacc gcgcatcttg aatccctggc cgatcaggtc ggggaagtcg
     1141 cccaggagtt tgaacaggtg ctcacccgaa tgcaggccag cgtccggcat gtccaggagc
     1201 tgacggaagg tctcccgaaa gtgaccaaac cgatggaact cctggttgag tcaagccacc
     1261 agtcaagcca cctggccggg cggctgctgg tcttgtctca agagcgaacc ctgcctgaag
     1321 cgaaagaaga gtcctgagcc tggttgcctc tgaattgcat tgatgagcca aaggttgatg
     1381 acctttggct tttggcgttg gatttgctcg gttttggaag agcgcttcat ttctgtcagg
     1441 ttcattccgc agtgcgtctt gcgcattgtg ttccagcaaa aaatcaagtg ttgatttgaa
     1501 acgacttatt caggttggtt ggctagagtg taagccctgc ctgcaaggag gagacccatg
     1561 acgatgacat ttttagtcaa tcaccggttt gaccgggtga caaaaaaagg gctggttttt
     1621 gctgtcgtgg tgctctgtct tctgatttgc cacagcgcca cacatcggtt ttcattggaa
     1681 gatacatatt ttccggctca gagcaaagca tatgacgggc catgctccat tgacatcaca
     1741 ggtgctggac aagtgaaaaa agctgccgcc ccaaaaacgc atccggattc aggaattgaa
     1801 ctggtgttga ggcaggataa agcctgtttt tatcaggggg gtaacgtgtg gctggaaatg
     1861 aaaatgacca acacctcaaa acagagtgtc gccatcgaac cctgtctcaa tcggtttttc
     1921 acccttccaa ctcaggaaat gcactttgat gtcagaaacg agcgtcacga gcccgttcca
     1981 caattgctga ccgccttccc atccaccatc aggtggtcaa agcaccgaag tactccaaaa
     2041 aaagcaatca aaccgtcaga gaattacagt caatcctttt gcctgaatgt tttatttgat
     2101 ctcactcaac caggtctcta ttcggttgaa cttgagcaac ttgtgcattg tgaagacgga
     2161 acgcccgttc acctgacatc aaaccaggtc tggtttcaga tttttgagga tcagaccata
     2221 tctgagtttg gcgaaccggt ctcagccatt gggttggtta cttctcaagg catgacgtct
     2281 aggggagaaa tcacatgttt gctggcgtac cggttacatc ttgggctcca gaaacaagta
     2341 tttgaaattt gggatccaat caatgtggat ctggcttttt tgaacccaac caaaaaagtt
     2401 atagtcctgg aagactttca catcctgttt atgtgtcttg agtttgaaat tctccgggaa
     2461 aatgggcaac cggtatcaaa aactcaactt tgtcgctatt tggagaaagg tccggctttc
     2521 tttcatgatg aatccaaccg gcttccaccg ctggcgtcag ttttctacag gcttccgctt
     2581 aagaaattgt ttgagatcaa cgagcgtgga acctatgtca tgacggtccg catgaaatta
     2641 cccaacaacc gtgtggcgac ttcaaatcaa atccgtttta ccgtccaggg gagctggctc
     2701 ccaaagtggt tctgaggtga cgtttatgcc caatcacact tcaactcgaa ctttcaaaat
     2761 tgtgcaggaa cacagcccca gacggtgtga ggtatgccat caggctgatc aatttgaccc
     2821 tgagaccagc cactgtttgc gatgtcagac agtgttcaaa ccggttttaa ccgcgaccca
     2881 gagacagtct tcaggtttac tcagattaca aaaccggccc cagcaactga cattttccag
     2941 tgttactttc attgtcaaat cagcactcag tcgcggctca atccggtcgt ttgcgcaagt
     3001 atttggtgtg gtggttgggg ttgaacttcc tctggcagga ctggcctgga tgctcgggcg
     3061 atggggaagc ccatggttgt ggcccctgta tttgattgcg gctccagttt caatgtgggg
     3121 tgccgaatgt gtgatttcca ggctggtcgc cgattcaatt ctgggaaatc agagttccat
     3181 ttttgaagca gttcgcacga tctggaatcg ccggattgca ttcctgagtg acgtgctcga
     3241 cgatgctttc gacggcccct tctatcggtg gtcaatggtt gtggtcggtg tcaccattgg
     3301 ggcattggtt ggtgaggtgt tccgccgtga aatggcattt ggcgtcatgt ttggtttgta
     3361 tctttggatt ggggtgagtg tatgtaccca aagagccttt tcccggagct ttttggtcaa
     3421 tcaggtatca attctcgacc aggaagttga gtctgacgtc tggcacaaaa gctctcagct
     3481 tggcgagcaa cgaatgttca aactgtggaa tgcggccctg tggtttgttc ggattggact
     3541 ggcatcactg gtggtagctc ccagcctgag tcaggttctc agtttgtcca gtccgctgat
     3601 ctggtatccg atgctgccgg tctacctggc tgaacagcaa gtcgtctgtt ttctgattct
     3661 ggtttttctc aaacttctta tcttgccaac atggctggct ttgaaggttc atctctactt
     3721 tgtgcttcgt gagcaatcac ctgtcgcttc gaaataacgt cttcaaaaag gagaaatcca
     3781 aaatgctgcg cctttgcaag tttcgacaat ttgtgtctct ggtcgttgta attttgatgc
     3841 tgtgctttgg tctggaaccc ggtattcagg ctgccgacga gcctccgtct caacgtcagt
     3901 ccaggtatat ggatgaaggc gaacggcttt accgggcgtt taccgacggt tgtcatgttg
     3961 ctctgggtgt cctgaccgaa gttggaccac ggtgtgaaga ttgccctgaa agttcagata
     4021 aggaagtgct gaaccgccgg gtaaagattt atttgactga atggatttgg gggaaacccg
     4081 cagaaacgat gaccgaactc cacaccactc aggtcacaga aattggtgga cacaagtcgt
     4141 catacggggc ctggagtgcc tgggagggtg tgacggttga ggaaggccac gaacttttga
     4201 ttgtctggtg ggagaaaaaa gctctggagt cacagttgtt atggaaacca ggaccagaag
     4261 ttctggtgac tgacaatcag tctaaaatca atcagatacg aaaaatcgcc gatctttttg
     4321 ccaaatacaa agccaaaccc ggcgaatttg cctccctgtt tcaacaggcg ctggatgtga
     4381 attcatctcc acttgacgcc atgacccttg gatttgcttt tgcgttgatt caacacaccg
     4441 gctggaaaca aggcgatgaa ggggcacggt ggctggtctg gttgctgaat atgaatttta
     4501 aacaactgga cccacaggcc tatctgtggg gtctgtttta ccagctaact cagattttcg
     4561 gaatgctgtc cacgacttca cctcaaatgc gcgaagtggt gctccagcaa ataattcaaa
     4621 ctgcctgttc aggtgatctc gtcaaagccg gaagagccat tgcttgcctg cggaagctat
     4681 gtgaatttaa ccttctgaag gaaactcccg gactttcaca aagattgagt tcagaccaaa
     4741 agcgtctttt gtctgagcga taccagtcat tggtttcaca gaagaaattt ccccggagtt
     4801 tgtcatttga aaaccagtgt ggattgattt ccttcgtgcc atttgacagc gaaagttcaa
     4861 gtcaaaaaga atccaacgac ggtgagtgac cgggctcttt tttcgttcgc cgaagatatt
     4921 tctcgcccca ggcaatccag taatcagtga tttttcgatc attgttaacc caatcatcgc
     4981 cgcctggata ccacttatat tgttcggtca tgactgataa tccaagtgcc ccgtgaaatc
     5041 tgatttgttt atccggatca cgaactgcct gcaaaagata cggaatacat cgcttgtttt
     5101 gcatttcgcg gatggcaaaa tacccggcct ctcgtggtgt gacatctggt gactccagaa
     5161 gtgggagtat aatcggtaac aggttgatat cgttcacttt ggaacggatc gcaccacata
     5221 atctaccagt cacatatccg tgctcttcat tgctgatcgg acgcagaaca gaggccacgg
     5281 ctgcttcaag aactgaaaga tcttcgcgcc atatcaaagt atgcagaagc cactggtaca
     5341 atggaccctc tgcctttttc aacaggctat ggcaggtggc attgatctct ggattctcga
     5401 cctgttgaag taaatcaaga gcatctttgc ggggttcggt ttgagtggag tgcactaaca
     5461 gggcgagaag ctcaatttgt actttctcaa atgggtgttg agcctgagag agtttttgtt
     5521 cgatttcttc aggaaggagt ggaagtgctg gaatgaacac ataatagggg ttcaccaccg
     5581 tcaatgtccc atctggtttg agttgaaaga agtacattcc cagttttcct tcttcaattc
     5641 gatgaaatcg gttggggatg ctggagcgaa agtagtcaaa ttccagtcgg tctttcatct
     5701 gtaattcaga cggtgtgtgg atcacccggt caatgtgaaa cctcgcgtgg tgccgatagc
     5761 cttcgatgtt gaatccattt tcaaggtacg tcgtctcttc cagttgatca attgtgagaa
     5821 cctgaccgac gacgattaca tccgcctcct gaacgagttt ctc
//
LOCUS       DAOQDO010000062         5861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8345_length_5861_cov_3.565966, whole genome shotgun sequence.
ACCESSION   DAOQDO010000062 DAOQDO010000000
VERSION     DAOQDO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8345_length_5861_cov_3.565966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1446
                     /locus_tag="PKZ53_01795"
     CDS             154..1446
                     /locus_tag="PKZ53_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNJ39193.1"
                     /translation="MLTLLAVFIIGLFSGWLLDSAGDVSQKVSNQVNSTTTTVETLPS
                     PAPVWAFFPFGVGRLLAYFVLGGGAGLLGTLAFEQAVLNGKIRYLFVIAGLGLLALFF
                     VLHVLVPSPEIALKKFGQTRFGQASLRLVNRWPGPVRYLALGLIDALLPNHITGALIL
                     LAFTSWDSLRSTMLMLSFGFGIFPLSALKVLLPELTAQIVKEVSSALVTVMVCLFAGV
                     FFLRGAGYAFVNGQIVRDDLLHEGHEVSADDSIVRINVPFPQIELTDLEGKKHRLQEY
                     RGKTVVLVTVGTHCPCVEAYRERLNTLAKRYEPAGVIFLGMNPNGNEPEAEIRDHQRK
                     KPFVFPVTIDADQHVADVIKAKCMTETYLIDTNGLLRYHGRIDDNIYYPQDVTDHTLE
                     NALNVVLGGKPQLLTSQVAMGCAIVRKKLPVVGLKKEG"
     gene            complement(1554..2939)
                     /locus_tag="PKZ53_01800"
     CDS             complement(1554..2939)
                     /locus_tag="PKZ53_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ39194.1"
                     /translation="MARIKWVFAVFLCVCVLPVTTLARQSNPEKKLYAFVNVNVIPMD
                     REQVLENQVVLVKNGKISQIGPANHVKIPKKAERIEAGGKFLLPGLIDAHAHLASDTN
                     LPNDLARQWLKLNLANGITTIRNMVGNPSHLVLRDRIARGEALGPTLITSGPPILGFM
                     APNPAEGEQLVTEYKNAGFDLIKVHENLSPQTYDAIVATARKLGIPVAGHVTQTVGLE
                     RALNARQETIEHLDGYIRALLPATVPVEVPPGQIILGPVLQYVETARIPALAQTTKTA
                     GVYNTPTLALFKVIVSDEKPEDLLKWPELRYATPKMRSDFTKQKQSFLNNAIPLEDRL
                     PFTQIRNQLVVGLSQNGAKLLAGSDSPQFFLVPGFALHRELQALVDAGLTPFQALEAA
                     TRTPAEYLKLNKQIGTVEVGKRADLVLVEANPLTSIANASKTAGVMLKGQWISGAELQ
                     RMLAEIAELNK"
     gene            complement(3367..4134)
                     /locus_tag="PKZ53_01805"
     CDS             complement(3367..4134)
                     /locus_tag="PKZ53_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ39195.1"
                     /translation="MKKIQTVIVDDMPLARQKLVRFLNDDPEIEIVAECGNGLEAIDA
                     IISLKPELVFLDIQMPEVDGFEVLNHIGLDHMPAFIFVTAFDHFALKAFEVNALDYLL
                     KPYDRERFVQALNRAKKQLQRTEPEPQDERLLALLKTLNPEQKFLKRLAVKTSGRTIF
                     LMVDEIDWIETAGNYLELHVGKEVHLIRERMNQIESRLDPTRFVRIHRTTIVNLDRIK
                     ELQPLFNGDQTVILRNGNQLTLSRTYRDRLLALMEEG"
     gene            complement(4106..5236)
                     /locus_tag="PKZ53_01810"
     CDS             complement(4106..5236)
                     /locus_tag="PKZ53_01810"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ39196.1"
                     /translation="MPNFQPFFSSGIKAHLVRATGILVCWTFLALIFTPQTYVLHSQS
                     ALPLTWIEALSANLVLFYGWAILTPFVFWFGEQYPIEAPHQFRNTLVLLLLAIPFAMV
                     HMAMLRAANTFVLNIARAAHNPIPLKAVLVGVGATDILVYLGIIGVSQAITYFQKYKD
                     REYRFAQAQLQALKMQIHPHFLFNTLNAISELIYDHPELADRTITQLSDLLRTTLQSS
                     QNQEITLREELEFLKKYLEIQKTLLQDRLTIEFEIDRDVLPARVPNLVLQPLVENAIR
                     HGIAPRISGGAINIRAFRQNGSLRLQVSDDGIGIKGTPEQALGLGIGLPNTRERLKRL
                     YGENQQFEFRTLPDGGMSVHVTIPFHTDFAEVFYEEDSDSDR"
BASE COUNT         1415 a         1415 c         1549 g         1482 t
ORIGIN      
        1 atcgcaaagc ggctgaacag gcggcgaaaa agaatggtgg caaagtgctt gattggaaag
       61 gggttctggc tgaactccgc aagtcgcccg aatttcagtc ccactgatct tcattttgaa
      121 aagcggcgtc aagccgccgt actcacagga tatatgctga cactgcttgc tgtttttatc
      181 attggattgt ttagtggctg gttgcttgat tccgcgggtg atgtctcgca gaaagtttca
      241 aatcaagtca attccacgac caccacggtt gaaactctgc cgtcaccggc gccggtttgg
      301 gctttttttc cgtttggggt cggaagactg ctggcttatt ttgtgctcgg cggcggggct
      361 ggacttctag ggacactggc tttcgagcag gctgtattaa acgggaaaat ccgctatcta
      421 tttgtcattg caggacttgg gttgctggcc ttgttttttg tcttgcacgt gctggtgccg
      481 tcaccagaaa tcgcccttaa aaaatttggg cagacccgat ttgggcaggc aagtctgcgg
      541 cttgtgaacc gatggccggg cccggtgcgc tatctggcgc tcggactcat tgacgctttg
      601 ctgcccaatc acattaccgg tgccttgata ttactggcat ttacgtcgtg ggacagtctg
      661 cgtagcacca tgttgatgct tagttttggg tttgggattt ttcccctgtc tgcattgaaa
      721 gtgctgcttc cagaactcac cgcccagatt gtaaaagaag tttcttcggc cctcgtaacg
      781 gtgatggtgt gcctgtttgc cggggtattt ttcttacgtg gagcgggcta tgcctttgtc
      841 aacggccaga ttgtccgcga tgacctgttg cacgaaggcc acgaggtttc ggctgatgat
      901 tcaattgtgc gaatcaatgt tccgtttcca cagattgaat tgactgatct ggaaggcaaa
      961 aaacatcgcc tgcaggaata tcgcgggaaa accgtggttc tggtgacggt tggcacccat
     1021 tgtccctgtg tcgaagccta ccgcgagcga ctcaacaccc tggccaaacg ctatgaaccg
     1081 gctggcgtta tctttttggg gatgaacccg aacggtaacg aaccagaagc tgaaatccgc
     1141 gaccatcagc ggaaaaaacc atttgtcttt ccagtcacga ttgatgccga ccagcatgtc
     1201 gccgatgtga tcaaagccaa atgcatgacc gaaacttatt taattgatac aaacgggttg
     1261 cttcggtatc acggacggat tgacgacaac atttactatc cgcaagacgt gaccgatcac
     1321 acgctggaaa atgccctcaa tgttgtgctt ggtggcaaac cgcagcttct gaccagtcaa
     1381 gttgccatgg gatgtgccat tgtgcggaag aaactgccgg tggtcgggct gaagaaggag
     1441 ggctgaagac gtggggctca gggcttgggg ctgaagaaga tgggctgaag aattggcttt
     1501 ctttcatccc tcagccctgg ttttcccagc cctcagccct ggttttctca gccctactta
     1561 ttcagttcag cgatttcggc cagcattctc tggagttcgg cacctgaaat ccactgtcct
     1621 ttgagcatca cgccagccgt ttttgaggca ttggcgatgc tggtgagcgg gttggcctca
     1681 accagcacca gatcggcacg ttttccgact tcaactgtgc caatttgttt attgagtttg
     1741 aggtattcgg ctggtgttcg ggtggcagct tccagagcct gaaacggagt gagtccggca
     1801 tcaaccagcg cctggagttc gcgatgcagg gcaaaccctg gtaccagaaa gaattgcggc
     1861 gaatcagacc cggccagcag tttggcccca ttttgcgaga gtcccaccac cagttggttc
     1921 cgaatctgcg taaacggaag ccgatcctca agcggaatgg cattgttgag aaagctctgt
     1981 ttttgtttgg taaagtcaga gcgcattttc ggtgtggcat agcgcagttc gggccacttg
     2041 agcagatctt ctggtttttc gtctgacacg atgactttaa agagagccag cgtcggggtg
     2101 ttataaaccc cagccgtttt cgtggtttgg gccagcgccg gaatccgcgc tgtttccaca
     2161 tactggagaa ccggccctaa aataatctga cccggcggca cctcaactgg gaccgtggct
     2221 ggcagcaacg cccggatgta cccatccaga tgttcaatcg tttcctgcct ggcattgagc
     2281 gcgcgttcga gtccaaccgt ttgagtgacg tgacccgcaa ccgggatccc caattttctg
     2341 gccgtggcca caatggcgtc ataagtttga gggctcaggt tttcgtggac tttaatcaga
     2401 tcaaatccgg catttttgta ttcggtgacc agttgttcac cttcagccgg attgggtgcc
     2461 ataaatccaa ggattggcgg cccggaagta atcaaggtcg ggcccaatgc ttcgccgcgg
     2521 gcaatccggt cgcgaagcac cagatgactc gggttgccaa ccatattgcg aatggtggta
     2581 atgccattgg caagattgag ttttaaccac tgccgggcca gatcgttggg caaattggta
     2641 tcgctggcaa gatgagcatg ggcatcaatc aacccgggca gcaaaaactt gccgccagcc
     2701 tcaatgcgtt cggctttttt ggggattttg acatgattgg ccggtccaat ctggctgatt
     2761 ttgccatttt tgaccagcac cacctgattt tccaggacct gttcccggtc cattggaatg
     2821 acgttaacat tgacaaaagc atagagcttt ttttctggat tggattgacg ggccagcgtg
     2881 gtgaccggga ggacacacac gcaaaggaag actgcaaaaa cccacttgat gcgagccatg
     2941 ccaggaaccc tccgaattta gaactgagtg ggtagggatt gagaaggtca ggaaagaagg
     3001 aaatgaaagc ggtttatcgc ggtacaggtt cttatggtct gaattgttga taccaatttg
     3061 caatgaaatt gttgtaataa aaaacagtca agtgactcag ttaaataagc ttagcgaact
     3121 actgaccact ggccactgac gactgactac caaaaaataa tagccagcac tacgaaaagc
     3181 aaaaaagttg ataaccgtcg cactttgctt cgcttttagt agtgctggct taggccaaac
     3241 atttaccctg catatcaacc gatatgcttg atgtgcagag tactcgcatt cctggaaata
     3301 ggaatttgaa tgtgatcaag cgtgcctggc atgacacgaa ccgtgaaaag aagtacccga
     3361 accgtgtcag ccttcctcca tcaaagccag taatcggtcg cgataggtcc ggctgagcgt
     3421 aagctggttg ccattgcgca agatcacggt ttgatctccg ttaaagagcg gttgaagttc
     3481 tttgatccga tcaagattga caatggtggt gcggtggatg cgcacaaacc gggttggatc
     3541 aagccggctt tcaatctggt tcattcgctc gcgaattaaa tggacctctt tgccaacgtg
     3601 gagttccaga tagtttccag ccgtttcgat ccagtcaatt tcatcaacca tgagaaaaat
     3661 ggttcgccct gaggttttaa ccgccagtcg tttgagaaat ttctgctctg ggttgagcgt
     3721 tttcaaaagt gccagcagcc gttcatcctg tggttctggc tcggtgcgct gaagctgttt
     3781 tttggcacgg ttgagcgcct ggacaaatcg ttcccggtca tagggtttga gcagatagtc
     3841 caatgcgttg acttcaaacg ctttcagcgc aaagtgatca aaagcggtga caaaaatgaa
     3901 ggccggcatg tgatccaggc cgatatgatt caaaacttca aacccatcaa cttcgggcat
     3961 ttgaatatcg agaaacacca gctcgggttt gagcgagata atggcgtcaa tggcttccag
     4021 tccatttccg cattcggcca caatttcaat ttcggggtcg tcattgagaa accggaccag
     4081 tttctgacgt gccagcggca tatcgtcaac gatcactgtc tgaatcttct tcataaaaaa
     4141 cttcagcaaa atcagtatgg aaaggaatgg tgacatgaac cgacatgccg ccatctggca
     4201 gggttcgaaa ttcaaactgc tggttttcgc catacaggcg cttgagtcgt tcgcgggtat
     4261 tgggcaaccc aatacccagt cccagagcct gctcaggcgt ccccttgatt ccaataccat
     4321 catctgaaac ctggagtctg agcgagccat tctggcgaaa agcccggatg ttgatcgcac
     4381 caccgctgat gcgtggggca atgccgtgcc ggatggcatt ttcaaccagc ggttggagca
     4441 ccaggttggg cacccgggct ggcagtacat cgcggtcaat ctcaaattca atcgtgagtc
     4501 ggtcctggag cagcgttttc tgaatctcca ggtacttttt gaggaattca agctcttcgc
     4561 gcaacgtgat ttcctggttc tggctgcttt ggagcgtggt tcgaagcaag tcactcaact
     4621 gggtaatggt tcgatcagcc agttcaggat ggtcatagat cagttcagag atggcattca
     4681 gagtattgaa cagaaaatgt gggtgaatct gcattttcag cgcctgaagc tgagcctggg
     4741 caaagcgata ttcacgatct ttatattttt ggaaatatgt tatcgcctga ctgaccccga
     4801 tgatacccag atacaccaga atatcagtgg cgccaacccc caccaggacc gccttgagcg
     4861 gaatcggatt atgcgccgcc cgtgcgatat tgagcacaaa cgtgttggcg gctcgcagca
     4921 tggccatatg caccatggca aaaggaatgg ccagcagtaa taacacgaga gtgtttcgaa
     4981 actgatgtgg ggcttcaatt gggtattgtt cgccaaacca gaacacaaac ggggtcagga
     5041 ttgcccagcc gtaaaacaac accagattgg cactgagtgc ttcgatccag gtgagtggca
     5101 gggccgactg gctatggagc acataggttt gcggggtgaa aatgagcgcc agaaacgtcc
     5161 agcacaccaa aataccagtg gcgcgaacca gatgggcctt gatccccgat gaaaaaaatg
     5221 gctgaaagtt tggcatcaca gagaagaaag aattgagagc gaaggcggca gaatacagaa
     5281 aagataaagt ggatccctca ttctgtcttc ctccgttcac gactcatttt gtttctgaaa
     5341 atgttcaatg gcgttgattt cgtttaggga cagtcgaaat ttactgacgc cattgatacc
     5401 ggctccctgg tgtggatttc agccccccac cgtagtgcca gtaacggctg gttgccgtaa
     5461 ggtcgaggcg agtgcgactt cgcctggtgt ttgaccctgc ttgtgaccaa tttcaccaag
     5521 aaaatcagcc atttgcaagt aaaagtccaa agttctggcg ttcgcctgca aaaacatggg
     5581 ctgagatcat tttctggaaa gttgaccatc ggcgtagttt ggcgaaaaat agagctgtcg
     5641 gttgctcaca ttcaccgtca tcgaatcaga gtcaactcaa tcgaaacaac cgttcttcac
     5701 ttcttttgcc aggacaccaa tggatacaat tgtttacctt gatcgcgaag ccattcgcgc
     5761 accattgcgc aggccagatt ttccacatca ctggattgaa tatcccaata gttccgaatt
     5821 ttattataat ttataaaatc ggtttcacct ggcggagcgg t
//
LOCUS       DAOQDO010000063         5837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8405_length_5837_cov_3.331546, whole genome shotgun sequence.
ACCESSION   DAOQDO010000063 DAOQDO010000000
VERSION     DAOQDO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8405_length_5837_cov_3.331546"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PKZ53_01815"
     CDS             complement(<1..1134)
                     /locus_tag="PKZ53_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39197.1"
                     /translation="MRLRLICPRLVKMYLGLLVVLLTPPTILGSTAHFLTSTCQVPTA
                     SISSPTQSDQPSSEFSQDLSKPQVRDLKGGEVHTYQVVLEAGQYWQAVVEQQGIDVLV
                     TVFDPASKQVYFLDSPDGRQGPERIFLVAATSGLHRLEVRPADPAAEPGQYTIHAEPV
                     RKATSLDQARMDGELAFGQGMQLYQQSTKESFEQALVQYDLAQRAFQTSGDKSLEAQT
                     LMFKVLATDTLGNKEQGLKLAYELAALWKALDNRIEEARTLNYIGLTHNTQGNHLKAL
                     EILNQAADRLKGIQNPSVEALTKTNLGVVYGDLGETRKALEAFTTAQPVWRELHDKFN
                     ENTGLLNIGQSLYQLGETPKAIESIEAALRLARDAGEGHAQVIH"
     gene            complement(1153..2310)
                     /locus_tag="PKZ53_01820"
     CDS             complement(1153..2310)
                     /locus_tag="PKZ53_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNJ39198.1"
                     /translation="MSASKLNNPELVELIQDTAEYIRYLKTLGVSHLGGAADIPVDIP
                     KPAPRTSLVSSQPALLPAEHAAELPPVLPVEAPQSEPVSVETESLPVDEPPPVTRRSL
                     SVSEPVEAASPLLPVESEPMPQPAAEITPAPPEPSESVSPELAPEIPTMRLIKVTPTA
                     PAKTTAKSKPQASLFDMFGAEPEPPAPEPEPEPAAQDTCLQDIRDEIGDCQRCKLATG
                     RHHIVFGDGNPNARLVFVGEAPGADEDASGVPFVGRAGQLLNKIIEAIGMKREDVYIC
                     NVVKCRPPENRTPERDEIDACQGFLHRQLAIIKPKIIVSLGAPAAFTLLGDSRIGRIS
                     EIRGNFYDYRGTPLMITFHPAYLLRTPEKKREVWEDMKKVRAFLRGELKLE"
     gene            complement(2518..3570)
                     /locus_tag="PKZ53_01825"
     CDS             complement(2518..3570)
                     /locus_tag="PKZ53_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39199.1"
                     /translation="MEETISRDDYDSPWKEALDEYFKDFLELLFPMVHDGIDWSVPPQ
                     SRDKEFQKVTATAKQGRQVADKLKEVQTLDGKTRWVLVHIEIQSQWEADFAERIFRFD
                     ARIYDEFDKQVVSLVVLGDGLPDWKPNRFGWSFWGYEMAVRFPTVKLINLHVEWLEQS
                     QNRFAPIVLAHLKTMETKNEDQTRREWKFRIVRSLYERGYSAEDVRKLFRIVDWFMQL
                     PEELEHKFQTDLEEYEEQHHMPYITSIERMAEARGEARGEARGEAQGRIQGEAQGRIQ
                     GEAQGLVKGRIQSKIESLLILLTTRFGVISAATTQIIQSIDDEARLTDLLRTVVMVSS
                     LDEFLVELGKNEPNGA"
     gene            3787..>5837
                     /locus_tag="PKZ53_01830"
     CDS             3787..>5837
                     /locus_tag="PKZ53_01830"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39200.1"
                     /translation="MRKSNILKVFSTCCIQVRWLIPVVGLISGWFCYLHPVQTIQAQQ
                     GPKPFQSEQTVFEDDPEARADWFWQERLYPTNSIPTGARVKAIRERQRVEASLRESRT
                     IAGNEWRSIGPAPAKSLPPEWGNVSGRISAIAVHPTNRQVIYIASATGGVWKTENGGA
                     SWTPLTDFEPSLTAGAIAIAKADPNVIYVGTGDVNHVNVLNQPRYFGAGVLKSTDAGA
                     TWEHLAFAELEYAAISKVIIHPKNPDIVWIGVASSRDSETGDVQPTGVWLTTNGGKTW
                     TRTLQLTEVSGIHDLAINPLDGNVVYAAASYPGSSDNGVYKSTDGGKTWQLKSTGLPP
                     LPLIRRMRLALAPSSPNILYASILGTAQYFGVYKSTDSGETWELLPNSSIFCGLQCWY
                     DHYIAVAPNDPMVIYLGEIDVIKSIDGGRSWSNLVVGVGNIHPDQHAIAFDPVDPNIV
                     YFGCDGGMYQSRDAGLSFADINGNLALTQHVGLALHPTDPNIMFGGTQDNGTLKNTGS
                     LVWQQVSYGDGGYTVVDYENPQTVYTTYIYTSILRSLNGGNSFVDLTIPETFSRERAS
                     FYAPIVIDPINPKALYFGTYRLWRTEDMGRHWARTSEQDLTSTPLGTISAIAVSKLNP
                     KLVYVGSSDGVLSRSTDGGATFNRVAGIPDRVIRRVTIDPADPRVAFVAVSGFGTGHV
                     F"
BASE COUNT         1337 a         1416 c         1554 g         1530 t
ORIGIN      
        1 atggatcacc tgcgcgtggc cctcgccagc gtcgcgagcc agtcggagcg ccgcttcaat
       61 tgattcaatg gcttttggtg tttcaccaag ctggtacaga gactggccaa tattgagcaa
      121 tccggtattt tcgttgaact tatcgtgaag ctcgcgccag acgggttggg ccgtggtaaa
      181 ggcttcgagc gctttgcggg tttcgcctaa atcgccatac accacgccga gattggtttt
      241 ggtcaaggct tcaaccgatg gattctgaat tcctttcaaa cggtcagcag cttgattaag
      301 gatttccagg gctttgaggt gattgccctg agtgttgtgg gtcaacccga tatagttgag
      361 tgttcgggct tcttcaatcc ggttatccag agccttccaa agagcggcca gttcataagc
      421 aagctttagc ccctgttctt tattgcccag ggtatcggtt gcaagaactt tgaacatcaa
      481 cgtttgggct tcgagagatt tgtcgccact ggtctgaaac gcccgttgtg ccagatcata
      541 ttgaaccagt gcctgttcaa atgattcttt ggtgctttgt tgatacaact gcatgccctg
      601 accaaacgcc agctcaccat ccatccgtgc ctgatccaga ctggtggctt tgcgcactgg
      661 ttcagcgtgg atggtgtatt gtccaggttc cgcggctggg tcagcgggcc ggacttcaag
      721 tcggtggaga ccgctggttg ccgcaaccag aaaaattcgt tctggtccct gccttccgtc
      781 tggactatca agaaaataga cctgtttgct ggcaggatca aaaaccgtga ccagaacgtc
      841 aattccttgt tgctcgacca ctgcctgcca gtattgcccg gcttcgagaa ccacctgata
      901 ggtgtggact tcgccgcctt taagatcacg aacctgtggt ttggataaat cctgggagaa
      961 ttcagatgat ggttgatcgg attgagtcgg tgaactgatt gaagcggttg gtacctgaca
     1021 ggttgaggtg aggaagtgag cggtgcttcc caggatggtt ggtggggtga gaagcaccac
     1081 aaggagtcca aggtacattt tgaccaggcg ggggcatatc aggcgaagtc gcatacagaa
     1141 ctctccggaa aatcattcaa gtttgagttc gccacgcaag aaggcgcgaa cttttttcat
     1201 gtcttcccat acttcgcgtt ttttttcagg agttcggagc aaataagccg gatgaaatgt
     1261 gatcatcagt ggcgttcccc gataatcata gaaattgccg cgaatttcac tgatgcgtcc
     1321 aatgcgggaa tctccgagca gtgtgaaggc tgccggagca ccgagcgaaa caatgatttt
     1381 tggtttgatg atcgccagtt gacggtgcag aaagccctga caggcatcaa tttcatcacg
     1441 ttccggggtt cggttttcag gtggacggca tttgaccacg ttgcagatat agacatcctc
     1501 tcgtttcatg ccgatggctt cgatgatttt gttaagaagt tgacccgctc gcccgacaaa
     1561 cggcacgccg ctggcatctt catctgcacc gggagcttca cccacaaaca ccagtctggc
     1621 atttggattt ccatcgccga agacaatgtg gtgacggccc gtggccagtt tgcaccgctg
     1681 acaatcgcca atttcatcac gaatgtcctg aaggcacgta tcttgcgctg ctggttcggg
     1741 ttccggttct ggggctggtg gctcgggttc ggctccaaac atatcaaaca acgaagcttg
     1801 cggctttgat tttgccgttg ttttggctgg ggcggttggt gtgactttga tcaggcgcat
     1861 ggtgggaatc tccggagcta gttcaggtga cacggattca gagggttcag gtggcgctgg
     1921 agtgatttca gccgccggtt gtggcattgg ctcagattca accggcaaca ggggtgaggc
     1981 tgcttccact ggttctgaaa ccgataggga acgccttgtc actggcggtg gctcatcaac
     2041 tggcagtgac tccgtttcga cagaaactgg ctcactctgc ggtgcttcaa ctggaagaac
     2101 tggcggtagt tcggcggcgt gttctgcggg caacaaggct ggttgagatg aaacaagaga
     2161 cgttctgggg gctggtttgg gaatatcaac cggaatgtcg gcggcgccgc caaggtgcga
     2221 gaccccgagg gttttgaggt aacggatata ttcagcggta tcctggatta gttcaacaag
     2281 ttcaggattg ttcagctttg aagcagacac ggttgggcat ccttttcggg ttcagggttt
     2341 tcggaaggga tgaaggatga aggatgaggg atgaaagaaa tcatcttgtc accttgtcat
     2401 tcggtcatct ggtcagggtt caaggttcag ggtttataga aagttattta tatcagtgag
     2461 ttagcttgtt gctgaaccga agttctgatt tcaaaatggc atcagtcgtt ttatgcttca
     2521 tgctccattg ggttcgtttt ttccaagctc aaccaaaaat tcatcgagtg aagaaaccat
     2581 caccaccgtt cgcaggagat cagtgagtcg cgcctcatca tctatggatt gaattatttg
     2641 tgttgtcgct gcggaaatga caccaaaccg ggtggtgagg aggatcaaca gcgattcaat
     2701 tttggactga attcggcctt tgaccaaccc ttgggcttca ccctgaattc tgccttgggc
     2761 ttcaccctga attctgcctt gggcttcacc gcgagcttca ccgcgggctt caccgcgagc
     2821 ttcggccatt cgttcaatac tggtaatgta tggcatatga tgttgctcct catattcttc
     2881 caaatctgtc tgaaatttgt gttcaagttc ttcaggcaat tgcataaacc aatccacaat
     2941 gcgaaacaat tttcgcacat cttcagccga atagcctcgc tcatagagtg accgaacaat
     3001 tcggaatttc cattcacgcc gggtttggtc ttcgtttttg gtctccatgg ttttgagatg
     3061 ggccagtaca attggggcaa accgattttg actttgttca agccattcaa catgcaagtt
     3121 gatgagtttc accgttggaa accgaacggc catttcatag ccccagaatg accagccaaa
     3181 ccggtttggt ttccaatctg gcaatccatc ccccaatacc accagactca caacttgttt
     3241 gtcaaattca tcataaatcc gggcgtcaaa tcggaaaatt cgttcggcaa agtctgcttc
     3301 ccactggctc tgaatttcga tgtggaccag cacccatctc gtttttccat cgagggtttg
     3361 cacttctttg agtttatcag ccacttgccg accttgtttg gcggtggcgg ttactttctg
     3421 gaactcttta tcacgagatt ggggaggaac actccagtca attccgtcat ggaccattgg
     3481 aaagagcaac tccagaaaat ccttaaaata ttcatcaagt gcttctttcc aggggctgtc
     3541 atagtcgtca cgagagatgg tttcttccat gttttggttc ctgtctttta aactttcaga
     3601 aagttggtta accccaaaca gaattcaggc aagatcaaag tgattgatgg caaaaccagg
     3661 atggcgaaag agtaccacag agccgcttgc caaagcatcc ttgagtcttt ccacacggat
     3721 cggctatggt tgcaacgcct ttataaaaaa tcccacgcta ttttactctg agaaatatcc
     3781 agttttatgc ggaagtccaa tattttaaaa gtgttttcca cctgttgtat ccaggtcagg
     3841 tggctgattc cagttgtcgg actgatcagt ggatggtttt gttatttaca tccggtgcaa
     3901 accattcagg cacaacaagg gccaaaacca tttcaatcag agcaaactgt ttttgaagat
     3961 gatccggaag ccagagccga ctggttctgg caagagcggc tgtatccgac caattcgatt
     4021 ccaaccggag cgcgagtcaa ggccattcgc gaacgccagc gcgttgaagc cagtctgcgt
     4081 gaatcccgta cgattgctgg caacgaatgg cgctcgattg gaccggcacc agctaaatca
     4141 ctcccgcctg aatggggcaa tgtttcggga cgtatcagcg caattgcggt gcatccgacc
     4201 aaccgacagg taatttatat cgcttcggcg accggaggtg tctggaagac cgaaaatgga
     4261 ggcgcaagct ggacgcccct gactgacttt gagccgtcgt tgactgcagg ggcaattgcg
     4321 attgccaaag ccgatccgaa cgtgatttat gttggaacgg gtgatgtcaa ccacgtcaat
     4381 gtgctcaatc agccgcgcta ctttggcgct ggtgtgttga aatccaccga tgctggtgcg
     4441 acctgggaac atctggcctt tgccgagctg gaatatgccg cgatttccaa agtcattatc
     4501 catcccaaaa acccggacat tgtctggatt ggtgtggctt cctcgcggga tagcgaaacg
     4561 ggtgatgttc aaccgacggg agtctggctg acgaccaatg gtggaaaaac gtggacccgg
     4621 acgctccaac tgaccgaagt cagcggtatc cacgatctgg ccatcaaccc gcttgacggc
     4681 aacgtggttt atgcggcggc ttcgtaccct ggctcatcgg acaatggtgt gtataagtcc
     4741 acagatggtg gaaaaacgtg gcaactcaag tcaactggat tgccaccgtt gccgttgatt
     4801 cgccgaatgc ggctggcact tgcgccttcc tccccaaaca tcctctatgc ctcaatttta
     4861 gggacggccc aatatttcgg tgtctataaa tcaactgaca gcggcgaaac ctgggaattg
     4921 ttacccaaca gcagcatttt ctgtgggttg caatgctggt atgaccacta tattgcggtg
     4981 gcaccaaatg atccgatggt gatttacctg ggagaaattg acgtcatcaa atcaattgat
     5041 ggaggccgtt cctggtcaaa tctggtggtt ggcgtgggaa atatccatcc tgaccagcat
     5101 gccattgcct ttgacccggt tgacccaaac atcgtgtatt ttgggtgtga tggcggcatg
     5161 tatcaatctc gtgatgccgg gctgagtttt gccgatatca atggcaatct ggcactgacc
     5221 cagcacgttg ggttggctct gcatccgacg gacccaaaca tcatgtttgg cggcacccag
     5281 gataacggca cgctgaaaaa cactggaagt ctggtctggc agcaggtgag ttatggtgat
     5341 ggcggctata ccgtcgttga ttatgaaaac ccacaaactg tttacacaac ttatatttac
     5401 accagtatct tgcggtcgct caatggtggc aatagttttg ttgacctgac cattccagag
     5461 acattcagcc gtgaacgggc ctcgttttat gctcccatcg tcattgaccc gatcaatcca
     5521 aaggcgctgt attttggaac atatcggctc tggcgcaccg aggatatggg ccgccactgg
     5581 gcccggacat cagagcagga tttaacctcg acgccactgg gtactatttc agcgattgcc
     5641 gtatcaaaac tcaacccaaa gctggtgtat gtcggctcaa gtgatggtgt gctgagtcgt
     5701 tcaaccgatg gcggggcgac cttcaaccgg gtggctggga tccctgatcg ggtgattcgc
     5761 cgggtaacga ttgatccggc tgatcccagg gtcgcttttg tggcggtctc ggggtttggt
     5821 accggacacg tctttaa
//
LOCUS       DAOQDO010000064         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8485_length_5805_cov_2.624000, whole genome shotgun sequence.
ACCESSION   DAOQDO010000064 DAOQDO010000000
VERSION     DAOQDO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8485_length_5805_cov_2.624000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..759)
                     /locus_tag="PKZ53_01835"
     CDS             complement(127..759)
                     /locus_tag="PKZ53_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNJ39201.1"
                     /translation="MNIAVIGSGNVGGTLGRRWAEAGHQVTFGARNPDNPKLQAVLAQ
                     TNHQARGASIPEAIANAEIVVLTTPYEAAKEVLQPASSLAGKIVLDCTNPIAPGLKGL
                     TVGLNTSGAEELARLIPETPVVKSFNTTGWENMANPHYGDHKATMFLCGDNPEAKQKV
                     IQLIEVLGFEASDTGSLHMARYLEPLAMVWIHLARIQGHGADIALKLLKR"
     gene            1061..1492
                     /locus_tag="PKZ53_01840"
     CDS             1061..1492
                     /locus_tag="PKZ53_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ39202.1"
                     /translation="MPHSSPELPAPQSQLAIYLVANKWRLAVIHALSTGTLRYGEIHR
                     ALGAVSHKMLAQTLRGLERDGLLARHHFSEIPPKVEYSLTPLGESLREPLRQLCYWAQ
                     DHAEEIRLAQERFDQTHIVYRDAPLVELAKTEGAGKEKLTI"
     gene            1689..3134
                     /locus_tag="PKZ53_01845"
     CDS             1689..3134
                     /locus_tag="PKZ53_01845"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNJ39203.1"
                     /translation="MADKPVIPDATESGEPGKPQATPVDSQSAEQTAVAETAQVASGQ
                     DVPTLAESATDSEIQDPVSKTAVDAPEFDISPLESLIFSMRTSSPMHVTMDLPEAKED
                     LIELEHPLGHPEAPIHLSSLGVEEEPFIDPGLPIPEAYDADMISALVQDPFRIYIYWE
                     IRPETLRSINKLFTPAEVKTFQPVLRVTDKTTEEETYYPVKIPSSDWLSVSPGRTYIV
                     EFGVYSPNFGFVPLMAAPEVTTPRGTVAPPSTEPDFQISTARFKKLLRASGFSAHAGA
                     LPHETLADWLPPHVTDAFIQAGAGEPLQDIHLEAIPETVRDILAELVAHGEGEMAANS
                     LLHYLPSVLRDAYVAYLAGRGMPTGIRNVEHLRDLVGPVDLEGEGVAESEDWTEYEYS
                     EWEEVEDEDFFAPGLLRFGIGSSAGSELGSLHGRRRRRLPRRAPRRRPVLKQAERPAP
                     WLPSMDRPASSGGRALLLPMDLDVPPLPDEI"
     gene            3335..4915
                     /locus_tag="PKZ53_01850"
     CDS             3335..4915
                     /locus_tag="PKZ53_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNJ39204.1"
                     /translation="MVTGFLALILHAHLPFVRHPEHPEYLEEDWLYEAISETYVPLLF
                     SFSRLHKAGVKFRVTMTMTPPLCEMLADALLQERYSKHLAKLIELTTKEVERTAGTEF
                     ENAARAHHEHFSGVQDLYENLYQRDLIRAFRELQDAGVLEIITCGATHGFLPLMYRRE
                     ARRAQIQVAVRNYEKHFGRRPLGIWLPECAYQPGIDELLAEAEIKYFLVDSHSIMFGT
                     PRPMRGIFAPVITPSGVAAFARDVDTSEQVWSSVIGYPGHPNYREFYKDLGYEAEEEY
                     IKPYLHLDGVRRNVGIKYYRVTGKVDLHEKQPYEPAWATEVAAEHAGHFLASRQAQMH
                     NLRPLIGRAPLVVSPYDAELYGHWWYEGPQFLDFLIRKIHYDQDEVHLITPADYLEMF
                     PENQPQTPSASSWGAEGYNRVWLNRATQWMYRHQHIAENRMVDLLNRFPEAEGLNLRA
                     LNQAARELLLAQSSDWAFIITNQTMVAYAVKRFRDHIHRFTKLYEFLTTDTIEEEWLA
                     EVESRDNIFPEIDYRVYQ"
     gene            complement(5009..5082)
                     /locus_tag="PKZ53_01855"
     tRNA            complement(5009..5082)
                     /locus_tag="PKZ53_01855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5048..5050),aa:Gln,seq:ctg)
     gene            5328..>5805
                     /locus_tag="PKZ53_01860"
     CDS             5328..>5805
                     /locus_tag="PKZ53_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39205.1"
                     /translation="MPKRRVVFKLLIGVLVAVTVLQCKFETFFNPVAHGATASQPQSP
                     SPSEGQEDGDAPIVVASVVNVTLPVTVLNEDGNFVRGLKQNDFQVFENRQQQRVVSFQ
                     KQDGLTLNLTLAMDISTSVRNRLAFEKEAIKEFLERLLDNKRDQASLVTFNEKVDFR"
BASE COUNT         1381 a         1489 c         1470 g         1465 t
ORIGIN      
        1 aagaattggt tttatttcat ccctcatcct tcatccctca tcccttcgat tgccctgagc
       61 ttgcaagtct tcagccccaa gccctcagcc caccttcttc agcccgatgt cttcagccct
      121 ggtttttcag cgtttcaaaa gcttcaatgc gatgtctgct ccatggccct gaatacgggc
      181 cagatggatc cacaccatgg ccagcggttc cagataccgg gccatatgca ggctgccggt
      241 gtcagaagct tcaaacccaa gtacttcaat gagttgaata actttttgtt tggcttccgg
      301 gttgtcaccg cacaaaaaca tggtcgcttt gtggtctcca tagtgtggat tggccatatt
      361 ttcccagccc gtggtgttaa aactttttac aacaggagtt tctgggatga gcctggccag
      421 ctcttcagcc cccgaagtat tcaaccctac cgttaaccct ttgagcccag gggcaatcgg
      481 gttggtgcag tcgagtacaa ttttgccggc gagtgaactg gcaggctgca atacctcttt
      541 ggctgcttca tacggcgtgg ttaacaccac aatttctgca ttggcaatgg cttcgggaat
      601 gctggctccc cgagcttgat gattggtttg ggcgagcact gcctgaagtt ttgggttgtc
      661 cggattgcgg gcgccaaatg taacctgatg acctgcttcc gcccaacgac gaccaagcgt
      721 accaccgaca tttcctgaac caataactgc aatgttcaca attgagtctc cttaattgaa
      781 gggtattctt gaagggccga aaaatcctgg ggctaagggc tgaagaccca ccagctcggg
      841 gcaattgaag ggatgaggga tgagggatga gggatgaggg atgaaagaaa accaattctt
      901 cagcccaact tcttcagccc caagccctca gccctggttt tctcagcccg agttctttag
      961 cccgaattgt attgaatgtc gccgaatatg tccttaaact ggaaaccaca caagtacgca
     1021 ctttccggtg attaacttac ccaaaggaaa gtactctgac atgccccatt catcacctga
     1081 actgccggca cctcaatcac aactggccat atatctggtt gccaacaaat ggcgactggc
     1141 ggtgattcac gccttatcca ccggcacatt gcgctatggt gaaattcatc gtgcattagg
     1201 ggccgtttcg cataaaatgc tggctcaaac gttgcgtggc ctcgaacgtg acggactgct
     1261 ggcacgtcat cacttttctg aaatcccccc caaagttgaa tactcactca cccctctcgg
     1321 agagtcactc cgtgaaccgc tccgacaact gtgttattgg gctcaggacc acgctgagga
     1381 gatccgtctg gctcaggaac gctttgatca aacccatatt gtttaccggg atgcacccct
     1441 ggtcgagttg gcaaagactg agggtgctgg aaaagaaaaa ctgacaatct gagcccgaac
     1501 gtggtagagt ctcacctttt attttctatc caaccgtttt cactgtcttt gccgttcagg
     1561 tcattcattt gaattcatat cagtttctgc tcagcgaaag acaaagcggt ggagcgtgtt
     1621 ttataccgag cgctgttggt cagactgaca gccaccactg atactttgca attttaagga
     1681 gagctcatat ggctgataag ccagttattc ctgatgcgac tgagtctggt gaacctggga
     1741 aaccgcaggc cacgccagtt gattctcaat ctgcagaaca gacagccgtt gccgaaacgg
     1801 ctcaggttgc gtcaggtcag gacgtcccca cactggccga atccgctacg gattccgaga
     1861 ttcaggaccc ggtttcaaaa acagcagttg acgcccccga gtttgatatc tcgccgctgg
     1921 aatcgttgat cttttccatg cggacgtcct cgccaatgca cgtcacgatg gatcttccag
     1981 aagccaaaga ggatttgatc gaactggaac atccactggg gcacccggaa gccccaattc
     2041 atttaagtag ccttggggtg gaagaagaac cctttattga tccaggattg cccattcctg
     2101 aagcctatga cgccgacatg atttcggccc tggttcagga tccgttccga atttatattt
     2161 actgggaaat ccgcccggaa acccttcgct caatcaataa attattcacg cctgcagagg
     2221 tgaaaacctt ccagccggtg ttgcgtgtga ctgacaagac cactgaagag gaaacctatt
     2281 atccagtcaa aattccaagc agtgactggt tgagcgtttc acctggtcga acctacattg
     2341 tcgagtttgg cgtctattca cccaattttg gttttgtgcc gttgatggct gcccctgaag
     2401 tcaccactcc acgtggaaca gtggcgccac cgtcaacgga acctgatttt caaatcagca
     2461 cggcacgttt caaaaaattg cttcgggctt cagggtttag tgcccatgct ggcgcccttc
     2521 ctcacgaaac gctggctgac tggctcccac cgcatgtgac tgacgccttt atccaggctg
     2581 gtgctggtga accactgcag gacattcacc tggaggccat tccagaaaca gtgcgcgata
     2641 tcctggccga actcgttgcc cacggggaag gtgagatggc agccaattcg ttgctgcatt
     2701 acctgccttc agtcttgcgc gatgcctatg ttgcctatct ggctggccgt ggaatgccaa
     2761 ctgggatccg caacgtcgag cacctccgtg atctggtcgg acccgttgat ctggaaggcg
     2821 aaggggtggc tgagagtgaa gactggaccg aatatgagta cagtgagtgg gaagaagtcg
     2881 aagatgaaga tttctttgcg cctggactct tgcgatttgg cattggcagt tcggctggca
     2941 gcgaactcgg gagcctgcat ggccgccgtc gccgtcgcct tccacgtcgt gccccgcgcc
     3001 gtcgtccagt cctcaaacag gctgagcggc cagcaccctg gctgccaagt atggatcgtc
     3061 cagccagcag tggcggtcga gcccttctgt tgccgatgga tctcgatgtc cccccgttgc
     3121 cagatgagat ctaacgtatt tgggggtggg agtttctgat cactgtttca aatgggttta
     3181 agcgcttgtg ggtggggttt ctttctgatt gaaaaaagtc agactgttac cccgccccgt
     3241 atccattgac cagtggccgt gactttccca cccgacgtct tccgataaca acacacaacc
     3301 ccacagacac gtccttttca accacaggag aactatggtt acgggatttc ttgccctgat
     3361 tttgcacgct cacttgccgt ttgtccgcca tccagaacac ccagaatatt tagaagaaga
     3421 ctggctctat gaagcaatca gtgaaaccta tgtcccactg ttgtttagtt ttagccgatt
     3481 gcacaaagcg ggtgttaaat ttcgtgtgac tatgacgatg acaccgccac tttgtgaaat
     3541 gctggcggat gcgttgctac aggaacgcta ttcaaaacat ctggccaaac tgatcgagtt
     3601 gacaaccaag gaagttgagc gtaccgcagg cactgaattt gagaatgctg cccgggccca
     3661 tcacgaacat ttcagcggtg ttcaggacct gtatgaaaat ctgtatcagc gggatctgat
     3721 tcgtgctttt cgcgagttgc aggatgccgg agtacttgaa atcatcacct gcggcgccac
     3781 gcacgggttt ttaccattga tgtatcgtcg cgaggcccga cgcgcccaaa tccaggtagc
     3841 cgtgcgcaac tatgaaaaac attttggacg ccgcccgctt ggaatctggt tgccagaatg
     3901 tgcctatcag ccagggattg atgaacttct ggccgaagct gagatcaagt actttctggt
     3961 tgactcgcat agcatcatgt ttggaacgcc acgtccaatg cggggcattt ttgcgccagt
     4021 gattacgcct tctggcgtgg ccgcctttgc ccgtgacgta gacaccagcg aacaggtctg
     4081 gagttctgtg attggctatc ccggtcatcc caactatcgt gagttctata aagatctggg
     4141 atatgaagcc gaagaggagt acatcaaacc ctatctgcac ctggatggtg tgcgccgaaa
     4201 cgtcggaatt aaatattatc gggtcaccgg taaggttgat ttacacgaaa agcagccgta
     4261 tgaacccgcc tgggccactg aagttgccgc tgaacacgct ggtcactttc tcgcctcgcg
     4321 tcaggcacag atgcacaacc tgcggcccct gattggccgg gcaccgctgg tcgtctcgcc
     4381 ttatgatgct gaattgtatg ggcactggtg gtatgaaggt cctcaatttc tggacttcct
     4441 gatccgcaaa attcactatg atcaggatga agtgcatttg atcacgccag ccgattattt
     4501 agagatgttt ccagagaatc agcctcagac cccgtcggct tcttcctggg gagctgaagg
     4561 gtataaccgt gtctggctca atcgtgcgac tcagtggatg tatcgccatc agcatattgc
     4621 cgaaaatcga atggttgatt tgctcaatcg gtttccagag gcggaagggc tcaacttgcg
     4681 ggcacttaac caggctgccc gcgaactctt actggcacaa tcaagcgact gggcctttat
     4741 catcaccaat cagacgatgg tggcctatgc tgtaaaacgg ttccgcgatc acattcaccg
     4801 ctttaccaaa ctgtatgaat ttttaacgac cgatacgatt gaggaagaat ggctggctga
     4861 agttgaaagc cgggacaata tttttccaga gattgattat cgggtctacc agtaaatatt
     4921 gaaggggaga ggggataggg ggcagaaaag tttgatggtc tctatgccct gaacctgagg
     4981 ggaaggaatc ggttggtggg caaaaaaatg gctgggaggc agggattcga accccaataa
     5041 tacggttcag agccgtaggt cttaccatta gacgacctcc cagtacagaa ccggcaatat
     5101 agacagtttt tctcagatct gtcaagtacc tctttaatct ggctggccgg gaagtgccac
     5161 tcttctttca gaaaaaccaa aacggcaacg tgagttccag aaagggcatc gcaccagggt
     5221 gtccaggttt ctgatttaaa attccaagga aaatttattt tgatttatgg gaagctatgg
     5281 taaggaaaga acttccctct ttattttatt ggttcaggtt atttcacatg cccaaaagac
     5341 gagttgtatt caagcttctg attggtgtat tggtggccgt caccgttctt cagtgtaaat
     5401 ttgagacttt tttcaacccg gttgcccacg gtgcaaccgc atcacaacct caatcgccat
     5461 caccatctga aggtcaggag gatggcgatg ccccgattgt tgtggcctca gtggtcaacg
     5521 tgacgttgcc cgtgactgta ctgaatgaag acggtaactt tgtgcgtggg ttgaagcaaa
     5581 atgattttca ggtctttgaa aatcgccaac aacagcgggt tgtcagtttc caaaagcaag
     5641 acggattgac tctgaatctt accctggcca tggatatcag taccagtgtg cggaaccgac
     5701 tggcttttga aaaagaagcc attaaagagt tcctggaacg gttgcttgat aacaagcgcg
     5761 accaggcttc gctggttacc tttaatgaaa aagttgattt tcgcc
//
LOCUS       DAOQDO010000065         5803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8495_length_5803_cov_3.656054, whole genome shotgun sequence.
ACCESSION   DAOQDO010000065 DAOQDO010000000
VERSION     DAOQDO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_5803_cov_3.656054"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKZ53_01865"
     CDS             <1..280
                     /locus_tag="PKZ53_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39206.1"
                     /translation="NLGGTDATQAGFLDNTITQSSAGTAKSGYLLTYSSTPAAAGAAP
                     TYTCQNTPITRTGITRTGNDSFFVNETGVITHSGDPSNNATSASGPIE"
     gene            525..1736
                     /locus_tag="PKZ53_01870"
     CDS             525..1736
                     /locus_tag="PKZ53_01870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ39207.1"
                     /translation="MSTSPSKETEGFVRPTSKRLAHRFDEVGFSEIVRIRNRVMELKA
                     TGETIFQFEGGEPYMNTPDFVKEAATQALHENKTRYAPSSGIKPLVDAITEKLRTKNK
                     IPAQPEHVIVGNGGMQGLYGAFNSILDPGDEVLLFSPHWTPTRDLIYMSGGQVVSINT
                     FEARQTGISQTLQAHLSPKSRAILLNTPQNPTGIVFTRAEIEEVAAFAQNHDLVVIAD
                     EAYEDLVYEQEHVSIASLPGMYERTISVYTLSKSYAMTGWRLGYAIAAEPFITGLKKS
                     ILYSSNGVSTPTQWAAVAALSTPSDFIEKAFEGYQYRRSLMVDGLNALGFRCSPPPAG
                     AFYAFPDISSIAADSTAFSRELLDKAHIATVPGVVFGKEGEGHLRFSYSLAPEIIEKG
                     LESLSNYLKTR"
     gene            1798..2793
                     /locus_tag="PKZ53_01875"
     CDS             1798..2793
                     /locus_tag="PKZ53_01875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ39208.1"
                     /translation="MSSDTMHPSSPVAASSSPPVNHPEWEFLYQSEGCPIFFDPASHL
                     FHSIVPLEETTSLYQEQFYEAGSAARFFSPVEVVTRFFRRRRAAYIARYVQSTGGVLD
                     IGCGNAYVLYYLREDHQVPVVIGTQVSATAQAFAREKLGIDVRLGELPVLRPDLPTFS
                     VITAWHVFEHVADYETYFKESWNMLAPGGYLILEVPNAQSWTVGVSGPGWMGWDPPHH
                     VVHFTPEGLDKLLKKQGFQPVARSTFSLEYSVFTSLQSICNRFSRMRNAFYDGLRRVG
                     GNRPPLWLILFQGMWFSLLFFPVLLINLLLAPTPWGEVLHVIAQKPAPARSSQPT"
     gene            2926..3765
                     /locus_tag="PKZ53_01880"
     CDS             2926..3765
                     /locus_tag="PKZ53_01880"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNJ39209.1"
                     /translation="MSEQTLSSQTYWREVDVACRLAQEAGKLILEYYDRTTKVSYKAG
                     DEPVTEADQAASDHILAGLHEAFPDDVLVSEEEPDNPLRLESSRLWVIDPLDGTKEFI
                     ARNGEFSVMIGFVVGERPVVGVVYQPTRDRLWWAAHDQAFLRIGGEQKSIKVAPTSDY
                     AAMRMVVSRSHRNALVDQMATRLGITQEIVSGSGGIKVGLIADGDAEVMFSPNPYTKI
                     WDMCACDAILHAAGGKVTDLQGNLLVYRGEEVFNLKGVVASNGTRHEDMIQTVAGIYT
                     PPV"
     gene            3829..4668
                     /gene="kdsA"
                     /locus_tag="PKZ53_01885"
     CDS             3829..4668
                     /gene="kdsA"
                     /locus_tag="PKZ53_01885"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNJ39210.1"
                     /translation="MNSTFFPKPPESFFLIAGPCVIESQDHALFLAEAIQKETAELGI
                     PYIFKASFDKANRSSIHSFRGPGLDAGLRVLEHIKKQTGLPVITDVHEIDQAQSAAEI
                     VDVLQIPAFLCRQTDLLVAAARTGRTVNVKKGQFLAPSDMKNVVEKIREAGGKELWLT
                     ERGASFGYNNLVVDMRSFPIMHEFGCPVVFDVTHSLQRPGGLGNATGGDSQFIEPLAR
                     AGVACGVDGLFMEVHDNPSKALSDGPNALPLQRLRPLLRQLYALDQIRRQYPLLAGSE
                     HEK"
     gene            complement(4670..>5803)
                     /locus_tag="PKZ53_01890"
     CDS             complement(4670..>5803)
                     /locus_tag="PKZ53_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNJ39211.1"
                     /translation="VASTGKPMIVNDPPASSAIVFPEMLEKYYVHGFMNIPIFTRTGS
                     VVGVLDVRRPALRGPFSETDQKLLESLANQAAVAIENAALYGELEKKKTELEEKNLII
                     VESMQELEKLYKSEQEISHTLQELNQMKTNFLIVTSHEMRTPLTVLKGYIEAISEEYL
                     GPLSKGQHQSMATCRRMIDRLVTSFNDILEMLKINEGQMTLNHTALNLRGTVSEVLAE
                     LATFVDRRHQTIEVESPEDIAIVADPEKIQLVLVNVIQNAIKFTPDEGLIRITIAAEA
                     SMAHLTVEDTGIGIEPTDLERIFEKFYTTSDPSTHTSGRYEFSARGTGLGLAIAKSYV
                     EAHGGKIWAESKGKGQGSSFHIKFPLATLPRDVEVTEDSVHST"
BASE COUNT         1387 a         1493 c         1389 g         1534 t
ORIGIN      
        1 aaatctgggt ggaactgatg ctacccaggc agggttcctc gacaacacca ttactcagtc
       61 ttctgctggc acggccaaga gcggatacct gctcacctac agctcaacac cagcggcggc
      121 aggtgcagca ccaacctata cctgtcagaa caccccgatt acgcgaactg gcatcacccg
      181 caccgggaat gattcattct ttgtcaacga aacaggcgta attacccatt cgggcgaccc
      241 aagcaataac gctacttcag catctggacc aattgaataa gtagccaggt attgtctctt
      301 tcttctaaag agcatacata aaaggcagga gaattcttct cctgcctttt gtatttttag
      361 ccatcattca gctctgactt tacacctcgt caaatcttca ataacatcgg taatctgttg
      421 tgccggctac ttttaagttt atctgtaaag agcgatcaca aaagacactc cattcatacc
      481 gaattcagca cttcaatctt tactgcactc atcacgaggt tgttatgtca acaagtccat
      541 ccaaagaaac tgaaggtttt gtacgcccca cctccaaacg gttggctcac cgatttgatg
      601 aagttggctt ctcggaaatt gtcaggattc gcaatcgggt catggaactc aaggccacag
      661 gtgaaaccat cttccagttt gaaggcggcg aaccctatat gaacacccct gattttgtga
      721 aagaagccgc gacccaggcc cttcatgaaa ataaaacccg ctatgctcct tcaagtggga
      781 tcaaaccttt ggttgatgcc attaccgaaa aactccgaac gaaaaataaa attccagccc
      841 aacccgaaca cgtcatcgta ggcaatggcg ggatgcaggg actctatggt gcctttaatt
      901 ccatcctgga tccaggtgac gaagttcttt tattctctcc gcattggaca ccaacgcgtg
      961 atctgattta tatgtcaggt ggccaggtcg tcagcatcaa tacgtttgaa gctcgacaaa
     1021 ccggcatctc ccaaacgttg caggcacatc tcagcccaaa aagccgagca atcctcctca
     1081 ataccccaca aaatccaact ggaattgtct ttactcgtgc tgaaattgaa gaagtcgccg
     1141 cgtttgccca aaaccacgat ctggtggtga ttgccgatga agcctatgaa gatctggtgt
     1201 atgagcagga acacgtttca attgcttcgt tgcccggcat gtatgaacgc accatcagtg
     1261 tctatacgct ttcgaaaagt tatgccatga caggctggcg attaggatat gccattgccg
     1321 cagaaccatt tatcaccggg ctgaaaaaat ctattttgta ttcttccaac ggagtcagca
     1381 ccccaactca gtgggccgcc gtggcagcac tctcaacacc ttctgacttt atcgaaaaag
     1441 cctttgaagg atatcagtac cgccgatcat tgatggttga tggcctgaat gcacttggat
     1501 ttcgctgcag cccgcctccg gctggagcgt tttatgcgtt tccagatatt tcttcaatcg
     1561 ctgccgacag cacggctttt tcccgggaac ttttagataa agcgcatatt gctacggttc
     1621 caggtgtcgt ttttgggaaa gaaggcgaag gacatcttcg atttagttac tcgctggctc
     1681 cagaaattat tgaaaaaggg cttgaatcac tcagcaacta cctcaaaact cgctaacttc
     1741 aggaaatcca gttgcgacga atacctggtt tcagtttttc ataaaaattc tgtcgctttg
     1801 tcatccgaca ctatgcaccc atcatcacca gtggcagcct cgtcgtcacc gccagtgaat
     1861 caccctgaat gggagtttct ctatcaaagt gaaggctgcc caattttttt tgacccagcc
     1921 agccatctgt ttcattcgat tgtcccactt gaagaaacca catctttata ccaggaacag
     1981 ttttatgaag ctggttcggc ggctcgtttt ttttcgccgg tcgaagtggt aacccgcttc
     2041 tttcggcggc ggcgggcagc ctatattgcc cgctatgttc aatcaactgg cggtgtactt
     2101 gatattggct gtggaaacgc ttatgtgttg tactatcttc gtgaagatca ccaggttccg
     2161 gtggtgatcg ggacccaggt ctcggctacg gctcaggctt ttgcccgcga gaaactcggg
     2221 attgacgttc ggctcgggga actcccagtc ttgcggcctg accttcctac cttttcagtc
     2281 atcactgcct ggcatgtctt tgaacatgtg gctgattatg aaacatattt caaagaaagc
     2341 tggaatatgc tggcacctgg cggatatttg atccttgagg ttccaaatgc ccaaagctgg
     2401 acagtcgggg tttccggtcc tggttggatg gggtgggatc cgccacacca cgtggttcat
     2461 ttcacaccag aggggctcga taaactcttg aaaaaacagg gatttcaacc tgttgcccga
     2521 tctacttttt cgcttgaata cagcgttttc acgtcacttc aatctatttg caaccgattt
     2581 tcacggatgc gaaacgcatt ttatgatggg ttgcggcgcg ttggtggcaa tcgcccccca
     2641 ctgtggttga tcttgtttca gggaatgtgg ttttcacttt tgttttttcc cgttcttctc
     2701 atcaacctac tccttgcccc gacaccgtgg ggtgaagtcc tccatgtcat tgcccaaaag
     2761 ccagcccctg ccaggtcaag ccaacccacc tgacccagat accacgcctg cattttttac
     2821 ttgaccgggc gaatcactca aagtcagact gtccggcatc tgctgagttt ccaatttttc
     2881 caggatcact ggttccccca atcctgtttt tacacgagtg tcaccatgtc agaacaaact
     2941 ctttccagcc agacctattg gcgcgaagtt gatgttgcct gccgcctggc gcaggaagct
     3001 ggtaaattga tccttgaata ttatgaccgc accaccaagg tcagctataa agcaggtgat
     3061 gaaccagtca ccgaagccga tcaggcagcc agtgatcata ttctggcggg tctccacgag
     3121 gcatttcccg atgatgtctt ggtttctgaa gaagaacctg acaatccact ccgattagaa
     3181 tcttcgcgac tgtgggtgat tgatccgctt gatgggacga aagaattcat cgcccgcaac
     3241 ggagaatttt ccgtcatgat tggctttgtg gttggcgagc gcccggtggt gggcgtggtg
     3301 tatcaaccca cccgtgaccg attgtggtgg gcggcccacg atcaggcgtt tttgcgaatt
     3361 ggtggcgaac aaaaatctat caaggtggca cccacctctg actatgccgc aatgcgaatg
     3421 gttgtgagtc ggtcacatcg caatgcgctg gttgatcaaa tggcaacccg gctgggaatt
     3481 acccaggaaa ttgtctctgg cagcggcggg atcaaagttg gcctgattgc ggatggtgat
     3541 gccgaagtta tgtttagccc caacccatat acaaaaatct gggatatgtg tgcctgcgat
     3601 gccattttgc acgcggctgg cggcaaagtg acggatctac aagggaattt attggtttat
     3661 cgaggcgaag aggtttttaa tttgaaaggg gtggttgcca gcaacggcac tcgccacgag
     3721 gatatgattc agacggtggc tgggatttac actccgcctg tgtaagggtc tgcatcaatg
     3781 tcactgattt gatgaggcaa cacctggttc ttcggtatct ttccatttat gaacagtact
     3841 ttttttccca aaccacctga atcatttttc ctgattgccg gtccctgtgt gattgaatct
     3901 caggaccatg ccttatttct ggctgaagcg attcaaaaag agacggctga actgggcatt
     3961 ccctacattt tcaaagcttc gtttgataaa gccaaccggt catccattca ttcatttcga
     4021 ggtccaggcc tggatgccgg gttgcgggtt ttagaacaca ttaaaaaaca gaccgggttg
     4081 cccgtcatta cagatgtcca tgaaattgat caggcccaga gcgcggctga aatcgttgac
     4141 gtcctgcaaa tcccagcctt tttgtgccgc cagaccgatc ttttggtggc tgccgcacgc
     4201 actggtcgaa cggtaaacgt caaaaaaggt cagtttttgg cccccagtga tatgaaaaat
     4261 gtggttgaaa aaatccgtga agctggtgga aaagaactct ggttgaccga gcgcggagca
     4321 tcctttggct acaataacct ggtggttgat atgcggtcat ttccgatcat gcacgagttt
     4381 ggctgcccgg tggtctttga cgtcacccac agcctgcaac gcccaggagg tctcggaaat
     4441 gcaactggcg gagattcaca gtttattgag cctctggctc gggccggtgt cgcctgcgga
     4501 gttgatggac tgtttatgga agttcatgac aacccgtcga aagcactgag tgatggccca
     4561 aatgccttac cgttacagcg gcttcgcccc ctcctacgcc aactctatgc cctggatcaa
     4621 attcggagac aatatcccct tctggctggc agtgagcacg aaaagtaagt caggttgaat
     4681 gaacgctatc ttccgtcact tcaacatcac gcggcagcgt ggccagtggg aatttgatgt
     4741 gaaagctgct cccctgccct ttacctttgg attcagccca gattttgcca ccgtgggctt
     4801 cgacataact tttggcaatt gccaacccaa gcccggtgcc acgtgccgaa aattcatagc
     4861 ggcctgaagt atgcgttgac gggtcagagg tggtgtaaaa cttctcgaaa atccgttcca
     4921 gatcggttgg ctcaattcca atcccggtat cttcaactgt caggtgggcc atactggctt
     4981 cagccgcgat agtaatgcga atcaaacctt cgtcaggcgt aaatttgatt gcgttttgaa
     5041 tcacgttgac caggaccagt tgaatttttt ccggatcagc cacaatggca atatcctctg
     5101 gggactcgac ctcaatagtc tggtgacggc ggtcaacaaa ggtggccagt tcggccagga
     5161 cttcagacac cgttccacgc agattcaatg ccgtgtggtt gagcgtcatc tggccttcat
     5221 tgattttgag catttccaga atatcgttaa agcttgtcac cagccggtca atcatccgcc
     5281 ggcaggttgc cattgactgg tgctgtccct tgctgagtgg ccccaggtat tcttcagaaa
     5341 tggcttcgat ataccctttg agcaccgtga gcggggtgcg catttcgtgt gaagtcacaa
     5401 tcagaaaatt ggtcttcatc tggttgagtt cttgcagggt gtgcgaaatc tcctgctcgc
     5461 tcttatacag cttttcgagt tcctgcattg actcgacaat gatcagattt ttttcttcaa
     5521 gctcggtctt tttcttctct aactcaccgt acaaggctgc attttcaatg gccaccgcgg
     5581 cctgattcgc cagggattcc aataatttct gatcggtttc gctaaagggg ccgcggagcg
     5641 caggacgcct gacatcaagc acgccgacca cgctcccagt ccgcgtaaaa atcggaatat
     5701 tcataaaacc gtggacatag tatttttcga gcatctcagg aaaaacaatc gctgaagagg
     5761 caggcgggtc attgacaatc attggctttc cagtactggc cac
//
LOCUS       DAOQDO010000066         5796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8511_length_5796_cov_3.256053, whole genome shotgun sequence.
ACCESSION   DAOQDO010000066 DAOQDO010000000
VERSION     DAOQDO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8511_length_5796_cov_3.256053"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3471)
                     /locus_tag="PKZ53_01895"
     CDS             complement(<1..3471)
                     /locus_tag="PKZ53_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39212.1"
                     /translation="MLAYATFESNGFSFDYPAAWRLERQPGFEIAVCGPSLMRVYFGV
                     ELFPDRAQLPSTNLSPLHLLAKLHDEAPEEIYGERTQLGEIHGLAARALFIREGDPFE
                     SRLVAVRCLQGTGYARACLGVVCPVTLLEPAGDVIQQILDSLALEKNPWLKMRMLPSA
                     KTTPIDFTEEMRNAPTIFLVPPRGISTPLPPRMEETGTRALISSSSEDFGLAPPLPET
                     SDRLLTVPQPAQAILAGDLPKPAPIGKQDLSSLVSTKLLADAMLGDDDDEEEDQEDND
                     EPTSQPHVAAATVEPSSYIVGTLTGGGAGFRDGPAALASFQRPNGITADPQGNLYVAD
                     FGNHRIRRISPEGITTTLAGSTSGFRDEPGSLAMFNGPRGIVYSNGYLYVADLHNLRI
                     RRVTLNGMVKTFVGSGNRGLSDGLSLQAEFDAPRAVAADTNGNIFVADGVRIRRVTPD
                     GKVQTIAGSEAGYQDGPIHKARFETLGSISLDAAGNIYVADVGNGVIRKITRDGLVMT
                     LPVGPVAQVGSPIFEPVSVLALPDGTLYIADAEDYSIKCMFPDGSVAILAGNGRQGNH
                     DGLGGQEEGCAEFWSPTGLALAKNMLFVTDRDMHVIRSIRFPSTSTGMPVEPPVEITA
                     PVGTMGRSVTPPNNAFLSGGYQVPLTPSSGLPALQVADFQVPISREPQEYTFSTLAGS
                     EVGFRDGSGEAVRFNCPMGVCVDAAGTVYVADHFNHCIRGVTTEGTVLTLAGSLESGL
                     RDDAGTRACFKGPLDLAVGSDGALYVTDHLNYCVRLVTPKGEVRTLAGNGTQGSRDGQ
                     GRSAQFEGPKGIAVSSTGKVYVADGTLIRCIDPDGTVTTLAGSERGFRDGRGTDAAFG
                     WPYAIALDAEDNCYVADAANHAIRRVTPEGTVTTLWGGPSLKFLNFPSGIVVDLTGVI
                     YVSDTNNHRILRLDPNSDRTGVTASLIGGTKQGKRNGRGTEAELSGPRGICIAPNGDL
                     FVADSVSHRIVKIRTSVTSIATPQPGPLAVVAVEPTPTAEVNRLASGPNQPQISDSTP
                     VPIRPTSPRLPALPDEPVPTSSGNSFPTWDAPPELNSPPPVLMINQEVPSFTSDAVPR
                     SVRDLAERMERQDKRPEGSPPPVVSLGKPPSGHVQSIKERGAAPPIVISEPAPPTPVP
                     PPTIVATPLPP"
     gene            3918..5075
                     /gene="recA"
                     /locus_tag="PKZ53_01900"
     CDS             3918..5075
                     /gene="recA"
                     /locus_tag="PKZ53_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNJ39213.1"
                     /translation="MADEKVEKNRALDAAIAGLEKQFGKGVIMRLGDRKVVEIAAIST
                     TCLSLDAAIGIGGLPRGRIVEIYGPESGGKTTLALHVAAEAQRQGGTVAYIDAEHAMD
                     PAYANRLGVDTASIFISQPDSGEQALEIAEALVRSGAVDLLVIDSVAALVPRAELDGD
                     MGDSLPGLQARLMSQALRKLTAIASKTNTCMIFINQIREKIGVMFGSPETTTGGRALK
                     FYASVRLDIRRTTQIKDGETTIGSRTKVKVVKNKMAPPFKDAEFDIIYGHGISREGDI
                     LDLGVEHKLVDKSGSWFSYKGERLGQGRENAKSALAANPDMVNKIDHELRILLGLIPA
                     DATTAPAPEKPVEAAPAAKAGKVKNIEAALGIKAAEPAPAPTPATGKAAEV"
     gene            5480..>5796
                     /locus_tag="PKZ53_01905"
     CDS             5480..>5796
                     /locus_tag="PKZ53_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39214.1"
                     /translation="MYQDLALLERLLVDYVLGPDALGQYDQGDIAKRFLEPAATGLVH
                     LSTQFTTQRQEFTKSYWHETERMAYLLYYLPVNFAKAYAVLTELNHRPEVFSPSFLAQ
                     NPIR"
BASE COUNT         1324 a         1575 c         1599 g         1298 t
ORIGIN      
        1 gggtggaagc ggtgtggcca caatcgtggg tggcggcacc ggagttggag gagccggttc
       61 cgatatcaca attggtggtg cggcgcctcg ctctttaatg ctttgcacgt gcccggaagg
      121 tggcttgcca agactgacaa ctgggggcgg tgaaccctct ggacgtttgt cctgtcgttc
      181 cattcgctcg gctaaatcgc ggactgaacg gggcaccgca tctgaagtaa acgaggggac
      241 ttcctggttg atcatcaaca caggtggcgg gctattgagt tcaggtgggg cgtcccaggt
      301 tggaaagcta tttccagaag atgtgggaac tggttcatct ggcaaggctg gcaatcgggg
      361 actggttggt ctgattggca ccggtgtgct gtcactgatt tgcggctgat tggggccaga
      421 tgcaaggcga ttgacctcag cggtcggtgt tggctcgacg gccaccacgg ccagcgggcc
      481 tggctgagga gtcgcgatag aggtaaccga ggttctgatt ttgacaatgc gatgggacac
      541 cgaatcggcg acaaacagat cgccgtttgg cgcaatacag attcccctgg gaccactcag
      601 ttcagcctcg gtcccacgtc cattgcgttt tccctgcttg gtgccgccga tcaggctggc
      661 tgtaacaccg gttcgatctg aatttggatc caggcgtaaa atgcgatggt tattggtatc
      721 tgagacgtaa atgacaccgg tcagatcaac cacaatcccg cttggaaaat tcagaaattt
      781 caatgatgga ccgccccaaa gggtggtcac cgtcccctcg ggggttaccc ggcgaatggc
      841 atgattggcg gcatccgcca catagcaatt gtcttccgcg tcaagtgcaa tcgcataggg
      901 ccagccaaag gccgcatcgg tgccacgccc atctcgaaag ccgcgttcgc tgcctgccag
      961 ggtcgtgacc gttccatcag gatcaataca gcgaatgagt gtcccatcag ccacatagac
     1021 tttgcccgtc gaactcactg caatgccttt tgggccttca aactgagccg agcgaccctg
     1081 gccatcacgt gacccctggg ttccattccc agccagcgtt cgcacttcac ctttgggagt
     1141 caccaaccgg acacagtaat tcagatggtc ggtgacatag agggcaccat ctgagccaac
     1201 ggccagatca agtggccctt tgaaacaggc ccgtgttcca gcgtcatcgc gcaacccgct
     1261 ttccaggcta cccgccagcg ttaaaaccgt tccttcagtg gtcacgcctc ggatacagtg
     1321 gttaaagtgg tcagcgacat agacggttcc agcggcatca acacaaactc ccatggggca
     1381 attaaatcgg acggcttcac cgctcccatc gcgaaaccca acttcactgc cagccagtgt
     1441 ggagaacgta tattcttgtg gttcccggct gattgggacc tggaagtcag cgacctgaag
     1501 tgccgggagc ccgctggatg gtgtgagcgg cacctggtac ccgcctgaaa gaaatgcgtt
     1561 gtttggaggt gtcacgctcc gacccattgt tccaacgggt gccgtgattt caactggtgg
     1621 ctcgacgggc atcccggttg aggtagaggg aaatctgatt gaccgaatga cgtgcatatc
     1681 ccggtcagtg acaaacagca tattttttgc cagggcaagc ccggtcggtg accagaattc
     1741 agcgcatcct tcttcctgtc cgccgagtcc atcgtgattg ccttgacgac cgttgccggc
     1801 cagtatcgcg acgcttccat caggaaacat acacttgatg gagtaatcct cggcatcggc
     1861 gatatagagt gtgccatcgg gaagggccag gaccgaaacc ggctcaaaga ttggcgaccc
     1921 cacctgggct accggtccaa ccgggagtgt catgaccagc ccatcgcggg taattttgcg
     1981 aataacaccg tttccaacat ccgcgacata gatgttccca gccgcatcca gggaaatgct
     2041 gcccagcgtt tcaaagcggg ctttgtggat cgggccatct tgatagccag cctcactccc
     2101 ggcaatggtc tggactttgc catcgggcgt gactcgacgt atgcggaccc catctgccac
     2161 aaaaatattg ccattggtat cggcggcgac agcccgtggg gcatcaaatt ctgcctgcag
     2221 gctcaggccg tcgctcaatc cacgatttcc gcttccgaca aaagttttca ccatgccgtt
     2281 gagcgttacc cggcggatgc gcaggttgtg caggtcggcg acatagagat agccattgga
     2341 gtacacaatc ccgcgtggtc cattaaacat ggccagcgac cctggttcgt ctcgaaaacc
     2401 agaggtgctc ccggccagcg tggtggtaat gccttctggg gaaatccggc gaatgcgatg
     2461 gttcccaaaa tcagccacat acaaattgcc ttgcgggtca gccgtaatgc cattggggcg
     2521 ttgaaatgaa gccagggcgg ctgggccatc tcgaaacccg gcgccaccac ccgtgagtgt
     2581 cccaacaata taggatgatg gctcaaccgt tgccgccgca acgtgcggct ggctggtggg
     2641 ttcatcgttg tcttcctggt cttcctcttc gtcatcatca tcgccaagca tggcatctgc
     2701 caggagtttg gttgagacca gcgaggacag gtcctgtttt ccaattgggg ctggtttggg
     2761 caggtcaccg gccaggattg cttgtgccgg ctggggaacc gtgaggagac gatctgaggt
     2821 ttccggcaat ggcggcgcca gcccaaaatc ttcagaggag gatgaaatca gggcacgggt
     2881 cccggtctct tccatacggg gcggcagtgg ggttgaaatc ccacgtggcg gaaccaggaa
     2941 aatggttggg gcgttccgca tttcttcagt aaagtcaatc ggagtcgttt tggccgaggg
     3001 gagcatccgc attttgagcc aggggttttt ttccagggcc aggctatcca ggatttgttg
     3061 aatgacgtcc ccggcgggtt caagcagggt caccggacac accacgccca aacaggcccg
     3121 ggcatagcca gtcccttgca ggcagcgtac ggccacgagt cgtgattcaa atgggtcgcc
     3181 ttcacggata aacagggcgc gggccgccag gccgtgaatt tcaccaagct gggttcgttc
     3241 cccgtaaatt tcttcaggtg cttcgtcgtg aagcttggcc agaagatgga ggggagacag
     3301 attggttgac ggaagctgag cccggtctgg aaacagttcc acaccaaaat acacccgcat
     3361 caggcttggc ccgcacacgg cgatttcaaa accaggctgg cgttcgagtc gccaggcagc
     3421 cgggtagtca aaggaaaagc cgtttgattc gaaagtggca taagcaagca tggaatcctc
     3481 gtggctggta acgagtgatc agtgccggtc tggaagtcgt ttggaacccc agtgcgactg
     3541 ttcgctctta tccggtttgt tctttttttt caatgatttc cagttgaatc aaggtacttt
     3601 cgatggtata gtctggcggc ggttgggagg tgaatcactg acactcaggg ctgaaagaat
     3661 acccccacca gatggagaaa atccacgtct gaagatggtg tgaggtgcag tggtttcaaa
     3721 ttttgccgtt caggcgtgct cactgttttt ttccttattt caacaaagag cacttttctt
     3781 ttctatcaca atgtaataaa atattgaacc tggtggcaga tcaccacgta agatgcgcca
     3841 ccagcaacct tagcattatt cgagagcaaa cgccaaactc tgtaacctta acttttagaa
     3901 cccgttagga aaacaccatg gcagacgaaa aagttgaaaa aaatcgagca ctggacgccg
     3961 cgattgcggg attggaaaaa caatttggca aaggcgtgat tatgcgcctt ggagatcgca
     4021 aagtcgttga aatcgccgcg atttcaacca cctgtttaag cctggacgcc gccattggca
     4081 ttggtgggct tcctcgtggc cgcatcgttg aaatttatgg gccagaatcg ggcgggaaga
     4141 caacgctcgc gcttcatgtt gccgccgaag cccagcgaca aggcggaacc gtggcctaca
     4201 ttgatgccga acacgcgatg gatccagcct atgccaaccg actgggggtt gataccgcca
     4261 gtattttcat ttcccagcca gattcaggtg aacaggcgct ggaaattgcc gaagccctgg
     4321 tccgttccgg agcagttgat ttattggtga ttgactccgt ggccgcgctg gtaccgcgag
     4381 ctgaactgga tggtgatatg ggagattccc tgccggggtt gcaggctcgc ctgatgtcgc
     4441 aggcgctgcg caaactcacg gcgatagctt caaaaaccaa tacctgtatg atctttatca
     4501 accagatccg ggaaaagatc ggcgtgatgt ttggttcgcc ggaaaccacc actggcggtc
     4561 gggccctgaa attttatgcc tcggtccggc ttgatatccg ccgaaccacc caaattaaag
     4621 atggtgaaac cacaattggg agtcggacca aagtcaaggt tgtcaaaaac aaaatggcgc
     4681 cgccgtttaa agacgccgag tttgacatta tctatggcca cggtatttct cgcgaaggcg
     4741 acattctcga ccttggcgta gagcacaaac tggttgacaa gagcggctcc tggttttcct
     4801 acaaaggcga gcgtcttggc cagggacgtg aaaatgctaa atcagccctg gcggccaatc
     4861 ctgatatggt caacaagatt gatcacgagt tgcgaatcct tttgggattg attccagccg
     4921 atgcgacaac ggcaccggca cctgaaaaac cggttgaagc ggcacccgcc gccaaagctg
     4981 gcaaagtcaa aaacatagag gctgcattag gaatcaaagc cgccgaacca gccccagccc
     5041 caaccccagc cacgggcaaa gcggcggaag tctaaagaca tcgggctgaa aaagtcgggc
     5101 tgaagacaac gggtttcata ccttgagccc gctgtcttca gcccgttttc ttcaacccca
     5161 ggcccattgt cttccgctga tgcaaccagg gaattgcctg ccaccacact gctcccccag
     5221 gctgtgtacc ttcgacaccc tactctccga tttgccctca cttcctgagt ctctgccatt
     5281 tgctgccccg gctgaaccct ctgaatttct aaaccatccc aaaaagatcc atcctggaaa
     5341 aatttacaga aagaaaaatc ttgctggcca gtcatcaaga tcttttcact ttttgtcaaa
     5401 cttggagttg gttttgctga gcggtccgca gtagagtgct ttgttcatca ttatcaatcg
     5461 acaaaggtac tcattcaaaa tgtatcagga tttagctctt ctcgaacggt tgctggttga
     5521 ctatgtcctc ggccccgatg ccctcggtca atatgaccag ggagacattg ccaaacggtt
     5581 tctagaaccg gcggcgactg gactggttca tctttcaacc cagttcacca cccagcgcca
     5641 ggaattcacc aaaagttact ggcatgaaac tgagcggatg gcctatttgc tctattacct
     5701 gccggtgaat ttcgccaaag cctacgccgt actgaccgaa ctcaaccacc gacccgaagt
     5761 gttttcgcct tcctttctgg ctcagaatcc gattcg
//
LOCUS       DAOQDO010000067         5778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8557_length_5778_cov_3.057487, whole genome shotgun sequence.
ACCESSION   DAOQDO010000067 DAOQDO010000000
VERSION     DAOQDO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8557_length_5778_cov_3.057487"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKZ53_01910"
     CDS             complement(<1..677)
                     /locus_tag="PKZ53_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39215.1"
                     /translation="MADKILVVDDDPSLRKMLTTVLSKHYQVEEAASGEDALKIAAQG
                     DVRLVLLDVMMPGMSGLETCLRLRRNPRSCHATIVMLTARKQEEDVVEGLKVGADDYL
                     TKPFKFSELIARIDSHLRRQWRELQASPLTGLPGNLQIQAIIKTNIKAGIRFAVIHAD
                     LNNFKAYNDRYGFTAGDEVLNFTANVLTSAVNALGVAETDFVGHIGGDDFIIVTAPER
                     SESLCQT"
     gene            complement(788..2233)
                     /locus_tag="PKZ53_01915"
     CDS             complement(788..2233)
                     /locus_tag="PKZ53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNJ39216.1"
                     /translation="MPIEDLREFIRALEKQGELKRIQTEVSCELEITEITDRVSKAGG
                     PALLFENVTGYRTPVLINALGSERRMLIALGAESYDEIAGRLTELIDFKSPEGLMEKI
                     KLLPKLAELGSFFPKRVKSGPCQEVVLLEDEFSLLKLPILKCWPEDGGRFITFPLVFT
                     NSPLNGKRNVGMYRMQVYDGQTTGMHWQMHKHGAAHFREHVRTGTRMPVSVAIGGDPL
                     LTFAAIFPLPDDVDEMMLAGFLRSKPVPMVKCITNDIEVPANAEIVLEGYVDPHEQRV
                     EGPFGDHTGFYTEAKDFPVFHVTCVTHRRHPIYQTTIVGRPPMEDCYMGEAVGRIAMP
                     VLKRQFPEVVDMHLPFEGVFHNLMIVSIKKAFPGHARKIMNSVWGTGQMMFTKCIIVV
                     DEDVPVRDPQYVAWYVLNNIDPERDIQFTMGPIDELDHSSRMIAYGSKMGIDATRKWP
                     SEGFHRDWPKENRMSDDIVKLVDAKWKTLFK"
     gene            complement(2288..>5778)
                     /locus_tag="PKZ53_01920"
     CDS             complement(2288..>5778)
                     /locus_tag="PKZ53_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39217.1"
                     /translation="QHTDEQAALRKSVSELEQQTEKFRQQCTELEERYARRNAEFHLQ
                     RDELNRLQLLVAPYRELQVKYLDLEQREKLLEERFQGLRATFEKVEFERTKFEDELRE
                     TRDRVVGLQKEVSDERRQHERELEAERQLHQEQVMKLMSEIADLEETKRTLNELSTAY
                     ARLRSVWDDAQNDLAQARAAMDTTLNDVRNAEQSTRMLRVEAERLASDRARLVTENQS
                     LQAELETLKAIPPVDVAPYEARIVELEATLLTAQTRHTEFQQTFEDLEAKMADRSAQE
                     ATLHDQIHQLSERITQLSAQSEAHRVSTEALQQELERLQAIPPVDVSPYEVRISHLEQ
                     HLSVHETEKLARQDEITSLLAEVENHKLEKEQLREALRDLTSQFEALTTETEARELLL
                     HTIQSELDAVRAEPPVDVAPYEDRIQNLEASLAEQAGHLTDSQAEIDRLQKEQAGQAA
                     AREQEREELARLHVQVNSQFTQISELYSQIEADEAERATLRAAVKTLEDRIHESHLRS
                     QRLQMMHDALGSAHDEVKQLLGAAVQDRHYLERESEQLQAANSRLEAQAVQLTQLTTR
                     LTVENEDLQLQLAELKQQSTRLTAQNEQLQTQVLEIAALYDQQRKQYEVFQTRFLQRL
                     KLQEAERADWQRLLDKAAGTDLTLHETLQELEQVKAESARLQVHTEHLEAQKSELITQ
                     IDEVNTRYLKQRDRFNTVLPRLLERLRLLDAERTDWKRLIDHAAATETDLREAIKARE
                     AADQAHEAAAQTELELELQDLAQTLAQTRAQSDELVHTLQARLDQSRMEQETLHTEIE
                     QLQGRLTGELDQTVLLRAQKGQLEAEVAELQAKLEAALGEQAVLQTEIERLQGRLTGE
                     LDQSVLLQSQQAQLEAEVAELQTKLEASVDEQAVLQTEVERLQGRLTGELDQAVFLTA
                     ENAQLQTSVDELQTQLEESQQSRQVLETQVSATAQALAETEAARLELSRQFDAQMADY
                     QNVLDATQQEVHRIREHLEAVLKEKQALLERSQSPAPELDPLFQSVETLIPISSEPTE
                     TLSAPVVVSGPLRILLASDAPDGAAHLQSLLAEQGHSISFAPDGETALSAIFLDVPDL
                     VIANLESPYFDTADLLQSLRRNPGWRKLPVILLYSDVPEAQRALLPARNEQTQLLFQD
                     ALTEDTIRQALTIAQK"
BASE COUNT         1219 a         1561 c         1536 g         1462 t
ORIGIN      
        1 atggtttggc agagagactc gctgcgttcc ggggcagtca caatgatgaa atcatcccca
       61 ccgatatgcc ccacaaaatc ggtttcagcc accccgagcg cgttgacagc actggtcaac
      121 acgttggccg taaagtttaa aacttcatcc ccggcggtaa acccatagcg gtcattatag
      181 gctttgaagt tgttgagatc ggcgtgaatg acagcaaagc ggatcccagc tttgatgttg
      241 gttttgatga tggcctgaat ctgcagattc ccgggcaaac cagttaaggg actggcctgc
      301 aattcacgcc actgacggcg caggtgtgaa tcaatgcggg caattaattc agagaattta
      361 aacggtttcg tcaaataatc atcagccccg acctttaacc cttcgacgac atcttcctct
      421 tgtttacgcg ccgtcaacat cacaatcgtg gcatggcaac tgcgtggatt ccggcgtaac
      481 cgcagacagg tctcaagtcc gctcatcccc ggcatcatca cgtccagcaa gaccagccgg
      541 acgtcacctt gagccgcaat tttgagcgcg tcttcgccag acgcggcttc ttccacctga
      601 tagtgcttgc tcagcaccgt ggtcagcatc ttgcgcaggc ttggatcgtc atctacaacc
      661 agaattttat cagccataaa ttgagggggt ggggttagag gtcagagatt ggaaaaacaa
      721 cgacacaaag aacaaaagcg ctgataagga ctgcaattca agaaccctga accctgaacc
      781 ctgaacccta tttgaacagc gttttccact tggcatcaac taacttgaca atatcatcgg
      841 acattcggtt ttccttcggc cagtcgcggt gaaacccttc gctcggccat ttacgggtgg
      901 catcaatccc cattttagaa ccatatgcga tcattcgaga agagtgatcc agttcgtcaa
      961 ttggacccat agtaaactga atgtctcgtt cgggatcaat gttgttgaga acataccacg
     1021 caacgtactg cgggtcccga accggcacat cttcgtcaac cacaatgata catttggtaa
     1081 acatcatctg cccggtgccc caaaccgaat tcataatttt tcgggcatga ccgggaaaag
     1141 cttttttgat cgagacgatc atcaggttgt ggaatacacc ttcaaacggc aggtgcatat
     1201 ccaccacttc tggaaactgg cgtttgagaa ccggcatggc aatgcgcccg acggcttcac
     1261 ccatataaca gtcttccatc ggtggccgtc cgacgatggt ggtctggtaa atcggatggc
     1321 ggcggtgggt aacacaggtc acgtgaaaca ctggaaagtc tttggcttcg gtataaaacc
     1381 cggtgtggtc gccaaacggg ccttcaacac gctgttcatg cggatcaacg tacccttcga
     1441 gcacaatttc tgcattggcg gggacttcga tgtcattggt aatacatttg accatcggca
     1501 ctggctttga gcgaagaaac ccggccagca tcatttcatc cacatcatct ggcaagggga
     1561 aaatcgcggc aaacgtcagc agcgggtcgc caccaatcgc cactgaaacc ggcatgcgcg
     1621 tcccggtgcg cacatgctca cggaaatggg ccgccccgtg tttgtgcatc tgccagtgca
     1681 tgccggtggt ttgaccgtca tagacctgca tgcgatacat cccgacattg cgcttgccat
     1741 tgagcgggct attggtaaac accagcggga aggtaataaa ccgaccaccg tcttcgggcc
     1801 agcatttcag aataggcaat ttcaggagtg aaaactcatc ttcaagcaga accacttcct
     1861 gacaggggcc acttttgact cgtttgggga aaaaactgcc cagttcagct aacttcggca
     1921 gcagtttgat tttctccatc agtccttcag gtgatttgaa atcaatcagt tcggtcagac
     1981 gaccggcaat ttcgtcgtag gattcagcgc caagtgcaat cagcatccgg cgctcactcc
     2041 ccagcgcatt gatcaggacc ggggtgcggt atccagtcac attttcaaac aacagggctg
     2101 gaccaccggc ttttgaaacc cggtcggtaa tttcggtaat ttcaagctca cacgaaacct
     2161 cggtctggat ccgcttgagt tcgccctgct tttcgagggc tctgataaat tctcgtaaat
     2221 cttcaattgg cataggtttc aacaaaagat ggaaagctac gtcgagccgt agcactccaa
     2281 aacggcttca tttttgggca attgtgaggg cctgacggat ggtatcttcg gtgagagcat
     2341 cctggaaaag caattgggtc tgctcgtttc gtgccggaag cagggcccgt tgggcttctg
     2401 gaacatcgct atagagcaag atcaccggca gttttcgcca gcctgggttg cggcgcagac
     2461 tttgcaacag gtccgcagtg tcaaagtacg gactctccag attggcaatc accaggtcag
     2521 gtacatccag gaaaatcgcc gaaagggcgg tctctccgtc tggggcaaac gaaattgaat
     2581 gcccttgttc agccaggagc gattgcagat gggcggctcc atctggggca tcgctggcca
     2641 gcaaaattcg aagcggtccc gaaacaacca cgggtgccga caaggtttcg gttggttcgc
     2701 ttgatatcgg aatcagggtt tcaaccgatt ggaaaagcgg atcaagttca ggtgcaggtg
     2761 actggcttcg ttccagcaac gcctgctttt cctttaaaac cgcttctaaa tgttcgcgaa
     2821 tccgatgaac ttcctgctgt gtggcatcca gcacgttttg atagtccgcc atttgggcat
     2881 caaactggcg gctcaattcc agtcgggcgg cctcggtttc agccagggcc tgagccgtcg
     2941 ccgatacctg ggtttccaag acctgccgcg actgttgaga ttcctcaagc tgggtttgca
     3001 attcatccac ggatgtctgc aactgcgcat tttcagccgt tagaaacacg gcctgatcca
     3061 gttcgccggt caaccgtccc tgcaaccgtt cgacttcggt ttgcaaaact gcctgttcgt
     3121 caacggatgc ttcgagtttg gtttggagtt cagctacttc cgcttcgagc tgagcctgtt
     3181 gagattggag caaaactgac tgatccagct cgcccgtcag ccgcccctgc aaccgttcga
     3241 tttcggtttg caaaaccgcc tgttcgccca gtgcggcctc cagtttggcc tggagttcgg
     3301 ccacctccgc ttcaagctga cctttctggg cacggagcaa caccgtctgg tccagttcac
     3361 cagtcaagcg tccctgcaac tgttcaattt cagtgtgcag cgtttcctgt tccatccgag
     3421 actgatccaa tctggcctgc aaggtgtgga ccagttcatc tgactgggcc cgggtttggg
     3481 ccagtgtttg ggctaaatcc tggagttcga gttcaagttc ggtttgggcc gctgcttcgt
     3541 gggcctgatc ggcggcttcc ctggccttga tcgcctcccg gagatcggtc tcagtggcag
     3601 ccgcgtgatc aatcagacgt ttccaatccg ttcgctctgc gtcgagcagt cgaagccgtt
     3661 ccaaaagacg cggaagaacc gtgttaaacc gatctctttg cttgagatag cgggtattta
     3721 cttcgtcaat ctgagtaatc agttcgctct tttgggcttc caggtgttca gtgtgaacct
     3781 gcaaccgggc tgattcggct ttgacctgtt ccagttcctg caatgtctcg tgcaacgtca
     3841 agtcagtgcc agccgctttg tcgagcaggc gttgccagtc ggcgcgctcg gcttcctgta
     3901 atttcagccg ttgcaaaaac cgggtctgaa agacttcata ttgctttcgt tgctgatcat
     3961 agagtgccgc aatttccaga acctgggttt gcagttgttc attttgggcc gttaaccggg
     4021 ttgactgctg cttgagttca gccaattgaa gctgtaaatc ctcgttttcg acggtcaacc
     4081 gggtggtcaa ctgggtcaat tgcaccgcct gagcttccag acggctattg gcggcttgca
     4141 actgttcaga ttcacgttcc agatagtggc gatcctgaac cgcagcaccc agcaactgtt
     4201 tgacttcatc atgggctgac cccagcgcgt catgcatcat ttgcagccgc tgggaccgca
     4261 gatgtgattc gtggatgcgg tcttccaggg ttttgacagc cgcgcggagc gtggcccgct
     4321 cggcctcatc agcttcaatc tggctataga gttctgaaat ttgggtaaac tggctgttga
     4381 cctgcacgtg cagccgcgcc agttcctcac gttcctgctc acgggctgcg gcctgcccgg
     4441 cctgttcttt ttggaggcgg tcaatttcgg cttgtgagtc ggtcaggtgc ccggcctgct
     4501 cggccagcga tgcttccagg ttctgaatcc ggtcttcata cggcgcgaca tcaacggggg
     4561 gctcggctcg cacggcatcc agctctgatt gaattgtgtg caaaagcaat tcacgcgctt
     4621 cggtctcggt ggtcaaggct tcaaattgac tggtgaggtc ccgcaatgcc tcgcggagct
     4681 gctctttttc aagtttgtgg ttttcaacct ctgccagcaa actggtgatc tcgtcctggc
     4741 gagcaagttt ttcagtttca tggacgctga gatgttgttc aaggtgtgaa atccgcactt
     4801 catatgggct gacatcaacc ggtggaatgg cctgcaggcg ctccagctct tgctgcaggg
     4861 cctcggttga cacccgatgg gcttcagatt gggcgcttaa ctgggtgatg cgctcagaca
     4921 actggtggat ctggtcgtgg agggttgctt cctgggctga acggtctgcc atttttgctt
     4981 caaggtcctc aaaggtttgc tgaaattcag tgtgtcgggt ctgtgcagtc agtaaggtcg
     5041 cttctagttc gacaatccgg gcttcatacg gggcaacatc tacaggtgga atggctttga
     5101 gtgtctccag ttcggcctgc agtgactgat tctcggtcac aagccgggct cgatccgatg
     5161 ccagtcgttc ggcttcaacc cgcagcatcc gggtggattg ctcggcattg cgcacatcgt
     5221 tgagtgtggt atccatggcc gcccgggcct gcgccagatc attttgggca tcatcccaca
     5281 ccgaacgcag ccgggcatag gccgtgctca gctcattgag tgtccgtttg gtctcttcca
     5341 ggtcagcaat ttcagacatc agcttcatga cctgttcttg atgaagttgt cgctcggctt
     5401 cgagttcgcg ttcgtgctga cggcgctcgt cgctcacttc cttttgcaac cccaccaccc
     5461 ggtcacgggt ttcacggagt tcatcttcaa atttggttcg ttcaaattcg accttttcaa
     5521 aagttgcgcg caacccctgg aagcgttctt ccagcaattt ttcacgttgt tcgaggtcaa
     5581 ggtatttgac ctgaagttca cgatacgggg cgaccagcaa ttgcaggcgg ttgagttcat
     5641 cccgctgcag gtgaaactcg gcatttcgcc gggcatagcg ttcttcgagt tcagtgcatt
     5701 gctggcggaa tttttcagtc tgctgttcca gttcggaaac agattttcgg agggcggcct
     5761 gctcatcagt gtgttggt
//
LOCUS       DAOQDO010000068         5767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8586_length_5767_cov_5.438200, whole genome shotgun sequence.
ACCESSION   DAOQDO010000068 DAOQDO010000000
VERSION     DAOQDO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8586_length_5767_cov_5.438200"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKZ53_01925"
     CDS             <1..181
                     /locus_tag="PKZ53_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39218.1"
                     /translation="DGARLAGGQRGFARATTGCGQPQWVSRTARTPLGRGPGGEEEPS
                     NLFPVVAPKASQPTG"
     gene            complement(228..521)
                     /locus_tag="PKZ53_01930"
     CDS             complement(228..521)
                     /locus_tag="PKZ53_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39219.1"
                     /translation="MTLNDGKIGEDKETRGQGDEGYGDKETRGQGDKENEALTGCTGL
                     LPASADWYDSEANEQVKNNPEIKNNETRKRRRIEFISFKVSCQSFVVKNQPGE"
     gene            511..>5767
                     /locus_tag="PKZ53_01935"
     CDS             511..>5767
                     /locus_tag="PKZ53_01935"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39220.1"
                     /translation="MRVKQAPTSGQALSRRAPSINGRLEGSIQQLTPENVTLNGGATI
                     TEKLYVPGTPTVRRNGNPNFGGVIEGSGSAIPTNYQVTLNGNATLGNLVNRTNPIALT
                     TVAAPPTPTGTRDVSLNNPNDQIGDFATLRNLTLNGNAGTRSVPPGTYGNFTVNGSSV
                     LIFGIANATEPAVYSLQGLTLNGGTQLQIVGPVTLTLRNGTNINGSIGNAANPQWLTL
                     NVSNGGVTLNGGSQMFGKVNAPAGSVTVNSRLTGNIICDRLTVNGGGVVSILAQAPQD
                     TTAPVITITEPAEGLITKTSPVTVSGTVTDASAVTVTVNSQPVTLSGAEFSTSITLAE
                     GSNTITVLATDTFGNVGMVLRNVTLDTIAPVLTITSPQDGSVTTETQLTITGTVTDAT
                     TTTVTVNNVAATRNGNEFSAVVSLVEGPNIFVVRAQDAAGNQTETTRTVTLQVPHDTT
                     PPVITLIEPAQGLITNVSPVTVSGTVTDVSAITVTVNNQPVTLSGTQFSTSVTLAEGS
                     NTITVSATDAFGNASSSSRTVVLDTTIPVLTITFPQDQATTTEEQITVTGTITDTNTI
                     TVTVNDIAATLTGTEFSATVPLEIGANTLTVKVVDAAGNIKETTQTITRQNTEPLQLS
                     VTEPAEGSLLNSNPVLFAGNVTGSNGSATTVTVLDKTFSVSPEGTFGGYVDLRQGPNV
                     VPIVATDATGRRSEVIRNVTLDAFAPVFQNVTPADGSLIAANSVVVRGTIEDDQPVTL
                     FINGTQTDLVSGAFVSAVIPIQEQIENSIRLVAIDEAGNEATLYLKLIGKDLTPPVPP
                     TIFPVVSPTRLDTQTIAGRAEPGTAIAINGGMSPVSVRASSGGGFFLASIPLAEGFNQ
                     LQAVATDAAGNVSTTASFAMTRNALLPPPPPGQAWQINVGTGDAQHGLVKMELPRPLV
                     VYVSDTAGSPVAGAPVTFSVLQGSGQFADGQHTLATTTNSQGYATARYVAGDLPGTDV
                     VRADFAGNTAVPTQFFATIYRPIPGGTTQFLGLVLDQNLRALPNVLVRLAGQQTRTDD
                     KGYFQFQNVPSGPHQLLELIGRDQIQLPGRWPNISYDVDILPGIDNRIDRPLFLPKVN
                     EGIEFPLDQNNVVTEDTTYELPVVGGQPPIRVTARAGTQINFPPDVSDRRFSVTRIAA
                     NRIPMALENGLTTNLYVSVQPSGAIFDPPLEVEFPNLDGLAPNSPVLLMSFDHDAGRY
                     VQAGTGTVDETGQIVRGNPGSGIRVGAWHAFPPTAPPPDGWLQAANPSSVSIEDPVPC
                     YPSPLRYSCTPPFGTNLVTCDDDLCWMRGVGVGNFETVCQQVFTINLNDDNYITDANL
                     LAIRYGTWGAANDQGQMIGVLIESIANPRRSMIKLYRFKSDGAQHDVKTILLPELPPP
                     SARTTKFEISRDFRFVNFYGPANNNDQFVVFTTEDQRKGDDFERHTSKVRIYTSGGAL
                     VQCVDNTVPTQSSTRDEIDIVSFVSDCDGNPDSNNGINLADCDRTEKIGTRIGDFRTV
                     MANNREYASIIPSNLRIDKKFAATQVYFIRGLEVFNPNTPMLNLKRVGFRNAENGGDG
                     KPVGGDTIERANVATGDLTFPDSTTKPGLIVLSKSFLLVFDPIAGELVRTRVVSDNGI
                     RGPKGPNGEPPPTEPISANDFTPLFDMPVAYSGNIIGPKVVSGKFTAPTDSNGVEIFN
                     TPQGRRYGMMRLFNKTLFVAAHSLPNVINNTRPSCDGTRSDIVGPFNSIYTSYSSFFA
                     GGLFLPGGWSGKPFPEELGVCENQTL"
BASE COUNT         1392 a         1586 c         1465 g         1324 t
ORIGIN      
        1 ggatggagca aggttagccg gtggtcagcg tggctttgca cgcgccacca ccggatgtgg
       61 tcaaccccaa tgggtctccc gcacggcccg caccccgttg gggcgcggac cgggcgggga
      121 ggaagaaccc tccaatctgt ttccggtggt agctcccaaa gcgtcgcaac cgaccggcta
      181 agcttgctcc atcctttcag gatgagaacc aaaactgctt caacgcttca ttcgcctggc
      241 tgatttttga cgacgaacga ctgacaactg actttgaagg atatgaattc tatgcgtcgc
      301 ctttttctgg tttcattgtt tttgatttcg ggattgtttt ttacctgttc attggcttca
      361 ctgtcgtacc aatccgctga agctggaagc aatccggtac atccggtcaa tgcttcattc
      421 tccttgtccc cttgtcccct tgtctccttg tctccgtatc cctcatcccc ctgtcctctt
      481 gtctccttgt cttccccgat tttcccatca ttgagggtca agcaagcacc gaccagtggg
      541 caggctcttt cccggcgggc accttcaatc aatggccgac tggaaggcag catccagcaa
      601 ttgacaccag aaaatgtgac cttaaacggt ggagcgacga ttactgaaaa gctctatgtc
      661 ccagggacac caacagttcg gcgcaatggc aacccgaatt tcggtggtgt gattgaaggc
      721 agcggaagtg ccattccaac caattatcaa gtcacgctca atggcaatgc cacgctggga
      781 aatctggtca atcggaccaa tcctattgcg ttgacaacag ttgcagcacc tccgacacca
      841 accggaacac gggatgtcag tttgaacaat cccaatgacc agatcggaga ctttgcgacg
      901 ctccgcaatt taaccttgaa cggcaatgcc ggaacacgtt cggtgccacc aggtacctat
      961 ggaaatttca cggtcaatgg gagcagtgtc ttgatttttg gcatcgcgaa cgcgactgaa
     1021 ccagcggtct actcactcca aggattgacg ctcaacggag gcacccagct tcaaatcgtg
     1081 ggaccagtca cgttgacgct tcggaatgga acgaacatca acggcagcat cggcaatgca
     1141 gccaatccac agtggctcac actcaacgtt tccaacggag gcgtcaccct caacggcggc
     1201 agccagatgt tcggaaaagt gaacgctccc gctggaagcg tcaccgtcaa cagccgcttg
     1261 actggaaata tcatctgtga ccgattgact gtcaacggag gcggagtcgt ctccatcctg
     1321 gcgcaagctc cacaggacac caccgctccg gtcatcacaa ttactgaacc tgctgaaggg
     1381 ttgatcacca aaacctctcc agtcacggtt tccgggacgg tgactgacgc cagtgcagtg
     1441 accgtgacgg tcaatagcca acccgtaacc ctttccggag cagaatttag caccagcatc
     1501 acgctggctg aaggttcaaa cacaatcact gttctggcta ccgacacctt tggaaacgta
     1561 ggaatggttt tgcgcaatgt caccctcgac accatcgctc cagttctgac catcacttct
     1621 ccccaagacg gatccgtcac gaccgaaacc caactcacca tcactggaac cgtgactgat
     1681 gccaccacaa ccacggtgac tgtgaacaat gtcgccgcca cccgaaacgg aaatgaattc
     1741 tccgctgtgg tttcactggt ggagggtcca aatatcttcg tggtccgagc ccaagacgcc
     1801 gccggaaacc aaactgaaac cacccgaact gtcacgcttc aggttccaca cgacaccacg
     1861 ccacctgtca tcaccctcat cgaaccagcc cagggcttga tcaccaatgt ttctccagtc
     1921 acggtttccg ggacagtgac cgatgtcagc gccatcaccg tcacggtcaa caaccagccg
     1981 gtaactctat ctgggacaca atttagcacc agcgtcacac tggctgaagg ctcaaacaca
     2041 attactgttt cagcgacaga tgcgtttgga aatgcttcaa gttcttcgcg gactgttgtg
     2101 cttgatacca caataccggt tttgaccatc acttttcctc aagaccaggc caccacgacc
     2161 gaggagcaga tcacggtgac gggaacgatt accgacacca acaccatcac ggttacagtc
     2221 aatgacattg ctgccacttt gactggaaca gagttttcag ccactgtgcc gcttgaaatt
     2281 ggagccaaca cgttgacagt gaaagtggtg gatgcggctg gaaatatcaa ggaaaccacc
     2341 caaaccatta ctcgacaaaa cactgaaccg ctccaactga gtgtcactga acccgctgaa
     2401 gggtcattgc tcaatagcaa tccggtgtta tttgctggaa acgtgactgg ttccaatggc
     2461 tcggcgacaa cggtaactgt tttggataaa acgttttcgg tttccccgga gggtacattt
     2521 ggcggatacg ttgatctgag gcaaggcccc aacgtggttc cgattgtagc gacggacgca
     2581 actggaagac gatctgaagt cattcggaat gtgacgctgg atgctttcgc gcctgttttt
     2641 cagaatgtga cgcccgccga tggaagtctg attgccgcca attcagtggt tgtccgtgga
     2701 acgattgagg atgaccagcc ggtcacactc ttcatcaatg gcacacaaac cgacctggtg
     2761 tccggagctt ttgtttcagc cgtgatcccg attcaggaac agattgaaaa cagcattcgg
     2821 ttggtcgcca ttgacgaggc aggaaatgaa gccaccctgt acttaaaatt gatcggcaag
     2881 gatttgacgc ccccagttcc cccaaccatc tttccggttg tttcgccaac ccggcttgac
     2941 actcaaacga ttgccggacg cgccgaacca gggacggcca ttgccattaa cggtgggatg
     3001 tccccggtca gcgttcgggc gtcgagcgga ggtggatttt tcctggccag tattccgctg
     3061 gccgaagggt tcaaccagtt gcaggcggtt gccaccgatg cagcggggaa tgtttcaacc
     3121 acggcttcct tcgcgatgac tcgaaacgca ctgcttcctc cgcctccgcc gggtcaggcg
     3181 tggcaaatca acgtcggtac gggagatgcc caacacgggc tggtcaagat ggaattaccc
     3241 cgcccgctgg tggtgtatgt gtcagatacc gccggaagtc cggtggctgg agcaccggtc
     3301 acgttttcag ttcttcaagg ttccggacag tttgccgatg gtcaacacac cctggccacg
     3361 acgaccaatt cccaggggta cgccaccgcc cgttatgtcg cgggagacct gccgggcacg
     3421 gatgtggtgc gggctgattt tgctgggaat actgccgtgc caacccaatt ctttgcaacg
     3481 atttatcgac cgattccagg tggaacaact cagtttttgg gattggtgct ggatcagaat
     3541 ttacgggctt tgcccaatgt gctggtccgt ctggctggac aacaaacccg aaccgacgac
     3601 aaaggatatt tccagtttca aaacgttcct tcgggtccgc atcagttatt ggagttgatc
     3661 ggtcgcgacc agatccagct tccgggccgc tggccgaata tttcgtatga cgtagacatc
     3721 ttgccgggaa ttgacaaccg gattgaccgc ccgctctttc tgcccaaagt caatgaaggg
     3781 attgaatttc cgctcgatca aaataatgtc gtgaccgagg acacaaccta cgaacttccc
     3841 gtcgtcggag gtcagccccc aatccgggtg acagccagag ccggaactca gatcaacttc
     3901 ccaccggatg tgtcggaccg acgattttcc gtgacccgca ttgccgccaa tcggattcca
     3961 atggcgcttg agaacgggct cacaaccaat ctgtatgtgt cggttcaacc ctcgggtgcg
     4021 atctttgacc ctccgttgga agtcgagttt ccgaatttgg acgggttggc acccaacagc
     4081 ccggtattgt taatgagctt tgaccatgat gccggacgct acgttcaggc tgggaccggg
     4141 acggtggatg aaaccggtca aattgttcgc ggcaaccctg gaagcgggat tcgggttggc
     4201 gcctggcatg cgtttccgcc cacggcacca ccccctgatg gatggttgca ggcggcaaat
     4261 ccgtcaagtg tttcaatcga ggacccagta ccctgttatc caagcccact tcggtatagt
     4321 tgcacgccac cttttgggac gaatctggta acgtgcgatg acgatttgtg ctggatgaga
     4381 ggcgttggag ttggcaactt tgagacagtg tgtcagcaag tgttcacgat caatctcaat
     4441 gatgataatt acatcaccga tgccaatctc cttgcgattc gatatggaac ctggggggcc
     4501 gcgaatgatc aggggcaaat gattggagtg ctgatcgaaa gcattgctaa cccgcgacgc
     4561 tcaatgatca aactataccg cttcaaaagc gatggagctc aacacgatgt gaaaaccatt
     4621 cttctgcctg aattaccacc accaagtgct cgtaccacga aatttgaaat ttcccgagat
     4681 ttccgatttg tgaactttta tgggcctgct aacaataatg accagtttgt agtcttcacg
     4741 actgaagacc aacgtaaggg agacgatttt gaacggcata cgtccaaggt ccgaatctat
     4801 acttcaggtg gagcactggt ccagtgcgtt gacaacactg ttccaactca gtcgtcaacc
     4861 agagatgaga ttgatattgt ctcgtttgtg agtgattgcg acggtaatcc tgattcaaat
     4921 aacggaatca acctcgccga ttgtgatagg acagagaaga ttggaaccag aattggagac
     4981 tttcgaaccg ttatggcgaa taacagggaa tatgcctcca ttatcccatc caacctccga
     5041 attgataaaa agttcgcggc aacccaagtt tatttcatcc gtggattaga ggtattcaat
     5101 cccaacaccc ctatgctcaa tctgaaacga gtcggctttc gcaatgcgga aaatggggga
     5161 gatggcaaac cagtcggtgg tgacacaatt gaacgggcga atgtggcgac tggggattta
     5221 acttttcccg atagcacgac gaaacctgga cttatcgtgc tttcaaagag tttcttgttg
     5281 gtgtttgacc caatagccgg cgagcttgtc cgaacacgtg ttgtgagtga caatgggatt
     5341 cgcggcccca agggacccaa tggagaacca ccaccaaccg aaccaatttc tgcgaatgat
     5401 ttcacccctt tgtttgatat gccagtcgcc tattcgggca atattattgg tcccaaagtg
     5461 gtctcaggta aattcaccgc ccccacggat tcaaatgggg tagagatctt taacacgcca
     5521 cagggccgcc gctacggaat gatgcggttg tttaacaaga cactgtttgt ggcggcgcac
     5581 agcttaccga atgtaatcaa taacacccga ccgtcctgtg acggcaccag gagcgacatc
     5641 gtaggcccat ttaactcgat ctacacgagc tacagcagct tttttgcagg aggactgttc
     5701 ttgcccggcg gttggagcgg caaaccattt ccagaagagt tgggtgtttg tgaaaatcaa
     5761 acgcttg
//
LOCUS       DAOQDO010000069         5751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8641_length_5751_cov_3.313729, whole genome shotgun sequence.
ACCESSION   DAOQDO010000069 DAOQDO010000000
VERSION     DAOQDO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8641_length_5751_cov_3.313729"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKZ53_01940"
     CDS             <1..375
                     /locus_tag="PKZ53_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39221.1"
                     /translation="CRLNYPLGLVIDRDGNLWLASGFNDAIRVIRQVAVGLRTVTVAQ
                     VSFRDRQLTITGTGFGSTGAQVLINETDATAFLKSQTDTSLMLKGKKKKLGLINGTNL
                     IRVIVGGVSSNQFEFEISQDEF"
     gene            complement(563..4774)
                     /locus_tag="PKZ53_01945"
     CDS             complement(563..4774)
                     /locus_tag="PKZ53_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39222.1"
                     /translation="MLAIISGGRLVVCGAEKAATTPSQTNEIYPEGRPTFQLFNDHNG
                     LPQNTVTSIAKDQNGYLWIGTQDGAAVYNGRNWRLVNMPNKSESNYINCILTASDGSH
                     WFGTDAGLIHLTRNNTWAIYQPQNSSLPNLVISCLGETVENGHSTIWVGTLGGGLAQV
                     SFPNPNSPQWNLLNTQHPGFLSNSVTAVWVDTAQAHGTSVWVGCQDGKLVHFVNGHPE
                     SVLLKSSREPEISISSVLGRYRNNQFVLSVGTEQTGVIQLTQDLSQSQTAFQITRWNS
                     HNSDLPGDKVLSLSELQLQNENWLLVGTKDGLCQIGETSVRSFTTSNSDILHNNVRCL
                     LVDTAQPQPLVWIGNDRGLVRYLAGGWTTIDQRNSGIVNTDVYAFLETGPPEKPTLWF
                     GTPDGLSTVCAGQWTTFTTANSKLPNDGVNCFLETVDLNAQSKIWIATEGGLATIAQG
                     RWEVFTTQNSGLPHNRVYALLETIEDGQSTVWVGTRGGGLAKFKNGQWTTYTTQNSGL
                     SNNDIYILTATNVEGKSTLWIGTRLGGLCSLSKNQWTTYTPQNSLLPNGWINCLRQST
                     LNGKPVLWVGTDNGAVCFDLTNPQLPWLVLGEETKPALPNGMILQITEDFQHRLYFYT
                     NKGIAQLTVPPPELTDHQHLMIHTFTTADGLPTNGFNQWGGMTDRRGFIWAGSVEGIS
                     IYNPDFRTRNLLTPPLHLERFTIENYPSPQWDQPRVELSHDQNNLTFEVALLSFYNES
                     QIRYQFFLDGFHRNPTPWVNDNKQVFTNLPPGNYTFSARGMDANGNMSHPILMKFRIE
                     RPPWSSWWAMIGYISLSGGLIYYLVGLRTRALRYYNRKLEFKVRQRTALLEQSQLLLK
                     HNADELAEAVQQLQTSEQQARQAKVESEKANQAKSEFLANMSHDLRTPLNAIIGMTGV
                     LRDTPLNPEQKECVDIIRIGGDSLLSVITDILDFSKIEADMMEFEYLPVVIRECIEDV
                     IDLLAPSALRKHLEFVYWIDPAVPYCVITDAVRLRQVLINLINNAIKFTQTGEIFISV
                     TAQGCEPPHCLLRVAVKDTGIGVPAEKMDRLFQSFSQADTSTTRRFGGTGLGLVICKQ
                     LIEKMGGHLEVESQLDQGSTFSFSLPVTISHELTPDHEVDTTCLKGKQILIVTASQTS
                     RKIWQQLVTHWGMTPVTAGSVEEVGSHLKAHTQFEVGILDHQMPDTDGINLAAQCRTH
                     PAFQNFPLLLFSMAGVEVDRTAYQQNGFVTTLNKPVKPAVLAEALVQVLSSRTPRPPK
                     SVEVQTRPSLSSSQPLKILLAEDNVINQRVILRILEKIGFQAAVATNGYEVLEALRQQ
                     SFDLILMDIQMPEMDGLEATRRIIAEWSSDQRPKIIALTANAIQGDRETYLEAGMDGY
                     LSKPINIDLLQDTIQALLKPVVSSQ"
     gene            complement(5184..>5751)
                     /locus_tag="PKZ53_01950"
     CDS             complement(5184..>5751)
                     /locus_tag="PKZ53_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39223.1"
                     /translation="ILHRWNLLNLNHQKRSFRVADEQVVMILGPNNFIFSGRENEIVV
                     YDHVTGQQLYAIHHPFGGVNHIAFSKDGAQFCVASGGRPDSDLSELSLWETKTGRSIR
                     TFSQKQNPFLLSAVCPNNQYVLSADLIHQPVSGIIRLWETSTGKQLTQVQADSQEVRA
                     CVILNNGTHIVTAGVDGIVRLWTVQIPG"
BASE COUNT         1416 a         1350 c         1470 g         1515 t
ORIGIN      
        1 tgtcgcttaa actatccgct gggactcgtg attgatcgag acggtaacct gtggctggcg
       61 agcggcttca acgatgccat tcgggtcatc cggcaggtgg cggttgggct tcggacggtt
      121 accgtggctc aggtcagttt tcgggaccgg caattgacga tcaccggcac cgggtttggt
      181 tcaaccggcg cccaggttct gatcaacgaa accgatgcca ccgcatttct caaatcccag
      241 actgacacca gtttgatgct caaagggaag aaaaagaaat taggtctcat caatggcact
      301 aacctgatca gggtgattgt gggtggtgtg agttctaatc aatttgagtt tgagatcagt
      361 caggatgagt tttaatgcca gtcagtagtc agtagttagt agtcagtagc tccctcactc
      421 catttttttt ggttgtttag ctactcattc atacaaacga ttcattccaa cacggtattc
      481 gtttggaaac aggttctaaa ttgagtgtga atcatggagt acacttgacg gaaatcctgc
      541 aatcctaaca ttcgactgct ggctactgac tactgactac tggttttaag agtgcctgga
      601 tggtgtcttg caggaggtca atgttgatgg gcttagagag atatccgtcc atcccggctt
      661 caaggtacgt ttcccggtcg ccctggatgg cgtttgcggt gagggcaatg attttggggc
      721 gctggtcaga tgaccattcc gcgataatcc ttcgggttgc ttccagtccg tccatttcag
      781 gcatttgaat gtccatcaaa atcagatcaa atgattgctg ccgcagcgct tcaagaactt
      841 cgtacccgtt ggtggcgacc gcggcctgaa acccgatttt ttccagaatc cgcaggatca
      901 cccgttgatt gatgacatta tcttcagcca gcagaatttt gagtggttga cttgaactga
      961 gactgggtct ggtctgaact tcgactgatt tgggtgggcg aggcgttctg ctactcaaca
     1021 cctgaaccag ggcctccgcc aaaaccgctg gtttgacggg tttgtttaag gttgtgacaa
     1081 agccattttg ttggtaggcc gtacgatcaa cttcaacccc agccattgaa aacagaagca
     1141 gtggaaagtt ctggaaagct ggatgggttc gacactgagc ggcaaggttg attccatcag
     1201 tgtctggcat ctgatggtcg aggattccaa cctcaaactg ggtgtgcgcc ttaaggtgtg
     1261 aacctacttc ctcaaccgag ccagccgtga ccggagtcat tccccagtgg gttacaagtt
     1321 gctgccatat cttgcgactg gtttggctcg ccgtgacgat caggatttgt ttccccttca
     1381 aacaggtggt atccacctca tgatctggcg tgagttcgtg actgatcgtg actggcaagg
     1441 aaaaagagaa ggtcgaaccc tgatcaagct ggctttcaac ctccaggtga cctcccatct
     1501 tttcgatcag ttgtttgcaa atgaccagtc ccaggcccgt tcctccaaat ctgcgggtgg
     1561 ttgacgtatc tgcctgacta aatgactgaa agagccggtc cattttttcg gctggaactc
     1621 caatcccggt atctttgaca gctacccgga gcaaacagtg gggaggctca catccctggg
     1681 cggtgactga aataaagatt tcaccagtct gggtaaactt gatcgcattg ttgatcagat
     1741 tgatcagaac ctgacgcaat cgaaccgcat cagtgatcac acagtaggga actgccggat
     1801 caatccaata cacaaattcc aggtgttttc tcagtgctga cggtgccaga aggtcaataa
     1861 cgtcttcaat acactcgcga atgaccacgg gcaggtactc aaactccatc atgtcggctt
     1921 cgattttgga aaaatccaga atatcggtaa taaccgacag cagggaatct ccgccaatcc
     1981 ggatgatatc aacacattct ttttgctccg ggttgagcgg ggtatcacgc agcacacccg
     2041 tcatgccgat aatggcattg agtggcgtgc ggaggtcgtg gctcatattg gccagaaatt
     2101 cgctttttgc ctgattggct ttctctgatt ctactttggc ctggcgggct tgctgctcag
     2161 aggtttgtaa ttgttgaacg gcttcagcca gttcgtcggc attgtgttta agcaggagct
     2221 gtgattgttc aagcagtgcc gtgcgttgtc gaaccttgaa ctccagttta cgattgtagt
     2281 atctcaaggc cctggtccgc agcccaacta aatagtaaat cagtcccccg ctcagggaaa
     2341 tatacccaat catggcccac cacgaagacc agggaggccg ttcaatccga aacttcatca
     2401 agattggatg tgacatattt ccgttggcat ccatcccacg ggctgaaaag gtgtaattgc
     2461 caggcggtaa attggtaaaa acctgtttat tgtcgttgac ccagggagtt gggttccggt
     2521 gaaatccatc caggaaaaac tgataccgga tttgtgattc gttgtaaaaa ctgagcagtg
     2581 caacttcaaa tgttagattg ttttgatcat ggctcaattc aacccgtggt tgatcccatt
     2641 ggggggacgg gtagttttca atggtgaaac gctccaggtg cagtggtggg gtgagaaggt
     2701 ttcgggttcg aaaatcagga ttgtaaattg aaatgccttc aaccgagcct gcccaaataa
     2761 atccccggcg gtcagtcatt ccaccccatt gattaaaccc attggttggt aagccatcgg
     2821 cagtggtaaa ggtatgaatc atcagatgtt ggtgatcggt gagctcaggt ggaggaacgg
     2881 tgagctgtgc gatgcctttg ttggtgtaaa agtacagccg gtgctggaaa tcctcggtaa
     2941 tctgtaaaat catgccattg gggagagccg gtttggtctc ttcaccgagc accagccatg
     3001 gaagctgcgg gttggtcagg tcaaaacaaa cggcgccatt gtcggttcca acccagagca
     3061 ctggttttcc gttgagggtg ctctggcgca ggcagttgat ccagccgttg gggagcaatg
     3121 agttttgagg cgtgtaggtg gtccactggt ttttgctcaa actgcacaag ccaccaagcc
     3181 tggttccaat ccacagcgtt gattttcctt ctacgttggt ggctgtcagg atataaatgt
     3241 cattgttact caagcctgaa ttttgagtgg tataggtggt ccattgacca tttttgaact
     3301 ttgccagccc gccacctctg gttcccaccc acaccgttga ctgtccgtct tcgatagttt
     3361 ccagaagggc atacacccgg ttatgtggca acccggagtt ttgagtggta aagacttccc
     3421 accgaccctg ggcaatagtc gccagcccgc cttcggtggc aatccaaatt ttggattggg
     3481 cgttgaggtc aaccgtttcg agaaaacaat tcactccatc attgggcaac tttgaatttg
     3541 cggtggtaaa tgtggtccat tggccggcgc aaaccgtaga caggccatcc ggtgttccaa
     3601 accagagggt tggtttttct ggcgggccgg tttcaagaaa ggcatataca tccgtattga
     3661 cgattccgga attgcgttgg tcaatcgtgg tccatccacc ggccagatat ctgaccagac
     3721 cacggtcatt cccaatccag accagtggtt gcggttgtgc cgtatccacc agcaagcagc
     3781 gtacattatt gtggagaata tcagaattgg atgtggtgaa cgatctgacg ctggtttccc
     3841 caatctggca caacccatct ttcgttccaa ccagcaacca gttctcattc tgtaactgca
     3901 gttcactcag agacagaact ttatcgccag gcagatcgga attatgcgaa ttccaccggg
     3961 ttatttggaa cgctgtttgg gactgagaca gatcctgggt gagttggatg actccggttt
     4021 gttcagttcc aaccgagagc acaaactgat tgttccggta cctgccaagt acggaactga
     4081 ttgagatttc tggttcccgt gaacttttga gtaatactga ttctggatga ccattgacaa
     4141 aatggaccag ttttccatcc tggcagccta cccagacgct ggttccgtga gcctgagctg
     4201 tgtcaaccca cactgccgtc acggaattac tcaaaaaacc tggatgttgc gtgttcagga
     4261 gattccactg gggtgaattt ggattgggaa agctgacctg ggccagtcct cccccaaggg
     4321 tgccgaccca aattgttgaa tgaccatttt cgactgtttc tcccagacaa ctgatgacca
     4381 ggtttggcag acttgaattc tgaggctgat agattgccca ggtgttattt cgggtgaggt
     4441 ggatcagtcc ggcatcagtt ccaaaccagt gactcccatc gctggctgtg agaatacaat
     4501 tgatgtaatt ggattccgat ttgttgggca tattgaccag ccgccagttt ctgccgttgt
     4561 aaactgcggc tccgtcctgg gtgccaatcc acaaatatcc attctggtct tttgcaattg
     4621 aggtaaccgt gttttgaggc agcccattat ggtcattaaa cagttgaaag gttgggcgcc
     4681 cctctggata gatttcattg gtttgagagg gagtcgtcgc tgctttttcg gcaccacaca
     4741 ccaccaaccg cccaccactg ataatggcca gcaccaaaag tactatcctc aggaaaaacc
     4801 aatgctgcat tgataatttt gccagacctg gagagtatgg aatcaaaaga aaccggaagg
     4861 ggctcaacaa ttgcaggagt gaagtgaagg gtaacttgag gataccttat tctgtgggtt
     4921 gaagaaagtg gactgacgaa gattgggcaa tcttcagttc caggttttga gccgttcagg
     4981 taagtacctt gccgaaaatt tccagacatt ttgaaccacg aaatacacga aaaaaccaaa
     5041 cactgccaaa atgtaagatc tcaggaacct tttgacaagg tacttatcga ggtgggttcg
     5101 gcactccgga aggaatgcca agaagtacag agttcaacct gtaggtgact catttcaaac
     5161 aactttcgat caaagatcaa agatcaccca ggaatttgca cagtccaaag tctaacgatg
     5221 ccatcaactc cagccgtaac aatgtgtgtt ccattgttta aaattacaca tgcacgaact
     5281 tcctgagaat ctgcctgaac ttgagtgagt tgtttgccag ttgaagtctc ccaaagtcgg
     5341 atgattccgc tgactggctg gtggatcaaa tcagccgaga gcacatattg attattggga
     5401 cagaccgctg acagaagaaa tggattttgt ttttgggaga atgtgcggat tgatcgtcct
     5461 gtttttgttt cccaaagtga gagttcactc aaatcagaat caggccgacc tccgcttgcc
     5521 acacaaaatt gggcaccatc tttggaaaaa gcgatgtgat tgacaccacc gaacggatgg
     5581 tgaatagcat aaagctgctg accagttacg tggtcataaa ccacgatttc attttccctg
     5641 cccgaaaaaa tgaagttatt tggacccaaa atcatcacta cctgctcatc cgcgacacga
     5701 aaagatcgtt tttggtggtt caggttgagc agattccagc gatggagtat c
//
LOCUS       DAOQDO010000070         5744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8658_length_5744_cov_3.547196, whole genome shotgun sequence.
ACCESSION   DAOQDO010000070 DAOQDO010000000
VERSION     DAOQDO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8658_length_5744_cov_3.547196"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKZ53_01955"
     CDS             complement(<1..255)
                     /locus_tag="PKZ53_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39224.1"
                     /translation="MKTPNRKPFIPEWTHGVDFVSRKKYLMGLNDLFSTYCEHCSHAN
                     NLLVLVFIQKCSVLLLPKDTVTSWIKREVTEDGKMGITGRL"
     gene            602..4735
                     /locus_tag="PKZ53_01960"
     CDS             602..4735
                     /locus_tag="PKZ53_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39225.1"
                     /translation="MAKTVFHRLLGFVCLLAGLAPGIAGVSLLVGKPLGAAPPPLVSQ
                     RASQSSATPLSTAKDITTTGRYGFRIFTDRDGLPQNSITSITFDLKGYLWVGTLDGIA
                     CYNGRKWIVKKLPNRQASNYVRTLVAMSDGSLWVGTSGGLSILKNDQWSTFDEKSGLP
                     NNEVRAILESKAVDGSPEYWLGTDGGLVCLRNGAPVVPISADELPDQSIRCLMETRDP
                     IGNRIIWAGTRSGLVRIENNRVSVLTTKQGLPSDFVFSLVETGAGTHHQALWVGTQRG
                     LAKLENGSWTIYTRASGLPDDTVFCLLASESVTGSPVIWAGCAGRGLGRLENGTWTTI
                     SQREGIPYNVVWSLRETQSETGARTLWVGTAGGGLARLETGKWVALTTLTGLPSNSVY
                     SIFESISPVGESSFWFGTTLGVARFEKGEWVPVPGPLEQAAVNVFYETTDAEGTRTLW
                     AGFDRGLARYHNNQWETFHSDATSGQYHITSILQVTLPAQPSELWLGTKTGLVKFGQN
                     QLVPIRDLSTPLLEHHVRCLAQTRLASGKTVVWVGTRQGLACLRDGVWHTYDVTNGLP
                     HNDVLSLQELETRDGRHELWIGTFGGGVACLDLTSDEPHWTYYSDSTTPALPNNVIYQ
                     IRADFKDRLYFFTNKGVVRLTQGKIPALSPEAQRVEIFTTEDGLPSNECNTGASWVDS
                     RGRIWAGTIDGAAVYSPANEIETRAPCPLIIERAWLTGSDQPVVNHSVLAHSDNNLWF
                     EYTLLSYYRENDTQYQTQLIGLDEKPSAWTNDYKKEYTNLPAGDYTFRVWGKDFAGNV
                     SGPVSFSFRIKPAPWVTWWAVALYICLAIGTGYLVILWRVQALQRQNQLLEEKVSQRT
                     QVIAEQVDKLTISEQLALAAKEEALQAKNQAVAANQAKSTFLATMSHELRTPLNAILG
                     FVQLMQRDQHLSTDQHENLDIIMRSGEHLLALINDVLSISKIESGRVTLNQQNFNLQK
                     MLQGLEEMFRPRAESKNLRFCFNLDPQLPHHVLGDESKLRQILINLLGNAFKFTDYGS
                     VELIVNWKKDRVEFQVCDTGQGISDEDLPEIFKAFVQSETGRRMKEGTGLGLAICQTF
                     VQLMGGEIKAESTHGHGSRFSFEIPLMGVEFEEKSQIERKVIGVEPGQPSFRILIVDD
                     KWENRRLLVKMMNSIGFEVKEAVNGKEAVDLWMQWNPHLIWMDMRMPIMDGYTAIRKI
                     RGYEIGIRMREYEISRSDGGRIRKEDTLRQNSGRGRGIRTVIIALTASAFDESRNTIL
                     AQGCDDYISKPFRDSVIFEKMTQYLGVKFVYRDLAATTAEHRINTDALQAFLAEQSPE
                     VLATLNKAANEGDLEATYEVIDQIDQGQHHDLADKLRAMVKAYQFDELLDLIEGVAK"
     gene            4997..5506
                     /locus_tag="PKZ53_01965"
     CDS             4997..5506
                     /locus_tag="PKZ53_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HNJ39226.1"
                     /translation="MPLATSGHKGVSRIDHPAKQMHGWYVRVPFRGQIHSKFFSDSKH
                     GGKEKALKKAVQWRNKIEREIGKPRTDRPVVTASPRNNTGVIGVQRMVQIRQNKHAPP
                     KEYPIYVVSWQPEPGVLKREIVPINKYGEEKAFMKACAIRRKREREMFGKSIVPTATD
                     LTLTEGDAS"
BASE COUNT         1431 a         1520 c         1449 g         1344 t
ORIGIN      
        1 taatctgcca gtaatcccca tcttgccatc ttctgtcact tcccttttta tccaggatgt
       61 cactgtatcc tttggcaaca acaacaccga acatttttgg ataaatacta aaaccagcaa
      121 attgttcgca tgcgaacaat gctcgcaata agttgaaaat aaatcattta accccattaa
      181 gtattttttc cgagacacga aatcaacacc gtgggtccat tctggaatga acggcttgcg
      241 gtttggagtt ttcaaatcca ggtcattgta tccctggctt ccctgtcatt ttgtcagcca
      301 ggtgtcaggc aaattcccga ccaggcgact gagaagttgc tcaactccga agtcagcatc
      361 aatcaaagca aaccgttgat tctattgaac aaaacagcct gaaccctgaa ccctgaaccc
      421 tgaaccccga gccctaattt gccattcctg ttttccactt cttcaatttc agctagtatt
      481 gggcggctcc gacatcctgc ccaggtcaat gattccccaa attgcgagga tcttttttcg
      541 actggtgttc acgctttttt aaagccagaa ccccatcgaa gaaatgaggc ttgctctggt
      601 tatggcaaag actgttttcc accgattgct tgggttcgtc tgtttgctgg caggtcttgc
      661 gccaggaatt gcaggcgtgt ctcttctggt cggaaaaccg ctgggtgccg ccccaccacc
      721 gctggtttct caacgagcaa gtcaatccag cgccactcct ctttcaaccg ccaaagacat
      781 caccaccacc gggcgctatg gatttcgaat ctttaccgac cgcgatgggc tcccacaaaa
      841 ctcaattacc tcaatcacct ttgatctcaa ggggtatttg tgggttggca ctctggacgg
      901 cattgcctgc tataacgggc gcaaatggat cgtgaaaaag ctacccaacc ggcaggcgtc
      961 caactatgtg cgaaccctcg ttgcaatgtc cgatggaagt ctctgggttg ggacaagcgg
     1021 tggcctgtca attctgaaaa atgatcagtg gagcaccttt gatgaaaaat ccggacttcc
     1081 caacaatgaa gtccgggcga ttttggaatc aaaagcggtt gacggttcgc cggaatactg
     1141 gctgggcacg gatggtggcc ttgtctgtct gcgcaatggt gccccggttg ttcccatatc
     1201 ggctgatgag ttgcccgatc aatccattcg gtgtctgatg gaaaccagag acccgattgg
     1261 aaaccggatt atttgggctg ggacgcgcag cggactggta cgaattgaaa ataaccgggt
     1321 ttccgtgctg acaactaaac aggggttgcc gtcagatttt gtgttttctc tggttgaaac
     1381 cggcgccggc acccatcacc aggccctgtg ggttggcacg caacggggat tggccaaact
     1441 tgaaaatggt agctggacaa tttacacccg tgcctccggt ttaccggatg acacggtttt
     1501 ttgtctcctg gcctcagaat ccgtcaccgg atcgcccgtc atctgggcgg gctgtgcagg
     1561 tcgcggcctt ggccggcttg aaaatggcac ctggaccaca attagccagc gtgaaggaat
     1621 cccctacaac gtggtctgga gcctgcgcga aacgcaatcc gaaactggcg cccgcaccct
     1681 ctgggttggc acggctggtg gcggactggc acgactggaa accggaaaat gggtagcgct
     1741 gaccacattg actgggcttc ccagcaacag tgtctatagc attttcgaat ctatttcccc
     1801 agttggggaa tcaagtttct ggtttggtac cacgctgggg gtcgccagat ttgaaaaagg
     1861 cgagtgggtt ccggttcccg gtcccttaga acaggccgcg gtcaatgtgt tttatgaaac
     1921 aaccgatgct gagggcaccc gcacactctg ggccgggttt gatcgagggc ttgcccgcta
     1981 tcacaacaac cagtgggaga cctttcactc tgacgcaacc agtggccaat accatatcac
     2041 cagcatcttg caggtaacgc tccccgctca gccatctgaa ttgtggcttg gcaccaaaac
     2101 tggcctggtc aaatttggcc agaatcaact cgttccgatt cgcgatctca gcacccccct
     2161 tctggaacat cacgtgcgat gtctggccca aacccggctt gcaagcggaa aaacagtggt
     2221 ctgggtcggg actcgacaag gactggcctg cctccgcgat ggcgtctggc acacctatga
     2281 cgtcaccaac ggtctgccac acaatgacgt tctctcgctg caggagcttg aaaccagaga
     2341 tggtcgccac gaactctgga ttggcacctt tggcggagga gttgcctgcc tcgatctgac
     2401 cagtgacgag ccacactgga cctactattc tgattcaacc acgccggcgc tccccaataa
     2461 tgtcatttat caaatccggg ctgatttcaa agatcgcctg tatttcttta ccaacaaagg
     2521 cgtggtgcga ctcactcagg gcaagattcc ggccctgtct cctgaagccc agcgagttga
     2581 gatctttacc accgaagatg gcttgccgag caatgaatgc aacaccgggg cgtcatgggt
     2641 tgatagccgg ggccgcatct gggccggcac cattgacggg gcggcggttt acagtccagc
     2701 caatgaaatt gaaacccggg ccccctgccc actcatcatt gaacgggcct ggctcaccgg
     2761 gtcagaccag ccagtggtca accactcagt tctcgcccac tcagataaca acctctggtt
     2821 tgaatatacc ctgctgagtt attaccgcga gaatgacacc cagtaccaga cacaactgat
     2881 tggcctggat gaaaaaccct cggcctggac caatgactac aaaaaggagt acaccaattt
     2941 accggctggg gattacacct ttcgcgtctg gggcaaagat tttgccggca atgtctcagg
     3001 accggtctcg ttttcatttc ggattaaacc ggccccctgg gttacctggt gggcagtcgc
     3061 cctctatatt tgcctggcca tcggcactgg atatctggtc attctgtggc gggttcaggc
     3121 cctgcaacgc caaaaccagt tgcttgaaga aaaagtgagc cagcgcaccc aggtgattgc
     3181 cgaacaggtt gataaattga ccatttccga acaactggca ctggcggcca aagaagaagc
     3241 cttgcaagcc aaaaaccagg ccgtcgcggc caatcaggcc aaaagcacat ttctggcgac
     3301 gatgagccac gagttacgca cgccgctcaa tgcgattctc ggttttgtgc aattgatgca
     3361 acgcgaccag cacctgtcaa ccgaccagca tgagaatctc gacatcatta tgcggagcgg
     3421 cgagcacctg ctggcactca tcaacgatgt cctgtcaatt tcaaagattg aatctggccg
     3481 cgtcaccctc aatcaacaaa atttcaacct ccagaaaatg ctgcagggcc tggaagagat
     3541 gtttcggcca cgagcagagt ccaagaacct gcgcttttgc tttaacctgg atcctcaact
     3601 tccccaccac gtgctcggcg acgagagcaa actgcggcag atcttaatca atctgcttgg
     3661 aaacgccttt aaatttaccg actatggatc ggtggaattg atcgtcaact ggaaaaaaga
     3721 tcgagtcgaa tttcaagttt gcgataccgg tcagggaatt tctgatgaag acctgcccga
     3781 aatttttaag gcatttgtgc agtcggaaac tggacgccgg atgaaggaag gcaccgggct
     3841 gggactggcc atctgtcaaa cctttgtcca gttgatggga ggtgaaatca aagcggagag
     3901 cacacatggg cacggctcgc ggttctcgtt tgaaatcccc ttgatggggg ttgaatttga
     3961 ggaaaaatcg cagatcgaac gcaaagtcat tggcgtcgag ccaggtcagc ccagttttcg
     4021 gattctgatt gttgatgaca aatgggaaaa ccggcggttg ctggtcaaaa tgatgaattc
     4081 aatcggcttt gaagtcaaag aagccgtcaa tggcaaagaa gccgttgatt tatggatgca
     4141 gtggaatccg cacctgatct ggatggatat gcgcatgccg attatggatg gctacacggc
     4201 aattcgcaaa atccgtggct atgaaatcgg aatccgcatg cgggaatatg agatttcccg
     4261 ttctgacggt ggtcggatcc gcaaagaaga cacgctgcgg caaaactccg ggcgcgggcg
     4321 cggcatccgg acagtgatca ttgccctgac agccagcgcc tttgatgaaa gccgcaatac
     4381 cattctggcc cagggctgtg acgattacat ttccaagccg tttcgggatt cggtcatttt
     4441 tgaaaagatg acccagtatc tgggtgtcaa atttgtctat cgcgacctgg cggccaccac
     4501 cgccgaacac cgtatcaaca ccgatgccct ccaggcattt ctggccgaac aatcccctga
     4561 agttctcgct accttaaaca aagccgccaa cgaaggcgat ctcgaagcca cctatgaagt
     4621 cattgatcag attgatcagg gacagcacca cgacctggct gataagctcc gggccatggt
     4681 caaagcctat cagtttgatg aattgctgga tttgattgaa ggcgtggcga agtagcgaac
     4741 cgacagttga ggagcaagtc ccgaggcgtt acgcttcaga tgggtgtctg tcaacccatc
     4801 attcttgtga ttttgactcc tttgcgcctt taccaggttg cgccttcact ccattttgct
     4861 atttcgcttt tcgaataaag aaattctcac aatcttgttt catattcccc aacatcggca
     4921 tataatcgag actcctgccc ctttgtgatc ccaaataatt ctgatgttac attttacttt
     4981 gaaggagtcc cttacaatgc cgctcgctac cagtggtcat aagggagtga gtcgaattga
     5041 ccatccggcg aagcaaatgc acggatggta tgtccgcgtc cccttccgcg gccagattca
     5101 ttccaaattt tttagtgaca gcaagcatgg cggaaaagaa aaagcactca agaaggcagt
     5161 tcaatggcgt aataaaattg agcgagaaat tgggaaacct cggacggatc ggcccgtagt
     5221 cacggccagt ccgcggaaca acaccggtgt catcggtgtt caacgaatgg tgcaaatccg
     5281 gcaaaacaaa cacgcaccac ccaaggaata cccaatttat gttgtttcct ggcagcccga
     5341 gccaggcgtg ctcaaacgag agattgtccc gattaacaaa tacggcgagg aaaaagcgtt
     5401 tatgaaagcg tgtgcgattc gccgtaagcg tgaacgagaa atgttcggga aatcaattgt
     5461 accaaccgct actgatctca cgctgacaga aggtgacgct tcttaatgcc atgatttggt
     5521 gacattgagg ttcacaacga cggatcctgc ggcccggctc cctttatcaa aacctgctgt
     5581 ttcattgcga aacagcaggt ttttactttt gtcagaaagg taataccaga atattcaaac
     5641 tttcgaatgt aatgacattc agaactaaaa ccatcgggct gaagacattg ggctgaagac
     5701 gtcgggctga agacattggg ctgaagacat cgggctgaag acgt
//
LOCUS       DAOQDO010000071         5642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8895_length_5642_cov_19.022731, whole genome shotgun sequence.
ACCESSION   DAOQDO010000071 DAOQDO010000000
VERSION     DAOQDO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8895_length_5642_cov_19.022731"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKZ53_01970"
     CDS             complement(<1..604)
                     /locus_tag="PKZ53_01970"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNJ39227.1"
                     /translation="MIQEIEKLLGDEASQLLEYKATAVPKETLHLPGPDFIERVWIQT
                     DRNPQVLRSLQQIFSTGRLAGTGYVSILPVDQGIEHSAAASFAPNPIYFDGGNIVKLA
                     IEGGCNAVASTFGVLGSVSRQYAHRIPFIVKLNHNEMLTYPNRFDQVMYGQVEQAWDM
                     GAVAVGATIYFGSSESTRQIQEVSEAFYRAHELGMATILWC"
     gene            1074..1859
                     /locus_tag="PKZ53_01975"
     CDS             1074..1859
                     /locus_tag="PKZ53_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39228.1"
                     /translation="MTQLSVCIVGLDEVEHEEIRSGAFVRLIGHELLPKIQVVDGQLL
                     VESKRGPWFVPVDKVLFHGIFEDDLEFIAALALWGGPCLPNARAMMDCRLRIPCLVRA
                     LEFTRFGAPKRSYSGPKAGFTSEIERVAKWGNWHCGENKERFTGEWKSDEACIIEPFL
                     TGDAVRVVFIGDQYWQIKLEGDTWLKSIHAPNAAFMEPDPELVEDTDRVRKGFGLEIV
                     ANDYIVTPEGNRHLLEVNHIPNVTRFPELWEAYRDFAVRWINK"
     gene            2043..2942
                     /locus_tag="PKZ53_01980"
     CDS             2043..2942
                     /locus_tag="PKZ53_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39229.1"
                     /translation="MKITTCCLWLMIISLAFTFPVSAQTVTWDDLMQSGNQALTQNRP
                     AEAEQSFRDALKLIEKSGDKDPRKPVTLIKLAAACDLQSKRDEAETLANQSLLALEKV
                     PLLSKTQGTTDALAGVNVTLQLYDQAAGLFVSHQKYAEAETTYQKAITYCDKTLSLAS
                     NSKSNDELVKSLALLTTDVEQGQANAHDKLGSVYFLQKKYDLAEVAFHKSLKLREKLF
                     QPTSSPIAESLANLATLFAVQAKYDKAEPLYKQALDIFEQTNSMNQPKALYILGNYVL
                     LLKKTGREAEAKALLEKTKAQSK"
     gene            complement(3201..>5642)
                     /locus_tag="PKZ53_01985"
     CDS             complement(3201..>5642)
                     /locus_tag="PKZ53_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNJ39230.1"
                     /translation="HHQPQVYQVRAGSREVIPGRYQVNQSPTRDRWQITFELGLYNKD
                     LPLVIDPVVKYATLLGGDGATSARAVAVDQAGNCYVAGTTSALFFPHMTGSPQNRGGF
                     DAFVTKLNPSGSAAIYTTFFGGGFDEEAEAIAVDETGSVVVVGTTRSPDFPLERPIQS
                     KFGGFPDAVSSDAFITRLALSGNALEMSSFLGGSAQDAGYGVAIDRQHQVYLTGVTQS
                     ADFPVVRAVQPAFSNLTDAFVAKINAAGTQLVYATYLGGVDFDAGYAIAADAVGNAYL
                     TGSTASNAQTFPTVPAFTIPFGGVTDAFVAKINPAGSKLEFCRYFGGSEFDSGQGIAV
                     DAGGNVYVAGETRSPRLPVVNPIQGALLGATDGFVVKFDSTGLNTTFLTYLGGRNTDQ
                     VQALAVDTSGTVFVAGETQSGDFPLVEPTQINPDDLNLNGFVTRIDPVQGRLMFSTFI
                     GGTASDEVTALAVDSRANIFLAGNTVSANFPLVDPLQPEFCGDRSAFIARIDAVLAPP
                     EAPAGLTAFGISATEIDLEWTDQSTTETGFVIERQFEDPPQFVELITLSANQTRFRDT
                     RLQPATEYRYRVKAVNAGGNSGYTNEAQAKTLAIDDQEPPVVLVLSPNGGEQFARQAP
                     ITLSWATADNRTVVEQAVDLSTDGGQTFELVVATGLAGEITEFLFFPPAEVSTTTGRI
                     RVTALDGAGNQGQDASDADFTINPADETPPQVQIVFPNGGEVLKGGQTVTFQWLSSDN
                     RQVTRQDVRLSTDGGATFSVEVGSGLSGDVQRFDFEIPRRTLKTKRARLQVLVTDEAG
                     NQATDVSDVDFKIKK"
BASE COUNT         1477 a         1456 c         1379 g         1330 t
ORIGIN      
        1 aacaccacaa aatggtggcc ataccgagtt catgtgcccg atagaacgct tcggaaactt
       61 cctgaatctg gcgggttgat tctgatgaac caaagtagat cgtggcacca accgcaacag
      121 cgcccatatc ccacgcctgt tcaacctggc catacatcac ctggtcaaag cggtttgggt
      181 acgtgagcat ttcattgtgg ttcaacttca caataaatgg aatccggtgc gcatactggc
      241 gagacaccga tcccaacaca ccaaaagttg aagccacggc gttgcagccg ccttcaatcg
      301 ccagtttgac gatgttgccg ccgtcaaaat aaatcgggtt tggtgcaaat gaagcggcgg
      361 cactgtgttc aattccctgg tctactggca ggattgacac atatccggtg ccagccagtc
      421 gtccagttga aaaaatttgc tgcaggcttc gcaaaacctg cgggttgcga tcagtttgga
      481 tccaaacccg ttcgataaaa tctggtccgg gcaagtgcag tgtttctttt ggaaccgcgg
      541 tcgctttata ctcaagcaac tgtgaggctt cgtcgcccag taacttctcg atttcctgaa
      601 tcattggaat accctctcta gcaactatgt tgaaattctc cccaggaccg ggtgctggtc
      661 ccagcctcag tatgtgaggt taaggttgtg gatggttctg atgaaagagg cccgcgcatc
      721 atataaggga aacggctgtc ggtaaagcat cttgtgcttt gagactgagc caggaggtgg
      781 ccaattacca gacgagtttc atccaaaata tatgggttca aatacaagac acccagcggc
      841 gtaaggagtt gtaaatcttt tggttatggc ctgacaaagg caaattgcca gtgtagcctg
      901 tggtttgcga tagccccata atccacacgt ttttgttttt catccaataa atatctttca
      961 gctctgactc tggttgactt gcaaaatcag gtaacaccga ttatcaagct taacccacac
     1021 tttctgaata agttggtggg aatcacgtat caagaataag tgagttaaat aatatgacac
     1081 aactgagcgt gtgtattgtc ggcctcgatg aagtcgagca cgaagaaatc agatcaggtg
     1141 cgtttgtgcg gttgatcggc cacgaattgc tgccaaaaat tcaagtggtg gatggccagc
     1201 tcctggttga atcaaagcgt ggtccgtggt ttgtaccagt ggacaaggta ctcttccacg
     1261 ggatttttga agacgacctg gagtttattg ccgctctggc cctctggggc ggcccctgcc
     1321 tgcccaatgc ccgggccatg atggattgtc ggctgcgcat cccctgcctg gtacgggcac
     1381 tggaatttac gcgctttggt gcaccgaaac gatcctattc cggtccaaag gctgggttta
     1441 cctctgaaat tgaacgggtg gccaaatggg gaaactggca ttgcggtgaa aacaaagagc
     1501 gatttaccgg cgaatggaaa agcgatgagg cgtgcattat cgaaccattt ctcactggcg
     1561 atgccgtccg tgttgttttt attggcgacc agtactggca aatcaaactg gaaggtgaca
     1621 cctggctcaa gtccattcat gctccaaatg cagcttttat ggaaccagac cccgaactgg
     1681 tggaagacac cgaccgggtc cgtaaaggat tcggcctcga aatcgtggcc aatgattata
     1741 ttgtcacccc ggaaggcaac cgccatctac tcgaagtcaa tcacattccg aatgtcactc
     1801 gctttcccga actttgggaa gcctatcgcg attttgccgt tcggtggatc aataaataaa
     1861 tccgggttcc gggttctgaa aaaaacaaaa aggacttaaa ggaccaaaag gaccaaaagg
     1921 acataaattc gaaaacccgg aacccggaac ccggaacccg gaacccggaa cccggaaccc
     1981 ggaacccgga acccggaacc cggaacccgg aacccggaac cctattcttt taagtgagat
     2041 ttatgaaaat tacgacctgc tgcttgtggc tgatgatcat cagtttagcg ttcacgtttc
     2101 cggtttcagc acagactgtc acctgggatg atctgatgca atctggcaat caagccctga
     2161 cccaaaatcg cccggctgaa gccgaacaat cgtttcgcga tgcgctcaag ttgatcgaaa
     2221 aatccggcga taaagatccg cgaaaacctg tgacgttgat caaactggct gccgcttgtg
     2281 accttcaatc caaacgagac gaggctgaaa ctcttgctaa tcagtcactt ctggctcttg
     2341 aaaaagtccc actgctgtca aaaacgcaag gcacgactga tgctttggcc ggagtcaatg
     2401 tcacgttgca gctttatgat caggcagccg ggttgtttgt gtcacatcag aaatatgccg
     2461 aagccgaaac gacataccag aaagccatca cctattgtga caaaacgctt tcacttgcct
     2521 ccaattccaa aagtaatgac gaactggtga aaagtctggc tttgctgaca actgatgtcg
     2581 aacaaggtca ggccaacgcc cacgataaac ttgggagcgt ctattttctc cagaaaaagt
     2641 atgatctggc agaagtggcc tttcacaaat cactcaaact ccgggaaaaa ctctttcaac
     2701 ctacctcatc gccaattgct gaaagtctgg ccaatctggc aacacttttt gcggtccagg
     2761 caaagtatga caaggcggaa ccactgtata aacaggcact tgacatcttt gaacagacca
     2821 actcgatgaa ccagcccaaa gctttgtaca ttcttggaaa ctatgtgttg ttgctgaaaa
     2881 aaaccgggcg tgaggctgaa gccaaagctt tgcttgaaaa gacaaaagct caaagcaagt
     2941 gaagagcgca ggcaccatcc gcttgctttt tacgtcgctc ttgcaggacc tgggtctcgt
     3001 cctgaccggg accgtaggct tcgcaccacg gctattgaat gccgcccctg cgggcctcag
     3061 atccttcgct attttgctct gactgccatc tcacttatac caatttgcaa tgaacctaat
     3121 catattaggg gatggtcaaa tagttcaatc aaataaactt agcgagctac tgactactga
     3181 ctactgacta caaactggta ttatttctta atcttaaaat ccacatcact cacatcagtt
     3241 gcctgattcc cggcttcgtc ggtgaccagc acctgcaatc tggcgcgttt ggttttgagc
     3301 gttcggcgag gaatttcaaa atcaaagcgt tgcacatccc ctgaaagccc tgaaccaact
     3361 tcgactgaaa acgtcgcgcc gccatcggtt gaaagtcgca catcctggcg ggtcacctgt
     3421 cggttgtcgg atgaaagcca ttggaaggta acagtttgac cgcctttaag aacttctccg
     3481 ccgttgggaa aaacaatctg aacctgtggt ggcgtttcat cggcgggatt gatggtaaaa
     3541 tcagcatccg aagcatcctg cccttgattg ccggcaccat ccagcgccgt cacccggatg
     3601 cgaccggttg tggtggacac ttcggcggga ggaaaaaaca agaattccgt gatttccccg
     3661 gcaagcccag ttgccacaac cagttcaaag gtttgccctc catcggttga aagatcaact
     3721 gcctgttcaa ccacggttcg gttatctgcc gttgcccagg aaagagtgat tggcgcctgg
     3781 cgggcaaatt gttcgcctcc gttcggagac aaaaccaaaa ccactggcgg ctcctgatca
     3841 tcaatggcaa gcgttttggc ttgcgcttca ttggtatagc ctgaatttcc accagcgttg
     3901 accgctttga cccggtagcg atactcagtt gccggctgta accgggtgtc ccgaaatcgg
     3961 gtttgattgg cagaaagtgt tatcaattca acaaattgtg gcgggtcttc aaattgtcgt
     4021 tcgatgacaa aacctgtttc cgtggtactt tggtcagtcc attccagatc aatttcagtg
     4081 gcggaaattc caaatgccgt cagcccggct ggtgcttcgg gtggggcgag tacagcatca
     4141 attcgggcaa taaaagcact ccggtcacca caaaattcag gctggagcgg atcaacgagc
     4201 gggaaattag ctgacactgt atttccagcc agaaaaatat tcgctctgga atcaaccgca
     4261 agagcagtga cttcatcact ggcagttccg ccaatgaagg tagaaaacat caatcgtcct
     4321 tgaaccgggt caattcgggt gacaaatcca ttcaaattca gatcatccgg gttgatttgg
     4381 gttggttcga ccagcgggaa gtcacccgac tgggtttctc cagcgacaaa caccgttccc
     4441 gatgtatcaa cggccagtgc ctgaacctga tctgtgtttc gtcctcccag ataggtgaga
     4501 aaggttgtgt tgagaccagt tgagtcaaat ttgaccacaa acccgtcggt cgctccgagt
     4561 aaagcaccct gaataggatt tacaactggc aaccggggtg atcgagtttc accagccaca
     4621 tagacatttc cgcccgcatc cacggcaatt ccctgcccgc tatcaaactc gcttccacca
     4681 aaataccggc aaaattccag ttttgaaccg gctggattga ttttcgccac aaaggcatcg
     4741 gtgacaccac caaatggaat cgtgaaagcc ggcactgtgg gaaaggtttg ggcatttgaa
     4801 gcggtacttc cggtgaggta ggcgtttcca accgcatcag cggcaatggc atatccagcg
     4861 tcaaaatcaa ctccgccaag ataagtggca tagaccagtt gcgttccagc cgcgttgatt
     4921 ttagcgacaa acgcatccgt taagttactg aaggctggct gaacggcacg aacgaccgga
     4981 aaatcggctg attgcgtcac cccggtgaga tagacctgat gctgacggtc aatcgccact
     5041 ccgtatccgg catcttgagc actgccacct agaaaactgg acatttccag agcatttcca
     5101 gatagagcca atcgcgtgat aaaggcatct gaggaaactg catctggaaa cccgccaaat
     5161 tttgactgga ttggtcgttc cagcgggaaa tccggcgagc gtgttgtccc aaccacaacc
     5221 actgagcctg tttcatccac cgcaattgct tcagcttcct cgtcaaatcc gccaccgaaa
     5281 aaagtggtat agatcgcagc cgaccctgat ggattcagtt tggtgacaaa agcatcaaaa
     5341 ccgcctcggt tttgcgggga accggtcata tgtggaaaaa acaaggcact ggtggttccg
     5401 gcaacatagc agttcccggc ctgatcaaca gcaaccgcac gagccgaagt ggctccgtcg
     5461 cctccaagga gcgtggcata tttcaccacc ggatcaatca ccaaaggcaa gtctttgtta
     5521 tacagcccaa gctcaaaagt gatttgccat ctatctcttg tcggtgattg attgacttga
     5581 tacctgccgg gaatgacttc ccgagaacca gcccggacct gatatacctg cggctggtgg
     5641 tg
//
LOCUS       DAOQDO010000072         5617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8966_length_5617_cov_3.523912, whole genome shotgun sequence.
ACCESSION   DAOQDO010000072 DAOQDO010000000
VERSION     DAOQDO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8966_length_5617_cov_3.523912"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKZ53_01990"
     CDS             <1..654
                     /locus_tag="PKZ53_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNJ39231.1"
                     /translation="AFLPPATRTLAGGLFIVDRSIDLVTILMLALAGSAGVLTVGWQI
                     ILGTTLLFLVGGVLLWPILLPWMLGWLPLPVKLRRILSELVTGLQALRLRDLGWNLCL
                     GCALMVLDVISLYVLVATFEPVSFKAVTFAFPLILLTNLVPITPGGIGVRESASVFLF
                     RQFGVSQAAAIYSALFLYILNSLLPGLWGITYVRKLGAHPSAVDSSPPEKISEVTLS"
     gene            651..1358
                     /locus_tag="PKZ53_01995"
     CDS             651..1358
                     /locus_tag="PKZ53_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ39232.1"
                     /translation="MNLMGKTVLITGGARRLGRAIALDLATHGANIALHFRSFPEEAF
                     ATLDQLHKCGVQAAAFQANLLQSEELTDMIAQVYRTFGQIDVLINNAAVFDRKSFFEL
                     TETDWDHTLDTNLKIPFLCSRLVGEKMLGQGTGKIINLACVGGVRPWANFIPYSVSKA
                     GLIMLTEALALALAPLIQVNAIAPGRIEFPDHAVPDVPSFPTGQPQDITRAVRYLIES
                     DYVTGETLLVDGGRHLR"
     gene            1503..3746
                     /locus_tag="PKZ53_02000"
     CDS             1503..3746
                     /locus_tag="PKZ53_02000"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39233.1"
                     /translation="MKRTYFTTVLCLLLTLGMAWSPVLFSASAMGSDPDSNSTTANID
                     FEWELNGPFGGTVRSLVQNDANPQQFFLGTSDGQLYVSNDGTQNWTWIPTFNKSGNLI
                     DNLVIDPRDPDTVYLGMWALGEKRDGAIYKSTDSGKTWKNVFRGHSIRALTMAPTDSK
                     ILVAGALDGVFLSTDAGKNWDQISPTNHVELRNIESVAVDPRDTKAIYAGTWHLPWKT
                     TDGGQSWFSTKGTETQILDDSDIFSIHIDRSNPDRVFSSACSGIYRSLDAGKNWTKFQ
                     GIPYSARRTHIIFQNPVYPDTIFAGTTEGLWRTTDAGTTWKLVTSKETVINAIEIHET
                     TPDRVVIGTANSGVLVSDNGGETFVASNRGFVTRQVSSIAVDPMQPGRLLASVLYNGA
                     EGSVYLSNDNGQSWYPSSQGIGPKDVYSLTINPGNTSQVYAATSNGVFLSENRGVSWK
                     RLEMKKVPPPPAPRSKAKTPAKSKKAASKGKKQSSTRNTAAKQSTSAKSKKSQPAPAK
                     SKAPATSDQTKIAKAAGSPFQDLDGHVMELSVTGDGRNGLVAATWEGLFRTYDPSKGW
                     EKLNVTGYTGRFFAVATTPHEPDTLLVGTSKGLYASLDQGKTWTQIKVDPDDNSFVQE
                     IAINPRDPKMILVGTRHSLYLTRDGMRNWDRLGHGIPYGEIASVRFNPRNPDEILVGD
                     ASQGGLYLSTDGARKFRRIDQKELPSHRVWAVAFDLFRDNHFYAGLFSSGVVVGTATA
                     PPSAGQP"
     gene            3815..4453
                     /locus_tag="PKZ53_02005"
     CDS             3815..4453
                     /locus_tag="PKZ53_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNJ39234.1"
                     /translation="MPLRVLLNISLLVAGSLILFWAEPGVAQQQSPVLTSNVKKPVAV
                     PATATPGHHQAQKISSSEIAHFYKVTDRLYRGGQPSDKGFEELKKLGITHIISLRSGS
                     DDAKIQNDETLTFKHIPVSVNPFKKPKEADVIQFLKAVTTTSNHPVFVHCREGVDRTG
                     LMIAAYRIVIDEWSREEAITEMNSIGSHWYYQRFESYLRKLDVEKIKKQINQ"
     gene            4729..>5617
                     /locus_tag="PKZ53_02010"
     CDS             4729..>5617
                     /locus_tag="PKZ53_02010"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39235.1"
                     /translation="MSIVAEVPTLFPVTAEELYAEVAVAAAVHQTYTYRIPPGLHHLA
                     QRGCRVAIPFGKSVQVGYVVALHDAPDESVPVERIKDIEEWLDETPLLTEEILDLTHW
                     VADYYYAPWGEVLKSAIPSGLQAVPHVRITITAHGRHELEEAQAAQRHHLTKWKFLTW
                     LTEVGGTALIENAPSDYSQNRVKALVRELEKAGFLQVQQALGKRRVQAKRQTMVQVPP
                     VIPADLELSEPAKPQKRTEAQERVLATLNEAGEPVLLPALTEKAGVSPAIVRTMAQKG
                     MVELVIREVRRDPLALLKAL"
BASE COUNT         1370 a         1480 c         1432 g         1335 t
ORIGIN      
        1 gcctttctgc caccagcgac ccgaaccctg gccggtggtc tatttatcgt tgatcgctcg
       61 attgatctgg tgaccatttt gatgctggcc ctggctggtt cagccggagt cctcaccgtt
      121 ggctggcaaa tcatcctggg aacaacctta ctcttcctgg tcggtggcgt cctgctctgg
      181 cccattttgc tcccgtggat gctgggctgg ctgccccttc cggttaaact ccgtcgaatc
      241 ctgtcagaac tggtgacggg acttcaggcc ctcagactcc gtgatctggg ctggaatttg
      301 tgtctgggtt gtgcgctgat ggtactcgat gtgatctcgc tctatgtgct ggtggccacg
      361 tttgaaccag tcagtttcaa agccgtgacc tttgcctttc ccttgatttt attgaccaac
      421 ctggtcccca ttacacccgg cggaatcgga gttcgggaaa gtgcctcggt gtttttgttc
      481 cgccagtttg gcgtgagtca ggcagcggct atttactcgg cgcttttcct gtatattctg
      541 aattcgctcc tgcctgggtt gtggggcatt acctatgtcc ggaaactggg agcccatccg
      601 tcagcggttg attcctctcc accagaaaaa attagcgagg tcacactgtc atgaatttga
      661 tggggaaaac agtactcatc accggcgggg cgcgtcgcct tggtcgggca attgcgcttg
      721 atctggcgac gcacggagcc aatatcgcct tgcattttcg atcttttccc gaagaagcct
      781 ttgcgaccct tgatcaatta cacaaatgcg gcgtacaggc cgccgccttt caggccaatt
      841 tgcttcaatc ggaagaactg acggatatga ttgctcaggt gtatcgaacc tttggccaga
      901 ttgatgtcct gattaataat gctgccgtct ttgaccgcaa atcatttttt gagcttaccg
      961 aaactgattg ggatcataca cttgacacga atttgaaaat tccattttta tgctcccggc
     1021 tggtcggaga aaaaatgctc ggtcaaggca ctggaaaaat tattaatctg gcctgcgtgg
     1081 gcggagtgcg cccctgggca aactttatcc cctattcagt ctccaaagca ggactgatta
     1141 tgttaaccga agccctggcc ctggccctgg ccccattgat ccaggtcaac gccatcgccc
     1201 caggcagaat tgagtttcct gatcacgctg tccccgacgt gccatcattt ccgactggcc
     1261 agcctcaaga catcacccgt gcggtgcgct acctgattga atctgattat gtgaccggtg
     1321 aaaccctttt ggttgacggg ggacgtcatt taagatgaga aagctgggcg aaggagaaaa
     1381 agatcctttc tttttagata gttatttatt gacagaaaaa ctacctggac ctgacactca
     1441 cgtacttcgt tcgttcacaa cctcgcgact tcgctcgttc accacttcac ccaaacaaca
     1501 ccatgaaaag aacttatttt acaactgtat tgtgtctgct tctgacgctg gggatggcct
     1561 ggtcgccagt actcttttca gccagtgcga tgggatctga ccctgattcc aattcgacga
     1621 cggcaaacat tgattttgaa tgggaactca atggcccatt tggcggcacg gttcgttcgc
     1681 tggttcaaaa tgatgccaac ccccaacaat tttttctcgg tacctcagat gggcaactct
     1741 atgtctcaaa tgatggcacg caaaactgga cctggattcc aacttttaat aaatccggga
     1801 acctgattga caatctggtg attgacccac gcgacccaga tacggtgtat ttgggaatgt
     1861 gggcgttggg tgaaaaacgc gatggggcga tttataaaag tacggatagt ggaaagacct
     1921 ggaaaaatgt ttttcggggc cattcgatcc gggcgctgac gatggcgcca accgattcaa
     1981 agatactcgt tgccggggcg cttgacggcg tatttttgag caccgatgcg gggaagaatt
     2041 gggatcagat ttctccgacc aatcacgtgg agcttcgcaa tattgaatcc gtagcggttg
     2101 atccgcgtga taccaaagcc atttatgccg gaacctggca tcttccctgg aaaaccaccg
     2161 atggtggcca gagctggttt tcaaccaagg gcacggaaac tcagattttg gatgattctg
     2221 atatttttag tattcacatt gatcggagca acccggatcg ggtgtttagc agtgcctgta
     2281 gcggcattta ccggagcctg gatgccggaa agaactggac caagttccag ggaattccat
     2341 acagcgcccg ccggacgcac attatttttc agaatccagt ctaccctgac accatttttg
     2401 ctggcacgac cgaagggctc tggcgaacca ccgatgctgg cacaacctgg aagctggtga
     2461 cctcgaaaga aaccgtgatc aatgcgattg agatccacga aacaacgcct gatcgggtgg
     2521 ttattggcac ggccaacagt ggtgttctgg tcagtgacaa tggcggggaa acgtttgtgg
     2581 cgtctaatcg cgggtttgtt acccgacagg tttcaagcat tgcggttgat ccgatgcagc
     2641 cgggccggtt actggcgtcc gtgctctaca atggcgctga aggcagcgtc tatctctcaa
     2701 atgacaatgg tcaatcgtgg tatccgtcct cacagggtat cggaccaaag gatgtctact
     2761 cactcaccat caatcctgga aatacgtcgc aggtctatgc tgccaccagc aatggcgttt
     2821 ttctatccga aaatagaggc gtaagctgga agcgattgga gatgaaaaag gttccgcctc
     2881 cacccgcccc acgttcgaaa gccaaaactc cggccaaatc aaagaaggca gccagcaagg
     2941 gtaaaaagca aagttccacc aggaataccg cggccaaaca atcaacctca gccaaatcta
     3001 aaaaatctca gccagctcca gccaaatcca aagcaccggc gaccagtgat cagaccaaaa
     3061 tcgccaaagc cgccggcagc ccgtttcagg atctggatgg acatgtcatg gaactttcgg
     3121 tgactggcga cgggcgcaat ggtctggtgg ccgccacctg ggaaggactc ttccgcacct
     3181 atgacccatc caaaggctgg gaaaaactca acgtaaccgg ctacaccgga cgcttttttg
     3241 ctgtggcaac caccccccac gaaccagaca ccctgctggt cggtacttca aaagggcttt
     3301 atgccagcct ggatcaggga aagacctgga cacaaattaa agttgatcct gacgacaatt
     3361 catttgtcca ggaaattgcc atcaaccccc gtgaccccaa aatgattctg gtcggcacac
     3421 ggcactcgct atatctgaca cgggatggaa tgcgcaattg ggatcggctt gggcatggca
     3481 tcccctatgg ggaaatcgcc tcggttcgct ttaacccacg caacccggat gaaatcctgg
     3541 tgggtgatgc cagtcagggc gggctctatc tctcaactga tggggcaaga aaattccgcc
     3601 ggattgatca aaaagagctt ccaagccatc gcgtatgggc cgtggcattt gacctgtttc
     3661 gcgacaatca cttttatgcc ggtttgtttt cgtccggtgt ggtggttgga acagcaacgg
     3721 caccaccatc tgccggccaa ccttaaacac cagaaccgca cccagaagct gtccccactt
     3781 tttttaacag ttcgtcaacc aaacgggggt ttgtatgcca ttgcgggtgc ttttgaatat
     3841 aagcttgctg gtcgcaggga gcctgatcct gttttgggct gagccaggtg ttgcccaaca
     3901 gcaatcacca gtcctgacct caaatgtcaa aaagccggtt gcggtcccgg ccacggccac
     3961 accaggtcac catcaggctc agaaaatttc cagttcagag attgctcatt tctataaagt
     4021 gactgaccgg ctgtatcgcg gcggtcaacc atcagacaaa gggtttgaag aactcaaaaa
     4081 actggggatc acccatatca tcagccttcg ctctggaagc gatgacgcca aaatacagaa
     4141 tgatgagaca ctgaccttca aacacattcc ggtcagtgtg aatccattca aaaaacccaa
     4201 agaagcggat gtcatccagt ttttgaaagc cgtgacgacg acttcaaacc acccagtgtt
     4261 tgtccattgc cgggaaggtg ttgaccgcac tggacttatg attgcggcat atcgaattgt
     4321 cattgatgaa tggagccgag aagaggcgat taccgaaatg aacagtattg gctcgcactg
     4381 gtactatcag cggtttgaat cttacttgcg caaactcgac gtcgaaaaaa tcaaaaaaca
     4441 gatcaatcag taagaccagt cagtagctgg cggtcagtag tcagtagttc actaagtttg
     4501 tttggttgaa tgatttggct gttttccaat acgaaaattt cattacaccc tggcatacaa
     4561 tgggcacccc agatttgggc gcggcagact tgaccttgca aaaaaacgaa accaatttat
     4621 aattactccg taatttttcc agtccattgg ttttaggctg gcacgaatgc cacctgttga
     4681 ggttaacttg cacccaaccg ttcaactctg gatgttatgg aaaattgtgt gagtattgtg
     4741 gctgaagtcc caacattgtt tcctgtcact gccgaagaat tgtatgccga agtcgcggtc
     4801 gcggcggcag ttcatcaaac atacacctat cggattccac cgggcctgca tcatctggcg
     4861 caacgcgggt gccgcgtggc catcccattt ggaaaatccg ttcaggtcgg ctacgtcgtg
     4921 gcattgcacg acgcgccgga tgaaagtgtg ccggtcgaac gcatcaaaga cattgaagag
     4981 tggctggatg aaacaccgct gctgactgag gaaatcctgg acctgaccca ctgggtggca
     5041 gattactact atgccccgtg gggagaagtg ctcaaatctg cgattccaag cggcttgcag
     5101 gctgttccgc acgttcgaat taccatcact gcccatggcc ggcatgaact tgaagaagct
     5161 caggctgccc agcgccacca cctgacgaaa tggaaatttc tgacctggct taccgaagtt
     5221 ggtggcacgg cactgattga aaatgcgccc tcagactact ctcaaaaccg ggtcaaagcg
     5281 ctggtgcgtg aactcgaaaa ggcagggttt ttgcaggtgc aacaggccct tgggaaacgc
     5341 cgcgttcagg ccaaacgcca gaccatggtt caggtaccgc cagtgattcc ggctgacctg
     5401 gagctttcag aacccgccaa acctcaaaaa cgcactgaag cccaggaacg ggtactggcg
     5461 acactcaacg aagccggtga accggtgctg ctcccggcat taacggaaaa agctggggtc
     5521 agcccggcca ttgtccgcac catggcccaa aaaggaatgg ttgaactggt cattcgtgaa
     5581 gtccggcgcg acccgctggc cctcttaaaa gcccttc
//
LOCUS       DAOQDO010000073         5615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_8976_length_5615_cov_2.975180, whole genome shotgun sequence.
ACCESSION   DAOQDO010000073 DAOQDO010000000
VERSION     DAOQDO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8976_length_5615_cov_2.975180"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..3112)
                     /locus_tag="PKZ53_02015"
     CDS             complement(53..3112)
                     /locus_tag="PKZ53_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39236.1"
                     /translation="MWPWAGSGLMSMLLWCLLTVPCTAQTYRFDHWTTNEGLPQNHIT
                     AIVQTQDGYIWFTTFDGLVRYDGIKFKIFDRKTDRAIPSNRFTALFEDAQGTLWAGTE
                     DAGLVCYRNGVFTAFTQADGLPQSWITGIQADTNGRIIVATPRAMVYWDNGKFFPVTH
                     QIAMFSSLVGQSGTRWVVDETGVHWCRNGDCHDLFLPFYKQYSSILGNRPYLYEDRDG
                     NLWICSIGNKLVQIHNGRLIEYDLTNRELLNAPTDRNPKLKELFVIAATQDLTGNVWF
                     GTTNGLLRFQNDRFSRYTTQHGLSSNMMTGLMTDREGIVWVGTHDQGLNRVTQQFITT
                     LSVEEGLPDREVYPILQDRAGQIWVGSINLSKFDGTKFTVYKTGFADINNQIQALYED
                     TQGRLWIGAYGGICYFEQGQFFDFSDRMFATLPQQFHRPNCNAIFQDSQQRLWFGTDR
                     GLFKYEQNQITPYTVDDGLPSNTIKALFLDRQGTFWVGTYGGAAYLDGNRWISLTERD
                     GLASNKVRTITQDSIGNIWIGTYDGGISRYKDGKLSTCTVENGLFSNGVFAILEGRRD
                     NFWMSSNQGIFRVNKQELNDFADGKRSSVTSVAYGRLDGMRNTECNGGRQPAGVKTRD
                     DKLWFPTMDGVAVVDLNTQPINHLPPQVLFESAVIDKTEFPITDRIQLQPGQTYLEIN
                     YTGLSFTKPEQVQFKYKLQGLDPDWINVGTRRTAYYSYVPPGTYRFTVLAANSDGIWS
                     QTGPELIVEIIPPVYRRRWFQALIALGLAGCGWGVYRLRIAQLQREHHLREAFSQQLL
                     ASQEAFSRELMTSQEQERKRIAAELHDSIGQSLLVIKNRALLGLKTSPDPKTREQLEE
                     INGSVSEALAEVRSVAYNLRPIHLERLGLTSTLEEMIETVSEASGIEIHPDIAPLDDL
                     FSNEDEIHFFRILQECLNNIVKHSQATEVELTIYREPTGVAVTVQDNGKGFSDPPQSN
                     SKRNGSQSGFGLTGIAERVRMLGGHFEIHSIPNHGTTVSIQILRTSG"
     gene            complement(3501..>5615)
                     /locus_tag="PKZ53_02020"
     CDS             complement(3501..>5615)
                     /locus_tag="PKZ53_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNJ39237.1"
                     /translation="EMIGEQPQSARSNYLFGNKASQWKRDIPHFGKVRYHCVYPGIDV
                     VVYGNPTQLEYDFVVSPGSQADAIVLGFEGIDQLSLDQRGNLMVTGNSTTTIHHKPVA
                     YQMISGQKRLVDVEYQLDFPQPPAHLLQPPTPNQVRFKLGDYDPTQPLVIDPTVSFSS
                     FLGGGFPDAAFAVAADGLGNVVVVGTTLSINFLTQSPLQPENAGEADMIVAKIDTVSG
                     QLVFSTYLGGDRFDEARGVAIDRDGGIYIAGRTTSLVFPTTDQAFQKENKGNSDGFVV
                     KLNSDGSAVEFATLLGGAGRDEIRGLALDAQGNPSVTGFTLSTDFPVKNAFQPRDGGG
                     SFTDAFVTKLNRTGSELIYSTYLGGNEDDLGADIAVDQKDGSAVVTGYLNSKNFPLKN
                     PTQATFSGGLYDVFVTKFSPAGDALVFSTYLGATDQERGAAAGVDEFGDVYVTGFTGS
                     TDFPTTANALQKDLRGVSDIFVTKYGPTGGRIFSTFFGGAKDEQPFDMAIDARNTIYL
                     VGVTTSLDYPLVNALRTEPKNVDSFVTRLSRTGDRAIYSTYFGGAQVDNIFGVAADAD
                     GNAYVCGQTTSDDLPLVNPVQPKRAGVGDIFISKVTDPADFTLNIALSSVSIRRGQKI
                     KLEFTINRFGGYDDLVTVTGPAVKEFGLKLKPGVVITPESQAVFTLKAKATAQQATRM
                     LTFTATDNFGRTRTLPIMVTIQ"
BASE COUNT         1327 a         1452 c         1465 g         1371 t
ORIGIN      
        1 tttaatacct tgatcctgac ttcttcagcc cgaagttttc agcccgaagt tttcagcccg
       61 aagttcgaag gatttgaatt gaaaccgtgg tgccgtgatt gggtatagag tgaatttcaa
      121 agtgtccgcc gagcatcctg acgcgttcag cgataccagt caaaccaaat cctgactgtg
      181 atccattccg ttttgaattc gattggggcg gatcagaaaa cccttttcca ttatcctgaa
      241 ccgtaaccgc aacccctgtt ggctcacgat aaatggtcaa ttcaacttcc gtcgcctgtg
      301 aatgcttgac gatattattg aggcattcct ggagaatccg gaagaagtgg atttcatctt
      361 cgttggaaaa taaatcatcg agcggggcaa tatcaggatg gatttcaata cctgacgctt
      421 cgctcacggt ctcgatcatt tcttcaaggg tggaggtcag ccccaatcgt tcgaggtgaa
      481 tcgggcgcag gttataggcg acgctgcgga cttcggccag ggcttcgctg acggagccgt
      541 tgatttcttc gagttgctct ctggtttttg ggtctggtga agttttcaaa cccaggagcg
      601 cccggttttt aatgaccagc agactttgcc cgatgctgtc gtggagttcg gcagcaatgc
      661 gcttgcgttc ttgctcctgc gatgtcatca gttcgcggga aaaagcttcc tgtgaggcga
      721 gcagttgctg ggagaaggct tcgcggaggt ggtgttcacg ttggagttga gcaatccgca
      781 ggcgatagac tccccatcca cagccggcca gcccaagagc aatcagcgcc tgaaaccagc
      841 gtctccgata gacgggcgga ataatttcca caatcagttc cggcccggtt tgactccaga
      901 taccgtcgct attagctgcc aggaccgtga agcgataggt ccccggcggc acataggaat
      961 aataggcggt gcggcgtgtg ccgacgttga tccaatccgg gtctaatccc tggagtttgt
     1021 atttaaactg aacttgctcg ggtttggtga aactcaggcc agtgtaattg atttcaaggt
     1081 aggtttgccc tggctggagc tggattcggt cagtgattgg aaattcggtt ttatcaatta
     1141 cggctgattc aaacagcacc tgtggcggca ggtgattgat tggttgagtg ttgaggtcaa
     1201 ccactgccac accatccatg gttggaaacc agagtttgtc gtcacgggtt ttcacaccgg
     1261 ctggctggcg tccgccgttg cactcggtat ttcgcatgcc atcaagtcgt ccataggcaa
     1321 ccgaggtcac ggatgagcgt tttccgtcgg caaagtcatt gagttcctgc ttgttgactc
     1381 gaaaaattcc ctgattggaa ctcatccaga aattgtcacg gcgaccttcg aggatggcaa
     1441 agacaccatt actaaagagg ccgttttcaa ctgtgcaggt actcaatttt ccatctttgt
     1501 agcgactgat gccgccgtca taggtgccga tccagatgtt gccgatgctg tcctgggtga
     1561 tggtgcggac tttattgctg gccaggccgt cacgctccgt gagtgaaatc cagcggttcc
     1621 catccagata ggccgctccg ccgtatgttc cgacccaaaa tgttccctgg cgatcaagaa
     1681 agagagcttt gatcgtattg ctggggagtc cgtcatccac agtatacggc gtgatctggt
     1741 tctgttcgta tttgaacaaa ccccgatccg tcccaaacca cagccgttgc tgggaatcct
     1801 gaaaaatggc gttgcagttc gggcgatgaa attgttgggg cagcgtggca aacattcgat
     1861 ccgaaaaatc aaagaactga ccctgctcaa aataacaaat gccaccatag gcgccaatcc
     1921 acagccgacc ctgtgtatct tcatacaggg cctggatttg attgttgatg tcggcaaaac
     1981 cagttttgta gacggtaaac tttgtgccgt caaatttgga caggttaatc gagccaaccc
     2041 atatctggcc ggcgcgatcc tggagaatcg gatagacttc acgatctggt agcccttctt
     2101 cgacggaaag cgtggtaata aactgttgcg tcacccgatt gagcccctga tcatgcgtgc
     2161 cgacccagac gattccttcc cggtcagtca tcagaccagt catcatattg ctcgacagtc
     2221 catgctgagt ggtatagcgg ctaaagcgat cattctgaaa tcgaagtaat ccattggtcg
     2281 ttccaaacca tacatttccg gtcaggtctt gggtggcggc aatcacaaag agttctttca
     2341 atttgggatt gcggtcagtc ggtgcgttga gcaattcgcg gttggtcagg tcatactcga
     2401 tcagccggcc attatgaatt tgcaccagtt tgttgccaat cgaacaaatc cataaattgc
     2461 cgtcccggtc ttcatacaaa tagggccgat tgccaagaat tgacgaatat tgcttataaa
     2521 atggcaagaa gaggtcgtga caatcaccat ttcgacacca gtggacccct gtttcatcaa
     2581 cgacccatcg cgtaccgctt tgacccacca gcgaagaaaa catagcgatc tgatgtgtga
     2641 cggggaaaaa cttcccattg tcccaataga ccattgcccg tggtgttgcc acgataatcc
     2701 ggccattggt gtcagcctga atgcctgtga tccagctttg cggtaatcca tcagcttgag
     2761 tgaaggccgt aaacaccccg ttccgatagc aaaccagtcc ggcatcttcg gttcccgccc
     2821 acaatgtgcc ctgggcatct tcaaagagtg ccgtaaaccg gttgcttgga atcgcacggt
     2881 cggtctttcg gtcaaaaatt ttgaatttga tcccgtcata acgcaccagc ccatcaaatg
     2941 tagtaaacca gatgtacccg tcctgggtct ggacaatggc ggtgatgtgg ttttggggca
     3001 gaccttcatt cgttgtccag tggtcaaacc ggtaggtttg ggcagtgcag ggcactgtca
     3061 ggagacacca cagcaacata ctcatcagcc cggagcctgc ccagggccac aagccgtgca
     3121 ctctcaagag tttctgccaa acaaataacc gctgcccgcg caccgtcatg aaccacctgt
     3181 tgatttcact ttgggagaag aggattggga actgcccgag tgtgacgcaa ccagggttgg
     3241 gaggcaagga aaaccagggc tgaagacgtc gggctgaaga cgtcaggcca taagcgccag
     3301 gataagaaaa accgtgtcct ttccgttggc attgaacggt gcttttgttg ggcctgggcc
     3361 tcgtcctggc cgtgaccgtg ggctttgcac cacggctatt gaacgacgac ccttcgggcc
     3421 tcaaatcctt attctggcgc gcttatgggg cttggggctg agggcttggg gctgaagaac
     3481 tggttttctt tcatccctct tcactgaatt gtcaccataa tcggaagcgt tcgggtgcgg
     3541 ccaaaattat cggtggcggt gaaggtcagc attcgggtgg cctgctgggc ggtggctttg
     3601 gcttttaagg taaacacggc ctgcgactct ggggtgataa ccacgcccgg tttgagcttg
     3661 agcccaaact ctttcaccgc cggtccagtc acggtgacca ggtcgtcata gccgccaaag
     3721 cgattgatgg taaattcaag tttgattttc tgaccacgtc ggatactcac ggatgaaagt
     3781 gcgatattga gggtaaaatc tgccggatca gtaactttac tgataaaaat atcgccaaca
     3841 ccggcccgct ttggctgcac cgggtttacc agcggcagat cgtcagacgt tgtttgaccg
     3901 cacacatagg cattgccgtc agcatcagcc gccacgccaa aaatgttgtc aacctgggca
     3961 ccgccaaaat acgtcgaata gattgcccgg tctccggtgc gactgagccg ggtcacaaaa
     4021 ctgtcaacgt ttttaggttc agttcgaaga gcattcacaa gcggatagtc aagtgaagtg
     4081 gtcactccga ccagataaat tgtgttgcgg gcatcaatgg ccatatcaaa tggctgttcg
     4141 tctttggcgc ctccgaaaaa ggttgaaaag atgcgccctc cggttgggcc atatttggtg
     4201 acaaagatat ctgacacgcc acgcaggtct ttttggagcg cattcgccgt ggtcggaaaa
     4261 tcagtcgaac cagtaaatcc agtgacatag acatctccaa attcatccac cccagccgct
     4321 gcgccgcgtt cctggtcagt cgcgcccaga tacgtcgaaa ataccagagc gtcaccggct
     4381 ggactgaatt tggtcacaaa cacgtcgtac agaccgccgc taaatgtcgc ctgggttgga
     4441 tttttcaggg ggaaattttt cgaattgaga tacccggtca ccaccgcgct cccgtctttt
     4501 tgatccacag caatgtccgc cccgagatca tcttcatttc cgccaagata ggtcgaatag
     4561 atcaattcgc ttccagtgcg attcaatttg gtcacaaagg cgtcagtaaa cgagccgcct
     4621 ccgtcacgtg gttggaacgc attcttcacc ggaaagtcag ttgagagggt aaacccggtg
     4681 acagatggat ttccttgagc gtccagcgcc aggcctcgaa tttcatcccg accggcccca
     4741 cccaacagcg tggcaaactc aacggctgat ccgtcggaat tcagcttaac aacaaaaccg
     4801 tcactgttgc ctttgttttc tttttgaaat gcctgatctg tggttgggaa caccagtgac
     4861 gtggttcgcc cggcaatata aataccgcca tcccgatcaa tggcaacccc acgtgcttcg
     4921 tcaaagcggt cgccgcccag gtaggtcgaa aagaccagtt gtcccgatac ggtatcaatt
     4981 ttggccacaa tcatgtcggc ctctccggca ttttccggtt ggagcggaga ctgagtcaga
     5041 aaattgattg acagtgtggt gccaaccacc acgacattcc caagaccatc agccgccaca
     5101 gcaaacgccg catctgggaa accgccgccg agaaaacttg aaaaactcac cgttggatca
     5161 atcaccagcg gctgggttgg atcatagtcg ccaagcttaa agcgaacctg gttgggggtt
     5221 gggggctgaa gaagatgggc tgggggttgg gggaaatcaa gctggtactc gacatccacc
     5281 agccgttttt gtccgctgat catctggtag gcaactggtt tatgatgaat agttgtggtt
     5341 gagtttccag tcaccatcag gtttccacgc tgatcaagtg aaagctggtc aatgccttca
     5401 aagccaagca ctatcgcatc agcctgactt ccaggtgaca ccacaaagtc atattcaagc
     5461 tgcgtcggat tgccatagac caccacatca atccccggat agacacagtg atagcggacc
     5521 tttccaaaat gcggaatatc tcgtttccac tgactggctt tgttgccgaa cagataattt
     5581 gaccgggcgg attgtggttg ttcgccaatc atctc
//
LOCUS       DAOQDO010000074         5601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9005_length_5601_cov_3.101154, whole genome shotgun sequence.
ACCESSION   DAOQDO010000074 DAOQDO010000000
VERSION     DAOQDO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9005_length_5601_cov_3.101154"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3370
                     /locus_tag="PKZ53_02025"
     CDS             <1..3370
                     /locus_tag="PKZ53_02025"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNJ39238.1"
                     /translation="ALRIVENMETSLSVPIATSQRQIPIKLLDENRRLMNQFLEQSGR
                     KVSFTHIIAYALVKALDRFPQLNAFFEVQDGTSYRVQCTNVNVGVAVDVTKKDGSRTL
                     LVPNIKAANKMDFAELVAAYDDVVKRARAGKLQVPDFQGTTISLTNPGTLGTTASNPR
                     LMAGQALIIATGAIEYPPEFLAMAPEAISRIGISKVMTITSTYDHRIVQGAESGSFLA
                     FVDELLRGKHRFYNTIFADLGIPYKPYRWAVDSNPAILGIDRHRDEVRKQASVLELIN
                     AHRVRGHLVADIDPLGAMQFQEHPELDIETYKLTIWDLDREFITGGLGNTEKATLRQI
                     LLWLRKYYCSKVGIEYRHIQNPEEKEWIRSQVERDHPPVPVEVKKQLLWKLISAEQFE
                     RFLGVKYLGQKRFSVEGAETIVALLDQVIESAPSRGIDEIVIGMAHRGRLNVIANVIG
                     HFCERIFTSFEGSVHPKFPHDQGDVKYHQGASGVRDTAHGKQVTVSVLSNPSHLEFVD
                     PVVQGVVRARQDQKMELATKSGILTPSDRYSKFAVLLHGDSAFAGEGIVPETLNMSQL
                     AGYSTCGTLHFIINNQVGFTTTAEDSRSSTYSTDVAKMVQAPIFHINGDDPEAAYHIL
                     QLALDYRQKYHKDIVLDVIGFRRHGHNEGDEPTYTLPIMYQRIRNHEGVRTIYSKRLI
                     REGVITAEGVDGLIADRVRRYENALLGAKEIVSRRDVKLVLPNPKEPEVIEEFSTSVN
                     QDVLKKLAHAATAVPSGFNINPKIVGLLTKRAKMVDGEIPVDWAMGESLAFGSILLDG
                     ITVRASGQDTVRGTFSQRHAVFFDAHTGEPWMPLTTLSPEQGQFHLYDSPLSEAGVLG
                     FEYGYSVAHPEALVIWEAQFGDFANAAQVIVDQFIAAGEEKWNQPTRLVLLLPHGYEG
                     QGPEHSSARLERYLQLCAGGNMQVVNCTNAAQYFHLLRRQVKMTHAKPLIVMTPKSLL
                     RHPGASSPITDFTQGTFQPVVMDPATSGLAKRVLLCSGKVYYELKAKLQELKDTQTAI
                     IRLEQLYPFPKALLLQLLNSPGLSQATDIRWVQEEPANQGAWTFVEPRLRQLLRQNQA
                     VRYVGLPASGTSATGSYIIHQMQQKQLLTESFEN"
     gene            3804..5009
                     /locus_tag="PKZ53_02030"
     CDS             3804..5009
                     /locus_tag="PKZ53_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNJ39239.1"
                     /translation="MTPRKSSSTASAMTATASKTADQLADRRAITLSIDEKLSFFTRL
                     AASVDSGKGIPGFLLSEVELYDLLQKTKPDSRAFLLSGRPSQGREQLVRELAHLMLEL
                     GVAFAPASQHFPGVFDPYLVAVLNAAEKTGDYGPVLRRYVKNLIDRKNMRDTLINAMI
                     YPALVLVVALIVIVLFMVVIVPGFSTIYEGLLGEAKFHWATQLVISTSDIMVGYWYLI
                     LAGVGAAVVAAWFGHKNVPQVKEFEHRAMLAIPVIGTLVAQVDAFNFLTTFTMLISAG
                     GLLTESSELAADAIINSELRDAARKGSDHFTSGRTHSLAVAFASEHPVFSPASSFFNE
                     MKTFEDTGRLEVLEKYADNLKYNADQTRDRLVKLIEPVSLLMLGGLVGFLVFALYMPM
                     FELVGKLAQ"
     gene            complement(5083..5586)
                     /gene="purE"
                     /locus_tag="PKZ53_02035"
     CDS             complement(5083..5586)
                     /gene="purE"
                     /locus_tag="PKZ53_02035"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNJ39240.1"
                     /translation="METKEKSFPLVGIIMGSDSDLPTMKEAVAVCEEFEVPYEVRIVS
                     AHRTPRDMVLYAESAHIRGLQVIIAGAGGAAHLPGMVASLTPLPVIGVPIHSRSLNGL
                     DSLLSIVQMPSGVPVATVAIGAGRNAGILAVQMVAMTNKELQQEILDFKAKMAEISRS
                     KNHTLPH"
BASE COUNT         1343 a         1396 c         1465 g         1397 t
ORIGIN      
        1 ggcgttgcga atcgttgaaa acatggaaac cagtctttcc gtgccgattg ctacctccca
       61 gcgtcagatt ccaatcaagt tgctcgatga aaaccggcgg ttgatgaatc aattccttga
      121 gcagtcaggc cgcaaagttt catttaccca tatcattgcc tatgctctgg tgaaggccct
      181 ggatcggttt ccgcaactca atgctttttt tgaagtccag gacgggacat cgtaccgggt
      241 gcaatgtacc aatgtgaatg ttggtgtggc ggtggatgtc accaaaaaag acggttcccg
      301 gacattgttg gtgccaaaca tcaaagcggc caacaaaatg gattttgccg aactggttgc
      361 ggcctatgat gatgtcgtca aacgtgcccg ggctggaaaa ctgcaagtgc cagattttca
      421 gggcaccact atttcgctga ccaatccagg aacactcggc accacagcgt caaatccccg
      481 gttgatggcg ggtcaggcac tgattattgc gacgggcgcc attgagtatc cgccggagtt
      541 tctggccatg gcgcctgaag cgatttcacg catcggaatc agcaaagtca tgacgatcac
      601 cagcacctat gatcaccgca tcgtacaggg tgctgaatcc ggatctttcc tggcatttgt
      661 tgatgaactg ctgcgcggga aacaccgctt ttacaacacg atttttgcgg atttgggcat
      721 tccttataaa ccctatcgct gggcggttga cagcaacccg gctattttag gtattgaccg
      781 tcatcgggat gaagtccgca aacaggccag cgtgctggaa ctcatcaatg cccatcgtgt
      841 tcgcggccat ctggtggctg atattgaccc gttaggcgcc atgcaattcc aggaacatcc
      901 ggaacttgat atcgaaacct ataaactgac aatttgggat ctggatcgcg agttcatcac
      961 gggtggattg ggcaataccg aaaaagcgac gttgcgccag attctgctct ggttgcggaa
     1021 gtattattgc agcaaggttg gtattgaata tcgtcacatt caaaatccag aagaaaaaga
     1081 atggattcgc tcgcaagtcg agcgtgatca tcccccggtc ccggttgagg tcaaaaaaca
     1141 actgctctgg aagctcattt ccgccgagca atttgagcgt tttctcgggg tgaaatacct
     1201 gggacagaaa cggttttcgg tcgagggcgc tgaaaccatc gtggccttgc tcgatcaggt
     1261 aattgaaagt gccccgtcgc gtgggattga cgaaatcgtc atcggcatgg cccaccgtgg
     1321 tcgcttaaat gtgattgcca acgtgattgg tcatttctgt gagcggatct ttaccagttt
     1381 tgaaggttcg gttcatccaa aattcccgca cgatcagggt gacgtaaaat atcaccaggg
     1441 cgcatcgggt gtccgggata cggctcatgg caaacaagtc accgtgtcgg tgctgtccaa
     1501 tccaagccac cttgagtttg tggatccagt ggtccagggc gtggttcggg ccaggcagga
     1561 tcagaagatg gaactggcga ccaaatcggg catcctgacc ccatcggacc gctacagcaa
     1621 atttgctgtt ttgctgcacg gagattcggc ttttgccgga gagggaattg tccctgaaac
     1681 cctcaatatg tcccaactgg caggatatag cacctgtgga acactccatt tcattattaa
     1741 caatcaggtt gggtttacca ccacggctga agacagccgg tcctcaacct acagcaccga
     1801 tgtcgccaaa atggttcagg ctccgatttt ccacatcaat ggtgatgatc cagaagccgc
     1861 ttaccatatc ttgcaactgg ccctcgatta ccggcaaaaa taccacaaag acattgtgct
     1921 ggatgtgatt gggttccgcc gccacggaca taatgaaggt gacgaaccga cgtacacctt
     1981 gccaattatg taccagcgga ttcgtaacca cgaaggcgtg cggacgattt acagcaagcg
     2041 attgattcgc gaaggggtga tcacggctga aggcgtggac ggactgattg cagatcgggt
     2101 tcgtcggtat gagaatgccc tgcttggcgc caaagaaatt gtgtcgcgcc gggatgtcaa
     2161 actggttctt ccaaatccaa aagaacccga agtgattgaa gaatttagca ccagcgtcaa
     2221 ccaggatgtg ctgaaaaaat tggctcatgc ggcaaccgcc gttccgtcgg gctttaatat
     2281 caatcccaaa attgttgggt tattgaccaa acgggccaag atggtggatg gggaaatccc
     2341 ggttgattgg gccatgggcg agtcactggc atttggttcg attctcctgg acggcatcac
     2401 cgtgcgtgcc agtggccagg acacagttcg gggtacgttt agccagcgcc acgccgtgtt
     2461 ttttgatgct cataccggtg agccctggat gccactgacc acgctttcgc ctgagcaggg
     2521 acaatttcac ctctatgata gtcccctttc agaagccggt gtgcttggat ttgaatatgg
     2581 atacagcgtc gcccatcccg aagccctggt gatttgggaa gcccagtttg gtgattttgc
     2641 caacgccgcc caggttatcg tggaccagtt tattgccgca ggtgaagaaa aatggaatca
     2701 accaacccga ttggttctac tcttgcctca cggctatgaa ggtcaagggc cagaacactc
     2761 cagcgcccgc ctggaacgct atctccagct ctgtgccgga ggcaacatgc aggtcgtcaa
     2821 ctgtaccaat gccgcccagt atttccattt gttgcgtcgt caggtcaaga tgacacatgc
     2881 caaacccctg attgtgatga caccaaagag tttgttgcgt cacccaggag cctcatctcc
     2941 gatcactgac tttacccagg ggacgttcca gccagtggtg atggacccgg cgaccagtgg
     3001 tctggccaaa cgggttttac tgtgcagcgg caaggtgtat tacgaactga aagcaaagct
     3061 ccaggaactc aaagataccc aaaccgccat tatccggctt gagcaattgt atccgttccc
     3121 gaaagcctta ttgctgcaat tgttgaattc gccagggttg tctcaggcaa ctgatattcg
     3181 gtgggttcag gaagagccag cgaaccaggg tgcctggacc tttgtcgagc cacggttgcg
     3241 acaattgtta cggcaaaatc aagcggtgcg gtatgttgga ttgcccgcca gtggaacttc
     3301 cgcgaccggg tcctacatca ttcaccagat gcagcaaaaa caacttttga ccgaatcgtt
     3361 tgaaaattaa aagtcagtca cactcaaaaa ttccagcctg gcctggtttc gagcagtgga
     3421 ctcttgaacc aaagacagtt caacgcgttg cccgaaaacc agtgccgggt tggcacctgt
     3481 tccccaggaa atctccagat cagataccag aagattgagg attaggtcct atatcttcaa
     3541 gcgggtaccc aggctgatga attgtcagcc gtttctatcg cttctggtcc cagttatatc
     3601 ggttggatac gatcatgagg gaacacggtg cgccagagat ttttccaaga cctgaagaca
     3661 gggaactgcc accttgtttc gcctggatag atcgtcaagt tgttcaatga aaagaacctt
     3721 tgctaactgc tgaccatcaa ctggtagccc ccttgtctca atcatccaac ggccagatga
     3781 actttaagac caaggatcag gatatgaccc cccgtaaatc aagttcgact gcttctgcaa
     3841 tgacggccac cgcgtcaaag acagccgacc aactggctga tcggcgagcc attactctca
     3901 gtattgatga aaaactttcg ttttttaccc gcctggccgc ctcggttgat tctggaaagg
     3961 gcattcctgg gtttttactc agtgaggttg aactctatga cctcctccag aaaacaaaac
     4021 ctgattcacg cgcttttctg ttaagcggga ggcccagcca ggggcgtgaa caactcgtcc
     4081 gcgaactggc ccacctgatg ttggagcttg gagttgcttt tgcaccggcg tctcagcatt
     4141 tcccaggtgt atttgacccg tatctggtcg ctgtgctcaa tgcggctgaa aaaacggggg
     4201 actatggtcc agtcctgcgc cggtacgtga aaaacctgat tgatcgcaaa aatatgcgcg
     4261 ataccttgat caatgccatg atttatcccg cgttggtgct ggtggtggct ctgattgtga
     4321 ttgtgttgtt tatggtggtt attgtgccag gtttttcaac tatttatgag gggttgttag
     4381 gcgaagcaaa attccattgg gcgacccaac tggtgatttc aacctcagat atcatggttg
     4441 gatactggta tttgattctg gctggtgttg gggctgccgt cgttgctgcc tggtttgggc
     4501 acaaaaacgt gcctcaggtc aaggaatttg aacatcgagc catgctggcg attccagtga
     4561 ttggaacatt ggtggcccag gttgatgcgt ttaatttcct cacaaccttt actatgttga
     4621 ttagtgcagg tgggttgctg acggaaagtt cagagctggc cgccgacgcg attattaatt
     4681 ctgaactgcg tgatgccgct cgaaagggga gcgatcactt tacttctggg cgcacccatt
     4741 cactggctgt ggcctttgct tctgaacatc cggtattttc acccgcatcg agtttcttca
     4801 atgagatgaa aacgtttgaa gataccggaa gactggaagt gttagaaaaa tatgctgaca
     4861 acctcaaata caatgccgac cagacccgtg atcgcctggt gaagctcatt gaaccagtgt
     4921 ctttgttgat gctcggcggc ctggtcggat ttctggtctt tgccctgtat atgccaatgt
     4981 ttgaattagt tgggaaactg gcacagtgac cagtgactga ttgacagggg gatagctgac
     5041 agatggcact ccgtcaggtg ccactttgtc attctatcag gattaatgcg gcaaggtatg
     5101 gttcttgctc cgggaaatct cagccatttt ggctttgaaa tcgaggattt cctgttggag
     5161 ttctttgttg gtcatggcca ccatttgaac agctaagatc ccggcattgc gtccagcccc
     5221 aatcgcaact gtggccactg gaacgcctga aggcatttgg acaattgaga gcagcgaatc
     5281 aagcccattg agtgaccgac tatggattgg aacaccaatc acaggcagtg gtgtgagcga
     5341 ggctaccatt cccggtaagt gagccgcacc accagccccg gcaatgatga cttgcagccc
     5401 acggatatgg gctgattcag catagagtac catgtcacga ggcgtacggt gggcggagac
     5461 gattcgcact tcatagggca cttcaaattc ttcacagacc gccactgctt ccttcatcgt
     5521 tggcaaatct gagtcactgc ccataattat tcccaccaat ggaaatgatt tttcttttgt
     5581 ttccataatc ggattccccc t
//
LOCUS       DAOQDO010000075         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9073_length_5574_cov_3.172314, whole genome shotgun sequence.
ACCESSION   DAOQDO010000075 DAOQDO010000000
VERSION     DAOQDO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9073_length_5574_cov_3.172314"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="PKZ53_02040"
     CDS             <1..1960
                     /locus_tag="PKZ53_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39241.1"
                     /translation="TTRADWQVSNDDKMAFRYSLQRENDIDRGSLRAPIGTADNRQHS
                     FNNYQSFVYNWVHTFSPSLLNDFTFHENNFINRIPTFVENRNELRFPSVQDGGNFRIP
                     QRTRQNRIQFRDNLSWILGQHTVKFGGEYHRLDTDAIFDLFGSGTIFLAEDFASQDRN
                     RDGSLNDLDIPLVLALRSIAPNRPPTVPNVDNNFVAFYFQDDWKVRPNLTINLGLRYE
                     LDTDVKNISNFDQINPLVLPFLAPGGRSRNTKNFAPRVGFNWDPWSDGKTSIHGGYGI
                     YYDRVILEVQLLERLLDGRTLPLEARTGSALDASGNFVAGTPTLAAPFTGQIIPGAGA
                     VGINIIDNNLDTPYVQQFNFGFQREIMNGLTVSVDGVHSFGSKFIIGRAIGAVFNPVV
                     GGPDGIVNIESSVKNWYDGLLISVQKRHSNHFSFNASYTLSKTFTFSNDDQIPFQAGP
                     LDPNNLNLEKGPAPNDERHRFTLAALFDLPLGFQLSPIYTVTSGVPFDIVLPNGTTRI
                     PYIQRNAAGRQFKTGADLNEFITQINAGGGIAGVGLLPLVNDNLRFGDSFQSFDLRLT
                     KTFTIGDRLKVQGIAEVFNLFNVTNIRGVTNVNYSGFQNTLIRDSQDPNNPGFLRSSR
                     FGTPIQTAGGVFGTGGPRAFQLAVRVNF"
     gene            complement(2101..2757)
                     /locus_tag="PKZ53_02045"
     CDS             complement(2101..2757)
                     /locus_tag="PKZ53_02045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ39242.1"
                     /translation="MSSLLTYLRPLLSQNKLHFSELQLEQLAGHFDVLCDWNQRMSLT
                     TITNPEAAARLHYLDSLAAAPYLEALNTEVEVWVDVGSGGGFPGVPLSIMFHVEHFLL
                     TDSQHKKAQFLNEVIQNLGLESHVTVFEGRVEAGQLAALFGTEKIPRWGILARAIEKM
                     DKQLPKLLKLPGLQVAFLWVGGDTAQTLSQNPHRGWTVETVKLPSGDQRYLVKFRKQI
                     "
     gene            complement(2900..4792)
                     /gene="mnmG"
                     /locus_tag="PKZ53_02050"
     CDS             complement(2900..4792)
                     /gene="mnmG"
                     /locus_tag="PKZ53_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNJ39243.1"
                     /translation="MSVEYCFNEAFDVVVIGAGHAGCEAAHAAAQLGCTTALITLNLD
                     LIAQMSCNPAIGGIAKGHLVREMDALGGLMGQIIDRTGIQFRLLNRSRGPAVQSPRAQ
                     ADRGLYRTEMRRYLETVPNLHLRQGEVIDIMVEQDRIIGVEMWDGRKIATKALVVCTG
                     TFLNGLIHIGPRTYSAGRSGELPSILLAASFRKLGLRVGRLKTGTPPRINSRTIDFSN
                     LEEQPGDSTPVPFSFQTQKVVQPQLSCYITYTTPDTHRIIRENLDKSPLYSGQIQSTG
                     PRYCPSIEDKIVKFADKDRHQIFLEPEGHHTNEVYVNGLSTSLPIDVQEKIVRSIPGL
                     EKAQLIRPGYAIEYDFVDPTELRPTLEVKRVRGLFHAGQINGTTGYEEAGCQGLIAGM
                     NAGLMALGREQVTLDRAKSYVGVLIDDLITSGVDEPYRMFTSRSEVRLLLRIDNTDQR
                     LTPLGRQVGMVNDEQYQAFLARQERVNALHTLLEKTPLSPTSEVYETFRQTTGGTQIR
                     ETTRLATLARRPEVTIEAIAGVLRQLGHENFIEEELGVAVNDLKYAGYVKHQESTAQK
                     MAKAAEVRIPTDFVFRGIPGLSREMSEKFSRVRPETIGQAGRIPGVTPAAIAILSMHL
                     EIHRRG"
     gene            5069..5503
                     /locus_tag="PKZ53_02055"
     CDS             5069..5503
                     /locus_tag="PKZ53_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39244.1"
                     /translation="MNTQELSARVVQQLPVETQELLFPLISELRALPIETQLDFLADL
                     HKELSQRKVQSQSVLEAIISPSLPDEDQEVRATKRQQREEWLKANYADYAGKYIALDG
                     NRLLGVGQNYAQVRKAALQAGVKDAYIDFVHPPNAKGFMGGW"
BASE COUNT         1364 a         1477 c         1396 g         1337 t
ORIGIN      
        1 gaccacgcgg gctgactggc aggtgagcaa tgacgataaa atggcgtttc ggtattcgct
       61 ccaacgcgaa aacgatattg accggggaag cctgcgcgcg ccaattggta cggctgacaa
      121 ccgacaacat tcatttaaca actatcagtc gtttgtctat aactgggtgc ataccttttc
      181 gcccagcctg ctcaatgact ttaccttcca cgaaaacaat tttatcaacc ggattccaac
      241 ttttgttgaa aaccgcaacg aactccggtt tccttcagtc caggatggcg ggaatttccg
      301 catcccacag cgcacgcgtc aaaaccggat tcaatttcgg gacaatctga gctggatttt
      361 gggtcagcat acagtcaaat tcggtggcga atatcatcgc ctcgacaccg atgcgatttt
      421 tgatttgttt gggtcgggca cgatttttct ggccgaagat tttgcctcac aggatcgcaa
      481 ccgtgacggt tcactcaatg acctggatat tccacttgtg ctggcgctcc gatccattgc
      541 cccaaatcgt ccaccaaccg ttcccaacgt agacaacaac tttgtggcct tctatttcca
      601 ggatgactgg aaagtccgcc ccaatctgac catcaatctc gggttgcgct atgaactcga
      661 caccgatgtc aaaaacatca gtaactttga ccagatcaat ccgctggtgc tgccgttttt
      721 ggcaccgggt gggcgctcgc gaaataccaa aaactttgcc ccgcgcgttg ggttcaactg
      781 ggatccgtgg agcgacggca aaacctcgat tcacggtgga tatggcattt actatgaccg
      841 ggtcatcctc gaagtgcaat tgctcgaacg attgctggat ggtcggacac ttccacttga
      901 agcccgcacc ggatctgccc ttgatgcctc aggaaacttt gtggcaggca caccgacact
      961 tgcggcgccc tttaccggac agattatccc tggcgctggc gccgtcggca tcaacatcat
     1021 tgataacaac cttgatacgc catatgtcca gcagttcaac tttggctttc aacgtgaaat
     1081 catgaatggt ttgaccgtga gtgtagacgg ggtacattca tttggatcga aattcatcat
     1141 cgggcgtgcc attggtgcgg tgtttaaccc ggtggttggc ggtccggatg gcatcgtcaa
     1201 tatcgaatcc tcggtcaaaa actggtatga cgggctgctc atcagcgtgc aaaagcgaca
     1261 ttccaaccac ttcagtttta atgcgtcgta cacgctttcg aagaccttta ccttctcaaa
     1321 tgacgaccag attccatttc aggccggacc gcttgatcca aacaacctga acctcgaaaa
     1381 aggcccggcg cccaatgatg aacgccatcg gttcaccctg gcggcactgt ttgatctgcc
     1441 gctcggtttt cagctttcgc caatttacac agtgacctcg ggggtaccgt ttgacattgt
     1501 attgcccaat ggcacaacgc ggattcccta catccagcgc aacgccgccg ggcgacaatt
     1561 caagaccgga gcggatctca atgaattcat cacccaaatc aacgccggag gtggaattgc
     1621 cggggttggg ttgctcccac tcgtcaatga caacctgaga tttggcgaca gtttccagtc
     1681 gtttgacctg cgactcacga aaacctttac catcggcgac cggctcaaag tgcagggcat
     1741 tgccgaagtg ttcaacctgt ttaatgtgac caacattcgc ggtgtgacaa atgtgaatta
     1801 ttccggtttc caaaacacgt tgattcgcga cagtcaggat ccaaataacc ctgggttttt
     1861 gcgatcatca cggtttggaa caccaattca aacagcaggc ggcgtgtttg gaacgggcgg
     1921 cccacgcgcc tttcagcttg cagttcgggt caatttttag agcgaaattc tcaagaccca
     1981 gtaccccaaa ctacatttgg cccaaatccc tgaagaggag ttaccttgcg gtgctcctct
     2041 ttttttgttt gaacaaaccg ctattcaccg tgaatgaatg attatcggct acggtcattt
     2101 ttagatttgc ttccgaaact tcaccagata gcgttgatcg cccgaaggta atttcacggt
     2161 ttcgacggtc cagccacggt gggggttctg actcaaggtt tgagccgtgt ctccgccaac
     2221 ccacaaaaaa gcgacctgga gcccaggcag tttcaaaagt ttcggcaatt gtttgtccat
     2281 tttttcaatc gcccgcgcga ggattcccca gcgcgggatt ttttctgtcc cgaagagcgc
     2341 cgccagttga ccggcttcaa cacggccttc aaaaaccgtc acgtgtgatt caagccccag
     2401 attttggatg acttcattga ggaactgggc tttcttatgc tggctatcgg tcaataaaaa
     2461 atgttccacg tggaacatga ttgacagtgg cacacctggg aaaccaccgc ccgaaccaac
     2521 atcaacccag acctccactt cagtgttgag tgcttccaga tatggtgcgg cagccagtga
     2581 gtccagatag tgcaaccggg cggcggcctc cgggttggta atcgtcgtca aactcatgcg
     2641 ctgattccag tcacagagca catcaaaatg cccggccaac tgctcaagct gcagttcaga
     2701 aaagtgaagc ttgttttggg aaagcaatgg acgcaggtaa gtcagaaggg aagacatagg
     2761 agtggggttg aagactatcg ggctgaagaa atcgggctga agaaatcggg ctcagggtat
     2821 tgaacccgaa gtcttcagcc ccgagtttac gagtcttcag ccccaggttt tcagcccgct
     2881 gtcttctacc cgagggtttt catccccggc ggtgaatttc gaggtgcatg ctcaaaatgg
     2941 caattgccgc cggcgtaacg ccaggaatgc gtcccgcctg gccaattgtt tccgggcgga
     3001 ctcgtgaaaa tttctcgctc atttcgcgcg aaagacctgg aattccacgg aaaacaaaat
     3061 ccgttgggat tcggacttca gccgctttcg ccattttctg ggcggtgctt tcctggtgct
     3121 tgacgtagcc ggcatatttc aaatcattga ccgccacgcc aagctcttcc tcaataaagt
     3181 tttcatggcc cagttgacgc agcactccgg caatggcttc gatggtaact tccgggcggc
     3241 gagccagtgt cgccagtcgg gtcgtttctc gaatctgtgt gcctccggtt gtctgacgga
     3301 aggtctcata cacctcagac gttggcgaga ggggcgtctt ttcaagcaag gtatgcaatg
     3361 cgttgacacg ttcctgtcgg gctaaaaatg cctgatactg ttcgtcattg accataccaa
     3421 cctgccgtcc aagtggtgtc aggcgctggt cggtattgtc aattcgcagc aggagtcgga
     3481 cttcagaacg cgaagtgaac atccgatacg gctcatccac gccactggta atcaggtcat
     3541 caattaagac tccgacataa cttttggcac gatcaagcgt gacttgttcg cggccaagcg
     3601 ccatcaggcc agcattcata ccggcaatca acccctggca gcccgcttct tcatagccag
     3661 tcgtcccgtt gatttgcccg gcatgaaaca ggccgcgcac ccgtttgact tcgagcgtcg
     3721 gacgcaattc ggtcggatcc acaaaatcat actcaatcgc atatccaggc cggatcaact
     3781 gtgccttttc aagaccggga attgagcgca cgattttttc ctgaacgtca atcggaagac
     3841 tggttgaaag gccgttgaca tagacttcgt tggtgtgatg gccttcgggc tcaaggaaaa
     3901 tctgatgccg gtctttgtcg gcaaatttga cgatcttgtc ttcaatcgaa ggacaatagc
     3961 gcggtccggt ggattggatt tgcccgctgt agagcggcga cttatccagg ttttcccgaa
     4021 tgatgcgatg cgtgtctggc gtcgtgtacg tgatgtaaca cgacaattgt ggctgcacga
     4081 ctttttgcgt ctggaacgag aacggaaccg gggtggagtc acccggctgt tcttcgagat
     4141 tcgagaaatc aatcgttcgg gaattgatgc ggggtggcgt tccggttttc agccgcccaa
     4201 cccgcagccc cagcttgcga aacgaagccg ccagcaaaat cgaaggcaat tcgccagacc
     4261 gtccagccga ataggtgcgc ggtccgatat gaattaagcc attgagaaac gtgccggtac
     4321 aaacaaccag cgctttggtg gcaatcttac ggccatccca catctcgaca ccgatgatgc
     4381 gatcctgctc gaccatgatg tcaatcactt cgccctgtcg cagatgcaaa ttgggaacag
     4441 tttccaggta gcggcgcatt tccgtacgat agagaccccg gtcagcctgc gcacgcggcg
     4501 actggaccgc tggcccacgg ctgcgattaa gcaaccggaa ttgaatcccg gttcgatcaa
     4561 taatctggcc catcaggccg ccaagagcat ccatttcgcg cacgagatgg cctttggcaa
     4621 tgccaccaat cgccggattg cacgacatct gggcaattaa atcaaggttc aacgtaatca
     4681 gcgccgtggt gcagccaagt tgagccgcag cgtgtgccgc ttcacaaccg gcgtgtccgg
     4741 ccccaatcac aaccacatca aatgcttcgt taaaacaata ttcaactgac atattcacca
     4801 catcaatact gaaaaatgag gtctctcgcg tgctcctcaa atcatgctga tacaagatgt
     4861 ttagggattt tgaaggaagg gaagcagaat acaggattga acgcccggtg cgaagcctct
     4921 ttgccgaatg cctctttgag ttggcttcgc agtttcaatt catcagaggg tgtggcaagc
     4981 taacgctgag ggctgagggc ctggggctga agattcgcaa gctcagggct gaagaaaacg
     5041 ggctgaagtt aaggaccagg aggaaacaat gaatacacag gaattaagtg ccagagttgt
     5101 tcaacaactc ccagttgaaa cacaagaatt actttttcct ctcatctcag aactacgggc
     5161 gcttccaatt gaaacacaac ttgacttctt agcagatttg cacaaagagc tatctcaacg
     5221 aaaagttcaa tcacaatctg tccttgaagc aatcatttcg ccttcattac cagatgaaga
     5281 tcaggaagtt cgggcaacaa agcgccaaca acgagaggaa tggttgaaag cgaattatgc
     5341 tgactatgct ggaaaatata tcgctcttga tggaaatcgg ttgctaggag ttggtcaaaa
     5401 ttatgcccag gtccgaaaag cagcccttca agcaggcgta aaggatgcct acattgactt
     5461 tgtgcatccg cctaatgcca aaggttttat gggagggtgg taatggctta tcacttaatc
     5521 ttcaaccaat tttccagtta tgatgccggg caacctggaa tcaccatacc tgtt
//
LOCUS       DAOQDO010000076         5538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9172_length_5538_cov_2.861025, whole genome shotgun sequence.
ACCESSION   DAOQDO010000076 DAOQDO010000000
VERSION     DAOQDO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9172_length_5538_cov_2.861025"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1118)
                     /locus_tag="PKZ53_02060"
     CDS             complement(144..1118)
                     /locus_tag="PKZ53_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ39245.1"
                     /translation="MYTKALHKRIVLLSLMFWVVVLLGGCQKSIPPSGQTGIVITDAE
                     GKSIPISDTSRLVTVGTALTETVLALGAGSRLVGVDDASTNYLSEVSQLPKVGSFRTV
                     SAEGILSLNPSLIIATADAGPPEAISQLKTAGAPLLILPVNYTVEGVKTQILTVARAL
                     NKVDKGNELTQAIDQDMASVQSLLAKPHPRLKVMFCGRGPNMPTATISGKGTGIDEMI
                     TLAGGDNPFTSFQGFRPMTDEAVIAAAPDVLLMTERSFERVGNLDGAVQLPGIPLTPA
                     GKNRRVIPVSDRYFQGFGPGIGTAVRDLALKFYPELQAQPGKVVPKKS"
     gene            complement(1193..4072)
                     /locus_tag="PKZ53_02065"
     CDS             complement(1193..4072)
                     /locus_tag="PKZ53_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39246.1"
                     /translation="MSKLKPRSERYAIPPIWQLVACLGWVLASVFISSVAYGQALNTA
                     TIRGQVLDQSRAAITNAQVVVTNNQTGVHRETTTDEKGNFTCASLPLTGEYTVLVSQS
                     GFAAEEVTDVTLQAGEAASFEITLKPQGENSVVTVSGTTEGVRADSPQLSTRLDLQKI
                     DNTPILGRKVTSLVLLDSAIRPARGTGDLFLNNTLFVANGSGRRQTGFIVDGSTGDDA
                     WGRQSIFTNIPFSAIQEFTILTNGFSAEFGRTTGGVVNLVTQSGTNTFHGDLLGLFRP
                     GGIQARQPLATRQTEDELAQVSGVFSGPIIENRTHFLVSAEYSHQDRDAVITSPLAPG
                     LFTGTYKQGLFLARLDHQLNNRNALEFKLNFDRFSDTNPSDAVGGVALPSSARIFRGR
                     AYEAQLSETAILSDRIVNEARAQFQLGSPITQFEPVMPSTQYIRPGISTEGESRSAKL
                     QNRQFQFADTLSIVAGKHIVKVGGDVVYTSSGGFGQEFGGGFVLGQITLNPGVLTPIG
                     QLQAKDVQRFSQSFGNADYTVRQTLSSVFIQDDIKLRSDLTLNLGLRYENQSLTDDHN
                     NIAPRIGFAYNIGGDTKTVLRGSYGIYYSQLRANIVAGFKISGPTGVFTFSAAPGQLG
                     FPENLLPLSGFPAGAVLPPRDIQVRPGDGEFLSQFFDVSKLRGYPDALLNPYTQLWSL
                     GVERELGQKWFLRVDYVGQHTIGIDRPVDLNSPAAFVRTKPGQVRSAAEADATRPIKP
                     VPNGYRRILATINEGASNYHALQINLNKRFSQNFSVLASYTYSNSINTVEPDAPGQDP
                     TDATMLGKPERARSVLNQTNRAVFSGWYQLPLRFTVGGIASLASGRPYNITTGLDNNG
                     DGSTADRPVVNGFILGRNAGKGTPVYDVGMFVEHTFKLPAERVKLSLRAEAFNLFNHN
                     NIVGRNGVFGNDPTGKPLATLGKPLGGISNVDPGRQFQFVARVRF"
     gene            complement(4477..>5538)
                     /locus_tag="PKZ53_02070"
     CDS             complement(4477..>5538)
                     /locus_tag="PKZ53_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNJ39247.1"
                     /translation="IGVLTIIVILVWNAVAKKIKMIPAPLVAVVSAVVVSGLMNLDIK
                     YVQIPGSFLDILNVPSMESVKSVTHLPIFLASISVAFIASAETLLSAAAVDKMHSGVR
                     TNYDRELFAQGIGNALCGMLGALPMTGVIVRSAANVEAGAKTRLSTILHGAWLLVFVL
                     CLPWMLEKIPTASLAAILVFTGYKLVNPSKMKEILAYGRSEFAIYLGTMVAIVATNLL
                     EGVLIGLGMSIAKLIYVFTNLDSRLEITDGGKQARLTMRGSATFVMLPKLAALLDQVP
                     AGAELQVHFESLDYIDHACLDLLMNWEKQHELTGGEMCIEWDELEAKFHRRRPDWSMT
                     PDKLASSDEKVQNDLAVKK"
BASE COUNT         1411 a         1491 c         1355 g         1281 t
ORIGIN      
        1 cattggtgag agatacaccg cccctttgcc agttgagaag ataacaatca ccaccaatcc
       61 aaccacaagc cctgccagaa acaaatggac ccgcgatggt gacgcttttt cagccagttt
      121 tgaaaaccgc cgtcccagca gcgtcatgat tttttgggaa ccacctttcc cggctgagct
      181 tgaagttccg ggtaaaactt caacgccaga tcacggactg ccgtcccaat ccctgggcca
      241 aatccctgga aataccggtc actgacagga atcacccgcc gattttttcc agccggagtt
      301 aatggaatgc ccggcaattg aaccgctcca tccagattgc caacgcgttc aaaactgcgt
      361 tcggtcatca acaacacgtc aggtgcagca gcgatgaccg cttcatccgt catgggccga
      421 aacccttgaa agctggtaaa tgggttatct ccaccagcca gggtaatcat ttcatcaatc
      481 cctgttccct ttcctgaaat cgtcgccgtc ggcatatttg gcccacgacc acaaaacatg
      541 actttgagtc gaggatgcgg tttggccagc agcgactgga ccgaagccat atcctggtca
      601 atggcctgag tcagttcatt ccccttgtca actttgttca atgccctggc aacggtcaga
      661 atctgggttt ttacaccttc aaccgtgtaa ttgaccggca aaatcaacag tggcgcgcca
      721 gccgttttga gctgactgat ggcttcaggt ggtccggcat ccgccgtggc gatgatgagc
      781 gaggggttga gcgacaaaat gccctcagcc gaaacagttc ggaacgatcc aactttggga
      841 agctggctca cttcagataa ataattggtg ctggcatcat ccactccgac cagtcgcgac
      901 ccggctccga gcgccagaac ggtctctgtc agagcggtac caaccgtgac cagtcgtgaa
      961 gtatcgctga tcggaatgga cttgccttca gcatcggtga tgacgattcc agtttggcct
     1021 gaaggaggga tagatttttg gcatccgccc agcagaacca ctacccagaa cattaaactc
     1081 agtaaaacga tccgtttgtg cagtgctttg gtgtacattc agtgcctcgt ggcaccgggc
     1141 aggcacctgg tgactgcccg gtagcaaaca gttcaaaccc gaaatctggc cactaaaacc
     1201 gaaccctggc cacaaactga aattgacgac ctggatcaac attgctgatc ccgcccagcg
     1261 gcttgccaag cgttgcgagc ggcttcccag tcgggtcatt gccaaacacc ccattgcgac
     1321 ccacgatgtt attgtggtta aacaggttaa acgcttcagc ccgcagactc aatttcaccc
     1381 gttcggctgg aagcttgaat gtatgctcga caaacatccc gacgtcatag accggagtgc
     1441 ctttacccgc atttcgtccc aaaataaacc cattgaccac gggccggtca gccgttgaac
     1501 catcaccatt gttatcaagc ccggtggtga tgttgtatgg tcggcctgac gccagcgagg
     1561 cgataccgcc aactgtaaat cgaagcggca attgatacca tccgctaaac actgcccggt
     1621 tggtctggtt gagcacgctc cgggcgcgtt ccggtttgcc gagcatggtg gcatcggttg
     1681 gatcctgccc aggagcatca ggctccaccg tgttgattga gttggaatag gtataactgg
     1741 ccaggacaga gaaattctgg ctgaagcgtt tgttgaggtt gatctgaagc gcatgatagt
     1801 tgctggctcc ctcgttgatg gtcgccagaa tacgccgata gccgtttgga accggcttga
     1861 tgggccgcgt ggcatcggct tcagcggctg accgaacctg acccggtttg gttcgaacaa
     1921 aagcggctgg tgaattcaga tcaactggcc ggtcaatgcc gatggtgtgt tgtccgacat
     1981 aatccacccg caaaaaccat ttctgaccga gttcgcgttc aacacccaga ctccataatt
     2041 gagtgtatgg attgagcaag gcatctggat agccacgcaa tttggaaaca tcaaaaaact
     2101 ggctcaagaa ttcgccgtca cctggtcgaa cctggatgtc acgcggcggc aacacggcac
     2161 cagcgggaaa accactcaat ggcaacaaat tctcaggaaa tccgagctgg cctggagcag
     2221 cactaaaggt aaacaccccg gttggtccgc tgattttgaa tccggcgacg atattggccc
     2281 gcagttgtga gtaataaatg ccataactgc cgcgcaatac agttttggta tcaccgccga
     2341 tgttgtaggc aaacccaatt ctcggggcaa tattgttatg atcatcggtc agggattgat
     2401 tttcatagcg caggccaagg tttaacgtca ggtcagatcg aagtttgatg tcatcctgga
     2461 taaaaactga cgacagggtc tggcgcacgg tgtagtcagc attgccaaag ctctgcgaaa
     2521 accgctgtac atctttggct tgtaactgac caatcggggt taaaacccct ggattcaacg
     2581 taatttgccc caacacaaac ccaccaccga attcctgacc aaagccacct gacgaggtat
     2641 acaccacatc tccgccaact tttacaatgt gctttccagc gacaattgac aatgtatcgg
     2701 caaattgaaa ctggcgattt tgcaacttgg ccgaacgtga ctcaccttcg gttgaaatac
     2761 cagggcgaat atactgggtt gacggcatta ctggttcaaa ctgggtgatt ggcgacccga
     2821 gctgaaattg agcacgagct tcattgacaa ttcgatccga caaaatggca gtttccgaaa
     2881 gttgtgcctc atacgcccgc ccgcgaaaga tgcgggccga actcggaagg gccacaccac
     2941 ccaccgcatc gctcgggttg gtatctgaaa accggtcgaa atttaatttg aattcaagcg
     3001 cattccgatt attgagttga tgatccagac gcgccagaaa cagcccttgc ttgtaggttc
     3061 ccgtaaacaa ccctggcgcc agtggagagg taatcaccgc atcacggtcc tgatggctgt
     3121 attccgccga aaccaggaaa tgggttcggt tctcaatgat cgggcctgaa aacacaccac
     3181 tcacctgggc cagttcatct tctgtttggc gtgtggccag cggctggcga gcctgaatac
     3241 ctccaggtcg aaacaacccg agcagatcac catgaaacgt atttgtgcca gattgggtca
     3301 ccaggttgac cacgccaccc gtggttcttc caaattcagc cgagaaccca ttggtcagaa
     3361 tggtaaattc ctggatggct gaaaagggaa tattggtaaa gattgattgc cgcccccagg
     3421 catcgtcacc cgtgctgcca tccacaatga agccggtttg acggcggcca cttccattag
     3481 cgacaaacaa ggtgttgttt aaaaacaggt cgccagtacc acgggctgga cggatggcag
     3541 aatcaagcaa caccagcgac gtcacttttc ggcccaggat cggggtgttg tcgatttttt
     3601 gtaagtcgag ccgggttgaa agctgtggtg aatcagcacg gaccccctca gttgtcccag
     3661 atacggttac cacgctattt tcaccctgcg gtttcaaggt gatttcaaag ctggcggctt
     3721 caccagcctg aagggtaacg tcagtgactt cctcagcagc aaacccggat tgcgacacca
     3781 aaaccgtgta ctctccggtt aacggaagac tggcacaggt aaaatttcct ttttcatcgg
     3841 ttgtcgtttc acggtgaact ccagtctggt tgttggtcac aaccacctgg gcatttgtga
     3901 ttgccgcccg gctctggtcc agtacctgcc cccggatggt cgcagtattt aatgcctgac
     3961 cataggcaac cgatgaaata aatacactgg ccagcaccca gcccaggcag gcaacaagtt
     4021 gccagatggg aggaatggca tacctctcgg atcgtggttt gagtttcgac atgaatttgt
     4081 cagtccttct gttttgggat tttcgggaac ggaagtgagt gggttgtgtg gagcaccctg
     4141 ctggaagaca cataccagca gatatgtgag gctcttacag gtttaaggaa cccaatctat
     4201 atggactgat tttttcgttt ttttcttcca taaataacta gaaaattcac actctacatc
     4261 aggaattgca gtatgtattt cattaaaaat aaacatttta accacaaaaa tataattttt
     4321 cttttttgta tcaaaccact ggcatcatca ccctggaatg aatctgagtg tattggttat
     4381 gcaaatcatc agcgaggaac cacaaaaaaa cccgcctgcc cttgatcagg acatggcggg
     4441 tttggcacct cataccaggc acgctttcaa ctcaactcat tttttgacgg ccagatcatt
     4501 ctgaaccttc tcgtcagagc tggctaactt atccggagtc attgaccagt caggtcggcg
     4561 gcgatggaac ttggcttcaa gttcatccca ttcgatacac atttcaccac cggtcagctc
     4621 gtgttgcttt tcccaattca tcaacaagtc cagacaggca tggtcaatgt aatccagcga
     4681 ctcaaaatga acctgcaact cagccccggc tggcacctga tcaagcaatg ccgccagttt
     4741 tggcaacatc acaaaggtgg cgctgccacg catggttaat cgggcctgtt ttcccccgtc
     4801 agtgatttca aggcgcgaat ccagattggt aaagacataa atcagcttgg caattgacat
     4861 tcccagccca atcaggaccc cttccaacaa attggtggcc acaatggcaa ccatggtgcc
     4921 gagataaatg gcgaactcac tgcggccata ggccaggatc tctttcatct ttgaaggatt
     4981 gaccagctta tagccagtaa acaccagaat cgcggccaga ctggcagttg ggattttctc
     5041 aagcatccag ggcaggcaca acacaaacac caggagccag gcgccgtgca aaatcgttga
     5101 cagtcgggtt ttagccccgg cttcaacgtt ggccgcactc cggacaatca caccggtcat
     5161 cggcaaggcg cccagcatcc cgcacaaggc atttccaatt ccctgggcaa acagttcccg
     5221 atcatagttg gttctcacac ctgagtgcat tttatcaacg gcggcggcgc ttaagagcgt
     5281 ctcagcactg gcaataaagg caacggaaat gctggccagg aaaataggaa gatgagtaac
     5341 gcttttgacc gattccatgg agggcacgtt gagaatatcg agaaagcttc ctggaatttg
     5401 cacatatttg atatccaggt tcatcaatcc agagacaacc accgcactga ccacggccac
     5461 gagtggcgct ggaatcattt tgattttttt tgcaaccgcg ttccacacca gaatgacaat
     5521 aatggtcaac accccaat
//
LOCUS       DAOQDO010000077         5532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9190_length_5532_cov_3.215081, whole genome shotgun sequence.
ACCESSION   DAOQDO010000077 DAOQDO010000000
VERSION     DAOQDO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9190_length_5532_cov_3.215081"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKZ53_02075"
     CDS             <1..868
                     /locus_tag="PKZ53_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNJ39248.1"
                     /translation="AEYLPFSGEITLRGATPQSLPVILERAVAELLIKTQEGTRVYVN
                     GQETGIVPATGELRVSTLLPDQPNTVRLERFGYEKFEQLIRGQAGKTVELETRLLPLP
                     TTTEFADNFQGGLQFWEAPSEWKAETGILTIPTTSRIGVVKEKRFCESEVFFGLRLTN
                     DRGAAWVVRAQDEKNYYLFCLNGPGGIFPNKFQTYVCRDGRLDLTKPATAPLPILPPL
                     KPGETYRVHIKISGTTIQHWITPSKTGEEISIGFFQDAQNTFPCGTLGFSTPAGEAFQ
                     VTGFVVSPLSAN"
     gene            complement(961..4419)
                     /locus_tag="PKZ53_02080"
     CDS             complement(961..4419)
                     /locus_tag="PKZ53_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ39249.1"
                     /translation="MKKPILVLAAWFVWCGSLLLFVGDVTAQTVTGTIAGQVIDQQQI
                     PLAGVLVRFINLENGFDYGKRTDRLGTYRIEFLPAGAYQIVAEKDGFRPTVIAQFLVE
                     VNRQKIIKPPPIQLIPLNSSPPATSPDQDKSRLAQVNVTDAALRGSFPAGFLNALPLA
                     GIRNFDRLAFLFPGIAPPPATTGANGPGIGPGVGTAGQFSVNGQRARSNNFTVNGSDN
                     NDQEVGVRRQGFTPAIPIPVEGITEFQVTTLLADSEAGRNTGGQINVLSRSGSSAIHG
                     EFHDFLTGSALKARDFFDLSGPGNPAKNAAIQNQIGASLGFPIVANRWHILTAFEHQL
                     SVRDQESHFAVPTLTERNTALQSARQRSRLGVDVLDPTFYPLPNDPGGPYGGNTLTKI
                     LSADGNGLVVNLGLDGQTAVWGRPTTVSLRAHFTDDDTRIPVVDDAINSSVTALTRTH
                     NLALVASTELSSRLANQFRFSFGRTALRFAEVADSPFIFQSRLTNRDRTGDGIPDGRT
                     GPIGRLLLSPYSPLGVDPGTFPQRRVNNTFQVAETLVREFSRVTLKAGADVRRVQINS
                     ALDRNYRAQVAFTPGFLVTRDGLQTGSGADFAAFGLPSDIFQALAVTPDSSLAVRFTE
                     LNLFTSAQVRLSPNFTVELGLRYERNTVPTDATGRLERSLTVQNSELPPFDPESAFAQ
                     IFLGALDAQRSFLGGRRNIIRGDGDNVAVRAGFAWDLSKTGRMSLRGGYGLFYDPILG
                     TVVSQSRNALPSFIPVNFGSSVLFPNALSANPAFLRFGAQLDQSLIVPGTVNTISFAP
                     EDYVSGIGRLLTIGGFFVGSGFGAAITLPDQNLASPTVHQYSLSLERTLPNGFTASLA
                     YVGTTGRNLLRVRTPNGGQLTPLAIRVLAGGIPAVIRLQNRPNPDLGAVTVFESSAHS
                     TYNGLQASLVRRFTTGLAVQVSYTWSHAIDDVSDIFDTSGASALAQDETGRVSSLKTE
                     RGNAAFDVRHRLTSAWSVPISLKKLKLGDFQLSGIVTLQTGQPFTVTSGFDTNFDGNL
                     TDRINTTNGLLISNRGRTRLQILPGVDVFQFLPPIDSDHPSNGQVGRNTFRAAGIASL
                     DVALTKKISLRKEHSLELRVEAFNLLNRTHFGIPVRVLNAPGFGSSVNTSLPARTIQA
                     AVKLRF"
     gene            complement(4434..>5532)
                     /locus_tag="PKZ53_02085"
     CDS             complement(4434..>5532)
                     /locus_tag="PKZ53_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39250.1"
                     /translation="NLFICDSFNNRLRRVDAQTDIITTVAGNGIAIERREGDLATSYF
                     LQIPTDVAVTSTADILVSDLVTRQVFRVDAHTGRVALFAGNGKIESKGDGGPATKASF
                     DTVSQLGVDEKDNLYIVDSASLRVRKVTAQTGLITTIAGNGKSGASGDGGPATAASLE
                     PWAVLPDLINNQLLIADPNNSQIRRVDLRTGLISTLVSARQVGSELIKGKRFSPGSLA
                     IDREGNLFISDPPNNRIRRLDARTGMISTVVGGNEFGYRGDNGPASEALINQPGPLVI
                     DGAGNLFFADLSNRVIRKIEAQTGVISTVAGNGPLDQFGSDGDGKPAINVWLASISGL
                     AVDAAGNLYLAETRATGESSAVRVIKGIAIP"
BASE COUNT         1362 a         1488 c         1478 g         1204 t
ORIGIN      
        1 ggctgaatat cttcccttct ccggcgaaat cacactccgt ggcgcaacgc cacagagtct
       61 tcccgtgatt ctggagcggg cagtggcaga actcctgatc aaaacccagg agggcacacg
      121 ggtttatgtc aatggtcagg aaactgggat tgtcccggca accggagagc tacgggtttc
      181 gacgcttctt cctgatcaac caaacaccgt gcggctggaa cgttttggat atgaaaaatt
      241 cgagcaactg attcgtgggc aggctggaaa aacggtggaa ctcgaaacac gattgcttcc
      301 cctgccgacc acaactgaat ttgccgacaa ttttcaaggc ggccttcagt tttgggaggc
      361 gccgtcggaa tggaaagctg aaaccggaat tctcaccatc ccgaccactt cgcggattgg
      421 cgtggtgaaa gaaaaacggt tttgtgaaag cgaagtcttt tttggattgc gcctgaccaa
      481 tgaccgtggg gcagcctggg tggtgcgggc ccaggatgaa aagaattact atctgttttg
      541 tcttaacggc ccaggcggca tatttcccaa caagttccag acctatgtgt gccgcgatgg
      601 aaggctggac ctgaccaaac cagcaacggc gccacttccg atcctgccac cgctcaaacc
      661 cggcgaaacg taccgggtac acatcaaaat cagcggcacc acgatccaac actggatcac
      721 cccgagcaag accggcgaag aaatttcaat tggttttttt caagatgccc aaaatacctt
      781 tccctgcggc acacttgggt tttcgacccc agccggtgaa gcctttcagg tcacaggttt
      841 tgtcgtgtca ccgctttcag ccaattaggg ttccgggttc cgggtcttcg aatttatgtc
      901 cttttcgtcc tttttgtccc ttttgtccct tttgtctttt tcctggaacc ctcattggca
      961 ttagaacctc aatttcacag cagcctgaat cgttcgtgca ggaagtgatg tgttgaccga
     1021 agacccaaat ccaggggcgt tcaagacgcg cacgggaatc ccgaagtgag tgcggttcaa
     1081 caggttaaag gcttcgacac gaagctcaag cgaatgttct ttgcgaagag aaattttttt
     1141 ggtgagagcc acatcaagcg aggcaattcc agccgcccga aaagtgttcc ggccaacctg
     1201 accattgctc ggatggtcgc tgtcaatcgg cggcagaaat tgaaaaacat caacgcctgg
     1261 aagaatttga aggcgagtcc ggccacgatt tgaaatcagc aatccattcg tggtgttgat
     1321 tcgatctgtc aagtttccat caaaattggt atcaaacccc gaagtcaccg taaatggctg
     1381 gccggtttgc aacgtcacga tccctgaaag ttgaaaatca cccaacttca attttttcaa
     1441 tgatatcggt acactccagg ccgaggtcaa ccgatggcgg acatcaaaag cggcgtttcc
     1501 acgttcggtt ttcagcgatg acactcgccc ggtttcgtcc tgagccagtg ccgaggcacc
     1561 tgatgtgtca aagatatctg aaacatcgtc aatggcgtgt gaccaggtgt aactcacctg
     1621 cactgccagc ccggtggtaa accggcgtac cagcgaggct tgcaacccgt tgtaggtgga
     1681 atgcgccgaa ctttcaaata cggtcacggc ccccaggtcg ggattgggcc ggttttgcaa
     1741 ccgaatgacg gcaggaatac caccggccag cacccgaatg gccagcggtg tcaactgacc
     1801 accgttgggc gttcgcacgc gcagaaggtt tcttccggtt gttccgacat aggccagact
     1861 ggcggtaaac ccattgggca gcgtccgttc gagcgagagt gaatactggt gcacggtggg
     1921 cgacgctaag ttctgatccg gcaaggtaat cgccgctcca aatcctgacc cgacaaaaaa
     1981 accgccaatc gtaagtagcc ggccaatgcc ggaaacatag tcttcaggtg caaacgaaat
     2041 cgtgttgacg gtgccgggaa caatcaacga ctggtcaagt tgcgctccaa atctgagaaa
     2101 ggccggattg gcactcagcg cattgggaaa caaaaccgaa gaaccgaaat tcacgggaat
     2161 gaaactgggc aaggcgttgc gcgattggct gaccacggta cccaaaatcg ggtcataaaa
     2221 caaaccgtac ccgccccgaa gcgacatcct tcccgttttt gaaagatccc aggcaaaccc
     2281 agcccgaacg gcaacattgt cgccatcacc ccgaatgatg ttgcgtcttc cgcccagaaa
     2341 actccgctgg gcatcgagcg cccccagaaa aatctgcgca aaagctgatt cagggtcaaa
     2401 aggtggaagt tcggagtttt gaacggtgag tgagcgctcg agtcgcccgg tggcatcggt
     2461 cggaacggtg ttgcgttcgt aacgaagccc taactccacg gtaaaattgg gggaaagccg
     2521 cacttgagcc gaagtaaaca ggttgagttc ggtaaaccgt accgccagtg atgaatcagg
     2581 tgtcacggcc agcgcttgaa agatatctga cggcaaccca aaggccgcaa aatcggcacc
     2641 agagcccgtt tgtaagccat cacgcgtgac cagaaatcca ggtgtgaagg cgacctgggc
     2701 ccggtagttt cgatccagtg cactgttgat ttgaacccgg cgcacatcgg cgccggcctt
     2761 gagtgtgacc cggctgaatt ctctgaccag cgtctcagcc acctgaaacg tgttattcac
     2821 ccggcgttga ggaaacgttc cagggtcaac gccaagcgga ctatacggcg acaacagaag
     2881 tcgcccaatc ggaccggttc gtccatcggg gattccgtct ccggttcggt cgcggttggt
     2941 cagacgagac tggaaaataa acgggctgtc agcgacttcg gcaaagcgca aagccgttcg
     3001 accaaatgaa aagcggaact gattggccag ccgggaagac agttcggtac tggcgaccag
     3061 tgccagattg tgggtccggg tcagcgccgt cacactcgaa ttgatggcat cgtccacgac
     3121 tggaatccgg gtgtcatcat cagtgaaatg agcgcgaagg ctgaccgtgg tcggccttcc
     3181 ccaaacggct gtctgtccgt ccagccccaa attgacgacc agcccatttc catctgcgct
     3241 cagaattttc gtcaatgtgt tgccgccata gggtccgccg ggatcgtttg gcagcgggta
     3301 aaatgttgga tccaagacat caactccaag tcggcttcgt tgtctggctg attgcagcgc
     3361 ggtgttgcgc tcagtcaacg taggcacagc aaagtgactt tcctggtcac gaacactcaa
     3421 ctggtgctca aacgcggtca agatatgcca ccgattggcc acaatcggaa acccaaggct
     3481 ggcaccaatt tgattttgaa tcgctgcgtt tttggcagga ttgccgggac cgctcaggtc
     3541 aaaaaaatca cgcgctttca gggctgagcc agtgagaaaa tcgtgaaact caccgtgaat
     3601 ggcgcttgaa ccagaccgtg agagcacatt gatttgacca ccggtgttgc gcccggcttc
     3661 ggaatctgcg agcagcgttg tgacctgaaa ctcggtaatg ccttcgactg gaatcggaat
     3721 cgccggagta aacccctgac ggcggacacc aacttcctga tcgttgttgt ctgaaccgtt
     3781 gaccgtgaaa ttgttgcttc gggcgcgttg cccattgact gagaactgac ctgccgttcc
     3841 gacgccagga ccgatccctg ggccattggc gccagtggtc gctggcggcg gggcaattcc
     3901 aggaaacaaa aatgccagcc gatcaaaatt cctgatgccc gccagtggca gtgcattcaa
     3961 aaaaccggct ggaaaacttc ctcgcaatgc cgcatcagtc acgttcacct gagccagccg
     4021 ggatttatcc tggtcaggtg aggtggcagg cggcgaagaa ttgagtggaa tcagttggat
     4081 gggtggtggc ttgatgattt tttggcgatt gacttcgacc agaaactggg caatgacggt
     4141 ggggcgaaaa ccgtcttttt cggccacaat ctgataggct cctgccggaa gaaactcgat
     4201 gcgataggtt cccagccggt cagttcgctt accgtagtca aacccgtttt caagattgat
     4261 aaaccgcacc aggacaccag ccagcggaat ttgctgctgg tcaatgacct gtccggcaat
     4321 ggttccagtc acagtttgag cagtgacgtc tccaacaaac agtaacaagc tgccacacca
     4381 gacaaaccag gctgccagaa ccagtattgg tttcttcatt ctcaattctt cattcaagga
     4441 atggcaatgc ctttgatgac tcggacggcg ctgctttcgc cggtcgcacg ggtttcggcg
     4501 agataaaggt taccagccgc atccacagcc aatccggaaa tagaagcaag ccagacattg
     4561 atggcgggct ttccgtcacc atctgacccg aattgatcaa gcggaccgtt accggctacg
     4621 gttgaaatca ctccggtttg ggcttcgatt ttgcgaatga cccggttgga taaatccgca
     4681 aaaaagagat tgcccgcacc atcaatcacc agtggaccag gctggttgat gagtgcttcc
     4741 gaggctggcc cgttatctcc acgatagcca aactcattgc caccaacaac agttgaaatc
     4801 attccggttc gggcatcaag gcggcggata cggttatttg gaggatcaga aataaacaga
     4861 ttcccttccc ggtcaatggc taaagatccg ggggagaacc gtttcccttt gatcagttcg
     4921 cttccgacct gtctggctga aaccagcgtg gaaataagac cggtccgaag atcaacccgg
     4981 cgaatctggc tgttgttcgg atcagcgatc aggagctggt tgttgatcag gtcaggtaac
     5041 accgcccagg gttccaggct ggcggcagtg gctggtccgc catccccaga ggcaccagat
     5101 ttgccattgc cggcaatcgt tgtaatcaag ccagtttgag cagttacctt cctgacgcga
     5161 agtgatgcac tatcaacaat atacagatta tctttctcat caaccccaag ctggctcacg
     5221 gtatcaaacg aagctttcgt tgccggtccg ccatcaccct ttgattcaat tttgccattt
     5281 ccagcaaaga gagcgacgcg accagtgtgc gcatctactc gaaagacctg acgtgttacc
     5341 aaatcagaaa ccagaatatc tgcagtcgaa gtaaccgcca catcggttgg aatctggaga
     5401 aaatagctgg tggccagatc accctcacga cgttcgatgg caatgccatt tccagccaca
     5461 gtggtaatga tatcggtttg ggcatccacc cggcgcaggc ggttattgaa actgtcgcaa
     5521 atgaataagt tt
//
LOCUS       DAOQDO010000078         5527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9199_length_5527_cov_2.969481, whole genome shotgun sequence.
ACCESSION   DAOQDO010000078 DAOQDO010000000
VERSION     DAOQDO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9199_length_5527_cov_2.969481"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKZ53_02090"
     CDS             complement(<1..725)
                     /locus_tag="PKZ53_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNJ39251.1"
                     /translation="MCGIVGYVGQKQVIPVLLDGLKRLEYRGYDSAGIAVVDNGQLNI
                     RRASGKLRNLEEVIRLNPLDGTYGIGHTRWATHGRPTEENAHPHCDASRRIVVVHNGI
                     IENYLPLKRRLQAEGHTFVTQTDTEVVAHLVGKYRRETDSLEEAVRMAVHELTGIFAI
                     TLLSADEPDKVVTARMGPPVVVGLGKDEYFVASDIPAILYHTRDVFFLGDGEMAVLTR
                     SGVMVSDFDGNLVTPNIQRITWDP"
     gene            complement(1217..1453)
                     /locus_tag="PKZ53_02095"
     CDS             complement(1217..1453)
                     /locus_tag="PKZ53_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNJ39252.1"
                     /translation="MASIADKYADNATGMYYVDNQCIDCDVCRDTAPSNFTRNDDGGY
                     SYVYKQPDTDEEFEQCKEAMEACPVEAIGDDGEQ"
     gene            2073..2435
                     /locus_tag="PKZ53_02100"
     CDS             2073..2435
                     /locus_tag="PKZ53_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39253.1"
                     /translation="MTFDKLLDGILDKVYGARGVVFLAQDGEIVYQCAQRKVTNLDLL
                     AAYQGITMTTCQRFADDFGIGHISTVVLNYEAGACVLKLLNEGYILMVSLDADGNIGQ
                     TMHLVNSVTDQLNAEIGY"
     gene            complement(2633..4441)
                     /locus_tag="PKZ53_02105"
     CDS             complement(2633..4441)
                     /locus_tag="PKZ53_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ39254.1"
                     /translation="MIPTLLNNRYQVVQSLSGGGYSHTFLAEDTHLPSNRRCVIKQLK
                     PLSTDPDTYRIIQERFQREAAVLEALGKQNDQIPELYASFTENGQFYMVEEWVEGKTL
                     TQIVRERGAFSENEVRDLLVHLLPVLEFIHTQRIIHRDIKPDNIIIRNRDGKPVLIDF
                     GVVKEVIDVDALGNPTSTIMIGTKGFMPLEQAAGKPVYASDIYGLGMTSICLLTARTP
                     QHLTDPETGEMVWYRYAPHLTPTFAHILKRSTEQLPRDRFSSAREMLEALTPGTGAAA
                     MRTTASHSVFDPKTLPTTVTNSNMASFPFVTATVDQNAVITRSPETHGSVFQEEIVDG
                     LDVELVAIAGNTFLMGTDDFGIERVTQEYERYGIKSPGVVKQLRSETPQHYVTVAPFM
                     MSRYQITQAQWRMVASFPRVNIDLNPDPSFFKGDDRPVEQVSWEDAIDFCARLTRRTG
                     RSYRLPSEAEWEYACRAGTTSPFAFGETITTDLVNYDGNFPYGNAPKGLERRQTIFVG
                     SLGLANAFGLSDMHGNVWEWCLDVWHFNYIRAPEDGAAWTAGGDPQYRVVRGGSWNSF
                     GYLCRSGLRFRNSPVNRYNNLGFRIVLDFGQIPTRW"
     gene            complement(4447..>5527)
                     /locus_tag="PKZ53_02110"
     CDS             complement(4447..>5527)
                     /locus_tag="PKZ53_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ39255.1"
                     /translation="NSEVNLNELASMTRGFVGADLHSLCREAAMAALRRTILAVPDVD
                     SSLIEEHLSRMRVLMDDFHQAMKLVEPSGLRGIVVEAPEVSWAQVGGLDQVRSELTRA
                     VLFPLLHAEAFSRLGVRPTRGVLLTGPPGSGKTLVARALAHAGGTNFISVRSSELIVR
                     YATESERVIEDIFRQARQAIPCIVFIDEIDLLMPGHTGDRSSDTAIQERILAQLLTEL
                     DGIEELSGIIVIAATNRLDRIDVALRRPGRFDVVQRIPLPDVDGLKAIFEVYLEGRPL
                     GADVDIAALAYQAQGMTGADIEAVCRLAATQTIEQATASGNFPNSVTPLCFTHPILSE
                     AIESVRRMRYNKQTFFDPSSGTDSQ"
BASE COUNT         1326 a         1483 c         1406 g         1312 t
ORIGIN      
        1 ggatcccagg tgatccgctg gatgtttggc gtcaccaggt tgccgtcaaa atcgctgacc
       61 atcacacctg accttgtcaa caccgccatt tccccatcac ccaggaaaaa cacgtcccgc
      121 gtgtggtaca aaatggctgg aatgtctgaa gccacaaaat attcgtcttt ccctaagcca
      181 accacgactg gcggccccat ccgggcggtg accactttat ctggttcatc ggcagacagc
      241 agcgtaatcg caaaaatccc ggtcagctcg tgaaccgcca tccggacggc ttcttccagt
      301 gaatcagtct cacggcgata cttgccaacc agatgtgcca cgacttcggt gtcggtttgg
      361 gtgacaaagg tgtgaccttc ggcctggaga cgccgcttga gcggcaggta attttcaatg
      421 attccgttgt gaacgacgac aatccgccgc gaggcatcac agtgcggatg cgcgttttct
      481 tcggttggcc ggccatgggt ggcccaccgg gtgtgaccga tcccataagt gccatccagc
      541 ggattgagcc ggatgacttc ttccagattt cgaagctttc ctgaagccct ccgaatattg
      601 agctggccat tatccaccac ggcaatcccg gctgagtcgt acccgcgata ttccaaccgc
      661 ttcaggccgt caagcaacac cggaatcact tgcttttgtc caacataccc aacgattcca
      721 cacatttttt tcttgaaatc cttcttgaaa gattgagaga gggaacccac tcatctctca
      781 gtatgaatga tgagtggtga attatgaaga agcttccacc aatcttgggg ctgaaaaacc
      841 ctcgggctca gggcttgggg ctgaaaaact cttgggctca gggcttgggg ctgaagactc
      901 gcaagctcgg ggcagaagaa agcgagttca atcccctgag cccaatgttt tcagcccgtc
      961 ttcttcagcc cgtcttcttc agcccgtctt cttcagcccg tcttcttcag cccgttgtct
     1021 tcagcccgtc ttcttcagcc cgttgtcttc agcccaaaat cttcagcccc agggttttcc
     1081 ccaaacacaa aacccgaacc accatctgat ccaactgaag ggaggttcgg gtcttgggcg
     1141 tttcactatc aatttttgaa aactgatgct gggactgcgg tgcttgaaaa atcagcgcga
     1201 gttgccaggt agggagttac tgctcaccat catcgccaat tgcttcgacg gggcaggctt
     1261 ccatggcttc cttgcactgc tcgaactctt catccgtatc aggctgcttg taaacgtagg
     1321 aatatccgcc atcatcgttg cgggtaaaat tgctgggtgc cgtgtcacgg cacacgtcgc
     1381 agtcgatgca ttggttgtct acatagtaca ttccagtcgc attatctgcg tacttgtctg
     1441 caatactggc cataaaaatt tcacctcaaa gtcaaaaata gagtcagtcc tgaggggctg
     1501 gccaaagtta cctgtcaacg ctctcaagca acttgagcaa actggcgcct ctgtcctgtg
     1561 ttgtgcgtct tcgacgcggt gacgtcgatg agaaaatgca agttgtgggg cagaaaataa
     1621 ctatcaaggt caaagactgt gggcttttct gcctcaggtc ttttttcaat acttatattg
     1681 aaaaaatacc tttaattgtc aagccctctt gcgctgaaac tcacgcttcg caacctcaat
     1741 ccatcacccg gcgtgcggca aaaagaaaga acagatcccg ttctatcggg gtttttcaca
     1801 aaaaatcacg actcgaaaag ggatgtccgg cacaggtaaa acagggaggg cggcatgaaa
     1861 ggcgagaaca taccacgagc gttcaaaaac gaaaaggacc cggtgagttt ttttagcagt
     1921 cacgggttcg ttcctgatgc tttgcatctg gactgagctt atgctattta gaccttctct
     1981 catttcacgg ttcatcaagc tggtttccat cgccccggtt catctcacac ttcacattcc
     2041 gcaacacacg tttgcttcac ctgagagcac atatgacttt tgataaacta ttggatggaa
     2101 ttctggacaa ggtatatggg gcaagggggg ttgtttttct ggcgcaagac ggagaaattg
     2161 tctatcaatg cgcccaacgc aaggtcacga acctggattt gcttgcggcc taccagggca
     2221 tcacgatgac cacctgtcag cgctttgctg atgactttgg catcggccac atttcaacgg
     2281 tggttctcaa ttatgaagcc ggtgcctgtg tgctgaaatt gctcaatgaa ggctatatcc
     2341 tgatggtctc actcgatgcc gatggaaata tcggccagac catgcatctg gtcaattcag
     2401 tgacggacca actcaatgct gagattggat attgagggaa gtggcgaggg agttcagcgt
     2461 tgctgcttct accgattttg tgttttgccg ggttagcatt gaaaataaag gacctgtcag
     2521 taccacctgc gtgagcgggt gggattggag gcaaaacaga cccacccact gacgcgggcg
     2581 gtactgactg agttgccttt ctgattgcta cttcacgact tcactcaaac aactaccatc
     2641 gggttggaat ttgtccaaaa tcaagcacaa tccgaaagcc aaggttgtta tagcggttga
     2701 ccgggctgtt tcggaacctc agaccagatc ggcacaaata cccaaaacta ttccacgagc
     2761 caccgcggac cactcggtat tggggatccc cgccagcggt ccaggcagcg ccgtcctcag
     2821 gtgcccgaat gtagttaaaa tgccagacat ccaggcacca ttcccacaca ttgccgtgca
     2881 tatctgacag cccaaaagca ttggcaagcc cgaggcttcc gacaaagatc gtctggcggc
     2941 gttcgagtcc tttgggggca ttcccgtatg gaaaattccc atcataattg accagatcag
     3001 tggtgatcgt ttccccaaag gcaaaaggtg aggtcgtacc agcccggcag gcatattccc
     3061 actctgcttc gctcgggagc cgatagctgc gcccggttcg gcgggtcagg cgggcacaaa
     3121 aatcaatagc atcttcccac gaaacctgtt ccaccgggcg gtcatcgccc ttaaagaatg
     3181 acggatccgg atttaaatca atgttgaccc gtgggaaact tgccaccatg cgccactggg
     3241 cctgagtaat ttgataccgg ctcatcataa acggcgcaac cgttacataa tgctgtggcg
     3301 tttcgctgcg gagctgcttc acgacgccag gtgatttgat accatagcgt tcatattcct
     3361 gagtgacgcg ttcaattcca aaatcgtcag tccccatcag gaacgtgttt ccggcaatgg
     3421 cgaccagttc aacatccaga ccatcaacaa tttcttcttg aaaaacactg ccgtgcgttt
     3481 caggcgaccg ggtgatgacc gcattctggt caaccgtggc cgtcacaaac ggaaaactgg
     3541 ccatgttgga gttggtcacg gtggtgggca aggttttggg gtcaaacacc gaatgtgagg
     3601 cggtggtgcg catcgcagcc gcgccggttc caggcgtcag ggcctccagc atttcacggg
     3661 ctgaagaaaa ccgatcacgg gggagttgtt cggttgatcg cttgaggata tgcgcaaacg
     3721 tgggagtcag atgtggcgca tagcgatacc agaccatttc cccggtttca ggatcggtca
     3781 aatgttgcgg tgtccgggcg gtgagcaaac aaattgacgt catccccaga ccatagatat
     3841 cgctggcgta gacgggtttt cccgctgcct gttcaagtgg cataaaccct ttggtcccaa
     3901 tcataatggt gctggttggg tttccaagtg catccacgtc aatcacttct ttgaccacac
     3961 cgaaatcaat caagacgggt ttgccatcgc gattgcggat gatgatgtta tcgggcttga
     4021 tgtcacggtg gataatgcgc tgggtgtgaa taaattccag gactggcagg agatggacga
     4081 ggaggtcgcg aacttcattt tcgctaaatg cccctcgttc acgcacaatc tgggtcagag
     4141 ttttgccttc aacccactct tcgaccatat aaaactgccc attttcagta aatgaggcat
     4201 acagttcagg aatctggtcg ttttgcttgc ccaatgcctc cagaaccgca gcttcccgtt
     4261 gaaaacgttc ctggataatg cggtacgtgt ctgggtcggt tgagaggggt ttaagctgtt
     4321 tgatcacaca tcggcggttt gacggcaggt gggtatcttc cgccaaaaac gtgtggctat
     4381 acccaccacc gctgagggat tgaacgactt gatagcgatt attgagaagt gtcggaatca
     4441 ttcaagttat tgcgagtcag ttccggatga gggatcaaaa aaagtttgct tgttgtaccg
     4501 catccggcgc actgactcaa tagcttcact caaaattggg tgtgtaaaac aaagtggggt
     4561 tacactattg ggaaaatttc cagaggcagt tgcttgttcg atggtttgag tggcggccaa
     4621 tcggcaaacg gcttcgatat cagccccggt catcccctgg gcctgatagg ccagagcggc
     4681 aatatcaaca tctgcgccga gtgggcggcc ttcaaggtaa acttcaaaaa tggcttttaa
     4741 gccatccaca tctggcagag gaattcgctg aaccacgtca aagcgtcctg gccgacgcag
     4801 tgccacgtca atccggtcaa gtcggttggt ggcagcaata acgataattc cgctgagttc
     4861 ttcaatgcca tccagttcgg tcagaagctg agccaggatc cgttcttgaa tcgccgtatc
     4921 cgatgaccgg tccccggtgt ggccaggcat cagaaggtca atttcatcaa taaaaacaat
     4981 acagggaatc gcctgacgcg cctgacggaa aatatcttcg atgactcgct ctgactccgt
     5041 ggcatatcgc acaatcaact cagagctacg caccgaaata aaattggtac caccggcgtg
     5101 ggctaaggcc cgagcgacca gtgttttccc gctccctggc ggcccagtca gcaagacgcc
     5161 ccgtgttggc cgaaccccga gccgactaaa cgcttcggcg tgcagcaggg gaaagagcac
     5221 tgcccgggtg agttccgagc gtacctgatc aagtcctcca acctgggccc agctcacttc
     5281 tggcgcctca accacaatgc cccgcaaccc tgaaggttca accagtttca tcgcctggtg
     5341 gaaatcgtcc atcagaaccc gcattcgcga caaatgctct tcaatcaaac tggaatccac
     5401 atcaggtacg gccaggatgg tacgtcgcag tgccgccatg gcagcttcac ggcacaaact
     5461 gtgcaaatca gccccgacaa aaccgcgcgt catgctggct aactcgttga gattgacctc
     5521 tgaattg
//
LOCUS       DAOQDO010000079         5525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9206_length_5525_cov_3.134004, whole genome shotgun sequence.
ACCESSION   DAOQDO010000079 DAOQDO010000000
VERSION     DAOQDO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9206_length_5525_cov_3.134004"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1384)
                     /locus_tag="PKZ53_02115"
     CDS             complement(86..1384)
                     /locus_tag="PKZ53_02115"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNJ39256.1"
                     /translation="MARKKFRMGHTNRREPETERSFFEHMFTRDNHQTLDRREVLDLV
                     RGGEDSEVEFKSRFSNPEKIAAEIVALANTGGGAILFGVSDTCRIEGLDNADRVEEEL
                     REVCAHDIIPPIHPYMDKVSFDNGRRVLVLEVDDRRAPHTTRDQKFYIRVGSVKREAE
                     LDEIPELFRRYRPTHFEQIPWFPASIDDIDESLVWAYVKAVQGDTGKLPAGYPTAMVL
                     QDMQLAITRADEYIPNLIGLLLFGKSKSVARLFPRSRLVATRFSGDAVTDPIVEQVAF
                     TGNIGTLSEYAEGFLNRYVDLTDQPLYRKSASHQGPTSHRRANYSRKAVMEAVTNALV
                     HRDYSAREECIRLVVFDRRIEVANPAFAKGLPRKAVELYGVSFPTNPRLKAFMKNEAY
                     GATIHHGGLPMIRREMHHFTRREPKISIHAAEFQVEIFGA"
     gene            complement(1440..2033)
                     /locus_tag="PKZ53_02120"
     CDS             complement(1440..2033)
                     /locus_tag="PKZ53_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNJ39257.1"
                     /translation="MLYTQILTKLLILLVVATGMVCVVAGESVFAGAGPVAEKKDERD
                     EPQLTLRRIRVEKLRIRTADLIASVDILNPKSAVTIKNLKFNVKLNGVQSGVGEYEDT
                     LKLPSKSTLSIDLPLTVDLMALPEVGLNGLLDGAQLEYEIRAEFDVPVLFFKKHVERK
                     LRGTVPLESLAPKLDLPELDLPRIELPRLRIPRIDWP"
     gene            complement(2134..4812)
                     /gene="polA"
                     /locus_tag="PKZ53_02125"
     CDS             complement(2134..4812)
                     /gene="polA"
                     /locus_tag="PKZ53_02125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNJ39258.1"
                     /translation="MAKKLFIVDGMAHIYRAFFAVRGLATSTGIPTNAVFGFISILRK
                     VIGDYKPDYIVVAMDSKEPSFRQELYAEYKANREVMPEDLQTQIPYIFKACDVLGVTT
                     ARVSGFEADDLIGTLARKATEQGIDTVIVSNDKDMTQLVNDHVSILRLDNKSGKMILC
                     GKAEVVEWMGVEPEKVVDVLSLWGDSSDNIPGAPGIGEKGAVQIIQQFGTLDAALEGF
                     AEVKRRVYSESLRDHRDRILLAKQLVTIHQDAPVDLDLAAVAARPPDTAAAYALFAEL
                     EFKALMREFSAGATAARAAAPVQTIEVKRTYRQISKADEALKFIDSLFAHDSFAIAVD
                     EQNGAFGGCSVSLGPGQAVKLDQELILNSLQVGEALAEMLENGLVTKQVHDAKRALYT
                     LNRPPHAYGPGEDLLSQTVEGLPFGKKVRFEGLNDDVMLAAYLLNANLKQEEYALPAL
                     ALNYLGVGETEFKDHDPADLTSRLARMLRGRLEMDELTSIYETMELPLVDVLFDMECA
                     GVKIDPQPLTDLEKEIHVALDRLTGQIHSLAGQEFNINSPQQVGEIFEKLNYDVSKKT
                     TTGKISTSAEVLEELAETYELPRLILEFREYEKLLNTYVTVLPRMVNGQTHRLHTTFN
                     QAATATGRLSSTKPNLQNIPIRTEFGRRTRRAFVAEDGNILIAADYSQIELRLLAHIT
                     GDPVMTEAFLHNEDIHTKTARAVFGAQTPEELKQARRLAKATNFGIAYGVGAFGLARN
                     VGISRKEAKKAIDSYFATYPGIKRYMEETPEKGRETGYVRTMFGRMRRFPDLTSRNYT
                     VRSRAEREAINAPIQGAAADLMKLAMIRIAEQFKTEDLKTRMLLQVHDEILFEAPKDE
                     AEHVATIVKETMETVHQLNVPLVAEVHMGDNWLDAK"
     gene            complement(5128..>5525)
                     /locus_tag="PKZ53_02130"
     CDS             complement(5128..>5525)
                     /locus_tag="PKZ53_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ39259.1"
                     /translation="GLDLLIVDYLQLIRGKGRFENRQTEVSQISRDLKALAKELNIPV
                     IALSQLSRASETRSDHRPQLSDLRESGSIEQDADVVGFIYRDEVYNATEENAGLAELI
                     IAKQRNGPTGTANFVFIKEFTRFDNPWRE"
BASE COUNT         1265 a         1492 c         1378 g         1390 t
ORIGIN      
        1 atcagttagc tctttattct caatccttta tcctcagccc acccactgac acaggtggta
       61 ctgacttctc attcttcatt cccccttacg ctccaaaaat ctcaacctga aattcagccg
      121 cgtgaattga gattttcggt tctcggcggg tgaagtggtg catctcgcgg cggatcatcg
      181 gcagccctcc gtgatggatt gtggcgccat aggcttcatt tttcataaag gctttcaacc
      241 ggggattggt cgggaagctc acgccataga gttccacggc tttgcgcggt aaccctttgg
      301 caaaggccgg gttggcaact tcaatccgcc gatcaaatac caccagtcgg atgcactcct
      361 cacgggcgct gtaatcccga tgcaccagcg cattggtcac ggcttccatc acggctttgc
      421 gggaatagtt ggcccggcga tggctggttg gcccctggtg cgaagcgctt ttgcgataga
      481 gcggctggtc ggtcaaatca acgtagcgat tcaaaaagcc ttcggcatat tccgaaagcg
      541 ttccaatgtt tcccgtaaag gcaacctgtt caacaatcgg gtcggtgact gcatcgcctg
      601 aaaaccgtgt cgcaaccagc cggcttcgcg ggaaaagccg tgccacgctt ttgcttttgc
      661 caaacagcaa caacccaatc aaattcggga tgtattcatc ggctcgggta attgccagtt
      721 gcatatcctg caacaccatc gccgtcggat accctgccgg aagtttcccg gtatctccct
      781 gtacggcttt gacatacgcc cagaccagcg attcgtcaat atcatcaatc gaggctggaa
      841 accacgggat ttgctcgaaa tgagttgggc gatagcgacg gaaaagttcg gggatttcgt
      901 cgagttcggc ttcgcgtttg acggagccaa cccggatata gaatttctgg tcgcgggtgg
      961 tgtgcggcgc ccgccggtcg tcaacttcga gcaccagcac ccgccggcca ttgtcaaacg
     1021 acactttgtc catataggga tggatcggcg gaataatgtc gtgcgcacag acttcgcgga
     1081 gttcttcctc aacccggtcg gcattgtcga ggccctcaat ccggcaggta tcactcacgc
     1141 caaataaaat cgcaccacca ccagtattgg cgagtgccac aatttcagcc gcaatttttt
     1201 cgggattgct gaaccggctt ttaaactcaa cttcgctgtc ttcgccgcca cgaaccagat
     1261 ccagtacctc tcgccggtca agggtctggt ggttgtcgcg ggtgaacatg tgttcaaaga
     1321 aagagcgttc ggtctctggc tcacgccggt tggtgtgacc catgcgaaat ttttttcgag
     1381 ccatacaatt tgcgcgatgc gcggggggtg atgaaatatt gatccaccct ttgaaaagat
     1441 cagggccagt caattcgggg gattcgcagg cgtggcaact caatacgcgg cagatcaagt
     1501 tccggcaaat cgagctttgg cgccagggat tctaatggaa ccgttcctct gagttttcgc
     1561 tcaacgtgtt ttttgaagaa taaaacaggt acgtcaaatt ccgcacggat ttcatattca
     1621 agctgggcgc catccagcaa accattgagg ccaacttccg gaagcgccat taaatcaacc
     1681 gttagcggta aatcaatcga aagcgttgat ttggatggaa gcttcagtgt gtcttcgtat
     1741 tccccaacac cgctctgcac cccgttgagt ttgacattaa atttgagatt tttgatggtc
     1801 acggctgatt ttggattgag aatatccaca ctggcaatca ggtctgccgt tcgaatccgc
     1861 aatttctcca ctcgaatgcg ccgcaatgtc aattgtggtt cgtcacgctc gtctttcttt
     1921 tcagcgacag ggcctgcccc cgcaaacacc gactcaccag ccacaacaca caccatgcct
     1981 gttgccacaa ccagcaaaat cagtaatttg gtgagtattt gagtgtacag catagggttt
     2041 tattagcgcc aggaggagag gttttaggcc cgaagggtcg ttgtgtaata gccgtggtgc
     2101 aaagcccagg gtcctggcca gaaccccgga accctacttc gcatccagcc agttgtcgcc
     2161 catgtggact tcagccacga gaggaacgtt cagttgatga actgtttcca tcgtttcttt
     2221 gacgattgtg gccacgtgtt cggcttcatc tttgggtgct tcaaacagga tttcgtcgtg
     2281 aacctgtagc agcatccggg ttttcaggtc ttcggttttg aactgttcgg caatgcgaat
     2341 catcgccagt ttcatcaaat cagccgcggc cccttgaatc ggagcattga ttgcttcgcg
     2401 ttccgcccgt gaacgcacgg tgtaattccg actggtcaaa tctggaaagc gccgcatccg
     2461 tccaaacatc gtgcgaacat acccggtttc acgtcctttt tccggtgttt cctccatata
     2521 gcgcttgatg ccgggatagg tcgcaaagta gctatcaatc gcttttttgg cttccttgcg
     2581 gctaatccca acgttgcggg ccaaaccaaa agcaccgacg ccgtaggcaa taccaaaatt
     2641 ggtcgccttg gccagccgcc gagcctgttt gagttcttcc ggtgtttgtg caccaaacac
     2701 cgcacgcgca gttttggtgt gaatgtcttc gttgtgcaga aacgcttcgg tcatcaccgg
     2761 gtcacccgtg atatgagcca gcaagcgaag ttcaatctgt gaatagtcag ccgcaatcag
     2821 gatgtttccg tcttcggcca caaatgcccg gcgagtacgg cgtccaaatt cggttcgaat
     2881 tggtatattt tgaaggttcg gtttggtgga tgacagccga ccggttgcgg ttgcagcctg
     2941 gttaaaggtt gtgtgtagcc gatgggtctg accgttgacc atacgtggca aaacagtcac
     3001 ataggtattg agcagtttct catattcgcg gaattctaaa atcagtcgtg gcaattcata
     3061 ggtttcggcc agttcttcga gcacttcagc actggtcgaa atctttccag tggtcgtttt
     3121 cttcgacacg tcatagttga gcttttcaaa gatttcccca acctgttgcg gcgaattgat
     3181 gttgaattcc tgtccggcaa ggctatgaat ctgacccgtc agccgatcca gagccacgtg
     3241 gatttccttt tccaggtctg tcagcggctg cgggtcaatt ttcaccccag cacattccat
     3301 atcaaacaac acatcaacca gcggtaattc catcgtctca taaatgctgg tcaactcgtc
     3361 catttccagc cgtccacgga gcatccgtgc cagtcgggaa gttaaatcag ccggatcatg
     3421 gtctttaaac tctgtttcgc caacgccaag gtaattcaaa gccagcgccg gcagggcata
     3481 ctcttcctgt ttgagattgg cattgagcaa atacgcagcc agcatcacgt catcgttgag
     3541 cccttcaaac cgaacctttt tcccaaacgg caagccttcg acggtttggg aaagcaaatc
     3601 ttcgcctggt ccataggcgt gcggaggccg gttcaacgta tagagcgcac gcttcgcatc
     3661 gtgaacctgt ttggtaacca gtccattttc aagcatttcg gccagggctt caccaacttg
     3721 aagcgagttg agaatcagtt cctggtcaag cttcactgcc tgccctggtc ccagcgaaac
     3781 cgaacatccg ccaaatgcac cgttctgctc gtcaaccgca atcgcaaagc tatcgtgggc
     3841 aaacaggcta tcaataaatt tcagggcttc atcggctttg gaaatttggc gataagtccg
     3901 tttgacttca atggtttgca caggtgcagc cgcccgtgcg gcagtggcgc cggcggaaaa
     3961 ttcccgcatc agggctttga attcgagttc ggcaaagagc gcatacgccg ccgccgtatc
     4021 tggcggacgc gcggccactg ccgccaaatc aagatccacc ggtgcatcct ggtggatcgt
     4081 caccagttgt ttggccagca aaatccggtc acgatgatcg cggagcgatt cgctatacac
     4141 acgacgcttg acctcggcaa aaccttccag cgcagcgtcc agtgtcccaa actgttgaat
     4201 aatttgaacg gcgccttttt ccccaatgcc gggtgctccg ggaatgttgt cagagctatc
     4261 gccccataac gacagcacat caacgacttt ttccggttca actcccatcc attcaaccac
     4321 ttcagctttc ccacagagga tcatttttcc gcttttatta tccagccgga gaattgaaac
     4381 gtgatcattg acaagctggg tcatgtcttt gtcattactc acaatcacgg tatcaattcc
     4441 ctgttcggtg gctttgcggg ctaaggttcc gatcaaatca tccgcctcaa aacctgacac
     4501 ccgggccgtc gtcacgccca gcacgtcgca ggctttgaaa atgtagggaa tctgcgtttg
     4561 caaatcttca ggcatgactt cgcggttggc cttgtattcg gcatagagtt cctgccgaaa
     4621 cgacggttct tttgagtcca tcgcgaccac aatgtagtca ggtttatagt caccgatcac
     4681 tttgcgcaga atcgagataa acccaaaaac agcattggtt ggaatgccag tgctggttgc
     4741 cagtccgcga actgcaaaaa aagcgcggta gatatgcgcc atgccgtcaa cgatgaagag
     4801 ttttttggcc atatattttg gggaaatgaa gtgagtcagg ttaaaaacat tgggctgaag
     4861 acatcgggct tggggctgaa gacattgggc ttggggctga aacacccaat tttcttcagc
     4921 cctgagcccc aagccctgag cccaatattt tggggaaatg aagtgagtca ggttaaaaac
     4981 attgggctga agacatcagg cttggggctg aagacattgg gcttggggct gaaacaccca
     5041 attttcttca gccctgagcc ccaagccctg agcccatctt cttcagccca ctttcttcaa
     5101 cccaccgtct tcagccccaa caattcacta ctcgcgccag ggattatcaa agcgggtaaa
     5161 ttctttgata aaaacaaagt ttgccgttcc agttgggccg ttgcgctgct tggcaatgat
     5221 gagttcggcc aatccggcat tttcttcggt tgcattgtac acttcgtcac gatagataaa
     5281 accaaccacg tcggcatcct gttcgattga tccgctttcg cgcaggtccg aaagctgtgg
     5341 tcggtgatca gagcgggttt ccgaagcccg gctaagctgg gataaggcaa tgaccgggat
     5401 gttgagttct ttggccagtg ctttgaggtc acgggagatt tgcgacactt ccgtctggcg
     5461 attttcaaac cgccccttgc cgcgaatcaa ctgtaaatag tccacaatca acaaatccag
     5521 tccat
//
LOCUS       DAOQDO010000080         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9230_length_5516_cov_2.585058, whole genome shotgun sequence.
ACCESSION   DAOQDO010000080 DAOQDO010000000
VERSION     DAOQDO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9230_length_5516_cov_2.585058"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..514)
                     /locus_tag="PKZ53_02135"
     CDS             complement(314..514)
                     /locus_tag="PKZ53_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39260.1"
                     /translation="MVHIRFEGRSYDFQPQAANIRPTTTDADVLRRVATLLNVAPERL
                     KNYVVDRTANGTVIIRPEAVYG"
     gene            1144..3567
                     /locus_tag="PKZ53_02140"
     CDS             1144..3567
                     /locus_tag="PKZ53_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39261.1"
                     /translation="MQAIFQDLRHCVRLVRRNRLFAATVIGFLGLGIGINIIFFSLVN
                     SVFLKPLPFADADRLLVVAEQSEKEPDARLRVSIPDYLDWSSANPVFDRMGAWSPVSF
                     NIAEGGEPEYVPGQVVSSTLFSTLGVKPLYGRTFEAREDQEGQGLSLVLSHGYWVRRF
                     GADPGIVGRVVALNGVGFTVVGVLPAEFAFFQPADVWVPMGVHGGNLRIWRATHLYHV
                     MGHLKPGVTPQQANSEMDALARRIWNQDTLFTGFGTSSIPLRSYLYGDLGLPLLAVWL
                     AGVLILLIVCVNVGLIVLAHSLGREREFSIRQAIGASRWRLVQQFLTESVFLSLLGGL
                     AGLGLALLSLETVHHVLAPFFPDFQKLTFDQTVWLQTAGLIFLVGVGLGCSPAVRLVK
                     SNLNFHLRQSRGQGQSHGRLISGRNTLIVVEIAVSLVLLAGGVLVSRSLINLIQVDPG
                     IQAENRLVFRINLPPAKYAEDSLKVSLLSRFEADLKKLPGVKAVGMGSNYPFSPTAVM
                     PATSIFIEGRDNRDMNNVPLADTRTINAGYFSALGINLVAGRDLNERDTEGTQPVAII
                     NEMMARRFWPGQNPLGARLRSGDSPNPDAVYTVVGVVEDVKNRGLRNETLQEVYFSYR
                     QRPPRSMAFVVSGHIDPLTFAGTIRSQLAQLDPGLALFEMASLGELVRQSLMVPKLTA
                     WVLGSIAGVAFFLSLIGMFGVITFSVKQRTFEFGLRLALGAHPNQIVGMVVRQGLWLA
                     VLGLVAGIFLASFALQILNPLLFGIETFDVPTLVCISVAIVGLTTLASYLPARHAAAI
                     NPLIALRSE"
     gene            3579..5288
                     /locus_tag="PKZ53_02145"
     CDS             3579..5288
                     /locus_tag="PKZ53_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39262.1"
                     /translation="MNYSKLLCGSVCTGILVSLLSCTLYPQFQSASAKAQPQSQNVSR
                     ATIKEAQALFAKQEWAKTAAMCEELVKTNPYNGAIWDMLGFAHHQLKHFDQAIPAFQK
                     AAECGTNPASELYNIACGYALKGDKEQALVWLEKAMKARFSDFETIRNDSEIDSLRSD
                     PRFKKLVGEKPDGTLSRDEQWRYDLQYLARELKRLHYNLYNKLDQARFEAIVAEIDRK
                     IPTLKDHQIIVELVCLAAAIGDGHTLFMPPTSGEGGFHKVPVQFSEFKEGIFVTAAAD
                     EYKAALGMKLSKIGKTPVEQAYQMISKTVSRDNDIWLRMQVPRRFGVPEYLHAFGMIP
                     SLDEVSYVLTDAQGKELTISPKPRPFLEQVNLISVRPSGSDVTPLYLKDQENLYWYEY
                     LKDSKTVFFQYNGVQNKPEESIAAFVKKMFAFINANEVERLIIDLRWNGGGNNFLNKP
                     LVQEIIKCEKINQPGKLFVIMGRNTFSAAICGASDLQQQTQAMFVGEPSASPPNFIGE
                     STMTTLPFCKLRASISSLYWQNAPAMDYRVWIAPDLAAEPTYEAFVANRDLALEAIFN
                     YGK"
BASE COUNT         1269 a         1306 c         1430 g         1511 t
ORIGIN      
        1 gtaagctttt tctgtcggag tacaggacag aaatcaattt tcaaaaaaac aaaaaaggaa
       61 ttgtaaatcg ctaacctgtt gaaaataatg ctttattgaa gactgatgac tgacgactga
      121 cttgtgattg atcatctcca cgcaagttac tgaagcggtt gtcactacat tcagtgtgta
      181 tccatttcac cacaaaccca aatggtggat ttgacaggag tcgaacctgc agagcccgtg
      241 agggccgctg aattcaattg agtgtgtatg cttcaccggt cagagcgcgg agatgaaaag
      301 atcaaaggtt catttagccg tagacggctt ccgggcgaat gatcaccgtg ccgttggccg
      361 ttcgatccac aacgtagttc ttgagtcgct ctggggcaac attgagcagt gtcgcaaccc
      421 ggcgtaaaac atcagcgtcg gttgtggtcg gtcggatatt ggccgcctgt ggctggaaat
      481 cataggaccg tccttcaaat cgaatgtgta ccatagggtg gtggctcctt ttgagtcggt
      541 ttgatcagca ctgagcggct gttctctgac tgtgagtaat ggcattctgt gtgccagttc
      601 ctcaggtaaa atcacagatt ttctataaaa tattgaaaat acagggttaa aaattttcct
      661 aaagttttac ttgcgatatt tttttgctag attgggtgaa gatttttaga aaattagata
      721 gttttctttg aattttatcg agtactgagt tgatttgttc aaccccggcc tggttggaat
      781 attcgcaccc cattgttcga aactgggaaa ctgtcgtgtc acgagcgaac acaaaaatga
      841 gatcgcttta cgacggtttt gtatatggag aaatgccgta accagttcaa ccacaacaat
      901 ttgaattcat tactcttcat ttttcacaaa ccggcacatc acttgcagtt ctgagttggt
      961 ttcaacctcg ttttgacacg ctttttgaat tcattttgaa ctaagccagt ccgattctca
     1021 ttaacctatc ttattgaaaa acaaaagctt tattttttct tcattctcaa ttcttcattc
     1081 ttcattctca attctttatt cgcctcaggc catcaaatca gatcaacaca aaggaatttt
     1141 gctatgcagg caatctttca ggatcttcgg cactgcgttc ggttggtgcg ccgcaatcgc
     1201 ctgtttgcgg caactgtgat tgggtttctt ggccttggca tcggtatcaa catcatcttt
     1261 ttcagtctgg tcaattcagt ttttctcaaa ccattgcctt ttgctgacgc tgatcggttg
     1321 ctggtcgttg ctgagcaaag cgagaaggag ccggatgccc gacttcgggt ttcaattcct
     1381 gattacctgg attggagttc agccaacccg gtgtttgatc ggatgggagc ctggtctccg
     1441 gtcagtttca atattgccga aggtggtgaa cccgaatatg tccctggtca ggtggtgtcc
     1501 tcgaccttgt tttcaacatt gggtgtgaaa cccctgtatg gacgaacgtt tgaagctcgc
     1561 gaagatcagg aaggtcaggg gttgtcgctg gttctcagtc acgggtattg ggtccggcgc
     1621 tttggtgctg accctgggat tgttggccgg gtggtggcac tcaacggcgt tggatttacc
     1681 gtggttggcg tgttaccggc tgaatttgcc ttctttcagc cagcggatgt ctgggtgccg
     1741 atgggagttc acggaggaaa tctccgcatc tggcgagcca cacatctcta tcacgtcatg
     1801 ggtcacctca agcctggcgt cacgcctcag caggccaatt ctgaaatgga tgctctggcc
     1861 cgacgcatct ggaatcagga cacgttgttt accgggtttg gcacctcgtc aattccactg
     1921 cggtcctatc tgtacggcga tcttgggttg cccttgctgg cagtttggct ggcaggcgtt
     1981 ttgattctgc tgattgtgtg tgtcaacgtg gggttgattg tactggcaca tagcctgggc
     2041 cgggaacgcg agttttcgat tcggcaggcc attggcgcct cccgatggcg gttggtacag
     2101 cagtttttga ccgaatcggt atttctctca ttgcttggcg ggttggctgg gcttgggctg
     2161 gcactgctga gtttggaaac cgtgcaccat gtgctggcac catttttccc tgattttcag
     2221 aagctcacgt ttgaccaaac ggtgtggctc caaactgcag gcttaatttt tctggttgga
     2281 gtgggacttg ggtgcagtcc agccgtgcga ctggtcaaat caaatctgaa tttccacctc
     2341 cgacaatcac gcggtcaggg acagagtcac ggacgtctga tctcaggtcg aaatactttg
     2401 attgtggttg aaatcgcggt ctcactggtg ctgctggcgg ggggcgtttt agtcagccgc
     2461 agtctgatca atctgattca agttgatcct ggcattcagg ccgaaaaccg tcttgtgttt
     2521 cgaatcaatt taccgccagc caaatatgct gaggattcac tcaaagtcag tttattgagc
     2581 cggtttgaag ctgatttaaa aaaattaccg ggggtaaaag cggttggcat gggatcaaat
     2641 tatccttttt ccccaactgc ggtgatgccg gcaacgtcaa tttttatcga aggccgcgat
     2701 aaccgcgata tgaacaatgt gccgctggct gatacgcgga ccatcaatgc ggggtatttt
     2761 tctgcgcttg gaatcaatct ggtggctggc cgtgacctga atgagcgtga tactgaaggc
     2821 acccagccgg tcgccatcat caatgagatg atggctcgcc gcttctggcc agggcaaaat
     2881 ccacttgggg cacggcttcg aagcggtgat agcccaaatc cagacgcggt ttacaccgtg
     2941 gtgggcgtgg tcgaagacgt caagaatcgc ggcctgcgga acgaaacgct ccaggaagtc
     3001 tatttttctt atcggcaacg cccaccccgc agcatggctt ttgtggtttc ggggcacatt
     3061 gatccactca cgttcgccgg cacaattcga agtcaactcg cgcagcttga cccaggtctg
     3121 gcgctttttg aaatggcttc gctcggcgaa ctggttcgcc agtccctgat ggtccccaaa
     3181 ttaaccgcct gggttttagg aagtattgcg ggggttgcat ttttcctttc cctgattggc
     3241 atgtttgggg tgattacctt ttccgtcaaa caacgaacct ttgaatttgg cctccgcctg
     3301 gcactgggcg cgcaccccaa tcaaattgtg ggaatggttg tccgccaggg actgtggctc
     3361 gccgtgctcg gattggtggc ggggattttt ctggcgtctt ttgccttgca gatactcaac
     3421 ccattgctct ttgggattga gacttttgat gtgccaacgt tggtgtgtat ttcagtggca
     3481 atcgtaggac ttaccacgtt ggccagttac ctcccagccc ggcatgcggc tgcaatcaat
     3541 ccacttattg ctcttcgatc agaataagga atcaacgtat gaactattca aagcttttgt
     3601 gtgggtcagt gtgtactggt attttggtca gtctgctcag ttgtacgctt tatcctcagt
     3661 tccaatctgc cagtgctaaa gcccagcctc aatcacagaa tgtcagtcga gccactatca
     3721 aagaggcgca ggcacttttt gcgaaacagg aatgggccaa aaccgcggcc atgtgtgaag
     3781 aactggtcaa gaccaatccc tacaatggag caatttggga catgcttgga tttgctcatc
     3841 atcaactcaa gcattttgat caggccatcc ccgcatttca aaaagcggct gaatgcggca
     3901 ccaacccggc ttctgaactc tataacattg cgtgcggata tgcgttgaag ggcgataagg
     3961 aacaggctct ggtctggctc gaaaaagcaa tgaaagctcg gttttccgat tttgaaacga
     4021 ttcgcaatga ctctgagatt gacagcctgc gcagtgaccc gcgctttaaa aagctggttg
     4081 gcgaaaagcc ggatggcacg ctgagccgcg atgaacaatg gcgctatgac ctgcagtatc
     4141 tggcaagaga gttgaaacgg ttgcattaca atctgtacaa caaactggac caggcccgct
     4201 ttgaagccat cgtagctgaa attgatcgca aaatcccaac cctgaaagat catcaaatta
     4261 tcgttgagct ggtatgtctg gctgcggcaa ttggtgatgg acacactttg tttatgccac
     4321 ccacgagcgg tgagggcggt tttcacaaag tcccggttca attttctgaa tttaaagagg
     4381 ggatttttgt cacggcggcg gctgacgaat ataaagcggc ccttggaatg aagttgagca
     4441 agattgggaa gactccggtt gagcaggcat accagatgat ttccaaaacg gtttcccgcg
     4501 ataacgacat ctggttgcga atgcaagttc cccgacggtt tggtgttcct gagtacctcc
     4561 acgccttcgg gatgattcca tcacttgatg aagtcagcta tgttctgacc gatgcccagg
     4621 ggaaagaact gaccatctcg ccaaaaccac gaccatttct ggaacaagtc aacctgatct
     4681 ctgtccgtcc gagtggatct gatgtgacgc cgctgtattt gaaagaccag gaaaatttgt
     4741 actggtacga atacctgaaa gactcaaaaa cggtgttttt tcaatacaat ggggttcaaa
     4801 acaaacctga ggaatcaatt gccgcctttg tgaaaaaaat gtttgccttt atcaatgcca
     4861 atgaggtgga acggctcatt attgaccttc ggtggaatgg cggcggcaat aacttcctca
     4921 ataagccgct ggttcaggaa atcatcaaat gtgaaaagat caatcaaccg ggcaagctgt
     4981 ttgtgattat ggggcgcaac actttttctg cggccatttg cggtgcctcg gatttgcaac
     5041 aacaaaccca ggcgatgttt gttggtgagc catcggcctc gccgccgaac ttcattggcg
     5101 agtccacgat gaccacgctg ccattctgca aattacgggc cagcatttca tcgctgtact
     5161 ggcaaaacgc accggcgatg gattaccggg tgtggattgc tccggatctg gctgccgagc
     5221 caacgtacga ggcttttgtt gccaatcgcg acctggcact cgaagccatc ttcaactatg
     5281 ggaagtagct ttttagggct gaagacattg ggctgagggc ttggggctga aaaaaacagg
     5341 gctgagggct gaagactcgc aagctcgggg ctgaagaaaa cggatttaat accctgagcc
     5401 tgttttcttc agcccgcctt cttcagcccg ttttcttcag ccccaagccc tggttttttc
     5461 agcccaagcc ctggtttttt cagccccaag ccctggtttt ttcagcccga cgtctt
//
LOCUS       DAOQDO010000081         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9243_length_5512_cov_2.892248, whole genome shotgun sequence.
ACCESSION   DAOQDO010000081 DAOQDO010000000
VERSION     DAOQDO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9243_length_5512_cov_2.892248"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..811
                     /locus_tag="PKZ53_02150"
     CDS             137..811
                     /locus_tag="PKZ53_02150"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39263.1"
                     /translation="MNAVLVPPIFKLDGLDQRIVISGVSRETHQRLLADLGDQPVPRV
                     TYWEGVLQLMSPTSQHEQLIELLCEVVKIHARQHKQRIVGTRSMTLEKKGVDGSAQPD
                     GSFYIAHAAQVARTIKIDLTTDPPPDLVIEIDITSSSAQKIQTDGLYSQFGVPEIWRY
                     DLRSGFEMYCLQERRYEVISGSENLPGLLSEMICQVIAKFESEGQLAALEWFESTFES
                     TGLGQN"
     gene            complement(808..1365)
                     /locus_tag="PKZ53_02155"
     CDS             complement(808..1365)
                     /locus_tag="PKZ53_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39264.1"
                     /translation="MALRIEDKWLRYALKETVGTPITKEKLAEIKHLDWSEYRAEAGM
                     DWGTIQDIGPLRHCTNLKVLSFDGNSVSDLTPLSHLANLEEIWLVQNVVSDLSPLKNL
                     HKLKKLVVDMNYYLTSIQPLAGLQGLTYLNIGGTQVSDLTPLRDLPGLKKVTLYGIKG
                     LEVSPESENQKVLDELRQAGVEILI"
     gene            complement(1417..4113)
                     /locus_tag="PKZ53_02160"
     CDS             complement(1417..4113)
                     /locus_tag="PKZ53_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNJ39265.1"
                     /translation="MRQRLPQAQVEWSALTGNPHHVFSYVGALTAPNPTQSPETIART
                     FLRLTPELYQSPSVDLQPETLHLVRSYQTRRTGVTHLTFQQYAGGIPILGGAIRANVL
                     PDGRLLSLSGDLFQLKTINSDLLKTLKQQPGTWNPEPGTRIFQTALESLNLTDENQLR
                     APLKASQVLLPIGKDTLRLAWQVEVQPIDSADHWLVLIDAERHQVLRTYNLTWYAAQT
                     PTPQGRVFATTPQPNLPRVTPPPPGLVEREMRPFAGDPVASPDGWIDLTQRTTVGNNV
                     EAQEDRDANNTGGFRPVLSDEMLFDFPLVLQNPTQGPEQFQAAAVTNAFYWANVMHDY
                     LYALGFDEAAGNFQRDNFGRGGVGNDPVRVDAQDGARINNSSFATSPDGAPGQMSLYF
                     WTRAVPSLDSSLDAEIILHEYTHGLTNRLVGGPQDVTALNPVQSSGMGEGWSDWFALS
                     ILTRAEDPVDGAFPFGSYVAQNFERGLRRFAYSTNLQINPLTFADIDPAQTRFPDDPT
                     QVHKVGEVWCSALWEVRANFIGRYGFEAGRRELEHLVVEGLKLTPTAPTMLDARDAIL
                     LADRIANQGQNQDIIWRGFAKRGMGFTARAFGGGLMTVRQAFDLPPFADPTGRIVLND
                     VIFADGEPLQISVGKASFAQTESVMVEVRSTAGDVETLVLDQADAIPALFEGVLPVKI
                     GQPVTASDGILQAQIGNDLTVVLVNPPDGQPLSTTAKAGQRIAVFTDALESGIGRWKP
                     KNTWALTTISANSATHSWTDSPQGNYPAKANLILTAPKFDLTQVRGAKLTFWHQFDLE
                     RGFDFGFVEAKAGAGTPWQILAVFTGNQLSFQPVTIDLSQFDGKKPKVRFRLTSDTIN
                     QRDGWYIDDVKVLGGSDGAKKPEPEAKPDPETIEIPSDTPNK"
     gene            4258..5217
                     /locus_tag="PKZ53_02165"
     CDS             4258..5217
                     /locus_tag="PKZ53_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNJ39266.1"
                     /translation="METSSQTYIADGLLGHNSKRTFLVPTKYGRSIQLTNLEWDHQAL
                     HEVRHRVGDRTQSVRKTDPALAGVFKGMSSLITPIQLSDEDRRLYAMVREQARHALEN
                     EEGGLAQYAQLLRHICNNPEALHRTDIPLGGQLAAQYPKLATSAHCAKLEVLVAQLEG
                     IRDEGDKAVVFTSWVNTSLLLISKELTKRKINHVIHHGGQDRPVAQAAQDEFKRNPEV
                     TVFLSSDAGAFGLNLPEARFVISYEPTFSWDTMMQRSERINRADSWLDGLTAYTYVTD
                     DSIEERIAAVCDERRQLASVTLGTMETLNTTHNENNPAWLIFG"
     gene            5225..>5512
                     /locus_tag="PKZ53_02170"
     CDS             5225..>5512
                     /locus_tag="PKZ53_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39267.1"
                     /translation="MTVLPFPMPNSDDNPRARNTDPDTSHIGGKRGPNWEAIEHKMLR
                     IYTKALIEERLLADDEVMDECGWGMEKDGHRRRCSDLRKPRMVKGVMVRPPL"
BASE COUNT         1220 a         1532 c         1526 g         1234 t
ORIGIN      
        1 ttgccggcag gatgccggcg ttcccaggcg gaactctttt tctgaagagc tatagaaact
       61 cagggattat ttatctaaaa aactatatct gtgtaaaatt ggaccggtcc tcttttttct
      121 tcgaaatgag gtgtttatga atgctgtttt agtaccgcca atttttaagc ttgatggtct
      181 tgatcagcga atcgttatct ctggagtttc gcgtgaaacc catcaacgtc tcttggcaga
      241 tctgggagat cagcctgtgc ccagggtgac ctactgggaa ggggtgttac agcttatgag
      301 cccaacttca cagcacgaac aattgattga attgttatgt gaggttgtca aaatacatgc
      361 ccggcagcac aagcaacgga tcgtcggcac ccgatcaatg acgcttgaga aaaaaggggt
      421 tgacggttcg gcccaacctg acgggtcatt ttatattgcc cacgcggctc aggtggctcg
      481 gaccatcaaa attgacctga ctaccgatcc tccgcccgat ctggtcattg aaattgatat
      541 caccagtagc tcagcccaga aaattcaaac tgatggtctg tatagtcagt ttggtgttcc
      601 cgaaatctgg cgctacgacc tccgttcagg atttgaaatg tattgtttgc aggaacgccg
      661 gtatgaggtg atttcaggca gcgaaaatct gccagggctt ctatctgaaa tgatttgtca
      721 ggtgattgcg aagtttgaaa gtgaaggtca actggctgca ttggaatggt ttgagtcaac
      781 gtttgaaagt actggcttgg gtcagaatta aatcaagatc tcaactccag cctgtcgaag
      841 ttcgtcgagc accttctgat tttccgattc cggagacacc tccagccctt ttatgccata
      901 aagcgtgact tttttaaggc caggaaggtc ccgaagcggc gtcaagtctg acacctgcgt
      961 tccaccgatg ttcagatagg tcagcccctg taacccggcc agcggttgaa tggatgtgag
     1021 gtagtaattc atatccacca ccagtttttt cagtttgtgg agattcttga gcgggctgag
     1081 atcgctcacc acattttgta ccagccagat ttcttcgagg ttggcaaggt ggcttaatgg
     1141 ggtcaggtcg ctaactgaat ttccatcaaa tgacaggact ttgagattgg tacagtgcct
     1201 gagtggcccg atatcctgaa tggttcccca atccatcccg gcttcggccc gatattcact
     1261 ccagtcaaga tgctttattt cagccagttt ttctttggta atcggcgtgc caaccgtttc
     1321 tttcaaggca tatcgaagcc atttatcttc aattctcaat gccataacct gattgctcct
     1381 caaaggctgg gtgttctgtc ttctggcagg tcagaattac ttgttcgggg tgtcgcttgg
     1441 aatttcaatt gtttccggat ctggcttagc ttcgggttcc ggctttttgg caccgtcact
     1501 gccgcccagc actttcacat catcaatgta ccacccatcc cgctgattga tcgtgtcgct
     1561 ggtgagtcga aaccgaacct ttggtttttt gccgtcaaac tggctcaggt caattgtaac
     1621 cggttggaat gaaagctgat ttccggtaaa caccgccaga atttgccagg gcgttccagc
     1681 cccagctttt gcctcgacaa agccaaaatc aaaacccctt tccaaatcaa attgatgcca
     1741 gaaggtcagt ttggcacccc gaacctgggt caagtcaaac tttggtgcgg tcaagatcaa
     1801 atttgctttg gccggataat ttccttgcgg gctatcggtc caggaatggg tcgcactgtt
     1861 ggctgaaatc gtggtcagcg cccaggtatt tttgggcttc cagcgaccaa tgccggattc
     1921 gagcgcgtca gtaaaaaccg caatccgttg accggccttc gccgtggtcg aaagtggttg
     1981 accgtctggc ggattgacca gcaccacggt caggtcattg ccgatttgtg cctgcaggat
     2041 gccatcactg gcagtcactg gctgaccgat tttgactggg agcactcctt caaacagcgc
     2101 cggaatggca tcggcctgat cgaggaccag cgtttcgaca tccccagccg tgctgcgaac
     2161 ttcaaccatg accgattcgg tttgggcaaa agacgctttg ccaaccgaaa tctgcagtgg
     2221 ttcaccatcg gcaaaaatca cgtcgttgag cacaatgcgc ccggttggat ctgcaaacgg
     2281 tggaaggtca aaggcttgcc ggactgtcat tagtccaccg ccaaatgccc gcgccgtaaa
     2341 ccccatgccg cgtttggcaa aaccacgcca gatgatgtcc tgattttgac cctggttcgc
     2401 aatacggtcc gcgagcaaaa tggcgtcacg cgcatcaagc atcgtcggag ccgtgggcgt
     2461 cagttttaac ccttcaacca caaggtgttc cagttcgcgg cggccagctt caaatccata
     2521 tcgcccgatg aaattggcgc gaacctccca cagcgccgag caccagactt caccgacttt
     2581 gtgaacctgg gttgggtcgt ctgggaaacg ggtctgcgcc gggtcaatat cggcaaacgt
     2641 caggggattg atttgcaagt ttgtgctgta ggcaaagcgt cgcaatccgc gctcaaagtt
     2701 ttgtgccaca tagcttccaa aaggaaacgc cccatcaacc ggatcttcgg ctctggtcag
     2761 aatcgagagt gcaaaccagt cagaccagcc ttcgcccatg ccagagcttt gcaccgggtt
     2821 gagtgcggtc acatcctgcg gaccacccac gagtcggttg gtcaagccgt gggtgtattc
     2881 gtggagaata atttcggcat ccagcgatga atcaagcgac ggcacggctc gcgtccaaaa
     2941 atacaggctc atctggcccg gagcaccatc tggcgaagtg gcaaaactgg aattattgat
     3001 ccgtgcgccg tcctgggcat caacccgcac cggatcattc cccacgccgc cacgcccaaa
     3061 gttgtcacgc tggaaattcc cggcagcttc gtcaaaccca agcgcataca gataatcgtg
     3121 catgacattg gcccagtaga aggcattggt cacggcggcg gcttgaaact gttctggacc
     3181 ctgagttgga ttttgcagaa caagcggaaa atcaaagagc atctcatctg acagaaccgg
     3241 tcgaaagccg ccagtgttgt tggcgtcacg gtcttcctgc gcttcgacat tgttgccgac
     3301 cgttgtgcgc tgcgtcagat caatccagcc atctggggag gcaaccgggt caccggcaaa
     3361 cggacgcatc tcgcgctcaa ccagccccgg cggcggcggg gtcacccgtg gcaagttcgg
     3421 ctgtggtgtg gtggcaaaca cccggccctg tggtgttggt gtctgagcgg cataccacgt
     3481 caggttataa gttcgcagaa cctgatggcg ttcggcatct atcagcacca gccagtggtc
     3541 agcactgtca atcggttgaa cttccacctg ccaggccagc cggagcgtgt cttttccaat
     3601 cggcaacagc acctgactgg ctttgagtgg tgctcgaagc tggttttcgt cggtgaggtt
     3661 gagggattcg agagccgtct ggaaaatccg ggttccgggt tccgggttcc aggttccggg
     3721 ttgttgtttc aaggttttga gtaagtctga attgatcgtc ttgagctgaa acaggtcgcc
     3781 ggagagggaa agcaacctcc catcgggcaa cacattggcc cgaatcgcgc cgccgaggat
     3841 gggaatgcca ccggcatatt gctgaaaggt caggtgtgtg acgccggtgc gccgggtctg
     3901 atagcttctg accaggtgga gtgtttctgg ttgcaggtca actgacgggc tttgatacaa
     3961 ctctggcgtc aggcgcagaa aggttcgggc aatggtttca ggcgattgcg tggggtttgg
     4021 tgccgttaac gctccgacgt aggaaaacac gtgatgtggg tttccagtca atgcactcca
     4081 ttcaacctga gcctgaggca gccgctggcg caatttttga atgcgtgctc ttcgctcggt
     4141 ttgctcagag actgaaaccc caagttgagc aaggtctggg aaggaaagag tctccgtgcc
     4201 tgtggccgag caatcgtcaa atccgttacc cagcttccgc taggcgaaat tgttgcaatg
     4261 gagacaagca gtcagaccta cattgccgat ggtctactgg ggcacaactc caagcgcacc
     4321 ttcctggtgc cgaccaagta cgggcgctct atccagttga ccaacctgga atgggaccac
     4381 caggcgctgc atgaggtccg gcaccgggtg ggggatcgca cccagtcggt gcgcaagacc
     4441 gaccccgccc tggctggggt gttcaagggc atgtcctcgc tcatcacccc catccaactg
     4501 tcggatgagg accgccgcct ctacgccatg gtcagggagc aggcccgcca cgccttggag
     4561 aacgaggaag gtgggctggc gcagtacgcc cagctgctgc ggcacatctg caacaacccc
     4621 gaggcgctac accgcacgga catcccgcta ggcgggcagc tggcggctca gtaccccaag
     4681 ctcgctacgt cggcgcactg cgccaagctg gaagtcctgg tggcccaact agagggcatc
     4741 cgcgacgagg gcgacaaggc agtggtgttc accagttggg tcaacaccag cctgctgctc
     4801 atctccaagg aactgaccaa gcgcaagatc aaccacgtca tccatcacgg cgggcaggac
     4861 cgtccggtgg cccaggctgc ccaggacgag ttcaaacgca accccgaggt gacggtgttc
     4921 ctcagttcgg acgccggggc gttcggcctc aacctgcccg aggcccggtt cgtcatctcc
     4981 tacgagccca ccttcagctg ggacaccatg atgcagcgca gcgagcggat caaccgggcc
     5041 gactcctggc tcgacgggct gacggcctac acctacgtca ccgacgactc cattgaggag
     5101 cgcatcgcgg cggtctgcga cgaacgccgt cagctggcct cagtcacgct gggcaccatg
     5161 gaaaccctca acaccaccca caacgagaac aaccccgctt ggctgatctt cggataggag
     5221 aaccatgacc gtcctgccct tcccgatgcc gaactctgac gacaaccctc gcgcccgcaa
     5281 caccgatccc gacaccagcc acattggcgg caagcgtgga cccaactggg aagccatcga
     5341 acacaagatg ctgcgcatct acaccaaggc actaattgag gagcgcctgc tggccgacga
     5401 tgaggttatg gacgagtgtg ggtgggggat ggagaaagac ggccatcgac gccgctgtag
     5461 cgacctgcgc aagcccagga tggtcaaggg cgtcatggtc cggcccccgc tg
//
LOCUS       DAOQDO010000082         5491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9304_length_5491_cov_2.959345, whole genome shotgun sequence.
ACCESSION   DAOQDO010000082 DAOQDO010000000
VERSION     DAOQDO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9304_length_5491_cov_2.959345"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKZ53_02175"
     CDS             <1..744
                     /locus_tag="PKZ53_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39268.1"
                     /translation="GTGKTFLAEAFAKDCGLNVVEFKNFRDKWVGSTESNLEKILNLI
                     QNLAPIVVLIDEADATLGNRDSGGGDSGVDQRIFSKVANAMGNTENRGKILWILMTCR
                     PDLLPIDLKRQGRCEEHISLFYPESEEDRQAIIEAMLRKNKISHKVEDWSPIQKSQLK
                     LSGADIEGMLIRCRRVARQAGRKEVLPEDVEKVALEFTPARDEMAVEYQTLVAVREAT
                     SRDMLPEAYRHLSPAEISQRIEELRMLTR"
     gene            797..3643
                     /locus_tag="PKZ53_02180"
     CDS             797..3643
                     /locus_tag="PKZ53_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39269.1"
                     /translation="MQTSFDVLDACPVPILVLKQGMVQFVNREALAILGTTDCQEVLG
                     MECVDFVHPEDLQKVQTWLASTLQSTSRLRARLLRMDGQPIQAEMKASSFEDQSELAV
                     QVAIVSSSMNKIAVTDAGDIYRELYENANDLIYTHDMEGHFTSINRAVERVFGIPREA
                     VLQMNMRDVVSPEYLTTVHKMMEQKVAENIPTRYEIEIVTPAGVRIPIEVSSRIITLN
                     GRPVGVQGIARDITERKRAEALQKIAHDAALESVRLKSQFLANMSHEIRTPLNGVIGM
                     THLLLGTDLTAKQMDFVETIQNSGETLLKIINEILDLSKIEAGKMTLENIELNLEVLV
                     DQVITMFAERAHSKGLELVTHIQKNVPKTLLGDPVRIRQIITNLVANGIKFTDKGLVT
                     LQISLLHEENDTATVQFKVTDTGIGIPVDLQSRLFRPFSQADGSTTRRYGGTGLGLAI
                     CKQLTELMGGEIDVESYPGQGSTFRFSIPLARAHKKGEDARQSDPDGLKKLRVLLVDN
                     NAANRDLLQQQLTEWEMTTDVAETGLQALEHLRNAVHRAKPFDLVIEDMLLPGMDGME
                     LARAIKSDPLIAQTKLVMLTSFAMRGHGDEARQSGIAAYLTKPVRQTHLRNCLLQVMH
                     RTDHAGPQPLITRYTLASSSSLLSVPQLPQMRVLLVEDIEANQVVARLILENLHCRVD
                     VASNGLKALGMLAENSYDLVFMDCQMPLMDGFETTAVIREQEEQSGKHIIIVALTAGA
                     MEGDRERCLQVGMDDYISKPFRPEDFERALQRWVVGAESAVPAPVRTTASLSSESVAS
                     PVVDEIEEDVEDDDTPTLTPESGSGLMPVTNHSISPPVLDESTVTQLLELTRQDTGVL
                     YRLMMMSFDTLVPFPDALQKAFARADFEHIGKIAHKLRGTGGSIGAIGIIRIGQALEE
                     IKSSAALADAPFLVAEFRAELTRLKEEVNRRFHP"
     gene            4016..5428
                     /locus_tag="PKZ53_02185"
     CDS             4016..5428
                     /locus_tag="PKZ53_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39270.1"
                     /translation="MSTHYPDDKTVITQLTKGSILIVDDNPTNLELLSNLLMERQYEV
                     KAAINGRRALAQVNLSHPDLIMLDINMPGMDGYEVCRRLKEDESTSRIPIIFISALNE
                     VWDKVKAFEVGGIDYVTKPFQCEEVIARIETQMKLSQLQREMERKNAELTQKNQELLM
                     AYQRVRTLFSTLAEVLPGTVLDNKYRLDTKIGSGGFGAVFQATHLNLNRDVAVKVFQP
                     PTDENRFEELERFRMEGISACRVNHPNAITVLDSGVSPEGIAYLVMELLSGHTLFQEL
                     KQKVRLPLERCAEIIFPICDVLDAAQMAGIVHRDIKPENIFLHQSPDGEVVKVVDFGI
                     AKLLGDPVNPNLPSLTLNGVVGTPAYMSPERFNRKDYDGQADIYSLGVLFYRMLTGSL
                     PFDAADPVALALMHLTKSPQPPRQRNPEIPQAIETIILQMLSKTPGVRPTPRQLALSL
                     QTALERARFDSSDLGEDLYY"
BASE COUNT         1398 a         1343 c         1455 g         1295 t
ORIGIN      
        1 ggaacgggca agaccttcct ggccgaagct tttgccaagg attgtggcct caacgtggtg
       61 gaattcaaaa acttccgcga taaatgggtt ggatcaacgg aatcaaacct cgaaaaaatt
      121 ctgaatctga tccaaaacct ggccccgatt gtggtgctga ttgacgaagc cgacgccacg
      181 ctcggcaatc gtgattcggg tggcggagac agcggcgtgg accagcggat tttctcgaaa
      241 gtcgccaatg cgatgggcaa taccgaaaac cggggcaaaa ttctgtggat tttgatgacc
      301 tgtcggccag acttgctccc gattgacctc aagcgccagg gccggtgcga agagcatatt
      361 tcgctgtttt atccggaatc agaggaagat cgtcaggcga ttattgaggc gatgctccgc
      421 aaaaacaaaa tcagtcataa agttgaagac tggtcgccaa tccaaaagag ccagctcaaa
      481 ctctctggcg ctgacatcga agggatgctc attcgctgcc gccgtgtggc ccggcaggct
      541 ggtcggaaag aagtcctgcc cgaagatgtt gaaaaggtcg cgctcgaatt caccccagcc
      601 cgcgatgaaa tggcggttga atatcaaacc ctggtggctg tgcgtgaagc gacatcacgg
      661 gatatgcttc cagaagccta tcgccacctc tcacctgctg aaatcagcca gcggattgaa
      721 gaactgcgca tgttgacccg atgacctgtg gcgtgcggcc cacctgacgc cgctttcaac
      781 tgacacacct atgccaatgc aaacctcttt tgatgtgctt gatgcctgtc cagtgccaat
      841 cctggtgctg aaacaaggaa tggtccaatt tgtgaatcgc gaggcattgg caattcttgg
      901 aacgactgac tgtcaggaag tgctgggaat ggaatgcgtt gactttgttc acccagagga
      961 tttgcagaaa gtccagacgt ggcttgcttc aacccttcaa tcaacatcgc ggctccgagc
     1021 gcgattgctt cggatggatg gccaacccat ccaggccgaa atgaaagcgt caagctttga
     1081 agatcaatcc gaactggcgg tccaggtggc gattgtctct tcgagcatga ataaaattgc
     1141 ggtcacggat gccggagaca tttatcgcga gctgtatgaa aatgccaatg atctgattta
     1201 tacccacgat atggagggac attttacttc catcaatcga gccgttgagc gggtgtttgg
     1261 aattccacgc gaagccgtgc tgcaaatgaa tatgcgggac gttgtttcac cagagtacct
     1321 cacgaccgtt cataagatga tggagcagaa agtggcggaa aacattccca cccgctacga
     1381 aatcgaaatc gtaacgccgg ctggagttcg aatcccgatt gaagtgagtt cgcggatcat
     1441 caccctcaat ggtcgtccgg ttggtgtcca ggggattgca cgcgatatta ccgaacgcaa
     1501 acgcgctgaa gcgctccaaa aaattgccca cgatgcggcg cttgaatccg tccgcctgaa
     1561 atcacaattt ctggccaata tgagccacga aatccgcacg ccgctcaatg gcgtgattgg
     1621 aatgactcat ctgttactgg ggactgacct gaccgccaaa cagatggatt ttgttgaaac
     1681 cattcagaac tcaggcgaga cgctgctcaa aattattaac gagattctgg atctttccaa
     1741 aatcgaagct ggcaagatga cgctcgaaaa catcgaactc aacctggaag ttctggtgga
     1801 ccaggtcatc acgatgtttg ccgaacgcgc ccacagcaag ggcctggagc tggtgaccca
     1861 tattcagaaa aacgtgccaa agaccttgct tggtgatccg gttcgcattc gacaaatcat
     1921 caccaatctg gtggccaatg ggatcaagtt tactgataag gggctggtga cgctccagat
     1981 tagtctgctc cacgaagaaa atgacacggc aaccgtgcaa ttcaaggtga ctgataccgg
     2041 gattggcatt ccggttgatt tgcaaagtcg gctatttcgc cccttttcac aggctgacgg
     2101 atcaacgacc cgccgatatg gtggcaccgg actggggctg gcgatttgta agcagttgac
     2161 cgagttgatg gggggcgaga ttgacgttga aagttatcca gggcagggca gtacttttcg
     2221 attttcaatt ccgctggccc gggcacacaa gaaaggagaa gacgctcgtc agagtgatcc
     2281 tgatgggctc aaaaagctgc gggtcctgct cgtagataac aatgccgcca atcgtgactt
     2341 gctgcagcag caactcaccg aatgggaaat gaccactgac gtggctgaaa ctggcctgca
     2401 ggcattggaa caccttcgga acgcggtgca tcgtgccaaa ccatttgatc tggtcattga
     2461 ggacatgctg ttacctggaa tggatgggat ggagctggcc agggccatta aatctgaccc
     2521 cctcattgcc caaaccaaac tggtgatgct gacatcgttt gccatgcgtg ggcacggtga
     2581 tgaagcccgc cagtcaggga ttgcggccta tctgaccaag ccggttcgac aaactcatct
     2641 gcgcaattgc ctgctccagg tgatgcaccg aactgaccat gccggtccac aaccactcat
     2701 tacccggtac acgcttgcct caagtagttc gctgctctct gttccacaac taccgcaaat
     2761 gcgggtgctg ctggtcgagg atatcgaagc caaccaggtc gttgcccggc tcattctgga
     2821 aaatctgcat tgccgggtgg atgtggcgtc caatggtttg aaagcactgg ggatgctggc
     2881 ggaaaacagc tacgacctgg tgtttatgga ttgccagatg ccgctcatgg atggatttga
     2941 aaccacggct gtaattcgcg aacaggaaga acaaagcgga aaacacatca tcattgtggc
     3001 cttgaccgcc ggggcgatgg aaggtgaccg tgagcggtgc cttcaggtgg gaatggacga
     3061 ttatatcagc aagccgttcc ggcctgagga ttttgaacga gccctgcaac ggtgggtggt
     3121 gggcgctgag tcggcggttc cagctccggt caggaccact gccagtttgt catccgaatc
     3181 agtcgcgtcc cccgtggttg acgaaatcga agaagacgtg gaagacgatg atactccaac
     3241 cttgacacct gaaagcggtt cggggttgat gcctgtgacc aatcattcca tctcaccacc
     3301 tgttttggat gaaagcactg tgacccagct tctggaatta acccgtcagg atacaggtgt
     3361 tttgtaccgg ttaatgatga tgtcttttga taccctggtg ccatttccag acgcgctcca
     3421 gaaagccttt gcccgggctg actttgaaca tatcggaaaa attgcgcaca agctccgtgg
     3481 gacaggtggc agtatcggcg ccattgggat tatccgcatc ggtcaggcat tggaagaaat
     3541 caaatcttcg gcagctctgg cggatgcccc atttctggta gcagagtttc gggctgaact
     3601 gacccgtttg aaggaagagg tcaaccgacg gttccatcct tagaatgaag aatgaagaat
     3661 gaagaatgaa gaattgagaa ttgagaattg agaatgaaga atgaagaatg aagaatgaag
     3721 aatgaagaat tgagaatgaa gaaaaaatag tttttgattc ttcttcgaaa gtattgatat
     3781 ttttttaagg gttatctcaa aactcaagga tagatcagtt ctattctttc aggcaggtga
     3841 ccgtgactat gtagccttga tgagtgcgcc attcggactg tcttctgaaa tcaaagctga
     3901 gttttggtga taggactgaa acacattctc aattcttcat tctcaattct tcattctcaa
     3961 ttctcaattc ttcattctca attctcaatt cttcattctt cagcgcaggt ttgcaatgag
     4021 cacacattac ccagatgaca aaaccgtaat cacacaactg accaaaggga gcatcttgat
     4081 tgttgacgat aacccgacca atctggagct gctttcaaac ctgttgatgg aacgccagta
     4141 tgaggtcaaa gcggcgatta atggtcgtcg agcgttggcg caggtcaatt tgtcgcaccc
     4201 ggacctgatt atgctcgaca ttaacatgcc ggggatggat ggctatgagg tctgccgccg
     4261 actcaaagaa gacgaatcca ccagtcgaat tccaattatt ttcatcagtg ccttaaatga
     4321 ggtctgggat aaggtaaaag cgtttgaagt tggcgggatt gactatgtga ccaagccgtt
     4381 tcaatgcgaa gaagttattg cccggattga aacccagatg aagctgtcgc aattgcagcg
     4441 ggaaatggaa cgcaaaaatg ccgagctgac ccaaaaaaac caggaattgc tcatggcata
     4501 ccagcgagtc cggacacttt tttcaacgct ggccgaggtt ttgcctggta cggttctgga
     4561 caacaaatat cgccttgata ccaaaatcgg ctctggtggg tttggggctg tatttcaggc
     4621 cacgcacctc aatctcaatc gggatgtggc tgtcaaagtt tttcagccgc cgacggatga
     4681 aaatcgcttt gaggaactcg aacggtttcg catggaaggg atttccgcct gtcgagtcaa
     4741 tcatccaaat gccataacgg tgcttgactc aggtgtctca cctgaaggca ttgcctacct
     4801 ggtcatggaa ttgctctctg gccacacgct gtttcaagaa ctcaagcaaa aagtcagatt
     4861 gccgctggaa cgatgcgccg agatcatttt tccaatttgt gatgtgctgg atgcggctca
     4921 aatggccggg attgtgcatc gtgacatcaa gcctgaaaac atatttcttc atcagtcacc
     4981 agacggagaa gtcgttaaag tggttgattt tgggattgcc aaactgcttg gcgacccggt
     5041 gaatccaaat ttgccttccc tcacactcaa tggcgtcgtg ggaacacccg cctatatgtc
     5101 gcctgaacga tttaaccgga aagactatga tggccaggcc gatatctaca gtttaggtgt
     5161 tctgttctat cgaatgctga cgggttcgct cccgtttgat gctgccgacc cggttgccct
     5221 ggctctgatg cacctgacga aatcaccaca gccaccacgt caacgaaatc ccgaaattcc
     5281 tcaggcgatt gaaacaatca ttctccaaat gctctcaaag acacccggcg ttcgtccaac
     5341 tccacgccag cttgccctca gtttacaaac ggcacttgag cgagccagat ttgatagctc
     5401 ggatctcggg gaagatttgt actattgaat cgggggcagg ggttcagggt tccgggttcc
     5461 gggttccggg ttccgggttc cgggttccgg g
//
LOCUS       DAOQDO010000083         5487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9315_length_5487_cov_3.425994, whole genome shotgun sequence.
ACCESSION   DAOQDO010000083 DAOQDO010000000
VERSION     DAOQDO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_5487_cov_3.425994"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKZ53_02190"
     CDS             <1..534
                     /locus_tag="PKZ53_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HNJ39271.1"
                     /translation="ADAVMRLRAEYLFASGKKSDIAFHFTSGFRCDFAKWAEGYRPEV
                     TGNNVRWVKTASVDASYKSFREYLIPVFTYAGTASLSQELKPVSTIQEVEIGDVFIQG
                     GFPGHAVIVVDVALHQQTKDRLFLLAQSYMPAQEIHILRNPTNDQLSPWYSANAQGNL
                     ETPEWTFPPGSLKRFGE"
     gene            complement(553..921)
                     /locus_tag="PKZ53_02195"
     CDS             complement(553..921)
                     /locus_tag="PKZ53_02195"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNJ39272.1"
                     /translation="MSGELFPEAVNVNRSTATMELVWRDGEKSVFRLMSLRRVCPCAT
                     CAQERGENPHPSLVEEVPVAPEKKKRSLSLPLLGDVTKFEVTSMHHVGRYALGVTWQD
                     NHSSIFTWAFLRQAGEEGLK"
     gene            1116..1598
                     /gene="bcp"
                     /locus_tag="PKZ53_02200"
     CDS             1116..1598
                     /gene="bcp"
                     /locus_tag="PKZ53_02200"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNJ39273.1"
                     /translation="MSSETPRELKVGDPAPAFSLQTGDGTTVNLSDFKGQRVVLYFYP
                     RDNTPGCTREACGFRDDLAKLNSLRAQVLGVSTDSVASHKKFTEQYQLNFPLLSDPEM
                     TVSQAYGVWQEKSNYGKKYFGIVRTTFIIDEEGTISRIFRRVKVDGHTEKVLEALAAK
                     "
     gene            1847..2356
                     /locus_tag="PKZ53_02205"
     CDS             1847..2356
                     /locus_tag="PKZ53_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015122664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ39274.1"
                     /translation="MEIPTLTTERLLMRAFRESDLDEYAALCADPEVMRYLGEGRPLA
                     RWEAWRQMAMIIGHWQLRGYGMWAVEEKSTGKLLGRIGCFYPEGWPGFEIGWTLGRAA
                     WGKGFATEGARASLDFAFHTLDQNHVISLIRPDNQASIRVAERLGESLEGSVTLFGGE
                     ALIYGIHRS"
     gene            2741..4843
                     /locus_tag="PKZ53_02210"
     CDS             2741..4843
                     /locus_tag="PKZ53_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNJ39275.1"
                     /translation="MTLDDVATPFRFFGDTADVQWLPDGQSYLIENPAGGGLAKVDVA
                     TGRMTPFHDAPRIAQGLKTTGLGDGEANGLASLSNCTFNAGYTAALFDYKNDLFACDL
                     TTGKAIRLTNSPDKSELEASFSPDGQWVAFVRDSNLFVVSVADGKERALTQDGSEKIF
                     NGYLDWVYEEEVYGRGTKRAYWWAPNSSGLAYLRIDDTQVPPFTVIDDLVPAQKIEIT
                     NYPKVGDPNPLVKLGVASVQTGSTQWMDLSAYQPIEFLITRVGWKPDSTSVVFEVQDR
                     IQTWMDLNLGNAQSGKLQKLIHEESKAWVDVTELPLWIPDGTFYWLSDRSGYRHLYHF
                     GADGKLINAVTKGNWDVRDFHGVSADGWAYFSGTEHSSIGNDVYRIQTDGSGLQRLSN
                     QDGTHAASFSPALNAYVDTWSSLTKAPKSQLLSNNGTLIRTLAENKDNPLREYNLGSS
                     ELLKVSTRDGFEMNALMIKPPDFDPTKKYPVLTHTYGGPGSPQVRDSWIGRWTLWHLF
                     MAQKGYIVWICDNRSASNKGVVSAHPVYRNLGELELRDLEDGVSYLKKLPYVDGGRIG
                     IWGWSYGGYMTAYALTHSTTFKVGIAGAPVTDWRNYDSIYTERYMGLPKDNPEGYRKS
                     SVLEAAGNLHGKLLLIHGLIDDNVHPQNSVQLIEALQKAGKQFDFMVYPASRHGVSQP
                     HRVKHLQKLMTRFITENL"
     gene            4864..>5487
                     /locus_tag="PKZ53_02215"
     CDS             4864..>5487
                     /locus_tag="PKZ53_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNJ39276.1"
                     /translation="MSASMTMPSNQAPAKEFFGHPRGLSVLFFTEVWERLSYYGMRAI
                     LVLYMVASVADGGLGYDTKKAAFVYGLYTSSVYLTAIPGGLIADNFLGAKMAVLLGGI
                     IIALGQFSLTLGSEMTFYGGLVLIAIGTGLLKPNISAMVGALYTPEDRRRDSGFSIFY
                     MGINVGAWLAPFACGFFAQSETFKAFLRSSNIAPSTCWRWAFAVAGFG"
BASE COUNT         1338 a         1421 c         1402 g         1326 t
ORIGIN      
        1 gccgatgccg tgatgcggct tcgggcggag tatctttttg catccggaaa aaaatctgac
       61 atcgcctttc attttaccag cggcttccgg tgtgattttg caaagtgggc tgaagggtac
      121 cgacctgaag tcaccggaaa taatgtacgc tgggttaaaa cagcgtcagt tgatgcctct
      181 tacaaaagct ttcgggaata tctgatccca gtttttacct atgcgggaac ggcgtcactc
      241 agtcaggaac tgaaacccgt ttccaccatc caggaagttg aaatcggcga tgtttttatt
      301 caaggaggat ttcccggcca tgcggtgatt gtggtggatg tggccctgca tcaacaaacc
      361 aaagaccgac tgtttttact cgctcaaagc tatatgcccg cccaggaaat ccacatcctg
      421 cgcaacccaa ccaatgatca gctcagcccc tggtacagcg ccaatgccca gggcaatctt
      481 gaaacaccgg agtggacctt cccacccggc agcctgaagc ggtttggtga gtagtcagaa
      541 aatgcgactc gcttacttga gaccttcttc cccagcctgc cggagaaacg cccaggtgaa
      601 aatactggaa tggttatctt gccacgtcac gcccagcgca tatcgcccga cgtggtgcat
      661 cgaagttact tcaaattttg tgacatcgcc gagcagtgga aggctgagcg agcgtttttt
      721 cttttcaggc gcgaccggaa cctcttcaac gaggcttgga tgcgggtttt cgccgcgttc
      781 ctgggcacag gttgcacagg ggcaaacccg ccgcaggctc atcaatcgga atacactctt
      841 ttcgccatcg cgccagacca gttccatggt ggcagttgag cggttgacat tgaccgcttc
      901 ggggaatagt tcacctgaca tagtcggatt cttccttcga ttgtggttgt aattgctgga
      961 aatatggggc tttccccatg gggtgtttgg tgataacata cgcccctctt atcgccaggc
     1021 gcaatccaag caaacggcac tcgttcgaca atccaaaata tcttcccaac gtacgcgacc
     1081 caaatccaat ctcattgtgt aaggaaacgt ctgccatgtc atcagaaacc ccacgcgaat
     1141 tgaaagtggg cgatcccgca ccagccttca gtttacaaac cggcgacgga acaacggtca
     1201 acctgtctga tttcaaaggt cagcgcgtcg tgctctattt ttatccacgc gataacacgc
     1261 caggatgcac ccgcgaagcc tgtggctttc gtgatgacct cgccaaactc aactctctgc
     1321 gcgctcaggt cctgggcgta agcactgaca gtgtggcgtc gcacaaaaaa tttaccgagc
     1381 agtaccagct caactttccg ttgctgtctg accccgaaat gacagtgtca caggcatatg
     1441 gtgtctggca ggaaaaaagc aactatggaa agaaatattt cggcatcgtt cgaacgacct
     1501 tcatcatcga cgaagaaggc accatcagcc ggatcttccg ccgggtcaaa gttgacggtc
     1561 acaccgaaaa agtgctcgaa gcgttagctg ccaaataata ccggtttgta gtcagtagtc
     1621 agtagtttgc tcaatttatt tgattgaatc acttgactat ttgcttatac cgaagatttc
     1681 gttgcaaatc ggcatctgac cattttggaa taaagccctt gtcttacagg aaggctacat
     1741 tctcgaaaaa atggtatttc cctaaaccct gaaccctgaa ccctgaaccc taaatggtat
     1801 aaggccagcc ccttccaacg accaatccca atcaaagctg ttttccatgg aaattccaac
     1861 cctcaccact gagcgcctgt tgatgcgggc ctttcgtgaa tcagaccttg atgagtacgc
     1921 cgccctgtgt gctgacccgg aggttatgcg ctacctgggc gaaggtcgcc cgctggcccg
     1981 ctgggaagcc tggcggcaga tggccatgat catcgggcac tggcagttac gcgggtatgg
     2041 gatgtgggcc gtcgaagaaa aatcaacggg gaaactcctt ggccgcatcg gatgttttta
     2101 ccccgaaggc tggcctggat ttgaaatcgg ctggacgctg ggccgcgccg cctggggtaa
     2161 agggtttgcc accgaaggtg cccgggcgtc gctggacttt gcatttcaca ccctcgatca
     2221 gaatcacgtg attagtttga ttcgccccga caaccaggct tccattcgcg tggccgaacg
     2281 ccttggtgaa tcactcgaag gctcggtcac tctgttcggg ggcgaagcgt tgatttatgg
     2341 gattcatcgt tcctgagaag caggggctga gggctaaggg ttaagggctg aagaaaaacc
     2401 tgaagttatc tgaacctgtt tttcaatctt cagttccagg ccctaaaaag ccagatgcca
     2461 gataacgctc aaaaattcta agaaaaaaac attccaaacc attcaggcca aacatttacc
     2521 caaatactca cttctgacga tctcacttta tctttgtgtt ttctttcact tcggagtcta
     2581 ttcaatgaag gttcgttatt tgtctttgtt gtgcctgtca ctcttttttt caaccacctt
     2641 gggcattgcc caacacgcgg gaactgcctc ccgaaacttg cttaaatccg gaactcggac
     2701 ccctgaacct cgagttcagt caactcagca gaaaaaagag ttgaccctcg acgatgtagc
     2761 caccccattt cgattttttg gggatactgc cgatgtgcaa tggctgccgg atgggcagtc
     2821 atatttgatc gaaaatccag ccggtggcgg attggccaaa gttgatgtgg caacgggtcg
     2881 gatgacaccg tttcacgatg ccccacgaat tgctcagggg ttaaaaacca ccgggctcgg
     2941 tgacggagaa gccaatggcc tggcatcgct gagcaattgc acgttcaatg ccgggtatac
     3001 agcggctctt tttgattaca aaaatgatct ctttgcctgt gatttgacca ctggcaaagc
     3061 cattcggctg acgaactcac cagataaaag cgaactggaa gccagtttca gccctgatgg
     3121 gcagtgggtc gcttttgttc gtgattccaa tctgtttgtg gtttccgtgg ctgacggcaa
     3181 agaacgcgcc ctcactcagg acggaagcga aaaaatcttc aacggctatc tcgattgggt
     3241 gtacgaagag gaagtctatg gtcgcggtac caaacgcgcc tactggtggg cccccaactc
     3301 atcgggtctg gcctacctgc ggattgatga cacccaggtg ccgcccttta cggtgattga
     3361 tgacctggtg ccggcccaaa agatcgaaat caccaactat cccaaagtcg gcgatccaaa
     3421 cccactggtc aaactcggcg tggccagtgt acagactgga agcactcaat ggatggacct
     3481 gtctgcctat cagccgattg aattcctcat cacccgtgtc ggctggaaac ctgattcaac
     3541 cagcgtggtg tttgaagttc aggatcgaat tcaaacctgg atggatctaa acctgggcaa
     3601 tgcgcagtct ggaaaactcc aaaagctgat acatgaagaa agcaaagcct gggtagacgt
     3661 gacggaatta ccgctttgga taccggatgg cactttctac tggctgagcg accgctctgg
     3721 atatcgccac ctctatcact ttggggcgga tggcaaactg atcaatgccg tgaccaaagg
     3781 caactgggat gtgcgcgact ttcatggtgt ttcggctgat ggatgggctt acttttcagg
     3841 tactgaacac tcgtcaattg ggaacgatgt ctatcgcatt caaaccgacg gctcgggact
     3901 gcaacgcctg agcaaccagg acggcacgca tgcggccagc ttcagtccgg cgctgaatgc
     3961 ctatgttgat acctggagca gcctgaccaa agccccgaaa tcacaactct tgagcaataa
     4021 cggaacgctg attcgcacac tggcggaaaa taaagacaac ccgttgcgcg aatataattt
     4081 aggcagcagc gaactgctca aagtttccac tcgtgacggc tttgaaatga atgcactgat
     4141 gatcaagccg cctgatttcg acccgaccaa aaaatacccg gtgctgacgc atacctacgg
     4201 tggtcctggc tcaccacagg ttcgggattc ctggattggc cgctggacgc tctggcatct
     4261 gtttatggcc caaaaaggct atatcgtctg gatttgcgat aaccgatccg ccagcaacaa
     4321 aggtgtggtc tcagcccacc cggtgtaccg gaatctgggc gaactggaat tacgcgattt
     4381 ggaagacggc gtcagctact tgaaaaaact cccctatgtt gacgggggac ggattgggat
     4441 ctggggttgg agttatggtg gctatatgac cgcgtatgcc ctgacgcaca gcactacctt
     4501 taaggttggt attgccggcg ctccggtcac tgactggaga aactacgatt caatttatac
     4561 cgagcggtat atgggacttc ccaaggataa tccagaaggc taccgcaaaa gctcggtgct
     4621 cgaagcggct ggaaaccttc acggcaaact gctgctgatc cacggactga ttgacgacaa
     4681 tgttcacccg caaaacagtg tgcagttgat cgaagcctta caaaaagccg ggaaacaatt
     4741 tgactttatg gtgtacccgg cgtcacggca cggtgtttcc caaccacatc gagtcaaaca
     4801 cctccaaaaa ctgatgacac ggttcatcac agaaaatctt taaggagaaa aaggactgaa
     4861 cttatgagtg cctcaatgac aatgccatcg aatcaagctc cggcaaagga gttttttgga
     4921 catccacggg gcttatccgt gctgtttttt accgaagtgt gggagcgcct cagctactat
     4981 ggcatgcgcg ccattttggt actctacatg gttgcttccg ttgcagacgg aggtcttggt
     5041 tatgacacca agaaagccgc ttttgtctat gggttatata catcatcagt ctatctcaca
     5101 gccatccctg gtgggttgat cgcggataac ttccttggtg cgaaaatggc ggtgttgctc
     5161 ggtggcatca tcattgccct gggtcagttc tcactgacat tggggtctga aatgaccttt
     5221 tatggtggac tcgttttgat tgccattgga accggtttat taaaaccgaa tatcagcgcc
     5281 atggttggag cgctctatac tccagaagat cggcgacgag attccgggtt ttcgattttt
     5341 tacatgggca tcaacgttgg ggcgtggttg gcaccgtttg cctgtggatt ctttgcccaa
     5401 agcgaaacat tcaaagcatt tctgcgcagt tcaaatattg ctccgagcac ctgctggcga
     5461 tgggcctttg cggtggccgg atttggt
//
LOCUS       DAOQDO010000084         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9438_length_5448_cov_2.715001, whole genome shotgun sequence.
ACCESSION   DAOQDO010000084 DAOQDO010000000
VERSION     DAOQDO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9438_length_5448_cov_2.715001"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1751)
                     /gene="lnt"
                     /locus_tag="PKZ53_02220"
     CDS             complement(66..1751)
                     /gene="lnt"
                     /locus_tag="PKZ53_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNJ39277.1"
                     /translation="MSSKPSVYVRLAQLQPYVLTGVLLGLPALAFSGIRFEFLAWFGL
                     VPMLVALRSETSVRRWFGLSFVALSTWFAVGQSWILLTSPTVATVSFFVGGAALTVPM
                     MTLFFLTRHLGRRRALWTLPFIWTAWEWIYHQCQFSFGGHTLANTQTNAIWLFQFMDL
                     TGVWGMIFWVVLFNVLVTQAIDDWQGQATDQAGLAVPRTHLVEKLIWVTSVMVIPPLI
                     YSAFCFWKYTKPPASDQNRLSVLMVQPNVNPWVETNPERKAAIVEKTVLLTDLAVEQT
                     SPDLIVWPEIAIPLPYRQSPEVQTFLGQAITDWNAPLLTGTLDLKSYATGMPRPKRLD
                     IEGRDFEVFNTVLALTPNWNDPQVSAPAAVVSGISAKNRLVPFVEHIPYVEHVPWLRK
                     LVARLGSNPILDQGATTTLTVQTKNQRLVKVGTQICYESLYPESSARLVREGAQFLVF
                     VTNDGWFSQTQGAEITAAASWMRAIETRRSVARCANTGKTLFFDPLGRSYGEIPWWQE
                     RTTTADILLSHDLTLYTQYGDVFPLTCLWGSALLLVALSGRTIFSKLCKEKLL"
     gene            complement(2057..>5448)
                     /locus_tag="PKZ53_02225"
     CDS             complement(2057..>5448)
                     /locus_tag="PKZ53_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39278.1"
                     /translation="NVAGNTAIGNGANGIFITNSADTNTVGGTTANDRNVISGNILDG
                     VRITTADTTGNVVIGNFIGTNLGGTAAVANQFSGVRINGCSNNRIGGTTAAERNILSG
                     NTGDGVVIIGAAATGNTVSGNFIGTDVNGTADLGNSLIGVEIIGSSGNTVGGTTAGER
                     NIISGNTQDGVSINTGPAANNLVIGNYIGTNLNGTAALANDFSGVRINGCSNNIIGGT
                     TPAHRNILSGNTGDGVVIVGAAATGNQVIGNFIGTDVNGTADVGNSLIGVEIIGSSGN
                     TVGGTTAGERNIISGNTLDGVSINTGPAANNVVTGNYIGTDLNGATAIANSFSGVRIN
                     GCSNNRIGGMTAGERNILSGNTGDGVVVLGTATGNQILGNYIGTTGTGTTDLGNLLAG
                     IEILTGSSSTEVRGNVISCNHRQGGTITALNANIYVTGSNSNTIAGNIIGLQADGSST
                     FAPNGGPRGVFLAANSNSNQIGGTTVADRNIISGHQHTGVEIASASTLNQVQGNFIGV
                     AADGTTARGNGDHGIAIAIANNNLIGGTNPGEGNVIASNGRSGVGIASGTGNRVLSNS
                     IFGNTVLGIDLGNDGVVQANDAGDGDSGGNNLQNFPVLSAAYFNGTTTQIQGTLNSTA
                     GRMFRIEFFTNPTCDGSGNGEGQTLLSSTMVTTNGSGNATINVTLAMATTLGQTVTAT
                     ATDQTSNDTSEFSACRTITLAPPTLTKSFTPDTILVGQTSVLTFTITNPNSNSGLSGI
                     SFTDPLPGGVLIANPNGLVNGCGGTVTATSGTGTITYGNGNLAASGSCTFSVTVLGAT
                     TGTKVNTTNPISAMESGPGGTATATLVVGETVEFAVLYVADTSNNRIQRFDGTTWAIV
                     GTGVVGSGNGQFRAPEAVTASPSGLLIYVADTNNNRIQWSTDGGTTWDNFATVGSAPN
                     QVRSPHGLALDRDGNLYVSDTGNGRVMRFTGGVPGAGVIIATSGAASGQVSSPRGLAI
                     NSAFELFVADETNSRILKINNANTVLTGTTGTIVATAGSALNKVQNPQGIAVDNTGNV
                     YVADTGNSRILRWANSNPASSTSLALVGTLLGQVNKAEGITVCQFASGPFAGQSSLVV
                     GDTLNNRIQGRFLASGPWALLGAPNGIGSGVGQFRAPSKIR"
BASE COUNT         1191 a         1489 c         1505 g         1263 t
ORIGIN      
        1 tgaaaaagcc agggtcaggc tgagacccag cacaatccgg ctgaggattt ttggacaggt
       61 caggttcata acaatttctc cttgcagagt ttggaaaata tggttcttcc actgagcgca
      121 accaggagca gggcggaacc ccacagacag gtcaacggga aaacgtcgcc atattgggta
      181 tagagggtca ggtcatgtga aagcaaaatg tcagcggttg tggtgcgttc ctgccaccag
      241 ggaatctcgc cgtagcttct tccaagcgga tcaaaaaaca gtgtttttcc ggtgttggcg
      301 caacgtgcca ccgagcggcg ggtttcaatt gcccgcatcc atgaggctgc cgctgtgatc
      361 tcggcgccct gggtttgcga aaaccaccca tcgttggtca caaaaaccag aaactgcgcg
      421 ccttcgcgga ccagccgggc cgaactttcg gggtagaggg attcataaca aatctgagtt
      481 ccaaccttta ccagccgctg atttttggtc tgaaccgtca acgtggtggt tgctccctgg
      541 tccaaaatcg gattacttcc gagcctggcg acgagcttgc gaagccaggg aacgtgctcg
      601 acatagggga tatgttcgac aaacggcacc agacggttct tggctgagat gcctgacacc
      661 accgccgctg gtgctgaaac ctggggatcg ttccagtttg gggtcaaggc cagcacggtg
      721 ttgaagactt caaagtcacg gccttcgatg tccagccgct tcgggcgcgg cattccggtg
      781 gcataggatt tgaggtcaag ggttccggtg aggagcgggg cattccaatc cgtgatggcc
      841 tgaccaagaa atgtctggac ttcaggtgat tggcgatagg gaagggggat cgcaatttcc
      901 ggccagacga tgaggtccgg cgacgtctgt tccaccgcca ggtcggtcaa caagacggtt
      961 ttttcgacaa ttgcagcttt tcgctctgga ttggtttcaa cccagggatt gacgttgggt
     1021 tggaccatca gaaccgaaag ccggttctgg tctgatgctg gaggtttggt gtacttccaa
     1081 aagcagaagg ctgagtagat gagcggtggg atcaccatga ccgaggtgac ccaaatcagt
     1141 ttctccacca gatgtgtgcg cggtacggcc agcccagctt gatccgttgc ctgtccttgc
     1201 cagtcgtcaa ttgcctgtgt caccagcaca ttgaacagga caacccaaaa aatcattccc
     1261 cacacaccag tcagatccat gaattggaac agccagatgg cattggtttg tgtgtttgca
     1321 agtgtgtgcc cgccaaacga aaactggcac tggtgataga tccattccca ggcggtccag
     1381 ataaaaggga gcgtccataa cgcccggcgc cggcccagat gtctggtcag gaaaaacagc
     1441 gtcatcatcg gtacggtaag tgccgcacca ccaacgaaaa agctgactgt tgccactgtc
     1501 ggtgaggtca gcagtatcca cgactgcccc acggcaaacc atgttgaaag cgcgacgaac
     1561 gacaatccaa accagcgtcg aaccgaagtt tccgacctga gtgccaccag catcggcacc
     1621 agaccaaacc aggccagaaa ttcaaatctg attccggaaa aggcgagtgc tggcagaccc
     1681 agcagcacac cggtcagaac atagggttgg agttgggcca accggacgta aaccgatggt
     1741 ttggacgaca tagacacctg cgggacagcc catttggggg tgatggttga ggctacacct
     1801 cattccaaat ggatggagac actgtggtga agaaggaacg tttttcgggc gaatgatggc
     1861 ttgaacataa gcatcctgag agcacccgaa aacaaatatt ctctcaaccg caggttttgt
     1921 cgccaaagtg tcgttttcgt cgccgaatgg tgccgattac aaaaaagaag tcttcggttt
     1981 tcccactgct gggttttcga ttttaggtca ctttcgtcct ttttgtcctt ttcgtccggt
     2041 taccgccacc aacccatcag cgaatcttgg atggtgcccg gaactgcccg acgccactgc
     2101 caatgccgtt tggtgcgcca agcaacgccc aggggccgct cgccaggaac cggccttgaa
     2161 tgcggttatt cagtgtgtcg ccaaccacca gcgatgactg accggcaaat ggcccgctgg
     2221 caaactggca gactgttatg ccttcagctt tgttgacctg acccaggagc gttccaacca
     2281 gagccagcga agttgaactg gctggattgg aattggccca gcgcagaatg cgcgaattcc
     2341 ccgtgtcggc aacatacaca ttgccggtgt tatcaactgc aattccctgt gggttctgaa
     2401 ctttgttgag cgctgacccg gcggtggcca caatcgtgcc ggtcgtgccg gtgagcaccg
     2461 tgttcgcgtt gttaatcttg agaatccggc tattggtttc atccgccaca aaaagctcaa
     2521 acgcactgtt gatggcgagc cctcgcgggc tactgacctg accgctcgcg gcgccactgg
     2581 tggcaatgat aaccccagcc ccaggaacac caccggtaaa gcgcatgacc cgcccgttgc
     2641 ccgtgtctga gacatagagg ttgccatctc gatcaagtgc cagtccgtgt ggtgaccgaa
     2701 cctgattggg cgctgaccca actgtggcaa agttatccca ggttgttccg ccgtcggttg
     2761 accactggat gcggttatta ttggtgtcgg cgacgtaaat tagtaaccct gaaggacttg
     2821 cggtgacggc ttccggcgcc cggaattgac cgtttcctga gccgaccacg ccggtgccga
     2881 cgattgccca ggttgttccg tcaaagcgct ggatacggtt attgctggta tccgccacat
     2941 agagcacggc aaattcaacc gtttcgccaa ccacgagggt tgcggtggct gtgcctcctg
     3001 ggccactttc catggcagaa atcgggttgg tggtattgac cttggtgccg gtggttgctc
     3061 cgaggaccgt tacgctgaat gtacagcttc cgctcgccgc aaggttgccg ttcccataag
     3121 taatggtgcc cgtcccagag gtcgcagtca ccgttcctcc gcatccattg accaggccat
     3181 ttggattggc gatgaggaca ccacccggca gcggatcggt aaaggaaatt ccagagagcc
     3241 cgctgttgct gttcgggttg gttatagtga aggtcaaaac tgacgtctga ccaaccagaa
     3301 tggtgtcggg cgtgaacgac ttggtcagcg ttggtggcgc gagtgtgatc gtgcggcagg
     3361 ctgaaaactc tgaggtgtca ttgctggttt gatcagtggc ggtcgctgtt acggtttgcc
     3421 caagcgtggt cgccatggcc agcgtgacat tgatggtggc gttgccgctg ccattggtcg
     3481 tcaccattgt tgaactgagc aatgtctggc cttcgccatt gcccgaacca tcacaggttg
     3541 gattggtgaa aaattcaatt cggaacatcc gacccgccgt gctgttgagc gtgccctgaa
     3601 tctgggtagt ggttccatta aaataggcgg ctgacaaaac cgggaagttt tgcaggttgt
     3661 tgccgccgct gtctccgtcc cccgcatcgt tggcctggac aacgccatca ttccccagat
     3721 caattccaag caccgtgttc ccaaaaatcg aattgctcaa tactcgattc ccggtgccgc
     3781 tcgcaatgcc aacaccactc cggccattgg aagcaatcac attgccttct ccggggttgg
     3841 taccgccaat caggttatta ttggcaatgg caatggcaat accgtggtcc ccattgccgc
     3901 gagccgtcgt gccatcagcc gccacgccaa tgaagttccc ttgaacttga ttcaacgtcg
     3961 aagcgctggc aatttcgacg ccggtatgtt gatgaccaga aatgatattt cgatctgcga
     4021 ccgtggtgcc gccaatctga ttactgtttg aatttgctgc cagaaataca ccacgcgggc
     4081 caccgtttgg cgcaaacgta ctactgccat cagcctgtaa cccaatgata ttcccggcaa
     4141 tcgtgttgct gtttgaccca gttacataga tgttggcatt aagcgccgtg atggtgcctc
     4201 cctggcggtg gttacaggaa atcacatttc ctcgaacctc agtgctgctg ctgccggtca
     4261 gaatctcgat tcctgccaga agattgccca gatctgtggt tccggttccg gtggtgccga
     4321 tgtaattgcc caaaatctgg tttccggttg cggtcccgag taccaccacg ccgtcacccg
     4381 tgttgcccga aagaatgttt cgttcgccgg cagtcatccc gccaatccga ttgtttgaac
     4441 aaccgttgat ccgcacgccg gaaaatgaat tggcaatcgc cgtggcgccg ttgaggtctg
     4501 tgccgatata gttgccggta acgacgttgt tagccgccgg gcccgtgttg atcgaaaccc
     4561 catccaacgt gttgccggaa atgatgttgc gttcgccagc cgtggtgccg ccgactgtat
     4621 ttccagaact cccgatgatt tcgacaccaa tcaatgaatt gcccacatcc gccgtgccgt
     4681 tgacatcggt gccgatgaaa ttcccgatga cttgattgcc agttgctgct gctccaacaa
     4741 tcaccacccc atcgccggta ttgcccgaca ggatgttgcg gtgtgccggc gtggtgccgc
     4801 cgataatatt gtttgaacag ccgttaatcc gaacgcctga aaagtcattg gcaagtgcgg
     4861 ctgtcccgtt gaggtttgtc ccaatgtaat tgccgatgac aaggttattg gctgcggggc
     4921 ccgtgttgat cgaaacgcca tcctgggtat ttccagaaat aatgttgcgt tcgccagccg
     4981 tggtgccgcc aactgtattt ccagaacttc caatgatttc aacgccaatg agtgaattcc
     5041 caagatcggc tgtgccgttg acgtcggtac cgatgaaatt accgctgacg gtattgccgg
     5101 ttgctgccgc tccgataatc accacgccat caccagtatt gccggaaagg atgtttcgtt
     5161 ccgctgccgt cgtccctcca attctgttat tggagcaacc gttgatccgc acaccggaaa
     5221 attgattggc aacggcggcg gtgccaccca ggtttgtccc aatgaaattc ccaatgacga
     5281 cattgccggt tgtatcagcc gtggttattc tgacgccatc caggatgttg ccggaaatga
     5341 cattgcgatc attagccgtg gtgccgccaa cggtgttggt gtcggcacta ttggtgatga
     5401 aaatgccatt cgccccattt ccaatcgcgg tattcccggc gacattga
//
LOCUS       DAOQDO010000085         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9479_length_5437_cov_3.701784, whole genome shotgun sequence.
ACCESSION   DAOQDO010000085 DAOQDO010000000
VERSION     DAOQDO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9479_length_5437_cov_3.701784"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..2469
                     /locus_tag="PKZ53_02230"
     CDS             385..2469
                     /locus_tag="PKZ53_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39279.1"
                     /translation="MNTCPICYKQFPDSIRVCPNDGVGLISPDSKTIDARSLAQQDPL
                     IGKCIADRFQILGKLGQGGMGAVYQAKHTKMNRMCAIKIMNAQIANDPEAMARFNREA
                     QMSSQLNHPHAVTIYDFGEAEGGLVYLAMEYVEGETLSKVLAKRGPLPLDMVLNIARQ
                     AGSAIEAAHRMLIIHRDLKPDNIMLTKNNGQDWIKVLDFGIAKTTDDQRHVTQTGMTI
                     GTPAYMSPEQAAGDKLDARSDLYSFVLIVYEMLTGALPFRGENSQAIMVKRLTDPPMP
                     LQVANPNVRVHPQIEAVIMQGLARVRDYRPPSVEQFVRNFEMAMAQSAGQQQFFQPGP
                     QTLPHTAPGRTAPPPPHFGTNPATPPQAQPGRPPMGQPPMGQPPMPPQGRPPSGYAQP
                     PQPPPGFSSSGPTVPANAPGAPPSLMKPPTNPPGNMPPMNTPGMPIPGQRPLAGNSLA
                     PQNTPGNLPTVPYPQGPAVQSGPNMPHPQGPPMPPTNMPPTANGRSNVGLIIGVIVIV
                     IVLGIGALLLLAALSNADGQSTQNTPGDSSSSGSGESGTSPSGSNKDKKKDKVKKETV
                     APVADLSGEWILTNTIEHTSYSAYEGLRLGYRIYLQQDGNSLKGNGEKRWENGVEISS
                     PTARTPIYLEGTISGDSVTLNITEVGAKRTTAGQITLTVHPETNTLDGRFSSNAADSS
                     GAAYIEKNSNTY"
     gene            2475..3269
                     /gene="mtgA"
                     /locus_tag="PKZ53_02235"
     CDS             2475..3269
                     /gene="mtgA"
                     /locus_tag="PKZ53_02235"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNJ39280.1"
                     /translation="MKKKHRLPDNPDQAFTAPAVRLPKGQLKKGGWFRKSLKIFGLLL
                     VLFLGYQAVTWPNVSRLKVENPATTAMIENRLAQAKADGREPKREQTWVPYNQISANL
                     KRAVLAGEDSRFFGHDGFDREAIEKAMEENWEKKDFVRGASTVSQQLVKNLYLSESKD
                     PFRKFKEAIITWQMEQSLSKERILELYLNVIEWGDGIYGAEAAARRYFKKSASGLSAS
                     EAAFLAAMIPNPRTVYNPDKNPRRVRGRQRIILRYMNGIRLPSSKS"
     gene            3374..3652
                     /locus_tag="PKZ53_02240"
     CDS             3374..3652
                     /locus_tag="PKZ53_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ39281.1"
                     /translation="MGKLDEINIRLYQDTSGHAPFRAWLNSLKDLQAKSKVNIQITRL
                     RLGNPAKAKSVGEGVLELKIDFGPGYRVYYGQDGEHMVLLLLGGDKST"
     gene            3718..3996
                     /locus_tag="PKZ53_02245"
     CDS             3718..3996
                     /locus_tag="PKZ53_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNJ39282.1"
                     /translation="MNNPQFLTGSYKEMLLEGLRNPDEAEQYINAALEEGDDTVLLLA
                     LRDVAEAYGISQMAPDQWLSKPENSHFSGLIQILNALGLQLVVKRKAA"
     gene            complement(4032..4829)
                     /locus_tag="PKZ53_02250"
     CDS             complement(4032..4829)
                     /locus_tag="PKZ53_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ39283.1"
                     /translation="MAENSHLLKIADDLEMEFVLIPPGTFLMGSSDDEVEAAFIEARR
                     YSPKANRDWIRCEQPPHQVTINQPFYLGKFQVTQAQWQIVMKTNPSRFQGATLPVERV
                     SFTDCQRFLKKLTAGNDTFRYRLPSEAEWEYACRAGTTTPFSTGMTISVNEVNYNGKY
                     PYAPAPKGKYRKKTTPVGSFSPNPWGLYDMHGNVWEWCQDRHRSRYEGAPTDGSAWEH
                     GLGEWPRVIRGGSCHSQAWYCRSASRTGNSPGHSHEALGFRVVATLR"
     gene            complement(4841..>5437)
                     /locus_tag="PKZ53_02255"
     CDS             complement(4841..>5437)
                     /locus_tag="PKZ53_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ39284.1"
                     /translation="RHRHAQFQVLPAALELGRQRLLALRADQFHRLIHRQTGTQRTGQ
                     KVERVRQLVLVSTTSAFPPKGKEALAHLAHLAETQGMRAVADAFAPRLFGSVIDLKLD
                     DRFQALAAAFTEQDPASFAAACRALALADVTPELSKIQTETLLVYGENDRQTPPFFGE
                     ALVSKLPNARMVVVPKLGHVLPIEDPKTFNRILLEFLR"
BASE COUNT         1391 a         1511 c         1333 g         1202 t
ORIGIN      
        1 gggttccggg ttccgggttc cgggttccgg gttccgggtc ttcgaattta tgtcctttat
       61 gtcctttatg tccctttcgt ccttttcttt ccctgccccc taaaacctaa aacccggaac
      121 ccgagccact tcattctggt ataccaaacc tggcttttgc ccaagccagg ttttgtattt
      181 tttactctgg ctggaacttg aaaaattgct ttcttggctc aaaaagcagt atcgtttttg
      241 gtacctgttc acttccggtt ctttccagaa acacacccag ccatttttta aaaccagttg
      301 attggaaaaa accctctttg attgcgcttc acggttgccc ggtgaccacg accaaaagcg
      361 cttcttgaaa agcgaaggcc tggcatgaat acctgtccga tttgttataa acaatttccc
      421 gactcgatac gcgtttgccc caatgatggg gtgggattga tttcacctga ttccaaaaca
      481 attgatgccc gctcgctggc ccagcaggat ccactcattg gaaaatgtat tgctgatcgc
      541 tttcagatcc tgggcaaact tggtcaaggt ggaatgggcg cggtgtatca ggcgaaacac
      601 accaaaatga accggatgtg cgccatcaag attatgaacg cccagattgc caacgatcca
      661 gaggccatgg cgcgatttaa tcgtgaagcc cagatgtcga gtcagcttaa ccatccccac
      721 gcggtcacaa tttatgattt tggtgaggct gaaggcgggc tggtctatct ggccatggaa
      781 tatgtcgaag gtgaaacact gtcgaaagtc ctggccaaac ggggaccatt gccgcttgat
      841 atggtcctca acattgcccg tcaggccggg tcagccatcg aagccgccca tcgaatgctc
      901 atcattcacc gcgacctcaa gcccgacaac atcatgctca ccaaaaacaa cgggcaggat
      961 tggatcaagg tgcttgattt cggcattgcc aaaaccactg atgatcagcg gcatgtgacc
     1021 caaaccggca tgacgattgg aacaccagcc tatatgtcgc cggagcaggc cgccggagat
     1081 aaactcgatg cccggtcgga cctctatagt tttgtcttga ttgtgtatga aatgctgacc
     1141 ggggcgctgc catttcgagg ggaaaactca caggccatta tggtcaaacg gttgaccgac
     1201 ccaccgatgc cgctccaggt tgccaaccca aatgtccggg ttcaccccca aatcgaagcc
     1261 gtgattatgc aggggctggc gcgcgttcgg gactatcgcc caccttcagt cgaacagttt
     1321 gtcagaaatt ttgaaatggc catggctcaa tcggctggac aacagcagtt ttttcagcct
     1381 ggaccgcaaa cgctgccgca tacggcgcca ggtcgcaccg cgccgccacc accgcacttt
     1441 ggaaccaatc cggccacgcc accacaggcg cagcctggac gaccaccgat gggacaacca
     1501 ccaatggggc aaccaccgat gccgcctcag ggccggccac catcaggata tgcccagcca
     1561 cctcagccac cgccaggttt ttcaagctct ggaccgaccg ttccagccaa tgcaccaggg
     1621 gcgccgccat cgttgatgaa acctccaacc aatccgccag gaaacatgcc accaatgaac
     1681 acacctggca tgccgattcc aggccaacgg ccactggcgg ggaactcgct ggcgccccag
     1741 aatacacccg gtaacctgcc gacagttccc tatccgcaag gccccgccgt gcaatccggg
     1801 cccaatatgc cacatccgca aggaccacca atgccgccca ccaatatgcc accaacggcc
     1861 aatgggcgca gcaacgtagg tctcatcatt ggggtgattg tcatcgtgat tgtacttggc
     1921 atcggcgccc tcctgctcct ggcagccctt tccaatgctg acggccagtc aacccaaaac
     1981 actccaggtg acagctcatc atctggctca ggcgaaagcg gtacctcgcc ctctgggtca
     2041 aacaaagata aaaagaaaga caaagtgaaa aaagaaactg tggctccagt ggccgatctt
     2101 tcaggtgagt ggatcctgac caacacaatt gaacatactt cctatagtgc ctatgaagga
     2161 ttgcggcttg gttatcgcat ttacctgcaa caagatggca attcgctcaa gggcaatggg
     2221 gaaaaacgct gggaaaacgg ggttgagatt tcttcaccaa ccgcccggac tccgatttac
     2281 cttgaaggaa caatcagcgg agattccgtc acgctcaaca tcaccgaagt cggcgccaaa
     2341 cgaaccacgg ctggccagat caccctaacg gttcaccctg agaccaacac gctcgacggg
     2401 agattttcca gtaacgcggc tgattcgagc ggtgccgcct atattgaaaa aaattcgaat
     2461 acttactgac gatcatgaag aagaaacacc gattgcctga caatcccgac caggcattca
     2521 ctgccccagc cgttcgcctc cccaaaggtc aactgaaaaa aggcggctgg tttcgcaaga
     2581 gtttgaaaat ttttggcctg ctcctggtct tgtttctcgg ataccaggca gtcacctggc
     2641 ctaatgtcag ccggctcaaa gtcgaaaacc cggccaccac agctatgatt gaaaaccggc
     2701 tggctcaggc caaagccgac ggtcgcgaac ccaaacgcga acaaacctgg gtgccgtaca
     2761 accagatttc cgccaatctc aaacgtgccg tcctggctgg tgaagattcc cgcttctttg
     2821 gccacgatgg gtttgaccgt gaagccattg aaaaagcgat ggaggaaaac tgggagaaaa
     2881 aagactttgt ccgtggcgcc agcaccgtgt cacagcaact ggtcaaaaac ctgtatcttt
     2941 cagaatccaa agaccctttc cgcaaattca aagaagccat cattacctgg caaatggagc
     3001 aaagcctgtc caaagaacgt attctggaac tctatctgaa tgtgatcgaa tggggtgacg
     3061 gcatttatgg ggccgaagcc gccgcccggc ggtacttcaa aaaatcagct tccgggcttt
     3121 ccgccagcga agctgcgttt ctggccgcga tgattcccaa tccacgcacg gtctataacc
     3181 ctgacaaaaa cccacggcgc gtccgtggcc ggcaacgcat cattctgcga tatatgaacg
     3241 gcatccggtt accttcgtca aagtcctgac tgctaaagat agaaatcagg aaacctatga
     3301 agaatgaatt ttttgcctca tttcaaagcg gtaacaaact gaaaatcaat aagttatggg
     3361 gacaagagaa tttatgggaa aactggatga aataaacatt cgattgtatc aagatacctc
     3421 tggtcatgct ccattcagag cctggttgaa ctcacttaaa gacttgcaag ctaaaagcaa
     3481 agttaatatt caaatcacac gcttgcgttt aggaaaccca gccaaggcaa aatcagttgg
     3541 ggagggggta ttagaattaa aaattgattt tggacctgga taccgggttt attacggcca
     3601 ggatggggaa catatggtgc ttttgttact cggcggagac aaaagtacat aatcaactga
     3661 tatcaccaag gcaaaagaat attgggcaga ttacaaatcg cgaaaaaagg aggccttatg
     3721 aacaacccgc aattccttac tggaagctat aaagaaatgc tccttgaagg gttacgaaat
     3781 cctgacgaag ctgagcagta tatcaacgca gcacttgaag aaggtgatga tacagttctc
     3841 ctgctggcac tacgtgatgt cgctgaagcc tatggaatca gccaaatggc accagatcaa
     3901 tggttgtcca aaccagagaa ctctcacttc tcaggcttga ttcaaattct gaatgcactg
     3961 ggcctgcaac ttgtggtcaa acgtaaagct gcctgaactc aaaagaatcg cctgcaaaat
     4021 atctttttgc tttatctcaa cgtcgcaacc acccgaaagc caagagcttc atgcgaatgg
     4081 ccaggtgagt tccctgtccg gctggctgag cggcaatacc aggcctgcga atggcacgag
     4141 ccaccccgga tcacccgtgg ccattcacca aggccgtgtt cccaggcgct gccatcggtc
     4201 ggcgccccct cgtaacggct ccgatgccga tcctgacacc actcccagac atttccgtgc
     4261 atgtcataga gtccccaggg attcggagaa aaacttccga cgggtgtggt ttttttgcgg
     4321 tattttccct ttggggctgg cgcatacgga tacttgccat tgtagttgac ctcattcacg
     4381 gaaattgtca taccagttga aaaaggtgtg gtcgtgccgg cccggcaggc atattcccac
     4441 tcggcttcac tgggtaaccg atatctgaat gtatcgttgc cggcagtcaa tttttttaga
     4501 aaccgctggc aatcagtgaa cgaaactcgc tccaccggca aggtggcacc ctgaaaccgt
     4561 gacgggttgg ttttcatcac tatctgccac tgagcctgag ttacctggaa ttttcccaaa
     4621 tagaaaggtt gattgatcgt cacctgatgg ggtggctgtt cacaacgtat ccagtcccgg
     4681 tttgcctttg gactgtaccg ccgtgcctcg ataaaagcgg cctcaacctc atcatctgag
     4741 gaccccatca gaaaagttcc gggtggaatc aacacaaatt ccatttcaag atcatctgct
     4801 attttgagaa ggtgtgagtt ttcagccatc acggtttcaa ctacctcaaa aactccagca
     4861 atatccggtt aaacgtcttg gggtcttcaa tcggtaggac gtgacccagt ttgggaacca
     4921 ccaccattcg ggcattcggc agtttcgaga ccagcgcttc gccaaaaaac ggtggtgtct
     4981 ggcggtcatt ctccccatac acaagcaacg tctcagtctg aatctttgac aattccggtg
     5041 tgacatcggc cagggcgagt gcccggcagg ccgccgcaaa actggctgga tcctgctcgg
     5101 taaaagctgc cgccagcgcc tgaaaccgat catccagctt taaatcaatc acgctcccaa
     5161 acagtcgggg tgcaaaggca tcggcaacgg cccgcattcc ttgcgtttca gccaggtgtg
     5221 ccaggtgcgc cagggcttct tttccttttg gtggaaaggc cgaggtcgta ctgaccagaa
     5281 ccagttgccg gacacgttcc accttctggc ccgttcgctg cgtgccggtc tgacggtgga
     5341 tcagtcggtg gaactgatcg gctcgcagag ccagaagccg ctggcgaccg agttcaagcg
     5401 ctgcggggag cacctgaaac tgggcatgac ggtgccg
//
LOCUS       DAOQDO010000086         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9480_length_5437_cov_3.023040, whole genome shotgun sequence.
ACCESSION   DAOQDO010000086 DAOQDO010000000
VERSION     DAOQDO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9480_length_5437_cov_3.023040"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKZ53_02260"
     CDS             complement(<1..279)
                     /locus_tag="PKZ53_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39285.1"
                     /translation="MTINELITNFGGKPVVDWNPGDNLTNPAETCFRISLSYDAEQEG
                     EEWPDLLSKFLENPEASQVYGIVVGNWGEMYTGTEAGFVAGALESACEY"
     gene            475..2313
                     /locus_tag="PKZ53_02265"
     CDS             475..2313
                     /locus_tag="PKZ53_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNJ39286.1"
                     /translation="MNRKISIIGIVLVLFITACGSVTKPSQAQTQSATPAVAQADEHS
                     KPGGIRWEGWSDDIFERAKRENRLVILDLEAVWCHWCHVMAKETYSDPEVIKLIGNRY
                     IAVKVDQDSRPDLSNRYEDYGWPATIVFDSNGQELAKRSGYIPPRPMATMLQAFIDDP
                     TPGPSVGAEAQLTAPESAFLTDALRKDALQVHMTGYDTKYGSWGFIHKYLEWDSVEYS
                     MMLAKDGDKAAEQRAKKSLAGELNLIDPVWGGVYQYSTGGVWTEPHFEKIMSHQAENL
                     RIYSQAYALWKDPVHLKAAQDIHKFLKNFLTSPEGAYYTSMDADLIRGEHSEAYFKLN
                     DAGRRKLGIPRVDQHVYARENGWAITGVVSLYAATGDAKYLTEALTAANWIVANRSLP
                     DGGFRHDAKDAAGPYMGDTLAMGRAFLALYSVTGDRDWLKKAEAAAQFIGKTFSHQVK
                     GGQSVGFETAKPLKMNPSKPQRDENIVMARFSNLLFHYSGKEEYRKLAEQAMRFLVNP
                     DIATRRPTGGTLLTDLELAREPVHITVVGAKDDPQAQALFRSAMSYPIGYKRVEWFDR
                     REGALPNNQVEFPELPKAAAFACAGVRCSLPVFEPKEIRPMVERLK"
     gene            complement(2427..3674)
                     /locus_tag="PKZ53_02270"
     CDS             complement(2427..3674)
                     /locus_tag="PKZ53_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39287.1"
                     /translation="MGARHLSQSALTAAYQANLGTFVNLEARLANELNCPLLLEHSGA
                     NIYRVMTRVKSLESFLTKVERRGFSGESVFSEMTDLVGARIICMFPEDLEKIHLFLTE
                     CGSFSFIGKPKAYLWDDLAWAHKEEFYITRKISGYGSIHYLARLSPSLVGLNSDLGTL
                     VFEIQVRTLMQEAWAALEHAIGYKHDIPERIRGYFDSAAELLGVLDKSFQRLKTQSDQ
                     LEGELNEENSITPERLTLFSLKGYIRRKFHDNLPGTQLAPLLEEYHHQKLNLAELVSI
                     TNQTRYLKLVDELCRDLLYRPAATYDYLHWICTFRLATNNLDEARRLIEERMRRTREY
                     QECHAGELLVSVLRQLGDRELADMLQQAHDVKLNGRQLMVLYGTGKEPAAAHTSKHTS
                     RLVEISRMIFGPINQLSVQIDGN"
     gene            complement(3881..4909)
                     /gene="hemE"
                     /locus_tag="PKZ53_02275"
     CDS             complement(3881..4909)
                     /gene="hemE"
                     /locus_tag="PKZ53_02275"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNJ39288.1"
                     /translation="MTLHNDLLLRACRQQPVERPPVWMMRQAGRYLPEYRAVRADIPF
                     MTLCKTPELAVEVSMQPLRIIGVDAVIMFSDILVPVEAMGLDVEITEKIGPRIANPIR
                     SESDIHRLRVPDPVETMPFVFEILRQLRSEIQGAVPLLGFAGAPWTLAAYMIEGGGSK
                     NYIHIKGFMHREPKAFHAMMEKVADTQALYLKAQIEAGAQAIQLFDSWAGELSPADFA
                     EYALPYIQRVFEQVGNQVPRILYLNGTSHLLELMEQSGADVLSIDWRTSLPDARRRTG
                     GRVALQGNLDPCRLLGPREKVVQSVHDILASMKDQPGFILNLGHGILPPTPVENARAF
                     VATGQGRE"
     gene            5147..>5437
                     /locus_tag="PKZ53_02280"
     CDS             5147..>5437
                     /locus_tag="PKZ53_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39289.1"
                     /translation="MKSCPKCAKTYPDEYNICPQDGQTLVHMAKKAAGDIFDHLIDDK
                     YQVLRLIGRGGMGAVYEAIHTTMQRRVAIKILNPELVSNPAAMERFRREALAS"
BASE COUNT         1388 a         1335 c         1373 g         1341 t
ORIGIN      
        1 gtattcgcaa gctgattcca gggcgcctgc tacaaaacct gcttcggtgc cggtgtacat
       61 ttcaccccaa tttccgacca caataccata cacctgactg gcctcggggt tttcaaggaa
      121 ttttgacaat aaatcaggcc attcttcacc ttcttgttca gcgtcataag aaaggctgat
      181 gcggaaacag gtttcagcag gattggtgag gttatcgcct gggttccaat caacaacggg
      241 tttacctcca aaattggtta tcagctcatt gatggtcata aaaattgatt cctgtttggg
      301 ataaaggtaa gaattcagat tgagaattgc cagaagtaaa gccttctaac tggaaaaaat
      361 caaaaccatt tccgatcacg ttcccgtaag ggggacttgt tcaaccactg aacattctgt
      421 tttttcacct gtttgattca aattcagatt tcaaaccatt ttttaggacg agatatgaat
      481 cgaaaaattt caatcatcgg catcgtgttg gttttattta ttactgcctg cggatcagtg
      541 accaagcctt cgcaagctca aacgcaatcg gcaacgccag ccgttgccca ggctgacgag
      601 cattcaaaac ccggcgggat ccgctgggaa ggctggtcag atgacatttt tgaacgcgcc
      661 aaacgtgaaa accgcctggt gattctcgat cttgaagccg tgtggtgcca ctggtgccac
      721 gtcatggcca aagagaccta ctccgacccc gaagtcatta agttgattgg caaccgctat
      781 attgcggtca aagttgacca ggattcccgg cctgatttgt ccaaccgcta tgaagattat
      841 ggctggccag ccacgattgt gtttgattcc aatggtcagg aactggccaa acgctcgggc
      901 tatattccgc cacgtccaat ggccaccatg ttgcaggcgt ttattgacga tccaacccca
      961 ggtccttcag ttggtgctga agcccaacta accgctccgg aatcagcttt tctgacggat
     1021 gcgctccgca aagatgcctt gcaggtccat atgaccggct atgacaccaa atatggaagc
     1081 tggggcttta ttcacaaata tctggaatgg gacagcgtcg aatattcgat gatgctggcc
     1141 aaagatggcg acaaagctgc tgaacagcgt gccaaaaagt cactggccgg tgaattaaac
     1201 ctgattgacc cggtttgggg tggtgtctac cagtattcaa ccggtggggt ctggaccgaa
     1261 cctcattttg aaaaaatcat gtcgcatcag gcggaaaacc tccggattta ttcgcaggcg
     1321 tatgcgctgt ggaaagatcc agttcacctc aaagccgctc aggacattca caaattcctc
     1381 aaaaactttt tgaccagccc cgaaggtgct tactacacca gtatggatgc cgacttaatc
     1441 cggggcgagc acagcgaagc ctacttcaag ctcaatgatg ccgggcgccg caagcttggg
     1501 attccgcgag ttgaccagca cgtgtatgcc cgcgaaaacg gctgggccat cacgggcgtg
     1561 gtgtcgctct atgctgccac tggtgacgca aaatacctga ccgaagccct gacggctgcc
     1621 aactggattg tggccaaccg gtcactcccg gatggtggat tccgccatga tgccaaagac
     1681 gcggcggggc cgtatatggg tgacacgctg gcgatgggac gggcgttcct ggcgctctat
     1741 agcgtgactg gcgaccgtga ctggctgaaa aaagccgaag ctgccgctca gtttattgga
     1801 aagacgtttt cacatcaggt gaaaggtggt caaagcgtcg ggtttgaaac ggcaaaaccg
     1861 ttaaagatga atccttccaa gcctcagcgt gatgaaaaca ttgtcatggc acggttttca
     1921 aatctgctct ttcattactc tggaaaagag gaataccgaa aactggctga acaggccatg
     1981 cgcttcctgg tgaaccccga tatcgcgacc cggcggccaa ctggcggaac acttctgacc
     2041 gatttggaac tggcccgcga gccggtgcat atcacggtgg ttggtgccaa agatgacccg
     2101 caagcccagg cacttttccg gagtgcaatg agctatccaa ttggatataa acgggttgag
     2161 tggtttgatc gccgtgaagg ggcactcccg aacaatcagg tggagtttcc tgaattgcca
     2221 aaggccgccg cctttgcctg tgctggggtg agatgttcgc tcccggtgtt tgaacccaaa
     2281 gaaattcggc caatggtcga gcggctcaag taatagagtc agtaccacct gcgtgagcgg
     2341 gcgggctttc aaataccacc tgctgagatg gatgggactg gcaaggcctt aacccacccg
     2401 ctcacgcagg tggtactgac ccttcctcag tttccatcga tttgcacact cagttgattg
     2461 attggcccaa agatcattcg actgatttca accaggcggc tggtatgttt tgacgtatga
     2521 gccgccgccg gttctttccc tgttccatac agcaccatca attgccggcc attgagtttt
     2581 acgtcgtggg cttgctgcaa catgtccgcc agttcacgat ctccaagttg tctgagaact
     2641 gaaaccagta attctccggc gtgacattcc tgatattcac gcgttcgccg catccgctct
     2701 tcaatcaggc gacgggcttc gtcgaggttg ttggttgcga gtcgaaaggt gcagatccag
     2761 tgcagatagt cataggtggc ggctggccgg tagagcaggt cacggcataa ctcatccacc
     2821 agtttgagat atcgggtctg attggtgatc gagaccagct ctgccaggtt taatttttga
     2881 tgatgatatt cttccaggag cggtgccagt tgtgtaccag gaaggttatc gtgaaatttt
     2941 cggcggatat agcctttgag gctaaataaa gtcagccgtt ccggggtaat tgagttttct
     3001 tcgttgagtt cgccttccaa ctggtcactt tgggtcttca agcgttggaa cgatttgtcc
     3061 agaaccccca gaagttcagc cgccgaatca aaatatccgc gaatgcgttc cggaatatcg
     3121 tgcttgtatc caatcgcgtg ttccaaagcg gcccaggctt cctgcatcaa ggtccgaacc
     3181 tgaatttcaa agaccagcgt tcccaaatct gaattcaaac ccaccagtga tggactgagg
     3241 cgtgccaggt agtgaattga cccatatccg gaaatcttgc gagtgatata gaattcttct
     3301 ttatgggccc aggctaaatc atcccacaaa taggctttgg gttttccaat aaaggaaaaa
     3361 ctgccgcatt cggtgagaaa aagatgaatt ttttcaagat cttcagggaa catacagatg
     3421 atgcgtgccc caaccagatc ggtcatttca gaaaaaactg actcgccgga aaacccacgt
     3481 cgttcaactt tggtgagaaa actctccagg gatttgactc gtgtcatgac ccggtaaata
     3541 ttggcaccac tatgttcaag caacagcggg caattcaatt cattggccag tcgagcttca
     3601 aggttgacga aggtgcctaa atttgcttga taggcggcgg ttaatgcact ctgtgacaaa
     3661 tgtctggcac ccatagagtt cccccctgaa tgaagaattg agaatgaaga atgaagaatg
     3721 aaaaatgaag aatgaagaat gaagaatgtc agtggtattt agttctgagg aaacaatact
     3781 ttcgacgaag aaccaaggat taagagccga gtgttttctc aattcttcat tcggatttat
     3841 ttcttcattc ttcattctca attcttcatt caggattttt tcattctctt ccttgaccag
     3901 ttgcgacaaa cgcccgcgcg ttttcaactg gtgttggcgg caaaatcccg tgaccaaggt
     3961 tcaaaataaa tccaggctgg tctttcattg aggccagaat gtcgtgaact gactgaacca
     4021 ctttctcgcg tgggccaagc aggcgacagg gatccagatt tccctggagg gcgactctgc
     4081 caccggttcg acggcgggca tccggcaaac ttgttcgcca gtcaatactt aaaacatccg
     4141 ctcccgattg ttccataagt tcaagcaggt gagaagtccc attgagataa agaatgcgtg
     4201 gaacttgatt tcccacctgt tcaaagactc tttgaatgta gggcaaggca tattcggcaa
     4261 agtcagccgg cgaaagctct ccggcccacg agtcaaagag ctggatcgcc tgagccccag
     4321 cttcaatttg ggcttttaag tagagggcct gggtatcagc cactttttcc atcatggcgt
     4381 gaaaagcttt gggttcccgg tgcataaacc ctttaatgtg aatatagttt tttgacccgc
     4441 cgccctcgat catataagct gccagcgtcc agggagcacc cgcaaagcca agcaggggca
     4501 ccgccccctg aatttcagaa cgtaattgac gcaaaatctc aaaaacaaag ggcatggttt
     4561 caaccgggtc cggaacgcgc aggcgatgga tgtcagattc gctccgaatc gggttggcaa
     4621 ttcgaggtcc aattttttcg gtgatttcaa catctaatcc cattgcttca accggaacaa
     4681 ggatatcaga aaacatgatg acggcatcaa ctccaataat ccgcagtggt tgcattgaaa
     4741 cttcgactgc cagttctggg gttttgcaca gtgtcataaa tggaatatcc gctcgaacgg
     4801 cacgatactc aggcagatag cgacctgcct gacgcatcat ccaaaccgga ggacgttcga
     4861 cgggttgttg ccggcaggca cgaagcaaaa gatcattgtg tagggtcatg atggatactc
     4921 atttcaataa aagagagtct ggtttgtgat tggtagcttg caggaaatga aagaatcgtt
     4981 aacttgtcaa tatcaagaat tgatttgctc tggcaagcct cgctatactg ttgcttctcc
     5041 tacgttcaat tcacagcacg atttacccag gtgagaacag cataactttc gtgtgcattc
     5101 gggcagttct gtctgcacac atggccaatg gttgagacgg cacgacatga agtcctgtcc
     5161 aaagtgtgca aagacatacc cggatgaata caacatctgt ccacaagatg gtcagacgct
     5221 ggttcacatg gccaaaaaag cggctgggga tatctttgat cacctgattg atgacaaata
     5281 ccaggttttg cgcctcattg gccggggcgg tatgggagcg gtgtatgaag cgattcatac
     5341 gactatgcag cgccgggtgg ccatcaaaat cctgaacccg gaactggttt caaacccggc
     5401 ggccatggaa cgatttcgtc gtgaggcact ggcttcg
//
LOCUS       DAOQDO010000087         5420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9538_length_5420_cov_3.130103, whole genome shotgun sequence.
ACCESSION   DAOQDO010000087 DAOQDO010000000
VERSION     DAOQDO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_5420_cov_3.130103"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..>2543)
                     /locus_tag="PKZ53_02285"
     CDS             complement(135..>2543)
                     /locus_tag="PKZ53_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39290.1"
                     /translation="KWTIFPIEKTKFLPATPMTVTQTSRGSTSPVLWIGTYGGGLIRL
                     EETGNPQLEGEEAYTATSFTPANSKLPNGFVMSLHQTVSAASHQILWIGTSYAGVVRI
                     DLTQPDTPWLILNDTSTPAMPDNTVYEILEDPAHRVYFLTNNGVAQLTERQSSSGNGL
                     EYSMLTFTTEDGLPINSCNSIAGMVDHLGRIWVGTIKGTARFDPASRLEDHTPKPLLI
                     SQTWLNGQPFGNREKPSVQPVLGLGDQSLDGVRLTYDQNHLVFEYRLVSFFKPSETRY
                     QTQLIGLDAAPSEWSPDTKKEYPALGEGRYVFRVWGRDASGNVTGPRELSFFIKPAPW
                     RTWWAYGVYTCLLVGLGYGSLRWRIKTLQRQNLQLEEKVKLRTQELSEKNDQLDHKVA
                     ELAQKNEELAQSKDELIKSYQQAEQIFSALAEVLPGTELDHKYRLDKKIGAGGFGAVY
                     QGTHLAMKRQVAVKVFRPASVNATNESLERFKLEAVSTCRVNHPNAVAVLDSGVSTGG
                     IAYLVMELLQGKSLNQLLGTHRVLSPLRCAEIVIPVCNVLAKAHSLGIVHRDIKPDNI
                     FLHQSDDGEIVKVVDFGIAKLVGDTEEIEAQNLTGTHNLIGTPDYMSPERLNHKPYDG
                     RADVYSVGVMAYQMLVGKPPFHSDSGFVGVILQHMTKNPDPLRSINPSIPEAVDRAVL
                     RALEKNPEARPTAQEFACELATAVGTTVEALSVTRLERNRLETDGKMILGNIPSTKEN
                     DAPTMIESDHSTTAIDLSNRTMVMESGPVFVGNTSGETFDSALTMIDNASPPRVEEKN
                     EDLP"
     assembly_gap    2544..2643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2644..4375)
                     /locus_tag="PKZ53_02290"
     CDS             complement(<2644..4375)
                     /locus_tag="PKZ53_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39291.1"
                     /translation="MMLISAGFNWIGLTPIQAGYLSNQATTPTESKSLPMRSSVNPPG
                     WPEGRLFLKVFGNEDGLRSGYISCLTFDQRGYLWAGTDDGAAFYDGQTWTPVNMPNRT
                     ISNWILAMLATQDGSLWFGRSDDGLSRLHNGAWTTYTPKNSGIPSQSVYCLYESTTPD
                     GKSCLWIGTDQGLARFLDNQWTVFTTQNSGLAGNKIQCVYESTLPGKPTVLWVGTETG
                     LSRIERFHDIQPTPSPSGTGSGWTNFTTQNSKLPGDRVIKVLDTITPDGQWNFWIVTY
                     HGLVLIEPVSAPSPAHMMPSREPTDTRWLVFTTQNMLPPGMDVMSVTETRSATGKRVV
                     WIGADLGLVRLTEKPGATATGIDRFTRDIYTVHNSNLPIDGINQILQTPSPTGNPMLW
                     IATRGGGVACYREDWWTTFDTDYPGLPNNLIDSLLETTSAAGNPVLWVGTDENGLVRY
                     EAGKVINFDPKNSPLPDISITCLLETVSPAGRPAVWIGTKGNQVILLEEPTRVSAEGP
                     NPGDFSWTIFDLNTFGLSPGIINDLMEVTLPSGQRVLWVASDGGLARYEQGKWTIFPI
                     EKTKFLPATPM"
     gene            4847..>5420
                     /locus_tag="PKZ53_02295"
     CDS             4847..>5420
                     /locus_tag="PKZ53_02295"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ39292.1"
                     /translation="MKLLGELILFITIVTFPLAVFGQADVATATIRGTVTDESGSPLA
                     NASITAKNNERGFSRSVLTSEAGEYQLPFLQPGTYDLVIEANGFGGKAIKNIELTVGQ
                     ITVQNLQLQIAAIAGEVIEITADVPVIDVARTQQANTIQGRQIQSLPNLSREFTSYVY
                     TLPGVSDSNAPRAQFVGFDVSFTSSGFSIGG"
BASE COUNT         1188 a         1365 c         1401 g         1366 t
ORIGIN      
        1 caaatcagaa gaatcaatgc gtcagtacca cccgcgtcag tgggtgggcc ggctggcttc
       61 caactccatc cgccgatgcg ggtggtactg acaaatcctt tatttccagc accaacttag
      121 atgaatgccg ctccctaagg caaatcttcg tttttttctt ccacccttgg cggcgatgca
      181 ttgtcaatca ttgtcagcgc actatcgaag gtttcacctg acgtgttccc gacaaaaact
      241 gggcccgatt ccatcaccat ggttcggttt gaaagatcaa tcgccgtggt tgagtggtct
      301 gattcgatca tggtgggggc atcgttttct ttggtggatg gaatgtttcc aagaatcatt
      361 tttccatcag tctccaggcg attgcgttcc aggcgagtga ccgaaagggc ttcaacggtt
      421 gtgcccaccg ccgtggccag ttcacaggca aattcctgag cggttggacg tgcttccggg
      481 ttcttttcaa gtgcccgcag taccgcccgg tctacggcct ctgggatgga tggattgata
      541 ctgcgcaatg gatcagggtt tttggtcata tgttgcaaaa taaccccgac aaaccctgaa
      601 tccgaatgaa atggtggttt tccaaccagc atctggtagg ccatgactcc gacgctatac
      661 acatccgccc gtccatcata gggtttgtgg ttgaggcgtt ccggagacat atagtccggc
      721 gtcccaatca aattatgtgt gccagtcagg ttttgagctt cgatttcttc ggtgtcaccg
      781 accagcttgg caattccgaa atcaaccact ttgacgattt caccgtcatc actctgatgg
      841 agaaagatgt tgtcgggctt gatgtcacga tggacaattc ccagtgagtg agctttggcc
      901 aacacattgc acaccggaat cacgatttca gcacagcgaa gtggtgacag cacccgatgc
      961 gtgcccagca actggttaag tgatttgccc tgcaggagtt ccatgaccag ataggcaatg
     1021 ccaccggtgg aaacaccgga atcaagtacc gccaccgcat tggggtgatt gacgcgacag
     1081 gtcgagacgg cctccagttt gaaccgttcc agactttcat tggtggcatt gacgctggcc
     1141 ggacgaaaga ctttcactgc cacctggcgc ttcattgcga gatgggtgcc ctggtacacc
     1201 gcgccaaacc caccggcccc gatctttttg tccagccgat atttatgatc cagttccgtt
     1261 ccagggagca cttcagccag ggcactgaaa atttgctcgg cttgctggta tgacttgatc
     1321 aattcatctt tggattgggc cagctcttcg tttttttgtg ccagttcagc gactttatgg
     1381 tcaagctggt catttttctc tgaaagttcc tgggttcgaa gcttcacttt ttcttcaagt
     1441 tgcaggtttt gccgctgtaa ggttttgatt cgccagcgca ggctgccata cccaagcccg
     1501 accaggagac aggtatagac gccatacgcc caccacgtcc gccagggagc cggtttgata
     1561 aaaaaggaaa gctcacgcgg tccagtcaca ttgccgctgg catcgcggcc ccacacgcga
     1621 aacacatagc ggccttcacc aagcgccgga tattcttttt tggtatctgg cgaccattcc
     1681 gaaggcgcgg catcaaggcc aatcagttgc gtctggtacc tggtttccga tggtttgaaa
     1741 aaactcacca gccggtattc aaaaacaaga tggttctggt cataggtcag gcggacgcca
     1801 tccagcgact ggtcgccaag tccgagcact ggctgaaccg atggtttttc ccgattgcca
     1861 aatggctgtc cattgagcca ggtctggctg atcaacagtg gtttgggggt gtggtcttcc
     1921 agccggctgg ccgggtcaaa ccgggcggtg cccttgatgg ttccaaccca gatgcggccc
     1981 agatgatcca ccatcccggc gatgctgttg caggaattga tcggcaggcc gtcttcagtg
     2041 gtaaaggtca acatcgaata ttcgagaccg ttcccagaac tggattggcg ttcggtcagt
     2101 tgcgccaccc cattgttggt caaaaagtag actctgtggg ccgggtcttc gaggatttca
     2161 tacacggtgt tgtccggcat ggccggggtt gaggtgtcgt tgaggatcag ccagggagta
     2221 tcaggctggg tgagatctat cctgacgacc ccggcatacg aggttccaat ccacaaaatc
     2281 tggtggctcg cagccgaaac cgtctggtga aggctcatca caaacccatt cgggagcttt
     2341 gaatttgccg gggtaaagct cgtcgcggtg taggcttctt ccccttcaag ctgtggattg
     2401 ccggtttctt ccagtcgaat cagcccacct ccataggttc caatccagag caccggcgat
     2461 gttgatccac ggctggtttg agtgaccgtc atgggagtgg ctggcagaaa tttggtcttt
     2521 tcaatcggaa atatcgtcca tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnntcatggg agtggctggc agaaatttgg tcttttcaat cggaaatatc gtccattttc
     2701 cttgttcgta gcgggcaagt cctccgtcag aagccaccca taaaacacgc tggcctgaag
     2761 gcagggtaac ttccatcaaa tcgttgatta tgccaggaga gagaccaaat gtgttcaaat
     2821 caaaaatcgt ccaggaaaaa tcacctgggt tggggccttc ggctgagacg cgggttggtt
     2881 cttcgagcaa tattacctga ttgcctttcg tgccgatcca cacggctggt cgtccggctg
     2941 gtgaaactgt ttccagcagg caagtgattg aaatatcggg gagcggcgag ttttttgggt
     3001 caaagttgat gacctttccg gcttcatacc gcacaaggcc attttcgtca gttccaaccc
     3061 ataagactgg gttcccggct gcggatgtgg tttcaagcag gctgtcaatc aagttattcg
     3121 gcagccctgg ataatcggtg tcaaacgtgg tccaccagtc ttcgcgataa caggcgacgc
     3181 caccacctcg ggtggcaatc cagagcatgg ggtttccagt tggtgagggt gtttgcagaa
     3241 tctgattaat gccgtcaatc ggcaagttcg aattgtgaac tgtgtagata tccctggtga
     3301 atcggtcaat cccggttgcg gtggcgcccg gcttttcggt caatcgaacc aatccgaggt
     3361 cagcaccaat ccacaccact cgttttccgg tagctgatct ggtctcggtc accgacatga
     3421 catccatacc gggaggcagc atattttgag ttgtgaatac cagccagcgg gtgtcggtgg
     3481 gctcacggct gggcatcata tgtgccgggg acggagctga aaccggctcg atcagaacca
     3541 ggccatgata ggtgacaatc cagaaattcc actgtccatc gggggtgatt gtgtcaagca
     3601 ctttgatcac cctatctccc ggaagtttcg aattttgagt cgtgaaattg gtccagcctg
     3661 acccggtacc tgaaggagat ggtgttggct gtatgtcgtg aaaccgctca atccgcgaaa
     3721 gcccggtttc tgttccaacc cataacacgg tcggtttgcc aggcagtgtg ctttcataca
     3781 cacactggat cttattccct gccagccctg aattttgagt tgtgaaaaca gtccactggt
     3841 tatccaggaa acgtgccagc ccttgatctg tccctatcca gaggcaggat tttccgtctg
     3901 gagtcgtact ttcatacagg caatagacac tttgacttgg aatccctgaa ttttttgggg
     3961 tataggttgt ccaggcgcca ttgtgaagcc ggctcaatcc gtcatctgac ctgccaaacc
     4021 agagactgcc gtcctgtgtg gccagcatgg ccaatatcca atttgaaatt gtccggttgg
     4081 gcatattaac tggcgtccac gtttggccgt cataaaaggc cgccccgtcg tcagttcccg
     4141 cccagagata gccgcgctga tcaaaggtca ggcaactgat atagcctgac cggagaccgt
     4201 cttcattgcc aaagactttc aaaaacaacc gaccttccgg ccagcccggc ggattgaccg
     4261 aggaccgcat cggcaacgat tttgactcag ttggcgtggt cgcctggttg gaaaggtagc
     4321 cagcctgaat tggtgtcagc ccaatccagt tgaatccggc acttattagc atcatccacg
     4381 cgacacacgt gaggcaccat accctgaagc gtttcatatc gccctttaag tgtcttaatt
     4441 gtaagctgtt gataaattga gtgttatgag atacaggaga ctggattcaa cgaaagctgt
     4501 gtgaagcggc atgaagctta cccgaaagtg gagcaccatt aaaccctttc attgtgtcga
     4561 atgaattctc acgtgcggtt ggcagtgtat tctgtgatca gagtgtcgtt atattgacac
     4621 tttattcgtt atcttgtaca agggtgagta tcgagacctg gaatccccct tgttcaatcc
     4681 aggtaatcct catcgtctca ggttttttcc gttttcttca aaacgtgctc aggcaggaaa
     4741 acttgagctg tgtactcaaa tttcttcaga ggatcaggca gccggatttc ccatcccatc
     4801 aggttgctgt gctcgtcaaa catctccgcc aaaggacctg atatttatga aactcctcgg
     4861 tgaactgatc ctgtttatta caattgttac attcccgctg gctgtgtttg gccaggcgga
     4921 tgtggccacg gcaacgattc ggggaactgt gactgatgaa agcggatcgc cgctcgcaaa
     4981 tgcttcgatt accgccaaaa ataacgaacg cggtttttcc cgttcggtgc tgaccagtga
     5041 ggcgggcgag tatcagctcc cgtttctgca acctggcacc tatgatttag tgatcgaagc
     5101 caatgggttt ggcggcaaag cgattaaaaa cattgaatta accgttgggc aaatcacggt
     5161 tcaaaacttg cagttgcaga ttgccgccat tgccggtgaa gtaattgaaa ttacggctga
     5221 tgttccggtg attgatgtgg cgcgtaccca acaggcaaat accattcaag ggcgtcaaat
     5281 ccagagcctg ccaaatttga gtcgcgaatt tacgtcttat gtctacactt tgcctggggt
     5341 ctcggattcg aatgcgccac gtgcccagtt tgtcgggttt gacgtgagtt ttacctcatc
     5401 gggcttttcc atcggcggtg
//
LOCUS       DAOQDO010000088         5416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9550_length_5416_cov_3.699869, whole genome shotgun sequence.
ACCESSION   DAOQDO010000088 DAOQDO010000000
VERSION     DAOQDO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9550_length_5416_cov_3.699869"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..98
                     /gene="kdpF"
                     /locus_tag="PKZ53_02300"
     CDS             33..98
                     /gene="kdpF"
                     /locus_tag="PKZ53_02300"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNJ39293.1"
                     /translation="MVVTILLLAYLVVALLRPERF"
     gene            102..1874
                     /gene="kdpA"
                     /locus_tag="PKZ53_02305"
     CDS             102..1874
                     /gene="kdpA"
                     /locus_tag="PKZ53_02305"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNJ39294.1"
                     /translation="MTFNGWLQIGLFFLVIVALTKPVGLYLARVFSGEQTMLDPVCKP
                     LERIMYRLTGVDEAEEMPWTTYALAMLVFSVVSMVLLYALQRLQGLLPLNPQQFAGVA
                     PDLAFNTAASFTTNTNWQAYGGETTMSYLTQMAGLAYQNFASAAVGIVLAIAFIRGIA
                     RRESNSLGNFWVDLVRCTLWVLLPLSLVMAVFLVSQGVVQNLNPYTTVALVEPQTVQV
                     TNPDGSVTIKQIAEQVIAQGPVASQEAIKMIGTNGGGFFNANSAHPFENPTPLTNFLQ
                     MVAIFVLSAGLTYTLGHMTGSPRHGWAVFGAMAVLFLVGTTVAYWAEARGNPLLAGTN
                     QTVSAQQSGGNMEGKEVRFGIANSALFATVTTDASCGAVNAMHDSFTPLGGLVPLANI
                     MLGEVVFGGVGAGMYGILIFVVLTVFIAGLMVGRTPEYLGKKIEAFDVKMAMLAVLIF
                     PLVVLAFSAISSVSSGPLASIANPGPHGLSEIVYAYTSAAGNNGSAFAGISVNTFWYN
                     TTLGFSMLIGRFFVIIPMLALAGNLGRKKSIPPSLGTFPVTTPLFTTLLVSVIVIVGA
                     LTFFPMLSLGPIVEDLLMQAGKTF"
     gene            2487..2765
                     /locus_tag="PKZ53_02310"
     CDS             2487..2765
                     /locus_tag="PKZ53_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNJ39295.1"
                     /translation="MTKAELVDEVARAAELTKKDSEVVVEEVFRSIIDCLNKGEKIEL
                     RGFGSFRIRNRGPRRGRNPKTGESVDIPAKAVPYFKPGKELKELINGE"
     gene            2980..3486
                     /locus_tag="PKZ53_02315"
     CDS             2980..3486
                     /locus_tag="PKZ53_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNJ39296.1"
                     /translation="MDHLWTPWRYQYIKDEKRLTECPFCTVVQEEQDEKNFILYRAHH
                     NFVILNIFPYTGGHLMVIPYQHIAELVALDKEVSDELMDLTKLCNQILQQEYHPHGCN
                     LGMNLGRAAGAGVAAHLHMHILPRWMGDVNFITTVAETRTVPEDLITTYRRLHPYFQQ
                     LSSIQHCV"
     gene            3664..>5416
                     /locus_tag="PKZ53_02320"
     CDS             3664..>5416
                     /locus_tag="PKZ53_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ39297.1"
                     /translation="MSKTERIRDILDPVAHQLQNPQELDRVLYQIAREATEYFDAVCC
                     RVWLIRRGDLCATCHWADQCSDKRRCLHLKASCGAPIDSEFRRAPLDVFSHETIARGG
                     IADWSKRAPEFDFLIDRAWAEQQQLVSFATQPLRVENRVVGLMAIFSRKLIYPADYRE
                     MGAFGSYASTAIRVAELTVRAQRAETTIKEKDRELQQTTRLLNSVLNADTEQSVIVED
                     LEGNVLAFNEGASQAFGYVPNEVIGKINSEKLYAPEDRATGRMMEIFKTTIEQGTYEG
                     ILRRLRKNGEVFVEKATLTLQRDEEGDPAGFLIVARELSKTAVDASAAPTLDSALQPV
                     VVIDPLTESFQALVSSQRIDQLPDVVLGQVSRLLPVKQAAFFLVENKKFMLRAMLDGD
                     LSAIQAGWKLDPELARVWYQHLSDDRPVELSPDQVPATAPFLVGTPPLAGWRAFPLFH
                     DGLMAVVLVAPLADGGLTEAELETGRQLMFLATRVLDHALLMEQLNDQLRHLHARYSS
                     LEQEHRLLCDQSTDLQLSRAQLLEQSGNIEANLVRMTGQHRALSQEVSELRGYSAELE
                     QALESFRERQKALQNEIN"
BASE COUNT         1262 a         1371 c         1450 g         1333 t
ORIGIN      
        1 ccgtcctcat cagcctgctg tccgtcatct tcctggtcgt gacaattttg ctgctggcct
       61 atctggtcgt ggcgctgtta cgtcctgaaa ggttttgaca tatgacattc aatggatggc
      121 tccaaattgg gttgtttttc ctggtaattg tggctttgac caaaccggtt ggtttgtacc
      181 tggcccgtgt tttctcaggt gaacaaacca tgcttgatcc ggtttgcaaa ccactggagc
      241 ggatcatgta tcggctaacg ggtgtggatg aagctgagga aatgccctgg acgacctatg
      301 cgctcgccat gctggtgttt agcgtcgtgt caatggtgct gctctatgcc ctgcaacggt
      361 tacagggatt gctacccctt aaccctcaac agtttgcggg tgtcgccccg gatctggctt
      421 ttaatacggc agcttcgttt acaaccaaca ccaactggca ggcgtatggt ggcgagacaa
      481 cgatgagtta tctcacccaa atggcagggt tggcctatca aaattttgct tcggcagcgg
      541 ttggaattgt tctggcgatt gcttttatcc gggggattgc ccgacgcgaa agcaattcgc
      601 tcggcaactt ctgggttgat ctggtgcgat gcacgctgtg ggttttgctc ccactgagcc
      661 tggtgatggc ggtgtttctg gtttcacagg gtgtggttca aaacctcaac ccatacacca
      721 cggtggcgct ggttgaaccc caaaccgttc aggtcaccaa tcctgatgga agtgtcacca
      781 tcaaacaaat cgctgaacag gtcattgccc agggaccagt ggcatcacag gaagccatca
      841 aaatgattgg caccaatggt ggaggatttt ttaacgccaa cagtgctcat ccatttgaaa
      901 atccaacccc gttgaccaac ttccttcaaa tggtggctat ttttgtgctc tcagccgggt
      961 tgacctatac gctgggacat atgacgggct ctccacgtca cggatgggcc gtgtttggcg
     1021 cgatggcggt gctcttcctg gttggaacga ccgtggcgta ttgggctgaa gcccgtggaa
     1081 acccacttct ggccggaacc aatcaaaccg tgagcgcaca gcaatcgggc ggcaatatgg
     1141 aaggcaagga agtccgcttt ggaattgcca attcggcgct atttgccacc gtgaccaccg
     1201 atgccagttg tggagctgtg aatgccatgc acgacagttt cacgccactc ggaggactgg
     1261 tcccactggc caacattatg ctgggcgaag tggtctttgg tggcgttggc gccggaatgt
     1321 acggcattct gatttttgtc gtgctgacag tatttatcgc cggactgatg gtcgggcgaa
     1381 caccagaata cctgggcaaa aagatcgaag cttttgatgt gaagatggcc atgctggcgg
     1441 tgttgatttt cccgctggtt gtcctggcgt tttcagccat ttcaagcgtc agttctggac
     1501 cgctggcctc aattgctaat ccaggaccgc atggactgag tgagatcgtg tatgcctaca
     1561 cctcggcagc cggtaacaat ggttcggcct ttgccggaat cagcgtcaat accttctggt
     1621 acaacaccac gcttgggttc tcgatgttga ttggacgatt tttcgtcatt attccgatgc
     1681 tggctctcgc tggaaacctg ggtcggaaaa agtcaattcc accatcactt ggcacatttc
     1741 cagttaccac accgctgttc accacgttgc tggtcagtgt gattgtgatc gttggagctt
     1801 taactttctt tccaatgttg agcctgggtc cgattgtcga agatttgttg atgcaggcgg
     1861 ggaagacctt ctagtgcctg gtgcttagta cttggtgctt ggtgcttagt acttggtgct
     1921 tggtgcttgg tgcttagtac tttggaacca atgccttcca agaacgaaga acgaagaacc
     1981 aagaaccaag aacgaagaac gaagaaccaa gaacgaagaa ccaagaacga agaacgaaga
     2041 accaagaacg aagaacgaag taccaggcac gttcttcgtt ccaaagcggc gcacacctta
     2101 ctgaatcatg tgctggaatg caatttctga cacgaaaaga gatgggagat ttttcacttt
     2161 ttgagggcct tttcgaaagc catcagggca ggggagagcc cagaattggc aagatctctt
     2221 gattttctca taacttggta cgaataatag acttgcagaa acattgcaca atctgttagc
     2281 ttcacgttca gttacgttct caaaaacaat catatacagc actggttttc tttacggaga
     2341 ggcccggttg gcgggccacg gctgggtgga tacttttttg tgttcggtca gtccggaaaa
     2401 aaacgagttt gttgcaaaca gtttgtgaaa cggggcgctc aagccctgat cttaattttg
     2461 aaatcttgtt caagtggagg atggtcatga ccaaagcaga gctggtggat gaagttgcac
     2521 gtgcggcaga actcaccaaa aaagattcag aagttgttgt cgaagaagtg tttcggagca
     2581 tcattgattg cctgaacaaa ggtgaaaaaa tcgagttgcg cgggtttggt tcattccgga
     2641 tccggaatcg tggtccacgc cgcggacgca accctaaaac tggagagtcg gttgatattc
     2701 cagccaaggc cgtgccgtac ttcaagccgg gtaaggaact caaggaatta atcaacggcg
     2761 agtaactgca acaacccagg gctgagggcc tggggctgaa gattcgcaag ctcagggcaa
     2821 ttgaagggat gagggatgaa ggatgaggga tgaaagaaaa ccagttcttc aacccgaagt
     2881 cttcaacccg aagtcttcag cccgagaatc ttcagcccaa agtcttcagc ccgagaatct
     2941 tcagcccgaa gtcttctccc cattttctgg ccattcgata tggatcattt gtggacaccc
     3001 tggcggtatc aatacatcaa ggatgaaaaa cggctgaccg agtgtccgtt ttgcacagtt
     3061 gttcaggaag agcaggatga gaaaaacttt attctgtatc gtgcccacca caacttcgtg
     3121 attctcaaca tttttccata taccggtggt catctgatgg tgattcccta tcaacacatt
     3181 gccgaactgg ttgcgctgga caaagaagtc agtgacgaac tgatggatct gaccaaactc
     3241 tgcaaccaaa ttttgcagca ggaatatcat ccccatgggt gcaacctggg gatgaatctt
     3301 ggccgcgcgg cgggcgccgg agtcgccgcc cacctgcata tgcacatttt gccccggtgg
     3361 atgggggatg tgaatttcat caccaccgtt gcggaaaccc gcactgttcc cgaagatttg
     3421 ataaccacct atcggcgact gcacccatat tttcagcaat tgtcgtcgat tcaacattgt
     3481 gtctaacggc tcctgcgtca gttaaaaaga atgcggactg gcgaacgaaa cgcctcccac
     3541 cgtgtagctt accccttcag accctgaccc aactgcttca gccctgagcc cgagttctgc
     3601 agcccgaact cttcagcccc aattttgaac taccagtttg ttcttcaatg gttcaacatg
     3661 cctatgtcga agaccgaacg cattcgcgat attctcgatc ccgtcgctca tcaacttcaa
     3721 aatccgcagg agttggaccg ggtgctgtac cagattgccc gtgaagccac cgaatatttt
     3781 gatgcggtgt gctgtcgggt ctggctcatt cggcgcggcg atttgtgcgc cacctgccac
     3841 tgggccgatc aatgttccga caaacgtcgt tgcctgcact tgaaagccag ttgcggcgca
     3901 ccgattgaca gtgagtttcg ccgggcacca cttgatgtgt ttagccatga gaccattgcg
     3961 cgcgggggaa ttgctgactg gtcgaagcgg gcacccgaat ttgatttttt gattgaccgt
     4021 gcctgggctg agcagcaaca actggtatcg tttgccactc aaccgctacg tgttgaaaac
     4081 cgcgttgttg gattgatggc aatttttagc cgtaaactca tctatccggc tgattaccgt
     4141 gaaatggggg cgtttggctc gtatgcctca accgcgattc gcgtggccga actcaccgtc
     4201 cgtgcccagc gggctgaaac cacaattaaa gaaaaagatc gggaactcca gcaaaccacc
     4261 cggttgctca attcggtgct caatgccgat accgagcaat cggtgattgt tgaagacctg
     4321 gaaggcaacg tgctggcctt taatgaaggc gccagtcagg cgtttgggta tgttcccaat
     4381 gaagtaatcg gaaaaatcaa cagtgaaaag ctgtatgcgc cagaagatcg ggccaccggg
     4441 cgcatgatgg aaatttttaa gaccaccatc gaacagggca cctatgaagg cattcttcga
     4501 cggctgcgca aaaatggcga agtctttgtc gaaaaagcga ctttgaccct ccagcgggat
     4561 gaagaaggcg acccggctgg ctttttgatt gtcgctcgcg aactttcaaa aaccgccgtt
     4621 gatgcttctg ccgccccaac actggactcg gctttgcaac ccgtcgtggt gattgacccg
     4681 ctgactgaga gttttcaggc actggtttcc agtcagcgta ttgaccagtt gccagatgtt
     4741 gtcctgggcc aggtctcacg gttgctgcca gtcaaacagg cagcgttttt tctggttgag
     4801 aacaaaaaat ttatgctgcg tgccatgctg gacggcgatc tgagcgccat ccaggctggc
     4861 tggaagctgg atcctgaact ggcccgggtc tggtatcagc acctgtccga tgaccggcca
     4921 gttgaactat cgcccgatca ggtgccggcc actgccccat ttctggtcgg gacacccccg
     4981 ctggctggct ggcgagcctt cccgctgttt catgatggcc tgatggctgt ggtgctggtg
     5041 gccccgctcg ctgatggggg attgaccgaa gccgaacttg aaactggccg gcagttgatg
     5101 tttttggcga ccagagtgct cgatcatgcg ctgttgatgg aacaactcaa cgatcaattg
     5161 cgccatctgc acgcccggta ttcctcgctt gagcaggaac atcgcctcct gtgcgaccag
     5221 agcaccgacc ttcagctttc acgggcccaa ctcctggagc aaagtggcaa tatcgaagcc
     5281 aatctagtcc gcatgactgg acagcaccgg gcgctttccc aggaagtttc tgaactaaga
     5341 ggctattcgg cagagcttga acaggcgctt gaatcattcc gggaacgcca gaaagcactc
     5401 caaaatgaaa tcaacg
//
LOCUS       DAOQDO010000089         5410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9580_length_5410_cov_2.870401, whole genome shotgun sequence.
ACCESSION   DAOQDO010000089 DAOQDO010000000
VERSION     DAOQDO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9580_length_5410_cov_2.870401"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5277)
                     /locus_tag="PKZ53_02325"
     CDS             complement(<1..5277)
                     /locus_tag="PKZ53_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNJ39298.1"
                     /translation="MEPGTRNPEPGTLGFTGFLRHQLAEVLETHPEHIPVDRSLVELG
                     IDSLIALELIRTLQEQFGLKLTPGDIFLRPTIEKLAAFLTSEFEKLPAPSLTSSAQCL
                     QPNIFSPELASLQPDVFSPQSGVLSYNQRSLWFMQLLEPKSTAYHIVNAVRIGSPVNV
                     SAFEQAFQYLVDRHEALRTTFPMVKGKPSQQVHSTMNVSFHAVAAGQWSDEQLQQALL
                     DEAHRPFDLSRESLLRVTLFSRQTDDHILLLTLHHIIADVWSLAVLLDEFAQIYQAAT
                     QGKTIQLPAPASTPIQHALAQMAMLDSPEGDRLWNFWKTTLALPLPVLNLPTDHPRTS
                     VQRMIGATQVFQIDQETTRQLKALSLANHTTLYVTLLSAYFVFLHQLTRQEDVIVGSP
                     MAGRNQPELTGLVGYLVNPVPLRVNCAGDPMFLEFLERVRQTVVQGLDHQDFPLALLV
                     ERLQLERDLSRSPIFQTMFTLQKVHLPHLADLLSVGLGGKAGICLGGLEMTAWPVPRR
                     IAQFDLDLMMAEVDDALVAILEYNTDLFEGASIPHLFDQFHTLLQSLASHPDELHITT
                     MTPVPKPFSTPLHSHANLVEVLQFRARHQPDRLAYTFLVDGESEEATLTYLEMDRQAR
                     AIAAKLQSVAPRDARVMLLFNPGLEYVTAFFGCLYAGMTAVPAYPPRPNRGLSRIQSI
                     VADAEASVALTSAALLAKTQTMMVHTPDLQKLAWVATDSIEAGWENEWKPVSLSPEDL
                     AFLQYTSGSTGIPRGVMVSHANIMQNEEAMRRCMECAPERPGVSWLPPYHDMGLIGML
                     QAVYTGFHGVLMAPTAFLQKPLRWLKAISKYRGFVSGAPDFAYDLCARKITEEEKAGL
                     DLSCWEVAYNGAETIRSETVNRFAESFRSCGFHTEAMHPCYGLAEATLIVSGGRKPTG
                     PAVKTILSEPFKYNRIEAAEHETDQTRSLVGCGVNLFEQQIVIANPDTLTRCQDLQVG
                     EVWVAGKTVARGYWNRPEETRSTFQAFLADTGEGPFLRTGDLGFLDAGELYVTGRIKD
                     LIIIHGQNYYPQDIELTVERSHRSLRSISGAAVSVEIGGVERLVIVQELEFRQKPDLN
                     EVITAIRQAVAKEHELQCHAVAIIKPGSIAKTSSGKIRRHACKKAFLANELEIVDGGL
                     WQAAFEPVSIPAPAAELPVTPTLKAHPGTVHTIARWLVEQVAQLAKIPATEVDVRQPF
                     AFYGLSSIDAVNLAGDLEKVLGIPISPTLVWDYPTIDAVSNHLATLENLTFHPPQKSE
                     TAEAIAIVGIGCRFGGHASSPEAFWDLLKNGVDAVTEIPPSRWDVNAFYDPDPAAPGK
                     SNCRFGSFVDHFDEFDPLFFNISPREAACIDPQHRVLIETTWEALESAGLPPNRLAGS
                     ETGVFVGISSNDYSLVQARYANREQTVEAYTGLGNAHSIAANRLSYLFNLRGPSMAVD
                     TACSSSLVAVHLACQSILNGECNLAIAGGVNLILSPEPTISFSKARMLSPDGRCKTFD
                     AAANGYVRGEGCGVIVLKRLSDAQRNGDHILAIIRGSAINQDGLTNGLTAPNGPSQQA
                     VIRKAIKAARVSPTDISYVEAHGTGTPLGDPIEVQALAAVISENRPESQPCVIGSVKT
                     NLGHLEAAAGIAGLIKTVLALHHRQIPPHLHFQQPNPNLSLEPNKLTVPRTLTTWNPI
                     NHKRLAGVSSFGFGGTNAHIILEEAPALLPQSGRDQTTHVLTLSAQSESALRRLAGEY
                     SEFLTRQPGHLVGNVCFSANTGRALFSHRLTIL"
BASE COUNT         1228 a         1412 c         1582 g         1188 t
ORIGIN      
        1 caggattgtc agccgatggg aaaataacgc ccggcctgtg ttggctgaaa aacacacgtt
       61 gccgaccaga tggccaggtt gtcgtgtcaa aaactcgctg tactcgcccg ccagtcgccg
      121 caaggctgat tcactctgag ccgaaagcgt cagaacgtgg gttgtctggt cacggcctga
      181 ttgcggaagc agggccgggg cttcttcgag gatgatgtgg gcattggtgc cgccaaatcc
      241 aaatgaacta actccggcca ggcgcttgtg attgataggg ttccaggtgg tgagtgtgcg
      301 tggaacggtc agtttgttcg gttcgagtga caggtttgga ttcggttgct gaaaatgcag
      361 gtggggcgga atttgccgat ggtgcagggc cagaacggtt ttgatcagtc cggcaatccc
      421 ggcagcggct tcgaggtggc ccagattggt tttgaccgaa ccaatcacac agggctgtga
      481 ttcggggcga ttttcactga tgacagcggc cagcgcctgg acttcaatcg ggtcgccaag
      541 cggggtgcct gtgccgtggg cttcaacata gctgatatcg gtgggtgaaa ctctggcggc
      601 tttgatggcc ttgcgaatga ccgcctgttg tgacgggcca ttgggcgccg tcaacccatt
      661 ggtcagcccg tcttgattga tggccgaacc acgaatgatc gccaggatgt gatccccgtt
      721 gcgttgggcg tcggaaagcc gtttgaggac aatcaccccg cacccttcac cccggacata
      781 gccattggct gctgcgtcaa aggttttaca gcggccatcg ggtgagagca tccgggcttt
      841 tgaaaatgaa atcgtcggtt ccggcgacaa aatcagattg accccgccgg caatcgccag
      901 attacattcg ccgttgagaa tgctctgaca ggcgaggtga accgccacca gcgatgacga
      961 gcaggccgtg tccaccgcca tgctcggccc gcgcaggtta aacaggtatg acaggcggtt
     1021 ggcggcgatg ctgtgcgcgt tgccaagtcc ggtgtaggct tcgacggttt gttcgcggtt
     1081 tgcatagcgc gcttgcacca gcgaataatc attggaactg atgccgacaa acacgccggt
     1141 ttcactgccg gcgagtcggt ttggcggaag tccagccgat tcaagggctt cccaggtggt
     1201 ttcaatcaac acccgatgtt gcgggtcaat gcaggcggct tcgcgaggag aaatgttgaa
     1261 aaacagcggg tcaaattcat caaagtgatc aacaaagctg ccaaaccggc aattggattt
     1321 tccaggtgcc gccggatccg ggtcataaaa cgcattgaca tcccagcgac tgggcggaat
     1381 ttcagtcacg gcatctacgc catttttcaa gagatcccaa aacgcttcgg gtgaactggc
     1441 atggccgcca aaccgacagc cgataccgac aatggcaatc gcttcagccg tttctgattt
     1501 ttgtggtggg tgaaaggtca gattttcaag cgtggccaga tggtttgaaa cggcatcaat
     1561 ggtcgggtaa tcccagacca gcgtcgggga aatcgggatg ccgagcactt tttcaaggtc
     1621 tccggccagg ttcaccgcat caatcgagga caggccataa aacgcaaatg gttgccggac
     1681 atcaacttcc gtggctggga ttttagccag ttgggccacc tgttcgacca gccaccgggc
     1741 aatagtatgc accgtacccg gatgcgcttt gagcgttggg gtgactggca gttcagccgc
     1801 gggtgccgga atcgagaccg gttcaaacgc cgcctgccac agcccgccat ccacaatttc
     1861 aagttcattg gccagaaagg cttttttgca ggcgtggcgg cggattttgc cgctcgacgt
     1921 tttggcaatg ctgcctggtt tgatgatggc aacggcgtgg cattgcaatt cgtgttcttt
     1981 ggccacggcc tgtcgaatag ccgtaatcac ttcattcaaa tcaggttttt gacggaactc
     2041 cagttcctgg acaatcacca gtcgttcgac cccaccaatt tcaaccgaca ccgccgcccc
     2101 gctgattgag cgcaacgagc gatggctgcg ttcgacggtc aattcaatgt cctgcggata
     2161 gtaattctgc ccatgaatga tgatcagatc cttaatccgg ccagtgacat agagttcgcc
     2221 agcgtccaaa aaaccaaggt cgccggtgcg taaaaacgga ccttcgccgg tatcggccag
     2281 aaaagcctga aaggtagacc gggtttcttc gggacgattc cagtagccac gggccacggt
     2341 ttttccggcg acccacactt cgccaacctg caggtcctgg caccgggtca gggtgtcggg
     2401 gttggcaatc acgatttgtt gttcaaacaa attgactccg catccaacca gggatcgtgt
     2461 ctggtctgtt tcgtgctcgg cagcctcgat ccggttatat ttaaatggtt cagacagaat
     2521 ggtttttacg gctggcccgg tgggttttcg accacctgac acaatgagcg tcgcttcggc
     2581 cagaccataa caggggtgca tggcttcggt gtggaatcca caggatctga agctttcagc
     2641 aaagcgattg acggtttctg accggatagt ttcagcgccg ttataggcca cttcccagca
     2701 gctcaaatca agtcctgctt tttcctcttc agtaattttg cgggcacaga ggtcgtaggc
     2761 aaaatcaggt gcgccgctga caaacccacg atatttgcta atcgctttca gccagcggag
     2821 cggtttttgc agaaatgcgg ttggggccat caagacgccg tgaaacccgg tatacaccgc
     2881 ctgcaacata ccaatcaggc ccatgtcgtg atagggcgga agccacgaca cacccggacg
     2941 ttcgggcgcg cattccatac accgtcgcat ggcttcttcg ttttgcataa tgttggcgtg
     3001 actgaccatc acaccacgcg ggataccagt cgaacccgac gtatattgaa gaaacgcaag
     3061 atcttcaggc gaaagagaaa ctggtttcca ttcattttcc cagccggctt cgatgctgtc
     3121 ggttgccacc caggccagtt tttgcaggtc aggggtgtga accatcatgg tctgggtttt
     3181 ggccagtaac gcggcgctgg tcaaggccac gctggcctcg gcatcagcca caatggactg
     3241 aatccgcgaa agcccgcgat tggggcgtgg cggataggct gggacagccg tcattcccgc
     3301 atacaggcag ccaaaaaagg ccgtcacata ttccagccct ggattaaaca gaagcatcac
     3361 gcgtgcgtca cgcggggcca cgctctggag tttggccgca atcgcacggg cctggcgatc
     3421 catttcaagg taagtgaggg tggcttcttc ggattctccg tccaccaaaa aggtgtaggc
     3481 cagacggtcg ggttggtgcc gggcacgaaa ctgcaaaact tcgactaaat ttgcatgcga
     3541 atgcaggggg gtggaaaaag gttttggtac aggcgtcatt gtggtgatgt gcaactcgtc
     3601 gggatgagac gcaagagatt gcaggagcgt atgaaattga tcgaataggt gagggatcga
     3661 agcgccctcg aagaggtcgg tattgtactc caaaattgcc accagcgcat catcaacctc
     3721 ggccatcatc aaatcgagat caaactgagc aatccgacgc ggtactggcc aggctgtcat
     3781 ctctaatcca ccaagacaaa taccagcttt tccgccaagt cccactgaga gcaagtctgc
     3841 caggtgtggt aaatggactt tttgcagcgt gaacatggtt tggaagattg gggaccggct
     3901 gaggtcgcgt tcaagctgga gtcgttcgac cagcaaggcc agtgggaaat cctggtgatc
     3961 aagtccctga accaccgtct ggcgaacacg ctccaggaat tcaagaaaca tcggatcccc
     4021 ggcacaattg acccggagcg gcaccggatt gaccagatag ccaaccaatc ctgtgagttc
     4081 cggctggttc cgtccagcca tcggtgaccc aacaatcaca tcttcctgtc tggtcagttg
     4141 atgtaggaac acaaaatagg ccgacagcaa cgtgacataa agcgtggtgt ggttggccag
     4201 actgagggct ttgagttgcc gggtggtttc ttggtcaatt tgaaaaacct gggtggcgcc
     4261 aatcatccgc tggacactgg ttcgggggtg gtcagttggc aggttgagaa ctggcaatgg
     4321 cagggcaagc gtggttttcc agaaattcca gagcctgtca ccttcgggtg aatcgagcat
     4381 cgccatctgg gccagggcat gctgaatcgg tgtgctggct ggcgctggca attgaatggt
     4441 ttttccctga gtggcggcct gatagatctg ggcaaattca tcgagcagca ccgccagcga
     4501 ccacacatca gcgataatgt gatggagcgt cagaagcaaa atatgatcgt ctgtctggcg
     4561 tgaaaacagc gtgacccgga gcagtgattc ccgcgataga tcaaatgggc gatgcgcttc
     4621 gtccagaagc gcctgttgaa gttgttcatc actccactgg cctgccgcca cggcatggaa
     4681 cgagacgttc atcgtggagt gaacctgctg tgagggcttt cctttcacca ttggaaaggt
     4741 ggtgcggagt gcttcgtggc gatcaaccag atattgaaat gcctgctcaa acgccgacac
     4801 atttaccggg cttccaatcc gaaccgcatt cacgatgtga taggccgtgc tttttggctc
     4861 cagcaactgc ataaaccaca gcgaacgctg gttgtaggag agaactcctg attgggggct
     4921 gaagacatcg ggctgaagac tcgcaagctc ggggctgaag atattgggct gaagacattg
     4981 ggctgaagaa gtcaggctag gggctgggag cttttcaaac tcggaggtga gaaacgccgc
     5041 cagcttttca atcgtcgggc gaaggaagat gtctcctggt gtcagtttca ggccaaattg
     5101 ttcctgcagc gtccgaatca gctccaacgc aatcagcgaa tcaatcccga gttcaaccag
     5161 actccgatca accggaatgt gctctgggtg agtttccagg acttcggcga gctggtggcg
     5221 gaggaaacct gtgaaaccca gggttccggg ttccgggttc cgggttccgg gttccacggt
     5281 gggagagaga cgtagttgct caacgggtgc aacctttaaa ttttccagat gacttaaatc
     5341 agtgccggaa cccgaaaccc ggaacccgga acccggttct tcaatgccgg aacccggaac
     5401 ccggaacccg
//
LOCUS       DAOQDO010000090         5385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9650_length_5385_cov_2.387617, whole genome shotgun sequence.
ACCESSION   DAOQDO010000090 DAOQDO010000000
VERSION     DAOQDO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9650_length_5385_cov_2.387617"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKZ53_02330"
     CDS             complement(<1..654)
                     /locus_tag="PKZ53_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39299.1"
                     /translation="MWTFLKNIRKLVMGGLLCLIFGAGISFPGLSQPLANNLNSAAEN
                     AHPTLKENKVEMARQYSLTKFYPISSYPGKIVTIFGTGFLFGTQVYFGGSRLIPSPNV
                     EFVDSNTLKVTVPASSTGSGNINGYLTVVIPGASQEFTTQNFPLPAAYPNAGDLTTIS
                     PEFVLWGDVSGDGLPAQANDITLARSFIQSQATPTLRQFVASDVYPCNCPLNIEGCSQ
                     "
     gene            complement(762..3200)
                     /locus_tag="PKZ53_02335"
     CDS             complement(762..3200)
                     /locus_tag="PKZ53_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNJ39300.1"
                     /translation="MRFENNTPYRHLRQWLGLGLAGLILLPLTPATALAQKEKETAPK
                     PAFALTIDNIMRGPDLVGTTPTQVRWSRDSQKVYFSWKEPGEPREKDPDTFMVNRDGS
                     NLQRLSEADARNAPPITADLSKDKAFSVFDRYGDLFLYNHTAGRAMQLTKTTEVESNP
                     HFTTDQKSIFFTRNDNLFLLSLETGALQQLSNFRSRPGPDEKEKGTDSQEYLKQEEKA
                     LIEILRQRAKKREEREAKAKERLPLKPFTIPGNLSLQSLELSPDQKYILASFTEEASR
                     SKIASVPFFVTESAYTEMVPTRNNVGDLQTRYKMAVVTLATGDVKFVEHSLKTTKKPK
                     PEAGKEPAGEPQTIDREVYYGSVVWSDDGSKAALLLRAFDNKDQWVTTLDPAAAKINI
                     LHTIHDDAWVDGPGVSTLGWLPDNQHLYFEWERDGYAHLYTLPTAGGQPKQLTSGQFE
                     VDNVQISEDKTRWYFTSNEVHPGEIHFYSMPLEGGTRTKLSTLPGSNLASVSPDGTML
                     ALIRSYTNRPPEVYLQPNQVGAEAKQVTTSPTAEWLSFPWIDPPVVTFQARDRATVYA
                     RLYKPKTAVKGGPAVIFVHGAGYLQNAHKYWSYYYREFMFHHLLMEKGYTVLDVDYRA
                     SAGYGRDWRTGIYRHMGGKDLDDQVDAARWLVSAHGVDAKRIGLYGGSYGGFITLMAL
                     FTQPDVFVSGAALRPVTDWAHYNHEYTSNILNSPQDDPEAYRRSSPIYFAEGLKGNLL
                     ICHGMVDLNVHFQDSVRLAQRLIELRKENWELAVYPVEDHSFVESTSWADEYKRIFKL
                     FEQTLKPVSKPVSR"
     assembly_gap    3388..3487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3788..>5385
                     /locus_tag="PKZ53_02340"
     CDS             3788..>5385
                     /locus_tag="PKZ53_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39301.1"
                     /translation="MQHRKFFHVIFFLTCFFLAFFVSMQRGCQHIQSQQVVPEFSHHI
                     PATEPPHAIIWPVAVEPAPENRLIGVPAADEELKTDDGTVESGVVMDDLIVVNRLTPV
                     SYPATLQRVRIRWDEFQNLPSPLGKSVQLLVFTDSTGSGHPVSPVTLSVNQSVTISQT
                     GTFLDFSINPVTIGSGDVYVGFQAPNPAQGVGFSADTNSPPQQRAFYSTNDGSTFQGP
                     LQFQDGTRPNILLRAVVSTSTGSTEELKTDDGTVDGRGFLENKMMMVNRLTPGHFPAT
                     LETIRIYFVSFQGQPSPVGGSGRLVVYTDPQGTGIPPVNAQPVISQSFTISRTGSFED
                     FAVPAVTLPSGDFYVGYQAGNPANGVGFAADSNGLAQLRSFYSQNDGVNFQGPLRFSD
                     GATANLMIRARVRYGGSTDTQPPSVNVVFPNGGETLSVGGTTQIRWTSSDNTGVSRHE
                     VWLSTSGGASYPTALATGLAGTAQAYQWFIPNTPTLSARVKVVAVDAAGNSGMDTSNG
                     NFTISSGGDTQPPTVRVLSPNGGE"
BASE COUNT         1296 a         1283 c         1360 g         1346 t
ORIGIN      
        1 ttgagaacag ccctcaatat tcaaaggaca attgcacggg tacacatcac tggcgacaaa
       61 ttgccgcagc gttggcgtcg cttgagattg gataaaagac cgtgccaatg taatatcatt
      121 tgcttgagcc ggcagcccat cgccggacac atctccccac aatacaaatt caggggatat
      181 ggttgtcaga tcgcccgcat ttggataggc agcaggaagc gggaaattct gggttgtaaa
      241 ctcttgggaa gctccaggga tgacgaccgt tagatatcca ttgatgtttc ctgagccagt
      301 tgaggaggct gggaccgtaa ctttcaatgt attgctatca acaaactcaa catttggtga
      361 tggaatgagc cggcttcccc caaaataaac ttgagtacca aaaagaaaac ccgttccgaa
      421 gatagtaact atttttccag gataagaaga gattggataa aatttggtta atgagtattg
      481 cctggccatc tccactttgt tttctttcag ggtggggtga gcattctcag ccgcagaatt
      541 gagattgtta gccaatggct gtgacaatcc tggaaaagaa atacctgctc caaaaatcaa
      601 acacaacaaa cctcccatga ctaattttcg aatgttttta agaaatgtcc acattgaaag
      661 ctccttattc ataaagaatt ggtttaggaa aagaaaggaa ccaaaaagtg cctttttggt
      721 tccctcctcg atatagaacc tgttgcagtt tacaacccag atcatcggct cactggtttg
      781 gaaacaggtt tcaacgtctg ttcaaagagt ttgaaaatcc gcttgtattc gtcagcccag
      841 cttgtgcttt cgacaaagct gtggtcttcg accgggtaga ccgcaagttc ccagttttct
      901 ttccggagtt cgatcagccg ttgggccagt cggaccgaat cctggaaatg aacgttgaga
      961 tccaccatgc cgtgacaaat aagcaaattc cccttcaagc cttcggcgaa ataaatcggg
     1021 gaacttcgcc gataggcttc gggatcatcc tggggtgaat tgagaatgtt tgacgtgtat
     1081 tcgtgattgt aatgtgccca atccgtcact gggcgtaacg cggcacctga tacaaacacg
     1141 tccggctgag taaaaagcgc catcaacgta atgaacccac cataactgcc accatagagg
     1201 ccaattcgtt tggcatccac gccgtgagca gaaacgagcc agcgggcggc atccacctga
     1261 tcgtcaaggt cttttccacc catgtgccga tagatgccgg ttcgccagtc acgtccgtac
     1321 ccagccgaag cccgataatc cacatccagc accgtgtacc ctttttccat caacaaatga
     1381 tggaacataa attcacgata gtaataggac cagtatttgt gggcattctg caaatatccg
     1441 gccccgtgaa cgaaaatcac tgccggacca cctttgacag cagtttttgg tttatacaaa
     1501 cgggcataca ccgtggcacg gtctctggct tgaaatgtga caactggtgg gtcaatccag
     1561 gggaaggaaa gccattcagc cgtcggtgaa gtcgttacct gtttggcttc ggcaccaacc
     1621 tgattgggtt ggagatacac ttccggcggg cggttggtat aggaccgaat cagcgccagc
     1681 atcgtgccat ctggcgaaac cgatgccaga tttgagccag gcaaggtgga aagtttggtc
     1741 cgggtgccac cttcaagcgg catcgaataa aagtggattt ccccaggatg gacttcattt
     1801 gaggtgaaat accatctggt tttgtcttcc gaaatctgga cgttatccac ttcaaactgg
     1861 ccgctggtta attgtttggg ttgtccgcca gcggttggaa gcgtgtagag atgagcatat
     1921 ccatcgcgtt cccattcaaa gtacaggtgc tgattgtcag ggagccagcc aagggttgat
     1981 acgcctgggc catccaccca ggcatcatcg tggatggtgt ggagaatatt gatttttgcg
     2041 gctgccgggt caagtgtcgt gacccactgg tccttattgt caaaagcacg caggagcaat
     2101 gcggcttttg atccatcatc tgaccagacg actgatccat aatagacttc acgatcaatg
     2161 gtttgtggct cgccagccgg ttctttcccg gcttcgggtt ttggcttttt ggtggttttt
     2221 aaactgtgct cgacaaactt cacatcgccg gtggcgagtg tcaccacggc cattttgtag
     2281 cgggtttgaa ggtctccgac attgttccgg gttggcacca tttccgtata ggccgactcc
     2341 gttacaaaaa acggaacact ggcaattttc gaccgggagg cttcttcggt aaagctggcc
     2401 agaatgtact tttgatcagg tgaaagttcg aggctctgga gcgaaaggtt gcccggaatg
     2461 gtaaacggct tgagcggaag tcgttctttg gctttggctt cgcgttcttc gcgttttttg
     2521 gcccgttgac gaagaatttc aatcagagcc ttttcttctt gctttaaata ctcctgactg
     2581 tccgtccctt tttccttttc atcggggccg ggcctggaac gaaagtttga aagctgctga
     2641 agggcgccag tttcaagcga aagcagaaac aggttgtcat tgcgggtaaa gaaaatgctc
     2701 ttttgatcag tcgtaaagtg tggatttgac tcaacttcag tggttttggt caattgcatt
     2761 gcccgcccgg cagtgtggtt atagagaaaa aggtcgccat agcggtcaaa aaccgaaaac
     2821 gctttatctt tagaaaggtc tgccgtgatg ggtggcgcat ttctggcatc agcttcgctc
     2881 agtcgttgca gattgctccc atcgcgattg accataaagg tatccgggtc cttttctcgc
     2941 ggttcacccg gttctttcca actgaaataa actttctggc tgtcacgtga ccagcgaacc
     3001 tgggttggcg tcgttcctac cagatccggt ccgcgcatga tgttgtcaat cgtcaaggca
     3061 aaggctggtt taggggcagt ttccttttcc ttttgggcca gggcagttgc tggcgtcagc
     3121 ggaagcaata tcaagccggc cagtccgagc cctaaccatt ggcgaagatg gcgatacggt
     3181 gtgttgtttt caaaacgcaa tgggtttccc tctcttcttg tatgaaatgg acaatctgaa
     3241 atgtgtgcga cggatagatt ggaatgatga aggatgaact atgaactatg aagagccatt
     3301 gtgcgaagtc agtctttgca tttgagcatg aaacaggtca atttttgaga atttgaagcc
     3361 tcactgccct ctgggcgtgg attatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnaca ggtcaatttt tgagaatttg aagcctcact gccctctggg cgtggattat
     3541 ttcccacaat gaggaaaaag agccagatgg aaaaacagtg agcaggaaca agcaggggta
     3601 cagacagaga aactccggga tagcagagac aaacggcaga agctttaaaa taaaggattt
     3661 agggctagat gtcttgtcag gctgccaccc taacacccta tcactctgtc accgtgtccc
     3721 cagccaattg cggaacgagg tttgccctta ccttgagaga acgccttctc tgaaggaggg
     3781 gaaagctatg caacacagaa agttctttca tgtgatcttt ttcttgactt gctttttcct
     3841 ggctttcttt gtttcgatgc aaagaggttg ccagcacatc cagtcacagc aggtggttcc
     3901 tgagttttct catcatatac cagccacgga accaccgcac gccataatct ggccggttgc
     3961 ggttgaaccg gcacctgaaa accgcctgat cggtgtgcct gcggctgacg aggaattaaa
     4021 aaccgacgat ggaacggttg aatcaggtgt ggtgatggat gacctgatag tggtcaaccg
     4081 gttgacccca gtttcatatc cagccacgct gcaacgagtg cggattcgat gggatgagtt
     4141 ccaaaatctg ccctcaccgc ttgggaaatc agtgcagtta ctggtgttta cagattcgac
     4201 cggaagtggt catccggttt caccggttac tttgagtgtc aatcagtcgg tgacaatttc
     4261 tcaaaccggt acgtttctcg atttttcaat caatccagtt acgattggat caggtgatgt
     4321 ttacgtcgga tttcaagcac cgaacccggc tcaaggagtt gggttttcag ccgacaccaa
     4381 tagtccgcct caacaacggg cattttattc aaccaatgac ggaagcacct ttcagggacc
     4441 gttacagttt caggacggaa cacgacccaa tattctgtta cgggcggttg tttccacatc
     4501 aaccgggagc accgaagaat taaaaaccga tgacggtaca gttgatggtc gtgggtttct
     4561 tgaaaacaaa atgatgatgg tcaatcggct cacgccaggg cactttccgg ccacgctgga
     4621 aaccattcga atctactttg tttcatttca aggccagccc agtccggttg gcggttcggg
     4681 acgactggtg gtctatactg atccacaagg caccggaatc cctccggtca atgcgcaacc
     4741 cgtgatcagt caatcattta ccatttcccg tactgggtcg tttgaggatt ttgcggtacc
     4801 tgccgttacc ttgccgtccg gtgatttcta tgttgggtat caggctggga atcctgccaa
     4861 cggcgtaggg tttgcggctg actcaaatgg cctggcccag cttcggagtt tttattccca
     4921 aaatgacggg gtcaatttcc agggaccact tcggttttct gatggcgcaa ccgccaattt
     4981 gatgattcgg gcccgagtca ggtacggagg ttccaccgac acccagccac cctcggtcaa
     5041 tgtggtgttc ccaaatggcg gcgaaacgct ttcggttggt ggcacaactc aaattcgctg
     5101 gacctcttcg gacaataccg gggtgagccg acacgaagtg tggctctcta ccagcggcgg
     5161 cgccagttac ccaaccgcac tggcaacggg tctggccgga accgcccagg cttatcagtg
     5221 gttcatcccg aacaccccaa cgctgtcagc ccgagtgaaa gtggtggccg ttgatgcagc
     5281 gggaaacagc ggaatggata cgtcaaacgg gaatttcacc atttcaagtg gcggtgatac
     5341 gcaaccacca actgtccgag tcctgtcacc caacggaggc gaaca
//
LOCUS       DAOQDO010000091         5375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9676_length_5375_cov_3.323120, whole genome shotgun sequence.
ACCESSION   DAOQDO010000091 DAOQDO010000000
VERSION     DAOQDO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9676_length_5375_cov_3.323120"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..718
                     /locus_tag="PKZ53_02345"
     CDS             218..718
                     /locus_tag="PKZ53_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNJ39302.1"
                     /translation="MGFAKTLWIILRRLLMTLVFAVVFITSAVMTYYFSRGEEVAVPD
                     VVGKNEDQARKSAAQIGMKIEVIDVFDSQEQPGKVLRQYPKPGAIIRKGGSTTLRINV
                     SRVKQTSRNTDSFPLTPPTQPPFDLTPWSEDLCGVLIAFRLPSDQSQPERLRKSVSPS
                     HLVCQL"
     gene            715..1401
                     /gene="rpe"
                     /locus_tag="PKZ53_02350"
     CDS             715..1401
                     /gene="rpe"
                     /locus_tag="PKZ53_02350"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNJ39303.1"
                     /translation="MIPEIKIAPSILSADFARLGEQVQLVEAAGAQLLHVDVMDGHYV
                     PNITIGPLVVEALRRVTDMPLDTHLMISDADRYIDAFARAGSTSISVHVEAVTHLHRT
                     LEAIRKLGAQPGIAINPGTSLALLDEVLPYADFILIMTVNPGFGGQKFIPTSLQKIAR
                     LRQMITERKLNVRIEVDGGIGPNNVRSLVEHGAEWIVAGSAIFGAPDIGAAVQAMQAA
                     AEGLATANIS"
     gene            1653..2783
                     /gene="bamD"
                     /locus_tag="PKZ53_02355"
     CDS             1653..2783
                     /gene="bamD"
                     /locus_tag="PKZ53_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNJ39304.1"
                     /translation="MAFKRILIGFCIGLLALQAIGCGDKAAKKVESEAPRDGRDREIY
                     VDGLKAIQKRRYEEGRILLNTMISTYDNSPILPLAKLLIADSFYREGGSSNLAQAEVE
                     YREWLQFFPNHQLADDVLLKIADIHIRQVDAPNRDWTEARAAERELVRIQKEYPQTDL
                     NPEVKDKLKFVREQLGMHELGITRFYFKYRQGWNAVVSRGRDMLKKYPEFSYVDEVLF
                     LVGSALVEKEDTPEAAKYFSRLVREFPNSEYRDKSAEFLERFGMEVPDPSPTAETKET
                     LVERKGFISRKKIEIFGPNYDINKDGVVLNKDEQLKPEIEAMFNLPKTDLTTPDATLT
                     TAGGKVKTFNKDDEKQKQDGTQKSSENPAIKQSTEKKSRGVQ"
     gene            2896..3642
                     /locus_tag="PKZ53_02360"
     CDS             2896..3642
                     /locus_tag="PKZ53_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNJ39305.1"
                     /translation="MRTSNPTLNQRAFQNPGYGAVYGQTMTIDGTVNKTGFLLILLTL
                     SAAVPWTLFFAFKAIPLVMLLALIGLIGGLVTGLVTVFKKEWSMVTAPLYACFEGLLL
                     GSISAIFERSYPGIAIQAVGLTFGTLFGLLLAYRSGMIRVTENFKMGVIAATFGICLM
                     YLVSFVLSLFGIRLGFIHGNGLLSIGISLVIVGVAALNLVLDFDFIENAARQGAPKYM
                     EWYSAYGLLVTLVWLYLEILRLLSKLRSRD"
     gene            3840..>5375
                     /locus_tag="PKZ53_02365"
     CDS             3840..>5375
                     /locus_tag="PKZ53_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNJ39306.1"
                     /translation="MKKRLFLFAACFLSLFAGPATVAPVLAQTPTPQLATSPASFDFY
                     ARGPYREEFPRPSKILGYEPGEFHTNYASFERVLREYAGKSDRLKVIERERTAERRTM
                     YLLAISSPENMARLDQIKANVAKLADPRTLSEADAKKLAAETPIVVWLSYNIHGNESA
                     GFEAAMHTLYQLVASQDPKIVEALKNTVVLINPAQNPDGHERFATWYNAVGIGRAEPF
                     AMEHDEPWSVYGRNNHYLFDLNRDLIASSQIESQSSMKAFLEWHPQISADHHGQTANF
                     FFPPAALPINPNLPKETTNKWLEVIGRGNASAFDRYGWNYYVRDVFDLFYPGYWDSWP
                     ALNGATGMTYETDGGGWKGLNWRRDDGTNVTFRDGIARHVTASLATIETAAANREARL
                     FDYYQFFKTGIEEGTKGKIRQFVMLPGKDPGRTSKLVANLLRNGIEVQQAGESFSAKG
                     VHDYFGSAAGNREFPAGSYIVTLNQPQTRIAKALLEHHTPQDDEFLKRQKEKRERNER
                     RGTS"
BASE COUNT         1358 a         1420 c         1318 g         1279 t
ORIGIN      
        1 gttgaaagcc tgcctggaac cgtgtacact tgctgcccca tctgggaaca actattggct
       61 gacctgggat ttaccacaat gccacgccct caccacctca tcaggtggct ttcgatgtgt
      121 ggcatttctg aaaacccgaa catccttttt ctgattcacg accgggctgt gcctgtgtga
      181 ccacatacag gtgccacctg gatcgcaccg agaaaacatg ggttttgcaa aaacactttg
      241 gattattctc cgacgactgt tgatgaccct ggtctttgca gttgtgttta ttaccagtgc
      301 ggtcatgacg tactacttct cccgtggtga agaagtggcg gtccccgacg tggtaggaaa
      361 aaacgaagat caggctcgga aaagtgcggc ccagatcggg atgaaaattg aagtcattga
      421 tgtctttgat tcgcaggaac aacccggcaa agtgctccgc cagtacccca aacccggtgc
      481 catcatccgc aaaggtgggt ctaccaccct gagaatcaac gtcagccggg tgaaacaaac
      541 atcgcgcaac acagactcat tcccgctcac acctcccact caaccaccct ttgatttgac
      601 tccgtggagc gaagacctct gtggcgtcct tattgcgttt cggctgccat ctgatcagag
      661 tcagcctgaa cgccttcgaa aatcggtttc gccttcccat ttggtgtgcc agttatgatc
      721 cctgaaatta aaattgctcc ttcgattttg tcggctgatt ttgcccggct tggcgaacag
      781 gtccagctcg ttgaggcagc aggtgcccag ctcctgcacg tagatgtgat ggacggccac
      841 tatgtcccca acatcacgat tggcccgctg gtggttgaag cccttcgtcg tgttacagat
      901 atgccgctcg atacccattt gatgatctcc gacgcagatc gctatattga tgcttttgcc
      961 cgggctggat ccacttcgat ttccgtccat gtcgaagccg tcacccattt gcatcggaca
     1021 cttgaagcca tccgcaagct cggtgctcag ccgggcattg cgatcaaccc cgggacctcg
     1081 ctggctttgc tggatgaagt ccttccgtat gctgacttta tcctgatcat gacggtcaat
     1141 ccaggatttg gcggtcaaaa atttatcccc acttcgcttc aaaaaattgc tcggttgcgt
     1201 caaatgatca ccgagcgcaa attaaatgtt cgcatcgaag ttgatggcgg tattggtcca
     1261 aacaacgtcc ggtcgctggt cgaacacggc gccgaatgga tcgttgctgg ctcagctatt
     1321 tttggggctc cggacatcgg agctgccgtt caagcaatgc aggcagccgc cgaagggctg
     1381 gccactgcaa atatttcata gcgcttcgtt tttcggttca gacccagaag gtcacatatc
     1441 gcgcgaccag actcatcccc tcactcaatt tcagtgaact cctccggtac gctcgatctg
     1501 gtttgttttt gtagccaatc ggcaccaatc gcacttgtca gaaaaaataa cattatccat
     1561 ctgtaacgtg ttgactccaa aaacaattat atatagctct cttgaccaac cgactgcccc
     1621 gaagacgaca aacattttca caaaggaagt ttatggcatt caaacggata ttgattggat
     1681 tttgcattgg tctgctggcg ctgcaggcaa ttggttgcgg cgacaaggcg gccaaaaagg
     1741 ttgaaagcga agccccacgc gatggacgtg accgggaaat ttacgttgat ggactcaagg
     1801 cgattcaaaa gcgacgctat gaagaaggcc gtatcctgct caacaccatg atcagtacct
     1861 atgacaacag cccgattttg ccgctggcca agctcctgat cgccgattcg ttttatcgtg
     1921 aaggcggttc atccaatctg gctcaggccg aagtcgaata tcgtgaatgg ctgcagtttt
     1981 tcccaaacca tcaactcgcc gacgatgtgc tcttaaaaat cgccgatatt cacatccgtc
     2041 aggttgatgc cccaaaccgg gattggaccg aagcccgtgc tgccgaacgc gaactggtac
     2101 gaattcaaaa ggaatacccc caaaccgatc tcaaccccga agtcaaagac aagctcaaat
     2161 ttgtccgtga gcaactcggc atgcacgaac tgggcatcac ccggttttac ttcaagtacc
     2221 gtcaggggtg gaatgcggtt gtttcgcgtg gccgggatat gttgaaaaaa tacccggaat
     2281 tttcctacgt tgacgaagta ctcttcctgg tcggttcggc cctggttgaa aaggaagata
     2341 cccccgaagc agccaagtat ttctcacgcc tggtgcgtga atttcccaac agcgaatatc
     2401 gggataaatc ggctgaattc ctggaacggt ttggaatgga agttcccgat ccaagcccga
     2461 cggctgaaac caaggaaacc ctggttgagc gcaaaggttt tatttcacga aaaaagatcg
     2521 agatttttgg tccaaactac gacatcaaca aagatggcgt ggtactcaac aaagatgagc
     2581 aactgaaacc cgaaatcgaa gccatgttta acctgccgaa aactgacctg acaacgccgg
     2641 atgccacgct gaccacggct ggcggcaagg tcaaaacctt taataaagac gacgaaaagc
     2701 aaaagcagga tggaacacag aagtcttcgg aaaatccggc aatcaaacaa agcaccgaga
     2761 aaaagagccg gggcgtacaa taaaacggac aggaactgtt tgttgcacag acccaggcaa
     2821 atagcagaca atcaagggag caacggagat aatctccggg ctcccttttc ttttttattt
     2881 tcaggaggtt gactgatgcg aacatcgaat ccgactctca atcaacgagc gttccagaat
     2941 cctggctacg gtgcggtcta tggtcagact atgacgattg acggaacggt caataaaact
     3001 ggttttttat tgattttact gacgttatcg gcagccgttc cctggacgct gttttttgca
     3061 ttcaaagcaa ttccactggt gatgctgctc gccctgattg gactgatcgg aggactcgtc
     3121 accggactgg ttacggtttt caaaaaagaa tggtcaatgg ttacggcccc gctctatgcc
     3181 tgttttgaag gtctgttgct tggctcaatc tcagccatct ttgagcgatc ctatcctggc
     3241 attgccatcc aggcggtggg gctcaccttt ggcacactct ttggtttgtt gcttgcctac
     3301 cgttcaggaa tgatcagggt taccgaaaat ttcaaaatgg gtgtgattgc ggcgaccttt
     3361 ggtatttgtc tgatgtacct ggtgagcttt gttctcagtt tgtttggcat caggcttgga
     3421 ttcattcatg gaaacggtct gttgagtatt ggaatcagcc tggtgattgt cggtgtggcg
     3481 gccctgaatc tggtcctgga ctttgatttt attgaaaatg cagcccgcca gggtgccccc
     3541 aaatacatgg agtggtattc agcttatgga ctgctcgtga cgcttgtctg gctgtacctg
     3601 gaaatcctgc gcctgctctc caagctccgt agccgagact aacgaacatc aaaatgacag
     3661 ggtgacaaca taggaataag cgccaaggtc aagaaacaaa ctctcatttt gccactctgt
     3721 catcttgtcc ccctgtcatt ttggttgtca gaaatctatg gttatgtacc ataggaatca
     3781 ggtcaaacaa ctccacacat ctcaattcaa tcaggctgca cctcacttca actaaaacta
     3841 tgaagaaacg tcttttcctg tttgctgcgt gttttttatc gctctttgcc ggtccagcaa
     3901 ctgttgcccc agtactggct caaacgccaa ccccacaact ggcaaccagc cccgccagct
     3961 ttgattttta tgcgcgtggt ccctaccggg aagaattccc ccgtccgtca aaaattctgg
     4021 gatacgaacc cggtgagttt cacaccaact atgccagctt tgagcgcgtg ctgcgtgagt
     4081 atgccggcaa gtcagaccga ttgaaggtga tcgagcgcga acgcacagcg gaacgccgga
     4141 cgatgtacct gctggccatc agttctcccg agaatatggc ccgtctcgac cagatcaaag
     4201 ccaatgttgc caaactggct gacccgcgaa ctctctctga agctgatgcc aaaaaactcg
     4261 cggcggaaac accgattgtg gtctggttga gctataacat ccacggcaat gaatcagccg
     4321 ggtttgaagc cgccatgcac acgttgtatc aactggttgc gagccaggac ccaaaaattg
     4381 tcgaagcctt gaaaaacacg gttgtgctca tcaatccggc ccaaaatcct gatggtcacg
     4441 aacgatttgc cacctggtac aacgcggttg gaattgggcg ggcagagcca tttgcaatgg
     4501 agcacgacga gccgtggagc gtgtatggac gcaacaacca ctatctgttt gacctgaacc
     4561 gtgacctgat tgccagttct caaatcgaat cacaatcttc gatgaaagcg tttctggagt
     4621 ggcatccaca gatttcagcc gaccaccacg gacaaaccgc caatttcttt ttcccgccag
     4681 cggcactccc catcaaccca aacttgccaa aggaaaccac caacaaatgg ctggaggtga
     4741 tcggacgtgg caatgcgtcg gcctttgacc gatatggatg gaattattat gtccgtgacg
     4801 tgtttgatct gttttatcca gggtactggg atagctggcc ggcattaaac ggtgcaaccg
     4861 gaatgaccta cgaaactgat ggcggtgggt ggaaaggact caactggcga cgtgatgacg
     4921 gcaccaacgt cacgtttcga gacggaattg cccgccacgt caccgccagc ctcgccacca
     4981 ttgaaacggc ggcggccaat cgcgaagccc ggctctttga ttactaccag tttttcaaaa
     5041 ccgggattga agagggaacg aaaggcaaaa ttcgtcaatt tgtgatgttg cccgggaaag
     5101 atccgggccg gaccagcaaa ctggtggcca atctgcttcg caacgggatt gaagttcagc
     5161 aagctggcga atcattttcc gccaaaggag tccatgatta ttttggatca gccgccggca
     5221 accgtgaatt cccagccgga agttacatcg tcacgctcaa tcaaccccaa acccgaattg
     5281 ccaaagcact cctggaacac cacacgccgc aagatgatga atttctcaaa cgccagaagg
     5341 aaaaacgcga acgcaatgaa cgtcgcggaa ccagt
//
LOCUS       DAOQDO010000092         5374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9680_length_5374_cov_3.574168, whole genome shotgun sequence.
ACCESSION   DAOQDO010000092 DAOQDO010000000
VERSION     DAOQDO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9680_length_5374_cov_3.574168"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PKZ53_02370"
     CDS             <1..1176
                     /locus_tag="PKZ53_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39307.1"
                     /translation="LNISIKPKVIDDLVQMTLSVESSGLVASTAESLTPTFTSRKVKS
                     VARVRDGQTSIAASVMRQDDSNSRRGIPILSLIPGVGNLFSTPTKSKNATYVLITVTP
                     HILRGGAVSEEDEKLTFGPLDFQIGPNGAQGYGRKLSLLELVDQADVKDQKEAEKGRQ
                     QAPSNQQNQSPYAPAKGTNQSKADFQGVKQGDGIVRQQTVPVGQTTIQNSNIQTYTPT
                     VLPNNETSIPQTNPNPNDGNSPSKENPAQPSAPVQLTPVISPSIRPSVGSIVSLAVIV
                     SSANVKISSAGFYIHYNSAVVKPISIRDGGLFNGPFDQRDDGSSLYAKTSLSPNLGSI
                     PAAGQLIVVDFQVIGTGPADIGFDMIEIFGADQQQVPVINNPIPTITAIGPRQGGQQ"
     gene            1335..1853
                     /locus_tag="PKZ53_02375"
     CDS             1335..1853
                     /locus_tag="PKZ53_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ39308.1"
                     /translation="MQDRHPSHFHLRQRASHLQNGTSLLELVITLAILAILTAAAVPM
                     TANTVKSRREVELRRALRELRDSIDRFNRIEYVLFQGQRIPIEDRTPSGFPKKLNILY
                     EGFIPAGQVNDKKVYHLRRLPVDPMTGTAEWGIRSSTDEPDDYSTNGDDVFDVYSLSE
                     GTALDGTKYKTW"
     gene            1959..2351
                     /locus_tag="PKZ53_02380"
     CDS             1959..2351
                     /locus_tag="PKZ53_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ39309.1"
                     /translation="MSPRSTRGFTLLELAITISLMLILAAIAVPVYQSLIHRAKETVL
                     HDTLFKLRDSIDKYTNHKEAAPATLQDLVTAKYLREVPIDPITGRNDTWTVKLEEEAS
                     SRDGKIGIRDVFSGAEGISSEGTPYAEW"
     gene            2445..3425
                     /locus_tag="PKZ53_02385"
     CDS             2445..3425
                     /locus_tag="PKZ53_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39310.1"
                     /translation="MNLLQRILKTFSAPNLPHYACAFASSHISMAQVLRHHGHRHIER
                     SSFLPVSSELFRPSFSGINIPNVSALADAVFSLGKGIGLGREKLWAVTLPDEVTRPHI
                     ITLHSSITNEPDVIEDMLVLRIERLVGESIDKLRVSYTPLSPVAGQKRFLVSVAVEAV
                     MREYERLFDELGWHVGLIWPTSLAEAVWLNRPNALPNQILLSSTPNEMTVLILHDQDP
                     VLLRTQTFEETEFGDTLLRLLLFYRDRAGEIIPGVTMADNPLDSTVFEVLALGDQSHT
                     KTIRDCIQEVFGASPVIVSSDTLGLPVANLMTDFAQIAAAAGLASLSLPA"
     gene            complement(3480..5207)
                     /locus_tag="PKZ53_02390"
     CDS             complement(3480..5207)
                     /locus_tag="PKZ53_02390"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNJ39311.1"
                     /translation="MKKLLALVCICLMVGSLVSESVLGAHRSQSPDVKKSVVLQALED
                     ELQRSFKILTQKGDPPPYFVSYTASDIHRVEVTASLGALQNSQNSHYRLLDVDVRVGG
                     YDLDSSHQIRGENAFFSSRVYSPASYLPVEDDPDAIKSALWLQTDKRYREAVEALIKI
                     KANRAIKVQEEDLSADFSKETPQVAVQPLVSFDVDVAAWEKKAREYSALFARYPEILQ
                     GAVTFYAEASNKYLVNSEGTSLQHGRLFGRLGFYAQTKAEDGMNLYRYEAFDTQSLDR
                     LPDEATVLRVIEKMSRDLLALRNAPVVEPYTGPAILSGRASGVFFHEIFGHRIEGHRQ
                     KDEDSGQTFTKRVNQSVLPGFLSVYDDPTLKALNNIDLNGFYDFDDEGVKAQRVPIVE
                     QGILKNFLLSRSPIQGFSRSNGHGRKSPGFAPVGRQGNLIVQTRKPVSEAKLREMLIS
                     ECKKQKKPFGLIFEDISGGFTFTQRDTPQAFQVTPIMVYRVYTDGRPDELVRGVDLIG
                     TPLTSFSKIIAGSDKVEVFNGMCGAESGWVPVSAISPSLLTSQIEVQKKEKSSERLPI
                     LPSPRDVTK"
BASE COUNT         1397 a         1433 c         1261 g         1283 t
ORIGIN      
        1 ctcaatattt cgatcaagcc caaggtcatt gacgatctgg ttcaaatgac cctcagcgtt
       61 gaatcctcag ggctggtagc ctcaacggca gaatcattaa ccccgacatt cacgtcacga
      121 aaggtaaaaa gtgtggcccg ggtccgtgat ggccaaacct ccattgcggc cagtgtgatg
      181 cgccaggatg actcaaactc caggcgcgga attccaatcc tgagtctgat tccaggagtc
      241 ggaaacctgt tttcaactcc gaccaaaagt aaaaatgcca cttatgtctt aattaccgtc
      301 acaccacaca tcctgcgtgg tggtgcggtg agcgaagaag atgagaagct gacctttggc
      361 ccacttgatt ttcagattgg tcccaatggt gctcaaggat atggccggaa gctgagcctg
      421 ctcgaactgg ttgatcaggc cgatgtgaaa gatcaaaaag aagccgaaaa aggtcgtcag
      481 caagcgccgt ccaatcagca aaatcaatct ccttacgctc cagcgaaagg caccaatcag
      541 tccaaagctg acttccaggg ggtgaaacag ggtgatggga tcgtccgtca gcaaacagtc
      601 ccggttggac aaaccacgat ccaaaactca aatattcaaa cctatactcc aaccgttctg
      661 cccaataacg agaccagcat cccgcaaacc aacccaaatc caaacgacgg aaattctcca
      721 tccaaagaaa atccggctca accatcagcc cccgtccaac tgaccccagt catcagtccg
      781 tcaattcgac catcagttgg gagcattgtc agcctggccg tcattgtcag ctcagcaaac
      841 gtcaaaattt catccgccgg gttttacatc cactacaact cagccgtcgt caaaccaatc
      901 agtattcggg atggagggtt gtttaatggg ccatttgacc agcgcgatga cggctcctcg
      961 ttgtatgcca aaacttcgct gagccccaac ctgggctcca tcccagccgc cggacaattg
     1021 atcgtggttg attttcaggt tattggtacc gggccagccg atatcgggtt tgatatgatc
     1081 gaaatttttg gtgctgacca gcaacaggtg ccagtcatca ataatccaat tccaacgatt
     1141 acagccattg gtccacgcca gggcggacag caataggatt tagcttttgg atctgggact
     1201 gaagcttttg ggttgaaaag acttcagtcc cagtcaaagc aagaatttgg aactggccga
     1261 ccaggacatc gtgagtagct tcagagggcg agccctcacc actctttcaa tcacacatcg
     1321 ctcagaaatc gaatatgcaa gatcgacacc cgtctcactt ccacctccgt caacgagcca
     1381 gtcaccttca gaatggcacc agtttgctgg aactggtgat taccctggcg attctggcaa
     1441 tcttgaccgc cgccgccgtc ccgatgacag ccaataccgt caaaagtcgt cgcgaagtcg
     1501 aattacgccg ggcactcaga gaacttcgag actcaattga ccgctttaac cgcatcgaat
     1561 acgtcttatt tcaaggacaa cgcattccga ttgaagatcg aacgccatct ggttttccca
     1621 aaaaactgaa tattctctat gaagggttca ttccagccgg gcaggtcaat gacaaaaaag
     1681 tgtatcacct gcgccgtctg ccagttgacc caatgacggg aactgccgaa tggggaatcc
     1741 gctcatcaac cgacgagcca gatgattatt cgaccaatgg ggatgatgtc tttgatgtct
     1801 attcgctttc agagggaacg gccctggatg ggacaaaata taaaacctgg tgagcctcct
     1861 cctggcaggg atgagacttt ggtcaacgtg taattttatt atttgggttt acaggattta
     1921 tgggtattca acggcatttc agacacaatc ttcgccagat gtcaccccgt agtacacgtg
     1981 ggtttacctt gcttgaactt gcaatcacta tctcactgat gctgattctg gctgcgattg
     2041 cagtaccggt ttaccagtca ctcattcatc gcgccaaaga aacggtattg cacgatacgc
     2101 tctttaaact ccgggacagc attgacaaat acaccaacca caaagaagcc gcaccagcca
     2161 cactccagga tctggtcaca gcgaaatacc ttcgggaagt ccccattgat ccaattactg
     2221 gacgcaatga cacctggacc gtgaagcttg aagaagaagc ctcttcacga gacgggaaga
     2281 ttggcattcg cgatgtgttt agcggggctg agggcatttc atctgaaggg accccgtatg
     2341 ctgagtggta aaatcgccat agatgttctg accagagttt acctgggcca gccaattggc
     2401 tggccttccc tatctctcca acttgatcaa cacaatacca accaatgaat ctgctacagc
     2461 gcatcctgaa aacatttagc gcaccaaatt tacctcatta tgcctgtgcc tttgcctcgt
     2521 ctcacatttc gatggctcag gttttacgac atcacgggca ccgccacatt gagcgaagct
     2581 cctttcttcc cgtctcttcg gaactgtttc gaccatcgtt tagcggaatc aatattccaa
     2641 atgtgtcggc actggccgac gcggtctttt cgcttggaaa aggaattggg ttaggccggg
     2701 aaaaactctg ggcagttacg cttccggatg aagtcacccg gccacatatc atcactttac
     2761 actccagtat caccaacgaa ccggatgtga ttgaagatat gctggtgctc cgtattgaac
     2821 gactggtcgg tgaatccatt gataagctga gagtttcgta tacgccgctt tcaccagttg
     2881 ccggacaaaa acggtttctg gtcagcgttg cggttgaagc agtaatgcgg gaatatgagc
     2941 ggttgtttga tgagttaggc tggcatgtcg gtctgatctg gccaacctca ctggctgagg
     3001 ccgtctggct gaaccgtccc aacgcgttgc caaaccaaat tctactcagc tcgacgccga
     3061 acgaaatgac ggtgttgatc ctgcacgacc aggatccggt gttgctccga acccaaacgt
     3121 ttgaggagac tgagtttggt gataccttgc tccgactgct cctgttttac cgggatcgcg
     3181 ccggagaaat catccctggt gtcaccatgg ctgacaatcc actggactct acggtgtttg
     3241 aagtcctggc tctgggtgac caatcgcata ccaaaaccat tcgggattgt attcaggaag
     3301 tttttggtgc ctcgccagtc attgtgtcct ctgacacgct tggcctgcct gttgccaatc
     3361 tgatgaccga ttttgcgcaa attgccgccg ccgccggcct ggcctctctc tcattgccag
     3421 cgtgatgaaa taacaaggtg acaccatgac caatgacaat ggtcgcgctg tcacctggat
     3481 cactttgtca cgtcacgagg tgaaggcaaa atcggcaacc gttcggaaga tttttccttc
     3541 ttttggactt caatttgaga ggttaaaaga ctgggtgaaa tggctgaaac tggaacccag
     3601 ccggattcag caccgcacat cccgttaaat acctcgactt tatcgctgcc agcaatgatt
     3661 ttgctaaatg aagtcagcgg cgtcccaatc aaatcaaccc cacgcaccag ttcatctggc
     3721 cgaccatcgg tatacacccg atacaccata attggcgtta cctgaaaagc ctgaggtgta
     3781 tcgcgctggg taaaagtaaa tccaccagaa atatcttcaa agatcagccc aaacggcttt
     3841 ttctgttttt tgcattcaga aatcaacatc tcgcggagtt ttgcttcaga aaccggcttg
     3901 cgggtctgca caatcaggtt cccctggcgt ccaaccggag caaaccctgg ggatttgcgt
     3961 ccatggccgt tagatcggga aaacccttga atcggagagc gtgacagcaa aaagtttttc
     4021 agaatcccct gctcgacaat cggcacccgc tgggctttga ccccttcatc atcaaagtca
     4081 taaaacccat tcaaatcaat attgttcaac gctttcaacg tcgggtcatc atagacggaa
     4141 agaaaacccg gcaggaccga ttgattaacc cgtttggtaa aggtctggcc gctgtcttca
     4201 tctttttggc gatgaccttc gatccgatga ccaaaaattt cgtggaaaaa gaccccactg
     4261 gcgcgtccag ataaaatggc cggcccggtg tacggttcaa ccacgggtgc attccgcaac
     4321 gccagcagat ccctggacat tttctcaatc acccggagaa cagtcgcttc atctggaagc
     4381 cgatccagcg attgcgtatc aaacgcctca taccggtaca aattcattcc gtcttcggct
     4441 tttgtttgcg cataaaatcc aagccgccca aaaagccgcc cgtgctgcag cgaagttcct
     4501 tcgctattga ccagatattt gtttgatgct tcggcataaa aagtcaccgc cccctgtaaa
     4561 atttcagggt atctggcaaa cagtgctgaa tactcacggg cttttttctc ccaggccgcc
     4621 acatctacgt caaatgacac caggggttga accgccacct gaggtgtttc ctttgaaaaa
     4681 tcagccgaaa ggtcttcttc ctgaacttta atcgcccgat tggctttgat cttgatcagg
     4741 gcttctacgg cctcgcgata ccgcttgtcc gtctgaagcc acagggcgct tttgatggca
     4801 tccgggtcat cttcaaccgg taaataactg gccggagaat atacccgtga tgaaaaaaag
     4861 gcgttttctc cccggatctg atggctgcta tcgaggtcat atccgccaac acggacatcc
     4921 acatccagca ggcggtagtg tgaattctgt gaattttgca gtgccccaag tgaggcagtg
     4981 acttcaaccc ggtgaatatc tgaggcggtg tagctcacaa agtacggtgg aggatcgcct
     5041 ttctgggtaa ggattttgaa ggaccgctga agttcgtctt ccaatgcctg gagcaccaca
     5101 ctttttttga catcgggtga ttgtgagcgg tgtgccccca gaactgattc agataccagg
     5161 ctgccaacca tcaggcaaat acaaaccaaa gccagtaatt ttttcatgtg aaattgtcag
     5221 aaaaactttc ttgggataga aacttatgga gctggatgga aatatcgtac caccagggtt
     5281 tagggttccg gaaaaggaca taaaggacat aaaggacata aaggacataa attcgaaaac
     5341 ccggaacccg gaacccggaa cccggaaccc ggaa
//
LOCUS       DAOQDO010000093         5356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9743_length_5356_cov_2.981702, whole genome shotgun sequence.
ACCESSION   DAOQDO010000093 DAOQDO010000000
VERSION     DAOQDO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9743_length_5356_cov_2.981702"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKZ53_02395"
     CDS             <1..663
                     /locus_tag="PKZ53_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ39312.1"
                     /translation="TEQEGKMLKHLFPKATLLMKADATEETIKTVHRPEVLHIATHGY
                     FYADPDQPGPDAPADTRSVTPRSDAEASEELRMQNPLTRSYLFFAGANRAGDTEKDGT
                     LTALEAAGLDLWGTKLVVLSACDTGLGEIRFGEGIYGLRRALVLAGSETQMMSLWPVA
                     DVATSKLMVAYYQRLKKAEGRSQALRNVQLNFLKDPKYRHPYYWASFFTSGEWANLAG
                     VR"
     gene            complement(812..973)
                     /locus_tag="PKZ53_02400"
     CDS             complement(812..973)
                     /locus_tag="PKZ53_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39313.1"
                     /translation="MENSRWSRGFGSYHRIIAQSHSAPGWALEYLSVNSPTLLRFFNP
                     APYRARAIG"
     gene            complement(1154..1276)
                     /locus_tag="PKZ53_02405"
     CDS             complement(1154..1276)
                     /locus_tag="PKZ53_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39314.1"
                     /translation="MKNDDFALPACTGLKLVQGAGVRLYVVDAPHLSGQLRLTG"
     gene            1399..3015
                     /locus_tag="PKZ53_02410"
     CDS             1399..3015
                     /locus_tag="PKZ53_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNJ39315.1"
                     /translation="MKRRRILLFAMVLMAMAPMVAIQPGQAQSQKSEATASFDSTSME
                     NDQVTEAELSPAQKAAITHAQTVSFDLLQARVPGIRSAREEFALRESFADQLQMAHVR
                     LDQTHQGVKVFQGELITHFQADGSLASITGDYFAGINISTTPRLTPNDAITIASEHFG
                     QLPSEPPTAELMILPKDGKYVLTYRTELVDIASETPAATVYFINAKSGKITWSYDNLE
                     TATATGSGRSLYSGTVPLSTNSITGKFELRDMVRGSHYTSDLKGRFLGTGTVFTDTDN
                     TWGSGVNADPSSAGVDAHYGAGKTFDYYKLVHGRNGIANNGQGGYSRVHYGRRYNNAF
                     WSDSCFCMTYGDGDGNTFSPLVSIDVAAHEMTHGVTSKTARLVYSGESGGLNEATSDI
                     FGTCTEFYAANSNDPGDYLIGEEITKTTRGWLRSMSDPRGDGASIDHYTQYTSGMDVH
                     YSSGLANHFFYLLSEGGTNRTSGMSVTGITRAKAEKIWYRALTVYMTSGTNYAAARTA
                     TLNAARDLFGGTTSAEYNAVAATWTACGVN"
     gene            3578..5053
                     /locus_tag="PKZ53_02415"
     CDS             3578..5053
                     /locus_tag="PKZ53_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39316.1"
                     /translation="MNYKKPPFPKDQDPNRQMTWYDLKELLFQAIKKQFRYVSPLIKN
                     QLKKLSPPQLESVLDILPQFSELQYLETHLAELSQKPEPDTDKDRFRQRPPSPERQIN
                     REFRPAPAFQRPEPSVEVARNPDTPGLKIEPGQERIIDTPSVLRADIEPEVSVSPVTV
                     PVQPPVQPPPVSHPMVTRQPLPAPPPVMPPSPITAPPVSATYAGVVRTTIPVLVGETQ
                     IDLVFEYDRFADRENHRGVRFLLSLDETQLLVARYLTQRERIQPIEPRVAEAIQSFFG
                     DAAQILNRAVQNLPSVSPEPVIPETAAPEPEPSVSPEPDQPGIEAAAHPAPVQLSLPV
                     EPVTETDEPDFEWSTPVSNAPRNPAPKRHRRTNQELAAAGISVEKRTRLTSEKIDLAS
                     LKFDPRLASILEIDQARQLNICPVSFLENSTDLLVLFVEPEGKVRVYTYFNRAANRAA
                     LAALVGITPENREQVKIRTFRVSQDFLDEFLNQAATMTGSE"
     gene            complement(5210..>5356)
                     /locus_tag="PKZ53_02420"
     CDS             complement(5210..>5356)
                     /locus_tag="PKZ53_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39317.1"
                     /translation="MQTQPALLGFALEQTKNDLPNIGYASVFPVATVLKIILVQVLFA
                     LLSH"
BASE COUNT         1367 a         1451 c         1302 g         1236 t
ORIGIN      
        1 acagaacagg aaggaaaaat gctgaaacac ctgttcccaa aagccacgct cctgatgaag
       61 gcagatgcga ctgaagaaac gatcaaaact gtccacaggc cagaggtgct ccatatagcc
      121 acacacggct atttttacgc tgatccagat caacccggtc cagatgcccc cgctgacacc
      181 cggtctgtga ctccgagatc agatgccgag gcaagtgaag aactccggat gcaaaaccca
      241 ttgacccgat cctacctttt ttttgcgggt gcaaatcgtg caggagatac tgaaaaggat
      301 gggacattga cggctctcga agcggctgga cttgacctgt ggggcactaa actggtggtc
      361 ctctcggcct gcgacacggg gttgggtgag attcgatttg gtgaaggaat ttatggactc
      421 cgacgggccc tggtgctggc cgggtctgaa acccaaatga tgagcctctg gccagtggca
      481 gacgtcgcca ccagcaagtt aatggtggcc tattaccagc ggttgaagaa agccgaaggt
      541 cgtagtcagg cgctacgaaa tgtgcagctc aactttctga aagatccaaa atatcgccac
      601 ccctattatt gggccagctt ttttacatct ggtgaatggg caaacctcgc cggagtgcgg
      661 taaggcagga aaaggtagct gagcaggcaa aacttccttc agtctggttt gcataatgtc
      721 ctccgttgcg gaaaggtcag ttttcgcccg gatgggcgcc ggaaaatagc cggtgggcag
      781 cctgctttga ggcgcaccca ccggaattca atcacccaat ggctcgcgcc cggtagggcg
      841 ctggattaaa aaacctcaaa agcgtcgggg agttgaccga cagatattcc agcgcccatc
      901 cgggcgcgga atgggactgg gcaatgatcc ggtggtagct cccaaagcct cgcgaccacc
      961 ggctattttc catcgccctc cgggcgggga ttgtcaagct caagtgacct ttccgcaacg
     1021 gagataatgt catcaacctt tgacagcact gaaccctact tgttaggatg gtctcctttt
     1081 cttcccctgc ccaagtcaga cattcgtttt gttcgcacgt ctgtccgtga tgcctggaca
     1141 aacacctatc agttcagccg gtcaaccgaa gctggcctga tagatgtggg gcatcaacca
     1201 catacaatct cacaccagca ccctgaacca gtttcagccc ggtgcaggca gggagagcaa
     1261 agtcatcatt cttcaaatca cttgccgacc tgggtcaccc caggccgccc gttccaccac
     1321 cggatacctg ctcacaggtg aaagaatcgt tttttggcct tcacaacaca ttgcaaagga
     1381 tagagagacc tcatacttat gaaaagacgc cgaattttac tgttcgccat ggttttgatg
     1441 gcaatggcgc ccatggtggc cattcagcca ggacaggccc agtcccaaaa atcagaagct
     1501 accgcatcct tcgactccac atccatggag aatgatcagg tgactgaggc tgagttgagc
     1561 ccggcccaaa aagccgccat tacccacgct cagactgtca gttttgacct gctccaggcc
     1621 cgcgtgcctg gtattcgtag tgctcgggag gaatttgccc tgcgtgaatc atttgccgat
     1681 cagttacaaa tggctcacgt ccgattggat caaacccacc agggcgtgaa ggtgttccag
     1741 ggcgaactga tcacccactt ccaggcggat gggtcactgg ccagcatcac gggtgattat
     1801 tttgccggga tcaatatttc aaccacaccc cgcctgactc caaacgacgc catcaccatt
     1861 gccagtgagc acttcggtca acttccaagc gaaccgccaa ctgccgaatt gatgattctg
     1921 cccaaagacg gaaagtatgt gctcacctat cgaactgagc tggttgatat tgccagcgaa
     1981 accccggcgg caacggtcta tttcatcaat gccaaatctg gaaaaataac ctggagctat
     2041 gacaaccttg agacggccac ggcgaccggc tctgggagaa gcctctattc cggcacagtg
     2101 ccgctcagca ccaattcgat tacgggtaaa tttgaactcc gcgatatggt ccggggcagc
     2161 cactacacat cagacctgaa aggtcggttt ttaggcaccg gcacggtatt taccgatact
     2221 gacaatacct ggggaagtgg tgtcaatgcc gacccatcca gcgctggcgt agatgcccat
     2281 tatggtgctg gcaagacctt tgattactat aaattagtgc atggtcgcaa tggaattgcc
     2341 aataacggcc agggcggata cagccgtgtt cactatggac gcagatacaa taatgccttc
     2401 tggagcgata gttgtttttg tatgacctat ggcgatggcg atggcaatac gttctcgcca
     2461 ctcgtttcaa ttgacgttgc agcacatgaa atgacccacg gcgtgaccag caaaacggcc
     2521 cgactggtgt actcaggtga atctggtgga ttaaatgaag ccacctcaga catttttgga
     2581 acctgcactg aattttatgc agctaattca aatgaccccg gtgactacct gattggcgaa
     2641 gaaatcacca aaaccacccg tggctggctg cgcagtatga gtgacccacg cggcgatggg
     2701 gcttcaattg accactacac ccagtatacc agtggaatgg atgtgcatta ctcgtcgggt
     2761 ctggccaacc actttttcta tctgctgtcg gaaggtggca ccaaccggac ctcagggatg
     2821 agtgtgacgg gaatcacccg ggccaaagcc gagaaaatct ggtatcgggc tctcaccgtc
     2881 tatatgacct ctgggaccaa ttatgcggcg gcgcgtacag cgacgctcaa tgcggcacgc
     2941 gatctgtttg gtggcaccac cagcgccgaa tataatgctg ttgcggcgac ctggacggcg
     3001 tgcggtgtaa attaatcgcc tccgcccaaa ttcaggtgga gagaaatgac ctgacatgac
     3061 aacacgggtt caggactgga caaatacttc aactcgaagt actttgtcag gcttgaaccc
     3121 gtttttatgt ccaaatacgc cttttctaac cctttgtaca acctgtcttg tgatgcccag
     3181 ggcttacgct tttaaatcac tgcaggtaac cagaattttc agactcaacg aggttccaac
     3241 gcacaatcca aaaatcaggt atgtcgggaa aaattgctca agtcgtcatt tgtttggaag
     3301 attacagctt acctggtttt tcttttctat cattttatcg tactttgggt acaatacgtt
     3361 tccttcaggg tattttcagc aaatcctggt tgtacgaagg atttcaggtt gtaaaaaacg
     3421 gtcggatgtg gccagttttt ctcagatcag agaaattgat cccagcctcc tggttttcaa
     3481 actggttttg gttctggcgc cacctgaaca ttctcacgaa gactgaaaat aaccggctca
     3541 tttcacaaac gacgttgatc cgttcaaact tacctttatg aattataaaa aaccaccctt
     3601 ccccaaagac caggatccaa accgacaaat gacctggtat gacctcaaag aactgctgtt
     3661 tcaggcaatc aaaaaacaat ttcgctatgt ttcgccactt atcaagaatc aactcaagaa
     3721 attgtcacca ccgcaacttg aatcggtgct tgatatcctg cctcagtttt ctgaactcca
     3781 gtatctggaa acccatctgg ctgagttgtc ccagaaacca gaaccagata ccgacaagga
     3841 ccggtttcgc cagcgaccgc cttcacccga aaggcagata aatcgcgaat tcagaccagc
     3901 ccctgccttt caaagaccgg agccatcggt tgaggttgct cgcaatcctg acacaccggg
     3961 actaaagatt gagccaggcc aggagcgaat cattgataca ccatctgttc tcagggctga
     4021 tattgaacca gaagtgagtg tttcaccggt gacggtccca gtccagcccc cagtccagcc
     4081 cccacccgtg tctcacccca tggtgacaag gcaaccattg ccagcgcccc caccggtgat
     4141 gccgccatcc cccataacag ccccacccgt atcggcaacg tatgccggag ttgtccgcac
     4201 cacaattcca gtcctggttg gggaaactca gatcgatctg gtgtttgagt atgaccggtt
     4261 tgcagatcgt gaaaatcatc gaggggtacg gtttttactc tcgctcgatg aaactcagtt
     4321 gctggttgcc cgatatctca cccaacgcga acgcattcaa ccaattgaac cccgagtggc
     4381 tgaggcaatc caatcttttt ttggtgatgc cgcacaaatt ctcaaccgtg ccgtacaaaa
     4441 cctcccttcg gtttcacctg aaccagttat cccggaaacg gcggcacctg agccagaacc
     4501 atctgtgtcg ccagaacctg accagccagg tatagaagcg gcagctcacc cagctccggt
     4561 tcaactatca cttccggttg agccagtcac cgagaccgat gaaccagact tcgagtggtc
     4621 aactccggtt tcaaatgccc ccaggaatcc agctcctaaa cgccaccgac gaaccaatca
     4681 agaactggcc gccgctggaa tttcagttga aaaacggacc cggttgactt cagaaaagat
     4741 tgacctggct tcgctcaaat ttgatcctcg cctggcatca attcttgaaa ttgatcaggc
     4801 ccggcagcta aatatttgcc cggtcagttt tcttgaaaat tcaaccgacc tcctggtttt
     4861 atttgttgaa cccgaaggaa aggttcgagt ttatacttac ttcaatcggg cggccaaccg
     4921 ggcggctctg gccgcactgg tgggaattac acctgagaac cgtgaacagg tgaaaattcg
     4981 aacctttcgc gtttcacaag attttctaga tgaattcctc aaccaggccg cgacgatgac
     5041 aggttctgaa taatacgagt ttgggattaa ggactcgcat tcagaagtca aggaactcca
     5101 atttttccag ttctttcgct tttgctcgtg tgagaatttc accccaaaac aggacacgga
     5161 ggcgagttgg tcgcctcctt tttattttga gaaccggcag gatgcggcat tagtgtgata
     5221 acagggcaaa cagaacctga accaggataa ttttcaggac cgtggctact gggaacaccg
     5281 atgcataccc aatattgggc aaatcgtttt tggtttgctc aagggcaaac ccaagcaaag
     5341 ccggttgggt ctgcat
//
LOCUS       DAOQDO010000094         5333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9818_length_5333_cov_2.821523, whole genome shotgun sequence.
ACCESSION   DAOQDO010000094 DAOQDO010000000
VERSION     DAOQDO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9818_length_5333_cov_2.821523"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="PKZ53_02425"
     CDS             complement(<1..1987)
                     /locus_tag="PKZ53_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39318.1"
                     /translation="MAVTVFGVAAIWLSSTPATSHGQAQEVPATAGHCISALDEFLSP
                     ETRFALPSSLVNAPLEDPEDADDEFELDDSSEAARQEATKVPKIKKVQPKRVAAAGPE
                     FRLRVNGENFKNGAQVLLNGTAVPTVFRNRRSIFAQVPTSLTATVGTISVVAKNPDGS
                     TTQPASFDVVNGLDGLEITALQPAIVLESTPDFVLKIAGTGFRDKIEARVAGIKTAQL
                     VRRRDPDGLVLLLVRSRDVAKAGSVPIQVQNKNGLSKVFNLLIAPPPPRIESTDPIRL
                     ELNAADTKVKIRGRNFTNKARVVASGTVIPSEFISDTRIDATFPAAFLKETRQLEVAV
                     EIPDEGISDFALLPVAATDPIIFGISPTRIVAGEPDFQVNISGINLTKQKKKTRVLVN
                     GESVKFEDTSGRELRIQIKKELVESPGSITIMVTVDDVTANTATLLVEPATTTSTMAG
                     DTPGFRDGLTTKAWFTRPSRAALGPDGKIYIADQLNHAIRQFDPSSGQVTTIAGDGIQ
                     GYVDTADIDTTDKNENNDRVRFNNPLGVAVDAAGIIYVSDFGNDVIRRIRRTDSQVTV
                     DTFAGENILSKNEDTGFKERVGRFGFINGNGNEARFSGPDGIILAPDGKFYVADAFNN
                     VIRVITPQGDDVQQPLVSAVFGLASPGIVDGEGIGIRF"
     gene            complement(2169..4400)
                     /locus_tag="PKZ53_02430"
     CDS             complement(2169..4400)
                     /locus_tag="PKZ53_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39319.1"
                     /translation="MDTPPNFQSSLTPSDHSVIQSDSDSPGWPNWLPGGRQIRPVLLA
                     TLLLGALILMGFGMARDYAWLKFDTEKYILEDRAIRILDWEHISSIFSTYYFINYNPL
                     HRLSYAIDYAIWGFDPGGFRTTNLLLHWTAGVFVFLCFYELTRRLTPAWLASICFVIH
                     PTRVESVVWLAQRKDVLSAAFGFATLWMYLRTWRPPVRSDGSETGLTQNQAGNKPSPD
                     VLPLFNPWWYAGCLVMYLCALASKAQWVPLCGVVVVIDLYRRQKMTRQRLIGYLPLVF
                     LSAVFAWYAIDSQLIVGKKGANNGFDLLDPLIQPVFGLGFYLWHTLWPVNLCPRYADF
                     IPEHSLFTLVGAIAVVMGGISVRQSWSGRREIFFGVAWFVMFLAPILNFLPGNLLVAD
                     RYLYVSLLGLVFPLGTWLSRQKTPLVIGFVGCVLVIQLGLTRQYVPHWKDDLTLWSYI
                     VSVSKENGFALTALAQAQIAHKNYPAARDSLNRALTYTPCFTPIYLTYVEYERELGGT
                     DALQLMSPALSTCPGLAELKAASGLYWTHRQEYRKAEACYLDSLRFRSTIEVLKQLAE
                     VYEKTNEPEKGIEVALRALQSYIFDEEVWLTLGKLLELQGNIPQAETAYLQCIQFDPS
                     QTESRYRLARIAFQKGDVAGAERWLGELRRVLNGKQLSDSAFTLWANIYREKKQYFLA
                     IACLLEAVQVKSTPENWLNLIHLQIEAGQDPQPAIKRAITLHPEIRAQIATDPKLQSV
                     NSE"
     gene            complement(4566..>5333)
                     /locus_tag="PKZ53_02435"
     CDS             complement(4566..>5333)
                     /locus_tag="PKZ53_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39320.1"
                     /translation="QELILTSERRVLIVDDEPLALQKLRRYLEHSGYPLQVIEAENGL
                     RAVELIREFQPEIIFLDIQMPGFSGFDVLRQFERRPFLVIFQTAFDQFAVRAFEEQAC
                     DYLLKPFTPARFQKAVTLAMERLVDEEKLQKLEVALAKEEGPLRRIAVKQGLRTRILE
                     DEEIHCFVSRDHCTCVYFGSQGEAILDLSLAYLITRLDPTRFRQFHRNNIVRIGAVCH
                     IVQTRSDELVIELCNGMKLPVSRSNRRIIRELLKEMC"
BASE COUNT         1436 a         1379 c         1298 g         1220 t
ORIGIN      
        1 tgaatcgaat tccaatacct tcgccatcca caatcccagg tgaagccagt ccaaaaacgg
       61 ctgacaccag tggctgctgg acatcgtcac cctgtggggt aatcacccga atgacgttgt
      121 taaaggcgtc agcgacataa aattttccat ctggtgccag gataattcca tcagggccgc
      181 tgaatcgggc ttcatttcca ttgccattga taaacccaaa ccgacccacc cgttctttaa
      241 atccagtgtc ttcgtttttg ctcaaaatgt tttcaccggc aaaggtatca accgttacct
      301 gggaatcagt acggcgaatt cggcggatca catcatttcc aaaatcagag acatagatga
      361 tacccgccgc atcaacggca acgccgaggg gattgttaaa ccgcacccgg tcattgtttt
      421 catttttgtc agtagtgtcg atgtccgccg tgtccacata cccttgaatg ccatctccgg
      481 caatcgtggt cacctggccg gaggatggat caaactggcg aatggcatga ttgagttgat
      541 cggcaatata aattttgcca tcaggtccaa gagcggcccg gctgggccgg gtaaaccagg
      601 ctttggttgt tagtccgtca cgaaatcctg gggtatcgcc agccatggtc gaagtggttg
      661 tggcgggttc aaccaggagg gttgccgtat tggccgtgac atcatccacg gtcaccataa
      721 tggtgattga acctggagat tccaccagtt cctttttgat ttgaatccgt aattcccgcc
      781 cgctggtatc ctcaaacttg acggattcgc cattgaccag gacccgggtt ttcttctttt
      841 gcttggtcag gttaattcca ctgatgttta cctggaaatc aggctcacca gcgacaatcc
      901 gggtcggtga aattccaaaa ataatcgggt cagtcgctgc caccggaagc aaggcaaaat
      961 ctgaaattcc ttcatcgggg atttcaaccg ccacttcaag ctgtctggtc tcttttaaga
     1021 atgcggcggg gaaggtggca tcaatcctcg tatcactgat gaattccgaa ggaatgaccg
     1081 ttccactggc gaccactcgc gctttgttgg tgaaatttcg gcctcggatt ttgactttgg
     1141 tatcagccgc attgagttcc aatcgaatcg ggtcggtgga ttcaatccgc ggcggaggtg
     1201 gggcaatcag caggttaaac acctttgaaa gaccattttt attctgaacc tgaattggaa
     1261 ccgacccggc tttggccaca tcccgcgacc gcacaagcaa cagaaccagc ccatccggat
     1321 cacgccgccg tacaagctgc gccgttttga ttccagccac gcgcgcttcg attttgtcgc
     1381 ggaagccggt tccagcaatc ttgaggacaa aatcaggggt gctttcaagc acaatggcgg
     1441 gttgcaaagc cgtaatttcc aacccatcca atccgttgac gacgtcaaat gaagcgggct
     1501 gagtcgttga accatccgga tttttggcca cgacggaaat agttccaaca gtggcagtta
     1561 ggctggtcgg aacttgggcg aaaatgctgc ggcggttgcg gaaaacggtt ggcaccgctg
     1621 ttccattcag caacacctga gcgccatttt taaaattctc accattgacc cgcagccgaa
     1681 attcagggcc agcggcggca acccgttttg gttgcacctt tttgattttc gggactttgg
     1741 ttgcttcctg gcgtgcggcc tcggacgagt catcaagttc aaactcatcg tcggcatcct
     1801 cgggatcttc cagtggcgcg ttcaccagcg aggatggcaa cgcaaaccga gtttccggtg
     1861 agagaaactc atccagggct gaaatacaat gaccagccgt tgcagggact tcctgcgcct
     1921 gcccatgtga agttgccgga gttgaagaca accagatggc agcgactcca aaaacagtca
     1981 cggccataag tatgtgaatc caccgttttg gtgaacgaaa cgcagaaaga tcagaaaatc
     2041 gacgagtatt cattgccatt aaaaccactg agaaaagttt agaggaacgt tgttgggaat
     2101 cctgcctgga aagccagcaa acatacgaga agccaacgca tagcgtcaag gtctggtcag
     2161 cgtttctgtt attcagagtt cactgactgg agtttcggat cagtcgctat ctgggctcgt
     2221 atctcgggat gaagagtaat tgcccgtttt atggcaggtt ggggatcctg tccagcttcg
     2281 atttgcaaat gaatcagatt caaccagttt tcgggagttg atttgacttg gacggcttca
     2341 agcagacagg cgatggctaa gaaatactgc tttttttcac gatagatatt tgcccacaac
     2401 gtgaaggctg aatctgaaag ctgtttccca tttaaaactc gtcgcaattc cccaagccat
     2461 cgttcagctc cagccacatc gcctttttga aaggcaatcc gcgccagacg ataccgggat
     2521 tcagtctggg atggatcaaa ctgaatgcat tgaagataag cagtctcagc ctgaggaata
     2581 ttcccttgta actcaagcag tttgcccaaa gtcaaccaaa cttcctcatc aaaaatgtac
     2641 gactgaaggg cacgcaaggc cacctcaatg cctttctcag gctcgtttgt cttttcatac
     2701 acctcagcaa gctgttttaa aacttcaatc gtggacctga atcgcaatga gtccagataa
     2761 caggcttcag ctttccggta ctcctgtcga tgggtccagt atagacccga ggcggccttc
     2821 aattcggcca gccctggaca cgtggagaga gctggggaca tcaattggag ggcatccgtg
     2881 ccgcctaact cacgttcata ttcgacatag gtcagataaa ttggggtaaa acagggagtg
     2941 taggtgaggg cccggtttaa ggaatcacga gctgccgggt aatttttatg ggcaatttgg
     3001 gcctgagcca acgctgtcaa ggcaaagcca ttctcttttg agaccgaaac aatatagctc
     3061 cataacgtca ggtcatcttt ccaatgaggt acatactgtc gcgttaaccc cagttggata
     3121 accagcacac atcccacaaa tccaatcacc agaggtgtct tttgccggct cagccaggta
     3181 ccaagtggaa agaccagccc gaggagtgaa acatagaggt aacgatctgc aaccaggaga
     3241 ttgcctggta aaaaatttaa gatcggtgct aaaaacatca caaaccaggc aacaccaaaa
     3301 aaaatttccc tccgacctga ccagctttgc cgaacactaa ttcctcccat cacaacagca
     3361 atcgccccca ccaatgtaaa caggctgtgc tccgggatga aatcagcata ccggggacag
     3421 agattgactg gccagagcgt atgccagaga taaaaaccaa gaccaaatac tggctgtatg
     3481 agtggatcca gcaggtcaaa gccattgttg gcaccttttt tcccaacaat aagttgtgaa
     3541 tcaatggcat accaggcaaa aacggctgag aggaaaacaa gcgggaggta cccaatcagc
     3601 cgttggcgcg tcattttctg ccgccgatat aaatcaatga ctacgacaac accgcacaat
     3661 ggcacccatt gggctttgga agccagagca cagagataca tcaccaggca tccggcatac
     3721 caccaggggt taaagagcgg gaggacatcg ggtgaaggtt tattccctgc ctgattttgg
     3781 gtcaacccag tttcagatcc atcggacctg acagggggcc gccaggtccg aaggtacatc
     3841 cacagggtcg caaatccaaa agcagccgat agaacatctt tgcgttgggc cagccacacc
     3901 acactttcaa ctcgcgtcgg atggatcaca aagcaaatcg aagccaacca ggctggcgta
     3961 agccgccggg tcaattcata aaaacaaaga aacacaaata ccccggcagt ccaatgaagc
     4021 agcaggttgg tggtacggaa cccccctgga tcaaatcccc agatcgcgta gtcaatcgca
     4081 taggacaacc ggtggagcgg attgtagtta ataaaatagt aagtggaaaa aattgaagaa
     4141 atgtgttccc aatccagaat ccggatggcc cgatcctcaa ggatgtactt ttcagtatca
     4201 aatttgagcc acgcataatc gcgagccatc ccaaatccca tcagaatcag tgctccaagc
     4261 agcagcgtgg ccagcaagac aggtcggatt tgtcgccctc ccggcaacca gtttggccag
     4321 ccaggggagt cagaatccga ctgtatgaca gaatgatccg atggtgtcag cgatgactgg
     4381 aaatttggtg gggtgtccat atcgttacag ataggaaaaa attgacgtaa cccaacgtgg
     4441 aatctggggg taaggtcgtg tgatcttggg aaacaacaat ccctaaagga cctcaagtga
     4501 gcacaagagg tcaggtgccc aggcaatcac tccgaaaacg tgcctgtgca gaacccccct
     4561 cagtactaac acatttcctt gagtaattca cgaatgattc gtcggttgct gcgggaaaca
     4621 ggtaatttca tcccattgca gagttcgatc accagttcgt cactgcgcgt ctggacgata
     4681 tgacacacag ccccaattcg gacaatattg ttgcgatgaa attgccgaaa ccgggtcggg
     4741 tccaatcggg tgatcagata agccaatgac aaatcaagaa ttgcttcgcc ctggctccca
     4801 aaatacacac aggtacagtg gtcacgactg acaaaacagt ggatttcctc atcttctaaa
     4861 attctggtac gcaggccctg tttgaccgca atccgtcgta atggcccttc ctctttcgcc
     4921 agcgcgacct ctagtttctg gagtttttct tcatcgacaa gccgttccat ggccagcgta
     4981 accgcttttt gaaacctcgc cggcgtaaat ggcttgagca agtaatcaca ggcctgttct
     5041 tcaaaagccc gcacggcaaa ctgatcaaat gccgtttgaa aaataaccag aaaggggcgg
     5101 cgctcaaatt gccgcaacac atcaaaccct gaaaaaccag gcatttgaat atccaagaaa
     5161 ataatctcgg gttgaaactc gcgaatcaat tccactgccc gcagaccatt ttcggcttca
     5221 atcacttgca gtggatagcc actgtgctcc agataccggc gaagtttctg aagcgccaga
     5281 ggctcgtcat ccacaattaa aacacgccgt tcgctagtca agatgagctc ctg
//
LOCUS       DAOQDO010000095         5329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9826_length_5329_cov_2.894008, whole genome shotgun sequence.
ACCESSION   DAOQDO010000095 DAOQDO010000000
VERSION     DAOQDO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9826_length_5329_cov_2.894008"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKZ53_02440"
     CDS             complement(<1..1190)
                     /locus_tag="PKZ53_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39321.1"
                     /translation="MRNDFWCAIRSLKQQAGFWLIAVVTFALGIGANVSIFSVFNGVL
                     LKPLPYPAPDRIVHCFTSHLERKGEFSNSAHDFADWRQLNQCFDSMASYRSIDLNYSG
                     ADQPIRFQGSLVSPGFTRVLGVSASLGRTFTQEEEQSGKHQVVLLSHKTWKGAFGADP
                     NIIGRTLTLNQEPYQVIGIMPEGFGFPTPEVEVWTPLTLDLSPDTRGNHYLRSIARLK
                     PGVSLPQAQSEMDAISRDLQQRFPETNALSGVKLVSMYERLTRDIRPALLVLLAAAIA
                     VLLIACANIANLQLARMTARSREIAVRLAVGASRWQLLRLFLVESWLLTLPGGVLGIV
                     LAVLGTPYILKIDPTNIPRVAEIGLDWRVLAFAIGLTVVTGLGFGITPIFQAFEDNVG
                     EKLK"
     gene            1424..3268
                     /locus_tag="PKZ53_02445"
     CDS             1424..3268
                     /locus_tag="PKZ53_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019025983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNJ39322.1"
                     /translation="MTFSLSASTFAQIGPDAHRDIHSFSAPDQIRVKHVDLDLEVRFE
                     QKMLKGTATLELERFSKDAATPLRLDTRDLTIFKAEASADGKTFSETKFELGAADAIL
                     GAPLTVQLPAQATHVRITYETSPKASGVQWLDPSQTAGKKSPYMFTQSQAIHARSWIP
                     LQDSPQVRVSYNARIRTPKNLLAVMSADNDPKTRRDGDYSFKMAQPIPSYLIALAVGD
                     IKFQSLGKRSGVYTEAAMLKKAAKELNDTEKMIEITEKLYGPYRWGRYDLLILPPSFP
                     FGGMENPKLTFATPTILAGDKSLVSLIAHELAHSWSGNLVTNATWRDFWLNEGFTTYL
                     ERRIVEAVYGKDQERMEAVLGRQDLQENLDDFEERDEILYVDLKDRDPDDGFTKVPYE
                     KGALFLRHLEDAFGRERFDVFVRNYFDHFAFKSITTGDFLDYLNKNLMSQDAKAAAQI
                     PLEEWIFKAGVPANAPKPMSDAFSNVENQANRWLSGEIPASAMNTQKWISHEWLHFLR
                     FITTQPVAPAKLAELDKAFGLTRSGNSEVAHQWLLLAIRNRYEAAYPKLEEYLVTIGR
                     RKLIKPLYEELVKTPEGRDRAMSIYRKARPGYHPITIATVDGIVKWQG"
     gene            3572..>5329
                     /locus_tag="PKZ53_02450"
     CDS             3572..>5329
                     /locus_tag="PKZ53_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39323.1"
                     /translation="MSGNHLVQNQPPKHRQSAASSQRPGPKPKGLAGPKTTTAAKSAP
                     HQENTRLSKTIQGTATISANRQKAPTAQSLHELTARQLERRQRSEKTEREKRPPTLAD
                     PGARLRTGPPTIPATNLGTTARQSSSIQLLTNTSLGEVSSDKSLVSEPTAAEGGNNVF
                     YTANWFAAVSTDGGGSFRYVDPYNFFPSAADGFCCDQDTVFAPTQNLMVWLLQYVNNA
                     SGNVVRIAVTSNPAGDWMYYDFEPELIEGISDSDWFDYPHLSISNNFLYFTINIYTRS
                     DDYRASAIFRAPLSELAAGGDLSIGYYATGEDFFSSPAQGATTTMYWAGHRTNSSLRI
                     FSWPESSPNVAINDVTHAAYPFRDNTCVDGDGANPCQRSDDRVLSAWVSGGKAGFLWN
                     AARGVGGLGVLPYPYVHYVTINESSKSLVEEGAIWNPSFAYFYPSLAPNAAGKLGGVI
                     GWAGGRLFPSFGALVSKSASGTELSNLDILAVGVGNRGPNSDVWGDYLRVRQSSLDPS
                     GWVASGYNLQGSGSGFGAVPRYIRFVATEGGNEGDTIAPGVSVLAPVGGAKIKTFSFQ
                     VSWTSQDNVGVTSHDILLSN"
BASE COUNT         1339 a         1436 c         1343 g         1211 t
ORIGIN      
        1 cttttcaatt tttcgccgac gttgtcttca aatgcctgaa agattggcgt gatcccaaat
       61 cccagcccgg tcaccacggt cagaccaatg gcaaaggcca gcacgcgcca atcaagtcca
      121 atttcagcca ctcgcggaat attggttgga tcaatcttga ggatatatgg ggttccgagc
      181 accgccagca cgatgccaag caccccgcca gggagcgtca acaaccagct ttcaaccaga
      241 aacagccgca aaagctgcca ccggctggcc cccaccgcca ggcgaaccgc aatttcacgg
      301 ctacgagcgg tcattcgagc cagttgtaaa ttggcgatat tggcgcaggc aatcaaaaga
      361 accgcgatgg cagccgccaa aaggaccagc agtgccggtc ggatatcacg agttaatcgc
      421 tcatacatcg aaaccagttt caccccgctc agggcgttgg tttcaggaaa ccgctgctgc
      481 agatccctgg aaatcgcgtc catttccgac tgagcctgcg gcaggctcac cccaggtttc
      541 agtcgggcaa tcgagcgcag atagtgattt ccacgagtat cgggtgacag gtcgagcgtc
      601 agtggcgtcc acacctcaac ctcaggtgtt ggaaaaccaa acccttcggg cataatgcca
      661 atcacctgat agggttcctg attgagggtc agggttcggc caatgatatt tggatcggca
      721 ccaaaagccc ctttccaggt tttgtgactc aacaagacga cctgatgctt tcccgactgt
      781 tcttcttcct gggtaaaggt tcgccccagc gacgccgaga caccaagaac gcgggtaaac
      841 cccggtgaga cgagcgatcc ctggaaccgg atcggctgat cggcgccaga ataattcaga
      901 tcaatagagc gataagacgc catgctgtca aagcactgat tgagctgtcg ccaatcggca
      961 aaatcgtggg ctgagttgct aaactctcct tttcgctcaa ggtgcgaagt aaaacagtgg
     1021 acgatccggt cgggtgccgg atagggtaat ggcttgagta acaccccatt aaacacactg
     1081 aaaattgaaa cgttcgcacc gattccaagc gcgaatgtca ccacggcaat caaccaaaaa
     1141 ccagcctgct gtttgagcga acgaatcgca caccaaaaat cattgcgcaa cccatgcata
     1201 agaatccctc acgaaaacag aaaatgttga gtactggaga tgcctggttg gagagaaaca
     1261 cagaatacgg cggccagcag tcaaattcca gttgtcgtgc atctattgac ttgctaaaaa
     1321 gcttctttct ttcaacaccg gcgcacactc catttcaccc ccaatccctt tcggagtttt
     1381 tctgatgaaa tttgctattc gtcttgctct ccttgtggtg cttatgacgt tctcactctc
     1441 tgcttcaacc tttgcccaaa ttggccctga tgcgcatcgt gacatccact ccttttccgc
     1501 tccagaccag attcgggtca aacacgtgga cctggatctg gaagttcgct ttgagcaaaa
     1561 aatgctcaaa gggacagcga cgctggaact cgaacgcttc tcaaaagatg ccgctacgcc
     1621 gttgcggctc gatacgcggg atctcaccat cttcaaagct gaggcgtcag ccgatggaaa
     1681 aacatttagc gaaaccaaat ttgaacttgg tgccgcggat gcaattcttg gggccccgct
     1741 gacggtgcaa cttccggccc aggccaccca cgttcgcatc acctatgaaa ccagccccaa
     1801 agcttcgggg gttcagtggc tcgacccatc gcaaacggct gggaaaaaat cgccctacat
     1861 gtttacccag tcacaggcca ttcacgcccg gagctggatt ccattgcagg acagcccgca
     1921 agtccgtgtg agctacaatg cgcgaatccg cacccccaaa aatctgctgg cggtgatgag
     1981 tgccgacaac gacccgaaga ccagacggga tggcgattac tcgttcaaaa tggcacagcc
     2041 cattccctcg tatttgattg cgctggcagt cggagacatc aagtttcagt cacttggcaa
     2101 gcggagcggt gtgtataccg aagccgccat gctgaaaaaa gccgcgaagg aattaaacga
     2161 caccgaaaaa atgatcgaga ttaccgaaaa gctctatggt ccctatcggt ggggacgcta
     2221 tgacctgctc attttgccgc cgagcttccc gtttggcggg atggaaaatc caaaactgac
     2281 ctttgccacg ccaaccattc tggccggaga caaaagcctg gtttcactga ttgctcacga
     2341 actggcccac tcctggtcag gcaatctggt gaccaatgcc acgtggcgtg atttctggct
     2401 caatgaaggg tttaccacct atctcgaacg gcgaatcgtt gaagccgtct atggcaaaga
     2461 ccaggaacgg atggaagcgg tgctgggccg ccaggatttg caggaaaatc tcgatgattt
     2521 tgaagagcgg gacgaaattc tctatgttga cttgaaagat cgtgacccgg atgacgggtt
     2581 taccaaagtt ccctatgaaa aaggggcact cttccttcgc catctggaag atgcctttgg
     2641 tcgcgaacgc tttgacgtgt ttgtccgcaa ttactttgat cactttgcct tcaagagcat
     2701 taccacgggc gattttctgg actatctcaa taaaaacctg atgagccagg atgccaaagc
     2761 cgccgctcag attccgcttg aagaatggat tttcaaagca ggtgttccag ccaacgcgcc
     2821 caaaccaatg tctgatgcct tcagcaacgt ggaaaaccag gccaaccgct ggctctcggg
     2881 tgagattcct gccagtgcaa tgaataccca aaaatggatt tcgcacgaat ggcttcactt
     2941 cctgcggttt atcacgaccc aaccagtggc acctgccaaa ctggctgaac ttgacaaagc
     3001 atttggactc acccgttccg ggaactcaga agttgctcat cagtggttac tgcttgccat
     3061 tcgcaatcgc tatgaagcgg cctatcccaa actggaagaa tatctggtca ccattggccg
     3121 ccggaagttg atcaaaccgc tctatgaaga actggtcaag actccggaag gtcgtgaccg
     3181 ggccatgtct atctatcgca aagcacgccc aggttatcac cccatcacca ttgccacggt
     3241 tgacggcatc gtgaagtggc aaggatagca atggaatgaa gaatgaagaa tgaagaatga
     3301 agaatgaaga atgaagaaaa ttcttggtgc ttggtttttg gttcttggtt cttggttctt
     3361 ggttcttggt tcttagaaga cattgatttc caagaaccag gcaccagaga cattcttcat
     3421 tctcaattct tcattcaaag aaggtagcct tatgttcaca actcatcgaa agttccgtca
     3481 gtcacgatca ggattcagag tttggcgccg agcatttctt gttttggggg gagtaattgc
     3541 cctgggaagt tttttgagac tgtctcaaac tgtgtctgga aatcatctag tacaaaacca
     3601 acctccgaaa caccgccaat cagcagcctc cagtcagcga ccagggccaa aaccaaaagg
     3661 actcgctggc cccaaaacga ccacggcggc caaatcagcg cctcaccagg aaaatacccg
     3721 actttcaaag acgattcagg gcacagccac gatttcggcc aatcgtcaga aagcccccac
     3781 ggcccaatca ctgcacgaat tgaccgcccg gcaacttgag cgccgacaac ggtctgaaaa
     3841 aacagaacgg gaaaaacgtc cgcccactct tgctgatcca ggggcgcgat tgcggactgg
     3901 accaccgacc attccagcca caaacctggg aacaaccgcc cgtcaatcca gcagcattca
     3961 acttttaacc aacacctcac tgggcgaagt atcttctgac aaaagcctgg tcagcgaacc
     4021 gacagccgcc gaaggtggaa acaatgtgtt ttacacggcc aactggtttg cggcggtttc
     4081 aactgatggc ggcgggtcat ttcggtatgt ggatccgtac aatttttttc cttccgccgc
     4141 cgatgggttt tgttgtgacc aggacaccgt gtttgcccca actcagaacc tgatggtgtg
     4201 gttgttgcaa tacgtcaata atgcttctgg caatgtggtc cgaatcgccg tcaccagtaa
     4261 cccggcgggt gactggatgt actatgattt tgagccggaa ttgatcgaag gaatttccga
     4321 ttcagactgg tttgattacc cacatctctc aatcagtaac aactttttgt atttcacaat
     4381 taatatttac acacgttcag acgactatcg ggccagtgcc attttccgcg caccactcag
     4441 cgaactggcc gcgggcggag atctttcaat tgggtattat gccaccgggg aagatttttt
     4501 tagctcacca gcccagggtg ccaccacgac tatgtattgg gccgggcatc ggaccaacag
     4561 ttccctccgc atttttagct ggccggaatc aagccccaac gttgccatca atgatgtgac
     4621 acacgccgcc tatccatttc gggacaatac ctgtgttgat ggtgatgggg ccaacccgtg
     4681 ccagcgatca gatgaccggg tgttatcagc ctgggtttcc ggtgggaagg ctggcttttt
     4741 gtggaatgcg gcacggggtg tgggtggact gggggtactt ccttacccat atgttcacta
     4801 tgtaacaatc aatgaatcct caaaatcact ggttgaagaa ggggcgattt ggaatcccag
     4861 ctttgcttat ttctaccctt cgctggcacc aaatgccgcc gggaaactgg gcggggtcat
     4921 cggctgggct ggaggacggc tgtttccctc gtttggggca ctggtctcaa aatcagcttc
     4981 gggcacagaa ctatcaaacc tggacattct ggccgtcggt gtcgggaatc gtgggcctaa
     5041 cagtgatgtc tggggagatt acctgcgcgt tcgtcagagc agtcttgatc catctggttg
     5101 ggtggcaagt ggatacaatt tacaaggcag tgggagcggt tttggcgccg tgccacgcta
     5161 cattcggttt gtcgcaactg aaggtgggaa tgaaggcgat accatcgcgc caggggtttc
     5221 ggtgcttgct ccagttggcg gcgccaaaat caagacgttt tcatttcagg tgagctggac
     5281 ctctcaggat aacgtcggag ttacctcaca cgacattctg ctttcaaac
//
LOCUS       DAOQDO010000096         5321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9848_length_5321_cov_3.796810, whole genome shotgun sequence.
ACCESSION   DAOQDO010000096 DAOQDO010000000
VERSION     DAOQDO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9848_length_5321_cov_3.796810"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKZ53_02455"
     CDS             <1..379
                     /locus_tag="PKZ53_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNJ39324.1"
                     /translation="TSPLFSQSILVDKMRGRLTCDDVVKAAIANDELSRMVLIETAGY
                     LGTAVANAVNLLNVEKVILGGPVMAAGEFLLDFIRDEVSKQAFTPLFASCQIHMGQLG
                     DDAGEIGAALMARDFTKGQAATK"
     gene            complement(369..2297)
                     /locus_tag="PKZ53_02460"
     CDS             complement(369..2297)
                     /locus_tag="PKZ53_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ39325.1"
                     /translation="MGILFFGASLLFCAQVTSAQSPTPVLTLPSTWKMSPGDNPDWAK
                     PDFDDSGWETVNISKAWIEEEGHDYRHTGWYRLTVHLNLTSFESACGLAVSPVTFSGY
                     EVFVNGKRIGQIGKFSPLGQTFVPVTTIFPLSSELVDASGRVVIALRTRWDQPFAKAF
                     YRWRTFPASTCRIGNFAYLSLEAQTGIQQDLLQDVPRLFVASLIALVGLYHLLLFAQR
                     RQLKEYLWFALITFSISLVAISISLWPARHFAVENLMAGTRVGSFFAIIFGFEFADDL
                     FGTGNTKWVRSIQALLVLCVVLVVVYPPVVMTQFTRVSLSFPLIWGIWFSTVVFRKAW
                     LGNTDARIISVGIVGVIVSQIFMLASVEGYLPAYPPGPVGFLVLVVSMLISLSTRFSR
                     INAELDALNRDLEAKVEQRTLELADANQQLAAANAILHDENRLVRERELEARTHAQQA
                     QLQMLRYQLNPHFLFNVLNSIGVLAFIDPKRTFQMVTGLSEFLRYSLQTTDITLAPLK
                     DEIKAIRHYLELQQVRFEERLEIQFEISPDSENIRLPAFLIQPLVENAIKYGMQTSPK
                     RLLIRLMARQHRNRLELRIANTGQWVSEVDRPLFEEGTGIGLSNVQTRLAKSQFRRTD
                     FRIFQSNGWVWVRIRVTS"
     gene            complement(2387..3136)
                     /locus_tag="PKZ53_02465"
     CDS             complement(2387..3136)
                     /locus_tag="PKZ53_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNJ39326.1"
                     /translation="MPRPITAVIVDDERLARMALRMLLADHPQVQIVGEAASVPEAVE
                     VIQKTTPEVVFLDIQMPDGIGFDLLNRVEGTFKIVFVTAYDSFAIRAFEVNALDYLLK
                     PVTPERLAQTLARLDSLPPDSGSEPGAGDSPDSSKFTYDDAVFLTSGQRQQFVKINHI
                     QVIQAERDYSHVITADGRRAFVRKSMTEWEERLPSRHFLRVHRSIIVNLDYVDRVERS
                     PNSSYCVFLRGAEKPFPMGRRFLSVLREQIR"
     gene            3457..4194
                     /locus_tag="PKZ53_02470"
     CDS             3457..4194
                     /locus_tag="PKZ53_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39327.1"
                     /translation="MYKSTLLIFVATDQEFQALSSMLGQWRPEKATPRRGTGWLAGYQ
                     IDLVQTGMGPENAAHRSRTLLKHHPARTVLMCGIAGALSPQCQVGDAVLYTHCQSSAR
                     PDIQIKTTSSTTESIVSSLETGSIRTFQGTGVTLPRVVCRASEKVQIGSEYRALAIDM
                     ESYCVVENALQANRHVAVLRIISDDTRHDLPDFNQAINNQGHINHLKMALVLGRTPIR
                     TLAFARNLQFSMAQLKKAFHLALSAKL"
     gene            4340..>5321
                     /locus_tag="PKZ53_02475"
     CDS             4340..>5321
                     /locus_tag="PKZ53_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNJ39328.1"
                     /translation="MLKALLKNRTLTSRVIALVGLISILSGIAAPFGNQVLADESPQI
                     DKGGVKVQVIDLKDLPVPNATITVKLIKTQAGVEVANQTPYQKITNDKGEAIIPELAP
                     GIYYLTIEAEGYQPLNEPEVEVMAGAISTLSIQMPPKITSEDTVEVRAESDKLIEQAA
                     APQAQVTTQIIRTAPLLSRTFDAALPIVPNVIRDPNGKISIKGGREDQSALLVNNADS
                     TDPATGNFALSIPLESIEQVKVFTNPYLPEYGRFTGGVTKVETKRGGEKWKAEVYDFF
                     PEPRFRGGKLFGLANFSPRLHFEGPILKDRLYFSQGLEYDVDKKPVRGLAS"
BASE COUNT         1445 a         1348 c         1323 g         1205 t
ORIGIN      
        1 tacgtcacct ttattctcac agtccattct ggttgacaaa atgcgtggcc ggttgacctg
       61 tgatgatgtg gtcaaagcgg cgattgccaa cgacgaactt tcccggatgg tactgattga
      121 aaccgccgga tacctcggca ctgctgtggc caacgccgtc aatttgttga atgtggagaa
      181 agtcatcctg ggtggtccag taatggcggc aggtgagttt ttacttgatt ttattcggga
      241 tgaagtttcc aaacaggcat ttacgccatt gtttgcgtcc tgccagattc atatgggtca
      301 acttggcgat gatgccggcg agatcggggc cgcgctgatg gcacgagact tcaccaaagg
      361 gcaggcggct acgaagtgac ccgaatccgg acccacaccc agccattcga ttgaaaaatt
      421 cgaaaatccg tccgccggaa ctgacttttg gccaatcggg tctggacatt gctcaagcca
      481 attcccgttc cttcctcaaa gagtggccgg tccacttctg aaacccactg accagtattg
      541 gcaattctta attccagccg gttccgatgc tgtcgggcca taaggcgaat caacaaccgt
      601 ttcgggctgg tttgcatacc gtacttaatc gcattttcca ccagcggctg gatcagaaat
      661 gctggtaatc gaatattttc cgaatctggc gaaatctcaa actggatttc aagccgctct
      721 tcaaacctga cctgctgcaa ttccagataa tgccgaatcg ctttgatttc atccttgagc
      781 ggcgccagcg tgatatcagt ggtttgaagt gaataccgga ggaattctga caatccggtg
      841 accatttgaa aggttcgttt cggatcaata aacgccagaa ccccaattga attcaacaca
      901 ttaaacagaa aatgagggtt gagctggtac cgcagcatct ggagttgcgc ctgctgggca
      961 tgagttcggg cttcgagttc acgttcacga accagtcggt tttcatcgtg caaaatcgcg
     1021 ttagctgcag ccagttgctg attggcgtca gccagttcga gtgtccgttg ttcaactttg
     1081 gcttccagat ctcgattaag ggcatcaagt tccgcattga tccgactaaa gcgagtcgaa
     1141 agtgagatca acatgctgac caccagcacc agaaacccaa cggggccagg tggataggcg
     1201 ggtaagtacc cttcaaccga agccagcata aagatttggg aaacaatgac tccgacaata
     1261 ccgacactga taattcgggc atcggtgttt ccaagccagg ctttccggaa cactacggtt
     1321 gaaaaccaga ttccccaaat cagcgggaag ctcaatgaca ccctggtaaa ttgggtcatg
     1381 acaaccggag gataaaccac caccagcacc acacacagca ccagcaatgc ctggatactc
     1441 cgaacccatt tcgtgttccc cgttccaaac agatcgtcag caaattcaaa accgaaaata
     1501 atggcgaaga atgacccgac tcgggttccg gccatcaagt tctcaacggc aaagtgtctg
     1561 gccggccaga gcgaaatgct gatcgcaacc agggaaattg aaaaggtaat aagtgcaaac
     1621 cagagatatt ctttgagttg ccgacgttgg gcaaacaaaa gcagatgata cagaccgacc
     1681 agggcaatga gacttgccac aaagagccgt ggaacatcct gaagcagatc ctgttgaatc
     1741 ccggtttgtg cttccaacga cagataggcg aaatttccaa tccgacaggt tgaggctgga
     1801 aaagttcgcc accggtaaaa ggctttggca aagggttgat cccatcgagt ccgcagggca
     1861 atcacgaccc ttccagaagc gtcaaccagt tctgacgaga gcggaaaaat ggtggtaaca
     1921 ggcacaaagg tctgacccaa aggagaaaac ttgccaattt ggccaattct tttcccattt
     1981 acaaacactt catacccact aaaagtcacg ggactcactg ccagcccaca agccgactca
     2041 aaagaagtca gattgaggtg aaccgtaagg cgataccagc cagtgtggcg atagtcgtgg
     2101 ccttcttctt caatccaggc ttttgaaata ttgacagttt cccacccgga atcgtcaaag
     2161 tcaggttttg cccagtcggg gttatcgcct ggactcattt tccaggttga aggaagggtc
     2221 aagaccggag ttggggattg ggcggaagtt acctgcgcac aaaacagcag cgaggcccca
     2281 aaaaacagta tccccatcag acaccatcga atgagggaac cattgtaatt taaggagtta
     2341 acgtagtttc ccatcagagc gcagtgtcaa gaaaaaagaa aaaagcttat cgaatttgtt
     2401 cccgcagaac ggaaagaaac cgtcggccca ttgggaacgg tttttcagct ccacgcaaaa
     2461 agacgcagta tgaagaattg ggagatcgct ccacccggtc tacataatca agattgacaa
     2521 tgattgagcg atgaacccgc aaaaaatggc ggcttgggag gcgttcttcc cattcggtca
     2581 tggatttgcg gacaaaggcc cgccgtccat cagccgtgat cacgtgtgaa taatcgcgct
     2641 cggcctggat gacctggatg tggttgattt tgacaaattg ctgtcgctgg cctgaggtga
     2701 gaaagacagc gtcatcatag gtaaacttgc tggaatctgg tgagtcacca gcacctggtt
     2761 cggaacctga atccggcggc agggaatcaa gccgcgccag ggtttgcgcc aggcgttcgg
     2821 gtgtcactgg tttgagcagg taatcaaggg cattgacttc aaaggcgcga atggcaaatg
     2881 aatcgtaggc agtcacaaaa acaattttga acgttccttc aacccgattc aggaggtcaa
     2941 acccaattcc atctggcatt tgaatatcga gaaacacaac ctcgggcgtt gttttctgaa
     3001 taacttcaac ggcttcgggc acagaggcgg cttcgcccac aatctgaacc tgtgggtgat
     3061 cagccagaag catccggagc gccattcgcg ccagacgttc atcatccaca atgacggctg
     3121 tgattggtcg gggcataaaa atccaagggt tccgggttcc gggttccggg ttccggaaaa
     3181 cctgaactat aaatgagcca aattgcgggg gcgggcgtga aactgagaca atgtattatg
     3241 aatgatatga tgcaatttcg gaaacccgga acccagatgg aatgacaggg tgacaagatg
     3301 acagagtaaa acttatccga aaacgtggaa ccagagttcc aggccaccaa caatctgccc
     3361 caattcaaac aggcttttgc ggcaaaaagc acgagcacta cagattcgaa aacccggaac
     3421 ccggaacccg gaacccggaa cccggaaccc ctaaccatgt acaaatccac tcttctcatc
     3481 tttgtcgcca ccgatcagga atttcaggca ctctcatcaa tgcttgggca atggcgcccg
     3541 gaaaaagcta cgccacgacg gggaaccggc tggctggctg gctatcagat tgacctggtc
     3601 cagactggaa tggggccaga aaatgccgcc catcggtcac gaaccctttt aaagcaccat
     3661 ccggctcgaa ctgtcttaat gtgtgggatt gctggggcgc tttcacccca atgtcaggtt
     3721 ggtgatgcgg tactctatac ccactgtcag tcttccgctc ggccagatat tcaaattaaa
     3781 acaacttctt ctacaactga atcaatagtt tccagccttg agactggttc aattcgaacc
     3841 tttcagggaa cgggtgtaac gttacccaga gtggtgtgtc gggcaagtga gaaagtacag
     3901 atcggaagtg aatatcgagc gctggcaatt gatatggaga gttattgtgt ggtggaaaat
     3961 gcgctccagg caaatcggca cgtggcggta ctgcgtatca tcagcgatga tacccggcat
     4021 gatttacctg atttcaatca ggccataaac aaccaggggc acatcaatca cctcaaaatg
     4081 gcgttggttt taggccggac accaatccgg acactggctt tcgccagaaa cctccagttt
     4141 tcaatggctc aactcaagaa ggcatttcac ctcgcactga gcgcgaaact ttagtaaaat
     4201 gccgcgtttc tttttcggta gttgtttcgg aagaagtcaa gcggggaccg aatttgattc
     4261 agtacaccag ggttcaccag attgactttt ttcaggctcg caccttttac tcactatgaa
     4321 aacaaacttt tctgttcttt tgttgaaagc tcttttgaaa aaccgcacgc tgacctctcg
     4381 ggtcattgcc ctggttggac tcatttcgat cctgtcaggg attgccgctc cgtttgggaa
     4441 ccaggtcctg gccgatgaat cacctcaaat cgacaagggc ggcgtcaaag tccaggttat
     4501 tgacctcaaa gacctcccgg ttccaaatgc gaccatcact gtcaaactca ttaaaacaca
     4561 agccggtgtc gaagttgcca accaaactcc ataccaaaaa attaccaacg acaaaggcga
     4621 agcgattatt ccagagcttg cgcccggcat ctattacctc acgattgaag ctgagggcta
     4681 tcaaccgtta aacgagcctg aagttgaggt gatggctggg gccatttcga cactttcaat
     4741 ccagatgccg ccgaaaatca cttcggaaga cacagttgaa gttcgcgccg aatctgacaa
     4801 gttgattgaa caggcggcgg caccacaggc acaagtcacc acacaaatca ttcgtaccgc
     4861 gccacttctg agccggacgt ttgacgctgc gctgccgatt gtccccaatg ttatccgcga
     4921 cccgaacggg aaaatcagca ttaaaggtgg ccgtgaagac cagagcgctc tgcttgtcaa
     4981 caatgctgac tcaactgatc cagccactgg aaatttcgcc ctgagtattc cgcttgaatc
     5041 catcgaacag gtcaaagtct ttaccaaccc gtatctccct gaatatggac ggtttactgg
     5101 tggtgtgacc aaagtggaaa ccaagcgtgg cggtgaaaaa tggaaagctg aggtctatga
     5161 cttctttcct gaaccacgat ttcgcggcgg taaactcttt ggtttggcga acttctcgcc
     5221 tcgattacat ttcgaaggcc cgattctgaa agacagattg tatttctcgc agggcctgga
     5281 atatgacgta gacaaaaaac cggttcgtgg attggcctcg c
//
LOCUS       DAOQDO010000097         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9862_length_5315_cov_3.470152, whole genome shotgun sequence.
ACCESSION   DAOQDO010000097 DAOQDO010000000
VERSION     DAOQDO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9862_length_5315_cov_3.470152"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..802)
                     /locus_tag="PKZ53_02480"
     CDS             complement(185..802)
                     /locus_tag="PKZ53_02480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNJ39329.1"
                     /translation="MDISPVILEGQHVRLEPLSPTHEESLIAAASDGELWNSTVTIVP
                     DQTNMAAYIAEALHGQAQGIQLPFVIVQKSSGQIVGSTRFYNIDQNNRGVEIGYTWLA
                     ASAQRTSVNTETKLLLLTHAFEVWKCIRVAFITDVLNHQSRTAIVRLGAREEGILRNH
                     MIMPNGRVRDSVSLSIIESEWPEVKARLEAKRSLTVDATQDGFSE"
     gene            complement(827..2791)
                     /locus_tag="PKZ53_02485"
     CDS             complement(827..2791)
                     /locus_tag="PKZ53_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ39330.1"
                     /translation="MNKQIKHFYEFGLFSIDIGNRLLLRDGEPVALQPKTFDTLLLLV
                     ENSGRVLEKNEILDKVWPDTVVEESNLTQNIYILRKVLTDPSSQQKYIETLPRRGYRF
                     IAGVEEVWNEEGADLPVEEHPQATIVVEENQDEVRQERETQPEDTEAALPVAAPELGS
                     PVQHQKMLVAAWIVIGLVIVAFCLWLLGKSNQPGGLQVRSIAVLPFNSLTSDGQDEYL
                     GLGLADALITRLGNVRQITVRPTSAVRKYTRAEQDPVVAGRELKVEAVLDGNIHRVGE
                     RIRVTVQLVSVADGHHLWADKFDEQFTNILSVEDSISDRLATALALELTGEERKLLAR
                     RYTDNVEAYQLYLKGRYSLDKRTLEGVKKSIEYFEQAIESDQNYALAYAGLADAYSMP
                     DLSGLPPVDSYLKAKTAATKALEIDEGLAEAHLSLAYLRMRHEWDWRNCETELKRAME
                     LNPSIARTHHLYSIYCELMGRPDEAIREMKRAQELDPLSLVMNQNLGTRYYFARQFDQ
                     AIAQLHTTLDIDPGYGEAHIGLGQAYVQKGMYPKAILEFQKVTQSNDTPWVLGSLGHV
                     YGLSGNRAEAQKILEQLKAQSTRSYFPPEEIAKVYIGLGEKDQALVWLEKAFEKRSDH
                     LVFVKVDPVWDSLRADPRFTSLMQRIGLTL"
     gene            3169..3747
                     /locus_tag="PKZ53_02490"
     CDS             3169..3747
                     /locus_tag="PKZ53_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39331.1"
                     /translation="MDLKSIFLAFSIALAVSLFPSQSLAQENLKQRPEGTWNVILTAD
                     GEGFQEKERYTFIAGKSRKEGSLIFSNEVDAIPPCGTDQGVWNQTGRGEFALTHGAFC
                     LDPETQSPGFAIKFRETITLDESGETFSGKGIYEVFDPQGTAVFSATYTLQGTRMQIE
                     RLPGASRLDGAAPVQPTTEPHFGWRTWSRAEK"
     gene            complement(3842..4765)
                     /locus_tag="PKZ53_02495"
     CDS             complement(3842..4765)
                     /locus_tag="PKZ53_02495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNJ39332.1"
                     /translation="MHILDAIGNTSLVRLQKVIPSNGAEILVKLEWENPTGSMKDRMA
                     NTVISRAEADGRLKPGGTVIEYTGGSTGASLALVCAAKGYRIKIVTSDAFSPEKLNQM
                     MALGAELTLVPSEGGLTTKKLILDMIETARQLSQEPNTYWTDQLNNHDSIAGYYGLGE
                     EIWNQTEGKVDAFIHSVGTGASSRGVATVLKRHNPAIKIVVVEPAESAVLSGGQPGPH
                     KIEGVGIGYTPPLWEPSLVDEIIPVKTDDAKEMTRRLAREEALFAGTSSGANVIAALQ
                     VAEQLGPGARVVTLMADSGLKYLNTDVYRRK"
     gene            complement(4807..5310)
                     /locus_tag="PKZ53_02500"
     CDS             complement(4807..5310)
                     /locus_tag="PKZ53_02500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ39333.1"
                     /translation="MCIEILPALHEQEPILTNLLELYVYDFSEFMDLKLDAVGRFGYP
                     QLPLYWTEPHRFPFLIAVDGHWAGFVFVHKGSRISGDENIWDMAEFFILRGYRRLGIG
                     KQVAAEIWKKFPGTWEVRVIDQNQKAIAFWERAIADFTGTTVDPIPFKNDGKSWHVFS
                     FDSKHPA"
BASE COUNT         1285 a         1412 c         1265 g         1353 t
ORIGIN      
        1 aatcattgca tcttcgagag gctaaattgc tgataaaagg tatttccctc agcccttagc
       61 ccgatacccc agccccaaat ggtataacac tcgcgcgaac ctgccatata ataatcagac
      121 gctctcaaat aaccatagaa ttttagaaaa agatttcggt tctgaagtgc cactttgtgg
      181 cttcttattc tgaaaatcca tcctgtgtgg catccacagt caggcttcgc tttgcttcaa
      241 gccgggcttt gacttccggc cactcagatt cgatgatgct caaagagacc gagtcccgaa
      301 cccgaccatt gggcatgatc atatgattgc ggaggatgcc ttcttccctg gcgccaagtc
      361 gcacaatggc tgtccgcgac tggtgattga gcacatcggt aatgaacgcg acccgaatgc
      421 acttccaaac ctcaaaggcg tgggtcaaca acaacaattt ggtttcggtg ttcacgctgg
      481 tgcgttgggc actcgccgcc agccaggtgt atccgatttc cacgccccgg ttattctggt
      541 caatattgta aaaccgggtg cttccaacaa tttgacctga tgatttttgg acaatgacga
      601 acggaagctg aattccctgg gcctgtccgt ggagtgcttc cgcaatatag gccgccatgt
      661 tggtttgatc aggcacgatg gtcaccgtcg agttccacaa ttcgccatca ctggcagccg
      721 cgatgagcga ttcctcatgg gttggtgaaa ggggctccag tcggacgtgc tggccttcga
      781 gaattactgg cgaaatgtcc attgtacctc ctggatactg accaaatcag agtgtaaggc
      841 cgatacgttg catcagactg gtaaatcgtg ggtcagcgcg aagactgtcc cagaccgggt
      901 ccactttaac aaaaaccaga tggtcagaac gtttctcaaa ggctttttca agccatacca
      961 acgcctgatc cttttccccc agcccaatat aaacttttgc gatttcctct ggcggaaagt
     1021 aactccgcgt tgattgcgct ttcaactgct caagtatctt ctgcgcttca gcccgatttc
     1081 cggatagtcc ataaacatgg ccaagcgacc ctaaaaccca cggcgtgtcg tttgactgag
     1141 ttaccttttg aaactccagg atcgccttgg ggtacattcc cttttgcacg taagcctggc
     1201 ctaacccaat gtgggcttcg ccatagccag ggtctatgtc aagcgtcgtg tgtaattgcg
     1261 cgatggcttg atcaaactga cgcgcaaaat agtaacgagt cccaaggttt tgattcatca
     1321 ccagtgaaag tggatccagc tcctgggcgc gtttcatttc tcgaatggct tcatctggcc
     1381 ggcccattaa ctcacaatag atcgagtata aatggtgagt cctggcaatg ctggggttga
     1441 gttccatggc acgtttgagt tcggtttcgc aattcctcca atcccattca tgccgcattc
     1501 tcaggtatgc cagcgagagg tgagcctcag ccagcccttc gtcaatctca agcgccttcg
     1561 ttgcggctgt tttggctttc aggtaggaat caactggcgg aagtccagag agatcaggca
     1621 ttgaatacgc atcagccagt ccggcatagg ccagtgcata gttctgatca ctttcgattg
     1681 cctgctcaaa atactcaata cttttcttca ctccttccag ggtccgctta tccaaggaat
     1741 aacgaccctt gagatagagc tggtacgcct caacattgtc ggtatatcgg cgtgccagta
     1801 gcttgcgttc ctctcccgta agttccaggg caagcgccgt tgccagtcgg tctgaaattg
     1861 agtcttccac cgacagaatg tttgtaaatt gctcatcaaa cttgtcagcc caaagatgat
     1921 ggccatcggc cacgctcaca agctgcaccg tcactcggat ccgttcgccc acgcggtgta
     1981 tgtttccatc caacacggct tcaaccttca actcccggcc agccacaacc ggatcctgtt
     2041 cggcacgggt gtatttgcgc acggcactgg ttggccgcac agtgatttgc ctgacattcc
     2101 ccaaccgggt gatcaaggca tcagccagcc ccaatccaag gtattcatcc tgaccatctg
     2161 aagtcagcga gttaaatgga agaacagcga ttgacctaac ctgtaaacct ccaggctggt
     2221 ttgacttgcc caacagccac agacagaaag ccacaatcac cagcccgatc acaatccagg
     2281 cggcgaccag cattttttga tgctggactg gggaacccaa ttcaggagcg gcgacaggga
     2341 gagccgcttc tgtgtcttca ggttgagtct ctcgttcctg cctgacctcg tcttgattct
     2401 cttcaacaac gatggtggcc tgggggtgtt cttcgacggg caggtcagcc ccttcttcat
     2461 tccagacctc ctcgacacca gcgataaacc gatatccccg tctcggaagg gtttcgatgt
     2521 atttttgctg gctggaaggg tcagtcagaa ccttcctcag aatgtaaata ttttgagtga
     2581 gattggattc ttcgactacc gtgtcaggcc agactttgtc caggatttca tttttctcaa
     2641 gcacccttcc actattttcg accagcagca aaagggtatc gaacgtcttt ggttgcaacg
     2701 ccactggctc tccgtctctg agtaaaaggc ggttcccaat gtctatcgag aacaatccaa
     2761 actcatagaa gtgctttatt tgcttattca tgcgtatctt gagagattct tgagaaattc
     2821 ttgagcagcg tcttgaagat tttttcacca cccctaatcc atcatccgtc acaggttgag
     2881 ttgatgattc tcagccagtc ctgaccaaag aaactgaatg taccatcctg aattgggtgg
     2941 cgcaacaacg gctcatacac ctcaactccc cctgaaatat ctatgggcag aaaggaaaca
     3001 gattgagcaa accagtaagc aaacttagag tcaaaaccag ataaacccaa tttttttcaa
     3061 tattttcaat acctgttgcc atgtgacggt acacttttta atggcggcaa ccggcttttt
     3121 attggcattc gaaatttaca aaccaccagt ctatgaggag tacatattat ggatttgaaa
     3181 agtattttcc tggctttttc cattgctttg gcagtaagcc tcttccccag tcagtcactg
     3241 gcccaggaaa atctcaaaca gaggccagaa ggcacctgga acgtcatcct gaccgcagat
     3301 ggagaaggct ttcaggaaaa agaacgctat acatttatag caggtaaatc cagaaaggaa
     3361 gggagcctga tcttttcaaa tgaagttgat gccatcccgc cctgcggtac tgatcaaggc
     3421 gtgtggaatc aaactggccg tggtgaattt gcgctgacac acggggcctt ttgtcttgac
     3481 ccggaaaccc agagcccagg gttcgcgatc aaattcaggg agacaataac gcttgacgag
     3541 agcggcgaaa cattttctgg gaaaggtatt tacgaggtat ttgatcccca aggaactgct
     3601 gttttctctg cgacttacac gctgcagggc acccgcatgc aaatcgagcg gctgccgggt
     3661 gcgtcacgat tggacggagc ggcaccagtt cagccgacga cagaaccgca ctttggctgg
     3721 cggacctggt ctcgcgctga gaaatgagct tcgtgagatc aagaacagcc aggaaaactg
     3781 aaccattcct ggaaactgca tccagtgctg gttggacgct ggtcccgcca gcaccgtgac
     3841 tctatttcct tcggtataca tcggtgttga ggtatttcag gccggagtca gccatgagtg
     3901 tgacgactct ggcgccgggc ccaagctgtt cggcaacctg aagtgccgca atgacattgg
     3961 cccccgaaga tgtcccagcg aagagtgctt cctcacgtgc aaggcggcgt gtcatctctt
     4021 tggcgtcatc ggtttttact ggaataatct catccaccag cgatggttcc cacagcggtg
     4081 gcgtgtagcc aattccgacg ccttcgattt tgtgcggtcc tggttgtcca cctgacaaca
     4141 cggctgattc agcaggttcg accacaacta tcttgattgc cgggttgtgt cgtttcagca
     4201 ccgtggcaac tccacgcgaa gacgcgcctg tcccgacgct gtgaataaaa gcatctacct
     4261 tcccttcggt ttgattccag atttcttcgc ccaatccata gtagcccgca atgctgtcgt
     4321 gattgttaag ctggtcggtc cagtacgtat tcggttcctg gctcaattga cgggcggttt
     4381 cgatcatatc gaggatcagc tttttggttg tgagcccacc ttcgctcgga acaagcgtca
     4441 gttccgcgcc caacgccatc atttgattga gcttttctgg gctaaacgcg tcagaagtca
     4501 caattttgat ccggtagcct ttcgcagcac acaccagagc cagcgatgcc ccggtgcttc
     4561 cgcctgtgta ttcaatcacg gtgcctccgg gtttgagtcg tccatcggct tcagcccgcg
     4621 aaatcacagt gtttgccatc cgatctttca tactgccggt tggattttcc cattcgagct
     4681 tcaccagaat ttccgcccca ttggaaggaa taactttctg aagtcgaaca agcgatgtat
     4741 ttccaattgc atcaagaatg tgcatagggt ggggctcctt gttgtgacgg ggttctggat
     4801 aaggtgtcag gcagggtgtt tcgagtcaaa tgaaaagaca tgccagcttt tcccatcatt
     4861 cttgaaaggg attgggtcaa cggtggtacc ggtaaaatca gcaatggcac gttcccaaaa
     4921 agcgattgcc ttttgatttt ggtctatcac ccgaacttcc cacgttcctg gaaacttttt
     4981 ccagatttct gctgccacct gttttccaat gccgagccga cgatagccgc gaagaataaa
     5041 aaactccgcc atatcccaga tattttcatc accggaaatt cgagaacctt tgtgaacaaa
     5101 cacaaatcca gcccaatgac catcaacggc aatcaggaac ggaaaccgat ggggttctgt
     5161 ccagtataac ggaagctgcg gatacccaaa tcgcccaacg gcatcaagct tcagatccat
     5221 aaactcacta aaatcataca catacaactc aagtaagttt gtaagaattg gctcttgttc
     5281 gtgtaaagct ggaagaattt caatgcagat gggtg
//
LOCUS       DAOQDO010000098         5296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_9922_length_5296_cov_2.907270, whole genome shotgun sequence.
ACCESSION   DAOQDO010000098 DAOQDO010000000
VERSION     DAOQDO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9922_length_5296_cov_2.907270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..2124)
                     /locus_tag="PKZ53_02505"
     CDS             complement(295..2124)
                     /locus_tag="PKZ53_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNJ39334.1"
                     /translation="MALPLTLKQLSQPSLPTGMFKTVSVSCLVLGLTLTSTSLPTLAI
                     NTRDLTGRSVASAQSSRYRSYEIPVGAVLKLRLNSQLTSKTNRVGDTFTATVFEPYLI
                     DGKAVVPQGTQVEGHVSSVEPAQRDRSGTIAVDFDYLIFENGKKLDISQVSELTSLKR
                     DEKQQIDAEGKTSGGSTTKRKIIFTGGGAGAGAVIGAIAGGGKGAAIGSAIGAGAGIL
                     TSLLLKGEEAVVKSGQEFGLEFLQKARVSDEYLTARSTTDTTDRDRDTRDRDTTDRDT
                     RDRDTRDRDTRDRDTRDRDTRDRDDRDRYTGSQRDPYQEYTDPQFIRRAQLELRSRGF
                     YTSTIGTTMTSGVRSALSRFQRDQRLEETGKLDLPTAQALDLVDRDGNEIRLVKVVAA
                     RAVRQADRSIQVVMDTEVPSGGWDLYGDYEIRTDKLEVWARGVAPAGAASQVITKGTI
                     EVVPREDVSRIQTVLIHTDTKDLTVAVEDMQTGIAKSLKATADRMLSTLRTQFRSEQR
                     GATRTAPTTISWRLNENEARLYSAVVSLAEATRFYAELVESRATEDAQRGAAEALTRG
                     LNRVDRVMRSGRVGDRIQRDVDDFDKAAQQLTDYFRIDTRGND"
     gene            2531..2905
                     /locus_tag="PKZ53_02510"
     CDS             2531..2905
                     /locus_tag="PKZ53_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNJ39335.1"
                     /translation="MKNLMTRLFAVLALSIVVSASALAGETKKDVSFSSDVTINGTLV
                     KKGKYQVVYDDKTGEMSIRKGKEELVKATTRTEDRQKKAIRLEMLYTQNGENRSLQGF
                     AFEGSNQNIVINGNAQAATPQQ"
     gene            3388..4509
                     /locus_tag="PKZ53_02515"
     CDS             3388..4509
                     /locus_tag="PKZ53_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNJ39336.1"
                     /translation="MKEIQTIVAAYREHLRQDSGIAALATVVSVKGSVYRRPGARMLI
                     STHGHSLGAISGGCLEGDVVERASQVMISGQSQLVVYDTTSDGDTVWGLGMGCNGEVQ
                     VLIEPVSPTAAPHPITVLETLLSQSLPLVWATVVKTTDVNCVSVGSHLLLHRDGVVHS
                     SIPNQALAKLLVADAEEVLRHQKSEFKASVVEGVTIQVFLEFLEPPQPLVIFGAGYDA
                     LPLVALASELGWQVTVIDHRPAFARADRFPLANQVIHSHPETISDHVKLTPRTVAVVM
                     THNYQRDGEVLKALLPFPLPYIGMLGPKRRTDALLNDLQEAGVEITPGQRQRIFGPAG
                     LDIGAETPEEIALSIIAEIRAVITGHQAGFLRQRTGPIH"
     gene            4754..>5296
                     /locus_tag="PKZ53_02520"
     CDS             4754..>5296
                     /locus_tag="PKZ53_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39337.1"
                     /translation="MTKQPLRFEPNRGQTDSNVEAYCRAAQYIAFFTGIEIIFGFPEV
                     PFSKDKAHLFQKQPPTPCQKVASLPVKLTFRGASTSRKLKLEQPLTGDSHYFLGTDRQ
                     TWVRDVPGSAVLRYEDLYPGIDLVGYGQPDALEYDFIVSPHANPAQIVLEISGTNQIN
                     LNQNGDIEIETPTGTLVHHQP"
BASE COUNT         1289 a         1263 c         1371 g         1373 t
ORIGIN      
        1 tgaagaggtc gggctgaaga cttcgggctt ggggctgaag aagtcgggct gaagacttcg
       61 ggctgggggc tgaagacttc gggctgaaga ggtcgggctg aagacttcgg gcttggggct
      121 gaagaagtcg ggctgaagaa ttgggtttct ttcatccctc agccttcagt tgtcccaggc
      181 ccgaagtttt taactctact tgctactcat cccatttcgg aatggagtcc tcacgttaga
      241 aaatagtcaa gtagttcaat caaataaacc tggtgaacta cggactctgg actactaatc
      301 atttccacga gtatcaatcc ggaaatagtc agttaattgt tgagccgctt tatcaaaatc
      361 atccacatcg cgctggattc gatcaccaac gcgaccagaa cgcatgaccc gatcaacccg
      421 gttgaggcca cgggtcagag cttcggcggc cccgcgctgg gcatcttcgg tggctcggct
      481 ttcgaccagt tcggcataaa accgggtggc ttctgccaga ctgaccacgg ctgaataaag
      541 ccgggcttcg ttttcattca agcgccagga aatagtggtt ggggcggtac gggtggcgcc
      601 gcgttgttca cttcggaact gagttctgag ggtgctcaac atgcggtcag ccgtagcctt
      661 aagtgatttg gcgatgcccg tttgcatatc ttcaactgcc accgtcaaat ctttggtgtc
      721 agtgtgaatc aacacggttt ggatccgtga cacatcttca cggggaacga cttcgatggt
      781 gcctttggtg atgacctgtg acgcggcgcc ggctggtgcc acaccacgcg cccagacttc
      841 gagcttgtcg gttcggattt cataatctcc atacagatcc catccgccgg aaggcacctc
      901 ggtatccatc acgacctgga tactgcggtc agcctggcga acagctcgcg cagccacgac
      961 cttgacgagt cgaatttcgt taccatcgcg atcaaccaga tcgagcgcct gagcggttgg
     1021 cagatcgagt ttcccggttt cctcaagccg ttgatcacgc tggaatcggg aaagagccga
     1081 ccgcacaccg gaagtcattg tggtcccaat agttgaggtg taaaaaccac gtgaccgaag
     1141 ttcaagttgc gcccgccgga taaactgggg atcggtatat tcctgatatg gatcacgttg
     1201 agacccggta tagcgatccc ggtcatcccg gtcgcgggtg tcgcgatcac gagtatcgcg
     1261 gtcacgggtg tcgcggtcgc gggtatcgcg gtcacgagta tcccgatctg tggtatcccg
     1321 gtcacgagtg tcgcgatcac gatcagtggt gtctgtagtt gatcgggcgg tgaggtattc
     1381 gtccgaaact ctggctttct ggaggaattc gaggccaaat tcctgtccgc tcttaaccac
     1441 ggcttcttcg cctttgagca aaagggatgt cagaatcccc gccccagcgc cgatggcact
     1501 gccaatcgcc gcacctttcc caccgccggc aatcgctcca atgactgcgc ccgccccggc
     1561 gccaccgcca gtaaaaatga ttttgcgttt ggtggtggag ccgcctgagg tttttccttc
     1621 agcatcaatt tgttgttttt cgtcacgttt aaggctggtc aactcagaaa cctgactgat
     1681 atcgagtttt ttgccgtttt caaaaatcaa atagtcaaaa tcaaccgcga tggtgccaga
     1741 gcgatcccgc tgggctggct caacgcttga aacgtggcct tcaacttgag ttccttgtgg
     1801 gacaactgct tttccgtcaa tgagatatgg ctcaaatacg gtagcggtga aggtatcacc
     1861 gacccggttt gtttttgaag tgagttgtga attcagtctg agtttcaaaa ccgcgccaac
     1921 cgggatttca tagctccggt agcgtgaact ctgggcgctg gccaccgatc ggccagtcaa
     1981 atccctggta ttgatggcca gcgttggaag tgaggttgat gttaaggtca gaccaagtac
     2041 cagacaggaa accgaaacgg ttttgaacat tcccgtcggt aaagatggtt gagaaagctg
     2101 cttcaatgtc aatggcaaag ccataacttc cctcctatga agtggttcca agaaaatcat
     2161 gagttaaaga tgacgttgtt agactccctc ctggtttggt gaaaaccaga ggatgccgtg
     2221 cccagccgaa gtctgattgt ggatgagacg gtaagtgaaa tggtacagaa atccagacga
     2281 gggtattgcg aatgatgctg agagcatagg cgaaagatgc cttatcggct aggtctttat
     2341 gggttgaact gaaatgccgt gcttcattca ttagatttat tttattattt tatttgaata
     2401 aaaattttac ggattcgtaa ttttctggaa cttttgtttt gttaccacgt ttgaggcgat
     2461 gctgacacaa taaatcaatt aaattatttg acgacccgag aaaattcatc aactgtgagg
     2521 agacactgac atgaaaaatc tgatgacccg tttgtttgct gttctggccc tttcaatcgt
     2581 agtgagcgct tctgctttgg caggcgaaac aaagaaagac gtttctttct caagtgacgt
     2641 aaccattaat ggaacccttg tcaagaaagg caaataccag gttgtgtatg acgacaaaac
     2701 tggtgaaatg tcgatccgca aaggaaaaga agaactggtg aaagccacaa cccggactga
     2761 agaccgccaa aagaaagcta ttcgattgga aatgctctac acgcaaaacg gtgaaaaccg
     2821 tagcctgcaa ggatttgctt ttgaaggctc aaatcaaaat attgtcatca acggcaatgc
     2881 tcaggcggca accccacagc aataatcaga aactttttga gccgattcaa aaaccccagg
     2941 tacaaaattt tgtacctggg gtttttcttt tgtttttaaa tatgtattta gataaatcaa
     3001 aataataaaa gaatctggtt aaataattct ttaattattt tgaatttatt ttgaatattt
     3061 tgaatattta ttttgattaa attacggatt tcttctgata tcaggattcc gactgtggtg
     3121 tgaatagaag aaacaacacc tgcaacgtcg tgcctttgga aaggcgtgag atgaagaatt
     3181 ttgattgaag ctggattttt ggggtgattt agtgcctgtg gtacgcaatg acccggaaaa
     3241 tgttccgtcg cctgccggtt gttgagggtt ggcctgacct ggaactttta caaaaaaaca
     3301 atcatttttg accaaagccc tcctataatc tgccgactcc ccgattcaaa atttcactgt
     3361 ctttgatgtt ctcatcattc aaaaaccatg aaagagattc aaacgattgt agctgcgtat
     3421 cgtgaacacc tgcgtcagga ttctggaatc gccgcgctgg cgacggtcgt cagtgtgaaa
     3481 ggctcagtgt accggcgacc aggggcccgg atgttgattt caacccacgg gcacagcctg
     3541 ggtgctatta gtggtggctg tcttgaaggc gatgtggttg aacgtgcctc tcaggtgatg
     3601 atctcaggcc aatcccagct tgtggtctat gacaccacgt ctgatggcga cacggtgtgg
     3661 ggacttggga tgggctgcaa cggtgaagtt caggtgttga ttgaaccagt ctctccgact
     3721 gccgctccgc atccgatcac agtgcttgaa accctgttga gccagtcgct ccctctggtc
     3781 tgggcaactg tagtcaaaac aacggatgtc aactgtgttt cggttggttc acaccttctg
     3841 ttgcaccgtg atggagttgt gcacagttca attcccaatc aggcactggc caaattgctg
     3901 gtggctgatg ctgaagaggt ccttcgccac caaaaaagtg agttcaaggc atctgtggtt
     3961 gaaggggtga cgattcaggt gttccttgaa tttttagagc caccgcaacc gctcgtcatt
     4021 tttggcgccg ggtatgatgc cctgcctctg gtcgcactgg ccagcgagct tggctggcag
     4081 gtgacggtga ttgatcatcg tccagctttt gccagggcag atcgctttcc actggccaat
     4141 caggtgattc atagccatcc tgaaaccatc tctgatcacg tcaagttgac gccacgcaca
     4201 gtcgccgtgg tgatgacgca taactaccag cgtgacggtg aagtcttaaa agccttgctg
     4261 ccatttcctc tcccctacat tgggatgctc ggaccaaaac gccggacgga tgccttgctc
     4321 aacgatcttc aggaagcagg tgttgaaatt acgccaggcc agcgacagcg aatttttggt
     4381 cccgccgggc ttgatattgg cgccgaaacc cctgaagaaa ttgcactttc gattattgct
     4441 gaaattcggg cagtgatcac tggccaccag gctggttttc tgcgccaacg aactggaccg
     4501 atccattgaa aaaccagccc gaagttgtca gcccgtttcc ttcaatccga cctttttgct
     4561 tcatcctctg cgtatgaact cattttttcg gacaagaact cagcttctgt gttgttttct
     4621 gatggtgttc tgcggaagtg tggggcaatt tcggccaggg ttcggggttc agagcagtgg
     4681 aaagaaggag tctgctcaaa ccgtgaaacc ggtgtcaggg tgggcggaca ggttgaaaca
     4741 agaagtttca tttttgacta agcaaccgct tcggtttgaa ccaaatcgcg gccagacgga
     4801 ctcaaacgta gaagcctatt gccgggcggc acagtacatc gcctttttca ctgggattga
     4861 aattatcttt ggatttcctg aagttccttt ttcaaaagat aaagctcacc tttttcagaa
     4921 acagccaccg acaccttgcc agaaagttgc cagccttcct gtgaagctga cctttcgcgg
     4981 tgcttcaacc agtcgaaaac tcaaacttga acagccactg actggagata gtcattattt
     5041 tctggggaca gacaggcaaa catgggtgcg tgatgttcct ggaagtgctg tgctgcgata
     5101 tgaagatctc taccccggaa ttgatctggt tgggtacgga caacctgacg cactggaata
     5161 tgatttcatt gtgtcgccac acgccaatcc agcccagatt gttttggaaa tttcaggtac
     5221 gaatcagatc aatttgaatc agaacgggga catcgagatt gaaaccccca cggggacgct
     5281 tgttcaccac cagccg
//
LOCUS       DAOQDO010000099         5266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10037_length_5266_cov_2.710996, whole genome shotgun sequence.
ACCESSION   DAOQDO010000099 DAOQDO010000000
VERSION     DAOQDO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10037_length_5266_cov_2.710996"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PKZ53_02525"
     CDS             complement(<1..752)
                     /locus_tag="PKZ53_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNJ39338.1"
                     /translation="MKSFRKILFWCHLLAGVIAGVVVFVMSVTGVLLAFEAQITRFAE
                     RDQRVVQPPSANAVRMSPQALLAKVQADNPTVKPAGITVEADPTAAVSVSLGRDGTLF
                     VNPYTGASLGTGSKGVRNFFHEITDWHRWLGAQGESRSIGRAITGACNAAFLFLAISG
                     IYIWWPKKWTRQHLAAITLFRSGLKGKARDFNWHNVIGIWCVLVLIVLTATGMVISYQ
                     WANNLIYTITGSPRPGPPPGAGGPGAGGPGGPG"
     gene            complement(1050..3614)
                     /locus_tag="PKZ53_02530"
     CDS             complement(1050..3614)
                     /locus_tag="PKZ53_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39339.1"
                     /translation="MRTLFHSIDLFATLPQGFLPSQARLMSVRYTTALSLAVLLMVLM
                     WLSPAMQAQSLTGLLQGTVTDQAGAVVQGARIGLRSASAQPVTQETVSDSQGQFSLKN
                     LPAGEYTLIIEKKGFQPRQQVIAMTSDLAPMTLTLTPETLSEVVMVTDNSIAVESTFK
                     LETSIHETPRAVTIIDSTRIREQNFRFVNDAFAYVPGVAVNSYRTGGYHFYARGYRML
                     PDDTRVDGFAGINAGGRYGATLFGVEQAVLLRGPAGLLYGSSGAPGGMINLITKKPQP
                     VQTTRIDLRTGGFAGNGVGLTERGFGGLDIDTTGPVGKTDRVFYRALFTIENMNYFTN
                     NVLDRNRYAQGSLTFKLDQEGRYTLTPMFQWTRFNRPAGGGIVISPTTSLSTNDKISG
                     PVNTRDLSPLDVNLSFGGGIDEGRQAGFDFRAVPVHALRINLGYRFIGLDTSLNQFTP
                     VASTPTQIDILQKQNLVQRQQTKSETDRLYHNFDFNTAYEFQPTSWWKNTVQVGGYAR
                     LSRTRTTTNAGPLPAPQSFINIYTGQTTTPLVDNYPALKWGDWMDTTFWNTYVQNRAA
                     LANGKVIFSFGLGYGQNHPGGVPVRKGELTPNLSLVYNVDHDLALFFSYATSYNPADA
                     NAEDITGKRNTFDPSIGRNYEFGAKYDLPNRRASATLSFFHNEVTNALVQSGPNDLNP
                     NGNRYFVEAGSRRARGIELSTEFQPIRDLRVTAAVSYLDAIYTGDGPASAAPTNAIPG
                     SRAEKSPKWSYSVWSRYDRTEGKFQGFGAGLGLIWQDERLGGNGARTPTAPDPLLLPA
                     FTRLDAALFYRWNEHIDFALNFENLTDRVIFVSGTVGSGLEIAPPRTVSFRMGYRF"
     gene            3882..4169
                     /locus_tag="PKZ53_02535"
     CDS             3882..4169
                     /locus_tag="PKZ53_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39340.1"
                     /translation="MSKKAYFEIDFQNRANYAGHRPALSTTLLIFFHRNQLYHTVISL
                     NSSSLANLLPLLNNPGQNAKIIASDGQPANQLKNLGVPETLNPEPCPSLVL"
     gene            4166..>5266
                     /locus_tag="PKZ53_02540"
     CDS             4166..>5266
                     /locus_tag="PKZ53_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ39341.1"
                     /translation="MTQPLPPPAAIVRPPAEILYAQELNALAANDKSPHPPGWKLSPR
                     LVREFILGSDGKAYPHIWEGQPTETVIQEKFYGDDALIDRAIITLAGNRGLLLVGEPG
                     TAKSMLSELLTAAISGTSTNTIQGTAGTTEDHIKYTWNFALLLAEGPSLRALVPSPIY
                     TGLKEGRLVRFEEITRCPPEIQDSMVSILSDKVLHIPELDEDHAVLFARKGFNIIATA
                     NLRDRGVNEMSSALKRRFNFETVHPIASKELELKLVLEQTQLLLAEAEATVQIETDVI
                     DLLVTTFHDLRQGRTADGTAVERPSTVMSTAEAVSVGLSAGLDACYFGDGQLAPEHIA
                     RNLIGTVLKDSSEDAKKLKHYFDVVVKARAKNS"
BASE COUNT         1303 a         1486 c         1324 g         1153 t
ORIGIN      
        1 cctgggccgc caggtccacc ggcacctggg ccaccagctc ctggaggcgg gcccggacgc
       61 gggctgcccg tgatggtata gatcaaatta ttggcccact ggtacgaaat caccattccg
      121 gttgccgtca gcacaatcaa caccagcaca caccagatcc caatcacatt gtgccagtta
      181 aaatcacgcg ccttcccttt caaccctgac ctgaacaacg taatcgcggc cagatgctgg
      241 cgagtccatt ttttcggcca ccagatgtaa atgccactga tggcaagaaa gagaaacgcg
      301 gcattacagg caccagtaat cgctcgacca atggaacggc tttcaccctg cgccccaagc
      361 caccggtgcc agtctgtgat ttcgtggaaa aaattgcgaa cccccttcga ccccgtaccc
      421 agcgaagcac ccgtgtacgg attgacaaac agtgtcccat cacgtcccag cgagacagag
      481 acggcggctg tcggatcggc ttcaaccgtt attccggctg gttttaccgt cggattgtca
      541 gcctgaactt ttgccagaag cgcctgcggg ctcattcgta ccgcatttgc ggaaggaggt
      601 tgaacaactc gctggtcacg ttcggcaaac cgggtaatct gggcctcaaa agccagcaaa
      661 acccccgtca ccgacatcac aaacaccacc acaccggcaa ttacaccggc cagcaaatga
      721 caccaaaaga gaatttttcg aaaggacttc atacgcttta tgtaccatca gaacaaaatt
      781 acggctttta ctgaaaaaga aagcaggtca atgcgcctcc attgaagcac attgaccctg
      841 aatagacgat gaatgtctga gggctagggg ctcagggctg aagacatcgg gctgggggct
      901 gagggcttgg ggcaattgaa gggatgaggg atgtgaggga tgaaagaaac ccagttcttc
      961 agcccgaaaa tcttcagccc tcagcccgac ttcttcagcc caacttcttc agcccgtctt
     1021 cttcagcccc aagcccaatg tcttcagccc taaaaccgat atcccatccg gaaactgacc
     1081 gtgcgcggtg gtgcaatttc aagacccgaa ccaaccgttc cgctgacgaa aatcacccgg
     1141 tcggtcagat tttcaaagtt aagggcaaag tcaatatgtt cgttccagcg gtagaaaagt
     1201 gcggcatcca gtcgcgtaaa cgctggcaac aagagcggat ccggagcggt tggagttcgc
     1261 gccccgttgc ctcccaggcg ttcatcctgc cagatcaatc ccaaaccagc accaaaaccc
     1321 tggaatttcc cttcggtccg atcatagcgg ctccagacgc tataggacca cttcggagat
     1381 ttttccgccc gtgaacccgg aatggcattg gtcggtgccg cactggctgg cccgtcaccc
     1441 gtataaattg catccaggta actcacggcg gcggtgacac gcaaatcccg aattggctga
     1501 aattccgtgg acagttcaat accgcgtgcc cgacggcttc cagcttcgac aaaatagcgg
     1561 ttgccatttg ggttgagatc gttggggcct gactggacca gggcattggt gacttcattg
     1621 tggaaaaagc tcagggtcgc ggatgcccgg cggtttggca gatcatattt cgccccaaac
     1681 tcatagttgc gaccaattga agggtcaaac gtgttgcgct tcccagtgat gtcttcggca
     1741 ttggcatctg ccgggttata actggtggca tagctgaaga acagcgccag atcgtgatct
     1801 acgttgtaaa ccagtgacag atttggcgtt aactcccctt tgcgaaccgg aaccccacct
     1861 ggatgattct gaccataccc taacccaaat gaaaagatga cctttccatt agccagcgcg
     1921 gctcgattct ggacgtaagt attccaaaat gttgtgtcca tccagtctcc ccacttgagg
     1981 gccgggtagt tatcaaccag tggtgtggtt gtctgaccag tataaatgtt gatgaaggac
     2041 tggggcgccg gcaacggtcc ggcattggtc gtggttcggg tgcgcgacaa ccgggcgtat
     2101 ccgccaacct gaactgtatt tttccaccag cttgtcggct gaaactcata cgccgtgttg
     2161 aagtcaaaat tatggtagag ccggtcagtc tcgcttttgg tctgctggcg ctgaaccagg
     2221 ttctgttttt gcagaatgtc aatctgggtc ggggtgctgg caaccggagt aaactggtta
     2281 aggctcgtat ccagcccgat aaaccgatac cccaggttga ttcgcagcgc atgaactgga
     2341 accgctcgaa aatcaaaacc agcctgacga ccttcgtcaa tgccgccccc aaacgataaa
     2401 ttgacatcga gtggcgacaa atctctggtg ttgaccgggc cgctgatttt gtcattggtt
     2461 gaaagcgatg ttgtcggaga aatcacaatt cctccgcctg ctggccggtt aaaccgcgtc
     2521 cactgaaaca tcggcgtcaa cgtatagcgg ccttcctgat ccagtttgaa cgtcaacgac
     2581 ccttgagcat aacggtttcg gtcgagaacg ttgttggtaa aatagttcat gttttcaatc
     2641 gtgaacaacg cacgataaaa cacccggtcg gtctttccaa ctggaccggt ggtgtcaatg
     2701 tcgagaccac cgaagccacg ctcagtcagc ccaactccat ttcctgcaaa accgccggtt
     2761 cgcaaatcaa tccgggtcgt ttgaaccggc tgtggcttct tggtaatgag gttgatcatt
     2821 ccgcctggcg cgcccgacga cccatagagc aatccggctg gaccgcgtaa caacacggcc
     2881 tgttcgacgc caaacaatgt tgcaccatag cggcctccgg cgttgatgcc agcaaagcca
     2941 tccactcgcg tgtcatcagg caacatgcga tacccgcgag catagaagtg atacccgccc
     3001 gtgcgatagc tgttgacggc aacacctggc acataggcaa acgcatcgtt gacaaagcgg
     3061 aaattctgtt cacggatgcg tgtcgaatca atgatggtta cggcacgtgg cgtttcatga
     3121 attgaagttt ccagcttgaa ggtggattcc acggcgatgc tgttatccgt gaccatcacc
     3181 acttcgctca gtgtctctgg ggtgagcgtc aaggtcatcg gcgccaggtc agaggtcatc
     3241 gcaatcactt gctggcgcgg ctgaaaccct tttttctcaa tgatcagtgt gtactcaccg
     3301 gcgggcagat ttttgaggct gaattgccct tgactatcag aaaccgtttc ctgagtcacc
     3361 ggctgggctg aggctgaacg aaggccaatt ctggccccct ggaccacggc tccagcctgg
     3421 tcagtcaccg tgccttgaag taaaccagtc agcgattgcg cctgcattgc tggcgaaagc
     3481 cacatcagaa ccatcaaaag cactgccaac gacagcgcag tggtgtatcg aacagacatc
     3541 aaacgtgcct gagagggtaa aaacccttga ggtaaggtcg caaaaagatc aatcgaatga
     3601 aaaagtgtac gcatagcatc catccagtca ggtgacatcc ggccaatccg gaggtccaag
     3661 acacacgcct gccttccgcc ttggagcaag acgttatgtg aaagttaaaa acagtgggct
     3721 gaatggagag ctgtcaccgg ctgccgccaa cctgaagagg ataaaatgga tggaggtatg
     3781 ctagaatcag gacgcactca ccaaacagaa acaccgcaca gcaatggtga ttggtttgtg
     3841 caatagagcc tctggcgttg ccgcgccaga ggctttttgc attgagtaaa aaggcttatt
     3901 ttgaaattga ctttcaaaat cgagccaact atgccggaca tcgccctgct ttgtcaacca
     3961 ccttgttgat tttctttcat cgaaatcagc tttatcatac cgttatctca ttgaattcaa
     4021 gcagcttagc aaaccttctt ccacttttga acaatccagg gcagaatgca aaaatcattg
     4081 cttccgatgg acaaccagcg aaccaactga aaaatttggg tgttcccgaa accctgaacc
     4141 ctgaaccctg cccctcattg gtcttatgac tcaaccactg ccaccccctg ctgccatcgt
     4201 ccgcccacca gccgaaatcc tctatgctca ggaactcaac gcacttgcgg ctaatgacaa
     4261 atcgcctcac ccacctggat ggaagctttc accgcgcctg gtccgtgaat tcatccttgg
     4321 ttccgacggc aaagcctatc cccacatctg ggaaggccag cccacggaaa ctgttatcca
     4381 ggaaaaattt tatggggatg atgccttaat tgatcgggcg atcattacgc tggctggaaa
     4441 tcgtgggctg ctgctggtgg gtgaacctgg aaccgcaaaa tccatgcttt cggaactcct
     4501 cacggcggct atttcgggaa catcaaccaa caccatccag ggcacggcgg gaaccaccga
     4561 agaccacatc aaatacacct ggaattttgc cctgctcctg gccgaaggcc ccagcctgcg
     4621 ggcgttggtt ccgtccccga tctataccgg gttaaaagaa ggccggcttg tccgctttga
     4681 agaaatcacc cgttgcccgc ctgaaatcca ggattcaatg gtcagcattc tgtccgacaa
     4741 ggtgctgcac attcccgagc tggacgaaga ccacgccgta ctttttgccc gcaaagggtt
     4801 taacatcatt gccaccgcga atttacgtga ccggggcgtt aatgaaatgt cgagtgccct
     4861 caaacggaga ttcaactttg agacggttca tccgattgcc agtaaagaac tggaactcaa
     4921 actggttttg gagcaaaccc agctcctgct cgccgaagct gaagccacag ttcagatcga
     4981 gaccgatgtc attgacctgc tcgtgactac gtttcacgat ctgcgccagg gtcgcactgc
     5041 cgacggcacg gctgtggaac gtccctcaac cgtgatgtcc accgccgaag ccgtctcggt
     5101 tggattatct gccggactcg atgcctgcta tttcggagac ggccagcttg cccccgaaca
     5161 catcgcccgc aacctgattg gaacggttct caaagacagt tcggaagatg ccaaaaagct
     5221 caagcattac ttcgatgtgg tcgtcaaagc ccgcgcgaaa aatagt
//
LOCUS       DAOQDO010000100         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10074_length_5253_cov_2.954021, whole genome shotgun sequence.
ACCESSION   DAOQDO010000100 DAOQDO010000000
VERSION     DAOQDO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10074_length_5253_cov_2.954021"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKZ53_02545"
     CDS             <1..477
                     /locus_tag="PKZ53_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HNJ39342.1"
                     /translation="AYCGEIHDDLPDIGSDRPDLCWSIPGTEWSQRVDLTAETCVVDK
                     VHFLIRGVIELPIHEAPETFGFGVWVSLTREDLDTYLENPDTDEIGPFSGRLGTNIRF
                     YDEDTTSLQTLVHFLGHNARPIILLDPMTNHPLGIDQRNGISLSKAWEIAHFYGVE"
     gene            474..929
                     /locus_tag="PKZ53_02550"
     CDS             474..929
                     /locus_tag="PKZ53_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014232701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HNJ39343.1"
                     /translation="MKPTILALDLEGTLISNAVSQIPRPGLSAFLEQCHQLFSRIVMF
                     TSVDEIRFRQIAKRLVEDGETPDWFAEIEYITWTGPIKDLTFVPDATPAKIVLVDDFE
                     RYIHPEQKSQWIAIDCFQSPYSPDDTALEDCVQRLQAQVNAHAGSQSDD"
     gene            956..1639
                     /locus_tag="PKZ53_02555"
     CDS             956..1639
                     /locus_tag="PKZ53_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNJ39344.1"
                     /translation="MEEYLVEIKQFHKQSVEPYTGKPVGCIEREILLLEQKTGFALPE
                     AYRQFLRYMGKDTDGVFRGSGWFLGNIGLNDTLVPDLLEENAITFQLPEHYFLFFSHQ
                     GYMAAWFEVPRLNDNPPVFFFSEGEDWKGPQKIDTFTTFLFDDLKGLASLLPRLYRFG
                     EGVQKDEARAQNQARQYPILLKAAFRHLKKKQYAQALLFFDQAEAIKPLTEKEMDARR
                     VCEERLARG"
     gene            complement(1741..2214)
                     /locus_tag="PKZ53_02560"
     CDS             complement(1741..2214)
                     /locus_tag="PKZ53_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39345.1"
                     /translation="MLITIGIKQKPSWLKLKKFKILAGLAIFLVIGGLLVFWATWPHW
                     YFRTFDISVCYGQQMLASPVVYRSINDNYIIDLRDQFRVGDTYLYSPQAQEIRFFNQS
                     GFFTFWGIAFNRQNPPLSSPLGKSDVDPQISSTQDSIEFTSGQSVRIKIIKGKRL"
     gene            2369..2845
                     /locus_tag="PKZ53_02565"
     CDS             2369..2845
                     /locus_tag="PKZ53_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39346.1"
                     /translation="MRLRKRLTQAYVAALLHLSAWLARRERLNRITQSFMRFLAGIVI
                     RRKNISPQTTLSELGQAWQQAFPSVKHHPITKIDDTTVYGEIHTNCPLRGTGDVHACH
                     RMMEFDRTIARQAGGEFVVLRSQSEPGVTVCQIAMRLRGSDTSDLQPAHQPTHARS"
     gene            3020..3397
                     /locus_tag="PKZ53_02570"
     CDS             3020..3397
                     /locus_tag="PKZ53_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002784626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNJ39347.1"
                     /translation="MLQTYRDLEVWKKSIDLVESTYQLTKSFPAEEKFGLISQIQRAV
                     VSIAANIAEGYGRKHRKEYLHHLSIAKGSLLEVETHLIIAIRLHFTTRDDAKSVWHLC
                     QDVGRLLNKLVNSLNSKGLNPEP"
     gene            3453..4244
                     /locus_tag="PKZ53_02575"
     CDS             3453..4244
                     /locus_tag="PKZ53_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011049376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ39348.1"
                     /translation="MNFKSSFLRVGKVQLEYRWVGPPPDECPTLVFLHEGLGCVSLWR
                     DFPEQLFLQTGCGALVYSRAGYGKSDPASLPRPVWFMHDEAEILGVILEQTGIKKPIL
                     LGHSDGASIALIYAGGPQGSSVSGLILEAPHVFTEPYGLESIARIRELYRTTDLRQRL
                     ARHHGDNTNIAFWGWNDVWLHPAFQAWNIESVLPAITAPMLIIQGETDEYGTWRQVEA
                     IQHQSGGKVQTLAIPECGHSPHREFPDFVLDAMAKFIDLVLAERK"
     gene            4265..4636
                     /locus_tag="PKZ53_02580"
     CDS             4265..4636
                     /locus_tag="PKZ53_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39349.1"
                     /translation="MENTQPKPIPVQIQLTFNEHGCPVVSIPPSARVEIQVSPGGTDP
                     HDPEIETLKEVIIRGNAEGLTTLAQFLLSVAACQIEGYHQHLDSGAFPSFFRSRDDYW
                     LTIEKTENQKASRNHPSKYVE"
     gene            4626..5093
                     /locus_tag="PKZ53_02585"
     CDS             4626..5093
                     /locus_tag="PKZ53_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39350.1"
                     /translation="MLNNTLTLGSLSFPITKAVFRYVPENNLGGPGWDFNIVAEPCQS
                     LEPDHFLSDRTIRFYSEADPIPVPNLDDLTGVEIFLAEPFDPQSGEVYFTLYVFEHGD
                     LTNLTLRFLERDQSSRYRIFISATVPAGSVFDHDEQLEIHTWIEQLPTVIPEK"
BASE COUNT         1410 a         1273 c         1206 g         1364 t
ORIGIN      
        1 gcttactgtg gcgaaattca cgatgatctc cctgatattg ggagtgaccg ccctgacctt
       61 tgctggtcaa ttcctggaac tgaatggagt caacgggttg atcttactgc tgaaacctgt
      121 gtggttgata aagtccattt cttgattcga ggagtgattg aacttccaat ccacgaggcg
      181 cctgaaactt ttggatttgg agtctgggtt tcccttacca gggaagacct cgatacgtat
      241 ctggaaaatc ctgacactga tgagattggt ccgttttcag gtcggttggg aacaaacatc
      301 cgcttttatg atgaggacac aacctcgctc caaaccctgg ttcatttcct gggtcacaat
      361 gctcgtccaa tcattctgct ggatccaatg acaaatcatc cgctggggat tgatcaacgc
      421 aatggtattt cattgtcgaa agcctgggaa atcgctcatt tttacggagt tgaatgaaac
      481 caaccattct ggcacttgac ctcgaaggca cgctcatttc caatgctgtc agccagattc
      541 cacgacctgg gttgtcagcc tttcttgaac aatgtcacca gcttttttcc agaatcgtga
      601 tgttcacctc agttgatgaa atccggtttc ggcaaattgc caaacgactg gttgaagatg
      661 gggaaactcc tgactggttt gccgagattg aatacatcac ctggaccggg cctatcaaag
      721 atttgacctt cgtcccagat gctacaccag ccaagattgt gctggtagat gactttgaga
      781 gatatattca ccccgaacag aaatcacaat ggattgcgat tgattgcttt caatcaccgt
      841 attcacctga tgacacggcg ttggaagact gcgttcaacg gcttcaagca caggtcaacg
      901 cacatgccgg ttcccaatca gacgattgaa agtgcagaca gaaggaacgt tccaaatgga
      961 agagtatctg gtggaaatca aacagttcca caaacaatcg gttgaaccgt acaccggaaa
     1021 accagttggg tgtatcgaac gggaaatcct gttgcttgaa caaaaaactg gattcgcgct
     1081 cccagaagcc tacaggcagt ttttgcgata tatgggaaaa gacaccgatg gcgtctttcg
     1141 cggctctgga tggtttctgg gtaatatcgg cctcaacgac accctggtac ctgatctttt
     1201 ggaagaaaac gcaatcacat ttcagctccc tgaacattac tttttgtttt tctcgcacca
     1261 gggatatatg gctgcctggt ttgaagtgcc ccggcttaat gacaatccgc cggtgttttt
     1321 cttctccgaa ggcgaggact ggaagggacc tcaaaaaata gacacattta ccacgtttct
     1381 cttcgacgat ttaaaaggat tggcttctct cttaccgcgc ctgtaccgct ttggtgaagg
     1441 agttcaaaaa gatgaagcca gggcacaaaa tcaggccaga caatatccta tccttctcaa
     1501 agctgccttt cgccacttga agaaaaaaca atatgctcag gctctgctgt tctttgatca
     1561 ggccgaagca atcaaaccac tcacggaaaa agaaatggat gcacggcggg tgtgtgaaga
     1621 acgtctggca cgaggttaag accatcttgg gctgagatat cgagctaaga actgaggaaa
     1681 acacaatatt tccaacaagt tagccttccc gtaacacgat atcttcatcg taacctggta
     1741 ttataacctt ttccccttga tgattttaat ccgcacagat tgacccgaag taaattcaat
     1801 tgaatcctgg gtgctcgata tttgtggatc tacatcagat tttccaaggg gagaggaaag
     1861 tggtggattc tgccgattga aagcaattcc ccaaaaggta aaaaatcccg actgattgaa
     1921 aaacctgatc tcctgtgctt gtggtgagta aagataggta tcaccaacac gaaactgatc
     1981 gcgcaaatct atgatatagt tatcgttaat ggatctataa acaactggag aagccaacat
     2041 ttgctgccca taacacacac tgatatcaaa agttctaaaa taccagtgtg gccaggttgc
     2101 ccaaaagaca agcagtcccc caatgaccag aaagattgca agaccagcca gtatcttgaa
     2161 tttctttaat tttaaccagg agggcttttg tttgatccca atggttatga gcatgtttgt
     2221 gcaccttcaa aaccagagtg ttggtgtgag actttcaaag gaaaaatgcc tgaaaacttc
     2281 cttttgaaac cagtcctccg aaatcatttt ccttttcgag ccaggaatgc acaatcaaaa
     2341 tccatcatgt ttcaaggact caaccgagat gcgacttcga aagcgattga ctcaggcgta
     2401 tgttgcggcg ttgctccacc tttcggcgtg gctggccaga cgtgaacggc tcaaccggat
     2461 aacccaatcg tttatgcgct ttctggctgg aattgtcatt cggcgaaaaa acatttcacc
     2521 tcaaacaacg ctttctgagt taggtcaggc gtggcaacag gcgtttccgt ccgtcaaaca
     2581 ccatccgatt accaaaattg acgacacgac cgtttacgga gaaatccaca ccaactgccc
     2641 gctccgtggg acgggagatg tccacgcctg ccatcgcatg atggaatttg accgcacgat
     2701 tgcccgtcag gctggcggag aattcgtcgt gctccggtca caatctgaac ccggtgtcac
     2761 ggtgtgtcag atcgccatgc gattgcgtgg cagtgacacc agtgatttgc agccggctca
     2821 ccaaccaaca cacgctcgtt cctgaaaaac tatgattacc gttggatcag tctcacgcga
     2881 acaagccgcc caaattaggg ttcagggttc agggttcagg gttcaggatt tttcgaattt
     2941 gcacgagact gaagttcaat agtttatgga atggaatttg ctctttcagc accgaaattt
     3001 aatttttgga ggctggctta tgttgcagac ctatcgtgat ttagaagttt ggaaaaaaag
     3061 tattgattta gttgaatcta cttaccaact caccaaatct tttccagcag aagaaaaatt
     3121 tggcctgatt agtcaaattc agcgagccgt cgtttcaatt gctgcaaata ttgctgaagg
     3181 atatggaaga aaacaccgaa aagaatattt gcaccatctt tcaattgcga aaggctcact
     3241 tctggaagta gaaacccatc ttatcattgc aattcgatta cactttacca cccgtgatga
     3301 tgccaaatcg gtttggcatt tatgccaaga tgttggaaga ctgttgaata aacttgtaaa
     3361 ctcactcaat tcaaaaggct taaaccctga accctgaacc ctgaaccctg tattcaaaac
     3421 cctgaacccc gaaccctggc cactcaactg tcatgaattt caaatccagc tttctccgtg
     3481 ttggcaaggt acagcttgaa taccggtggg ttgggccacc gccagacgaa tgtccaaccc
     3541 tggtttttct tcacgaagga ctgggttgtg tgtcattatg gcgggacttt ccagaacaac
     3601 tttttttgca aacagggtgc ggcgcgctgg tgtattcgcg agccggttac ggaaaatcgg
     3661 acccggcttc cctgccccgg ccagtgtggt ttatgcacga cgaagccgaa attctgggtg
     3721 tgattctgga gcaaactgga attaaaaaac cgattttgct aggccacagc gacggcgctt
     3781 ccattgcctt gatttatgcc ggaggaccgc aaggttcctc ggtcagtgga ctgattttgg
     3841 aggcaccgca cgtattcacc gagccgtacg ggttggaaag cattgcgcgg attcgcgaac
     3901 tgtatcgcac cactgatctg cgccagcgtc tggcacgtca tcacggcgac aacaccaaca
     3961 ttgccttttg gggctggaat gacgtctggt tgcatccagc gtttcaagcc tggaatatcg
     4021 aatcagtgtt gccggcaatt acggctccga tgctgattat tcaaggcgag acggatgaat
     4081 atggcacgtg gcgtcaggtc gaagccatcc agcaccagag cggggggaag gttcaaacgc
     4141 tggccattcc ggagtgcggg cactcacctc atcgcgaatt cccagacttt gtactggatg
     4201 cgatggccaa atttatcgac ctggttcttg ccgaaagaaa ataacctctc gattgctgct
     4261 tgttatggaa aacactcaac ccaaaccaat tccggtccag attcagctta ccttcaatga
     4321 acatggctgt ccggtggttt caattccacc ttcagccagg gtggaaatcc aggtttcacc
     4381 aggcggaact gacccacacg atccagaaat tgaaacgctc aaggaagtga tcattcgcgg
     4441 gaatgctgaa ggactgacga ccctggctca gtttttgttg tcagtcgccg cttgccagat
     4501 tgagggttac caccagcatc tggattcagg tgcgttccca tcattttttc gaagtcgtga
     4561 tgattactgg ttaaccatcg aaaaaactga aaaccagaaa gcctctcgca accacccatc
     4621 aaaatatgtt gaataacacc ctgacgcttg gttcgttgtc attccccatt accaaagctg
     4681 tattcaggta tgtcccggaa aataatctgg gaggccctgg ctgggatttc aatattgtgg
     4741 ctgaaccctg tcagtcactt gaacctgacc attttctgtc tgacagaact attcggtttt
     4801 attctgaagc tgatccaatt ccagtcccca atcttgacga tttgactggc gttgaaatct
     4861 ttctcgcaga accatttgac cctcaatctg gtgaagtcta tttcacactc tatgtctttg
     4921 agcacggtga tttgaccaat ctgacacttc gctttctgga acgcgaccaa tcgtcacgct
     4981 atcgcatttt tatttcagca accgttcctg ccgggagcgt ttttgaccac gatgaacagc
     5041 ttgaaattca cacctggatt gagcaactcc cgacggtcat cccggagaag taaaatctga
     5101 catcgagaaa aacctgagga agaattttat gaaaccgatg gttttcaaaa tccgaaccga
     5161 acaccacgcc caacgctgtg aactctgcca ccagaaagat cagtttgatc tcgaaactgg
     5221 ggtttgtgct cgatgtttgg agattcaaaa cac
//
LOCUS       DAOQDO010000101         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10084_length_5251_cov_2.463818, whole genome shotgun sequence.
ACCESSION   DAOQDO010000101 DAOQDO010000000
VERSION     DAOQDO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_5251_cov_2.463818"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PKZ53_02590"
     CDS             complement(<1..1346)
                     /locus_tag="PKZ53_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNJ39351.1"
                     /translation="MLKLLNVLNLALVSRLCIEFEPGLNVLTGETGSGKSIVVDSLGM
                     VLGGRSSPDMIRTGEQKACVEAVFSVPHHPQVLELCAEHGLEVDLDELLIRREITSQG
                     RNRVFIQDQLSTVNFLKQLRPFLVDIHGQGDQQTLMHKEAHSAVLDAFGGIDGLTAQV
                     CTAYEAWDQARTELAAARQGEAERLRRVDMLAFQCQEIEAAKITNTAEDAELEAELHV
                     LSNAEKLVMLATGCFDALYEDESSILTRLASVLRRVDELSAIDPSIEEVLSPLPGVRY
                     VIEDAAFALRDYAERIRFSPERLKQIEDRLTDLTRLKRKYGPTLDDVVRTCLAARTQL
                     KRLGAGEEYARSLEIAVEKAAAVYVELAETLSARRREVAKDFERALMGELKFLAMEAT
                     RIEVALTRMQSDGRPKWGPTGIDEVEFLVSPNIGEELRSLAKVASGGELSRFMLAL"
     gene            complement(1377..2369)
                     /locus_tag="PKZ53_02595"
     CDS             complement(1377..2369)
                     /locus_tag="PKZ53_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ39352.1"
                     /translation="MNHLSLCLRKGEFFSLLGPSGCGKTTTLRILAGLEVPSTGSVWI
                     NGNEITALPPESRGLGYVFQNYALFPHLNVWENVAFGCRLQKLETRQLEQAVCDALEL
                     VRLGEYGKRQVFELSGGQQQRVALARALAIQPQVLLLDEPFSNLDSRLRIETREQVRE
                     IVRKLKVTTVLVTHDREEAFALSDRLAVMEGGEVKQVGTPLEVYFTPQTVEVARFWND
                     GNCVPLTAVPTDVPGLNQTNPTASHVFFRSEDARLAFPGAKPSENPPYPEALAFQGVV
                     HSIQFTGASWLCAVSTPAGQLSIAIAGREQSPETGRPCYVVVPTVNLHFLNGAV"
     gene            complement(2433..4745)
                     /locus_tag="PKZ53_02600"
     CDS             complement(2433..4745)
                     /locus_tag="PKZ53_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39353.1"
                     /translation="MTVHLAGTRTPDTSFVPSSAYPVLQPATRYTPTTFFSPYTRFLN
                     GPRIVWGLFCLVLCGGWGIPTCPVSAATDTQFWEAFRQADILKGTVTNLSVSDRGSLS
                     VSPRNEVIFDTRQPYIWSCVRDGQGGAYIGTGHDGQVYRVDRAGQSRLVADFDELDVT
                     ALAVDSTGKVWAATSPNGKVYQIDPNGKTSVFFNPGEKYIWALERTPRGELVVATGDK
                     GVIYQVDLTGKSVVVFDSTEVNMTSLAIDPRGVIYAGSDPGGIVYRIEGPGKAFAIFD
                     SALREIHRLQIGKNGTVFALAVGDKAVRPASEPLSAGKESVSVTLLADSPSPGAPVNL
                     VNTRSETGGAQSVLYGIEADGGVEIWWGSTELVGLDCVETPQGTVLLGTGPQGRLFEV
                     DRQGQTQLVLQTTEDHISHIMAGASSDLWLTTSNFGKVWKVTPAKALSGVYVSSVQDA
                     KFVADWGMISWRGTGEVRVQTRSGNTETPDGTWSDWSVEATRAEGEPVKSPAARYLQW
                     QIRMTDTSRVDSLRVAYLPKNMAPRVTSLTVLSPGIALQETFQPPLDPAITTAGLDPT
                     LFGLSGTLPPRKVFQKGARSLQWQGEDANGDQLVYQVYYRILGQASWLPLGEAQRASF
                     YLVNADVLPDGLYEFKVEASDMPSNPVSRVLVGYQISDPVEVDNTPPVINVRGEPIHG
                     VTGTTVEFQVIDQMSILRRVEYSVNGSTWSPLSPVDRISDQKMETFRLELKPSTSVQL
                     VAVRAYDGAANAGTVTVMINGSGKESPEAK"
     gene            complement(4762..>5251)
                     /locus_tag="PKZ53_02605"
     CDS             complement(4762..>5251)
                     /locus_tag="PKZ53_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ39354.1"
                     /translation="ALRERREDIPLLVEYFIQRFNARQRKAVRLAEGTMDWLVKQDWP
                     GNVRELEHAVERAVTMNITGELIPQDFLQFGLFDMAQPASSQPAAVQETSSTITSWVC
                     TLPKTLDEVEHEHLLATLSYTQGNKVRAAELLGIGRWSLYRKAKRLGINIGTDNDLSS
                     EK"
BASE COUNT         1257 a         1509 c         1282 g         1203 t
ORIGIN      
        1 ttcagcgcca gcatgaagcg tgagagttcc cctccagaag ccacttttgc caggctgcgc
       61 aattcctctc cgatattggg cgagaccaga aactcaacct cgtcaatccc ggtcggtccc
      121 catttgggcc ggccatcgct ttgcattcgg gtcaatgcga cctcaatccg cgtggcttcc
      181 atagccagaa atttcagttc gcccatgagg gcccgctcaa aatccttggc gacctcccgg
      241 cggcgggcgc tcagggtttc agccagttca acataaacgg cggcggcttt ttcaactgcg
      301 atttcaagcg accgggcata ttcctccccc gccccaagtc gctttaattg tgtcctggct
      361 gcgaggcaag tccgaacgac atcatccagt gttggcccat attttcgttt caggcgggtc
      421 aggtcagtta gccgatcttc aatctgtttt aaccgctctg gggaaaaccg gatccgctcg
      481 gcatagtccc ggagcgcaaa agcggcatct tcaattacat accgtacccc aggcagcggc
      541 gaaaggactt cctcgatgct gggatcaatt gccgataact catcaacccg tcgtaacacc
      601 gacgccagcc gggtcagaat cgacgactca tcttcatata atgcatcaaa gcaacctgtc
      661 gccagcatga ccagtttttc agcgtttgaa aggacatgca gttcggcttc cagttctgca
      721 tcttccgcag tattggtaat tttggccgcc tcgatttcct gacactgaaa ggccagcata
      781 tcgacccgcc gcagtcgctc ggcttctccc tgtcgagccg cagccagttc tgtgcgagcc
      841 tgatcccagg cttcatacgc cgtacaaacc tgagccgtca ggccatcaat accaccaaac
      901 gcgtcgagaa ccgctgaatg ggcttcctta tgcatgagcg tttgctgatc tccctgacca
      961 tgaatatcaa ccagaaaggg tcgcaattgc ttgagaaaat tcacggttga gagttgatcc
     1021 tgaataaata cacgatttcg tccctgactg gtgatctccc gacgaataag gagttcatca
     1081 aggtctactt ccaacccgtg ctcagcacag agctcaagga cctgtggatg atggggaaca
     1141 ctaaacacgg cttcgacaca ggctttctgc tctccggtcc gaatcatatc cggagaggaa
     1201 cgtcccccca ggaccattcc caacgaatca accacaattg attttccgga accagtttct
     1261 ccggttagaa cattcaggcc aggttcaaat tcaatacaca gacgcgagac aagggcgagg
     1321 ttcagaacat tcagaagctt cagcatgccc ggttgatcct cacaaagaaa ataaagtcaa
     1381 acagcaccat tgagaaaatg cagattcacg gttggaacca caacataaca tgggcgcccg
     1441 gtttctggcg attgttctcg accagcaatc gcaattgaga gttgcccggc aggcgtactg
     1501 acggcacaga gccaggaagc accggtaaat tggattgaat gcacgactcc ctgaaaagcc
     1561 agggcttccg ggtacggagg attctcagac ggctttgcgc ctggaaatgc cagtcgggca
     1621 tcctcacttc ggaaaaatac atggctggcg gttgggtttg tttgattcag accaggaaca
     1681 tctgtcggaa cagccgttaa cgggacacaa ttcccatcat tccaaaatcg ggcaacctca
     1741 acggtttgag gcgtaaaata cacttccagg ggggtgccaa cctgtttgac ctccccacct
     1801 tccatcaccg ccagccgatc agataaagca aaggcttctt cccgatcatg ggtgaccagg
     1861 acggttgtta cttttagttt ccgcacaatc tcgcgaacct gctcccgcgt ttcaatacgc
     1921 aaccgggaat caagattact gaacggctca tccagtaaca ggacttgcgg ctggatggcc
     1981 agggcccgtg ccagcgcgac ccgctgctgt tgcccgcctg aaagctcaaa cacctgacgc
     2041 ttgccatact ctcccagcct gaccagttca agcgcgtcac agacggcctg ctcaagctgg
     2101 cgggtttcca gtttttgaag gcgacacccg aaggcaacat tctcccagac gttgaggtgc
     2161 ggaaagaggg cgtaattttg aaaaacatat cccagtcccc gtgattccgg cggaagcgcg
     2221 gtaatctcgt ttccattgat ccacaccgaa ccggtcgagg gcacctcaag gccggccaga
     2281 atcctcaggg ttgttgtttt gccgcagcct gaaggaccaa gcaggctaaa aaactctcct
     2341 ttccgcaggc acagtgacag gtggttcaaa acggtctttc ctgaaaaccg acaggaaagg
     2401 tttcgtaact caacaaagaa atcctgatcc atctacttgg cctccggtga ttcctttccg
     2461 cttccgttga tcatcacggt caccgttcca gcattggcgg ccccgtcata ggcccgaact
     2521 gccaccagtt gaaccgaggt cgagggtttg agttccagtc gaaatgtttc cattttctgg
     2581 tcggaaatcc gatcaaccgg actgagcggg ctccaggtgc tgccattcac gctatactca
     2641 acccgacgaa ggatactcat ctgatcaatc acctgaaatt caacggtcgt cccggtgaca
     2701 ccgtgaatcg gctctccacg tacattgatg acgggtggtg tattgtcaac ttcgacggga
     2761 tcgctgatct ggtatccgac cagtacccgg ctgactggat tcgaaggcat atcactagct
     2821 tccaccttga attcatacag gccatccggc aaaacatcgg cattgaccag atagaaagac
     2881 gcccgctgag cttccccaag cggcaaccag gacgcctgcc ccaggatacg gtagtacacc
     2941 tgatatacaa gttgatctcc gttggcatcc tcaccctgcc actgcagtga ccgggcacct
     3001 ttctgaaaga ccttgcgtgg aggcagggtg ccactcaatc caaaaagggt cggatcaagc
     3061 ccagccgtgg tgatggctgg atcaagtgga ggctggaaag tctcctgaag ggcaattcct
     3121 ggtgataaca ccgtcaagga cgtcactcga ggtgccatat tttttggaag ataggcaacg
     3181 cgcaggctgt cgacgcgact ggtatcagtc atccgaatct gccattgaag gtaacgtgcg
     3241 gctgggcttt tgaccggctc accttcagcc cgggtcgcct caactgacca gtcactccag
     3301 gtaccatcag gtgtttccgt attgccgctt cgtgtttgca cccgaacttc gccagttcca
     3361 cgccaggaga tcattcccca gtcagcgaca aattttgcat cctggaccga cgaaacataa
     3421 actccactta aagctttggc tggtgtgact ttccatactt tcccaaaatt actggttgtg
     3481 agccacaggt cgcttgaagc accagccata atatgtgaga tgtggtcttc tgtcgtctgt
     3541 aagaccagtt gggtttgccc ctggcgatca acttcaaata accgtccctg aggcccggtg
     3601 ccaagcagaa ctgtcccctg cggtgtttca acgcaatcca gccccaccag ttcggttgat
     3661 ccccaccaga tttcaactcc accgtcggct tcgatgccat atagtaccga ttgtgcccca
     3721 ccggtctccg aacgggtatt gaccagattg acaggtgcac caggcgaagg tgaatcagcc
     3781 agcaaggtca ccgaaacgga ctctttcccg gctgagaggg gttcactggc tggccgaacg
     3841 gctttgtcgc caacggcaag ggcaaagacg gttccattct ttccaatttg caaccggtga
     3901 atttcacgca atgcggaatc aaagattgca aaggcctttc caggtccttc aatgcgatag
     3961 acaataccgc ctggatcact tccggcatag atgacccctc gcggatcaat tgccagggaa
     4021 gtcatattga cttctgttga gtcaaacacc accacacttt ttccggtcaa atccacctga
     4081 tagataaccc ctttgtctcc agttgccaca accagctccc cgcgaggggt tctctcaagt
     4141 gcccagatat atttttctcc agggttaaaa aacacacttg tttttccatt cgggtcaatt
     4201 tgatacacct ttccattggg ggaggttgct gcccagacct tacctgtgga atcaaccgcc
     4261 agggcggtta catccagttc atcaaaatcg gcaaccagtc ttgattggcc cgcccgatca
     4321 acccggtaaa cctgtccgtc atgcccggtc ccaatgtagg ctcccccctg tccatcacgc
     4381 acacacgacc agatgtaggg ctggcgggta tcaaagatga cttcattgcg tggtgatact
     4441 gacaggctgc cccgatctga gacagagagg ttcgttacag ttccttttaa aatatcagcc
     4501 tgacggaacg cttcccaaaa ctgggtatcg gtagcagcgc ttaccgggca ggtcggaatt
     4561 ccccaacctc cgcacagcac cagacaaaag agaccccaca ctattcgtgg cccgttcaga
     4621 aaacgggtat atggggaaaa gaatgtggtg ggtgtgtacc tggtggctgg ttgcagtacc
     4681 ggataagcgc ttgaagggac gaacgaagtg tccggtgttc gagtgcccgc aaggtggaca
     4741 gtcattatag ttttgccttt cttatttttc gctggataaa tcattatcag tcccaatatt
     4801 aattccaagt cgtttggctt ttcgatagag tgaccaccgt ccaataccta aaagctcagc
     4861 cgcccggacc ttgtttccct gggtatagct caaagtcgcc agtaaatgtt catgttcaac
     4921 ctcatccagg gttttaggta acgtacaaac ccaactggtg atcgttgacg acgtctcctg
     4981 aaccgctgct ggctggctgc tggctggctg cgccatgtca aagagaccga attgaagaaa
     5041 atcctgtggg atcaattccc cggtgatatt catggtgacc gctcgctcaa cggcatgttc
     5101 aagttcccga acattgccgg gccaatcctg tttgaccagc cagtccatgg ttccttcagc
     5161 cagccggacg gccttccgct gccgggcatt aaatcgctga ataaaatact caaccaggag
     5221 cggaatatct tctcgacgtt cccgtaatgc c
//
LOCUS       DAOQDO010000102         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10156_length_5225_cov_3.210638, whole genome shotgun sequence.
ACCESSION   DAOQDO010000102 DAOQDO010000000
VERSION     DAOQDO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10156_length_5225_cov_3.210638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1001
                     /gene="rplB"
                     /locus_tag="PKZ53_02610"
     CDS             180..1001
                     /gene="rplB"
                     /locus_tag="PKZ53_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNJ39355.1"
                     /translation="MGIKKLKPTSPARRYQTYLTNDELSDVRPLKGLTLARKKISGRN
                     NIGHITVRHRGGGHKKLFRVIDFKRDKSGIVAKVATIEYDPNRSARIALLHYVDGEKR
                     YILAPQGLKIGQQVVSGPESDIIVGNALPLKNIPLGTTIHNIELRPGKGGQMARTAGT
                     SAMLVAKDDKMAQLRLPSGEVRKVSILCQATVGQVGNIENENISFGKAGRSRWIGRRP
                     TVRGVAMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGYKTRKNKNTDKLIVKRRK"
     gene            1098..1385
                     /gene="rpsS"
                     /locus_tag="PKZ53_02615"
     CDS             1098..1385
                     /gene="rpsS"
                     /locus_tag="PKZ53_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNJ39356.1"
                     /translation="MPRSVKKGPFVDKFLTDAVDRMNSAGERRVHKTWSRRSTITPDM
                     VGHTFAVHNGKKFIPVYVSENMVGHKLGEFSPTRTFKGHAGDKNEKTAKRR"
     gene            1494..1871
                     /gene="rplV"
                     /locus_tag="PKZ53_02620"
     CDS             1494..1871
                     /gene="rplV"
                     /locus_tag="PKZ53_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNJ39357.1"
                     /translation="MEARAVAKYIKGSPQKARLVIDQIRGVKANDALALLRLSKKRAA
                     HPISKALWSAISNATYLAEKQNIMVDVDDLVVTRCFVDMGPTKNRRRVRSAPMGRAYR
                     EQRRSCHITVYVSTEKDAQTEKK"
     gene            1881..2537
                     /gene="rpsC"
                     /locus_tag="PKZ53_02625"
     CDS             1881..2537
                     /gene="rpsC"
                     /locus_tag="PKZ53_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNJ39358.1"
                     /translation="MGQKVHPYGFRLGVNRTWRSTWYAKKDFAQLLKEDLEVKKQLKQ
                     KFAGASVSTIDIERAANKLKIIIHTARPGIIIGRKGAEIDKLKAEIQRKTGREVIVNI
                     QEIQKPELSAQLQAEQIAQQLEKRIAFRRAMRKTVENAKKFGAQGVKVRVSGRLNGAE
                     IARSEQYLDGRMPLHTLRADIDYGFAEAHTTYGVIGVKVWIYKGEIYEARRGPARRTA
                     "
     gene            2606..3043
                     /gene="rplP"
                     /locus_tag="PKZ53_02630"
     CDS             2606..3043
                     /gene="rplP"
                     /locus_tag="PKZ53_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNJ39359.1"
                     /translation="MAEYKIPQKVKFRRQQRGRRCGMATRGAAISFGEFGLKALEVAW
                     VSSKQIEAARITITRTVKRGGKLWIRIFPDKPITKKPAETRMGKGKGAPEGWVAVVKP
                     GRILFEMEGVSEELARDAMKLAAQKLPMKVKFVTRADQQGGIV"
     gene            3040..3243
                     /gene="rpmC"
                     /locus_tag="PKZ53_02635"
     CDS             3040..3243
                     /gene="rpmC"
                     /locus_tag="PKZ53_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNJ39360.1"
                     /translation="MKHMTGLRETSTDDLRALETELRETLFRLRFKRSLGDVESARKI
                     ANERKRLARIKTLLRAREIGIEK"
     gene            3287..3622
                     /gene="rpsQ"
                     /locus_tag="PKZ53_02640"
     CDS             3287..3622
                     /gene="rpsQ"
                     /locus_tag="PKZ53_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNJ39361.1"
                     /translation="MAEQDITPQSTTPSDAETPAAEAQATSNPRTPRTEKIGVVTSDK
                     MTKTVVVRVDRLVQHPKYRRYIRKTSKFMAHDELECKIGDKVRILETRPLSARKRWRV
                     VEILQRAAQ"
     gene            3715..4092
                     /gene="rplN"
                     /locus_tag="PKZ53_02645"
     CDS             3715..4092
                     /gene="rplN"
                     /locus_tag="PKZ53_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNJ39362.1"
                     /translation="MIQMRTILDVADNSGAKKISCILPLGGSTGLKATVGDIITANVK
                     EASPDGNVKKGQVVKAVIVRTRKEVRRKDGTYVRFDQNAAVLIKKGSEGWDPIGTRVF
                     GPVARELRDKKFMKIISLAPEVI"
     gene            4094..4426
                     /gene="rplX"
                     /locus_tag="PKZ53_02650"
     CDS             4094..4426
                     /gene="rplX"
                     /locus_tag="PKZ53_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNJ39363.1"
                     /translation="MPAKAKVCKNDLVVVLTGKDVGKRGRVLSVKPRDQKVLVEGVAV
                     AQHHQRNNPQLNRAGGIIEREAYINISNVQVVCPTCGAPTRVGIKVLEDESRKRQCKK
                     CGAIIEQR"
     gene            4514..5068
                     /gene="rplE"
                     /locus_tag="PKZ53_02655"
     CDS             4514..5068
                     /gene="rplE"
                     /locus_tag="PKZ53_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNJ39364.1"
                     /translation="MVPRLKTKYREEITAKMVSQFGYTNPMAVPKLEKIVINMGVGRD
                     YISTKNTKVLEVAADELGSVTGQKSVVTRAKKSIASFKLREKDPIGVMVTLRGDRMYE
                     FLDRLVSIALPRVRDFRGVSGKAFDGRGNYTLGLKDQLLFPEVDFAKVDKARGMNISI
                     VTTAKTDEEGRTLLRFFGMPFRQN"
     gene            5137..>5225
                     /locus_tag="PKZ53_02660"
     CDS             5137..>5225
                     /locus_tag="PKZ53_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNJ39365.1"
                     /translation="MAKKSLIAKANRKPKFDVRGYNRCKRCGRP"
BASE COUNT         1456 a         1249 c         1441 g         1079 t
ORIGIN      
        1 ctgtggtcgc accattggcc gcaagtcgga ttggaaaaaa gcctttgtga cgttaaagcc
       61 aggatccacc attagcgaat atatggaagt gatctaaacc caggggcaat caacctggtc
      121 ggaaccgaaa ccgcaaagtg cattgcagca aattttagaa gaaacctgtg tagggtgtta
      181 tgggcattaa aaagttaaaa ccaacttctc cggcacgacg ctaccaaacc tacctcacca
      241 atgatgagct ttcagatgtg cgcccattaa aagggctcac cctggctcga aaaaagattt
      301 cgggtcgcaa caacattggt cacatcacgg tccggcatcg tgggggtgga cacaagaaac
      361 tgttccgcgt gattgatttt aaacgggaca aaagtggaat tgtcgccaag gtggcaacca
      421 ttgaatatga tccaaaccga tcagccagaa tcgccctgct tcactatgtg gacggcgaaa
      481 agcgctacat tttggcgccc cagggcctca agatcggtca gcaggtggtt tccggtcccg
      541 aatcagacat cattgtcgga aacgccttgc cgctgaaaaa cattccactg ggcaccacga
      601 ttcacaacat tgaattgcgt cctggaaaag gtgggcaaat ggcccgtacc gctggtacca
      661 gcgcgatgct ggtggccaaa gacgacaaga tggcccagct ccggttgcca tcaggtgaag
      721 tccgcaaagt ttcaatcctc tgccaggcga cagtcggcca ggtcggaaac attgagaacg
      781 aaaacatttc tttcggaaaa gctggtcgtt cacgatggat cggtcgtcgt ccaaccgttc
      841 gcggtgtggc gatgaacccg gtggatcacc cacacggtgg cggtgaaggg aaaacctcag
      901 gcggtcgcca tccggttacc ccgtgggggc agccaactcg tggctacaaa acccgcaaga
      961 acaaaaatac cgacaaactc attgtcaaac gtcgcaaata gttacgcttg accacagttg
     1021 gtgtgccacc ctgccatgtc gcagttcctg gcacgtgaag acgaagcacg aaggactcag
     1081 aagcaacgag gaacattatg ccacgatcag tgaaaaaagg gccgtttgtg gacaaattct
     1141 tgacggacgc cgtggaccgg atgaacagtg ccggtgagcg ccgggtgcac aaaacctggt
     1201 cacgtcggtc aaccattaca ccggatatgg tgggtcacac ctttgccgtc cacaatggca
     1261 aaaagttcat tcccgtctat gtttcagaaa acatggttgg ccacaagctg ggtgagtttt
     1321 ctccaactcg taccttcaag ggccacgctg gggataagaa cgaaaagacc gcgaaacgac
     1381 ggtaacgagc cagggcaaaa gtgccgggtg gggtgaaaga tccgtcaaac cgctcacatc
     1441 gtcggtctgg cctctcgaca caaagctctc atcaaacgta gggtggagat tttatggaag
     1501 cacgtgcagt tgcaaagtac attaaagggt cgccgcaaaa ggctcggctc gtgattgacc
     1561 agattcgtgg cgtcaaagcg aacgatgccc tggcattgct ccgattaagc aagaaacggg
     1621 cagcccatcc aatttcgaaa gcgttgtggt cagcaatttc aaatgcgacc tatctcgctg
     1681 aaaagcaaaa cattatggtc gatgtggatg acctggtggt cacccgctgc tttgtcgata
     1741 tggggccaac caagaatcgg cgccgggtgc gttccgctcc gatgggtcga gcttatcgtg
     1801 aacagcgacg gtcgtgtcac atcacggtct atgtttcaac tgaaaaagac gcacaaaccg
     1861 agaaaaagta ggaggcggca gtgggacaga aggttcatcc atatggattt cgattgggcg
     1921 ttaaccggac gtggcggtca acctggtacg ccaaaaaaga ttttgctcaa cttttaaaag
     1981 aagacctcga agtcaaaaaa cagttgaagc agaaatttgc cggtgccagc gtgtccacca
     2041 ttgacattga acgcgcagcc aacaaattga aaattatcat tcacactgcc cgcccaggca
     2101 tcatcattgg tcgcaaaggg gctgagatcg ataaactgaa agccgaaatt cagcgcaaaa
     2161 cgggccggga agtcatcgtc aatattcagg aaattcaaaa accggaactg agcgctcaat
     2221 tgcaggctga acagattgcc cagcagctcg aaaaacgaat tgcttttcgc cgtgcgatgc
     2281 gcaaaaccgt ggaaaatgcc aagaagtttg gcgcccaggg agtaaaagtt cgggtttcgg
     2341 gacgcttaaa tggtgctgaa attgctcgtt ccgagcagta tctggatggc cggatgccac
     2401 tccacacatt gcgtgcagat attgattatg gctttgccga agcccatacc acctatggcg
     2461 tgatcggcgt gaaagtctgg atttacaaag gtgagatcta cgaagcacgg cgcggaccag
     2521 ctcgacgcac tgcctgatca gtgtcggttc ccgtggtttg aacgatccag ggcgaggcta
     2581 attaaaagca taaagtgaga acggtatggc agaatataaa atccctcaaa aggtcaaatt
     2641 ccgtcggcaa cagcgcggac gtcggtgtgg tatggcaacc cgtggggcgg cgatatcctt
     2701 cggcgaattt ggattaaaag ctcttgaagt tgcctgggtt tccagcaagc agattgaggc
     2761 ggcgcgtatc acgatcaccc gtacagtaaa gcgcggtgga aagttatgga tccgcatttt
     2821 cccagacaag ccaatcacga aaaaaccggc tgaaacccgt atgggtaagg gaaaaggcgc
     2881 gcctgaaggg tgggtggctg ttgtcaagcc aggccgcatt ctgtttgaaa tggaaggcgt
     2941 atctgaagaa ctggcgcggg atgccatgaa actggccgcc cagaaacttc cgatgaaggt
     3001 taaatttgta actcgtgctg atcagcaagg agggattgta tgaagcatat gacaggttta
     3061 cgagagacca gcacggatga tttgcgcgct ctggaaacag aattgcggga aaccctgttc
     3121 cggcttcgct ttaaacgcag cctgggggat gttgaatcgg cccgtaaaat cgcaaatgaa
     3181 cgcaagcgcc tggcccggat taaaacgctt ctgcgtgccc gtgaaatcgg tattgaaaag
     3241 tagaacagag tcgagagacc tgaacagatc gaaaaaggaa gtggatatgg cagaacaaga
     3301 catcacgcca cagtcaacaa cgccgagtga cgccgaaaca ccagccgccg aagctcaagc
     3361 aaccagtaat ccacgcacgc cacgcaccga aaagatcggc gtcgtgacca gtgataagat
     3421 gaccaagacg gtggtcgttc gtgttgatcg gctggtgcag caccccaaat atcgacgcta
     3481 cattcgcaaa acttcaaagt ttatggccca tgatgagctc gaatgcaaaa ttggggataa
     3541 agtgcgcatt ttggaaaccc gtccattgtc agcccgcaaa cgctggcgtg tagttgaaat
     3601 tcttcaacgg gcggctcaat aggagcggac agcccgacgt tacctgatga gtgacgtgca
     3661 ggaccaagcc gccggacaag cacaattgcg gcaaagtggg aggaagtcag acctatgatt
     3721 cagatgagaa ccattctcga tgtcgccgat aattcaggcg ccaagaaaat ctcgtgcatt
     3781 ctgcctctgg gcggcagcac cgggttgaag gcaaccgttg gtgacattat cacggcgaac
     3841 gtcaaagaag cgtcgccaga tggaaacgtg aaaaaagggc aggtggtcaa agccgtgatt
     3901 gtccggaccc ggaaggaagt ccgccgtaag gacggaacct atgtgcgatt tgaccagaat
     3961 gctgcggtat tgatcaaaaa agggtcagaa gggtgggatc ccattgggac acgcgttttc
     4021 gggcctgttg cacgtgaact ccgcgacaag aagttcatga agattatctc tttggcgccg
     4081 gaggtgattt gagatgccag cgaaagcaaa ggtttgcaaa aatgacctgg tcgttgtgtt
     4141 gaccgggaaa gatgttggaa aacgtgggcg agtgttgtct gtcaaaccgc gggatcaaaa
     4201 agttttggtg gaaggcgttg ccgtggcgca acaccaccag cgaaacaatc cccagctcaa
     4261 tcgggccggt ggtattatcg agcgcgaagc ctacatcaat atttcaaacg tgcaggtggt
     4321 ttgcccaacg tgtggcgcac caacccgcgt tggaatcaaa gtgcttgaag acgaaagccg
     4381 caaacgccag tgcaaaaaat gcggcgccat tattgaacaa cgctagcgtg atgtagcgag
     4441 cataagcacg cttactgctc cgctcttctg gcgtgaactg ttttcagtct atctaaagtg
     4501 tagagagtta atcatggttc ccagactcaa aacaaaatat cgtgaagaaa ttaccgccaa
     4561 gatggtcagc cagtttggat ataccaaccc aatggcggtg ccgaaactgg aaaagatcgt
     4621 cattaatatg ggggtcggca gagattatat ctctaccaaa aacaccaaag ttctcgaagt
     4681 ggccgctgat gaactgggtt ccgtcacggg acaaaaatca gttgtaaccc gtgccaaaaa
     4741 gtcgattgcc tcatttaaat tgcgcgaaaa agatccaatt ggggtgatgg tgaccctgcg
     4801 tggtgaccgg atgtatgaat tccttgaccg gctggtgagc attgcgctgc ctcgtgttcg
     4861 ggatttccgt ggcgtttccg gcaaggcgtt tgatggacgt ggcaattaca cccttgggtt
     4921 gaaagatcaa ttgctgttcc ctgaagttga tttcgccaag gttgacaaag cgcgtggcat
     4981 gaacatttcg attgtgacga ctgcgaaaac cgatgaggaa ggacgtacct tgctgcggtt
     5041 cttcggaatg cctttccgac aaaactaagc acccgctcag gttgactgcg gagtgaaacc
     5101 caaacttcac gaaaagaagg tgagagtgaa gccgttatgg caaagaaatc cttgattgcg
     5161 aaagcaaacc gtaagccgaa atttgatgtc cgcgggtaca accgttgcaa gcgatgtggt
     5221 cgccc
//
LOCUS       DAOQDO010000103         5168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10344_length_5168_cov_2.693331, whole genome shotgun sequence.
ACCESSION   DAOQDO010000103 DAOQDO010000000
VERSION     DAOQDO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10344_length_5168_cov_2.693331"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKZ53_02665"
     CDS             <1..435
                     /locus_tag="PKZ53_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ39366.1"
                     /translation="GIPGSGKSTWISAFIATTTSPIVIVSTDELRAEFLGDATEQGAN
                     LKIFKIAHSRVRAALRAGKSVMFDATNLLKKHRSQVWRIGRACGARSIAVVLATPLSE
                     ALRRNQTRSRRVPEAVIARFFLQWQRPSLSEADERWVVETEG"
     gene            complement(598..1275)
                     /locus_tag="PKZ53_02670"
     CDS             complement(598..1275)
                     /locus_tag="PKZ53_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ39367.1"
                     /translation="MNRATLLLYCYRCHAPMQPDAQNCDYCGAFRPRSKQENRNEPRK
                     ILLADQFQNRSLSRWTKNLGQWDTRDQKLIGRGGTHYYGLTHSYSFDSRKEYTFRIQG
                     QSRGATGCFGFVSPGLDYGIFLYFHGDGHFDKHMECRFWRGGDTYDFITPRHEVNMSF
                     SGEHRYAFGVRQGSIRVTVDNRYIETIDASQLMTGRYLFSLSNYNPPGTQRVAEHTYS
                     KFQVTVP"
     gene            complement(2028..3989)
                     /locus_tag="PKZ53_02675"
     CDS             complement(2028..3989)
                     /locus_tag="PKZ53_02675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNJ39368.1"
                     /translation="MVSKFERTPYGWPYLLFIWLFGTLSLWLVYQLSMGQGYSVQAVN
                     TQKVLHSAVFLLLLMVCVFLRYRPTRSGALSSEDALNVCVILVFDPGTAIVIMALAAI
                     LNTLSQLANRTVREVEYGPWYANLGEVLFQGGLMAFVTLTGSSVYRVLNSGDWIPFTT
                     LEPRQTASIPFVYASIGLIRWNLMFVHAWTRGTTLLEYNRNLRELRSFVVLLTEISNG
                     LLGVVMAVVYFLDFKAFCALGIVLISLVALLSKQARVTNELQSTVLELKILASLGKAL
                     SNATQTRKELLKALYEHGKELFGADSFAIYLFPEHHTSTDQNHLQLEGVNYRTPRLHR
                     RQTDHSGTGKLRREAIGGETKTPPGGTEISPGKTGELLNSGRWSRQEQTPGEEIPPEE
                     NAIGLAEWCVKRARPLRLEDITKEANAYGYSWLIRQLPYRSWLGVPLELKDRPLGVIS
                     VASESRAAFTEQHQALLQTLGQQVVSALENARLFELATIDGLTGLINPRYLRQKLAEQ
                     FEQAKRTHKPLSVVMIDLDHFKKINDTYGHEVGNDVLRYLAALVKSRLRDSDIPARYG
                     GEEFTVLLPNTPADPSRDVAERLRIAIESAPAPTSAGPIKITASIGVANYPALNVADQ
                     DELVTAADKALYASKQNGRNRITDARFLT"
     gene            4484..>5168
                     /locus_tag="PKZ53_02680"
     CDS             4484..>5168
                     /locus_tag="PKZ53_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNJ39369.1"
                     /translation="MSTLQSSQSDTPQKSKATTTRTTSSGIELNSYYSQADRPEGAPT
                     EIPGSYPYTRGIFAQMYRSKLWTMRQYAGFATAAESNRRYRYLLEQGTTGLSVAFDLP
                     TQIGLDSDNPLALGEVGKVGVAIDSLEDMEVLFDQIPLDRVSTSMTINATAPILLSLY
                     IAVGRKQGVRPDQLNGTVQNDILKEYMARGTYIYPPRPSLRLITDLFAYCSAEVPNWN
                     TISISGYHIR"
BASE COUNT         1347 a         1242 c         1280 g         1299 t
ORIGIN      
        1 ggaattccag ggagcggcaa aagtacctgg atttcagctt ttattgcaac gaccacctca
       61 cccattgtga ttgtttcaac cgatgagttg cgggcggaat tcctcggaga tgcaaccgag
      121 cagggagcca accttaaaat cttcaaaatt gcgcattcgc gggttcgggc tgcgcttcga
      181 gctggaaaat cagtcatgtt tgatgctacc aatcttttga agaagcaccg gtcgcaggtc
      241 tggcgaattg gtcgggcttg tggcgccagg tcaattgccg tggttctcgc cacacccctc
      301 tctgaggcat tgcgtcgcaa tcagacccga agccgtcggg ttcccgaagc cgtcattgcc
      361 cggtttttcc tccaatggca acggccatct ctgtctgaag cggatgagcg atgggtggtt
      421 gaaacggagg gctgaagaag tcgggctgaa gaagtcggtt tcaaggtatt caacccgttt
      481 cttcagctcc gagcttgcga gtcttcagcc cgttttcttc agcccgtttt cttcagcccg
      541 ttttcttcag cccgttttct tcagcccgct ttcttcagcc cgctttcttc agccccatta
      601 aggaactgtt acctgaaact tcgaataggt atgttctgcc acccgctgag ttccaggcgg
      661 attgtaatta ctcaggctaa acaaataccg acctgtcatc aactgactgg catcaattgt
      721 ttcaatatat cggttatcaa ccgtgacccg aatgctgccc tggcggacgc caaaggcata
      781 ccgatgttcc ccagaaaatg acatgttgac ttcatggcgt ggcgtaatga agtcataggt
      841 gtccccaccg cgccaaaacc ggcattccat gtgcttatca aagtgcccat cgccgtgaaa
      901 gtacaggaaa atcccataat ccaaaccggg gctgacaaaa ccaaagcaac ccgttgctcc
      961 gcgtgattga ccctggatcc gaaacgtgta ttccttccgg gaatcaaaac tgtaggagtg
     1021 ggtgagccca taataatggg ttccccctcg cccgatgagt ttttgatccc gggtatccca
     1081 ctgtcccaga tttttggtcc accgcgaaag agaccgattt tgaaattgat cagcaagcaa
     1141 gattttgcgg ggttcatttc ggttttcttg tttcgatctg gggcgaaacg caccgcaata
     1201 atcacagttc tgggcatcag gctgcattgg agcgtgacaa cgatagcaat acagcagcag
     1261 tgttgctcga ttcatgctgg attttctcct gaggattcgg attggcctgc ggcaaagtca
     1321 ggtttttgag caaggtataa acacatcaca atcaagatgc caggaggcat cacgcttcgg
     1381 taactgctta aacctgattt ttctcacaca actgcgttgg aaaacacaaa gggattgagt
     1441 taaccccagt gttattcgaa cgagacttgt tgtcgagttt agatcaaaca agaagttcaa
     1501 cgggtgttgc acagtcacag aatcattgag aatgattgaa tccagacact gagcattgat
     1561 gatggggtca agttgtttcc aggcggaaaa cagaaggcat gtccccagaa tagctcgtct
     1621 gatgcggtga aaactcaaaa aatacacatt gagatagagc ccacaggctg tagccaccga
     1681 tggtggttgg gcattggtca atagattact aaataggccg catgcaaaga ttactgatga
     1741 tggagcttgg gatggcttga gttttgggta atcaaccggc aacaagcagt gggaactttg
     1801 acggttgaag gttcaggttg tttacaggag gatgaaacaa ccctcaaaat gtgcgggcat
     1861 tatacggata tctggaaatg aaagcgagaa aaatctatca aaaaacagga taaaaacaag
     1921 aaaacaagaa aattcttttt ctaggatcag tatttgcagc caatgtgctt atggatgagc
     1981 tttgagcaac gcccatttcc aggaagaatt ttccaaaaca agaaaggtta agtcaggaat
     2041 ctggcatctg tgatccggtt gcgcccgttt tgtttcgagg catagagggc tttatcggcg
     2101 gctgtcacca gttcatcctg atcggcaaca ttcagtgctg gataattggc caccccaata
     2161 ctggctgtga tcttgattgg tccagcggaa gtcggtgctg gggcactttc aatcgcaatc
     2221 cgaagccgtt cggccacatc tctggatgga tcagccggtg tgtttggcaa taaaaccgta
     2281 aattcctcgc ccccatatcg ggcaggaata tcagagtcgc gcaatcggct tttcaccagt
     2341 gctgccagat agcgtaaaac atcatttccg acttcgtgcc cataggtgtc gttgatcttt
     2401 ttgaagtggt cgagatcaat catcaccacg ctcaagggtt tatgcgttcg tttcgcctgt
     2461 tcaaattgtt cagccagttt ttgccgcagg tatcgtgggt tgatcaggcc agtcaatcca
     2521 tcaatggtcg ctaattcaaa caagcgggca ttttcaaggg cactaaccac ttgctggccc
     2581 agggtttgga gcagagcctg gtgttgttcg gtaaatgccg cccgtgactc actggcgaca
     2641 ctgatcacac cgagtggacg atctttcaac tctaatggaa cacccaacca ggagcgatag
     2701 ggaagttgac gaatcagcca gctataacca taggcattgg cttctttcgt gatgtcttcc
     2761 aatcgaagtg gccgggcgcg tttgacgcac cactcagcca gaccaattgc attttcttca
     2821 ggtgggattt cttcacctgg agtttgttcc tgacggctcc agcgaccact gttgagtaat
     2881 tcaccggttt tgccaggtga gatttcggtt cctcccggcg gagttttggt ttcgccgcca
     2941 atggcctccc ggcgtaattt tccagttcct gagtggtcgg tttgccgccg atggaggcga
     3001 ggcgttcgat agtttacccc ctctaattga aggtggttct ggtcggttga ggtgtgatgt
     3061 tcgggaaaca agtaaatcgc aaatgagtct gctccaaaaa gctccttgcc atgttcatag
     3121 agtgctttta agagttcttt tcgagtttgt gtggcattgc tcaaggcttt gcccaaactg
     3181 gccagaatct ttaattcaag cactgttgac tggagttcat tggtcactct ggcctgttta
     3241 ctcagcagtg ccaccagtga gatcaaaacg ataccaagcg cgcaaaaagc tttaaaatca
     3301 aggaaataca ccaccgccat gaccaccccg aggaggccat ttgaaatttc cgttaagagc
     3361 acaacaaacg accggagttc gcgaagattg cgattgtatt caagcaacgt tgttccgcgt
     3421 gtccaggcat ggacaaacat caaattccag cgaatcaaac caattgaggc atagacaaac
     3481 gggatgctgg cggtctggcg gggttcgagt gtggtgaatg gaatccagtc gcccgagttt
     3541 aaaactcgat agacagagct tcctgtgagg gtcacaaagg ccattaaccc tccttgaaat
     3601 aaaacttctc caaggttggc ataccagggg ccatactcga cctcccgcac ggttcgattt
     3661 gccagctggc tgagggtgtt caaaatcgca gccagcgcca tgatgacgat ggctgtgccc
     3721 ggatcaaaaa ccagaatcac acagacattg agggcatcct cagatgacag ggcgccggag
     3781 cgagtcgggc gatagcgtaa aaatacacac accatcaaca acagcagaaa aacagcggag
     3841 tgcagcactt tttgggtgtt tacggcctga acggaatatc cctgtcccat ggataactgg
     3901 tataccagcc acagactcaa agttccaaac agccagataa acaataaata aggccagcca
     3961 taaggggttc gttcaaattt tgacaccata aaagaagaaa gacttgaaaa gaggtaaaat
     4021 ttggctggca acgattcaag cacatgcaag cagaaccgca ggaactgaca gtcgaagtca
     4081 cccgcatcca tgagcctggg gcccggtccc agcaaagagg tgtgtcttta tcaatgtgtg
     4141 aagcaattcg tcttgggttt ggaagtgggt gaaaaaaaca ggaaagctaa aatgtaagcc
     4201 tgttttttct actggacccg gtgtaaattc tgcaagtttt ttttcttgaa aaagcccctt
     4261 ttctactctt ctcgtgaaaa aaagataaag atcttcaagg agatgagatt ttacctggaa
     4321 tctggcctgg aaggtcagtg aatcaatcct ggttcaccaa ttgcatcttt gacacagcgc
     4381 tgagaaaatc agccaaaggt gcgagaattt cccctcctgg ttcaacgttg tttcactcgt
     4441 gacccaaatt cagacttaat tttccaagga tgaagtgacc ggtatgtcaa cattgcaatc
     4501 gtctcaatct gacactccac aaaagagcaa agcgaccacc acccgaacta ccagttcggg
     4561 aattgaactt aattcatatt acagtcaggc tgaccgaccc gaaggggccc caactgaaat
     4621 tcctggatca tatccatata ctcgtgggat ttttgcccaa atgtaccgga gtaagctctg
     4681 gaccatgcgc cagtatgccg gttttgctac ggctgccgag tcgaaccggc gctaccgcta
     4741 tttgctggaa cagggcacaa ccggtctttc agttgctttc gatttgccaa cgcagattgg
     4801 attggactca gataacccgc tggcgttggg cgaggttgga aaggttgggg ttgccattga
     4861 ttcgttagaa gacatggaag ttttatttga tcagattcca ctggatcgtg tttcaacgtc
     4921 aatgaccatc aatgccacgg ccccaatttt gttatcgctt tatattgcgg tcgggagaaa
     4981 gcaaggcgtg cggcccgatc aactcaacgg caccgttcaa aatgacattc tcaaagaata
     5041 tatggcgcgt gggacctata tttaccctcc acgcccatcg ctcaggctga ttaccgattt
     5101 gtttgcctat tgttcggctg aagttcccaa ctggaacacc atttcaatct cgggatatca
     5161 tatccggg
//
LOCUS       DAOQDO010000104         5129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10470_length_5129_cov_3.628104, whole genome shotgun sequence.
ACCESSION   DAOQDO010000104 DAOQDO010000000
VERSION     DAOQDO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10470_length_5129_cov_3.628104"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2729)
                     /locus_tag="PKZ53_02685"
     CDS             complement(<1..2729)
                     /locus_tag="PKZ53_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39370.1"
                     /translation="MRQFVMVILFLFSLLSGGKSLTVPVLGAASNPLSHLTSASSAIQ
                     KPVSVTSRPAGRPAFRVFTDRDGLPENTVRKIVFDQRGYLWVGTLDGAACYNGRVWTL
                     VNMPNRVRSNDISALLAARDGSLWFGTNGGGVSHLKDGQWQTFDETSGLPTNPVFSLA
                     EEIAPDGHSILWLGTYGGGLARFEHNTWTQISSPEIPNDSIVLSILPVTLRDGSHSLW
                     VATRDYGIAVFQNGQWRSYRTTNGFVSDHVEALALMPASTGEPVIWAGTVGGGLARFE
                     GAMFSRVTLPTIVPSNNVQTLAATPLPDGKSCLWVGTDAGVVCLENGNWLRLDDTTGL
                     PGNDIASLAETVSSSGRRTIWIGTNGHGLVRVENGRWARLDLPVENTDHMARCFLETR
                     SATGAPVIWIGTLGSGVLQFANGQWKKLTVTDGLAGNRVRALFETRTADGQSTIWIGT
                     DDGLSKFENGQMVKIDLSQSGIHREVRCLATTTEPDGSPTLWVGTYDSLGRFHNGVWT
                     AYSKRNQTLKNNGVNCLLVTKADNGTQTVWAGTYGGGLSRFENGVWSTCDIASGLPNN
                     IIISLHETKAPDGVRWLWVGTKSGGVARTRLDQSEFKWEVFSDSTQLALPNNCIQRIC
                     EDRQHRLYLFTNKGVARLSPRQATSDDPSLFSVFSFTSEDGLPSNECLEGAALIDQQG
                     RMYAGTIQGTAVYDLETEEADTSARPLFIERIRLNGKWQETTTWLEKVSLAYDENNLM
                     FEYALLNYFRESDTSYRTQLSGFETQPSDWTSDYKKEYTNLSEGQYEFRLWARDYAGN
                     ISGPVKVAFAVRPAPWRTWWAYLMYLAILSTAGYQVYAERIRRIKRRQEERIRYLRHL
                     LDSTRIINSQLDLTTVLQNIAEESAQLIEGEPGGIGLVIGNHLVFKRLWCRDH"
     gene            3105..>5129
                     /locus_tag="PKZ53_02690"
     CDS             3105..>5129
                     /locus_tag="PKZ53_02690"
                     /inference="COORDINATES: protein motif:HMM:NF038117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HNJ39371.1"
                     /translation="MGFLPYVRTCFVFLVMGMVCVVPWSVTQFQMTSQAAARQLTAAP
                     AVVPTPTPGITLAPIGTYRTAKFNVSAAEIVVHDPVSQRLFVVNSDAAAPSIYVLNIS
                     DPTNPTLVTQLTFSSGPVIGDPNSVAVRNGILAVALAATPKTNNGTVAFYNTSTLNFL
                     TSVTAGALPDMVIFTPDATKVLVANEGEPDTVNPPGSVTIIPVSYPSGPGSLSVGSVT
                     TATFTAFNGQEATLRTQGVRIQSGVAAADDFEPEYIAVSSDSTTAWVTLQENNTVAVV
                     NLGTSTVTSLLPLGLKDHNLTGNGLDASDRDLTSSTGKINIQNWPVFGMYMPDSIATM
                     NFNGQTYFLTANEGDARDGEEVRVGSSSYVLDPAVFPNASTLKMNANLGRLTVSNRDG
                     QFEADGDTQYEKIYVYGSRSFSVWTATGTQVFDSGDDFEQITAALFPANFNSNNDANN
                     SFDTRSDNKGPEPEGLTLGVINGRTYAFIGLERISGIMVYDVTNPLSPVFIQYLSTRN
                     FAGDPVADTAGDLGPEGITFISSADSPTGTALLAVGNEISGSTTIFEIRTTEQSVIYV
                     ADTLNNRIQTFDGTQWNVVSGTAALREASEGAGERAAAGSGAGQFRSPEAVTSNNAGG
                     NNLRVYVADTGNNRIHWFDGTQWQDFATFGSGFNQVRAPQGVTLDATGKFL"
BASE COUNT         1286 a         1441 c         1194 g         1208 t
ORIGIN      
        1 cagtgatcgc ggcaccagag tcgcttaaaa accagatgat ttccaatcac cagaccaatc
       61 ccgcccggct caccttcaat caactgggca ctttcctcag caatgttttg caatacggtg
      121 gttaaatcaa gctgcgagtt aatgatccgg gtactgtcga gcaaatggcg gaggtaacgg
      181 atacgttctt cctggcggcg tttgatccgg cgaatgcgtt ccgcatatac ctgatagccg
      241 gcggtactca aaatagccag gtacatcaga taggcccacc aggtacgcca cggtgccgga
      301 cgcacagcaa atgcaacttt caccggaccg ctgatattgc cggcataatc acgtgcccac
      361 agccgaaatt catactgacc ttcagataaa ttggtgtatt ccttcttata atcgctggtc
      421 caatctgacg gttgcgtttc aaagccactc aattgagtgc gatagctggt atcagattca
      481 cgaaaatagt tgagcaaggc atattcaaac atcagattat tttcgtcata ggccagtgaa
      541 actttttcaa gccaggttgt ggtttcctgc catttcccat tcaatctgat gcgttcgata
      601 aataatggcc gggcagaggt gtcggcctcc tccgtctcaa ggtcatacac agccgtgcct
      661 tgaatcgtgc cagcatacat tcgcccctgt tgatcaatca gcgcggcgcc ttcgagacat
      721 tcattactgg gaagcccatc ttcagaagta aatgaaaata ccgaaaacag cgaagggtca
      781 tctgacgtag cctgacgagg agataatcga gcaacccctt tatttgtaaa taaatacagc
      841 cgatgctggc ggtcttcaca aattctttga atgcaattat tgggcaatgc gagttgtgtt
      901 gaatcagaaa aaacttccca tttgaactct gactggtcga gccgggtccg ggcaacaccg
      961 ccgcttttcg tcccgaccca caaccagcgg accccatcag gggctttcgt ttcgtgcaaa
     1021 ctgataatga tgttgtttgg caaacccgaa gcaatgtcac aggttgacca gacaccgttt
     1081 tcaaagcgtg acaacccgcc cccataggtt ccagcccaca cagtttgcgt tccgttatca
     1141 gcttttgtca ccaacaaaca attgacccca ttgtttttca gggtctgatt tcgtttgcta
     1201 tacgcagtcc aaacaccatt gtggaaccgc cccaaactgt cataagtgcc aacccacaac
     1261 gtgggtgacc catcaggttc cgtcgtggtt gccaggcacc gaacttcacg atgaattccc
     1321 gactggctca gatcaatttt gaccatctga ccattttcaa atttactcaa tccatcatca
     1381 gttccaatcc agatggttga ctgcccatca gccgttcggg tctcaaacaa cgcccggact
     1441 cgatttccgg ccagcccatc ggttacggtc agtttcttcc attgcccatt tgcaaactgc
     1501 aacaccccac tgcccaatgt cccaatccaa atcaccgggg cacctgtggc tgaccgagtt
     1561 tctaaaaaac agcgggccat gtggtcagta ttttcaaccg ggaggtcaag tctggcccac
     1621 cgaccgtttt caacccgcac cagcccatgc ccgttggtgc caatccagat cgttcgacga
     1681 cctgaggaag acacggtttc agccaggctg gcaatgtcat ttccgggcag gccggtcgta
     1741 tcgtccagtc gcaaccagtt tccattttcc agacagacca ccccggcatc ggttcccacc
     1801 cacaaacaac tttttccatc tggcaatggc gtcgccgcca gtgtctgcac attgttgctc
     1861 ggcacgatgg ttggcagcgt cacccttgaa aacatagcac cttcaaacct ggccagcccc
     1921 ccaccaaccg ttccagccca tataactggc tcgccagttg aagctggcat cagggccagc
     1981 gcctcaacat ggtcgctcac aaaaccgttc gtggttcgat atgatcgcca ctgcccattt
     2041 tggaagacag caatcccata atcccgtgtg gcaacccaca aactatggct tccatcacgc
     2101 aaagtcaccg gcaaaatact taacacaatc gaatcattgg gaatttcagg cgatgaaatc
     2161 tgggtccagg tattgtgctc aaaacgagcc agtccaccac cataggtgcc gagccagaga
     2221 atggagtgac catctggggc aatttcttct gccaggctaa acaccggatt ggttggcaac
     2281 ccagaagttt catcaaatgt ctgccattgc ccatctttca aatgtgagac accaccgcca
     2341 ttggttccaa accagaggct cccgtctctg gctgccagca gcgcgctgat gtcgttagag
     2401 cggacccgat tgggcatatt gaccagcgtc cagacccgcc cattataaca agcagctcca
     2461 tccagtgttc caacccacaa atacccgcgc tgatcaaaaa caattttgcg caccgtattt
     2521 tctggcaatc catcccggtc agtaaaaacc cggaatgccg ggcgaccagc cggcctggat
     2581 gtgacagaga caggcttctg gatggccgat gatgctgagg tcaggtgaga aaggggattg
     2641 gacgccgccc ccaaaacagg cacggtgagg ctcttcccgc cagacagcaa gctgaagaga
     2701 aataaaatga ccatgacaaa ctgacgcata acgcgcctcc agttgccagt tgacttgcat
     2761 tgttcgggag gaaagtgtgg gacctcaagt ttcaagtaaa caccatgaaa cctggagtta
     2821 caagcacgtt tctagctttc tagcaacctg gcattgcgcg aaacaggaac ctgtcaggac
     2881 cacaggcaac cgaagatgac cccgtttcag cgttgccgtt ctgccatttt gtaacctttt
     2941 gctcacatta cggtaaccca aggctcacag gtctggagta ggaattcacg ttttcatctt
     3001 ttcacgattt tcctcccaac ccctttctca atgcacgcga ccacgtgatt tatgcgaggt
     3061 aactgcgtga caaacaatac ttgagcttta aggagaacca ttcgatgggc tttttgccat
     3121 atgttcgaac ctgttttgtg tttctggtga tgggtatggt atgcgtggtc ccctggtcag
     3181 tgacacagtt tcagatgaca agtcaggcgg cagctcgcca attgacagcg gctccggcag
     3241 tcgtcccaac gccaacacca ggcattactt tggctccaat cggaacgtat cgaactgcca
     3301 aatttaatgt cagcgcggct gaaatcgtgg ttcacgatcc agtttcacaa cgattatttg
     3361 tggtcaatag cgatgcggca gcgccaagca tctatgttct caatatttcc gacccaacca
     3421 atccaaccct tgtcacccag ctcacatttt catccggccc agtgattggt gacccaaaca
     3481 gtgtggccgt gcgaaatggc attctggccg tggcactggc cgccacgcca aaaaccaata
     3541 atggaacagt ggctttttac aacaccagta cactcaattt cttaacctca gtcaccgccg
     3601 gggcactccc ggatatggtg atcttcaccc cagatgcgac gaaagtcctg gtcgccaacg
     3661 aaggtgaacc agacacagtc aatcccccag gctcggtcac aattattccc gtttcctacc
     3721 cgagcggccc agggtcgctg tcggttggat ccgtaacaac cgccaccttt accgcattta
     3781 atggtcagga agccaccttg cgaacccagg gcgtgcggat ccagtccggc gtggctgcgg
     3841 ctgatgattt tgaacctgag tacatcgccg tttcctccga ttcaaccacg gcctgggtta
     3901 ctttgcagga aaataacacc gttgcggttg tgaaccttgg caccagtacc gtcacctcat
     3961 tactcccatt aggtctcaag gatcacaacc tgaccggcaa tggcctggat gcaagtgacc
     4021 gcgatctaac ctcatcaacc gggaagatca atattcagaa ctggcccgtg tttggcatgt
     4081 atatgcccga ttcaattgcc accatgaatt tcaacggcca gacctatttt ctgaccgcca
     4141 acgaaggcga tgcccgcgat ggtgaagagg tacgagttgg atcttcgtcc tatgtccttg
     4201 atccagcagt gtttcctaat gcttcaaccc tgaaaatgaa cgccaattta ggacggctta
     4261 ctgtttcaaa ccgggatggt caattcgaag ccgatggcga tacacaatat gaaaagattt
     4321 atgtctatgg cagccggtca ttttcagtct ggaccgccac cgggactcag gttttcgata
     4381 gcggcgatga ttttgagcaa atcacagccg cactcttccc agcaaatttc aattcaaaca
     4441 atgacgccaa caacagcttt gatacccgaa gcgacaacaa aggccccgag ccagaaggat
     4501 taacacttgg ggttatcaat ggtcgtacct atgcctttat cgggttggaa cgcatcagcg
     4561 gcattatggt ttatgatgtc accaatccgt tgagcccggt gtttatccaa tatctgtcaa
     4621 cccgcaattt tgctggcgac ccggttgctg atacggcagg cgacctggga ccagaaggaa
     4681 tcacctttat ttcttcggct gatagcccaa ccggaacggc attgctggcc gtgggcaacg
     4741 aaattagtgg gagcacgact atttttgaaa ttcgaaccac tgagcaatct gtcatttacg
     4801 tggcggatac gctcaataat cggattcaaa cctttgatgg gacccaatgg aatgttgttt
     4861 ctggaacagc cgctctgcgc gaagcatcag aaggagccgg tgaacgagct gccgctggtt
     4921 ccggcgccgg acaattccgc tcaccagaag ccgttacctc caacaatgcc ggaggcaaca
     4981 acttgcgtgt ctatgtcgct gatactggaa acaatcgcat ccactggttt gatggaacac
     5041 agtggcagga ttttgccacc tttggttctg ggttcaatca ggtgcgtgcc ccacaggggg
     5101 taacccttga tgcaactgga aaattcctg
//
LOCUS       DAOQDO010000105         5128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10473_length_5128_cov_2.820816, whole genome shotgun sequence.
ACCESSION   DAOQDO010000105 DAOQDO010000000
VERSION     DAOQDO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10473_length_5128_cov_2.820816"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKZ53_02695"
     CDS             <1..272
                     /locus_tag="PKZ53_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39372.1"
                     /translation="SFSDKQSSDARLALAKARASAAKASEFMAKTIVVCESCMTAGDK
                     ESATYGKLLTSCRTDIEDPFRVSHNNKLDGLDEYIQSMRTRICSK"
     gene            431..1174
                     /gene="rnc"
                     /locus_tag="PKZ53_02700"
     CDS             431..1174
                     /gene="rnc"
                     /locus_tag="PKZ53_02700"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNJ39373.1"
                     /translation="MNLSELEERIGYQFQRPELLERALTHRSYANEHSHENCLSNEGL
                     EFLGDAVLGLIISEWLVERFPDSPEGVLARFKGYLVSSLHLAECAQMLELGKYLRLNR
                     GEEKTGGRRKRAILENSFEAILAALYLDAGLARTATFVRTLFEPSVATLDPTEANFAD
                     SKTALQDWLRSHRMPLPEYFTVASDGPPHSPEFSVGLRLGSEVIAQGRGPTLKAAHQA
                     AASMALKYLKTRHLATRTLKAQDPPPSIP"
     gene            1333..2232
                     /gene="lepB"
                     /locus_tag="PKZ53_02705"
     CDS             1333..2232
                     /gene="lepB"
                     /locus_tag="PKZ53_02705"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNJ39374.1"
                     /translation="MFKSAFARFQTAINLNQNSGLDDVTNASSQSSLIQRKRGVVREC
                     IEAGLMTVIGSLFIFTFVVQDVRVETGSMKNTIQVGDYLLINKFMFADGGSTGIPWLP
                     TRTIHRGDIVIFKFPKDPNVNYVKRVVGLPGETIEIRGNRVFINGRELAEHRFTINIE
                     SDRLNRTAPLQILAELPVLDLGPDTVTSTAHYKVYYGPQPNDPNDPGTRQVVGELLDT
                     AVYGVGSPYKIPPDHFFCLGDNRDNSEDSRQWGGVPRKNIIGAPMWILYSIRAEDHHS
                     GSANFLIDVLSNSKWDRFGTRIR"
     gene            2247..3950
                     /locus_tag="PKZ53_02710"
     CDS             2247..3950
                     /locus_tag="PKZ53_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNJ39375.1"
                     /translation="MSTVESPQIPPTIKIAKTHLPPHRPRSSRLLWKIGGVIAVFLVL
                     AVLVVLFPSVIPTGATKSRLEQKLGILTGRNTRIGTVSIKSIVFPTLVLEQVELDDDP
                     AFQKGSFLRVRRLETRVPFSGLWGASLEVSQLKVEGAQLNLVHRLDGEWNWSSLRLLK
                     PNPLAAIRHQATLPENWFDLPSVQASHQSHPLALALLELPLEIEKIQVFDSSFVVSSE
                     SKKEAAEKDEPASQANSATPSDTKPGIEVNIFRCSNISTEGVFSKNRLACRSLMASLA
                     NGTYQGELAIDLLPQMFQFEIKGSLKGAEMKEILQALSTKPVVTGAVSGDFRLRGKFA
                     EKTDVSRSLEGEGKLELRDGTFPTIRIGEFSKKDTPQLPIPSGFPFPIPKLPFPQQLP
                     TPGPEEPQSSEYFMFAGGKPGAKKPAAEWKGGVAPGATESQKSEGTRFELLTCSFSVD
                     PEKFQAKELMCNTKEKTEVAGHGTIFFRGSVTTVDFQLKATMTAEMAAKLQKSRPVAS
                     VISSLLSQGKTIRLPLKVTGDIRKPDVEVDYVGFVRPGESQRDGDANQLPIPDSLSLP
                     F"
     gene            complement(4120..4395)
                     /locus_tag="PKZ53_02715"
                     /pseudo
     CDS             complement(4120..4395)
                     /locus_tag="PKZ53_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            4552..>5128
                     /locus_tag="PKZ53_02720"
     CDS             4552..>5128
                     /locus_tag="PKZ53_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39376.1"
                     /translation="MTAPIKIVTECELSNAEFLERYAREGCVGLAGGPSLVDKAIRRA
                     QRRQRPDRSWSEWGHAFVFQGRRPDGHHWVIESDLEFHRRNIRLGVQENRITKYHDEE
                     YFQSLAILDFHLEPVQVSTLIAAGLDLVATNTKYSLREILGTYLALHNIGERATANRM
                     QKERSLFCSAFVRHLYSKVGLDFAPQIHEKHT"
BASE COUNT         1287 a         1228 c         1297 g         1316 t
ORIGIN      
        1 attcgttctc cgataaacaa tcttcagatg cacgcctggc actggccaag gcccgtgcca
       61 gcgcagcaaa agcatcggaa tttatggcaa aaacaatcgt tgtttgtgaa agctgcatga
      121 cggcaggcga taaagagtca gccacctatg gtaaactact gacctcttgc cgaacagaca
      181 ttgaggatcc gtttcgtgtc agccacaata ataagcttga tgggctggat gaatatattc
      241 aatcgatgcg aactcgaatc tgttcaaaat agcagaagct ggtttgagga aatgatggac
      301 tctcccccac gggaatgttg acggctggtc atcagatgtt gagatgtcca gttcggcgat
      361 ggctgccagg gattcatttt ttctggtcaa aggatgtact aattatcaaa agcggaaagg
      421 gcaatagatc ttgaatctca gtgaacttga agaacgcatt gggtatcagt tccagaggcc
      481 tgagttgctt gagcgagcct tgacacaccg ctcgtatgcc aatgagcata gtcacgaaaa
      541 ctgtttaagc aatgagggcc tcgaattctt aggcgatgct gtcctggggc tgattatcag
      601 tgagtggctg gttgaacggt ttccagactc tcccgaaggt gttctggccc ggtttaaggg
      661 gtatctggtc agttctttac atctggctga gtgcgctcaa atgctggaac tgggaaagta
      721 cttgcggttg aatcgcggag aagaaaaaac tggtggccgc cgaaaacggg cgattcttga
      781 aaattcattt gaagcaatcc tggccgcact ctaccttgat gctggcctgg cccgaacagc
      841 gacttttgtg cgaacacttt ttgagccttc agtggccacc ttagacccaa ctgaggccaa
      901 ttttgctgat tcaaaaacgg cattacaaga ctggttgcga tctcatcgaa tgccgttacc
      961 agagtatttt accgttgcct cagacggccc gccccacagt cctgaattct cagttgggtt
     1021 gcggttaggc agcgaggtga ttgctcaggg gcgtggccca acgctcaaag cggcccatca
     1081 ggctgcggca agtatggcac tgaagtatct caaaactcgt catttagcga cccgcacttt
     1141 aaaagcccag gaccctccgc cgtcaatacc ttgatcaggc cgtcaaagca gcacagattg
     1201 cattcattgc acttgatttg atttctttac cggctggttt ttggtacctt cgacggcaaa
     1261 tctacctgta ttctgtcaga aagagcagtc ccaggaggac tgattcatcc tggtcatcat
     1321 ttcttaaatt ccgtgttcaa atctgccttt gcccgctttc aaactgcgat caacctgaac
     1381 cagaactctg gtttggatga tgtaaccaat gcctcatcgc aatcgtcgct cattcaacgg
     1441 aaacgagggg tggtgcgtga atgtattgag gctggtttaa tgacggtgat tggttcgctt
     1501 tttattttta cctttgtcgt ccaggatgtc agggttgaaa ccgggtcaat gaaaaataca
     1561 attcaggtgg gtgattacct gttgatcaat aagtttatgt ttgcagatgg cgggtcaacg
     1621 ggtatcccct ggctgccgac ccgcaccatt catcgtggtg atatcgtgat tttcaagttt
     1681 ccaaaagatc caaatgtcaa ctatgtcaaa cgtgtggttg gattgcctgg agagacgatt
     1741 gaaattcgcg ggaaccgcgt ctttatcaat ggccgtgaac tggctgaaca ccggtttact
     1801 atcaatattg aatcagaccg gttaaatcga acggcgccgt tgcagatcct ggctgaactt
     1861 ccagttcttg atctgggacc tgacactgtg acctcaactg ctcactataa ggtctattat
     1921 ggtcctcagc caaatgaccc aaatgaccca ggaacccgac aggtcgtggg tgagttgctt
     1981 gatacggctg tctatggggt tggaagccct tataaaatcc cacctgatca ttttttttgc
     2041 cttggggata accgcgacaa tagcgaagac agccggcaat ggggtggtgt acctcgaaaa
     2101 aatatcattg gcgccccgat gtggattttg tattcgattc gggctgaaga tcatcactct
     2161 ggatccgcta attttttgat cgatgtgttg tcaaattcaa agtgggatcg ctttgggact
     2221 cggattcgat aaaaggggaa tttggtatgt caaccgttga atcgccgcag atacctccga
     2281 caattaaaat tgcgaaaacc caccttcctc cccatcggcc cagatcgagt cggttgctgt
     2341 ggaaaatcgg cggagtcatt gccgtgttcc tcgtgctggc cgtgctggtg gttttatttc
     2401 cttctgtgat ccccaccggc gccacaaaat ctcgacttga acaaaaattg gggattttaa
     2461 ccgggcgcaa tacccggatt gggacagttt caatcaagtc tattgtgttt ccgacactgg
     2521 ttttagaaca agttgaactg gatgatgacc cggcatttca aaaagggtct ttcttacggg
     2581 tccgccgcct ggaaaccaga gtcccgttca gtgggctctg gggcgccagc cttgaggtgt
     2641 cacagcttaa ggttgaaggg gcccaactca acctggttca tcgccttgat ggggaatgga
     2701 actggtcttc tctccgattg ctcaagccca atccactggc tgccatccgc caccaggcca
     2761 ctctgcccga aaactggttt gacctgccat cggttcaggc aagccatcaa tcccatcctc
     2821 tcgcgctcgc cttgctcgaa ctacctttgg aaatcgagaa aattcaggta tttgattcgt
     2881 cgtttgttgt cagttctgaa agcaaaaagg aagctgcgga aaaagacgaa cccgcttctc
     2941 aagcgaattc agcgactcct tctgatacca agccaggtat tgaagtcaat attttccggt
     3001 gttccaatat cagtaccgag ggggtttttt ccaaaaatcg actggcctgt cggtcgctga
     3061 tggcatctct ggcaaacgga acgtatcagg gcgaactggc cattgatttg ctcccccaga
     3121 tgtttcaatt tgaaattaaa ggctcgctca agggggcgga aatgaaagag atcctgcagg
     3181 ctctcagcac caaaccagtg gtcacgggtg ctgtcagcgg tgactttcgc ctgcggggaa
     3241 aatttgcaga aaagacagat gtcagccggt cacttgaagg agaagggaag ctggaattaa
     3301 gggacggcac ctttcccacg attcggattg gggagttttc aaaaaaagat accccgcaac
     3361 tcccgattcc ttctggtttt ccgtttccaa tccccaaact gccatttcca caacaactcc
     3421 ccacgccagg acctgaggag ccccaatcat cagaatactt tatgtttgca ggagggaaac
     3481 caggcgccaa gaaaccagct gcagagtgga aaggaggggt tgcaccaggg gcaaccgaat
     3541 ctcaaaaatc cgagggaaca cgctttgaac tgttgacttg cagcttctca gttgatccag
     3601 aaaagtttca ggccaaagaa ttgatgtgta acacgaaaga aaaaacagaa gtcgccggtc
     3661 acggtaccat tttctttcgt ggaagtgtga ccacggtgga ttttcaactc aaggcaacca
     3721 tgacggctga aatggctgcc aaattgcaga aatcacgacc agttgcctct gtgatttcaa
     3781 gtttgctcag tcaggggaaa acaattcggc tcccactcaa ggtcaccgga gatatccgca
     3841 agccggatgt tgaagttgac tatgtgggat ttgttcgacc aggagaatcc cagagagacg
     3901 gagacgccaa tcaattgcca attcctgatt cgctctcgtt gccgttttag ggatttgggg
     3961 ctgaagaaga cgggctaggg gctaggggct gaagaagacg ggctaggggc taggggctga
     4021 agacatcggg ctgaggaatt ggtttttttc accccctcat ccttcatccc tcaccccttc
     4081 aattgctccg agcttgcgag tcttcagccc aaggatcttt cagcccgatg tcttcagcat
     4141 ttgaagaaat cgctggagca gcccgcctcc tgacgttgat tgagaaattt ctgattgggc
     4201 ctgttccagg ggggttccct tttcaagtgc cgcaatcagg tcggtcactt cgtttgggtt
     4261 tggaaaacgt ccaagacgtc gtttggaaaa gatcaattca tcatcgcggt agacttcaaa
     4321 aattccgcct gaaagtttga ccagggttgc cgtaacacca aattttgctt taagttcttc
     4381 tgccaacctg acagctttgg gatagtaccc tcaagatcca caatattcga ctttgagctt
     4441 catggaaaag tacctcgaag tgtaaaacgg ttgttgatgg ttttttggga aaagggttga
     4501 aaaacattca aactatagca cttcttttag atataaggag ttgaaattac cgtgactgca
     4561 ccaatcaaga ttgtgacgga gtgtgaattg tccaatgctg agtttctgga gcgctatgcc
     4621 cgtgaaggat gcgttgggtt ggctggcggg ccatcgctgg ttgataaagc cattcgccgg
     4681 gcacaacgcc gacaacgtcc agaccgaagc tggagcgaat gggggcacgc tttcgttttt
     4741 caaggccggc gaccagatgg ccaccattgg gtgatcgaat cagacctgga atttcaccgg
     4801 cgaaatatcc gtttgggtgt tcaggaaaat cgcatcacca aataccacga tgaggagtac
     4861 ttccaatctc tggccattct cgattttcat ttggaaccag ttcaggtcag caccttaatt
     4921 gctgctggat tggatctggt ggcaaccaat accaaatatt cactgcggga aatcctgggc
     4981 acctacctgg ccttgcacaa tattggtgaa cgtgccaccg ccaatcgcat gcaaaaagag
     5041 cggtctctct tttgctcagc ttttgtccga cacttatatt caaaagtcgg ccttgacttt
     5101 gctcctcaga ttcacgaaaa gcacacca
//
LOCUS       DAOQDO010000106         5101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10561_length_5101_cov_3.005153, whole genome shotgun sequence.
ACCESSION   DAOQDO010000106 DAOQDO010000000
VERSION     DAOQDO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10561_length_5101_cov_3.005153"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKZ53_02725"
     CDS             <1..288
                     /locus_tag="PKZ53_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39377.1"
                     /translation="GLPLWAHHRISVRSSRPDGRCTPGSISCFPDLDPSADHFDPAPI
                     RARTSMAALSGGRMSKATFATGYFPPPIRAKTNLVPNQMIEFHSLFMGTCT"
     gene            complement(333..827)
                     /locus_tag="PKZ53_02730"
     CDS             complement(333..827)
                     /locus_tag="PKZ53_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39378.1"
                     /translation="MTLTDLQADIYQALGERAIPVIYREKILTERTRQFVLFPPDTPH
                     VTPEKVHIHYTLLGIELKLGTRRLSCPDLGTARFLQVFGRLGVGIVAVPYDISQIPRI
                     ADDLESGWQHLLLLIEDRAGDRPLRTRLTIRNRLMEQMRQDIEKLGPGTKVPQFNQNT
                     RQRR"
     gene            complement(1026..2012)
                     /locus_tag="PKZ53_02735"
     CDS             complement(1026..2012)
                     /locus_tag="PKZ53_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNJ39379.1"
                     /translation="MANGVLVFIEQRDGKIRKASYEALSEGRRLADKLGQSVSALVIG
                     SGINELATEAAHYGADTVYVTDNAALKNYSTDGYAKVLAEVIQTAQPAVVLASATALC
                     KDLAPRVTARLNIGLASDITEIHVEGGAVSVVRPVYAGKAFAKVAFRSATAFVTLRPN
                     VFPASAPDASRTAEVKAVGGVMVDGLQAKVTDILAAAGGKVELTEANIVVSGGRGIKG
                     PENYYLVENLADALGGAAGASRAVVDAGWVDHSRQVGQTGKTVSPQLYVACGISGAIQ
                     HLAGMSSSKFIVAINKDPDAPIFKLADYGIVGDLFEVLPRLTEQAKALRAKK"
     gene            complement(2034..2819)
                     /locus_tag="PKZ53_02740"
     CDS             complement(2034..2819)
                     /locus_tag="PKZ53_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNJ39380.1"
                     /translation="MKIAVCLKAVPDTDTRIKIAADGRSIDRSEIKYIMSPYDEYALE
                     EAIRTKEAKGGDVVVVGLGGDEVPAVLRDGLARGGDSAIHVSSNGVATEDPLAVGKAL
                     AAALKSVNADIIFFGKHGVGGDNQQVHTIVAELLGLPQATMVVKLQINDRNVRCEREI
                     EGGLEVIETTLPVVIGANKGMNEPRYPKLPAIMQAKKKPFATKTIADLGLSAADVSEA
                     ASATYITGMYLPPARQAGRKLEGDVNEQVTGLIQALQGEAKVL"
     gene            complement(3329..>5101)
                     /locus_tag="PKZ53_02745"
     CDS             complement(3329..>5101)
                     /locus_tag="PKZ53_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ39381.1"
                     /translation="LYAVHLWDAHTGQEIRRLAVSSEGIHHARFSSEGGRIATLQSGK
                     LQLWDCATGKELHLPGLTTRTDVQEFSFSTRHNHLLTVHQSGLVTTWDLETGTPVTQR
                     RTTKTDEILCNTFSPEGKTLAQGQTNGQILVWETGANTPVKKLTGHQDRVTALTFSQD
                     GKRLFSTGNDGFIRTWNLETGIELNRVKKHSTWDNELAISPDETLLLLTVWSNSICQL
                     QDAKTGRELCRLQGSAIPIYAAAFSVDGNTLVLAGDQNDMRPWHGPCQHCTVSVWNLQ
                     TGQEQTRWSPRLPGVRSLAIFPDGQGIAIGGASGLQVSRLHTGQPDWANEFYADRLAL
                     SPNGQWLCASGHAGTVLFQATTGQKERELIRHRAFAIAFAPNGEQVATSHYQSVEVWD
                     VATGQLLRRWEFPNQWIYGLAYTKDNQSLLIGNLDGELRRCDIATGKELWKVQDSSEG
                     ISQIVLSPDGKIAAIGREEWRVQFWEIEQGQVLSELQRNPEYISDIAFHPSGKIILTA
                     SQEASVRLWELATRNQLCLLAIQNDGNWMVVDQHNRFDLFAPQSMTSAHWVENKKPVF
                     AHERYHSGYTPGLLAHLAQTSRGK"
BASE COUNT         1139 a         1354 c         1361 g         1247 t
ORIGIN      
        1 ggtttgccgc tttgggcaca ccaccggatt tcggtccgct cttcgcgccc ggatgggcgc
       61 tgcacgcctg gttcgatctc ttgttttcct gaccttgacc catccgctga tcattttgat
      121 ccagcgccca tccgggcgcg aacctcgatg gccgctcttt ccggtggccg catgtccaaa
      181 gcgacatttg ccaccggcta ttttccaccg cccatccggg cgaaaaccaa tttggttccc
      241 aatcaaatga tcgaattcca ttccttattt atggggactt gcacttagcg atctactggc
      301 tactgaccac taactacaaa ctggtatgaa tattatcgtc gctggcgggt attttgattg
      361 aattgaggga ctttggtgcc aggacccagt ttttcgatgt cctgacgcat ttgttccatg
      421 agccggttgc ggattgtgag gcgtgtccgc aagggccggt ctccggcacg atcttcaatc
      481 aacagcaaca ggtgttgcca gccggattca aggtcatcgg caatgcgggg aatctgactg
      541 atgtcgtatg gaacagccac gataccgaca ccaagtcgtc caaacacctg caaaaagcgg
      601 gcggttccca aatcagggca cgagaggcga cgggtgccaa gtttgagttc aatccccagc
      661 aaggtgtagt gaatatggac tttttcggga gtaacgtgtg gagtgtcagg cgggaaaagt
      721 acaaattgcc gggttcgctc agtcaaaatc ttctcgcgat aaatgactgg gatggcgcgc
      781 tctccaagag cttgataaat gtcggcctgg agatcagtaa gtgtcatgaa aatggaggga
      841 agaattgaga atgaagaatt gagaatgaag aattgagaat gaagcatgaa gttcgtggca
      901 agtgattggt ccgtggtttc tgacttttct tgggaagtag tgatttctaa ccactaacca
      961 ctaaccacga gaaaggttct tcattcttca ttcttcattc ttcattcttc attccctggt
     1021 ctggcttact tcttggcgcg gagcgctttg gcctgttcgg tcaagcgtgg gagaacttca
     1081 aacagatcgc ccacaatacc gtaatcagcc agtttgaaaa tcggcgcatc cgggtctttg
     1141 ttgatcgcca cgataaactt ggacgatgac atacccgcca gatgctggat ggcgcctgaa
     1201 attccgcagg ccacatacag ttgcgggctg acggtttttc cggtctgacc gacttgacgt
     1261 gagtgatcaa cccatccggc atccaccacg gcgcgtgagg cacccgccgc cccacccagg
     1321 gcatctgcca ggttttcgac cagataataa ttttccggtc ctttgatccc gcgaccgcct
     1381 gagaccacaa tgttggcttc ggtcaattcg accttgccac ctgcggcagc aagaatgtcg
     1441 gtaaccttgg cttgtagtcc atccaccatt acgccaccaa cggctttgac ttcagccgtt
     1501 cggctggcat ctggggcact ggctgggaac acatttggcc gcaacgtcac aaaagccgtg
     1561 gcgctccgga acgcgacttt ggcaaaagct ttcccggcat agaccgggcg aaccactgac
     1621 accgcgccgc cttcgacgtg gatttcagtg atgtccgaag ccagtccgat attcaaccgg
     1681 gccgtcaccc gtggcgcgag gtctttacac agcgcggtgg ctgacgccag cacgacggct
     1741 ggttgcgccg tttggatcac ttcggccagg actttggcat agccatcggt cgagtagttt
     1801 ttcagggcgg cattgtcggt cacatagacc gtatccgcgc catagtgagc ggcttcggtg
     1861 gccagctcgt tgattccaga accaatgacc agtgcgctca ctgactggcc cagtttgtct
     1921 gccaggcggc ggccttctga aagggcctca tagctggctt tgcgaatctt tccatcgcgt
     1981 tgctcaataa acactaacac tccgtttgcc atatcaattc ctttcctttt tctttagagg
     2041 accttcgctt cgccttgcag agcttgaatc aggccagtga cctgttcgtt gacatcacct
     2101 tcgagcttgc gcccagcctg acgggccgga ggcaaataca taccggtaat gtaggtggct
     2161 gaagctgctt cgctgacatc agccgctgag agccccagat ccgcgatggt cttggtggcg
     2221 aacggttttt tcttggcttg catgatggcc ggcagctttg gatagcgcgg ttcattcatg
     2281 cctttattgg cgccgatcac aacgggcagg gttgtttcaa tgacttccag accgccttcg
     2341 atttcacgtt cacagcgcac gttgcgatca ttgatctgca gtttgacgac catggtcgcc
     2401 tgtggcaggc ccagtaattc agccacgatg gtgtgaacct gctggttgtc gccgccaacc
     2461 ccgtgtttgc caaagaagat aatgtcggcg ttgactgact tgagggctgc cgccagggct
     2521 ttgccaaccg caagtggatc ttcggtggcc acgccgttgc tgctgacatg gatggctgaa
     2581 tcgccgccgc gagccagacc gtcacgcaga accgccggca cttcatcccc accaaggcca
     2641 accacgacca catcaccgcc tttggcttct ttggtccgaa ttgcctcttc gagggcatat
     2701 tcgtcatatg ggctcataat gtatttgatt tcggatcggt cgatggaccg accatctgcc
     2761 gcgattttaa tgcgggtgtc ggtatccggc accgctttga gacatactgc aattttcaag
     2821 gtgacctccg agactataaa gagaagatta agactgaaaa cacaatcact gattgaccca
     2881 gatcgcctac tcgtcaggat gaaggggcaa acataattga gcggaatgaa ggatgaaaaa
     2941 tgaagaaaat gataacgtgt tgcagatcaa caggataacc ttttcataat tcataattca
     3001 tacttcatct gagggctggg aaatcaggga attttgagtg atggctgtct gattcccctg
     3061 gagttggcac tgatggaccg catcgaaacc gctttgggga ggatccaggg ctcgaaaccg
     3121 gatatcaatc aagagaacgc agacagttga aatcacgaga agctgtgcaa atggaaggtt
     3181 tatcacgttg tgcgattggc tgcaatacag agaagtggag aaatgacgga ttgcgtcaaa
     3241 atgcagcaaa aaacctttgt tttcgaccct gggtggcttc agtttgagcc gtcagcgccc
     3301 ttgaaagaac tgaatcctgg cttcgccgtc actttcctcg acttgtctga gcgagatgag
     3361 ccagcaggcc cggcgtgtat cctgagtgat agcgttcgtg agcaaagacc ggttttttat
     3421 tttccaccca atgggccgac gtcattgact ggggcgcaaa cagatcaaac cggttatgtt
     3481 gatccaccac catccagttc ccatcattct gaatggctaa aagacaaagc tggtttcgcg
     3541 tggccagttc ccacagccgc accgaagcct cctggctggc cgtgaggatg atttttcctg
     3601 atggatgaaa ggcgatgtcg ctgatgtact ctggatttct ctggagttca ctcaagacct
     3661 gaccctgttc aatttcccaa aactgaacgc gccattcttc tctaccaatc gctgcaattt
     3721 ttccgtctgg cgaaagaacg atttgggaaa ttccctctga tgagtcctgg actttccaga
     3781 gttcctttcc ggtcgcaata tcacagcgcc gtagttcacc atccaaattc ccaatcaaga
     3841 gcgactggtt atctttggta taggccaatc catagatcca ctggttgggg aattcccacc
     3901 ggcgaagcag ttgaccggtg gcaacgtccc agacttcaac cgactggtaa tggctggtgg
     3961 cgacctgttc accatttggc gcaaaggcaa tggcaaacgc ccgatgtcgt atcaattctc
     4021 gctctttctg acctgttgtt gcctgaaaaa gaaccgttcc tgcgtgtcca ctggcacaga
     4081 gccattgacc attgggggaa agtgccaggc gatcagcata gaattcgttg gcccaatctg
     4141 gttggccagt gtgaaggcgg ctgacctgaa gtccactcgc gccaccaatg gcgattccct
     4201 gaccatcagg gaatatagca agggatcgaa cacccggcag gcgcggtgac cagcgggttt
     4261 gctcctgacc ggtttggaga ttccagacgc tgaccgtgca atgctggcag ggcccgtgcc
     4321 agggacgcat atcattttga tcgccggcca gaaccagtgt gtttccatca accgagaagg
     4381 ccgccgcata aatcggtatc gccgagccct ggagccgaca cagttctcgt ccggttttcg
     4441 catcctgaag ctggcaaatc gagttgctcc aaactgtgag gagcaacagt gtttcatccg
     4501 gagaaattgc cagttcgtta tcccaggtgg agtgtttctt cacccggttg agttcaatcc
     4561 cggtttcaag attccaggtt cggatgaacc catcgtttcc cgttgaaaac aggcgctttc
     4621 catcttgtga aaaagtgagg gctgtcacgc ggtcctggtg cccggtgagt ttttttaccg
     4681 gggtattggc gccagtctcc cacaccagaa tctgcccatt ggtctggcct tgagccaggg
     4741 tttttccttc tggcgagaac gtgttgcaca aaatttcgtc ggttttggtg gtacgtcgct
     4801 gggtgaccgg cgtgccggtt tccaaatccc aggtcgtgac caggccggac tgatgaaccg
     4861 tcagcagatg gttgtggcgg gttgaaaagg agaattcttg tacatcggtt ctggttgtga
     4921 ggccaggtag atggagctcc tttcctgtag cgcaatccca aagctggagc ttgcccgatt
     4981 gaagcgttgc aatacggcct ccctccgaag aaaaccgtgc gtgatgaata ccctctgaag
     5041 aaacagccag ccggcgaatt tcctgacccg tgtgagcatc ccagagatga accgcgtaaa
     5101 g
//
LOCUS       DAOQDO010000107         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10571_length_5097_cov_3.844308, whole genome shotgun sequence.
ACCESSION   DAOQDO010000107 DAOQDO010000000
VERSION     DAOQDO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10571_length_5097_cov_3.844308"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKZ53_02750"
     rRNA            complement(<1..374)
                     /locus_tag="PKZ53_02750"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(713..2074)
                     /locus_tag="PKZ53_02755"
     CDS             complement(713..2074)
                     /locus_tag="PKZ53_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNJ39382.1"
                     /translation="MPKTTAFSFPPGPRRTFPGGNYLAFQRDPLHFFKNLSDKHGDLA
                     HVRLGMEDVIVVNNPDYIKEILVTNHQNFTKSRGLERAKNLLGEGLLTSEGEFHRRQR
                     RLSQPAFHRDRIASYGAVMVQRALQMRETWHEGVPIDVAQEMMHVTLAIVAKTLFDAN
                     VDSEADEIGEALTTSLELFVRFMVMPFGELIQKLPLPANKRFAKAKERLDATIYRIIE
                     ERRKSVEDRGDLLSMLLLAQDAEGDGTGMTNEQLRDEVLTLFLAGHETTANALTWTLY
                     LLSQNPEVETKLQAEVDAILNGRTPTVADMPQLKYAEMVLAESMRLYPPAWVIGRRNK
                     AAYQIGDYLIPPRSIFIMSQYVTHRDPRFFDQPEVFDPQRWTPEARSLRPKFSYFPFG
                     GGPRLCIGESFAWMEGILVLATIIQRWQLQLVAGHQVETQALITLRPKHGMKMCPKAR
                     IKK"
     gene            2397..3044
                     /locus_tag="PKZ53_02760"
     CDS             2397..3044
                     /locus_tag="PKZ53_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HNJ39383.1"
                     /translation="MVHRAAHYFFIRKLHSLSGIVPLGVFLGFHLYLNLHAASGPAAY
                     QRAIQTLQAFFPGPLLILTEIGLIVVPLLFHAGAGLYLTTHMKSNVVQYPHPQNWLYW
                     LQRMTGIMLLVFLLAHIATLRLGFGGPGVSVGANPDIAFEVVRFWLSQPFVMVFYGLG
                     IFSAAFHLANGLWSFAISWGITISTRSQQLFSYVCFGIGVLVFSLGVRVLLAFVN"
     gene            3061..4824
                     /locus_tag="PKZ53_02765"
     CDS             3061..4824
                     /locus_tag="PKZ53_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ39384.1"
                     /translation="MPKKPKVLIVCGETAGLMAALQLVHCGCEVELISPVPIRRLFAS
                     AQTGINAALNTKGEADSPVQHFEDTLVAGDFLAPQGQLKTMCAQAPELIWLLDRMGVP
                     FTRTTEGHIDLHRTPGTRFSRTAFVGTTTGPATLFALCEQIQRYESEGSITCYENWEF
                     LSAVIDETTCLGVIAMDLVTLEVKAFAAQAVVIAAGGVGGIFGRTTNAVHVTGSVQGT
                     LFRQGVRYVNAEFIQLHPATLIGEDKFRAISDLALAEGGRFWVPRTARDQRPPEEIPN
                     AERWYFLEEHYPGFGNFVPRDIATRGIFEISLDGYGVGSLSQVYLDLTDLSPTQLRRR
                     LEGIVDLYFQMTGEHASTIPMCVFPGMHLSLGGLWVNAEQQTNVKGLFAAGECAGSLH
                     GANALSGNELLFSLFSGVIAGQAAARCAQGTTSFPVEADPRFEAERRRQLDENEKLKA
                     RQGAENAWRIQEQLGRLMTEQMTVVRFNERLHGALEQIHQLEDRLARIDINETNFWAT
                     PSIPQARRLGSMLTLAQVMVVSALNRNESRGVHFKPEFPACNHTEYLKTTVATFSEAG
                     PVITFEPIDTSLIEPRPREYD"
BASE COUNT         1199 a         1279 c         1254 g         1365 t
ORIGIN      
        1 attccccact gctgcctccc gtaggagtct ggcccgtgtt tcagtgccag tgtggccgac
       61 cgccctctca ggtcggctac tgatcattgc catggtaagc cgttacctta ccatcaagct
      121 aatcagacgc aggctcctct cttggcgcga ggtcttgcga tcccccgctt taacaactgt
      181 ctcgataccg agccgctgca ttatgcggta ttagctcaag tttccctgag ttattcccca
      241 cctagaggta gattacccac gtgttactca cccggatgcc acgctactca gggttgcccc
      301 ctttggcgtt cgacttgcat gtgttaggca cgccgccagc gttgattctg agccaggatc
      361 aaactctcag tcaaaattgt gtgctcattg ctgagcgttt ttaactggtg acaggctcaa
      421 actggttaat tgacgagtaa aacttctgtc tagtttttca aagatcgtct tcttattttc
      481 gctcgccgtt tccgtgagcg aatggccagt atagggatga atcttttttt gtcaacactt
      541 tttttccgag ttttttcact ttttttgcgg cttttttttg aaacaaatct aaaatgttca
      601 tttcaaacaa cttataccca ggcacaaatt tcattaaaaa ctcgatctcg ttctcttttt
      661 gcctcatttt ttgaattcag tgtggttttt cgaccatttt cttcgaaaga aatcattttt
      721 tgattcgcgc ttttggacac attttcatcc catgttttgg gcgaagggtg atcaacgcct
      781 gagtttcgac ctgatgacca gccactaatt gcaattgcca gcgttgaatg atggtcgcca
      841 gcaccaaaat gccttccatc caggcaaagg attcgccaat acagagccga gggcctccgc
      901 caaaggggaa atagctaaat tttggcctca atgatctggc ttcaggtgtc cagcgttgtg
      961 gatcaaagac ctcaggctgg tcaaagaacc ggggatcccg atgggttaca tattgactca
     1021 tgataaagat cgaccttggt ggaatgaggt aatcgccaat ttgataggct gccttattgc
     1081 gtcggccaat cacccaggca gggggataga gccgcattga ctcggccagg accatctcgg
     1141 cgtattttaa ctgcggcata tcagccacag ttggtgttct tccgtttaag atcgcatcca
     1201 cctctgcctg gagtttggtc tcaacttcgg gattttgaga aagcaggtat aacgtccagg
     1261 tcagggcatt ggctgtggtt tcgtgaccag ccagaaaaag cgttaaaacc tcgtcccgga
     1321 gctgttcatt cgtcattcca gtaccgtcac cttcggcatc ctgggccaat aaaagcattg
     1381 aaagtaaatc tccccggtct tccacgcttt ttcgacgctc ttcaataatt cgataaatcg
     1441 tggcatccaa ccgctctttt gctttcgcaa agcgtttatt ggctgggagc ggcagctttt
     1501 gaatcaattc accaaacggc ataaccataa atcggacaaa taattcgagc gatgtggtca
     1561 gggcttcccc gatttcatcg gcttcggagt caacattggc atcaaaaagc gtctttgcca
     1621 caattgccag cgtaacgtgc atcatttcct gagcgacatc gatgggaact ccttcgtgcc
     1681 aggtctctcg catctggagt gcccgttgca ccatcaccgc gccatatgaa gcgatccgat
     1741 ctcgatggaa tgctggttga gacagccgtc gttgccggcg atgaaactct ccttcgctgg
     1801 ttaacagtcc ctcaccaagc aggttcttcg cccgttccag gccgcggctt ttggtaaaat
     1861 tctggtgatt tgtgaccaga atctctttga tatagtcggg gttgttcacc acgatcacat
     1921 cctccattcc gagccggacg tgagccaaat ctccatgttt atctgagaga tttttaaaaa
     1981 agtgcaatgg gtcacgttga aaagcgagat agtttccacc aggaaaggtc cgcctcggac
     2041 caggtggaaa tgaaaaagca gttgtttttg gcatagcgtg tttaaaacct gtgtgatgaa
     2101 aaatggaaaa gcattcaaat gggtaaagtt tagccgtgcc atcagttgaa ataccagata
     2161 ttgatatcct gatgggcaca aactcgctga ttttcccgcg tgaggaagag atgagtggca
     2221 aaagaatctc ttttttgcaa agtgatacga tctcgacacc ttgaacccct gatggaattt
     2281 actttggctc agccactcct ttattgttcc ggtgaaaaga gtggaaccca ggcacatccc
     2341 tctttttctc aaaagactca actgaacccg ccttccttcc acctgaaaga ttcatcatgg
     2401 tacatcgtgc ggcacactac tttttcattc gaaaactgca ctcgttgagt gggattgtcc
     2461 cactgggcgt ctttttgggg tttcatctct acttgaattt acatgctgcc agtggaccag
     2521 ccgcctatca acgtgcgatc cagacccttc aagccttttt ccctggccca ctccttatat
     2581 tgactgaaat tggcctgatt gtagtaccac tgctgtttca tgccggggct ggtctgtatt
     2641 taacgaccca catgaaaagc aatgtcgttc agtacccgca tcctcagaac tggctgtact
     2701 ggctccaacg gatgacaggc attatgttgc tggtgtttct cctggcacat atcgcgacgc
     2761 tccggctagg ctttggaggt ccaggagtga gtgttggcgc caatccagat attgcctttg
     2821 aggtggttcg cttctggctc tcacaaccct ttgtgatggt attttatggg ctgggcatct
     2881 tttcagccgc ttttcatttg gcaaatggcc tctggagctt tgccatttcc tggggaatca
     2941 ctataagtac tcgatcccag caactgtttt cgtatgtgtg ttttggaatt ggagttctgg
     3001 tgttcagcct tggagtccgt gtattgcttg cttttgtgaa ctagcttgac caggtaagat
     3061 atgccgaaaa aacccaaagt ccttattgtc tgtggcgaaa ccgctggcct gatggctgcg
     3121 ctccaactgg tccactgcgg ctgtgaagtt gaactcatct ccccagtccc gattcgacga
     3181 ctttttgcaa gtgcgcaaac ggggatcaat gccgccctca ataccaaagg tgaagcagac
     3241 agtccagtcc agcattttga ggataccctg gtggctgggg actttcttgc acctcaaggc
     3301 cagctcaaaa caatgtgcgc ccaggctccc gagttgatct ggctgctcga ccgaatggga
     3361 gtgcccttca cgcgtaccac tgaaggtcac attgaccttc atcgaacgcc gggcaccaga
     3421 ttctcacgga ccgcttttgt cggaacgacc accggaccag ccaccctgtt tgccctgtgt
     3481 gaacaaatcc aacgctatga aagtgaagga agcatcacct gttatgaaaa ctgggagttt
     3541 ctctctgcgg tgatagatga gaccacctgc ctgggtgtca tagcaatgga cctggtgacg
     3601 cttgaagtca aagcgtttgc ggcccaggca gtcgtgattg cagccggcgg ggttgggggc
     3661 atttttggaa gaacaaccaa tgcagttcat gtgacaggga gtgttcaggg aacactcttt
     3721 cgacagggcg tccggtatgt caacgctgaa tttattcaac tccatccagc aacattgatt
     3781 ggggaagata aattccgagc catatctgac ctggccctgg cggaaggcgg gcgattttgg
     3841 gtaccccgca ccgcacgtga ccagcgtcca ccggaagaga tcccaaatgc tgaacgctgg
     3901 tattttcttg aggaacatta tccaggcttc gggaattttg tccctcggga cattgcgacg
     3961 cgaggaattt ttgaaatttc gctcgatgga tatggcgtcg ggagtttgag tcaggtgtat
     4021 ctggacttga ccgatctttc accaacccaa ctccgccgcc gactggaagg aatagttgat
     4081 ctttacttcc agatgactgg cgaacatgct tcgacgattc caatgtgtgt ttttccagga
     4141 atgcatttaa gcctgggagg attgtgggtc aatgccgaac agcagaccaa tgtcaaaggt
     4201 ttatttgctg ctggtgaatg cgctggatcc ctccacggtg ccaatgcact gagtgggaat
     4261 gaacttttgt tcagtctttt ttcgggagtc attgcagggc aggcagcggc ccgctgtgcc
     4321 cagggaacaa catcgtttcc agtggaagca gacccccgct ttgaagctga acgacgacga
     4381 cagcttgatg aaaatgaaaa gctgaaagcc cggcaaggcg ctgaaaacgc ctggcgtatc
     4441 caggaacagt tgggtcgcct gatgactgaa cagatgacgg tggttcggtt taatgaacga
     4501 ttgcacggcg ccctggagca aattcaccaa ctcgaagacc gtctggcccg gattgatatc
     4561 aatgaaacaa atttttgggc aacaccttcg attcctcagg ctcgtcgcct gggctccatg
     4621 ttgacactgg cacaggtcat ggtggtcagc gccttgaatc gaaatgaatc gcggggcgtt
     4681 catttcaagc ctgaatttcc agcgtgtaac cataccgagt atttgaaaac tacggtggcc
     4741 accttctcag aagcgggccc cgtcattacc tttgagccga ttgacaccag cctgattgag
     4801 cctcgcccac gtgaatacga ctaaactaca tggccaccag gatgggtcac gtggcttgga
     4861 agctcaccgg cttcctggta cagtaaaatg tgtctcaggt tcagaaaatc tgggatttcc
     4921 aaaaatctga aatcagaaaa gatcaattga ttcttatatt tgccatgctc gaagcatcga
     4981 gcggatcagc ggcaccaagg aggaatgcat gaggaaggat ttacaaccac catcacagaa
     5041 tcgccaaaag acaggtctgt ggttgaaatt tgggattttg gccgtaacag ccggcct
//
LOCUS       DAOQDO010000108         5095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10576_length_5095_cov_2.784524, whole genome shotgun sequence.
ACCESSION   DAOQDO010000108 DAOQDO010000000
VERSION     DAOQDO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10576_length_5095_cov_2.784524"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..665)
                     /locus_tag="PKZ53_02770"
     CDS             complement(24..665)
                     /locus_tag="PKZ53_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39385.1"
                     /translation="MRSVKVQLVQFAIAAVVVLACFVPSFAQAGTATLAGGKTSVTLS
                     TDFVGDLTTLGVAPDRVSPAKLKRGIASFPIPSGALDLSTAKGEIDHTGGLTLTAGST
                     RVQLLNFVIDTTGATPVLTGLVVVNGTLLGRLPLFDLALPALTLPIQPGANGRVTIGN
                     VGVTLTETAAGALNQVFGVTAFVKGFNIGTAVVDATPGAPKTGKRAAETESEE"
     gene            complement(971..1186)
                     /locus_tag="PKZ53_02775"
     CDS             complement(971..1186)
                     /locus_tag="PKZ53_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39386.1"
                     /translation="MLDLFLALSSKIVLEISEFERSYLVFVWHLSGIYRGKMMVGKMG
                     KKEESELNRVEISSESLNKSPRRLMSG"
     regulatory      1214..1408
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1485..1970
                     /locus_tag="PKZ53_02780"
     CDS             1485..1970
                     /locus_tag="PKZ53_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39387.1"
                     /translation="MNQPATICVTVLSIPQLTGADHSQGLCRLQVESPHPLPRAPYAG
                     TVRVYGLETTRTGNHQCRMLKHFTIEVAEELPAGLIYQVRVDLADELPAPIPPPSLPS
                     FHSTSYPSVHQPLVSLPAIVAPDLGRRRVTEPLETICKRIAGIFGRYCEEFRAPSISV
                     R"
     gene            2084..4021
                     /locus_tag="PKZ53_02785"
     CDS             2084..4021
                     /locus_tag="PKZ53_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ39388.1"
                     /translation="MEGPLLFLLVFSVIAIVILLATINPFYLIRESEVGLVEKKIIGK
                     SLTPGRVIATGEQGLQARVLPPGLHFLPKPFFKVTRIPITEVPQGQMALVTAIDGAPL
                     KTGQLFAESAEGHTSFQDGEAFLRRGGQKGPQLEVLTPGRYRINTYLFNVQPATMIEI
                     PQGKVGIVTATDGAMLQTGQLLGQHVQGHQNFQNSQVFLRAGGQKGPQVDILTPGQYR
                     INTQLFRIQLADAVRVKTGEIGLVSALDGSPLREEQILGDTVTGHNDFQDGQAFLNHA
                     GQRGPQLDILKPGMYYVNPLLFQVTVQEATVINIGEVGVVRSNVGILPPVGEDGRTPD
                     LVDEGFRGVLKSVLEPNTYYLNPIAYVVFPISTLNITIDWNTTNRATPLDSITVKSRD
                     GFSFPVDVKVVIRVLKEKAPVVVSKIGSIQNAIDRVIHPAIGATFRNNAERCDALDFL
                     NNRSLQQANAREVTTQMLREYGIETVDVLIGDVGIPETLLKTQTDRFLAREREKTYRE
                     QKTAEETRSALENATASADQQKHIVAAQAAVEIAKSQAAAAIATAEGEAKQITLRAEA
                     NQHAYKLLISEIGREGVLTIEMLKLIKEGKIKITPDVFVSGGGEGKNTALSALAATML
                     NGQVTALNNNQPMAIVEKSVA"
     gene            4725..>5095
                     /locus_tag="PKZ53_02790"
     CDS             4725..>5095
                     /locus_tag="PKZ53_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39389.1"
                     /translation="MPNKTLKILKESLPTRCEVCHQSDLFDPVTGLCGRCQAIVQMDA
                     RGIESLRIACPRCGTTTEYWETIEYCYHCLGFTGRGVVPTSVRPTKARQMSAEEFEQR
                     IGQWLGCLLLIIFLCVLGVWS"
BASE COUNT         1270 a         1302 c         1265 g         1258 t
ORIGIN      
        1 gataccctca gccctagatt aaattactct tccgattcgg tttcggcagc ccgtttcccg
       61 gtttttgggg cacccggtgt ggcatcaacc accgctgtgc caatgttgaa tcctttgaca
      121 aatgcagtca cgccaaagac ctgattgagc gctccggctg cggtttcggt taatgtcact
      181 ccgacgtttc caatcgtcac ccgtccattt gcccctggtt gaatgggtaa ggtcaaggct
      241 ggcagggcaa ggtcaaagag tggtaaccgt ccgagcaacg taccattgac caccaccaga
      301 cccgttagaa ccggagtggc gccggttgta tcaatgacaa aattcagcaa ctgcacccgc
      361 gttgagccag ccgtcaacgt caaaccgccc gtgtgatcaa tttcaccttt ggccgtgctc
      421 agatcaagcg cacctgatgg aattggaaat gaagcaatgc cgcgtttgag cttcgccggt
      481 gatacccggt ctggtgcgac gcctaaagtc gtcaagtctc caacaaaatc agtactcaac
      541 gtcacgctgg tcttcccacc ggccagcgtg gccgtgcccg cctgggcaaa tgaaggaacg
      601 aaacaagcca gaaccacaac ggctgcgatt gcaaattgaa cgagttggac ctttactgaa
      661 cgcataagaa aaaatctcct gatatgtgtg tttttaatct ttcttttttg ttttcggtgg
      721 gaatgaacat tcgctctaga tgaaagcgtg tcgagcacct gtcatcacag cagaaagcac
      781 ctcgacaatc catagacaag ttaatgtaaa tgagttgcgt gtaaatatct cttgtggatg
      841 ctcgacgtgg ctgatttcgt ctggatgtga aaactggatg caaaaatctt ccggattttt
      901 attttttctt ccgaatccaa aataagttct ttttttataa tattttacat atgatttatt
      961 tttccgtatc tcaccctgac atcaatcttc tgggggattt attcaaactt tcagatgaga
     1021 tttccacacg attgagttct gattcctctt tttttcccat ctttcccacc atcatttttc
     1081 ctcgataaat accagacaaa tgccagacaa acaccagata acttctttca aattcagaga
     1141 tttctaatac gattttgctt gacagtgcca aaaaaaggtc tagcataggt atcagttttt
     1201 gaggttgttt gtcagaactt tgcagccgag tctactttgc aacgaaagaa gtagagcggg
     1261 ggaaccaact ggaagccagt catcagcctg tgtgatgccg gctttctggg gtgaatctgg
     1321 attgatcttc agcaatgagt gatctccagt agggcacttc caagcccgaa tccgtcagct
     1381 aacctcgtag gcacgtttgg aagggcgaac cgatctgcgt gtcaaagaga tgaagaccac
     1441 agaccgtttg ggcctgtggt cttttgcttt ttggcgaaac acatatgaat caaccagcta
     1501 cgatttgtgt cactgtgctt tcgattcccc aattgacggg ggcagaccat tcacagggac
     1561 tgtgtcggtt gcaggttgaa tcaccccacc cacttccacg cgcgccatac gcaggcactg
     1621 tcagagtcta cgggcttgaa acgactcgaa ctggaaacca ccagtgccgg atgctcaagc
     1681 acttcaccat cgaagtcgcc gaagaattac cagcgggcct gatttatcag gtcagggttg
     1741 atttggcgga tgaacttcca gccccgattc ccccaccttc acttccttca tttcacagta
     1801 cctcataccc atcggttcat cagcccctgg tgtctttgcc ggcaatcgtc gcgccggatc
     1861 ttggtcgccg tcgcgtgact gaaccactgg aaaccatctg caaacgcatt gctggcattt
     1921 tcggtcgcta ttgcgaggag tttcgggctc ccagcatcag cgttcgatag acctcgacag
     1981 tgaggaatga gggccgaaag acgcattagg caaggactca ggacaaacct tttgcccctc
     2041 attccttttc ttttcaccct ggtttgctct tgggagactg cgtatggaag ggccactttt
     2101 gttcttgctg gtattttcag ttattgccat tgtgatattg cttgccacca tcaatccgtt
     2161 ttatctcatt cgtgaaagcg aagttggact ggtcgagaaa aaaattattg gaaaatcact
     2221 tactccaggg cgggtgatcg ccactggcga acagggttta caggcccgtg tgttgccccc
     2281 aggattgcac ttcctgccca aaccgttttt taaagtcacc cgcattccga ttaccgaagt
     2341 cccccagggc cagatggcgc tggtcaccgc gattgacggc gccccactca aaactggtca
     2401 gctttttgcc gaaagtgccg aaggccacac cagttttcag gacggtgaag ccttcctgcg
     2461 ccggggcgga caaaaagggc cgcaattgga agtcctgaca cctggacgct accggatcaa
     2521 tacctatctg tttaatgttc agccagccac catgattgaa attccgcaag gcaaagttgg
     2581 tattgtcacg gcgacggatg gcgcgatgct ccagacaggt caactgttag gccagcacgt
     2641 ccagggccat caaaactttc aaaacagcca ggtctttttg cgagctggag gccaaaaagg
     2701 cccccaggtt gacatcctga caccgggtca atatcgcatc aacacccaat tgttccgcat
     2761 tcaactggcc gatgccgtac gggtcaaaac cggtgaaatt gggctggttt cagcccttga
     2821 tgggtcaccg ctccgcgaag aacaaatcct gggcgatacc gtcaccggcc acaacgattt
     2881 tcaggacggc caggcatttc tcaaccacgc cggccagcgc ggaccgcaac tcgacattct
     2941 caaaccgggg atgtactacg tcaacccgtt gctctttcag gttacagtcc aggaagccac
     3001 cgtcatcaac attggcgaag tcggcgtggt tcgttccaac gtcgggattt taccgccagt
     3061 tggtgaggat ggccgcacgc cggacctggt ggacgaaggc ttccgtggcg tgttgaagag
     3121 tgtgctggag ccaaatacct attacctgaa cccgattgcc tatgtggtgt ttccgatctc
     3181 aacgctcaac atcacgattg actggaatac caccaaccgc gcgacaccgc ttgattcgat
     3241 cacagtcaag agccgcgacg gtttctcatt tccggtggat gtcaaagttg tgattcgggt
     3301 tctgaaagaa aaagcgccgg ttgtggtctc caaaattggt tcaattcaaa atgcgattga
     3361 ccgcgtgatt cacccggcaa ttggggcaac cttccgcaac aatgccgaac gctgtgacgc
     3421 gctcgacttt ttgaacaacc gttcgctcca gcaggccaat gcccgcgaag taacgaccca
     3481 gatgctgcgt gaatatggta ttgaaaccgt tgatgtgttg atcggagacg ttgggattcc
     3541 ggaaacgctg ctcaaaaccc agacggaccg cttcctggct cgcgaacggg aaaaaaccta
     3601 ccgcgaacag aaaactgccg aagaaacccg gagcgccctg gaaaacgcca cggcttcggc
     3661 tgatcaacaa aaacacattg tggcggctca ggcagccgtc gaaattgcca aatcgcaggc
     3721 agcggcggcg attgcaacgg ctgaaggcga agccaaacaa attacactcc gtgctgaagc
     3781 caatcagcat gcctacaaac tgctcatctc ggaaattggt cgtgaaggcg tcctgacgat
     3841 tgaaatgctc aaactgatca aagaaggcaa aatcaagatc accccagatg tgtttgtcag
     3901 cggtggcggc gaaggcaaaa acacggcgct ttcggctctg gcagcaacga tgctcaacgg
     3961 gcaggtcaca gcactcaaca acaaccagcc aatggcgatt gtagaaaaat ccgtagcctg
     4021 aagcctgggc tgaagacatc gggctgaaga cggcgggatt ggggtttagg gctgaagata
     4081 tcgggctgaa ggttcgattc ttcagcccga atttatttta ccttcgttga cttacccttg
     4141 ttttacataa acaattaaat aggtgtcaga attgaacctg gagtgaacta tgagttcaaa
     4201 tgctcatctg gttatcaaga aagagaccct ccccacccgg tgtgaaatct gtcatcaagc
     4261 cgatcaattt gatccagtcg ccggggtttg ccagcgctgc cagtttgtat ttggaggaaa
     4321 ctccgaaggt aatcccctgg cacagtcccc atatttgacc tgccccaaat gcggaagttt
     4381 aggaaaattc ttcgcttcgg ttggtttttg ctaccagtgc tatcaacaaa cagcggtccc
     4441 gactggcagg ccgattcgct atggtccggt tgtttcaaga agcaacatat ggtcaccacc
     4501 taagcgcaaa ccaaaagcat cggttgagaa cattcgtgat aggacaatca ccctcaagcc
     4561 actttataaa caactggcac ttgggatatt cgcccttatt gtctatggtg gattgacagg
     4621 attgataaac cccgtgttgg tttggattgg tttttggatt ggactttgca ttctgtgctc
     4681 ctgggataga tgaatccaaa ataggtgaat ggaaccaaca ggttatgccc aataaaacct
     4741 tgaaaatcct gaaagaatca ctcccgactc gatgcgaggt ttgccatcag tctgacttgt
     4801 ttgatccagt caccggtttg tgtgggagat gtcaggcaat tgtgcagatg gatgcacggg
     4861 ggatagagtc actcaggata gcttgcccac gatgcggaac aaccacggag tattgggaaa
     4921 ccattgaata ttgttatcac tgtctaggct ttactggacg aggggttgtt ccaacttcgg
     4981 tacgaccaac gaaagctcgt caaatgagtg cggaggagtt tgagcagcgg attgggcagt
     5041 ggttggggtg tttactactg atcatttttt tatgcgtgct tggcgtatgg tcttt
//
LOCUS       DAOQDO010000109         5091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10591_length_5091_cov_2.899921, whole genome shotgun sequence.
ACCESSION   DAOQDO010000109 DAOQDO010000000
VERSION     DAOQDO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10591_length_5091_cov_2.899921"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..982)
                     /gene="hemB"
                     /locus_tag="PKZ53_02795"
     CDS             complement(2..982)
                     /gene="hemB"
                     /locus_tag="PKZ53_02795"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNJ39390.1"
                     /translation="MAHLIERPRRNRRTDAIRRMVRETAITPSDFVAPLFVMDGTDTR
                     IPIASMPGCYRLTIDLLVAECKELAALGIPGVALFPALPDSKKDKRGTESVNPDGLFA
                     RSIQAVKESVPDLMIFTDVALDPYSSDGHDGIVNDRGEIVNDETLDVLAQMAVAQARA
                     GADFVAPSDMMDGRVGAIRQALDQEGFINVGIMSYAVKYASAFYGPFRDALDSAPKFG
                     DKKTYQMDPANAREALREVRLDIAEGADMVMVKPALAYLDVIRLVRGAVDVPVTAYQV
                     SGEYAMVKAADERGWIDGQRCMLEILTAIKRAGADVIFTYFSKEAAQLLS"
     gene            complement(1080..1553)
                     /locus_tag="PKZ53_02800"
     CDS             complement(1080..1553)
                     /locus_tag="PKZ53_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39391.1"
                     /translation="MHQFTLDPSSLKIIDAHLDTCFPELRQPGQPALPPIVVSGGWLA
                     WYLTWVAKADGITFGRYIFLRPRIFRQSLATERVLETLGPLVVHEATHVWQYRRDGTL
                     PFLVNYVGAYLRNLTRSGVWWVLSAQTRHQAYLAIPYEVQARHAEAMWHVFHPSA"
     gene            1823..2359
                     /locus_tag="PKZ53_02805"
     CDS             1823..2359
                     /locus_tag="PKZ53_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39392.1"
                     /translation="MRAKFSRFAVLLLVCVIGFGSINSLAGSGQIQFKRPKDADPLLE
                     NPQTFAKSREEMMDLIKQTLKDMEIPLDEKRTTEQTGMLYTKPVIFTKGGPSGENLEF
                     VSRRPGGQLRNWTRGRYTLQIEIAPVDPQRIKLFVFAQIEGEYQDDALGAKWVTCPSR
                     GVIESNTLQSFKDRVGEQ"
     gene            2476..4200
                     /locus_tag="PKZ53_02810"
     CDS             2476..4200
                     /locus_tag="PKZ53_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39393.1"
                     /translation="MSEYLLTNLALVSGWVWGSLAAVIIITFFVLMVVALRRAFRNVD
                     QIEGLDTHTGSPLSLRSSLTPPATTGDTLPLTSEGPSLEDQVPITDEIPIPALGPILN
                     QSPQPVSLSEAETMAASAEPPAYVPTAPVPVGFHNLPTTTADMATPLTEPLSAGYPTG
                     PLEEDVETTEENVIEVQPRAHGSLLAQQIAQQIAERRAKLTGQEVGASPTVNLEKSTS
                     RATEGLKVESETQPLMSPGPIVFPPEPPVLPKAAPGAPPQLEKLPSSAPRKLLEIPER
                     LPSNARKTLQPPPTSPISQPRPTEPQSTSLPAQIAQAMSASSQNLAPAPPEPAYVGSS
                     LSPTLPAPHPPMAATLPLTARITNGHATVSDEVLPPSFLDSEARYGSGDIKDERQWAI
                     YLVLGFISLLGIGLVVFFPSVRNRVLPAKVAAMVQKTPIWLEREAPPPPPVPPTLLEI
                     HQFEPGLKPTAVKEVTTVIIGGTVKNISSAPLRNIRAEIRLVKRGTEETPPEIRQIGV
                     VPVDLMPGAEGRYVLEFLNKDYQSWRFNRLVLEDGTEAKSKDLERIAVPVELMAPPPG
                     EKTSKAKK"
     gene            complement(4387..5082)
                     /locus_tag="PKZ53_02815"
     CDS             complement(4387..5082)
                     /locus_tag="PKZ53_02815"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNJ39394.1"
                     /translation="MASDFPIKKELIADVRFARARERMVALLCERGISNEAVLKAMGT
                     IPRELFLDPVLSGESYGDHALPILAGQTISQPYIVARMTELLEPLPTKRVLEIGAGSG
                     YQTAILSLVVGKVFSIERIAALARLAQSRLQQLGIYNATVKCFDGTVGWSQFAPYGGI
                     LVAAAGPEIPQPLVDQLAVGGHLVLPVGDENAQRLIRVTRTPEGLRQEDHGGVKFVPL
                     IGRHGHKEPEAAK"
BASE COUNT         1255 a         1385 c         1300 g         1151 t
ORIGIN      
        1 cttaactcag aagctgggcg gcttctttcg aaaagtaggt aaagatgacg tcagcccctg
       61 cacgtttaat ggccgtcagg atttccagca tacagcgctg gccgtcaatc cagccccgtt
      121 catcagcggc tttgaccatg gcgtactcgc cgctgacttg ataagccgtc accggaacat
      181 caaccgcgcc acgcaccaat cgaatcacat cgagataggc aagggccggt ttgaccatca
      241 ccatatcggc gccctcagca atgtcgagtc ggacttcacg cagggcctcg cgggcattgg
      301 ccgggtccat ctggtacgtt ttcttatcgc cgaatttggg agctgaatcg agggcatcgc
      361 ggaacggacc gtaaaaagcc gaggcgtact tcacggcata ggacatgatg ccgacattga
      421 taaacccttc ctgatcaaga gcctggcgga ttgccccgac gcggccatcc atcatatccg
      481 atggggccac aaagtcagcg ccagcgcgtg cctgggcgac ggccatttgg gccagaacgt
      541 caagcgtttc atcattgacg atttcacccc ggtcattaac gatgccgtcg tgaccatccg
      601 aagaatatgg atccagcgcc acatccgtga aaatcatcag gtcaggaacg ctttccttga
      661 cggcctgaat cgaacgggca aagagaccat ctggattgac actttctgtg ccacgcttgt
      721 cttttttgga atccgggagc gccggaaaga gcgccacgcc aggaatgcca agtgcggcca
      781 gttccttgca ctcagccacc agtaaatcaa ttgtcagccg ataacatccg ggcatcgatg
      841 caattggaat gcgggtgtca gtgccgtcca tcacaaacag tggggcaaca aaatcggaag
      901 gcgtgatcgc cgtttcgcga accatgcgcc ggatggcatc cgtgcggcgg ttgcgtcgcg
      961 gacgttcgat caagtgggcc ataacatgct cctgaaatga agtgggtttc ctggaaacag
     1021 ggagggaatc tgcaactcta tccgaaagtg tccggctctg ccacaacttc gcgcctgttt
     1081 caggctgatg gatgaaaaac gtgccacatc gcttcggcat ggcgagcctg gacttcatag
     1141 ggaattgcca gatatgcctg atgccgggtc tgggcgctca gcacccacca caccccggac
     1201 cgggtcaggt tgcgcaggta cgcgccgaca tagttcacta aaaagggaag ggttccatcg
     1261 cggcggtatt gccagacgtg ggtggcttcg tgaaccacca gcggtccgag cgtttcaagg
     1321 accctttcgg ttgccagcga ttgccggaaa atccgaggcc gtaaaaaaat atagcggcca
     1381 aaggtgatgc catcggcttt ggcaacccag gtcaaatacc aggccagcca gccacctgaa
     1441 acgacaattg gtggtaatgc tggctgtccg ggctgtcgca gttcaggaaa acaggtgtcc
     1501 agatgggcat caataatttt caaagaggac ggatcgagtg tgaactggtg catcgtgaaa
     1561 actccggttg aaaaacaagg caacgtcagg ccgccgcagg ctaaatttgc cgaaacacac
     1621 agacatttcc aacttaattc agttaagatg tgtccaagtc aaagttctca aataaactat
     1681 caatcacaat cccactcaat cctgactggt tgttcggttt cctggaaacg tgtttcctgg
     1741 taaaaccaga gtgtcaatct taccccctat cctgttggat gagctggatt tttatctttt
     1801 gaattttttg gagattatcg ctatgcgagc aaagttttcc cgatttgcag tgctgctgtt
     1861 ggtttgcgtg atcggttttg gttcgatcaa tagcctggct gggagcggtc aaatccagtt
     1921 taagcgtcca aaggatgctg acccgttgct ggaaaaccca caaacctttg ccaaatctcg
     1981 cgaagaaatg atggatttga tcaaacagac cctcaaggat atggaaattc cgctggatga
     2041 aaaacgcacc accgagcaaa cggggatgct ctataccaaa cctgtcatct ttaccaaagg
     2101 tgggccgtct ggtgaaaacc ttgaatttgt ttcacggcga ccaggtgggc aattacgtaa
     2161 ctggacgcgt ggacgctata cgctccaaat cgaaattgcc ccagttgatc cgcaacgcat
     2221 caaacttttt gtttttgccc aaattgaggg cgagtatcaa gacgatgccc tcggggccaa
     2281 gtgggttacc tgtccgtcac ggggcgtgat tgaaagcaat acactccagt catttaagga
     2341 tcgtgttggc gaacaatagc cagcacgtca cttcagataa caggttgacg aatatatagt
     2401 tttttttctt cggagaagaa aggcagcact ttgggggcct ccatacaggt tgcacagaga
     2461 agtgctgagg agcacatgag cgaatattta ttgacgaatc tggcattggt gagcggttgg
     2521 gtgtggggaa gtctggcggc agtgatcatt atcacttttt tcgttttgat ggtggtggca
     2581 cttcgccggg catttcgcaa tgttgaccaa attgaagggc tggacactca caccggcagt
     2641 ccattgagcc tgagaagcag cctcacaccg ccggcaacga ccggagacac gcttccattg
     2701 acatccgaag ggccctctct ggaagatcag gtgccgatca ccgatgaaat cccaatccca
     2761 gctcttgggc cgatcctcaa tcaatcgcca caaccagttt ccctctcgga agctgaaacc
     2821 atggcggctt cggctgaacc accagcttat gttccaactg ctccagtccc cgtcgggttt
     2881 cacaaccttc ctacgaccac agcagatatg gccacccccc tgactgagcc gctctcggca
     2941 ggctatccca ccgggccgct tgaagaagac gtcgaaacca ctgaagaaaa cgtgattgaa
     3001 gtgcaaccac gagcccacgg cagcctgctg gcgcagcaaa ttgcccagca aattgcggaa
     3061 cgccgcgcca aactgactgg tcaggaagtc ggcgcctcgc caactgtcaa ccttgaaaaa
     3121 tcaacgtcac gggcaactga aggattgaag gttgaaagtg aaacccagcc actgatgtca
     3181 ccgggaccga ttgtttttcc gcctgaacca ccagttttac caaaagcagc accgggcgct
     3241 ccgccccaac tcgaaaagtt gccgtcatct gcaccgcgaa aacttctgga aatccctgag
     3301 cggctacctt caaatgcccg caaaacgtta cagccaccgc caacgtcacc aatttcgcaa
     3361 ccccggccaa ctgaacctca gtccacatcg ctccccgccc aaattgccca ggccatgagt
     3421 gcaagcagcc agaatctggc acccgcacca cctgaacctg cctatgttgg ctcgtcgttg
     3481 tcgccgacgc ttccggcacc acatccacca atggcggcca ctctgcctct gactgcccgg
     3541 atcaccaatg ggcacgctac tgtgtcggat gaagttcttc ctccttcatt tttggattct
     3601 gaagcccgtt atggaagcgg tgatattaaa gatgagcgcc aatgggcaat ttatcttgtt
     3661 ctcggattta tcagcctgtt agggattggg ttggtggtat ttttcccatc agttcgcaat
     3721 cgtgttttgc cagcaaaagt ggccgccatg gttcaaaaaa cccctatctg gttagaacga
     3781 gaagccccac ctcccccgcc tgtgccaccg accctgctgg aaattcatca atttgaacca
     3841 gggttgaaac ccaccgctgt caaagaagtt accaccgtga tcattggcgg gaccgttaaa
     3901 aatatttcgt ccgcgccatt acgcaacatc cgggcagaaa ttcggcttgt gaagcgcggc
     3961 actgaagaaa caccgcctga aatccgccag atcggcgtgg tccccgttga cctgatgccc
     4021 ggtgctgaag gccgttatgt gcttgagttt ctcaacaaag attaccagtc ctggcggttt
     4081 aaccggctgg tgctcgaaga cggaactgaa gcgaaaagta aggatctgga gcggattgcg
     4141 gttccggttg aactgatggc cccaccacct ggcgagaaaa ccagtaaggc aaagaagtga
     4201 tgcgaatttg cagtcagttg tcagtcgtca gtcgtttgct aagtttcgtt ggttaaattc
     4261 cttgactgtt tctgatatga ggattttatt gcagattggt gtcccgaaca ggaaaatgaa
     4321 gagataaaaa caaaaggaga cagaagtttt tcttctgtct ccttttgttt gactactcgc
     4381 tattcactac ttcgctgctt caggttcttt atggccatgg cggccaataa gcggcacaaa
     4441 tttgacgccg ccgtggtctt cctgccggag cccctctggc gttcgtgtca cgcgaatcaa
     4501 tcgctgcgca ttttcatccc cgactggtaa aaccagatgc ccgcccactg ccagttgatc
     4561 taccagtggt tgaggaattt ccggcccggc agcagccacc agaatcccac catagggcgc
     4621 aaactggctc catccgaccg tcccgtcaaa gcatttcacg gtggcattat agattccaag
     4681 ttgctgcaat cttgattgag caagccgggc aagggctgca atccgttcaa tcgaaaagac
     4741 ttttccgacg accagactca aaatagcagt ctggtagccc gatccagcac caatttcaag
     4801 cacccgtttg gtgggcaatg gttcgagaag ttcggtcatg cgagccacaa tgtacggttg
     4861 ggaaatcgtc tggccggcaa gaatgggcaa tgcgtggtcg ccataacttt cgcctgaaag
     4921 tactggatct aaaaacagct cccttggaat tgttcccatt gctttcaaca ccgcttcatt
     4981 ggagatgccg cgttcacaca agagcgcgac cattcgctcg cgtgcccggg caaatcgaac
     5041 atcggcgatc aattcttttt tgattggaaa atcagaagcc atgagtgttt t
//
LOCUS       DAOQDO010000110         5054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10704_length_5054_cov_3.248650, whole genome shotgun sequence.
ACCESSION   DAOQDO010000110 DAOQDO010000000
VERSION     DAOQDO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10704_length_5054_cov_3.248650"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKZ53_02820"
     CDS             complement(<1..917)
                     /locus_tag="PKZ53_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39395.1"
                     /translation="MFRSSLSLMTRPNLRSNPQLAGVIPINEALEHKVLVLGVTSQEL
                     KAAVVDDAGSAYLTQVMHQLDFQRSLARAARLTLPEVSRLVLKTSRHAVEDVLKGISN
                     SYPKAMELRFQAAVATVIPRETAERHAILIECLVGTTIHALYADGNGRQTLEFLLNRA
                     DIRTAIAVAAQVDERLAGEFRCEPRRVPREVIDREIKRLYAETRYEGFTQRDTRRYVQ
                     AVKREWATQAVSTPGRPKTEAPQSFSHTRQVLYDIAGSAFRQEASDVRIFYDWDRRAI
                     QHELFLDGEWRRQAEIPLDFAVYQSMINLA"
     gene            complement(1148..1723)
                     /gene="pilO"
                     /locus_tag="PKZ53_02825"
     CDS             complement(1148..1723)
                     /gene="pilO"
                     /locus_tag="PKZ53_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNJ39396.1"
                     /translation="MLVRRIVTQGIIAVLMAASGWVVADQFWFSEWQSKLNTQQDVIA
                     RQTKENDITDRYRQEHDQLVAKVEATRDQYRQVEKYLPSVDGLGELFEQVKNQARAAH
                     LVIERKDDMHAPYKPGINESRLVIQLRGSFLNHLKFLEWMGNHPRLLVFSEQEVRSTD
                     GTTTMTLKGFAPVDVQTKEVAEATNTILAKG"
     gene            complement(1866..3410)
                     /locus_tag="PKZ53_02830"
     CDS             complement(1866..3410)
                     /locus_tag="PKZ53_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNJ39397.1"
                     /translation="MLGVIRFGYQSAGVTLGIVKTDGKSGPTLVHLEQLASQADAVAT
                     LQSIASDIQVLTIYPSPVEVRPGEAFMTDLASSVALSNLVTARCDFDATLMLAYLDPM
                     IVEHLPVGSVLCEAACLAEAFTTVYGREAEQAVRAGHLHLSRTQIDWFVYERGELVGH
                     SLEVLDTNDIQGFENAMNLMIDDVQTRLQSAFELVVVSGECTGDHRSRMDWLRTQLQA
                     ELIQVFDLAGLQPPSAAMPATSLAVSEMVPLLGAVYAYLNDHGADFRGMVRSRPVAHQ
                     PPGLIDQLKGLVAGAVINVTPGGTRRVEYLGVVLGLVAIAVLVGGQYTWLVRREAQLQ
                     LEQSVQTRRAEANQALKQEAEQYRQYLDALNHRIEVVNKMREAQGMNYQISNDVSWQV
                     PSGLVLTTLKVQGRNVTISGYVTPTTVTQAQAEAGQPLVLDVVDPVADFVRNLEGAGR
                     YTNVQSNYDLQPQEEKSDFTITCQYQGNVFTDPIEPQPVPELVVKATENTTAAPKTAT
                     PAKSGK"
     gene            complement(3425..4084)
                     /locus_tag="PKZ53_02835"
     CDS             complement(3425..4084)
                     /locus_tag="PKZ53_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ39398.1"
                     /translation="MGLRSPAQLEETQLMMPQLLRNLRRMMAQKYAGSGYSLVVHGEE
                     VGLAAAQIARILHRAHPECEQKAQLAYLAGALHDYGKIVVPDEIRLSRQVYSESERQV
                     MEYHPIYGKLLLESVGCHEEIVAAAYYHHLRYGGGGYPNCGLTGDQIPLIARMVTVAD
                     HFIARIEHRPHRPGQCPIEVWADMQRQRHILFDPRVLDAFEETELLQSALGTHPLRIF
                     A"
     gene            4739..>5054
                     /locus_tag="PKZ53_02840"
     CDS             4739..>5054
                     /locus_tag="PKZ53_02840"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ39399.1"
                     /translation="MTKLLEGKTALVTGGSRGLGRAICEVFAREGCNVAFNYHSQPEA
                     AQTVIDNIEHLGQRALAFQVSVTDRAAVKQMVRQVIEQYGRIDILVNNAAINRADMFL
                     TMT"
BASE COUNT         1280 a         1304 c         1233 g         1237 t
ORIGIN      
        1 gccagattga tcattgattg atacaccgca aaatcaagcg ggatttcagc ctgccgccgc
       61 cactcaccat ccagaaacaa ttcgtgctgg atggcacgcc gatcccagtc atagaaaatc
      121 cgaacgtccg aggcttcctg acgaaaggca gacccggcaa tatcatagag cacctgtctg
      181 gtgtgactaa aactttgggg ggcttcagtt tttggccgac caggtgtgga aaccgcctgg
      241 gttgcccact cacgtttgac agcctgcaca tagcgccgcg tgtcacgctg ggtaaatcct
      301 tcatatcggg tttcggcata gagtcgtttg atttcgcggt caatcacctc gcggggaacc
      361 cgtcgaggct cacagcggaa ttcacccgcc aggcgttcat caacctgtgc cgcaacagca
      421 atcgccgtgc gaatatcagc ccgattgagg agaaattcca gcgtctgtcg cccgtttcca
      481 tcggcataga gagcatgaat ggtggtgcca accaggcatt caatcagaat ggcgtggcgt
      541 tcagccgttt cacgcgggat cacggttgcc acggcggcct ggaatcgaag ttccatggcc
      601 tttggatagg aattggaaat ccctttgagc acatcttcaa ctgcatgacg actggttttg
      661 agcaccaacc gggacacttc aggcagtgtt aatcgggcag cacgtgccag actgcgttgg
      721 aagtcaagtt ggtgcatcac ctgagtcaaa taggcggatc cggcatcgtc aaccacggcg
      781 gcttttaatt cctgtgacgt gactcccaaa accagaactt tgtgttccag agcttcattg
      841 attgggatga ctccggccag ttgcggattt gatcgcaaat ttggacgggt cataagcgaa
      901 agtgatgatc tgaacatgac ggttggtcct ctatttgaat caggttatgg aaacaaaaag
      961 agcgaaaaag gaaatggaaa gaaaaacatc aaatcaatca gtttgtgctc gatctggtct
     1021 cgcagggttt ccatttcgcg ctccattgca gggttcaggg ttgtgggttc agggtcttcg
     1081 aatttatgtc tcttatgtcc cttatgtcct ggatgtcttt ttttgaaacc ccgcggcccc
     1141 aaacccacta ccctttggcc agaattgtat tggtcgcttc ggcaacttct ttggtttgaa
     1201 catcaaccgg agcaaatccc ttgagcgtca tggtggttgt tccatcagta cttcgaactt
     1261 cctgctctga aaagaccagc aaccgtgggt gattgcccat ccattccaaa aatttaagat
     1321 ggttgaggaa cgaaccccgc aactggatca ccagcctcga ttcattgatt cctggtttat
     1381 atggtgcgtg catatcatct ttgcgctcaa tgacaagatg ggcagcccga gcctggtttt
     1441 ttacctgttc aaataactcg ccaagcccat ccacggacgg caaatatttt tcaacctgac
     1501 gatactgatc acgggtggct tcgactttgg caaccagttg atcgtgttct tgccgatagc
     1561 gatccgtaat atcattttcc tttgtttgtc tggcaatcac gtcttgctgc gtattcaatt
     1621 ttgattgcca ttcagaaaac caaaactggt cggcaacaac ccaaccactg gcagccatca
     1681 acacagcaat gattccttgc gttacaattc gtcggaccag catgatccat tcctccgctg
     1741 ctataaataa tgaatgataa atgacgaagg atgaatgacg aaggcatttc tcatcaaaga
     1801 gatatggtga gatcaaatga agtttgaaat gccataactc atcattcatt tctcagccga
     1861 ttcttttatt tcccagattt ggctggcgtc gccgtcttcg gtgcggcggt cgtattttcc
     1921 gtggctttca caaccagttc gggaaccggc tggggttcaa ttgggtcggt aaaaacattg
     1981 ccctgatact ggcaggtaat ggtgaaatca cttttttctt cttggggttg caggtcgtaa
     2041 tttgattgca cattggtata gcgaccggca ccttccagat tgcgaacaaa gtctgcaact
     2101 ggatccacca catcaagaac gagcggttga ccagcttctg cctgggcttg agtcacggtc
     2161 gtgggtgtta catatccact gatcgttaca ttgcgtccct ggactttcag cgttgtcagc
     2221 accagcccgg atggcacctg ccagctcacg tcatttgaaa tctgatagtt catcccctgg
     2281 gcttcccgca tcttattcac cacttcaatg cggtggttta aggcatcaag atactgccgg
     2341 tactgttcag cttcttgctt gagtgcctga ttggcttcgg cacgtcgggt ttgaaccgat
     2401 tgttcaagtt gcaactgagc ctcacggcga accagccagg tatattgtcc gcctaccagc
     2461 accgcaatcg ccaccagtcc caaaacaacc ccaagatact caactcgacg ggtaccacca
     2521 ggtgttacgt tgatcactgc gccagccacc agacctttga gttgatcaat caatcctggt
     2581 ggctgatgcg caactggccg cgaacgcacc attccccgaa aatcagcgcc gtggtcgttg
     2641 agataagcat acacggcccc aagcaacggc accatctcgg aaacagccag actggtggcc
     2701 ggcattgcgg cggaaggggg ttgcaatccg gcaagatcaa agacctgaat cagttcggcc
     2761 tgcagttgtg tccgcagcca gtccatccgg gaccgatggt cacccgtaca ttcgccagaa
     2821 acaaccacca gttcaaatgc cgattgcaat cgggtttgga cgtcatcaat catcaggttc
     2881 atcgcatttt caaatccctg aatatcgttg gtgtccaaca cttcaagact atgcccaacc
     2941 agttctccgc gctcatagac aaaccagtca atttgcgtcc tggaaagatg aaggtgcccg
     3001 gctcgaacgg cctgctcagc ttccctgcca tacacggtgg taaaggcttc agccaggcag
     3061 gcggcttcgc acaaaaccga tccgaccggg aggtgctcga caatcatcgg atccagatag
     3121 gccagcatca aggtggcgtc aaaatcacat cgggcagtta ccagatttga tagcgccacc
     3181 gaactagcca gatcggtcat aaatgcctca ccaggacgga cttcaactgg gctgggatag
     3241 atggtcaata cctggatatc actggcaatt gattggagcg tagcgacggc atcagcttgt
     3301 gatgcaagct gttccagatg aaccagcgtt ggaccgcttt ttccatcggt tttgacaata
     3361 ccaagggtaa ccccggctga ttgatagcca aagcgaatga ctcctaacat gatagccgtt
     3421 tcctctatgc aaaaattcga agtggatggg ttccaagagc tgactgcaac aattcagtct
     3481 cctcaaaagc gtccaggact cgtggatcaa aaaggatatg acgctgacgc tgcatatcgg
     3541 cccagacttc tatcgggcac tgacctgggc gatgcggacg atgctcaatc cgggcaataa
     3601 aatgatccgc cactgtaacc atgcgcgcga tcaggggaat ctgatcccct gtcaacccgc
     3661 aatttggata tccgccaccg ccatatcgaa ggtggtggta ataggctgct gcgacaatct
     3721 cttcgtggca gccgactgat tcaaggagca gtttgccgta aatcggatgg tattccatta
     3781 cctggcgttc cgactctgaa tacacctgcc gcgataaccg aatttcatca ggaaccacaa
     3841 ttttgccgta atcgtgcaat gctccggcca gatatgccag ttgcgctttt tgctcacact
     3901 caggatgagc acggtgcagg atacgggcaa tttgtgcggc ggccaggccg acttcttcgc
     3961 catgaaccac cagtgagtac cctgacccgg catatttttg agccatcatc cggcgcaagt
     4021 tgcgcaacag ttgcggcatc atcagttgag tttcttcaag ttgtgcaggg gaacggaggc
     4081 ccatatggca atacctcgca gatcgagttt caatgttacc caccgattga ttctgatggt
     4141 gcggatgcca gagtaagggc aaccctcgtg ccagaaagtt tatccagaaa ttcaccctaa
     4201 aaatcctgcc tgagcccgga attcatcgct ttgaattgaa tctcctgacc tgataatggc
     4261 ttgaaccagt ccagatgata ccatccctgt gaatggttta atttctctta aaacactgaa
     4321 aataaagata tttcatcttg atgtgcggct gagcgatcca gggttggggg ttcagaaccg
     4381 tgaaaaacag gtctgcattc aattgattca attaatttag attcagcaaa ccgaggaaag
     4441 ttaaattaaa ctggtctagc aaaacgaaac acaatccaat ttcaattaaa ttgatcaatt
     4501 gatcgtgcca ccatcgcctg gattcttccc aggaatttca atgggcgatg gaatcctcgc
     4561 ctgagaaaat ggtcaaatga ttcaacgaaa tgagcttagt gaacgactgg cgacgagcga
     4621 ctgacgacaa actggtataa gatattccat ctcctgtctg gacatcacgg gaggttgccc
     4681 atacaatcgg cgattcaaat accctcaccc gacctatcaa atatcactca cccacgacat
     4741 gacaaaatta cttgaaggaa aaacggcact ggtaaccggc ggttcacgag gactggggcg
     4801 cgcaatctgc gaagtgtttg cccgcgaagg ctgtaatgtg gcgtttaatt accacagcca
     4861 gcctgaggcc gctcaaaccg taattgataa tatcgagcat ctgggtcagc gcgcactggc
     4921 ttttcaggtc tcggtcacag atcgcgcagc agtgaaacaa atggtgcggc aagttattga
     4981 acaatatggc cgaattgata ttttggtgaa taacgccgcg attaaccggg cagatatgtt
     5041 tttgacgatg acgg
//
LOCUS       DAOQDO010000111         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10742_length_5044_cov_7.914412, whole genome shotgun sequence.
ACCESSION   DAOQDO010000111 DAOQDO010000000
VERSION     DAOQDO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10742_length_5044_cov_7.914412"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKZ53_02845"
     CDS             complement(<1..640)
                     /locus_tag="PKZ53_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ39400.1"
                     /translation="MTFTAIFRHCPLRIFAVTAMLLLSGFGSAPGLAQEPGVAPAPPS
                     PSKESPSPSKSPQDPPKNSDEPIIRAQTNLVTLTVTVTDPSGRFVTGLEQDHFEIFDN
                     KVLQKIEFFSDDDAPVSVGIIFDLSGSMRGRIHRAQEALRKFLDACHEEDEFFLVGFN
                     SVAKLLSDFTPDGEKLANMLTLSETKGQTALYDACYIGVEKVREGRHAGCRRF"
     gene            1150..3876
                     /locus_tag="PKZ53_02850"
     CDS             1150..3876
                     /locus_tag="PKZ53_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ39401.1"
                     /translation="MARTRPSTKTPRFLLVLLTFCALFYTGGRAYFASESFFLHPRIG
                     FSADYDLQSRQFAVVDVRPGSAAEKIGLVKGDRVLSIGGEPVQSYAHFTQLIRHAQRM
                     HKLEISIQKPDGRILAPTAELPVPQVELFSYPFFYYFLSLIISTICSVIGLAICWLRW
                     EDRTAQLGGLFFISIGVLFSFNLQTLPAWLLPFFVLYINIAYWFSGYWCFAFFSEFPG
                     VSLFASHWSWLRRLYLATLLAVTPIGVVSLLTYTYSFEAAAQYGTLTYQFIDSFISVL
                     PVVGYFGAVLSGLTQGRLDADQRRRFRVMTLGIILGCGPTLSLMVMSFVRYRTIELRD
                     YPQWLVATCLALFTLFPLSFAYTIVKHQVLGIQQILRRGLQHAFVSYGYLLVEVVILF
                     LLVMYPATIIARKVFLAFHYSPPDMVRHGISYFLGALGLLAMWKINPHLQSIIDRAFF
                     RNTYQAQQVLSDLGRSMRQLTQVEEVVQCVAERIGATLLVNRVVVFLQPHFLNVNHDS
                     PGNPRLRAYVCRFDVSSQGQIDAELSLPGGSLLMKQLAKSAGPLEVYYNTADAWTERL
                     IQQDRGNPFSNVVEYYQQERDLLIRINASLLIPLATKDGLLGCISLGTRLSEEPYTPE
                     DKDLLMAMAEATALRLENSLLIRRMAEEASLKRELEIARTMQQSLLPSQDPILPGFEC
                     AGYSVSANDVGGDYYEYFVLNEVTFAIAVGDVTGHGVSSGLLMALAKGGLLNQISMNA
                     TPSAVMYAMNNLICAAGSRKNLMTFAYGVIDSRTLTIELSNAGHPSPYLYRAATRTVE
                     ALESSAYPLGVRRNCEFPVLHQTLKAGDAIVFYSDGIIEAQNAAGTLFGYERLEQAIL
                     ENGDCSAEALRDAILDDSRSFLEVTRTFSGNSARLIEDDITLVVLKARVNAP"
     gene            complement(3873..4985)
                     /locus_tag="PKZ53_02855"
     CDS             complement(3873..4985)
                     /locus_tag="PKZ53_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNJ39402.1"
                     /translation="MLSLLDHFSDLPDPRVDRTKLHLLIDIVTIALCAVICGAETYEE
                     METFGESKHEWLATFLELPNGIPSHDTFRRVLMRLNPKEFESRFLTWVSCVADLTQGE
                     IVSIDGKTLRGSRSKSNSTIHMVSAWAAANRLVLGQVKTEDHSNEITAIPELLSVLML
                     KGCIVTIDAMGCQTEIARQIHEQDADYVLTLKANQPTTHQAVISFFDQARLAPGPNPA
                     LSFFQTVDNDHGRLETRRCFAAPASAALDPELLARWPGLTSVVKITATRDIDGDRSTE
                     SRYFLSTLPPDAQHLLSVVRTHWQIENCLHWVLDVAFREDFSRVRSGHAPHNFAILRH
                     IALNLLKQETSCKNGIKSKRLKAGWDHQYLLKVINS"
BASE COUNT         1112 a         1215 c         1385 g         1332 t
ORIGIN      
        1 cgaatcggcg gcatccagca tggcggcctt cgcgaacttt ttcaacgccg atgtagcagg
       61 catcatagag ggctgtttgg cctttggttt ctgacagtgt cagcatgttg gccagctttt
      121 cgccatccgg agtaaaatca gacaggagtt tggccacaga attaaacccg accagaaaga
      181 attcgtcctc ttcgtggcag gcatcaagaa atttgcggag cgcttcttga gcacggtgaa
      241 tccgaccacg catgctgcct gacaaatcaa aaataatccc gacggacacc ggggcatcat
      301 cgtcactgaa aaattcgatt ttttgcaata ccttattgtc aaagatttca aaatgatcct
      361 gttccaaccc ggtcacaaac cgcccagagg ggtcagtcac ggtgacggtg agggttacca
      421 gattggtttg ggcgcggatg attggctcat cagagttttt gggagggtcc tgtgggcttt
      481 ttgaaggaga aggagattct ttagaagggg aaggcggtgc gggggcaacc cctggttcct
      541 gggccaaccc tggggctgat ccaaatcctg acagcagcaa catggccgtc accgcaaaaa
      601 ttctcaacgg gcaatgcctg aaaatggctg taaacgtcat agcattgatt ctccttgatc
      661 caaagttccg cctggatgag ccaaaaaaag atgatgattg ctgcccgctg gacgtggctt
      721 tagccagaga acatccaggt tgcagattca aaacagtggt gaatatctgg tatggtttga
      781 gtgtttggcg gaaagagccc tcgtgactat agcaggttcc tggtgaaatt gggattgcga
      841 acaccaaaaa ccaaatttct gaaacgcaaa accgatgtga gcgttcaaaa agtttgtcaa
      901 tttttctgcc atcccagcat gccaatcctg tttccaaact gtttttctga tgaccaatct
      961 ggctgaacag gccagtcggt ccagattttg aagctcaggt gtaatccgtg gggtgggcgg
     1021 cacatattcc gccaactctg caaaaatcac ctttttccct tcaaaaattt ggtaatctcc
     1081 tcttgacctc agggttgact ctgtcattgt tattactgat ttatttctct tcagggttat
     1141 tcgagttcca tggccaggac acgaccaagc acgaaaacac ctcgctttct actggtactt
     1201 ctgacgtttt gcgcgttgtt ttataccggt ggacgggcct attttgcttc cgagagcttt
     1261 tttttacacc cacggatcgg attttcagcc gattatgatc tgcagtcacg tcaatttgca
     1321 gtggttgacg ttcggcctgg cagcgcggct gagaagatag ggctggtgaa aggagatcgg
     1381 gtcctctcaa ttggcggaga acccgtccag agttatgccc actttaccca actcatccgg
     1441 catgcccagc gcatgcacaa gcttgagatc tcgatccaga aaccagacgg aagaatcctt
     1501 gccccgacgg ctgagttgcc tgtgcctcag gtcgaactct tttcctatcc ctttttctat
     1561 tattttctct cattaattat ttcaacgatc tgtagcgtca ttggacttgc gatttgctgg
     1621 cttcgatggg aagaccggac ggctcaactc ggtggactct tttttatttc gataggcgta
     1681 ctgtttagct ttaatttaca aaccctgccg gcctggctcc tgccgttttt tgtgctctat
     1741 atcaatattg cctactggtt ttcaggctac tggtgttttg cctttttctc tgaatttcct
     1801 ggggtatcgc tgtttgcttc acactggtca tggctgcggc gactctatct tgccacgctg
     1861 ctggccgtca caccgattgg ggtggtgtcg ctgctgacct atacctattc gtttgaagct
     1921 gccgcccagt acggcactct gacctatcag tttattgata gttttatttc agttttgccg
     1981 gtggttggtt attttggcgc tgttttgtca gggttgaccc agggaaggct tgatgccgac
     2041 cagcgccgtc ggtttcgggt catgacgctg ggaattattc ttggttgcgg cccgactctg
     2101 agtttgatgg tgatgagctt tgttcggtat cgaaccattg aactccgtga ctacccccag
     2161 tggctggttg cgacctgcct ggccttgttc acactctttc cactgtcgtt tgcctacaca
     2221 attgtcaaac accaggtact tggcattcaa cagattcttc gccggggtct gcagcatgcg
     2281 tttgtgtcct atggctattt gctggttgaa gtcgtcattc tgtttttgct ggtgatgtac
     2341 ccggccacca tcattgcccg caaggtcttt ctggcttttc actactctcc gccagatatg
     2401 gttcgccacg gaatatccta ttttctcggc gccctgggat tgcttgcgat gtggaaaatc
     2461 aacccccatt tgcagtcaat cattgaccgg gcatttttcc gcaataccta tcaggcccag
     2521 caggtactga gcgacctggg gcgttcgatg cgccagttga ctcaggtgga agaagttgtg
     2581 cagtgcgtcg cagagcggat tggcgccacc ctgctcgtta atcgggtggt ggtgtttttg
     2641 caaccccatt ttttgaatgt caatcacgac tcgccgggga atccacgctt acgggcctat
     2701 gtctgtcgtt ttgatgtttc cagccagggc cagattgatg ctgaattgtc actgccaggt
     2761 gggtcgctcc tgatgaagca gcttgccaaa tcagccggac cgcttgaggt ttactacaat
     2821 acggctgatg cctggaccga acgattgatc cagcaggatc gcggtaatcc attttcgaat
     2881 gtagtcgaat actatcaaca ggaacgtgat ttgctgatcc gcatcaacgc cagtctgttg
     2941 atcccgctgg caaccaaaga tggtttgtta ggctgtatca gcctcggtac caggctctct
     3001 gaagaaccct ataccccgga agataaagac ttgttgatgg ccatggctga ggccactgcc
     3061 ctgcgtctgg aaaactcgtt gctcattcgt cgcatggccg aggaagccag cctcaaacgc
     3121 gaactagaaa ttgcccgaac catgcagcaa agcctgctcc caagccagga cccgattttg
     3181 cctgggtttg aatgtgccgg gtattcggtg tcagccaatg atgtgggagg ggattactat
     3241 gaatactttg tgcttaacga ggtgacgttt gcgattgctg tcggggatgt cacgggccat
     3301 ggtgtgtcct caggattact gatggctctg gcaaaaggtg ggttactcaa tcaaatcagc
     3361 atgaatgcca cccccagtgc ggtgatgtat gccatgaata atttgatttg tgcggctgga
     3421 agccggaaaa acctgatgac gtttgcctat ggtgtcattg acagtcgaac attaaccatt
     3481 gaattgtcaa atgcgggtca cccgtcccca tatttgtatc gggcggcaac gagaactgtc
     3541 gaagccctgg aaagttcggc gtatccgtta ggtgttcgac gaaattgtga atttccagtg
     3601 ttgcaccaaa cgctcaaggc tggcgacgcc attgtttttt acagtgatgg catcattgaa
     3661 gcccaaaatg ccgcgggtac cttatttgga tatgagcgcc tggagcaggc cattcttgaa
     3721 aatggcgatt gcagtgccga agccttgcgg gatgcgattc tggatgattc gcgttcgttt
     3781 ttggaagtca cccggacgtt ttcagggaac tctgctcgcc tgattgaaga cgatattacg
     3841 ctggtggttt taaaagcaag ggtaaacgca ccttaactat ttatgacttt taggagatac
     3901 tggtgatccc accccgcctt caagcgtttg gatttgatgc cgtttttgca ggaagtctcc
     3961 tgtttgagca aattgagggc gatgtgccga agaatggcaa aattgtgagg agcgtgaccg
     4021 gaacggacgc gggagaaatc ttcgcggaag gcgacgtcga gaacccaatg gaggcaattt
     4081 tcaatctgcc agtgagtgcg cacgaccgag aggaggtgct gagcgtcagg cggcagggtg
     4141 gaaagaaaat agcgggattc ggtcgagcgg tcaccgtcaa tgtcacgggt ggcggtaatt
     4201 ttgacgaccg aggtgaggcc aggccagcgg gcgaggagtt cgggatcaag ggcggcactg
     4261 gctggggcag caaaacagcg acgggtttca agccgaccgt ggtcgttatc gacggtttgg
     4321 aaaaaagaaa gggctggatt tgggccggga gcgagtcggg cctggtcaaa aaaggagatg
     4381 accgcctgat gggtcgtggg ctggttggct ttgagggtca ggacatagtc ggcatcttgc
     4441 tcgtgaatct ggcgtgcaat ttcggtctgg cagcccatgg catcaatggt cacaatgcag
     4501 cccttgagca tcaggaccga gagcagttcc gggatggcgg tgatttcgtt ggaatggtcc
     4561 tcggttttga cctgacccag gaccaggcgg ttggcggcgg cccaggcgct gaccatgtga
     4621 atggtggagt tggacttgct gcgcgaccca cgcagggttt ttccgtcaat gctgacgatt
     4681 tcgccctgag tgagatccgc cacacagctc acccaggtca gaaaccgcga ctcaaactct
     4741 tttggattga gccgcatcag cacccgtcga aaggtatcgt gggacgggat tccgttgggt
     4801 aactcaagga aggtcgccaa ccactcgtgt ttcgactcgc cgaaggtttc catttcctcg
     4861 taggtctcgg ccccgcagat cacggcgcac agcgcaatgg tcacgatatc gattaacagg
     4921 tgaagtttcg tccgatccac acgtggatcg gggaggtctg aaaaatggtc gagtaaggaa
     4981 agcatgcgtt gactccaggt tgatcaggta aaaaacctgg tatgtatcac atttgttttg
     5041 tcaa
//
LOCUS       DAOQDO010000112         5028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10799_length_5028_cov_3.016690, whole genome shotgun sequence.
ACCESSION   DAOQDO010000112 DAOQDO010000000
VERSION     DAOQDO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10799_length_5028_cov_3.016690"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PKZ53_02860"
     CDS             <1..1496
                     /locus_tag="PKZ53_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39403.1"
                     /translation="TEPLAEIQPLEEAAAPEPTPTAVLEPATVPEFALAPEPVEAEET
                     AQPSFVVTEPITEVSEPVVEAFTVEPSSVEPEPVLEAAATESVVEAVEVAEPTEISAQ
                     VYEAVTEPSFELPSPVVEQAEPVFELPPPPVETAELVTEAEAVPHTIDEPAVTLESIP
                     VSEPVEEPVVDALVEPEPVVTEELAAPVAEEAAITLSEAPVVEEVPVEAISVAEPEPA
                     AEPDAGLTAGVVAAVGATLLAAPLVAEAVSGGGEVAEAVTEAQPVEAVVAPVAEEIPV
                     EEPALVETVEAAAPAVEEAPAAPVAPVVVAAEVTDISQISPSVIDQLVQRAVAAVPAA
                     PVVTTEVTDISQISPVVVYQLTQRAASAITPAPVAPVVTEVTDISQISPSVIEYIVQR
                     ATPAPTAPVVTEVTDISQISQTVIDQLIQRVAEAAPPVAAAPVVTAEVTDIAQIPQAV
                     VGQIVERVIVELSDRVVREIAWEVVPDLAELLIKQHLESGALKSSSR"
     gene            1632..4373
                     /locus_tag="PKZ53_02865"
     CDS             1632..4373
                     /locus_tag="PKZ53_02865"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNJ39404.1"
                     /translation="MTEQAQPKAQPTEQEIPKVYNPAQIEQNWYPVWENKGYFQPQGN
                     GEPYCIVIPPPNVTGYLHMGHALQHSLMDTLTRWRRMQGLKTLWLPGTDHAGIATQMV
                     VERQLNEQGIRRVDLGREAFEQKVWEWREHSGGTIQKQMRLEGVSVDWSRERFTLDEG
                     LSEAVREVFVRLYDQGLIFRGAYMVNWSPVLNTAVSDLEVEMKEVKGKLYHIKYPIKD
                     SDDYVVVATTRPETMLGDTAVAIHPDDERYQPYHGKHVVLPLVGREIPFITDHLVEMD
                     FGTGVVKVTPAHDPNDFEMGKRHNLEFISVIGKDAKMTAEAGEAFVGLDRYKARAKVI
                     ELLTEQGLLEKIEDYTHKVGHCQRSQVAIEPLISDQWFLDVKDMAQKAIQAVKDGRTQ
                     FVPNNWEKVFFDWLENIRPWCVSRQLWWGHRIPAWYTPEGKIVVARSEAEARERLGNP
                     TVELRQETDVLDTWFSSALWPFSTLGWPNQTEDLKTYYPTTILVTGFDIIFFWVARMM
                     MFGLYFMDDVPFRTVYITGLVRDGMGQKMSKTKGNVVDPLEVFVRYGTDAARFTLVSA
                     VTSGNDIKLQEKKKKDEPSDFPQMEAARNFANKIWNASRFVLMNLEVGTEVEADNSDS
                     MVPHSLPDRWILSELNQTAIDVNAALENNRFHDAAQSLYHFFWDDFCDWYIELSKPLV
                     TATQVTPEVKAARQRIVTVLEQSLRLLHPIMPFITEELWQRLKIWRDANGETDLPESI
                     CIAPFPAGDRSLIDEEAHQDMDVLTGIITRVRNIRSEMNIPPSIYFTLNLATPNEHLA
                     DMVREYEWAIKRLARVETLNVSPELPATGLAAKAVLAEVELSIPLEGVIDLEKEKTRL
                     TNEVTRLQKQLTGLLAALNAPDFASKAPEHIVAEKNAQREELETKLAKLNEVLAMMN"
     gene            4577..>5028
                     /locus_tag="PKZ53_02870"
     CDS             4577..>5028
                     /locus_tag="PKZ53_02870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098605.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNJ39405.1"
                     /translation="MRLDYNIIQHLVASYLEEDLGRGDVTTSTVVPVDAEARGRFLAK
                     QSLVLAGLEVAECVFQWLDPELQLEAFYFDGDTIPTGTEVARIAGPAHILLAGERVAL
                     NLLQRMSGIATLTRTYVKAVEGTPASICDTRKTAPGLRVFDKYAVRIGG"
BASE COUNT         1262 a         1325 c         1332 g         1109 t
ORIGIN      
        1 gaaccgaacc gctggctgaa atccagccgc tggaagaggc cgctgctcca gagccaacgc
       61 caacagcggt gcttgaacca gccaccgtac cggaatttgc cctcgcccca gaaccagttg
      121 aagctgaaga aacagcccaa ccatcatttg tggtgacaga gccgatcact gaggtttctg
      181 aaccggttgt cgaagccttc acggttgagc catcatcggt tgaaccagaa ccagtgcttg
      241 aggctgccgc cactgagtca gtcgttgaag cggtggaagt tgccgaaccg actgaaattt
      301 cagctcaggt gtatgaagcg gttaccgaac cttcctttga gctgccttca cccgtcgttg
      361 aacaggccga accggttttt gaattaccgc ctccgccagt tgagacggct gaactggtga
      421 ctgaagctga agcggtgccg cataccattg acgaaccagc cgtgaccctg gaatcaatac
      481 cggtgagcga accggtggaa gaaccagtcg tggatgccct ggttgagcca gaacctgttg
      541 ttactgaaga gcttgccgcc ccagttgctg aagaagccgc gattacactg agtgaagccc
      601 cggttgtgga agaagttccg gtcgaggcca tttcggtggc tgaaccagag cctgcggctg
      661 aacctgacgc tggcctcacc gctggagtgg tggctgcggt tggagcaacc ctcctggccg
      721 cgccgctggt ggccgaagcg gtttccggcg gtggtgaggt cgctgaagct gtgacagaag
      781 cccaaccagt ggaagcggtt gtcgccccgg tcgccgaaga aatcccggtc gaagaaccgg
      841 ccctggttga aacggttgaa gcggctgccc cagccgttga ggaagctccg gctgccccag
      901 ttgccccggt ggttgtggcg gctgaagtga ccgacatcag ccagatttca ccatcggtga
      961 ttgatcaact ggtgcaacgt gcggttgcag ccgtgccggc agccccagtt gtgacgactg
     1021 aagtcactga tatcagccag atttccccag tcgtcgttta tcaacttacg cagcgtgctg
     1081 cctcagccat cacacctgcc cccgttgccc cggtggtcac tgaagtcacg gatatcagtc
     1141 agatttcacc atccgtgatc gaatacattg tgcagcgtgc cacccctgct cctactgccc
     1201 cggtggtcac tgaagtcacc gatatcagcc agatttcgca aactgtcatt gatcaactga
     1261 tccagcgtgt ggctgaagct gcgccaccag ttgccgcggc gccggttgta acggctgaag
     1321 taacggacat tgcccagatt ccgcaagctg tcgtcggaca gattgtagaa cgcgtcatcg
     1381 tagaactctc tgatcgagtg gtccgcgaaa ttgcgtggga agttgtccca gacctggccg
     1441 aattgctcat taagcaacat cttgaaagcg gtgctctgaa aagttcatcc cggtaaggtt
     1501 gccaaatagt gacgtaattt gttgtaagga aaagcagcgg atcaacccag taccagttga
     1561 tccgctttgc ttttttttga gcggcgactg ggcaaacgta ccaaccctca aacccaaaaa
     1621 ttttcaaatc tatgaccgaa caagcacaac caaaagcaca accaaccgaa caggaaattc
     1681 caaaagttta taacccggcc cagattgaac agaactggta cccagtctgg gaaaacaaag
     1741 ggtattttca gccccagggc aatggcgaac cctattgtat tgtgattcct ccaccaaacg
     1801 tcaccgggta tctgcacatg ggtcacgcgt tgcaacattc gctgatggat actctgaccc
     1861 gctggcgacg gatgcagggc ctcaaaaccc tgtggctgcc cggcaccgac cacgccggaa
     1921 ttgccaccca aatggtcgtt gagcgccagc tcaatgaaca gggaattcgt cgcgtggacc
     1981 ttggccgcga agcctttgaa caaaaagtgt gggaatggcg cgaacattcc ggcggcacaa
     2041 tccagaaaca aatgcgtctc gaaggggtca gcgttgactg gtcacgcgaa cgcttcacgc
     2101 ttgatgaagg cttgagcgaa gctgtgcgcg aagtatttgt ccgcctttat gaccagggcc
     2161 tgattttccg gggtgcctat atggtcaact ggtcgccggt gcttaacact gccgtttctg
     2221 accttgaagt cgaaatgaaa gaagtcaagg gcaagctcta tcacatcaaa tatccgatca
     2281 aagacagcga tgactatgtg gttgtcgcca cgactcgacc ggaaacaatg ctgggtgaca
     2341 ccgccgtggc cattcacccg gatgatgaac gctatcaacc atatcacggc aaacacgtgg
     2401 tactgccgct ggttggacgt gaaattccat ttatcaccga tcatctggtt gaaatggact
     2461 ttggaaccgg ggtggtcaaa gtgaccccgg cccacgaccc gaacgacttt gaaatgggca
     2521 agcggcacaa cctggaattc atctcggtaa ttggcaaaga cgccaaaatg acggctgagg
     2581 ccggagaagc atttgtcgga ctggatcgct acaaagctcg cgccaaagtg attgaactgc
     2641 tgaccgagca gggcttgctg gaaaaaatcg aggactatac ccacaaagtt ggtcactgtc
     2701 agcggtcaca ggtcgcaatt gaaccgctca tttccgacca gtggtttttg gatgtgaagg
     2761 acatggccca gaaagccatc caggccgtca aagacggacg cacccagttt gtgcccaaca
     2821 actgggaaaa agtgttcttt gactggcttg aaaacattcg tccgtggtgt gtgtcgcgcc
     2881 aactctggtg gggacatcgg attccagcct ggtacacccc ggaaggaaaa attgtggtgg
     2941 cacgttccga ggccgaagcc cgcgagcggt tgggtaatcc gacggttgag ttacggcagg
     3001 aaacagatgt gcttgatacc tggttttcat cggcactctg gccgttttcg acgcttggct
     3061 ggccaaacca gaccgaagac ctgaaaacct attatccaac cacgattttg gtcacgggct
     3121 ttgacattat ctttttctgg gtggctcgaa tgatgatgtt tgggctctac tttatggacg
     3181 atgtgccctt ccgaaccgtc tatatcacgg gcctggtgcg tgacgggatg gggcagaaaa
     3241 tgtccaaaac caaaggaaat gtggtggatc cgctcgaagt gtttgttcgc tatggaacgg
     3301 atgctgcacg ttttaccctg gtttccgctg tgaccagtgg caatgacatt aagctccagg
     3361 agaaaaagaa aaaagacgaa cccagcgact ttccccaaat ggaagctgcc cggaattttg
     3421 ccaacaaaat ctggaatgcc agccgctttg tgttgatgaa cctggaagtt ggaaccgaag
     3481 tcgaagccga taacagtgac tcaatggtcc cgcattcgct tccagatcgc tggattctct
     3541 cggaactcaa tcaaaccgca attgatgtca atgcggcctt agagaataat cggttccacg
     3601 atgccgcaca gtcactctat catttcttct gggatgactt ttgcgactgg tatattgaac
     3661 tctcaaaacc gctggtgacg gccacccagg tgactcctga agtcaaagcc gcccggcaac
     3721 ggattgtgac tgtgctggag caatcgctca ggttattgca cccgattatg cccttcatta
     3781 ctgaagagtt atggcagcgg ttgaaaatct ggcgtgatgc caatggtgag actgatctac
     3841 cagaaagcat ttgtattgct cccttcccag ccggagaccg gtcgctcatt gatgaagaag
     3901 ctcaccagga tatggatgta ctgaccggaa ttatcacccg tgtgcgcaac attcgttcag
     3961 agatgaacat tccgccatca atttacttca ccttgaacct ggccacacca aacgaacatc
     4021 tggcggatat ggtccgggaa tatgagtggg caatcaagcg gctggccaga gttgaaacct
     4081 tgaacgtgtc acctgaactg ccagccactg gattggctgc aaaagccgtc ctggctgaag
     4141 tcgaactttc gattccgctg gaaggtgtga ttgaccttga gaaagaaaag acccgtctca
     4201 ccaacgaagt gacccggctc cagaagcagt taactggatt actggcggcc ctcaatgcgc
     4261 cagactttgc ttcaaaggca ccggaacata tcgtggctga gaaaaacgcc caacgtgaag
     4321 aactcgaaac caaactggcc aaactcaacg aagttctggc catgatgaac taaaggaatg
     4381 aagaattgag aatagaaagt cgctataacc catcaactaa aaaaaccctg gaatacacag
     4441 cacacacgga aaataaattc aaattattca attggttctg atatacaacc gtggacgaga
     4501 cagggaacta tttatctgaa aggcgataga tttcttcatt ctcaattctt cattcttcat
     4561 tctgtatttc aaacttatgc gacttgatta caacattatt cagcatttag tagcttcgta
     4621 ccttgaagaa gacctgggcc gaggcgatgt cacgaccagt accgtcgtcc cggtcgacgc
     4681 tgaggctcgg gggcgatttt tggccaaaca gtcacttgtt ctggctggac tcgaagtcgc
     4741 cgaatgtgtc tttcagtggc tcgacccaga acttcagctt gaagcctttt attttgatgg
     4801 agataccatc ccaactggaa ccgaagtggc ccggattgcc ggcccggcac acattctact
     4861 ggctggcgaa cgagtggcct taaacctgct ccaacggatg tcagggattg ccaccttgac
     4921 ccgaacctat gtcaaagctg ttgaagggac accggccagc atctgtgata cccgcaaaac
     4981 agcaccgggc ctgcgggtct ttgacaagta tgccgttcga atcggcgg
//
LOCUS       DAOQDO010000113         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10834_length_5019_cov_2.765915, whole genome shotgun sequence.
ACCESSION   DAOQDO010000113 DAOQDO010000000
VERSION     DAOQDO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_5019_cov_2.765915"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKZ53_02875"
     CDS             <1..246
                     /locus_tag="PKZ53_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39406.1"
                     /translation="PVLMDGGSVTAPHFAFYTPEGQWEVENHGVAPDVEIELDPKLWR
                     QGRDLQLEKAVEWLLAELKKNPPKEYRRPAYPNYHKK"
     gene            550..2868
                     /locus_tag="PKZ53_02880"
     CDS             550..2868
                     /locus_tag="PKZ53_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ39407.1"
                     /translation="MCNYRFSVPGLLVLIILSLCPTVLAQDQELRTLRQTTEKKPQNL
                     GFEDGTLGQLPAGWRRVPAQYPYDAKLVNDRVKQGNQAAYLFPKPGVTPQSYEFGNLM
                     QTVDATKYRNKQVRFRAALRVKTESNESEGRLWIRVDRPHSVVGFFDTTLDDAVYQDQ
                     DWQYIEVIAEVDDDAETLNFGILSIGDAQVWMDDASLEVVGDIISLKEPARPLTEQGR
                     RNVMAFTRMLGYVRHFHPSDQAAEVDWVKFAVHGIRDIEKVATDAELIRALNDLFSPI
                     APTVQVFAEKKPPALHPALFPPTGTTGSISVIRWFHRGFQGSEPRSAYSNQRRSQPYR
                     NNTIPDGYGDPAKPFEANLGGGIQCRIPSNLFRDRDGTFPHVEKRTEFPTIEKFTGND
                     RATRLADVALAWNILQHFYPYFDIANVNWMEVLPKALNLAANDKNEAVFEKTLQWLIA
                     QLNDGHGNAVDAVYPTGAIPLSWTWAENQLVVTSLAVSDIPNLKPGDVVVEVNGKPTA
                     KVIEKLEKYISGATPQWKRFRSVDALRFGDAGDTATLKLRSVEGDLKTVKIEFSSEFL
                     YYAEPRPEKYQELSPGIWYFDLERCNDDDFKAILPKLESAKGIIFDGRGYPSVNPIVI
                     QHLITQDVQSAIFAVPIVSTPDHVSMTDFDTRGRWLLNPKSPHLTAKCVFLIDGRAIS
                     YAESFLAIIEAYKLGELVGEPTAGTNGNIVIKDFPRNFRLVWTGMRVTKHDNTPHHGV
                     GVLPTIPLSRTVKGISERRDEQLEKALSILQQ"
     gene            complement(2974..>5019)
                     /locus_tag="PKZ53_02885"
     CDS             complement(2974..>5019)
                     /locus_tag="PKZ53_02885"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HNJ39408.1"
                     /translation="VTGKDPNERFYIVYYPSAFGYDESVPGSSVWRGCTGGGGPEPFV
                     VDLMNYLNQAYDKFLTLPGTPFTALSLPQYAYVSKLKDAAGESAIGGPLSLSNKLENC
                     DYMKGVAGHELAHVFQGQYFTTGKLGYVSAWLGSEIWFIEASANYMAMQTGMSDAQKK
                     TFLTKDGMKNYLVASLDGNDEESYYAAAHFLEWLSNNYGSDFVAKLLTREKNMGGFPM
                     TIHLASELADRGIKDGLTQVFGDYGVYLLTHPEDMDGLVQSIKSRLSGYPYDPGFADQ
                     ITCNGPFLSDTYHYVELTGKVNSLAMVNMILSSKNSGDGLFVMDGTSTATSNTLKSTT
                     FDFIGSSNASYQGKTPIDKDKNYFSGGKPITIKHFGSGQPTSSIEQMLVNANFTASAD
                     IDIRYYVLQPPTVKTVAAGSITWEFEGVGTGRGLIPPEQIAGFHIYDDKGQQINSQMV
                     KLPASGTQQTYSDSKIAAGGKYTVCIVDRHLNKWPEVKEEDTLVVYNLKPFSKSDNSL
                     YGYPASFSCNGNQLAGFGLRIYKDGLIWEGWGPQVAQNVDASVAKSASTVELIRTTTL
                     ASMPGEEIDDGKWKITEFHPNGKVARTIDITFKGYSLYVTETGSTADYKFVADSDDGE
                     FSIDVSNFGAYLKSYGVFLKFHGHVELFDDKGNYLSSNDDDIRTYETLVLNLSRRQQ"
BASE COUNT         1245 a         1278 c         1233 g         1263 t
ORIGIN      
        1 ccggtcctga tggatggcgg ttcggtgacg gcaccgcact ttgcttttta cacgccagaa
       61 ggtcagtggg aagttgaaaa ccacggcgtc gccccggatg ttgaaatcga actggatccc
      121 aaactctggc gccagggccg tgacctgcaa cttgaaaaag cggtcgaatg gcttctggcc
      181 gaactgaaaa agaacccacc gaaagaatac cgccgcccgg catatccaaa ttaccataag
      241 aaatagggtt ccgcgcttcc gggttttcaa acagggcgtg gctcaaccga gccacgcctt
      301 tttctattgc cctcggtcac tctatcacct ggtcacctgt cactttatcc ctgttttctt
      361 gtactgtggt atgtttgaaa aagttgtcgc gcgtcctgat caaataagtt ttttgacatc
      421 tgtgaggaac cagccacaca gggttcgcta agaaaatttt cagcctcaat gtaatggatt
      481 ttcagttttc ctcacttctg gtgttaaaaa gaaaaatcac gctgcttgat ttgagaaagg
      541 tgggttggta tgtgcaatta tcgcttcagt gtaccgggtc ttttggtgtt gataatactg
      601 agtttgtgtc caacggtact ggcacaggat caagaactcc gtaccttgcg ccaaacaact
      661 gaaaaaaaac ctcaaaacct cggatttgaa gacggcacac tggggcaact cccggctggc
      721 tggcggcgcg tcccggccca gtatccgtat gacgccaaac tggtcaatga tcgggtgaag
      781 cagggcaatc aggctgccta tctttttccc aaacccggag ttacacccca gtcatatgaa
      841 tttggaaacc tgatgcaaac ggtggacgcg accaaatacc ggaataagca ggttcgcttt
      901 cgggcggcac tgagggtgaa gacagagagt aatgaatctg aaggccggct ttggattcgg
      961 gttgatcggc cacacagcgt tgtcggcttt tttgacacga ctttggatga tgcggtgtat
     1021 caggatcagg actggcaata tatcgaggtg attgcggagg ttgacgacga cgctgaaacc
     1081 ctcaatttcg gaattctgtc catcggtgac gctcaggtct ggatggatga tgcctcgctg
     1141 gaagtggtgg gtgacattat ctccttgaaa gaaccagccc gtcctttaac ggaacaaggt
     1201 cgaagaaatg tgatggcctt tacccgaatg cttgggtatg tgcgccactt tcacccgagc
     1261 gatcaggcag cagaagttga ttgggtcaag tttgccgttc acggaattcg tgatattgaa
     1321 aaggtggcga ctgacgcgga attgatccgt gcgctcaatg atttgttctc accaattgca
     1381 ccgaccgttc aggtttttgc agagaaaaaa ccgccggcac tccatccagc cttatttcct
     1441 ccaactggaa caactggttc aatttcagtg atacggtggt ttcatcgagg tttccaaggg
     1501 agtgaacctc gctcagccta ctcaaaccag cgccgttcac aaccctatcg aaacaatacc
     1561 attccagacg gatatggaga cccggccaaa ccatttgaag ccaacctggg tggaggaatt
     1621 caatgtcgaa tcccatccaa tctcttcaga gatcgggacg gcaccttccc gcacgtcgaa
     1681 aaacggacag aatttccaac gattgaaaag tttaccggga atgaccgggc aacccggctg
     1741 gcggatgtcg cacttgcctg gaatatcctg caacactttt atccgtattt tgacattgcc
     1801 aatgtgaact ggatggaagt ccttcctaaa gccctcaatc tggcggcgaa tgataaaaat
     1861 gaagctgtct ttgaaaagac tttacagtgg ctgatagccc aactcaacga tggtcatggc
     1921 aatgccgttg atgccgttta tccaaccggt gcgatcccac tttcatggac gtgggctgaa
     1981 aatcaactgg tggtcacctc gctggctgta tctgatattc ccaacctgaa accaggtgat
     2041 gttgtcgtcg aagtcaacgg caaaccaacc gcgaaagtga ttgaaaagct cgaaaagtat
     2101 atttctggtg cgacaccgca atggaaacgc tttcggtctg tggacgcctt gcggtttggt
     2161 gacgccggcg acactgctac cctcaaactt cgctcggtcg agggtgatct gaaaaccgtc
     2221 aaaattgaat tctcaagtga atttctctat tacgcggagc ctcgcccaga aaagtatcaa
     2281 gaattgagcc ccggtatctg gtatttcgac ctggagcgtt gcaatgatga tgactttaaa
     2341 gcgattctcc caaaactgga atcagccaaa gggattattt ttgacggtcg cgggtatccg
     2401 tcggtaaacc cgattgtgat ccaacatctc attacccagg atgtacagtc agcgattttt
     2461 gcagtgccaa ttgtttcaac tcccgaccac gtttcaatga ctgatttcga caccaggggg
     2521 cggtggttac tcaaccccaa atcaccccac ttgaccgcaa aatgtgtctt tttgattgat
     2581 ggacgggcca ttagttacgc tgaatcgttt ttggcgatca tcgaagccta caaactcggc
     2641 gagctggtcg gagaaccaac ggctggaacc aatggaaaca ttgtgatcaa agactttcca
     2701 cgtaattttc gattggtctg gactggcatg cgagtcacaa aacacgacaa tacacctcat
     2761 cacggcgttg gggtgttgcc gacgatcccc ttatcgagaa cagtcaaggg aatttccgaa
     2821 cgccgcgatg aacagcttga gaaagcgctg tccatccttc agcaataaat gacaaagtga
     2881 cagggtgacc aggtgaccaa atgacaacat ggcaggatga aaagtgacat ttgtcacttt
     2941 gtcaccctgc cacctggtca cctggtcatc cactcactgc tggcggcgtg acagattgag
     3001 caccagtgtt tcataggtgc ggatatcatc atcgttggaa ctcaggtaat tgcctttgtc
     3061 gtcaaagagt tccacgtgcc cgtggaattt cagaaatacc ccgtaggact tgaggtacgc
     3121 gccaaaattg ctgacatcaa ttgaaaactc tccgtcgtct gaatcggcga cgaatttgta
     3181 atcagccgtg cttccggttt cagtcacata gagcgaatag cccttaaagg taatatctat
     3241 cgttcgcgca accttgccgt tggggtgaaa ttcggtgatt ttccactttc cgtcgtcaat
     3301 ttcttcaccc ggcatcgaag ccagcgtggt ggtacgaatc aattccacgg tcgaagccga
     3361 tttggcgacg ctcgcatcta cgttttgcgc cacctgcggg ccccagcctt cccaaatcag
     3421 accatctttg taaatccgta acccaaagcc agccaactga ttgccgttac aactgaagct
     3481 tgccgggtag ccatagagtg aattatcgct ctttgagaat ggcttcaggt tatagaccac
     3541 cagcgtgtct tcttctttga cttccggcca tttgttgaga tgtcggtcaa caatacacac
     3601 cgtgtatttt ccacccgctg cgattttcga gtcactgtag gtctgttgag tgcccgaggc
     3661 cggcagtttc accatctgtg aattgatctg ctgccccttg tcgtcataga tgtgaaaacc
     3721 ggcaatctgt tcaggcggaa tcaacccacg cccggtgcca actccttcaa attcccaggt
     3781 gatgctgcca gcggccacgg ttttcacggt cggcggctgc aacacatagt accgaatgtc
     3841 tatgtcagcg ctggccgtaa aattggcatt gaccagcatt tgctcaatcg aactggtggg
     3901 ttgacccgag ccgaaatgct tgatggtgat gggctttcca cctgagaagt agtttttgtc
     3961 tttgtcaatc ggggtcttgc cctgataact ggcatttgac gagccaatga aatcaaaggt
     4021 cgttgatttc aaagtattgc tggtggcggt tgatgtcccg tccataacaa acaacccgtc
     4081 gcctgaattc ttgctcgaca ggatcatatt caccatcgcc agtgagttca ctttccctgt
     4141 tagctcaaca tagtgatagg tgtcactgag aaatggcccg ttgcaggtaa tttgatcggc
     4201 aaagcctggg tcatacggat agccactcaa ccggcttttg atactttgaa ccagcccatc
     4261 catatcctcc ggatgagtca agaggtaaac cccataatcc ccaaacacct gtgtcagtcc
     4321 gtctttgatc ccacggtcag ccagttcgct cgccagatgg atcgtcatcg ggaaaccacc
     4381 catgtttttt tcacgagtga gcagtttcgc cacaaaatca ctgccataat tgttggaaag
     4441 ccattccaga aaatgcgccg ccgcatagta gctttcctcg tcattgccat cgagtgacgc
     4501 aaccaggtag tttttcatcc cgtctttggt cagaaaggtt tttttctggg catcagacat
     4561 gcccgtttgc attgccatgt agttggctga ggcttcgata aaccagattt ctgaacccaa
     4621 ccaggcggaa acatagccga gttttccagt ggtaaagtat tgaccttgaa agacgtgcgc
     4681 cagttcgtgc ccggcaacgc ctttcatata gtcacaattc tccagcttgt ttgacagcga
     4741 gagtggccca ccaattgcgc tttcccccgc cgcgtctttc agtttggaaa cataggcata
     4801 ttgtgggagc gaaagcgcgg taaacggcgt tccgggcaag gtcaggaatt tgtcataggc
     4861 ctgattgagg tagttcatca ggtccacgac aaacggctcc ggaccaccgc ctccggtgca
     4921 tccacgccag actgaggaac ctggaacgct ttcgtcatac ccaaaagccg atgggtaata
     4981 gacaatgtaa aaccgttcat tcgggtcttt tccggttac
//
LOCUS       DAOQDO010000114         5016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10848_length_5016_cov_2.704495, whole genome shotgun sequence.
ACCESSION   DAOQDO010000114 DAOQDO010000000
VERSION     DAOQDO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10848_length_5016_cov_2.704495"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKZ53_02890"
     CDS             <1..620
                     /locus_tag="PKZ53_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNJ39409.1"
                     /translation="TAAGPHPFPSMVKFFQRIIGDEARRQILEQTGKLPDYVLACVGG
                     GSNAIGIFTAFRDDAGVQLIGVEPAGKGLETPEHGAVLAKGTPGVLHGMMSLVLQDED
                     GQIFETHSISAGLDYPSVGPEHAFLKNIGAARYESATDAEAIAAFHLVCETEGIIPAL
                     ESSHAVAYAEKMARTLPEGTTLLINLSGRGDKDLQQVMNYDAQSV"
     gene            601..1404
                     /gene="trpA"
                     /locus_tag="PKZ53_02895"
     CDS             601..1404
                     /gene="trpA"
                     /locus_tag="PKZ53_02895"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNJ39410.1"
                     /translation="MTLNRYDRRFATLRETGQKAFIPFTMLGWPDRDRCLESIRLMIE
                     SGASALELGMAFSDPIADGPIIQRATFETLASGFKVKDAFALIEEIRKLDAEIPIGLL
                     VYFNTVLAKGIEGFYQQAAQAGVDGVLIADLTPEMAPQVAETALKHGIAPIFIISPLT
                     SPDRLKIITQHAGGFLYVVSRLGITGTEQRFDHALHNLLTTARQHTPIPLCVGFGIST
                     PEHARRMLEFGADGVITGSKIIQLIQEANGQPLDAVLRPFLTEMWSAVA"
     gene            complement(1411..4542)
                     /locus_tag="PKZ53_02900"
     CDS             complement(1411..4542)
                     /locus_tag="PKZ53_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ39411.1"
                     /translation="MNFAELFIRRPIMTTLVTAGILLFGFIGYRALAVSDLPNVDFPT
                     IQVTASLPGASPETMASSVATPLERQFSTIAGLSAMNSTSTQGNTQVTLQFDLSRDID
                     AAAQDVQTSISAATRQLPPGMPSPPSLKKVNPADQPILYLALTSQTLPLSKVDEFAQT
                     TIAQRISTISGVAQVAVTGSQKYAVRVQLDPTALATRGIGIDEVAQSVQQGNVNLPVG
                     TIYGTYKSLTIQANGQLVDANAYRRLIVAYRNGSPVELRDLGVVLDSIENNRVGNWLN
                     DEQVVTLAIQRQPGSNTIQVVDSIRALLPVFQQQLPAALKMEVLYDRSEAIRESFHDV
                     QFTLVLTVALVIMVIFIFLRNVRATIIPSLAVPTSIVATYAAMYLLDYSLNNLSLMAL
                     TLSVGFVVDDAIVMLENIVRRMELGEGVLEAAFNGSKEISFTILSMTISLVAVFIPVL
                     FMGGILGRLFKEFAVTISLAILVSGFVSLSLTPMLCSRFLKSGHAESHNWFLKLTERG
                     FELFLKSYEVSLRFVMQHGVATLLIAVVLTVWTAHLFIVIPKGFLISDDVGQIIGYTE
                     AAEGISYERMSENHRQLVDIIRKNPNVRAVSSTVGTSDASASSNSGKLLILLKPLHER
                     TAKADDIIIQLRPQLAQVAGIEVFLQNPPTVSIGGQVSKSLYQFTLQGSNPDTLFTSA
                     AALEQKLHTIPLIQDIASDLQVNNPQLTIKIDRDKASALGLTAQQIEDALNNSYGTRQ
                     VSTIFTATNEYQVILELKPEYQRDPTSLSLLYVRSNSGKLVPLSTVASFERDSGPLVI
                     SHLGQLPSVTISFNLVPGAALGDATEAVKKLAQQTLPSEITTTFQGTADVFQSSLTGL
                     ALLLLIAVLVIYLVLGILYESFIHPLTILSGLPSAGLGALLTLMFFKMDLSVYAFVGL
                     IMLIGIVKKNAIMMIDFALEAQRTENRPAEEAIFAACIIRFRPIMMTTMAALMGTLPI
                     ALGIGAGAQSRQPLGLAVVGGLAVSQVLTLYLTPVVYLLFEKPRLVIFGNDPEPGQPE
                     TKAVSSHHTPQEL"
     gene            complement(4686..>5016)
                     /locus_tag="PKZ53_02905"
     CDS             complement(4686..>5016)
                     /locus_tag="PKZ53_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ39412.1"
                     /translation="QNPEGVLWPGQFVNVTLTLTTQPDALVVPSRAVQTGQEGPIVYV
                     VEADQTVKIRKVTTGPTIENLTVLTSGVQVGETVVTDGHLRLIPGAKVSVKSDAEPKP
                     GPTPAPK"
BASE COUNT         1197 a         1317 c         1353 g         1149 t
ORIGIN      
        1 gaaccgcggc gggtccgcat ccatttccct cgatggtgaa attcttccag cgaatcatcg
       61 gcgacgaagc ccggcggcaa atcctcgaac aaaccggaaa actgcccgac tacgtgctgg
      121 cctgtgtcgg tggtgggtca aatgccatcg gaatttttac ggcgtttcgg gatgatgccg
      181 gggtgcaatt gatcggcgtt gaacctgcag ggaaagggct ggaaacaccg gaacacggcg
      241 ctgttttggc caagggaacg ccaggggtgc tccacggaat gatgagtctg gtgttgcaag
      301 acgaagacgg ccaaattttt gagacccata gcatttccgc cgggcttgat tacccgtcag
      361 ttgggccgga gcacgctttt ttgaaaaaca tcggtgccgc ccgctacgag tcagccaccg
      421 atgcagaagc cattgccgct ttccatctgg tgtgtgagac cgaaggcatt atcccggcgc
      481 tggaaagttc gcatgcggtg gcctatgccg aaaaaatggc ccgaaccttg ccggaaggca
      541 ctactctttt gatcaatttg tcaggtcgtg gcgacaaaga tttgcagcag gtgatgaatt
      601 atgacgctca atcggtatga tcggcggttt gcaacgctcc gtgaaacggg ccagaaagcc
      661 tttattccct ttactatgct gggttggccg gatcgcgacc gctgtctgga gtcaattcgg
      721 ttgatgattg aaagcggggc ttcggcgttg gaactgggca tggcgtttag cgacccgatt
      781 gcggatggcc caatcatcca gcgggcaacg tttgaaacgc tggcttctgg atttaaagtc
      841 aaagacgcgt ttgcgttgat cgaggaaatc cgaaaactgg acgctgaaat tccaatcgga
      901 ttgctggtct atttcaacac cgtgctggcc aaaggaattg agggttttta ccagcaggct
      961 gcccaggccg gagttgatgg agttttgatt gcggatttga cccctgaaat ggcgccgcaa
     1021 gttgccgaaa cggctttgaa acatgggatt gcgccgattt ttattatctc tccgctgacg
     1081 agtcctgacc ggttaaaaat catcactcag cacgccggag ggtttctgta tgttgtttcc
     1141 cggcttggca ttaccggtac ggagcaacgc tttgaccatg ccttgcacaa tttattgacg
     1201 accgcccgtc agcacacgcc gattccattg tgcgtcggat ttggaatttc cacccctgaa
     1261 catgcccgcc ggatgctcga atttggcgcc gatggcgtga ttaccggctc aaagattatc
     1321 cagctcatcc aggaagccaa cggccagccg ctcgatgccg tgttgcgacc atttctaacc
     1381 gaaatgtgga gtgcggtggc ttgacgccgc ttagagttcc tgtggagtgt ggtggcttga
     1441 cactgctttc gtttctggtt gtcccggttc aggatcattc ccaaaaatca ccagccgtgg
     1501 cttttcaaac agcaaataca ccactggcgt caaatacaag gtcagcacct gcgaaacagc
     1561 caggccccca accacagcca atccaagtgg ttggcgtgat tgcgcacctg ctccgatgcc
     1621 cagggcaatt ggaagcgttc ccatcaaggc cgccatcgtc gtcatcataa ttggacgaaa
     1681 ccggatgatg caggcggcaa aaatggcttc ttcggctgga cgattttcgg tgcgctgggc
     1741 ttcgagggca aagtcaatca tcataatcgc gttctttttg acgattccga tcaacataat
     1801 caacccgacg aaggcataca cgcttaaatc cattttgaaa aacatcagcg tcagcagcgc
     1861 tcccagcccg gcggaaggca gccccgacaa aatcgtcagc gggtgaataa agctttcata
     1921 caaaatcccc agcaccagat aaatcaccag taccgcaatc aaaagtaata aggccagtcc
     1981 ggtcagcgaa gactgaaaca catcggctgt gccctgaaat gtggtcgtga tttcagacgg
     2041 caaggtttgc tgggccagtt ttttgacggc ttcggtggcg tctccgaggg ctgctcccgg
     2101 caccaaattg aatgaaatcg tgacggatgg aagctgacct aagtgactga tgaccagcgg
     2161 gccggaatcg cgttcaaatg aagccaccgt gctgagcggc accaatttgc cactgttgga
     2221 acgcacatat aataaggaga gcgatgtcgg atcgcgctgg tattcgggct tgagttccag
     2281 aatcacctga tattcattcg tcgccgtgaa aattgtcgaa acctgccgcg tgccatagga
     2341 attattcagg gcgtcttcga tctgctgggc ggtcaatcca agcgctgacg ctttgtcccg
     2401 atcaattttg atggtcagtt gcgggttgtt gacttgcagg tcgctcgcaa tgtcctgaat
     2461 cagcggaatt gtgtggagtt tctgctccag tgcggcggct gaggtaaaca gggtgtccgg
     2521 gtttgaccct tgcaacgtga actggtacag gctttttgac acctgaccgc caattgaaac
     2581 cgtgggtggg ttttgcaaaa agacttcgat gccggcaact tgcgccagtt gtggccggag
     2641 ctggatgatg atgtcatcgg ctttagcggt tcgttcgtgc aggggcttga gcaaaatcaa
     2701 caacttcccc gagtttgacg aagcgctggc gtcgctggtg ccaaccgttg aagaaaccgc
     2761 cctcacattc ggatttttgc ggatgatatc caccagttgc cggtggtttt cggacattcg
     2821 ttcgtaggaa atgccttcag cggcttcggt gtaaccaata atctggccga cgtcatcgct
     2881 gatcaaaaat ccttttggaa tcacaataaa caaatgcgcg gtccagaccg tcaggaccac
     2941 cgcaatcaac aaggttgcga cgccgtgttg catcacaaac cgcaggctca cttcataact
     3001 tttcaaaaac aattcaaacc cgcgttcggt caacttcaag aaccagttgt gcgattcggc
     3061 gtgtccactt ttcaaaaacc ggctgcacaa cattggcgtc aaactgagcg aaacaaaccc
     3121 tgaaaccaga atcgccagac tgatggtaac ggcaaattcc ttgaacaacc gtcccaaaat
     3181 gccacccata aacagcaccg gaataaacac tgcgaccagc gaaatggtca tggacagaat
     3241 cgtgaacgag atttcctttg agccgttaaa tgccgcttcc agaacacctt cgcccagttc
     3301 catccgacgc acgatgtttt caagcatgac aatcgcgtca tcaaccacaa atccgaccga
     3361 gagcgtcagt gccatcagcg aaagattgtt caaactgtag tcgagcaaat acatcgccgc
     3421 ataggtcgcg acaatcgagg tcggcaccgc cagactggga atgatcgtgg cccgcacatt
     3481 tcgcaggaaa atgaagatca ccataatcac cagcgccacc gtcagcacca gcgtgaattg
     3541 cacatcatga aatgattcgc ggatggcttc cgagcggtca tagagcacct ccattttgag
     3601 cgccgccgga agttgttgtt gaaagaccgg taacagtgcc cggatggaat caacgacctg
     3661 aatagtgttt gaaccgggct ggcgttggat ggccagtgtc accacctgct catcattgag
     3721 ccagttgccg acgcgattgt tttcgatgct gtcgaggaca acgcccagat cccggagttc
     3781 aaccggcgag ccattccgat aggccacaat cagtcgtcgg taggcattgg catccaccag
     3841 ttgaccgttg gcctggatcg tgagcgactt ataggttccg taaattgtcc cgaccggcaa
     3901 attgacgttt ccttgctgaa cagattgcgc aacttcgtca atcccaatgc cccgcgtcgc
     3961 cagtgctgtc gggtcaagct gcacccggac ggcatacttt tgcgaaccag tcaccgccac
     4021 ctgcgcgacg ccgctgatgg ttgaaatccg ctgggcaatc gtcgtctggg caaactcgtc
     4081 aacttttgaa agtgggaggg tttgtgaggt cagcgccagg tacaaaatcg gctgatccgc
     4141 cgggttgact ttctttaatg atggcgggct gggcattcca ggtggaagtt gtcgcgttgc
     4201 ggctgaaatt gaggtctgaa catcctgtgc cgccgcgtca atgtcacggc ttaaatcaaa
     4261 ctggagcgtg acttgagtat tgccctgagt actggtcgag ttcatcgccg aaaggccggc
     4321 aatggtcgaa aactgacgtt cgaggggcgt cgccaccgaa gacgccatgg tttccggact
     4381 ggcacctggc aggctggcgg tgacctggat ggttgggaaa tctacgtttg gcaggtcact
     4441 gaccgccagc gcccgatagc cgataaagcc aaacaacaaa attcccgccg tcaccagcgt
     4501 ggtcataatc ggacggcgga taaacaattc agcaaaattc atggcagatc tctttttcac
     4561 cacagaggac acagaggaac acagaggaag acagaatgaa gaattgagaa tgaagaatga
     4621 agaaatcttt taattcctgg tattcctctg tggtccgctg tggtgagttt caactttccc
     4681 acacatcatt ttggagcagg cgtgggccct ggttttggtt cggcatcgga tttgacactg
     4741 accttggcac ctggaatcag ccgcaaatgt ccatcagtga caacggtttc accaacctgc
     4801 acgccgctgg tcaatacggt gagattttca atggttggcc cggtggtcac ttttcggatt
     4861 ttgacggttt gatcagcctc gaccacatac acgattgggc cttcctgacc ggtttgaacc
     4921 gcccgcgaag gcaccaccag cgcatctggc tgggttgtca gcgtcagcgt gacattcaca
     4981 aactgacctg gccacaacac accttccggg ttttga
//
LOCUS       DAOQDO010000115         5013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10856_length_5013_cov_3.259379, whole genome shotgun sequence.
ACCESSION   DAOQDO010000115 DAOQDO010000000
VERSION     DAOQDO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10856_length_5013_cov_3.259379"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PKZ53_02910"
     CDS             complement(<1..1434)
                     /locus_tag="PKZ53_02910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNJ39413.1"
                     /translation="MLKNKLVIWFATGWLCVGLVGNHVTAQEPHALPERENQVPQAWV
                     NHQLRPLRKFGQPQLPALFPSSFDRWRISPQPQYLSSAALPVRVSQNLVPSDRGSQAE
                     TQAEPHVAVNPDNPTNLIATYQDSRFANGGALALTYATSFDGGQTWIENVVPGLSRVS
                     GGSWDRATDPWVAFGSDNRAYLVSLAFQERSPENEIAVSSSTDGGLTWGSPVTVIRSI
                     DNFNDKETITVDTSPASPFRGNVYVTWDANVFGSDGVTIRQELVLSRSTDQGQTWSDP
                     IKIRRKGANIGVIPRVGPDGSLYTVWAGGSIQGNRIWLFFSKSMDAGQTWSKPKKLQE
                     MTNRGIAGARTGDFLPSFDVDPVSGELFVGWQDARWTGIDQVTLISSRDKGETWTAPR
                     RVSSVTDDAPCFTVSVATNPNGDVAVSYYSFAHDPERRFLTDLFLQVSTDGGKTFGTP
                     QRVTPASFDLRYAAQARGFFLGDYIGLA"
     gene            complement(1621..3366)
                     /locus_tag="PKZ53_02915"
     CDS             complement(1621..3366)
                     /locus_tag="PKZ53_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ39414.1"
                     /translation="MRKKFSVAVLLALMMWFPVVAINVTTPEGVLDNPAGGGDTLNDP
                     FVADTWLRLNVRDGSTVGITDNYPRGGTASAYLSGTVANTSKADFEYYWSDTTGKTLG
                     NLTNLQYEWYRDPASTNPDVQHPAFRLAIDVDGNLATTTDRGYLIYEGAYNGLAVAPE
                     GVWQTVNITTSNFWFRWFGPPGQTCEVYDQTLAEWQSNPPPGACSGFPDISSSTLVTG
                     ISFGIGSGWNGVFQGAVDNVELTFAGQDPLAFNFEFPPTPVLTNTLYVADTTNNRVQV
                     FDSATATWNLVVGTDGVTGAAPNRFNRPEGVVANNGTGVDLRVYVADTGNNRIQWFDG
                     TTWGTFASVGSSLAQVRAPEALTLDSEGNLYVADTGNDRILRFATNPDGSPAGNATAL
                     ATVGFLRNKVSGPRGLAIDSAFNLFIADTDNDRILKLLTANDPGYTNATPPPLANFEI
                     IATSGGSINPGKVRDPEGVAVDAAGNLYVADTGNNRVIEFIGATPGPVTVLASSGSTL
                     GGAIGKVRLPEGITVTDFSGVETLLVADSGNNRVQQFPLAGGTWEAVSGTPNPPQTGQ
                     SGSSIGQFRAPSGVR"
     gene            complement(3883..>5013)
                     /locus_tag="PKZ53_02920"
     CDS             complement(3883..>5013)
                     /locus_tag="PKZ53_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNJ39415.1"
                     /translation="AKVAFKYNGAHRELTPEQQAAFEAEGRPFVIRFKVPQDGGAVAF
                     EDLVYGVQEKHHADIEDFVIMRSDGTPLYLLSNMVDDADQGVTHVIRGQDGLSNTPKQ
                     VLLYQALGKPVPKFAHLPLILDPKRAKIGKRKHGNVVTVRYYQERGFIPDAFLNFLAL
                     LGWSTGDDREILSLDDMREMFTFERVSHTNAVFNLNTTDPRIGTDPKALWMNAEYIKT
                     WPLEKLLPLVREQLEKAGLWQESYAGDQAEWFAKTVDLLRARFRVLTDFVTLGRPYFA
                     DDFEFDPDAVKKNLKDASLKELLPGLADAFETLTEFSHDTVEAALRAFAEEKGVKAGL
                     LINGSRTAVSGQSVGPSLFELLVTVGQERTCRRLRAVVDQIA"
BASE COUNT         1230 a         1371 c         1233 g         1179 t
ORIGIN      
        1 ggccagacca atgtaatctc ccagaaagaa ccctcgtgcc tgggcggcat agcgcaaatc
       61 aaacgaagct ggggttactc gttgcggcgt cccaaacgtc tttccgccat ccgttgaaac
      121 ctgaagaaac aggtcagtca gaaagcgtcg ttccgggtca tgagcaaagc tgtagtagct
      181 caccgcaacg tcaccgtttg ggttggtggc cactgaaacc gtaaaacatg gggcatcgtc
      241 agtgactgaa ctgacgcgtc ggggcgctgt ccaggtctcg cctttgtcac gcgaagaaat
      301 gagtgtaacc tgatcaatgc ctgtccagcg ggcatcctgc cagccaacga acaattcccc
      361 tgaaactgga tccacatcaa atgagggcaa aaaatcaccc gtccgggcac cagcaatgcc
      421 tcgattggtc atttcctgca attttttggg tttcgaccag gtttggccag catccatact
      481 ttttgagaaa aaaagccaga tccggtttcc ctggattgag ccacccgccc acaccgtgta
      541 caggcttcca tccgggccca ctctgggaat caccccaata ttggcgcctt tgcggcggat
      601 cttgattgga tcgctccagg tttgcccctg gtcggtcgaa cgagaaagaa ccagttcctg
      661 acgaatggtc accccatccg aaccaaacac attggcatcc caggtgacat agacatttcc
      721 tcgaaaagga ctggccgggc tggtgtcaac ggtgatggtt tctttgtcgt taaaattatc
      781 aatggagcgg atgacggtga cggggcttcc ccaggttaat ccgccatccg ttgaggaact
      841 gacggcaatt tcattctcgg gtgatcgctc ttgaaaggcc agactcacca gatacgctcg
      901 attatcagat ccaaaagcca cccacggatc cgttgcccga tcccaggaac ctccgctaac
      961 ccgactgagc cctggcacca cattttctat ccatgtctgg ccgccatcaa aactggtcgc
     1021 ataggtcaac gccagggcac ccccattggc aaaccggctg tcctgatagg tggcaatcag
     1081 attggttggg ttgtcaggat tgacggccac gtgcggctcc gcctgggttt cagcttgcga
     1141 accacgatcc gaaggcacca gattctgact gactcgaact ggtaatgcgg cactactcag
     1201 atattgaggc tggggtgaaa ttcgccaccg atcaaatgaa gatggaaaga gagctggcag
     1261 ttgaggctgc ccaaatttgc gaagcggtcg cagttgatga ttcacccagg cttgtggaac
     1321 ctgattttca cgctccggta aagcgtgtgg ttcctgagcc gtcacgtgat ttcctacgag
     1381 cccgacacac aaccacccgg tggcgaacca gatcaccagt ttgtttttca gcatagattt
     1441 ttgggtttcc aaagagaatt ggcctggaat tgttcgatga ggagtgactc aaactctggc
     1501 tggttgtcac gcaatttgca atcgctttcc tctgcaggct ctcaaaaaat atcgcgtggc
     1561 cacctttgcc aggtacccac gcgatgaatg tgcttttgtg aagcaaggtt ggattttttc
     1621 ttagcgcaca ccactcggcg cacggaattg tccaattgag ctaccagatt gtccggtttg
     1681 aggcgggttc ggagtgccgc ttacggcttc ccaggtgccc ccagccagtg gaaactgctg
     1741 gacacgattg tttcccgaat ctgccaccag caatgtttca accccactaa aatcagtgac
     1801 cgtgattccc tccgggagcc gaactttccc aatggcacca cccaatgtcg aaccggaact
     1861 tgccagaacc gtcacaggcc ctggtgtcgc accgataaac tcaatcaccc ggttgttacc
     1921 ggtatcagcc acgtacagat ttccagccgc gtcaacggca acaccttctg gatccctgac
     1981 ctttccagga ttgattgacc caccgctcgt ggcaataatt tcaaaatttg ccagcggcgg
     2041 cggggtggca ttggtatatc caggatcgtt ggccgtcaac aatttcaaaa tccgatcatt
     2101 gtcggtatca gcaatgaaca gattgaaggc tgaatctatg gccagtcctc gggggccact
     2161 gaccttattt cgcagaaatc cgacagttgc cagcgcggtc gcgttcccgg cggggcttcc
     2221 atccggattg gtcgcaaacc gtaaaatccg atcattgccc gtatctgcca catacagatt
     2281 gccttcacta tcaagggtca gcgcttccgg ggcccgaacc tgggccaggc ttgacccaac
     2341 cgaagcaaat gtcccccagg ttgttccatc aaaccactga atccggttgt tgccggtatc
     2401 agccacatac acccgaagat caaccccagt cccattattg gcaactacgc cttcagggcg
     2461 attaaaccga ttgggcgccg ctccagtcac cccatccgtt ccaacaacca gattccaggt
     2521 tgcggttgca gaatcaaaga cctgaactcg attgtttgtg gtatcagcca catagagcgt
     2581 attggtcaac actggagttg gcggaaattc aaaattaaaa gccagcgggt cctggccagc
     2641 aaaggtgagt tcaacattgt caacggcgcc ttgaaacacg ccgttccatc cagaaccaat
     2701 cccaaagctg atcccggtta ccagagtgct gctgctgata tcggggaatc ctgagcaggc
     2761 gcccggagga ggattcgact gccattcggc cagcgtttga tcataaactt cgcaggtttg
     2821 cccaggcggg ccaaaccagc ggaaccaaaa attagaggtt gtaatattaa cggtttgcca
     2881 gacaccttcc ggtgccacag ccaacccatt ataggcgcct tcataaatca aatacccacg
     2941 gtcagttgtg gtggcgagat taccatccac gtcaattgcc agccgaaacg ccgggtgctg
     3001 aacatccggg ttggtactgg ccgggtcgcg gtaccattca tactggagat tcgtcaggtt
     3061 tccaagggtt tttccagttg tatctgacca gtaatattca aaatcagctt tggatgtatt
     3121 ggcaacagtg cctgataaat aggctgaggc ggttccgccc cgtggataat tgtcagtgat
     3181 tccaacggtg gagccatcgc ggacattgag tcgtaaccag gtatcagcca caaagggatc
     3241 atttaaggta tcacctccgc cggctggatt gtctaatacg ccttcaggtg tcgtgacgtt
     3301 aatggcaacc acagggaacc acatcatcaa agcaagcagg acagcaacgc taaatttctt
     3361 tcgcatatgg gtttctcctg ggatgtgcga ctttcaccaa atccttccca cattggaacc
     3421 tggcggcatc aacctatcag ttcatccaaa accaataact ggcaaaccaa ctggcggcat
     3481 caccgttttt ccagatgacg acagatagat ttctgggatt gagattttta acctggttca
     3541 aaaagctttg gattgtgctc aagaaacgtc aatcttcggc tggtttgaat tcaacaacgg
     3601 gttcgactgg ggggaagaat acccgttgag aacataagga tctggtgaat attgagtcaa
     3661 ttgggtttga ctctttatgt taagggtaga gcgactctat catccagcaa ttggattcac
     3721 caaaatatat tgcctgattt gacttcaaat tcttggaatt tccagcaaat tggcgggtta
     3781 ttccagaaac catcctcaaa tgtcaaaagc cgattcactc acttgaatga atcggctttt
     3841 ttcgacactt cttttcagta cagaacatca agaaacaatc attcaggcaa tctgatcaac
     3901 cacagctctt aatcggcggc aggttcgttc ctgaccgacg gtaaccagca gttcaaacag
     3961 gctcggtcca actgactgtc ctgaaactgc cgttcgtgaa ccgttgatga gcaatccagc
     4021 tttgactccc ttttcttcag caaaggcccg gagggcagct tcaaccgtat cgtgtgaaaa
     4081 ttcggtcaag gtctcaaacg catcagccag cccaggcagc aattctttga gcgaggcatc
     4141 tttgaggttt ttcttgaccg catctgggtc aaattcaaaa tcatccgcaa aataaggccg
     4201 ccccaaagtc acaaaatcgg tcagcacccg gaaacgcgcc cgcagcaagt caaccgtttt
     4261 ggcaaaccat tccgcctggt ctccggcata gctctcttgc cacaacccgg ctttttcaag
     4321 ctgttcacgc accagcggaa gcagtttttc caacggccag gttttgatgt actcggcatt
     4381 catccacagg gctttcggat cggtgccaat ccgtggatcg gtggtattga ggttaaacac
     4441 ggcattggta tggctgacgc gttcaaaggt aaacatctcg cgcatgtcat cgagcgacag
     4501 aatttcgcgg tcatcaccgg ttgaccagcc caggagcgcc agaaaattca aaaaggcatc
     4561 cggaatgaac ccgcgttcct ggtaatagcg cacggttacc acattgccgt gtttgcgctt
     4621 cccgattttg gctcgctttg gatccaaaat cagcggcagg tgggcaaatt tcggcactgg
     4681 ttttccaagt gcctgataga gcaacacctg tttcggagta tttgacaacc catcctgacc
     4741 gcgaatgaca tgcgtcacgc cctggtcagc atcatccacc atattgctga gcaggtagag
     4801 cggtgttcca tccgaccgca taatcacaaa gtcttcaatg tcagcatgat gtttctcctg
     4861 cacaccgtag acaaggtctt caaatgccac ggcgccgcca tcctgcggga ctttgaaccg
     4921 aatcacaaac gggcggcctt cggcttcaaa ggcggcctgt tgctcggggg tcagttcgcg
     4981 gtgggcaccg ttgtatttaa acgcgacttt ggc
//
LOCUS       DAOQDO010000116         5003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10897_length_5003_cov_3.114592, whole genome shotgun sequence.
ACCESSION   DAOQDO010000116 DAOQDO010000000
VERSION     DAOQDO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10897_length_5003_cov_3.114592"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            526..4167
                     /locus_tag="PKZ53_02925"
     CDS             526..4167
                     /locus_tag="PKZ53_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ39416.1"
                     /translation="MYQKLQGNTITKVLTGLVFTLGLVFLLSNAPATLAQGTNGRLVV
                     TVKDQTEAVVAGATIKVVNQGTSQEITGTTNDSGVSIFPQMAVGLYTVKVEVPGFKTA
                     AREDLKIDVGQEYGIVISLEVGSEGDVVTVTAGEELVQTTTSEVRNTVSEKQVQELPI
                     NGRDPLQLIQLQAGVNSGVNNARAQATTTINGQRASTANLTQDGINIQDNLLRRNSLD
                     FSPNRPSVAQIAEFTVTTQNGGAEVSGGASAIRAVSPNGTNEYHGSLFEYHRNSALGA
                     NDFFNNLQEVAKPQLIRNQFGGTVSGPILKEKLFFFGFYEGLRERIGTPFTGTVLLPN
                     ARQGVFTYRDNSGQTRTINVLGLRNLNIDPAIANILNASPQSANATTVGDGLNTSGFA
                     LNRSFNQDRNVGGFRLDYVLNSNHRFEGVFNRATELVDRPDADVSFAAKPGANNNLLT
                     YFAVGAWNWQITPNLTNELRIGGNRPEGNFGSNADLNSVFLTLPLITNPNNQFTPEGR
                     TTSTISYIDSMSYTRGDHFFRFGGQFNRIRTNPFVFNAGTPPTFTVGFGVGAPDEIPL
                     TSRDFPGGIDPTQLNTANNLLALLSGALADGQVNYEVTSQNSGYVSGIPNRRNYEYKE
                     YSGYFTDSWRTHPRLTVNLGLRWDYRTPLKERDNLLLAPVLNGRNVREAVLDPNGSYG
                     FVDGFIANPDRANFAPNISFAWDIPGLGRQTVLRGGYSISYVNDQAIAAPRNALEGND
                     GLSAVVNRNDIFGYLSRDVQNLLQTSFGAPQFVVPRTYLQNFDLDNTAAAFAVNPDIK
                     TPYFHQWNVSIDREISNNMVLSLRYVGNKSTNLIRGVDFNQVDVINNGFAADVLRAQS
                     NGFLALAQNGRFDPRFNPNIAGSQQLLVFPKLAGGGLLTNTGTIQPLIQRGEAGTLAQ
                     VYHQFGLAGGVSFVPNSNIFVADVLDNGAESNYHALQVEMRRRFSNGLTFQANYTWSK
                     NLTTSPGTNAQILFEPLLDNNQPGLEYARSILDIPHTLKANVIYELPFGPGKRFNPNN
                     AILRKVVGGWQVTSIITVQSGSPFSIVSGRGTLNRGGRSLTTNTANTSLTNEQIKDLI
                     GFFPTENGIYLINPSILGTDGRAVSPDGRGPFAGQVFFNPGAGQVGSMARQAFNNPRF
                     SNWDFSVIKRTPITEKIDTEFRAEFFNFTNSPMFGIEDPNINSTVFGQARFQLNSPRI
                     VQLAFKIVF"
     gene            4371..>5003
                     /locus_tag="PKZ53_02930"
     CDS             4371..>5003
                     /locus_tag="PKZ53_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ39417.1"
                     /translation="MAKLHFLGAAGTVTGSKFVLEANSSRVMIDCGLFQGLKELRLHN
                     WDPLPINPASINWVVLTHGHIDHTGYLPRLVRDGFDGPVYATQGTEELVDLLLPDSAR
                     LQEEDAEYANKKHYSKHEPALPLYTEQDARVALRKVVGFPYNQEFQLGKHMSGRFISA
                     GHILGSSFVSIEVSERHASPFTILFSGDLGRYDEPILNDPTAVPEADYLLV"
BASE COUNT         1227 a         1348 c         1216 g         1212 t
ORIGIN      
        1 tggcgtttgc caatgtggcg agcccctcgt ttaacaaccc gcgtgtgttt gcgggtcgtc
       61 cacgaaccat tcagttttgt ctcaaactga tcttttaatg gatggcggga aagcaagcgg
      121 gtctgaccgg tttatgttgg atgggtgtga aaaccgacgg acttcttgct ttccggcctt
      181 catcgcctgg aatgggtctc ctgaaagccc gaagggacgg caggttctga cgaatgctga
      241 tcaaagctga atctggaacc tgccatcttt tacttatcac acaacaaaac taaaacatta
      301 aaacgttttt cattaaaaac ttatttatat tcttaatagc cagtagtacg tgtaccgact
      361 caccacaatt gaaagctctc atacaaactg aaaatacgtt gagagggtga ggctgaccaa
      421 gaacgcctca cacgccaaaa caaaaaagtg ttcatccggt gaagtttgga agacgccacg
      481 ggatgaacac aacggtaaca gtagacgaat tacctggagg tttttatgta tcaaaaattg
      541 cagggaaata ccatcacaaa ggtgctgacc ggactggttt tcacccttgg tctggtgttt
      601 ctactttcaa atgctccagc cacattggcc cagggcacca atggtcgcct ggtggtcacc
      661 gtcaaagacc agacagaggc ggtcgttgcc ggagcaacta tcaaagtcgt caatcaggga
      721 acgtcccagg aaataacggg aacgaccaac gactctggag tgagtatctt tccacaaatg
      781 gcggtgggcc tttataccgt caaagtcgaa gtcccaggct ttaaaactgc cgcgcgtgaa
      841 gatctgaaaa ttgacgtcgg gcaggaatat gggattgtta tttcgcttga agtcggttcg
      901 gaaggagatg tggtgacggt caccgccggt gaagaactgg ttcagaccac cacgtccgaa
      961 gtccgcaaca ccgtgagtga aaagcaagtc caggaattac caatcaatgg tcgtgacccg
     1021 ttgcagttaa tccaattgca ggctggtgtc aactcaggtg ttaacaatgc ccgagcacag
     1081 gccaccacca ccatcaatgg tcagcgtgca tcaacggcca acctgaccca ggatggcatc
     1141 aacatccagg acaacctgct gcggcgcaat tcgctggatt tctcgcccaa ccgtccatcc
     1201 gtggcgcaaa tcgctgaatt taccgtgaca acgcaaaatg gcggcgctga agtttcgggt
     1261 ggtgcttcgg ccattcgtgc ggtttcccca aacggcacca acgaatatca tggctcgctc
     1321 tttgagtatc atcgcaacag cgcgctcggc gccaatgact ttttcaacaa cctgcaagaa
     1381 gttgccaaac cccagttgat tcgcaaccag tttgggggca cggtttcagg cccgatttta
     1441 aaagaaaaac tgtttttctt tggtttttat gaaggcttgc gggaacgcat cggcacacca
     1501 tttaccggaa ccgtgttgct tcccaacgcc cggcaaggtg tgttcaccta tcgtgataac
     1561 tccggtcaaa cccggacgat caacgtcctt ggtttgcgaa atctgaatat tgacccggcg
     1621 attgccaata tcctgaatgc ttccccacaa agcgccaatg ccacaaccgt tggtgacggg
     1681 ttaaatactt caggctttgc gctcaaccgg tcatttaacc aggaccgcaa tgtaggtggg
     1741 ttccgcctgg attatgtcct gaacagcaat catcggtttg aaggcgtctt taaccgggca
     1801 accgaactgg ttgaccgacc agacgctgac gtttcttttg ctgccaaacc aggtgccaac
     1861 aacaacctgc tcacctactt tgccgtgggt gcgtggaact ggcaaatcac acccaatctg
     1921 accaacgaat tgcgcatcgg cggcaaccgc cccgaaggga attttggttc aaacgccgat
     1981 cttaacagcg tctttttgac gttgcctctc atcaccaacc caaataacca gtttacacct
     2041 gaaggccgga ccacctcgac gatatcttat atcgacagca tgtcctatac ccgtggtgac
     2101 cacttcttcc ggtttggtgg tcagtttaat cgcatccgca ccaacccatt tgtgttcaat
     2161 gctgggacac caccaacgtt tacagtgggc tttggtgtcg gtgcaccaga tgagattcca
     2221 ctgacttcgc gtgacttccc gggtggcatt gacccaactc agttgaatac agccaacaac
     2281 ctgctcgcgc tcttgagcgg cgcgctggct gatggtcagg tcaactatga agtgacgtcc
     2341 caaaactcag gttatgtgtc gggtatcccc aaccgacgta actatgaata caaagagtat
     2401 tccgggtact ttactgattc ctggcgcaca catccgcgcc tcacggtcaa tctgggactc
     2461 cgctgggatt accggacacc actcaaggaa cgtgacaacc tgttgctggc accagtcctg
     2521 aatggccgca atgtccgtga agccgttctg gatccaaacg gttcctatgg atttgtagat
     2581 gggtttattg ccaatccaga tcgagccaat tttgcgccaa atatcagctt tgcctgggat
     2641 attccagggt tgggccgtca gacggttttg cgcggtggtt attcgatttc ctatgtgaat
     2701 gaccaggcga ttgccgcacc acgcaatgcg ctggaaggca acgatggact gagcgccgtg
     2761 gtcaaccgca acgacatttt cggctacctg agccgcgatg ttcaaaacct gctccaaact
     2821 tcatttgggg cgccacaatt tgtggtacca cggacgtacc tgcaaaattt tgatctggac
     2881 aacaccgcag cggcgtttgc ggtcaacccg gatatcaaaa caccatattt ccaccagtgg
     2941 aacgtcagta ttgaccgtga aatttccaac aacatggtct tgtcgctccg ctatgtcggc
     3001 aacaagagca cgaatctgat tcgtggtgtt gactttaatc aggtggatgt catcaacaat
     3061 ggttttgcgg cagacgtgct gcgtgcacag agcaatggct tcctggccct ggctcagaat
     3121 ggccgctttg atcctcgctt taaccccaac attgctggca gccagcaact gttggtgttc
     3181 ccgaaactgg ctggcggtgg attgctgacc aacacgggta cgatccaacc actgattcag
     3241 cgcggagaag ccggaacact ggctcaggtc taccatcaat ttgggttggc cggaggcgtc
     3301 tcgtttgttc caaactcaaa cattttcgtg gctgacgtgc tcgacaacgg tgctgaatcc
     3361 aactatcacg cactgcaggt tgaaatgcgg cggcggttct caaacgggtt gacgttccag
     3421 gccaactaca cctggagcaa aaacctgacc accagcccag gtaccaatgc tcaaattctg
     3481 tttgagccat tgctggacaa caaccagcca gggttggaat atgcccggtc catcctggac
     3541 attccacaca ccctgaaagc caatgtgatc tatgaattgc catttggccc aggaaaacgg
     3601 ttcaatccaa acaatgcgat tctgcgcaaa gttgtgggtg gatggcaggt aacctcaatt
     3661 atcaccgttc agtccggcag tccattctca atcgtgtcag gacgcggcac actcaatcgt
     3721 ggcggtcgct cactgaccac caacaccgcc aacacgtcat tgaccaacga gcaaatcaaa
     3781 gatctgatcg ggtttttccc aactgaaaat ggaatttacc tgattaaccc atccatcctc
     3841 ggaacagatg gtcgcgcggt ttccccagat ggtcgtggcc catttgcggg acaggtcttc
     3901 ttcaacccag gcgctggcca ggttggttca atggctcgtc aggcatttaa caacccacgc
     3961 ttctcaaatt gggacttcag cgtgatcaaa cgcaccccaa tcactgaaaa aatcgatact
     4021 gaattccgcg ccgaattctt taacttcacc aactcgccga tgttcggaat cgaagatcca
     4081 aacatcaaca gcacggtgtt tggtcaggcg cggttccagt tgaacagccc acgcatcgtt
     4141 caattggcgt tcaagatcgt gttctaacct ctttcttgtt gttgtgacgg tctcttttac
     4201 ggcctgcccc ctctgttcca tcgggggggc aggccatttt ttcgtttcaa gggagggagg
     4261 tttcaggtat tcttctttca ataatcgccc ataccagatc ccccaaccat aatcttctgg
     4321 cttcaacact cacccatcgc agcccaaaaa cctgaaaatt ttaaaaattt atggcgaaac
     4381 ttcattttct tggtgctgcc ggcaccgtca ccggctcaaa atttgtcctg gaagccaaca
     4441 gcagtcgcgt catgattgat tgcggcttgt ttcaaggctt aaaagagctt cggttgcaca
     4501 actgggatcc attgccgatc aatccggctt caatcaactg ggtagtcctc acccacgggc
     4561 atattgacca taccgggtac cttccccgcc tggttcggga tgggtttgac ggcccggtgt
     4621 atgccaccca gggaactgag gaactggtgg atttattgct tccagactcg gcccggctcc
     4681 aggaagaaga cgccgaatat gccaacaaaa agcactattc caaacacgaa cctgcccttc
     4741 ccctctatac cgagcaggat gcccgggttg cgctcaggaa agtggttggc tttccttata
     4801 atcaggagtt tcagcttggc aaacatatgt caggccggtt tatttcagcc ggacatattc
     4861 ttggatcgag ctttgtttcg attgaggtga gcgaacgcca tgccagcccc tttaccatct
     4921 tgttttcggg tgacctgggg cgatatgacg aaccaattct gaatgacccg acggctgttc
     4981 ccgaagctga ttatttgctg gtt
//
LOCUS       DAOQDO010000117         5000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10909_length_5000_cov_2.678665, whole genome shotgun sequence.
ACCESSION   DAOQDO010000117 DAOQDO010000000
VERSION     DAOQDO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10909_length_5000_cov_2.678665"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKZ53_02935"
     CDS             complement(<1..645)
                     /locus_tag="PKZ53_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNJ39418.1"
                     /translation="MINHTTADQCRNLPELVYFHTCRQPDKTFLISETDGRTFTYAEF
                     TASIHRTARLLASHGIQKGDVVSLLMPNSVEYVIAYFACFQLGALAGPVNSLLKAHEL
                     TYVIGNSEARAILVNSQFESVIDDIKHDLPALKSIIRFDLESDATADLQVADCPEPEI
                     DREDEAIIIYTSGTTGKPKGCLLTHGNLLANARQIKNWLGFTESDRLLTIMPLFH"
     gene            complement(982..2526)
                     /locus_tag="PKZ53_02940"
     CDS             complement(982..2526)
                     /locus_tag="PKZ53_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNJ39419.1"
                     /translation="MTHLTYICGLLLVLFSPFSLFAKPTHPIAPQSKSKTVSILFFND
                     AHQVMPVSDQLGIRGGVARLKTIIDQVKRDNPSTLVAFGGDLAGGLLFGAVFHGHPQV
                     AAFNQLPVDVASFGQHDFDFGSAETRKLIAESKFNWITSNLVEQSGQPFAGVPRYLIR
                     ESGGLKIGFIGLTDDFDTTLPENIVTQQNILQSAKSATSALRKQGADVIVALCQADMP
                     KNLQMLEQVPDIDAIFGEEQFENRSIIQFVNNRPALSPCGNAGSVVQLDVTLNQGKCV
                     FSVRVHSIDETVASAPDLLKFEQRYQTELDTKLAEPLAQVKTPLLAGYNTDNALRRRE
                     TNLGNFVADAFRHHFGTEIGIINSGGIRAEAEGPVFTLKNAVSLLPFGNRLVVAKYTG
                     AQLRQALEHGTSRVEMSGGRLLLVSGLSYEFDLRAPVGKRIQSVKVQGKPLKDDQVYR
                     VALPNFLQLGGESFDMLKTDTIEPEPPAGWPLDVDVLSEYARTQKVIDIGIQGRIVVR
                     DSTNSE"
     gene            complement(2957..4861)
                     /locus_tag="PKZ53_02945"
     CDS             complement(2957..4861)
                     /locus_tag="PKZ53_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNJ39420.1"
                     /translation="MILKAIGSFLLLCLLVVPASALQKKKPPSFRHQISTQSEFDTLK
                     RIYYRGRFASIPHLMFVIDRNEKTIAYVNSNLYTFHTDFVFSNYWSLERGAKFFEKNY
                     LNPNRRFILGTLAYQTEAKLFTFEFWEGDLIPKELVEYTFSTIQKSFFAPIKFKPNSL
                     RQEQLSDELPTVPRILISELEPFNTEEIIHPGETVGILRLRENLPDEQPVEPEDIVIF
                     KQVPISVTPISGIVTTRPGTPLSHLNLLSKTWNIPNVFVKDADEKYKALLNKWVRLKA
                     RNDEIVIEPVDVNAAKRLSPQVAKRLQLQVPRADLSWNQLTDLAQQRSRDVVRFGAKS
                     ANLGQVAQAKIKGVIVPPGFTIPFACYVEFAKKIGLEERVLELLNDEHFHHDPKIRKV
                     KLAELRAYVQTSPMPEALKIAILEKVHREFPTAGLFVRSSTNAEDLPNFNGAGLYTTV
                     PNVKGDDALIEAVKTVWASIWNFEAFEAREAAKIDHLAVYPAVLLQTGINAESAGVMI
                     TTNPFDPQDTGGVYINAKRGLGIKVVEGQKIPEQIIFSTRSNAVRVLSRSGEDSLLTF
                     DQNGGVKEIQLFSDRAILTDANARRLSAAALKIKHLFNQKEQDIEWALVGDQVYILQS
                     RPYIDPLARR"
BASE COUNT         1247 a         1170 c         1290 g         1293 t
ORIGIN      
        1 atgaaacagc ggcatgatgg tcagcaaccg atccgattcg gtaaagccaa gccagttttt
       61 gatctggcgg gcgttggcca gcaggttgcc gtgggttaac aaacagcctt tgggtttgcc
      121 ggtggtgccc gaagtataaa taatgatggc ttcgtcttcg cgatcaattt cgggttccgg
      181 gcaatcggcc acttgaagat cagccgtggc atctgattcc aaatcaaacc gaataatgga
      241 tttcagggct ggcaggtcgt gtttgatgtc atcaatcacc gactcaaatt gactgttgac
      301 caggatggcc ctggcttcgg aattaccgat gacataggtc agttcgtggg ctttgagcaa
      361 tgaattgact ggcccggcca gtgccccaag ctgaaaacag gcaaagtagg caatcacata
      421 ttcgacactg ttgggcatca gcaaactgac cacatcgcct ttttgaattc cgtggctggc
      481 taacagccgg gctgtccggt ggatggacgc ggtaaactcg gcataggtaa aggtacggcc
      541 atcagtttct gaaataagaa aggttttgtc gggttgtcgg caggtatgaa aataaaccag
      601 ttccggaagg tttcgacact ggtcagcagt tgtatgattg atcatttgtt gtcggtcctc
      661 acgccaggtg tggtatagtt gccgctcgcc aaaattgaat tatgaattat gaattatgaa
      721 ttatgaaaca gctaactatt tgctactcat accaatttgc aatgaaatcc tcgtattagc
      781 aaacagtcaa gtgattcaat caaataaatt tagtgaacta ctgactacgg gctactgact
      841 acaaactggc attatcttca gtcccaaaat ccttccacca ccactttaac tatccagcac
      901 ctcaaaaaaa gaaaatgagg agcgagtttt gggttgacaa ctcgctcctc gctccttgct
      961 tcttgctctt ttgcgctttc actattcgct atttgtgctg tccctgacca cgatgcgacc
     1021 ttgaatgcca atgtcaatca ccttttgagt ccgggcatat tcagagagaa catcaacatc
     1081 aagcggccac ccggctggtg gttccggttc gatggtatcg gttttgagca tatcgaagct
     1141 ttcaccgcca agttgaagga aattcggaag tgccacccgg tatacctggt catctttgag
     1201 tggtttgccc tggaccttga ccgattggat ccgtttccca accggggccc tgagatcaaa
     1261 ttcgtaactt aaccctgaaa ccagcaacag tcgcccgccg ctcatctcaa ctcgactggt
     1321 gccatgttca agtgcctgac gaagctgggc gccggtgtat ttcgccacga ccagccgatt
     1381 tccaaaaggg agcaggctaa cggcgttttt caacgtaaag actggtcctt cggcttcagc
     1441 ccggatgccg ccgctattga tgatgccgat ttcagttcca aagtgatggc gaaaggcgtc
     1501 agccacaaaa ttgccgagat tggtttcacg ccgtcggagg gcgttatcgg tgttgtagcc
     1561 agccagcagc ggtgttttga cctgagccag gggttcggcc agtttggtat caagttcggt
     1621 ttgataccgt tgttcaaact taagtaagtc gggggcactg gcaacggttt catcaattga
     1681 atggacccgg actgaaaaaa cacactttcc ctggttgagc gtgacatcaa gttgaactac
     1741 ggaaccggca tttccacagg gagacagcgc cgggcggttg tttacaaact gaatgatgct
     1801 ccggttttca aactgttctt caccaaaaat cgcatcaata tctggaactt gttcaagcat
     1861 ttgcaaattt ttgggcatat cagcctgaca tagggcgacg attacatccg ccccttgttt
     1921 gcgcaaggct gaggtggcgg atttggctga ttgcaaaata ttttgttgcg tgacgatgtt
     1981 ttccggcaag gtggtgtcaa aatcatcggt caatccaatg aacccaattt tgaggccgcc
     2041 tgattcacga atcagatacc gtgggacgcc ggcaaatggc tggcccgatt gttcaaccag
     2101 gttggaagta atccagttga attttgattc agcaatcagt ttacgagttt cagccgaccc
     2161 aaaatcaaaa tcgtgctgcc caaatgaggc tacatcaact ggaagttgat taaaggctgc
     2221 cacttgcgga tgtccatgaa aaacagcgcc aaaaagcagc ccacctgcca gatcgcctcc
     2281 aaacgcaacc agagtgctcg gattgtcgcg cttgacctga tcaatgatgg tttttaaccg
     2341 ggccacgccg ccccgaatac cgagttgatc gctgaccggc atcacctgat gggcgtcgtt
     2401 gaaaaacaaa attgaaacgg tttttgactt tgattgggga gcgatggggt gagtcggttt
     2461 ggcaaagagg ctgaagggtg aaaaaagcac aagcaacagc ccgcaaatgt aggtcaggtg
     2521 agtcatccgg tgtgtgatgt ccttattctt tcgatagctc ggagtctaat gccattttgg
     2581 gctgagggtg tcgggctaag ggaaatacaa tcctatcaat aatttaaact ctcactcatc
     2641 ctgatgggat tattccaaaa tggtctactc agtgagcttt atcccagagt agcgcggcaa
     2701 gcagtcgaaa atgtctgaaa tagacaaaac tttcaacccg ttctgccgct ttggaagagg
     2761 gctgaaggca ttgggctgag gaaggcgggc tcagggcgag gggctcaggg ctgaagaagg
     2821 cgggctgaag aatatcgggt tcagggtatt gaacccgttt tcttcagccc cgagcttgcg
     2881 agtcttcagc cctgagcccc tcgcccgctt ttttcagccc tgagcccctc gcccgctttt
     2941 ttcagcccga aaatgttcac cgccgggcga gtgggtcaat atacggtcga gattgcagaa
     3001 tataaacctg atcgccgacc agtgcccatt cgatatcctg ttctttctgg ttgaaaaggt
     3061 gtttgatctt gagcgcagcg gctgacaatc gccgggcatt ggcatcggtc agaatagccc
     3121 ggtctgagaa caattgaatt tctttaacgc ctccattttg atcaaacgtc agaaggctgt
     3181 cttcacctga tcgggagaga acccgcacgg cattggatcg agttgaaaaa ataatctgtt
     3241 ccggtatctt ctggccttca acaactttaa tccccaatcc gcgcttggca ttgatataga
     3301 ccccgccagt atcctgcgga tcaaacgggt tggtggtaat catcacgccg gcgctttcag
     3361 cgttgatgcc ggtttgtaac aacaccgccg gatacacggc gaggtgatca attttggcgg
     3421 cttcgcgggc ttcaaaggct tcgaagttcc agattgaagc ccagactgtt ttgacggctt
     3481 caatcagagc gtcgtcacct ttgacattgg gaaccgttgt atagagcccg gcgccattaa
     3541 agttgggtaa atcttcagca ttggttgaac tccgaacaaa cagcccggcg gtgggaaatt
     3601 ctcgatggac cttttccaaa atcgctattt ttaacgcctc tggcatgggt gatgtctgca
     3661 cataggcacg tagttcagcc agttttactt tgcggatttt tgggtcgtgg tgaaagtgct
     3721 cgtcgttgag caattccagg acgcgttctt ccaggccaat ctttttggca aattccacat
     3781 agcaggcaaa aggaatggta aatcctgggg ggacgatgac ccctttgatt ttggcctggg
     3841 cgacctgacc gagattggct gatttggcac caaagcgaac gacatcgcgg cttcgttgtt
     3901 gggccagatc ggtcaactga ttccagctca ggtcggcccg tggcacctgg agttgcagcc
     3961 gtttcgccac ctgtggactc agtcgcttgg cggcattgac atccacaggt tcaatgacaa
     4021 tttcgtcatt tctggctttg aggcggaccc atttgttaag caacgccttg tatttttcat
     4081 cggcatcttt gacaaagacg tttggaatgt tccacgtctt ggaaagtaaa ttgagatgag
     4141 acagcggtgt tccaggccgt gtggtgacaa tgcctgaaat cggcgtcacc gaaatcggaa
     4201 cctgcttgaa aatcacgatg tcttcgggtt cgacgggttg ttcatccggg agattttccc
     4261 gcagtcgcaa gatgccgacc gtttcgccgg gatggataat ttcctcagta ttaaagggct
     4321 ccagttcgga aatcaggatt cgggggacgg tgggcagttc gtcggaaagc tgctcttgcc
     4381 gcaacgaatt gggtttaaat ttgatcggcg caaaaaatga tttctggatc gtagaaaatg
     4441 tgtattcaac cagctctttc gggatcaggt cgccttccca aaattcaaac gtgaagagtt
     4501 tggcctcagt ttgataggcc agcgttccca gaataaatcg ccgattgggg tttaaataat
     4561 tcttctcaaa gaacttcgcg ccgcgctcaa gtgaccagta gttggaaaag acaaaatcgg
     4621 tgtgaaacgt gtagaggttt gaattcacat aggcaatcgt tttttcgttg cggtcaatca
     4681 caaacatcag gtgcggaatc gaagcaaatc gcccccgata gtaaatccgt ttcaacgtgt
     4741 caaattcaga ctgggttgaa atctggtgtc gaaaactggg cggttttttc ttttgaaggg
     4801 cagaagctgg aactaccagc aaacacagga gtaagaacga tccaatcgct tttaagatca
     4861 tagaatgaat atctttgtcc gttctgggaa tgagctggtt gatgaccgta aactacaaat
     4921 caaaacatag acttgtgaga aagagaagca ttaagagttg cgtctttaga cgagagggtt
     4981 ttctatttac tcagctccag
//
LOCUS       DAOQDO010000118         4992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10931_length_4992_cov_3.476403, whole genome shotgun sequence.
ACCESSION   DAOQDO010000118 DAOQDO010000000
VERSION     DAOQDO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10931_length_4992_cov_3.476403"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKZ53_02950"
     CDS             <1..261
                     /locus_tag="PKZ53_02950"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39421.1"
                     /translation="YDLQRQLRQADEVPSGDLIKARADLASLTEDERQLFGLIGLDEP
                     IHIDVLLVQSKLNQPRLVNALFQLELKDRIHQLPGKLYVRKL"
     gene            422..2926
                     /gene="topA"
                     /locus_tag="PKZ53_02955"
     CDS             422..2926
                     /gene="topA"
                     /locus_tag="PKZ53_02955"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNJ39422.1"
                     /translation="MSKSLVVVESPAKAKTINKYLGKTFTVMASNGHIKDLPDKGLGV
                     DVEHGFEPTYEVIPDTVKRNNSKTLRELKKAAKEAEIIYLAADPDREGEAICQHLREE
                     LAGARSKKPVYRLMFNEITQEAIQKAISVPRQIDSHRVDAQQARRVLDRLVGYQVSPM
                     LCRKVGGKLSAGRVQSVALRLVVERERVIQAFKPTEYWTFAAELKAKLPPQFTSSLHQ
                     ISGKTLKTGDFDNFKKTEVHIQNETDARAIETELKAVPEWKVASVTTKEKKRYPVPPF
                     ITSKLQQEAARKLGFAVKKTMQVAQSLYEGVDLGSEGSVGLITYMRTDSTRVSEGAIT
                     EVREFIQAQFGASSLTPQPVRYKTKKDAQDAHEAIRPTSVYRTPESVAKSLGKDELAL
                     YKLIWKRFVASQMMPAVFDATTIDIQAGPKFLFRATGSVLRTPGFLAVYEEGKDERDT
                     EDEELALKLPKVEIGEILNCLGVAANQSFTKPLPRFTEATLVKALEEEGIGRPSTYAQ
                     ILSVILSRAYVEKTEGRFRPTELGMVVNDVLVENFTDLFNINYTAAMEAQLDAIEEGS
                     LNWREALKSFYEKFRVELKSAESNINRAKAGVPTDEKCLKCESPMVLKLGRFGKFLSC
                     TNPECKATRDLETPATESAEPVENPYANETCDTCGKPMALKKGRWGEFLACTGYPECK
                     TTRQIRKSGVVRKPDVLLEELCPQCGKQLARKQGRFGEFVSCSAYPKCKYIKRDTLIA
                     CPRKGCKGEIAVKKSKRGKAFYGCTEYPKCEIVYWDRPIPTPCPKCKAAFLLEKTTKK
                     EGTILYCADKEGCGYVAPATAGAESPGTEPSLQATT"
     gene            3134..3877
                     /locus_tag="PKZ53_02960"
     CDS             3134..3877
                     /locus_tag="PKZ53_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ39423.1"
                     /translation="MTTPRYVTIDPIADGIACLVIGCEPREQGLTLEIFRELEAVVAG
                     LTSSEWKAVIITGKGTEVFVPGLDFAELAPLGPLEARRFAQRGQQVFGQLETLGIPVI
                     AAVNGVALGAGCELALACTVRVASTTARFGHPEVIQGKFPAFGATHRLTRLVGRGRAV
                     ELLLTGETIPAPEAYRIGLVNRVHEPARLLTAAKVLAQRLTRGSVKNVRYCLEALSHG
                     EEMSRQDGLSLEANLFGLCFLPDTPSDGD"
     gene            3964..>4992
                     /gene="purD"
                     /locus_tag="PKZ53_02965"
     CDS             3964..>4992
                     /gene="purD"
                     /locus_tag="PKZ53_02965"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNJ39424.1"
                     /translation="MKILVLGGGGREAAIVWKLLQSPRVSQVFCVPGNAGIAQHAKCI
                     EADLKRPEKLALLAQELKVDLTICGPELPLVYGAADMFARYNLPFVGPSRMAAQLEGS
                     KIFSKEFMSRHHIPTAMFSVCESVEAAEDILSNNFHGFPIVVKADGLAAGKGVVIAQD
                     LAEARAAVHMMMVDKKLGEAGSKIVLEECLVGRETSFLVFTDGRTIRPLPPAQDYKRA
                     YNGDAGPNTGGMGAFSAPGLLDEYLQGKLMRKIAEPTLAALQAEGILYKGVLYIGLML
                     TEAGPRVLEYNVRLGDPETQVLLPRLKTDLVEVCEAIVNSRLDSVDLQWSDDSVVTVV
                     MASGGYPNT"
BASE COUNT         1294 a         1203 c         1353 g         1142 t
ORIGIN      
        1 tatgatttgc agcgtcagtt gcgccaggct gatgaagtcc cttcgggtga tctgatcaag
       61 gctcgtgctg atctggcttc actcactgaa gacgaacgtc aactctttgg tctgattggg
      121 ttggatgaac cgattcacat tgatgttttg ttagtccaaa gtaagctcaa tcagccacgt
      181 ctcgtaaacg ccctgtttca actggaactc aaagatcgca tccatcaatt accagggaag
      241 ttgtatgtga gaaagctgtg atccgtgcgt ggtgactggt gcctggtgct tcggaaccaa
      301 tacttcgaag aatcaggaac gaaacacgaa gaacaaagca cgaagaacaa agcaccaaaa
      361 cccaagcacc aagaaccaag aaccaggaac caaaacccaa gaaccatttc aaggattgag
      421 catgtcaaaa tcgcttgttg ttgttgaatc accagctaaa gccaaaacga ttaataaata
      481 tcttggcaaa acgtttacgg tgatggcgtc taacggacat atcaaagatc tgcccgataa
      541 agggcttggc gttgatgtgg agcacggatt tgagcccacg tacgaagtca ttccggatac
      601 ggttaaacgc aataattcca aaacattgcg tgaactgaaa aaggctgcca aagaagctga
      661 aatcatttat ctggcagccg acccggatcg tgaaggggag gcgatttgtc agcatttgcg
      721 cgaagaactg gctggtgccc ggtcgaaaaa accggtctac cggctgatgt ttaacgaaat
      781 tacccaggaa gcaattcaga aggcgatttc agtgcccaga cagattgatt cgcaccgggt
      841 ggatgcccag caggcacgcc gggttttgga tcgtctggtc ggatatcaag tcagcccgat
      901 gttatgccgt aaagttgggg gcaagctctc ggctggtcgg gtgcaatcgg tggccttgcg
      961 actggtggtg gaacgcgagc gagtgattca ggcatttaaa ccaactgaat actggacgtt
     1021 tgcggctgaa ttgaaggcaa aactgccgcc gcaatttact tcatcgctcc atcaaatttc
     1081 aggaaaaacc ctcaaaaccg gtgatttcga caacttcaaa aagaccgaag ttcatatcca
     1141 aaatgaaacc gatgcacgcg caattgaaac cgaactcaaa gcggttccag aatggaaagt
     1201 ggcatcggtg acaaccaaag agaaaaagcg gtacccggtt ccgcccttta tcacgtcaaa
     1261 acttcagcag gaagccgccc gcaagcttgg gtttgccgtg aagaaaacca tgcaggtggc
     1321 acagtcgctc tatgaagggg ttgacctggg gagcgaaggc agcgtcgggt taatcaccta
     1381 tatgcgaacc gactcgacgc gtgtgtctga aggggcgatt actgaagtcc gcgagtttat
     1441 ccaggcacaa tttggtgctt cgtcgctgac cccgcaacca gtgcgctaca aaaccaaaaa
     1501 agatgcccag gatgctcacg aagccattcg cccaacttcg gtgtatcgga cgcctgaaag
     1561 tgtagccaaa tcattaggaa aagatgagtt agcactctat aaactcatct ggaagcggtt
     1621 tgttgcttcg caaatgatgc cggctgtctt tgatgccacc accattgata ttcaggctgg
     1681 gcccaagttt ttgtttcggg ctaccgggtc ggttctgcgt acgccaggtt ttcttgcagt
     1741 ttatgaagaa gggaaagacg agcgggatac agaagatgag gaactcgccc tcaaactgcc
     1801 caaggtggaa atcggtgaga ttctgaactg cctgggggtg gcagccaatc agagttttac
     1861 caaaccgctt ccccggttta ctgaggccac actggtgaaa gcgcttgagg aagaagggat
     1921 tggccgacca tcaacctatg cacaaatcct gtcagtgatt ctgagtcggg cttatgttga
     1981 aaaaacggaa ggccgtttcc gtccgactga acttgggatg gtggtcaacg atgttctggt
     2041 cgaaaatttt actgacctgt ttaacatcaa ttacaccgcc gccatggaag ctcaactgga
     2101 tgccatcgaa gaaggaagtt tgaactggcg ggaagcgctc aaatcctttt atgaaaaatt
     2161 cagagttgag ttgaaatcag ccgaaagcaa catcaatcga gccaaggccg gtgttcctac
     2221 cgacgaaaaa tgtctcaaat gcgaatcgcc gatggtgctc aagcttgggc gattcggaaa
     2281 atttctgtcc tgcaccaacc ctgaatgcaa agccactcgc gatcttgaaa ccccggcgac
     2341 tgaatccgct gaaccagtgg aaaacccgta tgccaacgag acctgtgata cctgcgggaa
     2401 acccatggcc ctcaaaaaag gccgctgggg tgaattctta gcctgcaccg ggtatccaga
     2461 gtgtaaaacc acccgccaga ttcgcaaatc aggcgtggtt cgcaaaccag acgtgctgct
     2521 cgaagaatta tgcccgcaat gtggcaagca acttgcccgg aaacagggcc gctttggcga
     2581 atttgtttcc tgctcggcct atccaaagtg caaatacatc aagcgtgaca ccttgattgc
     2641 ctgtccccga aaagggtgca agggcgaaat tgccgtcaag aaatccaagc gggggaaagc
     2701 cttttacgga tgtactgagt atcccaagtg tgaaattgtg tactgggatc ggccaatccc
     2761 aactccttgt ccaaagtgca aagccgcgtt tctacttgaa aaaacaacca aaaaagaagg
     2821 gacgattctg tattgtgctg ataaggaagg ctgtggatat gtggcaccag ccacggctgg
     2881 agctgaatca cccggaactg agccgtcatt acaagcgacg acataattct gagaactaag
     2941 tcaatcgtca gtagctgata gttgataagt cagtcgtcag tggaccaaac ccaggtattt
     3001 cagtctgcaa agagcttgaa agaactcttc gtaaatgact caaaaagaac caaatccatt
     3061 tgacacgaat tcccaaatcc caacactcga caactgacaa ctgacgactg actacggtac
     3121 aacctaaacg tcaatgacca caccacgtta cgtcacaatt gaccccattg ccgacgggat
     3181 tgcctgcctg gtgatcggct gtgaaccccg cgagcagggg ctgacccttg agatctttcg
     3241 tgaacttgaa gcggtggtcg ccgggcttac atccagtgag tggaaagcgg taattattac
     3301 cgggaaggga accgaagtgt ttgtgccggg cctggatttt gctgaactgg caccgcttgg
     3361 cccactcgaa gcccgacgct ttgcccagcg tggtcaacag gtgtttggac agcttgaaac
     3421 actgggtatc ccagtcattg cggcggtcaa tggtgtggct ttgggggctg ggtgtgaatt
     3481 ggcgctcgcc tgtacggtgc gagttgcttc gaccaccgcc cggtttggac atccggaagt
     3541 catccagggg aaatttccag cttttggcgc gacacaccgg ctgacccggc tcgttgggcg
     3601 cggacgggct gtcgaattgc tcttgactgg ggaaacaatc ccagcgcccg aagcctaccg
     3661 cattggactg gtcaatcggg tgcatgagcc tgcccgcttg ctcaccgcag ccaaggtcct
     3721 tgcccaacgc ttgacacgtg gttcggtcaa aaacgtccga tactgcctgg aagcgctttc
     3781 tcacggtgag gaaatgtcac ggcaggatgg cttgagtctg gaagccaatc tgtttgggct
     3841 gtgtttcctg ccggacacac cctcagatgg tgattgacag gcacgaaccg tcacgttcaa
     3901 tgttggaaaa acaacctcct gtcctcatct cataattctt catcttttag aaatgtaccg
     3961 tttatgaaaa ttcttgtact tggcggcggc ggacgcgaag cagcgattgt gtggaaactg
     4021 ttgcagagtc cacgtgtttc acaagtgttt tgtgtgcctg ggaacgctgg gattgcccag
     4081 catgccaaat gtattgaggc ggatttgaag cgacctgaga agctcgcctt gctggcccag
     4141 gaattaaagg ttgatctgac gatttgtggt ccagagttgc cgctggtgta cggggcagcc
     4201 gatatgtttg cccgctataa cttgcccttt gtcgggcctt cacggatggc ggctcaactg
     4261 gaaggaagca agatcttttc caaagaattt atgtcgcgcc atcacatccc gaccgccatg
     4321 ttttcggtct gtgaatcggt tgaagccgcg gaagatattc tgagcaataa ctttcatgga
     4381 ttcccaattg tggtcaaggc agatggtctg gcggctggaa aaggagtggt tattgcccag
     4441 gatctggctg aagccagagc ggctgtccat atgatgatgg ttgataaaaa actaggtgag
     4501 gccgggtcaa aaattgtatt ggaggaatgt ctggtcggtc gagagacttc gtttctggtc
     4561 tttaccgatg gtcggacgat tcgcccgctc ccaccagccc aggattataa gcgggcgtac
     4621 aatggcgatg cgggcccaaa cacgggcggg atgggggctt tttcagcgcc gggtctgctg
     4681 gatgaatatc tgcagggtaa attgatgcgc aagattgcgg aacccaccct ggcagccctt
     4741 caggcggaag gtattctcta taaaggggtt ctctatattg gactgatgct gaccgaagcc
     4801 ggaccacgcg tgctggaata caatgtgcgg ttgggagacc cggaaactca ggttttattg
     4861 ccgcgcttga aaacagatct ggttgaggtg tgtgaggcca tcgtcaatag ccggctcgat
     4921 agtgttgatc tccaatggtc agatgattca gttgtgactg tggtcatggc ttcgggtggt
     4981 tatcccaata cc
//
LOCUS       DAOQDO010000119         4979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10978_length_4979_cov_3.157189, whole genome shotgun sequence.
ACCESSION   DAOQDO010000119 DAOQDO010000000
VERSION     DAOQDO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10978_length_4979_cov_3.157189"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKZ53_02970"
     CDS             complement(<1..712)
                     /locus_tag="PKZ53_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNJ39425.1"
                     /translation="MHVKKWLMRLTTIMLCVLAVSFGVKYSWDVPLSPVVQAQNQEAS
                     ESGALILLDKDRKPVGQCALKHTAVSASLSGNIGRVTVEQTFINRRSNRLEAVYVFPL
                     PQMAAVDRMTMKIGERVIEGEILSRAEAARLFKQARKNGETAALLDQERPNVFTQSVT
                     NIPPGATVVISISYVETLKYEDGEYEFVFPMTVGERYIPGSSPTDSEPKGLAVTVPDA
                     ARVTPPLSIRPGHDISLKL"
     gene            complement(976..1878)
                     /locus_tag="PKZ53_02975"
     CDS             complement(976..1878)
                     /locus_tag="PKZ53_02975"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNJ39426.1"
                     /translation="MPQPHVITTLAEAQLLDKTALPVRREPSRILMCPPDYFEIKDVK
                     NAFMQGNIGTLDKALAVAQWDVLKTTFEQLGKPVVTIEPAPDLEDMVFSANQVLPGLD
                     EDGTPYVVISRMRYPSRQREIPFYREWFADHGYRILELEQPGVCFEGQGDAIWHPGRK
                     LLWGGHGQRTDFAVYPELSERLGIPILALELSTPEFYHLDTCFCALDEQSVLIYPQAF
                     TSEGLALIHRFFPLVIEAPSAEAHKTFACNAFAFDGRNVVIQWGATETVAELRRVGFH
                     VTEVDTSEFIKSGGSVFCMKMEVY"
     gene            complement(1999..3327)
                     /locus_tag="PKZ53_02980"
     CDS             complement(1999..3327)
                     /locus_tag="PKZ53_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNJ39427.1"
                     /translation="MNRKRVLILGAAGRDFHNFNTVFRNHNVCEVVAFTATQIPNIDG
                     RKYPAELAGEFYPQGIPIFPEEDLERLISRLKVDEAVFSYSDIAHQDVMHLAARVNAA
                     GADFRLLGTKATMIKSTKPVISVCAIRTGCGKSPASRRIVQLLLDAGLKVAVIRHPMP
                     YGDLVKQRVQRFTTLEDLAAHDCTIEEMEEYEPHLIKGVTVFAGVDYEGIIRRAETEA
                     DVILWDGGNNDFSFYVPDLEIVIADPHRPGHELTYFPGEVNLRRADVIILNKIDSATP
                     EGIALVRANIREVNPTAMVIDAAMPLTVEKPEAIRGKRVLVVEDGPTLTHGSMKYGAG
                     VLAAEKFGAREIIDPRPFVVGSLAETFQHYPGIGRLLPAMGYGADQMHDLADTINRID
                     CDLVLIATPINLSRVIDIKHPTCRIGYELQEIGYPTLADALNPLLKSMVR"
     gene            complement(3324..4070)
                     /locus_tag="PKZ53_02985"
     CDS             complement(3324..4070)
                     /locus_tag="PKZ53_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ39428.1"
                     /translation="MSLHTASTSRPRIGLALSGGAVRGLAHIGVLKVLAEFGIHPEII
                     VGTSVGSLIGAAFAAGKNWQGIAEMATSVFWPNLLNSNLLEQFCDSHLPATFADLEHP
                     FAAVVTSIVDHQTVVIREGRLATAINASCALPYVRRPVVRDGKLFEDGGTTCVLPSEI
                     CRQFGADFVIASDVWGYGWCLRVVGLDPVTSQVFPEHYRRALQSTDILIQPPIPVVGY
                     LPGRQAVQAMVSEGETAARQALLALQENQV"
     gene            4656..>4979
                     /locus_tag="PKZ53_02990"
     CDS             4656..>4979
                     /locus_tag="PKZ53_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNJ39429.1"
                     /translation="MDEQKLVIANIEDEMKRSYLDYAMSVIIGRALPDVRDGLKPVHR
                     RILWAMHELSNTYNKPYKKSARIVGSVIGQYHPHGDSAVYDSIVRMVQDFSLRIPLID
                     GQGNFG"
BASE COUNT         1240 a         1342 c         1214 g         1183 t
ORIGIN      
        1 gaagcttgag ggaaatatca tgacccggac gaattgacag tggcggagtg acacgagccg
       61 catcaggaac cgtaacggcc aaaccttttg gttctgaatc agttggcgac gagcccggaa
      121 tgtagcgttc gccaaccgtc attggaaaaa caaactcata ctcaccgtct tcatatttca
      181 aggtttcaac ataactgatt gaaataacaa cagttgcacc aggtggaata ttcgttaccg
      241 actgggtgaa aacgtttggt cgctcttgat caaggagtgc tgctgtttcg ccattcttac
      301 gagcctgttt gaaaagccgg gcggcttctg cgcgtgacag gatttcacct tcgatcacgc
      361 gctctccaat tttcatcgtc atccgatcaa cggcagccat ctgcggaagc gggaaaacat
      421 agacagcttc cagacgattg gagcgccgat tgatgaaggt ttgctcaacc gtcacccgtc
      481 caatattgcc actcaacgaa gcgctcacag ccgtgtgttt gagcgcacat tgcccgaccg
      541 gttttcggtc tttatccaga agaatcaacg ccccgctttc actggcttcc tggttttgag
      601 cctgaaccac aggcgaaagc ggtacgtccc aactgtattt gaccccaaaa ctcactgcca
      661 acacgcacag catgatggtg gtcagtcgca tcaaccattt tttgacgtgc atagacagac
      721 ctctcccacc cacacgacgg ccttgacgga tagacacaca tcctgcggtt gtttcttaaa
      781 gaattgtcct gattggattt gtgtactttg acaccagaac gagaggttgg ttcccatttt
      841 tttgggctga agacatcggg ctcagggctg aagacattgg gttgaagaaa accagctaaa
      901 atttctttct ttaatccgaa agtcttcagc cctgagccct acttcttcag cccgaaaatc
      961 ttcagcccca ttctctcaat acacttccat cttcatacaa aacacactgc cgcctgattt
     1021 gatgaattcg ctggtgtcca cttcggtgac gtgaaatccg acccgacgca gttcggccac
     1081 ggtttcggtt gctccccact gaatcaccac gttccggcca tcaaaagcaa aagcattaca
     1141 agcaaaggtt ttatgtgctt cagcagatgg ggcttcgatg accagcggga aaaagcgatg
     1201 aatcagtgcc agtccttcgc tggtgaaagc ctgtggatag attaaaacac tttgttcatc
     1261 gagcgcgcag aagcaggtat cgaggtggta aaattctggg gtgctcagtt ccagggcaag
     1321 aattgggatg ccaagtcgtt cagacaattc aggatagaca gcaaaatcag tgcgctggcc
     1381 gtgcccaccc cagagcaatt ttcgaccagg atgccagatg gcatcgccct gcccttcaaa
     1441 gcacacgcca ggctgctcaa gttccagaat gcgatagccg tgatcagcaa accactcgcg
     1501 ataaaaagga atttcccgtt gccgcgacgg atagcgcatc cgggaaatca ccacatacgg
     1561 cgtaccgtct tcatccagcc ccggaagcac ctgattggca gaaaagacca tatcttccaa
     1621 atctggggct ggttcaatgg tgacaaccgg ttttccaagt tgctcgaacg tcgttttcag
     1681 cacatcccac tgtgccaccg ccagagcttt atcaagtgtt ccaatattgc cttgcataaa
     1741 ggcgtttttg acatccttga tttcaaagta gtctggcggg cacatcaaaa tcctggatgg
     1801 ctcacgacgt acggggaggg cggttttgtc aagtaactga gcttcagcaa gtgtagtaat
     1861 gacgtgtggt tgcggcatag ggggagttga gagttgaggg ttcagggttc agggttcggg
     1921 atcttcaaat tttcgtcttt tttgtccgtt ttgtcacttt ggtcctttgg tgttttccag
     1981 aaccctgaac cctgaaccct atcgaaccat tgatttcaac agcgggttca aggcatccgc
     2041 cagggttgga tagccgattt cctggagttc atagccaatt cggcaggtcg gatgcttgat
     2101 gtcaatcacc cgcgaaagat tgatcggcgt ggcaatcagc accagatcgc agtcaattcg
     2161 gttgatggtg tcggccagat cgtgcatctg gtcggcccca tagcccattg ccggcaacag
     2221 tcggccaatc ccaggataat gctgaaacgt ctcagccaga ctgccaacca caaatgggcg
     2281 tgggtcaatg atttcccggg caccaaactt ttcagccgcc agcacgccag ctccgtattt
     2341 catcgaacca tgcgtcaagg ttggaccgtc ttcgaccacc aaaacccgtt taccacggat
     2401 cgcttctggc ttttcaaccg tcaatggcat cgcagcgtca atcaccatcg cggtcggatt
     2461 cacttcgcgg atattggcac gaaccagcgc aatgccttcc ggtgttgcag aatcaatctt
     2521 gttgagaatg atcacatccg cacgccgcag attgacctca cctggaaaat atgtcaactc
     2581 atggcctggg cgatgggggt cggcaatcac aatttccaga tccggaacat aaaatgagaa
     2641 gtcgttattg cctccgtccc acaaaataac atccgcttcg gtttcggccc gccggataat
     2701 cccttcataa tccacaccgg caaaaacggt cacacctttg atcagatggg gttcatattc
     2761 ttccatttct tcaatcgtgc agtcatgggc agccaaatct tcaagcgtgg taaagcgttg
     2821 aacccgctgt ttgaccaggt cgccatatgg catcgggtga cgaatcactg ccaccttcaa
     2881 accggcatcc agcaagagtt gcacaatccg ccgtgacgcc gggcttttcc cgcacccggt
     2941 tcgaatcgca cacaccgaaa tcaccggttt ggtggacttg atcatcgtgg ctttggtgcc
     3001 cagcaaccgg aaatcagcac cagccgcatt cacacgtgcg gccagatgca tgacatcctg
     3061 atgggcaatg tccgaataag aaaaaaccgc ctcatcaacc ttgaggcggc ttatcaatcg
     3121 ttcgagatcc tcttctggga aaattggaat gccttgtggg taaaattccc cagccagttc
     3181 cgccgggtat tttcgaccat caatgtttgg aatctgggtg gcggtgaagg caaccacttc
     3241 acacacgttg tggttgcgaa aaacagtgtt gaaattatga aaatcacgac cagcggcacc
     3301 taaaatcaga acgcgttttc gattcatacc tgattctcct gtaaagccag caatgcctga
     3361 cgcgcagcgg tttcgccttc ggaaaccatg gcctgaacgg cctgacgacc gggcagatac
     3421 ccaaccaccg gaattggcgg ctggatcaaa atgtcggtgg actgaagcgc ccgccgataa
     3481 tgttccggaa aaacctggct cgtcaccggg tcaagtccaa ccacgcgcaa acaccagcca
     3541 tatccccaga catcggacgc aatcacgaaa tcagccccga actgacggca tatctctgac
     3601 ggcagcacgc acgtggtacc gccatcttca aacaattttc cgtcccgcac caccggacga
     3661 cggacatacg gcaaggcaca actggcattg atcgctgtgg ccagccggcc ttcgcgaata
     3721 accaccgtct ggtgatccac aattgacgtc acgacggcgg caaacgggtg ctcaagatcg
     3781 gcaaaggttg ccggaaggtg cgaatcgcaa aactgttcga gcagattgct attgaggagg
     3841 tttggccaga aaaccgatgt ggccatttcc gcaatgccct gccagttttt tcctgctgca
     3901 aatgctgcgc caatcaaact gccgacactg gtgccgacaa taatttctgg atggatgcca
     3961 aactcggcca ggacttttaa aacgccaatg tgtgccagac ctcgcacggc gcctccactg
     4021 agggccaaac cgatgcgcgg acgagaagtt gaagcggtgt gtagtgacat aagatttggg
     4081 ctgagggctt ggggctgagg gctcggggtt taaattcaga tctttgttgt ctttgctgtt
     4141 cttttcgttc ttttccggcc tttacagtct ttgaattatg acactttggc aattgtgttt
     4201 atcgcgcgga gaaagcctca aaaaacacct tcgaaaatac gatgtaactc aatcatagac
     4261 aaggggtttg tcgtttgcaa aacctgtcgg tttgagatat agtgccgcca ttctaaaaac
     4321 cgcttgagaa atccgcttca aaccgctctt aacccttcat tcctaagaag ttaggtggtt
     4381 tccctgcatt cagtgtggag atttgcttga agacctgata caattactca gttatagaca
     4441 gggtacaacg tctcacactg tgctcccctg ttgaccaacc agccagacag ctccctgcac
     4501 gctcccagtt gcctttcaaa aactgatggg ctggcctgag atttggctgc attctctgac
     4561 ggatacctga accaacccac ctccgtgact ggtaacaggg cacaacaaaa attcagtttt
     4621 tctaacctca gttgattttc atttggagta caagcatgga tgagcagaag ttagtcatcg
     4681 caaatatcga agacgagatg aaacgtagct acctggacta cgcgatgtcg gtcatcatcg
     4741 ggcgcgcgct gcctgatgtc cgcgacggcc tcaaaccggt ccatcgccgt atcctgtggg
     4801 caatgcacga gttaagcaat acatacaaca agccgtacaa aaagagcgcc cgtatcgtcg
     4861 gatcggtcat cggtcagtac catccccacg gagactccgc cgtctatgat tccatcgtgc
     4921 gcatggtgca ggatttttcc ctgcgcattc cgttgattga tggtcagggg aattttggc
//
LOCUS       DAOQDO010000120         4976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10988_length_4976_cov_3.338346, whole genome shotgun sequence.
ACCESSION   DAOQDO010000120 DAOQDO010000000
VERSION     DAOQDO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10988_length_4976_cov_3.338346"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKZ53_02995"
     CDS             complement(<1..364)
                     /locus_tag="PKZ53_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ39430.1"
                     /translation="MTTGSFYCPRCGHQQASESQKFCRNCGLQVGSIHKLLQTDSQEE
                     AELILNEDPLRQRDINLGSVLMLVVVIKAIAFLEFPPLVAVDPYFFSLAFLSLGLGIL
                     LLLSQFTHRRRGLTVGATL"
     gene            complement(550..3564)
                     /locus_tag="PKZ53_03000"
     CDS             complement(550..3564)
                     /locus_tag="PKZ53_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39431.1"
                     /translation="MGSTKIVSLPPSLESFVETLPDEAAARRFAERLVDEHPMLAERL
                     WQRPEVLTWILTLSSYSSWLGETLLQRPEFLSWLEREPHLNEAKSRDDLSQELARMAV
                     MQSTLEPSVLLSRFKRREWVRIYLRDCLNRCSLSEVTEELSHVADVILGYALERCRST
                     LISRYGTPMIRDERGRSSPATFAVLSLGKLGSHELNYSSDIDLLFLYSGQGETEGGGE
                     GSLRNREFFTRLAENLLRMVGSLTAPEGAAFRIDVRLRPRGRDGALVVPTNEATSYYR
                     NEAQNWERLAMLRARGSAGDIQLAQDFLDSIQESIYLAEPLEVALRDVRLSKEKIDRK
                     QAERSSGYNVKLGKGGIREIEFIAQALMVCHGGKDTWLRSPQTLIALQRLAEKGHLSE
                     TERSQLAEAYTFLRTCEHRIQMEQGAQTHTIPLDEEKLGLLARRMGYQGPYASRMFAL
                     ALARHRDHVTAIYQRIFADISPESAAPPATTTRTIATTPEEALRQAILKTFAMPNSGI
                     AQTEQSLWISLLESISARSCDPTRVLTLLRNMVASWAAYVRDIRSKINTASSLAEAGP
                     RSGFTTRLVHHKDEFKPWETDMRKHLLPDQTEVLTTAALAEAASFCEAGEFFAQHLTR
                     HPKLLPLLGKPPVLASFSRETLAAQFEAALANCVTLSEANAALRQIHSVALLEIGRAD
                     VISGAPLRVINLAQTALAEAAIEAACQFAGQYVARTCEFVSPMPTWSILGLGRLGHQG
                     IDYGSDLDLIVVYDETAPTPLPGLTNCQAVVKMVETVSSLLTALTADGMLYRVDFRLR
                     PDGRNGPLAVSKSGLLNYARGRAASWEHLAYLKMRPVAGNLEFGTELQQAAREVIFEY
                     ARQRGDVLRHEVAEIRSRLQSERGGRSPERDFKFGIGAMMDVYFITRYVQMRDGIPDP
                     PEYGTIALIDNLITRQAIRPEAGRILRDGYNFLRTLDHAMRLLVERHTSRLPAQTELL
                     ARILKYRGASAFEEDYQRHTKAIRTVYLETFS"
     gene            complement(4012..4404)
                     /locus_tag="PKZ53_03005"
     CDS             complement(4012..4404)
                     /locus_tag="PKZ53_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ39432.1"
                     /translation="MSRQNISSGTKWEPIVGYSRAVRVGSVIHVSGTTATAPEGGLVG
                     IGDPYAQTVQTIRNIERALQAAGATLNDVVRTRIYVINISEHWEQVGKAHGEFFGDIR
                     PATSMLEISRLIDPEMLVEIEAEAIVSS"
     gene            complement(4518..>4976)
                     /locus_tag="PKZ53_03010"
     CDS             complement(4518..>4976)
                     /locus_tag="PKZ53_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ39433.1"
                     /translation="PLSATIARADLMNWHVGAHASTFGGNPVAIAASLKTFELLNNGL
                     LANANAMGQRMMDGLRKLQEKYSTIVADVRGLGLMIGIEFVKDLTSMTPWAALRNQIE
                     TECFNRGLILLGAGESTIRFSPPLVIDEDQVDCALSILDEAIAASLKVVS"
BASE COUNT         1204 a         1299 c         1364 g         1109 t
ORIGIN      
        1 cgagggtggc gccgacggtg agcccacgtc gtcggtgcgt aaactgagac agcaaaagca
       61 atatccccag tccgagtgac agaaatgcca ggctgaaaaa gtaaggatca actgccacaa
      121 gcgggggaaa ttccaaaaat gcaatggctt tgatgacaac aaccagcatc aggaccgagc
      181 ccagattgat atcgcgctgg cgcaatggat cttcgttgag tatcagttcc gcttcttctt
      241 gcgagtcggt ctgcaacaat ttgtggatgc tacccacctg caacccgcag tttcgacaga
      301 acttttggga ttcagatgcc tgctggtgac cacaccgggg acaatagaaa gaaccagttg
      361 tcatagcgct acctgggggt tcagggtttc agggaggtgc caccagttga ttctgaatct
      421 gacagcctga tcatgtgaat atcttcggag gaagataaaa gaataaagaa tgaagcgtca
      481 aaaaggcaga aataaagctg aaattttttt actttttgca gtctaaatgc tttattccac
      541 gcacaatgct caggaaaacg tctccaggta aacggtccgg atggctttcg tgtgccgttg
      601 ataatcttct tcaaaggctg aggcacctcg atatttgaga atccgcgcca gcaattctgt
      661 ctgggctggc aggcgggagg tgtgacgttc gaccagcagc cgcatggcgt ggtcgagggt
      721 tcgtaaaaag ttatacccgt cgcgtaagat ccgaccagct tcgggtcgaa ttgcctgacg
      781 tgtgatcaga ttgtcaatca aggcaatcgt cccatattcc gggggatctg gaatgccatc
      841 gcgcatttgc acatagcgag tgatgaaata gacatccatc atggcgccaa tgccaaattt
      901 gaaatcgcgt tcaggtgacc gaccacctcg ctcagattgc aaccggctgc ggatttcagc
      961 gacttcatgg cgcaaaacgt ctccgcgttg gcgggcatac tcaaaaatga cctcgcgggc
     1021 tgcctgttga agttcggttc caaactccag gttgccagcc actggccgca ttttcaaata
     1081 cgccagatgt tcccaactgg cggctcttcc acgggcataa ttcaacaaac cgcttttgct
     1141 caccgccagc ggaccatttc gtccatcggg acgcaatcga aaatcaaccc gatagagcat
     1201 tccatcagcg gtcagggccg tcagcaaact ggagacggtt tcgaccattt ttaccacggc
     1261 ctgacagttg gtgagacctg gcaaaggggt tggtgccgtt tcgtcataca ccacaatcaa
     1321 atccagatcg gaaccataat caatgccctg gtgtccgagc cggcccaacc caagaattga
     1381 ccaggtcggc attggggaca caaactcaca ggtgcgggcc acatactgac cggcaaactg
     1441 acaggcggct tcaatcgcgg cttcggccag tgcggtttgg gccaggttga taacccggag
     1501 gggggctcct gaaatgacat ccgcgcgccc aatctcaagc aacgccaccg aatgtatctg
     1561 acgcaacgcc gcgttggctt cactcagggt gacacagttt gccagggcag cttcaaactg
     1621 ggccgccagc gtctcgcgcg aaaagctggc caggaccggt ggtttcccca gcaatggaag
     1681 caattttgga tgacgcgtca aatgctgggc aaaaaactcg ccagcttcac aaaaagatgc
     1741 tgcttcagcc agggcggctg tggtcagtac ttcagtctgg tccggtagca ggtgtttccg
     1801 catatcggtt tcccagggct tgaactcatc tttgtggtgg accagtcgag tggtaaaccc
     1861 ggaccgtggg cccgcttcag ccagagacga tgcggtgttg attttagagc ggatatcgcg
     1921 cacataggcg gcccacgacg caaccatatt tcgcaacaag gtgagcacgc gggtggggtc
     1981 acatgaccgg gctgaaatac tttcgagcag tgaaatccac aaactttgtt cggtttgcgc
     2041 aattccggaa ttgggcattg caaacgtttt gaggattgcc tgtcgcaggg cttcttccgg
     2101 tgtcgtggcg atggtgcggg tggtggttgc cggtggggcc gccgattcag gtgaaatgtc
     2161 agcaaaaatg cgttgataaa tcgcggtgac atggtcgcga tgacgggcca gggccagcgc
     2221 aaacatgcgt gaggcatagg gcccctgata acccatccgc cgcgccagca accctaattt
     2281 ctcttcatcg agcgggatgg tatgggtctg agcgccttgt tccatctgga tgcgatgttc
     2341 acaggtacgc agaaacgtat aagcttcggc aagttgggac cgttcagttt cggaaagatg
     2401 gcctttttca gccagccgtt ggagggcaat caacgtttgg ggtgagcgga gccaggtatc
     2461 tttgcctccg tggcacacca tcaacgcctg ggcaataaat tcaatttcgc ggatgccgcc
     2521 ttttccaagt ttgacattat agccgctgct gcgttcagcc tgcttgcggt caattttttc
     2581 tttggacagt cggacatccc gcaaggcgac ttcgagtggt tcggccagat aaattgactc
     2641 ctggatcgaa tccagaaaat cttgagcaag ctggatatcg cccgccgaac cacgtgcccg
     2701 gagcattgcc agacgttccc agttttgggc ttcattccga tagtaactgg tggcttcatt
     2761 cgtcggaacc acaagtgctc cgtcgcgtcc gcgtgggcgt aaccgaacgt caattcggaa
     2821 agcagcgcct tccggcgccg ttaatgaccc caccattcgg agcaggtttt cagccagccg
     2881 ggtgaaaaat tcacggttgc gcaatgaccc ttcgccccca ccctcagttt caccctgtcc
     2941 cgagtataaa aacaacaaat caatgtctga cgaatagttc aattcgtggc tgccaagttt
     3001 acctagtgac agaacggcaa acgtcgctgg tgaacttcgt ccgcgttcat cccgaatcat
     3061 cggtgttcca tagcgggaaa tcagcgttga gcgacaacgt tcaagcgcat aacccagaat
     3121 gacatctgcc acatgagaaa gttcttccgt tacttctgaa agcgagcagc ggttcaggca
     3181 gtcacgcaaa taaatccgga cccactcacg ccgtttaaac cgcgacaaca aaaccgaagg
     3241 ttcgagcgtg gactgcatca ccgccatgcg ggccagttcc tgcgacaggt catcacgtga
     3301 tttggcttcg tttaaatggg gttcgcgttc gagccaggaa agaaattccg gacgctggag
     3361 cagtgtttca ccaagccatg aactatagga actcaacgtc agaatccagg ttaacacttc
     3421 ggggcgttgc cagaggcgtt cagccagcat tggatgttca tcaacgagcc gctctgcaaa
     3481 tcttcgggca gcggcttcat caggcaacgt ttcaacaaaa gactctaagg agggggggag
     3541 ggacacgatc ttggtagagc ccattgtgac tacctcaacc agttttgaaa attgtatcaa
     3601 ctgtgctttg gagtggggca aaatcgaaca ccaacaatac ctgaatgcag aaaagaacgg
     3661 tagccggatg accgaaaaaa ccagggctga gggcttgggg ctgaagaata tcgggctgaa
     3721 gaaaatgggc tgaagaatat cgggctgaag aaaatgggct gaagaaggcg ggctggaaaa
     3781 accagggctg atgggacaag gagagaattc caccgacgtg gtgagtgtga atgacctccg
     3841 gttaggagcg gggaacgacg accatctctt tgtgtcaccg tgtcaccctg tcaccgtgtc
     3901 cagatcgggt ttatctcgct tatcctggcg cttatgggcc cggagcctgt cttcttcagc
     3961 ccgatgtctt cagcccgatg tcttcagccc gacttctcca gctctcaggc ctcagctcga
     4021 aacaatggct tcggcttcga tttcaaccag catttccgga tcaatcaaac gactgatctc
     4081 aagcatgctg gtggcaggtc gaatgtcacc gaaaaattca ccgtgggctt ttccaacctg
     4141 ttcccagtgc tcactgatat tgatgacata gatccgggtc cgcaccacat cgttgagcgt
     4201 ggcaccggct gcctggaggg cgcgttcaat gttgcggatg gtttgaactg tttgagcgta
     4261 gggatcacca atgccgacca gtccaccttc gggagccgtc gccgtggtgc cggaaacatg
     4321 gatgactgag ccaacccgaa cggctcgtga atagccaacg attggttccc atttcgtacc
     4381 gctggaaatg ttttgtcggg acatatgaat cttcaaaaaa aggtacagag ttcaagcttg
     4441 agtttgctcc cactcaatgg atcaaccaca gcttgaactc tgtaccgaaa tcaggttctg
     4501 actacttgcg atgatggcta gctgactact ttgagcgaag ccgcaatggc ttcgtcaagg
     4561 attgacaggg cacagtcaac ctggtcttcg tcaatcacca gaggtggtga gaaacggatg
     4621 gttgattcgc ctgcgccgag caggatcaat ccccggttga agcattcggt ttcaatctgg
     4681 ttgcgcaatg cggcccacgg cgtcattgaa gtgaggtctt tgacaaattc aatgccgatc
     4741 atcaacccga ggccgcgcac gtcggccacg atggtggaat atttttcctg gagcttgcgc
     4801 aggccatcca tcatccgttg ccccatggca ttggcattgg ccagcaatcc attgttgagc
     4861 aattcaaagg ttttgagcga agcggcaatg gccaccggat tgccgccgaa ggttgaggcg
     4921 tgggcgccga cgtgccagtt catcaaatca gcccgggcaa tcgtggcgct caacgg
//
LOCUS       DAOQDO010000121         4976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_10989_length_4976_cov_3.336923, whole genome shotgun sequence.
ACCESSION   DAOQDO010000121 DAOQDO010000000
VERSION     DAOQDO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10989_length_4976_cov_3.336923"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKZ53_03015"
     CDS             complement(<1..1022)
                     /locus_tag="PKZ53_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39434.1"
                     /translation="MSRFNSPGRSLFRLALMHQPDEKPKMPPSTQCHTAFTKLLKDLS
                     KIPASQKMKYSGYGLPPVYKYSLSQKEVELLPELYRKVLERGGNGANRVLENLLEFLV
                     RVPGTNHFPFLLEALSLARPRDKGLPQRQEIILSYLSIAAVQRKDQVAFQHLMDLTRH
                     ESVDIRAQALEHLSVTYSILDQPVPQSVIDRAQVMVVEESEFYPRFLARQILQEANLP
                     TPLENQGGTYSFKVHHRYLGKGVYRIIEVKSEQTLADLHLAIQSAFEWDNDHLYAFHL
                     DGDSRNESFEFQGSPVMFDNPFGADAPPANEAILGKLGLSLHHKFLYFFDFGDSHEFD
                     VEVVAIT"
     gene            complement(1055..4060)
                     /locus_tag="PKZ53_03020"
     CDS             complement(1055..4060)
                     /locus_tag="PKZ53_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39435.1"
                     /translation="MTCRVSWFWFARLFLSFLLPCVVSAFNPVTALDPAKRVTQYGHD
                     AWQTEQGLPQNSVTAILQTRDGYLWLGTQEGLVRFDGVKFTVFDKSNTPAFTNHFILS
                     LCEDRLGRLWIGTYGGGLVCLDQGKFSTYTRQDGLSNEVIRVLCEDQQGNVWIGTDRG
                     LNRLTNGRFKVFISPTGSFDERISAVAQDRDGNLWVGFTSGVLTFLHNDQLRFFASTE
                     TFTDASIRSIYSDQARRLWVGTDGKGLFCFAGDEVTTYTTHNGLSDDSVIVVYEDRRG
                     NLWIGTGNGLNRLSSGQLTTYSSGEGLTDERVLALAEDSEGSLWIGTDGGGLNRFCDT
                     KFTMFSTAEGLSNSDIISLCEDNQNTLWIATSGGGVNCLNDGKITTTYTTRDGLSSND
                     VSSICATIDGGVWVGTSGGGLNYFKDGKVQSHLSTANGLTSNFIWSIFQAHDTTLWVG
                     TNNGLNHIENGRITRFTQQEGLSNNVVWTICEDSDHNLWVGTDGGGLNQFKDGKIRVY
                     TTQDGLSNNFVVSLYRDTQGSLWIGTAGGGLNQYHNGKFTAFTSRDGLFDDTVYQILE
                     DNQNCFWMSSNKGIFRIQKSQLEQFARQELKLLECTVFSKADGLRSSECNGGVTPAGW
                     KTHDGKLWFPTIKGVAMIDPAQIQLNVQVIPVRIEQVLVDSQPVSPLDQITFKAGVQK
                     VEFHFTGLSYRVPSRVKFRFKLDGYDPDWTTQAGPRVAYYTRIPAGEYTFQVVACNDD
                     GVWNTTGTSVKLTVKPHFYQTYWFYGLCILGVGVTGGLLYSLRVAQLKRRQRILEELV
                     EKRTWQLEKANQELQRLSYVDALTGVANRRHFDEVFDLEWRRAIRAKTPISVILIDVD
                     SFKLFNDTYGHQGGDECLRRIGQVLKETVIRAGDVVARYGGEEFVAVLPGSSTESAWN
                     LAEILRTRTESLQIPHEKSLAASKMVTLSQGIATVVPRLDLSPESLIQAADQALYQAK
                     HEGRNRVRVAQTVPVPDHSTNEPSRIQAE"
     gene            complement(4125..4198)
                     /locus_tag="PKZ53_03025"
     tRNA            complement(4125..4198)
                     /locus_tag="PKZ53_03025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4162..4164),aa:Met,seq:cat)
     gene            4401..>4976
                     /locus_tag="PKZ53_03030"
     CDS             4401..>4976
                     /locus_tag="PKZ53_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNJ39436.1"
                     /translation="MEAVLALEDGRVFRGRSFGAVGETAGEVVFNTSMTGYQELLTDP
                     SYAGQLVVMTYPHIGNYGINREDMESRRPFLQALIVKELSPVVSNWRATETLDEYLRR
                     TGILGIEGIDTRALVRHLRERGAMRGVLSTTETNAETLVQRAASSPGMVGRALACEVS
                     CTKSYEWKNSHLFGEEPLVPAPDGEPFQVVAF"
BASE COUNT         1200 a         1281 c         1286 g         1209 t
ORIGIN      
        1 gtgatggcca ccacctcgac atcgaactcg tggctatcac cgaagtcaaa gaaatagaga
       61 aacttgtgat gaagcgatag cccaagtttg cccaggattg cttcattcgc cggtggggca
      121 tctgccccaa agggattatc aaacatcact ggtgaccctt gaaattcaaa cgactcgttt
      181 cttgaatcgc catccaggtg aaacgcatac agatggtcgt tatcccattc aaaggctgac
      241 tggatcgcaa gatgaagatc cgccagtgtt tgttcggatt tgacctcaat gatccgatag
      301 acgcccttgc caagataccg atgatggact ttgaaactat aggtgccccc ttgattttca
      361 agcggggtgg gcaggttggc ttcctgcagg atctgacggg ccagaaatcg tgggtaaaac
      421 tctgactctt ccacgaccat gacctgggcc cggtcaatca ccgattgtgg gactggctga
      481 tccaaaatcg agtatgtgac tgacaaatgt tcgagcgcct gagcccgaat atcaaccgat
      541 tcatgcctgg tgagatccat cagatgttga aaggctacct ggtctttgcg ttggaccgcc
      601 gcgattgaca ggtaggaaag gattatttcc tggcgttggg gcaggccttt gtctcggggg
      661 cgtgccaggc tcagtgcttc aagcaaaaat ggaaaatgat tggtgccagg tactcttacc
      721 aggaattcca ggagattttc caatacccga ttggcgccat tgcctccgcg ttcaagtact
      781 ttccggtaca actcaggcag taactctacc tctttttgag acagagaata tttgtagaca
      841 ggagggagtc cgtatccaga atacttcatt ttttgagacg ctggtatttt cgacaggtct
      901 ttcaggagtt tggtaaaggc agtatggcac tgggtgcttg gcggcatttt cggcttctca
      961 tcaggttggt gcatgagtgc cagtcgaaac agcgatctgc ctggggaatt gaatctactc
     1021 ataggggtta tgtgctttcc tcatgaacag tagatcactc ggcctgaatc ctggatggtt
     1081 cgttagtgga gtgatctggt acgggtacgg tttgggcaac tcggacgcgg ttgcgacctt
     1141 cgtgtttggc ttgatagagc gcctgatctg cagcttgaat caatgattcg ggcgaaaggt
     1201 ccagccgtgg caccacggtg gcaatgccct ggctgagagt caccatttta ctcgctgcaa
     1261 gcgatttttc atgtggaatt tgaagtgatt cggtccgggt tcgtaaaatt tcagccagat
     1321 tccaggcact ctcagtcgaa ctgccaggta aaaccgccac aaactcctca ccgccatagc
     1381 gtgccaccac atctccggct cggataactg tctctttgag tacctgacca attctgcgaa
     1441 gacattcgtc accgccctga tgaccatagg tgtcgttaaa cagtttaaaa ctatcaacat
     1501 caatcaaaat gacggaaatc ggtgttttgg cgcgaattgc ccgccgccac tccagatcaa
     1561 acacttcatc gaaatgccga cggttggcca cacccgtcag ggcgtcaaca taggacaggc
     1621 gttggagttc ctgattggcc ttttcaagtt gccaggtgcg tttttcaacc agttcttcca
     1681 ggatgcgttg ccgacgtttg agttgggcaa cccgcaggct atagagcagc cctcccgtga
     1741 ctcctacgcc aagaatacac aacccataaa accagtatgt ctggtagaaa tgaggtttga
     1801 cggtgagttt gaccgaggtg ccagtggtat tccacacgcc gtcatcatta caggccacaa
     1861 cttgaaaggt atattcgcca gccgggatac gggtgtaata agcgactcgc ggtccagctt
     1921 gagttgtcca atccggatcg tacccatcaa gtttaaatcg gaatttgacc cggctgggca
     1981 cgcgatagct cagtccagtg aaatgaaatt cgactttttg gaccccagct ttgaaggtaa
     2041 tctggtcaag agggctcacc ggttgtgaat caaccaggac ctgttcaata cgaactggaa
     2101 tgacctgcac attgagttga atttgtgccg gatcaatcat ggcgacgcct ttgatggtcg
     2161 gaaaccataa cttgccatcg tgcgtcttcc atccggctgg tgtcaccccg ccattacact
     2221 cactgctgcg caatccatca gctttgctaa acacagtaca ttcaagcagt ttcagttcct
     2281 gcctggcaaa ttgctcaagc tggcttttct ggatccggaa aattccttta ttgctgctca
     2341 tccagaagca attttgattg tcttccagga tttgatacac cgtgtcgtca aaaaggccat
     2401 cccggctggt aaaggccgta aactttccgt tgtgatactg atttaacccg ccacccgcgg
     2461 ttccaatcca caaactgccc tgtgtgtcac gatagagcga gacgacaaaa ttatttgaaa
     2521 gtccgtcttg agttgtgtaa acccggattt tcccgtcttt gaattgattg agcccaccac
     2581 cgtcagttcc aacccagaga ttgtggtcac tgtcttcaca gatagtccag accacgttgt
     2641 tggagagccc ttcctgctgg gtaaagcggg taatgcgccc attttcaata tggtttaacc
     2701 cattattggt gccgacccag agcgttgtgt cgtgagcctg aaaaatcgac cagataaaat
     2761 tgctggtcaa tccattggca gtgctcaggt ggctttgaac tttgccatct ttaaagtagt
     2821 tgagaccgcc tccgctggtg ccaacccaca cccctccatc tatggtggca caaattgaag
     2881 aaacgtcatt gcttgaaagc ccatcgcgtg tggtataggt ggtcgtgatc ttcccatcgt
     2941 tgaggcaatt gacaccgccg ccgctggtgg cgatccagag tgtgttctgg ttgtcttcgc
     3001 acagtgagat gatgtcactg ttggacagtc cttccgccgt ggaaaacatg gtgaatttgg
     3061 tatcacaaaa ccgattgagt ccaccgccat cagttccaat ccagaggctg ccttcggaat
     3121 cttccgcgag tgccaggacc cgttcatccg tcagtccctc gccactgctg taggttgtta
     3181 actgaccaga actcagtcgg ttaaggccat ttcctgtgcc aatccagaga ttgccgcgtc
     3241 ggtcttcata caccacaatc actgaatcat ccgagagccc gttgtgagtg gtataggtgg
     3301 tgacttcgtc tccggcaaaa caaaacagcc ctttcccatc cgttcccacc cacaaccgac
     3361 gggcctgatc actgtagatg gagcgaatcg aggcatctgt aaacgtttcc gttgacgcaa
     3421 aaaaccgcaa ctggtcattg tgcaaaaacg tcagcacgcc actggtaaag ccgacccata
     3481 gattgccgtc gcggtcctgg gcaacggcac tgatgcgttc atcaaagctg cccgttggag
     3541 agataaacac cttaaaccgg ccattggtca gacggttgag cccccggtca gttccgatcc
     3601 atacatttcc ctgttgatct tcacacagca cacggatgac ttcgtttgag agtccgtctt
     3661 gacgggtata ggtcgaaaat tttccctggt caaggcacac caggccgccc ccataggtgc
     3721 caatccacag ccgaccaagg cggtcttcac atagcgagag aataaaatgg ttggtgaaag
     3781 cgggtgtgtt ggatttatca aaaaccgtga acttgacccc atcaaagcga acgagtcctt
     3841 cctgcgtccc aagccacaga tagccgtcgc gagtctgcaa aatggcggtg actgaatttt
     3901 gagggaggcc ctgttccgtt tgccacgcat catggccata ctgggtgaca cgtttggccg
     3961 ggtcgagcgc cgtgaccgga ttaaaggccg aaacgacaca cggcagcaag aaggaaagaa
     4021 acaagcgtgc gaaccagaac cacgaaacgc ggcaggtcat gacagtttct ttgaagcgtg
     4081 aggtgaacgt gtggggatga agaaccggac tgaaggctgg aaaatagcgg cggttggact
     4141 tgaaccaacg acctacgggt tatgaatccg tcgctctgac cacctgagct acgccgccat
     4201 aaggattttc aaagtaaaag agcatacgga cctgagctga atctgtcaag catctgctga
     4261 agaccggtga tagttgtgat atggtagcag cgctcaaact ggcattcgcc cagttttcaa
     4321 aagctgagtc gctcaaaatc agaagttggc ggtcagcttt tcattcaacc aaatactttt
     4381 caactcagaa ggagtctctt gtggaagcgg tactggctct tgaagatggt cgtgtatttc
     4441 gtgggcgcag ttttggcgcc gtcggcgaaa ccgccgggga agtagtattt aacacatcca
     4501 tgacaggcta tcaggaactg ttgacagatc cgtcatatgc cggtcagttg gtggtgatga
     4561 cctatcccca catcggcaat tacggcatta accgggaaga tatggaatcg cgccgcccat
     4621 ttctccaggc gttgattgtc aaggaactct ccccggttgt gagcaactgg cgggcgactg
     4681 aaacgctgga cgaatacctg cgccggacgg gcatccttgg catcgaaggg attgacaccc
     4741 gggcgctggt tcggcatctg cgtgaacgcg gtgccatgcg cggcgtgctt tccaccaccg
     4801 aaaccaatgc cgaaacgctg gttcaacgag cggccagttc gccaggtatg gtcggtcgag
     4861 ccctggcctg cgaagtctcg tgcaccaaat cctatgagtg gaaaaactcg cacctctttg
     4921 gcgaagaacc tctggttcca gctccagacg gtgaaccatt tcaggtcgtg gctttt
//
LOCUS       DAOQDO010000122         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11108_length_4945_cov_3.375460, whole genome shotgun sequence.
ACCESSION   DAOQDO010000122 DAOQDO010000000
VERSION     DAOQDO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_4945_cov_3.375460"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKZ53_03035"
     CDS             complement(<1..398)
                     /locus_tag="PKZ53_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNJ39437.1"
                     /translation="MFRTLILNPELRTLNPEPRFRRRQTQQGFSLIEVLAVILIIGVI
                     AGLIGYFSGNAGPNANRELIALERIAQAVEEARQNARLGVEDTASRTVNFQSLDLSKR
                     VRLVTEVSTNSLPANTPVLNINDPLVFEVQS"
     gene            complement(422..1615)
                     /locus_tag="PKZ53_03040"
     CDS             complement(422..1615)
                     /locus_tag="PKZ53_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ39438.1"
                     /translation="MARHFHSTQNTYRQAGFSFMELLIGLLIFSILSGAILGIFLGYQ
                     KYRLHAASRNRLDEIRFGLASNMKFDIETGGFNLITKAAGAGSLGVVKDKKGNRQPAI
                     FFTTQNSTNDTMIVLSAAQTGTGSVVNLTGSKLTINGVDPEVWKQFPSGQLLLCTDTD
                     DSPVLIQLTGSPRKATQEECVVPATYVGGFSELPGGNTGGGTGDGNGGGPGRDGSTKV
                     PQRPDLPPITLPPGTGGGSTGGGTGGGIGTGGATGVGILDPERTVVASFSVVTNCSFF
                     SSIGTVPKLRGTLVPINQVVTYSISETGITRSETSGCQSEGQTAQLARFAPGYTVNLQ
                     FAYLVRAGGAVSASATLPSSAQNLVGVRVSGTLTDPTTRRTEAVQVDSILEALKPVQL
                     TIKTP"
     gene            complement(2098..3744)
                     /locus_tag="PKZ53_03045"
     CDS             complement(2098..3744)
                     /locus_tag="PKZ53_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ39439.1"
                     /translation="MASSFPPSPAESPSPSLWANRFETASRWALFLCALTAPHSIAAS
                     QTSFVLAVFCWLTQCAISRRWGLIPTPADWPLLLFFGLCLLSSAFSYEPRLSLKGMKS
                     VALLGAIYLVSSRVQNLRWVVILALTLLLSCQANTIFTLGQRAAGVGVRVTNFSKRSP
                     LLRAGLQPGDVILKADGQPIRSLETLSQLADAGTPDTSIKLQFRRGEQLLDSQIRRGA
                     ITKTSEKKLAKLRIKGADAKRLPQGVERLGAEGSLARDFRASGFYNHYATYAEVLQLL
                     ASLGIGLWLSLWKQPQLQREPNGLWIFRRLSPKQFQVLISVVLFLTLGALFCTNTRAS
                     LASLGLSIGVMLIALWKTGKIQSRTAAALLGLALIISCAGVALAYKQRSIALTDTSEG
                     SLYWRLVVWQEGVHLVEQHPVLGIGKDSDKIHGREWGLWGKGQLPPGHFHNSYLQIAV
                     WWGLPALLAYGWMVWLFWLALWKSVTDARFTSQPSLEHTCSYGVLLGVCGGFTGFAAS
                     SVVHFNLGDGEVTLILWFVLGLAVAARRLLASSPHTLTKI"
     gene            complement(3874..>4945)
                     /locus_tag="PKZ53_03050"
     CDS             complement(3874..>4945)
                     /locus_tag="PKZ53_03050"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ39440.1"
                     /translation="GEPDFTTPANIRDAAKRALDSGKTKYTPASGTAELKKAVLNYIE
                     RETSTRFEPSQIIISAGGKQTLFNAITTLINPGDEVIIPTPYWVTFPEIVTFCGGTSV
                     YIDTEDNGFQLTAEMVKKAITPRTRLIIVNSPSNPSGRIIETEEFRKIAELVAERGLW
                     MISDECYYQFTYPPHKPFSAASLPAELRERVLVSGSFSKTYAMTGWRIGYALANKDWV
                     TDMATVQSHSTSNPTTFAQWGAIEAAEGDQSSIAEMMAEYQKRRDWLVPALNELKGVS
                     CRMPEGAFYAFPSIKGVLEQSSLKADSEFTDLLLNEAYVVMTPGSAFGAPGYVRISYA
                     NSLENIQRAVDRVRQVIEKITA"
BASE COUNT         1235 a         1237 c         1342 g         1131 t
ORIGIN      
        1 gattggactt cgaacaccag tgggtcgttg atgttgagaa ccggggtatt ggctggaagc
       61 gagtttgtcg aaacttcggt caccaggcga actcgttttg agaggtccaa cgactggaaa
      121 ttgaccgtcc gacttgccgt gtcttccacc ccaaggcggg cattttgccg ggcttcttca
      181 accgcctggg caatgcgttc gagagcaatc aactcacgat tggcatttgg gccagcgttg
      241 ccggagaaat atccgatcaa tccagcaatc acgccaatga tcaaaatcac cgccaggact
      301 tcgattaagg aaaatccctg ttgcgtctga cgacggcgga agcggggttc agggttcagg
      361 gttcggagtt cagggttcaa tatcaatgtt cgaaacatag tcagctcctg gcagatgatt
      421 ctcacggggt tttgatcgtt aattgcacgg gtttcagggc ttcgaggatc gaatcaacct
      481 gaacagcttc cgttcgacgg gtcgttgggt cagtgagggt accgctgacc cgaactccaa
      541 ccaggttttg ggcacttgat ggaagtgtcg cggatgccga gaccgccccg ccggctcgaa
      601 ccaggtaagc aaattgaaga ttgacggtgt agcccggcgc aaaccgcgcc agttgagccg
      661 tttgtccttc ggattgacag ccagatgttt cagaacgggt gatgccggtt tccgaaatcg
      721 aataggtcac aacttgattg attggaacca gggtcccgcg cagtttggga acagttccga
      781 ttgaagaaaa aaatgagcaa ttggtgacga ctgaaaacga ggcaaccacg gttcgttctg
      841 gatctaaaat tccaactcct gtggccccgc cggtaccaat gccaccacct gtcccaccac
      901 cagtgctgcc gccgccagtt ccgggcggca gtgtgatggg cggcaagtct ggtcgttggg
      961 gtactttggt tgaaccatcc cggcctggac caccaccatt accatcgccg gttcctccac
     1021 cggtatttcc acccggaagt tcactaaacc caccgacgta ggtggctgga acgacacatt
     1081 cttcctgggt tgctttgcgt gggcttcctg tcagttgaat caaaacgggg gagtcatcgg
     1141 tatcagtaca gagcaataac tggccggagg gaaactgctt ccagacttcc gggtcaaccc
     1201 cgttgattgt gagttttgaa ccagtcaggt tgacaaccga gccggtgcct gtttgagcgg
     1261 ctgacaaaac gatcatggtg tcgttggttg aattctgagt cgtaaaaaaa atcgctggct
     1321 gacgattgcc ttttttatct ttgacaaccc cgagcgatcc ggcgccggca gctttagtaa
     1381 ttaaattaaa gcctccagtc tcaatatcaa acttcatatt ggaggcaagc ccaaaccgaa
     1441 tctcatccag tcggttgcga ctggcggcgt gcaggcggta tttttggtaa ccgagaaaaa
     1501 tacccaggat ggcaccgctg aggatggaaa agatcaacag cccgatcagg agctccataa
     1561 aggaaaatcc tgcttgccga tatgtgttct gtgtggagtg gaagtgtctg gccatagagt
     1621 gaagccttga ggaaattaaa ttattgaaat aaagcgatcc tgcctggttt aagtgcaaac
     1681 agtgttccag ggcaagaatc atggcatttt gtgctcgcag accaatggat cagtgcgccg
     1741 tctgtttccg ctcgatactg catataaact tttaccacct gcccaaatac aaaaatccag
     1801 acctgggccc aggtgtaaaa agagagtgat tctttgttaa attattgaat tgaaaggtaa
     1861 tgaatgtgtc atgagacgca ggtagctctc tggattctct atgaaagtcc tggtcgttga
     1921 gttgcacaat cgtgttttaa gtgagtgata ggactggtcc ctacagtcga gcgttctatt
     1981 ttgctcatat tttgaaaaaa agtgcaaact tctttttgaa aaaggagtta tcgcaccgca
     2041 caacccgtat ccacgctggc tttgctgaaa gtaattaaat taaaatttcg aattaaatta
     2101 aatttttgta agagtatggg gcgaggatgc cagcaatcgc cgggcagcga ctgccaaacc
     2161 aagcacaaac cagaggatga gggttacttc gccgtcgcct agattaaagt gcacgactga
     2221 gctggctgca aagccggtaa acccaccaca gacgccgagt aacaccccat agctgcaggt
     2281 atgctcaagc gaaggctgac tggtgaaacg tgcatccgtg acagatttcc aaagcgccag
     2341 ccaaaacagc cagaccatcc atccataggc aagcagtgcc ggaagccccc accacactgc
     2401 aatttgcagg taggagttgt gaaagtgacc cggcggaagc tgtccttttc cccacagccc
     2461 ccattcgcga ccatggattt tgtcactgtc tttgccaatg cccagaaccg ggtgttgttc
     2521 aaccaggtgc acgccttcct gccagaccac cagccgccag tacaaactcc cttcactggt
     2581 atcggtcagg gcaatgctgc gttgtttgta cgccagggcg acacccgcac aactgatgat
     2641 caacgccagt ccgagcaatg cggcagcagt tcggctttga attttcccgg ttttccaaag
     2701 tgcgatcagc attacaccaa ttgacaaccc gaggctggcc agcgatgcgc gggtattcgt
     2761 acaaaacaaa gcgccaagcg taaggaacag cacgacactg atgaggacct gaaactgctt
     2821 tggtgacaat cgtctgaaaa tccagagacc gtttggttcc ctctggagtt gaggctgttt
     2881 ccacaagctc aaccagagac caatgccaag cgaggccagg agttgcagca cttcggcata
     2941 tgtggcgtag tggttataaa acccgctggc tcgaaaatca cgggcgagcg aaccttccgc
     3001 tccaagtcgc tcaacaccct gagggagtct tttggcatcc gctcctttaa ttcggagctt
     3061 ggccagcttt ttttcgctgg ttttggtaat ggcgccccgg cgaatctggc tgtcaagcag
     3121 ttgttcgccc cgccggaact gaagtttgat cgaggtatcc ggcgttccgg catcggcaag
     3181 ctgggagagt gtttcaagac tgcggatggg ctgaccatca gctttcagga tgacgtctcc
     3241 gggttggagt ccggcacgca aaagcggact gcgtttggaa aagtttgtca cccgcacccc
     3301 aactccggcg gcccgttgcc caagagtaaa aattgtgttg gcctgacacg acagaagcag
     3361 cgtcaacgcc agaatgacca cccagcgcag attttggacg cggcttgaca ccagataaat
     3421 tgcaccgagc agggccacgc ttttcatgcc tttcaggctc aggcgcggtt catatgaaaa
     3481 agctgaagac agtaaacaca acccaaaaaa tagcaggagc ggccaatcag ccggtgttgg
     3541 aatcaatccc catcgccggc tgatggcgca ttgggtcagc cagcagaaaa ccgccaggac
     3601 aaaactggtc tgcgaagctg caattgaatg gggagcggtt aaggcacaca gaaacaacgc
     3661 ccaccgggag gcggtttcaa accggttggc ccaaagggaa ggagacggcg attcagccgg
     3721 tgatggaggg aaagatgaag ccatgaagca acgggacaga gaaaaatggc tttatattcg
     3781 gacgaaacaa aatctcacca cagaggacac agagaaacac agaggaattt gaagacacat
     3841 ctgtgctcct ctgtggcctc tgtggttcaa aaatcaggct gtaatcttct caatgacctg
     3901 gcggacgcga tccacagccc gctggatgtt ttcgagggaa ttggcgtagg aaattcggac
     3961 atatcccgga gcaccaaacg ccgagccggg tgtcatcacg acataggctt cgttgaggag
     4021 caggtcggta aattcagagt ctgctttgag tgaactttgc tccagcactc ctttaataga
     4081 agggaaggca taaaaggcgc cttcgggcat ccggcaggaa acgcctttga gttcgttcaa
     4141 agcgggaacc agccagtcac gccgtttctg atattcagcc atcatttcgg caattgacga
     4201 ctggtcgcct tctgccgctt caatggcgcc ccactgggca aaggtggttg ggtttgaggt
     4261 tgagtggctt tgaactgtcg ccatatccgt cacccaatct ttattggcca gcgcatagcc
     4321 gattcgccag ccggtcatgg cgtaggtttt tgaaaaagag ccggaaacca ggacccgctc
     4381 gcgaagttca gccggtaaac tagcggctga aaagggctta tgcggtgggt aggtgaattg
     4441 atagtaacac tcgtcggaaa tcatccagag cccacgttcg gccaccagtt cggcaatttt
     4501 gcggaattct tcggtttcga taattcggcc tgatggattg ctcggtgagt tgacgataat
     4561 caaccttgtc cggggagtaa tcgccttttt gaccatctcg gcggtgagct gaaacccgtt
     4621 gtcttcggta tcaatgtaaa ccgatgtgcc accgcaaaac gtcacaattt cagggaaggt
     4681 aacccaatag ggagttggga tgatgacttc gtcaccggga ttgatcaggg tggtaatggc
     4741 attaaagagc gtttgcttcc cacctgccga aataatgatt tgcgatggtt caaagcgggt
     4801 gctggtttcg cgctcaatat aattgaggac cgcctttttc agttcagcag taccgctggc
     4861 tggcgtgtat ttggttttgc cactatcaag ggcacgtttg gccgcgtcgc gaatgttggc
     4921 tggcgtggtg aaatcgggtt caccc
//
LOCUS       DAOQDO010000123         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11184_length_4927_cov_3.436782, whole genome shotgun sequence.
ACCESSION   DAOQDO010000123 DAOQDO010000000
VERSION     DAOQDO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11184_length_4927_cov_3.436782"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..2820)
                     /locus_tag="PKZ53_03055"
     CDS             complement(55..2820)
                     /locus_tag="PKZ53_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNJ39441.1"
                     /translation="MSPWKFASLPRCATWMFSVVGLLIFLVGNLALPRSQASAPLPAQ
                     EVNLPANFSQALVVSGISNPTTMDFAPDGRLFVCQQNGNLRVIKDDILLTTPFLTVTV
                     SSAGERGLIGLTFDPNFAVNRFIYVYYTATSPNIHNRISRFTANGDVVVPGSEQVLVD
                     LPATTNTNHNGGALHFGPDGKLYIAVGEDGVPSRAQDLANPFGKILRINADGTIPTDN
                     PFFNSTTGISRAIWAYGLRNPFTFGFQPGSGRMFINDVGQNTWEEINDGIVGSNYGWP
                     TTEGATTNPLFRSPIYAYPHGAGTFSGCSIIGSTFYNPTTVRFPQQYVGSYFFADYCS
                     GWINRLDPQNGNQVTNFHTGFSSAVDVRTSDDGRLYVLTRGNGGAVYRIDYSGPTQAP
                     QITQQPASQTVAVSQTATFTVTASGQQLSYQWQKNSVNISGAINASYTTPATTLQDNN
                     AQFRCVVSNTAGTATSDPATLTVVNSQPPAPTIVTPTAGTLYTAGDTIFYSGTATDPD
                     EGTLAGSAFTWWVDFHHDTHTHPHVLPTTGSTSGSFVIPTGGETASNVWYRIYLRVTD
                     TTGLSTTVFREIFPRTATITLASNPSGLALTLDGQPVTAPFNFVGVAGITRTLGVVSP
                     QTVGGTQYQFVSWSNGGAATQNISTPLINTTYTASFQAVPQPPTITTHPANQTVSVGQ
                     SATFTVVATGTPSPGYQWQRNNVNITGATSASYTLASTSVSDNGAQFRCVVSNSQGSA
                     TSNQATLTVVSGPQTFAISSDAYVRNGSFANANYGRVNALYANNSNSAGAARVSYLQV
                     TLQNVSPTFTSAKLRVYGRLLNSLQRNIGVSVYPVTSTTWQETTLTWNNRPAAGATAL
                     GSATIVNNQFRWYELDVTSYVRSELQAGRSTISLMLAIPVLNTKTYPTFNSREALTNR
                     PEIMVTF"
     gene            complement(3204..>4927)
                     /locus_tag="PKZ53_03060"
     CDS             complement(3204..>4927)
                     /locus_tag="PKZ53_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNJ39442.1"
                     /translation="PDGRIFICLQNGTMRLVKNGSLLPDPVIVLPVNSIGENGLIGIA
                     LDPQFTTNQFIYVHYTATSPTLHNRVSRFTLDGDTILSGSEVILLELPTITHNAHNGG
                     ELNFGHDGKLYIGVGDNTSSVFAQGFSTPFGKILRINPDGSIPPDNPFLEMTTGVNQA
                     IWAYGLRNPFSFAFQPGKGPMYINDVGQDLWEEINLGKAGANYGWPVTEGPTTEPSFT
                     TPVVAYPHGEGLTAGCSIVGAAFYNPTTVRFPSQYVGRFFFGDLCNGWIASLTPQGQF
                     VHFQSGFIYPVDVAVSSDGFLYVLAEGGTSGALYQIDNANYGMAEIIQPPVNQYVGVG
                     QTATFTVVASGIDLTYQWQRNGVNIAGATSSSYTTPPARTQDSGAQFRCRVSNQLNAV
                     TSTSAILTVLTTPKTVFSIADTYVRNGSNANRNFGTAPTLIVEQSASTSSIQYTYVRF
                     DSRAIQTPIASAKLRIFGNLSSSLQRNVPLAIYPVSDTTWRETSITWNTRPPAGTLPI
                     TNVTMRDTTSRWYEIDVSPYVLSEIQAGRRVFSFMIANTAPAKVFSQFNSRQNASFGP
                     QLVVITQ"
BASE COUNT         1220 a         1147 c         1367 g         1193 t
ORIGIN      
        1 ggaacccgga acccggaacc cggaacccgg aacccggaac ccggaaccct gattttaaaa
       61 agtcaccata atttccggac ggttggtcaa ggcttcccgt gaattaaagg tgggataggt
      121 cttagtgtta agcactggaa tggcaagcat tagactgata gttgaccgtc cggcctggag
      181 ttctgaccga acataactgg tcacgtccaa ttcataccac cggaactggt tatttacgat
      241 tgtggccgaa ccaagtgcgg tggcacccgc cgccgggcga ttgttccagg tgagggttgt
      301 ttcttgccag gtggtgctgg taaccgggta gactgagact ccgatattgc gctgaagcga
      361 gttgagtagc ctgccgtaaa ccctcagttt ggctgaggta aaggttggcg aaacgttttg
      421 gagcgtgacc tgcaggtagg aaacccgtgc ggcaccagcc gaattgctgt tattggcata
      481 gagggcattc acgcgaccat aattggcatt ggcaaatgat ccatttcgga catacgcatc
      541 cgacgagatg gcaaacgtct gtgggccact caccacggtt aatgtggctt gattactggt
      601 ggctgaaccc tgcgaattgg aaaccacaca gcggaactga gccccattat cactcacgga
      661 tgttgaagcc agggtgtaac tggctgaagt tgctccagta atgttgacat tgttgcgttg
      721 ccattgatag cccggtgacg gtgttcctgt ggccaccaca gtgaaggtgg ccgattgtcc
      781 aactgaaacg gtctggtttg ccgggtgggt tgtaatggtt ggtggctgtg gcaccgcctg
      841 gaagctggca gtataggttg tgttgatcaa tggcgtggag atgttttgag ttgccgcccc
      901 gccatttgac caggaaacaa actgatattg tgttcctcct acggtttgcg gtgagaccac
      961 cccgagtgtg cgtgtgattc cggcaacacc aacaaaattg aatggcgctg tgacaggttg
     1021 tccatccagc gtcaacgcca atccagacgg atttgaagcc agcgtgatcg tggcagttcg
     1081 tggaaaaatt tcacgaaaga cagtggttga cagaccagtt gtatcagtga ctctcaaata
     1141 gatccgatac cagacattgc tggccgtttc accaccggtt ggaataacaa atgaacctga
     1201 agttgacccg gtggttggca gtacgtgggg atgggtgtgg gtatcgtgat gaaaatcaac
     1261 ccaccaggta aaggcactcc cggccagcgt tccttcatcc ggatcggtgg cggtgccgga
     1321 atagaaaatg gtgtcacctg cggtatagag cgttccagcg gttggggtca caatggttgg
     1381 tgctggtggc tggctgttga cgaccgtcaa tgtggccggg tcgctggttg ccgtgccagc
     1441 agtattggaa accacacagc ggaattgggc attgttgtcc tggagcgtgg tggccggagt
     1501 ggtatagctg gcattgatgg caccagaaat attgacactg tttttctgcc actgatagct
     1561 caactgctga ccactggccg tcactgtaaa cgtggcagtc tggctcaccg ctacggtttg
     1621 actggctggc tgctgggtaa tttgtggcgc ctgggttggt ccactatagt caatgcgata
     1681 gaccgcacca ccgttcccac gtgtgagcac atagaggcga ccgtcatcag aggttctcac
     1741 atcaacggct gagctaaatc cagtatgaaa atttgtgacc tggtttccat tttgaggatc
     1801 caggcgatta atccagccgc tgcagtaatc ggcaaagaaa tagctcccga catactgttg
     1861 tggaaaccga accgtggttg ggttgtaaaa ggtagatccg atgattgagc atcctgaaaa
     1921 tgtacctgct ccatgggggt aggcatagat tgggcttcgg aagagaggat tggtggtggc
     1981 gccttccgtg gtgggccacc cataattgga cccgacgatg ccgtcgttga tttcttccca
     2041 ggtattttgc ccgacgtcat tgataaacat ccggcctgat cctggctgaa acccaaaggt
     2101 aaatggattg cgcaggccgt aggcccaaat ggcccggcta atacccgtgg ttgagttaaa
     2161 aaatgggtta tcggttggaa tcgtcccgtc agcattgatc cgcagaattt ttccaaaggg
     2221 attcgccaga tcctgggccc ggcttggaac gccgtcctct ccgaccgcaa tatataactt
     2281 gccatccggc ccgaaatgga gcgccccacc gttgtggtta gtgttggtgg tggctggcaa
     2341 atcaaccagc acctgttcac tgcctggaac aactacgtcg ccattggcgg taaagcgact
     2401 gatccgattg tgaatattgg gcgatgtggc ggtgtagtac acataaataa atcggttgac
     2461 ggcaaaattg gggtcaaacg taaggccaat taacccgcgt tcgcccgctg aactcaccgt
     2521 caccgtgagg aagggtgtgg taagtaaaat atcatcttta atgacccgga ggttgccgtt
     2581 ttgctggcaa acaaacaggc ggccatctgg agcaaaatcc atggtggttg ggtttgaaat
     2641 accactgacc accagcgcct gtgaaaaatt ggcaggaaga tttacttctt gagccggaag
     2701 cggtgccgat gcctgtgaac ggggcagggc caggttccca accagaaaaa tcaacaaccc
     2761 aactacagag aacatccagg ttgcacaacg agggagcgac gcaaacttcc agggagacat
     2821 atcgggttca ccctttcatg gcgataagcg agagatttca cacaacgtga gtatgacttt
     2881 agcaggactt cgggtggacc tcgaattcct ggtgcgggag ggggaagatg attgggggaa
     2941 tttgagtgcc tggacaggca cgacacccgc gattatacag atgaagttat ccggatggaa
     3001 aaaaacaatg ccggaaaatt attatatttt tccagctttt tctggagaaa catctcgact
     3061 gacaacgggt gtcaggctga acaaggggtg gtggataaca gcgggttgaa ggattctgaa
     3121 gtggcttcac ttcgcagcgt aatatgcctg gtctacttcc ttcaacccga ggtatttttc
     3181 cctgcctgat ggaaaggccg aggttattgt gtaatgacaa ccagttgagg cccaaacgag
     3241 gcattttggc gtgaattgaa ctgtgaaaac accttggctg gagctgtatt ggcaatcata
     3301 aagctgaaca ctcgccgacc agcctggatt tctgacagta cataagggga aacatcaatt
     3361 tcataccacc ggctggttgt gtcgcgcatg gtgacatttg tgatcggcag ggttccggct
     3421 ggggggcggg tattccaggt tatactggtt tccctccagg ttgtatcaga aaccggataa
     3481 atggcaagcg gaacattgcg ctgaagtgaa ctggacagat tcccaaagat acgcaatttg
     3541 gctgaggcaa ttggcgtttg aatcgctctg gaatcaaacc gcacatatgt atattggata
     3601 ctgctggttg acgctgactg ctcgacgatg agcgttgggg ccgtcccaaa attccggttg
     3661 gcattacttc catttcgaac gtaggtatct gcaatggaaa aaactgtttt aggcgtggtg
     3721 agcacagtta atatggctga agtactggtg acggcattga gttgatttga aacacgacaa
     3781 cggaattggg ccccactgtc ttgggtccgg gccggtggcg ttgtatacga cgaactggtt
     3841 gcgcccgcaa tatttactcc attgcgttgc cactgatacg tgaggtcaat cccgctggcc
     3901 acaaccgtaa atgttgctgt ttgaccaact ccgacatatt ggttgaccgg gggctgaatg
     3961 atctcagcca tcccatagtt tgcattatca atttgataga gcgcccctga ggttcctcct
     4021 tccgccagta cgtacaaaaa gccatccgaa ctgactgcca catctaccgg gtagataaac
     4081 cctgattgga aatggacaaa ctggccctgt ggtgtcagtg atgcaatcca cccattgcat
     4141 aaatcaccaa agaagaagcg tccgacatat tggcttggaa atcgcacggt tgttgggttg
     4201 taaaaggctg ctcccacaat cgaacagcct gcggtcagtc cttcgccatg cggataggcc
     4261 acaacaggtg tggtaaacga cggctccgtg gttggtcctt cagtgaccgg ccatccataa
     4321 ttggcgcctg cttttcctaa gttgatctct tcccacaagt cctgaccgac atcattgata
     4381 tacattgggc ctttgccggg ttgaaaggca aatgaaaaag ggttgcgtaa cccataagcc
     4441 caaatggcct gattgacccc agtggtcatt tcaagaaatg gattatcagg tggaatgctc
     4501 ccatcagggt taatccgcag aattttgcca aacggagtcg aaaacccctg ggcaaatact
     4561 gatgaagtat tatcgccaac accgatatag agttttccat cgtgtccaaa attgagttct
     4621 cctccattat gggcgttatg cgtgatcgtt ggtagctcca gtaaaataac ttcggatccg
     4681 gaaaggatcg tgtctccatc cagggtaaac cgactcacac gattgtgaag ggttggggat
     4741 gttgccgtgt aatgcacata aatgaattga ttggttgtaa attgcgggtc cagagcaatt
     4801 ccaatcaatc cattttcacc aatggaattc actggtaaga caataacagg gtctggaagc
     4861 aaactcccat ttttgaccag gcgcatagtt ccattttgaa gacagataaa gattcgacca
     4921 tccgggg
//
LOCUS       DAOQDO010000124         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11194_length_4925_cov_3.244764, whole genome shotgun sequence.
ACCESSION   DAOQDO010000124 DAOQDO010000000
VERSION     DAOQDO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11194_length_4925_cov_3.244764"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..3885)
                     /locus_tag="PKZ53_03065"
     CDS             complement(10..3885)
                     /locus_tag="PKZ53_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39443.1"
                     /translation="MSVTRDLMEYGRPNIRVFTDKEGLPQNAITSLVFDQEGYLWAGT
                     KDGVARYDGRSWTVVNLPKELKTNWIHCLMAASDGSLWVGTHEGLAQLDRGRWRCFTK
                     TDGLPDNEIRCLKEISNGQGGWNLWVGTWRGLARWTGQQWQTEPPFPSNSSPEISSLL
                     ERKSPDGHQEIWVGCPKSGIARWREGVWTVYDQYHGLPSEGVTCLLETTALDGQRRLV
                     AGTFTSDLMVFNESHWTSLAGTPLSLGGGGAHCLLETQSPTGEPVLWAGNAGGLKCLV
                     EKRWLSWRERQGMSGLLIWCLAQAPGPNGTVSLWAGTAGEGIIHWQLGTWMRFDKTSG
                     LPENSTYSLLVTQDEAGADVVWLGSNTKGFSRLNPDGTWTYFNEANWVLDAGIRSFCE
                     TRDPDGRSELWVGTDHLKLCRFKNGQWTVFPILGENQSLLVWCIVPTRASDGTPALWL
                     GTEEGLFYLPNEATVPTKPAWKLPDKNVLAILETASPQGTILWLGTRQGLVRIAQGQI
                     TDIFDARSGLPGNQVLSLREYRTLTGERSLWVGTNLGVARLALDSPTPKWSYLSATST
                     PALPNNTIYRIEEDARHRLYLCTNKGVARLTPRTPTPDDASEFEIYTYTTDDGLPSNE
                     CNTGASTVDHRGRIWVGTIAGVAVLDPAREVTNRIQRPLLIQQAVLPDQGRVLRDGDV
                     LAYDENHIQFEFALLSFFREGEIRYRTQIEGLDPHPSAWTKDFSRDYISLPPGRYRFQ
                     VWGRDVFGNVSGPVGMSFTIRRAPWLTWWAVVGYTLTLGFGGYGIFRYRLRTLRHRNE
                     LLQAKIAERTSQLAQTVEELRDSEHRALEANRAKSVFLANMSHELRTPLNAILGFVQL
                     MDRDASLPVLHRESLGIISRSGQHLLGLINDVLSIAKIEAGKITLVETTFDLSQLLRS
                     VRDMIRIRAEAKELIVNFVLDPNLPTVVLGDEGKLRQVLVNLLGNAVKFTSQGSVTLT
                     ATWKSRGDQSGQLQFEIRDTGCGISESDCARLFEAFVQTESGRKMQEGTGLGLVISRD
                     FVRLMGGDIRVSSILGQGTTFHFDVVLQVATQVEAKDVHREVIGLAPNQPGWRILVVD
                     DTEENRLLLTRLLQSVGFQVRESSDGAEAVALAGSWKPDLIWMDMRMPVMDGLEATRH
                     IRAQNSGENHSGKPVIIALTASAFDHEQERFLRAGCDDYVPKPFEVPVIFEKMARYLG
                     VKYVYEESELPVSGSTGSVLTSDRLRNFSPDAIQSLTTALMAGDTELAIALIENVRQQ
                     DASLADELAGLVRSYRFDEILDWLEGREG"
     gene            complement(4176..4592)
                     /locus_tag="PKZ53_03070"
     CDS             complement(4176..4592)
                     /locus_tag="PKZ53_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39444.1"
                     /translation="MGHQISFSICHEYDAGDREITVPLMLTSGSNQIEVNGKLDTGAS
                     SCVFPREIGEFLGIDIESGEVQRFSSVHSSSIAYGHWLQISVFGIDFEATVYFVKEPG
                     YPRLLLGRKGFLEKFRIGLIEYDGLLYLSEYNEDQE"
     gene            complement(4594..>4925)
                     /locus_tag="PKZ53_03075"
     CDS             complement(4594..>4925)
                     /locus_tag="PKZ53_03075"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HNJ39445.1"
                     /translation="FDEYAVGKFIFVDQMTGDILPKDTNVPEIHHSREEKRFIASFRW
                     ACNRTWLKRNRDRYIGEWVALSEGVLVSHGINLGEVLKTARAKGYQFPQVTYIDPAGE
                     KMGEELV"
BASE COUNT         1199 a         1409 c         1193 g         1124 t
ORIGIN      
        1 ccctatcccc tatccttccc gcccttccag ccaatccaga atttcatcaa agcgatagct
       61 ccgaaccaga cctgccagtt catcggccag tgacgcgtcc tgctggcgca cgttttcaat
      121 cagagctatc gctaattcag tatctccggc catcaaggct gtggttaatg attgaattgc
      181 atccggggag aaatttcgca accggtcaga ggtcaagact gaaccagtcg aacctgacac
      241 tggcagctca ctttcttcat agacatattt cacacccaaa tacctggcca tcttttcaaa
      301 aataaccggg acttcaaacg gttttggaac atagtcatca caccctgcac gtaaaaaccg
      361 ttcctgttca tggtcaaagg ccgaggcagt gagggcaata atcaccggtt tcccagagtg
      421 attttcgcct gaattttgag cgcggatgtg ccgggttgct tccaatccgt ccatcaccgg
      481 cattctcata tccatccaaa tcaaatcagg cttccaggat ccagccagcg ccacggcttc
      541 agcgccgtct gaagactccc gaacttgaaa gccaactgat tggagcagcc gggtgaggag
      601 taaccggttc tcttcggtat cgtccacgac caggattcgc caccctggtt gattgggtgc
      661 cagtccgatt acttcccggt gcacgtcttt cgcttcgacc tgcgttgcca cctgaagcac
      721 cacgtcaaag tggaaggtgg tcccctggcc gaggatactt gaaacccgaa tgtccccgcc
      781 catcaagcgc acaaaatcac ggctgatgac aagccctaac cccgttccct cctgcatctt
      841 gcgaccactt tcggtttgaa cgaacgcttc aaacagccgg gcacaatctg actccgaaat
      901 gccacagcca gtatcacgaa tttcaaattg gagttgccct gactggtcac cacgactttt
      961 ccaggtggca gtcagtgtga ccgagccctg actggtaaat ttcacggcat tgccaagcag
     1021 gttgactaat acctgtcgga gctttccttc atctcctaag acaactgtcg gaaggttggg
     1081 gtcaagcaca aagtttacga tcagttcttt ggcttcagcg cgaattcgga tcatgtcgcg
     1141 aactgatcgc aaaagttggg acaggtcaaa tgttgtttca acgagggtaa ttttcccggc
     1201 ttcaatcttg gcaatcgaaa gcacatcgtt gatgagaccc agcaagtgtt gaccgcttcg
     1261 ggagataatc ccgagcgatt cccgatgcaa gacgggcaaa ctcgcgtcac gatccatcaa
     1321 ctgaacaaat ccgagaatcg cgttgagtgg ggtgcgcaat tcgtggctca tattggccag
     1381 aaaaacactt ttggcacgat tggcttcaag ggcccggtgc tcagaatcgc gcaactcctc
     1441 gacggtttgg gcaagctggc tggttcgttc ggcaatcttt gcttgcagga gttcattgcg
     1501 atgccggagg gttcgcaacc gatatcgaaa gatcccatac cccccgaaac ccagggtcag
     1561 ggtgtaccca acaactgccc accacgtcaa ccacggcgcc cgtcgaatgg taaacgacat
     1621 ccctactgga ccagagacat tgccaaatac atcgcgcccc cagacttgaa accggtacct
     1681 gcccggcggg aggctgatat agtcacggct aaaatccttt gtccaggctg aaggatgtgg
     1741 atcaagtcct tcaatctggg tgcggtatcg gatttctcct tcccgaaaaa agctcaggag
     1801 cgcgaactca aactgaatat ggttctcgtc ataggccaac acgtcaccat ctctgagcac
     1861 ccgtccctga tcaggaagaa cggcctgctg aatcaacaac ggcctttgga tccgattggt
     1921 cacttctctg gccgggtcca gaactgccac tccagcaatc gtcccaaccc aaatcctgcc
     1981 gcgatgatca acggttgacg caccggtatt acattcattg ctgggaagac cgtcatcagt
     2041 ggtgtaggta tagatctcaa actcagacgc gtcatctggg gtcggtgtgc gtggggtcaa
     2101 tcgtgcaacc cctttgttgg tacataaata cagccgatgt cgtgcgtctt cctcaatccg
     2161 gtaaatggta ttattgggga gtgccggggt ggatgtggcg gaaagatatg accatttcgg
     2221 ggttggtgaa tcaagcgcca accgggccac gcccagattg gtgccaaccc agagactgcg
     2281 ttcgccggtc agagtccggt actcgcgcag tgaaagcacc tggttccccg gcaatccgga
     2341 ccgggcatca aaaatatcgg taatctgccc ctgggcaatc cggacaagtc cttgtctggt
     2401 ccctaaccag agaatcgttc cttgtgggga agctgtctcc agaatggcca ggacgttctt
     2461 gtcaggtagc ttccaggccg gtttggtcgg aacagtcgcc tcgtttggca agtaaaaaag
     2521 accttcctcg gttcccaacc acaaagccgg tgtgccatct gatgctctgg ttgggacgat
     2581 gcaccagacc aacaacgact gattctcccc gagtatggga aacaccgtcc attgcccgtt
     2641 cttgaaccgg cagagcttga gatgatcggt tccgacccac agctcggacc ggccatctgg
     2701 gtcccgagtt tcgcaaaacg agcgaatccc ggcatccagc acccaattgg cttcattgaa
     2761 ataggtccag gtgccatcgg ggttcaagcg ggaaaagcct tttgtgttag accccagcca
     2821 aacgacatcc gcccctgctt catcctgagt gacgagcaaa ctgtaggtcg agttttccgg
     2881 cagcccactg gttttatcga accgcatcca ggtgccgagt tgccaatgaa taataccttc
     2941 gccggctgtc cctgcccaca gcgaaaccgt tccattcggt cctggcgcct gggccagaca
     3001 ccaaatgagc aggcccgaca ttccctgccg ctccctccag gaaagccaac gtttctccac
     3061 gagacacttc aatcccccgg cgttccccgc ccagaggacc ggttctcccg tcggggattg
     3121 ggtttcgagg aggcaatggg cgccgccgcc gccgaggctc agcggcgttc cagccagcga
     3181 cgtccagtgg ctttcgttaa agaccatcag gtcactggta aaggtcccgg caaccagtcg
     3241 gcgctgaccg tcaagggcgg tggtttcaag caaacaggtc actccttcgg aaggaagccc
     3301 atggtactgg tcataaacgg tccagacacc ctcccgccac cgggcgatac cactctttgg
     3361 acaaccaacc caaatttcct ggtggccatc tggcgacttc cgttccagca ggctggaaat
     3421 ttcgggactg gaattggagg gaaaaggggg ttcggtttgc cattgctggc cagtccaccg
     3481 ggccagccca cgccaggtcc ccacccacag attccatcca ccttgcccgt tgctgatttc
     3541 ttttagacag cgaatctcgt tgtccggcaa accgtcagtt ttggtaaagc accgccaccg
     3601 tccccgatca agctgtgcca gcccttcatg ggtgcccacc cagagactgc cgtccgaagc
     3661 cgccatcagg caatgaatcc agttggtttt taattctttc gggagattga ctactgtcca
     3721 cgaccgaccg tcatagcggg ccaccccgtc cttggtacca gcccacaaat atccttcctg
     3781 gtcaaaaaca agggaggtaa tggcattttg cgggaggcct tctttgtcgg tgaagacacg
     3841 aatattgggc cgcccatact ccatcaggtc acgggtcaca gacacacctg aagccgcctg
     3901 agtcagcggt gtcagtgact gggaagcagc agaaattggg agggaaaagc acaaccaaag
     3961 caggagtgtt gaaaaccacc tgacggataa catacagcac acaatgtaac tgcagcaccg
     4021 tcagcgagta aagggtttcg aacaaccttc tgttttcaac cgacagcgtg gttgaggatg
     4081 agcagggttg agctcagcag gagggtttac aatgtcgttg aacccaaaaa ccaaaccggc
     4141 tggcagattg aagattcgcc agccggttta aactttcatt cctggtcttc attgtactca
     4201 gacaggtaaa gcaacccgtc atattcaatg agaccgatcc ggaatttctc taaaaaccct
     4261 tttcgtccaa gtaataatcg gggatatcct ggctccttca caaaataaac agtggcttca
     4321 aaatcaattc caaacactga aatctgaagc cagtgtccat aagcaatcga agatgaatga
     4381 acactgctaa atcgttgaac ttctccactt tcaatatcaa ttcctaagaa ttccccgatt
     4441 tctcttggga aaacacaact ggaagcccct gtgtctaact ttccgttcac ttcaatctga
     4501 tttgatccag atgtaagcat gagaggaact gtgatttccc ggtctccagc gtcatattcg
     4561 tggcaaatcg agaaagaaat ctggtgtccc atctcaaacc aattcctctc ccattttttc
     4621 tccggctggg tcaatatagg tcacctgggg aaattgatac ccttttgccc ttgccgtttt
     4681 taacacttca ccgaggttta tgccgtggct gactaacacc ccttctgaaa gtgccaccca
     4741 ttctccgatg taccggtccc ggtttctttt taaccaggtt cgattacagg cccagcgaaa
     4801 agaagcaata aaccgctttt cctcccgaga atgatggatt tcaggaacat tagtatcttt
     4861 tggcaagata tcacccgtca tttgatcaac aaagataaat ttccccactg cgtattcatc
     4921 aaaat
//
LOCUS       DAOQDO010000125         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11207_length_4920_cov_2.920247, whole genome shotgun sequence.
ACCESSION   DAOQDO010000125 DAOQDO010000000
VERSION     DAOQDO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11207_length_4920_cov_2.920247"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="PKZ53_03080"
     CDS             <1..1506
                     /locus_tag="PKZ53_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ39446.1"
                     /translation="GMPAAISLSGTLPLEAKTTHKPAEASPSVEMRINDRQTRFLNLE
                     LLREQLKRPEGTKVDQTVQRLSKLYEITHTLLPAKSVQELVDKWLEAIFTCLPCERGA
                     ILLHNSETGEFEQVVARDRETGKCGQIHTSFTIIEQTFRENVAVLCRDATSDERFASQ
                     KSIVIQNLRSVLCAPISSNLRVWGVCYLYNTKAIAQFNGEDLEFLMATAREAGLVFEN
                     LQLVGHLEALVAERTAEIVRQAEEIQHKNNHIMDSIRYAERIQQAILPHDHRVSQLFP
                     EHFILFRPKDIVSGDFYWVNEIDGVVIAAVADCTGHGVPGALMSMIGNTLLNQIVNEK
                     RILDPALILEHLHNGIREALRQEHAESESQDGMDLCLCRIEPGRVVYAGSRRPLYVVT
                     RQPDQPELIEIKGDRKSIGGMQKEDKRVFTNHEFQVTEPVMLYLSSDGFADQPSEQGQ
                     KFSTRRFREFLTHLARHSAERQYELIVAELEAHQGNEVQRDDLTVVGICVG"
     gene            complement(1551..3107)
                     /locus_tag="PKZ53_03085"
     CDS             complement(1551..3107)
                     /locus_tag="PKZ53_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39447.1"
                     /translation="MLYSVRSRLTIWYLAVLAVVLGVFAIVVYQIFVHQLMQRVDATL
                     HETAVSFSDAFYHELAEHQQMGVKSGADAIIQATREFRFRDRQLLILDPSHTLIATSL
                     VQLSEPATLAQADIVQPYLQHLAPQFLAQTEQEKSVFDTFLLNSADKIRAHGVLTTVG
                     GDQYIVIVVFNLKDYDAMLRRIQSSIWIAVLAALIISSFGGYFLALKSLSPVLVMSQQ
                     AEQIGATNLYERLDILNERDELGQLARVFNHMLARLQRAFDQQRRFMADASHELRTPV
                     AIVRGEAEVALSQHERTETDYRESLGIIQNEGRRMTRIVEDLFTLARADAGQHPLIIT
                     DCYLDDLVEECVQAVRTLAMQRKISVVNLTRIEIPYQGDEGLLQRMILNLLNNAIKYT
                     QIGGKVVISVHESEHHWMIDITDNGPGIPDDAQELIFERFYRVNKAASRADASSGAGL
                     GLPIARWIAEAHQGALRLKSSTSSGSTFTVFLPKKTVLATPGARRQTGNLVWVTPHSN
                     PGLSGDRVTK"
     gene            complement(3127..3813)
                     /locus_tag="PKZ53_03090"
     CDS             complement(3127..3813)
                     /locus_tag="PKZ53_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ39448.1"
                     /translation="MRILLVEDDPQVARFVAKGLREQAYAVDVANDGETALYQAAINQ
                     YDLIILDVLIPVKNGFEVCQDLRTQGVQLPILMLTACDSVENRITGLDYGADDYLTKP
                     FDFGELLARIRALLRRRGKTQPFQLMIADLVIDTRSRTAKRAGQTLDLTTKEFALLEF
                     LALNHGRVIGRAEIAEHVWDENFDPFSNLIEVYINRLRRKVDDGHSLHLIHTRRGAGY
                     ILECDNPTPL"
     gene            4424..4795
                     /locus_tag="PKZ53_03095"
     CDS             4424..4795
                     /locus_tag="PKZ53_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39449.1"
                     /translation="MGLVQRVRSVSQQMVMVYDEVKIHREIDQLEREVSQNPTSVFHL
                     QQLCNLYQTAGYIEKATSVLCQLASLHRDRREPELTLAFLRKAERMCLPEQRISLLHQ
                     IVVDLPSRIRISSECARCWRF"
BASE COUNT         1271 a         1283 c         1231 g         1135 t
ORIGIN      
        1 ggcatgccgg cggcgatttc actcagtgga acgctcccgc ttgaagccaa aactacacat
       61 aaacccgccg aagccagccc ctcggtggag atgcggatta acgaccggca aacccgattc
      121 ctcaatctcg aattactgcg cgaacagctc aagcggcccg aagggacaaa agttgatcaa
      181 actgtccagc ggctcagcaa attgtatgaa atcacacaca cgctgttgcc agccaaaagc
      241 gtccaggaac tggtagataa atggcttgaa gcgattttta cctgtcttcc ctgtgaacgg
      301 ggcgcgattt tgctccacaa ttcagagacg ggagagtttg agcaggtcgt ggcccgcgac
      361 cgtgaaaccg gcaagtgcgg ccagattcat accagtttca ccatcattga acagacgttt
      421 cgtgaaaacg ttgcggtctt gtgccgggac gcaaccagtg atgaacggtt tgccagccag
      481 aaaagtattg tcatccaaaa tctgcgctca gttttatgtg ctccgatcag ttcaaatctg
      541 cgtgtgtggg gcgtttgtta cctgtataac accaaggcga ttgcccagtt taacggcgaa
      601 gacctggaat ttctgatggc cactgcccgc gaagccggcc tggtgtttga gaatctgcaa
      661 ctggtcggcc atcttgaagc cctggtcgcc gaacgaaccg ccgaaattgt ccgccaggct
      721 gaagaaatcc agcacaaaaa taaccacatc atggatagca tccgctatgc ggaacgcatt
      781 cagcaggcaa ttcttccgca tgatcaccgc gtcagccagc ttttccccga acacttcatc
      841 ctgtttcggc ccaaagatat tgtgtcaggt gatttttact gggtgaatga aattgatggg
      901 gtggtgattg cggcagtggc tgactgcacc gggcatggtg tccccggagc cttaatgtca
      961 atgatcggca acacgctgct caatcagatt gtgaatgaaa agcggatttt ggatccggcg
     1021 ctgatcctcg aacacctgca caatggcatt cgcgaagcac tccgccagga acacgccgaa
     1081 agtgaatcgc aggatggcat ggacctctgt ttatgtcgga ttgagccagg acgggtggtg
     1141 tatgccggga gccgccgacc gctctatgtg gtaactcgtc aaccagatca accagaactg
     1201 attgaaatta aaggtgatcg gaaatcaatc ggcggtatgc aaaaggaaga taaacgggtt
     1261 tttaccaacc atgagtttca agtcactgag ccagtgatgc tctatttatc ttcagatggc
     1321 tttgccgatc agcccagcga acaagggcaa aagttcagca cccggcgatt tcgggaattt
     1381 ttaacccacc tggcccggca ttctgccgag cgtcaatatg aattgatcgt cgccgaactc
     1441 gaagcccatc aaggaaatga ggtccaacgt gatgacctga ccgtggtcgg gatttgtgtt
     1501 ggatgaacgg cagggtcagg ggtcaggggt ttcaaatcaa tctcacgtcg tcactttgtc
     1561 acccggtcac ctgacagacc tggatttgaa tggggcgtta cccacaccaa atttccagtt
     1621 tgacgtcggg cgccaggagt ggccagaacc gttttcttgg gaaggaatac tgtgaaggta
     1681 cttccgctgg acgtcgaaga cttcagtcgg agtgctccct gatgcgcttc agcaatccac
     1741 cgggcgatag gaagccccag cccggcaccg cttgacgcat cagcgcgtga ggcagcttta
     1801 ttgacccgat agaaccgttc aaaaatcagt tcctgggcgt catcaggaat gccgggacca
     1861 ttgtcggtaa tatcaatcat ccagtgatgc tcactttcat gcactgaaat tacaaccttg
     1921 cccccaatct gggtgtattt gatggcattg ttgagtaaat tgagaatcat ccgctgtaac
     1981 aacccctcgt caccctggta tggaatttcg atccgagtca aattcacgac tgaaattttg
     2041 cgctgcatgg ccagcgttcg caccgcctga acacattctt cgaccagatc atcaaggtaa
     2101 caatcagtaa taatcaacgg atgctgcccg gcatctgccc gcgccagtgt aaacaaatct
     2161 tccacaatcc gggtcattcg ccggccttca ttctggataa tccccagtga ttcacgatag
     2221 tcagtctcgg ttcgctcgtg ttgagaaagg gcgacttctg cctcgccacg aacaatcgcg
     2281 accggagttc tcaattcatg cgaagcgtca gccataaatc gccgttgctg atcaaacgcc
     2341 cgttgcaacc gagccagcat atggttaaac actcgtgcaa gctgccctaa ctcatcgcgt
     2401 tcattgagga tatcgagtcg ttcgtacagg ttggtcgccc caatctgctc agcctgctgt
     2461 gacatcacca gaactggcga tagacttttc aaggccagga aatagccgcc aaaactggaa
     2521 atgatcagtg ccgccagtac ggcaatccag atggaggact ggattcggcg cagcatggcg
     2581 tcataatcct tgaggttaaa gaccacaatc acgatgtact gatcacctcc gacagtggtc
     2641 aacaccccat gggcacgaat tttatcggcg gaatttaaaa gaaacgtgtc aaataccgac
     2701 ttctcttgtt cagtttgagc caggaactgt ggggcaaggt gctgaagata gggttgaaca
     2761 atatcagcct gagccagagt cgctggctct gagagctgaa ccagagacgt ggcaatgagg
     2821 gtgtgggatg ggtccagaat cagtaactgg cgatcacgaa aacggaactc acgggtggcc
     2881 tggatgatgg cgtcagctcc tgatttgacc cccatctgct ggtgttcagc cagctcgtga
     2941 taaaacgcgt ctgaaaaaga gacggctgtt tcgtgcaacg tagcgtctac ccgctgcatg
     3001 agctggtgga caaatatttg atagaccact atcgcaaaca cccccagcac cacggcaaga
     3061 accgccaggt accagattgt cagccgcgat ctcacagaat agagcacgtc ggggaattct
     3121 ccttcgtcac agtggcgtcg ggttgtcaca ctccaaaata tacccggcgc ccctccgggt
     3181 atggatcagg tgcaatgaat gcccatcgtc caccttacgc cgaagacggt tgatatatac
     3241 ttcgatcaaa ttggaaaagg gatcgaaatt ttcatcccac acgtgttcgg caatttccgc
     3301 ccggccaatc acccggccat ggttgagtgc cagaaattca agcagcgcaa attctttggt
     3361 tgttaaatcc agggtctgac cggctcgttt tgcggtacgt gaacgggtat caatcaccag
     3421 gtcagcaatc atcagttgaa acggctgggt ttttccccgc cgacgcaaca aagctcgaat
     3481 acgggccaat aactcaccaa agtcaaatgg cttcgtcagg taatcgtcag ccccataatc
     3541 aagcccggtg attcggtttt cgacagaatc acacgcggtc aacatcagaa tcggaagttg
     3601 gactccctga gtgcgaaggt cctgacacac ttcaaatccg ttttttaccg gaatcagcac
     3661 atccagaatg atgaggtcat attgattgat ggcagcctgg tataaggccg tttcgccatc
     3721 attggccaca tccacggcat atgcctgctc acgcagcccc ttggctacga accgagcaac
     3781 ctgtggatca tcttcaacca gtaatattcg catgggtcac acaagtcttg cgccacaaga
     3841 aacacagcgt tgaattgtat ttctcacaat ctggcacgca aatttcagga tatccgcatt
     3901 gaaaggaaaa caggaggttc atctattcta ttatagagca atcaatgttc caaacctgat
     3961 aaaaataagg gaggcaacgg ttgggctgaa ggtgtcgagc gggtgatatg tattctcaat
     4021 ttttggttcg gcttcaatcc tcacacccag gtgcaagaag aagtgggcaa atttccctga
     4081 ttgaggaatt tggcgcaccg gtcagtgggc aatcttgaaa ccagcgagaa aaccagcacc
     4141 cgtgtggcgc ccggtccaca acttttttgt gaaacacttt ctcacaagtt gccacttttg
     4201 aggtaggatt tgatttcaga gccactgcct gccccaaacc tgaggtccag ccttgctgaa
     4261 gaggaactct gttttcaaga ctgtctccca atttggaatg atacattgat catttcaacg
     4321 gaagattaac ctcctcacct ggggtctcta tcctgcacct ggcaatttca aacgaaaccg
     4381 ccagttattt ctccatgccg gaaacgatgg cgtcacacag cttatgggat tggttcaacg
     4441 cgttcgctca gtcagccagc aaatggtcat ggtctatgat gaagtcaaaa ttcatcgtga
     4501 gattgaccaa ttggaaaggg aagtttccca gaatccaacc agtgtttttc acttgcagca
     4561 actgtgcaat ctctaccaga ccgctggcta cattgaaaaa gcaacctctg tactgtgcca
     4621 gcttgcttcg cttcatcgcg accggagaga acccgaatta acgctggctt ttttgcgcaa
     4681 agccgagcgg atgtgccttc ctgagcagcg gatctcattg ctgcatcaaa tcgtggttga
     4741 cttgccatct cgaatcagaa tctccagcga atgcgcacgt tgctggagat tctgattcga
     4801 gatggcaagt caaccacggc gctggaagta atcaccaaac tccccgcaat gggtgccagg
     4861 gatgcacaat accggaaaga actcacagag tatgtcaacc tgcaccgcga ggagtggact
//
LOCUS       DAOQDO010000126         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11264_length_4905_cov_2.837938, whole genome shotgun sequence.
ACCESSION   DAOQDO010000126 DAOQDO010000000
VERSION     DAOQDO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11264_length_4905_cov_2.837938"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..2381
                     /locus_tag="PKZ53_03100"
     CDS             96..2381
                     /locus_tag="PKZ53_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HNJ39450.1"
                     /translation="MNNKKLWITLGCVVAISFAVLGGVGYKTISEAPPIPIRVVSVDG
                     QEVIAPGEIQRGQAVWQSLGGQSIGSIFGHGANVAPDWSADWLHREAEYILNRWSQEA
                     NGTDYASLSAEQQAAFRERLKQELRTNTYDPVKQQITVSRVRAEAFTVLSQHYKGIFA
                     SGKNAYAIPPGALTDATRGREMSTFFWWTSWVCATNRPGEAITYTNNWPHEPLIGNVP
                     TASAVMWSIFSVILLLAGIGALVWYMGSEKKESESNEIPLRDPLLGMNVTPSQRATMK
                     YFIIVTALIVVQVIMGAITAHYGVEGNGFYGIPLAKYLPYSVARIWHLQIGLFWIATS
                     WLATGLFVAPAIGGSEPKGQKLGVDILFGALIFVVGGSLVGELLSVHQKLGDLWFWFG
                     TQGYEYVDLGRFWQVALLGGMVFWLWLVWRAIKPGLLAAKESKSLLTLFVIASIAIPL
                     FYSAGLMYGKHSHIVRAEYWRWWVVHLWVEGFFEVFATVVIAFLFTRLQLLSMKTATK
                     AVLFSTIIYLTGGIIGTFHHLYFTGTPTMVLALGATFSALEIVPLVLVGFEAWENLRL
                     SKAAEWLAAYRWVIYFFVGVAFWNLVGAGLFGFLINPPVALYYMQGLNLTPVHGHTAL
                     FGVYGMLGIGLMLFCLRAYQPEKVWHTKSLAVAFWSINLGLVLMVVLSLLPIGLLQTW
                     AAIEQGTWYARSAEFLQTGLMDKLRWLRVIGDTLFSVGVFALGWFVFRYFVDRPSAQL
                     VEDPSRRPHLPVGQAQPNTGD"
     gene            2692..3552
                     /locus_tag="PKZ53_03105"
     CDS             2692..3552
                     /locus_tag="PKZ53_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ39451.1"
                     /translation="MQPEINDLVLEAQNGSRTALESVVAYAQKYVYNLALRMLQHPMD
                     AEDATQEILIKLITHLGQFRGESSFSTWMYRIASTTLLNLVERDQMRNRVTFEGLSLR
                     LEASLTHYEQSPEEQYLAAELIEEVRRNCTLGMLMCLNREDRLALILGEVLEMSGIEA
                     ASILGVPETAYRKRLSRARQTLVAFVSRQCGIVNPDSSCRCHKHVRNKIQVGQLDPHN
                     LKLAQATDAVSALNFTQAGDRSLGDECQTMALIRSHPQYAAAHNYGEMIDRMLSAEGH
                     FGTDFFNPIN"
     gene            3589..4020
                     /locus_tag="PKZ53_03110"
     CDS             3589..4020
                     /locus_tag="PKZ53_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HNJ39452.1"
                     /translation="MSASSVVVSSTSVSRVPAHRAAGFWMVIVLIGYILVNAVRSTYN
                     PQSFAAYYGIPLADPHTTGFVFVYAIRALFLGLFGLALLIRRDYPALVWYSLIGCVMP
                     VGDAILVAVQHGGSGTVARHALTAGFLLLTGILLRRWIMSK"
     gene            complement(4268..4699)
                     /locus_tag="PKZ53_03115"
     CDS             complement(4268..4699)
                     /locus_tag="PKZ53_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39453.1"
                     /translation="MFKWLAKFFTNEDSRSPEDSISIYKNESQVQQPVPRSYTLICSS
                     CYSRCSGSNAHVIPWWNPDQKNIFTTYRCGNCWLSSLEETRQAVLNGGSEVQICFCDF
                     LTRQGLKHEARTIRDSPPEQSQALLLAILTDVQEQKVIFHP"
BASE COUNT         1149 a         1109 c         1342 g         1305 t
ORIGIN      
        1 agggtgtgac ttaggtcacc gatttttgtt cgaagagtct ctactgtgtt cacaacagaa
       61 atttcgaatt gtgaatgatg aatgagggac acactatgaa caataaaaag ctttggatca
      121 cgcttggctg tgtcgtggcg atttcgttcg cggtactggg tggtgttgga tacaaaacca
      181 tttccgaagc accgccgatt ccgattcgcg ttgtctcggt tgatggtcag gaagtcattg
      241 ctcccggaga aatccagcgc gggcaggccg tctggcaatc gctgggtggg caatcaattg
      301 gctcgatttt tggacatggg gccaatgtgg cacccgactg gtcagccgac tggctgcacc
      361 gtgaagccga atacattctc aatcgctggt cacaggaagc caatggaact gactatgcca
      421 gtttgtcagc cgaacaacag gcggcatttc gtgaacggct caagcaggaa ctccgaacta
      481 acacctatga tcccgtcaaa cagcaaatca cggtaagtcg ggttcgggct gaagcgttta
      541 cagtgttgtc ccaacactac aaaggcattt tcgccagtgg caaaaacgcc tatgccattc
      601 cacctggcgc gctgaccgat gcgaccaggg gcagggaaat gagtaccttc ttctggtgga
      661 cgtcgtgggt atgcgcgacc aatcgtccag gcgaggccat tacttacacc aacaactggc
      721 cacacgaacc gttgattggc aatgtaccaa ccgccagtgc cgtgatgtgg agcattttta
      781 gcgtcatttt attactggcc gggattggcg cgctggtctg gtatatgggt tcggagaaaa
      841 aggaatctga atcaaacgaa ataccgctcc gcgacccgtt gcttggaatg aacgtgacgc
      901 cttcacagcg tgccacgatg aaatatttca ttattgtcac ggccttgatt gtggttcagg
      961 tgattatggg agccattaca gcgcactacg gtgtcgaagg caatggtttt tatggcattc
     1021 cactggccaa atatttgccg tattcggtgg ctcggatctg gcatctccag attgggttgt
     1081 tctggattgc cacctcgtgg ctggcgaccg gattgtttgt ggcgccagcg attggcggca
     1141 gcgaacccaa aggacagaag cttggtgttg atattctgtt tggggcgctc attttcgtgg
     1201 ttggcggttc gctggtcgga gaactgttaa gtgttcatca aaagttaggt gatttgtggt
     1261 tttggtttgg aacgcaaggc tatgaatacg ttgaccttgg acgattctgg caggttgcgc
     1321 tcctgggcgg gatggtgttc tggttgtggc tggtctggcg tgcgattaaa cccggattgc
     1381 tggcagccaa agaaagcaaa tcgctgctga cgctgtttgt gattgcgagc attgcgattc
     1441 cgctctttta cagcgccggt ttgatgtatg gcaaacacag ccacatcgtg cgggccgaat
     1501 actggcgctg gtgggtggtt cacctgtggg tcgaaggctt ttttgaagtc tttgccacgg
     1561 tcgtgattgc ctttctgttt acccggcttc aattgttatc aatgaaaact gccaccaaag
     1621 cagtgttgtt ttcaaccatc atctatttga ccggaggcat tatcgggaca ttccaccatc
     1681 tgtactttac tggaacgccg acgatggtgc tggccctggg agcgaccttt agcgcgctgg
     1741 aaatcgtgcc gctggtgctg gtcggttttg aagcctggga aaacctccga ttatcaaaag
     1801 cggctgaatg gctcgcggcc tatcgctggg tgatttactt ctttgtcggt gtggcgttct
     1861 ggaatctggt cggtgccggg ttgtttggct tcctgatcaa tccgccagtt gcgctgtact
     1921 atatgcaggg attgaattta acgccagttc acggccacac ggcgctgttt ggagtctatg
     1981 gaatgctggg gattggcttg atgctgttct gcctgcgagc ctatcagccg gagaaagtct
     2041 ggcataccaa atcgctggcg gtggctttct ggtcaatcaa tctggggctg gtgttgatgg
     2101 tggtgttgag tttgcttccg attggtttgc tccagacctg ggcggcgatt gaacagggta
     2161 cctggtatgc acgctcggcg gaatttctgc aaaccggcct gatggataaa ctccgatggt
     2221 tgcgcgtaat cggagacacg ctgttttcag ttggtgtgtt tgcactcggc tggtttgtgt
     2281 tccggtactt tgtggaccgt ccatcagcac aactggtcga agacccgtcg cgccggccac
     2341 atctgccagt tggtcaggct cagccaaata cgggagactg aaagtgaggg ctgaagaaag
     2401 tgggctgagg gctaaagaca ttgagacaat cttcagccca acgtcttcta agaaaaaggt
     2461 tcagagtttc tccctcaaac agagccggac tattccttct tgagtgattg agtcgttgct
     2521 ttgtggttcg tcaaaactaa atcctgattg gcaggttcca gcttccttcc gtgattgatg
     2581 caaatcgggt tggaacgctg gaattttttt tgttcaatgg aaaaagtcat gtcacaaatg
     2641 acagagcact gtgtctaatc agcatatcac tcacaaagga agtgctctga aatgcagccg
     2701 gaaatcaatg accttgtact ggaagcacaa aatggaagcc gaacagcgct ggaatcagtg
     2761 gtggcgtatg cacaaaagta tgtgtacaac ctggccttgc gaatgttgca gcacccaatg
     2821 gatgctgagg atgcgaccca ggaaattttg atcaaattga taacccacct cggacaattt
     2881 cgcggagaaa gttcattttc aacctggatg taccgcattg cttccacaac cttattaaac
     2941 cttgttgaac gagatcagat gcgtaatcga gtgacattcg aaggtctgtc ccttcggtta
     3001 gaggccagcc tgacccacta tgaacaaagt cctgaagaac aatatctggc cgctgaactc
     3061 atcgaagagg ttcggcggaa ttgcacgctg gggatgttga tgtgtctcaa ccgtgaggat
     3121 cggctggcgt taattttagg cgaagttctg gaaatgagtg ggattgaggc ggcttcaatt
     3181 ttgggagttc cagaaactgc ttaccgaaaa cggctgtcgc gggccagaca aaccctggtg
     3241 gcttttgttt ccaggcaatg tggcatcgtc aacccggata gttcgtgtcg ctgtcacaaa
     3301 cacgttcgca acaaaatcca ggttgggcaa ctggatccgc acaacctgaa actggcccag
     3361 gcgactgatg ccgtcagtgc cttgaacttt acccaggctg gtgatcgaag cctgggtgat
     3421 gaatgccaga ccatggctct gattcggtca catccacaat atgcggcggc gcacaattac
     3481 ggcgaaatga ttgatcggat gctcagtgct gaagggcatt ttggcactga ctttttcaat
     3541 ccaatcaact aatccatttt taaatgattc atcaggaagg acttttttat gtcagcttca
     3601 tcagtcgtgg tgtcttcaac ctcagtatcc cgtgtcccgg cgcatcgtgc tgcaggtttc
     3661 tggatggtga tcgtcttgat cggctacatt ctggtaaacg cagtgcgttc aacatataac
     3721 ccgcagtctt ttgcggcgta ctatggcatt ccgctggctg acccgcatac aactggattt
     3781 gtttttgtct atgccatccg ggcgttattt ctcgggttgt ttggattggc cttattgatt
     3841 cggagagact atccggccct cgtctggtac tcattgattg ggtgtgtgat gccagtgggt
     3901 gatgccattc tggtggctgt ccagcacgga ggatcgggca cagtggcaag acatgccctg
     3961 acggctggct ttttattgct cactggaatt ctcttgcgtc ggtggataat gtccaaatga
     4021 atgaagactt gagagtgaag agtgacgaaa aaagatcagg ggttcacctt caggcgggaa
     4081 tttgtcacaa gtcacatcac tttttcttcc aaatgatcaa cacaactgtg tggccttcac
     4141 agggaatcaa gggagcaatt ttggctcttg atgcttcagt caaacaaacc tcatacactt
     4201 ttcggtggtt cagttgtgca acctgtcagt accaaaagaa cgagaaccag gatcaattga
     4261 agacgcttca tgggtgaaaa atcacttttt gttcttgaac gtcagtgaga atggcgagca
     4321 ggagtgcctg tgattgttca ggaggactgt cgcgaatggt cctcgcttca tgcttgaggc
     4381 cctgtcgagt gagaaagtca caaaaacaaa tctggacttc acttccacca ttgagaactg
     4441 cctgacgagt ttcttcaagg gaagaaagcc agcaattccc acaccgatag gtggtaaaga
     4501 tatttttttg atcgggattc caccagggga tgacgtgagc atttgagcct gaacaacgcg
     4561 aataacagga agagcagatc aaagtataag atctgggaac tggttgttga acctgggact
     4621 catttttgta aatcgaaatg gaatcttccg gagaccttga atcttcattg gtgaaaaatt
     4681 tggcaagcca tttgaacata gcgggtacat ccttttatcc tggaaaaatc caaaattcat
     4741 tggggtggat atggataacg aaccgcaaac gctggcttta aagcgtttaa atccgtaatt
     4801 caccggctca ataattgaac aagctcgatt ctgtctttgg cttcatatga ttcatcctgt
     4861 cttcggcgtg gagacccggt ccttcagggc cgggaggaaa cgccg
//
LOCUS       DAOQDO010000127         4902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11277_length_4902_cov_3.014442, whole genome shotgun sequence.
ACCESSION   DAOQDO010000127 DAOQDO010000000
VERSION     DAOQDO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11277_length_4902_cov_3.014442"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKZ53_03120"
     CDS             <1..396
                     /locus_tag="PKZ53_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ39454.1"
                     /translation="VTPLIDVLLVLIIIFMVVKHSDPHKLDSDVPEKTPENLTAQPPS
                     RLFFIECDENGKNIKLNTLPMNTPEELASRLKSELKNRLPDDRNVFISAPRNTKYEYV
                     ANLVDIVRGAEPDPQNKTVRVGLDHENKK"
     gene            498..1376
                     /locus_tag="PKZ53_03125"
     CDS             498..1376
                     /locus_tag="PKZ53_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39455.1"
                     /translation="MNRTEVISPCHRRSMARRILLSISLCFFVTSFTGCFLYQNLRAK
                     DDLNNGVRFFNASMYQEAADNFKSAYTRNPELVQAKLFYATALFKMATNATDEAAKKK
                     SIDEVLRFSKSLLEGGDSGKLNDDQKKIIHQAIISAYGLLKDDKSQREWWDRKSKLPA
                     LTTTEKSECYFQMAKIDLEQMSDLVDKYKNKPTPEEPFPAVMYKTLDQWEPKDKEEFN
                     RLALQGLKFANQSISTDADNEYGFRTKAAILDRQIALETDKDKKAEMERERNSTRDKA
                     EEINRRRAAETTGQAG"
     gene            1573..3777
                     /locus_tag="PKZ53_03130"
     CDS             1573..3777
                     /locus_tag="PKZ53_03130"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNJ39456.1"
                     /translation="MIRIEDILDKIERYNPTADLDLLRRAYLLSAKMHKGQVRKSGEP
                     YLTHPMEVANILADLKLDVVSISTGLLHDVVEDTHVTVEEIEQQFGKEIAELVDGLTK
                     IAHIENASREERQAETMRKMLLAMVSDIRVMLVKLADRLHNMRTMECMAPHKRAAKAQ
                     ETLDVYAPIAHRLGMGKIRGELEDLSFQYLYPDDFLQLKAIVEKRRETLEPYLEEVKK
                     EINRQMTLHGIRVVDIQGRIKRLYSLFLKLKRQRIPIDQVYDLMAVRVITETLPDCYA
                     ALGVIHNTWKPVPGRFKDWIAMPRNNMYQSLHTSVVGEKGQAFEIQIRTADMHKVAEE
                     GIAAHWKYKEGKASVSQKDDEMYLWLKRLVEWQQEVSDSREFMDDLKVNLYPNDVYAF
                     TPKGKVLELPRGATPIDFAYAIHSDVGQTCAGARINGHIVKLSYQIKNGDVVEILTSP
                     NARPSRDWLKMVKTGRARTRVRKYLAESERIRALEIGRKMLDREAERFKLNVKKEVTE
                     IELQRIAPEYGLQRADDIISAIGYGKLEARNVLSKLVSPQRLAEVEDASKDPTGLKQV
                     GKFIKRVLRLGTDRIQVRGVDDVMVFRAKCCNPIRGEKIIGYITRGKGVAVHQVQCPN
                     AQALMINRERIIDVEWVTDDSMSASYAVSFTVVTENRQGMLADLTAAISNIKTDIRDA
                     RASVSDDGRGRINITAEIYDVKHLERVMRAVRTVPGVVDVERRFEAGNGNQT"
     gene            4040..>4902
                     /locus_tag="PKZ53_03135"
     CDS             4040..>4902
                     /locus_tag="PKZ53_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNJ39457.1"
                     /translation="MLLYELNARLNGMTFEKITLEELIYLKKLQFDALWLMGIWQLSP
                     LGIKLSQRHASDFFGSPYAIYEYQPNPALGDVSALRALRDRVHQAGLKLIVDFVPNHL
                     ALDTPLIDQHPGYFIQSSPRMRQEYANDYYLHMSGRRLAHGKDPYFPGWEDTVQLNYA
                     SVGLRAHMRKVLKSIATVADGVRCDMAMLVLRNQVKRQWFTRLPQEKFDELMPHEFWD
                     EAIAETRKVNPEFLFIAEAYWDLEWELQQQGFDFCYDKRLYDRLEKEGAESVRLHLLA
                     DMAYQERLVRF"
BASE COUNT         1335 a         1157 c         1237 g         1173 t
ORIGIN      
        1 gtgaccccat tgattgacgt gttgctggtg ttgatcatca tttttatggt ggtcaaacac
       61 agtgatcctc acaaactgga cagcgatgtg ccagaaaaga caccagaaaa cttaacggcg
      121 cagcctccaa gtcgattgtt ttttattgag tgtgatgaaa acgggaaaaa catcaaactc
      181 aatacattgc cgatgaacac gccagaggaa ttagccagtc gtctgaaatc agaattgaaa
      241 aaccggttac ccgatgaccg gaacgtgttt atcagtgcac cacgcaacac aaaatatgag
      301 tatgtggcca atctggttga tattgttcgt ggggcagaac ctgacccgca gaacaaaacc
      361 gtccgggtgg ggttggatca cgaaaacaaa aaatagccaa tcccagtttt ttcctcagcc
      421 tgcgctcacg gatcttccaa caggccaagc catcctctaa atagcttcag aattactctt
      481 cgtcaggtgt aaaaattatg aaccgaactg aagtcatctc accgtgccat cgacggtcaa
      541 tggcccgacg cattctgttg agtataagtc tttgcttctt cgtgaccagt ttcacggggt
      601 gttttttata ccagaatttg cgtgccaaag atgacctgaa caacggtgtg cggtttttca
      661 acgccagtat gtatcaggaa gcggctgaca atttcaaatc agcctacaca cgtaatccag
      721 aattggtgca agccaagcta ttttatgcca cggcgctatt taaaatggcg accaacgcaa
      781 ccgacgaagc tgccaaaaag aaaagcattg atgaagtgct ccggttttca aaaagcctgt
      841 tggaaggtgg tgactctggg aaactcaatg atgatcaaaa gaaaatcatc caccaggcca
      901 tcatcagtgc ttatgggctc ttgaaagatg acaagagtca gagggaatgg tgggaccgga
      961 aaagtaaact gcctgccctc accacgacgg aaaagtcaga gtgctatttc caaatggcca
     1021 agattgatct ggagcaaatg tcggacctgg ttgataaata taaaaacaaa ccaactccag
     1081 aagagccttt tcctgctgtc atgtataaaa ctcttgacca gtgggaacca aaagacaaag
     1141 aagaatttaa ccgtctggcg ttgcaagggt taaagtttgc caatcagtca atttcaacgg
     1201 atgcggacaa cgaatatggg ttccggacca aggctgcgat tctggaccgc cagattgccc
     1261 ttgaaaccga taaggacaaa aaagccgaga tggagcgtga acgcaacagt acacgcgaca
     1321 aggctgaaga aatcaaccgt cgtcgggccg ctgaaacaac cggacaggct ggctagtcag
     1381 cctttgtggt cagacccaaa ccagtcgaac cccaacgagc aggtactttg tgcctgctca
     1441 tttttgtatt tgggaaccct cagccggaaa gaacccgcgt cgtaaaattt cacactcaat
     1501 tgacctggct atactttgac caaggtttgt atttcgagga agtcgcaccg ggaagcagga
     1561 gcctagacac ccatgattcg gattgaagat atcctggaca aaattgaacg ctataacccc
     1621 actgcggatc tggatcttct gcgccgggcc tatctgctct ccgcgaaaat gcacaagggg
     1681 caggtccgga aatcagggga gccatacctg acgcatccca tggaagtcgc caatattctg
     1741 gctgatctca agctggatgt ggtgtcgatt tcaactggat tgctccacga tgttgttgaa
     1801 gatacacacg tgacagttga agagattgaa caacaatttg gcaaggaaat tgccgaactg
     1861 gtggatgggc tgaccaaaat tgcgcacatt gaaaacgcat ctcgtgaaga gcgccaggca
     1921 gagacgatgc gcaaaatgct cctcgccatg gtcagtgaca tccgggtaat gctggtcaaa
     1981 ctggcagatc gtctgcacaa catgcggacc atggagtgca tggcgccgca taaacgggcg
     2041 gctaaggccc aggaaaccct cgacgtctat gccccgattg cccaccggtt agggatgggc
     2101 aagatccggg gtgaactgga agatctgagt tttcagtatt tgtatccaga tgattttctt
     2161 cagttaaaag ccattgttga aaaacgccgc gaaaccctgg aaccctatct cgaagaagtc
     2221 aaaaaagaaa tcaatcgcca gatgacgctg catgggattc gagtggttga tattcagggc
     2281 cgaatcaagc ggttatacag tctgttttta aaattgaaac gccagcgaat tccaattgac
     2341 caggtgtatg acctcatggc ggtccgggtg attaccgaga ccttacccga ttgttatgca
     2401 gcacttgggg tcattcacaa tacctggaaa ccggtgccgg gtcggtttaa agactggatt
     2461 gccatgccgc gaaataatat gtaccagtcg ctccatactt cggtggtggg cgaaaaaggt
     2521 caggcatttg aaatccaaat ccgaactgct gatatgcaca aggtcgctga ggaagggatt
     2581 gcggcccatt ggaaatataa ggaaggcaaa gccagtgttt cccaaaaaga tgatgaaatg
     2641 tacctgtggc tcaagcgact ggttgagtgg caacaggaag tttcagattc cagggaattt
     2701 atggatgacc tcaaggtcaa tctctatcca aatgacgtct atgcgtttac cccgaaagga
     2761 aaggttcttg aattaccacg gggggcaacg ccgattgatt ttgcctatgc catccatagc
     2821 gacgttggtc aaacctgtgc cggggcccgg atcaatggtc atatcgtcaa gctgtcctac
     2881 cagatcaaaa atggtgacgt cgttgaaatt ttgacctccc ccaatgcccg cccatcccgc
     2941 gactggttaa aaatggtgaa gaccggtcgt gcccggaccc gagttcgcaa atatctggcg
     3001 gaaagtgaac ggatccgggc gcttgaaatt ggccgaaaaa tgcttgaccg cgaagcggag
     3061 cggttcaagc tcaatgtgaa aaaagaagtc accgaaattg aactgcagcg aattgctccg
     3121 gaatatggcc tgcaacgagc cgatgacatc atttcagcca ttgggtacgg caagctggaa
     3181 gcacgcaatg tcttatcaaa actggtttcg ccacaacgtc tggccgaagt cgaagatgcc
     3241 tcaaaagatc caaccgggtt aaagcaggtc ggcaagttta tcaaacgagt cctgcggctc
     3301 ggcactgacc gaatccaggt ccggggcgtt gatgatgtga tggtgttccg ggcaaaatgt
     3361 tgtaatccaa tccgaggtga aaaaatcatt ggctacatca ctcggggaaa aggggttgcc
     3421 gttcatcagg ttcagtgtcc caacgcccag gctctgatga tcaaccgcga acgaattatt
     3481 gatgttgaat gggtgaccga cgattcgatg tcagcctcct atgctgtcag tttcactgtt
     3541 gtgactgaaa accggcaggg aatgctggct gatttaacgg ctgccatttc aaacatcaaa
     3601 actgatattc gtgatgcccg ggcttcggtt tcagatgatg gacggggacg catcaatatc
     3661 acggctgaga tttatgatgt gaaacacctt gagcgtgtga tgcgggctgt tcgaacggtc
     3721 ccaggcgtcg tggatgttga gcgccgcttt gaagctggga atggcaatca gacatgatga
     3781 ttcagatgac ataagcgatc agataccaac gtgaccgcct gatcagattg ttactcaatc
     3841 acgctggtat ctggtcattt ctatagtttt ttagataaat atttcctgaa tttttatttt
     3901 aggaagtctc agaaaccatt gaaaaatttg gatttatttt ccgtgtattc cgtgtattcc
     3961 gtggtttctt tatctgaaac tctatatcat cccgtcagct tgtcaccttt tcactgatca
     4021 cctgtcacct tctcactcta tgctgttata tgaactcaac gcccgtttaa atgggatgac
     4081 tttcgagaaa ataaccctcg aagaacttat ctatctcaaa aagctccagt ttgatgccct
     4141 ctggctcatg ggtatctggc aactgagccc ccttggaatt aagctttctc aacgtcacgc
     4201 ctcagacttt tttggctctc catacgccat ttatgagtat cagcccaacc cggcactggg
     4261 cgatgtttca gcgttacggg cattgcggga tcgggtacat caggctggtt tgaagctcat
     4321 tgttgatttt gtccccaacc atttggcgct ggatacgccg ttgattgatc aacaccccgg
     4381 ctatttcatc cagagcagcc cccgcatgcg gcaggaatat gccaacgatt attacctgca
     4441 catgtcgggg cgtcgcctgg ctcatgggaa agacccctat ttcccaggtt gggaagatac
     4501 cgtccaactc aactatgcga gcgtcgggtt gcgggcgcat atgcgcaaag tcttaaagtc
     4561 aattgccacg gtggcagatg gtgtccgatg tgatatggca atgctggttt tgcgaaatca
     4621 ggtcaaacgg cagtggttta cccggcttcc ccaggaaaaa tttgatgagt taatgcccca
     4681 tgaattttgg gacgaagcca ttgccgagac ccgaaaagta aacccggagt ttctttttat
     4741 cgccgaagcc tattgggatc tggaatggga gcttcagcag cagggcttcg acttctgcta
     4801 cgacaagcgc ctctacgacc gcctggaaaa agaaggcgcg gagtccgtgc ggctgcacct
     4861 gctggccgac atggcctatc aggagaggct ggtgcgcttc at
//
LOCUS       DAOQDO010000128         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11332_length_4889_cov_3.157220, whole genome shotgun sequence.
ACCESSION   DAOQDO010000128 DAOQDO010000000
VERSION     DAOQDO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11332_length_4889_cov_3.157220"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1740)
                     /locus_tag="PKZ53_03140"
     CDS             complement(268..1740)
                     /locus_tag="PKZ53_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNJ39458.1"
                     /translation="MTEKPIETQPASGDQPSASPALPDSMKQKQHSKKLPLHTRILIG
                     LAVGASLGIVVNSFANRLGPEMLQRVDWVVMNITEPVGALFLRLLLMIVVPLVFSSLV
                     VGVAGVGDIRKLGRIGLKSFAYTLVISAISVVIGLGLANTIKPGKRISPATSTQLQEK
                     YGSDAKKRVEDLQKTTAKDSAAMQVVKTIVPSNPANAVALETPNMLHLMFFALVVGVA
                     LTLVPAESAAPVLQVLEGAFAVTSKIVEMIMYLAPYAVACLLFTNTARFGLELLQALS
                     WFVVTVLLGLSLQMFGVYSLSVYFLSGLSPMDFFRRMEVVILTAFSTSSSNATLPTAL
                     RVSEENLGVPRNINTFVLTVGATANQNGTALYEGVTVLFLAQLAGVDLTLGQQLLVVY
                     LAILGGVGTAGVPSGSIPFIVAILAMIGVPPTLIAIILGVDRILDMCRTTLNVVGDIT
                     SATQGWFNIVPRDGSSEYGEENFIDKRVRVVADVNFRLKD"
     gene            complement(1918..3090)
                     /locus_tag="PKZ53_03145"
     CDS             complement(1918..3090)
                     /locus_tag="PKZ53_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39459.1"
                     /translation="MRTQLPFLRKVKLFILLCFLATGWTFVVSAYSGGPDPGYTSAPG
                     DLGTCVSCHDSFELNSGSGVVTLSGVPQMYQPGQTYPLTLTVQQAGRQRWGFQLTTVA
                     STGESVGAFVVTDPINTQLRSTTFGQLTRTYIEHTQAGTFAGTPRMGRWTFSWTAPAE
                     DRGSVSFYFAGNAANNDTTNQQDFIYSGAEIANSPSTQLAVRLLTPNGGESFEAGQPI
                     EMKWETTNASQAESHEVRLSTDGGQTFPSRIAAALSPEAQVFRWTSTPTLNTSMARVR
                     IEAYPKGGLPPVTVASAANFSIVTSNTAAITSLSPTSGQRGTKVILTLLGRGFVSGAQ
                     VSLGEGIKIKKVELLSPTELRVTVKVKATALAGTRPVEVTLPDGSKIVKESAFTVQ"
     gene            complement(3187..4452)
                     /locus_tag="PKZ53_03150"
     CDS             complement(3187..4452)
                     /locus_tag="PKZ53_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNJ39460.1"
                     /translation="MFSKHINRRNFLKQGFGFVTAGVVLPHLGLSGFAQTPSFAPSDR
                     RILVVIEFAGGNDGLNTVIPYTDPAYLKARPTIGLTDKDGILSISDRYALHPELAELK
                     PFYDAGNVAIVQSVGYPEATLSHFRSRDIWHTADPVKISGEGWLGKAAEQLYGSNVGL
                     KALSVGNGLPKTLQSPVAVVPAIVKFPRYDYQTDSRYAADGKNQEKTFTNIYGQTRTK
                     GTLDQSIAMIGLDALEGADTLQAGIAQYQSNVQYPANNPLSEGLKMLAQIITTIPESQ
                     ILYVTLGGFDNHSQQIAAADQKLSGDHATLLKYFSEGVDAFYKDLAGHGLAEQVVVMQ
                     WSEFGRRPNENGSLGTDHGTASSLFVIGNPVKGGIYGNHPNLTSLDRAGNVTFDIDFR
                     SVYGTILDGWLKVSSSQVLGATFENIGFF"
     gene            complement(4474..>4889)
                     /locus_tag="PKZ53_03155"
     CDS             complement(4474..>4889)
                     /locus_tag="PKZ53_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNJ39461.1"
                     /translation="RNVADTIALQGQDILNPPDVSGWTSGLGWINTSSMLERYNFAND
                     MLINRNREIAVMIDPKLLLPPADKQNKADLVDHFLNLMGPLEVSSKTRKELQKYLTLD
                     DSGQKGKFKLDDATIDKKVRGLLHLIMSLPEYHLN"
BASE COUNT         1212 a         1248 c         1260 g         1169 t
ORIGIN      
        1 aataatccgt cccgctccgg tttaaaggta cggtaattaa tggtttcggg ctttttcacc
       61 tcaccatagg accatgacaa aacctgttgc gggctggcca ccgagatatg gattgagtca
      121 aaattctgaa gccccgtagg ctgtccgaaa agattcatta cttcattcat attgtctgcg
      181 ctcctaagtt aacctataaa aacttttact atttctggac cccgttttca aaacggggtt
      241 acgttatttc tctgctctgc cccttaatta atccttcaac cgaaaattca catcggccac
      301 aacgcgcacg cgtttatcaa taaaattttc ttccccatac tcacttgatc catcgcgcgg
      361 tacaatatta aaccatcctt gtgttgccga agtaatatca ccaacgacat tgagggtggt
      421 ccggcacatg tccagaatgc ggtccacacc cagaataatc gcaatcaagg ttggtggcac
      481 cccaatcatg gccagaatcg ccacaataaa gggaatcgaa cctgatggaa cgccagcggt
      541 tccaacgccg cccaaaatcg ccagatacac caccagcaac tgttgcccca gcgtcagatc
      601 aacgccagcc agttgggcca gaaagagcac ggtcacgcct tcatagagcg cggtgccgtt
      661 ttgatttgcc gtggcgccaa ccgtcagtac aaatgtgttg atgttgcggg gaacgcctaa
      721 attttcctca gaaacccgca gggccgttgg gagcgtggca tttgaggatg aggtcgaaaa
      781 cgccgttaaa atcacgactt ccattcgacg aaagaaatcc atcggggaga gcccagataa
      841 aaagtacacc gaaagcgaat acaccccaaa catttgcagg ctcaggccaa gcaaaacggt
      901 caccacaaac caggagagcg cctggagtaa ctctaacccg aatcgggctg tattggtgaa
      961 aagcaggcag gccaccgcat agggtgccag atacataatc atttccacaa ttttcgaggt
     1021 caccgcgaag gcgccttcaa ggacctgaag cacgggggcg gctgattcag ccgggaccag
     1081 ggtcagggca actcccacca ccagcgcgaa aaacatcagg tggagcatgt ttggagtttc
     1141 aagggcaact gcgttggccg ggtttgaggg aacgatggtt ttgacaacct gcatggctgc
     1201 tgagtctttt gccgtcgttt tttgcaaatc ctcaactcgt tttttggcgt cagagccata
     1261 tttttcctga agctgagtgg aggtggcggg cgagatgcgt ttccctggtt tgatggtgtt
     1321 ggccagtccc aggccaatca ccaccgaaat tgccgaaata acaagcgtgt aggcaaaaga
     1381 tttcagccca atgcgtccaa gtttgcggat atcacccacg ccggcaaccc caaccaccag
     1441 tgatgaaaaa accagtggca ccacaatcat cagcagaagc cgtaaaaaca aagccccaac
     1501 cggctcagtg atattcatca cgacccaatc aacccgttgt aacatctcag ggcctaaccg
     1561 gttggcaaag ctgttgacca caattcccag cgatgccccg acggccagtc caatcagaat
     1621 tcgagtgtga agtggaagct tttttgaatg ttgtttttgc ttcattgagt caggcaaagc
     1681 gggtgaggct gagggctggt cgccagaggc tggctgagtc tcaatcggtt tttcggtcat
     1741 ggacatgtgg atgctcgcaa aaatttggtt gggttggtgg gaaaaaacgt gttgcaggaa
     1801 acaatacccg agaacagttg caaaactcca ctgcttgcca gaacgcacca acaaaaaagc
     1861 accctgcccg gagacgaacc agacagagtg ccagatcaaa aaagggagga aaaaagtcta
     1921 ttgaacggta aaagcagact ctttgacgat ttttgaccca tccggcagcg ttacttcaac
     1981 gggtcgcgtt ccagccaggg ccgtggcttt tactttgacg gtgacgcgaa gttcagttgg
     2041 gctcagcaat tctacttttt tgattttgat tccttcgcca agcgaaacct gggcaccgga
     2101 aacaaaaccc cggccaagca aggtgaggat cacttttgtc ccgcgctggc cactcgtggg
     2161 agagaggctg gtgatggctg ctgtatttga ggtcacaatc gaaaaatttg cagcgctcgc
     2221 tacggtcacc ggtggcaatc cgcctttggg ataggcttcg attcgaaccc gagccatact
     2281 ggtgttgagg gtaggtgttg aggtccaccg aaaaacctgt gcttcgggtg acagcgcggc
     2341 tgcgatccga cttgggaacg tttgtccgcc atcggttgaa agtcgaactt catggctttc
     2401 ggcctgtgaa gcattggtcg tttcccattt catttcaatc ggttgacctg cttcaaagct
     2461 ttctcctcca ttcggagtca gcagccgcac cgcaagctgg gttgacggtg agtttgcaat
     2521 ctcggcccca gaataaataa agtcctgttg attggtggtg tcgttgttgg ctgcgtttcc
     2581 ggcaaaataa aacgagactg agccgcgatc ttctgccggt gccgtccagg aaaatgtcca
     2641 gcggcccatg cgtggtgttc cagcaaatgt tccggcctgg gtatgttcga tgtaggtgcg
     2701 ggtaagctgg ccaaatgtgg tgctccgcaa ctgggtgttg attggatcag tcaccacaaa
     2761 cgccccaact gattcacccg tcgaggccac cgttgtgagt tgaaaccccc atcgctgacg
     2821 accagcctgt tgaacggtga gggtcagtgg gtaggtctgg ccgggttgat acatctgtgg
     2881 aacacccgaa agcgtcacga cgccgctccc tgaattgagt tcaaatgaat cgtggcaact
     2941 gacacacgtc ccaaggtcac ctggggcact ggtgtacccc ggatccggcc cgcctgaata
     3001 ggctgagacg acaaacgtcc agccagttgc cagaaaacac aatagaatga acagtttgac
     3061 cttccgaaga aaaggtagct gagttctcat aaatcctttt gaagtccgaa tgaaatagca
     3121 aaattgacag tcgtgtcgtc aatcgtaagg cttgctattt cgctcgttcg cggcgttgct
     3181 cgcccatcaa aaaaagccaa tgttttcaaa cgtcgcgccc agtacctggc tggaagacac
     3241 cttgagccag ccatctaaaa tggtgccgta caccgagcgg aaatcaatat cgaaggtcac
     3301 gtttcctgcc cggtcgagcg atgtgagatt gggatgattt ccgtaaatac cgcctttgac
     3361 ggggtttcca atcacaaaca gggaagaagc ggtgccgtga tcggtgccaa ggctgccgtt
     3421 ttcgtttggt cggcgaccaa attccgacca ttgcatcacc accacctgtt cagccagtcc
     3481 atgacccgcc agatccttat aaaacgcatc cacgccttca gagaaatatt tgagcagtgt
     3541 ggcatggtca ccgctgagtt tttggtccgc cgccgcaatt tgctgactgt ggttgtcaaa
     3601 tccacccagc gtgacataga ggatttgcga ttcaggaatc gtggtgatga tttgagccag
     3661 cattttcaac ccttcggata atgggttatt ggccgggtat tgcacattgg attgatactg
     3721 ggcaatccct gcctgtagcg tgtcagcccc ctcaagtgca tcaagtccga tcatcgcaat
     3781 tgattgatct agcgtgcctt tggtacgtgt ctgcccataa atattggtaa aggttttttc
     3841 ctggttttta ccgtctgccg cgtacctgga atcggtctgg tagtcatatc gtggaaattt
     3901 cacaatcgcc gggacaacgg cgaccgggct ttgcagtgtt ttcggaagcc cattgccgac
     3961 actcagggct ttgaggccaa cattactgcc atagagttgt tcggctgctt tcccaagcca
     4021 gccttcgcct gaaatcttga ctgggtctgc cgtgtgccag atatcacgcg agcgaaaatg
     4081 agacagtgtg gcttcgggat accccacgct ctgaacgatt gccacattac ccgcgtcata
     4141 aaatggtttc agttcagcca gctccgggtg gagggcatac cggtcactga ttgagagaat
     4201 cccgtcttta tcggttaatc caatggttgg tcgggctttc agataggccg gatcggtata
     4261 gggaatgact gtattgagcc catcgttgcc gccagcaaac tcgatcacaa ccagaattcg
     4321 tcggtcagat ggggcaaatg aaggcgtttg ggcaaatccg gaaagcccca gatgcggtaa
     4381 aaccaccccg gctgtcacaa agccaaaccc ctgtttaaga aaatttctgc gattgatgtg
     4441 ttttgaaaac ataggttgtt cctccctgtg accttaattg aggtgatatt ccggcaggct
     4501 cataatcaaa tgcagcaggc cgcgcacttt tttgtcaatg gtggcgtcgt cgagtttgaa
     4561 tttccctttt tggccggaat catccagggt gaggtatttc tgaagttctt ttcgggtttt
     4621 cgaagacact tcaagcggcc ccatcaaatt caaaaaatga tcaaccagat cagccttatt
     4681 ttgtttgtca gcagggggaa gcagcaattt cggatcaatc atgaccgcaa tttcacggtt
     4741 ccgattgatg agcatatcat tggcaaagtt atagcgttcg agcatagacg aggtgttgat
     4801 ccagcccagc ccgctggtcc agccgctgac gtctggcgga ttcagaatat cctgtccttg
     4861 aagcgcgatg gtatcagcga cgttgcgga
//
LOCUS       DAOQDO010000129         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11349_length_4883_cov_2.769056, whole genome shotgun sequence.
ACCESSION   DAOQDO010000129 DAOQDO010000000
VERSION     DAOQDO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11349_length_4883_cov_2.769056"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..917
                     /locus_tag="PKZ53_03160"
     CDS             3..917
                     /locus_tag="PKZ53_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39462.1"
                     /translation="MVTLVFLLIHLVPGDPVLMYLGDNARPEEVQRMRHQHGLDLPLG
                     QQYVRLWFGARSTSPGPGGAIDQNRGLLRGDFGQTFSGKSVTTQIVQRYPSTLQLALA
                     AMVVAILIAIPLGVTSAVRRGTWTDNLISAGSLLGIALPNFVLGPLAILIFSVELGWL
                     PVSGTGSIDHLVLPSVTLGAALAAILTRLVRSSVLEELNEDYVRTARAKGLPERIVLY
                     KHILKNGMIPVITIIGLQFGVLLTGAIITETIFAWPGLGELTVKSINERDYQQVQGNL
                     LIIALTYVVVNTLTDVLYRLFDPRIKVS"
     gene            complement(988..2241)
                     /locus_tag="PKZ53_03165"
     CDS             complement(988..2241)
                     /locus_tag="PKZ53_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNJ39463.1"
                     /translation="MSSLPFDSKPLEVTTAQAIILETIGFAGVERVSLTEALGRVLWE
                     EMIAPADIPPHPNSAMDGYAVRAADLAECSAESAARLKVVADLPAGYVPQAPLGPGEA
                     IRIMTGAPIPDGADSVVMVERTAKDGEFVKVFKPTRPTENIRPRGEDIRTGDVLMQPG
                     TQLQAAEIGTLAAIQRAFVTVARRPLVAILSTGDELVEVDEPLTPGKIVNSNAYSLVS
                     LVREAGGVPIHLPLVRDSREALKQAITQALAADVVVSSGGVSVGDYDYVKPVLLEMGL
                     EPKFWRVRMKPGKPLLYGILHGKPYFGLPGNPVSSMLTFLLFARPALRKLMGYPASSW
                     HLPQITAVTESDIRTKGDRPTFLRANVSYQNGQFVAKAMASQGSGVLSSMLGAEGLIF
                     LEEGTSAVAAGQTVPVLLIRNPFRF"
     gene            complement(2412..2939)
                     /locus_tag="PKZ53_03170"
     CDS             complement(2412..2939)
                     /locus_tag="PKZ53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNJ39464.1"
                     /translation="MDETVLERAKRIKVLLMDCDGVLTDGRIILLPDGQEVKAFNSQD
                     GHGLKLAQRGGIRTGVITGRKSGALEQRAREVSIEFLYQNAKDKSACLDELLQAQGFD
                     PQTIAFVGDDLPDIPVMHRVGLGIAVHNAVPEVREHAHYVTQVTGGRGAIREVVELLL
                     KAQGKWESEVSRFIA"
     gene            3018..4382
                     /locus_tag="PKZ53_03175"
     CDS             3018..4382
                     /locus_tag="PKZ53_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNJ39465.1"
                     /translation="MKTIRIASGQGFWGDWLEAPFRQVEGGPIDYLILDYLAEVTMSI
                     MQKQRARDPKAGYARDFVAMIDRVLPTLVDRNIRVIANAGGVNPRACAEAIQAVAAKH
                     GLSGRFKIGIVAGDDIMGRLDSILADGHALKNMDTGEPLSTIRPQVQSANVYFGAQPI
                     LEALQQDAQIIITGRCTDTGLTLAPLRHEFGWAADDWNKLAAGTIAGHTIECGAQASG
                     GNCLVDWETIPNLENVGFPIVEASPDGTFVITKHTGTGGRITPAVIKEQLVYEMGDPH
                     EYITPDCVADFTTIHLEADGPDRVRFFGIEGKPATEFYKVSMSFSSGYKAVGSLVYAW
                     PDAYKKAQAADRILRARLQDLGLRFEEILTEFVGVNACHGPLGGEPSSEIAEVVLRVG
                     VRSNDKASVDRFTKELAPLVLTGPPTVTGFAGGRPKVEEIVAYWPALLQKQVVTPHVE
                     VIEA"
     gene            4499..>4883
                     /locus_tag="PKZ53_03180"
     CDS             4499..>4883
                     /locus_tag="PKZ53_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNJ39466.1"
                     /translation="MRDDVRDWIALSLTTGIGPMTAQKVLKRFGFPGAVFRANRQQFE
                     GIGLSEDTREAILSQNGYREADAQLLWLQQQGASAITIQDAEYPLHLREIPDPPIVLY
                     FKGDLTSALEQPCVAIVGARHCSTYG"
BASE COUNT         1090 a         1274 c         1335 g         1184 t
ORIGIN      
        1 cggtggtcac attggtattt ttgctcatcc acctggtgcc cggtgatccg gttttaatgt
       61 acctgggcga caatgcacga cctgaagaag tgcagcgaat gcgccaccag catggtctgg
      121 atttaccact cgggcaacaa tatgtccgtc tctggtttgg tgcgcgctca acttcacctg
      181 gtccaggagg cgccattgac cagaatcggg ggttgctccg tggcgatttt gggcagacgt
      241 tttccggaaa aagcgtcacg acccaaattg tgcagcgcta tccgtccacc ttgcaattgg
      301 ccctggcggc gatggtggtg gcgatattaa ttgcgattcc attgggcgtc acatcggcag
      361 ttcgacgtgg cacctggacg gataatctga tttctgccgg gtcgttactg ggaattgccc
      421 tcccaaattt tgtcctcgga ccactcgcga ttctgatttt ttcggttgaa ctgggctggc
      481 tgccggtctc agggactgga agcattgacc atctggtgtt gccatcggtg acgcttgggg
      541 ccgccctggc tgcgatcctg acccgcctgg tgcgttcaag cgtacttgaa gaactcaacg
      601 aagattatgt ccgaaccgca cgtgccaaag ggttaccaga acgaattgtc ctctataagc
      661 acatcttgaa aaacggcatg attccggtga ttaccattat cgggttgcag tttggtgtgc
      721 tcctcactgg cgccatcatt accgaaacca tttttgcctg gccgggcctg ggtgagttga
      781 cggtcaaatc aatcaatgaa cgcgactatc aacaggtcca gggaaatttg ttgatcattg
      841 cgttgaccta tgttgtggtc aatacactga cagatgtcct gtatcggctg tttgatccac
      901 gaattaaggt ttcttaattg cgaggtcgcc agtcagggtg acaaatcgtc caaaatattt
      961 catcagaatt caaagttcaa actgaactta aaaccggaat ggattgcgaa tgagcaggac
     1021 cgggacagtc tgaccagccg caaccgctga cgtgccttct tccaggaaaa tcaacccttc
     1081 tgcccccagc attgaactga ggactcctga tccttgagaa gccattgctt tggccacgaa
     1141 ctggccgttt tggtagctga cattggctct cagaaaagtt gggcggtcac ccttggttcg
     1201 gatatcgctt tcggtcacgg ctgtaatttg aggaagatgc cagcttgatg ccggataccc
     1261 catcaatttg cggagcgccg gacgcgcaaa cagcaaaaag gtcagcatgc tcgaaaccgg
     1321 gttgcctggc agtccaaaat agggtttgcc gtgcaatatc ccgtataaca atggcttccc
     1381 tggtttcatt cgtactcgcc agaattttgg ctccagcccc atttccagca gcaccggctt
     1441 gacatagtca taatcaccaa ccgaaacccc tcctgatgaa accaccacat ctgctgccag
     1501 tgcctgagta atcgcctgtt tgagtgcttc acgactgtcg cgcaccagtg gcaaatggat
     1561 cggaacgcca ccagcttcac gcaccagcga caccagggaa taggcattgc tattgacgat
     1621 tttgcccggt gtcaatggtt catccacttc caccagttca tcacccgttg aaagaatcgc
     1681 caccagcgga cggcgtgcca ccgtgacgaa agcgcgctgg attgccgcga gggttccaat
     1741 ttcagccgct tgaagctgag tcccgggctg catcaaaaca tcgccggtgc ggatgtcttc
     1801 gccccgtgga cggatgtttt cagtggggcg cgtcggtttg aaaaccttta caaattcccc
     1861 gtctttagcg gtgcgttcaa ccatcacgac gctatcagcc ccatccggaa ttggcgcccc
     1921 ggtcataatg cgaatcgctt cgcctggtcc aagcggagct tgagggacat atccggctgg
     1981 caaatcggcg accactttca gtcgtgcggc agattcagcc gagcattctg ccagatcagc
     2041 cgcccgcacg gcatagccat ccatagcgga attgggatgt ggcggaatat cagccggagc
     2101 gatcatttct tcccagagta cacgtcccag tgcctcggtc aggctgaccc gttcaactcc
     2161 cgcaaatcca atcgtttcca ggatgatcgc ctgagcggta gtgacttcaa gtggtttgga
     2221 atcaaatgga agcgatgaca taggcaattc tccgaaagac atcgggctga aaaaccctcg
     2281 ggctcagggc ttggggctga agaaaacggg ctgaagaaag tgggtaattc agcctcaaac
     2341 ccaaagtctt cagccccgag cttgcgagac ttcagcccga tgttttcagc ccgttttctt
     2401 cagctctggt tttaagcaat aaaccgtgac acttcagatt cccacttgcc ctgggctttg
     2461 agcaggagtt cgaccacttc gcggatggcc ccgcgaccac cagtcacctg ggtcacatag
     2521 tgggcgtgct cgcgtacttc aggaactgca ttgtgaacgg caatgccaag gccaacgcgg
     2581 tgcatgacgg ggatatcggg gaggtcatca ccgacaaacg caattgtctg tgggtcaaac
     2641 ccttgtgcct gtaagagttc atccaggcag gcacttttgt ctttcgcgtt ttggtaaaga
     2701 aattcgatac tgacttcgcg ggcacgctgt tccagtgcgc cagattttcg gcccgtaatt
     2761 actccggttc ggatgccgcc gcgctgggct aatttcaaac catgaccgtc ttgtgaattg
     2821 aaagccttga cctcttgccc gtcgggaagc aagataatcc ggccatcggt gagcactcca
     2881 tcgcaatcca tgagcagcac tttgatccgc ttcgcacgtt ctaacaccgt ttcatccata
     2941 cattttcact cataaatatc aggattttcc gggaagcgaa cgaaccattc ccggtgtaga
     3001 aggactttga ttgaatcatg aaaacaatcc gcattgctag tgggcaggga ttttggggtg
     3061 actggctgga agccccattt cgtcaagttg aaggcggccc gattgactac ttgattctcg
     3121 attatctggc cgaagtcacc atgtcgatta tgcagaaaca acgcgcccgt gatcccaaag
     3181 ccgggtatgc ccgtgacttt gtcgcgatga ttgatcgggt actgccgacg ctggttgatc
     3241 gcaacattcg agtgattgcc aacgccggtg gggtgaatcc acgagcctgt gctgaggcaa
     3301 ttcaggctgt ggccgccaaa catggacttt ccgggcgctt caaaattgga attgtggccg
     3361 gtgatgacat tatgggtcgg ctggattcga ttctggctga tggtcatgcc ttaaagaata
     3421 tggatacggg cgagccgctt tccacgattc gtcctcaggt gcagagcgcc aatgtgtatt
     3481 ttggggcgca gccaattctg gaagctctcc aacaagatgc ccagattatt atcaccggtc
     3541 ggtgtacaga taccgggttg acgctggcgc cattgcgaca cgaatttggc tgggcggctg
     3601 atgattggaa caagctggct gccggaacta ttgccgggca cacgattgaa tgcggggctc
     3661 aggcatcggg cggaaactgc ctggtggatt gggagacgat tcccaatctg gaaaatgttg
     3721 gttttccaat cgttgaggct tctccggatg ggacgtttgt gattaccaaa cacaccggga
     3781 cgggtggtcg cattacgccc gctgtgatca aagaacaact ggtctatgaa atgggtgacc
     3841 cgcacgagta catcacccca gattgcgtcg ccgatttcac cacgattcat cttgaggctg
     3901 atgggccgga ccgggtgcga ttttttggaa tcgaaggcaa gccggccacc gagttttata
     3961 aggtttcgat gagtttctca tccggctata aagccgttgg atcgctggtc tatgcctggc
     4021 cggatgccta taagaaagct caggcggctg accgtatttt gcgtgcccgt ctccaggatt
     4081 taggccttcg gtttgaagaa attctgaccg agtttgtagg cgtgaatgcc tgtcatgggc
     4141 cgctgggcgg tgaaccctca tctgaaattg ctgaagttgt gcttcgggtt ggtgtccggt
     4201 caaacgacaa agccagtgtt gaccgcttta ccaaggaact ggcgccactg gtgttgactg
     4261 gcccgccaac cgtgactggt tttgccggag ggcgacctaa agttgaggaa attgtggcct
     4321 actggccagc gttgcttcaa aaacaggtgg tgacgccgca cgtggaagtc attgaggcat
     4381 aacgcttgct ctatggtgga aattgtccgg aagacggact gatggaaatt caaattcaac
     4441 ttaaattgaa tttcatcagg ctcgttttcc gcatctttcc accccgaagc gaggtgctat
     4501 gcgtgacgat gtgcgagatt ggattgccct cagtttaacc acgggcattg ggccgatgac
     4561 agcccagaaa gtattgaaac ggtttgggtt tcctggagcc gtattccggg caaatcggca
     4621 acaatttgag ggaattgggc tttcagaaga cacccgagag gcgattctca gtcagaacgg
     4681 atatcgcgaa gccgacgccc agttgctctg gctccagcag cagggtgctt cggccatcac
     4741 gattcaggat gcggagtacc ctctgcactt gcgcgaaatc ccagatcccc ctattgtgct
     4801 gtatttcaag ggtgatctga cgtcagcact ggaacaaccc tgtgtggcca ttgtcggtgc
     4861 gcggcattgt tcgacctatg gac
//
LOCUS       DAOQDO010000130         4856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11444_length_4856_cov_4.855447, whole genome shotgun sequence.
ACCESSION   DAOQDO010000130 DAOQDO010000000
VERSION     DAOQDO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11444_length_4856_cov_4.855447"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..984
                     /locus_tag="PKZ53_03185"
     CDS             109..984
                     /locus_tag="PKZ53_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39467.1"
                     /translation="MLNTLLKVKRESLPIRCEVCHQSDLFDPVQNYCARCQSTESKPS
                     IPHQFLINADTRPQPGLQLPWIFKGCARFQQALLLLYLAWIMCCFFTFLASAVLRGGE
                     PYWVPLPWSEMADFAGDSQGNVYVALRFYDTVRKYNRDGACVAAFPGVNKGSIRLEVD
                     KQDRLYIRDLGRVFVFDQHGNALNGTQPEDITQFQTRLKRFKMNEFSRSTYFCGDGTR
                     LERHWNSLTRLDSTGREVGHYHAPWYLWWATFPFPGLLAWISLAVVALRVELLDRATK
                     SRSGETQILREPDHR"
     gene            1244..2200
                     /locus_tag="PKZ53_03190"
     CDS             1244..2200
                     /locus_tag="PKZ53_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ39468.1"
                     /translation="MSRLRLHLMFALVFVMGVVGAGGSVHTTSADTPGKSFTIAVSDQ
                     KIKVKGRIAKRQGDVITLKTTDNREITVLISRTTSIKTKGGFLTSGKDFNLEALLVGL
                     RVEVEGTNDAQGQLVAQKLRFKESDLEISKTVETRVTPVEERVSRVESANEELASQVD
                     ELNNISKTLRTDIDTTRTEMVDLNKKVNERISALDEYDVKEESTVYFKVNKYDLSPEA
                     KQVLDDIAQKSTTIRGYLIEIAGYTDTTGSIQKNRDLSQKRADEVVRYMAENHNIPLR
                     RMITPFGYGSARAVGDNTTREGRDLNRRVEVRILVNRATATE"
     gene            2334..2522
                     /locus_tag="PKZ53_03195"
     CDS             2334..2522
                     /locus_tag="PKZ53_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39469.1"
                     /translation="MTFLRRVAVAELRPVLERRERSNLGSIAAPSGPFSFPSLRKAGL
                     NSRLRYAAQATAQNVNRA"
     gene            complement(2569..3105)
                     /locus_tag="PKZ53_03200"
     CDS             complement(2569..3105)
                     /locus_tag="PKZ53_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39470.1"
                     /translation="MNLRLLLTGIIVGCVILTCPVTGFAQKKKSSKASAAKTAEPPKP
                     DAAAEKRAAAQQLSVQLKGLTQFLYVFGGVTKSFEQVEEASDTEGIPATAIRKTEKTR
                     GVLAESIRNLRGNLERLENDFSQKSNLRPYFHYLIGVSDLATQAETQVNDGQLKEAGQ
                     SLLKLSNQLAETLAGLQD"
     gene            3315..4334
                     /locus_tag="PKZ53_03205"
     CDS             3315..4334
                     /locus_tag="PKZ53_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ39471.1"
                     /translation="MGCFLGVSLPVIAQDKQTEPEGDSITIEATLVTIPVIVTDAQGK
                     YIQQLKAEDFKIFDNNTAQQINFFDAADAPLHMALLIDTSYSTSGVLKEIKEAGITFL
                     RQLRPKDKAMVLSFDSEVRVLTELTDDPILMERAVAKAEIGKKYGTRLHDALAEAIQN
                     QFKSIVGRKAIVLLTDGKDLKSKLNEKAVFSIASETSTMIYTIFFMTESVVVPPDASK
                     TEPDAKAEPSRPRKVGEKQSKEEQVRTLLTDIKARREWMEQKNASARKFLDELATTTG
                     GRSFTSEVADLKQVFRLIADELRTQYTLGFYPSEDTPTSDRHVLKVEVARPDVVIRAR
                     RNYRP"
     gene            4564..>4856
                     /locus_tag="PKZ53_03210"
     CDS             4564..>4856
                     /locus_tag="PKZ53_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39472.1"
                     /translation="MRLKGLPGFAYLSACFLLTFLSVLNPTRVQAQNQYSQNKEVISD
                     ASSSFVGIADGDIDLDGLPDVAICDRGLDQVVLLLNTSSGLRSRVDISLKDFMS"
BASE COUNT         1252 a         1229 c         1138 g         1237 t
ORIGIN      
        1 cagcgtcggc ggcatcatat ttctctttat cggttacttg atatggaagc tatgcttgct
       61 tctttcacag tagccagggt cgttcagtta tttgattgag aggtacttgt gctcaatacc
      121 ttactgaaag tcaaaaggga atccctcccc attcgatgtg aagtttgcca tcagtctgac
      181 ttgtttgatc cagtccaaaa ttattgcgct cgatgccagt caacagagtc aaaaccctcg
      241 atcccacatc aatttctgat caatgctgat acccgtccgc aaccagggct acaacttccc
      301 tggatcttca aaggttgcgc cagatttcaa caagcccttt tgttgcttta tcttgcctgg
      361 attatgtgtt gctttttcac gtttctcgcc agtgcagtat tgagaggcgg agaaccatac
      421 tgggtaccgc ttccctggtc tgaaatggct gattttgccg gggattcaca ggggaatgtg
      481 tatgtagcac tcagatttta tgacacggtg cgcaaataca accgcgatgg ggcctgcgtg
      541 gcagcatttc ctggcgtcaa taagggctcg atcagactgg aagttgataa gcaggaccgg
      601 ttgtatatcc gtgatttggg acgagtcttc gtctttgacc agcatggaaa tgctttgaac
      661 ggaactcaac ctgaagacat cactcaattt caaacacgtc tcaaacgctt caagatgaat
      721 gaattcagcc gatctactta tttttgtggt gatggaacca gattagaacg gcactggaac
      781 agcctgacca gattggattc gaccgggcgt gaagtgggcc actaccacgc tccctggtat
      841 ttgtggtggg caacctttcc atttccagga ttgctggcgt ggatttcgct tgctgtggta
      901 gcgctcagag ttgaactgtt agatcgcgct accaaatctc gttcgggcga aacacaaatt
      961 ctgagagaac cggatcatcg gtaactgatt ctggactttc gaggcatttc gaaacgtcaa
     1021 ctctggattc ataacgggtt aagtgatagg aaaaattggc ttcccctgaa ccctgaaccc
     1081 tgaaccctga accctacccc acataaatcc acgctccctc ttcacattgc actcacaaac
     1141 aggtacttta gcagggttca aacgcccacc ttgtttgaac tgcccttttg atttcattga
     1201 gttttcacaa cttcttttcg atttgattca aggagacgct tttatgagcc gattgcgttt
     1261 gcatctgatg tttgcactgg tatttgtgat gggagtcgtt ggggcaggtg gatcagttca
     1321 cacgacatcc gctgacacac cagggaagag ctttacaatt gccgtcagtg atcagaagat
     1381 caaagtgaaa gggcgcatcg ccaagcgcca gggtgatgtc attacactca aaaccaccga
     1441 taaccgcgaa attaccgttt tgatttcgcg caccaccagc atcaaaacca aaggtggttt
     1501 tctgacttcg ggcaaagatt ttaatctgga agcgttactg gttggattac gggttgaggt
     1561 cgaaggcacc aatgatgcgc agggacagct tgttgctcaa aaactccgct tcaaagagtc
     1621 agaccttgag atttcgaaaa cagttgaaac tcgtgtcact ccagttgaag aacgtgtcag
     1681 tcgggtggaa tccgccaacg aggagcttgc cagtcaggta gacgaactca acaacatctc
     1741 caaaacactg cggaccgaca ttgataccac ccgcaccgaa atggttgatc tgaacaaaaa
     1801 ggtcaacgaa cgtatctcgg cgctcgatga atatgacgtc aaggaagaat ccaccgtcta
     1861 tttcaaagtc aataaatatg acctgtcgcc cgaagccaag caggtacttg acgatattgc
     1921 tcaaaaatca accactattc gagggtatct gattgaaatt gctggctata ccgacaccac
     1981 tggatcaatt cagaaaaatc gggacttgag tcaaaaacga gcggatgaag ttgtgcgcta
     2041 tatggctgaa aaccacaata ttccgctccg ccgcatgatt acgccgtttg ggtatggctc
     2101 ggcacgcgcc gttggcgaca ataccactcg cgaaggtcgc gacctcaacc ggcgcgttga
     2161 agttcgaatc cttgtcaacc gcgccaccgc gacggaatga agaaaatacc tggttcttgg
     2221 ttcttggttc ttggttcttg gttcttggtt cttggttctt ggttcttggt ttcgaaaacc
     2281 ctatcccctg ttctttcaat tggtcaccca ctcttcagaa gtaccaggtt ctgttgacat
     2341 ttcttcgccg cgtagcggtg gctgaattga gaccggtgct tgagcgccgg gaacgatcaa
     2401 atttgggatc catcgccgct ccatcagggc cattttcgtt tcccagcctt agaaaggccg
     2461 gtctcaactc acggctccgc tatgcggctc aagccacggc ccaaaatgtc aacagagcct
     2521 agattccaag caccaagtac caggcgccaa gcacattctt cattcaattc agtcctgcaa
     2581 tccggccagg gtttcggcca gttggtttga caatttgagc agcgattggc cagcttcttt
     2641 taactgacca tcattgacct gggtttcagc ctgcgttgcc agatcagaaa caccgatcag
     2701 gtagtgaaaa tatggacgca aatttgattt ctggctgaaa tcgttttcaa ggcgctccag
     2761 gttgccacgc aaattacgaa tactctcagc cagcacacca cgggttttct cagtcttgcg
     2821 aattgccgtg gctgggatgc cctcagtatc agatgcttct tcaacctgtt caaaactctt
     2881 ggtcacgcct ccgaaaacgt acaaaaactg agtcaacccc ttgagttgaa ccgaaagttg
     2941 ctgagcggca gctctttttt ctgccgcagc atctggtttg ggtggctcag cggttttcgc
     3001 cgcactggct ttggatgatt tctttttctg cgcaaatccc gtgacaggac aggtcaggat
     3061 cacgcatcca acaatgattc cggtaagcaa aagcctcaag ttcatggtat caggattcct
     3121 cctcggttgg tcgaatggac acagatgaaa ctgcttcaga aaaactggtc gggaaaattg
     3181 agtactgtgg ggcatcaatg tggttttgag cgagataaca cacccttatg agaaacgcta
     3241 ccaaaatctt ctttcgacac ccgaaaggtt cactccgatc ccacaccata tggctgggtt
     3301 ggatgatgtt cctgatgggt tgctttcttg gagtctcctt accagtcatc gcccaggaca
     3361 aacaaactga gcctgaaggt gactcgatca cgattgaagc cacgctggtc acgattccgg
     3421 tgattgtgac cgatgctcag ggaaaataca tccagcaact caaagccgaa gattttaaaa
     3481 tctttgacaa caacacggct caacaaatca atttttttga tgccgctgat gctccactcc
     3541 atatggccct gctgattgat accagctata gcaccagcgg tgtcctgaaa gaaatcaaag
     3601 aggcaggcat cacatttttg cgccagcttc gcccaaaaga caaagccatg gtcctcagtt
     3661 ttgacagcga agttcgggtc ttaaccgaat taactgatga tccaatcctg atggaacgcg
     3721 cggtggccaa agccgaaatc gggaaaaagt acggaactcg cctccacgat gccctggccg
     3781 aagcaattca gaaccagttt aagtccattg ttggccgcaa agcgattgtg ctgttgaccg
     3841 atggaaaaga cctcaaaagc aaactcaatg aaaaagcggt gttttccatt gcctccgaaa
     3901 ccagcaccat gatctacacc atctttttta tgaccgagag cgtggtggtt ccaccagatg
     3961 cctcaaaaac cgaacctgat gcaaaagcag aaccctctcg gcctcgcaaa gttggggaga
     4021 aacagtcaaa agaagagcag gtccgaaccc tgctgaccga catcaaagcc cggcgcgaat
     4081 ggatggaaca gaaaaatgcc tcggctcgca aattcctcga tgaactggcc accaccaccg
     4141 gcggacgttc atttaccagc gaagtagctg atttaaaaca ggtttttcga ttgatcgctg
     4201 atgaattgcg aacccaatac acccttgggt tttacccatc tgaagatacc ccgacctcag
     4261 accgacacgt cctcaaagtt gaagttgccc gccccgatgt cgtcatcaga gcccggcgta
     4321 attatcggcc ctgaaagcag cgaaatagtg tgcgaaaagc gaggtgtgag gaacgagtcg
     4381 tcaataaaag cctggtacgg gttatttaag ctggcgactc acttcgaccc cacgattcac
     4441 tctttcactc agaaagctat ttccctattt cgttatttcg ctataatatg gggcgttcag
     4501 ccgcttcccg aatttcattc caaaactaca catcccgttc aagttcttat cacaggaaaa
     4561 actatgcgtc tcaaagggtt accagggttt gcctacctgt ctgcatgctt tctgctcaca
     4621 tttttgagcg ttctcaaccc cacacgtgtc caggcccaaa atcagtatag ccaaaacaaa
     4681 gaggtgattt cggatgcttc gagcagtttc gtcggtatcg ccgatggtga tattgatctg
     4741 gacggcctgc ccgatgtcgc catttgtgac cgtgggctgg accaggtagt gctcttgctc
     4801 aacacctcat cgggtttgag aagccgggtt gatatttcac tcaaagattt tatgtc
//
LOCUS       DAOQDO010000131         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11537_length_4833_cov_2.786522, whole genome shotgun sequence.
ACCESSION   DAOQDO010000131 DAOQDO010000000
VERSION     DAOQDO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_4833_cov_2.786522"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..3708
                     /locus_tag="PKZ53_03215"
     CDS             280..3708
                     /locus_tag="PKZ53_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNJ39473.1"
                     /translation="MVFRNQKIPGALARQHWPVRMLWLGFWVFGLVFQPLMAQISQGK
                     LNPTVPEIEDQIKQVENRPGSDTNRNKAIETYRMALNQLQLAEEWTQKAQSFVREQQA
                     APQLLQALQKELDSPLPSLTYTLSNQPTTDELDQVLTKATTDLNSNQDIVNELARKLI
                     ALSERRRLLPGLMMDAKSRAGKIKTEFATRPLDASNDQATALRTLNAATEKAILAEIE
                     ALEDELAGLDQAEKIAKLRYDLETRKITRLDQQIKAWREKLTTSRRTEAELTLQQTQA
                     DLQKAVELHPGLRPLAEANVALAGLRTGPESAAVAGEQTRRLLDQFKQKRELLTADFD
                     RLVSRVATTGLTNSVGMLLLRKRAELPNVNQLEKNIQARQALISSVQTQIFECSEQTI
                     PTTDIEPLVQATLLKLDPLPLKPQQEAIEATIRSLLLTKSRLLDQVTQDYSSYFDELV
                     NLDSEERGLILDTRRYSDYISENVLWVRGVPPLKAGDLLKAASLIRSKLTLQFWKQEG
                     GTLWKGFLQDPFTSLFIIVGIALLLFVQTFLGRKVRLGEQDSAAWCFNTPFADIVGLY
                     FLVLLNALIWPVIIFSIALRLSLFAQGSPILTNLAFGVGAIVGPLFLCQLIRNYACKS
                     GLGEIHLGWSPERSALIRTQMRRIFAVVLPAFFLAAALGMNSIEVTDDTLGRLPFLVA
                     LVVLAASLYQIFHPAGFPDETDADPSKWEVWKKWLVCWFVVSIPLGIGLVAALGFTYT
                     AWNLSRKAELTLAVVLLVSVLRALLRTWSSQRRLEKPPSCDASSTPPQERSVKPEENN
                     VSPLVITDQIYDLIWALTVLVALVLVGYIWTEFFPIFKTLNRIELWSYTESISQSITA
                     SDGSQTSKMVENRHPTTILDLLIAVAIAVVTYSAVQNVPTLLKLTLLSKLPLDAGVKY
                     AISAVTSYLLTLTGVIFSLSQIGVTWSKVQWLAAAFSVGLGFGLQEIFANFVSGIILL
                     FERPIRVGDIVTVGTVTGTVTRIGARATFVLDFDRKELVIPNKSFITGQLVNWTLSDD
                     IVRVQIPINVAYGSNVELVEQLLFQTADELPDILKDPPPRVIFTNFGDSHLEFMLYVY
                     LESANAILTTKHLLHTKINRLFREAGVEIPFPQRDIHIRTVPPGQQVARSEEAK"
     gene            4024..>4833
                     /locus_tag="PKZ53_03220"
     CDS             4024..>4833
                     /locus_tag="PKZ53_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ39474.1"
                     /translation="MTHILKSRRQWLSMLGGGLISTMATGCRAADLNHTVSPSTTSSV
                     MAEPRTVNPNDILKRTIPSTGEKLPVIGLGTWQTFDVGTSASERAPLGEVLQLLVKTG
                     GSMIDSSPMYGQSESVVGDLTEQLQIRDRLFLATKVWTQGQQPGIDQMEQSARLLKAK
                     TIDLMQIHNLIDWQTHLRTLRDWKARGKIRYLGITHYTVSSYPDLERIIQTEPIDFVQ
                     FNYSITTRDAEARLLPLAAEKGVADIINRALESGSLFQRVKGKSLPPWASEL"
BASE COUNT         1211 a         1270 c         1169 g         1183 t
ORIGIN      
        1 attgagaatg aagaatgaag aatgaagaat gaagaatgaa gaatgaagaa tgaaaatgaa
       61 gaatgactta aactcattga cgtcatatag ttaatgattt tctgatacga tgggctcatt
      121 tcaacacaat tcaactccaa attatgtggc gccaggctca ggccgccaga cctgaaatcc
      181 agtttatgct ctcagcactt tccccagtta acccttttag attcagcttc ttatgtgact
      241 gtcaacgcgt tgacacttag cgctcaaagc gaggtatgtg tggtgtttcg aaatcagaaa
      301 attccaggcg cacttgcccg gcagcactgg ccagttcgta tgctgtggct gggattctgg
      361 gtgtttggat tggtgtttca gccactgatg gctcaaatat cgcagggaaa actcaatcca
      421 accgtgcctg agattgaaga tcaaatcaag caggtagaaa accgtcccgg atcggataca
      481 aatcgaaaca aagcgattga aacctatcgc atggcactca accagcttca actggctgag
      541 gagtggacgc aaaaagccca gtcgtttgtg cgggaacaac aggcagcgcc ccagctactg
      601 caggcgcttc aaaaagaact tgatagtccg ttgccctcgc tgacctatac cctgtcaaac
      661 cagcccacca ctgacgagct ggatcaggta ctgaccaaag ccaccactga tctgaattca
      721 aaccaggaca tcgtcaatga actcgcccga aaattgattg ccctttcaga acggcggcgg
      781 ttgttaccgg gcctgatgat ggatgccaaa tcacgggctg gcaaaataaa aaccgagttt
      841 gccacgcgac cacttgacgc cagcaacgat caggccacgg ccttgcgcac actcaatgcg
      901 gctacggaaa aggctattct ggctgaaatc gaagccctcg aagacgagct ggctggatta
      961 gaccaggccg aaaagattgc gaaacttcgc tatgaccttg aaacccgaaa aatcacccgc
     1021 cttgaccagc aaatcaaagc ctggcgtgaa aaactcacca ccagccgtcg aaccgaagcc
     1081 gaactcaccc tccagcaaac ccaggctgat ttgcaaaaag cggttgaatt gcaccctggg
     1141 ctccggccac tggctgaggc gaacgtcgct ctggctggct tacgaaccgg accagagagc
     1201 gccgcggtgg cgggtgaaca aacccggcga ctccttgatc aattcaagca aaaacgcgaa
     1261 ctcctgacgg cagattttga ccggctggtc agtcgggtgg cgaccaccgg gttgaccaat
     1321 tcggtcggca tgctgttgct gcgcaaacga gcggaactgc ccaatgtcaa tcaactggaa
     1381 aaaaacatcc aggcccggca ggcgttgatt tcctcagtcc aaacccaaat ttttgaatgc
     1441 agcgagcaaa ccattccaac caccgacatt gagccgctgg ttcaagctac actcctcaaa
     1501 cttgatccac tgccgctcaa accacagcaa gaagccatcg aagccacaat ccggagtttg
     1561 ctgctgacca aatcccggct gctggatcag gtcacccagg actacagttc ctattttgat
     1621 gaactggtca atctcgactc tgaagaacgc gggttgatcc tcgatacccg ccggtatagc
     1681 gattatatca gtgaaaatgt gctctgggtt cgaggggttc caccgctcaa agctggcgat
     1741 ttgctcaaag ccgccagcct cattcgaagc aagctcacgc tacagttttg gaaacaagaa
     1801 ggaggcacgt tgtggaaagg gtttttgcaa gacccgttta ccagcctctt tatcattgtt
     1861 gggattgctt tactcctctt tgttcagacc tttttagggc gcaaggtccg cctgggcgaa
     1921 caggattcgg cagcctggtg ctttaacacc ccgtttgccg atatcgttgg attgtatttt
     1981 ttggtgctgc tcaatgccct gatctggcca gtgattattt tctcgattgc cctccgactc
     2041 agcctctttg cccaggggtc acccattttg accaatcttg cctttggcgt tggcgcaatt
     2101 gtcggcccgc tctttctgtg tcagttgatt cgcaattacg cctgtaaatc tggactggga
     2161 gaaattcatc ttggctggtc accagaacga agcgcgctga ttcgcaccca gatgcgccgc
     2221 atctttgcag tcgttcttcc ggcattcttc ctggctgccg cactgggcat gaacagtatc
     2281 gaagtgaccg acgataccct gggacgattg ccttttctag tggcactggt tgttttggca
     2341 gcttcgctct atcagatttt tcacccagcc ggattccccg atgaaactga cgctgacccc
     2401 agtaagtggg aagtatggaa aaaatggctg gtgtgctggt ttgtggtttc gattccgctc
     2461 gggatcggcc tggtggcagc ccttgggttt acctataccg cctggaattt aagccgcaaa
     2521 gccgagttaa cgctggccgt ggtgttgctg gtgagtgtct tgcgggcctt gttgcgcacc
     2581 tggagttcac aacgaagact tgaaaaaccg ccctcctgcg acgcatcctc cacacctcca
     2641 caggaacgct cggtcaagcc cgaagaaaat aatgtttcac cactggtcat caccgaccag
     2701 atttacgatc tgatttgggc actgacagtg ttggtcgcgc ttgtgttggt cgggtatatc
     2761 tggaccgagt ttttccccat tttcaaaacc ctcaaccgga tcgaattatg gtcttatact
     2821 gaatccatca gtcagtcaat cacggcatcg gatggctcac aaacctcaaa aatggtcgaa
     2881 aaccgccatc caaccaccat cctggatttg ttgattgccg tggccatagc ggtcgtgacc
     2941 tatagtgcgg tgcaaaatgt gccaaccttg ttaaagctca cgctgttgag caaattaccg
     3001 cttgatgctg gtgtcaaata tgccatctca gccgtcacca gttacctgct caccctgact
     3061 ggtgtcattt tctcactcag tcaaattggc gtgacctggt caaaagttca gtggctggca
     3121 gcagccttct cggttgggct tggctttgga ctccaggaaa tttttgcgaa ttttgtctct
     3181 ggtatcattt tgctctttga gcgaccaatt cgtgtcggag acatcgtcac ggtcggaacc
     3241 gtcaccggca cagtgacccg catcggcgcc cgagccacgt ttgtgcttga ttttgaccgc
     3301 aaagaactgg tgattccaaa caaaagcttt attactggac aactcgtcaa ctggaccctt
     3361 tcagatgata tcgtccgggt tcaaattcca atcaatgttg cctatggttc aaatgttgaa
     3421 ctggttgaac agttgttgtt ccagaccgcc gatgaacttc cggatatttt gaaagatccg
     3481 ccgccacgag tcatctttac caactttggc gacagtcacc tggaattcat gctctatgtt
     3541 taccttgaat cagccaacgc cattctgacc acaaagcatt tgcttcatac caaaatcaac
     3601 cggctctttc gcgaagccgg ggttgaaatt ccctttcccc agcgagatat tcacattcgg
     3661 acagttccgc caggacagca ggtggccagg agcgaagaag cgaagtaacg acgagtcaac
     3721 ccatcgagca gcgatcaact gcttcaccac agaggaacac agaggccgct cacttcctca
     3781 aaaacctctg tggccctctg tgctctctgt ggttcaaaaa tgatgctgac ggttcattgt
     3841 gttcggggtg atgggggtac ttcaggttga tattttggaa atcgaaaaaa tttggggaat
     3901 gacaaaaccc ggaaccccga acccaatctt tttacagaaa aactgttttc tgattttcat
     3961 ttttgatctt tgatgacagt tcttctccag gccaatttct taccaaagcg agccgattgg
     4021 tcaatgacac atattttgaa gagccgtcgc caatggttgt cgatgctcgg tggtggactg
     4081 atctcaacaa tggccaccgg atgtcgggct gctgatctca accataccgt atcaccatca
     4141 actacatctt cagttatggc cgaaccacgt accgtcaatc ccaatgacat tctcaaaaga
     4201 acaattcctt ccactggtga aaagcttcct gtgattgggc ttggaacctg gcaaaccttt
     4261 gatgttggta cgtcagccag tgaacgggca ccactcgggg aggtgctcca actgctggtg
     4321 aaaactggtg gttcgatgat tgattcatcc ccgatgtatg gtcaatctga aagcgtcgtc
     4381 ggtgatttaa ccgaacagtt acagattcgc gaccggttgt ttctggccac caaagtctgg
     4441 acccagggcc agcaaccagg aattgaccag atggagcaat ctgcccgttt actcaaagcc
     4501 aaaaccattg atttgatgca aatccataat ttgattgact ggcaaaccca tctgcggacg
     4561 ctccgggatt ggaaagccag agggaaaatc cggtatctgg gaattaccca ctacacggtt
     4621 tcttcctacc cggatttgga acgcattatc caaactgaac ccattgattt tgttcagttt
     4681 aactattcaa tcacaaccag ggacgccgaa gcgcggctgc tgcccctggc ggctgaaaaa
     4741 ggtgtggcgg acatcatcaa ccgggcgctt gaaagtggat ctttgtttca gagggtcaag
     4801 ggaaagtcac tacctccgtg ggccagtgaa ctt
//
LOCUS       DAOQDO010000132         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11564_length_4826_cov_3.067072, whole genome shotgun sequence.
ACCESSION   DAOQDO010000132 DAOQDO010000000
VERSION     DAOQDO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11564_length_4826_cov_3.067072"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..>4826)
                     /locus_tag="PKZ53_03225"
     CDS             complement(346..>4826)
                     /locus_tag="PKZ53_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39475.1"
                     /translation="DTTVNGGFLLELTGRERNLSADGRFVVYRSTAPNLVAGQVDQAT
                     TSDIFVFDRVAGTNRLVSGVNGSATVTGNDFSTRPAISGNGQFIAFLSKATNLVPGQV
                     DTNNELDAFVFDRVNGTTRLASGVNGSTTQTGNDGINGQFRLDISGDGQFIVFSSDAE
                     NLVPGQTDNNAVDVFVFDRVNGTTRLVSGVNGSATQAGNGGSLNGVINQTDGRFIAFT
                     SFATDLVPGQVDASANSADVFVFDQVNGTTRLVSGVAGSTTDVANESSFIEAISGNGD
                     TVVFSSNATNVVTGQVDANNTADLFVFDRVNGTSELVSGAAASATVTGNGFSASNIST
                     TENGNVIAFLSQATNLVPGQVDINQDADVFVFDRIANTTRLISHQVGSATTAADDPSN
                     TALISADGSAVVFSSEATNLIAGQNDQLDSEDIFVFSLANNTTQLFSGDNGSALTGNR
                     TGFSTKPVASQDGSIIAFESVASNLVPNDFNQGLDVMVVQTSNRQTQLATLRAGVPPA
                     TPFGTSDIENVSSNGRFIVFRSDATNLVPGQNDTNLQEDLFVFDTQSGTTRLVTGVDG
                     SATTTVADFEVDHSFGSISADGRLITFRSNAVNLIAGQVDEPFTNDIFVFDQVSGTTS
                     LISRVAGTTNTATGGALSNPTISADGNFIVFESGATNLVAGVNDANQTTDIFLFNRQA
                     GTIQLVTAQAGSSTTTANGNSRGPSISADGRYIAYESQATNLVAGIDDLNSNVDVFVF
                     DRLTGTTQLVSQVFGGFITANQRSAGPTVSADGNFIVFESQATNLVENQVDSNNFNQD
                     VFVFDRITRVNHLVSRAAGTVNTTGSDGSQLSDGGKLNISANGRFVAYASKATNLVTG
                     FVDGNGASFTDVFVFDLTTGLNQLVSGAGTSAVNGADELSTEPALSFDGRIIVFESRA
                     TNQVENQVKANADSDADVVVFDQIAGTEELVSRQLGTQTTTGNEDSVQVVVSGSGSLI
                     AFESNASDLIEADFNDQTDVFYVEQTSCDASIGAPSPLTACQGTATGSLLGNVPPDGS
                     TGLWDDGGAGGTFIPDANAPDASYAPPFTFTGAITLTWTISGGGCGTSSASIDVNVLP
                     NLVVNAGPDQVVCIGQTVQLAGSFVGDAAFAPVWTTGGTGTFSPDANTLNAIYIPSAL
                     DLEDGLVLLTLTSPNAPADICGNSVDEVQINFEACVELPVLLVADTNNHRVQRFDGAT
                     WSVILGNGVRGSSLTQASAPEAVTAHFDGSTIYVADTGNNRILRSTNSGASWSVLAAS
                     GVGLNQVKAPKGLALDLQGNLYVADTGNQRVLRFNNGVPGAAVVLATIGSGGGQVRNP
                     NGLAVDPENNLYIADRDNNRILILSQANTVAVANTSTVFVSGPGAGLTQVRNPEGVAV
                     DNNGNLFVADTGNHRVLQFSTLAPRPGTVRLAAAPGTAITSTGSSLGQTNAPEGVTVS
                     QFTAGAFAGQSLLVIGDTGNNRIQGRPVAGGTFQLVGVPNNIGSGAGQFRNPSKIR"
BASE COUNT         1180 a         1357 c         1216 g         1073 t
ORIGIN      
        1 aactttccaa tttcgtccca acagcgacga attacggtgg cgcatgatct taccttgaaa
       61 cgcaggcggc tgccgggacc gggatttggt cgttggtcac gcaagcctgc gtatggtgtt
      121 gtttggattg tggttgagag cgttgatgag gaatgagcgc cctggactgc tttatcgttc
      181 ttatgggccg tagccaggca gtatcccacg gtccgatgaa acctgcaaga tggctgagta
      241 acaaggacaa aatgacaaaa tgacaggtga ccagatgaca atcgttaaag tcaccctggt
      301 cacctgtcac ccggtcagat gtcacctggt catctggtca gcttgtcacc gaattttgga
      361 cgggttgcga aattgcccgg caccgctgcc gatgttattg ggaacgccga caagctggaa
      421 ggtgccgcca gcaactggac ggccctgaat ccggttgttt ccggtatcgc cgatgacgag
      481 cagggattgc ccagcaaatg caccagcggt gaactgactg accgtaactc cttcgggagc
      541 attggtttgc cccaatgaac tgcctgtcga agtaatggcc gtaccaggtg ctgccgccag
      601 gcgaaccgtg cccggtctgg gggcaagggt tgaaaactgc agcacccgat gatttccagt
      661 atcagccaca aacaggttgc cattgttatc aaccgctacg ccttctggat ttcggacctg
      721 agtcaacccg gcaccggggc cactcacaaa aacggttgag gtattggcca ccgccactgt
      781 gttggcctga gagagaatta aaatgcggtt gttgtcacgg tcggcaatat agagattgtt
      841 ttctggatca accgccagcc catttggatt tctgacctgc ccgccaccgc tcccaattgt
      901 tgccagcaca acagcagcgc cgggaacacc attgttaaag cgcaataccc gctggttgcc
      961 ggtatcggcc acatagaggt tgccctgcag atcaagcgcc agtcctttcg gtgctttgac
     1021 ctggttaagg ccaacaccgc tggcggccag caccgaccag cttgcgccac tgttggtcga
     1081 gcgcaaaatg cggttgttcc cagtatcagc cacataaata gtggaaccat cgaaatgagc
     1141 agttaccgct tcaggagcac tggcttgcgt taatgagctt ccccgcacgc cattgccaag
     1201 gataacgctc caggtcgcgc catcaaaccg ctgaacccga tgattgttgg tatccgccac
     1261 caacagcact ggcaattcaa cgcaggcttc aaaattaatt tgcacctcat ccacgctatt
     1321 gccacagatg tcagccggtg cgtttggact ggttaaggtc agaaggacaa gtccatcctc
     1381 cagatcaagc gcagatggaa tgtaaatcgc attcaaggta ttggcgtcag gcgagaatgt
     1441 cccggtgcct ccggttgtcc aaaccggagc aaacgcggca tctccgacaa aactcccggc
     1501 aagctggacg gtttgcccaa tgcacaccac ctgatccgga ccggcattga ccaccaggtt
     1561 gggcaacaca ttcacgtcaa tgctggctga agaggtacca catccgccgc ctgaaatcgt
     1621 ccaggtcagc gtgattgctc cggtaaaggt gaacggtggg gcataactgg cgtcgggggc
     1681 attcgcatct ggaataaagg taccgccggc gccaccatca tcccagagcc cggttgagcc
     1741 gtcgggtgga acattcccaa gcaatgaccc ggttgccgtc ccctggcagg ccgtcagtgg
     1801 ggatggtgcc ccaatgctgg catcacaact ggtctgttcg acataaaaca cgtctgtctg
     1861 gtcattaaaa tcggcttcga tcaagtctga agcgttactt tcaaaggcaa tcagcgaccc
     1921 acttccactc accacgacct ggactgagtc ttcatttcca gttgtggttt gcgttccgag
     1981 ctggcggctg accagttctt ctgtcccagc aatctggtca aatacaacca catcagcatc
     2041 tgaatcagca ttggctttga cctgattttc aacctgattg gtcgctcgtg attcaaaaac
     2101 aatgatccgg ccatcaaaac tcaaggctgg ctcggtggaa agctcatcgg caccattcac
     2161 agcggaggtc ccagccccgc tcaccagttg attgagtcca gtggtcaggt caaaaacaaa
     2221 cacatctgtg aaactggcgc cattaccatc cacaaaacca gtcaccagat tggtggcctt
     2281 tgacgcatag gccacaaatc gtccattggc gctgatgttc aattttccac cgtctgacaa
     2341 ttgcgaacca tcagacccgg tcgtgttgac cgttccggcg gcacggctga cgagatggtt
     2401 caccctcgta attcgatcaa atacaaacac gtcctgatta aaattattgc tatcaacctg
     2461 attctcaacc agattggtgg cctggctttc aaaaacaata aagttcccat cagcactcac
     2521 cgttggcccg gcggaacgct ggttggccgt aataaaccct ccaaatacct ggctcaccag
     2581 ttgggtcgta ccagtcaacc gatcaaagac aaacacatca acatttgaat tgagatcgtc
     2641 aattccggca accaggttag tggcctggct ttcatacgca atatatcgtc catccgcgct
     2701 gatggatggg cctctggaat tcccattggc cgtggtggtt gagcttccag cctgagcggt
     2761 caccagttgt atcgttccgg cttgccggtt gaagagaaaa atatccgtgg tctgattggc
     2821 gtcatttacg ccagcgacca gattggtggc accagactca aacacgatga aattgccatc
     2881 ggcactgatg gtcgggttgc tcagggcccc gccagtcgcg gtattggtgg ttccggccac
     2941 acgagaaatg agactcgttg ttccgctgac ctgatcaaag acaaagatat cgttggtaaa
     3001 tggctcatcc acctgcccgg caatgagatt gaccgcatta ctgcgaaagg taatcaaccg
     3061 accgtcggca ctgatggagc caaagctatg atcaacctcg aaatcagcca ctgtggtcgt
     3121 ggctgaacca tcaacccccg tcaccaggcg cgtggtgcca gactgggtgt caaatacaaa
     3181 caggtcttcc tgaagattgg tgtcattttg cccagggaca agattggtgg catcgctgcg
     3241 aaacacaata aaccgcccat tgctgctcac attttcaata tctgacgtgc caaagggggt
     3301 ggcaggtggc accccggcgc gcagggttgc cagttgggtc tggcgatttg aagtctggac
     3361 aaccatcaca tccagcccct gattgaaatc atttggcacc agattggaag ccacactttc
     3421 aaacgcgatg atactcccat cctggctcgc gacgggtttg gtcgagaacc cggtccgatt
     3481 gccggtcagg gctgatccat tgtcgccgga aaagagttgc gttgtgttat tggccagtga
     3541 gaaaacaaaa atatcttcgc tatcaagttg atcattctga ccggcaatca ggttggttgc
     3601 ttcgcttgag aacacaactg cgctcccatc ggcgctgatc agggccgtat ttgacggatc
     3661 atccgcagcc gtagtggccg aaccgacctg atgactgatc aggcgagtgg tattggcaat
     3721 tcgatcaaat acaaagacat ccgcgtcttg attgatatcc acctgccccg gcaccaaatt
     3781 ggtggcctgg ctcaaaaaag caatcacatt gccattttcg gtggttgaaa tgttactggc
     3841 tgaaaatccg tttccggtca ccgtggctga ggccgcagcc ccactgacca gttccgaagt
     3901 tccattcacg cgatcaaaca caaaaaggtc agccgtgtta tttgcatcaa cctgaccagt
     3961 caccacgttg gtggcattgc ttgaaaaaac aaccgtatcg ccattgccgc tgatggcttc
     4021 gataaatgaa ctctcattgg caacgtccgt ggttgaacca gccacaccac tcaccagccg
     4081 ggtggtgcca ttgacctgat caaaaacaaa cacgtcggct gaattcgcac tggcgtcaac
     4141 ctgacccggt accaggtcag tcgcaaaact ggtaaaggca ataaaccgtc catcagtttg
     4201 attgatcact ccgttcaacg aaccgccgtt cccagcctgg gttgccgaac cattcacccc
     4261 actgaccaga cgggtcgttc cattcacccg gtcaaagaca aaaacatcaa cggcattatt
     4321 atctgtctga ccaggaacca aattttcagc atcgctcgaa aagacgataa actgtccatc
     4381 tccactgata tccagccgaa actgaccgtt gatcccatcg ttgccggtct gggtggttga
     4441 gccattgacg ccacttgcca gccgggtcgt accgttcacc cggtcaaaca caaaggcatc
     4501 caactcattg ttggtatcaa cctggccggg aaccaggttg gtggccttgc tgagaaaggc
     4561 aataaattgt ccattgccac tgatggccgg cctggtggaa aaatcattgc cggtcacagt
     4621 tgccgaacca ttgaccccgc tcaccagccg gttggtgcca gccacccgat caaaaacaaa
     4681 gatatcagag gttgtcgcct gatcaacctg accagcaacc agatttgggg ctgtgctccg
     4741 atagaccaca aaccggccat cggcactcag gttccgctcc cgtccagtca attcaagcag
     4801 aaaaccacca ttaaccgtgg tgtctc
//
LOCUS       DAOQDO010000133         4824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11576_length_4824_cov_3.591948, whole genome shotgun sequence.
ACCESSION   DAOQDO010000133 DAOQDO010000000
VERSION     DAOQDO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11576_length_4824_cov_3.591948"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PKZ53_03230"
     CDS             <1..905
                     /locus_tag="PKZ53_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39476.1"
                     /translation="AEEARKAEEARRKAEEESRRAEEARKAEEARRKAEEARKADEAR
                     KAEEARRKAEEEARRKAAEEEARRKAAEEEARRKAEEDARRRADEARRAEEARKAEEA
                     RRAEEARRAAEAAKKAQEAQFKPPVFELPPEEPPKPKAPEPEQVFFVPPGGAAPEPIQ
                     PPKPQPSPIAAQGTIPLTSFTPPAGYQSAPPPPSSTAPGVFKYDPAKYKGSEADHKTA
                     QRVARTIVSDIKIYNEKKVVEGRKSKNLYDLLKKEFDHGLKTYEDRIGEVVRAESTYL
                     YDTIVDKLCDGDPSCLGPNFPKNY"
     gene            1123..3090
                     /locus_tag="PKZ53_03235"
     CDS             1123..3090
                     /locus_tag="PKZ53_03235"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNJ39477.1"
                     /translation="MKNRTCTWWWLYGLGLIVLSGYHSISARETPLSDRVVVYQIEAV
                     LDPVAKHIQGKETLRWRNPSDAPVTELQFHLYLNAFRDQKSTFMVESGGQLRGDYLKS
                     GKFGSIDVLSLRTSDGEDLLPRAEFIAPDDSNRDDRTVWRIPLTRPVGPHQEITLSIE
                     FLSKLPRVFARTGYLGNYFLVAQWFPKLGVYEPAGLRRRTTSGWNCHQFHANTEFYAD
                     FGTYDVTFVVPETYKVGATGVLQSKESRPGNQMAYRYVQADVHDFAWTASPDFLEFED
                     TFRHPDLPEVKLKLLLQPSHLAQKDRHLAAVKNSLQYCGLTYGAYPYQTLTIVDPAYQ
                     ADGSGGMEYPTLITCGTLYRVMDDEALFEASPEVVTTHEFAHQYWYGMVASNEFEESW
                     MDEGFTSFTERKLMDAFYKDQTAIWFRLVGQPIMRLPIRLDVWSFNRVTLTPATVKCD
                     PLMTQSWKYKTDDQCEVSYYFNSYPRPALTLEMLERHLGTPTMNRILKTYFERWRFKH
                     PTAQDFFDVASEVAGQNLDWFFQQYFRDTVVLDYAIQSAKTTGTGTQFVTEVTVARKG
                     EAVMPVEVEFRFTDGSRLRETWDGKAAEKRFAVERSAALQSVVVDPDHKLMLDVDWFN
                     NSYVVNPSQVGTVNLVSRLIFLLQEALCFAL"
     gene            3075..4112
                     /locus_tag="PKZ53_03240"
     CDS             3075..4112
                     /locus_tag="PKZ53_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39478.1"
                     /translation="MFRIVINSLVQAARHWRLILMLMLATWCASTLVAFPIYGLLNRL
                     NGNSEMADRLLQPDLDAAWLAEVAHFPELVSQYGAWFIGGLMVVGLGYWIVGIYLTGG
                     LIGLFVDPEKQSSGMENQGSGFRVPGSGSDPGNSHQTMSSEPLIPGEMVNTNNWESEP
                     GTRNPEPGTRNPGFLANCAASFGTFMRVALISVFLHSLLICLGFLGWRLISQYAETSA
                     NEPQVRFLRMMVYLLAILFLVVIHLFLDYLKIRIVLTRQAWVTRQILPTLILIVRHWQ
                     KLIGVFGLMLVITLVVQVLLAQISFSISPNNWFLVGLGGVTHQVVIMTRWWLKLGWYA
                     SEVNAVQSTGA"
     gene            4267..>4824
                     /locus_tag="PKZ53_03245"
     CDS             4267..>4824
                     /locus_tag="PKZ53_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ39479.1"
                     /translation="MRCSKSKKPGWVFLTLLWGMSLLFFSTSPTDAQEPSEDLEAQIP
                     ELINQLKGKDESLRVEASNKLATIGKPAVPALIGVLTEKEAAPVAIFILGRIGAGAKE
                     AIPTLSKTLKQHEDKNVRARAAEALGKIGPETFLLIVPGLKDKEAVVRLRAVQALGRI
                     GSPAEPVLSSVVDCLQDADEGVRTEA"
BASE COUNT         1187 a         1215 c         1249 g         1173 t
ORIGIN      
        1 gggctgaaga ggcgcgcaag gctgaggaag ctcgccgcaa ggctgaggaa gaatcacgcc
       61 gggctgaaga agcacgcaaa gccgaagaag cacgccgcaa ggctgaggaa gctcgcaagg
      121 ctgacgaggc acgcaaagcc gaagaagctc gtcgcaaggc ggaagaagaa gctcgtcgta
      181 aagctgcgga agaagaagct cgtcgtaagg ctgcggaaga agaagcccgt cgcaaagctg
      241 aagaggatgc ccgccgccgg gctgacgaag cccgtcgagc cgaagaagct cgcaaagctg
      301 aagaagctcg tcgagccgaa gaagcccgcc gcgctgccga agccgccaag aaagctcagg
      361 aagctcagtt taagcctccc gtttttgagt taccgccaga agaaccaccc aagccaaaag
      421 cgccagagcc agagcaggtt ttctttgtac cgccgggtgg tgcggcgcca gagccaatcc
      481 agcctccaaa acctcagcca tcacccattg ccgcgcaggg taccattcct ttgacttcat
      541 ttaccccacc tgctgggtac cagtcagccc caccaccgcc atcaagcacg gcaccgggtg
      601 tttttaagta tgatccggcg aaatacaaag gctcagaagc cgatcataaa accgcccagc
      661 gtgtggctcg cacgattgtg agcgatatca agatctacaa tgaaaagaag gttgttgaag
      721 gccgcaaatc aaagaacctg tatgaccttc tcaagaaaga gtttgatcac ggtctcaaaa
      781 cctatgagga ccggattggc gaagttgtcc gcgcggaatc aacctatctg tatgacacga
      841 ttgttgacaa actatgtgat ggcgatccgt cgtgccttgg cccaaacttc ccgaagaatt
      901 attagtcctg attgactttt gttgatctca aaaggttcag actggaattt tccagcactg
      961 aacctttttt attttcgtca gccgaattga gccagccgtc agtgatcaga ttctgagccg
     1021 tttacaaatg aacgattcct gaaccgatag ccatacagca aaaaattggc aacgcattca
     1081 atgactgatc actgaccaaa actattggcg gagcaagaac ccatgaaaaa cagaacctgc
     1141 acctggtggt ggctctatgg acttggattg attgtgttga gtggctatca cagcatcagt
     1201 gccagagaaa caccgctttc agaccgggtt gtggtgtacc agattgaagc tgtacttgac
     1261 ccggtcgcca aacacattca gggaaaggaa acgcttcgct ggcgcaatcc atcagatgcc
     1321 ccggtgactg aactccaatt ccacctgtat ttaaatgctt ttcgtgatca gaaaagcacc
     1381 tttatggtcg aatcaggtgg ccagttacgc ggggattatt tgaaaagcgg gaagtttggc
     1441 agcattgatg tgctgtcact cagaaccagt gatggggaag acctgctgcc gcgagccgaa
     1501 tttattgccc cagatgatag caatcgcgat gaccggacag tctggcgaat tcccctgacc
     1561 agacccgttg gcccacatca ggaaatcacg ctctcaatcg agtttctgtc caaattgcct
     1621 cgggtttttg cccggactgg ctatctggga aactattttc tggtggcaca atggtttcca
     1681 aagcttggtg tctatgaacc ggctgggctt cgccgtcgga ccacatctgg ctggaactgt
     1741 catcaatttc atgccaacac ggagttctat gccgactttg gcacctatga tgtgaccttt
     1801 gtggtgccgg aaacctataa agtcggtgcc accggtgtac tccaatcaaa agaatctcgg
     1861 cccggaaatc aaatggcata tcggtatgtt caggcggatg tgcacgactt tgcctggacc
     1921 gcatcacctg attttcttga atttgaagac accttccgcc atcctgacct ccccgaagtc
     1981 aaactcaagc ttttgctcca gccttcacat ttagcccaga aagaccgcca tctggcagcc
     2041 gttaaaaaca gtctgcagta ttgtggcctg acctatggtg cctatcccta tcaaaccctc
     2101 acgattgttg atccggcata tcaggctgat ggctcgggtg gaatggaata cccgactctg
     2161 attacctgtg gcaccctcta tcgtgtgatg gatgacgaag ctttgtttga agcctcgcca
     2221 gaagtcgtca ccactcacga atttgcccat caatactggt atggcatggt ggccagcaac
     2281 gaatttgaag aatcgtggat ggacgaaggc tttacctctt ttaccgagcg caaattgatg
     2341 gatgcgtttt ataaagacca gactgcgata tggtttcggc tggttggaca acccattatg
     2401 agattgccca ttcggctgga tgtgtggtcc tttaatcgag tgactttgac tccagccact
     2461 gtgaaatgcg accccctgat gacccagagt tggaagtaca aaaccgatga tcaatgtgag
     2521 gtgagttact atttcaattc ttaccctcga ccagccctga cgcttgagat gctggaacgc
     2581 cacctgggaa caccaaccat gaaccggatc ttgaaaacct actttgaacg ctggcgcttt
     2641 aagcatccaa ccgcccagga tttctttgat gttgcctcag aagttgccgg acagaatctc
     2701 gactggtttt ttcagcagta ttttcgcgac acggtcgtgc ttgactatgc gattcaatca
     2761 gccaaaacca ccgggactgg gacccagttt gtcaccgagg ttaccgtggc tcgcaaaggc
     2821 gaagcggtga tgccagtgga ggttgaattc cgatttacag atggcagccg gttgcgtgaa
     2881 acgtgggatg ggaaagctgc cgaaaagcga tttgccgtcg agcggtcagc cgcacttcag
     2941 tctgtggtgg ttgaccctga ccacaaactg atgctcgacg ttgactggtt taacaacagc
     3001 tatgttgtga acccttctca ggtcggtacc gtgaaccttg tttctcgtct gatttttctt
     3061 ttacaggaag ctctatgttt cgcattgtaa ttaactcttt ggttcaggcg gcccgacact
     3121 ggcgattgat cctgatgctg atgctggcga cgtggtgtgc ctcaaccctg gtggcttttc
     3181 caatctacgg gttattgaac cggctcaatg gtaattcaga aatggcagac cgattgcttc
     3241 aaccagatct ggatgccgcc tggctggctg aagtcgctca ttttccggaa ctggtatccc
     3301 agtatggggc ctggttcatt ggtggactga tggttgttgg tttggggtat tggatcgttg
     3361 ggatttatct gactggtggg ttgattggac tgtttgttga tcccgaaaaa cagagttccg
     3421 ggatggaaaa ccagggttcg gggttccggg ttccgggttc cgggtcagat cctggaaatt
     3481 cacaccagac gatgagttca gaaccgctca ttcctggcga aatggtgaac acaaacaact
     3541 gggaatcgga acccggaacc cggaacccgg aacccggaac ccggaaccct gggtttctcg
     3601 ccaactgtgc ggcttccttt gggactttta tgcgtgttgc cttgatatca gtctttctcc
     3661 acagtctttt gatctgcctt ggttttttgg gatggcggct catttcacag tatgctgaga
     3721 cttcagccaa tgagccccag gtacgtttct tgcggatgat ggtgtatttg ctggcaatac
     3781 tgttcctggt cgtgattcac ctgtttttgg attatctcaa aattcgaatt gttctaactc
     3841 gccaggcctg ggtcacgcgt caaattcttc caacccttat cttgattgtg cgtcactggc
     3901 aaaaactgat cggagtgttt ggtttgatgc tggtgatcac cctggttgtc caggtcctgc
     3961 tcgctcaaat ctcattttcc atttcaccca acaactggtt cctggttggg ttgggcggtg
     4021 tgacgcacca ggttgtcata atgactcgct ggtggctcaa attggggtgg tacgccagtg
     4081 aagtcaacgc ggtacaaagc acaggtgcat aaatccagga aacttctttg aataattctg
     4141 agtcgagcat cgcaacccgt gaaaatttcc tctaaactac ctgatgtcct gccacgtcac
     4201 aacccaacac aattcaacca ggcacagtga gatagaagcg tttctcttcg gtgaaaagga
     4261 gggagcatgc gatgttcaaa gtcgaagaaa ccaggatggg tgtttttgac acttttgtgg
     4321 ggaatgagct tactgttttt ttctacgtca cccactgatg cccaggagcc atctgaggat
     4381 cttgaagccc aaattcccga attgatcaat caacttaaag gaaaagacga gtcacttcga
     4441 gttgaagcca gcaacaaact ggcaacgatt gggaaaccag ccgttccagc actcattggc
     4501 gtgctgactg aaaaagaagc cgccccagtt gccatcttta tcctcggacg aatcggcgcc
     4561 ggggccaaag aggcgattcc aaccctgtca aagacactca aacaacacga agataaaaac
     4621 gtccgggcgc gagctgctga agcgcttggg aaaatcggac cagaaacctt tctgctcatt
     4681 gttcccggct tgaaagacaa agaagcggtt gttcgtctgc gtgccgttca ggcgcttggg
     4741 cgtattggct cacccgccga accagtctta tcctcagttg ttgattgcct tcaggatgca
     4801 gacgaagggg ttcgcactga agct
//
LOCUS       DAOQDO010000134         4820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11593_length_4820_cov_3.377335, whole genome shotgun sequence.
ACCESSION   DAOQDO010000134 DAOQDO010000000
VERSION     DAOQDO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11593_length_4820_cov_3.377335"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1108
                     /locus_tag="PKZ53_03250"
     CDS             134..1108
                     /locus_tag="PKZ53_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ39480.1"
                     /translation="MSLLTVNHLQTYFYTRQGVVRAVDDVSFSLEQGQALGIVGESGS
                     GKSVTALSIMGLVGGTGKITGGEIHYNGTNLLNLAADDRRRMRGSEIAMIFQDPMTSL
                     NPVFTIGEQIAEAIRLHKNVPRKQAWDAAVESLQSVSIPDPAKRARDYPHQLSGGMRQ
                     RAMIAMALSCDPKILIADEPTTALDVTIQAQILELINQLRVERQLALLLITHDLGVVA
                     ETADRVAVMYAGRVVEDAAVRDLFANPQHPYTAGLLASIPRLGDRTRNRLNTIEGVVP
                     KLTELPPGCAFAPRCGEAQPQCRDGEIVLGRLKTATDHKVRCRMRITE"
     gene            complement(1306..1872)
                     /locus_tag="PKZ53_03255"
     CDS             complement(1306..1872)
                     /locus_tag="PKZ53_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39481.1"
                     /translation="MRFIVIGILMYALTAPVFGSTGPTPASSHVVAASWYEIISPSRR
                     WDNKSLARDEARRQRHDLYETFVKLRQTSEELSQLLDQNITYTERQKAAKKASKIATV
                     ARKTWNELQFHDPVYQHPGDKLSFSPRGLAVALKERDRVALQVRRTESTLASMQRMIV
                     DHNQYLSMALQQLEALEQIARQIETDVK"
     gene            complement(2161..2853)
                     /locus_tag="PKZ53_03260"
     CDS             complement(2161..2853)
                     /locus_tag="PKZ53_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020609750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNJ39482.1"
                     /translation="MIKPNLYQFLPLNFQKLPLETQLAEAKAFHDRLLTRRTVRDFAP
                     DPVPFELIESAIHIAAHAPSGANQQPWRFVVVQDADIKRQIRLAAEAEERENYGNRFP
                     DDWLQVLAPLGTDWHKEFLEIAPYLIVVFKIDYDLAPPETEGGQPRHIKHYYVNESVG
                     IAVGFLLAALHFSGLATLTHTPNPMKFLTEILGRPKNEKPYVLIPVGYPAEGARVPDI
                     TKKPLSDVMQVI"
     gene            3041..>4820
                     /locus_tag="PKZ53_03265"
     CDS             3041..>4820
                     /locus_tag="PKZ53_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ39483.1"
                     /translation="MKPHISVVTALLLLGSWSAPPVLALTARPHLETAQEKSESPASL
                     AKLPNGIERVTSLAGITEYKLANGLKVLLFPDQSKQTITVNMTYLVGSRHENYGETGM
                     AHLLEHMLFKGTPKHPNIPEELDKRGGRYNATTSYDRTNYFEILQATDESLRWALELE
                     ADRMVNSFIAKKDLDSEMTVVRNEFELGENSPFSILIERIQSTAYLWHNYGNSTIGAR
                     EDIEKVPIERLQGFYRTYYQPDNAVLLLTGKFDEAKVLTYINETMGLIPKPLRALQNT
                     YTLDPVQDGERAVTVRRTGDIQILAAAYHIPAGSHADFAALELLSTILGDTPSGRLHK
                     VLVDSGKATGVFGSNFQLREPGLAAFGAVVPKNQSLDEVRDLFLKALEQITAQPITAE
                     EVERARLQALKNVELTVNSSDQFGLELSEWIAMGDWRLFFLHRDRLKKVTIDDVQRVA
                     NTYLIASNRTLGTFIPTDKPVRAEIPASPDVAKLLEGFKSDDLVEAGEVFEPTPHNIE
                     SRVVRSETKGGIKLALLPKKTRGGIVIGNLRLHLGSEKTLTGRDLAGELVGQMLMRGT
                     KKHTREQLQDELDRLKARLGVYGGATA"
BASE COUNT         1231 a         1179 c         1260 g         1150 t
ORIGIN      
        1 gtgctctctg tggttcaaaa aagccagggc tgaaatcttc tggataaaat gataaggtaa
       61 gcagatcact caggaggcag gatgaatttc ccttcatccc acattccaca tccttcaaaa
      121 cttcactcca ttcatgagtc ttcttactgt aaatcatttg caaacctatt tttatacccg
      181 gcaaggcgtt gttcgggcag ttgatgacgt cagtttttcg cttgagcagg gacaggcgct
      241 tggaatcgtc ggggagtccg gttcgggaaa atcagtgacc gcactttcaa ttatgggact
      301 ggttgggggg acggggaaaa ttaccggcgg ggagattcac tataacggta caaacctcct
      361 gaaccttgct gctgatgatc gccgccgaat gcgtggttcg gaaatcgcca tgatttttca
      421 ggatccgatg acatcgctca atccggtgtt caccattggc gaacaaattg ccgaagccat
      481 ccgcctgcac aaaaatgtgc cgcgcaagca agcctgggat gcagcggtcg aatcattaca
      541 atcggtttcg attcccgacc cggccaaacg cgcccgtgat tatcctcatc aactttcggg
      601 tgggatgcgc caacgggcga tgattgccat ggcgctcagt tgtgacccaa aaatcctgat
      661 tgccgacgaa cccaccacgg ccctggatgt caccatccag gcccagattc tggaactcat
      721 caatcagctt cgggtcgagc ggcaactggc actcctgttg attactcacg atttgggagt
      781 tgtggcggaa accgctgacc gtgtggcggt gatgtatgcc gggagagtcg tcgaagatgc
      841 cgccgtccgc gatttatttg ccaacccgca acatccatac accgcaggat tactggccag
      901 tattccacgt ctgggggatc ggacacgcaa ccggctcaac accatcgaag gcgttgttcc
      961 aaagttgact gaacttccgc caggatgtgc ctttgcccca cggtgtggcg aagctcagcc
     1021 tcaatgccgt gacggggaaa ttgtcctggg aagactcaaa acggcaaccg accataaagt
     1081 ccgttgccgc atgcggatta ctgaatgaag aatgaagaat gaagaatgaa gaatgagcct
     1141 ggtacttagt acctggtgct tagtacctgg tacctgatac ttgggatcca atactttcga
     1201 agaaccaaga accaggaacc aagcaccaag aaccaagaac caggaaccaa gcaccaagaa
     1261 ccagttttct tcattcttca ttctcaattc ttcattcaag cggatttact tgacgtcagt
     1321 ttcaatctga cgggcgattt gttcgagggc ttcgagttgc tggagtgcca ttgagagata
     1381 ctggttatga tccacaatca tgcgttgcat gctggcgagt gtgctttcag tccggcggac
     1441 ctggagtgcc acccggtctc gttctttgag cgccacggcc agaccgcgtg gcgaaaacga
     1501 caacttatca cctgggtgct ggtacactgg atcgtgaaac tgcaattcat tccacgtttt
     1561 acgggcgact gtggcaattt tacttgcttt tttggctgct ttttggcgct cggtataggt
     1621 gatattctgg tcgagcagtt gagagagttc ttcggacgtt tgccggagtt tcacaaacgt
     1681 ttcatacaaa tcgtgcctct gacgtcgagc ttcatcgcgc gccagtgatt tgttgtccca
     1741 tcggcgggat ggggaaataa tttcatacca cgaagccgct accacgtgtg acgacgctgg
     1801 agttgggcca gtagatccaa aaacaggggc agtaagggcg tacatgagaa ttccaatgac
     1861 gataaatcgc atagaaaacc tcctcgaatc aatatcttga gtgagatatt catttcaaaa
     1921 gcagttgaat accagactca atccactttg attattttca gaatgatatg cgatgtgctg
     1981 cgaaccaatt tcacccaaaa aggcacacgc aatatagaga agttttgggg agttactgag
     2041 aatataggaa gaatgagaca atgtaccaga tcattacagc tttcttcaaa aaattcatgt
     2101 ttttgaagaa agctgttggt caaaacgggg aacccctgcc actacactcc agtcttcggg
     2161 ttaaataacc tgcatcacat cagaaagtgg ttttttggtg atgtcgggta cccgggcgcc
     2221 ttcggctggg tagccaaccg gaatcaagac atacggcttt tcatttttcg ggcgacccag
     2281 gatttcagtt aagaacttca tcgggttagg cgtatgggtt aaagtcgcca ggccagaaaa
     2341 gtgcagcgcc gccagcagaa acccaactgc aatgccaact gattcattga cataatagtg
     2401 cttgatatga cgtggttgac caccctcagt ttcaggcgga gcaaggtcgt agtcaatttt
     2461 aaagaccacg atcaaatacg gggcaatttc cagaaactct ttgtgccagt cagttccaag
     2521 cggcgccagg acctgcaacc agtcatccgg aaagcgattg ccgtagtttt cgcgctcttc
     2581 ggcttcggcg gccaatcgaa tctggcgttt aatgtcggca tcctgcacca cgacaaaccg
     2641 ccagggttgc tggttggcac ccgacggtgc gtgggcggct atgtgaatcg ctgattcgat
     2701 caattcaaat ggtaccgggt caggcgcaaa gtcacgcacg gtgcgtcggg tcaggagccg
     2761 gtcgtgaaag gctttggctt cggcgagctg agtttcaagc ggaagtttct gaaaattgag
     2821 cggcaagaat tgatagagat ttggtttgat catacggtga ggcttctttt catcggaaat
     2881 aaaatagagt caaccagccc agaattcaca ttcgtgaaaa actattcgtc aagtggctga
     2941 aaaaagggta aaactctccg cactcaaaag ccaaacagat tcgaagaaaa tttggagcgc
     3001 ggctcacctg acgtcgcttt ccagagacga gggaaattga atgaaaccgc atatttcagt
     3061 tgttacggca ctcttgttgc tggggtcgtg gtctgctccg ccagtcttgg ccctgacagc
     3121 ccggcctcat cttgagaccg cacaggaaaa gtctgaatca ccagcatcgc tggcgaaatt
     3181 gcctaatggc atcgagcggg tcacttcgct ggcgggaatt accgaatata aactggccaa
     3241 tggcctcaaa gtccttttat ttcctgatca atccaaacag acgatcacgg tgaatatgac
     3301 ctatctggtc ggttcccggc acgaaaacta cggcgagacc ggcatggcgc acctgctgga
     3361 gcacatgctg tttaaaggaa cgcccaaaca tccaaacatt cctgaagagc ttgataagcg
     3421 cggtggtcgt tacaatgcca ccacttcgta tgatcgaacc aactattttg aaattctgca
     3481 ggcaaccgat gaatcgttac gctgggcact cgaactcgaa gctgaccgga tggtcaatag
     3541 ttttattgcc aaaaaagacc tcgattcaga aatgacggtt gtgcgcaatg agtttgagtt
     3601 gggcgaaaac agcccgttca gtattttgat tgaacggatt cagtcaacgg cctatctctg
     3661 gcacaactat ggcaattcaa ccattggcgc tcgtgaagat attgaaaaag ttccgattga
     3721 gcggctgcaa ggtttttatc gaacctacta tcagccggac aacgccgttt tgttgctgac
     3781 tgggaaattt gatgaagcga aagtgttgac ctacatcaac gaaaccatgg gtctgattcc
     3841 aaagccactc cgggcactcc aaaacaccta caccctggat ccggtgcagg acggtgaacg
     3901 agcggttacc gtgcgccgca caggtgatat tcagattctg gcggctgcct atcacattcc
     3961 ggctggcagt cacgctgatt ttgcggcgct tgagttactt tcaaccattt tgggggatac
     4021 accgtcaggt cgcctgcaca aggttctggt cgatagcggg aaggcgacag gtgtttttgg
     4081 atctaatttt caattgcgcg agcctgggct ggctgctttt ggggctgtgg tacccaaaaa
     4141 ccagtcgctg gatgaagttc gtgatctgtt cctgaaggca cttgaacaga ttacggccca
     4201 gccgattaca gccgaagaag tcgagcgcgc ccggttgcaa gcgctcaaaa atgtcgaatt
     4261 gacagtgaat tcttccgacc agtttggtct tgaactcagc gagtggatcg cgatgggaga
     4321 ctggcggctc ttttttctcc atcgcgaccg gttgaaaaag gtgacgattg atgatgtcca
     4381 acgcgtcgcc aatacgtacc tgattgcgtc caatcgcacg ctcgggacat tcattccgac
     4441 tgacaaacca gtccgggctg aaattccagc ttcgccggat gtcgcaaaac ttctggaagg
     4501 attcaaaagc gatgatctgg ttgaagccgg tgaagtcttt gaaccaacac cccacaacat
     4561 tgaatcacgt gtggtgcgca gcgaaaccaa aggtgggatc aaactggcgt tgctgccaaa
     4621 gaaaacccgt ggcggtattg tgatcggtaa tttacgcctg cacctgggtt cagaaaaaac
     4681 actgactggt cgggatctgg cgggcgagct ggtcggccag atgttgatgc gcggcacgaa
     4741 aaaacacacc cgcgagcaac ttcaggacga acttgaccgg ttaaaagccc gattaggtgt
     4801 gtatggcggg gccacggcgg
//
LOCUS       DAOQDO010000135         4809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11641_length_4809_cov_2.748633, whole genome shotgun sequence.
ACCESSION   DAOQDO010000135 DAOQDO010000000
VERSION     DAOQDO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11641_length_4809_cov_2.748633"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKZ53_03270"
     CDS             <1..661
                     /locus_tag="PKZ53_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNJ39484.1"
                     /translation="NVNLRPFYGEGSKSVGYEIAEQLGWRAPDAVIVPMAGGSLITKI
                     HKGFKELELLGLLDGEVKTRLYGAQAAGCNPIVSATKQGTNIIRPVKPKTIAKSLAIG
                     NPADGYFAANVIQQSGGCGEDVTDQEIIDGIQLLAETEGIFTETAGGVTVAVARKLVA
                     TGKLNRNGVTVITITGNGLKTQEALPLTTPLVISPSLREFQDVLEQEFSTQPAYATAG
                     A"
     gene            816..1091
                     /locus_tag="PKZ53_03275"
     CDS             816..1091
                     /locus_tag="PKZ53_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015139615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNJ39485.1"
                     /translation="MAQSILIPTLLRQLTGGSEVIQLQAATIGEALTTLDSQFPGFRA
                     RVCDETGQLRRFINLYLNGEDIRFLDNLATPLPDTAEVAIIPAIAGG"
     gene            1153..2715
                     /locus_tag="PKZ53_03280"
     CDS             1153..2715
                     /locus_tag="PKZ53_03280"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNJ39486.1"
                     /translation="MRDEREPILQPPALSPTSSARRLQPEDFAARCLLEFNMHTHTSH
                     NLVVLKFGGTSVKDARAIRATAEIVASRRDCHPVVVVSALAGVTDGLLKLAHSAVDTP
                     IATTLKTLKRLEVRHLEVLGELTSGAPCLAARLFRQEYETLLESKFAELELLLCSVAT
                     LGACTPIHQDAIVAFGERLSSILVTAALQVVGIQAEVFDVRQVMITTDDFTEASPLVE
                     ELNRRVTARLLPLIAEGIVPVTQGFVGATLSGATTTLGRGGSDFTATLIGAALRAQSV
                     EIWTDVDGVMTADPKLVPEAVTIPELSYHQAEELALRGAKVLHPKTLRPVRDLEIPVW
                     IRNSSNPSTPGTLVCARPAHPPQTSLACQRNLVRLQVLLSGTNALTSSLSALATLFSK
                     LKLFPEHMVCTPTAITLLLPANQVTQALEAMLGELGSLQIERHQALVAVIGQMVTAQP
                     VKGVEFSEAEWIQPDLSNGRFLIATSERFLGRVVRCLHDQFVQLPMNQVLTTSNLLCQ
                     SDCRELTVAAVA"
     gene            complement(3111..>4809)
                     /locus_tag="PKZ53_03285"
     CDS             complement(3111..>4809)
                     /locus_tag="PKZ53_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ39487.1"
                     /translation="KVDENAGTIHLNNRRVLIIDADAFGLLRKELIETLGMIEARRML
                     SRFGYMRGYRDALTSKDLFHGQDPQLWSQSGVQFHSQEGIVRPELEKFTFDPATGVFE
                     VIGQWKNSFEAEQHIKHLGKSETPTCWTLMGYASGFQSVVMGREVFFLETSCVGRGDK
                     ACRFIGTVDESPEGPIATLFPKIYQVEDMPEAMLRVRQQRTSLKSAIAELEQRSKELA
                     DEKQKVQALEKQVMCLQESLNEPYNFEEMIGANHAFRAIVAEAELVAQSDSTVLITGE
                     TGTGKELLARAVHARSLRASRPLITVNCAALPAGLVESELFGHEKGAFTGAVQRKPGR
                     FELANKGTIFLDEIGELPPETQSKFLRVLQEGTFERVGSSQTIQVDVRVIAATNQPLD
                     HLVEAGKFRPDLFYRLNVFPLHIPPLRERENDIVLLTNFFIQKYRSRLKKKITAISQE
                     SIEQLKRYSWPGNIRELEHIIERAVLVSDSPILTIDPSFSVKQRPVSPSISPTPAPPL
                     KLQTLEDHEREYISQILKHTNGMIGGKHGAAEILGVPSTTLRSRMQKLGMLPGRKNA"
BASE COUNT         1104 a         1239 c         1313 g         1153 t
ORIGIN      
        1 caacgtcaat ttgcgtccat tttatggcga aggttcaaag tcggtcgggt atgaaattgc
       61 cgagcaactc ggctggcgcg cgcccgatgc ggtgattgtt ccaatggcgg gtgggtcgct
      121 cattaccaag attcataaag ggtttaagga actggaactg cttgggttac ttgatggaga
      181 agttaaaacc cgtttgtatg gtgcccaggc cgccgggtgt aacccaattg taagcgccac
      241 gaaacagggc acaaatatta tccgcccggt gaaaccaaaa accatcgcca aatcgctcgc
      301 aattggaaat ccggcagatg gctattttgc cgcaaatgtg atccagcaaa gcggtggatg
      361 cggggaagat gtcaccgatc aggaaatcat tgacggcatt cagctactgg ctgaaaccga
      421 agggattttc accgaaacag caggcggcgt aacggttgcc gtcgctcgaa aactggtggc
      481 cacgggcaag ctcaatcgaa acggagttac ggttatcacg atcactggaa atggtctcaa
      541 aacccaggaa gcgcttccgt tgaccacgcc gctggtgatc tctccgtcac tgcgcgagtt
      601 tcaagacgtg ctcgaacagg aattttcaac tcaaccggct tatgcaaccg ctggcgcatg
      661 aactggagct gaaaacatgg gctcagggct tggggctgaa ggctcgcaag ctcggggcgg
      721 aagaaaacgg gtttcatacc ctgagcccgt tgtcttcagc cctaagcccg tagcccgttg
      781 ttttctgccc tgtatttcag aaaggacacg acatcatggc tcaatcaatt ttaattccaa
      841 cgttattgcg acaactcacg ggcggttcag aagttattca gctccaggct gcaaccattg
      901 gcgaggcgct taccacgctc gactcccagt ttccagggtt tcgcgcccga gtgtgtgatg
      961 aaactggcca gttgcgccgc tttatcaatc tgtacctgaa cggcgaagac attcgctttc
     1021 tggataactt agccacaccg cttccagata cggctgaggt cgcgattatt ccggcgattg
     1081 ctggaggata aaaacagggc tgaaaaaaca gggctcaggg ctgagagcga ttgaagggat
     1141 gagggatgaa ggatgaggga tgaaagagaa ccaattcttc aacctccagc cctgagcccg
     1201 acgtcttcag cccgacgtct tcagcccgag gattttgcag cccgatgtct tttggaattc
     1261 aatatgcaca cacatacatc acacaatctg gttgttttga aatttggtgg gacttcggtc
     1321 aaagatgccc gtgcgattcg ggccacggct gaaattgtag ccagtcggcg tgactgccac
     1381 ccggttgtgg tggtttcagc cctggccgga gtgactgatg gattgttaaa gctggctcac
     1441 tcagccgtag atacccccat cgctacaact ctaaaaacat tgaaacgact ggaagtacgg
     1501 catttagagg tgctgggcga gttaaccagt ggtgcccctt gcctggcagc ccgattgttt
     1561 cggcaggaat atgaaacact ccttgaatcc aaatttgccg aactggagtt gctcctgtgc
     1621 tcggtggcaa ccttgggcgc ctgcacgcca attcatcagg atgccattgt ggcttttggt
     1681 gagcgactta gttctatcct ggtgacggcg gcgcttcagg ttgtcgggat tcaggccgaa
     1741 gtttttgatg tgcgacaggt gatgatcaca accgatgact ttaccgaagc ttcaccactc
     1801 gttgaggaac tcaaccggcg ggtgacagcc cgattgctcc cgctcattgc tgaaggcatt
     1861 gttcctgtca ctcaaggttt tgtcggcgcc acgctttccg gcgccaccac aacacttggc
     1921 cgtggtgggt cagatttcac cgccacattg atcggagcgg cactccgggc ccaatcggtc
     1981 gaaatctgga ccgatgtaga cggcgtgatg acggcggatc caaagctggt gccagaggcg
     2041 gtgacgattc ccgaactttc gtatcaccag gctgaagaac tggcgctccg aggggccaaa
     2101 gtattgcacc caaaaacgtt gcgcccggtt cgcgaccttg agattccggt ttggattcga
     2161 aattcatcga acccttcaac gcctggaaca ctggtgtgcg ccagaccagc ccatccgcca
     2221 cagacatcac ttgcctgcca gcgcaatctg gtgcggttgc aggttttact gtcaggcacc
     2281 aatgcactaa cttccagtct ttctgccctg gcaacgctct tttcgaaatt aaagctcttc
     2341 ccagaacaca tggtgtgcac tccgaccgcc attaccttgc tgttgcccgc caatcaggtg
     2401 acgcaggcgc tcgaagcaat gcttggtgaa ttggggtcac tccagattga gcgtcatcaa
     2461 gctctggtgg ctgtgattgg tcaaatggtg actgcccaac ctgtcaaagg agttgaattc
     2521 tctgaagctg agtggattca accggatctt tcgaatgggc gattcttgat tgcgacgtcg
     2581 gaacggtttc tcggacgggt ggttcgctgc cttcacgacc agtttgttca gttgccaatg
     2641 aatcaggtgt tgaccacctc aaatttgctg tgtcagtcgg actgtcgcga gttaacggtt
     2701 gccgccgttg cctgagtctg atttgacacc aaccacattc accgcacagt acgtcgctgg
     2761 cgcaatcgct caacttgcgg agtcgagaga gggatgggat acccagattg cgtccccggc
     2821 gtcctgtgcg gtgattattt ttgggctgaa gactttgggc tgaagaagac gggctgaaga
     2881 agacgggctg aagaagacgg gctgaagaag ttgggctgaa gaaaatgggc tgaagaaaat
     2941 gggctgaaga agttgggctg aagaaagcgg tttcagggta tgaaacccgt tttcttcagc
     3001 cccaagccct gagcccaagg attgttcagc cccgagcctg cgagtcttca gccccaagcc
     3061 ctgagcccaa ggattgttca gccccaagcc caaagttttc caccccagga tcaggcattt
     3121 ttcctgccgg gcaacatccc gagcttttgc attcggctgc gcagggtggt cgaaggcacg
     3181 cctaaaattt cagccgcccc gtgcttgccg ccaatcatac cgttggtgtg tttcagaatt
     3241 tgggaaatgt attcgcgttc gtgatcttcg agggtttgca atttcagtgg tggcgccgga
     3301 gtcggagaga ttgagggtga gactggccgc tgcttaaccg aaaaggaagg gtcaatggtc
     3361 aaaattggtg aatctgaaac cagcacggcc cgttcgataa tgtgttcgag ttcacgaata
     3421 tttccaggcc acgagtaccg ctttaactgc tcgattgatt cctggctgat ggcggtgatt
     3481 tttttcttga gccgggagcg gtatttctga ataaaaaagt tggtgagcaa cacaatgtcg
     3541 ttttcacgct cacgcaaagg cgggatatgc agtggaaaga cattgagtcg gtaaaacaga
     3601 tcaggccgaa actttccagc ttcaaccagg tgatccagcg gctggttggt ggcggcaatc
     3661 acccgaacat caacctgaat ggtttgtgaa ctgcctacgc gttcaaacgt gccttcctgg
     3721 agtacccgga gaaactttga ctgagtttcc gggggaagct cgccgatttc atccagaaaa
     3781 atcgtgcctt tatttgccag ttcaaaccgg ccaggtttgc gttgaaccgc cccggtgaag
     3841 gcgccttttt catggccaaa tagctcactt tcaaccaggc cagccggcag ggcagcacaa
     3901 ttaacagtaa tcaatggcct tgaggcgcgc aggctgcggg cgtgaacggc acgggccagg
     3961 agttcttttc cggtcccggt ttcgcccgtg atcaaaaccg ttgaatctga ttgggcgacc
     4021 agttcggctt cggccacaat agctcgaaag gcgtggttgg caccgatcat ttcttcaaaa
     4081 ttgtagggtt cgttgaggct ttcctgcagg cacatcacct gcttttccag ggcctggact
     4141 ttttgcttct catccgccag ttcttttgag cgttgttcga gttcagcaat cgctgatttc
     4201 aggctggttc gttgttggcg cacccgaagc atggcctccg gcatgtcttc aacctgataa
     4261 atttttggaa agagtgtggc aatcggcccc tctgggcttt catcaacggt tccaataaac
     4321 cggcatgctt tgtcgccccg tccaacacaa gacgtttcca gaaaaaacac ttcgcggccc
     4381 atgacgacag attgaaaccc gctggcatag cccatcagtg tccagcaggt cggcgtttca
     4441 cttttgccaa ggtgtttgat gtgttgttca gcctcaaacg agtttttcca ctggccgatg
     4501 acttcaaaga cacctgtggc cgggtcaaag gtaaactttt cgagttcggg gcgaacaatc
     4561 ccttcctgag agtgaaattg gacgccgctt tggctccaga gttgtggatc ctggccgtga
     4621 aacaggtctt ttgaagtcag ggcatcgcga tatccacgca tatacccaaa ccgggaaagc
     4681 atccgacggg cttcgatcat tccaagggtt tcgatcaact ctttgcgcag cagcccaaat
     4741 gcatcggcat caatgatgag cacgcgccgg ttattcaaat gaatcgttcc agcattttca
     4801 tcaactttg
//
LOCUS       DAOQDO010000136         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11663_length_4802_cov_3.475458, whole genome shotgun sequence.
ACCESSION   DAOQDO010000136 DAOQDO010000000
VERSION     DAOQDO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11663_length_4802_cov_3.475458"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKZ53_03290"
     CDS             <1..415
                     /locus_tag="PKZ53_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ39488.1"
                     /translation="KAPGLAAMMLIFLLSLAGIPMTGGFIGKFYLFGSLLQSNIKWMN
                     ILAVIAVLNTVVSLYYYARFIRAMFLNPASEQVQVQLTPILRWTLTTAAVLVIVIGVY
                     PSPFIRFSKIAAQTMYVRYERPSKEPLWKPKFLQF"
     gene            complement(448..918)
                     /locus_tag="PKZ53_03295"
     CDS             complement(448..918)
                     /locus_tag="PKZ53_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNJ39489.1"
                     /translation="MLITTVQGNLLDQSVEVIVNTWNRNIIPWWLLLPQGVSGAIKRR
                     GGYQPFRELATFGPIPLGQAVLTSAGKLPFRGIIHVAGINLLWRASEYSIRQSVKNAM
                     QIVDREGFMSVAFPIIGAGSGGFKAGKALELMKDELMQCPSTATVRIVEFRAHS"
     gene            1895..>4802
                     /locus_tag="PKZ53_03300"
     CDS             1895..>4802
                     /locus_tag="PKZ53_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39490.1"
                     /translation="MSSNVPNPPESSPTTPNDSGDLRPEEQTLVDIEKELNERLVALG
                     LPLLTGGSAPAGRQTQVLTKSTGDGGGTTQVLDLRELRTALNQQIRIAEIKEQVIEVI
                     NDWLACMARTDGLVDPFEFELRAAALLIPYCREHIPDFPTSLHPAELAMDLNFQPMLR
                     EELGRKNQTGPLTEEQIEGCAVQIIQSLLNHPLLPQKEADQIEISLQTTTLRDKNGTS
                     VYTPSDGKFLKFLIEERQLLNRDGEIGLLAMLISGRKWNKIESEPLFVAAIEYTLEAL
                     ARKRGIDQIIIRSQGPDHPVAYILREFILHNISLSLEEQMSFVEGFIGMTKNILDRPP
                     EEFVEGIRREYVPRMKKIFHDLIHDSIHRFLQTFEREFLESKRSMRSKLFLLKRVGDC
                     CELTFYCYEAVRSMESPSGEVYARERMCEGTEHFARRNNIPVSMGMDFFNRKNTLAEL
                     IVDALKYRREQQLRSHTGGLRSPDPEVVRTKWRQLSSVLMSDLLFCFDQMRRTFDHDY
                     ARGRESGYENYQSWKEMVVRWCDSYARFLFGRSLSDVDRYRIESLASQQIEEDLAFVN
                     MHLPSVFRQGGQELGFEGAIRTRQPEDIIKENFLTFIKNFHSKLIGSDQQQLEQQAKF
                     FETYRGRTGPTLTQEDLQKRVFLARSDLPLSTYTLEVLTSFLAPETHDRAVLLQALET
                     GLELAQKAYIAEETRAVRVFLKQLQQRIGLLVILTEAERANFDERLTLTIKELLGREP
                     IGLASYILALKEIEDLRLSDERILEEWKNVETSIQRLQQIKVVLILTRRRAEIRDQVE
                     AFSNWLSVTPISQESPRRRTFDTRLQEIRQLVTQNQIQQSLLRLVLRIVRYVQIIASV
                     QNWNPPAAYILEDMLTLETRPRRIRELEAILAVYPVDQLDELVHKFHTTLWERFQESS
                     QSVAYYAPEEREVLTRRLERLQQLQARSDWQVTRARIGQSILSRLVKPFQKVLVA"
BASE COUNT         1225 a         1263 c         1170 g         1144 t
ORIGIN      
        1 caaagcccca ggtctggcgg cgatgatgct gatttttctg ctcagcctgg cggggattcc
       61 aatgacaggc gggtttattg ggaaattcta tctctttggc tcgctacttc agagcaacat
      121 caagtggatg aatattttag cagtaattgc cgtcctcaat acggtggtgt cgctttatta
      181 ctatgcccga tttatccgcg ccatgttcct taatccagcg tcggaacagg ttcaggtgca
      241 actgacaccg atcctgcgat ggacactcac cactgccgca gtcctggtca tcgtgattgg
      301 ggtatatcca tcgccgttta tccgattctc aaaaattgcg gctcaaacta tgtatgttcg
      361 gtatgaacgg ccttcgaagg agccgctttg gaaacctaag ttcctgcaat tttaatcaac
      421 ctcataattt aaaagctggc aactggttca agaatgggct cgaaactcta caatccgcac
      481 ggttgccgtt gaggggcact gcatcaactc gtctttcatc aattccagcg cttttccagc
      541 cttaaatcca ccagagcctg cgccaataat cggaaatgcc actgacataa agccttcacg
      601 atcaacaatc tgcatggcat tttttaccga ttgacggatg gaatactccg aagcccgcca
      661 cagcagattg atccccgcca cgtgaataat tccacgaaac ggcaacttcc ctgccgaagt
      721 caacaccgcc tgcccaagtg gaattggccc aaaggtggcc agttcccgga acggctgata
      781 cccaccgcgc cgcttgatag caccagacac cccctgaggt aaaagcaacc accacggaat
      841 gatgttccga ttccaggtgt tgacaatcac ctcaaccgac tggtcgagta aatttccctg
      901 aacagtggtg atgagcatcg tgaaaaacct cgggcctggg gcttggggct gaaaatacca
      961 gggctgaaga cttcgggctt cgggctgaaa aacattgggc tgaagacttc gtgtttcggg
     1021 ttacaatacc caatatcttc cgcccgtttt cttccgcccg aagtcttcca cccgatgtct
     1081 tccgcccgaa gtcttccgcc cgaagtcttc agccctggaa cttcccctac agtttgccca
     1141 cgttacctga gccgtaatag tctgaactta ccagcattcg taaggaattt tgctggactg
     1201 aaaggctgac aaaccgggat ttgcacactg gacagaacag tgagtcacca gcctggggct
     1261 ggacaaccgt tgaggaaatc ggttgaaaaa actttgcttt catctcaatt tttggttccg
     1321 gaccggtgta ttgataaatc tctgaattgc agaaggtgca gacgactgtt ttcataagaa
     1381 ttgagaatgc agaattgaga attgagaatt gagaatgcag aattgagaat gaagaaagtg
     1441 gtgagcggtt catggtgaga aatcaatact ttcaaaaaac caagcaccaa gaactaagca
     1501 ctaaaaggat tcttcattct ggattcccta ttctaccttc cgctgtactt cacatgccac
     1561 ctgtcggcaa aaatacagga caaattcttg gcaaatgaag ggcttccaac atgggcgagc
     1621 aacgactttc tatgctatgc tagccggaac ttacaggttt tccttctgat caatcgatca
     1681 atggccgttt tttcagtcaa tcatccattc aagcggtccc caataactct tgttgataga
     1741 acataacctt gatttcgaaa gccgagttct gcacgcactc tttatttcct cctggtcatt
     1801 ttgtttcatc aacctgttga acggacggtt tttcaaccaa atcgtctctc accatggttg
     1861 ggtaaacacg ttcaacgttt tttgaggcat tgcgatgagt tccaatgtac cgaatccgcc
     1921 tgagtcatct ccgacgacac cgaatgattc cggtgacctg cgcccggaag agcagactct
     1981 ggttgatatt gaaaaagaac tcaacgagcg gctggttgca cttgggttac cacttttaac
     2041 cggcggaagc gcaccggctg gccgccaaac ccaggtcctg accaaaagta ccggtgatgg
     2101 tggtggaacc acccaggttc ttgatttacg tgaacttcgc acggcactta accagcagat
     2161 tcggatagcg gaaattaagg agcaagtcat tgaagtcatc aatgactggc tggcctgtat
     2221 ggcccgcacc gatgggctgg tggatccgtt tgaatttgaa ctcagggccg cggcgctgct
     2281 gattccatat tgccgcgagc acatccccga cttccctacc tcactacatc cggctgaact
     2341 ggcgatggac ctcaatttcc agccgatgct gcgtgaagaa ctgggacgaa aaaatcaaac
     2401 ggggccactg actgaagaac aaatcgaggg ttgcgcggtc cagattattc aatccctcct
     2461 caaccatccg ctcctgcccc agaaagaagc tgatcagatt gaaatcagcc ttcagacgac
     2521 cactcttcgc gacaaaaatg gcacgtcggt ctatacccct tccgacggga agtttctcaa
     2581 gttcctgatt gaagaacggc aactcctcaa ccgggacggg gaaattgggc tcctggcgat
     2641 gctcatcagt ggtcgcaaat ggaataaaat cgaaagtgaa cctctgtttg tcgccgcgat
     2701 tgaatacaca ctcgaagccc tggcacgcaa acggggcatt gatcagatca tcattcgcag
     2761 ccaggggccg gaccatccag tggcctacat tttgcgcgaa tttattctcc acaatatttc
     2821 tctctcactc gaagaacaga tgtcgtttgt cgagggattt attgggatga ccaaaaatat
     2881 tcttgaccgg ccaccagagg aatttgtcga agggattcgt cgcgaatatg tgccgcgcat
     2941 gaaaaagatc ttccacgatc tgattcacga ctcgatccat cggtttttgc agacttttga
     3001 gcgagagttt cttgaatcaa aacgctcaat gcgaagtaaa ctctttcttc tcaaacgagt
     3061 tggcgattgt tgtgaattaa cattttattg ctatgaagcc gttcggagca tggagtcgcc
     3121 cagcggagag gtttatgccc gagaacggat gtgtgagggg acagaacatt ttgcccgccg
     3181 aaacaacatc ccggtttcaa tgggcatgga tttctttaac cggaaaaata ccctggcaga
     3241 actgatcgtt gacgccctca aatatcgtcg cgagcaacag ttgcggtcgc ataccggcgg
     3301 cctgcggtca ccggaccccg aagtcgtgcg gacaaaatgg cgacagcttt ccagcgtgtt
     3361 gatgagtgac ctgctgtttt gctttgacca gatgcggcgt acctttgacc atgactatgc
     3421 ccgtggacgg gaaagtggct atgaaaacta tcaatcctgg aaggaaatgg tggtccgctg
     3481 gtgcgatagc tacgcccggt ttctgtttgg gcggtcattg agcgatgttg accgttaccg
     3541 gattgaaagc ctcgccagtc agcaaatcga agaagacctg gcttttgtca acatgcacct
     3601 gccatcggtg tttcgtcagg gaggtcagga actcggattt gaaggcgcca tccgaacccg
     3661 gcagccggaa gacatcatta aagaaaattt cctgaccttt attaagaact ttcactccaa
     3721 gttgattggg agcgaccaac aacaactcga acaacaggcc aagttttttg aaacctaccg
     3781 ggggcggact ggccccacac taacccagga agacctgcaa aaacgtgttt ttctggcccg
     3841 gagcgacctg cctctgagca cctatacact ggaagttttg acttcctttc tggcgccgga
     3901 aacccatgat cgggccgttt tattgcaggc actggaaacc ggcctcgaac tggcccaaaa
     3961 agcctatatc gccgaagaaa cccgtgcggt tcgggtgttt ctcaaacaac tccagcaacg
     4021 gattgggctg ctggtaatct taaccgaagc cgagcgcgcc aattttgatg agcgactgac
     4081 gctgacaatc aaagaattgc ttgggcgcga accaatcggc ctcgcttcct atattctggc
     4141 cttgaaggaa atcgaagacc tgcgtctgtc agatgaacgc attttggagg aatggaaaaa
     4201 cgtcgaaacc agtatccagc gcctccagca aatcaaagtc gtgctgattt tgacccggcg
     4261 gcgagcggaa atcagggacc aggtcgaagc cttttcaaac tggttgagcg tgactcccat
     4321 ttctcaggaa tcgccccgcc gccggacctt tgataccaga ttgcaagaaa tccgccagct
     4381 tgtgacccaa aaccagatcc agcaatcgct gttgcggctg gtcctgcgca ttgtgcgcta
     4441 tgtgcaaatc attgccagtg ttcagaattg gaacccgcct gcggcatata ttcttgaaga
     4501 tatgctaacg ctggaaaccc gcccgcgtcg aatccgcgaa ctggaagcca tcctcgcggt
     4561 gtacccggtt gaccagcttg atgaactggt ccataagttt cacaccacgc tctgggagcg
     4621 gtttcaggaa agcagtcaat ccgtcgccta ctatgcccca gaagaacggg aagtcctgac
     4681 ccgccgtctg gaacgactcc agcaattaca ggcccgaagc gactggcagg tcacacgcgc
     4741 ccgaatcggc cagagcattt tgagccgcct ggtcaaacca ttccaaaaag tcctggtcgc
     4801 tg
//
LOCUS       DAOQDO010000137         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11666_length_4802_cov_2.474194, whole genome shotgun sequence.
ACCESSION   DAOQDO010000137 DAOQDO010000000
VERSION     DAOQDO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11666_length_4802_cov_2.474194"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PKZ53_03305"
     CDS             complement(<1..1327)
                     /locus_tag="PKZ53_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNJ39491.1"
                     /translation="MPVFTKRRCSRLLSTCLFLAVCLTSSLLLAMSFAPATASLGLNG
                     APGAQINRQPVMYRVSVANTGDVQRLIQLGLDVLEARGQGYVLVLGDQATPNQLAAHG
                     FRAEVDPELTRRSGFHPQTFFQGYRTVQEHYAHLDALAAAHPDLVAVVDYGDSWRKLN
                     GRPDGHDLKAVCITKLGPGDPVLSPTAGKPRFFLMAAIHARELTTSELAWRWMDYLVE
                     NYGVDADVTMLLDHTELWVVPVVNPDGRAIVEEGVEFPYFQRKNANDSLGNCDVPPSG
                     GNQAGVDLNRNAGFQFGGAGTSSDPCSLVYKGVGPSSEPEQYFLEALLLQLFADRRGP
                     QLTDAAPGDTSGVFITLHSFSDLVLLPWGWNECNGQPCAGRFRAPNDEGLRTLAFRLS
                     SFNEYTTGQGSEILYAASGSTDDWTYGTLGIASFTFEVGPESGACAGFAP"
     gene            complement(1381..1722)
                     /locus_tag="PKZ53_03310"
     CDS             complement(1381..1722)
                     /locus_tag="PKZ53_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39492.1"
                     /translation="MSKSRIEVFSALLEKQAADPMIWYGLANEYFKMSDWEKAAEALR
                     KVVEFNPSYTAAYQMLGSALANLGKIPEARQMWERGVSVATETGAWKARQHMEGLLAQ
                     HQTDSTSPFCS"
     gene            2083..3129
                     /gene="hemH"
                     /locus_tag="PKZ53_03315"
     CDS             2083..3129
                     /gene="hemH"
                     /locus_tag="PKZ53_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNJ39493.1"
                     /translation="MASPKISQPARIGVLLINLGGPDTLDDVKPFLYNLFSDPEIIQL
                     PIPALQKPVAWLISTTRHKKSANYYRQIGGGSPQRKITEQQAQALETELTQRGIQAKT
                     YVGMACWHPLVEDTFEQIKKDQITHLIILPLFPQFSVTTTGSAAKKLIRIINSQGGVR
                     HIRRSYITRYETHPAYIEALAQMTVAEFRKLPDQNPAATTLLFSAHSIPEKYVRRGDP
                     YLKQTQATVAAVMARVRYHLGAEPLHFLSFQSKIGPVKWLEPSTEVTLRKFAEERRKQ
                     VLAIPISFVSEHIETLYEMDILYKEMAEKIQLPFFRVPTLNCHPLFIQSLADLVETRL
                     RPQPQLVVKQAGQR"
     gene            3441..>4802
                     /gene="hemG"
                     /locus_tag="PKZ53_03320"
     CDS             3441..>4802
                     /gene="hemG"
                     /locus_tag="PKZ53_03320"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNJ39494.1"
                     /translation="MQDVIVIGGGISGLVCAYQLKRLGRNVLLIDGSNRVGGVMKSES
                     IDGFLFERGPNSFYITPEISELLDSIGISPYLVTADSSAPRFVYYKGKLRAVPVPTSP
                     LKFLSSFLTTRLLSPLAKLRLFAEPFIAPSTSGQEQTIEEFITRRFGKQFHDVLVSSF
                     VSGVYAGDTSELSAMASPPFSKLADLEAEHGSVIRGAFRSKRTAAAKAGEPARRAHQS
                     CSFLNGLETLPKALHKALVNELLLNCKIHSIEFHNHAPQAHYTLEVEFGGDRQPIHTA
                     SLIIATPAYTAARLLAPLSDKLANTLDQIPYPPLASVALALKESQLKLQPGTSLQGFG
                     FLIPRSEGVRTLGCIWNSSLFPERAPQGYVTLTCFIGGSTDPEVLNLNDDDIIRTVHK
                     DLQQTLGVSGDPRVLGVTRWKRAIPQYTIGHPSRRTEIEEQVGRYPGLYLTGNYMYGV
                     SIGD"
BASE COUNT         1192 a         1300 c         1146 g         1164 t
ORIGIN      
        1 ctggcgcaaa cccggcacag gctccagatt caggcccgac ttcaaaggta aaactggcaa
       61 taccaagcgt gccataggtc caatcatcgg ttgaaccact ggccgcatag agaatttctg
      121 acccctgacc agtggtgtat tcattaaatg aactcaatcg gaacgccagg gttcgcaagc
      181 cttcgtcatt tggtgcccgg aaacgacccg cacagggttg accattgcac tcattccatc
      241 cccagggcag caataccagg tcgctaaagc tgtggagggt aataaaaaca cccgaggtgt
      301 cacccggtgc agcatctgtg agttgcgggc cgcgtcggtc tgcaaaaagt tgcagcaaaa
      361 gggcttcaag aaaatattgt tcgggctcag atgatggccc tacgcctttg tataccaggc
      421 tacaggggtc actgctggta ccggccccac caaattggaa gccggcattg cggttgagat
      481 ccacgccggc ttgatttccc cctgacggag gcacatcaca atttccaagg ctatcgttgg
      541 cgttctttcg ctgaaagtag ggaaactcaa cgccttcctc cacaatcgcc cgaccatccg
      601 ggttgaccac cggtaccacc cacaattcag tgtggtcgag caacatggtt acgtccgcat
      661 caactccata attttcaacc agatagtcca tccatcgcca ggccagttcc gacgtcgtca
      721 actctcgtgc atgaattgcc gccatgagaa aaaaccgggg tttcccggca gttgggctca
      781 aaacagggtc acccggtcct aatttggtga tacatacagc ttttaaatca tgtccatctg
      841 ggcggccatt gagtttgcgc cacgaatcac cataatccac cactgccacc aggtccggat
      901 gggcagcggc cagggcatcg agatgggcat agtgttcctg aacggtgcga tatccctgaa
      961 aaaatgtctg cggatgaaaa ccggatcgtc gggttaattc cgggtctacc tcagctcgaa
     1021 atccgtgggc agcgagttga ttgggtgtcg cctgatcccc gagaaccaga acatagccct
     1081 ggcctctggc ttcaaggaca tccaggccca gttgaatcag ccgctgaacg tccccggtat
     1141 tcgcgactga tacccggtac atcactggct ggcggttgat ttgagcacca ggggcaccat
     1201 tcaatcccaa cgaagccgtt gccggagcaa acgacatggc aagcaaaagg ctgctggtca
     1261 ggcagacagc cagaaacagg caggttgaaa gcagtcgcga acaacgacgc tttgtgaaaa
     1321 caggcattcg atcacctcag gttaaacaat atggagtgcg acggtttgat acaacgttga
     1381 tcaagagcaa aaaggagacg tggaatccgt ttgatgctgg gcgagcagac cctccatatg
     1441 ctggcgagcc ttccaggcgc cagtttcagt cgcaactgac accccacgtt cccacatctg
     1501 gcgggcttcc gggatcttgc ccagattggc cagggccgaa ccaagcattt gataggctgc
     1561 ggtgtatgag ggattgaact ccaccacttt gcgcagagct tcagccgctt tttcccaatc
     1621 gctcattttg aaatattcat tggctaaccc ataccagatc atggggtcag cggcttgttt
     1681 ttcaagcaac gcggaaaaaa cttcgattcg tgatttagac atgccagtag ctccagacgt
     1741 gaatggggaa aatttaaatc tcctggggat tttttcacag ttcgacgttt gttcgcaaca
     1801 aaccggagcc ggacagaatc gtggaatttt gggtggcaag ggtggactcg gcgtgcgtat
     1861 acttgagcct gattcaagtg gcccaccagc acgcatccgt tgatacatct gtctttaagt
     1921 ttttgattca acagcatcta tccaaaccaa cccttcaggc tccctggttg gtgatccctt
     1981 tttgcagcct ccaactttca gtactcagac agcctcctga atctcaacct gtcccccgct
     2041 ggtcgtctag agacccaact ctttctgatc gaaacctgac ttatggcatc tccaaaaatt
     2101 tctcaaccag cccgaattgg ggttttgtta atcaaccttg gtggtccaga cacattggat
     2161 gacgttaaac cctttttata caatctgttc tctgaccctg agattattca gctaccgatt
     2221 ccagcgttgc aaaaaccagt cgcctggctt atttcaacaa cccgacacaa gaaatcagcg
     2281 aactattatc gtcagatcgg gggtggatca cctcaacgta agatcaccga gcaacaggcg
     2341 caggcgcttg agacagaact cactcaacgc ggaatccagg ccaaaaccta tgttggcatg
     2401 gcctgctggc atccactggt ggaagacaca tttgaacaaa tcaaaaagga tcaaattact
     2461 catttgatta ttcttccgct ctttccacag ttctcggtca ccaccaccgg ttcggcggcg
     2521 aaaaaattga ttcggatcat caattcgcag ggcggggttc gtcacattcg ccggagctac
     2581 attacccgct atgaaaccca tccggcttac attgaagcac tggctcagat gacggttgct
     2641 gaattccgga aactccccga tcaaaatcct gccgccacaa cactcctgtt tagcgcgcac
     2701 agcattcctg aaaaatatgt tcgccggggt gacccatatt taaagcaaac ccaggcgact
     2761 gtggcagcgg tgatggctcg ggttcgttat catttaggag ctgaaccact tcattttctg
     2821 tcgtttcaaa gtaaaatcgg tcccgtcaaa tggcttgaac cttcaactga ggtcacgctt
     2881 cggaaatttg ccgaagaacg ccgcaaacag gtgctggcaa ttccgatcag ctttgtttca
     2941 gaacatattg agaccctcta tgaaatggat atcctgtaca aggaaatggc agagaaaatc
     3001 cagttgccgt tctttcgggt acccaccctg aactgccatc ccttatttat tcaatcactg
     3061 gccgacctgg tggaaacccg gttacgcccc cagccacaac tggtggtcaa acaggctggc
     3121 cagcgataac tgttttatgc atcccctgcc ggagtaattt tccggtgccc aacgcagtcg
     3181 ctaaattgat tttttattcc gtccagggta aatttaagtc attcaaacat aacaaagttg
     3241 cgggctggca acaaaatcgc agacgatcac ctctctcatc acttgcgatg gcatacagtt
     3301 cgagaccata acggatgtga tgcgtttcct aaaactctgg ctcgtttctt gctcccaaca
     3361 caaattttaa ctggttcggc ttcaatgagg tcctaagcca ataacctaaa gaattctttt
     3421 tccagatagg ggaatttctc atgcaagacg tgattgtgat cggtggtggc atttctggat
     3481 tggtttgtgc atatcaactc aagcggcttg ggcgcaacgt cttgctcatt gacggaagca
     3541 accgtgtcgg cggggtcatg aaatccgagt caattgacgg ctttctattt gagcggggtc
     3601 caaacagttt ttacattacc ccggaaatct ctgaattact tgatagtatt ggaatttctc
     3661 cgtatctggt gaccgctgat agttccgctc cgcggtttgt ctactataaa ggcaagctcc
     3721 gcgccgttcc ggttccgacc tcccccttga agtttctgtc ttcatttttg accacccggt
     3781 tgctgtctcc actggccaaa ctccggctct ttgccgagcc atttattgcc cccagcacgt
     3841 ctggtcagga acaaaccatt gaggaattta tcacccgccg gtttggcaaa caatttcacg
     3901 atgtcctggt aagctctttt gtgtctggtg tttacgcagg agacacatct gaactcagtg
     3961 ccatggcatc gcctccgttt tcaaaactgg ctgaccttga agctgaacat ggcagtgtga
     4021 tccggggagc atttcgctcg aaaagaacgg ctgcagccaa agccggtgaa ccagctcgtc
     4081 gcgctcacca gagttgttca ttcctgaatg gattagagac cctgcccaaa gccctgcaca
     4141 aagccctggt aaatgaactg cttttaaatt gcaagattca cagcattgaa tttcacaatc
     4201 atgcgccgca agcccattat acacttgaag ttgaatttgg gggcgaccgc cagccaattc
     4261 atacagcctc gctcatcatt gcgacaccgg cgtacaccgc agcccggttg ctggcgccac
     4321 tttctgacaa actggccaat accctggatc aaattcccta tccgccactg gcctcagtgg
     4381 cactggcatt gaaagagtcc cagttaaagc tccaacctgg aacgtcactc cagggctttg
     4441 gctttctcat tccccggtca gaaggggttc ggacacttgg gtgtatctgg aactcctcgc
     4501 tgtttccaga gcgcgcacct caaggatatg tgactctcac ctgctttatt ggcggcagta
     4561 cggatcccga agtcctgaac ctgaatgatg acgacatcat tcggacagtt cataaagact
     4621 tgcagcaaac acttggagta agtggtgatc ctcgggtgtt aggggttacg cgatggaaac
     4681 gggccattcc tcagtacaca atcggccatc catcacgacg aactgaaatc gaagaacagg
     4741 taggccggta tccaggtctg tatctcaccg ggaattacat gtatggggtc tcaattggtg
     4801 at
//
LOCUS       DAOQDO010000138         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11699_length_4795_cov_2.743249, whole genome shotgun sequence.
ACCESSION   DAOQDO010000138 DAOQDO010000000
VERSION     DAOQDO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11699_length_4795_cov_2.743249"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1118
                     /locus_tag="PKZ53_03325"
     CDS             165..1118
                     /locus_tag="PKZ53_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNJ39495.1"
                     /translation="MKTLKSILLVAVVMVVLMMLSSPAFAQGVAGKWEGELNYGIYGV
                     ALDSTTYDWTFKKWSKQASIYKMVVELKHLEDEKFAGDYSLQSGTSAPGVFELHVTFN
                     DPAFKAETFGRLKSGSANPCTMELTLVNRKGKNYLEGKWRRTNTAAWEGVVSLLQTEG
                     GDAAAAPDPPAADPNAAPVEENETYVLHNKNGKTLHISEGKDGIRSSGNASDEQKRWK
                     FVHAPGKYARYYRIIPAGKDDHCLEIDPKDGSVHVRAIEDKDSGEQYWSLEKEGEFFF
                     LKCLGATGTQVLDNDNEENGNTIIRRKEDDATSQMWKVEKQ"
     gene            1265..3436
                     /locus_tag="PKZ53_03330"
     CDS             1265..3436
                     /locus_tag="PKZ53_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39496.1"
                     /translation="MKRTWLKKWLVILVFAAFMVNVVPAALAQTDPKPFDPNKNKPQE
                     WMNLPEIIPFANPDGGIDIAWSDHSAAPPKIYLTRFVKNADGYARSSAEELPSLGVLA
                     GFTKDEKGNVYHLTAELGKGEDPKRIVLYKNKQLFWEMKTQDGDGPPQSPKLPCDSGT
                     SQIVTGAGKLFVDINLLPAHAYNVILDLENPRANAQRYARETLWHHNFDQRVLFDGKD
                     FIAIENRDHEVTLSMMKFSPTESYPFSPYAERLRSVYTRTNNGNSTFTELGDIAVGAG
                     DGNGYLVLFASERDWDDQMAGLNKPGEQTGLNGHILPRDLAVIHVRKDFDRQEINWKS
                     IEAEREKDGNSISQAPKLVDTTSVVNSVGTGKIVSYKAENDGWDWPNYNADTAKLIKS
                     GDLAERAYKTGGVNWLTAYGDRFETAKRLPPVGKPFTTVANPKLVRVAPNNYVAIWEE
                     QNGTRIDGQPLTVAKTYQTTKAMGLTLEMQGSRVQITKSPINDLGKIRLMPFDDAFEL
                     GGKAAFVTGDEASKSLKLHVIDADLNYKAVTLPLTGDLKSAASKPVPPEPKTSPSSDV
                     KPAPATGFDFFELDGVGVKFARNWTNTRDRQARVISSTSPDKSVSLFLKGTEFTSYDP
                     VQEAIFDILEPLFPGLEALEEVNTEHDIFKGGLGLRLVTYTAAYKGDPVRISVEFAKD
                     TSDDAAPTVLLIRCVKEGDQKFESVISEVSQSLTKTNRRGK"
     gene            3606..4640
                     /locus_tag="PKZ53_03335"
     CDS             3606..4640
                     /locus_tag="PKZ53_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39497.1"
                     /translation="MKQRVKQVIACAATVGMLGMSGLTTLPAHAKTGNLGTPAVKKVH
                     KKPVLDLVINVFGKKTGFDPIQFGKTLSQVAGVYNIPLSKNTKKLFGGLTPTEPNPYR
                     MDGARIQHATYVSGAAASATVSPASFLQEEAAPSEEEEEVYTMVVTVGTDDADDDDDG
                     TLDTVDTDDDNDGFEDAKEETNVVDLDTLFEGEMAGFEDKDKAVGVYIEITDGEQTHE
                     IRLETDADSLEEQGEASARFEKYGVEVMKASFVGGTPVRTFGKLNRNDINTAVSIITT
                     FGSPVAKMGSQAAGAAGAKNAQANLLEASNVIKLADQSGLAKEGAKLAVQILNTNDAL
                     NKYNAMKPKK"
BASE COUNT         1340 a         1175 c         1229 g         1051 t
ORIGIN      
        1 atacctgcgc gaagaagcgc aggcggcagg tgcctcggcc tttgtatcga aatctgattt
       61 gttcctgctg cgatcaattg taacaaatca ttaaccctgg ccagagctgg ttctcttttg
      121 aacgagaccc cagaaatctt ttacttgaga tggagtgaag tgacatgaag acactgaaat
      181 caattttgct tgttgccgtt gtgatggtgg tcctgatgat gttgtcctcg ccagcttttg
      241 cccagggcgt ggctgggaag tgggaagggg aactcaacta tgggatatat ggcgtagctc
      301 ttgatagcac aacctatgac tggacgttta aaaaatggag taaacaggct tcgatttata
      361 aaatggtggt ggaattaaag catctggaag acgaaaagtt tgcgggtgat tattcgcttc
      421 agagtggtac ctcggcgccg ggggtgtttg aactccacgt taccttcaac gatccagctt
      481 ttaaagctga gacctttggc cggttaaaga gcgggagcgc caatccctgc acgatggaac
      541 taaccctggt taaccgcaaa ggaaagaact atttagaagg aaagtggcgc cggacaaata
      601 ctgcggcctg ggaaggggtt gtttctctgc tccagaccga aggtggtgac gcggcagccg
      661 cgccagaccc acccgccgcg gatcccaacg cggccccggt cgaagaaaat gagacctatg
      721 ttctgcataa caaaaatgga aaaacactgc acatctcaga aggaaaagat ggaatccgtt
      781 caagtggcaa cgcctcggac gaacaaaaaa ggtggaaatt tgtgcatgct cccgggaaat
      841 atgcccgcta ctaccgcatt attccggctg ggaaagatga tcattgttta gaaattgacc
      901 ccaaagacgg cagcgtccac gttcgggcaa tcgaagacaa agattctggc gaacaatact
      961 ggtcgcttga aaaagaaggc gaatttttct ttctcaaatg ccttggcgcc accggaacac
     1021 aggtcctgga caatgataat gaagaaaacg gcaacaccat catccgccgc aaagaagatg
     1081 atgccaccag ccagatgtgg aaagtggaaa aacagtaatt tcattgaagg gtcagctccc
     1141 tgatcgagag gtgtctaatc aaacggaaat gattgggata aaacaggata cgtttgggga
     1201 tgacactcaa aatcctgacg ctttcctgct ctttgctgac taaattgccc aaaaggagtt
     1261 gatgatgaaa cgcacctggc tcaagaaatg gctggttata cttgtattcg cggcatttat
     1321 ggtaaacgtg gtgccagccg ccctggccca aacagatccg aaaccatttg acccgaacaa
     1381 aaataaaccg caagagtgga tgaacctgcc ggaaatcatt ccctttgcca atcctgatgg
     1441 gggcattgat attgcctgga gtgatcattc agccgccccg ccgaagatct atctcacccg
     1501 gtttgtgaaa aacgccgatg gatatgcccg gtcgagtgct gaagaactcc ccagcctggg
     1561 tgtgctggca gggtttacca aagacgaaaa aggcaatgtc tatcacctga ctgccgaact
     1621 gggaaaaggc gaagatccca aacgaattgt gctctataaa aacaagcaac tgttctggga
     1681 aatgaaaact caggacggtg atggcccgcc tcagtcaccc aaactgccgt gcgacagcgg
     1741 gacgtcccaa attgtgaccg gcgccgggaa gttgtttgtg gatatcaatt tgcttccagc
     1801 acacgcctat aacgttattc tcgacctgga aaacccccga gccaatgccc agcggtatgc
     1861 acgggaaacc ctctggcatc acaattttga tcaacgggta ctctttgatg ggaaagattt
     1921 tatcgcgatt gaaaaccgcg atcacgaagt cactctctcg atgatgaaat tcagcccaac
     1981 cgaaagttac ccgttttcgc cctatgccga acgactgcgc tcggtgtata cgcgcaccaa
     2041 caacggcaac agcacgttca ccgaattagg cgacattgcc gttggagctg gcgatggcaa
     2101 cgggtatctg gtgttgtttg ccagcgaacg tgactgggat gaccagatgg caggtttgaa
     2161 caagcccggc gaacaaaccg gcctcaacgg acacatcctg ccccgtgacc tggcagtaat
     2221 tcacgtcaga aaagatttcg accgccagga aatcaactgg aagagcatcg aagccgaacg
     2281 ggaaaaagac ggaaactcaa tttcgcaggc gccaaaactg gtggatacga ccagcgtggt
     2341 caacagtgtt ggaaccggga aaatcgtttc gtacaaagcc gaaaacgatg gctgggactg
     2401 gccaaattat aatgccgata ccgccaaact gatcaaatca ggtgacctgg ccgagcgtgc
     2461 ctataaaacc ggtggtgtga actggctgac tgcctatggt gatcggtttg aaactgcaaa
     2521 acggttgcca cctgtgggga aaccctttac gaccgtcgcc aacccaaaac tggtgcgggt
     2581 cgctcctaat aattatgttg ccatctggga agaacagaac ggtacccgga ttgatggaca
     2641 acccctgacc gttgccaaaa cctatcagac aaccaaagcc atggggctca cgctggaaat
     2701 gcagggaagt cgggttcaaa tcaccaaatc cccgataaat gatctgggca agatccggct
     2761 gatgcctttt gacgatgctt ttgaactggg tgggaaagcc gcgtttgtca ccggtgatga
     2821 agcttcgaaa tcactgaaac tccacgtgat tgatgctgat ttaaactaca aagccgtgac
     2881 attgccattg actggcgatc tgaaatcagc ggcttcaaaa cctgttcccc cagaacccaa
     2941 aacgagcccg agttcagacg tgaaaccagc gcccgccacc ggctttgact tttttgaatt
     3001 agacggcgtc ggcgtcaaat ttgcccgcaa ctggaccaat acccgcgata gacaggccag
     3061 ggtgatcagc tcaacgagtc cggataaaag tgtatcgttg ttcctcaagg gaaccgagtt
     3121 tacttcttac gacccggtac aggaggcgat tttcgatatt ctcgaaccgc tcttccctgg
     3181 actcgaagcc ctggaagaag tgaacaccga acacgacatt tttaagggcg ggcttggact
     3241 gcgcctggtg acctatactg cagcgtacaa aggagatccc gtcaggattt cagttgaatt
     3301 tgcgaaagat acctcggacg acgcagcccc caccgtattg ttgatccgat gtgtaaaaga
     3361 gggagaccag aaattcgaat cggttatttc tgaggtgtcg caatccctga ccaaaacaaa
     3421 ccgccgtgga aaatagagtt tgacgggtct tggatccctc aattgatcaa ctgttgaacc
     3481 ttttcagata cctgatttca gcctggaatc acttttcatc acaaacattt gtcgtgtcag
     3541 cctgcttgag gcaactgccg ggaagcgaat gtgttttaac cagcttgaag gagagctaaa
     3601 agatcatgaa acagcgagtg aaacaagtta ttgcctgtgc cgcaaccgtt ggaatgcttg
     3661 gaatgtcagg tctcaccaca cttccggctc acgccaaaac cgggaatttg ggaaccccag
     3721 ccgttaaaaa agttcacaag aaacccgttt tggatctggt catcaatgtg tttgggaaaa
     3781 aaaccgggtt tgaccccata caatttggga aaaccctttc ccaggttgct ggtgtctaca
     3841 atattccgtt gtcaaaaaat acaaagaaat tgtttggtgg actcacccca accgaaccca
     3901 atccatatcg gatggatggc gctcggattc agcatgccac gtatgtcagc ggcgccgctg
     3961 ccagcgcaac cgtttcccca gccagttttc tccaggagga agcagccccc tcagaggaag
     4021 aagaggaagt ttacaccatg gtcgttaccg tcgggaccga tgatgcagac gatgacgatg
     4081 atggcacgct cgacacggta gacaccgacg atgacaacga cggctttgaa gatgccaaag
     4141 aagaaaccaa cgtggttgac ctcgacacgc tgtttgaagg tgaaatggct ggttttgaag
     4201 acaaagacaa agcggtgggt gtgtacattg agattaccga cggtgaacaa acccacgaaa
     4261 tcagacttga aaccgatgct gattcattgg aagaacaggg cgaagcttca gcccggtttg
     4321 aaaagtatgg cgtcgaagtg atgaaagcat catttgtcgg cggtacaccg gttcgaacat
     4381 tcggcaagct caatcgcaac gacatcaaca ccgctgtttc gattatcaca acctttggct
     4441 cacccgttgc caaaatggga agtcaggctg ccggggctgc cggagcgaaa aatgctcagg
     4501 ccaatctgtt ggaagcttca aatgtgatca aacttgctga ccaatccgga ctggccaaag
     4561 aaggcgccaa actggcagtg cagattctca acaccaacga tgcgttgaat aaatacaacg
     4621 ccatgaaacc aaaaaagtag gacagtttat tgtcagtagt ctgttacctc cattatgtca
     4681 gtaccacctg cgtgagcggg tgggtttgtt ggcttctcat cccacctgct cacgcgggtg
     4741 gtactgacag gtcctgtatt tccaacgcag ctttgacctt attgaacgac tgacg
//
LOCUS       DAOQDO010000139         4792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11709_length_4792_cov_2.477940, whole genome shotgun sequence.
ACCESSION   DAOQDO010000139 DAOQDO010000000
VERSION     DAOQDO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_4792_cov_2.477940"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKZ53_03340"
     CDS             <1..365
                     /locus_tag="PKZ53_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39498.1"
                     /translation="VSGFARCLREGNPLRLKGQGQQVRDILPAQELARAFQLFLRSDH
                     PGGVFNIGGGLEFSGTLLEFAHRIAVAQGLDPSLIAGIDTPPGTDEQQRYVSDLGKIG
                     CELGWTPEFDLDQTLRQV"
     gene            complement(394..1071)
                     /locus_tag="PKZ53_03345"
     CDS             complement(394..1071)
                     /locus_tag="PKZ53_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39499.1"
                     /translation="MPTRPVCCQPGSNPEHQLETAAQHRIQGSLIKSRLLFLHIKHGV
                     QTLKKILRVLPVEDQNLLCKTIYVGEWYSLATLVRLDLAIAAELGGQYSNVFEELGRF
                     SADLNLGGAYEPLMKKDIHALLNLSAVMNKTYQDFGKASYHQPEVSDPAGRQTVLLKL
                     EYPEPPPSHYCESGLGYFQHAVELCGGQDVTVEIIECQQQGGRCCCFKVQWEPPTDVS
                     LRPSSTR"
     gene            complement(1256..2164)
                     /gene="xerD"
                     /locus_tag="PKZ53_03350"
     CDS             complement(1256..2164)
                     /gene="xerD"
                     /locus_tag="PKZ53_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNJ39500.1"
                     /translation="MARDLGKAFLDYLRVERGLSANTLEAYGRDLEKLKGFSEAGQRD
                     LLTLERQDLVDFIQVLREGGLDWRSVGRVLVTVRNFYKYLLLDGFRKTDPTVHLPTPK
                     SASELPHFLTIEEVDRLLAQPEMSTEIGKRDRAILEVLYATGLRVSEVVTLKMVDVNC
                     EVGFLSCFGKGSKQRQVPLGKSAIEYLIQYWPVRARALKAIKEKGKAPGSELVFVTLQ
                     GKPMTRQGCWKMIVEYGKQAGIGHITPHMMRHSFATHLLEHGADLRSVQVLLGHSDIA
                     TTEVYTHVTNDRLKEVYKRFHPRAQS"
     gene            2351..2908
                     /locus_tag="PKZ53_03355"
     CDS             2351..2908
                     /locus_tag="PKZ53_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39501.1"
                     /translation="MPIKVENQASIQVKPDLEKLIQSVFDAVPKEHTRGISKVVLVDR
                     IQEKAIAREMREKLPCLYHPKVMGMPAWFEIALANFVEQKGLVKRLASRLNLKTNLAG
                     ATLSLIGQHYHFTLSHGLKPNQREKAVRTYIEKYFTVWRDLNSGWRARLFKPIYPYLD
                     RFGKWMSTHYQQQLRKQQSQAKPSK"
     gene            complement(2947..3789)
                     /locus_tag="PKZ53_03360"
     CDS             complement(2947..3789)
                     /locus_tag="PKZ53_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ39502.1"
                     /translation="MSKRGLPTTVKMRHEAHYVEELWQRTGAPIGRMIPIDRLEPNPN
                     QPRMEIGDLTELTASIREKGVLEPLLVRPSQVGGRFMIISGERRYRASLIAGLTELPC
                     IEMDVDDRAVAEIALIENLQRKDLTPFEEAEGLSTLVDRFGYTHDEVAKKIGKSRSSI
                     TEALSISSLSDDIKELCRAAKISSKSTLLQIVRQPGEDERRALIARIQSQGLTRDDVR
                     RVSKPPAQGRPRPFLFKFQSPAREFTLELKFKRSRVEREEVLTALRRIVEQLEQEGAK
                     EGDG"
     gene            complement(3782..4597)
                     /locus_tag="PKZ53_03365"
     CDS             complement(3782..4597)
                     /locus_tag="PKZ53_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ39503.1"
                     /translation="MKNKSLKSERKAAPVPAKGAATIAIANQKGGVGKTTTTINLAAA
                     LAMRGKRVLLLDLDPQGNSTLSYLDQSKIELSAYDFLTDLSIPAEIVIHRSPVEGLDI
                     LPARISLAKFETRLAGEFDAPFRLKDRLEAVNTRYDYILVDTPPTLGLITVNALVASS
                     HVLIPIQSSYFALEGTDDLLETVDKVKSRPNPNLNILGVVITLHDKRTTLSRDIFQQI
                     RQVFGERVFNTVITKSVRLEESPAYRESIFTYAPQSSGAIEYATLCEEVINRV"
BASE COUNT         1137 a         1378 c         1092 g         1185 t
ORIGIN      
        1 gggtctcagg gtttgcccgg tgcctgcggg aagggaatcc gttgcgctta aaaggacagg
       61 ggcagcaggt tcgagatatt ttgccggctc aggaattagc ccgagccttt cagttgtttc
      121 tgcggtcgga tcacccaggc ggcgtattca atattggggg aggccttgaa ttttcgggaa
      181 cacttcttga atttgctcat cgaattgccg ttgctcaggg cctggatcca tcgctcattg
      241 ctggtattga taccccccca ggcaccgatg agcaacaacg ctatgtctcc gaccttggaa
      301 aaattggttg tgagttggga tggacacctg agtttgatct tgatcagaca ctgagacagg
      361 tttagctggc agttccgttc cgctttgact cacttatctg gtggaactgg gtcgaagact
      421 gacatcggtc ggcggttccc actggacctt aaaacaacaa caccgcccac cttgttgctg
      481 acattcaata atctcaacgg tgacgtcctg accaccgcat aattcaacgg cgtgctgaaa
      541 ataccccaat ccgctttcac agtaatgtga tggaggcggt tcggggtatt cgagtttcag
      601 cagcaccgtc tggcggcctg ctgggtcgct gacttcgggc tggtggtatg aagctttgcc
      661 gaaatcctga taggttttat tcatgacagc agacagattc agcagtgcgt gaatgtcttt
      721 cttcatcagt ggctcatagg caccacctaa gttgagatcg gctgaaaacc gaccgagttc
      781 ctcaaagaca tttgaatact gcccgcccag ttcggcagca attgcgagat ccagccggac
      841 cagagtggcc agtgagtacc attcgccaac ataaattgtt ttgcagagta aattttgatc
      901 ctccactggt aacactcgga ggattttctt gagggtttga accccgtgtt tgatgtggag
      961 gaataacagt ctggatttaa tcaggctgcc ttgaatgcga tgttgagcgg cggtttcaag
     1021 ctgatgttcc ggattggaac ctggctggca gcaaacaggt cgggtaggca taggcaaaag
     1081 gctcgacttg gtttgattgg aatggtttag atgaaatcaa atcaaaggta ggttaactga
     1141 aagtaaccga aaagaaaagt taccacgtgg gctttaaatc tggatattga tctacataat
     1201 gtacattatc agactcaaaa agaatctgcg ctttcctgac tggtatcccc ttttcctatg
     1261 actgagctcg cgggtgaaat cgcttataca cctctttcaa tcgatcattg gtgacatgcg
     1321 tgtacacttc ggtcgtcgca atatcactgt gccccagcaa aacctgcact gaccttaaat
     1381 cagccccatg ctccagtaaa tgcgttgcaa aactatgccg catcatgtgc ggcgtaatat
     1441 ggccaattcc agcctgcttc ccatactcca caatcatctt ccagcagccc tggcgtgtca
     1501 tcggctttcc ctggagtgtg acaaacacca gctctgaacc cggtgctttt cctttctctt
     1561 tgattgcttt cagcgcccgc gcccgtaccg gccagtactg aatcaggtac tcaatcgctg
     1621 acttgcccag cgggacctgc cgctgcttgc tgccttttcc aaagcaactc aaaaacccta
     1681 cctcacaatt tacatccacc atcttaagcg ttaccacttc cgatacccgc agcccggtag
     1741 catacagaac ctccaggatc gcccggtctc gctttccaat ctcagtactc atctcaggct
     1801 gggcaagcag ccggtcaact tcctcgatgg tcagaaaatg cggcaattcc gaggctgact
     1861 ttggcgtcgg aagatgaact gtcggatcgg tcttccgaaa cccatccagc aacaggtatt
     1921 tataaaaatt tcgaaccgtg accagcactc gcccgactga ccgccagtcc agcccaccct
     1981 cacgcaacac ttgaataaaa tcaaccaggt cctgccgctc aagcgtcagc agatcccgct
     2041 ggcctgcctc gctaaacccc tttaacttct ctaaatcccg tccgtaggcc tccagggtat
     2101 tggccgataa cccccgctcc actcgtaaat aatccagaaa tgcttttcca agatcgcgtg
     2161 ccatccagct ttccctccac tctatgtgat ccaatcaggt tggtgttcca cgtgacactc
     2221 tgcatgtatt acttgaagta ccagagtctt actgcttttt ttcttcatac ttcagaagga
     2281 acagatcctt gaccccgccc ggttcctttc gtacactccc gcttttccac aaaggagtca
     2341 ttttccggtt atgcccatca aagtcgaaaa tcaggcgtcg atccaggtca aacctgacct
     2401 ggaaaaactc atccagagcg tgtttgacgc tgtccccaaa gaacataccc gcggtatcag
     2461 caaagttgtc ctcgttgacc gcattcagga aaaagccatc gccagggaga tgcgcgagaa
     2521 acttccctgc ctctaccacc caaaggtcat ggggatgccc gcctggtttg aaattgcgct
     2581 tgccaatttt gttgaacaga aaggtctggt caagcgactt gccagccggt tgaacttaaa
     2641 aaccaatctt gccggtgcca cgctgtccct catcggccag cactaccact tcaccctctc
     2701 gcacggcctg aagccaaacc agcgtgaaaa agcagtccgc acctatattg aaaaatattt
     2761 taccgtctgg cgagacctca acagtggctg gcgggccagg ctatttaaac caatctaccc
     2821 atacctggac cgcttcggta agtggatgtc aacgcactat caacaacaat tgcgcaaaca
     2881 acaaagccag gccaagccat ccaaatagct tctgcctggc caggtacatg tttttcgaac
     2941 cggggcttac ccgtcaccct ctttggcccc ttcctgttct aactgctcaa cgattcgccg
     3001 caatgcagtt aaaacttcct cacgctcgac ccgagaccgc ttaaatttta actccagggt
     3061 gaactcacgc gccggtgact gaaatttaaa taaaaaaggc ctcggtctcc cctgcgctgg
     3121 cggcttgctg acccgacgca catcgtctcg ggttaacccc tggctttgaa tccgggcaat
     3181 caatgcccgc cgctcatcct cacccggctg gcgcacaatt tgcaacaggg ttgattttga
     3241 agaaatcttt gccgcccggc ataactcctt gatgtcatcc gaaagcgaac taatcgagag
     3301 cgcctctgta atcgatgacc gggacttccc gatttttttg gcgacctcat catgcgtata
     3361 cccaaaccgg tcaaccagcg tcgataaccc ctcagcctcc tcaaatgggg tcagatcctt
     3421 gcgctgtaaa ttctcaatca acgcaatctc tgccaccgcc cggtcatcaa cgtccatctc
     3481 aatacagggt aactcggtta agcccgcaat caggctcgct cgataccgac gctcccccga
     3541 aataatcata aaccgcccac ccacctgact tggacgcacc agcaacggct ccaaaacccc
     3601 cttttcccga attgaggccg tcagttcggt caaatcgcca atttccattc ggggctggtt
     3661 tggattcggc tccaaccggt caatcggaat cattcgccca atcggcgctc cggttcgctg
     3721 ccacaattcc tcaacgtagt gtgcctcatg ccgcattttg acggttgttg gaagccctcg
     3781 cttagacacg attgattacc tcctcgcaca aggttgcata ttcgatggcc ccgctcgatt
     3841 gcggcgcata ggtaaaaatc gactcccgat aggccgggct ttcctcaagt cggaccgatt
     3901 tggtgatcac tgtgttaaac acccgctccc caaaaacctg ccgaatctgc tgaaaaatat
     3961 ctcgtgacag cgttgtccgc ttgtcatgca gcgtaattac cacccccaaa atattcagat
     4021 tcgggttggg ccgcgactta accttgtcaa ccgtttccaa taaatcgtcc gttccctcca
     4081 gcgcaaaata ggaggactga atcggaatca gaacatgcga cgatgccacc agggcattca
     4141 ccgtaatcaa ccccagggtt gggggcgtgt ccaccagaat gtaatcatac cgggtattga
     4201 ctgcttccag ccggtctttt aatcgaaacg gtgcatcaaa ctcgccggcc agccgggtct
     4261 caaacttggc cagactgatg cgggccggca gtatatccag cccttcaact ggactacggt
     4321 ggatcacaat ttcggctgga attgataaat cggtcaaaaa atcataggcc gaaagctcta
     4381 tttttgattg atcgaggtaa gaaagcgtgc tgttcccctg cggatccaga tccagcaaca
     4441 gaacccgttt cccacgcatg gccagggccg cggccagatt gatcgtggtc gtggtctttc
     4501 cgacgccccc tttctgattg gcaatcgcaa tcgtggcggc acctttggcg ggaactggcg
     4561 cggcttttcg ttcagatttc agagatttgt ttttcattgg gatctcgaac cagacctgga
     4621 actgattcca gtcagtatgc gacgaggcag tgtcgctcct ttctcttttc acagaagatt
     4681 tacattttgt accgatccat atccgattca tctatttctt cgggccactc gatttcctca
     4741 gctgaactct cacccggttc agcgctttca tccaaatctt tttccaccag gg
//
LOCUS       DAOQDO010000140         4768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11799_length_4768_cov_2.980480, whole genome shotgun sequence.
ACCESSION   DAOQDO010000140 DAOQDO010000000
VERSION     DAOQDO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11799_length_4768_cov_2.980480"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKZ53_03370"
     CDS             complement(<1..216)
                     /locus_tag="PKZ53_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39504.1"
                     /translation="MPGCDRKRVGIVAPATQAKTGQAGGKSATTSGQPIKAHLKQANP
                     LPCERPLFNAPRCLAFDAAGNLLIADEF"
     gene            816..4121
                     /locus_tag="PKZ53_03375"
     CDS             816..4121
                     /locus_tag="PKZ53_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39505.1"
                     /translation="MSGSKHWIWMLPVVLFVLTLSVFGQATDGAINGKVLDESGAVIS
                     GATVTVKHSETGFQTTATSDEQGQFRLNYLPVGKYIVTTEATGFKTTEVSNIAVILNQ
                     AIDLTIRLQTGEVNEVVTVSGGDVLVETSTSTLGNTFESRKVVELPSLNNSQLELALL
                     APNTTSQAGGTAGEGGSVGGNRPRNNSFTLDGVDNNDTILTGHVIDVIPEAVAEFTLL
                     TNQYTAEFGHSSAGQFNTITKSGTNEFHGGASWFNINKNYIAVDHLTKEAIARGDQPD
                     KKPRYDFNRIAGFLGGPVIKNKLFFFGAYQYNTTGTAGAPVSFLTPTAAGFAQLGSLS
                     GVSPFTLGLLQQYTVPASSQSDSVRVLGKDIPVGTVNFLNPNYLANHTFNVNVDQLLG
                     ADQIRYRYNYAKSTAPNAGVGNPIFNGNFTQLNQLASVTYIHSFTPTLVNEARIAYRR
                     KNNSFGVPDEFKSFANISFNDLGYIFGPQPESPSGEITNSYQLVNNLSWINGRHNLKF
                     GVELRNVISANSFLPRNRGEYDYATLEEYLLDRKPTGFNGGLKGVGTSVFAANRKSIY
                     WFVQDDFKISPTLTLNLGLRYEFYTIFRDEKLQALNAIASVPGVIEFREPKPDKNNFA
                     PRIGLAWSPSFSNRIGRMVFGENGRSSIRAGFGMSYDVAYGNLGTLQLPPQFQQELSS
                     AGGEGGPYGTSTNFLQNGGLGNTPIPPTTPADARAATQAFIPDKVLPYTMSWSLSYQR
                     QLSNDWGVELRYLGTRGVKQFAQIRSNGGNVPFAIAGFSLPTYLNASQVPDANTRDTM
                     PTLDQLLASSVYGTGVGGLGVADFSTPIVSQFLPVGSSTYHAASIDVTKRLSRGYTMS
                     AAYTFSHTIDFGTNDLFTSFINPRRAQDGFNLGDMRGNSALDRPHRFVTSIIWELPWF
                     RRNQNRLVRTLLGGFQFNTIYTAESGQPFTALSATDSNLNADTAGDRALRNPNGIKGT
                     GSNVTSILNSRGQVVGYLATNGNAEYIKAGLGVQPTAGANTLRSPGTNNFDISIFKDF
                     EIREGWRLQFRTEMFNAFNHEQLTVGGGSVFQLLTNARNLSYANVSSPTFNDSRIFAG
                     RPRVIQFGLKLIF"
     gene            complement(4504..>4768)
                     /locus_tag="PKZ53_03380"
     CDS             complement(4504..>4768)
                     /locus_tag="PKZ53_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ39506.1"
                     /translation="STDVILAEDLPETIIEWAPQSDSGEAADGYHDAVSEFKRKLIIK
                     AVADAGGNYTEAGKLLGLHPNYLHRLIRNLNIKDQLTRNSGKL"
BASE COUNT         1184 a         1311 c         1131 g         1142 t
ORIGIN      
        1 gaactcatca gcaattaaca gatttccggc tgcatcgaac gccagacacc gtggggcatt
       61 gaataatggt cgctcacaag gtaagggatt ggcttgtttc agatgagcct tgattggctg
      121 gcccgaggtt gtggctgatt tcccgcctgc ctgtccagtt ttagcctggg tagccggagc
      181 gacaatccca acccgcttcc gatcacagcc aggcacacca gaacgccaag ccagctcatc
      241 cgactttgac tgaaacgaat cctcatgcag cggtgctccc catcggttcg ggcaattgat
      301 tcaaattcca acaaccacaa gttggagctt tcaaaaaacg caattcccca gaaatgatag
      361 attcagatat gttgtttgat tgaatgaagt gtaggttaca cgcatttgca gtgcagatag
      421 agcttaacag ttcaattccg actgtcaagc aaaaacatag ttttatctct tttgcggcta
      481 tctgaatgct agcgcttcgc cgtgcaaaac caaaaaccga cacgtgaagc tgaaacccca
      541 taaacaccaa gataagagtt tgaaacactc aagagagtag tgtttccttg cgttgccggt
      601 tcatcccggc tgctatccgt ttgtcacaaa ttccctgccg cttctcccat tttcgaaggt
      661 gattcaggct cggcggccta cactcggcag cacaataagc agccacttcc acccagcaat
      721 tggattagtg ccggcatgag actaaaaatt ggtctggact gggcgggacg ggctgcttgt
      781 cacgttttca gacgctttga gcctggagga cacctatgtc aggaagcaaa cactggatct
      841 ggatgctccc tgtggtgctg tttgttttga cgctttcggt ctttggtcag gcaacggatg
      901 gagccatcaa cggcaaagtc ctcgacgaat ccggcgccgt tatttctggc gcgaccgtca
      961 ccgttaaaca cagcgaaaca gggtttcaaa ccaccgcgac ctctgatgaa caggggcaat
     1021 ttcgactcaa ttacctccct gttggcaagt acattgtaac aactgaagcc acgggattta
     1081 aaaccaccga agtttcaaat attgccgtga ttctcaatca ggcaattgac ctcaccattc
     1141 gcctccaaac cggcgaagtc aacgaagtcg tcaccgtttc aggtggcgat gtgctcgtcg
     1201 aaaccagtac ctcaaccctg ggcaatactt ttgaatcgcg caaagtcgtc gaattgccca
     1261 gcctcaataa ttcacaactt gaactcgcac tgctggcccc caacacgacg tcgcaagctg
     1321 gtggaaccgc tggcgaaggg ggttcggtcg gcggtaaccg cccccgaaat aacagcttca
     1381 ccctcgatgg cgtcgacaac aacgacacca tcctgaccgg tcatgtcatt gacgtgattc
     1441 ctgaagccgt cgccgaattt acgctcctca ccaaccagta cacggctgaa tttggacatt
     1501 cctcagccgg tcagttcaac accatcacca aatccggcac caatgaattc cacggtggcg
     1561 caagctggtt caacatcaac aaaaattaca ttgctgttga ccatctgacc aaagaagcca
     1621 ttgcccgtgg tgaccagcct gacaaaaagc cgcgctatga cttcaatcgg attgccgggt
     1681 ttctcggtgg cccggtcatc aaaaacaagc tgtttttctt tggtgcctac cagtacaaca
     1741 ccaccggaac ggctggggcg ccggtcagct ttttgacccc gaccgctgcc ggatttgccc
     1801 aacttggcag tctttccggc gtcagtccgt tcacgcttgg attgctccaa caatacaccg
     1861 ttccagcgtc gagccagtca gactcagttc gggtgcttgg gaaagacatc ccggttggaa
     1921 cggtcaattt cctcaatccg aattacctcg ccaatcacac ctttaacgtg aatgttgacc
     1981 agttactcgg tgccgatcaa atccggtacc ggtacaacta tgccaaatca acggcgccaa
     2041 atgccggagt cggaaatccg atttttaacg ggaactttac ccaactgaac cagcttgcct
     2101 cggtgactta tattcatagc ttcaccccaa cgctggtcaa cgaagctcgg attgcctacc
     2161 ggcgcaaaaa taacagcttt ggcgtgccgg atgagttcaa atcatttgcc aacatttcat
     2221 ttaatgatct ggggtacatt tttgggccac aacctgaatc ccccagcggt gaaatcacca
     2281 attcgtacca gttggtcaac aacctgagct ggatcaatgg ccgccataat ttgaaatttg
     2341 gtgttgaact gcgcaatgtc atttcggcca attccttcct gccacgaaat cgaggtgaat
     2401 acgactatgc cacgctcgaa gaatacctgc ttgatcgcaa accaaccggg tttaacggtg
     2461 ggttaaaagg cgtcggcacg tcagtgtttg cggccaatcg gaaatctatc tactggtttg
     2521 tccaggatga tttcaaaatt tcgccaacct taaccttgaa tttagggttg cggtatgaat
     2581 tttacaccat cttccgagat gaaaaactcc aggcactcaa cgccattgcc agtgtaccgg
     2641 gcgtgattga atttcgagaa ccaaaacctg acaaaaacaa ctttgcgcca cgtatcggcc
     2701 tggcctggtc accatccttt tccaatcgta tcggacggat ggtttttggt gaaaatggac
     2761 ggagctccat ccgagccgga tttggaatgt catatgatgt cgcctatggc aacctcggca
     2821 cgctccagtt accgccccag ttccaacaag aactttcctc agcgggtggt gaaggtggtc
     2881 cctatggcac ctcaaccaac tttctgcaaa acggtgggtt aggtaatacg ccgattccac
     2941 cgacgacgcc ggctgatgcc cgggcagcca cccaggcgtt tattccagat aaagtcctgc
     3001 cttacaccat gagctggagc ttgagttatc agcgacagct ttccaatgac tggggcgttg
     3061 aattgcggta tctgggaaca cgcggcgtca aacaatttgc ccagattcgc agcaatggcg
     3121 gcaatgtgcc cttcgcgatt gctgggttta gcctgccgac ctatctgaat gcctcccagg
     3181 tgccagatgc caacacccgt gacaccatgc caacgctgga ccagcttctg gcctcctcag
     3241 tgtatggaac gggcgttggt ggactcgggg tggctgattt ctcaactccg attgtgtctc
     3301 aattccttcc agttggcagt tcaacctatc acgcagcgtc cattgatgtg acgaaacggc
     3361 tttccagagg ctacacaatg tcagccgcct atacgtttag ccacaccatt gattttggga
     3421 ccaatgatct gtttaccagt tttatcaatc cacggcgagc gcaggatgga tttaacctgg
     3481 gtgatatgcg tggaaactcg gcccttgatc ggccacatcg gtttgtcacg tccatcatct
     3541 gggaattgcc gtggttccgg cgcaaccaga accggctggt ccggacgctg cttggcggct
     3601 tccagttcaa caccatctac acggcggaaa gcggtcagcc atttactgcc ctgagcgcca
     3661 ccgacagcaa cctcaatgct gacacagccg gagaccgtgc gcttcgcaat ccaaatggaa
     3721 tcaagggcac aggaagcaat gtgacctcaa ttctcaactc cagaggccag gtggttggct
     3781 atctcgccac caatggcaat gccgaataca tcaaagccgg actgggtgtc caaccaacgg
     3841 ctggggcaaa tacgcttcgg tctcccggaa ccaacaattt tgacatctcg atcttcaaag
     3901 atttcgaaat ccgcgaaggc tggcggcttc agttccgcac cgagatgttc aatgcattca
     3961 accacgaaca actcaccgtg ggtggtggga gcgtctttca actcctgacc aacgcccgca
     4021 acctgtcata tgcgaacgtg tcgagcccga cctttaacga ctcacgcatt ttcgcgggtc
     4081 ggccacgggt gatccagttt ggcttgaaat tgatctttta gtgaattgtt tgaaatcagt
     4141 catttttgaa caaaattcaa atgagtgttt tttcccgtcg aaacggctgt tcacttcaga
     4201 tgaatttccc tacaattttt tgtactgaag cctgtgtatc tgattgtgct tgtactttct
     4261 cgaaggtcac aggattgctc agtgccccgg acgaagcgtg gcgcctcatc cggggatttt
     4321 tatttttggg gttcggggtt tggggttccg ccagggttga gaaaaatatt aaaggacata
     4381 aaggacgaaa gggacgaaag ggacgaaaat gaaataatgc agttgctgtg agctgaaccc
     4441 aattcgaaaa ccccgaaccc ggaaccccga acccggaacc ccaaacccgg aaccccaatt
     4501 ctctcacaat ttcccactat tgcgggtcaa ctggtcctta atattcaggt tgcgaatcag
     4561 ccggtgcaaa taatttggat ggagcccaag caatttccct gcttcggtat aatttccccc
     4621 ggcatcagcc acagctttga taatcagctt tcgtttaaac tctgaaactg catcgtgata
     4681 gccatcggca gcctcaccag agtcagactg cggagcccat tcaataatcg tttccggcaa
     4741 atcttcagcc aggatcacat cggtgctc
//
LOCUS       DAOQDO010000141         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11813_length_4765_cov_2.209766, whole genome shotgun sequence.
ACCESSION   DAOQDO010000141 DAOQDO010000000
VERSION     DAOQDO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11813_length_4765_cov_2.209766"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1741)
                     /locus_tag="PKZ53_03385"
     CDS             complement(116..1741)
                     /locus_tag="PKZ53_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNJ39507.1"
                     /translation="MSSVAPPSDSRPAVRSGIQPQGDGIHPVAPPRSALQMFWENLLA
                     VPYRLRDTAFRGGTPNTDRTRSTFVFGNVFLHLHSVRTHRWSLRWATTAGLGIASLAA
                     FLITLITGVLLMFYYKPYPDVAYLSIKDIHFVVPTGRLIRNIHRWAANVMVVTVLLHM
                     ARTFFTGAYRKPREFNWLIGMGLLVTTLGLSFTGYLLPWDQLAYWAITIGANIAQSPR
                     EITDAVGVTGWFDPGGLNRLLLLGSDTVGPEALIRFYLLHIMVLPLMLTALMAVHFWR
                     IRKDGGLARPADADARVETPEGSVYPVFTQLATKTYQLAAIVKGKTSAVGNGPEGTVP
                     SMPHLFYAEMAVLMVTVLVCVALALITDAPLKELANPAVPENPAKAPWYFLGLQEMVS
                     FSAFMGGIGIPTIVLIGLGLIPFLDREESGTGEWFGGPGGWPLVKQSIWVGLISSIVI
                     DAFAIRFGWLREWIPHIPQLVITFINPGTVLTLVYALYSVWLVRRYNSTRAGALGLFT
                     CFLCGFIVLTIVGTYFRGPNWVFYWSPADWPKH"
     gene            complement(1738..2226)
                     /locus_tag="PKZ53_03390"
     CDS             complement(1738..2226)
                     /locus_tag="PKZ53_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ39508.1"
                     /translation="MTTKAREPRSRLDPEPIPRRDFLGLASLWAAASAMLFATLGMLQ
                     LPKTAVLSSPSKKFTVKLPDTLNPGEAFVPPGRAVALYKDASGGVYAVSTICTHLGCV
                     VKQTTEGFECPCHGSRFTSDGEVTKGPAPRALPWLQVTGNGTSITIDEGTTVESGTKV
                     TI"
     gene            complement(2223..4136)
                     /locus_tag="PKZ53_03395"
     CDS             complement(2223..4136)
                     /locus_tag="PKZ53_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ39509.1"
                     /translation="MDGLKVQVPHESYHQELISCQVACPVHTDARGYVRAIANGDFEA
                     AYLIARGPNPFASICGRICGAPCETACRRGKVPRVDDDGQFVAADRPVAIRALKRFAC
                     EQAGPDSHPTDVVLKKIQLAQPSVAANAEEMAALLRAGVEGKFVPAQGQRVAIIGAGP
                     AGLAAGHDLALMGFRPVVFETEPVAAGMLAVGVPAYRLPRDLIKREVAVIEALGVEIR
                     CNTTVGKDISFSDLRNDFDAVIVAVGAKSSRSLGLPGENGPRVYGGVDLLRAVSLGTS
                     LEIGQQVVVVGGGNVAYDVARSVLRQIAYDTARTAARLAATSLVHLVSLEGLEEMPAD
                     TVEIMEGDEEGIERWNGWGPVGILRNDEGLVTGVEFRRCLQVYDENRRFSPVYDDEDR
                     KVISCDTVLLAVGQSPALTFLEDGGTDVQMMRPGWPKVDPKTLASTADRVFVAGDLAH
                     GTRLLIDAVASGKAVARSVYQSLTGRSITFDAVSALLVLDRYRREQGYESIRRADIPT
                     LHPSERLLHPEAVVETGFTCAQAMGEASRCLDCGVTPIFDGSRCVLCGGCADVCPTQC
                     LKLVSLAELIPHDELNQAIEQTLGSDIDLTEHSAILKDEDRCIRCALCFMRCPAEAIT
                     MERVTTSISWRTL"
BASE COUNT         1169 a         1329 c         1272 g          995 t
ORIGIN      
        1 ccttgtcatt cggtcatctt gtcagggttc agggttttcg aatttatgtc cttttcgtcc
       61 cttatgtcct ttttgtccct ttggttgtta ccctgaaccc tgaaccctaa ttccatcaat
      121 gctttggcca gtccgctggc gaccagtaaa acacccagtt cggccctctg aagtaggtgc
      181 caacaatagt caggacaata aacccacaca aaaaacaggt gaagagtcca agcgctccgg
      241 cacgagtcga gttatatcgt cgaaccagcc ataccgagta cagggcgtac accagcgtca
      301 acaccgtgcc tggattgatg aaggtaatga ccagttgcgg aatgtgtgga atccattcac
      361 gcagccaccc aaaccgaatc gcaaaggcat caatcacaat tgaggaaatc aacccaaccc
      421 agatggattg tttgacgagc ggccagccgc caggaccgcc aaaccattca cccgtgcctg
      481 actcttcccg gtccaggaag ggaattaaac ccaatccaat caagacgatg gttggaattc
      541 caattccgcc cataaaggct gaaaacgaga ccatttcctg caagccgaga aagtaccagg
      601 gagctttggc cggattttca ggaacggctg ggttggccag ttccttgagc ggtgcgtcgg
      661 tgatgagtgc cagcgcgaca cagaccagca cggtcaccat caacaccgcc atctcggcat
      721 aaaagaggtg cggcatggat ggaacggtcc cttccgggcc gttgccaacg gctgaagttt
      781 tgcctttgac aattgccgcc agttgatagg ttttggtggc gagttgtgta aacactggat
      841 agaccgagcc ttccggagtt tcaaccctcg cgtccgcatc cgccgggcgg gccaatccgc
      901 catcttttcg gattcgccag aaatgcacgg ccatcaacgc ggtcagcatc agcggcaaca
      961 ccatgatgtg gagtaaataa aaccgaatca gggcttctgg gccgaccgtg tctgatccga
     1021 gcagcaacag gcggttgagt ccaccagggt caaaccagcc cgtgacgccg acggcatcag
     1081 taatttcccg tggcgactgg gcaatattgg caccgatggt aatggcccag tacgcgagtt
     1141 gatcccaggg caacaggtat ccagtaaacg aaagcccaag cgtcgtaacc agtaacccca
     1201 ttccaatcaa ccagttaaat tctcgtggct tgcgataggc gccagtaaag aatgtgcggg
     1261 ccatgtgcag gagcaccgtg accaccatca cattggctgc ccagcggtgg atgttgcgaa
     1321 tgagacgccc ggtcggcacc acaaaatgaa tatctttgat cgaaagatag gcgacgtccg
     1381 ggtaaggttt ataataaaac atcaacaaca cgccggtgat taatgtaata agaaacgcag
     1441 ccagactggc aatccccaac cccgccgtcg ttgcccagcg gaggctccag cggtgagttc
     1501 gcactgagtg taaatgcagg aaaacgttgc cgaaaacaaa tgtggagcgc gtccgatcag
     1561 tattgggtgt gccgccacga aacgcggtgt cccgcagccg ataggggacc gccagcaggt
     1621 tttcccaaaa catttgtaat gccgaacggg gaggtgcaac cggatgtatc ccgtcaccct
     1681 ggggctggat tccagaccgg acggctggcc gagagtcgga tggtggtgca acactgctca
     1741 tatcgtcacc ttggttccgg attcaaccgt tgtaccttcg tcaatggtta tgctcgtgcc
     1801 gtttccagta acttgaagcc acggcagggc ccggggtgcc gggcccttgg taacttcgcc
     1861 gtcactggtg aaccgcgagc cgtggcaggg gcattcaaac ccttcggtgg tttgtttcac
     1921 aacacacccc agatgtgtac aaattgtgga aaccgcatag acaccgccac tggcatcttt
     1981 atagagagcg acggcccggc ctggcgggac aaacgcctct ccaggattga gtgtgtcagg
     2041 cagtttgact gtgaatttct tggacggaga ggacaacact gcggttttgg gaagctggag
     2101 cattccaagc gtggcaaaca acatggcgga ggcggcagcc cacaacgaag ctaatccgag
     2161 aaaatcccgc cgtggtattg gttctggatc aagccgtgag cgcggctccc tggctttggt
     2221 tgtcatagcg tcctccagga aatagaagtt gtaacccgtt ccatcgtaat ggcttcagcc
     2281 gggcagcgca taaaacacag cgcacagcgg atgcagcggt cttcatcttt gagaatggct
     2341 gagtgttcgg tgagatcaat gtcagatcca agtgtttgtt caatggcctg attgagttcg
     2401 tcatggggta tcaactcagc gagtgacacc agtttgaggc actgagttgg gcatacgtcg
     2461 gcacagccac cacacaacac acaccggcta ccatcaaaaa ttggggtcac gccacaatcg
     2521 aggcagcgcg aggcttcgcc catggcctgg gcacaggtaa atccagtttc aaccacggct
     2581 tccgggtgga gcaggcgctc tgacggatgc agtgttggaa tgtcagcccg gcggatggat
     2641 tcatagccct gttcccggcg gtaccgatcc agcacaagga gggccgagac ggcgtcaaac
     2701 gtgatcgaac gtccggtcag cgactgatac accgaccggg ccaccgcttt ccccgaagcc
     2761 accgcatcaa tcagcaaccg tgttccgtgg gctaaatctc cggctacaaa cacccggtca
     2821 gccgtggacg caagcgtttt cggatcaact ttgggccagc cgggccgcat catctggaca
     2881 tcggttccac catcttccag aaacgtgagc gccggcgatt gaccaaccgc cagcaacacc
     2941 gtgtcacagg agatcacctt tcgatcttca tcgtcataca ccggtgaaaa tcgccgattt
     3001 tcgtcataaa cctgcaggca tcggcgaaat tcaaccccgg tcaccagacc ttcatcattg
     3061 cggagaatgc caaccggccc ccaaccgttc cagcgttcaa tcccttcttc gtcgccttcc
     3121 atgatttcga cggtatcagc gggcatttct tcaagtcctt caagcgaaac cagatgcacc
     3181 agcgacgtgg ctgcaagtcg ggcggcggtg cgggcggtgt cataggcaat ttgccgcagg
     3241 acactccggg cgacgtcata ggccacgttt ccaccaccaa ccaccaccac ctgttggcca
     3301 atttcaagac tcgtcccgag cgacaccgcc cggagcaagt caacgccgcc atacaccctg
     3361 ggaccatttt cacccggcag ccccagtgac cgcgacgatt tcgcgcccac ggccacaatc
     3421 acggcgtcaa aatcgttgcg caaatccgaa aaagaaatat ctttgccaac cgtagtgttg
     3481 cagcggattt caacgccaag ggcctcaatg accgcgactt cacgcttgat caggtcacga
     3541 ggaagccggt aggccggaac acccaccgcc agcatcccgg cggcaacggg ttcggtttca
     3601 aaaaccactg gtcgaaatcc catcaaggcc aggtcgtgtc cagccgccag tccagcgggg
     3661 ccggcgccga taatggcaac tcgctggccc tgagccggta caaattttcc ctcaacgccg
     3721 gcgcgtaaca gggccgccat ttcctcggca ttggccgcaa ctgagggttg agccagttga
     3781 atcttcttga gtaccacatc ggttgggtgt gaatcggggc cagcctgctc acaggcaaag
     3841 cgtttgagcg cccgaatggc aactggccga tctgccgcca caaactggcc gtcatcatca
     3901 acgcggggga cttttccccg ccggcaggcg gtttcacaag gtgcgccaca gatccgaccg
     3961 caaatcgaag caaacgggtt tggccctcgg gcaatcaggt aggcagcttc aaaatcacca
     4021 ttggcaattg cacggacata tccacgggca tcggtgtgga ctggacaggc cacctgacaa
     4081 gaaatcagtt cttgatgata gctttcgtgc ggtacctgga ctttgaggcc atccatataa
     4141 acctcattgg gctgaagact ttgggctgaa gacttcgggc tgaagacttt gggctgaaga
     4201 cctcgggctg aagacctcgg gctgaagact cgcaagctcg gggctgaaga agttgggctg
     4261 aagactttgg ggctgaaaga ctcgcaagct cggggctgaa gatattggaa tgaaaaaatg
     4321 tgctgaaaaa ctcggggtga aaaaatcagg cataggactt tggattgaag agagttggga
     4381 ttggttctac ccgttttctt cagctctgag cttgcgaatc ttcagcccca agcccgaagt
     4441 cttcaacccg aaattttcag caccctacgc ttgtacggaa caaacctgtt gaatcaaaga
     4501 gagtttcaaa agcggacttc tttgtccgcc agtactattt ccttttcagc gcctgacgac
     4561 aatgatgaat cttcatcata tttctgatga aactgatcaa ttggattaac aggttattgc
     4621 tgcttcacca aagagcaata atagtttatc catataaaaa taaatggttt atattattgc
     4681 tgatttataa ttcaagaatg tttcaggagg ccagccagtt ctgcccgacg aatggcttca
     4741 aggcgcgagt ggacattgaa tttct
//
LOCUS       DAOQDO010000142         4759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11839_length_4759_cov_2.778061, whole genome shotgun sequence.
ACCESSION   DAOQDO010000142 DAOQDO010000000
VERSION     DAOQDO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11839_length_4759_cov_2.778061"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1377)
                     /locus_tag="PKZ53_03400"
     CDS             complement(70..1377)
                     /locus_tag="PKZ53_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ39510.1"
                     /translation="MKRLFLFSLAVLFLNVTARAQEKPHAFVGAEIIPITGAPISNGV
                     LVVHKGKIVAVGPAKTTQIPADAERHEVSGKVLMPGLVDSHSHIGGPGGGDSSGPFHP
                     DTRVLDAINVRDAGIQKAQAGGITTVNVMPGSGHLSSGQTLYLKLRDGQTIDDLLIFD
                     ADGKMSGGLKMANGTNSIRPQPPFPGTRGKSAALVRDQFVKAQEYRDKVRRANGDREK
                     MPPRDLAMEALVEVLDGKRVVHYHTHRHDDILTVLRMSKEFGFKVVLHHVSEGWKVAK
                     EIAAARAPASIIVIDSPGGKMETMDASFANGGILDRAGVLTGFHTDDGITDSRFFLRS
                     AALAVRAGMSRDKALYGMTMANAKMLDLDQRIGSLEVGKDADFILLSGDPLSVYTKVL
                     ETYVDGTKVFDRKDPKDYLYATGGYGASRDQVMHLDCFDHDEE"
     gene            1618..3138
                     /locus_tag="PKZ53_03405"
     CDS             1618..3138
                     /locus_tag="PKZ53_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNJ39511.1"
                     /translation="MSTQPESPLKRDTIQMTVTELESQFESLRQRSGLVLGPLVFFSL
                     WFLPMSRLSPEAHRLAAILGLVVVLWITEALPLAVTGLLGPTLVIALGVAPAAQAFGP
                     FADPIMFLFLGAFILSRSIYLHGLDRRFAYWVLTNPLVGERPSRILFAYGGICCVISM
                     WISNTATAAMMFPIGVAIIRTLRDVKKDAVTDAYACAVMLMCAFSASVGGLATPVGTP
                     TNLIGLGFIERQLGRRVPFFEWMAFAIPIVVVMYLALFFYLNWLCPPGVKNLPGIRQR
                     FLSDRQQLGRLTPGERNTLIAFGITVVLWITPGILALTIGDADPVAKIYSQRVPESVA
                     ALIGACLLFLLPTNWKQQEFTLSVKQAFEIDWGVMALYGGGITLGQLAFSTKLAESIG
                     ASLTGVIPSGGAGLIAVSSTVAVLVSELTSNVSSANMVVPVVIALGGKAGFQAAIAAS
                     MASSLGFMLPISTPTNAIVYSSGYVPLRSMMKYGILLDVIGFIVIVLGTLLLVPAH"
     gene            3232..>4759
                     /locus_tag="PKZ53_03410"
     CDS             3232..>4759
                     /locus_tag="PKZ53_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39512.1"
                     /translation="MFSPIYRYHCLVSRWSQLVSFSKIIGMGCCFLLWLAVIPVQAQQ
                     VVSGTLRGTVVDESSALVNGATVRLTNQGTGQVRETQTSGDGFYTFPSVPVGTYALRV
                     IASGFKPSEAANVNVTIGAVSTIDFKLVVGDTGDVVVVDAADSVPLVETTRAGESTLI
                     NSRQVTNLPVNGRNFKDFITLSPNVFPARSPESNSFGNSISIAGQRTYDTQLNIDGAD
                     FVNPFFAEPAGGTRPPFTISLESVQEFQVITGGGSAEYGRTTGGSINVVTKSGTNDFH
                     GTAFWFFRNKTLTATDAFDREPTDFTQNQFGGNVGGPIVRDRLFFFTAVDVQRRKDPF
                     VANFGPGLENFLPELQGRTDANQNAAVVLGKIDWTPNTSNILNYRFNYSRFELTKRGG
                     GNTLGIGLDHFGTEKPVTQSHVAQYTRILSPKAVNEFRFQYLRENRQVIADNGGPEVQ
                     ILQEGLVFGPNLFQNVPFNINDKYQIVDHLSYTTGSHSFKFGTDINLTGANEIFKGGV
                     Q"
BASE COUNT         1059 a         1235 c         1239 g         1226 t
ORIGIN      
        1 tgaaaaactt tgtatagaaa aaatgatgct ttgaaagaaa agaaaaaaga attttttcaa
       61 catgcgttct tactcctcat cgtgatcaaa gcaatcaagg tgcatcacct gatcgcggct
      121 ggccccatac ccgcccgtgg catacaggta atctttcggg tctttgcggt caaacacttt
      181 ggttccatca acatacgttt ccagaacctt ggtgtagaca ctcagcggat ctcctgagag
      241 gagaataaaa tcagcatctt taccaacttc gagcgagcca atacgctgat ccaggtccag
      301 cattttggcg ttggccattg tcattccata cagtgccttg tcacgtgaca tgcctgcgcg
      361 aactgccagc gcggctgaac gcagaaaaaa gcgggaatcg gtaatcccat catccgtatg
      421 aaacccagtc agaactccag cccgatccag aatgccacca ttggcaaatg aggcatccat
      481 cgtttccatt tttccgcctg gactatcaat cacaatgatg gatgctggtg ccctggcggc
      541 tgcaatttct tttgcgactt tccaaccttc gctcacgtga tgcagaacca ctttaaatcc
      601 aaattctttt gacattcgca acaccgtcaa aatgtcatcg tggcggtggg tgtgatagtg
      661 tacaacccgt tttccgtcaa gaacttcgac gagtgcttcc atggccaggt cacggggcgg
      721 cattttttca cggtcaccat tggcccgacg cactttgtcg cggtattcct gggctttgac
      781 aaattgatca cggacgagtg ccgctgattt gccacgtgtt ccagggaagg gaggctgagg
      841 gcgaattgaa ttggtgccgt ttgccatctt caatccacca ctcatctttc catcggcgtc
      901 aaagatcaaa agatcatcaa ttgtctggcc atcccgcaat ttcaggtata gggtttgacc
      961 actggacagg tgacccgagc ctggcatgac attcaccgtg gtgataccgc ctgcctgggc
     1021 tttttgaatg ccggcatcgc gaacgttaat ggcatccagc acccgtgtat ccgggtgaaa
     1081 tgggccgctg ctatcgccac caccaggacc accaatgtgg ctatgcgaat caaccagacc
     1141 gggcatcagc accttgcctg atacctcgtg acgttcggca tccgctggga tttgggtggt
     1201 tttggccgga ccaaccgcaa caattttacc tttgtgaacc accagcaccc cgttggagat
     1261 gggggctccg gtaattggaa tgatttcagc cccaacaaac gcgtggggtt tttcctgggc
     1321 gcgagcggtt acgttcaaaa acagcacggc cagactgaag aggaaaagac gtttcatcaa
     1381 aatacctcgt gagggaaaaa ctattctgag ggtggagtgt ttctttctat agaactgcga
     1441 gcggcacaca gcatttttga aatagaggtg gcattatagc gccatcgtca attggggact
     1501 gggggcctgg gaaaagatca caaagacaga aaagacgaag aggacgaaag aaagaagtca
     1561 ttcaaaaccc ctgaccccat tctctatccc ctatccccta tcccctatcc cctatctatg
     1621 tctacccaac ctgaatcccc actcaagcgc gataccatcc agatgaccgt gaccgaactt
     1681 gaaagccagt ttgagtcact tcgccagcgg agtggcctcg tgctcggccc actcgtcttt
     1741 tttagcctgt ggtttttgcc tatgtcgagg ctgagtccag aagcccatcg gttagccgcc
     1801 attcttggcc tggtggttgt gctctggatt actgaagccc tgccgctggc cgtcaccggg
     1861 ttgcttggcc caacgctggt gattgcgctt ggcgtggcac ccgccgcaca ggccttcggg
     1921 ccatttgccg acccgatcat gttcttgttt ttaggggcgt ttattctttc gcgctcgatt
     1981 tacctgcacg ggttggatcg gcggtttgcc tactgggtat tgaccaaccc actggtcggt
     2041 gaacgtccga gccggatttt gtttgcctat ggtggcatct gttgtgtgat ttcgatgtgg
     2101 atttccaaca ccgccaccgc cgccatgatg tttccaatcg gtgtggccat tatccgcacg
     2161 cttcgtgatg tcaaaaaaga tgccgtcact gatgcctatg cctgcgctgt gatgttgatg
     2221 tgtgcttttt cagcttcagt tggcggactg gccacgccgg ttgggacgcc gaccaatctg
     2281 attgggcttg ggtttatcga gcgccagctt ggtcggcgag taccgttttt tgagtggatg
     2341 gcgtttgcca ttccaattgt ggtggtgatg tatctggcgc tttttttcta cttgaactgg
     2401 ttgtgtccac cgggagtgaa gaatttgccc ggcattcgac aacgatttct ttccgaccgc
     2461 cagcagcttg gccgtctgac gcctggtgaa cgtaacacct tgattgcctt tgggataacg
     2521 gttgtgttgt ggatcacgcc tggtattctg gccctgacaa ttggtgatgc ggatccagtt
     2581 gcgaaaatct attcccaacg tgttccagaa agcgtggcgg cactgattgg tgcctgcctg
     2641 ttgtttttgc tgcctacaaa ctggaagcag caggaattta cactctcggt caagcaagcc
     2701 tttgaaattg attggggggt gatggcgttg tacggcggcg ggataacgct tggccaactc
     2761 gcattttcaa ccaaactggc cgaatccatc ggtgccagtt tgaccggcgt tattccttct
     2821 ggcggtgccg ggttgattgc cgtgtcgtca acggtggctg tgttggtctc ggaactcact
     2881 tcaaacgtgt cgtccgccaa catggtcgta cccgtggtga ttgcgcttgg tggaaaagct
     2941 ggtttccagg cagcgattgc ggcctcgatg gcttcgtcgc ttgggtttat gctcccgatt
     3001 tcaactccaa ccaatgccat tgtctatagc tcaggctatg taccattgag aagcatgatg
     3061 aaatatggaa ttctgctcga cgtcattggg tttatcgtca tcgtccttgg aacgcttctt
     3121 cttgttcccg cacattaatc cgtctttcaa tgaaaaaata ccatttcgaa aagaagggtg
     3181 agagttcagg gttcagcctg aaccctcgcc ttcgtacagg agaatctagt catgttttcg
     3241 ccaatataca ggtaccactg cctggtttcc cgatggtctc aactggtgag tttttccaaa
     3301 ataatcggga tggggtgttg tttcctgctc tggctcgccg tgattccggt tcaggcccag
     3361 caggttgtaa gtggaaccct tcgcggaacg gtcgtggatg aaagcagcgc gctggtcaat
     3421 ggcgccacgg tccgtctgac caaccagggc accgggcagg ttcgcgaaac ccagacatca
     3481 ggtgatgggt tctatacgtt tccatctgtt cccgtcggca cctatgccct gcgagtgatt
     3541 gccagtggct ttaaaccgag cgaagctgcc aatgtgaatg tgacgattgg cgctgtttca
     3601 accattgact tcaaactggt agtgggtgat accggcgatg tggtggtggt ggacgcggct
     3661 gattccgtcc cgctggttga aaccacgcgg gccggagaat caacgctcat caacagccgc
     3721 caggtaacca atctcccggt caatgggcgc aactttaagg atttcatcac cctctcgccc
     3781 aatgttttcc cggcgcgaag cccagagagc aattcatttg ggaattcgat ttcgattgcc
     3841 gggcagcgaa cctatgacac acaactcaat attgatggtg ctgatttcgt taacccgttc
     3901 tttgccgaac ctgccggagg aacacggcct ccgtttacga tcagcctgga atcggtgcag
     3961 gaatttcagg taattaccgg tggcgggtct gccgaatatg ggcgcaccac tggcggttcg
     4021 atcaatgtgg tgaccaagtc aggcaccaat gattttcacg gcacggcctt ctggttcttt
     4081 cggaataaga ctctaaccgc aactgatgcc tttgatcgtg aaccgacgga tttcacccaa
     4141 aatcaatttg gtggcaacgt cggtggaccg attgtgcgcg acaggctgtt ctttttcact
     4201 gccgttgatg ttcaacgtcg gaaagatccg tttgtggcca atttcggccc tggattggaa
     4261 aacttcctgc cggaattgca gggccggacg gatgccaacc agaatgcagc cgtggtgctt
     4321 ggtaaaattg attggacacc aaacacgagc aatatcctga attatcgctt taattacagc
     4381 cgatttgaac taaccaaacg tggcggcggc aacacgcttg ggattgggct tgaccacttt
     4441 ggaactgaga aaccagtcac tcaatcgcac gtggcgcaat acacgcggat tttatcaccc
     4501 aaagccgtca acgaatttcg ctttcaatac ttgcgcgaaa atcggcaggt gattgccgac
     4561 aacggtggac cggaagtcca gattctccag gaaggtctgg tgtttgggcc gaacctgttc
     4621 caaaacgtgc ccttcaacat caatgacaag tatcagattg ttgatcatct gtcatacacg
     4681 actggttcgc attcgttcaa atttggaaca gacatcaacc tcaccggcgc caatgaaatc
     4741 ttcaaaggcg gcgtccagg
//
LOCUS       DAOQDO010000143         4739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11924_length_4739_cov_3.665884, whole genome shotgun sequence.
ACCESSION   DAOQDO010000143 DAOQDO010000000
VERSION     DAOQDO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11924_length_4739_cov_3.665884"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1324)
                     /locus_tag="PKZ53_03415"
     CDS             complement(53..1324)
                     /locus_tag="PKZ53_03415"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNJ39513.1"
                     /translation="MTEHKTQRAAIIILLLTALALGVIWFQKSNQNGSDSSGVTAGID
                     PQAKQLFGEMTAAYQKLGSFSVTITTEVADSTDASLPTVRNSFTSEVLLRRPDQATVK
                     IKGKESETQAVGDGKFLYGIKNDTPNQFIKEAVPPMEGGNGILATIMFANQLSGEGNV
                     GPSPFVLSGLDLMQGLSEQMKSLSFGPPTTIDGVSAKTIIVVQSAPEGESKLTFVIGE
                     ADHLLRQVTFEHSVQGRIAGQMTETYTRLQPAPAITEQTFSFTPPAGAQQVAAVQALQ
                     NSKYKPGTLPIPINATDLAGNPVSLDQFKGKVVLLDFWATWCAPCRAELPNVIAAYTK
                     YQKQGFEVLGISLDNANAQAALTQFIKENKMPWRQIYEGKGWEGTISSQYQIRSIPFT
                     LLLGRDGRIAAVNVRGATLEPAIQQALTQKG"
     gene            complement(1559..3682)
                     /locus_tag="PKZ53_03420"
     CDS             complement(1559..3682)
                     /locus_tag="PKZ53_03420"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNJ39514.1"
                     /translation="MASYKTTIIDTSTIVIDRDVLQDFKVASSQEWLDTNGIGGFASG
                     TLARVRTRRYHGLLFAATKPPVVRKLLLNAFESSVKIDGKRYETSTNQYPDTVYPTGY
                     KYLVRFRNTPWPTWTYRLGEVEIEACLWMVQGENTTVVEYRLTSPPDVPVALELRPIL
                     AFRDYHSLTFENPNLEEKVSVDEGLITLTPYADMPPLYLAHNATEVSLEGFWYRRFQY
                     REELARGSDFQEDGYSPLVFSFHLNFGPAVVIASTNPHQIQEWEAMHDREMVRRFGVP
                     TSVLSSGVQADAKVRPLDASMPENRAQRAEALSADPLTRPLATATSPFIVSRGNNLET
                     VIAGYPWFTDWGRDTFISLTGLTLVTGRYATARNILKAFAEVEQNGLIPNRFQDGDEL
                     PEYNTIDGTLWFVYAIGRYLDYTNDLTFVAEELYPTLQNIFRHHLAGTNYNIHTTEDG
                     LLYGGQDGVQLTWMDAKIGNWVVTPRIGKPVEIQALWYNGLRVAASIAQALGDTEMAA
                     HYSDTASKAKASFAEKFWNHSRGCLFDVITDQQADAALRPNQIYAMGLPYPIFDDTER
                     CLTALRQVEQELRTPVGLRSLGPKEPGYTGRYEGDQRKRDAAYHQGTVWPFLMGGFVS
                     AWIRNQPNQPEAVAQARQWLSGFETHLKEAGIGSISEIFDGDAPHTPRGCIAQAWSVA
                     EVYRCLVEDIYGNCPPVWGTKQNEE"
     gene            complement(3883..>4739)
                     /locus_tag="PKZ53_03425"
     CDS             complement(3883..>4739)
                     /locus_tag="PKZ53_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNJ39515.1"
                     /translation="QTRYQLGHALIVYGIAVAFPGMVFPIIFLSLVGGAIWGITSPKT
                     IPPESSSIISDDLHPYLHRMIRVIALSTGVLIVIGLAGLGVHALVNNGSVADSFQRFS
                     NWLFHHRLLSLAAIALKLGTFTFGGGYVMVPLMEHEVVTTHQWLSHREFMDGMALGQL
                     TPGPVVITVTFIGYRVAGLPGALIATIGVFLMPFFFTVWAGKSLELFHRNRMVRGALA
                     GVTPTAIALLAAAATSLGLTALVGPPLSQLVVISIIALSAAIFVRCKFNPLYILLAAT
                     GVGWFFAP"
BASE COUNT         1170 a         1195 c         1239 g         1135 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac cctcatccct
       61 tttgggtcaa tgcctgctga atggcaggtt caagtgtcgc cccacgtacg ttgacggcgg
      121 caattcttcc atctcgaccc aaaagcaggg taaacgggat gcttcggatc tggtattggg
      181 atgaaatggt cccttcccaa ccttttcctt cataaatttg tcgccagggc attttgtttt
      241 ctttgataaa ttgagtcaaa gcggcctggg cattggcatt atcgagggaa atccccagga
      301 cctcaaaccc ttgcttttga tatttggtgt aagcggcaat cacgtttgga agctcagctc
      361 gacagggagc gcaccaggtt gcccaaaaat cgagcagtac caccttgcct ttgaattgat
      421 cgagtgaaac cgggttgcca gccagatcag tcgcattgat cggaatcggc agggtgccag
      481 gtttatattt tgagttttgg agtgcctgaa ctgctgccac ctgctgtgcc cccgccggcg
      541 gggtaaatga aaatgtttgt tccgtaatgg ctggtgccgg ttgcagccgg gtataggttt
      601 cggtcatctg gccggcaatc cgaccctgaa ctgaatgttc aaacgtaacc tgtcggagca
      661 ggtgatcggc ttcgccaatc acaaatgtca gttttgattc accttctggc gctgactgca
      721 ctacaataat ggttttggcc gaaacgccat caatcgttgt gggtggacca aaggagagtg
      781 atttcatttg ctctgacagt ccttgcatca gatcaagccc ggagagcaca aaaggagagg
      841 gaccgacgtt cccttcgcca gaaagctgat tggcaaacat aatcgtggcc agaataccgt
      901 taccgccttc catcggggga actgcctctt tgataaactg gtttggggta tcatttttta
      961 tcccgtacag aaacttccca tccccaactg cctgggtttc agactctttt cctttaatct
     1021 tgacggttgc ctgatcaggc cggcgcagca atacctctga cgtaaacgaa tttcgaactg
     1081 ttggcaagct ggcgtcagtc gaatcagcga cttcggtcgt gatcgtgacc gaaaaagacc
     1141 ccagtttttg gtaggccgct gtcatttcac caaacagttg tttggcctgt gggtctatac
     1201 cagcagttac accagatgag tctgatccat tctggttact tttttgaaac cagataacac
     1261 ccagcgcgag ggcggtgagc agcaaaatga tgattgccgc tcgttgtgtt ttgtgttcgg
     1321 tcattgaata ctccacagaa aaggcagggt gaagaagaat gaagaattga gaatgaagaa
     1381 tgaaaaaaca atgtggtgac tggtgtttag tgcttgatgc tttggggcca atgtcttcga
     1441 ggaaccagga accaggaacc acattgtttc ttcattctca attcttcatt ctcaattctt
     1501 cattctcaat tcttcattct tcattcttca ttcttcattt ttcttcattc ttcattcttc
     1561 attcttcatt ctgctttgtt ccccacactg gcgggcagtt gccataaatg tcttcgacca
     1621 ggcaccggta gacttcggca acgctccagg cctgggcaat gcagccccgt ggtgtgtgtg
     1681 gggcatcacc gtcaaagatt tctgaaatgc tgccaatacc agcttctttc aggtgggttt
     1741 caaaccctga aagccactgg cgggcctggg caacggcttc aggctggttt ggctgatttc
     1801 gaatccaggc ggaaacaaag cctcccatca gaaatggcca gacggttccc tgatgatagg
     1861 cggcgtcccg ttttcgttga tcgccttcat atcgtccagt atatccaggc tcttttggtc
     1921 ccagcgagcg cagtccaacc ggtgtccgga gttcctgttc aacctgacgt agcgctgtaa
     1981 gacagcgttc ggtgtcatca aaaatcggat atggaaggcc catggcatag atttgattgg
     2041 ggcggagtgc ggcgtcagct tgttgatctg taatcacatc aaacaggcat ccacgagagt
     2101 gattccagaa tttttcggca aaactggctt tggccttgct ggcagtatcg ctgtagtgcg
     2161 ctgccatttc tgtatcacca agtgcttgag caattgatgc cgcgactcgc aggccgttat
     2221 accagagcgc ctggatttca accggtttac caatccgagg cgtgacgacc caattgccga
     2281 ttttggcgtc catccaggtg agttggacgc catcctgacc gccatacagc aacccgtctt
     2341 cggtggtatg aatgttatag ttggtgccgg ccaggtggtg acggaagata ttttgcagcg
     2401 tcggatagag ttcctcggcc acaaacgtga gatcattggt atagtccaga taccgcccaa
     2461 tcgcatatac aaaccagagc gtgccatcaa tggtgttgta ttccggcaac tcgtcgccat
     2521 cctgaaaccg gttgggaatc aatccgttct gttcaacttc agcaaatgct ttcaaaatat
     2581 tgcgggcggt tgcatatcgc ccagtcacca gcgtcaagcc agtcaatgag ataaaggtat
     2641 cacgccccca atcggtaaac cacggatatc cagcaatgac ggtttccaaa ttgtttcctc
     2701 gcgaaacaat aaagggtgac gtggccgttg ccagggggcg ggtcagcgga tcggctgaca
     2761 atgcctctgc acgctgggca cgattttctg gcattgatgc atcgagtggc cggactttgg
     2821 cgtcagcttg aactcctgaa gataagaccg aagttggaac tccgaaccgg cgaaccattt
     2881 cgcggtcatg catggcttcc cattcctgga tttgatgggg attggtcgag gcaatcacca
     2941 cggctgggcc aaaattaagg tgaaaactaa aaacgagcgg cgaatacccg tcttcttgaa
     3001 aatcacttcc acgggctaat tcttcccgat attggaaccg gcggtaccaa aagccttcca
     3061 gtgagacttc ggtggcattg tgagccaggt agagtggggg catgtcagcg tagggggtca
     3121 gggtaatcaa tccttcatcc acgctgacct tttcttccag gttcggattc tcaaaggtca
     3181 gactatggta gtcacgaaag gccagaatcg ggcgcaattc gagtgccacg ggaacgtccg
     3241 gaggggaagt cagccgatat tcaaccactg tggtgttttc accctgaacc atccagagac
     3301 aggcttcaat ttctacttca ccgagccgat aggtccaggt tggccagggc gtgttacgga
     3361 accggaccag gtatttataa ccggtggggt agaccgtatc cgggtactga ttggtggagg
     3421 tttcatagcg tttcccatca atcttgactg atgattcaaa ggcgttgagc aggagttttc
     3481 gaaccactgg gggtttggtg gcggcaaaca acaggccgtg gtaccgtcgg gtgcgcaccc
     3541 gcgccagcgt gcctgatgca aaccctccga tgccattggt gtcgagccat tcctgggagg
     3601 atgccacctt gaaatcctga agtacgtcgc ggtcaatcac aatggttgag gtgtcaatga
     3661 tcgtagtttt ataggaagcc ataatttgat gatctcacag gatggacaaa tgtgactggt
     3721 gattgaggaa tgtgagtcag cgacacacac caccgaagca atcagaaaaa gggtgaccag
     3781 caaacccaaa tcacatcccc ccaaggtcat cgggtccctc aggacagacc cttatgccca
     3841 acagcggatc agtcaccagt ttcctgggtg tgttcaagat ttttaagggg caaaaaacca
     3901 accgactcca gttgctgcaa gtaagatata aaggggattg aacttgcatc ttacaaagat
     3961 tgcagcacta agcgcaatga tggaaataac caccagttgc gaaagaggtg gtccgaccag
     4021 agctgttaac ccaaggctgg tggcggcagc ggccagtaac gcaatcgccg ttggcgtgac
     4081 acctgccagg gcgccgcgaa ccatccgatt tcggtgaaac agctcaaggg atttaccggc
     4141 ccagaccgtg aagaaaaatg gcatcaggaa aacgccaatg gttgcaatca gagcgcctgg
     4201 taacccggca acccgatatc caataaatgt gacggtaatg acaactggtc cgggtgtgag
     4261 ctggcccaaa gccatgccat ccataaattc acggtgggaa agccactgat gggttgttac
     4321 gacttcgtgt tccatcaacg gcaccatcac ataccctccg ccaaaggtaa acgtcccaag
     4381 cttgagagct atggccgcca acgataagag ccgatgatga aacaaccagt tcgagaaccg
     4441 ctgaaaggag tcggccactg acccgttgtt gacaagggca tggactccaa gccctgccag
     4501 tccaatgacg atcaacacac ctgtgctgag agcaatcacg cgaatcatcc gatggagata
     4561 tggatgcagg tcgtcactga tgatcgaact tgattcaggc ggaattgttt tgggtgatgt
     4621 gatcccccag atggcaccac cgacaagcga tagaaaaata attggaaaca ccatgcccgg
     4681 aaacgcaacc gcaattccat acacgatgag ggcatgaccc agttgatatc gggtttgga
//
LOCUS       DAOQDO010000144         4736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11936_length_4736_cov_2.773980, whole genome shotgun sequence.
ACCESSION   DAOQDO010000144 DAOQDO010000000
VERSION     DAOQDO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_4736_cov_2.773980"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(433..1182)
                     /locus_tag="PKZ53_03430"
     CDS             complement(433..1182)
                     /locus_tag="PKZ53_03430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNJ39516.1"
                     /translation="MQNTYVFGDIHGRYRQLRRIVESLDYNPRRDRIIFVGDLIDRGE
                     DAPGVVSYVIELQAQNPRVVALRGNHEQMLLDLLDEGDPLWLIPENGGIMTMNQYGCE
                     INEETSTISLHIPQEHLDFFRRMPFFYEDDKALYVHAGIVPGKHPSECEPDVLIWTRD
                     LKFFKLYRGKPCFFGHTPTRYLPQDGVRQPGEIYVCGSAIGLDTGCTFEDPLSCFHVE
                     SGTVVRMYPDQDLTKYQVQVNCLDQVLEPKS"
     gene            complement(1505..3943)
                     /locus_tag="PKZ53_03435"
     CDS             complement(1505..3943)
                     /locus_tag="PKZ53_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNJ39517.1"
                     /translation="MPIQALETLEFPHLRVMLAREAKSEQGRLLALELTPTDEWPTVT
                     RNLTLTTEARRFSQEVGRLSVGDLPDPTSILEQLRIAGTRLETDQLQTVLRFMQVGIS
                     LQEMFRPVRDEFPTLNSVFHLLPNLSKLYQTTRRILLPTGEIDERASSELRRIRQEIQ
                     HQRARIHRQLEGMLRQAELEPAFSDQIITQRNGRFVLPIRSDHRGHLPGVVHAASSSG
                     ATVFIEPIGTIELNNEMVRLQDMEEAEIARILFQITEALRTEHKAIEQLTWTLAEIDF
                     LAAKARFAETFNAVEPKLNTDGVFSVIDARHPLLESRLLTEGGKIVPISFTLNSADQV
                     MIISGPNAGGKTVVLKTAGICALMAQSGLHVPAREANICLFHQIHADIGDHQSLAANL
                     STFSSHITNIRTITETVAAPALVLIDEIGTGTDPDEGAALGTAIVDFLRSQTIHTIVS
                     THYNPLKVYADTTDGVFNASVEFDFETLQPTYRLLTTQAGASSGIEIARRLGISPAIL
                     DLATQKLGSRERDLSHYLEKLKSEIDYHHDSNVALEEERATLAERYARLDHQFAEREL
                     ARQKVFTETLNELRDEFFEQVRPLLTQISDQVQRSKAQHSAERAVEKLARAANEKFKQ
                     QNALRLVKPTPPPTMPQLPEPARTEEPGVEEGELRVGDLVRTDLGQEGIIESIQQGML
                     LVRVGSMRLKTASTHVRRISKSKSQAGHKSMLERAAAAAATSHQPASEESQAEINLIG
                     CTAEEARDRADKFLDDAAMHQMSQVRIIHGMGMRILRREIENLLEGHPHVARFYKAPN
                     NQGGAGATIVELRQ"
     gene            complement(4357..>4736)
                     /locus_tag="PKZ53_03440"
     CDS             complement(4357..>4736)
                     /locus_tag="PKZ53_03440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003395623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ39518.1"
                     /translation="FAKLAAQFSLDTGSAKEGGDLGYFGRGQMVKAFEDAAFTLKPGE
                     ITPSPVITEFGVHIIKVTDHRTRPLDKPLQDQIRDMLKGKQLEERLDAMVRASQISVA
                     ADFEIPEANPQQLPGRSAPPGHP"
BASE COUNT         1056 a         1154 c         1343 g         1183 t
ORIGIN      
        1 ggtcgaagtt gatgaagcgg gtggattccc agcggaggtg gtgcccgtgg gctgattggg
       61 ttgtgcgggt tggttgggtt ggttgggctg acaacccaga cttgaaacgc agacaacaaa
      121 caaacatccc aagacgaaag aggaaactcg cttacctgag cgaagagaga aaaaacgcat
      181 cataagtcaa ataaattgtt caatattgag tgacctgaag ccccactgat tgatgtgatg
      241 agcaactgaa tcaggttgtc tttggtccga gaaactggcc ctgtgaaact gatagtgccg
      301 ggaaaaaaat ttgattttga aagaatggac ccacaccctg gttgaacaca agaggaattt
      361 cgtgcaagtt cgtgtggtgg ctgatgagca gtggtatcag gttgaatact ggtttcgcca
      421 gggatgtcac tgtcaggatt tcggttccaa cacctggtcc aggcagttta cctgaacctg
      481 gtatttggtc agatcctgat ccgggtacat gcgaaccacg gtgccggatt cgacatgaaa
      541 acaactcaat gggtcttcaa aagtgcatcc agtatcaagc ccgatggcgc tgccgcacac
      601 atagatttca ccgggttggc gaaccccatc ttgcggaagg taccgggtcg gtgtatggcc
      661 gaaaaaacag ggctttccac gatacagttt aaaaaacttg agatcccggg tccaaatcaa
      721 gacatctggc tcgcattccg acgggtgttt ccccggaaca atcccggcat gcacatacag
      781 ggctttatca tcttcataga agaagggcat gcggcggaag aagtcgaggt gttcttgcgg
      841 aatatggagg gaaatggttg atgtttcttc gttgatttcg cacccatact gattcatggt
      901 cataatgccg ccgttttcgg ggatgagcca gagtgggtct ccctcgtcca gtaaatcgag
      961 cagcatttgc tcatgattcc cccgcaacgc cacaacccgt ggattctgtg cctgcagttc
     1021 aatcacatag gagacaaccc cgggcgcatc ctcgcctcga tcaatcaggt ctccgacaaa
     1081 aatgatccgg tcccgccgag gattgtaatc gagcgattca acaattcttc gtagttgccg
     1141 ataccttccg tgaatatcac cgaatacgta ggtgttttgc attccgggta agcctccggg
     1201 tgaacaatct ctttgaagtc tgggtgcgca gaaaacagaa tcacagcaaa gctctcacgg
     1261 aaagcgaagc ggcaggatga tcgcttccag aaagcgtcag ttgagatctt gaactggggg
     1321 tgtataggct gaattcagga tacctagtct agcagaagtt tgcgccaaga ctctagataa
     1381 aaatggcaga tatggagtac ggcggcttga cgccgctttt cataaagact ttggagtgcg
     1441 atagcttgat gctgctttgg gattcgatgt gaaagcggcg tcaagccgcc gcactccaaa
     1501 acatttattg tcggagttcc acaattgtgg caccggcacc gccctggttg tttggggctt
     1561 tgtaaaaccg tgctacgtgg ggatgtcctt cgagcaggtt ttcaatttcc cggcgtaaaa
     1621 ttctcattcc catcccgtga atgatgcgta cctggctcat ctggtgcatg gccgcatcat
     1681 ccaggaattt gtcagctcga tccctggctt cttcagcggt gcagccaatc aggttgattt
     1741 ccgcctggga ttcttctgaa gctggctggt gactggtggc cgcggcggcg gcggcccgct
     1801 ccagcattga tttatggccg gcctgcgatt ttgatttgct gattcgtcgg acgtgggtgc
     1861 ttgccgtctt taaccgcatg gatccaaccc gaaccagcag catgccctgc tgaatggatt
     1921 caatgatgcc ctcctggcca agatcggttc ggaccaggtc gccgacccgc agttcgcctt
     1981 cttcaacgcc tggttcttcg gttctggccg gttcaggaag ttgaggcatc gtcggaggtg
     2041 gcgttggttt gaccagccgg agcgcgtttt gttgtttgaa tttttcattg gcggcccggg
     2101 ccagtttttc aactgcccgt tcggctgaat gttgggcttt ggaacgttga acctgatctg
     2161 agatctgggt caacaggggg cgaacctgct caaaaaattc atcccgaagt tcattgagtg
     2221 tttcggtaaa cactttttgg cgtgccagtt cacgttcagc aaactggtgg tcaagccggg
     2281 catagcgttc agccagcgtt gcccgttctt cttcaagtgc cacgtttgaa tcgtggtgat
     2341 agtcaatttc cgatttgagt ttttcaaggt agtgggacag gtcacgttca cgagaaccca
     2401 gtttctgcgt tgccagatcc agaattgccg ggctgattcc cagtcgccgg gcgatttcaa
     2461 ttccgcttga ggccccagcc tgggtcgtca gcaaccggta ggttggttgc agggtttcaa
     2521 aatcaaactc aaccgaagca ttaaataccc catcggtggt gtcggcatac acttttaatg
     2581 ggttgtagtg ggttgaaaca atcgtgtgga tggtctgtga gcggagaaaa tcaacgatgg
     2641 ccgttcccag tgccgcgcct tcatccgggt ctgtgccggt cccaatttca tcaatcagca
     2701 ccagggccgg ggccgctacg gtttctgtga tggtgcggat attggtgatg tgcgacgaga
     2761 aggtggacag gtttgccgcc agtgactggt ggtctccaat atccgcatgg atttgatgga
     2821 acaggcagat attggcttcg cgggcgggaa cgtgcaatcc actttgggcc atcagcgcac
     2881 agattccagc cgttttcagc accacggttt tgccgccagc attcggaccg ctgatgatca
     2941 tgacctggtc agcactgttg agtgtaaagc tgatagggac gatctttccg ccttccgtga
     3001 ggagtcgcga ttcgagcagg gggtgtcggg cgtcaatgac tgaaaacacc ccatcggtgt
     3061 tgagtttggg ttcaaccgca ttgaaggtct cggcaaaccg ggctttggcc gccagaaagt
     3121 caatttctgc cagcgtccag gtcagttgtt caattgcttt gtgttcggtg cgcagggctt
     3181 cggtaatctg aaaaagaatc cgggcaatct cggcttcttc catatcctga aggcggacca
     3241 tttcattgtt gagttcaatt gtcccaattg gttcaataaa aacagtcgcc ccgcttgatg
     3301 aggctgcgtg aaccacgccg gggaggtggc cccggtgatc gctccgaatt ggcagcacaa
     3361 atctcccgtt gcgctgagtg atgatttgat cagaaaacgc cggttccaat tcggcctggc
     3421 ggagcattcc ttcaagctgg cgatggatgc gggcacgctg gtgttggatt tcctgccgga
     3481 tgcgccgtaa ttcagagctg gcccgttcgt caatttcacc cgtcggaagt agaatccggc
     3541 gggtcgtttg atagagttta ctcaggttgg gcaacaggtg aaaaacactg ttgagggtcg
     3601 ggaactcatc ccggactgga cgaaacattt cctggagtga aataccaacc tgcataaagc
     3661 gcaaaacggt ttgcaattga tcggtttcca ggcgggtgcc ggcaatgcgt agttgttcca
     3721 gaattgatgt tggatcgggt aaatctccga ctgagagtcg cccaacttcc tggctaaacc
     3781 ggcgagcttc agtggttaga gtcaggttgc gagtgacggt gggccactca tcggtcggtg
     3841 tcagctccag cgccagcagg cgtccctgtt cagatttggc ctcgcgggcg agcatgactc
     3901 ggagatgtgg aaattcaagt gtctcaagag cttgtattgg catggttttt ctttgaaaga
     3961 aattgttccc caggttgaac cctcattgga ggctcagttg aagtgagata ccgagacctc
     4021 acctcgccgc cccggttttt tgagcttcac tccttctgac gctatcgctc gtcaggtgat
     4081 gggtttggcg acccacccgg tggctcagac atgaaggcgt cactgttgat gcagattgtt
     4141 catggggaaa tctcggataa caccctcgaa gcagcagaat cgtctggcaa ttcggcgagg
     4201 agttgcgtga attgagagag aggacaactg ggttggcact gacacccggc aggttctgtg
     4261 ctgccgggtg tcgggtggcc tctctggttg acaacgaaaa ccctggtcaa aaagaggaaa
     4321 aggtgagccc tggttcacgt ttctgctgca aaccgattat ggatgtcctg gaggggcgct
     4381 gcggccaggc aactgctggg gattggcttc tggaatttca aagtcggcag ccactgaaat
     4441 ctgacttgcc cggaccatgg catccagtcg ttcctccaac tgttttcctt tgagcatatc
     4501 acggatctga tcctgcaatg gtttgtccag aggtcgggtg cgatgatcag tgactttaat
     4561 gatgtgcacc ccaaactcgg taatcaccgg tgagggcgtg atttcgccgg gctttaaggt
     4621 aaacgcggca tcttcaaagg ctttaaccat ttgaccccgg ccaaaatacc caagatcccc
     4681 accttctttc gctgaacccg tatccaatga aaattgcgcg gccagtttgg caaaat
//
LOCUS       DAOQDO010000145         4726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_11988_length_4726_cov_2.741383, whole genome shotgun sequence.
ACCESSION   DAOQDO010000145 DAOQDO010000000
VERSION     DAOQDO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11988_length_4726_cov_2.741383"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1676
                     /locus_tag="PKZ53_03445"
     CDS             111..1676
                     /locus_tag="PKZ53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39519.1"
                     /translation="MLTSNTLLQNRYRIIRSLGEGGMGAVYEAKDERLDSIVALKETF
                     FSQEELRKAFEREAKLLARLHHRALPVVSDHFSDDQGQFLVMQFIPGKNLEQLLALNG
                     GSFQMEQVLRWADGLLDALEYLHTQEPPVIHRDIKPANIKLTEAGNIFLLDFGLAKGH
                     RSLSEMTTGAGSIMAFTLSYAPLEQIQGTGTDPQSDLFAFGATIYHLLTGKAPVDALT
                     RAMTIVGENPDPLQLASEINPMVPLPIAEILHQALSLKAANRPASARDLRRHLRLAAQ
                     SLNLYNFPYDDRPQPASMRLFVPSSSTGGQEVSPVAVTKAGNKTAEGLSTAETVFDSL
                     APLARQSDGLTSARPTGAAAVPVQPTAPPVSSNTRSVTRWWKNVPRSWLIGIGVLVVA
                     GGLGLRMMGPPPPRPALPPGATEFHRSGQATVTDPSTIVQKSELEMAGKVLRRLESDY
                     PLGARWFGRKGDVVVDVLISEDGLVEMARPLSGPPHLYQPAVNAAAEWEFQPTLQNGK
                     PVKVSTTITFQYR"
     gene            1848..2714
                     /gene="galU"
                     /locus_tag="PKZ53_03450"
     CDS             1848..2714
                     /gene="galU"
                     /locus_tag="PKZ53_03450"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNJ39520.1"
                     /translation="MKRVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPLIQYGVEE
                     ALASGIEQIIIVTGRGKNVLEDHFDISFELETTLRDRNKVALLEEVQSVSRMVNVAYV
                     RQKEALGLGHAVLVAKELIGDEPFAVILSDDIIDSQTPCLKQLVSAYERYGHSIISTT
                     RVEGPAISRFGVLDVEAVSERTYRIRDMVEKPPADQAPSNLAIIGRYILTPEIFPALE
                     RTPRGSGGEIQLTDAMRLLLKEQPMYAYEFEGTRHDAGDKLGFLIATVEYALKRPDLG
                     PEFREYLKQMKF"
     gene            2880..3812
                     /locus_tag="PKZ53_03455"
     CDS             2880..3812
                     /locus_tag="PKZ53_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ39521.1"
                     /translation="MNRILVTGGAGFIGTHLCRRLLDDGHEVICADNFYTGNKRNVQS
                     LLSHPNFELIRHDIVDPLLVEVDQIYNLACPASPVHYQANPIKTIKTNVLGVLNMLGL
                     AKRVKARILQASTSEVYGDPLEHPQREDYWGNVNPIGPRSNYDEGKRVAETLMMDYHR
                     QNGVDIRIIRIFNTYGPYMHENDGRVVSNFICQALRGNDLTMYGEGNQTRSFCYVSDL
                     VEGMVRMMNQPDFIGPVNLGNPGEFTIRELADKVLAMTGSASKIALRPLPVNDPQRRR
                     PDISLAKEKLDWSPAIPLDEGLAHTIAYFRSRNI"
     gene            3881..4660
                     /locus_tag="PKZ53_03460"
     CDS             3881..4660
                     /locus_tag="PKZ53_03460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ39522.1"
                     /translation="MDSFQGKTVLLTGASVGIGKCFAEQLAEKKANLILVARREDKMR
                     VLADELTRRHGIKVDVIAKDLSHPSAPKELFEETTRRGLQVDVLINNAGFGIGGAFLD
                     ATLERNLEMIQLNITTLTELTYRYLPGMVERKSGAVINVASTASFQAVPYLGTYSATK
                     AFVLSFSEALWEEYRHHNVTVMALCPGTTATEFFDVAGVKERTAMQTPDDVVRTALRG
                     LKAKRSHIISGMTNYLMAQSNRLVPREIGTRIAGSLFKPEK"
BASE COUNT         1135 a         1231 c         1231 g         1129 t
ORIGIN      
        1 cctttggtaa ctggtgctta gtccctggtc cctggtactg agaaaccagg cactttcaga
       61 aacaaagcac caaaaacaaa gaactaagta ccagttaccc gtcaccgatt atgctcacct
      121 ccaacacgct gctccaaaac cggtatcgca tcatccgttc acttggtgag ggtgggatgg
      181 gtgcggtgta tgaagcgaaa gatgagcggc tggattcgat tgtcgcactc aaagaaacat
      241 tcttttctca ggaagaattg cgaaaggcct ttgaacgcga agccaaattg ctggctcgac
      301 tccatcaccg ggcgcttcca gtggtcagtg accatttctc agatgaccag gggcagttcc
      361 tggtcatgca atttattcct ggaaagaacc ttgagcagct cctggccctc aatggtggat
      421 cgtttcagat ggaacaggtt ttgcgctggg cagatggatt attggatgcc ctggaatatc
      481 tgcacaccca ggagccgcca gtcattcatc gggatatcaa acccgccaac atcaaattga
      541 ccgaggctgg aaatatcttt ctcctcgatt ttggtctggc aaaaggccat cgctcgctct
      601 cagagatgac cacgggggct gggagcatta tggcctttac actgtcatat gccccacttg
      661 aacaaatcca gggaactgga actgacccgc aaagcgattt atttgctttt ggagcaacaa
      721 tttaccattt gttgactgga aaagcgcccg tggatgcctt aacccgggcg atgaccatcg
      781 tcggggaaaa tccagatcca cttcaacttg cgagtgaaat caacccaatg gtgccactgc
      841 ccattgcgga aattctgcac caggccctgt cactgaaagc tgcgaaccga ccggcttcag
      901 ccagagacct gcggcgtcac cttcgattgg cagcccaaag tctcaacctg tacaacttcc
      961 cgtatgatga ccgcccccaa ccggcttcga tgaggctgtt tgttccgtca tcttcgaccg
     1021 ggggccagga agtttcaccc gtggcagtga ccaaagcggg aaataagacg gctgagggtc
     1081 tctcaacggc tgagactgtg tttgattccc tggcgccact tgcccgtcaa tcagatggat
     1141 taacttctgc ccgtccaact ggcgccgccg ctgttccggt tcaaccgacc gcaccacctg
     1201 tttcatctaa caccaggtcc gtgactcgat ggtggaagaa cgtgccccga tcctggttga
     1261 tcgggatcgg cgtgctggtt gtggctggtg ggttggggct gcggatgatg ggcccaccgc
     1321 ctccgcgtcc ggctttaccg cctggggcaa ctgaatttca tcgttcaggt caggcaactg
     1381 tcactgatcc gtcaacgatt gttcaaaaat ccgaactgga aatggctggg aaagttcttc
     1441 gccggcttga gtcagattac cctctgggcg ctcgatggtt tggacgaaaa ggagatgtgg
     1501 tggttgacgt cttgatttca gaagacggac tggttgaaat ggccagacct ctcagcggcc
     1561 caccacatct gtatcaacca gcagtgaatg ccgctgctga gtgggaattt caacccacgc
     1621 tccagaatgg caaaccggtc aaagtctcga ccaccattac cttccaatac cgatagctcc
     1681 gtctggttgg tgcgctcgct acattgtttt cgcctgtggt acgtcgcttg ctctggtgaa
     1741 actgtgagcc tggaatttac ctcagcctga gccccatacc ctcagcccaa gatggtatca
     1801 cacctctcat caccattttt cagactgtac caaaggagca ctcacctatg aagcgcgttc
     1861 gcaaagctgt ttttcccgcg gctggacttg gcacccggtt tctccccgcc accaaagccc
     1921 aacccaaaga aatgttgccg ctcgttgata aaccgttgat tcaatatggg gttgaagaag
     1981 ccctggccag cggcatcgaa caaatcatca ttgtcaccgg gcgcgggaaa aacgtgctcg
     2041 aagatcactt tgacatttca tttgaactgg aaaccacgct tcgtgatcga aacaaagtgg
     2101 cgcttctgga agaagttcag tcggtctcgc ggatggttaa tgttgcctat gttcgtcaaa
     2161 aagaagcact tggccttggt catgcggtgc tggttgccaa ggagttgatc ggagatgaac
     2221 cctttgccgt cattttgagc gacgatatca ttgattccca aaccccgtgc cttaaacaac
     2281 tggtctcggc ctatgaacgg tatggtcatt caatcatcag taccacccgt gtcgaaggcc
     2341 cagccatttc acgatttggg gtactggatg ttgaggcggt aagtgagcga acttatcgaa
     2401 tccgtgatat ggttgagaaa ccaccggctg accaggcccc ctcgaatctg gctatcattg
     2461 gccgctacat tttgaccccc gaaatattcc cagcccttga acggacgccg cgtggctccg
     2521 gcggtgaaat tcaactcacc gacgctatgc gtcttctgct caaggaacag ccgatgtatg
     2581 cctatgaatt tgaaggaact cgccacgatg ccggcgataa acttggattt ttgattgcca
     2641 cagtcgaata tgccctcaag cgacctgacc tggggccgga gtttcgcgaa tatttgaagc
     2701 agatgaaatt ctgaataaag gcgggttccg ggttgaaaaa acagggcttg gggctgaggg
     2761 ctaaaagaac aagttcatga ggttgccttc agatttcttt tcgtcctttt tgttcctttt
     2821 gtccttttcg tccttttgtt tttttaaaac cctgaaccct gaaccctgaa ccctgaccta
     2881 tgaaccgaat tctcgtcacc ggcggcgccg gttttattgg aacgcatctc tgtcggcgat
     2941 tacttgatga cggacacgaa gtcatttgcg ccgacaattt ttataccggc aacaaacgca
     3001 atgtgcagtc gcttctgagt catccgaact tcgagttgat ccggcatgac attgttgacc
     3061 cgttgctggt cgaagttgat caaatttaca acctcgcctg cccggcttct ccggttcact
     3121 atcaggccaa cccaatcaag acgatcaaga caaatgtgct tggggttttg aatatgctgg
     3181 gactggcgaa acgagtgaaa gcgcggattt tgcaggcgtc aacgtcagaa gtgtacggtg
     3241 acccgttgga acatccccaa cgcgaagatt actggggaaa cgtcaatccg attgggccgc
     3301 gcagcaacta tgacgaaggt aagcgtgtgg ccgagacgtt gatgatggat tatcaccgcc
     3361 agaatggtgt ggacatccgg atcatccgta ttttcaacac ttatggtcct tacatgcacg
     3421 aaaatgacgg tcgggtggtg agtaacttca tttgtcaggc gcttcgtggc aatgacttga
     3481 ccatgtatgg tgaaggcaat caaacccgtt cattttgtta tgtttccgat ctggttgaag
     3541 gcatggtgcg gatgatgaat cagccggact tcatcggacc ggtcaatctg ggcaatccgg
     3601 gcgaattcac catccgcgag ctggctgaca aagtcctggc gatgacaggc tccgcttcaa
     3661 aaatcgcctt gcggccattg ccagtcaatg atccacaacg gaggcgcccg gatatttcac
     3721 tcgccaaaga aaagcttgac tggtcgccgg ccattccgct tgatgaaggg ttggcgcaca
     3781 cgattgcata ttttcgatcc agaaacatat gaaagcggtg tcaagccacc gcactccaaa
     3841 agcggcgtca agtgggccgc actccaaaga gggaaaatct atggattcct ttcaaggaaa
     3901 gaccgtgttg ttgacaggcg cttcagttgg tattgggaaa tgttttgccg aacaactggc
     3961 tgagaaaaaa gccaatttga ttctggttgc gcgccgggaa gataaaatgc gcgtcctggc
     4021 tgatgaattg accaggcgcc acggtatcaa agtggacgtc atcgccaaag atttatcaca
     4081 cccatcggcg cccaaagaac tttttgaaga gaccacccgg cgcggtttgc aagtggatgt
     4141 cctcatcaac aatgccggat ttggaatcgg cggagctttt ttggatgcca cgctcgaacg
     4201 caatctggaa atgatccagc tcaatatcac aacgttgact gagttgacct accgctattt
     4261 gcctggaatg gtcgagcgca aaagcggtgc ggtgatcaac gtggcttcga cagcttcttt
     4321 ccaggccgta ccatacctcg gcacctattc ggccaccaaa gcctttgtct tgagtttttc
     4381 cgaagcgttg tgggaagaat accgccatca caacgtgacg gtgatggcgc tgtgtccagg
     4441 cacaaccgcg actgagtttt ttgatgtggc tggggtgaaa gaacgcactg ccatgcaaac
     4501 gccggatgat gtcgtccgaa ctgccctgcg tggcttaaaa gccaaacgca gccacattat
     4561 ttcggggatg accaactatt tgatggctca gtcaaaccgg ctcgtcccac gcgaaatcgg
     4621 cacccgcatc gctggaagtt tgtttaaacc agagaagtag ggcggaagac atcgggctaa
     4681 gggctaaggg ctgaagaaat tgggctgaag actcgcaagc tcgggg
//
LOCUS       DAOQDO010000146         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12011_length_4721_cov_2.965709, whole genome shotgun sequence.
ACCESSION   DAOQDO010000146 DAOQDO010000000
VERSION     DAOQDO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12011_length_4721_cov_2.965709"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKZ53_03465"
     CDS             <1..835
                     /locus_tag="PKZ53_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNJ39523.1"
                     /translation="PPPPDAKYKKNNHASAWHLDYKNDLRSLMSVEPNAEWYETTHHE
                     LGHIYYYVSYTNPDVPPLLRNGANRAYHEAIGSMIGLASMQKPFLEGRGLAPKDVKVD
                     QTQALLKEALNYVVFIPFSAGVMPGFEYDVYEKNLPKDKLNQRWWELAAKYQGITPPS
                     KRGEVYCDAATKTHINDDAAQYYDYALSYVLLMQLHDHIARNILKQDPHATNYYGSKE
                     VGDFLRKIMTPGSSRDWRVVLREATGEDLSAKAMLRYFEPLMEYLKKENKGRKHTLPE
                     L"
     gene            complement(998..2962)
                     /locus_tag="PKZ53_03470"
     CDS             complement(998..2962)
                     /locus_tag="PKZ53_03470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ39524.1"
                     /translation="MRYCPICKRCYEDTELRCAQDGLFLVEAFPGPRAVDGKYRIDML
                     LGQGGMGSVFKATHLELDRVIALKIVLPDFVSSNETLERFRQEARAAARLNHPNVIGV
                     YDFGVLPTGQAYLAMELLRGRSLRDEIEDLGQLTPQRVLEIMRPVCEAVHAAHCAGVI
                     HRDLKPDNIVVEASDGGLEQVKVVDFGIAKLKERSGIHSLNLTEPGLVMGTPHYMSPE
                     QCRGEELDARSDVYSLGVTVYEMLSGRVPFDAPTPSAVIIQHAIDPPRRLRLLRSDIS
                     EVLENVVMRSLSKNRELRQQSAMELYTDLSQAVNPPVTVEKSASFAPPKSKPITGQFT
                     RFEPEDTAIPSISPLFTTGKSHVAQVIKLSEKSALQGHEHVIKSLDFSPDGTSLLSAS
                     GDGTIRLWDMTTNQERSVLRGHEYSVNCAIFSPDGTRIASAGADGTVRLWLVKGEREI
                     IALGHKASVRSLSFAPDGRWLASACDTEIKLWDTIRYRQITSLEGHTKTVESVAFSPD
                     GNFLASGSADDTVRLWDMVTKTEQESLLRAGHAVTAVAYSPQGHLLASAGRDQVVCLW
                     DLNNLPEGCKVLWGHDEAVRAIAFSADGLWMASGDWNGELRIWNLENQEIIASEEAHD
                     GSIFALRFSPKSNLLVSGGYDTTIKVWQVG"
     gene            3185..4675
                     /locus_tag="PKZ53_03475"
     CDS             3185..4675
                     /locus_tag="PKZ53_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39525.1"
                     /translation="MSNLPPHSALAAAIEQLRSGNGAQAEALIVNATEAIFTQYGPES
                     PEYAAALFDLGSFFVAIESFERAVDTMREATKVPYLNESGQRDHLTYVLNLGELLIHV
                     EQFEEAEAVLRTNLTDREAFYGRQHAGYAFALIPLAEVIWLTGNQDEAQELILQAVQI
                     LWVEGNPRVAQAIALQAFILASNGFDSGFELIRDVPLELIEDVLQQVFRRQDVADPGL
                     VLMVLQDALRWVDSLSETPLEFKQQILAQLSTVARTAGEHSVRLGALKALLEVSQKAG
                     DFRQEIDVEMALGLALSEMNEPEKADYAYQRAGELARSLNDPGLYAGVLRNYGLFLAD
                     LDRRADAEPVLRKSVAIAQGGLDQLQTGRALIALGIFLQHDHKLAEAQELLEQALGIL
                     PVAHPDTLYARSHLDAIQTNQSCGCGDMSDAIAVTLKALVEPNLPPDLLDEISLGEDM
                     DVQVQLKREPTEEEVDQLNRVIGHAVRELRERLRRQGFSGTERRAE"
BASE COUNT         1175 a         1176 c         1257 g         1113 t
ORIGIN      
        1 cccgcctccg cccgatgcca aatacaagaa aaacaaccac gccagtgcct ggcacctgga
       61 ttacaaaaac gatctgcggt ccttgatgag cgtcgagcca aacgctgaat ggtatgaaac
      121 cactcatcat gagttggggc acatttacta ttatgtcagc tataccaacc cagatgttcc
      181 gcctttgctc cgcaatggtg ccaatcgggc gtatcacgaa gccattggca gcatgattgg
      241 gctggcgtca atgcagaaac ctttcctgga aggtcgtggt ctggcgccaa aagatgtcaa
      301 agttgatcaa acccaggcgt tacttaaaga agcgctcaac tacgtggttt tcattccatt
      361 ttctgccgga gtgatgcctg gatttgaata tgatgtgtat gaaaagaacc tgcccaaaga
      421 caaactgaac cagcgctggt gggaattagc tgcaaagtat caagggatta cacctccttc
      481 aaaacgaggt gaagtctact gtgacgctgc taccaaaacc catatcaatg acgatgcggc
      541 tcagtattat gactatgctt tgtcctatgt gctcttgatg cagcttcatg atcatattgc
      601 ccgcaacatc ctgaagcagg atccacatgc gacgaactat tatggcagca aagaagttgg
      661 cgattttctg cgcaaaatta tgacgcccgg cagttcacgt gactggcgtg tggtcctgcg
      721 cgaagccacc ggcgaagacc tgagtgctaa agccatgctg cgctattttg aacccttgat
      781 ggagtattta aagaaggaaa acaaggggcg gaaacatacg ctgccagagc tgtaaggtgg
      841 ggctgaagaa agtgggttga agaagtcggg ctgaagaaag tgggttgaag aagttgggct
      901 gaagaagtcg ggctgaagaa agtgggttga agaaaatggg tttaataccc tgaacccgac
      961 ttcttcagcc ccaagcccaa tgtcttccgc cctggcttta cccaacttgc cagactttga
     1021 tcgttgtatc atagccgcct gaaaccagga gattgctttt gggggaaaag cgcagggcaa
     1081 agattgaccc gtcatgagct tcttcagagg caataatttc ctggttttcc aggttccaga
     1141 ttcgtagctc gccattccaa tcaccagaag ccatccacaa cccatcggct gaaaaggcaa
     1201 tggccctcac tgcttcatcg tgtccccaga ggactttgca gccttcaggt aagttgttga
     1261 gatcccaaag gcaaaccacc tggtcccgtc ctgctgaagc cagcagatgc ccctgtggtg
     1321 agtaggccac tgccgttacg gcatggcctg cccgcaagag cgattcctgc tcagttttgg
     1381 tcaccatatc ccacaagcga accgtatcat cggcactccc cgaggccaga aaattcccat
     1441 ctggcgaaaa ggcaactgat tccaccgttt tggtgtgacc ttcaagcgat gtaatttgcc
     1501 gatagcgaat ggtatcccac aatttgattt ccgtgtcaca agccgaagcc agccagcgtc
     1561 catccggcgc aaagctcaac gaacgcaccg aagctttatg acccagagca atgatttcgc
     1621 gttcgccttt aaccagccac agtcggactg ttccatcagc gccggctgag gcaatgcggg
     1681 tgccgtccgg tgaaaaaatg gcgcagttga ctgagtattc gtgtccgcgc agcaccgaac
     1741 gttcctggtt ggtggtcata tcccataacc ggatcgtacc gtctccgctt gcagagagca
     1801 gggaggtgcc atcaggggaa aaatccaacg atttgatcac gtgttcgtga ccttgaagcg
     1861 ctgatttttc cgagagttta atgacctgag cgacgtggga cttgcccgtg gtaaacaatg
     1921 gcgaaataga tggaattgcc gtgtcttccg gctcaaaccg ggtaaattga cctgtaatgg
     1981 gtttggattt cggaggcgca aaactggcgg atttctcgac ggtaaccggc gggttcacgg
     2041 cctgagatag gtcagtgtag agttccatcg ctgattgctg gcggagttcg cgattttttg
     2101 aaagcgagcg catgacgaca ttttcaagaa cttcactgat gtcgcttcgg agcaatcgca
     2161 gccggcgtgg cgggtcaatc gcgtgctgaa taatgaccgc cgaaggggtt ggtgcgtcaa
     2221 atggcacgcg accagagagc atttcataca ccgtcacacc caggctatag acatcagacc
     2281 gggcatcgag ttcttcaccc cggcactgtt ccggcgacat atagtgcggc gttcccatga
     2341 ccaatccagg ttcggtcagg ttcagggagt gaataccaga tcgctctttg agtttggcaa
     2401 ttccaaaatc aaccacttta acttgctcca gcccgccgtc actggcttcg accacaatat
     2461 tgtcaggttt taaatcgcgg tgaatgacgc ccgcacagtg tgccgcgtga acggcttcac
     2521 acactggacg cataatttcc aggacacgtt gaggtgtcaa ctggcctaga tcctcaattt
     2581 catcacgcag tgaccgacca cgcagcagtt ccatggccag atacgcctgg ccagttggca
     2641 acacaccaaa gtcatacacg ccaatgacat ttgggtggtt gagtcgggca gcggcgcgtg
     2701 cttcctggcg aaagcgttcg agggtttcat tgctggatac aaaatcaggc agcacgattt
     2761 tgagggcgat aactcgatca agttcaagat gggttgcttt aaagacggat cccatcccac
     2821 cttgacccag gagcatgtca attcggtatt tgccatcaac tgcgcgtgga cccggaaatg
     2881 cttcgactaa aaagagtcca tcctgggcac aacgcagttc agtatcttca taacatcgct
     2941 tacagattgg gcagtatctc atgagcgtga aggctctcgg ctaacgctgt caggccggtg
     3001 cggctgacac tggcttgcag tgggattatg ggaaatagac ttcaggattg aaataaagag
     3061 atgattcgtt tcagaatgcc cgcaagtgac gccgcgagcc cggccagtgt agcacatctt
     3121 tttttccgct gagtaggttt cggcctcaaa ttgccttgaa tcatttcttt accaggagaa
     3181 tcgaatgtca aatcttccac ctcacagtgc gctggctgcg gccatcgagc aacttcggtc
     3241 aggcaatggc gcccaggcag aggcactgat tgtcaatgcg accgaagcga tcttcaccca
     3301 gtatggacct gagagtcccg aatatgctgc ggccctgttt gacctgggga gtttttttgt
     3361 ggctattgaa agttttgaac gggcggttga caccatgcgt gaagccacca aagttcctta
     3421 tctgaatgaa tccgggcaac gtgaccatct gacctatgtg ttgaatctgg gtgagttatt
     3481 gattcacgtt gaacaatttg aagaagccga ggcggttttg agaaccaact tgactgatcg
     3541 tgaagccttt tacggtcgtc aacacgctgg atatgccttt gccctgattc ctctggctga
     3601 agtcatctgg ttgactggaa atcaggatga agcccaggaa ctcatcctgc aagccgtgca
     3661 aattctttgg gttgagggca acccacgagt ggctcaggcg attgctctgc aagcatttat
     3721 cctggccagc aacggatttg actcaggatt tgaactgatt cgcgacgttc cacttgagtt
     3781 gattgaggat gttttgcagc aggtctttcg ccgccaggat gtggccgatc ctggattggt
     3841 attgatggtt ctccaagacg ccctgcgctg ggttgattcg ctgtctgaga cgccacttga
     3901 gtttaaacaa cagatattag cgcaattatc aaccgtcgct cgaacggctg gcgaacattc
     3961 agttcggctt ggagcgctca aggcattgct ggaagtcagc cagaaggcag gtgattttcg
     4021 acaggaaatt gatgttgaaa tggcgttggg gctggctctc agtgaaatga atgaaccaga
     4081 aaaagccgat tatgcctatc aacgggctgg tgaactggca cgctcattga atgatcctgg
     4141 attgtatgcc ggagtcttac gcaactatgg attgtttctg gcagatctgg atcgccgtgc
     4201 cgatgctgaa ccggtgctgc gtaaatcagt tgccattgcg caagggggac tcgatcagct
     4261 ccagaccgga cgcgccctga ttgccctggg cattttcctc caacacgacc ataaactggc
     4321 tgaggcccag gaactgttgg aacaggccct tggaattttg ccggttgcgc accctgacac
     4381 cctctatgct cgaagccatc tggatgcgat tcaaaccaat caaagctgtg gatgtggcga
     4441 tatgtctgac gcaattgccg tcactttaaa agcgctggtg gaacccaatc tgccacctga
     4501 tttgctggat gagatttcgc tgggtgaaga tatggacgtg caggtccagc tcaaacgtga
     4561 accaaccgaa gaagaagttg atcaactgaa tcgggtaatt ggccatgccg ttcgcgaact
     4621 ccgcgaacgc ctccgccgcc aggggttttc aggcactgaa cgcagagctg aatgaagaat
     4681 gaagaatgaa gaatgaagaa tgaagaatga agaatgaaga a
//
LOCUS       DAOQDO010000147         4698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12108_length_4698_cov_2.732931, whole genome shotgun sequence.
ACCESSION   DAOQDO010000147 DAOQDO010000000
VERSION     DAOQDO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12108_length_4698_cov_2.732931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="PKZ53_03480"
     CDS             complement(<1..2323)
                     /locus_tag="PKZ53_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNJ39526.1"
                     /translation="MQRLWVSSIVGCGVLISSLLISHWGNVGFLKPVSANPLWTTLAS
                     ATGSSLSQATANNQRVFQLNSSLIEQTLNRAPKEGQLPIRQSQAILQFPLPDGTFLSA
                     RIAESPIVEPPSPDIQIRTFTGQGIEDPTATLRFDWSPDGLHALLLTSQGSVFVRPAG
                     DPNHPGTYVSSFGTDADWQPGQFQCGAMPQEVVDQKFPRIQEYQLESDGLASGGVFNS
                     SPDPTSSTEAPGLRQAAEDRRYAAQSIPDEMATRLQTFSNTAYLLKTAQFTANNGWMI
                     VYGYSGFFARNIPQAAFDRALSLNNSGNELFGVYFSPSNGWVILYGENQFAAQGIPTA
                     MQNRLQTLANENRPIKYIAFPPTGGWVILSDYNQFFSDGIPEDARTALTELAGQAEEL
                     KSIAFTSNNQWVIVYGNARFRASSASLSSSLQAKLAQFRQENRNFRSATLTADGNGWV
                     ILAAAPPLPVGNTLRTYRLAVSATGEYTQRFGNGNVTRTFESIVQRVNFANAIYEREM
                     SVRFLVVNDQDAIFTSGSSDPFTNGNPREMDFENVDVLNGKVGATAYDLGHAFGTVGE
                     GGTFGTAFFGLCDPNFKALGATCFGVAEDTPLSTYTLVHEMGHQQFANHTFNGSTRGC
                     GNGNRQGVAAVEPASGTTIMGYGPTGCGEESLGFKPEDIYFHAFSLTQMLNAATEATP
                     GSCAAPISTGNQPPQVTVGPDLAIPAKTPFALTATATDPEGDPLTYCWEEVDVSFAQS
                     PPNSDEDGRIRPLFRSFPPTTSPMRTFPQVTNIL"
     gene            2522..3055
                     /locus_tag="PKZ53_03485"
     CDS             2522..3055
                     /locus_tag="PKZ53_03485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNJ39527.1"
                     /translation="MEFQSGQSLLIRPEQPGDAAGVRRVNELAFNQLDEANLVEALHK
                     VAVGLISLVAVLDDQIVGHILFSPATIQGAGGEFHAIGLAPMAVLPEYQNRGIGSQLV
                     RAGLAECRRQGHRVAIVLGHPKFYPRFGFVPARPRGITCEYDVADDTFMVAELEPGAL
                     AGCTGLAKYHPAFAMVG"
     gene            complement(3149..>4698)
                     /locus_tag="PKZ53_03490"
     CDS             complement(3149..>4698)
                     /locus_tag="PKZ53_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39528.1"
                     /translation="ASDLVSEQLHPVTTTAPAQVETILPNRLGWLSPRTAFLIGMVVI
                     IGMLGGFLMWPRPSLPQPHSSARSIAVLPFKVLQPQGNDEYLSIGLTDVLITRLSNLQ
                     QLVVRPTRSVLPFASSSKEAAEVGKELQVETVLDGSIQQMGDQVRVTVRLIQVSNSQT
                     VWAFQCDETCSDYFQLQDHISEQVTQALSYKLSDEEQRRLTKHYTENALAYQEYVKGR
                     YYTLLFTKDGLETAIRYFNHAIELDPRYALAYAGLADAYCTGAGTFLPSNEALPKAKA
                     MAEKALSLDTSLPEAYAALGHAKVHLWDRTAKQDLQKALELNPNLATTLMWFGEYYQT
                     FNPPESIPILIKARELDPVLSPVRGLLSFSYGLNGQFDLAHREIQEALTRHPTDSQLF
                     TLRALVYSYQKKYPQALAELDKIPPDVLMDFYRVYLLIRAGRKAEAQNLLARIVPHQK
                     ETPDSAHFMALMCVALNDKDQAFKWLEQAYQTHSVYLSYLQYDPELLPLRSDPRYDDL
                     IRRVGLS"
BASE COUNT         1091 a         1133 c         1332 g         1142 t
ORIGIN      
        1 gcaggatgtt ggttacctgt ggaaacgttc gcattggtga tgtggtcggt ggaaatgacc
       61 ggaagagtgg ccggatgcgt ccatcttcat cgctattggg cggactttgg gcgaaagaaa
      121 cgtcaacttc ttcccagcag taagtgagtg ggtcgccttc ggggtcagtc gcggttgccg
      181 tgagagcaaa tggtgttttg gcggggattg ccagatcggg tccaacggtt acttgcggtg
      241 gctggttccc ggtcgagatg ggtgccgcac agcttccagg cgtggcttca gtcgcggcat
      301 tgagcatctg ggtcaggcta aacgcgtgaa aataaatgtc ttcgggtttg aatccaaggc
      361 tttcctcgcc acacccggtt ggaccgtacc ccataatcgt agtgccgctg gctggttcaa
      421 ccgcagccac accctgccga tttccattgc cgcacccccg agtgctgcca ttgaacgtgt
      481 ggttggcaaa ctgctggtgg cccatttcgt gaaccagcgt gtaggttgaa agcggcgtgt
      541 cttccgcaac accaaagcag gtagcgccca gcgctttaaa attcggatca cacaggccaa
      601 aaaacgccgt tccaaaggtg ccgccttcgc caaccgttcc aaaggcatgc ccaaggtcat
      661 aggccgtcgc gccgactttt ccattcaaaa catccacgtt ctcaaaatcc atttcgcgag
      721 gattgccatt ggtgaatggg tcgctggagc cacttgtgaa aatggcatcc tgatcattga
      781 ccaccagaaa gcgaaccgac atttcgcgtt cataaatggc attggcaaag ttgacgcgct
      841 ggacgatgga ttcaaaagtt cgagtcacat tgccattgcc aaagcgctgg gtatattcgc
      901 cggtggccga gacggccagc cgatatgtgc gcagggtgtt tccaaccgga agtggtggcg
      961 cagcagccag aatcacccac ccgttgccgt ctgccgtcaa tgtggcgctc cgaaaattgc
     1021 ggttttcctg gcgaaactgg gcgagttttg cctgcagact ggatgaaagg gaagcggaac
     1081 tggctcgaaa gcgtgcatta ccatacacaa tcacccactg attatttgag gtgaaggcaa
     1141 tgcttttgag ttcttctgcc tgaccagcca gttcagtgag ggctgttcgg gcatcttccg
     1201 gaattccatc agaaaagaac tggttataat ccgaaagaat gacccatccg ccggttgggg
     1261 gaaatgcaat gtatttgatt ggccggtttt cattggcaag ggtctgaagg cgattttgca
     1321 tggcggtcgg aatgccctgg gccgcaaact ggttctcgcc gtagaggata acccacccat
     1381 ttgacggcga gaaatacacc ccaaagagtt catttccgct gttgttgagc gagagtgccc
     1441 ggtcaaaagc tgcctgcgga atgttgcggg caaaaaaccc gctgtaccca tagacgatca
     1501 tccagccatt gttggccgtg aactgggcgg tttttaacag gtaggccgtg ttggaaaagg
     1561 tttgaagccg ggtggccatt tcgtctggaa tactctgggc ggcataccgg cgatcttcag
     1621 cggcttgccg taaaccagga gcttcggttg agctggttgg gtcaggactg gagttaaaca
     1681 ctccaccgga ggcgagcccg tctgattcga gttgatattc ctgaattctg ggaaatttct
     1741 ggtcaacgac ttcctgtggc atcgctccgc actggaattg acccggttgc cagtcggcgt
     1801 ctgttccaaa tgagctgacg taggtccctg gatggtttgg gtcgccggct ggacgcacaa
     1861 acaccgaccc ctggctggtc aaaagcaggg cgtgcaggcc atcaggactc cagtcaaacc
     1921 ggagtgtggc ggttggatct tcgatgccct gacctgtgaa ggttcggatt tggatatctg
     1981 gtgaaggtgg ttccacaatc ggagattcgg caatccgcgc tgacaggaag gttccatccg
     2041 gcaacggaaa ttgaaggatt gcctggctct ggcgaatcgg gagctgacct tctttgggcg
     2101 cacggttgag tgtttgctca atcagactgg aattgagttg aaacacccgc tggttgttgg
     2161 cggttgcctg agacaggctt gagcccgtcg ctgaagcgag tgtcgtccac aatggattgg
     2221 ctgaaactgg cttgaggaag ccaacatttc cccagtgtga gatcaacaag ctgctgatca
     2281 acacaccaca acctacaatc gagctcaccc acaatcgttg catagatgcc tcacctgaaa
     2341 ggaattaaga gttccttcgt tataaccatt tgtggccgag aagcaagcca ttttgtttga
     2401 ttctcaggca tggagtctgg cacaagacgc tgtgctggtt cttcaaaaga aaaatctgag
     2461 tgatgcccac aatttgatat attgcccgca cagaaaatca agccaaagga aatgcaacca
     2521 aatggagttt caatctggtc aatcacttct tattcggccc gaacaacccg gtgatgctgc
     2581 cggtgtgcgg cgtgtcaatg aactggcgtt caatcaactg gatgaagcca atctcgtcga
     2641 agctttacac aaggtggccg tggggttgat ttcactggtg gcggtgcttg atgaccagat
     2701 tgtcggccat attttgttta gtccggcaac gattcaggga gcaggcggtg aatttcacgc
     2761 tattgggctg gcaccgatgg cagtcttgcc tgaatatcaa aatcgtggaa ttggctcgca
     2821 actggttcgg gctggtctgg ccgaatgccg ccgtcagggg caccgggtgg ccattgtttt
     2881 agggcatccg aagttctacc cacgatttgg ttttgttcct gcccggccca gagggatcac
     2941 ctgcgagtat gatgttgcag atgatacctt catggtcgct gaactcgaac ccggtgccct
     3001 ggctgggtgc accggattgg ccaaatatca cccggcattt gcaatggttg gataaataac
     3061 cgaatgaaga atgcagaatt gagagtgaaa aataaggaga aagaagttgt attcattttc
     3121 cttctgctcg attttagcca cgaatgtgtc aggaaagacc aacacgccgg atcaggtcgt
     3181 catatcgcgg atctgagcgc aatggaagta actctgggtc atactggagg tagctcaaat
     3241 aaaccgaatg cgtttgatat gcctgttcca gccacttgaa tgcctgatct ttgtcgttga
     3301 gagcaacaca cataagcgcc ataaaatggg ctgagtccgg tgtttccttt tggtgtggta
     3361 ctattctggc cagtaaattt tgtgcttcgg ctttgcggcc tgctcgaatc aacaaataga
     3421 cgcgataaaa atccatcaac acatcaggtg ggattttgtc caattcagcc agtgcctgag
     3481 gatatttttt ctggtaggaa taaaccagcg cccgcagggt gaagagttga gaatcggttg
     3541 ggtgcctggt cagggcttcc tgaatttcgc gatgggcgag atcaaactgg ccgtttagcc
     3601 cataagagaa ggacaagagt ccccggactg gactgagcac tggatcaagt tccctggctt
     3661 tgatcaagat aggaatggat tctggcggat tgaatgtttg gtaatactct ccgaaccaca
     3721 tcaacgtggt ggctagattg gggttgagtt caagcgcctt ttggagatcc tgtttggcgg
     3781 tacgatccca taaatgcacc ttggcgtgtc cgagcgctgc ataggcttca ggtaatgaag
     3841 tgtcgagcga caatgctttt tcggccatgg ctttggcttt tggaagcgct tcattggacg
     3901 gcaaaaacgt tccggccccg gtacagtaag cgtcggcgag tccggcgtag gccagggcat
     3961 atctgggatc aagttcaatg gcatggttga aatatcgaat ggctgtttcc aacccgtctt
     4021 tggtgaaaag cagggtgtag tagcgacctt tgacatattc ctgataggcc agggcattct
     4081 cagtatagtg tttggtgagt cggcgctgtt cctcatctga gagcttgtag gaaagggcct
     4141 gtgtcacctg ttctgaaata tgatcctgga gctgaaaata atctgagcag gtttcatcac
     4201 attgaaatgc ccagaccgtc tgactgttac tgacctgaat cagtcgcact gtgacccgca
     4261 cctgatcccc catctgttga atgctgccgt caaggactgt ttccacctgg agttctttgc
     4321 cgacctcggc tgcttctttc gatgaactgg caaacggtaa gacggatcgt gttgggcgaa
     4381 caaccagttg ctgcaggttg ctcaatcggg taatcagcac atcggtcagc ccaatactca
     4441 gatattcatc gtttccctga ggctgcagca ctttaaacgg caagaccgcg attgacctgg
     4501 ctgaagagtg gggttgaggt agcgatgggc gaggccacat cagaaatccc ccaagcattc
     4561 caataattac aaccataccg ataaggaaag ccgtccgagg ggaaagccac cccaggcggt
     4621 tggggagaat ggtttctacc tgagcgggcg ctgtggtcgt caccggatga agctgctctg
     4681 ataccaaatc agacgctg
//
LOCUS       DAOQDO010000148         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12113_length_4697_cov_3.268203, whole genome shotgun sequence.
ACCESSION   DAOQDO010000148 DAOQDO010000000
VERSION     DAOQDO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_4697_cov_3.268203"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..2353)
                     /locus_tag="PKZ53_03495"
     CDS             complement(227..2353)
                     /locus_tag="PKZ53_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HNJ39529.1"
                     /translation="MFSILQSHLAAFDDAAFEALANKGLVRRARKDLQSGVQPDVVAD
                     TEQELVLRIAETTVTLTEQGPQKARCTCPAVDICRHILMACFWVKDSPVPTPEVTPAQ
                     PEPVEPPATITNEATSDDFALATDQLLVVSLEALTRWAGKTVLKQARSALLSSPKLKV
                     SYLGMVVFQIPDFNVTCRYFSQTGLTGMLCSCKAREVCFHRVLAVLLFQQRHGKTLDE
                     VSLAPPTLKESNETPRSRADILSTARKFLEEIVAIGLCHLTETTQQRLATLAISATGV
                     HLPRLALGLRTLSSEVGLQLQRNARADLHRFFLTLARTHALCRALAHTDENHSRSDLI
                     GQHRTVYHEVGTLDLTGVGAYAWETPSGYAGLTVLFWDNASGQWHTWSEARPVFQGVE
                     FDPQARYLQEGPWQGMLNPAQASQHRFALFHSRRNPQFRLSGSSHSQAFLKKMTNPRE
                     LQFGSRVFSNWNQLRVYATTIWPMGLTEPNPNASLVVIQPASWGNRWFDETEQTLFWE
                     LLDDTGDEIPLVVPYRKLDQPRMKHLETLSPPTAAIWGVVVRLELHRSHLVLSPISLL
                     RLPDGDAPTVVNLGLTETTSHLPKKEPGSIIESELEAEIEPGIEIADDVQSPISGWLQ
                     SLIDTLQSTAEAGGTTLFSSHATTLTTLAKHFESIGITTVTQTITPLLDTPSVRPGAI
                     LQARYVGQLCLEAEQKLIFGQSSKKK"
     gene            complement(2458..3342)
                     /locus_tag="PKZ53_03500"
     CDS             complement(2458..3342)
                     /locus_tag="PKZ53_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39530.1"
                     /translation="MPQFPIFSKMKWFWSGTSGKTIAILMHEQDRNADRFQWAISYLA
                     EVWEEDGHRVRYVFGINKFVPADLAILHVDLSVVPDEYIKFGRQYPVTLNGEIKDIRK
                     SVISQNLVRPGDQYAGPVIIKSDLNYGGFPERRLAGKLAEASQTGIDSPMDYQILENL
                     QAVPEAAWNSPDLVVEKFLPEMDGAFYCIRNYIFLGDQANCTRRFSRRPIVNSQTQTR
                     SEDIEPHPAIVGLRRKLGFDYGKFDYVIHNGQPILLDANKTTGAATRSKKSPVQAHRR
                     MRARGLYEFFERRTNSAN"
     gene            complement(3602..>4697)
                     /locus_tag="PKZ53_03505"
     CDS             complement(3602..>4697)
                     /locus_tag="PKZ53_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ39531.1"
                     /translation="QLSCQMNQRQSRLERALDSLYSREYTGRGVRQTDESQSDPIRPK
                     PAGSLDPSQLNIPTWLNEVRELFPHDTCELIEKHALTRYGLTELVTDPKVLERLEPNL
                     DLLKMILTFRGQMKGQVVEVARKIVRQVIEEIKRKLEADIRLKLLGKLNRFQASPLKI
                     AQNLDWRGTIRRNLKHYDPEQKKLLIQQVRFFSRVERHLPWTVILCVDQSGSMVGSVI
                     HSAVMAGILAGLPAVKVHLVVFDTSVVDLTDRADDPVETLMSVQLGGGTDIGQALHYG
                     EQLVDIPHRTVLVLVSDFAEGASPTQLLAVSRRLREAGVLTLGLASLDEQANPFYDHE
                     MANQLAETGMHIAAMTPTHLAEWLAKRMKN"
BASE COUNT         1138 a         1117 c         1289 g         1153 t
ORIGIN      
        1 ttggtcgcag ttcaaaaaat tcatcttcct ggcgggataa agactcgaca tttttgtgtg
       61 atgttcagaa agaaccggcc tgtgaggacc acccgctcag gcagatggtc ctgacaggtc
      121 cttataccat tttggaatga attgctcgta ttagcgaata gttaagtaac tcaaggaaat
      181 agggttagtg aactactgac tacgggctac tgactacaaa ctgatattat tttttctttg
      241 acgattgtcc aaagatcagt ttttgttcgg cttcgagaca aagctggccg acataccgtg
      301 cctgcaaaat cgcacccgga cgaacggaag gggtgtccag caagggcgtg atcgtttggg
      361 ttaccgttgt tattccaatg ctttcaaagt gtttggcaag tgttgtcagg gttgtggcat
      421 gcgatgaaaa cagggttgta ccacctgctt cagccgttga ttggagcgtg tcaatcagag
      481 actggagcca gccagagatc ggagattgaa cgtcgtctgc aatctcaatt ccgggttcaa
      541 tctcagcttc cagttctgat tcaatgatgc ttccaggttc tttcttcgga aggtggctgg
      601 tggtttcggt gagcccaaga ttgaccactg ttggggcatc gccatctggc aacctgagca
      661 gggaaatcgg cgagagcacc aggtgtgagc ggtgcagttc cagccgaacc acaacgcccc
      721 aaatggctgc ggtgggtggt gacagtgttt cgaggtgttt cattcgcggc tgatccagtt
      781 ttcgataagg aaccacgagt ggaatttcat cccctgtatc atcaagcaat tcccagaaaa
      841 gggtctgttc ggtctcatca aaccagcggt tgccccagct tgccggttgg atcacaacca
      901 gacttgcgtt cggattcggt tcagtcagcc ccattggcca gatggtggtg gcgtagaccc
      961 tgagttgatt ccaatttgaa aacaccctgg acccaaactg taattctctt gggttggtca
     1021 tctttttgag gaacgcctga ctgtgagaac tgcccgagag tcgaaactgt gggttccggc
     1081 ggctgtggaa gagcgcgaac cgatgctgtg acgcctgggc tggattgagc atgccctgcc
     1141 acggaccttc ctgaagatac cgggcctgtg ggtcaaactc gacaccttga aagaccgggc
     1201 gagcttcgga ccaggtatgc cactggccgg aagcattgtc ccaaaagagc acggtcaacc
     1261 cggcatagcc tgacggagtt tcccaggcgt aggcgccaac accggtgaga tccagcgtgc
     1321 cgacttcgtg atagacggtc cgatgctggc caatcagatc acttcgggag tggttttcat
     1381 cggtatgggc cagtgcccgg cacaaggcat gggttcgggc cagggtcaaa aaaaagcgat
     1441 gcaggtcggc acgagcgttg cgctggagtt gcaacccaac ttcactcgaa agagttcgca
     1501 acccaagcgc cagtcgtggg aggtgaaccc cggtcgctga gatggccagc gtcgccagtc
     1561 gttgttgggt agtttcggtc aaatgacaca atccaatggc gacaatttct tcaaggaatt
     1621 tccgggcagt gctcagaata tcagcccggc tgcgtggtgt ttcattcgat tctttgagtg
     1681 ttggtggtgc cagactcact tcatcgagtg ttttcccatg acgttgttgg aagagcagca
     1741 cggccagaac ccggtgaaag cacacttcac gcgctttgca ggaacacagc atccccgtga
     1801 ggcccgtctg agaaaaatac cggcaggtca cattaaaatc aggaatctga aaaacaacca
     1861 ttcccagata tgaaaccttg agtttgggac ttgaaagcaa agcactccgc gcctgtttga
     1921 gaactgtttt gccagcccag cgggtgagcg cctccagaga caccaccagc aactgatcgg
     1981 ttgccagcgc aaaatcatca cttgtggctt cattggtgat tgtcgcaggt ggctctactg
     2041 gctcaggttg agccggagtc acttcaggcg ttggaactgg tgaatctttc acccaaaagc
     2101 aggccatcaa aatatggcgg cagatatcaa cagcggggca ggtacagcgg gctttctggg
     2161 gaccttgttc ggtcagggtc acggttgttt cggcaatccg gaggacaagt tcctgttcgg
     2221 tatcagccac aacgtcaggc tggaccccgc tttgtaaatc cttgcgcgcc cgccgcacca
     2281 gtcctttatt ggcaagagct tcgaacgcag cgtcatcaaa ggcagccagg tgggattgaa
     2341 ggatggaaaa catggaagag ggttcagggg tcagggttga gaaaaactgg attccgagtt
     2401 acacccattg tgccaggggc tggttggaaa ttcgatctca actccgcgac ttgcgattca
     2461 gtttgcacta ttggttcttc gctcaaagaa ctcgtacagc cctctggccc gcattcgccg
     2521 atgggcttga accggagact tttttgaccg ggtggcggca cccgtggttt tgttggcatc
     2581 aagcaaaatc ggctggccat tgtgaatgac atagtcaaat ttgccgtagt caaatccaag
     2641 tttgcgccgg agcccaacaa ttgcaggatg gggctcaatg tcctcactcc gggtttgggt
     2701 ctggctgttg acgattgggc gccgtgaaaa acgtcgggtg cagttggcct ggtcaccgag
     2761 aaaaatatag ttgcgaatac aataaaacgc tccatccatt tctggtagga atttttctac
     2821 caccaggtca ggggaattcc aggctgcttc aggaacggcc tgaaggtttt ccaatatctg
     2881 ataatccatt ggggaatcaa tcccggtttg ggatgcctcc gctaatttcc ccgccagccg
     2941 tcgttcggga aagccaccat agttcaaatc tgatttgatt atgaccggac ccgcatattg
     3001 gtcaccaggg cgaaccaggt tttggctgat aactgatttg cgaatgtctt tgatttcacc
     3061 attgagtgtg actgggtact ggcgaccaaa tttgatgtac tcatctggca ccaccgacag
     3121 gtctacgtga agaattgcca gatcagccgg aacaaatttg ttgattccga acacataccg
     3181 gactcgatgg ccgtcttctt cccacacttc tgccagataa ctgatggccc attgaaaccg
     3241 atctgcattc cggtcctgtt cgtgcatcag gatggcaatc gttttgcctg aagtcccaga
     3301 ccagaaccac ttcatttttg aaaaaattgg aaactgcggc ataaaatgaa tgaagaatga
     3361 agaatgaaga attgagaatg aagaattgag aatgaagaat tgagaatgaa gaattgagaa
     3421 tgaagaatga agaatgaaga atgaagaatg aagaattgag aatgaagaat gaagaaagaa
     3481 gaaagtgcct ggtgtttagt atctggtacc tggtgcttag tactttggaa ccaatgtttt
     3541 caaagaacca agaaccaaga accaagaacc aagaaccagg cactttcttc attctcaatt
     3601 ctcaattctt cattcgtttg gccagccatt cggccagatg cgttggtgtc atggcggcga
     3661 tgtgcattcc ggtttcagcc agttgattgg ccatttcgtg gtcatagaat gggttggcct
     3721 gttcatccag cgaagccagg ccaagggtca aaacaccagc ttcgcgaagt cggcggctga
     3781 cggccaggag ttgggtgggt gaagcgcctt cggcaaaatc actcaccagc accagcaccg
     3841 tgcgatgggg aatgtcaacc agttgttcac cgtagtgaag tgcctgacca atgtcagttc
     3901 cgccgccaag ctggacactc atcagcgttt caaccggatc atcagcacgg tcagttaaat
     3961 caaccactga ggtatcaaac acgaccagat ggacttttac ggctggaagc ccggccaaaa
     4021 tcccagccat gacggcgctg tgaatgactg atccgaccat gcttccgctt tgatcaacac
     4081 acaatatcac ggtccagggg agatgtcgtt caacccgtga gaagaagcga acttgttgaa
     4141 tcagaagctt tttctgctct ggatcatagt gtttgaggtt gcggcggatc gtgcctcgcc
     4201 agtccaggtt ttgggcaatt ttgagtgggg aagcttgaaa gcgattgagt ttcccaagca
     4261 gtttcaagcg gatgtcggct tcaagtttgc gtttgatttc ttcgattacc tgccggacga
     4321 ttttccgggc gacttctacc acctgacctt tcatttgacc acgaaaggtc agaatcattt
     4381 tgagtaaatc caggtttggt tccagtcttt ctaaaacttt ggggtcggtg accagttcag
     4441 tgaggccata gcgagtaagt gcgtgttttt caatgagttc acatgtatcg tgcgggaaaa
     4501 gttcgcggac ttcgttgagc cacgtgggga tattgagttg acttggatca agtgacccgg
     4561 ctggtttggg tctgattgga tcgctctggc tttcatcagt ctggcgaacg ccccgtccgg
     4621 tgtattcgcg tgaatagagg gaatcgaggg cccgttcgag ccggctctgg cgctgattca
     4681 tctggcatga aagctgg
//
LOCUS       DAOQDO010000149         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12125_length_4694_cov_3.074801, whole genome shotgun sequence.
ACCESSION   DAOQDO010000149 DAOQDO010000000
VERSION     DAOQDO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12125_length_4694_cov_3.074801"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKZ53_03510"
     CDS             complement(<1..497)
                     /locus_tag="PKZ53_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ39532.1"
                     /translation="MSSEPNNTPSNPSPAEPAKKQRVRLPRLKAADFQHPWDVAALET
                     VKRAKGLDLLVRKLNEYGFERWYYINNIADNVRVSQRQCKSIHDMLRESCDILGVTVE
                     PQLYLNQNPNVNAFTFGTQQPFIVINSALVDFLTEDQLLCVIAHEVGHIKCGHVLYKM
                     MANFLS"
     gene            881..2815
                     /locus_tag="PKZ53_03515"
     CDS             881..2815
                     /locus_tag="PKZ53_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39533.1"
                     /translation="MHQNHNPLHLAALLEAARLLHGSLDPDELLQRLFHLVLDHLSVA
                     RALVALQDPQGTMRLAQVQGFPYLKLGDEFDHQKLSHTTLAFYPIGNGSQPIGILGLA
                     ERTYSPILADDQAFLTHLIEIAGNALTNARAYQRLRSAQQTIQERKQNLQTILNLQRE
                     LQSLHDPDVIAQYLVTTLAERWAIAKFAFALWKTGHPEIHDERNLTFPPQHLFFQFPG
                     ELPPAALVSDLPESDLKATLGAQKVQLLFSIVLDDSLVGAIIFGPRLKHTPFQPNDLE
                     FGTELIAQAAIALANAWKTRDTIRHQQLAAFLSAQQGYQPAFEVLGNSLSGLVINGQY
                     ELEQPLAAGSSSIVYRATQVSLNRPVAVKLLRPPLPARAPHLPEQTPLEAVVARTLTH
                     PNAVQMIDSGISETGFAYLVMELLEGRTLAEELRHSGRLTIKRALTLALPLCHVLAEA
                     HRRGILHRDLKPDNIFLVSSGTEETIKVIDFGHAKLLDPQRVEPPPAALFSGLMIGNP
                     TYLAPERLQNQPYDTPSDMYSLGVILYEMLCGQPPFTASDGNPLTIALAHLTTAVPSP
                     LELNPALSPQIERLLQQALDKSPARRPTAENFAQALLHALADLEPKPKPNVVKLPPPP
                     PEEKKPFSFSLKPLLRRFKY"
     gene            3044..4006
                     /locus_tag="PKZ53_03520"
     CDS             3044..4006
                     /locus_tag="PKZ53_03520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ39534.1"
                     /translation="MTSGFQVPEPGTRTMHDLLIIGGGPGGISAALWAKRLGCNPLLL
                     EANAQPGGQLLRTYNRLIDCPGFWGLTGIEIAGRLEEHLRSLEVAWRVNTPVTQIDAS
                     RHTIWSGEEKFHARALILALGVVKRRLGIPGEREFAGRGVSTSATRDAVLYAGKPVAI
                     VGGGDGAFEEALMLADVGCQVTLIHRSDQFRARPMYLRPVLADPRISVKPFTRLTRIV
                     GDDTVTGVELEVVRPDEPVTVEQLAVSGVFLTLGTEPATRLVASQLDLDSEGFIVTDR
                     WGATSAPGVWAVGDACHPLLPSLSTAFGQGATAAKAAYNWLQER"
     gene            complement(4139..>4694)
                     /locus_tag="PKZ53_03525"
     CDS             complement(4139..>4694)
                     /locus_tag="PKZ53_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39535.1"
                     /translation="RDQEANDEIDQKIYRTALEAAKRKFSPEFMNRIDKVVVFRSLKE
                     HHLQMILDLELKAVQDRIMKSASEKFVFKCSDAAKRFLLDEGIDFKYGARHLKRAIER
                     FLVYPISNLVATEQVHTGDLVLIELAEDRTSLAFVKEEGGALILAPGTEYAKIFGLED
                     TDPGERFIPARVASASIHPRESNS"
BASE COUNT         1082 a         1261 c         1188 g         1163 t
ORIGIN      
        1 gacagaaaat tggccatcat tttatacagc acatggccac atttaatgtg cccgacttcg
       61 tgggcaatca cacatagaag ctgatcctct gtcagaaaat ccaccaacgc cgagttgatg
      121 acgataaatg gctgttgcgt gccaaaggta aaggcattca cgtttggatt ctggttgagg
      181 tacaactggg gctcgacggt tacgccaaga atatcgcacg attcgcgcaa catatcgtga
      241 attgatttac actggcgttg tgatacccgc acgttgtcgg caatgttgtt aatgtagtac
      301 caccgctcaa acccatattc attcaattta cgcaccagca aatcgagccc tttggcccgt
      361 ttcacggttt caagggcggc cacatcccac ggatgctgaa aatcagcagc tttgagcctg
      421 gggagccgga cacgttgttt tttggcaggt tctgcaggac tcggattcga tggagtgttg
      481 tttggttctg aactcatatt tttgggctta gggcttgggg cctggttctt cgtgattggg
      541 gctttgtttt tcgtgattgt ggctttgttc ttcgtaattg gtgcttggtg cctggttctt
      601 cgttttggga aggaactggt tcgcaggtac caagtaccag gtaccaagta ccaagcacca
      661 agcaccaagt accaagtacc aagtaccaag taccaaatac caagtaccaa gtaccaagca
      721 ccaggtacca ggcactaaat tcgtccgtga tgtccttttg gtctcttcaa ccttgggtta
      781 gaatcaggtt tcccaagatt ttttcgatca acagcaaaca catcttgcct gacactctag
      841 ctgatgggaa cggtcatttc cattaaaacc tgggcttgct atgcaccaaa atcacaatcc
      901 gctccatctg gctgccctgc ttgaagccgc gcggttgctg cacggatcac ttgatccgga
      961 tgaattgctg caacggctgt ttcatttggt gctggatcat ttgtccgtgg cgcgggccct
     1021 ggttgcattg caggatcccc agggcaccat gcgactggct caggttcaag ggtttccgta
     1081 cctcaagctc ggcgatgaat ttgaccacca gaagctcagt cacaccacgc ttgcatttta
     1141 cccaattggc aatgggtcgc agccgattgg gattttgggc ctggccgaac gaacctactc
     1201 gccgattttg gctgatgatc aggcatttct cacccatctg atcgaaattg ccggaaatgc
     1261 tctgaccaat gcccgtgcct atcagcggct gcgttctgcc cagcagacca tccaggaacg
     1321 aaagcaaaac ctgcaaacca tccttaacct gcaacgtgaa cttcaatcac tgcacgatcc
     1381 agatgttatt gctcagtatc tggtgacaac gctggctgag cgctgggcca tcgccaaatt
     1441 tgctttcgcg ttatggaaaa ccgggcatcc tgagatccac gacgagcgaa acctgacctt
     1501 cccaccccaa cacctcttct ttcaattccc aggggagctg ccaccagccg cactggtttc
     1561 ggatcttccg gagagtgact tgaaggccac tctgggggct caaaaagttc aacttttatt
     1621 ttcaattgtg cttgatgaca gcctggtggg ggcgattatt tttggtcccc gcctcaaaca
     1681 tacaccgttt caaccaaacg acctggaatt tggcaccgaa ctcattgccc aggcagccat
     1741 tgcgctcgcc aatgcctgga agacacgcga taccattcgc catcaacaac ttgccgcatt
     1801 cttgagtgcc cagcaaggct accagccagc ctttgaagta ctcgggaatt cactttctgg
     1861 gctggtcatc aatggtcagt atgaactgga gcagccactg gcagccggga gttcaagcat
     1921 tgtctatcgg gccacgcagg tcagcctcaa tcgcccggtt gcggtcaaac ttcttcggcc
     1981 accacttcca gccagagccc cccacctgcc cgaacaaaca cctctggaag ccgtggttgc
     2041 ccgaacactg acgcacccaa atgctgtcca gatgattgat tcaggaattt ccgagaccgg
     2101 gtttgcctat ctggtgatgg agctactgga aggccgaacc ctggctgaag aacttcgcca
     2161 ctcaggtcgt ctgacaatca aacgggcgct caccctggcg cttccgctct gtcacgtact
     2221 ggccgaggcc caccggcggg gaatcctcca tcgggatctc aaaccagaca atatttttct
     2281 ggtttcttca ggcacggagg aaacaatcaa agtcattgac tttggccacg ccaaactcct
     2341 tgatccacaa cgcgttgagc cgccaccagc cgccttgttt tcagggctga tgattggcaa
     2401 tccaacctat ttggccccag agcggcttca aaatcagcca tatgacacgc cctctgatat
     2461 gtacagtctg ggtgtgatcc tttatgaaat gctgtgcggg cagccgccct tcaccgccag
     2521 tgatggcaac ccattgacga ttgcactggc gcatctcacc acggcagttc cttcgccact
     2581 ggaactcaac ccggcgctat cgccgcaaat tgaacgactc ctgcagcagg ccctcgataa
     2641 atcccccgcc cggcgaccaa cggcggaaaa ttttgctcag gcattgctgc acgccctggc
     2701 tgacctggaa cccaaaccca aaccgaatgt ggtgaaattg cctccgcccc caccggaaga
     2761 gaaaaaacca ttttctttca gcctcaagcc actgttgcgg cgatttaagt actgaatcag
     2821 ttagggttca gggttcaggg ttcagggttc agggttcagg gttcagggtt ttcgagggaa
     2881 tggcaatttc aacctggact tgtttaatga tagtttcctt ctattcaaat tcaaaaatga
     2941 cgtaccagtc aaaaatcaat gacttgataa gttcttctga ttaaattact taactttcga
     3001 tttatctgac ttggaattcc caattgtttc accagcacag tgaatgacct ctggttttca
     3061 ggtcccggaa cccggaaccc gaactatgca cgatttgctc atcatcggtg gcggtccagg
     3121 tggtatcagc gcggcccttt gggccaaacg acttggatgc aatccacttt tactcgaagc
     3181 taatgcccaa cctggcggcc agcttttaag aacctataac cggctgattg attgtcctgg
     3241 gttttggggg ctgaccggca tcgaaattgc agggcgactg gaagaacacc tccggagtct
     3301 ggaggttgcc tggcgtgtca ataccccggt gacccaaatt gatgcatcac gccatacgat
     3361 ctggagtggc gaggaaaaat ttcatgccag agcattgatt ctggcccttg gtgtggtcaa
     3421 acgccggttg gggatccctg gcgaacgtga atttgctggc cgtggtgtca gtacttctgc
     3481 cacacgggat gcggtgctgt atgcgggcaa acctgtggcc atcgttggtg gaggggatgg
     3541 cgcttttgaa gaagctctga tgctggctga tgttgggtgc caggtgacat tgattcatcg
     3601 ctcagaccag tttcgggctc gaccaatgta tctgagaccc gttctggctg atccccgcat
     3661 cagcgtaaaa ccctttaccc gattgacccg catcgtggga gatgacaccg tcacaggtgt
     3721 cgaactggaa gtcgtccgac ctgatgagcc agtgacggtc gagcaactcg ccgtgagtgg
     3781 tgtctttctc acacttggta ctgaaccggc cactcgtctg gtggccagcc agcttgatct
     3841 tgattcagaa gggtttatcg tcactgaccg ctggggtgcc acttcggcgc ctggggtatg
     3901 ggcagtcggt gatgcctgcc atccattgct cccaagtctg tcaacggctt ttggtcaggg
     3961 ggcaaccgct gccaaggcgg cgtataactg gctgcaggaa agatgacagg atgacaaatg
     4021 acaggggaca aaatgacaaa tgacagagtg acgaaagaat acaaagcgtt ggaacgtttc
     4081 attccgtcag cttttcactc agtcacttct gtcatttatc acccggtcag cttgtcaatt
     4141 acgagtttga ttcgcgtggg tgaattgacg ccgatgcaac tcgggcaggg ataaagcgtt
     4201 cgccagggtc ggtgtcttcc aggccgaaga tcttggcgta ttcggtgccg ggagccagaa
     4261 tcagtgcccc gccttcttct ttgacaaagg ccaggctggt gcgatcttca gccagttcga
     4321 tcaagaccag gtcgccagtg tgaacttgct cggtggccac caggttggaa atcggataca
     4381 cgagaaaccg ttcgatggcc cgtttgagat gccgcgcccc atatttgaaa tcaatccctt
     4441 catcgagcag aaagcgcttg gcggcatctg aacacttaaa gacaaatttc tctgacgcgc
     4501 tcttcataat gcgatcctga accgctttca attcaaggtc caaaatcatc tgcaaatgat
     4561 gttctttgag tgaacggaag accacaactt tatcaatccg gttcataaat tccggagaga
     4621 actttcgttt ggcggcttcg agggcagtgc gataaatctt ttgatcaatc tcgtcattgg
     4681 cttcctggtc acgt
//
LOCUS       DAOQDO010000150         4693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12129_length_4693_cov_3.372790, whole genome shotgun sequence.
ACCESSION   DAOQDO010000150 DAOQDO010000000
VERSION     DAOQDO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12129_length_4693_cov_3.372790"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..4040
                     /locus_tag="PKZ53_03530"
     CDS             414..4040
                     /locus_tag="PKZ53_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39536.1"
                     /translation="MPCSDSFQVVIARVGLCLCFLFGLSQSAAGQSPTLTSIKPDRTP
                     VGQIPAKVVLKGTGFETSSVVVINTTPVAASIKPSGKKIVIKDLPTEFFQTSGVLEIK
                     VIPATGLESNSIRLTVGSLNTIQLDASTELVVNVGTTVTLRAQVLDAQGVPLPDAVIT
                     YSSLTPDQATVDQAGVVTGIQTGAATIRLTSGEAIRDLVVTVTDVASIPSGIVGDGDI
                     KVDSQNRVYATDLRRHIIRSAPVGQPLAEFAGAVDLPGNIDGALTSSRFNGPLGLGLG
                     SNSPFVYLADTANQSIRRFNRETGTVETVVGLADVAAVASVDSWGPRGVVEAQPGELY
                     VTDADNHVVWKVRLGTTPDVSVFAGTVGQPGLLDGTGNNALFNAPQDLVSGTQQSILV
                     VTDRGNRLVRLIALPDGQVTTVGNIASARVLTRTNTYVPKQSVTFGDPQGVEIDSIGN
                     LYVTDGNTVRVVELQDTTFVVSDLAQSGTFQNATGISLASGSAYVLDSGKQQVIRVAL
                     GAPPSISELNINEIKAGQTTELTINGSNFIPNTQVKIGSQFVYAVRVENASRIRCLLP
                     PQPESGPLQITVQHRGGSATTTVNVVSAPFFQLALEPSERSVTAGQMVEYKVRILRTN
                     FNGNVALSLSGLPAGTQARFLPNVTSAGESTLTIATAATVEPGKYPIRVSGLTTGVTA
                     TTDGLLVVTRPEPGSVSIAVNPSTKTIAPGQNATFQIALNRSNFVGPVKLLAEGVPAG
                     ARADFAPSELTGNSSNLTLTTLTSVAEGTYRITIKGLADNTPVQASALTLTIQKAATG
                     SGVRIVATPASLTINAGQSATYNLELQRTSFTGDVRLSVSGLPSGAVASFRPESTTGT
                     TSQLIVTTAASTPTGTVRLTISGSASGTSVTPTSVTLAINSSSGGGASVRFSVSPETR
                     TIQAGQTASFAVNLLRTSFTGEVRFALLGLPAGAQASFNPDRTTGNSTTLTVVTAASL
                     TPGSYVLTVSGTAFGATISPAAATLVVSGNGGGNGSVSLSANPVSQTVQAGNTAQYSI
                     RLTRTSFDGEVKFGIGTLPAGVTARFDPDRTTGNSSQLTLQVGGTVAGGTYQIPVSGL
                     ASGATVTGTMLTLIVNAAPQASVTISVSPSSSTIRLGGSASFSINLARMNYTGAVSLS
                     VAGLPAGITAVFNPESTLGNSAGLTLSARTDAPIGTYTIRINGATTGGVTINQATITV
                     TTQR"
     gene            4037..>4693
                     /locus_tag="PKZ53_03535"
     CDS             4037..>4693
                     /locus_tag="PKZ53_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNJ39537.1"
                     /translation="MSPKAKPVQTETESHQSLASRSWTLDQAFEHCRRVALGHYENFP
                     VGSLLIPKAHRPHVYSVYAFARAADDFADEGTLNQAERLSLMENWREQLVACINGKAT
                     HPVFLALAESIKVNRLPLQLFHDLLDAFTIDIVRSRHQTFDALLDYSRGSANPVGRLI
                     LLLFGHRDEQLARWSDDICTALQLTNFWQDVLVDLAKDRIYIPLAEMERFGYAEADLK
                     A"
BASE COUNT         1063 a         1293 c         1248 g         1089 t
ORIGIN      
        1 ggggttaatg ttcaggtacc gggatggcgt taccgacgat caaaacctca gaactgatgg
       61 ggtgctgaga agtcggatct tctggcatct tttcccaaat atcccagttc caatatgaaa
      121 agagatgcta aaactagcta aaggttggac ttatgtcaac gaggaagtca gggatgtgtc
      181 caaatctctg caattttacg gcctgatgcg aggctgatac cccaaaactt ccaagtgctg
      241 gaaacgtcat acgttaccgg gcacctttca gaatcaaacc cggcgccaac aatacaagaa
      301 gtgccttgtc ggctggaagg gtttggacca taatcacaga cccgtgtaac gcagtcatta
      361 actcatctca ccatgcagcc ctgaagccat ccccgaaaaa tccaggtttt tctatgccgt
      421 gctctgactc tttccaggtt gtaatcgccc gtgtcggttt gtgcttatgt ttcctgtttg
      481 gtttgagcca atcggcagcc ggccagtcgc ccacccttac ctcgataaaa ccagaccgga
      541 ccccagttgg ccagattccc gccaaagtgg tgttaaaagg aaccgggttt gagaccagtt
      601 cggtggtggt catcaatacc acgccagtgg ctgccagcat aaaaccttcg gggaaaaaaa
      661 ttgtgattaa agacctcccg actgaattct ttcagaccag cggagtgctt gaaatcaaag
      721 tgattccggc gaccggcctg gaatcaaata gcatccggct gacggttgga agtctcaaca
      781 cgatccagct cgatgcctca accgaactgg ttgtaaatgt tggcaccact gttacgcttc
      841 gggctcaagt gcttgatgcc cagggggttc cgctgccaga tgccgtgatt acatattcaa
      901 gcctcacacc ggatcaggcc accgttgatc aggccggagt cgtgactgga attcaaaccg
      961 gggccgcgac gattcgactg acttcgggcg aagccatccg agatctggtc gtgacggtca
     1021 ccgatgtggc cagtattcct tcgggaattg ttggagatgg tgatatcaaa gttgattccc
     1081 aaaaccgtgt ctatgccacc gacctgagac ggcatatcat tcggagcgct cctgttggcc
     1141 agccactggc tgaatttgcc ggtgcggttg acctgccggg aaatattgat ggtgcgttga
     1201 cgtcctcgcg gtttaacggc ccgctcgggt taggactagg aagcaattcc ccatttgtct
     1261 atctggctga cactgccaat caatcaatcc gacgctttaa tcgtgaaact ggaactgtcg
     1321 aaacggttgt tggtttggcg gacgtggcag cagtggccag cgttgattcc tggggaccac
     1381 gcggagttgt cgaagcccaa cctggagaac tctatgtcac agatgccgat aaccatgttg
     1441 tctggaaagt ccgactgggc accacacctg acgtttcagt ttttgctgga acggttggcc
     1501 agccaggact tctggatggg actggaaaca acgcgctgtt taatgcgccg caagatctgg
     1561 tcagcgggac gcagcaaagc attctggtcg tgacagaccg tggaaatcgt ctcgtgcgat
     1621 tgattgcctt acctgacggc caggttacca ccgttggaaa tattgccagt gctcgtgttt
     1681 tgacccggac caacacctat gtgccgaaac aaagcgtgac gtttggcgat ccacaagggg
     1741 tggaaattga ttcgattgga aatttgtatg tcaccgatgg aaatacggtc cgggtggttg
     1801 aactccagga tacaactttt gttgtcagtg atttagccca aagcggcact tttcaaaatg
     1861 ccacggggat ttctctggcg agtggttcgg cctatgtgct ggattcaggg aaacagcagg
     1921 tcatccgggt ggcgctcggt gccccacctt caatttccga actcaacatc aacgaaatta
     1981 aagccggtca aacgactgag ttgacgatta acgggtcaaa cttcattcca aacacccagg
     2041 tcaaaattgg atctcagttt gtgtatgccg tgcgggttga aaacgcttcc cggattcgat
     2101 gcctgcttcc accacaaccg gaaagcggcc cgctccaaat cacggttcaa catcgtggag
     2161 gttcagccac caccacagtg aatgtggtga gcgcaccatt tttccaactg gcactcgaac
     2221 cctctgagcg atcagtgacg gcaggtcaaa tggttgagta caaagtgcgg atcctgcgaa
     2281 ccaattttaa tgggaatgtt gccctgtctc tcagtggact cccggctggc acacaggctc
     2341 gattcctgcc aaacgtgacg agtgctggtg aatcaacctt gaccatcgcc actgccgcca
     2401 ctgttgagcc cggcaaatat ccaattcgtg tcagtgggtt gaccacaggc gttaccgcca
     2461 cgacagatgg attactggtg gtgactcgac ccgagccggg tagcgtttca attgcggtca
     2521 acccatcaac caaaaccatt gctccggggc aaaatgcaac atttcagatt gccctgaacc
     2581 gctcgaattt cgtgggcccc gtcaaattgc tggctgaagg tgtaccagcc ggtgcccgag
     2641 ctgattttgc tccgtctgag ttgaccggca acagttcaaa cctgaccctc accacactca
     2701 ccagtgtggc tgaaggtaca taccggataa caatcaaagg gctcgccgat aacacacccg
     2761 ttcaggcgag cgcgttgacg ttgaccatcc agaaagcggc aaccgggtcc ggcgtgcgga
     2821 ttgtggccac tccggcttcc ctcaccatca atgcaggcca atcggccacc tataatttgg
     2881 agttgcagcg cacaagcttc actggcgacg tccggttatc ggtatctggt ctcccctctg
     2941 gggccgtggc aagtttccgg cctgaaagca ccaccggaac aacctcacaa ttaatcgtca
     3001 caacagcggc cagtaccccg actgggacag tcaggctcac gatttccggc tctgcctcag
     3061 gtacttcggt gacaccgacc tcggtgacct tggccatcaa ctcaagtagc ggtggcggtg
     3121 ccagtgtccg attttcggtt tctcctgaga cccgaacgat tcaggctggc cagaccgcca
     3181 gttttgccgt gaatttgctc cgtaccagct tcaccggtga agtccggttt gccttacttg
     3241 ggttaccagc gggagcacag gccagtttca acccggatag gaccactggc aattcaacga
     3301 ccttgacggt ggtgactgcc gccagcctca cgcctggcag ttatgttttg acagtttccg
     3361 ggactgcctt tggggccacg atcagtcccg ccgctgccac tctggttgtt tctggaaatg
     3421 gaggtggcaa cgggagcgtt tccctttccg ccaatccagt cagccagacc gttcaagccg
     3481 ggaacaccgc tcaatattca attcgattaa ctcgaaccag ttttgatggt gaggtcaaat
     3541 ttgggattgg taccttgccg gctggggtta cggcccgctt tgatccagat cgcaccacgg
     3601 gaaatagttc acaactcacg ctgcaggttg gcggtaccgt tgctggcggt acgtaccaga
     3661 ttccagtgag cggtctggcc tctggggcta cggtgactgg cacaatgttg acgttgattg
     3721 tcaatgcggc ccctcaagca tcggtgacaa tctcggtgtc accgtcttcg agcacaatca
     3781 ggctgggtgg gagcgcatcc ttctcgatca atctggcccg gatgaactac actggcgcgg
     3841 tctcactgag tgtcgctggt ctcccggctg gcataaccgc tgtttttaac cccgaaagca
     3901 cccttggcaa cagtgcggga ttgacgcttt cggccagaac tgatgctccg attggcacct
     3961 acacgattcg tatcaatggt gccaccacgg gcggcgtcac catcaatcag gcaaccataa
     4021 ccgtaaccac ccaacgatga gccccaaagc caaaccggtt cagactgaaa ccgaatcaca
     4081 tcagtcactt gcctcacgtt cctggacgct tgaccaggcg tttgaacatt gccgccgggt
     4141 tgcactgggg cactatgaaa acttcccggt ggggtcgctg ctgatcccaa aggcacaccg
     4201 tccgcacgtc tatagcgtct atgcctttgc ccgggcagca gatgattttg ccgacgaagg
     4261 gaccttgaat caggccgaac gcctcagcct gatggaaaac tggcgcgagc aactcgtcgc
     4321 ctgtatcaac ggcaaagcaa cccacccggt gtttctggca ctggcagagt caatcaaagt
     4381 caaccggctg ccgctccagc tttttcatga tttgctcgac gcctttacga ttgatattgt
     4441 gcggtcgcgc caccagacgt ttgacgcctt gcttgactac agccggggtt cagccaatcc
     4501 ggttggacga ttgattttgc tgctgtttgg gcatcgtgat gagcaactgg cccgctggtc
     4561 agatgatatt tgcaccgcat tgcaactcac caatttttgg caggatgtgc tggttgacct
     4621 ggccaaagat cggatttata tcccgttggc cgaaatggaa cgttttggct acgctgaggc
     4681 ggatctcaag gca
//
LOCUS       DAOQDO010000151         4689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12150_length_4689_cov_2.522443, whole genome shotgun sequence.
ACCESSION   DAOQDO010000151 DAOQDO010000000
VERSION     DAOQDO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12150_length_4689_cov_2.522443"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKZ53_03540"
     CDS             complement(<1..200)
                     /locus_tag="PKZ53_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39538.1"
                     /translation="MLVSAKPSAATPQTASHADPQDPSAPANTAASRSAFTKLREKKK
                     ATKSSCFPPSWKASGLCRIILKR"
     gene            225..2879
                     /gene="bamD"
                     /locus_tag="PKZ53_03545"
     CDS             225..2879
                     /gene="bamD"
                     /locus_tag="PKZ53_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNJ39539.1"
                     /translation="MREFGLLPRIQAQKPGALSETKKAQTGPSWPIGDNADDLSQLIR
                     EADTTWAALDEASATRNETTYQRILERYLELTEVDFFSDYQEPTDVQARRNSAIDQLD
                     ALTARKQGSSSEAVWSYIEARQAFDNQPEDQKEEPNLTKLDHNLRDNVAFLSAAVLYA
                     QQDYVAAATAFRKLARRYPTSEKREAALYLAALSEAKCTVAFWISDPETDSPTPDEDW
                     AGLRRAFQRVLNEYPNGRYKGDVLGWLGFMNLQFGNRPQALVYYYKQLSIDDPIIQRQ
                     AVKSLRLVRHDATEVEMHRLQQLLSHDPETALTYTCHELYNYTQMIDKFGADCSCGDG
                     EDDDSWVQGQYQRIAGYSAKMMDNFPQSNLSGSFALRAAMANLESGDHATAIKLARQA
                     LRLGLNGIERARGLWVLGVAQYRHRQLDAAAKSFTQLIQEFPTGDLVEGTRRHLAMLA
                     EELGRRDVALEQYLALNYQLDIAYLIDVMMTPEELRTFITTHPQSDELNELWYALGIR
                     YLRNHQWTDAREAFANVRTISDDIDEQYHWNIMTTYDYQNPETQLKPSPKSTETDIDW
                     KVNGIRSRWLHRDLQTIDDLEFLQSRIDSAQDDTQRAEAMYQFASYVYQGGSFLLYNP
                     AAWKGMRYSSIAVKNDFSLPNEEQQLWDYHLNHEAVAQAKTMFLAIVDAYPKSPAAPD
                     ALYTAAVCDERLLNFSPYWRAMSQTGRHAGNRWVTYDDVRRQFPGYQLPRGTFGWEPS
                     TRTVNGGPGFEPKPKPLPRKSRLERTLEKLAVWSEPVISFGSSLWQWYKGYLLNLPEF
                     SRKWSQWFWSVTSCLSLIPFTVLLWMLVNQRKQRFLDQLNIQEEPTNPKLQPRLYQAI
                     TISAHTLVHSAFVLLLWGLLSFTISLFG"
     gene            complement(3346..3627)
                     /locus_tag="PKZ53_03550"
     CDS             complement(3346..3627)
                     /locus_tag="PKZ53_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HNJ39540.1"
                     /translation="MPEVQLNQVLYQAQQLSPQDQLELIKCVVASLSKAMGAKENVEN
                     LKGNSGMCDSTTGNEILSSPRIPGLHAGEVWMSEDFNEPLPDEFWMGNA"
     gene            3854..>4689
                     /locus_tag="PKZ53_03555"
     CDS             3854..>4689
                     /locus_tag="PKZ53_03555"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39541.1"
                     /translation="MPLYRLLCSLLLLIVFHPFPVSAQSESTEKKESKVLQFRSIGPA
                     AGGRVARVAGIPGNPSIYYAAGAASGVWKTIDGGLTWKPIFDDQPISSIGSIAVAPSD
                     PNVIYVGSGEANIRGNVAAGNGIYKSTDAGKSWTHVWKQEGQIGTMIVHPKNPDVAFA
                     AVLGHAFGPNDERGVFRTTDGGKNWKQVLKKNADTGASDVCFDPSNPNILFAGLWQAR
                     RQPWELTTAGPGSGLYVSRNGGETWKQLTGNGLPEGIWGKVCVGVAPSDSRRVYAIIE
                     AE"
BASE COUNT         1150 a         1255 c         1149 g         1135 t
ORIGIN      
        1 cgcttcagga tgattcgaca caatccagac gctttccagc tgggcggaaa gcaactgctt
       61 ttcgtcgcct tcttcttttc ccgcaacttt gtaaacgcgg atcgggacgc cgccgtattc
      121 gctggcgccg acggatcctg cggatccgcg tgtgacgcgg tttggggcgt ggctgcggat
      181 ggtttggcgg agacgagcat tcggtcaggt ttaattattc aagcgtgcgt gaattcggct
      241 tactccccag aattcaggct caaaaaccgg gcgcactctc agaaaccaaa aaagcacaaa
      301 ccggtccaag ttggcccatt ggagacaacg ccgatgatct cagccagctt atacgcgaag
      361 ccgataccac gtgggcagcg cttgatgaag cctctgcaac ccgcaacgaa acaacctatc
      421 agcgaatttt agagcgctat cttgaactga ccgaggttga ttttttcagc gattaccagg
      481 aaccaaccga tgtccaggcc cgacgtaatt cggcgattga ccaactggac gcgttgaccg
      541 cccgcaaaca gggatcatcc agtgaggctg tttggagcta cattgaagct cgccaggcgt
      601 ttgataacca gccggaggat caaaaagaag aaccaaactt aacaaaactg gatcacaatt
      661 tgcgcgataa tgtcgcgttc ctgtcggcgg cggtcctcta tgcccagcaa gattacgtcg
      721 cagcggccac tgcttttcgc aagctggcca ggcggtatcc aaccagtgaa aaacgcgaag
      781 cggccctcta cctcgcggct ctttccgaag ccaaatgtac ggttgccttt tggatttctg
      841 atcctgaaac cgattcacca acacctgatg aagactgggc cggccttcgc agagcgtttc
      901 aacgggttct gaacgaatac ccaaacggtc gttacaaagg cgatgtgctc ggctggctgg
      961 gctttatgaa tttgcaattt gggaatcgtc ctcaggcgct ggtctattac tacaaacaac
     1021 tttcgattga tgacccaatc atccagcggc aggcggtgaa gtcgctcaga cttgttcgtc
     1081 atgatgccac cgaagtcgaa atgcaccggt tgcagcaatt actttcgcat gatcctgaaa
     1141 ctgctctgac ctacacctgt cacgaattgt acaactacac gcaaatgatt gataagtttg
     1201 gagctgactg ctcctgtggt gacggcgagg acgatgattc ctgggttcaa ggccaatacc
     1261 agcgcattgc tggctattca gccaaaatga tggacaactt cccacaatcc aacctgtctg
     1321 ggagctttgc cctgcgagca gccatggcaa atctggaaag tggtgatcat gcaactgcta
     1381 tcaaactggc acgtcaggcg ttgaggctgg gactcaatgg aatagagcgg gcacgtgggc
     1441 tgtgggtgct tggcgtcgct cagtatcgcc atcgccagtt ggatgccgcc gccaaatcat
     1501 tcacccaact gatccaggaa tttccgaccg gcgatctggt cgaaggcact cgccgccatc
     1561 tggcgatgct ggctgaagaa cttggccgcc gggatgtcgc acttgagcaa tatctggccc
     1621 tcaattatca actcgatatc gcctacctga ttgacgtcat gatgacccca gaggaactgc
     1681 gcactttcat taccacgcat ccgcagagcg atgaactgaa cgaactctgg tacgccctgg
     1741 gcatccggta tcttcgcaac caccagtgga cagatgctcg cgaagcattt gccaatgtca
     1801 gaaccatcag tgacgacatt gatgaacaat atcactggaa catcatgacc acgtacgatt
     1861 accagaatcc tgaaacccag ttgaagccga gtccaaaaag caccgaaacc gatattgact
     1921 ggaaggtaaa cggaatccgt agccgctggc ttcatcgtga tttgcaaacc attgatgatc
     1981 ttgagttttt gcaatcccga attgattctg cccaggatga cacccagcgc gctgaggcga
     2041 tgtaccagtt tgcgagttac gtgtatcaag gcggttcgtt tctgttgtac aacccggcag
     2101 cctggaaagg aatgcgctat tcgagcattg cggtcaagaa cgacttttca ctcccgaacg
     2161 aagaacaaca actttgggac tatcacctca accacgaagc cgttgcgcag gccaaaacca
     2221 tgtttctggc catcgttgat gcctacccca agagcccggc agccccggac gcactctata
     2281 ccgcagcggt gtgtgacgaa cgactactca acttcagccc gtactggcgg gcaatgtccc
     2341 agacagggcg tcacgctggg aaccgatggg ttacttatga tgatgttcgg cggcaatttc
     2401 caggctacca attgccacgc ggaacgtttg gctgggagcc gtcaacccga acagtcaacg
     2461 gcggtcctgg atttgaaccc aaaccgaaac cactaccgcg caaatcacgg cttgaacgaa
     2521 cgttggaaaa actggccgtg tggagcgaac ccgtcatcag ttttggttcc agcctctggc
     2581 aatggtacaa aggctacctg ttgaacctgc cggaattcag ccgaaagtgg tcgcaatggt
     2641 tttggagtgt aacatcgtgc ctctctttga tccccttcac cgttttactc tggatgctgg
     2701 tcaatcagag aaaacagcgt ttcctggacc aactcaatat ccaggaagaa ccaaccaatc
     2761 caaaacttca accacgtctc tatcaagcaa ttaccatttc agcacatacg ctggtccaca
     2821 gcgcgttcgt gttgctgctg tggggattgc tgagctttac gatcagtctt tttggataag
     2881 aggtcagagt gagtttttag tcagtggtcc gtagtcagta gttcactaag tttatttgat
     2941 tgaattactt gactgcgtac cttctttaat caagtgggaa aaagtcaaga gttccgcctt
     3001 caggcgggtt tagtattcca actttggtct aaatggacgc tttaaccgcc taaaggcaga
     3061 actcttagct ctaaatctca gttttcaaac aaaaaattgc cgctgattag gctcagaacc
     3121 aatgtatttg ctccgtcatt taaggcatct ataaatttgc aggaaaaggt tcagagtatc
     3181 gtctttagac gagaggtttt catcgttcgt cccactcggc aaacctcacg cctgaaggcg
     3241 ttactctgaa caggaatttt gttctgaaag gctatataac ttctggcgaa agcttccccg
     3301 gctcattgtc ccaccaaaga aatgcctgcg tatcaaaaag tggtttcatg cgttgcccat
     3361 ccagaactca tcaggaagtg gttcattaaa atcttcgctc atccagactt ctcccgcgtg
     3421 taagccagga atgcgaggtg atgaaagaat ctcattgcca gtggttgaat cacacattcc
     3481 actgttccct ttcaggtttt ccacattctc ttttgccccc atcgccttac ttaaactcgc
     3541 gactacgcac ttgatcagtt caagctggtc ctggggagaa agttgttgag cttgatataa
     3601 aacttgattc aattggactt cgggcatctt catctccttt cattgagacc tattttaatc
     3661 aaaccacatc cttgccttca tcctcaagtc ctggctgaac atcagctaaa ccattgctga
     3721 atttgtcttt ccccaagccc caagccccaa gccctaagcc ctaaattgct tgcactctcg
     3781 cctcgttccc tttaccttac cccattcctt cccgtcgcat ttcatatccc tcaatcagca
     3841 ggagaattca tcaatgcccc tgtaccgcct tttgtgtagt ttattgcttc tcatcgtttt
     3901 ccatcccttc cctgtcagtg cccaatcgga atcaaccgaa aaaaaagaat ccaaagtcct
     3961 ccagtttcgc tctatcggtc cggctgccgg aggccgtgtc gcccgtgtgg cggggattcc
     4021 tggcaatcct tccatttatt atgcggctgg ggcggcaagc ggcgtctgga aaaccattga
     4081 cggtggcctc acctggaaac ccatttttga tgaccagccg atttcttcaa ttggttcaat
     4141 tgcggtggcg ccgtctgatc cgaacgtcat ctatgtcggt tctggcgaag ccaacatccg
     4201 gggcaatgtg gcggctggca atggaatcta caaatctacc gatgctggaa agagctggac
     4261 acacgtctgg aaacaggaag gccagattgg aacgatgatc gtccatccga aaaacccgga
     4321 tgtggcgttc gcagcagtgc ttgggcatgc gtttggccca aatgatgaac gcggtgtatt
     4381 tcgcaccact gacggcggaa aaaactggaa gcaggtgctc aagaaaaatg ccgacacggg
     4441 cgcttcggac gtgtgttttg atccgtcaaa tccaaacatt ctttttgctg gactctggca
     4501 ggctcgtcgc cagccctggg agttaaccac ggcaggtcca ggaagcgggc tctatgtctc
     4561 acgcaatggt ggcgaaacct ggaaacaatt gactggaaat ggcttgccgg aaggcatctg
     4621 gggcaaggtc tgtgtcgggg tggctccctc tgacagtcgg cgggtgtatg ccatcattga
     4681 agccgaaaa
//
LOCUS       DAOQDO010000152         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12218_length_4674_cov_2.501624, whole genome shotgun sequence.
ACCESSION   DAOQDO010000152 DAOQDO010000000
VERSION     DAOQDO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12218_length_4674_cov_2.501624"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..>4674
                     /locus_tag="PKZ53_03560"
     CDS             183..>4674
                     /locus_tag="PKZ53_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39542.1"
                     /translation="MVLIVLSFSFWKSRADVIPTKTEDLQAQIEAALYTRAEFFGSQA
                     LVPIPTAEARTRMEALHAQYPQASLIARHLSQLDEKLGNPDLAEKEIEHYAELEHQSV
                     ESLELVAAFQNRQARFDHEAKTLERLLNLVPKTQKVSMLQRLMAHARKHNLTAYLGPQ
                     YYQNVLEQDPSVVSVIIELIDWLAGEKNYPEALKLVRQYKPHFPQNTSYFVVTEANLL
                     ANLGREKEAESVFSTALDAQWSEALAQSFDTFLDEHDRYRAFYFEVKEQFLQHPESFS
                     LLVKRLHYLERHYTGGSVKNDILRHEKARAERNQPLTPDELLIISHYLIEENQPDEAA
                     RYLYTVCLQTQVKPGDPIRAKVLYQLFEILMDAQNEQLSLTNNDLQFYQDIATADSHP
                     GLFGAVLSLIFSDSDVGDKFEEKQTNATRFFNKAAAFRIFSAYKQENPTSPELAQMYL
                     DIVRLYAATQELQIADATLVEFEQRHQNASQFPEVALKLADAYLARDNRREEQAVLQR
                     VLDYFGKNRKPGDQLLPVSPQIGVSTELTGPPPFQTQTQPQSPSGINIPNTKDSSTTE
                     NWSYSYSSFNNHLGLSAAVQPQTITYAIVLDRLVSSLFVQERRQDILSLYASELKKYP
                     DEQGLYEQWLQWLDQTNLAEEQLRVYREAITHFPTTLWHHRLARWLIRTKRDRELETF
                     SRQLISTVNDAETEAYLSNFIASRLNSSTEFDRNLCLGLYQMAHKRFPHNLMFVNGLL
                     RYYEQRKEWTEWRKLMAEYVFVSPGIRQEFLSHLASRKELRSSVEQARKKCATTTKNP
                     LELLPYKLFQADAAIRLSDFEGAVETYRELIRLYPNNAEFTLRLVALNRSFGQKDRTA
                     LTEAATIQHAYTEANPAISGLRTTSGELYAELGDYGQAKAEWEKLLDLGRGEPEIYLD
                     TATLYWDYFQYDDALHTIWRLREQLHNDSLYAYQAGAIHEAKHQRPEALVEYVKALNQ
                     ETTDYADRRKTRARLKVLYDRQDVPQALEQALAESYRTQPQPEWLVLGYAEFLKEVGQ
                     EAQAVNHLKTEVTQSRSQEFLTRARSFFRNSSHLDGEATTLHQLIASAKSHRFAISFH
                     LQLAELYKNHRDLPAADRVLRQLRQRYPLNYGVLTETADIYWRMGQSNQALEILKQGR
                     QRSIGRFAYRFARKLAARYLDLNQFPQAEQILRQLHQENPLDSGVFQELARMYVKTSQ
                     PAELGEVFQQSLSAIQKQDIEIQEIRYQVADVREKMITAFTQLKDYDAAIDQHIEIIN
                     REPDDEDRLNVALSYAKRYGGSERLLAYYQQTAKQAYKNYRWYLVLARIYEAKNDWPR
                     AIENYRTAIGNQPEKVDLYAAIAEAHLKLNQLNEAIKALRKAAELTNDDPVYLKKLIN
                     VLEQAGYHKEAAVVRQKLPEPPPPKQQTLSQQLAEAEQLRTTDRAKAIELYRKVFETY
                     ATNPYKNEINIGDLTSYVQTLHSEEPLDKIAGRLWDLRQKLITERESPTSLQAGTAQE
                     RIK"
BASE COUNT         1289 a         1318 c         1089 g          978 t
ORIGIN      
        1 tcgtcacggt aacggctgaa gatttcgccc acaatcaggc aagtactgag attgaacttc
       61 aggtctacta aagcggtgtc aagccaccgc actccacagg cgtcaagccg ccgaaacgaa
      121 aagcggtgtc aagccaccgc actccaaaga ttatgaaacc gcaattcctc gctgcattcg
      181 ctgtggttct gattgtcctg agtttttctt tttggaaaag ccgcgccgat gtcattccaa
      241 caaaaactga ggacctgcaa gctcaaattg aggctgcact ctatacccga gctgaatttt
      301 ttgggtctca ggcactggtg ccaattccaa ccgctgaggc acgaacccga atggaagcct
      361 tgcacgccca atatccacag gcttcgctca ttgcccggca tctgtcacaa ctggatgaaa
      421 aactgggaaa tcccgatctg gccgaaaaag aaatcgagca ctatgccgaa cttgaacacc
      481 agagcgttga aagcctcgaa ctggtcgctg ccttccagaa ccgacaggct cgatttgacc
      541 acgaagccaa aacactggaa cgattgctca atcttgttcc aaaaacccaa aaagtctcga
      601 tgctgcaacg gctcatggcc cacgcgcgaa aacacaacct gacggcgtac ctcggtccac
      661 agtactacca gaacgtcctt gaacaggatc catccgtggt ctcagtcatt attgaactga
      721 ttgactggct ggcaggtgaa aaaaattacc cggaagccct gaaactggtc cggcagtaca
      781 aaccgcactt tccccaaaat accagttact ttgtcgtcac cgaagccaat ctcctggcca
      841 atctgggtcg cgaaaaagag gccgaaagcg ttttttcaac cgcactcgat gcacaatggt
      901 ctgaagccct tgcccagtca tttgacacgt ttctggatga acacgaccgg taccgcgctt
      961 tctattttga agtcaaagaa cagtttctgc agcacccaga gagcttttcc ctgctcgtca
     1021 aacgccttca ttatctggaa agacactata ccggtggctc agtcaaaaat gacattctcc
     1081 gccatgaaaa ggcccgtgct gaacgcaacc agccactgac gccagatgaa ctcctcatca
     1141 tcagccacta tttgattgag gaaaaccagc ctgatgaagc tgcccggtat ctctacacgg
     1201 tttgtttgca aacccaggtc aaacctggag acccaatccg agccaaagtc ctgtaccaac
     1261 tgtttgaaat cctgatggat gcccagaacg aacagctttc attgaccaac aacgacctgc
     1321 agttttatca agatattgcc acggcagatt cgcatccagg actgtttggc gccgtgctgt
     1381 cgcttatttt ttcagatagc gatgtgggcg ataaatttga agaaaaacaa accaacgcca
     1441 ctcgattctt taataaagct gcggcatttc ggattttttc agcctacaaa caggaaaacc
     1501 cgacttcgcc cgaactggca caaatgtacc tggatattgt tcggttgtat gccgccaccc
     1561 aggaacttca aattgccgac gcgacactgg ttgaatttga gcaacgccat caaaatgcat
     1621 cgcaatttcc agaagtcgcg ctcaaactgg cggatgccta tctggccagg gacaaccgcc
     1681 gcgaagaaca ggccgtttta cagcgggtgc ttgattactt cggcaaaaat cgaaaaccag
     1741 gcgaccagtt actgccggtt tctcctcaga ttggggtttc gactgaactc actggcccac
     1801 cgccctttca aacccagacc cagcctcaat ccccctccgg aattaacatc ccaaatacca
     1861 aagacagttc aacaactgag aattggagtt actcctatag ctcgtttaac aatcacctcg
     1921 gcctttcagc cgcagtccag ccccaaacca tcacgtatgc catcgttttg gatcggctgg
     1981 tttcttcgct ctttgtccag gagcggaggc aagacatttt gagcctgtat gccagcgaac
     2041 tgaaaaaata tccggacgaa cagggcctct atgaacaatg gttacaatgg cttgaccaga
     2101 ccaatctggc tgaagaacaa ctccgggtat atcgagaagc gatcacccat ttccccacaa
     2161 cgctctggca ccatcggctg gcacggtggc tgatccggac caaacgcgac cgtgaacttg
     2221 agactttttc ccgccagttg atctcgaccg taaatgacgc tgaaactgaa gcctatttgt
     2281 cgaattttat tgcttcacgg ctcaattcaa gcactgagtt tgatcggaac ctgtgccttg
     2341 ggctgtacca gatggcgcac aagcggttcc cgcataacct gatgtttgtc aatggattgc
     2401 tcagatacta tgaacagcgg aaagagtgga ccgaatggcg caagttgatg gcggaatatg
     2461 tttttgtatc cccagggatt cgccaggagt ttctatcaca tctggccagc cggaaagagc
     2521 ttcgatcctc agttgagcag gcacgcaaaa agtgtgcgac gacgacgaaa aatccgctgg
     2581 aactcctgcc ctacaaactg tttcaggccg atgcggccat ccggctttct gattttgaag
     2641 gcgcggtcga gacctatcgc gaactgattc gcctgtatcc aaacaatgcc gaatttaccc
     2701 tccgactggt ggcattgaat cgctcgtttg ggcaaaaaga ccgcaccgct ttaactgaag
     2761 cggccacaat tcaacatgcc tataccgaag ccaatcccgc catcagcggc cttcgcacca
     2821 cgtcaggcga actctatgcc gagttgggcg actatggtca ggccaaagct gaatgggaaa
     2881 aactgctcga ccttggccgt ggcgagcctg aaatctatct cgacacggcg acgctctact
     2941 gggactattt tcaatatgat gacgcgctgc acacaatttg gcggctccgg gaacaactcc
     3001 ataacgacag cctgtatgcc tatcaggccg gggccatcca cgaagccaaa caccagcgac
     3061 ccgaggcatt ggtggaatat gtcaaagcct taaaccagga aaccaccgat tatgctgacc
     3121 gccgcaaaac cagagcgcgg ttgaaggtct tgtatgaccg ccaggatgtt ccccaggcac
     3181 tggagcaggc actggcggaa agctatcgca cccaacctca acctgaatgg ctggtactcg
     3241 ggtatgccga atttttaaag gaagttggcc aggaagcgca ggcggtcaat cacctcaaaa
     3301 ccgaagtcac ccaaagccgg tcccaggaat ttctcacccg agcccgtagt tttttcagaa
     3361 atagcagtca cctggatggc gaagccacca cgctgcacca acttatcgca agtgccaaaa
     3421 gccatcggtt tgcgatttcg tttcacctgc aactggctga actgtacaaa aatcaccgcg
     3481 acctgcctgc cgcagaccgg gtgctccggc agctccggca acgatatcca ctcaattatg
     3541 gtgtgttaac cgaaaccgca gacatctact ggcggatggg gcaaagtaac caggcactcg
     3601 aaattctcaa gcagggacgt caacgcagta tcggaagatt tgcctatcgt tttgcccgta
     3661 aactggcggc gcgttatctc gacctgaacc agttcccgca ggccgaacag atactgcggc
     3721 agcttcatca ggaaaaccca ctcgattctg gtgtgttcca ggagctggcc cgaatgtatg
     3781 tcaaaaccag tcagccagcc gagttgggtg aggtttttca gcaaagcctc agcgccatcc
     3841 aaaaacagga tatcgaaatc caggaaattc gatatcaggt ggcggatgtg cgggaaaaaa
     3901 tgatcacggc gtttacgcag ttaaaagact atgacgccgc aattgaccag cacattgaaa
     3961 ttatcaaccg tgagcctgac gacgaagacc gactcaacgt tgcgctcagc tacgccaaac
     4021 ggtacggcgg cagcgaacgg ctgctggctt attaccagca aaccgcgaaa caggcgtata
     4081 aaaattatcg ctggtatctg gttctggcgc gaatttatga agccaaaaac gactggccca
     4141 gggccattga aaactaccgc acggcaattg ggaatcaacc ggaaaaggtt gatctctatg
     4201 cggccatcgc cgaagcccat ctcaagctca atcaattgaa cgaggccatc aaagccttgc
     4261 gcaaagccgc agaactcacc aacgacgatc cggtatacct caaaaagctg ataaacgtcc
     4321 tggaacaggc cggttatcac aaagaagccg ccgtggttcg acaaaaactc cccgaaccac
     4381 cgccaccaaa gcaacagact ttatcccagc agcttgccga agccgaacaa ctccgaacca
     4441 ccgacagggc caaagcgatt gaactctatc gaaaagtctt tgaaacctat gcgaccaacc
     4501 cgtacaaaaa tgaaatcaac atcggtgatc tgaccagtta cgtgcaaacg ctccattctg
     4561 aagaacctct ggataagatc gccgggcgac tgtgggacct gcgccagaaa ctcattacag
     4621 agcgcgaatc accaaccagt ctgcaagccg ggaccgctca ggaacgaatc aaat
//
LOCUS       DAOQDO010000153         4672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12225_length_4672_cov_2.769114, whole genome shotgun sequence.
ACCESSION   DAOQDO010000153 DAOQDO010000000
VERSION     DAOQDO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12225_length_4672_cov_2.769114"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>4672
                     /locus_tag="PKZ53_03565"
     CDS             62..>4672
                     /locus_tag="PKZ53_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ39543.1"
                     /translation="MGALLGQPLGPLAGRLNTSGNIDGIFGDARFNGPLGIGISGDQI
                     FMADTNNRSIRRVNLGTGQVRTVVALSDMIPAGITDWAPRAVAIDSNGDLYVTDAANH
                     TIWQITLNTTLDSVTASAKLLAGEIGQAGGQDGIGRTARFNSPQKITLTGNILSVVDR
                     NAGVRQIALPGGAVKTIRSTQGRTEVSSRFDPQQSPTLGTLKAVASDALGNLYVAEED
                     QVRVLTINGEQVVTSELAQSGTFVNPFAIAIADNSILILDSQKTQASLLRVAMAAPVI
                     NTVTPTQVGTGSSTEVTITGQNFIQETQVLIGQQLVQNLQLVSSGELKFTLPAQPTGG
                     TVPIAVRHRGGTAQSSINVSGTPQSTVNLSAFPTERSLRAGQSTTFTLSLGRTNATQA
                     IDLSAQVPAGLSATISPNPVAGDSSQVTVTSSPTVSPGRYNVQVTGTASGVTVSSVTV
                     SVVVEAQAQQPSVVLASTPGSRSVSSGQSTTYAIDLRRTNYQGVVNLSAGLVGTQTTG
                     IDLSFNPASTSSNTATLTARTSAAVPRGEYTIRVNASFLGGSAVPIQVLLVVNPDPAQ
                     VTLSLAQNSQTVNAGDAAPYTVTGTTSNFSGSVNLSLTGNIPFQATISLSPSPMPVPG
                     SSTLTIRTATTTPPGTYAMQVVGSASGVSIAPVPFTLTVRSAQGQSTVAMAISPATQT
                     AASGQNAEYALTLRRTNFTGAVDIRVTATGYDPFGGTITGDTATITVWNTTPGTYTFT
                     VTGTAQGATVTNTVTFTLVVSQQSGGPTVNISASPGSMTVNRGQSANYAINLSRNGFT
                     GPVTLQTFIRSTTIPPGITLNYSQNPVTGATTTLTLFTTTETPGGTYNFYLEGTATGA
                     TVTRSADFTLVVNSTVTPTVNLGVSPTTQTVTQGQGSTFTVALNRTNFTGSVSLQVFI
                     QAQVTEPTITFQFAQNPTTGNSTSLSMSTTSATPPGVYTFFVRGTASGANVIDSPTFT
                     VVVNSGNGGSENGCAVPFSIDIGQTIDRTLDTSCVYQGTQRNIDVYRFNGTAGQQVTI
                     TLSSSAFDAYLYLFDSNRNTLASDDDTNGTDSQIVFTLPYSGSYQLDVTSYFENSTGP
                     YRLVLAGAGGSCNYSISPTSRSHGAGTETGSVSVTTTSGCNWTATSNSTFVTITSGTS
                     GSGSGSVSYRVDSNTGSTTRTGTLTIAGQTFTITQAAGGGGGEDGCVTPFTVSIGQTI
                     NRTLDTSCAYQNTTQYLDTYRFSGTQGQSVTITLSSSAFDTFVYLLDANRNTLASNDD
                     FSGTNSQVSFTLPSTGLFGIDVTSAVSGATGPYTLVISAGGGTNEDGCVTPYTINFGQ
                     TVRRTLDGSCVYSNNENRFVDVYRFNGSAGQQVTITHSSTAFDAYLYLYMPGTLLAED
                     DDSAGGTNARIVYTLPSSGTYFIDATAFSDNATGDYSLSLSGGSLNRLDRVPQPTDLE
                     FGETVVVKSDSTGVTFSQKPETYQFLGQPGQQVMVTLTSPAPNWQVHLKDPSGNRLVE
                     NAMVGNPTSHLTQTLQSGGFYEIEVSSTGEASSGGYTLTLARI"
BASE COUNT         1066 a         1316 c         1244 g         1046 t
ORIGIN      
        1 ttttttctct tacgatactt cagaagcact gcatgtttca gaccgggaaa aacacatttt
       61 aatgggtgct ttgcttgggc aaccgcttgg accgctggcc ggaagactca acacgagcgg
      121 caatattgat ggcatttttg gtgatgcccg gtttaatggc ccgctcggaa ttggaatttc
      181 cggtgaccag atttttatgg ctgacaccaa caaccggagc attcgccggg tgaaccttgg
      241 cacgggtcag gtccgaaccg tggtggcttt gagcgatatg attccggctg gcatcactga
      301 ttgggcaccg cgagctgtgg cgattgattc aaatggtgac ctgtatgtaa cggatgccgc
      361 caatcacacc atctggcaaa ttacgctgaa cacaacactt gattcagtga cggcttccgc
      421 caaattgctg gctggtgaaa ttggtcaggc tggtggtcag gatggcattg gtcggacggc
      481 acggtttaac agtccacaaa aaatcacgct gacaggaaat attctcagcg tggtggatcg
      541 caatgccggt gtgcgccaga ttgccctgcc gggtggcgcc gtgaaaacaa tccgctcaac
      601 ccaggggcga accgaggtca gcagtcgctt tgacccccaa caatctccaa cactgggtac
      661 cttgaaagcc gtggcgtctg atgcgctggg aaacctctat gtcgctgaag aagatcaggt
      721 tcgggtgttg acgatcaatg gcgaacaggt ggtgacctcc gaactggccc aatcgggaac
      781 atttgtcaat ccgtttgcca ttgcgattgc cgacaattcg atcttgattt tggacagcca
      841 gaaaacccag gcgtcgttgt tgcgtgtggc gatggcggcg ccggtcatca ataccgtgac
      901 gccaacccag gttggtactg gcagcagcac tgaagttact atcacaggcc agaattttat
      961 ccaggaaact caggttttga tcgggcagca actggttcag aatctgcagc tcgtcagttc
     1021 tggggaactc aaatttacac ttccggccca gcccaccggg ggaacggtac cgattgcggt
     1081 tcgccaccgg ggcggcaccg cccaaagctc gatcaatgtc agtgggactc cgcaatcaac
     1141 cgtcaatttg tcggccttcc caaccgagcg gagtttgaga gccggccaaa gtaccacctt
     1201 taccctttca ctgggccgaa ccaatgccac ccaggcaatt gacctgtccg cccaggtgcc
     1261 agccgggctt tcagccacca tcagtccaaa tccggtggct ggtgattcat cacaggtgac
     1321 agtgacctcc agcccaaccg tttcgccagg acggtacaat gtccaggtga ctggaacggc
     1381 gagtggtgtc acggtcagtt cagtcacggt atcagtcgtg gtcgaagccc aggcccagca
     1441 accatcagtt gtcctggcct caacaccggg ttcgcggtca gtctcttccg ggcaatcaac
     1501 gacctatgcg attgatctca ggcggaccaa ctatcaaggc gttgtgaatt tgtcagccgg
     1561 attggtcggg acacagacca ctggtattga tctgagcttt aacccggctt caaccagcag
     1621 taacaccgcg acgttgacgg cgcgcaccag tgctgctgtg ccgcgtggcg aatacaccat
     1681 tcgggtcaat gcttcatttt tgggtggttc ggcagtaccg attcaagttc tgttagtggt
     1741 caatccagat ccggcgcagg tgacgctttc gctggctcag aattcgcaaa cggttaatgc
     1801 cggtgatgcg gcgccctaca ccgtcaccgg taccacctcg aatttttccg gttcggtcaa
     1861 tttgtcactg actggaaaca ttccatttca agccacgatc agtctgagcc cgtcgccgat
     1921 gccggtgccg ggaagttcaa ccctgaccat tcgaacggcc acgaccacac cgcccggcac
     1981 ctacgccatg caggtggttg gcagtgccag cggggtatcc attgcgcccg tgcccttcac
     2041 gctgacggtt cggagcgccc aggggcaatc aaccgtggca atggccatca gcccggcgac
     2101 gcagaccgcg gcatccgggc aaaatgctga gtatgcgttg acgctccggc gcaccaactt
     2161 taccggggcg gtggatattc gggtgaccgc cacgggatat gatccatttg gtgggacaat
     2221 tacgggcgac accgcgacga tcacggtttg gaacacaacg cctggaacct atacttttac
     2281 ggtgaccggg acggctcagg gagcgacggt gaccaatacc gtcacgttta ccctggtggt
     2341 gagtcaacaa tcaggcgggc caacggtcaa tatttccgcc agtccaggtt cgatgaccgt
     2401 caatcgtggg caaagtgcaa attatgccat caatttatcg cgcaatggat ttaccgggcc
     2461 agtcacgctc cagaccttta tccgctcaac cacgattcca cctggaatca cgctcaacta
     2521 cagccagaac cccgtaaccg gggccacaac caccctgacg ctgtttacca cgactgaaac
     2581 gccaggcggc acctacaatt tctatcttga aggcacagcg actggggcaa ccgtgaccag
     2641 atcggctgat tttaccctgg tggtcaattc aactgttact ccaaccgtca atttgggagt
     2701 gagccccacg acccagacgg tgactcaagg gcaggggagt accttcactg tggcgctcaa
     2761 tcgaaccaat ttcaccgggt ctgtgtcact gcaggtcttt atccaggccc aggtcactga
     2821 gccaaccatt accttccagt ttgcccaaaa tccgacgacc ggaaattcga cttcactgag
     2881 tatgtccacg accagcgcga caccgccggg agtctacaca ttttttgtcc gagggacggc
     2941 gagcggtgcc aatgtcattg attcaccgac ctttaccgtg gtggtcaatt cgggcaatgg
     3001 cggcagcgaa aacgggtgtg ccgtgccatt ttcaatcgac attgggcaaa cgattgatcg
     3061 cacgctcgac accagttgtg tctatcaggg gacacaacgc aacattgacg tgtaccgatt
     3121 taatggcact gctggtcagc aggtgacgat tacgctttcg tcatcagcct ttgatgccta
     3181 cctgtatctg tttgacagta atcgcaatac actggcttca gacgatgaca ccaatgggac
     3241 ggattcacaa attgtcttta cgctgcccta tagcgggagc tatcagcttg atgtgacttc
     3301 gtactttgaa aattcaaccg gaccatatcg gctggttctg gccggagccg gcggcagttg
     3361 caactactcg atttccccaa ccagccgctc gcatggcgcc ggaactgaaa cgggttctgt
     3421 ttcggtcacg acgacctcgg gctgtaactg gacggccacc agcaatagca catttgtgac
     3481 cattacctcc ggaaccagcg gcagcggcag cggcagcgtg tcgtaccggg ttgacagcaa
     3541 tactggcagc acaacccgaa ctggtaccct caccattgcc gggcaaacat ttacgattac
     3601 ccaggcggca ggtggcggtg gtggagaaga cggctgcgtg acgccattta ccgtcagtat
     3661 tggccagaca atcaaccgga ccctcgacac cagttgtgcc tatcaaaaca caacccagta
     3721 tctggatacc tatcggttct cgggcactca ggggcagtcg gtgacgatta ccctgagttc
     3781 atcggccttt gacacgtttg tctatttgct ggatgccaac cgcaacacac tggcttcaaa
     3841 cgatgatttc agcggcacca actcgcaggt cagtttcacg ctcccctcaa ccggtctgtt
     3901 tggaattgac gtaacctcag ccgtttctgg cgccaccggg ccctataccc tggtcatcag
     3961 cgccggaggt ggaacaaatg aagacgggtg cgtgacgcca tacaccatta attttggcca
     4021 gaccgtgcgt cggacgctcg acggaagttg tgtctattcc aacaatgaaa atcggtttgt
     4081 ggatgtgtat cggtttaacg gttcggctgg ccagcaggtg acgattacgc actcctcaac
     4141 cgcctttgac gcctatctgt acctctacat gcccggcacc ttactggctg aagatgatga
     4201 ttcggctggt ggcaccaacg cccggattgt ctataccttg ccatccagtg gaacatattt
     4261 cattgatgcg acagctttta gtgataacgc aaccggagat tatagcctgt cgttgtctgg
     4321 cggtagcctc aatcggctcg acagagtgcc acaacccact gatcttgaat ttggtgaaac
     4381 cgtggtggtc aaatccgact cgacaggtgt cacgtttagc cagaaaccag aaacctatca
     4441 gtttttaggt cagccgggac agcaggtgat ggtcactctc acttcacccg caccaaactg
     4501 gcaggtccat ttgaaagatc cgtctggaaa tcggctggtg gaaaatgcta tggtgggaaa
     4561 tcccacatct catctcaccc aaacacttca gtccggtgga ttctacgaaa ttgaagtaag
     4621 ttcgactggt gaagcgtcgt caggtggata tacactgaca ctcgcacgta tc
//
LOCUS       DAOQDO010000154         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12253_length_4667_cov_2.772333, whole genome shotgun sequence.
ACCESSION   DAOQDO010000154 DAOQDO010000000
VERSION     DAOQDO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12253_length_4667_cov_2.772333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKZ53_03570"
     CDS             <1..469
                     /locus_tag="PKZ53_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39544.1"
                     /translation="GRSAAGCARGSDQVGDQTLYTQVFETLFSSVQSDSAACSRLMAF
                     LDEHDQLPVIKAMAQFLLESFQTQPTPTAARQCLFFSARTGSAALYSEVVDQLMVHWR
                     RGNLTGIEPANLAALIESQFWLLPVQARSSGDGHVLKRVLALMRRELTAYEPH"
     gene            528..2669
                     /locus_tag="PKZ53_03575"
     CDS             528..2669
                     /locus_tag="PKZ53_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ39545.1"
                     /translation="MELIAVAGIVFALIVFLMVFNSFFVSVGATEIAIKERRYFGEKM
                     PPGRVVATAGEVGIQAEVLKPGLHFIKWPMERIYSKVPLVSVGSDEMGIVEAIDGEPL
                     PPGRIFAPDRAQNAHNNFQDPIAFIKQGGVKGIQLRTLPPGLWPIHPYLFRVSIAKST
                     MIPQGKVGMVIAADGSPLDPGRLLGKAIEGHRNFQEAEQFIAANGQKGSQVEVLTPGT
                     YRIHTESISFAGEKKPGIFTVRLCDATFITENQVGLVEALDGSPLDPRDYVAQPVEGH
                     DNFQDADEFLARGGQRGPQKDILLPGIYYINPLLFKVIPEKAGEVKPGEVAVIVSNVG
                     KDPSEEIRRAMALKIRARLEQEEQEQARLMAAKLTGTAKKATAAELAAEFRVNDPADT
                     RLDQGAHEAYVVPEGYRGIRETVVGPGRYYINTLAITPIIIPTTNQTVEWTAEDIDNT
                     FNPFEVISKDGFSMQLEVRVVFRVKPEDAPFMVAKIGSIDRLIKNVMHPLIDSIFRNQ
                     ASESSAMAYLQNRHEEQERAEARVRAHLLKYHVDVVNVLICHIHLPEELMKTQTEKIL
                     AEQRQNMFFAQREAEDKRIQLEKTKAHADNQRDLMAATVGVEIATKRAEQRKAEGEGE
                     AHFISATGRAEAEKVRLMGEAQGVAYAEQVKAMGPQGVALIETLKVIGEKGVRITPDV
                     MATGGQSEGGGGLGTLLLLNLFRDRLGDGGK"
     gene            complement(2940..>4667)
                     /locus_tag="PKZ53_03580"
     CDS             complement(2940..>4667)
                     /locus_tag="PKZ53_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39546.1"
                     /translation="TGATASGSNTIVTGTLNSSANTAFRIEFFSNPTCDASGNGEGQI
                     FLGFTNVTTIGNNASFSTTLPVATTAGQVVTAIATDAAGNSSEFSACQVVCGPITATV
                     SGSGTSCADTPGTFPVTVTVSGGVGPYTVTLTNGGGTQMGSSPLTFMVSPSSTTTYTL
                     QSGSDVNGCGLTGSGSATMTVNSVATVDAGLPQTVCDNQPAVTLAGVIGGSGTAGTWS
                     GGGGTFTPDNRTLNATYTPTPAEVSSGSVTLTLTADDPPGPCGSVSDTVTITYDVCGL
                     ERSILMVADTSNNRIQQFDGNNWSVIGTGMVGSGDGQFRLPEAVAFDASGQRIYVADT
                     GNNRIQWSTDGGASWATFANVGAGLNQVRAPQGLAYDPQGNLYVADTGNGRVLRFDGG
                     IPGFGVVIATNGAGSGQVGSPRGLTIDLSFRLFVADESNSRILRINNASTVTSSTTGV
                     IVASRGVGLNQVQNPQGVAIDTTGTLYIADTGNSRILRFLNANPANSTSMALAGSSLG
                     QVNRAEGVTVSLFATGPFAGMPFVIVGDTGNHRIQGRFVSTGGWALVGTPNGNGTAVG
                     RFRSPGKIR"
BASE COUNT         1125 a         1289 c         1249 g         1004 t
ORIGIN      
        1 aggccgatct gcggctggat gtgcgcgagg aagcgatcaa gtcggcgatc aaacgctgta
       61 tacacaagtg tttgagactc ttttttcctc ggtccaatca gactcagccg cgtgctcacg
      121 cctgatggcc ttccttgacg aacacgacca gttgcccgtc atcaaggcga tggctcagtt
      181 tctgcttgaa tcgtttcaaa ctcagccaac accaaccgcc gcccggcaat gcctgttctt
      241 ttcggctcgc accggctcgg cagcccttta ttccgaagtg gttgatcagc tcatggtgca
      301 ctggcgacgt ggcaacctga ctgggatcga gccggcaaac ctggcggctc tgatcgaaag
      361 ccagttctgg ctgctgcccg ttcaggctcg ctcatccgga gatggtcatg tgttgaaacg
      421 ggttctggct ttgatgcgac gtgaactgac agcctacgaa cctcactaat gttcagtgaa
      481 gcctttacca cggttggttc tttgtcctca gtacagaaag gttttgtatg gaactcattg
      541 ctgttgcagg aatcgttttt gcgttgattg tctttttgat ggtctttaat tcgttttttg
      601 tcagcgttgg cgcaacggaa attgcgatta aggaacgtcg ttactttggt gaaaaaatgc
      661 ctcctggacg ggtcgtggca acggctggcg aagttggcat ccaggctgaa gtcctcaaac
      721 ccggattaca tttcatcaaa tggccaatgg agcgaattta cagcaaagtc ccactggtct
      781 cggttggctc agacgaaatg ggtattgtcg aagccattga cggcgagcca ttgccaccag
      841 gccgaatttt tgccccagac cgggcgcaaa atgctcataa caattttcaa gacccgattg
      901 cctttatcaa acagggcggc gtgaagggaa tccagttgcg aacactcccg ccggggctgt
      961 ggcccatcca tccatatctg tttcgggttt caattgccaa atcaacgatg attccacagg
     1021 gcaaagtcgg catggtcatt gccgccgacg gcagtccgct tgatcctggc cgcctgcttg
     1081 gcaaagcgat tgaaggtcac cgcaactttc aggaagctga acagtttatc gcggccaatg
     1141 gccagaaagg gtcgcaggtc gaagtcctga cgccaggaac ctatcgcatt cacactgagt
     1201 ccatttcgtt tgcgggtgag aaaaaaccgg gaatttttac ggtccggctc tgtgatgcca
     1261 cattcattac cgaaaatcag gtcggactgg tcgaagccct cgacggctcg ccgcttgatc
     1321 cacgtgatta tgtcgcccaa ccagtcgaag gccacgacaa ctttcaggat gccgacgaat
     1381 tcctcgcccg tggcggccag cgtggcccgc agaaagacat tctgctgcct gggatttatt
     1441 acatcaaccc gctgctcttt aaggtcattc cggaaaaggc cggtgaagtc aaacccggcg
     1501 aagtcgcggt gattgtctca aacgtcggga aagatccatc cgaagaaatc cgccgggcaa
     1561 tggcgctcaa gattcgggcc cgccttgagc aagaagaaca ggaacaggcc agactgatgg
     1621 ccgctaaact caccggaacc gcgaaaaaag cgacagccgc agaactggcc gcagaatttc
     1681 gggtcaatga cccggcagat acgcggcttg atcagggtgc gcacgaagcc tatgtggtgc
     1741 ccgaaggcta tcgcggtatt cgcgaaacag tggttggccc aggccgctat tacatcaaca
     1801 cgctggccat caccccgatc atcattccca ctaccaacca gacagttgag tggacggctg
     1861 aagacattga taacacgttc aacccgtttg aagtcatttc aaaagacggg ttttccatgc
     1921 aactcgaagt ccgcgttgtg tttcgcgtca aacctgaaga tgcaccgttt atggtcgcca
     1981 agatcggctc gattgaccgg ttgatcaaaa atgtcatgca tccgctgatt gattcaattt
     2041 tccgcaatca ggcttcggaa tcatccgcga tggcctattt gcaaaatcgt cacgaagaac
     2101 aggaacgcgc tgaagcccgt gtccgggcac atttgctcaa atatcacgtg gatgtggtca
     2161 atgtgctgat ctgccatatc cacctgcctg aagagttgat gaaaacccag accgaaaaaa
     2221 tcctggccga acagcgtcag aatatgttct ttgcccaacg tgaggcagaa gacaagcgca
     2281 ttcagcttga aaaaaccaaa gcccatgctg acaaccagcg cgatctgatg gcggccacgg
     2341 tcggcgttga aatcgccacc aaacgcgccg aacaacgcaa agctgagggc gaaggcgaag
     2401 cgcacttcat ttcggcaacc ggtcgggctg aagcagagaa agttcgattg atgggtgaag
     2461 cccagggcgt tgcctatgcc gaacaggtca aagcgatggg cccacagggt gtggcgttga
     2521 ttgaaacctt gaaagtgatt ggtgaaaaag gagttcgcat cacacctgac gtgatggcca
     2581 caggcggcca aagcgaaggt ggtggcgggc ttgggacgtt gctcctgctc aacctgttcc
     2641 gggaccgcct gggtgatggc gggaaatagg ggttagggtt ttcggaaggg atgagggatg
     2701 aaagatgagg gatgaaaaaa atcaccctct caccttgtca ttcggtcatc ttgtcagggt
     2761 tccagggttt gaaagaccgg ggttaggggc ataagcgcca ggataaggat tttagcccgg
     2821 cacgggcgtc gtacaatagc cgtggtgcgc agcccacgga aacaaaacca accattgatt
     2881 caaaaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc ctgctttcct
     2941 caccgaatct tgcccgggct gcggaaccgt ccaacagcgg tgccgttgcc attgggcgtt
     3001 ccaaccagtg cccagccgcc ggttgacaca aaacgaccct ggatccggtg atttccggta
     3061 tcgccaacga tcacaaatgg cattccggca aacggtccgg tcgcaaacag gctgacggtt
     3121 acgccttcag cccggttgac ttgaccgagt gaggaaccag ccaacgccat gctggttgaa
     3181 ttggccggat tggcgtttag aaatcgtaaa atacgcgagt tgcctgtgtc ggcaatatac
     3241 aaagtcccag ttgtatcaat cgcaacccct tgtggattct ggacttgatt gagcccaaca
     3301 ccccgcgaag caacaatcac cccggtggta ctgctcgtga cggtgctggc attgttaatg
     3361 cgcaaaatcc ggctatttga ttcatccgcc acaaacaacc tgaaactcaa atcaatggtc
     3421 aatccacgtg ggctgccaac ctgaccgctc ccagcgccgt tggtggcaat cacgacacca
     3481 aatccaggaa tccccccgtc aaatcgaagc acccgcccgt tgccagtatc agcaacatag
     3541 agattgcctt gtggatcgta agccagccct tgcggtgctc gcacctgatt caagccagca
     3601 cccacattgg caaaagttgc ccacgacgca ccgccatcag ttgaccactg aatgcggttg
     3661 ttcccagtat cagccacgta aatccgctgg ccactggcat caaatgccac cgcctctggc
     3721 aatcggaact gaccatcacc cgaaccaacc attccggttc caatcacaga ccagttgttg
     3781 ccgtcaaatt gctggattcg attgtttgaa gtatcggcga ccatgagaat agaccgctca
     3841 aggccgcata cgtcataagt aattgttacc gtatcgctta ccgaaccaca gggcccaggc
     3901 ggatcatccg ccgtcaacgt cagtgtcacg gatccagagg aaacttcagc cggcgtcgga
     3961 gtataggtgg cgttgagcgt gcgattgtca ggggtgaacg tgccgccacc gccgctccag
     4021 gtaccagccg ttccactgcc accaatgacc cctgcgagcg tgacggcagg ttgattgtca
     4081 cataccgttt gcggcaaacc agcatccaca gtcgccactg agttgaccgt catggtggcg
     4141 ctcccgctcc cggtcaaccc acaaccattg acgtcgcttc cggattggag ggtgtaagtt
     4201 gttgtggatg aaggcgaaac cataaatgtc aaaggactgc tccccatctg ggtgccgccg
     4261 ccattggtga gcgtaaccgt gtatgggcca acccctcctg acacggttac ggtcacagga
     4321 aacgttcccg gagtatcagc gcaactggtg ccgctcccgc tgaccgttgc ggtaatggga
     4381 ccgcaaacaa cctgacaggc tgaaaattca gatgagtttc cagcagcatc cgtcgcaatc
     4441 gccgtcacaa cctgtccagc agtggttgca acgggcaatg tcgtactgaa acttgcattg
     4501 ttcccgatag ttgtaacatt ggtaaaccca agaaagatct gaccttcccc atttccagaa
     4561 gcatcgcagg ttgggtttga gaaaaattcg attcggaaag ctgtgtttgc tgagctgttg
     4621 agcgttccgg tgacgatggt attgctccca gatgccgtgg ccccggt
//
LOCUS       DAOQDO010000155         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12259_length_4665_cov_2.994577, whole genome shotgun sequence.
ACCESSION   DAOQDO010000155 DAOQDO010000000
VERSION     DAOQDO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_4665_cov_2.994577"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..>4665
                     /locus_tag="PKZ53_03585"
     CDS             163..>4665
                     /locus_tag="PKZ53_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNJ39547.1"
                     /translation="MKHSFWAVWSFILLFGCGLGIALIHPGTACSQISQQSGQPTRGL
                     RFRLHSESAPPVSAITSRAKSPAIWLDATTSTNLLRQVPNLLQPPSLNLPRFRFPDKI
                     MIPPQSQVTKTTIFSPQASLAVPETPGAGPLKVLRYMPQGEVEQAVALSVTFSQPMVA
                     LSSLHELSKVEIPVRLSPQPPGKWRWIDTSTVLFEPDGGQFPKATDYKVAILSEARSL
                     AGNQLGQPVEWEFSTPGLKIIRSYPPQPEPQNSTDTRYFSVFESRQPEIFIELNQQIQ
                     PRDLIQTLFIMTSTTQIAVELVDPAGLKLDFVLESFIKQAKPGCWMLFRPVQPLPDNA
                     LISISILPGRHSSEGPAPPQPTQTLRFRSIQPLEVYEGINDDSNWSGITNKVIQFSNA
                     LDNHSFRPEMVQVEPELEGKTVTLKDRILEVGGKFRRRTTYSITLSPEICDIYGQHLG
                     KSKTMQITTQRASTELWLPAQDLLTLDPYGPPEFPVFTVNVKTFSLQLYQVEPSDWYA
                     YLDLGTNPAAYRGQVIPPGLKKVDRQVVPKNDLDRLVETRIDLREALSGKVGHVLAVI
                     KWSQYPTDPNYEKTHFLWIQITNLAADVTNEFYRLSVWVNRLDTGKPVPKAVVTLLPE
                     GKSAQVDHHGQVRFQASTKKINKAGTILVQKGPDSVIVIDSVNFHVGNQNWGGGQSIP
                     GPYFTWYTVDDRKLYRPGESVHIKGWVRHLLPSAKGNMGQVRPFAKQVKYRVLDAFQT
                     ELATGTIHLSRLGGFECQFTLPDSCRLGVGQVVFELVTRYKKEAYDTKQTTHFFEIEE
                     FRRPEYEVQVDVADGQHVIGTQASVQASVHYFTGNTLPNSQVNWTVTAISQPFTPPTW
                     YDYQFGQWQSWHTAEEFNRKYTASETCSGQVDARGQHQLKIDLDAVEPVVPMLVKTEA
                     SVTDINRTNISGRAEFLVHPATLYVGIRSQWNFAERGQDYPVELIVTDPDGKLQPDQN
                     IELSLFRLEQFSFEGESEVLDRPIAQAQVVSANHPVVWKAPATESGPYRVRATIRDDH
                     NRMNLSEITFLQLGDLSQFNQFARFIDPERIELTANQSSYQPGETAEIKVESPFAPAE
                     GLLTISNSGVLSSRRFTLNSRSEVLKIPITKSNVPILSIVIDLVGTAPRYEGNQGSVV
                     PPRPAFATKKLELPVKPTDQTLSVAVTPISTEVQPGKETAVDLEIKDAHGKPVRESEC
                     ALIVVDEAVWSLTKNHISHPLEIFYQYNSPFVFFFHSRQTVALYPFGKLGQSQPPQGS
                     ENPAYEAWKLVLWDGLKLRKIDPERWNNYPPPFSPPREASQVSTAPFPQIGSFTPQVV
                     NSPSTPVTLRSNFSPLAAFIGSIITDAQGRARATFKMPDNVTRYHIIALAAAGENQFG
                     KGESTITARLPLMVRPAAPRFVNQGDRFELPVTIHNQSDFDAVVNIVIQSHELAFPQG
                     IGQQIFVPSNDRVELRFPAEANQVGTARVRIATFSTLMTDAAEIEIPVYSPVSSESLC
                     TYGELET"
BASE COUNT         1184 a         1272 c         1112 g         1097 t
ORIGIN      
        1 agcgaccacg ccaggaaaat tcctggtgcc accggcgaaa gctgaagaaa tgtacgcccc
       61 cgaaaccttt ggccgggggc agaccgatgt cgtcgtgatc ggcgaataat ttcagaaagc
      121 ggcgtcaagc cgccgcactc caaattttgc ctctttttgc ctatgaaaca ttcattttgg
      181 gcagtgtgga gttttatatt gctgttcggt tgtggattgg gaatagccct gatccatcct
      241 ggaacagcct gttcgcaaat cagtcagcaa tccggtcagc caaccagggg actgcgcttt
      301 cgactgcatt ctgagtcagc accaccagtg tctgccatca catccagagc gaagtcaccg
      361 gcaatctggt tggatgcaac caccagcacc aacctgctcc ggcaagtccc caatctgttg
      421 caaccgccgt cactgaatct gcctcgcttc cgatttcctg acaaaatcat gatccctccc
      481 cagagtcagg tgacaaaaac gacgatcttt agcccccagg cttccctggc ggtacctgaa
      541 acaccgggcg ctggtccact caaagtgctg cgatatatgc cacagggcga ggtcgaacag
      601 gccgtggcac tcagcgttac gttctctcaa ccaatggtgg cgctgtcttc gctccacgaa
      661 ctctcaaagg ttgagatacc agtcaggctt tctccccagc ctccgggcaa atggcggtgg
      721 atcgacacca gtaccgtact ttttgaacca gacgggggac aatttccaaa ggcgacagat
      781 tacaaggttg cgattttgag tgaagccagg tcactggctg gaaatcagct tgggcagccg
      841 gtggagtggg aattctccac cccaggactg aaaatcattc gttcataccc tccccaacct
      901 gaacctcaaa acagcacaga tacccggtat ttcagtgtgt ttgaaagccg acaacctgag
      961 attttcattg agttgaacca gcaaattcag ccccgtgatt tgattcaaac gctctttatc
     1021 atgacctcaa ccacacagat tgcggtcgaa ctggttgatc cggctgggct caaactggat
     1081 ttcgttcttg aatcgttcat caagcaggcc aaacctggat gctggatgct cttccgtccc
     1141 gtacaaccat tgccagacaa tgcgttgata tcaatttcta tcctgcctgg ccggcattcc
     1201 agtgaaggtc cggcgccacc tcaaccgaca caaaccctga gatttcgtag cattcaacca
     1261 ctggaggtat atgaaggaat caatgatgat tcaaactggt caggaatcac taataaggtc
     1321 attcagtttt caaacgcgct ggataaccat tcgtttcgac cagaaatggt ccaggtggaa
     1381 cctgagctgg aaggaaaaac cgtcacatta aaggacagaa tccttgaggt aggtgggaaa
     1441 tttcgtcgtc gcacgaccta tagcatcacg ctgtccccag aaatttgtga tatttacggc
     1501 cagcatctgg gtaaatccaa aacaatgcag atcaccaccc aacgagcgtc aactgagctt
     1561 tggctaccag cacaagacct acttaccctt gatccctatg gcccgccaga attcccggtg
     1621 tttacggtca acgtcaaaac cttttcactc caactttacc aggtcgaacc atcggactgg
     1681 tatgcctacc ttgatttggg tacaaacccg gctgcttacc gggggcaggt catccctccg
     1741 ggtttgaaaa aagtggatcg tcaggttgtt cccaaaaatg acctggatcg gctggttgag
     1801 accaggattg atttacgtga ggcactttca ggaaaagttg gccatgtgct ggctgtcatc
     1861 aaatggagcc aatatcccac ggatcccaac tatgaaaaaa cccattttct gtggattcaa
     1921 atcaccaatc tggccgccga tgtgaccaat gaattttacc gattgagcgt ctgggtcaat
     1981 cggcttgata ccggaaaacc agttccaaag gcagttgtga ccctgttacc tgaagggaaa
     2041 tcggctcaag ttgaccacca cggacaggtt cgctttcaag cctccaccaa aaaaattaac
     2101 aaggctggaa caattctggt tcagaaaggt cctgatagcg tcattgtgat tgatagcgtc
     2161 aattttcatg ttggaaatca aaactgggga gggggtcaaa gcattcccgg cccttacttt
     2221 acctggtaca ccgttgatga tcggaaactg taccgccccg gagagagcgt ccatatcaaa
     2281 ggctgggttc gccatctgct accttctgcc aaaggaaata tgggacaggt ccgtccgttt
     2341 gccaaacagg taaagtatcg agtgcttgac gcattccaaa ccgaactggc gacgggaacc
     2401 atccaccttt cccggttagg aggatttgag tgtcagttta cccttccgga ttcctgtcgg
     2461 ctgggcgtcg ggcaggttgt ttttgagctg gtcacccgat acaaaaagga agcatatgat
     2521 accaaacaaa ccactcattt ctttgaaatc gaagagtttc gccggccaga atatgaagta
     2581 caggttgatg ttgctgatgg tcagcacgtg attggcaccc aggccagcgt ccaggccagc
     2641 gttcattatt ttaccgggaa tacccttcca aactcacaag tcaactggac ggtcacggcc
     2701 atcagtcagc cgtttacacc tccaacctgg tacgactacc agtttgggca gtggcaatcc
     2761 tggcataccg ctgaagaatt caaccggaaa tacaccgcat ccgaaacctg ctctggtcag
     2821 gtggatgcga gaggtcagca ccagctcaag attgatcttg acgccgttga gccggtcgtt
     2881 ccgatgctgg tcaagaccga agcttcggtc acagatatca atcgaaccaa catttcaggc
     2941 agagctgagt ttctggttca tccggcaact ctgtatgttg gaatacgaag tcagtggaac
     3001 tttgccgaac gcggccagga ctatccggtt gaactcatcg taaccgaccc ggatggaaag
     3061 ctccagcctg atcagaacat cgaactcagc ctgtttcggc ttgaacaatt ctcttttgaa
     3121 ggtgaatcag aagtgctgga tcgtccaatt gcccaggcac aggtcgtttc ggccaatcat
     3181 ccggttgtct ggaaggcacc ggccacggaa agcggcccgt atcgggtgcg agccaccatc
     3241 cgggatgatc acaaccggat gaacctgagt gaaattacat tcctgcaact cggagacttg
     3301 agccagttta accagtttgc tcgatttatt gacccggaaa gaattgagtt aaccgccaat
     3361 cagtcatcct atcagcctgg tgaaacggct gaaatcaagg tcgagtcgcc atttgcgcca
     3421 gccgaaggac tcctgaccat ctcaaatagt ggagttcttt cttcccgtcg gtttactctg
     3481 aattcacgct cggaagtcct caaaattccg attacaaagt cgaatgtgcc aatcctcagt
     3541 attgtgattg atctggtcgg caccgcgcca cggtatgagg ggaaccaggg gtcggttgtt
     3601 cccccgcgcc cggcctttgc cactaaaaag cttgaactcc cagtaaaacc caccgatcaa
     3661 actctctcag ttgccgtgac cccaatttcg accgaagtcc agccaggaaa agaaacagcc
     3721 gtggaccttg aaatcaaaga tgcccacggc aagcccgtca gagaaagcga atgtgcgttg
     3781 atcgtggtgg atgaagccgt ctggtcgttg accaaaaatc acatttcaca tccgctcgaa
     3841 attttttacc aatacaattc cccgtttgtc tttttctttc attcacgtca gaccgtcgcg
     3901 ctatatccct ttggaaagtt ggggcaatca cagccacccc agggttcaga aaacccggcg
     3961 tacgaagcct ggaaactggt attgtgggat ggtttgaagc ttcgcaaaat tgaccccgag
     4021 cgatggaaca actatccgcc accattttcc cctccgcgcg aagcctctca agtatccacc
     4081 gctccatttc cccaaattgg cagctttact cctcaggttg tgaactcacc gtcaacccca
     4141 gtcacacttc gaagcaactt ttccccactt gcggctttta ttgggtcaat catcaccgat
     4201 gcccagggcc gggccagagc caccttcaaa atgccagata acgtcacccg ctatcacatc
     4261 attgccctgg cggcagccgg agaaaatcaa tttgggaaag gtgaatcaac catcacggct
     4321 cgattacctt tgatggtgcg tcctgccgcg ccccggtttg taaaccaggg agaccgattt
     4381 gaattgccag tgacgattca taatcaatct gattttgatg ctgtggtgaa tatcgttatc
     4441 cagtcacacg aacttgcctt tccacaaggc atcggccagc agattttcgt tccgtccaat
     4501 gaccgggtgg aactccggtt tccagccgaa gccaaccagg tgggaaccgc ccgggtccgc
     4561 attgccacct tttcaacgtt gatgaccgat gccgccgaaa ttgaaattcc agtgtattca
     4621 ccagtttcga gcgaatcatt gtgtacctat ggggagcttg aaacc
//
LOCUS       DAOQDO010000156         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12273_length_4661_cov_4.562093, whole genome shotgun sequence.
ACCESSION   DAOQDO010000156 DAOQDO010000000
VERSION     DAOQDO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12273_length_4661_cov_4.562093"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKZ53_03590"
     CDS             <1..746
                     /locus_tag="PKZ53_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNJ39548.1"
                     /translation="LLIGRDETLKTVRWLKQLDVPDFEKDYEFVALRHPDEYPFNEGR
                     LVSTAGLDIAIQDFHTHFVEEHVAHSNALHCRLRERGAYCVGPQARFSLNFDRLSPLA
                     QEAAREVGLPVPCLNPFLSIVIRGVELLYAYDEAIRIIDNYDLPDHPSVEVHPVAGTG
                     HGCTEAPRGILHHRYSVDEEGLITDARIIPPTSQNQKSIEQDLQVYISRNLTMPTKEL
                     TWQCEQAIRNYDPCISCATHFLKLEIERS"
     gene            940..1377
                     /locus_tag="PKZ53_03595"
     CDS             940..1377
                     /locus_tag="PKZ53_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNJ39549.1"
                     /translation="MNFIIGIGNDFRSDDAVGLVIARRLKACHINQFTVIEFTSDGAA
                     LLDYFQQAEVVILVDAVSSGATPGTIHRLDAHREVISKQYFQCSTHTVSVAEAIELGR
                     VLGILPQQLLVFGIEAANFSPGVGLSPLVEKVVDQVVEQIQRL"
     gene            complement(1414..1701)
                     /locus_tag="PKZ53_03600"
     CDS             complement(1414..1701)
                     /locus_tag="PKZ53_03600"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNJ39550.1"
                     /translation="MSRHRLTEEQVLLSLVNSPLWERQNNEIRRQFIFPSFREAMVFV
                     NQVAELAEAADHHPDIIINWNKVTLSLTTHDRGGLTALDFDLAHQIDVIQP"
     gene            complement(1625..2797)
                     /locus_tag="PKZ53_03605"
     CDS             complement(1625..2797)
                     /locus_tag="PKZ53_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNJ39551.1"
                     /translation="MDGTALVLCEGAFGTAAGKTAHGLIRFTERYQILGILDSHQAGK
                     DSGEVLDGKPNGIPIFASLPEAVETLGTTPKYLVVGQSSPTGGLLPGGRNIIRQAINL
                     GMNIDSGLRQFLTEDADLPGLAQLRGVSIRDVRKPKDPRQLHLYSGKISQVDCVKIAV
                     LGTDSLVGKRTTAINLRQGLRHVGLKTFLVGTGQTSWLQGMKYCVITDTMMSHYLTGE
                     IEDTVYQAYVNEQPDVILLEGQGSLTDPLRPGGLELLCAARPDAIIVQHAPKRKFHTQ
                     ALNQALIPLKDEIKVIELLTQKPVIAITLNHEGMTDDEIQSLIEEYEIEYRVPTLDVL
                     KEGPARLVSTVLHRFPELVETIHKKRGEAQDDESSPINRRTSPFITGKFPAMGTAE"
     gene            complement(3062..>4661)
                     /locus_tag="PKZ53_03610"
     CDS             complement(3062..>4661)
                     /locus_tag="PKZ53_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39552.1"
                     /translation="LPQVSYRTTEDIRDPMDILQSNALLQASLAESEKRYENLFENAN
                     ELILVVEPVTGRIVDANRHTFEQLLYNRDELLARTVFDLYPAEDHPAIKKRLNPLPAH
                     EHYADSMDAVVLCKDRTHRSVAIRVYDATLMGKPVLVFLMRDITERQQALEGLRQTNR
                     LKDEFLQNISHELRTPLCAIVGWSDLIASGVLTSEQQTTGNEQIRSSSEQLLQLINDL
                     LDVAHMERGIMNLDIELAEISEPVQKAFEIFEFAAKNKGIRLTLNLELNLPLLQQDVA
                     RIQQVVTNLLSNAIKFTPDGGQVTVSVQSLGREIEISVADTGVGISPAALPYIFEGFR
                     QGDGSATRRFGGTGVGLALVKSLIELHGGSVSVESEVHHGSTFRVRLPVPPQPVSGRR
                     RISGNFPALDERSSAPPDEAQVLIIDHDRALLDLAGAMVRSAGYKVILATTLESAKVK
                     LKDAWPTLVLIEVSSVTEGELLLSSLHSILVLQSAKIIAMTTSTNLLDKEAFLKQGFA
                     EYLSKPFKKDQLIAVVRHFFIAEP"
BASE COUNT         1119 a         1186 c         1143 g         1213 t
ORIGIN      
        1 aactgctgat tggacgtgat gaaacactca aaactgttcg gtggctaaaa caacttgatg
       61 ttccggattt tgaaaaggac tatgaatttg tggcacttcg ccacccagac gaatacccgt
      121 ttaatgaagg tcgtttggtg tcaaccgccg ggcttgatat cgccatccag gactttcata
      181 cccattttgt tgaagaacac gtggcccatt caaacgcgct ccactgccgg ttgcgtgagc
      241 gcggcgccta ttgtgtcggt ccgcaagccc ggttcagcct caattttgat cgtctgtcgc
      301 cgctggctca agaagctgct cgcgaagtgg gtctccccgt cccctgcctg aatccgtttt
      361 tgagcatcgt gatccgtggc gttgagcttc tgtatgccta tgacgaagcc atccgcatca
      421 ttgacaacta tgacctgcca gatcacccat cggtcgaagt tcacccggtg gccggtaccg
      481 gacatggttg caccgaagca ccccgcggca tcctccatca ccgatattca gttgatgaag
      541 aaggacttat cactgatgcc cgaattatcc cgccgacttc gcagaaccaa aaatccatcg
      601 aacaggattt gcaggtgtat atctcccgca acctgaccat gccaaccaaa gaactcacct
      661 ggcaatgcga gcaggcaatc cgcaactatg acccgtgcat ttcctgcgcc acgcactttc
      721 tcaagctgga aatagagcgg agttgaatga agaatgaaga atgaagaata tctggttctt
      781 ggttcttggc tcttggttct tggttcttgg ttcttggttc ttggttcttg gaaagtgttg
      841 gtttcaaagt actaagtacc aggcaccagg cactttcttc attcttcatt cttcattctt
      901 cattcttcat tcttcattct tcattcttca ttcctcacta tgaacttcat cattggcatc
      961 gggaatgatt ttcgcagtga cgacgcggtc ggattggtca ttgcccgcag actgaaagcc
     1021 tgtcatatca atcagttcac cgtcattgaa tttacttcgg acggtgccgc cttgcttgac
     1081 tatttccagc aagctgaggt tgtgattctg gtggatgcgg tttcctccgg ggcgactccc
     1141 ggtaccattc atcgtttgga tgcccatcgc gaagtgattt cgaaacagta ttttcaatgt
     1201 tccactcaca ccgtgagcgt cgccgaagcc attgaactgg gccgggttct gggaatactc
     1261 ccgcaacaat tgctggtctt tgggattgag gccgccaatt tcagccctgg ggttgggctc
     1321 tctccactcg tggaaaaagt ggtggaccag gtcgtggaac agattcaacg actataaaga
     1381 ataaaagggc accgctggcg gtgccctttt tgattatggt tgaatgacgt caatctggtg
     1441 agccagatca aaatccaggg ctgtcagacc accccggtcg tgggtggtca acgacagagt
     1501 gactttgttc caattgatga tgatatcagg atgatggtct gcggcttcgg ccagttccgc
     1561 cacctgattg acaaaaacca tggcttcgcg aaagctggga aagatgaatt ggcgccggat
     1621 ttcgttattc tgccgttccc atagcgggga atttaccagt gataaaagga cttgttcttc
     1681 ggttaatcgg tgacgactca tcgtcttgtg cctcccctcg ctttttgtga attgtttcaa
     1741 ccagctctgg aaaccgatgc aaaaccgtgc tgaccagtcg ggcgggacct tctttgagca
     1801 catccagagt tggcacccgg tactcaattt catattcctc aatcaaactc tgaatttcat
     1861 catctgtcat tccttcgtga tttaaagtga tggcaatgac gggtttttgg gtgagcagtt
     1921 cgatcacctt gatctcatct ttgagcggaa tcagagcttg attgagcgcc tgggtgtgaa
     1981 attttcgttt cggggcgtgc tgcacaatga tggcatctgg acgggcggcg cacagcagtt
     2041 ccagccctcc gggtcggagc ggatcagtaa ggctcccctg cccttcgagc aaaatcacat
     2101 ccggttgttc gttcacatag gcttgataga ccgtatcttc aatttcacct gtcagatagt
     2161 ggctcatcat ggtgtcggta atgacacaat atttcattcc ctgcagccac gatgtttgcc
     2221 ctgtaccaac cagaaatgtt ttgagcccga cgtgacgcag cccctggcga aggttgatgg
     2281 cggtggtacg tttccccacg agtgaatctg tcccgagcac cgcaatcttg acacaatcaa
     2341 cctggctgat cttgcctgag tagaggtgta actggcgtgg atcttttggc ttccggacat
     2401 cgcgaatact gacacctcgg agctgggcta acccaggtaa atcagcgtct tcggtcaaaa
     2461 actgacgcaa cccgctgtca atattcatgc caaggttgat cgcctggcga ataatattgc
     2521 ggccaccggg caaaagccct ccggtcggtg aagattgccc gaccaccaga tatttgggcg
     2581 tggtgcctaa agtttcaaca gcctctggta acgaagcaaa aatcggaatg ccatttggct
     2641 ttccatccag gacttcacct gaatcttttc cggcctgatg actgtcgaga atccccagaa
     2701 tctgatagcg ctcagtaaat cgaatcaacc cgtgcgctgt tttcccagcc gcggttccaa
     2761 aagcaccttc acataaaaca agtgcagttc catccataca agttcctttc cttcgggtaa
     2821 taacacgtca agtctggaga gtaccgggcc tgcaggctca cgggtactga aactcagact
     2881 tgctgaaagc tcaccccaaa gttctcattc ttttttggtt tgcaaggcac agtgcatcta
     2941 tgccagggaa ttcaaacctg ttagcgtacc tgactgacat tacatttcta tgacatcgac
     3001 gggggcaatc ggcggcaggt ttttcgctga tactgtgtgg gggcatagaa aaagccacag
     3061 cttatggctc tgctatgaaa aagtgtcgaa caacggcaat caactggtct tttttgaatg
     3121 gtttcgacaa atattcagca aatccttgtt tgagaaaagc ttctttgtca agcagattgg
     3181 ttgaagtggt catggcaata attttcgcgg actgtaaaac caggattgag tgtaacgacg
     3241 agagcaacaa ctcgccttct gttacggagg aaacttcaat caacaccagg gttggccagg
     3301 catctttaag tttaactttg gcagattcga gggttgtcgc caaaataacc ttgtacccgg
     3361 cgctgcgaac catagcacca gccagatcga gtaaagcccg gtcatgatca ataatcaata
     3421 cctgggcctc atctggcggc gctgaagatc gctcatccaa agcggggaaa tttccagaaa
     3481 ttcgtcttcg cccagaaacg ggttggggtg gaaccggcaa ccgaacccgg aaagtgcttc
     3541 catggtggac ttcgctttca actgaaaccg aaccaccgtg aagctcaatc agcgatttga
     3601 ccagcgccag cccaaccccg gttccaccaa aacgacgggt tgccgatcca tctccctgac
     3661 gaaatccttc aaaaatataa ggtaaggcgg caggtgaaat accgacgcca gtgtcagcga
     3721 cgctgatttc aatttcgcgc ccaagcgatt gaactgagac cgttacctga ccgccatcag
     3781 gggtaaattt gatggcattt gagagcagat tggtcacaac ctgttgaatc cgcgccacgt
     3841 cctgctggag caatgggaga ttcagctcta aattgagggt caacctgata cctttgtttt
     3901 tggcagcaaa ttcaaaaatt tcaaaagctt tttgaaccgg ctcgctgatt tccgccagtt
     3961 caatgtccag attcataatg ccccgttcca tatgggcgac atccagcaaa tcattgatca
     4021 attgcagcaa ttgctcggaa gacgaccgaa tctgttcatt tccggtggtt tgttgctcgg
     4081 aggtcaacac ccccgaagca attaaatcgc tccagccaac aatggcgcag agtggtgttc
     4141 ggagttcgtg tgagatgttt tggagaaatt catctttgag gcgattggtt tgccgcaatc
     4201 cttcgagtgc ctgctggcgc tcagtgatat cccgcatcaa aaagaccaga actggctttc
     4261 ccattagcgt ggcatcatag acccgaatgg caactgaacg atgggttcgg tctttgcata
     4321 acacgacggc atccattgag tcagcatagt gttcgtgggc tggaagcggg tttaaccgct
     4381 ttttgatggc cggatgatct tctgccggat ataaatcaaa gactgttcgg gccagaagtt
     4441 catcacgatt gtacaaaagt tgctcaaagg tatggcgatt ggcatccaca atccggcccg
     4501 ttactggctc aaccaccaga atcaattcgt tggcattttc aaacaggttt tcatagcgct
     4561 tttccgactc ggccagcgat gcctgcagca gtgcattgct ctgcaggatg tccattgggt
     4621 ctcgaatatc ttcagttgtt cgataagaga cctgcggaag c
//
LOCUS       DAOQDO010000157         4649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12326_length_4649_cov_3.131694, whole genome shotgun sequence.
ACCESSION   DAOQDO010000157 DAOQDO010000000
VERSION     DAOQDO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12326_length_4649_cov_3.131694"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKZ53_03615"
     CDS             <1..699
                     /locus_tag="PKZ53_03615"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39553.1"
                     /translation="SIKALLFANLEQEARELSQDFIGKLLPLIPDDLNDLSGNPFEQT
                     QILAQLANLYRSHYRNQEAELFYQKALSIAESQLKPEDPITTNILNDLASLFISEARY
                     EEATFLYQQALDIYKHQFGPDHPTIGTSLNNLAVVFHHQRRFNEAQLQYQQALIICEH
                     YFGPHSIETTIILSNLAILFTAQGMFHQAEPLFARAYSPLTSKLGKDHPYTKLVKRNY
                     LSLLRQMGRTEELD"
     gene            950..1420
                     /locus_tag="PKZ53_03620"
     CDS             950..1420
                     /locus_tag="PKZ53_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39554.1"
                     /translation="MKRLDQVIDVIIERFDAENNLMVKEHTITDMIAQNGVSILTRNQ
                     IPVGDFLLFKTADGYFQSSAVVKSARFAEDFIQRLTLEFVGEDWQKKWIYAHSETVSP
                     LLEEFYDIAEEMSLLLQILMQDREQGQAPDEVFLLQLQENVDRLRSITFRIRKA"
     gene            complement(1395..4640)
                     /locus_tag="PKZ53_03625"
     CDS             complement(1395..4640)
                     /locus_tag="PKZ53_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ39555.1"
                     /translation="MLTTDKPVYQPGQVLHLRAIALEQTSRLALAGVPVEFEVNDSTY
                     SEVFRTVSLTSKSGIATVDWTIPETQPTETLTIQAQVMDSTSEVIGSTLQQVKISRYE
                     LPQFVVTAQTDRSFYLPGQTATVNAQALYLSGEPIREGTVRVVRRRDVWNQEKQTTTQ
                     VEEIITEVKANLSGQVQFPIDLTRDQDNFSGGEYDWFRDVSFDVEWNDPASHKTESYR
                     LYLRLSKTPIHLYVLSARKPLDPQVPGTVYVATCAADGTPCAAEFELTSDWLVTFGPN
                     RTNEERQTVSLGKFKTSRYGLARVELPVKELAKPDVRFSTCLTGLDRDEFEIGFTPES
                     SGRGPFDLKTQKTMYRPGEPIDVTLWSTLPECWGQVLVLRGNEIVVSQAVLLKPGKTS
                     VTLPYQPGFKGNVQVTVTSPHFSSEWILPSQWIVYVPDEFPRLTIDPLKKTIKPGDEA
                     EISCQIQTTDGKPVHGVIGVSVVDCAIPRQIQSDEEGTIPICYDGGLNQAAARWMIGR
                     LSLSDLSRLDPGQPIPDDLQLVAEVLAWQWYSEREFFREDTDPWGAQFIAHFFQPGIQ
                     KQLEPLLNLVKTQVAQATTRPETVEQVEQLVAAGGQSLTALRDPWGMPYQVTVMRRQA
                     RDVLEIHSCGPDRTPNTIDDFLAGECGWSYSAALHTAIQQASTRYFQHTGNYIRDYDT
                     LKTEIARQGIDLDHLRDQWGRGLFFDFPILKSCYAILVRSAGERGQASPSDEFYDGSE
                     LDRGGFLLTASVLDFFHEAREQFKATVFHSGEAILKAENREAALQAALKQNGLDLEQL
                     RDPWGSPYFVSFRKKSLGELPESKTSEWWIISLCSPGPDKRHDGSGFTLAASSVLLKV
                     PFSGDTPNLQAKPGSSRPASGAMAGLVSDEFGNALSQVRLEMKNETTGRLTYHWTDQD
                     GTYLINDLPPGRYEITLLHSSNIVYIYDTVGVTSGKLNLVDAVIGVREITEAEIGRMQ
                     SKPTFMNMWYAPNYFHTTPMLANRKHVVPVSTQNPPTLPKLRQDFTETALWAPLLATN
                     HQGRVRVKLKFPNVVTTWRLSVVALTADGQIIEKEESLPVGKAIMPCECEK"
BASE COUNT         1178 a         1208 c         1059 g         1204 t
ORIGIN      
        1 tcaatcaaag ctttgctttt tgctaaccta gaacaggaag caagagaact gagtcaggat
       61 tttatcggaa agctattgcc acttatccca gatgacctga acgatctctc agggaatccg
      121 tttgagcaga cacaaatctt agctcaatta gctaatctct acagatccca ttatagaaat
      181 caggaagctg aattgtttta tcaaaaggca ctttcaattg ctgaaagtca attaaaacct
      241 gaagatccga ttacaaccaa tattctcaat gacttggcga gtttatttat ctctgaggca
      301 cgctatgaag aagccacgtt cttatatcaa caagccctgg atatttataa gcatcaattt
      361 ggaccggacc atccaactat cggtaccagc cttaataacc ttgccgtggt atttcaccat
      421 caacgaagat tcaacgaagc ccaattacaa taccagcaag cgctgataat ttgtgaacat
      481 tattttggcc ctcattcaat agaaacaacc ataattctca gtaacctggc tatcttgttt
      541 acagctcaag ggatgtttca tcaagctgag ccattgtttg ctcgtgctta ttcacccctc
      601 acatccaaat taggaaaaga tcatccatac accaaattgg taaagcgaaa ctacctctcc
      661 cttcttcgcc aaatgggtcg caccgaagaa ctcgattgat tcccttcgtc atcccagttc
      721 aattttttgc aggctcaagc ttgaattctg gactttacaa ccaaactaga attttctttc
      781 agaaaactgg aaatttcctt ccaacgaatg gtaagatgtt gccctgactg agcgttcttc
      841 gtggctcacg ttccttgccc tgaaaaacct cctgccacaa acttcacaac gcttccttct
      901 atcgccgcct tactttccac tcggctgcag agtggctttg gagaattcca tgaaacgtct
      961 cgaccaggtc attgacgtca ttatcgaacg gtttgacgct gaaaataacc tcatggtcaa
     1021 ggaacacacc atcaccgata tgattgcgca aaatggcgtt tcgatcttga cccgcaatca
     1081 gataccggtt ggggatttcc tgctgtttaa gacagcggat ggctattttc aatcatcggc
     1141 ggtcgtcaaa tctgcccggt ttgccgagga ttttatccag cgcctgacgc tcgaatttgt
     1201 tggtgaagac tggcaaaaga aatggattta tgcccattcg gaaacggtga gcccgctcct
     1261 tgaagagttt tatgacatcg ctgaagaaat gtcgttgctg ctccagattc tgatgcagga
     1321 ccgcgaacag ggtcaggccc ccgacgaagt gtttctcctc cagctccagg aaaacgtaga
     1381 ccgcctgcgc agcattactt ttcgcattcg caaggcatga tggcttttcc gactggcaaa
     1441 gactcctctt tttcaataat ttgaccatcc gctgtcaaag ccacaactga aagcctccag
     1501 gtggtaacaa catttggaaa ctttaatttg acacgcaccc ttccttgatg atttgttgcc
     1561 agaagtggcg cccagagcgc ggtttcggtg aaatcctgtc tcagttttgg cagagtgggt
     1621 ggattctgtg tgctgacagg cacaacgtgt ttcctgttgg ccagcatggg cgttgtatga
     1681 aaataatttg gcgcatacca catgttcata aaggtaggct tgctttgcat acggccaatt
     1741 tcagcttcgg tgatttccct gacaccaatc acggcgtcca ccagattgag cttcccagag
     1801 gtaaccccaa ctgtatcata tatatataca atgtttgatg agtggaggag agtaatttcg
     1861 tatcgtccag gcggcaaatc attgatcagg tacgttccat cctggtcagt ccagtggtag
     1921 gttaatcttc ccgtagtttc gttcttcatt tccagccgaa cctgactgag cgcgtttcca
     1981 aattcatcac tcaccaaacc agccatcgca ccagaagctg gcctggatga gcctggtttt
     2041 gcctggaggt tgggcgtatc tccagaaaat ggaaccttca gcaaaaccga tgaggccgcc
     2101 agtgtaaacc ccgagccatc gtgccttttg tcgggcccag ggctgcacag tgaaatgatc
     2161 caccattctg atgttttgga ttcgggcagt tcacccaacg attttttcct gaagctcaca
     2221 aaatagggac tgccccacgg atcccgaagc tgttccaaat caaggccgtt ttgtttgagg
     2281 gcggcctgca acgcagcttc gcggttttca gctttgagaa tcgcttcccc tgaatgaaac
     2341 acggtggcct tgaattgttc ccgtgcctcg tggaagaaat ccaaaacgga tgccgtcaga
     2401 agaaatcctc cccggtccaa ttcgctgcca tcataaaatt cgtcgctcgg agaggcttgc
     2461 ccacgctcac ccgcacttcg caccagaatc gcataacagc ttttcaaaat cggaaagtca
     2521 aaaaacaatc cgcgccccca ctgatcgcgc aaatgatcga gatcaatgcc ctggcgcgca
     2581 atctcagtct tgagcgtgtc atagtcgcga atgtagttgc ctgtgtgctg aaaataacga
     2641 gtgcttgcct gttgaattgc cgtgtggagg gccgctgaat acgaccatcc acattcgcca
     2701 gccagaaaat catctatggt attgggggtt cgatctggtc cacaactatg gatttccagc
     2761 acatcgcggg cttgccgccg catcaccgtg acctgatagg gcattcccca cggatcacgg
     2821 agtgcggtca agctttgacc accagccgcc acaagttgct caacctgctc aaccgtttca
     2881 ggtcgggtgg ttgcctgagc aacctgcgtt ttcaccagat taaggagtgg ttcaagctgt
     2941 ttctgaatac ctggttgaaa aaagtgagcg ataaattgtg ctccccaggg atcggtgtct
     3001 tcacgaaaaa actctcgctc tgaataccat tgccacgcaa gcacttccgc caccagttga
     3061 agatcgtccg gaattggctg gcctggatca agccgcgaga gatctgatag cgaaagccgg
     3121 ccaatcatcc aacgcgcagc agcttgattg agtccaccgt catagcagat tggtatggtt
     3181 ccctcttcat cgctctgaat ttgcctcgga atcgcacaat caaccactga caccccaatt
     3241 acaccatgaa ctggtttccc atctgtggtt tgaatctggc atgaaatttc tgcttcatcc
     3301 cccggtttta ttgttttttt caaagggtca attgtcagtc gtggaaactc atccggcaca
     3361 tacacaatcc actggctggg tagaatccat tcagaggaaa aatgagggga cgtcacggtc
     3421 acctgtacat tccctttgaa acctggctgg tatggaagcg tcacactcgt ttttcctggt
     3481 ttgagcagca cagcctggct gaccacaatc tcatttccac gcagtaccaa aacctgtccc
     3541 caacattcgg gcaatgttga ccagagcgtg acgtcaatcg gttcaccagg gcgatacatc
     3601 gttttttgtg tcttcaagtc aaatggtcca cgcccagatg attcaggagt aaatccaatc
     3661 tcaaactcgt ctcgatcaag ccccgtcaga caggtgctga atcgcacatc aggtttggcc
     3721 agctctttca caggaagttc aaccctggcc agtccgtaac gactggtttt gaatttccca
     3781 agcgaaactg tctggcgttc ttcattggtt cgatttggcc caaaagtgac aagccagtcg
     3841 gatgtcagtt caaattctgc cgcacagggc gttccatcgg ctgcacaggt ggcaacatac
     3901 accgtaccag gaacttgagg gtccaacggt tttcgagctg aaaggacata caaatgaatc
     3961 ggagttttcg ataatcgcag gtacagccga taggactcgg ttttgtggct ggctgggtcg
     4021 ttccattcca cgtcgaatga aacatcccga aaccagtcat attcaccgcc ggaaaaattg
     4081 tcttgatcgc gagtaaggtc aattggaaac tggacctgtc cggacaggtt ggctttgact
     4141 tcggtgataa tttcctcgac ttgcgttgtg gtttgttttt cctgattcca aacatcacgg
     4201 cgacgaacca cccgcaccgt cccctcacgg atcggttctc ctgaaagata aagcgcctgt
     4261 gcattcacag ttgcggtctg accgggaagg taaaatgaac ggtcagtttg agcggtcacg
     4321 acaaactggg gtaactcata ccggctgatt ttgacctgtt gcaaggttga gccaatcacc
     4381 tcagaggttg aatccatcac ctgcgcctga atggtcaacg tttcggttgg ctgggtttct
     4441 ggaatcgtcc agtcaacagt cgcaattcca gatttcgacg taaggctgac ggttcgaaag
     4501 acttcagaat acgttgaatc gttcacctca aattccactg gtacaccagc cagagccagc
     4561 ctggaagttt gttccagggc aattgcccgc aaatgcaaaa cctgaccggg ctggtagacg
     4621 ggcttatctg tagtgagcat cacctgggc
//
LOCUS       DAOQDO010000158         4648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12334_length_4648_cov_2.732637, whole genome shotgun sequence.
ACCESSION   DAOQDO010000158 DAOQDO010000000
VERSION     DAOQDO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12334_length_4648_cov_2.732637"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKZ53_03630"
     CDS             complement(<1..1149)
                     /locus_tag="PKZ53_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNJ39556.1"
                     /translation="MKKWLAKFLTDDFKTELKSVIISGVITTFLLGGAILSLVVAILA
                     TQVGEHRTAEVLYILSLVLVMAGGVYAIPRLMKRIRWNFLQFNISYTATPGTAFFMAI
                     LVVVGLAAFNTGNNLLYLIFSVLLGLILTSGVVSETGLRELDIGLRFPDHIFAGQDVL
                     LEISVTNHKFLIPSFSLSVGVELSNQPVPTGMEKVRQWLLGSTLAGELSKLTHFIVVP
                     SHQRLRQTIQHSFSRRGRYEIKGFTVSTQFPFGFIQKTRRIEAQGELIVYPELEKRRD
                     LLSGLSQLMGTQEHFFKGTGVDLYAIRQYQSGDNLRHIDWKATAKVRRTMVKEFARED
                     ERRISIYFDNRAPKEITPEFLNAFEAAVKRAASIAAQLVESNIQVRLLT"
     gene            1301..2176
                     /gene="murI"
                     /locus_tag="PKZ53_03635"
     CDS             1301..2176
                     /gene="murI"
                     /locus_tag="PKZ53_03635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNJ39557.1"
                     /translation="MTTSTLTQPNQPIGIFDSGVGGLTVYRAIREQLPFEDIVYLGDT
                     ARIPYGTRSLVTVRRYALEDAAFLVSCGIKLLVVACNTASSTAMDVLTDSLSIPVIGM
                     IGPGAQRAVNLTRNGRIGVIGTEATVGSGAYTRAIHQLLPEAVVIERSCPLFVPLTEE
                     GWASHQVTALVAEEYLKPFARDEIDTLVLGCTHYPILRTLIQQVVGENVHLVDSGASA
                     AEEVLTLLSGQELASPNPRAQQTRFCVTDTGQRFRRIAEIFLGHPMDQPESVDLWALN
                     NDKVKLQTDIPHRDE"
     gene            2230..2952
                     /gene="rph"
                     /locus_tag="PKZ53_03640"
     CDS             2230..2952
                     /gene="rph"
                     /locus_tag="PKZ53_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNJ39558.1"
                     /translation="MRPDGRANNALRRVSITPGISKHAEGSALIEFGDTRVMCTASVE
                     ERVPPFLKGKGLGWITAEYSMLPRATDVRTPREVGRGGPSGRTHEIQRLIGRSLRAVA
                     IMEKLGERTIYLDCDVLQADGGTRTTSITGAFVAFVLALRKLQKDNKLKDLPISDYVA
                     AVSVGIVGGTPVLDLNYIEDSQADVDMNIVCTGDGRFVEVQGTAEHKPFTPEQMNSMI
                     TLARQGIDELVGHQRKILGILD"
     gene            3057..3656
                     /locus_tag="PKZ53_03645"
     CDS             3057..3656
                     /locus_tag="PKZ53_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39559.1"
                     /translation="MNVVVRLILKGIFGCIGLALIGINLPVLAQKIEVPSTSETPPAI
                     GKRVFADVEGGYEIELDNGWRTELAVENNGRRLTEIIFRDRSYALLKVKKEKAPDEPL
                     VEGDLVKTLITREMENDLRFRPDYKFVSQERFVNSVARGALLQFTYKRVGKLMTGRYY
                     YLQTTPDTVWVLRFIGEKTYLDPFRYQTDQLARSFKPLP"
     gene            3776..4582
                     /locus_tag="PKZ53_03650"
     CDS             3776..4582
                     /locus_tag="PKZ53_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39560.1"
                     /translation="MQLQEKDFVDLFKALLRRSIWLIILAALGAGAGYFWTQRQPTIY
                     QAMAFIELGRIDQEPIEEPHLLAEIVNAPGFLAAQGTETNTESDPAPAEGKKKKKKQA
                     GGAGRLTAECLETGRKRERIVYLIRLTARSEDKAAARATVEAGATAVIDRTNKKFTDA
                     VKLYREREIELVTRLEGLRKSPAGNISDVLLCERELFDVRSQLLPQRTFATQLARPIT
                     APEVISSPQPARNTVLAAGSAFLFGALLVIGLEFIRPAPMKNEEKRQNEE"
BASE COUNT         1194 a         1127 c         1216 g         1111 t
ORIGIN      
        1 ggtcaagagt cggacctgaa tatttgattc aaccagttga gcggcaatcg aagcggctcg
       61 tttgacagca gcttcaaagg cattgagaaa ttcgggagtg atttcctttg gtgcccgatt
      121 atcaaaatag atcgagatgc gtcgttcatc ttcacgggca aattctttca ccatcgttcg
      181 ccgaaccttg gcggttgctt tccaatcaat atggcgcagg ttatcgccgc tttgatactg
      241 gcgaatggca tataaatcta cccccgtgcc tttgaaaaaa tgctcctgtg ttcccataag
      301 ctggctgagt ccactcagta aatcacgccg cttttcaagt tctggataca caatcaattc
      361 accctgggct tcaattcgac gggttttctg gataaacccg aacggaaact gggtgctgac
      421 ggtaaatcct ttgatttcat agcgtccacg gcgggagaat gaatgctgga tggtttgccg
      481 aagccgttgg tgacttggga ccacaataaa atgggttagc ttcgaaagct ctccggccag
      541 ggttgagccc aggagccact gacggacttt ctccattccc gtgggtaccg gctgatttga
      601 gagttcaacc ccaactgaga gcgaaaacga tgggatcaga aacttgtgat tggtgactga
      661 aatttcaagc aggacgtcct gtccggcaaa aatgtggtct ggaaaccgaa gtccaatatc
      721 gagttcccga agtccggttt cagaaacaac ccctgacgtc aaaattaacc ccaacagaac
      781 cgaaaagatc agatagagca gattgttccc ggtattgaag gcggccaggc cgaccactac
      841 cagaatggcc ataaaaaaag cggttccggg tgtggcggta taactgatgt taaactggag
      901 gaaattccag cgaatccgct tcatgagccg ggggatggca tacacaccac cagccatgac
      961 aagcaccagg gaaaggatat agagtacctc ggcggttcga tgttcaccca cctgggttgc
     1021 caaaatggcg actaccagtg atagaatcgc gccccctaac aaaaatgtcg tgataacgcc
     1081 actgataatc acgcttttga gttcggtttt gaaatcgtca gtcaggaatt tcgccagcca
     1141 ttttttcacg aaagcacctc gatttgccga gtccggcctg aaattggatt gcatcataaa
     1201 ccaaaatcgg gtgagagcaa tctacttggt gcctggtgac tggtttcgaa aaccctgacc
     1261 cttgacccct gacccctgaa tccgacaggt atccgtcaac gtgaccacat ctactctgac
     1321 ccaaccaaat cagcccatcg gaatctttga ttccggtgtg ggtggtctca ctgtttatcg
     1381 agccatccgt gagcaactcc ctttcgaaga tattgtgtat ttgggcgata cggcgcgcat
     1441 tccgtatggc acccgctcac tggtgactgt gcgccgctat gcccttgaag atgctgcttt
     1501 tctggtttcc tgtggcatca agctgctggt tgtggcctgc aacacagctt catcaaccgc
     1561 catggatgtg ttgactgact cgctctcaat tcccgtgatt ggaatgattg gaccaggtgc
     1621 ccagcgggcg gtcaatctga cccgaaatgg tcggattggt gtcattggaa ccgaagcgac
     1681 cgtcgggagc ggtgcctata cccgagctat tcaccagtta ctccccgaag cggttgtgat
     1741 cgagcgcagt tgcccgttgt ttgtcccatt aactgaagaa gggtgggcca gccaccaggt
     1801 tacggctctg gtcgccgaag agtatttaaa accctttgcc cgtgatgaaa ttgacacact
     1861 ggtattgggc tgtacccatt acccaatctt acggacctta attcaacagg tggtcggtga
     1921 aaatgtccat ctggtggatt caggtgccag tgctgccgaa gaagttctta cgctactctc
     1981 aggtcaggaa ctggcgagcc ccaacccccg tgcccagcaa acccgatttt gtgtcaccga
     2041 caccggacag cgattccggc gaattgccga gatttttctg ggccacccaa tggatcagcc
     2101 tgaatcggtt gacttgtggg cactgaacaa cgacaaagtg aaattacaga ctgacatccc
     2161 gcatcgagat gaatagctga gtgttgatat tgaaaatttt gaatcccagg tagatgggaa
     2221 ggagacaaca tgcgtccaga tggaagagca aataatgcat tacgccgcgt atcaattacg
     2281 ccaggcatca gtaagcacgc cgagggttct gctttaatcg agtttggcga taccagagtc
     2341 atgtgcaccg caagcgttga agaacgtgtc cctccatttt taaaaggtaa ggggctgggg
     2401 tggattacgg ctgaatattc aatgctcccc cgagccacag acgttcgcac cccacgtgaa
     2461 gttggtcgag gtggtccatc ggggcgaacg cacgaaatcc agcggttgat tggccggagt
     2521 ctgagagctg tcgccattat ggaaaaattg ggtgaacgga caatctacct tgactgcgat
     2581 gtgctgcagg ccgacggtgg cacccggacc acctcaatca ctggtgcctt tgtggcattt
     2641 gtccttgcgc tgagaaaact ccagaaagac aacaaactca aagatttacc catctcagac
     2701 tatgttgctg ccgtcagtgt tggcattgtc ggtggcacgc cggtgctgga cctcaactat
     2761 attgaagatt cacaggccga tgtggacatg aacattgtct gtactggcga tggtcggttt
     2821 gtggaagtcc aaggaacggc tgaacataaa ccgtttaccc cagagcagat gaattcaatg
     2881 attaccctgg cccggcaggg aattgacgaa ctggttggcc atcagcggaa aattctgggg
     2941 atattggatt gatccctggt gcttggtgcc tgggaatcaa tatcttccaa ggatcaagaa
     3001 cgaaaacgac tcaactcgtt atgaactgag tgtctcgtaa accctggaaa tactgtatga
     3061 atgtggttgt tcgattgatt ttgaaaggta tcttcggttg tattggactg gctctgatcg
     3121 gaataaacct gccggttctg gctcagaaaa ttgaagttcc atccacttca gagacgccgc
     3181 cagccattgg taaacgcgtt tttgccgatg tcgaaggtgg gtatgaaatc gagcttgata
     3241 atgggtggcg caccgaactg gcagtcgaaa acaatggccg tcggcttacg gagattattt
     3301 tccgcgaccg gagttacgca ttgttgaaag tcaagaaaga gaaagccccg gatgaacctc
     3361 tcgtcgaggg ggatctggtg aaaaccctga ttactcgtga gatggaaaac gaccttcggt
     3421 ttcgacccga ttacaaattt gtgagccagg aacgatttgt caattcggtg gcgcgtgggg
     3481 cattgctcca gtttacttat aaacgagtcg ggaaattaat gacgggtcgt tactactacc
     3541 ttcagaccac acctgacaca gtgtgggttt tacgttttat cggagaaaaa acctacctgg
     3601 acccattccg atatcaaacc gaccagttag cacgcagttt taaaccattg ccctaagggc
     3661 ctgtctggcg ttactaccag ttttttcggg tcaacgatgt cggggccgca gccgcctgga
     3721 gttaactcca agccaggtga ctttggacct gacccggttg ccttcccgca catgtatgca
     3781 attgcaagaa aaagactttg ttgatttatt caaagcgctg ctgaggcgct cgatctggct
     3841 gattatcctg gctgcccttg gagccggtgc cgggtatttt tggactcagc gccagccgac
     3901 aatctatcag gccatggcgt ttatcgaact tggtcggatc gaccaggaac caatcgaaga
     3961 accgcacctg cttgctgaaa ttgtcaatgc ccccggtttt ttggctgctc aggggactga
     4021 aaccaatact gaatcagatc cggcccctgc agaaggaaaa aagaagaaga aaaaacaggc
     4081 tggcggtgcc gggcgactga cggcggaatg ccttgaaaca ggccgtaagc gcgagcgaat
     4141 cgtctatttg atccgactca ctgctcgaag tgaagacaaa gcggctgcca gagcaacggt
     4201 ggaagctggc gccacagcag tgattgatcg gacaaataaa aagttcactg atgccgtcaa
     4261 gctgtatcga gagcgtgaga tcgaactggt tactcgcctg gaaggacttc ggaagtcacc
     4321 cgccggaaat atctcagatg tcttgttgtg tgagcgggaa ctctttgatg tccgctcaca
     4381 gttattgccg caacgcactt ttgccacgca acttgcacgc ccaattacag ctcctgaggt
     4441 tatttcttca ccacaacctg ctcgaaacac agtgcttgcc gccgggagcg cttttctatt
     4501 cggggccttg ctcgttattg gtttggagtt tatccgcccg gctccaatga agaatgaaga
     4561 aaaaagacag aatgaagaat gaagaaaaaa gacagaatga agaatgaaga atgaagaatt
     4621 gagaatgaag aatgaagaat gaagaatt
//
LOCUS       DAOQDO010000159         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12339_length_4646_cov_3.207362, whole genome shotgun sequence.
ACCESSION   DAOQDO010000159 DAOQDO010000000
VERSION     DAOQDO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12339_length_4646_cov_3.207362"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PKZ53_03655"
     CDS             <1..1486
                     /locus_tag="PKZ53_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39561.1"
                     /translation="SPYEPLRSFRVDKNNFAPRIGLAWAPDFKDGIGHILFGNSGEGS
                     VRAGYGVSFDVAYGNLGTLQLPPQFQQELDAQAGDGGAFGTATNFIANGGLGNTPIPP
                     ITPADARAATSSFIADRTLPYSMTWSLSYQRQLHRDWSVELRYLGTRNVKQIAQVRLN
                     GGNVPFAAAGFSLPTYLNASQVPDRNTLDNMPTLDDLFARSFYGTGIGGLGIADFQQP
                     TVTAFLPIGSSTYHAGSVDLTKRFSYGYTASAAYTYSKTIDLGTNDLFTSFINPRRAQ
                     DGFDISKDRGPSALDRPHRFVASVIWELPFFRKNANRLVRSLLGGFQANVIYTAESGQ
                     PFSALSATDSNLNVDTAGDRTVFNANGVPGTGSGVTAVTNSRGQIVGYLARNGNAQYI
                     QAGRGVIATAGAGTLRAPGINNFDISVFKDFEIREGWRMQFRCEMFNAFNHEQLTVGG
                     GSVFQLLTNATNLSYANAASPTFNDPRIFAGRPRVIQLGLKLIF"
     gene            1910..3856
                     /locus_tag="PKZ53_03660"
     CDS             1910..3856
                     /locus_tag="PKZ53_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNJ39562.1"
                     /translation="MKWRVWGWIFFVVVAFGVMAPISRGFTQETAKDRMRVLEAQLWE
                     IQLKVLQGEILGLQKQSPLSEKKQKKLTEQLAQQQQMQQAYDKATQELKVARVAEDLD
                     IVNVTGLHERFNQIKTQFLEAQIQVLQEEIELREQASQVKQDDRQLRMLHLKVKSLVQ
                     EQHRLTKGFTANQGGPMLFCSSLSSALPPSGSGGSDCTPGIEQFNTSVNVPTNFTVGD
                     VNVRLFLSHEYPSDLKISLQSPLGTSAVIVEDTGGSAFGSFGTTCSPLPNFVIDDEAS
                     SFVPDVTGTYTGSFLPSFEESPGDSLSIFDGEPAQGSWTLSICDDAESDAGTFNCWCL
                     EISAGQPIEQPVLFSADTTNNRIQAFDGFTWNVVAGGAAGSALGQFRLPEAVAFGLGG
                     SPRLYVADTGNNRIQWSTDGGTTWQLFASIGSAQNQVRAPQGLAVDSEGNLYVADTGN
                     GRVLRFDDGVPGFGFVLASNGAGSGQVGAPHGLAIDASFTLFIADQLNSKILKLANAN
                     TIALSNKATLVASLGVGLNKVKNPQGVAVDDSGNLFIADTGNNRVLQFPNGNANQGKA
                     LAQVGAGLGQVNAPEGVTVSRFQNNTLSRVVFNGPYLVVSDTGNNRIQGRLVANTAQQ
                     WDLVGFPNGSGTGTGQFRRPSKIR"
     gene            complement(3980..>4646)
                     /locus_tag="PKZ53_03665"
     CDS             complement(3980..>4646)
                     /locus_tag="PKZ53_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HNJ39563.1"
                     /translation="GIDFVSTPLEDIPAMAAAYLADLKEVQPTGPYQLCGWSMGGLIA
                     YEVAQQLTRQGEAVAQLILLDVEVTTPEPEPVVENAVLLASLVDPLLGVTPDDLRSLD
                     LDQQLAFVLERKQTSPDLQGLLGLEHARRVFQVVKLNSIAARKYECKPYSGNVIVLRS
                     EEHGPDEPLDLGWGRLVQGEVERVQVRGTHQKLLREPFVEKVAQEVRERLRVEEIERK
                     KSG"
BASE COUNT         1080 a         1223 c         1192 g         1151 t
ORIGIN      
        1 ctcgccctac gaaccgttgc gcagcttccg cgtagacaag aacaactttg caccacgtat
       61 tggcctggcc tgggcgccag acttcaaaga tggcatcgga cacatcctct ttgggaacag
      121 cggcgaaggt tcagtccgcg ctggatacgg tgtctcattt gacgttgcct acggaaacct
      181 gggcaccctc cagttgccac cccaattcca gcaggaactc gatgcccagg ctggtgacgg
      241 cggggcattt ggaaccgcta ccaatttcat tgccaatggt ggtttgggca acaccccaat
      301 tcctccgatt accccagccg atgcacgggc agcaacctcc tcatttattg ctgaccgaac
      361 gttaccgtac tcgatgacct ggagcttgag ctatcagcgc cagttgcacc gtgattggag
      421 cgtcgaactg cgttatttgg gtacccgcaa cgtcaagcag attgctcagg tccgtctcaa
      481 tggtggaaat gtgccatttg ccgctgctgg tttcagcctg ccgacctatc tgaatgcgtc
      541 acaggtgcca gatcgcaaca cgctcgacaa catgccaacc cttgatgacc tgtttgcccg
      601 ttccttctat ggaacaggga ttggcggttt ggggattgct gacttccagc agccgaccgt
      661 gacggccttc ttgccaatcg gcagttcaac ctaccacgct ggttcagttg atttgaccaa
      721 gcgcttctcc tatggctaca cggcatcagc cgcctacacc tatagcaaga cgattgacct
      781 cggcaccaac gatctgttta ccagcttcat caacccacgc cgtgcccagg acggatttga
      841 cattagcaaa gatcgtggac cgtcagccct ggatcggcca catcgctttg tggcgtcagt
      901 gatttgggaa ctgccgttct tccgtaaaaa tgccaaccgt ctggtccgca gcctgttggg
      961 tggtttccag gccaacgtga tttacacggc tgaaagtgga cagccattct cagcactgag
     1021 tgcgactgac agcaacctca acgttgacac cgcgggtgac cgcaccgtgt ttaacgccaa
     1081 tggtgttcca ggcaccggca gcggtgtgac cgccgtgacc aactcccgtg gtcaaatcgt
     1141 tggctacctg gcccgcaatg gcaacgccca gtacatccag gctggtcgtg gcgtgattgc
     1201 gacggctggt gcaggaacgc ttcgcgctcc aggcatcaac aactttgaca tttcagtgtt
     1261 caaggatttt gaaatccgcg aaggctggag aatgcagttc cgctgtgaaa tgttcaatgc
     1321 gttcaaccac gaacagttga ccgtcggtgg tggcagcgtg ttccagttgc tgaccaatgc
     1381 gaccaacctg tcctatgcga acgccgcgag cccaacgttt aacgatccgc gaatttttgc
     1441 tggtcgtcca cgtgttatcc agttgggtct gaaattgatt ttctaagtaa gttggaagtt
     1501 tctctaactt cttcctttca ggcagctttt ctttggaaaa gctgcctttt tttatgcagg
     1561 ctggtacaaa gatttttcaa aaaatctctg tttctgattt gacgaattcc atcatttcaa
     1621 gcgagaatga atccagttta accccatttt tttctggtca cctgaagtat tcacttcatc
     1681 aaaaagcacc tctttctttc tgtgtattct gctttcaaaa ctctgttcac tatcgagagt
     1741 gatcaaaact ccttgccatc aacagggtca accaaatgat tgattcttca atgtgttgag
     1801 ccattttcat cagaaattct gccggtaagt ttttccgggc caggtatgga cgatggacat
     1861 ttaaaccggt ttttaccttg taaaaacaac actttccagg agatagccta tgaaatggcg
     1921 tgtatgggga tggatatttt ttgtggtcgt tgcctttggg gtgatggcgc cgatttcacg
     1981 aggttttaca caggaaaccg cgaaggatcg gatgcgggtg ttagaggccc aactttggga
     2041 aattcaactc aaagtccttc agggtgaaat cctcggtttg caaaaacagt cgccgctgag
     2101 tgagaagaag caaaaaaaat tgactgagca acttgcccag caacagcaaa tgcagcaggc
     2161 ctatgacaaa gccacccagg aattgaaggt tgccagggtt gccgaagacc tggacattgt
     2221 gaatgtcact ggattgcacg aacgctttaa ccagatcaaa actcaatttc tggaagctca
     2281 aatccaggtt ttgcaggaag aaatcgagct tcgtgaacag gcaagccagg tcaaacagga
     2341 tgaccggcag ctccgcatgc tccacctcaa ggtgaaatcc ctggttcagg agcaacaccg
     2401 gctgaccaaa ggctttaccg ccaaccaggg cggaccaatg ctcttttgca gttcactgag
     2461 cagtgccctt ccgccatcag ggagtggggg gagtgactgc acgcctggaa tcgaacagtt
     2521 caatacctca gtgaatgtcc cgaccaattt caccgttggt gatgtcaatg tgcgattgtt
     2581 tttgagccat gaatatccat cagatctgaa aatttcactt caatctccac ttgggacgag
     2641 cgcggtcatt gttgaggata cgggtggaag tgcgtttggg tcgtttggca ccacctgctc
     2701 cccgttgcca aactttgtca ttgacgatga agcgtcttcc ttcgtccctg atgtgaccgg
     2761 cacctacacc ggctcgttcc tcccaagttt tgaagagtca ccaggtgatt ccctttctat
     2821 ttttgacggt gaacccgctc aagggtcgtg gacgctctcg atttgtgatg atgcagagag
     2881 cgatgccggg acatttaatt gctggtgtct ggaaatttcg gcagggcaac ctatcgaaca
     2941 gccagtcctc ttttccgctg ataccaccaa taaccggatt caggcatttg acggctttac
     3001 ctggaatgtc gtcgctggcg gtgcggcggg cagcgccctt ggtcaattcc gattgcctga
     3061 ggctgtcgcg tttgggttag gtggaagtcc ccgactgtat gtcgctgaca ccggaaataa
     3121 ccgcattcag tggtcaaccg atggtggtac aacgtggcaa ctgtttgctt cgattggctc
     3181 agcccaaaat caggtccgcg cacctcaagg tctggcggtt gactcggaag gaaacctcta
     3241 tgttgccgat accggaaatg gacgggtgct gcggtttgac gatggtgttc caggatttgg
     3301 gtttgtttta gccagcaatg gcgccggaag cggccaggtt ggtgccccgc acggattggc
     3361 gattgatgcc agcttcacct tgtttattgc tgaccagttg aacagcaaaa tcctgaaact
     3421 ggccaatgcc aataccattg ccctttcaaa taaagccacc ctggtggcgt cactcggagt
     3481 tgggttaaac aaagtcaaaa acccacaagg tgtcgcggtt gacgatagcg gcaacctctt
     3541 tattgccgac acgggcaaca accgggttct gcaatttcca aacggcaatg ccaatcaagg
     3601 gaaagcactg gcccaggttg gggccggttt gggacaagtc aatgctccag aaggtgttac
     3661 tgtcagtcgg tttcaaaata atactttgag ccgcgtggtg ttcaatggtc catatctggt
     3721 ggtcagtgac acgggaaata accgtattca aggccgcctg gtagccaaca cggcgcaaca
     3781 gtgggatctg gttggcttcc cgaacggaag cggaaccggc actggtcagt tccgacgacc
     3841 aagcaagatt cggtagtttt tagggctgaa gaagttgggc tgaagatatt gggctcaggg
     3901 cttggggctg aagactcgca agctcggggc ggaagacatc gggtttaata ccctgagccc
     3961 aacttcttca gcccatactt cagcctgact tcttccgctc aatttcttca actcgaagcc
     4021 tttctcgtac ttcttgagca actttctcaa caaaaggttc gcgtaacaat ttctggtgtg
     4081 ttccgcgaac ctgaactctt tccacctcac cttgaaccag tcttccccag cctaaatcca
     4141 gcggttcatc cggtccgtgt tcttcggatc tgagcacaat cacgtttcca gagtaaggtt
     4201 tacactcata ttttcgtgcg gcgattgagt tgagtttgac cacctgaaac acccgtcgag
     4261 cgtgttccag gccaagaagt ccctgcaaat caggtgacgt ctgttttcgc tccaggacaa
     4321 aggccagttg ctgatcaagg tcgagcgacc gcaagtcatc aggagtgacg ccaagcaatg
     4381 gatccaccag tgaagccagc aacaccgcat tctccacgac tggttcaggc tccggtgtgg
     4441 tgacttccac atcaagcaaa atcagttggg ccacggcttc accctgccgg gtaagctgct
     4501 gggcgacttc ataggcaatc aaaccgccca ttgaccagcc gcagagctga tagggtccgg
     4561 tgggttgaac ttcttttaaa tcagccagat acgctgctgc catggcgggt atgtcttcca
     4621 atggagtcga aacaaaatca attccg
//
LOCUS       DAOQDO010000160         4642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12352_length_4642_cov_2.929366, whole genome shotgun sequence.
ACCESSION   DAOQDO010000160 DAOQDO010000000
VERSION     DAOQDO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_4642_cov_2.929366"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1556)
                     /locus_tag="PKZ53_03670"
     CDS             complement(186..1556)
                     /locus_tag="PKZ53_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39564.1"
                     /translation="MNFRVLCFGLFILFFIGVGTTTACATERLEILAHQAISESQSEA
                     NTAIAALRQAGPAGLDALIDAHRAEINRHLLMTEVQSTPAWDRLSRALDAVSKQRNCY
                     TSQLYWYTDFEAARAAAKASGKPILSLRLLGNLDEELSCANSRFFRTILYANADISEF
                     LRQRFVLHWKSVRPVPKVTIDFGDGRKMERTITGNSIHYVLDSEGRVVDALPGLYGPQ
                     AFLRVLKPAHVAALLASDPSVSSDQRASMLAEYYSRQSKRLLTQWNQDLTTLKVPVSA
                     SGLARLQAGSIPVSTSNPPTARKAAPLAITKSKVEVSLLNVITPDFEQLESATDQQAW
                     KVLAELHCADAKLDERSLRFIRHQGTGACHIPDATNTSGTGESRFERTVQNFERSVAL
                     DTVRNEYLFHTRMYDWLQAGEGENVDQLNERVYAELFLTPSSDPWLGLLPADTYTAIQ
                     NEGIVK"
     gene            1761..2627
                     /gene="uppP"
                     /locus_tag="PKZ53_03675"
     CDS             1761..2627
                     /gene="uppP"
                     /locus_tag="PKZ53_03675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNJ39565.1"
                     /translation="MSLFEAILLGIIQGLTEFLPISSTAHLVIAIKLLHLDTRFSPEQ
                     LTAFEAVIQLGTLAAVLIYFAKDLKTITAAFFIDHLAYFQGKREPGQRALGPSAWLGW
                     YIAIGTVPIVVIGLSFKKQIEGTLTKNLYVIASAMVGLAIILAIAEMVGKRQRSVEHL
                     TWVDAVLIGLAQACALIPGASRSGTTITASLFLGLNRESAARFSFLLSIPAVLAAGLL
                     QFVKEYKYLDRNTLIAVAVSTLVSGISGYAAIAFLIRYLKTHTTSLFIIYRIIVGAGI
                     FLLLALKIIPPI"
     gene            2685..2757
                     /locus_tag="PKZ53_03680"
     tRNA            2685..2757
                     /locus_tag="PKZ53_03680"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2718..2720,aa:Ala,seq:cgc)
     gene            complement(2889..3692)
                     /locus_tag="PKZ53_03685"
     CDS             complement(2889..3692)
                     /locus_tag="PKZ53_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39566.1"
                     /translation="MPGLRFVSRVALCLVVLLCGFSGVSAWAQDLKKVSEEATKLLHK
                     DGLPAAFPCSLDRQPGIGQFLKENKISLNYRADGAIDLVLQKDSNRVLATIDKTVNRV
                     KLGYIYTVERNSTTSMLSSMPMGGNSGGGSEVLLANVNTAICVSLERGSGADAKAAMF
                     TVYKGKLQPLADLMKNIRKTYPQVTVTAEQRAEFQDLPVAQVRFDGSISDFDVQVNGC
                     LVILNRKGWEFSSLKKDASYVLDILPQQNISHQFQLSKGGSGYALMPVP"
     gene            4349..>4642
                     /locus_tag="PKZ53_03690"
     CDS             4349..>4642
                     /locus_tag="PKZ53_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ39567.1"
                     /translation="MSNWKGNLLQYIAAALLITLAAVAPTLAQARATGGAIEGTVTDP
                     TGAVVAGATVTVKNIQNGLEREVTTAEDGVYRIPALDVGTYDISIKVEGFAPQT"
BASE COUNT         1135 a         1153 c         1149 g         1205 t
ORIGIN      
        1 tggtcgttgt ccggggtgtc atatgtttag ggttcagggt tttcggaaga atgaaaaatt
       61 gagaatgaaa aatgaagaaa ttacactaat ttggaatgga atcctcgtat tagaaaacag
      121 tcaagtgatt caattaaata aacttagtga actactgact acgggctact gactacaaac
      181 tggtattatt tgacgatgcc ttcgttttga atcgcggtat aggtgtctgc aggcagtaat
      241 cccaaccatg gatcggagct tggggttaaa aagagttcgg catagacccg ttcgttgagt
      301 tgatccacat tttctccttc accagcctgc agccagtcat acatcctggt gtgaaacagg
      361 tattcgttgc ggactgtgtc gagtgccact gatcgttcga agttttgaac cgtgcgttca
      421 aaacgacttt caccagttcc agacgtgttt gtggcatcag gaatgtgaca ggcgcctgtt
      481 ccctggtgac gaataaaccg caaactccgc tcgtcgagtt ttgcatcggc acaatgcaat
      541 tcagccagta ctttccaggc ttgctggtca gttgcagatt caagttgttc aaaatcggga
      601 gtgattacat tgagcaagga aacttcaacc tttgatttgg taattgccag aggtgctgcc
      661 ttgcgtgcag tcggtggatt gctcgttgag acggggatcg aacctgcctg gagtcgagcc
      721 agcccagagg cggaaacagg aaccttcaat gtggttaagt cttgattcca ctgggtgaga
      781 aggcgtttgg attgacgtga ataatattca gccagcattg atgctcgctg gtcggaactg
      841 acactgggat ctgatgccag caatgcggcg acatgggctg gtttgagtac ccgcagaaac
      901 gcctgtggtc catacaatcc cggcagggca tcaaccacac ggccttcaga atcaagcaca
      961 tagtggatgc tgtttccagt gattgtccgc tccattttgc ggccatcacc gaaatctatc
     1021 gtgactttcg gcacggggcg aaccgatttc cagtggagta caaaccgctg gcgcaaaaat
     1081 tcagaaatgt cggcattggc atagagaatg gtgcggaaaa accggctatt ggcacagctt
     1141 aattcttcat ccaggttccc gagcaaccgg agcgataaaa ttggtttgcc gctggctttg
     1201 gcggcggccc tggcggcttc aaaatcggtg taccagtaca actgtgatgt gtagcagttg
     1261 cgttgtttgc taaccgcatc cagtgctcga ctgaggcggt cccaggctgg agttgattga
     1321 acctcggtca tcagcaaatg acgattgatc tcggctcgat gggcgtcaat aagcgcatcc
     1381 agccccgccg gcccggcctg tcgaagcgca gcaattgccg tatttgcctc agattgactt
     1441 tctgaaattg cctgatgagc aagtatttcc agccgttcag tggcacaagc agtcgtggtg
     1501 cccacgccaa taaagaaaag gataaacagt ccaaagcaca ggacccgaaa attcatagtt
     1561 tttttctccg agggaatttg gcgaagcaat ttgggacagg ctggaaactt cgcgctgtga
     1621 tgcgggctta caccgacgct ttgccaaaaa gttcgtgaaa attttccctt ccagtcagga
     1681 ctgtactatg atcgcgcctt ttggttgggt tgctcattcc cggggcaccc ttcattctgg
     1741 aaagtgaaca ctaaaacccc atgtctcttt ttgaagccat tctcctgggg atcatccagg
     1801 gtctgacgga atttctgcct atcagcagca ccgcccatct ggtgattgcg attaaactac
     1861 tccaccttga tacccggttt tccccagagc aattgactgc atttgaagcg gtgatccagc
     1921 ttggcacatt ggctgcggtt ttgatctatt ttgccaaaga cttgaaaacc atcacggctg
     1981 cttttttcat tgaccatctg gcctattttc agggaaaacg tgaaccggga cagcgagcgc
     2041 ttggtccgtc agcctggctc ggctggtaca ttgcaattgg aaccgtgccg attgtggtga
     2101 ttgggctctc gtttaaaaaa cagattgaag gcacactcac caaaaacctg tatgtgattg
     2161 ccagcgccat ggttggactg gcaatcatcc tggcaatcgc ggaaatggtc gggaagcgcc
     2221 agcggtcagt ggaacacctt acctgggtgg atgccgttct cattggcctg gctcaagcct
     2281 gtgccttgat tcccggcgcc tcacggtctg gaacaacaat taccgccagt cttttcttgg
     2341 ggctcaatcg tgagtcggca gctcgattct cgtttttgct ttcaattcca gccgttctgg
     2401 ctgccgggtt gctccagttc gtcaaagaat ataaatatct ggatcgcaac acgttgattg
     2461 cggtggcggt ttcgacgctt gtctctggaa tcagcggcta tgccgcgatt gcctttctga
     2521 ttcgatactt gaagacccac accaccagcc tctttattat ctatcgaatc atcgtcggag
     2581 ctggaatttt cctgcttctg gcactgaaaa taattccgcc aatttagatt tgacaagcgt
     2641 ggggtcggtg cctatcatag cttgctttcc acaatgatct ttttggggca gtagctcagt
     2701 tggcagagcg ccgcgttcgc aatgcggagg tcaggggttc gacccccctc tgctccattc
     2761 aaagcaaaaa taaaaaacca gctcaccaga gctggttttt tattttctca atagtttttc
     2821 acccctcagc ccattttctt cagccccgag cttgcgagtc ttcagcccgt cttcttcagc
     2881 ccactgcttt aaggtactgg catcagcgca tagccgctcc cgcccttgct caactgaaac
     2941 tgatgcgaga tgttttgttg tggcaaaatg tcaagcacat aggacgcatc tttcttcaag
     3001 gatgagaatt cccagccctt gcggttcaaa atcaccagac agccattgac ctgcacatca
     3061 aagtcgctga ttgatccatc aaaccgaacc tgagcaaccg gtaaatcctg aaattcagcc
     3121 cgttgttcag ccgtgaccgt cacctgcgga taggtcttgc gaatattttt catcaaatcg
     3181 gccagtggct gcagttttcc tttatacacc gtgaacatag ctgccttcgc gtcagcgcca
     3241 ctacctcgtt caagcgatac acaaatcgcc gtattcacat tggcgagcag gacttccgat
     3301 ccgccccctg agttcccgcc cattggcatt gatgacaaca tcgaggtcgt cgaatttcgc
     3361 tcgactgtat agatataccc taatttcacc cgattgacgg tcttatcaat cgtcgccaga
     3421 acgcgattgc tgtctttttg caaaaccaga tcaattgctc catcagctcg atagttgagg
     3481 gaaattttat tttctttgag aaattgccca attcctggtt gacgatcaag ggaacagggg
     3541 aaagctgcag gcaagccatc tttatggagc agtttggtgg cttcttcgct gactttctta
     3601 aggtcctgcg cccaggcaga aacacccgaa aaaccacaga gcaacacgac cagacacaag
     3661 gcaacgcggg aaacaaagcg gagacctggc atagatcaac ctccttctgc aaagtcaaga
     3721 acagcagagt tgagtgtaag cgtggatggg ggtagggtga tcaggattgg atcaatcgtt
     3781 gagggagttg taattgacta cgagatacat tattgcctgt ttggcatcga gttgtctagt
     3841 caccaacttc aaaaatctga agaataactg aaaaaaccca atcaggtcat ttatctacgg
     3901 acttgcgttt cactggaagg gcactataat tatccctatc tacacaccct tcaaccaggt
     3961 tccatggtta ctccagagat caacttcaat tctattctca atcccactgg aggatctcat
     4021 tctcgccaaa actgctgaaa ttgtctgaag tttaagccaa atttacacgt gtctggtaac
     4081 taaacgctct ttttgcgacg caggccgaaa aagcgtcttt ccagtcaaga ttgttttcaa
     4141 cgtgcgcggt gggtgtcagc acttctcggc atagttcaca aacaggttgg ctgaaaagaa
     4201 cctctcgact gcatcgagtt tatccgcgat tgtaccggct ttctcaggcg ttatgcctgg
     4261 ttgaccatac ggcaacgagc ggaattcatt tatagggccc tatcaatcaa ttagaggccc
     4321 atatctaacc caggaaggga ctctctgtat gagtaactgg aaagggaact tactgcagta
     4381 catcgctgct gcgcttttga ttacgttggc agcagtagct ccgacgttgg cgcaggcacg
     4441 tgccactggc ggggcgattg aaggaacggt gacggaccca accggtgctg tggtggccgg
     4501 cgccaccgtc acggtcaaaa acattcaaaa cggtttggag cgtgaagtta caaccgccga
     4561 agatggggtg taccggatcc cggcacttga tgtcggaacc tatgatatct caatcaaagt
     4621 ggagggcttt gcacctcaaa cc
//
LOCUS       DAOQDO010000161         4638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12365_length_4638_cov_3.248964, whole genome shotgun sequence.
ACCESSION   DAOQDO010000161 DAOQDO010000000
VERSION     DAOQDO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12365_length_4638_cov_3.248964"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..3894)
                     /locus_tag="PKZ53_03695"
     CDS             complement(13..3894)
                     /locus_tag="PKZ53_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39568.1"
                     /translation="MLSKVKIMLGGGLIAGWLVLSGGLAGSVTPPSVQAAGSPQTKSA
                     STQNPSSLPVLKSKSTTLKGAEKHRYQIVIQANDYLNLVVDQQGIDVVVRLFQPDGKL
                     VQEVDSPNGMFGPEPIYVILDRAGTYVLEIESLEKEAQSGKYEVVLDPVHSATEQDRV
                     LIEVQRLQSEARIGMRTGKNDLALTAAQRGMELAKKSGGADSILCANGLEVLGKVYVA
                     KGDYDQAEVHLAKSIETYDKVLKNDDLRSTSCMIELANLYVIKNQIERAESLFQRCLA
                     LREKVLGPDHPEVGDALSWLGSVYIGQADYSKAEPLLVRSLAINEKTVGDSQKTALSL
                     IGLGVMHHLRGEYEKARSYYERSLAVTEKIVGPEHPSTLQALGNLAFLSMQQGDYLNA
                     ESYFLRVISIQEKNLGPIHPDLAITLSNLGQLYKTMANYDQAEASYQRSLAINEKIMG
                     PTHPWVALTLNGLAQLQVEKGEYPKAEKFCQQALAINEKAYGPTHPEVAANLNNLGDL
                     YRRMSNFSAAEPLYRRAVMIWEKALGSDHPNVASGLSNQGTLYLEMGNLDQAEACYRK
                     ALAIQEKKLGPNHSQVGLLLGNIAKVLTRKGDYAASRQLQEQALAIREKALGPDHPEI
                     ASSCGDLGIALTYLGTYDLAEAMFTRALTIYQKKFGPGHRGVALSFKNLGLLHRIQGN
                     FTRAEEFYRKALALQVENLGSSHIETVTCLGALALVQQGLGHLDQAVDSQFQFSEACE
                     RNLAHNLVVGSERQKALFINSTADYADATVSLHLNAAPQNPAAAKLALTEVLRRKGRA
                     LEVMTSSLATLRRQTDPETQKLLDDYAALTNQISILTLKGPGTLPVEKHRAYLNQLTA
                     RQEQLEADLTRRSAEFAVTTNSITIDAVRAMIPDQAVLVEFTVYHPFNPKANRSEPAH
                     YGVYVLTHQGDIRGVDLGETGPIDAAIAQFRKAVRNTQMKVGSSRNLAKVKQNQLVTE
                     TVMAWARQVDRLVLEPVRAFIGTRKHLVISPDGDLNLIPFAALVTESGKFLVEDSLIT
                     YVTSGRDLLRMQVKIPNQQPALIVADPDYAEGKGPTLGGRVMKPLMLLPGTRREGKDI
                     QNLMLGSRLVTQGAATKEVVQQTHRPEILHIATHGYFLEATTRDRSEAGTRVLERFEF
                     QEENPITIQNPLLRSWLFFAGANREESTEKSAIMTALEASQLDLWGTKLVVLSACDTG
                     VGEVKNGNGVYGLRRALVLAGSESQMMSLWSVSDVGTRELMVEYYTRLKAGEGRSEAL
                     RNTQLKMLKDPRRQHPFYWASFIQSGEWKKL"
     gene            complement(4070..>4638)
                     /locus_tag="PKZ53_03700"
     CDS             complement(4070..>4638)
                     /locus_tag="PKZ53_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ39569.1"
                     /translation="LERNECDVCDLILELLNDLSGFLKKRRQILALDLPESVILRADR
                     EKLRMALVHLLQNAIKFSPDGAQIQLRVTCLNEQVHFEIRDSGIGIAPAELERIFEFF
                     YTGADPATHSSGKYEFSARGNGLGLSIARSYVAAHGGRLWAESDGPGKGSCFHLELPL
                     AEPQVTVGSENKTLSKLDETVHNSPSCP"
BASE COUNT         1040 a         1279 c         1128 g         1191 t
ORIGIN      
        1 atgttttctg ccctaaagtt ttttccattc ccctgactga ataaagctgg cccagtaaaa
       61 cggatgctgc cgccggggat ctttgagcat tttgagctgg gtgttccgaa gcgcttcgga
      121 acgtccttcc ccggctttga gtcgggtgta gtactccacc atcaattccc gcgtcccaac
      181 atccgacact gaccacaggc tcatcatttg ggattcgctc ccagccagta ccagcgcccg
      241 ccgcaggcca tagacaccat tcccgttttt gacctccccc acccctgtgt cgcaggctga
      301 aaggacgacc agtttggtcc cccataaatc aagctgagat gcctccaaag ccgtcatgat
      361 tgcgctcttt tcggtgcttt cttcacggtt tgccccagca aaaaaaagcc aggatcgcag
      421 caacggattt tgaatggtga tcgggttttc ttcctgaaat tcaaaccgtt ccagaacccg
      481 agtcccagct tcagatcggt ctctggtcgt agcttcaagg aaatagccgt gggttgcaat
      541 gtgcagaatt tcggggcgat gtgtttgttg gaccacttcc ttggtggcag ctccctgggt
      601 caccaaccga cttcccagca tcaggttttg tatgtctttg ccctcacgcc tggttccagg
      661 gagcagcatc agtggtttca tcacccggcc tcccagcgtt gggcccttcc cttcggcata
      721 atcggggtca gccacaatca gcgccggttg ttgatttgga attttgacct gcatccgcag
      781 caaatcccgc ccactggtca cataggtgat caaagaatcc tcgaccagaa atttcccgct
      841 ttccgtcacc agcgcggcaa aaggaatcaa attcaaatcc ccgtcaggtg aaatcacgag
      901 atgtttcctg gtaccgatga aggcccgcac tggctccagt accaaacgat ccacctgtcg
      961 ggcccaagcc atgactgttt ctgtcaccaa ttgattttgt tttacctttg ccaggtttcg
     1021 tgacgagcca accttcattt gggtattgcg cactgccttt cgaaactgtg cgatggcggc
     1081 atcaattggc ccagtctcgc ccaggtccac cccacggata tctccttgat gagtcaacac
     1141 atagacaccg tagtgagcag gttcagacct gttggctttc gggttaaacg gatgatacac
     1201 cgtaaattcc accagtaccg cctggtcggg gatcatggcc cgaacggcgt caatcgtaat
     1261 ggagttggtg gtaacagcaa actcggcgct ccggcgggtg aggtcggctt ccagttgttc
     1321 ctgtcgagcc gtaagctgat tcagataggc ccgatgcttt tcaacgggaa gggtacctgg
     1381 tcctttcaat gtcaatatcg aaatctgatt ggtcagggcg gcatagtcat cgaggagctt
     1441 ctgggtttcg ggatctgtct gacggcgcaa cgtggcaaga ctgctggtca tcacctccag
     1501 cgcccgcccc ttccggcgca acacctcggt caacgccagt tttgcggcgg ctggattttg
     1561 cggtgccgcg ttgaggtgca gcgacacagt ggcatcggca taatcagccg tggagttgat
     1621 aaagagtgcc ttttgccgtt ctgatccaac caccagattg tgagccaggt tgcgctcaca
     1681 ggcttcgctg aattggaact gggaatcaac cgcctggtca agatggccca gcccttgttg
     1741 aaccagagcc agggcgccca aacaggtgac ggtctcaatg tgacttgatc ccaagttttc
     1801 tacctggagc gcgagcgctt ttcgataaaa ctcctccgcc ctggtgaaat tcccctggat
     1861 ccgatgaagc agccccaggt ttttgaaact caaggcaacc ccgcgatgcc ctggtccgaa
     1921 ctttttctga taaatggtga gagcccgggt aaacattgct tccgccaggt cataggttcc
     1981 aaggtacgtc aacgcaatgc ccaagtcccc gcaactgctg gctatttcag gatgatccgg
     2041 tcctaaggct ttttcacgga tggcgagcgc ctgttcctga agttgccgac tggcggcgta
     2101 atcgcctttt ctggttaaaa ccttcgcgat gttccctagc agcaaaccca cctgcgagtg
     2161 attcggtccc aatttctttt cttgaatggc caatgccttt cgataacagg cttcagcttg
     2221 atccaaatta cccatttcga gataaagcgt cccctggttg gacaaccccg acgcaacatt
     2281 agggtggtca gatcccaagg ctttttccca aatcattacc gcccgccggt acagaggttc
     2341 agccgcggaa aaattgctca tccgtctgta taaatcaccc aggttgttga ggttggcggc
     2401 aacttccgga tgggtggggc cataggcttt ttcattgatg gcgagtgcct gttgacagaa
     2461 tttttccgct tttggatatt ctcctttttc cacctggagt tgcgccagtc cattcagggt
     2521 gagagcaacc caggggtgag tgggtcccat tattttttca ttgatggcca gggaacgttg
     2581 gtaactggct tcagcctggt catagttggc catagtttta tagagctgcc ctagattgga
     2641 gagcgtaatg gcaaggtcag gatgaatggg gcccaagttt ttttcctgaa tacttatgac
     2701 tcgtaaaaaa tatgactccg cgttgagata gtcaccttgt tgcatggaaa gaaaggccag
     2761 attcccaagg gcttgcagtg ttgagggatg ttcgggtcct acaatttttt cggtgacggc
     2821 caggctccgt tcataatatg atctcgcctt ctcatattcg ccacgcagat gatgcatcac
     2881 ccccagacct attaaactga gggcagtttt ctgactgtcg ccgactgttt tttcattgat
     2941 cgcgagcgac cgcaccagca acggttcagc ctttgaataa tctgcttgcc ctatatacac
     3001 ggatcccagc caggataacg cgtcaccaac ttcgggatga tccggtccca gtactttttc
     3061 ccggagcgcc agacatcgct gaaatagcga ttcagcccgt tcaatttggt tctttataac
     3121 atataagttt gccaactcaa tcatacagga agttgatcgt aaatcatcgt ttttcaacac
     3181 tttatcgtaa gtttcaatgg acttagccag atgaacctcg gcttgatcat agtcaccttt
     3241 ggccacatac accttcccga gaacttcaag tccgttggca cacaaaatgg agtcagcacc
     3301 accggacttt ttcgccagct ccattccccg ttgagctgcg gtcaaagcca gatcattctt
     3361 tccagtcctc atcccaatcc tggcttccga ctggagccgt tgaacctcta tcaaaacccg
     3421 atcttgctcc gtggctgaat gtaccgggtc aagcaccact tcatactttc cgctttgagc
     3481 ctctttttca agcgattcga tttccaatac ataggtccct gcccggtcaa gaatcacgta
     3541 gatcggttcc ggcccaaaca taccgtttgg gctatccact tcctggacca gtttcccatc
     3601 tggttggaaa agacgaacca caacatcaat tccctgctga tccacaacca ggttcaggta
     3661 atcattggcc tgaatgacta tctgatagcg gtgtttttcc gcgcccttca gagtcgtact
     3721 ctttgatttc agaacaggta gtgatgatgg gttctgagtg ctggcggatt ttgtttgggg
     3781 gctccctgcg gcctgaacac tgggtggcgt cacagacccg gccagccctc cagaaagtac
     3841 taaccaacca gcaataagtc ccccaccgag catgattttc acctttgaca gcattgagcc
     3901 tccgtatttc tcgcacacct caatctgaat ctatcgcact tcactcacaa atttccttgg
     3961 agtgcgggga cttgtcaccg cttttcaaag aaatatttat ctaaaaagct agcgattgat
     4021 ggattgatga attgacggct gtttggtgat tgatttcaga agagatgatc taagggcaag
     4081 atggagaatt gtgtaccgtt tcgtccaatt tggatagggt tttgttttca gacccgactg
     4141 tcacctgggg ttcagccagc gggagttcca ggtggaaaca acttcctttg ccgggaccgt
     4201 cggattccgc ccagagtcgc ccaccgtgcg ccgcaacgta gctgcgcgca atggacaaac
     4261 ccaaaccgtt gccccgcgcc gaaaattcat atttccctga cgaatgcgtt gccggatccg
     4321 ccccggtgta gaagaattca aaaattcgct ccagttcagc cggcgcaata ccaatgccgg
     4381 aatcgcggat ttcaaaatgg acctgttcgt tgagacaggt cacccgaagc tgaatctgcg
     4441 ccccatccgg actgaattta atcgcatttt ggagcaggtg aaccagcgcc attcgcaact
     4501 tttcccggtc agcacgcaga atgaccgatt ccggcaggtc cagcgccagg atttggcgcc
     4561 gttttttcaa aaatccagag agatcattga gcagttccag aatcaaatcg cagacgtcac
     4621 attcattgcg ctccaggg
//
LOCUS       DAOQDO010000162         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12415_length_4628_cov_3.141701, whole genome shotgun sequence.
ACCESSION   DAOQDO010000162 DAOQDO010000000
VERSION     DAOQDO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12415_length_4628_cov_3.141701"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2736
                     /locus_tag="PKZ53_03705"
     CDS             112..2736
                     /locus_tag="PKZ53_03705"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNJ39570.1"
                     /translation="MKPSSKSLLVLFFLSGATSLAYETVFVKLLGYVFGNTTYAVSTI
                     LAAFMGGLAVGSYVFGKYVERGRNGLKLYAYLELGVGLYCAFVPLIFRGLEPIYVRLY
                     QNYHFSFGQLTAVRFLLSLVLVLPPTILMGGTLPAMTKYLVQHAGHIGRRVSLLYAVN
                     TFGAAFGVFSVTFFILGALGIYGTLGLAVVGNLIIFGIALWLLRGASQVEPVTGTIPV
                     PVSEDLPSWPVKKLEHGFDALASWLGFRPTESETLPASSSSALHRWLYVVAFLSGVIS
                     FAYEVAWTHVLAQTVGTSAYAFGTMLTTMLLGIALGSHFVKQVIDEHKSAECRLVLSQ
                     FALGVAVFVLLPLWDDIPRLFQLTEFFRPSFTLTESIRFIGCFTVMFLPALLIGMSFP
                     LVMKVQARKLPGLAEQIGIVYFFNTIGCIFGSTVTGFVLLPYLGAERLMRVSAAANLG
                     LAVLIAVVFLSQHKQFLRRFVVLTLAVGVLGFTIIPKWDLNHLNVGAHVLSSYTYANP
                     LNQKILFYHEDVLGGVTSVVQSDTTRTLLANAKFQGNDTHEVPAQKQFAIVPLLFTRN
                     YNQSLVIGLGTGTTASVIGAFPFSEIDIAEISPGIVTAAHDYFGHLNRNILDDPRVKL
                     HVTDGRNYVLLTPKQYDLISIELTNIWFAGAANLYSQNFYELCRKRLRSGGVFQQWVQ
                     IHHMDPVDLISIVKTMHSVFPHMAFFVGGGQGILLGSDQPLEVNFKTLESLNERPQIH
                     QCLDNASIVGNDMLSVLSGLYLYDQSVVEFINSEPMAEISTDWYPFLEYTTPRGNQLP
                     YSRLANLKLLESYWYRKLPRLTDATEADMLYLQGLVETGRGNHAQAVRWLEQALAKGN
                     QNPRCSELLRKEREALVK"
     gene            complement(2800..3918)
                     /gene="thiO"
                     /locus_tag="PKZ53_03710"
     CDS             complement(2800..3918)
                     /gene="thiO"
                     /locus_tag="PKZ53_03710"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNJ39571.1"
                     /translation="MKTTDVIVIGGGVIGLAIGRALRQAGLQVTLFERNDQPGGEASW
                     AAAGMLAPQSEATGPGAMLDLCLASRALYPKLAECLRDETGIDIELRQEGTLLPSFSA
                     DQHTQLQAFVNWQQSAGLRASLISAAEAFEHEPHLSPKLVEAVWLPDDWQVDNRRLTR
                     ALITAAYLAGVDIRCNAGSVQLLAEHNRVKGVEVGLERWSAPVVINAAGSWAAQMPVE
                     LLRLPRASVRPIRGQMLALEMQPPTLLRHVIRSEAAYLVPRRDGRLVVGATVEDTGFA
                     KHVTARGVMALLKGVIETVPALADCALIETWAGLRPLAEDQLPILGATDVSGLLCATG
                     HYRNGILLTPITASVMTSLVTGTAPAVDLTPFSPQRFR"
     gene            complement(3955..>4628)
                     /locus_tag="PKZ53_03715"
     CDS             complement(3955..>4628)
                     /locus_tag="PKZ53_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39572.1"
                     /translation="FHTLAVDHCLNVISPRALTAGAISEAVLAAIEETNSFVVNMINE
                     NGWGKAGSTIVTAVIWESWVIAMNLGDSPLFHYQPRTRAFTKITEDHSVAAALARAGV
                     IPPDMIQAHEGRSQLQYFLGGTAFPRNYGPIVFRLESDDVLLLCSDGICGKVPEQSLA
                     EIVGQRNLHLGVIADRLIQASGETGETDNQTLILWRCTLTEPVVPGGGQTMVLGSPRV
                     EDDTE"
BASE COUNT         1069 a         1246 c         1149 g         1164 t
ORIGIN      
        1 aaataactca aatagaacca aatacacttg acacaaactc tcagttccca acgctcgact
       61 actgactact gactctaaac ttgtatcagt gaccgctctt ccctcctcat catgaaacct
      121 tcctcaaaaa gcctgctggt gttgtttttt ctttcgggtg ccacgtcact cgcctacgaa
      181 acggtttttg ttaaactcct tggctatgta tttggaaata caacctatgc cgttagcacc
      241 attctcgcag cgtttatggg cggattggcg gtgggcagct atgtttttgg gaaatatgtc
      301 gaacgcggaa gaaatggatt gaaactgtat gcctatctcg aacttggggt tggcctgtac
      361 tgcgcctttg tgccattgat ctttcgcggt ctggaaccaa tctatgtccg gctgtatcaa
      421 aactaccact tctcgtttgg gcaactgacg gcggttcgat ttctgctctc gctggttctg
      481 gtcctgccgc caaccattct catgggaggc acattgccgg cgatgaccaa ataccttgtc
      541 cagcacgccg ggcacattgg acggcgggtc agcctgctct atgcggtcaa cacttttggc
      601 gctgcgttcg gagtgtttag cgtcacgttt tttatcctgg gagcgcttgg catctatgga
      661 acgctgggtc tggcggtcgt gggaaatctg attatttttg gtattgccct gtggctattg
      721 cggggagctt ctcaggttga accggtgacc gggacaattc cagtgcccgt ttcggaagat
      781 ttgccttcgt ggccggtcaa aaagttggag catgggtttg atgcgctggc gtcatggctt
      841 ggatttcgtc caaccgaatc agaaacactt ccggcaagtt ccagttcggc gctccatcgg
      901 tggttgtatg ttgttgcctt tctttcaggc gtgatttcat ttgcctacga agtcgcctgg
      961 acgcatgtcc tggctcaaac cgtcggcacc agtgcctatg cctttggaac catgctcacc
     1021 acgatgctgc tgggaattgc gttgggaagc cactttgtca aacaggtgat tgacgaacac
     1081 aaaagcgccg aatgtcggct ggtgctctcg caatttgcgc ttggggtggc ggtatttgtc
     1141 ctgctgccac tctgggatga tattccaaga ctgtttcaac tgactgagtt tttcagacca
     1201 agttttaccc tgactgaatc aatccgcttt atcgggtgtt tcacggttat gtttttgccg
     1261 gccctgttga ttggcatgag ctttccgctg gtgatgaaag ttcaggcacg aaaactgcca
     1321 gggttagctg aacaaattgg tatcgtctat ttcttcaaca ctatcggatg tattttcggt
     1381 tccacggtga ccgggtttgt gctccttccc tacctcgggg ctgaacggct gatgcgagtc
     1441 tcagcggcgg caaacctggg attggcggtc ctgattgcgg ttgtcttcct gagtcagcat
     1501 aaacaatttc tccgccgctt tgtggtattg accctggcag tgggagtact gggttttacc
     1561 atcattccca aatgggattt aaatcacctc aacgtcggcg cccacgtcct gagcagctac
     1621 acgtatgcca acccattgaa tcagaagatt ttgttttatc acgaagatgt acttggcggc
     1681 gtcaccagtg tggttcaaag cgacaccacg cgcacactgc tggccaatgc caaattccag
     1741 ggtaacgaca cgcacgaagt tcccgctcag aagcaatttg ccattgtgcc gcttttgttt
     1801 acccgtaact ataaccagtc actggtgatt ggacttggaa ccggcaccac ggccagcgtc
     1861 atcggagcgt ttcccttttc agaaattgat atcgctgaaa tttcgccggg tattgtcacc
     1921 gccgcacacg actattttgg gcacctgaac cgcaacatcc tggatgatcc gcgagtcaag
     1981 ctccacgtca ccgatgggcg caactacgtt ttattaaccc cgaaacaata tgacctcatc
     2041 agtatcgaac tcaccaatat ctggtttgcg ggcgcggcca acctgtacag ccagaatttt
     2101 tatgagcttt gtcgcaaacg gcttcggtct ggaggtgtgt ttcagcaatg ggttcagatt
     2161 caccacatgg acccggtgga tttgatcagt attgtcaaaa ccatgcattc ggtatttccg
     2221 cacatggcct tttttgtcgg aggcggtcag ggaattctgc ttggttcaga ccaacccctg
     2281 gaagtcaatt tcaaaaccct tgaatcactc aatgagcgcc cacagatcca tcaatgtctg
     2341 gacaatgcca gtattgttgg aaatgacatg ctgtcggttt tgagcggcct gtatctctat
     2401 gaccagagtg ttgttgagtt tatcaatagc gagccgatgg ctgaaatcag cactgactgg
     2461 tatccatttc ttgagtacac cacgccacgt ggaaaccagt tgccctattc acggctggcc
     2521 aatctaaagt tactggaaag ttactggtac cgaaaactcc cacgcctcac agatgcaacc
     2581 gaggccgata tgctttattt gcaagggtta gtcgaaaccg gacgtggcaa ccatgcccag
     2641 gcagtcaggt ggttggaaca ggcgttggct aaaggaaatc aaaatccacg ctgcagtgag
     2701 ttattgcgga aggaacgtga ggcactggta aagtaagggt cagggttttc gaggcttatg
     2761 ctttgtatct ctttgaagca atggactttt tctctggttt taccgaaatc gctgtggtga
     2821 aaatggcgtc agatcaaccg ccggtgcagt accagtgacc aacgatgtca tcaccgaagc
     2881 ggtaatcggt gtaagcaaaa ttccgttccg ataatgcccg gtggcacaga gtaaccctga
     2941 aacgtccgtg gcccccagaa tcggcaactg atcttcagcc agtggcctca acccggccca
     3001 ggtttcaatc aacgcacaat cagccagtgc cggaaccgtt tcaatcaccc ctttgagcaa
     3061 ggccatcacc cctctggcag tcacgtgttt ggcaaagccg gtatcttcaa ccgtggcgcc
     3121 gaccaccagc cgtccatcac gccggggaac aagataggcc gcttcagatc gaatcacatg
     3181 ccgcaagagt gtcgggggtt gcatctccaa agccagcatt tgtccccgaa tcggtcgcac
     3241 actggctctg ggcaagcgga gtaattcgac tggcatctgg gcagcccaac tccctgcggc
     3301 gttaatcacc acaggtgctg accagcgctc aagccccacc tcaactcctt tcactcgatt
     3361 atgttcagcc agaagttgca cacttccggc attgcagcgg atatcaactc cggccagata
     3421 ggcagccgta atcagcgcac gcgtcagacg gcggttatca acctgccagt cgtcaggcag
     3481 ccagacagct tcgaccagtt tgggtgacag atgtggctca tgttcaaagg cttcagcggc
     3541 ggaaatcaga gaggcccgca acccggcaga ttgctgccag ttcacaaaag cctgcagttg
     3601 ggtatgctga tcggctgaaa atgaaggcaa gagtgtgcct tcctgccgca gttcgatatc
     3661 aattccggtt tcgtcacgca aacactcagc cagtttggga tacagcgccc ggctggccag
     3721 acacaaatca agcattgctc ctggccccgt ggcttctgat tgcggcgcca gcattccggc
     3781 agccgcccag gaggcttcac caccaggctg gtcattgcgt tcaaaaagcg tcacctggag
     3841 cccggcctgt cgtaaagcac gaccaatcgc cagaccaatg acccctccac caatcacaat
     3901 tacatcagtt gtcttcattc tttcttatac ccacctcaag gcggaatgct caacttattc
     3961 ggtgtcgtct tcgacacgtg gcgagccaag caccattgtt tgcccacctc ctggaacgac
     4021 aggttcagtc agcgtgcacc gccataaaat caacgtttga ttgtcagttt caccagtttc
     4081 cccgctggcc tgaatcaacc tgtcagcaat aaccccaaga tgcaaatttc gttgtcccac
     4141 gatttcagcc agcgactgct ctggaacttt gccacagatg ccatccgaac aaagcaacag
     4201 cacatcgtct gactccagtc gaaagacaat cggtccatag ttacggggaa aggctgttcc
     4261 gccaagaaaa tactggagct gacttcgacc ttcatgcgcc tgaatcatat ctggcggaat
     4321 caccccggca cgtgccagtg ccgctgcaac cgaatgatct tcagtgattt tggtaaaagc
     4381 acgggtgcga ggttggtaat gaaacagagg gctgtcacct aagttcatgg caatgaccca
     4441 actttcccag atgacggcgg tcacaatcgt ggaaccagct tttccccagc cattctcatt
     4501 gatcatgttc accacaaagc tattggtttc ttcaattgct gccaaaactg cttctgaaat
     4561 ggccccagcc gtgagcgcac gaggtgaaat gacatttaag caatggtcaa ctgccagagt
     4621 atgaaata
//
LOCUS       DAOQDO010000163         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12439_length_4622_cov_3.046639, whole genome shotgun sequence.
ACCESSION   DAOQDO010000163 DAOQDO010000000
VERSION     DAOQDO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12439_length_4622_cov_3.046639"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKZ53_03720"
     CDS             <1..899
                     /locus_tag="PKZ53_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ39573.1"
                     /translation="DHQIRAFSNVCRHRAGPVSTGAGSCSTFRCGYHGWSYALDGRLI
                     GTPEFDGVECFQKEAFRLPEFQVATWFGLVFVNCDPQAAPLDEYFDGIADRIIPFDVE
                     NMRYAFRKDWYVDCNWKVYVDNYLEGYHIPIVHPGLNKELDYAQYRTETRRYYSIQHS
                     PVKNANRIRVTGNLATDRAEYFWIFPNLMLNVYPDNFSTNLIIPLGPDKTLTIFEWFF
                     LDPEEPHVQEKLQQQVEFSDEIQIEDIHICEAVQRGLKSKTYTSGRYSVKRENGVHHF
                     HQLLGEFLFQKTPAHSLSKGNS"
     gene            904..1437
                     /locus_tag="PKZ53_03725"
     CDS             904..1437
                     /locus_tag="PKZ53_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39574.1"
                     /translation="MSIPNEELLTAFKTILDSSLGAMEERFNQRLDAVEHRLDAKIDG
                     VHAELTAKNDSLRSEMIERFDFLDQKIDRVQTELSEKFELLQAQIDALRKDLDYKIGL
                     AMAMAKDLSRNGKELKASLQNIEQDLKILGRRPLEAQSDATRALAQNTQLFDQIEQLE
                     RRFRTLEQQFKALTHKK"
     gene            1617..>4622
                     /locus_tag="PKZ53_03730"
     CDS             1617..>4622
                     /locus_tag="PKZ53_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39575.1"
                     /translation="MRKQTFERLILLVVLCLCPLISDVMWAQAYRGSIVGIITDQTGG
                     TLAGATVTAVNVSTGIERSTITADDGSYRIPELAIGTYEIRVEQSGFKSATVTNVVVT
                     VASQQRVDAALQTSSTVSDTIVVEGSVPLVDTTTNVLGTVLQNKQISELPVNGRDFQK
                     LLIISAGASGDPSGAADSPGSFGLFSSNGNRGRSNNYLLDGTDMNDGYRNLPAINQGG
                     VFGVPATVLPIEAISELVVLSNFAPEYGRNSGAVVNIVTKSGTNEFHGSAFEFFRNNA
                     LDARNFFNTKPNPQTALRNNQFGFAVGGPIVKNKTFFFVNYEGQRERVGLNSLARVPD
                     PREIASLGGATNPIIQQILNRNPWPTPNISVPLFDPSPNASVTTPAFADSDSVIAKID
                     HSFTTNDQISGRYYFGDSEQSFPLAIIGGNVLPGYNTNTPTRVQVLSLSYLKIISSSL
                     VNELRYGYNRYNQGFFAQDNDFDPNSIGLNTGVTNPRDFGLPFIRIRNDERLGSAIAP
                     LGATLSVPRGRIATNNQIIDNVSWKLNKHDLKFGYEFRRTFVNGFFDAGYRGRIDFAS
                     LEDFLAGRPSGGRSASGDSQRGTFQNAHGLYLQDSFRLTPKVTLNLGVRYDYFGVLGE
                     ERDRLSNFDSQRGLFLADRLYDRDPNNFSPRLGVAWDVTGKGKTVIRAGWGIFYDSFS
                     QDFFVGQLPFNTFNPGPAYNPIGESPILFSFSAVDRLVPGSPVFSEFLDSDIFAVDSN
                     LRTPYVQNFNLNFQQELFKNTVLQVGYVGSQGRKLYRYRDINQPGNPAVNPGRPFDNG
                     PFAPSGGTFFYVNQFESTAASNYNSLQTTLTMRELRGWNVSVNYTWSHAIDNASDGQD
                     FVPNATQPDNSFRPDLERGNSNYDSRHRFVVSFVYQLPKFDVTNRFMNKMVNGWQMSG
                     LITVRSGNPFHVNFFDDFNGTGEFFPRPELVGDPYAGTSDPDRFLNLAAFQVPCQLDE
                     NGACIPGTQQFGSLGRNSLRGPNYRNFDFS"
BASE COUNT         1154 a         1222 c         1109 g         1137 t
ORIGIN      
        1 atgaccacca gattcgggct ttttccaatg tctgtcggca tcgcgccggg ccagtttcga
       61 ccggcgccgg gtcgtgttcg acctttcgtt gtggctatca cggctggagt tatgccctgg
      121 atggtcggct gattggaaca cccgaatttg acggtgtcga gtgctttcaa aaagaagcct
      181 tccgcctgcc tgaatttcaa gtggcgacct ggtttggact ggtgtttgtc aattgtgatc
      241 cacaggcagc cccgctggat gagtactttg acggcatcgc cgaccggatc attccatttg
      301 acgtcgaaaa catgcgctat gctttccgca aagactggta tgtggactgt aactggaagg
      361 tctatgttga taactacctt gaaggctacc acatccctat cgtgcatccg ggattgaata
      421 aagaactcga ttatgcccag taccggactg aaacccggcg ctattattca atccagcatt
      481 caccggtcaa aaatgccaat cgaattcggg tcacgggcaa tctcgccaca gatcgagctg
      541 aatatttctg gatttttccc aacctcatgc tcaatgtgta ccctgataat ttctccacca
      601 atttgatcat tccccttggt ccagataaaa cacttaccat cttcgaatgg tttttccttg
      661 atccggaaga accacacgtt caggaaaaac tccagcaaca ggttgaattt agcgacgaga
      721 tccagattga agacattcac atctgcgaag cagtgcaacg tgggttaaag tccaaaactt
      781 acacgagcgg acgatattcg gtgaaacgtg aaaacggggt gcatcacttt caccagctcc
      841 ttggtgaatt tctctttcaa aaaacaccag cgcactctct ttcgaaaggc aattcataaa
      901 cctatgagca taccgaatga agaattactg acggcattca agacgatcct cgattcatcg
      961 cttggagcga tggaagaacg tttcaatcaa cgactcgacg ctgttgagca ccggcttgac
     1021 gccaaaattg acggtgttca cgctgaactc acggctaaaa atgacagtct tcgatctgaa
     1081 atgattgaac gctttgattt tctcgatcaa aaaattgacc gtgtccagac tgaactttct
     1141 gagaaatttg aattgctgca ggctcaaatt gacgcactgc ggaaagacct tgattacaaa
     1201 attggtctgg cgatggcgat ggccaaagat ctttcacgca atgggaaaga attaaaagcc
     1261 tcacttcaga atattgagca ggatctcaaa attcttggcc gtcggcctct ggaagctcaa
     1321 tctgacgcaa cacgagcact cgcccaaaac acgcagcttt ttgaccagat tgaacagctt
     1381 gagagacgct ttcgcaccct tgaacaacaa ttcaaagctt taactcacaa aaaatgaaga
     1441 atgaagaatg aggaattgag aaagtgcctg tcgcttagga tccaatggtt tccaagcacg
     1501 aagaaccaag aatgaagaat caagtaccag ttttcttcat tcttcattct caattctttc
     1561 agcttcttca attcaaaaaa gctccacccc cctaaaacgt aaggagtaat tttttcatga
     1621 gaaaacaaac ttttgagcga ttgatactgc tcgtggttct ttgtctgtgc cccttgatct
     1681 cggatgtgat gtgggcccag gcatatcggg gatccattgt ggggatcatt accgatcaga
     1741 ctggcggcac gctggctggc gccaccgtaa ccgctgtaaa tgtcagtacg ggcattgagc
     1801 ggtccaccat cacggcggat gatggaagct atcgaatccc ggaactggcg attggaacct
     1861 atgaaattcg ggttgagcaa tccgggttta aatcagccac cgtgacgaat gtggtggtga
     1921 ccgttgccag tcagcaacga gtggatgccg ctcttcaaac cagcagtacc gtctcagata
     1981 cgattgtggt cgaagggtct gtcccgctgg tagacaccac caccaatgtg ctgggaacag
     2041 tgttgcagaa caaacaaatc agcgaactcc ctgtcaatgg gcgcgatttt caaaaacttc
     2101 tgatcatcag cgccggggca agcggtgacc caagcggtgc ggctgattcc cctggttcat
     2161 ttgggttgtt tagttcaaac gggaaccgtg gccggtccaa caactatttg ctggacggaa
     2221 cggatatgaa cgatggttac cgcaatttgc cagccatcaa ccagggcggg gtgtttggtg
     2281 tcccagccac ggttctgcca atcgaagcaa tttcggaact ggtggtgctg tccaattttg
     2341 cgccagaata tggtcgcaac tcaggggccg tggtcaatat cgtgaccaag tcaggcacca
     2401 atgagttcca cgggagtgca tttgaattct tccgcaataa cgcgctggat gcccggaact
     2461 tttttaacac caagcccaat ccgcaaacgg cgctccgcaa taaccagttt ggatttgcgg
     2521 tcggcgggcc gattgtcaaa aacaagacct tctttttcgt caactatgaa ggtcaacgcg
     2581 agcgggttgg cctcaacagc ctggcccgag tgccagaccc acgtgaaatc gcttccctgg
     2641 gtggtgcgac caaccccatc attcaacaaa tcctgaatcg gaatccgtgg ccaacgccaa
     2701 atatctcagt accgttgttt gatccatcac cgaatgccag tgtcaccacg ccagcctttg
     2761 ccgattctga cagcgtgatt gccaaaattg accattcctt taccaccaat gaccagattt
     2821 caggccgcta ttatttcggt gatagcgaac agagcttccc actggccatc atcggtggaa
     2881 atgtcctgcc gggctacaat accaataccc caaccagggt tcaggtcctg tcactgtcct
     2941 acctgaagat tatctccagc agtctggtta atgaattacg atatggctat aaccggtaca
     3001 accagggctt ttttgcccag gacaacgatt ttgacccaaa ttcaattgga ttaaataccg
     3061 gagtgaccaa tccgcgtgat ttcggattgc cctttatccg catccgtaat gacgagcggc
     3121 tgggttcagc cattgctccg ctgggcgcca cgctgtcagt cccgcgcggg cgtatcgcca
     3181 ccaataatca gattattgac aacgtttcct ggaaactgaa caaacatgat ttgaaatttg
     3241 gctatgaatt ccgccggacg tttgtcaacg gtttctttga cgctggatat cgtggacgaa
     3301 ttgattttgc ctcgcttgaa gatttcctgg ctggtcgtcc aagcggtggc cggagcgcca
     3361 gcggcgattc acagcgtggc acgttccaga atgcccatgg cctgtatctg caagatagct
     3421 tccgactgac cccgaaagtg acgctcaacc tgggggtccg ctacgactat ttcggagtgc
     3481 tgggtgaaga acgcgaccgg ttgagcaatt ttgattcgca acgtggattg tttctggctg
     3541 accggctcta tgaccgggac ccaaataact tctcaccacg cctcggggtg gcctgggatg
     3601 tgaccgggaa aggcaaaacc gtcattcgcg ccgggtgggg aattttctac gacagcttct
     3661 cccaggattt ctttgttggt cagttgccgt ttaatacctt caatcctgga ccagcctaca
     3721 acccgattgg cgaatcaccg attctcttct cattttcagc cgtggaccgc ttagttccag
     3781 ggtcaccggt cttttcagaa tttctggact cagacatatt tgccgtggac agcaacctgc
     3841 gcacgcccta tgtccagaat ttcaacctca actttcagca ggaactcttt aagaacacgg
     3901 tgcttcaggt tggctacgtt ggttcgcaag gccgaaaact ctaccgctat cgcgacatca
     3961 accagcctgg aaatccagcc gtcaaccctg ggcgaccatt tgataatgga ccatttgctc
     4021 catcgggtgg gacattcttc tatgtaaacc agtttgaaag tacggcagct tccaactaca
     4081 actcactgca aaccacactg accatgcgcg aactgcgtgg ctggaacgtt tcagtgaatt
     4141 acacctggtc acatgccatt gacaatgcca gcgacggcca ggatttcgtt cccaacgcaa
     4201 cccaaccaga caacagtttt cgccctgacc tggaacgcgg caactccaac tatgacagcc
     4261 gtcaccggtt tgtggtgtca tttgtgtatc agttgccaaa atttgacgtc accaatcggt
     4321 tcatgaacaa gatggtcaac ggctggcaaa tgagcgggct gatcacggtc cgaagcggta
     4381 accccttcca tgtcaacttc tttgacgatt tcaacggcac aggcgaattc tttcctcgcc
     4441 cagaactggt gggcgacccc tatgccggca ccagcgaccc ggatcgcttc ctgaatctgg
     4501 cagccttcca ggtgccgtgc cagttggatg aaaacggcgc ctgtattcca ggcacccagc
     4561 agtttgggtc cctgggccgc aattccctgc gcggtccaaa ctatcgcaac ttcgattttt
     4621 ca
//
LOCUS       DAOQDO010000164         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12477_length_4616_cov_3.153694, whole genome shotgun sequence.
ACCESSION   DAOQDO010000164 DAOQDO010000000
VERSION     DAOQDO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12477_length_4616_cov_3.153694"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..2095
                     /gene="bshC"
                     /locus_tag="PKZ53_03735"
     CDS             422..2095
                     /gene="bshC"
                     /locus_tag="PKZ53_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNJ39576.1"
                     /translation="MTAPSLVSTDSVCFFTHHQEFPGNSRLFLDYLHGSDQLAAFFPG
                     FSSQILSVQHLATLAPTIAAEHLERDTLVEVLLEQNRNWGATPHTFAQIDRLRNQQTV
                     AIVGGQQAGLFGGPLLSVYKALTVIHLADQLTHAGTPAVPVFWIASEDHDFAEVNHTV
                     LFGRDGGLVKIPYQDSALSQPPVGKRTIGSSIDQSWEALVAALPETDFTAGILAGLRE
                     AYATGAGWCDAFARWMTRLFSQYGLIVMDPRDARLKTAARQVFEAAISDRQELASAIH
                     RQSQALVEAGYHAQVKTDVNQSLVFVELNETRTSLIQTSEGFQTKSTSGDQHLFSAEA
                     LMAIFESDPLRITPNALLRPVVQDVLLPTVAQVVGPAELAYLAQSQPIYDHLKRTAPV
                     RWPRSGMTLVERRHLKTLEKFQFSLIDVVSGEQTLMRRILTEILDSSTADLFTETEAL
                     FNTQLDKLQASLDRQDPTLAKALAQSREKILYQLNHLQNRFLNSRSQAEDATRRQIER
                     AVVALYPDNGKQEREMNLLPFLARYGWDLVDRLHRELKVDPAHHQWLVV"
     gene            complement(2156..3268)
                     /locus_tag="PKZ53_03740"
     CDS             complement(2156..3268)
                     /locus_tag="PKZ53_03740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ39577.1"
                     /translation="MIDNFASQSDLTNGVSIVIPSFNGQHLLKQFLPSVIEAAEVYEH
                     QSGAPTEIIIVDDGSQDETVNWLTRHFPGRVLCLPQIHNRGFSPTCNIGFAAAQYPLI
                     WLLNNDIEATPTALTRLVSHFQNPDVFAVGSRAYRLSPDLKPTTEIDGAGRVGELVRG
                     FWKVYRSYDLLPEYDRQANQPLFTMMASGGYVLFDATKLKRVGGFEELLAPFYWEDAE
                     LCYRAWKRGWQVLYEPESLVYHQSSATIPKHFEARTVNVIAMRNRMIMHWIHLHDPWM
                     WLLHLGMTGLLLVRDLISGGKYQDLQAFQQVWQMRKKIWQRRQAERQSAIRTDREILE
                     LLKEVTQRQGVMTYKNADEYASFLEKTRQADDNVTR"
     gene            3349..4515
                     /locus_tag="PKZ53_03745"
     CDS             3349..4515
                     /locus_tag="PKZ53_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ39578.1"
                     /translation="MKITFRPLLQKRRLLLAGGALFLVGLLIWHPVSAHFRAARLVVK
                     MAKIAMLDSPILNAHPPIVETILTFPDPQTGHPIRARLYAPQNVTSGPGMVLIHGVHR
                     LGMDEPRLMAFARAFSTTGLMVLTPEITELADYHISPQSIQTIGTTTQFLSQKLGQSQ
                     VGLMGLSFAGGLALLAAGDARYKSSIQYVVSVGGHHDLARVAQFFATNQIPLPDGRIQ
                     KLTAHPYGPLIIVYSNLAHFFPPKDLPQAHEILKLWLWEQFDQAKQQLPSLSPDSQKK
                     MGLLFEGKIDQFAPELLKEIESLRSDMKLVSPNGHLQAVSVPVFLLHGELDPVIPVAE
                     AKWLIKEIPPNPGNHVLISKCIGHVELQGEPSFSEVWQLVHFVAEILSRAGKGG"
BASE COUNT         1108 a         1213 c         1108 g         1187 t
ORIGIN      
        1 gcggttgttc ttcctggacc tgatacccca ttttttccag tgctcgactt aaacgacctc
       61 tcaccgtttc cacagttccc ggcaaccgca atgtgacctc atgttcagtt gggccaacac
      121 catagttgct ggtgtccatt gatagatact cagttcccat aaaccacctg ttcctcaata
      181 agttttcttc tgaaacggag ctccagctcg tggaagttgc gtgatcaact ggtgtgtgat
      241 ttttcaggtc ggcaaagtgg tcaccgattt ttgaaatttt tccgtatgat ggggttcgat
      301 tctgcactcg ccgcgtgaat tcaggctctc aaattttaat aagtttttca acttcaagcg
      361 tttccaaaga cagccgatca cgggtgatga tttcaactct aaccagtcgg agaaaaattt
      421 tgtgacagcc ccatcccttg tttcaaccga ttctgtctgt tttttcactc atcaccagga
      481 attccccgga aattcccgac tttttctcga ttatttacat ggatctgacc agcttgccgc
      541 tttttttccc ggcttttcct cgcagatcct ttcagttcaa catctggcca ccctggcgcc
      601 aaccattgcc gccgaacatc tcgaacgtga cacgctggtt gaagtcttgc tcgaacaaaa
      661 ccggaactgg ggcgccacac cacacacctt cgctcaaatt gacaggttac gcaatcaaca
      721 aacggtggct atcgtcggtg gacaacaagc cggcttgttt gggggcccct tgttgagtgt
      781 ctataaagcg ttgacggtga ttcatctggc tgaccaactc actcacgcgg gaacgccagc
      841 cgttccagtt ttttggattg ccagtgaaga tcacgatttt gccgaggtca accacacggt
      901 cttgtttggt cgtgatggcg gactggtgaa gattccatat caggattccg ccttgtcgca
      961 gcctccagtt ggaaaacgaa cgattggatc cagcattgac cagtcctggg aagcactggt
     1021 ggcggccctt ccagagactg attttacggc tggaattctg gctggtttgc gcgaagcata
     1081 tgcaacaggg gctggctggt gcgatgcgtt tgcccgatgg atgacccggc tgttttcaca
     1141 atatggattg attgtgatgg atccacgtga cgctcgactc aaaaccgcag cccgtcaggt
     1201 atttgaagcc gcgatcagtg accgtcagga attagccagt gctattcacc gccagagcca
     1261 ggctctggtt gaagctggct accacgcgca ggtgaaaacc gatgtcaatc aatcacttgt
     1321 gtttgttgaa ctgaacgaaa cccggacatc tctgattcaa acatccgaag gatttcaaac
     1381 caaaagcacc agtggtgacc agcacctgtt ttcagccgaa gcattaatgg ctatttttga
     1441 atccgacccg ttgcgaataa ctcccaatgc tctgctccgg ccagttgttc aagacgtgct
     1501 gttgccaacc gtcgcccagg tcgttggccc ggctgagctg gcgtatctgg cccaatccca
     1561 gccaatttat gatcatctca agcgaacagc accggttcgg tggccgcgtt ctggaatgac
     1621 gctggttgaa cgacgccacc tgaaaaccct tgagaaattt cagttttccc tgattgatgt
     1681 ggtgagcggc gaacagacct tgatgcgccg aatcctgact gaaattcttg atagcagcac
     1741 ggcggatttg tttaccgaga ctgaagccct gttcaatact cagcttgata aattacaggc
     1801 gtcacttgat cggcaggatc caacgctggc caaagcgctg gctcaaagtc gggaaaaaat
     1861 cctctatcaa ctcaatcatc tacaaaatcg gttcttgaac agtcggtctc aagctgaaga
     1921 cgcaacccga cggcaaatcg aacgggccgt ggttgcgctc tatccagaca atggcaaaca
     1981 ggagcgtgaa atgaacctgc ttccgtttct ggcccggtat ggctgggacc tggttgaccg
     2041 actccatcgt gaactgaagg ttgacccagc ccatcaccag tggctggtgg tttaaaaaac
     2101 aaagtgacag ggtaccacat gagattgagt ctcgcctggt tcccctgtca ccctgtcacc
     2161 tggtcacgtt gtcatctgcc tgcctggtct tttccagaaa agacgcatat tcatcagcat
     2221 ttttataggt catcacaccc tggcgctgag tcacttcttt caaaagctcc aggatttcac
     2281 gatcagttcg gatggctgac tgacgttccg cctgccgccg ttgccaaatt ttcttccgca
     2341 tctgccagac ctgttgaaaa gcctgaagat cctgatattt gccacctgaa atcagatcac
     2401 gcaccaggag caagcccgtc attcccaaat gcaacaacca catccacgga tcatgcaggt
     2461 ggatccaatg catgatcatt cggttgcgca tcgcaatgac attgacggtt ctggcttcga
     2521 aatgctttgg gatcgtagca ctcgactggt gatagaccag actttctggt tcatagagca
     2581 cctgccagcc tcgtttccag gctcggtaac acagttcagc gtcttcccaa tagaatggcg
     2641 ccagaagttc ctcaaacccg cccacacgct tcagtttcgt cgcatcaaag agcacatatc
     2701 ccccgctggc catcattgtg aagagcggtt gattggcctg tcgatcatat tccggaagca
     2761 ggtcataact acggtacact ttccaaaatc ctcgaaccag ttcaccaacc cggcctgccc
     2821 cgtcaatttc ggtggttggt ttcaggtccg ggctgagacg gtaggcccga ctccccacag
     2881 caaaaacgtc tggattttga aaatgtgaaa ccagcctggt cagggcagtt ggtgtggctt
     2941 caatgtcgtt attaagcaac cagatgagtg gatattgggc ggcagcaaac ccgatattgc
     3001 acgttggact gaacccacgg ttgtgaattt gtggcaggca taaaacccga cctggaaagt
     3061 gacgggttaa ccagttgacc gtttcatcct gactgccatc atccacaatg ataatctcgg
     3121 ttggcgcccc agactggtgt tcatagactt cggctgcctc gatgaccgat ggaagaaact
     3181 gttttaacag gtgctgaccg ttaaacgagg ggatgacaat gctgaccccg ttggttaaat
     3241 cggattgtga cgcaaaatta tctatcaaag tggtttagtt gcctgttgag gaatatcatt
     3301 tatgctgtgt ttcagcttaa tttatcatat cctcacacaa ggactgctat gaaaatcaca
     3361 tttcgtccgc tcctgcaaaa acgtcggctc ctgctggcgg gtggggctct gtttctcgtc
     3421 ggattgttga tctggcaccc tgtatcagct cattttcgag ccgcaagact ggtggtcaaa
     3481 atggcaaaaa ttgccatgct cgattcacca atcctcaatg cccatccccc gattgttgaa
     3541 accatcctga ctttcccgga tccccaaacc ggtcatccga tacgagcccg gctgtatgcg
     3601 cctcaaaacg ttacgtctgg accaggaatg gtcttaattc atggcgttca tcggttgggg
     3661 atggatgaac ccaggttgat ggcttttgcc cgtgccttct cgacaaccgg cttgatggta
     3721 ctgacccctg agattacaga actggctgac taccatatca gccctcaatc cattcaaacc
     3781 attggcacaa ccactcaatt tttgagccag aaacttggtc aatcacaggt tggattgatg
     3841 ggactgagct ttgctggcgg attggcactg ctggcagccg gggacgccag atacaagtca
     3901 tctattcaat atgtcgtttc agtcggaggc catcacgacc tggccagagt ggctcaattc
     3961 tttgcaacaa atcaaattcc tttacctgat ggaaggatcc agaaactgac cgcccaccct
     4021 tacgggcctt taattatcgt gtattcaaac ctggcacatt tctttccacc gaaagacctt
     4081 ccccaggccc atgagatcct caaattatgg ctttgggaac agttcgacca ggcgaaacag
     4141 caacttccaa gcctgagccc agactcccaa aagaagatgg ggttgttgtt tgaagggaaa
     4201 attgatcagt tcgcgccaga gctgctcaag gaaattgaaa gtctgcgctc tgatatgaaa
     4261 ctggtatcgc caaatggtca tctgcaggcg gtgtcagttc cggtcttttt actccacgga
     4321 gagcttgacc ctgtcattcc agtggctgaa gccaaatggc tgatcaaaga aattccaccg
     4381 aaccctggca atcatgtctt gatcagcaaa tgtattggac atgttgagct tcagggagag
     4441 ccttcattct cagaagtctg gcaactggtc cattttgtgg ctgagatttt gtccagagct
     4501 ggaaagggcg ggtagggaaa caggtgactg gggcctggtg cctggtgact ggtttttggt
     4561 gcttagtact tagtgcttag aaatcaatac tttcaaagaa tcaccaacca ccaagc
//
LOCUS       DAOQDO010000165         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12533_length_4601_cov_2.818082, whole genome shotgun sequence.
ACCESSION   DAOQDO010000165 DAOQDO010000000
VERSION     DAOQDO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12533_length_4601_cov_2.818082"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="PKZ53_03750"
     CDS             complement(<1..2085)
                     /locus_tag="PKZ53_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39579.1"
                     /translation="MSQIDQQLAQARQLFAQSEFLAAKEHLKEALAAGLDTHHRHQVT
                     PALVLLGQCYLRLKVYPKAVISFEHALKNLTEDEPTLQLLVLNDLALAQSQCLQFDAA
                     LALFEQAIRLALEREQFDVLPSLAYNVCLSLTTLNRTLNKLSDSTLPFAHLKHSLKAL
                     SELVEWLEPSPLSAWLAEIIGTVFFQLGQFDLALSAYQQAQAILNLPELITDQSHERS
                     RRRLIRNLATVFAAVGKTEIAATLLNQVKSWSVEDTSLPAPEFNLPPINPPSIDVVRD
                     LPTLHQLPQGFISLTHESSATGVFLSTYQFHTQDSTKLRWQARGLEFRAELLTDRATT
                     PGEVRHARELLVRALLFWQKLKDPIAQAQCQYRISRLDARLGNVPAAIDHLEQALKLA
                     QTENDLEWELRIRSKLVEILAPQGHVEHLQPHVTRIESRLPCRQPELDALCRFRLGKA
                     AAATGDRERAQTCFEQAIQIIESQRQHIEAENLRSAYLGERISYYAALVQLLLQPAPG
                     ESKTGGTPNQVRSAFEVCERSRARLLRDLMSFGHSQTQLDPLQPKTYRPKPAERPPES
                     EALDPNSHSPELELSITQFHRQQPKLNSRLRNWVELSLFPNGVAAAIQAQLEPESTLI
                     EFFFGDTCFVWVITQDEIRAVPIPNGFELEKLVTQWFDQMTQRTQANPTPNRHEYRKQ
                     LCAADQRGHDLGQ"
     gene            complement(2244..3701)
                     /locus_tag="PKZ53_03755"
     CDS             complement(2244..3701)
                     /locus_tag="PKZ53_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNJ39580.1"
                     /translation="MTATARLFEGQMVQYLESDSPPFGAMKQTFFTPDRSQVIQLYHP
                     ELAAQFTNRLDRLKAIVWKHNLTLPEYAGGATGLTESTARYFKNLFCWPTGIVTEPRL
                     GILAPAYPSNFYFASGPFHGREKELLWFCRTSLRSKLPASEKGDWLRYVQIAILLARA
                     VRRLHQASLIHCDLSSQNILIDPTIGRCLVLDVDSVVVADQFPPDVLGTPRYMAPELI
                     AASAPRQSSIAGLPNFTTDAHSLAVLIYEILLWRHPLMGPKIHSTVSSEEDDFLALGP
                     QALFIENPEDRSNRPHDLEPDVSSLGSSLSTLMYQAFVTGLHQPELRPTAAQFEDGLL
                     DAWDHLHPCLNGDCHHRWFIVTPSEKPRCPFCGAWIFRWVPILEFFLEPLSKQLVPTR
                     SLVVYPELRLFEWHARSSIRPTEDADRTPPGRFLLVENQWFLINDGFDSMVSPGGNPV
                     QPGQAIHLVPGVTFRLNTLPDGWFIRVRSEPIARE"
     gene            complement(3698..>4601)
                     /locus_tag="PKZ53_03760"
     CDS             complement(3698..>4601)
                     /locus_tag="PKZ53_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNJ39581.1"
                     /translation="WMPGGFSRPIQPGDRSGYLDPEVVYLQQSLHLAASEALRAVELE
                     AHRLLTDESFLSPAERSKLTLKDFASTLLLAVHGPIPGPSPATSLVVTCHIGDGMTAV
                     LDTRFSVKLLGIPDEHEPTGKTEFLTTSHRLNPEFLARRTFVYWGDIKGLFMMSDGVA
                     DDYYPMETELLTLFGDLKLNGVLPIQTVEPHQLLNAFNGLPQTVADQIKNVQFASSYE
                     TIAPNPETVYLRSFKELVRKLDLPPATILQSPELIWLATQSGAPPSGETGEASLRKWL
                     DAYQQRGSFDDRTLMVVFPQDPAS"
BASE COUNT         1089 a         1121 c         1323 g         1068 t
ORIGIN      
        1 ctgaccgagg tcgtgtcctc tttggtcagc ggcgcaaagc tgttttcggt attcatgtcg
       61 atttggagtt ggattggcct gggtgcgctg tgtcatctgg tcaaaccact gggtgaccag
      121 tttttcaagt tcaaacccat ttgggatggg aacggcccga atttcatcct gagtgatgac
      181 ccaaacaaag caggtgtccc cgaaaaaaaa ttcgatgagc gtagattccg gttcaagctg
      241 ggcttgaatg gcggcggcca ctccatttgg aaagagggaa agttccaccc agtttcgcaa
      301 ccgggaattt agtttgggct gctgccgatg aaactgtgtg attgacagtt caagttcggg
      361 tgaatgtgaa tttggatcaa gtgcctctga ttctgggggg cgttcagccg gtttaggtcg
      421 gtacgttttt ggctgaagtg gatcgagctg tgtttgcgaa tgtccaaacg acattaaatc
      481 gcgcaacaac cgggcccgtg atcgctcaca gacttcgaag gccgatctca cctggttggg
      541 agtgccgcca gttttgcttt cgccaggtgc cggctggagc aggagctgga ccagcgcggc
      601 gtaataagaa attcgttcgc caagatatgc agaacgcagg ttttcggcct cgatatgctg
      661 gcgctgagat tcaataatct gaattgcctg ttcaaagcag gtttgggccc gttcccggtc
      721 accagttgcc gctgccgctt tgccaagccg aaaccgacac agggcatcaa gttcaggttg
      781 ccggcaaggg aggcgtgatt cgatccgtgt gacgtggggc tgaaggtgct cgacgtggcc
      841 ctgtggggcc agaatctcaa ccagtttaga gcgaattcga agttcccact ctaaatcatt
      901 ttcggtttga gccagcttga gggcctgttc caggtgatca atcgccgccg gtacattgcc
      961 taaccgggca tcaagccggc tgatgcgata ttggcactga gcctgcgcga tagggtcctt
     1021 cagtttttgc cagaacagga gcgcccggac cagaagttca cgggcgtgcc gaacttcacc
     1081 tggggtcgtg gccctgtcgg tgagtaattc agcccgaaac tcaagtccgc gtgcctgcca
     1141 gcggagtttg gtcgaatcct gggtatgaaa ctgataggta gagagaaaca cccccgtcgc
     1201 tgacgattcg tgggtcaggc tgataaatcc ctgcggaagc tggtggagcg ttggtaaatc
     1261 acgaacaaca tcaattgacg gtggattgat tggcggtaaa ttgaactcag gggctggaag
     1321 gctggtgtct tcgactgacc aggatttgac ctgattgagc aaggtggcag caatttcggt
     1381 ttttcccacg gcggcaaaaa ccgtggccag atttcgaatc agtcggcgcc gggatcgctc
     1441 gtgggactgg tctgtgatga gttctggcag gtttaaaatg gcctgggcct gttgataggc
     1501 cgagagtgcc aggtcaaatt gacccagttg aaaaaaaact gttccgatga tttcggccag
     1561 ccaggcactg agcggtgatg gttccaacca ttcaaccagt tcagagagcg cctttaatga
     1621 atgtttgaga tgagcaaatg ggagggttga atccgagagt ttattgagtg tgcggtttaa
     1681 ggtggtcagg gacagacaga cgttataggc cagactgggc agcacgtcga attgctcgcg
     1741 ttccagcgcc agtcgaatcg cctgttcaaa gagtgccagc gccgcatcaa attgcaggca
     1801 ttgtgattgg gccagggcca gatcattgag caccagtaac tggagtgttg gttcatcttc
     1861 agtcagattt ttgagcgcat gttcaaatga aataaccgct tttggataaa cctttaaccg
     1921 caggtaacac tgaccaagca acaccagcgc cggggttacc tgatgccggt ggtgggtgtc
     1981 gagtccggca gccagtgctt ctttcagatg ttctttggct gccagaaact ctgattgtgc
     2041 aaagagttgg cgggcctggg ccagttgctg gtcaatttgc gacaccgatt ctgttcgcgg
     2101 gggccgtttc aaaaatctgg tggaaagccg tttaaagaag ttcatggtga gcgaaagagc
     2161 gaaagagcaa agcattgatt ctaggttcga gtctgtcaac cctttaaatt ctatggtttc
     2221 tattcattga ctattccact cgttcactcc ctggctattg gctcactccg aacccgaata
     2281 aaccagccat caggcagggt gtttaatcgg aacgtgacgc cgggaaccag gtgaattgcc
     2341 tggcctggct gaaccggatt tccgcccgga gacaccatcg agtcaaatcc atcattgatg
     2401 agaaaccatt gattttcgac caggagaaac cggccaggcg gagtccggtc ggcatcttcc
     2461 gtgggccgaa tactggaccg tgcgtgccat tcaaacaacc gcaactcagg atagaccacc
     2521 agcgaacggg ttggaaccaa ctgtttcgaa agcggttcca aaaaaaattc caggatcgga
     2581 acccagcgga aaatccaggc accacaaaac ggacatctgg gtttttctga gggagtgacg
     2641 ataaaccagc ggtggtggca gtctccgttt agacagggat ggaggtggtc ccaggcatcg
     2701 agcaacccat cttcaaattg agccgctgtt ggcctcaatt ccggctgatg gaggcccgtc
     2761 acaaacgcct gatacatcag ggttgaaagt gaactcccca gcgatgaaac gtcaggctca
     2821 aggtcgtggg gccgatttga cctatcctca ggattttcaa taaacagagc ctgaggaccc
     2881 agcgccagga aatcgtcttc ttcgcttgaa accgtggaat gaattttcgg gcccatgagt
     2941 gggtgtcgcc acaggagaat ttcatagatc agaacggcca gcgaatgcgc atcggttgtg
     3001 aagttcggca accctgcgat tgacgattgc ctgggggctg aggccgcaat caattccggc
     3061 gccatatatc gcggcgttcc cagaacgtca ggcggaaact ggtcagccac caccactgaa
     3121 tcaacatcga gcaccaggca ccggccaatg gttgggtcaa tcaaaatgtt ttgcgatgat
     3181 aaatcacagt gaatcaggct ggcttgatgc agtcgccgaa cggcccgcgc cagcaaaatt
     3241 gcaatttgca catatctgag ccagtcgcct ttttcactcg ccggaagttt tgaccgcaat
     3301 gatgttcgac aaaaccagag gagttctttt tctctcccgt gaaagggacc tgaggcaaag
     3361 tagaaattgg acggataggc cggcgccaga atacccaacc tgggctcagt gacaatcccg
     3421 gtgggccaac aaaacaggtt cttaaaatat cgagcggttg attcagtcag tcccgtggca
     3481 ccgccggcat attctgggag cgtcaggttg tgtttccaga caatggcttt gagtcggtcc
     3541 agccggtttg tgaactgagc cgccagttcc ggatgataca actggatgac ctggctccgg
     3601 tctggagtga aaaacgtttg tttcatggcc ccgaaaggtg ggctgtctga ttccagatat
     3661 tgaaccatct ggccttcaaa cagccttgcg gtcgcggtca tgacgccggg tcctggggaa
     3721 acaccaccat caacgttcgg tcgtcaaatg aaccccgttg ttgataggca tccagccatt
     3781 tgcggagtga tgcttcacca gtttcgccag acggcggagc acctgattga gtggccagcc
     3841 agatgagttc aggagattgc aatattgtgg ctggagggag gtccagcttg cgaaccagct
     3901 ctttaaatga tcggaggtac accgtttctg ggttgggggc aatggtttcg tagctggacg
     3961 caaactgaac attcttgatt tgatcagcca cagtctgggg caggccattg aaggcattca
     4021 aaagttgatg cggttcaact gtctggatgg gcaaaactcc attgagcttc agatctccaa
     4081 acagcgtgag caattcggtt tccatcgggt agtaatcgtc ggcaaccccg tctgacatca
     4141 taaacaagcc tttgatatca ccccaataga caaaggtcct tcgagctaga aattctgggt
     4201 ttaaccggtg gctggtggtg agaaattcag tttttccggt tggttcatgt tcatcaggga
     4261 tccccagcaa tttcactgaa aacctggtgt ccagcaccgc cgtcatgcca tcgccaatgt
     4321 gacaggtgac gaccagactg gtggcgggcg atggtccagg aatggggcca tgcaccgcca
     4381 gcaacaacgt tgaggcaaag tctttgagcg tcagttttga ccgctccgca ggtgaaagaa
     4441 atgactcatc ggtgagcagc cggtgggctt cgagttcgac ggcccgcaaa gcttcactgg
     4501 ctgcaagatg gagtgactgt tggagataca ccacttccgg gtcaagatag cctgagcggt
     4561 caccgggctg gattggacgt gaaaaaccgc ccggcatcca t
//
LOCUS       DAOQDO010000166         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12538_length_4600_cov_3.072607, whole genome shotgun sequence.
ACCESSION   DAOQDO010000166 DAOQDO010000000
VERSION     DAOQDO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12538_length_4600_cov_3.072607"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..863)
                     /locus_tag="PKZ53_03765"
     CDS             complement(57..863)
                     /locus_tag="PKZ53_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ39582.1"
                     /translation="MSDSIIPFRFADIQLSTGVRLHYAEQGNPANPPVILLHGYSDSW
                     FSYSQVIDGLAQTAHVFALDQRGHGDSDQPETGYSVPDFAADVVAFMDALNLSSATVV
                     GHSMGSLVAQQVALSAPDRVARLVLIGSATNLRCPEIAELHQAVMTLDDPVPVEFIRE
                     FQVSTIFFPIPDQFLERAITESQKLSANVWHKVITSLLEFDSTAALSRIQSPTLILRG
                     DHDTIFPAPAQQALETGITSSKVKIYPETGHALHWERPEQWLADVQEFLG"
     gene            1149..1556
                     /locus_tag="PKZ53_03770"
     CDS             1149..1556
                     /locus_tag="PKZ53_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39583.1"
                     /translation="MSQFAAGSSPDRSSKFAKISVIIAVVNLGMMVLLLLGVVGSETL
                     RDVFTDERWSKMLRTTSDVCFISLIYYALSIGPLGFLVGISLSIIGTFRTGLKKGMGI
                     FGLVCNFVLFVGVVILDIVIGLAAIGLSALAPR"
     gene            complement(1956..3134)
                     /locus_tag="PKZ53_03775"
     CDS             complement(1956..3134)
                     /locus_tag="PKZ53_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39584.1"
                     /translation="MPYFTRLLPFFLLVWVLIQPIALLADGPQPLSEKEVTQLAAFLK
                     QHWRTPEQYIIEKFKDHDIILLAEDHRIKHNLHLAHNLISLLYRAGVYNFGMEFGASE
                     DQAALDKLVTAEKYDEAQARRLMFHYNVGWAFKEYLDVYRAAWELNRTLPAGARKFRI
                     LNLSYCYDWSEATLVRTPENTKKVFHKGGTEWYRADLIKREILEKKEKILVLTGTPHA
                     FTRYQMPVFDYNARQFYSFENRLLGNLLYKQSPKKVFTILLHQIFESKLNGAMESVYP
                     ARGAIDQVMATFSDKRVGFDLNGTPFGNLADTSFYSTGYDGFHLSQLADGYIYEKPFS
                     EYEACTLDEQFVTEKDLPEIQRQSPDPYWRPRPKNLAEYWQQIRDYADIPRRYGNIKE
                     "
     gene            complement(3171..3509)
                     /locus_tag="PKZ53_03780"
     CDS             complement(3171..3509)
                     /locus_tag="PKZ53_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNJ39585.1"
                     /translation="MILWVDAQFSPAIATWMQTTFGIEAYALRDIGLRDATDLEIFSQ
                     AKAVGAVILTKDRDFIELLDRLEHPPKVIWITCGNTSNQRLKEILGSQFLKALALLEG
                     GETLVEITGM"
     gene            complement(3506..3718)
                     /locus_tag="PKZ53_03785"
     CDS             complement(3506..3718)
                     /locus_tag="PKZ53_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNJ39586.1"
                     /translation="MERITFNPDQCGGRPCIRGMRIRVTDILDLFAAGLSPEQILEEM
                     PDLEMADLQAALSYAARKLDHPVLAA"
     gene            complement(3850..>4600)
                     /locus_tag="PKZ53_03790"
     CDS             complement(3850..>4600)
                     /locus_tag="PKZ53_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonatase-like hydrolase"
                     /protein_id="HNJ39587.1"
                     /translation="LLQPDFFSPTSSARLLQPNFFSPNPMTDIRLVIFDLAGTTVHDT
                     GQVVAAFTTALAEHDISVTPDELSQVRGSSKRNAIRQFISQGPNQTEQADRVYAAFCQ
                     HLTQRYTTEGVTPIEGAAEVFKWLKSLGIKIALNTGFDRDITTLLVSALGWHTGIVDA
                     IVCGDEVRQGRPAPYLIFHAMEATGITSVHHVAAVGDTILDLQAGLHAGVRWNIGVLS
                     GAHSRQMLELEPHTHLLQSIREVPTLWSAAA"
BASE COUNT         1123 a         1092 c         1208 g         1177 t
ORIGIN      
        1 ctcatccttc atccctcatc ccttccgaaa accctgaacc ctgaaccctg aacccgttat
       61 cccaaaaact cctgaacatc cgcaagccac tgttccggtc gttcccaatg cagtgcatgg
      121 ccagtctccg gatatatttt cacttttgaa ctggtgattc cagtttcaag tgcctgctgg
      181 gcaggtgccg ggaagattgt gtcgtggtca cctcgcaaaa tcagagttgg tgattgaatc
      241 cggctcaatg ctgcggtgga atcaaattca agcaggctgg tgatgacttt atgccagacg
      301 ttggccgaga gtttctgact ttcagtgatg gcacgttcaa gaaattgatc cgggatggga
      361 aaaaagatgg tgctgacctg gaattcacgg ataaactcaa ctggtactgg atcatccagc
      421 gtcatcaccg cctgatgtaa ctccgcaatt tccgggcagc ggagattggt tgcggaaccg
      481 attaaaacca gccgtgccac ccgatccggg gcggaaaggg caacctgttg cgctaccaga
      541 ctgcccattg aatgccccac caccgttgct gaggaaaggt taagtgcgtc cataaacgcc
      601 acaacatcag ccgcaaaatc gggaacactg tagcccgttt ccggctggtc agaatctccg
      661 tgcccgcgct ggtcgagggc aaaaacgtgg gcggtttggg ccagcccatc aatcacctgg
      721 ctatatgaaa accacgaatc tgagtagccg tggagcaaaa tcactggcgg gttggctggg
      781 ttcccctgtt cggcatagtg caggcggact ccggttgaaa gctgaatatc ggcaaaacgg
      841 aatggaatta tcgaatcaga catggaaaaa gtctcctaaa aatcaacttg tgaataattg
      901 tacaagcgac tgaatatatt gaatttaaat gaggtactga tatatttgcc tggttagggc
      961 atttggggta ggacagttat cttacggatg aactgaaatt ctgacaagtt gaaaaagtgg
     1021 agtgcggcgg agtgcggtgg cttgacaccg ctttggtaaa aatgaacttt cagaaatgat
     1081 cactgcatgt agaatgtgtg cttcaaaaac aacgatgctc atcttaaaac tcagatcaat
     1141 caaaaattat gagtcaattt gccgccggca gctcacctga ccgttcttca aaattcgcca
     1201 aaatttctgt cattatcgcc gttgtaaacc tgggcatgat ggtgctgttg ttacttggag
     1261 tcgtgggaag tgaaaccttg cgcgatgtct tcaccgatga acgctggagc aagatgctgc
     1321 gcacaaccag tgatgtatgc tttatctcac tgatttatta tgcgctttca attggtccgc
     1381 ttgggttttt agttggaata tcgctgtcaa tcatcggaac atttcgaacc gggctcaaaa
     1441 aaggaatggg gatctttggc ctcgtttgta attttgtttt gtttgtcgga gtggtcattc
     1501 tcgatattgt gatcgggctg gcggcgattg ggttatccgc gttggctccc cggtgaaatc
     1561 cagcaatttc agattggtgt ttctgtcttt gaaggaacta aggccacctg ggaaaggcca
     1621 tagaggcacg gctaagaaaa agcacttctc cggctatcag tgcgcctgca gtcctgagat
     1681 tggtcctgga aagtagcctc catctggaaa aagaaataaa gagcgggttg acgttcccat
     1741 ttgccctgac cagaaactgg caccgactgc attcattttt cacagggttg aaacaatcgg
     1801 aaagatgaca aatttcgcat cgttgggcgt gagagacagt tttgattttc ataccttata
     1861 ttctctgaca gtttgtcgcc tctggagcga atacctccga caacgaagtg ctccaaacca
     1921 aagcggcgtc aagtcgccgc actccaaagt tttggttact ctttgatatt cccgtagcgc
     1981 cgggggatgt cggcataatc gcgaatttgt tgccagtact ccgccagatt tttggggcgg
     2041 ggccgccagt atgggtcggg agactgacgc tggatttcag gcaggtcctt ttcggtcaca
     2101 aattgctcat ccagcgtgca ggcttcgtat tcggaaaacg gcttttcata gatataccca
     2161 tccgccagtt gactgaggtg aaagccgtcg taacctgttg aataaaaact ggtatctgcg
     2221 aggtttccaa atggagttcc attcaagtca aagccaacgc gtttgtctga aaacgtcgcc
     2281 atgacctgat caatcgcgcc acgggccggg tacactgact ccatcgcacc gtttaatttg
     2341 ctctcgaaaa tctggtggag caggatcgta aagactttct tcggtgactg tttgtacaac
     2401 aaattgccga gcagtcggtt ttcaaaactg taaaactgcc gggcattgta atcaaaaacc
     2461 ggcatctgat agcgggtgaa ggcgtgcggc gtcccggtca ggacaaggat tttctctttt
     2521 ttctccagga tttcgcgttt gatcaggtcg gctcgatacc attccgttcc gcctttgtga
     2581 aacactttct tggtattttc cggagtccgt accaatgtgg cttcgctcca atcataacaa
     2641 tagctcaggt tgagaatgcg aaatttgcgc gctccagccg gcaatgtccg gttgagttcc
     2701 caggcggccc gatagacgtc gagatattcc ttgaaagccc atccgacatt gtaatgaaac
     2761 atcaaccggc gggcctgcgc ttcgtcatat ttttcagccg tgacgagttt gtcgagcgcc
     2821 gcctgatctt cgctcgcccc aaattccatc ccgaagttgt acaccccagc ccgatagagc
     2881 agcgaaatca ggttgtgcgc gaggtgcagg ttgtgtttga tgcggtggtc ctcggcgagc
     2941 aggatgatgt cgtggtcttt aaatttctcg atgatgtact gttcgggcgt tcgccagtgt
     3001 tgtttcaaaa aggctgccag ttgggtaact tctttttcag aaagtggttg cggcccatca
     3061 gccaataagg caatcggctg aatcaatacc cagaccaata gaaaaaatgg aagtaggcgg
     3121 gtgaagtatg gcatagagta cagcggggag agggcaaaaa agaatcttgg tcacattccc
     3181 gtaatttcaa ccaaagtttc cccaccttcc aacaaggcaa gggcttttag aaactggctt
     3241 cccaatattt cttttaaccg ttggtttgat gtgttgccgc aggtaatcca aatgactttt
     3301 ggaggatgct caagtcggtc gagtaattca ataaaatcgc ggtctttggt cagaatgact
     3361 gctccaactg ctttcgcctg actgaagatt tccaggtcgg ttgcatcccg taaaccaatg
     3421 tcacgcaaag cataagcttc tatgccaaac gtggtttgca tccaggtggc aatcgctggt
     3481 gaaaattgtg cgtctaccca aaggatcatg ctgccagtac cgggtgatcg agtttacgtg
     3541 ctgcatagga cagtgctgct tgtaaatcag ccatttcaag atcaggcatt tcttccagaa
     3601 tttgctctgg ggataaccct gctgcaaaga ggtcaagaat gtccgtcacc cggatgcgca
     3661 tgcctcgaat acagggccgc ccaccgcact ggtccgggtt aaatgtgatt cgttccattg
     3721 gagagtgctt ccttattaaa tggatgattt atgtgtttgt gaaggcactc taccagaagt
     3781 ctcatgaagt tgaaaactcc gacgtttgga gtgcggcggc ttgacaccgc tttggtatga
     3841 aaagcggcgt caagccgccg cactccacag ggttggtact tcccgaatgg attgtaacag
     3901 atgcgtgtgt ggctccagtt cgagcatttg ccgggaatgt gcaccagaaa gaacaccgat
     3961 gttccagcgc acaccggcgt gaagcccagc ttgtagatcc agaatggtgt ctccaacggc
     4021 agctacgtga tggactgacg taatgccggt ggcttccatc gcgtggaaaa tcaggtacgg
     4081 cgccggacgc ccctgacgga cttcgtcgcc gcacacgatt gcatccacga tgcctgtatg
     4141 ccagccaagc gccgaaacga gcaacgttgt gatgtcgcga tcaaagccag tgttgagggc
     4201 aatcttgatt cctagacttt tcaaccactt gaaaacctct gcggcgcctt caattggtgt
     4261 cacgccttcg gttgtgtagc gttgggtcag gtgttggcaa aaggcagcat acacccggtc
     4321 tgcctgttca gtttggtttg gcccttgaga gataaactgc cgtatggcat tccgttttga
     4381 tgaccctcgc acctggctca actcatcggg agtgacgctg atatcgtgtt cggcaagggc
     4441 tgtagtgaaa gccgctacga cctgtcccgt gtcgtgaaca gttgttccgg caagatcgaa
     4501 aattaccagt cgaatgtcag tcatagggtt ggggctgaag aagttgggct gaagaagtcg
     4561 ggctgaagaa gttgggctga agaagtcggg ctgaagaagt
//
LOCUS       DAOQDO010000167         4591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12570_length_4591_cov_3.199295, whole genome shotgun sequence.
ACCESSION   DAOQDO010000167 DAOQDO010000000
VERSION     DAOQDO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12570_length_4591_cov_3.199295"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKZ53_03795"
     CDS             complement(<1..228)
                     /locus_tag="PKZ53_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39588.1"
                     /translation="MQQSVYFFLAGLVMCSLFSPPLLATLLHPIKPTQRESVLLPSLQ
                     AESLVVNQRNPVVNEGRQLTLTVSTSNGQPVP"
     gene            1247..1645
                     /locus_tag="PKZ53_03800"
     CDS             1247..1645
                     /locus_tag="PKZ53_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39589.1"
                     /translation="MKSQTKKTPRRFHPSNTNRPKTKPNRPFSNEGPSMSSGMESPAP
                     TSVSFVDNLEGYLADQMRELIRARQEQEQALRDIRSLGEQLALGQEAPRLREQYEQAV
                     SSWQTSRRNGVNARQRLLVAMTERAVPDRK"
     gene            complement(1676..1909)
                     /locus_tag="PKZ53_03805"
     CDS             complement(1676..1909)
                     /locus_tag="PKZ53_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39590.1"
                     /translation="MNNGSNETESTDMILLAESDNYDVFTDQEADLIHVDFYGQGITL
                     HLTRADFLEMNQVLETALARLKTLVDDQEPTQL"
     gene            2096..2467
                     /locus_tag="PKZ53_03810"
     CDS             2096..2467
                     /locus_tag="PKZ53_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39591.1"
                     /translation="MDPVLADDIRALSLALGVEDDSFLCHPLNSQDPQLPEQIIDGLT
                     LAFVTFCYHRHPRGENVYELMDQLDAFDPDSVEAEALTERAEEAAALQIPFIVNMNRL
                     LEDYFQIRRRLEDMIAARDLK"
     gene            complement(2551..3414)
                     /locus_tag="PKZ53_03815"
     CDS             complement(2551..3414)
                     /locus_tag="PKZ53_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39592.1"
                     /translation="MKQIVTSLMVALCCIHSACTAAGGVSPQTSRQISEPLPSTSFSQ
                     LLESQRVHYKSESPAQRKIIAAYGAIWAARGNLVLPDRVVFANQTEVSLFQQKMRVGR
                     AQIGKTTIELQEAALVALLKARQDCLAKGCSLTPRGGASAARRDYVTTIHLWKSRVNP
                     GLNFHVKQKRITAQEAARIQNLSIPDQIEEILKLEAQGMYFSKSLDKSILYSVAPPGA
                     SQHLALLAIDIEEYENAQVRAILANHGWFQTVISDLPHFTYLGLSEAELTARGLKKEV
                     NGGRVYWVPNW"
     gene            complement(3420..>4591)
                     /locus_tag="PKZ53_03820"
     CDS             complement(3420..>4591)
                     /locus_tag="PKZ53_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39593.1"
                     /translation="GGSTNYSVTLTRTNFTGPVDLTVRGIGGPSPRVGLDAAFTPDNT
                     TGNSSTLNIRTNIGGTTLAGTYTFRVTGTASGATVVDSNVFSLVVSSATPPSVVIGVE
                     PTSQRVIAGDIAQYRLTVTKTNYSGSVLLSLVGNVPPNTTVSFNPPQFDTSTTMIFDT
                     SSNTTHGTYVMQIVGEAQGVTIQPVTFSLQVDAPSPPTITLSMNPTSRTIMSGETANY
                     QVTVTTTNFSGSVNLSVTGNIPPATSFSFNPPQFTSTTTLIFTTSGNTPSGTYNMQLV
                     GTSTPSVSITPVNFTLIIQNVGLRKTAQLIQTSPGTGQLGSKEIRVNALPISSELAQP
                     ATFPISETISSKMVQELPDVRGLVKLKLCDLGSQIRRPAKNSGPDQPNILTVLKF"
BASE COUNT         1141 a         1184 c         1069 g         1197 t
ORIGIN      
        1 cggtaccggc tggccgtttg aggttgaaac agtcaacgtc aactgccggc cttcattgac
       61 aaccggattc cgctgattga ccaccaatga ttcagcttga agagagggta aaagaacgct
      121 ttcccgttgc gtgggcttga ttggatgcag cagagttgcc agaaggggag gagaaaataa
      181 ggaacacatc acgagtccgg ccagaaaaaa atacactgat tgctgcatca gaacacgact
      241 cgaaaaaaaa atgggttttc ccataccgca taccataagc catttacttt gaataattta
      301 aaaattgatc aaatattcaa acgataggtc gagagcaaaa atccagaaga tttcgtgaaa
      361 attcaccctg agtatggaat cgaattcatt ctggatcaag aattttcagg caagatcagt
      421 ttccggatac caaaatggct tccgatgggc ggcacccatc ctggatctgg tgagcgcggc
      481 ttgacaagct tttcccaact gactatggtt gatggcatct gcaatctctg cagcaaatcc
      541 aacaaaacaa gcaccttttc gcaatcttcc actttttgtg aatgctcgtt gtttactttc
      601 tgagtaaata gcgtctgtgc ttggaaccgc tggttgacgc ttttttcagc ttctcactgt
      661 ctgattcact catcagcacc gttttaccat caacaccaca ttgaaaaaat ggctgctttg
      721 gctgaaaaaa gcaccggttg tgcgtcttca agcccctcat taaggtaact gctgtaagct
      781 gcccctcttc ccccgtcatt taccttctat gatcaggatt gctgctcaac tcgaaatctc
      841 gcttaaaaga gatcaatctg gagaggattt gaacccgtct tcgacgcatt gggtcatcat
      901 caaccgtacc agtcaccctt aattccattc agtattcatt gagagttaaa gccgggatca
      961 ccctgggttg ctttctggcg tgatggctca acgagcccat ctgattttgc cagactgagt
     1021 accgactcaa tcctgaccgt ggcagattgt tcaccattac ccactttgca ccgatttacc
     1081 ttctcacgtc gctacagaca ccagtccaac tgttcctggt tgactgccta caagctggtg
     1141 gcaactctgt ttttatctcg ctgacataca gcgcgagcca gaacaactgc cgcgagcata
     1201 acttatttca atctgccagt ttcgactttg aggtcaaaga gaacttatga aatcacaaac
     1261 caaaaaaaca cctcgccgct ttcatccatc caatacaaac cgtcccaaaa ccaagccaaa
     1321 tcgccctttt tccaacgagg gcccgtcaat gagttccggg atggaaagtc cagcccccac
     1381 ctcagtgtca tttgttgata acctggaagg ctatctggca gatcaaatgc gtgaattaat
     1441 ccgcgcccgc caggagcaag agcaggcgct gcgtgacatt cgatcactcg gagaacaact
     1501 tgccctgggt caagaagcac cccgcttacg tgagcaatac gaacaggctg tttcatcctg
     1561 gcaaacctcc cgccgaaatg gtgtcaatgc tcgccaacgg ctcctggttg ccatgactga
     1621 acgcgcggtg cctgaccgaa agtaaacctg gcaagatctc aggcacagac ccggctcaga
     1681 gttgagtggg ttcctgatca tcaaccagtg ttttgagtcg ggcgagtgcg gtttctaaaa
     1741 cctgattcat ctccagaaaa tcagctcgcg tcaaatgcaa cgtgattccc tgcccataaa
     1801 aatcgacatg gattaaatcg gcttcctggt cagtaaacac atcataattg tctgactcgg
     1861 ccagcaaaat catgtcagtg ctttctgttt catttgaacc gttattcata acgttctctc
     1921 tttcgtttgt ggttggttaa ctggcttaaa tcaaggaaga aacatcatct ttggccagcg
     1981 taccggaagg tttgacacgg atgtctaata tccatctgga catagtcgta cctgcctatg
     2041 gtaaactgcc gaccgttctg ctgttaagtt taatcttttt ctttttttca gacttatgga
     2101 tcctgtatta gctgacgata tccgagcact ttccctggcc cttggcgttg aagatgattc
     2161 ctttctctgt catccactca atagccagga cccgcaactc cctgaacaaa tcattgatgg
     2221 gcttactctc gcgtttgtga ccttttgcta ccaccgtcac ccgcgcggtg agaatgtgta
     2281 tgaattgatg gaccaactcg acgcatttga tcctgattcg gtcgaggcgg aggccttgac
     2341 ggaacgagct gaagaagcgg cagcacttca aattccgttt attgtgaata tgaaccgatt
     2401 gctggaagat tacttccaga ttcgccgccg acttgaagat atgatcgctg cccgagacct
     2461 caaataaccc atcattgtgc cgtctggttg ctcctgcctt tgcccccagg tgcttgtttg
     2521 ccatccactt gctctcactc ttcaggggga ctaccagttg gggacccagt agacccgccc
     2581 cccgtttact tccttcttca gcccacgggc cgtcaactct gcctctgaca gtcctaaata
     2641 cgtaaaatgc gggagatcag aaatcaccgt ctgaaaccat ccgtgatttg ccaggatggc
     2701 tcgaacctgt gcgttctcat attcttcaat atcaattgcc agcaaagcca gatgctgtga
     2761 ggcaccaggg ggagccaccg agtacaaaat agatttatcc aggcttttgc tgaagtacat
     2821 gccctgcgct tcaagtttta aaatctcttc aatctgatct ggaattgata aattctgaat
     2881 ccgtgccgct tcctgcgccg tgattctttt ttgtttgacg tgaaaattca gtcccggatt
     2941 gacccggctc ttccataaat gaatggttgt cacataatcg cgcctggcgg ctgaagcacc
     3001 gcctcgggga gtcagactgc accctttggc cagacagtcc tgccgggctt tgagcaaggc
     3061 caccagggcc gcttcctgaa gttcaatcgt tgttttgcca atttgagccc gcccaacccg
     3121 cattttttgt tgaaagaggc tcacttcggt ctgatttgca aaaacgaccc ggtcggggag
     3181 caccagattc cccctggctg cccagatcgc cccatacgcc gcgatgattt tgcgttgagc
     3241 cggactctct gatttatagt gaacccgttg agattcaagg agttgagaaa atgaggtgga
     3301 aggcagcggt tcagaaattt ggcggctggt ttggggggaa acccctccag ccgccgtgca
     3361 ggcactgtga atacaacaca gtgcaaccat caaacttgtc acgatttgct tcatgggcat
     3421 tagaatttca agactgtcaa tatattgggt tgatctggac cagagttttt cgctggcctt
     3481 ctgatctggg accccaggtc acacagtttc agctttacca ggcccctgac atccggtaat
     3541 tcctgtacca tttttgaact gattgtctca ctgattggaa aagtcgcggg ctgggccaac
     3601 tcactggaaa tcggaagtgc gttaaccctt atctcttttg agcccaactg gcctgtacct
     3661 ggtgaagttt gaatcagttg ggcggttttc cgaagaccaa cattctggat aatcagagtg
     3721 aagttgactg gtgtgattga taccgacgga gtgcttgtcc caacaagctg catattatag
     3781 gtccctgacg gagtatttcc gctggtagta aaaatcaggg tggtggtgct ggtaaattgc
     3841 ggcggattaa aagaaaagga ggttgccggc ggaatgtttc ctgttaccga gagattcacc
     3901 gacccgctga aattagtcgt cgtgaccgtg acctgataat ttgccgtctc gcccgacata
     3961 atggtccgac tggttgggtt cattgaaagc gtaatcgttg gcggagacgg cgcatcaacc
     4021 tgtaaactaa aggtgacagg ctggatcgtt accccctggg cttcgcccac aatctgcatg
     4081 acataggttc catgagtggt attagacgaa gtatcaaaaa tcatggttgt tgatgtgtca
     4141 aactgtggcg gattgaaact caccgttgta tttgggggta cattcccaac cagcgacaac
     4201 aaaactgacc cagaataatt cgtcttggtt acggtgagcc gatactgcgc aatatctccg
     4261 gcaatcaccc gctgactggt cggttcaact ccgatcacca ctgacggtgg agtcgcactt
     4321 gaaacaacca ggctaaacac atttgagtct accacggtcg cgccggaagc agtccctgta
     4381 acccgaaaag tataggtacc agccaaagtt gtgcctccga tattggtccg aatattgaga
     4441 gttgagctat tcccggtggt gttatctggt gtaaacgcag catccagccc cactcgtggc
     4501 gacggtccgc cgattcctct caccgttaaa tcaaccgggc cagtgaaatt ggttcgtgtc
     4561 aaagtcacgc tataattggt gcttccgccc g
//
LOCUS       DAOQDO010000168         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12596_length_4586_cov_3.125359, whole genome shotgun sequence.
ACCESSION   DAOQDO010000168 DAOQDO010000000
VERSION     DAOQDO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12596_length_4586_cov_3.125359"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1422)
                     /locus_tag="PKZ53_03825"
     CDS             complement(103..1422)
                     /locus_tag="PKZ53_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39594.1"
                     /translation="MNSLFDHWYLSLIPLAGYLLLTVISMKRISEVRTQLRGVIRTRK
                     DLELVKTAINQNMRLALVLILGGGGYLLLLVGLLASKILPASDSWHLTVFTVVMALSS
                     VVVHILEKPLKTLHVEAKDPRIAQTYQTWLRQWSQARWGLQEDIVETLPPPVNFGTQS
                     VHNESLSERTMDQQPHLQFSSNLPPPARKELALLPPRPIRRRAGRGWLIGFQQIFILP
                     HTFVGIGLIVFMVGSWIFLIFGITVPAQITELKTTRGSKNTTAYEVHFRYQHFGAEHH
                     ERTTVPKAVFDTLHQNDVTEVKVFPLPISKLTQLKYPGSFDSHVVKVMVSTAMALFWN
                     GIVSVFLWAFYVRPLINRWLVQNGWPASGQITGKSVKGGKTTTYYVEYQFALPKEGTK
                     QGKMEVTADAYSLVNQGDLVSILYSTKNPGWNVVYELAEYEVVTNPY"
     gene            1806..2369
                     /locus_tag="PKZ53_03830"
     CDS             1806..2369
                     /locus_tag="PKZ53_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ39595.1"
                     /translation="MSSEHSASQALTDQTQLAHFESHVFPHTAVLLRSAIRMWGNPTE
                     AEDIVQDTLLRAWKYWDRFEPGTNCRAWLFRIMINVINRRREGLEAGSAHVAAHEPEI
                     TNVLRFEPRIELDEHGTLHALEKLPSDYRDVLMLVLVEELSYKEAAVMLNIPMGTVMS
                     RLHRARQMMKKLLRPGSSTSHEAATSH"
     gene            2408..3325
                     /locus_tag="PKZ53_03835"
     CDS             2408..3325
                     /locus_tag="PKZ53_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ39596.1"
                     /translation="MNQMQCEQVNDLLSAYVDNEVDAVTAMKIAHHLERCQACRHEYQ
                     LTKQVSDVLKTAVRESPDLEPALEVRAFQLNNRVHDLLTAQLGQPKPPISGEIPQLDA
                     EILPPPEAVPPSPVPFKPRWTTGRIVKTILTIAASLVVMVGLGYGGFLVWLENSGTFS
                     VAIARDYRVCNGNPGLVAWYRDGKPVPNRTPIDAVGRKLNQMGYTPDGLALCKIGGVP
                     FVHTFYLRDHQPLSVYFGPKQAVAFLKSEKGDARPGQMYESQSDTIAMSAFVSPSEEN
                     VWVVAGELSRTEINAITHDLMSETLAKRE"
     gene            3933..>4586
                     /locus_tag="PKZ53_03840"
     CDS             3933..>4586
                     /locus_tag="PKZ53_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ39597.1"
                     /translation="MTNPVSVDEPSTPKRIPLNPDLKSPRAQEFEDKLLARIVGQERA
                     VRRLVNLYQIYLAGLSNPGRPIGTMLFLGPTGSGKTRVVEAAAEVLFTDSNAVIKIDC
                     AEFQHSHEVAKLIGSPPGYLGHRETAPMLTQENLDRYHTERDQLTFVLFDEIEKASDA
                     LWQLLLGILDKATLTLGDNRRVDFSRTMIILTSNLGAREMSELITGSIGFAPPRMGRD
                     "
BASE COUNT         1219 a         1190 c         1112 g         1065 t
ORIGIN      
        1 ccaagaacca agaaccaaga accaagaacc aagaaccaag aaccaagaac caaacccttc
       61 ttcattcttc attcttcatt cttcattctt cattcttcat tctcaataag gattggtgac
      121 gacttcatac tcagcgagtt catacacaac attccaacct gggtttttgg ttgagtacaa
      181 aattgaaacc aaatccccct ggttgaccag cgaataggca tcggcggtca cttccatttt
      241 cccctgtttt gttccttctt tgggtaaggc aaactggtat tcaacataat aggtggttgt
      301 tttcccgccc ttaaccgact taccagtaat ctgacccgat gccggccagc cattttgaac
      361 cagccaccgg ttgatcaggg gtcgcacgta gaatgcccac agaaacaccg aaacaatccc
      421 attccaaaac agagccattg cggttgaaac catcactttc accacgtgcg aatcaaagga
      481 acctgggtat ttcaactggg tgagttttga aatcggaagt ggaaaaactt tgacttcagt
      541 cacgtcattt tgatgcaagg tgtcaaaaac agctttggga accgtggttc gctcgtgatg
      601 ctcggcgcca aagtgctgat agcgaaagtg aacctcgtag gcggttgtgt tttttgatcc
      661 acgcgtggtc ttcaactctg taatctgtgc cggaacggtg attccaaaaa tcaaaaatat
      721 ccagcttccg accataaaca caattaaccc aatcccaaca aaagtgtgcg gcaaaatgaa
      781 gatctgttga aacccaatca gccatcctcg tccggcacgg cgacgaatgg ggcgcggtgg
      841 aagtaaggcc agctctttgc gggcaggagg tgggagattg gatgaaaact ggagatgggg
      901 ctgctgatcc atagttcgct ccgagagtga ttcattatga acagactgag ttccaaagtt
      961 cacaggtggc ggaagcgttt caactatatc ttcctgtaat ccccaccgcg cctgggacca
     1021 ctggcgaagc caggtttggt aggtttgggc aattcgtggg tcttttgctt caacgtgcag
     1081 cgttttgagc ggtttttcga ggatatgaac cacaacagag gacaaagcca taaccactgt
     1141 gaacacagtc aaatgccagc tatcgcttgc cggcaaaatt tttgacgcca gcaatcccac
     1201 cagaagcaat aaatatccgc caccgcccag gatcaagacc agggccagcc tcatgttctg
     1261 attgatcgcg gttttgacca gttccagatc cttgcgagtc cgaatcacgc ctctaagttg
     1321 agtgcgaact tcagagatac gcttcattga gataaccgtc agcaacagat acccggcaag
     1381 tggaatcagg ctcagatacc agtgatcaaa cagggagttc attgtggtcc tcgattgcgg
     1441 gtcgggttcc gggtttgcgg aagggatgag ggatgaagga tgagggatga aaaaatcacc
     1501 atgtcacctt gtcagtcggt cagcgtccag gttccgggtt ttcgaaacca ggcaccaggc
     1561 accaggcacc aggcaccagg caccaagcac caagcaccaa gcaccaggta ccaagcacca
     1621 ggcaccaggt accattccat catcaatcaa acaattcttt tgcttgaaat catactctca
     1681 aactttttcc agaggtgaaa taaatcacaa cctcactgga tttcgccggc ataacccaaa
     1741 aacaaatccc ctcgacaaac ttaggatgat tcacaatgcc gctcgaaatc ccagaggaga
     1801 acagcatgag tagcgaacat tctgcttccc aggcgctcac cgatcagaca cagcttgctc
     1861 actttgaaag ccacgttttt ccgcatacag cagtgctgct tcggagtgca atccgcatgt
     1921 ggggcaatcc cactgaggcg gaagatattg tgcaggacac gctgcttcgc gcctggaaat
     1981 attgggatcg gttcgaacca ggcaccaact gtcgggcctg gttgtttcga atcatgatca
     2041 atgtcatcaa ccgtcgccgt gaaggactcg aagccgggtc agcccacgtc gccgcccacg
     2101 aaccggaaat caccaacgtg ctgcggtttg agccgcgcat tgaacttgac gaacacggca
     2161 cgctccatgc cctggaaaaa ttgccgtctg attaccgtga cgtgttgatg ctggtcctgg
     2221 tcgaagaact ttcctacaag gaagccgccg tgatgctcaa catcccaatg ggaacggtga
     2281 tgtcacgtct gcaccgcgcc cgccagatga tgaaaaaact ccttcgtcca ggaagtagta
     2341 ccagccatga agcagccact tctcattgag ttcgcaaaga gctgtcggga gaaaaaaaga
     2401 ggtcagtatg aatcagatgc agtgtgaaca ggtaaatgat cttttgtcag cctatgttga
     2461 caacgaagtt gacgccgtga cagccatgaa gatcgcccac cacctggaac ggtgtcaggc
     2521 gtgccggcac gagtatcaat taaccaaaca agtctcagat gtcctgaaaa cggcggtcag
     2581 agagtcaccg gacctggaac cagccctcga agtacgggca tttcaactca acaatcgtgt
     2641 ccacgacctg ctgactgccc agcttggcca acccaaacct ccaatctcag gtgaaattcc
     2701 ccagcttgat gcagaaatcc taccgccacc agaggcagta ccgcccagcc ctgtcccctt
     2761 caaacccaga tggacaactg gacggatagt gaaaaccatc ctcaccattg ccgcttcgct
     2821 ggtggtgatg gttgggttag gctatggcgg ctttctggtt tggcttgaaa atagtgggac
     2881 gttttcagtc gccattgccc gtgactaccg ggtctgcaat ggcaatccgg gactggtggc
     2941 gtggtatcgg gatggaaaac cggttcccaa ccggacccca attgatgcgg tgggcagaaa
     3001 actgaaccag atgggctata ctccagatgg gctggccttg tgcaaaatcg gtggtgtgcc
     3061 gtttgtccac acgttttacc tgcgcgatca tcagccgctc tcagtttact ttggacccaa
     3121 acaagctgtg gcatttctca aatctgaaaa aggggatgcc agacctggtc agatgtacga
     3181 atcccaaagt gacactatcg ccatgtcagc ttttgtttcc ccaagtgaag aaaatgtctg
     3241 ggttgtggcg ggcgaactct cacgaaccga aatcaatgcc atcacccacg acctgatgtc
     3301 ggagacactc gccaaacgag aatgaagaat tgagaatgaa gaattgagaa tgaagaatga
     3361 agaatagaca ttatcggaga taaaaccggg ccatcacaac aaggacggcg acaagtcagt
     3421 accgcccgcg tgagcgggtg ggttgccact gtgagaaagc tccactttgg gaaaatggtt
     3481 aaccccgata atgtttaatg agtttggtac ctggtatttt gacctaccat tgaagaacca
     3541 agaaccagga accaagagcc agggatttct tcattcttca ttctcaattc ttcattcttc
     3601 attctcaatt cttcactctt cattcttcat tcttcaccag ggttgctttt cgtcaggaaa
     3661 actggaaata tttccggcgc tgctatacaa attcaaagct tttattcatt tcgggatatc
     3721 catcccataa gctgaagttt tcgggaggcg agatgtttga cgattgaacc cggcgtccgg
     3781 cattgtttcc agtgctgacg ccaagccaac gcccacttcc tgcaaaacat catttgaaac
     3841 gactgagtga gtctgacaca ttgtgtggta agtctcactc attgttgttt ttaggcattc
     3901 gaatttcgag acatcaggga gcgcgaaatc tcatgacaaa tccagtttct gttgacgaac
     3961 cttctacacc gaagcggata ccacttaatc cggacctcaa aagccctcgg gcccaagaat
     4021 ttgaagacaa gctgctggcc cggatcgtgg gtcaggaacg ggctgtccga cgacttgtca
     4081 acctgtatca gatttatctg gcagggcttt cgaatcctgg gcgtccgatt gggaccatgt
     4141 tattcctcgg accaaccggc tcggggaaaa cacgcgtcgt cgaagccgcc gctgaagttt
     4201 tattcactga ttccaacgcc gttatcaaaa ttgactgcgc cgaatttcag cacagccatg
     4261 aagtcgccaa attgattggt tctccgccag ggtatctggg acaccgcgaa acggccccaa
     4321 tgttgaccca ggagaatctg gaccgatacc acaccgagcg tgaccaatta acgtttgttt
     4381 tatttgatga aatcgaaaaa gcttcagacg cgctgtggca attattgctg gggattttgg
     4441 ataaggccac attaacgctt ggcgataatc gccgggtgga cttctcgcgc accatgatca
     4501 ttttgaccag caacctgggt gcccgcgaga tgtcggaatt gattaccggc agcattggat
     4561 ttgccccacc ccgcatggga cgtgac
//
LOCUS       DAOQDO010000169         4583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12611_length_4583_cov_2.886484, whole genome shotgun sequence.
ACCESSION   DAOQDO010000169 DAOQDO010000000
VERSION     DAOQDO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12611_length_4583_cov_2.886484"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKZ53_03845"
     CDS             <1..393
                     /locus_tag="PKZ53_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ39598.1"
                     /translation="AGLDPAERNRFLDLLSEIGENVIVILSTHIVEDVKELCTQMAII
                     NKGRVLLSGNPVEAIESVHGKIWKKFIHKSELETFQREFDIISTRLVAGKTEIHVYAD
                     SQPDPSFHPTETDLEDVYFHTLKNGATV"
     gene            502..4140
                     /locus_tag="PKZ53_03850"
     CDS             502..4140
                     /locus_tag="PKZ53_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNJ39599.1"
                     /translation="MFAEIFRFEMNYRFKRISTYMYFGLWFLMGFLYTSTEGLSIGVG
                     GKVLKNGPYGISMMTAVLGAFGVLITSAIFGTAIHRDFEQNTYSTLFTTPLNKRSYLG
                     GRFLASYLVMLLVYAGIPLGMLIGGLMPWADKTKLMPVHLANYFQPFLVLAVPSIFLT
                     GALFFLVGALSRSLLAVYLQGVVFFALYLITLPLIREIGNDTLPGLADPLGIVAINVI
                     TKYWTVAERNSQLLPMTGVLLYNRLIWMGVGAVALVLVMRFFTFSSEKLSFFGSGKKK
                     ANLAEPAVPKPAHLHLPVVQQLFTGSTRWLQYLTLTRLNFLSVVKEIPFMAIVFIGMF
                     ILMLNGSQAGKFYGTQVFPVTYIIIEVIQGGFGLFFIILVTMYAGELVWKERGVKFDQ
                     IHDALPLPGFLTYLSQLTALTLVYSVLLLALTLVGMGIQVWHGYFKFELALYLKEAFL
                     IQLPSLIQVTAVALLAQTVLKNKFLGHAVVILFFMSLGFIGEFGLEHKLYRYSSNASY
                     VYSDMNGFGHFAKPMLWFTVYWTACAGLLAVLTVLLSVKGVDTSWKARVQNARQNLRF
                     PLTAILGVLAVTFVGSGVYIFYNTNILNKYRTSDDTKAAVKEYEKLYKKYEKIPQPRI
                     TAVNLNVDLFPETREFRAKGSYVLENKTKTDITAIHITDQSDTIKNLNFSRQVKETLK
                     DVDHSYRIYELPTPLKPGEHMELAFEVEEKNPGFRNSGENNSIVENGTFFNSSYFPKI
                     GYQPDAEMRDEDERKKLGLPPRPGMAPPDNLDARMNTYIANDADWTTFNATVSTSPDQ
                     IAIAPGYLQREWLENGRRYFEYKMEAKVLHFFAVLSGRYQVKRDRWNDVNIEVYYHPA
                     HAYNLDRMIQSVKKSLDYYTVNFGPYQHKQFRIIEFPRYASFAQSFPNTIPYSESIGF
                     IARVVKDDDIDYPFYVTAHELAHQWWAHQVIGGNVQGSTLLAETLSQYSALMVMEKEY
                     GKEKMRKFLKYELDRYLRGRTAERKNEPALAYVEAESYVYYQKGSLVMYALKDYIGED
                     KVNAALKKFIKECGFQNPPFTNSLEFLKYIREVTPPEMQYLITDLFETITLFENRAIE
                     TSYSKTPDGKYLVKLKVDAKKLRADGSGDEKEIAINDLIDIGVFSGDKKKEKVLHLQK
                     YRIDKPQMEFEITVDEEPARAGIDPYNKLIDRNSDDNVIEISQKSQSGKPVGIKIQSG
                     DQPNQMRD"
     gene            complement(4280..>4583)
                     /locus_tag="PKZ53_03855"
     CDS             complement(4280..>4583)
                     /locus_tag="PKZ53_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39600.1"
                     /translation="ISFLEDIVSRSNYEIIVINPEKHRLGMKESAAQLKQRTKRWVYT
                     SLGPSEALRFSYGMSQAFDFSRPGVYTIIVKRAGDSGEVVSNTTKLVISPASPTKL"
BASE COUNT         1167 a         1104 c         1117 g         1195 t
ORIGIN      
        1 gctggcctcg atccagccga acgaaaccgg tttctggact tgctgagtga aatcggtgaa
       61 aacgtgattg tgatcctctc aacccacatt gtcgaagacg tcaaagaact ctgcacccag
      121 atggccatta tcaacaaagg ccgggtcctg ctttcgggaa atccagtcga ggcgattgaa
      181 tcagtccatg gaaaaatctg gaaaaaattc atccataaaa gcgagcttga aacttttcaa
      241 cgtgagtttg acatcatttc cacccgcctg gtcgccggga aaactgaaat ccatgtctat
      301 gctgacagcc agccggatcc ttcatttcac ccaactgaaa ctgatctgga agacgtgtat
      361 ttccacacgc tgaaaaacgg cgccacggtt tgaaaaacca gggttcagcg tgaaaggaca
      421 tccaggacga aagggacata aaatcgaaac ccctgaaccc tgaacccctg aacccttaat
      481 ggaaagattg tgcaggtgga tatgtttgct gaaatttttc gctttgaaat gaattaccgg
      541 tttaagcgca tctcgactta tatgtatttc gggctgtggt ttctgatggg gtttttgtat
      601 accagcaccg aagggctcag tattggtgtt ggtgggaaag ttctcaaaaa tggcccctat
      661 ggaatcagta tgatgacggc ggtgctgggt gcctttgggg tcctgattac ctcagccatt
      721 tttggcacgg ccattcatcg ggattttgaa caaaatacct attcaaccct gtttaccaca
      781 cccctcaaca agcgcagcta tttgggaggc cggtttctgg cgtcctatct ggtgatgttg
      841 ctggtgtatg ctggaattcc gcttggaatg ttgattggag gcttgatgcc ctgggcggat
      901 aaaaccaagc tcatgccggt gcatctcgcc aattattttc agccgttcct ggtgctggct
      961 gttccctcga ttttcctcac cggcgccctt ttctttctcg tcggcgctct ttcccgaagc
     1021 ctgctggcgg tgtatttgca aggtgtggtg ttttttgcgt tgtatttgat cacgctcccg
     1081 ctgattcggg aaatcggcaa cgacacgctg cccggtctgg cagacccgct tggcattgta
     1141 gccatcaatg tcattaccaa atattggacc gttgctgaac gcaacagcca gttactgccg
     1201 atgaccgggg ttttgctcta taaccggttg atttggatgg gcgttggtgc tgttgccctg
     1261 gttctggtta tgcggttttt tacattttct tccgaaaaac tttccttctt tggcagcggg
     1321 aagaaaaaag cgaaccttgc cgaaccggcg gttccaaaac cggcacatct gcacttaccc
     1381 gttgtccagc agttgtttac cgggtcaacc cgctggctgc agtacctgac cctgacccgg
     1441 ttgaattttt tgagtgtcgt caaagaaatc ccctttatgg cgattgtgtt catcggcatg
     1501 tttattctga tgctcaatgg ctcacaggca gggaagttct atggaacgca agtctttcca
     1561 gtgacttaca tcattatcga ggtcattcag ggcggatttg gcctgttctt tatcattctg
     1621 gtcacgatgt atgctggtga actggtttgg aaagagcgcg gggtcaaatt tgatcagatt
     1681 cacgatgctc tgccgctccc tggatttctg acctacctca gccagttgac agcgttaaca
     1741 ctggtgtatt cggtgttgct cctggcatta actctggttg gaatgggaat tcaggtctgg
     1801 catggctact ttaagtttga actggccctg taccttaaag aagcctttct gattcagctc
     1861 ccatcactga ttcaggtcac cgctgtggct ctgctggccc aaacggtact caagaacaag
     1921 tttctgggtc acgcggtggt gattttgttc tttatgtcgc ttgggtttat tggcgaattt
     1981 ggcctggagc acaaactcta tcgctatagc agcaatgcgt cttatgtcta ttcggatatg
     2041 aacgggtttg gtcattttgc caaaccgatg ctctggttta cggtgtactg gacggcctgt
     2101 gccgggctgc tggcggtgct cacggtattg ctctcggtca aaggggttga tacaagctgg
     2161 aaagcacggg ttcaaaatgc ccgccagaat ttgcgcttcc cgctgacggc aattctggga
     2221 gtgctggcgg tgacatttgt tgggagtggt gtatatattt tttataacac caatattttg
     2281 aataaatacc gtacttctga tgacaccaaa gcggcggtga aggaatatga aaaactctac
     2341 aaaaagtatg agaaaatccc tcaaccccgc atcaccgccg tcaacctcaa tgttgatctg
     2401 tttcccgaaa cccgcgagtt tcgagccaaa ggcagttatg tccttgaaaa caaaaccaag
     2461 acggacatta ctgccattca cattacggat cagtctgaca cgatcaagaa tttaaatttc
     2521 tcccgccagg tcaaagagac gctcaaagac gtagatcaca gctaccgaat ttatgagctc
     2581 cccacgccgc tcaaaccagg cgaacacatg gaactggcat ttgaggttga agagaaaaac
     2641 cccggctttc gcaacagcgg ggaaaacaat tcgattgttg aaaacggcac atttttcaat
     2701 agttcgtatt ttccaaaaat cggctatcag ccggatgcgg aaatgcgaga tgaggatgag
     2761 cgcaaaaaac tgggcctgcc gccacgacct ggaatggccc cccctgacaa ccttgacgcc
     2821 cggatgaaca cctatatcgc caatgatgct gattggacga cctttaacgc cacggtcagc
     2881 acttctcccg atcagattgc gattgctccg gggtatctgc aacgtgagtg gctcgaaaat
     2941 ggccggcggt attttgaata caaaatggag gccaaagttc tccacttttt tgctgtcctg
     3001 tctggccgct atcaggtcaa acgggatcgg tggaatgatg tcaacatcga ggtctactat
     3061 cacccggcgc atgcctataa cctggaccgg atgatccagt cggtcaaaaa gtcgctcgat
     3121 tactacaccg tcaatttcgg gccgtatcag cacaaacaat tccggattat tgaatttccg
     3181 cgctatgcct cgtttgctca gtcattccca aacacgattc catattctga aagcattggg
     3241 tttatcgccc gggtggtcaa agatgacgac attgattacc cgttttatgt gactgcccac
     3301 gaactggcgc accagtggtg ggcacatcag gtcatcggcg gcaatgtcca gggttcgacg
     3361 cttctggctg aaacgctgtc gcagtattcg gcgctgatgg tgatggaaaa ggaatatggg
     3421 aaggaaaaaa tgcggaagtt cctcaagtat gaactggacc gctatctacg cgggcgcaca
     3481 gcagaacgca aaaacgaacc agcactggcc tatgtcgaag ctgaaagtta tgtctactat
     3541 cagaaaggca gtctggtgat gtatgccctc aaggactaca ttggcgaaga caaggtcaat
     3601 gcggcgctga aaaaattcat caaggaatgt ggtttccaaa atccgccgtt taccaattcg
     3661 ctggagtttc tcaagtacat tcgcgaagtc acgccaccag aaatgcagta tttgattacc
     3721 gacctctttg aaaccattac cttatttgaa aatcgggcga ttgaaaccag ctacagcaaa
     3781 accccggatg gcaaatacct ggtcaagctc aaagttgacg ccaaaaaatt gcgggcagat
     3841 gggtcaggcg atgaaaagga aattgccatc aatgacttga ttgatatcgg cgtgttttca
     3901 ggggacaaga agaaagaaaa agttcttcat ctccagaaat accgaattga caaacctcag
     3961 atggaatttg aaatcactgt ggacgaagaa cccgcccgag ctggaattga cccgtacaac
     4021 aagttgattg accgcaattc agacgacaat gtgattgaaa tcagtcagaa gtcccagtcc
     4081 ggcaagccag tgggaatcaa aatccaatca ggcgaccagc cgaatcagat gagggattga
     4141 ccgtaactgg tttgtgcgcc aacgaagttt cggttttaag ctgcaagcaa atatcaactc
     4201 gtcgcccagg gtggagtctt caaaaccctg ggcgacgtgc ctgttgcccc gttcctgagt
     4261 tcaacccctg ggttcttggt tacaacttcg ttggactggc aggtgaaata accaattttg
     4321 ttgtatttga caccacctcg cctgaatcac cagcccgctt tactataatt gtgtaaacac
     4381 caggtcttga aaaatcgaaa gcctgactca ttccataaga aaagcgtaag gcttcagagg
     4441 gtcccagaga tgtataaacc caacgtttcg ttctctgctt taattgagca gcactctctt
     4501 tcatccccag cctgtgcttc tctggattga tgacaatgat ctcataatta cttctggata
     4561 ctatatcttc caaaaatgag att
//
LOCUS       DAOQDO010000170         4577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12630_length_4577_cov_2.915966, whole genome shotgun sequence.
ACCESSION   DAOQDO010000170 DAOQDO010000000
VERSION     DAOQDO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12630_length_4577_cov_2.915966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKZ53_03860"
     CDS             complement(<1..1070)
                     /locus_tag="PKZ53_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNJ39601.1"
                     /translation="MIDKLLTRIFGSANERYLKRIRPIVARINDLEPEIQQLTDDQLR
                     AQTVKFKERLDAGESLDDLLPEAFATVREASRRTTTMRHFDVQMIGGIVLHEGRIAEM
                     RTGEGKTLVATLPSYLNALTGKGVHVVTVNDYLARRDAEWMGRIHKFLGLSVGVIQSN
                     IDDFTRREAYGADITYGTNNEFGFDYLRDNMKFSLAQCVQREYNFAIVDEVDSILIDE
                     ARTPLIIAGASDDSTEKYYLANDIIPRLNGETDFLVDEKTHTATLTEDGIEHAEKLLG
                     CGNLYDPANREILHCVNQALRAHTLYKVDKDYIVKDGEVVIVDEHTGRMMTGRRWSDG
                     LHQAVEAKEGVKIEKETQTLATV"
     gene            1475..1972
                     /locus_tag="PKZ53_03865"
     CDS             1475..1972
                     /locus_tag="PKZ53_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNJ39602.1"
                     /translation="MSNPAMVLYGEEYQLIKTIIAKLCEDANAKMVFLVDKNGQQIAS
                     HGELGDIDTTSLASLTAGNVAATDGLARLIGEKEFPVLSHEGERDNIHISIVAQRVIL
                     VVIFDERSSLGLVRLRVRRASSELTEVFELIVRKVEQEKEKGNSLETPFAEITDEDID
                     NLFRE"
     gene            2060..2650
                     /locus_tag="PKZ53_03870"
     CDS             2060..2650
                     /locus_tag="PKZ53_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNJ39603.1"
                     /translation="MTFINYASREINCKIVYYGPGLGGKTTNLQYIYDSTAPQAKGKL
                     ISLATETDRTLFFDFLPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQYERMDANIESLWNLENNLKAQGYDLMKIPYVLQLNKRDLPGVMPIEELKKELMR
                     KGEPVFEGVAFKGVGVFDTLKAVAKQVLVELKKGGG"
     gene            2827..3600
                     /locus_tag="PKZ53_03875"
     CDS             2827..3600
                     /locus_tag="PKZ53_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNJ39604.1"
                     /translation="MLTGKKGLILGVANKRSLAWGIAQATATAGARLALTYQGDRLEE
                     NVRELAAKLEDPLVLPCDVTNDAEVATLFETLKEQFGTLDFLVHAIAFAQKEDLEGTF
                     LGTSRDGFRVAHDISAYSLTALARAAFPLMKDNGGSILTLSYLGAERAMPHYNVMGVA
                     KAALEASVRYLATDLGPSNIRVNAVSAGPISTLAARGIGGFTKMLQYHRETAPLRRNT
                     EVSEVADTALFLVSNMGRGITGEVIYVDGGFHIVGMPPL"
     gene            3655..4515
                     /locus_tag="PKZ53_03880"
     CDS             3655..4515
                     /locus_tag="PKZ53_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ39605.1"
                     /translation="MNSIRKVGVLGAGTMGRGIAQVCVQSGCDVVLHDVSGSVLEQAR
                     TAIHAGLAKAVEKGRLTPETKTAACDRLHTTSDLTLVHTADCVIEAVPEKLALKQELF
                     AELDQHLAPQAILATNTSSLSITAIAEATSRPERVIGLHFFNPVPVMKLLEIIRGKQT
                     SATVLQQALEFGKHLGKETIVVQDSPGFATSRLGLALGLEAMRMMETGVASAQDIDRA
                     MELGYNHPIGPLKLTDLVGLDVRLSIAEYLFDKLGSESFRPPQILRDLVADGKLGKKS
                     GQGFYLWNTE"
BASE COUNT         1130 a         1136 c         1170 g         1141 t
ORIGIN      
        1 accgtagcca gtgtctgggt ttctttttca attttcaccc cttctttggc ttcaactgcc
       61 tggtgcaatc catctgacca gcgacgacca gtcatcatgc gcccggtatg ttcgtcaaca
      121 atcacgactt cgccatcttt gacgatgtaa tctttatcaa ctttatagag ggtgtgcgcg
      181 cgcagggcct gattcacgca atgcaaaatt tcacggttgg ccggatcata gaggttgcca
      241 cagccgagca gtttttcagc gtgttcaatt ccatcttccg tcaatgtggc cgtgtgtgtt
      301 ttttcatcaa ccagaaaatc cgtttcgcca tttagccgtg gaataatgtc gttggcgaga
      361 tagtattttt cggttgaatc atctgaggca ccagcgatga tcagtggggt acgggcttcg
      421 tcgatcagaa tcgagtccac ttcgtccacg atggcaaagt tgtattcgcg ctggacacat
      481 tgggccaggc taaacttcat gttatcgcgc agataatcaa agccaaattc gttgttggtc
      541 ccataggtga tatcagcccc ataggcttca cggcgggtaa agtcatcaat gttgctttga
      601 atgaccccaa ctgagagtcc caggaatttg tggattcggc ccatccactc ggcgtcacgg
      661 cgagcaaggt aatcgttcac cgtcacgacg tggacacctt ttccggtcag ggcattgagg
      721 taggaaggaa gtgtggcaac cagcgttttt ccttccccgg ttcgcatttc tgcaatccga
      781 ccttcgtgca gcacgatgcc accaatcatc tggacgtcaa aatgccgcat ggtggtggtc
      841 cggcgggaag cctcgcggac agtggcaaag gcttcgggaa gcagatcgtc aagggattcc
      901 ccagcatcga gacgttcttt aaacttaaca gtttgcgccc gaagctggtc atccgtcagt
      961 tgctggattt caggttcgag gtcgttgatt cttgcgacaa tcggacgaat tcgcttaaga
     1021 taccgttcgt ttgcgctgcc aaaaattcga gtcagaagct tatcaatgat catgaaacaa
     1081 caaaatgtcg cacaagcccg ccggggctct ttttcgccgg ccagaacgag ccttgcacga
     1141 caccctcttt aaatctttaa atctccacag gttagtttca ttctcgctgg aaaccccggc
     1201 gaggtcttaa ttccccaacc gcattggtgg gaagtgaaca aattgatgat gacgaatgaa
     1261 cgggattgta ggagaagctc ccaagacacg tcaaggagcg agaaagacct tgaaagcctt
     1321 tgcctcgcta tgctataaga gactccgatt cataataact ccaataatca caaagggcta
     1381 cacagccgca aagattttcc atccaaaacc tgcaggaatt cttctgtacg gtgagcctgt
     1441 tcgtcccttt taaagcttcg aggtcaaaat agcgatgtca aaccccgcga tggtgctcta
     1501 cggcgaggaa taccaactaa tcaaaaccat catcgccaag ttgtgcgaag atgcgaatgc
     1561 caaaatggta tttcttgttg ataagaatgg acaacaaata gcttcacatg gggaacttgg
     1621 cgatattgat acgaccagtt tggcttcatt gacggcagga aacgtcgcag caactgacgg
     1681 tttggcccgc ctcattggag agaaagaatt tccggttctt tctcatgaag gcgaacgcga
     1741 caatatccac atctcaattg ttgctcaacg tgtcattctg gtcgtcatct ttgacgaacg
     1801 gtccagcctt gggctcgtcc ggttgcgcgt cagacgggct tcaagcgagc tcactgaggt
     1861 gtttgaactg attgtgcgca aagttgagca ggaaaaagaa aaaggcaact cactggaaac
     1921 cccatttgct gaaattacgg atgaggatat tgacaacctc tttcgtgagt gagtaacaat
     1981 gcctgtgttg gattcggtgg gccagttgtt cccgccgtga cgattaacca tctcttaact
     2041 actcgtaagg gttgcagcct tgacattcat taactacgca tcgcgagaaa tcaactgtaa
     2101 gatcgtgtac tacggccctg gtttgggcgg gaagacgacc aatttgcagt acatctacga
     2161 ctcgacggca ccccaggcca aagggaaact gatcagcctg gcaactgaga cggaccgtac
     2221 gctgtttttc gactttcttc cattggaact tgggacggtg cgcgggttca aaacccgttt
     2281 tcacctctac actgtgcctg ggcaggtttt ttacgatgcc agtcgcaaac tcattttgaa
     2341 gggtgtagat ggtgtggttt ttgtggccga cagccagtac gaacgcatgg acgccaacat
     2401 tgagtcactg tggaaccttg aaaacaacct caaggcccag ggttatgatt tgatgaaaat
     2461 tccctatgtc ctccaactca ataaacgcga ccttcccggc gtgatgccga ttgaagagtt
     2521 gaaaaaagag ttgatgcgca agggtgagcc tgtctttgaa ggagttgctt tcaaaggagt
     2581 tggggtgttt gacaccttga aagcggtcgc caagcaggta ctggttgaac tgaaaaaagg
     2641 cggcggctaa accaccgaac gcaggtttta ttcaaaaggc gaagggggag actcaacaga
     2701 ctctgccccg ttcgcctttt tgtgttttat tgatttcctg gctctggctt gacgttgtcg
     2761 ccaggagact gatattttcg cgctccattt cattttcatc ctcattccga gaaagtacca
     2821 actcccatgc tcacaggtaa aaaaggatta attttaggcg ttgccaacaa acgaagtctg
     2881 gcctggggaa ttgcccaggc gacagccaca gcgggtgccc ggctggcgct cacctatcag
     2941 ggcgaccgac ttgaagaaaa tgtccgcgaa ttagcggcaa aacttgagga tccactcgtg
     3001 cttccctgcg atgtgaccaa cgatgccgag gtcgccactc tgtttgaaac cctcaaagaa
     3061 cagtttggca cgctcgattt tctggttcat gcgattgcgt tcgctcaaaa agaagacctg
     3121 gaaggcacct tcctgggaac atctcgggat gggtttcgag tcgcccacga tatcagtgcc
     3181 tactccctga ccgccctggc ccgggccgcg tttccactga tgaaagacaa tggcggcagc
     3241 attttgaccc tctcctacct gggcgctgaa cgcgcaatgc cccactacaa tgtgatgggt
     3301 gtggccaaag cggcactcga agccagtgta cgctatctgg caacggattt aggcccctca
     3361 aacattcgtg tcaatgccgt ttccgccggt ccaatcagca ccctggccgc tcgtggcatt
     3421 ggtggtttta ccaagatgtt gcaatatcat cgcgagacgg cccccttgcg ccgcaacacc
     3481 gaagtcagcg aagtggccga cacagcgttg tttctggttt caaatatggg ccgtggcatc
     3541 acaggtgaag tcatttatgt ggacggcggc tttcatattg tcggcatgcc tccgctctaa
     3601 aaccatatag ggattagagt ttgaggaaaa taactctttc aatgatgtaa ctttatgaat
     3661 tccattcgaa aagtcggcgt tttaggagct ggcacaatgg ggcgtggaat tgcccaggtg
     3721 tgtgtccaga gtggctgtga cgttgtctta catgatgtct caggatcggt tctcgaacag
     3781 gcacgcaccg caatccacgc tggattggca aaggcggttg aaaaaggtcg gttaacacct
     3841 gaaaccaaaa ctgcagcctg tgatcggctt cacaccacct ctgacctgac tctggtccac
     3901 acagcagact gcgtgattga agcagtgcct gaaaaactgg ctttaaaaca ggaactgttt
     3961 gctgaactgg atcaacatct tgccccacaa gccattctgg ccaccaacac ctcttcgctg
     4021 agtatcacgg caattgctga ggcaacctca cgaccagaac gggtgattgg cctgcatttt
     4081 tttaacccgg tgccggtcat gaaactgctg gaaattattc gcggaaaaca gacttccgca
     4141 acggtgctcc agcaggcgct tgaatttggc aaacaccttg gtaaagaaac aattgtggtt
     4201 caagactcac cagggtttgc caccagccgg cttgggctgg cactgggtct ggaagcaatg
     4261 cgcatgatgg aaaccggtgt ggcgtcggca caggacattg atcgagccat ggagttgggc
     4321 tacaaccacc caattggtcc actcaaactg accgatctgg ttggtctgga tgtgcggctc
     4381 agtattgccg aatacctctt tgataaactt ggttcagagt cgtttcgtcc acctcaaatt
     4441 cttcgcgacc tggtggccga tgggaaactc ggaaagaaat ctggccaggg gttctattta
     4501 tggaatacag aatgaagaat gaagaattga gaattgagaa tgaagaaact tgttggttct
     4561 tggttcttgg ttcttgg
//
LOCUS       DAOQDO010000171         4566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12669_length_4566_cov_2.651740, whole genome shotgun sequence.
ACCESSION   DAOQDO010000171 DAOQDO010000000
VERSION     DAOQDO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12669_length_4566_cov_2.651740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKZ53_03885"
     CDS             complement(<1..645)
                     /locus_tag="PKZ53_03885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNJ39606.1"
                     /translation="MLSERIGVYGTQILDAIVRGVARLIPNPNHLTFLGLLINIWCAY
                     LYGCGLFFEAGLWMIFANLFDMVDGRVARLTNRVTSFGAFFDSVMDRYSDVIVFVGIM
                     TFYARQTEYHSTLYVALTGIALMGSVMISYTRARAESVIHQCKVGFLERPERVVLIII
                     GSLTEIGPEDNPFLHKMRAVLWILAVLSHWTVVHRMYHTLRESDRLDQLKAQPVS"
     gene            935..1159
                     /gene="rpmE"
                     /locus_tag="PKZ53_03890"
     CDS             935..1159
                     /gene="rpmE"
                     /locus_tag="PKZ53_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNJ39607.1"
                     /translation="MKEKIHPKYNECTVTCSCGNVFKTRSTKKEIRLEICSNCHPFFT
                     GKQKLVDTAGRVERFNRKYGKVPAAPPAGA"
     gene            1319..2401
                     /gene="prfA"
                     /locus_tag="PKZ53_03895"
     CDS             1319..2401
                     /gene="prfA"
                     /locus_tag="PKZ53_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNJ39608.1"
                     /translation="MLDKLNALEEKFEALTEQLNDPNVVNDMTRYPKLAKQHRELSVV
                     VEKYREFRAVQEGIESTKTLLDESDDPEMTEMAREELATLSRRVTECEQELKTLLLPK
                     DPNDDKNVILEIRAGTGGNEASLFAGELLRMYSRYAERQGWKMQILNTSESEIGGTKE
                     AIAVIEGDVVYSKLKFESGVHRVQRVPQTESSGRIHTSTVTVAVLPEAEEVDIEINPK
                     DLRVDTFCSSGPGGQSVNTTYSAVRITHIPTNTVVSIQDEKSQIKNREKAMRVLRSRL
                     LEVEQEKQQNAISSERRSQVGTGERSEKIRTYNFKENRVSDHRIGMTIHSLDLFMDGD
                     LGDVIQALITHHHAEKLKQELAEATV"
     gene            2527..2997
                     /locus_tag="PKZ53_03900"
     CDS             2527..2997
                     /locus_tag="PKZ53_03900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNJ39609.1"
                     /translation="MNDTPQLVLVVAAIIVDQERILITQRLPSGRLANQWEFPGGKVN
                     WGEPPEDALRREIQEELGLDVEVGAPVHLIHYALNTNQAFAVVFYWSRIAGGTLELIG
                     CQAARWITVNEFPKVDFLQANLPVVEILQARVAALGSLSPTFSFISCSHQGNEH"
     gene            3193..>4566
                     /locus_tag="PKZ53_03905"
     CDS             3193..>4566
                     /locus_tag="PKZ53_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNJ39610.1"
                     /translation="MELAIIIGISFVALAFAGYLIKDVLARDTGTPEMRVISDAIKEG
                     AEAFLRRQNTTIVYMAIALSALIFILYAFVRTPNQHDPVQDPRTLAFWTTLSFVLGAA
                     CSVGSGYIGMWISIRGNIRTASAATKSLNGALQTALRAGAVSGLFVVALSLFGVVGLF
                     ALVNSLKVAEITKIPLLIAGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIP
                     EDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILAAGLYTSNRGAFTDEQ
                     LLGVLLFPLVARAFGLLASIVGIMSVSTKEDGDPMAALNRGYFVTSVLAAGGFFGAAK
                     WLLNNRVAPDAYLYFFGCGLIGIVTSIAFVYLTQYYTEYKYRPVKSIAEASKTGPATN
                     IIMGTAVGFESTAFPIIVISAAIMGSYYLGKNSGLQHAGLFGTAVATMGMLGTAAYIL
                     AMDTFGPI"
BASE COUNT         1112 a         1141 c         1203 g         1110 t
ORIGIN      
        1 agagactggt tgggccttta attgatcaag ccgatctgat tcgcgaagtg tatggtacat
       61 ccgatgaacg accgtccagt gtgacaacac ggccaggatc cacaacaccg cccgcatttt
      121 atggaggaac ggattgtctt ccgggccaat ttcagtcaga gagccgatga tgatgagcac
      181 cacccgctct ggtcgttcca gaaatccaac tttacattga tgaatgacgc tctcggcgcg
      241 ggcccgagtg taactgatca tcaccgaccc catcagtgca atcccggtca gcgcaacata
      301 gagcgtgctg tgatattcgg tctgacgggc ataaaacgtc ataattccaa caaacacaat
      361 cacatctgaa taccgatcca tcactgaatc gaaaaaggcg ccaaaactgg tgacccggtt
      421 ggtcaaacgg gcgacccggc catccaccat gtcaaacaga ttggcaaaga tcatccacag
      481 accggcttca aaaaacagac cgcacccata aagataggca caccagatat taatgagaag
      541 gccaagaaag gtcaggtggt tgggatttgg aatcaggcga gccaccccac ggacaatcgc
      601 atccaggatt tgggttccat agacgccgat gcgttcgctc aacatagaaa gggatgcctc
      661 aacaaggatg attagggatg tgtgctgatt gctggatcag aataggcagt atcgagtggc
      721 gtatagtaga cgtgaagtcg ttctttgtca agaaatacac ctgatccgcc acccaagcct
      781 tgacaacaat gaaatccctg atgtacggtc cgcagcttgt agttttcgct caagttaacc
      841 ttgatttaca ccgacatcaa acagttcttg taccgaatct cacctgatga ccaggacaag
      901 ggagactgtt ttttaccaag gcgttttaca gattatgaag gaaaagattc atccaaaata
      961 caatgaatgc actgtgacct gttcctgcgg aaatgtcttc aagacccgtt caacgaagaa
     1021 agaaatccgg ctggaaattt gctcaaactg ccacccattt ttcaccggca aacagaagct
     1081 ggttgacacc gctggccggg ttgaacgctt taaccgcaaa tacggcaaag tacctgcggc
     1141 cccacctgcc ggcgcctagc tgccgagccc gtcttcatcc agaaccaccg ccagccccat
     1201 cgtcattctc actgatcggt aagggtgacc ctcgaagcga attgcggtgg tttctttttt
     1261 gtctggctta caccctgaag ttgacaacca gtgtaggcaa attggtggag gaaagacgat
     1321 gctcgataaa ctcaatgccc ttgaagagaa atttgaagcg ctcaccgagc aactcaatga
     1381 cccaaatgtg gtcaatgaca tgacccgcta tcccaaactc gccaaacaac atcgcgagct
     1441 aagtgtggtg gttgaaaagt atcgggagtt tcgcgctgta caagagggaa tcgaatcaac
     1501 aaagacactt ctggacgaat ctgacgatcc ggaaatgacg gaaatggccc gggaagaact
     1561 ggcaaccctt tcccgccgcg tcactgagtg tgagcaggaa ctcaaaaccc tgctcctgcc
     1621 gaaagatcca aacgacgaca aaaacgtgat tctggaaatc cgcgccggta ctggagggaa
     1681 cgaagcgtcg ctgtttgctg gggaattgct ccgcatgtat tcccgctatg ccgaacggca
     1741 agggtggaaa atgcagattt tgaatacttc agaatctgaa atcggcggca ccaaggaagc
     1801 gattgccgtg atcgaaggtg atgtggtgta ttcgaaactg aaatttgagt ccggggttca
     1861 tcgcgttcaa cgggtgcctc agaccgaatc ctcaggccga attcatactt cgacggtgac
     1921 ggtggcggtg ctgcctgaag ccgaagaagt tgatattgaa atcaacccaa aggatttgcg
     1981 ggttgatacc ttctgttcct caggtccagg tgggcagtcg gttaacacga cctactcagc
     2041 cgtccggatt acgcacattc caaccaacac ggtggtttcg atccaggatg aaaaatccca
     2101 gatcaaaaac cgtgaaaagg ccatgcgcgt cttgcgatcc cggttgctcg aagtcgaaca
     2161 ggaaaaacag cagaatgcca tttcttcgga gcgtcggtca caggttggaa ccggtgagcg
     2221 gagcgagaag attcggacct ataatttcaa ggaaaatcgg gtttccgatc accgtatcgg
     2281 aatgacgatt cattcacttg atctctttat ggacggtgat ctgggggatg tcattcaagc
     2341 cctgattaca caccatcacg ctgaaaaatt gaaacaggaa ctggccgaag caacagtgta
     2401 atgctggtgc ccggccagtt gtctttcggg atcgttggtg gttggtacct gggtatggat
     2461 tttctcttct ttcaaagaat tcatgtccgg gtaccagtca ccaaataaca ggcacttctt
     2521 ttgccgatga atgacactcc tcaactcgtc ctggtggttg cggcaattat tgttgatcag
     2581 gaacgcattt taatcaccca gcggcttcca tctggacgac tggcgaatca atgggagttt
     2641 ccaggcggca aggtgaactg gggagagcct cccgaagatg ccttgcgtcg ggaaattcag
     2701 gaggaactgg gacttgatgt cgaagtcggc gcgccagtgc acctgattca ctatgcgttg
     2761 aacacaaatc aggcgtttgc agtcgttttt tactggtctc gaatcgcagg gggcacgctc
     2821 gaactcattg gatgtcaagc tgcccgctgg atcacggtga atgaatttcc aaaagtggat
     2881 tttctgcagg ccaatctccc ggtcgtggag atactgcagg ccagggtcgc agctctggga
     2941 tcactttcac ccactttttc attcatttct tgttcccacc aggggaatga acactgactt
     3001 tgaaccgacc ttttcggtga aacttccatt cagttgtctt gagcgtgccc cttttgctcg
     3061 aatcacaccc aaattcttga tcctgataag cttcaatgcg tcagtgaggc ttgaatcaaa
     3121 ccactcttct catttttgtc gaaaggagtt tgatgtcaca ggtggctctg gaagagctga
     3181 catctcaaca ctatggaact ggcaattatc attgggatta gcttcgtggc cctcgccttc
     3241 gcaggctatc tcattaaaga tgtactcgcg cgcgatactg gcacgcccga aatgcgtgtc
     3301 atctccgatg caattaaaga aggtgccgaa gcctttttgc gccgtcaaaa cacaaccatt
     3361 gtgtatatgg cgattgcgct gtcggccctg attttcattc tgtatgcgtt tgtgcggacg
     3421 ccaaaccaac atgatccggt tcaagatccc cgcaccctgg cgttttggac cacgctctcc
     3481 tttgttctgg gggctgcctg ttcggttggc tctggttaca ttgggatgtg gatttccatc
     3541 cgtggtaaca tccgcactgc ttcagccgcc accaagagtc tcaatggtgc ccttcaaact
     3601 gccttgcgcg ctggcgcggt gtcagggctg tttgtggtgg cactctcctt atttggggtg
     3661 gtgggattgt ttgcgctggt caattccctg aaggtcgcgg aaataacaaa aatcccgctt
     3721 ctgattgctg gatatggatt tggcgcttca tttgtggcgc tcttcgccca gttgggtggt
     3781 gggatttata ccaaagcggc tgacgtcggt gcggacctgg ttggtaaagt cgaagccggg
     3841 attccggaag acgacccacg caacccggcg gtgattgcag atctggttgg cgacaacgtg
     3901 ggcgactgtg ctggtcgtgg agctgacttg tttgaatcaa ccgcggctga aaacatcggt
     3961 gcgatgattt tggcggcggg gttgtatacc tcaaatcgtg gtgcctttac cgacgagcag
     4021 ctcctgggcg tgttgttgtt cccgcttgtg gcgcgcgcct ttgggttgct ggcctcgatt
     4081 gtgggaatta tgagcgtcag caccaaggaa gatggcgacc caatggctgc cttaaaccgg
     4141 gggtactttg tgacctcagt gctggccgcc ggtggatttt tcggtgccgc gaaatggttg
     4201 ctcaataacc gggtggcacc agatgcctat ctgtatttct ttgggtgcgg tttgatcgga
     4261 attgtaacct caattgcctt tgtgtatttg acccagtact acacggagta caaataccga
     4321 ccagtgaagt ccattgctga agcttcaaaa actggtccgg cgaccaacat cattatggga
     4381 acggcagttg ggttcgaatc aaccgcgttt ccgatcattg tgatttcagc cgcgatcatg
     4441 ggctcctact acctgggcaa aaactccggg ttgcagcatg ccggcttgtt tggtactgcc
     4501 gtggccacga tggggatgct cggtacggca gcctacattc tggcgatgga tacctttgga
     4561 cctatc
//
LOCUS       DAOQDO010000172         4565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12673_length_4565_cov_3.401330, whole genome shotgun sequence.
ACCESSION   DAOQDO010000172 DAOQDO010000000
VERSION     DAOQDO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12673_length_4565_cov_3.401330"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKZ53_03910"
     CDS             <1..291
                     /locus_tag="PKZ53_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ39611.1"
                     /translation="FPEGRRSEEGHIHPFRSGIGVLAKEIQVPVLPVYLAGLYEQKQK
                     GKAHWFNRFWAAPGSVQIRFGPPVTFSADAPPEHITQELEQAVRQLEPSKSI"
     gene            complement(296..856)
                     /locus_tag="PKZ53_03915"
     CDS             complement(296..856)
                     /locus_tag="PKZ53_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39612.1"
                     /translation="MSTHPTKRWFSVQDFHRMVETGILTEDDRTELIAGVVISMSPFG
                     SRHAACVKRFNALLSRQLLGKVIIGVQDPIQLDSFTEPQPDLSILQFRDDFYAEQHPR
                     AREVLLLIEVSDLSLSYDRDTKLPLYAQAEIPEVWIVDLTAEKILAYQQPKAGKYEVQ
                     ETYTAATKLTSVAGFPVEINLSTIFD"
     gene            complement(925..3024)
                     /locus_tag="PKZ53_03920"
     CDS             complement(925..3024)
                     /locus_tag="PKZ53_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine carboxypeptidase"
                     /protein_id="HNJ39613.1"
                     /translation="MKKILVIHTNQEHRQETIEFLGETIEIERIGSEGDPEKVQELIS
                     QFDGKVDAIALEGMPAILELGSARRPHSKGTALAKAATQTPVVDGHGVRSGLERWGVI
                     LANRAQPGIFSYKRILMFPGLNHTGLAHALGRNGSEIRYGDPIMFFNLPALPGVGAKY
                     TLEQAAPTTLDQLKDAPFRRLQPSPGEPHAKRTTQIFDWAEVIAGDIGAILRYAPKTL
                     KRKTVVVENASPKQVEELRERGVSIVVTLMPSLSKNDALGCWSAATLEAVLASLRTKT
                     DSPLDENTYLDLLAEIQWTPSIQYLQPDEAGINRFAFVIHPLDISFIHAHPTFQWTRL
                     FPNSLVERLAAYMPPMYISRITGGISPTTGQRIEGYLITLSATPRQMMRMDPRFTYKQ
                     LNRAARMAERLGARIMGLGAFTSVVGDAGMTVAHEADIAITSGNSLTVAATLEAAKQA
                     VIKMGVKDLTRGKAMIIGATGSIGSVCSRLLAQAIHDVVLVSIEPERLIDLKRSIQEE
                     TPEAKVAIATRPDELLPECDLIVTATSAFGQRIVDITKCKPGAVICDVARPPDINKRE
                     GALRPDVLVIESGEVLIPGDIDFGYNIGLPPKTAYACLAETSLLAMEGRFEDYTLGRN
                     ITIDRVKEIYRLFKKHGYQLAGLRSFGKYITDEDVAKKRTLAETWRKDPEGFARMQIE
                     AAEKLAKIPVMAKGVSG"
     gene            complement(3426..3569)
                     /locus_tag="PKZ53_03925"
     CDS             complement(3426..3569)
                     /locus_tag="PKZ53_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39614.1"
                     /translation="MPICLSFDLPHTSLSHKARWKKHSVANLASVSASPVPHRNASQR
                     QFD"
     gene            3723..4412
                     /locus_tag="PKZ53_03930"
     CDS             3723..4412
                     /locus_tag="PKZ53_03930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ39615.1"
                     /translation="MKFLNLDDLEQSSIVANSLMNRERNVAGTNSYQKDLRFDVLNFI
                     TTELSRKPTMSWLDLCCGTGRALLQSAQKLNEAGWAEKVSLVGVDLVDGFFPKPESFY
                     FLELVPASLTNWHPAQPFDLITCVHGLHYIGDKLDVVSRMASWLTTDGMFLANLDLNN
                     IKLDQPRLSNRYLISLLTNKGLHYDARRKILTCTGQKHISFELIFLGADDQAGPNYTR
                     QPAVNSWYRSI"
BASE COUNT         1081 a         1141 c         1145 g         1198 t
ORIGIN      
        1 tttcccgaag gtcgccgatc agaggaaggg cacatacatc cgtttcggtc agggattggt
       61 gtcctggcca aagaaatcca ggtgccggta ctgccggttt acctggccgg cctgtacgaa
      121 caaaaacaaa aaggcaaagc gcactggttc aatcgctttt gggcggcacc cggttcagtt
      181 cagattcgat ttggcccacc agtgacgttt tcagccgacg cgcccccgga acacatcacc
      241 caggagcttg aacaagccgt ccgtcagctt gaaccatcaa aatccatttg attcctcaat
      301 caaaaattgt tgacaggtta atttcgactg gaaagcctgc gactgacgta agtttggtgg
      361 ctgccgtata cgtttcctgg acttcgtatt tcccggcttt tggctgctgg taagccagga
      421 ttttctcagc cgtcaaatca acgatccata cttctggaat ctcagcctga gcatacaatg
      481 gcaacttcgt gtcgcggtca taactgagtg aaaggtctga gacttcaatg agcagcaata
      541 cctccctggc tcgcggatgt tgctcagcat agaagtcatc tctgaattga agaattgaaa
      601 gatcaggttg gggttcagta aacgaatcca gttgaattgg atcctggaca ccaataatca
      661 cttttccaag tagttgcctc gaaagcaagg cattaaaccg tttgacacag gcagcatgcc
      721 gacttccaaa gggactcatt gaaatgacca cgccagcaat cagttcggtt cggtcatctt
      781 cagtcaagat tcccgtttca accatccggt ggaaatcctg aactgaaaac cacctttttg
      841 tgggatgtgt tgacatataa acctcctttg gagtgcggcg gcttgacgcc gctttgaact
      901 cccctcgcgt tctttcagcc acgtctaccc gcttacccct ttcgccatga ccggaatttt
      961 ggctaatttc tcagccgctt ctatctgcat gcgggcaaaa ccttccgggt ctttgcgcca
     1021 ggtttcagcc agtgtccgtt ttttggccac atcttcatct gtgatgtatt ttccaaacga
     1081 acgcagtcca gccagttgat agccgtgttt tttaaacagg cgatagattt ctttgactcg
     1141 atcaatggtg atgttgcgac ccagcgtgta gtcctcaaat cgtccttcca tggccaaaag
     1201 ggaagtttcc gccagacagg cataggcggt tttcggcgga agcccgatgt tatagccaaa
     1261 atcaatatca ccggggatca agacttcacc actttcgatg accagcacat ctggcctgag
     1321 ggcgccttcc cgtttgttga tatcgggtgg gcgagcaacg tcacaaatga cggcgccggg
     1381 cttacatttg gtaatatcca caattcgctg cccaaaagct gaagttgctg tcacgatcaa
     1441 atcacattct gggaggagtt catccgggcg cgtggcaatc gccactttgg cttccggggt
     1501 ttcttcctga atcgagcgtt ttaagtcaat caggcgttca ggttcgatag agaccagcac
     1561 cacatcatga attgcctggg ccaggagccg tgaacacacc gaaccaattg aaccggtggc
     1621 accaatgatc atggcttttc cacgcgtcag gtctttgacg cccatcttaa taacggcctg
     1681 tttggccgct tcgagcgtgg ccgcgacggt caaactgttg ccgctggtaa tggcaatatc
     1741 ggcttcgtgg gccaccgtca tcccggcatc cccaaccaca ctggtaaacg ctcctaaccc
     1801 cataatgcgc gcacccaggc gttcggccat gcgggccgcc cgattgagtt gcttgtaggt
     1861 aaatcgcggg tccatgcgca tcatctggcg cggcgtcgct gacagcgtaa tcaaatatcc
     1921 ttcaatccgc tggccagtgg ttggtgaaat cccccctgtg atccgggaga tatacatcgg
     1981 aggcatatag gcggctagtc tttcaaccag tgaattcgga aagagtctgg tccattgaaa
     2041 ggttgggtga gcgtggataa aactgatgtc gagcggatga atcacaaacg caaaccgatt
     2101 gatcccggct tcatctggct gaaggtactg gattgagggc gtccattgaa tttcagccag
     2161 caaatccaga taggtatttt catctaacgg ggagtctgtt ttggtccgca acgaagccag
     2221 caccgcttca agtgtcgctg ccgaccagca gcccaacgcg tcattttttg agagcgatgg
     2281 catcaaggtc acgacaattg aaacgccacg ttcgcgcaat tcctcgactt gtttcggtga
     2341 ggcgttttca accaccacgg ttttgcgttt caacgtcttt ggggcatagc gcaggatggc
     2401 accgatatca ccggcaatga cttcggccca gtcaaagatt tgggttgttc gtttggcatg
     2461 tggttcgcct ggggaaggtt gaagccggcg aaatggcgca tcctttaatt gatcaagtgt
     2521 ggttggcgcg gcctgttcca gggtgtattt ggcgccgact ccggggaggg ccggaagatt
     2581 aaaaaacata attggatccc catagcggat ttcagatcca tttcgcccta acgcatgagc
     2641 cagcccggtg tggtttaaac ctggaaacat caaaattcgc ttgtaactga aaattcccgg
     2701 ttgtgcccgg ttcgccagaa tgacacccca ccgttccaac ccagagcgaa cgccgtgacc
     2761 atcaacgact ggggtttgtg tggcagcttt ggccagcgct gtacctttgg aatgcggtcg
     2821 gcgagctgag ccaagttcca ggatcgccgg catgccttcg agcgcgatgg catcaacttt
     2881 cccatcaaac tgggagatca attcctggac tttctctgga tcaccttccg acccgatgcg
     2941 ttcaatttca attgtttctc caagaaattc gatggtttcc tgacggtgtt cctgattggt
     3001 gtggatgacg aggatctttt tcatggcagt ggtttcgctt ttcaactcaa gaggtgaaga
     3061 cgtgcggttc aaacattcca ggtctggggc aacccaattt tttcacttga ccatgcagga
     3121 aacaattttc aggccacaaa tcagcaagtc tgtgagtttc tgggagaaaa tcagttgaat
     3181 cagacgtccg ttttccatcc aggaatcaac tttctggctg gtgaatcaaa ctgctggctt
     3241 cactctgtgg tggttggttc cccgtcgagt cactttttca ttttctgaaa acccaatcca
     3301 gatatgtcat ttccaacatc tggaagttag accttctttg atgtgaattg tgggtgtata
     3361 gtggtgtgat gactatctga aagttgaatt tctacagcac tttggaataa tctcgatcat
     3421 aaacgtcaat caaattgcct ttgactcgca ttgcggtgtg gtactggact ggctgaaaca
     3481 ctggccagat tggcgacgct gtgctttttc catctggctt tgtgactcaa actggtatgt
     3541 ggcaaatcaa atgaaagaca gatcggcact cgaattacct gaattcttta tgtctgaatt
     3601 ttcgtctttc agttcatcac tcgcggaatg actggtcaga tcaccggtga agatacccga
     3661 ggctgaggaa tttttcccac tgcctcccgt atgatttcga ttttcctgat tccggaataa
     3721 agatgaaatt tttaaacctg gacgatttag agcaatcatc aattgttgcc aattcactga
     3781 tgaatcgtga acgaaacgtc gcgggcacca atagctatca aaaagacctg cggtttgatg
     3841 tgctcaattt cataaccacc gaactctctc gaaagccaac gatgtcgtgg cttgatctgt
     3901 gttgtggaac gggaagagcg ctgctgcaat cagctcagaa attgaatgaa gccgggtggg
     3961 ctgaaaaagt aagcctggtg ggtgttgacc tggtggatgg atttttcccc aagccggaat
     4021 ccttttattt tctggagctg gttccggctt cgctcacaaa ctggcatccg gctcaaccat
     4081 ttgatctgat tacctgtgtg catggattgc actatattgg ggataaactg gatgttgtga
     4141 gccggatggc atcgtggttg accacggacg ggatgtttct ggccaacctt gatctcaaca
     4201 atatcaagct ggaccagcca cggttatcaa atcggtacct gatctcattg ctcacaaaca
     4261 agggattgca ctatgatgcc cggcgaaaaa tcctgacctg tactggccaa aaacatatca
     4321 gttttgaact gatatttctc ggagctgatg atcaggccgg gccaaactac accaggcagc
     4381 cggcggtcaa ttcctggtac cggtcaatat agtttctttt gaggttggca aacgaagaaa
     4441 agccagctca ggtgggtgcc gtcaagcgct tcatccgcct gggttatgta atggatttgc
     4501 tgatttcttc actccagacg aacccgaagc ctgtcagcca gcctgacggt gatttccggt
     4561 tggtt
//
LOCUS       DAOQDO010000173         4564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12680_length_4564_cov_3.359503, whole genome shotgun sequence.
ACCESSION   DAOQDO010000173 DAOQDO010000000
VERSION     DAOQDO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12680_length_4564_cov_3.359503"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..942)
                     /locus_tag="PKZ53_03935"
     CDS             complement(70..942)
                     /locus_tag="PKZ53_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNJ39616.1"
                     /translation="MTKPQLPPPKPKPRRHRLDQWLVEHNLAESRHKAQALILAGQVL
                     INEQRAEKPSQVVTDQTSVRIRGEGLRYVGRGGLKLEGALRTFGLVVDQAVCLDVGAS
                     TGGFTDCLLQHGAARVYAIDVGTNQLVWKLRTSPQVIVREQVNARYLQPSDFPELFDV
                     VTCDVSFISQDKILPALGPLMKTGAFLVTLVKPQFEVGREDVGKGGIVKDPFLHRRAI
                     EKVAQAAQTAGIQPWHVMCSPIEGADGNREFLLIGKRLEVEPVSLSSDIDLTGWQTEL
                     NALFQIDQGRKNEE"
     gene            1145..1678
                     /locus_tag="PKZ53_03940"
     CDS             1145..1678
                     /locus_tag="PKZ53_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39617.1"
                     /translation="MNYLLRAVLGIGLGVSACLSSGCTLPGDSTSKPAATPKENSPSS
                     SPATPSGQSPTPAGKEAESPTSPTSKGPAKLPTDVPFYSGGAITSETANGDSYVLTYT
                     APTASKTVSSFYESELKKQGWSVKASTSVEVPFLGASVAPMVAEKGERSCSVTISGKN
                     DSECSVVLVVGTKQASK"
     gene            complement(1877..3046)
                     /gene="lpxB"
                     /locus_tag="PKZ53_03945"
     CDS             complement(1877..3046)
                     /gene="lpxB"
                     /locus_tag="PKZ53_03945"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNJ39618.1"
                     /translation="MSNSGTALPPKILMVAGEASGDMHAADLVYHLRQLSMAASTTPP
                     QFYGMGGGALRELGLAPLLPMEPVSVIGLVEVLRHLPQIWTTFQGLVRSLDQEHPTLA
                     VLVDFPDFNLRLAKKIKQRGIPIVWYISPQLWAWREHRVHTFKRIVDRMIVLFPFEVE
                     FYRKYDMEVTFVGHPLVDRVRVTRSRLEVRQELGLRDDQRVVALLPGSRRSELKMYLE
                     PMLAAAERLAHHNPQLHFVLPLARSLQPADLAPYLQRYRVPLTWRQGLSYELTHIAEA
                     AVVASGTATLETALIGTPMIILGRVSRLTELWLRRVANLPYYGLVNFVMGEKCVTELI
                     QQSVTPENIVTDVIRLLEDSAEQARLQACYTEIRHRLGDGGAAQRAASAIWEMVI"
     gene            complement(3039..3524)
                     /locus_tag="PKZ53_03950"
     CDS             complement(3039..3524)
                     /locus_tag="PKZ53_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNJ39619.1"
                     /translation="MSQTMKKLILVEDLVSQVAGLREQGHRIILANGCFDLIHVGHIR
                     YLQGAKALGGILVVGVNGDCAVRQLKGAGRPVMPDIERAEIVAALACVDFVTIFDTLT
                     VHPLLEALRPDVHAKGTDYTTETVPEREFVRSYGGQVAIVGDPKDHSTTELLQKTAAH
                     E"
     gene            3771..4070
                     /locus_tag="PKZ53_03955"
     CDS             3771..4070
                     /locus_tag="PKZ53_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39620.1"
                     /translation="MTQRQIFTGFIHLLLLGTLVASATPQTLAAQSQEQVTILFQNKD
                     KDKNKDKDKEKPKDKAPAQNFTNQDMGKQPRFIPKVPKQNPEPPQNDPPKDKKKG"
BASE COUNT         1168 a         1244 c         1073 g         1079 t
ORIGIN      
        1 ccaagaacca agaaccaaga accaagaacc aatcactaac cgcattcttc attcttcatt
       61 cttcattctt cattcttcat tcttccggcc ctggtcaatc tggaaaagtg cattcaattc
      121 agtctgccac cccgtcaaat caatgtctga tgacaggctc accggttcaa cttccagacg
      181 cttcccaatc aagagaaact ctcggtttcc atcagctcct tcaattggcg aacacatcac
      241 atgccagggt tgaattccag ccgtctgtgc tgcctgggca actttttcga ttgcccgtcg
      301 gtgcaagaac ggatctttca caattccgcc ctttcccaca tcctcacgac caacctcaaa
      361 ttgtggctta accagcgtca ccaggaaggc accggttttc atgagcggac caagtgctgg
      421 aaggatttta tcctgtgaaa taaacgacac atcacaggtc acaacatcga aaagctcagg
      481 aaaatcagat ggttgcaaat atcgggcatt cacctgttca cggacaatga cctgtggtga
      541 agtgcgtaat ttccagacca actggttggt tccgacatca attgcataga ctcgtgcggc
      601 cccgtgttgc aacaagcaat cagtaaaacc accggttgaa gccccaacat cgagacacac
      661 cgcctggtca accaccagac caaacgtacg cagggcacct tcaagcttga gtccgccgcg
      721 tccaacatag cgcaaccctt ctcctcgaat cctcacactg gtctgatcgg tcacgacctg
      781 gctcggtttt tccgctcgct gctcgttgat caggacctga cccgccaaaa tgagtgcctg
      841 cgctttgtgc cgtgactcag ccaggttgtg ttcaaccagc cattgatcca gccggtgtcg
      901 cctgggcttg ggttttgggg gtgggagttg gggtttggtc ataggacagg tagtgaggta
      961 gcaaaatagc cagatcgtgg aataaataaa ccagtacttg atcgcttgaa aagatgattt
     1021 tgatgaacgc cttaaaaacc tgttttcagc tcgctcaagg gttgagaaat caggataatc
     1081 aacttctcaa tccttccagg gtgtatgtgt ccaggtttat tcctcatcat ttggagagat
     1141 ctctatgaat tatttgcttc gtgccgtact tggtatcggg ttaggtgtga gtgcttgtct
     1201 cagttccggg tgtaccttgc cgggtgattc cacttcgaaa ccagcggcaa cgccgaagga
     1261 aaattcgcct tcatcctcac cggcgacccc atctggccag tcgccaacgc ctgccggcaa
     1321 agaggctgaa tccccgactt cccccacatc aaaaggaccg gcgaagctcc caacggatgt
     1381 tccgttttac agtggaggcg ccatcaccag tgaaaccgca aacggagatt cctatgtcct
     1441 cacctacaca gctccaactg caagcaaaac cgtttcaagc ttttatgaaa gcgagctgaa
     1501 gaaacagggg tggtcagtga aagcctcaac cagtgttgaa gttcccttcc tgggtgccag
     1561 tgtggcgcca atggtggccg agaaagggga gcgatcctgc agcgtcacca tcagtgggaa
     1621 aaatgattca gagtgtagcg tggtgctggt ggtgggaacc aaacaggcat cgaaataata
     1681 ccagtttgca gtcagtagtc agtagtcagt agttcgctaa gttcctttga ttgaattatt
     1741 tgactgtttc ctgttttagg gcttggggct gaagactcgc aagctcgggg caatggaagg
     1801 gatgaaagaa aaccagttct tcagcccaac ttcttcagcc ctcagccctg gttttttcag
     1861 cccgatgtct tcaaccctaa atcaccattt cccagatggc actggcggct cgctgtgcgg
     1921 caccgccatc gcccaaccga tgccggattt cagtatagca agcctgcaat cgagcctgtt
     1981 cggcactatc ctcaagcaac cgaattacat cggtgacaat gttttctgga gtcacagact
     2041 gctggatcag ttcggtgacg cacttttcgc ccatcacaaa gttgaccagc ccgtaatatg
     2101 gcaaattggc tacccgcctg agccagagtt cagtcaatcg tgaaacccgc cccaaaataa
     2161 tcatcggggt cccaatcagt gccgtttcca gcgtggctgt tccagacgca acaactgccg
     2221 cttcagcaat atgggtcaac tcataggaca gtccctgccg ccaggtgaga ggaacccggt
     2281 atcgttgcaa ataaggagca agatccgctg gctggagcga acgagccagt ggtaaaacaa
     2341 aatgaagctg aggattatgg tgtgccaggc gttcggcggc ggccagcatt ggctctaaat
     2401 acattttcaa ttctgagcgc cggcttccag gcaataaggc caccactcgc tggtcgtccc
     2461 tcagacctaa ctcctggcgc acctcaagcc gcgagcgtgt cacccggacg cgatccacca
     2521 gtggatggcc aacgaaggtt acctccatgt catattttcg ataaaactcc acttcgaacg
     2581 gaaacaacac gatcatccgg tcaacgatcc gcttaaacgt atgaacccga tgctcgcgcc
     2641 atgcccagag ctgagggctg atataccaga caatcggaat gccccgctgt ttgatttttt
     2701 tcgccagccg cagattgaaa tccgggaaat caaccagcac ggccagggtc ggatgctcct
     2761 gatccagtga tcgaaccaga ccttgaaacg tggtccagat ttggggcaaa tggcggagca
     2821 cctcaaccag accaatcaca ctcactggct ccattggcaa aagcggcgcc aggccaagct
     2881 ctcggagcgc cccgccgccc attccgtaaa attgaggagg ggtcgttgac gcggccattg
     2941 aaagctgtct cagatgataa accaggtcag cggcgtgcat atcgccggag gcttcgccag
     3001 ccaccatcaa gattttgggt ggtaaagcgg tgccggaatt actcatgagc ggctgttttc
     3061 tggagcaatt cggtggttga atgatccttt ggatcgccaa caatcgcgac ctgcccacca
     3121 taactgcgga caaattcccg ttctggcacg gtttcagtcg tgtaatcagt ccctttggcg
     3181 tgcacatcag gccgtaaagc ttcgagcaat ggatgaacgg tgagcgtatc aaagatcgtc
     3241 acgaaatcca cacaggccag ggccgccacg atttctgccc gttcgatatc gggcatcacc
     3301 gggcgaccgg cacctttgag ctggcgcacc gcacaatctc cattgacgcc aaccaccaga
     3361 attccaccca gtgccttggc gccttgcaaa tagcggatgt gaccaacgtg aatcaaatca
     3421 aaacacccgt tagccaggat gattcgatgt ccctgctctc gcaggcccgc gacctgggaa
     3481 accaggtcct ccaccaaaat cagtttcttc atggtttggg aaatttccga cacagacttt
     3541 ctccaaaaat atatggacag ctcgctctca cattccatat ggtgtaacgt acagccgaaa
     3601 gcggttaccc gttgaccagc gtgaggcttg attcctcggg atattttcgt tttaaaggct
     3661 ttattttcac atttctgata gccagcccct tttatttgct ggtgatgacg ctttcaggca
     3721 caagaattgt atatcaaaag gggagagttc tttggaacag tgaggatgtt atgacacaac
     3781 gacagatttt tacaggtttc attcatttac tgcttctggg caccctggta gccagtgcaa
     3841 ctccccagac actggcggct caatctcagg aacaggtaac tattttattt cagaacaagg
     3901 ataaagacaa aaataaggat aaagacaaag agaaacccaa agacaaggcg ccagcccaaa
     3961 atttcaccaa ccaggatatg ggaaaacaac cccggtttat tcccaaagtc ccgaaacaaa
     4021 acccagagcc gccccaaaac gatccaccta aggataagaa aaagggctaa cttcgtcacc
     4081 aatcgaactc aagccaccgg gcatcccctc tcaaacaggg gatgcccggt ttgctttttg
     4141 ctggcagcca cttgttttcc agcctgtttt tgcctgcttt ctgattttaa tagtgcttga
     4201 cagcataaaa agcttcttgt actctcggca cgtctttgtg aagcatcatt tcttccaata
     4261 gttttgttcg taaggtagtc ccgatttgtc cccctccata agcacgcaga aatgaaccgc
     4321 ttgttcaggc aataaatttg cttgaagagc ggtctttttt gtttttttgc cctggaagat
     4381 caggttggga atccacacct catagcttca gttgtatgca gttacctccc aagcaatctg
     4441 cacttcgact gggcctgaaa ccaacaggct ggcggcagaa ccctgtcttt acaccgacat
     4501 atcttgaaac ctaaaataat tgatttacct acgtttagga ggaagtgtta cacaatgcgt
     4561 acca
//
LOCUS       DAOQDO010000174         4538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12796_length_4538_cov_4.196074, whole genome shotgun sequence.
ACCESSION   DAOQDO010000174 DAOQDO010000000
VERSION     DAOQDO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_4538_cov_4.196074"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PKZ53_03960"
     CDS             complement(<1..1019)
                     /locus_tag="PKZ53_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ39621.1"
                     /translation="MTIVYSARWVLPVSVPPIPYGAVAVHNGVITAVGPEEVVRESVA
                     KDGPYQTVSLGETALTPGFVNVHSHLELTALRGLLDELPFYPWITKLVELKREKLRAA
                     DFQIAARWGVIEAIRSGITTLADTGDSGAPMDAMVTGGVRGIVYQEVFGLEMLKAKRS
                     LGDLKRKIQTLRRHETSLVKVGVSPHAPYTVSKLLFQLTTEFAEQDKLPMCIHTAESR
                     AEEDFMRQGLGPIGDSYRRRDIQWSPPGVSTIRYLADLNVLRVKPLLVHCVRATEEDI
                     GLLALWDARVAHCPKSNAKFGHGIAPALRMRRRGIPVGLGTDSVASNNVCDLLDEARF
                     TGLLHR"
     gene            complement(1278..2591)
                     /gene="amrB"
                     /locus_tag="PKZ53_03965"
     CDS             complement(1278..2591)
                     /gene="amrB"
                     /locus_tag="PKZ53_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNJ39622.1"
                     /translation="MYSNLHFPLPERPRLRQVQLIPAEAEAERVFIAHDPQNYTEPVI
                     IPEALGLFLLFCDGQHTVETMQQAIRFQAGVTIPQERIIQILEMFDSFLLFDSARFQQ
                     FQSKVDAEFIASPVRPCAHAGASYPADTEALGLRLDEILAGGPPVEARTDSRLVGVIS
                     PHIDLRVGGAVYAPAYRILGESLAHLEDDPTAQLTFIILGTSHYGGNGLFVASRKAYE
                     TPFGQLQCDVAFLEHLERQLGQSISHDDTSHRHEHSIEFQVIFLQHLFPKAVAQGRVR
                     MVPILCTSFQMLLNESDVAGNPDRVEYQDFVKALRVTLQSRPQSSCLLVGGDLAHIGR
                     KFGDPFDAEEKLEAVAQADSALLSVVEQRDAQALLTHIAQDQDARNVCGFPPMLTFLD
                     ALDVFGDCEGEVLHYEQWHEVERASAVTYASLAFWQIPGADNGAQ"
     gene            complement(2749..3396)
                     /locus_tag="PKZ53_03970"
     CDS             complement(2749..3396)
                     /locus_tag="PKZ53_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNJ39623.1"
                     /translation="MARSLKRQLLSFTLFVLVVSASFFGYTYWRIHRFDKLIAKTATE
                     YKLEERLVRAIVYEESYFNPRARSGAGAVGLMQVTPVVITEWKQKRREQNASTNFARE
                     MARRVPNLSSSQASKLTEDDLLQYPEINLAIGCWYLDLLRQRYQAETIVVPMMLAGYN
                     AGPSQAERWRAATAQAGKNQLTADTYVAQIDYPETRNYVRRILDRYQNKRGMLEF"
     gene            3637..4500
                     /locus_tag="PKZ53_03975"
     CDS             3637..4500
                     /locus_tag="PKZ53_03975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNJ39624.1"
                     /translation="MGKIGLLAMDVDGTLLTPDGKVTDRTRQALEQAERAHQVHLVLA
                     TGRRFHAVRPVAQNLGIRTPLITHNGALIKNLETRSIYRYHWLENDIARRLIELGKQC
                     GADTLALEDPDGDGRILTDSVSENNAPLKRYLEMNRAYVRPVPNLLHEVTGPVIQVMF
                     CGPCRPMAELAEILLAEMSEVTRLLMTAYPRNDMTILDLLNPAASKGTALEFVAEFFE
                     IDQSDVMAVGDNHNDIDMLQYAGIGVVMGNAEPELLEMGFHRTASNTEDGLAKAVERF
                     ILGLELGRKEE"
BASE COUNT         1187 a         1166 c         1166 g         1019 t
ORIGIN      
        1 cgatggagca atccggtaaa gcgtgcttca tcaagcaaat cacagacatt gttgctggca
       61 acgctatccg ttcccaaccc aaccggaatt ccccgtcgtc gcatgcgcag ggctggggca
      121 atgccatgac caaacttggc atttgatttt ggacaatggg caacacgggc atcccagagg
      181 gcaagcaacc cgatgtcttc ttccgtggca cgaacacagt ggaccagcaa tggctttacc
      241 cgtaacacgt tcagatcagc cagatagcgg atggttgaaa ccccaggtgg cgaccattgg
      301 atatcacggc ggcgatagga atcaccaatc ggccccaatc cctggcgcat aaaatcttct
      361 tcagcccgag attccgccgt gtggatgcac atcgggagtt tgtcttgttc ggcaaattca
      421 gtcgtgagtt gaaacaacaa ttttgaaaca gtgtatgggg catgcggaga aactccgact
      481 ttgaccaacg atgtttcatg ccggcgcaag gtctggattt tgcgcttcag atcgcccagg
      541 ctccgtttgg ccttgagcat ttcgaggcca aagacttcct ggtacacaat gccacggaca
      601 ccgccagtga ccatggcatc catcggggcg ccagagtcgc ccgtatcggc cagcgtcgta
      661 atgcctgacc gaatggcttc gatcacaccc cagcgcgctg caatttggaa atctgcagcg
      721 cgtaactttt cgcgtttgag ttcaaccagc tttgtaatcc acggatagaa cggcagttca
      781 tccagcagcc cccgtaaagc tgtcaattcc aaatgagagt gaacattgac aaaacccggc
      841 gtgagcgccg tttcgcctaa actgaccgtt tggtatggac catctttggc aacactttct
      901 cgaacaactt cttctggacc aacggctgta atgacaccat tgtgaacagc aaccgcacca
      961 tatggaatcg gcggaactga aaccggaaga acccatcgag cagaatatac aattgtcata
     1021 atgcgttttc taaccaggcg ttcaaaatca aaacatcaat aagagcacgt tgaaggaaaa
     1081 acaggagcca atactttgtt ggcgtgagcc gaaaataata agtggatttg attggttaat
     1141 ctatcaaata ttgtgcgact tgatcagacc aggaaccgtg ggcgaccatc acaccgccag
     1201 ttcactgact gaagatcaga gggttgaagc catttgaatt cagttctttg cctcgctcaa
     1261 ctctcaattt caactcatca ttgagccccg ttatctgctc caggaatttg ccagaaggca
     1321 aggctggcat aggtcacggc tgaggcccgc tcgacttcat gccattgttc atagtggagc
     1381 acctcgccct cacagtcccc aaacacatcg agggcatcga gaaaggtcaa catcgggggg
     1441 aaaccacaaa cattgcgcgc atcctgatcc tgggcaatgt gggtcagcag cgcctgggca
     1501 tcgcgctgtt caactacaga gagcaaggcg gagtcagcct gtgcgacggc ttccagcttt
     1561 tcttcggcat caaacgggtc gccaaatttg cgcccgatat gagccagatc acctccgaca
     1621 agcaaacaac tggattgggg gcgcgattgc agcgtcaccc gaagagcttt gacaaagtct
     1681 tgatattcaa cccgatctgg atttccagcc acgtccgatt cattgagcaa catttgaaaa
     1741 gaagtgcata aaatcggcac catccgcacc cgcccctggg ctaccgcttt tggaaacagg
     1801 tgttgcaaaa aaataacctg aaattcgatg gaatgttcat gccggtgcga ggtgtcatcg
     1861 tggctgatgc tctggccaag ctggcgttca aggtgctcta aaaaagccac atcacactga
     1921 agttgaccaa atggtgtttc ataggctttt cgagaggcca caaacaatcc atttccacca
     1981 taatgagacg ttcccaaaat gatgaatgtc agttgtgcgg ttgggtcatc ttcgagatgt
     2041 gccaggcttt cgcccaggat ccggtaggct ggggcataga ccgccccacc cactcgcaaa
     2101 tcaatgtgtg gagaaatgac ccccacgagg cgtgagtcag ttcgcgcttc aaccggcggg
     2161 ccaccagcca gaatttcatc aagccgaagg ccaagcgcct cggtgtcagc cgggtagctg
     2221 gctccggcat gagcgcaggg tcggactggc gaggcaataa actcagcatc aacttttgac
     2281 tgaaattgtt gaaaccgtgc gctgtcaaac aataaaaagg aatcaaacat ctccaggatt
     2341 tggatgattc gctcttgagg aatagtgaca ccagcctgaa accgaatggc ctgttgcatg
     2401 gtttcaaccg tatgttgtcc atcacaaaac agaagaaata gccccagagc ttcgggaatg
     2461 atcactggct cagtgtaatt ttgtggatca tgggcaataa aaacccgttc cgcttcagct
     2521 tcggctggaa tcaactggac ctggcgcaac cgagggcgct cgggcaacgg aaaatggaga
     2581 tttgagtaca tagaaaagtt gtggaactgt gccagctatg gattgaaggg ggtgaagtac
     2641 cagccgaaat gtgtgtgact cacacgcaag agtgcaggtc gttctattta gcctgaatct
     2701 ggttccagcg caacctcaaa ctcaaggaaa ccagtggaga acatagggtt aaaactctaa
     2761 catcccccgt ttgttttggt aacgatccag aatgcgccgt acatagttac gtgtttccgg
     2821 gtaatcaatc tgggccacat aggtatcggc agtcagttga tttttcccgg cctgggctgt
     2881 tgcggcccgc caccgctctg cttgacttgg cccagcattg tatccagcca gcatcatagg
     2941 caccacaatg gtttcagcct gatagcgctg gcggagcaaa tccagatacc agcagccaat
     3001 agccagatta atttctggat actgtaacaa atcatcttcc gtcagctttg atgcctggga
     3061 acttgataga tttgggaccc gccgggccat ttcccgggca aagttggtgg aagcattttg
     3121 ctcacgccgt ttttgcttcc attcggtaat caccaccggt gtcacctgca tcagaccaac
     3181 ggcgccagcc ccgctccggg ctctggggtt gaaatacgat tcttcataca caatcgcccg
     3241 gaccagtcgc tcttcaagct tatattcggt ggctgttttt gcgataagtt tatcaaaacg
     3301 gtgaatccgc cagtaggtat agccaaaaaa gctggctgac accaccagca caaaaagcgt
     3361 gaatgaaaga agttgtcttt taagggaacg agccataaac ctgcgtgctg agtatgatgc
     3421 ccagtaataa cgccaacggc agtgggatga agacgtcggg cttagggtat tgaacccatt
     3481 ttcttcagcc cagagcttgc gagtcttcag ccccaagccc ggagccctgg ttttcagccc
     3541 ggagcccagg ggttttcagc ctgagaaatc cggcagccaa cgtgctatgc tgccaaaata
     3601 aatcaataaa cctgcagttc ctgagagaaa aactatatgg gcaagattgg gctactggca
     3661 atggatgttg atggaacatt gctcacacct gatggcaaag tgaccgaccg gacacgacag
     3721 gccctggagc aggccgagcg cgctcaccag gtgcatctgg tactggccac cggacgccgg
     3781 tttcacgcgg tacggccagt tgctcaaaat cttggcatcc gcaccccgct cattacccat
     3841 aacggtgcgc tcattaaaaa tctggaaacg aggtcaattt atcgctatca ctggctcgaa
     3901 aatgatattg cccgccgctt aattgagctt ggaaaacaat gtggggctga taccttagcc
     3961 ctggaagacc ccgacgggga tggacggatc ctgaccgaca gcgtttcaga aaacaatgcc
     4021 cccctcaaac gataccttga aatgaatcga gcctatgttc gtccggtgcc aaatttgtta
     4081 cacgaagtca ccggtcccgt gattcaggtt atgttttgcg gtccctgccg acccatggcc
     4141 gaactggcag agattctgct ggcggaaatg tcggaagtaa cccggttact gatgacggcc
     4201 taccctcgca acgatatgac catcctcgac ttgctcaatc cggcggcgtc aaaaggaacc
     4261 gcgcttgaat ttgtggctga attctttgaa attgatcaat cagacgtgat ggccgtgggg
     4321 gataaccaca acgatattga tatgttgcag tatgccggta ttggtgtggt tatgggaaat
     4381 gctgaaccag aactgctgga gatgggattt caccggacag catccaatac tgaagatggg
     4441 ctggcgaaag ccgtggaacg cttcattttg ggactggagt tagggcggaa ggaagaatga
     4501 agaattgaga atgaagaatg aagagaaaat tggtgcct
//
LOCUS       DAOQDO010000175         4538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12799_length_4538_cov_2.939326, whole genome shotgun sequence.
ACCESSION   DAOQDO010000175 DAOQDO010000000
VERSION     DAOQDO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12799_length_4538_cov_2.939326"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2804
                     /locus_tag="PKZ53_03980"
     CDS             <1..2804
                     /locus_tag="PKZ53_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ39625.1"
                     /translation="AISSALAQLPPGMPSPPSMRKVNPADAPILFMVVSSPVLPLTTV
                     NEYAETVIAQQISMVKGVAQVQVFAPQKFAIRVQIDPHELQSRGIGIDEVTSAIRRGN
                     VNLPVGSIYGADQSLTLQANGQLNDSKEYQQLIVAYRNGAPVRLKDVATVIDGVENDK
                     NAGWFNGTRSFTMAVQRQPGTNTIEVVDKIKQLLPGLQEQLPASVRLDILNDRSAQIR
                     ESVHDVQFTLMLTVGLVVMVIFLFLRNVRATIIPSLSLPVSILATFAVMYVLGFSLNN
                     LSLMALTLCVGFVVDDAIVVLENIVRRMDLGEPPRAAALNGAREVGFTVVSMTISLVA
                     VFIPILFMGGILGRLFQEFAVTISLAILFSGLVSLSLTPMLCSRYLRPTAEHNQSWFY
                     RLTEKMFDGVLKVYSSSLRTAMNFGKTTLTLALILVGVTGWIFTQVPKGFIPNDDYGF
                     IFGSTEGIQGISFEEMKKIQEQVVAIVRQDPNVEAVSSNVGAGGRNSTSNSGSMFVKL
                     KPLKNRKVSSEQFINQIRPKLAKVAGVRVFLFNPPAIRIGGISSRSVYQATLQGTDIA
                     QLNAAAQQLERKMRGIKELQDVTSDLENRNPELEVTIDRKQAAAYNLSVEQIQSALND
                     AFGTRQISTIYTESNEYQVIAELKPEFQRDIESVTLLPIRSSTGKLVPLGAVASVHES
                     VGPLIINHVGQLTSVTISFGLRPDISLSQGTALIRQASNGLLPADVTLSFQGTAQVFE
                     ESMKNLNWLLLIAVLVIYLVLGILYESFIHPFTILSGLPSAGMGALLTLLLFKQELNV
                     YGFVGLIMLIGIVKKNAIMMIDFALETQRSSVSSPEEAIYLACVVRFRPIMMTTMAAL
                     MGTLPIALGFGASAQARRSLGLAVVGGLVVSQVLTLYLTPVVYVALERLKVRFGYAES
                     SSTNLFLLKTRSLPTNFNQ"
     gene            3013..4311
                     /locus_tag="PKZ53_03985"
     CDS             3013..4311
                     /locus_tag="PKZ53_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ39626.1"
                     /translation="MKPRIAYLVSQYPAISHTFILREILFLKQQGFEIHTASINSPDR
                     PPERLTLDEKREADCTQYIKTAGVSGALKSLLLTLITHPVGVVRGLVTVARLARFDLK
                     RLVFHLFYFVEALLVGQWMHRHQLAHLHVHFATPASTVGFLTKRIFGVSFSFTVHGPD
                     EFYDVTAYSLREKIEAADFVCCIGFFARSQLMKLSNPAQWSKFEVSPLGIDPELFRPV
                     PFRVTPKPFEIICVGRLVPAKGQHILVSAIHELVQRGGNIHLRFVGDGPDRASLESMV
                     RQLQLESQVQFEGSVNQDRIRELYAASDLFALASFAEGIPVVLMEAMAMEIACVTTCI
                     TGIPELIRSGTDGILVPASDVTALANAIEMLLGNPSLRHQLAQAGRQRVIEKYNLEKN
                     TARLGEIFERRLWLMGNQGSPSTRTTVFSTPDQEIPYKQA"
BASE COUNT         1031 a         1169 c         1136 g         1202 t
ORIGIN      
        1 ccgcgatttc ttcggctctg gcccaactgc cgccggggat gccctcgccg cccagcatgc
       61 ggaaggtaaa tcccgccgat gccccgatcc tgtttatggt ggtgagttca ccggttctcc
      121 cgctgaccac ggtcaatgaa tatgccgaaa ccgtgattgc ccagcaaatt tcaatggtca
      181 agggggtggc gcaggttcag gtttttgcgc cgcagaaatt tgcgattcgg gtccaaattg
      241 atccacatga actccagtcg cgcggcatcg ggattgatga ggtcacatct gccattcgcc
      301 ggggaaacgt caacctgccg gttggttcaa tctacggtgc ggatcaatca ctgacgctgc
      361 aggccaatgg ccagctcaat gattcgaaag agtatcagca actgatcgta gcctatcgca
      421 acggggcgcc ggttcgtctc aaagacgtgg ccacggtgat tgatggggtt gaaaatgaca
      481 aaaacgctgg ctggtttaat ggcactcgca gttttaccat ggcggttcag agacaaccgg
      541 ggacaaacac catcgaagtc gttgataaga ttaaacaact cttgccagga ctccaggaac
      601 agctccccgc ctccgtcagg cttgacatcc tcaatgaccg gtcagcccaa attcgtgaat
      661 cggttcatga tgtccagttt acgctgatgc tgacggttgg actggtggtc atggtgatct
      721 tcctgttttt gcgcaatgtg cgcgcaacga ttatccccag tctttcgctt ccggtctcaa
      781 ttctggcgac gtttgcggtg atgtatgttt tgggatttag tctcaataac ctctcgttga
      841 tggcattgac cctgtgtgtc ggatttgtgg tggatgatgc gattgtggtc ctcgaaaaca
      901 tagttcgccg gatggatttg ggtgaaccac cccgcgctgc cgccctcaac ggggcacgtg
      961 aggtcgggtt taccgtggtt tcgatgacaa tttccctggt cgccgttttt attccaatct
     1021 tgtttatggg tgggattttg gggcgattgt ttcaagagtt tgccgtcacg atcagtctgg
     1081 ccattctgtt ttccgggctc gtttcactct cgctcacccc aatgttatgc agccgatatt
     1141 tacggccaac ggcagaacac aatcaatcct ggttttatcg gttgacggaa aaaatgtttg
     1201 atggcgtcct caaggtttat tccagcagtc tgcgaaccgc catgaacttt ggaaaaacca
     1261 cgctgaccct ggctttgatt ctggtcggcg taaccgggtg gatttttacc caggtgccca
     1321 agggatttat cccaaatgac gactacggct ttatctttgg aagcaccgaa ggcatccagg
     1381 gtatttcctt tgaagaaatg aaaaaaatcc aggaacaggt tgtggccatt gttcggcaag
     1441 acccaaatgt tgaagcggtg tcttcaaacg tgggcgccgg tggccgcaat tcgacgtcaa
     1501 atagtggttc gatgtttgtg aaactcaaac cattgaagaa tcgaaaagtc agttccgaac
     1561 agtttatcaa tcaaattcgc cccaaactgg ccaaagttgc cggcgtccgg gtgtttttat
     1621 ttaacccgcc ggccattcgg atcggtggta tttcgtcccg gagcgtgtat caggcaaccc
     1681 tgcaaggaac tgacattgct caattaaatg ctgctgccca gcaacttgaa cgaaaaatgc
     1741 gtggtatcaa agagttacag gatgtcacaa gcgatttgga gaatcgcaat cctgagcttg
     1801 aagtgaccat tgaccgcaaa caggccgcgg cgtacaacct gtcagtcgaa cagattcaat
     1861 cagccctcaa cgacgcattt ggcacccgcc agatttcaac catttacacc gagtccaacg
     1921 aatatcaggt cattgccgaa ctcaaacctg agtttcagcg agacattgaa tcagtcacct
     1981 tgctcccgat tcgatcaagt actggaaagc tggtgccgct tggcgccgtg gcctcagttc
     2041 atgagagcgt gggcccattg attatcaatc acgtcgggca actgacctcg gtcactatct
     2101 cttttgggtt gcggcccgat atttcactga gccagggaac agccttgatt cgccaggcct
     2161 caaacgggct gttacctgcc gatgttacgc tttcctttca aggcacggcc caggtgtttg
     2221 aagagtcaat gaaaaacctc aactggttgt tgctcattgc cgtgctggtc atttatctgg
     2281 ttttgggcat tttgtatgaa agctttatcc acccatttac gatcctttcc ggactaccgt
     2341 cagccggaat gggggcgctc ttgacactgc tcttgtttaa acaggaactc aatgtgtatg
     2401 ggtttgtggg cctcatcatg ttgattggga ttgtgaagaa aaatgccatt atgatgattg
     2461 attttgccct cgaaacccaa cgttcgtcag tctcttcgcc cgaagaggca atttatctgg
     2521 cctgtgtggt tcgctttcgc ccgattatga tgacgacgat ggcggcgttg atgggaacac
     2581 tgccgattgc gcttgggttt ggcgccagcg cccaggcccg ccgaagtctg ggtctggctg
     2641 tggtcggcgg gctggtcgta tcccaggtcc tgacgttgta tttgacaccc gtggtgtatg
     2701 tggcactcga acggctcaaa gtccgatttg gctatgccga atcaagctca accaacctgt
     2761 tcctcttaaa aaccaggtcc ctcccaacaa attttaacca gtaaggtcac attttttcct
     2821 ttcaatccgt aaggcttggg ctgcctcagt ctcttccagt ttctttctca tgatccggtc
     2881 taccagtttg gcacggcatg tgctctttga attgggcaat tcactctttt gtctcccata
     2941 acgaaagcga cgcaccaccc ccaggtgaag cccccaatca aatcggttgg tttctggtca
     3001 ggatcatctt ttatgaaacc tcgcattgcc taccttgtca gtcagtaccc ggcgatttcg
     3061 catactttta ttttgcggga aatcctgttt ttgaagcagc agggatttga gatccacacg
     3121 gcttcaatca attcgccaga tcgcccacca gaacggctaa cgcttgatga aaaacgtgaa
     3181 gcggactgta cgcaatacat caaaaccgcc ggggtgagcg gggcgctgaa atcattgctt
     3241 ttgaccctga tcacccaccc cgtaggcgtg gttcgtggtc tggtaacggt ggccagactg
     3301 gcccgatttg atttgaaacg actggtcttt cacctttttt actttgtcga agccctgctg
     3361 gttgggcaat ggatgcaccg tcatcaactt gcccacctcc acgttcattt tgcgacgcca
     3421 gcttcaactg tcggattctt gacaaaacgc attttcggag tgtcattttc atttacagtt
     3481 cacggaccag acgaatttta tgatgtcacg gcttattcgc tccgtgaaaa gattgaagcg
     3541 gctgattttg tgtgctgcat tggttttttt gcccgcagtc aattaatgaa actctcaaac
     3601 ccggcacagt ggagtaaatt tgaagtctcc ccgcttggaa ttgaccccga attatttcgc
     3661 ccggttccct ttcgagtcac accgaaacca tttgaaatca tctgtgtagg ccggttagtc
     3721 ccggccaaag gccagcatat tctggtgtca gccattcatg aactggtcca gcgaggaggc
     3781 aacattcacc ttcgctttgt gggtgatgga cctgatcggg ccagcctcga atcaatggtg
     3841 cgccagctcc agttggaatc tcaggtccag tttgaaggtt cggtcaacca ggaccggatc
     3901 cgcgagttat atgcagcctc tgatcttttt gccctggcca gttttgctga aggaatcccg
     3961 gttgtgttga tggaggcaat ggcaatggaa attgcctgtg tgaccacctg cattactggc
     4021 atcccagaat tgatccgctc aggcaccgat ggaattctgg ttccagcttc agatgtcact
     4081 gccctggcca atgccattga aatgttactg ggcaaccctt ccctccgcca ccaactggcc
     4141 caggcaggtc gccagcgggt catcgaaaaa tacaatcttg aaaaaaatac ggcccgcctg
     4201 ggtgaaattt ttgaacggcg attatggctg atgggaaacc agggttcacc gtcaacgcgg
     4261 accaccgtct tttcaacccc tgaccaggaa attccataca aacaggcata accacttcaa
     4321 taactcgaaa tctcccaatc atttcgagcg cggaatacac caaaaaattc ccggtatgga
     4381 gcaaggtcac atgctcggaa aatttatgac aggtatgtat gatgttcttt acgctggctg
     4441 gaattctcat tctgattgta acgcttcctg gaacgattga attgctcctg ctgacagtgg
     4501 gcgccatcgt tggaaagttc acgaccgctc gatatcca
//
LOCUS       DAOQDO010000176         4528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12845_length_4528_cov_5.020344, whole genome shotgun sequence.
ACCESSION   DAOQDO010000176 DAOQDO010000000
VERSION     DAOQDO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12845_length_4528_cov_5.020344"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PKZ53_03990"
     CDS             <1..1124
                     /locus_tag="PKZ53_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39627.1"
                     /translation="DNGHFTWVGGAFRCDVNTPADCVQALGRVRDIATLHVWADDKRT
                     EIQPTNPDEIAARWTETRKFDREFLGLTDEGLVGLTDLDYAAIVCTVGVSEGFSKNNF
                     FEMFLKLLNDDGFHFKWRKSARETAKELYREGKEIERAEFVQMRIDAPDLTPDEVDAS
                     RKATHHTLEQTVALDRYELKGFYMADDAEIPAFIEFDRRGKGRAEIRNLEIALADNAE
                     LQRLTTLESTKLVPDQQNFFILQSLLCTVLAAVGVDCRHFILDPDCRYSTESLKWFVE
                     WVEANRHTLRGVMRIPPPEILASQPVRFISVVLRKMGLKQQRVGKNKKGQYRLDSDQF
                     EQIQAILERRGTFPTQIPASGDTSHLIIHEDSQCPRRRH"
     gene            complement(1172..1714)
                     /locus_tag="PKZ53_03995"
     CDS             complement(1172..1714)
                     /locus_tag="PKZ53_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNJ39628.1"
                     /translation="MLITYYVGDVPERGQGVALKFAYHPDLVATVKHSLREARVAYYR
                     RTRQPADQIGGWVPGIRVWFCPVELWAETARRLEGLGHELEAQFDERFFFTLPWNEIG
                     NHRTRRQSLPNSTHTTASDWELFGLTPAASPAELKQAYRDLVEVWHPDRFAHNPRLQA
                     KAEAMTKQINATYQRLQVAA"
     gene            complement(2102..3610)
                     /locus_tag="PKZ53_04000"
     CDS             complement(2102..3610)
                     /locus_tag="PKZ53_04000"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ39629.1"
                     /translation="MSKKLSLRWGVWILIALSVGSTYLNPKITGTFKQISGDPEFERE
                     KKEFLEINLFWWKYLEQLLPASDPKGVRLSCPVNSVSFFPDGSSIVTCDHKLESVTLN
                     TLTGEQSKTFGRNQVLEQIGFSKDGNWAALCTATGELTIRNLNTGEEKSISELDGALV
                     KKLALSPDGQWLAVSLNEKQIAILNLHSNGRVLKAEGHTSPISRIEFTQDGGQLISSD
                     YDSSLCIWNLKLNPITLDKIRVPFSIFSFSISPNGDQVAVCGKNESVHLISLHTGKLE
                     KVLVGHTESVNRVVYSPQGQWLATGSLLEQTIKLWSTSTWEEALTFRGHFNNVFDVSF
                     SPDGEWLVSCGSDQVVGWWKVPQNDLELDQYVKALRVVDFVRSVRFGHQKEVEQTIAR
                     YPDIVHFVDTNGVSPLLWAIKEGKTQTASVLLSKKANPNVQEIQSGMTALHYAAKVGD
                     LESVQLLIEYKASIQSKDQFNLTPLEYAKKMKRRNVVRFLQTVMRKNIEKPK"
     gene            3729..3911
                     /locus_tag="PKZ53_04005"
     CDS             3729..3911
                     /locus_tag="PKZ53_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39630.1"
                     /translation="MVFAVEMNITSKTEVGSKKFTGLGFAQKAGVHGKYQRAETSWAT
                     MKQPSSEFGYFLVFAD"
     gene            complement(3887..>4528)
                     /locus_tag="PKZ53_04010"
     CDS             complement(3887..>4528)
                     /locus_tag="PKZ53_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ39631.1"
                     /translation="LLLDAGADVNARTSDGDTVLIRSASYASREVIGALLNAGANVNL
                     QNTTGDTALHSAAFNGRSDILQMLLGAGATVDTSDIGGYTALIWAASNNHGEALRVLL
                     KFGANMNAANNDGYTGLMLATVHGHHETVKVLIEAGADLNMQTKEKSYSALHIAALNG
                     HELVVETLLTAGADTMLKTRNGETALQIAERRNAERCGFAGIVRLLQSANTRK"
BASE COUNT         1137 a         1110 c         1084 g         1197 t
ORIGIN      
        1 ttgataatgg gcatttcaca tgggtgggtg gtgcgtttcg ctgcgatgtc aatacaccgg
       61 cggattgcgt tcaggcactg gggcgggtgc gagatattgc gacactccac gtttgggcgg
      121 atgacaaacg aactgagatt cagccaacta atccagatga aatcgcagcc cggtggaccg
      181 aaactcgaaa attcgaccgt gagtttttgg gcttgactga tgaagggctg gtcggactca
      241 cagacctgga ctatgccgcg attgtgtgca ccgtgggagt gagcgagggg ttcagtaaaa
      301 ataacttctt tgaaatgttt ttgaaactac tcaacgatga tgggttccat tttaaatggc
      361 ggaagtcagc cagagagacg gcaaaggaac tgtatcggga agggaaggaa attgagcggg
      421 cggaattcgt gcaaatgcgg attgatgcgc cggatctgac acctgatgaa gtagacgcca
      481 gccgcaaggc gacacaccac accctggagc aaaccgttgc gctggatagg tacgagttga
      541 agggatttta catggctgat gatgccgaaa ttcctgcttt tatcgagttt gaccgacgcg
      601 ggaaagggcg ggcggagatc cgcaacctgg aaattgcact ggctgataat gccgagttgc
      661 agcgactgac gacgctcgaa agcacaaagc tggttccaga ccagcagaat ttctttattt
      721 tgcaaagcct actctgcact gttttggcag cggtcggagt ggactgtagg catttcatcc
      781 ttgatccaga ctgcagatac tcaactgaat cactcaaatg gtttgtcgag tgggtggaag
      841 ccaaccggca taccttgcgt ggagtgatgc ggataccacc gccagagata ctggccagtc
      901 aacctgtgcg gttcatttcc gtggtgctcc ggaaaatggg gctcaagcag cagcgggttg
      961 gaaaaaataa aaagggccaa tatcgccttg attcggatca atttgaacaa atacaagcta
     1021 ttttggagcg gcgggggacg tttccgactc aaattccagc gagtggggac acttcacacc
     1081 ttataataca tgaagactca cagtgtcccc gaagaagaca ttaaccaggt taggtgaaaa
     1141 cttcgaaaac cggagagatc agtttcaact gtcaggcggc gacttgtagt cgctgatagg
     1201 tagcattgat ctgtttggtc atggcttcag ccttggcctg caatcgggga ttatgggcaa
     1261 agcggtccgg atgccagacc tcgaccagat cacgataggc ttgcttgagt tcggcggggc
     1321 tggcggcggg agttaatcca aacaattccc aatcggaagc cgtggtgtgg gtcgaattcg
     1381 gcagtgactg acggcgggtc cggtgattcc cgatttcatt ccaggggagc gtgaaaaaga
     1441 aacgttcgtc aaactgggct tccagttcgt ggcccagacc ttccaaccgc cgggcggttt
     1501 cggcccacag ttcaactggg caaaaccaga cgcgaatgcc tggcacccag ccaccgatct
     1561 gatcggctgg ttggcgggtt cggcggtagt aggcgacccg agcttcccga agcgagtgtt
     1621 tgaccgtggc caccagatcc ggatggtagg caaatttgag tgccacgccc tggccacgct
     1681 ccggcacgtc tccgacataa taggtgatca gcatggttgc tttcatcctc ctcttcaaac
     1741 aaagtaccca catgccttgt tcggaaaacc ggtaacgaca gtgtacccat ctctcgccca
     1801 ttgcgacaag gaacatgccg ttcaagcccc aacaaagaca aaatcaggta aattgcatcc
     1861 tgtcttcact gaaagacacc catggccctg attttttcca gtatttctcc ggtcaggttc
     1921 gggaaccttg cggaatggct acgtctgaag cgacacctct gggttgggtg atgaagatca
     1981 tcgtcaggtt gattccagag agcaccggcg ctggagttca gtcaatcggg attggggtta
     2041 atgacacccg catgacctgg ttgttgcatc agcatacctc tcaagacaaa actctgaacg
     2101 atcattttgg tttttcaata ttcttcctca tgactgtttg gagaaatctt acaacatttc
     2161 tccgtttcat cttctttgca tactcaagtg gcgtcaggtt gaactggtct ttactttgta
     2221 tagaagcttt atactcaatc aataattgca ctgattccag gtcaccaact ttcgcagcat
     2281 aatgcaaagc tgtcatcccc gattggattt cttgcacatt cgggttggcc tttttggaaa
     2341 gtagcacact ggccgtttgg gtcttacctt ctttgatggc ccacaagaga ggtgacactc
     2401 cattggtatc cacaaagtga acgatatctg gatacctggc aatcgtctgc tcaacttctt
     2461 tttgatggcc aaacctgaca cttcgaacaa agtcaaccac ccgtagcgcc ttcacatact
     2521 ggtcgagttc caagtcattt tgtggaacct tccaccaccc aaccacctgg tccgaaccgc
     2581 atgagaccag ccattccccg tcaggtgaaa agctgacatc gaatacattg ttgaagtgtc
     2641 ctcgaaaagt cagggcttct tcccaggtac ttgttgacca taacttgatc gtttgctcca
     2701 ataaacttcc tgtagccaac cattgacctt gaggagaata tacaacccga ttgactgatt
     2761 cagtatgccc taccaacact ttttccagtt ttccggtgtg caaactaatc agatgaaccg
     2821 attcattctt cccgcagaca gcaacctgat cgccattggg agaaatcgag aaagaaaaaa
     2881 ttgaaaacgg aactctgatc ttgtcaagcg ttattggatt caactttaag ttccagatac
     2941 agagggagct gtcgtaatcg cttgaaatta attgtccgcc atcctgggta aactcaatcc
     3001 ttgaaattgg agacgtgtgg ccctccgctt ttaaaacccg cccgttgctg tgaaggttta
     3061 agatggctat ttgcttttca ttcagactca ctgccaacca ctgtccatca ggtgacaacg
     3121 ccaatttctt gaccaaagct ccatccagtt cagaaattga tttttcctct ccagtgttga
     3181 gatttctgat cgttagctcc ccagtggctg tgcaaagggc tgcccaattc ccatcttttg
     3241 agaatccgat ttgttcaagt acctgatttc gaccaaatgt tttggattgt tcaccagtca
     3301 acgtattcaa ggtcaccgat tccaatttat gatcacaggt gacaattgag ctgccatctg
     3361 gaaaaaagct taccgagttg actgggcatg ataggcggac tccctttgga tctgaagccg
     3421 ggagcaattg ttctaaatac ttccaccaaa acaaattgat ctctaagaat tctttctttt
     3481 ccctttcaaa ttcgggatct ccactaattt gtttgaaggt gccagttatt tttgggttta
     3541 gatatgtaga tccaactgag agcgctatca gtatccaaac tccccacctc agtgatagtt
     3601 ttttcgacat catttcctct acttgccagg agaatgcctt cctgtcactt ctttgttgtc
     3661 ttatcctatt tgatcgcagt ggtttacatt ctaatccaaa gtaactgtat acacggaacc
     3721 tcggttcttt ggtatttgcc gtggaaatga atataacctc aaaaacagag gttggttcta
     3781 aaaaatttac aggcttaggt tttgctcaaa aagctggggt tcacggcaaa taccaaagag
     3841 ccgaaacttc ttgggccaca atgaaacaac catcaagcga gtttggttac tttcttgtat
     3901 ttgcagactg aagcaatcgg acaattccag caaacccgca ccgctctgca ttccgtcgtt
     3961 cggcaatctg aagtgccgtt tccccattcc tggttttgag cattgtatct gccccggctg
     4021 tcaacaaggt ttcaacaacc agttcgtgtc cgttgagtgc cgcgatatgg agtgcactat
     4081 agcttttttc tttcgtttgc atattcaaat ccgccccagc ttctatcaac actttcaccg
     4141 tttcatggtg tccgtgaact gtcgccagca tcagtccggt atatccatcg ttattggccg
     4201 cattcatatt tgcgccaaat ttgagaagaa ctctcaaagc ctcaccgtgg ttgttgctgg
     4261 cggcccagat aagtgctgta taccctccga tatctgaggt gtccaccgtt gctccggcac
     4321 caagcaacat ttggagaata tcgctgcggc cattaaatgc agcggaatgg agtgctgtat
     4381 caccagtagt gttttgtaaa ttgacatttg ccccggcgtt caacaatgcg cctatcactt
     4441 cacgacttgc gtaactggca cttcttatca atactgtatc tccatcagag gtacgtgcat
     4501 tgacatctgc tccggcatca agcagcaa
//
LOCUS       DAOQDO010000177         4520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12894_length_4520_cov_2.684211, whole genome shotgun sequence.
ACCESSION   DAOQDO010000177 DAOQDO010000000
VERSION     DAOQDO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12894_length_4520_cov_2.684211"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4520
                     /locus_tag="PKZ53_04015"
     CDS             <1..>4520
                     /locus_tag="PKZ53_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HNJ39632.1"
                     /translation="ERECRETVEAWMLRSLVIPRGILQAVWSDGAWRAPLENTVVMPV
                     DLDGKVSPDQAGLFRGVDESKGIGVVNLDGETLWIDTPLVVIPHPILLPELNDFRDLL
                     TDLGIKQGISQVFREIWPKSTTLSAATTNLNTYSDGHFEQLNHALGRCRKLGYRVRGG
                     WACGPVWEGGKLVEARYWIGAEYPESETYTGELIWVDAKERTLKLADVGPVAFSEGNR
                     MASLIYAARKVEKKEGEASTEETTETETPTAAPVPVNIAVGSSISVSTTDTLRAGGLV
                     LVESQVSDKEPVDRVSARTYCHPAMPEETVVRLSADVLSRGDDRVMEVLGFDPPDIKP
                     ELALQRRRAIGFPGWALIHDPDRASFALDVVKEMKKHGRLAKSKPGHAKDGFDQIGER
                     LGRSVPHYLPSYYEEVGRIYIELGNQTYAAQAFNKAREAERVHALKIDEAQRQASFLD
                     FALAGALTVKALSQYAKDLLGGFTPEIAYKTFRDLVVKRTLGGLPPWGSVGKDIRPLM
                     TAAGLNVEAEERVLLEEFLDSPVMAKAPVEFWTAYRKPLLALAKTAPKIRGILLNLFP
                     KGNDEVKFHQQWLELLEECGALEAVIAPAETVPEECRPVGGAAAWFSRELSHTFSGYR
                     SRLPFELFGLLRRMSPRLIAEGQPITLDTTKYYQADIDLDLLEQALELGVPINDPQPK
                     SSIDLAKWLHPPDKSSEWRRDPIHTAAEPRFRPLLINGVGRVINNSEFHQAAQGKKGL
                     TEARRLWYEAQATELETAFLGDFLHEVSVLEDAITPAVLEEFPEIASRLKAIDPAVIV
                     SRTLQAGMLDEYGWPDLEAAVEELTTGVKDPVVRLMGAFPYLIVSDTHKAIVVGPKGR
                     VKTLQLNLPAKAYLMTCRYVDGELIVAYRDSSYHTKAFWTSNPMEQIDLGYMGNYQIG
                     TLALPVPGGGTTEGQATLRVGDTKIDFGYGSITDKVLSDGQHFWRIEHKDGKPFREFD
                     PATGEGGRWSLPKFYEEFSTGDKPIFAYLSHFLAPLPEGLEQTPLGTKNGLTGFRGRC
                     PKIDNIHQSEAAAQGYELEGIDGRRFEGNLRSVTDYFPQGLVQFPGDGLFRPVLATYN
                     MISLWDPAGKHKLVSIEFSKLNPEEARGTTAVFPLVWWHFLKVRDQAGSEALRRISAE
                     ACRQLLQTARAECPKPDDDDDDDVDFDLSKLPKTLETLQQVLPEVTHPRLQRGILGIV
                     AQAAQISQQYSKVLGESDSDEPSPNLSASLPTFSNLVAGAFLPLVETGWGRWSSEEQN
                     LGRQFVETDQFFREELDPAATLERKAAHARVQWETLIGRISAIALKAAVFEMDADLRE
                     KLLEFLEFWSKSVFAAPDTKIRFIAGQKGTPETVGAYRYDDPREGERLVTHAGNRYLL
                     KTVRNYHTQRFNFSGIEYAVSGEFQLLPETTIERSQELESGRLSSENLHTLITAVKER
                     GPVAWNPAIAEDLANRTGLSKAAAALLWLGCPKFEDYSDNFLPKDLRTRLGLKVAEAV
                     AADRRIHHGAAR"
BASE COUNT         1060 a         1146 c         1286 g         1028 t
ORIGIN      
        1 gagcgcgaat gccgcgaaac cgtcgaagcc tggatgctcc ggtcgctggt gattccacgc
       61 gggattctgc aggcagtttg gtcagacggg gcctggcggg cgccgcttga aaacacggtt
      121 gtgatgccgg tggaccttga tggaaaggta tcgccagacc aggccggact gtttcggggc
      181 gtggatgaat ccaaagggat tggcgtcgta aacctggatg gcgaaacact ctggattgac
      241 acgccgctgg tcgtcatccc gcatccgatt ttgctgcctg aactgaatga ttttcgcgat
      301 ttgttgactg atttgggcat caaacagggt atttcacagg tctttcgtga aatctggccc
      361 aaatccacca cgctgtcagc cgcaaccacc aatctcaaca cttattcaga tggacatttc
      421 gaacaactca accatgcgct gggtcgctgt cgcaaactcg gctatcgagt gcgcggcggt
      481 tgggcctgtg gcccggtgtg ggagggtgga aaactggttg aggcccggta ctggattggg
      541 gctgagtatc cagaatctga gacttacact ggtgaattga tctgggtgga tgccaaagaa
      601 cgcacgctga aactggctga tgtcggcccg gttgcatttt cagaaggcaa tcgcatggcc
      661 agcctgattt atgccgctcg caaagttgag aaaaaagaag gtgaggccag taccgaggaa
      721 acgactgaga ccgaaacacc aactgcggca ccggtgccgg tcaatatcgc ggttggaagc
      781 tcaatttcgg tttccacgac cgacaccctg cgggctggcg ggctggttct ggtggaaagc
      841 caggtgtctg acaaagagcc ggttgatcgc gtcagcgccc ggacctattg tcatccggcg
      901 atgcccgaag aaaccgtggt gcgtctctcg gctgatgttt tatcccgagg tgatgaccgg
      961 gtgatggaag tccttgggtt tgacccgcca gacatcaagc ccgaactggc cttgcaacgc
     1021 cgccgtgcga ttggatttcc tggctgggcg ctcattcatg acccggatcg ggcttcgttt
     1081 gcactcgatg tggtcaagga aatgaaaaaa cacggacgac tcgccaaatc caaacccgga
     1141 cacgccaaag atgggtttga tcaaatcgga gagcgccttg gacgttcagt gccgcattac
     1201 ctgccgtcgt attatgaaga agttggacgg atttacattg aacttggcaa tcaaacctat
     1261 gctgctcagg catttaacaa ggcccgcgaa gccgagcgcg tccacgcctt gaaaattgat
     1321 gaagcgcaac gccaggcgtc attccttgat tttgccctgg ccggggcgct gacggtgaag
     1381 gcgctttcac aatatgccaa agatttgctt ggcgggttta cgccggaaat tgcctacaaa
     1441 acttttcggg atctggtggt caaacgaacg cttggcggac tgccgccctg gggaagcgtt
     1501 ggaaaggata ttcgaccgct gatgaccgcc gccgggttga atgttgaggc cgaagagcgc
     1561 gttctgctgg aagaatttct ggactcgccc gtaatggcca aagcgccggt tgagttttgg
     1621 accgcctatc gaaaaccgct ccttgctctg gctaaaaccg ctcccaaaat ccgaggcatc
     1681 ctgctcaatc tgtttccaaa aggaaacgat gaggtgaaat ttcatcaaca gtggcttgaa
     1741 ctgcttgaag aatgcggcgc ccttgaggct gtgattgccc cagcggagac agtccctgaa
     1801 gaatgtcgtc cggttggggg cgctgccgcc tggtttagtc gcgaactcag ccacaccttt
     1861 tcgggatacc ggagccggct gccatttgaa ttgtttggtt tgttacgtcg gatgtcccca
     1921 cgcctcattg ctgaagggca acccatcacc ttagacacca caaaatacta tcaagctgat
     1981 attgaccttg atttgctgga gcaggccctg gagcttgggg tacccatcaa cgatccgcaa
     2041 ccgaagtctt cgattgacct tgccaaatgg ttgcatccac ctgataagtc aagcgaatgg
     2101 aggcgcgacc caattcatac ggctgccgag ccacggttca ggccactgtt aattaacggg
     2161 gtgggcagag tcattaacaa ttctgaattt catcaggccg cgcagggcaa aaaaggtctc
     2221 accgaagccc gtcggttgtg gtatgaggcc caggccactg agcttgaaac tgcttttctt
     2281 ggagatttct tacacgaagt cagtgtgctt gaagacgcga ttacccccgc agtgcttgaa
     2341 gaattccccg aaattgcgag ccgtctcaaa gcaattgatc cagcggtgat tgtgtcacga
     2401 accttgcaag ctggcatgct ggacgaatat gggtggcctg acctggaagc ggcggttgaa
     2461 gaactcacca ccggagtcaa agatcccgtc gtccggttga tgggggcttt cccgtatctg
     2521 attgtcagtg atactcacaa ggcaattgtg gtggggccaa aaggtcgggt gaagacactg
     2581 cagttgaatt tacctgcaaa ggcttatttg atgacgtgtc gctatgttga tggtgaattg
     2641 atcgttgcct atcgtgattc gagctatcac accaaagcct tctggaccag taaccccatg
     2701 gaacaaattg acctggggta tatggggaat taccagattg gaacgctggc tttgccagtt
     2761 ccaggtggtg gcactaccga aggtcaggca acattgcgcg tgggcgacac caaaattgat
     2821 tttggttatg gctctatcac ggacaaggtc ctgtcggatg ggcagcattt ctggcgcatc
     2881 gagcacaaag acgggaagcc atttcgcgaa tttgacccgg ccactggcga aggtgggcgc
     2941 tggtcactgc caaagtttta tgaggaattc agcaccggag acaaaccaat ctttgcctat
     3001 ctcagccatt tcctggcccc gttgcccgaa ggattagagc aaacaccgct gggcaccaaa
     3061 aatgggctca ccgggtttcg gggccgctgc ccgaaaattg acaatatcca ccagagtgaa
     3121 gccgcggctc aagggtatga gctggaaggc attgatggtc ggcgctttga aggcaatctc
     3181 agatctgtga ccgattactt tccgcaagga ctggttcagt ttccgggtga tggtctgttt
     3241 cggccagtac ttgcaaccta caacatgatc agtctgtggg atccagccgg gaaacacaaa
     3301 ctggtctcaa ttgaattctc aaaactcaat ccagaagaag ctcgtggcac aacggcggtg
     3361 ttcccactgg tttggtggca ttttctcaaa gttcgtgacc aggcgggttc tgaagccttg
     3421 cggcgaattt cggctgaagc ctgccggcaa ttgttacaaa cagcccgcgc cgaatgccca
     3481 aaacctgatg acgacgatga tgatgatgtg gactttgacc tgtcaaagct gccgaagacg
     3541 cttgaaacac tccagcaggt tttaccagag gtgacacacc cccggttgca gcgtggaatt
     3601 ttggggattg tggcgcaggc ggcgcaaatt tcgcagcaat attcaaaggt gctgggtgaa
     3661 tcggactcag atgagccgtc cccaaatcta tcagcgagcc tgccgacatt tagcaacctg
     3721 gtcgccgggg cgtttctgcc actggttgaa accgggtggg ggcgctggtc atcggaagag
     3781 caaaatctgg ggcgtcagtt tgtggaaacc gatcagtttt tccgtgagga actcgatcca
     3841 gccgcaacct tagaacgcaa agccgctcat gcccgagtcc agtgggaaac actcattggg
     3901 cggatttcag ccattgctct caaagcggcg gtttttgaaa tggatgctga cttacgcgaa
     3961 aagctgcttg agtttttgga gttctggtca aaatcagtgt ttgcggcacc agacacaaaa
     4021 atccggttta ttgccgggca gaaaggaacc cccgagacag tcggcgccta ccgatatgat
     4081 gatccgagag agggggaacg cctggttacg catgccggca atcggtatct gttgaaaacg
     4141 gtgcgcaatt accataccca gcgatttaat ttttccggta ttgaatatgc agtcagcggc
     4201 gaatttcaac tcttgcccga aaccacgatt gaacgatctc aggaactcga gagcggtcgc
     4261 ctgagttcag agaaccttca caccctgatt actgccgtga aggaacgtgg cccggttgcc
     4321 tggaatccag cgattgcgga agacctcgcc aatcgaaccg ggctttcaaa agccgccgct
     4381 gctttgctgt ggttaggctg tccaaaattt gaagattact cagataattt cctgcccaaa
     4441 gatttgcgga cgcgccttgg tctcaaagtt gccgaagcgg ttgctgcaga tcgccgaatt
     4501 caccatggag cagcccgatt
//
LOCUS       DAOQDO010000178         4517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12901_length_4517_cov_3.060287, whole genome shotgun sequence.
ACCESSION   DAOQDO010000178 DAOQDO010000000
VERSION     DAOQDO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12901_length_4517_cov_3.060287"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1105)
                     /locus_tag="PKZ53_04020"
     CDS             complement(44..1105)
                     /locus_tag="PKZ53_04020"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNJ39633.1"
                     /translation="MRRLKQDFSHFSGCLIGGAIGDALGWPVEFHKLPQIHERFGPEG
                     ITDFPEETGQVTDDTQMTLFTAEGILRYINAEQAEYPTDLVQELYVAYLRWLVTQGSL
                     VSGSADTSVVRDGWLFSIPDLYHRRAPGMTCLSALASGKCGTINRPLNNSKGCGGVMR
                     VAPCGLYVRGVQAYELGCRAAAITHSHPLGYVTAGAFALMIAELIDGASLDAAIGRGI
                     EQLYRDPMLVSSECAQLLERAVHFAQELPGDLNRLRSLGEGWVAEECLAIAVYAALSF
                     PDNFEKALIFAVNHDGDSDSTGAVTGNLLGALLGIDSIPEPWVRRVEFKEVLAQVATD
                     LLLPADDQAGRSERYPISK"
     gene            complement(1201..2100)
                     /locus_tag="PKZ53_04025"
     CDS             complement(1201..2100)
                     /locus_tag="PKZ53_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39634.1"
                     /translation="MVFSRKFCLLTLMLILTGSLPVVSLTAQKKNQKSVLFCLERYED
                     GKTAMEPIVVLSSGKFLAPPTGIESTEDLAAQDVESVKFLKNYYRKGQSYRVVFGTAE
                     DGTVTVMKPQESGCVSLSAQVSVASSVKLGGRVYALATDHPTLGVGKKRSRRAPTPEE
                     RSAAIAIARDFLRGKKVPAALMKLMQVGNLTAVDLNGDGKDELVGNFEIDQSDTVLEA
                     RSVFFILSPVGVEFKIAFSEYYQGSEEQYRRSIFIDHLDLDGDDISEVIIRTTYYESF
                     SYSIIKNKGGKWVNVYTGGESGC"
     gene            complement(2379..2981)
                     /locus_tag="PKZ53_04030"
     CDS             complement(2379..2981)
                     /locus_tag="PKZ53_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39635.1"
                     /translation="MAHCISKYGLAGWASPIVGRVLVGVMVVAGYWITTSPVLAQAPP
                     PKPSSAEFLKEINRDVWIPFSKAYATGDAEAYLNLHSKDLIRGQGNSREVLNLTQYGT
                     GIRNFFKKVKTDGGKLGISFRFQERIANAEFGSERGFFEFSFTNAKGETRKAYGKFHV
                     FLRKESGTWKILVDYDSNEQNTITEATFNAGTAVDDVTKY"
     gene            3217..3822
                     /locus_tag="PKZ53_04035"
     CDS             3217..3822
                     /locus_tag="PKZ53_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39636.1"
                     /translation="MVTKINFSVPTVLLMFLALIGWIGVPGHPVSGQAPKTVLDYYLQ
                     LPNRYLFSGGDRAARMKLIKIQDVKNGYLRLEGDWEGFSEIALFRQPNGTPLIVVTNA
                     ECGPGCTQSTYFLEFRDGQWVNRQAELFPLTRQQVVAEYRKSGLKTIEDFGDDLPYLI
                     MLPRAGRVIKVIVQPEFTETEQTIMAFELKDGVFQRVLRTK"
     gene            complement(4050..>4517)
                     /locus_tag="PKZ53_04040"
     CDS             complement(4050..>4517)
                     /locus_tag="PKZ53_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39637.1"
                     /translation="LTAAGIIIGTPTYMSPERLEGSPYDGRSDVYSVGIMMYEMVCGC
                     LPFQPSSRGVLDVILRQIKEMPSPPREINPDLPESVERIILRALEKNPLVRPSITELE
                     QAFLAALEGLPEAILNAGPVHVVQPWNIGLEVTIEDSLSNMTTDIVPPSKETL"
BASE COUNT         1174 a         1179 c         1020 g         1144 t
ORIGIN      
        1 gtccccctgt catcctgttc cctgatcctg atgccttcat ccttcacttt gaaatcggat
       61 atcgttcgga tcttcccgcc tgatcgtcag caggtaacaa caagtccgtc gccacctggg
      121 ccagaacctc tttaaattcc acgcgtcgta cccagggttc gggaatgcta tcaatcccta
      181 acaatgcccc aagcaggttc ccggttacag caccagtact atcgctgtct ccgtcgtggt
      241 tcaccgcaaa aatcaacgct ttttcaaaat tatctgggaa tgaaagagcg gcatacacgg
      301 caatcgccag acattcttcg gccacccagc cttcacccaa actccgcaac cggttcaggt
      361 ctccgggaag ctcttgggca aagtgaacag ctcgttcaag gagctgagca cattctgagc
      421 tgaccagcat tgggtcacga tagagttgct caattccacg gccaatagca gcgtcaagtg
      481 atgcaccatc aatgagctcg gcaatcatca gcgcaaatgc cccagccgtg acatatccca
      541 atggatgact atgcgtaatg gctgcagccc ggcagccaag ctcgtatgcc tgaaccccac
      601 gaacatagag accacagggg gcgacacgca tcaccccacc acatcctttg ctgttattta
      661 atggacgatt gatggtgcca catttcccac tcgccagggc cgaaagacag gtcatccctg
      721 gggcgcgccg gtgatacaga tcaggaatgg aaaacaacca tccatcgcga acaactgatg
      781 tatcagccga tccactgacc agacttccct gggtgaccag ccatcgcagg taggcaacat
      841 agagttcctg aaccagatcg gtcgggtact cagcctgttc agcattgatg taccgcaaaa
      901 tcccttctgc cgtaaacagg gtcatctgcg tgtcatcagt cacctggcca gtttcttcag
      961 gaaaatcagt gatgccttcc ggtccaaacc gctcgtggat ctgaggaagc ttgtgaaatt
     1021 cgactggcca ccccagggca tctccgatgg cgccaccaat gagacacccg gaaaaatgac
     1081 taaaatcctg ttttaatcgt ctcatcacgc ctccttttta aagacagggc tgaagatctc
     1141 gggcttgggg ctgaagaatt tgggctgaaa atatggttac cctgtcacgc tgtcacctgt
     1201 tcaacaccca ctttcgccgc cggtgtagac attgacccac ttcccgcctt tatttttgat
     1261 gatgctgtaa gaaaagcttt cataatacgt tgttcggata atgacttcgc tgatgtcatc
     1321 gccatcaagg tccaggtggt caataaaaat tgaacggcga tattgctctt cgcttccttg
     1381 atagtactcg gaaaaagcga tcttaaactc aactccgacc gggctgagaa tgaagaatac
     1441 tgaacgtgcc tccagaacgg tgtctgactg gtcaatttcg aagttcccca ccagttcgtc
     1501 ttttccatcg ccatttaaat caacggcggt gagatttcca acctgcatca atttcatcag
     1561 cgcggcaggc actttcttac ctcgtagaaa atctctggca atagcaatgg cagcagagcg
     1621 ttcttcaggg gttggggccc gccgtgaacg tttttttcca acgccaaggg ttggatggtc
     1681 agtagccagg gcgtaaactc ggcctccgag cttcacgctg ctggcaactg aaacctgcgc
     1741 agataaggac acacaccctg actcctgcgg tttcatcacg gtcacggttc cgtcttcggc
     1801 agttccaaac accacccggt agctttggcc cttacgatag taatttttga gaaatttgac
     1861 gctttcaacg tcctgagctg ccaggtcttc agtgctttca ataccggtcg gaggcgcaag
     1921 gaattttcca ctcgacagaa caactatcgg ctccatcgct gttttcccat cttcatatct
     1981 ctcaagacag aacaaaaccg atttctggtt ttttttctgg gcagtcaggg acacgaccgg
     2041 gagtgatcca gtcaaaatga gcatcaatgt cagaagacaa aactttcggg aaaagaccat
     2101 aacaacctca gttggaaaga gatgaaatga agcgctccca actatcctgc ctttcaaatg
     2161 gaattgcaat attgaaaatg ggttaccagc ccagcggata cgagctaaac tattgattca
     2221 tagacgattg ctgtcttgat actcaagttt gacttctctg tttttcccgt tccggaaatt
     2281 ttccggaacg gggaaattat atcaaccagc aatgaaaaag ccttcgctga aatgagagca
     2341 aaggctggca caaagaaatc cagattcagg tcaggcaatc aatattttgt tacatcatcc
     2401 accgccgtcc ccgcattgaa ggtcgcttcg gtgatcgtgt tttgttcgtt tgaatcgtaa
     2461 tccaccagaa ttttccaggt accgctttcc ttgcgcagga agacatgaaa cttgccatat
     2521 gccttccggg tttcaccttt ggcattggta aagctgaact caaagaaccc tcgctcagat
     2581 ccaaattcag cgttggcaat ccgctcctga aaccggaagc tgatccccag ttttccaccg
     2641 tcggttttga ccttcttgaa aaagttgcga atgccagtgc catattgcgt caaattcaag
     2701 acttcccggc tgtttccctg gcctcgaatc agatctttgg aatgtaaatt caggtaggct
     2761 tctgcatcac ccgtggcgta cgcttttgaa aacggaatcc agacatcacg gttgatttct
     2821 tttaaaaact cagcgctgga aggtttcggg ggcggggctt gagccagaac cggcgaggtt
     2881 gttatccagt atccagccac aaccatcacg ccaaccaaaa cccgaccaac gattggggat
     2941 gcccaacctg ccaatccata cttcgagata caatgagcca tagtttcttc attctcctgt
     3001 tgatgaatgt cagcgattga actacaaatt cactgaaaag aaaacttcaa agtgaaaaga
     3061 attgaatttc tttgaaagct aaatttttca acaatttaaa ctgaaattgg tctcaaaaat
     3121 tcccgttccg gaaattttcc ggaacgggga atcaccagca agtattttca aaaagtggtt
     3181 ttaaaactca ctattttccc tcggagtcat cagattatgg taaccaaaat aaacttttca
     3241 gttccaaccg ttctgcttat gttcctggcc ctcatcggtt ggattggggt tcccggacac
     3301 ccggtttcag gtcaagcccc aaaaacagtg ctcgactatt accttcagct tccaaaccgg
     3361 tatctcttct caggcggaga ccgggctgcc cgaatgaaac tcattaaaat tcaggatgtg
     3421 aaaaacggct atttacgact tgaaggtgac tgggaaggat tttctgaaat tgctttgttt
     3481 cgtcagccaa atggcacacc gctgatcgtt gtgaccaatg ccgaatgtgg ccctggatgc
     3541 acgcagtcaa cgtattttct ggaatttcgc gacgggcaat gggtgaatcg ccaggcggaa
     3601 ctgtttccgc tcacccgcca gcaagttgtg gctgaatacc gaaaatcagg actgaagacg
     3661 attgaagatt tcggagatga cctcccttat ctgattatgc taccccgtgc tggtcgggtc
     3721 atcaaagtaa ttgtccagcc tgaattcact gaaacagagc aaaccatcat ggcctttgaa
     3781 ttaaaggatg gcgtttttca gcgagttttg aggacaaagt gacctggtga accgcatttc
     3841 tggcggcgag gtcgcttgat tttcttttca aaatttaaac ctggaaaatg cccgtccagg
     3901 aaaagagctg gcatctatct cattgaaaaa tagatattta accagttttt cattcttcat
     3961 tcttcactct ttattcaaaa aaatccccgt tccggaaatt ttccggaacg gggatttttg
     4021 ccagcattga gcacctggac cctggcgctc taaagcgttt ctttcgacgg tggcacaatg
     4081 tcagtggtca tattactgag gctgtcttca atcgtgacct ccagcccaat gttccagggt
     4141 tggaccacgt gcactgggcc agcattcaat attgcctcgg gaagtccctc aagggcggcc
     4201 agaaatgcct gttccagttc ggtaatggaa ggccgcacca gagggttttt ttcaagtgcc
     4261 cgcaaaataa tccgctccac actttcaggc aaatcagggt taatttcacg cggtggcgac
     4321 ggcatttctt taatctggcg caaaatgaca tcaagcacac cgcgcgatga tggttgaaac
     4381 ggtaaacagc cacaaaccat ttcatacatc atgatgccga cgctatagac gtcagaccga
     4441 ccatcgtagg gtgacccttc cagtcgttcg ggggacatat aggttggggt cccaatgata
     4501 attcccgctg cggtgag
//
LOCUS       DAOQDO010000179         4508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12942_length_4508_cov_2.933977, whole genome shotgun sequence.
ACCESSION   DAOQDO010000179 DAOQDO010000000
VERSION     DAOQDO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12942_length_4508_cov_2.933977"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKZ53_04045"
     CDS             complement(<1..900)
                     /locus_tag="PKZ53_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39638.1"
                     /translation="MIRQRFFLIFFVIVSIFQLNVVLPEQPVGASCHPPHQIARHPFP
                     ARPRPIRPVTRQSTTSELTGKAKQLFELAKASNPERVQFALDKGARVIPTPDGESFAV
                     SWEPSHPAENKPVMIALHGSTSFAFDEFYLWYPSLEKHGVAIIALQWWFGETGTSRDY
                     YQVNELYPIMETFLRERGQQPGTAILQGFSRGSANSYGVTYFDRDTGNNYFGMTFALS
                     GSAELDFPLYADIESGELGEKVFTGTHWALFCGGKDPNPEKTGCPGMERTANWLKKHG
                     GSIDLMFEDPNLAHGGFNWDSEGT"
     gene            1230..>4508
                     /locus_tag="PKZ53_04050"
     CDS             1230..>4508
                     /locus_tag="PKZ53_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ39639.1"
                     /translation="MKRAKNVFLLFVVLIAVGCLLVKLSTHAQQTDYERQYQRLKAQA
                     EKLYQEKSYALANAEYAKINPALLPQSESRWVQFRLADTLWRSQAATETADTSQFDRA
                     QRQLEALVRDIERPEDRDLVWVEIQESLGDFWWLRRQSHNWYQGWQYYQQALDWWANS
                     PEIDVARDRYLKIVFRMSTPPWADQYYYYGYYGSLPLEVAANALAIAQTENDKAHASY
                     LLAATYRYQGGQMDRWERVPSLFEQALKPGKSTDWYDDTLFAYAQWMKDTGVIKWSEQ
                     TGWTQEPDFVKALELYRRLTTEFQKGETRFFDDAQEEIKNMTQPVVEVSVSNIFLPDS
                     EIEYSLNWRNVKQINLAIYQTDLTQDVVFKAGEQQVDTWLETLTLSAKTKVKSWTKET
                     GEKGDYKPGSETLRLDEKLPVGAYLITAEAGQQKSRELILVTNASLVVKTSPTKVIAF
                     LTDAANGKPLASAKLRMWDQYYQNNAWQSRDYQLETDQNGLAQINLVSHTDYTHRIFV
                     AGKSQDHQAFCVAYDQNQSESAKWKVYAFTDRPAYRPNETVNWKFLVRNQADQTYTTP
                     ANQVVEYEITDPRGTKVKADKATLNQFGSAWGTLDLTETMPLGEYRITFWTDGRNLTI
                     GGAMLFRMEEYKLPEFKVSVSAPEENGRKKTFRLGDEVEVAIQADYYFGGPVANATVQ
                     VVVYQKPFHQSWQEPREFPWFYEDSDQNSYRRSSEYGNGQTVKQETIKTDLNGKATLK
                     FETPRDSQQDFEYRVEARVTDASRREIIGSDKIRVTRQRYYVYPRADHWIYQPQDRVT
                     MKFKAIDANSSPVEAEGTVTIWRETWREIWVDPTGREVTGDELLKLQTAGTIFPPQVP
                     KGEKPWKLKARGYKRDEVLKQTVKTNAEGNGELMFTAAQEGYYTINWASTDQDQRPVL
                     AATQIWVSTTNNQDLGFHPQQVQIIVDKDTAQVGRTMPVLLTSPVNDRYILFTIESDE
                     IYSHQVVHVTGTTRLVEVPIDARHVPNVKMTAVMVNEYQVVGAEVELVVPPTENFLKV
                     DIKADRDEYQARQEGTLTVTTRDSSNRPISAEVALGLSDESVLYIQSDYAADPRAWFY
                     GRKRP"
BASE COUNT         1197 a         1131 c         1143 g         1037 t
ORIGIN      
        1 agtaccttcg gagtcccagt taaacccgcc gtgggccagg tttgggtcct caaacattaa
       61 atcaatagag ccgccgtgtt ttttgagcca gttggcggtg cgttccatcc ccggacagcc
      121 agttttttcg ggattagggt cttttccacc acaaaataat gcccagtgcg ttccggtaaa
      181 taccttctca cctagctcac cactttcaat atcggcatac agcggaaaat ccagttcagc
      241 gcttcccgag agggcaaacg tcattccaaa atagttattt ccagtatcgc gatcaaaata
      301 ggtcacgcca tagctattgg ctgagccgcg actaaacccc tgtaaaatgg cggtgcctgg
      361 ttgttggccg cgttctctga gaaaggtttc catgattgga tacaattcgt tgacctgata
      421 ataatctcga ctggttccgg tttctccgaa ccaccactgg agcgcgatga tggccacccc
      481 gtgtttttcg aggcttggat accacagata aaattcatcg aacgcaaagc tggtgctgcc
      541 gtgtaaggcg atcatcacag gtttgttttc ggctggatgt gatggctccc acgaaacagc
      601 aaaactttca ccatctggtg ttggaatgac cctggcacct ttatcaagcg caaactggac
      661 tcgctccgga ttgcttgctt tagctaactc aaacaactgt ttggctttac cagttaattc
      721 actggttgtg ctctgacggg tgaccggtcg aatcgggcgc ggtctggccg ggaatggatg
      781 gcgggcaatc tggtgtggtg ggtggcaact tgctccaact ggttgttcag gaaggacgac
      841 attgagttga aagatggaaa caataacgaa aaatatcaaa aagaagcgct gtcgaatcat
      901 aaatttgttc gctcctgtaa tctcaggctg tgataaagca ttgggtgggc atgagttcct
      961 cgggaaaccc catcgccaca cagagacacc cttgagtttc aggaaagaaa cctgcggaat
     1021 agtttgggag aagctcaggc gtgcttctgt gttggcgtgg agtgaaaagt taattccaaa
     1081 agtgggaact tccccggaaa atgcgcgact aaagataagg ttgtgtttca gaacaaatca
     1141 tctgcactac tctagccctg acattccagc ttttttccct ttctccaggc acaacgaacc
     1201 atcattctca aattggtcag aggtcacgca tgaaacgtgc aaaaaatgta tttttgctct
     1261 ttgtggtatt gattgctgtt ggatgtttgt tggtgaaact gtcaactcac gcccagcaga
     1321 ccgactatga acgccagtac cagaggctca aagcccaggc cgaaaagctg tatcaggaaa
     1381 aatcctacgc tctggccaat gccgaatacg ccaaaatcaa cccggcgctc ctgcctcaat
     1441 ccgaatctcg ctgggtacaa tttcgactgg ccgacacatt gtggcggtca caggcggcga
     1501 cagagactgc cgacacttca caatttgacc gggcgcaacg gcagctcgaa gccctggtgc
     1561 gtgacattga gcgtccagaa gaccgcgacc tggtctgggt tgaaattcag gagtcgctcg
     1621 gtgatttctg gtggttgcgc cgccaatccc acaactggta tcagggctgg caatattatc
     1681 aacaggccct cgactggtgg gcaaattcac ctgaaattga tgtggcccgt gaccgctact
     1741 tgaagattgt ctttcggatg tcaacaccgc cctgggctga tcaatattat tattatggct
     1801 attatggttc cttaccgttg gaagttgccg ccaatgccct ggccattgcc caaaccgaaa
     1861 atgacaaggc ccacgccagt tatttgctgg ctgccaccta tcgttatcaa ggcggtcaaa
     1921 tggaccgttg ggaacgagtt ccatctcttt ttgaacaggc actcaagcct ggcaaaagca
     1981 ctgactggta tgacgatacc ctgtttgcct atgcccaatg gatgaaggac accggggtaa
     2041 tcaaatggag tgaacaaact ggctggacgc aagaaccgga ctttgtcaaa gccctcgaac
     2101 tctaccgccg cttaaccacc gaattccaaa aaggcgagac ccgctttttc gacgatgccc
     2161 aggaagagat caaaaacatg actcagccgg tggtggaggt ttctgtctca aatatcttcc
     2221 tgccggattc tgaaattgaa tactcgttga actggcgcaa cgtcaaacag atcaatctgg
     2281 ccatctatca aaccgatttg acccaggatg tggtcttcaa agctggcgag caacaggttg
     2341 atacctggct ggagaccctc acactttcgg cgaaaaccaa agtgaaatcc tggacgaaag
     2401 aaactggtga gaaaggtgac tataagcccg gctccgaaac acttcgactg gatgaaaagc
     2461 ttcctgtcgg cgcctatctc atcacggctg aagctggtca gcagaaatcc cgcgagttga
     2521 ttctggtgac caatgcttcg ctggtggtca agacttcacc gacaaaagtg attgccttct
     2581 tgacggatgc tgcgaatgga aaaccgcttg ccagtgcgaa actgcggatg tgggatcaat
     2641 attatcaaaa caatgcctgg caaagtcgtg attatcagct cgaaactgac caaaacgggc
     2701 tggcccaaat caatctggta tcacataccg attacactca tcgaatcttt gtggctggga
     2761 aaagccagga ccatcaggca ttttgcgtcg cttacgacca gaaccagagt gagtcagcca
     2821 aatggaaagt gtatgccttt actgaccggc ccgcttatcg gcctaatgag acggtaaact
     2881 ggaaattcct ggttcgcaat caggctgatc aaacctacac aaccccagcc aatcaggtgg
     2941 tcgagtatga aatcaccgac ccacgcggaa ccaaagtcaa agctgacaag gccacactca
     3001 atcagtttgg cagtgcctgg ggaacgctgg acctgaccga aaccatgccg cttggggaat
     3061 atcgcattac gttttggacc gatgggcgca acctcacgat tggcggagca atgctctttc
     3121 gaatggaaga atacaaactt ccggaattta aagtctcggt gagtgcgcct gaggaaaacg
     3181 gacgcaaaaa gaccttccgg ctgggtgatg aagtcgaagt cgccattcag gccgattatt
     3241 attttggtgg accagtggcc aatgcgacgg ttcaggtggt tgtctatcag aagccgtttc
     3301 atcagtcctg gcaggagcca cgtgaatttc cctggtttta tgaagactct gaccaaaatt
     3361 cctatcgccg aagttccgaa tatggaaatg gtcagacggt aaaacaagaa acaatcaaaa
     3421 ccgacctgaa cggaaaagcc acacttaaat ttgaaactcc acgtgatagc cagcaggact
     3481 ttgaatatcg ggttgaagct cgcgtgacgg atgcttcgcg gcgtgaaatc atcgggagcg
     3541 ataaaattcg ggtcacccgc cagcggtatt atgtctaccc gcgagccgat cactggattt
     3601 atcaaccgca agatcgggtg acgatgaaat tcaaggccat tgatgccaac tcgtcgccag
     3661 ttgaagccga aggaaccgtt accatctggc gcgaaacgtg gcgtgaaatc tgggttgatc
     3721 caaccggacg tgaagtaacc ggcgatgaat tgctcaagct gcaaaccgct ggaacgattt
     3781 tcccgcccca ggttccaaaa ggtgaaaaac cctggaaact taaagcgcgg ggctacaaac
     3841 gcgacgaagt cctcaaacaa accgtcaaaa ccaatgctga aggcaacggt gaactgatgt
     3901 ttacggcggc acaggaaggg tactacacga tcaactgggc gagtactgac caggatcagc
     3961 gcccggtgtt ggctgctact cagatttggg tttcaaccac aaacaaccag gatcttgggt
     4021 tccatccaca acaggttcaa atcattgtag ataaagacac ggcacaagtt ggccgtacaa
     4081 tgccggtgtt gctgacgtca ccggtaaacg accgatatat tttgttcacg attgaaagcg
     4141 acgaaattta cagtcatcag gttgtccatg tgaccggaac gacccggctg gttgaagttc
     4201 ccattgatgc ccgacatgtt ccaaacgtga aaatgaccgc cgtgatggtc aacgagtacc
     4261 aggtggttgg tgccgaagtt gaactggtcg ttccgccaac cgagaatttc ctgaaagtgg
     4321 acattaaagc cgaccgcgat gaatatcagg ctcgtcagga aggcaccttg accgtgacga
     4381 cccgcgattc cagcaaccgc cccatttctg ccgaagtggc gcttgggttg tcagatgaat
     4441 cagttctgta tattcagagt gactatgccg ctgacccacg tgcctggttc tatggccgca
     4501 agcgtccg
//
LOCUS       DAOQDO010000180         4506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12951_length_4506_cov_3.360818, whole genome shotgun sequence.
ACCESSION   DAOQDO010000180 DAOQDO010000000
VERSION     DAOQDO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12951_length_4506_cov_3.360818"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1952
                     /locus_tag="PKZ53_04055"
     CDS             <1..1952
                     /locus_tag="PKZ53_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNJ39640.1"
                     /translation="WAGFQLARRPAFLTLSRETAILALLFLPSSEFMHTFWRLVRYVR
                     PYVGVLLLAVILTACVGLFEAARTALIKPILDGLTASSPVAVGTSPPVSVGLTTPTAS
                     QSPFLIDRWLPHGSAFWSSILGLMISFTFLRGVSQFFSNYLLTTIGQKVIVHLRQDLF
                     AHLLAQSNSFFHQHRTSDLTAHIISDVEKTQLAVSLYLADALREGFTLICLLVLAFVL
                     SWKLTLVSLAVVPFVATLTARFGKRLRMTSHATQEGVQEVLGITQETLSHQQIVKAFG
                     AEKFEEQRFLVVSERLRRSNLRTARALFLPSPIMDLMGVGIGAVVIYYTQHLIATGQI
                     TVGGVTATLAALFQLYDPVRKLSQTHNAYSQVAAAAARVFRLLDQHTEIQDLPDAIEL
                     TSFSNRIEFSDVTFTYPGTSTPALDRVNLVIEKGQMVALVGRTGSGKSTLLNLLLRFY
                     DCDSGVVLIDGVDVRAAKLASLRAQMAMVSQESVLFQESFQYNIAYGAKEIDPKAVEE
                     AAKAAYAQEFIEERGGYRVNVGESGKEISGGQRQRIAIARALYKNAPILLLDEATSHL
                     DTESERIVQQALTNLTRERTTLVIAHRLSTIQQADKIVVLNAGQIVETGTHDQLLATN
                     GLYRRLYDLQFVDESVVSSPSVSRQ"
     gene            2130..3005
                     /locus_tag="PKZ53_04060"
     CDS             2130..3005
                     /locus_tag="PKZ53_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNJ39641.1"
                     /translation="MKSMTGFGSAKCELQDFTVNVDVRTVNSRFLDVVMRLPVEFSGQ
                     DNRVKKQVQSVLRRGRVEVSVSVQPNREVSYDVNLPLVRGYVSALRRAQEATGVDGML
                     DLSLIARLPGAIQSAALGPDLVEQLATGLTTALGQALENLEQMRLAEGTELAAELTLR
                     LEHIARRVTEVEAMVPQVVEAYQQKLERRITELLRDKGEVDPVRLAQEAAFLADRSDV
                     TEEIARLKSHLTQFNQLVGTQGELGKQMDFLTQEMNREANTILSKSGDVPITQAALAI
                     KMEVEKIKEQVQNVE"
     gene            2995..3654
                     /gene="gmk"
                     /locus_tag="PKZ53_04065"
     CDS             2995..3654
                     /gene="gmk"
                     /locus_tag="PKZ53_04065"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNJ39642.1"
                     /translation="MSNEADVRKIPARRGGLIVISAPSGAGKTTLLARLLKSVDNLGY
                     SISYTTRAPRPGEQHGKDYFFVSVDEFLRMRERGEFLESAYVHGNFYGTSRRVIEDEL
                     ARGRDIVLDIDVQGASLISQVMPHSAQVFIMPPGFDVLAQRLRSRGLDKPEDIEQRLR
                     NARDEVIRFREFHYVIINDDLEAATQYLISIVHAERVRWRRSEHILRNLVSTFEGVVK
                     R"
     gene            3651..3875
                     /gene="rpoZ"
                     /locus_tag="PKZ53_04070"
     CDS             3651..3875
                     /gene="rpoZ"
                     /locus_tag="PKZ53_04070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNJ39643.1"
                     /translation="MTEKDKKSLHYADSKYRLILIAARRSKQIQKGDSPRLVSNSHKP
                     TRVALEEVHKGLIPFEILPPRKAKEVEVEF"
     gene            4009..>4506
                     /locus_tag="PKZ53_04075"
     CDS             4009..>4506
                     /locus_tag="PKZ53_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39644.1"
                     /translation="MSKPATVRPRPIDRVRKAIIAQYPLLYLLSWEEDRVERMIQSIA
                     RGFNPPLNVYIWTISQGLRLGEELIPGTMDARGVLEFIIKTQDRGFFLLKDFHREVEN
                     NPLIIRELRDAYYALKEKGKFLCLVSPILSIPDELKKEIYLVEVLLPAEDEIGPLVDY
                     LTTRYF"
BASE COUNT         1058 a         1129 c         1180 g         1139 t
ORIGIN      
        1 tctgggcggg ctttcaactt gccaggcgac cagctttctt gacgctttcc cgggaaactg
       61 cgatactcgc gcttcttttt ctcccctcat ctgagtttat gcatactttt tggcgacttg
      121 tacggtatgt ccggccctat gtcggcgtcc tgcttctggc agtaattttg acagcgtgtg
      181 ttggtctttt cgaagcagcc cggactgcct tgatcaagcc aatcctcgac ggactgaccg
      241 cctcttcacc ggttgcggtc gggacctctc cgccagtttc agttggactg accaccccca
      301 ctgcttccca gtcccccttc ctgattgacc gctggcttcc gcatggatct gcgttctggt
      361 cgtccatcct tggattgatg atcagcttta cttttttgcg tggggtcagc cagttttttt
      421 caaattattt gctgacgacc atcggccaaa aagtcattgt ccatctgcgc caggatctgt
      481 ttgctcattt actggcccaa tcaaatagtt tttttcacca gcaccgaacc tcagacctga
      541 ccgcccatat catcagcgac gttgaaaaaa cacaactggc ggtttcgctc tatctggcgg
      601 atgcgctccg ggaaggtttt accctgattt gtctgctggt tctggcattt gttctgagtt
      661 ggaaactgac cctggtttcg ctggcggttg tgccctttgt ggcgacgctc accgcccggt
      721 ttggcaagcg cctgcgaatg acgagccatg ccacccagga aggtgttcag gaagttttgg
      781 ggattaccca ggaaactctt tcacatcaac aaattgtaaa agcatttggg gctgaaaaat
      841 ttgaagagca gcggtttctg gtagtttctg aacgcctgcg ccgatcaaat ctgcgcaccg
      901 cacgtgcgct ctttttgcca tctccgatta tggatttaat gggcgtcggc attggggccg
      961 tggtgattta ttacacccag catctgattg cgaccgggca aattacggtc ggaggagtga
     1021 cggcaacgct ggcggcactc tttcagcttt atgatccggt ccgcaagctc agccagacgc
     1081 acaatgccta tagccaggtg gccgccgctg ctgcgcgggt cttccggctg cttgatcagc
     1141 acaccgagat ccaggattta ccagatgcaa ttgaactgac ttctttttcc aaccgaattg
     1201 agttttcaga tgtcaccttt acctacccag gcacctcaac cccagccctt gatcgggtaa
     1261 atctggtcat cgaaaaagga cagatggtgg cactggtcgg gagaaccggt tctggaaaaa
     1321 gcaccttgct caatttgttg ctgcggtttt atgactgtga ttccggggtc gtcttgattg
     1381 atggtgtgga tgtgcgggcc gcaaaactgg cttcgttgcg agcgcaaatg gcgatggtgt
     1441 cacaggaaag cgtgctgttt caggaaagct tccaatataa tattgcctat ggtgccaaag
     1501 aaattgatcc aaaagcagtt gaggaagccg ccaaagccgc gtatgctcaa gagtttattg
     1561 aagaacgtgg cggatacagg gtcaatgttg gtgagtcagg caaggaaatc tcaggcggtc
     1621 aacgccagcg cattgccatt gcccgggcgc tttataaaaa tgctccgatt ttattgctcg
     1681 acgaagcgac ctcgcatctg gacaccgaat cagaacgcat cgtgcagcag gccttgacca
     1741 atttgacccg cgaacgcacc acgctggtga ttgcccatcg cctttcgacc attcaacaag
     1801 ctgataaaat tgtggttctc aacgctggtc agattgtcga gactggcacc cacgatcaac
     1861 ttctggccac caacggattg tatcgtcgtc tctatgacct tcagtttgtt gatgagtcag
     1921 ttgtcagtag tccatcagtc agtcgtcagt agtcagtaga taaaacccag atcgttgaat
     1981 ccatagaatg aattgttcag aactgactca catagaagca aatagacttg accggcaatt
     2041 caaaacccca accctcgacg actgacgact gacgactgac gactgacgac tgacgactga
     2101 cgactgactt ccaactggaa taaaaactca tgaaaagcat gacaggtttt ggtagcgcaa
     2161 aatgtgaact gcaggacttt accgtgaatg ttgatgtgcg aacggtcaac agccggtttc
     2221 tcgatgttgt gatgcgcctt ccggtcgaat tttcgggcca ggacaaccgg gtaaaaaagc
     2281 aggttcagtc agttctcaga cgtggtcgag ttgaagtttc ggtttccgtt cagccaaacc
     2341 gggaagtcag ttatgacgtc aaccttccgc tggtgcgtgg ctatgtttca gccctgcgcc
     2401 gggcccagga agccacgggg gttgacggca tgcttgattt gagtttgatt gcccggttgc
     2461 caggggcgat tcaatccgcc gcgcttggac ctgacctggt tgagcagctt gccaccgggc
     2521 tgaccacggc gctggggcag gcactggaaa acctggagca gatgcgtctg gctgaaggca
     2581 cggagcttgc cgctgaattg acgcttcgat tagagcacat tgcccgccgg gtgactgaag
     2641 tcgaggccat ggtgccgcaa gtcgttgagg cgtatcagca aaaactcgaa cgccgcatta
     2701 ctgaattact ccgggataaa ggcgaagttg acccggtgcg attagcccag gaagccgcct
     2761 ttttagcgga tcgcagcgat gtaaccgaag aaatcgcccg gctcaaaagc cacctgaccc
     2821 agtttaacca gcttgttgga actcagggcg aacttggaaa acagatggat tttctcaccc
     2881 aggaaatgaa ccgcgaagca aataccattc tttcaaaatc tggcgacgtc ccaattactc
     2941 aggcggcgtt agcaatcaag atggaagtcg agaaaattaa ggaacaggtg caaaatgtcg
     3001 aatgaggctg atgttcgaaa aatacctgcc cggcgcggtg gattgattgt gatttcggca
     3061 ccgtctgggg ctggaaaaac gaccttgctg gctcgattgt taaaatcggt tgataacctg
     3121 ggctattcta tttcttacac cacccgcgca ccacgccctg gggaacagca cgggaaagac
     3181 tacttttttg tttctgtgga tgaatttttg cggatgcgcg agcgcggaga atttctggaa
     3241 tcggcctacg ttcacgggaa tttttacggc accagtcggc gagtcatcga agacgaactg
     3301 gcccgtggcc gagatattgt tctggatatt gatgtgcagg gcgcctcgct catcagtcag
     3361 gtgatgccgc attcggccca ggtgtttatt atgccgcctg gttttgacgt tttggcgcaa
     3421 cggttacggt cacgtggact ggataaacct gaagacattg aacaacgctt gcgcaatgct
     3481 cgcgacgaag ttatacgttt tcgggaattt cactatgtga tcatcaatga tgatttagaa
     3541 gctgccaccc agtatttgat ttcaattgtt cacgctgaac gcgtccgatg gcgacgttca
     3601 gaacatatct tgagaaacct ggtttccact tttgaaggag tagtaaaacg atgactgaga
     3661 aagacaaaaa atcgctgcat tatgccgaca gcaaataccg tctgattttg atcgctgccc
     3721 ggcgcagcaa acagattcag aaaggcgatt ctccacggtt ggtttccaat tcacacaaac
     3781 cgacccgggt tgcgttagag gaagtccaca agggattgat tccgtttgaa atccttccgc
     3841 cccgaaaggc aaaagaagtc gaagttgagt tttaggcgtg atcagaaaga ttatgggtca
     3901 gtaccgcccg cctgagcagg tgggttcgga aatcaatgag gcccaccccc tgacgcgggt
     3961 ggtactgacc aattccttat ttgtacccct gatctggcga aattctttat gagcaaacct
     4021 gcaactgtcc gtccccgccc gattgaccgt gttcgaaaag cgatcattgc ccaatacccg
     4081 ctgttgtatt tgctgagttg ggaagaagat cgtgttgaac ggatgattca atcaattgcc
     4141 cgagggttta acccgccgct caatgtctat atctggacga tttctcaagg gttacggctc
     4201 ggggaagaat tgattcccgg cacgatggat gcccgggggg tactggaatt catcatcaag
     4261 acccaggatc gtggcttttt tctgctcaag gattttcacc gcgaagtcga aaacaacccg
     4321 ttgattatcc gcgaattgcg tgatgcctat tatgcgctga aggaaaaagg caagtttttg
     4381 tgccttgtct cgccaatctt gagcattcct gacgaactga aaaaagaaat ttatctggtc
     4441 gaagtgctgt tgccggctga agatgaaatc ggcccgctgg tggactatct caccacccgc
     4501 tatttc
//
LOCUS       DAOQDO010000181         4501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12976_length_4501_cov_3.561853, whole genome shotgun sequence.
ACCESSION   DAOQDO010000181 DAOQDO010000000
VERSION     DAOQDO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_4501_cov_3.561853"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="PKZ53_04080"
     CDS             complement(<1..2353)
                     /locus_tag="PKZ53_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39645.1"
                     /translation="MKSKLQAVFLLCLLGISLLVLPPQPVRACGPDFGTARFIFTKHP
                     DFPLRRFARGELNVLQPAYAWSYLIVAYRYLNGIGLDEIEQEAVIAKWETRLGISQEK
                     KSDYWLNQWLDARKAVSNAPASPKISEFVKEGDSYSAEIAITAEAFQVAIRTLNDRIK
                     QFGPTSPQVREWLKAQDQVFQTASGEPSIPEAPAASLDTVIKADRAYQIAAANFYANE
                     HELAVKGFDEIAKDGKSPWKTMAPYLAIRTLNRKFEKQIQTTPEEQAKLFGDIRDRSA
                     KVLADKQLSEYHAATRRLLADVQLAEIAAKSGSTESGAPTPEQTQAEVAVLEPITLDL
                     ARDLMRPHSGSNIGRNLWNFPNRLDEIIEKTTESGSFWDTVDFDRVNRKFKTLPAIRQ
                     KDDLIDWILVFQTMDDEARDYAIQTWEKTGKLHWLCAALTKATGDSPKLANLISAAER
                     VPADSPASTLTTYHRLRLLVETGKLDDARKGLAEFIKTKGNRLTQSSVNLFSQLQMHT
                     ATNLTELAKNLSRHPAGITNSFDYFQLPADFLEVYPDWPESEQIKKERQEEETQFLFD
                     VQAARVLNQGLPLSQLGALLQDTALPKNLRGNLALAVWVKAGLLENRDVATQASLVVD
                     KLVPELKDMTSAYRAANTAPEAKFALIFAVLRFPGLRPHIVNGLERTETLDTIDSYRD
                     NWWCNFDGKLEVSSGNFEKFNYYDPDQEYGPDGEPIPKPEQPFDPAKAFFPPAFLTAE
                     QKEAAFKEWKTLVAIGTAPNYLCRQTIDWAKKNPTDARVPEALH"
     gene            complement(2350..3150)
                     /locus_tag="PKZ53_04085"
     CDS             complement(2350..3150)
                     /locus_tag="PKZ53_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39646.1"
                     /translation="MKKPVLILLALVLITAGASFFFSRKNIKTQKSFSVDCQKPRTQA
                     IPRLMLWAWERPEDLRFLDPTQAGVAYLAATLTLTGDRVAVRPRLQPLKVAQGAYVMT
                     VVRIEVSQDDRPTLSDPQRTQVVAEILKAMKSPNVAALQIDFDARQSERGFYRALLTD
                     VRAQLPQTMPLVMTALASWCLFDTWTDDLPVDDVVPMVFRMGKDTENVVKFLASNQGF
                     RSQLCGCSVGIALDELRPEIPTNRRVYAFNLRSWTPQQFKFLSEKVLP"
     gene            complement(3160..3663)
                     /locus_tag="PKZ53_04090"
     CDS             complement(3160..3663)
                     /locus_tag="PKZ53_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39647.1"
                     /translation="MALDARFLRAEEFGIRLELVGGLPLWEPYPVYKHQKAIDRIRAT
                     IEPIPELADPPSSACACIHISDVYVSFSDGSLKRPDIAIFCREPDEEDEAITLIPEAV
                     IEVISKGYEAKDLDIGPKFYLANGVKDVVVLNPYSGEVTLFTTSQTQHFVSPVTLSLQ
                     CGCRCTV"
     gene            complement(3783..4274)
                     /gene="ybaK"
                     /locus_tag="PKZ53_04095"
     CDS             complement(3783..4274)
                     /gene="ybaK"
                     /locus_tag="PKZ53_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNJ39648.1"
                     /translation="MVKIDYPVTPAIRLLRDKKIAFEPHLYDYEERGGTKHSAEALGV
                     DEHIVVKTLVLENDARKPLIVLMHGDCEVSTKQLARILGVKSISPCDPATAQKHSGYL
                     VGGTSPFGTKATMPVYVERTIFSLPKIYLNGGKRGFLISIDPQDLKKVLPVTEVEVAI
                     SSR"
BASE COUNT         1093 a         1127 c         1171 g         1110 t
ORIGIN      
        1 gatggagcgc ttcgggaacc cgcgcatcgg tcggattttt ctttgcccag tcaattgtct
       61 ggcggcagag gtagttaggg gcggtgccaa tcgccaccag ggttttccat tctttgaacg
      121 ctgcttcctt ttgttcagcc gtcaaaaagg ctggtgggaa gaacgccttg gccgggtcaa
      181 acggttgctc tggtttagga atcggttcgc catccggtcc atattcctga tctgggtcat
      241 agtagttgaa cttctcaaag tttccggatg aaacttcaag cttaccgtca aaattgcacc
      301 accagttatc gcggtaactg tcaattgtat caagtgtttc cgtgcgttcc aggccgttca
      361 caatatgcgg acgcaggcca gggaagcgta agaccgcaaa aatcagggca aatttggctt
      421 ccggtgcggt attggcagcc cggtaagcag acgtcatgtc tttgagttcc ggcaccagct
      481 tgtcaaccac aagtgatgcc tgggtcgcga cgtcacggtt ttcaagcaac ccggctttga
      541 cccaaacggc cagcgccaga tttccacgca gatttttggg gagtgcggta tcttgaagca
      601 gggcgccaag ctgactgagc ggcagccctt gattgaggac gcgcgcggcc tgaacatcaa
      661 agagaaactg ggtttcttct tcctggcgtt ctttcttgat ttgctcagat tcaggccaat
      721 ccggatagac ctctaaaaaa tcagccggca actggaaata atcaaacgaa ttggtaatcc
      781 ccgccgggtg acggctgaga tttttggcaa gttcggtcag attggtggcc gtatgcatct
      841 ggagttgtga aaacagattg actgaagatt gggtcaacct gttgcctttg gtctttataa
      901 actcagccag tcctttgcgg gcatcatcga gtttcccggt ttcaaccagt aaccgcaggc
      961 gatgataggt tgtcagggtg gaggccggtg aatccgctgg aactcgttcg gcggctgaaa
     1021 tcagattggc cagctttggc gaatctcccg tggctttggt caatgcagca cagagccagt
     1081 gcagtttccc ggtcttttcc caggtttgta tcgcgtagtc tctggcttca tcgtccatcg
     1141 tctgaaacac aaggatccaa tcaatcaaat catctttctg gcgaatggcg ggcagggtct
     1201 taaattttcg attgacccga tcaaaatcca ccgtatccca gaaactgccg gattcagttg
     1261 tcttttcaat aatttcatcc agtcggtttg gaaaattcca caaattccgc ccaatgttgg
     1321 accccgaatg cggacgcatc aggtctctgg ctaagtcgag ggtaatgggt tcgaggaccg
     1381 ctacttcagc ctgggtttgt tccggagttg gcgcgccaga ttcagttgag ccagatttgg
     1441 cggcaatctc ggccagttga acatctgcca gcaggcgtcg cgttgcggcg tgatactcgc
     1501 tcaattgttt gtctgccagc actttggcgc tccggtcgcg gatgtctcca aagagtttgg
     1561 cctgttcttc gggcgtggtc tgaatctgtt tctcgaattt tcggtttagt gtccgaatcg
     1621 cgagatacgg agccatcgtt ttccagggcg atttgccatc tttggcgatc tcgtcaaagc
     1681 ctttgacggc cagttcgtgt tcattggcat aaaagttggc cgccgcaatt tgataagccc
     1741 ggtcagcttt aatcactgtg tcaagcgagg cggctggtgc ttcagggatt gacggttcgc
     1801 cgctggcggt ctgaaacacc tgatcctgtg ccttgagcca ttcgcgaacc tgggggctgg
     1861 ttgggccaaa ctgtttgatt cggtcgttga gggtacgaat ggcaacctga aaggcttcgg
     1921 cggtgatggc gatttctgct gaatagctgt caccttcttt gacaaattct gaaatttttg
     1981 gtgaggccgg agcgtttgaa actgccttcc gggcatcaag ccactggttg agccagtaat
     2041 cgcttttctt ttcctgactg atacccaatc gggtctccca tttggcgatg acggcttcct
     2101 gctcaatctc atccagtccg atgccgttca aatagcgata ggcgacaatc aaatacgacc
     2161 aggcataagc tggttgcagg acgttgagtt caccacgggc aaagcgtcga agcggaaaat
     2221 caggatgttt ggtaaagata aagcgagcgg tcccaaagtc tggaccgcac gctcgaaccg
     2281 gttgcggtgg aagaacaagc agggaaattc ccaaaagaca caacaaaaac acagcctgaa
     2341 gcttggattt catggcaata ccttttctga gagaaacttg aattgttgcg gcgtccacga
     2401 gcgaagatta aatgcgtata cacgtcggtt ggttgggatt tccggacgaa gttcgtcaag
     2461 ggcgatacca acgctgcatc cgcacaactg tgaccgaaag ccctggttgg atgcaaggaa
     2521 cttcacaacg ttttccgtat ctttgcccat tcgaaacacc atcggcacga cgtcatccac
     2581 cggtaaatca tcagtccagg tatcaaacaa acaccacgaa gccagcgccg tcatcaccag
     2641 cggcatcgtc tgaggaagtt gggcccgcac atcggtcaac agcgcacgat agaaccctct
     2701 ttctgattgt cgggcatcaa aatcaatctg gagcgccgcg acatttggtg atttcatcgc
     2761 tttcaagatc tcagccacaa cctgtgttcg ttgggggtca ctcaacgttg gtcggtcatc
     2821 ttgactgacc tcaatccgaa ccaccgtcat cacataggcc ccttgagcaa ctttgagcgg
     2881 ttgtaagcgc ggacgaaccg caaccctatc accggtcagg gtcaaggtcg ccgccagata
     2941 ggcgacaccc gcctgggtcg gatcaagaaa ccgaaggtct tcagggcgtt cccaggccca
     3001 gagcatcagc cggggaattg cctgagtacg gggtttttgg caatcaaccg aaaaactttt
     3061 ctgagttttt atgtttttac gcgaaaagaa aaagcttgct ccggcggtaa tcaacaccag
     3121 agcaagcaga attagaaccg gttttttcat gatcaaaggt taaaccgtac accggcaacc
     3181 acattggagt gacagtgtca ccggtgagac aaagtgctgc gtctgtgatg tggtaaaaag
     3241 ggttacttcg ccagaatagg gattcaaaac gacaacatct ttcaccccat tggcaaggta
     3301 aaatttgggc ccaatgtcta aatctttggc ttcataccct ttgctgatga cttcaatgac
     3361 ggcttctgga atcaacgtga tggcttcgtc ctcttcatca ggttcacggc aaaaaatggc
     3421 aatgtccggg cgcttcagag atccgtccga aaaactgaca tacacatcag agatatgaat
     3481 acaggcacaa gctgagcttg ggggatcagc aagctcagga attggctcaa ttgtagctcg
     3541 aatccgatca atcgctttct ggtgcttata caccggatag ggctcccaca gaggtaatcc
     3601 cccaaccagt tcaagccgga ttccaaattc ttcagcgcgt aaaaaccgtg catcaagggc
     3661 cataaaatac catttgggtt cagggttcag agtaacgcct tcaggcgtga ggagtattgg
     3721 gtgggaggga agaaaaaatc tctcgtctaa agacgatact ctgaaccatt tcctgaaaag
     3781 ctctagcggg atgaaatcgc aacctcgacc tcggtgaccg gaagcacttt cttcagatcc
     3841 tgcggatcaa tcgaaatcaa gaagccgcgt ttgccgccgt tgaggtagat tttcggcaac
     3901 gaaaaaatgg ttcgttcgac atacaccggc atggtggctt tggtaccaaa cgggctggtg
     3961 cctccgacca gatagccgga atgcttttgt gccgttgccg gatcacaagg agaaatgctc
     4021 ttcacaccga gtatccgggc cagttgtttg gttgaaactt cacaatcgcc atgcatcaac
     4081 acaatcaatg gcttgcgagc atcgttttca agcaccagcg ttttgacaac gatgtgttcg
     4141 tctacgccaa gtgcttcagc cgaatgtttg gttccgccac gctcctcata gtcatagagg
     4201 tgaggttcaa acgcaatttt cttatcacgc aaaagccgaa tcgctggggt aacggggtaa
     4261 tcaattttga ccatagtagg gttcagggtt cagggttcag ggttcagggt tcaggggaaa
     4321 tcgccagatt gaagaataaa ctcaaactca aattattcat tcctcagccc ttagccccaa
     4381 gccctcagcc ccagtatttt aaaatcgaaa ccgaacctgg gcttcaattc gccgcgactc
     4441 agaggccgaa ttcgccagcc caaagatcgg ggaagtcagt gatccgctaa agttattcag
     4501 a
//
LOCUS       DAOQDO010000182         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_12981_length_4500_cov_3.361980, whole genome shotgun sequence.
ACCESSION   DAOQDO010000182 DAOQDO010000000
VERSION     DAOQDO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12981_length_4500_cov_3.361980"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKZ53_04100"
     CDS             complement(<1..369)
                     /locus_tag="PKZ53_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNJ39649.1"
                     /translation="MTNQKPTRLIQFSWMVLAYNIGVILWGAYVRATGSGAGCGNHWP
                     LCNGEVFPRAKEIETLIEFAHRTSSGLALIFVVALGIWVFRAFPKKHIARTGAMLSLF
                     FIITEALIGAGLVLFKLVAGN"
     gene            complement(636..1568)
                     /gene="cyoE"
                     /locus_tag="PKZ53_04105"
     CDS             complement(636..1568)
                     /gene="cyoE"
                     /locus_tag="PKZ53_04105"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNJ39650.1"
                     /translation="MIETVVPTIPNEKLPAVRSTLIYGLRKAKAYLDLTKPRITFEVV
                     LIATFGYLLGTRGPVDYVLMLHTLCAVALLSSGISTLNQYLERHLDGLMKRTLHRPLP
                     TRKVSETEALVVGLVLAIVAEWYLYFAVNELTAWLGALTAIGYVGIYTPLKTKSTIST
                     LIGAFPGAVPPIIGWVAASGTLSPEAWALFAIMFFWQYPHFLAIAWMYREDYSRAAIK
                     MLPVVEPDGKRTKRQILWNTLILLPLTLAPTLMHLTGIFYAVAAVVLGGYFLRSVLIL
                     NKTGTGQDARRVMKASVIYISILFCVMVIDKMTP"
     gene            complement(1581..1877)
                     /locus_tag="PKZ53_04110"
     CDS             complement(1581..1877)
                     /locus_tag="PKZ53_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNJ39651.1"
                     /translation="MAGHVVPLKTYLSVFGALMVLTGVTVWIATFDLGVWNAPLALAV
                     ATTKMVLIVLFFMHVKYSTTLTKVFVASSFFFVAILFFFTFIDYFSRHLDYLPR"
     assembly_gap    1890..1989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2002..2682)
                     /locus_tag="PKZ53_04115"
     CDS             complement(2002..2682)
                     /locus_tag="PKZ53_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNJ39652.1"
                     /translation="MSTHSNSLAVLHHFADAEQQHESATLGMWSFLATEVLLFGGLFL
                     AYSLFRYYHHDTFVIGSYELGIKLGLTNTIVLICSSLTMALAVHAAQEGHKGKLMGFL
                     VLTMILGAAFMGIKAIEYTEHYHHNKVPGLNFVYNSLPNGGVDATEPVFHFLYFCMTG
                     IHALHMVVGMGLLATILYMSWKDTFSPEYSTPVEMAGLYWHFVDIVWVFLFPLLYLTG
                     LHYKGGGH"
     gene            complement(2686..>3398)
                     /locus_tag="PKZ53_04120"
     CDS             complement(2686..>3398)
                     /locus_tag="PKZ53_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNJ39653.1"
                     /translation="IPSGVKVFNWTATLYKGSISFDTPMLYALGFIGLFIIGGLTGLF
                     LASMGLDQHLHDTYFVIAHFHFVMVGGAVMAYFGGLHYWWPKMFGRMYPEGWGRLSAL
                     IVFIGFILTFFPQFVVGYLGMPRRYYIYAPEFQVWNVLSTAGSTILAIGYILPLFYLF
                     WSLKYGPVVDNPWNAKGLEWTVASPPPWDNFAETPVVTEDAYNYPLHPMGEDPQLAPG
                     QDPMPGYRMPSETGATGD"
     assembly_gap    3399..3408
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3409..4390)
                     /locus_tag="PKZ53_04125"
     CDS             complement(<3409..4390)
                     /locus_tag="PKZ53_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNJ39654.1"
                     /translation="MTPAATVKFPEKHYLNEGFGWKSWLLTTDHKRIGLLYLVSITLM
                     FFLGGFFAFLIRLQLLTPHGEFLKGDTYNKIFTMHGVVMIFFFLIPSIPATLGNFLLP
                     LMIGAKDLAFPKINLLSWYIFIVGAIYVLWIIMVGGLDTGWTLYTPYSTKYSNTNVIA
                     ATLGVFVLGFSSILTGLNFIVTTHKMRAPGLTWFRMPLFVWALYATSLIQILATPVLA
                     ISMVLLAAERFAGIGIFDPALGGDPVLFQHMFWFYSHPAVYIMILPSLGVVSELIAAF
                     ARKKPFGYKFIAVSSMMIAIFGFLVWGHHMFVSTMSVYAGMVFSFITYFVA"
BASE COUNT         1241 a         1138 c         1094 g          917 t
ORIGIN      
        1 attcccggca accagtttaa acaaaaccag tccagcacca attaaggctt ctgtgatgat
       61 aaaaaagagc gaaagcatgg ccccagttcg ggcaatgtgt ttttttggaa aggcacgaaa
      121 gacccaaatg ccgagggcaa ccacaaaaat gagcgccagc ccgctactgg tccgatgagc
      181 aaactcaatc agcgtctcaa tttctttcgc tcttgggaac acttctccgt tacacagcgg
      241 ccagtgattg ccgcaaccgg cgccagaacc agttgcccgg acataggcgc cccataaaat
      301 gacaccgatg ttataggcca ggaccatcca ggaaaattga atcaaacggg taggcttttg
      361 attggtcata tggtttaggg cttggggctg agggctgagg gtatggggct tggggttttt
      421 caaggctaag tggttggaaa acaggtgctt ccctcagctc ttagccccat accctcagcc
      481 ccacctggta tttccctcag cccttaaccc tataccctca gccctaaatg aaaaagggtg
      541 aacattgata agcgatgctg gtcaagtttg cacctgtcca gacaactcac cgctcccgtt
      601 cacccctgca cttccgtttt ttgatggtta tcaggctatg gggtcatctt gtcgatgacc
      661 atcacgcaaa acaaaatcga aatataaatg accgatgcct tcatcacgcg tcgggcatcc
      721 tgcccagtac cggtcttatt caaaatcagc acactgcgca ggaaatatcc ccctaacacc
      781 accgccgcga cggcataaaa aattcccgtc aggtgcatca gggttggtgc cagcgtcaac
      841 ggcaacagaa tcaaggtatt ccacaaaatc tggcgtttgg tgcgctttcc atcgggttca
      901 accaccggga gcattttaat cgccgcccgg ctgtagtctt cacggtacat ccaggcaatg
      961 gccagaaaat gcggatattg ccagaaaaac ataatggcaa acagcgccca ggcttcaggt
     1021 gacaatgttc cagacgcggc gacccagcca atgattggtg gaacggcacc tggaaaggcc
     1081 ccaatcaggg ttgaaattgt tgatttggtt ttgagtgggg tataaatgcc aacgtatccg
     1141 atagccgtca gggcaccgag ccaggcagta agttcattga ccgcaaagta caggtaccat
     1201 tcagccacaa tcgccaggac cagcccaacc accagggctt cggtttcaga gacttttcga
     1261 gtgggaagtg gacggtgaag ggtgcgtttc atcaagccgt ccaggtggcg ttccaggtat
     1321 tgattcaatg tcgaaatacc tgatgaaagc agggcgacgg cgcacagggt gtgcagcatg
     1381 agcacataat caaccggccc acgcgtgccg agcagatacc cgaaggtggc aatcaagaca
     1441 acttcaaaag taatccgtgg cttggtgagg tcaagatagg ctttggcttt gcggagtccg
     1501 taaatcaaag tgcttcgtac cgctgggagc ttttcatttg gaatagtggg gacaacggtt
     1561 tcaatcattc cacaaagcct ctaccttggc aaatagtcca ggtggcgcga gaagtagtca
     1621 ataaaggtaa agaagaacaa aatcgccaca aaaaagaagc tcgacgccac aaaaaccttg
     1681 gttaaggtcg tgctgtattt gacgtgcata aaaaacaaca cgatcagcac catttttgtg
     1741 gtggcaacgg ccagcgccag tggggcattc cagactccca ggtcaaaggt ggcaatccag
     1801 acagtgaccc cagtcagaac catcaaggcc ccaaagacac tcagatatgt tttcagcgga
     1861 acgacgtgtc cagccatatt ttttcctcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnna ttcttcattc cttaatgtcc gccgcccttg tagtgcaatc cggtcaagta
     2041 caggagcggg aacaggaaca cccacacgat gtcaacaaag tgccagtaca atccagccat
     2101 ttccaccggt gtgctgtact caggtgaaaa cgtgtctttc caggacatat acaagatggt
     2161 ggcgagaaga cccatcccca cgaccatgtg gagggcgtga attccagtca tacaaaagta
     2221 caggaagtgg aagaccggtt cggtggcgtc aaccccacca tttggcaacg agttataaac
     2281 gaaatttaac cctggcactt tgttgtggtg atagtgctca gtgtattcaa tcgccttaat
     2341 ccccataaag gcggcgccga ggatcatggt caaaaccaga aatcccatca acttgccttt
     2401 gtggccttcc tgggccgcat gcacagccag cgccatcgtt aaactgctgc agatcagcac
     2461 gatggtattg gtcaatccaa gtttgattcc cagttcataa ctgccaatca caaacgtgtc
     2521 gtggtgatag tatcgaaaca gggaatatgc caggaataac ccaccaaaca ggagaacttc
     2581 agtggccaga aacgaccaca tccccaatgt ggctgattca tgctgctgct cagcgtcagc
     2641 aaagtggtgt aagaccgcaa gtgaatttga atgagttgac acggtttagt ctcctgtggc
     2701 accagtttca gatggcattc gatatccagg cattggatcc tgacctggag caagctgtgg
     2761 atcttcgccc attggatgca atggatagtt gtatgcatct tcggtcacaa ctggggtctc
     2821 agcaaaatta tcccagggtg gcggggaggc gactgtccat tctaacccct ttgcgttcca
     2881 aggattatca accaccggac cgtatttcaa cgaccagaac agataaaaca gtggcaggat
     2941 atacccaatc gccagaatgg tcgagccggc ggttgagagc acgttccata cctgaaattc
     3001 cggagcatag atgtaatacc ggcggggcat tccgagatag cccaccacaa actgtgggaa
     3061 gaaggtcaga ataaacccaa tgaacacaat caaggcactc aaccgacccc acccttccgg
     3121 atacattcgg ccaaacatct tgggccacca gtaatggaga ccgccaaaat aggccatcac
     3181 cgcaccgccg accatcacaa aatggaagtg ggcaatcaca aagtaggtat cgtgcaggtg
     3241 ttgatccaat cccatcgagg ccaaaaacaa cccggtcaaa ccaccaatga taaataaccc
     3301 aataaaccca agcgcataca gcattggggt atcaaaggaa attgatcctt tatagagcgt
     3361 cgccgtccag ttgaagacct tcacgcccga ggggatggnn nnnnnnnncc gccacaaagt
     3421 aggtgataaa cgaaaagacc attccggcat agaccgacat ggtggacaca aacatatggt
     3481 gtccccacac cagaaagcca aagatggcga tcatcattga cgacacggca ataaatttgt
     3541 acccaaaggg ttttttgcga gcaaaggctg cgatcagttc gctcaccaca cccagactcg
     3601 gcaaaatcat gatatagacc gctggatggg aatagaacca gaacatgtgc tggaacaata
     3661 ccgggtcacc gcccagggcc ggatcaaaaa tcccaatacc agcaaagcgt tcagcggcca
     3721 gcaagaccat cgagatcgcc agaaccggcg tggccagaat ctggatcagg ctggtggcat
     3781 agagcgccca cacaaagagc ggcatccgga accacgtcaa tcctggtgcc cgcatcttgt
     3841 gggtggtcac aataaagttg agtccggtca gaatggacga gaatccaagg acaaaaaccc
     3901 caagcgtcgc cgcaatcacg ttggtgttgc tgtatttggt gctatagggt gtatagagcg
     3961 tccagcccgt gtctaacccg ccaaccataa tgatccacag gacatagatc gccccaacaa
     4021 taaagatata ccagctcagc aggttgatct tgggaaaggc cagatccttg gccccgatca
     4081 tcagtggcag gagaaagttc ccgagcgttg ccggaattga cggaatcagg aagaaaaaga
     4141 tcatcaccac gccgtgcatg gtgaaaatct tgttataggt atcacctttt aaaaactctc
     4201 cgtggggcgt aagcaattgc aaccggatca ggaaggcaaa gaaaccgccc aggaagaaca
     4261 tcagggtgat ggaaaccaga tacagcaggc cgatgcgctt gtggtcggtc gtgagcaacc
     4321 acgatttcca gccaaagcct tcatttaaat aatgtttttc aggaaatttc actgtcgcag
     4381 caggtgtcat agtgattcct cacacctcat tccttcttag atggttgtgt ctctgacgac
     4441 tggggggctt tgggagcttc agtagcgctg gctggggctt tcggggcagg aacacccgtc
//
LOCUS       DAOQDO010000183         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13009_length_4496_cov_2.336861, whole genome shotgun sequence.
ACCESSION   DAOQDO010000183 DAOQDO010000000
VERSION     DAOQDO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13009_length_4496_cov_2.336861"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /locus_tag="PKZ53_04130"
     CDS             <1..2100
                     /locus_tag="PKZ53_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ39655.1"
                     /translation="AGLAAALHAKRQGLRYVVLEGVRIANTIVNFPKGKPIFAEPMQI
                     ANPSELPVVESTKEETLATWFQLLDREQLSILEGMNVRDVRKNQDGVFEVTVEDKPAV
                     KARFIVLATGKAGKSRTLNVPGEKLPKVSDRLFSPLDYRDQNILVVGGGDSAIETAIA
                     LAETGNNVTISYRKGDFSRIKEGNARQLETLFQAGKVTVLYHSTVKEITETSVVLQIG
                     KETRRLPNDVVFTMIGKELPYEFLERLGITIENTWTAARFALFGLSVFVFSMVYFGKS
                     VAGKLTPAASLGAKIGVLVIPGLTVIAMMATVLYVYWTTNRHKLTNFQSLVPPLLTAV
                     VTTGITLLSLFLVCDHVGPDGKPAPYKLLGLDQYFWYSALYSLAILVFGIRRIVTRKN
                     DYITRQTISLIFFQVVMLFGLPYGLEFGVKGGFLHLPNWMETYVFPNQSYSNIYGLVL
                     AYPLYIHNVLTGEPSAFWLGMSILQTFVIIPALIYYFGKGAYCGWICSCGALAETLGD
                     DYRMLAPHGEKAKRWENLGQVILAAIVLITILWALGHWTPEGSVFRTARLGSLTLAKL
                     SLGLKNLYSLVVDVAFAGAIPLAVYFFYSGRIWCRYGCPLAALMHIYTKFSRYRIFSD
                     KKKCISCNICTKVCHMGIDVMGYASQGRPMDDVECVRCSACVVSCPMDVLSFGQTGTG
                     HPHDPGPNLVQLRRVRS"
     gene            2527..3678
                     /locus_tag="PKZ53_04135"
     CDS             2527..3678
                     /locus_tag="PKZ53_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dispase autolysis-inducing protein"
                     /protein_id="HNJ39656.1"
                     /translation="MKVKLFVVLAVFLISLSVKSGLAIPFDSTVSGQELPSLRPPCGV
                     VQGTGAITMTMDNGASLLPTSENLRGIGYTYGLTALRTPGTLLAVHNATILRSTNNGC
                     SWASIGDVDTSLGDYFPLKLVAGAGDRAFGWSENRTFLIRIDGDRFTYLKAPVEGIIG
                     IGTDPDNADHVRIGDSSGILESFDGGDNWQLIALLPKKDGAVFPYRVAFAPENLNHIF
                     VGTVVKGAFVTTDGGRNWQQATGFNGKDKPANVFEFAISPVNDNIVWAMGINLTEANQ
                     GTPSGGRHIYLSANGGQTFYPVIDHSANVTIRNQPAMAAHPTNPGILYFVFGTYYQDY
                     GTDVFRYNLRDQSLTKTHNSYDGIDSITFSRKDPSIMYFGLEVVEPVGP"
     gene            complement(3792..>4496)
                     /locus_tag="PKZ53_04140"
     CDS             complement(3792..>4496)
                     /locus_tag="PKZ53_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HNJ39657.1"
                     /translation="GQGKASDGKSLYFNREITDRDLEGRTLRELALIRNTIFARAGNP
                     FRKQWLHDYFTAQTWYKPLERMDDSKLTELDRRNASFVADYEAGISKSDLAAQKARLL
                     EKSGKTILSEEERIELSLLARSLGEAPTDEAGAASPLDNPKLLDKLLRVDQLKDYSLR
                     DLQLLRNTIYARRGRGFKRESIREYFSRMEWYREDPAYTDAMLNETDKRNIQIILSVE
                     QELGGPLQGDNWFGGA"
BASE COUNT         1014 a         1177 c         1128 g         1177 t
ORIGIN      
        1 gcgggattgg ctgccgccct ccacgccaaa cgccaggggc tgcgatatgt ggtgctcgaa
       61 ggcgttcgga ttgccaatac aattgtgaat ttccccaaag gcaagcccat ctttgccgaa
      121 cccatgcaga ttgccaatcc cagtgaactt cccgtggtcg aatcaacgaa agaagaaacc
      181 ctggccacct ggttccaact ccttgaccgc gagcaactca gcattctgga agggatgaat
      241 gtgcgcgatg ttcgcaaaaa ccaggatggt gtttttgaag tcaccgtcga ggacaaaccc
      301 gccgtgaaag ccagatttat cgtgctggca accggaaagg ctgggaaatc acgcacgctc
      361 aatgttccag gtgaaaaact gccgaaagtt tctgaccggt tgtttagtcc gcttgattac
      421 cgtgatcaaa atatcctggt cgttggcggt ggtgattccg ccattgaaac cgcgattgcc
      481 ctggctgaaa ccggaaacaa cgtcacgatt tcttaccgca aaggggattt ttcgcgcatt
      541 aaagaaggca atgcccggca actggaaaca ctttttcagg ctgggaaagt cacggtactc
      601 tatcactcga cagtcaagga aatcactgaa acctcagtgg tgcttcaaat tggcaaagaa
      661 acccgacgcc ttcccaacga cgtggtgttc accatgattg gcaaggaact gccctatgag
      721 ttcctcgaac gactcggcat cacgattgaa aacacctgga cggcggctcg cttcgcgctc
      781 tttggccttt cggtgtttgt cttttcaatg gtctattttg gcaagagtgt cgccgggaaa
      841 ctcacaccag cggcttcgct cggtgccaaa atcggagtgc tggtgattcc gggtctgacc
      901 gtcatcgcca tgatggcgac ggttttgtat gtttactgga cgacaaaccg ccataaactc
      961 accaattttc aaagccttgt acctcccctg ctcacggcag ttgtaaccac gggcatcaca
     1021 ctattgtcgt tatttctggt ctgtgaccat gttgggccag atggcaaacc agcgccgtat
     1081 aagttgcttg gactcgatca atatttctgg tacagcgcgc tctatagcct ggcgattctg
     1141 gtgtttggta tccgccggat tgtcaccagg aaaaatgact acatcaccag acaaaccatt
     1201 tcactcatct tctttcaggt ggtgatgctc tttgggcttc cctacggact ggaatttggc
     1261 gtcaaaggcg gctttcttca cctgccaaac tggatggaaa cctatgtttt ccccaaccag
     1321 tcgtattcaa acatctatgg gctggtcctg gcctatccgc tctacattca taatgttctg
     1381 acgggtgagc cgagcgcgtt ctggcttgga atgagtattt tgcagacgtt tgtcatcatc
     1441 ccggcgctga tttattactt tggaaaaggg gcgtattgcg gctggatttg ttcctgcgga
     1501 gcgctggccg aaaccctggg cgatgactat cggatgctgg cgccacacgg tgagaaagcg
     1561 aaacgctggg aaaatctcgg acaggtaatt ctggctgcga ttgtgctgat caccattttg
     1621 tgggcgcttg gccactggac accggaaggt tcggtcttcc gtacggccag actcggcagc
     1681 ctgacgctcg ccaagctcag ccttggactc aagaacctgt attcacttgt ggtggacgtg
     1741 gcgtttgcag gggcaattcc actggcggtt tacttctttt attcaggccg catctggtgt
     1801 cgctatggct gcccgctggc tgcattgatg cacatttaca ccaaattctc gcgctaccga
     1861 attttttctg acaagaagaa atgcattagc tgcaacattt gcaccaaagt ttgccatatg
     1921 ggaattgatg tcatgggcta tgccagccag ggccgtccaa tggatgacgt cgaatgtgtc
     1981 cgctgctcag cctgcgtggt gagttgtccg atggatgttt taagctttgg acaaaccgga
     2041 accgggcatc cgcacgatcc agggccaaac ctggtacaac tccggcgggt gcgttcctga
     2101 tggcaccatc agcgctgtac cagaatcaat cgttcagaaa accgatagcc agccaggaaa
     2161 agctcaggag aaatcctggg ctttcttttt tgctcgctga aaactcgact ctccaaccca
     2221 gagaacctca ccctgacaat ttcgtaccag cattccacct tcacctctca cctggattct
     2281 ctgggaagag aggtgtttgt ttcaaggaac gtccattgca aacctgtgat atgggtgaga
     2341 tcagcctctc tggcccccgc acctcaccaa tcgctcaggc ctgcaacatt ttctggtcgt
     2401 tccatttctg tccagattca aacagctttc ttctgtttga tgaggaattg ggttgggaat
     2461 acaggttttt ttcaaatcct tcagtccaac ccgtcacact tttttgtttt gttggagtta
     2521 aaaaaaatga aagtaaaatt gtttgttgta ctggcagttt ttctgatttc cttgagtgtg
     2581 aaatcagggc tggccatacc gtttgacagt acggtctcag ggcaggaact tcccagtctg
     2641 cgtcccccct gcggagtcgt tcaaggtacg ggtgccatca ctatgaccat ggataatggc
     2701 gcttcactcc ttccgacctc cgaaaatctg cgtggaattg ggtataccta cggcctgaca
     2761 gcacttcgaa caccgggtac gctgctcgcc gtccacaatg ccaccatcct gcgctcaacc
     2821 aataatggat gctcgtgggc ttcaattggc gacgtggata cctcgttggg tgactatttt
     2881 cccttaaagc tggtggctgg tgccggtgac cgggcatttg ggtggtcaga aaaccggacg
     2941 tttctgatcc gcattgacgg tgatcggttt acctatttga aagccccagt tgaaggaatc
     3001 attggaattg gaacggaccc cgacaatgct gatcatgtcc gcattgggga tagttctgga
     3061 attctggaat cgtttgacgg tggggataac tggcaactga ttgccctgtt acccaaaaaa
     3121 gacggcgcgg tctttcccta ccgggttgcc ttcgctcctg agaatctgaa ccacattttc
     3181 gtcggaacag ttgtgaaagg ggcctttgtc accaccgatg ggggccgaaa ctggcaacag
     3241 gcaaccggat ttaatggaaa agacaagccc gccaatgtct ttgaatttgc catttctccg
     3301 gttaatgaca atattgtctg ggcgatgggc attaatttga ccgaagccaa tcagggtaca
     3361 ccatctgggg ggcggcatat ttatctgtct gccaatggtg gtcagacctt ctatccggtg
     3421 attgatcact cagccaatgt caccattcgg aaccagcccg ccatggccgc acacccaacc
     3481 aatccaggta ttttgtattt tgtgtttgga acctattacc aggattatgg aaccgatgtg
     3541 tttcgttata atcttcgtga tcagtcactg accaaaacac acaattccta tgatggaatt
     3601 gattccatca ctttttcgcg caaagacccg tccattatgt actttggact ggaagtagtt
     3661 gagcccgtag gcccgtaagc ccctgactcc actgtgtttc cccaaccgct ggacattgct
     3721 tcagcggttg ggccattttt ttctccacca agagccggtt tccccaaact ggggaatcaa
     3781 ttccagcttt atcaagcacc gccaaaccag ttatcccctt gcaacggccc accaagttcc
     3841 tgttcgacgc tgagaataat ctgaatattg cgtttgtcgg tttcattcag catggcgtcg
     3901 gtgtaggccg ggtcttcgcg ataccattcc atccggctaa agtattcgcg gattgactca
     3961 cgcttaaagc cacgtccccg ccgggcataa attgtgttgc gcagcaattg cagatcccgc
     4021 aatgaataat ctttcaattg atctaccctg agcagcttgt cgaggagttt tggattgtcg
     4081 agcggcgacg ccgccccggc ttcgtccgtc ggcgcttcgc cgagcgaacg ggccagcagc
     4141 gaaagttcaa tgcgctcttc ttcgctcaaa atggttttgc ctgacttttc gaggagtctg
     4201 gctttctgag ccgcgagatc agattttgaa atcccggctt catagtcggc aacaaatgac
     4261 gcgttgcgcc gatcaagctc agtcagcttg ctgtcatcca ttcgttcaag cggtttgtac
     4321 cacgtctggg cagtgaaata atcgtgtaac cactgttttc ggaatgggtt cccggcacgg
     4381 gcaaaaattg tattgcggat taacgccagc tctctgaggg tccggccttc cagatcacgg
     4441 tcggtgattt cacggttgaa gtagagtgat ttcccatcac ttgcctttcc ctgccc
//
LOCUS       DAOQDO010000184         4493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13019_length_4493_cov_3.621451, whole genome shotgun sequence.
ACCESSION   DAOQDO010000184 DAOQDO010000000
VERSION     DAOQDO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13019_length_4493_cov_3.621451"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKZ53_04145"
     CDS             complement(<1..307)
                     /locus_tag="PKZ53_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HNJ39658.1"
                     /translation="MENYVEVAAELERRVVRWMARYGIVLLRVSLGIVFFWFGILKFF
                     PNMSPAQDLALRTIHVLTLGMLPGWLTLWVLASWECTIGVGLVAHEVLERLHHVIVVE
                     "
     gene            complement(487..804)
                     /locus_tag="PKZ53_04150"
     CDS             complement(487..804)
                     /locus_tag="PKZ53_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HNJ39659.1"
                     /translation="MKVTIKERTTGTVLASTDDPSKVESFEGNWYFDPSVVNQVELQV
                     TDDLYTCPYKGTCNWVSFSGQGKTAMRIAWVYPNPKPGFENIKGRFAFYGGNRSSTIE
                     EVL"
     gene            complement(900..2246)
                     /gene="thiC"
                     /locus_tag="PKZ53_04155"
     CDS             complement(900..2246)
                     /gene="thiC"
                     /locus_tag="PKZ53_04155"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNJ39660.1"
                     /translation="MLACEGKITEDMIMVAEDERLEPEFIRQGLANGTIVIPKNINHD
                     FRPIGIGKGLRTKVNANIGASGYHQFVEEEIEKLHVSVHYGADSVMDLSTGTDLDLIR
                     ETLISRSPLMLGTVPIYQVASEGSILKMDPEELFAVIEKQAQQGVDYMTVHCGVTKET
                     VKKLRGHQRIEGIVSRGGSLLAAYIEATGKENPLYEQFDRLCDIFARYDVTFSLGDGL
                     RPGATGDASDRGQLAELLVLGELTARARAKGCQVMVEGPGHVPLDQVQANVQLQKKVC
                     EGAPFYVLGPLTCDVAPGYDHITGAIGGAIAAAAGTDMLCYVTPAEHLRLPDHQDVIE
                     GIIATRIAAHSGDLVKGVKGAKEWNDQMSLYRKRLDWEGMYRLAMDPEKARRYKEESE
                     AAGSKVCSMCGSLCSINIDNAAIKKFSGSVPDTSEKFLTKKPEVPEAVVAEAVAGD"
     gene            complement(2466..3872)
                     /locus_tag="PKZ53_04160"
     CDS             complement(2466..3872)
                     /locus_tag="PKZ53_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNJ39661.1"
                     /translation="MDREIKELEKALEQIWDIALKFGLDPFPVHFEIVPATVMYEIGS
                     YALPGRYSHWTFGKAYHRMKMMYDLGLSRIYEVVINTNPSYGFLLETNSLTQNKLVIA
                     HVLGHVDFFKNNAYFSRTNRRMVDEVGVHSSRINEYEFTYGRKAVEEFLDAVLSIEEH
                     VDPDFLIKKARNQTPAERQKKSSRQGKYDDMFTDQELDRKTEAPKIYVPGEPVLPEKD
                     LVWFIAQYSPILEPWQRDIMSMVHEEMLYFVPQIQTKTLNEGWASLWHARIMRELGLE
                     GDEYIEFAELHAGVVSPHKGQLNPYYLGYKILEDIERRWDNPTAEEREKYGRKGGEGR
                     HKLFEVREMENDTSLLRNYLTEQLVEELDLYVYELVDDEEWTITEKRWERVRDQLVAS
                     RTNFGFPYIEVTDADYNRNRELYLTHRFEGTELDLKYGRKVLEYVQKLWGRTVHLETM
                     VDNESLVLHYDGKEHDED"
     gene            complement(4092..>4493)
                     /locus_tag="PKZ53_04165"
     CDS             complement(4092..>4493)
                     /locus_tag="PKZ53_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ39662.1"
                     /translation="ASNNDLNLFEEIDTAAKLHKEFSRNPTVVSAREALEMATIGGAR
                     AIHQDDRIGSLEPGKLADLIVVNLDTPHQWPVYNLYSTLVYATKASDVETSIINGKLV
                     MRDRKVLTLNSVAIRKEALLYRTRILESLKK"
BASE COUNT         1070 a         1151 c         1083 g         1189 t
ORIGIN      
        1 gttcgacgac gatgacgtgg tggaggcgct cgagcacttc gtgagcgacc aaccctacac
       61 caatggtaca ctcccaactc gccagcaccc acaaggtcag ccatccagga agcatcccaa
      121 gggtcaaaac atgaattgtc cgcagtgcta aatcctgtgc ggggctcata ttcgggaaaa
      181 atttcaagat cccaaaccag aaaaaaacga ttcccaggct aacccgcaag agcacaatgc
      241 cataccgtgc catccatcgc acaacccggc gttcaagttc cgccgccact tcaacataat
      301 tttccatcct gaactgagtc tccttcatgt gttgaattgt ctgtgcggca tcggtggttg
      361 ttgataggaa tccaggttgg aatcaagatt gactcatcac caggctgtga aatactttta
      421 tttcaatggt ttaataatca atttagacaa gagagctgct tgagggacgc accctgtatg
      481 cgcctgtcag agcacctctt caatcgtact tgagcgattg ccaccataaa acgcaaaccg
      541 acctttgata ttttcaaaac ctggttttgg gttgggatac acccaggcaa tccgcattgc
      601 tgttttgcct tgccctgaaa aactgaccca gttacaggtg cctttgtatg ggcaagtgta
      661 gaggtcatcg gttacctgca actccacctg attgaccacc gacgggtcaa agtaccagtt
      721 gccttcaaat gattcgactt ttgatggatc atcggtcgaa gccagtacgg tgccggttgt
      781 tcgttctttg atggtcactt tcattgcgga attcctttcg atttgacaaa aaagcccccg
      841 ttggaacggg ggcttttgtt tggattgagt tgagaaaacc cggcttggcc tgatgatact
      901 tagtcgccag ccaccgcttc agccacgact gcctcaggaa cctctggttt tttcgtgagg
      961 aatttctcag atgtatcagg tacgctgcct gagaattttt taatcgcggc gttgtcaatg
     1021 ttgatcgaac acaggctgcc gcacattgaa cagaccttcg agccagccgc ttcggattct
     1081 tccttgtagc gacgagcctt ttctggatcc attgccagcc ggtacatgcc ttcccaatcc
     1141 agccgcttgc gatagagtga catctggtca ttccattctt tggcaccttt gacgcccttg
     1201 accaggtcgc cggaatgggc cgcaatacgg gtggcaatga tgccttcgat gacatcttga
     1261 tgatctggca ggcgcaggtg ttcagcgggt gtcacatagc acagcatatc ggttccagcc
     1321 gcagcggcaa tcgccccacc aatcgccccg gtaatgtggt catagccagg tgccacgtca
     1381 caggtcagcg gcccaaggac atagaacggc gcgccttcgc ataccttctt ttgcaactgg
     1441 acattggcct gaacctgatc aagcggcacg tgtccagggc cttcgaccat cacctgacag
     1501 cctttggccc gcgcacgggc ggttaattca cccaagacca gcagttcggc cagttgtccc
     1561 cggtcagacg catcacccgt ggcaccagga cgcagcccgt caccgagcga gaatgtcacg
     1621 tcatagcggg caaaaatgtc gcacagccgg tcaaattgtt catacagtgg attttctttg
     1681 ccggtggctt cgatatatgc cgccagcagc gagccaccac ggctgacaat gccttcgatg
     1741 cgctggtggc cgcgcaattt tttgactgtt tccttggtga ctccgcagtg aaccgtcatg
     1801 taatccacgc cctgctgggc ctgtttttca atgacggcaa ataattcttc gggatccatc
     1861 ttgaggattg aaccttccga ggccacctgg taaatcggaa ccgttcccaa catcaagggg
     1921 ctgcgcgaaa tcaaggtttc gcggatcaag tccaggtctg ttccagttga aaggtccatc
     1981 acgctatcgg caccatagtg taccgagacg tgcagttttt cgatttcttc ttcaacaaac
     2041 tgatgataac cagaggcacc gatattggcg ttgactttgg ttcgcaagcc ctttccaatg
     2101 ccgatgggcc ggaagtcatg gttgatgttt tttggaatga caatcgtgcc gttggccaga
     2161 ccctggcgaa tgaactctgg ttccagtcgt tcatcctcgg ccaccatgat catatcctcg
     2221 gtgatcttgc cctcgcaggc aagcatgcgc aacgtttgat attcacctct tttcacaaga
     2281 gtgcctccct gttctaaaac taagtgcaat gtgtacaact gtggttgggt agttgggtgt
     2341 cagtgtttgg aaacctgaga actggtttgg tgactggcgc ttgtaaaata ttgtctccta
     2401 agtactaagc actcagtacc agtcaccggg tacaacgtgc tctgcacctt tcacactgac
     2461 gaaaatcaat cttcatcatg ttctttcccg tcatagtgca acacgagact ttcattgtcc
     2521 accatggttt caagatgcac ggtgcggccc cacagctttt ggacatattc gagcaccttg
     2581 cggccatatt ttaaatccag ctcagtgcct tcaaaacgat gggtcaaata gagttcgcgg
     2641 ttgcggttat agtcggcgtc ggtgacttca atgtagggaa acccaaagtt ggttcgactg
     2701 gcaacaagct gatcgcggac ccgttcccaa cgcttctcgg taatggtcca ctcttcgtca
     2761 tccaccagtt cataaacata caaatccagt tcctcgacta attgttcggt caaataattg
     2821 cgcaacagcg acgtgtcgtt ttccatttcg cgaacttcaa agagtttgtg tcgcccttca
     2881 ccgcctttgc gtccatattt ttcgcgttct tcagccgtcg gattgtccca gcggcgttcg
     2941 atgtcttcca ggattttgta gcccaaataa tagggattga gctgtccttt atgcggggaa
     3001 accacaccgg cgtgaagttc agcaaactca atatattcat caccttccag accaagttca
     3061 cgcattatcc gtgcatgcca aaggctggcc caaccttcat ttaatgtttt agtttgtatc
     3121 tgtggcacga agtagagcat ttcctcgtga accatactca tgatgtcgcg ttgccagggt
     3181 tcaagaatgg gcgaatattg ggcaatgaac cagaccagat ctttttcagg cagcactggt
     3241 tcaccgggaa catatatttt gggggcttcg gtttttcggt ccagttcctg atcggtgaac
     3301 atatcgtcat atttgccctg acgggagctt ttcttctggc gttcagccgg agtttggttc
     3361 cgggcttttt tgatcaggaa atcagggtca acgtgctctt caattgacaa aaccgcatcc
     3421 agaaactctt cgacggcctt gcggccatag gtgaattcat actcgttgat gcgtgacgaa
     3481 tgaacgccaa cttcgtcaac catccggcgg ttggtgcgtg agaaataggc gttgttttta
     3541 aagaaatcaa cgtgaccgag cacatgggca atgacgagtt tgttttgggt taaagagttg
     3601 gtttcgagta aaaatccgta ggaaggattt gtattgatga caacctcgta aatacgtgac
     3661 aaaccaaggt catacatcat tttcatccga tgataggctt tcccaaacgt ccagtgcgag
     3721 taccgaccag gcagggcata ggacccgatt tcatacatca ccgtggccgg cacaatctca
     3781 aaatgtactg gaaatggatc aagtccaaac ttcagcgcaa tgtcccaaat ctgttcgagc
     3841 gctttttcaa gttctttgat ttcacgatcc attggggaac ctccttctga agaatgaaga
     3901 atgaagaatg aagaaaatgg gttggtgatg gatgcttcgg aatacacgga acacacagaa
     3961 actaaatcca aagtcttcaa taatttctga gacttcccac actaaaaggt caggaaatat
     4021 ttatctgaaa ggctatacca aatagtttct tcattcttca ttctcaattc ttcattctgc
     4081 tttctttcgc cctatttctt caggctttcc agaatgcgcg tacgatagag cagggcttct
     4141 ttgcgaatgg caacggaatt gagtgtgagc actttgcggt cgcgcatcac gagttttcca
     4201 ttgatgatcg aggtttcgac gtcagacgcc ttggtggcat aaaccagcgt cgaatagaga
     4261 ttatacaccg gccactggtg tggggtatcc agatttacca caatcaggtc ggcgagtttg
     4321 ccgggttcga gcgaaccaat ccggtcgtcc tgatgaatcg cccgggcacc gccaatggtt
     4381 gccatttcca gtgcctcgcg ggctgaaaca acggttgggt tgcgggaaaa ttccttgtgg
     4441 agtttggctg ccgtgtcaat ttcttcaaac aggttgagat cgttgtttga agc
//
LOCUS       DAOQDO010000185         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13063_length_4484_cov_2.834500, whole genome shotgun sequence.
ACCESSION   DAOQDO010000185 DAOQDO010000000
VERSION     DAOQDO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13063_length_4484_cov_2.834500"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2772)
                     /locus_tag="PKZ53_04170"
     CDS             complement(<1..2772)
                     /locus_tag="PKZ53_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ39663.1"
                     /translation="MFVPPPPVQESTSLSICLVCNTNTIPSTVVTQLAAIGEVVTHTF
                     ESNQSGSQTCVYVYWVQTPDDLRHCGRTIGKRSTRSVVVVPYGLADLATQLYENHPGL
                     ALVWDDRLQPLIPVVRRLAVPLRQVSSLPTPDYLRPISTPLPNLQESRLKALTSVIPD
                     TWIRISREGKYLDFGAPSPELLALSPETLIGSTIDDLPIPPELKQQFKEVIDQAFQTR
                     HLQTLEYDMEVPAGQKCFELRALASSDTEAILIVRDQTDARHSAETIRISEERLRFAL
                     EGAQIGTWHWDIVTNDMEWSDRYYDIFGLDRTLAPSLGLWEGLLHPDDHDRVIQMLGE
                     MIERQNESSAEYRILRPDGSVRWVNVVGRISRQPDGTPLQMEGIIFDITDRQLAQEKI
                     RQSEEWFRTLVQHSSDIVAVIQPDGIRRFVSSGARRVLGYAPDDLIGKNAFQGVHPED
                     MARVEQTFRTVLTEPTQTVQIEFRFQHLNGHWVDLELIGENYLEIGGVEGVVVNIRDI
                     TARKKAQDLLLRSEEKFAQIFHDSPMAICIISLQTAEILDVNQAFLDISDYSRQDLVR
                     RTPAEVGLWRSIRGLERLREKLLASDGLLSFTAELRRRTGEIRIAEGRSTLMELPEER
                     CALTIFVDATERFRMEQALRVNQERLTETGRLARVGGWEVNLQAEQVYWTEEVFLIHD
                     MQPGRQPTRDEAFGFYPPDAQTVLHLAVEQTIQTGVPWELELPLITATGRQIWVHSLG
                     HVEYRDAEPYRLHGAVQDVTDRHLREERMRLLESAIEHANDAVFITEAEPVAPPGPPI
                     VYVNQAFVNVTGFRQSEVIGLTPTILKSPRAKPDELDRLIEAIQRHEPITFEHIISRK
                     DGSEFWGEISIFLVIDVTGNHTHWVAILRDITERHVIEDRLRQSQRMETLGQFSSGVA
                     HNFNNLLTVI"
     gene            complement(2929..>4484)
                     /locus_tag="PKZ53_04175"
     CDS             complement(2929..>4484)
                     /locus_tag="PKZ53_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39664.1"
                     /translation="IKKQFGFVPDPIKIELKRLSPPQLESVIDILPQFASVKYLTTHI
                     QELPQMGITPGDRGTPREPVRSEYAPQPEGFSDSVETAHAYQPPAHAIPVFAEPAEPE
                     AEPVQSSFAAARFERITDDDTAVVSEPVELPSSSEPAPVSSSRPVVSSPPVQPRPQPY
                     TPLPPATKSMTNQPHYRSDQYSRSDVVRSTLQVTLDTKTINLVFEFDKYSDAENQRGI
                     RFLLSIDENELIFAQFLAQRNRIEPLEARVAAAIQAYFRDAEKVLSGAVAAQAQPSPV
                     VAPAPVSAPAPIVESPAPAPIPVPVVAATPVEVLTPVTDTDDADAEAGTDDLSTSEKP
                     RRHRRTNAELQAAGVKVEKRTRLTPQSIDLNTLKFDPRMASILSFDEAKALNIAAVGF
                     IPNSTDVLTLFVEPEGKVRIYTFFNRVTNRAALAALIGIPSKDREKVKIRTLRVPQEF
                     LDEFLADGAAAVVPTAKPTPIVITEESDDPSKPPRRRFIVPKEHAGRPTPSEELPKRK
                     RGRPAGSGK"
BASE COUNT         1029 a         1160 c         1235 g         1060 t
ORIGIN      
        1 aataaccgtt aacagattgt tgaagttatg ggccacgccg ctggaaaatt gccccagcgt
       61 ttccatccgt tgcgactgcc ggaggcgatc ttcgattacg tgccgctcgg taatatcccg
      121 cagaatcgcc acccagtggg tgtggttgcc tgtgacatca atcaccagga aaattgaaat
      181 ttccccccaa aactctgaac catctttgcg agagatgatg tgttcaaaag taattggctc
      241 gtggcgttgg atcgcctcga tcaagcgatc cagttcgtca ggtttggcac gggggctttt
      301 caaaatggtc ggcgtcaggc caatgacttc agattgcctg aagccggtta cattcacaaa
      361 ggcctggttg acatacacaa tcggggggcc tggaggggca accggctccg cttcggtgat
      421 aaacaccgca tcgttggcat gttcaatggc tgattcgagc aaccgcatcc gttcttcacg
      481 caggtggcgg tcggttacat cctgaacggc cccgtggagc cgatagggtt cggcgtcgcg
      541 atattcaaca tgccccagag agtgaaccca aatctgccgc ccagtcgcgg taattaacgg
      601 caactcaagc tcccagggaa ccccggtttg aatcgtctgc tcgactgcca gatgcagcac
      661 cgtctgggca tcaggcgggt aaaacccaaa ggcttcgtct ctggtcggtt gacgtccagg
      721 ctgcatatcg tgaatgagaa agacttcttc cgtccagtat acctgttcgg cctggagatt
      781 gacttcccac ccaccgacgc gggctaaacg cccggtctca gtcagtcgct cttgattgac
      841 ccggagggcc tgttccatcc gaaaccgctc ggtagcatcc acaaaaatgg tcagggcaca
      901 gcgttcttca ggtaattcca tcaaggttga ccgcccttct gcaatgcgga tctcaccggt
      961 gcgacggcgc aattcagccg taaacgataa caacccatcc gaagcgagca gtttctcccg
     1021 caatcgctct aacccccgaa ttgatcgcca gagcccaacc tcagccggtg tgcggcgaac
     1081 cagatcctgg cgtgaatagt cggaaatatc caaaaaagcc tgatttacat ccaggatttc
     1141 cgctgtctgg agcgaaataa tacaaatggc catggggcta tcgtggaaaa tctgggcaaa
     1201 cttttcttcg ctccgtaaca gtaagtcctg ggcttttttg cgggcggtaa tgtcacgaat
     1261 attgaccaca accccttcga ctccaccaat ttccagatag ttttcaccga ttaattccag
     1321 gtctacccaa tggccgttca gatgctggaa cctgaattca atttggacgg tttgggtcgg
     1381 ttcagttaaa accgttcgaa aggtttgctc tacccgggcc atatcttcgg gatgcacgcc
     1441 ttgaaacgca ttcttgccaa tcaggtcgtc gggcgcatac cccagaacgc gtcgggcgcc
     1501 agagctgaca aaccgccgta tgccatccgg ttgaatcacg gccacaatat cggaggaatg
     1561 ctggaccaga gttcggaacc attcttcact ctggcggatc ttctcctggg ccaactggcg
     1621 atccgtgatg tcaaaaatga tcccttccat ttgtaatggg gttccatcgg gctggcgact
     1681 gatacggcca acgacattca cccaccgaac tgaaccgtcc ggtctgagga ttcgatattc
     1741 ggcgctgctt tcattttgtc gttcgatcat ttcaccgagc atctgaatca cccggtcgtg
     1801 gtcatctgga tgcaacagtc cttcccaaag ccccaggctg ggtgccagag ttcggtccag
     1861 gccaaagatg tcataatacc gatctgacca ttccatgtca ttggtgacaa tatcccagtg
     1921 ccaggtaccg atttgggctc cttccagagc aaagcgaagc cgctcttcgc tgattcggat
     1981 ggtctctgcc gaatgccggg cgtcggtctg gtcgcgcaca attaaaatcg cttcggtgtc
     2041 gctgctggcc agagcgcgaa gttcaaagca tttttgaccc gcaggaactt ccatgtcata
     2101 ttccagggtt tggaggtgcc gggtttgaaa tgcctgatca atcacctctt taaattgctg
     2161 tttgagttcg ggcgggatcg gaaggtcgtc aatggtggac ccaatcagtg tttcggggga
     2221 gagggccagc agttcgggcg aaggcgcccc aaaatcaagg tatttccctt cacgactaat
     2281 tcgaatccag gtatctggaa tgacactggt cagggctttt aatctggatt cctgcagatt
     2341 gggaagcggg gtactgattg gtcgcaggta atcgggggtt ggtaaagacg agacctggcg
     2401 caaggggaca gccagtcggc ggacaactgg tatcaggggc tgtaagcgat catcccagac
     2461 cagtgccagc cccggatggt tttcatagag ctgggttgcc aaatctgcca gaccatatgg
     2521 gacaaccacc actgaccgcg tcgaccgttt tccaatggtc cgcccacagt ggcggaggtc
     2581 gtcaggggtt tgaacccagt agacgtagac acaggtctgg cttccggact gatttgattc
     2641 aaaggtatgt gtgacgacct ctccaatcgc ggcaagctgg gttacaaccg tggagggaat
     2701 tgtattggta ttacaaacta gacaaatcga aagactggtg ctttcctgga caggaggagg
     2761 gggaacaaac atcgtttgtg tcagccaaag tgaagggtgg ctcaagaacc tgggtaaacc
     2821 aaacgggaag gtttgcgaaa catacaggct gacagataac tctcagccag ttcctgtttc
     2881 gcagaacttc ccgttctttt tttccaattg aacagcgact ctcgcaactt actttccaga
     2941 gcccgccggg cgaccgcgtt tccgcttggg caactcttcg ctcggtgtcg ggcgaccagc
     3001 gtgttctttg ggaacaatga atcgccgacg ggggggtttg gatggatcgt cagattcttc
     3061 ggtaatgaca atcggggttg gtttagcggt tggcaccacg gcggcggcac catcggccag
     3121 aaattcgtcg agaaattcct gtggaactcg caacgtgcgg attttgacct tttcacggtc
     3181 tttggatgga attccaatca aagccgccag ggccgcccgg ttggtgaccc ggttgaagaa
     3241 tgtgtaaatc cgtactttgc cctcaggctc aacaaacagg gtcagcacat cagttgaatt
     3301 tggaataaac ccaactgccg cgatattgag tgctttggct tcgtcaaaac tcaaaataga
     3361 agccatgcga ggatcaaatt tcagtgtatt taaatcaata ctttgtggcg ttagccgggt
     3421 acgcttttca accttgaccc ctgccgcctg gagttccgca ttggttcgcc gatggcgacg
     3481 gggtttctca gaggttgaca gatcatcggt cccggcttca gcgtccgcat catcagtatc
     3541 agtcaccgga gtcagtacct cgaccggagt cgccgccaca actggcactg ggattggcgc
     3601 cggagctggc gactcaacga ttggcgctgg ggctgaaacc ggcgccgggg caacgacagg
     3661 agacggctgg gcctgagccg caacggcacc tgacaacact ttctcggcat ctctgaagta
     3721 tgcctgaatc gccgccgcaa cccgcgcttc aagcggctca atccgatttc gttgggccag
     3781 gaactgggca aaaatcaact cgttttcgtc aatagacaac aggaatcgaa tgcctcgttg
     3841 attttcagca tctgaatatt tgtcaaattc aaaaaccagg ttgattgttt tggtgtccag
     3901 ggtcacttgc agcgtggagc gtaccacatc cgaacgggaa tactggtctg agcgatagtg
     3961 tggttgattg gtcatagatt ttgtggctgg cgggagaggc gtatacggct ggggccgggg
     4021 ttgaaccgga ggggatgaaa caaccggtcg gctggaactg accggtgcgg gttctgaact
     4081 cgatggcaat tcaaccggtt cactcaccac ggcggtatcg tcatcagtaa tgcgttcaaa
     4141 ccgggcggcg gcaaacgatg attgaactgg ttcggcttcc ggctctgctg gttcggcaaa
     4201 aactgggatt gcgtgtgcag gtggctgata ggcgtgagct gtctcaaccg aatcggaaaa
     4261 cccttcgggt tgaggggcat actctgaccg tactggctca cgaggcgtac cgcgatcacc
     4321 tggggtaata cccatttgtg ggagttcttg aatatgtgtg gttaagtatt ttaccgaggc
     4381 aaattgaggc aaaatatcaa tcaccgattc cagttgaggt ggtgagagtc gtttcagttc
     4441 aattttgatt ggatctggca caaaaccaaa ctgttttttg attg
//
LOCUS       DAOQDO010000186         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13130_length_4470_cov_3.253907, whole genome shotgun sequence.
ACCESSION   DAOQDO010000186 DAOQDO010000000
VERSION     DAOQDO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13130_length_4470_cov_3.253907"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /gene="clpX"
                     /locus_tag="PKZ53_04180"
     CDS             <1..821
                     /gene="clpX"
                     /locus_tag="PKZ53_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNJ39665.1"
                     /translation="DATTLTEAGYVGEDVENIILKLLQAANGDIERAQQGIIYIDEVD
                     KICRKDENPSITRDVSGEGVQQALLKILEGTIANVPPQGGRKHPHQEFYPVDTSNILF
                     ICGGAFVGIEKVVERRVGKKSLGFNAKLEYKRFHRNSQILELTQPEDLIKYGLIPEFV
                     GRLPVMGTLHELDEAAMVDILTKPKNALIKQYQKQFEWDNVKLRFTEEALQTIARMAC
                     ERKVGARGLRMILEELMLDLMYSLPSQKKIKEFTITREMVETRRITLPLLEKAG"
     gene            981..3410
                     /gene="lon"
                     /locus_tag="PKZ53_04185"
     CDS             981..3410
                     /gene="lon"
                     /locus_tag="PKZ53_04185"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNJ39666.1"
                     /translation="MEEYHDSTPEDVALYPMVPIRDVVVFPHTVVRFKIGRPPSVNAM
                     VQALKGDRIIFLATQHDATIEEPSMEQVFKIGTLARISHHLQLADGNIKVQFEGLERA
                     KSVRFEEHPDGYWVATVRRSPALVDRSPRVNALVSKLTSLVDQYARQNPENPENLHAD
                     LQIEDPSRLADAIANHLKISVEEKQSVLELFSLSDRLNRLIEILDIELEKIQVDRSVQ
                     NRVKKQMERAQKEYYLNEKIKAINKELGRKDEKAELEELKKKIDSAGMSTDAYNKAMT
                     ELRRLEQMPPMSAESAVSRNYLEWLLAVPWKERSEEVTDLKFAEEVLESDHYGLEKVK
                     ERILEFLAVRQLIENPKGSILCFVGPPGVGKTSLGRSIARATGRKFVRLSLGGVRDEA
                     EIRGHRRTYIGALPGQIIQMMKKAGTVNPLILLDEIDKLGMDFRGDPSSALLEVLDPE
                     QNNSFQDHYLDVEYDLSKVMFIGTANVLHTIPAALQDRLEIIRLSGYTEREKIEIAKR
                     YLVPKQREATGLKSDMIDFTEGAITTVIENYTREAGVRSLERELSSVCRKVARKVVVT
                     SEKEAFHETVSSEAILNYLGPIRFRSQMIEEESEVGLATGLAWTEVGGEVLHIETTLM
                     PGRGQLTLTGKLGEVMQESARAAISYVRSRANQLGISPNFYKRYDMHIHIPEGAIPKD
                     GPSAGITMATSIVSILTDTPIRKDVAMTGEITLRGKVLPIGGLKEKLLAAHRIGIRTV
                     IIPRENQKDLVEVPKEILELLTIHLVDHVDQVLRLALKPRKAKNDTSEVSAMWNPSVT
                     ESNQISPPPVE"
     gene            3551..4156
                     /gene="yihA"
                     /locus_tag="PKZ53_04190"
     CDS             3551..4156
                     /gene="yihA"
                     /locus_tag="PKZ53_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNJ39667.1"
                     /translation="MKPVVAQFVCSAVSPHQYPTDRLPEIAFLGRSNVGKSSLINSLL
                     RVKGLARTSSTPGRTQLINFFRINNALYFVDLPGYGYARVPTEIRQQWAPMIEKYLID
                     RAPLALGIVIVDSRLKPMDLDQQMVEWLDAQHVPLCVVATKADKLSNNQMATQLKILR
                     ETFGQAVIPYSALTRKGADEVWRMIRQAGEPKPPAVITATS"
BASE COUNT         1208 a         1171 c         1104 g          987 t
ORIGIN      
        1 ttgacgccac caccctgacc gaagccgggt atgtgggtga agatgtcgaa aacatcatct
       61 tgaaactgtt gcaggcggcc aatggcgaca tcgaacgagc tcaacaaggg atcatctata
      121 ttgacgaagt tgataaaatc tgccgcaaag atgaaaaccc gtccattacc cgcgatgtct
      181 cgggcgaagg ggttcagcag gcactgttga aaatcttgga aggcaccatt gccaatgttc
      241 caccccaggg tggacgcaaa cacccacatc aggaatttta tccggttgac acgtcaaaca
      301 tcctgtttat ttgcggtggg gcgtttgtgg gcattgaaaa agtggtcgaa cgccgggttg
      361 ggaaaaaatc attgggcttt aacgccaagc tggaatacaa acggtttcat cgcaactctc
      421 aaattttaga actcacacag cccgaagacc tgatcaaata cggattgatt ccggaatttg
      481 tcggacgcct tccggtcatg ggcacgctcc acgaactgga tgaagccgcg atggttgata
      541 ttctgaccaa acccaagaat gcgctgatca agcagtatca aaagcagttt gaatgggata
      601 acgtcaagct ccggttcacc gaagaagcgc tgcaaaccat tgcccgcatg gcctgtgaac
      661 gcaaagtcgg cgcccgtgga ttgcgcatga ttcttgaaga attgatgctc gacctgatgt
      721 acagcctgcc aagccagaaa aaaatcaagg aatttaccat cacccgtgaa atggtggaaa
      781 cccggcgcat taccctacct ttgcttgaaa aagccggata aaagggtcgc gctcacccgt
      841 gccgactggt acatatcaat caagcactta cttgctttct ttgatagatc cattcaggcc
      901 aaacaccagc cggtctcaat cgcaacgcgt gttttgcctg agtggaagga ttatgagaaa
      961 aaaaattgat agagagattt atggaagaat atcatgacag tacgccggaa gatgtggctc
     1021 tgtaccctat ggtaccaatt cgggatgtgg tggttttccc gcatacagtc gtccggttca
     1081 agattggtcg gccaccttca gtcaacgcga tggtgcaggc cctgaaaggg gatcgcatta
     1141 tctttctcgc cacccaacac gacgccacca tcgaagaacc cagcatggaa caggtgttca
     1201 agattgggac gctggcgcgg atctcacacc acctgcaact ggctgatggc aatatcaaag
     1261 tccagtttga aggcctcgaa cgggcaaaat cggtccgctt tgaagaacac ccggatggct
     1321 actgggtggc aaccgtcaga cgctcgccgg ctctggttga ccgcagccca cgggtcaacg
     1381 ccctggtgag caaactgaca tccctggtgg atcagtatgc ccggcaaaat ccggaaaacc
     1441 cggaaaacct gcatgccgat cttcaaatcg aggacccgtc tcgcctcgct gatgccattg
     1501 ccaatcacct caaaatcagt gttgaagaaa aacaaagcgt cctcgaactg ttttcactct
     1561 cagaccgact gaaccggttg attgaaatcc tggacatcga actggaaaaa atccaggttg
     1621 atcgttcagt tcaaaatcga gtgaagaaac agatggagcg ggcccaaaaa gagtactacc
     1681 tgaacgaaaa gatcaaggcc atcaacaagg aactcgggcg caaggacgaa aaagccgaac
     1741 tcgaagaact caaaaagaaa attgattcgg ctggcatgag caccgacgcc tacaacaaag
     1801 ccatgacgga actgcgtcgg cttgagcaaa tgcctccgat gtcggctgaa tccgcggtgt
     1861 cgcgcaatta cctggaatgg ctgctggcgg tcccctggaa agagcgctct gaagaagtca
     1921 ccgatctcaa atttgccgag gaagtccttg aaagtgacca ttatgggctg gaaaaggtca
     1981 aagaacgcat cctggaattt ctggccgttc gtcagttgat tgaaaatccc aaagggtcca
     2041 ttttgtgttt tgtgggtcct ccaggcgttg ggaaaaccag ccttggtcgc tcaattgccc
     2101 gagccacagg ccgcaagttt gtgcgtctgt ccctgggtgg tgtccgggac gaagctgaaa
     2161 tccgcggtca tcgtcgaacc tatatcggcg ccctgcccgg ccagattatc cagatgatga
     2221 aaaaggccgg aaccgtcaac ccattgattc tgcttgacga aattgacaag ctgggtatgg
     2281 atttccgggg cgacccatcc tcagctttgc tcgaagtcct cgacccggaa caaaacaact
     2341 cattccagga tcactatctc gatgtcgaat atgacctgtc aaaggtgatg tttatcggga
     2401 cagccaatgt cctgcatacg attccagccg cattgcagga ccgactcgaa atcatccggt
     2461 tgtcaggcta taccgagcgc gagaagattg aaatcgccaa acgctacctg gtgcccaaac
     2521 aacgtgaagc taccgggtta aagtcagata tgattgactt tactgaaggt gccatcacca
     2581 cggtcattga aaattacacg cgtgaagctg gtgtgcgcag cctcgaacgc gagttatctt
     2641 cggtgtgccg caaggttgct cgcaaagttg tcgtgacatc agaaaaagaa gccttccacg
     2701 aaacggtctc gtccgaagcc attctaaatt atctggggcc gattcggttc cgatcccaga
     2761 tgatcgaaga agaaagcgaa gttgggttgg ccaccgggct ggcctggact gaagtgggcg
     2821 gcgaggtgct ccacattgaa accaccctca tgccgggtcg aggtcagttg accctgaccg
     2881 gaaaactggg tgaggtcatg caggaatcag cccgggcggc catcagttat gtccgttcgc
     2941 gcgccaatca gcttgggatc tcgcccaatt tttacaaacg ctatgacatg cacatccaca
     3001 ttccggaagg cgccattccc aaagatggac catcggctgg aatcacgatg gcgacgtcca
     3061 ttgtatcaat tttgaccgac actcccatcc gcaaggatgt ggccatgact ggtgaaatta
     3121 cgctacgtgg caaggtgctc cccattggtg gcttgaaaga aaagctgctg gcggctcacc
     3181 gaattggcat tcgaaccgtg atcattccgc gtgaaaatca gaaagatctg gttgaagttc
     3241 caaaggaaat cctggaactc ctgaccattc atctggttga tcacgttgat caagtcttgc
     3301 ggctggcctt aaaaccacgc aaagccaaaa atgacacctc cgaagtgtca gctatgtgga
     3361 atcccagtgt gaccgaaagc aatcaaattt cacccccgcc ggttgaataa cttcagggca
     3421 gaagtgtgtg gtaaaattca cgcacttctg ccttcctcct tgaaaaattt tcgagtgcag
     3481 agtacatttg cgtcgagaat ccgcttccca actgccgttg actccccaca ccatccaaaa
     3541 taaaaaggtt atgaaaccag ttgttgcgca gtttgtgtgt agcgccgttt ctccgcacca
     3601 ataccctaca gacagacttc ctgaaattgc atttcttgga cgctcaaacg ttggtaagtc
     3661 aagcctgatt aattccttgc ttcgggtcaa aggactggct cgcaccagca gcaccccagg
     3721 ccgaacacag ctcatcaact tctttcgcat taacaacgca ttgtattttg tcgatctgcc
     3781 tgggtacgga tatgcccgtg ttcctaccga aatccgacaa caatgggcac caatgattga
     3841 aaaatatctt attgaccggg ctccgctggc gctgggaatt gtgattgttg acagtcggtt
     3901 gaaaccaatg gatctggatc agcaaatggt tgaatggtta gacgcccaac acgttccact
     3961 gtgtgtggtg gcaaccaaag ctgataagct ctcaaacaac cagatggcca ctcagttaaa
     4021 gatactccga gagacatttg gccaggcggt catcccgtac tcagctttaa cccgcaaggg
     4081 cgccgacgaa gtctggcgga tgatccgaca ggcgggcgaa ccaaaaccac ccgcggtcat
     4141 caccgccacc agttaaagta ccagatcgtt cgcaccggat acccagacca ctcaccaggc
     4201 accccatgtg ctgctcgcag aattcgattt gtgatctccc aacaccattg tttatgcgtc
     4261 cgcaaaacgc agaccatctc acgttaacgt caaaggcacc agtaacctcg cctaccttgt
     4321 ttgaacttgt gacctgcctg aataccaagt tccacgtcgc acgatttcag tcttgaaacc
     4381 tgcaatctga aaccatttac caaattaatt gttcaagggc ttgaattgta tatggatgaa
     4441 cccattgaca catctaccaa cgggctaatg
//
LOCUS       DAOQDO010000187         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13162_length_4464_cov_3.272171, whole genome shotgun sequence.
ACCESSION   DAOQDO010000187 DAOQDO010000000
VERSION     DAOQDO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13162_length_4464_cov_3.272171"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKZ53_04195"
     CDS             <1..952
                     /locus_tag="PKZ53_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ39668.1"
                     /translation="RNPEPGTRNPEPGTRNPKQNEFPPMKDLSPCIDGPLPSGAETLG
                     PVLILDKVCKEFPLNDGNKFVALRDLSLTIENIRYKPQIVSLLGPSGVGKTTTLRLIA
                     GLDQPTRGKILITNGDSTPLRPVRVGDVGVVFQKYPLFEDISVLANLVEPAVRVGKMT
                     RKDAQQKALHFLDDFGLIKQGSAWPVHLSGGQRQRVAILQQLMIDRYFIILDEPFSGL
                     DPISIASVLRLITQVASQHELNTFILITHDVTSALSISDHVYLIGRERDAEGDPLPGA
                     RVIKTYDLIAEKIAYEPEVEDLPRFAEIRREIKLVEFPKL"
     gene            1008..1901
                     /locus_tag="PKZ53_04200"
     CDS             1008..1901
                     /locus_tag="PKZ53_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39669.1"
                     /translation="MPDKDINYVAEVLQSQPNYGFIGIMIFLMLVVDFWGFGALLAAG
                     EISCLFIAQNSRVQRIIRARKNKDKVLEIEEAEVTIVKVLPHPYQIDFQSVRNLCEEI
                     ERKSNELGRSNAQVMLANLIEKLSSFRYDYARMLRAHHLLSTRNYRNLQTSLTGEISR
                     VEQAIQREESTQVRHALNQNLNILKQRLGRIKKLEELVRLLEARLQVIKNSLSLIQDE
                     VYSFTDVTGISGLVDTLLTNLSLSDEFRSAYEEVLSAESNFSRLSDFEEPPAANVLAP
                     QPGDLPPTPSRNVRDQLRRVK"
     gene            2027..2740
                     /locus_tag="PKZ53_04205"
     CDS             2027..2740
                     /locus_tag="PKZ53_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39670.1"
                     /translation="MSKGFGKSGEADHPRPPKESGALKSLLNFFVKIVPGDEDTQTTS
                     GTDLAQSTADVVSDESLPVFAVPDETTGDLSKKPFAEIYREAGITESPLSVDELEKLL
                     TNPTIAAQPMSVKLIAINLALSAKGVTVDAPIADAARRDQVLESYQAMLLDRAREVER
                     RNSEKIQQITAEIEAYLKRYQAEIDTLHLETSELKRQSKDFALRRDAEAQRLANLINP
                     FLEGKPNPVLIHSQLETND"
     gene            2733..3098
                     /locus_tag="PKZ53_04210"
     CDS             2733..3098
                     /locus_tag="PKZ53_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39671.1"
                     /translation="MTEPTVTLYRPTGPQELELVRQSGWKRWPARLPEQPIFYPVTNE
                     AYACEIAQKWNVKESGVGFVTRFEVKASFMERYQPQQVGAAHHTEWWIPAEDLGALND
                     NLVGEIEVIREFRAEIVTE"
     gene            complement(3190..3708)
                     /locus_tag="PKZ53_04215"
     CDS             complement(3190..3708)
                     /locus_tag="PKZ53_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39672.1"
                     /translation="MFRLKTAATALGLVLAICLSAAAAQKTDLNGKWVMDKAKSEGLP
                     PDMEQTMNITQKGDELTIATTIKTSQGEQTLTDTYKLNGKPVEFTPSRPTPDGGTITG
                     KGTKTGTWTAEGKELELVQEEHLEVPSGEEVTIKITRNWHIAADGKSITIDLTAETPN
                     GPQSTKRLFVKQ"
     gene            4068..>4464
                     /locus_tag="PKZ53_04220"
     CDS             4068..>4464
                     /locus_tag="PKZ53_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ39673.1"
                     /translation="MSNFSARWNLTGHKALVTGGSKGIGFAIVEEFLGFGATVLSVAR
                     NEAVLEESVRGFREKNLPIHTVAADVGTPAGRAAIWAAVDEKLNGLDILVSNVGTNIR
                     KKVLDYTTEEILSLFNTNLFSALELTREAH"
BASE COUNT         1133 a         1066 c         1157 g         1108 t
ORIGIN      
        1 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc ctaagcagaa
       61 tgagtttcca cctatgaaag atctatcgcc ttgtattgac gggccattac ccagcggtgc
      121 cgagaccctg ggcccggttt tgatcctgga taaagtctgc aaagaatttc ctctgaatga
      181 tggcaataaa tttgtggcgc tccgggattt gtcgctgacg attgaaaata ttcgatacaa
      241 accccagatt gtcagccttc ttgggccatc tggggttgga aaaaccacga cgttgcgcct
      301 gattgccggg cttgaccagc cgacacgtgg gaaaatcctg attaccaacg gagactcaac
      361 gccgctacgt ccggttcggg taggggatgt cggggtggta tttcagaaat atccgttgtt
      421 tgaagatatt tcagtcctgg ccaatctggt tgagccggcg gtccgcgttg gaaaaatgac
      481 ccgcaaagac gcccagcaaa aagcgcttca ttttttggat gactttggat tgatcaagca
      541 aggatcggcc tggccagtcc acctttcggg tgggcagcgt cagcgggtgg cgattttgca
      601 gcagcttatg attgaccggt atttcatcat tctggatgaa cctttttcag ggcttgatcc
      661 gatcagcatt gccagtgttt tgcggctgat tacccaggtt gccagtcaac atgaactcaa
      721 cacgtttatt ctcattaccc atgatgtgac ctcggcgctc tccatctcag accatgtgta
      781 tttgattggc cgggagcgtg atgccgaggg tgatccactc ccgggcgccc gagtcatcaa
      841 gacctatgac ctgattgcgg aaaaaattgc ctatgaacct gaggttgaag accttccgcg
      901 atttgctgaa attcggcgcg aaataaaatt ggtcgaattc ccaaaattat gacccgccgg
      961 tccggctttt tctgattttg ttttgaagca aagatgagtg aaggtgtatg cctgacaaag
     1021 acatcaatta tgtggctgaa gttctgcaaa gccaaccaaa ttatggtttt atcggcatca
     1081 tgatttttct gatgctcgtg gtggattttt ggggatttgg tgccttgctc gcggctgggg
     1141 aaatcagttg tttgtttatt gcccaaaatt ccagagtcca gcgcatcatc cgggcacgca
     1201 aaaacaagga caaggttctt gagatcgaag aggctgaagt cacgattgtc aaagtgttgc
     1261 ctcatccata tcaaatagat tttcagtcgg ttcggaactt gtgcgaagaa atcgaacgga
     1321 aatccaatga gctgggccgg tcaaacgctc aggtgatgct ggccaacctg atcgaaaagc
     1381 tttcgtcatt tcgctatgac tacgcccgga tgttgcgagc ccaccatctg ctctcaaccc
     1441 gcaattaccg aaatttgcaa acgtcactga cgggtgaaat ttcccgggtc gagcaagcca
     1501 tccaacgcga agaatcaacc caggttcgcc acgcgctcaa tcaaaacctc aacattttga
     1561 aacagcgact ggggcgaatc aaaaaactcg aagaacttgt tcgattgctg gaggcccggc
     1621 tgcaggtcat caaaaactca ctcagcctga ttcaggacga agtgtactca tttaccgacg
     1681 tgacgggcat ttctggtctg gttgatacgt tgctcaccaa tttaagcctg agtgatgaat
     1741 ttcggtcagc ctatgaagaa gttctcagcg ccgaatcaaa tttttcaagg ctatcggatt
     1801 ttgaagagcc accagcggcg aatgtgctag cccctcaacc tggtgacctg ccgccaaccc
     1861 cgtcgcgcaa tgtgcgggat caactccgcc gggtgaagtg accggaattc ctttcgcccg
     1921 gtatttgaac tcttcacgct caactgtgaa agcgaggaat atgacaaact ctcaaactac
     1981 acctaaaccc agaaccaccg aagagctttt gcaggaaatg gaaacgatgt cgaagggctt
     2041 cggaaaatct ggcgaagcgg accatccccg accaccaaaa gaatctggcg ccctcaaatc
     2101 gctcctcaac ttctttgtca aaatcgtgcc tggagacgaa gacacccaga caacgagtgg
     2161 cactgattta gcacagagta cggcagatgt tgtgagtgat gaatcacttc cagtctttgc
     2221 cgtgccagac gagacaaccg gggacttgtc caaaaaacca tttgctgaga tttatcggga
     2281 agctggtatc accgaaagcc cgttgagtgt tgatgaattg gaaaaattgc tcaccaaccc
     2341 aaccattgcg gctcaaccga tgagcgtcaa actgatcgcc atcaatctgg cgctgtcagc
     2401 caaaggcgtc acggttgatg cgcccattgc tgatgccgcc cggcgggatc aggtactgga
     2461 atcatatcaa gccatgctgc ttgaccgggc acgtgaagtt gagcggcgga attcagaaaa
     2521 aattcagcag atcactgccg aaatcgaagc gtacttgaaa cgctaccagg ctgagattga
     2581 tactctgcat ctggaaacca gcgaactcaa acgccagtca aaagattttg cccttcgacg
     2641 cgacgctgaa gcccaacggc tggccaacct catcaaccct tttctggaag gaaagcccaa
     2701 tccggtgctg attcattccc agcttgaaac aaatgactga accaaccgtg acactctatc
     2761 gcccgaccgg gccacaagaa cttgaacttg ttcgccaaag cggatggaaa cggtggccgg
     2821 cgcgactccc ggaacaaccg atcttttatc cggtgaccaa cgaagcctat gcctgcgaaa
     2881 ttgcccaaaa gtggaatgta aaagaaagtg gtgtgggttt tgtaacccgg tttgaggtca
     2941 aagccagctt tatggagcgg taccagccgc aacaggtggg cgccgcccat cacactgaat
     3001 ggtggattcc agccgaggat cttggggcac tgaatgacaa tctggtggga gaaattgagg
     3061 taattcggga gtttcgagca gaaatagtga cggagtgaag tagcgaaata gttgaaggca
     3121 acgagtttag gcggtgacag atccgtttgg aaaatcaaca ctgagctatt tcgctacttc
     3181 actttttgct tattgtttga caaatagccg cttggtggat tgtgggccat tgggcgtttc
     3241 agccgtcaga tcaatggtga ttgatttgcc atcagccgca atgtgccagt tacgcgtgat
     3301 tttgatggtc acttcctctc cactgggaac ttcaagatgt tcttcttgaa caagttctaa
     3361 ttctttccct tcagccgtcc aggtccctgt tttggtgcct ttacccgtaa tggttcctcc
     3421 atctggagtt gggcgagagg gggtaaactc cactggcttg ccattgagtt tgtaggtatc
     3481 ggttaaagtt tgttcgccct ggctcgtttt aatggtggtt gcgatggtta attcgtcgcc
     3541 tttttgagtg atgttcatgg tctgttccat atcagggggc agaccttcgc ttttcgcttt
     3601 gtccatgacc cattttccat tcagatcagt tttttgagcc gcggcggcgc tcaaacaaat
     3661 tgccagtacc agacccaatg cagttgctgc agttttcaga cgaaacatgt gaattctcct
     3721 tagaatattg aaccggaatg atctttgaat tgaagatcaa ataattggtg gcgacggttg
     3781 gaagcgtatt ttgtgaaaca tggttgtgaa gcggcttcag aaacgaagct gaatcagaaa
     3841 tgttcaagcc gttgaatgaa atcctggttg tgatcatagg ggttggggca aaaggtgtcc
     3901 ataggatcca ggtttggagc tgagaaggtc aggttgaaga ttttcagact cagggatgaa
     3961 gacatcaaaa tgaaaaacgg aggccgttct tttggattgc tggaatcgtc tcgtcaaaca
     4021 acaatgcagc gattgatcaa tctttttcca ataagggagt tggcactatg tcgaattttt
     4081 cagcccggtg gaatctgacc gggcacaaag cactggtcac tggcggttca aaaggcatcg
     4141 ggtttgccat tgtcgaagaa ttcctggggt ttggggccac ggttctttcc gtcgctcgga
     4201 acgaagccgt tttagaagag agcgtccgtg gttttcgtga aaaaaatctt ccgatccata
     4261 ccgtggcggc tgatgtcgga acacctgcgg ggcgagcggc gatttgggcg gcggttgacg
     4321 aaaaattgaa cggattggat attttggtga gcaatgtcgg cacgaacatc cgcaaaaaag
     4381 tgcttgatta caccacggaa gaaatcctga gtctctttaa caccaacctt ttctcggctc
     4441 tggagttgac ccgcgaagcc catc
//
LOCUS       DAOQDO010000188         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13184_length_4461_cov_2.400590, whole genome shotgun sequence.
ACCESSION   DAOQDO010000188 DAOQDO010000000
VERSION     DAOQDO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13184_length_4461_cov_2.400590"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKZ53_04225"
     CDS             complement(<1..336)
                     /locus_tag="PKZ53_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ39674.1"
                     /translation="MHHNVIFFAHGKESGPWGSKIQRLAEVGKAKGFAVESPDYAGID
                     DPDERVRRLLALNPKAIYRLVLVGSSMGAYVSLVASRTICPDGLFLLAPAVHIPFYPE
                     FEPPPQARQV"
     gene            473..1747
                     /locus_tag="PKZ53_04230"
     CDS             473..1747
                     /locus_tag="PKZ53_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ39675.1"
                     /translation="MTTLAIIGTGIAGLGCAHFLHQRFDLTLFEQNDYVGGHTNTVEA
                     DEGGKTVPIDTGFMVYNEVTYPNLTRLFRELQVRVKPTTMSFSVQHIPTGLEFCGSSI
                     NHLFAQRRNLLRPRFLIMLAKIDRFNKEAVDALNEHRFQNYTVQQYIQERQYGDDLLN
                     LYLIPMSSAVWSTPPDKMLEFPAVTLLRFFYNHGFLGLYTQHPWLTVDQGAKSYVAKI
                     TAPFHQKIRVNQPVVRVTRTSNQVAIQTRDGATQTFDKVIFACHGDQALRLLGDATPD
                     EHRLLSAFSYQPNLAILHTDASLMPQRRLAWSSWNYRIELDERGKVSPTTIYWMNSLQ
                     GVSDKQNYFVSINGEHRINPQRILKRIAYEHPLFSLKAINAQFELPKLNQISPLQTTY
                     FCGSYFRYGFHEDALTSALELSRTITGKPIWK"
     gene            1784..2638
                     /locus_tag="PKZ53_04235"
     CDS             1784..2638
                     /locus_tag="PKZ53_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNJ39676.1"
                     /translation="MSTVEATSLISTGFSNSCLYECRVMHARLQPKVHRFEYRVFMMY
                     LDLDEIPALARRLWSFSHERFNVYAFRASDHFIGSAPSLKENIIHFAGQHGVDVAGGR
                     VTLLTLPRIAGYIFNPVSFYFCFDRQGVPACAIAEVNNTFHETKPYFVPKIDAHSDHP
                     SRFRLLAPKYFYVSPFSELDFQFEFKLQLPDESLKIYVNDFNGENKMLVSSLLGTRKP
                     LTDARLMWFVLKYPLLTLQVIGGIHYQALLLYLKKIPFHRKAANPTLQRDVIHPHHSI
                     AGRTHEIP"
     gene            2635..3942
                     /locus_tag="PKZ53_04240"
     CDS             2635..3942
                     /locus_tag="PKZ53_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNJ39677.1"
                     /translation="MSSQPDQTNFLESPTSSSPESSTSVTSAAPESFGLYQRIVIGVL
                     EQMTRGCLNLEFPDGSTRTIGQPGAANSARIRILNPVFFKKCVLYGDIGFGEAYVDGD
                     WDTDDITRVIAWAINNIDTIQVMSGSKGKSWLVNIFGFLNRVYHLMRPNSLRTSRRNI
                     QEHYDLGNAFYQTFLDSTMTYSCAYFAASDQSLEAAQLAKYDALCRKLKLKATDHVLE
                     IGTGWGGFSRYAAKTFGCRITTVTISEEQFKYAKALFDREGLADQIEIRLQDYRHIQG
                     EFDKVVSIEMIEAVGDAFLETYFQKCQDVLKKDGLLALQMITCPDSRYELLRKGVDWI
                     QKHIFPGSLLVSVGRVNQAINRTSDLYLHDLEDMGLFYARTLRLWRETFCQKLDQARA
                     LGFNDVFIRKWLYYLSYCEAAFAMRNISVVQVVYTRPNNPTLF"
     gene            3939..4319
                     /locus_tag="PKZ53_04245"
     CDS             3939..4319
                     /locus_tag="PKZ53_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNJ39678.1"
                     /translation="MTHKAASSRRTPKNIMIWTLRIFFLFVLASMLAVTSWASMDTAL
                     WKIPREVVAHPWFIATLFDTYFGFLTFYLWVIYKESSWLARIAWLIAILLLGNIAMAV
                     YALIQLFRVSSDARIEDVLVKAQS"
BASE COUNT         1088 a         1099 c         1074 g         1200 t
ORIGIN      
        1 aacctgacgg gcctgaggcg gcggttcaaa ttctggataa aagggaatgt gaaccgccgg
       61 tgccagtaaa aaaaggccgt ccgggcaaat ggttcttgaa gccaccagcg aaacataggc
      121 tcccatgctt gaaccgacca aaaccagccg ataaatggct tttgggttga gggcaagcaa
      181 gcgtctgacc cgttcatcag gatcatcaat cccggcataa tcggggcttt ctacggcaaa
      241 tcctttggct ttgccaacct cagccagccg ttggattttg cttccccagg ggccgctttc
      301 tttcccatga gcaaaaaaaa tgacgttatg gtgcataatt tggtaatctc ccacggagtt
      361 gacttctttc tactcgaaat tcctgttaaa aaattagtcc aagctttttc cattccccag
      421 taccaaccag caacatcgag aatttttacc cagactccag gaaagggaag ccgtgacgac
      481 tcttgccatc attggaaccg ggattgccgg ccttgggtgc gctcattttc tgcaccagcg
      541 atttgatttg acgctttttg aacaaaacga ctatgtcggt ggccacacaa atacggttga
      601 agctgacgaa ggtgggaaaa ctgttcccat tgacaccggg tttatggtgt acaacgaagt
      661 cacctatccg aatttgaccc gccttttccg cgaattgcaa gtcagggtca aaccaaccac
      721 catgtcgttt agtgtccagc acatccccac tggcctggag ttttgtggct ccagtatcaa
      781 tcatttattt gcacagcgac gaaatttatt acgaccccgc tttctgatca tgctcgccaa
      841 gattgaccgc ttcaacaaag aggcggttga tgccctgaac gagcaccggt ttcaaaacta
      901 taccgttcaa caatatatcc aggagcgcca gtatggcgat gatttgctca acctctattt
      961 gattccgatg agttcggcgg tctggtcaac gccaccggat aaaatgctgg aattcccggc
     1021 ggtgaccttg ctgcggtttt tctacaatca cggctttttg gggctctaca cccagcatcc
     1081 ctggttaacg gttgatcagg gggcgaaatc gtatgttgcc aaaatcacag ccccctttca
     1141 ccagaaaatt agagtcaatc agcctgtggt tcgggtgacc cgcacgtcaa atcaggttgc
     1201 catccagacg cgggatggag cgactcaaac ctttgacaag gtgatttttg cctgtcacgg
     1261 cgaccaggcc ctccggttat tgggcgacgc aacgccggat gaacaccgct tgctcagtgc
     1321 gttttcctac cagccgaatc tggccatttt acatacggat gccagcttaa tgccccagag
     1381 aagactggcc tggtcgtcgt ggaattaccg gattgagctc gatgaacgag gaaaagtgtc
     1441 tccgacaact atatattgga tgaatagttt acagggagtc tcggataaac agaattattt
     1501 tgtttcaatc aatggtgagc atcgaatcaa ccctcagcgc attctcaaac ggattgcata
     1561 tgaacaccca ctcttttccc tcaaggccat caatgcgcaa tttgaacttc caaagctgaa
     1621 ccagatatca cctcttcaga ccacatattt ttgtggcagt tatttcagat atgggtttca
     1681 cgaagatgcg ttgacctcag cccttgagct ttcccgaacc attaccggta agccaatctg
     1741 gaagtgatcg ttgagaaatg agatacaacc tgacgaaatg acaatgagca ctgttgaagc
     1801 cacctctctc atatcaactg gattctcaaa ttcgtgcctg tacgaatgcc gggtgatgca
     1861 cgcccgcctg cagccaaaag tgcaccggtt tgaatatcgg gtatttatga tgtacctgga
     1921 tttagatgaa ataccggcgc ttgcccggcg cttgtggagt tttagccacg agcgattcaa
     1981 tgtgtatgcg tttcgagcgt ctgaccattt catcggatca gcgccgagct tgaaggaaaa
     2041 catcattcac tttgccgggc agcacggggt ggacgttgct ggtggccgag tgacattgct
     2101 gacgttgcca cgaattgccg gatatatctt taatccggtt tctttttact tctgttttga
     2161 tcgccagggg gttcctgcct gtgccattgc ggaagtaaat aacacgtttc atgaaaccaa
     2221 accttatttt gttccgaaga tagatgcaca cagtgatcat ccatcacgct ttcgcttact
     2281 ggcccccaag tatttctacg tttcgccctt ttctgagctg gattttcaat ttgagttcaa
     2341 actgcaattg ccggacgaga gtctgaaaat ctatgtcaac gattttaacg gtgaaaacaa
     2401 aatgctggtc agttcgctgc ttgggacgcg caaaccgctc accgatgccc gattgatgtg
     2461 gtttgttttg aaatatccgt tgctcacctt gcaagtgatt ggtggcattc actatcaggc
     2521 acttttactg tatttgaaaa agataccttt ccatcgcaaa gcagcgaacc ccacgttgca
     2581 gcgcgatgtc attcaccccc atcattctat tgctggaagg acacacgaga tcccatgagt
     2641 tcgcagccgg accagaccaa ttttttggaa tccccaactt cttcatcgcc agaatcttcg
     2701 acatcagtga cttcagccgc acctgagtca tttggattgt atcagcgcat tgtgatcggg
     2761 gtgcttgaac agatgacccg ggggtgtttg aacctcgaat ttcctgacgg tagcactcga
     2821 acgattggcc agcctggggc ggccaacagc gctcgcattc ggattctcaa ccccgtgttc
     2881 ttcaaaaagt gcgtgttgta tggtgatatc gggtttggcg aagcctatgt tgacggagat
     2941 tgggatactg acgatatcac gcgcgtgatc gcgtgggcga tcaacaatat tgacaccatt
     3001 caagtgatgt caggatcaaa agggaagtcc tggctggtca atattttcgg ttttctgaac
     3061 cgggtctatc acctgatgcg gcccaacagt cttcggacca gccggcgcaa catccaggag
     3121 cactatgact tggggaatgc gttttaccag acatttcttg attcgacgat gacatattcc
     3181 tgtgcctatt ttgcagccag cgatcaatcg cttgaagccg ctcaactggc gaaatatgac
     3241 gccctgtgcc gtaaactcaa gctcaaagcc accgatcatg tgcttgaaat tggaactgga
     3301 tggggtgggt ttagccgcta tgctgccaaa acctttggct gtcggattac cacagtgacc
     3361 atttctgaag agcaatttaa atacgccaaa gccttgtttg accgtgaggg actcgctgac
     3421 cagattgaga tccgactcca ggattaccgt catatccagg gagaatttga caaggttgtt
     3481 tcaattgaaa tgattgaagc ggttggagat gcattcctcg aaacctattt tcaaaagtgt
     3541 caggacgttc tcaaaaaaga tggattactg gccttgcaga tgattacctg tccagattcc
     3601 cggtatgaat tgctgcgcaa aggggttgat tggattcaaa aacacatttt tcctgggtcg
     3661 ttgctggttt cagtcggaag ggtcaaccag gcgatcaatc gaaccagcga cctgtatctg
     3721 catgacctgg aggatatggg gttattttat gcccgaaccc tccggctctg gcgcgaaacc
     3781 ttttgtcaaa aactggacca ggctcgtgcc ctcgggttta acgacgtatt tatccgtaag
     3841 tggttgtatt acttgagtta ctgtgaagct gcctttgcta tgcgcaacat cagcgtggtg
     3901 caggtggtct atacccgacc caataatcca acgctctttt gacccacaaa gcggcgtcaa
     3961 gccgccgcac tccaaagaac attatgatct ggacgttgcg cattttcttt ctttttgtgt
     4021 tggccagcat gctggcggtg accagttggg ccagtatgga tacggcgcta tggaagattc
     4081 ctcgcgaggt ggtcgcccat ccctggttta tcgcaacact gtttgatacc tactttggat
     4141 tcttaacttt ttatctatgg gtaatttaca aggaatcatc ctggttagcc cgtattgcgt
     4201 ggctgattgc tattttgctt ctgggaaata ttgccatggc tgtttacgcc ctgattcagt
     4261 tgtttcgcgt gtcatctgat gcccggattg aagatgttct ggtcaaggcg caatcctgac
     4321 aaagtgactg aatgacaagg tgacaatttt ccgaaaaccc ggaacccgga accctaaaac
     4381 atatgctttc tgaaatcctg ctcttaactg gaattgcgct ggccgttgct tcggtgatga
     4441 tggtcatcat ctggctgatt g
//
LOCUS       DAOQDO010000189         4458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13197_length_4458_cov_2.979105, whole genome shotgun sequence.
ACCESSION   DAOQDO010000189 DAOQDO010000000
VERSION     DAOQDO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13197_length_4458_cov_2.979105"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="glk"
                     /locus_tag="PKZ53_04250"
     CDS             <1..945
                     /gene="glk"
                     /locus_tag="PKZ53_04250"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNJ39679.1"
                     /translation="TKTNVALFRESDLSLIHNRSFPSQNYSGLWPILEEFLQEAKGDI
                     SNACFGVAGPVNNGISSITNLSWTIEAADLRENLKCTSVHLINDLEANGWGIEVLPSS
                     SFHVLREGTPKPGNAALISAGTGLGECCLFWNGTRHIPSASEGGHASFAPRNEDEVEL
                     LRYLWKQYSHVSWERVVSGSMGFQNIYNFYRDTHRAIEPSDLAAKIQTGDPAAIISQS
                     ASQDIDIAVKTMEMFFTLYGAEAGNLALKIIAVAGVYIGGGIAPKNLNWMTSGSFLDS
                     FSAKGRFTSMLEEIPVKIILDSQTALYGAARFAQLKGE"
     gene            1198..2742
                     /locus_tag="PKZ53_04255"
     CDS             1198..2742
                     /locus_tag="PKZ53_04255"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNJ39680.1"
                     /translation="MKNTHSVLAIDIGTSSVRAVLFDTQGHPVPGAEARSTYQVHYTS
                     DGGCEIPADELFSWVLQTISSCQAQVAAADVIGVGISCFWHSLVGITPDTCAPATPVY
                     LWADTRSRGVLDEIKELEAEAELFARTGCRVHTTYWPAKIRWIKASQPSLHFSALWWM
                     SFAEYLGLRLFGERVVSVSMASGTGVFHQELGHWDPPTLDLCGLTQSQLSTIAPDTLC
                     FSGERLRALYKEQLGRLTSAQWFLSLGDGACSNLGSGPPCQTAAVLNLGTSAALRLSI
                     PETFQKLKTPALARQLFTGGLWQYRIDQQWHLVGGALSNAGNLLGWLREVLNLPSTAE
                     INQFLSDSIPDSHGLSCLPFLAGERSPGWSADTFGLVAGLHFQTTPLQLVQATIETIA
                     LRLAWILENLHLAGFPIDHIIASGGALTAFPAWAQIICDALNRPVLRLENAEVSARGA
                     ALWVFHQLGLQDLAQSQIPASTLFTPDPARHHIYRQALAHHQQFYHTFRHQPVRPARP
                     NRLPSS"
     gene            3006..>4458
                     /gene="leuS"
                     /locus_tag="PKZ53_04260"
     CDS             3006..>4458
                     /gene="leuS"
                     /locus_tag="PKZ53_04260"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNJ39681.1"
                     /translation="MNEKYLPQDVERKWQKRWAEDHTFEVHPDPNQPKFYSLEMLPYP
                     SGRIHIGHVRNYSLGDAFAWYKRLNGFNVLHPIGWDAFGMPAENAAIKHGQPPDTWTR
                     SNIAVMRDQLKRVGFSYDWSREIASCTPEYYHWNQWFFIQMFKKGLVYRKLATVNWCD
                     KCQTSLANEQAEGGFCWRHEDTPVEKRELKQWYVRLTHYNEELLAGINGQLQGNWPER
                     VLTMQRNWIGRSEGALVDFALAHDSSRSIKVFTTRIDTIFGASAVVIAPEHPLVRHLL
                     AQSPNREPVEAFINQVSKMSTMDRIGEGTTKEGISTGLCAVNPFNQEQLPIWVANFVL
                     AEYGTGAIMCVPAHDERDYEFCRKYHLPIQRVIWGYTESDANVTLRFSELPFTDPGFL
                     GPNCGQYAGLPSDEAQVTMAQFAETNGFGKATVTYRIRDWGISRQRAWGTPIPMIHCP
                     ACGIVPAREEDLPIKLPDNLDFSVGAPLTRCAEY"
BASE COUNT         1063 a         1246 c         1108 g         1041 t
ORIGIN      
        1 accaaaacca atgtcgcgct ttttcgcgaa tcagacttaa gcctgattca taaccggagc
       61 tttccaagtc aaaactactc gggactttgg ccaattcttg aagaatttct tcaagaagcc
      121 aaaggagaca tttccaacgc ttgttttggc gtggcaggac ctgtcaacaa cggaatcagc
      181 tcgattacaa acctgagctg gaccattgaa gcagcagatt taagggaaaa tctgaagtgt
      241 acgagtgttc atctgatcaa cgatttagag gcgaatggtt gggggattga agtccttcct
      301 tcatcttcat ttcatgtatt acgtgaaggc acccccaaac cgggaaatgc cgcgttgatc
      361 tcggcaggga ccggactggg agaatgttgt ctcttttgga acggcacccg gcatattccg
      421 tcagcctcag aaggggggca tgccagtttt gcgccccgga atgaagacga agtagaactc
      481 ctcagatatt tatggaaaca atattcccac gtcagttggg aacgggtggt ttctggaagt
      541 atgggcttcc aaaatatcta caatttttat cgcgatacac accgagccat tgaaccttcc
      601 gacctggccg cgaaaattca aactggcgac ccggcggcca ttatcagcca gtcagccagc
      661 caggacattg atattgcggt gaagaccatg gaaatgttct ttacactcta tggtgcggaa
      721 gcgggcaatc tggcattgaa aattattgca gtggcagggg tttatatcgg tggaggaata
      781 gcccctaaaa atctgaactg gatgacctca ggaagctttc tggactcttt ttcagccaaa
      841 ggccgattta cctcaatgct tgaagagatt ccagtcaaaa ttatcctgga tagccagacc
      901 gcactgtatg gtgccgcaag atttgcccaa ctcaaaggcg agtagcacgg cggaattcca
      961 ggaatggaat tatgaagtga tcagttattt ccaacactct caaatatctg ctcatacttc
     1021 ataatccata attcataatc cataatctct caactgcctc tggattgaat gataatcaat
     1081 tcagaggcag tttgctttct cctgacctga ctgtaaatta cagtaagaaa ccagagcagt
     1141 aattacataa tttaccacaa ggattaccat agtaatatca ttacactatt actcattatg
     1201 aaaaataccc actctgttct ggcaattgat attgggacat cctcagtgcg cgccgtcctg
     1261 tttgacaccc agggacatcc ggtacctggc gctgaagccc ggagcaccta ccaggtccat
     1321 tacacttcag atggcggctg tgaaatacca gccgacgaac ttttttcctg ggtacttcag
     1381 accatctcaa gctgccaggc tcaagttgca gccgctgatg tcatcggggt ggggatttcc
     1441 tgtttctggc actcgctggt tggaattaca cccgatacct gtgcaccagc caccccggtg
     1501 tacctgtggg cagataccag gtcacgcggg gtgcttgatg agataaaaga acttgaagcg
     1561 gaagctgagc tttttgcccg aacgggctgc cgggttcata ccacctactg gccagccaaa
     1621 atacgctgga tcaaagccag ccagccatca ctccacttct ctgccctgtg gtggatgtca
     1681 tttgccgaat atctgggact ccggttgttt ggggaacggg tggtgagtgt ctcaatggct
     1741 tcagggacgg gagtatttca tcaagaactg ggccactggg atccaccaac cctcgatcta
     1801 tgtggcctga cacagtcaca actttcaacg attgccccag acaccctgtg tttttcagga
     1861 gaaaggctgc gtgcgctata caaggagcag ctaggtagac tcacgtcagc acaatggttt
     1921 ctttcgcttg gggacggcgc ctgcagtaat ctcgggagcg gcccaccctg ccagacggca
     1981 gccgtcctca accttggcac ctcggcagcc ctgcgactga gcatccccga aacgttccaa
     2041 aaactcaaaa ctccggctct ggcccggcaa ctgttcaccg gggggctctg gcagtatcgc
     2101 attgaccagc agtggcattt agttggtggt gcgctgagca atgctggaaa tctgctgggc
     2161 tggctgcgtg aggtcctcaa ccttccatca accgctgaga tcaaccagtt tttatcagac
     2221 tctatacctg actctcatgg cttgagttgt ctgcccttcc tcgccggaga acgcagccca
     2281 ggctggagtg ctgatacctt cggtctggtg gcgggccttc acttccagac cactcccctt
     2341 caactggtcc aggcaaccat tgaaaccatt gcgctccgtc tggcctggat cttagaaaat
     2401 ctgcacctgg ctgggtttcc aattgatcac attattgcct cgggcggggc gctgacggca
     2461 tttccggcct gggcccaaat catctgtgac gccctgaacc ggccagttct tcggctcgaa
     2521 aacgccgagg tttcagcccg gggtgccgca ctgtgggtct tccatcagtt gggtctccag
     2581 gatctggctc aatcacagat cccggccagt acgcttttta ctccggatcc agcccggcac
     2641 cacatctatc gtcaggccct ggcccaccat caacagttct atcacacctt ccgtcaccag
     2701 cctgtgcgcc cggcacgacc caaccggttg cccagctcct gaggacggac cggcaatgac
     2761 cagctctcga ccagagcacc gaaaagcccc attgtgcgga ctggttttct cgaaaaaccc
     2821 caaaccccaa acccttcccc ttgacacctg ccacgccaac tctgatgatg ctctctttca
     2881 ctgcttttgg tgaatctttg gtggcaacgc tttgcattga gcatcatttt ggtccataca
     2941 ctttcccgat gactccccct ggattttggg aacctgtgga cacgaaagaa ggacatcgaa
     3001 caacaatgaa cgagaagtac ctcccgcaag acgtcgaacg caaatggcaa aaacggtggg
     3061 ctgaggacca tacctttgag gtccacccgg atccgaatca acccaaattc tattcactgg
     3121 aaatgcttcc ctacccctcc gggcgcatcc acattggtca cgtccgtaac tattcgctgg
     3181 gagacgcatt tgcctggtat aagcgcctga acgggtttaa cgtgcttcac ccgattggat
     3241 gggatgcctt tggaatgccg gctgaaaatg ccgccatcaa acacggccag ccgcctgaca
     3301 cctggacgcg gtcaaatatt gccgtcatgc gcgaccagct caaacgcgtt gggttcagct
     3361 atgactggtc ccgtgaaatc gcatcctgta cgcccgagta ttaccactgg aaccaatggt
     3421 tcttcattca aatgttcaaa aaagggctgg tgtatcgcaa gctcgccacc gtcaactggt
     3481 gcgacaagtg tcagaccagt ctggccaacg aacaggcgga aggcgggttt tgctggcgac
     3541 atgaagacac cccggttgaa aaacgtgagc ttaaacaatg gtacgtccgc ctcacccact
     3601 ataacgagga attgctggct ggaatcaatg gccaactgca gggcaactgg ccggaacggg
     3661 tactcaccat gcagcgcaac tggatcggtc ggagcgaagg cgccctggtt gattttgccc
     3721 tcgcccacga ttcatccagg tcaatcaaag tctttaccac ccggattgat actatctttg
     3781 gggccagcgc cgttgtgatc gcgccggaac acccacttgt caggcatctg ctcgcccagt
     3841 cacccaatcg tgaaccggtt gaagcattta tcaaccaggt cagcaaaatg agcaccatgg
     3901 atcgaattgg cgaaggcacc accaaagaag gcatcagcac cggactgtgt gccgtcaatc
     3961 cgtttaatca ggaacagctc ccgatctggg tggccaattt cgtcctggcc gaatacggca
     4021 ccggggcaat tatgtgtgtt cccgcccacg atgagcggga ctatgagttc tgccgcaaat
     4081 accatctgcc catccagcgt gtgatttggg ggtataccga gtcagatgcc aatgtcaccc
     4141 ttcggttttc ggaactgccc tttactgatc cagggtttct cggaccaaat tgcggccagt
     4201 atgcgggcct cccttcggat gaagcccagg ttacgatggc tcaatttgct gaaaccaatg
     4261 gatttgggaa ggcaactgtc acctaccgga tccgtgactg gggtatttcc cgccaacgcg
     4321 cctggggcac ccccatcccg atgattcatt gtccagcctg cggtattgtg ccggctcgtg
     4381 aagaagacct gcctatcaaa ttgcctgaca acctggattt ctcagtcggg gccccgctga
     4441 cccgctgtgc cgaatatg
//
LOCUS       DAOQDO010000190         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13205_length_4457_cov_2.823489, whole genome shotgun sequence.
ACCESSION   DAOQDO010000190 DAOQDO010000000
VERSION     DAOQDO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13205_length_4457_cov_2.823489"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..359)
                     /locus_tag="PKZ53_04265"
                     /pseudo
     CDS             complement(63..359)
                     /locus_tag="PKZ53_04265"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(375..2156)
                     /locus_tag="PKZ53_04270"
     CDS             complement(375..2156)
                     /locus_tag="PKZ53_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39682.1"
                     /translation="MKPGRYWIKLCLMCCLSIWLWGASVPLLAQSSATQTTVYTLETR
                     SDAAKTLSDQANQLYKTQDYKSALAKIQAALNQDPNSYKLHNLYVDIQLMLVSPDRLR
                     QEYLDKMAKDPTNPVFPLILARNQILLVNGEGRQELLEKAGAVAPDSVVGQSALIDLY
                     RFHKEDLAKAEEAYKKAIALDPNNHEMIANLAAFQVEELKKFDEALALYQSLLKQNPK
                     NYEAAIGVMSVNVAKAGYSDKAKAAMRQELTALKASEPPSPNLLSAIRLACWLVLKDQ
                     TAAQEITKELDDKFPTPGAPPGLMGISMFTDTGEPIQYIFAGRRYFWIRKIQTIEADQ
                     KTPLADRIAKLETLNQENPDDEQFRAYLLERLVRLYKEAKDTAKQEATVKQILAIEPR
                     QVGLLNELAWSLTAGARTDQEKALTYAKLAIAELEKLTQGKPGTGYNQQYVQQSKASF
                     LETLGRIQFQLDNLSEAETAFTQSLALNQTDDARYELGRVYKRQGKTREAAQIFAEVV
                     ASEGDNSAMARKWLEDLGQADTSIHASALIATATEKRISRLREKVIASLVTKPSKDFT
                     LTSFDGKKVNLASLKGKVVMLNFWASW"
     gene            complement(2402..3340)
                     /gene="pip"
                     /locus_tag="PKZ53_04275"
     CDS             complement(2402..3340)
                     /gene="pip"
                     /locus_tag="PKZ53_04275"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNJ39683.1"
                     /translation="MRTLYDPIEPYDQGMLKVSDIHTLYYEQSGNPNGRPVVFLHGGP
                     GSGTSPKHRQFFDPQAYRIILFDQRGSGKSLPFASLEENTTWDLVADTEKIREHLGID
                     TWVVFGGSWGSTLALAYAETHPERVQALILRGIFLCTKKELAWFYQDGAGAIYPDQWE
                     PFLNFIPEAERGDMMNAYYRRLTSDDEALRLEAAKIWSIWEGSTCTLYPDPDLVTEFG
                     ADDKALSIARIECHYFVNNIFMEEDQLLKDVDRIRHIPAVIVQGRYDIVCPMMTAWAL
                     HKAWPEAEFHVIPDAGHSAFEPGITDELIKASDRFR"
     gene            3476..4291
                     /locus_tag="PKZ53_04280"
     CDS             3476..4291
                     /locus_tag="PKZ53_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNJ39684.1"
                     /translation="MNHHQLEWSHRGFFVIRQLLTRLDQIFLKQVCDRIFNQWLAESP
                     DPTEAANSTNLAYLTEPRYFSHHPQELVWLLELIASETVFGPVQSVCDRELIFHNTQY
                     FFEPATHTRDGNWHRDQQFGATDEAEEQRWMATTIGVHIHIALEPDDHLEIVPGTNAR
                     WDTPAERSIRKGLNGIARNAPDMPGAVRIDLQPGDACVFNAWSIHRGRYVAGKPRRTF
                     DVIYGTKPDSYVTPPTCFLDPKHLDGISQSAHLFYQKKMRESPILSREAAGRG"
BASE COUNT         1084 a         1087 c         1124 g         1162 t
ORIGIN      
        1 acgaatcaaa ataatttggg taatgacaag acccggaacc cggaacccgg aaccccaaac
       61 cctcatttgg ctttgagcaa ttcctcgatc aggattgaga attcccgtgg accgtctttg
      121 ggattaaatc cggtttctgt tttgcggata tttccctgac gatcaataaa gaaggtaatc
      181 ggaatcccac gcacgccata gagttctttc acctgtttgg cttcaaacac ctgaaatcca
      241 ggtttttgtt gtttgacata tgttttggca gcattcaggt catcgtcaac gttgattgag
      301 aggagttcaa accctttggc tttgtaggta ttgtgcaagg tgataaggtg cggaaactcc
      361 gcacgacagg gccctcacca actcgcccaa aaattgagca tgaccacctt tcctttgagt
      421 gaggccagat tgaccttttt cccatcaaaa ctggtcaagg tgaaatcttt cgatggtttg
      481 gtaaccagtg aggcaatcac tttttcccgg agcctcgaaa tccgcttttc ggttgccgta
      541 gcgatcaggg cagaggcatg aattgaggtg tctgcctgac caagatcttc taaccatttc
      601 ctggccatgg cggaattatc gccttcactg gcaaccactt cggcaaaaat ctgggcggct
      661 tctctggttt tgccttgtct tttatagacc cgacccagtt cataccttgc atcatccgtt
      721 tgattgaggg cgagagattg ggtgaatgct gtttcagcct ctgaaaggtt atccaattga
      781 aactgaattc tccccagagt ttcaaggaac gaggctttgc tctgttgtac atactgctgg
      841 ttataaccag ttccaggctt gccttgagtc agtttctcta attcggcaat ggcgagtttg
      901 gcataggtca acgccttttc ctggtcagtt cgggcaccag ccgtgaggga ccaggcaagt
      961 tcgttgagca atccgacttg tcgcggttca atggccagga tttgctttac cgtagcttct
     1021 tgtttggccg tgtctttggc ttctttatac aatctgacca atcgttccaa caggtaagcc
     1081 cgaaattgct catcatcggg attttcctgg ttcagggttt cgagctttgc aattcgatca
     1141 gccagtggtg ttttctgatc tgcttctatc gtctgaatct tccggatcca aaaataccgc
     1201 ctgccggcaa agatgtattg gatcggttct ccagtatctg tgaacatcga aatacccatc
     1261 agcccaggcg gggcacctgg ggtgggaaat ttgtcatcga gttctttggt tatttcctgg
     1321 gcagccgtct ggtctttgag tacaagccag caggcaagtc tgatggccga aagcaggttt
     1381 ggtgaagggg gttcagatgc tttcagcgcg gtgagttcct ggcgcatggc ggctttggct
     1441 ttgtcagaat acccggcttt ggccacgttg accgacatca caccaatggc ggcctcgtaa
     1501 ttttttggat tttgttttaa gagcgactga tagagggcga gggcttcatc aaacttcttt
     1561 aactcctcga cctgaaacgc agccaggttg gcaatcattt catgattatt gggatcaagg
     1621 gcaatggctt ttttgtaagc ttcctcagct tttgccaggt cctctttatg aaatcggtat
     1681 aaatcaatca gggctgattg gcccacgacc gagtctggtg ccacggcacc agctttttca
     1741 agtaattcct ggcgaccttc tccgttgacc agcagaatct gatttcgagc caggatcaac
     1801 ggaaaaaccg gattggtcgg gtctttcgcc attttgtcga gatattcctg acgcaagcgg
     1861 tctggtgaga ccagcattaa ttggatatca acatacagat tgtgaagctt ataagaattt
     1921 gggtcctggt tgagtgctgc ctgaattttc gccagtgcac ttttgtagtc ttgagttttg
     1981 tagagctgat tggcctgatc ggacagtgtt tttgccgcat cagacctggt ttcaagcgtg
     2041 taaactgttg tctgggttgc acttgattgt gccagcaatg ggacggaagc cccccaaagc
     2101 cagattgaaa ggcaacacat caggcacaat ttgatccagt atcggcctgg tttcatcaaa
     2161 agttctccct taatgaattg agaatacaga atgtttgtgg tgactggtga ctggtacttg
     2221 gtgcttggtg cctggtgttt ggtgtttggt acttggtgct taggaaccaa tacattcgaa
     2281 gaacgaagaa caaagaacga agaacaaaga acaaagaacc aggcaccaag caccaggcac
     2341 gaagcacgaa gcacgaaaca ccagtcactc agttccgaaa aaattctcaa ttcagattca
     2401 tttaccgaaa ccgatcactg gctttgatca attcgtcggt gattccgggt tcaaacgcgg
     2461 agtgtccggc atcgggaatc acgtgaaact cagcttccgg ccaggctttg tggagtgccc
     2521 aggcggtcat catcgggcac acaatgtcat agcgcccttg aacgatcacc gctggaatat
     2581 gtcgaatgcg gtcaacatct ttcagcaact gatcttcttc cataaagatg ttgttgacga
     2641 agtaatggca ttcaatccgg gcgatggaga gggctttgtc atcggcgcca aactcggtga
     2701 ccagatccgg gtcgggatag agggtgcagg ttgaaccttc ccagatgctc caaattttgg
     2761 cggcttccag gcggagggct tcatcatcac tggtcaggcg gcggtaatag gcgttcatca
     2821 tatcgccgcg ttctgcttcg gggatgaagt tcaaaaacgg ctcccattga tccgggtaga
     2881 tggccccggc gccatcctga taaaaccagg cgagttcttt tttggtacac aagaaaatcc
     2941 cgcgcagaat gagtgcctga acgcgctccg gatgggtttc cgcataggcc agcgccagcg
     3001 tgctgcccca cgaaccgcca aacaccaccc aggtatcaat gcctaaatgt tcgcggatct
     3061 tttcggtgtc ggccaccaga tcccaggtcg tgttttcttc aagcgaggca aatggaaggc
     3121 tttttcctga tccacgctgg tcaaacaaaa taatgcggta ggcttgcggg tcaaaaaatt
     3181 gccggtgttt gggtgatgtg ccgctgcctg gaccgccgtg taaaaaaacc accgggcgac
     3241 cattggggtt tccgctttgc tcatagtaga gcgtgtgaat gtcagacact ttcagcatcc
     3301 cctgatcata gggttcaatc ggatcataga gcgttcgcat agtgatagac ttcctgttca
     3361 attgtgaaat gtgaggtgac gggtgttgta acacaccgac cgtacaactt gaagaaaagg
     3421 ttttgaactg ctttgatttc acgattctaa atcccaattt tcactcctca accaaatgaa
     3481 ccatcatcag cttgaatgga gtcatcgtgg tttttttgtc atccggcagt tgctgacccg
     3541 gcttgatcaa atatttttaa aacaggtgtg tgaccggatt ttcaaccagt ggcttgctga
     3601 atcgccagac ccaactgaag ccgccaactc cacgaatctg gcctatttaa ccgagccgcg
     3661 ctatttcagc caccatcccc aggaactggt ctggctgtta gagttgatcg caagtgaaac
     3721 tgtatttgga ccggtgcagt cggtttgcga ccgggaactg atctttcaca acacccagta
     3781 tttctttgaa ccggccaccc acactcgcga cggtaactgg catcgggatc aacaatttgg
     3841 cgccaccgat gaagccgagg aacaacgctg gatggccacc acgattggcg ttcatattca
     3901 tattgccctc gaacctgacg accacctcga aattgtgcct ggcaccaacg cccgatggga
     3961 taccccggca gaacgcagca ttcgcaaagg gttaaacgga attgcccgaa atgctccgga
     4021 tatgcccggc gccgttagaa ttgaccttca accaggggat gcctgtgtct tcaatgcctg
     4081 gagtatccat cgagggcggt atgtcgcggg taaaccacgt cggacgtttg atgtgattta
     4141 tggaacgaaa cctgactcgt acgtgacacc tccgacgtgt ttccttgacc cgaaacacct
     4201 ggatgggata agtcaatccg cacacctgtt ttaccagaaa aaaatgcgag aaagccccat
     4261 cctcagccgc gaagcggcag ggcggggatg aatcgcagcc gctgaaaaaa accagcctga
     4321 acgaccttaa acaaagtaat aaatcttgcc gaaaacggtg ggttggtgtt agagttaaac
     4381 tatgaggaaa aaagcagata agcccaaaca accccaaacg ccatcctttg tcgccacgtt
     4441 tgaattggag gtggatt
//
LOCUS       DAOQDO010000191         4442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13286_length_4442_cov_3.652838, whole genome shotgun sequence.
ACCESSION   DAOQDO010000191 DAOQDO010000000
VERSION     DAOQDO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13286_length_4442_cov_3.652838"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..486
                     /locus_tag="PKZ53_04285"
     CDS             55..486
                     /locus_tag="PKZ53_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNJ39685.1"
                     /translation="MSEPESPNPIPQDSPATDPIAVQRRLERHIPLVSLMLILIGVWK
                     GGGLLALSVLLGCGLSYLNYRWMSASLRHILATTKDKPSARQMSKFLSRWVVIGVVMV
                     VASKLFYFSMLGILIGLFAFPIAVMLEGAYQAMWVAINGLD"
     gene            523..1374
                     /gene="atpB"
                     /locus_tag="PKZ53_04290"
     CDS             523..1374
                     /gene="atpB"
                     /locus_tag="PKZ53_04290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNJ39686.1"
                     /translation="MYSLLSFFFFLSGAAETAAEHGESAGGHAHQPWFAEQIYHALHL
                     TPEQLPPNIIHALISALICIVGLKLLIGKPSVDKPGAGQQMIEVVVLQVRSMLEQGIG
                     AYGFKYLSFLLPLTFFVLISNLMGLIPIFEPPTNKLSTTLALGLCSFCYYIYMGFSQQ
                     GVGYLKHFTAGLTSGIFAIIGILIFFVEIFSNCLRPITLGVRLMINMFADEQVLDGFG
                     AIVPIVIPSALLVLGTFVCFVQTFIFIQLSIVYLGETVPHDDHHDEEHGHDEEHGHDE
                     GKLAHAH"
     gene            1422..1625
                     /locus_tag="PKZ53_04295"
     CDS             1422..1625
                     /locus_tag="PKZ53_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39687.1"
                     /translation="MNTKDKAKEQRIQRLLMIVVLVQGVAIAVFIGWRLQLSLYLVVP
                     VALVVLIAVLVGMQTAAKQFEDS"
     gene            1940..2257
                     /locus_tag="PKZ53_04300"
     CDS             1940..2257
                     /locus_tag="PKZ53_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNJ39688.1"
                     /translation="MRNLKFKALMLMATLVVFSGPVLAQEAAKSTQFSFGEKKMGLGA
                     LAVAIAAFAGALGDGRAIAAACEGTARNPGAGGRIFTAMVLGLALIETLTLVTFAAAF
                     LGL"
     gene            complement(2342..3202)
                     /locus_tag="PKZ53_04305"
     CDS             complement(2342..3202)
                     /locus_tag="PKZ53_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HNJ39689.1"
                     /translation="MMSRNQRAKLAQETLDILKTGFYQTAAGNSVPLADQQAQSVAGT
                     VVYRPDGWKRVLRKVDQTFLEVPSSVETKIEVTQETTMEAAQRVIQREQAAHVAALNF
                     ASAKNPGGGFLNGSQAQEECLARSSGLYPTLTTTMEMYQHNQLFGSLLYSEYLMYSPN
                     VPFFRDDEGRLLENPYLLSIITAPAPNAGAIQQNQPKSVAKIQEVMFGRMENILAVAF
                     LQGQTTLILGAWGCGVFRNDPKIVATLFAKHLLTGGKFQNRFERVVFAVYATPKEQHN
                     FLTFQSVFGK"
     gene            complement(3270..4025)
                     /gene="sdhB"
                     /locus_tag="PKZ53_04310"
     CDS             complement(3270..4025)
                     /gene="sdhB"
                     /locus_tag="PKZ53_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNJ39690.1"
                     /translation="MDPQARKVIFHIKRQDDPQSQEWWETFAVYYRPRMTVLSCLAEI
                     RTHPFNAQNQKSTPVAWESGCLENQCGACSMLINGRPQQACMARVDTLPQPIVLEPLS
                     KFAVIRDLVVDRSRLFQQSKAAKAWIPTDGVTSIGPVPSGNPDEAELRAHLSRCQACG
                     ICLEVCPQYNDRSPFIGPALLNQLRQLNHHPIGKQNRNERLDLIMGEGGVADCGNAQN
                     CVKACPKEIPLIESIAELNRETTWRTFFRWLSQ"
     gene            complement(4105..>4442)
                     /locus_tag="PKZ53_04315"
     CDS             complement(4105..>4442)
                     /locus_tag="PKZ53_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ39691.1"
                     /translation="RQENKMLLMDFTGSDWCSWCIKLDKEIFSTNEFKSYASKNLVLL
                     KLDFPKSKQLPVDEIRQNQLLAQTYQIQGYPTVIVLNSSGAKVGTLGYMRGGPSEFIA
                     SLDKIRQGA"
BASE COUNT         1022 a         1074 c         1125 g         1221 t
ORIGIN      
        1 cacttctcaa ttttcaatta accacaggcg gcagcccatt ttcaattttc acttgtgtca
       61 gaacctgaat cacccaaccc gatcccgcaa gattcacctg caaccgatcc aatcgctgtg
      121 caacggcgac tggagcgtca tatcccactc gtttcactga tgttgatttt gattggggtg
      181 tggaaaggcg gcggtcttct ggccctgagc gtcttgctcg gatgtggttt gtcgtacctc
      241 aattaccgct ggatgtcggc cagcctccgg cacattctgg ccacgacaaa agacaaaccc
      301 tcggccagac agatgtcgaa atttttatca cgctgggttg tgatcggagt ggtgatggtt
      361 gtggcgtcta agctctttta tttctcgatg ctgggaattc tgattgggct gtttgctttt
      421 ccgattgccg tgatgctcga aggggcctat caggcaatgt gggtggcaat caacggattg
      481 gattaaccca aaccaccagt ccaccgtaag gacacacgta ctatgtactc gcttttgtca
      541 tttttcttct ttttgtctgg agccgctgaa acagcggctg aacatggtga aagtgccggg
      601 ggacatgcac atcaaccctg gttcgctgaa caaatttatc acgccctcca tttgaccccg
      661 gagcaactcc ctcccaatat cattcacgcc ctgatttcgg cgttgatttg catcgttgga
      721 ctcaagcttt taattgggaa gccatcggtt gataagccag gcgccgggca gcaaatgatt
      781 gaagtcgtgg tgttacaggt tcgcagcatg ctggagcagg gaattggtgc ctatggcttc
      841 aagtatctct cgttcctgct gccgctcacg ttctttgttt tgatttcaaa cctgatgggg
      901 ctgattccaa tttttgagcc accgaccaac aaactgagca ccacgctggc gttggggctg
      961 tgctcatttt gttactacat ctacatgggg ttcagtcagc aaggtgttgg gtacctcaag
     1021 cacttcactg ccgggttgac ttcagggatt tttgccatca tcggtatctt gattttcttt
     1081 gtggaaatct tcagtaactg tttgcgtcca attacacttg gcgtccgtct gatgatcaac
     1141 atgtttgctg acgaacaggt cctcgacggg tttggcgcga ttgtcccaat tgtgattcca
     1201 agcgccttgc tcgtccttgg cacgtttgtt tgctttgtgc agaccttcat tttcatccag
     1261 ctttcgattg tgtatctggg tgagacggtg ccgcacgacg accatcacga tgaagaacat
     1321 ggtcatgacg aagaacacgg ccatgacgaa ggcaaactgg ctcacgccca ctgatttctc
     1381 gtaacaaatc acctgtcatc ctggatcagg tacgttgttt gatgaacacg aaagacaaag
     1441 ccaaagaaca acgaatccaa cgtcttttga tgatcgtggt gctggtacag ggggttgcca
     1501 tcgccgtttt cattgggtgg cgactgcaac tcagccttta tcttgttgtc ccagttgcgc
     1561 ttgttgtctt gattgccgtt ctggttggta tgcaaactgc agcaaaacag tttgaagatt
     1621 cttgaaacgc tcttttcatt gtggctattt ctgatcggca ggttgttcac cttttatatc
     1681 ttttccacca gttgtcgctt ttggagcctt gctgtacggg ttgtaaggga taccgggcaa
     1741 ctgggctatc ataccgtgaa cattttgttg agctgaatgc gcctcccact ggctgtcccg
     1801 tcggcagcct ttcaactctg acttttggga acaaagggag aggaagccag aatcattgag
     1861 aaatccagac actaacgtat caatcgtttc tgaaccccgc ccgcaatccc gcaaccctta
     1921 tctggaggat attccgttca tgcgtaactt gaaattcaaa gctttgatgt tgatggccac
     1981 cctggtggtc ttttctggtc cagtgctcgc ccaggaagcg gccaaatcaa cccagttctc
     2041 ctttggtgaa aagaaaatgg gccttggtgc tctggccgtg gcgattgctg cgttcgcggg
     2101 tgctctgggc gacggtcgtg cgattgcggc tgcctgcgaa ggcacagctc gcaacccagg
     2161 agctggtggt cggatcttca ccgccatggt gctcggtctg gctctgatcg aaacgttgac
     2221 cctggtgacc tttgctgccg cgttcctcgg cctgtaattt ccaatttcca gtttgagaac
     2281 gccaaaaagg gcgcagagta aaacgactct gcgccctttg ttttttgctt ttcgtcagag
     2341 gctatttccc aaagactgat tggaaggtca ggaaattatg ctgttccttc ggggttgcat
     2401 ataccgcaaa cacaacccgt tcaaatcggt tctgaaattt cccacctgtc agcaggtgtt
     2461 tggcaaagag tgttgcgacg atctttgggt cattgcgaaa gacaccacat ccccaggcac
     2521 ctaaaatcag tgttgtttga ccttgcagga acgcgaccgc caggatgttt tccattcggc
     2581 caaacatgac ttcctgaatt ttagcaacac ttttgggttg attctgctga atggctccgg
     2641 catttggcgc cggggcggtg atgattgaaa gcaagtaggg attttcaagc aaccgccctt
     2701 catcatctcg gaaaaagggg acattcggcg aatacatcaa gtattccgag tagagaagcg
     2761 acccgaacaa ctggttatgc tggtacattt ccatggttgt cgtcagagtt ggatacagcc
     2821 cagatgaccg tgccaggcat tcttcttgtg cctgagaccc attcaaaaaa cccccacctg
     2881 gatttttcgc tgaagcaaaa ttgagtgctg ctacgtgagc ggcctgttca cgctggatca
     2941 ctcgttgagc cgcctccatt gtggtttcct gcgtcacttc aattttggtt tcgaccgaag
     3001 atggtacttc caaaaaggtt tgatccactt tccgaagaac ccgtttccat ccgtcagggc
     3061 ggtacacaac tgttccggcg actgattgag cttgttggtc tgccagaggg actgagttcc
     3121 cggcggcagt ttggtaaaaa ccagttttaa gaatgtcgag ggtttcctgg gcaagcttgg
     3181 cgcgttggtt tcgactcatc ataagcacaa gggaaaaagg caaaaatcaa aaaagaaggg
     3241 tgcttcaagt cagattgatc gctggtgggc tactgactga gccatcgaaa aaaggtacgc
     3301 caggtggttt cccggtttaa ttcggcaatt gactcaatga gtggaatctc ttttggacag
     3361 gctttgacac aattttgggc attgccacaa tctgcgaccc ctccttcacc catgatcaga
     3421 tcaagtcgct cattgcggtt ttgtttcccg attgggtgat ggttgagttg ccgtaactga
     3481 ttgagcaggg ccgggccaat gaatggggat cggtcattgt attgagggca cacttcgagg
     3541 caaatgccgc aggcctggca gcgtgaaaga tgagcccgta attctgcttc atccgggtta
     3601 cccgatggaa ccggcccgat ggaagtgaca ccatccgtcg ggatccaggc tttcgcagct
     3661 ttagattgtt ggaacaaccg gcttcgatca accaccagat cacgaataac tgcaaatttg
     3721 ctgagtggtt ccagcacaat cggttgtggc aaggtatcaa ctctggccat gcaggcttgc
     3781 tggggacggc cattgatgag catcgaacag gcgccgcact ggttttcaag acatccggat
     3841 tcccaggcaa caggggtaga tttctggttc tgggcattaa atggatgggt gcgaatttca
     3901 gccagacacg aaagaacggt cattcgaggc cggtaataga ccgcaaatgt ttcccaccac
     3961 tcctgacttt gtgggtcatc ctgccgcttg atgtgaaaaa taacttttcg agcctgtgga
     4021 tccataaaaa agaaaagagt tggaattttg aactattcga atttcaaaac ccaactcagt
     4081 cagaaaatga atggagtgaa tttgttatgc gccttgtcga attttatcga gcgaagcaat
     4141 aaattctgag gggccaccac gcatataccc aagcgtaccc acctttgcgc cacttgagtt
     4201 taaaacgatc accgttggat agccttgaat ctgataggtt tgagccagta actgattttg
     4261 gcggatttca tcaactggca actgcttcga tttaggaaaa tcaagcttga gtaagaccag
     4321 gtttttactg gcatatgatt taaattcgtt ggttgagaag atctctttat caagtttgat
     4381 gcaccaggaa caccagtctg agccggtaaa gtccatgagc agcattttat tttcttgcct
     4441 gg
//
LOCUS       DAOQDO010000192         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13296_length_4441_cov_2.655951, whole genome shotgun sequence.
ACCESSION   DAOQDO010000192 DAOQDO010000000
VERSION     DAOQDO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13296_length_4441_cov_2.655951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /gene="pilB"
                     /locus_tag="PKZ53_04320"
     CDS             complement(<1..1744)
                     /gene="pilB"
                     /locus_tag="PKZ53_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNJ39692.1"
                     /translation="MSAKLGEILLKENLLTPQQLKEALDYQRAHGGRLGSILVNLKLV
                     PDEEITSILSRQYGVPAVNLDLFDVDPNAVKIIPEETARKYMVLPLARVGPTLTLAMV
                     DPANVFAIDDIKFMTGLSIEQVVVSEIAMLKALDKYYAPERGLELASQMNQFDQQFMG
                     GGAAAAAAAGPSGPSLADQMSEFVDDEATNLEVLEEQDEINLDDSEKGANEAPVVKLV
                     NLVLVNSLEYGASDIHIEPYEKEFRIRFRIDGILKEIMRPPQKMRDALTSRIKIMSKL
                     DIAEKRLPQDGRIKIKLRREGRARDLDFRVSTLPTLWGEKIVMRLLDKEKLMLDMTKL
                     GFEVQSLERFKRQIAKPYGMVLVTGPTGSGKTNTLYSALASLNTPDTNIMTAEDPVEF
                     NLTGINQVQMKEQIGLNFAATLRSFLRQDPNIILVGEIRDFETAEIAVKAALTGHLVL
                     STLHTNDAPSTVSRLMNMGIEPFLVATSVNLIQAQRLIRRICTECKAPAKVQPPLQTL
                     LEVGFTPEDASNITIVEGHGRLADGRECPKCKGSGYKGRVGLYEVMEMTDELRELILV
                     GASALEIKKKAMEDK"
     gene            2053..>4441
                     /locus_tag="PKZ53_04325"
     CDS             2053..>4441
                     /locus_tag="PKZ53_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNJ39693.1"
                     /translation="MVLQQDWELVWTRRPLWPASWAAALGIGWGSGQGTLSVVLWLMV
                     MASLGGIISYLKSWSGLMWSSVAMVVFGAGALCAIQEHQVMTSLTQLRLVLTQHQIQP
                     DEAIRLTGWVEDWPEPGVERLYLTLNVTEVDCKDQVLPAQGRLSLLFPLEKTQFKHQW
                     AAHPLVPGTLLRLVARVQWQTEYQNPGMLELDDVLRTLNLSAVGRIHSIESIITPGTD
                     QGWLVRMRLLRLRAIHRLFLDFEPRVAGLLAALLFGSRAFLDAGWAESFRQTGTFHLL
                     VISGSHLALLGWFVASITRRWFSSRWVSASITLAFIWSYCAMVGSEPPVLRAAVAITV
                     WQLCGLFFRQPEPLNVLGAAILVLLFLKPSDLFSPGFQLTIGAVWMLVGGVMPLVSRL
                     EQIGNWRPVRQTPLPPMCPTWIRTLAEALYWSPQQFAEDMNRSPVTYQLEKSKAAFRL
                     EQIGLPQFLRKRFGWAKMMNLQVLIRWGVVLLIASGMVQLALLPLSAAYFNRFTPIGL
                     ISNLGAEVVLGFLLILASGYFLSLIVDAGLTSALILALDWATSGFVKLATWGAGWSWA
                     SWRIPHLEGQLIGVYVLYGVVMMVLIASLQHWKPLWPAPRPEPAFGKKYWVTLAVLLV
                     VIWGMLAVWIINPPRWWTAAPAGVLRVTFLDVGQGDSAVIEFPTGQVMLIDSGGWSAW
                     TRPVESGFNPDRRGIGERVVSRALWARGIRRLDWIVGTHGDADHLQGFTEVIRNFPVG
                     QALGSGCKSPDQTYRTWQQALDTQHLQLIQLGAGYRWDIGAVKVEVLSPPSVSEPL"
BASE COUNT          912 a         1108 c         1259 g         1162 t
ORIGIN      
        1 gcttgtcttc catggccttc ttcttaattt caagggctga agcgccgacc agaatcagtt
       61 cgcgaagctc atcggtcatt tccatgactt catagagtcc aacgcgtcct ttataaccgc
      121 ttcccttaca tttcgggcac tcccggccat cggctaaccg accgtggcct tccacaatcg
      181 tgatattgga ggcatcttcg ggtgtaaatc caacttcaag cagggtttgg agtggaggtt
      241 gaactttggc tggggctttg cattcggtac aaattcggcg aatcaatcgc tgggcttgaa
      301 tgagattgac tgaggttgca accagaaatg gctcaatccc catgttcatc aagcggctga
      361 ccgtagatgg ggcgtcattg gtgtgcagtg ttgagagcac caggtgcccc gtcaacgccg
      421 ctttgaccgc aatttcagcc gtttcaaagt ctcggatctc tccgaccaga ataatgtttg
      481 gatcctgccg gagaaacgac cgcaatgtgg ctgcaaaatt taagccgatt tgttccttca
      541 tctggacctg attgatgccg gtcaggttga attcaaccgg atcttcagcc gtcatgatgt
      601 tggtatcggg ggtgtttaag ctggcaaggg ccgaatagag cgtgttggtt ttgccagacc
      661 cggtggggcc tgttaccagg accatgccgt acggcttggc aatttgacgc ttgaatcgtt
      721 ccagactctg gacctcaaac ccaagcttgg tcatatcaag catgagtttt tctttgtcaa
      781 gcaaccgcat gacgatcttt tcgccccaga gggtcggtag ggtcgaaacc cggaaatcaa
      841 ggtcacgtgc ccggccttca cggcggagtt taatcttgat acgaccgtcc tgggggaggc
      901 gcttttcggc aatgtccagt ttggacatga tcttgatacg ggaggtgagc gcatctcgca
      961 ttttttgcgg aggccgcata atttccttca agatgccgtc aatgcggaac cgaatccgga
     1021 actccttttc atagggctca atatgaatgt cactggcacc gtattcgagt gagttgacca
     1081 aaacgaggtt gacgagtttc acaaccgggg cttcattggc acctttttca gaatcgtcaa
     1141 ggttgatttc gtcttgttct tccaaaacct caaggttggt cgcctcatca tcaacaaact
     1201 cgctcatttg atcagccagt gatggtccac tcggaccagc agcggcggcg gcggcggcac
     1261 ctccacccat aaattgctgg tcaaactggt tcatttgact ggccagttcc agcccgcgct
     1321 ctggggcata gtacttgtcg agcgctttga gcatggcaat ttcagaaacc accacctgct
     1381 caatactgag cccggtcata aatttaatgt cgtcaatggc aaacacatta gccggatcaa
     1441 ccatggccag cgtgagcgtt ggtccgacac gcgcgagcgg aagcaccata tacttgcggg
     1501 cggtttcttc agggatgatt ttgacggcat tgggatcaac atcaaaaaga tctaaattaa
     1561 cagccgggac cccatactga cggctcagaa tagatgtaat ctcttcatct ggaaccagtt
     1621 tcaggttgac cagaattgaa ccaagccgcc caccatgggc acgctggtaa tccagagctt
     1681 ctttcagttg ttgcggagtg agtaaattct ctttgagtag gatctcgcca agcttcgcgg
     1741 acatgcgtat gtttccccga tagagaaaag gataacctag cggctaagtg ttgtcgttgg
     1801 actgtttttt ggaaaaccag gtgatagtag aggctttata gaaaaagagc agcaaccaaa
     1861 aggacagggc cagaatagtg caacgtcatg aagactgtca agcatcgcgt ctgattgccg
     1921 gattttcctg gtgaaaaccg tgatttatcc ggtttttttt caggtcagtt cggaacactt
     1981 tttgccctct cttctccagt ttaataccaa tttcgattct gtgagaaatt taattttgag
     2041 agggcaatgt gcatggtttt gcagcaagac tgggaactgg tttggacccg gcggccatta
     2101 tggcctgcga gttgggcggc ggctttgggg attgggtggg gctcaggtca gggaacgttg
     2161 tctgtggtgt tatggctgat ggtaatggcg agcctcggcg gtatcatcag ttacctgaaa
     2221 tcctggtctg gtttgatgtg gagtagcgtt gccatggtgg tatttggggc tggcgcgctc
     2281 tgtgccattc aagaacacca ggtaatgact tccctcactc aactgaggct ggttttgact
     2341 caacaccaga ttcaacccga tgaagcgatc cggctgactg gatgggtcga agattggcca
     2401 gagcctggtg ttgagcgtct gtaccttacc ctgaacgtga ctgaggtgga ctgcaaggac
     2461 caggtgctgc cggcccaggg gcgactgagc cttctcttcc cgctggaaaa aacacaattc
     2521 aagcaccaat gggcggccca tccactcgtg cctggcaccc tgctgcgtct ggttgccaga
     2581 gtacagtggc agactgagta ccagaatcct ggaatgcttg aactcgatga cgttctccgt
     2641 actttgaatc tgtctgccgt tggccggatc cactccattg aatccatcat cacgccaggc
     2701 accgaccagg ggtggcttgt gcggatgaga ttgctcagat tacgggccat tcatcgtctg
     2761 tttcttgact ttgaaccacg agttgccggg ttgctggcgg ccttactctt tggcagtcgg
     2821 gcctttctgg atgccggatg ggccgagtcc tttcgacaaa ctggaacctt ccatttgctg
     2881 gtgatctcgg gctcgcatct ggcgctcctt ggctggtttg tggccagcat cactcgccgc
     2941 tggttttcct cgcgttgggt gtctgcctcg atcaccctgg ccttcatctg gagctattgc
     3001 gccatggttg gaagcgaacc accagtcctg cgggcagcgg tggcgattac ggtctggcaa
     3061 ctgtgcggac ttttttttcg tcaacctgaa ccactcaatg tgctgggcgc cgcaattctg
     3121 gttctgttgt ttttgaaacc gtcagatctt ttttcacccg gcttccagtt gaccattggc
     3181 gcggtctgga tgctggttgg tggtgtgatg ccactggttt cacgtcttga acagattggg
     3241 aactggcgac cagttcgtca gacacccttg ccgccgatgt gcccgacgtg gattcggacc
     3301 ctggccgaag cgctctactg gagcccccag caatttgccg aggatatgaa ccggtctccc
     3361 gtgacctacc agcttgaaaa atcaaaagcc gcgttccggc ttgagcaaat cggccttccc
     3421 cagtttctca gaaagcgatt tggctgggca aaaatgatga atctccaggt tttgatccgg
     3481 tggggcgtgg tgctgttgat tgcttcagga atggttcaac ttgcgttgtt gccactcagt
     3541 gccgcctact ttaaccgatt caccccgatt ggcctgattt ccaatttggg ggcggaggtg
     3601 gtgcttgggt ttcttctgat tctggccagc gggtattttc tttcactgat tgttgatgca
     3661 gggctcacca gcgctttgat cctggcgttg gattgggcga catcaggctt tgtcaaactg
     3721 gccacctggg gggccggatg gtcctgggca agctggcgca ttccacacct ggaagggcaa
     3781 ttgattggtg tgtatgtcct gtatggagtc gtgatgatgg tgttgattgc cagccttcag
     3841 cactggaagc cgctctggcc agctcccagg ccagagccag ccttcgggaa aaaatattgg
     3901 gtcaccctgg cggtgttgct ggtggttatc tggggaatgc tggccgtctg gattatcaac
     3961 ccaccgcgct ggtggacagc cgctccggcg ggtgtgttgc gagtaacctt tctcgatgtc
     4021 gggcagggag attcagccgt gattgaattt ccaaccgggc aggtgatgtt gattgacagt
     4081 ggcggctggt cagcctggac cagaccggtt gaatcgggat ttaaccctga ccggcgggga
     4141 attggcgagc gggttgtttc acgggcattg tgggcacggg gaattcgtcg gctggattgg
     4201 attgtcggaa cgcacgggga tgctgaccat cttcaaggat ttaccgaggt cattcggaat
     4261 tttccggttg ggcaagccct ggggagtggt tgtaagtcgc cggatcagac ttaccgaacc
     4321 tggcaacagg cgctcgacac acagcacctt caactgattc aactgggcgc tggatatcgc
     4381 tgggacatcg gggcggtgaa ggttgaggtt ctttcgcctc catctgtatc tgaaccatta
     4441 t
//
LOCUS       DAOQDO010000193         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13332_length_4433_cov_2.617862, whole genome shotgun sequence.
ACCESSION   DAOQDO010000193 DAOQDO010000000
VERSION     DAOQDO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13332_length_4433_cov_2.617862"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3626
                     /locus_tag="PKZ53_04330"
     CDS             <1..3626
                     /locus_tag="PKZ53_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39694.1"
                     /translation="WGPRGVVQTPDGMLYITDADNHVIWKAQIEGTKSLSVIAGGVGV
                     AGLLDGPGNTARFNGPQDLVIGTTPNVLVVSDRGNRVVRLVTLPTGEVLTVGAIPAAR
                     QSSTSSEPQQNPAGVAFTDPSGLDVDLQGNIYVADGNSVKIVGSQGQNLAVAELAQTG
                     TFQNAVAIAISGATAYVLDAGRQQVIRVGFQGTPTITSITPNQVKLGENTEVKIRGTN
                     FLSETQVLIGGIPAREVDVESATEIELRLPPINQAGTYPLVVSHRGGRVESTITIGES
                     ASFGITASPNEVELVAGTTRQITINLQRRNFTGPVTLQLDRQLLVGVAATFSPNIVTG
                     NSATLTLSALPGIIPGEFKFQVLGTAPGLVASTEIEVKVRPAQAGQVQLSASPVSQTL
                     RAGEQTRFTIFLNRDNFVGEVELEVEDEPDGIEARLQPETVTGNVATLEIETRRDLSP
                     GTYPLLIKGSADGAAITPTRVQISIENSIDAGTIVLSASPVSQAVPPGGSVRYDIFVN
                     RASYSGEVRLSVSGVPAGIEAEFDDDSTDANQTTLHLAASGSVTPGAYVLIIEGDAGE
                     AFVQSTQVELIVESGGGGNTSVSLSASPASQTIQRGGEGRYIIFLNRNGYFGEVNLSV
                     TGAPAGVEAELDPDSVFGDQTTLFVSAESEAIPGTYVLTIEGSSNRFQVTPLQVMLIV
                     EGGGGGSNGSVRISASPVLQSIRPGDSTQYTLFLTRINFNDEVNLTVTGAPFGVEAEV
                     DPDSVEGNTTQLMVTSDNSVVPGTYTLQIEGSAPGATVIGTQVQLVIGNGGGGTGGSV
                     SLAASPTSQTIRPGENTSYTIFLNRTNFSGEVNLTVTGAPFGVEAEADPDSVFGDSTT
                     LNVFTETSAPPGTYTLRITGTASGATVIATQVQLTIGNGGGGTGGTVTLSASPGSQTI
                     APGNSTSYTIFLNRTNFSGEVNVTVTGAPFGVEAEADPDSVFGDSTTLNVVTETNAPP
                     GTYTLRIEGTATGATVIATQVQLIIGNGGGGTGGTVTLSASPGSQTIPQGEDAGYTIF
                     ISRSDFSGTVTLGVSGVPDGVTAEFSPESTTGNSSVLTVLVDASAQPGTYILTIEGAA
                     SEITIDPIQVELVIPGESNVRQVILSASPASQTVNVGGSTTYTVFLTRTNVTGEVNLF
                     VDNVPDGVEAEFTNDSPTGNTTQLQISVENSAEPGTYVLIIGGEATGAEVIHTQVRLI
                     LQR"
     gene            complement(3744..>4433)
                     /locus_tag="PKZ53_04335"
     CDS             complement(3744..>4433)
                     /locus_tag="PKZ53_04335"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ39695.1"
                     /translation="DLVHRLRRCGSRVVCAGRNEVRLNALAEESGAEAFILDATQTSQ
                     VEGCISQVVDKYGRIDGIANCVGSLLLKPAHLTTDAEWSTTLATNLNSAFAVVRAGAK
                     YMKNGGSVVLVSSAAARIGLANHEAIAAAKAGIIGLALSAAASYAAKGIRFNCVAPGL
                     VQTPLTERITSNELALKGSVAMHALGRIGDPSDVASAIEWLLDPQNTWVTGQVLAVDG
                     GLSSIRPRSNH"
BASE COUNT         1072 a         1140 c         1189 g         1032 t
ORIGIN      
        1 cgtgggggcc acgcggcgtg gtgcaaactc cagatggaat gctctatatc acggacgctg
       61 ataatcacgt tatctggaaa gctcagattg aaggtacaaa aagtttgtcg gttatcgctg
      121 gtggcgtcgg tgtggccggg ttgctggatg gccctggaaa taccgcccgg tttaacggcc
      181 cccaggatct ggtgattggc accaccccaa atgtgctggt ggtctcagat cgtgggaaca
      241 gagtggtccg actggttacc ttgccaactg gggaagtcct gaccgttggt gcgatccccg
      301 ctgctcgcca atccagtacc agttctgagc cccaacagaa tccagccgga gttgcgttta
      361 ctgaccccag cggtctcgat gttgatttac aggggaatat ttatgttgcc gacgggaaca
      421 gcgtcaaaat cgtaggctca cagggacaga atctggcagt ggccgaactg gcccaaaccg
      481 gaacattcca aaatgcagtg gcgattgcca tttcaggtgc cactgcctat gtgctcgatg
      541 cgggtcgcca gcaggtcatt cgagttggat ttcagggaac cccaaccatc acctcaatta
      601 ccccaaatca agtaaaacta ggtgaaaata cagaggttaa aatccggggg acgaactttt
      661 tatccgaaac ccaggtgctg atcggcggca ttcctgcgcg cgaagttgat gttgaaagcg
      721 caactgagat tgaacttcgc cttcctccga tcaatcaggc cgggacctat cccctggttg
      781 tcagccaccg tggcggacgg gttgaaagca cgattacgat tggcgaatca gcttcatttg
      841 gaattacggc gtcacccaat gaagttgagc tggttgctgg aaccacgcgg cagatcacga
      901 ttaaccttca gcgccggaat ttcaccggcc cggttactct ccagcttgac cgtcagttgc
      961 tcgttggcgt tgcggcgaca ttttcaccaa atattgtgac cggaaacagc gccaccctga
     1021 ccctttcggc attgccaggg attattccag gtgaattcaa gttccaggtt ttgggaacgg
     1081 cgccgggact ggtggcctca actgaaatcg aagtaaaagt tagaccggcc caggctggac
     1141 aggttcaact ttctgcttca cccgtcagcc agacgcttcg agccggtgaa cagacacgat
     1201 ttacaatttt cctgaaccgt gacaattttg tcggtgaagt tgaacttgag gtcgaagatg
     1261 agcccgatgg gattgaggcc cgtctccagc cagaaacagt caccggtaat gtggcgaccc
     1321 tcgaaattga gacccgtcgc gatctctctc cgggcaccta cccgctcctg atcaaaggct
     1381 ctgccgatgg tgccgcgatt actccaactc gcgttcagat cagtattgaa aactcaattg
     1441 atgctggcac gattgtgctc tcagcctcac cagtttccca ggcggtgccg ccgggtggat
     1501 cggtacgcta tgacatcttt gtcaatcggg ccagttatag tggcgaggtt cggttgtcag
     1561 tcagcggcgt gcctgcggga attgaagctg agtttgatga tgacagcacc gatgccaatc
     1621 agaccacatt gcatcttgct gcctctggca gtgttacacc cggtgcctat gtccttatta
     1681 ttgaagggga tgccggagag gcttttgttc aatcaaccca ggttgagttg attgttgaaa
     1741 gcggcggtgg tggaaacaca tccgtgagtt tgtcagcttc acctgcctct cagaccattc
     1801 aacgcggagg agaaggccgg tatataatct tcttaaatcg gaacgggtat tttggcgaag
     1861 tgaatctctc ggtcacgggg gcaccggctg gggtcgaagc tgaacttgac ccggatagcg
     1921 tgtttggtga ccagaccaca ctgtttgtca gtgccgagtc agaagctatt cccggaacct
     1981 atgtcttaac catcgaggga agctcaaacc gctttcaggt aacaccgctt caggtcatgt
     2041 tgattgtgga aggtggtggc ggaggttcga atggttcggt tcggatttca gcttcgcctg
     2101 tgctgcaatc tatccgacca ggcgatagca cccagtacac cctttttctc acccgcatca
     2161 attttaatga tgaggtgaat ttgactgtca ccggggcccc ctttggggtt gaagcggaag
     2221 ttgatccaga tagcgttgaa gggaatacca cccaactgat ggtgaccagt gacaacagtg
     2281 ttgtacctgg aacctatacg ttacagattg aaggctcggc gcctggtgcc actgtgattg
     2341 ggacacaggt gcaactggtt attggaaatg gcggtggcgg aacaggtggt tcggtctcgc
     2401 ttgcagcctc accaaccagt caaaccattc gacctggaga aaataccagt tacacaatct
     2461 ttttgaatcg caccaacttt tcaggcgaag tcaatctcac cgtgacaggt gcgccatttg
     2521 gggtcgaagc cgaagccgac ccggatagtg tgtttgggga ttcaactacg ttgaacgtgt
     2581 ttaccgaaac cagcgcccca ccagggacct acacgcttcg aattaccggg accgccagtg
     2641 gtgctaccgt cattgccacc caggttcaat tgaccattgg gaacggtggc ggtgggacgg
     2701 gtggaacagt gacactttcg gcttcaccag gcagccaaac aattgcacct ggaaatagta
     2761 ccagttacac gatctttttg aatcgcacca acttctcagg ggaagtcaat gtaacggtga
     2821 caggtgcgcc atttggggtc gaagccgaag ccgacccgga tagtgtgttt ggggattcaa
     2881 ccaccttgaa cgtggttacc gaaaccaacg ccccaccagg gacctatact cttcggattg
     2941 aaggaacggc cactggggca actgtgattg ccacgcaggt tcaattaatt attggcaatg
     3001 gaggcggtgg gacaggtgga acggtgacac tttcggcttc accaggcagc cagacgattc
     3061 cccagggtga agatgccggt tacacgattt tcatttcacg aagtgatttt tcaggcacag
     3121 ttaccctggg agtttcgggt gttccagatg gtgtaaccgc tgaattttca cctgaaagta
     3181 caactggtaa tagctcggtg ctgacagttc tggttgatgc aagtgcccaa cctggaacct
     3241 atatcctcac cattgagggg gcggccagtg aaatcacgat tgatccaatc caggttgaac
     3301 tcgtgattcc tggtgagtca aatgtgaggc aggtcattct ctcagcctca ccagcttctc
     3361 aaaccgtcaa tgttggtggg tcaacgacct acactgtctt tttgactcga acgaatgtca
     3421 ctggggaagt aaatctgttt gtggataatg ttccggatgg ggttgaggct gaatttacaa
     3481 atgactcacc gaccggaaat accacccagc ttcaaatctc ggttgaaaat agtgcggaac
     3541 caggtaccta tgttttgatc attggtggtg aggcaactgg tgccgaagtc attcacaccc
     3601 aggtaaggtt gattcttcaa cggtgagctt tccagaattt ccagaacaaa aaaggctgcc
     3661 tgaagaaggc agccaaacaa agaaaagaga gagaatcggg agttcaaaaa accgctgaga
     3721 gtaacaacct gaagcaaaaa gcgttagtga tttgagcgtg gacgaatgct ggacaaccct
     3781 ccgtcaacgg ccaggacctg accggtgacc cacgtatttt gcgggtccaa taaccattca
     3841 atggccgaag ccacatcaga tggatcccca atccggccca acgcatgcat cgcgaccgaa
     3901 cctttgagtg ccagctcatt tgatgtaatg cgttctgtca acggtgtttg caccaaccct
     3961 ggtgccacac aattaaaccg aatccctttg gcggcgtatg aagcggctgc cgaaagtgca
     4021 aggccaataa tcccggcttt ggctgcggca atggcttcgt gatttgccag accaatccgg
     4081 gccgccgccg aagaaaccag caccacggat ccgccatttt tcatatactt tgcgccagcg
     4141 cgaaccaccg caaatgccga attcagattt gtcgcaagcg tggtgctcca ctcagcatca
     4201 gtggttaagt gagctggttt taacaacagc gaccccacgc aattggcaat gccgtcaatt
     4261 cgaccatatt tgtcaacaac ttgagaaata caaccttcaa cctgactggt ttgggttgca
     4321 tccaggatga atgcttcagc gccagattct tcggccaacg cattgagtct gacttcattg
     4381 cgaccagcac acacaacccg cgacccacac cgcctgagtc ggtgaaccag gtc
//
LOCUS       DAOQDO010000194         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13342_length_4431_cov_3.316042, whole genome shotgun sequence.
ACCESSION   DAOQDO010000194 DAOQDO010000000
VERSION     DAOQDO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13342_length_4431_cov_3.316042"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="PKZ53_04340"
     CDS             complement(<1..1754)
                     /locus_tag="PKZ53_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39696.1"
                     /translation="MSFPILTGQTAGLNATGGMIARQGTPVLSSISPAQTSKGTVPSK
                     IKIKGSGFVSGSVVKFNSTQITAAKFKDASKIVIKGAPTELFQSDTTYKVTVTNPDGK
                     VSNSIDFVVGAGSSTATATEIEIVDPETLVVNTPNDLTANTISLTVKVLDQNGSPIPN
                     PPITYRSLNPEIASISPTGVITGIAEGAATIELTSGNVVKQVSCAVNQVTTTVSGIFG
                     EADLEIQGSTRRNFASDFQKHTIRAGQTGQALSGFVGKADNPGNNDGDAAGVLLNGPL
                     GLAVGQALYLADVNNQAIRRLDPQTGKTQTLITLADVTRTAGVTNWGPRDISVDSNED
                     LYITDAVNHVIWQADINNTPVKLKVLAGTPGQPGSKDGTGTAAQFTTPQRIDLEGNVL
                     SVTEDSSLVRQIALPGGEVKSISSTSGRNDRPSRSNRQFLQRPKAIASDSAGNLYVVD
                     DRRVRVLSITRDRVSIGDLAPPGTFRNPVAVAVLEDGVFVLDAELGSIVRVKIGVPSI
                     TSISPNPVQSGQVTELIVKGTNLSEPTFITIGRNPFVLARVVSATELRVTVPPIPYGG
                     EFPLIIHTRGGEAIATLKV"
     gene            2215..>4431
                     /locus_tag="PKZ53_04345"
     CDS             2215..>4431
                     /locus_tag="PKZ53_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ39697.1"
                     /translation="MAFEELPSTQIEDIYPLSPLQQGMLFHSLLTPGAGLYIEQMACT
                     LKGPLDVAAFERAWQVLVGRHSILRTAFVWEGLDEPLQVVQSEVEVPFEFLDWRNLTQ
                     SDQEAQLNELLTHQRHQGFELTQAPLMSLTLIQCGGLEFQFIWSHHHLLLDGWGVPIV
                     LREVFTAYEAIRQDRAPALAAVRPYRDYIGWLKEQSVEQAETFWRQNLKGFSAPTYLS
                     AFQTATDTAPRSERCELNLDEPTSQRLATLAREHQITLNTLILGAWAKLMSVHTTADD
                     VVFGVTVSGRPAELPGSDTMLGLFINTLPLRVALPAPIPVTTWLAELQIQQAEMRQFE
                     STPLVNIQGWSEIPRGTALFDTLFIFENYPSPQSLTSGQERLTAISLEKVTARGNTNY
                     PLTFVAAPGNELHFLAVYDQSVFSQAFVTGLMGQMTALLENLAQAPDQSLGEWSPLSE
                     AQRHQILVDWNATARAFPDDTCIHHLFEKQVRQTPDAVAIVFQDQSATYRELNYRANQ
                     LAFYLQLIGVKPETLVGLCVNRSVEMLVGMLGILKAGGAFVPLDPSYPKERLAFMLAD
                     SQVPVLVTQSVLLSRLPRHDSQVVYLDQPLPTISHRPFPVQATSHNLAYVIYTSGSTG
                     TPKGTLLEHQGLCNLALAQIQDMGFAPGSRVVQFSSLSFDASVFEIFATLVGGSTLYL
                     GTRVDLMPGAELAHFLRHHQINVATLPPSAAAALPEGEYPALRTLITAGEPCTAELV"
BASE COUNT         1025 a         1219 c         1093 g         1094 t
ORIGIN      
        1 accttcaggg tagcaatcgc ttcaccaccc cgagtatgaa taatgagagg gaattcaccg
       61 ccataaggaa tcggaggcac cgtaacgcgc aattcggttg cacttaccac cctggccagt
      121 acaaatgggt tccgtccaat tgtaataaaa gtgggttcac tcaaattagt tcctttgaca
      181 attaactcgg ttacctgccc ggattgaacc gggtttggcg aaatgctggt aatgctggga
      241 acgcctattt tcacccgcac aattgaaccc agttcggcgt cgaggacaaa aacaccatct
      301 tccaaaacgg ccacagccac cgggttgcga aaagtgcctg gcggagctaa atcaccgata
      361 ctcacccggt ccctggtgat gctgagcacc cggacccttc ggtcatcaac gacgtataaa
      421 tttccggctg aatcagaggc aattgccttt ggccgttgga gaaactgacg gttggatcgt
      481 gatggtcggt catttcgccc tgaagttgag gaaattgatt tgacttcgcc acccggtaac
      541 gcaatttgcc ggaccaggct gctgtcttcg gtcacactca acacattgcc ttcaagatca
      601 atacgttgtg gcgttgtaaa ctgggccgcg gtgccggtgc catcttttga accgggttga
      661 ccaggcgttc cagccaggac tttgagctta accggagtgt tattgatgtc tgcctgccag
      721 ataacgtgat tgacggcatc ggtaatgtag agatcttcat tggaatcaac ggaaatatcg
      781 cgcggccccc agtttgttac cccagccgtt cgcgtcacgt cggccagggt aatcagagtt
      841 tgggtcttac cagtctgggg atccaatcgt cggatggcct gattgttcac atcggccaga
      901 tacagcgcct gaccaacggc cagcccaagc gggccattca acagtacccc tgcggcatcg
      961 ccatcgttat ttcctggatt atcggccttt ccaacaaaac ctgagagagc ctgccccgtc
     1021 tggccagctc tgatcgtgtg cttttggaaa tcagaggcaa aatttcgccg ggtgctgcct
     1081 tgaatttcca aatcagcttc tccaaaaatc cccgaaacag tggtcgtgac ctgattgaca
     1141 gcacaggaga cttgcttgac gacattccct gaagtcagct caatggtggc tgccccttca
     1201 gcaatgccgg taatcacccc ggttgggcta attgaagcaa tctctgggtt taaacttcgg
     1261 tacgtaatcg gcgggttcgg aatcgggctt ccattctgat caagcacctt tactgtcaac
     1321 gaaatcgtgt tggcggtgag gtcattggga gtgttgacga ccagcgtttc cgggtccacg
     1381 atttcaattt cagttgcagt tgcagttgat gatccggcgc caacgacaaa atcaatgctg
     1441 tttgagacct tcccatccgg gttcgtcaca gttactttat aagttgtatc tgattggaaa
     1501 agctcagttg gggcaccttt tatgacgatc ttcgaagcgt ctttgaattt agccgccgta
     1561 atctgagttg aattaaattt taccaccgag ccggaaacaa accctgagcc cttgattttg
     1621 attttacttg gaaccgttcc ttttgaagtt tgggcggggg aaattgacga cagaacgggc
     1681 gttccctgcc gggcgatcat cccacccgtg gcgttcagcc cagccgtctg accagtgaga
     1741 attggaaagg acaaccccaa tcccacagtg aaggcaagga atctaactat aaagaatccc
     1801 aatcctggcg cttttttcat acctacctca gaagtttgag tgcggcggct tgccgcgtaa
     1861 gaatttccac agtttttcgg cgtcaaagcg gcgtcaaacc gccgcacgtc acagtattga
     1921 tgcctgaacc tgaagtgcaa gaaaaaaatc acgggtgact gaattgatgc ggcgagtgga
     1981 ggaaaagtca gttccgggtt ccgcgcttcg gggtttttga aagggaaatg attgaacgta
     2041 cctccatcct gtagcagtta tgtccttttc gtcctttttg tcctttccat agtttaacct
     2101 gaaacccgga acccgaaacc cggaacccag cccggtaata cattgcagtc acggcggtga
     2161 gcatactatg ctccgaaacc tcatcctcat ctcacaatca gaattgaaac ggcaatggca
     2221 tttgaagaac ttccatcaac tcaaattgaa gatatctatc cgctttctcc tttgcagcag
     2281 ggtatgttgt ttcattcctt gctgacaccc ggcgcggggc tctatatcga gcaaatggcc
     2341 tgtaccctca aaggcccact tgatgtcgcc gcatttgaac gagcctggca agttctggtc
     2401 ggacgccatt cgattttgcg taccgccttt gtctgggagg gtctcgacga accattgcag
     2461 gttgtgcaat cagaggttga agtcccattt gagtttctcg actggcggaa tctgacccag
     2521 tcagatcagg aagctcaatt aaacgagtta cttacccatc aacggcacca gggatttgaa
     2581 ctcacacagg ctccgctgat gtcactgacg ctgatccagt gcggcggatt ggaattccaa
     2641 tttatctgga gtcatcatca tctgctgctt gatggctggg gagttcctat cgtattgcgc
     2701 gaggtattta ccgcctatga agccattcgg caggatcgcg cccccgccct ggctgccgtg
     2761 cgcccatatc gagactacat tggctggctc aaagaacaat ccgtcgaaca ggccgaaacg
     2821 ttctggcgac aaaacctcaa aggattttca gcgccgacgt atctgagtgc attccagaca
     2881 gcaaccgata ctgccccccg ttctgaacga tgtgaattga atctggatga acccaccagc
     2941 cagcgactgg caaccctggc ccgagaacat caaattaccc tcaacacgct gatcctcggg
     3001 gcctgggcca aactgatgag cgttcacaca accgctgacg atgtggtgtt tggggttact
     3061 gtgtcaggtc gtccggctga acttcccgga tccgatacca tgctggggtt gtttatcaat
     3121 accctgccac tccgggtggc gcttccggca cctattccgg tgaccacctg gctggccgaa
     3181 ctccagattc agcaagctga aatgcggcaa tttgaatcca caccgctggt caacattcaa
     3241 gggtggagcg agattcctcg cggaacagcc ctgtttgaca ccctgtttat tttcgaaaac
     3301 tacccatcgc cacaaagcct gaccagtggt caggaacgcc tcaccgccat ttcacttgaa
     3361 aaagttacag cccgtggcaa taccaactat ccattgacat ttgtggcggc acccggaaat
     3421 gaactccatt tcctggccgt gtacgaccaa tcagttttct cacaagcctt tgtcacaggt
     3481 ctgatggggc aaatgaccgc gctgcttgaa aacctggctc aggcacctga ccagtcgctt
     3541 ggcgagtggt ctccgttatc cgaagcccaa cgccatcaaa tcctggttga ctggaacgcc
     3601 accgccagag cctttcctga cgacacctgt attcatcacc tctttgagaa acaggttcga
     3661 caaacgccag acgcagtggc gattgttttt caggaccagt ctgctaccta tcgtgaactc
     3721 aattatcgag ccaatcagct tgccttttac ctgcaactga ttggtgtcaa accggaaacc
     3781 ctggttggtc tatgtgtgaa ccgatcagtc gaaatgctgg tcgggatgct tggtatttta
     3841 aaggccggag gcgcatttgt tccgcttgac ccgtcgtatc ccaaagaacg gctggcattt
     3901 atgcttgccg attcccaggt tccggtgctt gtgacccaat cggtcctgtt atcccgcctt
     3961 ccccggcacg acagccaggt ggtgtatctc gatcagccat tacccacaat ttcacaccgg
     4021 ccttttccag ttcaggccac ttcccacaac ctggcctatg tgatttatac ctcgggatca
     4081 actggcacgc ccaaaggcac actgcttgaa catcaagggt tgtgtaatct ggcactggct
     4141 caaatccagg acatgggctt tgctcctggt tcacgtgtgg tgcaattttc atccttgagt
     4201 tttgacgcct cagtctttga gatttttgcg acgctggtgg gcggctctac tttgtacctt
     4261 gggactcggg ttgacttgat gccgggagca gaactggctc atttcctcag acaccaccag
     4321 atcaatgtcg ccaccttacc accctcagcc gccgccgcat tgccagaggg agagtacccg
     4381 gcgctacgca ccctcatcac ggcaggtgaa ccctgtacag ccgaactggt t
//
LOCUS       DAOQDO010000195         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13419_length_4414_cov_2.620555, whole genome shotgun sequence.
ACCESSION   DAOQDO010000195 DAOQDO010000000
VERSION     DAOQDO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13419_length_4414_cov_2.620555"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1129)
                     /locus_tag="PKZ53_04350"
     CDS             complement(8..1129)
                     /locus_tag="PKZ53_04350"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNJ39698.1"
                     /translation="MNILIAPNALKGTLTALEAAQAMHRGVAHSLPEAECTIFPMADG
                     GSGTIDALLAIHGGTRIQTKVTGPLGTQVLAEFALLGDCQTAVVEMAQASGLKLVPES
                     ARDPFRTTSFGTGELLLRAVECGASTIILGIGDTATIDGGAGMLQAIGVSLTDDQGQE
                     LAAGNAPLAALSHIKLDQLDSRLRNIQVVIACDVQHQLSGPEGAVYVFGPQKGARPQD
                     LPVLEANLTRLADHLERVTGKPYRTLPGSGAAGGIGGALVSVLDARLVSGSRLLIESH
                     LFQEALSHADLVLTGEGCLDQQSLAGKGPWAVAQAAKAQQIPVFTFVGRCQIPAETAH
                     AAGFTCVIELPQPESLQHPRLTLERAVAKTIFQSSASFL"
     gene            complement(1282..3963)
                     /locus_tag="PKZ53_04355"
     CDS             complement(1282..3963)
                     /locus_tag="PKZ53_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ39699.1"
                     /translation="MKASQLLSRTLLWVLTTCAVVSIGTKAFYSTQATFSWASPGFYE
                     EYHPTEGKILVAKVIPKTPAAYVGLESGDELLTQTGAPFTGFVEYSQFRKAAQHKGVL
                     ALTVRKPDGKLTSCLIDLPTTTFEGFFSYQFGFYLILLITPLICTIIGLGVCWLRWED
                     KTARTGGLFFVCAASLFGMNSILIPGGLREFYLLYSGIAMWLSGYLCFRFFSEFPTPT
                     ALSQKYRWVRYLYLLFTLVMLSFSVVTDLAAQYSFSLARELNAYTFGSLEQIQGPVLL
                     LGFLGSPLTALPGIRGVWMADQERRFRVLWLGITVGCGPLLALFLVDLVVHHETTLRN
                     FPPWVTLIAVALYVVFPLTFGYTIVKHRVLGVRQILRRSIQYAFLSKSYWVLELILLV
                     LVLRYPVTDASQQLFMALDVSPTPMMWRGIYYCLAGFGLFWMWQVNPHLQALIDRAFF
                     RDAYNAQQVLTELSRSVRNFTDAYEVLQRVGSQINAALHVNRVAFFVHQAFLHAKPQS
                     DPIQEGMPAFVCRFEMGSTGGIDPELQLPNGTPILQKLSKDPIPLDVYFADPDSWTEE
                     LDRDPEESYIARHLEQRLFEPLNTSLIVPLATKEGVLGLMSLGPKLSEEPYTKEDKSL
                     LMAVAEATALRLENARLIALATEEAKLKRELEIARHLQQSLLPSGDPVFPDLQLASFS
                     TTAQEVGGDYYDYFVLNQSELAIAIGDVTGHGVSSGLLMALAKGGLLNQVSVDASPPA
                     VISAMNTLICITSNKRNLMTFAYAVLNVETWQLQFANAGHPFPFHYSAQSRTVRMIES
                     TAYPLGVRPQSCYETKHAELAPGDTIVFYSDGLVEARDSQGKVFGYDGLEQVIAAHSN
                     LDAHHLRDRIFDAVRSFSNGHAMDDDITLVIVKRTLA"
BASE COUNT         1106 a         1136 c         1207 g          965 t
ORIGIN      
        1 cacagattca aaggaaacta gctgaagatt ggaaaatagt ttttgcaaca gctcgttcca
       61 gggttagtct ggggtgctga agtgactccg gctgaggaag ctcgatgaca caggtaaaac
      121 cggcggcatg ggccgtttca gccgggatct ggcagcggcc aacgaaggtg aacaccggaa
      181 tctgttgagc tttggcagcc tgtgccacgg cccatggccc ttttccagcc agggattgtt
      241 gatcgaggca gccttctccg gtcaaaacca ggtcggcgtg agagagcgct tcttggaaca
      301 gatgactttc aatcagcagc cggctccctg aaacaagtct ggcatccagg actgagacca
      361 gggcacctcc gatgccgcca gcggcgccgc ttccggggag cgttcgatac ggtttgccgg
      421 tgacgcgctc caggtgatcg gccagtcggg tgagattggc ctccaaaact ggcaggtctt
      481 gagggcgagc gcctttttgt gggccaaaga cgtacaccgc cccttctgga ccagacaatt
      541 gatgctggac atcacaggca atcaccacct gaatgtttcg caatcgtgaa tccagttgat
      601 ccagcttgat atgtgataga gctgccagtg gagcattccc tgccgccaat tcctggccct
      661 ggtcgtcagt cagggaaacg ccgatggcct ggagcatccc cgcgccgccg tcaatcgtgg
      721 ccgtgtctcc gattcccaga atgatggtgc tggccccgca ctcaaccgct cgcaacagga
      781 gttctccagt tccaaaactc gtggttcgaa atggatcgcg cgctgattcc ggcaccaatt
      841 tcaaccccga agcctgggcc atttcaacca ccgccgtttg acaatctccc agaagagcaa
      901 attcagccag aacctgggtt cccagtggcc cggtgacttt tgtctggatt ctggtgccac
      961 catgaattgc cagcagggcg tcaatggtgc ccgacccacc atctgccatt ggaaatattg
     1021 tgcactcagc ttcagggaga ctgtgggcca caccacggtg cattgcctgg gccgcttcca
     1081 gggccgtgag tgtgcctttc aatgcatttg gcgcaatcaa aatattcata aagtagctgg
     1141 ggctgaagaa ggcgggctga agaagacggg ctgaagaaga caggctgaag attaacccaa
     1201 cttcttcagc cccaggcccg aagacttcag ccccaggccc gaagacttca gccctaagcc
     1261 ctgagccctg gttttttgcc cttatgccag ggttcgcttc acaatgacca gcgtgatgtc
     1321 gtcatccatg gcgtgtccgt tcgagaagct tcgcaccgcg tcaaaaatgc gatctcgcag
     1381 gtgatgggcg tcgaggttgg aatgggcagc aattacctgt tccagtccat catatccgaa
     1441 gaccttccct tgcgaatctc tggcttcaac caggccatcg ctgtaaaaca caatcgtgtc
     1501 tccgggggcc aattcggcat gtttggtttc ataacacgac tgagggcgta cgccgagcgg
     1561 gtaggctgta ctttcgatca ttctgacagt tcgactctgt gcactatagt gaaaggggaa
     1621 gggatgtccg gcattggcaa actggagttg ccaggtttcg acgttgagca ccgcataggc
     1681 aaaggtcatc agattccgtt tattactggt gatgcagatt aacgtgttca tggcagaaat
     1741 gactgccgga ggtgacgcat caactgacac ctggtttaag agcccacctt tggccagcgc
     1801 catcaacaac ccggatgaca ctccatgccc ggtgacgtcg ccaatggcaa tcgccagttc
     1861 tgattgattc aggacaaagt agtcatagta atcgccaccg acttcctggg ctgtggtgga
     1921 aaaactggcc agttgaagat ccggaaacac cggatcgcct gatggcagca aactctgttg
     1981 caggtggcgg gcaatttcaa gttcacgctt taacttggct tcttccgttg ccagtgcaat
     2041 cagccgggcg ttctcaagcc ggagcgcagt ggcttcggca actgccatca acagtgattt
     2101 gtcttctttg gtatagggtt cttcagaaag ttttggcccc agtgacatca agcccagaac
     2161 accctctttg gtggcgaggg gaacaatgag gctggtgttg agtggttcaa aaagacgttg
     2221 ctcaaggtgg cgggcaatgt agctttcctc aggatcacgg tcgagttctt cggtccagga
     2281 atcaggatcg gcaaagtaaa cgtctaaggg gatgggatcc ttgctcagtt tttggagaat
     2341 tggggtgcca tttggaagct ggagttctgg atcaattccg ccggtcgagc ccatttcaaa
     2401 gcggcagaca aaagccggca ttccttcctg aattgggtca gattgtggtt tggcgtgcag
     2461 aaatgcctga tggacaaaaa acgccactcg attgacgtga agcgcggcat taatttgtga
     2521 cccaacccgc tgcagaactt cataggcatc agtaaagttg cgaactgacc ggcttaattc
     2581 ggttaatacc tgctgcgcat tgtaggcatc acggaaaaat gcccgatcaa tcagtgcctg
     2641 aagatgaggg ttgacctgcc acatccaaaa caacccaaac ccggccagac agtaataaat
     2701 gccccgccac atcattggcg tgggtgagac gtcaagcgcc ataaaaagct gctggctggc
     2761 atcggtgact ggatatctga gcaccagcac cagcaggatc aattccagaa cccagtaact
     2821 tttggacaga aaggcgtact gaatgctgcg acgcaggatt tgtcgaaccc caagcacccg
     2881 gtgtttgaca atggtgtatc caaatgtcag tgggaagacc acgtacagag cgacggcaat
     2941 cagggtgacc caggggggaa aattccgcag tgtcgtttca tggtgcacca ccagatccac
     3001 cagaaacagc gccagcaagg ggccacatcc gactgtgatt ccaagccaga gcacccgaaa
     3061 ccgccgttcc tgatcagcca tccagacccc ccgaattcct ggaagtgccg ttaaggggga
     3121 tcctaaaaag cccagcaaca acaccggacc ctgaatttgc tccagtgagc caaaggtata
     3181 ggcattgagt tcacgggcca gggaaaacga atactgggct gccagatccg tgacgactga
     3241 aaaactgagc atgaccagcg taaacagcag atacaggtag cgaacccagc gatacttttg
     3301 ggataaggcg gtcggggttg gaaattcaga aaaaaagcga aagcacagat accctgacag
     3361 ccacatcgcg ataccgctgt agagcaggta aaattcacgt aatccgcctg ggatcaaaat
     3421 ggagttcatc ccaaagaggg aagccgcaca gacaaaaaat aatccgccgg tacgcgctgt
     3481 tttgtcttcc catcgcagcc agcaaacccc aaggccaata atggtacaaa tcaacggggt
     3541 aatcagtaga atcagataaa agccgaattg ataagaaaaa aacccctcaa acgtcgtggt
     3601 cggaagatca atgagacagg aggtcagttt accatccggt ttgcggactg ttaaggcaag
     3661 gactcctttg tgttgagccg ccttgcgaaa ctggctgtat tcgacaaaac cagtgaacgg
     3721 cgccccggtc tgagtcaata actcatcgcc tgattcaagt ccaacatagg cggcgggtgt
     3781 tttggggatc accttggcca ccaggatttt gccttcggtg gggtgatact cttcataaaa
     3841 ccctggagac gcccacgaga aagttgcctg agttgaataa aacgccttgg tgccaattga
     3901 aacaaccgca caggtggtga gcacccagag caaggtccgt gagagaagtt gggaggcttt
     3961 cataatgaca ctccgagaaa ttcgggggac ggttgctgag gtcaggtggg ggcagggtaa
     4021 gccaactcga agccaaatac aatgcagtgt gtgcgggtca atgcgagtga actggtacgc
     4081 ttgattgcgt gcctgcctga aaggtctgac gtgccagttt ctttcagaaa cagcagccgc
     4141 gtttcaatgc tcgatcagac gtgatattcc ctatccaatg cgtcaacttt cgtatctgaa
     4201 agtgttaccg ccctgtgttt ttctgagttt gaagatcaac ataacctgcg cttgaaaatt
     4261 ggtctggttt gattggttat gcactggtga ctgactccat accagatctg gaggttgagt
     4321 caggggctta ccctcagccg ggcttcaaaa ctttggaggg gctgtaaaat tcacggaaaa
     4381 atctggactt tttgacgcat ttctgacgca ttgt
//
LOCUS       DAOQDO010000196         4413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13426_length_4413_cov_2.995411, whole genome shotgun sequence.
ACCESSION   DAOQDO010000196 DAOQDO010000000
VERSION     DAOQDO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13426_length_4413_cov_2.995411"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKZ53_04360"
     CDS             complement(<1..382)
                     /locus_tag="PKZ53_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNJ39700.1"
                     /translation="MLVTYTAQATAELPIIDVLAIGAHPDDVEMNVGGTLLKLKAQGY
                     RTGILDMTRGEMGTRGTVELRAIEAHNAAQILGLDVRANLELPDGHISADDASRRKLV
                     RALRRLRSKVIFAHYPEDPHPDHAH"
     gene            complement(641..907)
                     /locus_tag="PKZ53_04365"
     CDS             complement(641..907)
                     /locus_tag="PKZ53_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39701.1"
                     /translation="MPRVKKTAPEPAPSTEKSSSKASRRGRPSHVISVPVNRQTLSIL
                     ETCVTLGQATSTSAVAAKLLARAAEDLKNSIAQQVSNNFLGKEK"
     gene            complement(1480..1743)
                     /locus_tag="PKZ53_04370"
     CDS             complement(1480..1743)
                     /locus_tag="PKZ53_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39702.1"
                     /translation="MEFHALKSEHHFNCTVKNVDEKGKETVCNGSLKEWMTAPPEVQK
                     QVGNDEALYRCRRCGAVYKGRVKRHLSSGKKLSSLPPQFPFLR"
     gene            1839..3488
                     /locus_tag="PKZ53_04375"
     CDS             1839..3488
                     /locus_tag="PKZ53_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39703.1"
                     /translation="MRIPLDPPKFTDLVQTLVRHALTPAIVFLPSRRACDDAVESFRS
                     IRSVGLSSDQIDQIEAIIDSFGDEAPFLRKHRHYSLVFRKGVAAHHAGHLPAWKHLVE
                     KLMSSGLLRAVFSTSTLAAGIDMPARSVVITASSLRSDDGHRDIKAFELAQMTGRAGR
                     RGRDKVGFAVFIPGPFQDIRLIQERLFQPPEPIESSFNANYTMVLNLLQQLSPESARA
                     LVERSFLQFQRIKQAERLRPRLERIQKLVNDDAAQCPAGNRVKTIMRYQELEHTLNRS
                     RKQVKSLRRMVDREQVAAGMDPEWTKSLIEGLEKEETTLRALLAKRDDLPCGTCSHLK
                     RCPQRAGEIAHSYEELTRLEDRMDSFEHGLWSGFEDCVKILQHFDYIDDNWRPTEQGN
                     WASFLRVDNTLFIAELLRDGFFDTPDARTMAALCGGLAAEDRDFTTSYHQPEPFLATL
                     RKAVGIAKAIEAVQDLHSFYCPMWIDKEASKLVWLWGDATRPWEELLKLTEASEGDIV
                     RLILRTADLLGQLAGLVDSHPQIAARAREATRLIRRPPIEE"
     gene            complement(3663..4376)
                     /locus_tag="PKZ53_04380"
     CDS             complement(3663..4376)
                     /locus_tag="PKZ53_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ39704.1"
                     /translation="MVLATINYLYWGSFRGSDFGQAIRNIAAKNREKLVVVVQTYTRI
                     GMLMEPSLNSALRKLKIDYTDLLLLGWWNDLPPKRILDAALALKESGKARHIMVSCHN
                     RLTFQRFIEDPNYGAIMVRYNAAHTGAETEVFPYLKAKKRPGVVAYTATRWGGLVNPN
                     NIPAGEPVPHGSDCYRFVLSNPAVNMSLIGPKNREELDEAMAALDRGPMDEDELEWMR
                     RVGRAVRSGSPQIVDKMRD"
BASE COUNT         1082 a         1069 c         1135 g         1127 t
ORIGIN      
        1 agtgggcgtg gtccgggtgg ggatcttccg gataatgggc aaagatgact ttggagcgta
       61 accggcgcag ggcgcgcacc agctttcggc gcgaagcgtc atccgctgaa atatggccat
      121 cgggtagttc gaggttggcg cgaacatcca gtcccagaat ttgcgcggcg ttgtgggctt
      181 caattgcccg gagttcaacc gtaccgcgtg tgcccatttc gcctcgggtc atatcgagga
      241 tgccagtgcg atacccctgg gctttgagtt tgagcagtgt gcctccgaca ttcatttcaa
      301 catcgtcggg atgggctccg atggccagca catcaatgat tggcagttca gcggtggcct
      361 gagcagtgta ggtaaccagc atgtgatttt acctttcgtg ttaaggtttt tttgtgccta
      421 aaagcaacca cgccaacaac ttaatcagat gttggcgtta aagtttttca caagatttgg
      481 gaagagtgct ttgggatttc agccatcagg acatctctgt atgattatag tgcctgggcc
      541 aatttacaat cattgaccct gcaagagcta tagagtaagg gaacccccaa aaatgacttt
      601 tgcgaacgac tgacaactga cgactgacca caaactggca ctatttttcc ttcccaagaa
      661 aattatttga gacttgctgg gcgattgaat ttttcagatc ttcggctgcc cgagccagga
      721 gtttcgctgc cacggcagac gttgaagttg cctgcccaag tgtgacacag gtttctaaaa
      781 tcgaaagcgt ctggcggttg acgggaactg agatgacgtg tgacggacga cctcgccggc
      841 tcgcttttga ggatgatttt tcggtgctgg gcgctggttc tggagctgtt ttttttactc
      901 gtggcattgt gaagcctcct gaatgaaaaa gaataattcc ggtttaagaa tagatcactg
      961 gcggaagatg attttggcag gaaatttcat tcattattct tgaacgaaaa tactgaagtg
     1021 aaaatgcttg aagatcggct gcataatcct ttcagtctct gaaaaaatac tttgttcaag
     1081 tgaaaaaaac aagcatatat cgctcaagaa aaagaggcct cattcaataa atcaaatcgt
     1141 gtttcacagg ctggagttaa cgcaccggta attttaagtc tgatgtaaaa tattgttatt
     1201 caacgagata tggcatggca ggtgataagt tgggaaaaga atgaaccagt ttggccagaa
     1261 gacagaaggc caaattactg ggagataaaa agatattcaa ttaagatatt gaagaaataa
     1321 tccatcaaga aataatttcc tgaagcacat ccagaactca gtggaacatc gctggtaacc
     1381 ggtcgaaaca gtgatccatt tcaccagggt tttcagaaaa gattcaaaaa ggcagcgtgc
     1441 ggaaatagat ggtttacggg aaacaaaaag atcaaatttt taccgaagaa atggaaattg
     1501 aggtggcaaa ctggacagct ttttccccga agataagtgc cgtttgaccc ggcctttata
     1561 cacagcgcca caccggcggc atcggtagag tgcctcatcg tttccgacct gtttttggac
     1621 ttctggaggg gccgtcatcc attctttcaa agacccattg cacaccgttt cttttccctt
     1681 ttcatcaacg tttttcacgg tgcaattaaa atgatgttca ctttttaagg catgaaattc
     1741 catagttgat ctcctttttc tggcaaagtg tttcaaggaa agtgaggttt tttggtacta
     1801 tccgggtcat tccacttaga tgtgttcctc cattgtaaat gcgaattcca cttgatccac
     1861 caaaatttac tgatttagtg caaacgctgg ttcgtcacgc cctgacaccg gccattgtat
     1921 tcttacccag ccgccgggca tgcgacgatg cggttgaatc atttcgatca attcgttcgg
     1981 ttggcctgtc ttctgaccag attgaccaga ttgaagccat tattgactca tttggggacg
     2041 aagcgccatt tctgcggaaa catcggcatt attcgctggt ctttcgcaag ggagtggctg
     2101 cccatcacgc gggtcatttg ccggcctgga agcatctggt tgaaaagctt atgtcctcag
     2161 ggttgctccg agcggttttt tcaacctcca cgctggccgc cggaattgat atgccggcgc
     2221 gatctgtggt gattacggcc tccagtttgc ggagcgacga cggtcaccgc gatattaaag
     2281 cctttgaact ggcccaaatg actgggcggg ctggtcgtcg ggggcgcgac aaagttgggt
     2341 ttgccgtgtt tatcccaggt ccgtttcaag acattcgcct gattcaagag cggttgttcc
     2401 agcccccgga accaattgaa agcagtttca atgccaacta cacgatggta ctcaacctgc
     2461 tccaacagct ttcccctgaa tcagctcggg ccctggtcga acggagcttt cttcagtttc
     2521 agcgcatcaa acaggctgaa cgcctccggc cacgactgga gcgtattcaa aaactggtca
     2581 atgacgatgc cgcccagtgc ccggctggaa accgggtgaa aacaattatg cggtatcagg
     2641 agctggaaca caccctcaac cgttcgcgca aacaggtcaa atcactacgg cgcatggtgg
     2701 atcgcgaaca ggttgctgct ggaatggatc cggaatggac gaaatcttta atcgagggcc
     2761 tcgaaaaaga ggaaaccacg ctgcgagcgc tcctggccaa acgcgatgat ttgccctgtg
     2821 gaacctgctc gcacctgaaa cgttgcccgc aacgggctgg tgagatcgcg catagttacg
     2881 aagaactgac ccgactcgaa gaccgcatgg atagctttga acatggcctc tggtcaggtt
     2941 ttgaagactg cgtgaaaata ctgcaacact ttgattatat tgatgataac tggcgtccaa
     3001 ctgagcaggg aaactgggcg tcgtttctgc gggttgataa cacactcttt attgccgagt
     3061 tgctccgcga tggttttttt gacactcctg atgcccggac aatggctgcg ctgtgcggag
     3121 gtttagccgc tgaagatcgt gattttacaa ccagttacca ccagccagag ccatttctgg
     3181 caaccctgag aaaagcagtt gggattgcta aggccattga agcggttcag gatctgcact
     3241 cgttttactg cccaatgtgg attgacaaag aagcgtccaa gctggtgtgg ctctggggtg
     3301 atgccacccg accctgggaa gaactcctga aactgaccga agccagcgaa ggcgacattg
     3361 tccgattgat tttgcgcacg gctgacttgc tcgggcaact tgccgggttg gttgattctc
     3421 atccacagat tgcagcccgc gcccgcgagg ccactcgact gatccggcga ccgccgattg
     3481 aggaataaag ctgaagacag caggctgaag aaaacgggct gaacaatcct cgggctcagg
     3541 gcttggggct gaagactcgc aagctcgggg ctgaagaaaa cggatttcat gccctgagcc
     3601 caacttcttc agtccgactt cttcagcccg ttttcttcgg cccgatgttt ttagcccgcc
     3661 gttcaatccc tcatcttatc aacaatttgc ggtgatccgc tacgcacggc ccggccaacg
     3721 cgtcgcatcc attccagttc gtcttcatcc attggtccac gatccagagc tgccattgct
     3781 tcgtcgagtt cctcccggtt tttgggaccg atcaaactca tattcaccgc cgggtttgaa
     3841 agtacaaatc ggtaacagtc tgacccgtgc ggaacaggct cgcccgcagg aatattgttg
     3901 ggattgacga gtcctcccca ccgagttgcg gtgtaggcca caactccagg tcgttttttg
     3961 gccttgagat agggaaaaac ttcggtttct gcgccggtgt gggccgcatt gtaccgaacc
     4021 atgattgcgc catagtttgg atcttcgata aatcgctgaa atgtcaggcg attgtggcac
     4081 gaaaccataa tgtggcgggc ttttccggat tctttgagtg ccagtgccgc atccagaatc
     4141 cgtttgggtg gaaggtcgtt ccaccatcca agcaggagca gatccgtgta gtcaatcttc
     4201 agtttccgca aagctgaatt gagtgacggc tccatcaaca ttccaattcg ggtataggtc
     4261 tggaccacga cgaccagttt ttcacggttt ttggcagcaa tgttcctgat cgcctgacca
     4321 aagtcgctgc ctcggaatga tccccagtac aggtagttga tagttgctaa aaccataacc
     4381 aaatagtgca ttagctataa ttcttcgcgt taa
//
LOCUS       DAOQDO010000197         4406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13468_length_4406_cov_3.595725, whole genome shotgun sequence.
ACCESSION   DAOQDO010000197 DAOQDO010000000
VERSION     DAOQDO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_4406_cov_3.595725"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKZ53_04385"
     CDS             <1..370
                     /locus_tag="PKZ53_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNJ39705.1"
                     /translation="YHDKDGICWPVSIAPFEVVVTPVNYADEEISRVANQVYDGLKAQ
                     GIDVLLDRRDERPGVKFKDADLIGIPYRVVIGAKKLKDQKVEFFTRSTRSSEDIAVDD
                     IINHVAGAVRAEHERLKKAE"
     gene            973..1740
                     /locus_tag="PKZ53_04390"
     CDS             973..1740
                     /locus_tag="PKZ53_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ39706.1"
                     /translation="MYHYHHLTIVIPVHNEAELLPSVVASLPDFVDHVVFVDDASTDD
                     TQVVLRHLAESQPGRFTVLRHLQNQGVGAATITGYRHALANKADLIVLMDGDGQMDSR
                     DLPVMLDTLIGGPFDFVKGNRFAHPSINHMPWTRYLGNYILSWLTRLALGYKQPVDAQ
                     CGFTVFRATGLRQLDLNQLFPRYGFPNDLLFEVAAKGLYFTSVPVRTIYGNEISGINP
                     FVVLPTILGLIARGYWRRLGRQSSKPRAQWSETRASQ"
     assembly_gap    1885..1984
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2209..3426
                     /locus_tag="PKZ53_04395"
     CDS             2209..3426
                     /locus_tag="PKZ53_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ39707.1"
                     /translation="MIQPAARTRNYNYAIRNIVVEAKKVEASGRPVTYLNIGDPVIYG
                     FQPPQSLMEAMAKAVRDGNNGYAPSNGTVEAREAIAEDSAKFGIPISPEDVIVTSGAS
                     EGADLILSALLEAGDDVLTPCPTYPLYSAITSKLGAKENYYHLDPKNNWLPNLDEIRS
                     LITPRTRAMVVINPNNPTGTVYSKELLQGLLDIAAEHNLVVLADDVYHRMTYGPAGAR
                     LAELAQPMEVPVITLESLSKSHLVPGWRVGWMTFTNKPAMADLIPAIRKMADARLCSP
                     LPTQCVIPAALSDLSHLKDTMDAMKLRADITVESINAIPGMNCTRPEAAFYIMAQMEN
                     LAGATDEEFVLALLRETGILFVYGSGFGLNPQDGYFRIVFLPQPEVLRDVYTRLGEFA
                     TAWPNRARQAAQG"
     gene            complement(3677..4237)
                     /locus_tag="PKZ53_04400"
     CDS             complement(3677..4237)
                     /locus_tag="PKZ53_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39708.1"
                     /translation="MVQRLFLFLGCLGLILIGGTDCSILGQAPANKQAAQMAAMKKLE
                     GMVGDWAGSGWIEMGPQRQTFTSLETVQSRLNGMVLIIEGLHTSKLEAGGPEKVIHHA
                     LAWLSFDDQTNQYRMRPHKADGASLDTFLVPTENGSWQWGIPSPNGGSIRYTITLKEK
                     KWQEVGEFSMDGKQWRKFFEMNLEKK"
BASE COUNT         1020 a         1115 c         1046 g         1125 t
ORIGIN      
        1 ctatcatgat aaggacggga tttgctggcc agtctccatt gccccctttg aagtcgtggt
       61 cactccggtc aactacgccg acgaagaaat ctcccgtgtc gccaaccagg tgtatgacgg
      121 cttgaaagca cagggcattg acgtgctgct tgaccgccgt gacgaacgcc ccggtgttaa
      181 atttaaagat gccgatctga ttgggattcc ctatcgcgtt gtgattggag cgaaaaagct
      241 gaaagaccag aaggttgaat tctttacccg ctcgactcgc tcatccgaag acatcgccgt
      301 ggacgacatt atcaaccacg ttgcaggtgc ggtgcgtgct gaacacgaac ggctgaagaa
      361 agcagaatga agaagtcagt accacctgcg tcagcgggtg ggataagaat gaatgaagaa
      421 tgaagaatga agaatgaaga atgaagaggt tttagccgct gactttcttc attctcaatt
      481 ctcaattctt cattcattcc gatgcgccca gcaggactcg aacctgcgac ctacggcttc
      541 ataccactat agctttcgct acccccgttg ggtttgtggt ctggactgta tcttcaccat
      601 ttcaggtgca acacgtgcag tctctacgga acccctggag tttacttttt gaatttctgc
      661 aactccaaaa gcatttaccc tggacaactc aaccgatttc aggtggagtg ccagccttca
      721 atgcctcaaa caaaggtttc ctcggtatta ccagcgatga gtactcatcg ggaaggcttc
      781 accgatatag tgctgtgcat tgcggcaatt ttgtttcttg ccgcaagtcc aattttcggt
      841 tagaaggccg ttgctctatc caactgagct atgagcgcgc aaagaacaat ttgaacggag
      901 cgcgactata gcaaaacctg agctggcgtt tccagacgtt ttttctccag cgacgctcat
      961 catcaacgtt caatgtacca ctatcatcat ctcaccatcg ttattccggt tcacaacgaa
     1021 gcggaactcc ttccctcagt tgttgcttcc ctccctgatt ttgtagatca cgtggttttt
     1081 gtggatgatg ccagcactga tgacacgcag gttgtcctcc gacaccttgc agaatcccaa
     1141 ccagggcgat ttacagttct ccgacacctc caaaaccagg gtgtcggggc agccaccatc
     1201 accgggtatc gtcatgcctt agccaacaaa gctgatttaa tcgtgctcat ggatggtgat
     1261 ggtcagatgg atagtcggga cttgccagtt atgctcgaca ccttgattgg cggtccattt
     1321 gattttgtga aaggcaatcg cttcgcccat ccgagcatca accacatgcc ctggacccgt
     1381 tatcttggga actacatctt gagttggctg acccgactgg cactcggtta caaacagcca
     1441 gtggatgctc aatgtggctt tacggtattc cgagccactg gattacgcca actcgatctc
     1501 aatcaactct ttccaagata tggcttcccc aacgatctgt tgtttgaagt cgccgccaaa
     1561 gggctgtact tcaccagcgt cccggtgcgg accatttatg gcaatgaaat ttccggaatt
     1621 aatccatttg tggtgctgcc aaccattttg gggctgattg cccgtggtta ctggcgtcgg
     1681 ctaggtcggc aaagcagcaa gccacgcgcc cagtggagtg agacgcgagc gagtcagtag
     1741 agcaaccagg aaactggtgc ttggtgactg gtgcctggtg cttcgttctt ggttcttcgt
     1801 tcttggttct tggttcttcg ttcttggttc ttcgttcttg gtgcttcgtt cttggtgctt
     1861 cgttcttggt gcttcgttct tggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnncaccag gtacgaagca ccaagtacga aattgcaaat tcccctcaaa aaaggtaaaa
     2041 ctacgactgt tgccattcct gtaattccct cattcataac aggattttcc cttgcattgc
     2101 actcgtcaaa caccaatacc agtcattttt tctgatgacc gttttctgac ggcacccgat
     2161 tgaccattga tgtaacccgg aacccaccat ttcaccgagg atctgagtat gattcaacca
     2221 gcagcacgca cccgaaatta caactatgcc atccgcaata ttgtcgtcga agccaaaaaa
     2281 gtcgaagctt caggccgccc agtcacctat cttaacattg gtgatccagt gatttatggg
     2341 tttcaaccac cccagtcatt gatggaagct atggccaaag ccgtccgtga cggcaacaac
     2401 ggctacgctc catcaaacgg aacggttgaa gcccgcgaag ccattgccga agattcagcg
     2461 aaatttggaa tcccgatttc acctgaggat gtgattgtga cctctggggc ttctgaaggg
     2521 gctgatttga ttttgagtgc cttgcttgaa gcaggtgatg acgtcctgac cccctgcccg
     2581 acctatccgc tgtattcggc cattacttcc aaactagggg caaaggaaaa ctactatcac
     2641 ctcgacccga aaaataactg gctaccgaac ctggatgaaa ttcgcagttt gattacccca
     2701 cgcacccgcg cgatggtggt tatcaatccc aataatccaa cgggaacagt ctattcgaag
     2761 gaactcctcc agggcctgct tgatattgcc gctgaacaca acctggtggt gctggccgat
     2821 gacgtctatc accggatgac ctatggtccg gcgggtgccc ggctggcaga actggctcaa
     2881 ccgatggaag ttccagtcat cacgctcgaa agcctttcaa aatcgcacct ggttcccggc
     2941 tggcgcgttg gctggatgac ctttaccaac aaacccgcga tggcggacct gattccagcg
     3001 atccggaaaa tggcggatgc ccgtctgtgc agcccgctgc caacccagtg tgtcattcca
     3061 gcggcacttt ccgatctgtc tcatctgaaa gacacgatgg atgcgatgaa attgcgtgct
     3121 gatatcacgg ttgaatccat caatgcgatt cctgggatga actgcacccg tccagaagcg
     3181 gccttttata ttatggccca aatggagaac ctggccggtg ccacggatga agagtttgtg
     3241 ctggccttgc tgcgcgaaac cggaattctg tttgtctatg gttccgggtt tggtcttaac
     3301 ccgcaggatg gctatttccg cattgtattt ttgccgcaac ccgaagtttt gcgcgatgtc
     3361 tatacccgat tgggtgaatt tgccaccgcc tggcccaatc gcgcccggca ggcagcccag
     3421 gggtagaagt gagcgaagaa tcgaactgga agcgaggagt gagtggctgt aataagcacc
     3481 ttgtcataag tttcctgaga tattggattt ggtaagtgct tgattttttt ccgtgttttc
     3541 cgtgttttcc gtgttggaaa tgtctggaaa ttttcggcaa ggtacttagc aaagtgcttc
     3601 agctttgatt tccgactttg gtctgtcaac ccataaattc tgtcgttccg actacttcgc
     3661 tatttcgctc gcattgctat ttcttctcca aattcatctc aaagaatttg cgccactgtt
     3721 tcccatccat tgagaactcg ccgacttcct gccacttctt ctctttcaat gtaatggtat
     3781 agcggatgct cccgccgttg gggctgggga tcccccactg ccagcttcca ttctcggtgg
     3841 gaaccaaaaa ggtgtctaac gaagcgccat cggctttgtg cgggcgcata cggtactgat
     3901 tggtctggtc atcaaatgac agccaggcca gcgcatgatg aatgactttt tcaggcccgc
     3961 cggcttcgag tttggatgtg tgcagccctt cgataatcaa aaccatgcca tttaaccggg
     4021 attgaaccgt ttcaaggctg gtaaaggttt ggcgttgggg gcccatttca atccatccgc
     4081 ttccagccca gtctccgacc atgccttcca gctttttcat agctgccatc tgggcagcct
     4141 gtttattggc aggcgcttgc cctaaaatcg agcaatctgt tccaccaatc aaaatcaaac
     4201 cgaggcatcc taaaaaaaga aaaagtcgct gaaccatcgt gagtttcctc caaattgtga
     4261 atgggtacat ttcagtcaca gtaaaaggag tggctcacag ggccaagcga cgatttctgg
     4321 atggttgatt ttgctaactg aacgggaaaa aagcagaatg aagaatgaag aatgaagaat
     4381 tgagaatgaa gaattgagaa tgaaga
//
LOCUS       DAOQDO010000198         4405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13472_length_4405_cov_3.122989, whole genome shotgun sequence.
ACCESSION   DAOQDO010000198 DAOQDO010000000
VERSION     DAOQDO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13472_length_4405_cov_3.122989"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKZ53_04405"
     CDS             complement(<1..265)
                     /locus_tag="PKZ53_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNJ39709.1"
                     /translation="MTQKIFEAAEMIKSRRDVLKAGLVAGITGAAAIIGDSLLFQPEV
                     QAQGKGPAKGGAASSKANDIVIMNVALALEHQAIAAYAIGADTG"
     gene            complement(283..1050)
                     /locus_tag="PKZ53_04410"
     CDS             complement(283..1050)
                     /locus_tag="PKZ53_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39710.1"
                     /translation="MNKNTIGVLLIGLFIGFGLGVTFTKSSNPTPPAPPAESTATAPK
                     SAPPLSPTAGQQLPANHPAVEGEIAPPKPAGAAPATNQELETATIEANTNLGDYILQM
                     KAAAACYQANQFDQALVFLTRAQVLRPDSVDVLLGLGTTNAALEKYDEAEKYLTKALK
                     VTPNDPEANAELAVTYNKQKKYDVAIAAANRSLKAAPGQERALEALVEAYLEKKNTRE
                     ASTALAKLSGLNPSNPSLAVFRQQLEKLQGQGPLPSH"
     gene            complement(1231..1791)
                     /locus_tag="PKZ53_04415"
     CDS             complement(1231..1791)
                     /locus_tag="PKZ53_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ39711.1"
                     /translation="MKLAVTLVFLLGVSLVSGSTYLNSKLFAHASGSVARVAVTSVEP
                     AAPQDKKMQSRTMPGKMVLDDEDAGVTMYAGDKGKVDFDHDQHAALDTCVTCHHTNTN
                     KLTKAVEEDVMKCAVCHKDEETECATEGTNEDRKFKGKTAISSEDAFHGTGSPDRLAG
                     CIDCHKARKDKPKAQAFTKCAECHKK"
     gene            complement(1926..3125)
                     /locus_tag="PKZ53_04420"
     CDS             complement(1926..3125)
                     /locus_tag="PKZ53_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ39712.1"
                     /translation="MSSSKTRMQPNPEPGTRNPEPAVKDRLKAGVFLFFSMAALAIVV
                     TACSKRPEIPKETGKAPAPPSIGISPGVPAIIPVTLKQGTDISKFTHKSHERRPKPDG
                     TPDPAGEMVSCESCHQREKNITAPEFPGHSACLTCHLKEFNSVDASSIGMCTICHQNL
                     DTIDAPLVSFPARKSYNTFFDVQQHQNHVNYSFPDKPNEKVACAFCHPSQDKGVKVGF
                     PGYPERKFDGALVTLVKNGVGVAFPAHPECYACHTEQGGKVTPGAHTVQPGALGPGNC
                     AACHPTLIDPKANPPQPLPPGAQLLLTKISSPAYRYKFSHRDHEVKGMKCIECHNINS
                     SYNTSEIPQPKAKAHLAKKSVAGQGCFSCHNGKRAFGDEDFNSCERCHSKQDLAGSSQ
                     QAMAFNR"
     gene            complement(3225..>4405)
                     /locus_tag="PKZ53_04425"
     CDS             complement(3225..>4405)
                     /locus_tag="PKZ53_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39713.1"
                     /translation="IPTKPVAKGPVDYSKFNHADHDGDVRKLTSSGTMKLDCTYCHLV
                     DDSDKLQAGFGDFPCETPDGAKALAKTRMVKHSACIDCHKPQFVGAGVKQAMCLICHT
                     KSGLQRGLPVRNFPNEAGQSEFDVEFPHSKHKKYVKFVLNQSPSTVQVASLVGLPSGS
                     SNPIRTTVATVAADSNFACAACHSVETAGVFAARPEHRSCFVCHSEKVEKRYEDAQSE
                     PYATNCAGCHPKMQAKPYAAAHQIQFQIASAKTEAGALRFRHDSDHAILQQIPGTQEI
                     LTCVHCHGEISDAVARVKFDADFPKLKARDEKKAPDKQLYKDITEYSDFLVSKYPQSC
                     GGCHSLKKKPLIVGWLSGSNKFGNIFSVSDCDRCHAQDIIDKDINYHKKQLNPPKKTE
                     "
BASE COUNT          983 a         1042 c         1166 g         1214 t
ORIGIN      
        1 gtcccgtatc agccccgatg gcataggcag caatcgcctg atgttcaagc gccagggcaa
       61 cattcataat cacaatatca ttggctttgc ttgaggccgc gccacccttc gctggtccct
      121 ttccttgagc ctgaacctct ggctggaaga gtaacgagtc tccaattatc gcagccgcac
      181 cagtgatgcc ggccaccaac ccggctttga gaacgtcacg tcgtgatttg atcatttccg
      241 cagcttcaaa aattttttgc gtcatacctt ccctctcaaa gttcaatgtg aaggcaaggg
      301 cccctgcccc tgcagttttt caagttgttg gcgaaagaca gccagactgg ggttgctggg
      361 attgagtcct gaaagtttgg ccagcgcagt actggcctca cgggtgtttt tcttttccaa
      421 ataggcttca accagggctt caagggctcg ttcctgccct ggggcggctt tgagcgaacg
      481 attggcagcc gctatcgcaa catcgtattt cttttgttta ttataggtta ccgcaagttc
      541 tgcatttgct tccgggtcat tcggagtcac ttttaaggct ttggtcagat atttttcggc
      601 ttcatcatac ttctcaagtg cggcattggt tgtccccaat cctaacaaaa catccacact
      661 atcgggccgc aggacctggg cacgggttaa aaagaccagc gcttggtcaa attgattggc
      721 ttgatagcac gccgctgcgg ccttcatctg aagaatataa tcgccaagat tggtgttggc
      781 ttcaattgtc gccgtttcca gctcctgatt ggtggcaggg gcagcaccgg ctggttttgg
      841 cggtgcaatt tcaccttcca cagccgggtg attggctgga agctgttgac ccgcagttgg
      901 tgagagtggt ggtgctgatt ttggggcggt tgcggttgac tcggctggtg gagccggagg
      961 ggtcggattt gatgatttgg tgaacgtgac ccccaaacca aacccgataa ataatccaat
     1021 caataaaacg ccgatggtgt ttttgttcac gagagcctgc aaacaaggaa ggcagaaagc
     1081 ataggatttt ccaagctttc tgccttgtgg agagggttaa atgatgaata ccatttcgta
     1141 accgccattc tgcattcatc aatagccagg tgatgaaaga cttccattcc ctgaccactg
     1201 acggctgatg acaaactggc tttaaaatac ctatttctta tggcattcgg cacacttggt
     1261 gaaagcctgg gctttgggtt tgtctttccg ggctttgtgg caatcaatac aacctgccag
     1321 ccggtcaggg gaacccgttc catgaaatgc gtcttcagat gaaatcgcgg ttttgccttt
     1381 gaatttgcga tcttcgttgg tgccttcagt ggcgcattcg gtttcttcgt ctttatgaca
     1441 gaccgcgcac ttcatcacat cttcttcaac ggctttggta agcttattgg tgttggtgtg
     1501 atggcaggta acgcaggtat caagcgccgc atgctgatcg tggtcaaaat caactttgcc
     1561 tttatcaccg gcatacatgg tgacgccggc atcttcgtca tctaaaacca ttttgccggg
     1621 catcgtccgg ctctgcattt ttttatcttg tggtgctgct ggttcaaccg aggttaccgc
     1681 gaccctggca actgatcccg aagcatgggc aaagagcttc gagttcagat aggtactgcc
     1741 ggaaaccagg gaaaccccta acagaaacac aagggtaact gcaagtttca taacttgttc
     1801 ctttccttca ggtggttcat cagcttttat tgaataaata tcgtgggaac agtgtgatgg
     1861 gcagggtcaa caatacgttg tatttgccgc aaatgcaaga ttttgagttg ctttcaccac
     1921 cgtaactacc gattgaacgc cattgcctgc tgagaacttc cggctaaatc ctgttttgag
     1981 tggcaccgtt cacaactgtt gaaatcctcg tctccaaacg cccgttttcc attatgacag
     2041 ctaaaacacc cttgcccggc aactgatttt ttcgcaaggt gggctttcgc cttcggttga
     2101 ggaatttctg aagtgttgta actgctgttg atattatgac actcgataca tttcatgcct
     2161 ttgacttcgt ggtcacggtg cgaaaatttg taccggtatg ctggactgga aattttggtg
     2221 agcagcaact gggcacctgg aggcagaggc tgtggtggat tggctttcgg gtcaatcaac
     2281 gttgggtgac aggcagcgca attgcccggg ccaagggcac caggttgaac ggtgtgagca
     2341 cccggtgtca ctttgccacc ctgttcggta tggcaggcat aacactctgg gtgagccggg
     2401 aaggcaacac caactccgtt cttgaccaaa gtgacaagcg cgccatcaaa tttgcgctcg
     2461 ggataccctg gaaatccaac tttgacaccc ttgtcctggc ttggatggca gaaggcacag
     2521 gctacttttt catttggttt gtccgggaac gagtaattca catgattttg atgttgctgt
     2581 acatcaaaga acgtgttgta gcttttgcgc gcgggaaagg aaaccagtgg ggcgtcaatc
     2641 gtgtccaggt tttggtggca gatggtacac atcccaatgg aactggcatc aacggaattg
     2701 aattctttca aatggcaggt cagacaggca ctatggccag gaaactcagg agcggtgata
     2761 tttttctcgc gctgatggca cgattcacac gaaaccattt cacctgctgg gtcaggggta
     2821 ccatctggtt ttggtcgtcg ctcatggctt ttgtgagtaa attttgaaat atcggtcccc
     2881 tgtttgaggg tcacggggat aatggctgga acaccaggag aaatgccaat ggacggcggg
     2941 gccggagctt tcccggtttc tttcgggatt tccgggcgtt tactacacgc cgtcaccact
     3001 atcgccagcg ctgccatcga gaagaacagg aacacaccgg ctttgagccg atccttgaca
     3061 gcgggttccg ggttccgggt tccgggttcc gggtttggtt gcatgcgagt tttagaagat
     3121 gacatggggt ttaacctgga attcggccac atatagatgg atcccgttcc tgttgtttct
     3181 ggttcagccg cgttatgtgg gaaggcaaca atttttcccg gcgcttattc agttttcttg
     3241 ggaggattga gttgtttttt gtgatagttg atgtctttgt caatgatatc ctgggcgtgg
     3301 catcggtcac agtcactgac actgaaaata ttgccaaatt tgtttgatcc tgaaagccag
     3361 ccaacgatta acggcttttt ctttaaggaa tggcatccgc cacacgattg agggtatttt
     3421 gaaaccagga agtctgaata ttcagtgatg tctttataga gttgcttgtc tggggctttc
     3481 ttttcatccc gagcttttaa ctttggaaaa tcggcatcaa atttcacccg ggcaacggca
     3541 tcactgattt cgccgtgaca gtgcacacag gtgaggattt cttgagtacc aggaatttgc
     3601 tgaaggatgg cgtggtcaga atcgtgtcgg aaccgcaacg ctccagcttc agttttggcg
     3661 ctggcaatct ggaactggat ctggtgggcc gccgcatagg gtttggcctg cattttggga
     3721 tgacacccgg cacagtttgt ggcatagggc tcactttggg cgtcttcata tcgtttctcg
     3781 accttttcac tatggcagac aaaacaggag cggtgttcgg gtcgcgcagc aaaaactccg
     3841 gcggtttcaa cactgtggca ggccgcacag gcaaaattag aatcagccgc cacggttgcc
     3901 acggtggttc gaattggatt tgaactacca gacggcagac caaccagaga cgcaacctgg
     3961 acggtggatg gtgactggtt caatacaaat ttgacgtact ttttgtgctt gctatgtgga
     4021 aattcaacgt caaattcaga ttgcccggct tcgttgggga aattccgaac cggcaatcca
     4081 cgttgaagtc cagatttggt gtggcagatc aaacacatgg cctgtttgac gcccgcccca
     4141 acaaattgag gtttgtggca gtcaatacag gcactgtgtt tgaccattcg agttttggcc
     4201 agggctttgg caccatctgg tgtttcacag gggaaatcgc caaacccggc ttgtaatttg
     4261 tcggaatcat ccaccagatg gcagtaagtg caatccagtt tcatcgttcc ggaactggtt
     4321 aatttgcgaa cgtcgccatc gtgatcggca tgattaaact tactgtaatc aaccggccct
     4381 ttggcaactg gtttggtggg tatgg
//
LOCUS       DAOQDO010000199         4397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13514_length_4397_cov_4.831644, whole genome shotgun sequence.
ACCESSION   DAOQDO010000199 DAOQDO010000000
VERSION     DAOQDO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13514_length_4397_cov_4.831644"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            624..866
                     /locus_tag="PKZ53_04430"
     CDS             624..866
                     /locus_tag="PKZ53_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39714.1"
                     /translation="MDLKAADYLRHIFEHKIKIHSPWNPEKRQALASLFDLDEVEPIA
                     NEYEARQRRHLPQWNSSETSDDKWVDAELFQVEPEE"
     gene            1120..2514
                     /locus_tag="PKZ53_04435"
     CDS             1120..2514
                     /locus_tag="PKZ53_04435"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HNJ39715.1"
                     /translation="MDLLLGTIGSVKMPNILNFPNWEILSIEETAHNRLVRAQPLDEP
                     MCHTCEGTTLYRFGTKDQLYLDLSTLNKRTGIQINRQRFKCRDCKTVFWQPLDVMDET
                     RRMTKRLVEYLKQASLKKTFASLAEEIGVTEGLVRQIFKDHVAQLEKQYTPQFPKWLG
                     MDEIYLLKKSRCVFTNLEQSTIIEFLKSRDKDSVSVFLSKVKVAERRKVEIVAIDMWR
                     PYRDLATVYFPQATVVIDKFHVVRMANDGLDEVRKTIKADLTPVQRRSLKRDRYILLK
                     RNSDLNMTDLLFLDTWLKNFPDLALAYRLKEEFYEIYTAQSKEAAIKRYRKWKDQIPT
                     DPKHVITQAYKPLVTAVENWQTEIFNYFDHPVTNSVTERLNGLAREVFRLGRGYSVEV
                     LRAKLLFSEGVRVKKPGRFPRNWPVPTVGKDLPGDVLYNYLGLGSQVMPQPPELDFGA
                     DISTLIEKLQQGEI"
     gene            2660..3592
                     /locus_tag="PKZ53_04440"
     CDS             2660..3592
                     /locus_tag="PKZ53_04440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ39716.1"
                     /translation="MHEVVQAASGSNVFVYALTSGVMFGLTMLEIAAFRHWFFLRQSI
                     RPIQAVEGYSIWEGFPESIRQKCLEALSDLSRSLDIPTPTLRPTLDVGMGVGSHIFSK
                     RPELHFGLFLLVALPPPMLKAMLYHEFGHIKLGHVPILTMIGKLMVWCDVYPEDDLKY
                     SSYWWFYSRFVEPLVSKSKDTATELRRSYEHEADVFAAKICGPRQYLKALKRNTALRF
                     SGPLGNRLLLERLSRLDNDGRVLNTSDRLLFWYRMGLSKTKSPNQYDRFYPSQALREQ
                     RVWAEFELSEAHFPNLKQERVAGANKIFNSDRKI"
     gene            complement(4057..>4397)
                     /locus_tag="PKZ53_04445"
     CDS             complement(4057..>4397)
                     /locus_tag="PKZ53_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ39717.1"
                     /translation="GSFTITVTATDNCGATSTRTFVLNVNAYGKGPIIASFTPPSGPF
                     GTSVTISGFNLGTVRQVIFNNQPATFTIDSNTQITATVPFGATTGPISVVSTTGTATS
                     ATPFTVIRTK"
BASE COUNT         1136 a         1058 c         1109 g         1094 t
ORIGIN      
        1 atcaccgggc gcaacgccag cagcccccga cgatggaggg tcgggtttcg attgacgagt
       61 acaagttccc ggctcctagc cggcaggccg gcaaatagta cctccgtgat ttgttttgga
      121 atccggattt gatcaagttt caaatcgggt ccaggcacaa ttaccgctcg ggctgatcgg
      181 gtcagacggc ggcccagcac ctcacggcga agaaaagctc caacaccagt cttttcgtca
      241 aatgcgcaat ggaagcattt ttcaatgggt cttgatacat ccttcccctg tttcaggttg
      301 gacagcagcc aacaatacgc tttggtcaat ggatgtgggc caaattcact ggctggccgt
      361 aaccagggag gaagaacata caagtggcta agtgtggatg aatatccttc caatttgacc
      421 tcaaccggct ccttaaaatg aatgatgccc aacccccctt tgtctgcaaa ctctttcgca
      481 ttcttcaata gatcttccgc gaccctaaag tcagtcagtg tttcagggtt ctccgtagaa
      541 gccagttgaa aaattcaagt cgccagaggt aatctgaaac cgtattatcg agtagaaaac
      601 ggaaacacgc agaggtttaa gcgatggacc tgaaagcagc agattacctt cggcatatat
      661 tcgagcataa aatcaaaatc cattcgccct ggaaccctga aaaacgccag gcgctcgcaa
      721 gcttgtttga cttggatgaa gtggagccaa ttgccaacga atatgaagct cgccagcgaa
      781 ggcacttgcc ccaatggaat tcatccgaaa ccagtgatga taagtgggtg gacgcagaat
      841 tatttcaggt tgaaccggaa gaataacaat tcggaaaagt gaaaaatatg aaagcccctg
      901 aacaaacaaa tcagcctaaa aatatcctca agttcaaagc tcgtaccaca actatggttc
      961 ccaaccaaga tacggttgga attcggtggg aggcgcgttt acttgctggc cttctgtgca
     1021 gtttatgggg ttcagttttg gcccatctca tttgcaaaaa cctcttttct gtaagtccag
     1081 tcccagttct ggcggcatct tttatactca gctttgtgtg tggatttatt attggggacg
     1141 attggttcgg ttaaaatgcc caacattctg aacttcccga actgggaaat cctctccatt
     1201 gaagaaacgg ctcacaaccg cctcgtccgg gcgcagccat tggacgaacc aatgtgccat
     1261 acctgtgagg gtacaacact ctatcggttt ggcaccaaag accagcttta cctggacctg
     1321 agcacgctca acaaacgcac cggcattcag atcaatcggc aaaggttcaa gtgccgagac
     1381 tgcaaaacag ttttttggca accattggac gtgatggatg agaccaggcg gatgaccaag
     1441 cgcctggtgg aatatctcaa acaagcctcc ttgaaaaaga cgttcgcaag tctggccgaa
     1501 gaaatcggag tgactgaggg gctggtccgc caaatcttca aggaccacgt ggcccaactt
     1561 gaaaaacagt acacacccca gtttcccaag tggctgggaa tggacgaaat atatttgctc
     1621 aaaaagtcgc gctgcgtttt caccaacctg gagcaaagca ccattatcga attcctcaaa
     1681 tcacgggata aagactcggt ttccgtcttc ctttcaaaag tcaaagtggc cgagcggcgg
     1741 aaagttgaga ttgtcgccat cgatatgtgg cggccttatc gggatctggc gacggtctat
     1801 ttcccacaag ccacggttgt gattgacaaa ttccatgtgg tccggatggc gaatgatggg
     1861 ttggatgagg ttcggaaaac gatcaaagcc gaccttactc cagtccagcg gcgatcactg
     1921 aaacgggacc ggtatatttt gctcaaacga aattctgatt tgaacatgac tgatttgctt
     1981 ttcctggaca cctggctcaa gaattttccc gatctggcct tggcctatcg cctcaaggag
     2041 gagttttacg aaatctatac agctcaatcc aaggaagctg ccatcaagcg gtaccgaaaa
     2101 tggaaagacc aaattcccac cgatccaaag catgtgatta ctcaggcgta taagccgttg
     2161 gtgacggcag tggaaaactg gcaaaccgag attttcaatt acttcgacca tccggtgacc
     2221 aactcggtaa cggaacgact caatggcttg gcgagagaag tttttcggct gggacgtggc
     2281 tattccgtgg aggtgctgcg ggcgaaatta cttttttccg aaggcgtgcg ggtcaaaaag
     2341 ccgggacgtt tcccccgtaa ctggccggtc ccgaccgtcg gaaaagattt gccgggagac
     2401 gtcttataca attatcttgg tcttggttcg caggttatgc ctcaaccacc ggaacttgat
     2461 tttggagccg atatttcaac tctgattgaa aaactgcaac agggcgaaat ttgaaaagga
     2521 aaaaatgagg cacttcacaa aaacaatgct tgtttgccta tttcaacccc ctattcccag
     2581 gctgatcggg gttctcattc tatgctttgc gtggtggttg atggtaatcg gtgtctatgg
     2641 aggtctcgca gtttattttt tgcatgaggt tgtccaagcc gcttccggtt ccaatgtatt
     2701 cgtgtacgcc ctgacatcgg gggtgatgtt tggtctgaca atgctggaaa tcgctgcttt
     2761 ccgacattgg tttttcctgc gccaaagtat ccgccccatt caagctgtgg aaggatactc
     2821 aatctgggaa gggtttccag agtcaattcg tcagaaatgt ttggaggccc tttccgatct
     2881 ttcaaggtcc ttggacattc ccacccccac cttgcgcccg acgttggatg ttggaatggg
     2941 tgtagggtcc cacatctttt caaagcgtcc agaattgcat tttgggcttt tcctgctggt
     3001 tgccttgccg cctccgatgt taaaggcaat gctttaccat gagttcggtc atatcaaact
     3061 gggacatgtt ccaattctaa ccatgattgg aaaattgatg gtgtggtgtg acgtgtaccc
     3121 tgaagatgat ctgaaatatt cttcttattg gtggttttac tcccggtttg tcgaaccact
     3181 ggtatccaaa agcaaagaca cagccaccga gttacgaaga agttatgaac acgaagctga
     3241 tgtgttcgcc gcaaaaatct gtggcccacg acaatacttg aaggcgttaa aacgaaatac
     3301 agctcttagg ttttcggggc ctttggggaa tcggctgctt ttggagcgat tgtctcggct
     3361 ggataacgat ggcagggtac taaacacttc ggaccgcctc ttgttttggt atcgaatggg
     3421 tttatccaaa accaaaagtc caaatcaata tgaccgcttt tatccatccc aggccctcag
     3481 agaacaacga gtttgggctg aatttgaact ttcagaagcg cattttccca atttgaaaca
     3541 agaacgagtt gccggcgcga acaagatttt caactctgac cggaaaattt gaacaccctt
     3601 aaaatcagag cttttcaacc ggcttcttcg gagaaccgga ttttcaaacg ggtaggggac
     3661 gccggcagcc gtggcggcag ccttaatccg ggtttcggct gccgctgcgt catagcgatt
     3721 ggggggacgt tcggcctgca taggtgaaac agaaccgtag cctaacaacc caaccatcaa
     3781 tccgaccgag gtcaataaag gaaagagccg gtttggaggg gtaagccgca ggatacggtt
     3841 ccacatttgg ttgttgtcct ttcttaaaag tatcgggaag cgcacacatc ctgactgttt
     3901 tgcaacagca caggaactcg ataaatctca aggactgaga actgagggct gaggactgag
     3961 aaaacaccaa gttgtttttg tcattcagga ctgactttca gaaaacaaac tcagtcctca
     4021 gtccttggag ttcagtcctc agtccccggt tttgagctac ttggtccgaa taacggtaaa
     4081 tggagttgcg ctggtggcgg ttccggtcgt gctgacgacc gaaatcggtc cggtcgtggc
     4141 tccaaacgga acggtggccg tgatttgcgt atttgagtca atcgtaaagg tggccggctg
     4201 gttgttaaag attacctgcc ggaccgttcc cagattgaat ccggaaatcg tgacactggt
     4261 tccaaacgga ccgctgggtg gcgtaaagct cgcaataatc gggccttttc cgtaagcgtt
     4321 cacattcagc acaaacgtcc gcgtggaggt ggctccacag ttgtcggtgg cggtcaccgt
     4381 gatggtgaaa ctgccgc
//
LOCUS       DAOQDO010000200         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13533_length_4394_cov_3.571791, whole genome shotgun sequence.
ACCESSION   DAOQDO010000200 DAOQDO010000000
VERSION     DAOQDO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13533_length_4394_cov_3.571791"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            748..2166
                     /locus_tag="PKZ53_04450"
     CDS             748..2166
                     /locus_tag="PKZ53_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ39718.1"
                     /translation="MSIRQRFCACLVAICLSGVFVFSQPAQAQIKPPVLKITQTKLKN
                     NLTIVLHEDHTAPIVNVQVWYHVGAKDEKPGQSGFAHLFEHIMFKGTKHIAQEEYPRL
                     IEETGGIQNAGTLQDYTFYWQTVPANYLERVLWMESERMKSLTVNEENFASERKVVLE
                     EKKLRFDNTPLGKIYALLLSNVFKTYPYAHLPIGSEQDLNAATVADVKAFHDRYYVPD
                     NATMIIVGDFKTAEVVKQIGKYFDEIPASATPVPRIKLDEPEWNGERRAIEYDARLPF
                     GGVTIAYPTVGANHPDRHALELLSNILARGETSRLYRSLVYDKQLAIFAAGRNEPLEL
                     AGIFSFQAAMTVGKPAEDGEAALLAEIEKVKTEPVTEQELNRSKAQLISSLVRQRQTV
                     QGKADNIGYATVIMGDYQLVNSELQKYQAVTVKDIQRVAQTYFNPQKRLVIYFLPESM
                     RPKQSSAQPTSTPTTETQKGAN"
     gene            2171..4348
                     /locus_tag="PKZ53_04455"
     CDS             2171..4348
                     /locus_tag="PKZ53_04455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ39719.1"
                     /translation="MTQLNSFQNSKPLRRYSLCAVVLLAITLAAPLSAVTLAAQDKPP
                     VAPPFQARPFPAFVDQTLPNGLRVLIVEDHRQPLVSLQLSLSGGAIYDADQKVGVASM
                     TADLLTKGTTTRKAKELAQIVDDLGGALGANAGDEYARVSASFLSNSTALALELLADV
                     TLNPTFDEEELNRAKTQAISGFQFTAISGAYLAGGVFDRLLFGNTPYGRPSNGTPETI
                     STISRADVVEFHRKQYTPRGAILVVTGDVQPKAVMALITKQFGGWKGEAAPPLVIQPP
                     ADPKGLRIVVVDKPDAVQTEIRVGRQAFPITDPDRITASLMNVTLGGGLFTTRLMQEV
                     RVKRGLTYGARSSLQLLTKGGKFTLSTNTKTESTVEVVRILLDELKKLRDVPISDEEL
                     AQRKSFMIGQSVLNAETPADVADNLLDAIRQGGGKQDLDTYAQKVSAITAGAIQKVMR
                     DRLNPDSSTIVLVGNAAGFEKELASLGKFEKIPFRDVDLMAANLRKPSPLERPASEAE
                     KTAGAALLKQAVAALGGEAYVNQKTVIRQGSGKFAPPGLPQPLAVPSITNTTGEGGKS
                     VLTIKHPAFEVSFGTVGDGKIWVVSPQGVQEFPNPEQGKFGIDILRRAAAHPETFLIR
                     SLPDEEKEGQKYKVFAISDQDKHTTLFYCDPETSLLSRLAYKGQQGDTEELYADYADF
                     SGVKVARKVTYKTNGAMFLDATFSEVKVNESVPDSVFAKPAGK"
BASE COUNT         1115 a         1163 c         1123 g          993 t
ORIGIN      
        1 tgtcacgtga aatgtaaatt gccaaccaga ggcgttcggc gtgaaagcag aataactaaa
       61 ttttgaattt agcggaaaaa tatatgacct gaactggcta ggaccattta aaatatttat
      121 ttacaatatt ttaaatcgtt gtgaacgcgt tcacaaatca agaaactggg cctgatgtcc
      181 aaatagtaga cctttcccac ctggtcggct tcccacactg ccctcgaaaa ccaccgatga
      241 aaccgctcgt gatgcacctc ccccaccact cccgctgcct cgatcattcc ggtgattgtg
      301 tgtcgcttca caccttgaat ccacccactg aactctctcc gggcattttt tggtgaattg
      361 cacttttcca aagtataccc aacgcgattg tcgaaggctg cctctccatc ccattcattt
      421 ttcaaaagag caaaactcca ggaaccacga aacacacgaa aaaaaccaac catttccaaa
      481 attcaatcgc tcaggaaact tttgacacgg tacttatcaa ccaccatcta cgtcaatttg
      541 gcgaataacg gctccgccgg attgaacaaa acagtctgga gtggtaaatg gtgatggctt
      601 tccgtcgcat tctttttttc aggacgtctt caggtagata ggctctgtct ccgtacattt
      661 cagtgatttg gcttgcgaag ccgatggcgg ctcgtcgcct ctatctgccc gtggagtcct
      721 tatttatcca ataaggagtc gcaccccatg tctattcgac agcgattttg tgcctgtctg
      781 gttgcgatct gtttgagcgg tgtttttgtg ttcagccaac cagctcaagc ccagatcaaa
      841 ccacccgttt taaaaattac tcaaaccaaa ctgaaaaata acctgacaat tgtgctccac
      901 gaagaccaca cggccccgat tgtcaacgtt caagtctggt accacgttgg cgccaaagat
      961 gaaaaacccg gccagtccgg atttgctcac ctgtttgagc acatcatgtt taagggcacc
     1021 aaacatatcg cccaggaaga atacccacgg ctgattgaag aaaccggagg gattcaaaat
     1081 gccggaaccc tgcaggatta cacgttctac tggcaaacgg tcccggcaaa ctaccttgaa
     1141 cgggtattgt ggatggaatc cgaacggatg aaatcgctga ccgtcaacga agaaaatttc
     1201 gcttcagagc gcaaagttgt attggaagaa aagaagcttc gctttgacaa taccccgctg
     1261 ggcaaaatct atgcgctctt gctatccaat gttttcaaaa cctaccccta tgcccacctg
     1321 ccaattgggt cagaacaaga tttgaatgcc gcgacagtcg ccgatgtcaa agcgtttcat
     1381 gatcgctact acgtgccaga caacgccacc atgatcattg tgggcgattt caaaacggct
     1441 gaagtggtaa agcaaattgg gaagtacttt gacgaaatcc ccgccagcgc cacaccggtg
     1501 ccgcgcatca agctggatga acccgaatgg aacggtgaac ggcgcgctat cgaatatgac
     1561 gcccggctgc cgtttggtgg cgtgaccatc gcctacccga ccgttggcgc caatcatcct
     1621 gaccgccatg cgctggaatt gctttcaaat attctggctc ggggtgagac gtcccggctc
     1681 tatcggagtc tggtgtatga caaacaactg gcaatttttg cggctggacg aaacgaacca
     1741 ctcgaactgg ctgggatttt ttcatttcag gcagccatga cggttggaaa accggctgag
     1801 gatggcgaag cggccctgct ggctgaaatc gagaaggtca aaaccgagcc cgtgaccgaa
     1861 caagaactca atcggtcaaa agcccagttg atcagttcac tggtccgtca gcgtcaaacc
     1921 gtccagggga aagctgacaa tatcggttat gccacggtga ttatgggtga ttaccagctt
     1981 gtcaactctg agcttcaaaa gtatcaggcg gtaaccgtga aagacattca gcgggtggcc
     2041 caaacctatt tcaacccaca gaaacggctg gtgatttatt tcctccctga gtcaatgcgc
     2101 cccaaacaat cgtcagcaca gccaacctcc acgccgacaa ccgagactca gaaaggagcg
     2161 aactaacccc atgacacagt tgaactcatt tcagaattca aagccgttgc gccggtattc
     2221 gctgtgcgcg gtggtgctcc tggcgattac cctggcggcc ccactttccg ccgtgaccct
     2281 ggcggcgcag gataaaccac cggtggcacc gccgtttcag gccaggccgt tcccggcctt
     2341 tgtggatcaa acattgccta atggtttgcg ggttttgatc gttgaagatc accgtcagcc
     2401 gctggtttct ctgcaattgt cgctctccgg cggggcaatt tacgacgctg accagaaagt
     2461 gggtgtggct tcgatgaccg ccgatttgtt gaccaagggc accaccaccc gcaaagccaa
     2521 agaacttgcc caaatcgtgg atgatctggg tggagcgctg ggtgctaatg ccggtgatga
     2581 atatgcccgc gtatcggctt cctttctctc aaactcaacc gcactggcgc ttgaacttct
     2641 ggcagatgtc accctcaacc cgacgtttga tgaagaggaa ctcaatcggg caaaaaccca
     2701 ggccatttct ggattccagt ttacggccat ttctggtgcc tatctggccg gcggggtctt
     2761 tgaccggttg ctgtttggga atacccccta tgggcgacca tcaaatggaa ctcctgaaac
     2821 aatctccacc atttcgcgtg cagatgtggt cgaatttcat cgaaagcagt acacacccag
     2881 gggggcgatt ctggtggtga ccggcgatgt acagcccaaa gcggtcatgg cgctcattac
     2941 caaacaattt gggggatgga agggcgaagc cgcaccgccg ctggtgattc agcctccagc
     3001 cgaccccaaa gggctacgca tcgtcgtggt tgataagcca gatgcggttc agactgaaat
     3061 tcgcgttgga cgtcaggcat ttcctatcac ggacccagat cggattaccg cgtcactgat
     3121 gaatgtcacg cttggcggcg gattatttac cacgcgtctg atgcaggaag ttcgcgtcaa
     3181 acgcgggttg acctacggcg cacggtcatc gctccagctc ttgaccaaag gcggaaaatt
     3241 cacgctttca accaacacca aaaccgaatc cacggttgaa gtggtgcgca ttctgcttga
     3301 tgagttgaaa aagctgcggg atgtgccgat ttctgatgaa gaactcgccc agcgaaaatc
     3361 atttatgatt ggccagtcgg tcctcaacgc cgaaaccccg gcggatgtgg ctgacaacct
     3421 gcttgatgcc atccgacaag gaggtggaaa acaggatctg gatacctacg cccagaaagt
     3481 gtctgccatc accgccgggg cgattcaaaa agtgatgcgt gatcggctca acccggattc
     3541 aagcaccatc gtgctggtgg gcaatgccgc cggatttgaa aaagaactgg cgtcactggg
     3601 gaaatttgag aagattccat ttcgggatgt ggatttaatg gccgccaatc tgcgcaaacc
     3661 atcaccgttg gaacggccag cctccgaggc ggaaaaaact gccggggcgg cgttgttgaa
     3721 acaggccgtc gcggcattgg gtggcgaggc gtacgtgaac caaaaaacgg tgattcgaca
     3781 gggaagcggc aaatttgccc ctccagggtt gccgcagcct ttggcggtgc cgtcaattac
     3841 caacacgact ggggagggcg gtaaatcggt gctcaccatc aagcatccag ccttcgaagt
     3901 cagctttgga acggttggag atggaaagat ctgggtggtt tcaccccagg gcgtccagga
     3961 atttccaaat ccggaacaag gcaaatttgg cattgatatt ttgagaagag cggcggccca
     4021 tcccgaaacg tttttgattc gcagtctccc ggatgaagaa aaagaaggtc agaaatacaa
     4081 agtgtttgcg atttcagatc aggacaaaca caccaccctg ttttactgtg acccggaaac
     4141 cagtctcctg tcgcgactgg catataaagg gcaacaggga gacaccgaag aactatatgc
     4201 tgattatgct gatttcagcg gggtgaaagt tgcccggaag gtgacctaca agaccaacgg
     4261 ggcgatgttc cttgatgcca cgttctcaga ggtcaaagtc aacgaatcag ttcctgactc
     4321 agtgtttgcc aaaccggctg ggaaataggg ttccgggttc cgggttccgg gttccgggtt
     4381 ccgggttccg ggtt
//
LOCUS       DAOQDO010000201         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13564_length_4388_cov_2.663974, whole genome shotgun sequence.
ACCESSION   DAOQDO010000201 DAOQDO010000000
VERSION     DAOQDO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13564_length_4388_cov_2.663974"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKZ53_04460"
     CDS             complement(<1..258)
                     /locus_tag="PKZ53_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNJ39720.1"
                     /translation="MSSPSHTAAQAIFVGFETYQTTFHTITLRAQTRFEQRDWLGMQS
                     DAVERLNLYKATVDQVVANVRAVLGDDLETISSLLSNMFTQI"
     gene            complement(350..1663)
                     /locus_tag="PKZ53_04465"
     CDS             complement(350..1663)
                     /locus_tag="PKZ53_04465"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39721.1"
                     /translation="MSSSSSTHPSFQAEIAEALRGLEILIEHTTVHILAFLRYQRTSE
                     RETALTLLREHLKLPVWEFRLSPEKDDPIKLLFEVPPAPRACVIFYDVENALPRLAGY
                     VNLQRERLTEVPHAVLFCVREYGLREIGLHAPDFFSWSSGTWDITTHLPAQEIELPLM
                     PLDDLSGFRNREEAEQKLHLYQELYDEYSRQEQPDQVFLADTLQKQALALYFLGRYKE
                     SVPLLEQSLTMYESHLGLNHQKTVAGLTALAVLYGLKGSFIEAEKMLNQAVELSAQFD
                     PATVVNSLQALSALYNSQKKFKEARDLLDRALTICRKSFGNHHPTTARSLSYLGAWAY
                     NQMRYTEAIAYLEEALTICEKVFGRQHPATGQILNNLAVLYVAQGKFETASKFYEQGL
                     QIWKTRLGEDHPFTKILIGNYADLLWNLGRTEEARTLEGKVADTG"
     gene            complement(1681..2913)
                     /locus_tag="PKZ53_04470"
     CDS             complement(1681..2913)
                     /locus_tag="PKZ53_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39722.1"
                     /translation="MKASTLEAALNLLNPDHALSTAEELDEFFVSRSASPSKYVKVLI
                     SQSQAQEKFLFTGHRGSGKSTELAKLVVDLLQSTEPFFFPVNYSVKSVLNLFDLTYVD
                     VLLSIGLQLFEKVVQQNMQVSQTVLTNILNFTKTLSTEVQMGIAAQAETGLEIGLYAT
                     KLSTKLRVEDSTRLTVRETLSHRISDLLENIDLLVREIERTTHRRTVIIVEDLDKTDL
                     GVAKKLFYEYASSLLSPQVRIIYTFPTALRHDNDFMQVGSNFSNTFIFPNLKLDQFDG
                     TPDTTGRAQLREMITRRVEAQLFDNEALDELVQSSSGLPRELLALGRRACLDALVQGK
                     TRIQIDQVKLAVGAKRRDYQTLLTTEQLDLLSEIKTTRRIKNDGAHQALLHNLSVLEY
                     RNETVWYDVNAIVKALLP"
     gene            complement(3034..3689)
                     /locus_tag="PKZ53_04475"
                     /pseudo
     CDS             complement(3034..3689)
                     /locus_tag="PKZ53_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(3686..>4388)
                     /locus_tag="PKZ53_04480"
     CDS             complement(3686..>4388)
                     /locus_tag="PKZ53_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ39723.1"
                     /translation="LRGGCQQHALRIEDQAIVEERTRIARELHDTLGHLLVAQHIQLE
                     NALLSLQSNSTADLAVFLQESQKLTSKALKNVRQAVTILRDESEEFIPFEAAIAEIVE
                     EFHQMSPIRIQSNITLSAQLPQSLQLAARRILTEALTNIHKHSQATDASVTIEIKPFT
                     TAKALDEPLVETALWVSVSDNGCGIHQPESASGFGLRGMRERVQSFGGRLTIESQPGA
                     GCHLSAVLPIQGGTR"
BASE COUNT         1034 a         1066 c         1144 g         1144 t
ORIGIN      
        1 gatctgggtg aacatgttgg aaagcaggga cgagatggtt tccagatcgt cgcctaaaac
       61 cgcccggaca ttggccacca cctggtcaac tgtggcttta tagaggttga ggcgttcaac
      121 ggcatcgctt tgcattccaa gccagtcccg ttgctcaaaa cgtgtctggg cacgcagcgt
      181 gatggtgtga aaggttgtct gataggtttc aaaacccaca aaaatggcct gggccgcagt
      241 gtgtgatggg gaactcatgg tggatggatt gaaatttgag gctgctgaag atatcggggc
      301 aagtgtgagg tgcaaagtag ggtattttct gattttgctc aacaggattc tacccggtgt
      361 cggcgacttt tccttccagc gttctggctt cttcagttcg tcccaggttc cagagtaaat
      421 cggcatagtt ccctatcaaa attttagtaa agggatggtc ttctccaagc ctggtcttcc
      481 aaatttgcaa tccctgctca tagaatttgc tcgctgtttc aaatttcccc tgagcaacat
      541 aaagcacagc taaattattc aaaatctgac ctgttgccgg atgctgtctt ccaaagactt
      601 tttcacatat ggtcagggct tcctcaaggt aagcaattgc ttccgtatac ctcatctgat
      661 tgtaagccca ggcgccaagg taacttaaac tcctggctgt tgttgggtgg tggttgccaa
      721 atgactttct acaaattgtc agtgctctat ccaacaagtc acgagcttct ttaaatttct
      781 tttgtgagtt gtataaagcc gacagtgcct gcagggagtt tacaactgtt gccggatcga
      841 attgcgcact cagttccacc gcctggttga gcattttttc agcctcaatg aaggacccct
      901 tcaatccata tagaaccgcc agggcggtta gcccggccac cgttttttga tggttgagcc
      961 caaggtgtga ttcatacatt gtgagcgatt gttccagcag gggaacactt tctttgtatc
     1021 gcccaagaaa gtacagggct aaagcctgtt tctgtaatgt atcagccaga aaaacctgat
     1081 ccggctgttc ctgccgactg tattcgtcat acagctcttg atacaaatga agtttctgtt
     1141 cagcttcttc acgatttctg aagccgctca ggtcatcaag cggcatcagg ggaagttcaa
     1201 tttcctgagc tgggaggtgg gtggtgatgt cccaggtgcc gctgctccag gaaaagaaat
     1261 caggcgcgtg aagcccgatt tcccggagtc catattcccg aacgcaaaaa aggaccgcat
     1321 gcgggacttc ggtcagtcgt tcccgctgaa gattgacgta gccagccagc cggggcagtg
     1381 cattttccac atcgtaaaaa atgacacagg cgcggggcgc tggtggcacc tcaaacagca
     1441 atttgatcgg gtcatctttt tccggggaca gtctgaactc ccaaaccgga agcttcagat
     1501 gttcacgaag caacgtcagg gcggtttcac gttcggaggt tctttgatag cgaagaaagg
     1561 ccagaatgtg gacagtggta tgttcgatca ggatttccag cccacgaagt gcttcggcaa
     1621 tttcagcctg aaatgatggg tgcgtggaac tggaacttga catcagaatg tctccgaaac
     1681 tcagggaagc agggctttga cgatggcatt gacatcatac cacaccgtct cgttgcgata
     1741 ttccagcacg ctcagattat ggagcaacgc ttgatgtgcc ccatcatttt taatccgccg
     1801 ggtcgtttta atctccgata agaggtcaag ttgctcagtt gtcagcaagg tctggtaatc
     1861 ccgccgtttg gccccgaccg caagttttac ctgatcaatt tggattctgg tttttccttg
     1921 aaccagagcg tcaagacaag cccgacgacc aagcgccagt aactcgcggg gaaggccgct
     1981 acttgactga accagttcgt caagcgcttc attgtcaaag agttgtgcct caacacgtcg
     2041 ggtgatcatt tcccggagtt gggcccgtcc ggtggtatca ggagttccat caaactgatc
     2101 cagtttcaga tttggaaaaa tgaatgtgtt tgagaaattc gatccgacct gcataaaatc
     2161 gttatcgtgt ctcagggcag tcggaaacgt gtaaatgatt ctgacctggg gcgagagcaa
     2221 cgacgaggca tattcgtaaa acagcttttt ggcgacgcca agatctgttt tatcaagatc
     2281 ttccacgatg atgaccgttc ggcggtgggt ggttcgctca atttcacgca ccagtaaatc
     2341 aatgttttcc aataaatcgg agatgcgatg actgagcgtc tcacgaacgg tcaggcgggt
     2401 actatcttca acccgcagct tggttgatag tttggtagca tacaacccaa tttccagacc
     2461 agtctcagcc tgggcagcaa ttcccatctg aacttcagtt gaaagggttt ttgtaaaatt
     2521 caaaatattt gtcaataccg tctggctgac ctgcatattt tgttggacca ctttctcaaa
     2581 caattgcagg ccaatggaca acaaaacatc cacgtaggtt aaatcaaata aattcaagac
     2641 acttttgact gaatagttga ccgggaaaaa gaaaggctcg gttgattgga gtaaatctac
     2701 aacgagcttc gcaagctccg tgcttttccc acttcctcga tgaccggtaa agagaaactt
     2761 ttcctgagcc tgagattggg aaatcaggac cttcacatat ttgcttgggc tggcggaacg
     2821 cgagacaaaa aattcgtcga gttcttccgc tgtgctgagc gcatggtcag ggttcaacag
     2881 gtttaatgct gcttctaagg tactggcttt cataggctta acaaggtctt tccggctgaa
     2941 aaatattgaa ggaattgaga cgagccagca ggatagccaa ccctggaccg gggtgcaact
     3001 tgatcaaaac ccggtcgtga cgatgggttc aggttacgat ttgggcggag gcaaaaatga
     3061 aagcgcaaag acagcggcct gagtccggtc acgcagattg aggcagctca aaatccgggt
     3121 gatgtggttc ttcacggttt tttcactgat aaacaaaatt tcggcaattt cgcgattact
     3181 ggcgccggtt gcaatcaggc tcaatacttc gtgttcgcgg gggcttaatg ctgccagggc
     3241 cgcctttggg ccttcggatg aggtcagttt tggaattgac tggcgcggca acactttttt
     3301 cagcaggcct ggcccaagct gggtatagcc tctggcgacg gcccggatgg cctcagccag
     3361 ttcttttgaa ggtgtgtctt tgagcaatag ccggttgcac cgaaccgcat tgcctgttca
     3421 atataggttt catcgtcaaa cgttgtcagg accagaattt tcgtcgtcgg gtgccgttcg
     3481 atgatgtggc gcgtggccgc aaccccatcc ataaccggca ttcgaatatc catcaagatg
     3541 acgtccggtt tgagtttggc catctggtca caggcttcct gtccattggc tgcctcaccg
     3601 accaccatca aatccgggtg gatttcaagg agtcgccgta atccttgccg gacaatcatc
     3661 tggtcttcca caatcatcag tcggatcatc gggttcctcc ctgaatcggt agcacggcac
     3721 tcaggtgaca gcccgcgccg ggctgacttt caatggtgag cctgccccca aaactctgca
     3781 cccgttcccg cataccccgc aacccaaacc cggaggcgga ttcaggttga tgaatcccac
     3841 atccattgtc cgagactgac acccacaggg cggtttccac aagcggttca tccagggctt
     3901 tcgctgtggt aaatggtttg atctcaatgg taaccgaggc gtcggttgcc tgactgtgtt
     3961 tgtggatatt ggtcagggct tcggtcagga tccggcgtgc ggccagttga agactttgag
     4021 gaagctgggc tgaaagtgtg atgtttgatt gtatcctgat gggcgacatc tggtgaaact
     4081 cctccacaat ttcggcaatg gctgcttcaa atgggatgaa ctcttcggat tcgtcgcgca
     4141 ggatcgtgac tgcctgccgc acgtttttga gtgccttcga tgtcagtttc tggctttcct
     4201 gcaggaacac agccaggtcc gcagtggaat tggattgaag cgaaagcagt gcattttcaa
     4261 gctggatatg ttgtgccacg agcaagtgtc caagcgtgtc gtgcagttca cgggcaatcc
     4321 gggtgcgttc ttccacaatg gcctgatctt cgattcggag ggcgtgctgt tgacatcctc
     4381 cccgcaat
//
LOCUS       DAOQDO010000202         4384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13583_length_4384_cov_2.757681, whole genome shotgun sequence.
ACCESSION   DAOQDO010000202 DAOQDO010000000
VERSION     DAOQDO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13583_length_4384_cov_2.757681"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1597)
                     /locus_tag="PKZ53_04485"
     CDS             complement(113..1597)
                     /locus_tag="PKZ53_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNJ39724.1"
                     /translation="MVAPLKKQNPIHSLEVHSTLAIEIERRRFSRLVFTRRAVQVIWA
                     LAPVILSCWRDVHRYWFFGKPLNRSESHHARRAITLRKKIEHLGVSFIKVGQVLSTRG
                     DLFPKVYIDTLSTLQDSVNPLPFELVRTELEGVYGQSLAAIFDTFSEKPLASASMGQV
                     HRATYAGRDVVVKLVRPGIEPLVWIDYALIQAILNVLESQCSRLTASTSDIQAFLRLF
                     RQVVTEVHRGMLEEIDFHHERLNAERLARLLATRPGVKVPQTVPELCHPTVLVLEYLA
                     GTKISELETLRHQGFDPLKLVDQVVELYVEMILVNGVYHGDPHPGNVFVSPRGEIILF
                     DFGIVRELSQAMRDGLLATVLAGVRGEVDGVVDELYRLGILDPAANRETALIVARKIA
                     ELHFQGISTADRIETTRRFIRNAFGYLPLHLPQELVYVFRVTSMLEGLGTRVKPGWNM
                     MADGGPAIRRVVGHLLVQHTSVSWLDTVRSWAKRLWDSFFGQSS"
     gene            complement(1758..2594)
                     /locus_tag="PKZ53_04490"
     CDS             complement(1758..2594)
                     /locus_tag="PKZ53_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNJ39725.1"
                     /translation="MAKKLANPHLRTLENTLSSTYGWALTPAFRAMLQEVVSSKAQRL
                     GLDEITYCRMAVKSGGELQSVAEGVISRETLFFRDPEQYRALHTLVLPALIERRQNDR
                     KLRLWSAGCSTGEEPFSLAMLVECLLTDRENWRVEILAVELSSKLLMEANLGRYPNSQ
                     LVNVEAEFRERFFSTPPGGTTGLLELDRRVRRNVKFRHANLYDAQLWQFLPGQFDLIV
                     CSQVLCQMHHTGIHQTLGRIHQALSPHGYFMVSPTEASLVVSSRLKSARALPTGFFMK
                     AE"
     gene            complement(2718..3431)
                     /locus_tag="PKZ53_04495"
     CDS             complement(2718..3431)
                     /locus_tag="PKZ53_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ39726.1"
                     /translation="MPEPKRILLVDDEPQITRVLRRALMARGYEVRTAAEGVEALDVF
                     KTWMPDLVVTDLSMPNMGGLELCERLRAVSTVPIIVLSVKGDERTKVEALDAGADDYV
                     TKPFGIDELLARIRAALRRVATPVTEPKTTLTAGDFVIDLEARRVLINHLEVHLTPKE
                     YDLLAYLLRHAGKVLTHRTLLAAVWGGNYVEQTEYLRVFIGQLRKKIEPDPSRPRYIL
                     TEPWVGYRFQTEPNSEVVK"
     gene            complement(3400..>4384)
                     /locus_tag="PKZ53_04500"
     CDS             complement(3400..>4384)
                     /locus_tag="PKZ53_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39727.1"
                     /translation="LPPVRTFTIADPDNWVALGAFLVTAVTAGHLSASVRQRAEEAET
                     RRLEIEHLYRELQAAFERESHLEAIRQSEQLKSALLDAVTHDLRTPLTSIKVSVTTLL
                     DETEPGAEFALDREGQHELLQIIDEETDRLNRFIEGLVGLARIEAGKFSLRRTWNAVD
                     EVIAAALHRAESQTRQHRIILELAEELPSVRVDAHALAEVIYTLVENAAKYSPPDTTI
                     AISAKTLPGEFVQISIEDQGIGVPAEEHEKVFEKFYRVASTHGNEPGHPTSLGLGLSI
                     AQGIVEAHGGKIWMTAGYGGKGTCVSFTIPIGDDEPEGPGHNAGTETDSAG"
BASE COUNT         1107 a         1206 c         1102 g          969 t
ORIGIN      
        1 accaagtacc aagtaccaag taccaagtac caagtaccaa gtaccaagta ccaggcacca
       61 agtaccaagt accaagtacc aagtaccaag tatcaagtac caaacccgtc tttcaggacg
      121 actgaccaaa aaatgaatcc cacaaacgtt tggcccagga gcgaaccgta tccagccagg
      181 aaacagatgt gtgttgaacc aggaggtgtc cgaccacgcg gcgaatggct gggccgccat
      241 cggccatcat attccaaccg ggtttcactc gggtgccgag accttcaagc atcgaggtga
      301 cacgaaacac atacaccagt tcttgcggca gatgcaacgg cagatagcca aatgcgttgc
      361 gaataaagcg acgtgtggtt tcaatccgat ctgccgtgct gattccttga aaatgcagtt
      421 ccgcgatttt gcgggcgaca atcaatgctg tttcgcggtt ggccgccgga tccagaatgc
      481 ccaaccgata cagttcatcc accacgccat ccacttcgcc ccgaacacca gctaaaaccg
      541 tggccagtaa accatcgcgc atcgcctggg aaagttcccg cacaatgcca aaatcaaaca
      601 ggatgatctc acctcgtgga ctgacaaaaa cgttcccagg gtgtggatca ccgtgataaa
      661 cgccgttgac caaaatcatt tcgacataga gttcaaccac ctgatcaacc agtttcaacg
      721 ggtcaaaccc ttgatgccga agtgtttcca gctccgagat tttggttcca gccagatatt
      781 caagcaccag caccgtcgga tgacagagtt ctggcactgt ttgaggtact ttgacgccag
      841 gccgcgttgc cagaaggcga gcgagccgtt cagcgttgag acgctcgtgg tgaaaatcaa
      901 tttcttcgag cattccacga tgaacttcgg tcacaacctg tcgaaacagc cggagaaacg
      961 cctggatgtc cgaggtactg gccgtcaacc gggaacattg tgactccaga acattcaaaa
     1021 tcgcctgaat cagggcgtaa tcaatccaca ccagcggctc aataccaggc cgaaccagtt
     1081 tgaccaccac gtcccgaccg gcatacgtcg cccgatggac ctgccccatt gaagccgaag
     1141 ccagcggttt ttctgaaaaa gtgtcaaaga tcgcggccag cgactgcccg tacacacctt
     1201 ccaattcagt gcgaaccagt tcaaatggca acgggttgac actgtcttgc agtgtactca
     1261 acgtgtcgat atagactttt gggaataaat ctccgcgagt actcagaacc tgtccgactt
     1321 tgataaagct cacgccaagg tgttcaattt tcttgcgaag agtgatagcg cggcgtgcat
     1381 gatgcgactc gctccggtta agtggctttc caaaaaacca gtagcgatgc acatcacgcc
     1441 agcatgaaag aatcaccggg gccagagccc agatcacctg gactgcccgc cgggtaaaaa
     1501 ccagacgtga aaagcgtcgg cgctcaattt cgatagccag tgtcgaatga acttcaagag
     1561 agtgaattgg attttgtttt ttgagagggg caaccaagta cgttttccag gcagttgcag
     1621 gacatcttct attcggcttg attctaatga cttagatgga atgtgacaag tgagagcaag
     1681 agcaaagagc gaagagcgag agagtaaggc gtgaggagca agtcgcgaag agtcactcct
     1741 ggttctaacc ttgcgactca ctcagccttc ataaaaaatc cagtcgggag cgcccgggct
     1801 gatttgaggc gggaagaaac caccagtgat gcttcggtgg ggctgaccat aaaataacca
     1861 tgcggtgata atgcctgatg gattcgcccc agcgtctgat gaattccggt gtgatgcatc
     1921 tggcacaaca cctgcgaaca cacaatcaag tcaaattgcc ccggcaaaaa ctgccagagt
     1981 tgagcatcat agagattggc atgccgaaat ttgacattgc gccgaacacg ccgatccagt
     2041 tccagcagac cagtcgtgcc acctggaggt gtcgaaaaaa accgctcgcg gaactctgcc
     2101 tccacattga ccagttgtga gttgggataa cggcctaaat tcgcttccat caacagttta
     2161 cttgaaagtt caaccgccag gatttccact cgccagtttt ctcgatcagt gagcaggcac
     2221 tcaaccagca tcgccagcga aaagggttct tcaccggttg aacacccggc actccagagt
     2281 cgcaattttc ggtcgttttg gcggcgttcg atcaaagccg gaagcaccag cgtgtgcagt
     2341 gcccgatact gctctggatc tcgaaaaaaa agtgtttcgc ggctaatcac gccttccgct
     2401 actgattgga gttcgccgcc tgatttgacg gccattcggc aataggtgat ttcatccagc
     2461 cccagccgtt gggctttgct gctcacaact tcctgcaaca tggcgcgaaa ggccggtgtc
     2521 agcgcccacc cataggtgct cgacaacgta ttttccaaag tccgcagatg agggttggcc
     2581 agcttttttg ccattgaatc aaaaagtgaa ggagcgaaag agtgagaacg cgaaggagcg
     2641 aagtgtcagt tctctatgtg ccactgtcaa caatgaatcg ccagaatcac tgaattctat
     2701 tccatttcac gacttcacta tttcacgact tcactatttg gctctgtctg aaaccgatac
     2761 ccaacccagg gttcggtcaa gatatagcgt ggccgggaag ggtccggttc aattttcttg
     2821 cgcaactggc caataaaaac ccgcaggtat tcggtttgct caacataatt cccgccccag
     2881 actgccgcca gcaacgtccg atgggtcagc actttgccag catggcggag taagtaagcc
     2941 agtaagtcat attcttttgg cgtgaggtga acttccagat gattgatcag cacgcgacgg
     3001 gcttcgaggt caatgacaaa atcacctgcc gtcaaggttg ttttgggttc ggtaaccggg
     3061 gttgccaccc gtcggagagc agcccggatt cgggccagta actcatcaat gccaaagggt
     3121 ttggtaacat agtcatctgc cccggcatcc agcgcctcaa ctttcgtccg ttcatcacct
     3181 ttgacggaca acacaatgat agggacagtg gaaaccgccc ggagccgttc acagagttct
     3241 aatccgccca tattgggcat tgacagatca gtgacaacca gatccggcat ccaggtttta
     3301 aatacatcaa gcgcctcgac gccttcagcc gcagttcgga cttcgtaccc acgcgccatc
     3361 aaggcccgtc gaaggacgcg cgtgatttgg ggttcatcat caaccagcag aatccgtttc
     3421 ggttccggca ttgtgccctg gtccttccgg ttcatcatca ccaattggaa tggtgaaact
     3481 cacacaggtg cccttcccgc catacccggc agtcatccaa attttcccgc cgtgagcttc
     3541 cacaatcccc tgagcaattg acaacccaag gcccaaactg gtcggatgcc ctggttcgtt
     3601 gccatgagtt gaagccaccc gataaaattt ttcaaacact ttttcgtgtt cttcagccgg
     3661 aacaccaatg ccctggtctt caattgaaat ctggacgaat tccccaggca gggttttggc
     3721 tgaaatagca atggtggtat caggtggtga gtactttgcc gcattttcaa ccagtgtgta
     3781 aatcacctca gcgagagcgt gcgcatcaac tcgcaccgat ggcaattcct ctgccagttc
     3841 caggataatt cgatgctgac gggtttggga ttcagcccga tggagtgctg ccgcgatgac
     3901 ttcatcaacc gcgttccagg ttcggcgaag cgaaaatttt ccggcttcaa tccgggccag
     3961 accgaccagt ccttcaataa accggttgag ccggtcggtt tcttcatcaa tgatctgcag
     4021 gagttcgtgc tggccttcgc gatcaagcgc aaattcggcg cccggttcgg tttcatccag
     4081 cagcgtcgtc accgaaacct tgattgaagt cagcggcgtg cgcaggtcgt gggtaacggc
     4141 atcaagcagg gccgatttga gttgttcgct ttgccggatg gcttcgaggt ggctttcacg
     4201 ctcaaacgcc gcctggagtt cgcgatagag gtgctcaatt tcaagtcgtc gggtttcggc
     4261 ttcctctgcc cgttgccgaa cactggctga cagatgacct gccgtgactg ccgtgaccag
     4321 aaaggcccca agcgcgaccc agttatctgg atcggcaatc gtgaaggttc gaaccggtgg
     4381 caaa
//
LOCUS       DAOQDO010000203         4383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13592_length_4383_cov_2.628466, whole genome shotgun sequence.
ACCESSION   DAOQDO010000203 DAOQDO010000000
VERSION     DAOQDO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13592_length_4383_cov_2.628466"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PKZ53_04505"
     CDS             <1..543
                     /locus_tag="PKZ53_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39728.1"
                     /translation="IQKGLFNQLFFDPLMRNRYLNTYGRQRLLAIQHGVNELVNGETT
                     PKSVIDNIAAINHHFRLRRAIEGHVRQIVRSIHKAPLDKNEQTFLRNTVNRQLEQDGL
                     IQEPIPDELFASVLIALREEGLYLQELLPRIIESQDRQLRDDFLENSGLDRFRIEEME
                     QKYLKEKNLPANTLDFLAGD"
     gene            694..1464
                     /locus_tag="PKZ53_04510"
     CDS             694..1464
                     /locus_tag="PKZ53_04510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNJ39729.1"
                     /translation="MLSFAKQLAKDAGKILREHVGRDFEVRHKGQIDLVTEVDLLSEK
                     FIRDQIANHYPKHQVLAEESGLFEQTSPYRWIIDPLDGTTNYAHGYPFYSVSIALQHE
                     SHVLLGVVYDPVRDELFAAERGQGATLNERAIRVSTVERLDQALLCTGFPYNVRTSPK
                     INLDHFSRFLDAAQAVRRDGSAALDLCYVAAGRFDGFWELNLAPWDMAAGGLIVTEAG
                     GTVTRFDHGTFDWYVPEVLASNGLIHQEMSAVLVQKGT"
     gene            1648..2244
                     /locus_tag="PKZ53_04515"
     CDS             1648..2244
                     /locus_tag="PKZ53_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNJ39730.1"
                     /translation="MSGNSLVAPEDLIDYALDLGLAGIGVTERNSYTASEVCVEFAEG
                     LPLLVLRGLEINTDLGEMLIYGLTELDAQVFERPGKWIAMEAVEYVRFHGGICIPAHP
                     FDPDRPSIGEKLTTLPGIFAIEGLNGRTPDLNNQEACRFADMMRLKVIGGSDALNLGQ
                     IGRCVTEFEQPIRDEIEFLEELRAGRYTARYFIQHPSL"
     gene            2268..2879
                     /locus_tag="PKZ53_04520"
     CDS             2268..2879
                     /locus_tag="PKZ53_04520"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39731.1"
                     /translation="MSTEVKKRRYTLEEYYALEQKSEGRYEFWNGEVYAMAGASPEHE
                     LILGNLIAELKQRLRGRSCVVYSSNLRVKVPELPPYRYPDLTALCETPEFVEIGGVKT
                     LVNPALIVEILSPSTEAFDRGDKFTLYKSIPSFCEYLLIAQHRPHVSQYVKQANSAFW
                     MFHEVNALSATVDLKSVNCTLDVSELYQGVEFSTSQPPFPITE"
     gene            2949..4319
                     /locus_tag="PKZ53_04525"
     CDS             2949..4319
                     /locus_tag="PKZ53_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNJ39732.1"
                     /translation="MANFFPRNVILISLDTLRHDCISAESNRLFLGELAPLVQTPNID
                     RLAAEGVRFSNCVSSCPLTTPSHAAMFTGMYWPKHRVFHQFETPMSARALPLAERLKK
                     HKFFTLQNSGRALGEGAMFESKHTGLRRGYEKSVFAADLQHQTIKFITKQKAPWYLFF
                     HTFNVHWPYGITPEAFDTQLASAWSSDDWTQVRRTYIENANRVDAQIGDLLAMLDQTG
                     QREDTLIVLTADHGEGLNRRVPMHGAVNGGLDEVIRVPLIFHHPRSLKGNVTVDAPVR
                     TIDIVPTILNLLGITDPYDPGFEMVDGRSLWPLMRGEVLAEKPACFCCFTYDFEADRP
                     FLRGVRTREWKLILNDCTESEINRFAERIRTKMQWPLKDEVRLEGMRDILKRESFVEL
                     YDLRQDPFEQTNVAEANPEVVNHLVAELAQVDPGVRINQRSSSSLSKEELDILKQQLV
                     DLGYMI"
BASE COUNT         1057 a         1040 c         1180 g         1106 t
ORIGIN      
        1 atccagaagg gcctgttcaa tcaattgttt tttgatccgc tgatgcgcaa tcgctatctc
       61 aacacatatg gtcggcagcg cctgctggcc attcagcatg gtgtcaatga actggtgaac
      121 ggggaaacca cacccaaatc ggttattgat aatattgcag caatcaacca tcattttcga
      181 ttacggcggg ctatcgaagg acacgtgcga cagattgtgc gctcgatcca caaagctcca
      241 ctcgataaaa acgaacagac ttttttacgc aacacggtca atcgtcaatt agagcaggac
      301 ggactgattc aagagccaat tccggatgaa ttgtttgcgt cagtgttgat tgctttgcgt
      361 gaagaagggt tataccttca ggagttgctt ccgcgcatta tcgaatccca agaccgtcag
      421 ttacgagatg actttctgga aaacagcgga ctggaccgtt tccgaatcga ggaaatggag
      481 cagaaatatc tcaaagaaaa gaacttgccg gccaatacac tggattttct ggccggcgat
      541 tagtgatttg agggttgaag aaaacgggct gaagacattg ggctaggggc tgagggcaga
      601 agacatcggg ttcagggtat tgaacccgtt ttcttcagcc ccgagcttgc gagtcttccg
      661 ccctcagccc tcagccccaa aaaggatttc tccatgcttt catttgcaaa acaactggcc
      721 aaagacgccg gaaaaatact gcgcgaacac gtcgggcgtg attttgaagt ccgccataaa
      781 ggacagattg acctggtaac cgaggttgat ttgctttcgg aaaagtttat tcgcgaccag
      841 attgccaatc actatccaaa acatcaggtg ctggctgaag aaagcggttt atttgagcag
      901 acttcgccct atcggtggat cattgatcca ttggatggca cgacgaatta tgcccacggg
      961 tacccgtttt acagtgtttc gattgcgctc cagcatgaaa gccacgtgtt gctgggtgtg
     1021 gtctatgatc cggttcgcga tgaactcttt gcggcggaac gtggtcaggg ggcgacgctc
     1081 aatgaacgcg ccatccgggt atcaacggtt gagcgtcttg atcaggcatt gctgtgtacc
     1141 ggttttccct acaatgtgcg cacttcgcct aaaatcaacc tggatcactt cagtcggttt
     1201 ttggatgcag cccaggctgt caggcgcgat ggttcggctg cgctggattt gtgttatgtt
     1261 gcggcgggcc ggtttgatgg attttgggaa ttgaatctgg cgccctggga tatggccgct
     1321 ggcgggttga ttgtgaccga ggctggcggt acggtaactc gttttgatca cgggacgttc
     1381 gactggtatg tccccgaagt tttggccagt aacgggttga ttcaccagga aatgagtgcg
     1441 gttcttgttc agaaagggac ataaaggaca taaaggacga aaaggacata aatttgaaac
     1501 ccctgaaccc gataagatga ccgattgaca aggtgacagg gtgatttttt tcatccctca
     1561 tccctcatcc cttccgaaaa ccctgaaccc tgagtcccag accctcgaat tattatgatg
     1621 ctcttcgatt tgcacgtctc cactgtcatg tcgggtaact cgctggttgc gccagaagac
     1681 ttgattgatt atgccctgga ccttgggctg gcgggcattg gggttaccga acgaaattcc
     1741 tatacggcct ctgaagtctg tgtcgaattt gccgaaggcc tgccactgct ggtgctccgt
     1801 gggttggaaa tcaatactga tctgggcgag atgctcattt atggattgac ggaactggat
     1861 gctcaggttt ttgagcgacc agggaaatgg attgccatgg aagctgtcga atatgtgcgc
     1921 tttcacggtg gcatttgcat tccggcccac ccgtttgacc ctgatcgccc aagcatcggt
     1981 gaaaaattga ccactttgcc gggaatcttt gccatcgaag gtctgaatgg acgcacgccg
     2041 gatttgaata accaggaggc ctgccggttt gccgacatga tgcggctgaa agtgattggt
     2101 ggcagtgatg cgttgaattt aggccagatt gggcggtgtg tgaccgagtt tgaacaacca
     2161 atccgcgatg aaatcgaatt cctggaagaa cttcgggccg ggcgctatac agcccggtat
     2221 ttcattcagc atccttcact gtagctgcgg attgaggagg ggaagcgatg tcaactgaag
     2281 tcaaaaaacg tcggtacacg ctggaagaat attatgcgct ggagcagaag tcagaagggc
     2341 gctatgagtt ttggaacggt gaagtctatg ccatggctgg tgcttcacct gaacatgaat
     2401 tgatcttggg aaacttgatt gccgaattaa agcaacggct ccgtggccgt tcctgtgttg
     2461 tgtatagttc aaatctacgt gtgaaagtcc ctgagttacc tccctatcgc tatcctgatc
     2521 tcactgcgct gtgtgaaact cctgaatttg tggaaattgg cggtgtcaaa acgctggtca
     2581 acccggcatt aattgtcgaa attctttccc cttcgaccga agcctttgac cggggagata
     2641 aattcacact gtacaaatca attccgagtt tttgtgaata tctgctgatt gcccaacacc
     2701 ggccacacgt gtcgcagtat gtaaagcaag caaactcagc cttctggatg tttcacgaag
     2761 tgaatgcttt gtcagccaca gttgacttga aatcagtgaa ctgcacactt gatgtatctg
     2821 aactgtatca aggagttgag tttagtacgt ctcaaccgcc atttcccata accgaataat
     2881 cctgtaagtc agtaccacct gcgtcagcgg gtggtactga caggtcctct ttttttcaaa
     2941 gttgtttcat ggctaacttt ttcccacgca atgtcatttt aatcagtctc gacacgctcc
     3001 ggcacgactg tatcagtgcc gaatcgaacc gcttgttttt gggcgaactg gcgccgctgg
     3061 tgcagacacc caatattgat cggctggccg cagaaggtgt gcggttctca aattgtgttt
     3121 catcctgtcc gttgacaaca ccttcgcacg ccgcgatgtt taccggaatg tactggccaa
     3181 agcaccgcgt ttttcatcag tttgaaacgc cgatgagtgc gcgggcgctc ccactggccg
     3241 aacggctcaa aaaacacaag ttcttcacgc ttcaaaactc gggccgggcg cttggggaag
     3301 gggcaatgtt tgaaagcaaa cacacggggc tgcggcgcgg ctacgaaaaa tcggtgtttg
     3361 cggctgacct gcaacaccaa accatcaagt tcatcaccaa acaaaaagct ccgtggtatt
     3421 tgttttttca cacgttcaac gtccactggc cctatggtat tacgcctgaa gcgtttgaca
     3481 cgcagcttgc cagtgcctgg agttcagatg actggacgca ggtccggcga acttacattg
     3541 aaaatgccaa tcgcgtggat gcccagattg gggatttgct ggcgatgctt gaccagaccg
     3601 gtcagcgtga ggacaccttg attgttttga ccgccgatca cggtgaagga ctcaaccggc
     3661 gagttcccat gcacggtgcc gtgaatggtg gtctggacga agtgattcgc gttcccttga
     3721 tttttcacca tccccggtcg ctgaaaggga atgtcacggt tgatgccccg gttcggacca
     3781 tagacatcgt cccgacgatt ctcaatttgc ttggaattac cgacccctat gacccaggtt
     3841 ttgaaatggt ggatggccgc agtctctggc cattgatgcg aggagaggtt ctggccgaaa
     3901 aaccagcctg tttttgttgc ttcacctatg actttgaagc agatcggcca tttttgcgtg
     3961 gggtgagaac tcgggagtgg aagttaattc tcaatgattg caccgaaagc gagatcaacc
     4021 gctttgctga gcgcattcgg accaaaatgc aatggccgct gaaagatgaa gtacgactcg
     4081 aaggcatgcg cgacattctc aaacgcgaat catttgtcga actctatgac ttgcggcagg
     4141 acccgtttga acaaaccaac gttgccgaag ccaaccccga agtcgtgaac catctggtgg
     4201 cggaactggc tcaggttgat cctggggttc ggatcaatca acggtcgtcg agttccctca
     4261 gcaaggaaga actggacatt ctgaaacagc agttggtcga tttgggatat atgatttagg
     4321 gctgaagaag tcgggctgaa gaagtcgggc tgaagaagtc gggctgaaga agtcgggctg
     4381 aag
//
LOCUS       DAOQDO010000204         4373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13643_length_4373_cov_3.625521, whole genome shotgun sequence.
ACCESSION   DAOQDO010000204 DAOQDO010000000
VERSION     DAOQDO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13643_length_4373_cov_3.625521"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PKZ53_04530"
     CDS             <1..1328
                     /locus_tag="PKZ53_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ39733.1"
                     /translation="RNPEPGTRNPEPGTRNPIMEHLPKLELISEYPAIPARSDKTFYV
                     LVQITPPERAKESYRPPLNISLVLDRSGSMGVDKLNQAKQAACSFIDRLLPTDRLSLV
                     TFDTRVQVLVPSTLVTDKQALKAAVAEIYGGSRTALHEAWVRGGLEVSHGATSSSINR
                     VILLTDGIANVGETCPETILNHTSELTQRGITTSTIGIGFDFQEDLLIPMAEAGSGNA
                     FHVVRTTDLEPVLTAEVDNMISQIGHSTRLGFITSSALQVRETLNDFAPVHKQEVRLP
                     NLRAGHPLQIVLAIAANVKTSTRPQTVSIRLTWQAQATGDRHSVNQTLFLPIGMTSEK
                     SAHPEVRKLVGLLTIARIKREAMRQIDAERYAEARQLIFSAIQMTATLLTNVGNDFRL
                     RAQLKALDQLEHDLEQGDDPAKSRKFLAYQSYATSTCRLDSQPDRKTGS"
     gene            1551..2555
                     /locus_tag="PKZ53_04535"
     CDS             1551..2555
                     /locus_tag="PKZ53_04535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNJ39734.1"
                     /translation="MPSSTLEIPIMTDRSVHPTSQLAVKISALTDVGRVRSGNEDNFL
                     VIDLETGFTWTADSDPPENNPFTITPADSGLLCAVSDGMGGAAAGEVASKLAVAWSGW
                     IMSEACAHPDLKIYPFHERLRLAIEQANIQIAMAAEEAPEFSGMGATFTAAGIQGNSL
                     TLAQVGDSRAYLIRNGHMVQLTRDQSLVAQLIEMGSITEEEAEFHPYRNVIMQALGAN
                     AWVEVAVQVINLCQGDLVLLCSDGLSGKVRKDQMQKLITEVSHDLRLGVRKLIYAANE
                     AGGDDNITAVLLHFTGEHLPHPDGEPVTAPQSLERDRSLPRRVEIQYVSPQESESTLE
                     "
     gene            complement(2860..3600)
                     /locus_tag="PKZ53_04540"
     CDS             complement(2860..3600)
                     /locus_tag="PKZ53_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNJ39735.1"
                     /translation="MPNSYNYQTLPSNDNINPYSYCLEVRDAMFMRKGKMIAYYGNLR
                     FSALGDSMYAVIVDKVFNAPGYISDFVIVNGQGKVVLGDNGNNIASYNLESGNLTVKS
                     SHVLGFDSSLVCQESTQPGYLTLMGTGTFLASSNGPVHFIEPPCRVDEQALLGWADCP
                     SPSYHYDYAYIRNIFDVVGSMILGRTSGEEKQIDFFGQGTVLIQSSEEELAGRMDLQR
                     LTSEISGLGQHDLNQLSSFINNLMRPGE"
     gene            complement(3613..4251)
                     /locus_tag="PKZ53_04545"
     CDS             complement(3613..4251)
                     /locus_tag="PKZ53_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNJ39736.1"
                     /translation="MPTFEMINSKLLCVRLNNEEVFTKRGAMIAYKGNVNFAKSSGGA
                     GFGQAAMRAVTNENLKLMVSRGTGEVFYAYLGQHVTLIKLNGERFYAESESILAYDSR
                     LRAGTEFMGNQGGMSGLVRGAVTGQGLFTTTLEGHGELAVLSDGDMIGIDVNPSNPVF
                     VDPDAYIGHRGQLSSSIKTDVSWKTFVGQTSGESYQLQFTGNGTVYIQASER"
BASE COUNT         1059 a         1187 c         1093 g         1034 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac ccgattatgg
       61 aacaccttcc caaactggaa ctgatctctg aatatccagc gattccagcc aggtctgaca
      121 aaacctttta tgtgctggtc cagatcacac cgcctgaacg tgcgaaggaa tcgtaccgac
      181 caccgttgaa catcagcctc gtcctggatc ggtcagggag tatgggggtt gataagctca
      241 atcaggccaa gcaggccgcc tgctcgttca ttgatcggct gctgccaact gaccgcctga
      301 gcctggtgac gtttgacacg cgggttcagg ttctggtgcc gagcaccctg gtgaccgaca
      361 agcaggccct caaggccgcc gtggctgaaa tctacggcgg cagtcgcacg gcgctccacg
      421 aagcctgggt gcgtggtgga ctcgaagtca gtcatggcgc gacgtcgagt tcgatcaacc
      481 gggttattct tttaaccgat gggattgcca atgtcggaga aacctgtcct gaaacgattc
      541 tcaaccacac ctcagagttg acccagcgcg ggattacgac gtcaacgatt gggattgggt
      601 ttgactttca ggaagacctg ctgattccaa tggctgaggc cggcagcggc aatgcgtttc
      661 acgtggtgcg gacaactgat cttgaaccgg tgctgacggc tgaagttgac aacatgattt
      721 cccagattgg tcattccacc cggcttgggt ttataaccag ttctgctttg caggtgcgtg
      781 aaactctcaa tgacttcgca cctgttcaca aacaggaggt tcgacttcca aatttgcgtg
      841 ccggtcaccc gctccaaatc gtgctggcaa ttgctgccaa tgtgaaaacc agtacccggc
      901 cccaaacggt ctcaatccga ttgacctggc aggcccaggc gaccggagac cgtcatagcg
      961 tgaatcagac cttatttctt ccgattggaa tgacttcgga gaaatcagcc catccagagg
     1021 ttcggaaact ggtcggattg ctgacgattg cccggatcaa acgcgaggcc atgcggcaga
     1081 ttgatgccga acgctatgcc gaagcccggc aattgatttt cagcgccatc cagatgaccg
     1141 ccacgctttt gacaaatgtg ggtaatgatt ttcgattacg cgcacaactt aaggcacttg
     1201 atcaactgga acacgacctt gaacagggag atgacccggc aaaatcccgg aaatttctgg
     1261 cgtatcaaag ctatgccacc agtacctgcc gattggatag ccagccggac cgaaaaaccg
     1321 gaagttgaag gcggcagaca tcgaacaatt tgaccacctg attgatttca ggtgacttcc
     1381 ttcgagcact tttttgatga gttgaggcaa accaggcaaa cccaaacagg ttttgagcgt
     1441 acgatcaaaa cttaaatttc aaccaaatcc ttaaatatac attgaaatag cattcagtat
     1501 tttacactgc attcaagaac agttattttg acacaaagga gtaaaccacc ttgccgagca
     1561 gcacattgga gattccgatt atgacagacc gttcagttca cccaaccagt cagctcgcag
     1621 ttaaaatctc ggcgttgacc gacgttggtc gggttcgaag tggaaatgaa gataactttt
     1681 tagtgattga ccttgagacc ggatttacct ggactgcaga ttccgatcct cccgaaaata
     1741 atcctttcac cattacccca gcagacagcg gcctgttatg tgctgtttcg gatggaatgg
     1801 gcggcgcggc tgcgggtgaa gttgccagta agctggcggt tgcctggtca ggctggatta
     1861 tgtcggaagc ctgtgcccac cccgatctca aaatttatcc ctttcacgag cggctccgac
     1921 tcgccatcga acaagccaat attcaaatcg ccatggcggc tgaagaagcc ccggaatttt
     1981 ccggcatggg cgccaccttt accgcagccg ggattcaagg aaacagcctg accctggccc
     2041 aggtcggtga ctcacgcgcc tatctgatcc gcaatggcca tatggtccaa ctgacccgtg
     2101 atcaatcgct ggtcgcccag ttgattgaaa tggggtccat taccgaagaa gaagccgaat
     2161 ttcaccccta tcgcaatgtc atcatgcagg ccctcggcgc caatgcctgg gttgaagtcg
     2221 ccgtccaggt catcaacctg tgtcaaggcg atctggtcct gctatgcagc gatggactct
     2281 cgggcaaagt tcgcaaagac cagatgcaga agctcatcac cgaagtcagt catgatttac
     2341 ggcttggggt tcgcaaactc atctatgccg ccaacgaagc tggcggcgac gacaacatca
     2401 cggcagtgtt gctccacttt accggggaac acctgccaca tccggatggt gaaccagtca
     2461 cggcaccaca atcgctcgaa cgtgaccgct ctctgccccg gcgggttgag atccaatatg
     2521 tctcgccgca ggaaagcgaa tccacgttgg aataaggttc cgggttccga gttccgggtt
     2581 tttgaaaccc ggaagtgagc cgttctgatc actcatttct tgttttggtt tttcaacaaa
     2641 atggcctgcc agggtggcag gccgttttgt tttggggatt tgagctaaga ggcgtggggt
     2701 tataccattt tggaatggag tcctcgtatt actggaaagc taaattattg aaaaatttgt
     2761 atttccctga cctctggccc cgagcccctg atcccaaaat ggtattacct tacccatcga
     2821 aaacccggaa cccggaaccc ggaacccgga acccggctct tactctcccg gtcgcatcaa
     2881 gttattgata aagcttgaaa gctggttcag gtcatgctga ccgagacccg aaatctcgct
     2941 ggtcaagcgc tgtaaatcca tccgcccggc cagttcttct tcagacgatt gaatcaacac
     3001 cgttccctgg ccaaagaagt caatttgttt ttcttcgcct gaagtccggc ccagaatcat
     3061 tgaaccaact acgtcaaaga tattccggat gtaggcataa tcataatgat aggacggact
     3121 tggacagtcg gcccagccga gcagtgcctg ttcgtcaacc cggcagggtg gttcgataaa
     3181 gtgaaccggc ccgttggacg aggccagaaa ggtcccagtt cccatcagtg tcaaatagcc
     3241 cggctgggtt gattcctgac agaccaggct tgaatcaaat cccaggacgt ggctgctttt
     3301 caccgtcagg ttgccgcttt ccaggttgta tgaagcgata ttgttgccgt tgtcgcccaa
     3361 aacgactttc ccctggccgt tgacgattac aaagtcactg atgtagccgg gagcgttaaa
     3421 aaccttatca acaatcacgg catacattga atcaccgagc gccgaaaacc gaaggttgcc
     3481 gtaataggca atcattttgc ctttgcgcat aaacatggcg tcgcgcactt cgaggcaata
     3541 gctgtaaggg ttgatgttat cgttggatgg aagtgtttga tagttatagg aattgggcat
     3601 aggtccacct tcttacctct cgcttgcctg gatgtacacc gtgccgttgc cggtgaattg
     3661 caattgatag ctttcgccgg acgtctggcc gacaaaggtt ttccaactga cgtcggtttt
     3721 gatgctcgac gacaactgtc cgcgatggcc aatgtaggca tccggatcaa caaacaccgg
     3781 attggacgga ttgacatcaa ttccaatcat atcgccgtca gagaggaccg ccagttcacc
     3841 gtggccttcg agcgtcgtgg taaacaatcc ctgaccggtt accgcaccgc gaaccagccc
     3901 tgacattccg ccctgattgc ccataaactc tgtcccggcc cgcaaccgcg agtcataagc
     3961 cagaatactt tcgctttcag cataaaatcg ttcgccattg agcttgatca gcgttacgtg
     4021 ttggcccaga taggcgtaaa acacttctcc ggtgcctcgc gacaccatca atttcagatt
     4081 ttcattggtt acggctcgca tcgcggcctg accaaagccg gcaccgccgc ttgatttggc
     4141 aaagttgaca ttgcctttgt aggcgatcat ggccccgcgc ttggtaaaca cttcttcgtt
     4201 gttgagtctg acacacaaca gtttgctgtt gatcatttca aaagtcggca tgcgcttatc
     4261 tccttaccat acaaacaaat gaagaactac tttgaatgta gaattgcgaa ttcttcattc
     4321 ttatcccacc cgctgacgca ggtggtactg acttcttcat tccgttttat ttc
//
LOCUS       DAOQDO010000205         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13713_length_4361_cov_2.949141, whole genome shotgun sequence.
ACCESSION   DAOQDO010000205 DAOQDO010000000
VERSION     DAOQDO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13713_length_4361_cov_2.949141"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..2310
                     /locus_tag="PKZ53_04550"
     CDS             16..2310
                     /locus_tag="PKZ53_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNJ39737.1"
                     /translation="MIDLGDFADRFADSGQRVMKRAFEESKRRDHNFLAPEHIFLALT
                     EVEKNLFNDVMQSLNLDPQAVLNEFNGRLGHQRQYVGRRMKLHDSTRNLLSAAMMRAR
                     KGGRQTIESVDLFASMFEDQESASVSVLKQFGAEAQEVVEKITSRVKTREEKEAKISR
                     KFELPPYLKHFGVSLNKLARQDKLPPVIGREQEIRQVMEILCHRERANSPMLVGDPGV
                     GKTAVVEGLARMIELEPEKVPQRLRNSHVVHLQISGVVAGTMLRGMFEERIQGLINEV
                     KERENIILFIDEAHTLIGAGNALGASSDAANMFKSALGRGELRIIGATTITEYKEYIA
                     EDEALARRFRMVKINEPNLDDTRRILTGVRSRLERNYSVQITDEALETSLEMAPRYMR
                     NLHMPDKVIGWLDTASVKVEMDHPEHPVVTSADVIEVISQESQIPRDMIFRETNRRFG
                     DMEAGIGQRVIGQHEAIKAVARRLRLNKGPLKENFYKPDGVLLFLGPTGVGKTELAKA
                     VAEFMFGDEGKMIRIDMSEYQDGTVAVEKLIGMPRGIVGSERGGVLTEKLRDNPYTVL
                     LLDEVEKASPYLLNLFLQAFDEGWLTDGRGKKVYLSDAIIIMTSNLGSENFKKYMKPL
                     GFGTKTLGDMREIKNEVLKVAEQRFSPEFRNRIDEIVVFSPLTRDEVRQVCDLYLSKI
                     ENQMVRRGKTVEVTEDARNLLVEKGFSPAYGARFLKRTIDEKVKIPLTAQWDNGNIFV
                     VEAENGEIVVKNTDPAVLSKDSMN"
     gene            2413..2919
                     /gene="sixA"
                     /locus_tag="PKZ53_04555"
     CDS             2413..2919
                     /gene="sixA"
                     /locus_tag="PKZ53_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNJ39738.1"
                     /translation="MEIYLLRHGIAYDLGDGGSRTDAERALSPDGKIKMYQQAEGMRQ
                     LDLKFDAILTSPLIRARETADIVAEVLRQSEKLEMCQVLALGADPEKVCTLLRDQFAS
                     AGAVLLVGHEPDLSLLGAYLISSRGDAALQMKKGSLCRIDSLRVVKAGGSLRWLLTPT
                     QLRLLGKK"
     gene            complement(3040..3297)
                     /locus_tag="PKZ53_04560"
     CDS             complement(3040..3297)
                     /locus_tag="PKZ53_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39739.1"
                     /translation="MKLFAFFRGQGIHNARLVRVETINSLAQLSPLARTEMRERLRDL
                     VTKAEQKRRLRNLQPLSAALVSSITAGREQSDEGDSRCLAA"
     gene            3760..>4361
                     /locus_tag="PKZ53_04565"
     CDS             3760..>4361
                     /locus_tag="PKZ53_04565"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ39740.1"
                     /translation="MRRTGNLNDWNRSTGGQGGWRRPLTPGVKYLMIFTGVAYLGQVY
                     AVKQGLIDPWFLALVPERVIQGAVWQFITALVAYAISPDATSNLVSLIFGLLGFYFVG
                     TLLEGTWGTRRFLWYYVRAGVATHLIAFLFMALIGQVHTRFPVSSATAVVFGTFLGSF
                     GALFWEQQVNFNFVPLRGKTLVWIMTGVAIFMLLRGDFWWA"
BASE COUNT         1083 a          978 c         1220 g         1080 t
ORIGIN      
        1 cgaaagaggg tttttatgat tgatcttgga gattttgcag atagattcgc agacagcgga
       61 caacgagtaa tgaagcgagc ctttgaagaa tccaaacgcc gcgatcacaa cttcctggcg
      121 cctgagcata tttttctggc tttaaccgaa gttgagaaaa accttttcaa cgacgtgatg
      181 cagagcttaa atctcgatcc gcaggcggtt ctcaatgaat tcaacggtcg tttgggtcat
      241 caacgccagt atgtcggtcg gcggatgaag ctccatgatt caacccgtaa cctcctctct
      301 gctgcgatga tgcgtgcgcg aaaaggtggc cggcaaacca ttgaatcggt tgacctgttt
      361 gcctcgatgt ttgaagacca ggaaagcgcc tcggtgagtg tgctcaagca gtttggtgct
      421 gaggctcagg aagtcgtcga aaaaattacg tcacgagtaa aaacacgcga agaaaaagag
      481 gccaaaatca gccgcaagtt tgagttacca ccctacctga agcattttgg cgtcagcctc
      541 aacaagctgg cccgtcaaga taagttgccg ccagtgattg ggcgtgagca ggaaatccgt
      601 caggtaatgg aaatcctgtg tcatcgggaa cgagccaatt ccccgatgct ggtgggtgat
      661 cctggggttg gaaaaaccgc cgtggtcgaa ggtctggccc ggatgatcga actcgaacca
      721 gagaaggtcc cacaacgact gcgcaattcg cacgtggtcc acctccagat ttcgggggtg
      781 gtggcgggga ccatgttgcg cggtatgttt gaagagcgaa ttcaggggct gatcaatgaa
      841 gtcaaagagc gtgaaaacat cattctgttt attgacgaag cccacacttt aattggagcc
      901 ggaaatgccc tgggcgcctc gtcagatgcg gcaaatatgt ttaagtcggc cctcgggcgc
      961 ggtgaattgc ggatcattgg ggccaccacg attactgaat acaaagaata tattgctgaa
     1021 gatgaagctc tggcacgacg cttccgaatg gtgaaaatca atgaacccaa tctggatgat
     1081 acccggcgaa tcctgaccgg tgtccgctca cgactggagc gcaattactc agttcaaatt
     1141 actgacgaag cgcttgaaac ttcgcttgaa atggcgccgc gctatatgcg aaatctacat
     1201 atgcccgata aggtgattgg gtggctggat actgcatcgg tgaaagtcga aatggatcat
     1261 cccgaacatc cggtcgtgac atcggcggat gtcatcgaag ttatttcgca ggaatcgcaa
     1321 attccacgcg atatgatctt ccgagaaacc aatcggcggt ttggcgatat ggaagccgga
     1381 attggccagc gagtgattgg tcaacacgaa gccatcaaag cggttgcccg gcgattgcgc
     1441 ctcaacaaag gtccattgaa agaaaatttc tacaaacccg atggtgtttt gctcttcctt
     1501 ggtcccaccg gggtcggtaa aaccgaactg gccaaagccg tggctgaatt catgtttggt
     1561 gacgaaggca agatgattcg cattgacatg tctgaatacc aggacggcac ggttgcagtt
     1621 gaaaagctga ttggtatgcc gcgcggcatt gtcggatccg aacgcggcgg ggttttaacc
     1681 gagaagcttc gcgataaccc ctacactgtc ttactgcttg atgaagttga aaaagcctcc
     1741 ccgtatttgc tcaatctttt cctgcaggca tttgatgaag gctggttaac cgatggccgt
     1801 ggcaaaaagg tgtatctttc agatgccatt atcattatga cctcgaatct gggcagcgaa
     1861 aactttaaga agtatatgaa gccgcttgga tttggcacca aaaccctcgg cgatatgcga
     1921 gagatcaaga atgaggtcct caaggttgct gaacagcgat tttcccccga atttcgcaat
     1981 cggattgacg aaattgtggt gttttcaccg ctgacccgtg atgaagttcg ccaggtttgc
     2041 gacctgtatc tgtccaaaat cgaaaatcag atggttcgtc gtgggaaaac cgtggaagta
     2101 accgaagatg ctcgcaactt actggttgag aaagggttta gcccggcata tggtgcgcgg
     2161 ttcttgaaac ggaccattga tgaaaaggta aaaatcccat tgactgccca gtgggataac
     2221 ggaaatatct tcgttgttga ggctgaaaat ggcgaaattg tagtgaaaaa caccgatcca
     2281 gccgtgcttt caaaagattc aatgaactga attgagtgat caagcggttc agtggtacat
     2341 ataaaggcga ggttgcctga cctcgccttt gttgtttttg tggaaaaaca ctcatcacgg
     2401 aggacttgag acatggagat ttacctgctc cgccacggca ttgcctatga tttaggtgat
     2461 ggaggatcgc gaacagatgc agaacgggca ctcagcccgg atgggaaaat caaaatgtac
     2521 cagcaggctg agggtatgcg tcagcttgat ctgaaatttg acgcaattct caccagcccc
     2581 ctgattcggg cacgtgaaac ggctgatatt gtggctgaag tattacggca gtcagaaaaa
     2641 cttgaaatgt gccaggtttt ggcgcttggg gcggatccag aaaaggtatg taccctctta
     2701 cgagatcagt ttgcgtcggc tggtgctgtg ttgctggttg gacatgaacc ggatttgagt
     2761 ctcttggggg cgtatctgat tagttcacgt ggggatgcgg cgttgcagat gaagaaggga
     2821 agtttatgtc ggattgatag tctgcgcgtg gtgaaagctg gtggaagttt acggtggtta
     2881 ttgacgccaa ctcagttgcg attgctgggg aaaaagtagg agtgatcgga ggtgtagggg
     2941 aaagcggaga gagatggaat gggggtggag ttttcaggaa gtgattcgag aacgaggaag
     3001 aagtgtatcc attcacgttc ccgaatcact tgcctatccc tatgctgcta gacatctact
     3061 gtccccctcg tctgattgct cacgtccagc ggtgattgag ctcaccagtg ctgccgagag
     3121 tggctgcagg ttgcgcaatc gtcgcttttg ctcagccttg gtgaccaggt cacgcaaccg
     3181 ttcacgcatt tcagtgcgtg ccagcggcga aagctgggcc agggaattga tggtttcgac
     3241 acggaccaaa cgggcattat gaatgccctg accacgaaaa aaggcaaaga gcttcattgg
     3301 atgtatccct cctttgatta ttttttacaa cgatcaaaaa gcgaaagggc caacccgctt
     3361 ctatacagca ggttggccct cacgaggtga agcccgtttg tctgaacgtg gtctactgac
     3421 cgcctccaga tgtgctgctg tacagatggg tacctggata caacacacag ccgttctgag
     3481 ccaatgaaca gaacgtgcta ttcgcaaaat acagcacaac caacgatgaa aactgccgag
     3541 agtttccagt tggttcaggt atgttgctac tactgcttgc tgacatagtg cctccaggca
     3601 acctttctaa aaaatgtatg gaacaggcca ttatccacga aacagggagc cggtcaagtc
     3661 cttttcctgg tttagacgca aaattttcag cagaggtttg ccaaaagatg cgggtgcgtg
     3721 agatagtgct ctagcaagac ttcggcaaag gagttatgta tgcggcgaac gggcaacctc
     3781 aatgattgga atcgctcaac tggtggtcag ggaggatggc gacgaccgct gacacctggc
     3841 gtgaaatatt tgatgatctt cacgggggtg gcttatcttg gacaggtcta tgctgtgaaa
     3901 cagggtctga ttgatccctg gtttctggca ctggtccctg agcgtgtgat tcagggggcg
     3961 gtctggcagt tcatcaccgc cctggtggcc tatgcaatct ctcctgatgc caccagcaac
     4021 ctggtgagtc tcatttttgg gttgcttggg ttttattttg ttggaacctt actggaagga
     4081 acgtggggca cccgccgctt tctctggtac tacgtacggg ccggtgtggc cacacacctg
     4141 attgcttttc tcttcatggc attgattggc caggttcata cccggtttcc tgtttcaagc
     4201 gccacagccg tggtgtttgg aacgttcctt ggaagttttg gggctctatt ttgggagcaa
     4261 caggtgaatt tcaactttgt tcctctccgt gggaaaaccc tggtctggat tatgaccgga
     4321 gtcgcgatct ttatgctgtt gcgtggtgat ttctggtggg c
//
LOCUS       DAOQDO010000206         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13769_length_4348_cov_3.281621, whole genome shotgun sequence.
ACCESSION   DAOQDO010000206 DAOQDO010000000
VERSION     DAOQDO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13769_length_4348_cov_3.281621"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKZ53_04570"
     CDS             complement(<1..335)
                     /locus_tag="PKZ53_04570"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNJ39741.1"
                     /translation="MLAHALVTTQAPPQFHRIIGTYGGTAPGPQVICIGGIHGNEPAG
                     VHALKRVIHQLNETRPPFQGEIVALSGNLNALNSECRFFSKDLNRQWTTAKIQAVKDT
                     LRPDELDAE"
     gene            complement(355..642)
                     /locus_tag="PKZ53_04575"
     CDS             complement(355..642)
                     /locus_tag="PKZ53_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39742.1"
                     /translation="MHPYKDCHDCNNAFYEPTQPNGELACLKCGELILASESEPEMTM
                     THAYWETVRENMLTSGQLAIDHVRQGDAEVARFFAVEAAHDARHLEKFNRL"
     gene            1202..2122
                     /locus_tag="PKZ53_04580"
     CDS             1202..2122
                     /locus_tag="PKZ53_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ39743.1"
                     /translation="MKVLMATDGSLDALTAMRAAAQLVRKENSQVQVVCVIPELFQAK
                     SQTREAGEQSRRARDRYRRQISRETRKILDEATRILAEEGIAAESVMEFGSPANVIVH
                     KAAAQDLTVIGALGKYDRTRLGLGPVANRVIESAPGMILVGRELTPDKSLRILVGVDG
                     SEASEQALRRLVTEFNVNSAEITLMHVVETPWIKLGLDRDFLDAPDEWSDSGDQPLHL
                     ERELRAEAEMLVDEARRILQPYQVSIDTVIEEGNAATELLGQVENGDYDLIVLGATGL
                     TDLRHGLVGSVSSKVAWTASCSVLIVKTSA"
     gene            complement(2214..2381)
                     /locus_tag="PKZ53_04585"
     CDS             complement(2214..2381)
                     /locus_tag="PKZ53_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39744.1"
                     /translation="MSSEAIDQRLRDVAQLFRLGMTIQNIRVLGNAEELLAPPCSDVL
                     DLPNSSPFDPE"
     gene            complement(2407..2877)
                     /locus_tag="PKZ53_04590"
     CDS             complement(2407..2877)
                     /locus_tag="PKZ53_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39745.1"
                     /translation="MDLYEEFVALIDILNETGINYALCGGLAVAFFGYVRFTKDIDLL
                     IIGEDLPRIRKALEPRGFVFYSGATPFDPGSPTSRTIHRLSKIEGSDVLPIDLIIATP
                     LFDQVWKQRQVVEWNGRSVQIVSREGLRFMKQLADRNQDRLDLAQLGLGENKDV"
     gene            complement(2927..4270)
                     /locus_tag="PKZ53_04595"
     CDS             complement(2927..4270)
                     /locus_tag="PKZ53_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ39746.1"
                     /translation="MLSPDQLKVCVIGAGPSGITAAKNLRQVGITNLVVYEKSHQVGG
                     NWAFSLQQSHSSVFETTHIISSKTLSQYLDFPFPQEYPDYPSHKQLLEYFQSYAREFD
                     LLRFIRFQTEVTAVRPAPNHRWQVTVNDGTTDTFDAVLVSNGHHWNPRMPTYPGTFTG
                     QLLHSHDFKTNQPFRDQRVLVIGGGNSACDIAVETSRISQRTVLSMRRGYYFIPKFMF
                     GLPSDVVNAKLQFLPKSLRTHLLKIGLDFSIGKPELYGLQKPDHQLLQTHPVVNSELL
                     YFIRHGKIHPRPDIARFEGQNVVFVDGQSEEIDVIIAATGFHITFPFFEPGFVDFDGT
                     EVPLYLKVFHADHPTLFFIGLIQPMGCIWPLADLQSKLVANTLIGNWHPPANIRELID
                     WEVNHPAYDFVKTPRHSVEVDYHLYSKALLRHIPKDAPDWKDQFQNGQPVGTHLR"
BASE COUNT         1120 a         1012 c         1142 g         1074 t
ORIGIN      
        1 tcttccgcat ccaattcatc tggccgaaga gtgtctttga ctgcctgaat tttggcggtt
       61 gtccactgtc ggttgagatc tttgctaaaa aaccgacact cagaatttaa ggcattgaga
      121 tttccgctga gcgcgacgat ttcaccttga aatggcggtc gggtttcatt gagctggtgg
      181 atgacgcgtt ttaaggcgtg aactccggct ggttcgtttc catgaatgcc accaatgcaa
      241 atcacctgcg gccctggtgc ggttccacca taggtgccga tgattcggtg aaattggggg
      301 ggagcttgag tggtgacaag tgcgtgggct aacacaacaa atacctcgtt ttctttacaa
      361 gcggttgaat ttttcaagat gccgggcgtc gtgggcggct tcaaccgcaa aaaaacgcgc
      421 cacttcggca tcaccttgcc gaacatgatc aatggccagt tgaccactgg tcagcatgtt
      481 ttccctcacc gtttcccagt aggcgtgagt catagtcatt tcaggctcgg actctgaagc
      541 caaaatcaac tcgccacact tgagacaggc taattcgcca tttggttgag tcggctcata
      601 aaacgcattg ttgcaatcat gacaatcttt atagggatgc atagtcacac tcctgagaaa
      661 aagaaggatc gaatatagaa ataaaataga tttgaacctc atttcaactg gagcaacttg
      721 tgttccagta atgccaggtt atggctggtt ggcactggcg gtgagaaaac accatgccct
      781 ttttggtttc aagccgggcc attgaaggaa gaaatcctgt atcatatacg aaaaccagag
      841 gttaactggc ctccatgtca gtttgggtat ttttttcgag tacgggctgg gtaccacctc
      901 gtcagccaat ccgacggctc cttcgcaaaa aacgcgattt ggcaaagggt ggattaagtt
      961 tgaggtgggg attatttcga ttgtatttct gggtgatgag atttgagcgc tgttcgatta
     1021 aatggactgg ggagatttcc acactccaat gggagaatct gcgcatcaat cacagcaaat
     1081 cctggcattc agcggcaaaa gagggttggt atggaaattg attgatctca aaagcatcag
     1141 tgatgttctt taatgaaaag gactggccta ctcatcccga ctctttcaga agtgaggaaa
     1201 tatgaaagtt ctcatggcga ccgatggatc actcgacgca ctcaccgcaa tgcgggctgc
     1261 cgcccagtta gtgcgcaaag aaaacagtca ggttcaggta gtatgtgtta tccctgaatt
     1321 gttccaggca aaatcgcaga ctcgtgaagc tggcgagcaa tcgcggcgag cacgtgatcg
     1381 ctaccgccga caaatcagcc gtgaaacacg aaaaatactc gatgaggcta cccgcatcct
     1441 ggctgaagag ggaatcgcgg ctgagagtgt gatggaattt ggttcgccag ccaatgtgat
     1501 tgttcacaaa gccgccgccc aggacctgac cgtgattggg gcgctcggaa aatatgaccg
     1561 aacccgactt ggactgggcc cggtggcgaa tcgagtgatt gaatcggcgc caggaatgat
     1621 tctggttggc agggaattga cgcctgacaa atcactccga attctggtcg gagttgacgg
     1681 ctcagaggct tcagagcagg cgctccgccg actggtcacc gaatttaatg tgaattcagc
     1741 cgaaataacc ctcatgcacg tggtggaaac cccctggatt aaactgggcc tggatcgtga
     1801 ctttctggat gcccctgatg agtggagcga ttcgggagat cagccattgc atctggagcg
     1861 tgaacttcgt gccgaagccg agatgctggt tgatgaggcc cgacggattt tacaacccta
     1921 ccaggtctca attgatactg tgattgaaga agggaacgcg gcaaccgaac ttctgggtca
     1981 ggtcgagaat ggagactatg atttgatcgt ccttggtgcc accgggttga ctgatttacg
     2041 acacgggctg gtcggcagcg tttcaagcaa agttgcctgg acggcctcct gttcggtgct
     2101 gattgtgaaa acatcagctt gaattgaaag ctggttaatc ctgtgagatg gaattgggcc
     2161 ggctcaacgc agattgagac gggttctctt ccacaggaag cgaaatccct ggtttattct
     2221 ggatcgaaag gggatgagtt gggcaaatca agaacatctg aacacggtgg agccaggagt
     2281 tcttcagcat ttcctaaaac ccggatgttc tgaatcgtca tacccaggcg aaaaagctgg
     2341 gcgacatccc ggaggcgctg gtcaattgct tctgaagaca tatcaattgg gttcgtttgc
     2401 aaatggtcag acatctttgt tttcccccag gccaagttgg gccaaatcca gccgatcctg
     2461 gtttcgatcc gcaagctgtt tcatgaaccg aagtccctca cgtgacacaa tttggacgct
     2521 tcgaccattc cattcaacaa cctggcgttg cttccacacc tggtcaaaga ggggagttgc
     2581 aatgatcaaa tcaattggca gcacatcgga gccctcaatt tttgagagtc ggtggatcgt
     2641 ccgtgatgtt ggactccctg gatcaaatgg agtggcccca ctatagaaaa caaacccgcg
     2701 tggttccagt gcttttcgaa tccgcggtaa gtcttcacca atgatcaaaa ggtcaatgtc
     2761 cttggtaaat cttacgtatc caaaaaaagc aactgccaga ccaccacaga gtgcatagtt
     2821 gatcccagtc tcattcaata tatcaatcag tgcgacaaac tcttcatata aatccatgtg
     2881 ttattttctt cctcagactt gaagcggtat ttggaaagct cctggcttac ctcaagtgag
     2941 taccaactgg ctggccgttc tggaactggt ctttccagtc aggagcatcc ttcggaatgt
     3001 gccggagcag cgctttacta tagaggtgat agtcaacttc aaccgagtga cgtggcgttt
     3061 tgacaaaatc ataggccggg tggttgacct cccagtcaat caattcccga atattggccg
     3121 gcgggtgcca gttgccaatc agtgtattgg ctaccagttt ggattgcagg tcagccagtg
     3181 gccagataca gcccatcggc tggatcaacc caatgaaaaa caacgttggg tggtcggcat
     3241 gaaagacctt taagtacagc ggaacctctg tgccgtcaaa atcaacaaag ccaggctcaa
     3301 aaaacggaaa ggtgatatga aacccggtgg ccgcaataat gacgtcaatt tcttcagact
     3361 gaccatcaac aaagacaacg ttctggccct caaaccgggc gatgtcgggt cgaggatgaa
     3421 ttttgccgtg gcgtataaag tacaaaagct cggaattgac caccgggtgg gtctgcaata
     3481 attgatggtc tggtttttga agcccgtaca gttccggctt cccaatcgaa aagtccaacc
     3541 cgattttcag caggtgagtt cgcaacgatt ttggcaggaa ttgtaatttg gcattgacga
     3601 catctgatgg caggccaaac ataaacttcg gaataaagta gtagccacgc cgcatactga
     3661 gaacggttcg ttgggatatc cgactggttt caaccgcgat atcacaggct gaattcccgc
     3721 caccaatcac cagcactcgc tggtcacgaa acggttgatt ggtcttaaaa tcatgcgaat
     3781 gaagcagttg accagtaaag gtgcctgggt aagtgggcat ccgcgggttc cagtgatgtc
     3841 cgttacttac caaaacagcg tcaaaggtat cggttgtccc atcgttaacc gttacctgcc
     3901 agcggtgatt gggtgctggc cgaacggcgg tgacctccgt ttgaaaccgg ataaaacgaa
     3961 gcaggtcaaa ctctcgggcg tagctctgga aatactccag caactgtttg tgagatggat
     4021 aatcagggta ctcttggggg aatggaaaat ccagatactg ggaaagcgtc tttgaactga
     4081 tgatatgggt ggtttcaaaa acgctggaat ggctttgctg aagtgaaaat gcccagttcc
     4141 caccaacctg atgacttttt tcgtagacga ccaggtttgt gatgccgacc tggcggaggt
     4201 tcttcgccgc tgtgattcca gatggaccag caccaatcac gcacaccttg agttgatcag
     4261 gggaaagcat agtggtattc ctcagagagt ttcagtcaag ttcggtactt cgtttttagc
     4321 attttctatt cctgagagaa aaggattc
//
LOCUS       DAOQDO010000207         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13855_length_4330_cov_2.784094, whole genome shotgun sequence.
ACCESSION   DAOQDO010000207 DAOQDO010000000
VERSION     DAOQDO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13855_length_4330_cov_2.784094"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKZ53_04600"
     CDS             complement(<1..575)
                     /locus_tag="PKZ53_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ39747.1"
                     /translation="MKPPLPMIDGVSPSSKWLPVGHWKTLFDFLQDQFPEVEPATWLS
                     RMAKGQVVDETGRCLDAKSPYRAGACIFYYRELESEPCIPLAESVVFQDEHLLVADKP
                     HFLPVVPAGRFLQETLLVRLKKKLNLESLVPLHRIDRETAGLVLFSINPATRNQYTRL
                     FQNRTIEKVYHALAPQPSHLSFPLVRQSRIVP"
     gene            complement(593..1447)
                     /locus_tag="PKZ53_04605"
     CDS             complement(593..1447)
                     /locus_tag="PKZ53_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ39748.1"
                     /translation="MTVSLDSHITSATEQLAPEAVFEPSASTSLFTSTTIRTMIVSTD
                     TLIRNQLRNWLEDRPEFECVGECATGYGTLKALEEGDVELLFLDHELPWMDGFQVAET
                     IRRSPLPGIVLLSSSELFAFRAFEIQAVDFLLKHGDRSRFDRALRRAKELIQTIRERD
                     VNQQLFSLLEKLKTPEPKYLEQVVVKTTRNSFLLKLNDVNWIEAEGNYVRLHLGRESH
                     LIRESISHFESKIDPKIFTRIHRSVIVNFERVAKWYPLFHGDYKLILQDGTELTLSRR
                     YRKNMKTI"
     gene            1778..4189
                     /locus_tag="PKZ53_04610"
     CDS             1778..4189
                     /locus_tag="PKZ53_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ39749.1"
                     /translation="MSKRIKHFYDFGLYRIDVGERFLYRAGELVPLSPKLFDTLLVLV
                     EHSGRVLEKDELMQLVWPETAVEESNLTQNISQLRKLLGEGHPEARLIETIPRRGYRF
                     VAEVTEVREGGDLDPWTSKSSLASVSLTSGGIVVPIPVKDRSRQLAAQPVLQLVESPA
                     VLPSPDIDSIGTSTIDQVEVESQVAGVSVDPPAARTEISRWAIGLLISMVLVAVLGGA
                     WWVYRQQAAKKSDPDVHTVEVVRLTSNGQVQFSAISPDGRYLTYSIDDHGLQSLWIQN
                     IASPQSIVIVPPAKVRYVGLTFSTDGNFVYYTQHKVPEEVTEHSWLPISSAQTDQGAL
                     FEIPVLGGTPRLIVDRISSRVGFSPDGRHMAFIRSQPESKATSIVIAQADGQNARSVL
                     TRQENEFVAYAAPAWSPDGTSLAFVVGGYEAAGFFTQLMVMNIKTGETQDATLREWGN
                     VEQAVWNQDGHSLLLTGTARHSSQSDQLWQLSWPEKKLRQITTDFSIYQSLSLATETP
                     ALVMTQTSRVCTLSLYASSDLNQSERQLFSGFMPPVSEHIGLSWTPDGKILYGSHQSG
                     NFDIWKMNADGTGQVQLTVNPEHDLEPVMSRDSHWIVFQSKRSGPSNIWRMEASGSNL
                     QQLTHGNGEYAPNFSPDGRWVVYFTFLNGKPAIGKVSIDGGEPVFLAVGKATRPIISP
                     DGKWIACYYRESLDQPLRLAILDFEQGIPVKIFQDVSLPDPAKIQWSADSKSLFFGRT
                     ERGITNLWKLPIESSLPQPVTKFKNEMIYRFSWSPDEKWLALERGTTISDVVLMRNFS
                     INKGF"
BASE COUNT         1048 a         1027 c         1148 g         1107 t
ORIGIN      
        1 gggacgatcc ggctctgtcg aaccagggga aatgacaggt gtgaaggctg tggcgccaga
       61 gcgtggtaaa ccttttcgat agttcggttt tgaaacagtc tcgtgtattg attccgggtt
      121 gctggattga tcgaaaacag aacgagtccc gccgtttcgc gatcaatccg atggagcggc
      181 accagcgact ccagattcaa ctttttcttg agtcggacaa gcaatgtttc ctgcaaaaat
      241 cgcccggctg gaacgacggg caaaaaatgc ggtttatctg ccaccagaag gtgttcgtcc
      301 tgaaacacga ctgattctgc caaaggaatg cagggttcag attcaagttc ccggtagtaa
      361 aaaatgcagg cgccggctcg atacggactt ttcgcatcca ggcaacgccc ggtttcatcc
      421 acgacctgtc cttttgccat ccgtgaaagc cacgtcgctg gctcaacttc aggaaattga
      481 tcctgcaaaa aatcaaagag tgttttccag tgaccgactg gcagccattt tgaacttgga
      541 ctgacgccgt caatcatcgg aagtggtggt ttcaaggctg gtccccccat ttctagatcg
      601 tcttcatatt ttttcggtag cggcgactga gcgtcagttc agtgccatcc tggaggatga
      661 gcttataatc gccgtgaaac agaggatacc atttggcgac gcgttcgaag tttacaatca
      721 ctgatcggtg aatccgggtg aatattttgg ggtcaatttt tgattcaaag tggctgatac
      781 tttcgcggat caaatgtgat tcacgaccaa gatgcaaccg gacatagttt ccttcagctt
      841 cgatccagtt gacatcattc agtttcagaa gaaagctatt ccgggttgtt ttgaccacaa
      901 cctgttccag atatttgggt tctggcgttt tgagtttttc aagtaacgaa aagagttgct
      961 ggtttacgtc gcgttcacgg atggtttgga tcaattcttt tgcccgtcgc agggcacgat
     1021 caaagcggct tcgatcaccg tgtttgagca gaaaatccac cgcctggatt tcaaaggcgc
     1081 gaaaggcgaa gagttctgaa ctggacaata gcacgatgcc tggcaatggt gaccggcgaa
     1141 tcgtttcagc cacctgaaag ccatccatcc aggggagttc atggtcaaga aagagtaact
     1201 ccacgtcacc ttcttcgagt gctttgagtg tgccgtaccc ggtggcgcat tcccccacac
     1261 attcaaattc gggacggtct tcgagccagt ttcgcaattg gttgcgaatc agtgtatcgg
     1321 tgctgacaat catggtgcga atcgtggttg aggtgaaaag tgaggtgctg gcggatggtt
     1381 caaacactgc ttcaggagcg agttgttcgg tggcgcttgt gatatgagaa tccagactga
     1441 ctgtcattgt tattactcca caggcattgg gactgaaaaa cctggggctg aagaaaccgg
     1501 gctgaagact cgcaagctca gggcaatcaa agggatgaag gatgacggat gaaggatgaa
     1561 agaaaatcag ttcttccacc cggttttttc agcccggttt cttcagccct gagcccgact
     1621 gcttccaccc ggtttcttca gcccaaggtt ttcagcctga cttgttttgt gaggtgaggt
     1681 tctttaggat tagctcatat cagaccaatc caatttctgt cttgagccgg ttctcaaaat
     1741 cttctcaaaa agttctcaaa aagttctgaa aagatgtatg tctaagcgta tcaaacactt
     1801 ctatgacttc ggcctgtacc gcatagatgt tggtgagcgc tttctgtacc gtgctggcga
     1861 actggttccg ctctctccga agctgtttga caccttgctg gtgctcgttg aacacagcgg
     1921 gcgggtgctt gaaaaagacg aattgatgca actggtctgg ccggaaactg cggttgaaga
     1981 aagcaatctg acccaaaata tttcccagct tcgcaagctc ctgggtgagg gccaccccga
     2041 agctcgtttg attgaaacca ttccccggcg tgggtatcgc tttgtggctg aggtgaccga
     2101 agttcgtgaa gggggagacc ttgacccctg gacctcaaaa agtagcctgg cgtccgtgag
     2161 cctgacgtct ggtggtattg tggtgccgat tccggtcaaa gaccgcagcc gacagcttgc
     2221 cgcacagcca gttctgcaac tggttgaatc acctgcggtt cttccatctc cagatattga
     2281 ttcaattggt acttcaacca ttgatcaggt tgaggttgag tcacaggtgg ctggagtttc
     2341 agttgatcct cctgccgccc ggaccgaaat ctcgcggtgg gccatcgggt tactgatttc
     2401 gatggtgttg gttgccgtcc tgggcggggc ctggtgggta taccgtcagc aagctgcgaa
     2461 aaaaagtgac cctgatgtgc atacggttga agtcgttcgg ctgacatcca atggacaggt
     2521 gcaattttct gccatatccc ctgatggccg gtaccttacc tactcaattg atgatcatgg
     2581 gctgcaaagc ctctggatac agaatattgc cagccctcaa agtattgtga ttgttcctcc
     2641 ggcaaaggtg cgatatgtcg ggcttacgtt ttccacagat ggcaattttg tgtattacac
     2701 ccaacataaa gttcctgagg aagtcaccga gcatagctgg ctgccgattt ccagcgccca
     2761 aaccgatcag ggagcgctgt ttgaaattcc agtgttgggc ggaacgccga ggcttattgt
     2821 tgatcggata agcagccggg ttgggttttc acctgatggt cggcacatgg cgtttattcg
     2881 aagccagccg gaatcgaaag ccacgtcaat cgtgattgct caggctgatg gtcagaacgc
     2941 gcgctcagtt ctgactcgtc aggagaatga atttgttgct tacgcggcgc ctgcctggtc
     3001 gccagatgga acatcactgg catttgtggt tggcggctat gaagccgccg ggttcttcac
     3061 tcagttgatg gtaatgaaca ttaaaactgg cgaaacccag gacgctacat tgcgtgaatg
     3121 ggggaatgtg gaacaggcgg tctggaatca ggatggtcac tcacttctgc tcactggaac
     3181 cgcccggcat agttctcaga gtgaccaact ctggcaactt tcctggcctg aaaaaaaact
     3241 gcgacaaatt accactgatt ttagcattta tcagagtctc agtctggcaa ccgaaacacc
     3301 agcactggtt atgacgcaga cttcacgggt gtgcactctg agcctgtacg catcgtcaga
     3361 tctcaatcaa tcagaaaggc aacttttttc agggtttatg ccgcctgtca gcgaacacat
     3421 tggcttgagc tggacaccgg atggaaaaat tctgtatggc tcgcatcaaa gtgggaattt
     3481 tgatatctgg aaaatgaatg cagatgggac cggacaggtt cagttgactg tcaatcctga
     3541 gcacgatttg gaacctgtga tgagtcggga tagtcactgg attgtctttc aatcaaaacg
     3601 gtcagggccg tcaaacatct ggcgaatgga agccagcggc tcaaatctac agcaactgac
     3661 gcatggcaat ggggaatatg ccccaaattt ctcgcctgat ggccgctggg tggtgtattt
     3721 caccttttta aatggcaagc ccgcaattgg gaaagtctcg attgacggag gtgaacccgt
     3781 gtttctggcg gtcggaaaag caactcgccc aatcatttca cccgatggaa aatggattgc
     3841 ctgttattat cgggaatcac tggatcagcc acttcgactg gcgattctgg attttgaaca
     3901 gggtatcccg gtgaagatat ttcaggatgt gtcccttcct gacccggcaa aaatccagtg
     3961 gagcgcagat agtaagtcac ttttctttgg ccgcactgaa cgtggtataa ccaacctgtg
     4021 gaagctcccg atagagagtt cgcttccgca acccgtgacg aagttcaaaa atgaaatgat
     4081 ctaccggttt agctggtcac cggacgaaaa atggctggct cttgagcggg gcaccaccat
     4141 cagtgatgtg gtcctgatgc gcaacttttc aatcaacaaa ggattttgaa cccaatgaat
     4201 ttgaaagaat tgattcctct cggaacgacg gcaaccaaat ctacccgcgt gacccgtgat
     4261 ttaaccgtgg cccattttca cgatgggatg cctgaagtat atggcacacc aatgatgatt
     4321 tacctgatgg
//
LOCUS       DAOQDO010000208         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13882_length_4323_cov_3.188613, whole genome shotgun sequence.
ACCESSION   DAOQDO010000208 DAOQDO010000000
VERSION     DAOQDO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13882_length_4323_cov_3.188613"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PKZ53_04615"
     CDS             <1..1396
                     /locus_tag="PKZ53_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ39750.1"
                     /translation="QFYLGCLAHASYLIGSEGEAAVVDPQRDVEMYLSEAQAQGLALK
                     YIIETHLHADFVSGHRELAARTGATIVFGHKANALFPHRAVVDGDELQVGAVTLKILE
                     TPGHTPEGISIVVSDTSEAHAPAKVLTGDTLFIGDVGRPDLVGSKGFSSEEMAGMLYD
                     SLHQKLLTLDDSVEVYPAHGAGSLCGKNLSTETSSTIGDQRRLNYALKPMSRAAFVAM
                     MTADLPEVPAYFPLDAEINRTGASPLVELGAARALTPEEGNKLIQQGYLVLDIRPAPQ
                     FGSGYIPGSLNIGLKGQFASWAGSLIPLTTPLLLVVESPDQVTEAVTRLARVGIETVG
                     GYLAGGIDAWKAAGLELKTVPQLSVHALASRLEAEPDLQVVDVRRPLEFCQGHVPTAL
                     NFQLAQLEKNLDHLDPARPTAVICAGGYRSSAGASLLSRHGFHQVLNIVGGTAAWIQA
                     GLKVEAEIESAACS"
     gene            1595..2515
                     /locus_tag="PKZ53_04620"
     CDS             1595..2515
                     /locus_tag="PKZ53_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ39751.1"
                     /translation="MPVFELNQSDTSVPVLEFRHVALSFGDTEVLRDLSFKLHQNELI
                     CLTGTAGTGKTVTLMLAMGLLQPDEGQILIYGKEIEHLSEEELLALRSRHLGITFQED
                     ALFTSLSVYENAAYRLVEQGWSEGAIRTAVSEIIAMVGLTDAMWKTPEELSGGMKRRL
                     EIARALVGWPSIMLFDEPTAGLDPVNAKNILDLILQARDLHQTSAIFVSKELHIIPYL
                     ATHRAQHDPEQGEVQIVRARHDNLPNTHVLVLSEGVVSFWGTVEEFSASHAASVLPLT
                     HPEQIPHEPEIYIPITTQSVRKQTRDSENS"
     gene            2545..>4323
                     /locus_tag="PKZ53_04625"
     CDS             2545..>4323
                     /locus_tag="PKZ53_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ39752.1"
                     /translation="MKPYSNSSETDLDRLTAELERLREENKRLRAENSKLQYRETLLT
                     QKARSLNLITKATRVGIWTWNQKTDEVHWSDECYQLMGLEPAETELTISLWRKCIHTE
                     DHSQLTEVADSSLEGHSFGDYEYRVVWPDGTVRWISEKARLFRDLDGMPGEILGGVMI
                     DVTDRKLAEMALQESESRYRTISEITSDYTYSFRIKPDKSFELEWVTDAIYRIFGYPK
                     DVISQPENWWQLVHPDDRVLFQQTGESIWAGRPIVRELRMVTIHGQIRWVRDHVRPIL
                     DQNQQLIRIIGATQDVTEQKYAIEALRRSEAQIRQIIDVIPGAVFQLQETDDHSPVVS
                     FVSARAYDLLGWSTQEIRLLTTQGNVLIHPDDQPQAYRSYLEARQTRTTLDLEFRVLT
                     QSQEYKWIRTCAVPLKKSPHVVEWVGISFDISRQKENEEDLRYHRELFETIYTNSTDA
                     IFLVDTQTDFIIDCNQRGVELFEYPEKNKLIGLRGGAFHKVPLSEAESRKFGTEIQSG
                     RTVTAEFEYVTQSGRSFWGSLASKMISVGGKRLNLVRITDITSRKQMEEEQYKIAKLE
                     SLGVLAGGIAHDFNNLLTAIIGNVELV"
BASE COUNT         1082 a         1031 c         1148 g         1062 t
ORIGIN      
        1 acagttttat ttagggtgtc tggcacacgc ctcgtatttg attggatccg aaggggaagc
       61 ggcagtggtt gacccacagc gtgatgttga gatgtatctc tctgaagcgc aggctcaggg
      121 gctggcgctg aaatatatta tcgagacaca tttacacgct gattttgtca gtggacatcg
      181 ggaacttgcg gcccggaccg gcgccacgat tgtgtttggg cacaaagcca atgccttgtt
      241 tcctcatcgg gcggtagtgg atggtgatga actgcaggtg ggagcggtca ctctgaaaat
      301 cttagaaaca ccaggtcaca cgccggaagg gatttcaatt gtcgtcagcg acaccagcga
      361 ggctcacgcc ccggcaaaag ttttgacggg tgatacactc tttattggtg atgttggaag
      421 accggatctg gtcggatcaa agggattttc aagcgaggag atggccggca tgctctatga
      481 cagcctgcat caaaagttgc ttaccctcga tgactcagtt gaagtctacc cggcccatgg
      541 ggctggatca ctgtgcggga aaaacctttc aactgaaact tcctcgacca ttggtgacca
      601 gcgccggttg aattacgcgt taaaaccaat gtcacgagcg gcatttgtcg ccatgatgac
      661 ggctgattta ccggaagttc cagcctattt cccgcttgat gctgagatca atcgaactgg
      721 cgcttcacca ctggtggagc tgggcgccgc cagggcgttg acacctgagg aagggaataa
      781 actgatccag caagggtatc tggtcctcga tattcgaccg gccccccagt ttggaagtgg
      841 ctatatcccc ggttcgctca atatcgggct gaaaggacaa tttgcctcct gggcggggag
      901 cctgattccg ctcacaaccc cactgctgtt agtggttgaa tcacctgatc aggtcaccga
      961 agcggtcacc cgactggcca gagtggggat cgaaaccgtc ggcggatacc tggctggtgg
     1021 gattgatgcc tggaaagccg ctggtctcga actcaagaca gtccctcaac tctcagttca
     1081 cgcactcgcc agtcgcctgg aagccgaacc cgacctgcag gtggttgacg tgcgccgccc
     1141 actcgaattt tgccaggggc acgttccaac cgcactgaat ttccaactgg cccaactgga
     1201 gaaaaacctg gatcacctcg acccggcccg gccaaccgct gttatctgtg cgggtggata
     1261 tcgttccagt gccggggcca gtctgctttc caggcatgga ttccatcaag tgttgaatat
     1321 cgtgggcggc acggcggcct ggattcaggc cgggctcaag gttgaggctg agatcgaatc
     1381 tgctgcctgt tcataagctt tccctgtttc ggcgtctcct gacatctggt tgatcgctgg
     1441 tctgtcctat tgaagtaaag gcacacactc ggagaaaaga tcagacctca aggcgccaga
     1501 attcagctcc gtttcctgaa tttgattgaa tctggcacga tgcttgcaca aattatcgaa
     1561 atttgatcct cattggtgaa ggaacgatcc ggttatgcca gttttcgagt taaaccaatc
     1621 tgatacttcg gtaccggtgc tggaattccg acacgttgcg ttaagttttg gcgacactga
     1681 agtcctgcga gatttgagtt tcaagctgca tcaaaacgag ttgatttgtc tgacagggac
     1741 tgctgggact ggaaaaaccg tgacgctgat gctggccatg gggctactgc aacccgacga
     1801 aggtcagatt ttaatctatg gcaaagaaat tgagcatcta tctgaggagg aattgctggc
     1861 gctccggagc cgtcatttgg ggattacctt tcaggaagat gcgttattta ccagcctttc
     1921 cgtttatgaa aatgcggcct accgactggt tgaacaaggc tggtcagagg gtgcgattcg
     1981 aacggcagta agtgaaatta ttgccatggt tgggctgacc gatgccatgt ggaaaacacc
     2041 tgaagaatta tctggcggta tgaagcggag actggaaatt gcccgggccc tggtgggctg
     2101 gccatcaatt atgttatttg atgagccaac cgccgggctt gacccggtaa atgcgaaaaa
     2161 catcctggac ttgatcttac aggcgcgtga tttgcatcag acatcggcaa tttttgtttc
     2221 aaaggaattg cacattattc cctatctggc cactcatcga gcccaacacg atccggaaca
     2281 gggtgaggtc cagattgtgc gtgccagaca cgataacttg ccaaatacgc acgttctggt
     2341 tttatctgag ggggttgtct ctttttgggg aacggtggag gaattttcag ccagtcatgc
     2401 tgcttcagtt ctcccgttaa cgcatcctga gcaaattccc cacgagcctg aaatctatat
     2461 tccgattaca acccaatcgg ttcgaaaaca aacccgggat tccgaaaatt cctaaactgc
     2521 tttgcattca actctattgc tttcatgaaa ccctattcga attcttctga aactgattta
     2581 gaccgcctca ctgcagaatt agagcgcttg cgtgaagaga acaaacgtct gcgggctgag
     2641 aattcgaaat tgcagtatcg cgagaccctg ctgacccaaa aagccagatc actcaatctc
     2701 attaccaaag ccaccagagt cggtatctgg acctggaatc aaaaaaccga tgaggtgcac
     2761 tggtcagatg agtgttatca gttaatgggg ttggaaccag ccgagacaga gttaacgatt
     2821 tcgttatggc gaaagtgtat ccacactgag gaccattcac agttgacgga ggtggccgat
     2881 tcgtccttag aaggacattc gtttggtgac tatgaatatc gggttgtctg gcctgacgga
     2941 accgtccggt ggattagtga aaaagcccgg ctctttcggg atttagatgg catgccaggt
     3001 gagatactgg gcggcgtcat gattgatgtt accgaccgaa agctggcaga aatggcatta
     3061 caggaaagcg aatcccggta tcggaccatc tctgaaatta cttcagatta tacctattca
     3121 ttccggatca aacctgacaa gtcatttgaa ttggaatggg ttaccgatgc aatctatcgg
     3181 atcttcgggt atccgaaaga cgtgataagt cagcctgaaa actggtggca actggttcat
     3241 ccagatgatc gggtgttatt ccaacaaacc ggggaaagta tctgggctgg tcgtcccatt
     3301 gtccgggaat tgcgaatggt gaccattcac gggcaaattc gctgggttcg agaccatgtc
     3361 cggcccattt tggatcagaa ccagcaattg attcggatta ttggggccac ccaggacgtg
     3421 actgagcaaa aatatgccat cgaggccctg cgccgaagtg aggcccaaat tcgtcaaatt
     3481 attgatgtca ttccaggtgc cgttttccaa ctccaggaaa ccgatgatca ttcgccggtg
     3541 gtttcgtttg tcagtgcaag agcctatgac ttgctcgggt ggtcaactca agagattcgc
     3601 ctgctcacaa ctcagggaaa tgtgttgatt catccagatg atcaacctca ggcataccgg
     3661 tcctatctcg aagcccgcca gacccgtact acccttgacc ttgagtttcg agtcctgacc
     3721 caatcccagg aatataaatg gattcgaacc tgtgccgtgc cgctcaaaaa atcacctcac
     3781 gtagttgagt gggttggaat ttcatttgat atctcgcggc aaaaagaaaa tgaggaagac
     3841 ctgcggtatc atcgcgaatt atttgaaacc atctatacca actcaaccga tgccatcttt
     3901 ttggtagata cacaaacgga cttcattatt gactgcaacc agcggggagt tgagttattt
     3961 gaatatccag agaaaaacaa actgattggg ttgcgagggg gggcatttca taaagtcccg
     4021 ctctcagaag ctgaaagtcg caagtttggg actgagattc aaagtggacg aacggtaacg
     4081 gctgaatttg aatatgtgac ccaatctggc cggtcctttt ggggaagttt ggccagtaaa
     4141 atgatatcgg ttggtggcaa gcgactcaat ctggtccgga tcaccgatat caccagccgc
     4201 aaacaaatgg aggaagaaca gtacaaaatc gccaaactgg aatcacttgg agttctggcg
     4261 ggggggattg cacacgattt taataatttg ctcacggcca ttattggaaa tgtcgaactg
     4321 gtg
//
LOCUS       DAOQDO010000209         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13937_length_4312_cov_3.183463, whole genome shotgun sequence.
ACCESSION   DAOQDO010000209 DAOQDO010000000
VERSION     DAOQDO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13937_length_4312_cov_3.183463"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1463
                     /gene="fabF"
                     /locus_tag="PKZ53_04630"
     CDS             207..1463
                     /gene="fabF"
                     /locus_tag="PKZ53_04630"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNJ39753.1"
                     /translation="MQNAANGARRVVVTGIGLVTGIGNTREENWTSMMEGQSGAGPIT
                     HFDASGYPVRFAVEVKNFDLSKYFDHREQRRTAPYIHYAVAASEEALVDSGLQVTPDN
                     AERIGVYISSGIGGFGIIEREHSRLMKEGPRHISPYFMISYLVNMADGYVSIRHGLKG
                     PSSATATACAAGVHAIGESFRLIQDGDADVMVCGGSEGAITPMGVGGFSAAKAMSTNN
                     DNPKGASRPFDLNRDGFVLGEGAGIMVIEEYEHARARGAKIYAEVVGYGMSADAHHIT
                     TPAPNGDGVYRMIRNVLRDAQVEPEVVDYINAHGTSTPYNDKFETLAIKRVFGDHSYK
                     LAVSSTKSMTGHALGAAGGIEACYTALAVERQILPPTINYETPDPECDLDYVPNRPRP
                     AKITYAMSNSSGFGGTNAGLLLKRVE"
     gene            1491..2087
                     /locus_tag="PKZ53_04635"
     CDS             1491..2087
                     /locus_tag="PKZ53_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39754.1"
                     /translation="MSSQRKPKYTASEYLQLERSASERHEFAFGEIFAMGGASARHVE
                     IVSNLVRELGNRLRDRTCKVYATDLRLCVDAEGRYTYPDVLIACGKPRFLDDQFDTLL
                     NPDVIIEVMSPSTRNYDRGDKFQQYREIPSFHEYLLIDQSCVYIEKHTKGDDGDWKLT
                     EIKSLEAEIHLDSINIVLPVSEIYLKVDFAPVEDATGL"
     gene            2120..2563
                     /locus_tag="PKZ53_04640"
     CDS             2120..2563
                     /locus_tag="PKZ53_04640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ39755.1"
                     /translation="MVKSSFKNPPVHQSALDQLPPVICQQLAALKPEQFPFSLMSINI
                     RRFQVFNDAYGFEVGDRALHQLVEVVKTVSGAKAFELFKAHNLVVFYPHTDESQIKHL
                     AQAIKNQFQALAIPFQHPEVKIEAGILTIRTEFATCHEKPMTGTR"
     gene            3235..3684
                     /locus_tag="PKZ53_04645"
     CDS             3235..3684
                     /locus_tag="PKZ53_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HNJ39756.1"
                     /translation="MEKFDWSRINHLQLGKYAEYFAKMQFVLHGYDVYSSEVDDRGID
                     FVIRKNHDCYYDIQVKSVRGFGYIFFTKDKFSPRHNLFAVIAVFFNGALPQLYLIPST
                     AWPSENKVLVSRDYEGKKSKPEWGIQISKKGLEYLEQYAFDQRVMQL"
     gene            3790..>4312
                     /locus_tag="PKZ53_04650"
     CDS             3790..>4312
                     /locus_tag="PKZ53_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ39757.1"
                     /translation="MVTPVSKNLLKHLRPSDVRGLAQLATQATTSVTRITEGVHQSVL
                     STMGFPGGKEHGKTRGFTGLIYKSIHGVTNLVGKSVDKVLESLQPLLETPEDDKPGTP
                     QREAVLAVLNGVMGDRLVESASPFAIPMTMNYQGNVLNWEDLPSIPEATGKVLLLIHG
                     LCINDLAWHTEIKD"
BASE COUNT         1115 a         1051 c         1075 g         1071 t
ORIGIN      
        1 gagcccgatg tcttctgccc caagccctga gcccgatgtc ttctgcccca agccctgagc
       61 ccgatgtctt ctgccccaag ccctgagccc gatgtcttct gccccaagcc cgatgccttc
      121 tgctcttgct ttccttgcgt caagaaagat aaattgcgcg actctaattt cgcactcaaa
      181 tcattctatt tagaaggaaa gtctgtatgc agaacgctgc caatggtgct cgtcgtgtcg
      241 tggtgaccgg gattgggctg gtcactggaa ttgggaatac tcgcgaagaa aactggacct
      301 cgatgatgga aggccagtca ggtgctggac cgattacgca ttttgacgct tcagggtacc
      361 cggttcggtt tgccgttgaa gtcaaaaatt ttgacctgtc gaaatacttt gatcatcgcg
      421 aacaacgacg cacggcgcct tatattcatt atgccgtggc ggcttctgaa gaagccctgg
      481 tagattctgg tctgcaggta acaccagaca atgcggagcg catcggggtc tatatcagca
      541 gcgggattgg tggctttgga atcattgaac gtgagcactc ccggttgatg aaagaaggac
      601 cgcgtcatat ttcgccatat tttatgattt cttacctggt gaatatggct gatggctatg
      661 tttcaatccg gcatggctta aagggcccaa gttcggcaac cgcaaccgcc tgtgcagcag
      721 gggttcacgc gattggcgag tcgttccgac tgatccagga cggagacgcc gatgtgatgg
      781 tgtgcggcgg gtctgaaggc gcgattaccc ccatgggggt tggcgggttt agcgctgcca
      841 aagccatgtc aaccaacaat gacaatccaa aaggtgcgtc acgtcccttt gacctcaatc
      901 gcgacgggtt tgtgctcggc gagggcgccg ggattatggt gattgaagaa tatgagcatg
      961 cgcgggctcg cggggccaaa atttatgccg aagtggtcgg ctacggcatg agcgctgacg
     1021 cccaccacat cacgacacct gccccgaacg gagacggcgt gtaccggatg atccgcaatg
     1081 tgctgcgcga cgcccaggtt gaacccgaag tggtggatta catcaacgcc cacgggacgt
     1141 ctacgccata caatgacaag tttgaaacgc tggccatcaa gcgcgttttt ggcgatcact
     1201 cgtacaaact ggcggtgagt tcgaccaaat ccatgacggg acacgcgctg ggggcggcgg
     1261 gcggcatcga agcctgctac acggcactcg cggtcgaacg acaaatcctg ccgccaacga
     1321 tcaactacga aacacctgac ccggaatgcg acctggatta tgtgcccaat cggccacgcc
     1381 cggcgaagat cacctacgcc atgtccaatt cctcgggctt tggcggcacc aatgccgggc
     1441 tgctattgaa acgggttgaa taaagtaccg atctttggag aaatttcacc atgtcgtcac
     1501 aacgcaaacc caaatacacc gcctccgaat acctgcaact ggagcggagt gcttctgagc
     1561 gacacgaatt tgcctttggt gaaatttttg ctatgggtgg tgcttctgcc cgtcacgttg
     1621 aaattgtgag taatctggtt cgtgaattgg ggaaccgtct tcgtgaccgg acctgcaaag
     1681 tctacgccac tgacctgcgg ctctgtgtcg atgccgaagg ccgctatacc tacccggatg
     1741 tgctcattgc ctgcgggaaa ccacgctttc ttgatgatca gtttgatacc ctcctcaacc
     1801 cggacgtcat tatcgaagta atgtcgccgt ccactcggaa ctatgatcgg ggcgacaaat
     1861 tccaacagta tcgggaaatc ccttcttttc acgaatatct gctgattgac cagtcgtgtg
     1921 tgtacatcga aaaacacacc aaaggcgatg atggagattg gaaactcact gaaataaagt
     1981 cgcttgaggc tgaaattcac ctcgactcaa tcaacattgt gcttccagtt tctgaaatct
     2041 atctgaaggt agattttgct cccgttgaag atgctacagg gttgtaacaa cttcgtagtc
     2101 tcaaacttca ggagtaaaaa tggttaagtc cagctttaaa aacccgcctg tacatcaatc
     2161 agcacttgat cagcttccac cagtcatttg ccagcaactt gcagccttaa aacctgagca
     2221 gttcccattc tctctgatgt cgatcaatat tcggcggttc caggttttta atgatgccta
     2281 tggatttgag gttggagaca gagcgctgca ccagcttgtt gaggttgtca aaactgtttc
     2341 aggagccaaa gcattcgagt tgttcaaggc acataatctg gtggtctttt atcctcacac
     2401 agatgaatct caaatcaagc acctggctca ggcaatcaag aaccagtttc aggcgctggc
     2461 gattcctttt cagcatccgg aagtcaaaat tgaagcaggt attctaacaa tcagaaccga
     2521 atttgccaca tgccacgaga aacctatgac aggtacaagg taagatgccc ccagccaatt
     2581 actttcggca ataaccattg ccgttaaacc ttcactgctt ttcagttcat ccttaagaaa
     2641 tatgaacact caacaacaag aaatcctgaa tcagcttgag gaatttggct ggcaagttgt
     2701 aaccagagaa gatgcacccc ttgattggtg ggctgatgag atttggctcc tggcatcaat
     2761 ttggtcacca acagattgta agatttatat tacattcctg gttgacccaa tgtgggaaac
     2821 tccccgtaag aaatctgagg gagtctgggc ggtaaaagct tcactcgaac gccccacagg
     2881 ctaccaatgc caggcaacag aagtagaaat aagtttggga cgtgggtgga aggagagatt
     2941 gcctgaattt ttcgagagtt tgtcgaagtt caggtcagcc tggaaattgc gatgtcagga
     3001 gtcgtcaact attggcaatt gatcgcctca ttactgagaa aactatgaat ttactccaaa
     3061 agtatctgaa tgaagcgctt gaatcagggt gattcgttga cgtgtatcgg gataatcgcg
     3121 atgattcagc caggttttgc ctggatagat agcggaccac aattgagttt gggagcttca
     3181 cagtaaactc aaagagttaa atttttgcgt attttccaac atacaggtgc ataaatggaa
     3241 aaatttgatt ggtcacgaat aaatcatctt cagcttggca aatatgctga atactttgcc
     3301 aaaatgcagt ttgtgctgca cggttatgat gtttattctt ctgaggttga cgaccgagga
     3361 attgattttg tgattcgcaa aaaccacgat tgctattatg atatccaggt caaatccgtg
     3421 agaggatttg gatatatttt ctttacaaaa gataaatttt ctccacgaca taatctgttt
     3481 gcagtcattg cagtgttttt taacggagca ttgccacaac tttatttaat tccttcaact
     3541 gcctggcctt ctgaaaacaa agttctggtc agtcgtgatt atgaaggaaa gaaaagcaag
     3601 cccgaatggg gtatccagat ttccaagaaa gggcttgaat atttggagca atatgcgttt
     3661 gaccagcgag taatgcagtt gtaaatgctg gttggtgagt cagggattcc ctcttgaacc
     3721 aagcccatca ttcagggatg atgaaatccc ttccgtttcc ccaaaattga tctatggagt
     3781 ccactcaata tggttactcc agtctcaaaa aatctgctca aacatctgcg tccgtcagac
     3841 gtccgagggc ttgcccaact ggcaacccag gccaccacca gcgtcacccg cattaccgaa
     3901 ggagttcacc agtccgtgct cagcacgatg ggctttcctg gcggcaaaga gcatgggaag
     3961 acccgtggat tcactggcct gatttacaag agcattcacg gtgtcaccaa tctggtcggc
     4021 aaaagtgtgg ataaggtcct ggagagcctt cagccgctcc ttgaaacccc ggaagatgac
     4081 aaacctggaa cgccccagcg cgaagcggta cttgccgtcc tcaatggcgt aatgggtgac
     4141 agactggttg aaagtgcgag cccgtttgcc attccaatga caatgaatta tcaaggcaac
     4201 gtactgaact gggaggattt gccgtcaata cccgaagcaa cgggcaaagt gctgcttctg
     4261 attcacggtt tgtgcatcaa tgacctggcc tggcacaccg aaatcaaaga tc
//
LOCUS       DAOQDO010000210         4311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13941_length_4311_cov_3.750470, whole genome shotgun sequence.
ACCESSION   DAOQDO010000210 DAOQDO010000000
VERSION     DAOQDO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_4311_cov_3.750470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..792
                     /locus_tag="PKZ53_04655"
     CDS             283..792
                     /locus_tag="PKZ53_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ39758.1"
                     /translation="MLKRLVVGVCLLFWSAFVFVNESQSLISFSVTAAQNPPSVPAQE
                     PTKTIDKEKIVQDLLKKIAGQENKPAEEVFKNIQLLKGIPAGRLVLMMNMGYSRALGV
                     DCTHCHTLDQWEKDEKPEKLIAREMVKMTSTINNEQLKNIKNLKGPNPVINCTTCHRG
                     QTKPALNLK"
     gene            802..1002
                     /locus_tag="PKZ53_04660"
     CDS             802..1002
                     /locus_tag="PKZ53_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39759.1"
                     /translation="MVSLGTPASCRQLIGSEPLGAGKMPAFPGNIYLKNYVDVNLVVS
                     LMSWMHAKARTLEFGGAEYVAH"
     gene            1329..>4311
                     /locus_tag="PKZ53_04665"
     CDS             1329..>4311
                     /locus_tag="PKZ53_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39760.1"
                     /translation="MDRRLLIPPMLVLCLLGYAWWGESRFVSATPAPIPQVPSKQVPA
                     LATPDGFPGGRPGMHIFSNQDGLPQNSILSLTTDQKGYLWAGTQDGAACYNGHRWTTV
                     NMPNRMRSNYVRTVFAASDGSIWFGTTGNGAIRLQNGNWTTFTTEAGTVPTDNVFCFE
                     ETQGAAGNTILWVGTSGGLATFENNQWQTLTPENSGLPHKAINILLKTKAIDGSVVLW
                     AGTSGGLARLAQGKWTAFTAGTSPLPHNQVLSLLETTESPGKQALWIGTAEGGLARLT
                     NDQWTVFDPQQTGLLKNRVLGLVETIEPDQTHTLWIGSYGSGLVRYRDGKWVTYNRQT
                     SALPNDAVMSLYKSVTPDGAYTIWTGTVNGLARLRSGTWTTYAPATVGMANNVVLSLL
                     ETRSATDQRVLWLGTAGGGLARLENGQWTSYTTQNSGLPNNRVLCLLESREPDGRQAI
                     WMGMNGGGLACLRGNQWTVYTKENSKLPSNLVWSLTQTVEADGTPVLWVVTVGGGVVR
                     IKGSDWTLLNHQNSGLPSDDVLALLITNADDGSPMLWFGTDGKGLVRFHRNQWTVFDT
                     QNSGLANNFVWSLFESTASDGHRTLWIGTGGGGVSIFDPTSTGPQWRTISDQNLPGFP
                     NNTINQICQDQKQRMYLFTNRGVARLTPTGPRAWNPDEFSLYTFTTDDGLPSNECNQG
                     AAMVDRQGCIWAGTLEGAAMLDPGTEFIDRSPKQLLLERTLLNGKLFDRSPDGVPQNV
                     VFEYNENNLVFEYALLSYFREAETRYQTQLVNFDEQRSEWKVDTKKEYTSLPEGNYTF
                     QVWARDYAGNVSGPVTATFRVLPAPWRTIWAYFFYAGAVFGIGYAIYSWRVQALRRRQ
                     AEKVAHLRQLQEERIAHLHQLQEERISSLRHLLESIRVINLPLDLNTVLQNIAQESAQ
                     LIEGEPGGIGLIEDEQVVFRRLWMRDHWVDEPLIFQFGEGVAGRVAATAKPLIVNDSE
                     HSGEIAFHTLIKKFDVLGMMDV"
BASE COUNT         1054 a         1056 c         1189 g         1012 t
ORIGIN      
        1 ggctgaagaa gacaggctga gaaaaccagg gctgaagaaa accagggctg aagaagacag
       61 gctgagaaaa ccagggctga agaagttggg ctgaagaaga tgggctgaag aagatgggct
      121 caggggattg aacccggttt cttcagcccc gagcgtgcga gtcttcagcc ccaagcccgg
      181 agcccaatat cttcagtccc aagccctgag ccctggtgtt tgcagtccca caattccaat
      241 tccaggccac acaacatatt tcttgcagga gtttccaacc ccatgctcaa acgtctggtt
      301 gttggtgtgt gtcttttgtt ctggagtgca ttcgtttttg tgaatgaatc ccaaagtttg
      361 atcagttttt cagtaactgc cgcccaaaat ccaccttcgg ttccggccca ggaaccgacg
      421 aaaaccattg ataaggaaaa gatagtccag gacttgctca aaaaaattgc cggtcaggaa
      481 aacaaaccgg ctgaggaagt cttcaaaaat attcaattgt taaaaggaat tccggctggg
      541 cgactggtgc ttatgatgaa tatgggctac agccgggcac tgggagttga ttgcacccat
      601 tgccacacgc ttgaccagtg ggaaaaagat gagaaaccgg aaaagctgat tgcccgtgag
      661 atggtcaaaa tgaccagtac gatcaataac gagcagttaa agaacatcaa aaacctgaaa
      721 ggcccgaacc cggttatcaa ttgcaccacc tgccaccggg gccagaccaa accggctctg
      781 aatttgaagt aagaccagtt tgtggtcagt ctgggaacgc cggcatcctg ccggcaactg
      841 attggatctg aaccgcttgg tgccggcaag atgccagcgt tcccaggaaa tatttatctg
      901 aaaaactatg ttgatgtgaa tttagttgtc tcgctaatga gctggatgca cgctaaagca
      961 agaactctgg aatttggagg cgccgaatat gtagctcatt gaaggcttag aaatcattga
     1021 agactttgga tttattttct gtgtattccg tgtattccgt ggtttcttat tctgaaaatc
     1081 cataccaaaa ctgctctttt atcccactgt cttcttgccc tccctgtcgc ctccggtacc
     1141 tattcaggcg aaataacttc tggattccac gcagaacttg tcatattgtt cagactgagt
     1201 taacaccatc tcctggggtg tcagccgaca tcaaaaccgg ctaaactctt ttcaggtaca
     1261 ttttcctcga ttttccaaca cgcaaccagt tttcctttct tcaaagcaac aactttttgt
     1321 gccgcattat ggatcgccga ctcctgattc cccccatgtt ggtgttgtgc ctgctgggct
     1381 atgcctggtg gggtgagtcg cggtttgtgt cagcaacgcc agccccgata ccccaagtgc
     1441 catccaaaca ggtgccggct ttagcgacac cagatgggtt tccaggtgga cggccaggga
     1501 tgcacatctt tagtaatcag gatgggttgc cgcaaaattc gattttatcc ctcaccactg
     1561 atcaaaaagg gtatctctgg gctggcaccc aggacggggc cgcctgttac aacggccatc
     1621 gttggaccac ggtcaatatg cccaatcgga tgcgatccaa ctatgtccgc acggtgtttg
     1681 ccgcttcgga tggcagtatc tggtttggaa caacgggcaa tggggcgatt cgcctgcaaa
     1741 atggcaactg gacaactttt acaaccgaag ctggaaccgt gccgactgac aatgtatttt
     1801 gttttgaaga aactcagggg gcggcgggga acaccattct gtgggttgga acgagcgggg
     1861 ggctggccac ttttgaaaac aatcagtggc agacgctgac acctgaaaat tcgggcttgc
     1921 cgcataaagc gatcaatatc ttactgaaaa caaaagcgat agacggttcg gttgtgttgt
     1981 gggcgggcac cagtggagga ctggcacgcc tggctcaggg aaaatggacg gcgtttactg
     2041 ccgggacatc tccgttgccg cacaatcagg tcctcagttt gcttgaaacc accgaatcac
     2101 ctggaaaaca ggcgctgtgg attggaacgg cagaaggggg actggccagg ttgaccaatg
     2161 atcagtggac ggtttttgat ccccagcaaa ccggattgct caaaaaccgg gtcctggggc
     2221 tggttgaaac gattgaaccc gatcaaaccc acacgctgtg gattggaagt tatggcagtg
     2281 gtctggtgcg ataccgggat ggaaaatggg tcacgtacaa tcgccaaacg tcagctttac
     2341 caaatgatgc cgtgatgagc ctgtacaaat cagtgacacc ggacggggcc tatacgatct
     2401 ggactggcac cgtcaatgga ctggcccgac tccggtctgg aacctggact acctatgcgc
     2461 cagctacggt tggcatggca aacaatgtgg ttttgagcct gttggaaacc cgatcagcaa
     2521 ctgaccagcg ggtcctctgg ctgggaactg cgggtggcgg actggctcga ctggaaaacg
     2581 gccagtggac cagctacacg acgcaaaatt ccggtctgcc aaacaatcgg gtgctctgtc
     2641 tgctggagag tcgtgaacct gatggacgtc aggccatctg gatgggcatg aacggtggcg
     2701 gtctggcctg cctgcgtggc aatcaatgga ctgtctacac aaaggaaaat tcaaagctcc
     2761 cgagtaatct ggtctggagc ctgacccaga cggtggaggc ggatggcaca cccgtgctct
     2821 gggtcgtgac agttggtggc ggtgtggttc gcatcaaggg atctgactgg accctgttga
     2881 atcaccagaa ttcgggtctc cccagtgatg atgtgctggc gctcctgatt accaacgccg
     2941 atgatggttc accgatgctc tggtttggaa ctgatggaaa aggactggtg cgatttcacc
     3001 gcaaccagtg gacggtcttt gatacccaaa attccggcct ggccaataac tttgtctgga
     3061 gtttgtttga atccacggca agtgacgggc atcggacgct ttggattggc actggcggtg
     3121 gcggcgtgag tatctttgat ccaacttcga ctggtccaca atggcgaacc atctctgacc
     3181 aaaacctgcc tggatttccc aacaacacca tcaaccagat ttgccaggac cagaagcagc
     3241 gcatgtattt atttaccaac cggggcgtgg cccgtctgac gccaaccggg ccgcgtgcct
     3301 ggaacccgga tgaattttca ctctacacgt ttaccacgga tgatggcttg cccagcaatg
     3361 agtgcaatca aggggccgcc atggtggatc gccaggggtg catttgggcc gggacgctgg
     3421 aaggggcggc gatgcttgac ccaggcactg aatttattga ccgctcgcca aaacaactcc
     3481 tgcttgaacg cacgctgttg aatggaaagc tgtttgaccg atcacccgac ggtgtgcccc
     3541 agaacgtggt ctttgagtac aacgaaaaca atctggtgtt tgaatatgcg ctgctgagct
     3601 atttccgtga agccgaaacc cgctaccaga cccagttggt gaattttgac gagcagcgtt
     3661 cagaatggaa agttgatacc aaaaaagaat atacctcgct gccggaaggt aactatacct
     3721 tccaggtctg ggcacgggat tatgctggga acgtgagcgg accggtgacg gcgacgtttc
     3781 gagtgttacc ggcgccctgg cgaaccatct gggcctattt cttttatgcc ggggcggtct
     3841 ttgggattgg ctatgccatt tattcgtggc gtgtgcaggc tttgcggcga cgacaggctg
     3901 aaaaagttgc ccatttgcga caattacagg aagagcgaat tgcccatctc catcagcttc
     3961 aagaagaacg aatttccagc ctgcggcatt tgcttgaaag cattcgcgtc atcaacctgc
     4021 cgcttgatct gaataccgtt ttgcaaaata ttgcccagga aagtgcccag ttaatcgaag
     4081 gggaaccagg tgggattggg ttgattgaag acgagcaggt tgttttccgg cgtttgtgga
     4141 tgcgcgatca ctgggttgat gaaccgctca tttttcagtt tggcgaaggg gttgccggtc
     4201 gagtcgccgc caccgcgaaa cccttgattg tcaatgattc agagcattca ggggaaatcg
     4261 cttttcatac gttgatcaag aaatttgatg tcctgggaat gatggatgtg c
//
LOCUS       DAOQDO010000211         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13972_length_4305_cov_3.392471, whole genome shotgun sequence.
ACCESSION   DAOQDO010000211 DAOQDO010000000
VERSION     DAOQDO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13972_length_4305_cov_3.392471"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1233
                     /locus_tag="PKZ53_04670"
     CDS             145..1233
                     /locus_tag="PKZ53_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39761.1"
                     /translation="MDSLVVSNVQQRPTRTAVSILGVALGVVLVLIFVGLVNGMQHSR
                     AQRDSNVGADIMFCREFSLTTTSLSLPTQYQAELNKVPGVTVTTPVGQYLKESKNGIG
                     FEILEGIDFPSYAKMSGITMKVGQPLQNELDVIVDEDYAKHKKVTVGSEIELFNRKFK
                     VSGIYQPESGARIKINLASMQEILSSPERCTVIYVKGTDSESQASLAKKIDTALPGNK
                     IMPMEDLLQLYENGIPAFNQFLKVVVGLAIIVSLLVVLLAMYTTITERTREIGILKSL
                     GASKAFIIREIEQEALLICALGVVVGFLFSFLLKVGITKFTSLQVELEPRWMLYAALI
                     GLSSGLLGALYPALRAANQDPVDALSYE"
     gene            1302..2045
                     /locus_tag="PKZ53_04675"
     CDS             1302..2045
                     /locus_tag="PKZ53_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ39762.1"
                     /translation="MKTILQAIDVSMIYRAGKVDVPALRGINLEVKEGEFVAVMGQSG
                     SGKSTLLHILGGLLKPTKGTVLVDGEDLAKASDAYRTDIRRRKIGIVFQRFNLFPTLS
                     VEGNLKLAERIRQKGSQNEAPAQEYRRHILQLLSLENRLHHKPIELSGGEQQRVALAR
                     AIVNHPAILLADEPTGNLDSENSQIVLDMLVKLNKELGQTVLMITHNLEAAQTANRLV
                     TMRDGRILHDVPVQKSALSAVEPGVLAGK"
     gene            2091..2429
                     /locus_tag="PKZ53_04680"
     CDS             2091..2429
                     /locus_tag="PKZ53_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39763.1"
                     /translation="MRKIFEVIKATVLWNYERGTWQYDVLCVAILAFIFLIPTSFFDE
                     RKSRSLKTPDYISTSELQNQSPAPTLKVLLESAGSRRRGGQEVKLKHFEAVSNEDGVI
                     VGYQVWYQQN"
     gene            2480..3181
                     /locus_tag="PKZ53_04685"
     CDS             2480..3181
                     /locus_tag="PKZ53_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNJ39764.1"
                     /translation="MKLKALFSVILFIGITAISVIPGNFAAVQANAPADALNTVLANI
                     ETTSRQMTGLQATVSHQRTNGQLNIKDPKQVGTLLYKPGSVRRMRIDYTQPQPKVVAV
                     NGDKGLLFERNLNQVFLSTVSKVASKAGSSGILSILNSAAQLKEKFDIKLVGNEGFNG
                     MQTTHLTLTPKAQDGFTRIEVWIDTRTWLPVRQIFYERNRDFTEVTLSNIIFNPKLSD
                     KQFDVDYGKAKVIKG"
     gene            3501..>4305
                     /locus_tag="PKZ53_04690"
     CDS             3501..>4305
                     /locus_tag="PKZ53_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNJ39765.1"
                     /translation="MADQILIDQDPQDLTGEDAVKIPEVLPVLPLRDIVIFPFMIVPL
                     FVQRERSIRSVEQVLSDNRLILLLSQRDGDKDEPVEQDLHQVGTVAVVMRMMKLPDGR
                     IRILVQGISRALVKKIEETPAYLRATITPLTEPKLESSLELEALTRNIKNSMEKAITL
                     GKNISPEVMAITNSLEDAGRLADLVASNLELKVAEAQTVLEQIDPSQRLRRVNELLAR
                     EISVLQVQQEINSQAKGEIDRSQREYYLRQQLKAIQQELGEGNELLEEIN"
BASE COUNT         1160 a         1040 c         1069 g         1036 t
ORIGIN      
        1 aaatagaatc aactacactt aacagataat ccaaaatccc aacactcgac gactgacgac
       61 tgaccactga ctactaactc atcagctact gacgactgac tcatcagcta ctgacttttc
      121 atcttccgtt ctctttaaat cattatggac agtttagttg tttcaaatgt gcagcagcgc
      181 cccacccgaa ccgccgtgag tattcttggg gtggcgcttg gggtggtact ggttttgatt
      241 tttgttggac tggtcaacgg catgcagcat agtcgtgcac aacgtgactc aaacgttggg
      301 gctgacatca tgttttgccg tgaattcagc ctgaccacaa cctcattgtc tcttcccacc
      361 cagtatcagg cggaactgaa caaggtcccc ggcgtcacgg tcaccacacc cgtcggccag
      421 tatctcaaag agagcaaaaa cggaattggg tttgaaatcc tggaaggcat tgattttcca
      481 agctatgcca aaatgtcagg catcaccatg aaggtcgggc aaccattgca aaatgagctg
      541 gatgtcattg ttgatgagga ttacgccaaa cacaaaaagg tcacggtcgg cagcgaaatc
      601 gaactcttta atcgcaagtt taaagtcagt ggtatctatc agcccgaaag cggcgcccgg
      661 atcaaaatca atctggccag catgcaggaa atcctttcat ctccggaacg ctgtacggtg
      721 atctatgtca aagggactga ctcagaaagc caggcttcac tcgcaaagaa gattgatacg
      781 gcactgcccg gcaacaagat tatgccgatg gaagacctgc ttcagctcta tgaaaatgga
      841 attccggcct ttaatcaatt tctaaaagtt gtggttgggc tggcaatcat tgtaagctta
      901 ctggttgtct tactggcgat gtataccacc attaccgagc gaacacgtga aatcggcatc
      961 ctcaaatcac ttggggcctc aaaagcattt atcattcgcg aaatcgaaca ggaagccctg
     1021 ctgatttgtg ctctgggggt tgtggttgga tttctctttt cattcttgtt gaaagttggg
     1081 atcaccaaat tcacctcgtt acaggttgag cttgaacccc gctggatgct gtatgccgca
     1141 ttgattgggt tgagtagcgg attgctcggg gcgctttacc cggcactacg tgccgccaat
     1201 caggatcccg tggatgcctt gagctacgaa tagctcatcg ggtgctcctt aaaaatttag
     1261 aaagcaggct ttgctcagtt cagaaagtca atttgaggtt tatgaaaaca attctgcaag
     1321 ccattgatgt ctctatgatt taccgggccg ggaaggttga tgtgccagcc ctgcgtggaa
     1381 tcaatctgga ggtgaaagaa ggcgaatttg tggctgtgat gggacagtcc ggttcgggca
     1441 aatcaacgct cttacatatt ctgggtggtc tgctcaagcc aaccaaaggc acggtcctgg
     1501 tggatggaga agaccttgcc aaagcgtccg atgcctatcg aactgacatc cgccgtcgca
     1561 aaattggcat tgtttttcaa cggtttaacc tgttcccaac cctttcggtt gaaggaaatc
     1621 tcaaactggc tgaacggatt cgtcagaagg gctcgcaaaa cgaggcgccg gcccaggagt
     1681 atcgccgcca cattttgcag ttgctttcgc tcgaaaatcg cctgcatcac aagccaattg
     1741 agctttctgg tggcgaacag caacgggttg ccctggcccg ggcgattgtc aaccacccgg
     1801 cgattttgct cgcggatgaa cccaccggca atttggacag cgaaaactca caaatcgtgc
     1861 tcgatatgct ggtcaagctc aacaaagaac ttggtcagac agttttgatg attacccata
     1921 atctggaagc agctcaaacg gcaaaccgac tggtgaccat gcgggatggt cgtattttgc
     1981 acgacgtccc ggtgcaaaaa tcagcgttgt ctgccgtcga acccggtgtt ttggctggca
     2041 aataaagcaa gtgccttttg tcgtgagagt taaggagcgc gaatcacact atgcgcaaga
     2101 tttttgaagt gatcaaagcc actgtgctct ggaattatga gcgaggaacc tggcagtacg
     2161 atgtgttgtg cgtagccatc ctcgctttta tttttctcat cccaacttcg ttttttgacg
     2221 agcgcaaatc ccgctcgctc aaaacacccg attacatcag caccagtgaa ctccaaaacc
     2281 agtccccggc tccgacgctc aaagtgttgc tcgaatcagc cggatcacgg cggcggggag
     2341 gtcaagaggt caagctcaag cattttgagg cggtatcaaa cgaggatggg gtgatcgttg
     2401 gataccaggt ctggtatcaa caaaactagc cagtcatctg gccaggatag cgaagttttg
     2461 aggtcaaaaa acacaagtta tgaaattaaa agcacttttt tcggtcatct tgtttattgg
     2521 aatcactgcg atatcggtca ttcccggcaa tttcgcagcc gttcaggcca acgctccggc
     2581 agatgcctta aatacggttc tggctaatat tgaaaccacc agtcgccaaa tgactggact
     2641 gcaagccacg gtttcacacc aacgaaccaa cggccaactc aatattaaag acccgaagca
     2701 agttggaacc ctgctgtata agccagggag tgttcgccgg atgcggattg attacaccca
     2761 gccacaacca aaagtggtcg cagtaaacgg tgacaaaggt cttttatttg agcgaaatct
     2821 caatcaagtt tttttaagca ccgtaagtaa agtggcaagc aaagccgggt cgtccggtat
     2881 cttgtcaatt ctgaattcag cggctcaact caaagaaaag tttgatatta aactggttgg
     2941 caacgaaggc tttaatggca tgcaaaccac ccatttaacc ctgacgccca aagcccagga
     3001 tggatttacc agaattgaag tctggattga taccaggacc tggttaccag tgcgccagat
     3061 tttttatgaa cgcaatcgcg attttaccga agtgacgctg agcaatatca tttttaaccc
     3121 aaagttgtca gacaaacagt ttgatgttga ttatggcaaa gccaaggtga ttaaaggata
     3181 ggaagtcggg ctaaagacag cgggctgaag aagtcgggct gaaaaagtcg ggctgaagac
     3241 atcgggctga agacatcggg ctgaaaaagt cgggtagaag agaacgggct gaagcagatg
     3301 gatttaagga tttgtccttg atcctgaaac gcttcagccc gaagttttca gccccaagcc
     3361 cgcgagtctt cagcccgctg tctccagccc gctgtctttt gtgccgaatc cgacacagtt
     3421 ggttgagaaa ccaaagacgg aacagctatg ctgaacatcc gtcattaaaa ccgacaattg
     3481 tgctgaggag agaataccca atggctgacc aaatattgat cgaccaggat ccacaagacc
     3541 tgactgggga agatgctgtc aagattcccg aggttctgcc agtccttcca ctgcgggata
     3601 tcgtgatctt cccgtttatg atcgttccgc tgtttgtcca acgcgaacgc tctattcggt
     3661 cggttgagca agtgctttca gataaccggt tgattttatt gctgtcacaa cgtgatggag
     3721 ataaagatga acccgttgaa caggacctcc atcaggttgg aaccgtggct gtcgttatgc
     3781 gcatgatgaa gttgcctgat ggccggattc gaatcttggt gcaggggatt tctcgggcgc
     3841 tcgtcaaaaa aattgaggaa accccggcct acctgcgggc gaccatcacc ccattgactg
     3901 aacccaaact tgaatcatcc ctcgaactcg aagccctgac ccgcaatatt aaaaattcaa
     3961 tggaaaaagc catcaccctg gggaaaaaca tttctcccga agtcatggcg attaccaata
     4021 gccttgaaga tgccggacgg ctcgctgatt tggtggcgtc gaacctggaa ctcaaagttg
     4081 ccgaagccca aaccgtgctt gagcaaattg atcccagtca acgtctgcgt cgggtcaatg
     4141 aactcctggc ccgtgaaatc agcgtgttac aggtccagca ggaaattaac agtcaggcga
     4201 aaggggaaat agatcggtct caacgtgagt actatctgcg tcagcaactc aaagccattc
     4261 aacaggagtt gggcgaaggt aatgagcttc tggaagagat caatc
//
LOCUS       DAOQDO010000212         4301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_13996_length_4301_cov_2.548752, whole genome shotgun sequence.
ACCESSION   DAOQDO010000212 DAOQDO010000000
VERSION     DAOQDO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13996_length_4301_cov_2.548752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PKZ53_04695"
     CDS             complement(<1..1110)
                     /locus_tag="PKZ53_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39766.1"
                     /translation="MPRQMLGICLFAITILSFSIGLTSISARKSVDTSLKPVARLQKN
                     PSPVLALATGSSLLQPKSPDIRAVIKPVSRLQTQGVISPLFPELQSELLRAEGNLEQQ
                     IAPGLERRISGIPESAAVEWTVAPWADPGQIELEFPGVEWVETLADQTLVLHMGGSKE
                     IRFSDTTARQELPGQTVEVPVSAVYYGNGRVGIAVGIYSMSEPLVVSTRVFVSNATFT
                     VTNLNNSGSGSLRQAITDANNNGSAADTITFQSGLTGTISLTSSLPNITGTLTITGPG
                     ASTLTVNGGSFTIFSVASGITLQMSGLRVTGGNLSSGLNASQLDVGGGSATVSNCEFS
                     GSGGAAVASFVSLTMTNCSINNGGNNGLINGGTATL"
     gene            complement(1195..1392)
                     /locus_tag="PKZ53_04700"
     CDS             complement(1195..1392)
                     /locus_tag="PKZ53_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39767.1"
                     /translation="MPKVRTTRTVIKVHEVVQIQPGLLQEGDRCPLCGHEFHQVPEEP
                     QSPILRQLEEKQADPVVKTPE"
     gene            2216..4012
                     /locus_tag="PKZ53_04705"
     CDS             2216..4012
                     /locus_tag="PKZ53_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39768.1"
                     /translation="MMPKLTLLTCSLLGWLVAITFTSVPVSATCTFEPKPQQPPPQGP
                     FLHKVLSATSTDGLNWTPDNRVLLDHASVPAAIVTPNGAIRIYYVDASVPPETANVAE
                     STDGGATFKVLGLKINGLSFRKALDPCIVRLRDGRYRLYYLASEPGDTTTPHRVNSAI
                     SSDGVNFTEEQTALTYPGLVDPDVFKRPKEWLMYVFSATDGKTIVARSRDGLNFTYLN
                     PFEIQGFGTTAPIKLADGRYRLYAFNQRGQRTFVSFISNDALTWTQEPGIRLEVPQGQ
                     EITDPYVVRLPNGTWKMFYKFQDNTQSQAPARYFMSFHACDPAQGNCNDPRTHKTYLA
                     QSANGESWSLVPNWQAFPGSVPDVIRRGNKLYVFTASDEVARYNLDTQTLEPPVKVTV
                     SGLSSFGFVDPSLFVDNQGRLILFFLRGQPGGDPAQCPSGQPTCEKYFDSATEVEGTD
                     GTMFTLNADHRAVVELGGSDGLRTASDPDIFFDGRQFVLYISHGPSISVWTAPNIGDR
                     FTKVGMLSNNTGGIPSGYFDQESGRYWTFSHIIQNGKSVIRRAVHADFSRPLTETDWT
                     TVLSGTGLGLGNQVNVESPGFAINLEAQTTPQ"
BASE COUNT         1054 a         1125 c         1059 g         1063 t
ORIGIN      
        1 aagcgtcgcc gtgcccccat tgatcaggcc attattgccg ccattgttga tggagcagtt
       61 tgtcatggtg agtgaaacaa aagaggccac tgcagcaccg ccggagccgg aaaattcaca
      121 attggaaacg gttgccgagc ctccgccgac atccagttgc gaagcgttta gcccgctgga
      181 tagatttcct cctgtcactc gtaacccact catctgcaag gtaataccac tagccacact
      241 gaaaattgta aaactcccac cgttgaccgt cagcgtcgag gcaccaggcc cggtaatcgt
      301 taatgttcca gtgatattcg gcaggctgct cgttaacgag atcgttccgg tcagtccaga
      361 ctgaaaggta atcgtgtcag cagccgatcc attattattg gcatcggtaa tcgcctgcct
      421 caatgagccc gatcctgaat tattgagatt ggtcacggta aacgtggcgt ttgagacaaa
      481 cacccgcgtt gagaccacga gtggttcgct cattgaatag atgccaaccg cgattccaac
      541 tcgaccattg ccgtaataga ccgccgagac tggcacctcg acggtctggc ctggtaattc
      601 ctgtcgggcg gttgtgtctg aaaaccgaat ctctttgctg cctcccatgt gcaacaccag
      661 tgtctggtca gccagcgtct caacccactc aacgcctgga aactccagtt caatctggcc
      721 tggatccgcc cagggggcca ccgtccactc gaccgccgcg ctttccggga tcccactgat
      781 tcgccgttca agccctggcg cgatctgttg ttccaggttg ccttcggcac gcagcagttc
      841 agattgcaac tcaggaaaca gaggtgagat tactccctga gtctgaaggc gcgaaaccgg
      901 tttgatgacg gcacggatat ccggcgattt tggctgaagc agacttgaac cagttgccag
      961 cgccaaaact ggtgacggat tcttctgcag acgagccacc ggcttcaaac tcgtatccac
     1021 tgatttacgg gctgatattg atgtgagtcc aatactgaaa ctcaatatgg taatggcaaa
     1081 cagacaaatt cccagcatct ggcgtggcat ggcctgtaac tccctctgat atgcgattga
     1141 atagggggac tccttttcac aaaaaggaga ttcgttcaat aggtttttta tggttcattc
     1201 aggtgttttg acgactggat cagcctgttt ttcttccagt tgtcggagga tgggtgactg
     1261 tggttcttcg ggaacttgat gaaactcgtg gccacacagt gggcaacggt ctccttcctg
     1321 cagtaaacca ggctgaatct ggacaacttc atggactttg atcacggttc gggtggtgcg
     1381 cactttgggc atatgtctcg atacctgctt tttttggaga ccacgcgaac tgacctccga
     1441 ggacttccct tcactgaaat tgattgactg taaaaacaga gcggtttcct gtaccaacac
     1501 cctacccaat ctggcttttg aagtctcaag cgttctctca tttttctcta atttttctct
     1561 aagagccact aactccttat aaaagaatgg gattcaaaac tcatttttcc gggcacaccg
     1621 atttcagatt gtccagactc cggctttcag agtactcaca cctggtcagg tgatccctaa
     1681 agcaatcgaa tttcagccat acgcttctat tgggtgaagg gggaaatgac ggaatgacaa
     1741 gatgacagga tgacaggatg acagatgatc ggaaactccc tatttgatat caaaaattga
     1801 tttacaaaga tgatttactt atttctcaat aagttagctt ctaacagaaa atcttttgag
     1861 caggatcaaa tgacttcaga cagaacagca ggcaggtttt tttgcggcag gtcaggctca
     1921 gtaattgagg ataaatatcc cttaagctcc tcagatcaga aagcaaccac agcacaattg
     1981 tgaaacattg gattggtacc aggcatcggc gccaccacct aatttgtata ggtggtggca
     2041 cctattttgg ggctgaaaaa actttccatc accggtgacc aattgcctgt gtgaggttcg
     2101 ttaggatcat caaaccgacc tctctcaccg tccttacatt ctgaattgtg aatcccctgc
     2161 ccggctgata cttcagatca gcgagggggt tcatcattca tccaggtggt ctcctatgat
     2221 gcccaaactg acattgctca cgtgctcttt gcttgggtgg ctcgttgcca tcaccttcac
     2281 ctcggtgcca gtcagtgcga cctgtacctt tgaacccaaa ccacaacaac ctccaccgca
     2341 aggcccattt ctccataaag ttctcagcgc gaccagtacc gatggactga actggacgcc
     2401 ggataatcgg gttttgcttg accatgcgtc agtgccagcg gcgattgtca ccccaaacgg
     2461 ggcgatccga atttattatg tggatgcgag tgtgccgcca gaaacagcga acgtggctga
     2521 atcaaccgac ggcggagcaa cctttaaagt tctgggactc aaaatcaacg ggctttcatt
     2581 ccgcaaagcc ctggatccgt gcattgtccg gttgcgcgat ggacggtatc ggttgtatta
     2641 cctggcgtct gagccgggtg ataccaccac cccgcaccgc gtcaacagcg caatttcatc
     2701 tgatggagtc aactttaccg aggaacaaac cgcgttgacc tatccaggtc tggtggatcc
     2761 agatgttttt aagcgtccca aagaatggct gatgtatgtt ttttcagcca ccgacggcaa
     2821 aaccatagtc gcccgcagtc gtgacggctt gaactttacc tatctcaacc cctttgagat
     2881 tcaaggcttt ggaacaacag ccccaataaa attagctgac ggcagatacc ggctctatgc
     2941 ctttaaccag cgcggtcagc gcacctttgt cagctttatt tcaaatgacg cgctgacctg
     3001 gacacaggaa ccgggtatcc ggctggaggt cccccagggt caggaaatta cggacccgta
     3061 tgtggtccgg ctgccaaatg gcacctggaa gatgttttat aaatttcagg acaacaccca
     3121 gtcacaggca ccagccaggt attttatgag ttttcacgcc tgtgaccccg cccagggaaa
     3181 ttgtaacgat ccgcgaaccc acaaaaccta tctggcccag agcgccaatg gggaaagctg
     3241 gtcgctggtg ccaaactggc aagcctttcc gggttcagtt ccagatgtga ttcggcgtgg
     3301 caacaaactc tatgtgttca ccgccagcga tgaagtcgcc cggtataacc tcgatactca
     3361 gaccctggag ccaccggtca aagtcacggt gagtggactc agctcatttg gatttgtgga
     3421 cccgtcgctc tttgttgata accagggacg gttgatcctg ttctttctgc ggggtcaacc
     3481 cggaggcgac ccggcacagt gtccatcggg tcaaccaacg tgcgaaaaat actttgactc
     3541 agccaccgaa gttgaaggaa cagatggcac catgttcacg ctcaatgctg atcatcgcgc
     3601 cgtggttgag ctgggtgggt cagacggcct gagaaccgct tctgacccgg atattttttt
     3661 tgatggacgc cagtttgtgc tctatatctc acacggaccg agtatttcgg tctggactgc
     3721 cccaaatata ggtgatcgct tcaccaaagt cggaatgctc tcaaataata ctggggggat
     3781 tccgtcaggc tactttgatc aggaaagtgg aaggtactgg acgttcagcc acattattca
     3841 aaatgggaaa tctgtcattc ggcgggcagt ccacgctgat ttctcccgcc cactgacaga
     3901 aaccgattgg acaactgtcc tttccggcac cggattgggt ctgggcaatc aggtcaatgt
     3961 tgaaagccct gggtttgcca tcaacctcga agcacaaacc acaccgcagt gagatatcgg
     4021 gaaaagacat aaagaacaag aacactagca aaggacaaaa aggagacggg tgcggttcat
     4081 aaccaaaatg cccttcgact ttttgtcctt tttgtctctg cgttgcggaa aggttggttt
     4141 tcgcccggat gggcggtgga aaatagtcgg tgggcagggg ggcaaactca agtaaccttt
     4201 ccgcaacgga gtaaatccat caaactggtt ttcttttatc tctaaatatc tgtaagctac
     4261 tggtcagcca gttctcacgc tggtgtttga tttcccaggc c
//
LOCUS       DAOQDO010000213         4295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14027_length_4295_cov_2.975472, whole genome shotgun sequence.
ACCESSION   DAOQDO010000213 DAOQDO010000000
VERSION     DAOQDO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14027_length_4295_cov_2.975472"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKZ53_04710"
     CDS             <1..260
                     /locus_tag="PKZ53_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39769.1"
                     /translation="GVKEGDLLLGLGDVALDSPTWVTDFQKMYQGQDGKRLHIYAVRD
                     GKPLILKAVVKTIRTAEWRLVTFETTDRQDFGVRDRWLSGK"
     gene            596..1408
                     /locus_tag="PKZ53_04715"
                     /pseudo
     CDS             596..1408
                     /locus_tag="PKZ53_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1406..3355
                     /locus_tag="PKZ53_04720"
     CDS             1406..3355
                     /locus_tag="PKZ53_04720"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNJ39770.1"
                     /translation="MAGEELVRVRTDEIDSVFELSEIFDLHQSLIPATKIGDTEIIAP
                     KSLETLSIDPETGDPLWKPVSLLFRRPFEGEMVKVQLSGNKSLHTTDGHPMLVVENDK
                     LVIREAKSLASGERVPQFSGGLSPSSTSDNIEIDLIHVLPEQELSKLRVRLNNSKWET
                     HSVLLKSSFGWKIKDSIRSNSLPVKDFLAIEQNLGVQRTEITLLAGRGNSHSSFPGVL
                     SITPDFCRFLGYYLSEGCITVEKGKCRIRLTFNRKEAEFIQDVQDIMSALGITSSLYQ
                     DKNNQATTIRIGSSLLGSLLKNVLNTGVGSTSMRIPDCVMKSSVQHHEQILAGLLRGD
                     GNVEVFLNKRTYKKNGKTYTHEFNSGMVGYFSSSPKLFAQVEYLLNSMDLSPSRKANS
                     PQLRISGKENLDRMRSFFDGEKAKKLDVLSAARIRHSKQRKTLPWQGGKSVRVENIAI
                     STEKRFVYSLEVPDTHTFATTGGIFVHNCIPIDPFYLTWKAREFGVQTKFIELAGEIN
                     TAMPKYVITRLMEALSDAGKPLKHSKVMILGLAYKKNVDDPRESPSFVLWDLLLERGA
                     DVCYHDPYIPVAPYMRQHPKYAGINSLPLTPENLAAVDAVLIATSHDGIDYTEVVRHA
                     RLVVDTRNACRAVPEELKAGKLIKA"
     gene            3478..>4295
                     /locus_tag="PKZ53_04725"
     CDS             3478..>4295
                     /locus_tag="PKZ53_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017658530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39771.1"
                     /translation="MDEHREELYSDAFCPLNGSILLVDDNRVNRQILAQLLKKNGHRV
                     ETAENGKQALEIIVYQQFDLILLDILMPEIDGFQVLEQLKASPIYRHIPVLVISGLED
                     IDNIVRCIEMGAEDYLLKPFNQAILKARISACLEKKRLHDELAYTFQEVSRQRDSIEE
                     KNRQILDSIHYARRIQKAILPQPAILDHYLKNYFLIFRPKDIVSGDFFWMYVRPGHTT
                     PQVFVAVADCTGHGVPGAFMSMIGTTLLNQIVGDEGILEPAQILNRLHIGVQKIL"
BASE COUNT         1165 a          963 c         1023 g         1144 t
ORIGIN      
        1 ctggagtcaa agaaggtgac ctgctgcttg ggttgggcga tgtcgcgctt gattctccga
       61 cctgggtcac tgattttcag aaaatgtatc agggacagga tggaaaacgc ttgcatatct
      121 atgccgttcg cgatggaaaa ccgctgatcc tcaaagccgt tgtgaaaaca attcgaaccg
      181 ccgagtggcg actggtcacg tttgaaacca ccgaccggca ggattttggc gttcgggacc
      241 gctggctgag cgggaagtga taccatttag ggttcagggt atcgggttct gggttcaggg
      301 tcttcgaatt taagtccctt tcgtcccttt tgtcctttgt gtcctttttc atcatttcaa
      361 aaacccggaa cccggaaccc tgaaccctga accctgaacc tcaaacagtt ggcatcacgg
      421 gtcaggaatg gtagtctctg cccgtttttt tcaattttct ctgaaaccaa cgagttaaag
      481 cacaccaatt gcatttagaa ccgtaacctg ccagcgttgg ctcaaccagc ttttgttcaa
      541 acaagtcggc aacttcctgg cggatattct tcaatcgaaa gtgaattcat agactatgga
      601 atggaaagca aaacagaaat cagcaccggc tgtcggaatt attggtcttg ggtatgtcgg
      661 gttaccgatg gcgattcaat ttgcccgaaa tggatgcccc gtcaccgggt ttgacgtgga
      721 tgaaaaaaag accgaatcgg tcaaccttgg gaagtcatat atcaaaacca ttcagtctga
      781 agatattgcc gaacaggtca ctgccggaag gcttacggca acggctgatt tttccaaagt
      841 tgccgaccag gacgcgattc tgatttgtgt tccaacgcca cttgacgccc accgcgaacc
      901 tgacctgtcc tatgtcaccg ggaccggcaa atcaatcgcc ccgtttgttc gccgtggtca
      961 attgatcagt cttgagtcaa cgacctatcc ggggactacg gaagaagatc tgatccccat
     1021 tctggaagct ggctccgggt taaaagctgg cgaagatttt ctggttgtgt tttcaccgga
     1081 acgcgaagac cccggcaatc ccaatttcgg cacccgtgac atcccaaagg tgattggcgg
     1141 gttgaccaaa gaatgccgtg aagctggctg tttgctctat cagcgagccg tcaaaaccgt
     1201 ggtgccggtc tcgaccacac gagtcgctga agccaccaaa ctggttgaaa acattttccg
     1261 aagcgtcaac atcgctatgg tcaatgaatt aaaagtggtg tttgaacgca tgggaattga
     1321 tatttgggaa gtgctggacg ctgcccagac caaaccgttt ggatttatga aatttgagcc
     1381 tggtcccggg ctgggtggac attgtttggc tggagaagaa ttggtccgag tcagaactga
     1441 tgaaattgat tcggtatttg aacttagtga aatttttgat ctacatcaat cattgatccc
     1501 tgccacaaaa attggtgata ccgaaataat tgcgccaaaa tcacttgaaa ctctttcgat
     1561 tgatccagaa acgggagacc ctctttggaa gccggtttct ctattgtttc gccgaccctt
     1621 tgaaggtgaa atggtgaagg ttcaactgag tgggaacaaa tcacttcata caacagatgg
     1681 tcacccaatg cttgtcgtag aaaacgataa acttgtgatc cgtgaagcaa aatcccttgc
     1741 ctcaggagag agggtacctc agttttctgg cggccttagt ccatcaagta catctgacaa
     1801 tattgaaata gacctgattc atgtgcttcc tgagcaagag ctttcaaaat tacgggtgag
     1861 attaaacaac tcaaaatggg aaacacattc agtgcttctc aaatcaagct ttggttggaa
     1921 aataaaagat tccatcagga gcaattcact gccagtgaaa gactttttag cgattgaaca
     1981 gaatcttgga gtgcagagaa cagagatcac tctcttagct ggtcggggga actcacacag
     2041 ttctttccct ggtgttttaa gtattacccc agatttctgt cgatttctcg ggtattacct
     2101 cagtgaaggc tgtatcacgg tagaaaaagg caagtgccgg attcggctta ccttcaaccg
     2161 aaaggaggcg gagtttatcc aggatgttca ggatattatg tctgctctcg gcataacatc
     2221 atcgctctat caggataaaa acaatcaagc gacaaccatc agaattgggt catcgttgtt
     2281 gggatctctt ttgaaaaatg tactcaatac tggagttggc tcaacttcta tgcgaattcc
     2341 agattgtgtt atgaaatcca gcgtgcaaca tcacgaacag attcttgctg ggttacttag
     2401 aggagatgga aacgtagaag tatttcttaa taagcgcact tataagaaaa atggaaaaac
     2461 ctacacccat gaattcaatt cgggaatggt aggatatttt tcttcaagcc ctaaattatt
     2521 tgctcaggtt gagtatcttc tcaattcgat ggatttgagt ccaagccgga aagcaaactc
     2581 acctcagtta aggatttcag gaaaagaaaa tcttgacaga atgcgcagct tttttgatgg
     2641 tgagaaagcc aaaaaactgg atgttttatc tgctgctcgt atccgccact cgaaacaaag
     2701 aaaaacgcta ccctggcaag gtggaaagtc agtgagggta gaaaatattg ccatttccac
     2761 tgaaaagaga tttgtatact cactggaagt ccctgatact catacctttg ccacaacagg
     2821 ggggatattt gtccataact gtatcccgat tgatccattt tatttaacct ggaaagcccg
     2881 cgagtttgga gttcagacca aattcattga actggccggg gaaatcaaca ccgccatgcc
     2941 caaatatgtc atcacccggt tgatggaggc cctttcggat gccgggaaac cgttgaaaca
     3001 ttcaaaggtg atgattttag ggctggccta caaaaagaac gtagacgatc cacgggaatc
     3061 gccgtcattt gtgctctggg atttactgct cgaacgtggt gcggatgtct gctaccacga
     3121 cccctacatt ccagtcgccc cgtatatgcg ccagcatccg aaatatgctg gaatcaactc
     3181 gttgccgtta actcctgaga atcttgccgc tgtggatgcg gtgttgattg ccacctcaca
     3241 cgatggaatt gactataccg aagttgtgcg ccatgccaga ctggtggttg acacccgcaa
     3301 tgcctgccgg gcggtaccgg aagaactcaa ggctggaaaa ctgatcaaag cgtgaagtct
     3361 tgtgttagga aattgaaagg tgtattttcc gaaatgcacc tttcaccccc caagaaacaa
     3421 cctttgagac tgattgcgct ttgaattcat tgatacattt caactctcag gcaacttatg
     3481 gatgaacatc gggaggaatt atattctgac gccttctgcc cactgaatgg atccatcctg
     3541 ctggttgatg ataatcgtgt caaccgccag atcctggcgc aattgctcaa gaaaaatgga
     3601 catcgagttg aaacagcgga aaatggaaag caagctctgg agatcattgt ttatcaacag
     3661 tttgacttga ttttgcttga tattttgatg cctgagattg atggattcca ggtgcttgag
     3721 caattaaaag cgtcgcccat ctaccgtcac attccggtgc tggtcatttc aggacttgaa
     3781 gacattgaca atattgttcg atgtattgaa atgggggctg aagattacct gctcaagccc
     3841 tttaatcagg ctattttgaa agcccggatt tcagcctgtc ttgagaaaaa acgcctccac
     3901 gatgaactgg cctatacgtt tcaagaagtt tcccgccagc gcgatagtat tgaagaaaaa
     3961 aaccggcaga ttttagacag cattcattat gcccggcgga ttcagaaagc gattttgcct
     4021 cagccggcaa tactggatca ttacttaaaa aactattttt tgatctttcg gcccaaagat
     4081 attgtgtcgg gggatttttt ctggatgtat gttcgccctg gccataccac tccgcaggtt
     4141 tttgtcgcgg tggctgattg taccggccac ggtgttccgg gtgcgtttat gtcaatgatc
     4201 ggcacgacgt tactcaatca aattgttgga gatgaaggaa ttttagagcc agcccaaatc
     4261 ctcaatcggc ttcacattgg tgtccagaaa attct
//
LOCUS       DAOQDO010000214         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14032_length_4294_cov_3.912008, whole genome shotgun sequence.
ACCESSION   DAOQDO010000214 DAOQDO010000000
VERSION     DAOQDO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14032_length_4294_cov_3.912008"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="PKZ53_04730"
     CDS             complement(<1..1374)
                     /locus_tag="PKZ53_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNJ39772.1"
                     /translation="MLRILDRYVIREVLPYFLLSFFLLTSIIFVHEANRFSELFVVFS
                     QRGISSTPLLMLVISLLPSIVIFTIPISFLLSVMLAMGRLSGDSEIVVMTAGGVSPLQ
                     LLRPVMLVALIAGTITAYNTFYLLPVAVNSLNQLKQKRNQILVQSISTQIKPGMFAEN
                     LPNRVLYIEQSDRASDTWKNIFIAEQEEGEKDNKEPKIYTAQSGNLVLGEDLAQTELW
                     LDHSFVYASEARKFAKQKDTMSTYSKRAVIRFDLSSDNDQKKPEPPAVDAVEDLDPLP
                     PGPDLMTLPQLWRAVPEAGKERQYQIEFYKRFALPVACLFFALIGVALGITGTRSGRS
                     SGLFIGLVVNLAFYLLFLGGERAARQGVLPVLLGVWGPDLILGAVSFWLFAGGPAKFR
                     ASRLAMGFWKLWNRIQAPLRKLFRQVQQKTQDLSRSQYPLLGQGVTRRQIRLRFGFPR
                     IIDLMIFR"
     gene            complement(1555..2112)
                     /locus_tag="PKZ53_04735"
     CDS             complement(1555..2112)
                     /locus_tag="PKZ53_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ39773.1"
                     /translation="MVRTYSTMVELGTPIPAFSLPCVVTSQTISPETFADQKALLVMF
                     ICQHCPYVKHVQSELAHLGHDYAGKGLGIVAISSNDVVNYPDDAPENLKAQAVALGFT
                     FPYCYDETQEVARAFSAACTPDFFLFDESRQLVYRGQLDESRPGNGKPLNGKDLRAAI
                     DAALAGQPVPEEQTPSLGCNIKWKV"
     gene            2297..2938
                     /locus_tag="PKZ53_04740"
     CDS             2297..2938
                     /locus_tag="PKZ53_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNJ39774.1"
                     /translation="MRVRTLNHEDTHGKILYLLKTRGKLSVDDLARELDQSGVNIRRH
                     LDVMERDELVCSIVHKRERGRPTSLYFLTERGHELFPHAYDTLSVDIFEQVEQLFGRQ
                     AVRDILEGRADKLIRQLKPTLENKSFDEKVEELSHVLNSMGYMARAEHMSKTESCLLI
                     HHCPVLRIAGKYKEVCDTDCRIVRDALQARVERTCALSEGSGFCGFQVTLLPS"
     gene            3385..>4294
                     /locus_tag="PKZ53_04745"
     CDS             3385..>4294
                     /locus_tag="PKZ53_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ39775.1"
                     /translation="MSANANKAQVKLPNIKTELPGPKAKAVVEKDHRYVSPSYTRSYP
                     LVIQRGWGAIIEDVDGNHFLDFNAGVAVLATGHTHPEIVKVIADQAAEFVHIAGADFY
                     YPQLADLAEKLENETPGDFPKRVHFGNSGAEAVETALKVAAFARRRQKYIAFFNSFHG
                     RTFGALSLTASKSAQRLGFGRAALDVTHIPYANCLRCAYGKQPETCNVECAKVIEDRL
                     FKTTVPADEVAAIVVEPVQGEGGYVVPPSKFLKELRRIADENNIVLIVDEVQSGMGRT
                     GKLFAIEHHDVVPDVICSAKGIASGLP"
BASE COUNT         1089 a         1144 c         1026 g         1035 t
ORIGIN      
        1 gcgaaaaatc atcaggtcga taattcgcgg aaatccaaat cgaagccgaa tctggcgccg
       61 ggtaaccccc tggccaagca acggatactg tgatcggctc aaatcctggg ttttttgttg
      121 aacctgacga aagagtttgc gcagtggagc ttgaatccgg ttccacagtt tccagaaccc
      181 cattgccagc cgtgaagccc ggaatttggc tggtccaccg gcaaaaagcc agaaactgac
      241 cgcacccagg atcaaatcgg ggccccacac accaagcaat accggcaaca ccccctgtcg
      301 ggcggcgcgt tccccaccca aaaacaacaa gtagaaggca aggttgacca ccaatccaat
      361 aaacagccct gatgaccgtc ccgaacgcgt tccggtaatc cccagcgcca caccaatcaa
      421 agcaaagaag agacaggcaa ctggcaacgc aaatcgtttg tagaattcaa tctggtactg
      481 acgttctttt ccggcctcag gcacggctcg ccagagttga ggtagcgtca tcaggtctgg
      541 ccctggtggg agggggtcta aatcttcaac cgcatcaacc gcaggaggtt cgggcttttt
      601 ctggtcatta tcgctgctca aatcaaaccg aatcaccgcc cgtttggagt aagttgacat
      661 cgtgtctttc tgtttggcga actttcgggc ttctgacgca tacacaaaac tatggtccag
      721 ccataattcc gtctgggcca gatcttcgcc aagaaccagg ttaccgcttt gggcagtgta
      781 gattttcggt tctttattgt ctttttcgcc ttcctcctgc tcagcaataa aaatattttt
      841 ccaggtgtcg ctggcccggt cgctctgttc gatatagagc acccgattgg gcaggttttc
      901 agcaaacatc cctggcttaa tctgggttga aatactctga accagaatct ggttgcgctt
      961 ttgctttagc tggttgagcg aattgaccgc caccggcagc aaataaaagg tgttataggc
     1021 agtaatggtg ccggcaatca gtgccaccaa catcactggg cgcaacaatt gcaagggact
     1081 cacgccgcca gccgtcatca caacaatttc actatcgcct gacaaccgcc ccattgccag
     1141 catgacactg agcaaaaatg aaatgggaat cgtgaaaatg acaatgctgg gcaggagtga
     1201 aataaccagc atcagcaatg gagtactgga aattccccgt tgtgaaaaaa cgacgaataa
     1261 ttcagaaaag cgattggctt cgtggacaaa aataatcgag gtcagcagga aaaagctgag
     1321 caggaaatat ggcaggactt cacggatgac gtaccgatca agaatacgga gcatggcaaa
     1381 ccactggaac acaaaacagc taaaatcgaa gacggggact ttaccctaga ggatctgtgt
     1441 gaaacaagtg tgaacacttt cattcgggct gaagaaaacg gggttcatcc cctgagcccg
     1501 acttcttcag ccctgagccc aatgtcttca acccgatgtc ttcagcccca aattttagac
     1561 cttccacttg atgttgcacc ccaggcttgg ggtttgttct tccggaactg gttgcccagc
     1621 cagggcagca tcaattgcag cccgtaaatc ttttccattc aatggttttc cgtttcctgg
     1681 acgactttca tccagttgtc cacggtagac aagctgtcgg ctttcatcaa ataagaagaa
     1741 atccggtgtg caggcggctg aaaaagcacg ggcgacttcc tgggtttcgt catagcaata
     1801 ggggaaggta aacccgagcg cgacggcctg cgccttgagg ttttcaggcg catcgtcagg
     1861 gtagttcact acatcatttg aactgatcgc cacgatccca agacctttcc cagcataatc
     1921 atggccaagg tgtgccagtt cggactggac gtgtttgaca tagggacaat gctggcaaat
     1981 aaacatcacc agcaaagctt tctgatcggc aaaggtttca ggagaaatgg tttggctggt
     2041 tacgacacag gggagtgaaa aagctggaat gggggttccc agttcaacca tagttgaata
     2101 tgtgcggacc atcagtgaca tcctttctgg tttcaaaatg agagctggct taacttagat
     2161 tatgaaacga ttatctcctt gaatcataac cttttagata atctttcatt cttcattctt
     2221 cattcttcat tcgatgtaag gagccggaga tgcgggttct ggctcgatga gtgcatgtgg
     2281 tacagttacg cccatcatgc gcgtaagaac tctgaatcac gaagatactc acggcaaaat
     2341 cctctacctg ttaaaaactc gcggcaaact gagcgttgat gacctggcac gtgaactcga
     2401 tcaatcaggt gtcaatattc gtcggcattt ggacgtgatg gaacgggatg aactggtttg
     2461 ttcaatcgtt cataaacgtg aacgcggtcg tccaaccagc ctctactttt tgaccgagcg
     2521 cggccatgaa ctcttcccgc atgcgtacga caccctcagt gtagatatct ttgaacaggt
     2581 tgaacaattg tttggccgtc aggccgtccg ggatattctg gaaggccggg ccgacaagtt
     2641 gatccggcag ctcaaaccga cactggaaaa taaatcattt gatgaaaaag tcgaggaact
     2701 ttcgcatgtc ctcaacagca tgggctatat ggctcgtgct gagcatatgt ctaaaaccga
     2761 atcctgtctg ttgattcatc actgcccggt gcttcgaatc gcaggtaaat ataaggaagt
     2821 gtgcgatacc gattgtcgga tcgttcgcga cgcccttcag gctcgggtcg agcggacatg
     2881 tgccctgtct gaagggtcag gattttgcgg gtttcaggtc accttgctgc cttcgtaaac
     2941 aagccttgcc tgccaaagca gcctcttgct ttttgcacgg taaagctagt acactcagct
     3001 tcttatccca gataagaatg cttcgcttga atcatcgtaa caacttccct tctcaccctt
     3061 tctgccgctg aagttgttgc cagttcacac ctcaatctca aaaccactca tttggtttgt
     3121 gtttttgccg atcatgcata tttttgctga cgcccactcc aaccatctcc gtgtcgccgt
     3181 gcaactggcc ccgatccagc aacggcgcgg cctgttcggg gattaatcct cctgcccacc
     3241 aatttgccct tcgcttttat tcgctaacct ttttttcagg ccatactctg gcctgttccg
     3301 aattgaatcc ctgattcatc cacactggca gtgtgacggg gaaacagggt tcactgtctt
     3361 tttatccaac ggaggttaat tctcatgtca gccaatgcca acaaagctca agtcaaactc
     3421 cccaacatta aaaccgaact ccctggccca aaagccaaag cggtggttga aaaagatcat
     3481 cgctatgtgt cgcccagcta tacccgaagc tacccgctgg tcattcagcg tggctggggc
     3541 gccattatcg aagacgtaga cggcaaccat tttctcgatt tcaatgccgg cgtggcagtg
     3601 ctggcaacag gtcataccca tccagaaatc gtcaaagtga ttgctgatca ggccgctgaa
     3661 tttgtccaca ttgccggcgc tgatttttat tacccacaac tggccgacct ggccgaaaag
     3721 ctcgaaaatg aaacgccagg tgatttcccc aaacgcgtcc actttggcaa ttccggggct
     3781 gaagccgttg aaacagcgct caaggtcgcg gcctttgccc gccgccgcca aaaatacatt
     3841 gctttcttca atagcttcca tggccgcaca tttggcgcct tgtccttgac cgccagcaaa
     3901 tcagcccaac gactcgggtt tggccgtgca gccctcgatg tgacgcatat tccatatgcc
     3961 aactgtctgc gctgtgccta tggcaagcaa ccggaaacct gcaatgtgga atgcgccaag
     4021 gtgatcgaag accgtctctt caaaaccacc gtgccagccg acgaagtcgc cgccattgtg
     4081 gttgaaccag ttcagggtga aggcggatat gtcgtgccac catcaaaatt cctcaaagaa
     4141 ctgcgtcgca tcgctgacga aaacaacatc gtgctcatcg ttgacgaagt ccaatccgga
     4201 atgggtcgta ctggcaaact ctttgccatt gaacatcacg atgtggtccc cgatgtcatt
     4261 tgcagcgcca aaggtattgc ctcaggtctc ccgt
//
LOCUS       DAOQDO010000215         4291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14049_length_4291_cov_3.720255, whole genome shotgun sequence.
ACCESSION   DAOQDO010000215 DAOQDO010000000
VERSION     DAOQDO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14049_length_4291_cov_3.720255"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKZ53_04750"
     CDS             <1..336
                     /locus_tag="PKZ53_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ39776.1"
                     /translation="VNYSMGMKRKLGLACAQIHDPDVYILDEPTNGLDPIAQREVQNW
                     ITSSAQAGKTIFLSTHLLDMAEKLCHRVAIIHQGEILTVGSPQQLKNQLSAGGSLEEV
                     FFAVTRAED"
     gene            458..2836
                     /locus_tag="PKZ53_04755"
     CDS             458..2836
                     /locus_tag="PKZ53_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNJ39777.1"
                     /translation="MSPRLSAFQIFCVVSKYKFLQLRNILAATLNPQRKGTQSDASRQ
                     GTARNGQMSVSRVISSLVLIWIFFGGMSLITMSGLVTNFKKNPQLIGFKLPTTGQQLR
                     LDPGISEAGKSIVRSRPFLHQVSFLALITFVGLIAASMGFHGRNLARLDTVVEQLLTL
                     PISIPLIFYSRLVEYIVFGFGWGVYFPFCTVLLFVLGYRWSVIPLALGLTLIFNCVLA
                     IVQFVLEIFLRRFFSASGISNVQILATLSGSLFVSVFGMFPIFGEFYPTMFQWCDRIG
                     RVAWYLVPSLGFNVLQGGVSASDFGALGIQLAGVMVTGLMGWTIIGWASRAGLEAVSG
                     REAGQRKQELKLSPGRIPGFFRRELIILRREKRAWISMLSPLLMLLPILIIPNVASSI
                     LSDPLKSGAVAFGYGMLLLVTVSISIVIFEGEAFWILYTVPQSLLRLLLQRTVFWLIL
                     AWVTSLVIWMSGIVYRHHFETGDLVGVVWIVVGLPIMGLLGMAMGMLFTDPLATESNQ
                     QIRSDLAILPLAFGGLFAGGMFLPGIWLKLNCLFLFGMLAASWLQKASQQVDYLLDPT
                     ALPPRKIHAADGFSIGVLFLVILIFLTAGFTFPAAMKPQVAFSVSYCVAALVTVCAMA
                     AYASSEQLPLKETFSLWQSGIEESRETVLVTLKGIGLVLVIVGGSYFLPVPRPPLPAG
                     PAGVGERLVLTILGPVIALGIKPVWEELLFRGFIYQGLKTSVSPMWAGILSACVFCLF
                     QPPWNAIPVFLLGLVTAWTFERSKSLIASMVVHAVYNGGLIGIQWLVYTKYR"
     gene            complement(2981..3703)
                     /locus_tag="PKZ53_04760"
     CDS             complement(2981..3703)
                     /locus_tag="PKZ53_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNJ39778.1"
                     /translation="MKSIALLFSSILVSVLLFAAPMTKAATPQQDAENRVFTTLEEAL
                     AQPEAVYNLDLRSKQLVSLPPEIGNFPNLRRLNLAGNSLTALPPEIGKLTKLEWLSLA
                     SNELATLPVEIGNLKHLQELYLAANQLTAVPVEIGNLTSLQTLGLSVNQLTVLPNEIE
                     KLTMLVTLRVNSNLLTAIPPGIGKLKKLENLQVNNNQLTDLPIEIKNLPKLKAMSYEN
                     NQFSPEKQIEIDKLYGPFQVFK"
BASE COUNT          915 a          964 c         1157 g         1255 t
ORIGIN      
        1 gtcaattact caatggggat gaaacgcaag ctgggattgg cctgcgcgca gattcacgac
       61 cctgacgtgt atatcctgga tgagccaacc aacggtctgg atccaattgc ccagcgcgaa
      121 gtccaaaact ggatcacaag ctctgctcaa gctgggaaaa ccatttttct ttccacccac
      181 ttgctcgata tggccgaaaa gttgtgtcat cgggtggcga ttattcacca gggtgaaatc
      241 ctgacggttg ggtcacctca gcaactcaaa aatcagcttt cggcaggtgg ttcacttgaa
      301 gaggtctttt ttgccgtcac cagagctgaa gactgatgag aacaggggct tggggctgaa
      361 gccgttgggc ttggggctgg aacaagcact tttcttcagc ccaacttttt cagccctcaa
      421 ccctcagccc aatgtcttca gcccaaaggt ttcatctatg tcccctcgtc tttctgcctt
      481 tcagattttc tgcgttgttt caaagtacaa gtttctccaa ctgcgaaata ttctggccgc
      541 taccctcaat ccccagcgaa agggaactca gtctgacgca agtcgtcagg ggacagctcg
      601 caatgggcag atgtcagtct cacgggtaat ttcaagcctg gtgctaatct ggattttctt
      661 cggtgggatg tcattgataa cgatgagtgg tctggtgacc aacttcaaaa aaaatcccca
      721 gttaataggc tttaaacttc caactactgg ccagcagctc agacttgatc cagggatttc
      781 tgaagccggg aaaagcattg tgcgatcccg tccgtttcta catcaggtct cattccttgc
      841 cttgataact tttgtcggac ttattgccgc gtcaatgggg tttcatggtc gcaatctggc
      901 ccggcttgat acggtggttg agcaattgct cacattgcca atttcaatcc cgctcatttt
      961 ttatagccga ctggtcgaat acattgtgtt tggttttggt tggggggtgt atttcccatt
     1021 ttgtaccgtt ttgctctttg ttttgggata tcggtggagc gtgattccgc tcgcccttgg
     1081 cctgacgctg attttcaact gtgttttggc gattgtgcaa tttgtccttg aaatcttcct
     1141 gcgacggttt ttttcggctt ctggcatttc caatgttcaa atcctggcca cactcagcgg
     1201 aagtctcttt gtttcggtgt ttgggatgtt tccgatcttt ggggagtttt acccaactat
     1261 gtttcagtgg tgtgatcgga ttggccgggt cgcctggtat ttggtgcctt ctcttgggtt
     1321 caatgtgctg cagggaggtg tatctgccag cgatttcggt gctctgggca ttcaactggc
     1381 tggtgtgatg gtgactgggt tgatggggtg gacgatcatt gggtgggcgt cacgcgctgg
     1441 actcgaagcc gtctctggtc gggaagctgg tcagcgaaag caagaattaa aactttcgcc
     1501 aggtcgcatt ccaggattct tccgccgcga gttgattatc cttcggcgtg aaaaacgagc
     1561 ctggatatca atgctctcac cactgttgat gctgctgccg atacttatta ttccgaacgt
     1621 ggcgagcagt attctttccg acccgctcaa atcaggcgca gtggcttttg gatatggaat
     1681 gctgctgctg gtgacagtgt caatcagcat cgtaattttt gaaggcgaag ccttttggat
     1741 cctctacacc gtgccgcagt cactgcttcg gctcttgctc caacggaccg ttttttggct
     1801 catcctggcc tgggtaacgt cgctggtcat ctggatgagt ggcatcgtct atcgccacca
     1861 ctttgaaacc ggtgatctgg tgggagtggt atggattgtg gttggtttac cgatcatggg
     1921 cctgttgggg atggcaatgg gaatgctctt taccgatccg ctggccacag agtccaatca
     1981 gcaaattcgg tcagatctgg caatcttacc gctggcgttt ggaggattat ttgctggcgg
     2041 catgtttttg ccaggtatct ggttgaagct aaactgcctg tttttgtttg gaatgctggc
     2101 agcgagctgg ttgcagaagg ccagtcagca ggttgactat ttgcttgatc caacggcgtt
     2161 gccaccccgg aaaatccatg ccgctgatgg gttttccatc ggggttttat ttctggtaat
     2221 cctcattttt ctgacagccg gctttacctt tcccgccgca atgaagccgc aggtggcctt
     2281 ttccgtgagc tattgtgtcg ctgccctggt aacggtgtgt gccatggcag cctatgcgtc
     2341 aagcgaacaa cttccgctca aagaaacctt ctcgttgtgg cagagtggaa tcgaagaatc
     2401 tcgcgagacg gttttggtga cgctcaaagg gattggcctg gtgctggtga ttgttggagg
     2461 aagttatttt ttgccggttc caagaccgcc gctgccagcc gggccagccg gtgttggtga
     2521 aagactggtt ctgaccattc tcggaccggt gattgccctg ggcatcaaac ccgtttggga
     2581 agaactcttg tttcgcgggt ttatctatca agggttgaag acgtcggtga gtccgatgtg
     2641 ggcgggaatt ctatctgcct gtgtcttttg cctgtttcag ccgccctgga atgccattcc
     2701 ggtgtttttg ctgggactgg tgacggcctg gacgtttgaa cggtcaaaaa gcctgatcgc
     2761 ttcgatggtt gtgcacgcgg tttataatgg cggattgatt ggaattcagt ggcttgtcta
     2821 tacgaagtac cggtaacgag gcgaaaacga aaaaagcggt gtcaagccac cgcactccga
     2881 aaaagaaaaa actggcggtt ggtcaccgcc agttttttgg ccgttttggg tagagaaagg
     2941 gaacggtgat gtggagtcaa ccggttaccc tttcaaacgg tcacttgaac acctgaaatg
     3001 gtccatagag tttgtcaatt tcaatctgtt tctcgggtga aaactgattg ttttcatagc
     3061 tcatggcttt caatttgggc aggttcttga tttcaattgg caggtcggtc aactgattgt
     3121 tattgacctg gaggttttct aatttcttca acttgccaat gcctggcgga atcgccgtga
     3181 gcaggttgct attcacccgg agcgtaacca gcatagtgag tttttcaatt tcattgggca
     3241 ggacggtcag ttggttgact gaaagtccca atgtctgcag gctggtcaaa ttcccaattt
     3301 cgactgggac tgccgtgagt tgattggcgg ccagatacag ttcctgcaaa tgttttaaat
     3361 ttccaatttc aaccggtaac gtggccagtt cattgctggc gaggctgagc cattcaagtt
     3421 ttgtcaattt tccgatttcc ggcggtagtg ctgttaacga attgccagcc agatttaatc
     3481 ggcgaaggtt tgggaagttt ccaatttcag gtggaagaga aacaagttgt ttgcttcgga
     3541 gatccagatt gtacaccgct tcgggttgtg ccagggcctc ttcgagggtg gtgaaaactc
     3601 tgttttcagc gtcctgttgg ggggtggcag ctttggtcat tggagcggcg aatagcagaa
     3661 ctgaaaccag aatgcttgaa aacagcaacg cgatggattt cataattccc cctctcaacg
     3721 taaagcttgc cggtcgtggg taggccaaac aagtaaaaga aacttttgtt gatgtgtact
     3781 gattgcactg cttgaggaga gcaagaatta tgccatggtt ttagccagac tgatatgaaa
     3841 ataactatat gttttgcctt gttttgaaaa gtatataaat tgttttattt aaatagttta
     3901 tggtgtgtgc ctgatgaggg aatttcaatt gcctgatgat ggctgttggg gagggcttcg
     3961 cgagagggaa aattaccagg aaaatgccca aagaattggg gaaagatacg cgggaaattc
     4021 aacccgtcat tgcggaaaat ttccggatat ttatcctctc tcttctggaa tggtgctcat
     4081 gtcgtgcccg agattctgga ttgtttcttt ggcttggcaa ggctataacc agttgatcaa
     4141 accaactcaa tacacatccc cgcccagtgg ttgattgccc aacggtgtgg atttgccttc
     4201 cttccattct gatcctggtg aaaccacgat gaaaaacatc cgaaccttgc tgagcagcgc
     4261 ctgttgtctt ggattgagtc tggggctgac c
//
LOCUS       DAOQDO010000216         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14056_length_4290_cov_3.181582, whole genome shotgun sequence.
ACCESSION   DAOQDO010000216 DAOQDO010000000
VERSION     DAOQDO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_4290_cov_3.181582"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1917
                     /locus_tag="PKZ53_04765"
     CDS             130..1917
                     /locus_tag="PKZ53_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNJ39779.1"
                     /translation="MYFLFAGIGMLLLVAVIGWVTQKPAHSEWSERPQDRRDLAQLTT
                     STGLDIYPAVSPDGNSIAYSSDRSGKFEIYVRPLTPGGREIQITNDGEQNFQPAWSPD
                     GKMIAYFSQNRHGIYLIPALGGVAKQLTEFGSQPDWSPDGKTIVFQEDELSDLAPVAL
                     SALPPSTLWTIPAQGGAPRQITKIQKPSGGHGAPHWSPDGKWIVFIAYEFVYNLGDAG
                     IWVVSPETGVMNRLSWNPHLTFSPIFSPDGKAIYYVGMSSSKNLYLWKIDFNSETGKP
                     NGKPVEVTNTGYSLIKYLSMSPDGKRIYYTALNMTNNLWSVPVSSTTGEATGEPHALT
                     SDTSFRKTLPAFSADGRKIAYDVRVAGARPDVWIMDADGKNQTQVTTDEHNDSLLGWL
                     PDNDQVVFVSDRNREMFFWMASTRRGIEKPLTQFTHPGGHGRLSPDGKFIAYDVPFDN
                     AVNVWVAALDGSGLRQVTTGPGFTAFPAWSSDGKWLAVEIKDRGFCNVGIVPIEGGPV
                     TQLTFEQGQNWPYTWSPDSSKIALAKYKDGFWNVWWISPTTREQKQITSFARPNCYIR
                     YPAWSPKGDQIVFEHAEITGNIWSMTVGE"
     gene            complement(2078..3061)
                     /locus_tag="PKZ53_04770"
     CDS             complement(2078..3061)
                     /locus_tag="PKZ53_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39780.1"
                     /translation="MSIFEPKLSEIDPVAARFNEAVNDAWFVRTFGALALGASVASMA
                     SIGFLIGAGMAVMGFGTSRFYRWLGLAIILLSGTGMFLPPGLQSLGHCASVLALGGGV
                     CVKGFKILELLGGSDQSDPDWKPTQRKALVGMLLSGLGVILVLGWVTLSVTGYLFFPK
                     PNQRSQQQVGTVGLKRFQLGKAALHIDLPGQPSLQTDLVQENVKNQIDHNQIYQWNQD
                     GILVHIESVVFHTGIQPDIQAIASAANQNLRSTNEKDAPPQIQASNDPTNPSRLFLTG
                     TMMLNGKNLELVGVVLNRNENVAVVVALYDPQNQSSYRLAQQVLDSASLGS"
     gene            complement(3271..>4290)
                     /gene="ribD"
                     /locus_tag="PKZ53_04775"
     CDS             complement(3271..>4290)
                     /gene="ribD"
                     /locus_tag="PKZ53_04775"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNJ39781.1"
                     /translation="QIIGRGCHRFEERRHAEVNALEEAGELARGATMYTNLEPCAHFG
                     RTPPCTQAIIQAGITRVVSAMRDPNPQVFGKGFEQLEAAGIEVLVGIGERESRHLNEV
                     FVTYHTKGRPFIHLKTAMSLDGRTATRTGDAQWITGEAARIEVQKLRYGYDAVMVGIV
                     TAIADDPALTYRGNLPRHQPVKRLILDSELRLPLTSSLVQTAFEAPVWVFTDEEGDKR
                     ERQRQLEAHGVRVFHVPSFFDPETQRPRLDLPAVLQSLADHHVTSVLVEGGAEVAGSF
                     LTSMLIDKVTFFIAPLLIGGRLATSAVAGDGFARLDESLRLVDVEVKHVGNDVMITGY
                     PLHAI"
BASE COUNT         1067 a         1114 c         1083 g         1026 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccgctgcc tttgacctat cccccatccc ccatccccca
       61 tcccctgttt ctatccctga cccccgaccc ctgacccctg actcgtataa ttcccgtctc
      121 catttcccga tgtattttct ttttgctggg atcggaatgt tgctgctggt ggctgtgatt
      181 ggatgggtaa cccagaaacc tgcccatagt gagtggtcag aaaggccgca ggatcggcgt
      241 gatctggccc aactgacaac atcaactggg cttgatattt atccggcggt ttctccggat
      301 ggaaactcaa ttgcctatag ctcggatcgg agcgggaaat ttgaaatcta tgtccggcca
      361 ttaacgccag gtggtcgcga aatccagata accaatgacg gcgagcagaa ctttcaacca
      421 gcctggtcac ctgatggaaa gatgattgcc tatttctcac aaaatcgtca cggtatttac
      481 ctcattccag ccctgggtgg cgtggccaag cagctcacag aatttggatc acagcctgat
      541 tggtcacctg acggaaaaac aattgttttt caagaagatg aactctctga tttggcgcca
      601 gttgccctca gtgcgctacc accttcgaca ttgtggacga ttccagcaca aggtggtgcg
      661 ccacgccaga tcacaaaaat tcaaaaacca tcaggtggac atggggcccc tcactggtct
      721 cccgatggca aatggatcgt gtttattgcc tatgaatttg tttataacct gggagatgcc
      781 gggatctggg ttgtgtcacc tgaaacaggc gtgatgaatc ggctttcctg gaatccacat
      841 ctgaccttca gtccgatttt ttcgcctgat gggaaagcca tttattatgt gggaatgtct
      901 tcgtccaaga atttgtacct gtggaaaatt gattttaact ctgagactgg aaaaccaaat
      961 gggaagccag ttgaggtaac caatacgggc tattcattga tcaagtacct gtcaatgtcg
     1021 ccggatggca aacggatcta ttacacggcc ttgaatatga ccaacaatct gtggtcagtt
     1081 ccggtctcgt caaccaccgg agaagcaacc ggcgagcccc atgccttgac cagcgacacc
     1141 agtttccgca aaacgctgcc agctttctcg gctgatggcc gcaagattgc ctatgatgtt
     1201 cgggtcgctg gtgccagacc tgatgtctgg attatggatg cggatgggaa aaaccagacc
     1261 caggtaacca ctgacgagca caatgattct ttgctcggat ggctgccgga caatgatcag
     1321 gtcgtttttg tatctgatcg aaaccgggaa atgttttttt ggatggcttc gacccggcgt
     1381 gggatagaaa aaccattgac ccagtttacc cacccaggtg gacacgggcg gttgtcaccg
     1441 gatgggaagt tcattgccta tgatgtgcct tttgataatg ccgtcaatgt ctgggttgcc
     1501 gcactcgatg gcagcggact acggcaagta accactggtc ctggatttac ggcctttcct
     1561 gcctggtcat ctgatggtaa atggctggcg gttgaaatca aagatcgtgg tttttgcaac
     1621 gttgggattg ttccaatcga aggtggtcca gtcacccagt tgacctttga acagggccaa
     1681 aactggccat acacctggtc tccggacagt tcaaaaattg cactggccaa gtacaaagac
     1741 ggcttctgga atgtgtggtg gatttcacca accacacggg aacaaaaaca aatcacgagc
     1801 tttgccaggc caaattgtta tatccgctat ccggcctggt ctcccaaagg cgatcaaatt
     1861 gtcttcgaac acgccgaaat caccggcaat atctggtcaa tgacggtggg agaatgacca
     1921 gcaggactga agaaaatcgg gctgaagaat gcgggctcag ggctgaagat tcgcaagctc
     1981 agggctgaag acactgggca gagaagcgaa ataattggaa aatatgtgtt tccctcggcc
     2041 ctgagcccaa aatggcagta cccattccta cctcccacta tgagccaagg gaagctgaat
     2101 caagaacctg ttgggccagg cggtaggatg attgattctg aggatcgtac agcgcaacca
     2161 caacggcgac gttttcattc cgattgagca ccaccccaac cagttcaagg tttttcccat
     2221 tgagcatcat tgttccagtc aagaacaggc gggaaggatt ggttgggtcg ttgctagcct
     2281 gaatctgggg cggagcatct ttttcatttg tgcttcggag attttggttg gcagcggagg
     2341 cgattgcctg aatatcaggt tgaatgcccg tgtgaaacac gacgctctca atatggacca
     2401 aaattccatc ctgattccat tgataaatct gattatggtc aatctggttt ttgacattct
     2461 cctgaactaa atctgtttgg agagacggct gacctggaag atcaatatgt agcgccgctt
     2521 tgccaagttg aaatcgtttt aaaccaaccg ttcctacctg ctgttgagac cgttggtttg
     2581 gtttcgggaa aaacaagtat ccagtgactg aaagcgtaac ccatcccaac acaagaatta
     2641 cccctaaccc gcttaatagc attccaacca gagcttttcg ttgagttggt ttccaatctg
     2701 gatcactttg gtcagaaccc ccgagcaact ccagaatttt aaatcctttg acacacacac
     2761 cgcctccaag tgcaagaact gaagcacagt gtccaaggga ttgcagccct ggtggcaaga
     2821 acatccccgt acctgacagg agaataatcg caagtccaag ccatcggtaa aaacggctgg
     2881 ttccaaagcc catcacggcc attcctgccc caattagaaa tccaattgag gccatgctgg
     2941 ctacagaagc tcccagggcc agggcgccaa atgttcgaac aaaccaggca tcattgacgg
     3001 cctcgttaaa ccgtgctgca accgggtcaa tttcagataa tttcggttca aaaatactca
     3061 taaggattgt aaataaagta ggtacacgac tattttggtg acgggtgatg ggtgcataac
     3121 accacatggc aatgacatca ctgccagttc agggaacgaa aaccagcccg caaagatttg
     3181 atttggattg accaaatccg tcgccttcag ccttgagctt gcgaatcttc agcccaatat
     3241 tcttcagccc gctttcttca gccccaacct tcagattgcg tgcagcgggt agcccgtaat
     3301 catcacatca ttgccgacat gtttgacttc gacatccact aatcggagtg actcatccag
     3361 acgggcaaaa ccgtctccgg ccacagcact ggtggccaaa cgtccgccaa ttaacagcgg
     3421 cgcaataaaa aaggtcactt tatcaatcaa catcgaggtc agaaacgagc cggcgacttc
     3481 ggcaccgcct tcaaccagca cactggtgac gtggtggtca gccagacttt gaaggactgc
     3541 aggcaaatcc aatcgtgggc gctgggtttc cgggtcgaaa aacgatggaa cgtgaaagac
     3601 tcgcacaccg tgcgcttcaa gttgtcgctg gcgttcccgt ttatctccct cttcgtcggt
     3661 aaagacccac accggcgcct caaacgcggt ttggaccagg gacgaggtga gtggcagtcg
     3721 tagttcagaa tccagaatga ggcgtttgac tggttgatga cggggcaagt ttccgcgata
     3781 ggtgagcgcc gggtcatcgg caatggccgt cacaatgcca accataacgg catcatatcc
     3841 atagcggagt ttttgaactt cgatgcgggc ggcttcgccg gtaatccatt gcgcatctcc
     3901 ggttcgggta gcagtccggc catccagact catggccgtt ttcaaatgga tgaaaggccg
     3961 acctttggtg tgataggtca caaacacttc gttgagatgt cggctttcgc gttcaccaat
     4021 accgacgagg acttcaatcc ccgcggcttc gagttgttca aatccttttc caaatacctg
     4081 tgggtttggg tcgcgcatcg cagaaacaac tcgggtaatt cccgcctgaa taatcgcctg
     4141 ggtgcagggt ggggtgcggc caaagtgggc acagggttca aggttggtgt acatcgtggc
     4201 cccccgcgcc agttcaccgg cttcttcgag ggcgttgact tcggcgtggc ggcgttcttc
     4261 aaaacgatga caccctcgcc cgatgatctg
//
LOCUS       DAOQDO010000217         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14091_length_4282_cov_2.702626, whole genome shotgun sequence.
ACCESSION   DAOQDO010000217 DAOQDO010000000
VERSION     DAOQDO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14091_length_4282_cov_2.702626"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4206
                     /locus_tag="PKZ53_04780"
     CDS             <1..4206
                     /locus_tag="PKZ53_04780"
                     /inference="COORDINATES: protein motif:HMM:NF040133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39782.1"
                     /translation="GPTAGGTIELGKSVSTACVSGWVTLNPSSQLLADAVTDGNGGNV
                     QVWSEKETICTALISAQGGKQGGKGGLVEVSSRNGVSLSGEVNVKAQSGTNGKFLLDP
                     KNIVVDNATGVFPQFTLVDPNPGGTSASLFVQALSNGNLVVSKPGDNFAASNSGAVYL
                     YNGLTGALISALRGSSANDQVGNGVAQPLSNGNFVIRSSSWANGGAATWGSASTGVNG
                     AVSAANSLVGSTATDRIGIFVTSLTNGNYVVGSPFWDNGSVVDAGAAAWGNGTTGTSG
                     VLSAANSLVGSSTGDQVSSRSIFALTNGNYVVNSQLWNNGAASQAGAATWGNGTTGVT
                     GVVSASNSLVGSTTGDQIGSSFTFALPNGNYVVASPNWANGSALQAGAATWGNGTTGI
                     TGPVSAANSLVGGQMGDQVGILTTALRNGNYVVSSSRWNNGSTVDVGAVTLGNGTTGT
                     TGLVTASNSLVGSSPGDSVGSNFILELTNGNFITRTSSWRNGSATNAGAVTLVNGVTG
                     LTGPVTPSNSLVGTTMNDGVGFTPAIALTNGNYVVASPLWNNGSATDAGAITWGNGTT
                     GVTGPVTPSNSLVGTTTDDRIAFGTLVALTNGNFVVCSPDWDNGGTVDAGAVTWGNGT
                     SGITGPITPANSLVGSTTNDHVGNLTPLILTNGNYVVCSRLWDNGSIVDAGAATWANG
                     TTGISGPVTTSNSLFGTTANDQVGNAFVQSLTNGNYTVVSLQWDNGSAVDAGAVTWGN
                     GTTGITGPVSPANSVVGSTTNDQIGFGSIIALSNGNYVIQGPGWDNGSTVNAGAAIWV
                     NGSTGQTLNGLNTISAQNSIIGSVPNAAGSFFGFFEDTVNDSFLVSFSNDPPSGRITV
                     GVPNPNQLTYNRGQASTMTITADFLKRTLDAGTDVVLQANNDITINSPLSIAAAGGDL
                     TLQAGRSITVTSTLSAINSDLNLLANQPVSTGVVNAQRDAGAAVITASAPINLGTGRF
                     TARIDTGSGLTNQTNGAITLSTVTANRITITNNGPTAGTGRVRVTGTITGGACNGASL
                     CTASGGGGQVTINHPNSGVDFLIGDATTNGTAGTIVTGTVTVAAPFTVMNVPGTFSQG
                     NVSISPGQTLIPPGLQALLMVADTINNRVQQFDGTTWSVIGVGTVGSGDGQFRQPEAV
                     TFDSTGRIFVADTGNDRIQFSTDSGVTWANFATVGSGLNQVRAPQGLALDSDGNLYVS
                     DTGNGRVIRFNGGIPGSGIVIATNGAGGGQVGSPRGLTIDTTFRLFVADESNSRILRI
                     LNANTTTSGTSGTIIASQGTALNKVKNPQGVTVDLTTGTLFIADTGNSRILSFPNGNA
                     NNASAIALTGSQLGQVNKAEGVTVSFFTAGPFAGNVLLVVGDTFNNRIQGRFLPNGAW
                     SLIGTPNNIGSGVGQFRNPSKIR"
BASE COUNT         1005 a         1094 c         1227 g          956 t
ORIGIN      
        1 ggccccaccg ccggtggaac catcgaactc ggaaaatcag tcagtaccgc ctgcgtcagc
       61 gggtgggtga cgcttaatcc ttccagccaa ttactggccg atgccgtgac tgatggaaat
      121 ggtggaaacg tgcaggtgtg gtctgaaaaa gaaaccatct gcacggcatt aatcagtgcc
      181 cagggtggaa agcaaggcgg aaagggtggc ctggttgaag tttcaagccg aaacggtgtt
      241 tccctgagtg gtgaggtgaa cgtcaaagcc cagtcgggca caaatggcaa gttcctgctg
      301 gatcccaaaa acattgtggt ggataacgca actggtgtct ttccacaatt tacactggtt
      361 gatccaaatc ctggcggcac aagtgccagt ctgtttgttc aggctctgag caatggaaat
      421 cttgtggtga gcaaacccgg tgataatttt gccgccagca actcgggggc ggtgtatctc
      481 tacaatggat tgaccggcgc cttgatcagc gcactgcggg gcagtagcgc caatgatcag
      541 gtcggcaatg gggttgccca acccctgagc aatgggaatt ttgtcattcg gagttcttcc
      601 tgggcgaatg gcggtgcggc cacctggggg agtgccagca cgggtgtcaa cggtgctgtc
      661 tcagccgcca atagtcttgt cggaagtacg gctactgatc ggatcggtat ttttgtcaca
      721 tccctgacaa atggaaatta tgtggtcggc tctccatttt gggacaacgg ctcggtggta
      781 gatgccggtg ccgccgcctg ggggaacggc acaactggca catctggtgt actcagtgca
      841 gccaacagcc ttgttggtag ttccaccggg gatcaggtca gcagccgaag catttttgct
      901 ctcaccaacg gcaattatgt cgtcaacagc cagctctgga acaacggagc ggccagtcag
      961 gctggggcgg ccacctgggg gaatggcacg actggggtaa ctggggtggt cagcgcgtcc
     1021 aatagtctgg ttgggagcac caccggcgat caaattggct cttcgtttac ctttgccctg
     1081 ccaaatggca attatgttgt ggccagtcca aattgggcca atgggtcagc actccaggct
     1141 ggtgccgcca cctggggcaa tggaacgacc ggtattaccg gaccggtttc tgccgccaat
     1201 agcctggttg gaggtcagat gggggaccag gttggaattt tgaccacagc attgcgaaat
     1261 ggaaactacg tggtatcgag ttcccgctgg aataatggat caaccgtgga tgtaggggca
     1321 gtcacccttg gaaacgggac gactggtacc acgggcctgg tcacagcttc aaacagcctg
     1381 gtcggaagtt cgccaggtga ctctgttgga tcaaatttta tcctggagtt aaccaatgga
     1441 aattttatta cccggacatc ctcctggagg aacggttcag ccaccaacgc cggggccgtt
     1501 acgctggtga atggcgtgac tggactcact ggtcccgtga ctccctccaa cagtctggtt
     1561 gggacaacca tgaatgatgg agttggattt actccggcta ttgccctgac caatggaaat
     1621 tatgtcgttg cgagcccgct ctggaataat ggttcggcaa ctgatgctgg ggcgattacc
     1681 tggggcaatg gcacgactgg ggttaccgga ccggtgacac catccaacag tctggttgga
     1741 acaacaactg atgaccggat tgcatttgga acgctcgtgg ccctgaccaa tggaaatttt
     1801 gtggtgtgta gtcccgattg ggacaacggt ggcaccgttg atgctggcgc cgtgacctgg
     1861 ggaaacggta ccagtggaat cacaggaccc atcacaccgg ccaacagttt ggtcggaagc
     1921 acaaccaatg atcatgtggg aaatctcact cccttgattt tgaccaacgg gaattatgtg
     1981 gtgtgcagcc ggttatggga taacggctca attgttgatg cgggtgccgc cacctgggca
     2041 aatggaacca ctggtatttc tgggccggtg actacgtcaa acagcctgtt tggaacgaca
     2101 gccaatgatc aggttggcaa tgcctttgtt caatctctca ccaatggcaa ttacaccgtg
     2161 gtgagtttgc aatgggacaa cggttctgca gttgatgcgg gtgctgtcac ctgggggaac
     2221 ggtacaactg gcatcacggg cccggtcagt ccagcgaaca gtgtcgttgg gtcaaccacg
     2281 aatgaccaga ttggatttgg ttcaattatt gccctgagca acgggaatta tgtgattcag
     2341 gggccaggat gggacaacgg ttcaacggta aacgctggag ccgccatctg ggtcaatggt
     2401 tcaaccggtc aaaccctcaa tggactcaat accatcagcg ctcaaaacag cattattgga
     2461 tcagttccaa acgcagcagg gtcgtttttt ggcttttttg aagacacggt caacgacagc
     2521 tttttggttt ccttcagcaa tgatccaccc agcggacgaa tcaccgtcgg cgtacccaat
     2581 ccgaatcagt tgacttacaa ccgtggacag gcatccacca tgaccatcac ggcagatttt
     2641 ctcaagcgaa ccctcgatgc gggaaccgat gttgtgctcc aggccaacaa tgacatcacc
     2701 atcaacagcc cgctttcgat tgcagcagcc ggtggagacc tgacccttca ggcaggtcgg
     2761 agcattaccg tcacttccac gctttctgct atcaacagcg acctgaactt gctggccaac
     2821 cagccagtct caaccggcgt cgtgaatgcc caacgggatg cgggcgcggc tgtcattacg
     2881 gcttcagcac caatcaatct tggaaccgga cggtttaccg ctcgcattga cactggttcc
     2941 gggctcacaa atcaaaccaa cggcgccatc acactgagta ccgtcaccgc caatcgaatt
     3001 actatcacca acaatgggcc aacggctgga accgggcgtg tcagagttac ggggacgatc
     3061 accggaggtg cctgcaacgg cgccagcctc tgtactgcca gtggcggcgg gggtcaggtc
     3121 accatcaatc atccaaacag cggtgtggat tttctcatcg gagacgccac aaccaatggc
     3181 acggcaggga cgattgtgac tggaaccgtc acggtggcgg ctccttttac cgtgatgaat
     3241 gttcccggaa cattttcaca ggggaatgtc agcatcagcc ctggtcaaac gttgattcca
     3301 ccagggttgc aagccctttt gatggttgca gataccatca acaaccgggt gcagcaattt
     3361 gacggcacca cctggtcggt catcggagtt ggaacggttg gatcaggtga tggtcaattc
     3421 cgtcaacccg aagcagtcac ctttgacagt acgggtcgaa tttttgtggc cgacaccggg
     3481 aatgatcgca tccagttttc aaccgatagc ggggtgacct gggcgaattt tgccacggtt
     3541 ggttcaggat taaatcaggt tcgtgcgcca caaggactgg cgcttgattc agatggcaac
     3601 ctgtatgttt ccgacacggg caatggccgg gtgatacgct ttaacggagg cattcccggt
     3661 agcggcatcg tgattgccac caatggcgcc ggaggcgggc aggtcggaag cccgcgcgga
     3721 ttaacgattg ataccacctt ccggctcttt gttgcggatg aatctaacag ccggattttg
     3781 cgcattctca atgccaacac cacaaccagc ggaacaagcg ggacaatcat tgcctcgcaa
     3841 ggcacagctt taaacaaagt caagaatcca cagggggtta cggttgattt gacaaccgga
     3901 acactcttta ttgccgatac tggaaactcg cgaattctga gttttcccaa tggaaacgcc
     3961 aataacgcgt cagccattgc gctgacgggt tcgcaattgg gacaggtcaa taaagctgaa
     4021 ggagttaccg tgagtttctt tacggctggg ccatttgccg gaaatgttct gctcgtggtg
     4081 ggtgatacct tcaacaatcg tattcaggga agattcctcc ccaacggcgc ctggtcgttg
     4141 attggcactc caaacaacat cggaagtggc gttggccagt tccgcaaccc gagcaagatt
     4201 cggtaagtgt ttttagggtt cagggttcag ggttcagggt tcaggggttt aaaagacaaa
     4261 aaggacgaaa aggacaaaaa gg
//
LOCUS       DAOQDO010000218         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14102_length_4280_cov_2.997160, whole genome shotgun sequence.
ACCESSION   DAOQDO010000218 DAOQDO010000000
VERSION     DAOQDO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14102_length_4280_cov_2.997160"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2838)
                     /gene="nifJ"
                     /locus_tag="PKZ53_04785"
     CDS             complement(<1..2838)
                     /gene="nifJ"
                     /locus_tag="PKZ53_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNJ39783.1"
                     /translation="MNREKVTLEGNEAAAYIAHKTNEVIAIYPITPSSAMGELSDAWS
                     VAGQTNIFGSIPLVQEMQSEGGAAGAVHGALQTGSLTTTFTSSQGLLLMIPNMFKIAG
                     ELTSTVFHIAARSIATHALSIFGDHSDVMSVRSTGWAMLFGCSPQEVMDFALIAQSST
                     LKARVPFLHVFDGFRTSHEVMKIERIPDEDISAMIDRESVYAHRMRAMSPDRPVIRGT
                     AQNPDVFFQARETINPFYTQTPEIVQDTMDKFAQLIGRQYHLFDYVGDPEAERVIVMM
                     GSGCETAEETVTYLLAQGEKVGLLKVRLYRPFSVEHFLKGLPATVKSIAVLDRTKEPG
                     ASGEPLYQDVITAISEDYINGQVLSPFTRFPKVIGGRYGLSSKEFTPAMVKAVFDELA
                     KDKPKNHFTVGINDDVTHTSLDFDTNFDIEADDVVRALFWGLGADGTVGANKNSIKII
                     GEETPFYAQGYFVYDSKKSGARTVSHLRFGPRPIRSTYLIRRANFIAVHQFGFVEQFD
                     VLENASEGATFLLNSPYGPDEVWDKLPRSLQEQIIRLKLKFYVVDGYSVAKQAGMGNR
                     INTVMQTCFFAISGVLPREEAIEKIKGTIRKTYGKRGEAVVIKNFAAVDQTLENLHQV
                     TVPEQLTSTFERPPVVPASAPDFVRNVEAEIIAGRGDMLPVSAFPIDGTFPVGTTQWE
                     KRCIAQEIPVWETDLCIQCGKCVMVCPHAVIRAKAYDADELTTAPAAFKYTDARFKEL
                     PGKKYSLQVSADDCTGCRLCVEVCPVKDKTQTGRRAINMAPYLPLKEVERENWEFFLK
                     LPDIPVHESLKVDSVKNVQLIQPLFEFSGACAGCGETPYLKLLSQLFGDRALIANATG
                     CSSIYGGNLPTTPWATNKDGRGPTWSNSLFEDNAEFGLGMRLTIDKQTEYARELVHRL
                     RAIIGDNLADGLLNSPQDTEGEIGAQRERVAI"
     gene            complement(3549..4103)
                     /locus_tag="PKZ53_04790"
     CDS             complement(3549..4103)
                     /locus_tag="PKZ53_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39784.1"
                     /translation="MKKSLLILLITLGCWPGISGSNSIFAQDSDFNPNRIAVNQLISA
                     SWPVQIKTAVITQLGKRQLLTCTVTNPGTQTVQNIELLLSVYFPDGSPHGVSLWQQPV
                     TLAPGQSLDFTHQIGTIEDPDKVTDSDDQIGLKIDLQDKVVLSITNVKTDSGGWKTDQ
                     RQSLRSLNRFAMGEFIQPLEPEKY"
BASE COUNT         1007 a         1025 c         1178 g         1070 t
ORIGIN      
        1 gatggcgact cgttcgcgtt gggcgccgat ttcaccttcg gtgtcctggg ggctgttgag
       61 cagaccatcg gccaggttgt cgccgatgat ggcgcgcagc cggtgaacca gctcgcgggc
      121 atattcggtt tgtttatcaa tcgtgagccg catgccgagc ccaaattccg cgttgtcttc
      181 aaacagggaa ttgctccagg ttgggccacg tccatctttg ttggttgccc agggggtggt
      241 tggcaggttg ccgccataaa tactcgaaca ccccgtggcg ttggcgatca acgcccggtc
      301 gccaaagagt tgcgacagga gtttcaaata cggggtttca ccgcaaccgg cgcaggcacc
      361 agaaaattca aagagaggtt ggatgagttg gacgtttttc acggaatcaa ccttgagtga
      421 ctcgtggact ggaatgtcgg gtaacttgag gaaaaattcc cagttttccc gttcaacttc
      481 tttgagtggc aaatacggtg ccatattgat ggcgcggcgt ccggtttggg tcttgtcctt
      541 aaccgggcag acctcgacgc acagccggca acccgtgcaa tcatcggctg aaacctgaag
      601 cgaatatttt tttcctggca gttctttaaa ccgggcatcg gtgtatttaa aggctgctgg
      661 tgcggtggtg agttcgtcag cgtcataggc tttggcgcga attaccgcgt gtgggcagac
      721 cattacgcac ttgccgcatt gaatacagag atcagtttcc cagaccggga tttcctgagc
      781 gatacagcgt ttttcccact gagtggtgcc gacgggaaac gtgccgtcaa tcgggaaggc
      841 actcactggc agcatgtcgc cgcgaccagc gataatttcg gcttcgacgt tgcgcacaaa
      901 atccggggca ctggcgggca cgactggtgg tcgttcaaag gtactggtca gttgttcggg
      961 gactgtcacc tggtgcaggt tttcaagcgt ctggtcaacg gcggcgaaat ttttgatcac
     1021 cacggcttca ccgcgtttgc cataggtttt gcggatcgtg cctttgattt tttcaatcgc
     1081 ttcttcgcgg ggcagcacac cgctgatggc aaagaagcag gtctgcatca cggtgttgat
     1141 gcgattgccc atgcccgcct gtttggccac cgaatagccg tcaacgacat aaaacttgag
     1201 cttgaggcgg atgatttgtt cctggagcga gcgcggcaat ttgtcccaga cctcatctgg
     1261 cccatatgga ctgttgagca ggaaggtcgc gccttcgctg gcattttcga gcacgtcaaa
     1321 ttgttcgaca aagccaaact gatggacggc gataaaattg gcccggcgga tcagataggt
     1381 actgcggatc gggcgggggc caaagcgcag gtgcgaaacg gttcgcgcgc cagatttctt
     1441 cgagtcatag acgaaatagc cttgagcgta aaacggagtc tcttcgccaa taatcttgat
     1501 tgaatttttg ttggcgccaa ccgtgccgtc agccccgagt ccccagaaga gtgcccggac
     1561 gacatcatcg gcttcgatgt caaagttggt gtcgaaatca aggctcgtat gcgtcacgtc
     1621 atcattgatg ccgaccgtga aatggttttt tggcttgtct ttggcgagtt cgtcaaacac
     1681 ggccttgacc atcgccgggg tgaattcttt ggatgacagg ccatagcgac caccgatgac
     1741 tttcgggaat ctggtaaacg gcgagaggac ctggccgttg atgtaatctt cgctgatggc
     1801 ggtgatgacg tcttgataga gtggttcacc tgaggcaccc ggttctttgg tgcggtccag
     1861 caccgcaatg gatttgacgg tggctggcag ccctttcagg aaatgttcaa ctgaaaatgg
     1921 ccgatagagt ctaactttca agaggccgac tttctcgcct tgtgccagca ggtaagtgac
     1981 ggtttcttcg gcggtttcac agcctgaacc catcatcacg atgacgcgtt cggcttcggg
     2041 gtcaccgaca taatcaaaca ggtgatactg ccgcccaatc aactgtgcaa acttgtccat
     2101 cgtgtcctga acgatttcag gtgtttgggt ataaaacgga ttgatcgttt cacgggcctg
     2161 gaaaaagaca tctgggtttt gcgcggtacc gcgaatgacc ggacggtcag gcgacattgc
     2221 gcgcatgcga tgggcataaa cgctttcacg gtcaatcatc gcggagatgt cttcatctgg
     2281 aatccgctcg attttcatca cttcgtgcga ggttcgaaac ccatcgaaga cgtgcaggaa
     2341 gggcacccgg gctttgagtg tggaactttg cgcaatcagc gcaaaatcca tcacttcctg
     2401 cggtgagcag ccaaaaagca tcgcccagcc ggttgaccgg accgacatca catcactgtg
     2461 gtcaccaaaa atggaaagcg cgtgagtggc gattgagcgg gcggcgatat gaaacacggt
     2521 gctggttaat tcacccgcaa tcttaaacat gttgggaatc atcaagagca gtccctggct
     2581 agaagtgaac gtcgtggtca gggagccggt ttggagcgca ccgtggacgg cgcctgcggc
     2641 accgccttca gactgcattt cttgcacgag tggaatactg ccgaaaatat tggtctggcc
     2701 agcaaccgac caggcatcag acaattcccc catcgccgaa gacggggtaa tcggataaat
     2761 ggcaatcact tcattggttt tgtgcgcgat ataagcggcg gcttcatttc cttccagggt
     2821 aactttttcg cgattcattg ctcgtcctac ctttcgctgg ctgtcgtttt cgtcagtggt
     2881 ggctggccaa gaaccgatgc cgttcacttc ctgatgaaat ccaatagcag agggttattg
     2941 aataaaagct ttgttttcaa ctatttgtcg ttatggtttg actgagcgca agtcttctga
     3001 ccagttcgac aaacaggaac cgtgagcaac catcagaatt tctttcacag gcattgggta
     3061 gttgctgtgc tggggaacga tttggggttg tacctcactt gatccagttg aggagcaaat
     3121 cccgtgcctt gtccaaaaag ggggattttc cgcaaaggca gaatgaagaa ttgagaatga
     3181 agaatgaaga attgagaatg acccaaattt tgcccaaaat agcaccgtct tgctcaaaga
     3241 tcagtcccga ccagatcgag atacccctcg gaaacaggaa gtgacccggt cattgagttg
     3301 aatcgaagtt cacccaaatc gagccttcgc ggtgacaaaa ctgactccca cgctatcctt
     3361 tctgtctttt atgtccttga tgtcctttgg tttggtctca atcaactgct actgacacca
     3421 acactgaaga tgagatcact atgttgaggg caagacacaa aaacgccgtg ccagcctcaa
     3481 ctcaattgaa ccgggcagca cggcgctgga tgggaaattt tcgattcttt cgttgtaaga
     3541 agcgaatttt aatatttttc gggttcgaga ggttgaataa actcacccat cgcaaagcga
     3601 tttaatgatc ggagcgattg gcgctggtca gttttccagc cacctgagtc agttttcaca
     3661 ttggtgatgg acaaaaccac cttatcctgg agatcaattt taagtccgat ttgatcatca
     3721 gaatcagtca ccttgtctgg gtcttcgatg gtgccgattt ggtgcgtgaa atcaagtgat
     3781 tgcccaggtg ccagtgtaac cggttgttgc cagagtgaaa cgccatgggg tgacccatca
     3841 ggaaaataga ccgaaagcag gagttcaata ttctgaactg tctgagtgcc tgggttggtt
     3901 acggtgcagg tcaataattg tcgtttcccc aactgcgtta tcacggcggt ctttatttga
     3961 actggccatg aagctgaaat cagttggttg acggcaattc gatttggatt gaaatctgag
     4021 tcctgggcaa aaatggagtt cgagcctgaa attccaggcc agcagccaag agtgatcaac
     4081 aaaataagga gtgatttttt catatatctc caacgcgtga tttttgaaag gtgttgaatc
     4141 aaaggtgaga ttcgtcgtgg gtttaaccct gaagaaggac gctggttcac ctttttgaaa
     4201 gccagaatga aatctccaaa aaaacaccca ggccagttga actggcctgg gtggcggatt
     4261 gcagtgatta agaaggatct
//
LOCUS       DAOQDO010000219         4276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14125_length_4276_cov_4.506752, whole genome shotgun sequence.
ACCESSION   DAOQDO010000219 DAOQDO010000000
VERSION     DAOQDO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14125_length_4276_cov_4.506752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..4227
                     /locus_tag="PKZ53_04795"
     CDS             16..4227
                     /locus_tag="PKZ53_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNJ39785.1"
                     /translation="MIHVNAGGELFQSLVELKVKEAPQGVTATFDRKWITAGATATLK
                     VAVAKGKVLPGSYKLTVEGEAAVGGGMVRREAVLTLQVKPLLRTALTGRVLSTEMEPI
                     PGATVSLDGRSATTDGAGMFVLEGIEAGQNRPVMVDGRTATAPNRTYPVIVEPATVIF
                     NRANTVPYTFFLPAIDTQYEVPVAPGQETVVTTPRVPGLKLTIPAEANLRNRDGSPVT
                     RVSLTPVAIDRTPAPLPAGINLTMVFTAQPGGADSDVAMPVSFPNLTAETPGTSFSLW
                     NFNHDSAQWEVYGTGHVSPDGRTIEPDINPSTGKPFGLPYFSWGGPGTNLANDSSGPG
                     PGGPFPGGPGDPANCPSCSSDNPVNFATGFKVETETDFGWGGARGGLSLTRTHLNTQA
                     VGRFGLGWSDNWTMTVSDSRDGFAASISVSGQNRPWLFSLARTEEIEGAVEPVKVYQN
                     TQQAGMLGDELKYYPERIQNGTNEPAFWEYRSKGGTKLRFNQTGRWETMSDRNGNTTV
                     LSYDERGRLVKVTDPVGRWMEFTYRPAGRTTGTENQVIAVTDVMQRRWEYQYGPDQGD
                     ALNPDFNAFLLLSVTDPLGHTMRYEYIALARVFGAVRDGGRLKQVIDKRGNIVKDIEY
                     ESGGTGRVISQKFADGGVERYSYTVAGGQVTGVTITDPMGRVTSRRFNTAGYVIETTD
                     ELGQVRRVEREIGTNLPIELIGPNGVSESRRLYNKNGETIEIQDILGHKTTFDYEDKY
                     NNIVSMTDTLNKKILREYDSNGNWIKLIDSYSQQKKFFYDELGQLIGIEDENENKTQM
                     DYDSFGNLIRLRDSLGYQIEYTYNVLGWITLIKDPIGRTQQFEYDLVGNQLSFIDPTV
                     GKNKYEYDENGNKKRIIDDLNRVLDMEYDSKNRLIKRIDPLGRQTVWSYNYNDELISL
                     ILPSNRKIQYQHDQRGFLISTLDSGGTEVKYIIDSKSNLIQVINQRHFAVTYFYDRLN
                     RLIKVVDQQGKSIEMKYDVEGKLIERKDKLGIISRFKYDDLERVIEVAYPDDTISYEY
                     DKVGRRTKVTDSQGGEIRWKYDSNDRIVEEITPNGVIQYNYNEAGQRVEMKISGHPST
                     FYEYDSLGRLESLSQGQDRFSYAYDSISRIVKLTSTKGMESTFEYDAVNNTKKIRHST
                     GKMGLIEQLDYTYSLDDEVLSISSSNEQQIELPLDKAIGIAAPANQINQFDKGAYEYD
                     DAGRPTVKVETAGITHYEWDSRHRLVKVNLPNQKEIQYSYDALGRMTTRACDGQIKKF
                     LYDQKDVIVDTESNGNKVLYLNDLRVDVKLKQSEGSKNYYYFRDKNGSTTVLTDENGE
                     VIEKISYEPFGNSKRSKVRFGFTGREGDQDTGLYFFRARWYNPEQGRFLTEDPLDQIV
                     KLNLYLYASNNPNRFTDPFGLQNSL"
BASE COUNT         1313 a          831 c         1126 g         1006 t
ORIGIN      
        1 agcgaaaccg cgtttgtgat ccacgtcaat gcgggcggcg agttgttcca gtcgctggtg
       61 gaactcaaag tgaaggaagc tccgcaaggg gtaaccgcga cctttgaccg gaaatggatt
      121 accgccggag caacggcaac gctaaaagtc gccgtggcca aaggcaaagt gttgccggga
      181 agctacaaac tgacggtcga gggtgaagcc gccgttggag gtgggatggt ccgccgggaa
      241 gccgtgctca cgttgcaggt gaaaccgcta ctccgaaccg cgttgaccgg gcgcgtcctc
      301 tcaaccgaaa tggagccgat tccaggggca acggtctcgc tggatggtcg gagcgcgacc
      361 acggacggcg ccggaatgtt tgtgttggaa gggattgaag cgggccagaa tcgtccggtg
      421 atggtggatg gccggaccgc gacggcgccg aatcggacct acccggtgat tgtcgaaccg
      481 gcgacggtga ttttcaatcg ggcgaataca gtgccgtaca cgtttttcct gcccgcgatt
      541 gacacccagt atgaagttcc ggtcgctccg ggacaggaaa ccgtggtcac gacaccgcgt
      601 gtcccagggt tgaagctgac gattccagcc gaagcgaatt tgagaaatcg ggatggttcg
      661 ccagtgacgc gggtatcgtt gacaccggtg gcgattgacc ggactccagc gccgttgccc
      721 gctggaatca acctgacgat ggtgttcacc gcccaacccg gaggtgccga ttcggatgtg
      781 gccatgccgg tgagtttccc gaacctgact gcggaaacgc caggaacatc ctttagtttg
      841 tggaacttca atcacgattc ggcgcagtgg gaagtgtatg gaacgggtca tgtaagcccc
      901 gatggtcgca cgattgaacc ggacatcaat ccgagcaccg ggaaaccatt tggattgcct
      961 tacttttcat ggggtggacc gggtaccaat cttgccaatg actcaagcgg tcccggccca
     1021 ggtggaccat ttcccggcgg ccctggagac ccggcgaact gtccgtcgtg cagcagcgac
     1081 aatccggtca atttcgcgac cgggttcaaa gtcgaaacgg aaaccgattt tggatggggt
     1141 ggggcgcgtg gcggcttgag tctgacccgg acccatttga acacccaggc ggtcgggcgg
     1201 ttcgggcttg gctggagcga caactggacc atgacagtca gtgattcccg ggatggtttc
     1261 gcggcctcga tctcagtttc aggccaaaac cggccatggc tattcagcct ggcgcgaacc
     1321 gaggaaatcg aaggcgccgt ggaaccggtc aaagtctacc aaaacacaca acaagccggg
     1381 atgttgggtg acgaattgaa gtattatcca gaacgaatcc aaaacgggac gaatgaaccg
     1441 gccttttggg aataccggag caaaggcgga acgaaactga gattcaacca gaccgggagg
     1501 tgggaaacga tgagcgaccg gaatggaaat acaacggtgt tgagctatga cgagcgggga
     1561 cgactcgtga aagtcacgga cccggtaggc cgctggatgg agttcaccta ccgaccggca
     1621 gggagaacga ccggcacgga aaaccaggtg atcgcggtga cggatgtgat gcagcggcgg
     1681 tgggaatatc aatatgggcc ggatcaggga gatgcactca acccagattt caatgccttc
     1741 ctgttactgt cggtgacgga tccgttgggg cacacgatgc gctatgaata catcgccctt
     1801 gcccgagtct ttggcgccgt tcgtgacgga ggccggttga aacaagtgat tgacaagcgt
     1861 ggaaacatcg tcaaagacat cgagtacgaa tcaggcggta cgggtcgggt gatcagccag
     1921 aagttcgcgg atggcggagt tgaacggtat agctacacgg tggcgggagg ccaggtgacg
     1981 ggagtgacga tcacggatcc catgggccgg gtgacgagtc ggagattcaa caccgccggc
     2041 tatgtgattg aaaccacgga tgaattgggg caggtccgac gagtagaacg cgagattggg
     2101 acgaatttgc cgatagaact aattggccct aatggagtaa gcgaaagtcg gcgattatac
     2161 aacaaaaatg gagaaacgat tgaaattcag gatatacttg gtcacaaaac aacgtttgat
     2221 tatgaagata aatataataa tatagttagt atgacagata ctctaaataa gaaaattttg
     2281 agggaatatg attcgaatgg gaattggata aaactaattg actcgtatag tcagcaaaag
     2341 aaattcttct acgatgaact tggtcaatta attggcattg aagacgagaa tgaaaataaa
     2401 acacaaatgg attatgattc atttggaaat ctcataagac ttagagatag tcttggatac
     2461 caaatagaat acacatacaa tgtattaggt tggattacat taatcaaaga ccccattgga
     2521 agaactcagc agtttgagta tgatctggtc ggtaatcaat taagttttat tgatcctaca
     2581 gttgggaaga ataaatatga atatgatgag aatggtaaca aaaaaagaat tattgatgat
     2641 cttaatcgag ttctagatat ggaatatgat tcaaaaaacc gactgattaa aaggattgat
     2701 cctttgggaa gacaaacagt ttggagttat aactacaatg atgaacttat atcgcttata
     2761 ttgccctcaa atagaaagat tcagtatcaa cacgaccaac gagggttttt gataagtaca
     2821 ttggattcag gtgggactga agtgaaatat ataattgatt cgaaatcaaa tcttattcaa
     2881 gtaataaatc aacgtcattt tgccgtaact tatttttatg acaggttaaa tcgattgatc
     2941 aaagtggtag accagcaagg gaaaagtata gaaatgaaat atgacgttga aggcaaactt
     3001 atcgaaagaa aagataagtt aggaataatc agtcggttta aatatgatga cttagaaagg
     3061 gtgattgaag tggcctaccc tgacgataca atctcatatg aatatgataa agttggaaga
     3121 agaactaaag taactgatag ccaaggagga gagattagat ggaagtatga ttcaaatgat
     3181 agaatcgtag aagaaatcac acctaatggt gttattcagt ataactataa tgaagctggt
     3241 cagagagttg agatgaaaat tagcgggcac cctagcacct tttatgaata tgattctttg
     3301 ggtagattag aaagcctttc acagggtcaa gatagatttt cgtatgcata tgattcaatt
     3361 tctagaatag taaaacttac ttctaccaaa ggaatggaat ctacctttga atatgatgct
     3421 gtcaataata ctaaaaagat tcgacattca actggaaaaa tgggattaat tgaacaatta
     3481 gattatactt attctttgga cgatgaagtt ttatcaattt catcatcaaa tgaacagcaa
     3541 atagagcttc cactggataa agctatcgga atagctgcac cagcaaatca aattaaccag
     3601 tttgataaag gggcttacga atatgatgac gctggtcgtc ccactgttaa agtagaaacc
     3661 gctggaatta ctcattatga gtgggattca agacatcgat tagtcaaggt aaatctgcct
     3721 aatcaaaagg agattcaata ttcttatgat gccttgggaa gaatgacaac tcgtgcgtgc
     3781 gatggtcaaa taaaaaaatt cctttatgat caaaaagatg taattgtaga cactgaaagt
     3841 aatggaaaca aagttttata cttaaatgat ctaagagtag atgtaaagct aaaacaatca
     3901 gaagggagta agaattatta ttattttaga gacaaaaatg gaagcactac tgtattaacc
     3961 gatgaaaatg gtgaggtgat tgaaaaaata tcttacgagc cgtttggtaa ctcaaaaaga
     4021 tctaaagttc gatttggctt tactgggaga gaaggggatc aagacactgg cttatatttt
     4081 ttcagagccc gttggtataa tcccgaacaa ggtcgattcc taacagaaga ccctcttgac
     4141 cagatagtta aattaaattt gtatttgtat gcttctaata acccaaatcg atttacagat
     4201 ccatttggtc ttcagaatag cttataagta ccttttcata aatacactga tataataagc
     4261 aagctgagat caggta
//
LOCUS       DAOQDO010000220         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14184_length_4267_cov_2.852327, whole genome shotgun sequence.
ACCESSION   DAOQDO010000220 DAOQDO010000000
VERSION     DAOQDO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14184_length_4267_cov_2.852327"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..749
                     /locus_tag="PKZ53_04800"
     CDS             108..749
                     /locus_tag="PKZ53_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNJ39786.1"
                     /translation="MAQTKNTAASVDEVKQKRHSEDPEAQELKRRVGETIERLRRSRG
                     LSLERLATLSGVSRTMLSQIETGESAPTISLLWKVSRGLAVRFADLLGDEASDDVRFL
                     PAKSSKVLRSGDGSFESRALFPFDTQRRAEFYELRLRSGHTERAEAHASGTYENLVVH
                     SGKLRLVVGDQPPLELNAGDAVYFRADVPHVYENSWKQDTVMYLVMTYSEVEK"
     gene            1098..2012
                     /locus_tag="PKZ53_04805"
     CDS             1098..2012
                     /locus_tag="PKZ53_04805"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNJ39787.1"
                     /translation="MTHDLIDQIGNTPLLRLRAFEKEVPAGVELYAKAEYLNPSGSVK
                     DRGILSIILDGEERGQLTHDKIILDATSGNAGISYALIGAARGYQVHLCISKNASPER
                     KRILRALGAHLIETDPLQGSDGAQIEARRLVDANPGIYFYPDQYNNPANWLAHYRTTA
                     PELWHQTEERLTHFITGIGTSGTFTGTTRKLRELNPDIQCISVQPDLPMHGLEGMKHM
                     ATALVPGIYDPHLADDNVEVSTEDAQATTRRLAREAGIFVGLSAGANVFAAIRLAQHL
                     PFGSVVATILCDSGTRHLSDPFWDEVTN"
     gene            2052..2498
                     /locus_tag="PKZ53_04810"
     CDS             2052..2498
                     /locus_tag="PKZ53_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNJ39788.1"
                     /translation="MIELSIEHIESIQHHGEADYPYECCGLLIGHFSSDGKKQVVEVY
                     PISNAREEAAKRNRFLILPQELMRGERYARSKKLDVVGFYHSHPDHPAIPSGFDQDHA
                     WPTYSYIIVAVANGQARDVLSWELEADRSRFNPETINMCAGVLVAG"
     gene            2603..4078
                     /gene="moeB"
                     /locus_tag="PKZ53_04815"
     CDS             2603..4078
                     /gene="moeB"
                     /locus_tag="PKZ53_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNJ39789.1"
                     /translation="MSVTIFIPTALRQFAGGNTEFSCDALTVADALTFLTTTHADLRK
                     HLYSDAAKLRSFVNVYVNDTDIRHSSGLETPIKDGDTVMIVPAIAGGVEVAEHLAEAT
                     HHHPVELSSAELARYSRHLILPEVGIDGQKKLKAARVLMIGTGGLGAPLGLYLAAAGV
                     GRLGIVDFDVVDASNLQRQVIHGTKDVGRPKIASARDRLLDINPHIEVIPYETRLTSQ
                     NALELFRDYDVIVDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFEGQATVFWAEKGAC
                     YRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGSIQAAEVIKIILGAEDTLINRLIHFDA
                     WRMRFKELKLRKDPNCPICGPNRTINALIDYEEFCGLKPPPAAPGSSPHAELAEITAV
                     ELKQRIDAGENLQIIDVREPYEYEIARLPYTKLIPLGQVVGRQSEIDPTQTTVVHCKM
                     GGRSAKAIAQLKEAGFTGRLINLKGGITAWSTDVDPSVPKY"
BASE COUNT         1034 a         1067 c         1097 g         1069 t
ORIGIN      
        1 aaactgttcg tcccgaaaac gagatggctc gaaaaatatc tccgtaacct gaccggtcgg
       61 tagcttttca agggacacag accggtcttt accgcttcat tcaatatatg gctcaaacaa
      121 aaaacaccgc tgcctctgtg gatgaggtaa aacaaaaacg ccactccgaa gaccctgagg
      181 cgcaagaact caagcgccgg gttggtgaaa ccatcgagcg actacgtcgc agccgaggtc
      241 tgagcctgga gcggctggcg accctttcag gcgtcagccg aaccatgctc agtcaaattg
      301 aaaccggtga gagcgcccca accatttccc tcctctggaa ggtttctcgg gggttagcgg
      361 tacgatttgc tgatttgctg ggggatgaag ccagtgatga tgtccggttt cttccagcca
      421 aatcttcaaa agtgctgcgc tctggcgatg ggagctttga gagccgggcg ctcttcccgt
      481 ttgatactca gcgtcgggcc gaattttatg aactccgact gcgaagcggc cacactgaac
      541 gcgccgaagc ccacgcctcg ggaacctatg aaaacctggt tgtgcattcc ggtaagctca
      601 ggctggtggt gggtgaccag ccccctctgg aattaaatgc cggggatgcg gtttattttc
      661 gggccgatgt tccccacgtg tatgagaatt cctggaaaca ggataccgtc atgtatctgg
      721 tcatgaccta tagcgaggtt gagaaataga aacatttttc cagtcccaat gcgtagggat
      781 gtgcactcat tcctgtactt ggcaaactga ggttgcttgg aaaatgttct gcaccagcaa
      841 gttccagtca ttactgctgt cacctgatct caggtgtcgc cagttttctg ttgatcaatt
      901 caacatccca ctcccccctg acatagcttg aatcaagtga cagatctgcg gtccttgatt
      961 gacttaaggt gaaagtatag cgtacaagat gacgttatag tgatgatttc atgattcttg
     1021 agctggtaag ggagtttgca atgggagcta tcttcatgaa acggtccccg acaataaacc
     1081 aaatcccagg gttaaaaatg acccatgacc tgattgacca gattggaaat actccgttgc
     1141 tcaggctccg ggcatttgaa aaagaggtgc cagccggcgt cgaactgtat gccaaggcgg
     1201 aatatttaaa tccaagtggt tcggtcaagg atcgtggcat tttatcaatc atcctggatg
     1261 gtgaagaacg tgggcagctc actcacgata aaattatttt ggatgcgaca agtggaaatg
     1321 ccggaatttc ctacgcgctg atcggtgccg ctcgcggata tcaggttcat ctgtgtatct
     1381 cgaaaaatgc cagccccgag cgaaagcgaa tcttgcgggc actcggcgcc catctgattg
     1441 aaactgaccc gcttcagggt tcagacgggg cacagatcga agcccgccgg ctggttgatg
     1501 ccaatcctgg gatttatttc tatcctgatc agtacaacaa cccggctaac tggctggccc
     1561 attaccgtac aacggcccct gaactgtggc accagaccga agagcggcta acacacttca
     1621 tcactggtat tggcacctct ggtactttta ccggcaccac ccgcaagctc cgtgaattaa
     1681 accctgacat tcaatgtatt tcggtccagc cagacctgcc catgcatgga ttggaaggga
     1741 tgaagcatat ggccacagcg ctggtgcctg gcatttatga tccgcatctg gctgatgaca
     1801 atgttgaagt atcaactgag gatgcccagg ctaccactcg ccggctggct cgcgaggccg
     1861 ggatttttgt tggcttgagt gctggcgcca atgtctttgc cgcgatcagg ttagcccaac
     1921 accttccctt tggatccgtt gtggccacga ttttgtgtga ttcaggaacc agacatttga
     1981 gcgatccttt ttgggatgaa gtcaccaact gagtgaagtc ttcaatccgg ttaaattttt
     2041 tggagggaaa tgtgattgaa ctttcaattg aacatattga atccattcag catcacggag
     2101 aagccgatta tccatatgaa tgctgcggcc tcctcattgg ccatttttcc tctgatggga
     2161 agaagcaggt cgtcgaagtg tatccgattt caaatgcccg cgaagaagcc gctaaacgca
     2221 accggttttt gatcctgccc caagaattga tgcgtggtga gcgatatgcc cgatcaaaaa
     2281 agctggatgt ggttggtttt tatcactcgc atccagatca cccggctatt ccttcaggct
     2341 ttgaccagga ccatgcctgg ccaacctatt cgtacattat tgtcgctgtg gccaatggac
     2401 aggccagaga tgtgctgtcg tgggagttag aagctgatcg aagccgattt aatccggaga
     2461 ccatcaatat gtgtgccggt gtattggtgg cagggtagct taaagtgaaa tggtttcttt
     2521 tgtaacatac tgaatcaaaa gaacaaattt ttaaaaagaa caattcttca gtgatccttc
     2581 actaattcca ggagcttgat ctatgtcagt gaccattttt atcccgaccg cactcaggca
     2641 gtttgctggt ggaaacaccg agttctcatg cgacgccttg acggtggcgg atgccttaac
     2701 ttttctgacg accacccacg ctgatcttcg caaacacctg tacagtgacg ccgccaaact
     2761 gcggagcttt gtcaatgtct acgtcaatga caccgatatt cgtcattcat cagggttgga
     2821 aacgccaatc aaggatggtg acacggtcat gattgttcct gcgattgcgg gcggtgtcga
     2881 ggttgcggag catcttgctg aggccacgca ccaccacccg gttgagcttt ccagtgctga
     2941 actggcccgg tatagccggc acttgattct tcctgaagtc ggaattgacg gtcaaaaaaa
     3001 actgaaagcc gcccgtgtcc tgatgattgg aaccggtggg ctgggtgctc cactgggcct
     3061 gtatctggca gccgccggcg tgggacgcct gggcattgtt gattttgacg tagttgatgc
     3121 ctcaaatctg caacggcagg taatccatgg cacaaaggat gttggtcggc caaagattgc
     3181 ttcggcccgg gaccgattgc tcgatatcaa cccacatatt gaagtgattc cctatgaaac
     3241 ccgcctgacc agccagaatg ctctggaatt atttcgtgac tatgacgtga ttgtggatgg
     3301 aactgacaat ttccctaccc gctatctggt caacgatgcc tgtgtactgc tgggcaagcc
     3361 caatgtctat ggctcaatct tccggtttga aggacaggca acggtgttct gggctgaaaa
     3421 aggcgcctgc taccgatgtc tctaccctga acctcctcca ccaggattgg tgccttcctg
     3481 tgctgaaggc ggcgttttag gagtgttacc aggaattgtt ggctcgattc aggcggctga
     3541 agtcatcaaa atcattcttg gtgctgaaga taccctgatc aatcggctga ttcattttga
     3601 cgcgtggcgg atgcgcttta aggaattaaa attgcgcaaa gaccccaatt gcccaatttg
     3661 tggtccaaac cgcacgatca atgccttgat tgactatgag gaattctgcg gattgaagcc
     3721 acctccggca gctcccggca gctcaccaca cgctgaactg gctgaaatca cagccgtgga
     3781 attaaaacag cgaattgatg ccggggaaaa cctgcaaatt attgatgtgc gtgagccata
     3841 tgagtatgaa atcgcacggt tgccctatac aaaactgatc ccactcgggc aggttgttgg
     3901 tcgtcaatct gaaattgacc cgacacaaac aaccgtggtg cattgcaaaa tgggtggtcg
     3961 gagcgcaaaa gcgattgccc aactcaaaga agctggcttt accggtcgtt tgatcaacct
     4021 caaaggcggc attaccgcct ggtcaacgga tgttgatccg agtgttccaa agtattgaaa
     4081 aactgggctg aagaagtcgg gctgaagaca cagcgggctg aagatatcgg gcttggggta
     4141 gttgaaagga tgaagttctt tagcccaatg ttttcagccc gacttcttca gcccgctgtt
     4201 ttcagcccaa tttcttcagc ccgacttctt catcccgctg ttttcagccc gacttcttca
     4261 tcccgct
//
LOCUS       DAOQDO010000221         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14190_length_4265_cov_2.302375, whole genome shotgun sequence.
ACCESSION   DAOQDO010000221 DAOQDO010000000
VERSION     DAOQDO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14190_length_4265_cov_2.302375"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="PKZ53_04820"
     CDS             <1..2096
                     /locus_tag="PKZ53_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39790.1"
                     /translation="TKTLDNLFSNKILAKESELRILVTDVLTAVAEHSHNRVLEQAYL
                     QAQGLLVGTTTLTGALSDCLMKEISSLKIQILTYEERLAQFTFDFVGSRERNEAGQEV
                     WTGGKYREAWNNLTTGVFAHTSFVDGPELERLYGELARYPAEQFLGIKTEIELKLGND
                     QMPLSIFHCLLERPEEVKRLVFELARRRFRAVQETSIAEKLETLPQSEVDRRIEEAFQ
                     RSHPLIRFDQNALFANDATTGTRLTHHNLKFHNIRKMATCRVQDDPLLVVSGVGESES
                     KLAKAYRSKVTGTEPTQNLPDRYRIVFAQERGVFPLYCLSDLPEIRKAYVYEMRQEKA
                     RPRETNRKIVFPDLLPPDPRESGMPRRVKKALTLGKVFSLIQSGTDVQTEEPQVLYTY
                     CDEQFTKHVIPLGGTFEEALQKLVKRQLDKEIHAQRPDEETHLEHLEGTLKREGQKPT
                     TVTQKAKNWELMQTYLINLAHEIPGGEQNPVFQEERDFINEFRIEYGWKPPANWAPPA
                     GDAVATSGTTPTEGIPSPTSGSDDEAAYRRRIRFMLGKKQGGLPSAEETERWIRQGVE
                     DFHLSPAQAQAILSNEQQPKTSPSTDKLKLERYREECRITMEGGFLSSEGREILDDVR
                     RRLGLTNDEAIQIEKEIMPEEKYRQTCREVIVNRTIPDDGQEILEDSRKRFQLNFERA
                     QAILQAVLEEPLFRN"
     gene            2111..3748
                     /locus_tag="PKZ53_04825"
     CDS             2111..3748
                     /locus_tag="PKZ53_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ39791.1"
                     /translation="MRQFLVLSIICLILPWGSWWNVRTLDQERGQQVRPKMVPRLALV
                     IGNGAYPSASLKNPINDADDMAQTLEKLGFEVLKGTNLPQREFLRLIATFGARLRGGG
                     VGLFYFAGHGIQVGGENYMLPVDAQLETEADVDTYGINVNKVLKQIGSAGNQMNIVIL
                     DACRNNPYVNRNWRETTQGLAEVKAPSGTIIGYATAPGATANDGNGRNGLYTQELLAA
                     LPQMGIPIEQTFKMVLRQVREKSGGKQIPWISSALEGDFCFVENGPGNAGKTDPVTVE
                     LEFWDRIKNSSSRKDFDLYLAEYPAGLHAPLARLRLTELEPGGLPNSLKQITNRLGME
                     FVWIPSGTFRMGDDNHSPDEKPVHPVTLSHPFYLGKYEITQAQWRAVMGYNPSFFKGD
                     NLPVEQVSWLEVQQFIQKLNDQGDGKYRLPTEAEWEYACQAGNQGNEGKVLDQAGWFA
                     GNTGNTTHPVGQKAPNSWGLYDMQGNVWEWCQDVYGKYPTHPVTDPIGNEGKSLRVNR
                     GGGLFSEIQYCQPTTRHFGKPDQRSLDLGFRLLKIAR"
     gene            3756..>4265
                     /locus_tag="PKZ53_04830"
     CDS             3756..>4265
                     /locus_tag="PKZ53_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39792.1"
                     /translation="MGPQFIQFVLCLLVIFGWPSVAVLADAPHPIGIQSTANPTQGKT
                     AAEYLQSGVDWFIKGEYDKAIADCTEALRLDSKLDKGYFFRGAAYSMKKNYNQAIADC
                     TEAIHLNPKEAFYYFNRAVIYGNKGEYDQVITDCTEAIRLDPNNADFYSKRGYAHRKK
                     LDFDKVIADC"
BASE COUNT         1188 a         1026 c         1102 g          949 t
ORIGIN      
        1 taaccaaaac tctggataat ctcttttcca acaaaattct ggccaaggaa agtgaattac
       61 ggattctggt tacggatgtg cttaccgctg tggctgaaca cagccataat agagttttgg
      121 aacaagccta tctccaagct caagggctgc tggtcgggac caccaccttg accggcgcgt
      181 taagcgactg cctgatgaag gaaatctcca gcctcaaaat acagattctc acctatgagg
      241 agcggcttgc ccagtttact tttgatttcg tgggttcccg ggaacgaaac gaagcaggcc
      301 aggaagtctg gaccggagga aaataccggg aagcctggaa taatctaacg accggtgttt
      361 ttgcccatac cagctttgtg gacggtccgg aactggagcg gttatatgga gaactggccc
      421 gctacccggc ggaacaattt ttgggaatca aaactgaaat cgaactgaaa ctcggaaacg
      481 accaaatgcc tttgagtatt ttccactgcc tcctggaacg tcccgaggaa gtcaaacggc
      541 tcgtatttga actggcccgc agacggtttc gggcagtcca ggaaacctcg atcgcggaaa
      601 aactggaaac gttgccccaa tctgaagtgg accgccggat tgaggaggcg tttcaacgtt
      661 cacatccact gatccggttt gaccagaatg cgctttttgc aaacgatgcc accactggta
      721 cccgattgac ccaccataac ctaaaattcc acaatattcg caaaatggcg acatgtcggg
      781 ttcaggatga tccgttgctg gtcgtttcag gggtcgggga aagcgaatcc aaattggcca
      841 aagcctatcg cagcaaagtg accggaacgg aacccacgca aaatttaccg gaccgctatc
      901 gcatcgtttt tgcccaggaa cgaggggttt ttcctttgta ttgtttaagt gacctgcctg
      961 agatccgcaa agcctacgtt tatgaaatga ggcaggaaaa agcccgcccg cgggaaacca
     1021 accgaaaaat tgtctttccg gatttgctgc cccctgaccc tcgggaaagc ggaatgcccc
     1081 ggcgagtcaa aaaggcactc acgctcggaa aagtattttc cctcattcag tctggaaccg
     1141 atgttcaaac agaggagcct caagtgctgt atacctattg tgatgagcaa tttacaaagc
     1201 atgtcatccc gcttgggggg accttcgagg aggcgctcca gaagctggtt aaacgccaat
     1261 tggacaagga aattcatgcc cagcgtcccg atgaggaaac ccacctcgaa cacctggaag
     1321 ggaccctgaa aagggaaggc caaaaaccga ccaccgtcac ccaaaaggcc aaaaactggg
     1381 aattgatgca aacctatttg atcaacctgg cacatgaaat tccaggcggg gaacagaatc
     1441 ctgtttttca ggaggaacgg gactttatta acgaatttcg gattgagtat gggtggaaac
     1501 caccagctaa ctgggccccg cctgcaggtg acgccgttgc aacgtccggg accaccccaa
     1561 ctgaaggaat cccgtctccc accagtggaa gtgatgacga agctgcttac cgcagacgga
     1621 ttcgattcat gttggggaaa aaacagggag ggttgccatc cgctgaggaa accgagaggt
     1681 ggatacgaca gggggttgag gatttccatc tgagccctgc tcaggcgcaa gccattttgt
     1741 ctaacgaaca gcaacccaag acctcaccat ccaccgacaa attgaaattg gagcgctacc
     1801 gggaggaatg cagaattacc atggagggcg gttttctttc cagtgagggc cgtgaaatcc
     1861 tggatgatgt tcgccgccgg ttgggtttga ccaatgatga agccattcaa attgaaaagg
     1921 agatcatgcc tgaagaaaaa taccgtcaga cttgccggga agtcatcgtc aaccggacca
     1981 ttccggatga cggtcaggag attctggaag acagccggaa acggttccaa ctcaactttg
     2041 agcgcgctca agccattctc caagccgttt tggaagaacc attgttccga aactgaaaaa
     2101 aggagtagct gtgagacaat tcctggtcct gagcatcatt tgcctgatat tgccctgggg
     2161 gagttggtgg aatgtacgca ccctggacca ggaaagaggc caacaagtcc gccctaaaat
     2221 ggtcccaaga ctggctttgg tgattggaaa cggagcctat ccatccgcat ccctgaaaaa
     2281 ccccatcaat gatgcggatg atatggctca aacactggaa aagttaggtt ttgaagtttt
     2341 gaaggggacc aacctgcccc agcgtgaatt tctccggctg attgcaacct ttggagccag
     2401 attgcggggc ggaggagttg gcttgtttta ctttgccggg catggaatac aggtgggagg
     2461 ggaaaactac atgcttccgg tagatgccca attggagacg gaagcggatg tcgataccta
     2521 tgggataaat gtcaacaaag tcttgaagca gatcgggagc gccggaaatc aaatgaacat
     2581 cgtgatattg gatgcctgcc gcaacaatcc atatgtcaat cgcaactggc gggaaacgac
     2641 tcaagggctg gccgaagtaa aggctcccag tggaaccatc attggatatg cgactgctcc
     2701 gggtgcaact gcaaatgacg gaaatggtcg caatgggttg tatacccagg aactcctcgc
     2761 tgcccttccg caaatgggca ttccaattga acaaaccttt aaaatggttt tacgccaggt
     2821 tcgggaaaaa agcggaggca aacagatccc ctggatatca tcggctctcg aaggggattt
     2881 ttgctttgtg gaaaacggtc cagggaatgc tggaaaaaca gatcctgtaa cagtggaact
     2941 ggaattttgg gaccgtatta aaaacagttc cagtcgaaaa gactttgacc tgtatttagc
     3001 cgagtatccg gctggattgc atgccccgct tgccagattg aggttgactg aattggaacc
     3061 agggggattg cctaattcgc tcaaacaaat cacaaacagg ctcggcatgg agtttgtgtg
     3121 gatcccttcc gggacattcc gaatgggaga tgacaaccat tccccagatg aaaaacctgt
     3181 ccatccggtc accctcagcc atccttttta tcttggaaaa tatgaaatca cccaagccca
     3241 atggcgtgca gtcatgggct ataacccttc gtttttcaag ggtgataacc tcccggtcga
     3301 acaggtctcc tggttggaag tccagcaatt catacaaaaa ctcaatgacc agggggatgg
     3361 gaaataccga cttcctacgg aagcagaatg ggaatatgcc tgtcaggcag ggaatcaggg
     3421 aaatgaaggt aaggttttgg atcaggcagg gtggtttgcc ggcaacaccg gcaacaccac
     3481 tcacccggtt ggacaaaaag cgccaaactc atggggtttg tatgacatgc aggggaatgt
     3541 ctgggagtgg tgccaggatg tctatgggaa atacccaacc caccctgtta ccgacccgat
     3601 cgggaatgag gggaaatcac tgcgagtgaa tcgtggagga ggtctttttt ccgaaatcca
     3661 atattgtcaa ccgactaccc gacactttgg caagcctgac caacggtcgc ttgatcttgg
     3721 attccgtctt ttaaagattg cgaggtgaaa aaggaatggg cccacaattt attcaatttg
     3781 tactttgttt gctagtcatt tttgggtggc cttcagtagc agttttggcc gacgcccctc
     3841 acccaattgg tatccagtca actgcaaacc caacccaagg aaaaaccgca gccgaatatc
     3901 tacagagtgg cgttgattgg tttattaaag gcgagtatga caaggcgatt gccgattgca
     3961 ccgaagccct ccgcctcgat tcaaaactgg ataagggcta cttttttcgt ggtgctgcat
     4021 actccatgaa gaaaaactac aaccaagcaa ttgccgactg tactgaggcc atccacctca
     4081 atccaaaaga ggccttttat tacttcaatc gtgcagtaat ctacggaaat aagggcgagt
     4141 acgatcaggt aattacagat tgtacagaag ctatccggct agaccccaat aatgcagatt
     4201 tttactccaa acgtggttat gcacacagaa aaaaattgga tttcgataaa gtaattgctg
     4261 actgc
//
LOCUS       DAOQDO010000222         4259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14230_length_4259_cov_2.385109, whole genome shotgun sequence.
ACCESSION   DAOQDO010000222 DAOQDO010000000
VERSION     DAOQDO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_4259_cov_2.385109"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..344)
                     /locus_tag="PKZ53_04835"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="PKZ53_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            659..2044
                     /locus_tag="PKZ53_04840"
     CDS             659..2044
                     /locus_tag="PKZ53_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNJ39793.1"
                     /translation="MAKRASNEPWVLERRAFLQLGAAGLTSLLVGWPNPAKAETLSRL
                     ATGVRAVTPRNSARNCILVMLRGGPSHVDMFDLKKGRWTPNELGVERLSNGIDWPTGI
                     LPRLKTQLNKCSIIRSLQHSEVVHERAEYFVETGRRLNPGLRNEVPHLGAVISLESEA
                     QRTAQDVFPSFMLFNYGFSYSNNGFLSATHAPFNVFYPAYGIPNLDPPDGPESFNRRR
                     AALQFVDGIGNRPTGTASESVSIFQAQAEKMMKDSATKAAFTATASDIERYGDNYFGS
                     SLAVARNVLAANRGTRYIEVDHYGWDHHNLIYSNDNLYGRSLELDMAVSSLLEDLAAT
                     PGSASGKTLLDETLVMVMGEFGRTVGDLNPSSGRDHYPYVFSGLFAGGGVKGGRIIGA
                     TDDQGGGVQDFGWSVNRPIHMPDLVTTMFSVLGIDWTKTIADTPSGRIYRYADPEAVG
                     DDASYEISPLF"
     gene            2065..3999
                     /locus_tag="PKZ53_04845"
     CDS             2065..3999
                     /locus_tag="PKZ53_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNJ39794.1"
                     /translation="MKRLVLLLFSFAFVATVAYPALNTEPAKTHSAASSTSVQVRNRE
                     QAQKDARRKDPHAGLSVQIDTLGEAERATRAVTAKLPLRPLSGQPRIPASIQPAPDIS
                     RHLIDREIFGVLSQKGIKPAALATDEEFCRRITLDLTGRQPKPERLQQYVADTDPKKK
                     DKLITELMSSPFFVDRWTMWFGDLTRNYALTGPATDRNGMHEYLRQAVSTNKSVRDLA
                     TDVITYEGKASEGPGGFLVRPIFNTEIAQDAYDEMAAETMRTFLGTQAVCVSCHDGSG
                     HLEQVNLYFSTRQRSQYWGLAAFFSQTTFRTNTELNEFILATDRRGAYNADTDNGMRP
                     PRFGGRIEPSFELFRDGKPNAGEDLRKAAARLITSDRQFARAFVNRVFAHFFTVGLVD
                     PVDGFDLARLDPKNPPPAPWELQPSNPVLLEELAKYFEAKNYDFRSLITLMVTSKAYA
                     LSSQYDETQWKPEYARLYARKLVRRLQAEEVLDAMCDATQKPALYPARGYSQGFTSAM
                     SLPGVDEPSYIRNGPRPTDDPYTVYTFLGSFGRGDRNTTPRTNNGSIVQALSLFNSEM
                     IINRIEDRSSLAKLTEGALDRKEITPQVAIERIYLNTIARKPTTEEIAALVAPVNSKQ
                     LTLEDLQWALLNRVDFVYNY"
BASE COUNT          994 a         1139 c         1149 g          977 t
ORIGIN      
        1 gcagatccgt ccttgggatt tccgggtaag aggatcgggt ggccaacggc cgcaaaggga
       61 gtaccttgta gctcagcatg ccccgccgga agcgctcggg tggctccctt acgatggacc
      121 cataacgatt ggttgttgag ctgctcgcga cgcgcaatgt tgtgacttat gaaatagacg
      181 agttcaccct gtgctcctgg taaaacctcc tgaaaggcct cgagcacgag tgcattaata
      241 agcaagtggt tcagcgttgc gaagtttgcg ccgagcgaca tgtcgtcgat gtacgtatca
      301 gcctctggag taccaagggg cgcatagacg agttcacgat ccccacccgg aaacggggtg
      361 cacgtatcag caaacttact caaccaacga cagtttcttc cacttcaatc ctgaacccaa
      421 aaccgcaggt tcccggtata accccccagg gattacccgc cggtctcccc acggctttac
      481 cgcgtgtgaa gtgtgtgaaa tgtccagcaa cggatgaagc cgaagctttg ccgccggctc
      541 tgaaatcgga aggaatcccc gcatttcccc caatgattac gacaccagcg atgctccggc
      601 caatcccacc gaagtccggg catcagcgat tagatgtacc tgtacgagga ggcacagtat
      661 ggccaaacgg gcgtcaaacg agccctgggt tttagaacga cgggcatttt tgcaactcgg
      721 tgctgccggg ttgacttcat tgctggtggg atggcccaac ccagccaaag ccgaaaccct
      781 ttcccgtctt gcaacgggag tccgagcggt aactccgcgc aattccgcac ggaattgtat
      841 tctggtaatg ctgcgtggtg gtcccagcca cgtggatatg ttcgatttga aaaaagggcg
      901 ttggacgcca aatgagttgg gtgttgaacg cctgtcaaat ggcattgact ggccgaccgg
      961 cattttgccg aggctcaaaa cccagctcaa taaatgctcg atcattcgtt cgctccagca
     1021 ttcagaagtt gtccatgaac gggctgagta ctttgttgag accgggcggc ggctcaatcc
     1081 aggcttgcgc aatgaagttc cacacctggg agctgttatt tcgcttgaat ctgaagccca
     1141 gcgcaccgcc caggatgtgt tcccatcctt tatgctgttt aactatgggt tttcctattc
     1201 caacaatgga ttcctttcgg ccacgcatgc cccgttcaac gtgttttacc cggcgtatgg
     1261 aattccaaac ctggatccac cagatggacc ggaaagtttt aatcggcggc gggccgcact
     1321 ccagtttgta gatggcattg ggaatcggcc aaccgggact gcttcagagt cggtttccat
     1381 ttttcaggcc caggctgaaa aaatgatgaa agattcggcg acgaaagcgg cctttaccgc
     1441 aacggcctcg gatattgagc ggtatggcga caactatttt ggcagctcgc tggcggtggc
     1501 acgcaacgtt ttggcggcca atcggggcac gcgctatatc gaagtggatc actatggctg
     1561 ggatcaccac aatctgattt acagcaatga caacctgtat ggccgaagtc tcgaattgga
     1621 catggctgtc agttccttgc tggaagatct ggccgcgact ccggggagtg ccagcgggaa
     1681 gaccctgctg gatgaaaccc tggtcatggt gatgggtgaa tttggccgaa cggtgggtga
     1741 tttgaatcct tcctcagggc gtgaccatta cccctatgtc ttcagcgggt tgtttgcagg
     1801 tggcggcgtc aagggaggcc gtatcatcgg agccaccgac gaccagggtg gtggagtgca
     1861 ggattttggc tggtcggtca accgaccgat tcacatgccg gatctggtaa ccaccatgtt
     1921 ttctgtgctg ggaattgact ggacaaaaac cattgctgat acaccttcgg gtcgcatcta
     1981 tcgctatgct gacccagaag ccgtcggcga tgatgccagc tatgaaatca gtccgctgtt
     2041 ttgaggggga ggggaaataa caatatgaaa cgattggttt tactcttatt cagttttgca
     2101 tttgtggcaa ccgtggccta tccggcgctc aacacggaac cagccaaaac ccattcggcg
     2161 gcttcctcaa ccagcgttca ggtccgaaat cgtgaacagg cccagaagga tgcccgtcgc
     2221 aaagatccac acgcggggct ttcggtccag attgatactc tgggcgaagc cgaacgtgcc
     2281 acccgagccg tgaccgccaa actgccactt cgcccgcttt ccgggcaacc ccgaattcca
     2341 gcttcaatcc agcccgcgcc tgacatttca cgtcatctga ttgatcgtga aatcttcggg
     2401 gtgctttctc aaaagggaat caaaccggcg gcgttggcga cagatgaaga attctgccgg
     2461 cgtatcacgc tcgacctcac cgggcgccag cccaaacctg aacggttgca gcagtatgtg
     2521 gcggataccg atccaaagaa aaaagacaaa ctgattaccg aactgatgag cagtccattt
     2581 tttgtggacc gctggacgat gtggtttggt gatttgaccc gcaactatgc cctgacggga
     2641 cctgcgactg atcgaaacgg catgcacgaa tacctccgcc aggctgtttc gaccaataaa
     2701 tcggtgcgtg atctggccac tgatgtgatt acctatgaag gcaaggcgtc ggaagggcca
     2761 ggtggatttc tggtacgccc gattttcaat actgaaattg cccaggatgc ctatgatgaa
     2821 atggccgccg aaacgatgcg gacatttctg gggacacagg cggtctgtgt atcgtgccac
     2881 gacgggtcag ggcatctgga acaggtcaat ttgtattttt caacccgtca gcggtctcaa
     2941 tattggggac tggcggcgtt cttttcgcag accacgtttc gaaccaacac cgaattgaat
     3001 gaattcatcc tggcgactga ccgtcggggt gcctataacg ccgatactga taacgggatg
     3061 cgaccgcctc gctttggtgg acgcattgag ccgtcatttg aacttttccg cgatggcaaa
     3121 ccaaatgccg gtgaagatct gcgcaaagcg gcagcccggt tgattacctc ggatcggcag
     3181 tttgcccggg cgtttgtcaa tcgcgtgttt gctcatttct ttactgttgg tctggttgat
     3241 ccggttgacg gatttgacct ggcccggctt gatccaaaga acccgcctcc ggcaccgtgg
     3301 gaattacaac cgtcaaatcc ggttttgctc gaagagttgg ccaaatactt tgaggccaaa
     3361 aactacgatt tccggtcatt gattaccctg atggtcactt caaaagccta tgcgttgtca
     3421 tcgcaatatg acgaaacgca gtggaagcct gaatatgccc ggctctatgc ccggaaactg
     3481 gttcgtcggt tgcaggctga agaggtactc gatgcgatgt gtgacgccac acagaagccg
     3541 gcgctctacc cggcacgggg ctatagtcag ggctttacct cggcgatgtc gttgccaggt
     3601 gtggatgaac caagctacat ccgcaatggt ccacgaccaa ccgacgaccc ttacacggtg
     3661 tataccttct taggctcgtt tgggcgcgga gatcgcaaca ccaccccgcg caccaacaat
     3721 ggttcgattg tgcaggcatt gtcactgttt aacagtgaaa tgatcatcaa tcggattgaa
     3781 gaccgatcct cgctggcaaa attgacggaa ggcgcccttg atcgcaagga aatcaccccg
     3841 caagtcgcca tcgaacggat ttatctcaac accattgccc gcaaaccaac gaccgaggag
     3901 attgcggctc tggtggctcc ggtgaattca aagcaactca cactggaaga cctgcagtgg
     3961 gcgctcctca accgggtaga ctttgtgtac aactattaga aaaaccaggg ctgaacaatt
     4021 ctcgggctca aggctgaaca attcttgggc tcagggcttg gggctgaaaa aaacgggctg
     4081 aagagaatgg gtaaacccac actcttcagc ctgttctttt ttcaatccga aggaatgatc
     4141 ccaacacatt ggctactgac gattggcttc aaactggttt atcattcgtc attccccaaa
     4201 tttcacctat ttcccaatct tgattcctat ctggccgatc aaggacaaac aggacaagc
//
LOCUS       DAOQDO010000223         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14258_length_4253_cov_3.740591, whole genome shotgun sequence.
ACCESSION   DAOQDO010000223 DAOQDO010000000
VERSION     DAOQDO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14258_length_4253_cov_3.740591"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            587..1489
                     /gene="larE"
                     /locus_tag="PKZ53_04850"
     CDS             587..1489
                     /gene="larE"
                     /locus_tag="PKZ53_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNJ39795.1"
                     /translation="MSIAALGECSTAETSPLSLSAETKERQLRDWFADHAPVLIAFSG
                     GVDSSYLSFIAHQELGDRALSVTAISPSFPQDQHQEIDRMVADWKLYHRWIHTQEMER
                     EQYRSNPVNRCYFCKNELFTHLIEIAREQQFTTICDGNNADDTGDYRPGMQAGRELGV
                     RSPLIEAGLTKAEIRERSRVWKLPTAEKPASPCLSSRIPYGMPVTIQKLSAIERGERA
                     IRELGFSVVRLRHHGDIARIEISAPELPRALDPVMAQHLVAVLKPLGFTYIALDLEGF
                     RSGALNATLPFAPKPDTSQPLNDD"
     gene            1621..2901
                     /locus_tag="PKZ53_04855"
     CDS             1621..2901
                     /locus_tag="PKZ53_04855"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNJ39796.1"
                     /translation="MSSAFVDDAKNIKEANPFESMMSRFDVAAKLLNLDPNIYRILRC
                     PNREMTAYIPTMMDDGHYEVFVGYRVQHNFARGPAKGGIRFAPDVSLDEVRALAAWMT
                     WKCAVVNVPFGGGKGGIICDPSKMSMTELERMTRRFTSELIDIIGPERDVPAPDMNTN
                     EQVMAWLMDTYSMHARHTVNAVVTGKPIELGGSKGRREATGRGLLFVIEEACKKFKLK
                     PEETRVVVQGSGNVGGIGAQLLQEKGFKVIALSEIKNGIYNPNGLDVNAALDYLKKHR
                     TFEGFEGGEIISNSDLLELECEVLMPAATENQITSKNADRIKCRIMAEGANGPTTAAA
                     DDILQSKGVFVIPDILANAGGVTVSYFEWVQNRMGFFWKEDVVNERLRDTMVDSFHEV
                     VRFSEERNVNMRIAAYMLAIDRVAYETKMRGIYA"
     gene            3177..>4253
                     /locus_tag="PKZ53_04860"
     CDS             3177..>4253
                     /locus_tag="PKZ53_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39797.1"
                     /translation="MKRFAQKLAVLVLGFLVCCTCVTAQETQREKNRSTQATVTGGTG
                     LFSVYDASTLKRGEFNVSYFYNNFDRDPGDVDISQNILNVGVGLTDRIELFASSIFRQ
                     QLSTSDVTGISGFYLPNVVFPGRFATATPTGSVTDPPPFVTYPGFVNALGQPINGATV
                     GGILPGAPGTFGTVPGYLNDYPFFGRSKHKVGNTTVGMKYRFNSQDAPIGLALIGFVD
                     IPSFTADSVFRDGLGGGIVSGSGAGDIDFGGILAVTPRFKLATVSMNFGLVKTGDPED
                     RFTFIDRRNKAIASVAVDIPFNDRVQFVSELVSTNYFGSGTPNLNPVNPLDVTVGARF
                     VPFGKNHKTFFSFGGAYRYMLTNSN"
BASE COUNT         1011 a         1119 c         1096 g         1027 t
ORIGIN      
        1 aaataccgcg cacaaaccca cgggggtcag ccgcaaattc cctggcgctg gatgaaacaa
       61 tcccgaaaaa tcgcgtgacc agcgattcct ccgaaagtcc agaaaagctc ggtggttcaa
      121 ttgaatgatt cattggattg agtgagttat tgagatggtt aatgtcttgc acagccaaaa
      181 atcctccgtc gagcaggcta ttctatgagg atgagatgcc tcacaaacgt ggcgagttac
      241 atatggttgc aactttttcc aggtaaatgc gaggcgaaac gatctgattg ctggaacttt
      301 tcaggtactt gctgcagatt ttcagcacct tgtgatgtga aatcgcagta acaggttgct
      361 gcagcgaggg tgatcctgat cgcccatcat acacaggggg aaccttctaa gcaactcagg
      421 ttgtgcaagt tagtgccaat ttacgccaag ccaagacttg ccttgccgca ccccttgcag
      481 tatgatggcg acgctttcca gcaggcgttg cagttccaaa cagtttctca cgttttttca
      541 cattcaatca cggctcacat caaccgacgg tttttatcta ccccctatga gcatagctgc
      601 cctgggtgag tgctccacag cggagacgtc accgctttca ctctctgccg agaccaaaga
      661 acgccaactg cgagactggt ttgccgacca tgctccggtg ctgattgcgt tttccggcgg
      721 tgtggacagc agctatttga gttttattgc tcaccaggaa ctgggtgacc gggccctgtc
      781 ggtaacggcc atcagcccga gttttcctca agatcaacat caggaaattg atcggatggt
      841 tgccgactgg aagctttacc atcgctggat tcacacccag gaaatggagc gcgagcaata
      901 ccggtccaac ccggtcaacc gctgctattt ctgcaaaaat gaattattta cccatctgat
      961 tgagattgct cgcgagcagc agttcacgac catttgtgat ggcaataatg ccgacgacac
     1021 aggtgattac cgccctggaa tgcaggctgg tcgcgaactt ggggtgcgca gtccattgat
     1081 cgaagcaggc ctgaccaaag ctgaaatccg cgagcgctca cgggtgtgga agttgccaac
     1141 ggcggaaaaa ccagcctccc cctgcctttc ctcgcggatt ccctatggca tgccggtcac
     1201 gatccagaag ctttcagcga ttgagcgcgg cgaacgtgcc atccgtgagc ttgggttttc
     1261 ggttgttcgc ctgcgccatc acggcgacat cgcccgcatt gaaatcagtg cacccgaact
     1321 cccacgggca ctggatccag tgatggccca gcatctggtg gctgtcctca agccactggg
     1381 atttacctat attgccctcg atttggaagg gtttcgctct ggtgctttaa acgcaaccct
     1441 gccgtttgcg cccaaaccag acacctccca acctctgaat gatgactgac ttcaaggctg
     1501 tttatcattc acgctttgct ctgtcctttg tttttgctca ccggttgtcg gctgagacaa
     1561 cccgcttttt tcaacatttt ttattctgat ttcttggttt tgaacccaag gaggaacccc
     1621 atgtccagtg cttttgtaga cgacgcaaaa aatataaagg aagccaatcc gtttgaatcg
     1681 atgatgtcac ggtttgacgt agcggccaaa ctgctcaatt tggatccgaa tatctaccgc
     1741 attttgcgtt gccctaaccg cgaaatgacg gcctatattc caacgatgat ggatgatggc
     1801 cactatgaag tgttcgtcgg ctatcgcgtt cagcacaatt ttgcgcgtgg accagccaaa
     1861 ggcggcatcc gctttgcccc agatgtttcc ctggatgaag tgcgcgcact cgcggcatgg
     1921 atgacctgga aatgcgcggt ggtcaacgtc ccgtttgggg gtgggaaagg tggcatcatc
     1981 tgtgacccgt ccaaaatgtc aatgaccgaa ctggaacgca tgacccgccg cttcacctca
     2041 gaactgattg atatcattgg cccggaacgt gacgtcccgg caccggatat gaacaccaat
     2101 gaacaggtca tggcctggtt gatggatacc tattccatgc acgcccgcca caccgtcaat
     2161 gccgtggtca ccggaaaacc aatcgaactg ggtggctcca aaggccgccg cgaagcgact
     2221 ggccggggat tgctctttgt gatcgaagaa gcctgcaaga aatttaagct caaacccgaa
     2281 gaaacccgcg tggtggttca gggttctgga aacgtgggtg gcattggggc ccagttgctt
     2341 caggaaaaag gctttaaagt cattgccttg tctgaaatca aaaacggtat ctataacccc
     2401 aatggcctgg atgtaaacgc cgcgctcgac tacctgaaaa aacatcgaac gtttgaaggg
     2461 tttgaaggtg gcgaaattat ctcaaacagc gacctgctcg aactcgaatg cgaagtattg
     2521 atgcctgccg cgacggaaaa ccagattacc tccaaaaacg ccgaccgcat taaatgccgc
     2581 attatggccg aaggcgccaa cggtccaaca accgcggcgg ctgacgacat tctgcaaagc
     2641 aaaggcgtgt ttgtgattcc ggacattctg gccaacgctg gtggtgtaac ggtttcctac
     2701 tttgaatggg tgcaaaaccg gatgggcttc ttctggaaag aagatgtcgt caatgaacga
     2761 ctccgtgaca cgatggtgga ttcattccac gaagttgtgc gcttctctga agaacgcaac
     2821 gtcaatatgc gcatcgcggc ctacatgctc gccattgatc gtgtggccta tgaaaccaag
     2881 atgcgcggga tctacgctta aagacagcgg gctgaaaaaa ccagggctca gggctgaaaa
     2941 accagggctc agggctgaaa acatcgggct cagggctgaa aaaccagggc tcagggcttg
     3001 gggctgaaga caatgggttc aatacctggc ccaacttctt cagcccaact tcttcagccc
     3061 gatttcttca gcccgatttc tttaaccctg gcgtctcaaa tccagctctt tccaaccagg
     3121 tgggatattc gcaaacgaat gtcccacttt gtttttgaac gacaagaggg aatgttatga
     3181 aacgatttgc tcaaaaactg gctgtattgg ttttaggctt cctggtctgt tgtacctgcg
     3241 tgacagcaca ggaaacgcaa cgtgaaaaaa accgcagtac gcaggcaacc gtgacgggcg
     3301 gaacgggatt gttttccgtg tatgacgcct caacgttgaa acgcggtgaa tttaatgttt
     3361 cctactttta caacaacttt gaccgtgacc caggcgatgt tgacatcagt cagaacatcc
     3421 tcaacgtcgg cgtgggtttg acggatcgaa tcgaactgtt tgcctcatct atctttcggc
     3481 aacagctttc aacctcggac gtcactggaa tcagtgggtt ttatcttcca aacgttgtgt
     3541 ttccaggacg ctttgcgaca gccactccga cgggatccgt taccgatcca cctccttttg
     3601 tgacctatcc ggggtttgta aatgctcttg gccagccgat caacggggcc acggtcgggg
     3661 gaattttacc cggcgccccc ggcacatttg ggacagttcc gggctatctc aatgactatc
     3721 cattctttgg acgcagcaag cacaaagttg gcaacaccac cgttgggatg aaataccggt
     3781 ttaatagcca ggatgcgcca attggcctgg ccctgattgg gtttgtggat attccatcct
     3841 ttaccgctga ttcagtgttt cgcgatggat tgggtggtgg aatcgtcagc gggagcgggg
     3901 ccggagacat tgattttggc ggcatcctgg ccgtcacgcc acggtttaaa ctggccacgg
     3961 tcagtatgaa ctttggcctg gtcaaaaccg gtgaccccga agaccgcttt accttcattg
     4021 accggcgcaa caaagccatt gcctcggtcg cggtggatat tcccttcaat gatcgggtgc
     4081 agtttgtatc ggaactggtt tcaaccaatt actttggcag cggaacgcca aaccttaatc
     4141 cggtcaaccc gctggacgtg acggttggtg cccgctttgt gccgtttggc aaaaaccaca
     4201 aaacgttctt cagctttggc ggtgcgtatc gctatatgct gaccaattcc aat
//
LOCUS       DAOQDO010000224         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14284_length_4249_cov_3.989986, whole genome shotgun sequence.
ACCESSION   DAOQDO010000224 DAOQDO010000000
VERSION     DAOQDO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_4249_cov_3.989986"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2538
                     /locus_tag="PKZ53_04865"
     CDS             157..2538
                     /locus_tag="PKZ53_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39798.1"
                     /translation="MSRTALKFFWIVLCLFGMSTMAQAQDFEVLRYDVTASLQPVANA
                     VDVKATLSLVNQTTQGQSATDLILRLNKDAKVTALLLNGATTEFTQKEDPRLTGVSIV
                     SAQLVKSVPPAGKLEAVLQYTLTVKESNQYSAITPGDCLLLPESYWIPVVHTPFTSHG
                     ADTAPYTLTVTLAGDGDILSEGERKGDGKTVMFTQTLNSQPWLMVGAPGKLVTRSITP
                     AGAAPITLTTISQVGSAGTEAQVQRILDEMEKIVGFYVQTLGPSPTNQFVVASNFREV
                     TYISPGTILVGNAVFRRDRVEAETIEYLSRAVARTWIGGKLRVRGRGLGIVQDALPSF
                     LSGLYFESRFGADAGQQFWARRVRSYAPLALARTDAMLMAQVPLDSDYFTSILNKGAL
                     VFRLIDWQFGKNTVVTAVKTILTGTTMDPLTVEGLRSTLIGPDKNANQPLQRFLKQWW
                     DEIVEPDLIIGLPKKNETGTAWTCVLRNLGTGDVTVPVVAITGKGETLTTKAAIPSKG
                     LGSVEFQTAEEVVRVEIDPDKLYPQTKFELDPNSSQFDNDSRPPRQYSFSLFLEANQL
                     FEQKEFQKAEAKLLQTVVQEPGYASAQMLLARVELALGKSEATQSALQKALDVKPTPL
                     YVTGWSAIVEGELALTRKDFKAAVEAYRRADGANAELACTLAAHKGLIESETQLQQIS
                     MPDESVKAYFSQLEKAVQSKTASVIEALVIKAELPKFVKGLALTKPDVWKTEVIRASQ
                     LDTQHVAVDVNLEALTTDLITGEKKDQKGSAVFILRKTQSGWVLARIDLFTVK"
     gene            complement(2701..3414)
                     /locus_tag="PKZ53_04870"
     CDS             complement(2701..3414)
                     /locus_tag="PKZ53_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ39799.1"
                     /translation="MSQKVAIVTGSTKGIGRAVAEYLLNDGYAVVISARNLAEVDTTV
                     AEFSQVPGRQVSGTSCDMRDYTQVESLVQHAIETFGRLDVLVNNAGVGRFATVEAMSP
                     DIWREVIETNLNGVFYACHAAIPHLRASGGGYIFNISSLAGKNAFPQGGAYNASKFGL
                     NGLSEVLFQEVRYDNIKVSYIMPGSVTTYFNGHVPSPEDDWKLAPEDIAQVVIDLLHH
                     HPRSLPSAVEIRPAKPKKK"
     gene            complement(3508..>4249)
                     /locus_tag="PKZ53_04875"
     CDS             complement(3508..>4249)
                     /locus_tag="PKZ53_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39800.1"
                     /translation="PSKQLIGFDIDPSFGSLVAFGVSESRYLISPLRLDEVGVIRPYS
                     GDQVAFIELLSGDVLGQNTKVAFDLYGSQFMVGSSSSGRVYVYRMDTQTDGLPISWIT
                     IDDLTLDPAEATDQGIQLQFNQATRKAFLTRPGHLTRPGHLTRPGHIAMFGLDTDAKF
                     SQVNTFGSDDTIQDAHLSPDGNTIYVLSAMGSILEYTTDGSLTSAHPISVAGMKLTVG
                     NDGALGVLGWGGTEENSTSQVQVVSAQK"
BASE COUNT         1116 a         1045 c         1049 g         1039 t
ORIGIN      
        1 gttcttggtt cttggttctt ggttcttggt tcttggttct tcttcgaaag tattggttcc
       61 aaagcaccag gtaccaagta ccaagtacta gccactaacc accagaaggt ttcttcattc
      121 tcccaggcca ttttcacccc ataagagaaa gaatttatgt cacgtactgc tctcaaattt
      181 ttctggatcg tgctctgtct ttttggaatg agcacaatgg cacaggctca agattttgag
      241 gttcttcgct atgacgtcac agcctcgctt caaccagtcg ccaacgcggt tgacgtcaag
      301 gcaaccttaa gcctggtcaa ccagaccaca caggggcaat cagcgacgga cttgatactt
      361 cgtctgaata aagacgccaa agtgacagcc ctcctgctca atggggcaac aacggaattc
      421 actcaaaagg aagaccctcg cttgactggt gtgagcattg tcagcgccca actggtcaaa
      481 tcagttccac cagccggaaa acttgaagcg gtgctgcagt acaccctaac ggtcaaagag
      541 agtaatcagt attcggcaat tacaccgggg gattgtttat tgctcccgga aagctactgg
      601 atcccagtgg tgcatacacc ttttaccagc catggtgctg acaccgcccc ttacaccttg
      661 accgtaacgc tggcaggtga tggagatatc ctgtctgagg gtgagcgcaa gggcgacggg
      721 aaaacggtga tgtttaccca aacacttaac agtcaaccct ggttgatggt tggagcacca
      781 ggaaaacttg tcacccggag cattactcca gccggagccg caccaattac actgacgaca
      841 atctctcagg ttggatcggc tggcaccgag gcgcaggttc aacgcatttt ggacgagatg
      901 gagaaaatcg tcgggtttta tgttcaaacc cttggcccat caccgacgaa tcaatttgtc
      961 gtggcttcaa attttcgaga agttacctat atctcaccgg gaacgatttt ggttgggaat
     1021 gcggtgttcc gccgcgatag agttgaagca gaaacaatcg agtacttaag ccgggcagtg
     1081 gctcggacct ggattggcgg aaaacttcga gtccggggac gaggattagg tattgttcag
     1141 gacgcgttac caagctttct gtcaggactt tattttgaaa gtcggtttgg ggctgatgcg
     1201 ggtcagcaat tctgggcccg gcgggtacgc agttatgctc cgctggcctt agccaggaca
     1261 gatgccatgc tgatggcgca ggttccgctc gattcagatt acttcaccag tatccttaat
     1321 aaaggggctt tagtttttcg tctgattgac tggcaatttg gcaaaaatac tgttgtaact
     1381 gcggttaaaa ccattctgac tggcaccacg atggatccat tgacagtcga agggctccgg
     1441 tcaactctga ttggcccaga taaaaacgcc aaccaacctt tgcagcgatt tttgaagcaa
     1501 tggtgggatg aaattgttga acctgacctg atcatcggcc ttcccaaaaa gaatgaaacc
     1561 ggaactgcct ggacctgtgt actccgtaat ttggggactg gagatgtaac agttccagtc
     1621 gttgccatca ccggtaaagg tgagacattg acaacaaagg cggccatccc gtcaaaaggg
     1681 cttggatcgg ttgaatttca gaccgcggaa gaggtagttc gggttgaaat tgaccctgat
     1741 aagctctacc cacaaaccaa atttgagcta gaccccaatt catcgcaatt tgacaatgat
     1801 agccggccac cccgtcaata ttccttttcc ctctttttgg aggctaacca gctctttgaa
     1861 caaaaagaat ttcaaaaggc cgaagccaaa ttgttgcaga cagtcgttca agaaccaggc
     1921 tacgcttcag cgcaaatgtt gcttgcccgg gtggaactgg cgttggggaa atctgaggcg
     1981 acccaaagtg cgcttcaaaa ggcgctggat gtaaagccaa cgccgctcta tgtcaccggg
     2041 tggagtgcca ttgtagaagg tgagttagcc ctgacccgca aagactttaa ggccgcagtt
     2101 gaagcctatc gtcgggcaga tggggccaat gccgaactgg cctgtaccct ggcagctcac
     2161 aagggactta tagagagtga aacccagctt cagcaaatca gcatgccgga tgagtcagtc
     2221 aaagcatatt tcagccagct tgaaaaagcg gttcaaagca aaacggcatc ggtgattgag
     2281 gcacttgtca tcaaggcaga attaccgaag tttgtgaaag gtctcgctct taccaagcct
     2341 gatgtctgga aaaccgaggt gattcgggcc agtcaacttg atacccaaca cgttgccgtt
     2401 gatgtgaatc ttgaagccct cacaactgac ttgatcactg gtgaaaagaa agatcagaaa
     2461 ggaagtgcgg tttttattct tcggaagacc cagagtggat gggttctggc acgaattgat
     2521 ttgtttacag tgaagtaatt ggagtcagtc gtcagtcgtc gagtgttggg gttttgagtt
     2581 gcctgtcaag cgtatttggt tctttttgag taatttgcaa cgagttcttt caagatcttt
     2641 gcagactaaa ataccagggt ttggtctact gacttatcaa cgactgacta ctgactggta
     2701 ttatttcttc tttggttttg caggccggat ttcaaccgcg ctgggaagac tgcggggatg
     2761 gtgatgcagc aagtcaatga ccacctgggc aatgtcttcc ggcgccagct tccaatcatc
     2821 ctcgggtgac ggcacatgac cattaaaata ggtggtcacg cttccaggca taatgtaact
     2881 gaccttgata ttgtcatatc gaacttcctg aaacaggact tcgctcagcc cattcaatcc
     2941 aaattttgaa gcattgtagg cgcccccctg cggaaaagca ttttttccag ccaggctgct
     3001 gatattgaaa atgtatcctc caccactcgc tctcaagtgc ggaatggctg cgtggcaggc
     3061 ataaaacacc ccattgagat tggtttcaat cacctcgcgc cagatatcag gcgacatggc
     3121 ctccactgtg gcaaatcggc caaccccggc gttattgacc aggacatcca accgaccaaa
     3181 tgtttcaatc gcgtgctgga cgagcgattc aacctgggta tagtcacgca tatcgcatga
     3241 cgttccgctc acttgccgac ctggtacctg gctgaattca gcaacagttg tgtcaacctc
     3301 agccagatta cgagcactga taaccacggc atatccatca ttgagcaaat attcagcaac
     3361 tgcccgtcca atgcctttgg tgcttccggt cacaatggct actttttgag acatagttca
     3421 cgatcctctg aaaaaagagc atccgaaaag cgaatcgggc ggttacccgc ccgattcagt
     3481 gaaaactggc cttgattcaa gcaataatca tttttgggct gatacaactt gaacctggct
     3541 ggttgaattt tcttccgtgc caccccagcc taatacgccg agtgcaccat cattgccgac
     3601 tgtcagtttc ataccagcca cactgattgg gtgggctgat gtcagactgc catcggtggt
     3661 gtactcaaga atgcttccca ttgccgaaag aacatagatg gtatttccat cgggtgagag
     3721 atgcgcatcc tgaatggtgt catctgaacc aaacgtattg acctgactga acttcgcatc
     3781 ggtgtcaaga ccaaacattg cgatatgccc tggacgggtc agatggccag gacgagttaa
     3841 gtgcccagga cgggttaaaa aggctttacg ggttgcctgg ttaaactgga gctgaatccc
     3901 ttgatcagtg gcctcagccg gatccagagt caaatcatca atggtaatcc aggaaatcgg
     3961 aagtccatcg gtttgggtgt ccatccgata gacatagacc cgtcctgatg aacttgagcc
     4021 aaccataaac tggcttccat acagatcaaa tgccaccttt gtattttggc caagcacatc
     4081 accagacaac agttcaataa aagcaacctg atctcccgaa tacgggcgaa tcacaccaac
     4141 ctcatctaaa cgcagaggag aaatgaggta gcgactctcg gaaacaccaa aagcaacaag
     4201 cgatccaaag gatggatcaa tgtcaaagcc gatgagttgc ttacttgga
//
LOCUS       DAOQDO010000225         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14331_length_4242_cov_2.574397, whole genome shotgun sequence.
ACCESSION   DAOQDO010000225 DAOQDO010000000
VERSION     DAOQDO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14331_length_4242_cov_2.574397"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKZ53_04880"
     CDS             complement(<1..194)
                     /locus_tag="PKZ53_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39801.1"
                     /translation="MTNLTLTRLTHNFSQAVRSGWRFGYVTLLLSPFLISFLRDQRRF
                     VKWGAPRDLSLAAHHRRARWL"
     gene            complement(323..1564)
                     /locus_tag="PKZ53_04885"
     CDS             complement(323..1564)
                     /locus_tag="PKZ53_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39802.1"
                     /translation="MIFRLRTRQRFYLITAAILGLLSLALLYRFSYWIGGLGLLVTAA
                     VYCCSYLECIIFDGNALTRTGPITWVLAQLSNQNWYLPIGEIEMIATESTRLRFPPDR
                     IRYRYRTLVLGSHAEFVLNSSEPGYPQLAKALFCSVGEDKLDPRSSELLYYFDTVEGY
                     ESFDFPQDHISKLPTQLLRSIANGLRLSGQMRQALNCFMVAYQREPNNPHLLYEMARF
                     LRSLAALDGPRLLTRSSACLRLAARISQNEPRLLERIGETYFERLEYDRASRCFARAL
                     ELDPNLFRAHVGMAEIALRSGKLAHVAHFYGTAAHSTLDAAQARLANREASYYGRLCS
                     DEDYLEAEINRITMLRHLRWIRTAAATVFFIALTAACLVSAFYAPLETLSWSFVISSS
                     FLWAVATLGLQWYNKRLDATV"
     gene            complement(1966..2760)
                     /locus_tag="PKZ53_04890"
     CDS             complement(1966..2760)
                     /locus_tag="PKZ53_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39803.1"
                     /translation="MVSHSKKLSKQELKREPAFNVWLRGIQHQVEDMGGNVVKLGLGV
                     LIALVVIGGGYAFYSSNRAKGEAAFAKALKIYQAEVQDPKAPNPTPPVPGKVVYQDEQ
                     KKYTEAATAFEQAAAYSAQRELSRYYVGLCRLKLDVPKGQQELRALCTGDGQINQMAR
                     IAVADSLAAGGQTDEAIKYYLELQGIWEKNQGVGVILPPEAIQFSIAKLYDAQGKTAE
                     AVQAYTKVITTNRSSRLAMESYGRLSALDPESARKMTPPKLNEEAI"
     gene            3467..>4242
                     /locus_tag="PKZ53_04895"
     CDS             3467..>4242
                     /locus_tag="PKZ53_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ39804.1"
                     /translation="MALVGDLKDLALVDIIQINCVGRNTARLTVHYPIGDGIFYFAEG
                     EVVDARLGNLVGTDAVYKALAYDQGSFRIDTGIPAPQRTIFEPWANLIMEGLRLLDEA
                     RAGLIPDHTGLVGISTPASTETQRVQNVYQTLVSDLTKIKGIDGAVAMARDGTVHSSL
                     NIKPSEKIGMMSALLVHMARQTSTPARVGPLQRTTISFGQRKSTIFDQDTYLVLIEYG
                     SAIRYETISPHIDRAFRKLAARSSGGTGALTGPLGGTGMLG"
BASE COUNT         1040 a         1033 c         1177 g          992 t
ORIGIN      
        1 agccagcggg cccgccgatg atgcgccgcg agggaaagat cacgcggtgc cccccatttg
       61 acaaagcggc gctgatcccg cagaaaactg atcaaaaagg gcgacaggag cagcgtgaca
      121 tatccaaacc gccacccaga tcgaacagcc tgggaaaaat tgtgggtcaa gcgggtcaat
      181 gtgaggttgg tcaccagaag tagatgcctc gcagaatgtg ggattcaccg gcgacctgaa
      241 ttgggaacgg ggatgaactc ttgacgtggt gccggatgga ccgccgtggc cagtcgcagg
      301 ctggcggctg gcggctttag cgtcacactg tagcatcgag gcgcttgttg taccactgaa
      361 gaccgagggt ggctaccgcc cataaaaaac tggaggaaat gacaaaggac caggacaggg
      421 tttcaagcgg ggcataaaaa gcactgacca gacaggcagc ggtcagggca ataaaaaaca
      481 cggtggcggc agcggttcgg atccagcgca ggtggcgcag catggtaatg cggttaattt
      541 cagcttccag atagtcttca tcactgcaga gccggccata ataagaagct tcacgattgg
      601 ccaggcgtgc ctgtgcggca tcaagtgtcg aatgagccgc tgtgccgtag aaatgggcaa
      661 catgggccag tttaccggat cggagggcaa tttccgccat tccgacatgg gctcgaaaca
      721 ggttggggtc aagttcgagg gcccgtgcaa agcaacgcga ggcccgatca tattccaggc
      781 gttcaaagta ggtctcgccg atgcgttcaa gtaatcgtgg ctcattttgc gaaatgcgag
      841 ccgccaggcg caggcaggcg cttgaccggg tgaggagccg ggggccatca agggctgcca
      901 gtgatctcaa aaaccgcgcc atttcataga gcagatgcgg gttattgggt tcacgctgat
      961 aggccaccat aaagcaattc aaggcctgcc gcatctggcc tgacagccgt aacccattgg
     1021 caatggagcg caggagctgg gtgggaagtt tggagatatg gtcttgtggg aagtcaaaac
     1081 tctcataccc ttcaaccgtg tcgaaataat agagtaattc gctcgaacgg ggatccagtt
     1141 tatcttctcc caccgaacaa aacagggctt tggcaagctg gggatagccc ggctcgcttg
     1201 aattaagcac aaattcggcg tggctgccca aaaccagcgt gcgatagcga tagcggattc
     1261 ggtcaggagg aaagcggagg cgggtggatt ccgtggcgat catttcgatt tcgccaatcg
     1321 gcaggtacca gttctggttg ctcaactgag ccaggaccca ggtgattggt ccggttcgag
     1381 tgagtgcatt gccatcaaaa atgatgcact caagataaga gcagcaataa acagcggcgg
     1441 tgaccagcag gcccaggcca ccaatccaat atgaaaagcg atacaacaga gccagcgaca
     1501 acaggccaag tatcgcagcc gtgatcaggt aaaatcgttg tcgagtacgg agacgaaaaa
     1561 tcaagtcgcc ctcaagtggt tcacagaaat tttggacttc gattccctgg acaccaaaac
     1621 cgatggatca cctctggtga cgaacctggt tgaattctga tttgccaggg gggatacaca
     1681 tgatggtcag gggtgcaaaa aacgcctgac cttcaggtct ttggcctcag ggaaggtgaa
     1741 tcagatttga atcaggttga ttttacaaaa agaggccatc actggctgtc tcatttttgt
     1801 aaaatttcaa gttgtctttc tgttcagact aaaatcttcg ggctgaagac tcgcaagctc
     1861 agggctgaag aaattgggct caaagtatac aaattatgtg aattcaagtg ggtttacccg
     1921 caacccgcag cccgtttttt agcccgccgt cttcagccct ggttcttaaa tggcttcttc
     1981 attcaacttc ggcggggtca tttttcgagc tgattctgga tccagggccg ataaccgtcc
     2041 ataggattcc atcgccagcc gtgaagaccg gttggttgtg atgactttgg tgtatgcctg
     2101 gactgcctca gccgtcttgc cttgcgcatc atagagtttg gcaattgaaa actggatggc
     2161 ttctggcggc agaatcaccc cgacgccctg attcttttcc cagattccct gcaattccag
     2221 atagtacttg attgcttcgt ctgtttgacc gccagccgcc agcgaatcgg ccacggcaat
     2281 gcgggccatc tgattgattt gcccgtcacc cgtgcagagt gcccgcagtt cctgctgtcc
     2341 ctttggaaca tcaagtttca gccggcaaag cccgacataa tagcggctga gttcacgctg
     2401 ggctgaatag gccgctgcct gttcaaacgc ggttgcggct tcggtgtatt ttttctgctc
     2461 atcctggtag accactttac cagggaccgg aggggttggg ttgggggctt tcgggtcctg
     2521 aacttcagcc tggtaaattt tgagtgcctt ggcaaaagca gcttcgcctt tggcccgatt
     2581 gctggagtag aaagcatacc cgccaccgat gacgacaagg gcaatgagca cgccaaggcc
     2641 caacttgacc acattcccgc ccatgtcctc aacttgatgt tgaattccgc gcaaccagac
     2701 attaaaagcc ggttcgcgtt tgagttcctg tttactgagt ttcttgctat gggaaaccac
     2761 tactttgcgc tcctcgattt cagaatgatt aagacattga ctccatatcg cagattttag
     2821 aactgacaaa agacaatatg ccttattttg aggttgccgg actcaggttc acgcaggctg
     2881 gattgacgag tgccagtcct ttttcagctt tttgagcctg aagtttacag ttccaggccc
     2941 gaaattctgc atcccagaac gagagggtat ctcgtggtgt gaaaaggata tggatatgaa
     3001 acctcaaaca tagtctgatg actttgccag tgtcaagcag gtgagtgcgt ggcgaggagg
     3061 gggaggaggt aggaacttgt ccaattgact gaagaggaag gtgaaaccga ttggcgtcgg
     3121 gggcgtgctg cccccgaaac cactcgcaaa ttgaataact tgaaatctga gatcgtttag
     3181 attgacactc tcaaagtaac tctgctagtt tagccagtga agtaacccct ttccgcctga
     3241 atgaatatct ctccaaattg agatctgtaa acaaactccg tcacctggtc aacctggctt
     3301 gggtgtgtgg tcagcgattt gacccacaag gaggagcgtt ttcatttcag gaagcgtttg
     3361 acaaacccaa ttctgctcgc gttcatctcc gaagttttac tgccgagacg gacgcatctg
     3421 atagaagtag ctgaaaaacc ctgtttgtga agtgtgagaa agtcttatgg ctttggtggg
     3481 agatctcaaa gatttagccc ttgttgatat tatccaaatt aactgcgttg ggcgaaatac
     3541 agctcggttg acagttcact atccaattgg cgacgggatt ttttactttg cagaaggtga
     3601 agtcgtcgat gcccgcctgg gcaatttggt tggaacagat gctgtatata aagccctggc
     3661 gtatgatcag ggatcttttc gcattgatac agggattcca gccccccagc gaacgatttt
     3721 tgaaccctgg gccaacctga ttatggaagg gctgcggctc ttggatgaag cccgagctgg
     3781 attgattcct gatcataccg gactggttgg tatctcgacc ccggcatcaa ctgaaacgca
     3841 gcgggtgcaa aacgtttacc agacactggt ttcagatctg actaagatca aagggattga
     3901 tggggcggtc gctatggcgc gggatggtac ggttcattcg tcactcaata tcaagccatc
     3961 tgaaaaaatc ggtatgatga gtgccctgct ggttcatatg gctcgtcaaa ccagcactcc
     4021 ggcccgggtt ggcccgctgc aacgcaccac catcagtttc ggtcagcgaa aatcaaccat
     4081 ttttgaccag gacacctatc tggttttgat tgaatatggc tctgccattc ggtatgagac
     4141 aatcagtccg catatagatc gggcgtttcg gaaactggct gcccggtcaa gtggcggcac
     4201 tggtgctttg accggacctc tgggcggcac cggaatgctg gg
//
LOCUS       DAOQDO010000226         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14339_length_4240_cov_4.397372, whole genome shotgun sequence.
ACCESSION   DAOQDO010000226 DAOQDO010000000
VERSION     DAOQDO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14339_length_4240_cov_4.397372"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="PKZ53_04900"
     CDS             <1..1831
                     /locus_tag="PKZ53_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ39805.1"
                     /translation="FSRAASGYPDTPLVRDAQLWAGKSAAQKLDLQALEQELKPLLDQ
                     GDGTALLLLAQTLETQGKSTEAIRAYQRIYFEAPHTNECTVAVSKLPLLGIPLDAPEM
                     MNARQWQIRADRFFENKQFVSAAEAYAQLEKLNPGALQSDQNRLRYGISLVTAQKYPA
                     AIPYLSQISSKNKELYPEALLYLAKAERRANLPSHVATTERFLSLYPKHPLAGDALAD
                     LAAYQEKRGQGTTYYARLMRDYPNEKAGDDYSFKRAWKAHEAKNYAAAVNFLIEHVGN
                     YPDSDFKGKSAFWAARDAERINDLERALILYDAVSKRYITSLYGSLATDGVKRIKAQS
                     NLKPAKPSPSSPLGRALNNIQPAQVPPETAGKEADSFLNRASDLRIIGLMDLAMTELE
                     AARNTAPRSKKINLEIARVNRDKGDTLAAMRSISRAHPDYLYYQENQLSREEWDLVFP
                     MVEWETIQREARANGLDPFIVAGLIRQESVFNPRAKSRANAFGLMQLLPSTGRMVAKK
                     SSVGAITEEQLYNPRLNIQLGTIYLSDMVRKFGRYEYAFAAYNGGPGRVSKWLQTLPS
                     DDLITWVESIPITETRLYVHGVLRNAAHYRRLYGEKNLNPQPE"
     gene            complement(2040..2357)
                     /locus_tag="PKZ53_04905"
     CDS             complement(2040..2357)
                     /locus_tag="PKZ53_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39806.1"
                     /translation="MSISVTDEGVTRILGNGKVESVTWDDLAEVAILTTSAGPFEEDV
                     YWLLIGKSGGGCAVPQSLDPQGEILKRLQTLPGFDNEAVIRAMGSVENATFVCWKAEQ
                     NEE"
     gene            complement(2547..>4240)
                     /locus_tag="PKZ53_04910"
     CDS             complement(2547..>4240)
                     /locus_tag="PKZ53_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HNJ39807.1"
                     /translation="MEEFGVEVLGVIYGLPTPTQSSIIIRLSGANAERTGVFAGMSGS
                     PVFINGKLVGAIAFSFPFSKEPLGGVTPIEEMLSIFETGESDVHTARGLFSFQDLATV
                     KPASVPVELSQRQTPVLIGADAAYTPSLAPLVGQTMMPIATPISVSGVPSDILAQYAP
                     QFEALGFRLVSGLSGAAPMGTGLKPFDDTTLLPGSTISVLLAGGDFSFAAGGTVTWRD
                     GNQVYAFGHPFLNFGATQFPMSEGSVVTVVPNINNSFKLTIPGPPVGMINQDRTTAIR
                     GQLGQAAPMIPITLNVTTSRGAERTYRFEVINDSFLTPLLVSLGVSSGLTATERALGD
                     LTLHMKGQIQLEGHAPIDLSNSFTSRLNPAGLIGLSVSQPLGVLLGTGFEDVTIKGVT
                     LSVIASDARTNGSLERIWVSKTKARRGETIEIQAFARNANGQTFVERIPIEIPVDARP
                     GMATISVGDGATIGQSERRTLPEPKDTAQLVRFINQLRKNNRLYVRLLRAEAGAVVNN
                     EEMPSLPPSMLATLSSDRVSSEYQPLKNSILVEKELPSARFLITGQQSISIEVVK"
BASE COUNT         1079 a         1132 c         1031 g          998 t
ORIGIN      
        1 gttctcacgg gcagccagtg ggtatccaga tacgccactt gtgcgggatg cccagctttg
       61 ggctggaaaa tcagccgctc agaagctcga cctccaggcg ctcgaacaag aattaaagcc
      121 attgcttgac cagggggacg ggacggccct cttgctcctg gcccaaacgc tggaaaccca
      181 gggaaaatca actgaagcca ttcgcgcata tcagcgaatc tacttcgagg cgcctcacac
      241 aaacgagtgt acggtggcag tcagcaaact cccgctcctt ggaatcccgc ttgatgcccc
      301 tgaaatgatg aatgcccggc aatggcagat tcgggcggac cgcttttttg aaaataaaca
      361 gtttgtgagt gctgccgagg cgtatgcgca gcttgaaaaa ctgaacccag gggcgctcca
      421 atctgatcag aaccggcttc ggtacggtat ttcactggtc acagcccaaa aatatccagc
      481 agcgattcct tatctcagtc agatcagttc gaaaaacaaa gaattgtatc cagaagcctt
      541 attatatctg gcgaaagccg aacgccgggc aaatctccca tcccatgtgg ccaccactga
      601 acgctttttg agcctgtatc ccaaacaccc actggcaggc gacgcactgg ccgatctggc
      661 tgcgtatcag gaaaaacgtg gacagggcac aacctattat gcccggctga tgcgtgacta
      721 tccgaatgaa aaagcgggtg atgactattc ctttaaaaga gcctggaaag cccacgaagc
      781 caaaaactat gcggcagccg tgaatttcct gatcgaacac gtcggcaatt atcctgattc
      841 cgattttaaa ggcaaaagtg cgttttgggc ggcacgtgat gctgaacgca tcaatgatct
      901 ggaacgggca ctcatactct atgatgcggt ttccaaacgc tatatcacca gtctctatgg
      961 gtcgctggcc actgatgggg taaaacgcat caaagcacaa tcaaacctca aaccagccaa
     1021 accttccccg tcctctccac ttggccgggc attgaataat attcaacctg cccaggttcc
     1081 gcccgaaacc gccgggaaag aagctgattc atttctgaat cgagcctcgg atctgcgaat
     1141 tatcggtctg atggatctcg caatgacgga acttgaagct gcgcgaaata cagcccctcg
     1201 ttccaaaaag atcaaccttg aaatcgcccg cgtaaatcgt gacaaaggtg atacgctggc
     1261 agccatgcgg tcaatctcgc gggcacaccc cgattacctc tattatcagg aaaatcaact
     1321 ctcccgcgaa gaatgggatc tggtgttccc aatggttgag tgggaaacta ttcaacgtga
     1381 agcccgtgcc aatggacttg atccatttat cgtagccgga ttgatccgcc aggaatcagt
     1441 tttcaatccg cgggcaaagt cccgcgccaa cgcctttggg ttgatgcaat tgctgccttc
     1501 taccgggcga atggtagcca aaaaatccag tgttggcgcc atcaccgaag aacaactcta
     1561 taaccccagg ctcaatattc aacttggcac gatttatctg tctgatatgg tgcgaaaatt
     1621 tggccgatat gaatacgcct ttgcggcgta taacggcggc cctggacgcg tttccaaatg
     1681 gctgcaaaca ctccccagcg acgacctgat tacctgggtt gaatcaattc caattactga
     1741 aacccggctt tatgttcatg gtgtgttgcg caatgcggcc cactaccgcc ggctctatgg
     1801 cgagaaaaat ctaaacccac aacctgaata gaatgaagaa tgaagaattg agaatgaaga
     1861 atgaagaaac tagttcttgg ttcttggtgc ttggttcttg gtgcttggtt cttggttctt
     1921 ggtgcttggt tcttggtgct tggttcttgg tgcttggaaa atattgattc ctaagcacta
     1981 agcacattct caattcttca ttcttcattc ttcattcttc attcttcatt cttcattctt
     2041 cattcttcat tctgctctgc tttccagcac acaaatgtgg cattttccac cgaacccatt
     2101 gcccgaataa cagcttcatt gtcaaaacca gggagtgttt gcaaccgctt taaaatctcc
     2161 ccttgtggat ccagggattg aggaaccgca catccaccac ccgacttccc aatcaacagc
     2221 caatatacgt cctcctcaaa cggcccggcg ctggttgtca gtatggcaac ttcagccagg
     2281 tcatcccagg tgactgattc aactttgcca tttcccagga tgcgggttac cccttcgtca
     2341 gtgactgaaa ttgacatggc agggtctgat tttctttgaa agagggattt aagccagttc
     2401 agcatagagc gtgaaggggg caaagaacgg cgagtagcga aataacgtcg tcgtgaacga
     2461 cacaaagttc aagattgaca ggtcaggact gggaatcaag cgtgaaggac ctggcgactt
     2521 cacgctttcc cccttcgctc tgcttgctac tttacaacct ctattgaaat tgattgctga
     2581 ccagtaatta aaaagcgggc tgaaggaagt tctttttcta ccagaattga gttcttgagg
     2641 ggctggtatt cgcttgaaac tcgatcactg ctcagggttg ccagcatgga aggtggcaag
     2701 gaaggcattt cttcgttgtt gactacggcc ccagcttctg cccggagcag tcgaacatac
     2761 agacggttgt tcttgcggag ctggttgata aaccgaacta actgagccgt gtcttttggc
     2821 tcggggagcg tccgccgttc gctctggcca atcgtggcac catcaccaac tgaaatcgtg
     2881 gccatccctg gtcgggcatc caccggaatt tcaatcggga ttcgttcaac aaaagtctga
     2941 ccgttcgcat tacgggcaaa agcttgaatt tcaatggttt ccccacggcg tgcctttgtt
     3001 ttgctgaccc agattcgctc aagtgacccg ttggttcggg catcactggc aatcacgctc
     3061 aaggtcacac ctttgatcgt gacatcttca aacccggtgc cgagcaagac cccaaggggt
     3121 tgggagacag aaagcccaat caatccagcc gggttgaggc gcgaagtaaa actattgctc
     3181 aggtcaattg gggcatggcc ttcgagttga atctggcctt tcatatgcag tgtcagatca
     3241 ccaagtgctc gttctgttgc cgttaaacca cttgaaacgc caaggctgac cagaagcggt
     3301 gtcagaaagg aatcattaat cacttcaaac cggtaggttc gttctgcacc ccggctggtt
     3361 gtgacattta atgtaatcgg aatcatcggt gcagcctgtc ccagttggcc ccgaatcgca
     3421 gtcgtccgat cctgattgat catcccgact ggaggcccag gaatcgttaa cttgaaagaa
     3481 ttgttgatat tgggaacaac tgtcacgaca gagccctctg acattggaaa ctgggtggca
     3541 ccaaagttta agaagggatg accgaaggca tacacctgat tgccatcgcg ccaggtcacc
     3601 gttccgcccg cagcaaagct aaaatctcct cccgccagca aaacgctgat cgtggagcca
     3661 ggcagcaacg tggtgtcatc aaacggcttt aagccagttc ccatcggggc cgctccgctc
     3721 aaaccggaaa ccagccgaaa ccccagggct tcaaattgcg gtgcatactg ggccaaaata
     3781 tcactgggaa cacctgaaac tgaaattgga gtcgcaatcg gcatcatggt ttgccctacc
     3841 agcggcgcca ggctcggggt ataggcggca tcagcaccaa tcagaaccgg tgtttgccgc
     3901 tgactcaatt caaccggtac cgaagccggc ttcacagtgg ccaggtcctg gaaactgaat
     3961 aacccgcgtg cggtatgtac gtctgattcc ccggtttcaa aaatggatag catttcttca
     4021 atcggggtca caccgcccag cggttctttt gaaaaaggaa aactaaacgc aatggcgccc
     4081 accagctttc cattgataaa gaccgggcta ccactcattc cggcaaacac acccgtgcgc
     4141 tcggcgtttg ccccagagag acgaataatg attgaagatt gagtcggcgt tggtagccca
     4201 taaatgacac cgagcacttc gaccccgaac tcttccattt
//
LOCUS       DAOQDO010000227         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14357_length_4237_cov_3.061932, whole genome shotgun sequence.
ACCESSION   DAOQDO010000227 DAOQDO010000000
VERSION     DAOQDO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14357_length_4237_cov_3.061932"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKZ53_04915"
     CDS             complement(<1..723)
                     /locus_tag="PKZ53_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39808.1"
                     /translation="MLSLASDFRLAGRTLLRNPGFAVVVVLTLAMGIGANTAIFSVVN
                     ALLLQPLPYRDAERLAVVWGYNPPQPEDRNVISPFVFRFYRDHMTSFEQLAAFSMPRA
                     TLTGGGAPEEVQLQLTTQNLFGMLGVAPQLGRAFTVEDTRPEAASVVVLSHELWQTRF
                     GGDPNVIGRKVALNSQPTTVIGVMPPYTQFNLLPNSFRFRPAQLWQPVIYSDNFYQTQ
                     LGYLFIIGQLKAGTSLRTAQDEL"
     gene            complement(965..3022)
                     /locus_tag="PKZ53_04920"
     CDS             complement(965..3022)
                     /locus_tag="PKZ53_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ39809.1"
                     /translation="MRPIKHAEKALTKIAGGLFNAIQSVNKYRPAPSFTPKWSDKPIP
                     KSWQKTKPTLGWPRTTDSLCPRCVIEAREQIIKGDKDYTVLINEKVGEIKAQIVKRGN
                     EVWMEKECPIHGKYEDLMAIDAEFLGKIESMFPGRDIDAHNDDKLHNHGTSTIKHGRG
                     SVLTIDLTNRCNMMCDPCFMDANQVGFVHELSLEDIKEILDNAISIKPRRQMSVQFSG
                     GEPTLSPHFFEAIEYSRKLGYNSVQAATNGIEFAKSKEFCRKAAEAGLRYAYLQFDGI
                     GNDANSHRQVGNLFDVKLRAINNMHEAGIEIVLVTTLVNNVNNDQVGPVIEFAMSNPK
                     KIAFVSFQPVSFTGRDEEVTDDRRFMQRYTLSHLAHDVKKQVGITEPTRDWFPISIMG
                     TIADFADLAHGPQAEFGHLSCGCHPNCGVGTALMINKETKEMAPVPQFLNVPQFLKDM
                     TKVTDAGRSKKFSNLMIGLALLKNYDPYGAPTSLSLFDLLKKFDKSLGLTGRNYGRVD
                     GKRTMEDIVKRRQDPWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYNT
                     GVGWRNILEDMHQNAKVAEWYKKYGRHEIFAKNADVPLETTEHHLVINEDHAARVRDR
                     SEAVPMTAAEEKAMMRKLFNEKVLGQKSPEGKLVQLGRNKAKKVQEEPVGAGVPPKPP
                     ETL"
     gene            3580..3801
                     /locus_tag="PKZ53_04925"
     CDS             3580..3801
                     /locus_tag="PKZ53_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39810.1"
                     /translation="MLQPPSPSPPDEEIRDLGFGSIVTRESRQRLLNPDGSFNVERTG
                     LNFWTSLSLYHTLLTLRRLRSWKIQMQSF"
BASE COUNT          995 a         1038 c         1117 g         1087 t
ORIGIN      
        1 cagttcatct tgagctgttc tcagcgacgt accagccttg agctggccaa tgataaacaa
       61 atatcccaac tgggtctggt agaaattgtc actgtaaata accggctgcc agagttgagc
      121 cggacgaaat ctgaaggaat ttggaagcag attgaattga gtatacggcg gcattacccc
      181 gatcacggtt gtgggttgac tgttgagcgc caccttacga ccaatcacat ttgggtcgcc
      241 tccaaatcgg gtctgccata attcgtggct caacaccacg accgacgccg cctccgggcg
      301 agtgtcttca accgtaaagg ctcgtccaag ctgtggagcc acaccaagca taccaaacag
      361 attttgggtt gtgagttgga gttgaacttc ttcaggcgcg ccaccgccag tgagcgtcgc
      421 cctcggcata ctaaaggcag ccagttgttc aaaacttgtc atgtgatcgc ggtagaagcg
      481 aaatacaaat ggcgaaatca catttcgatc ttcaggttgg ggtgggttat atccccaaac
      541 cacagccagt cgctcagcat cgcggtaggg caacggctgg agcaacaacg cattgactac
      601 cgaaaaaata gcggtgttcg caccaattcc catggccagc gtcaagacga caaccaccgc
      661 aaagcctgga tttctgagca gtgtccgccc ggcaaggcga aaatcactgg cgagtgaaag
      721 cataggaaga atgaagaatg aagaatgaag aatgaaaaga tcaccttgtc accttgtcat
      781 tctgtcatat tgccagggtt cggggtttgg ggttttgtgc cccttatgtc ccgtatgtca
      841 tttttgtggt tttcccagag ctgggattac cggactataa ccccatgccc aaaagaaaaa
      901 cagcagtcag ggccagagca ctgactgctg ttttttttaa aggacagatc gggcaagaat
      961 cagcttacag agtttcaggt ggttttggtg gaacaccagc accaaccggt tcttcctgta
     1021 cttttttggc tttattgcga ccaagctgaa ccagcttgcc ttcggggctc ttctgaccga
     1081 gcaccttttc attgaagagt ttgcgcatca tcgctttttc ttcagccgcc gtcattggaa
     1141 cagcttctga acggtcgcgg acgcgggccg cgtgatcttc gttgatgacc aggtgatgct
     1201 cagtggtttc gagcggcaca tcagcgttct tggcaaagat ttcgtgacga ccatattttt
     1261 tgtaccattc ggccaccttg gcgttctggt gcatatcttc gaggatgttg cgccagccga
     1321 ctccggtgtt gtaagcgcag aaggaaattt caccttcctg cgttccataa ggaatgatgc
     1381 acatttctgt ccggcggaaa tcatagttga acagatcctg gaaccacata ccggcaataa
     1441 agaggaagtt ccacggatcc tggcgacgct tgacgatatc ttccatcgta cgcttgccgt
     1501 ccacgcggcc atagttacga ccggtcaacc caagcgattt atcaaatttc ttcagcaagt
     1561 caaacaggct caggctggtc ggggcaccgt atggatcata gttcttgagc agagccagac
     1621 cgatcataag gtttgaaaac ttcttgctac ggccagcatc ggtgaccttg gtcatgtcct
     1681 tgaggaactg tggcacgttc aggaactgtg gcaccggggc catttccttg gtttccttgt
     1741 tgatcatcag cgccgtgcca acaccgcagt ttggatggca gccgcagctc aggtgaccaa
     1801 attcagcctg tggaccatgg gccagatcag caaagtcagc aatggtgccc ataatcgaaa
     1861 tcgggaacca gtcgcgggtg ggttcggtga tgccgacctg tttcttcacg tcgtgagcca
     1921 gatgtgacag cgtgtagcgc tgcataaagc ggcggtcatc ggtcacttct tcgtcacgac
     1981 cggtaaagga cacgggctgg aacgacacga aagcaatttt cttaggattg ctcatcgcaa
     2041 attcaatcac cgggccgacc tgatcattgt tgacgttgtt gacgagggtg gtcacgagca
     2101 cgatttcgat gccggcttcg tgcatgttgt tgatggcacg caatttgaca tcaaacaggt
     2161 tgccaacctg acggtgactg ttggcatcgt tgccgattcc gtcaaactgg aggtaagcat
     2221 agcgcaaacc agcttcggca gccttgcggc aaaattcttt gctcttggca aattcgatgc
     2281 cgttggtggc ggcctgaacg ctgttatagc ccagtttgcg tgagtattcg atggcttcaa
     2341 agaagtgcgg tgagagggtg ggttcgccgc cggagaactg aaccgacatc tggcgacggg
     2401 gcttgatgga aatcgcgttg tcgaggattt ccttgatgtc ttccagcgag agttcatgga
     2461 caaagccaac ctggttggcg tccataaagc acgggtcaca catcatgttg cagcgattgg
     2521 tcaagtcaat cgtcaacacc gagccccgac cgtgcttgat ggtgctggtg ccgtgattgt
     2581 gcagtttgtc gtcgttgtgg gcatcaatgt cacgccctgg gaacatggac tcgattttgc
     2641 ccaaaaactc ggcgtcaatg gccatcaagt cttcatattt gccgtggatt gggcattcct
     2701 tctccatcca aacttcattg ccacgcttca cgatctgagc tttgatttca ccgacttttt
     2761 cattgatcag cacggtgtaa tctttatcgc ccttgatgat ctgctcgcgg gcttcgatca
     2821 cgcatcgggg gcacagcgaa tcagtggttc gtggccaccc aagggttggc ttggttttct
     2881 gccaggattt gggaatcggt ttgtcggacc atttcggggt gaaggaagga gcaggcctgt
     2941 atttgttgac agattgaata gcattgaaga gcccaccggc aatcttggtc aatgccttct
     3001 cggcgtgctt aataggtcgc atgttgttct attctccttg cgtggtggaa gccacgaaat
     3061 gcaaattcgt ccgtcaatca gaaaggattc ggctggatct gcggccagct tccgaaaccg
     3121 ctccgaggcg ctaaatatat gatgccttgt tggttagggt gtagggtagc aacctggaat
     3181 ttaatgactg acgaggcttg ggtaaggcaa tgtgtgttca tctcgtacaa gcacgatgcg
     3241 ctatattcaa caggaatact tactatgtca agctcaagat gccacatgac cgttcgaaaa
     3301 cttcagtttg cgtcaatttt cttcaggttg aagaaaccgg tttaggggtt cagggttcag
     3361 ggttcagggt tttgatttaa acccaggttc ggctccccat tcagctccag gttcaagctg
     3421 gctattggct gattcttgac ggcactataa agatgtatgg gttgagcagg cgggtttgca
     3481 ggtgtgaaac ccggcgacat tgttttcgat tcccggcgtc ttccgccaaa ctcattgtgt
     3541 cactttttga gcccatcaaa tttttatttc atcccattta tgcttcaacc accctcccca
     3601 tcgcctccag atgaagaaat tcgtgatttg ggatttggca gtattgtcac acgtgaaagc
     3661 cgccagcggc tcctcaaccc tgatggaagt tttaatgttg aacgaactgg tctcaacttc
     3721 tggacttcat tgagcctgta tcacacctta ctgacattga gacggctcag gagctggaaa
     3781 attcagatgc agagtttttg attttgcttc agggaatcga agaaaccttc tcacagattg
     3841 tctacacccg ctcatcgtac aaatttgatg aagtcgtgcg ggatgccaga ttcactgata
     3901 tttatagata accggcagaa aaccccgcga ttgatcgcgg ggatgaatgc cgtccgaatt
     3961 tttgcttgct gtgttgaatg gcaccatggt aaatacacag taatgagaca ccgccagtga
     4021 cttcgcgcat cggttagctc tgagcggtag attcaaatcc aaatccactc actcagggaa
     4081 accgatgaga caggcgttca agttccgaat tgatccgagt ccggcagttg aggcaaagct
     4141 caagtggaca ctggatgtct gtcgcgaact gtataatgcc gggcttcagg aacgacgcga
     4201 tgcctaccgg atgtgcgggg tatcgctcac ctatcgc
//
LOCUS       DAOQDO010000228         4234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14371_length_4234_cov_2.767408, whole genome shotgun sequence.
ACCESSION   DAOQDO010000228 DAOQDO010000000
VERSION     DAOQDO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14371_length_4234_cov_2.767408"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKZ53_04930"
     CDS             complement(<1..406)
                     /locus_tag="PKZ53_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ39811.1"
                     /translation="MSNKPDSQPAYFLSLEVENVRCFGPRQTLDVSDGNGRPAQWTVI
                     LGDNGVGKTTLLQCLVGMEPSDSFVFYDEEDLPPDEPARFYEHKISRPRLLFDSFEKY
                     TERFSRLNTFMIEISVSISKGASLNDQKPGESI"
     gene            complement(668..4093)
                     /locus_tag="PKZ53_04935"
     CDS             complement(668..4093)
                     /locus_tag="PKZ53_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ39812.1"
                     /translation="MSSFRVWITLSLLILTVSPSFSPSLYAQTTSGTLIGDVIDPDGK
                     PLANAIVTVVNIDNGTTRATRTLPDGSYRMPFLEPGRYVVKAQREGYQDNQSDTVRIP
                     LNIVTPLNVPPIRLYPVGTALPTTTPQTEAAKINPTDATLRGNIDSQTLLALPIGGIR
                     SFDVMAFLFPGVLPPPATYGANGPGLGPGVGTSGQFSVNGQRGRANNFTVDGSDNNDQ
                     DVGVRRQGFFAPVSQSIESVNEFQISLLLGDAESGRNFGGQVNVVSRSGGNAVHGTIY
                     DFFTTDGLQARDFFDLTGGPSGEENKNRRNQFGFALGGPIVKDRTHVFFSFERQNATR
                     QLEHNFAVPTPAERNSSLIQTTLAQDIFDLFPLPNNSGGPYGASTFTQILPGDADGTL
                     FSARLTHQLKLFGKDHNFTGRYNLTDDETLIPSVGGALRGSLGSRTRTHNISLFLDTT
                     LTATTYNQVRVSFGRTTLGFDEQAGSPFIFQTRPRIDLNGDRQPDGLTGPLGQLAISG
                     LSPIGVDVFTFPQGRTNNTFQYADTLVSTLGKHTLKVGADVRRVQFNSFLDRNYRAQA
                     TFAPGFVVAGASARVGTGTEFVNFGLPSNLVQSLAQTPDSTLGLRQTEMNFFVSDTWR
                     IHPRVTLTMGLRYEYNTVPTDASQRIERTFRLRPEDLPAGDPNLSGFSKVFFDSLASY
                     QQFLDGREKIFEADGNNFAPRVGIAWSPFKSQRLSIRGGYGVYYDPLVGSLTSQSRNV
                     FPNFIPASFGASLIGFNRFNGAFLNPAFFGAGTPPTPLIQPGTTNTVGFQSSLFVLGI
                     GALFSLQAANPGLAFTLPAKELRTPYTHQFGGQVEAVVAKNYVLGVGYVGSRSRQLIR
                     FRTPNLGPNSVYQALFLLPNQPPTLITPRLNRDVRQLGAFTVFDNSAESQFDSLQVRL
                     DRQASDGLQFGMAYTYSHAIDDVSDLFDLTGTFAFAQNDRKLQLDRGSANFDIRHRVV
                     THFVYDLPLARTHRILGGWQVAGIVTLQTGQPFTVNTQLDVNLDGNLTDRLNTLQGLI
                     VSDEGRTRISLAPGTTIDSLRAPFDVDLLLDGSLGRNTFRAQGVATVDLALTKRFAIS
                     ERQNLQFRAEAFNLMNRTHFGIPVRILEAPGFGQSINTSVPARTIQLAVKYVF"
BASE COUNT         1031 a         1058 c         1178 g          967 t
ORIGIN      
        1 gaattgattc acctggtttc tgatcattaa gtgaggctcc ttttgaaatc gagactgaga
       61 tttcgatcat gaaagtgtta agtcgagaga atcgttctgt atatttttca aaggagtcaa
      121 ataaaagtct tggccgggag attttgtgct cataaaaccg cgcaggttca tcaggtggaa
      181 gatcctcttc atcataaaag acaaaagaat cagatggttc cattccaacc agacactgca
      241 acaacgtcgt ttttcccaca ccattatcgc ctaaaatcac ggtccattgg gctggacgac
      301 cattcccatc tgagacatcg agtgtttgtc ggggtccgaa gcagcgtaca ttctcgactt
      361 ccaatgaaag aaaataggct ggttgagaat caggtttgtt gctcataggc tgaaatcctt
      421 ttagtaagct agcattctgc tactgcttcg cggttgaaaa caaatcagct catagcgttt
      481 ggcttcaagg aaaatttaaa ccagccgctt gttggtatgt caccttttca aagcttaacc
      541 tttgatgtaa cgggagccga ggcttgagtc accttggctg ttggaatgat atctgcactg
      601 agcaaaaatt cttatgcccg aagtcttcag cccattttct tcagccccaa gccctcagcc
      661 ccagtatcta aaatacatac ttcaccgcca gttggattgt ccgggcggga acagatgtgt
      721 tgatggattg gccaaatcca ggggcttcta aaattcgcac cgggatgccg aaatgggtgc
      781 gattcatcag gttaaaggct tcggcgcgaa attgcaggtt ctggcgttcg gaaatggcaa
      841 aacgtttggt caacgccaga tcaaccgttg ctacgccttg ggcccggaag gtattgcgac
      901 caaggctgcc atccagcaac aaatccacgt caaacggcgc ccgtagcgaa tcaatcgtgg
      961 tccccggagc gagggaaatc cgcgttcggc cttcatccga tacaatcaat ccctgcaagg
     1021 tgttgagtcg gtcagtcagg ttgccgtcca ggttgacatc cagttgggtg ttgacggtga
     1081 agggctgccc ggtttgcaat gtgacgattc cggccacctg ccagccgccc aggatgcggt
     1141 gcgttcgggc cagcggaagg tcatacacaa aatgcgtgac gacccgatga cgaatatcaa
     1201 aattggcgct ccctcgatca agctgcagtt tccggtcatt ttgggcaaaa gcaaacgtgc
     1261 cggtcaggtc aaacaggtct gatacatcat caatcgcgtg tgagtaggta taggccatcc
     1321 caaattgcaa cccatcgctg gcctggcgat caagccgcac ctggagcgaa tcaaactggc
     1381 tttcggctga attatcaaac actgtgaagg cgccaagctg gcgaacgtcc cgattgagtc
     1441 gcggcgtgat cagtgtcggc ggctggttgg gcaacaaaaa cagcgcctgg tagaccgaat
     1501 ttggccccag gttgggcgtt cggaagcgga tcaactgccg gcttcgcgaa ccgacatatc
     1561 cgacgccaag cacgtaattt ttggcgacca cggcttcaac ctgtccacca aattgatggg
     1621 tgtagggcgt tcgtaactcc tttgccggca gtgtaaacgc cagtcctgga ttggcagctt
     1681 gaagggaaaa taaggcaccg attccgagca caaacaggct ggattgaaaa ccgactgtgt
     1741 tggtcgttcc aggctgaatc aatggggttg gtggtgttcc ggcgccaaaa aacgccgggt
     1801 tgagaaatgc gccgttaaac cgattgaacc caatcaatga cgctccgaag ctggctggaa
     1861 tgaaatttgg gaagacattg cgcgactggc tggtcaggct cccaaccagc gggtcgtaat
     1921 aaaccccgta tccaccacga atcgaaagcc gctggctttt aaatggcgac caggcaatgc
     1981 cgacgcgagg tgcgaagtta ttgccgtccg cttcaaagat tttctcccgt ccatccaaaa
     2041 actgctggta agacgccagc gagtcaaaaa acactttgga aaatccgctc aagttcggat
     2101 cgcccgccgg aagatcttcc gggcgcagcc ggaaggtacg ttcaatgcgc tggctggcat
     2161 cggttggaac ggtgttgtat tcgtaccgca ggcccatcgt gagcgtgact cggggatgga
     2221 tgcgccacgt gtctgataca aaaaagttca tttctgtctg gcgtaaaccc aaggttgagt
     2281 caggtgtttg ggccagcgat tgcaccagat tggagggcaa cccgaaattg acaaattcgg
     2341 tgccagtacc aactctggcc gacgccccag ccaccacaaa ccctggcgca aacgtggctt
     2401 gtgcccggta gttgcgatcc aggaagctat tgaactggac gcgacggaca tctgctccaa
     2461 ctttgagcgt gtgtttgccg agtgtgctca ccagcgtgtc agcatattga aacgtgttgt
     2521 tggtccgtcc ctgtgggaag gtaaacacgt ccacgccgat agggctgaga cccgaaatgg
     2581 caagctgccc aagtggtcca gtcaatccat caggttgacg gtcgccattg agatcaatcc
     2641 ggggacgggt ttgaaaaata aatggactgc ccgcctgttc gtcaaagcca agtgtcgttc
     2701 ggccaaatga aacgcgcacc tgattgtagg tggtggccgt gagcgtcgtg tccagaaaga
     2761 gcgaaatgtt gtgcgttcgg gttcgcgacc caagcgaccc gcgcaaggcg ccgcccacgg
     2821 aaggaatcag cgtttcgtca tctgtcaaat tatagcgtcc agtgaagtta tggtctttcc
     2881 caaacaactt gagttggtgc gtgagccggg cagagaaaag cgttccatcg gcatctcccg
     2941 gcaaaatttg agtaaaggtg ctggccccat aggggccacc gctgttgttg ggcaacggaa
     3001 acagatcgaa gatatcctgg gccagtgtgg tttgaatgag gctggaattg cgttctgctg
     3061 gggttggaac ggcgaagttg tgctcaagtt gacgggtggc gttctggcgc tcaaacgaga
     3121 aaaagacgtg tgtccggtct ttcacaatcg ggcccccgag cgcaaagcca aactggttgc
     3181 ggcgattctt gttttcttcg ccagatgggc cgccagtcag gtcaaagaaa tcacgtgcct
     3241 gcaggccgtc cgtcgtgaag aaatcataaa tcgttccgtg gacagcattt ccgcctgatc
     3301 tggacaccac attgacctgt ccgccaaagt tacgtcctga ttcggcatcg cccagcagca
     3361 ggctgatttg aaattcgttg acgctttcaa tggattgcga gaccggggca aaaaagcctt
     3421 gccggcgaac acccacatcc tggtcattgt tgtccgaacc atccaccgtg aaattattgg
     3481 ctctcccacg ttgaccattg actgaaaatt ggccggaagt tccaacacca gggccaaggc
     3541 cagggccgtt agcaccatac gtcgctggcg gaggcaggac gcctgggaag agaaatgcca
     3601 tcacgtcaaa actgcgaatt cccccaattg gaagtgccag cagagtttgc gaatcaatat
     3661 tgccgcgcag ggtggcatcg gtcggattga ttttggccgc ttcggtttgt ggtgtggtgg
     3721 tcggcagtgc ggtgcctacc ggatataatc gaattggcgg cacgttgagt ggggtcacaa
     3781 tgttgagtgg aatccgaacc gtatcgctct ggttatcctg atatccttcg cgctgggctt
     3841 tgaccacata gcggcccggc tccaaaaacg gcatccgata tgacccatcc gggagcgttc
     3901 gcgtcgcacg cgtggtgccg ttatcaatgt tgaccacggt tacaatcgca tttgccagtg
     3961 gcttgccatc tggatcaatc acatctccga ttaacgttcc agaggttgtt tgggcataaa
     4021 gtgatggtga aaacgagggt gagactgtca aaatgagcag gctcaacgtg atccagactc
     4081 taaaagaaga caaagcgggt ttgagaaaac cggtcatgcg gcctccgcga cctgaaaaga
     4141 tgggtggtga gtggtacttg gtgagtggtg cttggtacct ggtgcttggt gcttggtgct
     4201 ttggaaccaa taccttcaaa gaacaaagaa cgaa
//
LOCUS       DAOQDO010000229         4228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14412_length_4228_cov_3.121735, whole genome shotgun sequence.
ACCESSION   DAOQDO010000229 DAOQDO010000000
VERSION     DAOQDO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14412_length_4228_cov_3.121735"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..751
                     /gene="thiE"
                     /locus_tag="PKZ53_04940"
     CDS             77..751
                     /gene="thiE"
                     /locus_tag="PKZ53_04940"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNJ39813.1"
                     /translation="MTIPSIEGLERVQPMALPPLYAITDQPLTGLSHLELVKHLIAGG
                     ATLIQLRDKVTDGRTFYELAHQVMEYARPHGVKIIINDRVDIALAVNADGVHVGQDDL
                     PPAAARQLLGPSKLIGYSTHSLDQALEADQFPIDYIAVGPVFQTQTKENPSPVVGLEL
                     VRRVATQVSKPVVAIGGIPPERISEVLQAGATSVALISGLLLPVDSLAGRTRSLLSAL
                     CHQGKI"
     gene            936..2633
                     /locus_tag="PKZ53_04945"
     CDS             936..2633
                     /locus_tag="PKZ53_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNJ39814.1"
                     /translation="MQQFALWPFSTRPAGNFRGRLKQGLLWGLLCIFLFGQTPIVWAT
                     SSGLPARAENNEINPEKMLKAARKLVRQGDWQTAETNLRQILTADPKYYPARLELAYL
                     YVKQRRLSEAYDEISFTTTSEPPLARAHSIKGIALLRSGYFGLAYDELKLALGIDQQD
                     DLALAAIAEVAYYENQLEQAERLINNALSRNHNEPDYYLLQARIASRMERFRVAADAL
                     KDFLRVTPKTDIERRDRIEGVIRFYSYLGDSHTNVVEDPRSATIPLTIRNRRPHVKVK
                     INGKGPLNFVIDTGAGISVVSLTTAEKLGIKPVAQGGQARAVGGQGAFPIVYGFLKSL
                     EIGDIKITSVPVYLREVQGARTSAPNEGTDGFLGLSVLNEFLVTLDYQSKELKMEPNA
                     RTGQPIVTQAIDPQSSAVPFRFTESGLISIETTLDGAEKMHFIFDSGASISVISDRAV
                     KRLHWEDKILDAEKIHIVGAAGVTQNVEMLQAKTVQVADLVKQDVRLPVLDLVRLNES
                     AGFEQHGILGGDFLYHCRVQINFATQNLIFTPFNDAVRRVNLQTTKELDKHETEPVP"
     gene            2611..3075
                     /locus_tag="PKZ53_04950"
     CDS             2611..3075
                     /locus_tag="PKZ53_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNJ39815.1"
                     /translation="MKLSLYLETSVIGAYLDNDDPFRRDLTIRWWELEMHTFRPLTSL
                     LVQRELERLPEPYRASYLNLIREIPQVEISDEAAILADGYLSRGIFQRKYMADALHVA
                     LASIHKIDFLVTWNFGHIANVQRQTRVRLFNTSSGFFSPMIVTPEFLVSSNV"
     gene            3098..3319
                     /locus_tag="PKZ53_04955"
     CDS             3098..3319
                     /locus_tag="PKZ53_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39816.1"
                     /translation="MYRKPRFLQELHAIREEMARECDYDVDLLAEMVRSGHRPPHGPQ
                     QIIRGRRVFSPPQEDQVKRQSPVNETDFQ"
     gene            3316..>4228
                     /locus_tag="PKZ53_04960"
     CDS             3316..>4228
                     /locus_tag="PKZ53_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ39817.1"
                     /translation="MTRFTALLMISAFLGTGTWVGLHTPVWARPKSLMAQKEKTSRLA
                     KKTPPPPQKNNPQPASAGKSGIPVPSVLPVNIADVVAQVDQSVMNIQATAEDGSISIG
                     SGFCLDSKGLIVTNFHVIRGSLKLDGPITVVTPDARTLTASLRGYDEATDLALLEVDF
                     GEKPIPALPFGDSDTLRVGDWVIAIGSPFELDHTVTLGIISGKGRTGLDGEYDDFLQT
                     DAAINFGNSGGPLVNLKGEVVGINTLVLVRAQGLGFAIPINILREITPQLRENGFVRR
                     SSLGVETRDISAIVRRTLGLGTQTRGVL"
BASE COUNT         1017 a         1050 c         1104 g         1057 t
ORIGIN      
        1 gagaagggta taaattcaat gaagccctgc tccaggccct ggttgaaggg tccagcgaga
       61 agaaaggctg acgccgatga caataccatc aattgagggg ctggaaaggg ttcaaccgat
      121 ggcacttccg ccactctatg ccattacaga tcaacctctg accgggctat cacacctcga
      181 actggtcaaa cacctgatcg caggtggagc cacgctgatc cagttgcgcg ataaagtcac
      241 ggatggtcga acattctatg agttggccca ccaggtcatg gagtatgccc gcccccacgg
      301 agtcaaaatc attatcaatg atcgggtgga tattgcactg gccgtcaatg cggatggggt
      361 tcatgttggt caggacgatt tgcctccagc cgcagcccgc cagttgttag ggccatcaaa
      421 actgattggc tactcaaccc attcgctgga tcaggccctg gaagctgatc aatttccgat
      481 tgactatatt gcggtcggac ctgtttttca gacccaaacc aaagaaaatc cttccccggt
      541 tgtcgggctg gagctggttc gccgggtggc gacccaggtt tccaaaccgg tcgtggcgat
      601 tggcggtatc ccaccagagc ggatttctga ggtccttcag gcgggggcaa ccagcgtcgc
      661 cctgatttca ggtctcctgc tgcctgttga ttcgctggcg ggtcgtaccc ggtcccttct
      721 ttcagcactc tgtcaccagg gtaaaatata gaaaaaacac cacttaattc ttgttttgac
      781 agacttgctg gtaagcccta caatttattt ttcccacaac gtcgtaagca ccaatctcgg
      841 gatgctggct tcccaatcct tccaggccgc cgtgtcactt gtctgtgatt cggttttatc
      901 gtgcgggcga caatcattaa ggaggtatca ggcatatgca acaattcgct ttgtggccct
      961 tctccacccg gcctgcaggt aactttaggg gaagactcaa acagggacta ctgtggggct
     1021 tgctgtgtat tttcttgttt gggcaaaccc caattgtttg ggcaacatca tctggcttgc
     1081 cagccagggc agaaaacaac gagattaatc ctgagaaaat gctcaaggcg gcgcgcaagc
     1141 tggtgcgcca gggcgactgg caaaccgcag agaccaatct tcggcagatt ttaacggctg
     1201 acccgaagta ctaccccgcc cggttagaac tggcctatct ctatgtcaaa caacgccgct
     1261 tgagcgaagc ctatgatgaa atttcattca ccacgacatc cgagccaccg ctggccagag
     1321 cccattcgat taaagggatt gctcttttgc gctcaggcta tttcgggctg gcgtatgatg
     1381 aattgaaact ggcattgggt attgaccagc aggacgatct ggcactggca gctattgctg
     1441 aagttgccta ttatgaaaat caactggagc aggcggaacg gttgattaat aacgctttat
     1501 cacgtaatca caatgagcct gattactacc tgttacaagc acggattgcc tcacggatgg
     1561 agcggtttcg cgtggccgcc gatgcgctca aagattttct tcgagtgacg ccgaaaactg
     1621 acattgagcg ccgtgatcga attgaaggcg tcattcggtt ttattcatac ctgggtgatt
     1681 ctcacaccaa tgtggttgaa gaccctcgaa gcgccactat cccgctgaca attcgcaatc
     1741 gccggcctca tgtcaaagtc aaaatcaatg gcaaagggcc gttaaatttt gtgattgaca
     1801 ctggtgccgg catttcagtg gtttcactga ccacggctga aaaattagga attaagccgg
     1861 tggctcaagg tggtcaagcc cgagcggttg gtgggcaggg agcatttcca attgtctatg
     1921 gttttttgaa aagccttgaa attggcgata ttaaaatcac gtcagtgccg gtgtatttac
     1981 gggaagtcca gggcgcccga accagtgctc cgaatgaggg aactgatggc tttttggggc
     2041 tatcggtctt gaatgaattt ctggtgacgc ttgattatca atcaaaagag ttgaagatgg
     2101 agccgaatgc cagaaccgga caaccgattg tgacccaggc aattgacccc caatcaagcg
     2161 ccgttccatt ccggtttact gaaagcggtc tgattagcat tgaaaccaca ctcgatggtg
     2221 cagagaaaat gcattttatt tttgactctg gcgccagtat cagtgtgatt tctgaccgcg
     2281 ccgtcaaacg acttcattgg gaagacaaaa tcctggacgc ggaaaaaatt catattgtgg
     2341 gggcagccgg ggtaacccaa aacgtggaaa tgcttcaggc aaagacggtt caggtcgctg
     2401 atttggtgaa acaggatgtt cgacttccag tgctcgattt ggtgcggtta aatgagtcag
     2461 ccgggtttga gcaacatggg attttaggcg gagatttcct gtatcattgt cgggtacaaa
     2521 tcaattttgc cacccagaac ctgattttta cgccttttaa tgatgccgtg cggcgtgtca
     2581 atctgcagac aaccaaagaa ctggataagc atgaaactga gcctgtacct tgaaacttcg
     2641 gtaattgggg cctacctgga taatgatgac ccgtttcgcc gggatttaac catccgctgg
     2701 tgggaactcg aaatgcatac ctttcgcccc ctgacatcac ttctggtaca gcgtgagtta
     2761 gaacggcttc ctgagccgta ccgtgcctca tatttaaatt tgattcgaga gatcccgcag
     2821 gttgaaattt ccgatgaagc cgccattctg gctgacggat atttaagccg tgggatcttt
     2881 cagcgtaaat atatggccga tgcccttcac gtggcgctgg cctccattca taaaattgac
     2941 tttctggtga cctggaattt tgggcatatc gctaatgttc agcggcaaac cagagtacgt
     3001 ctctttaaca catcaagtgg ttttttctca ccgatgatcg taaccccgga atttctggtg
     3061 tcgagtaacg tgtgaggagg cttgaggact ccgggatatg tatcgtaaac cacgcttttt
     3121 acaggaatta cacgcgatcc gtgaagagat ggcacgtgaa tgtgactatg atgtggattt
     3181 gctggctgaa atggttcgct ctggccatcg cccaccacac ggtcctcagc aaattattcg
     3241 cggacgccgg gttttttcac ctccccagga agaccaggta aaacgtcaat ctcctgtaaa
     3301 cgaaacggat ttccaatgac acgattcact gcgctcttga tgatttcagc gttcctgggt
     3361 actggaacct gggttggtct tcacacgccc gtttgggcca ggcccaaaag cttgatggct
     3421 caaaaggaaa aaacctctcg cctcgccaaa aaaacaccgc ctccgccaca gaaaaacaat
     3481 ccacaaccag cgtcagctgg caaaagcggt atccctgtac catcagttct cccagtaaat
     3541 atcgctgatg ttgtagccca ggtggatcaa tcagtgatga acattcaagc cacggctgag
     3601 gacggcagta tttcgattgg tagcggcttt tgtttggatt cgaaagggct cattgtcacc
     3661 aactttcacg tcatccgggg atcgctcaag cttgatggcc caatcacggt ggttacccca
     3721 gatgcccgca cattgacggc cagcctgcgc ggatatgatg aagccacgga tctggccctg
     3781 cttgaagttg attttgggga aaaaccgatt ccagccctgc cgtttggtga cagcgatacc
     3841 ctccgggttg gggactgggt cattgccatt ggcagccctt ttgagctgga ccataccgta
     3901 acgcttggca tcatcagcgg gaaaggccga accgggctgg atggtgagta tgacgacttt
     3961 ttgcagacag atgcggcaat caattttggc aattccggtg gcccgctggt gaatttgaaa
     4021 ggtgaagtcg ttgggatcaa tacgctggtg ctggttcgcg ctcagggtct tggatttgcg
     4081 attcccatca atattttacg ggaaatcaca ccccaacttc gtgaaaatgg atttgtccgc
     4141 cgtagttcgc tcggggtgga aacccgtgat atttcagcca ttgtccgccg cacgctcggg
     4201 cttggaaccc agactcgtgg tgtattgg
//
LOCUS       DAOQDO010000230         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14418_length_4227_cov_3.007191, whole genome shotgun sequence.
ACCESSION   DAOQDO010000230 DAOQDO010000000
VERSION     DAOQDO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14418_length_4227_cov_3.007191"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PKZ53_04965"
     CDS             complement(<1..141)
                     /locus_tag="PKZ53_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ39818.1"
                     /translation="MSVVFPRAERLIAMQPSSTMAATQAAARLRAAGHDVIDLGAGEP
                     DFT"
     gene            complement(369..857)
                     /gene="coaD"
                     /locus_tag="PKZ53_04970"
     CDS             complement(369..857)
                     /gene="coaD"
                     /locus_tag="PKZ53_04970"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNJ39819.1"
                     /translation="MTRAIYPGSFDPLTNGHLDIIERSSKLFDEIIVAILVNPEKRPM
                     FSTEERLEMIREVVAPLAKVKVDTFNGLLVTYAQEQQADGIVRGIRAISDYEYELQMA
                     LMNRRMQPNIETVFMMSADTYSYVSSRLVKEIFLLGREVTGLVPPLIADRMRQKLSDG
                     AR"
     gene            1223..4024
                     /gene="ileS"
                     /locus_tag="PKZ53_04975"
     CDS             1223..4024
                     /gene="ileS"
                     /locus_tag="PKZ53_04975"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNJ39820.1"
                     /translation="MSTETTLDLKKTVNLPSKQLPMKGNLTQAEPARLKRWNEKDVYG
                     EMRKARAGRPMFLFHDGPPYANGKIHLGTAFNKILKDFVLKSRVMMGFDVPYIPGYDC
                     HGLPIEKKVEEELGKKRFEMSAMQIRRAARKFAEKHIKVMNQDFQRLGIFGDWENAYE
                     TMSFGYEAAILRTLGDFMKLGSVYRGLRPVHWSIGAQSALAEAELEYKDVTDPSVYVA
                     FPLKSDPAELDPALAGKSVSVVIWTTTPWTLPANLGICFGPTFDYVVVEVSGENYLVA
                     EELLKAVSEKCDWTEPQVIARFKGQVMDRKAAWHPWIERESLCMVGDHVTLDAGTGCV
                     HTAPGHGAEDFYIGKEYGLDAYCPVDGRGHFTEEVEHFAGMQVFEANPKIVAFLKEKG
                     RLLKVEDYFHSYPHCWRTNTPTIFRATPQWFISMDSADLRQKALDEINKVSWLPTWGR
                     DRMSKMFVDRADWCISRQRAWGVPITALKCSDCATEYVSADLMYRVAEFFEKEGADAW
                     YLREFTDFVEPGFKCGHCGSEKFEKEMDILDVWLDSGTSWQVMERAGLCTPEEPASDV
                     YLEGSDQYRGWFNSSLIVSLGLRNHAPYKQIITHGYVVDGKGQKFSKKLGNAIEPQEL
                     INKGGVDIIRLWVASLDYTEDVRISDEIMARISDAYRKIRNTACYLVNNLYDFDPKQD
                     AVPVSELMEIDRWALVKMNEVVKTVREAYDRYDFQAAYFALYGFCATELSNIYFDVLK
                     DRLYTCAPKSFERRSAQTGLYEIVHRMARLLAPFIVFTADEIWEKLPHPEGADVSIHV
                     TEFPDYDKNLADPALLARWENLLEVRSTVLKALETERVSGHIGSSLEAQVTLAVSGEV
                     AEILNSYGEDALAFFFIVSGVKLTQAEGTDVQVTVSLANGIKCERCWHYETTVGQDER
                     FPTLCHRCATNVEAGWLKN"
BASE COUNT         1058 a          972 c         1167 g         1030 t
ORIGIN      
        1 ggtgaaatcg ggttcacccg cgccgaggtc aatcacatca tggccagccg cccgcaaacg
       61 agccgccgcc tgagtggcag ccatggtgga tgagggttgc atggcgatga gccgttcagc
      121 ccgaggaaaa acgactgaca tagttaatac cttttaagaa agagggttcc gggtttggaa
      181 accagggttc agggttcagg gttcagggtt tttggaaggg atgagggatg aaggatgagg
      241 gatgaaaaaa tcaccttgtc accttgtcat tcggtcatct ggtcagggtt tcggggttca
      301 gggttcagga ttcaaggttt agagttttcg aagaatcctc cgctgatacg ggtgattctg
      361 aatactcgct atctcgctcc gtcactcagt ttttgtcgca ttcggtcggc aatcagcggt
      421 ggaaccagtc cagtgacttc gcgtcccagc aggaagattt ccttgaccag gcgggagctg
      481 acatagctgt acgtatcagc cgacatcata aacacggtct cgatattggg ctgcatccgg
      541 cggttcatca gggccatctg cagttcatat tcatagtcgg aaatggctcg aatgcctcgc
      601 acgatgccat cggcctgctg ttcctgagca taggtcacaa gcaatccatt aaaggtgtct
      661 actttgactt tggccagcgg ggcgacgact tcgcgaatca tttccagtcg ctcttcagtg
      721 gaaaacatcg gacgtttttc cgggttgacc agaatagcca caataatttc atcaaacagc
      781 ttgctactgc gctcgataat gtcgagatgc ccattggtta acgggtcaaa ggagccaggg
      841 tagatggcac gtgtcacacg aacctctcgg ggatgttggg ttgattcagg tgttttccgg
      901 ggaagaagcg cgatgctaaa tcagttggtt acatgatgta aactgtttgg gtgagaatga
      961 agaatgaaga atgaagaatg aagaatgaag aaatctggtt aagaatcaga aaagtctttg
     1021 tctgtgagta ctttggtgtt ctggttgctt catcctggag cctttttttc tctaagccgg
     1081 ggcggttgcc aggcggaagg ccgcctttca atttgcttga cagcggctgt ttcagcataa
     1141 tgaacaagtg ttcaatttct cgctggctgc gaaattccta cgcaacatca cgcaaactat
     1201 tgaggagtaa gaagaaaagg aaatgtctac ggaaaccacg ctcgatttga aaaaaacggt
     1261 caatttgcct tccaagcaac ttccgatgaa aggcaacctg acgcaagctg aaccagcccg
     1321 tctcaaacgc tggaacgaaa aggacgtcta tggcgaaatg cgcaaggcac gtgccgggcg
     1381 tccaatgttt ttgttccacg atggtccacc ctatgccaat gggaaaatcc acctgggaac
     1441 ggccttcaat aagattttaa aggatttcgt cctcaaatcg cgtgtgatga tggggtttga
     1501 tgtgccctat attccgggct atgactgcca cgggctgccg attgaaaaga aagttgaaga
     1561 agagcttggc aaaaaacgct ttgaaatgtc ggccatgcaa atccgccggg cagcacgcaa
     1621 gtttgctgaa aaacacatca aggtgatgaa ccaggatttt cagcggctcg ggatttttgg
     1681 cgactgggaa aatgcctacg aaacgatgag ctttggctac gaagccgcga tcttgcggac
     1741 gctgggcgat tttatgaagc tgggcagcgt ttatcggggg ttgcgcccgg ttcactggtc
     1801 catcggtgct cagtcagccc tggctgaagc tgaactcgaa tacaaagatg taacggatcc
     1861 gtcggtgtat gtggcgtttc cgctcaaatc agacccggct gaacttgacc cggcgctggc
     1921 tggcaaatcg gtgagcgtcg tgatctggac caccacgccc tggacactcc cggccaacct
     1981 gggtatctgt tttggaccca cgtttgacta tgtcgtggtg gaggtcagcg gcgaaaacta
     2041 tctggtggct gaagaattgc tcaaagccgt cagtgagaaa tgtgactgga ctgagccgca
     2101 ggtaattgcc cgcttcaaag gtcaggtgat ggatcgcaag gccgcctggc atccgtggat
     2161 tgaacgggaa tcgctgtgta tggtgggcga ccacgtaacg ctcgatgccg gaacggggtg
     2221 tgtccacacg gcacctggac acggtgctga agacttttac atcggcaagg agtatggcct
     2281 ggatgcttat tgcccggttg atggccgagg tcattttacc gaagaagttg agcattttgc
     2341 cgggatgcag gtctttgaag ccaatccgaa aattgtcgct tttctgaaag agaagggacg
     2401 gttgctgaag gttgaggact acttccacag ctatccgcat tgttggcgga ccaacacccc
     2461 aaccatcttc cgggccacgc cgcaatggtt tatttcgatg gattctgccg atttgcgcca
     2521 gaaggcactc gatgagatca acaaagtatc gtggctgccg acctggggac gcgaccggat
     2581 gagcaaaatg tttgttgacc gcgctgactg gtgtatttca cgccagcggg cgtggggtgt
     2641 gccgatcaca gcactcaagt gcagcgattg tgccaccgaa tatgtcagcg ctgatttgat
     2701 gtaccgggtg gctgaatttt tcgagaaaga aggggctgat gcctggtatc tccgtgaatt
     2761 cacagacttt gtagaacctg gattcaaatg cggtcactgt ggttctgaaa agtttgagaa
     2821 ggaaatggat attctcgacg tctggcttga ttccgggaca agctggcagg tgatggagcg
     2881 agccgggttg tgcacacctg aagaaccagc gtcggatgtc tatctcgaag gctcagatca
     2941 gtatcgcggc tggtttaatt cctcgttgat tgtgagttta ggattgcgta accatgctcc
     3001 gtacaagcaa atcatcaccc acgggtacgt cgtggatggc aaagggcaaa agttctcgaa
     3061 aaagcttgga aatgccatcg aaccgcaaga actgatcaat aaaggcggag ttgatatcat
     3121 ccgcctctgg gtcgccagcc tggattacac cgaggatgtc cgaatttcag atgaaatcat
     3181 ggcgcgcatc agcgatgcct atcgcaagat ccgcaatacg gcctgctacc tggtcaataa
     3241 cctgtatgac tttgatccca aacaggatgc cgttccggtg tcagagttga tggaaattga
     3301 ccgctgggcg ctggtcaaaa tgaatgaagt cgtcaaaacc gtgcgcgaag cctatgaccg
     3361 ctatgatttt caagcggcgt actttgcact gtatgggttt tgtgcgacgg aattgtcaaa
     3421 catctacttt gatgtgctca aagatcggct ctatacctgc gcaccaaagt cctttgaacg
     3481 acgctcagct cagaccggac tctatgaaat cgtgcatcgg atggcccggt tgctggcccc
     3541 gttcattgtg tttacggcag atgaaatctg ggaaaaattg ccgcatccgg aaggtgctga
     3601 tgtctcaatt catgtcactg aattcccaga ttatgacaaa aatttggcgg atccggcatt
     3661 actggcacgc tgggagaatc tgctggaggt ccgatcaacc gttctcaaag cgcttgaaac
     3721 cgaacgtgtt tccggacaca ttggctcatc acttgaagct caggtgacgc tggcggtcag
     3781 tggtgaagtc gcagaaatcc tgaactcata tggcgaagat gccctggcgt ttttctttat
     3841 tgtgtcaggg gtcaagttga cccaagccga aggaactgac gtgcaggtaa ctgtttcgtt
     3901 ggcaaatgga atcaagtgcg aacggtgctg gcactatgaa acgaccgtgg gccaggatga
     3961 acgattccca accttgtgcc atcgttgcgc taccaacgtc gaagccggct ggctcaagaa
     4021 ttgaaaatga agaattgaga atgaagaatg aagaaatttt ggtacatcgt acctggtgct
     4081 tagtgcttag tacttaggga tcaatacttt caaagaaaaa ccaggaacca agaaccaaga
     4141 accaagaacc aagaaccaag aaccaagaac cagttcttca ttcttcattc ttcattcttc
     4201 attcttcatt cttcattctt cattctt
//
LOCUS       DAOQDO010000231         4222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14436_length_4222_cov_3.514519, whole genome shotgun sequence.
ACCESSION   DAOQDO010000231 DAOQDO010000000
VERSION     DAOQDO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14436_length_4222_cov_3.514519"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            655..>4222
                     /locus_tag="PKZ53_04980"
     CDS             655..>4222
                     /locus_tag="PKZ53_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39821.1"
                     /translation="MQWLARLSGLFLVCSLLAGQGFAAPQPVPSSSYPVATQAVIDLE
                     DAGNYVFRSFTDRDGLPQNTVTSLAFDTKGYLWVGTQDGAAFYNDRTWTIVNLPTRNQ
                     TNYIYTILCAKDGALWFGTNGGGLLCLKDGQWKQFEAQEGKLPGNEIRCLLESTTPDG
                     KQILWIGTSNRGLAKLEGDIWTVYTTENSGLPHNGIISLYETSDPDGKRSVWVGTPGG
                     LGCLQNNQWTNFTTKNSGLPKDGIFCVTQTITTDGKRTLWAGTAGGGLARLDQNQWTV
                     YDTSNSGLPGNIVRTLLPIQEPDGSTTLWVGTYGGGIARFARGQWKVLNHKISGLLND
                     GIWTIGRSPGPLGATTLWIGMLSGLSRLKLDQWKVYQPTAEQTQHVPVMSLLESVSGT
                     QRALWIGSAGGGLSKLENGQWVTYDTQNSGLPNNRVLDLLETRGTDNSTTLWIATNGG
                     GLATLKNGQWTVYDTKNSGIPHDLVWSLQPTRTVEGKPAVWVATYGGGIALFDETGKW
                     TVLDTKNSKIPNNLVMCLLLSRAADGSETLWVGTDGAGLGCFRNGQWTTFNTSNSRLP
                     NNVVMSLEETEGPNGSRHLWVGTAGGGAVRFDPVRPAEPWLHLTDTSNPPLPNTTVNQ
                     ICLDAQKRVYLFTNKGISRLTPRSPTTENASAFDLFTYTTEDGLPSMECNLGASLVDS
                     RGNIWAGTVEGTVMLSPAKSQEDRVPKPLHLERILINGQELNQTQIRQFEPPTLGYDE
                     NNLVFEYSLLSYIHEDATTYQVQLVGFDDAPSAWRKDFKKEYTRLPAGSYEFQVWGRD
                     YAGNITEGPHLRFRIKPAPWLTWWAFLIYVGIGASVVYGMYEWRMQQLFRRQQERLVH
                     LRQLQDQRVSTLRQLLESIRVINSELDLSTVLKKIAEEGAQLVDGEPGGIGLIEKETV
                     VFRPIWINKEWQEEPLIVQFGEGIAGKAAQKGQTVIVNDTSTFPEMDVRERLARDGIF
                     GLMNVPIYSRTGTIVGILDVRRPKDRAPFSESDRRLVESFAHQAAIVIEKAALYSELE
                     EKNLMIVESMRELQRLYKQEQEVSRSLQELDQMKNNFLIVTSHELRTPLTVISGYTEI
                     LLDEYLGQVNDKQRQSLKTCREMTSRMADSLNNILEMLRISEGYITLNQSEHDLAQMI
                     RNIISELQGFAHLRNQRLLAEVPATLVLTIDAEKIELLLLNLI"
BASE COUNT         1063 a         1078 c         1140 g          941 t
ORIGIN      
        1 ctggccgcaa acacgtggct ggcccccggg ccacccaaga aacgccatca ggaggcccag
       61 ccagaaaatg gagcgtctcg cacagaacag cgtcaggggt tgccgaccag tctgagccga
      121 cagtgctttc cgcatccggc atagtcccag ccagattgtg gatggttgaa ggttcacata
      181 aacctcacag gatgaagagc ttggacgtga cggatgctgg aaccagagcc ttgggcttgg
      241 gaaaatacaa attcgtcatc gttgaggccg ttgagaattc gaggagctga ggtgaaaagg
      301 gtgtcaatgt cagggtctac cagcttggaa tgaggtcata tcagaatacc aggagccaac
      361 tcattgaaga aattgtattt ccctgagccc aaagcccaaa gtcccaactc caaaatggtt
      421 ttacaggatg aaaggtggta ccagacagaa caaattgtag cagaaaaccg ggcagtttcg
      481 acgaagaatt ctgttgacgg cagatccagg ctccgttcgg acctttcaaa atgcttgcca
      541 gaacctcttt ttctggtgca tggtttggca tcacttcacc tggcgacccc aaacaaaaga
      601 ttcctcccaa aagagttact ggcgagatgg gaggtccaaa aacgaggcag gcccatgcag
      661 tggctggcac gattgtctgg actttttcta gtttgcagcc ttctggctgg tcagggattt
      721 gctgcacctc aaccagtgcc ctccagttca taccctgtgg ccacccaggc cgtcattgat
      781 ttagaagatg ctggcaatta tgtctttcgg tcatttaccg accgtgatgg acttccccaa
      841 aatacagtca cctcactcgc ttttgacacc aaagggtatc tctgggtggg aacgcaggat
      901 ggggcagcct tttacaacga ccgaacctgg accatcgtca acctgccgac acgaaaccag
      961 accaattata tctacacgat tttatgtgcc aaagacggcg ccttgtggtt tggaaccaat
     1021 ggaggaggac tgctctgcct caaagatggc cagtggaaac aatttgaagc ccaggagggg
     1081 aaactccccg gtaacgaaat tcgatgcctc ctcgaatcaa ccacacctga tggcaagcag
     1141 attctgtgga ttggcacctc aaatcgagga ctggccaaac ttgaaggtga catctggacg
     1201 gtttacacca ctgagaactc agggttgccg cataatggca tcatcagtct gtatgaaacc
     1261 tctgatccgg atgggaagcg ctcggtgtgg gttggcacac ctggcggatt ggggtgctta
     1321 caaaataatc agtggacaaa cttcaccacc aaaaattcag gtcttcccaa agacggtatt
     1381 ttttgcgtga ctcagaccat cacaaccgat ggaaaacgaa ccctgtgggc cggcaccgcg
     1441 ggcgggggtc tggcccggct cgatcagaat cagtggacag tgtatgacac ctccaattcc
     1501 gggttgccgg gaaatatcgt ccgcaccctg ttgccaatcc aggaaccaga cgggtcaacc
     1561 acgctctggg ttggaaccta tggtggtgga attgcccgct ttgcccgtgg tcagtggaaa
     1621 gtgctcaacc ataaaatatc tgggctgctc aatgatggta tctggacgat tggtcgcagc
     1681 ccagggccac ttggcgccac tacgctctgg attgggatgc tcagcggcct gtcacgactc
     1741 aaactcgatc aatggaaggt gtatcagccc acggctgaac aaacccagca cgtcccggtc
     1801 atgagtcttt tggaatcagt ttctggtacc caacgagccc tgtggattgg ctctgcgggc
     1861 ggaggtcttt caaaacttga gaatggtcaa tgggtaacct atgacacgca aaattcaggc
     1921 ttacccaaca accgcgtcct ggatttactt gaaaccagag gcactgacaa tagcaccacc
     1981 ctctggattg ccaccaatgg cggcggactg gccacgttga aaaatggcca gtggacggtc
     2041 tatgacacca aaaattcggg gattccccat gacctcgtct ggtccctcca accaacccga
     2101 acggttgagg gcaagccggc ggtctgggtg gcgacctatg gcggcggcat tgcgctcttt
     2161 gacgaaactg gaaaatggac ggtgctcgac acgaaaaatt ccaaaattcc gaataatctg
     2221 gtgatgtgcc tgttgttaag tcgggccgcc gatggcagtg aaacactctg ggtcggaact
     2281 gatggcgcgg gtcttgggtg ttttcgcaat ggccagtgga ccacattcaa taccagcaat
     2341 tccaggttgc caaacaatgt ggtgatgagc ctcgaagaga cggaagggcc aaacgggtcg
     2401 cgccatctgt gggttggcac cgctggcggc ggcgctgtcc gatttgaccc ggtccgacct
     2461 gccgaaccct ggctccattt gactgatact tctaacccgc cgctgcccaa taccacggtc
     2521 aatcagattt gccttgacgc tcaaaaacgg gtgtatctat ttaccaataa aggtatttcc
     2581 cggctgactc cccggtcgcc gacaactgaa aacgcctccg cctttgacct gtttacctac
     2641 acgacggaag atgggttgcc cagcatggag tgcaaccttg gcgcttcact ggttgatagt
     2701 cgtgggaata tctgggctgg aacggttgaa ggtacagtca tgctgtctcc agcgaaatct
     2761 caggaagatc gggtgcccaa accactccac ctggaacgca ttctcatcaa tggccaggag
     2821 ttgaaccaga cccaaatccg gcagtttgaa cccccaaccc ttggctatga tgaaaacaat
     2881 ctggtgtttg agtattcgct gctgtcctat atccacgaag atgcaaccac ctatcaggtt
     2941 caactggttg ggtttgatga tgcaccttct gcctggagaa aagattttaa aaaggaatat
     3001 acccgcctgc ccgccgggag ttatgaattc caggtctggg gacgtgatta tgccgggaac
     3061 attaccgaag gaccccacct gcgatttcgg atcaagcccg cgccgtggct gacgtggtgg
     3121 gcttttttga tctatgtcgg gattggagcc agtgtcgtgt atggcatgta tgaatggcgt
     3181 atgcagcaac tgtttcggcg tcaacaggag cgactggttc acctccgcca gcttcaggat
     3241 cagcgcgtca gtacattgcg ccagttgctc gaaagcattc gtgtgatcaa ctctgaactc
     3301 gatttgagca cggttctgaa aaaaattgcg gaagaaggtg cccagcttgt ggatggtgag
     3361 ccaggtggga ttggactgat tgagaaagaa acggttgtct ttcgtcctat ctggatcaac
     3421 aaggagtggc aggaagaacc attgattgtt caatttggtg aggggattgc cggaaaggcg
     3481 gcccaaaaag gccagaccgt aattgtcaac gatacttcga cgtttccgga aatggatgtc
     3541 cgcgagcggc tggcgcgcga tggtattttt ggcctgatga acgtcccgat ttattcgcgt
     3601 acgggcacaa ttgtgggcat tctggatgtg cggcggccaa aagaccgggc gccgttttct
     3661 gaatcagatc gccgattggt agagtcattt gcccatcagg cggcgattgt gatcgaaaaa
     3721 gcagcattgt atagcgaact ggaagagaaa aatttgatga ttgtcgagtc aatgcgcgag
     3781 ttgcaacggc tttacaaaca ggagcaggaa gtttcgcgca gtctgcaaga acttgaccag
     3841 atgaaaaaca acttcctgat tgtcacctcg cacgagctcc gcacaccgct tacggtcatc
     3901 agcgggtata ccgaaatcct gctggatgaa tatttagggc aagtgaatga caaacagcgc
     3961 cagtcactca aaacctgccg tgaaatgacc tcgcggatgg cagacagtct caacaatatt
     4021 ctggaaatgc tccgcatcag cgaaggctat atcacactga accagagtga acacgatctg
     4081 gcacagatga ttcgaaacat catcagcgaa ctccagggat ttgctcacct gcgcaatcaa
     4141 cgcctgctgg ctgaggtgcc cgccaccctg gttctgacga ttgacgccga aaagatcgaa
     4201 ttgctgttgc tcaacctgat tc
//
LOCUS       DAOQDO010000232         4212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14480_length_4212_cov_2.947799, whole genome shotgun sequence.
ACCESSION   DAOQDO010000232 DAOQDO010000000
VERSION     DAOQDO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14480_length_4212_cov_2.947799"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1448
                     /locus_tag="PKZ53_04985"
     CDS             267..1448
                     /locus_tag="PKZ53_04985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNJ39822.1"
                     /translation="MKPTYLAGAVRTPIGKFGGALADWTAAELGVAATQEALRRAGLT
                     PETVQEVIFGSARQAGGGPNVARQIGYRSGIPETVPAYTVNQACASGLKSILLAIQEI
                     QLGQADVVVAGGTESMSRVPYFAEGARWGARLGHTQLVDGMYRDGFLCPLSGKIMGET
                     AETLAVQYGISRREQDEFALRSHQRAEAAETAGYFAAERMTLSRTDSRGRTQTLDRDE
                     HPRAGMKIEDLAKLPPVFSKTGTVAAGNSSGITDGAAAVVVLSEEAVTTHHISPLARV
                     VGYATVGLDPNVMGLGPVPATKKLLDRQQLSLDDIDLIELNEAFAAQVIACDRELGLD
                     PERLNVNGGAIALGHPIGCTGTRIVVTLLHEMKRRQMRRGLATLCVSGGLGIALLLEN
                     V"
     gene            1562..2551
                     /locus_tag="PKZ53_04990"
     CDS             1562..2551
                     /locus_tag="PKZ53_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ39823.1"
                     /translation="MLKTRLYSALAFLIAFTIGVGLGQLPLRRATHSEIPACSHPNKL
                     SAQLTAVSISPDSRFVVSGDINGYVCLWEKRSKRLVWKNYLGGKVLSLDFSLNKPILA
                     ALTQNGHITTFNLKTGEIQLQSQPLPEIGIEGGALQYTVSDDPSAMVYGGAPLRSWAT
                     LNGRESRLHPFDSTATCITWSALSDRYALGNQSGIVKLYDKETFTEIRQWGTPFQSIQ
                     AVGLSPFGQWLAVTGDQGLGLIDLFSGNMVLPEINQPSTQNLTCIAFSKNDPILVTGS
                     QDGMISIWSIPKYSDEVTFYSSDSWYAATCSHLHSPWGCSNPNHFPLPINFAH"
     gene            2636..2785
                     /locus_tag="PKZ53_04995"
     CDS             2636..2785
                     /locus_tag="PKZ53_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39824.1"
                     /translation="MAKKCIQLISYLLLPFQVALVIGVGVIRALLPAKETKFRVSTQP
                     ASAKA"
     gene            2807..3097
                     /locus_tag="PKZ53_05000"
     CDS             2807..3097
                     /locus_tag="PKZ53_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39825.1"
                     /translation="MICHSCGRDVRILGRVGRTAECTECGADLHCCRNCRFFDTSAPN
                     QCREPIAEPEKDKAAANFCEYFEANTKIALTTRSGPTSSDSRKAFDALFKKK"
     gene            3113..3319
                     /locus_tag="PKZ53_05005"
     CDS             3113..3319
                     /locus_tag="PKZ53_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39826.1"
                     /translation="MPASTDLAYLNAVVQAIRQRPPEEALKEITEQYPTLDPVRILKD
                     LTWAKQLKQRGYSRQEILEDLHLG"
     gene            complement(3361..4089)
                     /locus_tag="PKZ53_05010"
     CDS             complement(3361..4089)
                     /locus_tag="PKZ53_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNJ39827.1"
                     /translation="MTTSSSSQRCSLQIQLFGQCRALVGASEVTLQVALPGTAALVLD
                     SLFTHHEILRPYAGKLLVAVNEAYALPTQVIQPGDEVAVFPPVSGGEPVQELTGDAYF
                     VQVTTTPIDSDAMVRRLLRGEDGAVVTFDGVVRNNTKGRRTLYLEYEGYTSMALKTMQ
                     QVAQEALNQWPIDRIGIVHRVGRLEIGESSVVIVVTSAHRKDAFDACRFGIDRVKQIV
                     PIWKREFFEDGDVWVEGQRPPELG"
BASE COUNT         1003 a         1144 c         1070 g          995 t
ORIGIN      
        1 agggttcagg gttcagggtt cagggttcag ggttcagggt tcagggttca gggtcttcga
       61 aatgagcgtg gttcaacgca atagcaccct gacaccattt tggttctttt cgtccgttgt
      121 gttccttgtg tcttttaccc tgaaccctgc tttcaaaccc ggaaccctaa accctgaacc
      181 ctgctttcaa acccggaacc ctaaaccctg aaccctgctt tcaaacccgg aacccggaac
      241 ccggaacccg gaaccctgca ttccaaatga aacctaccta ccttgctggg gccgtgcgga
      301 ccccaattgg aaaatttggc ggcgcgctgg ctgattggac agcggctgaa cttggtgtgg
      361 cggcaaccca ggaagccctc cggcgggcag gtctcacgcc agaaacggtc caggaagtca
      421 tctttgggag cgcccggcag gctggtggcg gaccaaatgt ggcgcgccag attggctatc
      481 ggtcagggat tccagaaacg gtcccggcct acaccgtcaa tcaggcgtgt gcttcgggat
      541 tgaaaagcat cttgctcgcc attcaggaaa tccagcttgg acaggccgat gtggtcgtgg
      601 ccgggggaac tgaatcaatg agccgggttc cctactttgc tgaaggtgcc cggtggggcg
      661 cccgccttgg ccatacgcaa ctggttgatg gcatgtaccg cgacggcttt ttgtgcccgc
      721 tgtccggaaa aattatgggt gaaaccgccg aaacactggc tgtccagtat ggaatctccc
      781 gtcgggaaca ggatgaattt gcactgcgct cacatcagcg ggctgaagcg gctgaaaccg
      841 ccggatattt tgcggctgaa cgaatgacac tgtcgcgcac tgattcccgt ggccgaaccc
      901 aaacccttga ccgggatgaa catccacggg ctggaatgaa aatcgaagac cttgccaaac
      961 tcccgccagt cttttccaaa accggcacag ttgcagcagg taattcaagc ggtattaccg
     1021 atggcgcggc agccgttgtt gtgctttccg aagaagctgt caccacgcat catatttccc
     1081 cgctggcgcg tgtcgtcgga tacgccacgg ttggacttga tccaaatgtg atggggcttg
     1141 ggccggttcc ggcaaccaaa aaactgcttg accgccagca actctccctg gatgacattg
     1201 atttaatcga actcaatgaa gcatttgccg ctcaagtcat tgcctgtgac cgtgaactgg
     1261 gcctggatcc cgaacggctc aatgtgaacg gtggcgcaat tgcccttggt catccaatcg
     1321 ggtgtacggg gacgcggatt gtggtcacgt tgctccacga aatgaagcga cgtcagatgc
     1381 ggcgtggcct tgcaactttg tgcgtcagtg gtggtttagg tatcgcgctt ttactcgaaa
     1441 acgtttaaga taccccgccg aagaaaattg cctgccgcga ttttcgacct ttttctcaaa
     1501 ccaccattga ccttaccaat ttgttgccgg tttcttcagc cattgaaggg gggagtacgc
     1561 tatgcttaag acacgcctgt actcggctct agcctttctg attgccttca ccattggcgt
     1621 cggcctcggt caacttccac ttcggcgggc tacccattca gaaattccag cctgttctca
     1681 tccaaacaag ctgagcgccc agcttacggc ggtctcaatt tctccagata gccgctttgt
     1741 agtctcaggg gacatcaacg gttatgtctg tttgtgggaa aaacgctcta agcgcctggt
     1801 atggaaaaac taccttggtg gaaaagtact ctcactggat ttttctctga ataaacccat
     1861 ccttgcagcc ctcacccaaa acggccatat caccaccttt aacctgaaaa ccggagaaat
     1921 tcagcttcaa tcccagccct tacctgaaat tggaattgag ggtggagcac tgcaatacac
     1981 agtgtcagac gacccgtcag cgatggtgta cggtggggcg ccattacgaa gctgggcaac
     2041 gctgaatggt cgtgaaagcc gtctccatcc atttgacagt actgcaacct gcattacctg
     2101 gtcagcgcta tctgatcggt acgcacttgg aaaccagagc gggatagtca aattatacga
     2161 taaagagaca tttactgaga tccgacagtg gggaactccc tttcagtcaa ttcaggcagt
     2221 tgggctgtca ccttttggcc aatggttggc ggtcaccgga gatcaaggat tgggattgat
     2281 tgatcttttt agtgggaata tggtgctgcc tgagatcaac caacccagca cccagaacct
     2341 tacctgtatt gccttttcca aaaatgatcc gatacttgta actggcagcc aggacggaat
     2401 gatttcgatc tggtcaattc ctaaatatag tgatgaagtg actttttatt cgtcagactc
     2461 atggtatgca gcgacctgta gtcatctaca ttcaccctgg ggttgttcga atccaaacca
     2521 ctttcctctt ccgataaact ttgctcattg aagttcacca ctcatcagta cttcaatttc
     2581 cagcaaccaa tacctgacca agagaaggtc aacggattag caatcaagcg gttttatggc
     2641 caaaaagtgt attcaattga tttcctatct cttgcttccc tttcaggtag cactggtgat
     2701 tggcgttggt gtcattcgtg cgttgcttcc tgcgaaagaa accaaattcc gggtgagtac
     2761 ccagcctgca tccgcgaaag cttaaaccac ggaggaaccg gaggatatga tttgtcattc
     2821 ctgtggccgt gacgtccgca tcctcggtcg cgtcggacga accgctgaat gcaccgaatg
     2881 tggcgccgac ctgcattgtt gtcggaattg tcgtttcttt gatacctcgg cacccaatca
     2941 gtgccgggaa ccgattgctg aacctgagaa agacaaagcc gcggccaatt tctgtgaata
     3001 ttttgaagcc aacaccaaaa ttgccttgac gacccggagt ggtccgacgt caagcgattc
     3061 acgcaaagcc tttgacgcgt tgttcaagaa aaaatgagga ggaacaggtc cggtgcctgc
     3121 atcaactgat ttggcctatt tgaacgctgt cgttcaagca attcgtcagc gcccgccaga
     3181 agaagccttg aaggagatta ccgagcagta tccaacgctc gatccggtcc gcattctcaa
     3241 agacctgacc tgggcaaaac agcttaaaca acgaggatac agccgacagg aaatcctgga
     3301 agacctgcat ctcggataat cattgggggg tgatggtttt atgaccggcg cccccgttcc
     3361 ctaccccaat tccggtgggc gttgtccctc aacccacaca tcaccatctt caaaaaactc
     3421 acgtttccag atcggcacga tttgcttcac ccgatcaatc ccaaaccgac aggcatcaaa
     3481 ggcatctttg cggtgggcag atgtcaccac aatcaccacg cttgactccc caatctcaag
     3541 ccgccctacc cgatgcacaa tcccaatccg atcaattggc cactggttca atgcttcctg
     3601 agccacctgc tgcatggttt tgagcgccat gctggtatag ccttcatatt ccagatagag
     3661 cgtccggcgg cctttggtgt tgttgcggac aaccccgtca aatgtcacca cggcaccgtc
     3721 ttcgccgcgt agcaatcgcc tgaccatggc gtctgaatca attggcgtgg tcgtgacctg
     3781 gacaaaatag gcgtctcccg tcaactcctg aacgggttcc ccgccggata ctggtggaaa
     3841 gaccgcgacc tcatccccag gctgaatcac ctgagtgggt aaggcatagg cttcgttgac
     3901 tgccaccagc agctttccag catacgggcg cagaatttcg tgatgggtaa acagtgagtc
     3961 caaaactaag gctgccgtgc ccggaagtgc aacctgaagc gtgacttcag aagcccccac
     4021 cagcgcacgg cattggccaa acaattgaat ttggagcgaa caacgttggg atgatgaaga
     4081 agtggtcatg agtagggttt cggggtttcg gggtttaagg ttcagggttt agaaaaagac
     4141 aatagttcat tgtattttcc tctctaccag cctgacaacc tgaaaaggaa tcccaatgaa
     4201 aggatggcgc cc
//
LOCUS       DAOQDO010000233         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14556_length_4200_cov_3.095778, whole genome shotgun sequence.
ACCESSION   DAOQDO010000233 DAOQDO010000000
VERSION     DAOQDO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14556_length_4200_cov_3.095778"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="PKZ53_05015"
     CDS             <1..1656
                     /locus_tag="PKZ53_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ39828.1"
                     /translation="STDALLQSLSESDAGLRRRAIDGLGKVGDARALPALQTALSDQN
                     ASVRAGAVLAFGKIGREQAIPSLLTALQETDVQVRNAAAFALGQIRTPEALKGVLTLV
                     GKSDAGQLAQNRATFDAFLKAYGESAKDVESHTKAADPVLRRGALVALDTLLNAPEAT
                     AALQAALKDSAKDIRLLAIDGLLRRKVQASGDTLLALLTSEKEADVRSQVAIALGELA
                     NRDNPRLPETLRRAHRTDASPDVREKAGLALDQLGIARLDVPGVGKEPKVAAAMPPVT
                     VSGESTLTPSRPQPPKPQPPKVEPQPVPDSKTEPAEPTTDIASSTTPTTNPVTKSPKT
                     GTATLPPAVASSKQPGKGTPSATKPASSGKETTPKIGSKPATKPADEPLMARAEPPKP
                     APTRPATKSTTASPGTNLAAKPLPPPPVASTAATRAAVIAANEAAILDVMRMLLEAEE
                     TYQAGAGEGEFGGVLDLVAENLIHEEFASGEVSGYRFSLYVSQATDHTKASFFVLATP
                     VGFEETGRRSFFMDASGMTRTRDTRNGVRLEEIFGTWPQTALR"
     gene            complement(1789..2250)
                     /locus_tag="PKZ53_05020"
     CDS             complement(1789..2250)
                     /locus_tag="PKZ53_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNJ39829.1"
                     /translation="MTEPVEMTMNPTRYGELLAQTLPVAIQNDGEYERMLAELEKLMD
                     KALRQGDEALSLEETQLFRLMTLLIEVYEQQKHPIPKAEVHEIIQFLMRDRQLRQRDL
                     LPVLGSRGVTSEIINGKRSPSKAQARALGEFFGVSPDLFVTRLESSGIERT"
     gene            complement(2289..3464)
                     /locus_tag="PKZ53_05025"
     CDS             complement(2289..3464)
                     /locus_tag="PKZ53_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HNJ39830.1"
                     /translation="MKSQSQGRLAVLIVLCVALFVVIVGVVVQQVLPEIIQHAYESQK
                     PAKSKQPQMRKPRPPSDIVPTPAIQQQHKEFSDFRTQVTDLLKQNKFAELDQLAGELR
                     KSKARFTAGGGWKIFRLYFVLDSPLDIPDGEEPTETDWQTYLKKVEGWAKQSPNSITA
                     QVLYADALTGYAWFGRGIETAPHVSDAQWKMFYERLDLAEQVLLKAKALPTKCPMWYY
                     TMQTVALGQGWDSARYDQLFTEATAFEPKFYGYYTAKAYYLLPRWYGEEGDWEAFAEE
                     TRLKVGGKEGASLYYRIAVNQSKYYKNEDFFNETNISWLLMRDGYLATEELYGRNIDA
                     LHTIAKFAFWAHDRESLKALLEKIGNDVEPECWPSQQAFNEFKDWGMGKTEKMPVVK"
     gene            3744..>4200
                     /locus_tag="PKZ53_05030"
     CDS             3744..>4200
                     /locus_tag="PKZ53_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNJ39831.1"
                     /translation="MRKDAIEFFTSRYHITHDDLNKLLAEALGKGGDYADLYFEYRQS
                     NSVSLEEQIVKTANQSISQGVGVRVNVGEKTGYAYTDEISVESIRRAAQTAACIAKET
                     ATSASVNVSARPNAHDLYAIPVAISELPVEHKIEMIKQIDQKARAYDRRI"
BASE COUNT         1027 a         1111 c         1089 g          973 t
ORIGIN      
        1 tcaaccgatg cgttgctgca atcactttct gaaagcgatg ccgggttgcg ccgccgggcc
       61 attgacgggc ttgggaaagt cggtgatgcc agagcgttgc cggcgctcca aacggcgttg
      121 agcgatcaga acgccagtgt gcgagccggt gcggtgctgg cctttgggaa aatcggtcgg
      181 gaacaggcga ttccaagttt gctcactgcg ttacaggaaa ctgatgtcca ggtccgcaat
      241 gcggcggcct ttgccttagg gcagattcga acccccgaag cgctcaaagg tgtgttgacc
      301 ctggttggaa aaagcgatgc cggtcaactg gctcaaaacc gggccacatt tgatgccttc
      361 ttgaaagctt atggtgaaag tgccaaagat gttgaatcgc acaccaaagc tgcggacccg
      421 gtactgcggc gcggggccct ggtcgcgctt gatacgttgc tgaatgcgcc tgaagcaacc
      481 gccgcactcc aggcggcgtt gaaagattcg gctaaagaca ttcgtctgct ggcaattgac
      541 ggactccttc ggcgcaaagt tcaggccagc ggagacaccc tgcttgcgct tctgaccagt
      601 gagaaagaag ccgatgttcg cagtcaggtg gcaattgctc tgggtgaact ggccaatcga
      661 gacaatccac gattgccaga gacccttcgg cgagcacatc gaacggatgc cagccccgac
      721 gtgcgtgaaa aagccggtct ggcgctggac cagcttggca ttgcccggct ggatgtgcca
      781 ggggttggaa aagaaccaaa agttgcggcg gcaatgcctc cggtaacggt ttcaggtgaa
      841 tctacgttga cgccgtcgcg gccacaaccg cccaaaccgc agccgcccaa agttgagccg
      901 cagccagttc ctgactcgaa aactgaaccc gccgagccaa caactgacat tgcaagttca
      961 accacgccga ccacaaatcc ggtgaccaaa tcacccaaaa ccggcaccgc caccttgccg
     1021 ccagccgtgg caagttcaaa acaaccaggg aagggaacgc cgtcagcgac caaaccagct
     1081 tcgtctggaa aagaaaccac accgaaaatc ggatccaaac cggccaccaa gcctgctgat
     1141 gagccgctga tggcgcgggc tgaaccgcca aaaccagccc caacccgtcc agcgaccaaa
     1201 tcaacgactg cttcacctgg aaccaacctg gcagccaaac cacttccgcc gccacccgtt
     1261 gcctcaacgg cagccacccg agcggcggtg attgctgcca atgaagccgc cattctggat
     1321 gttatgcgaa tgttgcttga agccgaagaa acctatcagg cgggtgctgg cgaaggtgaa
     1381 tttggtggtg tgcttgatct ggtagctgaa aatctgattc acgaagagtt tgcgtcgggc
     1441 gaagtcagcg gctatcggtt ttcgctctac gtgagtcagg ccactgacca caccaaagca
     1501 agtttctttg tcctggcaac gccggttggg tttgaagaaa ctggacgccg gtcatttttt
     1561 atggatgcct ccgggatgac ccgcacccgc gacacccgca atggtgtccg attggaagaa
     1621 atcttcggca cctggccgca aacggcattg aggtaggtgg ggctgaaaga cagcgggctg
     1681 aagaatctgg gctgaagact cgcaagctca gggctgaaga aatcgggcgg aagaatctgg
     1741 gctgaagcca gcactgaagg ccgattcttc cgcttttgtt cctgtctttt atgtcctttc
     1801 aattccagag ctttccaacc tggtgacaaa cagatcaggt gaaacgccaa aaaactcacc
     1861 cagcgctctg gcctgggctt tactcgggga acgcttgcca ttgatgattt cagatgtcac
     1921 gccgcgtgac cctagcaccg gcagtaagtc acgttgtcgc aactggcggt cacgcatcag
     1981 gaattgaatg atttcatgaa cttcagcttt cggaatcgga tgtttttgtt gttcatacac
     2041 ctcgataagc agcgtcatca atcggaacaa ttgggtttcc tcaagtgaca acgcttcatc
     2101 accttgacgg agcgccttat ccatcagctt ttcaagttcc gccagcatcc gctcatattc
     2161 gccatcgttt tgaatcgcta ctgggagagt ttgggccaac agttctccat atcgggttgg
     2221 gttcattgtc atttcaaccg gttcagtcat tcgaaatctt ccacacacca aaattcttcg
     2281 gtaacccatt atttcacaac cggcattttc tcggttttgc ccattcccca atctttgaac
     2341 tcattaaatg cctgctggct tggccagcat tccggttcga catcatttcc aattttttca
     2401 agcagggctt taagggattc acggtcatgt gcccaaaaag caaatttcgc aatggtgtgc
     2461 aatgcatcaa tattgcgacc ataaagttcc tcggttgcaa ggtaaccatc acgcatcaac
     2521 aaccaggaaa tgttagtttc gttgaagaag tcttcatttt tgtaatattt ggactgattc
     2581 accgcaatgc ggtaataaag tgatgcgcct tctttgccgc cgactttgag gcgggtttct
     2641 tcggcaaatg cttcccaatc accttcctcc ccataccagc gcgggagcag gtaataggct
     2701 ttagcggtat agtaaccata aaactttggt tcaaaggcgg ttgcttcggt aaatagctgg
     2761 tcatatcgtg cggaatccca tccttgccca agtgccactg tttgcatggt gtagtaccac
     2821 attgggcatt tcgttgggag cgctttggct ttcagcagca cttgttcggc aaggtcgagt
     2881 cgttcataga acattttcca ctgggcatcg ctgacgtgag gagctgtttc aattccacgg
     2941 ccaaaccagg catacccagt cagtgcatcg gcatagagca cctgcgccgt gatcgagttg
     3001 ggagattgtt ttgcccatcc ttctactttt ttgaggtatg tttgccagtc cgtttcggtc
     3061 ggttcctcac catcaggaat atccagtggg gaatcaagta caaaatacag tcgaaagatt
     3121 ttccaaccac cgccagcggt gaacctggct ttggatttac gcaactcgcc agcaagctgg
     3181 tcaagctcgg caaatttatt ctgcttgagc aggtcagtta cctgtgttct gaaatccgaa
     3241 aattccttgt gttgttgttg aatggcagga gttggaacga tatcagacgg cggtcttggt
     3301 ttccgcatct gaggttgttt ggatttggct ggtttctggg attcatatgc gtgctggatg
     3361 atttctggaa ggacttgttg caccaccact ccgacgatga cgacaaacag ggccacgcac
     3421 aaaacgatca aaaccgcgag acggccttgg gattgggatt tcataccttt ctccttgaaa
     3481 aagaaagagt taccatcttt gcagacgtga tctgcacagt ggcaagtatt tagtcgctta
     3541 agttgaatgt gattctgctc agtgtagcgc agtcaaatgt tggattccag aacgaaaaac
     3601 agttttgaat caaaccagca ttgatttcat acttgcttga cgacgacacc tggtgatcag
     3661 gcaaattcgg ctttgcgaat ggtgcagaac agacaataat ggcgcttcct gaatttcatc
     3721 cagaaaaagg taggctgcac catatgcgga aagatgcaat tgaattcttt acctcccgat
     3781 atcacatcac ccacgatgat ttgaacaaac tcctggctga agcgctcggc aagggtggag
     3841 actacgcgga cctgtatttt gaataccgtc aaagcaacag tgtgtcgctc gaagagcaaa
     3901 tcgtcaaaac cgccaaccag tccatttcgc aaggcgttgg cgtgcgggtc aacgtcggtg
     3961 aaaaaaccgg ctatgcctac accgatgaaa tcagcgttga gagcatccgg cgggcggccc
     4021 aaacggcggc ctgcattgcc aaagaaacgg cgaccagcgc cagcgtcaac gtgtcggcac
     4081 gtcccaatgc ccacgattta tatgcgattc cggttgccat cagtgaactt ccggtcgaac
     4141 acaagatcga aatgatcaag cagattgacc agaaagcccg cgcctatgat cggcgcatcc
//
LOCUS       DAOQDO010000234         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14602_length_4193_cov_2.748188, whole genome shotgun sequence.
ACCESSION   DAOQDO010000234 DAOQDO010000000
VERSION     DAOQDO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14602_length_4193_cov_2.748188"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1361)
                     /locus_tag="PKZ53_05035"
     CDS             complement(111..1361)
                     /locus_tag="PKZ53_05035"
                     /inference="COORDINATES: protein motif:HMM:NF021632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HNJ39832.1"
                     /translation="MGDAGPPCVIHVACPPCREQDGRKDMEQQNSIIEEIERKRPVDV
                     AKIERELAELWKAAASSKEGGGEAVTRVCLLNLLIYTSDEDQAATLGATIATVTEHAP
                     CRAMVLVGNLKNPDSHTEAWISSHCHLTQNRQQVCCEEIRVTSSGKALEHLSDTIAPL
                     LVADLPTFLWWQDAHALSQPLFQGFLKQVNRIIVDSGQLTHRNQEFRHQAEIILNSRH
                     QAAFSDLNWARLTVWEELIASFFDAPEMHVYLKRMNKVTITYSFNGELEGAPIRTLFL
                     VSWLANRLSWKLKDAVEVSPQHLEFRFRSPYTRLQLEIVPETNTNFPSTVITAVTLST
                     AGEKPATFEVRRRAPKEKEDVFETHVVITDGASVDRVVSSHRPHDADLIAHEVEIFGR
                     DRAYEEAVKSTLSLIEEIQAELLV"
     gene            complement(1383..2924)
                     /gene="zwf"
                     /locus_tag="PKZ53_05040"
     CDS             complement(1383..2924)
                     /gene="zwf"
                     /locus_tag="PKZ53_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNJ39833.1"
                     /translation="MSTLDTLENPLREGMRLETSPAPNTVVIFGASGDLAKRKLVPAL
                     YNLARDHRLPGGFSVVGFARSESSHDAYRTDMREAVAKFSTTGPPEDSLWDSFAQGLF
                     YCTGNYDDLASYQRLSTLLDQLDAERGTGGNRVFYLSTPPSLISPIMDHIGKSGLSKG
                     KDGGWARIIIEKPFGHDLASAQHLNQEVNRIFDEDQVYRIDHYRAKETVQNIVVMRFA
                     NGIFEPIWNRNFIDHIQITAAEAVGVEGRGGYYENAGAVRDMLQNHLLQLLALVAMEP
                     PPSLDSTAIRDENIKVVKAIRPIPADRVNEFAIRGQYSRGWVGGAEAPGYRQEPGVNP
                     ESATETFAATKFYIDNWRWADVPFYMRSGKRMTKRATEIAVQFRNVPHRLFNQTAADL
                     IEPNLLVIRVQPDEGITIKLAAKVPGQAMHLRSVNMDFRYGSSFGVRSSDAYERLILD
                     CMLGDATLFTRRDMVEAGWALMMPVLESWQAAKDKDLHFYQAGSWGPEAADSLMLEGR
                     SWRRL"
     gene            complement(3076..>4193)
                     /locus_tag="PKZ53_05045"
     CDS             complement(3076..>4193)
                     /locus_tag="PKZ53_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006669299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39834.1"
                     /translation="LNVQQSMSMKVPFGIAQIGKSFRNEITTERFIFRTCEFEQMEME
                     YFVEPGAQVEALKYWTETRMSWWKQFANDGSKFRLRQHETDELAHYADNCYDVEYLYP
                     WGWDELEGIASRTNYDLTKHAQESGVKLSYFDQQKNDPETGKPLVDLVMDKAGQKGTG
                     KWTSEEALEMGVAVSTIHASIEARCLSALKDERVEANSQIPGPADYVYEGDRQAFIDA
                     VHDALYASKICSYAQGMAMLAQASRERNWNLNLGEISRIWKGGCIIRAQFLDKIKQAY
                     HRRADLPNLLLDPDFKAWVVDAQPRWRQVVSVATLAGIPIPAMSASLGYFDMYRTANL
                     PLNLTQAQRDYFGSHTYERIDQPDRGFIHTEWKSLMK"
BASE COUNT          891 a         1074 c         1229 g          999 t
ORIGIN      
        1 aagaaccaag aaccaagaac caagaaccaa gaaccaagaa ccaagaacca agaatgtttc
       61 ttcattctca attcttcatt ctcaattctt cattcttcat ttgattgggg tcagaccagt
      121 aattcagcct gaatctcttc aatcaggctg agtgtggatt ttacggcttc ttcataagcc
      181 cgatcacggc caaaaatttc aacttcatgg gcaatcaggt cggcatcgtg ggggcggtga
      241 gaagaaacaa cccgatcaac ggaggccccg tcagtgatga cgacgtgggt ttcaaagacg
      301 tcttcttttt cttttggcgc ccgacgccgg acttcgaagg tcgccggttt ttcaccggct
      361 gttgacaggg tcaccgccgt gatgacggtg gaggggaagt tggtgttggt ttccggcaca
      421 atttcaagtt gcagccgggt atagggagat cggaatcgaa attccaggtg ctggggcgaa
      481 acttccacgg cgtctttgag tttccacgag aggcgattgg ccagccagct caccaggaac
      541 aatgtgcgga tgggggcgcc ctcaagctca ccattaaaag agtaggtgat ggtgactttg
      601 ttcatgcgtt tgagatagac gtgcatttcc ggcgcatcaa aaaagctggc aatcaattcc
      661 tcccaaaccg tgagacgggc ccagttcaaa tcgctaaacg ccgcttgatg gcgggaattg
      721 aggataatct cggcctggtg gcgaaattcc tgatttcggt gggttaactg tccgctgtca
      781 acaatgatgc ggttgacctg tttgagaaat ccttgaaaga gcggctggct cagggcgtgg
      841 gcgtcctgcc accagaggaa ggtcggcagg tcggcgacga gcagaggggc aatcgtatcc
      901 gaaaggtgtt ccaacgcttt tccactggac gttacccgga tctcctcgca gcagacctgt
      961 tgtcggttct gggtcaggtg acagtgggac gagatccagg cttcggtatg gctgtccggg
     1021 tttttcaggt ttccgaccag caccatcgcc cggcaggggg catgttcggt gacggtggca
     1081 atggtggcgc ccagggtggc tgcctgatct tcatccgacg tgtaaatcag caggttgagc
     1141 aggcacaccc gggtgacggc ttcaccacca ccttcttttg agctggccgc ggctttccag
     1201 agttcagcca gttcacgttc aattttggcg acatcgaccg ggcgcttgcg ttcgatttct
     1261 tcgataattg agttttgctg ttccatatct tttcgtccgt cttgttcgcg gcagggaggg
     1321 caggcaacgt ggatgacgca gggcggcccc gcgtcaccca cggccaaaca cctgcatcca
     1381 ggttagagtc ggcgccagga gcggccttcc agcatcaaag aatcagccgc ttcggggccc
     1441 cacgagccgg cctgatagaa gtgcaaatct ttgtctttgg cggcctgcca ggactccagg
     1501 accggcatca tcaatgccca gccagcttcg accatgtcgc ggcgggtaaa gagagtggca
     1561 tcgccgagca tacaatcgag aatgagtcgt tcgtaggcat cagacgagcg gacaccaaag
     1621 gaactcccat atcgaaagtc catattgact gaccggaggt gcatggcctg acctggaact
     1681 ttggcggcca gtttgatggt gatgccttca tctggctgga cccggatgac cagcaggttg
     1741 ggctcgatca gatcggcagc cgtctggtta aacagccgat ggggaacatt gcggaactgg
     1801 acggcgattt cggtggcccg cttggtcatg cgtttgccgg aacgcatata aaacggaaca
     1861 tccgcccacc gccagttatc gatgtagaac ttggtggcag caaaggtttc ggtggctgat
     1921 tcagggttga caccgggttc ctggcgatag cctggcgctt cggcacctcc aacccatccc
     1981 cggctgtact ggccgcgaat ggcaaattcg ttgacccggt cggctggaat tggccggata
     2041 gcctttacga ccttgatgtt ttcgtcgcgg attgcggttg agtccagtga gggaggtggt
     2101 tccatcgcca ccagggccag gagctggagc aggtggtttt ggagcatgtc acgtacggcg
     2161 ccggcatttt cataatagcc gccgcggcct tccacgccga cggcttcggc ggcggtgatt
     2221 tgaatatggt caatgaaatt ccgattccag atgggttcaa aaatcccgtt ggcaaaccgc
     2281 atcaccacga tgttttgaac cgtttctttc gcccgatagt ggtcaatgcg atagacctga
     2341 tcttcatcaa agatccgatt gacttcctgg ttgaggtgtt gggctgaggc cagatcgtgg
     2401 ccaaacggtt tttcaataat aatgcgtgcc cagccgccat ccttgccttt ggagaggccg
     2461 gatttcccga tatggtccat aatcgggctg atcaggctgg gtggggtgga gagataaaaa
     2521 acgcgatttc cgcccgtgcc ccgctcggca tcaagctggt cgagcagggt gctcaggcgc
     2581 tggtaactgg ccagatcatc atagttgccg gtgcagtaaa acaggccctg ggcaaagctg
     2641 tcccacaggg agtcttcggg cggcccggtg gttgagaatt tagcgactgc ctcccgcata
     2701 tcggtgcggt aggcatcgtg ggaggattcg ctgcgggcaa agccgaccac cgaaaagccg
     2761 ccgggaagcc ggtgatcacg tgccaggtta tacagtgccg gcaccagttt gcgtttggcc
     2821 aggtcgcccg aggccccaaa aataacgacc gtattggggg cgggtgaggt ttcaagccgc
     2881 ataccttcgc ggagtggatt ttccaaggta tccaaagtgc tcatatcttt tcaccttctc
     2941 taattgtgag tgaatagcga gtcgcggaga gaaagcaagt cgcgaggagc gaggtgtcaa
     3001 tcagatctct ggattcaatc ttgaactgga tcactgaatt accaggggtg actccttgct
     3061 cctcgcgcgc tctggctact tcatcaatga cttccactcc gtgtggataa agccacggtc
     3121 aggctggtca atgcgttcat aggtgtggga gccaaagtag tcgcgttggg cctgggtcag
     3181 gtttaatggc aggttggcgg tgcggtacat gtcaaagtac ccgagactgg cggacatggc
     3241 tgggatgggg atgccggcca gggtcgcgac gctgaccacc tgtcgccagc ggggttgagc
     3301 atcgaccacc caggctttga aatcggggtc gaggagcagg ttgggcagat cggcccgacg
     3361 gtgatatgcc tgtttgatct tgtcgaggaa ttgcgcccga ataatacagc cacctttcca
     3421 gatgcggctg atttcaccca ggttgaggtt ccagttccgc tcgcgtgagg cctgagccag
     3481 catggccatt ccctgggcat atgagcagat tttgctggcg tacagggcat catgaacggc
     3541 atcaatgaag gcctggcggt cgccttcata gacgtaatca gccggacctg gaatttgtga
     3601 gttggcttcg acgcgttcat ctttgagggc tgagaggcag cgggcttcga tggaggcgtg
     3661 aatggttgag acggcgacgc ccatttcgag ggcttcttcg gacgtccatt tgccggtgcc
     3721 tttctggccg gctttgtcca tcaccaggtc caccagcggt ttgccggtct ctggatcatt
     3781 cttctgctgg tcaaaataac tcaactttac gcctgactct tgggcgtgtt tagttaggtc
     3841 gtaattggtg cgtgatgcta taccttcaag ctcatcccag ccccacgggt agagatattc
     3901 tacgtcgtag caattgtcag catagtgagc cagctcgtct gtttcgtgtt gtctcagtct
     3961 aaatttgctt ccatcattag caaattgctt ccaccatgac atgcgcgttt cagtccaata
     4021 ttttaaggct tcaacctgag cgcccggttc aacaaagtac tccatctcca tctgctcaaa
     4081 ttcgcaggtg cggaaaataa agcgctctgt tgtaatttcg tttctaaatg atttgccgat
     4141 ctgggcaatg ccaaatggca ctttcatcga catgctttgt tgtacgttaa gaa
//
LOCUS       DAOQDO010000235         4189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14621_length_4189_cov_4.013788, whole genome shotgun sequence.
ACCESSION   DAOQDO010000235 DAOQDO010000000
VERSION     DAOQDO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14621_length_4189_cov_4.013788"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKZ53_05050"
     CDS             <1..670
                     /locus_tag="PKZ53_05050"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNJ39835.1"
                     /translation="YGPNVPIMLGVNDEPTIVGRRILPREAHGIRFMSIGLINRGDRP
                     VIMRGPMLHGVVQQFLREVEWGELDYLVVDMPPGTGDVQLSLAQLVPVSGAVLVTTPQ
                     EVALADVRKAFNMFRQVGIPVFGIVENMSYFVCPHSAEPIFIFGQGGGAKMAKDFDST
                     FLGEVPLSINVRQGGDVGVPVVIGEPESIQAQAFFKIAEALAAQVSIAAIEAPKLPTL
                     NIGG"
     gene            673..894
                     /locus_tag="PKZ53_05055"
     CDS             673..894
                     /locus_tag="PKZ53_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39836.1"
                     /translation="MVQVAITEDNRFSTFVKSKKSEAYRDLEMWQEWFAARGIPSVIA
                     STSSGYALYREGLIEVDIHDDRPASAVAA"
     gene            1315..2073
                     /locus_tag="PKZ53_05060"
     CDS             1315..2073
                     /locus_tag="PKZ53_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNJ39837.1"
                     /translation="MTFVFPQIWAHRGARSQAPENTLAAFRRAFELGADGIELDVMCC
                     GTGELVVFHDYEVSRLTTGKGKTGELSLQALKSLDVGSHFSPQFAGEPPPTLPEVLDA
                     LPPTASINIEIKNEAIRSAGEEQIVVKLIRQYRLFDRAIVSSFNPFVLRRVRKLDPHI
                     RIGYLHEAHPRLYLRPKAVSTWLRPNAVHPEFTVVTSEMIRQAHQRGVAVHAWTVNEV
                     EVMRRLIEWGVDALITDFPERALKLKAEYLTGNE"
     gene            2286..3458
                     /gene="sat"
                     /locus_tag="PKZ53_05065"
     CDS             2286..3458
                     /gene="sat"
                     /locus_tag="PKZ53_05065"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNJ39838.1"
                     /translation="MISPHGGRLIHRVATPEVRAELLDRAASLPRLTLGSREISDLEM
                     IADGALSPLEGFMNRADYESVRDHMHLASGLPWSIPITLSATTEQAAQFNEGQEIALY
                     NGDDELLAVMNLEEKSPYTQADKEREAEKVYRTTDAAHPGVKAVYDMGDVLLAGPITL
                     VNHLTGLPFADHRRTPAQTRAAFAERGWERVVGFQTRNPVHRAHEYIQKVALEIVDGL
                     MLHPLVGDTKSDDIPADVRMKCYQVLLDNYYPQNRVLLSVFPAAMRYAGPREAIFHAI
                     VRKNYGCTHFIVGRDHAGVGNYYGTYDAQLIFKEFDARALDITPLFFEHTVFCRKCGS
                     MVSPKTCPHGNEHHVFLSGTKVREMLQRGEIPPVEFTRPEVAEILIESMRSASATA"
     gene            3582..>4189
                     /locus_tag="PKZ53_05070"
     CDS             3582..>4189
                     /locus_tag="PKZ53_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNJ39839.1"
                     /translation="MKKYFGTDGIRCVAGEFPLQPRALNVLGATLVTEFSHRLGRQPS
                     FVVGGDTRESTSWIAGALGWGIRSAGGKLVSAGVIPTPGVAYLTSAMKFDAGVVISAS
                     HNPFQDNGIKIFFPSGQKQDNALETALELALESLAHEMPDQTDSGESLDLRTDDNLSA
                     RYLEFLCSEIGKGLSLSKLKLAVDCAHGAAVDVLLPRKDRCGV"
BASE COUNT          957 a         1102 c         1059 g         1071 t
ORIGIN      
        1 ttatggcccc aacgtgccaa tcatgttggg tgtcaacgat gaacccacga ttgttggtcg
       61 tcgcattttg ccccgcgaag ctcacggaat tcggtttatg tcgattggat taatcaatcg
      121 cggagaccga ccagtcatta tgcgcggccc aatgctgcac ggtgtcgtgc agcaatttct
      181 tcgggaagtt gaatggggag aactggatta tctggtggtt gacatgccgc ccggaaccgg
      241 agatgtccag ttgagcctgg cccagttggt gcccgtttca ggtgccgtgc tggtcacaac
      301 tccccaggaa gtagccctgg ctgatgttcg caaggcattt aatatgtttc gccaggtcgg
      361 gattccagtt tttggaatcg tcgaaaacat gagctacttc gtctgtccac actctgccga
      421 accgattttt atcttcggac agggtggcgg tgccaaaatg gcgaaggatt ttgacagtac
      481 ttttttgggt gaagtgcccc tgagcataaa cgttcgccag ggcggtgacg taggggtacc
      541 tgtagttata ggcgaacctg agagtattca ggctcaagca ttcttcaaaa tcgccgaggc
      601 gcttgcagct caagtgagca tcgccgcaat cgaagcgccc aagctgccta cactaaacat
      661 tggagggtaa tcatggtgca agttgccatt acggaagata atcgtttttc cacattcgtc
      721 aaaagtaaaa aatcagaagc ctatcgtgat ctggaaatgt ggcaggaatg gtttgcagcc
      781 cgtggaattc catctgtcat tgcaagtact tcaagcggtt atgccttata tcgcgaaggt
      841 ctgattgaag ttgatatcca cgacgataga ccagccagcg ctgttgcggc ctaatccttc
      901 ggaaccctat ctcaccgggt gcggtgcttt tcctcttgtt caggccacag gcccttcgtt
      961 tcgttgaaaa gtgactggct ccctttgaag aaatatcaac cccgcacgag gcatcccgat
     1021 gtactggttt catcaggatg cctcgacttg ttttatatag ggttgaagac atcggacttg
     1081 aggctgaaga catcgggccg aagacatcgg gctcggggct gaagacgacg ggctgaagac
     1141 actgggctaa ggacttcgca agattgagat tggggttacc cgctttcttc agccctgagc
     1201 ttgcaaatct tcagcccgtt ttcttcagcc cgaagttttc agccctgagc ttgcgaatct
     1261 tcagcccgtt ttcttcagcc cccagcccgc tttcttcagc ccttttctta cactatgact
     1321 tttgtttttc cacaaatttg ggctcatcga ggggcgcgct cccaggcgcc ggaaaacaca
     1381 ctggcggcgt ttcgtcgggc ctttgaacta ggggccgatg gcattgaact ggatgtgatg
     1441 tgttgtggga caggcgaact ggttgtgttt catgattatg aggtttcacg gctcacaaca
     1501 ggcaaaggca aaaccgggga gctttctctt caggccctga aatctctgga tgtcggcagc
     1561 catttcagcc ctcaatttgc tggtgaacct ccaccaaccc tccctgaagt tttggatgcc
     1621 cttcccccaa ccgcatcaat taacatcgaa atcaaaaatg aagccattcg gagtgctggt
     1681 gaggaacaga ttgtcgtgaa attgattcgc cagtaccgcc tgtttgaccg cgccattgtt
     1741 tcatcattca acccatttgt gctgcggcgg gtacgcaaac ttgacccgca cattcggatt
     1801 gggtatctcc acgaagccca cccacggttg tatctccgcc ccaaagcagt ttcaacctgg
     1861 ctccggccaa atgcggtgca tccagaattt accgtggtta cttcagaaat gattcgacag
     1921 gctcatcaac gcggagttgc ggttcatgcc tggactgtta acgaagttga ggttatgcgt
     1981 cgcttgattg agtggggtgt ggatgcgttg attactgatt ttccggaacg cgccctgaaa
     2041 ctcaaagctg aatacctgac cggaaacgag taacctcgtt cccagagtct gtgcttgctg
     2101 atcggttatc acccctcgac agcggctcac aatcggccat ctgcccgctc agttaaaatc
     2161 tcacacttca aaggcaccac ggtggcttgt ctcactttcc ttttggagaa tccccctgct
     2221 cgtgatacaa agatcagttt ttctctgttg atttaattga aagaaaggaa tctttgaaac
     2281 atcgtatgat ttcacctcat ggaggtcgcc tcattcacag agttgctaca cccgaagttc
     2341 gtgccgaact acttgaccgg gcagcttccc ttcctcgctt gacccttggt tcacgtgaaa
     2401 tttcagattt ggaaatgatt gctgatggtg cattgtcacc gctcgaaggt tttatgaacc
     2461 gcgctgacta tgaaagtgtc cgcgatcata tgcatctggc atctgggctt ccgtggtcaa
     2521 ttccgattac tctttcagcc accactgaac aagccgctca gtttaacgaa ggccaggaaa
     2581 tcgccctgta caatggcgat gacgagttgc tggcagtgat gaacctggaa gaaaaatctc
     2641 cctatactca ggcagataaa gaacgagaag ccgaaaaagt ctatcgcacc actgatgccg
     2701 ctcaccccgg cgtcaaagcg gtctatgata tgggagacgt gttgctggcc gggccaatta
     2761 cgcttgtcaa tcacctgact ggccttcctt ttgctgatca tcggcggacg ccagctcaaa
     2821 cccgagccgc ttttgccgaa cgtggctggg aacgtgtggt cggattccaa acgcgcaatc
     2881 cggtccaccg ggctcatgaa tacattcaaa aagttgccct tgaaattgtt gatggcctga
     2941 tgctccaccc actggttggt gacaccaaat ctgacgatat tccagcggat gtccgcatga
     3001 agtgctatca ggtattgctc gacaattact atccacaaaa ccgggttctg ctgtccgtct
     3061 ttcccgccgc catgcgctac gccgggccac gcgaagcaat ttttcacgcg attgtccgca
     3121 aaaactatgg ctgcacccat tttattgtgg gtcgcgacca tgcgggcgtg ggcaattact
     3181 atggcacgta tgacgcccag ttgatcttta aggaatttga tgcccgtgca ctggacatca
     3241 ccccgctgtt ctttgaacac acagtttttt gccgcaaatg tggcagcatg gtttccccaa
     3301 aaacctgccc acacggaaac gaacatcacg tgttcctgtc aggcaccaaa gttcgcgaaa
     3361 tgctccagcg aggcgaaatt ccaccggttg aattcacccg ccccgaagtt gccgaaatcc
     3421 tgattgagtc aatgcgaagt gccagcgcaa cagcctaatc tgtattcgta cctggtgctt
     3481 tgtacctggt gcttggaaag tattggttcc taagcactaa gtacaaagca ccggatacaa
     3541 aacaccagac acctcatcct ctcctacgtt ctatccactc tatgaaaaag tactttggaa
     3601 ccgatggtat tcgctgtgtg gccggtgaat ttccattgca accacgcgcc ctgaatgttt
     3661 tgggggctac cctggtgaca gaattttctc accgacttgg acggcaaccg tcttttgtcg
     3721 ttggtggtga tacccgagag tccacatcgt ggattgctgg cgccctgggt tggggaattc
     3781 ggtcagcagg tggaaagctg gtctcagccg gagttattcc aacgcctggt gtcgcgtatt
     3841 tgacttccgc gatgaaattt gacgccggtg tggtgatctc agcttcgcac aacccctttc
     3901 aagacaacgg gatcaagatt ttcttcccaa gcggccagaa acaagataat gccctggaaa
     3961 cggctctgga gctggcactt gaatcgcttg cccatgaaat gcccgatcag actgacagcg
     4021 gggagtcact tgaccttcga accgatgaca acctttcggc acgctacctt gaatttctct
     4081 gttctgaaat tggaaagggg ctttcactgt caaagctaaa actggctgtg gattgtgctc
     4141 acggcgccgc cgttgatgtc ctgctgcccc ggaaagaccg ctgcggcgt
//
LOCUS       DAOQDO010000236         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14703_length_4176_cov_3.195341, whole genome shotgun sequence.
ACCESSION   DAOQDO010000236 DAOQDO010000000
VERSION     DAOQDO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14703_length_4176_cov_3.195341"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..2320)
                     /locus_tag="PKZ53_05075"
     CDS             complement(134..2320)
                     /locus_tag="PKZ53_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ39840.1"
                     /translation="MKQCPECKRVFRDDEMVCPDHGQYLTPQMILDGKYRLEEKIGEG
                     GMGEIYRATHLRLGTTWAVKILNLDLLSDESSVQRFWREAQVSTQINHPNVVNVVDIV
                     ESKECGVMYIVMEYLAGKNLKERLYEGHFLTWEEIHLVLRQICNVLDCAHAKGIIHRD
                     LKPDNIFLVDTPEIARSVKVLDFGIAYVGKDMRLTMTGELMGSPEYMSPEHCEDRDMD
                     YRADIYTLGVVLYELLTGDVPFSLLRYGFQTIMVKHLTELPRRIRDIRPDVPEAVEAV
                     VLKALSKNPNQRQQSASQLAQEFEEALITSGIHVKELSFPLLQTQSTSAMPDVMGAPS
                     PWSLSPLTQESPTSLTNPGTETLDPFTSPLAAPLTKEDLDPVTDPADSLTKEDLDPVT
                     DPADDMIVEPAETIEMNPGPALTEDFLLPSVTQEVNPGHDPLATNPIHTAAREGMLPL
                     RTVSFEVVTLNTFGQVVFQTKRQAEIFDEPFVSGAFLEMIEIPSGSFEMGDAGLDAEQ
                     PVHKVSVPTFFISKFAITQAQWHEVSTWPKVERELLPAPSKFSGPMLPVEQITWDDAM
                     EFCARLAKKTCRSYRLPTEAEWEYACRAGTVSPFAFGETLTAEIVNYNGEYPYGQAPK
                     GENRRKPIPVGSLHIPNAFGVYDMHGNVMEWCLDVWHPNYIDAPADGSARRHTDDQRY
                     RVVRGGSWNHSAHDCRSAYRYGRLPDLKNSYLGFRVVMTLSDWWRA"
     gene            2407..3741
                     /locus_tag="PKZ53_05080"
     CDS             2407..3741
                     /locus_tag="PKZ53_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ39841.1"
                     /translation="MPSFTVVGSGLAGCLMAVYLAQRGYKVTLFERNDDMRDRPVPRG
                     RSINLALSRRGINALQEVGIGDKLMELAIPMRGRMIHSPTGQLTFQPYGKNDQEAIYS
                     ISRNALNVALINLAETYPNVEVFFNHKCLGADLNRTTITLEDQITKEVKTVSAEMVVG
                     ADGAYSAIRDTMQVRTERFDYSQQFLSHGYKELTIPALPDGSHALDKNALHIWPRHDF
                     MMIALPNLDGTFTCTLFFPHQGPMSFESLKNPEEVRRFFTLHFPDAVPIMPTLVEDFF
                     HNPTGAMVTVRCAPWHYRGRFVLTGDAAHAVVPFYGQGMNASFEDCSVLNACMDMHGE
                     DWPAVFEAYQSRRKRNTDVLSELAIANYLEMRDKVASPWFLMKKKLDNVLATLLPGVY
                     LPLYTMVSFTLIPYADAVERAHKQDRILLMVGIGILLLPLLLAGWWVLRSLG"
     gene            complement(3983..>4176)
                     /locus_tag="PKZ53_05085"
     CDS             complement(3983..>4176)
                     /locus_tag="PKZ53_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39842.1"
                     /translation="DETESTPVSTAAVTAEAPSRRTAELPPPVYDTNHFMNNPPSSVT
                     ESPTRTFEEKPRIQKQERQ"
BASE COUNT          924 a         1077 c         1058 g         1117 t
ORIGIN      
        1 caccagtcac caagaactac tttcttcatt cttcattctt cattcttcat tcttcattct
       61 tcattctgca ttcttcatcc ttcattctgc attcttcatc cttcattctt cattcttcat
      121 tcttcattct tcttcaagct cgccaccagt cactcaaggt catcaccacc cgaaatccca
      181 gatagctatt tttcagatcc ggaagccggc catagcggta ggcagagcgg caatcatggg
      241 cgctgtgatt ccacgaaccg cctcgtacca cccggtatcg ctgatcgtcg gtgtggcggc
      301 gggcactgcc atcggctggg gcatcaatgt agtttggatg ccacacatcc agacaccatt
      361 ccatgacatt tccgtgcatg tcatagacac caaacgcatt ggggatgtgt agactgccaa
      421 cggggatggg tttgcggcga ttttcgccct ttggtgcctg accataggga tattcgccat
      481 tgtagttgac gatttctgcc gtgagggtct ctccaaaagc aaaaggcgag actgttcccg
      541 cccggcaggc atattcccac tcagcttcgg tcggcagacg atagctgcgg caagtttttt
      601 tggccagacg ggcacaaaac tccatggcat cgtcccaggt aatttgttca actggcaaca
      661 tggggcctga aaacttcgaa ggtgctggca gcaattcccg ttcaactttt ggccaggttg
      721 aaacttcgtg ccactgcgcc tgggtaatgg caaatttact gataaaaaag gttggtaccg
      781 atactttatg cactggctgt tcggcatcga gcccggcgtc tcccatctca aaactgcccg
      841 atggaatttc aatcatctcc agaaaagccc cactgacgaa cggctcatca aaaatttcag
      901 cctgccgttt ggtttgaaac acaacctgtc caaaggtgtt gagggtcacg acttcaaatg
      961 aaactgttcg aagcggcaac atcccttcgc gggcggcggt atgaatggga ttggtggcca
     1021 gcgggtcgtg cccagggttt acttcctggg tgacagatgg caacagaaaa tcttcagtca
     1081 gcgccggccc tggattcatt tcaatggttt cagccggttc aacgatcata tcgtctgccg
     1141 gatccgtcac cgggtctaaa tcttcttttg tcagggaatc tgctggatcc gtcaccgggt
     1201 ctaaatcttc ttttgtcagg ggagcggcca gcggagaggt aaacggatcc aaggtctccg
     1261 tcccaggatt ggtcaggctg gtcgggcttt cctgggtcaa gggggagaga ctccacgggg
     1321 atggtgcccc catcacatca ggcatggcgc tggttgattg ggtttggagt aatggaaacg
     1381 acagttcttt tacgtgaatg cctgaagtga tcaacgcctc ctcaaactcc tgggcgagtt
     1441 gtgaggctga ttgctggcgc tggtttggat ttttgctcag tgcttttaag acaaccgcct
     1501 cgacggcttc gggaacatcg ggccgaatgt cgcgaatccg gcgtggaagt tcagtcaggt
     1561 gcttgaccat aattgtttgg aacccgtagc gaagtaacga aaacggcacg tctccggtaa
     1621 gtaattcata gagcaccacg cctaatgtat agatgtcggc gcgataatcc atgtcacgat
     1681 cttcgcaatg ctcgggagac atatactctg gcgagcccat caattcgccc gtcatcgtca
     1741 accgcatgtc cttccccaca taggcaatgc caaagtcgag tactttgact gaccgggcaa
     1801 tctccggggt atccaccaaa aatatattat ctggctttaa atcccgatgg atgatccctt
     1861 tggcgtgagc acaatcgagc acattgcaaa tctgtctgag gaccaggtgg atttcttccc
     1921 aggtcaaaaa gtgcccttca tagagacgtt ctttcaggtt ttttccagcc agatactcca
     1981 tcacaatgta catcacgccg cattctttcg attcgacgat gtccacgaca ttgaccacat
     2041 tcggatgatt gatctgggtt gagacctgag cttcacgcca gaatcgctga accgaagatt
     2101 catcggaaag caaatcgagg ttgagaattt tcacggccca ggttgttccg agacgcagat
     2161 gcgtggcacg atagatttcg cccatgccgc cttcgccgat cttttcctcc agccggtatt
     2221 tcccatccag aatcatttgt ggcgtcaggt attgcccatg gtcaggacat accatctcgt
     2281 catcgcgaaa cactcgttta cactcgggac attgcttcat ttttctgagg tgctccgaaa
     2341 ccgaaattaa gtggggtgtg ctatattctt tcgcttcact tcgactgtca aacaggagtt
     2401 ttttccatgc cttcttttac tgtggttggc tcagggttag ccgggtgcct gatggcggtt
     2461 tacctggctc aacgtggtta caaagtgacg ctttttgaac gcaacgacga catgcgagat
     2521 cgcccagtcc cacgaggtcg ctcaatcaat ctcgcgcttt cccgacgtgg aatcaatgcg
     2581 ttacaggaag ttgggattgg cgataagttg atggaactgg cgattccgat gcggggacgc
     2641 atgattcatt ccccaaccgg gcaactgacg tttcagcctt atgggaaaaa tgaccaggaa
     2701 gcgatttatt cgatttcacg caatgcgctc aatgtcgccc tgatcaatct ggcggaaacc
     2761 tacccaaacg ttgaagtatt ttttaaccat aaatgccttg gggcagatct caaccgcaca
     2821 acgattaccc ttgaagacca gataaccaaa gaagtcaaaa ctgtttcagc cgaaatggtg
     2881 gttggtgctg atggggccta ttcggccatt cgcgacacca tgcaggtccg aaccgaacgc
     2941 tttgattata gccagcagtt tttgagccat ggctataaag aactcacgat cccggcactt
     3001 cctgatggca gccatgcgct tgataaaaat gcactgcata tctggccacg ccacgatttt
     3061 atgatgattg cgctgccgaa tctggatggg acgtttacct gtacgctctt ttttccacat
     3121 caggggccga tgagctttga atcgctcaaa aacccagaag aggtacggcg tttctttacc
     3181 ctccatttcc cagatgccgt acccattatg ccgacactgg ttgaggattt cttccataat
     3241 ccaactggtg cgatggtcac ggttcgatgt gctccgtggc actatcgggg acggtttgta
     3301 ttgacgggtg atgctgccca cgcggtggtt ccgttttacg gccagggaat gaatgcttca
     3361 tttgaagact gttcggtgtt gaatgcctgt atggatatgc acggcgaaga ctggccagcc
     3421 gtttttgaag cctatcagtc acgccgcaaa cgaaacaccg atgtcctgtc cgaactggcc
     3481 attgctaact atctggaaat gcgggacaaa gtggcctcac cctggttttt gatgaaaaag
     3541 aaactcgata atgtcctggc tactctgttg ccaggggtgt atctcccgct ctacaccatg
     3601 gtttccttca ccttaattcc ctatgccgat gccgtggaac gagcccacaa acaggatcga
     3661 attttgttga tggtgggaat cggaatcctc ttgctgccgc ttttgctcgc tggttggtgg
     3721 gttttgagga gcctgggctg aagatcttgg gctcagggct tggggctgaa gatcttgggc
     3781 tgaagaagtc gggctgaaga agtcgggctg aaaacttcgg gctgaagaat tggtcttatt
     3841 tcatacctcg attgccccga gcttgcgagt cttcaacccg ttgtcttcaa cccgttgtct
     3901 tcagcccgtt gtcttcaacc cgttgtcttc aacccgttgt cttcaacccg ttgtcttcag
     3961 cccgttgtct tcaaccccac tctcactgcc tttcctgttt ttgaattcgg ggtttttctt
     4021 caaacgtgcg agttggggat tcggtcacgc ttgatggcgg gttgttcata aaatggttgg
     4081 tatcatagac cggaggcggg agttcagccg ttcggcggga tggggcttcg gcggtgacgg
     4141 cagccgttga tacaggcgtt gattcggttt catctt
//
LOCUS       DAOQDO010000237         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14725_length_4172_cov_7.518824, whole genome shotgun sequence.
ACCESSION   DAOQDO010000237 DAOQDO010000000
VERSION     DAOQDO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14725_length_4172_cov_7.518824"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PKZ53_05090"
     CDS             complement(<1..1214)
                     /locus_tag="PKZ53_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNJ39843.1"
                     /translation="MKQIVQNFKTGELLVEELPPPALKPGGVLVHTAYSLISVGTERT
                     TVETGQSSLIGKALKRPDLVKQVWNTFRREGLRSTYEKVTARLEATKALGYSSAGIVL
                     EVGDGIDEFRPGDLVACAGVGYASHAEINFIPRNLCTRIPDGVGLDAAAYTTLGAIAM
                     QGVRQAKIELGSCVVVIGLGLLGQITVQLLKAAGCRVFGIDLSPRVLELAKKSGADAV
                     CLRSDDTLKAQCEAFTHGRGADAIILTASTEKADPVELAGELARDRARVVVVGAVKMD
                     IPREHYFRKELELCLSRSYGPGRYDPVYEEGGIDYPIGYVRWTERRNMEEFLRLVANG
                     AVKTDFLTSHRFSVERARDAYDVILGKTNEPVCGVLIEYPKAPAGTPPVRKLLTAGAA
                     GKPKSGDIGVGF"
     gene            complement(1312..1638)
                     /locus_tag="PKZ53_05095"
     CDS             complement(1312..1638)
                     /locus_tag="PKZ53_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ39844.1"
                     /translation="MASFQQLTPQEVKARLDAGDDIVLLDVRERQEFEFNRLEGAVFR
                     PLSQIHTWVNQLDKTKEYVVYCHHGMRSQQACMFMYHNGFQKLNNLYGGIDLWSHDVD
                     PTVPVY"
     gene            complement(1811..3328)
                     /locus_tag="PKZ53_05100"
     CDS             complement(1811..3328)
                     /locus_tag="PKZ53_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ39845.1"
                     /translation="MSTSTDAAAPATAHKTSLETEFAKGLGLFDSMMVVVGVMIGSGI
                     FIVSADMARLLGSPGWLLAAWVVTGILTIAAALSYGELAAMMPRAGGQYVYLREAFSP
                     MWGFLYGWTLFMVIQCGTIAAVGVAFARFLGVLWSPVSETSYLIPPIHITSGYAVSLS
                     SAQLVGILVIVFLTWMNSRGLEYGKIVQNIFTITKTGALLGLILLGLLIGWNATAANA
                     NFGNLWTPRSFTPIVPGLDVTTTFGLFVGICVAQVGSLFSADAWNNITFTAGEVRNPQ
                     RNLPLSLALGTLIVISLYLLANVAYLAVLPLEQIQNAPSDRVATATINAIFPGMGAMV
                     MAVAIMISTFGCLNGIILAGARAYYAMAKDGLFFAAAGRLNSAKVPGWALMIQGVWAA
                     TLVLPRTFDVSTKTYGNLYGNLLDYIVSAAVLFYILTILAVFRLRQTRPNAERPYKTV
                     GYPFVPALYLIGASVILVVLFIYRPATTWPGLLIVLSGVPIYAFLKNRAGLVKNG"
     gene            complement(3511..3969)
                     /locus_tag="PKZ53_05105"
     CDS             complement(3511..3969)
                     /locus_tag="PKZ53_05105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ39846.1"
                     /translation="MIIRQALASDANKLTAIAHAAKRYWNYPEHWLAAWESSLTITPE
                     FIARHPIFVADEDHQILGFYALTRDESDWFLEHCWVRPESIGTGIGRQLFEHAIEMAC
                     AAGMTSLLIDADPFAAPFYERMGARQIGTIPAPVPEQPDRTLPRMQFKVV"
BASE COUNT         1025 a         1109 c         1143 g          895 t
ORIGIN      
        1 atgaacccaa cgccgatgtc accggatttg ggtttccccg ctgcacctgc cgtgagcaat
       61 tttctgactg gcggtgtccc agccggagct tttggatatt caatcaggac gccgcagacg
      121 ggttcgttgg ttttgcccag aatcacatca taggcgtcgc gggcgcgttc gaccgaaaac
      181 cgatgcgagg tcaagaagtc ggttttgacg gcgccgttgg ccaccaaccg gaggaattcc
      241 tccatattgc ggcgttcggt ccagcgcaca taaccgatag ggtaatcaat tccgccttct
      301 tcatagaccg ggtcatagcg gcctggtcca tatgagcgcg agagacagag ttcaagttcc
      361 ttgcggaaat agtgttcgcg aggaatgtcc attttgacgg caccgaccac gacgacgcgg
      421 gcccggtcgc gggcaagttc cccggcaagt tcaaccgggt cagccttttc cgtgcttgct
      481 gtgaggatga tggcatcggc gccgcgcccg tgggtgaagg cttcacactg ggctttgagc
      541 gtgtcgtcac tgcgcaggca gacggcatcg gcgcctgatt tcttggccaa ttcgagtacc
      601 cgtggggaaa ggtcaatgcc aaacacccgg cagccagcag ctttgagcag ttgcacggta
      661 atctggccga gcaatcccag cccaatcacg accacacagg aaccaagttc aattttggcc
      721 tgacggacac cctgcatcgc aatggcaccc agcgtggtgt aggccgccgc atccagcccg
      781 acaccgtctg ggatgcgggt gcagaggttg cgcggaataa agttaatctc agcgtgtgag
      841 gcatagccga caccagcaca ggctacgagg tcgccaggac gaaattcatc aattccgtcg
      901 ccgacttcaa ggacgattcc ggcagagctg tacccaaggg ccttggtggc ttcgagccgg
      961 gccgttactt tttcataggt gctgcgcagt ccttcgcgcc gaaaagtatt ccagacctgt
     1021 ttgaccagat cagggcgttt gagggctttc ccaatcaggg aactctggcc ggtttcgacc
     1081 gtggtgcgtt cagtgccaac gctaatgagt gagtaagcgg tgtggaccag cacaccaccc
     1141 ggtttcaagg caggaggggg aagttcttcg accagaagtt ccccagtttt aaagttttgg
     1201 acgatttgtt tcattcgatt agggctaagg gttgagggct gagggctaag gggtttcaac
     1261 cagtgaaatc tggtattttc taccctcagc cccacaccct cagccctctt tttagtagac
     1321 cggtaccgtg ggatcaacat cgtgcgacca gaggtcaatc ccgccataca ggttgttcaa
     1381 tttttggaaa ccattgtggt acataaacat acacgcctgc tggctgcgca tgccgtggtg
     1441 gcaatacacc acatattctt tggttttatc aagctggttg acccaggtgt gaatctggct
     1501 caacgggcga aagaccgcgc cttcaaggcg gttaaattca aattcctgtc gctcgcgcac
     1561 gtcgagcaac acgatgtcat cgccagcatc aaggcgggct ttgacttctt gaggtgtgag
     1621 ttgttgaaaa ctggccatag gatgagggtt cagggttcag ggttcagggt tcagggttta
     1681 gggtttaggg ttcagggttc tggtaaaaag caaggacaaa agggatgaaa aggacataaa
     1741 ttcgaaaacc cggaacccgg aacccggaac ccggaacccg gaacccggaa cccggaaccc
     1801 ggaacccaaa tcagccattc ttcaccaaac cggcccgatt cttcaagaag gcatagatgg
     1861 gaacaccgct caatacaatc aggagtcccg gccaggtcgt ggccgggcgg tagataaaga
     1921 gcaccaccag aattacgctc gcaccaatca aatacagcgc tggaacaaat ggatacccga
     1981 cggttttgta tggtcgctca gcatttggac gtgtctggcg caaccggaac accgctaaaa
     2041 tggtcaggat ataaaacaac actgccgccg acacaatgta atcaagcagg ttcccataga
     2101 gattgccgta ggttttggtc gaaacatcaa acgttcgcgg caacaccagc gtcgccgccc
     2161 aaactccctg aatcattagt gcccaaccgg ggactttggc gctattgaga cgtccagccg
     2221 ccgcaaaaaa caggccgtct ttggccatgg cgtagtaggc ccgcgctcca gccagaatga
     2281 ttccattcag acagccaaag gttgaaatca taatcgccac ggccatcacc atggcgccca
     2341 tgcccgggaa aatggcgtta atggtggcgg tcgcgacacg gtcagacggt gcattctgga
     2401 tttgctccag cggtaacacg gccaggtagg cgacattggc cagcagatag agcgaaatca
     2461 caatcagcgt gcccagtgcc agtgaaagtg gcaaattccg ttgtggattg cgcacttcgc
     2521 cagccgtaaa cgtgatgttg ttccaggcgt cggctgagaa gagcgaccca acctgggcga
     2581 cgcaaatccc gacaaagagg ccaaaggttg tcgtgacatc cagcccagga acaatcggtg
     2641 tgaaactgcg tggtgtccag agatttccaa aattggcatt cgccgccgtg gcgttccagc
     2701 caatcagtaa gcccagcaaa atcaatccca gcaaggcacc agttttggtg atggtaaaga
     2761 tattctggac gattttccca tattccaacc cacgcgaatt catccaggtc agaaagacaa
     2821 tcaccaggat tccaaccagt tgggctgagg aaagagatac cgcatacccg ctcgtaatgt
     2881 gaatcggtgg aatcagatag ctggtttctg acactggaga ccacaacaca cccagaaatc
     2941 gagcaaaggc cacgccgacc gccgcaatcg tcccacactg aatgaccata aacagcgtcc
     3001 agccatagag aaacccccac attggcgaaa acgcttcacg caaatacaca tattgtccac
     3061 cggctcgtgg catcatggca gccagttcac cataagacag ggctgcggca attgtcagaa
     3121 tgcccgtaac gacccaggcc gccagcaacc agccggggct gccgaggagt cgtgccatat
     3181 ccgccgaaac aatgaaaata ccggagccaa tcatgactcc gaccaccacc atcatcgaat
     3241 caaagagtcc gagacctttg gcaaattctg tttctaaaga tgttttgtgg gcagttgccg
     3301 gggctgccgc atcggtcgaa gtggacataa tgttgtggtt ccttccgagc agaatgtgaa
     3361 tttttgaaga cagcgaaaaa gttcagagtt caagctttag cctgcctgct ggttcaggat
     3421 caggtaaccc agggggaatc aagagtttaa atttcgcgat gggaagatct ttgccagtta
     3481 aagcttgaat tctgagtaaa agatatcttt ttagactact ttgaactgca ttcgcgggag
     3541 tgttcggtcg ggctgttcgg gaactggggc tggaatggtg ccaatttgcc gggcacccat
     3601 gcgctcataa aacggagctg caaatgggtc ggcatcaatc agaaggctgg tcattccagc
     3661 ggcgcaggcc atttcaatgg cgtgttcaaa caactggcga ccaattcccg tgccgatgga
     3721 ttctggtcgc acccagcaat gttccagaaa ccagtctgat tcatccctgg taagtgcata
     3781 aaaaccaagg atttgatggt cttcatctgc cacaaaaatc ggatggcgag cgataaattc
     3841 cggcgtgatg gtcagtgagg attcccacgc cgccagccag tgttccggat agttccagta
     3901 ccgtttggca gcgtgggcaa tggcagtgag cttgttggcg tctgaggcaa gtgcctggcg
     3961 aataatcatc agcgtatggg gccagggttt tagggttcag ggttcagggt tcagggttca
     4021 gggtttcgaa aatttagtcc ttttggtccc ttatgccctt gatgtccttt gatctaaccc
     4081 ggttcccatc tggcgtcaac caactttctg aaacagcccg cacacggcac ctgccgggtc
     4141 gcggatgaca caatatttcc cgtgttcgcc gg
//
LOCUS       DAOQDO010000238         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14745_length_4170_cov_3.136817, whole genome shotgun sequence.
ACCESSION   DAOQDO010000238 DAOQDO010000000
VERSION     DAOQDO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14745_length_4170_cov_3.136817"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(513..2579)
                     /locus_tag="PKZ53_05110"
     CDS             complement(513..2579)
                     /locus_tag="PKZ53_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ39847.1"
                     /translation="MTNPPPSGAFERFAQALLPLPEFPAFHIERLRRKLFYVVSALAV
                     VIPLLEIESLLYSAVTPKFALLLVGAALLVTLMGRYALRERQLSAIRTPICFLVGGLV
                     LMIGISALFSLSPIISLVGSLDIRTGFLTYACYLACFLALTISLTTDRTWLFRFFQIL
                     VLPGLGVSLLGFYAYVHQSTAPNTADPLLFRLKSTLGHADYAGNFLLFTLFAALAATL
                     VSIKPLWKWINLAAAAVILGAILFTGTRGAWAGLSVAGVAGLILVFRERRTFFQSLSP
                     QQVRQGWIVTGCLLLVFGGMVWFTPVGHTVRTRLAGTVQEGFTGAGRTTLWKWALRVV
                     PDYWATGCGPEMYRLACLNYITADDARATSGLAVEDPHNLILSYLVNYGVFSTVMLLV
                     IVGLTFVSGWQALKKSTTFQEKGVSLLLWLALLAVTVDLLFIYFTLTTGLYFFGLIAI
                     MFVWNQQPGNESAPAPSIRPTQNRATPRHKPVPSREVLVWGLLAIPVLAAVIYLFPVV
                     AAEYTIYQSMDAARQKNTQRCLELGQKAFSYGLYQTDHHYYFARALEAITVLIPDSDQ
                     DFFLTQAAAEAEKALPHCLFPDQYRFFQARWLLQADNIFEADAISDEIEAINPHYFGI
                     HVIRAQVALRLEQLDRAQTEIAAAKALNGPRRYISPLNKELKQARGISRPAKPREKKK
                     SNPGSH"
     gene            complement(2739..3956)
                     /locus_tag="PKZ53_05115"
                     /pseudo
     CDS             complement(2739..3956)
                     /locus_tag="PKZ53_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
BASE COUNT         1081 a         1009 c         1124 g          956 t
ORIGIN      
        1 tcatcgcaac cacgcaacac agcgcaaaac acagggcgat ctgccagggg gaaattttga
       61 gatgggaagt gattttttcg ggcatgctgt ccactcctgg gataaaggta atagaacaag
      121 ccaccccaaa tcctgattca cttttttgga gaacgtgcct gagggagtga aacagggtat
      181 gtggttgcag gaacaggaac tagaaggttg gattggtgct ataaatagaa caatatgtgt
      241 gagttaggcc ctgcaaatat acactctgaa tcagccagcg tcaaacgaat gttttttagc
      301 gaatgggtat gatttatgat caaggttcac tttttttgag tttgtatttt attgataatt
      361 ctagggtttt tggatagttg gtttttaggt aaaagttaga tttattcgtg aagccagccg
      421 tccatgcagg ttgatatcct ccccatggtt ccaaaaaatt gaactaatcg tgtggaaggg
      481 gatcatattt tttgggttct gaaccctcgg ggttagtggg aacccggatt ggacttcttc
      541 ttctctctgg gtttggccgg gcgggaaatg cctctggcct gtttgagttc tttgtttagt
      601 ggactgatat atctgcgagg gccattgagg gctttggccg ccgcaatttc agtttgggcc
      661 cgatcaagtt gttccaatct gagcgcgacc tgggcccgaa tcacatgaat gccaaaataa
      721 tgtggattga tggcttcaat ttcgtcactg atggcatctg cttcaaaaat attgtcggct
      781 tgcagtaacc accgcgcctg aaaaaagcga tattgatctg ggaagaggca atgaggaagc
      841 gctttttcag cttcggcagc cgcctgggtc aagaaaaagt cctggtcact gtctgggata
      901 aggacggtga tggcctccag cgcacgcgca aaataataat gatggtcagt ttgatagagc
      961 ccgtaggaaa aggctttctg gccaagttcg agacaccgct gggtattttt ctggcgtgcg
     1021 gcatccatgc tttgatagat ggtgtattca gccgcgacca cagggaaaag atagatcacg
     1081 gctgccagca ctggtattgc cagcaggccc caaaccagaa cttcacgtga tggaaccggt
     1141 ttgtgacggg gtgtcgctcg gttctgggtt ggccgaattg atggagccgg ggctgattcg
     1201 tttccaggtt gttgattcca gacaaacatg atggcgatga gcccaaagaa atacaggccc
     1261 gtggtcaagg tgaaataaat gaacagcaag tcaaccgtaa ccgccagtaa tgcaagccat
     1321 agcaggagac tcacgccctt ttcctgaaag gtggttgatt tcttcaatgc ctgccagccg
     1381 gaaacgaagg tcagtccgac aatgaccagc agcatcaccg ttgaaaacac cccgtagttg
     1441 accagatagc tcaggataag gttgtgagga tcttcgacgg ccagtccgga ggtcgcccgg
     1501 gcatcgtcgg cggtaatgta atttaaacag gcgaggcgat acatttctgg tccacacccg
     1561 gtggcccagt agtcaggcac gacccgcaat gcccatttcc agagcgttgt ccgacccgca
     1621 ccagtgaagc cttcctgaac tgtacccgcc aggcgggttc gaaccgtatg cccaactggg
     1681 gtgaaccaga ccattcctcc aaacacaagg agcaggcacc cggttacaat ccagccctga
     1741 cgaacctgtt gcggtgaaag actttgaaag aaagtgcgtc gttcccgaaa caccagaatc
     1801 aagcccgcaa ccccggctac actgagcccg gcccaggccc cgcgtgttcc ggtaaacaaa
     1861 attgccccaa gtatcactgc cgctgccgcc agattgatcc atttccagag tggtttgatg
     1921 ctgaccaggg tggctgccag agcggcaaac agggtgaata acagaaaatt cccggcatag
     1981 tcagcgtgcc caagggttga tttcaggcga aacaacagtg ggtccgctgt atttggcgcc
     2041 gtggactgat gcacataggc atagaatcca agcagggaaa cgccaagacc cggaagcacc
     2101 aggatttgaa aaaatcgaaa aagccaggtg cgatcagttg taagcgaaat cgtaagcgcc
     2161 agaaaacacg ccaggtaaca ggcatatgtc aaaaaacctg tccggatatc aagcgatcca
     2221 accagggaaa tgattggtga aagcgaaaac agggccgaga tcccaatcat caaaaccagt
     2281 cctcccacca ggaaacagat gggggttcga atggcactca actggcgctc acgcaaggca
     2341 tatctgccca tcagcgtgac caggagcgct gcaccgacca gtaacagcgc aaacttagga
     2401 gttactgccg agtacaacag gctttcaatt tccagtaatg gaattaccac ggccaatgcc
     2461 gacacgacat agaagagttt tcggcgcaga cgctcgatgt gaaaggcagg gaattcgggc
     2521 aacggcaaca gggcctgggc aaagcgctca aaggcgccag atggaggtgg attcgtcata
     2581 gttgggcgtg agactgagaa aaccagggct gaagactttg ggctgagggc tgagaagacg
     2641 agggctgaag actcgcaagc tcggagcaat tgaagggatg aaggatgaag gatgaaataa
     2701 aaccaggtct tcagccccag gccctgagcc cgttttcttc agcccgatgt tttcagtcgg
     2761 ttcagcagtt cttccttgtc gtctcgataa aacatgttga aggtatcaaa cgggatgatc
     2821 cgtccgtccg ggtggacaat gtggacgcag ctttttttga cggatcggac gtcgaagtta
     2881 aaaggatcta aaaactgcat aatcaggatc cggaagacat tttcataccc aatcgagtca
     2941 ggcacctgaa ccagcggcag acaacagagc agttgtttaa gcgacagcgc ggatgattgt
     3001 ggtgaatgcg acgtcgaaaa gagcttgaag acgtggtctt tcaccgctgg atcctgttca
     3061 tacacaatgg tattgctgcc gtgctggagc aacaccgtcg gatcaatcag actcgtcagc
     3121 gggactgcct gaccattgat tttcagggca taggccatgg ccagacagtc gggatgacag
     3181 ggaaccggga tcatatctgc cggagagagc cacgaacatt gtttgagaat ttgctggcga
     3241 acttcgccaa gtgtcaaccg atctcgcgct ggatcaaagt tttctacccg cccggcgctc
     3301 tgaattggct gaaacgtgac acctcggaca cagggctgtt gcagtgcaaa ctcaacgatc
     3361 tcgccgattt cctcgtcatt caatcctttt tttaacgtga ccaccagtgt ggttgacagg
     3421 ttatagcggt tgagtcggtc cagtgctcgc cggtggactt cgcgtaaatc gaggccgcgc
     3481 agttctttga ttggctctgc ccggagcgaa tcaaactgca ggtaaatctc aaaatcgggc
     3541 atgtattcgc tcaggcgtct ggcaaattct tcatcctggg caatccgaat gccgttggtg
     3601 ttaaccatca cgtgtttgat agggcggctt ttcgcggcat cgaggatttg aaagaactcc
     3661 ggatgaatcg ttggttcgcc gccactgatc tgaacaatat cgggctcgcc ttcattgcgg
     3721 acgacgcaat caagtaagaa ttcaacttgc tccagcgaac gccacgtctg acgatgtggc
     3781 cccgattcgg cataacaaat cggacattgc agattgcact gatcggtaat ttcaaccagc
     3841 gtcaggcaac tgtgctgctc atggtcaggg cacaatccgc aatcgtaggg gcatccatac
     3901 ttgatcggcg tattgaattt cagcggcatc tgtcctggtt tcaaatagtt gcgcgacttg
     3961 ctggacctat caccacgcaa cgctgcttac ttaaagtctc tgagcccgcg ttgctttccc
     4021 taatcatttc aggtaatacc ccgactccaa ttttagcccg gtgttttttt ggagtagcga
     4081 gtacacgtga tcccgaactc tgacgaatca agcagcaaga agactctttc aacgactgaa
     4141 ggtggagcag cagcttcatc cgcaaacctt
//
LOCUS       DAOQDO010000239         4169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14750_length_4169_cov_3.099417, whole genome shotgun sequence.
ACCESSION   DAOQDO010000239 DAOQDO010000000
VERSION     DAOQDO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14750_length_4169_cov_3.099417"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PKZ53_05120"
     CDS             complement(<1..1356)
                     /locus_tag="PKZ53_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNJ39848.1"
                     /translation="MSETPQPNVMPPPGGPQLTLPGLDAIDPKALAQPAAPDRSDFDI
                     ADAEAKNAQELARRYRMEFVDLGALNLDYDLINSLPVELMVRHQFIPLRRDPHRLIIA
                     MADPTNMDLLDELAVQLKTQLKPAVATKSAIENALKRGDTAQRVLQGATESFRMKLVK
                     ETEQGEQDLDLDRLTDETSPVIKLIDTIILNALERRVSDIHIEARDTEVMVKYRIDGA
                     LYPAAKPIDITYHPMLISRIKVMSELDIAERRVPQDGRFRVRIKGRTVDFRVSIIPAA
                     HGESCVIRILDREQINESFKDLSLNIVGFEEHDLRRFRKFIAEPYGMVLVTGPTGSGK
                     TTTLYAALNEIYNEEDKIITIEDPVEYQLRGVTQIPVNEKKGLTFARGLRAILRHDPD
                     KVMVGEIRDAETAQIAINAALTGHLVFTTVHANNVIDVIGRFLNIGVEAYNFVSSLNC
                     VL"
     gene            complement(1424..2641)
                     /locus_tag="PKZ53_05125"
     CDS             complement(1424..2641)
                     /locus_tag="PKZ53_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNJ39849.1"
                     /translation="MPEFTCRLGTPAGELITKVVEADGAEELKLRLEREGFRVFAIDL
                     PGANLGGIFSFSTQGSVKLEEFFLFNQQFATLLRAGLPMLQALGVLVRRLKPGMFRTV
                     LEDVERRIRSGASLSEALTAHPKVFPRLYTASILAGERSGELDRVLLRYINHAKVMTE
                     LRRKLKKTLMYPVILITASAGLVGLLTTYVIPKFATLYESSNTKLPLITEYVVGISKY
                     TQENLAWIAPLVLVAGVAFTLWKRTPAGELAIDRFLLKLPVMGDVIRQTTISRMARSM
                     ATLLAGGLTLLESLEISTEVVSNRVLSDSMVQVMRQIREGQSLIDSLEQAGWVPAMAM
                     DMIGVGEKSGSLGTMLDEVAVFYDAELDLRLSTLTTLIEPIVLIFMAAVVLTVLLALY
                     LPILQFVGDRPTQ"
     gene            3037..4023
                     /locus_tag="PKZ53_05130"
     CDS             3037..4023
                     /locus_tag="PKZ53_05130"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ39850.1"
                     /translation="MSTQRLSNPTPVSGWKETTARWFKRAGIAMLGAAGSYMLLSAAA
                     AYPLSSLLVTPQKKRLAQLTSRHLRYFLRRKRVHYQEVDFHSFDGTRLHGWFLQAGRW
                     KPTVIALHGVTGNRTSMLRFAMVLYQAGFNVFVFDGRGHGLSQGEFVTYGYHETRDIS
                     ALIDYLIREHKVREKSIGLIGMSMGAAIALQVAARDKRIQAVWAESPFSSLEQISAEY
                     IADATRLPQSMLKPVTWGAMFVANRRGRFNVSEVSPVAIAPHITCPVQLVHGAADMFV
                     RPEHSQRIFTALTTLPKELWLVPRAAHTQCFRYARQEYRERLEWFFRKNLGR"
BASE COUNT         1008 a         1037 c         1129 g          995 t
ORIGIN      
        1 cagtacacag ttgagcgaac tgacaaagtt ataggcttcg accccgatat tgagaaaccg
       61 gccaatgacg tcaatgacgt tgttggcgtg aacggttgta aacacaaggt gtccggtcag
      121 agccgcgttg atcgcaattt gagccgtttc agcatcgcgg atttccccaa ccatgacctt
      181 gtccgggtcg tggcgcaaga tggcgcgcag tccacgggca aaggtcagtc ctttcttttc
      241 attcaccgga atctgagtca ccccgcgcaa ctgatattcg accggatctt caatggtgat
      301 gattttatct tcttcgttgt aaatttcatt gagggcggca tagagggtgg tggttttgcc
      361 agagccggtt ggcccggtta ccagcaccat gccatagggt tcggcaatga acttgcggaa
      421 ccgccgcagg tcgtgttctt caaacccgac gatgttgagg ctcaaatctt taaaggattc
      481 gttgatttgc tcgcggtcca gaatacgaat aacacagctt tcaccgtggg ccgcgggaat
      541 gatcgaaacc cggaaatcaa ctgtgcgccc tttgatccga acccggaacc gtccgtcctg
      601 gggaacgcgc cgctcggcaa tatcgagttc cgacatcacc ttgatgcgcg agatcagcat
      661 cgggtgatag gtaatatcaa ttggtttggc tgccggatac agtgccccgt caattcggta
      721 cttgaccatg acctcagtat cgcgagcttc aatatgaatg tccgagaccc ggcgttcaag
      781 tgcatttaaa ataatggtat caatgagttt aatcaccggc gacgtttcgt cggtcagccg
      841 atccaggtca aggtcttgct caccctgttc tgtttccttg accagcttca tccggaatga
      901 ttcggtcgcg ccttgcagga cccgttgggc ggtatcaccc cgtttgaggg cattttcaat
      961 cgctgacttg gtggcaaccg ctggctttaa ctgggttttt aactgaaccg ccagttcatc
     1021 gagcagatcc atattggttg gatcagccat ggcaatgatt aagcggtggg gatcacgccg
     1081 gagcggtata aattgatgcc ggaccatcaa ttcgactggc aggctattga tcaggtcata
     1141 gtccagattg agggcgccga ggtcaacaaa ctccatccgg tagcgccgag ccagttcctg
     1201 agcatttttg gcctcagcat ccgcgatgtc aaaatctgac cgatccggtg cggcgggttg
     1261 tgccagcgcc tttggatcaa tggcatcaag tcctgggagc gttaattgtg gcccgccagg
     1321 tgggggcatc acattgggtt gtggcgtttc agacatagca aatacagagt tctctcaaaa
     1381 tgtgaagagc aacggatgac catcaatttt agaatccgga cacttactga gtcggacgat
     1441 caccaacaaa ctgcaggatg ggcagataga gcgcgagcaa gacggttaac acgacggcgg
     1501 ccataaaaat caacacgatt ggttcaatca gggtggttaa ggttgagagc cgcaggtcaa
     1561 gctcggcatc gtaaaaaacc gccacttcgt cgagcatggt tccgagtgat ccagatttct
     1621 cgcccacgcc gatcatatcc atcgccattg ccggtaccca tccggcctgt tcgagtgaat
     1681 caatcagtga ttgaccttcg cgaatttgtc gcatgacctg gaccatcgaa tcagacagca
     1741 cgcggttgga taccacttca gtcgaaattt ccagggattc caggagcgtc aaccccccgg
     1801 ccaggagtgt agccatgctg cgggccatgc gggagatggt tgtctggcga atcacatcgc
     1861 ccatcactgg aagcttgagg agaaagcgat caatggcgag ttctccggcg ggggtccttt
     1921 tccacagggt aaaggccacc ccggcaacga gaaccagggg ggctatccac gccaaattct
     1981 cctgggtgta ttttgagatc ccaaccacat actctgtaat cagcggcaac ttggtatttg
     2041 agctttcata gagtgtggca aatttaggaa tgacataggt ggttaacaac ccgaccagtc
     2101 ctgccgaagc tgtgattaaa ataactggat acatcagtgt tttctttagt ttacgacgca
     2161 attccgtcat cactttggcg tgattgatat agcgcagcaa cactcgatcc agctcgcctg
     2221 accgttcacc agccaggatg gaggccgtat acaaccgggg aaacactttt gggtgggcgg
     2281 ttaatgcttc agacagcgaa gccccactcc gaatacggcg ttcaacatct tccaaaacag
     2341 tccgaaacat cccaggtttt aagcgtcgaa ccagcacgcc cagtgcctgc aacataggta
     2401 atccggcccg caacaaagtg gcgaattgtt gattgaagag gaaaaattct tcgagtttga
     2461 cacttccctg ggtggagaat gaaaatatcc cgccaaggtt ggcgccgggg agatcaatgg
     2521 caaaaacacg aaatccttcg cgttccaacc gcagctttag ttcttcagcg ccatcagctt
     2581 cgacgacttt ggtgatcaat tcgcccgcag gcgttccgag acgacaggta aactcaggca
     2641 tagatgatac tgatgagcga tgactgctga gtgacagaaa catcacctga ttgaatacca
     2701 gatgggtgtt tcgtcatttc tcattcatcc cgcatacttg aaatagaatg gtccgtacgc
     2761 tcgccatatg aattcttcgt cactggcaag agattccacc agaacgaatt ggcgaagaac
     2821 caggcacaaa actgtgcgca cttctgaaag actgatgtcg aaaggtgcgc gctcgatagc
     2881 gtcaggatgg tgattggagc gtaattgtgc gtgttgcacg tcagatatct gggaaggttc
     2941 ccaaaagaca agtatcttat ggacctgctt tcaaatcaag ggagttagcc cctcaccaga
     3001 agggatttgg caggtgtttt cggcaggaga tttggcatga gcacacagcg tttgagcaac
     3061 cccactcccg tatctgggtg gaaagaaaca acggcacgat ggtttaagcg ggcaggaatc
     3121 gcaatgcttg gcgctgcggg gagttatatg ctgctttcag ccgccgctgc ttacccgctg
     3181 tcatctctgc tggtaacgcc acaaaaaaag cggttagcgc agttgaccag ccggcatttg
     3241 cgatattttc tccggcgcaa acgcgttcat tatcaggagg ttgactttca ctcatttgat
     3301 ggaacgcgtc tgcacgggtg gtttcttcaa gctggacgct ggaagccaac cgtgattgcc
     3361 ttgcacggcg tcaccgggaa ccgaacgagt atgctccggt tcgccatggt tttgtatcag
     3421 gcagggttca acgtttttgt gtttgacggg cgaggacacg gcctgagtca gggggagttt
     3481 gtcacctatg gctatcatga aactcgtgat atctcagcct tgattgacta tctcatccgg
     3541 gaacacaagg ttcgtgaaaa atcaattggg ttgattggga tgtcaatggg cgctgcgatt
     3601 gccttgcagg ttgcggctcg tgacaaacgg atccaggcag tttgggcaga gagtccattt
     3661 tcatcgctgg aacaaatttc ggctgaatat atcgccgatg cgacacgatt gccccaatcc
     3721 atgctcaaac ccgtgacgtg gggggctatg tttgtcgcca accgacgtgg caggttcaat
     3781 gtcagtgaag tgagcccggt ggcgattgct ccacacatta cctgtccggt ccagctcgtt
     3841 catggtgctg ccgatatgtt tgtccgccca gagcattcac aacggatttt taccgcactc
     3901 acaacgcttc caaaagaatt atggttggtt ccgcgggcgg cacatacgca atgttttcga
     3961 tatgcccgcc aggaatatcg tgagcggtta gagtggtttt tcaggaaaaa cctggggcga
     4021 tagaaattgg gctgaagaag ccgggctgaa aagagaacgg gctgaagaca ttgggctgaa
     4081 gaagtcgggc tgaagacatt gggctgaaga aagcgggctt ggggtattaa acccgttttt
     4141 ttcagcccat tttcttcagc cccaagccc
//
LOCUS       DAOQDO010000240         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14770_length_4164_cov_3.249452, whole genome shotgun sequence.
ACCESSION   DAOQDO010000240 DAOQDO010000000
VERSION     DAOQDO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14770_length_4164_cov_3.249452"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..431)
                     /locus_tag="PKZ53_05135"
     CDS             complement(27..431)
                     /locus_tag="PKZ53_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39851.1"
                     /translation="MASATGYTAPPFVEKGRILRLLVWIASVLAMMVWVWFAAPASLA
                     AQLPESRRQTQKSDRKHPVPSSEDWSSLENTSERLRAPDAPDEAERFYLKKRVPSGAL
                     FPVEKLMTGKRQIASLPLFSSRLGRSLSVRAD"
     gene            complement(391..1419)
                     /locus_tag="PKZ53_05140"
     CDS             complement(391..1419)
                     /locus_tag="PKZ53_05140"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNJ39852.1"
                     /translation="MKHTECWKINPADPEPDLLAAAGQALRDGKLVAFPTETVYGLGA
                     NALDHQAVAGIFEAKGRPATNPLIVHLAEVEQVHTIASEWSTVAAQLAARFWPGPLTL
                     IVPKLEVVPDLVTAGGPTVGIRIPAHPVARALIAAAQVPVAAPSANRFTQVSPTTAQH
                     VLKGLSGRIDLVLDGGPTHVGIESTVLDVSVSPPVLWRSGMLSQAELEQHIGPIVLPY
                     QQHTPATVLPSPGQHRKHYAPKADVHLVPYADRDALRHTLERFLPGLSGDAACVGYIA
                     FEPAPIEHPAVIVNVMPARPRSFARALYAVFHAMEDAGVAHLIIEDVPPESAWNGVRD
                     RLHRAAVR"
     gene            1534..2532
                     /locus_tag="PKZ53_05145"
     CDS             1534..2532
                     /locus_tag="PKZ53_05145"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNJ39853.1"
                     /translation="MLALRFEHNQLALRDIPLPVVEDRALIRLTRAGICATDLAITRG
                     YASFSGTIGHEFVGIVEQSPDPEWVGKRVVGNINIGCGNCRECHRGDSRHCALRQVLG
                     IRSYDGAFAEYLALPIRNLHLVPDALPDSIALFAEPLAAACRILEQLVLQPDQSIVVL
                     GDGKLGQLIARMLKANGFNPLLIGKYESKRTLAEQVGIQTRTLNEKTVDVDLRQTIDV
                     VIEATGNSQAFAQALELVSPCGTIVLKSTLQGNWALKAENFVVPEITVVGSRCGRLEH
                     SLALLESGKIEVASLISAEFPLAQALDAFRVAAQPGMMKVQLLGSESEPVTGDTEG"
     gene            complement(2673..3518)
                     /locus_tag="PKZ53_05150"
     CDS             complement(2673..3518)
                     /locus_tag="PKZ53_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ39854.1"
                     /translation="MKHELSTFEFEVVVLDAEGNPIEQGTGISRQFVERISSTASLDM
                     VEIPGGSFLMGSPESEGDIDERPVHLVSIPSFFMGKYLITQVVWRTVAGLPKVNRDLI
                     PSPSHFEGELLPVENINWFDAIEFCARLTQATGRTYRLPSEAEWEYACRAGTTTPFGC
                     GETLISDLANFDASCPYKAEPQGIYRARTVPVGVFGVANEFGLYDMHGNVWEWCQDVW
                     HDTYEDAPTDGRAWLEGGKQELRVLRGGSWDYAGYGCRCAFRDRGNPKIASPFNGLRV
                     VASME"
     gene            complement(3561..4082)
                     /locus_tag="PKZ53_05155"
     CDS             complement(3561..4082)
                     /locus_tag="PKZ53_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39855.1"
                     /translation="MTNEEFLAAIKVVFESTLEEKLEQKLEAKLEQKLEPIRSDLRRL
                     DSKIDSVRDELIERIDSTKQELNERIDGVRVELVERIDSTKQELNERIDEVHTDLSDQ
                     IGETRKDLKADNQLVFAAVRDVNRSHKDTRRRAVKADADAATALQQTEIFEQRIELLE
                     DRVQRLEQQSSQH"
BASE COUNT          994 a         1117 c         1047 g         1006 t
ORIGIN      
        1 atcccagcct gagccttgag cgtgaactaa tcggcacgaa ctgacagcga acgtcccaac
       61 cgactcgaaa acaatggcaa cgatgcgatc tggcgtttcc cagtcatcaa tttttcaacc
      121 ggaaagagag cgccagacgg aactcgcttt ttgagataaa agcgctcggc ttcatctggt
      181 gcatcaggcg cccgcagccg ctcagatgta ttttccaatg aagaccagtc ttcagaagag
      241 ggtaccgggt gctttcggtc agatttctgg gtctggcgtc tggattccgg cagttgcgcc
      301 gccagactgg cgggtgcggc aaaccaaacc cacaccatca ttgcaagcac cgacgcaatc
      361 caaaccagaa gtctgagaat acgccctttt tcaacgaacg gcggcgcggt gtaaccggtc
      421 gcggacgcca ttccaggctg attcaggcgg gacatcttca atgatgaggt gggccactcc
      481 ggcatcttcc atagcgtgaa aaacggcata taaggctcgc gcaaaggatc tgggtctggc
      541 tggcatcacg ttgacaatca ccgctggatg ctcaatgggt gcgggttcaa aggcaatgta
      601 cccaacacag gctgcatcac cggataaacc gggcaaaaag cgctccaggg tgtgccgcaa
      661 ggcatcccgg tcggcatagg gaaccagatg aacatcagct tttggggcgt agtgttttcg
      721 gtgttgtcca ggtgatggca acacggttgc aggcgtgtgc tgctggtaag gcagcacaat
      781 cggtccaata tgttgctcta actcagcttg tgaaagcatc ccagagcgcc aaaggactgg
      841 cggcgacaca ctgacatcga gcacggttga ttcaattcca acgtgggtgg gcccaccatc
      901 gagcaccaga tcaatacgcc cggaaagtcc tttgagaacg tgctgggccg tggttggtga
      961 aacctgggta aaccgattgg cgcttggtgc cgccactggc acctgggcag cggcaatcaa
     1021 tgcccgtgcg accggatggg ccggaatccg aataccgacg gttggccctc cagccgttac
     1081 cagatcaggg acaacctcca atttggggac aatcaaggtg agtggccccg gccaaaatct
     1141 tgcagcaagt tgggccgcaa cggttgacca ttcgctagca attgtgtgaa cctgttcaac
     1201 ctcagctaaa tgcacaatca acggattggt tgccggacga cctttggcct caaaaatgcc
     1261 ggcaactgct tgatgatcaa gggcatttgc acccagccca tatacagtct cagttgggaa
     1321 agcgaccagt ttcccatccc gtaatgcctg accagccgct gccagcaaat cgggttctgg
     1381 atctgctgga ttgattttcc agcattcagt gtgtttcaca attgttgaac tcccattcag
     1441 aaatatagcg tgtgatctga gaaaaacgcg cctttatgac tttcccggct tacaatcttg
     1501 cctttcgttc tttcacgacg tcactgactt accatgcttg cacttcgttt tgaacacaat
     1561 caacttgccc tgcgcgatat ccccttacct gtggttgaag atagggccct gattcggttg
     1621 acccgcgccg gtatttgcgc cacagacctg gcgattactc gcggctatgc cagcttttcc
     1681 gggacgattg gccatgaatt tgtcggtatc gtcgagcagt caccagatcc agagtgggtt
     1741 ggcaaacgtg tggttgggaa tatcaatatt gggtgtggca attgccgcga gtgccatcgt
     1801 ggcgacagcc gacactgtgc cctgcgccag gtgctgggaa ttcgatctta tgatggtgcc
     1861 tttgctgaat atctggccct gccaatcaga aatctgcacc ttgttccaga tgccctgccc
     1921 gattcaattg ccctttttgc cgaaccgctg gcagccgcct gtcgaatcct ggaacaactt
     1981 gtcttgcaac cagatcaatc gattgtggtt ttaggagatg gaaaacttgg tcagttgatt
     2041 gcccggatgc tcaaagccaa tgggttcaat ccattgctca ttggtaaata tgagagcaaa
     2101 cgcaccctgg ctgaacaggt gggaattcaa actcggacct tgaacgaaaa aactgttgat
     2161 gttgacttga gacaaacaat tgatgtggtg attgaagcca cgggcaactc acaggcgttt
     2221 gcccaggcat tagaactggt ttctccctgt ggcactatcg tcctgaaatc aacacttcag
     2281 ggaaactggg cactcaaagc agaaaatttt gtggtgcccg aaatcaccgt ggttgggtca
     2341 cggtgtggac ggctggaaca ttcgctggcg ctgctggaat caggaaaaat cgaagtggca
     2401 tcactcatca gtgccgaatt tccactggct caagcccttg atgcctttcg cgttgccgcc
     2461 caacctggaa tgatgaaagt tcagttgctc ggctctgaat cggagccggt gactggtgat
     2521 actgaaggct gaagactttc gggcttgggg ctgagggcct ggggctgagg gctgaagaac
     2581 tggttttctt tcatccctca tccttcatcc ctcatccctg tgcttgcccc aggccctcag
     2641 ccctgtgtct tcaaccccgg ttttttcagc cttcactcca tactggcaac gacccgcagg
     2701 ccattaaatg ggctggcaat ttttgggttg cctcgatccc ggaacgcaca ccggcagcca
     2761 tagccggcat aatcccacga accaccacgc aagactcgta actcctgttt gccaccttca
     2821 agccacgcac ggccatcagt tggagcatct tcataggtat cgtgccagac atcctgacac
     2881 cattcccaaa cattgccgtg catatcgtag agtccaaact cattggccac gccaaagaca
     2941 ccaaccggaa cggttcgggc acgataaatc ccctgcggtt cagctttgta tgggcaactg
     3001 gcatcaaaat tggccagatc ggaaatgagc gtttctccac aaccaaacgg cgtcgtggta
     3061 cccgcacgac aggcatattc ccattcggct tcactgggca ggcgataggt gcgacctgtg
     3121 gcctgtgtga gccgggcaca aaattctata gcatcaaacc agttaatgtt ttcaaccggg
     3181 agcaactcgc cttcgaaatg cgacggggat ggaataaggt cgcgattgac ttttggtaac
     3241 ccggcgactg tccgccaaac cacctgggta atcagatatt tccccataaa aaatgagggg
     3301 atggagacca gatggacagg ccgctcgtca atatcacctt cagattccgg tgaccccatc
     3361 agaaacgagc cgcctggaat ttcaaccata tccagcgaag cggtactcga aattcgttcg
     3421 acaaactggc gtgaaattcc tgttccctgt tcaattggat tgccttcagc gtccaacacc
     3481 accacttcaa actcaaaagt tgataattcg tgtttcaaat ccaggtcctc tgcaacagac
     3541 accccaggtc agtgcacggt ttaatgttgt gaagattgtt gttccagccg ttgaacacgg
     3601 tcttcgagca attcgatacg ttgctcaaag atctcagttt gttgaagtgc agttgccgca
     3661 tcagcatcag ctttcacagc acggcgtcgg gtgtctttat gtgaccggtt gacgtccctg
     3721 acagcagcaa aaacaagctg gttatcagcc ttcaggtctt ttcgggtttc accgatctga
     3781 tcagataagt cagtgtgaac ctcatcaatg cgttcattga gttcctgttt ggttgaatca
     3841 atgcgctcga ccagttccac ccgcactcca tcaatgcgtt cattgagttc ctgtttggtt
     3901 gaatcaatgc gctcaatcaa ttcatcacga accgaatcaa tctttgagtc caatcgtcgc
     3961 agatcagacc gaatcggctc taatttttgc tcaagctttg cttccagctt ttgctcaagc
     4021 ttttcttcca atgtggattc aaaaacaact ttgattgcgg ccaggaattc ttcattcgtc
     4081 atagtattca aaacctcaat tcaaggaatt ttcaaagaat acacggttgc cttactttcc
     4141 agaagccggt gtttcttttt gcgg
//
LOCUS       DAOQDO010000241         4159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14806_length_4159_cov_2.660331, whole genome shotgun sequence.
ACCESSION   DAOQDO010000241 DAOQDO010000000
VERSION     DAOQDO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14806_length_4159_cov_2.660331"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1019
                     /locus_tag="PKZ53_05160"
     CDS             204..1019
                     /locus_tag="PKZ53_05160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ39856.1"
                     /translation="MDLYRNQTTLITGASSGIGEAFAHELARRGSHLILVARTESKLQ
                     KMAAELSKKYSVRCEVIPADLIQAGAAKMVYQETQRRGLTVDFLINNAGFGTYGPFDT
                     LDPHRDHDEVMLNVTSVVDLTHQFLPDMVRRKSGGIINVASTAAFQPLPYMAVYGATK
                     AFVVSFSEALWAEYRNQGVRVMALCPGATETPFFDVVGASEPAVGIKETPEKVVRVGL
                     KAFEQGRCFVVSGTANYLLSLTSRFTPRSLTALIAQQFLKPKRQATKTASARS"
     gene            complement(1151..2557)
                     /locus_tag="PKZ53_05165"
     CDS             complement(1151..2557)
                     /locus_tag="PKZ53_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNJ39857.1"
                     /translation="MDQLIFASATELAQAIRAKDVSSEEVVSAHLHRIQTINPKLNAF
                     TEVFAEQALTQARQADQSLAQGTLLGPLHGVPISVKDVFDIAGVRTVTGSRLRETTVA
                     TTDAPVVAAVKRAGAVILGKTNVPECGLDYRSENLVFGRTSNPWDLSRVPGGSSGGEA
                     AAIASGCSPIGLGSDLGGSIRVPSHFCGIAGLNPTPGRISSLGHFPTVLGPMSLGFSA
                     GPMARRIDDLSLLFQIMVEADPRDPATVPLSARTGDTPVNGGLKVMWYASDGVTPVTA
                     ETAASVEQAAALLGEQGFETVHHKPAGVERGFELWFYFLGQSSVPLLLNMYKGKEDLM
                     GPLMQGLLHIIQPMSFEQFLTVWIERDLLRRSVLNELGDNGVLLCPVAATPAFSHDQQ
                     DKFEINQHKVDYLQAFTYAQIYNLLGLPSVVVRCGKSPEGLPIGVQIVGRPFEEEKVL
                     AVAKALENAGGGYQRPSL"
     gene            2792..3307
                     /locus_tag="PKZ53_05170"
     CDS             2792..3307
                     /locus_tag="PKZ53_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ39858.1"
                     /translation="MTEILISRFRRWFEYEQDSHARVLASLETVPAASRSAEAFQQAL
                     DLMGHMVFARRLWLYRLGGLAEKPVVFPKTVTLDELAAQFETMHTAWSDYLNSVTDDD
                     LVKIFEYQSLDGKRFRNTVEDILAQLFGHSWYHRGQIASRIKAIGGTPAVTDLVFWSR
                     ELIEEPDTLGG"
     gene            3311..3937
                     /locus_tag="PKZ53_05175"
     CDS             3311..3937
                     /locus_tag="PKZ53_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39859.1"
                     /translation="MSSSPKHQLTEAEYLAIERRAEFRSEFYRGEMFAMAGASRRHNR
                     IVTNLVARLDQQLRDRPCNVYSNDMRILIPNAGLYTYPDIVITCGAEQFLDDTEDTLL
                     NPLVIIEVLSDSTEAYDRGRKFEQYQKIASLQTYVLVAQNAQRVEQYARQADSKFWTY
                     SESHEAGEVITIAAINCELMLEEVYSKVEPTTETQVGDTLPENAGGVS"
BASE COUNT         1033 a         1068 c         1089 g          969 t
ORIGIN      
        1 atcgggctaa gggctgaggg aaatacaata tttccaatat tttagctttt ccaattgact
       61 ccaaatctga caagatgacc ggctgacagg gtgacatggt gaattttttc atcgctcatt
      121 ccttctgaaa accctgaacc ccgaacccta aaataaaatg gaatgaacat tcattcccaa
      181 ttcatcatcg aaaggaactc atcatggacc tctatcgcaa tcaaacaacc ctgatcactg
      241 gcgcttcctc tggcatcgga gaagcctttg cccacgaact ggcccgccgt ggctcacatc
      301 tgattctggt cgcccgcacc gaaagcaaat tacagaagat ggccgccgaa ctctccaaaa
      361 agtatagtgt gcgctgtgaa gtgattccgg cggatttgat ccaggccggc gcggctaaaa
      421 tggtgtatca ggaaacccaa agacgcgggc tgacggtgga ttttttgatc aataatgccg
      481 ggtttggcac gtacgggcca tttgacacgc ttgatccaca ccgcgaccac gacgaagtca
      541 tgctcaatgt gacctctgtt gtggacctga cccatcagtt tttgccagac atggtgcgtc
      601 gtaaatcagg cggcatcatc aacgttgctt cgaccgctgc ctttcagcca ttgccctaca
      661 tggcggtcta tggcgccacc aaagcgtttg ttgtgtcgtt tagcgaagcg ctctgggccg
      721 aatatcgaaa tcaaggcgtc agagtcatgg ccctgtgtcc tggcgcaacg gaaacgccgt
      781 tttttgatgt cgtgggcgcc tctgaacctg ctgtcggaat caaagaaaca cccgagaaag
      841 tggtcagagt cggtctaaaa gcatttgaac agggccgatg ttttgtggtt tccggaacgg
      901 ccaattacct gctgtcgctc acctccaggt ttacgccacg ctcgttgacc gctttgattg
      961 cccagcagtt tttgaaacca aaacgacaag cgaccaaaac cgcttcggcc cggtcataag
     1021 catttcggaa tcagaaacca cggaagacac gggaaaaatc catcaccagc cagtaaagca
     1081 ttggatttta ttttcgtgtg tttcggtgtg ttccgtggtt gatacactgc taaagttgag
     1141 aacactgggt ttacagcgag ggacgttgat agcctccgcc agcgttttcg agcgccttgg
     1201 cgacggccag caccttttct tcttcaaatg gtcttccaac gatctgaact ccaatcggta
     1261 gcccttccgg tgatttgccg caccggacaa caacgctcgg aagtccaagc aaattataga
     1321 tttgggcgta ggtaaatgcc tgcagatagt caaccttgtg ctggttgatt tcaaatttgt
     1381 cctgctggtc gtgggaaaaa gccggggttg ccgcgaccgg acacaacaac accccattgt
     1441 cacctagctc attgaggacc gaccttcgca gcaaatcgcg ttcgatccag acggtcagga
     1501 attgctcaaa cgacatcggc tgaatgatgt gcagtaaccc ttgcatcaaa ggccccatca
     1561 ggtcttcttt gcctttgtac atgttgagca ggagcggcac ggatgactgg ccaaggaaat
     1621 agaaccacaa ttcaaagccg cgctcgacac cagccggttt atgatgaact gtctcaaagc
     1681 cctgttcacc aagcaacgcg gctgcctgtt caaccgaagc cgctgtttca gccgtgactg
     1741 gggtgacacc atcagatgca taccacatca ctttcaagcc accattcacc ggagtatcac
     1801 ctgtgcgagc cgaaagagga accgttgccg ggtcgcgtgg atcggcttca accataatct
     1861 gaaacagcaa ggagagatca tcaattcgac gcgccatggg gccggcagaa aacccgagcg
     1921 acatcggccc cagaacggtt gggaaatggc caaggcttga aattcgaccc ggtgttggat
     1981 tgagtccagc gatgccgcaa aaatgagatg ggacgcgaat tgaacctcct aaatctgagc
     2041 cgagcccaat cggggaacat ccagaagcaa tcgccgctgc ttcgccgccg ctggagcccc
     2101 caggaacacg gctcagatcc cacggattgc tggtccggcc aaaaaccaga ttttcgctcc
     2161 ggtaatcaag tccacattcg gggacgttgg tttttcccaa aataactgcg ccggcgcgtt
     2221 tgacggcagc cacaacgggg gcatcagtcg tggcaacagt cgtttcgcgc aatctggagc
     2281 cggtaaccgt ccggactcca gctatatcga acacatcttt gacggaaatg ggcacgccat
     2341 gcagcggtcc cagaagagtg ccctgtgcca ggctctggtc agcctgccga gcctgggtaa
     2401 gcgcctgttc ggcaaatact tcagtaaagg cgttgagttt ggggttgatg gtttgaatgc
     2461 ggtgcaggtg ggcgctgaca acttcttcgg aagaaacatc tttggcccgg atggcctgtg
     2521 ccagttccgt ggctgacgca aaaataagct ggtccatggg aaagctcctg gtgaatttgg
     2581 atggtatgct tgcgaaccga acacgggaaa agggagaaaa aaacgagaat tttcttgggt
     2641 gattgacgca gcttatacag ggttacaggc gcctttggat gacacctcag ccagtttttg
     2701 tgaggtctga ttgggttgcc ctggcccagg gaaactatgg tagattcagc caccggacaa
     2761 ttctcaaatc ccgatcttcc aaattgaata tatgactgaa atcttgattt cccgcttccg
     2821 tcgctggttt gagtatgaac aggacagcca cgcccgcgtg ctggcttcgc ttgaaactgt
     2881 tccagcagca tcgcgctctg ccgaagcatt tcaacaggcg ctggacctga tggggcacat
     2941 ggttttcgcc cggagactgt ggctctatcg gctgggggga cttgccgaaa agcccgtagt
     3001 tttccccaaa actgtgacgc ttgacgaact tgctgcccag ttcgaaacca tgcacacagc
     3061 gtggtccgac tatctgaact cggtgaccga tgacgacctg gtgaaaatct ttgaatatca
     3121 gagtttggat ggcaaacgct ttcggaacac ggtcgaagac atcctggcgc agctttttgg
     3181 gcattcgtgg tatcaccggg gccagattgc ctcgcggatc aaagcgattg gcggcacgcc
     3241 ggcggtgacg gatctggtct tctggtcacg cgaattgatt gaagaacctg acaccctcgg
     3301 aggttgaacg atgtcatcaa gccccaaaca ccaactgacc gaagccgaat atcttgccat
     3361 tgagcgtcgg gctgagttca gaagtgaatt ttatcgaggc gagatgtttg cgatggcagg
     3421 tgccagccgc cgtcataacc gaattgtaac caatctggtg gccaggcttg atcaacagtt
     3481 gcgggatcgg ccctgcaacg tgtattccaa cgacatgcgg atattgattc cgaatgcggg
     3541 actctatacc tatccagaca ttgtgatcac gtgtggagca gagcagtttc ttgacgacac
     3601 agaagacacc ttgctcaacc cgctggtgat tatcgaagtc ctctctgatt caaccgaagc
     3661 ctatgatcgc gggagaaagt ttgaacagta ccaaaagatt gcttcgttac agacctacgt
     3721 gctggtcgcc caaaatgctc aacgcgtcga acagtacgcc cggcaggctg acagcaaatt
     3781 ttggacgtac tccgaaagcc acgaagccgg cgaagtcatc accatcgcgg ccatcaactg
     3841 tgaactgatg cttgaggagg tttattcgaa ggttgaacca acgaccgaaa cgcaggtcgg
     3901 cgatactttg ccagagaatg caggtggtgt tagttagcgg taaaacagca ataagtctaa
     3961 atttttcaat tagtaagagg ctttgtattc tggtcaaggt agttaatggc agagctaaag
     4021 tgaatgatca agttacgagt tccgtcttca ggcggtttgg tcctgcactc gtcaggtcct
     4081 ttttgtgttg cttaacctgt gtttattgtt accctttcca cgaaatagca taaacggatt
     4141 agaaaaatat aattggttt
//
LOCUS       DAOQDO010000242         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14824_length_4156_cov_3.744209, whole genome shotgun sequence.
ACCESSION   DAOQDO010000242 DAOQDO010000000
VERSION     DAOQDO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14824_length_4156_cov_3.744209"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKZ53_05180"
     CDS             <1..276
                     /locus_tag="PKZ53_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39860.1"
                     /translation="AAKEEARALAPAPDESEEVTTERPRRGRRPKAVNIEITTPRTVP
                     IEVEEVKVEAKPRRGRRPKAIPAEVIATEAQPASVTSIRTRRQRRTS"
     gene            483..1310
                     /locus_tag="PKZ53_05185"
     CDS             483..1310
                     /locus_tag="PKZ53_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ39861.1"
                     /translation="MANKTGSSEPDVHGPANSTSRRKYAYKPPSPLLTALEIRASWEL
                     GAWLMSLPLLKSAPKGDGHPVLVFPGLAASDFSTIPLRQFLKGIGYTPYPWNFGRNLG
                     PRPGVLRGCAEHVKELREKHGQNVSLIGWSLGGLYAREVAKIAPEVTRCVITLGTPFT
                     GHPKANHAWRFFEFASGLKVGDPKLHEPLRHSPPVPTTSIYSRSDGVVSWKCSLETES
                     PITENIEVIASHLGMGVNPATLFAIADRLSQPEGQWKKFERYGIRKLFYPEPRYYRH"
     gene            2101..2358
                     /locus_tag="PKZ53_05190"
     CDS             2101..2358
                     /locus_tag="PKZ53_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39862.1"
                     /translation="MNITTPIEGIDRFFSLAKKNYPKAFADDAENVWQLVEAFPISGC
                     NPAYECDLVLQEYRSTERRSYTYWSCKIDISELSTTPERRH"
     gene            2833..>4156
                     /locus_tag="PKZ53_05195"
     CDS             2833..>4156
                     /locus_tag="PKZ53_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ39863.1"
                     /translation="MTESSSFQSQINSLLANQVEYPAPLSVRHFVHRPVTDSDYQYSI
                     THTEQFWADEATKFIWTRPYDQVFQTDGVHHQWFVGGKTNITLNALDRHADSAHCNRV
                     AFIWLGEDGSERIVTYNQLRRMVGRLANGLKSIGVQKGDRVIIYLPLTIEGIASMLAC
                     ARIGAIHSVVYAGLGHTALRDRIDDAQAKVVIGGDVGYRRGRAVPLKSIVDEAVADLE
                     CVEKVVFFSRRDPAAEITSVRQMDFNDLLKFPADCPAEEMDAEDPLFILYTSGTTGKP
                     KGVVHVHGGFMVGTTYHLETFLDVSPRDVFWCTSDIGWIVGHSYIVYATLCTGATTLI
                     REGSIDYPHPGVAWEIVERYGVTKMFTAPTALRMYMKFGEALPAKYDISTLRVIACAG
                     EPLNPEAWRWAQTHLAGDGKWGYVVDNWWQTELGGPTIGTAPNIAMRPG"
BASE COUNT         1049 a         1073 c          997 g         1037 t
ORIGIN      
        1 gccgccaaag aagaagcccg tgcccttgcc ccagcaccgg atgaatcaga agaagtcaca
       61 actgagcgcc cacgacgcgg aagacgacca aaagccgtca acatcgaaat caccaccccg
      121 agaacggtcc ccatcgaagt cgaggaggtg aaagtcgaag caaaacccag gcgtggtcgc
      181 cgcccaaaag caattccagc cgaggtcatc gcgactgaag ctcaaccagc atccgttacc
      241 agcatccgaa cccgcagaca acgccgcaca agttgaatga ggaattgaga atgaagaatg
      301 aagaatgaag aattgagaat tgagaattga tcgagagcct ggccatcagg attgaatact
      361 ttcgaaaaag catcaggtac cggggacgaa tcaaattctt cactctcaat tcttcattcc
      421 tcattctcaa ttcttcattc ttcattctca attcttcatt cctcataagg acttgatcgt
      481 gtatggcaaa caagacaggc tcatccgaac cagacgtcca tggaccagca aacagtactt
      541 cacggcgaaa atatgcgtac aaaccaccct caccactcct gactgcttta gaaatccgcg
      601 cctcgtggga acttggcgcc tggttgatgt cactgccgct tctcaagagc gcgcctaaag
      661 gtgacggtca cccggtgctg gtctttcccg gccttgctgc cagtgatttt tcgaccatcc
      721 cgttacgtca gtttttgaaa ggaatcggat acacaccgta tccctggaat tttggccgca
      781 atcttggccc tcggcccggc gttctgcgcg gctgtgccga acacgtgaaa gaattgcgcg
      841 aaaaacacgg ccagaatgtc agtttgattg gctggagcct gggcggcctg tatgcgcgtg
      901 aagtggccaa gattgcccct gaagtcaccc ggtgtgtcat cacgctcgga acgccgttta
      961 cgggtcatcc gaaagccaat cacgcgtggc ggttttttga attcgccagc gggttaaaag
     1021 tcggagaccc aaaactgcac gagccgctca gacattcccc gccggttccg acgacttcga
     1081 tctatagccg ctcagacggg gtcgtttcct ggaaatgctc cctcgaaaca gagtctccca
     1141 tcactgaaaa tatcgaagtc atcgccagtc atctcgggat gggggtcaat ccagcgacgc
     1201 tgtttgccat tgctgaccgg ctctctcaac cggaaggaca atggaaaaag tttgaacggt
     1261 acggcattcg caaattgttc taccctgaac cgcgatacta ccgacactaa cggcagccag
     1321 ggcgcaagga gcgagtcgtc aatattagtg aaatttgtga acgcgttcac aaccagttaa
     1381 aaagtagaaa acaaatgctt taagtggtcc tggccggttt tttcactttt gttttaagta
     1441 ccttgtcaaa agtttcctga gatattgaat tttggaagtg tttgatttct ttccgtggtt
     1501 tccgtggtta gatgtctgga aattttcggt aaggtactta ccactcgctc ctccttcctc
     1561 accactcgct ctggttggcc actcacgttg aactttcaat ttccaggttt attacaaaag
     1621 aacaccagga atcagccgga ttgacgatag ggaggccatt gctttctgac gagacagggc
     1681 agaattggat tgatcctcgg aatgaatcaa aaaatagata tactcttcat acaacttata
     1741 gttataaccg aagtatgagg gaatggtgcc gggccatcgg gttgagacgg gaattgatga
     1801 cccgcacttc caaattcttt cctttttttc agaccagaac tgactttttc tctgtaatca
     1861 tgggattcag gtctatccag tttcttcaag ttgtgagaaa ctagattgct tcacacgaac
     1921 tatggtttgc ttcccatcga ctcagcaaaa catcggacaa cttgaggcaa cctcctggtt
     1981 acatggtttt aatctgagag tgaaatactg gctcatcttt gtgcagttca ttacaccctc
     2041 gaattgcgtc ccttgctggg gattataaca acaacaagag aaaggaagag aattatggat
     2101 atgaatataa ctactccgat tgaaggcatt gatcgctttt tttcactggc gaagaaaaat
     2161 tatccaaagg catttgctga cgacgctgag aatgtttggc aactggtgga agcatttccc
     2221 atctctgggt gcaatccggc gtatgagtgt gacctggtgt tgcaggagta tcggtctacg
     2281 gagcgccggt cctatactta ctggtcatgc aaaattgata tttcagagct ttcaacgact
     2341 cctgaacgac ggcactgaat catacaggct gaacctggag gtcacgcccg atgccgcgtg
     2401 actccgggtt catcagaatt tttcccctaa caacctgcct caacgaattt ctccccactg
     2461 ctgaaccgta aaactcccca tttttacttt cgctgctttg tattgaaatg actgggaact
     2521 gcctttcaat ccggcctttt tattcccata gttgacaaac catttatttt caatatcttt
     2581 cctgattcat cattcctggt cgtcaacagt cagtttgaat ttcctggtca gggaacacaa
     2641 attgcacaag gatgaaactg tgccgccaga ttccgtttca cccattcctt gtccgttact
     2701 ctttcgcttg aacccagaga ttacaccatt ttgcaatgaa gtcctccgat tagaagacag
     2761 ctaaatcatt ggaaaaattg tatttccctg aaccctgaac cctgaaccct gaaccctaat
     2821 tggtattccc ctatgactga atcatccagc ttccaatccc aaatcaactc tcttcttgcc
     2881 aatcaagtcg aatatccagc cccgctctcc gtcaggcact ttgttcatcg gccagtgact
     2941 gattctgact accagtattc gatcacacac accgaacaat tctgggcaga cgaagcgaca
     3001 aaatttatct ggaccaggcc atatgaccag gtttttcaaa ctgatggggt ccatcaccag
     3061 tggtttgtcg gtggcaaaac caatatcacg ctcaatgccc tggaccggca cgctgattcg
     3121 gcccactgca accgggtcgc gtttatctgg ctgggtgagg acggcagtga gcgcatagtg
     3181 acctataacc agcttcgtcg gatggttggc cgattggcaa acgggttgaa atccattggg
     3241 gttcagaaag gcgaccgggt catcatttat cttccactaa ccattgaagg aattgcctcg
     3301 atgctggcct gtgcccggat tggggcaatt cattcggtgg tctatgccgg gttaggtcac
     3361 acggcgcttc gggatcggat tgatgacgcc caggccaaag tagtgattgg gggcgatgtg
     3421 ggctaccgcc ggggaagagc cgttccactc aaatccattg ttgacgaagc cgtggctgac
     3481 cttgaatgcg tcgaaaaagt cgtctttttt tcgcgaaggg atccagccgc tgaaattacc
     3541 agtgtccgcc agatggattt taatgacttg ctgaaatttc cggcggattg cccggcggaa
     3601 gaaatggacg ccgaagaccc actctttatt ctttacacct cgggaacgac cgggaaaccc
     3661 aaaggggtgg ttcacgtgca cggcgggttt atggttggaa cgacctatca cctggagacc
     3721 ttcctcgatg tgagtccacg cgatgtcttc tggtgcacgt cagatattgg ctggattgtc
     3781 gggcattctt atattgtcta tgccacgctc tgtactggtg ccacaacctt gatccgggaa
     3841 ggttcgattg attatccgca tccgggcgtg gcctgggaaa tcgtcgagcg gtacggcgtt
     3901 acaaagatgt tcaccgcccc aacagcactc cgaatgtata tgaaatttgg cgaagctctg
     3961 ccggccaaat atgacatttc gactctgcgc gtcattgcct gtgccggaga accactcaac
     4021 ccggaagcct ggcgctgggc gcaaacccat ctcgccggag acggcaaatg gggctacgtg
     4081 gtggataact ggtggcaaac tgaactcggc ggaccaacaa ttggaacggc acccaatatt
     4141 gccatgcgac ctggaa
//
LOCUS       DAOQDO010000243         4151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14848_length_4151_cov_3.148193, whole genome shotgun sequence.
ACCESSION   DAOQDO010000243 DAOQDO010000000
VERSION     DAOQDO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14848_length_4151_cov_3.148193"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..3564)
                     /gene="metH"
                     /locus_tag="PKZ53_05200"
                     /pseudo
     CDS             complement(1..3564)
                     /gene="metH"
                     /locus_tag="PKZ53_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481837.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            complement(3577..>4151)
                     /locus_tag="PKZ53_05205"
     CDS             complement(3577..>4151)
                     /locus_tag="PKZ53_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ39864.1"
                     /translation="LISTSPLVKTYTLNEFWELPEPEDHSRRELIAGVLYMARPPNYS
                     HDSITSRLIDALVAHRFSIADKGKLYTPRAAIWTSDKTYLEPDLFYLSAELEASLDKD
                     FRSTADLVIEILSPASEQYDRTTKADTYGVLGVRELWLVDPTNCTIEVRTQTGNGFDK
                     GTVFSQGEVIKSQIFPQLDLPVTLIFADLG"
BASE COUNT          997 a         1107 c         1008 g         1039 t
ORIGIN      
        1 cggatgggca aaatacagac cactgaccga actggccgga tacatggcaa aactctcggt
       61 taaggtaatg ccggtgtttt tctcaacctg gagcaggtca aagagactcc gtttttcagt
      121 gtggtccggt tgggccggat agcctggtgc cggtcgaatg ccccgatatt tctctttgat
      181 caattcttcc gggctcaggt tttcagtctg accatacccc cacgcctggc gggcctgctg
      241 atgtaaattc tcggcaaaag cttcggccag tcggtcagaa agggctttga ccaggatcga
      301 actgtaatca tcatgatcgg cttcgtactg ccggcagagt tcgtccatac caatgcctgc
      361 cgtgacagca aatccgccaa tatagtcagc ccggccagat tcttttggcg cgacaaaatc
      421 agccagcgcc agttgagatt gacccgacga tttttcggat tgctgccgca gggtataaaa
      481 acgggtcagt acttcggttc gggcgtcatc agcatagacc tcgatgtcat ctccgacgct
      541 gttggccgga aataatccaa aaacgccgtg cgcggtgatt tgctggtcag aaatgatttt
      601 ttcaagcatt ttctgagcat cggcgaaaag ctcacgggct ttatcaccaa tgactggatc
      661 atcgaaaatc tttggatacc gcccgcgaag ttcccaggca tggaaaaacg gtgaccagtc
      721 aatgtagggc accagtttct caagcggata atcggtcaaa acctggattc cgatctgggt
      781 tggaacatca atttcagcct gacgccagtc aaatcgcggc ttccgcatcc gggcctgatc
      841 gagcgtcaat gacgtcgccc gtgcggtgcg gccactatgc tcctggcgaa gttgttcgag
      901 ttcagcccga ttcttttcag caaattcaat ctttaattct gaattgatca attgaccgac
      961 gaccccaacc gcccgcgaag cgtcaatgac gtggaccacg ggttgaccat aatgcgggga
     1021 aattttgacg gcagtatgaa ttttgctcgt cgtggcaccg ccaatgagca acggaacacg
     1081 aaactgctgg cgctccattt ctttggcgat gtgaatcatt tcatcaagtg acggcgtgat
     1141 gagtccgctc aacccgatca tatccacctg atgctcgcgg gcctgtttca gaattgtttc
     1201 acagggcacc atcaccccta agtcaatgac ttcatagttg ttacacccaa gcaccacccc
     1261 gacgatgttt tttccgatgt cgtgaacgtc gcctttgacc gtggccatca ataccctggc
     1321 cagtgcccga ctggcgccac tcgattgttt ttcggcttcc atataaggct gcaggacggc
     1381 caccgctttt ttcatcaccc gggcgctttt gacgacctgc ggcagaaaca tttttccagc
     1441 gccaaacaga tcgccgacga tattcatgcc atccatcaag ggaccttcaa tcacttccag
     1501 aggacgggct tacaactggt cggcttcttc gacgtcggct tcaatatgct caacaattcc
     1561 tttgatcaag gcatggctca atcgttcctg gacggtccct tttcgccagt cagcatcttt
     1621 gacctgggtt ttatcctgtt gtttgaatga ttcggcaaat gtaatcaggc gttcggttga
     1681 gtctgggcgc cggttgagca acacatcttc aaccagttcg agcaaatctt tcgggatttc
     1741 ttcatagacc gccagttggc cggcattcac aatgcccatg tcgagcccgg ccttgatggc
     1801 gtgatagaga aatgccgagt gcatggcttc acgcaccgga ttattgcctc gaaaagaaaa
     1861 cgaaatgtta ctcacaccac cgctgacttt gcagtgcggc aggttttgtt taatccagcg
     1921 cgtggcttca atgaaattga ccgcgtagtt attgtgctct tcgatgccgg tggcaacggt
     1981 caaaatattg ggatcaaaga tgatgtcttc cggtggaaac ccaactttct cggtcaggat
     2041 gcgataggct cgctcgcaaa tttcgatttt gcgttcatag gtgtcagcct gcccctgctc
     2101 atcaaaagcc atcaccacca ccgccgcccc atagcggcga acttttcggg cagattcgat
     2161 gaagggtgct tcgccttcct tcaaactgat cgagttgaca atacattttc cctgcacaca
     2221 tttcagaccg gcttcgatga ctgaccattt cgaactgtca atcatcaccg gaacctttga
     2281 aatatccggt tctgacatca ccagatgcag gaatttgacc ataatggctt cggcatcgag
     2341 catgccttca tccatattga tatcaatgat ttgggcacca ttttcaacct gctggcgggc
     2401 aattgccagc gcctcttcat attgcccctg cttaatcagt tgggcaaatt tcggcgaacc
     2461 agtcacattg gtgcgctcac cgatattgac gaagttcata tccgggcgca tcaccagcgg
     2521 ttccaacccg ctaaaccggg tcacagactg aggtgatgga ggaacacgcg gagtcactcc
     2581 tttcaccgcc gccgcaatcg cggaaatatg aagcggcgtt gttccacaac atccaccaac
     2641 aatattgagc cagccgttgg ccgcaaactc ctggatcaat tcgcccataa tctccggagt
     2701 ctgatcaaat ccaccaaaca catttggcaa tccggcattg ggataacagg tgagaaagat
     2761 cggcgccatg tcagagagtt cttcaacata cgggcgcatt tctttggccc caaacgcgca
     2821 attgatccca atactcaaca atggaaaatg cgataccgaa atccagaacg cagccagggt
     2881 ttgccccgaa agggttcgtc cactttggtc cgtaatcgtc accgaagcaa tgactggcac
     2941 ccggcgtcca gtctcttcaa atatccggtc aatggcaaag agcgcagctt tgaggtttaa
     3001 agtgtcaaac gtggtttcca cgagtaacag gtctgaaccg ccgtccatca atcctttggc
     3061 ctgttcggca taggcttcga ccagttcgtc aaaggtaact gaccggtggc ctggattgtt
     3121 tacatcacgt gacagcgacg ccgttttatt ggttggcccc atcgaacccg ccacaaaccg
     3181 tggacgtcca tcaagagcca tctgggcctc gacggcccga cgcgcaacct gggccgacgc
     3241 cacattcaag gcatagactt ctgattcgag ctgatagtca gccatcgaaa tcgaagtggc
     3301 actaaaggta ttggtttcaa taatatcggc cccagcttca agatatgccc ggtggatggc
     3361 ttcgataatg tggggttgtg taatcgagag gagatcgttg caacctttca aatcacctgg
     3421 gtgatcaaca aattgagttc cgcgataatc ggcctcttgc aacttataac tttgaatcat
     3481 cgtgcccatt gcgccatcaa taaccagaat tcgttcgcgc agcaggctcc tgagcaactc
     3541 cgtacagttt gtgtcgtgtt tcattcaatg gcggtcctac cctaaatcag caaagataag
     3601 agttactggg aggtcgagtt gagggaaaat ctgggatttg atgacctcac cctgactgaa
     3661 aaccgtgcct ttgtcaaagc catttcccgt ctgagttctg acttctatgg tgcaattagt
     3721 tgggtccacc agccacaatt cacgcacgcc cagcacaccg taggtatcag ctttggtcgt
     3781 tcgatcatat tgctcagacg ctggagataa aatttcgatc actaaatctg ccgtactgcg
     3841 gaagtcttta tctaatgatg cttccaattc ggcggacagg taaaacaaat caggttccag
     3901 gtaggttttg tcacttgtcc agattgcggc ccgtggtgtg taaagctttc ctttgtcagc
     3961 aattgaaaac cgatgtgcca ccaaagcatc aatgaggcgt gaggttattg agtcgtgcga
     4021 ataattcggt ggtcgggcca tatataacac tcctgcaatg agttcgcgcc tggagtgatc
     4081 ctccggctcg ggcagctccc aaaactcatt gagagtatag gttttgacca gtggtgaggt
     4141 tgaaatcagt a
//
LOCUS       DAOQDO010000244         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14863_length_4149_cov_2.728627, whole genome shotgun sequence.
ACCESSION   DAOQDO010000244 DAOQDO010000000
VERSION     DAOQDO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14863_length_4149_cov_2.728627"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..298
                     /locus_tag="PKZ53_05210"
     CDS             56..298
                     /locus_tag="PKZ53_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNJ39865.1"
                     /translation="MKTITATKARTNFFSLLDEAAQSHEPIQITGKRGNAILISEDDW
                     RSIQETVYLLSIPGMRESIREGLATPVEECDRELEW"
     gene            295..561
                     /locus_tag="PKZ53_05215"
     CDS             295..561
                     /locus_tag="PKZ53_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNJ39866.1"
                     /translation="MSNWTLVFTRQAQKDAKKLAQTGLRSKAEELLEILNRDPFQNPP
                     PYEKLVGDLAGAYSRRINIQHRLVYQVYQDIQTVKILRMWTHYE"
     gene            869..1495
                     /locus_tag="PKZ53_05220"
     CDS             869..1495
                     /locus_tag="PKZ53_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39867.1"
                     /translation="MDQNHRGILSLPPFLQDLLRNQPPNPFEILFGKRFQIQPRHLSR
                     AFIISVCLLAVAFYFHIFCFLAIFPQSQRFITFQGGAFAGMGGGTLYHYRTSDGENLG
                     MRTLFDGPISIKELTAVSDLQLEVSVARYISEQNVIEYERVHSPHFPKVIEKYVARNT
                     TSQLAPYSIYYIYNGYYLFIIDCQCLAHAKAFETQWLENPTRFGFFQE"
     gene            1519..2034
                     /locus_tag="PKZ53_05225"
     CDS             1519..2034
                     /locus_tag="PKZ53_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39868.1"
                     /translation="MEFPPIIIAQSQGGRAIEKLYEKREFTDLMRQTVPECYRFAQLH
                     CPDIVLPEKYQFLIRHQTGTETFDRQAKTRTVPTHWICIIEQTVDVLLREDGWHLSDI
                     HLGVTAVSEDTTFIEVIWGIYGWTNRVLIEHPFFLFPPFIVMGPFLDPDGEEITARQS
                     RIYRLMVGPTS"
     gene            complement(2068..2532)
                     /locus_tag="PKZ53_05230"
     CDS             complement(2068..2532)
                     /locus_tag="PKZ53_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ39869.1"
                     /translation="MSDPLVETWEINCRINLYLLKAIPTDALALIPPSRGRSVGQAFA
                     HLHNVRLMWLKSAMPELLEGLQKIESDTAHDPELLCTSLTESSARMTELISTSLASGG
                     RVKGFKPHVQAFVGYLISHESHHRGQIMVALKDGGVKIDKKIPFGLWAWGVR"
     gene            complement(2664..3209)
                     /locus_tag="PKZ53_05235"
     CDS             complement(2664..3209)
                     /locus_tag="PKZ53_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39870.1"
                     /translation="MRKSFISILFMVGLCLMWTNPAIAIQEKKAQPRAKPVVYNVIPP
                     EAREFDFWVGKWRVTGPGTLPFTDTVKIAAGGTALIETWAAPDGSSSGSSINVYDSVN
                     KTWTQTYADSNGLFLQLTGKFENGEMILLGKFRNPANNRVQLGRLKFSNITKNGLDQD
                     MEVSDDNGQTWTLFYQTHWTK"
     gene            complement(3236..>4149)
                     /locus_tag="PKZ53_05240"
     CDS             complement(3236..>4149)
                     /locus_tag="PKZ53_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39871.1"
                     /translation="LCKGTLPTIRPKRAFQSVAVTIFLGLAVVAGMYQMSLEKHQKVG
                     GHNLRNWFTFQVRLPGGNTKDPVAVEQLLNETTQRIQSLDGVTAVCASSQFPFGPQKA
                     NWSIEIEGYRPEQTGKFPNHRVSAVSSGYFAAFQSRIQLGRDFTAEDRSSSERVMIIN
                     RAFADAYWPGENPLGRRLNLNPGDPWGWRTIVGVVENTSLSGTDDPVLPEAYCPIVQF
                     PPPILTFLVLSRGELPPLMLNPPRESQPAIPIFHLSSLNQRVTELLSRQHFALQSLLA
                     MIVAVMAIVVGHSGPTRSIQAASSQKK"
BASE COUNT         1110 a         1007 c          948 g         1084 t
ORIGIN      
        1 ttgagtcacc cgtacagaat tgcgtacaca acaaagattg tggtattttc gagatatgaa
       61 aacaattact gcgacaaaag cccgaaccaa ctttttcagc ttgcttgatg aagcagcgca
      121 aagccatgag ccgattcaaa ttaccggcaa gcggggaaat gcaattctca ttagcgagga
      181 tgattggcgg tctattcagg agacagttta cttgctttct atccccggga tgcgtgaatc
      241 catccgcgaa ggactggcga cgcctgtcga agaatgtgac cgggagcttg agtggtgagt
      301 aactggacac tggtctttac cagacaggcc caaaaagatg cgaagaaact ggcacaaact
      361 ggacttcggt caaaagctga agagctgctg gaaatcctca accgtgaccc gtttcaaaac
      421 ccgccaccct atgaaaaact ggttggcgat ttagccggag cgtattcccg tcgaattaat
      481 atccagcacc gcctggttta tcaggtttat caggatattc aaacggtcaa gattcttcgg
      541 atgtggaccc actacgaata aaaagtcagt cacttccctt tatctcaaaa ttcggggggg
      601 aaagaaaatc tttcaacttt taccagagat ttttgaccgt caaattgcca ggagagcagt
      661 ttccctttag gatcagggta ctccgcaaaa gccagacaat tgtcagttaa tgactgatat
      721 ctcgtttgca cgctgcctct tttgattggg ggaacctatg aaaattcttt ctgaatcatt
      781 gcctcaacgg tgcgaaatct gccaccagcg tgactgtttt aaccctcaga ccagttattg
      841 ctcaagatgt gaaaaagttc atacgttttt ggatcaaaac catcgaggaa tcctctccct
      901 tcctccgttt cttcaagatt tactccgaaa tcagcctccc aacccgttcg aaattctctt
      961 cggaaagaga tttcaaattc agccgagaca tctctccagg gcgttcatta tttcagtctg
     1021 tttgctggca gtggcttttt acttccatat cttttgtttt cttgccattt ttccacaaag
     1081 tcagcgattc atcacatttc aaggtggagc tttcgcaggt atgggcggtg gcacgctcta
     1141 tcattaccga accagcgatg gagaaaactt agggatgaga accttgttcg atggaccgat
     1201 ttccatcaag gaattgacag ccgtttctga tctacaactt gaagtgagcg tcgccagata
     1261 catatctgaa caaaatgtta ttgaatatga aagagtacat agccctcact ttccaaaggt
     1321 gatcgaaaag tacgttgcca ggaataccac ctcccagtta gctccatatt ccatttatta
     1381 catttacaac ggatactatc ttttcattat tgattgtcaa tgccttgccc atgccaaagc
     1441 atttgaaact cagtggttag aaaacccgac ccggtttggt tttttccagg aataatgagt
     1501 ggcaaaaggt gtcaacttat ggaattccca cctatcatca tagcccaatc ccaaggcggg
     1561 agagcgattg agaaactcta tgaaaaacgt gagtttaccg atctcatgcg ccagacggtc
     1621 cctgaatgct accgatttgc ccagttacat tgcccggata ttgtgttgcc ggaaaagtat
     1681 cagtttctca ttcgacatca aaccgggacg gagacctttg accgacaggc caaaacccga
     1741 acggttccaa cccactggat ttgcatcatc gagcaaacag ttgatgttct tttgcgtgaa
     1801 gatggctggc atctctcaga cattcatttg ggtgtaaccg ctgtttctga agacacaact
     1861 ttcattgagg ttatctgggg gatttacggc tggaccaacc gggttttgat tgaacacccg
     1921 ttctttctgt ttccaccatt cattgtcatg ggcccattcc ttgaccccga tggcgaggaa
     1981 ataactgctc gccagtcacg gatttaccgg ttgatggttg gaccaaccag ttgagtttat
     2041 gaggtatagc cacgacataa aacgtggtta ccgaacaccc cacgcccata aaccaaatgg
     2101 gattttctta tcaattttca ccccaccatc tttgagagca accatgattt gaccccggtg
     2161 atgagattcg tgagaaatca aatatccgac aaaagcctga acgtgaggtt taaacccttt
     2221 gacccggcct ccactggcaa gtgaggttga aatcaattca gtcattcggg cactggattc
     2281 ggttaatgac gtacacagga gttctggatc atgagcagtg tctgattcga ttttctgaag
     2341 accttcaagg agttcaggca tcgctgactt taaccacatc agccgaacat tgtgaagatg
     2401 ggcaaaagcc tgcccaacag atcgcccacg gcttggggga attagagcaa gggcatcggt
     2461 gggaatggcc ttcagtaaat acaagttgat gcgacaatta atttcccagg tttcaaccaa
     2521 tgggtcactc atcgttgtgg ctctcctctc gatggaaggt ataccatttt ggaatgatcc
     2581 tcttgtatga gcaaaaagct aagctattga taaggctgta cttccctcag cccttagccc
     2641 gataccctca gccctagatg gtattatttg gtccaatggg tttgataaaa caatgtccag
     2701 gtctggccgt tgtcgtctga aacctccatg tcctgatcaa gcccattttt tgtgatattg
     2761 gaaaacttca atcgcccaag ctgtacccga ttatttgccg gattgcgaaa tttcccaagt
     2821 aagatcattt ccccgttttc aaactttcca gtgagttgca aaaataatcc gttactatca
     2881 gcataggttt gggtccaggt tttattgacc gagtcataga cattgatgct tgagccactg
     2941 cttgagccat ctggcgcggc ccaggtttcg atcagagcgg tgcctccagc ggcgattttt
     3001 accgtatcag taaaaggaag cgttccaggt ccagtgaccc gccatttccc tacccaaaaa
     3061 tcaaactcac gagcttctgg tggaataaca ttataaacaa cgggtttggc cctgggttga
     3121 gctttctttt cttgaatggc gatggctgga ttggtccaca tcagacacag tccgaccatg
     3181 aacagaattg atataaatga ttttctcatt gtggaaaaac tcctagcacg cagtattact
     3241 tcttttgaga tgaagcggcc tggattgacc tggttgggcc ggaatgccca accacaatcg
     3301 ccatcacagc tacgatcatg gcaagcaacg actgtagcgc aaagtgttga cgactcaaga
     3361 gttcggtcac tctctgattg agagaactca aatgaaatat cggaatcgct ggttgagact
     3421 cacgcggcgg gttcagcatc agcggaggta attcgccacg gctcagaacc agaaatgtca
     3481 ggataggcgg tggaaactgg acaattgggc aataggcttc cggcaaaacc ggatcatctg
     3541 tgccagaaag cgaagtattt tcaaccactc cgacaattgt ccgccagccc caaggatcac
     3601 cagggtttaa attaagccga cgaccaagtg ggttctcacc cggccagtag gcatcggcaa
     3661 aggcacgatt gataatcatg acccgttctg aactggatcg gtcttcggcg gtgaaatccc
     3721 gtccaagctg aatccgggac tgaaaggcag caaaatagcc ggaactgacc gcactgactc
     3781 tatggttggg aaatttcccg gtctgctcag ggcgatatcc ttcaatttca attgaccagt
     3841 tcgccttttg gggaccaaac gggaactgac tggaagcaca aacagccgtc accccatcga
     3901 gagattgaat tcgctgagtg gtctcattca gcaattgctc caccgcaacc gggtcttttg
     3961 tgttccctcc gggtaatcga acttgaaagg taaaccagtt gcggagattg tgccctccaa
     4021 ccttctgatg tttctcaagg ctcatctgat acattccggc aaccaccgcc agacccagaa
     4081 aaatggtgac tgccaccgac tgaaacgccc gttttggccg gattgtggga agagttcctt
     4141 tgcacagtg
//
LOCUS       DAOQDO010000245         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_14887_length_4145_cov_3.212469, whole genome shotgun sequence.
ACCESSION   DAOQDO010000245 DAOQDO010000000
VERSION     DAOQDO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14887_length_4145_cov_3.212469"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKZ53_05245"
     CDS             <1..489
                     /locus_tag="PKZ53_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNJ39872.1"
                     /translation="PDQAGKLMMSLRIDEAAREMQAAIQGLLALPHTSGFSKVGSVGF
                     CMGGLLSLYAACKNPEIGACVIFYGGFPGVTPEIASLTSPVLGLYAGKDTFVTVASVH
                     QLEQILQHHQKPYSFHIYSDADHAFFNDTRPEVYREADARDAWERVLAFYQAHLWGGH
                     QG"
     gene            609..1079
                     /gene="ssb"
                     /locus_tag="PKZ53_05250"
     CDS             609..1079
                     /gene="ssb"
                     /locus_tag="PKZ53_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNJ39873.1"
                     /translation="MSFNKITIVGYLGRDPELRYTAQNVPVCTFSIATSERKRDAKSG
                     SDPAAESTTWFRVTLWREKAELASKYLAKGRQVYVEGRLSVREWQDRDGVTRTSLEVT
                     GTELHFIDTRNEAGGGAPSQGGRESLTRSSAAGSSAAHIDTDARAEVDEDDIPF"
     gene            1151..2857
                     /locus_tag="PKZ53_05255"
     CDS             1151..2857
                     /locus_tag="PKZ53_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ39874.1"
                     /translation="MNQTNLLSPRTIPLNLCLLIALAGLTFFFHLGSVGLLGPDEPRY
                     AQVAREMMDRGDWITPTLLGRTWFEKPALVYWLMGISYRLFGVSEWAVRFPSACLALG
                     GVLWMYWLGTRVHSQRFGFWTAAVLASSCFYFGFARGGTFDLPLTVCVSVTLGAFLWW
                     DLAPTRTNHGWLWVFYFFLGISLLAKGLIGLVLPAGIIGGYLSLSSTSVRDFFQRVNR
                     THWLLGSGLMLCSASIWYGPVIWQHGHAFIQEFFIAHHFQRYTSDKYHHTGPVYFYLP
                     VILGGLFPWTFFAVHGLLEQITTIRNQWTSPVEAKPSLPAFKVACFSLCWIFFPLLFF
                     SFSGSKLPGYILPIWPAAALMVGTSIEKLRADGCARTRQRLMAGTSLLIGLGAGLVWV
                     AGQNVVPVPGQFIGVVGVLGCLGSSGLILAFWPQSRAQSGFYGLLCGGMALLVIVITI
                     QLFPALERRESLKYLSVTARQAILPGELVTFYKCRKYAPVFYLPAQVHCCDIDQEPNR
                     YDHPDDLYTFLRSRGSLLVLLPASLEPELRTDPRFEVQPLGTEFTFSLIRLRPTRLSP
                     QD"
     gene            complement(2843..4048)
                     /locus_tag="PKZ53_05260"
     CDS             complement(2843..4048)
                     /locus_tag="PKZ53_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39875.1"
                     /translation="MNQSTGTRSLKPEPDAAPLRVLIVDDEADIRLICSEVLQEHYLV
                     KSVADGSEVPPMLTSFRPDIIVSDLKMPREGGLSVLRKAKQEVPLIEVLLITGYGRIE
                     DAIEAIQLGASDFLLKPFDINQLQTAVRRCADRIRLRRQNQDLQQANRELTKLNDLKE
                     KFLRLTSHELRGPLTVLQGYCDLLPLLADSPTEVVAATEAMSLAVNSLGSIVENLTTL
                     LSTYQEALLIIGQPLEIQKIVEQAVEEVRAYASHRGHTITIEDLDRMPPVIGDRLRLR
                     QILRELLLNSVKFTPDGGHIQLKIQRLAEPGWYALVVEDSGIGIEPSALSRIFEAFYE
                     QQNSIHHSTSRTNFRGGGIGVGLTLVREIVMAYGGRIGITSECGKGTEVVVSLPSLEP
                     GMPPASVLR"
BASE COUNT          846 a         1143 c         1080 g         1076 t
ORIGIN      
        1 cctgatcagg ctggaaaact gatgatgagc ctgcgcattg atgaggcggc ccgtgagatg
       61 caggctgcga tccagggact gctggcgttg ccacacacca gcggcttttc aaaagtcggg
      121 agcgtcggat tttgtatggg cggcctgctc tcactctatg ccgcctgcaa aaatcccgaa
      181 attggcgcct gtgttatttt ttatggtgga tttccgggcg ttacgccgga gattgccagc
      241 ctgacctcac cggtgctggg cctgtatgcc ggaaaagata cttttgtcac ggttgcttcc
      301 gttcatcagc ttgaacaaat acttcaacac catcaaaaac cctattcatt ccatatctac
      361 tctgacgctg accatgcctt ctttaatgac acgcggcccg aagtgtatcg agaagccgat
      421 gctcgtgatg cctgggagcg tgtactggca ttttaccaag cccatctgtg gggtggacat
      481 cagggctaag tcactgggct gaagcagccc ggcgaaactc tttgcccccc atcgcacagt
      541 ctcaaccctc aaaccagcct gggcaggccg ctcaggttct ttgattacgc gtaatccaag
      601 gagtaccaat gtctttcaat aagattacca tcgtcggcta tttaggacgc gaccctgaac
      661 tccgctatac cgcccagaac gttccggtat gcactttttc gattgcgacg tcggaacgca
      721 aacgcgacgc aaaatctggg agcgaccctg ccgctgaatc aaccacctgg tttcgagtca
      781 ccctctggcg cgaaaaagcg gaactggcca gtaagtatct ggcgaaggga cgccaggttt
      841 atgttgaagg gcgactttca gtccgtgaat ggcaggaccg ggacggcgtc actcgaacca
      901 gcctggaggt aaccggaacc gagctgcatt tcattgatac ccgaaatgaa gccggcggcg
      961 gggcacccag ccagggaggc cgggagtcat tgactcgctc atcagcggcg ggcagttcgg
     1021 cagcacacat cgataccgat gctcgggctg aagttgatga agatgatatc ccgttctagt
     1081 ttcaaccttg attttcaacc tgactggttg ccgggatggt atttcggcaa ccagtttttc
     1141 tttcattccc gtgaatcaaa ccaacctgct gtcgcctcgc accataccgc tcaatctctg
     1201 tttgctgatc gccctggccg gcctcacttt cttttttcac ctgggaagtg ttgggttgct
     1261 tgggccggat gagccacgat atgcccaggt cgcccgtgaa atgatggacc gcggcgattg
     1321 gatcactcca accttattgg gacgtacctg gtttgaaaaa ccagccctgg tttactggct
     1381 gatgggaatc agttatcgcc tgttcggcgt ttcggaatgg gcggttcgat tcccctcagc
     1441 ctgcctggcc ctgggcggtg ttctctggat gtactggctc ggcacccggg ttcacagcca
     1501 gcggtttgga ttctggaccg ccgcggtact ggcatcgtcg tgcttttact ttgggtttgc
     1561 ccggggtggc acctttgatt taccactcac ggtctgtgta tcggtgaccc tgggcgcctt
     1621 tctgtggtgg gaccttgctc caaccagaac caatcatggc tggctgtggg tattttattt
     1681 ttttcttgga atcagcctcc tggcgaaagg gctgattggc ctggtccttc cagcgggtat
     1741 tattggtgga tatctcagtc tgtcatcgac ttcagttcgt gattttttcc aacgagtcaa
     1801 tcgaacccac tggctgcttg ggagcgggtt gatgctgtgc tctgccagca tctggtatgg
     1861 gccagtgatc tggcagcatg ggcatgcttt tatccaggaa ttttttatcg cccatcactt
     1921 tcagcgctat acctccgata aatatcatca caccggtcca gtttattttt accttccagt
     1981 gattctgggt ggactgttcc cctggacgtt ttttgccgtc catgggttac tggaacagat
     2041 aaccacgatc cgaaaccaat ggacctcccc ggttgaagcc aaaccttccc ttcctgcctt
     2101 taaagtggcc tgtttctctc tctgctggat tttctttccc ctgctgttct ttagcttttc
     2161 tggttctaaa ttaccaggat acatccttcc gatctggccc gccgccgccc tcatggttgg
     2221 cacctcgatt gaaaaactca gggccgatgg ctgtgccagg actcgccagc gcctgatggc
     2281 tggcacctca ctcctgattg gtctgggtgc gggattggtc tgggtggccg gtcagaacgt
     2341 ggttcccgtt cctggccagt tcatcggggt ggttggggtg ctcggttgtc ttggaagcag
     2401 tggcctgatc ctggcttttt ggcctcaatc ccgggcgcaa tcggggtttt acgggcttct
     2461 ctgcggcggg atggcgctcc tggtgatcgt gatcaccatt cagctctttc cagccctgga
     2521 gcgtcgcgaa tccttaaaat acctttcggt gactgcccgg caggccatct tacctggcga
     2581 attggttaca ttttataaat gccgcaaata tgcaccggtg ttttacctgc cagctcaggt
     2641 ccattgctgc gatatcgacc aggagccaaa ccggtatgac cacccggatg acttatacac
     2701 atttctgcgt tccagaggga gcctgctggt ccttctccca gcctcgctcg aacctgaact
     2761 acggactgat ccgcggtttg aggttcagcc tctggggaca gaattcactt tctcactgat
     2821 tcgtcttcgc cccacacgcc tttcacctca ggactgaagc cggaggcatg cctggttcca
     2881 gagatggtag cgatacaacc acttcggtgc ctttgccaca ttcactggta attccgattc
     2941 gtcctccata cgccattaca atctctcgca ccagcgtcaa cccgacaccg atgccgccac
     3001 cacgaaaatt ggttcgggag gtgctgtggt ggattgaatt ttgctgttcg taaaacgctt
     3061 caaaaatccg actcagcgcc gacggctcga tgccaatccc tgaatcttcg accaccagcg
     3121 cataccagcc tggttcggcc agacgctgga tttttaactg gatatgccca ccgtccggcg
     3181 tgaacttgac ggaattcaac agcaattccc gcaggatttg ccggagccgt aatcgatcac
     3241 caatcactgg cggcatacga tcaagatcct ctatggtaat cgtatgtccc cggtgtgaag
     3301 cataggcgcg gacttcttca actgcctgct cgacaatctt ctggatctca agcggttgtc
     3361 caataattaa gagtgcttcc tggtaggttg aaagcagcgt cgtcaggttt tcaacaattg
     3421 aaccgagact gttcacggcc agcgacattg cttcagtggc ggccacgact tcggttggtg
     3481 agtcggctaa caatggaagc aggtcgcagt atccctggag aaccgtcagc ggaccgcgaa
     3541 gttcatggct ggtcagccgc aggaattttt ctttcaaatc attgagcttg gtcagttcac
     3601 gattggcttg ctgtaaatcc tgattttgcc gtcgcaatcg gatccggtcg gcacagcgtc
     3661 tgacggctgt ctggagctga ttgatatcaa agggtttgag tagaaaatcg cttgccccaa
     3721 gttgaattgc ttcaatcgcg tcttcaatgc gaccatatcc ggtgatgagc agcacttcaa
     3781 tcagtggaac ttcctgcttt gcttttcgca gaacgctcaa tccgccttca cggggcatct
     3841 ttaaatccga aacaataata tccgggcgaa aactggtcag cattggcggc acctcgctcc
     3901 catcagccac cgatttgacc aggtagtgtt cctgcagtac ttcagaacaa atcaaccgga
     3961 tgtcagcctc atcatcaaca atcagaaccc gtaatggtgc cgcatccggc tctggcttga
     4021 gagagcgagt tccggttgat tgattcatat ccatcataaa cattatgttc cagccaggaa
     4081 gggagtcgcg agcgtttgat gatacttcca gtcggatacc gcgtccagga tacgactggc
     4141 ggttt
//
LOCUS       DAOQDO010000246         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15009_length_4121_cov_2.884653, whole genome shotgun sequence.
ACCESSION   DAOQDO010000246 DAOQDO010000000
VERSION     DAOQDO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15009_length_4121_cov_2.884653"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKZ53_05265"
     CDS             complement(<1..1029)
                     /locus_tag="PKZ53_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNJ39876.1"
                     /translation="MTSIAEKIQQAQQFRHNPPLDLHFDTLREMVDSRVAHSPDAVYL
                     ISYDTAGNRTELTYGVFGERVRAGAALLAGLGVRRGDRVATYAANHAETVVAYFSALY
                     LGACLVPVNMTEDADRRKFIVENSQARVILALHQFTGLALSLAQEIPAIEHVVQLTGD
                     LVDGVVNWCEAQPVEAEFEAPSAQDESLIVYTSGTTGAPKGVVLLQSNLLADAHTLAM
                     WNHLEAKDRTMCVLPIHHVNGLVVTLLTPMYFGGSTVLNARFHTDYFWERIAAEQVRV
                     VSVVPTLLAFLLERPELFEADKVRSLAHIICGAGPLTVELGKAFEDAFGVRILHGYGL
                     SETTCYSSM"
     gene            complement(1165..2022)
                     /locus_tag="PKZ53_05270"
     CDS             complement(1165..2022)
                     /locus_tag="PKZ53_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ39877.1"
                     /translation="MKKSLVDPEIKSHLTTFQKLVSVSEAELDLAQAALQIAGIAYPD
                     LDPAPSLQKLAQLAETLRPRLANLSSTLEQAAEIARYLFVECGFTGNTDNYYDVRNSF
                     LNDVLERRLGIPISLSVVFIEVARRLGLHAEGVGMPGHFLVRHTSPDGEILLDPFHDG
                     KIIHPEDCQQLLTQVYGASAQMKPEYLRAVTHRQLLGRMLTNLKNIYINDENWPLMFQ
                     VIEMILLVQPEWWTEYRDRGVIQLRLENWSQAVDDLTLYCAKCPEDENFKILQEYIGQ
                     ARRALARWN"
     gene            complement(2034..>4121)
                     /locus_tag="PKZ53_05275"
     CDS             complement(2034..>4121)
                     /locus_tag="PKZ53_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39878.1"
                     /translation="VFVELANLQIVIDTLDTIIKSEEIRNLLKDKPEFAQLQQYETFL
                     AALGLPDKATLRDTRVAVAMRMPKPAAPPKGTKPGAKSPSPEPELVVFLEPPTVESAD
                     KIRVAFAGLAEKQGMAKPATVMVGKTKIMAYTEKQPVAYVQMGKVLAFGLLPMVKSVV
                     STVAPQTGAALNASTDFATIRQRVGAPQTLFAYVNTKIIGELINAGIEAFKTPPPVKK
                     GSKPVKKEPSAGDVALSNVGNYLGLPSLGGIGLAAGMTETEFRQRLYVAIDRKGKGLL
                     PVLADAPDLTLRGAALAPDSAQLFISFGFNHLRFYDEVLEAVRALPRKKDEPPPDAFI
                     AQGEAMAGFKIRDEFLAGFSGEHGVFINVGDVSQLIKNPGSSDLPKDARMAGFMVLSN
                     PGMVKAGVDKLLKLAAAEKKEGDAAEKKTGPTRETYKDIEIIQIEEVGISFIDDMLVI
                     GRANDVKWVIDSRAAGQTLEKNPSFAALRQKAPHNSMLSYYISEKFQQAMLEGFRKEA
                     APGVMELLSQMPSMAQFIHLQHDDQGVFMDATVPADVMKRAMAAIPSILAIQVLATAR
                     MNNSMSTRFVLHDLHTAMNLYQSGKGKGEYTDNLAVLGETGADGSSLLASDVVNMQKT
                     PRFGYLLGPIKLERDADGKLTRYSVVAFPAVKEGQNRTGDDCFYVDETGVVRHSKSAT
                     VMPDKNSEPAGSE"
BASE COUNT         1002 a         1133 c          993 g          993 t
ORIGIN      
        1 cattgaggaa tagcaggtgg tttcacttaa cccatacccg tgcaaaatcc ggaccccaaa
       61 ggcatcttca aacgccttgc ccagttcaac cgtgagcgga ccagccccac aaataatgtg
      121 tgccagcgag cgaactttgt cagcctcaaa cagttcgggt cgctccagta aaaaagccag
      181 caatgtcggc acgacgctca ccacccggac ctgttcagcc gcaatgcgct cccaaaaata
      241 atcagtatga aatctggcgt tgagaaccgt cgagcctccg aaatacatcg gggttaagag
      301 cgtcacgacc agcccgttga catggtgaat cggcaacacg cacatggtcc gatctttggc
      361 ttccaggtga ttccacattg ccagcgtatg ggcatcagcc agcagattgg attgcagtaa
      421 caccacgccc ttgggggcgc cagtcgtccc gctggtgtac acaatcaacg actcatcctg
      481 ggctgacggt gcctcaaatt cagcctccac tggttgagcc tcacaccagt tcacgacacc
      541 atcaaccaga tcaccggtca attgcaccac gtgctcaatt gcagggattt cctgggccag
      601 actcagggcc agcccggtaa attgatgcag cgccaaaatg acccgggcct gggaattttc
      661 aacaatgaat tttcggcggt cagcatcttc ggtcatattg accggtacca gacaggcacc
      721 gagatagagg gctgaaaagt aggccaccac cgtctcagca tggttggcag cataggtcgc
      781 cacccggtca ccacgtcgca cacctaatcc agctaacagt gcggcaccgg ctcgaacccg
      841 ttctccgaaa acaccatagg tcagttcagt tcggtttcca gccgtgtcat agctaatcaa
      901 atacacggca tcgggtgaat gtgccacgcg agagtcaacc atctcccgaa gcgtatcaaa
      961 atgcaggtca agcggtgggt tgtgacgaaa ttgctgagcc tgttgaatct tttcggcaat
     1021 tgaagtcata aggagtgaaa aagtaaagaa tgaagagtga ttgaaacgcg aagagtgaag
     1081 agccaatcca ggaaaagcga ggggcgacac cttgtctgac cactcgctct ttgcttctca
     1141 caactcacgc ttcaatcact tttgttaatt ccaccgggcc agggcccggc gcgcttgccc
     1201 aatatattcc tgaaggattt taaaattctc atcttctgga catttggcgc agtacagcgt
     1261 caggtcgtca accgcctgtg accagttttc aaggcgtaac tggatgacac cccggtcacg
     1321 atattcagtc caccattcag gctgcacgag caaaatcatc tcgatcacct gaaacatgag
     1381 cggccagttt tcatcattga tatagatgtt tttcagattg gtcagcattc gtccgagaag
     1441 ctgacgatgg gtaacggctc gcaaatactc tggcttcatc tgagcagatg cgccataaac
     1501 ttgcgtcagt aattgttgac agtcttcagg gtgaataatt tttccatcat gaaaagggtc
     1561 aagcagtatt tccccatctg gtgacgtgtg gcggaccaaa aaatgccctg gcatcccaac
     1621 cccttcggcg tggagaccca ggcggcgggc gacctcgata aacaccactg acagcgaaat
     1681 tggaatgccc agtcgccgct ccagcacatc gtttaaaaag ctattccgga catcgtaata
     1741 attatctgta ttcccggtaa atccacattc cacaaagaga tagcgggcga tttcagccgc
     1801 ttgctcaagt gttgaagaca ggtttgccaa ccgtggccga agcgtctcgg ccagttgcgc
     1861 cagtttttgt aacgaaggtg ctggatccag atcaggatag gcaatgcccg caatctgcaa
     1921 tgccgcttgg gccagatcca gttcagcctc cgaaacgctc accagttttt gaaaggtggt
     1981 gaggtgcgat ttaatttctg ggtcaaccaa cgactttttc atggtgtccc tggctattct
     2041 gacccggcag gctcactgtt cttgtccggc ataacagtgg cggatttcga gtgacgaaca
     2101 acacctgttt catcaacata gaaacaatcg tcaccggtcc ggttttgacc ttccttcacg
     2161 gctggaaagg caaccaccga gtagcgggtc agctttccat cagcgtcgcg ttccagtttg
     2221 attggaccca gcaagtaccc aaaccgggga gttttttgca tattcacaac gtcacttgcc
     2281 agaaggctgg aaccatcggc tccagtttcc ccaagcaccg ccagattgtc tgtatattca
     2341 ccctttccct tgcctgactg gtacaggttc atggccgtat gcaggtcatg caggacaaat
     2401 ctggttgaca tggaattgtt catacgagcc gttgccagaa cctgaatagc cagtattgag
     2461 ggaatcgccg ccatcgcccg cttcatgaca tcagccggaa ccgttgcgtc cataaacacc
     2521 ccctgatcat catgttgcag gtgaataaac tgggccattg atggcatctg gcttaacagt
     2581 tccataactc ctggtgcggc ttcttttcga aagccctcta acattgcctg ttgaaatttc
     2641 tcggaaatgt agtagctgag cattgaattg tgcggtgcct tttgcctgag cgccgcaaac
     2701 gaagggtttt tttccaacgt ttgaccggca gcccggctat caatcaccca tttcacatca
     2761 ttggcccggc caatcaccag catgtcgtca ataaaagaaa tcccgacttc ttcgatttga
     2821 atgatttcaa tgtctttgta agtctcgcgg gttggccccg tttttttctc agccgcgtcg
     2881 ccttctttct tctcagccgc ggccagtttc aagagtttgt caacgccggc tttcaccatg
     2941 ccggggttgc tcaataccat aaagccagcc atccgggcat ctttgggaag gtctgaggaa
     3001 ccggggtttt tgatcaactg gctcacatca ccgacattga taaaaacacc atgttcacca
     3061 ctgaaaccag ccagaaattc atctcggatt ttaaacccag ccatggcttc accctgggca
     3121 ataaaggcat ctggcggtgg ctcatctttt ttacgtggga gtgctcgaac ggcttccagg
     3181 acttcatcat aaaatcgcag atggttaaac ccaaacgaaa taaagagttg cgctgaatct
     3241 ggtgccaatg cggccccacg taacgtcaga tctggcgcat cagccagaac cggtaataac
     3301 cctttgccct tccgatcaat cgccacatac aaccgctgcc gaaactcagt ttccgtcatt
     3361 ccagccgcca gtccaatccc acctaaagag ggcagcccaa ggtaatttcc aacattgctc
     3421 agcgccacat ccccagccga tggttctttt ttgacgggct tgctgccctt tttgaccggt
     3481 gggggtgttt tgaaggcctc gatcccggcg ttgatgagtt cgccaatgat tttggtgttt
     3541 acataagcaa aaagggtctg gggggcacca actcgctggc gaattgtcgc aaaatcggta
     3601 ctggcattga gcgcggcacc ggtttgtggc gccactgttg aaaccactga tttcaccatt
     3661 ggcaacagcc caaaggccag aactttcccc atctggacat aggccactgg ctgtttctcg
     3721 gtataagcca tgatttttgt cttgccaacc atcactgtcg ctggctttgc cataccttgt
     3781 ttttcagcca atccagcaaa cgccacccgg attttatcgg cactttccac tgtcggaggc
     3841 tctaaaaaca ccaccagttc aggttccggc gaaggtgatt tcgcccctgg tttggtccct
     3901 ttgggcgggg cggcaggttt tggcatccgc atggcgaccg ccactcgggt atcacgcagc
     3961 gtggctttat cagggaggcc cagagcagcc agaaatgttt catactgctg aagttgggca
     4021 aattcaggct tatccttgag caggttgcgg atttcttccg atttgataat cgtgtccagg
     4081 gtatcgatta caatctgaag gttggctaat tcaacaaaaa c
//
LOCUS       DAOQDO010000247         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15113_length_4105_cov_2.550370, whole genome shotgun sequence.
ACCESSION   DAOQDO010000247 DAOQDO010000000
VERSION     DAOQDO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15113_length_4105_cov_2.550370"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1474)
                     /locus_tag="PKZ53_05280"
     CDS             complement(59..1474)
                     /locus_tag="PKZ53_05280"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNJ39879.1"
                     /translation="MAKIVSLCKRRGFVFPSSEVYGGLGSTWDYGPLGVELKNNVKRA
                     WWRANVYERDDMEGLDGAILMNRLVWQYSGHEATFSDPLVDCRECKGRFRADKLTELD
                     CPKHKQRKVSECSANLTEPRAFNLMFKTNVGPVEEDAALAYLRPETAQSMFVNFLNVV
                     NTRWRKVPFGIAQIGKAFRNEIVPGNFTFRTREFEQMEIEFFCKPGTDQDWHQRWIED
                     RHQWYLNLGIRPENIRRYVQQSDELAHYARACTDLQYRFFPEREDQEKQWDELEGIAN
                     RTDYDLSIHSKGKSEKWNKVNEHSTERLQYFDQEAKERYVPYVIEPSAGADRATLAFL
                     MDAYSEKAGKVQEGKDADMRVVLRLHPHLAPIKVAVLPLARNNSAIVETARKIKEELQ
                     KSGIRTVYDDTGAIGKLYARQDEIGTPFCVTVDFDTLGQGKDGSTADKDTVTIRDRDT
                     WEQIRLPINGLKAYFKERLDV"
     gene            complement(1791..3926)
                     /locus_tag="PKZ53_05285"
     CDS             complement(1791..3926)
                     /locus_tag="PKZ53_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39880.1"
                     /translation="MLSTAFKPLFQKGLACTVLCGVLVSATPVTALPVKQRLPIETSK
                     PAPTQEEPPVSTPQPPPAQTLTPMMVFEEHTELARIQGAATVDDRIQQLTEFQKQHPT
                     SPLITGCKTLLARAYAERGEQALRSAQVAQAQEAFQLAVDSLPTPISTATFRSFLLSM
                     PVLMAGFGNRTEAILFMRQLQERAGTEVELLEQIGLFYLSVENPTDALQVFDRAIELS
                     PTQANLHYDRGVALQMGLRLTDARTAFQKAMELDPKSRTAPAALADLYRASGLLGDAE
                     ALYRQQVVTVPDHPSAWGGLTLALMEQRRYGDAAETLGKMLAYTPRDVRFLTQMAYVY
                     AAQGEYASATKLLEKVREINPRYPWSRMVEAYLKRVEGDFADAENLLGEATDTARFPT
                     LYFELGKTLVQANDYERAIEPFEAFLRMSPEGEFEATLGGVLEVRGSNLAALLETERQ
                     AAILMNDPLTSEAQFRQIEDFVRFAYLAKREAQANAEKNQTADATPPPASTSPEGDQT
                     PSVRRPRRVVKPTAGDSTTAASWELPKPGDTPLGRALENFVSPKDDARGLRYLFAARK
                     LVDANAELPQAEALARGAALFASQATTREDAVADLPAGLSPEGRAQHFKLRAQAILGW
                     ILFREGKRDEAITALRTAAEGFPDGAEKKDASWKLGVVLEATGQLNDALVAYRSGYDS
                     NSTTAAIHRAIIEGVYQKINGSLDGLDLK"
BASE COUNT          885 a         1062 c         1136 g         1022 t
ORIGIN      
        1 ctgactactg actactgact actgactact gactactgac tactgactac tgactctcct
       61 aaacatccaa tcgttcttta aaataagcct tcaggccatt gattggcagt cgaatttgct
      121 cccaggtatc acggtcgcga atcgtgaccg tgtctttgtc agcggtactg ccgtccttgc
      181 cctggccgag ggtgtcaaaa tccaccgtga cacagaacgg cgttccgatt tcatcctggc
      241 gagcatagag tttgccaatc gcgccggtat cgtcatacac ggtgcgaatg ccgctctttt
      301 gcaactcttc cttaattttt cgagcggttt caacaatcgc tgaattgttt cgcgccagtg
      361 gaagcaccgc caccttgatc ggtgccaggt gtgggtggag tctgagtacc acccgcatat
      421 cggcatcttt gccttcctgg actttgccag ctttttcgct gtaggcatcc attaaaaatg
      481 ccagggtggc gcggtcggca ccggctgaag gctcgatgac atagggcaca tagcgttctt
      541 tggcttcctg gtcaaagtat tgcagccgtt cggttgaatg ttcattgacc ttattccatt
      601 tctcgctttt gcctttggag tggatgctca agtcatagtc ggtgcggttg gcaatgcctt
      661 ccagttcatc ccattgtttt tcctggtctt cgcgttcggg gaagaaccgg tactgcaaat
      721 cggtgcaggc ccgggcatag tgggccagct catctgactg ctgcacatag cggcggatgt
      781 tttccggacg aatgcccagg ttgagatacc actgatggcg atcttcaatc caccgctgat
      841 gccagtcctg gtcggtacca ggtttgcaga agaattcaat ttccatctgc tcaaattcac
      901 gggttcggaa cgtgaaattt ccaggcacaa tttcattgcg gaaggctttt ccaatctggg
      961 caatcccaaa cggcactttg cgccagcggg tgttgaccac attgagaaag ttcacaaaca
     1021 tactctgggc cgtttccggg cggaggtagg ccagggccgc gtcttcctca accgggccga
     1081 cgttggtttt aaacatcagg ttgaaggcac gcggttcagt cagattggcg ctacattctg
     1141 aaaccttgcg ttgtttgtgt tttgggcagt ccagttcagt gagtttgtca gcccgaaagc
     1201 gccctttgca ttcgcggcaa tcaaccagcg gatccgaaaa tgtggcttcg tggcccgagt
     1261 actgccacac caggcgattc atcagaatcg ccccgtccag accctccatg tcgtcgcgct
     1321 catagacgtt ggcccgccac caggcccgct tgacgttatt tttcaattcc acacccagcg
     1381 ggccgtagtc ccaggtcgag cccagtccgc cgtatacttc gctggatgga aacacaaatc
     1441 cccgtctctt acacaaagag acgattttcg ccatatcaac cgtcgtcata gattcctctg
     1501 ttcagaattg cttggtctgt gtgttgttga aggaagtggg tccaggtcag ggaagcacac
     1561 agcagcttaa gataagaaac cgcgcattat acacaaatac ctgtttgaat gaagaactgt
     1621 aaagacagaa atgaagaatg aagaatgaag aatgaagagt catcgggtac ttggtgcttg
     1681 gtgcttggtg cttggtgctt ggtgcttcga aaatattgat tcctaagcac taagcactaa
     1741 gcacattctc aattcttcat tcttcattct caattcttca ttcttcactc tcacttgagg
     1801 tcgagcccat cgagtgaacc gttgattttt tgataaaccc cttcaatgat cgcacgatgg
     1861 atggcagccg tggtgctgtt gctgtcatat ccagagcgat atgccaccag ggcatcattt
     1921 aactgcccgg tagcttcgag cacgaccccc agtttccacg aagcgtcttt tttctcagca
     1981 ccgtctggaa atccttcggc tgctgttcgc agggctgtaa tcgcttcatc gcgtttgcct
     2041 tcgcggaaaa gaatccaacc caaaattgct tgagcgcgga gtttaaaatg ctgggcccgg
     2101 ccttcgggag aaagtccggc tggcaggtca gccacggcat cttcgcgggt cgttgcctga
     2161 ctggcaaaca gcgccgcgcc acgggcaagg gcctcagctt gtgggagttc ggcattggca
     2221 tcaaccagtt tgcgggcggc aaacaggtat ctgaggcctc gggcatcgtc tttcggagac
     2281 acaaaattct caagtgcccg acccaatggc gtgtcgcctg gtttggggag ttcccaggag
     2341 gctgccgtgg tgctgtcacc ggcagttggc ttcaccaccc ggcgagggcg gcgaaccgat
     2401 ggtgtctggt cgccttcagg gctcgttgat gctggcggtg gggtggcatc cgccgtctga
     2461 ttcttttcag cattggcctg agcttcacgt ttggcgaggt aggcaaaccg aacaaaatct
     2521 tcaatctgcc ggaattgggc ttcactggtc agtgggtcat tcatcagaat cgctgcctga
     2581 cgttcggtct caagtaaggc agccagattt gagccgcgaa cttcaagcac gccacccaga
     2641 gtggcctcaa attcgccttc gggagacatt cgcaggaagg cttcaaacgg ctcaatcgcg
     2701 cgttcatagt cgttggcctg gacaagtgtt ttgccaagtt caaagtagag cgttggaaag
     2761 cgagccgtgt cagtggcttc accgagcagg ttttcagcgt cggcaaaatc accttcaacc
     2821 cgtttcaaat aagcctcaac catgcgtgac caggggtaac ggggattgat ttcccgaact
     2881 ttttccagaa gtttggttgc actggcatat tcaccctggg cagcatagac ataggccatc
     2941 tgggtcagaa agcgcacatc gcgtggggtg taggccagca ttttcccaag ggtttcagcc
     3001 gcgtcgccgt accgacgttg ttccatcaag gccagcgtca agcctcccca ggcggaagga
     3061 tgatctggaa cagtgacgac ttgctggcga tagagcgctt cggcatctcc cagcaatccg
     3121 ctcgcccgat ataaatcagc cagtgcggct ggtgcggtgc ggcttttcgg gtcaagttcc
     3181 atcgcttttt gaaaggcagt tcgggcatcg gtgaggcgca atcccatttg cagtgccacg
     3241 ccccgatcat aatggagatt ggcctgggtt gggctcagtt caattgcccg gtcaaatact
     3301 tgcagtgcgt cagttgggtt ttccacgctc aaataaaaca acccgatttg ttcgagtaat
     3361 tcgacttcgg tcccggcccg ttcctggagc tgacgcataa acagaatggc ttcggttcgg
     3421 ttgccaaaac cagccatcaa caccggcatc gagagcagaa aagaccgaaa ggtcgccgtg
     3481 gaaattggtg tgggaaggga atcaaccgcc agttgaaagg cttcctgagc ctgggcaacc
     3541 tgcgcactcc gcagggcctg ttcacctcgt tcggcatagg ctcgcgccag cagggttttg
     3601 cacccggtga tgagcggaga cgtcggatgt tgtttttgaa attcggtcag ttgctgaatc
     3661 cgatcatcaa ccgtcgctgc cccctggatc cgggccagtt cggtatgttc ttcaaaaacc
     3721 atcattgggg tcagggtttg ggccggaggt ggctgcggtg tggagacggg tggttcttcc
     3781 tgggttgggg ctggtttgga agtctcaatc ggcaatcgtt gtttaaccgg aagtgccgtg
     3841 acgggtgtgg ctgagacgag tacaccacac aacactgtgc aggcaagtcc cttctgaaaa
     3901 agtggtttga aagcagtgga taacatagat tgggtaccag cggtgaaaac acagaatatg
     3961 gattgcaggt gtgaaaagtt tggaatttgt accgtttgcg gggtgacttg gcaaggtaga
     4021 caggaagaat gaagcattga gaatgaagaa acgggttccg ggttccgggt tccgggttcc
     4081 gggttccggg ttccgggttc cgggt
//
LOCUS       DAOQDO010000248         4102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15127_length_4102_cov_2.511490, whole genome shotgun sequence.
ACCESSION   DAOQDO010000248 DAOQDO010000000
VERSION     DAOQDO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15127_length_4102_cov_2.511490"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(540..2120)
                     /locus_tag="PKZ53_05290"
     CDS             complement(540..2120)
                     /locus_tag="PKZ53_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNJ39881.1"
                     /translation="MSFQPIEFEVFKNLFVSIAEEMGVTLCRTAFSPNIKERLDFSCA
                     VYDAQGETIAQGDHMPVHLGAMPLSVQAAIEAVPMEEGDVVIVNDPFCGGTHLPDITF
                     VSPVFVPAKGTRGRRTLAFYVANRAHHSDVGGMSPGSMPLASELFQEGLIIPPIKLVS
                     RGKMVRDVLALILANVRTPTEREGDIAAQIAANRTAEQRLLHMVAKYGRGRVETQAAA
                     LQNYTERVMRATIATIPDGTYFFEDALDDDGFSQHPIRICATVKIAGDQAEVDFSGSD
                     PQTKGGVNANFAMTLSATLYAFRCLVTEDILYNAGIARAVKVLAPAGSIVHASRPAAV
                     AGGNVETSQRITDVLLGALAQALPDQIQAASQGTMNNVTLGGTDFRSGRPFAYYETLG
                     GGMGGRAGLDGLSGVHTHMSNTRNTPVEAIEHYLPVRIHQYGLRAGSGGQGQFRGGEG
                     LVREYEVLGETEVTVISERRTSAPYGLFGGEPGQPGCNLLIRDGIETHLPGKVNLKLN
                     AGDRLRIETPGGGGYGKN"
     gene            complement(2344..>4102)
                     /locus_tag="PKZ53_05295"
     CDS             complement(2344..>4102)
                     /locus_tag="PKZ53_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39882.1"
                     /translation="IWNETGDVLKFTLKPYFYQTTWAYLLFFGSILGLVVGGVRLRLN
                     SLKRHNEELEAKVRERTARLAETIDQLKLSEHEIQRKATELAETVEKLQLSERQALTA
                     KEDAIQSEKKALDANRAKSTFLANMSHELRTPLNAILGFVQIMQRRPLRDHEDQENLS
                     IILRSGEHLLGLINDVLSISKIEVGKITLSEQPFDLQKLLQTLQEMFRVRTQAKNLML
                     FFEIDPSVPQFVHGDEGKLRQILINLLGNAVKFTESGGITLRARWSGGMTHFEVQDTG
                     VGIAPEELSKLFEPFVQTRSGQKSAEGTGLGLAISSNFARLMGGEITVRSTYGKGSVF
                     SFAVELERVSGELVRNEERRVIGLAPGSPPYRVLVVDDKFENRALLLKLLIPIGFQLR
                     EAVNGKEAVEMAAEWNPHLIWMDIRMPVMDGYEATRKIREQEQESNQRLAATNGQFHR
                     KIIALSASVFEHDREAIFAAGCDDFLPKPFRENEAFDKMAHHLGITYVFDCEGETAPP
                     STPASGEIRVDDLARLPLVEIQKLSHALTIGDTEAATRLIEAIRHLNPELAVELTAMV
                     RNYQFDEILDLVEQVEKSE"
BASE COUNT          983 a         1086 c         1014 g         1019 t
ORIGIN      
        1 taaagaattg aaaatgaaga attgagaata aagagaatac ttgttgcctg gtacttagta
       61 caagtcccta taaatagggg atagaatttc ggttctttca tcgaatcaaa gcggttttca
      121 cccgagtggg cggtaagcac atcaaatccg ctttcatccc ttactcatag ggacttgtac
      181 ttaggaatca attctttcga agtagtacca ggtaccaaat agttcttcat tcttcattct
      241 tcattccctc aattttgcct aaaatatact catttcctca gttttggtca caactgtaat
      301 tgatcgagcc cgaaccaaag gacatcaagg acaaaaaaga caaaaaggac cgggtgggag
      361 tcagttttgt caccgcaaag gctcgatttc agtgaaattc gagttcactc aatgagcttg
      421 cagactggtg cctgccagtg gtgactcgga cgggtcggcg ttgatcgttg agcaaggtgg
      481 agctattttg ggcaaaattc aggttcattc ttcattcttc attcttcatt cttcattctt
      541 caattcttcc catacccgcc accgcccggc gtttctatcc gcagacggtc accggcgttg
      601 agtttgagat ttaccttgcc gggaaggtgt gtttcaattc catcgcgaat cagaaggttg
      661 catccgggct ggcctggttc tccgccaaat aatccatatg gggcactggt tcggcgctcg
      721 gaaatgacag ttacttcagt ctcacccagc acttcatatt cacgaaccag cccttcgcca
      781 cctcgaaact gtccctgtcc accgcttccg gctcgcagcc catactggtg gatccgaact
      841 ggtaaataat gttcaatggc ctcgaccggg gtattacggg tgttgctcat atgggtgtgg
      901 acgccactga gcccatccaa cccggctcga ccgcccattc caccacccag cgtttcatag
      961 taggcaaagg gtcggccaga tctgaaatca gtgcctccga gcgtcacatt gttcatcgtt
     1021 ccctggctgg ccgcctggat ttgatccgga agcgcctgag ctaaagcacc caggagcaca
     1081 tcggtaattc gttgcgaggt ctcaacgttg ccaccagcca cggcggcagg tcggctggca
     1141 tggacaattg atccggctgg ggccaggacc ttcaccgccc gggcaatacc ggcgttgtac
     1201 aaaatatctt cagtgactaa acatcggaag gcatacagcg ttgccgataa cgtcatggca
     1261 aaattggcgt tcacaccacc tttggtttgc gggtcactcc ctgagaaatc aacttcggcc
     1321 tggtcacctg caattttgac agtggcgcaa atccgaattg gatgctgact aaatccgtcg
     1381 tcatccagtg catcttcaaa aaaataggtt ccatctggaa ttgtggcaat cgtcgcccgc
     1441 atgacgcgtt cggtgtagtt ctggagcgct gccgcctggg tttcaacccg cccgcgtcca
     1501 tatttggcaa ccatgtggag caatcgctgc tcagccgtgc gattggcagc aatctgggcc
     1561 gcaatatcac cttcgcgttc ggttggtgtg cgaacattgg ccagaatcag cgccagaaca
     1621 tcacgaacca tcttcccccg gctgaccagt ttaatgggtg gaatgatcaa cccttcctga
     1681 aataattctg acgccagcgg catagatcca ggactcattc cgccgacatc cgaatggtgt
     1741 gcccggttgg cgacataaaa agccagggtc cggcggccac gcgttccctt cgctggtaca
     1801 aaaactggcg agacaaacgt gatgtctggt agatgggtac caccgcagaa ggggtcattg
     1861 acgatgacca catcgccttc ttccattgga accgcctcaa tcgcagcctg gaccgaaagc
     1921 ggcatagcgc caagatgcac tggcatatga tcgccctgag caatcgtttc gccctgggca
     1981 tcatagaccg cacatgaaaa atcgaggcgt tctttgatat tgggggaaaa cgccgtgcga
     2041 cacagtgtca cacccatttc ttcggcaatg gaaacaaaaa gatttttgaa gacttcaaat
     2101 tcgatgggtt ggaaagacat ggatttaggg gttcggggtt tggggtttgg ggttttggag
     2161 aaggacaaaa aagacaaaaa ggacgaaaag gacgaaaaaa caggagagca ggagatatga
     2221 cggctttgaa tgacgcctgc ttcgaaagcc cggaacccgg acaagatgac tgacaaggtg
     2281 acaaggtgat ttctttcatc cctcatcctt catccctcat cccttccgaa aacccggaac
     2341 ccgctactca ctcttctcca cctgttcgac cagatccagg atttcgtcaa actgataatt
     2401 tctgaccatg gcggtcaatt caactgccag ttccgggttg agatggcgga tggcttcaat
     2461 caaccgggtt gcggcttcag tgtcaccaat ggtcagggca tgcgagagct tctggatttc
     2521 aaccagcggc aaccgggcca gatcatctac cctgatttca cccgaagctg gggttgatgg
     2581 cggtgctgtc tctccttcgc agtcaaacac ataggtgatt ccaaggtggt gcgccatttt
     2641 gtcaaaggct tcattttcac gaaatggttt ggggagaaaa tcgtcacatc cagcggcaaa
     2701 aatcgcttcc cggtcatgct caaacacact ggctgagagg gcaatgattt ttcgatggaa
     2761 ttgcccattc gttgcggcga gtctttggtt tgactcctgt tcctgttcac ggattttccg
     2821 tgttgcctca taaccatcca ttaccggcat gcgaatatcc atccagatca gatgcgggtt
     2881 ccactcggcg gccatctcaa ccgcctcttt tccattgacg gcttcgcgaa gctgaaaacc
     2941 aatgggtatc aataatttga gcagcagcgc ccggttttcg aatttgtcat ctaccaccag
     3001 gactcgatag ggagggctcc ctggcgccag cccaatcacc cgccgttctt catttctcac
     3061 cagttcgccc gacacacgtt ccagttcaac ggcaaaagaa aataccgagc ctttgccata
     3121 ggtactgcgg acggtgattt ctccgcccat caacctggca aaattagaac taatggccag
     3181 ccccaggccc gtcccttcag ccgatttctg gccgctccga gtctggacaa atggttcaaa
     3241 gagctttgaa agctcttcgg gggcaattcc gacgccagta tcctgaacct caaaatgggt
     3301 catgcctccg ctccatcgag cccggagcgt aatcccgccc gattcggtaa atttgacggc
     3361 attgcccagc aggttgatca ggatttgccg gagcttccct tcatccccat gcacaaattg
     3421 gggcacagac ggatcaattt caaagaacag catcaggttt tttgcctggg tgcgaacccg
     3481 aaacatctcc tgcagggttt ggagcaattt ttgaagatca aacggctgtt cggaaagggt
     3541 gatttttcca acttcgattt ttgagatgga aagcacatcg ttgatcagtc ccagcaggtg
     3601 ttcaccactg cgcaggataa tactcaggtt ttcctggtct tcatgatcac ggagcggacg
     3661 gcgctgcata atctgcacaa atccaagaat ggcattgagc ggtgtgcgca attcgtggct
     3721 catattggcc agaaacgtgc tttttgcccg gttggcgtcg agggcttttt tctcagattg
     3781 aatggcatct tctttggccg tcagggcctg acgctcggaa agttggagtt tctcaacggt
     3841 ttccgccagt tcagtcgctt tgcgttgaat ttcatgctct gagagtttga gttgatcaat
     3901 cgtttctgcc agacgggcgg tacgttccct gactttagct tccagttctt cattatgacg
     3961 cttgagggag ttgagcctga gccggacacc tccgaccacc agccccagaa ttgatccaaa
     4021 aaacaagaga tacgcccagg tggtctgata gaaatacggc ttgagcgtaa atttcaggac
     4081 atctccagtt tcattccaga tc
//
LOCUS       DAOQDO010000249         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15130_length_4101_cov_2.080573, whole genome shotgun sequence.
ACCESSION   DAOQDO010000249 DAOQDO010000000
VERSION     DAOQDO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15130_length_4101_cov_2.080573"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..776)
                     /locus_tag="PKZ53_05300"
     CDS             complement(228..776)
                     /locus_tag="PKZ53_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39883.1"
                     /translation="MTLNKLLFTLLLAGLVSGFTSDLQAAFPIQSKPTATQVSTRAAG
                     GKQTLTGSWMATFSSADNPPSFPPLPALFTFAKDGTLIQIDGGELQPSPEDFPYYSSP
                     GHGAWKKMSGSQYAFAFMIIAVNPDGTLNSTGKVKFTIDLSADGQSFSGNGTFAFFDA
                     DGVPIEGASGEEKITGQRLNVE"
     gene            complement(819..1088)
                     /locus_tag="PKZ53_05305"
     CDS             complement(819..1088)
                     /locus_tag="PKZ53_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39884.1"
                     /translation="MRKKITITIETQRTVSVRREPNRRWEWCAQCQEYVDLVESEQVA
                     LILGVCPQTLGPLTETGVIHPPGQTSEAGLICLNSVLQYHGTDPK"
     gene            1284..3497
                     /locus_tag="PKZ53_05310"
     CDS             1284..3497
                     /locus_tag="PKZ53_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ39885.1"
                     /translation="MSKQAKHFYEFGTFRVDPQDRLLLKEGKVVPLAPKVFDILLIFL
                     EHPGSVLEKENLLERVWPDTFVEEGNLARNVSTLRKVLGEVDEGAPFIETIPRRGYRF
                     VASVKSLADDNLTVIVQERSHITIEEEVEFDQSTTVSNQNGNSSALTDLGQLPEKLAQ
                     PLPAVSESGSLQRQPQVSFSLSKMAIWSLFIGVIGVCVGAGFLQRPQSTGSLPAFRQV
                     SFRNGMVWSARFLKDGATILYNAAWEGNPGEILSLRPENPESRPFGLSDVYLASVSVT
                     GELAIITHPKSMAHRGTLARVSFSGSGAPREILNDVFEADWSPDGKELAVIHFEDGKF
                     QLQFPIGKTLYEAPLPGWISNVRISPTGEWIAFINHPTQAYDMSGAVFLIDRNGKSRQ
                     LTSDWTSVSGLAWSPSGTEVWFTSSAIGTNCDLNAVNLNGQERQLVRVPGSLTIYDIS
                     AQGQVLLAREDWRTGIMCFPPGATQERDLSWLDGSWLRDLSTDGQQILFDEEGEGGGK
                     TASVYLRHTDGSPAVRLGDGFAISLSPDGKWALARYRHTKPPQLVLIPTGVGQPQVIP
                     TGDIEFREYGNWFPDSKRLVLFGREAGHKPRLYTFDLEAKIVQPLTPEGITGRPLSPD
                     GRTIVVNDASRNRFWFSVETGEMRPFPVLEPHTGIICWSADGRSLFLVQGENPLRISR
                     FDIETGHKEFLREIQYFDQAGLISMIPILYSADGKSYAYTYVRKLSNLYLLDGVR"
     gene            complement(3513..3929)
                     /gene="arr"
                     /locus_tag="PKZ53_05315"
     CDS             complement(3513..3929)
                     /gene="arr"
                     /locus_tag="PKZ53_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HNJ39886.1"
                     /translation="MATSDNPDSQTYYHGTKADLKVGDLIEPGYASNYGKRKQAAFVY
                     LTATLDAAIWGAELAVGAGPGRIYIVEPTGSIEDDPNLTNKRFPGNPTKSYRSRNPFR
                     VIGEVTDWQGHSPDQLKEMHDHLENLKQRGIEAIEE"
BASE COUNT          974 a          960 c         1074 g         1093 t
ORIGIN      
        1 tattgcattg actgcctgtg tcgtgccagc cgttcgagcc atgcgcatta gcccaacgat
       61 tgcactccgc tatgaataaa ggtgcatcgc tatcagagcg aagtagcgaa tagcgaaata
      121 gtgaaaatac aggagttaag attgacagat ccgttttgag aacgaatgct tcgctacttc
      181 gctacttcgc tacttcgcta cttcgctact tcgctacttt gctcagttca ttccacattc
      241 agccgctgac cggtgatttt ctcctcgcct gacgctccct caatcggaac cccatccgca
      301 tcaaaaaacg caaaggtccc attcccgcta aatgattggc catcagcact taaatcaatt
      361 gtgaatttga ctttcccggt actattgagt gttccatccg gattgacggc gataatcata
      421 aaggcaaagg catattgaga gccgctcatt tttttccagg cgccatgacc tgggctggaa
      481 taatagggaa aatcttctgg tgatggttgt aactccccac catcaatctg aatcagcgtt
      541 ccatctttgg caaaagtaaa caatgcaggc agtggtggaa atgacggcgg gttgtcagcc
      601 gatgaaaacg tcgccatcca gcttcccgtc aatgtttgtt tgccacctgc cgctctggtt
      661 gagacctgag tggctgttgg tttggattga attggaaatg ctgcctgcag gtctgaagta
      721 aatccggaaa ccaatccagc caaaagaaga gtaaaaagaa gtttgttcaa cgtcatacca
      781 atggtgctcc ttaaattggg attctgaggt gataggtgtt atttggggtc ggttccgtgg
      841 tactgcagca ctgaattaag acagatcaac cctgcctcac tggtttggcc aggcggatga
      901 atcactccgg tttcagtaag cgggccaaga gtctggggac agactccaag gatgagcgcc
      961 acttgttcac tctcaaccaa atcaacgtat tcctggcatt gagcacacca ttcccatctg
     1021 cgattgggtt cacgtctgac tgacactgtc cgctgggttt cgatggtaat ggtgattttc
     1081 tttctcatag cacctgattc acgccttttg attttagaaa gtcaggctga ctgcttcagt
     1141 tttggtagaa aaccgacggc agcgaccata aggaaaatct tgaaaatttg tctttcgcaa
     1201 actcggaaaa aggtatgaaa tgagttcgga acgagtttgg aatgagttcg gaagaaacct
     1261 ggaatgaatg agttgaggaa cttatgtcaa agcaagcaaa gcacttctat gaattcggaa
     1321 catttcgggt tgatcctcag gaccggttgt tgctcaaaga ggggaaggtt gttcctctgg
     1381 cgccaaaggt ctttgacatt ttattgatct ttttggagca ccccggcagc gtcctggaaa
     1441 aagagaattt gctggagcgt gtctggccgg acacgtttgt tgaggaggga aacctggcgc
     1501 gaaacgtctc gacacttcgc aaggtgctcg gagaagtgga tgaaggggca ccgtttattg
     1561 agacaatccc acgtcgtggc tatcgctttg tggccagcgt caaatccctg gccgatgata
     1621 atctaactgt gattgtgcag gagcgatccc acattaccat cgaagaagaa gttgaatttg
     1681 atcagtccac gaccgtttcc aaccaaaatg gcaattcctc tgccttaacc gatctgggcc
     1741 aactgcctga gaaactggcg caacctcttc cagccgtttc agaatcaggt tccctgcagc
     1801 gacaaccaca ggtttcattc agcctgtcaa aaatggccat ctggagctta tttattggtg
     1861 tgattggagt ttgtgttgga gctggttttt tgcaacgtcc ccagagtaca ggctcattac
     1921 ccgcatttcg acaggtgagt tttcggaatg gaatggtgtg gtcggctcgc tttctcaaag
     1981 acggagcgac cattctctac aacgccgcct gggaagggaa tccgggtgaa attttgtcgc
     2041 ttcgtccaga aaaccctgaa tcacgtccat ttgggttgag cgatgtctat ctggcttcag
     2101 tttcagtgac cggagaactg gcgattatta cccatcccaa atcaatggcg catcggggca
     2161 cgctggcacg cgtttcattt tctggaagtg gggcgccgcg tgaaattctc aatgacgtgt
     2221 ttgaggccga ctggtcaccc gatggaaaag aactggcggt gattcacttt gaagacggaa
     2281 aatttcagct tcaatttcca attggcaaaa ccctgtatga agcaccattg ccgggttgga
     2341 tcagtaatgt tcggatttca ccaactggtg aatggattgc atttatcaat catccgaccc
     2401 aggcttatga tatgagtggg gcagtttttc taattgaccg gaacggtaag agtcgccagc
     2461 ttaccagcga ctggacttcg gtcagcggtc tggcctggtc gccctccggc acggaagtct
     2521 ggttcacctc ttcagccatc gggaccaatt gtgatttgaa cgctgtcaat ctgaatggac
     2581 aggagcgcca actggttcgg gtgccgggca gtctgacgat ctatgatatt tctgcccagg
     2641 gacaggtttt gctggcccgc gaagactggc gcactggcat tatgtgcttt ccaccaggag
     2701 cgactcagga gcgggatctg tcgtggcttg atggttcgtg gctacgggat ctttcgacgg
     2761 atgggcaaca aatcctgttt gacgaagagg gagaaggtgg tgggaaaacg gcgtcagtat
     2821 acctgcgcca cacagatggc agtccagcgg ttcgactcgg agacggtttt gcaatttcgc
     2881 tgtctccgga cgggaaatgg gcactggcgc gataccggca caccaaaccg ccacagttgg
     2941 ttttgattcc aaccggtgtt ggtcagccgc aagtcatccc gactggggac atcgagtttc
     3001 gcgaatatgg caattggttc cccgacagta agcggctggt gttatttgga cgtgaagcgg
     3061 gacacaagcc acgactctac acttttgatt tagaggctaa aattgttcaa cctctgactc
     3121 cggaagggat taccgggcgt ccgttgtcgc cggacggacg gaccatcgtc gtaaatgacg
     3181 ccagccggaa tcggttctgg ttttcggttg agaccggaga aatgcgtccc tttccagtgc
     3241 ttgaaccgca taccggaatt atttgttgga gcgccgatgg ccgttcgctc tttctggtgc
     3301 agggagaaaa tccattgcga atctcacggt ttgacattga aaccggccac aaagagtttc
     3361 tgcgggaaat tcagtatttt gaccaggcag gattgatttc aatgattccg atattgtatt
     3421 cggcagacgg taaatcctat gcctacacgt atgtgcgcaa actctcgaac ctgtacctgc
     3481 tcgatggtgt gcgatagatg gtcgaaccgg gcttattctt caattgcttc aatcccacgt
     3541 tgcttgaggt tctcaaggtg atcgtgcatt tctttgagct ggtcagggga gtgaccctgc
     3601 caatccgtaa cctcgccgat gacgcgaaat ggatttcgag aacggtatga cttcgtagga
     3661 tttccgggaa atcgtttatt cgtaaggttc gggtcatctt caatggatcc ggttggttcc
     3721 acgatataaa ttctgccagg ccctgcgcca acggccagct cagctcccca gatagccgca
     3781 tccagtgtgg ccgtcaaata aacgaaagcc gcctgtttcc gtttgccata gttggaagca
     3841 tagcccggtt cgatcagatc acccaccttc aggtcggctt ttgtgccgtg gtagtacgtt
     3901 tgtgaatcag gattgtcgct ggtagccatt tattactcca gatgttgagg ggattggcca
     3961 gatgttattt atcattacaa cggccaggaa atagtgtggt gctatagctt ttcagataaa
     4021 tatttcttgg gtttctatgt cttgagattt cataaactat tgaagaattg ggatttattt
     4081 tctgtgtatt ccgtgtattc c
//
LOCUS       DAOQDO010000250         4092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15190_length_4092_cov_3.777310, whole genome shotgun sequence.
ACCESSION   DAOQDO010000250 DAOQDO010000000
VERSION     DAOQDO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15190_length_4092_cov_3.777310"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..3931)
                     /locus_tag="PKZ53_05320"
     CDS             complement(134..3931)
                     /locus_tag="PKZ53_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39887.1"
                     /translation="MRWNGLLLICAFVIGLLSPDKTVAQIYRFTSYTASMGLPGNTIY
                     CLYQDRQGYMWFGTDSGACRYDGANFTSFGVQQGLADSSVRTIFEDRKGRLWFCTMRG
                     LSCFEQGKFTNYTTAQGLVDDHVYSGLEAPDGSLWLGTAAGLSHFDGQRFVHFGKEAG
                     LPNWPVWSLAQDSAGIIWFVTRGGGAGKFDGTRATVFTLADGLPGESAFDVDIDPAGG
                     VWFATNGGPCFFDGAKFRTYTTKDGLNSPQAGKILIDRYHRIWCAMFGGGINRLDPDG
                     VKIFDRTHGIPNDLLTAVLEDYEGNLWFGTQSNGAFRFSSELFASYTAAHGLGDGRIS
                     GIAETPDEQVWIATATNGLFALNPKTGAARHFGTKDGLLEETLWSLFADSRHRIWVGG
                     YKGVTCLDGQNIETFTREQMGLRERVTAITEDQQGRLWFGSYPTTSNGIAIYDNQKFS
                     LMTTQQGLLQNPINTLTRGPDGSIWVSCDKGLSHFDGQQFSHFRIGQGLPGNRVQCAY
                     FDSTGTLWVGTDGGISRLTGTTFTSFTTTDGLPSNSIRAIVGGDKTLWIGTTRGISAY
                     DGQTFRNYSTKDGLASDDVGFGLAIRRRDGTIWFGTGEGVVRYRAALDIARAITPRLG
                     LAPVLVEEKPVQLVSGAAGNAFQLPVLSYQQNDISFEFLCLSFTDETAIRYSYQLEGL
                     DHNWSVETANRIARYANLTPGDYRFRVKARAASGVWSEVSTIEFAIKPPFWKTWWFQL
                     AGIFLVSSSIYGGYMLRIRGLRLREEQRIAHLQQLQEQKLEHLRQLQQQRVEYLSQIH
                     EQRLEGMRQLLESIRLINSKLDLTIVLQNIVEESARLVGGEPGGIGLLEGDEIRFHRL
                     WAADHWEDSLLTFKIGEGVAGRVAESKQTMIVDDPHHSPYITQPETLDVYHVEGMMEV
                     PIVSRDGQVVGVLDIRHRAGSPPFNDNDRQLVESLANQAAVALENAALYGVVEEKKNE
                     LEAKNLVIVESMNELEKLYKQEHEFAGKLKELDQMKTNFMIVTSHEMRTPLTVLRGYC
                     EALLDEYLGPLTEAQRVSLTTCHRMVDRLTRSFNDILEMVKINAGQITLKPKVFHLNA
                     AVNNVLTELHTFIEQRKQQVKLQIPEQIIISADCEKIELVLINIIQNAIKFTPDYGEI
                     LIAVTTETECIHILVQDSGIGIDAVDLTHIFDKFYTNQDPSTHRSGRFEFSARGTGLG
                     LAIAKSYVEAHNGQIWAESNGVGTGSTFHIVLPIGVRPDTMTSELTDLRNRAEEVGLK
                     KTG"
BASE COUNT         1007 a         1139 c          955 g          991 t
ORIGIN      
        1 gctcggggca atcgaaggga tgagggatga aggatgaggg atgaaataaa accaacttct
       61 tcagcccgac ttcttcagcc cgttttcttc agcccgtttt cttcagccct ggttttcttc
      121 agccctggtt ttctcagcct gtcttcttca gcccaacttc ttcagcccga tttctcaaat
      181 cagtcaattc actggtcatg gtgtctggac gcaccccaat cggtagcacg atgtgaaacg
      241 tgctgccggt tccgaccccg tttgactcag cccagatctg accgttatgc gcttcgacat
      301 aactttttgc aatggccagc ccaagacctg ttcctcgggc tgaaaattca aaccggccag
      361 accgatgtgt cgaaggatcc tgattggtgt agaacttatc aaaaatatgc gtcaggtcca
      421 ccgcgtcaat cccaatacca gaatcctgga ccaggatatg gatgcactcc gtctcggtgg
      481 taacggcgat caggatttca ccatagtctg gggtgaactt aattgcattt tgaatgatat
      541 tgatcaagac cagctcaatt ttttcgcaat ccgcagagat aattatctgt tctggaatct
      601 gtaacttcac ctgttgcttg cgttgctcga tgaaggtgtg caactcagtt aagacattgt
      661 tgacagcagc gttcaaatga aaaacttttg gtttcagggt gatctgaccg gcattgattt
      721 tgaccatttc cagaatatcg ttaaagctcc gggtcaaccg atcaaccatc cggtgacagg
      781 tggtcaatga cacgcgttgg gcttcggtta agggcccaag atattcatcg agcagtgcct
      841 cgcaataccc acgcaggacc gtgagtggtg tccgcatttc gtgcgaggtc acgatcataa
      901 aattggtctt catctggtcc agttctttga gtttgccggc aaattcgtgt tcctgtttat
      961 agagcttttc gagttcgttc attgactcga caatgaccag gtttttggcc tccagttcat
     1021 ttttcttttc ttcgaccaca ccatagagcg cggcattttc aagtgccacg gcggcctgat
     1081 tggccaggga ttcgaccagt tgtcggtcat tgtcgttaaa gggtggggat cctgcccggt
     1141 gacggatatc aagcacgccc accacctgac catcccgcga cacaatcggg acttccatca
     1201 ttccttcgac atgatagaca tcaagtgtct caggctgagt gatatatgga gagtggtgcg
     1261 gatcatccac aatcatggtt tgttttgact ccgcgactcg accagccacc ccttcgccaa
     1321 ttttgaaggt gagcaggcta tcctcccagt gatcggcggc ccacagacga tgaaaccgaa
     1381 tttcatcccc ctccagtaaa ccaatcccac ccggttcacc gcccaccagt cgggcacttt
     1441 cttcgacgat gttttgcaaa acaatcgtca gatcaagctt ggaattgatc aaccgaatac
     1501 tttccagcaa ttgccgcatt ccctccaacc gttgctcatg aatctgagac aggtattcaa
     1561 cccgttgctg ctgaagctga cgcaaatgtt cgagtttttg ctcctgcaat tgctggaggt
     1621 gggcaatgcg ttgctcttcg cggagccgca acccacgaat ccgcagcata tagccaccat
     1681 aaatgctgct actcaccagg aaaatcccag ccagttgaaa ccaccaggtt ttccaaaatg
     1741 gaggcttgat cgcaaattca attgtggaaa cttcgctcca caccccggaa gcggctctgg
     1801 ctttgacccg aaaccgatag tcacccggtg tcaaattggc atagcgggca atccggttgg
     1861 ccgtttccac tgaccagttg tggtctaagc cttcaagttg atagctatag cgaatcgccg
     1921 tttcatccgt gaacgacagg cacagaaatt cgaaagaaat gtcattttgc tgatatgaga
     1981 gtaccggcaa ctgaaacgca ttgccggctg ccccagagac aagctgaacc ggcttttctt
     2041 caaccagcac gggtgccagt cccagtcgtg gggtgatcgc tcgggcaatg tcgagcgcgg
     2101 cccgatagcg caccacgcct tcgccagttc caaaccagat cgttccatca cgccgccgga
     2161 tagccagtcc aaacccaaca tcatcactgg ctaatccatc tttggtcgaa taattgcgaa
     2221 acgtttggcc atcataagct gagattccac gggtggtgcc aatccacaac gttttatcac
     2281 cgccaacaat ggcacggata gagttgcttg gcagtccatc agtcgtggtg aaactggtaa
     2341 atgtggtgcc tgtcaatcgt gatatcccac catcggtccc gacccagagc gtgccggttg
     2401 aatcaaagta ggcacattga acccgatttc ccggcaaccc ctgtccaatt ctgaaatgcg
     2461 aaaattgctg cccgtcaaaa tggctcaatc ccttatcaca ggaaacccaa attgatccgt
     2521 ccggcccacg ggtcagggta ttgattggat tttggagtaa tccctgttgc gtcgtcatca
     2581 aggaaaattt ctgattgtca tagatcgcaa ttccattgct ggtggtcgga tacgagccaa
     2641 accagagtcg cccctgttga tcttcggtga tggctgtcac gcgctcacgt aatcccattt
     2701 gttcacgcgt gaacgtctcg atattctgac catccagaca ggttacccct ttgtatccgc
     2761 caacccagat tcggtgccgc gagtcagcaa acagtgacca gagtgtttcc tcaagcaaac
     2821 cgtctttcgt cccgaaatgt cgggcggctc cggtttttgg gttcagggca aacaagccat
     2881 ttgtggcagt agcaatccag acctgttcat ccggggtttc agcaatacca gaaatccgtc
     2941 cgtctcccag accatgggcc gcagtgtagc tggcaaagag ttcactgcta aatcgaaagg
     3001 ccccgtttga ctgggtgcca aaccagagat tcccttcgta atcttcgagc actgccgtca
     3061 acagatcatt tggaattccg tgagttcgat caaaaatttt aacgccatcg ggatcaaggc
     3121 gattaattcc accgccaaac atcgcacacc agatccggtg atagcggtca atcagaatct
     3181 tgccggcttg agggctattg agcccgtctt tggtggtgta cgtgcgaaat tttgctccgt
     3241 caaaaaagca tgggccgcca ttggtggcaa accagacccc accagccgga tcaatatcaa
     3301 catcaaaggc gctctctccg ggcagtccat cagccagagt aaacaccgtt gccctggtcc
     3361 catcaaattt cccggccccg ccaccacgag tcacaaacca gattatccca gccgaatcct
     3421 gggccaaact ccagaccggc cagttaggca gaccggcttc tttcccaaag tggacaaacc
     3481 gctggccatc aaagtgactg agtccagcag ccgtgccgag ccagagactt ccatcaggtg
     3541 cctcaagccc agagtagacg tgatcatcca caagcccctg ggcagtggtg taattggtaa
     3601 atttcccctg ctcaaaacaa ctcaggccgc gcatggtgca aaaccacagg cgaccttttc
     3661 gatcttcaaa aatggtccga acactgctat cagccagccc ttgctgaaca ccaaaggagg
     3721 tgaagttggc accatcatag cggcaggcgc cgctgtcagt tccaaaccac atatagccct
     3781 ggcgatcctg gtacagacaa taaatcgtgt ttcccggcaa ccccatgctg gcagtgtagg
     3841 acgtaaagcg gtaaatctgc gcaactgtct tgtcaggcga caagagccca atcacaaacg
     3901 cacagatgag cagaagtcca ttccaacgca taaggaaaaa ccagagcaaa atgaagaatg
     3961 aagaaacggg ttccgggttc cgggttctgg gttccgggtt ttcgaaacca agcaccaagc
     4021 accaagtacc aggcactttc ttcattctca attcttcatt cttcattctt cattcttcat
     4081 tcttcattct tc
//
LOCUS       DAOQDO010000251         4091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15201_length_4091_cov_2.740585, whole genome shotgun sequence.
ACCESSION   DAOQDO010000251 DAOQDO010000000
VERSION     DAOQDO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15201_length_4091_cov_2.740585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKZ53_05325"
     CDS             complement(<1..594)
                     /locus_tag="PKZ53_05325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNJ39888.1"
                     /translation="MHEDLISLLPKTEFTRREFVVTSLAAGFALAVQPVSAQTITTDT
                     TGLIAGEVKIPVKDGEMPAYRAMPEKGKAFPVVLVVQEIFGVHEHIRDVCRRFAKAGY
                     LAVAPELYARQGDVSKLSNFDQIIEVVRKVPDSQVMADLDATVAWAKATKKGETKHLG
                     ITGFCWGGRVVWLYSAHNPQVKAGVAWYGRLVGTASPL"
     gene            786..1565
                     /locus_tag="PKZ53_05330"
     CDS             786..1565
                     /locus_tag="PKZ53_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ39889.1"
                     /translation="MSIHHRLKNKVVMITGASSGIGYSTALAFAAEGAHLALGARRCD
                     KMKELARLAHERYDVRVFTHPLDVCSTQSVTAFVEAAVVEFGNIDILVNNAGLALGIA
                     KVSEGSDEDWMTMLDTNVMGVLRVTRAMLPHLTKQAAGHIINLGSLAGHISYPGGSVY
                     AGTKHALRAISEALRQELLGQPIRVTSIDPGLVETEFSQIRFKGDADRAKKVYSGISP
                     LTAEDIAECVIFAASRPTHVNIDTMIVTSIDQAGMNVHRKS"
     gene            1998..>4091
                     /locus_tag="PKZ53_05335"
     CDS             1998..>4091
                     /locus_tag="PKZ53_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ39890.1"
                     /translation="MLCVFFLVVACLGTTFWFLQSPQASAAKSRRTQEVAPDGGLQAW
                     STPEGQSLPKVYEPGQLIVRLKPASQVAPKVRALEFESRLTALQIPVQVVRPAFEDLA
                     ERLATGQTLNDILQTDLARFPKQKARVAAIDPALQVSNLKALAETVVVEVDKETDIEA
                     ACAKLEQSGLCVYAEPNWRAYTFAQPPNDPYYSSRGTWKQSYDDLWGLKNISALDAWD
                     KSTGKDIVIAIVDTGIDYLHPDLAANIWKNTKEIPNNGVDDDRNGFIDDYLGWDFSGG
                     DIDRLDDADNDPRDSFGHGTHVAGIAAGVGNNGIGVVGVAYEAKLMAVKTFPDRLPYG
                     SLLEIARGVEYAAQNGADVINCSLGGPRIRVMEDACALAETKGVVVVVSAGNNRTSTE
                     SVTPAEIGTVVTVAALGPSQNIASFSNFGPKLDVAAPGSDVLSCLAENAAIVESAEGR
                     ERIVTASDGTKYLRLSGTSMAAPHVSGIAALVRSRFPFFTNQQVRQVLKSSTDLSSQP
                     IPQKFSLQLGYGRISASSAIRQLERGNPVAVQLTSPTPYKIYFNSDQIPVSGTATGIE
                     FESYMLQVGVGESPTTWTQLATSLQPAVNKELTTWKVPSDAEGGTYTFRLIGFNRSGG
                     VFIDQASILLIRDKELVEGWPSPLGGYTLSNPAVADLNGDGKMEVVVEGLTVFGDGRI
                     YVMDANGQPFSDAWPK"
BASE COUNT          962 a          998 c         1099 g         1032 t
ORIGIN      
        1 cagcggggag gcggtgccca ccagccggcc ataccaggcc acgccggcct tgacctgtgg
       61 attatgggca ctatagagcc agaccacacg gccaccccag caaaatccag taattccaag
      121 gtgtttggtc tcgccttttt tggtcgcttt ggcccaggcg acagtggcat ccagatcagc
      181 catgacttgt gaatctggta ctttgcggac aacttcgata atctggtcaa agtttgaaag
      241 ttttgagaca tcaccctgac gggcatacag ttcgggggct acggccagat agccagcttt
      301 ggcaaagcgg cggcagacgt cgcggatatg ctcgtgaacg ccaaaaattt cctgaaccac
      361 cagcacgact ggaaaggcct ttcctttttc aggcatggca cggtaggccg gcatttcgcc
      421 atctttgacc gggattttga cttcaccggc aatgagacct gtggtgtcag tggtaatggt
      481 ctgggctgaa accggttgga cggccagggc aaagccggca gccagtgacg tcacgacaaa
      541 ttcacgccgg gtgaactcgg ttttgggaag caaactgatg agatcttcgt gcatagggcc
      601 tgggagtact ccttatagac agatgaaatg gtttggaaaa tgccgggtca ggttgtttct
      661 tgagacctgg atttgtatct tagcaagact ggtttgcaaa gccttcaaag ttgctcgact
      721 acgaatctgt gacaaattac agttttccat attcttcttc tgaaagtatc tgattgaaag
      781 catttatgag tattcatcat cgtttgaaaa acaaagttgt gatgatcacc ggtgccagtt
      841 ccggaattgg ctatagcacg gctctggcat ttgctgctga aggtgcccat ctggcgctcg
      901 gcgcccgccg gtgtgacaaa atgaaagaac tggcgcgtct ggcccacgaa cgctatgacg
      961 tccgggtctt tacccacccg cttgatgtgt gttccaccca aagcgtcact gcttttgtcg
     1021 aagcggcggt ggtcgagttt ggcaacattg atattttggt gaacaacgcc gggctggcgc
     1081 tcggaattgc caaagtgagc gaagggtcag acgaagactg gatgaccatg cttgatacca
     1141 atgtgatggg ggttttgcgg gtgaccagag ccatgttacc ccatctgacc aaacaggcag
     1201 ccggtcacat tatcaatctt ggttcactgg cgggacatat ttcctaccct ggcggcagcg
     1261 tgtatgccgg aaccaagcac gccctgcgcg ccatttctga agccttacga caggaattgc
     1321 tcggccagcc aattcgcgtg acttcgattg atccagggct ggttgaaact gagttcagtc
     1381 aaattcgatt taaaggggat gccgaccggg caaagaaagt ctactcagga atttcgccgc
     1441 tgacggctga agatattgcc gaatgtgtta tctttgcagc ttcccgacca acccacgtca
     1501 atattgacac catgattgtg acttcgattg atcaggctgg aatgaacgtc catcggaaaa
     1561 gctagggggt ttggagtgcg gtggcttgac accgcttttc aggtaaaaac ggtgttaagc
     1621 cgccgcactc caaccatttg ctctttgcat cgaaccctgt tttctggtac agtaacgcga
     1681 cttcaagtaa aaattcacat cttcttattt aaaacactga tcccattgac gatacacttc
     1741 caatccctac cccacactga atgcttggag gattcgacaa ggtttaattt ttttctttgt
     1801 tgacacaact ttgtaaagcc gtcgcacgac aattcctgaa gaaacgaaga aaaggttaat
     1861 taccctcctt cctgcgcata tcatccaggt ttaagcattt tctgcctgcg aggtctattt
     1921 ttttcatgat caccacaatt cagccttctc aacccgcttc tctgagccga cggggccggt
     1981 ccagaaattg gcaccaggtg ctgtgtgtct tttttctggt cgtggcttgt ttgggaacga
     2041 cgttttggtt tttacaatct ccacaagcca gtgcggcaaa aagccggcgg acccaggaag
     2101 ttgcgcccga tggtggcctt caagcctggt ccactccaga aggccagtcg cttccaaaag
     2161 tatatgaacc cggtcaattg attgtccggc tcaagccagc gtcccaggtg gccccgaaag
     2221 tacgggccct ggagtttgaa agtcggttga ccgccctgca gattccagtg caggtcgttc
     2281 gccctgcatt tgaagatctg gccgagcgct tagccaccgg gcaaacgctc aatgacattc
     2341 tgcaaactga tcttgctcga tttccaaaac agaaagcgcg agtggcggcc attgacccgg
     2401 cgctccaggt cagtaacctg aaagccctgg ctgaaaccgt agtggttgaa gttgataagg
     2461 aaaccgacat cgaagccgcc tgtgccaaac ttgagcaaag tggtttgtgt gtatatgccg
     2521 aacccaactg gcgcgcctat acctttgctc agccaccaaa tgatccttat tacagctccc
     2581 gaggcacctg gaagcagagt tacgatgatt tgtggggatt gaaaaacata tctgccctgg
     2641 atgcctggga taaatcgact gggaaagata ttgtgatcgc cattgttgat actgggattg
     2701 attatctgca ccctgatctg gccgccaata tctggaaaaa tactaaggaa attccaaata
     2761 acggagttga tgatgatcgc aatggattta ttgacgatta tttgggatgg gatttttctg
     2821 gtggagatat tgatcggctt gacgatgctg ataacgaccc ccgtgacagt tttgggcatg
     2881 gaactcacgt ggcagggatt gccgccgggg ttggaaacaa tgggattggc gtggtcggcg
     2941 tggcctatga agccaaatta atggcggtca aaacctttcc agaccgtctg ccttatggca
     3001 gtttgttgga aattgccagg ggcgtcgaat acgccgcgca aaatggagct gatgtcatca
     3061 attgttcgct tggtggacca cgcatccggg tgatggaaga tgcctgtgct ctggccgaaa
     3121 cgaaaggtgt ggtagtggtt gtgtcggcag ggaataatcg aacttcaacc gaaagcgtga
     3181 ctcctgccga aattggaacg gtggtgacgg tggcggctct ggggccgagt cagaatatcg
     3241 ccagtttttc aaattttggc cccaaactgg atgttgctgc tcctggttcc gatgttcttt
     3301 cgtgtctggc tgagaatgct gccattgtgg aatcagccga agggcgcgag cggattgtga
     3361 cggcatctga tggtaccaaa taccttcgat taagtgggac aagcatggcg gccccgcatg
     3421 tatctggaat tgcggctctg gtgcgttccc gatttccatt ttttaccaat cagcaggttc
     3481 gccaggtgtt gaaaagttca acggacctca gcagtcagcc catccctcaa aaattttcac
     3541 tccagcttgg gtatgggcga attagcgcct cttcagcgat tcgccagctt gagcgcggca
     3601 acccggtggc ggttcagttg acatcgccga caccgtataa aatttatttt aactcagacc
     3661 agatacctgt ttctggaacc gcgacgggga tcgaatttga gtcctatatg ctccaggttg
     3721 gtgtcggcga atcgcccacc acctggacac aactggcgac atcactccaa ccggctgtca
     3781 ataaagagct taccacctgg aaggtcccca gtgatgccga gggtggtacc tacacatttc
     3841 gattgattgg ttttaaccga tcaggtgggg tttttattga tcaggcatca attctcttga
     3901 tccgagacaa agaattggtc gaaggctggc cgtcgccact cggcgggtac acgctttcaa
     3961 acccggctgt cgctgatctc aatggcgatg gaaaaatgga agttgtcgtt gaaggtttga
     4021 cggtgtttgg tgatgggcgg atttatgtca tggatgccaa tggtcagcct ttttccgatg
     4081 cctggccaaa a
//
LOCUS       DAOQDO010000252         4075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15299_length_4075_cov_3.084080, whole genome shotgun sequence.
ACCESSION   DAOQDO010000252 DAOQDO010000000
VERSION     DAOQDO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15299_length_4075_cov_3.084080"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKZ53_05340"
     CDS             complement(<1..775)
                     /locus_tag="PKZ53_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ39891.1"
                     /translation="MTQCDRFRDQISFYLDDELGEPERAQLTNHLGQCTSCHKLFEQE
                     QQFLAHIRASQPLYQVSPNFRAKIEQALAEVSAPHRAPASLRDRIQKSMDAQPSSASD
                     SFWNWKFLALAASVILVVLLGIWVFDLNSASRGMPTPSEFARMAVDTHLRRLRGQLPL
                     EISTDSPAQISAWFAGKVAFQLKLPNYQESSGQTRLYQIEGARLVGFKNDYAAFVAYQ
                     MQHRPITLVVTANSVVLPSGGEEIKSKGITFHCDSINHLK"
     gene            complement(792..1349)
                     /locus_tag="PKZ53_05345"
     CDS             complement(792..1349)
                     /locus_tag="PKZ53_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ39892.1"
                     /translation="MSSDPLQASQFSQSALEHLDALYGFAMALTHNQTEAEDLVQETY
                     LRAARAFGRLLPDSNLKSWLFAIMRNIWLNQIRHDQSGPQFVELDAEEDSRRDWLDAE
                     HSNPHVRLVQKIEREQVQTAIRQLSEPHREVIILRDIEGFSYQQIASILHCPIGTVMS
                     RLGRAREQLRLLLSPPDLNPSPGER"
     gene            complement(1486..2334)
                     /locus_tag="PKZ53_05350"
     CDS             complement(1486..2334)
                     /locus_tag="PKZ53_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNJ39893.1"
                     /translation="MSFDTITLQCFLAVAETGSFTRAADRVGRTQSAISQQIAKLEEL
                     LGKPLIIRSKTLALTNEGEIFLGYARQIFALHREAMDRFKEPELEGEVRFGLPEDFAS
                     VFLSDVLVDFARIHPRILLNIECDLTLNLFERFKRGGLDLVLVKMNRPEDFPNGLEVW
                     SEKLEWVGDLSLFAHPDQTKPLPLVLSPQPCVYRARAIAALEEAGMKWRLVFSSPSYA
                     GTIAAVKAGLGVTVLPRTMIPEQLHALPRQEFPELSDTHVSLLKHNAGNAVINSFEEF
                     VLKKLR"
     gene            2445..3791
                     /locus_tag="PKZ53_05355"
     CDS             2445..3791
                     /locus_tag="PKZ53_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ39894.1"
                     /translation="MSGYSFESLFHLDALAQVLMALVGFIGLVVWLFARRYMQGDQHY
                     HRFLGLLVLLVFSVMTMAAADHLLFLLFTWGISNALLVCLMVHKAQWKAARASGWVTA
                     RIFLFGFILLSSAFALLFYTTGATSVQTIVKTSSGGTWPVTVALILMGLAAMVQSAIW
                     PFHGWLLSSLNSPTPVSAIMHAGLVNGGGFLLVRFAPLYINQSTLLMGLFVLGIATAL
                     IGTFWKLMQPNIKRMLACSTMGQMGFMLAQCGLGLFPAAVAHLCWHGMFKAFLFLGSG
                     SAAQEKRLPTGAPPDALTFLLALVCGLAGSYGFALASHQTWMAMDTTLVLTAVACIAG
                     SQFALTFLPATPWKRFPLALILTSLMGGLYGSSVWLVEIVLEPLHLMQPQPLNAIHLT
                     GVLALGLVWVGMVFGHTLCQSRILPRTFLKVYVKALNASQPHPDTITTHRNTYRYV"
     gene            3784..>4075
                     /locus_tag="PKZ53_05360"
     CDS             3784..>4075
                     /locus_tag="PKZ53_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNJ39895.1"
                     /translation="MSEVIMQSTFQTLAPESFVSDALNKKIEPPRVQDAIQQAWQAMA
                     PFWPLKNLIACNPLQGLEDLPFEQALIEGAVRFQQATLPAPMEAVNRQTIKWC"
BASE COUNT          922 a         1073 c         1050 g         1030 t
ORIGIN      
        1 ccttcagatg gttgattgag tcacagtgaa aggtaatgcc tttcgatttg atttcttcac
       61 cgcccgatgg cagcaccact gaattggccg ttaccaccag cgtaattggg cgatgttgca
      121 tttggtaagc aacaaatgcg gcataatcat ttttaaaacc aaccagacgc gctccttcaa
      181 tctgatacaa tcgagtttgt cctgaggatt cctgatagtt tggcagtttc aattgaaaag
      241 ccacttttcc agcaaaccag gctgaaatct gtgctggtga atcagtcgag atttcaagcg
      301 gaagctgtcc acgtaatcgc cggagatgag tatccactgc catgcgggca aattcggaag
      361 gcgtcggcat accacgactg gcagagttca ggtcaaaaac ccaaatccca agcagaacga
      421 ccagaatcac tgacgcggcg agcgccagaa atttccaatt ccagaatgaa tctgaagcag
      481 aactgggctg agcgtccatt gatttttgga ttcgatcccg aagtgatgct ggtgcccggt
      541 gcggcgctga aacttcggca agcgcctgtt caattttggc tcgaaagttg gggctgacct
      601 gataaagcgg ctgactggca cgaatgtgtg ccagaaactg ctgctcctgc tcaaacaact
      661 tgtggcatga cgtgcattgc cccagatggt tggtgagttg tgcgcgctct ggttcgccca
      721 gttcatcgtc aagataaaag ctgatttgat ctcgaaatcg gtcacactga gtcatggctg
      781 aaaataccgg attacctttc accaggagat ggattcaggt ctggagggct cagcaacaac
      841 cgaagttgtt ccctggcacg tccaagtcgg gacatcactg tcccaatcgg acagtgcaaa
      901 atgctggcaa tttgttgata actgaaccct tcgatatcac gcaaaatgat gacttcacga
      961 tggggctctg aaagttggcg aatggccgtt tgcacctgtt cgcgttcgat tttctgaacc
     1021 agccgcacgt gcgggttgct gtgttcggcg tcaagccagt cacggcggga atcttcttca
     1081 gcatccagtt ccacaaactg cgggccgctt tgatcgtgac ggatttgatt gagccagata
     1141 ttgcgcataa tggcaaacaa ccagcttttc aaattgctat ccgggagcaa ccgcccaaag
     1201 gcgcgggcag ctcgaaggta cgtctcctgg accagatctt cggcctcagt ctggttgtgg
     1261 gtcaaagcca tggcaaagcc atagagcgca tccaggtgct ctaacgctga ctgactgaac
     1321 tgggacgctt gcaaagggtc tgatgacatt gcgggcctgg ggtgaaagct ttgggctcaa
     1381 gatgttgagt taaagtcgtt tgcactgaac tgagtggcgt gaaaccgtat tctgcgcccg
     1441 aacttcgtcc agtcttccgc ccaacatttt cagtttgaac ctgcatcatc tcaacttctt
     1501 tagaacaaat tcttcaaaag aattgatgac ggcattcccg gcattatgct tcaaaagaga
     1561 cacgtgtgta tcgctcaatt ccggaaattc ctgacgcggc agcgcatgca actgctctgg
     1621 aatcatcgtc cgtggcagaa cggtcacgcc cagacccgct ttgacagccg caatagtccc
     1681 ggcatagctg ggactggaaa aaaccagccg ccacttcatc ccagcttctt ctagtgcggc
     1741 aatggctcgg gcacgataca cacagggttg cggcgagagc accagcggca atggtttggt
     1801 ttgatcaggg tgagcaaaca gactgagatc cccgacccat tcgagttttt ccgaccatac
     1861 ctcaagacca ttgggaaaat cctcgggacg gttcattttc accagaacca ggtcaagccc
     1921 acctcgcttg aagcgctcaa acaggttgag tgtcaggtca cactcaatat tgagcaaaat
     1981 ccgcggatga atccgggcaa aatccaccag gacatctgac agaaaaacgc tggcgaaatc
     2041 ttccggcaac ccaaaccgga cttcaccttc cagctctggc tctttaaagc gatccatcgc
     2101 ttcccggtgg agcgcaaaaa tctggcgggc atatcccaga aaaatctcgc cttcattggt
     2161 aagtgccagg gttttgctcc ggataatcag tggtttgcca agtaattctt caagtttggc
     2221 aatttgctga ctgatggccg attgggtccg tccaacccgg tcagcagccc tggtaaaact
     2281 cccggtttca gccacagcca gaaaacattg aagcgtaatc gtatcaaagc tcatttgttt
     2341 ttctaattct cgatattaaa attattcgtt tcccttatag tagcgatcct tatatcctcg
     2401 ggcaactgaa acaagatttt cgaataactg tgaggaatta tattatgtca gggtattcat
     2461 tcgaatccct tttccacctt gatgcactgg ctcaggtact gatggcactg gttgggttta
     2521 tcggcctggt cgtctggctc tttgcccgac gatatatgca gggagatcaa cactatcatc
     2581 gctttttggg attactggtg ctgctggtgt tttcggttat gaccatggcg gcggctgacc
     2641 acctgttgtt cttgctcttc acctggggga tcagtaacgc gctgctggtg tgcctgatgg
     2701 ttcataaggc ccagtggaag gccgcccgcg cctcaggctg ggtcaccgct cgaatattcc
     2761 tgtttggatt tatcctgctt tcatcggcat ttgcattgct tttttacacc acaggcgcca
     2821 cttcggttca gacaattgtg aaaacctcat ctggcggaac ctggccagtc acggtggctc
     2881 tgatactgat gggactggca gcaatggttc aatcagccat ctggcctttt catggctggc
     2941 tgctcagttc gctgaactct ccgacaccgg tgtcggccat catgcacgcc ggactggtca
     3001 atggcggcgg ctttctgctg gttcgctttg cgccgctcta tataaatcag tcaacgcttc
     3061 tgatgggatt gtttgtcctt ggcatagcaa ctgcactcat tggaacattt tggaagctca
     3121 tgcaacccaa catcaagcgg atgctggcct gttcgacaat ggggcagatg ggctttatgc
     3181 tcgcccaatg cgggctggga ctcttcccgg cagccgtggc gcatctgtgc tggcacggga
     3241 tgtttaaagc ctttttgttt cttggatccg gcagtgccgc tcaggaaaaa cgattgccca
     3301 ctggtgcccc acccgatgcg ctgacttttc ttctggccct ggtctgtgga ttggccggaa
     3361 gttacgggtt tgcgctcgcc agtcaccaga cctggatggc catggatacg accctggtgt
     3421 taaccgcagt ggcctgtatc gctggcagtc agtttgccct gacctttctc ccagccaccc
     3481 cctggaagcg ctttccgctg gcgttgatac tgaccagtct gatgggtggg ctctatggca
     3541 gcagcgtctg gctggttgag atcgtccttg aaccgctcca cctgatgcag cctcagccgt
     3601 tgaatgccat tcatcttaca ggggtgctgg ctttggggtt ggtctgggtg ggaatggtgt
     3661 ttggtcacac cctgtgccag tccaggatcc tgccccgcac gttcctgaag gtgtatgtca
     3721 aagcattgaa tgccagccag ccacatcctg acaccattac cactcaccgc aatacctatc
     3781 gttatgtctg aggtcatcat gcaatctact tttcaaactc tcgcacccga gtcatttgtt
     3841 tctgatgctt tgaacaaaaa aattgaaccc ccacgggttc aggatgccat tcaacaggcc
     3901 tggcaggcga tggcgccgtt ctggccactg aaaaacctga ttgcctgtaa cccattgcag
     3961 gggttagaag accttccgtt tgagcaggca ttgatcgaag gggcggttcg ttttcagcaa
     4021 gccacgctgc ccgccccgat ggaggccgtg aatcggcaga ccatcaaatg gtgcc
//
LOCUS       DAOQDO010000253         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15305_length_4074_cov_4.716845, whole genome shotgun sequence.
ACCESSION   DAOQDO010000253 DAOQDO010000000
VERSION     DAOQDO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15305_length_4074_cov_4.716845"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..3111
                     /locus_tag="PKZ53_05365"
     CDS             355..3111
                     /locus_tag="PKZ53_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNJ39896.1"
                     /translation="MKAKVVSLTQSLILAIGLILIGSQGMVFAQSATGGLRGTVTDES
                     GAVLAGAEIKLKGVDTGAEASTTTSGDGLFRIPALTPGLYNVTVVAAGFKRGEYTSVA
                     IKVGTDITLNVTLQPGGADEIVEVSASDNVVLQRDVSQIATNFESRKVTDLPINTAGG
                     GVDTIAYLTPGVVNPQDAGFTNTNGGGGISSNGGRSRSNNFSIDGQDNNDISVAGPSF
                     AVDNADLVQEFQIITNNFSAEYGQSSGAVVNIVSKGGTNEFHGTLSYFFRDRKNLDSL
                     TNLERRQGLKDPPARITNTFGATFGGPIIKDKLLFFGSYQGIRQAESSLQQSTASGLT
                     PTPAGLQTLLGIADSNIAGVIRAAAPFNIANGNPTIRPDIATRFIPVTVNGRTAQVEF
                     GAISRLVGQPFEENFGTIRLDWNKSEKLRIFGRYFYSKTESANATGNFQNGFFGDVPA
                     RSQQAGGTIVYQISPNAVNETRINYSRIRVFFGGGGNSTIPSASDAGSAVAFFNLPAG
                     FLDWGVATNLPQGRINDNYQVLNNFSYIAGKHSLKMGLDMKRRLTDSFFLPLQNGQFN
                     FTNFNNFSSNVVASGNVAFGKNVVNFPEFDQFYYFQDDWRIRENLTLNLGVRYENSGQ
                     PINVLNDITRERESNPETALFRLDVPLEDRIVRRIDTDKNNFAPRLGFAYSPRFWKKI
                     FGDSNTVFRGGYGIAYDLAFYNILLNVQTSTPTVFLVSVPGGTGVVPSDPTGTSVRGA
                     LQRLAPLRTLDPRLLNRTDVAPDFHAPYTQQYSFGMQRQFGTKTIAEVRYVGTTSIGQ
                     FQSVNANPRIDAIARDFPGFLPSGVRPSPNGRLIAGNGLLRTRINGATSTYHSLQTRF
                     DTQAIKDLNLGVSYTFSKQLDNASEIFGTFAGGGTTAFAQDPFNTTRGEKSFGGFDFR
                     HVLR"
     gene            3153..3767
                     /locus_tag="PKZ53_05370"
     CDS             3153..3767
                     /locus_tag="PKZ53_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39897.1"
                     /translation="MVGKLLGGFQLSGTYQALPGQRYTPVQFFFGSPYNDGGFTAAFN
                     GAAESLRPFYGNPNAPVGTVAIDQQTATDVFGFTSTSPTGYFLLNAFNQNGAEVPVSV
                     NDVRFIANTGATARMFGSPYGNVARNSEKGDRTSVGNFAIFKTTNVTEKMKLQFRAEF
                     FNVFNTPNRGVPDPFIDDAGTTFADINENSGGRRTIQFGLKLIF"
BASE COUNT          948 a         1043 c         1025 g         1058 t
ORIGIN      
        1 agctggttgc ctcttgagaa agagcaagga tactttatta attttaactt aagctatttt
       61 gttgaaaata aaatattttt agtgaatata cttgcaatag atagttatta aacctttgat
      121 ttgcctggat ggtctggcta ctggcgtgaa aattgcttac tcacagggca ttccattttt
      181 ttgaaccccc ctttagcttg ctcaaaacaa tcaaaaagaa aacacccagg ctgtaaacca
      241 gcttctgcct gcgaaatgcc ttgttcctta ccagcatcag tttgtttgga gcaggtttaa
      301 gttggttcct tttttgcagg tcaacaaaca agaattgaca cggagggatg tacaatgaaa
      361 gcaaaagttg tatctttgac acagtccctg attctcgcca tcgggttgat attgatcgga
      421 agccagggaa tggttttcgc ccaatctgca accggcggac ttcggggaac tgtaactgat
      481 gaaagcggtg ccgtgctcgc cggtgccgaa atcaaactca aaggggttga taccggtgct
      541 gaggcgtcaa ccaccacgtc cggtgacggc ttgtttcgca ttccggcctt gactcctggt
      601 ttgtacaacg tgacggtggt tgccgccggc ttcaaacgtg gcgaatatac ctcagttgcc
      661 atcaaagtgg ggactgatat caccttaaat gtcacgcttc agcctggcgg tgccgatgaa
      721 atcgtcgaag tttccgccag tgacaatgtg gtcttgcagc gcgacgtctc gcagattgcc
      781 accaactttg aatcgcgcaa agtgacagat ttgccgatca acacggctgg tggtggggta
      841 gacacgattg cctatcttac cccaggtgtg gtcaatccgc aggatgccgg gtttaccaat
      901 accaacggtg gcggcgggat ttcgtccaat ggtggacggt cacgttccaa caacttcagc
      961 attgacggtc aggataacaa cgacatttcg gtggctggtc catcatttgc cgttgacaat
     1021 gcggatctgg tccaggaatt tcagatcatc accaacaact tctcggctga atatggtcag
     1081 tcatcaggtg cggtcgtcaa cattgtgtcc aaaggtggca ccaatgaatt tcacggcacg
     1141 ctgtcatatt tcttccgtga ccggaagaac ctcgattcat tgaccaacct ggaacgccgc
     1201 cagggattga aagatccccc ggcccggatt accaacacct ttggcgccac gtttggcgga
     1261 ccgattatca aagacaaatt gttgtttttt ggcagctatc agggcattcg gcaggccgaa
     1321 tcttccctgc aacagtccac cgcttctgga ttgaccccga ctccggctgg tctgcaaacc
     1381 ctgttgggaa ttgcggattc caatattgct ggtgtcattc gggccgcggc cccattcaac
     1441 attgccaatg gcaacccgac gattcgtcct gacattgcca cgcgattcat tccagtgacc
     1501 gtcaatggcc ggaccgctca ggttgaattt ggcgccattt ctcgtctggt gggacagccg
     1561 tttgaagaaa actttggcac catccgtctg gattggaaca agagcgaaaa actgcggatc
     1621 tttggccgct atttctattc caagactgaa agcgccaacg cgactggaaa tttccagaat
     1681 gggttcttcg gtgatgttcc ggcccgcagc cagcaggctg gcggtacgat tgtgtatcaa
     1741 atttcaccga atgcggtgaa tgaaacccga atcaactaca gccgcatccg cgtcttcttt
     1801 ggcggtggcg gaaacagcac gattccttcg gcgagtgatg ccggttcggc agtggcgttc
     1861 tttaacctgc cggctggttt cctggattgg ggtgtggcga ccaacctccc ccagggccgt
     1921 atcaatgaca actatcaggt attgaacaat ttctcgtaca tcgccgggaa gcactcgctc
     1981 aagatggggc ttgatatgaa gcgccggttg acagattcgt tcttcctgcc gctgcagaac
     2041 ggtcagttta acttcaccaa tttcaacaac ttctccagca atgttgtggc gtcgggcaac
     2101 gtcgcgtttg gtaaaaacgt ggtgaacttc cctgaattcg accagtttta ctatttccag
     2161 gatgactggc ggattcgtga aaacctgacc ttgaacctgg gtgtacgcta tgaaaatagc
     2221 ggtcagccaa tcaacgtgct caacgacatt acccgtgagc gtgaatcaaa tcccgaaaca
     2281 gcgttgttcc ggctggatgt gccgcttgaa gatcgcattg tgcggcgcat tgacaccgac
     2341 aaaaacaatt ttgctccacg tcttggattt gcctactcac cacggttctg gaaaaagatt
     2401 tttggcgaca gcaacacggt attccgtggc ggatatggga ttgcctatga tctggcgttc
     2461 tacaacatct tgttgaacgt gcagacctca accccgaccg tgtttttggt gagcgtccct
     2521 ggtggcacgg gtgttgttcc gtctgatcca accgggacct cggttcgtgg cgccctgcaa
     2581 cgtctggcac cgctccgcac gctggatcca cgtctgttga accgaaccga tgtggcacct
     2641 gatttccacg ctccgtacac tcagcagtac tcatttggga tgcagcgtca gtttggtacc
     2701 aagacgattg ctgaagtgcg ctatgtcggc acgacgtcaa tcggacagtt ccagtctgtc
     2761 aacgccaacc cacgcattga tgcgattgca cgtgattttc cagggttttt gccctctggt
     2821 gtgcgtccat cgcccaatgg ccggttgatc gctggcaacg gattactccg gacccgtatc
     2881 aacggtgcga cttcaaccta tcactcactg caaacgcggt ttgatactca ggcgatcaag
     2941 gacctgaacc tgggtgtttc gtacaccttc tcaaaacagc tcgacaatgc gtccgaaatt
     3001 ttcggaacgt tcgctggcgg tggaaccacg gcgtttgccc aggatccatt taatacaacg
     3061 cgtggtgaaa agtcgtttgg aggattcgat ttccgccatg ttttgcgctg aacttcctgt
     3121 atgacatccc attcttccgc gaacagcggg gtgtggtcgg caaattgctg ggcgggttcc
     3181 agctttctgg aacctatcag gcactgcctg gccagcgcta tacgccagtt cagttcttct
     3241 ttggcagccc atacaacgat ggtggattta cagccgcctt caatggtgcg gctgaatcgc
     3301 tgcgtccatt ctatggcaat ccaaatgcac ccgttggcac cgtggcaatt gaccagcaga
     3361 ctgccactga tgtgtttggc tttacctcaa cctcgccaac tggttacttc ctgctcaacg
     3421 cctttaacca gaacggcgct gaagttccag tcagcgtcaa tgatgtgcgc tttattgcca
     3481 acaccggtgc cacggcccgg atgtttggta gcccatatgg caatgtggca cgcaactcgg
     3541 aaaaaggtga ccggacttca gttggaaact ttgcgatctt taagaccacc aatgtgactg
     3601 aaaaaatgaa gctgcaattc cgggctgaat tctttaacgt gtttaacacc ccgaatcggg
     3661 gcgtgccgga tccattcatt gatgatgcgg gaacaacctt tgctgatatc aatgaaaaca
     3721 gtggcggacg ccggacgatt cagtttgggt tgaaactgat tttctaaagt tgccaaaaac
     3781 aagagtcctc aatatcttct ctccaggagg tggttctgcc acctcctttt tttgattcag
     3841 ccagtggaat tcagcgtctg agcaatacaa aaaatagccc ttctgccagg gttaagcctg
     3901 ccgtaactgc tttgtctctg aatggatagt tgagctggga aattggtttc gatacttcac
     3961 tcaagttgaa ccccaaccca gaaaatcaac aaatgggctt tgaattctcc tatagaaggt
     4021 ttagagttga attctcctgg aaatatctgg tagggttctg tattcatgtg cgct
//
LOCUS       DAOQDO010000254         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15313_length_4074_cov_2.636726, whole genome shotgun sequence.
ACCESSION   DAOQDO010000254 DAOQDO010000000
VERSION     DAOQDO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15313_length_4074_cov_2.636726"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PKZ53_05375"
     CDS             <1..1522
                     /locus_tag="PKZ53_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ39898.1"
                     /translation="QRITALLADAHSLLIGTQTGGLIAFDGLQFTQVQFESEQRSSPA
                     IECLSTIESRLYVGTYSEGLWVQTGGRWQQFTTSHGLPSDRVVGIAELGEQVIVATDF
                     GLASASKTEMDQTDTTTPLLFHTIATLPGLSAISTTHGHILACQDNGTISLVVKSGNR
                     LSVEPLDQPPVNQTTSLSGCRISAVENSVFLSSDQGLWQSSEITTDVPQLAFEPVALP
                     ASLGSLTSNQVSALEFDADGNLWAGTFRDGIDVLSPTGKQIAHLESDALRDINRLVFD
                     PQLKIMWAATSNGLMKIDALTKTSSVTTRQGLLSNSVNSVFPGTELLVATSDGLSTGK
                     PNQFRSLTRVQGLPGNSVYCVLPVGRSVFVGTGGGLAELQSGKVIRVWTDSNSYLTHN
                     WVSALTATESRLFIGTYGGGVFELLPSGDLHAFDIGKQTVNPNALMCFGNKLGVGTLD
                     GAWMLDLNSQKWTHLTSELPSLTVLSLAANDDHLYVGTDAGIVVLKQGSGFRVQGL"
     gene            1655..>4074
                     /locus_tag="PKZ53_05380"
     CDS             1655..>4074
                     /locus_tag="PKZ53_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HNJ39899.1"
                     /translation="MKHIFKQIYNLKVWNPEPGTRNPEPWLRNPVFLLAVLLILPCWV
                     PVFRSHAQSGVLIPSSIKDKPDAAILSLQVMHVDVRIDNQLARVRVRQIFDNAISSTL
                     EGKYLFALPKLASISDFAVWDDDLRIPGVVMEKRRANEIYTQIKNQQVDPGLLQQDDE
                     HGGNSAFSAKVFPIPPFGTKRLELEYTQMLPVDSLTSHFTFPLKPAFGDVQTCANFSI
                     HVHIVSETPLSPPVFRSKAYPMRALKSSPNEFEYEFTGQQVKLTDDFALDYQLKLQTS
                     SLSWLAYRAPEYISAYDLRDPAQAAQNPDGYFQATATFQMGQASKAPQARNVLLLLDT
                     SLSMHGEKLVRAVEAVEFFLHSLTPQDRFNLLLFHSEAIPFSPQPVPGTPDQIEAALN
                     LIRSSYLSGGTNLKQALVKSKEMAEQFPVGERTLILISDANPTLGTTQQKQLIQIFTF
                     HQKELSGLRFFAFALGSDAKTTWLETLAEQFNGFSVQVRETEDIATQLKLFFDKVGAS
                     ILSGVQFVPADPELLYQVYPNGQHTFDGGSLAFVGRYKPSAGSTSIQVQAYHGTDPIQ
                     LSQTVSLPELDATHDQLPRLWAQARVTALLKEMDLNGEREDYISEIIRLAQKYKLDTP
                     YTAFIAAPRALLRPRLIQPGDPVIRVKTDESITSVFAVLPFGETLPLQFIEDKQVWET
                     RFLAPAWLPDGTYRCRLLLTDKNGNGYQEAKTFVVDSHPPKLKGTADTPTVRSGELVR
                     LRVSADSDTVRLVAKMYGAQPVQLVWSDQEKTNCGTLCIPKGLAAGRYFVTVTAEDFA
                     HNQASTEI"
BASE COUNT          947 a         1117 c         1037 g          973 t
ORIGIN      
        1 tcaacgcatt acggccctgc tggctgacgc tcattccctg ttgattggga cacagaccgg
       61 cggattgatt gcctttgatg gattgcaatt tacccaggtt cagtttgaat ctgagcagcg
      121 ctcatcccct gcgattgaat gcctgtccac aattgaatca cggctctatg tcggcaccta
      181 ttccgaagga ctatgggttc agaccggtgg tcgctggcaa cagttcacca ccagccacgg
      241 tctgccttct gaccgggtgg tgggcatcgc cgaactgggt gaacaggtca ttgtggcgac
      301 cgatttcggg ctggccagtg cctctaaaac cgaaatggat cagacagata caaccacacc
      361 acttctgttc cacacgatag cgactcttcc aggactttcg gcaatcagta ccactcacgg
      421 acacattctg gcctgtcagg acaatggcac gatctcgctg gtagtcaaaa gcggcaaccg
      481 gctttcggtt gaaccacttg atcagccacc tgtcaatcaa acgacttccc tttctggatg
      541 tcgaatttca gcagttgaaa acagcgtgtt tttgagtagt gaccagggtt tgtggcagtc
      601 atcagaaatc accaccgatg ttcctcaact tgcatttgaa ccagttgccc ttccagcctc
      661 gctgggttcg ctgaccagta atcaggtttc ggctctggag tttgacgcag acggaaacct
      721 ctgggccggg accttccgtg acggcattga tgtcctttca ccaacaggaa aacaaattgc
      781 tcatcttgaa tctgatgcgc tccgggatat aaaccggctg gtgtttgacc ctcaactcaa
      841 aatcatgtgg gcggccacgt caaatggact catgaagatt gacgcgctca ccaaaacttc
      901 ttcggtcaca acccggcagg ggttgttaag caattccgtc aattcagtgt ttccaggcac
      961 agaattgctt gtcgccacca gcgatggtct ttccacgggg aagcccaatc aattcagaag
     1021 tttgacccga gttcaaggct tgccgggcaa ttcggtgtat tgcgttctgc cggttggacg
     1081 ctcggtgttt gtcggcaccg gaggcgggct ggccgaattg caatccggca aagtcatccg
     1141 cgtctggacc gattccaatt catacctgac ccataactgg gtgtcagccc tcaccgcgac
     1201 agaatcacgt ttgtttatag gaacgtacgg cggcggggtc tttgaacttc ttccgtccgg
     1261 cgatctgcac gccttcgaca tcggaaaaca aaccgtcaat cccaatgcct tgatgtgttt
     1321 cggaaacaaa cttggcgtcg gcacgctcga cggagcctgg atgctggacc tgaactcaca
     1381 gaaatggaca cacctgacca gcgaacttcc ctctctgaca gtgttaagcc tggcggcgaa
     1441 cgatgaccac ctctatgtgg gaacggatgc cggaattgtt gtcttaaagc agggttcagg
     1501 gttcagggtt cagggtcttt aaaaccgggt tcagggttca gggtctttaa agcaggggtc
     1561 gtgggtcctt tcacgtgtag gtatttggtt ttgcaccgcg aagagttgca aaaccaaaac
     1621 aaactttcac ttactctttg attgaggatc agtaatgaaa catatattca agcaaatcta
     1681 taatttgaag gtttggaacc cggaacccgg aacccggaac ccggaaccct ggcttcggaa
     1741 ccctgtattc ctcctggctg tcctcctcat ccttccctgc tgggttccgg tgtttcgtag
     1801 ccacgctcag tcaggggtac tgattccatc ttcgatcaaa gataaacccg atgccgcgat
     1861 tctttcgctt caggtgatgc acgttgatgt gcggattgac aatcaactgg ctcgggttcg
     1921 ggttcggcag atttttgaca atgcgatttc ctcgacgctt gaaggaaaat acctgtttgc
     1981 gctgccaaaa ctggcttcga tttcggattt tgcggtctgg gacgatgatc tgcggattcc
     2041 cggtgtggtg atggaaaaac gccgggcgaa tgaaatctat actcagatca aaaatcagca
     2101 ggttgatcca ggtctgctcc aacaggacga cgaacacggc ggaaattcag cgttttcagc
     2161 caaagtcttt ccgattccgc catttggcac caaacgactg gagcttgaat acacccaaat
     2221 gctgccggtt gacagcctga catcacattt tacgttccct ctcaaaccgg cctttggcga
     2281 cgttcaaacc tgcgccaact tttccatcca cgtccacatt gtcagcgaaa ccccgctctc
     2341 gccgccggtt tttcggtcaa aggcgtatcc gatgcgggcg ctcaaatcca gtcccaatga
     2401 atttgaatat gagttcacgg gtcagcaggt caaattgacg gatgattttg cgctcgatta
     2461 tcaactgaag cttcaaacca gttcgctgtc gtggctggcc tatcgcgcac cagaatacat
     2521 ttcagcctat gacctgcgcg acccggcgca ggccgcacaa aacccagatg gctattttca
     2581 agccacggcg acgtttcaga tgggtcaggc ttcaaaagca ccccaggcgc gaaatgtttt
     2641 gctgctcctg gacacatcgc tttcgatgca cggggaaaaa ctcgttcggg cagtcgaagc
     2701 cgtggagttc ttcctgcata gcctgacacc gcaagatcgc tttaacctgc tgctttttca
     2761 ttctgaagcc attccctttt caccccaacc ggtcccaggc acacccgacc agatcgaagc
     2821 cgccttgaat ttgattcgaa gctcttacct ttccggcggc accaatctca aacaggcact
     2881 cgtcaagtcg aaggaaatgg ctgagcagtt tccagtgggt gaacgaactc tgatcctgat
     2941 ttcagatgcc aacccaacgc taggcacaac ccagcaaaag caattgatcc agatatttac
     3001 ctttcaccag aaggaactca gcgggctgcg attttttgct tttgcgctgg ggagtgatgc
     3061 caaaacgacg tggcttgaaa ccctggccga gcaattcaac ggcttctcgg tccaggtccg
     3121 ggaaaccgaa gacatcgcca cccaactcaa gctgtttttt gacaaagttg gggcttcgat
     3181 tctgtcggga gtgcagtttg ttcccgccga cccagagttg ttgtatcagg tttacccaaa
     3241 cggtcagcat acgtttgacg gaggcagcct ggcgtttgtc ggacggtata aaccatctgc
     3301 cggctcgact tcgattcagg ttcaggcata tcacggcact gacccgattc aactttccca
     3361 aaccgtttcg ctgcctgagc tggacgccac ccacgatcag ttaccccgat tgtgggccca
     3421 ggccagggta accgccttgc tcaaggaaat ggatttgaat ggcgaacgcg aagactacat
     3481 ttcagaaatc atccggctgg ctcagaaata caagctcgac acgccgtaca cggccttcat
     3541 tgcagcgcca cgggcgttgc tgcggccacg gctgattcaa ccgggcgacc cggtgatccg
     3601 ggtcaaaacg gatgaatcca ttacgtcggt gtttgccgtg ctgccgtttg gcgagacgct
     3661 tcccttgcaa tttattgaag ataaacaggt ttgggaaacc agattcctgg cgccggcgtg
     3721 gttgcccgat ggaacctatc gctgccgatt gctcctgacc gataaaaatg gcaacggcta
     3781 tcaggaagcc aaaacctttg tggttgactc tcatccaccc aagctcaaag gcacggcaga
     3841 cacacccacc gttagaagcg gtgaactggt gcggctccgg gtatcggctg atagcgacac
     3901 ggtgcggctg gttgccaaaa tgtacggtgc ccaacccgtt cagcttgtct ggtcagacca
     3961 ggaaaaaacc aattgtggaa cgctgtgcat tccaaagggt ctggcggctg gacgctactt
     4021 cgtcacggta acggctgaag atttcgccca caatcaggca agtactgaga ttga
//
LOCUS       DAOQDO010000255         4070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15341_length_4070_cov_3.433126, whole genome shotgun sequence.
ACCESSION   DAOQDO010000255 DAOQDO010000000
VERSION     DAOQDO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15341_length_4070_cov_3.433126"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PKZ53_05385"
     CDS             <1..1443
                     /locus_tag="PKZ53_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39900.1"
                     /translation="VFDLTGYHADEANDPGWWASHLHPEDQDRILATESTLETTSHLI
                     VEYRFRRRDGTYFWVRDEKRVLFDSHHQPTEIVGSWSDITERVRLEEQLRQSQKMEAV
                     GQLAGGIAHDFNNLLTVIVGYSDLLLAKMPSTDPKWTNVADIKHAGEQAAVLTRQLLA
                     FSRKQILEPKIIDLNETVSQIEKMLRRLIGEDIILTTILPPSVSKVKVDPGQLEQVII
                     NLAVNARDAMPQGGHLTIETGMTELDEEYCHSRPECTPGWYTQFSLSDTGCGMSPEIR
                     NHIFEPFFTTKEQGKGTGLGLATVFGIVKQSGGHIEVYSEVGVGTSFKIYLPAIEEKW
                     TVMQPETPTEDAQHGNEIILLVEDEAIVRTIARLSLETFGYQVIEAGSGDEALELAKT
                     LTEPLSLLITDVIMPKMNGRQLATQFQHQFPNLKVLLMSGYTDDAIIRHGVIDDTYAF
                     IQKPFTPNSLAKKVREVLEEKNGRSKRTKS"
     gene            1699..4026
                     /locus_tag="PKZ53_05390"
     CDS             1699..4026
                     /locus_tag="PKZ53_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ39901.1"
                     /translation="MDFLYQVFQTQSDPLAFLGLIVGSSFLVVILVMSGGFLFFRNLT
                     QKPHAPEVSEARRKRLHSAISTPPLQLLGFYPVVTLVLIYLVVGATWILVSDQVISSL
                     IRDPELISRLQTYKGWFYVSVTAILLGALTQRYLNQLRWAERELYESQNCLAGIIASA
                     LDGIIIVEEQRGITLLNTAAERLFGRTTAELFGKPLKVLIPDCVQLASDSQSPEKQSA
                     ATQLGNLCLSYGLRASGESFPIEATISRIEVTGRAFFTVILRDITERKRVQEEMQRFI
                     SGSPAVIYALTVEPGHPRTIWVSKNVADITGYTLAEVMADDWWERNVHPEDKARVNVL
                     GAMVHTVGHQISEYRFRRKDGSYFWVRDESQLLRDEQGIPTEIVGSWSDITERIELED
                     QLRQSQKMEAIGKLAGGVAHDFNNLLTVIIGYSDLLLRRMAEYDPKRNYVTDIRQAGE
                     QASSLTRQLLAFSRKQILEPKILDLNEIVTNIEKMLRRLIGEDIVLTSVLGPALGKVK
                     VDPGQIEQVIINLAVNARDAMPEGGRLTIETRMVEFTEDFCRLHPDYNPGWYVLLSLT
                     DTGCGMTADVKNRIFEPFFTTKEQGKGTGLGLATVFGILKQSEGHIEVNSEVGCGTCF
                     NIYLPVIKEQWEPEASNTLELLRPGHESILLVEDEPVVREVTKQSLEAFSYHVIEASS
                     GQEALDLLKTTSEPVALLITDVVMPQMNGRQLAEQLRSKFPDLKVLFMSGYTDDAIVR
                     HGVLDANVAFLQKPFTPTALAKKVQEILERKQHEE"
BASE COUNT         1043 a         1003 c         1062 g          962 t
ORIGIN      
        1 gtgtttgatc tgactggcta tcacgcggat gaggccaacg accctggctg gtgggccagt
       61 caccttcacc cggaagatca ggaccggata ctggcgaccg aatccacgct cgaaaccacc
      121 agccatctga ttgtggagta tcggtttcgc cgccgggatg gcacctattt ttgggtccgt
      181 gatgaaaaac gggttctgtt tgattcgcac catcagccaa ccgaaattgt tggatcctgg
      241 tctgacatca cagagcgggt gcgcctggaa gaacagctcc gccagtcaca gaaaatggag
      301 gccgtcggtc aactggccgg aggcattgcc catgatttca ataatttatt gacggtgatt
      361 gttggctata gcgatttact gctggcaaaa atgccgtcta cggatccaaa gtggaccaat
      421 gtggctgata tcaagcatgc tggcgagcaa gctgctgtgc tcacccggca gttactggcc
      481 tttagccgta aacaaatcct ggagccaaaa atcattgacc tgaatgaaac cgtcagccag
      541 attgagaaaa tgctccggcg gttaatcggc gaggatatta tcctgaccac gatcctgccg
      601 ccatcggtga gcaaagtgaa agttgaccct ggccagcttg aacaggtcat tattaacctg
      661 gcggtcaatg cccgcgatgc catgccacag ggcgggcacc tgacgattga aaccggcatg
      721 acagagcttg atgaggaata ttgccactcg cgaccagaat gcacgcctgg ctggtacacc
      781 cagttttctc tctcagacac cgggtgtgga atgtcaccag agatcagaaa tcacatcttt
      841 gaaccctttt tcaccaccaa ggagcagggc aagggtacgg ggctcgggct ggcaaccgtg
      901 tttggaattg tcaaacaaag cggcggtcac atcgaggtct atagcgaagt gggcgtcggc
      961 accagcttta aaatctacct gccagcaatt gaggaaaaat ggacggtgat gcaaccagaa
     1021 actccgaccg aggacgctca acacgggaat gaaattattc tcctggttga agatgaagcc
     1081 attgtccgta ccattgcccg gctctcgctg gaaacgtttg ggtatcaggt catcgaagcc
     1141 ggaagcgggg acgaagcact tgaactcgcc aaaacattga ccgagcccct ctcccttctg
     1201 atcaccgatg tgattatgcc caaaatgaat gggcgtcagc ttgccactca attccaacac
     1261 caatttccga atttgaaagt gctgttgatg agcgggtata ccgacgatgc cattatccgc
     1321 catggtgtga ttgacgacac gtatgcgttt atccaaaaac cttttacccc aaactcgctg
     1381 gccaaaaagg tgcgtgaagt gttagaagag aaaaatggaa gaagcaaaag aacgaagtcg
     1441 tgaaatggtg acagcaaaga cccattcgga gtgagggtat caggttcagg gtttggggaa
     1501 atacacttct ttcaatgaat tcgctttcct gtcatacaat gcctgggttg caccatggta
     1561 tgattcgata cttgtgagct tgaccaggca accccacact cacttctgga ttgacgactc
     1621 gttccttacc ctcgctcttt ccttactcat tcactccatt gtagaaagct gggattgaac
     1681 ccaatgctga tggtcatcat ggattttctc taccaggtat ttcaaactca atctgaccct
     1741 cttgcattcc tgggattgat tgtgggcagc agctttctgg ttgttatcct ggtgatgtca
     1801 ggagggtttc tcttttttcg caatcttacc caaaaacctc acgccccgga ggtcagtgag
     1861 gcgcgtcgaa agcgattgca ttcggctatt tccacaccgc cgttgcagtt gctgggattt
     1921 tacccggttg tcacactggt gttgatctat ctggtggttg gggcaacctg gatcctggtg
     1981 tctgatcaag tgatttcaag cctgattcgg gacccggaac tcatcagccg gctgcagact
     2041 tacaaaggct ggttttatgt ctcagtgacg gcgatcttgc ttggggccct gacgcaacgc
     2101 tatttgaatc agcttcgctg ggcagaacgg gaactctacg aaagtcaaaa ttgcctggct
     2161 ggcattattg cttcggcact cgatggaatc attattgttg aagaacaacg tggaattacg
     2221 ctgttaaata cagcggctga acgccttttt ggccgcacca cggctgagct ttttggaaaa
     2281 ccactcaaag tactgatccc agattgtgtc caactggcct ctgattcaca gtcgccagag
     2341 aaacaatctg ccgccactca actgggaaat ctgtgcctct cctatggatt gcgggccagt
     2401 ggcgaatctt ttcctatcga ggccacgatt tcccgcattg aagttactgg gcgggccttc
     2461 tttaccgtga ttttacgcga cattaccgaa cgcaaacgag tgcaggaaga gatgcaacgg
     2521 tttatctcgg gcagcccagc cgtgatttat gccttaaccg tcgaacccgg tcacccacgt
     2581 accatctggg tcagtaaaaa tgtcgctgac attaccggat acaccctggc tgaagttatg
     2641 gccgatgatt ggtgggaacg caatgttcac ccagaagata aagcacgggt gaatgtgctc
     2701 ggggcaatgg ttcataccgt cgggcaccag atttcggaat atcggtttcg tcgcaaagac
     2761 ggaagctact tttgggtccg cgatgaatct cagttattgc gggatgagca gggaattcca
     2821 accgaaatcg ttggttcctg gtcagacatc actgagcgga ttgaactcga agaccagtta
     2881 cgccagtcac aaaagatgga agccatcggc aaactggcgg gcggtgtggc gcacgacttc
     2941 aacaatttgc tgacggtcat catcggctat agcgatttgt tgttgcgacg gatggcggag
     3001 tatgacccca aacgcaacta tgtcaccgac atccgacagg ccggagaaca ggcgtcttct
     3061 ctaacccggc agttgcttgc ttttagccgg aagcagattc tggaaccgaa aatcctggat
     3121 ctcaatgaaa tcgtgaccaa tattgaaaaa atgctccgcc ggttgatcgg cgaagatatt
     3181 gtcctgacct cagtgttagg cccggcatta gggaaagtca aagttgaccc aggtcaaatc
     3241 gagcaggtga ttatcaatct ggcggtcaat gcccgggatg cgatgcctga ggggggacgg
     3301 ttgacgattg aaaccaggat ggtcgagttt accgaagact tttgccgcct gcatcctgac
     3361 tataaccctg gctggtatgt tctgctgtct ttaaccgata ccggctgtgg catgacggct
     3421 gacgtcaaaa atcgcatttt tgaaccgttt ttcactacca aggaacaggg caaaggcacc
     3481 ggacttggat tggcgacggt gtttgggatc ctcaaacaaa gcgaaggaca tatcgaagtc
     3541 aacagcgaag ttgggtgtgg cacctgcttc aacatttacc tgccggtcat caaagaacaa
     3601 tgggaacccg aggcgtcaaa taccctggaa ttgctcagac ctggacacga atcaatttta
     3661 ttggtggaag acgaacctgt tgtccgcgaa gtcaccaaac aatcactgga agcctttagt
     3721 taccacgtca tcgaagccag tagcggccag gaagccctcg acctgctcaa aaccacgtct
     3781 gaaccagttg ctttgttgat cactgacgtg gtgatgcctc aaatgaatgg ccggcaactg
     3841 gcagaacaac tccggagcaa atttccagat ttaaaagtcc tgtttatgag cggttacacc
     3901 gatgatgcca tcgtccggca cggggttctc gatgccaatg tggcgttcct ccaaaaacca
     3961 ttcactccga ccgcactggc caagaaggtg caagaaattt tggaacggaa gcagcatgaa
     4021 gaatgaagaa tgaagaatga agaattgaga atgaagaatg aagaattgag
//
LOCUS       DAOQDO010000256         4065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15374_length_4065_cov_2.111471, whole genome shotgun sequence.
ACCESSION   DAOQDO010000256 DAOQDO010000000
VERSION     DAOQDO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15374_length_4065_cov_2.111471"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKZ53_05395"
     CDS             complement(<1..195)
                     /locus_tag="PKZ53_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39902.1"
                     /translation="MNQPPALPVDPNPTLPEDRQLPEAPSFFSRAVFGPTAVFTALAI
                     GSGELLFWPGLSLPNGAGVIW"
     gene            231..1097
                     /locus_tag="PKZ53_05400"
     CDS             231..1097
                     /locus_tag="PKZ53_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39903.1"
                     /translation="MEEFLTRRSGVSADILRLLSLFGQPIFWRRPGTAHAYNEEASVS
                     LSRPSKKLIAQTIPVNRLESSLRATMWALFQTYYDNVTRDHFDCDLEEKSHVLLFFDS
                     GSRELKGFSTVKRYPHTTAGQRVMVIYSGDTIIDRDYWGQPALASEFIKFTILSKLRY
                     CFTPMYWFLISKGYKTYLLLSRNYVEYWPRYDQPTPSWHNQLMHELASARFGDDWKPK
                     LGIVRFETCHGKLKTDVAPIEPALLSQPDIRFFVERNPGHIHGDELCCLGRIGFGLWW
                     KTLVRQVRKHLF"
     gene            1184..2761
                     /locus_tag="PKZ53_05405"
     CDS             1184..2761
                     /locus_tag="PKZ53_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNJ39904.1"
                     /translation="MSSRLLLKMVSPLYHAYLYSRWKRFDRAARHPEQAQARQLTQIL
                     QANQSSTFGKEFRLAEIDSISAFQQRLPIADYELFSPWIDRIASGEPTVLTRAPVQMF
                     ERTSGSTTANKLIPYTQPLLAEFSNATDAWIFDLIRHFPRLKSLRSYWAISPVGKKKE
                     LTAGGIPIGFQDDSEYLLPLLRWAFAQASAVPASVSQMSDITEWQLTTARHLLAAEDL
                     GLISVWSPTFLTGLMRVISDNWSELLAQLPASRRKQLESAWNQSGAINGQTLWPHLHV
                     ISCWQDGASTQFLPMLNAFFPTLPIQGKGLLATEGVVSFPLCGHQGSVLAITSHFLEF
                     LDLDHSNRKPILAHELRPGGQYSPLLTTGGGLYRYHLKDRVTCVGKFAQTPLIRFEGK
                     LDQTSDLCGEKVSAGMVEHILAKVQTEFSLTVHFALLAPVQTDPPHYGLFLESNANPE
                     QLKALESHLENWLRDNAHYAYCRRLNQLGKVCIYPISNGWEQYQARLIAEGTRLGDIK
                     PTYLDRRWGWQETFAQF"
     gene            complement(2859..3644)
                     /locus_tag="PKZ53_05410"
     CDS             complement(2859..3644)
                     /locus_tag="PKZ53_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNJ39905.1"
                     /translation="MPLSSALEFPQFTTEQCAEFQHRGLVHLKNVFTEDEVAVWQAEC
                     QRLLAIPGLIDESNLRTRFRELSTGGKGLGRMDPVCDISPVFAALAQDERVVHIASTL
                     LGEPARMFKNKLLIKMPNMVGYRLHQDYTYWQMEIPADHLLTLQIPIDPMAGLGSPLE
                     IFPGTHHQLLQPPGVIVDPTEDQVSLEDAELLESYPGDVVAFHSLLVHRSRNNQLSHP
                     LFVLYFSYCAGRYGDCSEAYYELSRQFVRTTLPPELRERAYFR"
     gene            complement(3769..>4065)
                     /locus_tag="PKZ53_05415"
     CDS             complement(3769..>4065)
                     /locus_tag="PKZ53_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ39906.1"
                     /translation="VGPMKTFVIQLDQTRCGSVSTIEVGTFLRVKSLFLRPEWQGKGI
                     GLQVMNLMGHLAYQLEKDAFGIFAVAGSAAEKLYTRAGLLPLTQQHEWNKPLSR"
BASE COUNT          993 a         1056 c         1021 g          995 t
ORIGIN      
        1 ccagatcaca ccggcaccgt ttggcagtga caatcctggc cagaacaaca gttcgccaga
       61 tccaatagcc agcgctgtaa aaactgccgt cggaccaaag accgcccgac tgaaaaagga
      121 tggtgcttcc ggtaattgcc ggtcttcagg gagcgttggg tttgggtcca caggcaaagc
      181 aggaggttga ttcataatga tttaactcgg gagcgaagga aattttgagc gtggaagaat
      241 ttttgacgcg gcgctctggt gtcagtgcgg atatactgcg gttgctttcg ctgtttgggc
      301 aaccaatttt ttggaggcgc ccaggaacag cccacgctta caatgaggaa gcttcggtgt
      361 cgttgtctcg tccatcaaaa aaacttatcg cccagaccat tccagtaaac cgacttgagt
      421 catcacttcg agcaacgatg tgggctttgt ttcaaaccta ttatgacaat gtgacccgag
      481 atcattttga ttgtgacctg gaggaaaaaa gccatgtgct gctctttttt gattctgggt
      541 ccagagaact caaagggttt tcgacggtga aacgctatcc gcacaccacc gccgggcaac
      601 gcgtgatggt catttactct ggcgatacca ttattgaccg tgactattgg ggacaaccag
      661 cactggccag cgaattcatc aaattcacga ttctgagcaa gctccggtat tgcttcacgc
      721 cgatgtactg gtttttgatt tccaaaggct ataaaaccta cctgctcctg agtcgaaatt
      781 atgttgagta ctggccccgg tatgatcaac caacgccttc ctggcacaac cagttaatgc
      841 acgaattagc cagtgcacga tttggtgacg actggaagcc aaaactcgga attgttcgct
      901 ttgaaacctg tcacgggaaa ctgaaaaccg atgtcgcccc gattgagccg gccttgctta
      961 gccagccaga catccgcttt tttgtcgaac gcaatcccgg ccatattcac ggcgacgaac
     1021 tgtgctgtct gggtcggatt gggtttggtt tatggtggaa aacgctggta cggcaggtga
     1081 gaaaacacct gttttagggt tcagggttcg gggtttcgaa tttaagtctt ttttgtactt
     1141 ttgtccctta cgtccttttc catcactcgc tggaacgtca taaatgtcat ctcgtctgct
     1201 gctcaaaatg gtatccccgc tctatcacgc gtatctgtat tcccggtgga agcgttttga
     1261 tcgagctgcg cggcatccag aacaggcaca agccaggcag ctcacccaga ttcttcaagc
     1321 aaatcaatca agcaccttcg gaaaagagtt tcggttggcc gaaattgatt cgatttcggc
     1381 atttcaacaa cgacttccga ttgccgacta tgaactgttc tcaccctgga ttgaccggat
     1441 tgcgagcggc gaacccactg tgctgaccag agcacccgtt cagatgtttg agcgaaccag
     1501 cggttcgact acggcgaata aactgattcc ctacactcag ccgttgctgg ccgaattttc
     1561 caacgcaact gatgcctgga tttttgattt gattcgtcac tttccccggt tgaaatcctt
     1621 gcgaagctac tgggcgattt cacccgtggg aaagaaaaaa gaactgacgg ctggtggaat
     1681 tccgattggg tttcaggatg atagcgaata cctgctgcca ttgctgcgct gggcatttgc
     1741 tcaggcgagt gccgtgccgg cttcggtgtc ccagatgtcc gacatcactg aatggcagct
     1801 aactacggcc cggcatctac ttgcggctga ggatcttgga ctgatttcag tctggagtcc
     1861 gacattttta accgggttga tgcgtgtgat ttctgacaac tggtcggaac ttttggctca
     1921 acttccagcc tcacgccgaa agcaactgga atctgcctgg aatcaatctg gggcgatcaa
     1981 tggtcaaacg ctctggccgc atctgcacgt catttcctgc tggcaggacg gggcatcaac
     2041 ccagtttctc ccgatgttga acgcgttttt cccgacactt ccaattcaag gcaaaggctt
     2101 gctggccacc gaaggcgtcg tgtcgtttcc cctgtgcgga caccagggtt ctgttttggc
     2161 aattaccagt cattttctgg agtttcttga tcttgaccac tcaaaccgaa aaccaattct
     2221 ggcgcacgaa cttcgtccag gtgggcaata ttcgccactc ctcacgactg gcggcgggtt
     2281 gtatcgctac catttaaaag atcgggtgac ctgtgtcggg aagtttgcgc aaacaccgct
     2341 cattcgattt gaaggcaaac tcgaccagac cagcgacctg tgcggtgaaa aagtaagcgc
     2401 cgggatggtc gaacacatcc tggcgaaggt acaaactgaa ttctcactga cggttcactt
     2461 tgcattgctg gcaccggttc aaacggatcc gccgcattac ggtctgttcc tggaatcaaa
     2521 tgcaaacccg gaacagctta aagcgctcga atcacacctt gaaaactggc tccgagacaa
     2581 tgcccattat gcctattgcc ggcggctcaa ccagcttggt aaggtttgta tctatccaat
     2641 ttctaacggc tgggaacagt atcaggcgcg attgatcgcc gaaggcactc gcctcggtga
     2701 catcaaacca acgtatctgg accggcgctg gggctggcag gaaacctttg cccaatttta
     2761 gcaaaagaat ttggagtgct atgacttctc atagctttcg tctccaacga caggtcacca
     2821 gttaccaaag cgccgacaag tcggcgcact ccaaatatct atcgaaagta agctcgctcg
     2881 cggagttcgg gaggcagcgt cgtccgcaca aactggcggc tcaactcata gtaggcttca
     2941 gaacagtctc cgtatcgacc agcacaatat gagaaataca ggacaaacag cgggtgggat
     3001 aattgattgt ttcggctgcg gtgaaccaga agcgaatgaa atgccaccac atcgccaggg
     3061 taggattcca gcaattcagc atcttcaagt gaaacctggt cttcggttgg gtcaacgatc
     3121 acaccgggtg gttgcagaag ctgatggtga gtacctggga aaatctcaag cgggcttccc
     3181 aggccagcca tagggtcaat cgggatttgc agggtcaaca aatggtcggc tggaatttcc
     3241 atttgccagt aggtgtaatc ctgatgcagc cgatatccga ccatattcgg catcttgatg
     3301 agcagcttgt ttttaaacat ccgggcgggt tctcccagca acgtcgaagc aatgtgtacc
     3361 acccgttcgt cctgggccaa agcagcaaaa actggagaaa tatcgcagac tggatccatt
     3421 cgccccagcc cctttccacc agtgctcaac tcacgaaatc gcgtccggag attggattca
     3481 tcaatcaacc ctggaatcgc cagcagtcgc tggcattctg cctgccagac agcaacttca
     3541 tcctcggtaa agacattttt taggtgaaca agtccacggt gctggaattc agcgcactgt
     3601 tcggtggtaa attggggaaa ttcaagggca gaagataaag gcatacaaca gatccttttt
     3661 gtttgtggat gcggcgcaat ggccttgaag agttcgattt gcaagctctc aaccgggcgc
     3721 attgaaccat aagcactgct caaaattcca gaatggcggt tgagattctc agcgagagag
     3781 cggcttattc cattcgtgtt gctgagttaa aggcaacaga cccgctctgg tgtagagttt
     3841 ttcagccgct gaaccagcca cggcaaaaat cccgaaggcg tctttctcca gttgatacgc
     3901 cagatggccc atcaaattca tcacctgcag tccaatgcct tttccctgcc actcagggcg
     3961 caaaaacagg ctcttgacgc gcagaaacgt ccccacttca atcgtcgaaa ccgatccgca
     4021 cctggtttga tctaactgaa tcacaaaggt tttcattggc ccgac
//
LOCUS       DAOQDO010000257         4049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15487_length_4049_cov_3.244116, whole genome shotgun sequence.
ACCESSION   DAOQDO010000257 DAOQDO010000000
VERSION     DAOQDO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15487_length_4049_cov_3.244116"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKZ53_05420"
     CDS             complement(<1..218)
                     /locus_tag="PKZ53_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HNJ39907.1"
                     /translation="MTELMPNTSSQLDAVLSSPSDVQDARLWTAIRAAQEKKAFDIVI
                     LGISQVTSFADYFLLCSGSSTRQTEAIAS"
     gene            complement(231..914)
                     /gene="nadD"
                     /locus_tag="PKZ53_05425"
     CDS             complement(231..914)
                     /gene="nadD"
                     /locus_tag="PKZ53_05425"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNJ39908.1"
                     /translation="MGPTPRRIGLLGGTFDPIHYGHLRLAESLIGLFSFDELLFIPTY
                     SPPHKQFEQVTTAYHRHTMTVLATLHSEHSRVSTIELFAPEKPYTVETVGTFRNIYGP
                     DAHIFFLMGADSFGMLHQWYKFQTLIDLCHIIVMSRPGYDLGDAALHRERFGENRVVD
                     FRNGFSLLTISTELTAPNPRIFLTDLVALDVSATEIRAAVSQGKSIAQYVPPMGERYI
                     YAYQLYKEK"
     gene            complement(919..1998)
                     /gene="obgE"
                     /locus_tag="PKZ53_05430"
     CDS             complement(919..1998)
                     /gene="obgE"
                     /locus_tag="PKZ53_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNJ39909.1"
                     /translation="MFIDRAKIYVEGGHGGNGCLAFRREKFVPRGGPSGGDGGNGGSV
                     YLEASDQMNTLLSFRYNPQYKADRGRHGEGSCCHGKSGADMIVKVPIGTQVFDFDTNE
                     LIADLMEPGQRVLVVAGGRGGRGNAQFATSTNRAPRRHEPGYPGEMRYLQLELKILAD
                     VGLLGYPNVGKSTLISVISAARPKIADYPFTTLVPNLGVVQLEDYRSFVVADIPGLIE
                     GAHEGHGLGLQFLRHVERTRLLLHLIDVSEFSGRDPIQDYETIMRELRFYNTEIAERP
                     QIVVATKHDVVQSPERTDALRDFCKARGLEFFVISSVTGEGVKPLMYRVDQKLTELKA
                     KQPAPPSIFQPSVRVEPGVTAVVEE"
     gene            complement(2090..2377)
                     /gene="rpmA"
                     /locus_tag="PKZ53_05435"
     CDS             complement(2090..2377)
                     /gene="rpmA"
                     /locus_tag="PKZ53_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNJ39910.1"
                     /translation="MAHKKGVGSSRNGRDSNAKRLGVKRFGGQFVTGGSILVRQRGTR
                     YKPGNNVGIGSDDTLFACVDGYVKFEDKGRKGKYISVYPTPAVAPQAAAAV"
     gene            complement(2450..2761)
                     /gene="rplU"
                     /locus_tag="PKZ53_05440"
     CDS             complement(2450..2761)
                     /gene="rplU"
                     /locus_tag="PKZ53_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNJ39911.1"
                     /translation="MSYAVVLTGGKQFRVQAGEVVRFPSLAANVGESVEFDQVLVLSN
                     GSEIKIGQPTVTGAAVKGVVVEQGRGPKVIVFKFKRRKQYKRKQGHRQGFTAVRIESI
                     A"
     gene            3148..>4049
                     /locus_tag="PKZ53_05445"
     CDS             3148..>4049
                     /locus_tag="PKZ53_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ39912.1"
                     /translation="MEPKPEITSMVTPQSILGVSAWAQDARSILHAHASHDRPLILIG
                     EPGTGKRFLARQLHHLSHRSHQPFIAIECQTLSPASLRAALLGLSTMGASTTGQGLLE
                     LARGGTLYLGGLVAWPAELSDLLARIDSSSTLLPPFPRPAASFQPHLVTSQSQAHFDV
                     RLVFGLLPVGEADARETCIAQADRLVIPPLRERKADIEMLAEHFLSEFLQTLGREDRS
                     LGTDARRRLLEYEWPGNIKELKSAMLYAVHHLRTKPVSLPDLPPPLGQGEPQENNSSL
                     SELAQQNLSLQDAVEAFEKQLITEA"
BASE COUNT         1012 a         1108 c          995 g          934 t
ORIGIN      
        1 gaagcaatcg cttcggtttg acgggttgaa gagccgctgc acaacaaaaa gtaatcagca
       61 aagctggtca cctgggaaat tcctaaaata acaatgtcaa aggctttctt ttcttgcgct
      121 gcccgaatgg cagtccagag acgcgcatcc tgaacgtcac tgggggaaga aagcaccgca
      181 tccagttgcg aagaggtgtt tggcatcaat tcggtcatgt gatcttgatt ctacttttct
      241 ttgtatagct gataagcgta aatgtagcgt tcccccatcg gtggaacata ttgcgcgata
      301 gactttcctt gactgacggc ggcgcgaatt tccgttgcgg aaacatccaa tgccaccaga
      361 tccgtcagaa aaatccgtgg gttgggggca gtcagttcag ttgaaatcgt taagagcgaa
      421 aaaccatttc gaaaatcaac cacccgattc tcgccaaatc gttcgcgatg gagtgcggca
      481 tctcccaggt catatcctgg acgcgacatc acgatgatgt ggcagaggtc aatgagcgtc
      541 tgaaatttat accattggtg gagcattcca aacgaatcgg cgcccataag aaagaaaata
      601 tgggcatctg gtccatagat gttgcgaaac gtgcctaccg tttcaaccgt gtagggcttt
      661 tccggcgcaa acagttcaat ggttgaaacc cggctgtgct ccgaatggag cgtggccagt
      721 accgtcatcg tgtggcggtg ataggcggtg gtcacctgct caaattgttt gtgaggcggg
      781 ctataggttg gaataaacag caactcatca aaactaaaca gtccgatcaa gctttccgcc
      841 agacgcaaat gaccataatg gatcggatca aatgttcccc caagtagccc aattcggcgc
      901 ggtgttggtc ccatggcctc actcttcgac aaccgcggtg acaccgggtt caaccctgac
      961 cgatggttga aaaattgatg gtggtgcggg ctgtttggct ttcaactccg tcaatttctg
     1021 atcaactcga tacatcagcg gtttgacgcc ttcacccgta accgatgaaa tcacaaagaa
     1081 ttccaatccg cgggctttgc aaaaatcacg cagcgcgtcg gttcgttcag gtgactgcac
     1141 cacgtcatgc ttggtggcca ccacgatctg cggacgctcg gcaatctcag tgttgtaaaa
     1201 gcgcaattcg cgcataatcg tttcataatc ctgtatcgga tcacgacctg aaaactctga
     1261 gacatcaatc aaatgcagca gtaaccgtgt tcgttcgacg tggcgcaaaa actgcaggcc
     1321 aaggccgtgg ccttcgtggg cgccttcaat caagcccgga atatcagcca cgacaaagct
     1381 gcggtaatct tccagttgaa ccacgcccag attcggcacc agggtggtaa acggatagtc
     1441 ggcaattttg ggtcgcgccg cagaaatcac cgaaatcaaa gtggatttcc cgacatttgg
     1501 atatccgagc aagccaacat ccgccaaaat tttgagttca agctgcagat agcgcatctc
     1561 gccagggtat ccaggttcat gccgacgcgg cgctcgattg gttgacgtcg caaactgggc
     1621 attgccgcgt ccgccacgtc caccggcaac caccagcacc cgctggcctg gctccattaa
     1681 atccgctatc agttcattgg tatcaaagtc aaagacctgg gtgccaatcg gtactttgac
     1741 aatcatatcg gcgccgcttt tgccgtggca gcaactgcct tcgccgtgtc ggccacggtc
     1801 agccttgtat tgtgggttat agcgaaatga aagcagggtg ttcatctggt cagaagcttc
     1861 cagatacact gagcctccgt tgccaccatc gcccccggat ggaccgccac gggggacaaa
     1921 tttctcacgt cggaacgcca aacagccgtt tccaccgtga ccgccttcga cgtaaatttt
     1981 agctcgatca ataaacatat agcaaaaaac acaagggcac gggactgctc tggtcagccc
     2041 gtgcccgtga agagtggaaa cctgttcggg acaagatgtg ctggaaaaat tagacagcgg
     2101 cggcagcctg tggcgccacg gctggcgttg gatacacgct gatgtatttt cctttacgtc
     2161 ccttgtcttc gaatttgaca tagccgtcaa cacaagcaaa caacgtgtca tcggaaccga
     2221 tgccaacgtt gttgccgggc ttgtaccgag tcccgcgctg acgcaccaaa atcgaaccac
     2281 cggtcacaaa ctgaccacca aaacgcttta caccgaggcg ttttgcattt gaatcgcgac
     2341 catttcgcga gctaccaaca ccttttttgt gagccataac aaaaacctct ctcaaccaat
     2401 gcgaaatctt atgatgtaaa attgcctgtg agcagcgcca cggatgccat taggcgatgg
     2461 attcaatgcg caccgcggta aatccctggc gatggccttg tttccgcttg tattgcttgc
     2521 ggcgcttgaa tttaaaaacg atgaccttcg ggccgcggcc ctgttcaacc acaaccccct
     2581 tgacggcggc accggtgacg gttggctgtc caattttgat ttctgagcca ttggaaagga
     2641 ccaacacctg gtcaaattcg accgattctc caacattggc tgccagggat ggaaagcgaa
     2701 ccacttcccc tgcctgaaca cgaaactgct tgcctccagt tagaacgact gcgtacgaca
     2761 caaaggactc cctccagggt tttgatcaac cctctcgatg tgatgatatc ttcttctgta
     2821 ttgaagcgaa aaagtgtgct ttttgagaaa aggggcatta tcaaacgaaa aaacgaactt
     2881 gtcaacctca agggacaaat cagaagtttc aaaaactctc tggaaaagcc cgaaattaga
     2941 ctgaaaaatt tactggaacc tttcattgac tcaagcgtaa aggctcttct gaaacttttc
     3001 tttccatttc ggcactaact cccaaacaat ccgtgagaca aaactccagg ccatctttta
     3061 tcgtcccgga ggtgtaattt gactcagttt cctttgtcaa ataagccatt atctactgga
     3121 agtgtcttcc cgatcacata ttcttggatg gaacctaaac ctgagatcac ctcgatggtc
     3181 acgccacaat ccattcttgg ggtcagcgcc tgggcacagg atgcgcgttc gattctgcac
     3241 gcccacgcct cgcatgaccg tcccctgatt ttgatcgggg agccaggcac gggaaaacgc
     3301 tttcttgcac gccaacttca tcatttaagc catcgcagcc atcagccatt tattgccatt
     3361 gaatgccaga ccctgtcacc agcttcactc cgggcagcat tactgggatt gtccacgatg
     3421 ggcgccagca caaccgggca ggggttgctg gaactggcgc gtggcggcac gctctatctc
     3481 ggtggactgg ttgcctggcc ggctgagttg tctgatctac tggcccgaat tgacagttcg
     3541 agcacgctcc ttccgccgtt tccaagaccg gctgccagtt tccagcccca tctggtgaca
     3601 tcccaatccc aggcacattt tgatgtccgc ctggtgtttg gcctgctccc agtgggcgaa
     3661 gctgatgccc gcgaaacctg cattgctcag gcagaccggc tggtcattcc accactccgg
     3721 gaacgcaaag ctgacattga aatgctggct gaacatttcc tgagtgaatt tttgcagacg
     3781 ctgggccggg aggaccgatc cctgggaact gatgcccgac gacggttgct tgagtacgaa
     3841 tggcccggta acatcaaaga actcaaaagc gcgatgctct atgcggtgca tcatctgcga
     3901 accaagccgg tcagtcttcc ggacctgccg ccaccgctcg ggcagggtga accgcaagaa
     3961 aacaattcat ccctgagtga actcgctcaa caaaacttat cacttcagga cgcagttgaa
     4021 gcttttgaaa aacaactcat caccgaggc
//
LOCUS       DAOQDO010000258         4039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15552_length_4039_cov_3.406878, whole genome shotgun sequence.
ACCESSION   DAOQDO010000258 DAOQDO010000000
VERSION     DAOQDO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15552_length_4039_cov_3.406878"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1871
                     /locus_tag="PKZ53_05450"
     CDS             84..1871
                     /locus_tag="PKZ53_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ39913.1"
                     /translation="MATNGSSHPASHHPHVSPFSRLSDMLWEDRSDLLVLLVYTVMTG
                     VLALAVPLAAQALVNTIAANVLTQPLVVLTLIVFAGLVFAGVLRVFQLMLVEALQQRI
                     FVRVALQLAKQLPRIRHSALVEEYAPELVNRFFDVLTVQKAFAKLLLDGLTALLQVSV
                     GLVLMAVYNPLLLVFDSFLVVFALFVVFVLGIGGLRTSIQESVQKYAVAAWLEELARC
                     HSSFKMSGTIQYLVERADNLTMGYIKSRRLHFRVLLRQAIGNFLFQAIANAGILAVGG
                     WLVINRELTLGQLVAAELIVTAVLAAMEKLVKQLDEVYDLLTGLDKVGHITDLAVERV
                     GGDPVPRLAEGATVVCRDVHFSYRDVSPILAGLNLALNPGDRVSLVGASGVGKSTLVS
                     LMCGLNEPSHGTVEINGVDVRVADLESLRRVVGLVGDANDIFEGTIEENIRVGRAYLT
                     HEDIRKALEMAQLTNDLALMPRGLKTELISGGRNLSRGQIQRLLIARATVGTPQLLIL
                     DEAFIGIDEKTKLTILDAIFDQQNKWTIIDISHDPEVVTRSASIYLLANGKIVESGRT
                     EEIISKPDSAFGGLFPLISLTYCRLQQNS"
     gene            2047..3468
                     /locus_tag="PKZ53_05455"
     CDS             2047..3468
                     /locus_tag="PKZ53_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ39914.1"
                     /translation="MAVHSEALLPTQRLSAFTPDIPVQSLGMVATPHSARPLAIVLIM
                     LVIVLAVSLVYVPWQQSVTGTGRIMVFSPMDRPQNIEAQIPARLVEWNVVEGQNVKSG
                     DVIAKLADLDSKFLDPDQPKRLAAQKEALLARREAAEARAAALENQIQSLTDSRDVAI
                     PAAQQKALQARDRLLAAEQSLQAAEQNFTTAKLNVDRVRELHGKGLRSKRDLELVELD
                     YVRSQTEVERAKAALEVARKDTTVGNLDQTKVVNDTAATISGIQASLASVRETIASVN
                     SDLQKLEVDIQNLDRRIQQRIVTAPVDGQVVRLLKVGTGATVKAGDILAVIAPQTTDQ
                     AVELFLSDNDAPLVAVGRHVRLQFAGWPAVQFSGWPSVAVGTFGGKVAVIDAVDDGKS
                     RYRVIIKPDTELIAQGKDEPWPSPRFLRPGAEVTGWVMLDTVSIGFEMWRQFNAFPAT
                     VSREPMDTKGKSTEEKSGGKDDK"
     gene            3570..>4039
                     /locus_tag="PKZ53_05460"
     CDS             3570..>4039
                     /locus_tag="PKZ53_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39915.1"
                     /translation="MRYDDLKMVLPPLAGLIFVVLGAGLIAHPVMAQPGCTEIRTSSE
                     NQTDPPIPGVVTLSDAGTDKSLSARRLLVNVPSVGVIQQPPSTGTAQLPALQLDEVLV
                     QVEANHPKLLGADVERRIASAKRLEKEGAFDPIGEITNEYLRYNSSSSPGKVSEA"
BASE COUNT          947 a         1006 c         1097 g          989 t
ORIGIN      
        1 ccaagaacca agaaccaaga accaacaagt ttcttcattc tcaattctca attctcaatt
       61 cttcatccta taagggatat gctatggcaa caaatggatc ttcacatcca gcctcacacc
      121 atccgcatgt gagccccttt tcccggctga gcgatatgtt gtgggaggat cgctcagatt
      181 tactggtgct tctggtctat accgttatga ccggagtcct ggcactggcc gtccccctgg
      241 cggcccaggc gctggtcaac acgattgccg ccaatgtgtt gactcaaccg ctggtggtac
      301 tcaccctgat tgtgtttgcc ggcctggtct ttgccggggt gttacgtgtt ttccaactca
      361 tgctggtcga agcgctgcaa cagcgcattt ttgttcgagt cgccctccag ttagccaaac
      421 aactccctcg cattcgacat tcagccctgg ttgaagaata tgcccctgaa ctggtaaacc
      481 ggttttttga cgtgttaact gtccaaaaag cctttgccaa gttgctcctt gatggattaa
      541 cggcattgct ccaggtcagt gtcggactgg ttttaatggc cgtttataac ccgttgttgc
      601 tggtatttga ctcattcctg gtggtgtttg ccctgtttgt ggtgtttgtc ctggggattg
      661 gcggtttgcg caccagtatt caggaatcgg ttcagaagta tgccgttgcc gcctggctcg
      721 aagaactcgc ccggtgccat agcagcttta aaatgagcgg cacgatccag tatctggtcg
      781 agcgggctga taacttgacg atgggatata tcaaatcccg acgactccat tttcgggttt
      841 tactgcgtca agccatcgga aattttctgt tccaggccat tgccaatgcc ggcattctgg
      901 cggttggtgg ctggctggtc atcaatcgcg aattgacact gggtcaactg gtggcagctg
      961 aattgatcgt cacggcagtg ctcgctgcca tggaaaagct ggtcaaacaa cttgatgaag
     1021 tatatgacct gctcaccggg cttgataagg tcggacacat tacagattta gccgttgaac
     1081 gggttggcgg tgatcccgtg ccccgattgg ctgaaggggc cactgtggtg tgccgggatg
     1141 ttcacttttc ctatcgggat gtcagcccaa ttcttgctgg cttaaacctg gctttaaacc
     1201 caggtgaccg ggtgagtctg gtcggtgcca gtggagtcgg aaaatcaacg cttgtgtctt
     1261 tgatgtgtgg attaaatgaa ccaagtcacg gcactgttga aatcaacggg gttgatgtcc
     1321 gggtcgctga cctggaaagt ctgcgtcggg ttgtcgggct ggttggcgat gccaatgata
     1381 ttttcgaagg gacaattgag gaaaacatcc gtgttggccg ggcttatctc acgcatgaag
     1441 acatccggaa agcactggaa atggctcaat tgaccaatga tctggcgtta atgccccgag
     1501 ggctcaaaac cgaactgatc agtggtgggc gcaatctttc cagagggcag attcaacgcc
     1561 tgttgattgc ccgtgctacg gtgggcaccc cacagttact cattctggat gaagcgttta
     1621 tcggaattga tgagaagacc aagttgacaa tcctggatgc gatttttgac cagcagaaca
     1681 aatggaccat tattgatatt tcacacgatc cggaagtggt cactcgttcg gcttcgatct
     1741 atttgctggc caatggaaaa atcgtcgaaa gtggtcgaac cgaagaaatc atttccaaac
     1801 ctgacagtgc ctttggtgga ctattcccct tgatttcatt gacctattgc cgattgcaac
     1861 aaaattcctg attgggtgat ggggtcaacc aaagaccaaa aggacaaacg ggacgaaaag
     1921 gacaaaagga tgaaagagac ctggggaata ttcgttcaca gcccaacaag tttttttgat
     1981 tgaatgactt aacttgactt gaaaacccgg aacccggaac ccggaacccg gaacccgaaa
     2041 aaaattatgg ctgtacattc agaagcactt ttacctactc aacgtctgtc cgcttttaca
     2101 cctgatattc cggtgcaatc actcgggatg gttgctacgc cgcactcagc ccgcccactt
     2161 gccattgttc tgatcatgct agtgatcgtt ctggcggtga gcctggtata tgtgccgtgg
     2221 cagcagtcag tcactggaac tggccggatt atggtgtttt caccgatgga ccgccctcaa
     2281 aatattgagg ctcagattcc agcccgactg gtagagtgga atgtagttga aggccagaat
     2341 gtgaaatctg gtgatgtgat cgccaaactg gctgaccttg attcaaagtt tctggatcca
     2401 gatcaaccca aacgtctggc ggcccaaaag gaagcgctcc tggcacgccg tgaggcggct
     2461 gaagcgcggg cggcggccct cgaaaaccag atccaatctc tgactgactc gcgtgacgtc
     2521 gccattccgg ctgcccagca aaaagcattg caggcacgtg accggttgct ggcggcggaa
     2581 caatcactcc aggcggcgga acagaatttc accaccgcca aactgaatgt tgatcgggtg
     2641 cgggagttgc atggaaaagg attgcggtcc aaacgtgacc tggaactggt tgaactggac
     2701 tatgtccgat ctcagaccga ggtcgagcgc gccaaagccg cgcttgaagt cgcccgtaaa
     2761 gacaccaccg tgggcaatct cgatcaaacc aaagttgtga atgacacggc agcaaccatc
     2821 agtggcatcc aggcgtcact ggcgtctgtg cgcgaaacca ttgcctcggt caatagcgac
     2881 ttacaaaagc tcgaagtgga tattcaaaat ctggaccgac gaattcagca gcgcattgtc
     2941 accgcacccg ttgatgggca ggtggtccga ttattgaagg ttggtactgg tgcaacggtg
     3001 aaggcgggag acattctggc cgtgattgcg ccacagacca ctgatcaggc ggttgagttg
     3061 tttttgtctg acaatgatgc cccgctggtg gcggttggtc gtcatgtccg gttgcagttt
     3121 gcaggctggc ccgcagtcca gttttcaggc tggccatcag ttgccgtcgg gacatttggt
     3181 gggaaagtgg cggtcattga tgccgtggat gatggaaaaa gccgctatcg ggtgattatc
     3241 aagcccgata ccgaactcat cgcccagggc aaggacgaac cctggccgtc accacgtttt
     3301 ctccgacctg gggccgaagt cactggctgg gtgatgctcg ataccgtttc gattggcttt
     3361 gaaatgtggc gacaatttaa tgccttcccg gcaacggtca gccgtgagcc aatggataca
     3421 aagggcaagt caactgagga aaaaagcgga gggaaagacg acaaatgaac ctccatcgcc
     3481 agaaaatggc cttattttat ttagggttca gggttcaggg ttcagggttc agggaagttc
     3541 aatgcttcca attatttcac ttattgcgta tgcgatatga tgatttgaaa atggtgttgc
     3601 cgcccttggc cggacttatc ttcgttgtgc ttggggctgg cctgatcgcg cacccggtta
     3661 tggcgcaacc aggttgtacc gaaatacgca ccagctcaga aaaccagacc gaccctccaa
     3721 tcccaggcgt ggtgactctt tcagatgccg ggacggataa atcactgtct gcacggcgtc
     3781 tactggtcaa tgtaccgagc gttggtgtca ttcagcagcc accttccacc ggtaccgctc
     3841 aactaccggc gctccaactc gatgaagtgc tggttcaggt tgaagccaat cacccgaaat
     3901 tgctgggggc ggatgtcgaa cgtcgcattg cttcggccaa acgtcttgaa aaagaaggtg
     3961 cctttgaccc aattggggaa atcaccaatg aatacctgcg ctataacagt tcatcgagtc
     4021 ccggcaaagt cagtgaagc
//
LOCUS       DAOQDO010000259         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15571_length_4036_cov_3.484049, whole genome shotgun sequence.
ACCESSION   DAOQDO010000259 DAOQDO010000000
VERSION     DAOQDO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15571_length_4036_cov_3.484049"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2015)
                     /gene="glmS"
                     /locus_tag="PKZ53_05465"
     CDS             complement(159..2015)
                     /gene="glmS"
                     /locus_tag="PKZ53_05465"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNJ39916.1"
                     /translation="MCGIVGYVGEKHVVPVLLDGLRKLEYRGYDSAGIAVVDHGNLFV
                     RRAPGKLRNLETNLKLQPIDGTYGVGHTRWATHGRPSEQNAHPHRDASGRIVVVHNGV
                     IENHAHLKQQLLNEGHTFVTQTDTEVIAHLIGKLRDQVESLEDAVRKAVSEFQGIYAI
                     AVMSADEPDKIVTARLGPPIVIGLGNNEYFIASDIPAILEHTRDMFFLADQEMAVLTR
                     SGVLVTDFEGTPQSPNIHRIMWDAHMVEKGGFRHYMLKEIYEQPRSIRDTLLGRVSVD
                     EGHVYLDNLDFRPDEWREFSSIKIAACGTSWHAAMVGKFLIEELARIPVEVDYASEFR
                     YRDPLLDQSTLTVVVTQSGETADTVAALREAKSKNSRVIAVCNVLGSMATREADATVL
                     TQAGPEISVASTKAFTSQVTALYLLALYLAQERGTLDLDVLRFHIEQLLQLPMKMESV
                     LNLDTQIDELGKVIFRSQDFLYLGRGIFFPIALEGALKLKEISYIHAEGYPAGEMKHG
                     PNALIDDRLPVVFITPHETGSRTSEVRYEKTLSNMQEVKAREGIVIAVVTEGDSHAAE
                     LADYVIEIPAACDTISAILAVVPLQLLAYHIAIRRGCDVDQPRNLAKSVTVE"
     gene            complement(2229..3401)
                     /locus_tag="PKZ53_05470"
     CDS             complement(2229..3401)
                     /locus_tag="PKZ53_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015196822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ39917.1"
                     /translation="MPRIYLSVPTMGGRELGYIQQAFDTNWISTVGPNITAFEADFSD
                     RIGQSCVALSSGTAAIQLGLRLLGVGPGDEVFCPTLTFVATANPVLYLGATPVFLDSE
                     TRSWNLDPQLLEEALCERAKINRLPKAVIVVHLFGQAADMDPILEVCQKYDVPILEDA
                     AEAVGTLYRGKPAGTFGDVAAFSFNGNKIMTTSGGGMLTAKDPALVEKARFWSTQARD
                     PGIAYEHSEMGYNFRMSNILAGIGRGQLEIVDELIDCRRRVAFRYQWAFQDVPGIEFM
                     PQAEYGFHTNWLSCFLIDEDQFGCSRDEVIDLLARDDIESRPIWKPMHLQPLYARAER
                     FGGDVAEYLFRRGICLPSSPNLTIEDQHRVIDIIQSAAKRNVQSGAQVETGELIAT"
     gene            complement(3492..>4036)
                     /locus_tag="PKZ53_05475"
     CDS             complement(3492..>4036)
                     /locus_tag="PKZ53_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39918.1"
                     /translation="DFSWLEQPSASVAALALGIGTPSVRLRIGQELAARYPHLEWPTL
                     VHPTGLYDAETCQLGRGVLWFANVLGTVNLQIGDFSMISNGCTIGHESKIGKGCLLNP
                     SVNISGGVLIGDGVLVGVGAQVLQYLRIEAGATIGAGAVVTKSVPEGIPVVGVPAYPL
                     SKPQPSLSRFRPLTASSLSP"
BASE COUNT         1020 a         1058 c         1011 g          947 t
ORIGIN      
        1 gaagggaaaa tagttccact tagggttcag ggttcaggtt tcggggtttg taaaaacaaa
       61 aaggacctaa aggacgaaaa cgacggaaag gacctaagtt cgaaaacccg gaacccggaa
      121 cccggaaccc ggaagcgcgg aacccggaac cctaaaccct attcaacagt tactgacttg
      181 gccagattgc gtggctggtc aacgtcgcac ccgcgccgga tagcaatgtg atacgccagc
      241 aactggagcg gcaccaccgc caggatggcc gaaatcgtgt cacaggctgc cggaatttcg
      301 attacataat cagccagttc ggcagcgtgt gaatcgcctt cggtcaccac ggcaatcaca
      361 atgccttccc tggctttgac ttcctgcata ttcgacaggg ttttttcata tcgtacttct
      421 gaagtccgtg aacctgtctc atggggcgtg ataaacacca cgggcaaccg gtcgtcaatc
      481 aaggcattgg gaccgtgttt catttctcct gccggatagc cttcggcgtg gatgtagcta
      541 atttccttga gcttcagagc accttccagc gcaattggaa aaaagattcc ccgccccaga
      601 tacaaaaaat cctggcttcg gaaaataact tttcccaatt cgtcaatctg ggtgtccagg
      661 ttgaggactg attccatttt catcggcaac tggagcaatt gctcgatgtg gaagcggagg
      721 acgtccagat cgagggtgcc gcgttcctgg gctaaataga gtgccagtaa atacagagcg
      781 gtcacctgcg aagtaaaggc cttggttgac gctactgaaa tctcaggtcc tgcctgagtt
      841 aagacagtgg catccgcttc acgggtcgcc attgatccga gcacattaca cacggcaatc
      901 acccgactgt ttttggattt ggcttcacgg agcgcagcca ccgtgtcagc cgtttcgcca
      961 gactgggtga ccacaaccgt cagggttgac tggtcaagca atggatcccg atagcgaaac
     1021 tcgctcgcat aatcaacttc gaccggaatc cgggcgagtt cttcgatcaa aaacttgcca
     1081 accatcgccg cgtgccagct tgtgccgcag gccgcaattt tgatactgga aaattcccgc
     1141 cactcatcag gtcgaaaatc aaggttatca agatacacat ggccttcgtc aaccgagacc
     1201 cgacccagca acgtgtcgcg gatcgagcgt ggctgctcat aaatttcctt gagcatgtaa
     1261 tgccgaaacc cgcctttttc aaccatgtgg gcgtcccaca taatccggtg gatattggga
     1321 gactgtggcg tgccttcaaa atcggtcacc agaacgcccg accgggttag aaccgccatt
     1381 tcctggtcag ccaggaaaaa catatcgcgg gtatgctcta aaatcgccgg aatatcagag
     1441 gcaataaaat attcgttgtt tcccaagcca atgacaattg gtgggccaag tcgggccgtt
     1501 acaattttgt cgggttcatc cgccgacatc accgcaatcg cataaattcc ctgaaattca
     1561 ctgaccgctt tgcgaacggc gtcttccaat gattcaacct gatcgcggag tttgccaatc
     1621 aaatgggcaa ttacttcggt atcggtttgc gttacaaatg tatggccttc gttcaggagt
     1681 tgctgtttta agtgagcatg attttcaatc acgccattgt gaaccaccac aattcgcccg
     1741 ctcgcatcgc ggtggggatg ggcgttttgc tcagatggcc taccatgggt tgcccaacgg
     1801 gtatgcccga caccataggt tccatcaata ggttggagtt tcagattggt ttcgaggttt
     1861 cttaactttc caggtgcccg ccggacaaac aggtttccgt ggtcaacgac agcaattccg
     1921 gctgaatcat atccacgata ttcgagtttt ctgaggccat ccagcaacac tggaacgacg
     1981 tgcttttcac caacatagcc cacaattcca cacataccga tactccctgg gttcttctgt
     2041 tcaatgaagt ggattttctt ttctggtgaa agagattgtt tggagactgg caccttatca
     2101 cttttttctt ttcaggcaac agaattccat aaaagatcta tttttccagt gagatacaag
     2161 aaaaagaagg tttattttta atacctattt tgacgattga caaaggacag tttgagcgcc
     2221 tgccaggatc aggtggcaat gagttcaccg gtctcaacct gagccccaga ttgtacattt
     2281 cgtttggcgg cggactggat tatatcaatg acccggtgct ggtcttcaat cgtcaaattt
     2341 ggagagctag gcaaacaaat cccacgacga aacagatatt cggcgacgtc accgccaaag
     2401 cgttcggccc gggcatagag tggctgaagg tgcattggtt tccagattgg gcgagactca
     2461 atatcgtcac gtgccagcaa atcaatgact tcatcccggc tgcacccaaa ctggtcttca
     2521 tcaatcaaga aacaactgag ccagttggta tgaaacccat actcagcctg aggcataaat
     2581 tcaattcccg gtacatcctg aaacgcccat tggtatcgga aagccacccg ccgtcggcag
     2641 tcaatcagtt catcaacgat ttccaattgg ccgcgaccaa tgcccgccaa aatgttgctc
     2701 atccgaaaat tgtaccccat ttcggaatgt tcataggcaa tgcctggatc acgggcctga
     2761 gtggaccaga agcgggcttt ttcaaccagg gcaggatctt tggctgtgag catccccccg
     2821 ccgctggtgg tcataatttt gtttccatta aaggaaaatg cggcaacgtc tccgaaggtt
     2881 ccggcaggtt ttccccggta gagcgttcca accgcttcag ccgcatcctc taaaatcgga
     2941 acgtcatatt tctgacacac ctccaaaatc ggatccatat cagcggcctg tccaaacagg
     3001 tggacaacga tcacagcctt gggcagacgg ttgattttcg cccgttcaca gagtgcttct
     3061 tcaagaagtt gtggatccag gttccagctt cgggtttcac tatccaaaaa aacgggggta
     3121 gctcccagat acagcaccgg gttggccgtg gccacaaacg tcagggttgg gcagaaaact
     3181 tcgtctccgg gcccaacacc aagtaaccgt aagcctaact ggatggcagc cgtcccacta
     3241 gagagtgcaa cacacgattg cccgatccgg tcggaaaaat cagcttcaaa ggcggtgata
     3301 tttggaccga cggttgagat ccagttggtg tcaaaagctt gctggatata gccaagttct
     3361 cggccaccca ttgttggaac ggaaagataa atgcggggca tacgactcat ccttattttt
     3421 aaagtgtgcg tgtcagcaca cagggccttg atttctctga aagcgggggg cgtcaggtga
     3481 aatctggctt tttaaggact gagtgaactg gcggtgagtg gtcgaaatcg ggaaagtgat
     3541 ggctgaggtt ttgagagagg ataggcgggg acgccaacga cggggatccc ttcaggtacg
     3601 gatttcgtca cgacggctcc tgctccaatc gtggcgcccg cctctattct gagatactgc
     3661 aatacctgtg ccccaactcc gacaaggacg ccatcaccaa tcaaaacacc gccggaaatg
     3721 ttgaccgagg ggtttaaaag gcagcctttt ccgatttttg actcgtggcc aatcgtgcag
     3781 ccattgctga tcattgaaaa atcaccaatc tgcaaattta ctgttcccaa tacattagca
     3841 aaccagagga ccccacgccc caattgacac gtctcggcat catagagccc ggttgggtgg
     3901 accagggtcg gccactccag gtgtgggtat cgggcagcca gttcctgtcc aattcggagt
     3961 cgaactgacg gagttccgat gccaagtgcc aaggcagcga cacttgcgga aggttgctca
     4021 agccaggaga aatcac
//
LOCUS       DAOQDO010000260         4016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15700_length_4016_cov_3.274931, whole genome shotgun sequence.
ACCESSION   DAOQDO010000260 DAOQDO010000000
VERSION     DAOQDO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15700_length_4016_cov_3.274931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKZ53_05480"
     CDS             complement(<1..741)
                     /locus_tag="PKZ53_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ39919.1"
                     /translation="MKVRSGGGWAAIGYTLEKSFRAFGGPRQFWQRMLSRNSCKTCAV
                     GMGGQAGGMRNEAGHFPEFCKKSVQAMAHDMQPPIDVNFFLSHPVDELAGYSPRQLEN
                     LGRLAYPVIRETGKKNYKRIEWNDALDRIAAAMKATTPDRTFFYSSGRSSNEAAFLLQ
                     WLARVYGTNNVNNCSFYCHQASGVGLADAVGSGTATVTLDDVEHCDFVMLLGANPASN
                     HPRLITQLSDMRKRGGKVVVVNPLLETGL"
     gene            complement(982..2670)
                     /locus_tag="PKZ53_05485"
     CDS             complement(982..2670)
                     /locus_tag="PKZ53_05485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNJ39920.1"
                     /translation="MRLFCLILIFLFSGTQTLLAAQKPAEVVFLNGNIYTLSEAQAQA
                     EALVVQNGKLVFVGSAKAARRYIGKSTKVVDLQGKTVVPGFTDAHCHLAGVGQREMTL
                     NLEGTTSLEGLLATIKQRVDRAKPGEWIIGRGWIETFWTPPVFPTCSDLDRVSPNNPV
                     FLQRADGHGAVANSLVLKMAGITKETPNPAGGEIMRDPKTGEANGMLLDKANFLIVPY
                     VPHPTAEDAVKALLLGVERSLSLGWCQVQIAGNSRAEIDLLNTLYRDGKIKLRIYNAI
                     AGPGPEADELVKSGPQIGDYDHHFTLRGIKLVLDGALGSKGAALLEPYADYNTTGLLT
                     LEEKDVMPLLKEALRRGIQIETHAIGDRANRTILDWYEKAFQAVPPKDRKIRDPRWRV
                     EHAQILNPADIPRFKKLGVIPSMQPSHAIGDLHFAPSRIGIKRLAGAYAWKSLLKTGV
                     VIAGGSDAPVERGEPMIEFYAAVARKDQKGFSGDGWHPEEAVTREEALKMLTLWPAYA
                     AFEEKLRGSLEPGKLADFVVLSADIMRIPEPEILKTQCLMTVVGGEVVFENKVR"
     gene            2680..2805
                     /locus_tag="PKZ53_05490"
     CDS             2680..2805
                     /locus_tag="PKZ53_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39921.1"
                     /translation="MPHHTDLVHREIQLKWIEYVRISAADFIELGNKWQAEQMTQ"
     gene            complement(2927..>4016)
                     /locus_tag="PKZ53_05495"
     CDS             complement(2927..>4016)
                     /locus_tag="PKZ53_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39922.1"
                     /translation="VLQDRIAEKIVEGLKLTISSAELERIHRDVAKNVVAYELFLKAK
                     NYSHDVHEDQTALDLLKSSLEADPTYAPAWAELAMRHLHIALKGLGGITHVVEARKAA
                     NRALEINPDLPEALSALMGVSAEMGDVSEAVRICYRLLEIAPNNELTYLGLGHAYEYS
                     GLLDQALRAYTRSMQINPKLSASYTQIAAVLSQRGRYDLAYEFLTTRDCKEVMCEVKA
                     YLLGVLSMRKGARDEAIRQFKSAEQWSPRSLWGRLSLAMHLNLENDLEGCLNILDQIR
                     ATVTSGDSCFYLAHHYTVLNQPEIVLDLLEKAIDRGYCNVEGYEQDPAFKDFRDLPRF
                     KALVSRARDLQSQFMSCMNELQRKEVAG"
BASE COUNT          963 a         1091 c          963 g          999 t
ORIGIN      
        1 cagaccggtt tccagcagcg gattgaccac cacgaccttt ccaccacgct ttcgcatgtc
       61 agacaactgg gtaatcaacc gggggtggtt actggccgga tttgccccca gcaacattac
      121 aaaatcacaa tgttcgacat catccagtgt cacggttgcc gttcctgaac caaccgcatc
      181 cgccagacca accccgcttg cctgatggca ataaaaagag caattgttga cattgtttgt
      241 gccgtaaact cttgccagcc attgcaataa gaaagctgct tcgttgctgc tgcgtcccga
      301 tgaatagaaa aaagttcgat ccggggtggt ggccttcatg gcagcggcaa tccggtccag
      361 cgcgtcattc cattcaattc gcttgtaatt ttttttcccg gtttcccgaa tcaccggata
      421 tgccagtcgg cccagatttt cgagctgtcg tggactgtat ccggccagtt catccactgg
      481 atgagagaga aagaaattca catcaatcgg tggctgcata tcgtgcgcca tggcctgaac
      541 cgactttttg caaaattctg gaaaatgccc ggcttcattg cgcataccgc ccgcctgacc
      601 gcccatgcca accgcgcagg ttttgcaact attgcgtgaa agcatgcgct gccagaactg
      661 gcggggtccg ccaaaggccc gaaatgattt ttccagggtg taacctatcg ccgcccatcc
      721 accacctgac cgaactttca cagcaaggct ccttcaattt tggtctttac cgattttcag
      781 aacgagaaac cacggaatac acagaaatac acggaaaata aatctttttt tctactcctt
      841 tgctactcca cgatagcttt tcagataaat atttcctgag tatttgtttt gggaagtcgc
      901 agaaacaatt gaaaaatttg gatttagttt ctgtgtattt ctgtgtattc cgtggttttt
      961 tatctgaaat tctatacttg actacctgac tttattttca aatacgactt cccccccaac
     1021 caccgtcatc agacactggg ttttgaggat ttcaggttct ggaatcctca taatatcagc
     1081 cgaaagcacc acaaaatcag cgagtttccc tggttcaagc gagcctctca gtttttcttc
     1141 aaaagcggca taggctggcc agagcgtcaa cattttgagg gcttcttcac gggtgacagc
     1201 ttcttcaggg tgccacccgt cacctgaaaa ccctttttga tctttccggg ccacggcggc
     1261 ataaaattca atcatcggtt cgccgcgttc aaccggcgca tctgacccac cagcaatcac
     1321 aaccccggtt ttgagcaggc ttttccaggc ataggcacca gccagacgct tgattccgat
     1381 tcggctgggt gcaaaatgaa gatcgccaat ggcatgcgac ggctgcattg atggaatcac
     1441 gcccagtttc ttaaagcgtg ggatatcagc cgggttgaga atctgggcat gttcgacccg
     1501 ccagcgagga tcccggattt ttcggtcctt cggtgggacg gcctgaaagg ctttttcata
     1561 ccagtccaga atggttcgat tggcccggtc gccaatcgca tgagtctcaa tctgaatgcc
     1621 ccgccgtagc gcctctttta acaacggcat tacgtctttt tcttccaacg ttaagagtcc
     1681 ggtcgtgttg tagtcggcat acggctcaag caaggcggcg ccttttgagc ctaacgcccc
     1741 atcaagcacc agtttgatcc cgcgcagggt aaaatgatga tcatagtcac caatttgggg
     1801 gccagatttg accagttcgt ctgcttccgg tccgggtccg gcaatggcgt tatagatccg
     1861 gagcttgatt ttcccgtccc ggtacaatgt gttgagtaaa tcaatttcgg cccgcgagtt
     1921 tccggcgatt tggacctggc accaccccaa cgaaaggctg cgctcaacac cgagaagcag
     1981 cgctttaact gcatcttccg cagttggatg cggtacatag ggcactatca ggaagttcgc
     2041 tttatccagc aacatcccat tggcttcccc ggtttttggg tcgcgcataa tttcgccgcc
     2101 agccgggttt ggcgtttctt tggtgatccc cgccattttt agaaccagac tgttggcaac
     2161 cgctccgtga ccgtcagccc gctgcaaaaa cactggatta ttgggtgaga cccgatccaa
     2221 atctgagcag gttggaaaaa ccggtggcgt ccaaaaggtt tcgatccacc cacgtccgat
     2281 gatccactct ccgggtttgg ctcgatcaac ccgctgtttg atggtggcaa gcaacccctc
     2341 caggctggtc gtcccttcca ggttgagcgt catttcacgc tgcccaactc cggccagatg
     2401 gcagtgggca tcggtaaaac ccggcacaac tgttttccct tgtaaatcaa ccactttcgt
     2461 tgattttcca atataccgcc gggccgcttt agctgacccg acgaacacca gttttccatt
     2521 ctggacaacc agcgcctctg cctgggcctg ggcctctgac agggtataaa tattgccatt
     2581 gagaaaaacc acttcggctg gtttttgtgc cgccagaagg gtttgggttc cagagaaaag
     2641 aaaaatcaga atcaggcaaa aaagtctcat acaaataaca tgccgcacca tactgacctg
     2701 gtgcatcgcg agattcaatt gaagtggatt gaatatgtaa ggataagtgc ggctgacttt
     2761 attgaactgg ggaacaaatg gcaagccgaa cagatgacac agtgacaaat gaccaggtga
     2821 gcaatgacaa agtgaccagt gacagggtga gaagcagcag acatcagcgc caggatgagg
     2881 aacatcatcc gcttgcgctc tgaaggagcc atcagaagcc aatctgtcac ccggccactt
     2941 ctttgcgttg aagctcattc atacaggaca taaactggct ctgaagatca cgagcccggc
     3001 tcaccaaagc cttgaaacgg ggcagatcac ggaagtcttt aaacgctgga tcctgttcat
     3061 acccctcaac attgcaatat ccccgatcaa ttgctttttc taacaggtcg agtacgatct
     3121 caggctggtt cagcaccgtg tagtggtgtg ccaggtagaa gcaactgtct ccgctggtca
     3181 ccgtggcccg gatctggtcc agaatgttga gacaaccttc aagatcattt tccaggttga
     3241 ggtgcatcgc cagactgagc ctgccccaga ggctacgcgg actccactgt tcagccgatt
     3301 taaactggcg aatggcctca tccctggcgc ctttgcgcat agaaagcacc ccaagtaaat
     3361 aggctttgac ctcacacata acttccttgc aatcacgggt ggtgaggaat tcataagcca
     3421 ggtcataccg gccacgctgg ctcaaaacgg ctgcaatttg ggtgtacgaa gcacttaatt
     3481 tcgggttgat ttgcatggac cgggtgtagg cccgcaacgc ctgatcgaga agacccgaat
     3541 attcataggc atggcctaac cccagatatg tcaattcgtt atttggagca atctccagca
     3601 atcggtagca aattcgaacg gcttcagaga catcacccat ttcagcgctg acccccatca
     3661 acgccgagag tgcttccggc aggtcaggat tgatttcaag tgcccgattg gctgcttttc
     3721 gggcttcgac cacgtgggta atcccgccta atccttttaa tgcaatatgc aggtggcgca
     3781 tcgccagttc ggcccaggct ggggcatagg ttggatctgc ctcaagcgaa ctcttgagca
     3841 aatcaagtgc cgtctggtcc tcatgtacat cgtggctgta gtttttggct tttaagaaca
     3901 actcatacgc cacgacattt ttggcaacat cgcgatgaat gcgctccagt tcagccgatg
     3961 aaatggtcag tttcaatcct tcgacgattt tctcggcaat tcgatcctgt aacacc
//
LOCUS       DAOQDO010000261         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15712_length_4014_cov_3.061632, whole genome shotgun sequence.
ACCESSION   DAOQDO010000261 DAOQDO010000000
VERSION     DAOQDO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15712_length_4014_cov_3.061632"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..152
                     /locus_tag="PKZ53_05500"
     CDS             12..152
                     /locus_tag="PKZ53_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39923.1"
                     /translation="MLVTRRGMRLSVQPVTKEEWQVIESLEHEMKETGLGAEENGLKK
                     TG"
     gene            210..2768
                     /locus_tag="PKZ53_05505"
     CDS             210..2768
                     /locus_tag="PKZ53_05505"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNJ39924.1"
                     /translation="MSSAPSPEPDVFSPKPDVFSPKPYNPMVTVKMTTVSKRTIFFRW
                     LKRLAIGVLALMVLSGMMGGIFYYRWFQSPLPQLDGEVRLAGLSAPATIARDEFGVPH
                     IQGANQEDLAVAQGYAVAQDRLWQMDLLRRAARGELAEIFGEERLAYDQAQRRLGYVL
                     VAQQALEKLTPEMRRSLDAFARGVNACMTQHRDRLPAEFRMLRYEPRPWEALDSLVIG
                     KLMAQSLNTSFPSDLMRETFRARFDAQTCNRLFGDINPLDRILVGSDTAPPSSPNGKP
                     VSVPEKKEDPKLAQVQTAPELDDSILEREALALVGLDGDGLGSNNWVIGGGRTTTGKP
                     ILCNDTHLGHSVPSIWYLVHLMTPDGAFNVTGFVFPGIPGVIIGHNARIAWGVTNFGP
                     DVQDLFVETFNPARPDEYRVGESWEKASLRTERIQVRTSFWSTQTREEVLQVTTTRHG
                     PIVREENGKRYALRWTALDPLTEFPTFDLLSTASNWQDFQAALKLYPGPMQNFIYADV
                     DGNIGYWAAGMVPIRKSGDGSVPVDGASGEGEWTGYLPFEKLPHLFNPAEGYIVTANQ
                     RITGKSIPEFYTHQWYAPYRAAAIQRRILMKEQHTPDSMNTIHADTWSYPDDLFVKEA
                     LKFFPNHKNEEIWQRIHQDLSEWNGELRADSKVAAIVTTWRSRFIKKLLEAKLGDLEA
                     KYRWENAETVYASLITERPADWLPKKFPSYDELLVATYREAITELEQKFGKYRTSWSY
                     GDLNTLEFAHPLARISLLKPMLNPSVLTMNGSANTVNAYGRGKVWGVSMRQVVDLSNL
                     DNSRQNITVGESGQPASPHYQDQVADWYRVQPHVFAFTSASVENHARHRLILKP"
     gene            2937..>4014
                     /locus_tag="PKZ53_05510"
     CDS             2937..>4014
                     /locus_tag="PKZ53_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNJ39925.1"
                     /translation="MVQAINIRQIRSHFQEILQQLISLRIHRSSANHLFAMIHGYPKQ
                     PTSTTEKGQAMKPTPATNLVLRLLLGIVMAIGAVTPVFAQAQSTTGLIQGEITDTSGA
                     VVAGAKVTAQNESTGLERSVTTNASGLFNLPLLPVGTYKLTVEASGFSTTVQEDVVLT
                     IGQTLTLNLKLQPGGASEVITVTTEPPVVDTGRPEQSTLIDERSVQALPINRRDFTNF
                     IGLTPGVSVVQGPDGDVATLYGQRGTQNNLSIDGADANNPFFGEQRGGQRPAFTIGLE
                     SVKEFQVVPIGASAEFGRSSGGFINVITKSGTNDFRGTAFLYFRNQSIASQNPDAVKA
                     GVPTQDFDNYQFGGNIGGPLKRDKA"
BASE COUNT          977 a         1044 c         1059 g          934 t
ORIGIN      
        1 ggctcgaaaa tatgcttgtc acccggaggg ggatgcgact ctcagtccaa cccgtcacaa
       61 aagaagagtg gcaggtgatt gaatcattgg aacacgagat gaaagaaaca gggcttgggg
      121 ctgaagaaaa cgggctgaag aaaacgggct gaagaaaacg ggtaaaacca atctcacgtc
      181 acagtcttca gccccaagcc ctgagcccga tgtcttcagc cccaagccct gagcccgatg
      241 tcttcagccc taagcccgat gtcttcagcc ccaagcccta taacccaatg gtcactgtca
      301 aaatgacaac tgtttcaaag cgtacgattt ttttccgatg gctcaagcgt ctggcgattg
      361 gggtgctggc tctgatggtg ctgagcggca tgatgggcgg gattttctac tatcgctggt
      421 ttcagtcgcc gttgccgcaa ctcgatggtg aagtcagact ggcaggactt tctgcgccgg
      481 caaccattgc ccgtgatgaa tttggggtgc cccacattca aggtgccaat caggaagatc
      541 tggccgtggc tcaaggatat gcggttgccc aggatcgcct gtggcagatg gatttactcc
      601 gccgggctgc ccgcggagaa ctggcagaaa tttttggtga agagcgtctg gcttatgatc
      661 aggcccagcg acgtttgggc tatgtgctgg tagctcagca ggcgcttgaa aaactcacgc
      721 cagaaatgcg gcgcagcctg gatgcctttg cccgtggagt caatgcctgt atgacccaac
      781 atcgggaccg tctcccggct gagtttcgga tgttgcgcta tgaaccacgt ccctgggaag
      841 cacttgattc actcgtgatt ggcaaactca tggcgcaatc actgaacaca agttttccga
      901 gtgacctgat gcgtgaaaca tttcgagccc gatttgatgc ccaaacctgt aaccggcttt
      961 ttggagatat caaccccctt gaccgcattc tggttggttc agataccgca ccgccgtctt
     1021 cgccaaatgg aaagccggtt tcagttccag agaaaaaaga agaccccaaa ctggctcagg
     1081 ttcagacagc gcctgagttg gatgactcca tcctcgaacg cgaagcactg gcactggtcg
     1141 ggttggacgg tgatgggctc ggcagtaaca actgggtgat tggtggcggg cgtaccacga
     1201 ctggaaaacc gattttgtgc aacgacaccc atctgggcca cagtgtgccc tcgatctggt
     1261 atctggtgca tttgatgaca cctgatggtg cctttaatgt caccgggttt gtctttcctg
     1321 gtattccggg tgtcatcatt ggtcacaatg cccgcattgc ctggggcgtc accaattttg
     1381 gtccggatgt tcaggatttg tttgttgaaa cctttaaccc agcacgtcct gacgaatatc
     1441 gggttggtga atcctgggaa aaagccagtc tccgaactga acgcatccag gtccgcacat
     1501 cattttggtc tacgcagacc cgtgaagaag tgctgcaggt gacaacgacc cgccatgggc
     1561 cgattgtgcg tgaggagaat gggaaacggt acgcgctcag gtggacggcc cttgacccgc
     1621 tcactgagtt tcccacattt gatctccttt caaccgcttc caactggcag gattttcaag
     1681 cggcactcaa gctgtatcct ggcccaatgc aaaatttcat ctatgctgac gttgatggca
     1741 acattgggta ctgggcggcg ggaatggtac cgattcggaa gtcaggtgat ggctcagtcc
     1801 ccgtggatgg tgccagtggg gaaggcgagt ggacaggcta tcttccattt gaaaagcttc
     1861 cacatttgtt caatccagcc gaagggtaca tcgtaacggc aaatcagcgg atcaccggca
     1921 agtcaatccc cgagttttac actcaccagt ggtatgcacc ctatcgagcc gccgcgattc
     1981 agcgccgaat tttgatgaag gaacaacata cgcccgacag catgaacacc atccacgctg
     2041 atacctggtc gtatccggat gatctctttg tcaaagaggc gctgaaattc ttcccaaatc
     2101 acaaaaatga agaaatctgg caacgcatcc accaggattt atccgagtgg aatggggaac
     2161 tccgggctga ttcaaaggtg gctgcgattg tgacgacctg gcggtcccgg ttcataaaga
     2221 aattgttgga ggccaaactt ggtgatctcg aagccaaata ccggtgggaa aatgctgaaa
     2281 ccgtctatgc ctcgcttatt acagagcggc ctgcggattg gctgcccaaa aaatttccga
     2341 gttatgatga gttgctggtt gcaacctacc gcgaagcgat cactgaactg gagcagaaat
     2401 tcggaaaata tcgcacaagc tggagttatg gcgacctcaa cacccttgag tttgctcatc
     2461 cgctggctcg gatttccctc ttaaaaccga tgctgaaccc atcagtgctc accatgaatg
     2521 gttctgccaa cacggtaaac gcctatggcc gtggaaaagt gtggggagtc tcgatgcggc
     2581 aggtggttga tctctcaaac ctggacaact cgcggcaaaa cataacggtt ggggaatcgg
     2641 gtcaaccagc gagcccgcat taccaggatc aggttgccga ctggtatcgc gtacaacccc
     2701 atgtttttgc ctttacctcc gcttcggttg aaaaccacgc ccgccaccgg ttgatcttaa
     2761 agccatgact tgtgtggagt gcggtggctt gacgccgctt ttcattttct gaaaggctat
     2821 agttctagcc cttcgcttca cacccactca ctgatcacta tttggatagt gttcttgaga
     2881 agtgttctgg gaaaatgatt gaaatacctg aaaaaacttg attggcggtt gtctaaatgg
     2941 tacaagccat taacattcgc cagatacgct cgcatttcca ggaaatcctt caacaattga
     3001 tttcacttcg gattcaccga agctcggcaa accatctgtt tgccatgatc cacggctatc
     3061 caaaacaacc aacttcaact acagaaaagg gacaagccat gaaacccacg cctgcaacca
     3121 atctcgtttt gcggctacta ttgggcattg tgatggcaat tggggcagta acgcccgttt
     3181 ttgctcaagc acaatcaacc acaggtttga ttcagggtga gattactgat acctctggag
     3241 ctgtggtggc tggtgcaaag gttacggcgc aaaatgaaag caccggactg gagcgctcgg
     3301 tcacgacgaa tgccagcggg ttatttaatc ttccactgct tcctgtggga acatacaaac
     3361 ttactgttga agcctcagga ttttcgacaa cagttcagga agatgtcgtg ctgaccattg
     3421 gtcagacact gaccctcaat ttgaaactcc aacccggtgg ggcatctgaa gtcatcacgg
     3481 tcaccacaga accacctgtg gttgacaccg ggcgacccga acagagcacc ctgattgatg
     3541 aacgttcagt ccaggcgctg ccaatcaatc gccgcgactt caccaatttc atcgggctga
     3601 cgcctggcgt gtcagttgtg cagggaccgg acggtgacgt tgccaccctg tacggtcagc
     3661 gtggaacgca aaacaacctg tcaattgacg gggctgatgc caataaccca ttttttggtg
     3721 agcagcgcgg cggtcaacgc ccggcgttta caattgggtt ggaatcagtc aaagaattcc
     3781 aggtggtccc gattggagca tcggctgaat ttggccggtc atcgggtggg tttatcaatg
     3841 tgatcaccaa atcagggacc aatgactttc gcggaaccgc cttcctgtat ttccgcaacc
     3901 agtcaattgc cagccagaac ccagatgcgg tgaaagctgg ggtccccacc caggattttg
     3961 ataactatca atttggcggc aacattggcg gcccactcaa acgcgacaaa gcgt
//
LOCUS       DAOQDO010000262         4007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15754_length_4007_cov_3.373229, whole genome shotgun sequence.
ACCESSION   DAOQDO010000262 DAOQDO010000000
VERSION     DAOQDO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15754_length_4007_cov_3.373229"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..3375)
                     /gene="carB"
                     /locus_tag="PKZ53_05515"
     CDS             complement(142..3375)
                     /gene="carB"
                     /locus_tag="PKZ53_05515"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNJ39926.1"
                     /translation="MPKRTDIQKILIIGSGPIVIGQACEFDYSGTQACKALKAEGFTV
                     VLVNSNPATIMTDPGLADQTYIEPLTVEALESIIDRERPDALLPTVGGQTALNLTVRL
                     AEKGILDRYQVTLIGAKIESIHMAENRRLFKQAMDEIGLEMPKAAFVTSLEQIDEVIQ
                     KIGYPAIIRPSFTLGGAGGGVAYNDEEFRQIVTRGLALSPITEVLVEESILGWKEYEL
                     EVMRDHADNIVIVCSIENFDPMGIHTGDSITVAPAQTLTDREYQILRDASLQIIRKIG
                     VETGGSNIQFAVNPHDGRLRVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IPNDITLKTPASFEPALDYVVVKIPRWAFEKFRATQAVLGTQMKSVGEAMAIGRTFKE
                     AFLKALRSLESGKTTALEYVDDQHLRNQLVTPNPDRVGYLMMALSRGWSCETLHQLTS
                     IDPWFLFQLQQLSEIEKEVQSQTLATLSDQRLFELKQLGFADRHLADLLGIEELSVRE
                     RRLASGIRPVFKRVDTCAAEFASFTPYLYSTYEDECEAEPTDQPKVMILGSGPNRIGQ
                     GIEFDYCCCHASFALKEAGYQAIMVNCNPETVSTDYDTADRLYFEPVTFEAVMNIVDL
                     EKPTGVIVQFGGQTPLNLARRLMAAGVPIIGTSPDSIDLAEDRERFGKLLADLNIPQP
                     SNGMALSIEEAIVIANRIGYPVLVRPSYVLGGRAMMIVYDQAGLEQYMTEAVEVSAGK
                     PVLIDRFLESAFEVDVDALCDGEKVCIAGIQEHIEEAGIHSGDSSSVLPPYMIEPWHL
                     DQMRRYTRQLALALKVVGLMNIQFAVKDDTVYVLEVNPRASRTVPFVSKATGVPLAKI
                     AALLMVGKKLTDFNLPPVLSVNRFYIKAPVFPFIKFPGVDPVLGPEMRSIGEVMGVAD
                     SFGMAFLKSQYGAGINLPQRGTAFLSINDQDKPNAIKVAQRLHNLGFKLIATRGTQEM
                     LSKAGLPVEMVFKVNEGRPHIVDIIKSRKVDLIVNTPLGKMSYFDEQAIRSAAIQYEV
                     PCITTITGAVAVTSAIQALQFEAIEVQSLQEYHRSGLDVVLGLKASG"
     gene            3693..>4007
                     /locus_tag="PKZ53_05520"
     CDS             3693..>4007
                     /locus_tag="PKZ53_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39927.1"
                     /translation="MFTLKSNHSRYFALAWLVASCAMTACVIPPGGYFGTIVAPKEQK
                     IVVGNHSEPRSLDAHKTTGVAESNIIKELFEGLTVYHPETLEPLPGSAVSWESQQDAQ
                     VWI"
BASE COUNT          983 a         1074 c          998 g          952 t
ORIGIN      
        1 cctcggcgca ggagacaaac gattctccgg cgttgggatt gtggtcaaaa tagtcgcggg
       61 gcgtgcctcc cgacatcttc agcccgtctt cttcagcccg acgccttcag ccccaagccc
      121 ccagccctta gcccaaaatc ttcagcccga tgccttcagc cccaaaacaa cgtccagccc
      181 tgagcggtga tattcctgca aactctggac ttcaatggct tcaaattgaa gcgcctggat
      241 ggcgctggtg accgcaaccg ctccggtaat ggtggtaatg cagggcactt catattgaat
      301 ggcggcgctg cgaatggcct gttcatcaaa gtaggacatt ttgccaagtg gtgtattgac
      361 aatcagatcc accttacgac tcttgatgat gtccacaatg tgaggacggc cttcgttgac
      421 tttaaagacc atttcaaccg gcaatccagc ctttgaaagc atttcctggg tgccacgcgt
      481 ggcaatcaat ttgaacccaa ggttatgcag ccgctgggcg actttgatgg cattgggttt
      541 atcctggtca ttgatgctca gaaatgcggt tccacgctgc ggcaggttga tccccgcccc
      601 atattgcgat ttcaaaaacg ccattccaaa gctatcggct acccccatca cttccccaat
      661 tgagcgcatt tccggcccca agaccggatc cacaccggga aatttaatga atggaaacac
      721 cggagccttg atgtaaaacc ggttgacaga gagcaccggt ggcaaattga aatcggttaa
      781 cttcttgccc accatcaata acgccgcaat tttggcgagc ggaacacctg tggccttgct
      841 cacaaacggc acggttcgcg aagcacgcgg attgacttcg aggacataga cggtgtcatc
      901 cttgactgca aactggatat tcatcagccc gaccaccttg agcgccaggg ccagttgccg
      961 ggtatagcgc cgcatctggt ccagatgcca gggttcgatc atataaggtg gcaagacact
     1021 tgaactatcg ccagagtgga tgccagcttc ttcgatatgt tcctggatgc ccgcaataca
     1081 caccttttcg ccgtcacaca gcgcgtccac gtcaacttcg aaagctgatt ccaaaaatcg
     1141 gtcaatcaac acgggttttc cagcagaaac ttcgacggct tcggtcatat attgttcgag
     1201 cccggcctga tcatagacaa tcatcatggc acgtccgcca agaacataac ttggacgcac
     1261 cagcaccggg tagccaatcc gattggcaat cacaatcgct tcttcaatgg acagcgccat
     1321 gccattggaa ggctggggaa tattgagatc agccagcagt ttcccaaacc gctcgcgatc
     1381 ttccgccaga tcaatcgaat ctggcgaggt tccaataatc ggcacgccgg cagccattaa
     1441 tcgtcgggcc agattgagtg gcgtctgacc accaaactga acgatcactc cggttggttt
     1501 ttcaaggtca acgatgttca tcaccgcttc aaaggtcacg ggctcaaaat acaaccggtc
     1561 tgccgtgtca tagtcggtcg aaacggtttc tggattacag ttgaccataa tggcttgata
     1621 cccggcttct ttgagggcaa agctggcgtg gcagcaacag taatcaaact caatcccctg
     1681 cccgattcga ttggggccgc tgcctaaaat catgactttc ggctggtcag tgggctcggc
     1741 ttcgcattca tcttcatagg ttgaatagag atagggcgta aacgaagcaa attcagccgc
     1801 gcaggtatcc acccgtttga agaccggtct gatgccagac gccaggcggc gctcgcgaac
     1861 cgatagctcc tcaattccaa gcaaatctgc caggtggcgg tcagcaaacc caagttgttt
     1921 cagctcaaac aatcgctgat ctgaaagggt ggccagcgtt tgagactgaa cctctttttc
     1981 gatttcggaa agttgctgca actggaacaa aaaccaggga tcaattgaag tcaactggtg
     2041 caatgtttca caggaccagc cacgactgag cgccatcatc aaatatccga cgcgatccgg
     2101 gttgggtgtg accagttgat tgcgcaggtg ctggtcatca acatattcaa gcgccgtggt
     2161 ttttccagat tcaagtgacc gaagggcttt gagaaaggct tctttgaacg ttcgaccaat
     2221 cgccatggcc tcaccgacgg atttcatctg ggtgccaagc acggcctggg tcgcacggaa
     2281 cttttcaaac gcccatcgtg gaattttgac gaccacataa tccagcgccg gttcaaaact
     2341 ggctggagtt ttgagagtaa tatcattggg gatttcatcc agcgtgtagc caaccgccag
     2401 tttggccgcc actttggcaa tcgggaaccc ggtggctttt gaggctaagg ctgaagaccg
     2461 tgacacgcgt gggttcatct caatcacgcg caatcgtccg tcgtgtggat tgacagcaaa
     2521 ctggatattg gatccaccag tctcaacccc aatctttcgg ataatctgta atgacgcatc
     2581 gcgcaaaatc tgatattcac gatccgtcag tgtttgcgcc ggggccaccg taatcgagtc
     2641 accggtgtga attcccatcg ggtcaaagtt ttcaattgaa cacacaatca caatgttgtc
     2701 ggcgtggtcg cgcatgactt cgagttcata ctctttccag cccaggattg attcttccac
     2761 cagtacttcg gtaattggac taagtgctaa cccgcgcgtc acaatctggc ggaattcttc
     2821 atcgttatag gcgaccccgc ccccagcccc gccaagcgtg aaactcggtc gaataatggc
     2881 cggataccca atcttttgaa tgacttcgtc aatttgctca agtgaagtca caaaggcggc
     2941 tttgggcatt tcaagaccga tttcatccat tgcctgttta aaaagccgcc gattttcagc
     3001 catgtggata gattcgattt tggcgccaat caaagtgacc tgatagcggt ccagaattcc
     3061 tttctcagcc agtcgaacgg tcaggttgag cgccgtctgg ccgccaaccg tcggcaaaag
     3121 ggcatcgggt cgctcgcggt caataattga ttcgagggct tcgaccgtca gtggttcaat
     3181 ataagtttgg tcagccagtc ccggatcggt cataatcgta gccgggttcg aattgaccag
     3241 aacaacagta aacccttcgg ctttcaaagc tttacaggct tgagtccccg aatagtcaaa
     3301 ttcacaggcc tggccgatca caatcggccc ggaaccaatg atcagaattt tttggatatc
     3361 agtacgtttg ggcatggcgg tgtttttatc actaagagct tggggctgag ggcttggggc
     3421 ttggggaaat acaaattttt caggtattta gcctttagcc agcccgagat gacgaatctg
     3481 aaatggtacg aaaaatttca gaatgagaag acccaccggc tgatgcaagt gggactgact
     3541 ggtttcgggt ttctaacgca aaaggaatgg tggatgcaat ccagcccgca ggttgccaaa
     3601 cagtaccttc agcctttaga gtaacaactc acaggccatt cagcaccatt tcccataact
     3661 cattttcaac cactggtttt atcctcctgc ttatgttcac cttgaagtcc aatcattcga
     3721 ggtacttcgc gcttgcctgg cttgtggcaa gttgtgccat gacggcctgt gtcattccac
     3781 caggtggcta tttcgggacg attgtcgccc caaaagagca aaaaattgtc gttgggaatc
     3841 actctgaacc acgctcactt gacgcccaca agaccactgg cgtggcggaa agcaatatca
     3901 ttaaggaatt gtttgaaggg ctgacggtgt atcatccgga gacactggaa ccactgccag
     3961 ggagcgccgt ttcgtgggaa tcacaacagg acgcccaggt gtggatc
//
LOCUS       DAOQDO010000263         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15810_length_3999_cov_2.437120, whole genome shotgun sequence.
ACCESSION   DAOQDO010000263 DAOQDO010000000
VERSION     DAOQDO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15810_length_3999_cov_2.437120"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..790
                     /gene="dapB"
                     /locus_tag="PKZ53_05525"
     CDS             89..790
                     /gene="dapB"
                     /locus_tag="PKZ53_05525"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNJ39928.1"
                     /translation="MNIVLFGDGKMGHEIRKLAAERGFHVVGSVTRHEISGEPGNAKV
                     AIDFSHADAIGSHLNLAIELGLNLVIGTTGWNAQLPEVRRLVNSHGLGLMYSPNFSLG
                     VQLSFRIAQFAAQLVSAFETHQPYIVEAHHQFKKDAPSGTALRFQQLVSEPFKQEVPT
                     TSLRAGFIPGTHRVGFDSEFDTIELVHTARQRTGFADGALLAARWIAGKQGVFEFSTL
                     IDEQLNQTFGVPAKG"
     gene            874..1782
                     /gene="dapA"
                     /locus_tag="PKZ53_05530"
     CDS             874..1782
                     /gene="dapA"
                     /locus_tag="PKZ53_05530"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNJ39929.1"
                     /translation="MSNDVKLQLSGVGTALVTPFTKDFQLDEPALRRLVEFQINNQVD
                     FLVPGGTTGESATLSDEEISRLVDIVVQTTAGRVPVVAGAGGNNTAKVIKLAKQFEKL
                     GAQALLSVTPYYNKPTQEGLFQHYRAVAEATNLPILVYNVPPRTNVNLFPDTVARLAE
                     IPNIVGIKEASGDISQISEILARVPSNFLVFSGDDSLTLPVIALGGHGVISVISNELP
                     LLTSQLVHASLEGRFEDARTLHRHLLPLMKANFLETNPGPVKAVLAHFGLIQEVLRLP
                     LVPVHPSTRERLRTLLRELGLVEHRA"
     gene            1965..2795
                     /locus_tag="PKZ53_05535"
     CDS             1965..2795
                     /locus_tag="PKZ53_05535"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNJ39930.1"
                     /translation="MTLEDRINKLFEQPPETYSEAHRRLFHNFKAELDAGRIRAAEPN
                     DGQWKVNLWVKRGILLGFRLGQLQDFSVNSQIRFFDKHTFPPKPLKLTDDVRLVPGGS
                     VIREGAYVGRGVICMPPMYINVGAYVDEGTLVDSHALVGSCAQIGKRVHLSAGAQIGG
                     VLEPVGELPVIVEDDVLVGGNTGIYEGTIVRRRAIVGAGVVLTGSTPVFDLVHQRILQ
                     KQPNQPLEIPEGAVVIPGARAITKGWGAEQGLSIQTPLIVKYRDEKTDAAVTFEELLR
                     "
     gene            2860..>3999
                     /locus_tag="PKZ53_05540"
     CDS             2860..>3999
                     /locus_tag="PKZ53_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ39931.1"
                     /translation="MPIFSPISPSPARRVNAIQLSVLRQIYDQSPAGSINLGLGEPDV
                     LLSGVIREAAEAALAGDRLGYTPNAGLDEIREAIAAYQASITHVGYGRDNVCVTSGAQ
                     EALYTALMALVNPGDEVLIPDPGFLAYPTLVRLAGGYVKTYPLPAVSGFAFDHEAFAA
                     CLSPRTRLVIFNSPSNPTGTIFSSEDWEFVADQLSRHRILAITDEVYREIYADQAPPS
                     LAEVYDGTLVVSSLSKMLGMTGWRLGWAAGPTEVVRAVTVMHQYVTTCASTLSQRAAL
                     AAFTPAGLAAVQEIRETLQQRRQILQSGLAQMDVPFAIGPGAFYGLVDVSALGDDFQV
                     AMELLKECVITVPGSAFGIQSTGFLRLSFGGQPQNLREGLSRLERG"
BASE COUNT          928 a         1073 c         1056 g          942 t
ORIGIN      
        1 cattttgcaa tgacgtcttc acacgaaaaa acaatcaagt cattcaatca agcggagtta
       61 gctgactact gactactgac tactgactat gaatatagtt ttatttggcg atggaaaaat
      121 gggtcacgag attcggaaac tggctgccga acgggggttt cacgttgtcg gaagtgtcac
      181 ccggcacgaa atttccggtg agcccggaaa tgccaaagtt gctattgatt tttcgcatgc
      241 tgacgcgatt ggctcacatc tcaatctcgc gattgaactt ggtctcaatc tggtgattgg
      301 caccactggc tggaacgctc aacttccaga agttcgtcgg ctggtcaatt cacacggact
      361 cggcttgatg tacagcccga atttcagcct tggtgtgcaa ctgtcgtttc gaatcgctca
      421 atttgcggct caactggtca gtgccttcga gacacaccag ccctacattg tcgaagccca
      481 ccaccagttt aaaaaagatg cgccctctgg aacagccctc cgctttcagc aactcgtcag
      541 tgaaccgttc aagcaagaag ttccaaccac cagcctccgc gccgggttta tcccaggaac
      601 gcaccgggtt gggtttgatt cagaatttga cacgattgaa ctggtccaca ctgcacgcca
      661 gcgaaccgga tttgccgacg gagcactact ggccgcacgc tggatcgctg gaaaacaagg
      721 cgtcttcgaa ttttcgacgt tgattgatga acaattgaat caaacatttg gtgtgccagc
      781 caaaggctaa aacggcacat cgtgaagggt aattgattga acacactgtg ttcacctaac
      841 cctgcaaccc cgcaaccctg acgtggtaca agtatgagca atgatgtaaa gcttcaatta
      901 agcggcgtcg gaaccgccct ggtcacaccc tttaccaaag acttccaact ggacgaacca
      961 gccttgcgcc gacttgtcga gttccaaata aacaatcagg ttgacttttt agtgccaggc
     1021 gggacaactg gcgagagcgc gactttaagc gacgaagaaa tctcccgcct ggtggatatc
     1081 gttgtccaaa ccacggctgg ccgggtgccg gtcgtggcag gtgcgggcgg aaacaacacc
     1141 gccaaagtca tcaagctggc caaacaattt gaaaaactgg gggcgcaggc gctgttgtcg
     1201 gtcacgccct actacaacaa accgactcag gaaggtctgt tccagcatta tcgggcggtt
     1261 gccgaagcca cgaatctgcc gattcttgtc tataacgtac cgccccgaac caacgtcaac
     1321 ctgttcccag atacggttgc ccggctggct gaaatcccaa acattgtcgg aatcaaagaa
     1381 gcctcgggtg atatttccca aatttctgaa atcctggccc gcgtgccgtc aaacttcctg
     1441 gtgttttcgg gggatgattc gctgacgttg ccagtgattg cacttggcgg acatggcgta
     1501 atttcagtga tttccaatga actgccgttg ctgacctcgc aactcgtcca tgccagcctc
     1561 gaaggtcgat ttgaggatgc acgaacgctc caccgtcatt tattgccgtt gatgaaagca
     1621 aattttcttg aaaccaaccc cggaccggtc aaagccgtgc tggctcactt tggactcatc
     1681 caggaagtgc tgcgcctgcc actggtgccg gttcatccgt caacccgcga acgcctccga
     1741 acgcttttgc gtgaactcgg cctggttgaa caccgggcct gagcaaacga tgtcgcgtgc
     1801 agcctgaagc agcgaaagaa tcagtggaaa aacccctgac tcagaaagcc tgagcaaaac
     1861 cggccaggac caattaagcg actatttttc aacaatttaa cccgttgtga acgcgttcac
     1921 aatttgtctt tgacacgctt cgcgacttca ctctttcgtt ccccatgaca cttgaagacc
     1981 gtatcaacaa actcttcgaa cagcctccgg aaacctattc ggaggcgcat cgtcggctgt
     2041 ttcataattt caaggccgag cttgatgctg gacggatccg tgccgcggaa cccaacgatg
     2101 gccagtggaa ggtcaacttg tgggtcaaac gcgggatttt gcttggattt cggctcggac
     2161 aacttcaaga cttttcggtc aattcgcaga ttcgcttttt tgacaaacac acgtttcctc
     2221 caaaaccatt gaaactcacg gacgacgtgc ggctggttcc gggcgggtcg gtgattcgag
     2281 aaggtgccta tgtcggacgc ggtgtgattt gtatgccgcc aatgtacatc aatgtcggcg
     2341 cctatgttga tgaaggaacg ctagtggatt ctcacgcact ggttggttcc tgcgcccaga
     2401 ttggaaaacg ggtgcatctc tcggctggtg cccaaatcgg cggtgtcctc gaacccgtcg
     2461 gagaactccc ggtcattgtc gaagacgacg tgctggtggg cggtaacacc ggcatttatg
     2521 aaggaacaat tgttcgacgc cgggccattg ttggtgccgg ggttgtgctg acgggttcaa
     2581 ccccggtgtt tgatctggtg caccagcgaa ttcttcaaaa acagcccaat caaccgctgg
     2641 aaattccgga aggcgcggtg gtcattcccg gagcgcgagc catcacgaaa ggctggggtg
     2701 ccgagcaggg gctttcgatc cagacgccgc tgattgtgaa ataccgcgat gaaaaaaccg
     2761 atgcggcagt gacctttgag gagttgttga ggtgatttgc gggttccggg ttcttcgatt
     2821 tatttccttt tttgttcttt ctgcgctttt cccaaaacca tgcctatatt ttcaccaatc
     2881 tcgccatcac cagcccgacg cgtcaatgcc attcaactca gcgtgttgcg acaaatttat
     2941 gatcagtcac ctgctggttc aatcaacctt gggctgggtg aaccagatgt tctgctatct
     3001 ggagtcattc gcgaagccgc cgaggccgca ctggctggcg accggctggg atacacacca
     3061 aatgccgggc tggatgaaat tcgagaagca atcgctgcct atcaggcttc aatcacacat
     3121 gtgggttatg gtcgggacaa tgtttgcgtg accagcggcg cccaggaagc gctctatacg
     3181 gcactgatgg cactggtcaa tccgggagac gaagtgctga ttccagaccc tgggtttctg
     3241 gcatacccga cactggttcg attggcggga ggatacgtca agacgtaccc gcttccagcc
     3301 gtttccgggt ttgcctttga ccacgaagcg tttgccgcct gtctttcgcc acgaaccaga
     3361 ctggtgattt ttaattcgcc ttcgaatcca accggcacca ttttttcaag cgaagactgg
     3421 gagtttgtcg cagaccagct ttctcgccac cgaattctgg ccattaccga cgaagtctac
     3481 cgcgaaatct atgcagacca ggcgcctccg tcactggctg aggtctatga cggaacactg
     3541 gtcgtgtcga gtctttccaa aatgcttgga atgactggct ggcgacttgg ctgggcggca
     3601 gggcccacag aagtggtacg cgccgtgacc gtgatgcacc agtacgtaac gacctgtgcg
     3661 tcaaccctgt cgcaacgagc ggcgcttgcg gcatttactc cggcaggact ggcagcagtt
     3721 caggaaatca gagaaaccct tcagcagcgg cgccagattt tgcagtcagg actggcccaa
     3781 atggatgtgc cattcgcgat tggaccaggc gccttttacg gactggttga cgtgtcagca
     3841 cttggcgatg attttcaggt ggcgatggaa ttgctcaaag aatgtgtgat taccgttcca
     3901 ggaagcgcct ttggaataca atccaccgga tttcttagat tgtcgtttgg cggtcagccc
     3961 caaaatctgc gtgaaggctt gagccgactg gaacgaggt
//
LOCUS       DAOQDO010000264         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15822_length_3997_cov_3.171740, whole genome shotgun sequence.
ACCESSION   DAOQDO010000264 DAOQDO010000000
VERSION     DAOQDO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15822_length_3997_cov_3.171740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..625
                     /locus_tag="PKZ53_05545"
     CDS             149..625
                     /locus_tag="PKZ53_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNJ39932.1"
                     /translation="MTLKLDLAKMKLISGFVDTYLNLKPDERVQFEREVEAFQPNEKE
                     KVMELTTSWKEEGIQQGLQQGLQQGLQQGLQQGLGQGMEREGKLFALKLLKRRFGDLP
                     AWMVESIERLKLQQIEQLGDVLLDFQTLADLETWLKNISESLVLPDSSSVEGLEKG"
     gene            1004..>3997
                     /locus_tag="PKZ53_05550"
     CDS             1004..>3997
                     /locus_tag="PKZ53_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39933.1"
                     /translation="MRCPHCQADFPSGLTVCPKDGHMLMETSNESFTGFVLDGKYELL
                     HQLGSGGMGVVYLAQQKSTGKQVAVKIRHQESKAELDRFQREAQAASLMNHPHSVTIL
                     DYGITADGIYYIAMELLQGPSLSEELAAKGFFSFDRTLELVRPVAQALSAAHDLGLIH
                     RDLKPSNVLLHRPSPGVEIVKVVDFGMAKMDQLDQSTAQLTGTGWVLGTPEYMSPEQC
                     LGHTVDPRTDIYSFGVMLFELLTGHLPFRDTTISNILLKQISEHPPYLRRLRPELPAA
                     LESFILRLLSKLPSSRPGTLSSVLAELEGIWQRWHQEAGSHPHRDTSLETGPPLPLPD
                     PLPASTSYISSANAPTLVATLLDVPTAWELDERETLSGCYLRTAFIRRFEDWLLKTSP
                     IPPETPLGCLLVLELVGWEAWRTTAQIEDVEQALRDTRQILARVFGGAALLGRYSESR
                     WLVALPWPESPNLNAKSRIENFAHKSVLEFHRTVQRNPLLGTAARLVVSAGGVLFRRG
                     EQAQPLITIALERLQRARKKERGTVILENEPTVVPAGKALEATFLKALRTGPLPQLEL
                     SAETEFVGLLKEHRFVGRDTVIDRLRNAFYQNLRDVAYPAWILGDPGTGKSYLLEQLA
                     DQLALKDVFIRQVQFTQAERDEGLSIFYEALRDLLKRLVEEDPESLPATFGTLTDRVR
                     EDFLEREAISQVILNPSGRDLPSLAETQKIEQFEYFTRVFGLIARCRPVILCVDDVHC
                     AKPTGLEFLSYLLRRSSLERFFLILTLCRPEVERDVSPVKSWFRQVQRSFPGELINLP
                     EFTLHDVRRLVESMVWPSQVCEETICRLMLETKGNPRYLKELVILLHKENRLVYGSQR
                     WRLTAETGSLPVPACVLREAEATVAQLDPEAQSVLSRAAFIHERFAFDVVRDLTHLPE
                     GTLLEILEDALERQILLEDEDVQDGYRFANPVVWRILAERLDQEEGADLHRQVCTLIE
                     PYASGWAAWAFPLSEHYLKAGHWDAA"
BASE COUNT          910 a          983 c         1101 g         1003 t
ORIGIN      
        1 atttcaatat cgggtgattc agttgaaccg attgaactgg cgtgatttcg tcaatcgtcc
       61 aaacccagtg gcggcagcgt tgatggcaaa aatgaggatt gccaaagcgg accggccacg
      121 ggtgaaagca caatgtctcc gattattggt gacgctcaaa ctagatttag cgaaaatgaa
      181 gctgatttca gggtttgttg acacctacct gaacctgaag ccggatgaac gagttcaatt
      241 tgaacgagaa gtagaggctt ttcaacccaa tgaaaaggag aaagttatgg agttgacaac
      301 aagttggaaa gaagaaggaa ttcagcaagg attgcaacaa ggattgcaac aagggttgca
      361 acaagggttg caacaaggac tcgggcaagg aatggaacgt gagggaaaac tttttgccct
      421 aaaactcttg aaacggcgat ttggagatct cccagcgtgg atggtcgaaa gcattgaaag
      481 actgaaactt caacaaattg aacaacttgg agatgtattg ctggactttc aaacactggc
      541 tgatcttgaa acctggctca agaacatcag tgaatctttg gttttgccag atagttcttc
      601 agttgaagga ctcgagaaag gctgagttct gatggagctt cagtgggaaa gtcgccttga
      661 gttttgcttc agccagtcct gccgtagtcg atagagaaaa tagcttctgg tgcaaccggg
      721 gaatcaaaca ccgtcttctt ctgggccaat cagcaggtgc ttgagccgaa agtctttgcc
      781 ttttcaccct ccatcttttt atactccacc gccgggtaat tcacagcccg tttggattct
      841 tcttcaattc actaccagac ctggcaggtc ggtgaatcag cgtgtcgtct ccccaatcta
      901 ttgattttca ttggtttatt tttttggaat gtgatgacgg tcgagcgggc ctgtccctgg
      961 gaatcagggt ggcataagtg tctttctctg cgagaaatca gccatgcgat gtccacactg
     1021 ccaagctgac tttccatcgg ggctcacggt ctgccccaag gatgggcaca tgctgatgga
     1081 aaccagcaac gaatctttta ccgggtttgt gctcgacggc aaatatgaac tgctccacca
     1141 gttgggcagt ggtggcatgg gggttgtgta cctggcacaa caaaaatcca ctgggaaaca
     1201 ggttgcggtc aaaatccgtc atcaggaatc aaaagctgag cttgatcggt ttcaacgtga
     1261 agctcaggct gcctcgctga tgaaccatcc ccactcggtg actattctag attatggcat
     1321 cacggcggat gggatttact atatcgcgat ggaattgctt caggggccaa gcctgagtga
     1381 ggaactggcc gccaaaggtt ttttttcgtt tgaccggacg cttgaactgg tgcgacctgt
     1441 ggcgcaggcc ctgagcgccg cccatgatct gggcttgatt caccgtgatt taaagccgtc
     1501 aaatgtcttg cttcatcggc catcaccggg agttgaaatc gtcaaagtgg ttgattttgg
     1561 catggccaaa atggatcagc ttgatcagtc aacggcgcaa ctcactggaa ccgggtgggt
     1621 tttgggaacg cctgagtata tgtccccgga acaatgtctg ggccacacgg ttgatccccg
     1681 aactgatatc tacagttttg gcgtgatgtt gtttgaactc ctgaccggac acctgccgtt
     1741 tcgcgacacc accatttcaa atattctgct caaacaaatt tcagagcatc ccccgtattt
     1801 gcggaggctt cggccagaac ttccagcggc gctcgaatct tttatcctgc gtttgctcag
     1861 taaattacct tccagccgac ctgggacact gtctagcgtg cttgccgaac tggaaggcat
     1921 ttggcagcga tggcaccagg aagccgggtc tcatcctcat cgagacacca gcctggagac
     1981 cggcccgccc ctccccttac ctgacccgct ccctgcatcc acttcatata tttcatcagc
     2041 caatgcccca accctggtcg caaccttgct tgatgttcca accgcctggg agctggatga
     2101 gcgtgagaca ctcagcggat gttatcttcg aacggccttt attcgacggt ttgaagactg
     2161 gctgctcaag acctcaccta tcccgcccga gaccccgttg gggtgtttgc tggtactgga
     2221 actggttgga tgggaagcct ggcggacaac cgcccagatt gaagatgttg aacaggcgtt
     2281 gcgcgatacc aggcagattc tggcccgggt ttttggtggt gcggcgcttt tggggcgcta
     2341 tagtgaatcc cgctggctgg tggccttgcc ctggcctgaa tccccaaatc tcaatgcaaa
     2401 atcgcgaatt gaaaactttg ctcataaatc ggtgcttgag tttcatcgaa ccgtacagcg
     2461 caatcccttg cttggaaccg cagcccggct ggtggtttcg gctggtggtg tgttattccg
     2521 gcgcggcgag caggcccagc ccctgattac gattgccctg gagcgactcc aacgggctcg
     2581 aaagaaagag cggggaacgg tcattttgga aaacgagccg accgtggtgc cggctgggaa
     2641 agcacttgaa gcgacatttc tcaaagccct gcgcaccgga ccgctccccc aactggaact
     2701 ttcggctgaa actgaatttg tcgggttgtt aaaagagcat cgttttgttg gtcgcgatac
     2761 ggtgattgac cgcctgcgga acgcttttta ccagaacctc cgggacgtgg cctatcccgc
     2821 ctggatcctt ggtgatccag gcacagggaa gtcttattta ttggaacaac tggctgatca
     2881 gcttgctctc aaggatgtgt ttatccggca ggtgcagttt acccaggctg agcgtgatga
     2941 agggctctcg attttttacg aggctctgcg tgatttgctc aaacgactgg tcgaagaaga
     3001 ccccgaaagc ctgccagcta cttttggaac cttaactgac cgggttcggg aagattttct
     3061 tgagcgagaa gccatctccc aggtgattct caatccatcc gggcgtgatc ttccttcgtt
     3121 ggctgaaaca cagaaaattg agcagtttga gtactttacc agggtgtttg ggttgattgc
     3181 gcggtgtcgt ccggtgattt tgtgtgtgga tgacgtgcat tgcgccaagc cgaccgggct
     3241 ggaattcctg tcatatttat tgcgtcgaag cagcctggaa cggttctttc tgattttgac
     3301 gctctgccgc cctgaggttg agcgggacgt cagcccggtg aagtcgtggt ttcgccaggt
     3361 tcaacgatcc tttccagggg agttaatcaa tttacctgag ttcacgcttc atgacgtgcg
     3421 ccgactggtt gagtcaatgg tgtggccatc gcaggtctgt gaagaaacca tttgccgttt
     3481 aatgcttgaa accaaaggca atcccaggta tttgaaagag ttagtgatat tgctccacaa
     3541 agagaaccgg cttgtttatg gatctcaacg ctggaggtta actgccgaga ccggttcttt
     3601 gccagttcca gcctgtgtgc tccgtgaggc agaggcaacc gtggcccaac tggatccaga
     3661 ggctcagtcg gtgctgtcga gggcggcgtt tatccacgaa cgatttgcat ttgatgtggt
     3721 ccgtgacctg acacatcttc ctgaaggcac actgctggag atcctggaag atgcgctgga
     3781 gcgacaaatt ttgctcgaag acgaagacgt tcaggacgga tatcggtttg ccaacccggt
     3841 cgtatggcgg attctggctg agcgccttga tcaagaggaa ggtgcagacc ttcaccggca
     3901 ggtctgtaca ctgattgaac cttatgcctc ggggtgggct gcctgggcat tcccgctgtc
     3961 tgagcattac ctgaaagccg ggcactggga tgcggcg
//
LOCUS       DAOQDO010000265         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15850_length_3994_cov_2.832445, whole genome shotgun sequence.
ACCESSION   DAOQDO010000265 DAOQDO010000000
VERSION     DAOQDO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15850_length_3994_cov_2.832445"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..3740
                     /locus_tag="PKZ53_05555"
     CDS             108..3740
                     /locus_tag="PKZ53_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNJ39934.1"
                     /translation="MDQLPDFAFDPTRMVDELAGSQTPLLIGVRHHSPACAAAIPRLL
                     DAFGPDKVVIELPTEFQDWIKWLGHKDLLAPVALAGVRSDGNGLAFYPFADFSPELAA
                     IRWAIRQNIPVEAFDLPMEQWERLERVLREADPQTEVDHTEPVPSLLQQLLAQTNVED
                     SESLWDVLVESHATASDPEAIRRAALLYGWALRVESIRSKTVRNHDLHRERFMRECLF
                     KSGLSCFGSSAGAGEAVQPMPRIAAVIGAFHAPALLQHPWYWSPTEPAAKPAEKKKPS
                     RATTINTSLIAYSHELLDSRSGYPAGIRDPLWQHRVWEAASTGRTLSTVITESVVGIC
                     AEVRRKFHVASVPDAMEAVRMTEDLATLRGIPSPGRRELLEAIETALGQGELLGRGRI
                     LAQAMERVLVGRRRGRLPGGTPRSGLGPHVEQLLAELKLPGPGTESSEAKRMRLDPLR
                     SDLDARRQIAFKRLGACGVPYAKLEAGEAIGASELLTQVWTVSWTPGTEAMLELVGVR
                     GVTLEQATLGSLKAEEARYRSDDKWVPAVHLYLLGQAADCALHELTHDYLTALTQTFA
                     TEATLPQLIAAIELVLRIEHEHIPGLSFQNQKLIVDFDYIRAELFSAALKAIEGLAGS
                     KTLDDARALLELIQLFERQSEDPNSVGDGRLLWALNELAENGSSLMQGAAGALRVRLG
                     VTAGAEFGRLVASWVDAATSKDTHQTLAERLQGTLAVAAPLFEADPTWATPLMERIEQ
                     QTDTEFLHRLPALREGFEVLSTASRARMLTKLAERLEDDGITQTFDIVLNDNPHLLAR
                     CAEIDRTGRQAVESLFGQLPTVSAGNQRAADQALPKSPASGPDCAISPRDRWRLILGH
                     ERERMKPKSAGIARALDELYGAGHGEGSRGEIAGGKGGGKEAPFPSTREWAEELEALF
                     GGTIREEVLGRAVERGRTSALLELDAETVSPSIELLEQVLSLKGGLSEGQLGHLRRIV
                     QRVIDALVKELATRIRPALTGLTVPLPTRKPGGPLDIRRTITANLKTLRQDEAGNVTF
                     LPERLLFKTRTKRSMDWRIVLVVDISGSMEASVIYSAMMAAILSGLPAVSVHFVAFNT
                     EILDLTHRVEDPLGLLLEVSVGGGTHIARGLRYARELLTVPSRSLVILVTDFEEGWPV
                     AGLVEEVRALVETGAKTIGLAALDDSGQPRYSKSIAELVVGAGMPVAALTPLELARWV
                     GEKIRG"
BASE COUNT          874 a         1079 c         1146 g          895 t
ORIGIN      
        1 tggttcttgg ttcttcttcg aaaatattga tttccaagca ccaagcacca agcaccaagt
       61 actaagcacc agcacattct tcattcttca ttctccattc ttcatttatg gaccagcttc
      121 ctgattttgc atttgatcca acacggatgg ttgatgaact ggccggcagt caaacaccgc
      181 tcctgatcgg agtgcgtcat cattccccgg cctgtgcggc ggcaatcccc aggctgcttg
      241 acgcctttgg gccagacaaa gtcgtgattg agcttccgac cgaatttcag gactggatca
      301 agtggttggg tcataaggac ttgctggccc cggtggctct ggcgggcgtc aggtcagatg
      361 gcaatggtct ggcgttttat ccgtttgctg atttttcccc ggaactggcc gccattcggt
      421 gggccatccg gcagaacatc ccggttgaag ccttcgacct gcccatggag caatgggaac
      481 gcctcgaacg tgttctccgc gaggccgacc ctcagaccga agttgatcac accgaaccgg
      541 ttccttcact gctgcagcaa ttgctggctc aaaccaatgt cgaagattca gaaagtttgt
      601 gggatgtcct ggttgaatcc cacgccacag cctctgatcc agaagcaatc cgccgggccg
      661 cgcttttgta cggttgggcc ttgcgagtgg aaagtatccg gtcaaaaaca gttcgcaacc
      721 acgatctgca ccgcgaacga tttatgcggg aatgtctgtt caagtcaggg ttgagttgct
      781 ttgggtcgtc ggctggggct ggtgaagcag ttcaacctat gccccggatt gcggcggtaa
      841 ttggggcctt tcacgctccg gcactgctcc aacacccgtg gtactggtcg ccgacagaac
      901 ctgccgccaa accagcggaa aaaaagaaac ccagccgggc aaccacaatc aacacgtcgc
      961 tcattgccta ttcgcacgag ttgctggatt cacggtcagg atacccggca ggcatccgtg
     1021 acccgctctg gcaacaccgg gtctgggaag ccgccagcac cggacggaca ctttcaactg
     1081 tcatcacaga atcagtcgtc ggaatctgtg ccgaagtccg ccgcaagttt catgtcgcga
     1141 gcgtaccaga tgccatggaa gctgttcgca tgaccgaaga tctggcgacc ttgcggggaa
     1201 ttccgtcgcc aggccgtcgt gaattactcg aagcgattga aaccgcgctt ggccagggag
     1261 aattgctcgg acgagggcga attctggctc aggctatgga acgtgtcctg gttggtcggc
     1321 gccgaggaag acttcccggc gggacgcctc gttcgggtct gggtccacac gttgagcaac
     1381 tgctggctga gttaaaactt ccggggccag gcactgaatc aagcgaagcc aaacgcatgc
     1441 ggcttgatcc actccgttca gatttggacg cccgtcggca gattgccttc aaacggcttg
     1501 gggcctgcgg cgtgccctat gccaaactcg aagctggcga agccattggc gcgtcagagc
     1561 ttttgaccca ggtgtggacc gtttcgtgga cgcccggcac cgaagccatg ctggaactgg
     1621 ttggtgtgcg cggtgtcaca ctggaacagg caacgctggg cagcctcaaa gccgaagaag
     1681 cccgctatcg aagcgacgac aagtgggtcc cagccgtgca tttgtatttg cttggtcaag
     1741 ctgctgattg tgccctccac gaattgaccc acgactattt aaccgctttg actcagacgt
     1801 ttgccaccga ggcaactttg ccacaactga ttgctgcaat tgaacttgtc ctgcgcatcg
     1861 agcacgagca cattcctggc ctttcctttc aaaaccagaa actaatagtt gattttgatt
     1921 acatccgggc ggaattgttt tcagcagcgc tcaaagcaat tgaaggactg gccggatcaa
     1981 aaacgcttga cgatgcccgg gccttgctgg aattgatcca gctttttgaa cgtcaatctg
     2041 aagacccaaa cagtgtcggt gatggtcggc tgctctgggc actcaacgaa ttggcggaaa
     2101 atggctcgtc actgatgcag ggggctgccg gtgcgttacg ggtcagatta ggggttacgg
     2161 ctggagctga atttggcagg ctggtggctt cgtgggtaga cgcggccacc agcaaagaca
     2221 cccaccaaac gctggctgaa cggttgcagg gcacgctggc ggtggcggcg ccactgtttg
     2281 aggctgaccc aacctgggcc acaccattga tggaacggat tgagcagcaa acagatactg
     2341 aatttctgca ccgattgccc gcacttcgtg aagggtttga agtcctttca accgccagcc
     2401 gggcccggat gctgaccaaa ctggccgaac gtttggaaga tgacggtatc acccaaacct
     2461 ttgacatcgt gctcaatgac aacccgcacc tgctcgcccg ctgtgctgaa attgaccgaa
     2521 cgggacgaca ggcggttgag tcactctttg gccagcttcc gaccgtttct gctggaaatc
     2581 aaagggcggc tgatcaagca cttcccaaat caccagcttc aggcccggac tgtgcaattt
     2641 cgcctcgtga ccgatggcgg ttgattctgg ggcacgaacg cgaacgaatg aagcccaaat
     2701 cggctggaat tgcccgcgcc ctggatgaac tctatggtgc cggtcacggt gaaggctcgc
     2761 gcggggaaat tgccggaggg aagggaggcg gaaaagaagc accgttccct tcaacccgtg
     2821 aatgggccga agaactggaa gccctgtttg gcggaactat ccgcgaagag gtgctgggtc
     2881 gcgccgtcga acgtggccga acctcagccc tgctcgaact ggatgccgaa acggtttcac
     2941 cttcgattga attgcttgaa caggttcttt ccctcaaagg cggattgtcg gaaggccagc
     3001 ttgggcacct gcggcgcatt gttcagcgag tgattgatgc cctggtcaaa gaactggcca
     3061 cacgcatccg cccggcgttg acaggactga cggtgccgct tccgacgcgt aaacctggcg
     3121 ggccgctcga tattcggcga acaatcaccg ccaatttgaa aacacttcgg caggatgaag
     3181 ccgggaacgt cacgtttctt ccagaacggc tcttgtttaa gacccgcacc aaacgctcaa
     3241 tggactggcg gattgtgctg gtggttgata tttcgggttc gatggaagct tcggtgattt
     3301 atagcgcgat gatggccgcg attttgtcag ggttaccagc cgtttcagtt cattttgtgg
     3361 cgttcaacac tgaaattctg gacctgaccc atcgggttga agatccactt gggttgctgc
     3421 tcgaagtttc agtcggtggc ggaacacaca ttgcccgagg gctgcgctat gcgcgtgaat
     3481 tgttaaccgt tccatcacgt tcgttggtga tactggtcac tgattttgaa gaaggctggc
     3541 ccgtggctgg tctggtcgag gaagttcggg cactggtgga aactggcgcg aaaaccattg
     3601 gcctggcagc actggatgat tccgggcagc ctcgctatag caaatcaatt gctgaacttg
     3661 tggttggtgc cggaatgcca gtcgcggctc tgacaccgct ggaacttgcc cgctgggttg
     3721 gagaaaagat tcgcgggtaa cgcagatcga acaaagcagc ctggagcaga ttggggctga
     3781 gggtatgggg ctaggggctg aggaaaatac aaggctttca ttcgcaaacg gtttaatccc
     3841 aatttgcaat cgagtcctcc tattagaaag tagttcaata gtgcaatcaa ataagcctgg
     3901 cgaactactg actactgact actgactggt atcagttcaa ttctgtatcc ggttcagttt
     3961 tccaccagct tccaatcgga aacggtctcc gcgc
//
LOCUS       DAOQDO010000266         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15860_length_3991_cov_3.557927, whole genome shotgun sequence.
ACCESSION   DAOQDO010000266 DAOQDO010000000
VERSION     DAOQDO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15860_length_3991_cov_3.557927"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKZ53_05560"
     CDS             complement(<1..805)
                     /locus_tag="PKZ53_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ39935.1"
                     /translation="MPSKLTATKSIDELMKESNNPEHGLKRALGRLNLTTLGVGAIIG
                     AGIFVLTGSAAAQYAGPAIVLSFIVAGIGCLFAGLCYAEFASMIPIAGSAYTYAYATM
                     GEFLAWIIGWDLILEYLFGASTVAVGWSGYIVSFLKDLGLVIPPALSQAPLAYNAATH
                     GWTQTGAMMNLPAMFIVALMTALLVVGIKESAYFNNLIVIIKIVVILLFVGFGFAYIK
                     PANLTPLIPENTGTFGVYGWSGILRGAAVIFFAYIGFDAVSTAAQEAKDP"
     gene            complement(1207..3282)
                     /gene="tkt"
                     /locus_tag="PKZ53_05565"
     CDS             complement(1207..3282)
                     /gene="tkt"
                     /locus_tag="PKZ53_05565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNJ39936.1"
                     /translation="MSTPRPSGSVDTLCVNAIRTLSMDAVQAANSGHPGTPMAMAPVA
                     YTLWNEFLHFDPEDPIWPNRDRFVLSMGHASMLLYSMLHLTGVKAVNSVYEKTGEPSV
                     PLDDIKRFRQLDSRCPGHPEYRWTSGIETTTGPLGQGIATSVGMAIAGKWLGATYNRP
                     GFDIFNYRVYALCGDGCLMEGISQEAASLAGHLKLNNLCWIYDNNKITIEGNTSWAFS
                     EDVATRFIGCGWNVTRVGDANDLDMLRRAFLTAQKESDRPTLIIVDSHIAYGAPNKQD
                     THAAHGEPLGEEEIRLTKRNYGWPEDAKFHVPEEVYEHFRKGIGARGHQGRNEWIVKF
                     NEYNKAYPDLAEQVFRMQKRELPEGWDKDLPTFPADPKGLAGRDASGKVLNALAKNVP
                     WLIGGSADLAPSTKTRLTFPEAGDFTADNYAGRNFHFGIREHAMAAALNGLSLSKIRP
                     YGSGFMIFSDYARPSIRLSSIMEIPTIHIFTHDSIGVGEDGPTHQPVEHLASLRAIPG
                     LLVIRPADANEVTEAWRNIMQIKHEPVALILTRQALPTVDRTRFASAENVKYGAYVLA
                     DPPDGAAPDVILLASGSEVSLCVEAYEQLTREGINARVVSMPCWEIFEHQSAEYRESV
                     IPDSIQARVAVEQASTFGWGRYVHHRDAIIGMHTFGASAPLKELTKKFGFTLENVVAT
                     AKREIERAK"
     gene            complement(3742..>3991)
                     /locus_tag="PKZ53_05570"
     CDS             complement(3742..>3991)
                     /locus_tag="PKZ53_05570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNJ39937.1"
                     /translation="DMQAHQQVVFRYATPEGHITDEANPNGSLDNIAGIINRAGNVLG
                     MMPHPERACEESLGGSQGLRIFDSLLTSVIERLKARDA"
BASE COUNT          960 a          975 c         1150 g          906 t
ORIGIN      
        1 gagggtcttt ggcttcctgg gcggcggttg aaaccgcatc gaatccaatg taagcaaaga
       61 aaatgaccgc ggcgccgcgc aggataccgc tccagccata cacgccaaac gttccagtat
      121 tttctggaat caagggggtc aggttggctg gtttaatgta ggcaaatcca aatccgacaa
      181 agagcaagat cacaacaatc ttaatgatca cgatcagatt gttaaaatac gcggattctt
      241 taatcccaac gaccagcagt gcggtcatca gggccacaat aaacattgcc ggcagattca
      301 tcatggcacc ggtttgagtc cagccatggg tcgcggcgtt atacgccagc ggggcctggg
      361 agagggcggg tgggatgacc agacccaggt ctttgaggaa actgacgata tagcctgacc
      421 atccgacggc gacggtggaa gccccaaaca gatattcaag aatcaaatcc cagccgataa
      481 tccaggccag gaactctccc atggtggcgt aggcgtaggt gtaggcactt ccggcgatgg
      541 ggatcatgga ggcgaattcg gcatagcaca acccggcgaa caggcaccca attccagcca
      601 cgatgaatga aagcacaatc gccgggccgg catattgggc tgctgctgaa ccggtcagga
      661 caaaaatacc agccccgata atggcgccga ctccaagcgt tgtcagattc aaccgaccca
      721 gggcacgttt gagcccgtgt tcagggttgt tggattcctt catgagttca tcaattgatt
      781 ttgtagctgt gagcttactt ggcatgcgct ttggtcctca taaattatcg attgaatgcc
      841 atcgccgggt gacttaaaag caattctccg agcaggtagg gaaatggtca aacgactccc
      901 ccagccggtc aatcgctgga gcgtaactga cctggccgct ggttgctgat tctcacctgc
      961 ctgggtgagg gttgaagttt tggtgaaaat caaaagctgg gagaagataa gtcaagcgat
     1021 gacgaaatgt ggcgcacagt atagccaaac ttgggaaggg atcaacttta ttcttgagcg
     1081 aagggagggc aaggggtgag gagtggtgga gcaaatggcg aggagcaaag gagcaaggtg
     1141 tgtcatcgac gagaattttg ggggcttctc attcggtcct tcgctccttc gctcttgatt
     1201 gaaaagctat ttggcgcgtt cgatttcacg tttggccgta gccaccacat tttcaagggt
     1261 aaatccaaat ttcttggtca gttccttgag tggcgcactg gcaccgaagg tgtgcatgcc
     1321 gatgatggca tcacgatgat ggacatagcg cccccaccca aaagtggaag cctgctcgac
     1381 cgcaacgcgg gcctgaatcg aatctggaat cactgactcg cgatactcag ccgactgatg
     1441 ttcaaagatt tcccaacagg gcatggacac aacgcgggcg ttgatgcctt cgcgggtcag
     1501 ttgttcatag gcttcaacac acagtgaaac ttcggatcca gaagcgagca ggatgacatc
     1561 tggcgccgca ccatccggcg ggtcagccag gacataggcc ccatatttga cgttctcagc
     1621 actggcgaaa cgggtccggt caacggttgg gagggcctgg cgggtcaaaa tcaatgccac
     1681 aggctcatgt ttaatctgca tgatgtttcg ccaggcttca gtgacttcgt tggcatcggc
     1741 tggtctgatc accagcagtc caggaatggc ccgcagtgag gccaggtgct cgacgggctg
     1801 gtgcgttggt ccatcctcgc ccactccgat tgagtcatgg gtgaaaatat gaatggttgg
     1861 aatttccata atcgagctga gccggatgga tggccgggcg taatctgaaa aaatcatgaa
     1921 accagagccg tacgggcgaa tcttggacaa ggagagcccg ttgagagccg cggccatggc
     1981 atgttcccga ataccaaaat ggaaattccg cccggcataa ttgtcagccg taaagtcacc
     2041 ggcttcgggg aaggtcagtc gggttttggt cgagggggcc aggtcggctg agccaccaat
     2101 caaccacgga acatttttgg ccagtgcatt gagaactttt ccagaggcat cacgacccgc
     2161 caggcctttg gggtctgccg ggaaggtcgg gaggtctttg tcccagcctt cgggaagttc
     2221 acgtttttgc atgcgaaaaa cctgttcggc cagatcggga taggctttgt tgtattcgtt
     2281 aaatttgacg atccactcgt ttcgtccctg gtggccacgg gcgccgattc ctttacggaa
     2341 gtgttcatag acttcttcgg gaacgtggaa tttggcatct tcaggccagc catagttgcg
     2401 ctttgtcagt cggatttctt cttcgccgag cggttcgcca tgagcggcgt gggtgtcctg
     2461 cttattcgga gcgccatagg caatatggct atcaacgata atgagggtgg ggcggtcaga
     2521 ctctttttgg gcagtgagaa aggcgcgacg gagcatatcg aggtcattgg catccccgac
     2581 gcgggttaca ttccagccgc atccgataaa acgagttgca acgtcctcgc taaaggccca
     2641 ggatgtattt ccctcaatcg tgattttatt gttgtcataa atccagcaca gattgttcaa
     2701 ttttaaatgc ccggccagcg acgcggcttc ctgggaaatt ccttccatca ggcatccatc
     2761 accacacaac gcatacaccc gatagttaaa aatatcaaac cccggtcggt tatacgtggc
     2821 gcccagccat ttgccggcaa tcgccatccc gacgctggtg gcaatgcctt gacccagcgg
     2881 cccggtggtg gtttcgatcc cggaggtcca gcggtattcc gggtgaccag ggcagcgact
     2941 gtcaagctgg cgaaagcgtt tgatatcatc aagtggaacc gatggttcac cagttttttc
     3001 atacacggaa ttgacagctt tgacacctgt caaatggagc atgctgtaga gcagcattga
     3061 tgcgtgcccc atggacaaaa caaagcgatc ccgattgggc cagattggat cttcgggatc
     3121 aaaatggaga aattcattcc agagtgtata ggcaacaggc gccatggcca tgggggttcc
     3181 tgggtgacct gagttagcgg cttgcacggc atccatcgat aaggtgcgga tcgcattcac
     3241 gcacaatgta tcgactgaac cagatgggcg tggtgtactc ataacaattc gtcctctttg
     3301 gtttgttgag ctgaaggtgt gacttctgag ccgaaggcag acggtgtgtg ggctgcctgg
     3361 ccgggttgaa gaaccattcc tggtgcgtgg tcagggctta caatgggagg tcaaagccag
     3421 cgaccatacc gcgttcagga gcaagtggtt ttcaagagaa agaattcggt ttggttggtt
     3481 tgggtgaaga cttccacagc aatctggata agagaggaat ggtgctgtgg gggtgatcaa
     3541 tcagcggtat tttttcaccg agagcatacc gtagtggctt tctcttgtca tgaagaaaaa
     3601 tttcaaagcc tgcggcttta ttccagcaac caagagaaag atcaactggc agagggatag
     3661 cataacaggt gacagaagaa gcgggagacc agatgacagg caaagcagag cctgtcatct
     3721 gctctcccgg ccataccaga gtcaggcatc gcgggctttg aggcgttcga tgactgaggt
     3781 gaggagggag tcaaagattc tcaaaccctg acttccaccc agactttctt cacaggcccg
     3841 ctcggggtgg ggcatcattc cgagtacatt cccggcacga ttgataatcc cggcaatatt
     3901 gtcgagtgag ccgttggggt tggcttcatc tgtaatgtga ccttctgggg tggcatagcg
     3961 gaaaacaacc tgttgatgag cctgcatgtc t
//
LOCUS       DAOQDO010000267         3973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15973_length_3973_cov_2.507402, whole genome shotgun sequence.
ACCESSION   DAOQDO010000267 DAOQDO010000000
VERSION     DAOQDO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15973_length_3973_cov_2.507402"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKZ53_05575"
     CDS             complement(<1..331)
                     /locus_tag="PKZ53_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HNJ39938.1"
                     /translation="MSPDNRTHRGAHPEDHTLFAPDQVERLRLATAELSWLLSRGYQL
                     NSSLKLVGDRHSLRERQRLALSRTACPDHLLQLRDTKQLPLEAVHNQVIVIDGFNLII
                     TLEAALSR"
     gene            457..939
                     /locus_tag="PKZ53_05580"
     CDS             457..939
                     /locus_tag="PKZ53_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39939.1"
                     /translation="MNQTTLQHTLRWPQTLRDIPANPTMDATLITFLRTAAQLIDPLF
                     SPPAVRTDQSESTVRDDNGGYFWEGEYREGDVQHAILISYFGDQPEEMMYPQRRSEQR
                     TYTVHGLPEPFQLKLDCDYATFAGSISWIHLKLEVTGEPGMVESVQQAFVDEFNPEKS
                     "
     gene            complement(987..>3973)
                     /locus_tag="PKZ53_05585"
     CDS             complement(987..>3973)
                     /locus_tag="PKZ53_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39940.1"
                     /translation="GGGLASLDQGRWTIFTTQAGIPNNRILSLQKTPSLSGPRTLWIG
                     TDGGGLARLEFGQWVTFDTTTGLPDPVVTTVLETTSESGAPAFWIGTDAGLAHWEQNH
                     WTTYSTQTKALPDDGVITLLETGQSDGNQALWVGTSGGLARFQQGQWTTFDTSSGLPN
                     DTIWSLLETLSPAGTPILWVGTAGGGLARFEQGHWTTIEGVPNNLVTSLLETSSPDGT
                     KTLWVGTAGGGLARYVHGQWKTFDITSGLPNNTVWSLREITDSSGDKVLWIGTDSGVI
                     RLRLANLDGGWLRLQENLNQSVLNGTIHQICQDNQNRIYLSTNKGIARLSKSKTPNEE
                     FSLTTYTTEDGLPTNECNFGALVKDQSGRIWAGTVSGAVVFDPAQETEDITQKPLYIE
                     RLVAGNQNMVFPASDPFQKASLPSDQNNFVFEYSLLSYAHEGKTRYRTQLIGYDRQVS
                     DWTSNPQKEYTNLGSGSYTFQVWGKDYAGNVTGPQVVRFNIRPAPWNTWWAYCLYAGL
                     LCGVGYGGVQLRFKVLHHRNLLLEQKIAERTLELDQTNAELAENLNQLKRAKQETEYK
                     NLELDLKNAELAENLDQLSRAKKEVEQKNEELARKNLELIESQRQANLIFSALADVLP
                     GTILDRKYRLEEKIGTGGFGAVYRATQLNLGRSVAVKVFRPVPGNESLENLERFRLEG
                     VSACRVNHPNAVLILDSGVSPEGIAYLVMELLQGHSLTKELRNKYRLSLKRCAEIIIP
                     VCNVLAEAHRAGIIHRDIKPDNIFLHQSEEGEVVKVVDFGIAKFIGEQDAMQANTLTE
                     AGTIIGTPTYMAPERLGCNPYDWKSDVYSVGVMLFEMLAGQLPFVPDKEGLLNLALKH
                     MTQAPPSLCRLNPEIPVDVEAVVEQALLKNPQSRPTAKELAHHFAAAVCNHLTEDTSF
                     EGTFDVSVLLSSNPNLRLNRTLTEESPLNPRDQVTEKHYAPEQTPHGNFAATLRVDGP
                     TRKMADEFTMISEEPTGEFEKN"
BASE COUNT          898 a         1026 c         1017 g         1032 t
ORIGIN      
        1 cccgactcag tgccgcttca agcgtgatga tcaaattgaa cccgtcaatc acaatgactt
       61 gattgtgcac ggcttcaagt ggtagttgtt tggtatcccg caattgtaga agatggtccg
      121 gacaggcagt tcttgacagc gccagacgtt gacgttccct gagcgaatgg cggtctccga
      181 ccagtttcaa cgacgaattc aattgatatc cgcgactgag cagccacgaa agttcggcgg
      241 ttgctaaccg gagccgttcc acctggtctg gagcgaacaa tgtatggtct tccggatggg
      301 caccgcgatg ggttcgattg tcaggagaca taaaaatgtg gagtgcggtg gcttgacgcc
      361 gcttttgttt gaagaacgtt cattcattca ctggattcat aagggatttg gaaaagcggc
      421 gtcaagggag ccgcactctg aagaaaggac aggtcaatga accagactac tcttcagcac
      481 accctcaggt ggcctcaaac gctgcgcgat attcccgcaa atcccacgat ggatgccacg
      541 ctgattacct ttctccgaac cgccgcccaa ttgattgacc cgttgttttc accacctgcc
      601 gttcggactg accagagtga gtcaaccgtc cgagatgaca acggcgggta tttttgggaa
      661 ggggagtacc gggaaggcga cgtgcaacac gcgattttga tttcatattt cggagaccag
      721 cccgaagaaa tgatgtaccc gcaacggcgt tcagaacagc gaacttatac tgtgcacggg
      781 cttccagaac cctttcaact gaaacttgac tgcgactatg ccacctttgc cggttcgata
      841 agctggattc atctcaagct ggaagtgact ggtgagcctg ggatggttga gtctgtgcaa
      901 caggcttttg tggatgaatt taacccggaa aaaagttgac ctgatctgta gtttcaggca
      961 ctcggagtcg ccgagacttc cagcccttaa tttttttcaa attctccggt tggttcttcg
     1021 gaaatcatgg tgaactcatc agccattttt cgggttgggc cgtcaactct caaggttgcc
     1081 gcaaagtttc catgaggcgt ttgttccgga gcgtagtgct tttcagtgac ctggtcgcga
     1141 gggttcaacg gtgattcttc tgtgagcgtc cggttcagtc tcaagtttgg attactgctg
     1201 agtaacactg acacatcaaa tgtcccttca aatgaagtat cttcggtcag gtgattgcag
     1261 acggcggcgg caaaatggtg agccagttct ttggcggttg ggcggctttg ggggtttttc
     1321 aacaacgcct gctccaccac ggcttcgaca tcaactggaa tctctggatt gagccgacat
     1381 aaagatggcg gcgcctgggt catgtgtttt aaggccagat tcaacaagcc ttccttatcc
     1441 ggcacaaacg gtaattgccc ggccagcatt tcaaacaaca tcacgccgac gctgtacacg
     1501 tctgatttcc agtcatatgg attgcatccg agtcgttcgg gcgccatata ggttggggtt
     1561 ccaatgatag ttccagcctc ggtgagggta ttggcctgca tggcgtcttg ttcgccaata
     1621 aatttggcaa tcccaaagtc aaccactttg acaacttcgc cttcctccga ctggtggagg
     1681 aaaatattgt ccggtttaat atcgcgatga atgataccgg cgcgatgggc ttcggctaaa
     1741 acattacaaa ccggaataat gatttcagca caccgtttga gtgacaatcg gtatttgttt
     1801 ctcaattctt tggtaagcga atggccttgc agcagctcca tgaccaggta ggcaatgcct
     1861 tccggtgaaa cgccggaatc aaggatcaaa accgcatttg gatggttcac gcgacaggcc
     1921 gagacacctt caagccgaaa tcgctccagg ttttcaagcg attcgtttcc aggaactggt
     1981 cgaaatactt taactgccac tgatcggcca aggttgagct gagtggcgcg gtagacggcc
     2041 ccgaatccgc cagtgccaat tttttcttca agccggtatt tgcgatccag aatcgttccg
     2101 ggcaggacat cagccagggc tgaaaagatc aaattcgcct gccgctgaga ttcaatcagt
     2161 tcgaggtttt tgcgggccag ttcttcgttt ttctgttcaa cttctttttt ggcacggctt
     2221 aactgatcca gattttcagc cagttcggca tttttgaggt caagttccag gtttttatat
     2281 tcggtttcct gtttggctct tttgagctga ttcaagtttt cagccagttc agcattggtc
     2341 tggtcaagtt cgagggtgcg ctccgcgatt ttttgttcaa gcagtaaatt ccgatgatgg
     2401 agcactttaa accgtagctg tacccctccg tacccaactc cgcacagtaa cccggcatag
     2461 agacagtatg cccaccaggt attccagggc gctggccgaa tgttaaaccg gacgacctgt
     2521 ggcccggtga catttccagc atagtctttg ccccacactt gaaaggtata tgacccagag
     2581 ccgagattgg tgtattcctt ttgtgggttt gaggtccagt ccgatacctg tcggtcatat
     2641 ccaatcaatt gcgtgcgata gcgggttttt ccctcgtggg catagctgag cagcgaatac
     2701 tcaaacacaa aattgttctg gtcagagggc agcgacgcct tttgaaatgg gtcggaagcc
     2761 ggaaacacca tattctggtt gccagccacc agccgctcaa tatagagcgg cttttgggtg
     2821 atatcttcgg tttcctgggc cggatcaaac acaacggcac ctgacaccgt ccccgcccaa
     2881 attctgccag actggtcttt gaccagggcc ccaaaattac actcattggt cggaagccca
     2941 tcttccgttg tataggtggt gaggctaaat tcctcattcg gagtttttga ttttgaaagt
     3001 cgggcaattc ctttattggt tgagagataa attcgattct ggttgtcctg gcagatttga
     3061 tgaattgtac cgttcagtac agattgattc aggttctctt gaagcctgag ccagcctcca
     3121 tccagattgg cgagccgcaa ccgtatgacc ccactgtcag ttccaatcca aagtacttta
     3181 tcaccagatg aatcagtgat ttcccgcagg ctccatactg tattgtttgg caatccagag
     3241 gtgatatcaa acgttttcca ttgcccgtga acataccggg ccagtccgcc gccagccgtt
     3301 ccaacccaca gcgttttggt tccatcgggg gaacttgttt ccaacaggct ggtcaccaga
     3361 ttattgggta ccccttcaat tgtggtccag tgaccttgct caaagcgggc cagtccgccc
     3421 ccagccgttc ccacccacag gatgggcgtt cctgccggtg aaagggtttc gagcagactc
     3481 cagatggtgt cgttgggtaa gccggaactg gtatcaaacg tcgtccactg accctgttga
     3541 aatcgagcca atccgccgct ggtccctacc cacagggcct gattcccgtc agattgaccg
     3601 gtttcaagca gcgtaatcac accatcatcc ggaagggctt tcgtttgggt tgagtaggtt
     3661 gtccagtggt tttgttccca atgggccagc ccggcatcag tcccaatcca gaaggccggc
     3721 gcgcccgatt cactggttgt ttccaatacc gtggtcacca ccgggtccgg gagtccagtt
     3781 gtggtgtcaa atgtcaccca ttgcccaaat tcaagtcgtg ccaggccacc gccatccgtt
     3841 ccaatccata aggttcgagg gccagagagt gacggtgttt tctgtaaaga cagaatccga
     3901 ttatttggaa ttccagcctg cgtggtgaag atcgtccacc gaccctggtc caggctcgcc
     3961 aatcctccac cat
//
LOCUS       DAOQDO010000268         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15978_length_3972_cov_3.486852, whole genome shotgun sequence.
ACCESSION   DAOQDO010000268 DAOQDO010000000
VERSION     DAOQDO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15978_length_3972_cov_3.486852"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..2151)
                     /locus_tag="PKZ53_05590"
     CDS             complement(328..2151)
                     /locus_tag="PKZ53_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNJ39941.1"
                     /translation="MKTNFPPMPPLSGYESPAAKEIKSLAASTAAAPPYLLTHCERTN
                     YEQSGRYGEVFRYALRMKKLSPFFKSWSFGKSGQDRDLMVFCVSEDKAFKPDLARKSD
                     KLVVLIQNGIHPGEISGKDASFMLIRDILVSKKYAHWLENLTLLFIPVFNVDGHERLS
                     PFNRINQNGPREMGFRANAQRYNLNRDYIKADTPEMRAWLKLWNTWLPDFFMDNHVTD
                     GLDHQYDVTICMPTEQEIWPTVGAWTKDQFLPAVMQGMEDDGHIIGFYYEPMNRLDLS
                     EGVEGGPNQARYSNGYVATHHRPGLLVETHSLKTHRTQTWAHYDLMVNTLEYLSSHAR
                     ELKQAVQAADAEISQLGSTYHPSNKLFMAGVTSDEGEPIILKGIESSIEEGEISGAPY
                     VVYGSEPVDIPSRFYNKITTVAAPSIPLGYIVPPQWRQIIELLRLHGVETKVLNQPVS
                     DVFESYVFSNVSWAPQPFEGRLLVSFAAQLMMEKRVMPRGSVYVPMNQRAARVAMNLL
                     EPEACDSAVKWGFFNPIFEQKEDFEAYVMEPIAQRMCELDPELLDEFEARLEEDEAFA
                     ADPKARLTFFYERSGYLEPDMNQYPVVRVLRPLQLSGDSTD"
     gene            2539..>3972
                     /locus_tag="PKZ53_05595"
     CDS             2539..>3972
                     /locus_tag="PKZ53_05595"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNJ39942.1"
                     /translation="MINLLLSNPLLLLFLVAAIGYPLGKLTIRGTSLGIAAVLFVGIA
                     FGSLHPNLKVPSILYELGLGLFVYTLGLSNSAAFFTSLKRRGLRDNLLIASGLILAVG
                     LTIAIHKWFHLKVSLTVGLFAGSLTNTPALASVLETIRTSVPKETLTQMLTEPVVGYS
                     IAYPMGVVGMILVMLVLQKIWKIDYAAEALAQHEFQTGGEKLCNQTIRVTNPNADHQT
                     LKALEDEFPSLVFGRIKHEASQSLATPETRLHLGDLVSVIGSPESVAQVTHKLGEATT
                     VHLELDRSRYDFRRVFVSNHEVAGHRLADLNLMHQFGAIVTRIRRGDIDLIAQDNTIL
                     ELGDRVRVVADRDHLDAVSKFFGDSYRESSEVDILTFSLGLALGLLVGTIPIPVSDDI
                     KIKLGLAGGPLVIALILGKLERTGPLVWVPPFSVSQAIRQIGLVIFLAGIGTHAGYSF
                     VSTLTSGGGIWLLLAGMMMTCSVALVVL"
BASE COUNT          956 a          955 c         1008 g         1053 t
ORIGIN      
        1 atccgaagag acgatctcaa aacaccaatt gatagttttt cagaaaaaga ttcctctggg
       61 aacgccagca tcctgccgac gctcccagga acaggaacta ggtggaaggc tggtaaccca
      121 gggggcaacc ctcggaaaaa ttaccccttc acctccggcc tgaccagccg ctgccgtagg
      181 atgcgggtcg tgttcaaatc tgggtgaaag tctcttttct gatttgccag tatttgctgc
      241 aaatacttca ttttattaat cttcttgaga cttgcacctg tctgactttt tgaacttcaa
      301 ccgttttaaa acactatggt gccggtttta gtctgtggag tctcctgaga gttgtaatgg
      361 tcgcaggacc ctgaccactg gatactggtt catatctggc tcaagatagc cagagcgttc
      421 ataaaagaaa gtcaatctgg cttttggatc ggcggcaaac gcttcgtctt cttcaagccg
      481 ggcttcaaat tcatccagga gttccggatc cagttcgcac atgcgttggg cgatgggctc
      541 catcacatac gcttcaaaat cctctttttg ctcaaaaatt ggattgaaaa agccccattt
      601 gacggcggaa tcacaggctt caggctccag caggttcatt gcaacccggg ccgcccgttg
      661 attcattggt acatacactg atccacgtgg cataacccgt ttttccatca tcagttgggc
      721 tgcaaaactg accaggagtc tgccttcaaa tggctgaggt gcccaactga catttgaaaa
      781 gacatagctt tcaaacacat ctgaaaccgg ctgattcaag actttggttt caaccccatg
      841 cagtctgagc agctcaataa tttgtcgcca ctgtggaggc acgatatatc ccagcggaat
      901 gcttggcgcc gcaaccgtcg taattttgtt atagaaccgg gatggaatat ccaccggttc
      961 tgacccatag acgacataag gggcaccgga aatctcgccc tcttcgattg aagattcaat
     1021 cccttttaga ataatcggct caccttcatc gctggttacg ccagccataa acagtttatt
     1081 tgaagggtga tacgttgagc ccagttgtga tatttctgca tctgctgcct gaactgcctg
     1141 tttgagttca cgggcgtggg aagacaggta ttcgagtgta ttgaccatca ggtcataatg
     1201 tgcccaggtc tgggtgcggt gtgttttcag gctatgggtt tcaaccagga gtcctggacg
     1261 gtggtgagtg gcgacatagc catttgaata gcgggcctga ttggggccac cctcaacccc
     1321 ttcgctcaga tccaggcggt tcatcggttc ataataaaat ccaataatgt ggccatcgtc
     1381 ttccattccc tgcataacag caggcagaaa ctggtctttg gtccaggcgc cgacggtcgg
     1441 ccagatttcc tgttccgttg gcatacaaat cgtgacgtca tactgatgat cgagtccatc
     1501 ggtgacgtgg ttatccataa aaaagtcagg cagccaggta ttccacaatt tcaaccaggc
     1561 acgcatttcg ggtgtgtcgg ctttgatata gtctcgattg aggttatagc gctgggcatt
     1621 ggcccggaat cccatttccc gtgggccatt ctggttaatt cgattaaaag gggataaccg
     1681 ctcatgccca tctacattaa aaactggaat aaacagcagt gtcaggtttt ccagccagtg
     1741 agcatatttt ttactgacca gaatatcccg gatcagcata aaggatgcgt ctttcccgga
     1801 gatttcacca gggtggatcc cattttgaat caaaaccacc agtttgtccg attttcgggc
     1861 cagatcaggt ttaaaagcct tgtcctcaga aacacaaaaa accatcaagt cacgatcctg
     1921 ccctgatttt ccaaaagacc aggatttgaa aaaaggagag agttttttca tccgcaatgc
     1981 atatcggaaa acttcgccat agcggccaga ctgttcgtaa ttggttcgtt cacaatgggt
     2041 aaggaggtaa gggggcgctg ccgccgtgct ggcagccagt gatttgattt cttttgcagc
     2101 gggtgattca taacccgaaa ggggaggcat gggaggaaag tttgttttca taggcagtat
     2161 tcaattggga atcggggaaa taaagggttt ggttgccagc agggtgtaat ccattggcct
     2221 gaaaagtcaa cccctgtcaa aaaaattcag cccgattgta gccgctggtt gccagaaaca
     2281 gccattcact ttgaccgaaa agtgacgaaa ggttctcaaa aaacctccag tttgcaggct
     2341 tctgataatt gaccagggga aaaatcaaat tgtgataaaa tcaggagtag ggactttatc
     2401 gtgaatttta accagatgcg ttgaacctca gccgtatgtc cgtgaaatgc tggtggtact
     2461 ggtgtcggat ggactcagtc aactacattc caccaatcag aaacactaaa ctcctatgaa
     2521 aagagacttt ttggctcaat gatcaatttg ttgctttcaa atccactcct attgttgttc
     2581 ctggtggcag ccattgggta cccacttggc aaactgacga ttcggggcac cagtttgggg
     2641 attgccgccg tgttgtttgt tgggattgcc tttggctcgt tgcaccccaa tctgaaagtg
     2701 ccttcgattt tgtatgaact tggcctgggt ctttttgtgt acacgttggg gctgagcaac
     2761 agcgccgctt tttttacttc gctcaaacgt cgcggattgc gtgataattt gttgattgcc
     2821 agcgggctga tccttgccgt tggactcact attgccatcc ataagtggtt tcacttaaag
     2881 gtatcactga cggtcggact ctttgccggc agcctgacca acactcctgc cctggccagc
     2941 gtccttgaaa ccattcgaac ctcagtcccg aaagaaaccc tgacccagat gttgaccgaa
     3001 ccggtggtcg ggtactcgat tgcctatccg atgggggtgg tgggcatgat tttggtgatg
     3061 ctggtgctgc aaaaaatatg gaaaattgat tatgccgccg aagccttagc ccagcatgaa
     3121 ttccaaaccg gcggtgaaaa actctgcaat cagaccatcc gggtgaccaa cccaaacgcg
     3181 gaccatcaaa cgctcaaggc actggaagat gagtttccca gtctggtttt tggtcgaatc
     3241 aaacacgaag cttcccaatc gctggcaaca cctgaaactc gactccatct gggtgattta
     3301 gtcagcgtga ttggcagccc agagagtgtg gcgcaggtca cgcacaaact gggcgaagcc
     3361 accaccgttc atctcgaact ggatcgaagt cgctatgatt ttcgacgggt gtttgtctcc
     3421 aatcacgaag ttgccggtca ccggctggct gatttgaacc tgatgcacca gtttggggcg
     3481 attgtaaccc gcatccgccg gggtgatatt gatttgattg cccaggataa taccatttta
     3541 gagcttggtg accgggtgcg ggtcgttgct gaccgggatc accttgatgc cgtgtcaaaa
     3601 ttttttgggg actcgtaccg cgaatcaagc gaagttgata ttttgacgtt tagccttgga
     3661 ctggccctgg ggttactggt tggcacgatt ccgattccag tcagcgatga tatcaaaatc
     3721 aaacttggtc tggccggagg cccactggtc atcgcactca ttttgggaaa actggagcgc
     3781 accgggccgc tggtgtgggt gccgcccttt agcgtcagcc aggcaattcg tcaaattgga
     3841 ctggtcattt ttctggctgg cattggaacc catgccgggt attcctttgt ctcaacgttg
     3901 accagtggcg gtggtatctg gctattgctg gctgggatga tgatgacgtg tagtgtggca
     3961 ttggtcgtac tg
//
LOCUS       DAOQDO010000269         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_15980_length_3972_cov_3.208833, whole genome shotgun sequence.
ACCESSION   DAOQDO010000269 DAOQDO010000000
VERSION     DAOQDO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15980_length_3972_cov_3.208833"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKZ53_05600"
     CDS             <1..556
                     /locus_tag="PKZ53_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ39943.1"
                     /translation="NYFPIFNRKGLIVDVRHNRGGNIDSWILGRLLRKVWFNWQPRTG
                     DTYHNMQYAFRGHLVVLCDEATASDGEAFAEGFRRLGLGKVIGTRTWGGEVWLTSSNV
                     LVDNGIASAAEFGVFGPEGDWLIEGVGVVPDETVDNLPHATFKGQDTQLEAAIKHLQE
                     LIRTKPVNVLPKHPAYPDKSFRRK"
     gene            complement(694..1491)
                     /locus_tag="PKZ53_05605"
     CDS             complement(694..1491)
                     /locus_tag="PKZ53_05605"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNJ39944.1"
                     /translation="MDELEIQEYFVVGAQARDLLLHYVYNLPMNRLTRDVDVAIAVQT
                     WEQYSQLRETLINSGQFAATPKIHHRLSFIEANLPIDLVPFGDVENPPGSIRWPPDQT
                     IQMNTMGFHEAYQHAITIQISPQTVLKVASLPGLTLLKLIAWSDRGNQSTKDAEDIGL
                     LMKHYLDAGNFDRMNEKYPDLFEDPDFDLDRASNRMLGRDVRLLLNTPAIYQQVVQIL
                     SQELDQQGQLKLASVLSRYERSLEGNGFEKALSMLENLYQGIIDPIL"
     gene            complement(1538..2596)
                     /locus_tag="PKZ53_05610"
     CDS             complement(1538..2596)
                     /locus_tag="PKZ53_05610"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNJ39945.1"
                     /translation="MLRSYQAKEELLLTQVALSLTQTTGLQVVDMGQEILHSKTRWDA
                     EVTLTDGTTQWKYLVEVKSLLTSNALEHLIYQLTKYKNESHYPLLLVAPYINPKQADK
                     LRDQNIEFLDLAGNAYLKQPGLFVFITGQKPSESGKATDQKARAFNQTGLRLIFALLT
                     QPGLEQETYRVQAQRSGISLGAVAWVMNDLQKLGYLEDRGKFGRRLVQKKELLDRWVP
                     AYLEQLRPKLLFGRYQSSQPQWWEKVSLVIHVIGAFWGGEVGAEILTNYLQPATATVY
                     AETNLPRLQAQFGLRKNQLGNTEILRKFWIAPEWETKENQVAPPLVVYADLIALGDSR
                     TRETAKIIYDQYLAQLVK"
     gene            2836..>3972
                     /locus_tag="PKZ53_05615"
     CDS             2836..>3972
                     /locus_tag="PKZ53_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNJ39946.1"
                     /translation="MFQTPRIVAFSTTLTALLLIGSSAILPNTSRSHSSMNSTPSSVL
                     PGATSRDERFAEEIQAMVPELVKTRREFHMHPELSNREERTSRVVAERLKALGLEVKT
                     GVARHGVIGLLKGGKPGPVVAVRADMDALPIKETHDVPYKSLNDGVMHACGHDVHTTI
                     GLAVAELLSRHRAELAGTVKFIFQPAEEGPPRGELGGAKQMLAEGALENPRPDVIFGL
                     HCMPTIEVGKLSYNETATMASADRFFITIRGKKVHGAYPHGGIDAIVVASSAITQLQT
                     IRSRRIDTQEPLVLTIGSFHGGNRFNIVADEVQLEGTLRALNEQVRESAIQMMHQTLK
                     GVTETYGASYEMDIEKMCPVLINNTELVNQMLPTMRRIVGAENVV"
BASE COUNT          980 a          976 c          979 g         1037 t
ORIGIN      
        1 aaactacttc ccgattttta accgcaaagg actgattgtg gatgtgcgtc acaatcgagg
       61 cggaaacatt gatagctgga ttctgggccg gttgctgcgc aaagtgtggt ttaactggca
      121 gccacgaacg ggggatactt accacaacat gcagtatgcc ttccgtgggc atctggtcgt
      181 gctctgtgac gaagcgaccg cgtcagacgg ggaagcgttt gccgaaggct tccggcggct
      241 gggcctgggc aaagtgatcg gcacccgaac ctggggcgga gaagtctggc tgacctcaag
      301 caacgtgctg gtggataacg ggattgcttc ggcggctgaa tttggcgtgt ttggtccgga
      361 aggtgactgg ttgatcgaag gcgtgggcgt ggttcctgac gaaactgtgg ataatcttcc
      421 gcatgcgacc ttcaagggcc aggacaccca gctcgaagcg gcgattaaac acctgcagga
      481 gctgattcga accaaaccgg tgaatgtgct gccaaaacac ccggcctatc cggataagtc
      541 atttcggagg aagtgattag ggttcagggt tcagggtttt tgaattttgg tcctgttttg
      601 ttccttttcg tcccttttgt cttttaaacc ctgaacccta taccctatac cctacctgta
      661 tgatttggtt ttctggataa ggaagctggt tgatcacaaa atcgggtcaa taattccctg
      721 atacaggttt tcaagcattg ataaagcttt ctcgaaacca tttccttcaa gacttctctc
      781 gtatcttgaa agaacacttg ccaatttaag ctgcccctgt tgatcaagtt cttggctcag
      841 gatttggacc acttgttggt aaatcgcagg agtattcaat agcaatcgca catctctccc
      901 aagcatgcgg ttgcttgctc ggtcaagatc aaagtctgga tcctcaaaca gatccggata
      961 cttttcattc attcggtcaa aattacctgc atcaagataa tgtttcatca aaaggccaat
     1021 atcctcggca tctttggttg actgatttcc tcgatcagac caggcaatga gtttcaatag
     1081 cgtcaagcca gggagtgaag ctactttgag aaccgtttgt gggctgattt ggattgtgat
     1141 ggcgtgttga tacgcttcgt gaaagcccat ggtattcatt tggattgttt ggtcaggtgg
     1201 ccatcggata gaacctggtg gattttccac atcaccaaat ggaacaagat caattgggag
     1261 gttggcttca ataaaagaca accgatgatg aatctttggg gtggctgcaa attgacctga
     1321 attgatcaac gtctcacgaa gctgactgta ttgttcccag gtttgaactg caattgcgac
     1381 atcaacatca cgtgttaacc gattcatcgg gaggttatag acataatgca gcaacagatc
     1441 tcgtgcctgg gcaccaacca cgaaatattc ctgtatttct aactcatcca tttgctggga
     1501 caaaatctca aagaaccgaa gcaatggttc cggtaactca tttgacaagc tgagcaaggt
     1561 actggtcata aattattttt gctgtttcac gtgttcgaga atcaccaagc gcaatgagat
     1621 cagcatagac caccagtggt ggcgccacct ggttttcttt ggtttcccat tctggtgcaa
     1681 tccaaaattt ccgcaaaatt tcagtattcc caagttggtt tttgcgcaac ccaaattgtg
     1741 cttgaagtcg cggcaaattc gtttcagcat acactgttgc cgtagctggc tgcaggtagt
     1801 tagtcaggat ttccgcaccg acttcgccac cccaaaaagc accaataacg tgtatgacga
     1861 gactcacttt ttcccaccac tgtggttgag aggattggta tctgccaaac aacagtttcg
     1921 gacgaagttg ctcaaggtaa gccggaaccc atcggtcaag taactccttt ttttgaacca
     1981 gtcgtcgccc aaattttccc cgatcttcta aataaccaag tttctgaaga tcattcatca
     2041 cccaggctac tgcaccaagc gaaattcctg accgttgtgc ctgaacgcga taagtttctt
     2101 gttctaatcc gggttgggtc agcaaagcaa aaatcaatcg cagcccggtt tggttgaaag
     2161 cgcgtgcttt ctggtcagtt gcttttcccg attcgctcgg tttttgtccg gttatgaaga
     2221 caaacagccc tggctgtttt aaatatgcgt ttccggctaa atccagaaac tcaatgttct
     2281 gatctctgag tttgtctgcc tgtttgggat tgatataagg cgcaaccagg agcaatgggt
     2341 agtgggattc atttttatac ttcgtcagtt gatatatcag gtgttcaagg gcgttgctgg
     2401 taagaagcga tttcacttca accaggtatt tccattgggt ggttccatca gtcaatgtca
     2461 cttcggcatc ccaacgagtt ttcgaatgca agatttcttg ccccatgtca accacctgta
     2521 acccggtagt ttgagttaaa ctcaaagcga cctgtgtaag aagcaattct tctttagcct
     2581 gatatgagcg aagcacaaaa cctacctcca gaattcaatt tcagagttgt tgttcacatt
     2641 ctagcaatgt tcataatttg tgaacagaca aaaacgtgaa caaggcaaga ttctatagaa
     2701 agaaatgctc atcagagttg tcaatcgtgg atagctctgg tttgattgca agcactcacc
     2761 ctgagacatt tcttgaccat tggtttgaag gtcgaataat ccatctggct atttgcttga
     2821 aatcgtctac tcgctatgtt tcaaactcca cgaattgttg ctttttccac cactctgact
     2881 gcgttgctcc tgattggatc cagcgccatc cttccaaata catccaggtc acactcatct
     2941 atgaattcga ctccttcttc tgttctgccc ggtgcgacca gccgtgacga gcgatttgcc
     3001 gaagaaatcc aggccatggt gccggaactg gtcaaaacgc ggcgcgaatt ccatatgcat
     3061 cctgagcttt ccaaccgcga agaacgcacc tcccgcgtgg ttgctgaacg gctcaaagca
     3121 cttggcctcg aagtcaaaac cggcgttgcc cgccatggcg tgattgggtt gctcaaaggt
     3181 ggcaagccag ggccggttgt tgcggttcgc gccgatatgg acgcacttcc aatcaaagaa
     3241 acccacgatg taccctataa atcgctcaat gacggcgtga tgcacgcctg cggacacgat
     3301 gttcacacca caattgggct ggccgtggcc gaacttctgt caagacatcg ggcggaactc
     3361 gctggaaccg tgaagtttat cttccagcct gccgaagaag gtccaccccg tggcgaactt
     3421 ggcggcgcca aacaaatgct ggccgagggg gcgcttgaaa atccgcgtcc cgatgtgatt
     3481 ttcgggctgc actgtatgcc gacgattgag gtcgggaagc tttcctacaa cgagacggcc
     3541 accatggcga gcgcggaccg gtttttcatc acgattcgcg gaaagaaagt ccacggtgcc
     3601 tacccacacg gcggaattga cgcgattgtg gtggcttcat cggcgattac gcagcttcag
     3661 accattcgca gccggcggat tgatacccag gaaccgctcg tgttgacgat tggcagtttc
     3721 cacggtggca accggttcaa catcgtggct gacgaagtac agcttgaagg cacgctccgg
     3781 gcgctcaacg aacaggtccg ggaaagcgca atccagatga tgcaccagac actcaagggc
     3841 gtgactgaaa cctacggcgc gtcctatgaa atggacatcg aaaaaatgtg cccggtgctc
     3901 attaacaaca ccgagctggt caaccagatg cttccgacca tgcggcgcat tgtcggcgct
     3961 gaaaacgtcg tc
//
LOCUS       DAOQDO010000270         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16001_length_3968_cov_3.519295, whole genome shotgun sequence.
ACCESSION   DAOQDO010000270 DAOQDO010000000
VERSION     DAOQDO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16001_length_3968_cov_3.519295"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1315
                     /locus_tag="PKZ53_05620"
     CDS             302..1315
                     /locus_tag="PKZ53_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNJ39947.1"
                     /translation="MLSTKSPFAFSFFILGLLGFITSSLISCGGANPTAETSSPVSQP
                     PTTDMEKPFKVEFDAERAFEHVRKQVEFGPRPAGSAAGKQTRDYLVDQLKKAGLKVTL
                     DSFKTGTPLIRMPSVEMHNVIGELPGERDDIIVIASHYDTKYFENFRFVGANDGASST
                     GVVLELARVLATLAPADRKISHTLWFVFFDGEEAFASWSGTDHTYGSRHLVTKLKADG
                     KLSKVKGLILLDMVGDKDLVIPREMQSSPWMADAMVLSAQQLGYGAHFPRTTHYIDDD
                     HIPFLQAGIPAVDLIDFEYGGPDNPYWHTEEDTLDKISPKSLKIVGDTVLHALPKIAA
                     QAR"
     gene            1436..2494
                     /locus_tag="PKZ53_05625"
     CDS             1436..2494
                     /locus_tag="PKZ53_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNJ39948.1"
                     /translation="MTYQKIAVIPGDGIGVEVTREALHVLECVTEKFGLPLVFESFDW
                     GADKYLAEGVTLPEGAVEMFERDFAAIFTGAFGDPRVPDNRHAADILLGLRFKLDLYI
                     NLRPIVLLNDWLTPLKGRVLQDLNFVIFRENTEGLYVNAGGVLKRGTADEVAIQEEIN
                     TRRGVERIIVAAFDYARNHGRTRVTLADKSNLLTYGHDLWRRTFREVALRYPEIDANA
                     QYIDAMAMLMITNPEQYQVIVTNNLFGDILTDLGAGLVGGLGLAASGNIHPGRVSLFE
                     PVHGSAPTIAGKNIANPFGAILTAAMMLEYLGHLDASQAVEHAVIDCIARNEVTVDIG
                     GTLSTSEAGEAVLNLLRN"
     gene            complement(2684..3175)
                     /locus_tag="PKZ53_05630"
     CDS             complement(2684..3175)
                     /locus_tag="PKZ53_05630"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNJ39949.1"
                     /translation="MLNIVLVEPEIHTNTGNISRTCVCTGASLHLVRPLGFSVDSKQL
                     RRAGLDYWDALNVQYYDNFAQVQETFPQGRFFFASTKARRTYADVSYQAGDFLVFGRE
                     TRGLPEELIEANLETAIRIPMLETARSLNLSNAASIVLYEALRQLGFPNLLTAGQLSK
                     TLG"
     gene            3699..>3968
                     /locus_tag="PKZ53_05635"
     CDS             3699..>3968
                     /locus_tag="PKZ53_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39950.1"
                     /translation="MTQVLNSSLPQENMLAIRSETNKRDNNEDSFVVLSFHIGIDGPP
                     VTELAVADGMGGYAHGEVISRDALKKYATTLFQNLAVEHCLNVISP"
BASE COUNT         1029 a          945 c         1000 g          994 t
ORIGIN      
        1 aaaaaattgg cagttttttt tgcggcatcc gcaacaggct gaccaaaaga acaccatgcc
       61 cgccgtgaca ggtctcaccg acgaagagta tcgtttgttt gccgactggc tttcaaaagt
      121 ttcgcgcacc aatcccaaat ttgaaccagg aaagaaatag ggaccgggca gaaaaaggac
      181 aaaaagacag aagggacaaa aaggacataa aatcccatct ctaaacccaa accccatttt
      241 cttcaaccct cagcccgttt tcttcagccc caagcccgtt ttcttcagcc cttgaatttt
      301 tatgctttcc accaaatcac catttgcctt ctcatttttt atattgggtt tgctgggttt
      361 tatcaccagc agcctgatca gttgtggtgg cgccaacccc acggctgaaa ccagttcacc
      421 tgtatctcaa ccaccgacga ctgacatgga aaaaccgttc aaagtggaat ttgatgccga
      481 gcgagcgttt gaacacgttc ggaaacaggt cgaatttgga ccccgtccgg ctggatcggc
      541 ggctggcaaa caaacccgcg actacctggt tgaccagctc aaaaaagccg ggttaaaggt
      601 gacgcttgac tcatttaaaa ccgggacgcc actgattcgc atgccctcgg tcgaaatgca
      661 caatgtgatt ggcgagcttc ctggcgaacg tgacgacatc atcgtgattg ccagccacta
      721 tgacaccaaa tattttgaga attttcggtt tgtcggcgcc aatgatgggg cttcgagtac
      781 cggcgtggtg cttgaactgg cacgggttct cgccacttta gcccctgcgg accgcaagat
      841 ctcgcacacg ctgtggtttg tgttctttga cggcgaagaa gcctttgcaa gctggtctgg
      901 gaccgaccat acctatggtt cacggcatct ggtcacaaaa ttaaaagccg atgggaagct
      961 ttcaaaagta aagggtctga ttttgctgga tatggtggga gataaagatc tggtgattcc
     1021 tcgcgagatg cagtcttcgc cgtggatggc cgacgcgatg gtgctttcgg ctcaacaact
     1081 tgggtacgga gcgcattttc cacgaacaac gcactacatt gacgacgacc acattccgtt
     1141 tttgcaagcc ggtattcctg ctgttgatct gattgatttt gaatatggcg gacctgacaa
     1201 tccatactgg catacggagg aagacacgct ggataaaatc agtccaaaaa gcctgaagat
     1261 tgttggtgac acggtgctgc atgccttgcc gaagattgct gcccaggcac gatgacagag
     1321 tgacaaggtg atacaagaac agccagaaac gatgtaaggg tgctgttgcc ttgaaccgca
     1381 cccaattcga aaaccctgaa ccctgaaccc tactgcttaa agaaggaaga aaggaatgac
     1441 ataccaaaaa attgctgtga ttcccggaga tggaatcgga gtcgaagtca cccgcgaggc
     1501 actgcatgtc ctcgaatgtg tgactgaaaa atttggctta ccgcttgttt ttgaatcgtt
     1561 tgactggggt gctgataagt atctggccga aggcgtgacc ttgcccgaag gggccgtcga
     1621 gatgtttgag cgcgactttg ccgcgatttt tacgggcgcg tttggtgatc cccgtgtccc
     1681 tgacaaccga catgcggctg atattctgct tggattgcgg ttcaagcttg atctctatat
     1741 caacctgcgc ccgattgtgt tgctcaacga ctggctgact ccgctcaaag ggcgggtgct
     1801 gcaggatctc aactttgtga tttttcggga aaacaccgag gggctttatg tcaatgccgg
     1861 cggtgtgctc aaacgtggga ctgccgacga agtcgccatc caggaagaaa tcaacacccg
     1921 gcgcggggtt gagcggatta tcgtggcggc gtttgattat gcccggaacc acggtcgaac
     1981 cagagtgacg ctggctgata aatcaaacct gttgacttac ggccatgacc tctggcgtcg
     2041 aacctttcgc gaagttgccc tgcgttaccc tgaaattgat gccaatgctc aatatattga
     2101 cgccatggcg atgttgatga tcaccaaccc agagcagtat caggtaattg tgaccaataa
     2161 tttgttcgga gacattttga ctgatcttgg ggctggattg gtcggaggtc tggggctggc
     2221 ggcatctgga aatattcatc caggccgggt ttcacttttt gaaccagttc atggtagcgc
     2281 tccgacaatt gctgggaaaa atatcgccaa tccgttcggg gcaattttga ccgctgccat
     2341 gatgcttgag tacctgggcc atcttgatgc ttcacaagcc gttgagcatg ccgtcattga
     2401 ttgtattgcc cgcaacgaag tgacggttga catcggcggc acgctcagta ccagcgaggc
     2461 tggtgaagcc gtgctgaact tgcttcgaaa ctgaaaaacc cctgggttcg gggctgaaga
     2521 catcgggctg aagacatcgg gctgaagaag tcgggctgaa gacatcgggc tgaagacatc
     2581 gggctgaaaa cattgggctg aagagttggt tttatttcat ccctcatcct tcatccctca
     2641 tcccttcgat tgccccaagc ccgatgtctt cagccctggt tttttagccc aacgtcttcg
     2701 ataattgccc tgcggtcaat aagttgggaa agccaagctg gcggagtgct tcatagagca
     2761 caatcgaggc ggcgttggag agattgagcg agcgagccgt ttcgagcatt ggaattcgaa
     2821 ttgccgtttc cagattggcc tcaatcaact cttccggcaa gcctctggtt tctctgccaa
     2881 aaaccaaaaa atcccctgcc tggtagctca catcggcata ggtgcgtctg gctttggtcg
     2941 aagcaaagaa aaaacgtcct tgtggaaagg tttcctggac ctgagcaaaa ttgtcatagt
     3001 actgcacgtt gagcgcgtcc cagtaatcca gtccagccct ccgtaactgc ttgctatcaa
     3061 ctgaaaaccc aagtggtcgt accagatgca gtgaagcacc ggtgcaaaca caggtgcgcg
     3121 aaatgtttcc ggtattggta tgaatttctg gttcaacaag aacaatgttc agcatatgaa
     3181 tggaatgaag aattgagaat gaagaatgaa gaatgaaaag tgaagaattg agaatgacct
     3241 aaattttgcc caaaagagca ccaccttgct caaagatcag tcccgaccag gtcgagatac
     3301 cactcggaaa caggaagtga ccagatcatt gagtgggatc aaagttcact caaattgagc
     3361 cttcgtggtg acaaaactga cttccagtct gtcctttttg tcctttttgt cctttgattt
     3421 tggtcttacc aggtaccaaa actggggacc aggtcagtat tttgggcgaa atttgaagaa
     3481 tgaagaatga agaatgaaga atgaagacag ataaggtatt atttgttttc cataccagtt
     3541 tgaaatcatt cttttgtgtt tggtaagaag taaattgttc ggaaaaatag gtttaccaga
     3601 aacctgacaa ttgacatctt gcgacaaact tgcaccacgt tctcaattct acatttttca
     3661 ttctacattc tgaattcttc attccaagga gaccaggcat gacacaggta ctcaattcca
     3721 gccttcctca agaaaacatg cttgctattc ggagcgaaac caacaagcgt gacaacaacg
     3781 aagacagctt tgttgtgctg tcttttcata ttggcattga tggacctcca gtcactgagc
     3841 tggctgtggc tgatggaatg ggtggatatg cccatggcga agtcatcagc cgcgacgcac
     3901 tcaaaaaata cgccacaacg ttgtttcaga acctggcggt tgagcattgt ttaaatgtta
     3961 tttcacct
//
LOCUS       DAOQDO010000271         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16095_length_3956_cov_2.999231, whole genome shotgun sequence.
ACCESSION   DAOQDO010000271 DAOQDO010000000
VERSION     DAOQDO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16095_length_3956_cov_2.999231"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKZ53_05640"
     CDS             complement(<1..439)
                     /locus_tag="PKZ53_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ39951.1"
                     /translation="MTHNKILIVDDEKRQREILELILTSEGYRANVAASGEAALRMVK
                     NEHFDLVLTDLKMGGMSGLELMKNLTQLDSSIIVILMTAHGSIDSAKEAIRHGAFDYL
                     EKPLDQVRLLAVISQALGRLNTLDGEIIGQSEQMERLKKLILKV"
     gene            complement(476..1957)
                     /locus_tag="PKZ53_05645"
     CDS             complement(476..1957)
                     /locus_tag="PKZ53_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ39952.1"
                     /translation="MSVYLGLRFRLIVLMTTLLVVTVGVLYELNRRSEEQIRRQVSEQ
                     IKELTAAVDVGLKSISDVNYLEVIRQRDLPAEQYNRIKHIMITDSEGRVTDAANSNER
                     GLKILIPNPGSSLVVAGDPLETDSTQATLHQTEEAKQTIFIPFRAAGVDGKPEINYVV
                     VVMSSLRLDETLADTSRNRLLATIVVLLVGLLTSVGLVWRFTSPLGQLIKAVQRVADG
                     DLQFHIGLRRKDEVGQLAANFDQMIDQLRGKQELEERLNQAERAAVIGRLASGIAHEI
                     RNPLNFINLTIDHVRTKFPPTEAAGRALFDRLLISVKEEIARLNSLVNNVLRFGRPAR
                     LARKSISLRTIVESVFQVVQTQAQEQGVELKIDDQTGGQLIEVDAELMSSCLSNLAIN
                     GIQAMPEGGLLTVKLQTEPDGQVITVTDRGVGIPSDKLDQIFEPYYSTKETGIGLGLA
                     VTRKLIEEHNGSILVRSTVGVGTTFVIRLPLSILEHSASSTGA"
     gene            2351..3601
                     /locus_tag="PKZ53_05650"
     CDS             2351..3601
                     /locus_tag="PKZ53_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ39953.1"
                     /translation="MPYQLFIAWRYLRARRSQKVLSIISAISALGITVGVWALVSVLA
                     FQSGMEHELRTKILGGTSHLNLLRKADLPIENPSALIEKIAQIRHVKSAAATSYHTVL
                     LSGPQHPAMGILKSVDLTGSVQGIELYQTLIPGGNIGHLRRTQYPDGSEFPGVILGKQ
                     LAEDLNVRVGEVLQIVTPGDKGDLTPVGILPRSFTLKVVGLFESGLYEYDSAWGYIAL
                     ETAQELTGEAQSARVIQIMLDDIYQVKEVAAAVQIIAGPEFVTNDWQELNQPAFAALN
                     LQRLGFAVGISLIILVAALNIVTTLIMLVVEKKRDIAILTAMGATSGDILVIFMIQGV
                     VIGAVGTLVGGMCGAVTAIVGDKYRLIQLDPRTYSISYVPFRFSASDTVLVLLIAMGI
                     SFFATLYPAWQASRLDPVEGIRQG"
     gene            complement(3756..>3956)
                     /locus_tag="PKZ53_05655"
     CDS             complement(3756..>3956)
                     /locus_tag="PKZ53_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39954.1"
                     /translation="VRSPEGVTVTAFLMGDLAGGSSLIVGDTLNHRVQGRFLAGGAWQ
                     LVGVPNNAGSLIGQFRSLSKIR"
BASE COUNT          931 a         1032 c          989 g         1004 t
ORIGIN      
        1 ccactttgag aatcaacttt ttcagccgtt ccatctgctc gctctgcccg atgatttcgc
       61 catccagtgt attgagtcgc ccgagggcct gcgaaatgac agccagcaat cgaacctgat
      121 ccagcggttt ttcaagatag tcaaacgcac catggcggat ggcctccttg gccgaatcaa
      181 ttgatccatg ggcggtcatc aagatcacaa tgatggatga atcaagctgg gtcaggtttt
      241 tcattaactc aaggccgctc atccccccca ttttcagatc cgtcaggacc agatcaaagt
      301 gctcattttt gaccattctg agcgcagcct caccactggc agcgacgttg gcgcgatatc
      361 cttctgaggt caaaatcaat tccagaatct cgcgctggcg tttttcatca tcaacaatca
      421 ggattttgtt atgggtcaca ggtcactcct ttgttcgttc accgcacagc cagtgtcaag
      481 cccctgtcga ggaggcagaa tgttcaagga tgctcagggg aaggcgaatc acaaatgttg
      541 ttcctacccc aaccgtactt ctcaccaaaa tagaaccatt gtgctcttcg atcagtttcc
      601 gggtaaccgc caggccgagc ccaattccgg tctctttcgt agagtagtaa ggctcaaata
      661 tctggtcaag cttgtcggag ggaatcccga ctcccctgtc agttaccgta atcacctgcc
      721 catccggctc agtttgtagc ttgaccgtga ggagacctcc ctcgggcatc gcctggatcc
      781 cgttgatcgc caggttggac agacacgaac tcatcaattc cgcatcaacc tcaatcaatt
      841 gcccgccagt ttggtcatca attttgagtt caaccccctg ttcctgagcc tgagtttgaa
      901 caacctgaaa cacactctca acaatggtgc gaagcgaaat tgattttctg gccagccgcg
      961 cgggccggcc aaaccgcaga acgttgttga ccagtgaatt taagcgggca atttcttctt
     1021 tgaccgagat caaaagccgg tcaaataaag cacggccagc ggcttcagtc ggaggaaatt
     1081 tggtgcggac gtgatcaatc gtcaggttga taaagttcaa tgggttgcga atctcatggg
     1141 caatccccga agccagacgc ccaatcacag ccgcccgttc tgcctggttt aaccgttcct
     1201 ccagttcctg ctttccccgc aactgatcaa tcatctggtc aaagttagct gccagttgac
     1261 caacttcgtc tttgcgccgc aggccaatat gaaattgaag gtcaccgtcg gccactcgct
     1321 gaacggcttt gattaactgt ccaagcggac tggtaaagcg ccagaccagc ccaaccgagg
     1381 tcagaagtcc aacaagcaac acgacaatcg tggccagtaa ccggttgcgc gaagtatcag
     1441 ccagggtttc atccaatcga agcgatgaca tcaccaccac cacgtagtta atctcgggct
     1501 tgccatcaac acccgccgcc cggaaaggaa taaaaatggt ctgcttggct tcttctgttt
     1561 gatgcagtgt ggcctgagtt gagtcagttt cgagcgggtc accagccaca acgagcgaac
     1621 tcccagggtt tgggatcagg atttttaaac cgcgttcgtt gctgttggcc gcatcagtga
     1681 cccttccttc cgaatcggta atcataatgt gtttaatgcg gttgtactgc tcagccggaa
     1741 gatcgcgctg gcgaattacc tccaggtaat tgacgtcgct gatgctcttg aggccaacat
     1801 ccacggcggc ggtcagttct ttgatttgct cggaaacctg gcggcgaatc tgctcttccg
     1861 aacgccggtt cagttcatat aaaaccccga cggtcaccac cagtaatgtg gtcatgagca
     1921 caattaagcg aaaacggaga ccaaggtaga ctgacacgcc atctcctcct gtgtgatgag
     1981 aacgatttga tgtgggcagg atgctgactt caagagtgct tggctctgac tgatgacctg
     2041 ggctatgctg tggtcagagt ggatgctttt ttcccaaacg ataaaggcac gtatctcttg
     2101 ataacactgg tgttgtgtct gatgttcagt ggttatacaa gttggccggc aacttcaatt
     2161 cattcagatc aaagcatcat tgtcaactca gggcgacact caatcatcca ggtgtttcat
     2221 cattcttgga accaaaccca ggaattatgc tctgtttcaa aaacgtcgct tcactattca
     2281 tcatttcata cacgaaacag ggagccagtt gaacaggcac ttcgtgtcaa ctgagaaccg
     2341 gtcttcttca atgccctatc aactctttat tgcctggcga tatttgcgcg cccggcgaag
     2401 tcaaaaagtc ctctcgatta tcagtgccat ctctgccctg ggtatcaccg ttggggtatg
     2461 ggcactggtg agtgtactgg cgtttcaaag cgggatggag cacgagttgc gaaccaaaat
     2521 cctgggtggc acctcacatt tgaacttgct gcggaaagct gatttaccga ttgaaaatcc
     2581 atcggcgctg attgagaaaa ttgctcagat ccgacatgtc aaatctgccg ctgccaccag
     2641 ctatcacacg gtgctgttgt caggccctca gcatccggcg atgggaatct tgaaatcggt
     2701 tgacctgact ggctcagtcc agggcattga gttgtaccag accctcattc caggtggaaa
     2761 tatcgggcat ctgcgtcgga cccaatatcc agatggcagt gaatttccag gggtgatcct
     2821 tggcaaacaa ctggccgaag acctcaatgt gcgggttggc gaggtactcc agattgtcac
     2881 tcccggagat aaaggtgatc tgaccccggt cgggatttta ccgcgctcgt ttaccttgaa
     2941 ggtggtgggt ctatttgaat ccggactcta tgaatatgat tcagcctggg gatatatcgc
     3001 gcttgaaacc gcccaggaac tcactggaga agcccagagt gcccgggtca tccaaatcat
     3061 gctcgatgat atttatcagg tcaaagaagt tgccgctgcc gtccaaatca ttgcgggacc
     3121 tgagtttgtc accaatgact ggcaggaact caaccagcca gcgtttgcgg ctctcaatct
     3181 gcaacggctt ggttttgcgg ttgggatcag tctgattatt ctggtagccg ccctcaatat
     3241 tgttaccacg ttgattatgc tggtggttga gaaaaaacgg gacatcgcca tcctcacggc
     3301 aatgggggcc acatcaggcg atattctggt gatttttatg attcagggag ttgtaattgg
     3361 tgcggtgggc accctggtgg gaggaatgtg cggcgcggta acggctattg ttggagataa
     3421 ataccgattg attcagcttg acccgcggac atattcgatc tcctatgttc cgtttcggtt
     3481 ctcagccagt gacacggtgc tggtactcct gattgcaatg ggaattagct tttttgcgac
     3541 actgtacccg gcctggcaag cctcacgact ggatccggtt gaaggaatca ggcagggatg
     3601 atgaataaag aatcctttga gtgattagtg gttagcaatt agtgcctggt tcctggttcc
     3661 tggttcctgg ttcttggttc ctggttcttg gttcttggaa agtattgatt tctaaccact
     3721 aactgctaac cactcaaggg cttcttcatt cctggttatc gaatcttact cagactgcgg
     3781 aactggccga tcaacgaacc cgcgttgttg ggtactccga ccagttgcca ggcgccacca
     3841 gccaggaatc gtccttgaac ccgatgattg agggtgtcac caacgataag tgatgatcca
     3901 ccagccagat cacccattaa gaaggccgtc acggttacgc cttcaggtga tcgcac
//
LOCUS       DAOQDO010000272         3950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16138_length_3950_cov_2.772529, whole genome shotgun sequence.
ACCESSION   DAOQDO010000272 DAOQDO010000000
VERSION     DAOQDO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16138_length_3950_cov_2.772529"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3950)
                     /locus_tag="PKZ53_05660"
     CDS             complement(<1..>3950)
                     /locus_tag="PKZ53_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ39955.1"
                     /translation="FDQNDVWTLFHSVAFDFSVWELWGALLYGGKLVVVDYWTSRSPE
                     DFYKLLQREQVTILNQTPSAFRQLMRVDQAELAELALRYVIFGGEKLEPVTLLDWFER
                     HGDAHPRLINMYGITETTVHVTFHPLTKADAERGQTSPIGVPIPDLQVFVLDRHGLPV
                     PIGVKGELYVGGAGVARGYLNRPELTAERFALGAEGWGLGAEDRAQGSQYMAQGSGLG
                     AESYKSPMILSPRPSALSPQPLISSPQPLAPSPMSSALSPRFYRTGDLGRWLPNGKLE
                     YLGRIDDQVKIRGFRIEPGEIQAVLNQQPEVAESLLLVKDDAHSGQRLVAYVVPKDEV
                     DTLALIHELQARLKAVLPEYMVPSAFVVLTMFPLTAHGKIDRRQLPEPEQFALPASEQ
                     IGPRTATEAALVELWTELLGLNSLSVTDNFFDLGGHSLLAAQLVSRIRDSFQVELPLR
                     RLFEIPVLEEMAAEIQSALVANKPDEAMLSAPPPIVPVSREQEFPLSFAQERLWFLHQ
                     LEPGTSAYNILFALRLTGALDQSALERAFVELIHRHEVLRTGYLISDGQPIQYIQDNN
                     LPFHIWNQDLSTELMALDEPARKRRIGQILVTEAQKPFDLTHPPMLRVGVLKLAENDH
                     ILVLTLHHIAADGWSMGILVRELGMLYRAYSKGLSPLLPPLPVQYADFAHWQRGWLQG
                     TVLEAQVAYWKEKLQDVPPVLELPTDRPRPAMQTFNGATRGFALSAEVTQSLHALSRR
                     EGVTLFMTLLAAYNVFLARYSGQADICVGTTTANRNRKEIEGLIGYFLNTLVLRTRLE
                     SGSSTQWTTFRELLGRVRETTLGAFSHQDVPFEKLVEVLHPKRELSHSPLFQVAFVLQ
                     NTPLQTVPLGDIQVQLLSGTDNTAKYDLLLSVIETADGLKCGMEYNTDLFDASTIDRM
                     ISHFRTLVENLLASPDESVSLVPMMSPAEAHQILVEWNTTAVELTEPLLAHRRFEQQV
                     AQTPEATAVEFINPQTGERTSPSYFELNQQAERLANHLQTLGVGPDVIVGLCLERSVE
                     MVVGILGILKAGGAFLPMDPTYPADRLTFMVEDAGLSVMVTDTQSTQWLSENEKVTTI
                     NLKDINSESEVRNPEPGTRNPEPEALAYVIYTSGSTGKPKGTLLTHQGLCNLSVAQQR
                     AFQVTPGDRVLQFASLSFDAAVWEVAMALLSGSTLVVTTRETVTSPEHLVRFLQTESI
                     TIATLPPTMLSAMPATNLPALRLLVVAGERCPAEVANQWSPGRRFVNAYGPTETTVCA
                     SMFECHGTLTQPPPIGRPIANTQLLIVDPWGNPVPAGIPGELYIGGTSLARGYL"
BASE COUNT          886 a         1069 c         1119 g          876 t
ORIGIN      
        1 tgagataccc acgggcgaga ctggttccgc caatgtacaa ctcaccaggg atgcctgccg
       61 ggacgggatt tccccatgga tccacaatca acaactgggt attggcaatt ggccggccaa
      121 tcggaggagg ttgggtcaag gtcccgtggc attcaaacat tgaggcacag accgttgttt
      181 ccgtcggacc ataggcgttg acaaagcgtc gcccaggtga ccactggttg gcgacttctg
      241 ccggacagcg ttccccagcc acgaccagca ggcgcagggc cgggaggttc gttgccggca
      301 tggcggaaag catcgttggc gggagggtgg caattgtaat ggattcggtt tgtaagaacc
      361 ggacaaggtg ttctggtgat gtcaccgttt cacgcgttgt cacgaccaga gtacttccac
      421 tcaagagtgc catggcgact tcccaaaccg ccgcatcaaa gctcaacgaa gcaaattgca
      481 gtacccgatc ccccggtgtt acctggaatg cccgttgctg ggcaaccgag aggttacaca
      541 gcccctgatg agtcagaagc gttcctttgg gtttcccggt cgagcctgaa gtgtagatga
      601 cataagccag agcctcgggt tccgggttcc gggttccggg ttccgggttc cggacttcag
      661 attcggaatt tatgtccttg agattgattg tggttacctt ttcattctct gataaccatt
      721 gagttgactg ggtgtcggtg accatgaccg aaagcccggc atcctcgacc ataaatgtca
      781 gccggtcagc cgggtaggtt ggatccattg gcaggaaggc accaccggct ttgaggattc
      841 ctaaaattcc aaccaccatt tcaactgacc gctcaagaca caggccgaca atgacatccg
      901 gcccaacacc aagtgtttgc agatggtttg ccagccgttc cgcttgctgg ttgagttcga
      961 aatacgaagg cgaagttcgt tctccggttt gcgggttgat aaactcaacg gcagtggctt
     1021 caggggtttg cgcaacctgt tgttcaaagc gccgatgagc cagcaatggt tcggtgagtt
     1081 cgacggcggt ggtgttccat tcaaccagaa tttggtgcgc ttcggcaggt gacatcattg
     1141 gcacaagcga taccgactcg tcaggcgagg ccagcagatt ctcgaccagc gtccggaaat
     1201 ggctgatcat ccggtcaatc gtcgaagcat caaacaggtc ggtgttgtat tccatcccgc
     1261 atttcaggcc gtcagcggtt tcaatcaccg aaagcaacaa atcatatttc gctgtattgt
     1321 cagtgccgct caagagttgt acctgtatgt ctccgagcgg tactgtctgg agcggcgtgt
     1381 tttgtagcac gaaggccacc tgaaacagcg gcgaatggct caactcgcgt ttgggatgca
     1441 ggacttcgac cagtttctca aacggaacat cctgatggct aaaggcccca agggttgttt
     1501 cccgaacccg acccaggagt tcccggaagg tcgtccactg agttgatgac cctgattcaa
     1561 gtctggtccg tagaaccagc gtgttgagaa agtatccgat taagccttcg atttccttgc
     1621 ggttccggtt agccgtggtt gtcccgacac aaatgtctgc ctgtccgcta tagcgtgcca
     1681 gaaagacgtt gtaggccgcc agcagcgtca taaacagcgt aacgccttca cgccggctca
     1741 atgcgtgcag cgattgggtg acttcagccg agagagcaaa tcctctcgtg gcgccattga
     1801 aagtctgcat cgctggccgt ggccggtcag tcggaagttc cagcacgggt ggcacatcct
     1861 gaagcttttc tttccagtag gccacctggg cttcgagcac cgttccttgc agccagccgc
     1921 gttgccagtg agcaaaatcg gcatactgca ccggcagtgg cggcagcagt ggcgacaacc
     1981 cttttgaata ggcccggtag agcatcccca gttcacgcac cagaataccc attgaccagc
     2041 catcagccgc aatgtgatgg agcgtcagca ccaggatatg gtcattttcc gccagcttga
     2101 gcaccccgac gcgcagcatt ggcggatggg tcaggtcaaa cggtttttgg gcttcggtga
     2161 ccagaatctg gccaatgcga cgtttgcgag cgggttcgtc aagcgccatg agttcagtgg
     2221 aaagatcctg gttccagatg tgaaacggca gattgttatc ctgaatgtac tgaatcggct
     2281 ggccatcgct gatcaaataa ccggtgcgca aaacttcgtg acggtggata agttcaacaa
     2341 atgcgcgttc cagtgctgat tgatcaagcg cacctgtcag ccgaagtgca aacagaatgt
     2401 tataagctga tgtgcctggt tcaagttgat gcaaaaacca gagccgttcc tgggcaaatg
     2461 aaagtgggaa ctcttgttca cgtgaaaccg gcacgattgg cggcggcgcc gaaagcatcg
     2521 cttcatccgg tttgtttgcc acaagcgctg attggatttc agccgccatc tcttccagta
     2581 ctggaatttc aaacaagcga cgaagtggaa gttcaacctg gaagctgtct cgaatccgcg
     2641 agacaagctg tgccgcaagc agcgaatgcc cgcccaggtc aaagaaattg tcggtcacgc
     2701 tcaaactgtt gaggccgagc agctcggtcc agagttcgac cagggcggct tcagtggcgg
     2761 ttcgtgggcc gatctgttcg ctggctggga gtgcaaattg ttctggttca ggaagttggc
     2821 ggcgatcaat ctttccgtgg gccgtcaatg gaaacatcgt gagcaccaca aatgctgacg
     2881 gcaccatata ttccggaagc accgctttga gtctggcttg aagctcgtga atcagcgcca
     2941 gagtgtcaac ttcatctttc ggtacaacgt aggccaccag tcgctgccca ctgtgcgcat
     3001 catctttgac caggagcagt gattcagcca cttcgggttg ttgattaagt accgcctgga
     3061 tttcgcctgg ctcgatccgg aatccgcgaa tcttgacctg atcgtcaatt cggcccaggt
     3121 attccagctt gccgttgggc agccaccggc caaggtctcc ggttcggtaa aaccgagggc
     3181 taagggctga agacatcggg cttggggcta agggctgagg gctagaaatc aggggctgag
     3241 ggctgagggc cgagggccga ggactaagga tcatcggtga tttataactt tcagccccca
     3301 gccctgagcc ctgagccatg tattgagagc cctgagccct gtcttcagcc ccaagccccc
     3361 agccctcagc cccgagcgcg aagcgctcag ccgttagctc cggtcggttc aaatagccac
     3421 gggcgacacc agcgcctccg acatagagtt cacctttgac tccgatgggt acggggaggc
     3481 cgtggcgatc cagaacaaac acctgcaagt cgggaatcgg tacgccaatc gggctggtct
     3541 ggccacgttc ggcatccgct ttggtcaacg gatggaacgt aacgtgaacc gtcgtttccg
     3601 tgatgccata catattgatc aggcgtggat gcgcgtctcc gtggcgttca aaccagtcga
     3661 gcaaggtgac tggctccagc ttttcacccc cgaaaatcac atatcggagg gcaagttcag
     3721 ccagttcagc ctggtccacc cgcatcagtt gccggaaggc cgatggtgtc tggttcagga
     3781 tggtaacttg ctctcgttgt aggagtttgt agaaatcttc cggcgaacgt gacgtccagt
     3841 aatccaccac cacgagtttg ccgccataga gcagcgctcc ccacaattcc cagactgaaa
     3901 aatcaaatgc aaccgaatga aacagcgtcc agacatcatt ttgatcaaag
//
LOCUS       DAOQDO010000273         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16158_length_3947_cov_8.005139, whole genome shotgun sequence.
ACCESSION   DAOQDO010000273 DAOQDO010000000
VERSION     DAOQDO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16158_length_3947_cov_8.005139"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..2458
                     /gene="ltrA"
                     /locus_tag="PKZ53_05665"
     CDS             623..2458
                     /gene="ltrA"
                     /locus_tag="PKZ53_05665"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNJ39956.1"
                     /translation="MSRNQHVINTAQPMDEWNTTPWKHLEKHVFKLQKRIYRASQRGD
                     VKTVRRLQRLMIHSKSTKTLAVRRVTQDNQGKKTAGVDGKTVLLPAERTSLVLTMKLD
                     GQAKPTRRIWIPKPGSTEKRPLGIPTIDDRAKQAMVKMVLEPEWEAKFEPNSYGFRPG
                     RSVHDAIGAIFNAIRCKPKYVLDADIAKCFDRINHQKLLDKLNTNPSLRHQIKVWLKA
                     GYLDGTELFPTEEGTPQGGVLSPLLMNVALHGMENLLKEYAPSLDMRDKQGHSIGRSS
                     KCKSLTLVRYADDLVVLHENHQVIEECRVQLETWLREIGLEFKESKTRIGHTLNETMG
                     KPGFNFLGFEIRQFKTGKHQSGCNSKGTKLGFSTIIRPSTEAVERHHHEIKAEIRAHK
                     GKSQTELIQKLNPKVRGWCNYYQAVSSKTTFYDLDRLVWQSLWGWAKRRHPGKGKHWI
                     ATKYWKPVGLDRWVFANEGSNPLYLLKHGKTPITRHTKVSGQNSPFDGNLIYWATRLG
                     RHPELPKRVARLFKRQKGKCLQCGLFFKDGDLFEVDHIVPRRLGGLDIRNNLQLLHRH
                     CHDQKTAQDGSQDGMRDKHPIIEERNDGKLSRSVLKPSGRGDPSA"
     gene            2480..2764
                     /locus_tag="PKZ53_05670"
     CDS             2480..2764
                     /locus_tag="PKZ53_05670"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39957.1"
                     /translation="MHPANRVSQSQFHCQRCGHIANADHNGAQVVKKRGIAILKTKSA
                     GASPPSSPAGTRGSARRGSIRRRKESRPAQPAILTDGRQAEARTAQDQPM"
     gene            3045..>3947
                     /locus_tag="PKZ53_05675"
     CDS             3045..>3947
                     /locus_tag="PKZ53_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39958.1"
                     /translation="MEPINVLKNTLHSIPEDLRAEYFQEVLFIYFNRFRIFSVFCLIV
                     FILLLIFFDFLNVFKLPPGPLHTTFTGLHIVAIIVFSGFLLLDQLKKPDSSRDFQPHH
                     KNGLLVVFTFSVVAVHISFLIFLMQRGTPLIYFIGLMFLIVGFPWIDKFPLLPFLLTY
                     LCFFVSNFFYLKAESVIEINTMGFVATSLAYIFARTVTELRVQNFLDHQTIQRQTQEL
                     NQKVVELSAAHRDVAEKNVKLDHQIQEVKRMNQELVASQQQANRIFSALAEALPGTIL
                     DGKYRLDEKIGAGGFGVVFRGTHLL"
BASE COUNT         1123 a          976 c          978 g          870 t
ORIGIN      
        1 cggcgtcgaa aactggtgat cagcgtttca cctcaccaat catcgcaaga atgtgtgcga
       61 tccgtttacc cgaaatggga ttttcccagg gatgggtaac aaaacatccg accaggatga
      121 tttgataact aaatagccat aagggtgaaa atcccttcct ccaggcgttg gagtgacagc
      181 accgccctcc ggtagcgact cgccatcaat ccggaaagcg ggcgggaacg gggttcagaa
      241 agatagttcc agtcattctt ccccaagcaa acaatttatg gacagcgaaa gcgaagatat
      301 gatgactcgt cgtgagcgag aaccagccaa aggaactgac aggctgggcc aaaaggcaaa
      361 ggataagcgg gttggcaatg acattcttga ccaacaaacg ccccacggaa acccggcgaa
      421 cagtatcgtc ctaaagattg acagcatggc tatttggaac ggagtaaccg cttgcgttct
      481 ctcaggcaaa agcctgagca gtcagcgaag acgcaacctc tgaaattcag gaacggaagc
      541 ggagggattg tatcaaaagc caacgccctg agtaatgtca gggatatgca gacagataca
      601 caatgattgg aaaggtaagg ccatgagtcg caatcaacac gtcataaaca cggcccagcc
      661 gatggatgaa tggaacacaa ctccgtggaa acatctggag aaacatgtgt tcaagttgca
      721 aaaacggatt taccgagcca gccagcgtgg agatgttaaa acagttcgac gactgcaacg
      781 attgatgatc cattccaaat caaccaaaac cctggccgtc cgccgggtga cacaagacaa
      841 ccagggaaag aaaacggctg gggtggacgg aaaaaccgtc ctcctcccag cggaacgaac
      901 ctctctggtg ttgacgatga aactagatgg acaagccaaa ccgacgagaa ggatttggat
      961 acccaagccc ggatcaacgg aaaaaaggcc gttagggata ccgaccatcg acgaccgtgc
     1021 caaacaggca atggtcaaaa tggtgttgga accggaatgg gaggccaaat ttgaacccaa
     1081 ctcctatgga ttccgacccg gacgatcagt ccatgatgcg attggagcca tttttaatgc
     1141 cattcggtgc aagccgaaat atgtgcttga cgctgatatt gccaaatgct ttgaccgcat
     1201 caatcaccaa aagctcttgg ataagctcaa caccaatcca tccttgagac accagatcaa
     1261 ggtctggctc aaggcggggt atctggacgg aaccgaactt tttccaaccg aggaaggaac
     1321 tccccaaggt ggagtgctgt caccattact gatgaatgtg gcactccacg gaatggaaaa
     1381 ccttctgaaa gaatatgccc catccctgga tatgcgtgat aaacaaggac actcaatcgg
     1441 gcgaagcagc aaatgcaaaa gccttaccct tgtcaggtac gcggatgacc tcgtcgtttt
     1501 gcacgaaaat catcaggtga ttgaagaatg ccgggtgcaa ttggaaacgt ggctgagaga
     1561 aattgggctg gaatttaagg agtcaaaaac caggattggt cataccctca atgagacgat
     1621 gggaaaacca ggatttaact ttctgggatt cgagatccgt caattcaaaa ctgggaaaca
     1681 tcaatctgga tgtaattcca agggaactaa gcttgggttc agcaccatca tccgaccttc
     1741 caccgaggcg gtagagcggc atcaccacga aatcaaagcg gaaatccgtg cgcataaagg
     1801 aaagagtcaa acggaactca tccagaaact caatccgaaa gtgcgcggat ggtgcaacta
     1861 ctaccaggct gtgagcagca aaaccacgtt ttacgacttg gaccgactgg tctggcaaag
     1921 cctttgggga tgggccaaac gtcgtcaccc aggaaaagga aaacactgga ttgccacgaa
     1981 atattggaaa ccagtgggac ttgaccgttg ggtttttgcc aatgaaggaa gcaacccgct
     2041 gtacctgctc aaacacggga aaacaccaat tacccgtcac acaaaggtca gtggacagaa
     2101 ctcacccttt gatggcaacc tgatatactg ggccacaaga ttaggccgac accctgaact
     2161 gcccaagcga gtcgcaaggc tcttcaaaag gcaaaaagga aagtgccttc aatgtggact
     2221 gtttttcaaa gatggggatt tatttgaagt cgatcatatc gtcccaagaa gattgggagg
     2281 attggacatc agaaacaatc tccagctttt acaccgacat tgccacgatc aaaagactgc
     2341 gcaagatggc agtcaggatg gtatgcgtga caaacatcca attattgagg agcggaatga
     2401 cgggaaactg tcacgttccg ttttgaagcc gagtggaagg ggtgaccctt ccgcttaggg
     2461 taacgcccgg tgcggccaca ttcatcccgc caaccgcgtg agtcaaagtc agttccattg
     2521 tcagcggtgc ggacacattg cgaatgccga ccacaacggc gcccaggtgg tgaagaaacg
     2581 tgggattgcc atcctgaaaa ccaaatcggc gggtgcgagt ccgccgtcat cacccgccgg
     2641 gacgcggggg agtgcgcgtc gagggagtat cagacgccgg aaggaaagcc gaccggcgca
     2701 gcccgcaatc ctgactgatg gccgtcaggc agaagcgcga actgctcaag atcagccaat
     2761 gtgagttggc tgatcccacc cagaagctcg gccatttatg gccgagaggt tcactaagat
     2821 gtttgacctt gccgcgtggg gatgcccgga aatgtagcac aggaaaaacg aaacggaacc
     2881 cccatctcat tggaccagca aaagggggag tttacccaaa tcaggaattg cgctatcttg
     2941 tgcaggctgc ttctcaatat cactcacaac caaggccaaa cctcttcaat ccagtaacct
     3001 gtttcctgaa ttcttttggt tactttatcg atttattcga tcatatggaa ccaatcaatg
     3061 tactcaaaaa cacacttcac tctattcctg aagacttgcg tgccgagtac tttcaggaag
     3121 tgctgtttat ttacttcaat cggtttcgaa ttttttcagt attctgtctg attgttttta
     3181 ttctactcct gatctttttt gattttctca acgtcttcaa actgcctccc ggaccgctgc
     3241 acaccacatt taccggcctg catatcgttg ccattatcgt attttcaggg tttttactgc
     3301 tggaccagtt gaaaaaacca gattcgtcac gtgattttca accacaccac aaaaacgggc
     3361 ttctggtggt atttaccttc tcagttgtgg cggttcatat ttcatttctc atttttttaa
     3421 tgcagcgcgg aactccactc atttatttta ttgggttgat gtttctcatt gttggctttc
     3481 cctggattga taaatttcca ttgctcccgt ttctgctgac ctatctttgc ttttttgtat
     3541 ccaatttctt ttatctcaaa gccgaatcgg tcatcgaaat aaacacaatg gggtttgtgg
     3601 ccaccagtct ggcctatatt tttgcccgga ccgtgacaga gcttcgggta cagaacttcc
     3661 tcgaccatca aaccattcag cgccaaaccc aggaactcaa ccagaaggtc gtggaactca
     3721 gtgcggctca ccgggatgtg gccgagaaaa atgtcaaact ggaccaccag atccaggaag
     3781 ttaagcggat gaatcaagaa ctggtggcct cgcaacaaca agccaaccgg attttctcag
     3841 ctctggccga agcgctgccg ggaacaatcc tggacgggaa atatcggctc gatgaaaaaa
     3901 tcggcgccgg tgggtttggc gttgtgtttc gcggcacaca cctgctg
//
LOCUS       DAOQDO010000274         3930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16291_length_3930_cov_2.482581, whole genome shotgun sequence.
ACCESSION   DAOQDO010000274 DAOQDO010000000
VERSION     DAOQDO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16291_length_3930_cov_2.482581"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKZ53_05680"
     CDS             <1..651
                     /locus_tag="PKZ53_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39959.1"
                     /translation="DQALRQITIELDEAGLKLARENGLVQKVILPVKIPGAYQLRVGI
                     RDAGSAHIGSVNQFVEVPNLKKGALALSGIMAAGMKPAAGNTPPASGGQQTQADPEWS
                     APEASPAVRRIQRGNVVHYGYDIFNPQVDSQTHQPNLEVQVTLFRDGQLVFQGQPRPL
                     ALSPQNDWKQLRTGGQLQLGTQMDPGQYVLQLTVTDKVRKRGATSVSQWVDFEVLE"
     gene            1053..1550
                     /locus_tag="PKZ53_05685"
     CDS             1053..1550
                     /locus_tag="PKZ53_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39960.1"
                     /translation="METAETDAYRERVATTLVVWVGLGLLIYSQFAYTIDHSFLSPTG
                     HWRAKLVDYKQADHKIEIKLEDTTHHQPAKMIYAVDELPSFANERILWSKDETKLLVV
                     GRGCSVPNDCSKLATGEGLYLLYDIPTGTTRCNIRHSTTAFNYADVAQYEFVPPLPKD
                     GKLAK"
     gene            complement(1552..2511)
                     /locus_tag="PKZ53_05690"
     CDS             complement(1552..2511)
                     /locus_tag="PKZ53_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNJ39961.1"
                     /translation="MKIVIPGGSGQVGTILARAFQEARHEVVLLSRTRQPSPWRVITW
                     DAESLGLWASELEGADVVINLVGRSVNCRYTAENRRQIIDSRIKSTQVIGQAIAQCKN
                     PPRVWLQASTATIYAHRYDAPNDEATGIIGGTEPGVPETWRFSIDVATGWEKAAEAAV
                     VPNTRKVLLRSAMTMSPDRGGVFDVLLGLTRVGLGGQSGNGRQYVSWIHDLDFIRAIN
                     WIIEHDHLSGPVNLSSPYPVPNAEFMRILRKAWGIPVGLPATDWMLEIGAVFLRTETE
                     LILKSRRVTPGRLQESGFSFVFPTWEAAAADLCRRWRFSQHGR"
     gene            complement(2626..3792)
                     /locus_tag="PKZ53_05695"
     CDS             complement(2626..3792)
                     /locus_tag="PKZ53_05695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNJ39962.1"
                     /translation="MKYSGSTGLKFVFLILITIFPVSVPAQNTSDALTQELNRLYAGS
                     KFPGFCVAIVDANGIAYQQGFGLADVEKKVPYSPKTIQPVGSVSKTLIGVALLKAIEQ
                     GHFSLKTNVNDLLPFQVSNPHFPGKVITIEHLATHTSSIVDREAVYKKVYVPGKKADS
                     TIQEFLTAYLTSNGKLYQKENFARNEPGTAYNYSNIGATLAAYLIELKTGMSFASYTQ
                     RFILKPLGMDSSGWVYDDQQADNATLYEASRKVVPTYSLVTYPEGGLRTSGRDLSQYL
                     IEIIKGYAGKSSLLTRESFQTMLSPKFAAAGLPKNIDPKEPNQGIFWQFRRNGTIGHS
                     GGDPGVTAFLSFNPKTGKGKIFLTNILIEENDLAGQFSAIWKTLESYEDKIDGQ"
BASE COUNT          964 a         1032 c          923 g         1011 t
ORIGIN      
        1 gatcaggcac tgcgccaaat cacgattgaa ctcgacgaag ccggactcaa actggccagg
       61 gaaaacggcc tggtccaaaa ggtaattttg ccggtgaaaa tacctggcgc gtatcaactt
      121 cgggtgggta tccgtgatgc aggttcagca cacattggat cagtgaacca gtttgttgaa
      181 gtccccaacc tgaaaaaggg agcccttgcc ctgtcgggaa tcatggccgc tggaatgaaa
      241 cctgccgctg gaaacacacc tcctgcctca ggtggccaac agactcaggc cgatcctgaa
      301 tggagtgcac cggaagcgag tcccgcagtt cggcgaatcc agcgcgggaa tgtggtgcac
      361 tatggatatg acatttttaa tccgcaggtt gattcacaaa ctcatcagcc aaatcttgaa
      421 gttcaagtca cgctcttccg cgatggccag cttgtctttc aaggtcagcc ccgaccgctg
      481 gcactttctc cccaaaatga ctggaagcaa ctccggactg gaggacaact ccaacttggg
      541 acccaaatgg atccaggtca gtatgtgctc caactcacgg taaccgacaa agtacggaag
      601 aggggagcga cgtcggtcag tcagtgggtt gattttgaag tacttgagta atatcagtca
      661 gtggccagta gctcactcaa tctatttgat tgaataactt gactctttcc tgactcagag
      721 ttctcattgc aagctgggaa cctgatcttt cagaagaaca actcggaatg agagtgcgga
      781 ttgctccatt caattcgcat ttttcgtttc gatagaatca gtctcatcac gcttcagggc
      841 ttcaaatttt tccattgagg aaccgttttt tttccagtac gatggcggct ccttttcaaa
      901 ctcccaacac cttttcgatg atttaaactg gtactgacaa ccctggtttg agagcaaggc
      961 tggttggata ttttgatatc caaacacgcc tcatctgctc cagcttaccc gcatcgtaag
     1021 tcctttttct tttgcccctt tggaggaaag tgatggagac cgctgaaact gacgcctatc
     1081 gtgagcgtgt tgccaccaca ctggtggtgt gggttggttt gggtctattg atctacagcc
     1141 agtttgccta caccattgat cattcattcc tgtcgccaac tggccactgg cgcgccaaac
     1201 tggttgatta caaacaggct gatcacaaaa ttgagattaa acttgaagac acaactcatc
     1261 accagccggc caaaatgatt tatgcggttg acgaactccc ctcttttgcc aacgagcgga
     1321 ttttgtggtc caaagatgaa accaaactgc tggtcgttgg gcgtggatgt tcagttccga
     1381 atgattgctc aaagctcgcc acgggtgagg gcctgtattt gctgtatgac attccgaccg
     1441 gaaccacacg gtgtaatatt cgccactcca ccaccgcatt taactatgcc gatgttgccc
     1501 aatatgagtt tgtcccaccg ttgccaaaag atggaaaact ggccaaatga cttaccgccc
     1561 gtgctgagag aatcgccagc ggcggcaaag atcggcggcg gcggcttccc aggttggaaa
     1621 tacaaaggaa aagcctgatt cctggagcct gcccggtgtc acccggcgac ttttgaggat
     1681 cagttcagtt tctgttcgca gaaaaacagc cccgatttcc agcatccagt ccgtcgccgg
     1741 gagcccaacc gggatgcccc aggcttttcg caaaattcgc ataaattctg cattcggaac
     1801 tggatatggt gatgaaagat tgactggccc tgacagatgg tcgtgctcga taatccagtt
     1861 gatcgcccgg ataaaatcca ggtcgtgaat ccaggaaaca tattgacgcc catttcctga
     1921 ctgtccaccc aacccaaccc tcgtcagtcc aagtaacaca tcaaagacgc ctccccggtc
     1981 cgggctcatc gtcatcgctg accggagcag cacttttcga gtgtttggaa caactgccgc
     2041 ttccgctgcc ttctcccagc cggttgccac atcaatgctg aaccgccacg tttccggcac
     2101 gccgggttca gttcccccaa tgattccagt tgcctcatca tttggagcat cataccgatg
     2161 ggcataaatc gtggcggtgc tcgcctgtaa ccacacacga gggggatttt tgcattgggc
     2221 aatggcttgc ccaatcacct gtgttgattt gatcctggaa tcaatgattt gccgtcggtt
     2281 ttcagccgtg tagcgacaat tgaccgacct tcccaccaga ttgatgacga catcggcacc
     2341 ctccagttcc gaagcccaca accccaaact ttcggcatcc caggtaatga cacgccacgg
     2401 cgaaggctgc cgggttcgac tcaaaagaac aacttcgtgt ctagcctcct ggaaggctct
     2461 ggccagaatc gttccaacct gtccgcttcc gcctggaatg acaattttca tatgttgatg
     2521 atcctaaacc agttcgttgg aaagttgtcc agctactatc tgtctacatc attcgtagta
     2581 aattgagttc attgaggttc catccacaat tttcagagtt gaccatcact gaccatctat
     2641 cttgtcttca taactttcca gcgtcttcca aatggctgaa aactggccag ctaagtcgtt
     2701 ttcttcgatc agaatgtttg tcaggaaaat tttgcctttt ccagtttttg gattaaaact
     2761 gagaaacgcg gttactcctg gatcaccgcc cgagtgccca atggttccgt tgcgtctgaa
     2821 ctgccaaaaa attccctggt ttggctcctt cgggtcaata tttttgggca aaccggcggc
     2881 ggcaaatttt gggcttaaca tcgtctgaaa agattccctg gtcaaaagcg acgatttccc
     2941 cgcatagcct ttgatgattt caatcagata ctgactcaga tcacgccccg aagtccttaa
     3001 cccaccttcc ggataagtaa ccagcgaata ggtcggaaca acttttcggc tggcttcata
     3061 caacgtggcg ttgtccgctt gctggtcatc atacacccaa cctgaactgt ccattcccaa
     3121 aggcttgaga atgaagcgtt gggtgtagct ggcaaatgac atgccagttt tgagttcgat
     3181 cagatacgcc gccagcgttg ctccaatatt cgagtagtta tatgccgtac cgggttcatt
     3241 tcgggcgaag ttctctttct gatataactt cccgttgctg gtcaggtacg cagtcagaaa
     3301 ctcctgaatg gtggaatcag cttttttccc tggcacatac acttttttgt agactgcttc
     3361 ccggtcaaca atgctggaag tatgagttgc aaggtgttca atggtaatga cctttccagg
     3421 aaaatgcggg ttcgatacct ggaaggggag caggtcattg acgttggtct tcaagctaaa
     3481 atgtccctgt tcaatagctt tcaagagtgc cacaccaatc aaggttttgc tgaccgatcc
     3541 aaccggttga atagttttgg ggctgtaggg aaccttcttc tcgacatcgg caagcccaaa
     3601 gccttgctgg taggcaatgc cattggcgtc aacaatggcc acgcaaaacc cagggaactt
     3661 tgaaccagca tacagccgat tcaattcctg agtgagcgca tccgaagtgt tttgagccgg
     3721 cacactcacc ggaaaaatag ttatcagaat caaaaacacg aacttcaaac ccgtgctgcc
     3781 agaatatttc attgcgtttt atccctgcct ttttgcgaga tgatgagaga tgtcttctta
     3841 ttgtgccctg aagcccattt cgttgcacga gcaaagattg gtaaaccact ggtttctgca
     3901 tacctctttc cacatcacag attgggaggg
//
LOCUS       DAOQDO010000275         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16301_length_3928_cov_3.350891, whole genome shotgun sequence.
ACCESSION   DAOQDO010000275 DAOQDO010000000
VERSION     DAOQDO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_3928_cov_3.350891"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKZ53_05700"
     CDS             complement(<1..445)
                     /locus_tag="PKZ53_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39963.1"
                     /translation="MSTPIPMPTQAIQPEEIENLDGSTTLYTYFDPTNETMLALKSDL
                     FEKHWRQIVVGPCIEGSVFEVRFTEAPKVTVLDGYLTVDLGPWHFHLCITDHKGTRPA
                     ELAQKRRVAKVAFFENKGHKCGGGVSWGLRMWNGFGEQMTTVFLPN"
     gene            complement(610..2973)
                     /locus_tag="PKZ53_05705"
     CDS             complement(610..2973)
                     /locus_tag="PKZ53_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ39964.1"
                     /translation="MFFSKLISAVIFTVVAVTSVWAQSREAGTLRGKIVDASSAAIAG
                     ASIRLFSRDNRIQVAGKSDQSGSYVFAQLAPGEYLVVVEAEGFVSTAGTGIRIDPTGI
                     HNLDFSLDVAGVQSDIVVTAAGVPQTVTEQSRALTVVDQIEIDQRNETFVPEILRATP
                     GVRVQQLAGPGSFTTVRFRGLRDADTSILIDGLRLRDASGFRGDFSGFLEDLMANNLD
                     RVEVVRGSSAPLYGTAATGGVVNLVSRVGSGAPHGSVSFEGGTLGQLRERFTLAGGVR
                     ERFGYSFGVTRTDVLDGIDGTDVFRNTSFGTHLVYNFTPNLTLSGHMSASVSPRLDLN
                     ASPFPTGPAGNELGYETGTGPVTGFVFDLNDPDSRRTSSLLAGWTSLRHRVSDFWSYT
                     VSYQGTRTRRNFSNGTGQDPFLTQLGVFDFPFETSRLDGENQVIESRHTLQFSRNHFL
                     TVGVEHEREAFTQEFVSETFGIRNAPTTDRQNSTAFFFNDQLSFLNRNLQIGFGFRGQ
                     WFSVANPESVPELNGLSAPAAYTGDGSISYYFEKSQTKIRAHIGNSFRAPSLSERFSF
                     FNSSQGRQRLGNPLIRPERALSVDGGLDQTLFGGRLRVSATYFYTRLQELITSTTLFQ
                     QVNVRGGLARGAEVEVAATPVRGLDLRFGYTYSDSQFSPNFATLRADNTTIPAGIARQ
                     QFGIPKHQVSFGANYAISRWNFNFDFSGISQYDEPLFSPNVFKQVVFTFDGYKKADVG
                     VSYTLPLTDGTSLRFYTKVENVLNQRYFEDGFRTPRVVALGGCAFRF"
     gene            3452..>3928
                     /locus_tag="PKZ53_05710"
     CDS             3452..>3928
                     /locus_tag="PKZ53_05710"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNJ39965.1"
                     /translation="MPKKILCGLLALFLCCFLTRAISAQTDARSVTGKVTDQSGGAIV
                     TALVTLLDSQTGKARTTTTDVNGEFNFGNVVDGTYRLSAEKPGFVSLTKDVVPGEPLT
                     SLVLQVSGVSDVVEISAESYLVTDATTAAKINAPLRDIPQSVQVINQNLLRDRATFN"
BASE COUNT          965 a         1047 c         1029 g          887 t
ORIGIN      
        1 ggttggggag aaagaccgtc gtcatttgct cgccaaaccc attccacatc cgcaagcccc
       61 aactgacacc accgccacac ttgtggcctt tgttttcaaa aaaggcgact ttggccaccc
      121 ggcgtttttg ggccagttca gccggacgtg tgcctttgtg gtcggtaata cacaggtgaa
      181 agtgccaggg gccgagatca accgttaaat atccgtccag caccgttact ttgggggctt
      241 cggtaaaacg aacttcaaaa actgaccctt cgatacaggg gccgacgaca atctggcgcc
      301 agtgcttttc aaacaagtcg gatttgagcg ccagcatggt ttcgttggtt gggtcaaaat
      361 aggtgtacag cgtggtactg ccatccaggt tttcaatttc ttccggttga atggcctggg
      421 ttggcattgg aattggcgtg gacatagagt gaaagttcct ttgtgagaaa agatggttcc
      481 aattggaaaa aaggcggcaa tttttggggt ttcagatgcg cagaagcaag aatgttcgca
      541 tgcgaacatt ccctgtaata atttgatgat caagcgttta acctgagaaa gtatttctct
      601 ggaatcggtt tagaaccgaa atgcgcatcc gccgagtgca accacacgcg gcgtgcggaa
      661 cccgtcttca aagtaacgct ggttcaacac attttcgact ttggtgtaaa agcgcaggct
      721 ggtgccgtcg gtcagcggca acgtataact gacaccgaca tcagcttttt tgtagccgtc
      781 aaaagtaaaa accacctgtt tgaaaacatt tggtgaaaag agcggttcgt catactggct
      841 gattccagaa aaatcaaaat tgaaattcca gcgcgaaatc gcatagttgg cgccgaaact
      901 gacctgatgt ttcggaatcc caaactgctg acgggcaatt ccggctggaa ttgtcgtgtt
      961 gtctgcccgg agtgtggcga agttggggga aaactgtgaa tcggaatagg tgtacccaaa
     1021 tcgaaggtcc agcccgcgaa ccggggttgc ggccacttca acctcggcgc cgcgtgccag
     1081 accaccgcgc acgttgactt gttggaacaa cgtggtcgag gtaatgagtt cctgtaatcg
     1141 agtataaaag taggtcgcgc taactcgcag acgtccgcca aacaaagtct ggtccaggcc
     1201 gccatccacc gacaacgctc gctctggacg aatcaaggga tttcccagcc gctgccgtcc
     1261 ctgacttgaa ttgaagaaag aaaaccgctc agacagcgat ggcgcccgaa aactgttgcc
     1321 aatgtgggcc cggattttcg tctgggattt ttcaaagtaa tagctgatgg acccgtcgcc
     1381 agtgtaggcc gccggtgccg aaagtccatt gagttccgga acgctttctg gattggcaac
     1441 ggaaaaccac tggccccgaa acccaaaccc aatctggagg ttgcggttaa gaaacgaaag
     1501 ctggtcgttg aagaaaaatg cggttgaatt ttgccggtca gtggttgggg cattgcgaat
     1561 gccaaaggtt tcagagacaa attcctgggt aaaggcttcg cgctcgtgct caactccgac
     1621 cgtcagaaag tggttgcggc tgaactgcag cgtatggcga gactcgatga cctggttttc
     1681 gccgtcaagc cgcgacgttt caaacggaaa atcaaacacg ccgagttgtg tcaggaacgg
     1741 atcctgacct gttccattgc tgaaattgcg gcgggtccga gttccctggt atgaaaccgt
     1801 gtagctccaa aagtcgctca cccgatgtcg gagcgaggtc cacccagcaa gcaggctcga
     1861 cgtgcgacgc gaatccgggt cattgaggtc aaaaacaaac cccgtcaccg gtccggtgcc
     1921 ggtttcatat ccaagttcat ttccagccgg acccgttgga aacgggctgg catttaaatc
     1981 aaggcgtggt gacaccgaag ctgacatgtg cccgctgagc gtcagattgg gcgtgaaatt
     2041 gtaaaccaga tgcgttccaa atgatgtgtt gcgaaacaca tccgtaccat caatgccgtc
     2101 aagcacatcg gtgcgggtaa ctccaaagct gtagccaaac cgctctcgta ctccgccagc
     2161 cagagtaaat cgttcccgca actggccaag ggttccgcct tcaaagctga cgctaccgtg
     2221 tggcgcgccg cttccaaccc gtgacacaag gttgaccaca ccacccgttg ccgcagtgcc
     2281 atagagcggc gccgatgaac cgcgcaccac ttccacccgg tccaggttgt tggccatcag
     2341 gtcttcgaga aaaccgctga aatctccgcg aaatcctgac gcatcccgca gccgcaagcc
     2401 gtcaatcagg atcgaagtgt cggcatcgcg caacccacga aaccgaaccg tggtaaacga
     2461 gccggggccg gccagttgct ggacccgcac gcccggtgtg gcccgcagga tttccggtac
     2521 aaacgtttcg tttcgctgat caatctcgat ctggtcaacc acggtcaacg cccgggattg
     2581 ttcggtcacc gtttggggga cgccggctgc cgtgacgaca atgtctgact gcacacccgc
     2641 cacatccagc gagaaatcca ggttgtggat cccagtgggg tcaatccgaa tgcctgtgcc
     2701 tgcggtggaa acaaatcctt cggcttcaac caccaccaga tactcacccg gtgccaattg
     2761 ggcaaacacg taacttccgc tttgatcgct tttcccggct acctgaatcc ggttatctcg
     2821 tgaaaacaac ctgatggacg ccccggcaat tgcggctgaa ctggcatcca caattttccc
     2881 acgcaatgtc ccagcctctc tggactgtgc ccaaacagag gtaacagcca ccaccgtaaa
     2941 aatcacagca gagataagtt ttgaaaaaaa cacgcggtcc tcgctctcac aaacagtaca
     3001 aaaatttggc cgatacccaa ttccagggcg gaatcctttt gaatcgtgcc tcaggcccgc
     3061 catcaatcgg taacaaccag ggcgcaatgc tggacctggc acctgggtta cagcactgat
     3121 ttttcaatga atggttgttt cagccagtgt gagtcgaaga aagtggaagt cgaactgaaa
     3181 gttgtgggtt gaaactctgg cgtgcggtga cctttcaccg ctttggcata ccggcaactg
     3241 attgaggagg aaccgaattc gtacaagagg cagtcgctct cagaaaacag gtatctcaga
     3301 agttattggg tgaactctgg ttttataaaa ttttttatag ttttacagta aaatttcttg
     3361 acattgataa ttttttccgg ttatacaggc acttcttctt ctcaccctgg cccccttgcg
     3421 gtgaatctga ctcatatcaa actggacctg tatgccaaaa aaaattctct gtgggctgct
     3481 tgccctgttt ctgtgttgct tcctgaccag agccatatcg gctcaaaccg acgctcgatc
     3541 tgtgaccgga aaagtcactg accagagtgg cggcgccatt gtcacggcgc tggtgacctt
     3601 gttggattcc caaaccggaa aagcgcgaac caccaccacg gatgtcaatg gggagttcaa
     3661 ctttggcaac gttgtggatg gaacctaccg gctgtcggcg gagaaaccgg gatttgtttc
     3721 tctgacaaag gacgttgtcc ctggcgaacc cctcaccagt ctcgtgttac aggtttcagg
     3781 cgtcagcgat gtggttgaga tctctgctga gagttatctg gttaccgatg cgactacggc
     3841 ggccaaaatc aacgctccct tgcgcgacat tccgcaatca gtccaggtca tcaaccagaa
     3901 tttgttacgt gaccgggcga cattcaac
//
LOCUS       DAOQDO010000276         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16367_length_3920_cov_3.153169, whole genome shotgun sequence.
ACCESSION   DAOQDO010000276 DAOQDO010000000
VERSION     DAOQDO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16367_length_3920_cov_3.153169"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..1277)
                     /locus_tag="PKZ53_05715"
     CDS             complement(339..1277)
                     /locus_tag="PKZ53_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNJ39966.1"
                     /translation="MTRAYSTAEVSQMWNVSESTIKRWADSGKLECIRTPGGHRRFRM
                     EALMAFQEQNHLKSSNLFTTPLSISSLSDSALNQNHHVGQEQHLLEQVETVTEFTPVK
                     TLQQLYFDMAIQGSQKLAEAFLQRLYLHGYSILDIKEKVLTPVMHQIGWLWQRGELQV
                     YEEHLASHVTLSATRYLHYLVPHKSEHGKVALCACLEGDFHEIALNWLTQTLESDGWR
                     VIVAGANTPVHSISDALNRFQPTILCLSATILSNLEHLRRDYKTCWERARELGTRVII
                     GGAAFDDPDTRAHFPHHFHGTSFSDLIGYLRREFRH"
     gene            1852..2853
                     /locus_tag="PKZ53_05720"
     CDS             1852..2853
                     /locus_tag="PKZ53_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNJ39967.1"
                     /translation="MRELLEEPGTIALMARAETVARMIDEEILKDIAEVTQPLLLEAT
                     TYLFKSGGKRLRPFLVITCAEALGGVLDDAIPPALAVEYLHNLSLIHDDMMDHSEKRH
                     GRPTLHSQFGDRTSLLVGDLLYAKAVEQASRVARHAIRMVDVLAKAAKLMCLGQFDDM
                     SFETRMELEIEDYLVMASRKTSALYRASCICGVLAGDGTEEQVKAMALFGEKIGTAFQ
                     IWDDVLDLQADPLRLGKPLGLDIRDGKKTLIVIHFLKYAKSPERERFLQYLGNPNLSR
                     ELPEAVALLEAAGSIAFAREIAWNLLEDAKQQLHVLPPSPARDLLYQYADFLLQRNY"
     gene            3011..>3920
                     /locus_tag="PKZ53_05725"
     CDS             3011..>3920
                     /locus_tag="PKZ53_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNJ39968.1"
                     /translation="MGRDFIDRRQLNWKRLEEMLSLALRGQLSHLHREQVREFGRLYQ
                     RTAADFAIAHQEIRDSHLVNYLNNLVGRAHGTIYRSKSSGFKEIWEFYRYSAPALFRE
                     TWRYTLAAFLVFMVVGVFSGVFVFLDDQYSLRLAPEVLDMVKSKQNWTEMINKNNQAA
                     SSMIFTNNARVAVYSFATGIFLGLGSLYFMATNGLHIGGVTAMCIKYKFTPILIFMAA
                     HGVFELTAIFIAGGAGFLIGSALLMPGNLRRGEALIERGIKAIKLMMICLPLLVVAGL
                     IEGFLSPLNISPAYKLAVSGATALFLV"
BASE COUNT          927 a          912 c         1038 g         1043 t
ORIGIN      
        1 aagggtaatt ttgcgcctgt gctgcatcca ccaggcccac cacgcttacc aaaagaagtg
       61 tcattagtga agtcgtcagt ttgttcataa tcgggctcct catccagaag ttcctgtgta
      121 tctatcaata ttgtttcagc atcgctgctg tatgtttgcg ccgatttgac acaccaggaa
      181 tttgaatcgt tgcctgattt ttttataaat tattgtgaat aaagaggttg tgcacgaaaa
      241 ggtgtgaaag gggacagact ggtgaccaaa tggtgtagtg gtgagatctg gtcaggaaga
      301 tggtgacctg gccagaaccc tgtctgccgg acctcaaatc agtggcgaaa ctcccgccga
      361 aggtacccga tcaggtcgga aaaactggtg ccatgaaagt gatggggaaa gtgagcgcgg
      421 gtatcagggt cgtcaaaggc ggcgccgcca ataatgaccc gagttcccag ctccctggcc
      481 cgttcccagc aggtcttata atctcgccgt aaatgctcca gattggacag gatcgtcgcc
      541 gataaacaca aaatcgtcgg ttgaaaccgg ttgagggcat ccgaaatcga atgaaccggg
      601 gtgttggccc cggccacaat cacccgccag ccatcagatt caagggtttg agtcaaccag
      661 ttgagcgcaa tttcatgaaa atccccttcc aaacaggcgc acaatgccac cttgccatgc
      721 tcggatttat gtggcaccag gtagtgtaaa tacctggttg ctgaaagggt tacgtgtgaa
      781 gccaggtgtt cttcataaac ctgcaattca cctcgttgcc ataaccaccc aatctggtgc
      841 atgacagggg taagcacttt ttctttgata tccagaattg aatatccgtg caggtataac
      901 cgctgcagaa atgcctcagc cagtttctga ctcccctgga ttgccatatc aaaatagagt
      961 tgctggagtg tcttgaccgg agtgaattcg gtcaccgttt caacctgctc aagcaggtgc
     1021 tgttcctggc caacgtggtg gttctggttc agggctgaat cagaaagtga tgatatagaa
     1081 aggggagtgg tgaaaaggtt tgatgacttc aggtgatttt gttcctgaaa ggccattaac
     1141 gcttccatgc gaaaccgacg atgcccccca ggtgtccgaa tacactccag tttcccacta
     1201 tcagcccacc gtttaatggt ggattcactg acattccaca tctgagaaac ttccgcagtt
     1261 gagtaagcgc gagtcataga tcaccatctt caggatttca gtaacaacga atcgcttgct
     1321 ccagttcaag atcactcaaa aggaagcaat ggtcgttttg gtcgttttta ttggtcgagt
     1381 taaacctacc agattccatg tagaggaaca aggtgtttta tcggagaata agttgcctta
     1441 aggtgaatta ttgtttttat tggttttatt gtgattttgt cggaatttgt aaggaagtta
     1501 ggatgatttt agggaaaagg gtattgaccg atttggtcgt ttttcgtata gtcagttcat
     1561 tcaatgagcg atcttattgg acactgtctg tagggcgttc attgtcgatt tggcacatcc
     1621 tacttctgag ttgtaggctt ggagggatcc ggggggaaac ttcaggtctg ctgccaacgc
     1681 ttgggggcct gtaggatgtg ccaaaactat tttcggctcc tggtgactga gcaaactacc
     1741 ctgtgggctg gcatgccttt gacaaatgag ttgccgaaag cttgtgcttg cgagtgcatc
     1801 tggcccattg taggatggtg agaattacat caccgagaaa agcgggggac catgcgcgaa
     1861 cttcttgaag agccgggaac gattgccctg atggccaggg cagaaaccgt agcccgaatg
     1921 attgatgaag agattttgaa agatattgct gaggtaacac agccgcttct attggaagca
     1981 accacctacc tgttcaaaag tggcgggaaa cgattacggc ctttcctggt tatcacctgc
     2041 gctgaagcac tgggtggtgt tctggatgat gccattcctc cagccctggc cgttgaatac
     2101 ctgcacaatt tgtcattgat ccacgatgat atgatggatc acagcgagaa acggcatgga
     2161 cggccaacgc tccatagtca gtttggtgat cgaacctcgc ttctggtcgg ggacctgctc
     2221 tatgccaaag ctgtcgagca ggcgtctcgc gttgcccgcc atgccattcg catggttgat
     2281 gtactggcca aagctgccaa attgatgtgt ttaggtcaat ttgacgatat gagctttgag
     2341 acccggatgg agcttgaaat cgaagactat ctggttatgg cctcgcgcaa gacgtcagcc
     2401 ctctaccggg caagctgcat ttgcggtgtt ctggccgggg atgggaccga agaacaggtc
     2461 aaagccatgg ccctgtttgg ggaaaagatc ggtaccgctt tccagatttg ggatgatgtg
     2521 ttggatcttc aggccgaccc cctccggttg ggcaagccac ttggccttga tatccgcgac
     2581 gggaaaaaga ccttgattgt gattcacttc ttgaaatatg ccaaatcacc cgaacgcgag
     2641 cggttcctcc agtatcttgg caatccaaac ttgagtcgtg aattacctga agccgtggcg
     2701 cttctggaag ccgctggttc gattgcgttt gcccgcgaaa ttgcctggaa tttgcttgag
     2761 gatgcaaaac agcagcttca tgtgctgcct ccatcaccag cccgtgacct gctctatcag
     2821 tacgccgatt ttctgctcca gcgaaattac tgatccaggg cggaagaaca cgggcggaag
     2881 acatcgggca gaagaaaatg ggcttggggc tgaagattcg caagctcagg gcggaagaag
     2941 acaggctaaa gaagtttgat ttcattcttc attcttcgtt ctcaattctc aattcttcat
     3001 tcttctcttt atgggccgag attttattga tcgccgacaa ttaaactgga agcgactgga
     3061 agagatgctg tctctggccc tgcgcggcca gctcagccat ctccaccggg agcaggtgcg
     3121 tgaatttggc cgtctctatc agcgcaccgc ggctgatttt gctattgccc accaggaaat
     3181 tcgagacagt catctggtga attatctcaa taacctggtt ggccgggcac acggcaccat
     3241 ttaccgctca aagagttccg gctttaaaga aatctgggaa ttttaccgct attctgcccc
     3301 ggctttattt cgagaaacgt ggcgctatac cctggcggcc ttcctggtgt ttatggtggt
     3361 tggggtattt agcggtgtct ttgtgtttct cgatgatcag tattctcttc gactggcgcc
     3421 cgaagttctg gacatggtca agtccaaaca gaactggacg gaaatgatca acaaaaacaa
     3481 tcaggctgcc tcgtccatga ttttcaccaa taacgccagg gtggcggtgt attcgtttgc
     3541 cactggaatt tttcttgggc tggggtcgct ctattttatg gccaccaacg gcctgcacat
     3601 tggcggggtg accgcgatgt gtatcaagta caaattcact ccgattttga tttttatggc
     3661 agctcatggt gtgtttgagc ttacggcaat ttttattgca ggcggagcgg gttttctgat
     3721 aggtagcgca ttactgatgc ccggcaatct gaggcggggc gaagcactga tcgaacgtgg
     3781 gatcaaagcc attaaattaa tgatgatttg tctgccactg ctggtcgtgg ctggtctcat
     3841 cgaaggcttt ctctcaccac tcaatatttc acccgcttat aagctggcgg tctcaggggc
     3901 gacggcactt ttccttgtgg
//
LOCUS       DAOQDO010000277         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16393_length_3917_cov_2.756862, whole genome shotgun sequence.
ACCESSION   DAOQDO010000277 DAOQDO010000000
VERSION     DAOQDO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16393_length_3917_cov_2.756862"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="PKZ53_05730"
     CDS             <1..1820
                     /locus_tag="PKZ53_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ39969.1"
                     /translation="DAEADVKPRTVRVAGATAREMLIAAAAATWQVESATCETSQGMV
                     IHPPTGRKAAYGELVAAAAKLTPPPSPKVKNPTDFKLLGNRVKRVDGPDIVNGKAAYT
                     ADIRVPGMRFAAVKRSPVLGGKVARWDATKAKKIRGVRNVVQVPTGIAVIADDTWAAF
                     RGVEALEIEWDLGANKDYSSELHWKRTEELSRQEGRVILTQGDRTKAVETAAHKLEEV
                     YAYGFQAHAPVETLTCTVHVRKGECEIWTGTQCANQAQEGVAKQLGLEVGAVKMHVPL
                     LGGAFGRRLQFDYVTEAVEVAKQSPDPVQVIWSRSDDMKHGYFHPASMHRMTGTVDAS
                     GKLTSFGHRLCASLLSVLGPPDFKDPNWHVGYGWGLYDSPYAFPAMHYDFVLVDSPVP
                     SGPWRAVHYPPNVFARESFLDELAHAAKADPVEFRLEYLKGDVAPFQTIRPSLRRVIQ
                     LAAEKAGWGKPLPNGHALGIACNVYHERTTMAQIAEVSVGADGRPKLHRIVCAVECGQ
                     VVNPLGLEGQIESGIVWGLSAAMSGEITVKNGGIVESNYADFGVFRIKDMPRVEVYTV
                     PSTAPPTGIGEQPAPPAAPAVFNAFFAAGGKRIRRLPMK"
     gene            2065..3768
                     /locus_tag="PKZ53_05735"
     CDS             2065..3768
                     /locus_tag="PKZ53_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HNJ39970.1"
                     /translation="MLPRDFLLDTAQRGLKKGIPDELNWFPFDQLPALTWSDTGETIE
                     PEIVSWWLIQGVRKKSPEAILLQEGWPEQIPNSQGEILGKFVLEQWIQQDTQIIEQPD
                     IQTLTEQLTAEFQQLRHFSFLNITPEEEDRRLHDFLTYMIADKTRRGSAIKEKGILSV
                     AGSFGRAALIPLVEKYIDDWQVKRSAQCWALVQMLGTVDDARCIQWLVRTSQAKNRTK
                     SIRQQAQKYLDTLARRKGWSQDDLIDRTIPTLGFDSGGVLKLDFGPRSFSVQIDDDFK
                     LVLKDAEGTILKTLPTPRASDDEKLAKEAKKTLSDLKKQLKELFEFQTTRLWEAMCLR
                     RNWSAEDWTLCFQKHPVMGRLCQRVLWEAVTPHSSVCGFRPLADGTLTDADDNPVELT
                     AEVRVRVAHALSMTEAERAQWQSHLADYEVQTLFPQVAWAPYRLPEKLQKTKELTDFV
                     GHLVEAVVLHGKAAKFGYSRAESDDGLWFWVYQKNLPALGVKVVIEYTGSGDLKEKRT
                     VALKNFYLTSDLSSYGYETKKLELGKIPPVLLSEIWHEVRQLAAAGTGFNPEWKKMCE
                     V"
BASE COUNT          962 a         1039 c         1081 g          835 t
ORIGIN      
        1 gcgacgcgga agccgacgtg aaaccacgga ccgttcgggt cgccggggcc acggcccggg
       61 agatgttgat tgcagcagca gcagccacct ggcaggttga atccgcgacc tgcgaaacat
      121 cacagggaat ggtcatccat cctccaacgg gacgcaaagc ggcgtatggc gaactggttg
      181 cagccgccgc caaactgacg ccgccgcctt ccccgaaagt aaagaatcca actgatttca
      241 agctgcttgg aaatcgggtc aaacgcgttg acggtcccga tatcgtgaac ggaaaagctg
      301 cctacacggc agatattcgc gtgcccggaa tgcggtttgc ggcagtaaaa cggtctccgg
      361 tcctgggcgg aaaagtagcc agatgggacg caaccaaagc caaaaagatc cggggtgtac
      421 gcaacgtcgt gcaggtgcca accggaattg cggtgattgc cgatgacacc tgggcggcct
      481 ttcggggcgt tgaagccctt gaaatcgaat gggacctggg cgcaaacaaa gactattcat
      541 ctgaacttca ctggaaacgg actgaagaat taagccgcca ggaaggccgg gtgattttga
      601 cccagggcga ccggacgaaa gcggttgaaa cagcggcaca taaacttgaa gaagtttacg
      661 cttacgggtt tcaagcccac gcgccggtgg aaaccctgac ctgtacggtt cacgttcgca
      721 aaggcgaatg tgaaatctgg accggcaccc agtgtgccaa tcaggctcag gaaggcgtgg
      781 ccaaacagct tggcctcgaa gttggcgcgg tcaaaatgca cgttccctta ctcggtggcg
      841 cttttggccg gagattgcag tttgattatg tcaccgaagc tgtcgaagtc gccaaacaga
      901 gcccggatcc ggtccaggtc atctggtcac gaagcgatga tatgaaacac ggctattttc
      961 atccggcgtc catgcaccgc atgactggaa cggtggatgc cagcggaaaa ttgaccagtt
     1021 tcggccatcg gctctgtgcc tcattgcttt cggttctggg gccacctgac tttaaggatc
     1081 caaactggca cgttggctat ggatgggggc tctatgattc accctacgca tttccagcca
     1141 tgcactatga ttttgtgctg gttgactcgc cggtaccgtc ggggccgtgg cgggcggtgc
     1201 attacccacc aaatgtcttt gcccgtgaaa gctttctgga tgaactggcg cacgctgcca
     1261 aagccgaccc ggttgaattt cggctggagt atctcaaagg cgatgtcgcg ccgtttcaaa
     1321 ccattcgccc aagtctgcga cgggtaatcc aactcgcggc tgaaaaagcc ggctggggca
     1381 aaccgcttcc caacggccac gcgctgggca tcgcctgcaa cgtctatcac gaacgaacca
     1441 ccatggcgca aattgccgaa gtctcagttg gcgcggatgg cagacccaaa cttcatcgca
     1501 ttgtctgtgc ggtcgaatgc ggtcaggtcg tcaatccact tgggttggaa ggccagattg
     1561 aaagcggtat cgtgtggggg ctctcagccg cgatgtcagg tgaaatcaca gtcaaaaacg
     1621 gcggtatcgt tgaaagcaat tatgcggatt ttggggtgtt tcgaatcaaa gatatgccac
     1681 gggttgaagt ctacaccgtc cccagtaccg cgcctcccac cggcattggc gaacaaccgg
     1741 ctccaccagc ggcaccagcg gtgttcaatg cgttttttgc cgccggaggt aagcgcatcc
     1801 gacggttgcc gatgaagtag gtctgaagaa acggggctga aaattcgcag gctcagggct
     1861 cagggctgaa gaaattgggc tgaagaaatc gggtgagggc ttggggtttt agaacaaaaa
     1921 aggttgactg gttcagtcaa agaccataaa acaaactttt aggctttggt ttcaataccc
     1981 tcagccctga gcccgatttc ttcagccctg agcccgattt cttcagccct gagcccaatt
     2041 tcttcagcct caagccctac ttctatgctt ccccgtgact tcctgctcga cacggcccaa
     2101 cgtggcctga aaaaaggcat tcccgacgaa ctcaactggt ttccctttga tcaacttccg
     2161 gcgctgacct ggagcgatac cggagaaacc atcgaacctg aaattgtttc ctggtggctg
     2221 attcaaggcg ttcgaaagaa aagtccggaa gctatcttgc tccaggaagg ttggcccgaa
     2281 caaatcccca actcgcaggg cgaaatcctg ggaaaatttg ttttagagca atggattcaa
     2341 caagataccc agattatcga gcagcctgac attcagactc tcaccgagca actcaccgct
     2401 gaatttcaac aacttcggca cttttcattt cttaacatca cacccgaaga agaagaccgg
     2461 cggctccatg attttctgac ctatatgatt gccgacaaaa ccagacgcgg cagcgcaatc
     2521 aaggaaaaag gtattctgtc agtcgccggg tcgtttggtc gtgcggcgtt gattccactg
     2581 gttgaaaaat atattgatga ctggcaggtc aaacgttcgg ctcagtgctg ggcactcgtg
     2641 caaatgcttg gaacagttga tgatgcgcgg tgtattcagt ggctggtacg aacttcgcag
     2701 gccaagaatc gaaccaaaag catccgccag caagcccaaa agtatctgga caccctggcc
     2761 agacgcaaag gctggtctca ggatgacctg attgaccgca cgattccgac actggggttt
     2821 gattccggcg gtgtgctaaa gctcgatttt ggcccgcgtt cgttttcagt tcagattgac
     2881 gatgacttca aactggtgct caaggacgca gagggcacca tcctcaaaac cctcccgaca
     2941 ccgcgagcca gcgatgatga aaaactggcc aaagaagcga aaaaaacgct ttcggatctc
     3001 aaaaaacaac tgaaagaact ctttgagttt cagacgactc gtctttggga agcgatgtgt
     3061 ctccggcgta actggtcggc ggaagactgg acgctgtgtt tccaaaagca tccagtgatg
     3121 ggccgactgt gccagcgtgt gctttgggaa gcagtcaccc cgcattcatc ggtctgtgga
     3181 tttcgaccac tggcggatgg aacgctgacg gatgccgatg acaacccggt tgaactcacg
     3241 gctgaggttc gggttcgagt cgcccatgcc ctttcaatga ccgaagccga acgtgctcag
     3301 tggcagtcac atctggccga ctatgaagtg caaaccctct ttccacaggt ggcctgggcg
     3361 ccctatcgcc tgcctgagaa gcttcagaaa accaaagaac tgaccgactt tgtcggccac
     3421 ctggttgaag ctgtcgtgct gcatggcaaa gccgccaaat ttggctatag ccgggctgaa
     3481 tctgacgatg gcctctggtt ttgggtgtat caaaaaaacc tgccggcact cggcgtcaaa
     3541 gtggtgattg aatacaccgg cagcggtgac ttgaaggaaa aacggacagt ggcactcaaa
     3601 aacttttatt tgacttcgga tctctcaagc tatggctacg aaacgaaaaa gctcgaactc
     3661 ggcaagattc cgccagtgct cctgagcgaa atctggcacg aagtccgcca actggcagcc
     3721 gctggaacgg gcttcaaccc ggagtggaag aaaatgtgtg aagtatgagg ttgaaggctg
     3781 aagactttgg gctgaagacg ttgggctgaa gacgttgggc tgaagacaat gagattacaa
     3841 cccctcaatc cgccttcttc agcccgtttt cttcagcccc aagccccaag ccctggtttt
     3901 tactgactgg tatcaat
//
LOCUS       DAOQDO010000278         3912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16425_length_3912_cov_3.406534, whole genome shotgun sequence.
ACCESSION   DAOQDO010000278 DAOQDO010000000
VERSION     DAOQDO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16425_length_3912_cov_3.406534"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3020)
                     /locus_tag="PKZ53_05740"
     CDS             complement(<1..3020)
                     /locus_tag="PKZ53_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39971.1"
                     /translation="MSLDTSILNVGEYFSSHYLESTFSKDVAALADRWKEDGSQAVSR
                     KLQNLAQTYFRAKAQALDESHPERRTDAGDDVRAWHTLLRTALGYGNVQPFDFPVEGG
                     ERFVPAVGRVDRYNKPWLVLCETWFTLPESALRDGQPTEDPLELTPAPTQLCDRVAHL
                     LCAGDWARCIGRLFTEEDAPRWVLFLAGSQILLLDRHTFAQGRYLAFDLDDAFGRRER
                     DTFTHIAAFLALETLCPDGEANEVLLDKLNEQSHRFAHGVTENLQHALREAIELLVNE
                     WAYDRTERQKLPLLRLRPHEIVSGMNVVRDDDGNAEITAEQLKREALAFVYRLLFCLY
                     AEARGGELEILPIDDDTYRLGYSFDSLRDLEQIPLTPTTEDGTYFHEHLKVLFRIIHQ
                     GFHPERETYNTPLAPLLQGEGTLKTFEIRPLTATLFAPESTPLLGRARLSNRCLQQVI
                     RRLSLSKDDRSRTIGRVNYAELGINQLGAVYEGLLSYKGQFADQDLIHVKPASGSFQD
                     KKTTTWFVSKDRLPEFETDEVERLEGGKPRIYTKGTFILHLSGIDREQSASYYTPEVL
                     TRCLVEESLRELLADFGPDDADRILDLKICEPAMGSGAFLNEAADQLAHRYLDLKQKQ
                     TGRTVEPAQYADEVRRVKHFITTRNVYGVDLNATAVELGALSLWLGSVHRLLQYSDET
                     GGRDRYLPGATPWFGLRLRSGNSLIGARRAVWKIDHLKRGKHIGKDGELPRLLAPNEA
                     RRPDEVYHFLVFDEEMIPTHGDKLVRGFQPEACTKAKDWLTRQVKPKWSDSDLKDALT
                     ICDLIDRQWNRFTEQRQEALNETACTATVWPLPAKDPKAIAAGPSLERQERVKANLES
                     SSGSFQRLKLVMDVWCALWFWPLEQVQNLPTRESFLAVARLLLGDEFPKADTWGLIEI
                     RLGFSLSVLQAASASTVPDTGTLALAIPWLGITQRLAAEQSFHHWELTFPEILGPTAP
                     HAGFDLIVGNPPWIQVAWVEATVLSEIEPLLGVQ"
     gene            complement(3049..>3912)
                     /locus_tag="PKZ53_05745"
     CDS             complement(3049..>3912)
                     /locus_tag="PKZ53_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNJ39972.1"
                     /translation="SNPKYLPIEEQGNLLFVHASEDLRRRLGAPDERGDVIFGSTAIP
                     AEAWPEDNQFRLTDDPNQVKSAIQAARNFSGHWSKELLCTDQHPILKWLTERLLMLVK
                     RGECPMVLSNRLDPGEMYFCFIGQVSSLAGAPLVADAHAVSFSKSGHIATISLSEAVE
                     RAGLTSMVNDGRSGNTMVAKTLLPSAVAESRNHLVALSHQHLDRLIQLLRPEERRLKR
                     WKERRRDLLETQIRQLGDTNPQSRKYRRQLDEIDDYVKDRQKNWLDVHFKAAAEPVTQ
                     LVLVIEGRPRT"
BASE COUNT          913 a         1061 c         1062 g          876 t
ORIGIN      
        1 tcttgaaccc caagtaatgg ttcaatttct gataaaaccg ttgcttccac ccaggcaact
       61 tgaatccatg gtggatttcc gacaatcaaa tcaaagccgg catgaggtgc ggtcgggccg
      121 aggatttccg gaaaagtgag ttcccagtga tggaatgatt gttcggcggc aagccgttgg
      181 gtgatgccca gccagggaat cgccagggcc agcgttccgg tatcgggtac cgtcgaggca
      241 gaagccgcct gtaacactga aagcgagaac ccgagccgga tttcaatcag cccccaggta
      301 tcagcctttg ggaattcatc acccagcaac aacctggcga cggccagaaa actttcgcga
      361 gttggcagat tttgaacctg ttccagcggc cagaaccaga gcgcgcacca gacatccatc
      421 accagcttga ggcgttgaaa gctcccgcta gagctttcca ggttagcttt gacacgttcc
      481 tggcgttcga gcgaaggccc ggcagcaatt gctttggggt ctttggccgg gagcggccaa
      541 acggttgccg tacaggcggt ttcattgagt gcctcctggc gttgctcagt gaaccggttc
      601 cactgtctgt caatcaagtc acagatggtg agagcgtctt tgaggtcact atccgaccat
      661 ttcggtttga cctgtctggt aagccagtct ttggcctttg tacaggcttc aggctgaaac
      721 cctctcacga gtttgtcacc gtgggtcggt atcatttcct cgtcaaagac cagaaaatga
      781 tacacctcgt ctggtcggcg agcttcattt ggggctaaca accgtgggag ttcaccatct
      841 ttgccgatat gtttgccgcg tttcaggtgg tcaatcttcc aaacggcgcg ccgggcacca
      901 atgaggctgt tgccactgcg caggcgaagg ccaaaccagg gcgtggcacc gggcagatac
      961 cggtcacgac cgccggtttc gtcactatat tgcaaaagcc gatggacgct gcccagccag
     1021 agcgaaagcg cgccaagttc gacagcggtg gcgttgagat ccacgccata cacattgcgg
     1081 gtggtgatga aatgtttgac ccggcggact tcgtcggcat attgggccgg ttccacggtc
     1141 cggccagtct gtttttgttt caagtcaagg tagcgatggg caagctggtc agcggcttca
     1201 ttgagaaagg cgccgcttcc cattgctggc tcacagattt tcaggtccag tatccggtcg
     1261 gcatcatcag ggccgaaatc ggcaagtaac tcgcggagag attcctcaac cagacaccgg
     1321 gtgaggactt ccggggtgta gtacgatgca ctttgctcac ggtcaatccc gctcagatgc
     1381 aaaataaacg tgcctttggt gtaaatgcgc ggtttcccac cctctaaccg ctcaacttcg
     1441 tcagtttcaa attctggcaa ccggtctttg ctgacaaacc aggtcgtggt ttttttgtct
     1501 tgaaaacttc ccgaagctgg tttgacgtga attaaatcct ggtcggcaaa ctgacctttg
     1561 taggaaagta acccttcata caccgcgccc aactgattga taccaagttc ggcatagttt
     1621 acccggccaa tggtgcgtga gcgatcatct ttactgagtg agagccgccg aatcacctgt
     1681 tgcaaacatc ggtttgacaa ccgtgcgcga ccaagcaacg gggtgctctc cggagcaaaa
     1741 agcgttgccg tcaacgggcg aatctcaaag gttttcagtg ttccctctcc ctgcaggagt
     1801 ggtgccaggg gcgtgttgta cgtttcgcgt tcagggtgaa acccctgatg gataatccga
     1861 aacaacactt tcaggtgctc gtgaaaatat gtaccgtcct ccgttgtcgg agtgagagga
     1921 atctgctcaa ggtcgcggag cgaatcaaaa ctgtaaccaa gccgataggt atcgtcatca
     1981 attgggagga tttcaagctc accgccccgc gcctcggcat acaaacaaaa cagtaaccgg
     2041 tagacaaacg ccagtgcctc gcgttttaac tgttcggcgg taatctcggc gttgccatca
     2101 tcatcccgaa ccacattcat cccgctcaca atctcgtgcg gacgcaggcg caacagcggc
     2161 aatttttgac gttcggtgcg gtcataggcc cattcattga ccaggagttc aatggcttcg
     2221 cgcaatgcgt gctgtaaatt ctcagtcact ccgtgggcaa atcggtggct ttgctcattg
     2281 agtttatcaa gtaaaacttc gttggcttcg ccgtctggac ataacgtctc aagcgccaga
     2341 aaggcggcaa tgtgcgtaaa ggtgtcgcgt tcacggcgtc cgaaggcatc atcaaggtca
     2401 aaggcgagat accgtccctg ggcaaaggta tgccggtcca acagcaagat ctggcttcca
     2461 gccagaaaca acacccagcg tggggcatct tcttcagtaa acaaccgtcc aatgcaccgt
     2521 gcccaatcac cggcacagag gagatgcgcc acccgatcac acaactgagt cggagctggt
     2581 gtcagttcga gcggatcctc tgtcggctgg ccatctctca gggcgctttc aggcagcgtg
     2641 aaccaggtct cgcaaagcac cagccagggt ttgttgtagc gatccacgcg ccccacggct
     2701 ggaacaaatc tttcgccccc ctcgaccggg aaatcaaagg gttgaacgtt gccatacccc
     2761 aatgccgtcc ggagcagcgt atgccaggcc cggacatcgt caccggcatc agtacggcgt
     2821 tcaggatgag attcatcaag ggcctgggcc ttggcgcgaa agtaagtctg ggctaaattt
     2881 tggagcttcc gcgaaacagc ctgggaaccg tcttccttcc agcgatcagc cagggccgcc
     2941 acatcttttg aaaaggtgct ttccagatag tgcgatgaga aatattcacc aacattgaga
     3001 atagaggtat cgagcgacat gcagatgaaa ctccacaggc aaaagggttc aggtacgtgg
     3061 tcggccttca atcactaaaa ccagttgggt aacgggttcg gcagcagctt tgaaatgcac
     3121 atccagccag tttttttgac ggtctttgac ataatcatca atttcatcaa gctggcggcg
     3181 atattttcgc gattgtgggt tggtatcgcc aagttgccgg atctgggttt cgagcaaatc
     3241 ccgacgacgc tccttccagc gtttgagacg gcgttcttcc ggtcgaagca actggatcaa
     3301 gcgatccaga tgctgatgag acagagccac aagatgattg cgactttccg cgacggcgga
     3361 aggcaaaagc gttttcgcca ccattgtatt tccactgcgg ccatcattga ccatcgaggt
     3421 cagtccagcc cgttcaacgg cttcagaaag cgaaatcgtc gcaatgtgac ctgacttcga
     3481 aaacgacacg gcatgggcat ctgcgaccag gggggcacct gccagcgagc tgacctgacc
     3541 gataaagcaa aaatacattt caccagggtc taaccggttc gagagcacca ttggacattc
     3601 accgcgcttg acgagcatca gcaggcgttc ggtaagccat ttcaagatcg ggtgctggtc
     3661 ggtgcaaaga agttcttttg accagtggcc tgaaaaattg cgagcagcct gaatcgccga
     3721 tttgacctga ttcgggtcgt cagtaaggcg aaactggtta tcctcgggcc aggcttcagc
     3781 cggtatagcg gtggaaccaa aaataacgtc gccccgctca tctggggcgc ccagccgccg
     3841 ccgcaaatcc tctgaagcat ggacaaacag caggtttccc tgttcctcaa tcggcaggta
     3901 ttttgggttg ga
//
LOCUS       DAOQDO010000279         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16450_length_3909_cov_3.231448, whole genome shotgun sequence.
ACCESSION   DAOQDO010000279 DAOQDO010000000
VERSION     DAOQDO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16450_length_3909_cov_3.231448"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKZ53_05750"
     CDS             complement(<1..221)
                     /locus_tag="PKZ53_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39973.1"
                     /translation="MLITMCLLDGNAVLAQSPDVRIVEDTGLKLMTNRPKPKKARRMN
                     RRYRVVSPPIAGKPAATALELGVTLWRLRP"
     gene            complement(360..2189)
                     /locus_tag="PKZ53_05755"
     CDS             complement(360..2189)
                     /locus_tag="PKZ53_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ39974.1"
                     /translation="MTPHRWQQVKTVLNEVLEQPLDQQLVYLERVCGDDLELRREVES
                     LLECSEDIEPDFLVPVSESSDGMRDEPTVSTTPEKGGKQIPVCEPGTVIDGRYEIERE
                     LGRGGFSLVYLAQERLYQAAGGPPILRPVVVKCLLSENVKSEWLHRKFQHEIEALSQI
                     DHPHVVGLLKAGELPDGQPYLVMPYIKGETLRSVIAPPGMPVSSALGIFRQLCEALTA
                     VHRHGIIHRDLKPENIMLQPQATDEFQVKLIDFGIARLSVSQVGDTTHRSTAVGTLRY
                     MSPEQLLEGKTSVQSDIYSLGVILVELLTGRRPFDSKAPTPYSVVMELITQQRNGLPA
                     LDGGIPEAVKLVLARALAFEPEHRWPSVAEFQSRLLESFDQSSVNPPVKLKPSGRPLR
                     WSRAILATMVCGGLGAGVWWYETAGFKSPASVKSVPPVVSGEVQLGWSLTVEKLKNGQ
                     PDGPTFELVKEAVFDHATAYRVRINLTPTQPGHVYVINEGPGKAAAIEYVKVFPTPTA
                     NQNQSAVTAHRTVRIPERDWLEFTGGAGTETFWLIWSAKPQPTLETLPVGRLASPDLA
                     DSVRVFLGKTTGAQSEAAASTLDRTVIRSTGDLIVYPLRFERR"
     gene            2483..3052
                     /locus_tag="PKZ53_05760"
     CDS             2483..3052
                     /locus_tag="PKZ53_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ39975.1"
                     /translation="MDGPSSPNVTELLLAWNAGDQEAQAELIAVIYDELRRLAAGYLH
                     RERPNHTLQPTALVNEAFMRLIDGPQVTWQNRAHFFGIAANAMRQILVDHARKRQAQK
                     RGSGEAPLSLDETFGVFQQRAIDLVRLDDALNELAELDPTQAHIVELRFFAGLTIEET
                     AEALGISPATIGREWTSARLWLRRELTRS"
     gene            3121..>3909
                     /locus_tag="PKZ53_05765"
     CDS             3121..>3909
                     /locus_tag="PKZ53_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39976.1"
                     /translation="MCVVLSISVTGSQITTHPPQIQPSPEPALSRLKPVEREMKGGET
                     HRYEVNVPAGHCLNVKVDQNKLNVMLRVVGPDGKEMTMADYTQEFTGPDFLTFVADLA
                     DTYYLELKPFDNQAEPGRYTVQILELRPATQDDRDRMLAQQRFQEALRTHHKQTKQAK
                     EAALQELELIRGIFHRVQDPLGESNALDWMAKIIGEFGRTKEAKELNLQALALKKAAG
                     FRLNAATTLNNLAILHNQLGEFQEALTAYEEALKISIEFDDKESQ"
BASE COUNT          942 a         1072 c          991 g          904 t
ORIGIN      
        1 gggcgcaatc gccagagcgt gacgccgagt tcaagtgctg ttgctgctgg ctttccagct
       61 atcggaggcg agacaacccg gtatcggcgg ttcattcgac gcgctttctt gggctttggt
      121 cggttcgtca tcaatttcaa gcccgtatct tcgacaattc gcacatcggg ggattgagcc
      181 agaacggcgt tgccatccaa taaacacatt gtaatcagca tgatagaaaa gaaaacctga
      241 aacgaatttt tcatatgggt atttggaatg ttcctgactc tgacaaaata acaagctgac
      301 aaggtgattt ttttcatccc tcatcccttc aaaacccctg acctctgccc cctacctgac
      361 tatcgccgtt caaaccggag cggatacaca atcaaatctc cagtggatcg aatcacagtg
      421 cgatccagag ttgaagccgc agcctcgctt tgtgctccag ttgtcttccc aagaaacact
      481 cgaactgaat cagccaaatc tggcgacgca agcctcccca cggggagcgt ttccagcgtg
      541 ggttgtggtt tggctgacca gataagccag aaggtctcgg ttccggctcc gccagtaaat
      601 tcgagccagt cacgttccgg aatgcgaacc gtgcggtgtg ccgtcaccgc cgattggttc
      661 tggttggcgg tcggcgttgg gaagaccttt acatattcga tggcggcagc ttttccagga
      721 ccttcattga tgacatacac atggcccggt tgggttggcg tcagattgat tcgcacccgg
      781 taggcagtgg cgtgatcaaa gacggcttct ttcacgagtt caaaggtggg gccgtctggc
      841 tgaccgtttt tcaatttttc gactgtgagc gaccagccaa gctgaacttc gccagaaaca
      901 actggcggaa ctgactttac agatgccgga gacttgaaac cagccgtctc ataccaccac
      961 acaccagccc caagaccgcc acacaccatg gttgccagaa tggcacgtga ccaccgcaag
     1021 ggcctgccag atggcttcag cttaaccggt ggatttactg acgactggtc aaagctttcg
     1081 agaagccggg attgaaattc cgcaacggaa ggccagcgat gttcgggttc aaacgccagc
     1141 gccctggcca gtaccagttt gacagcctct gggatgccac catccagtgc cggcagtcca
     1201 tttcgttgct gggtaattaa ctccatcacg actgaatacg gcgtcggagc ttttgaatca
     1261 aaaggccgcc gtccggttaa aagctcaacc agaatgaccc caagtgagta gatatcgctc
     1321 tgcacgctgg ttttgccttc gagcagttgt tccggcgaca tgtagcggag cgttcccaca
     1381 gcagtggacc gatgtgtcgt gtccccaact tgcgaaacgc tgagccgggc aattccgaaa
     1441 tcaatcaact tcacctgaaa ttcatcagtt gcctgcggct ggagcatgat gttttccggc
     1501 ttcaggtcgc gatgaataat tccatgcctg tgaaccgctg tcagtgcctc acacagttgc
     1561 cgaaaaatgc ccagggcgct ggagactggc attcctggag gagcaatcac cgaccggagt
     1621 gtctctcctt taatatatgg catgaccaga tagggctggc cgtcgggcag ctcaccggct
     1681 ttgagcaacc ccaccacatg cggatgatca atctgggaca gcgcttcgat ctcgtgctga
     1741 aacttgcggt gaagccactc tgatttcacg ttttcggaaa gcaaacattt gacaacgacg
     1801 ggccgcaaaa tcggagggcc tcccgcagcc tgatagagcc gttcctgtgc cagatacacc
     1861 aggctaaagc cgccacgccc aagttcgcgt tcaatttcat accgcccatc aatgacggtt
     1921 cctggttcac agaccgggat ctgttttcca cctttttcag gagtggtcga aactgtcggt
     1981 tcatctctca tgccatctga agactctgaa accggcacta aaaaatccgg ctcgatatcc
     2041 tcagaacatt caagcagtga ctcgacctca cggcgcaatt ccaggtcatc tccacaaacc
     2101 cgctccagat agaccagttg ctggtccagc ggttgttcca ggacttcgtt gagtacggtt
     2161 ttgacctgtt gccagcggtg tggagtcata ggaaagggct tggggcttgg ggctaagggc
     2221 tgaagaaaaa ccagggttgc ggggttcagg gttgaaaaac cagggttcgg ggttgcgggg
     2281 ttgcggggtt gcggggtttt cgaactttgg tccttttcgt cccttcagtc ctttttgtcc
     2341 ttttccaccc tgaaccctga accctgaacc ctgaaccctg aattcagttt tcatcatttt
     2401 tcatttgcgg tagagtgacg aggctgatat tccctcatgc gcaaacttaa tccgatttta
     2461 ctcaaaccga aaattatttt ttatggatgg cccttcttca ccaaatgtga ctgagctgct
     2521 gctcgcctgg aatgctggtg accaggaggc acaggccgaa ttgattgcag tcatttatga
     2581 cgaacttcga cgcctggcgg ctgggtatct ccaccgtgaa cggccaaacc ataccctgca
     2641 accgaccgcg ctggtcaacg aagccttcat gcggctcatt gatggtccac aggtcacctg
     2701 gcaaaaccgg gcccattttt tcggaattgc ggctaacgcc atgcgtcaga ttctggttga
     2761 ccacgcccgc aaacgacagg cacagaaacg cggcagcggc gaagcaccac tttccctgga
     2821 tgaaaccttc ggcgtgtttc aacaacgggc gattgacctg gttcgactcg atgatgccct
     2881 caacgaatta gccgaacttg atccgacgca ggcccacatt gtggaattgc gtttttttgc
     2941 cggcttgaca atcgaggaaa ccgccgaagc actcggcatt tcaccggcaa ccattggacg
     3001 ggaatggact tcggcccgtc tctggctacg aagggagtta acccgctcat gatgaaattc
     3061 agccagtcag tactacctgt catcgtgggc agatctttat gcttgattcc tggctggttg
     3121 gtgtgtgtcg ttctgagtat ttccgtcaca gggtcacaga tcacaacaca tccgccccaa
     3181 attcaaccct cacccgagcc cgccctcagc cggttaaaac cagttgaacg tgaaatgaaa
     3241 ggcggcgaaa cccaccgcta tgaagtaaat gtgcctgccg ggcattgcct caatgtcaaa
     3301 gttgaccaaa acaaactcaa tgtcatgctg cgcgtggtcg gacctgacgg caaagaaatg
     3361 accatggctg attacacaca ggaattcacc gggccggatt ttctgacctt tgttgccgat
     3421 cttgccgaca cctattacct ggaattgaaa ccatttgaca atcaggctga acctggacgc
     3481 tataccgttc aaatcctgga acttcgcccg gcaacccagg atgaccgtga ccggatgctg
     3541 gcccaacaga gattccagga agcactccga actcatcaca aacaaaccaa acaagcgaaa
     3601 gaagccgccc tccaggaact tgagttaatc cgtgggattt ttcaccgggt tcaagatccg
     3661 ctgggtgaat ccaatgccct ggactggatg gcaaaaatta ttggggagtt tggtcgcacc
     3721 aaagaagcca aagagttgaa tttgcaggca ctggcgttga aaaaagccgc tgggttccga
     3781 ctcaatgcag ccaccacact caacaatctg gcaattttac ataaccagct tggcgaattt
     3841 caggaggcac tcacggccta tgaagaagcc ctgaaaatct cgattgaatt tgatgataaa
     3901 gaatcccag
//
LOCUS       DAOQDO010000280         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16498_length_3903_cov_2.881237, whole genome shotgun sequence.
ACCESSION   DAOQDO010000280 DAOQDO010000000
VERSION     DAOQDO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16498_length_3903_cov_2.881237"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2734)
                     /locus_tag="PKZ53_05770"
     CDS             complement(<1..2734)
                     /locus_tag="PKZ53_05770"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ39977.1"
                     /translation="MARHFVCPLARYSLTGIILISLLATSTVKTTLVKAQSGTQSSQP
                     ATERLRPDIPVERTLNGGEHHRFTLSLQANDYLKLVVYQKGIDVIVRLLSVEERKLLE
                     VDSPNGTQGPEALAFIADTAGTYYLEVASLEPKASPGKYEIKVELVRPATAQDRSELE
                     VKSRLQKAQTLRQAGKLDDGLSLATQALETSEKILGTEHPLVSDSLQILGALYKSKGD
                     AATAESNYRRALTLLEKRLGPEHSDVATTLNSLALVFYERGEYAKAGPLFERALAIRE
                     KISEPSSLRTSLTNLASNSYFQEKYAQAERLFIRAVAIGEQTLGSENLSVAANLFNLG
                     LIYKETGAFAQAEPLFRRALAIREKQLGPENPRVAKDLNSLAELYQAQGDYTTAEPLF
                     VRALAIHEKALGPEHPDVAVGLNNLALLYEDKGDSVRAEDLYLRGLAILEKSRKADPS
                     WMASILSNLANLHQARGNYAQAEAEYRQVLAILEKSLGTEHPKVAICLNNLALLKTAT
                     REYEAAEKLYDRVLAIREKIFGPDHPKVAFTLFNLAGLYGKKGDLAKAKTLYQQALAL
                     QERALAPNHPELAQTLYNLAALCQADGTLAQAAGYLSRSNEVTERDLIRNLVLGSERQ
                     KLLYLDTTSLKTDLTVSFHLQAAPQNPEALQAAMAVVLRRKGRALDATANTIETLRRR
                     ATPEDQALLDELTRTRQFQSTLTLKGQSNQTQAAYLAELDALEKKAEDLEAKISARSA
                     EFQVQHQPVSIENIQKAIPPDAILIEFVRYKSYDPKGRTFGASRYAAYTLDRSGALNW
                     ADLGEAGAVDNLASEFRKTVRRPASSKAIGQTTSPTPPNFQILAQELFRRVIQPIRKG
                     IGTKKQLLIAPDGELNLIPFAALMNETQKYLVETNTITYLTSGRDLLRLQVKIDS"
     gene            complement(2906..>3903)
                     /locus_tag="PKZ53_05775"
     CDS             complement(2906..>3903)
                     /locus_tag="PKZ53_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ39978.1"
                     /translation="IPFSREISSAAQELDRLVMQPVRNLIGPARHLLISPDGTLHLIP
                     FSALVDQNGKFLVETYTLTYLTSGRDLLRLKVKLPAQESAVIIANPNYGNGAGPILAG
                     HPLDPLPPLPETAREGKRIQSLLKRADFVSGRQATEHFVKSIRRPEVLHIATHGYFLA
                     DTRQEIAGEATRDLKLQTVGDLETWRIENPLLRSWLFFSGANQGGQADNDGTMTALEA
                     AQLDLWGTKLVVLSACETGVGETKNGDGVFGLRRALVLAGSESQMISLWAVSDRATRE
                     LMVDYYTRLRTGEGRSQALRNVQLKMLKNPKYQHPFYWAAFIQSGEWARLDGVRK"
BASE COUNT          784 a          996 c         1096 g         1027 t
ORIGIN      
        1 ggctgtcaat tttcacttgc agtcgtaaca aatcccgacc actcgtcaaa taggtgatgg
       61 tgttggtttc gaccaggtat ttctgggttt cattcatcag cgcggcaaag ggaatcaggt
      121 tcagctctcc gtcaggcgca atcaggagct gctttttggt accgatacct ttgcgaattg
      181 gctgtatcac tcggcggaat aattcctgtg ccagtatttg aaaattcggt ggggtggggc
      241 tggttgtctg cccgatggct ttgctcgatg ccggtcgccg aactgttttg cgaaactcac
      301 tggccagatt gtcaacggct cctgcttcac ccaggtcggc ccagttgagg gcgccgctgc
      361 ggtcgagggt ataggccgca taacgcgaag caccaaaggt tcgacctttg gggtcatagg
      421 atttatatct gacaaattca atcagaatgg catctggcgg aatcgctttc tggatattct
      481 cgatggaaac tggttgatgt tggacttgaa attccgcgct ccgggcactg attttggctt
      541 ccagatcctc ggcctttttt tcaagtgcgt ccagttctgc cagatatgcc gcctgggtct
      601 ggttgctctg gcctttgagt gtcaaggtgg attgaaactg cctggttcgg gtcagttcgt
      661 ccaatagtgc ctgatcttcg ggggtggccc ggcggcggag ggtctcaatt gtattggcgg
      721 ttgcatccag tgcccgtcct ttgcgccgga gtaccaccgc cattgccgcc tgcaacgctt
      781 ctggattctg cggtgccgcc tggagatgaa acgagacggt gagatcagtt ttcagggacg
      841 tcgtgtcaag gtacagcagt ttttgacgtt ctgaccccag gaccagattt cgaatgagat
      901 cacgttcggt cacttcattg cttcgtgaga gataaccagc cgcctgagcc agtgtgccgt
      961 cggcctggca caaagccgcc agattataga gtgtctgggc cagttccggg tggtttggag
     1021 ccagggcacg ttcctggagc gccagggcct gctggtacaa ggtttttgcc tttgccagat
     1081 ctcctttttt tccatagagc ccagccagat taaacaaagt aaaggccact ttggggtggt
     1141 ccggcccaaa tattttttcc cgaatcgcca gtacccggtc atagagtttc tctgccgctt
     1201 catactctct ggtggctgtt ttcagtaacg ccaggttatt gaggcagatc gcgactttgg
     1261 gatgttcagt gcccaatgac ttttccagaa tggccagcac ctgacggtat tctgcttcag
     1321 cctgggcata attacctctc gcctggtgca aattggcgag gttactgagg atgctggcca
     1381 tccaggatgg gtcggctttt cgagatttct ccaaaatcgc cagtccccgg aggtagagat
     1441 cctcagcccg taccgaatcg cccttgtcct catagaggag tgccagattg ttgagaccaa
     1501 cggcgacatc cggatgctct ggccccaatg ctttttcatg aatggccagt gcgcggacaa
     1561 acagtggttc ggcggtggtg taatccccct gtgcctggta cagctcggcc agactgttga
     1621 gatctttggc taccctggga ttttccggtc ccagttgctt ttcacgaatg gccagggccc
     1681 ggcgaaataa cggctcggcc tgggcaaaag cgccagtctc tttgtagatc aaccccaggt
     1741 taaacaggtt agccgcaact gacaggtttt ctgaccccag ggtttgctct ccaatcgcca
     1801 ctgcccgaat gaacaaccgt tctgcctggg catatttttc ctggaaataa gagttcgacg
     1861 ccagattagt gaggctggtt ctgagactgg acggctctga tattttttcc cgaatggcga
     1921 gtgcccgttc aaacagcggc ccggctttcg cgtattcgcc acgctcataa aagaccaggg
     1981 ccaggctgtt gagtgttgtg gcaacgtccg aatgttctgg accgagtcgt ttttcaagca
     2041 gggtcagtgc ccggcgatag tttgactcgg ctgtggccgc gtcgcctttg cttttataga
     2101 gcgcccccag gatttggagg ctgtctgaaa ccagcgggtg ctcggtgccc aggatttttt
     2161 cactggtttc aagggcctgg gtggcgagag atagtccatc atcaagcttg ccggcctgac
     2221 gaagcgtttg ggctttttga agccggcttt tgacttcaag ttcactccgg tcctgagctg
     2281 tggctggccg taccagttca actttgattt catatttgcc tggcgaagct ttgggttcca
     2341 gtgacgccac ttccagatag taggtgcctg cagtgtctgc aatgaacgcc agcgcctcag
     2401 gtccctgcgt tccgttcgga ctgtctactt ccaggagttt tctttcctcc acgctcagca
     2461 gccgaacaat gacatcaatt cctttctgat acaccaccag tttcagatag tcattggcct
     2521 gcaaggaaag tgtaaaccgg tgatgttcac cgccattgag ggttcgttcc actggaatgt
     2581 ccgggcggag gcgttcagtt gccggttgtg aagattgggt accggattgg gctttgacca
     2641 gagtggtctt caccgtcgaa gtcgcaagca acgaaatcag gatgatgcca gtgagcgaat
     2701 atcttgccag ggggcagacg aaatgacgtg ccataaggtt ctcgatgtgg aagaaaatga
     2761 agattgaaga gaagctggtt aggggtcaaa agtcaggagt cagggttcag ggttcagggt
     2821 tcagggttca gggttttcga aacctatgtc ctttatactc cttatgtctt tttcgtcttt
     2881 tcacaccttg atccctgttt cctcctcact ttctgacgcc gtccagtctg gcccattcgc
     2941 ccgattggat aaatgctgcc cagtagaatg ggtgctggta ctttgggttt ttgagcattt
     3001 tgagctgcac attgcgcagg gcctgggagc gaccttcgcc agttctcagc cgggtatagt
     3061 agtcaaccat caattcacgt gttgcccgat ctgacaccgc ccacaggctg atcatctggc
     3121 tttcgctccc ggccaggacc agagcgcggc gcaacccaaa gacaccatcc ccatttttgg
     3181 tttctccgac tccggtttca caggctgaga ggacgaccag tttggtgccc cacaaatcga
     3241 gttgggcggc ttcgagcgcg gtcattgtgc catcattgtc ggcctgtcca ccctgattgg
     3301 caccgctaaa aaacaaccac gacctcagaa gcgggttttc aatccgccag gtttcaagat
     3361 ctcctacggt ttgaagtttt aaatcacggg ttgcttcacc cgcaatttcc tggcgggtgt
     3421 ctgctagaaa atagccgtga gttgcgatat ggagcacttc aggtcgccga atggatttga
     3481 caaagtgttc cgttgcctgg cgtccagaaa caaagtcggc tcttttcaaa agggactgaa
     3541 tcctctttcc ttctcgggct gtttcgggca acggtggcaa cgggtcaagc gggtgaccgg
     3601 ccaggatcgg tccggcgccg ttcccataat tggggttggc aatgatgacg gctgattcct
     3661 gagccgggag tttgactttg agccggagca ggtcacgtcc gctggtgaga taggtcaagg
     3721 tataggtttc aaccagaaat ttcccgttct gatcaaccag ggctgagaag ggaatcagat
     3781 gtaaggtgcc atctggtgaa atcagtaagt gcctggctgg tccaatcagg tttcgcactg
     3841 gctgcatgac gagtcggtca agttcctgtg ctgctgaact gatttctctg gaaaagggaa
     3901 tcg
//
LOCUS       DAOQDO010000281         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16506_length_3902_cov_2.665714, whole genome shotgun sequence.
ACCESSION   DAOQDO010000281 DAOQDO010000000
VERSION     DAOQDO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16506_length_3902_cov_2.665714"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKZ53_05780"
     CDS             complement(<1..705)
                     /locus_tag="PKZ53_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ39979.1"
                     /translation="MFEDPEIVLEFLTESNENLARLDQEMVELEQHPGDKNLLASVFR
                     TIHTIKGTCGFLGFSRLESLAHAGESLLSQLRDGQLALTPERTSGLLSMVDAIRQTLT
                     CIEVEKNEGTTDYTPIIERLTRLREPAGDTPPPAGLPEIKAVPLPPPSPALPDLSLKP
                     AVRPPADEYDPQPGHLNLFEINTVAGVFGGDLASAQPVVDGRTSVADTNIRVDVGLLD
                     KLMNLVGELVLARNQIL"
     gene            complement(717..1802)
                     /locus_tag="PKZ53_05785"
     CDS             complement(717..1802)
                     /locus_tag="PKZ53_05785"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNJ39980.1"
                     /translation="MASKIRILLIDDSVVVRKVLTEILARDPEILVVGSAANGSIGLA
                     KIPQLNPDLVVLDLEMPEMDGLETLTRLRRVYPKLPVIMFSSLTERGAVATLQALEFG
                     ANDYVTKPSTQVSNAGQYISEQLIPKIKALWAKVNLTGLPALIPPVSNGHQPLIKKQV
                     PPAHRRVDIVAIGVSTGGPNALALVIPEIPKDFPVPIVVVQHMPPIFTRMLAESLARK
                     SQVAVVEAQSGDYLHPGGVWIAPGDFHMVVGQGARGVRIELNQGPPENSCRPAVDPLF
                     RSVAQVYGYHSLGVVMTGMGQDGLTGSELIQAAQGQVWAQDEATSVVWGMPGFVSKAG
                     LADKIIPLDQIAFEIIRKVSEHRSSCQ"
     gene            1783..2085
                     /locus_tag="PKZ53_05790"
     CDS             1783..2085
                     /locus_tag="PKZ53_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39981.1"
                     /translation="MRILLAMRLFQREQTLFLSGYLSATEMPELLKPGNQVRCLVQMV
                     KAAGFLAGGELSWHRAAPICVQGTGKRAQRLAISIKNLENLMSGVRQVCEIPTDPK"
     gene            complement(2254..2700)
                     /locus_tag="PKZ53_05795"
     CDS             complement(2254..2700)
                     /locus_tag="PKZ53_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39982.1"
                     /translation="MDTYELDNDLRISVQTLLTSQLDDLNLLFLLAGWQLLAAKDRNF
                     DLVGEWSTQRSEVTHRLGTYQRQLLGLFQNYPFLRNTVASSVVAEDTRQLSLKIQRLD
                     QETIDCLVSERKAILTEKAQFDQVQNQLNRFGNTPGAKSIALDHSG"
     gene            complement(2678..3172)
                     /gene="fliS"
                     /locus_tag="PKZ53_05800"
     CDS             complement(2678..3172)
                     /gene="fliS"
                     /locus_tag="PKZ53_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNJ39983.1"
                     /translation="MYGGKKAISAYQQMTNLESNPLRQVVMLFDGAMKFLNLAASDIE
                     LGNLTAKAEHIDRAVNILSYLQSILDFDRGGEIARIYDRLYTSVTSMVVKASLTLDAT
                     TLRQAAQFLSPVREAWATNADLSDQRPAPVSAPTSHVPAPTPLTHHQPPLSGYSNYGH
                     LRTR"
     gene            3477..>3902
                     /locus_tag="PKZ53_05805"
     CDS             3477..>3902
                     /locus_tag="PKZ53_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39984.1"
                     /translation="MTILFFGCLFVASPLTVAAQQAAAIPPSKLAATVEEDFRAGMSG
                     NQDAFDRAMKSTEELLAGNPAHAEALVWHGSGLVSKSGKAFMAGDFQQGSYFMDKGLA
                     EMTKAVELEPENVHVRFTRGSTLLVLSKVFPIPDTAQDCL"
BASE COUNT          889 a         1007 c         1054 g          952 t
ORIGIN      
        1 cagaatctgg ttgcgtgcca ggacgagttc gccgaccaga ttcatcagct tgtcgagcaa
       61 tcccacatca acccgaatat tggtgtcggc aacgctggtt cgtccatcta caaccggctg
      121 ggcactggcc aggtcacctc caaagactcc cgcgacagta ttgatttcaa acagattgag
      181 atgacccggt tgcggatcat actcgtcagc gggaggccgt acggctggct tgagtgacag
      241 gtctggtagt gctggcgatg gtggcggcaa cgggaccgct tttatctcag ggagtcccgc
      301 cggcggtggt gtgtcacctg ctggttctcg taaccgggtc aggcgttcaa taatgggtgt
      361 gtaatcagtg gttccttcat ttttttcaac ctcgatacag gtcagcgttt gccgaatggc
      421 gtccaccatt gacagcaacc ccgaagttct ttcaggggtt aaggccagtt gtccgtcccg
      481 aagttgactg agcaggcttt ccccggcgtg cgccagtgat tccagcctgg aaaaaccaag
      541 aaatccgcag gtgcccttga tggtatgaat cgtgcgaaac acgctggcca gcaggttttt
      601 gtcgccaggg tgctgttcga gttcgaccat ttcctgatct aatcgggcca ggttttcatt
      661 actttcggtt aaaaactcca gtacaatttc cggatcttca aacataggtt ccaccttcac
      721 tgacagcttg agcggtgctc gctcactttc cgaatgattt caaaagcaat ttgatccagc
      781 ggaataattt tatcggccag cccggccttg ctgacaaatc caggcattcc ccaaacgacg
      841 ctggtggctt catcctgagc ccaaacctga ccctgggccg cctgtatcag ctcgcttcca
      901 gtcaatccat cttgccccat gccggtcatt accacgccca atgaatgata cccgtacacc
      961 tgggcgacgg atcgaaataa cggatcaacg gctggccggc acgagttttc cggcggcccc
     1021 tggttgagtt caattctgac cccacgggca ccctgcccca cgaccatatg aaaatcaccg
     1081 ggtgcaatcc agacgccgcc cggatgcaga taatcaccag actgggcttc aaccacggcc
     1141 acctgtgatt ttcgagccag gctttcggcc agcatcctgg taaaaatcgg cggcatatgc
     1201 tgaacaacca caattggaac ggggaaatct tttggaattt ccgggatgac gagggccagc
     1261 gcattggggc cgcccgttga aacgccgatg gcgacgatat caacccgccg atgggctggc
     1321 ggcacctgct ttttgatgag gggttgatgg ccgttggaca ctggcggtat cagcgctgga
     1381 agcccggtca ggttgacttt ggcccagagg gctttgatct ttgggatgag ctgttcgcta
     1441 atatattgac ccgcgttact tacctgggtg gaaggtttgg tgacatagtc gttggcgcca
     1501 aattcaagtg cctggagcgt tgccaccgca ccgcgttcag tcagactgct aaacataatg
     1561 actggaagtt tgggatagac gcggcgcagt cgggttaatg tttccagacc atccatttca
     1621 ggcatttcca ggtcaagcac aaccagatca ggattgagct gggggatttt ggccagtccg
     1681 attgatccat ttgctgcact gcccaccacc agaatctctg ggtctctggc aaggatttcc
     1741 gtcaacacct tgcgaaccac aaccgaatca tcaatgagga gaatgcgaat tttgcttgcc
     1801 atgaggttat tccagcgcga acagactctt ttcctgtcgg gatacctctc cgcaactgag
     1861 atgccagagt tactcaagcc cggcaatcag gttcggtgcc tggttcaaat ggttaaagcg
     1921 gctggttttt tggcaggagg ggagctttcc tggcacaggg cagcaccaat ttgtgttcag
     1981 ggaactggga agcgtgctca gcggctcgcc atcagcatca aaaatctgga aaatttgatg
     2041 tcaggtgtcc gacaggtatg cgaaatcccg acagacccga aatgaagggt gttcatctat
     2101 aaagtttcac ggaaagaaac cacgagccat accgggctac gcagaaaata acgccatctt
     2161 tttcaatggt ttctggaatc tcaaccggat ggctgcccgc gaaatgttta tttgaaaggc
     2221 ggatagcggc aggctgattc agtcgccggg ccctcagcca ctgtgatcca gggcaatgga
     2281 tttcgctccg ggtgtgttcc caaaccgatt tagctggttt tgaacctgat caaactgagc
     2341 tttttcagtc aaaattgcct tccgttcaga gaccagacag tcaatcgttt cctggtcaag
     2401 ccgttggatt ttgagtgaca gttgtcgagt gtcttcggcc accacactcg atgcaacagt
     2461 gttgcgcaag aaagggtagt tttgaaacag ccccaataat tgacgttgat atgttcccag
     2521 acgatgggtg acctctgatc gttgggttga ccattcgccg accaggtcaa aattccggtc
     2581 ctttgccgcc aataactgcc agccagccag caggaacagg aggttcagat cgtcgagctg
     2641 tgaggtcagc agggtctgta ctgagatcct caaatcgtta tcgagttcgt aggtgtccat
     2701 aatttgaata tcctgaaagc ggtggttgat gatgtgtgag cggtgttggc gctggtacgt
     2761 ggctggttgg tgctgacact ggtgctggtc gctggtcgct caggtcggca ttggtggccc
     2821 aggcttcacg caccggagag aggaattgag cggcctggcg caacgtcgtg gcatccagcg
     2881 tcagactggc cttgaccacc attgaggtca cactggtgta aagccgatca taaatgcgtg
     2941 caatttcccc gccacgatca aagtcgagga ttgattgtaa ataactcagg atgttcactg
     3001 cccggtcaat gtgttcggct ttggccgtca aatttcctaa ttcaatatct gaggcggcca
     3061 ggttgagaaa tttcatcgct ccatcaaaga gcatgacgac ctgccgcaac gggtttgact
     3121 ccagattggt catttgctgg taagctgaaa ttgccttttt cccaccgtac atagcgttct
     3181 ccttccctca aaacaggtgc tgatttcttt ttcttgcagt ggttatcggc gggattccca
     3241 caaacttgag gcagaattcc gaaaaaatga tcggcccggt cactcattga gagatagagg
     3301 taccgttcat cggtcatttt cgcggatcca tccaaaaaat gagcctttca tcaggtgaga
     3361 tatgcgatac cgctcaaaat cgaatagggt tgtcgcatcg aaaacgactt taactgcctg
     3421 aggtgatact gatgatgtgt tgtacccttc acgtacccac acgattgaga ggtcttttga
     3481 ccattctgtt tttcggctgt ctgttcgttg ccagtccgct caccgtagcg gctcagcaag
     3541 ctgcggcgat accgccctca aagctggcgg caacggttga ggaggatttt cgcgctggaa
     3601 tgagcggcaa tcaggatgcc tttgaccggg cgatgaaatc aaccgaagaa ctgctggcgg
     3661 gtaaccctgc ccatgccgaa gcgctggtct ggcacggctc cgggctggtc tcaaaaagtg
     3721 gaaaagcctt tatggcgggt gattttcagc aaggtagcta ctttatggac aaagggctgg
     3781 cggaaatgac caaagcggtc gaactggaac cagaaaatgt ccatgtccga tttactcggg
     3841 gctcgaccct gctggtattg agcaaggttt ttccaatccc agacactgcc caggactgcc
     3901 tc
//
LOCUS       DAOQDO010000282         3900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16513_length_3900_cov_4.421326, whole genome shotgun sequence.
ACCESSION   DAOQDO010000282 DAOQDO010000000
VERSION     DAOQDO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16513_length_3900_cov_4.421326"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKZ53_05810"
     CDS             complement(<1..608)
                     /locus_tag="PKZ53_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNJ39985.1"
                     /translation="MRLDRFTLRAQEAIQDAVAMAEQAQHPQVEPEHLLVAMLEQNEG
                     IARSILEKIGASVPSIVQDAREVIGKLPKVSGAQRYFSPKVSAIFTAAQKEADQFQDA
                     YLSTEHLLLAIAGDKDSTAGRILRQRGVNREDLLKVLQQLRGGERITDQDPESKYESL
                     KKYGRDLTELARIGKLDPVIGRDDEIRRVVQVLSRRTKNNPVL"
     gene            885..2471
                     /gene="purH"
                     /locus_tag="PKZ53_05815"
     CDS             885..2471
                     /gene="purH"
                     /locus_tag="PKZ53_05815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNJ39986.1"
                     /translation="MTTHLIKRALLSVSDKTGLVEFARGLQSLGIELISTGGTAESLQ
                     KEGLPVKDVSSITGFPEILDGRVKTLHPNVHGGILARRDLATHCAQLEEHRIEPIDLV
                     VVNLYPFTQTIAQPGVTLEAAIEKIDVGGPTMVRAAAKNFRHVAVVTDPHRYSAILEE
                     LQTQGGALTLATRFALATAAFEHTASYDLAISEFLTNRAQFDAGSNQFQVSETELFPA
                     HLSFQLERRQALRYGENPHQQAALYQLTTTDRSGIAFADQLQGKELSFNNLIDLDAAW
                     NLVCEFDHPACAIIKHTNPCGVGVASVPLEAYRQALATDPVSAFGGIIAFNSQVDAGT
                     ATEVTSMFVEAVIAPDFDEASLEIFAAKKNVRVMRARPGHSATGWNVRSVSGGILVQT
                     QDTLQVTPDQLRVVTERQPTETELQDLLFAWTVCKHVKSNAIVYARNLQLVGVGAGQM
                     SRVDSVKIGAMKAKLPIEGGVLASDAFFPFRDGLDAAAEHKITAVIQPGGSVRDAEVI
                     AAANEHGLAMVFTGVRHFRH"
     gene            2600..3775
                     /locus_tag="PKZ53_05820"
     CDS             2600..3775
                     /locus_tag="PKZ53_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ39987.1"
                     /translation="MSNEIENPQPPDEPGKNGQSNEPESNNGDAVHADSGEASRSPEE
                     GQPVSAADPSSHTETTRVRCRFCNQLNQLRAGVSLSKDGHIRCGRCRLPLSEETHTKF
                     GTIDPHSYIHPLDSQALRTLQMIPGIDPILKKFLEVTGESILRIIFSANGVRVTERQC
                     PDLNAKLEVVCSTLGVEKPELYLSVTNPLGGGGGLGFNAFTSGVEKPFIVVFSPLIER
                     LDDTEVLAVLAHEVGHIHCNHLLYKVAAELLFMLANTALESAPIPPGLISLITLPVRA
                     ALMTWYQKAELSCDRAALLVTQDSKALVNVMMKLAGGSMTSKVNYEEFINQAREFDKS
                     TQASFIDSFWTSILASGRTHPFPVWRVSEILKWTEDPEGYERVLKLGQPQQTQAASA"
BASE COUNT          922 a         1029 c          981 g          968 t
ORIGIN      
        1 agcaccgggt tgttttttgt ccgtcgtgac aaaacctgca ccacgcgccg gatttcatcg
       61 tcgcgaccaa tcaccgggtc cagctttcca atgcgggcca gttcagtgag gtcgcgcccg
      121 tactttttca gcgattcata ctttgattca ggatcctgat cggtaatccg ctcgccaccg
      181 cgcaattgct gcaacacctt gagcaaatct tcacggttga cgccccgctg acgaagaatt
      241 cgcccggcag tactgtcctt atcaccagca atggccagca acaaatgttc ggtactcaga
      301 taggcatcct gaaattgatc ggcttctttt tgagcggcgg taaaaatggc gctgactttg
      361 ggagaaaaat agcgttgagc accactaact ttcggtaact tgccaatgac ctcacgggca
      421 tcctgcacaa tcgacggaac actggcgccg attttttcca ggattgatct ggcaatgcct
      481 tcgttttgtt caagcatggc cactaacagg tgttcgggtt caacctgagg atgttgtgcc
      541 tgttcggcca tggcgacggc atcctgaatg gcttcctgag cgcgcagtgt aaagcgatct
      601 aatctcataa atatctccaa agtgtcgaaa tcaggttcct gatgtctcgg taggtgtaag
      661 caaaccagtt tagctagtaa atcttagcgt ggcgataata taaatatctg agtgaatcat
      721 tgtcaagttt tattcaccgg cttttgagat caccagatcc agacacgcct gcgaccgggt
      781 gaccttcatt caccagagcc gaaggaagat tgcaggtgtt gcaaagcgtt tggtagagtc
      841 actcaaactt tatctttatt tttccccctc ttgctttctt ttttatgacc acacatctca
      901 tcaaacgtgc actcttaagc gtctctgaca aaaccgggct ggttgaattt gcccgtgggt
      961 tacaatcact tggcattgag ctgatttcga ctggtggaac ggcagaaagc cttcaaaaag
     1021 aaggtcttcc agtcaaagat gtctcaagca taaccggctt tccggagatt ctggatggtc
     1081 gagtcaaaac ccttcaccca aatgtccacg gaggcatcct ggcccgccgt gacctcgcaa
     1141 cccattgtgc gcaactcgaa gagcaccgga tcgaaccaat tgatctggtt gttgtcaatc
     1201 tctatccttt tacccaaacc attgctcaac caggagttac tctggaagca gccatcgaga
     1261 aaattgacgt gggcggaccg accatggttc gggcggcagc gaaaaacttt cgccatgtcg
     1321 cagtagtcac tgatccgcac cgatattcag ccattctgga agaactccag actcagggcg
     1381 gcgcactcac gctggccacc cgatttgcgc tggccacggc ggcctttgaa cacaccgcca
     1441 gctatgatct ggcaatttca gaatttctca ccaatcgggc tcagtttgac gccgggtcca
     1501 accagttcca ggtatcagaa acggaactct ttcccgcaca cctttcgttc cagcttgaac
     1561 gtcgtcaggc gcttcgctac ggagaaaatc cacaccagca ggcagcgctg taccagttga
     1621 ccaccacaga tcgaagtgga atcgcctttg ccgaccagtt acaaggaaaa gaactctcct
     1681 ttaataatct gattgatctc gatgccgcct ggaacctggt ctgtgaattt gatcacccgg
     1741 cctgtgccat catcaaacac accaatccct gcggggtagg cgtggcatca gtgccactcg
     1801 aagcctatcg ccaggccctg gccaccgacc cggtatcagc ttttggggga attattgcct
     1861 ttaattccca ggttgacgca ggaacggcga ccgaagtcac cagcatgttt gtcgaagcgg
     1921 tgattgcacc cgattttgac gaagccagtt tagagatttt tgcggccaag aaaaatgtac
     1981 gagtcatgcg ggcccggcct ggacattcag ccaccgggtg gaacgtgcgc agtgtttcag
     2041 gcgggattct ggtccaaact caagacacgc tccaggtcac acctgaccaa ctccgggtcg
     2101 tcaccgagcg tcagccaacc gaaaccgaac ttcaggacct gttgtttgcc tggaccgtgt
     2161 gtaagcatgt gaaatcaaat gccattgtgt atgcacgcaa cttgcaactg gtcggagttg
     2221 gcgctggcca gatgagccgg gttgattccg tgaaaattgg tgcaatgaag gccaaattgc
     2281 ccatcgaggg cggggtgctg gcctctgacg cctttttccc gttccgtgat ggcctcgatg
     2341 ccgccgctga acataaaatc acagccgtta tccagcccgg cggctctgtc cgcgacgcag
     2401 aagtcattgc ggctgccaac gaacatggtc tggccatggt ctttaccggc gtcaggcatt
     2461 ttagacatta gagctatgtt cttggtgctt cgttcttggt tcctggttct ttttcaaaag
     2521 gttgatcatt ccgcaaccag caataacgat caggcaccag tcaccacact ttatttcatc
     2581 ccaaagtaag gaaccaccta tgtccaacga gattgaaaat ccgcaaccac ctgatgagcc
     2641 tgggaagaac ggtcagtcaa atgagccgga atcaaacaac ggagacgcgg tccatgccga
     2701 ttcgggcgag gcatcacgct cgcccgaaga aggccagccg gtttctgcgg ctgacccgtc
     2761 aagccacact gaaaccaccc gggttcgatg ccggttctgt aaccagttaa accagctccg
     2821 agctggggta tcgctttcaa aagatggtca tattcgctgt ggacggtgtc gccttccact
     2881 cagtgaagag acccatacca aatttgggac tattgaccca cacagttata tccatccact
     2941 ggattcacag gcccttcgga cgctgcaaat gattccagga attgatccaa tcctgaaaaa
     3001 gtttctggaa gtcactggtg aaagtatttt gcggattatt ttctcggcca atggtgtccg
     3061 ggtcactgag cggcaatgcc cggatctgaa tgccaagctg gaggtggtgt gctcaacttt
     3121 gggagttgaa aaaccggaac tgtacctgag cgtcaccaac ccgctcggcg ggggcggcgg
     3181 acttgggttt aatgctttta ccagtggcgt tgaaaaaccg tttattgtcg tgttttcgcc
     3241 attgattgag cggcttgatg ataccgaggt cctggctgtt ctggctcacg aagtcggcca
     3301 tatccactgt aaccacttgc tgtataaggt tgccgctgaa ttgctgttta tgctggccaa
     3361 tacggctctc gaatcagctc cgatcccccc aggattgatc agtttgatta cactcccggt
     3421 tcgagcagcc ttgatgacct ggtatcaaaa agctgaattg agttgtgatc gcgcggcgct
     3481 actggtgacg caagattcaa aagcactggt caatgtcatg atgaagctgg ctggcggaag
     3541 tatgacctca aaagtcaact atgaagaatt catcaaccag gctcgtgaat ttgataaatc
     3601 cacccaagcc agcttcattg acagtttttg gacctcaatc ctggccagtg gacgaacgca
     3661 tcccttcccg gtctggcgtg tttctgaaat cttgaaatgg accgaagatc cagaaggcta
     3721 tgaacgagtc ttgaaacttg gccaacctca gcaaacgcag gccgcttcag cctgaaaaaa
     3781 ctagacttga gaatgaagaa acaattggtg cttggttctt ggtgcttggt tcttggtgct
     3841 tggttcttgg tgcttggtgc ttggtgcttg gttcttggtg cttggttctt ggtgcttggt
//
LOCUS       DAOQDO010000283         3891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16582_length_3891_cov_3.493743, whole genome shotgun sequence.
ACCESSION   DAOQDO010000283 DAOQDO010000000
VERSION     DAOQDO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16582_length_3891_cov_3.493743"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /gene="hrcA"
                     /locus_tag="PKZ53_05825"
     CDS             complement(<1..728)
                     /gene="hrcA"
                     /locus_tag="PKZ53_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNJ39988.1"
                     /translation="MSKKAPEFVFDERTRDILLSIVKLHIATGEAVGSRTLSKNSKQI
                     LSAATIRNIMADLEEAGYLSQPHPSAGRIPTDKGYRYYVDNLIRGARVSRSDEEFIHR
                     SLLTDPELTRENLLERTSHLLSQISHHVGIVLSPRFSQDIIQHIEFIRLSEGRILVIV
                     VSRTGIVHDCVVRLDEDLSQDELNRTSRYLSENFKNCSLGAIREELLVRMTEEKALYD
                     RLLKNAILLCTQTIDHDAEQADVYV"
     gene            1120..2202
                     /gene="pdxA"
                     /locus_tag="PKZ53_05830"
     CDS             1120..2202
                     /gene="pdxA"
                     /locus_tag="PKZ53_05830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNJ39989.1"
                     /translation="MTQEFSASTPSTWKPRIGITIGDPSGIGPEIALKACADPEVRAV
                     ADLILVGDANHLRQVARHLDLPFEMDVWPFDELVARFSEASIPTQSDRPLVCTIDNLF
                     EPLTWGEISPTYGKASGEYIEAAVKLYQAGIIEGLTTCPINKRSLLLGGYSFTGHTEL
                     LAHLTGTRTFALGFVSAVLRVVLLTNHLPLSRALTYITKERIERMLTLTHRELIRWGI
                     RRPRIAVAAVNPHGAEGGLFGLEEASEMVPAIETCLTENPDMNISGPIPAENVFLRAA
                     RGEFDIVLCSYHDQGMIPIKCLSFGEAVNVTLGLPFIRTSTNHGTAFDIAGRGLADSQ
                     SLIEAIKLAAELVRRGQEFSDDLVEI"
     gene            2245..2508
                     /locus_tag="PKZ53_05835"
     CDS             2245..2508
                     /locus_tag="PKZ53_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNJ39990.1"
                     /translation="MILARTWGTVVATRKDSRLQGHKLLVVKPINLEGQEENSFLIAI
                     DTVGAGARETVLVVSGSSARLAHGLKECPVDAAIVGIVDTVTV"
     gene            complement(2525..2857)
                     /locus_tag="PKZ53_05840"
     CDS             complement(2525..2857)
                     /locus_tag="PKZ53_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39991.1"
                     /translation="MGSEPGLLTKMDWWIEKLTPDHHSQAGLFCLLWLGAALFRSWPM
                     LLASLGFIGFALCFGHPGTFQRPLRLAENSSNRWVGILIVIWGLGSLVSYVLVAVWNF
                     WQLVASKL"
     gene            complement(2957..>3891)
                     /locus_tag="PKZ53_05845"
     CDS             complement(2957..>3891)
                     /locus_tag="PKZ53_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNJ39992.1"
                     /translation="LNEGFATYSEVLFFEKNFDLFAGDLMVDYYDDGRAGGALRGTVY
                     AEDPGDPFDDTNAIYTKGAWVLHMLRGMMGDDVFFATLRDYRQRFAFGNATTADFQKV
                     CEDHYGAPLTDFFTEWVYSERRPIYSYVTQTRTNADGTYTVLVRLDQRQTHALVGQKG
                     PSTYVVPVQLSVVMEGGEKQTFQVLNRFRSQVFALKVPAKPRSVSFDGDNFLLKKIVQ
                     PATTSIGKALAMEASASVVTGKAPLTVELTGTAGGAVLPASSMTWSTERQVIGQGSQL
                     SYTFDRPGVYQVTLTGVNKKTASTSAPIQIVVTE"
BASE COUNT          961 a         1053 c          974 g          903 t
ORIGIN      
        1 acatagacat ctgcctgttc agcatcatgg tcaatggtct gggtacacag caaaatcgca
       61 ttttttaaca gccggtcata gagggctttt tcttcggtca tgcggaccag aagttcttcg
      121 cggatggccc ccagactgca gtttttgaaa ttttctgaca gatagcgcga cgtgcgattg
      181 agttcatcct gtgataaatc ttcatcgagt cgcaccacac aatcgtggac aatcccggtg
      241 cgcgaaacaa caatgaccag gatccgccct tctgacaaac gaataaactc aatgtgctga
      301 atgatatcct gcgagaacct gggtgacagg acaatcccga catggtgcga gatttgcgac
      361 agcaggtggg atgtccgttc aaggaggttt tcacgggtca actctgggtc tgtcaaaaga
      421 gaccggtgga taaactcctc atctgaccgg gaaacccggg ccccccggat caggttatca
      481 acataatagc gatagccttt gtcagttggg atccgcccgg ctgatggatg tggctgagat
      541 aaataccccg cctcttctaa atcagccata atgttgcgga tggtggcagc actcaggatc
      601 tgtttggaat tttttgaaag cgtgcgggaa ccaaccgcct caccagtggc aatatgaagt
      661 ttcacaatcg ataacaaaat gtcacgagtg cgttcatcaa aaacaaattc aggcgctttc
      721 ttgctcatac ggaactggat gcggaggagg ttagtgtggg gattaggcca aaagtaccat
      781 ttcgcaaaac cgactgtcaa ggtgcgttgc gtggcacaaa ggggcgaatc cccttatctt
      841 ttcagtgttt agcttcaata gcggcaattg gcgactgggg atgtttctgg caggggctgc
      901 acccgatatt gatttttgcc caacatttat gatacttccc gaacgcctga accggcgctg
      961 tccccaaaac cctggaactc gcgtgaaggg caagatgctg tagtgagcca aaagtatctc
     1021 gactggctca tgtgcgttct gtttttggca tctcggcctc atctttcagc ccggccccac
     1081 aacccaagaa cgacttctgg tacgttagga tatcaaacct tgacacaaga gttttccgcc
     1141 tctaccccat ccacctggaa accccgaatc ggaatcacca tcggagaccc ctccggaatt
     1201 ggcccagaaa ttgccctgaa agcctgtgcc gacccagagg tacgtgccgt ggctgatttg
     1261 atcctggtcg gagacgccaa tcacctgcgc caggtcgccc gccaccttga tcttccattt
     1321 gagatggatg tctggccctt tgatgaactg gtcgcccggt tttctgaagc ctcaattcca
     1381 acccaaagtg accgcccgct ggtgtgcacc attgataatc tgtttgaacc tctcacctgg
     1441 ggtgaaatct ctccgaccta tggaaaagcc tcaggcgaat acatcgaagc agcggtcaaa
     1501 ctctatcagg ctggcattat tgaaggatta acgacctgtc caattaacaa acggtcgctt
     1561 ctgcttggtg gctatagctt taccggacac accgaactcc ttgcccattt gaccggaacc
     1621 cgcacctttg ccctgggctt tgtctcagca gttctccggg ttgtgctcct gaccaatcat
     1681 ctccccctga gccgggcatt gacctacatt accaaagaac gcattgaacg gatgctgacg
     1741 ctcacccatc gtgaactgat ccggtggggc attcggcggc cacgcattgc cgttgcggcg
     1801 gtcaacccac acggggccga aggagggtta tttggccttg aagaagcatc ggaaatggtt
     1861 cccgcgattg aaacctgcct gactgaaaat cccgatatga atatcagcgg acccattccg
     1921 gcagagaacg tctttttgcg ggcggcgagg ggtgaatttg acattgtcct gtgctcctat
     1981 cacgatcaag gcatgattcc gatcaaatgc ctgtcgtttg gcgaagccgt caatgtgacg
     2041 cttggcctcc ctttcatcag aacctccacc aaccacggca cggcgtttga cattgccgga
     2101 cgcgggctgg ctgactctca aagcctgatc gaagccatca aactggccgc cgaactggtc
     2161 cgtcgtggac aggaattttc agatgatctg gtcgagattt gattgggtcg aaagtcagtc
     2221 gtcacaacct ttgagggaat tatcatgatt ttggctcgaa cctggggaac ggtggttgcc
     2281 acccgtaaag attccagact acaaggtcat aaattgctgg tggtcaaacc aattaacctg
     2341 gaaggccagg aagaaaatag ctttttaatc gccattgata ccgttggcgc cggggctcgt
     2401 gaaaccgtgc tggttgtttc cgggagctca gcccgacttg cccacggact caaagagtgc
     2461 cccgttgatg cagccattgt ggggattgtt gatacggtaa ccgtctaatc aatctaaaat
     2521 tgagctaaag ttttgatgca acaagttgcc agaaattcca gacggcaacc aacacatagc
     2581 tcaccagtga acccagcccc cagatcacaa tcaaaatccc aacccaccgg tttgacgaat
     2641 tttctgcgag acgaagggga cgttgaaacg tacctgggtg accaaaacag agggcaaatc
     2701 caataaaccc caaactggcc agaagcatgg gccagctccg aaacagcgcc gctccaagcc
     2761 aaagcaggca aaagagaccc gcctgcgaat gatgatctgg ggttaacttc tcgatccacc
     2821 aatccatttt tgtcagcagg cctggttcag atcccatcat ggacctcgct tcctgaagag
     2881 acacctgaaa aaaccctcgc cactccaact cggtgcggcg agggtttttc atattcggaa
     2941 ttttggacgt cgtggattat tcagtcacga caatctgaat cggggctgac gttgaagccg
     3001 ttttcttatt gaccccggtc aaggtaacct ggtacacgcc gggccgatca aaggtgtagc
     3061 tcaactggct tccctggcca atcacctggc gttcggttga ccaggtcatc gaactggctg
     3121 gaagcaccgc accgcccgca gttccagtta attcaactgt taagggggct ttcccggtga
     3181 ccacactggc actggcttcc atggccagcg cttttccaat cgaggttgtg gcaggttgga
     3241 caattttctt taagagaaaa ttgtcgccat caaaactcac gctacgtggt ttcgccggta
     3301 ctttaagcgc aaaaacctga gaccgaaacc ggttgagcac ctgaaatgtc tgcttctcgc
     3361 caccttccat caccactgaa agctgaaccg gcaccacgta ggtggacgga cctttttgac
     3421 cgaccagagc atgggtttgc cgctggtcga ggcgaaccag cacagtgtac gtgccatcag
     3481 cattggttct ggtctgcgtc acatagctat aaatcggtcg gcgttcagag tacacccatt
     3541 cggtaaagaa atcggtcaag ggtgctccgt agtggtcttc gcacaccttc tgaaaatcag
     3601 ccgtggtcgc atttccaaaa gcaaaacgct gacggtaatc acgcaaggtc gcgaaaaaga
     3661 cgtcatctcc catcatgccg cgcagcatgt gcaagaccca ggcccctttg gtgtaaatgg
     3721 cattggtatc atcaaatggg tcacctggat cctcggcata gactgttcca cgcagggcgc
     3781 cgccggctcg cccgtcatca tagtagtcaa ccatcaagtc accagcgaac aggtcaaagt
     3841 ttttctcaaa aaatagaact tccgaatagg tggcaaaacc ttcgttgagc c
//
LOCUS       DAOQDO010000284         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16597_length_3889_cov_3.424883, whole genome shotgun sequence.
ACCESSION   DAOQDO010000284 DAOQDO010000000
VERSION     DAOQDO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16597_length_3889_cov_3.424883"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..3062
                     /locus_tag="PKZ53_05850"
     CDS             132..3062
                     /locus_tag="PKZ53_05850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ39993.1"
                     /translation="MASLALPQPQSDRKQITHQTSLLYAAQLTGTGLNFATTFLLARW
                     MGVDNFSLFTFCSVSILTFVSLFFEFGMFSAGARLLAISKDETEENEVLGALLVLGLG
                     LGLAMAIFIGVSAPFIDQIFRVQVRGMLWACLPLVLFIPLQFLLESACQGNNRISSLG
                     LFRIALPGLTFLGAALVFAFFQLTPFGALLATNAGAAAACLLVIAFLRPSFHNLRSHV
                     RAILDETRRFGIDVYLGRVTAIASQRADGLLIPYYIGYAPLGFYTIGKSIADSLIINF
                     SRALAVTRFRSFARSHEVSNGVLVWNFLLLLVAGTALILVAPLLLGRFLPQYVQSADL
                     LVPFVVGSILGGLLQPYNLFLAAHGRGGELRNISMFIGVVYLVALVLVIPWFGLLGAA
                     WVIAAVAGLNFVLHRQHYLNLQRERITTGEIPLRVVVINLSGDTRHARAWAESLTANV
                     ELTLIDKARLREDSKLNWIRRLRQTPCDLFAIYCDRLEWQTLRIPMLLFGMLAGARTC
                     LIVDSAGKELRRSALAVALWEIPHLALEVCGGAFLIGISWALTRYLELECRMAQSRTP
                     SNPLSTLHHSTSSRPESLLFIRTTPTSGAQTGGATSHISGFTQGATALGVKVRFLSND
                     SISGVHPEVTPIKILWPSGFFSAHRVLFELWNNLVFTIRAIDEVKNDPPDYLYQRYSR
                     FNWSGVVLSLSTGIPLLLEYNGSEVWIGRYWDDVQLLWLLEAFEMLNLQAAASVFVVS
                     EVERQNLLERGIPDTKILVNPNGVNPDIFHSNCGGTAVRHQLGLEKQVVVGFVGTFGP
                     WHGVEVIAKAATLLPSDSPLHFVMIGEGALKSTVEEQVQKTGVSHRFTFTGRIGHNRV
                     AAYLDACDILVSPQVQREDGGEFFGSPTKLFEYMAMEKGVIASRVGQIGQILEHEKTA
                     LLIPPGDAQALADACERLARNPQFCRDLGSAARTVVSGNYTWKHNAQRVLDRAASLQA
                     NSQS"
BASE COUNT          828 a         1005 c         1005 g         1051 t
ORIGIN      
        1 ctggttttca gccccaggcc ctgagccctg gttttcagcc ccaggccctg agccctggtt
       61 ttcagcccca ggccctgagc ccagagtttc ttcagcccca aaatttacga ctgaccactt
      121 ttatcttcac aatggcttca cttgctttac ctcaaccgca atctgatcgg aaacaaatca
      181 cccatcaaac cagcctgctc tacgcggctc aactcactgg aactggtctc aactttgcca
      241 ccacattttt gctggctcgc tggatggggg ttgataattt cagtctgttt accttttgtt
      301 cagtctcgat tctcaccttt gtcagcctct tttttgaatt tggaatgttc tcggctggtg
      361 cgcgcctcct ggcaatcagt aaggacgaaa ctgaagaaaa cgaagtcctt ggcgcactgc
      421 ttgttcttgg gctagggctg gggctggcca tggcgatatt tatcggtgtc tcggcaccgt
      481 ttattgatca gatttttcgg gtgcaggtcc ggggaatgct ctgggcctgt ctcccgctgg
      541 ttttgtttat tccgcttcag tttttgcttg aatcagcctg ccagggcaat aaccgcattt
      601 cgtcgttggg attatttcgc attgcactcc ccgggttgac ctttctgggc gccgcgttgg
      661 tgtttgcctt ctttcaactc acgccttttg gagcgcttct ggcaaccaat gcgggtgcgg
      721 cggcagcctg tttgctggtc attgcctttc tccgcccttc gtttcacaac ctgcgctccc
      781 atgtgcgtgc gattttggat gaaacacgcc ggtttgggat tgatgtttac cttggccggg
      841 tcaccgcaat tgcctcacaa agagctgatg ggcttttaat tccttattat attggctatg
      901 ccccgcttgg gttttacacg attggtaaaa gtattgccga ctcgttgatc atcaattttt
      961 cgcgggcact ggccgtgacc agatttcgga gttttgcccg atcacacgaa gtttccaatg
     1021 gcgtactggt ctggaatttt ctattactcc tcgtggctgg caccgccttg atcctggtcg
     1081 ctccattgtt gctaggccgt tttttaccgc aatatgttca aagtgcagat ttgctggttc
     1141 catttgtcgt ggggagcatt ctcggtggcc tgctacagcc ttacaatctc tttctggcag
     1201 cccacggacg tggcggtgaa ctccgcaata tttcgatgtt tatcggtgtc gtttatcttg
     1261 tggctctggt gctggtcatt ccctggtttg ggctgttggg tgccgcctgg gtgattgcgg
     1321 cagtcgccgg actcaacttt gttttgcacc ggcagcacta tctcaacctg caacgcgaac
     1381 gcatcaccac gggcgagatt ccactccgcg tcgtggtgat caacctgtca ggagataccc
     1441 gacacgccag agcctgggca gaatcactca ctgccaatgt tgaactgacc ttaattgata
     1501 aagccaggct tcgtgaagat tccaaactca attggattcg gcggttgcgt caaacaccgt
     1561 gtgatttatt tgcgatttat tgtgaccggc ttgaatggca aacgctacgg attccgatgc
     1621 tcctgtttgg aatgctggcc ggggctcgaa cctgtttgat tgttgattct gccggaaaag
     1681 aattgcgccg ctcagccctg gcggttgccc tgtgggaaat cccacatctg gcgcttgagg
     1741 tgtgtggtgg ggcctttctg attgggatct cgtgggcatt gacccgctat cttgaacttg
     1801 aatgccggat ggctcaatcc cgaacgccct ccaatcctct atccacgctc catcactcaa
     1861 catcatctcg ccccgaatcc ctgcttttta tccgcacaac ccccacgagt ggcgcccaaa
     1921 ctggcggagc aaccagtcac atttcagggt ttacacaagg ggcaaccgca cttggggtca
     1981 aagtgcgatt tctttccaat gacagcattt cgggagttca cccggaggtt actccgataa
     2041 aaattctctg gccttcgggg ttcttttcag cccatcgcgt gttatttgaa ctctggaaca
     2101 acctggtgtt taccatccgg gcgattgacg aagtcaaaaa tgatccgccg gactatctct
     2161 atcaacggta tagccggttt aactggtcag gggttgtgtt atcgctctcg accggcattc
     2221 cattgctgct tgaatacaac ggctctgaag tctggattgg ccgatattgg gatgacgtac
     2281 agttgctgtg gctcctggaa gcttttgaaa tgttgaactt acaggcagca gcctcagtgt
     2341 ttgtcgtctc tgaagtcgaa cgccagaatt tgcttgagcg cggaattcct gataccaaaa
     2401 tcctggtcaa ccccaatggt gtcaatcctg atatttttca ttcaaactgt ggtggaacag
     2461 ccgttcggca tcaactcggg cttgaaaaac aggtcgtggt tgggtttgtc ggaacgtttg
     2521 gcccctggca cggagtcgaa gtcattgcca aagccgcaac ccttttgccg tcagattccc
     2581 cattgcactt tgtgatgatt ggcgaaggcg ccctcaagag cacagttgaa gaacaggtcc
     2641 aaaaaactgg cgtatcgcat cggttcacat ttactggacg tatcggccac aaccgggtcg
     2701 ccgcctatct tgacgcctgt gacattctgg tttctccaca ggttcaacgc gaagacggcg
     2761 gtgaattttt tggctcacca accaagcttt ttgaatatat ggccatggaa aagggagtaa
     2821 ttgccagtcg ggtgggtcag atcggccaga tcctcgaaca tgaaaaaacg gccctcctga
     2881 ttcctccggg agatgcccag gctctggctg acgcctgtga acgcctggct cggaaccctc
     2941 aattttgtcg tgatttaggt agcgctgccc gaacggtagt ttctggaaac tatacctgga
     3001 agcataatgc ccagcgtgtt ttggatcgtg cagcaagtct ccaggcaaac agtcagtcat
     3061 aaaaaaaagc gacatagaaa atcccagatg ggagttcact atgtcgccaa acgatccaaa
     3121 caagaatacg gtgcagagaa aacttgtgac atttattcag aagtacgtct gggtgatcac
     3181 tgaaaaatga gatcaggatc aacgaaaacc agagggcgaa tatgtctcag catattcggt
     3241 tcaattctga attttagata aagcggagct gtcacaatca aagtacagat gagtgagttg
     3301 ccgatttatc cggtctgatg atatagacgc aaccgggaag gctttcttcc gtaattttgg
     3361 tcgaaaattc aacattgttt gcagtatcat atagtcagga gtcagtggtc aggagccagg
     3421 agttgactaa gggactgtaa aaaatcaact tcccggtttc agttggaagc gtggggggcc
     3481 tctccggccc aggtttttgg gtttgcaccg cgaagcgttg cggttcacat agccggtcgg
     3541 ttggccgcgt tgggcctacc accggactca agcgccaccc cctcgtttct ccccgctttg
     3601 cccgcgccca gggggcgcgg gcaaagcggg gagggatggg ggaaatcaaa ccgtttcccg
     3661 gtggtagctc tcaaagccga gcaaccgaca ggctatgtga acggcagcct ttcaggatgc
     3721 tcaatccaaa taccgatccc taaacgaaaa cgggaagttg atttttaacg ctccctaagg
     3781 ttatttgatt gaagcacttg actgtttgtc aacagactcg gccctgaagg gccgagcttg
     3841 agtcgtgtac ccacgacgcc atcctgacgg atggtcggtc gtgggtgga
//
LOCUS       DAOQDO010000285         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16600_length_3889_cov_2.586072, whole genome shotgun sequence.
ACCESSION   DAOQDO010000285 DAOQDO010000000
VERSION     DAOQDO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16600_length_3889_cov_2.586072"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKZ53_05855"
     CDS             complement(<1..260)
                     /locus_tag="PKZ53_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39994.1"
                     /translation="MGKWTFFLRIELGFYVPGGAWSGRVLALWGDLREEEVKRVKRQQ
                     VVEVLEVKVKVVEHQGGVQSVSEMWEGKRGEVPAGVGNGVQYG"
     gene            345..1622
                     /locus_tag="PKZ53_05860"
     CDS             345..1622
                     /locus_tag="PKZ53_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ39995.1"
                     /translation="MYNLSTGIDFSVSFSPLDLTMAREELGLSMRELAEKLGLSVAAI
                     SRWESSNREMTYSEILRWALIRLKEIRNQEEWEERINERGGTQGHTEADGIEGGNDGK
                     NFGSVIANISPMGEREGENPDSHNVASGVGGYRSPCPNNGNLYTRADDSQKTGASEKE
                     MSNQCCVVAVFNQAGGVGKTTLTRDVGYELSSRGFKVLLVDADPQGTLGTFFGFSPGE
                     TPREQLFWSNICERNNQKPPSRVRTEFGLDVGLANRQLLTDEMFLMQQRDMARVSAVM
                     GRLRKEYDWILIDCPPKMAEISYQILLAVDGLLVPVQTEPKAVVSFVETQLEIVDANG
                     RRANMGLPPLRVYGVIPTIYNPRLLLHQHEHKALRDEVCPEFGYQLYPAIPRFIAVSE
                     AGLLKRPLKDYDDRCPVNAVVAEITDRLLKEAR"
     gene            1623..2477
                     /locus_tag="PKZ53_05865"
     CDS             1623..2477
                     /locus_tag="PKZ53_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ39996.1"
                     /translation="MSRKGSLGKASSLSPTRLKGVSLYVPEVAQPAQTNETIKRVPVA
                     DLHPNPHQPRSFINEKALADLTASLSLHGLLQPVILREEAVGYTIVAGHRRVEAARRL
                     GWVEIESVVRRVEDQELLQLAMVENFQREDLHAIDRLRALTRYAQLHPTQQEAARKIG
                     MDSTTLSHWLRLNELGEAVLEACVTIPELSFGNLRRLLNLSPNHRLRAVQKLAAGEGQ
                     PQTEAIKPKSSNGSRSFFRFVYPIASSKQKLLIEIKPKGSAKSKEFTVEEIRSALQAA
                     LADLDTKK"
     gene            complement(2655..3143)
                     /locus_tag="PKZ53_05870"
     CDS             complement(2655..3143)
                     /locus_tag="PKZ53_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39997.1"
                     /translation="MGHCLQAFLGSEALLGDIVQIQSTAIIIPLLQGLAMIPVTEALY
                     DVLTTPEERGSFDPRFLFLSPKLNHFGQTHSHPGPLAYIETDYFGGVGSQGAIVWSKG
                     KIIFGPAVTGNRRDLQVDQLPAGAINCALKMLGVEKRHWVDEFEALGLRTYRAWTNDS
                     TL"
     gene            complement(3171..3749)
                     /locus_tag="PKZ53_05875"
     CDS             complement(3171..3749)
                     /locus_tag="PKZ53_05875"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNJ39998.1"
                     /translation="MTRKLVIAIAGFFLMMGLSCLSAMAQNVKEPSTGVSFLSKGGNY
                     GHTFEVTHLGVRSKLGFKVYAAAAYYETGKYDKAADAKSQMLKDGPAKLIIMHFVRDV
                     EAAKIREQYEESLRKNTPGYDSLKGVVDPFLNSLVDMKSGDRMELCWGPGGAVTLAVK
                     GQVKNRFTNKSLATGLWSIWFGNSPILTWKAQ"
BASE COUNT         1009 a          931 c          986 g          963 t
ORIGIN      
        1 ccatactgca ccccatttcc tactcctgct ggaacttccc cacgctttcc ttcccacatt
       61 tcggacacgg attgtactcc cccttgatgt tccaccactt tcacttttac ttccagcacc
      121 tccaccacct gttggcgttt gactctcttg acttcctctt ctctcaaatc gccccacagt
      181 gccagcactc ttccactcca ggcaccacca gggacataga acccaagttc gatacgtaga
      241 aaaaaagtcc attttcccat ctgttaccaa attagaattg acatttttat aaagttcagt
      301 agtctcggtg gcgttaccaa attataagcc tggacaggta cggcatgtac aatctctcaa
      361 caggtataga tttctctgtt tcgttttcac cgcttgattt gacaatggca cgcgaagagc
      421 ttggtctcag catgagagaa ctggcagaaa agcttggtct gtctgtcgca gctatttctc
      481 gatgggagag ttccaaccgg gaaatgactt attcagagat attgagatgg gcattaattc
      541 gtctcaaaga aatccgtaat caagaagagt gggaggagcg aatcaatgag cggggaggaa
      601 ctcaaggtca tacggaagcg gatgggattg aaggaggcaa tgatggcaaa aattttgggt
      661 cagtcattgc aaacatatcg ccgatgggag agcgggaagg cgaaaatccc gattcccaca
      721 atgttgcgtc tggcgttgga ggctatcgaa gcccatgtcc aaacaacggg aacctttaca
      781 ccagagcgga cgattctcaa aaaacggggg catccgagaa agaaatgagt aaccagtgtt
      841 gtgtcgttgc tgtttttaat caggccggtg gggtgggaaa aacaacctta acccgagatg
      901 tcgggtacga gttaagtagc cgaggcttta aggtgttgct cgtagacgca gacccacaag
      961 gaacgttggg gacattcttt ggattctctc ctggggagac tccgcgagaa cagctttttt
     1021 ggtcaaatat ttgtgaacga aataatcaaa agccgccatc ccgagttcgg acggagtttg
     1081 ggttggatgt tggcctggcc aatcgacagc tcctcacaga tgaaatgttt ctcatgcagc
     1141 agcgagatat ggctcgggtg tcggcggtga tggggcgatt acgaaaggaa tatgattgga
     1201 ttttgattga ttgtcccccg aaaatggctg aaataagcta tcaaatattg ctggcggttg
     1261 atggactttt agtgccggtg caaaccgaac ccaaggccgt cgttagtttt gttgaaacac
     1321 agttggaaat tgtggatgcc aatgggcgtc gggcaaacat ggggttgccc ccccttcggg
     1381 tgtatggagt gattcccacc atctacaacc caaggctctt gctacatcag cacgagcaca
     1441 aagccttacg agacgaagtt tgccctgaat tcgggtacca gttgtacccg gcgatcccgc
     1501 gctttattgc ggtgtctgaa gccgggctct taaaacgtcc tttaaaagat tacgacgaca
     1561 ggtgtccagt aaatgcggtc gtcgcagaaa tcacagaccg cttactgaag gaggcccgat
     1621 aaatgagtcg aaagggaagt ttgggaaagg ccagtagtct gtcaccgact cgattaaagg
     1681 gagttagttt gtatgtacca gaagtggccc aaccggccca aacaaatgag acgatcaagc
     1741 gggtccccgt ggctgatctg cacccgaatc cccatcaacc ccggtccttt atcaacgaaa
     1801 aggctttggc ggatttgacg gcgagtttgt cgttgcatgg gctattgcaa ccagtcattc
     1861 ttcgcgaaga agcagttgga tacaccatag tagctggcca tcggagagtt gaggctgccc
     1921 gacggttggg gtgggtagag attgaaagtg tcgtacggag ggtggaagac caggaattgc
     1981 ttcaattagc aatggtcgaa aatttccaac gagaagacct gcatgcgatt gatcggctcc
     2041 gagcattgac gaggtatgcc caacttcatc ccacgcagca ggaagcggcg agaaaaattg
     2101 gcatggattc gacaaccctg tcgcattggt tacgacttaa cgagttgggg gaggccgtcc
     2161 ttgaagcctg cgtaaccata cctgaactat catttgggaa tcttcgtcgt ttgcttaacc
     2221 tgtcaccaaa tcatcgactg cgggcggtac agaagctggc tgcgggtgaa ggtcaacctc
     2281 aaacggaagc tataaaaccc aaatcttcca atggttcccg gtcttttttt cgatttgttt
     2341 atccgattgc ttcctcaaag cagaaattgc tgattgaaat taaaccgaaa ggaagcgcga
     2401 aatcgaagga attcacggtc gaggaaattc gatccgcgct gcaagcggca ctggccgatc
     2461 ttgatacaaa aaagtaaaag ttggacgcgt ccaaggtttg caaataatag aaaatcaaag
     2521 atttacctgg acttttaaga agtgatgaag gctgatttcg agacctaact tcgtggatat
     2581 ctgagatatt ttgaggattt ctaagagact gtttggcaat tccagtggaa tttcattggt
     2641 tttgcttctt cagactacag agtggaatca ttcgtccatg ctcggtatgt tcgcaggccc
     2701 agcgcctcaa attcatcaac ccagtgcctt ttttccacgc ccaacatctt gagcgcacag
     2761 ttgatggcac ctgctggcaa ctgatcaacc tgcagatcac ggcgatttcc cgtcactgcc
     2821 gggccgaaaa taatttttcc tttcgaccag acaatggccc cctgactgcc tacgcctcca
     2881 aaataatcgg tttcaatgta ggcaagcggc cctgggtgtg agtgggtctg gccaaaatgg
     2941 tttaatttcg ggctcaaaaa aagaaagcgt gggtcaaacg acccacgctc ttcaggagtg
     3001 gtaagcacat catacagggc ttcggtcacc ggaatcatcg ccaggccctg caacaacggg
     3061 ataatgatgg cggttgattg gatctgaaca atgtcaccaa gcaaggcttc tgatcccaaa
     3121 aatgcctgca ggcaatgtcc catggtgact atccctataa ctgaccggct ttattgcgcc
     3181 ttccaggtga gaatcgggga atttccaaac cagattgacc agagtccggt cgccagcgac
     3241 ttgttggtaa accgattttt gacctgacct ttgaccgcca gggtcacggc gccgcctggg
     3301 ccccagcata actccatccg gtcccctgac ttcatatcaa ccagggaatt caaaaatggg
     3361 tccacgacgc ctttcaacga atcataacca ggggtgttct tgcgcagact ctcttcatat
     3421 tgctcgcgga ttttggcggc ttcgacatca cgcacaaagt gcataatgat gagtttggcc
     3481 gggccgtctt tgagcatctg tgattttgca tcggctgctt tatcgtattt cccagtctcg
     3541 taataggccg ccgccgcata gactttgaat ccgagtttgc tccgaactcc aaggtgggtg
     3601 acttcaaatg tatgcccata attgccgccc ttggagagaa atgagacgcc agttgacggc
     3661 tctttgacgt tttgtgccat ggccgagaga cagctcaatc ccatcatcag aaagaaacca
     3721 gcaatggcaa taaccagttt tcgagtcatg agtgtttcct cacaaaaatg aaagtgattt
     3781 tttttcaagc gaatgttgaa agaagaattg gcgggacaac gggcgtgatt gtagcgcatt
     3841 tggaaccagg cgactatcac caattctctt tggcctccgg gttccgggt
//
LOCUS       DAOQDO010000286         3879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16672_length_3879_cov_3.560669, whole genome shotgun sequence.
ACCESSION   DAOQDO010000286 DAOQDO010000000
VERSION     DAOQDO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16672_length_3879_cov_3.560669"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKZ53_05880"
     CDS             complement(<1..237)
                     /locus_tag="PKZ53_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ39999.1"
                     /translation="MHLGPSVIFLVLTALGMCFCTPVQAQAHSPTLEQQAAQFRAYRE
                     ALRKDTHRQKIELRHWNGPVHKLMSELGDRLEQDH"
     gene            complement(257..1141)
                     /locus_tag="PKZ53_05885"
     CDS             complement(257..1141)
                     /locus_tag="PKZ53_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40000.1"
                     /translation="MNFYLSLRLVRFIGAASLLLTLLSGTGLAKTPVRVSTEKQKSEE
                     IISKHLLSIGPADARMSIKTRVLEGLAHFRPQVAGQFDAQGTSIFASEGTKSVLGMKF
                     TVAAYQRELIAFDGVQVTSAYFKPGDRSVLSDYLLPKKVIFQHGLFGGALSTGWALLH
                     FTPEKADIEYGGLKKLDDTEAYLLRYKPKGSSDVTIQLYFEKSTFHHIRTDYEQIVSA
                     QMGETPEKSAQQITERYRVSEEFDDFWNDQGLVLPHYYKLIYSINGGRKFVWHLEFQK
                     MTNNQVIDPKSFVIFTNK"
     gene            complement(1428..2285)
                     /locus_tag="PKZ53_05890"
     CDS             complement(1428..2285)
                     /locus_tag="PKZ53_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HNJ40001.1"
                     /translation="MIPKNHSVYCFSSLQSPVCEVAPGTVLTFETSDEQYERLSRGES
                     VQAVRKLTNPVTGPVAIQGALKGDAVCVEVLDIQITRAWAVWVPNLGSLGHKTQTVQV
                     KEIPIQNNTLQLSPRLRVDLDPMIGCIGVAPKKGIGSTVAPAGRWGGNMDLRELRIGT
                     RLWLPVFHPGGLLSIGDLHAAMGTGEPTCIAIETSGQVTVRIDLIPKAGIESPRLFVP
                     GATLCVGIGQSIEQAQLSAIDQAYQILTEKFGLSDFEAYAYASAKLELRFGGPASPIA
                     LAVIPDPVF"
     gene            2641..>3879
                     /locus_tag="PKZ53_05895"
     CDS             2641..>3879
                     /locus_tag="PKZ53_05895"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ40002.1"
                     /translation="MNSNRRPHRFLASCLFFVGLILIFGIGSQQVVRETEARSQRIPG
                     IQGSAPFASNPWLTNRTMFPRLGNPLRQTPSMAATKVDALQTDVDGDLAADPGDRLRY
                     TVVITNSGGGNATGVNFTDTIDSNTTLVAGSVKVSPLAIADSYNAVGNTTLTINTVSG
                     VRANDYDVDGVTPNGSLVVTAGTFATTQGGSVTIAADGSFTYEPQVGDQNVTDTFTYT
                     LTDGDGLTDTGTVTINLGQRVWYVDSTASAGGNGTFASRFDSLADVTGATGPDTASDI
                     IYIFERTGDYDGNMTLLNNQQLIGSGVALTVNTITVVAAGSNTTLVTTAASTNSITLG
                     SGNTVRGFTIGNTTGAGINGNGIGTFSADTLTINGTGQILNLNNGTINATSGITLSTT
                     STTSVAVVLTSMAGSVTAGGS"
BASE COUNT          970 a          934 c         1027 g          948 t
ORIGIN      
        1 atgatcttgc tccaaacggt ctccgagttc agacatcagt ttgtgaacgg ggccgttcca
       61 gtgccgaagt tcaatttttt gccgatgggt atctttcctc aatgcttcgc gataagcccg
      121 aaactgggcc gcttgttgct caagcgtggg tgagtgagct tgagcctgga caggagtgca
      181 aaaacacatt cccaaagcag taaggaccag aaaaataacg cttggaccca ggtgcataat
      241 tcgaaatcac cgctggttat ttattagtga aaatcacaaa tgatttcggg tcaatgactt
      301 gattgttggt catcttttga aactcaaggt gccatacaaa ttttctccca ccattgatgc
      361 tataaatcag cttatagtag tggggtaaaa caagcccttg atcattccaa aaatcgtcaa
      421 attcttcgct cactcgatag cgttcagtga tttgctgggc tgatttttca ggtgtttcac
      481 ccatttgggc tgaaacgatc tgctcataat cggttcggat atggtgaaat gtacttttct
      541 caaaatacaa ctgaatggtg acatcagagc ttccttttgg cttatagcgc aacagatagg
      601 cttctgtatc gtccagtttc tttaatccac catattcaat atcagctttc tccggggtga
      661 aatgtaacag cgcccatccg gttgagaggg cgcctccaaa caatccatgt tggaaaatta
      721 cttttttggg caaaagatag tcgctcaaaa cagaccgatc accaggttta aagtaggctg
      781 aagtgacctg aacgccatca aaggcaatga gttctcgttg gtatgcggca actgtgaact
      841 tcattcccag gacacttttg gttccctcgg atgcaaaaat agacgtccct tgagcgtcaa
      901 attgtccggc tacctgaggg cgaaaatggg caagtccttc caggacacgg gttttgatac
      961 tcatccgggc atcggctggt ccaattgaaa gcagatgttt ggaaataatt tcttctgatt
     1021 tttgtttttc tgtactgact cgaactggtg ttttggccag acctgtgccg gaaagaagcg
     1081 tgagaagcag gcttgccgct ccaataaaac gtaccaatcg caggcttaaa taaaaattca
     1141 tacaaatcat caagcgccct catcgttgaa ggcactcttt ctcctgaaaa tgttgtgcat
     1201 cgtgcacttg ctgacacctg taagaaccca aaatcagcga accagcgtct ctctgttcag
     1261 tctgttctca tcagccgctc tgtgaggaca gagcgtttaa accgcttcac ggaaaatgat
     1321 ctgtcacatc acaatgggtg tttgagacat tgatgattga gatgaacagg tgacgggtca
     1381 gagtgtacct tagaattgaa ctttaaggtg tgttcttttt tcgattttca gaaaactgga
     1441 tcgggtatca ccgcgagcgc aatcggcgag gcaggacctc caaaccggag ttcgagcttt
     1501 gccgaggcat aggcgtaggc ttcaaagtcg ctcagtccaa acttttccgt caaaatctga
     1561 tatgcctgat caatggccga aagttgagcc tgttcaatgc tttggccaat gccgacgcac
     1621 agggttgctc ctgggacaaa caaccgtggc gattcaatcc cggctttggg aatcagatca
     1681 attcggactg taacctgacc gctggtttcg atggcaatac aggttggttc acccgtgccc
     1741 atcgccgcgt gcaggtcccc aatcgagagc aacccaccag gatgaaacac tggcagccag
     1801 agacgtgtcc cgattcgcaa ttcccggaga tccatattcc cgccccaccg tcccgccggg
     1861 gcaacggttg acccaattcc ttttttgggt gccacaccaa tacagccaat catcggatca
     1921 agatctaccc tcagtcgtgg gctcaattgt aaggtattgt tctggatggg gatttctttc
     1981 acctgaacag tttgggtttt gtggccaaga ctgccgaggt tgggtaccca caccgcccag
     2041 gcccgagtga tctggatatc cagcacttcc acacaaactg catcaccttt cagagcgccc
     2101 tggatcgcca ccggaccggt caccgggttg gtcaatttcc gaactgcctg aaccgattca
     2161 ccacggctga ggcgttcata ttgctcgtcg gaagtttcaa atgtcaggac tgtccctggt
     2221 gcgacttcac acactggcga ctgaagggaa gaaaagcaat aaaccgagtg gttcttggga
     2281 atcatagtgg ccataagtta ttgaaatatt tgtgtttttc tgagattcga aaccctggat
     2341 cacatagtat ggatattgtt tcaggatgtt aagcagaaat aaaggatgtt cctcactgga
     2401 gaaagatttg cctttacttg acttttggca gccaatccat cacaagtagc gccacatcaa
     2461 tcacttatta tcctcttccg tttttacaac ccactcattg atcgctggtt caaggagatt
     2521 tggtaattca gggtatttca ggcacaaaac gtcttgtgtg atgtaccgca agcagttgtt
     2581 cccattgcca atgaacactc cccaccgcca aactgcctga agccggttcg caggtgaact
     2641 atgaattcaa accgccgccc ccatcggttt ctcgcttcgt gtctcttttt cgtcggactg
     2701 attctgatat ttggaattgg aagccagcag gtggttcggg aaaccgaagc tcgcagtcag
     2761 agaattcctg gaatccaggg aagtgctcca ttcgcgagca atccatggct aacaaaccga
     2821 acgatgtttc cacggttggg aaatccgttg cggcaaacgc cgagtatggc ggcgaccaaa
     2881 gtggatgcgt tacagacaga tgtggatgga gatttagcag ccgacccagg tgaccggttg
     2941 cggtacacgg tggtgatcac caacagtggt ggcggaaatg cgacgggagt gaatttcacc
     3001 gacacgattg attccaacac gacgctggtg gccgggtcgg tgaaggtgtc gccgctggca
     3061 attgccgata gttacaatgc agtggggaat accacgctga cgatcaacac ggtgagtggt
     3121 gtccgggcca acgattatga cgtggacgga gtaaccccga acggatcgct cgtggtgact
     3181 gccgggacat ttgcgacgac gcagggtggg agtgtgacga ttgcggcaga tgggtcattc
     3241 acctacgaac cgcaggttgg cgaccagaat gtgaccgaca cgtttaccta taccttgacg
     3301 gatggcgacg ggttgacaga taccggaacg gtgacaatca acctcggtca gcgggtgtgg
     3361 tatgtggatt cgacagcgag tgcgggaggg aatggaacat ttgcgtcgcg gtttgattcg
     3421 ctggcggatg tgaccggggc gacggggccg gacacagcga gcgatatcat ctatatcttt
     3481 gagcggacag gggattacga cgggaacatg acgctgctca ataaccagca attgattggg
     3541 agcggtgtgg cgttgacggt caacacgatc accgtggtgg cggcggggag caacacgacg
     3601 ctggtgacga cggcagccag taccaattca atcacgctcg gaagcggcaa tacggtgcgg
     3661 ggattcacga ttgggaacac caccggcgcg ggcatcaatg ggaatggaat tgggacgttt
     3721 tctgccgaca cgctgacgat caacggaacc gggcaaatcc tgaatttgaa caacgggaca
     3781 atcaatgcca catcaggcat cacgctttca accacgagca cgacgtcggt ggcggtggtg
     3841 ttgacgagta tggcggggag tgtgacggcg ggcggtagt
//
LOCUS       DAOQDO010000287         3879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16676_length_3879_cov_2.697699, whole genome shotgun sequence.
ACCESSION   DAOQDO010000287 DAOQDO010000000
VERSION     DAOQDO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16676_length_3879_cov_2.697699"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKZ53_05900"
     CDS             <1..969
                     /locus_tag="PKZ53_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HNJ40003.1"
                     /translation="VVTGKSWSEFVTERILTPLGMTSTTTSVTAFKPGDNVAAPHNEL
                     DGMMRVIRYSNVDSGAAAAGLNSCVADIAQWLRLQLGRGRYQGKVIFSAKNSREMWTA
                     HTLQPISELAEKLNPTQHFSAYGLGWSLNDYQGRKVISHGGGLDGMISRTAMIPEENL
                     GLVVLTNSETALANVVSNKIFDVFLGAPQRDWSAEFLELTRRRKEAEKLEAQQIEASR
                     IPNTQPSLPLAKYAGKYSGPLYGDATITEENGKLVLRLIPAPNFVGDLEHWHLDTFRL
                     KWRPSVSYPFPKGFVTFTINASGKPIELKIDCPNPDFDFGELELKR"
     gene            complement(1154..1420)
                     /locus_tag="PKZ53_05905"
     CDS             complement(1154..1420)
                     /locus_tag="PKZ53_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNJ40004.1"
                     /translation="MFRKLTLAAVATLALSFSAPAFAQDQTKPEPKPEKEEVVMCPVM
                     KEEVLDTASATFSDYKGKRYYFCCPGCKPKFDKNPEKYVKPETK"
     gene            complement(1497..2570)
                     /locus_tag="PKZ53_05910"
     CDS             complement(1497..2570)
                     /locus_tag="PKZ53_05910"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNJ40005.1"
                     /translation="MKLFQHLQEFGHEQLVFAQDSNIGYKAIIAIHSTALGPAVGGTR
                     FWNYESEEDAIVDALRLARGMTYKNALAGLQLGGGKSVLIGDNRRTDREVLFRAHGRL
                     IDGLGGRYITAEDVGTSPADMELIHQETRHVAGLASRNGDPSPWTARGVFRGIQAAAN
                     YRWGSDDLTGRRIALQGLGNVGTHLARQLHQAGAKLVVTDVSQNRVKRMMAELDVPAV
                     DPADIFSVEADIFVPCALGAVINPETIPQLKVEIVAGAANNQLLDDQSGDELEDRGIL
                     YAPDYVVNAGGILSGGVDLVGWTPDFARSKVEAIYQTVREVFEYAERENIATYKAADQ
                     LAEQRIALAKTTPPKKARSVPGN"
     gene            complement(2918..3757)
                     /locus_tag="PKZ53_05915"
     CDS             complement(2918..3757)
                     /locus_tag="PKZ53_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ40006.1"
                     /translation="MSKKKPPVRLPKQLMTNLAYQSVASQSGDVLLHVETSQIRPNPH
                     QPRKIFDPETIDELAASIREHGILEPLIGQEASDGTYTLIAGERRWRAAQALNLSRVP
                     MVLRGGNQTTDTGLLALVENLQRADLHPVEEVQALARIVEQCATQEEAARLVGFHRST
                     LARLIRPAGLEAEVLDLCLTIPGVTRNTLAKLMDLPTLAARLAFLEKFKPKDPAVEVP
                     KRQPPTKVKPNQFRFSQTQPGASFQVEVKYRKRDASLPEIIAALELALAEANRQATEK
                     VSV"
BASE COUNT          935 a         1019 c          981 g          944 t
ORIGIN      
        1 gtggtgaccg gtaaaagctg gagcgaattt gtcacagaac ggatcctgac tccacttgga
       61 atgacctcaa ccacgacaag cgtcactgcc ttcaaacctg gtgacaacgt tgctgcgccg
      121 cataatgaac tcgacggaat gatgcgcgtg attcgctatt caaatgttga tagtggtgcg
      181 gctgccgccg ggctcaattc ctgtgtggct gatatagcgc agtggctcag gctgcaactg
      241 gggcggggaa gatatcaggg caaagtcatc ttcagtgcca aaaattcccg cgaaatgtgg
      301 acggcgcaca ccctccaacc catcagcgaa ctggctgaaa agctcaatcc gacacagcat
      361 tttagcgcct atggacttgg gtggtcgctc aacgattatc agggccgcaa agttatttcg
      421 cacggtggtg gactggatgg aatgatctcg cgcacggcca tgattccaga ggaaaacctc
      481 ggtctggttg tcctgaccaa cagtgaaacc gcactggcga acgtggtttc aaacaaaatt
      541 ttcgatgtgt ttttgggtgc tccccagcgt gactggagcg ctgaatttct ggaacttacc
      601 cgccgacgca aagaagccga aaaactggaa gcccagcaaa ttgaagcgtc ccgcattccc
      661 aatactcagc cgtcgcttcc cctggcgaaa tacgccggga aatattcggg gccgctctac
      721 ggcgatgcca ccatcacgga agaaaacggc aaactcgtac ttcggctcat tccagcgcca
      781 aatttcgtcg gagatttgga acactggcac ctcgacacct tccgcctgaa atggcggccc
      841 agtgtcagtt atccgttccc aaagggattt gtgaccttta ccatcaatgc cagtggaaaa
      901 ccaatcgaac tcaagattga ctgtccgaat ccggattttg attttggaga gttggaattg
      961 aagcgataga accttgtcag tcctgcctgt gtgagcggat gggctggaag cagttccacc
     1021 cgctcacgtt ggcgatactg ataccaattt gcaatggaat cctcagtatt aggaaatagt
     1081 caaatagttc aatcaaataa gcttagcgaa ctactgacta ctgactactg actacaaact
     1141 ggtatgacaa ttcctatttc gtttccggct tgacgtattt ttcagggttt ttgtcaaatt
     1201 tgggcttaca gccagggcaa cagaagtaat agcgtttgcc tttgtaatcg ctaaacgtag
     1261 ccgatgcggt gtccaggact tcttccttca tcaccgggca catcacgact tcttcttttt
     1321 ctggtttggg ttcgggtttg gtctggtcct gagcaaaagc cggggctgaa aaagacaggg
     1381 ccagggttgc cacagcggcg agcgtgagct ttcgaaacat ggttgattct cctgaaaaat
     1441 gggaatgaaa agatgaaaag cgttgattat tctcatctta gcccatctgc gccagcctaa
     1501 tttccaggaa cggagcgggc ttttttgggc ggagtagttt tggcaagggc aatccgctgc
     1561 tctgccagtt gatccgctgc tttgtaggtc gcaatatttt cacgctctgc atactcaaat
     1621 acttcacgca ctgtctggta aatagcttcg actttggaac gggcaaaatc aggtgtccaa
     1681 ccaaccagat ccacgccgcc actgagaatt cccccggcat ttacgacata atcgggagca
     1741 tacaaaatcc cccggtcctc cagttcatcg ccgctttgat cgtccaagag ctggttgttg
     1801 gcggcgccag cgacaatttc aactttcaac tgaggaatgg tctccgggtt aatgaccgca
     1861 cccagggcac agggtacaaa aatatcggct tcgactgaaa aaatatcggc tgggtcaact
     1921 gccgggacat ccagttcagc catcatgcgc ttgacccgat tctggctcac atcggtcacc
     1981 accagtttgg cgccagcctg atggagttgc cgcgccagat gcgttccgac attgccaagt
     2041 ccctgaaggg caattcgtcg tccggtcagg tcatcacttc cccaacgata attggctgcg
     2101 gcttgaattc cccgaaaaac cccacgcgct gtccagggcg aagggtctcc gttacggctt
     2161 gccaatccag cgacatggcg ggtttcctga tgaatcaact ccatatccgc tggactggta
     2221 ccgacgtcct ctgccgtgat gtacctcccg cccagcccgt caatcaagcg cccatgggcc
     2281 cggaacaaga cttcacggtc agtgcggcga ttgtccccga tcaaaactga cttgccacca
     2341 ccaagttgca accctgccag ggcgttttta taggtcattc cgcgtgccag ccggagcgca
     2401 tcaacaatcg cgtcctcttc gctttcataa ttccaaaatc tggtgccacc cacagcgggg
     2461 ccaagggcag tgctatgaat ggcaataatg gctttgtatc cgatatttga atcctgagca
     2521 aacacaagct gctcgtgacc aaattcttgc aaatgttgaa atagcttcat atacacctcc
     2581 tggctgaaag aatttataac tatcacatga aatgaaaaac gtgacgattc ttgcaggtta
     2641 ccattccttt tggtacccga aaagttggaa tggctatcct atacacgcct caaccagaaa
     2701 aataaagcaa tttctttgcg caaaaaaaag cagtcaaaac ctccgtcttt tgcggcccca
     2761 acatccagtc agatcagaac ttgcgggcga aacccacata gaagacagca cagcctgact
     2821 ttgcctggcg gattttatgc aaaccagtct ctggaaacta ttcaaaaaat tagatttagg
     2881 ccattgttca accttgaaca atttcaattt gcctggctta cacactcacc ttttcagttg
     2941 cctgacgatt ggcttcagcc agggcgagtt caagcgcagc aataatttct gggagcgatg
     3001 cgtcccgttt gcgatatttg acttcaacct ggaacgaggc gccaggctgt gtttgtgaga
     3061 atcggaactg gtttggtttt acttttgttg gtggctgacg tttcggaacc tcaactgccg
     3121 ggtctttggg cttgaacttc tccagaaaag ccagtcgcgc agccagtgtc ggcaaatcca
     3181 tcagtttggc gagtgtgttg cgggtcaccc ctgggattgt taaacacagg tcaaggactt
     3241 cggcttccaa cccggcggga cgaatcaaac gggccagtgt tgagcgatga aagccgacca
     3301 gtcgagcggc ttcttcctga gtcgcgcact gttcgacaat ccgggccagt gcctggactt
     3361 cttcaactgg atgcaaatcg gctcgctgaa ggttctcaac cagcgccagc agtccagtat
     3421 ccgtggtttg gtttccacca cgcagcacca tcggtacccg gctcaggttt aacgcctggg
     3481 ctgcccgcca tcggcgttcg ccggcaatca gtgtataggt tccgtctgag gcttcctggc
     3541 caatgagtgg ctctaaaatg ccatgttcgc ggatactggc ggcgagttca tcaatggtct
     3601 cagggtcaaa tatttttcgc ggctgatgcg gattgggccg aatctgcgac gtctcaacgt
     3661 gcaacagcac atcaccgctc tggctggcga cagattgata ggcaaggttg gtcatcaatt
     3721 gcttcggtaa tcgaaccgga ggtttctttt tgctcataca ggtttggggc tgaagaagac
     3781 gggctgaaga agacgggctg aagaaggcgg gctgaagaag gcgggctgaa gaattggttt
     3841 tctttcatcc ttcatccttt atccctcgtt ccctggttt
//
LOCUS       DAOQDO010000288         3877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16695_length_3877_cov_2.766614, whole genome shotgun sequence.
ACCESSION   DAOQDO010000288 DAOQDO010000000
VERSION     DAOQDO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16695_length_3877_cov_2.766614"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PKZ53_05920"
     CDS             <1..178
                     /locus_tag="PKZ53_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNJ40007.1"
                     /translation="GISSGSAKPNRDKVGKITSKQLREIAESKIQDMNCGSVEAAIRT
                     IAGTARNMGLEIVD"
     gene            328..1023
                     /gene="rplA"
                     /locus_tag="PKZ53_05925"
     CDS             328..1023
                     /gene="rplA"
                     /locus_tag="PKZ53_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNJ40008.1"
                     /translation="MAKVGKKYRAATAQIDATKLYQLPDAMQLVSKIAFAKFDETVEV
                     TMVLGVDPRKADQMVRGTVVLPNGLGKSKRVCVIATGEKQREAREAGAEEVGGEELVD
                     RIKGGWLDFDAVIATPDVMKQVGTLGKILGPRGLMPNPKTGTVTFDVAKAVQETKAGK
                     VEYRVDKTGVIHAPIGKVSFGSQKLLENAKVLIDAVIKAKPQTAKGRYVKGMHLCSTM
                     GPNVRLDAASFHV"
     gene            1045..1587
                     /gene="rplJ"
                     /locus_tag="PKZ53_05930"
     CDS             1045..1587
                     /gene="rplJ"
                     /locus_tag="PKZ53_05930"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNJ40009.1"
                     /translation="MNRTEKNQELEVLTKSFQSTPHAFLVGFQGLTVGVDTNLRAEMR
                     KANITYKVVKNTLARRAAKGTALEVVEKNFVGPTAVAIHPEDPVTTAKLLQKFFKENP
                     KFVFKAAVVEGRAIQVTELDELANMPSREELLSKLLFVLNSGAQRLASVTNGVARNLA
                     IVLNQIHEQRANAENPPAAE"
     gene            1792..2172
                     /gene="rplL"
                     /locus_tag="PKZ53_05935"
     CDS             1792..2172
                     /gene="rplL"
                     /locus_tag="PKZ53_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNJ40010.1"
                     /translation="MSEKLQAILASVESLTLLETAQLVKMMEDRFGVSAAAATAVAAA
                     PAAAAAAAPVEEKTEFDVILTGAGANKINVIKVVRELTSLGLKEAKDLVEGAPKAVKE
                     GVNKEEAEAVKKKLVDAGATVEVK"
     gene            3110..>3877
                     /locus_tag="PKZ53_05940"
     CDS             3110..>3877
                     /locus_tag="PKZ53_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNJ40011.1"
                     /translation="MSLTTNPTPTERHDFSKIKTTIRIPNLIEVQRESYNRFLQMDLL
                     PDERESIGLQSVFRSVFPINDFRETAQLDFVDYSIGEWKCKCGKNEGLKFLRSNCHSC
                     GSTIRIDPLSGEDIVCGTCGSLNKNIVTLCDVCGEPVGLKHKYDVQECQERGMTYAVP
                     LKVKIRLTVWDKNEETGKRSLRDFRDEDVYFGDIPLMTEKGTFIINGTERVIVSQLHR
                     SPGVFFEKSPVYLAKVIPYRGSWIEFEYDQKNLLYVRI"
BASE COUNT          990 a          918 c         1014 g          955 t
ORIGIN      
        1 agggatttct tccggttcgg ccaaacccaa tcgcgacaaa gttggcaaaa tcacaagcaa
       61 acagttgcgc gaaattgcag agtcaaaaat ccaggatatg aattgtggtt cagtcgaagc
      121 tgcaattcga accattgctg gaaccgcccg gaacatgggc cttgagattg ttgactaatt
      181 tcggttttct tgatgatcac cctcgatttt gccaccgagg gtgatttttc attctaaagc
      241 cacctctaga gttgggtgct tcgatgagca tccagggagt agttccggcg gcaacgccgg
      301 ctacgtttgt acctgaaagg acattgtatg gcaaaggttg gaaaaaaata tagagccgca
      361 acagcgcaga ttgatgctac gaaattgtat caactgcctg atgcaatgca gttagtctca
      421 aagattgcct tcgccaagtt tgatgaaacc gtggaagtaa ccatggtgct tggtgttgat
      481 ccgcgaaaag cagatcaaat ggtgcgcggc acggttgtgc tgcccaatgg gcttggaaaa
      541 tcaaaacggg tgtgtgtgat tgccacgggt gaaaagcaac gcgaagctcg cgaagctggt
      601 gctgaagaag tcggcggcga agaactcgtt gaccggatca aaggtggctg gcttgatttt
      661 gatgcggtga ttgccactcc ggacgtgatg aaacaggtcg ggacgctcgg taaaattctt
      721 ggtccgcgtg gtttgatgcc aaacccgaaa actggcaccg taacctttga tgtcgccaag
      781 gcagttcagg aaaccaaggc tgggaaggtc gagtaccgtg ttgataaaac aggtgtgatc
      841 catgccccaa tcggcaaagt ttcctttggt tcacaaaagt tgcttgaaaa tgccaaagtg
      901 ctcattgatg cggtcatcaa agccaaacca cagactgcca aagggcgcta tgtgaaagga
      961 atgcacctct gctcgacgat ggggccgaac gtgcgtttgg atgcggccag tttccacgtc
     1021 taaagggagt gaaaagaaaa acctatgaat cggactgaaa aaaaccaaga gcttgaggta
     1081 ttgaccaaaa gctttcagag caccccacat gccttcctgg ttggatttca ggggttgact
     1141 gtcggggtgg ataccaactt gcgcgctgaa atgcgcaaag ccaacattac ctacaaagtg
     1201 gtaaaaaaca ccctggcccg acgtgcagcg aaaggcacgg cgctcgaagt ggttgaaaag
     1261 aactttgttg gcccaacggc ggtggcgatt catccggaag atccggtgac cactgccaaa
     1321 ttgctgcaaa agtttttcaa agaaaacccg aagtttgttt tcaaagccgc tgtggttgaa
     1381 ggccgtgcaa ttcaggtgac tgaacttgac gaactggcca acatgccatc ccgtgaagag
     1441 ttgctgtcca agctgttgtt tgtgttgaat tcaggcgccc agcgcctggc ctccgttacc
     1501 aacggtgtgg cacgcaacct ggcaattgtg ctcaaccaga ttcacgaaca gcgagccaat
     1561 gcagaaaatc ctcctgcggc tgagtagcgt tgcaccgggg aatgacccaa agtttttttg
     1621 tttttatcgt ttcaaacaac gccgcggtct gtcagaggcg tgcgttggga gcctgaatag
     1681 agactttcga ggagatgcac ctttggagac tcgttcactg gcggtactga taccgaccgc
     1741 tgggaatcct ggaatcttac ctgttttgat cgttttttgg aggaagagat aatgtctgag
     1801 aaattacaag ccattttggc aagcgttgaa tcattgaccc tgttggaaac tgcccagttg
     1861 gtcaagatga tggaagatcg ttttggcgtg tcagccgcgg ctgccacggc ggttgcggca
     1921 gcccctgcgg cagccgcagc agcggctccg gtcgaagaaa agaccgaatt tgacgtgatc
     1981 ctgaccggcg ctggcgccaa caagatcaat gtgatcaaag tggtccgcga attgacctca
     2041 ctcggcctga aagaagccaa agatctggtg gaaggcgcgc cgaaggctgt taaagaaggc
     2101 gtcaacaaag aagaagccga agctgtcaag aagaaactgg tggatgccgg cgcaactgtc
     2161 gaagtcaagt agttgtcttg caagtcgtct gactgcctgt ggttcgtggt tttagccagt
     2221 aatgcggtaa agtgacttgc gcttcgccgg acaatgccta agcttagaag ggggaagtga
     2281 actcttcccc cttcagttca ttttcatcct ggatcgcaga atgtgtctct ggccgctttt
     2341 attcagagag gagcgccacc atcctcactg gaatcgttcg aacaacacgc cataccatct
     2401 ctgtttttca aacctctgat aatcatattt tctcagagga ggacccgctg acctcacaaa
     2461 atagtcagct tcatgatttc caaaataata tggcagtgct ccaaatccga cctggagtac
     2521 ccgtttctga tcagttgcgg cgattttggc cgcgacccat tagaaactat tcatcaggtt
     2581 ggggccttgg ttgcttttag ttctacaacg cgcttgaaga gcgtgctgag aaaagtgtct
     2641 cagtacggtc ttccattttg tgtttaagcc acttttttga ttgatattcg gttgattgac
     2701 tttccccggc aatgtgagcg gcgagttggt aagcccaatc aaccagcccg aaggtcgttt
     2761 cctgagagta ccaccatttt ggaatgatca agtcgtgtga gccaaaggtt aaagagttgg
     2821 aaaccaggtg tttcctttag cccctggccc aataccctca accctaaatg tgtagaacac
     2881 cttcatcctg gcttttctgg ataatccgct ccaaatgcca gtcactggtc cctgtaccac
     2941 tccagtttga tcagccatgt gggacaaaat cgccattatg ttgcttcttt tcctgccaca
     3001 gcagactgtc cggctggctg gatcactcct tcgccgttca tccgattttc caacttacag
     3061 tttggcgcac cagcgcacat gcaaacagag gtgaaacgct gctatgtcga tgtctctgac
     3121 aactaaccct acgcctactg agcgccacga tttttccaag atcaaaacca cgattcgcat
     3181 tccgaacctg atcgaggttc agcgcgaatc ctacaaccgt ttcttgcaaa tggatttatt
     3241 gcctgatgaa cgcgaaagta tcgggctcca gtctgttttt cgatcagtgt ttccgattaa
     3301 cgatttccgt gaaaccgcac aactcgactt cgtggattac tcgattggag aatggaagtg
     3361 caaatgcggg aaaaacgaag gtctgaaatt tctgcgctcc aactgccaca gttgtggctc
     3421 gacgattcgc attgaccccc tttcgggcga agatatcgtg tgtggaacgt gtggttcctt
     3481 aaataaaaac attgtcacct tgtgcgacgt gtgcggcgaa cctgtcggct tgaagcataa
     3541 atatgacgtg caagagtgcc aggaacgtgg gatgacctat gcggttccac tgaaggtcaa
     3601 aatccgattg acggtgtggg acaaaaacga agaaaccggg aaacgttcgc tccgtgactt
     3661 ccgtgatgaa gatgtgtact ttggcgatat cccgctgatg actgaaaagg gtacatttat
     3721 cattaatgga accgaacgcg tgattgtgtc gcagttgcac cgcagtcctg gggtgttctt
     3781 tgaaaagtca ccggtttacc tggccaaggt cattccatac cgcgggtcct ggattgaatt
     3841 tgaatatgac cagaagaacc tgctctatgt ccgaatt
//
LOCUS       DAOQDO010000289         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16728_length_3872_cov_3.390621, whole genome shotgun sequence.
ACCESSION   DAOQDO010000289 DAOQDO010000000
VERSION     DAOQDO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16728_length_3872_cov_3.390621"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PKZ53_05945"
     CDS             complement(<1..873)
                     /locus_tag="PKZ53_05945"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNJ40012.1"
                     /translation="MALGVTEDITELIGKTPLLRFKHIVPAGAANVYGKLEYLNPGGS
                     IKDRAAMGLITDAERQGFLKPGGTIIEPTAGNTGVGLALIGRARGYRIVLCVPEGYSI
                     EKMKVTQALGGEIVYTPNELGMQGAIDKAFELSKTIPDSFVPQQFSNPANPRYHYETT
                     GREIYEQLDGKVDAVVIGCGSAGTFSGVARYVRERNPLAVCIAVETQGSVLGGGQLGP
                     HRVEGIGTSFIPGNFDQEVCNKIVAVMDDDAFATVRALAAQEGVLCGGSSGANVFAAI
                     QLAKQLGPGKNIVTI"
     gene            complement(1329..1991)
                     /locus_tag="PKZ53_05950"
     CDS             complement(1329..1991)
                     /locus_tag="PKZ53_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40013.1"
                     /translation="MNERWKRLLLGFGIMFGMQFLAAYLGLKLGQSFPETTFYIMLGF
                     TLAAYFGGGLVMGLLADHVIILEPLLVSLLAITLNTTISALSTSQAGDGTLQFADVLL
                     MAYALKKGAVMSLLLAMTAVVIAALAGALVGERIATPTYDWISTAAPIVGLVSLVIGP
                     IFLLAFSGTEKGNPAVPWPFLIIIMLAFVIVVGVGYLLFTRERNEAEEISISPDHRRD
                     LS"
     gene            complement(2358..3092)
                     /locus_tag="PKZ53_05955"
     CDS             complement(2358..3092)
                     /locus_tag="PKZ53_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNJ40014.1"
                     /translation="MAHENGRPSRPTSSHRGKVAIFIDGNNLFHAARSVGVEIDYAKL
                     LDILRGDDTLLRAFFYTGVDQQAERQQGFLLWMRRNGYRVVEKELKTFADGTKKANLD
                     VEIAVDMLSLADKYDTAILVSGDEDFAYAVNAVAYKGVRVEIAGFRGNTSPRLIDVAD
                     RFIELDLLVHEIAKTDRYNTGNLHRRYPPEWAGERSTSGALRQDWNGSERTPSGSLNQ
                     PRQEWPSDRQMSEAPPTAPLEPHDVS"
     gene            3287..3583
                     /locus_tag="PKZ53_05960"
     CDS             3287..3583
                     /locus_tag="PKZ53_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNJ40015.1"
                     /translation="MLPYLEKSEGITFQVKVQPNAPRTRILGQHDTALKLALAAPPVD
                     GKANAECIRFLAKFFDLSRSQVDILTGETTRNKVILLRGFSAQAFEAKLNELEG"
     assembly_gap    3601..3700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1004 a          951 c          903 g          914 t
ORIGIN      
        1 aatggtgaca atgtttttcc caggccctag ctgctttgca agctggatgg cagcaaatac
       61 attggcgcca ctcgacccac cacacaacac accttcctgg gctgccagag cacgcacggt
      121 ggcaaaggca tcatcatcca ttacagccac aatcttgttg cacacttcct gatcaaaatt
      181 acctggaata aaactggtgc caatgccttc aactcgatgt ggtcccagtt gcccaccgcc
      241 caaaaccgag ccctgggtct caaccgcaat acagacagcc aggggattgc gctcacgcac
      301 ataccgcgcc acgccactaa aggtgccagc cgaaccacac ccgatcacca ccgcatccac
      361 tttcccgtca agctgctcat aaatctcacg gccagtcgtt tcatagtggt agcgcggatt
      421 tgccgggttg ctaaattgtt gcggaacaaa tgagtcggga atcgtctttg acaattcaaa
      481 cgctttatca atggccccct gcatccccaa ttcatttgga gtgtagacaa tttcaccacc
      541 caaagcctgg gtcactttca ttttctcaat gctgtatcct tcggggacac ataagacaat
      601 tcggtagccc cgggcccgcc caatcaaggc caggccaacg ccggtattgc cagccgttgg
      661 ttcgataatc gtgccaccag gttttaagaa cccctgtcgt tcagcatcgg taatcaaccc
      721 catcgccgca cgatctttga tgctaccacc agggtttaaa tattcaagtt ttccgtacac
      781 attggctgct ccggctggaa caatgtgctt gaagcggaga agcggggttt tcccgatcag
      841 ttcagtaata tcttctgtta cacctagagc catagcttta caagcgcctt tcgtttaccc
      901 gttcgaagat ttttggctga atcaaccaaa attgctgcgc agtcctccag cccatcatcc
      961 gcagtcgaat tcctctgtgg cggatttgat gaggaatgaa gaacggcatc catttcagtc
     1021 tgtcgtgccg acaattgcaa acacctctgc ccatcgcggt taggattcag gggagaggtt
     1081 caaaacaagc accaaagttg taaccggctt cttctaaccc ggacctcaga agaaaggatg
     1141 agggatatgt agacgggtgg ggtgaaaaaa aatgtgcaag ataaaaagat cgccagctaa
     1201 acagttggga agactgtagc tggcgatatt acctcagggg ggaaagggtg tcaaattacc
     1261 cccaattcgg tggcttgaaa aaacagaaaa gcctgagtgg tttccctatt ttattgacca
     1321 agcaagagtt acgacaggtc acggcgatga tcagggctga ttgaaatttc ttcagcctca
     1381 ttacgctcgc gggtgaagag caaatacccg acgccaacca caatcacaaa ggccagcata
     1441 atgatgatca aaaatggcca ggggactgcc ggatttcctt tttcagtgcc cgaaaaagca
     1501 agcagaaaaa tcgggccaat cacgagactg accagcccaa caatcggagc ggcagtgctg
     1561 atccagtcat aggtcggtgt ggcaatgcgc tcaccaacca gagccccagc cagcgcagca
     1621 atcaccacgg cggtcatcgc caaaagaagg ctcatcacgg cgcctttttt gagtgcgtag
     1681 gccatcaaca gcacgtcggc aaattgaagg gtaccatcac cggcctggct ggtacttaat
     1741 gcggaaatgg tcgtgttgag ggtaattgcc agtagcgaaa ccagcaacgg ctctaaaata
     1801 atcacgtggt cagctaacag ccccatgacc aaaccaccgc caaaataggc tgccagcgta
     1861 aatcccaaca tgatgtaaaa agtggtttct gggaaacttt gtcccagttt gagtccaagg
     1921 tatgcagcca gaaactgcat gccgaacata atcccaaacc cgagcagcaa tcttttccat
     1981 cgttcgttca tggtgggtct cctctacggt gaaactgtca cgatgttggt cttgctccaa
     2041 aatgtgttgc aggtagccgc ctacctgagt cagatcattg taaggtactg cgagtgcaaa
     2101 gcctgaaggg gtgacaagga atgaaatgag attctgtttg gtgttacgaa atacagggta
     2161 ccgatgtttg tccaagtttc ccaaaatctt ggttttaaac gggtttattg cggatagcaa
     2221 tgtacgttga tgcactgact ggtgtcaatt gcctggtgag tactcaaaac gagaaatcaa
     2281 aggtgcgaat tctagactga acgatggttt aaatttttgt ttttcaaaga agcaatccct
     2341 gcattccatc agacgggtta cgaaacatcg tgaggctcca gcggcgctgt cggaggagct
     2401 tcagacatct gtcggtctga aggccactcc tggcgaggct gatttaacga gccgctgggt
     2461 gttcgctcag atccattcca gtcttgccgt aaggccccgg atgtggaacg ctcgccagcc
     2521 cattcaggtg gatagcgacg atgcaggttg ccggtattgt agcggtcagt tttagcaatt
     2581 tcatggacca gtaaatcaag ctcaataaac cgatcagcga catcaatcag gcgtggtgaa
     2641 gtattacctc gaaacccggc aatctcaacc cgtacacctt tgtaggcaac ggcattgact
     2701 gcgtaggcaa aatcttcatc tccgctgacg agaatcgcag tgtcatattt atcagccagt
     2761 gacagcatat ccaccgcgat ttcgacatcg aggttggctt tcttggtgcc atcagcaaac
     2821 gtttttaatt ctttttcaac gacccgatac ccattgcgac gcatccaaag caaaaatcct
     2881 tgttgccgct ctgcctgctg atctacgccg gtataaaaaa aggcccggag tagcgtatca
     2941 tccccccgaa gtatatccag cagtttggca taatcgattt caactccaac ggatcgtgca
     3001 gcgtgaaaga ggttgttccc atctataaaa attgcaacct tacctcgatg agaagaggta
     3061 gggcgacttg gacggccatt ttcgtgcgcc ataggaaatc tcctaacgga aggcgacatg
     3121 cagtgaacaa aatacttggt gaaggatgag ccaaaatgat tgtatccttc gctccgtgta
     3181 aaggtcaatt caggagtctg aaaaccgtcc agacttttca caagtttgct caaatacccg
     3241 aatctggccc aataaattac tttttttcaa tatcttgaaa ttttttatgc ttccatatct
     3301 ggaaaaatct gaggggatta cttttcaagt caaagtccag cctaacgccc cacggactcg
     3361 aattttgggt cagcacgata cagcactgaa actggcgctg gcagcaccgc cggttgacgg
     3421 gaaagccaat gctgaatgta tccggtttct ggcaaaattt tttgaccttt cccggagtca
     3481 agtagacatc ctgactggcg aaaccacacg caacaaagtt attctgttac gtggtttctc
     3541 agctcaggca tttgaagcca aactcaatga gcttgaaggc tgaagtgggg ctgaagacat
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgggcttggg gctgaagaca
     3721 ttgggcttgg ggctgaagac attgggcttg gggctgaaga cattgggctt ggggctcagg
     3781 gttgaagact tcgggctgaa gaaatttgat tgaggcggtt cttcattctt cattcttcat
     3841 tcttcattct tcattctcaa ttcttcattc tt
//
LOCUS       DAOQDO010000290         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16848_length_3858_cov_2.458322, whole genome shotgun sequence.
ACCESSION   DAOQDO010000290 DAOQDO010000000
VERSION     DAOQDO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_3858_cov_2.458322"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /locus_tag="PKZ53_05965"
     CDS             <1..2238
                     /locus_tag="PKZ53_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ40016.1"
                     /translation="AALQRTSPTIGGIYVRGLTGKNVVVFIDGVRYSNSAQRGGINTF
                     LNLIEPSNLRSVELLRGPNSSQYGSDSLGGSVQFVSSPATFSDSGFDFHGQTSLFANS
                     ASATFGQATRFGFGGTNYGFLVNLASRRTNTLRPGNGIDTHAAVTRFLGLPSDIINGN
                     RLPDTAFTEYGGNIRFNYAFGGGNQLIFNYQRSQQDGGKRYDQLLGGDGNNIAELKNL
                     MLDFGYVRFDTQRLPFADNFAVSFSFNNQREERINQGGNGNPLGSITHQPERTRTYGV
                     QFFGNKRLGNHNVLIGGDYYHDRLTSPAFVVSVVSNSSTLSRPRIPDNAFYHNYGFYA
                     QDVWQAIPERLNISASVRYGAASYKARAADSPLVKGERLWPDDSLRADQVTFRTSAVL
                     KLIGGLSLGGNISRGFRAPHMTDLGTLGLTGDGFEVAAPDVLGLGAQVGNSASSKAVT
                     TGRPVTQVGPEISKNYELSLRFRNDWLDTDLGGFITDIDDNIQKQALILPQGAVGKSL
                     GGEIITSQLPSGVVFVDASTNPVLVRSNFDQVRMWGLEYTLDAKLNTAWSIGGNFTYI
                     YARDKANGLPPNIEGGTPAPNGTLRLRYNPPGKRYWVEVSGFGAYRQDRLSSLDLSDR
                     RTGANRSRTSIRNFFYNGATVRGLVAAGADGRFGTADDILRQTGETLEQVQNRVLGKL
                     EQSPLFPYVPGFGLLNVRGGIRLADNQELFLALENITDASHRGISWGIDGPGINFTAR
                     YTIKF"
     gene            2413..3801
                     /locus_tag="PKZ53_05970"
     CDS             2413..3801
                     /locus_tag="PKZ53_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNJ40017.1"
                     /translation="MSVRIALFGGSFNPPGIHHREIARLLSLHFDRVIIVPCGPRPDK
                     RTTNDIEPIFRATLTDLNFRGLPKVEVDLFDLEQATFTRTHDLQDRYASQGDVWHVVG
                     GDLIKGGKDGASFIQTTWKNGAAIWNHLNFAVITRDGIDFDERDLPPHHQIFHIQVSG
                     ASSSIRERIFNHQPIEELVTPAVSEYIERYELYRGRIPSRLTRYQFENPRLLIVPDET
                     NPRAMSLVPRFEPYRDDQNPNCILVIGGDGMMLGAISQYWHLRLPFFGVNAGHLGFLL
                     NEANEVLNGSFRPQELLIRQMPLLYVEAQLEDGTLVSSLSFNDAWVERATSQVGWIEV
                     KVNGRVKIPKLIADGALVSTAAGSTAYARAMGAMPLLADTPALLLVGSNVLEPPGWKS
                     ALLSLDSEVEFCTLSPIKRPLEGFAGNNSLGKVQKLQIRVSRIAAAELAFCPTHDMAE
                     KIAQIQFPNQHG"
BASE COUNT          875 a          948 c         1061 g          974 t
ORIGIN      
        1 gccgcgcttc agcggaccag cccgacaatt ggtggtattt atgttcgtgg gttaactggc
       61 aaaaacgtcg tggtgtttat tgacggcgtc aggtattcaa attctgccca acgcggtgga
      121 atcaatacct ttctgaatct catcgaacct tcaaacttgc ggagcgtcga gttgttgcgc
      181 gggccaaaca gttctcaata tggcagcgac agtttgggcg gaagcgttca atttgtgtca
      241 agcccagcta ccttttccga ctctggattt gactttcatg ggcaaaccag cctgtttgcc
      301 aattctgcga gtgccacctt tggtcaggcg acccggtttg ggtttggcgg caccaattat
      361 ggatttttgg tgaacctggc gtctcgtcgc accaataccc tgagacccgg aaacggaatt
      421 gatacgcacg cggcggtgac ccgatttttg ggacttccgt cagatatcat caatggaaac
      481 cggctgccag atacagcctt taccgaatat ggtggaaaca tccggtttaa ctatgccttt
      541 ggcggcggaa atcagcttat tttcaactat cagcgcagcc agcaagatgg tggaaaacgc
      601 tatgaccagt tgctgggtgg tgacggtaac aatattgccg agttgaaaaa cctgatgctg
      661 gattttgggt atgtccgatt tgatacccag cgcctgccgt ttgccgataa tttcgcggtg
      721 agtttttcgt ttaacaatca gcgtgaagaa cgcatcaacc agggcggaaa cggaaatcca
      781 cttggttcaa tcactcatca gccggaacgg acccgaacct acggagtcca gttttttggc
      841 aacaagcggc tgggcaacca taacgtgttg attggcggtg attactacca cgaccggctg
      901 acctcgcccg cgtttgtggt gagtgtggtt tcaaactcca gtaccctctc gcgtcccaga
      961 attccggata atgccttcta ccacaactat gggttttatg ctcaagatgt atggcaggcg
     1021 attccagagc ggctcaacat ttcagccagt gttcgatatg gagcggcgtc gtacaaggcc
     1081 cgtgcggcag atagcccgct ggtcaaaggg gaacgtctct ggcccgatga ctcattacgt
     1141 gctgatcagg tgacgttccg aaccagcgct gtgctgaaat tgatcggcgg cctgtcactg
     1201 ggtgggaaca tcagtcgtgg atttcgggcc ccgcatatga cggacctcgg gacgctcggg
     1261 ttaaccggtg atggatttga agtggcggca cctgatgtgc tcggactggg agcccaggtg
     1321 ggaaattccg ccagctcaaa ggcggtcact actggtcggc cagtgaccca ggttgggcca
     1381 gaaatcagta aaaattatga attgagcctt cggtttcgca atgactggct cgatacggat
     1441 ctgggcgggt ttattacgga tattgacgac aacattcaaa aacaagccct gattttgccg
     1501 caaggcgccg ttgggaaatc gcttggtgga gaaatcatca ccagtcagtt accttccggt
     1561 gtggtttttg ttgatgcctc aaccaacccg gtactggtca gatccaactt tgatcaggtt
     1621 cggatgtggg gacttgaata cacgctggat gccaaattga atacggcctg gagcattggt
     1681 ggaaatttca cctacattta tgcgcgtgac aaagccaatg gattgcctcc aaatattgaa
     1741 ggcggcaccc cggcaccaaa cgggacgctt cggctacgct ataaccctcc aggaaagcgc
     1801 tattgggttg aagtttctgg atttggagcg tatcggcaag atcgcctgtc atcgcttgat
     1861 ttgagcgacc gccggactgg cgccaatcgc tcacggacct caatccgcaa tttcttttat
     1921 aacggagcga cggtccgggg tctggttgcc gccggtgccg atgggcgatt tggaacggct
     1981 gatgacatcc tgcgccaaac cggtgaaacg cttgagcagg tacaaaatcg ggtgttgggg
     2041 aaattagagc aatctccgtt gttcccctac gtccccggat ttgggttgct caatgttcga
     2101 ggaggcattc gccttgctga caaccaggaa cttttcctgg cgcttgaaaa catcactgat
     2161 gcgtctcatc gcgggattag ctggggcatt gatgggccag gaattaactt taccgcccgg
     2221 tacacaatca agttttagtg tgagtcaggt atcagccctg ccaatgaagg gttgaaattg
     2281 agatcccaaa ccagcaacgt acagccagca ctttgactgg ctgtacgttg ttgttttggt
     2341 acgtttgttg gtttcaacga acgatttttt caacgcttca ttggcatgac tcaggactgg
     2401 cttttttctt ttatgagtgt acgcattgct ctctttggcg gcagttttaa cccaccagga
     2461 atccatcacc gcgaaattgc ccggctgttg tcgctacatt ttgatcgggt cattatcgtt
     2521 ccctgtggtc cgcgaccaga caagcggaca accaatgaca ttgaaccaat ttttcgagcg
     2581 accctgactg atttgaactt tcggggattg ccaaaagttg aggttgatct ttttgacctg
     2641 gaacaggcga cctttacccg aacccacgat ttacaggacc gctatgccag tcagggcgat
     2701 gtctggcacg ttgtgggcgg tgacctgatc aaaggtggaa aagatggcgc ttcctttatc
     2761 cagacaacct ggaaaaatgg agctgcgatt tggaatcatc taaattttgc ggtgattacc
     2821 cgtgatggaa ttgactttga tgaacgtgac ctgccaccgc atcaccagat atttcatatt
     2881 caggtttccg gagcaagttc ctcaattcgg gagcgaatct tcaaccatca gccaattgaa
     2941 gaactggtca ctccggcggt ttctgaatat atcgaacgat atgagttata tcggggccgg
     3001 attcccagcc ggttgacccg ctatcaattt gagaatccac ggcttctgat tgtgccagat
     3061 gaaacaaacc ccagagccat gtcgctggta ccccggtttg aaccgtaccg ggatgatcaa
     3121 aacccaaatt gtatcctggt gattggtgga gatgggatga tgcttggggc catcagccag
     3181 tactggcacc tgcgactacc gttttttggc gtgaatgccg gccatcttgg gtttctgctc
     3241 aacgaagcca atgaagtgct caatggttct tttcgacccc aggaactgtt aattcgacag
     3301 atgccgctat tgtatgtcga agcccagctc gaagatggaa cgctggtgag cagcctgagt
     3361 tttaacgatg cctgggttga gcgggccacc agccaggtgg gctggatcga agtcaaagtc
     3421 aacgggcggg tcaaaatccc gaaactgatt gccgatgggg cgctggtttc aacggctgct
     3481 ggctcaacgg cgtatgcccg ggcgatgggg gcgatgccgt tgctggctga tacgccagcc
     3541 ttgctactgg ttgggtcaaa tgtgttagag ccgcctgggt ggaaatccgc cttgctgtcg
     3601 cttgattcag aggttgaatt ttgcacgctc agcccgatca agcggccact ggaagggttt
     3661 gccggaaata actcgctcgg aaaagtccag aaactacaga ttcgtgtcag tcggattgct
     3721 gctgccgaac tggccttttg ccccacccac gatatggctg agaaaatcgc ccagattcag
     3781 tttccaaacc agcatggcta atttggagtg cggtggcttg acaccgcttt gtggtctgcc
     3841 ggcttgacgg ctgtggag
//
LOCUS       DAOQDO010000291         3855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16871_length_3855_cov_3.285263, whole genome shotgun sequence.
ACCESSION   DAOQDO010000291 DAOQDO010000000
VERSION     DAOQDO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16871_length_3855_cov_3.285263"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKZ53_05975"
     CDS             <1..462
                     /locus_tag="PKZ53_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40018.1"
                     /translation="QIDQNNVSLPKEIRTILQSEYPPDYPQMAFHCVENPSLSEHYIL
                     FQKVTGHSDKDLELDLKFYEVTPATFEIKDNEIISETVFLGGAPNWTFACNQKTKELY
                     LLSGFNDPIIGFNQLMKKHNLDVSNEEVCLDLFDTYLLFACGSSLQRICYS"
     gene            786..1577
                     /locus_tag="PKZ53_05980"
     CDS             786..1577
                     /locus_tag="PKZ53_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40019.1"
                     /translation="MSFQIFAQRPAQRCDICHQSDCFDPITSQCSRCQPVVEQRLTSK
                     VTVRTGQRPASIPLLFQSDWFFQPYFPKSSRNQNPTLAPGLPWWKVVLPGLTTFEKLA
                     FYLLILALFPAWLVQQRVQNAITARFVLGHVVTMQDTACDLITFDNYTDNHLYYPVIQ
                     IQGCRHVNETPAEEINRKVGIYNGYRLIWKARKKTDSEPSSFTAVMVPLVQTGMFRTG
                     DDCFYIDESGVMRHSGSATVIPNRWSPVYERDFSQFGSWFYPVRY"
     gene            1612..2148
                     /locus_tag="PKZ53_05985"
     CDS             1612..2148
                     /locus_tag="PKZ53_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ40020.1"
                     /translation="MSFVLRLADHSDVSALQQLIPLSVRELSREVYTTDQIESAIVHI
                     FGVDTQLIDDQTYFVVEASGVMVGCGGWSKRKTLFGGDQTKATTVDSLLDPAIDAARI
                     RAFFVHPDWKRQGIGRLIITACESAAIQAGFRRFELVATLPGEPLYVAMGYCVTNRFE
                     IPMPDGNSLPASRMIKEL"
     gene            2156..2569
                     /locus_tag="PKZ53_05990"
     CDS             2156..2569
                     /locus_tag="PKZ53_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40021.1"
                     /translation="MTQILIEGYTSDEVFHFPLPELEALIFTGEPIELKIGSAKILAQ
                     FQKTECTLTLELAQIDGGGEGVLLTLALLVEKYAQLTSLTEIHWIVHAVHCAKPNLKL
                     RRMLIRKGFQLKELDGVGLVYFRNQMLYQFVVSSP"
     gene            2655..3632
                     /locus_tag="PKZ53_05995"
     CDS             2655..3632
                     /locus_tag="PKZ53_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ40022.1"
                     /translation="MEKTEPPKKPKLKADAMPAAPWPPAPKYYSGGFEATPHPTAGVI
                     TWNINTTCNYRCSYCTQRFLDNRARWARDTQAFLEGFGRLPGQWEVKLSGGEPFLHPT
                     LLEIVAGLESLGHLVSVVTNFSATDEKLLAFLHVAGKALRVFSASLHLEYVSDQAFLV
                     QFTEKARLVQDRLPECASLNITCVATRDNLSKLAELAAWFASQGLRFKIQPEKQNRDV
                     IEYTPEEQQHLVQLGGHNGLGKIAWSFQGRPCWAGAYSFTLDDLGNAWRCYPARRFRA
                     QYLGNFLTPAFVLAEGPSPCLYAYCNCTVPIERGMMPKTAGDIFHGNEV"
     gene            3622..>3855
                     /locus_tag="PKZ53_06000"
     CDS             3622..>3855
                     /locus_tag="PKZ53_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40023.1"
                     /translation="MKFKTFRNILIGGVLVSVAGIGACTVVSFYFISSRPAPSAPPPP
                     VAPATPPAPPIGLAPAAPTSPAPPAIPLAPGARR"
BASE COUNT          984 a          945 c          892 g         1034 t
ORIGIN      
        1 cagattgatc aaaataatgt ttcgctaccc aaagaaatta ggactatcct ccagtcagaa
       61 tatccaccag actatccaca gatggcattt cattgtgttg agaatccctc gttgagtgag
      121 cattatatcc tgttccaaaa agtaaccggt cattctgata aagacttaga gttggattta
      181 aaattctatg aagttactcc agcaacattt gaaattaagg ataatgaaat aatcagtgag
      241 actgttttcc ttggtggagc tccaaactgg acgttcgcct gtaatcaaaa aacgaaagaa
      301 ctctacttac tgtccggttt taatgatcca attatagggt ttaatcagtt aatgaaaaaa
      361 cataatttag atgtatcaaa tgaagaggta tgtcttgatt tatttgatac atatttgctt
      421 tttgcttgtg ggtcgagcct tcagagaatc tgttattctt gatgatttgc acctccaaag
      481 ccttgcactt caggattttt gcttgaccta ttccagaagt cagaagagaa agaaattcaa
      541 ttcctggtgg agaaaaatcc caggcgccct tatacaaaaa ttacaaccac tcaagccaac
      601 ctttaaagat gggaattatg aaattcgatt ttttagatac caacaaaaga aagttcttga
      661 agaatcaatc acgatgaatc gctcaggaat gatttcaact cataaatcaa ctctgattta
      721 ccctcaaaaa gacaagctcc cccaggatta acccgcagct tgtagactga atggaccttc
      781 atgctatgtc atttcaaatt tttgctcaac ggccagccca gagatgcgac atttgccatc
      841 agtcagattg ttttgacccg attaccagtc aatgtagccg ctgtcagccg gtagttgaac
      901 aaaggctaac gtcaaaagtt accgtgcgca ctggccaaag accggcctcc attcctttac
      961 tgtttcaatc cgactggttt tttcagcctt attttcccaa gtcatccagg aatcaaaatc
     1021 caactctggc acctgggctg ccgtggtgga aagttgtgct tccgggactc accacgtttg
     1081 aaaagctcgc gttttacctg ctgatcctgg cactgtttcc cgcctggctg gtgcaacagc
     1141 gcgtgcaaaa tgcaatcact gcccgctttg tacttgggca cgtagtgaca atgcaagaca
     1201 cagcctgcga cctgattact tttgataact atactgacaa ccatctgtac tacccggtca
     1261 ttcagatcca gggatgcagg catgtcaacg agactccggc agaagaaatc aaccgcaaag
     1321 tggggattta caatggctat cggttgattt ggaaagcccg caaaaaaacc gattccgaac
     1381 cttccagttt tactgctgtc atggtgccgc tggttcaaac cgggatgttc cgaaccggag
     1441 atgactgctt ttatattgat gaatctggag ttatgagaca ttcaggaagt gcaacggtta
     1501 ttccaaaccg gtggagcccg gtgtatgagc gagacttcag ccagtttggc tcgtggtttt
     1561 atcctgtccg atactaactt tttgatgaaa ttcaggcttc ccagtttgtg tatgtccttc
     1621 gtacttcgac ttgccgatca ttcagatgtg tctgccctcc agcaattgat cccgctttcg
     1681 gttcgtgaac tcagccggga ggtctacacc actgatcaaa ttgaaagcgc catcgtccac
     1741 atttttggcg ttgataccca actcattgat gatcaaacct attttgtggt ggaagccagc
     1801 ggcgtgatgg tcgggtgtgg tggttggagc aaacgcaaga cactctttgg tggagaccag
     1861 accaaagcca ctacggttga ttccctgctt gatccagcca ttgatgccgc ccggattcgg
     1921 gcattttttg ttcatccaga ttggaaacgc cagggtattg gtcgcctgat tatcactgcc
     1981 tgtgaatcag ccgccattca ggccggattt cgacgctttg aactggtggc aacgcttcct
     2041 ggtgagccac tctatgtggc gatggggtat tgtgttacca atcggtttga gattccaatg
     2101 cccgatggca attccctgcc agcttcacgc atgatcaaag aattataggc actctatgac
     2161 tcaaatcttg attgaaggct acaccagcga cgaggttttt cattttccgc ttcctgagct
     2221 tgaagcgctg atttttactg gcgagccaat cgaattgaag attggatcgg ccaaaatcct
     2281 ggctcaattt cagaaaactg agtgcacatt gacgcttgaa ctcgcccaga ttgatggtgg
     2341 aggcgaaggc gtcctgttga cactcgccct cctggttgaa aaatatgcac aactgacaag
     2401 cctgacggaa atacactgga tcgtccatgc cgttcactgt gccaaaccaa atctaaaact
     2461 ccggcggatg ctcattcgga aagggtttca actcaaagaa ttggacgggg ttgggttggt
     2521 ctattttcga aatcaaatgt tataccagtt tgtagtcagt agcccgtagt cagtagttca
     2581 ctaagtctat ttgaatgaat tacttgacca ttctctaatc cgaggctttt gttccaaaat
     2641 ggtattagat ttccatggaa aaaactgaac cgcccaaaaa accaaagctg aaagccgatg
     2701 cgatgccagc cgcaccctgg ccgccggcac caaagtacta ttctggcggc tttgaagcca
     2761 cacctcatcc aaccgcaggc gtcatcacct ggaacatcaa caccacgtgc aactatcgct
     2821 gtagctattg cacacaacgg tttctggata atcgcgctcg ctgggcgcga gacacccagg
     2881 cttttttgga aggctttggg cgattgcccg gacaatggga agtcaaactt tctggaggtg
     2941 aacccttttt gcatccgacg ctgttggaaa ttgtggctgg gctggaaagc ctgggccatc
     3001 tggtcagcgt ggtaaccaat ttttcagcga cggatgaaaa acttctggca tttctgcacg
     3061 ttgctggaaa agcgttacgg gtattttcgg ccagcctgca tcttgaatat gtctctgatc
     3121 aggcgttcct ggttcaattt acagaaaaag ccagactggt tcaggatcgg ttgcctgaat
     3181 gtgcatcctt gaacatcacc tgtgtcgcca cccgtgacaa cctgtcaaaa ctggctgaac
     3241 tggccgcatg gttcgccagc cagggtcttc ggtttaaaat tcaacctgaa aaacaaaacc
     3301 gggatgtgat tgagtacacc ccggaagagc aacagcatct ggtacagctt ggaggacata
     3361 acgggcttgg aaaaatcgcc tggtcgtttc aaggccgccc gtgctgggcc ggagcgtatt
     3421 ctttcacact ggatgatctc ggcaatgcct ggcgctgtta tcccgcccgt cggtttcgtg
     3481 cccagtatct tgggaatttt ctgacacctg catttgtcct tgctgaggga ccatctccct
     3541 gtttatatgc ttattgcaat tgcaccgttc caatcgagcg gggaatgatg ccgaaaactg
     3601 caggagatat ttttcatggc aatgaagttt aagacctttc gaaacattct gatcggtgga
     3661 gttctggttt ctgtggccgg aataggtgcc tgtacggtcg ttagctttta cttcatcagc
     3721 agccgaccag caccctcagc accgccacca cctgtggcac cagctacgcc accagcacct
     3781 ccgattggcc tggcgcctgc ggctcccacg tcgccagcac caccggcgat tccgcttgcc
     3841 ccaggtgccc gccgg
//
LOCUS       DAOQDO010000292         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16905_length_3850_cov_3.315415, whole genome shotgun sequence.
ACCESSION   DAOQDO010000292 DAOQDO010000000
VERSION     DAOQDO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16905_length_3850_cov_3.315415"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..526)
                     /locus_tag="PKZ53_06005"
     CDS             complement(131..526)
                     /locus_tag="PKZ53_06005"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNJ40024.1"
                     /translation="MNNRVAHKIWILFLLFCFGINFVTDVNAAADARRIMEMVYRQDT
                     SQDTAWQAFMDVYDKKGGLRRKKFVFRKLGALGNSKTLVRFLDPGEVRGVGLLTINQG
                     GQKDRQWLYTPAIQRTRRIAPQERDRRFS"
     gene            complement(519..3083)
                     /locus_tag="PKZ53_06010"
     CDS             complement(519..3083)
                     /locus_tag="PKZ53_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40025.1"
                     /translation="MLSALLSVTFKQWRTHRLRLAFTVLGIALGVSVFFAVKTANLTL
                     LGSLKTTVEKLAGKATLQVASGESGFPEEVLDVVRETPGVMIAEPVVETLAKTAFDPP
                     GNILIVGTDTTGDQSIREYQFDESQMELNDPLVYLSEPFSILVSRKFADRYQLKEGDK
                     LPLYTSRGKLSFNVRGIFAPAGIGAVFDGQIAVMDVYSAQYVFGRGKNFDRIDIQTEP
                     GVSVEQVQAALRSKLPAAIEVERPASRGKNIENVMAAMQQGMLITSYVALLVGIFIIF
                     NSFTISVNQRWKEIGILRSLGVERRNIALMFMGEALVIGVIGSLVGIVAGLYLAQGAA
                     KILSIVAATIFGLAAGVDQPVFRWDFAATTIVLGVLASLIAAWMPARAASQLNPVLAL
                     HNIEIRQPEVVAGWMRTLVGAILVTVAVLLVRFSPAQVGGMFQLVYSGILNFGLLLML
                     PKMAELIARSLRSPMDFLFGTEGVLAVDAMIHAPRRMNATVGALMFGLMFVYATGSYV
                     QSYKGVVERWMNRTINSDLFVKTTSEQVRSRSYHFSPEFSQKIATVPGVKRLENVRFI
                     TVEYRGTTPSLIALEMEGLFARTKDMVDEGDPVRAEAMACNGEGFLVARNFALRWKLK
                     VGDKVVLDTPTGVLERPIAGIIEDYTSDQGTVFMDRALYIQFWKDDGIDIVDINLQPG
                     VDVVAFKRELEKVISTEQQAFIYTNQEYKKWVFDLLDTFFFVIYLQTIISVLVAAIGI
                     FNTMMISVAERKRELGVLRAIGGLRGQVRKMVLLEAVVIAIVGLVTAIFAGAFNTYIL
                     VRLVALILGGFTIPFDLSFSLIFGTLPVVVVIALIAAYWPAHRAVNLKIVEAIGYE"
     gene            complement(3077..3766)
                     /locus_tag="PKZ53_06015"
     CDS             complement(3077..3766)
                     /locus_tag="PKZ53_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ40026.1"
                     /translation="MKVIELRNISKVYDSSKAVTALKNINFELNQGERVAIMGPSGSG
                     KSTLLNIIAGLDEPSAGQIVFEGRDISELNDDQRTRLRRERIGIIFQTFNLLSTLTAQ
                     ENVALPLRLKGIGRREAMDRAAKMLDRVRLTERQTHRPDELSGGERQRVAIARALIFQ
                     PPLLLGDEPTGNLDSKTGNEILALLDELHREFNTTILLVTHNAQAAEHCDQVLTMKDG
                     ELVSTGVAVVC"
BASE COUNT         1001 a         1066 c          915 g          868 t
ORIGIN      
        1 atcatcctcc aagtcggtgc gggaacgggt ttgctgtatt tgatgcgctg gttctggtgg
       61 cgcatcaacg cctggtgcga agtggcggcg atgattagct cctttctggt ttcggtcgcg
      121 tttctggcaa tcaagaaaag cggcggtcac gttcctgagg tgcaatccga cgggtgcgct
      181 gaattgccgg agtgtagagc cactgccgat ctttttgccc tccctggttg atcgtgagca
      241 ggccgacacc gcgaacttcg cctggatcca gaaagcggac cagcgttttg ctgtttccaa
      301 gcgcccccag tttccgaaag acaaatttct tgcgccgcaa cccacctttt ttgtcataga
      361 catccataaa tgcctgccag gcggtgtcct ggctggtatc ttgacgatac accatttcca
      421 taatccggcg ggcatcagcc gctgcattga catcggtaac aaaattgatt ccgaaacaaa
      481 acaataaaaa caggatccag attttatgag ccacacggtt attcatatcc aattgcctcg
      541 acaattttca ggttgacggc cctgtgtgcc ggccagtagg cagcaatgag tgcaatcacc
      601 accaccacgg gcagcgttcc aaaaatcaga ctgaaggaca gatcaaatgg aatggtaaag
      661 cctcccagga tcaatgccac cagtcgaacc agaatataag tattaaacgc cccggcaaaa
      721 atcgccgtca ccagacccac aatcgcaatc acgacggctt cgaggagcac catttttcga
      781 acctgcccgc gcaaaccgcc aatggctcgc aataccccga gttcgcgttt ccgttccgcc
      841 acggaaatca tcatggtgtt gaaaattcca atcgctgcca ccagcaccga aataattgtt
      901 tgcaggtaaa tcacaaaaaa gaaggtatcg agcaggtcaa aaacccattt tttgtattcc
      961 tggttggtat aaataaacgc ctgctgttcg gtggaaatga ctttttccag ttcgcgttta
     1021 aaagcaacca catccacacc gggctgtaaa ttgatgtcca cgatgtcaat tccgtcatct
     1081 ttccagaatt gaatatagag tgcccggtcc ataaacacag tgccctggtc agacgtgtaa
     1141 tcctcgataa tgccagcaat cggacgttcc agaacgccag ttggcgtatc aagcaccacc
     1201 ttatcgccaa ctttgagctt ccaccgaagg gcaaaatttc gggcgaccag aaatccttcg
     1261 ccgttgcacg ccatcgcttc ggcgcgaacc gggtcgcctt catccaccat atctttggtt
     1321 cgggcaaaca gaccttccat ttccagcgca atcaacgaag gcgtcgtccc acgatactca
     1381 accgtaataa agcgcacatt ttcaagtcgt ttgacacccg gcaccgtcgc gattttctga
     1441 ctgaattcag ggctaaagtg gtaactgcgc gaacgaacct gctctgaagt ggttttgaca
     1501 aacaagtcgg aattgatcgt gcggttcatc cagcgttcaa ctacgccttt ataactctgg
     1561 acataggacc cggtggcata gacaaacatc agtccaaaca tcagtgcccc aaccgtggcg
     1621 ttcatccggc gcggcgcgtg aatcatggca tcaaccgcca ggacgccttc ggttccaaac
     1681 aggaaatcca tcggtgatcg caaacttcgg gcaatcaatt ccgccatttt gggcagcatc
     1741 aacaacagcc cgaaatttaa aattcctgag tagacaagct gaaacatccc gccgacctgt
     1801 gccggggaga atcgaaccag cagcacggcc actgttacca gaatggcacc caccagtgtg
     1861 cgcatccatc cggcgaccac ttctggctgg cggatttcga tattgtgcag ggccagtacc
     1921 ggattgagtt gtgaagccgc ccgggcaggc atccacgcgg cgatgagtga cgccaaaacg
     1981 cctaaaacaa tggtcgtggc ggcaaaatcc cagcgaaaaa ccggttgatc aaccccggca
     2041 gccagtccaa aaatcgtggc ggcgacaata ctcaaaattt tcgccgctcc ctgagctaaa
     2101 tagagccccg caacaatgcc gacaagcgag ccaatcaccc caatcaccag ggcttcgccc
     2161 ataaacatga gggcaatgtt gcggcgctca accccgaggg atcgcaggat cccaatctct
     2221 ttccagcgtt gattcactga aatggtaaat gaattaaaga tgatgaaaat accgaccagg
     2281 agcgcgacat agctggtaat gagcattccc tgctgcatgg cagccatcac gttttcgatg
     2341 ttttttcctc gtgaagcagg tcgctcgact tcgatagccg ccggaagctt gctccgaagc
     2401 gctgcctgaa cctgttcgac cgaaacaccg ggttcagttt gaatatcaat ccggtcaaaa
     2461 tttttgccac gcccaaacac atactgggcc gaatagacgt ccatcaccgc aatttgccca
     2521 tcaaagacag cgccaattcc agccggagca aaaatgccgc gaacattaaa cgagagcttt
     2581 ccacgtgagg tgtaaagcgg gagcttatcg ccttccttca actggtaccg gtcggcaaac
     2641 ttacgcgaga ccagaattga aaacggctct gacaaataga cgagcggatc atttaactcc
     2701 atctgtgact cgtcaaactg gtattcacgg atagattggt cgccagtggt atcggttccc
     2761 acaatcaaaa tgttccctgg cggatcaaaa gccgttttgg ccagggtttc aaccacgggt
     2821 tcagcaatca tcacgccagg cgtttccctc acaacatcca ggacctcttc cggaaatccg
     2881 ctttcaccgg atgcaacctg gagcgtggcc ttgccggcca gtttttcaac cgtcgttttg
     2941 agcgaaccaa gcagggtcag attggcggtt ttgaccgcga aaaaaacgga aacacccagc
     3001 gcaatcccaa gaacggtaaa agcaagccgg agccggtgtg ttcgccattg cttaaatgtc
     3061 actgaaagta aagcacttag catacaacag ccactcctgt actgaccagt tcgccatctt
     3121 tcatcgtcaa cacctggtcg caatgttcag ccgcctgggc gttatgcgtc accagcaaaa
     3181 tggtggtgtt gaattcccga tgcagttcat caagcaacgc cagaatttca tttccagttt
     3241 tggagtccag gtttccagtc ggttcatcac ccagcagcaa cgggggctga aagatcaacg
     3301 ctcgggcaat agcaacgcgc tggcgctcgc cacctgaaag ttcatcgggg cgatgggtct
     3361 ggcgttcggt cagtcgcacc cgatcaagca tttttgcggc ccggtccatg gcttcgcggc
     3421 ggccaatccc ttttaaccgc aatggaaggg ccacgttttc ctgagccgtc aaagttgaaa
     3481 gcaaattaaa agtttggaaa ataatgccga ttcgttcccg tcgaagccgc gtccgttgat
     3541 cgtcgttgag ttcagaaata tcacgacctt caaatacaat ctggcctgcg gatggctcat
     3601 caagcccggc aataatgttg agcagagtgg attttcccga acccgacgga cccataatgg
     3661 ccactcgctc gccttgattg agctcgaaat tgatattttt caaagccgtt acggctttgc
     3721 tgctgtcgta cacttttgaa atattgcgta attctattac tttcattgca aaatcacctt
     3781 tccataggcg gagaaaagtc agtagtcagt agtcagtagt cagtagtcag tagtcagtag
     3841 tcagtagtca
//
LOCUS       DAOQDO010000293         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16916_length_3849_cov_3.589352, whole genome shotgun sequence.
ACCESSION   DAOQDO010000293 DAOQDO010000000
VERSION     DAOQDO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16916_length_3849_cov_3.589352"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKZ53_06020"
     CDS             complement(<1..324)
                     /locus_tag="PKZ53_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ40027.1"
                     /translation="MISIVLADDHHIVRQGLRALLEAESDFRVIGEASNGRDAIELVR
                     KVHPNVLVLDLMMPVLNGIEVTRQLGKAHPQTKIVILSMYANEAYVLETLANGASAYV
                     LKDSNT"
     gene            complement(546..2429)
                     /locus_tag="PKZ53_06025"
     CDS             complement(546..2429)
                     /locus_tag="PKZ53_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40028.1"
                     /translation="MKLPQLEWLNSENISELLKFGVEESLLAGLLESQSTLFQLANVF
                     LPFQNPATVFDFFPGFHRKSSEAEEELPDLSSRYQVLVEQIPAVVFMAFFDKGLSEAY
                     VSPHIESLLGFTQAEWLNDPVRWFEQLHPEDKTRWSMEAAHTFLTGDPLRSVYRVLAR
                     NGRVVWFHAEVKMVRHRDGRPWYIHGVAFEITELKEAEAALRSAHTDLEDRVRRRTRE
                     LADANFFLESEIAERKRAETELMKARDELEMRVRERTEELVFANVELRREIAERRRAE
                     TALSQSEEMLRAIFESAPDAMVVVNHRGQIERVNAQVEQMFGYRADDLAGQPVELLMP
                     ARFTQSHQEHRARYIRSPHLRDMGAGLELFARRRDGSEFPAEIMLSPIESETGRMVIA
                     VVRDISHRKQTEADLREYAERLQILSRRLMEVQEAERRRIALELHDEIGQALTGLKFT
                     LDLNARQASTEAQQKNIALSQQIVNELMARVRKLSLDLRPGMLDDLGLLPALLWLIES
                     YTTQTHVRIAFRHRGLKDKRLAPEVETAAYRIVQEALTNIARHAEVDEATIRTWVDHQ
                     TITIQIEDRGKGFDVDSVLSVPVSSGLAGMRERVLLLDGTFTIESKPGRGTRVTAELK
                     LQS"
     gene            complement(2426..3481)
                     /locus_tag="PKZ53_06030"
     CDS             complement(2426..3481)
                     /locus_tag="PKZ53_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HNJ40029.1"
                     /translation="MTEPTSSAVKIEQLDILWITAGLGCDGESVALTAATQPSIEDVI
                     RGAIPGTPEIRFFNQVYAYQTGDDFLKAFHEATTGNSRPFLLIVEGSIPNETNKESGY
                     WAGFGCDPETGQPILTCDWIDRLVPHAWAVMAVGTCATYGGIHALPGNPTGCMGLPDY
                     LGWDWKSKAGMAVVCVPGCPTQPDNLMQVLLYLLRQAAGYAPMIELDEALRPKWLFPD
                     SLHECCDRGGFYEQGQFAEQYGDAQCIVKQGCWGPVVQCNVGKRGWMSGVGGCPNVGG
                     ICIGCTMPGFPDKFMSFMNQPPGSLLSAQAVSTYGRAITALRKFTLGSMTQEPAWRKP
                     APGTPTLYSINSRKKNP"
     gene            complement(3478..>3849)
                     /locus_tag="PKZ53_06035"
     CDS             complement(3478..>3849)
                     /locus_tag="PKZ53_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNJ40030.1"
                     /translation="SEFTVPDQAIGCGFHEAVRGMISHHAVIRDGKIANYHPYPPTPW
                     NASPRDQFGTPGPFEDAIQNTPLFEENPPERFKGIDIMRAIRSFDPCMPCGVHMYLGN
                     GKVLETHHSLMLGHGGKGSKP"
BASE COUNT          934 a         1045 c          968 g          902 t
ORIGIN      
        1 agtattcgaa tcctttaaca catacgccga agccccattg gcaagcgttt caagcacata
       61 agcttcgttg gcatacatcg acaaaatcac aattttggtt tgcggatgtg cttttccaag
      121 ctgacgcgtc acttcgatac cattgagcac cggcatcatt aaatccagga ccagaacatt
      181 gggatgcact ttacgaacca gttcaattgc atcacgtccg ttactggctt ccccaatgac
      241 tcgaaaatca gattcggcct caagcaaggc ccggagcccc tggcgaacaa tatgatgatc
      301 atcagcaagg acaatcgaga tcatacaacg agtacctttc gtgtaaaatc ggtgggttca
      361 agaaggatgg gcaacagaaa acaggttgaa atctttagcc ctgaaaaagt aacttacagg
      421 ggttaactta tttttcgaga atgaattgca gtgattcttg aaccttcaag aattttcagt
      481 tgaagccggt gcgaatacct ctgatttgag tttttggaga atgacgtttc agggcatcca
      541 agatttcagc tttggagctt gagttctgcc gttacccgtg ttccacgccc aggtttggat
      601 tctatggtaa acgttccatc caaaagcagc acccgttcgc gcattccagc cagaccactt
      661 gatactggaa cagagagcac actatcaaca tcaaaacctt tgccacggtc ttcaatttga
      721 atggtaatcg tctggtgatc aacccaggtc cggatggtcg cttcgtcaac ttcggcatga
      781 cgcgcaatgt tggtcagggc ctcctgaaca atccgatagg cagcagtttc aacttccggg
      841 gccaatcgtt tgtcttttaa cccgcgatgg cgaaaagcaa tccggacgtg ggtttgggtt
      901 gtgtagctct caatcaacca gagcaaggca gggagtagtc ccaaatcatc gagcattccc
      961 ggacgcagat cgagcgacaa ttttcgaacc cgtgccatca actcattgac gatttgctgg
     1021 gacagggcaa tgtttttctg ctgggcttca gtggatgcct gccgggcgtt taaatcaagg
     1081 gtgaatttca acccggtgag ggcctggcca atttcgtcgt ggagttcaag ggcgatgcgg
     1141 cggcgttcgg cttcctgaac ttccatcaaa cgacgggaaa gaatttgcaa ccgttcggca
     1201 tattcgcgca aatccgcttc ggtctgtttc cggtggctaa tgtcacggac cacggcaatc
     1261 accatgcgac cagtctcgct ttcaatcgga ctgagcatga tttcagccgg aaattcgctt
     1321 ccatcacgcc gtcgggcaaa gagttctaaa ccagcaccca tatcacgcaa atgcggagac
     1381 cgaatgtagc gtgcccgatg ctcttgatga gactgggtaa accgggccgg catgagcaat
     1441 tcaaccggct gcccagccag atcatctgcc cggtatccaa acatctgttc aacctgggcg
     1501 ttgacgcgtt cgatttgacc gcgatgattg accacgacca tggcatccgg tgctgattca
     1561 aaaatggcgc gcaacatctc ttcactctga ctgagcgcgg tttcggcccg tcggcgctcc
     1621 gcaatttcgc gccgcaattc cacgttggca aacaccaact cctcagtgcg ctcgcgaact
     1681 cgcatttcca actcatcacg cgctttcatc aattccgttt cggcccgttt gcgttcggca
     1741 atttcggatt cgagaaagaa attggcatcg gccagttcgc gggttcggcg gcgaacacgg
     1801 tcttcaaggt cagtatgtgc cgatctgagc gcggcttcgg cttctttcag ttcagtaatt
     1861 tcaaaggcaa cgccgtgaat gtaccagggg cggccatctc tgtggcgcac cattttcact
     1921 tcagcatgaa accagaccac acgcccattg cgcgccagca ctcgatatac cgaacgcaat
     1981 ggatcacccg tcaaaaaggt gtgggcggcc tccatactcc agcgggtttt gtcttccggg
     2041 tggagttgct caaaccagcg cactggatca ttaagccact cagcctgagt aaaccccagc
     2101 aacgactcaa tgtgcgggct gacgtaggct tcgctgaggc ccttgtcaaa gaacgccata
     2161 aagaccacgg ctggaatttg ttcgaccagc acctgatagc gagaggacag gtctggtagt
     2221 tcttcttcag cttcgctgga tttgcgatga aaccctggaa aaaaatcaaa aacagtagct
     2281 ggattttgaa aaggcaggaa gacattggcc agttgaaaga gtgttgattg tgattcaagc
     2341 agcccggcca gcagggattc ctcaacgcca aacttgagca gttcggaaat attctctgag
     2401 ttgagccatt ccagttgggg caacttcatg ggttcttttt ccgggaattg atcgagtaca
     2461 gggttggtgt tccgggggca ggtttacgcc aggccggttc ctgtgtcatc gaacccagcg
     2521 taaatttgcg caacgccgta atcgcccggc cataggttga aaccgcctgg gctgaaagca
     2581 acgatcccgg aggctgattc ataaagctca taaatttgtc aggaaatcct ggcatggtgc
     2641 aaccaataca gatgccgccc acatttggac acccgccaac cccactcatc cagccacgtt
     2701 taccaacatt gcattgtacc accggccccc agcacccctg tttgacaata cactgggcgt
     2761 cgccatattg ctcggcaaac tgaccttgtt cataaaaacc gccccggtca cagcattcgt
     2821 ggagtgaatc tgggaaaagc cattttgggc ggagtgcttc atcaagttca atcatcggtg
     2881 catatccggc ggcctgtctg agcaaataga gcagcacctg catcaagttg tcaggctgag
     2941 tcggacaacc aggaacacaa accacagcca ttccagcctt cgatttccaa tcccagccca
     3001 ggtaatcagg gagccccatg caaccagtcg ggtttcccgg cagggcatgg attccgccat
     3061 aggtggcaca ggtgccaaca gccatcacgg cccaggcgtg cggtaccagc cggtcaatcc
     3121 aatcacaggt caaaattggc tgcccggttt caggatcaca cccaaacccg gcccagtacc
     3181 cggattcttt attggtttca ttcgggatcg agccttcaac aatcaacaaa aacggtcgtg
     3241 agtttccggt tgtggcctcg tgaaacgcct tcaaaaagtc atctccggtc tggtaggcat
     3301 acacctgatt gaaaaagcga atttccggtg ttccaggaat ggccccacgg atgacatcct
     3361 cgatgctggg ctgagtggct gccgtcaagg ccactgattc cccgtcacac cccaggcccg
     3421 cagtaatcca taaaatgtcc agttgttcga ttttgacggc tgaactggtt ggttcggtca
     3481 tggcttcgag cctttcccgc cgtgtccaag catcagcgaa tgatgggttt ccagcacttt
     3541 tccattgccc agatacatgt gcaccccaca cggcatgcaa ggatcaaaac tccgaatcgc
     3601 ccgcataatg tcaattccct taaagcgttc gggcggattt tcttcaaaga gcggggtgtt
     3661 ttggatggca tcttcaaacg gtccgggtgt cccaaattga tcgcgcgggc tggcattcca
     3721 gggtgtgggt ggataggggt ggtaattggc tatttttcca tcccgaatga cggcgtgatg
     3781 cgaaatcata ccgcggaccg cttcgtgaaa accgcacccg atagcctggt ccggtacggt
     3841 aaactcgct
//
LOCUS       DAOQDO010000294         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_16971_length_3842_cov_3.170848, whole genome shotgun sequence.
ACCESSION   DAOQDO010000294 DAOQDO010000000
VERSION     DAOQDO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16971_length_3842_cov_3.170848"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /gene="rpsO"
                     /locus_tag="PKZ53_06040"
     CDS             complement(<1..181)
                     /gene="rpsO"
                     /locus_tag="PKZ53_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNJ40031.1"
                     /translation="MALASELKAKIIAENKTHDSDTGSPEVQIALLTKRIEELTLHFR
                     THVKDNHSRRGLLKLV"
     gene            complement(421..612)
                     /gene="rpmB"
                     /locus_tag="PKZ53_06045"
     CDS             complement(421..612)
                     /gene="rpmB"
                     /locus_tag="PKZ53_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNJ40032.1"
                     /translation="MAKRCDICDKGPMFGNRVSHANNRTKRRFNPNLQRVHVRVGGGN
                     THLRVCTRCIKAGKIVKAA"
     gene            976..2211
                     /locus_tag="PKZ53_06050"
     CDS             976..2211
                     /locus_tag="PKZ53_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNJ40033.1"
                     /translation="MNQRFSLRLFLNLVCLICVATLCACNQAASSSGGGAEVAAKVNS
                     VIIPLSKVDRQIEQELKQQTSQSLSELSPIALASLRMMALDRLITQEVLLQRAQKESI
                     QVSDDDVKQRVQELIQAQGWSQEEYQKTIKELGMTEEEFRNEQRKLLLINKLVDRLKT
                     RIPAPSEKEIADFYNQNPEQFKLGKGVYLSQIVVSPTNDGLKNDAVTEEQAKQKIATI
                     AAQLKAGDDFATVARNYSEHQSALQSGDMGFIPEESLKGFPNGLGQRFFAMREGEVTE
                     PIQIQGAFFIFKVTGKKLDEQELKLDNPDVKRKIAEYIRQQREQVISQALMLSAMSES
                     RIENYLAQRVLENPANFGSTRPASLNASPQPTPATEAKPAETKPTEPAKPAETTKPAE
                     TTKPAETAKPAETKPADKK"
     gene            2423..2587
                     /locus_tag="PKZ53_06055"
     CDS             2423..2587
                     /locus_tag="PKZ53_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016516386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNJ40034.1"
                     /translation="MKVKEIIKIIEADGWFLAKTEGSHRQFKHPLKSGKVTVAGKLSD
                     DLLKKTESSI"
     gene            2628..2933
                     /locus_tag="PKZ53_06060"
     CDS             2628..2933
                     /locus_tag="PKZ53_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ40035.1"
                     /translation="MNQYLIVIEKGERNYSAFSPDVLGCVATGKTIEKTVEEMKSALR
                     LHLSEMLKDGDALPTPKGIEFHLTESQKTEEPFLEAGDYLTYIPIEVVLPDDFSIAA"
     gene            3319..>3842
                     /locus_tag="PKZ53_06065"
     CDS             3319..>3842
                     /locus_tag="PKZ53_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ40036.1"
                     /translation="MLFRLDDVYKSYGATDVLCGVTCQINPGEKVGLVGRNGAGKSTL
                     FKLVCGTETPDRGQVVRINNLSIGLLEQHPNFPSDITVFEAARSVFSTLLKIEADMLD
                     LEHEMAEHSEENEFLEQILEKYSDCQHRFEEQGGFTYRTRIEEVLMGLGFQKSDFDTH
                     VMTLSGGQQGRLHLG"
BASE COUNT         1056 a          925 c          960 g          901 t
ORIGIN      
        1 taacaagttt caacaaacca cgacgggagt ggttatcctt gacgtgagtt ctgaagtgaa
       61 gagtgagttc ttcgatgcgc ttggtgagga gcgcaatttg aacctcgggc gatcccgtgt
      121 cggaatcgtg ggttttattt tccgcaataa ttttcgcttt taattcgctt gcgagtgcca
      181 cagaaaaatc ctccgcagta aacaaacctc tgcccgctgg ggcaggagtt tcagatatca
      241 gaagtgattg aaaaccgcaa aaacgcgtga tgaaaagctg gcttggatga gggtgagaga
      301 agccgtgcgt ttttggattt tacgatcagc gaaagaaacg gaccgtaggc atcgtgtgat
      361 ggcaggagca accatgttcc cgcgtaccag agcttcggct agttcttcaa acaacaagac
      421 ttaggcggct ttgacaattt tgccagcctt gatgcagcgg gtacaaacgc gcaaatgggt
      481 gttaccacca ccaactcgaa cgtggacgcg ctgcaaattt gggttaaagc gccgcttggt
      541 gcggttgttc gcgtgggaaa cgcgattacc aaacatgggc cctttatcgc agatgtcgca
      601 tcgtttcgcc atgggaattg ctctcctttc ttttcttagt tacaatgcaa aactcgactt
      661 tatagcaaaa tcgtcactgg taagtcaaac caagctctta tcttttgaag ccaaaagtct
      721 taaacgcctc atgttcagct attgccgggg acgcgcggca cttatcgccc ggaaacgctc
      781 caggcccgga ccggttgagt cagcacgtcg ttgagacgca cccactgcct gtcgtcactg
      841 gctgaaccca cctcgattca aatgctgaat gatgaaatca ctgactgtcg ggtatcgaaa
      901 ccaatgtaat tggatttgtt cttcatcatt cagcatttac cactctctaa atttctgtgt
      961 aaggagtttt tctacgtgaa ccagcgtttc tctcttcgtt tgtttctcaa ccttgtctgc
     1021 ctgatctgcg tcgccacgct atgcgcctgc aatcaagcag catcaagcag cggcggtggc
     1081 gccgaagttg ccgctaaagt gaattcagtc atcattccgc tttcaaaagt tgatcgccag
     1141 attgaacaag aattaaagca gcagaccagc cagtctctga gcgaactgag cccgattgcc
     1201 ctggcttcgc tgcggatgat ggcactggat cgtttgatta ctcaggaagt gttgctgcag
     1261 cgggcccaga aagaaagcat ccaggtttct gacgacgatg tgaaacaacg cgttcaggaa
     1321 ctgatccagg cacaagggtg gagccaggaa gagtatcaaa aaacgatcaa agaactgggc
     1381 atgacggaag aagaattccg caacgaacag cgcaaattat tgctcatcaa caaactcgtt
     1441 gatcggttaa agacccggat tccagcgcct tccgaaaaag aaattgctga tttctataac
     1501 cagaaccccg agcagttcaa actgggcaaa ggggtgtacc tgagccagat tgtggtgagc
     1561 ccaaccaacg acggcctgaa aaacgacgcc gtgaccgaag aacaggccaa gcagaaaatt
     1621 gcaacgattg ccgcacaact caaagccggt gatgactttg cgacggtggc ccgcaactac
     1681 tctgagcacc agtcagcctt gcagagcggc gatatgggct ttatccctga agaatcgctc
     1741 aaagggttcc ccaatggatt ggggcagcgc tttttcgcca tgcgcgaagg tgaagtcact
     1801 gagccaattc aaatccaggg ggcttttttc attttcaaag tgacgggcaa aaaactggat
     1861 gagcaggaac tcaagctcga caacccggat gtcaaacgca agattgccga atacatccgc
     1921 cagcagcgcg aacaggtcat cagccaggcc ttgatgctga gcgcgatgag cgaatcccgg
     1981 atcgagaact atctggccca gcgcgtgctg gaaaacccgg ccaattttgg ttcaacgcgc
     2041 ccagccagcc tcaacgcttc accacagccg acgccggcca ccgaagccaa accagccgaa
     2101 accaagccaa ccgaaccggc caaaccggct gaaaccacca aacccgccga aaccaccaaa
     2161 cccgccgaaa ccgccaagcc agccgaaacc aagccagccg ataaaaagta aggcaggctg
     2221 gaagaacgtc gggctgaaga gataaatatt tcctgggagc gccggcatct tgccggcatc
     2281 aatcggttga ttttcaatca gttgtctgta ggatgctggc gttcccagag agaatctttt
     2341 tctgaaaact atagttggtt ttttctatta gctttttgta agttttttaa aacattgagt
     2401 tacatcggcc agctacatca aaatgaaagt caaggaaatc attaagatta tcgaagccga
     2461 tggttggttt ttagcaaaaa ccgaaggcag tcaccgccaa tttaaacacc cactcaaatc
     2521 aggaaaagtg actgtggcag gaaaactcag tgacgacttg ctcaaaaaaa ccgagagtag
     2581 catttgaaga caagcaggtt tgaaataaac cttcgatgga gtgtgcgatg aaccaatatc
     2641 tgattgtgat tgagaaagga gagcggaatt actcggcttt ttctcctgat gtgttgggat
     2701 gtgttgccac tggaaaaacg attgaaaaaa cggttgaaga aatgaaatct gcgctccgat
     2761 tgcacctctc ggagatgtta aaagacggtg acgcactgcc gacgcccaaa gggattgaat
     2821 ttcaccttac tgaaagccaa aaaaccgaag aaccattttt agaagctggg gactatttga
     2881 cctatatccc cattgaagtt gtgctgcctg atgatttttc aatcgcagct tgaagctgtg
     2941 attttgtgcg tgaaaaatgt gtcactggtt ctttgggatg aagattcgca agctcatggc
     3001 tgaagaaaac gggctgaaga cattgaggaa gttcaacgtg aattccagag gtcttcagcc
     3061 ctttgttttg ggcaaaaccg tcaggagttc caccttcagg cgggttttga aatatcagtt
     3121 ggtggagtgt gtgatgaacc aatatctgat tgtgattaaa aaaggagagt ggacgtactc
     3181 aaccttgtta tctccctgat taacttgcat ggctggaaaa ctgagaatac aaatgggttg
     3241 caacgcattc caaattcaaa tccaacacca cctggtttca agcagtgaaa gccagcccgt
     3301 ggccaatcaa ttgttctcat gctttttcgt cttgatgatg tctataagtc ctatggtgcc
     3361 accgatgttt tgtgcggtgt gacctgccag attaaccccg gagaaaaggt cggtctcgtt
     3421 gggcgcaatg gtgccgggaa atcaaccctc ttcaaacttg tgtgtgggac agaaactcct
     3481 gaccgtgggc aggttgtccg gatcaacaat ctctcaatcg gcttactcga acaacatccc
     3541 aacttcccat ctgatatcac ggtttttgaa gccgcccgta gtgttttctc cacgcttctc
     3601 aaaattgaag ccgatatgct cgacctggag catgaaatgg ctgagcactc ggaagaaaat
     3661 gagttcctgg aacaaatcct ggagaaatac agcgactgcc agcatcggtt tgaagagcag
     3721 ggtggtttta cctaccggac ccgcatcgaa gaagtcctga tggggcttgg ctttcagaaa
     3781 agtgacttcg acacccatgt catgacgctc agcggtggcc agcagggccg gttgcacctg
     3841 gg
//
LOCUS       DAOQDO010000295         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17010_length_3837_cov_2.684558, whole genome shotgun sequence.
ACCESSION   DAOQDO010000295 DAOQDO010000000
VERSION     DAOQDO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17010_length_3837_cov_2.684558"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2601)
                     /locus_tag="PKZ53_06070"
     CDS             complement(106..2601)
                     /locus_tag="PKZ53_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40037.1"
                     /translation="MRIHETENLPSLRKMEVESDYCEPLDLKTFQRDLHAAHRIVLSR
                     LVAYIEHFEQVLGLNTVFRFMNVALQEGLLISQPEAETIFENISKLYFQHPHYSKFVP
                     SRFIGRRKEIQRAITWAAGHQAILWVCGFGGNGKTIFQMFLAQSLRCGWDHLRSEFQK
                     CEPYEATIWMADSEASPLDFDSFLNKITFVLDNPSHHLYLEGNQDQLPPSPTSQNRKS
                     DPEDVRQLLQKRRALVVVDNAETIEPVELTKILGFFKLLPGESRSRLVIGARSEPKLS
                     ESEGITSILIKGLEPQEASQFVTDLCHSKMVSLSEDEIQAICVETECNPAALQIIVGL
                     VSRGHELRDILGYLKRGIHREVVTDIYHRIIRFSFIKALSTPERLLLLAKGLFPFAER
                     ETVLADVAGVEREKGHNLFEKLQSLYLVNFEKLATKPGAPEKWMIDTHNLIQRFLETI
                     WHGEDYGIEELKTLKPEIEKRWWSYAYNLNERVGQYNFEQIEQREGFPKKVGNVLGQI
                     SKRIERDELKALELFTCYEDGYGHIFLQWCEWHDVLRICKNAVPLALRSHKPKLLGYL
                     LLDQMSTIHRERGDFKECWACIKEAEKYHSELYAVMSSIADPNARELFKAELDWFHAD
                     TKMVRGNIYRREGNYVPAIGAYQETEKLFHQLGDVRKSLDVTINHGGCLLEQALENLR
                     DHDGNPVPVDSQGIMSERLSLIKDLFHSAIKKANESGLDSTRWWKFSTGAACIAWLGV
                     LARVNGDYSTAQQLLKESESGFHRQLSIARLYREQALVEHLLGNKKAARDLDMRGIQL
                     LEKAQVFHSREFSHQNYHRVIESLKNSNKWCTD"
     gene            2995..>3837
                     /locus_tag="PKZ53_06075"
     CDS             2995..>3837
                     /locus_tag="PKZ53_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40038.1"
                     /translation="MKKNLRSLFPILVFSTLFVGSIQAVFAQNTAKKDSKPKKSRVNL
                     KAASLPLITETPVVYKASKSQKGQFFRIEVPQNQFVRLQVNQIGIDVKVEVRGPDNRL
                     IQEMDSLTGYGGVEEIQFVSQLSGIHRVEIIFLPPLPPQSQEMIEIWRDPFRVPHEQD
                     RETAKAHDLLYSGLGALLSGDFNQARGKLEQALAIFSRYQCTEREAYTHFLLGQVTLE
                     LALATNPDPTNKTKVVEEIADESLNHFTQASDLWIKADPEKNKRNIALSLSGLAKIQS
                     GLNKY"
BASE COUNT         1079 a          838 c          820 g         1100 t
ORIGIN      
        1 taaaaggaga atttcaatag ctgggatgct tgatagtcac ggggtggctg agaagaagag
       61 caaaaccaca ggtacccgtt ttatatactg ctgcgtattg aattcttaat cagtgcacca
      121 cttgtttgaa ttcttcaagg attcaatcac acgatgataa ttctgatgtg agaactcgcg
      181 tgagtggaag acctgtgctt tttccagtaa ctgaatcccc ctcatatcca agtccctggc
      241 tgctttctta tttccaagta aatgctccac aagtgcttgc tctcgataaa gtcttgctat
      301 tgaaagttgt cggtgaaatc cggactcact ttccttcagc aactgttgag cggttgaata
      361 atcaccattc acacgcgcca gcacaccaag ccaggcaata caggccgcgc ctgtagaaaa
      421 cttccaccaa cgggttgagt caagtccaga ctcattcgcc ttttttatcg ctgagtgaaa
      481 cagatcttta atcaagctca gccgttcact cattatgcct tgagaatcaa caggtactgg
      541 gtttccatcg tgatcacgta aattttccag tgcttgttca agaagacatc caccatggtt
      601 aatcgtcaca tccagcgatt tcctcacgtc accaagttga tgaaataatt tttctgtttc
      661 ctggtaagca ccaatagctg gaacatagtt tccttccctt cgataaatgt ttccacgaac
      721 catcttcgtg tcggcgtgaa accagtccag ctctgctttg aaaagttcgc gagcattcgg
      781 atcagcaatt gaactcataa cagcatagag ttccgaatga tatttctcag cttctttgat
      841 acatgcccaa cattccttga aatcaccccg ttcacggtgg atcgttgaca tctgatccag
      901 taataaatag ccgagcaatt taggtttgtg agatctgaga gccagaggga ccgcattctt
      961 acaaattctc aacacatcat gccactcaca ccactgcaaa aaaatgtgtc catatccgtc
     1021 ttcataacat gtaaatagct caagtgcctt gagttcatct ctctcaattc gttttgagat
     1081 ttgaccaagg acatttccaa cttttttggg gaatccctcc cgttgctcta tctgttcaaa
     1141 attgtattgt ccgactcgtt cattcaagtt gtacgcataa ctccaccaac gtttttcaat
     1201 ctccggtttc aatgttttga gttcttcgat tccatagtct tcaccgtgcc aaatggtttc
     1261 aagaaatctt tgaatgagat tgtgagtatc aatcatccac ttttcaggtg ctccaggttt
     1321 tgtggcgagt ttttcaaagt tcacaaggta gagtgattgt aacttctcaa agaggttgtg
     1381 tcctttctcc ctttcgaccc cagctacatc ggccaaaaca gtttctcgct cagcaaaggg
     1441 aaacagccct ttggcaagca gaagtaatct ttcaggcgta gacagagctt taataaatga
     1501 aaagcggatg attcggtgat aaatatcggt aacgacttcg cgatggattc ctctcttgag
     1561 gtatcccaga atatcacgaa gttcatgacc tcggctgacc aatccaacga tgatttggag
     1621 agcggctgga ttacattcgg tttcaacaca gatagcctga atttcatctt cggagaggga
     1681 aaccattttt gagtggcaca gatctgtgac aaattgagat gcttcctggg gttcaagtcc
     1741 tttgataaga atcgaggtga tgccttcaga ttcagagagt ttcggttcag agcgggcgcc
     1801 aataacaagc cgactacggc tttcgcctgg cagcaatttg aaaaatccaa gaatctttgt
     1861 caattcgacc ggttcgatgg tttctgcatt atctacaaca accagcgctc gtcgtttttg
     1921 aagcagttgg cgaacatctt ctgggtccga ctttcggttt tgggaggttg gagagggagg
     1981 gagttgatct tgatttcctt cgaggtacaa atggtgactg ggattgtcaa gtacgaaggt
     2041 tatcttgtta agaaaagaat cgaaatcaag tggtgaggct tcactgtcag ccatccaaat
     2101 tgttgcttca tagggttcac atttctggaa ttcggagcgg aggtgatccc acccacaacg
     2161 caaggattgg gccaggaaca tttgaaaaat ggttttcccg tttccgccaa atccgcaaac
     2221 ccacaaaata gcctgatgtc ctgccgccca ggtgatcgcc ctttgaattt cttttctgcg
     2281 accaatgaag cgtgacggaa caaacttcga gtaatgtggg tgttgaaagt acagctttga
     2341 aatgttttcg aagatagtct ccgcttcagg ttgactgatt aacagtcctt cctgaagggc
     2401 tacattcata aatcgaaaga cagtgttgag accaagaact tgctcgaaat gttcgatata
     2461 agcaacaagt cgtgacagta ctatccggtg ggcagcgtgt aaatctctct gaaatgtttt
     2521 taaatcaagg ggttcacagt aatcgctttc cacctccatc ttgcggagac ttggtaaatt
     2581 ctcggtttcg tgtattcgca tcaagtactt atcaggtggt aacgcagtga gccctctctg
     2641 tagtcgggcc ttgttgacca tctcacgaag caattgttta gagtttggga acataattga
     2701 tatacttcac agctcaaaat caatgtgcaa aaattctgat tcatcaattg gacattttaa
     2761 gacattttta gcccaaaagg gtaacttttc tggacacgga atcacacaca ttcctcaaga
     2821 aagtcatcta tctataataa attcactaaa ctgatgtcgt tgtaatgtct ctgcaggtat
     2881 ttcagtatag cagctcacat aaaaaagttt ctgagttcaa aatgccttct gatccagatc
     2941 aggattgggg ttaagaactg aagaaccgaa acttttaacc aggaggtaca gcagatgaag
     3001 aaaaacctac gctctctttt tccaatactg gtgttttcaa cgttgtttgt tggatcgatc
     3061 caagctgtat ttgctcaaaa caccgcgaag aaagattcaa agccaaagaa aagccgtgta
     3121 aatctcaagg ccgcctcttt gccgctgatt actgaaaccc cagttgttta taaggccagt
     3181 aaaagtcaga aaggccagtt ttttcgaatt gaggttcctc aaaatcaatt tgtgaggctg
     3241 caagtcaatc aaattggcat tgatgtaaaa gttgaggttc gggggcctga taatagattg
     3301 atccaagaaa tggatagcct gacgggctat ggtggagtcg aagagataca gttcgtctca
     3361 caactctcgg gcattcatag ggtagagatc atttttttac ccccgttacc tcctcaaagc
     3421 caggaaatga tcgaaatttg gcgagatcca tttcgagttc ctcatgaaca agatcgtgaa
     3481 actgccaaag cacacgatct tttgtactca gggttgggtg ctctacttag cggagatttc
     3541 aatcaagcac ggggtaaact tgaacaagct ctggccattt tttccaggta tcaatgtact
     3601 gaaagagaag cctatactca ctttcttttg ggtcaggtta cattagaatt agctctggcg
     3661 accaatccag atccgaccaa taaaacgaag gtggtagaag aaatcgcaga cgaatcgctc
     3721 aatcatttta ctcaggcttc tgatttgtgg ataaaagctg atccagaaaa aaacaaacgt
     3781 aatattgctt tgagtttaag cgggttagcc aagattcaat caggtcttaa caagtac
//
LOCUS       DAOQDO010000296         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17081_length_3826_cov_3.334924, whole genome shotgun sequence.
ACCESSION   DAOQDO010000296 DAOQDO010000000
VERSION     DAOQDO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17081_length_3826_cov_3.334924"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKZ53_06080"
     CDS             <1..275
                     /locus_tag="PKZ53_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartoacylase"
                     /protein_id="HNJ40039.1"
                     /translation="KQGNDGFFTVREIKPFWLKVSNTLRKARADRLLPWLPGVSTHPT
                     QPETYVLDQRITRWYVIEICHLLGYRIHSEHTEHSLTVSRRKQADA"
     gene            484..3126
                     /locus_tag="PKZ53_06085"
     CDS             484..3126
                     /locus_tag="PKZ53_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNJ40040.1"
                     /translation="MSENKDASVLSSQLKAWFLGPKAENEDFFEKLILDAFRDYCYWR
                     RNFHPEDEPGIRASDRRDADFEEFRELIHDRLFEMVSHLKRSAPGFSPRYLGHMMSDL
                     LIPGVVGYVATMLYNQNNIYSEASTITLQFESEALKLIARMLHLDPDTSAWGHLCSGG
                     TGANLESMWIARSVRFFPYQLALALENARGRGQFSTNETVAATISRLGVGKRCSFEEL
                     VGRDQLANLRISEIIELRKQLSDAAGQHHELGEYIKPFLGLGLIELSNQCHKVNTFTF
                     PPSFKVLLSTNAHYSLRKSLGIVGLGESNLVKVKLDSQMRLDSDLLHDQIHRALHHKD
                     VILGVVGVYGSTEEGAIDDFAQIVALRELVRQEYNTDFWLHGDACYGGYALAMSHPFS
                     GSLQQSTEDITTFMWELLKSQVRGEIPNHVAWTKTQSQEWTRHSLALGQCDSVSIDPH
                     KLGYIPYPAGAVFYRDYRVREFIRYDAPYLNISAEKGSDSTTRVEKGGKHWEIGELGK
                     YTLEGSRPGASGAAIWLAHKTVPLDRDGHGRIVAQSVLGARYLQATLEKELQTDSPSG
                     IGCAFLCENPDLNILCYTFPSIIRFPELNNLEIPSSLAVLNRAVTRFFTECLPTEERP
                     TLTREFVVSMTSLSEEEYGGTLGNFMDRLHQQGMIGQLVEELPNGTLTGNPWRDDSKI
                     SVIRTVVMSPFLLKAMTRPKMRASRRELVHEYADFLQTTLGRIFKAELGKAIEKANRP
                     VLTGNVMVIENTEETLVGLCDELRRVSFQADPRQVVGMKTIDLAWEFIPNSDVVLIDS
                     KLAQQDSTRGFQFLEKLRDTEHIKGLVVFASDPLVKERILEGACRRPDWTVTVRQKPD
                     PLSGQFQAAANLVMADVWDCLAKK"
     gene            3263..3766
                     /locus_tag="PKZ53_06090"
     CDS             3263..3766
                     /locus_tag="PKZ53_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40041.1"
                     /translation="MDKIELWLSLSQEVQQLIQRHITEDEFVTLGFILNERYATLPST
                     HHLAHYLFDLEPTDTPIVVCYQIHQALAASLKREYQRAYEGNFGEETEWWKQLRESRY
                     ASLLKMISLTQEFVERALLFNESTLPLVGVPPFALPRWISDNLHLYQQLNTPDPDEDA
                     EHQVALR"
BASE COUNT          958 a          975 c          924 g          969 t
ORIGIN      
        1 aaaaacaggg caacgacggc tttttcaccg ttcgcgaaat caaacccttt tggctgaaag
       61 tatccaacac cctgcgcaaa gcaagagctg accgcctgtt gccgtggctc cccggagtca
      121 gcacccaccc cacccaacct gaaacctatg tccttgacca gcggattacc cgctggtacg
      181 tgatcgaaat ttgtcatttg cttggctatc gcattcattc tgaacacact gaacactcac
      241 tcaccgtcag tcggcggaag caggctgacg cttaagtgga ccacgagccg tcaatcccca
      301 agaacaagtg gttaaaaaat aaaaggtctc caaaccttca acatcgcagc ctcttcaaaa
      361 ccagatgggt tgcttttaaa gtgactcatc ctgttttttt cagaatattc attgattgac
      421 gactcgcgac tcaccactta ctttttgtgt gctccttcac tccttcacag cctcacaaat
      481 catatgtctg aaaacaaaga tgcctctgtc ctctcgtcgc aactcaaagc ctggttttta
      541 gggcccaaag cggaaaatga agattttttt gaaaagctca tcctcgatgc ttttcgtgat
      601 tactgctact ggcggcgaaa ttttcacccg gaagacgagc ctggtattcg agccagcgac
      661 cggcgcgatg cagatttcga agagtttcgc gaattgatcc acgaccgtct gtttgaaatg
      721 gtgtcgcatt taaaacggag cgctccgggg tttagccctc gatacctggg tcatatgatg
      781 agtgatttgt taattccagg cgtggttggg tacgtggcca ccatgttgta caaccagaac
      841 aacatctatt cggaagcctc aacgattacc cttcaatttg agtcagaagc gttaaaactc
      901 attgcccgaa tgttgcacct ggatccagac acgtccgcct ggggacattt gtgttcgggt
      961 gggactggcg ccaatcttga atcaatgtgg attgcccgca gcgtccggtt ttttccctat
     1021 caactggcgc tggcgcttga aaatgcgagg ggacgtggtc agttttctac caatgagacg
     1081 gtggcagcga caatttcccg attgggagtt ggaaaacgct gttcgtttga agaactggtc
     1141 gggcgcgacc agcttgccaa tttgcggatc agtgaaatta tcgaacttcg aaaacagctt
     1201 tcggatgccg ccggacaaca ccatgaactt ggcgagtata tcaaaccctt tttgggactg
     1261 gggctgattg aactgtcaaa ccagtgccac aaagtcaaca cctttacatt tccaccaagt
     1321 tttaaagttt tgctcagcac caacgcccat tattcgcttc gcaagagtct tggaatcgtt
     1381 gggttgggag agtccaatct ggtcaaagtc aaactcgatt cgcagatgcg cctggattct
     1441 gacctgttgc acgaccagat ccatcgggcg ctccatcaca aggatgttat tttaggtgtc
     1501 gtcggcgtct atggctcaac cgaagaaggg gccattgatg attttgccca aattgttgca
     1561 ttgagagaac ttgtccgtca ggaatacaac actgatttct ggcttcacgg tgatgcctgt
     1621 tatggggggt atgccctggc catgtcgcat ccattttcgg gttcactcca acaatccacc
     1681 gaagatatca ccacctttat gtgggaattg ttgaaatcac aggtgcgcgg cgaaatcccc
     1741 aaccacgtcg cctggaccaa aacacaaagc caggaatgga cccggcattc gctcgcgtta
     1801 ggacagtgtg attcggtctc aattgacccg cacaaactgg ggtatatccc ctacccggca
     1861 ggagctgtat tttaccggga ttatcgggtg cgcgaattta ttcgttatga cgcaccatat
     1921 ctcaatattt cggctgaaaa gggatcagat tcaaccacca gggttgaaaa aggcgggaaa
     1981 cattgggaaa ttggcgaact tggaaaatac acgcttgagg gttcgcgccc tggagcgtcg
     2041 ggggccgcca tctggctcgc gcataagacg gttccgttgg atcgcgacgg ccatggccga
     2101 attgtggctc aatcggtcct cggtgccagg tacttgcagg caaccctcga aaaagaatta
     2161 cagactgatt caccttcagg tattgggtgc gcgtttttgt gtgaaaaccc tgatttgaac
     2221 attttgtgct acacctttcc gtcaattatt cgatttccgg aattaaacaa tcttgaaatc
     2281 ccttcgtccc tggcagtgct caatcgggca gtgacacgct tttttaccga gtgtctgcca
     2341 actgaagagc gccccacctt gacccgtgaa tttgtcgtgt caatgaccag cctcagcgag
     2401 gaagaatatg gcggcaccct gggaaatttc atggaccgtc ttcaccaaca aggcatgatt
     2461 ggccagcttg ttgaagaact cccaaatggc acattgaccg gcaacccatg gcgggatgat
     2521 tctaaaattt cagtcattcg gactgtcgtg atgagtccat ttttgctcaa ggccatgacc
     2581 cgaccgaaaa tgcgggcgtc acgccgcgaa ctcgttcatg aatatgccga tttccttcag
     2641 accacattgg gccgaatctt caaagccgaa ctgggtaaag ccattgaaaa ggcaaatcgt
     2701 ccggtgctga ctggaaatgt aatggtgatt gaaaacaccg aagaaacact ggttggtctg
     2761 tgtgatgaac tgcgacgcgt gagctttcaa gccgacccgc gtcaggttgt gggaatgaag
     2821 accattgatc tggcctggga atttattccg aattcagatg tggtattgat cgatagcaaa
     2881 ctggcgcagc aggattcaac cagagggttc cagtttctag agaaactccg tgacacagag
     2941 catattaaag gactggttgt ctttgcctca gacccacttg tcaaagagcg aattttagag
     3001 ggtgcttgcc gccgtcctga ctggacagtc acagtccgtc aaaaaccgga cccgctcagc
     3061 ggacaatttc aagccgccgc caatctggtc atggcagacg tttgggattg tctggccaaa
     3121 aaataatcgg cggcaccgtc caggtctcct ttatttcgcc agtccatcca gctttcaaat
     3181 taaaccaggc actcaatttt gcccattgtc agccggcaga tttggactat gcttgtgaga
     3241 cgtaaccaaa ggagaccagt gcatggataa aatcgagttg tggttgagtc tgagtcagga
     3301 agtccagcaa ctgattcaac gccacattac tgaggatgaa tttgtaacgc ttgggtttat
     3361 cctgaatgaa cggtacgcca cattgccgtc aacgcatcat ctggcacact atctctttga
     3421 cctggaacca acggatacac caattgtggt gtgctatcag attcatcagg cgctggctgc
     3481 gtcactgaaa cgggaatatc agcgggcgta tgaaggaaac tttggcgaag aaaccgaatg
     3541 gtggaagcaa ctccgggaaa gtcggtacgc cagtttgctc aagatgattt ccctcaccca
     3601 ggaatttgtt gaacgagcac tcctgttcaa tgagtccacc ttgccgctgg ttggtgtccc
     3661 accttttgcc ctcccgcgat ggatttccga caatctccat ctgtaccaac aactcaacac
     3721 gcctgatcca gacgaagatg ccgaacatca ggttgccctt cgttaacggc tgtttctcaa
     3781 ttcttcattc tcaattcttc attcttcatt ctcaattctt cattct
//
LOCUS       DAOQDO010000297         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17099_length_3824_cov_3.161847, whole genome shotgun sequence.
ACCESSION   DAOQDO010000297 DAOQDO010000000
VERSION     DAOQDO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17099_length_3824_cov_3.161847"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKZ53_06095"
     CDS             <1..215
                     /locus_tag="PKZ53_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40042.1"
                     /translation="DVVAKAAVELGKDFFRPKLLCPLEFMKKRHELGFKGGLFQVPEF
                     KEKDTCHKAPVNPAGALDFANQRTVR"
     gene            complement(273..476)
                     /locus_tag="PKZ53_06100"
     CDS             complement(273..476)
                     /locus_tag="PKZ53_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40043.1"
                     /translation="MTVLDPQRGYLFQPWVSVSATHGGEHPADPNPERVDGQPDHPPK
                     RVPVGQRQRHPRLFGSIACRDAD"
     gene            complement(538..3534)
                     /locus_tag="PKZ53_06105"
     CDS             complement(538..3534)
                     /locus_tag="PKZ53_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ40044.1"
                     /translation="MAFWIKTVLAACVCLIGFHFFNLPVLAQSGGATTGEIIGRVTDT
                     QGNNLGGVSILARQFETGFTRQIQTAESGEYALLRLPPGQYEIKAESDGFATKTSRLT
                     LAIGTTGLVIFQLTPAGTNEVIEVRASDADFLGEKTESSSVINRQQIDGLPINQRNFL
                     DFAQTTARAVPDPIGGQGVLATSGISFNGQLARFNNVSIDGLDNNDRVSGAVRTTFSQ
                     EAVQEFQVVSDGFSAEFGRATGGIINIITRTGSNEFHGNLFYFNRNDTISARDAFTAT
                     KAPFSQYQYGATFGGPLRRDRSSFFTSFERLNIQQNNIVTIPDGIVSAAAGAGYAFQN
                     GPIPFSVGRTALLARVDTQLSSQDTFWVRYNGGFASDGSYERFGGLIAQSSGGLLDLN
                     EHTLAVGNLYASSAHNYVNETRFLYSHRDQSIEPLDRSPHVQSSSLAGLVEFGRSTLL
                     PNTRKEHIFHLADTVTLTRGNQTLKFGGDLQFFHQPEATIPLLVGGLMRFTGLDLAAL
                     IGNRNLPFITELQTLDPRLRTPAQILALQQLSQTLPQTLPNFPKNVPLDRLPLPLVYI
                     QSFGNPNVSGLGTRSASAFAEDEIKVTPSFLVRLGARYDWNQVLGIDATNGNFSPRIG
                     LSWQPTRLPQVTLRAGYGIFVIGPLAANAGAAKLAADKRLDVQGIPFPFSVLPLATPE
                     RRIPEGATPPPDRPFIPQLGTIATFQPDLKNSYSHQANLTLTYALTTTLNLSAGYSFV
                     RGIRQSSVRNINPVVRPRPNQLEAFIDGRVDPTQGSILETESAFDSYYHGVTLGLEKR
                     FASQFSFLSHYTFSKSIDNFDDIFIGENETNDPLQPGLERGLSSQDARHRLVISGLWN
                     LDYTTNPILKDFQLSGILNLKSGSPYNLLSAVDINGNGDFPIGDRPPGIARNAGILPG
                     FAGFDLRLTRKFSIGERVQIEALVESFNLFNRTNISEINRIFQPNPERMFILPSQENG
                     RYIAPPERFRNAFAPRQFQFGIRVNY"
     gene            complement(3568..>3824)
                     /locus_tag="PKZ53_06110"
     CDS             complement(3568..>3824)
                     /locus_tag="PKZ53_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40045.1"
                     /translation="PGSPVTGTTRQSVHNLGGAVEYIGGAFALWKISEYLGQPFQIAA
                     FIVGAAAILLSVPETGAVRGLIQRLAEACLFGGLVWSAAA"
BASE COUNT          933 a          913 c         1080 g          898 t
ORIGIN      
        1 gtgacgtggt ggcgaaggcg gcggttgagc tcggcaaaga cttttttcgg cccaagttgt
       61 tgtgcccgct ggagtttatg aagaaaagac atgagttggg tttcaaaggt ggtttatttc
      121 aagtacctga gttcaaagag aaggatacat gccacaaagc tccggtcaat cctgccggag
      181 ctttggattt tgccaatcaa aggacagtta gatagatcaa tactcatttc ttatcgttcg
      241 ctcaaatttg ggatgcttcc gaaacattga atctaatccg catcccggca cgcgatactt
      301 ccaaacagcc gtgggtggcg ttgacgctga cccacgggaa ctcgttttgg tgggtggtcc
      361 ggttggccat caacccgttc cgggttggga tcggcgggat gttctccgcc gtgggtggcg
      421 ctgacgctga cccacggctg aaacaggtat ccccgttggg gatcaagcac cgtcattttt
      481 caaaaaccat tcaaaatttg agcgaacagc cagtcatttg tagtcatacc ttgtttctca
      541 gtaattgact ctgattccaa actgaaattg ccggggggca aaggcattgc gaaaccgttc
      601 aggcggggca atataacgac cattttcctg cgatggcagg atgaacattc gctccggatt
      661 tggttggaag atccggttga tttcgctgat gttggtgcga ttaaacaggt tgaacgattc
      721 gaccagcgct tcgatttgaa cccgctcacc aattgagaat tttcgggtca gtcgcagatc
      781 aaatccagca aatccgggca aaatccctgc atttcgggca atgccaggcg gacggtcacc
      841 gattggaaaa tcaccattgc cgttgatatc aacggctgac agcaagttgt aaggtgagcc
      901 ggatttgaga tttaaaattc cggacagttg aaaatccttc aatatcgggt tggtggtgta
      961 atccaggttc cacagtccac tgatcaccag gcggtggcgg gcatcctggg atgacaaccc
     1021 gcgttccagc cccggttgga gcggatcatt ggtttcattc tcgccaatga agatatcgtc
     1081 gaaattatca atagattttg aaaatgtgta atgactcagg aagctgaatt gactggcaaa
     1141 ccgcttttcg aggccaagtg tgaccccgtg atagtagcta tcaaacgccg attcggtttc
     1201 caggatgctt ccctgggttg gatcaacacg gccatcaata aaggcttcaa gctggtttgg
     1261 tcgtgggcga accaccgggt tgatatttcg aactgagctt tgcctgatcc ctcgcacaaa
     1321 actatatccg gcagacagat tgagtgtggt ggtgagggca taggtcaagg tgagattggc
     1381 ctggtgggag tagctattct tcaggtcagg ctgaaaggtc gcgatggttc caagttgtgg
     1441 aataaatggc cgatccggcg gaggtgtcgc accttcagga attcgccgct ctggggttgc
     1501 cagtggtaaa accgagaacg ggaagggaat gccttgaaca tcaagccgtt tgtccgccgc
     1561 cagtttcgct gccccggcat tggctgccag cggtccaatc acaaagattc catatccggc
     1621 ccgaagtgtg acttgaggaa gccgagtcgg ttgccaggaa agcccaattc gaggactgaa
     1681 attgccgttg gtggcgtcaa ttccgagaac ctgattccaa tcatagcggg cgccaagccg
     1741 caccaaaaat gatggtgtga ctttgatttc gtcttcggca aaagccgatg ccgaacgggt
     1801 tccaagcccg ctcacattgg gattcccaaa cgactggatg tacaccagcg ggagcgggag
     1861 tcggtcaagc gggacatttt tgggaaagtt tggaagggtt tgcggaagcg tttgtgacaa
     1921 ttgctggaga gccaggatct gggccggagt tcgtaagcgt ggatccagtg tttgaagttc
     1981 agtgataaat ggcaggtttc gatttccaat caaggcggct aagtccagac cggtaaagcg
     2041 catcaaccca ccgaccagca acggaattgt ggcttcgggt tggtgaaaaa actgaaggtc
     2101 tccgccaaat ttcagggtct gatttccacg cgtcagtgtg accgtgtctg ccagatggaa
     2161 aatgtgttct tttcgggtat tggggagcaa ggtactgcgg ccaaattcaa ccagtccagc
     2221 caggctggaa gattggacgt gaggactccg gtcaagtggt tcaatggatt gatcgcgatg
     2281 gctatagaga aatcgggttt cattgacgta gttatgggcg ctactggcat agagatttcc
     2341 gacggcaagg gtgtgttcat tcaaatcgag caggccgccg ctggattggg caatcagtcc
     2401 gccaaagcgt tcataggaac catctgatgc aaatccgccg ttgtagcgaa cccaaaaagt
     2461 atcctgcgat gaaagctggg tatccacccg tgcgagcagg gctgtccggc caactgaaaa
     2521 gggaatcggg ccattttgaa atgcatatcc agcacctgca gcggcgctca cgattccatc
     2581 gggaatggtg acaatgttgt tttgctggat attgagtcgc tcaaaagaag tgaaaaatga
     2641 tgaccgatcc cggcgaagtg gcccgccaaa ggtggcccca tattgatatt gagaaaaagg
     2701 cgctttggtt gccgtgaagg catcgcgggc actgatggtg tcgttgcggt taaagtaaaa
     2761 cagattgccg tgaaactcat tgctcccggt gcgggtgata atgttgataa tcccaccggt
     2821 ggcgcgtcca aactcagcgg aaaaaccatc ggaaaccacc tgaaactcct gaacggcttc
     2881 ctggctaaag gtggttcgca cggcaccgga aacccggtca ttgttatcga gtccatcaat
     2941 tgaaacgttg ttgaagcgtg ccagttgacc gttgaacgaa atccccgagg tggcgagcac
     3001 tccctggcct ccgattgggt cagggactgc ccgggcagtg gtctgggcaa agtcgagaaa
     3061 attccgctga ttgattggca ggccatcaat ttgttgacga ttgatcacac tgctgctttc
     3121 ggttttttcg ccaagaaagt cagcatcact ggctcgaact tcaatcactt cgttcgttcc
     3181 ggctggtgtc agttgaaaga tcaccagacc ggtagttcca attgccagcg taagtcgcga
     3241 agttttggtg gcaaacccat cgctttcagc tttgatttca tattgaccgg gcggcagtcg
     3301 caacagggcg tattccccac tttctgccgt ctgaatctgt cgagtgaacc cggtttcaaa
     3361 ctggcgagcc agaatcgaga cgccacccag gttgtttccc tgggtatcgg tgactctgcc
     3421 aatgatttct ccggttgtgg cgccgcctga ttgggcaagc accggcaggt tgaagaagtg
     3481 aaatccgatc aaacacacac aggcggcaag aacggttttg atccagaaag ccatagtcat
     3541 cctttgaaga agtgaaagaa agcagcgtca agccgccgca ctccacacca gtccgccaaa
     3601 gaggcacgct tcggcaagac gctggataag tccgcgcacg gcgccggttt cgggaactga
     3661 tagcaaaatg gcagcggcgc cgacaatgaa agccgctatt tggaatggct ggccaaggta
     3721 ttcactaatt ttccataagg caaaggcacc accaatgtat tcgacggcgc ctccgagatt
     3781 atgaaccgac tgacgcgttg ttcccgttac gggcgaccca gggt
//
LOCUS       DAOQDO010000298         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17111_length_3822_cov_3.767720, whole genome shotgun sequence.
ACCESSION   DAOQDO010000298 DAOQDO010000000
VERSION     DAOQDO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17111_length_3822_cov_3.767720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PKZ53_06115"
     CDS             complement(<1..515)
                     /locus_tag="PKZ53_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNJ40046.1"
                     /translation="MQFDYAYRGNSGVDNQANRTALSFAPDLKREPTFFVAELRKNIA
                     FREAISALHDVVVSDLRFKPKDKTAYKEWAAQREEVDWQQVTSMRKEVAEKIKVLQTE
                     MNELNQRSTNRWGPYWQAQRRFFDYLYQKERDVWFVLDPVITVHPDEVFFECFSQDES
                     SYGRLGVNYDV"
     gene            complement(505..3756)
                     /locus_tag="PKZ53_06120"
     CDS             complement(505..3756)
                     /locus_tag="PKZ53_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HNJ40047.1"
                     /translation="MKLIKQTLLSFREGSSDKVYEVDLCEVSQNRYVVNYRYGRRGTT
                     LKEGTQTVSPVSLADGERIFADLVKSKTKKGYREGASPEPAATEPAAKPTTPLGPPNQ
                     EARNQQILHLLAHPELAQRKPVTTGVLGAVRSVLTGKRQPAKKGWPLERVIWRAGELK
                     LRDAAPLLIRLIGTGDALRDYCIAWALGWCGDEKTVSTLGRIYGDAATPEHVRRIACE
                     ALIKLSDPETRAEFQSDMVDRLPSPLKETARTGKPEEFTSALRGYLESGGPSHYSVLD
                     TIYLIDSQTVRPALLGLLKSAPLRPNYFRAFRHIFKAAEYRRDAEVFGILAYRFEKEK
                     PMFRKLGSYSSYYIDDSSQTHSTEDVKKTLKSASSKLAYSQNTRNFLRIRVWRTLRRL
                     GELGDHDFVKMAVGVLLPYSDADAHPTRTSTRYVARPTTNQRGRHSVDYVAVTNHWDT
                     FAPYFAFNAILYHHSPRYIRPKGSMVWRCKPNARPGQPVPTVREEAFPKLWETMPVGL
                     LHLLSESNCLPVHQFAVRALRACTEFCGQLPVDAVVMLLNRPYEITAQFGFELANVRY
                     TPEVPDFELVLAVASCAYGAARQQAHQWITTSRDRFVKDSGFLAGLASSLYADTRVFA
                     RTLLRSVSFTEDAARACIVKLIAHLLVLEPHQAEQAKDIAETVFTVFSAQLRTIGLNV
                     VADLVTHPMVEVQELGGNILLNHETKAHNLPESLIHALIQSNFETIRAIGIRLLSELP
                     DELLVNRKGVLRAICIHALPDLRQAIRPLIRRLGAAETHPDFILGFSTLLLESLLKPQ
                     PNDGVYSHLVLVLKEDLNPMWGRQVSKELVWRLIKSRASAAQDVGGYLLKLNVAWAEE
                     LTTDDITRLCNHEMLSVREAVQALFQAIVPRLRGNHEEMGKAIRLLDSKWPDLRQFGV
                     KILREEFSESDFNPQILVSLCDSIRPDIQQFGREMITRYFTEADGQEYLLKLSEHPSA
                     TLQMFATNYLEGYAAGNVERLEQLTLYFVTVLSHVNKARVAKTRILQFLEAEAIKSEA
                     AAQLVAEVMSRQSVTMAIGDKASTIEIMVRLHQKYPSIPLPMAVKPAEVRYAV"
BASE COUNT          915 a          997 c          996 g          914 t
ORIGIN      
        1 aagacatcat agttgacgcc cagccgacca tagcttgatt catcctggct aaaacactca
       61 aaaaacactt cgtcgggatg cacggtgatg accggatcca gaacaaacca gacatcgcgt
      121 tctttttgat acagatagtc aaagaaccgg cgttgcgcct gccagtacgg gccccagcgg
      181 ttggtactgc gctggttcag ttcattcatt tccgtttgca ggacttttat cttttcggcc
      241 acctctttgc gcatcgaggt aacttgttgc cagtcaactt cttcacgttg ggcagcccat
      301 tctttgtagg ctgttttatc cttgggctta aaccgcaaat cagaaacaac cacatcgtga
      361 agagcactga tggcttcgcg aaaggcgatg tttttccgca attcagcgac aaaaaacgtc
      421 ggctcacgct tcaggtctgg cgcaaatgac agcgcagtgc ggtttgcctg attatcaaca
      481 cctgagtttc cccggtaagc atagtcaaac tgcatagcgc acctcggcgg gtttgactgc
      541 cattggcagc gggatggatg ggtatttctg atgaagtcgg accatgattt caatggttga
      601 agccttgtcg ccaatcgcca tggtcaccga ttgacggctc atgacctcgg ccaccagttg
      661 agcggcggct tcacttttga tggcttcagc ttctaaaaac tggagaatgc gagttttcgc
      721 caccctggct ttgttcacgt gtgaaagcac agtcacaaaa tatagggtga gttgctccag
      781 ccgttcgacg tttccagccg catatccttc cagataattg gtggcaaaca tttgcaacgt
      841 tgccgacgga tgctcgctga gcttgagcaa atattcctgg ccatcggctt cggtaaagta
      901 gcgggtgatc atctcacggc caaattgctg gatatcgggg cgaatactat cgcataaact
      961 gaccagaatc tgcgggttaa aatcactttc tgaaaactcc tcacgcaaga ttttcacccc
     1021 aaactgccgc aaatcaggcc atttagaatc aagcagccgg atcgccttgc ccatttcttc
     1081 atgatttcca cgcaatcgcg gcacaatggc ctgaaacagc gcctgaactg cttcacgaac
     1141 tgagagcatt tcatggttgc acagccgtgt aatgtcgtcg gttgtcagct cctcagccca
     1201 ggcaacattg agcttgagca aatagccgcc aacatcctgg gcagcggaag cacgggattt
     1261 gatgagccgc cagaccagtt ctttcgagac ctggcgcccc cacatgggat tgaggtcttc
     1321 tttgagcacc agcaccagat gtgagtagac tccatcgttg ggttgtggtt tgagaagcga
     1381 ttcaagcagg agtgtcgaaa atccaagaat aaaatcagga tgcgtctctg ccgcaccaag
     1441 gcggcgaata agaggccgga tagcctgacg caaatcgggc agcgcatgaa tgcaaattgc
     1501 ccgcaacaca cctttgcggt tgacgagcaa ttcatctggc aactcgctca gcaaccggat
     1561 cccaatcgcc cgaatggttt caaaatttga ctgaatcagc gcatgaatca ggctttccgg
     1621 caggttatgg gctttggtct cgtggttgag cagaatattt ccgccgagtt cctgaacttc
     1681 aaccatcggg tgcgtcacca gatcagccac tacattgagt ccaatcgtcc gcaactgcgc
     1741 gctaaacacg gtaaagaccg tttcggcaat atctttggct tgttcagcct gatgaggttc
     1801 aagcaccagt aaatgggcaa tcagtttgac aatacaggct ctggcagcat cttcggtaaa
     1861 ggaaactgag cggagcagtg tccgggcaaa aacgcgagtg tcagcataca aactggaggc
     1921 cagtccagcc agaaaaccgc tgtccttcac aaaccggtca cggctggtgg tgatccactg
     1981 gtgggcctgc tgacgggcag cgccatacgc acaactggcc acagccagaa ccagttcaaa
     2041 atcaggtact tccggcgtat atcggacgtt tgccagttca aacccaaact gggctgttat
     2101 ttcataaggc cgatttaata acatcacaac agcatcaact ggaagctgcc cgcaaaactc
     2161 agtacaggct cgaagtgccc gcacggcaaa ctgatgcact ggcagacagt tgctttctga
     2221 cagcaaatgg agcaggccaa ctggcatggt ttcccagagt ttggggaaag cttcttcgcg
     2281 cacagtgggg acaggctgac caggacgagc attgggtttg cagcgccaga ccatacttcc
     2341 ctttggccga atatagcggg ggctgtggtg atacaaaatc gcgttgaagg caaagtaggg
     2401 agcaaacgta tcccaatgat tggtcaccgc aacatagtcc actgagtgcc gtccacgttg
     2461 attggtggtt ggtcgcgcga catatcgggt tgacgttcgt gtgggatgag catcagcatc
     2521 tgaatagggc agcaggacac caaccgccat tttcacaaaa tcatggtcac cgagttcacc
     2581 caaccggcga agcgtgcgcc agactcgaat gcgcagaaaa ttgcgggtgt tctggctgta
     2641 agccagtttt gacgaggctg atttcagtgt tttcttgacg tcttccgtcg aatgggtctg
     2701 gctcgaatcg tcaatgtagt aactgctgta actccccagt tttcgaaaca tcggtttttc
     2761 tttttcaaac cgataggcaa ggatgccaaa gacttcggca tcccgccgat attcagccgc
     2821 tttgaaaatg tgccgaaagg cccgaaagta atttggtcgg agtggcgcgc ttttcaacaa
     2881 tccaagcaat gccgggcgaa cggtctgact gtcaatcaga taaatcgtat cgagcacgct
     2941 gtaatgtgac ggccctccgc tctcaagata gccacgcaag gcagaagtaa attcttcagg
     3001 tttcccagta cgagcagttt ctttgagcgg tgacggcagc cgatctacca tatccgattg
     3061 aaattcagcg cgggtttccg gatcagagag tttgatcaga gcttcgcagg caatccggcg
     3121 gacatgttca ggcgtggcgg catcgccata aattcgccca agcgtcgaaa cggttttttc
     3181 atcaccacac cagccaagcg cccaggcgat gcaataatcc cggagcgcat cacccgtgcc
     3241 aatcaatcga atcagaagcg gtgccgcgtc gcggagtttg agttcccccg cccgccagat
     3301 gacacgttcc agcggccagc ctttctttgc tggctggcgt tttccggtca agactgaacg
     3361 aacggccccc agcactccgg tcgtgacggg cttgcgctga gccagttcag gatgcgccag
     3421 taaatggagg atttgttgat ttcgagcttc ctgattgggt ggaccgagag gtgtggtggg
     3481 tttggcggca ggttccgtgg cagcaggttc gggagacgca ccttcccgat accctttttt
     3541 ggtttttgat ttgaccaggt cagcaaatat tcgttcgcca tcagcgagtg agaccggcga
     3601 aaccgtctgg gtgccttctt tgagggtcgt tccacgccgt ccgtaccgat agttcaccac
     3661 ataccgattc tggctaacct cacacaagtc aacttcatag accttgtcag atgagccttc
     3721 gcgaaacgaa agaagtgttt gcttgatgag cttcatggga acctggcttt ttgttcttgg
     3781 ttcttggttc ttggtgcttg gttcttagtt cttagttctt ag
//
LOCUS       DAOQDO010000299         3821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17123_length_3821_cov_3.430165, whole genome shotgun sequence.
ACCESSION   DAOQDO010000299 DAOQDO010000000
VERSION     DAOQDO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17123_length_3821_cov_3.430165"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            738..3329
                     /locus_tag="PKZ53_06125"
     CDS             738..3329
                     /locus_tag="PKZ53_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ40048.1"
                     /translation="MSREEKRKEIRDHHTILQAIKEIGAISELVEYESGHGYKYGVDI
                     EVAIYGRNYGPGKKVGPYIRMFEYPPGAEIVHQGDWETNTFFIIEQGAAEVFVDIEGG
                     QQKQVAVFEAGRPVGEMGVIAGVARAATVKAHHERGIRVLEVQRPALRILRKLRKFGT
                     ALDLTYRDNGRKSTLTTINLSEDLRKQLNDISEFKVIVRGHTICSEGTFIDKLYIIKD
                     GWIERSSSSKQGPVTDYMGPGYALGMNALTIDRARWTFKATALGRTELLEISLSKLKA
                     NPALLKLLQTEFEPYGTIGTPLQPVKELTFTPAAKTAQKRILDKGLADANNLLLMDMD
                     LCVRCGNCSLACHEIHGQSRLTRRGIHVLRPKHADQPKLDQSLLAPAVCLHCKDPECL
                     TGCPTGAIARFSGGQVDIIPSLCIGCGDCATQCPYNSISLIPRDDLKRSPATGAKAAP
                     AKTAGPAKGGDRKGQPAPEPAKEPFAKQLFTSLKLKYDEKPKPVIAEDNLVAVKCNLC
                     NDTPLNPKVDGKPVYKDHKYNCEENCPTGALMRVNPSQYFEEIGRIKGKAFRQDGSTL
                     YGSQFGWKDTGKQIAHTLGIVATLALCGGTMAGIVSYGLGTPLFNTQWFNLRWITGLV
                     GLLGIVVVMLYPGRRQVWRKRAGALRYWMLAHAYAGVIAGVVLLLHGGTHLGGILTSV
                     LMISFDLVILTGLIGLLLYWIGPRTLTQIEGEPLLVEDLIRRRQELYQEIADVTLTSQ
                     SEAEQQGRQQQFATDFLGTRDKVLDLTTSLGFLMRQYFKRESLDDLLKSIHTLFESDT
                     SKLDKREKDAFLRLLDAAVIIRRIDALIYIHRALKIWLPPHVISTSVMLATMLIHIVQ
                     VTYYLWK"
     gene            3384..>3821
                     /locus_tag="PKZ53_06130"
     CDS             3384..>3821
                     /locus_tag="PKZ53_06130"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ40049.1"
                     /translation="MTATSSRFVIKRKDLYEDDLVLELDGLAIGSALGSDLLLNHPTV
                     SRIHAGIREIDDSFWLTNLSKSNGTLVNGALIETVQIQPGDTIQIGIFLIRPAFDVPD
                     QITLEIDRQIDSVSAGNRAAGILGNPIQAAAEGKTIKLDPSLFA"
BASE COUNT          914 a         1036 c          943 g          928 t
ORIGIN      
        1 actgttttct cacttgaagt caactagggt tcttttgtat cgacggtact aattctatat
       61 acacgcgaaa tgaatcgatt tgtttcttag ttataaaagc cgggtttcgc ccgtgtagcc
      121 gatgtcctac attgatgtta atttgtcaca agccttccgg tttgagatgg cgcctgcctg
      181 ttttcgctct gttctgcagg caactgacct cgaaatggac tttccaaaca aaagtttctg
      241 gttcaaaaat cagccaagcc ggagtatact gcgccgcact ccccttgagt ggttccatca
      301 gttttccacc tgtacccaat tctttcgaaa gacagcactc ccagggacct cactttttct
      361 gtcgggggcg gttcagttca tttgttttga agcttcgttt tttcaacgac acagcctgaa
      421 cctggggata ccttctttgg gcaattcttg tcgctcacat tcaaatcgag ccagttttca
      481 aatacctgtt ttttgatttt tcatttttcc ctgcctcgac tggatcgaga aaaaaatctt
      541 ccagtttgct ttttcactga cacctgcaac accgttgatt caaaccgctc gtgctcgtgc
      601 ccgcgccgct ggtgtggttc tatttctccg acagtgtcag tgcccacaac acgttctttg
      661 caactaatta caagctgaac cacctgggtc agtcgaagaa agccatcctc tcactttcat
      721 catttcacag gggttatatg agccgagaag agaagcgtaa ggaaattcgc gaccatcaca
      781 ccatcctgca agccatcaag gaaattggtg cgatcagtga actggtcgag tatgaatccg
      841 gtcatggcta caagtatggc gtggacatcg aagtcgccat ctatggccgg aattatggtc
      901 ctggtaagaa agtcggtccc tacatccgca tgtttgaata tccaccgggg gctgaaatcg
      961 ttcaccaggg cgactgggaa accaatacct tttttattat tgagcagggc gccgccgaag
     1021 tcttcgttga tatcgaaggt ggccagcaaa aacaggtagc ggtgtttgaa gccggacgcc
     1081 cggttggcga aatgggcgtg attgccgggg tcgcccgtgc ggccacggtc aaagcccacc
     1141 acgaacgcgg catccgggtg ctcgaagtcc agcgaccagc cttgcgcatc ctgcgcaagc
     1201 tcagaaagtt tggaaccgcc ctggacctga cctaccgcga caatggccgc aaatcaaccc
     1261 tgaccaccat caacctttcg gaagacctga gaaagcaact caatgatatt tcagagttta
     1321 aggtcatcgt tcgtggtcac acgatttgta gcgaaggcac cttcattgat aagctctata
     1381 tcatcaaaga cggctggatc gagcgctcat ccagcagcaa gcagggaccg gtcaccgatt
     1441 atatgggccc cgggtatgct ctcgggatga acgccctcac cattgatcga gcccgctgga
     1501 cgtttaaagc cacggcgctt ggtcgaaccg aactccttga aatttccctc agcaaactaa
     1561 aagccaatcc ggcgctgctc aagctcctgc aaaccgaatt tgaaccctat ggcacgattg
     1621 gcaccccgct gcaaccagtc aaggaattga cctttacccc ggcagcaaaa actgcacaaa
     1681 aacgcattct ggacaaaggg ttagccgatg ccaataactt gctcttgatg gatatggatt
     1741 tgtgcgtccg atgcgggaat tgttcgctgg cctgccacga aatccatggc cagtcccgat
     1801 tgacgcgccg gggcatccac gtcttgcgcc ccaaacatgc tgatcaacca aaactcgatc
     1861 agagcctgct ggcaccggcg gtctgcctgc actgcaaaga cccggaatgt ttaaccggtt
     1921 gcccgaccgg ggcgattgcc cgattttctg gcgggcaggt ggatattatc ccttcgctct
     1981 gtattgggtg tggagactgt gccacccagt gcccctacaa ttcaatttcg ctcattccac
     2041 gcgacgacct gaaacgctca ccagccacgg gtgccaaggc agctccggct aaaacggcag
     2101 gcccggccaa aggtggcgac cgcaaaggcc agccagcccc cgaaccggcc aaagaaccgt
     2161 ttgccaaaca actcttcacc tcactcaaac tcaaatatga cgaaaaaccc aaaccagtga
     2221 ttgctgaaga caatctggta gccgttaagt gcaatctgtg caatgacacg ccacttaatc
     2281 ccaaagtgga tggcaagccg gtctataaag atcacaagta caattgtgaa gaaaattgcc
     2341 caaccggcgc gctgatgcgg gtcaacccca gtcagtattt cgaggaaatc ggacgcatca
     2401 aaggcaaagc ctttcgtcag gatggctcga cactgtatgg aagccagttt gggtggaaag
     2461 acactggaaa acaaattgcc cataccctgg gaattgtggc gacgctggcg ctgtgcggtg
     2521 gaacgatggc tggaatcgtg tcctatggac ttgggacacc cctcttcaat acccagtggt
     2581 ttaatctccg ctggattacg ggtctggtcg gattgcttgg gattgtggtg gtaatgctct
     2641 atccaggtcg ccgccaggtc tggcgcaaac gcgctggcgc cttgcgctac tggatgctcg
     2701 cccacgccta tgccggtgtg attgccggtg tggttttgct gctccacgga ggaactcacc
     2761 tgggcgggat actcaccagc gtcctgatga tttcgtttga cctggtcatt ttaaccgggt
     2821 taatcggatt gcttctctac tggatcgggc cccgcacttt gacccagatt gaaggcgaac
     2881 cgttgctggt tgaagatctg atccgtcgcc gtcaggaact gtaccaggaa attgccgacg
     2941 tcaccttgac ctcccaaagt gaagccgaac aacagggccg tcagcagcag tttgcgaccg
     3001 attttctcgg aacccgcgac aaggtgcttg atctgaccac gtcactcgga tttttgatgc
     3061 ggcaatactt caagcgcgaa tcacttgatg atctgctcaa atcaattcac accttgtttg
     3121 agtccgacac ctcaaaactc gacaaacgcg aaaaagatgc cttcttgcgg ttgctggatg
     3181 cagccgtgat tatccggcgg attgatgcgc tcatctacat ccatcgtgcc ctcaaaatct
     3241 ggttgccacc acacgtcatt tcgacttcgg tcatgctggc aaccatgctg attcatattg
     3301 ttcaggtgac ttattacttg tggaagtaag acatttgggt tccggattcc ggattccggg
     3361 tttttgcttg aaagggaacg tctatgaccg ctacgtcgtc gcgatttgta attaagcgca
     3421 aagatctcta tgaagatgac ctcgtgctcg aactggacgg gttagccatc ggctcagccc
     3481 ttggaagcga tctgttgctc aatcacccaa ccgtatcacg catccatgcg ggaattcggg
     3541 aaattgacga cagcttttgg ttgaccaacc tgtccaaatc caacggaacg ctcgtcaatg
     3601 gagcattgat tgaaaccgtc caaatccaac ccggtgatac catccaaatt ggaattttcc
     3661 tgattcgccc ggcatttgat gtgccggatc aaatcaccct tgaaattgat cgccagattg
     3721 actcagtttc cgctgggaat cgggcggctg gtattctggg aaatccaatt caggcagccg
     3781 ccgagggcaa aacgatcaag ctggatccaa gtttgtttgc c
//
LOCUS       DAOQDO010000300         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17150_length_3817_cov_3.773259, whole genome shotgun sequence.
ACCESSION   DAOQDO010000300 DAOQDO010000000
VERSION     DAOQDO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17150_length_3817_cov_3.773259"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PKZ53_06135"
     CDS             <1..1221
                     /locus_tag="PKZ53_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNJ40050.1"
                     /translation="IVKIESLDESMDVYDIEVPNTHNFALASGVFVHNSAKQGRDRRT
                     QAIMPLKGKILNVEKSRYDKMLSHSEIGALIMALGTGIGKSDFDINKLRYHRIIILCD
                     ADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLFKVKKGRSEQYVLDERELNRYL
                     MRKATEDVKVKVEATGKEYEGRELAKFIEKLIEFENYYNKLLRRLLRDTKLTDVVLQV
                     FGGTNGIADGVKLHKVFEDESRLGRLEAALIDAGYSTELTRDEEHNLWSVNVTGKNGV
                     VRIDWELSTHVEFQKSLTMYQDLRNAMQSSFTVANGGQPAQIPSYKQLAEHVLSVAKK
                     DINIQRYKGLGEMNPEQLWETTLNQGKRTLLQVRVNDAVETDEIFTILMGDTVEPRRK
                     FIEDNALDVKNLDI"
     gene            1479..2441
                     /locus_tag="PKZ53_06140"
     CDS             1479..2441
                     /locus_tag="PKZ53_06140"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNJ40051.1"
                     /translation="MTEITYFVPEEEAYARLDEFIQRHDFSFPISAIRKAIEDGNVRI
                     NSKVQTAGWRLRQGDKVTVKLEGYRGQALEAENVPLEVLYEDAFLIAINKPPDMLSHP
                     SVKERSGTLLNALLGYYQQKKDVEALKMWPALAHRLDRDTSGVIVAAKQERAVEKLFV
                     AFRDRKMKKVYQAIVFGIPEPEQGVIEAPIGRHPVLWPRWRVMDDGKSAKTAYRVTGA
                     RNGFSLLELEPLTGRTHQLRIHLAHIGHPIVGDHTYGRALNKAFAQSHPELKIKHHFL
                     HAAKLNFTHPMRREEIHLEAPMPQTMQEFWESGVVNKDVELQGN"
     gene            complement(2515..2589)
                     /locus_tag="PKZ53_06145"
     tRNA            complement(2515..2589)
                     /locus_tag="PKZ53_06145"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2555..2557),aa:Asn,seq:gtt)
     gene            complement(2799..>3817)
                     /locus_tag="PKZ53_06150"
     CDS             complement(2799..>3817)
                     /locus_tag="PKZ53_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40052.1"
                     /translation="WNSRKVIDPIESRFAMKLKVLYHNFCFDGACSAAVFSTFYRRKF
                     YPDVEISYHGLTHRPGSLFEPGLFDDADEFAIVDFKYNSDDRLTWWFDHHQSAFLSAE
                     DEAHFNQDTRGRKYFDPNYKSCTKYIADIAREKFDLDLPELTELIQWADLIDGAQYES
                     PENAINLKEPAPQLALVIESNRDQSLLHHIIRLLQVKSLAETAADERVQAVFAPLLAR
                     HNQAVEIIQSAGECSDGVVYFDVSDHDIEGYNKFIAYCLFPKARYSVGVSRGATRSKI
                     SIGYNPWSGMERTHNLAKICERYGGGGHAVVGAISLAPEDLEQAREIGKAVAHELRTT
                     NTLG"
BASE COUNT         1003 a          959 c          977 g          878 t
ORIGIN      
        1 atcgtgaaaa tcgagtcgtt ggacgagtca atggatgtgt atgatattga agttccaaat
       61 acacataact ttgcattggc aagcggggtt tttgttcaca atagtgcaaa acaaggtcgt
      121 gaccggcgaa cccaggccat catgccgctc aaggggaaga ttctgaacgt tgaaaaatca
      181 cgctacgaca agatgctctc gcatagcgaa atcggcgcgt tgattatggc gttgggaacg
      241 ggaatcggaa aatcggactt tgatatcaac aagctccgct atcaccgcat catcatcctt
      301 tgcgacgccg acgtggacgg aagccatatt cgaacgctgc tgctgacgtt tttctaccgg
      361 caaatgcctg aattgattga gcgtgggtat gtctacattg cccagccacc gctcttcaaa
      421 gtcaaaaaag gtcgttcgga gcaatatgtg ctggatgaac gggaattgaa ccgctacctg
      481 atgcgcaagg cgaccgaaga cgtcaaagtc aaagtcgaag caaccggaaa agaatatgaa
      541 ggccgcgagt tggccaaatt catcgaaaaa ttgattgaat ttgaaaacta ctacaacaaa
      601 ctgctccgcc gcctgttgcg tgataccaaa ctgactgatg tggtgctcca ggtctttggc
      661 ggcaccaacg gtattgccga cggcgtgaag ctccacaaag tttttgaaga tgaatcacgt
      721 cttggccgcc ttgaagctgc gctgattgac gccgggtact ccaccgagtt gactcgcgat
      781 gaagagcaca atctctggag tgtcaatgtc accggcaaaa atggcgtcgt ccgaattgac
      841 tgggagcttt caacccacgt tgaattccaa aaatcactga cgatgtacca ggatttgcga
      901 aacgccatgc aatcgtcctt taccgtagcc aatggcggtc aaccagcaca gattccttct
      961 tacaaacagt tagccgagca cgtgctgagc gttgccaaga aggacatcaa catccagcgt
     1021 tacaaaggac tgggcgaaat gaacccggaa caactctggg aaactacgct caatcagggg
     1081 aaacggacct tgctccaggt tcgagtcaac gatgcggttg aaaccgacga aattttcacc
     1141 attctgatgg gagacaccgt cgaaccacgg cggaaattca tcgaagacaa cgcgctggat
     1201 gtgaaaaacc tggatatcta attcgttggg gctgaaaaat cctcgggctg aagacattgg
     1261 gctgaagact cgcaagctcg gggctgaaaa atcctcgggc tgaagacatt gggctgaaga
     1321 ctcgcaagct cggggctgaa gacgttgggt tgaagacttt gggattttgc cttgaacccg
     1381 atgttcttca gccccaagcc cgttttcttc aacccgatgt cttcagccct cagcccgatg
     1441 tcttcagccc tcagccctgg ttttctcagc cctaaaaaat gactgaaatt acctattttg
     1501 tccctgaaga agaagcctat gcccggctgg acgagtttat ccagcggcat gatttttcat
     1561 ttccaatctc ggccattcga aaagcgattg aagacggcaa tgttcgcatc aacagtaaag
     1621 tccagacggc tggctggcgg ctgcgacagg gtgacaaggt cacggtcaag cttgaagggt
     1681 accgtggaca ggcactcgaa gctgaaaatg tcccgcttga agtgctgtat gaagatgcgt
     1741 ttctgattgc catcaacaag ccgccggata tgttgtcgca tccctcagtc aaagagcgca
     1801 gtgggacgct gctcaatgca ctgctgggct attatcagca aaagaaagat gttgaggcgc
     1861 tcaagatgtg gccggcgctg gcgcatcgcc tggaccggga tacgtcagga gtgattgtcg
     1921 cggcgaaaca ggaacgagca gtggaaaagc tctttgttgc ctttcgcgac cgaaagatga
     1981 agaaggtata tcaggcgatt gtgtttggca ttcccgagcc ggaacaggga gttatcgaag
     2041 cacccattgg ccgccatccg gtgctctggc cacgctggcg ggtgatggat gacggaaaat
     2101 cagcgaaaac cgcctatcgg gtcacgggag ccagaaatgg attctcactg cttgaattag
     2161 aaccgctcac tggacgcacg catcagcttc ggatccacct ggcgcatatc ggacacccga
     2221 ttgtcggaga tcacacctac ggacgggctt tgaacaaagc atttgcgcag tcacacccgg
     2281 aactcaaaat caaacaccac tttctgcacg ctgccaaact gaactttacc caccccatgc
     2341 gccgcgaaga gattcatctg gaagcgccaa tgcctcagac gatgcaggaa ttttgggaaa
     2401 gtggagtcgt gaacaaggat gttgagcttc agggaaattg acaaacggca tttgaagata
     2461 tcgcccacaa agaaaaacgg gagcttttca gctcccgttt ttctttgaat gtgttggctc
     2521 ctcaggtagg actcgaacct acaacccttc ggttaacagc cgaatgctct gccattgagc
     2581 tactgaggaa taagctcctc atgagggaac gcgcaatata ggcaatgacc tttggttttg
     2641 tcaacacttt ttttcagcaa ccagggaaaa agagaatgaa gaagtcagta ccacctgcgt
     2701 cagtgggtgg gaaaatgaag aatgatcctg agatttcact ctgtcttctt caacccgctt
     2761 tcttcaaccc gacttcttca gccctgagcc ctggtttttc agcccagggt gttcgtcgtt
     2821 cgcagttcgt gggcgacggc cttgccaatt tcacgggcct gctcaaggtc ttctggcgcc
     2881 agcgaaatgg caccgaccac cgcatgaccg ccaccaccgt agcgttcaca aatcttggcc
     2941 aggttatgtg tccgttccat tccgctccag ggattgtagc caatcgaaat tttggaccgc
     3001 gtggcacccc ggctcacgcc cacgctatag cgggctttgg gaaacagaca ataggcaatg
     3061 aacttattgt agccttcaat atcgtggtcg ctgacatcaa aatacaccac gccgtcagaa
     3121 cattcaccag cactctgaat gatttcaact gcctggttgt gccgggcaag caacggggca
     3181 aacaccgcct gaacacgctc gtcggctgcg gtttcagcca gagatttgac ctgtaacaac
     3241 cgaataatgt gatggagcag gctttggtcc cgatttgatt caatcaccag tgccagttgg
     3301 ggggcgggtt ctttgagatt gatcgcattt tccggagatt cgtactgcgc cccatcaatt
     3361 aaatcagccc actgaatcag ttcagtgagt tcgggcaggt ccaggtcaaa cttttcgcgg
     3421 gcgatgtcgg caatatattt ggtgcaggat ttgtaattcg gatcaaagta ttttcggcca
     3481 cgtgtatcct gattgaaatg agcttcgtct tccgcagaca ggaaggcgct ctggtggtgg
     3541 tcaaaccacc aggtcagccg gtcatcgctg ttatatttaa agtctacaat cgcaaactca
     3601 tcggcatcat caaacaaacc tggctcaaat aagcttccgg gccgatgggt caatccgtgg
     3661 tatgaaattt caacgtcagg atagaatttc cgccggtaaa aggttgaaaa gacagcggca
     3721 gaacatgccc catcaaagca aaagttgtgg tacagcactt ttaacttcat tgcaaacctg
     3781 ctttcaatcg ggtcaataac ttttcgtgaa ttccacc
//
LOCUS       DAOQDO010000301         3813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17176_length_3813_cov_3.123204, whole genome shotgun sequence.
ACCESSION   DAOQDO010000301 DAOQDO010000000
VERSION     DAOQDO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17176_length_3813_cov_3.123204"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..418
                     /locus_tag="PKZ53_06155"
     CDS             182..418
                     /locus_tag="PKZ53_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40053.1"
                     /translation="MIAFPVLGPYFTIILGIIIFLALGGAIVYGLIQQKEGFFHFGIV
                     VGSIAVPAICGILFVLGIIALILSICASGGKGWS"
     gene            556..1794
                     /locus_tag="PKZ53_06160"
     CDS             556..1794
                     /locus_tag="PKZ53_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ40054.1"
                     /translation="MSSKKRLVILGTGFAAVSLVKKINLRAYEVTIVSPRNHFLFTPL
                     LPSTTVGTIEFRSIIEPIRKVRRGVKFLQGACTRVDEKNRTIHCKSIDDQVEFTVEYD
                     QLVISVGAENNTFNIPGVREHALFLKELTDARNIRERIVACLERASVPGISKKERDKL
                     LHFVVVGGGPTGVEFAAELHDLLDDDLKKSYPELVNQVRITLFEAGKSVLNSFDVTLG
                     DYTKKHFNRQGIEVRLETPVAQVGTDFLKLKTGEVIPTGLIVWSTGYGPTSFIKSLPF
                     EKDRSGRIFTNEFLQIAAEPTIYAMGDCATITGIQLPQTAQLAMQQGKYLAEALNKVA
                     RDKQPKPFKFVNLGMLAYIGESQALAEIPMAGVRGSGMTTFLFWRSAYLTRLVSLKNK
                     VLVLFDWFKTFIFGRDLSKF"
     gene            1817..2179
                     /locus_tag="PKZ53_06165"
     CDS             1817..2179
                     /locus_tag="PKZ53_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40055.1"
                     /translation="MTDDELNRKMEFIVEQQAQFAVDIQLLKEAQVSTNASIAQLTKD
                     VLMLAEAQSQMREVQSQMQEQAEADRQEMRDGINQMISIAEEMRDLARALVTAQIGTS
                     QRVSKVEDRLDRLESDNP"
     gene            2199..2810
                     /locus_tag="PKZ53_06170"
     CDS             2199..2810
                     /locus_tag="PKZ53_06170"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNJ40056.1"
                     /translation="MAFRVRQFQILALALVHLILFGVTGCAHSVASDAPGPKNSAVLQ
                     AQLPASILNEIAGYSGWRKANPKPYFVPNQTAVLCAAPVPQPKSNPNPHEDKFITVYV
                     NEIGRQALTEAVEPKFPVGSIIIKEKHPTRESTQPELLTVMIKRQAGFNPEAGDWEFA
                     AIDGDAKKIQAQGKLENCQGCHIPQKAQDYVFRTYMARGVRRL"
     gene            3150..>3813
                     /locus_tag="PKZ53_06175"
     CDS             3150..>3813
                     /locus_tag="PKZ53_06175"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNJ40057.1"
                     /translation="MYYNKGKTTKQAHVNVPEGTFEEQHGREGFFGPVSHLYHNHPPT
                     SWTRIEGPCRPRAFDANRIEASGTNQVTPLLYNNDVSFSIATWTQSMDHFARNGDGDE
                     IWFVHKGAGILETDYGPLTFSRGDYLVLPRGVTYRFDLAQDCFFVLIETNGTPLEQPS
                     RGIIGQYSLYDASAVRVPDPTPVTATQAEYEVRIKREGEYTSVYYPFHPLDIAGWKGD
                     LFP"
BASE COUNT          957 a          943 c          953 g          960 t
ORIGIN      
        1 ccctgaaccc tgaaccctga accctgaacc ctgaaccctg aaccctgaac cctgtcctat
       61 gaatcacttc aatcccccca accaaccaaa agcaggttca tcaagtggca aagagttctt
      121 gctcggatgc gggctcttta cgctgttttt ctttttgtta ggcggtgccg gactgggcgc
      181 catgatcgct tttccagtgc ttggccccta tttcaccatc atcctgggta ttatcatttt
      241 cctcgcactg ggtggggcga ttgtatatgg tctgatacag caaaaagaag ggtttttcca
      301 ctttgggatt gtcgtgggaa gcatcgcagt tccagcgatt tgtggtattc ttttcgttct
      361 tggaatcata gctttgattc tgagtatatg tgcatcaggt ggaaagggat ggtcctgaaa
      421 agacagggtt cagagtttgg ggaatgaaag ataatcagaa aaaggccaaa gagccaaaaa
      481 gatgaaaagc attgcttcaa aaaccctgaa ccctaatctg cctggcaccg agaagtgaaa
      541 aggagtctcc acacagtgtc ttcaaaaaaa cgtctggtta ttttagggac tggctttgcc
      601 gccgtcagtc tggtcaaaaa aatcaatctt cgagcctatg aggtaacgat tgtcagtccg
      661 cgcaatcatt ttttgtttac tccgctgctt ccaagtacca cggttggaac aattgaattc
      721 cgcagcatta tcgaaccaat tcgcaaagtc cgccgtggcg tcaaattcct tcagggtgcc
      781 tgtacccgcg ttgatgaaaa aaaccgcacc attcattgca aaagcataga cgatcaggtt
      841 gagtttaccg tcgaatatga ccagcttgtc atttcagtcg gcgctgaaaa caacaccttc
      901 aatattcccg gtgtccgcga acatgcgttg tttttaaagg agttaaccga cgcccggaac
      961 atccgcgaac gcatcgtggc ctgtctcgaa cgggccagcg ttcctggcat cagcaaaaag
     1021 gaacgcgaca aactgctcca ttttgttgtg gttggtggtg gcccgaccgg ggtcgagttt
     1081 gccgctgaac tccacgacct cctggatgat gatctcaaga aaagctaccc cgaactggtt
     1141 aatcaggttc gaatcaccct ctttgaagcc ggaaaatcgg tgttgaactc atttgacgtc
     1201 acgcttggcg attacaccaa gaaacacttt aaccggcagg gaattgaagt ccggcttgaa
     1261 accccggtgg cccaggtcgg gacagatttt ctcaagctca aaactggcga agtgattcca
     1321 accgggctga ttgtctggtc tactggctac gggccaacat cgtttatcaa gtcacttcca
     1381 tttgaaaagg accgctctgg tcgaattttc accaatgaat tcttgcagat tgcggctgag
     1441 ccgacgattt atgccatggg cgattgtgcg acgattacgg ggatccagct tccgcaaacc
     1501 gctcagcttg cgatgcagca ggggaaatat ctggccgaag cactcaataa agtagcccgt
     1561 gacaaacagc cgaagccctt taaattcgtg aatctgggaa tgctggccta cattggggaa
     1621 agccaggccc tggccgagat tcccatggct ggggtacgtg gcagtggcat gacgacgttt
     1681 ctgttttggc gttcggccta tttgacccgg ttggtcagtt tgaaaaacaa ggtgctggtg
     1741 ttgtttgact ggttcaaaac ctttatcttt ggccgggatt tgagcaaatt ctgaggttga
     1801 gtaaggaggg atggacatga cagatgacga gctgaatcga aaaatggaat ttattgttga
     1861 acaacaggct cagtttgccg tggatattca attgctcaaa gaagcgcaag tgagtaccaa
     1921 tgcttcgata gcccagctta ccaaagatgt gctgatgctg gcggaggctc aatcccaaat
     1981 gcgagaagtt caatcccaaa tgcaggaaca ggctgaagca gatcgtcagg aaatgaggga
     2041 tggcatcaat cagatgattt cgattgccga ggaaatgcgg gatctggctc gtgcgctggt
     2101 gacagcccag attgggacgt cacaacgcgt ttcaaaagtg gaagaccgcc ttgatcggct
     2161 tgaatctgac aacccctgaa cattcaattg aggtgaatat ggcgtttcga gttcgacaat
     2221 ttcaaattct ggctctggcc ctggttcatt tgattctttt cggtgtgact ggttgcgctc
     2281 attcagtcgc gtctgacgct cccggcccaa aaaacagtgc ggtgttgcag gcccagctcc
     2341 ctgcttccat tctcaatgaa attgccggat acagtggatg gcgaaaggcc aatcccaagc
     2401 cctatttcgt gcctaaccaa acggcggttc tgtgtgctgc accggttcct caaccaaagt
     2461 caaatcccaa tccacacgaa gataaattca tcaccgtgta tgtcaacgaa atcggtcgtc
     2521 aggcattgac cgaggccgta gagccaaagt tcccagtcgg ttccatcatt atcaaggaaa
     2581 aacacccaac ccgcgaaagc acccagccgg aacttctgac cgtgatgatc aaacgtcagg
     2641 ctggttttaa cccggaggct ggtgactggg aatttgccgc aattgacggt gatgcgaaaa
     2701 agattcaggc ccagggcaag cttgaaaact gtcaagggtg ccacattcca caaaaagcac
     2761 aggattatgt ctttcgaacc tatatggcgc gtggagtgcg tcggttgtag cactaccggg
     2821 ttccgggttc cgggttccgg gttttcgaac tttcgtccct tttgtccttt ctgtcctttt
     2881 ggttttttac cctgaacccg agaaagaaag cttttgcttg caagggaatt caagattgag
     2941 acaaagtagg gggtgcccaa acgttgagcc aacctcccgg tgctcaacac ctcgttgtcg
     3001 agaaatcaaa acatcttacc cgttatgagc tgaatccatt gaattatgaa aagcctatct
     3061 gattgaatta taactattta cctgttttat aattcataat tcacaattca tcactcatcg
     3121 ctggttttac cccttggagc acttcttgta tgtactacaa caaaggaaag acaaccaaac
     3181 aggcgcacgt caatgttcct gaaggtacgt ttgaagagca gcacggacgt gaaggctttt
     3241 tcggcccggt ttcgcacctc tatcacaatc atcccccgac cagttggacc cggattgaag
     3301 gtccgtgccg gccacgggcg tttgatgcca atcggattga ggcatcgggt accaatcagg
     3361 taacgccgct gctgtacaac aacgatgtct cgttttcgat tgccacctgg acgcagtcca
     3421 tggatcactt tgcccgcaac ggcgatggcg atgaaatctg gtttgtgcat aaaggcgccg
     3481 ggattctgga aactgattac ggcccgctga ctttttcacg tggagactat ctggttctgc
     3541 cgcgcggagt gacctatcgg tttgacctgg cccaggattg ctttttcgtc ttgattgaaa
     3601 ccaatggcac acctttggag cagcccagtc ggggtatcat cggccagtat tcgctctatg
     3661 atgccagtgc agttcgcgtg ccggatccaa cacccgtgac ggccacccag gccgaatatg
     3721 aagtccgcat caaacgtgaa ggcgaataca cgtcagtgta ttatccattt cacccacttg
     3781 atatcgccgg atggaaaggt gacctgtttc cct
//
LOCUS       DAOQDO010000302         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17203_length_3809_cov_3.761854, whole genome shotgun sequence.
ACCESSION   DAOQDO010000302 DAOQDO010000000
VERSION     DAOQDO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17203_length_3809_cov_3.761854"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="PKZ53_06180"
     CDS             complement(<1..1872)
                     /locus_tag="PKZ53_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNJ40058.1"
                     /translation="MKPIELRVAGLHSYRQEQSIDFTILGGSNLFGVFGPTGAGKSTI
                     LDAITLALFGRVGRASGGTQAVINQQEPEATVSFTFALGAGELQHVYRVERRYRRSKG
                     AGATEFSVESRVARLLKLEGDDLSKAAVLAEKERQVNQKVQQLLGFTEDDFSRAVVLP
                     QGSFAEFLKLKNTERRECLQRLFGLDEYGDNLTMRARNELNRVYSQIQACSHELNGIG
                     DASDEKVAEAEARLVESEQTVQTTKAALSAAQQHLIEVRAVAGIAGKLREAHSRHMEL
                     ARHQPEFDRKRERLDAAQRFEGLREDIRQFQQAQQEAQIAEETVAQAQSNFDEISLEL
                     QTAMAEFERALTALDRQKPALDHRRVELTNARGLAEKANQLEVQLNQLQPIKAQLVEN
                     CEQETRSLLNWTSQADTIQQERRTLLQELEQTRVTLDQRTHLDQVKQALTALEQAERD
                     YEKAQHDCQRRETELTKTQTDVRAKQKAHLLATEDLTLARTEVAVAEAIPALSDQEFA
                     EQARQLGDLRSVISPLARLEQSLKEERREYRETSSQLETTRGQVLEAKAEVDRAEGTV
                     AAAQQELEQLEHTLRQSQLENQASYLAQTLTPGMACPVCGSVEHPNPATAVGAEQIAG
                     "
     gene            complement(1909..3129)
                     /locus_tag="PKZ53_06185"
     CDS             complement(1909..3129)
                     /locus_tag="PKZ53_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNJ40059.1"
                     /translation="MRVLHTSDWHLGKPLEGRDRLEEQGKVLDEMVELAEAENVHLVL
                     LAGDIFDTFNPPAAAEDLFYDAIDRLAAGGRRAVVIIAGNHDQPSRLTASQPLAGRQG
                     ISILGVPDDCPPTGGKTGGVQCIRSARSLVELAIPGCDHTAVIAALPYPSEARLNQVL
                     SESTREDLLQQAYAARVRSVLTELSAAYRSDAVNLAMSHLFVSGSQESGVESGERQIQ
                     VGGAYSVTAADFPETAQYVALGHLHRPQSVGGQERVRYSGSPLAYSFSETHTKSVVLL
                     DVEPGKPADIRQIQLSAGRPLVVWKAREGMAQALAWCDEGRDAEAWISLEVHSDRPLE
                     PLDISELRKRRERILPIKFVPTGMVQVDAPEKRSSLSQEELFRQFYRSRRKGDPSEAL
                     VQLFLELSQTTDSE"
BASE COUNT          825 a         1062 c          946 g          976 t
ORIGIN      
        1 cccggcaatc tgctcggcac caacggctgt ggccgggttt ggatgctcga ctgaaccgca
       61 caccgggcag gccatcccgg gtgtcagcgt ctgggccaga taactggcct ggttttcaag
      121 ctggctctgg cgcagggtgt gttcgagttg ctcaagttcc tgttgagcag cagcaacggt
      181 tccttccgcc cgatcaactt cagctttagc ttccagcacc tgaccacgag tggtttccag
      241 ttgtgaactg gtttcccggt attcgcggcg ttcttctttt aaagactgtt caagacgtgc
      301 cagcggtgaa atgactgacc gtaaatcgcc aagctgacgt gcctgttcag caaattcctg
      361 atcgctgagt gctggaatgg cttcggcaac ggccacttcc gtccgggcca gggtgaggtc
      421 ttcggttgcc agaaggtggg ccttttgttt ggcccggaca tcggtttgag ttttggtcag
      481 ttcggtttcg cgtcgctgac aatcgtgctg ggctttttca tagtcacgtt cggcctgctc
      541 caacgctgtc aatgcctgtt tcacctgatc gagatgcgtt cgctggtcga gggtaacccg
      601 tgtctgctca agttcttgca acagagttcg ccgttcttgc tggatggtgt cggcttgact
      661 cgtccagttt aaaagagacc gggtctcttg ctcacaattt tccacaagct gggctttgat
      721 tggctgaagc tgattgagtt gtacctcaag ctggtttgct ttttcggcca atccacgagc
      781 attggtcaat tccactcgcc ggtgatcaag ggctggtttt tgcctgtcca gggccgtcaa
      841 cgcccgttca aactcagcca tcgcggtttg aagttccaaa gagatttcgt caaaatttga
      901 ttgggcctgc gccacggtct cctcggcgat ttgggcttct tgctgggctt gctgaaactg
      961 gcgaatatcc tcgcgcagtc cttcaaagcg ttgcgcggca tccagtcgtt cccgtttacg
     1021 atcaaattcc ggctggtgac gagccagttc catatgcctg gaatgtgcct cccggagctt
     1081 cccagcaatt ccagccaccg ccctcacctc aatcaaatgc tgttgagcgg cactgagcgc
     1141 tgcttttgtg gtttgaacgg tttgttcgct ttccaccagc cgggcctcag cttcagctac
     1201 tttttcatct gaagcatctc cgattccgtt gagttcatga ctacaggcct gaatttggct
     1261 atatacccgg tttagttcgt tccgggcgcg catagtcagg ttatctccat attcatcgag
     1321 cccaaacaat cgctgcaggc attcacggcg ttcggtgttt tttaatttca agaattccgc
     1381 gaagcttccc tgcggcagca caaccgcccg tgaaaaatca tcttcggtaa acccaagcaa
     1441 ttgctggact ttttgattga cctgccgctc tttttcagcc agcacggctg cttttgacag
     1501 gtcatcccct tccagtttca acaagcgggc aacccgactt tcaactgaaa attcggtggc
     1561 gccagcacct ttactgcgcc gatagcggcg ttcgacccga tatacatgtt ggagttcacc
     1621 ggcacccaga gcaaaggtaa acgaaacggt ggcttcgggt tcctgttgat tgatcaccgc
     1681 ctgagttcca cctgaggctc gtccgacccg gccaaataat gccagggtga tggcatccag
     1741 aatcgttgat ttcccggcgc cagtcggtcc aaagacacca aacaggtttg aaccaccaag
     1801 gattgtaaaa tcaatggatt gctcctggcg atagctgtgc aatccggcga cacgcaattc
     1861 aattggtttc ataggctttg gactgctgcg gcttgacgca gccttttatt attcagaatc
     1921 ggttgtttgt gaaagttcga gaaacaactg caccagcgcc tcactggggt caccctttct
     1981 ccgggagcgg taaaactggc ggaataattc ctcctggctc aggctggatc gtttttccgg
     2041 tgcgtcaacc tgtaccattc ctgtgggcac aaatttaatc ggcaaaatgc gttcgcggcg
     2101 tttgcgcaac tcactgatgt cgaggggttc gagcgggcga tcactatgca cctcaagact
     2161 gatccaggct tcagcgtcac ggccttcatc gcaccaggcc agtgcctgcg ccatcccttc
     2221 gcgggctttc cacaccacca gcggtcgccc ggctgacagt tgaatttgcc ggatgtcggc
     2281 tggcttgccc ggttcgacgt ccagaagaac caccgacttg gtatgcgttt ctgaaaaact
     2341 gtaggccagt ggcgagccag aatatcgaac ccgctcctgt cccccaaccg attgtggtcg
     2401 gtgtaaatgc ccaagcgcca catactgggc ggtttcagga aaatcagcag cggtgacaga
     2461 gtaggcacca ccaacctgga tttgccgctc cccgctttcg acaccacttt cctgacttcc
     2521 agagacaaac aaatgactca tcgccaaatt gaccgcatca gatcgataag cggctgaaag
     2581 ctctgtcaaa accgaacgga cccgcgcagc atatgcctgc tggagcaggt cttcacgagt
     2641 gctttcactt aacacctgat ttaaccgggc ttcagaagga tacgggaggg cggcaatcac
     2701 agccgtatgg tcacatcctg gaatggccag ttcgaccagt gatctggccg aacgaataca
     2761 ctgaacgcct ccagtttttc cacccgtcgg tggacagtcg tcaggaacac ccaaaattga
     2821 aatcccctgc cggccagcca gtggctgcga ggctgtcaac cgtgacggct ggtcgtgatt
     2881 tccggcaatg ataaccactg cccggcgccc gccagccgcc agccggtcaa tggcgtcata
     2941 aaataaatct tcggcggctg ccgggggatt aaacgtgtca aagatatctc cggccagtaa
     3001 caccagatgc acattttcgg cctcggccag ctcgaccatt tcatcgagca cttttccctg
     3061 ttcttccagg cggtcccggc cttcaagcgg tttccctaaa tgccagtcag aagtatgaag
     3121 cacacgcata attcagtttg gaatcagtag ttagcaaatc gctaagttcg tttggatgaa
     3181 ttgcttgact attttctggt gacccaggtt caccaggaaa tagtatcagc aacttacata
     3241 aaatcaaaaa ttgattttgg tgttggatat caaaggatac aaaccagtta agtctatatc
     3301 aagctaacag gcgattacaa agttttttcc ggtgactact tttcttagga caggttccat
     3361 ccaggcggca attgggttga aaaaggctgt tcggttacgg aaacaatatc tcccatagac
     3421 gggcaccaaa ctgtttccac tcatagctcg ccaatcaggt aaactcttat ttttcaatga
     3481 attaaaatca gttcaatcct ggcacgagcc ttgccactaa gcgcctggag tgtccgccac
     3541 tccttgtaca tccctttttt tccattcagc aatgtcgcca gattggcggc actccacaaa
     3601 agcaaccagg cacattttcg tctttcaggg aattgtctat gcagggtctg cttcgcgaca
     3661 ttcgctttag tttccggctt ttgttcaaac gtccaggatt taccctggtg attatcctca
     3721 cactggcatt gggaattggt gccaacacgg cactctttac cgtggtcaaa accgcactcc
     3781 ttgatgccct cccctatgcc catcctgaa
//
LOCUS       DAOQDO010000303         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17204_length_3809_cov_3.312467, whole genome shotgun sequence.
ACCESSION   DAOQDO010000303 DAOQDO010000000
VERSION     DAOQDO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17204_length_3809_cov_3.312467"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..2180)
                     /locus_tag="PKZ53_06190"
     CDS             complement(105..2180)
                     /locus_tag="PKZ53_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40060.1"
                     /translation="MRFDQIVFTLIVFLGLLAGLTVVDTPTQAHPTKKKFRKKSAATK
                     LLKDTRAKMDVAKGELVTDGKYACCIKPVCDMCAKISGTCLCAVNVLQGKGACGECLE
                     GWKSGKGSLKGVTAEKVILLPTPVQPVAAIEVKPEPKIEAKQSEPEVPPATPEPQAKP
                     ATEAATEAATEPAPSHQHLTEARSMLNQAKRTLVAEGRYVCCIKGGCDSCTYEGECGC
                     GEKLVEALNNKKNVTGTPTKKTKDEDGVCGECYDGWHAGRGAYAGIDPAEVKLAPMDS
                     MPSSFALGTMFRQGSGTSWLPENTAMYAKMKEAGDWMLMYHPYAFTTYSNHTGPRGDS
                     KFYSANWFMASAQREVKGLTDNGNGVLMIRGMFSFDPITVGRSGYPLLFQTGETNRGN
                     PLVDRQHPHDLIMELALAYSAPITKNLVLSLYAAPVGEPALGPSAFPHRLSAMDNPEA
                     PLSHHWQDSTHIASGVFTVGLGMEKWKLESSIFTGAEPDENRWNIDQPKFDSWSTRFS
                     VNPHKNLSMQFSYGHLREPESLEAGTNIHRYTASVLYNHPFSNGSYLASAFIWGRNEK
                     RNPRFDGYGTNAFLYEATYVRKEQQSIFWRYENVEKDELFVGEDSEHRPSRTPVHHDE
                     GNPEIFRINRFTIGGVQNLPIPGDLQIGVGGSISFHAIPSALNPVYGNNPISTNVFVR
                     FRFKRWGLF"
     gene            complement(2379..3608)
                     /locus_tag="PKZ53_06195"
     CDS             complement(2379..3608)
                     /locus_tag="PKZ53_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40061.1"
                     /translation="MKNRWVVLIGLVLSLIMAQTTFAQDLTMVAGPSKKFGNYTISLR
                     LPEGGLVAEEEQQIEFRVVDHTKEDPVLGPAAIVRANIESIVSMPSMPSMPKVEEIAH
                     PEGVPGDYGLHPTFAHGGDYTLLLRITPPGGEPFVAEFPLFVADFDPAVKRVARPKPY
                     RVELKTEPGKVKAGEPTTLKLKVFGNLETSDATGKLTGKRETVQIKQFDIAHEKYLHM
                     IVVRKDLGFYIHEHPVIQPDGSFIYKDFVFPTPGEYQLFADVAPKGAGSQVLLANVKV
                     EGKGKPEIKPITLDQTKQTEMTVDGVKVSRKDNNFVPARKTSLLTFLLNKPTGEKVTD
                     VEPYLGAMGHLIMINQDSQTFVHSHPDERDAANGKNGEITFLVRPPKPGLYRGFFEFQ
                     RNGKVSRAEFVFEVKAE"
BASE COUNT          901 a          948 c          963 g          997 t
ORIGIN      
        1 cttggttctt ggttcttggt tcttggttct tggttctttg aaagtattga tgccaaagca
       61 ctaaatacca agtactaagt actggatttc ttcattcttc attctcaaaa aagcccccac
      121 cgtttaaacc gaaaccgaac aaacacgttg gtggaaatcg gattattgcc atagactggg
      181 ttcaatgccg agggaatcgc atgaaagctg atcgagccgc cgacaccgat ttgcaaatcc
      241 cccggaatgg gcagattctg gacgccacca atggtaaacc gattgatccg aaagatttca
      301 ggattgcctt catcatgatg aaccggggtc cgactggggc ggtgctcact atcttcaccg
      361 acaaacaatt catctttttc gacattttca tagcgccaga aaatgctttg ctgttctttg
      421 cgaacatagg tcgcttcata taagaacgca ttggtgccgt atccgtcaaa tcgagggttg
      481 cgtttttcgt tgcgtcccca gataaaagcg gaggccagat aggaaccgtt tgaaaatgga
      541 tggttataca gcaccgacgc tgtgtagcga tggatattgg ttccagcttc gagggattcg
      601 ggctcacgca aatgaccata ggaaaactgc atcgaaaggt ttttgtgcgg attgactgaa
      661 aaccgggttg accaggaatc aaacttcggc tgatcaatat tccagcggtt ttcatcgggt
      721 tcggcaccgg taaaaatcga actttccagt ttccattttt ccattcccag gccaacggtg
      781 aaaacacccg aagcaatatg cgttgaatcc tgccagtggt ggctgagtgg cgcttcgggg
      841 ttatccattg ctgacagccg gtgtggaaag gctgatggac caagggcggg ttcacccact
      901 ggcgcggcat acagcgaaag caccaggttt ttggtaatcg gagcgctata ggccagcgcc
      961 agttccataa tcaaatcgtg ggggtgctgg cgatcaacca gtggattgcc gcgattggtt
     1021 tccccagtct ggaacaacag cggatacccc gaacgaccaa ccgtgatcgg atcaaacgag
     1081 aacattcctc gaatcatcaa cacgccgttt ccattgtcgg tcagcccttt gacttcgcgc
     1141 tgggccgagg ccataaacca gttcgcgcta tagaatttcg agtctccgcg tgggccggtg
     1201 tgattgctgt acgttgtaaa ggcatagggg tgatacatca acatccagtc gccagcttcc
     1261 ttcattttgg catacatcgc ggtattttcc ggaagccagg atgttccaga accttgacga
     1321 aacatagttc ccagggcaaa ggaggacggc atgctgtcca tcggcgccag tttgacttcg
     1381 gctggatcaa tcccggcgta agcaccacga ccagcgtgcc atccgtcata acattcacca
     1441 caaacaccat cttcatcttt ggtctttttg gttggggtgc cggtgacatt cttcttatta
     1501 ttgagggctt cgaccagttt ttcaccacag ccacattcgc cttcataggt acaggagtca
     1561 caaccacctt tgatacaaca cacatagcga ccttcggcca ccagcgtccg tttcgcctga
     1621 tttaacatcg aacgggcttc ggtcaggtgc tgatgcgaag gtgctggttc cgtggctgct
     1681 tcggtggccg cttcggtggc tggtttggcc tgaggttcgg gtgttgctgg tggcacttcg
     1741 ggttcagatt gttttgcttc aatctttggc tctggcttga cctcaatcgc cgccacgggt
     1801 tgaaccggag tcggaagcaa tatgaccttt tcggcagtaa caccttttaa acttcccttg
     1861 cccgatttcc aaccttcaag gcattcacca caggcgcctt tgccttgcag gacattgacg
     1921 gcacacaggc acgttccaga gattttggcg cacatatcac agactggttt gatacagcag
     1981 gcgtacttgc cgtcggtcac cagttcacct ttggccacgt ccattttggc ccgtgtgtct
     2041 ttgaggagtt tggtcgccgc cgattttttg cgaaatttct ttttggttgg atgagcctga
     2101 gtcggggtat caaccactgt cagtccagcc agcaatccaa gaaaaacaat gagtgtgaaa
     2161 acaatttggt caaatcgcat aggaaaatac ctttagagga cagggttcag ggttcagggt
     2221 tcaggggttt cgaacttatg tccttttcgt ccttttctga acaggccgta aacgacctgt
     2281 cttagttcaa ccaccgctac gcggcaaaaa ccctgtaatt cactagtctt cgaaaacccg
     2341 gaacccggaa gcgcggaacc cggaagcgcg gaacccgatt attctgcttt cacttcaaac
     2401 acaaattcgg ctcgtgacac tttgccgttg cgctgaaatt caaagaatcc ccgatacagc
     2461 cctggtttgg gtgggcgaac cagaaacgta atttccccat ttttgccatt ggcagcatcg
     2521 cgttcgtctg ggtgcgagtg gacaaaagtc tggctgtcct gattgatcat aatcaggtgg
     2581 cccatggcgc cgagataggg ttcgacatcg gtcacttttt caccggtcgg cttattgagc
     2641 agaaaggtaa gcagcgaggt ttttcgggct gggacaaaat tgttgtcttt gcgcgaaact
     2701 ttcacgccat caaccgtcat ttcggtttgt ttggtttggt cgagggtaat cggcttgatt
     2761 tcaggtttgc ccttgccttc aactttgaca ttggccagta acacctgact gccagcccct
     2821 ttcggcgcca catcggcaaa gagttgatat tcgccaggtg ttggaaaaac aaagtcctta
     2881 tagataaatg aaccgtcagg ctggatcact gggtgctcgt gaatataaaa cccaaggtct
     2941 ttgcgaacca cgatcatgtg caggtacttt tcgtgagcaa tatcaaactg cttaatttga
     3001 accgtttccc gctttccagt cagtttcccc gtggcatcag aggtttccag gtttccaaac
     3061 accttgagtt tgagtgtggt tggttcaccg gctttgactt ttccaggctc ggttttgagt
     3121 tcgacacgat aaggtttggg acgggcaacg cgcttcaccg caggatcaaa gtcggcgaca
     3181 aagagtggaa actcagccac aaagggttcg ccgcccggag gtgtaatccg aagtaacagc
     3241 gtatagtcgc ccccgtgggc aaaggtcggg tggaggccat aatcacctgg aacgccttca
     3301 gggtgggcga tttcctcaac tttgggcatt gacggcattg agggcatcga aacaattgat
     3361 tcgatgttgg ctcgcacaat ggcggcgggc cccaaaaccg ggtcttcttt ggtgtggtca
     3421 accacgcgaa attcgatttg ttgctcttct tcagccacca gtccgccttc aggaagacgg
     3481 agtgaaatcg tgtaatttcc aaatttcttt gatggtccag cgaccatcgt taaatcctgg
     3541 gcaaaagtgg tttgggccat tatcaggctt aacaccaacc caatcagaac tacccatcgg
     3601 tttttcataa cagtcatctc cattgattct ttgaaaagat tgctgcctct ggttgggcaa
     3661 gtgacgtgcc agcgggtaaa atttggttaa ccgtatgaaa atacaggagt actgaaaatc
     3721 acagaatcaa ctttcaggtc aaaatttgtg gcatatgcag catcaggtgt ggcatatgcc
     3781 acactcgcat tcagtgccat tcataaccg
//
LOCUS       DAOQDO010000304         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17208_length_3809_cov_2.694193, whole genome shotgun sequence.
ACCESSION   DAOQDO010000304 DAOQDO010000000
VERSION     DAOQDO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17208_length_3809_cov_2.694193"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKZ53_06200"
     CDS             complement(<1..201)
                     /locus_tag="PKZ53_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40062.1"
                     /translation="MPDWIVRCICSVFIGLLILEVSTIRTLGQVQTPTSPFDFQPPFE
                     REMKAGEKHTYPVRLKANDFLKL"
     gene            complement(317..1573)
                     /locus_tag="PKZ53_06205"
     CDS             complement(317..1573)
                     /locus_tag="PKZ53_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ40063.1"
                     /translation="MDAKNTTQAADLVIVGAGAAGLMAAIWAGRTNPHRSIILLDGAK
                     KPGLKILISGGGRCNVTAEYADESAFAGSSRNAIKKVLRSFDVAQTIDFFRKLGVELK
                     REETGKLFPVTDSAQTVWQALLDAANAVNVRIKFPRRVSDIQKTESGFCVSGDWGTIE
                     TPTVIIATGGKSLPKTGSDGFGYQLVTALGHGLTERIFPALVPLTLPKDHFICGLSGL
                     TVPATLEVHSPTGKKLASFTDSTLCTHFGLSGPSVLDISRYFLDARMDNPDVRLVINW
                     LPEHTLEQLDRELQNPGKGTVLGFLRHHLPERLAKALCEFSAVDPALAGHQLTRDQRR
                     MLARNLTQLSLSNVGHRGFDFAEVTAGGVPLSELHLHTMESRLCKGLYLCGEICDVDG
                     RIGGFNFQWAWASGYLAGTSAGKTSG"
     gene            1835..2515
                     /locus_tag="PKZ53_06210"
     CDS             1835..2515
                     /locus_tag="PKZ53_06210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ40064.1"
                     /translation="MVSDNRLKAREKAQSLAKESFTKNDATGWFEQVYTAAAGNPDHI
                     PWADLKVNPSFATWAEGENLSGEGKRALVIGCGLGDDAEAIAALGFETTAFDISETAI
                     EWCQRRYPDSPVQYLVADALALPDEWKHQFDFVFEAYTLQALPVEVRPQVVAHLAQTL
                     APNGKLLLIARGRDPEDDPGSLPWPLVKSELDDFIRAGLTEVSFEDFWDQEDPPKRRF
                     RVLFSRLD"
     gene            complement(2520..2846)
                     /locus_tag="PKZ53_06215"
     CDS             complement(2520..2846)
                     /locus_tag="PKZ53_06215"
                     /inference="COORDINATES: protein motif:HMM:NF026933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm51 family immunity protein"
                     /protein_id="HNJ40065.1"
                     /translation="MAIYQIIEHDADKFSLIFSEFDQSEAIFNEYDMEGGGYAWHAVL
                     DALIRLHAPELIDTIDFDPEGSMFAAVSRSQKALEQLGSLLEHALEDEDFLREGIENA
                     DLELLD"
     gene            complement(2862..3377)
                     /locus_tag="PKZ53_06220"
     CDS             complement(2862..3377)
                     /locus_tag="PKZ53_06220"
                     /inference="COORDINATES: protein motif:HMM:NF040024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6210 family protein"
                     /protein_id="HNJ40066.1"
                     /translation="MNPVIILYDSIGLGLIISRPSGVFYSNQVGGHACMQPAIEGVYV
                     PLHDEVKNQEQQLHDYFTGPKWNGWGCYPLDETDADFIDGVLSQSMATSFLKVDRTRL
                     DESVEAWVYVKVSNQPPERPTTFDGKIATTASGATWRVTDFQLLPIPPEHFPMFGFGE
                     CTGILTWCNSD"
     gene            complement(3514..>3809)
                     /locus_tag="PKZ53_06225"
     CDS             complement(3514..>3809)
                     /locus_tag="PKZ53_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40067.1"
                     /translation="GLAFFLIILIEGFGYGGDGAYIIILVGPVVTFISMFLAMWLGEQ
                     GAMKAQSKAKANNQLLTRADSIRTIMYWTMLVNLGVWLLLLSTCAGIGLIGAM"
BASE COUNT          981 a          971 c          983 g          874 t
ORIGIN      
        1 cagcttcaga aaatcattgg cctttaatcg gaccgggtag gtgtgttttt cgccggcttt
       61 catctcacgc tcaaatggcg gctgaaaatc aaatggcgaa gtgggcgtct ggacctggcc
      121 cagggtgcgg atggtcgaga cctccagaat cagtaaacca atgaatacac tacagataca
      181 gcgaacgatc cagtcgggca tagagaaaaa tctccgggtt gaaaggcggg gatcctaccg
      241 aaaatttcca ggcattttaa ccacggaaaa cacggaaaac acggaaaaag atcagaggct
      301 gaaataaact ggcgcgttag cccgaggttt tcccggctga ggtaccggca aggtagccgc
      361 tggcccaggc ccattggaaa ttaaagccgc cgatgcgacc gtctacgtcg cagatttctc
      421 cgcagagata aagccctttg cacaggcgag attccatcgt gtggaggtgg agttctgaca
      481 acgggacgcc gccggcggtg acttcggcaa agtcaaaacc gcgatgaccg acatttgaca
      541 gcgaaagctg ggtgagattg cgggccagca ttcggcgctg gtcacgagtc aactggtgtc
      601 cggcaagagc ggggtctacg gcggaaaatt cgcagagcgc tttggccaga cgttctggca
      661 gatggtgacg cagaaagccc aataccgtgc cttttcccgg gttttggagt tcccggtcaa
      721 gctgctccag ggtgtgttca ggcagccagt tgatgaccag gcggacgtcg ggattgtcca
      781 ttctggcgtc cagaaagtac cggctgatgt cgagcaccga cggaccagac aatccaaaat
      841 gcgtgcacag ggttgaatcg gtaaacgaag cgagtttttt tccagtcggg gagtgaactt
      901 cgagcgttgc cggaacggtc aatccgctta atccgcaaat aaaatgatct ttgggcagcg
      961 tgagtggcac cagcgccgga aagatccgtt cggtcaggcc atggccaagt gctgtgacga
     1021 gctggtatcc aaaaccatcg gaacctgttt tcggaagact tttcccacct gtggcaatga
     1081 tcacggtggg tgtttcaatg gttccccaat caccggaaac gcaaaaccca gactcagttt
     1141 tttgaatatc ggaaactctg cgcgggaatt tgatgcggac attgacggcg ttcgccgcgt
     1201 caagcagcgc ctgccagaca gtttgggcgc tatcagtaac gggaaacagc ttgccggttt
     1261 cttcgcgttt gagttcgaca cccagttttc ggaagaaatc tatcgtttgc gcaacgtcaa
     1321 agctgcggag caccttcttg atggcattgc gcgacgatcc ggcgaaggcc gattcatcgg
     1381 catattccgc cgtcacattg cagcgtccac cgccggaaat caggatcttc aaccctggtt
     1441 ttttggctcc gtccagcaaa atgatcgaac ggtgcgggtt ggttcgtccg gcccaaatgg
     1501 cggccatcaa tccggcagca ccggcgccga cgatgaccag gtcagcagcc tgggttgtgt
     1561 tttttgcgtc cattttttta gggttcaggg ttcagggttc agggttcagg gttcaggtac
     1621 aaaattatca gtttgaagtt atcacgaaat acacggagca caggaaaaaa actacttcaa
     1681 taaaattggt tgacagtcac catttaattt ataactctct tgtttctcct gaaccctgaa
     1741 cctgggcgga tgacaggctg acaaggtgac aggggttttt tcatccctca tcccttccga
     1801 aaaccctgaa ccctgaaccc tgaaccctaa aactatggtc tccgataacc gactcaaagc
     1861 ccgcgaaaaa gcacaatccc ttgccaaaga atcatttacc aaaaatgacg cgactggctg
     1921 gtttgaacag gtttacacgg cagccgccgg caacccagat cacattccct gggccgatct
     1981 gaaagtcaac ccgagctttg ccacctgggc cgaaggtgaa aacctctctg gcgaaggaaa
     2041 acgagccctt gtcattgggt gcggcctcgg agacgatgcc gaagccattg cggcacttgg
     2101 gtttgagaca acggcgtttg atatttccga aacggcgatt gagtggtgtc agcgtcggta
     2161 tccggattca ccagttcagt atctggtggc agatgccctg gcgctaccag atgaatggaa
     2221 gcaccagttt gacttcgttt ttgaagccta cacgctgcag gcgcttccgg ttgaagttcg
     2281 gccacaggtc gtggcacatc tcgcccaaac gctggcacca aatggcaaac tgcttctgat
     2341 tgcccgtgga cgtgaccccg aagacgaccc cggcagcctg ccctggccgc tggtgaaatc
     2401 agaacttgat gatttcatcc gcgctggcct gaccgaagtt tcatttgaag acttttggga
     2461 tcaggaagat ccacccaaac gccggtttcg cgttctgttc tcgcggctcg attaaacact
     2521 caatctaaaa gttctaaatc agcgttttcg atgccttcgc gcaggaaatc ttcatcttcg
     2581 agggcatgtt cgagcaggct gccaagctgt tccagggctt tctggcttcg gctcacagcg
     2641 gcaaacattg agccttccgg atcaaagtca atggtgtcaa tgagttcagg agcatggagc
     2701 cggatcaggg catccaggac ggcatgccag gcatagcctc cgccttccat gtcatattcg
     2761 ttaaagatgg cttcactctg gtcaaactcg gagaagatca gactgaattt gtctgcatcg
     2821 tgttcaatga tttggtaaat ggccatggta tcaggtgttc ctcagtcgct gttgcaccac
     2881 gtcagaatcc cagtgcattc accaaaccca aacatcggaa agtgttcggg tgggattggc
     2941 aaaagctgaa aatctgtgac tcgccacgtg gcaccgctgg ctgtcgttgc gatttttcca
     3001 tcaaaagtag taggtcgctc agggggttga tttgacacct tgacatatac ccaagcttcg
     3061 accgattcat caagccgagt tcggtcaact ttcaaaaaac ttgtggccat tgactgagaa
     3121 agaacgccgt caatgaaatc agcatcggtt tcatccagtg gataacatcc ccagccattc
     3181 cacttcgggc cggtgaaata atcgtggagt tgttgttcct gatttttgac ttcatcgtga
     3241 agcggcacat agacaccttc aattgctggt tgcatacagg catgcccacc aacctgattg
     3301 gagtaaaaca cgccactcgg tctggagata atcaggccaa gcccgatgct gtcgtagaga
     3361 atgatgactg gattcatatt cgacaaatga ttctgtcgcg gaaaggttga gagtcgccct
     3421 gcgagtggga ttatcaaact caagtgatct cgccgcaacg gaagataaat ggaaatttga
     3481 ttagaaaaac aaactaccac aaggtccagg ccattacatt gccccaatca atccaattcc
     3541 ggcacaagtt gacaacaata acaaccagac tccgagatta acaagcatag tccaatacat
     3601 aatagtccga atgctgtctg ctctggttag caattgattg tttgccttgg cttttgattg
     3661 agctttcatt gcaccctgtt caccaagcca catggctaaa aacatggaga taaaggtaac
     3721 aacaggacca accaaaatga taatgtaggc accgtcacca ccataaccaa aaccttcaat
     3781 cagaataatc aagaaaaatg ccaatccaa
//
LOCUS       DAOQDO010000305         3805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17239_length_3805_cov_3.661067, whole genome shotgun sequence.
ACCESSION   DAOQDO010000305 DAOQDO010000000
VERSION     DAOQDO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17239_length_3805_cov_3.661067"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..771
                     /gene="cyaB"
                     /locus_tag="PKZ53_06230"
     CDS             178..771
                     /gene="cyaB"
                     /locus_tag="PKZ53_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNJ40068.1"
                     /translation="MNLETEIKVRGQNPEATLARLTEQGFSITVVQPRHFEDNWLLDT
                     ATSEMRARRSALRIRLAEGKTIVTYKGLPPARLRQENLKVREELEVVVDDAEVMVKIF
                     ERLGFRSFFRYQKYRTSYEIESASLGISLIATFDETPLGFFLELEGQADAVRATIASL
                     GFAPDQLSTESYIALQSAHCTAQGLPLQDMVFALSEE"
     gene            complement(846..1544)
                     /locus_tag="PKZ53_06235"
     CDS             complement(846..1544)
                     /locus_tag="PKZ53_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ40069.1"
                     /translation="MDQPITLITGGTGGLGQAVTQAFLQRGDRVIVTWSSERGRDELR
                     QLTDSQSVDLTLLPANVTDEASVNALFSRIEQDFQRLDNTVHLVGGFAGGLSVAETSL
                     ETFDHMVAMNFKSAFLVLRAAAALMQSQRSGHIVTVASRSAIQVAPGIGVYAASKAAL
                     VALTQTLALELGPFGINANVVLPGTIDTPANRRAMPDADPAAWTKPERIAQTILTLCS
                     PTLSGVNGVAIPIS"
     gene            complement(1796..1984)
                     /locus_tag="PKZ53_06240"
     CDS             complement(1796..1984)
                     /locus_tag="PKZ53_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ40070.1"
                     /translation="MFGSLGTTELLLILAIVVILFGAKKIPDLASGLGSGIRNFKKAL
                     NEDPEKDAQGGAPKTEAK"
     gene            2172..2588
                     /locus_tag="PKZ53_06245"
     CDS             2172..2588
                     /locus_tag="PKZ53_06245"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNJ40071.1"
                     /translation="MPQKTVKITDQVYQLIVQLALGEYPRESCALLGGNQGLFSSWYP
                     LHNVAELPERQYAAPPEALFQAMRQMRERHETELGIFHSHPSSAAYPSQTDIELAFYP
                     DATYFILAVRPSPLLRAFHIVHECVEPIEIQILAAP"
     gene            2664..3497
                     /locus_tag="PKZ53_06250"
     CDS             2664..3497
                     /locus_tag="PKZ53_06250"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNJ40072.1"
                     /translation="MTTLYEKAQEAAQFLSLQVDRTPDALVILGSGLGIFADALTDAK
                     HVAYHAVPHFPVSTVEGHRGQFAIGYCGNALLLVMQGRFHYYEGYDLELVTLPIRVAK
                     LMGIQRMIVTNSAGGLNTDFQPGDLMLIEDHINGLGANPLRGHNDSRFGHRFPDMTNA
                     YDPLYRKIATEESEKMGMRLQSGVYIALSGPCFETPAEIRMFQRLGVDAAGMSTVPEV
                     IVARHMGMRVLGMSCISNLAAGTTGQPLNHEEVLETGLLVSKKFTELLQRTIPRICQE
                     G"
BASE COUNT         1014 a          931 c          918 g          942 t
ORIGIN      
        1 gtcagtcgtc agtcgataaa acccggatat tgtagtgtgt ggagttcttg aaaagaatta
       61 ttcataactg actcaaatag aaccaaatac acttgacacc aattccaaaa tcccaacact
      121 tgacgactga cgactgacga ctgactactg actactgacg actggctggg gaatactatg
      181 aaccttgaaa ccgaaatcaa agtccgcgga caaaacccgg aagccacgct cgcccggttg
      241 actgagcaag gattttcaat caccgtggtt caaccccgcc attttgaaga taactggctt
      301 ctggataccg caacgtctga aatgcgtgcc agacgttcag ctttgaggat ccggctggct
      361 gaaggcaaaa ccatcgtcac gtataaaggc ttgccaccag cccggttacg tcaggaaaat
      421 ttgaaagtgc gggaagaact tgaagttgtg gttgatgatg ccgaggtgat ggtcaagatt
      481 tttgaacggc ttggattccg atccttcttt cgttaccaga aatatcgaac gtcctacgaa
      541 attgagtcag catcactggg catttcactg attgctacct ttgatgaaac cccgcttggt
      601 ttttttctgg agcttgaagg acaggccgac gctgtccgcg ccaccattgc cagcctcggc
      661 tttgcaccag accaactctc gactgaaagc tatattgcgc tgcaaagcgc ccactgtacc
      721 gcccagggat tgccgctaca ggatatggtt tttgctctga gtgaagagtg aatgagtctt
      781 ttacttttaa acgatctgac cacttcgtga cttcactatt tcacgacctg gctatttcgc
      841 tattctcaac tgattggaat tgcaaccccg ttgacaccag aaagcgttgg actacagagc
      901 gtgaggatgg tttgggcaat tctttcgggc tttgtccagg cggcaggatc ggcatctggc
      961 atcgcgcgac gattggccgg ggtgtcaatt gttccgggca gaaccacatt cgcattgatt
     1021 ccaaatgggc caagttccag ggccagggtc tgggttaaag caaccagggc agctttcgaa
     1081 gcagcataga cgccaattcc aggagcaacc tggatagcgc tccgggaggc aaccgtcacg
     1141 atatgacctg aacgttgaga ctgcatcaag gcagcggcag cccgcaaaac cagaaaagct
     1201 gacttaaagt tcatcgccac catatgatca aacgtttcaa gcgaggtttc cgcaactgac
     1261 agtccaccag caaagcctcc gacgagatgg acagtattat ccaatcgctg gaaatcctgc
     1321 tcaattctgg aaaacagcgc gttgaccgac gcttcatcgg tcacattggc cgggagcaaa
     1381 gtcaggtcaa ctgattgact gtcagtgagt tgacgtaatt cgtcgcgacc gcgttcagaa
     1441 ctccaggtca caatgacacg gtctccgcgc tgcaaaaaag cctgagtaac tgcctgacca
     1501 agtccaccgg tgccaccggt gatcaaagtg atgggttgat ccataatttc ctcattttgg
     1561 gaaaaataag aaaggacttt acactgccac tggcaggtaa agtcctggta atgaaaaaaa
     1621 atcagcgaat caatcggggg aagatagcaa tgaactgaag caaccgagga aaacgatttc
     1681 agatatttat gcaagcgtca cattttacag aaaatgtgtt ttgctcacgg ctctgccagc
     1741 cacaaataca cgaatttgaa aggcagtgtg agcactgaac ttattgtcag aaatgctact
     1801 tggcttcggt tttgggagct ccaccctggg cgtctttttc aggatcttcg ttgagcgctt
     1861 tcttgaagtt gcgaattccg gaacccagcc cggaagccag atcagggatt ttctttgcgc
     1921 caaacaagat caccacaatt gccaaaatca gtagcaattc agtcgttcca agagaaccaa
     1981 acatataaaa ctccttttgt ttatcgggaa aaatgattct agtctgaggg atagggagtg
     2041 tcaattcaga ctgttgtggg gtgttcctcg aaaatcccac tggtttctaa aataatccgg
     2101 ttacagccag tttactggaa gaaaagattt tttgagtgtg ccaacgtgta cggcttgatt
     2161 ggagactgaa gatgcctcaa aagacggtaa aaattacaga tcaggtctat caactcatcg
     2221 ttcaacttgc cctgggtgaa tatccacgcg aaagttgtgc gctgctcgga ggaaatcagg
     2281 ggctcttttc aagctggtat ccactccaca atgtagctga acttccagag cgtcagtacg
     2341 ccgctccacc cgaagcctta tttcaagcca tgcgtcagat gcgggaacgc catgagacgg
     2401 aattgggaat ttttcactcg cacccgtcat cagcagccta tccttcgcaa acagatattg
     2461 aactcgcctt ttatcctgac gcaacttact ttattctagc agttcgccct tccccattac
     2521 tgcgcgcctt ccatatcgtg cacgaatgtg ttgaacccat cgaaatccaa atccttgccg
     2581 ctccttgatt tggtccatga ggttcactcc cacagggtcg agagttttac tccattagaa
     2641 aaataaacga aggaaaccca cctatgacca ctctctatga gaaggcccag gaggccgccc
     2701 agtttttatc gttgcaagtt gatcgcacac ctgatgcact tgttatttta ggctctggct
     2761 tagggatttt tgccgacgcc cttaccgatg ctaaacacgt tgcttatcac gcagttccac
     2821 attttccggt ttcaaccgtt gaaggtcacc gtggtcaatt tgcgattgga tattgtggca
     2881 atgccttgtt actggtgatg caaggccgct ttcactacta tgaaggctat gaccttgaac
     2941 tggtcacgct ccccattcga gtcgccaaac taatggggat tcagcggatg attgtgacca
     3001 actcggcagg tggcttaaat accgattttc aacccggcga tttgatgctc atcgaagacc
     3061 atatcaacgg cctcggtgcc aacccgttgc gcgggcacaa tgattcccgg tttggtcatc
     3121 gctttccgga tatgaccaac gcctatgatc cactgtatcg aaaaatcgcc actgaagaat
     3181 cagaaaaaat ggggatgcgg cttcaaagtg gagtctatat tgctctcagc gggccttgtt
     3241 ttgaaactcc ggcggaaatc cgcatgtttc aacgacttgg cgtagatgct gccgggatgt
     3301 caacggtgcc ggaagtcatt gttgcccgac atatgggcat gcgagtcctt gggatgtcgt
     3361 gcatttcaaa tctggcggcg ggaaccaccg ggcaaccgct caatcacgaa gaagtgcttg
     3421 aaactgggtt gctcgtcagc aaaaagttta ccgaactgct gcagcgaacc atcccccgga
     3481 tttgtcagga aggatagaat gaagaatgaa gaatgatctc aattttgccc aaaatcgcaa
     3541 cgccttgctt aaagatcagt catgatcagg tcagtccccc tcggaaacag gaagtgacca
     3601 ggccattgag ttgaatcaac tgttaccata atcgagcctt cgcggtgacg aaactgactt
     3661 ccacgctgtc ctttagttca ggctcggtca accgtatttg aaaccaaaac tgaggagtcg
     3721 attggtattt tgggcggaat ttggggaata aagaatgaag atagggttta cgcagccttt
     3781 gaagaaggga cgtaaacctt tgaaa
//
LOCUS       DAOQDO010000306         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17248_length_3803_cov_3.718783, whole genome shotgun sequence.
ACCESSION   DAOQDO010000306 DAOQDO010000000
VERSION     DAOQDO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17248_length_3803_cov_3.718783"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..2062
                     /locus_tag="PKZ53_06255"
     CDS             380..2062
                     /locus_tag="PKZ53_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNJ40073.1"
                     /translation="MSTVLLVGGTVPTLAYVQKEKTVASSTSATTPEVIAKIDQILSN
                     AYPANTPGAAVLVSKDGKVVFRKGYGLADVEKKVPIQPDMIFRLGSITKQFTAVGILK
                     LVDQGKIALDDEITKFLPDYPTQGKKITIEHLLTHTSGIKSYTGMASFGSIMRKDVTP
                     IELVDIFKNEPMDFEPGTQWRYNNSGYVLLGVIIEKVSGKSYAQFLQETIFKPLGMKH
                     TFYGTEEASIPNRALGHTGDGKEFSYSAPMSMTLPYAAGALESNVDDLAIWNHAMASG
                     KLLKPATWKRAFTPYTLSTGKPTTYGYGLEFEQLQGEEVIEHGGGINGFITMGYWLPK
                     QKVFVAILTNQDSAEVQPSFVARKVAATMIGKGFKEYTAVTLDAKKLDEFVGVYLIED
                     KTTRTITRQGNQLYAQRTGGQKMEIFPASDNEFFYKSSFTVLKFVRDEAGKIVKMIKT
                     DEKGEESAIKTDQPAPKEREVAKVDPAIYDAYVGEYELVPGFVLTVTRDGDKLLTQAT
                     GQQRLEIFPESETEFFLKVVDAQLTFVKDADGKVNQVILHQGGRNMPAKRIK"
     gene            2214..3743
                     /locus_tag="PKZ53_06260"
     CDS             2214..3743
                     /locus_tag="PKZ53_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ40074.1"
                     /translation="MSESILSSIPKYEVVVVGAGIAGLTTAALLQKEGIQTLLLEAHD
                     KVGGCAGYFFHKGYTFDAGATVIIGLDPEGLHSYVFQQLGVAVPESTLIECVTVHLPD
                     RTVNLWHDQGKWERERVERFARTPDEARRLAAFWKQVDTTADVLWQMTAKQPVLPVRG
                     IRDLISNLRLVSPDVLWILPAAISTFDDILRRYQLSDHAELRAFLDGLLLITTQETAE
                     RAPFYNAAAGIDLYRHGIRRARGGMKTLVRTLLEAYRSHGGTIKFRQKVTEIEPRSQG
                     GYVVKTREGLIVEAKKVVANIPVWNVPQLVKADLTASFSRALKKADIGWGAFTLYLGV
                     DESVIPPDSALNHQVLQRYGEPFKDANSCFLSLSEAGDLRSAPAGKRTLVVSTHTEVN
                     TWYGLSLEDYTRRKQALTDQTLAAIRKVFPDIDRGLEVVLPGTPVTFENYTSRERGMV
                     GGLRTQLTNSNLLAISSDIGLKDFWLVGDTTFPGQGTMACTLSGINAWRDICGKLKKV
                     G"
BASE COUNT          986 a          882 c          991 g          944 t
ORIGIN      
        1 ccactggtga tctcggctga acaaattgat tttctggtgg atgtattgct caaagtcatt
       61 ccggaaatca ctggggaata gggctgaaaa aacctgggct aggggctgag ggctgagggc
      121 ttggggttga agaaatggtt ttatttcatc cctcatccct tcgattgccc tgagcttgcg
      181 agtcttcatc ccgaagtctt aaccctcagc cctcagccct cagccctggg ttcttcagcc
      241 ccatgatttc cggcgaacct caccagtttt cactccgtat agcgcccatc atcacaaatt
      301 ccaattcatc acaatcagtt cacagcacaa aggagtttca aacatgagga atccaggtca
      361 cctcttgggt gcaggtttgt tgtctacagt tcttctggtt ggaggcactg tcccaacatt
      421 ggcttatgtt caaaaagaaa aaacagttgc cagtagcaca tctgctacga caccagaagt
      481 tattgcaaaa attgatcaaa tactatcaaa cgcttatcca gcaaatacac cgggagcggc
      541 tgtccttgtt tccaaagacg gtaaagtggt cttccgtaaa gggtatggcc tggcggatgt
      601 tgaaaagaag gttccaattc aaccagatat gatttttcga cttggctcga ttaccaagca
      661 atttacagcg gttgggatcc tgaagctggt tgatcaggga aaaatcgcgc ttgatgacga
      721 aatcaccaaa tttcttccag attatccaac tcaagggaaa aaaatcacaa ttgagcattt
      781 attaacccat acttcaggaa tcaaaagtta taccgggatg gcatctttcg gttcgattat
      841 gcgaaaagac gtcacgccga ttgagctggt tgatattttc aaaaatgagc cgatggattt
      901 tgagcctggg acgcaatggc gatacaacaa ttcaggctat gttctgcttg gcgtgatcat
      961 cgagaaagtc tctggtaaat cctatgccca gttcttgcag gagactattt ttaagccatt
     1021 ggggatgaaa cacacttttt acggtacaga agaagcctcg attccaaatc gtgcccttgg
     1081 acataccgga gatggcaaag agttttccta ttctgctccc atgagcatga cgttgccata
     1141 tgcggcaggt gcgttagagt caaatgttga cgatctggcc atttggaacc acgcaatggc
     1201 ttctggaaaa ttgctgaaac cagccacctg gaaacgagct tttaccccct acaccctcag
     1261 taccgggaag ccaactacgt atggctatgg cctggaattt gaacaacttc agggagaaga
     1321 ggtgattgaa cacggcggcg gcatcaacgg ctttatcacc atggggtact ggctccctaa
     1381 acaaaaggtg tttgttgcta tcttgacaaa tcaggatagc gccgaagttc agccgtcatt
     1441 tgtggcccgt aaggtggctg cgacgatgat tggtaagggg tttaaggaat atactgcagt
     1501 tacactggat gccaaaaagc ttgatgaatt cgttggggtg tatttgattg aggacaagac
     1561 cacccggacc atcacccgtc agggaaatca actgtatgct cagcgcaccg gcgggcagaa
     1621 aatggaaatc ttcccagctt ctgacaatga gtttttttat aaaagctcgt tcacggtact
     1681 caaatttgtt cgggatgaag ctggcaagat cgtcaaaatg atcaaaactg acgaaaaagg
     1741 tgaagaatcc gctatcaaaa cagaccagcc agctccgaaa gagcgtgaag tcgccaaagt
     1801 ggatccagcc atctatgacg cttatgttgg tgaatatgaa ctggtacccg ggtttgtgct
     1861 gaccgtaaca cgggacggtg acaaactctt gacccaggcc accgggcaac agcggctaga
     1921 gattttccct gagtcagaaa ccgaattttt cttaaaagtg gtggatgccc aactgacctt
     1981 cgtcaaagat gcagacggga aagtcaatca ggtgattctc catcaaggcg gacggaatat
     2041 gcccgcaaag cgaattaagt aacgctgaag acaagggcga gtccctgggt acaatcctct
     2101 caggcgcggt gcagaatgtt ctgcgccgcg cttttgtctg tctgagtgaa attcgggaaa
     2161 gtttcgggag gggttatggc tggtagaaaa aacggaatgc tagtatcgcc cccatgtctg
     2221 aatctatcct cagttccatt ccaaaatatg aagttgtggt ggttggcgct ggcatagccg
     2281 ggttaacgac agcagctttg ctccaaaaag aaggaattca aacacttttg cttgaagccc
     2341 acgataaagt tggtggctgt gccgggtatt tttttcacaa gggatacacg tttgatgcgg
     2401 gagccactgt catcattgga cttgatccag aagggctaca ttcctatgta tttcaacagc
     2461 ttggagtcgc agtgcctgaa tccactttga ttgaatgcgt gacggttcat cttcctgacc
     2521 gaacagtgaa cctgtggcac gatcaaggga aatgggagcg cgagcgagtc gaacgatttg
     2581 cccgcacccc tgatgaagcc cgccgactgg ctgccttttg gaaacaggtg gatacgacgg
     2641 ctgatgtgct ctggcaaatg acggcaaagc agccggtgtt gccagttcga ggcattcgag
     2701 atttgatctc caatctgcgc ctggtttcgc ctgatgtgct gtggattttg ccagccgcca
     2761 tctccacgtt tgatgacatt ctgcgccgtt atcaattgag tgatcatgct gaactccgag
     2821 cttttctgga tggcttgctc ctgattacca cgcaggaaac tgccgagcgg gcaccgtttt
     2881 ataatgctgc cgctggcatt gatctctatc ggcatggaat tcggcgagcc agaggcggga
     2941 tgaaaacact ggttcgaaca ctcctggaag cctatcgaag ccatggcggc accatcaaat
     3001 ttcgtcagaa agtcaccgaa attgagcctc ggtctcaagg cggatatgtg gtgaaaactc
     3061 gtgaagggct gatcgttgag gcgaaaaagg tggtcgccaa cattccggtt tggaatgtcc
     3121 cgcagcttgt caaagccgat cttactgcca gtttttcccg tgctctgaaa aaagctgata
     3181 ttggttgggg ggcttttacc ctgtatctcg gagttgatga gtcggttatt cccccagaca
     3241 gtgcactcaa tcatcaggtg ttgcaacgtt atggcgagcc atttaaggat gccaatagtt
     3301 gctttctctc gctgagtgaa gctggtgatc tgcgttcagc cccggcgggg aaacgaacgc
     3361 ttgtggtctc gacccacacg gaagtcaaca cctggtatgg tctctcactc gaagactata
     3421 cccggcgcaa acaggcgttg accgaccaga cgctggcagc catccgaaaa gtctttccag
     3481 atattgatcg tgggcttgaa gttgtgcttc cggggacgcc agtcaccttt gaaaattaca
     3541 ccagccgcga gcgagggatg gttggcggat tgcgaactca gcttaccaat agcaacctgc
     3601 tggcgatttc gtcagatatt ggactcaaag atttctggct ggtcggggat accacgtttc
     3661 cagggcaggg aacaatggcc tgtaccttga gcggaatcaa tgcctggcgg gatatttgtg
     3721 gaaagctgaa gaaggtgggc tgaagacatc gggctgaaaa tactcgggct gaaaatactc
     3781 gggctgaaaa tactcgggct gaa
//
LOCUS       DAOQDO010000307         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17254_length_3803_cov_2.725720, whole genome shotgun sequence.
ACCESSION   DAOQDO010000307 DAOQDO010000000
VERSION     DAOQDO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17254_length_3803_cov_2.725720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            635..2374
                     /locus_tag="PKZ53_06265"
     CDS             635..2374
                     /locus_tag="PKZ53_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40075.1"
                     /translation="MSTVPVFEAEREASLQVSSHVQVFEGEIAIAEEADLFLKRGEFT
                     REELIERHRFLIKKYRSLMKQATKIVKVGDSTQARLIRTQHELDKQNEELAKTVEQLR
                     ISEQKALEANRAKSTFLANMSHELRTPLNAILGFVQVMQRRQGRDTEDTENLQTIMRS
                     GEHLLGLINDVLSISKIEAGKITLTEHTFHLPKLLLGLEEMIRVRAQSKGLQLSFEIS
                     PELPEYVLGDEGKLRQVLINLLGNAVKFTERGQVTLRVFWSNSIATFEIEDTGHGISK
                     EEQTKLFEPFVQTESGQKSTEGTGLGLAISRNFVNLMGGEIWVRSELGEGTTFCFTSR
                     LPQSSRESTAPILGKVIGLAGNQPAPRILIVDDKEENRQILCKLLSPVGFQVTEAING
                     VEAITQFKKHRPDLIFMDLRMPVLDGYEATKAIRQLESALLVESESEVPSTRTSIIAL
                     SAMAFEHDREAVHNAGCDGFLAKPFREEDCFEVLRQHLNLTFKYESAASPQTTAYASS
                     TAHQALTRERLAALPKSLQEELHQALIRGDTQIATDIARRVRQFDAPLAEELMNTVKT
                     YCFDEVLDLLETI"
     gene            2544..3191
                     /locus_tag="PKZ53_06270"
     CDS             2544..3191
                     /locus_tag="PKZ53_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ40076.1"
                     /translation="MLNRRIFLSYVACLLGFLSLATAVLTDSDKRFQIDITISQAVQS
                     ISLPGFETGMRLVSFPGNHALADLVWGCLILTLLLWKRRKVAGYVFALSAGGGTLLNM
                     IVKELVARPRPTSDLVQQLVHETSLSFPSGHVMHYVTCYGFLMMLFYHEPLPQWLKIS
                     LTTLCGTLILLVGTSRVYLGAHWPSDVTAGYLLGGLWLGLCIWLYHQWQAKSNQE"
     gene            3465..>3803
                     /locus_tag="PKZ53_06275"
     CDS             3465..>3803
                     /locus_tag="PKZ53_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40077.1"
                     /translation="MWDHFLGWLLSWLLDLLGGIQLRLWIGFLMFLVVYTLFTFWSYR
                     FSIRSVGIREATYAQSICVRLVDLALFGLLFFTGWYFPLGIPLLVAARVISFMVFFKA
                     EFGKALLGVLL"
BASE COUNT          921 a          945 c          926 g         1011 t
ORIGIN      
        1 cgtttcctct tggccctcaa ggaccgggtc tccgcgccga agataggatg aaaagtgaag
       61 aaccgtttag tggttggtgg ttagcggtta gttcttagtt cttagtcctt ggttcttctt
      121 cgaaagtatt gatttccagc cactaaccac caactccatt ttccattccc caaatgtcgc
      181 cccaaatact gactgcttcc tcggttgtgg tacctgatga gaccacaatc aaaggacaca
      241 aaggacataa aggacggaaa ggaccggttc gaagtcggtt ttatcggagt gacgaggaaa
      301 atttggtgaa ttttcattcc tgtcaaagct taccgaaatt ccccggtgcg agtcagttcc
      361 cgaactggtc ggcgctgatg tttgagccag atggtgctct tttgggcaaa attgaggtca
      421 ttctttattc ttcattcttc attcttcatt cttcattctt cattcctgct tcattcttca
      481 ttcttcattc tcccttccca aattcctcta ttccctctca aaaaaaatct gtaaagtaac
      541 accgtctttt ccgcgagcaa gaccacccct aaaatcccaa aattcagatc caagggcatc
      601 cacaggtttt atgaacaagg aatttgaaga tagcttgtct accgttcctg tttttgaggc
      661 agaacgagaa gccagtctcc aggtgtcgtc gcatgtccag gtgtttgaag gggaaatcgc
      721 gattgctgag gaagccgacc tttttttgaa acgaggcgaa tttacccgtg aagaactgat
      781 cgaacgccat cgctttctga tcaagaaata ccgcagcctg atgaagcagg ccaccaagat
      841 cgtaaaagtt ggcgacagta ctcaggcccg gttgatccgg acccaacacg aacttgataa
      901 acaaaatgaa gaactggcca aaactgtcga acaattgcgg atttctgagc aaaaagccct
      961 tgaagccaat cgtgccaaaa gtacctttct ggcgaacatg agccatgaac tgcgaacgcc
     1021 gctcaatgcg attttaggat ttgtccaggt gatgcaacgc cgccagggtc gggatacgga
     1081 agatactgaa aacctgcaaa cgattatgcg gagcggtgaa catctgttgg gactgattaa
     1141 cgatgtcctg tcgatttcaa agattgaagc cggaaaaatc accctgacgg aacatacctt
     1201 tcacctgccg aagttgctcc tgggccttga agaaatgatc cgggtcagag cccaatccaa
     1261 ggggctccag ttgagctttg aaatctcgcc cgaattgccc gaatacgtgc tcggcgatga
     1321 aggcaaactc cgtcaggtgc tgatcaactt gctgggcaat gcggtcaaat ttactgaacg
     1381 cggacaggtg accctgcggg tgttctggtc aaacagcatc gctacctttg agattgaaga
     1441 caccggccac ggtatctcaa aagaagaaca aaccaagctt tttgaaccat ttgtacaaac
     1501 tgagagcggt cagaagtcaa ccgaaggcac gggtctgggt ctggcgatta gccgcaattt
     1561 tgtaaatttg atgggtggcg aaatctgggt ccggagcgag cttggtgaag gcacgacgtt
     1621 ttgctttacc tcccgcctgc cacagagttc acgtgaatca accgcgccga tactcggaaa
     1681 agtgatcggc ctggcaggta accagccagc gccgcgcatt ttgattgtag atgacaaaga
     1741 agagaaccgg cagattcttt gcaaattact cagtccagtt ggctttcagg tcaccgaagc
     1801 catcaatggc gtcgaggcca tcacccagtt caagaaacat cgcccagatt tgattttcat
     1861 ggatctgcga atgccagttc tggatggata cgaagcgacc aaagcgattc gtcagcttga
     1921 gtcagcgctt ttggttgaaa gcgaatcaga ggtgccgagt acccgcacca gtatcattgc
     1981 cctctcggca atggcctttg aacatgaccg ggaagccgtc cataacgccg ggtgtgatgg
     2041 ctttttagcc aaaccatttc gtgaagaaga ttgttttgaa gtgctgcgcc agcatctcaa
     2101 ccttaccttc aaatatgaat cggcggcctc accacagaca accgcctatg cttcctccac
     2161 cgcccaccag gcactcacca gagaacgtct ggccgccttg ccgaaatcgc ttcaagaaga
     2221 attgcatcag gcattgattc ggggcgacac ccagattgca actgacattg cccgtcgggt
     2281 tcgtcaattt gatgcgccgc tggcagaaga actgatgaac accgttaaaa cctattgctt
     2341 tgatgaagtg ttggatttgc tggaaaccat ttaagggcaa ggcgtaagga gcgagtggag
     2401 gagtcagaaa aagtccagga attgttcatc tggtaacttt cgtttttgga agggtgaaca
     2461 gtttgtgccg cttgtatttg gattattgat gacttgtact cgctcctcac ttttgtttcg
     2521 cgacttcgct cattattcaa acaatgctaa accgccggat tttcctttcc tatgtcgcct
     2581 gtttgctcgg ctttttaagc ctggcaaccg ctgttttgac cgattccgac aaacgctttc
     2641 aaatcgacat caccatctct caagccgtgc aatccatttc cttacctgga tttgaaacag
     2701 gaatgcgcct cgtttcattt cccggaaatc atgccctggc agatctggtc tggggatgcc
     2761 tgatccttac attgttactc tggaaacgcc ggaaagtagc cggatatgtg tttgcgcttt
     2821 ccgcaggcgg ggggacgctc ctgaacatga tcgtgaaaga actggttgcc cggcctcgcc
     2881 ccaccagcga cctcgttcaa caactggttc acgaaacttc gctgagtttt ccatcaggcc
     2941 atgttatgca ctatgtcacc tgttatggat ttctgatgat gcttttctat cacgagccgc
     3001 ttccgcaatg gctcaaaatc agtttgacca ctttgtgtgg aaccctgatc ctgctcgttg
     3061 ggacatcccg agtctatttg ggggcacact ggccaagcga tgtcaccgcc ggatatttgc
     3121 ttggaggact gtggctgggt ttgtgcatct ggctctacca tcagtggcag gcaaagtcga
     3181 atcaagaatg aagaatgaag aattgagaat gaagaatgaa gaatgaagaa aattcttgat
     3241 gtctggggcc tggtttcgaa aaccccgaac cccaaatcct gacagatgac caactgacaa
     3301 ggtgacaagg tgatttcttt cagccctcag ccctcagccc ttccgaaaac ccggaacccg
     3361 gttcttcatt cttacagggt taaacccttg cgggttcagt ggtagccata atatgctgaa
     3421 ccctttccaa ctcgcctcat gctttaaccg aaaggaatcc gtgtatgtgg gatcattttc
     3481 tgggctggct tctttcctgg cttttggacc tgctgggtgg gatacaactg cgattgtgga
     3541 ttggcttttt gatgtttctg gtggtctaca ccctgttcac cttctggtct taccggtttt
     3601 cgatccgctc agtcgggatt cgtgaagcca cctacgccca atcaatctgt gtccggctgg
     3661 ttgacctggc gttgtttgga ttgctgtttt ttactggctg gtatttcccg ttggggatcc
     3721 cgcttctggt ggctgcccgc gtcatcagct ttatggtctt ttttaaagct gaatttggta
     3781 aagccctgct tggcgtattg ttg
//
LOCUS       DAOQDO010000308         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17326_length_3794_cov_2.956940, whole genome shotgun sequence.
ACCESSION   DAOQDO010000308 DAOQDO010000000
VERSION     DAOQDO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17326_length_3794_cov_2.956940"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="tgt"
                     /locus_tag="PKZ53_06280"
     CDS             complement(<1..730)
                     /gene="tgt"
                     /locus_tag="PKZ53_06280"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNJ40078.1"
                     /translation="MSIQFELLAQDTQTNARAGILRTAASEIETPVFMPVGTLGSVKA
                     LTQDMLEEMDVRILLGNTYHLYLRPGHEIVRELGGLHSFMSWNRSILTDSGGFQVFSL
                     GKLRKITEEGVMFQSHLDGSRHLLSPEKSMEIQAALGSNIVMAFDECTPYPATREQVA
                     VSLELTARWAKRSREAFDRLADRERQALFGIVQGGVHPDLRLRSLESLVEIGFEGYAI
                     GGLSVGESKEQMYDTTEFIAPRLPA"
     gene            888..1868
                     /locus_tag="PKZ53_06285"
     CDS             888..1868
                     /locus_tag="PKZ53_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ40079.1"
                     /translation="MRVSRLLQLSLGIGFALLVSLPHLAQGPQLPRPQVESTNSDTIL
                     RINSELVSVDVTVVDERGEYVHGLSATDFSLFEDGKPYPIEFFQPNSTQGPRLVSMVF
                     AVDFSGSVTLEEGATQQRALSTFLNDQNPNSLFALIGFNDEVQVLENFTKDRKRLLRA
                     FEKQKEYGGSTRIYDAIDRGITLLKKSPRKYGERLFRRYIVVLTDGFDSSSMVNSREV
                     IRRANEEGISIYTVTVPSYTVSLNGRQRVPTLLDATRIATSTGGRDFCVEAGFDYTSA
                     FRAISAEVIESYTIAYQPSPESSPPQFRKLSVTTRHPHLTLRLSRQGVTR"
     gene            2019..3098
                     /locus_tag="PKZ53_06290"
     CDS             2019..3098
                     /locus_tag="PKZ53_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNJ40080.1"
                     /translation="MEKKVLDRILTMAVKSGVSDIHFQAGSVPLFRYNGALMDVKYDM
                     LTPRDTEAIARIILRMDRLHDYDDFVERDVSYGVEGAGRFRANIFKQRGSFGIVLRAI
                     PIEPRTIDQLNLPSTLVQIADLARGLILVTGATGNGKSTTMAGMIEHINRTRKVHVVT
                     IEDPIEVLYQNNKGVITQREIGQDTKSFSTAMHSALRQDPDVVLLGEIRDSETFDTAL
                     SAAETGHLVLSAIHTTDAPKTINRIVGFYPISEQPTVRARLTENMAAIISLRLLPMKN
                     GQGRIPAVEIMRMSPAIQDCVRNPEKASEIPGLIARSKDHYGMQTFDQHLVALYREGK
                     ISLEIARAAATSRGEFERAITLEGA"
     gene            3405..>3794
                     /locus_tag="PKZ53_06295"
     CDS             3405..>3794
                     /locus_tag="PKZ53_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNJ40081.1"
                     /translation="MTFSLLGMTCFGGGAVMGLIQDKVVRVKQWISNQEFIESVALGQ
                     SLPGPIGPNSIAHIGYRLQGMAGAAVSLVMFILPSFFLMVGFSIVYEYTRNVPAVSHL
                     FFGLNPAVTGLVAGTAVRLGTSALQTRY"
BASE COUNT          924 a          990 c          915 g          965 t
ORIGIN      
        1 cagcaggaag ccgtggagca ataaactcag tggtatcata catttgctct tttgactcgc
       61 caacactcag ccccccgatg gcataccctt caaatccaat ttcaaccaga ctttccaacg
      121 aacgcaaccg caagtcaggg tgaaccccac cctgaacaat gccaaacagg gcctggcgtt
      181 cgcggtcagc caggcgatca aacgcttcac gtgaacgttt ggcccagcgg gcggtcagtt
      241 caagtgaaac ggcaacctgt tcacgggtgg ccggataggg ggtgcattca tcaaacgcca
      301 tcacaatgtt tgagccaagc gccgcctgga tctccattga tttttcaggg ctaagcaagt
      361 gtcgagaccc gtcaaggtgt gattgaaaca tcaccccttc ctcggtaatt ttgcggagct
      421 tccccagcga aaaaacctga aatccaccgc tatcggttaa aatcgagcgg ttccagctca
      481 taaaagaatg caacccaccc aactcacgca cgatttcgtg tccaggacgc agataaagat
      541 gataggtgtt gcccagcagg atccgaacat ccatttcttc gagcatatcc tgggtcaggg
      601 ctttgacact ccccagcgta ccgacaggca taaacaccgg ggtttcaatt tcactcgcgg
      661 cggttcgcag tattccagcc cgggcattgg tttgtgtatc ttgagcaagg agttcaaact
      721 gaattgacat gataataagg agagaatttt tcgagattcg tcacaaacga aacaccatat
      781 cctgaactgt cgtttggagg caacattatc cttcctgttt caactcagct tccaaatcac
      841 aaaccacgtt catcagcttt tcgaaacgaa tttgaccaga ggtacttatg cgtgtgtcac
      901 ggctgctcca actctcactt ggcattgggt ttgcacttct cgtatcactt ccgcacctgg
      961 cccaggggcc gcaattaccc cggccccagg tggaatccac caactcagat actattttgc
     1021 ggatcaactc tgagcttgtc tcggtggatg taaccgttgt tgatgaacga ggagagtatg
     1081 tgcatgggct gagcgccact gatttctcgc tgtttgaaga cggcaagccc tatccaattg
     1141 aattttttca gccgaactca acccaggggc cgcgtctggt ttcaatggtt tttgcggttg
     1201 atttctcagg gagcgtgacg ctggaagaag gggccaccca acaacgagcc ctgagtacgt
     1261 ttttgaatga ccagaatcca aattctctgt ttgcactgat cgggtttaat gacgaagtgc
     1321 aggtcctgga aaattttacc aaagatcgga aacgtctgtt gcgggccttt gaaaaacaaa
     1381 aggaatatgg cggatcaacc cgcatttatg atgcgattga tcgggggatt accctcctca
     1441 aaaaatcacc ccgaaaatat ggcgaacggc tgtttcgtcg ctatattgtg gtcctcaccg
     1501 atgggtttga tagcagcagt atggtgaatt cccgcgaagt gatccggcgg gcaaatgaag
     1561 aagggattag catttacacc gtgaccgttc cttcctacac cgtcagcctg aatggacggc
     1621 agcgcgtacc aacgctgctt gatgcgacgc ggattgcaac ttcaacaggt ggccgcgatt
     1681 tttgtgtcga agccggcttt gattacacca gcgcctttcg tgcgatttct gccgaagtca
     1741 ttgaaagcta taccattgcc taccagccat cacctgagtc atcaccacct caattccgaa
     1801 aattgagcgt cacgacccgc catccacatt taacacttcg gctgagtcgg cagggagtga
     1861 caaggtgacc gtgtcatttt gtcacgagtg cattctcacg cttgtgactt gtcttcaacc
     1921 cgacgaatat gacaaattat ggatgcacgc catctaaacg ctcttccagg tcaactcaca
     1981 caggaagtcc ctcagattaa actaaggata gttcacatat ggagaagaag gtactcgacc
     2041 gcattctcac catggccgtc aaaagcggcg tctcggatat tcactttcaa gctggaagcg
     2101 ttcctttgtt tcggtacaac ggtgctttga tggatgtgaa atatgacatg ctgacgcccc
     2161 gtgataccga agccattgcc cgaatcattc tccgcatgga ccgcctgcac gattacgacg
     2221 atttcgtaga gcgtgatgtc tcgtatggag ttgaaggggc tggccgattc cgggccaata
     2281 tctttaagca acgcggcagt tttggcattg tcttacgggc cattccgatt gaaccgcgca
     2341 ccattgacca gctcaatctg ccgtcgaccc tggttcaaat tgctgatctg gcgcgtggat
     2401 tgattctggt gaccggggcc actggaaatg gaaaatcaac cacgatggcc gggatgattg
     2461 aacatatcaa tcgaacccga aaagtccatg tcgtcacaat tgaagatcca attgaagttc
     2521 tgtaccaaaa caacaaaggc gtcatcaccc agcgagaaat tggtcaagat accaaatcgt
     2581 ttagtacggc gatgcactcg gcgcttcgtc aggacccgga tgtggtgttg ctgggtgaaa
     2641 ttcgggattc tgaaacgttt gatacggccc tctcggcggc tgaaactggt cacctggtcc
     2701 tgagtgcaat tcacaccact gatgccccaa agaccattaa ccgaatcgtt ggattttatc
     2761 caattagcga acagccaacc gttcgtgccc gactgacaga aaatatggcc gcgatcatct
     2821 cgttgcgtct gcttccgatg aaaaatgggc agggacggat tccagcggtt gaaatcatgc
     2881 gcatgtcacc agccatccag gactgtgttc gcaatccgga aaaagcctct gaaatcccag
     2941 ggctaattgc tcgcagcaag gatcactacg ggatgcagac ctttgaccag caccttgtgg
     3001 cactctatcg cgaagggaaa atcagtcttg aaatcgcccg agcagcggct accagtcgtg
     3061 gcgaatttga gcgtgcaatt acgcttgaag gcgcttagga gtgaagaatg aagaattgag
     3121 aatgaagaat gaagaattgg gaatgaagaa atttcctggt ttttggcgcc tggttctttt
     3181 tccctttcag cccttcgccc tggttttggc ttcttcattc tcaatgcttc atttgccttc
     3241 tgttttgttt cttcattctt cattctcaat tcttcattca agcggttctt cctatggcag
     3301 atggtaattt tcaagttgaa ccgtcagaat cacggtttct ccccacctct tcccctctct
     3361 ttccgcctcc ccccccttca caagttactc tcagtgatct ggctttgacg tttagcctcc
     3421 ttggcatgac ctgctttggc ggtggtgccg taatgggctt aatccaggat aaagtcgtca
     3481 gggtcaagca atggatttca aatcaggaat ttattgaatc ggtcgcactt ggtcagagcc
     3541 tgcccggccc aattggaccc aattcaattg ctcatattgg gtatcgcctt cagggtatgg
     3601 ccggggcggc agtatcgctg gttatgttta tcctgccatc gtttttcctc atggttggat
     3661 tttcaatcgt gtacgaatac accagaaatg tcccggcggt cagtcatctc ttctttggat
     3721 taaacccggc ggtgactgga cttgtagctg gcaccgctgt ccgcctgggg acttcagccc
     3781 tccaaacccg atat
//
LOCUS       DAOQDO010000309         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17331_length_3793_cov_3.898876, whole genome shotgun sequence.
ACCESSION   DAOQDO010000309 DAOQDO010000000
VERSION     DAOQDO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17331_length_3793_cov_3.898876"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..>3793
                     /locus_tag="PKZ53_06300"
     CDS             431..>3793
                     /locus_tag="PKZ53_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ40082.1"
                     /translation="MRSGTLTFILVVGLYFLGGGVLSSPTGSIIPDPQPAQTSASLPD
                     SKLSVSPAPAAIPLSDLTSSVSSGADPTDRLRPGFRVYTDRDGLPQNTIMAMAVDHNG
                     YFWAGTQDGAAWYNGRSWTVVNLPNQNNSNYITDILVASDQSIWFAQFDGGLCRLKDG
                     QWTTFDTRSGLPSNQMRCLLETKSSTGTPILWAGTDGGGLAKFENGKWSVITTQDGLP
                     SNRVWTLLEMVEPSGTSILWAGTFGGGLARLVQNTWQTFDRTSGLPDNSIRDLLASVS
                     PTGQPILWVATDSGGLAKFENDTWTVVDTHSGLPSNRVDALAETIAKNGVRTLWIGTP
                     GGLVRSEPGKWMVYDINYGLPNNTIRSLFSFSGPTASSTLWIGTFGGLVRLEQGKWLA
                     CDTRTGLPDNGVMSLLESRNPDGSYSLWFGTFGGGLARFERGKWTIYNAQSALPDNIV
                     AALLSTPGPDGTPTIWVGTNRGLARLEKNQWTVFNTKNGLPGDPVQCLMATRAADGTP
                     VVWVGTRGGLAKFQNGQWDSSLTEGLPPLARHIECLFETTSKNGTRSLWAGTRSGGLA
                     RWENGEWTIQDARTSLGNNWVFHLMEMTNAEGKRQLWAATYGSGVLQLDLDTPNAKWQ
                     ALSDHQKQLKPYGVVYQMRRDIQNRIYLFTTKGIVRLTPRTPTPANPEAFDVNFFTIE
                     DGLPASGCTEGGSLIDHQGRIWAGTTGGAAVFDPALETLAQTSRQLFIERTLINGTEL
                     DQTKLAGVPPTSIGKAPALEGISLAYSDNTIMFEYSLLSYFRESETRYQTQLVNFDDS
                     PSEWKKEAQKEYTRLPEGHYTFKVWGKDYAGNISGPLLVSFRIRPAPWRTWWAYIAYT
                     ALILGSGYGWYSRRIRTLRRRQEEHLAHLQQLQEQRIKTLRQLLESIRIINSELDLTV
                     VLQNIAAESANLVDGRPGGIGLVEDEQVVFKRVWHRNHWEDCLFAYPMGAGIPGRVAA
                     TGQPTVLNDLSFPSDETNSPHHHLQTCSGLINVPILSRMGNVVGVLDVRRGPDRDPFT
                     EADCQLIESLAHQAAVAMEKAALFGELEKLYQQEQEVRQTLQDLNQMKTNFIIVTSHE
                     MRTPLTVLKGYNEALVDGYLGPLTKTQKTSLLT"
BASE COUNT          898 a         1032 c          976 g          887 t
ORIGIN      
        1 gcctggttct tggttcttgg ttcttggttc ttggttcttg gttcttggtt cttggaaggt
       61 attgggccca aagcactaaa taccaggtac cagatacgaa taccattttg tcatgaatgc
      121 ctcgcattag caaaaggcta agttattagg aaaattgaat ttccctgcct cctgaccccg
      181 agcccctgat cccaaaatgg tataaccact catcactaaa cactaaccac taaatggttt
      241 ctcaattctt cattctcaat tcttcattct tcattctcaa ttcttcattc ttcattctca
      301 attcttcatt cttcattctt cattcttcat tcgtaagtgg attcagagac ctttttcctg
      361 tatggtttgg cttcaggtgt gagtcatcct gccaacccta tcaaccaaag tagccaattg
      421 aatccagctt atgcgatcag gaacactcac cttcatcctg gtggttggtc tgtacttcct
      481 tggtggcgga gtcttgtctt ccccaaccgg tagcatcatt ccagatcccc aaccagcaca
      541 gacctcggcc tcgcttcctg attccaaact atcagtttcc cctgcccctg cagccattcc
      601 gttgtcagat ttgacatctt ccgtcagctc gggtgcggat ccaacagatc gcctccggcc
      661 tgggtttcgt gtgtacaccg accgggatgg gcttccccaa aacacgatta tggcaatggc
      721 ggttgatcac aacggctatt tctgggctgg tactcaggac ggggcagcct ggtacaacgg
      781 acgttcctgg accgtggtca acctgcccaa ccagaacaat tcaaactata tcactgacat
      841 tttggtcgcc tccgaccaaa gtatctggtt tgctcagttt gacggcggac tgtgtcgctt
      901 aaaagatgga caatggacca cttttgacac ccggtcaggc ttgccaagta accagatgcg
      961 gtgcttactt gagaccaaat cctcgacggg aacgccgatt ctatgggcag gcacggatgg
     1021 tggtggcctg gcaaaattcg aaaatgggaa atggtcggtg ataacgactc aggatggcct
     1081 gccaagcaat cgagtatgga ccttgctgga aatggttgaa ccatccggca cctccattct
     1141 ctgggctggg acatttggcg gcggcctggc ccggctggtg caaaacacct ggcaaacatt
     1201 tgaccgtacc tcagggctgc cagataactc aatccgcgat ttactggcca gcgtctcgcc
     1261 aaccggtcaa cccatcctgt gggtggcaac cgattcggga ggactggcca aatttgaaaa
     1321 tgatacctgg acagtggtgg atacccactc tggactaccg agcaaccggg tggatgcact
     1381 cgccgagacg attgccaaaa atggggtacg aacactctgg atcggtacgc caggtggact
     1441 ggtgcggagt gagcctggaa agtggatggt ttacgacatc aactatgggc ttccgaacaa
     1501 taccatccgt agcctgtttt ccttttccgg accaactgct tcctcaacac tgtggattgg
     1561 cacctttgga gggctggttc gcctcgaaca gggaaaatgg ctcgcctgtg atactcgcac
     1621 gggattgccc gacaacggtg tgatgagttt acttgagtcc cgaaacccag atgggagtta
     1681 cagtctgtgg tttgggacgt tcgggggtgg actggcccgg tttgaacgtg gcaagtggac
     1741 aatctataac gctcaatctg cattgccgga caatatcgtc gcggccctgc tcagtacgcc
     1801 tggcccggac ggaacgccaa ccatttgggt tggcaccaat cgtggactgg cccggctcga
     1861 gaaaaaccag tggacggttt tcaatacgaa aaacggcctg ccgggcgacc cggtccaatg
     1921 cctgatggcc accagggctg ccgatggcac acccgttgtg tgggttggaa cgcggggtgg
     1981 gctggccaaa ttccagaatg ggcaatggga ttccagtttg acagaaggtc tcccgccgct
     2041 ggctcgccat attgagtgcc tgtttgaaac cacatcaaaa aatggaaccc ggtcactgtg
     2101 ggctggcacc cggagtggcg gactggctcg ctgggaaaat ggtgaatgga cgattcagga
     2161 tgcacgcacc agcctgggta acaactgggt gtttcacctg atggaaatga ccaatgccga
     2221 aggtaaacgc caactctggg ccgcaaccta tggcagcggc gtcctgcagc ttgaccttga
     2281 taccccaaat gccaaatggc aggcattgtc cgatcaccaa aagcaactca aaccctatgg
     2341 cgtggtttat caaatgcgcc gcgacatcca gaatcggatc tatctcttta cgacgaaagg
     2401 gattgtccgg ctgacacctc gaactccgac tccagccaat ccagaagcat ttgatgtcaa
     2461 ttttttcacg attgaggatg gcctgccggc cagcggctgt actgaaggcg gctcgctgat
     2521 agatcatcaa ggacgaattt gggcgggcac cactggcggc gccgctgtct ttgacccggc
     2581 gctggaaacg ctggctcaaa cctcccggca gttgtttatt gaacgcacac tcatcaatgg
     2641 aactgaactt gatcagacca aactggctgg tgtcccccca acatccattg gaaaagcacc
     2701 agcactcgaa ggaatatcgc tggcttatag cgataacacc atcatgtttg agtattcctt
     2761 gttgagctat tttcgtgaat ctgaaacccg gtaccagacg caactcgtca actttgatga
     2821 ctcgccatcc gaatggaaaa aagaagccca gaaagaatat acccggctgc ccgaaggcca
     2881 ttacaccttc aaagtctggg gcaaagacta tgccggcaat atcagcggcc cgctgctggt
     2941 ttcatttcga atccgacccg ctccctggcg aacctggtgg gcctatatcg cctataccgc
     3001 actgattctg ggatccggat acggctggta ttcacgccgg attcgaaccc tgcgccggcg
     3061 ccaggaagaa catcttgctc acctgcaaca attacaggag cagcgcatta aaacgctccg
     3121 ccagcttctg gaaagtatcc gcatcattaa ttctgaactg gatctgaccg tcgtgctcca
     3181 aaacattgcc gccgagagcg ccaatctggt ggatggccgg cctggtggga ttggactcgt
     3241 ggaagatgaa caggttgtct tcaaacgggt gtggcatcgc aaccactggg aagactgcct
     3301 gtttgcgtat ccgatggggg caggcattcc tggccgcgtg gcagccactg gccaaccaac
     3361 tgtgttaaat gatttgtcgt tcccatctga cgaaacaaac tcgccgcacc atcacctgca
     3421 aacctgttca ggactgatca atgttccgat tttgtcgcgg atgggcaatg tggttggcgt
     3481 tttggatgtc cgtcgtggcc cggatcgcga cccgtttacc gaagctgact gccagttgat
     3541 tgaatcactg gcacatcagg cagccgtggc catggagaaa gcggcactct ttggtgaact
     3601 ggaaaaactc tatcaacagg aacaggaagt ccgtcagaca ttgcaagacc tcaatcagat
     3661 gaagaccaac ttcatcattg tcacctcaca cgaaatgcgg acgccgctga cagtcctcaa
     3721 aggctataac gaagcactgg tagacgggta cctcgggccg ctgaccaaga cccagaaaac
     3781 atcactgttg acc
//
LOCUS       DAOQDO010000310         3791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17356_length_3791_cov_3.170236, whole genome shotgun sequence.
ACCESSION   DAOQDO010000310 DAOQDO010000000
VERSION     DAOQDO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17356_length_3791_cov_3.170236"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKZ53_06305"
     CDS             complement(<1..730)
                     /locus_tag="PKZ53_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40083.1"
                     /translation="MLSLTLTFRRLSAATLSALLLGGSSLPALAQNPNPTPESLVAVN
                     TPTDGIVRTQAPATSTSTQDATSSAKQEPKAVDTLPTERVGVDQSKVLRLALHDAILM
                     ALNSNIDIEVERGNVQIAQYDMDGSRGAYDPLLSSQFNFNSSVTPETRIFVGAQGGSV
                     ASKQLDYNAAFRQALTTGGNYQISFNNSRQETNAQSQGLNPQYFSELRFQFTQPLMRN
                     YRSSQNERLIKINKKQLTLSDSAFR"
     gene            complement(838..1191)
                     /locus_tag="PKZ53_06310"
     CDS             complement(838..1191)
                     /locus_tag="PKZ53_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNJ40084.1"
                     /translation="MNKLATVENPYAVLGIKRDASEVEIKQAYFAKVREHSPERDPEG
                     FKRIRAAYDKIRSAKTRAETDLFLVESPTLADFRAQFLRTSVPLPELTPELIKADLLA
                     LETTLIYEEVVTSLQ"
     gene            complement(1294..3606)
                     /locus_tag="PKZ53_06315"
     CDS             complement(1294..3606)
                     /locus_tag="PKZ53_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40085.1"
                     /translation="MGKKHHPKNKPAPTVDLSEVLNQIQVRLADNQSEEALKGLRRLE
                     TQLRQAEAKPGSADPALIAAKPQIHTLLAKANFASALQTTDPVQKNAFLDEALKRSPA
                     DAGYLFTAGATALVQGNPKLALDRFQKVHQLAPGNRLLERAQLTAWLVAGQTREVRDV
                     LRRLTPEQTTPAHQRIEFLRRALTGGLEPTATQPAKPVQAKKSKSTADTPFSALETPV
                     FSGWTALLQGNEPEALTQFTNLPVVTHSLSGAEAAVLGSQSFYAGWMQLRAGELKSTL
                     MSLSEAHRIASAAGLTLPWVTRLKPCFFALADQALQTDQLDIAVAAWEVLLQLEPANE
                     AVKKNLALAFQIKAGDAWLKQNLPEAIELWKKSYDGNRNEQTLKHLAIALEQSEKIDE
                     SISYWKQYLQRQRQQLKSRSADTAFKKYLVQLTEHVLDLMMTVDRPNHEVFQELDNGL
                     KLDPTNLELRKIGAEIYMGLGKTQQALKHIEVIERAHGDSVELLILKGFILGPEKKNF
                     DKSFACFEQAYQLDPSNPGAQAVYALGLNRVGINAEESGDTQTAIEFYEKAIAINPSF
                     TLSLTSLLLLYLTQQDQKKVDETIARLLASEGDKVGIHLSIGSALLNHGDKKQAETHL
                     KQALKLDPSADTIQALGEIYFEAEDMKEAHKYFDRLLKEDDIEPALNVVSLLGKEGLY
                     REALEYSDKVARAFPLDPEPHFAKAFYLCQQMDLTNAYAELEAAERKALKYDPLDRLP
                     EIRMQKNEIQRVLDLMKRLPPGGPKFPFGF"
BASE COUNT          854 a          880 c         1013 g         1044 t
ORIGIN      
        1 ggcggaaagc actatcactg agcgtcaatt gctttttgtt gatcttaatc aaccgttcgt
       61 tctggctgga acgatagttg cgcatcaacg gctgggtaaa ctggaaacgc aactctgaaa
      121 agtattgcgg attgagtccc tgagattgag cgttggtttc ctggcgtgag ttgttaaagc
      181 tgatctggta gtttccaccc gttgtgagtg cttgacgaaa ggccgcattg taatcgagtt
      241 gtttgctggc gaccgatcca ccttgcgccc ccacgaagat tcgggtttcc ggtgtcaccg
      301 acgagttgaa attgaattgc gagctaagca atggatcata ggcgccgcgg gagccgtcca
      361 tatcatactg ggcaatctga acgttgccgc gctcaacttc gatatcaata ttgctattga
      421 gcgccatcaa aattgcatcg tggagggcca gtctgagcac tttgctttgg tccaccccaa
      481 cgcgttcggt gggcagcgta tcaaccgctt tgggctcttg tttggcagag ctggttgcat
      541 cctgggtgga agttgatgtg gcaggtgctt gcgttcgaac aatgccatca gttggggtgt
      601 tgacggcaac cagcgattcc ggtgtcggat tgggattctg ggccagggca ggaagcgagc
      661 ttcctccgag caacagggct gagagcgtcg cagcactcaa gcgacgaaac gtaagggtca
      721 gactaagcat cagtttgctc tcctcgaaag atgaatccgt ttgattttag atgatttgaa
      781 aagcaggtgt tttggaattg ccgccagata cgtcttcaat tttgggatag tttcaatcta
      841 ttgaagactc gttacaactt cttcataaat caaagtggtt tccaacgcca gcaaatcggc
      901 cttgatcaac tctggtgtca gttctggcaa tggtaccgaa gtgcgaagaa actgtgcgcg
      961 gaaatcagcc agcgtgggtg attcaaccag aaacaggtcg gtttcggcac gggttttggc
     1021 agatcgaatt ttgtcatagg cggcccggat tcgcttaaac ccttcaggat cacgctcagg
     1081 tgaatgctcg cgcactttag caaaatatgc ctgtttaatt tccacttcgc tggcatcacg
     1141 tttgatgccc agcaccgcgt atggattttc aaccgttgca agtttgttca tgcaaagact
     1201 ctctatgttc taattacgaa atacacgaaa aacaccaaac ggatcaagtg tttaccaggg
     1261 ctaattccac gacgaggcca atgattcggg tgcttaaaac ccaaatggga atttcggtcc
     1321 gcctggaggc aggcgtttca tcaggtccag cacccgttga atttcatttt tttgcatccg
     1381 gatttcaggt aatcggtcca acgggtcata tttcaatgct ttgcgttctg ctgcctcaag
     1441 ttcggcatat gcgttcgtca gatccatttg ctggcacaga taaaatgctt tggcaaagtg
     1501 aggttccggg tcaaggggga aggctctggc aactttgtcg gaatactcca gggcttcccg
     1561 gtacaaccct tctttcccaa gcagcgacac cacattgagc gccggttcga tatcatcttc
     1621 cttgagcagg cgatcaaaat acttgtgagc ttctttcatg tcctcagctt caaaataaat
     1681 ctcacccagg gcctgaatgg tgtcggcaga tggatccagt ttcaatgcct gtttcaagtg
     1741 ggtttcagcc tgttttttgt cgccatgatt taagagcgca ctgccaatgg aaagatgaat
     1801 gccgacttta tctccctcgc ttgccagaag ccgggcgatg gtttcatcca cttttttctg
     1861 atcctgctga gtcagataga gtaagagcaa cgacgtcaac gagagggtaa aactagggtt
     1921 gatggcgatt gctttttcat aaaattcaat tgccgtttgg gtatcgccac tctcttcagc
     1981 attgattcca actcgattga gaccaagggc atagacggcc tgagcgccgg gattgctcgg
     2041 atccagttga taggcttgct caaaacaggc aaaggacttg tcaaaattct ttttctcagg
     2101 acctaaaata aatcccttta aaatcagaag ttcaaccgag tcaccgtgcg cccgttcaat
     2161 gacttcgatg tgtttgagtg cctgctgggt cttgccaagc cccatgtaga tttcggcccc
     2221 gattttgcgc aattcgaggt tggtcgggtc gagtttgaga ccgttatcga gttcttgaaa
     2281 cacttcgtgg ttggggcgat ctacggtcat catcaaatcg agcacatgct ccgtcaactg
     2341 gaccagatat ttcttaaatg ctgtatctgc cgatcttgat ttgagttgct ggcgttgtcg
     2401 ctgaagatat tgcttccagt atgaaattga ttcgtcaatt ttttctgatt gttcaagcgc
     2461 gatagccagg tgttttaacg tctgttcgtt tcggttccca tcataggact ttttccacaa
     2521 ttcgatggct tcgggtagat tttgcttgag ccaggcgtca ccagctttga tttgaaatgc
     2581 cagcgccagg ttctttttga cggcttcgtt ggcgggttcc agttggagga gcacttccca
     2641 ggctgccacg gcgatatcaa gttgatcggt ctggagtgcc tgatccgcca gggcaaagaa
     2701 gcagggtttg agacgagtga cccagggtaa agtcaatcca gcggcggagg caatgcggtg
     2761 ggcttcactc aaactcatca atgtcgattt gagttctcca gcccgaagct gcatccagcc
     2821 ggcataaaat gactggcttc ccagcaccgc agcttcagct ccgctcagag aatgcgtgac
     2881 aactggaagg ttggtaaatt gagtcagtgc ctcaggctca ttcccttgca gcagggctgt
     2941 ccagccagaa aacaccggcg tttcaagggc tgaaaagggc gtatcggcag tggatttcga
     3001 ttttttggcc tggactggtt tggctggctg ggtggctgtg ggttcgagcc cacccgtcag
     3061 ggctcttcgc aaaaattcaa tccgctggtg tgcaggtgtg gtttgttctg gtgtcaggcg
     3121 tctcaggacg tcacgaactt cacgggtttg accagccacc agccacgccg tcaattgggc
     3181 tcgctccagc aaccggtttc ccggtgcaag ctggtgcact ttctgaaagc ggtccagggc
     3241 cagtttggga tttccctgaa caagtgccgt ggctccagcc gtgaagagat acccggcatc
     3301 agccggcgac cgcttcaggg cttcatccag aaaggcattt ttttgcactg gatcagttgt
     3361 ctggagcgca ctggcaaaat tggctttggc cagcaacgtg tgaatctgtg gtttggcggc
     3421 aatcaacgcc ggatcagcac tgcctggttt ggcttctgcc tgccgaagct gggtttcgag
     3481 ccgccgcaac ccttttagtg cttcttctga ttggttgtcg gccagcctga cctgtatctg
     3541 attcaatact tccgaaagat caactgttgg tgccggttta tttttggggt ggtgtttttt
     3601 tcccataaat cacaaattga agaattgaga atgaagaatg aagaatgaag aatgaagaat
     3661 tgagaattgt cgggttcagg atttttgaat ttgggttgac accagtcttt gccgccgtag
     3721 cggtggttga attgagacag ttcagttagg gtttgtaaaa ttgaattggc gatgatttag
     3781 gattttgggt t
//
LOCUS       DAOQDO010000311         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17372_length_3788_cov_2.702384, whole genome shotgun sequence.
ACCESSION   DAOQDO010000311 DAOQDO010000000
VERSION     DAOQDO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17372_length_3788_cov_2.702384"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKZ53_06320"
     CDS             complement(<1..393)
                     /locus_tag="PKZ53_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ40086.1"
                     /translation="MPDRTILIVDDEPKTVASIQLYLEHNGFQVLIARTGREALELAR
                     TAHPDLIVLDLMLPELDGMAVCRILRAESTVPIVMLTARTTEADTLHGLDLGADDYIA
                     KPFSPRELVARVRAVLRRTIQPLDPRPSV"
     gene            complement(674..2002)
                     /locus_tag="PKZ53_06325"
     CDS             complement(674..2002)
                     /locus_tag="PKZ53_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HNJ40087.1"
                     /translation="MASSTIRITNGTLLTSGLQHQVIKGDLYITDRRISHIGTVPETA
                     DETIDATGCVVMPGFIQSHIHLCQTLFRGSADDLELLDWLKLRIWPMEASHTPESLRA
                     SAQLSIAEMIRGGTTAALTMETVNHTEAALQVVEETGFRAVVGKCMMDKGTEVPVGLQ
                     EKTDASIAESIRLIEKWHGRDDGRVRMCFAPRFAVSCTDGLLREVGKLARERNLIIHT
                     HASENRDEVALVYSETGQRNIAYLDSVGLTGTHVGLAHCVWLDDTELDILARTQTHVL
                     HCPSSNLKLGSGVAPIVEMHERGIRVSIGADGAPCNNRLDAFTEMRLAAILQKMRKGS
                     RHLAAAEAFRMATWEGARAIGQEDDLGSLEVGKRADVVIVNLNTLHAAPHPDPVSSLV
                     YSANASDVRDTIIDGQIVMRGRELKTLSEESVLHDVQREFESLLVRAGIA"
     gene            1980..2177
                     /locus_tag="PKZ53_06330"
     CDS             1980..2177
                     /locus_tag="PKZ53_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40088.1"
                     /translation="MRIVEEAMRNILWGRIGAEDIGLKIRKLRAEEVGRGPVLPVFFS
                     PIVFSPMSSTQSSSALVFQYL"
     gene            complement(2165..3010)
                     /locus_tag="PKZ53_06335"
     CDS             complement(2165..3010)
                     /locus_tag="PKZ53_06335"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNJ40089.1"
                     /translation="MVQTFAVIGAGTMGNGIAQVAARAGMNVILHDIAQAYLDRGLSA
                     IDSSLQRDVKKERLTVEGKQEILARIRPVTDLAALAEAEFVVEAVVENVDVKTKLFAQ
                     LDELTQPGVILASNTSSIAITRIGAATKRPEKVIGMHFMNPVPVMTLVEIIRGLATDD
                     ETNDQVTALCAKMGKTAISCQDSPGFISNRILMPMINEAIFALYEGVATPEAIDGIMK
                     LGMNHPMGPLTLADFIGLDVCLAIMEVLYDGFQDSKYRACPLLRQYVAAGWLGRKSGR
                     GFYKY"
     gene            3076..3657
                     /locus_tag="PKZ53_06340"
     CDS             3076..3657
                     /locus_tag="PKZ53_06340"
                     /inference="COORDINATES: protein motif:HMM:NF027615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HNJ40090.1"
                     /translation="MLIKMISCSVPREQRRAFEQAQERWRELQTVPGFGGQIGGWSLS
                     LKTHACILAFWKSQPLYQHFMAHDHDRILQISGQSGTYQSITVQFFEQKFDIPGHHDY
                     ILAALTAGTWLQITKLTLVDLAVTQFSDWSRDPDFLAGVAGCCPTATKDWLVATLWTK
                     PEPPDMSWNHLLPSETTTIALAPTWLVSSTDGG"
BASE COUNT          899 a          994 c         1002 g          893 t
ORIGIN      
        1 cactgacggg cggggatcaa gtggttgaat cgtccgcctg agtacagccc ggacgcgggc
       61 cactaactcg cgtgggctga aaggtttggc gatataatca tctgctccca gatcaagccc
      121 gtgcagggta tcagcttcgg ttgtccgggc ggtcagcatc acaatcggca cggtggactc
      181 agcccgcaag atccgacata cggccattcc atcgagttcc ggcaacatca aatcgagcac
      241 aatcaaatct ggatgcgctg tccgcgccag ttccagtgct tcgcgacccg tgcgagcaat
      301 caaaacctga aatccattgt gttccaggta aagctggatg gatgcgacgg ttttgggttc
      361 atcatcaacg atgagaatgg tgcggtcagg cataggagca gggctgaggg ctaggggcta
      421 agggttcagg gttcagggtt cagggttcag ggttcagggt ttcgaagttt tgtctttttc
      481 gtccttttct gatcaagaga cctggtttga caatgagtct cgtgaaaatt gtgaagaacc
      541 tgtgaagatc ggtttgtgag cagaaacaaa aattcaccac agaggccaca gagggtcaca
      601 gaggaattga ccaatttttg atcttcctct gtgtttctct gtggcctctg tggtgaaaaa
      661 agtgtttgcc aaatcaagca attccagccc gcacgagaag cgattcaaat tcacgctgta
      721 catcgtgcaa cacgctttct tcgctcaggg ttttgagttc gcggccacgc atgacaattt
      781 gtccatcaat aatggtgtcg cgaacgtcac tggcattggc tgaatacacc agcgatgaca
      841 ccgggtctgg atgcggcgcc gcgtggagtg tgttgaggtt gacgatcaca acatctgccc
      901 gtttgccgac ttcgaggctg cccagatcat cttcctgacc aatcgcccgg gcaccctccc
      961 aggtcgccat ccggaaagct tcagcggcgg ccagatggcg cgaacccttg cgcattttct
     1021 gcaaaatcgc ggccagtcgc atttcggtga aggcatcgag gcggttattg cacggcgctc
     1081 cgtcagcccc aatcgagacc cgaatgccac gttcgtgcat ttcgacaatc ggggccacgc
     1141 ccgaaccaag tttgagattt gacgatggac agtgcaacac atgggtctgt gtacgggcga
     1201 ggatgtcgag ttcggtatca tccagccaga cacagtgggc caacccaacg tgagtgccgg
     1261 tcagcccgac actatcaagg taggcaatgt tgcgctgacc agtttcagaa tacaccagcg
     1321 ccacttcgtc gcgattttcc gaagcgtggg tgtgaataat caaatttcgt tcacgcgcca
     1381 gtttcccaac ttcacgcagc agcccatcgg tgcacgaaac tgcaaagcgc ggggcaaagc
     1441 acattcgaac ccgcccatca tcccgcccgt gccatttttc aatcagacgg attgattctg
     1501 cgattgaagc atcagttttc tcttgaagcc caaccggaac ctcggttcct ttatccatca
     1561 tgcacttgcc aacgacagcc cgaaagccgg tttcttcaac tacttgcaag gccgcttcgg
     1621 tgtgattgac ggtttccatc gtcaaggcgg cggtggtgcc accccggatc atttccgcaa
     1681 ttgagagttg ggctgaagcc cggaggcttt ccggtgtgtg ggaggcttcc atcggccaga
     1741 ttcgcaattt caaccagtcc agcaattcca gatcatccgc cgaaccgcga aacagggtct
     1801 gacacaggtg aatgtgagac tgaataaaac ccggcatcac cacgcaacct gtggcatcaa
     1861 ttgtttcgtc tgccgtttcc ggaacggtac cgatatgact gatgcggcga tctgtgatgt
     1921 acagatctcc tttgatcact tgatgttgga ggccggaggt gagcaaggtg ccgttggtaa
     1981 tgcgaattgt cgaagaagcc atgcgaaata tcctttgggg aagaattggg gctgaagaca
     2041 ttgggctgaa aattcgcaag ctcagggctg aagaagttgg gcgtgggcct gttttacccg
     2101 ttttcttcag cccaatcgtc ttcagcccga tgtcttcaac ccaatcgtct tcagccctgg
     2161 tttttcagta tttataaaac ccacgaccgg atttgcgacc cagccagcca gcggcaacat
     2221 actgccggag cagtggacag gcgcgatatt ttgaatcctg gaagccgtca taaagcactt
     2281 ccataatcgc cagacacaca tccagcccga tgaagtcggc cagggtgagc gggcccatgg
     2341 ggtgattcat cccaagcttc ataatgccgt caatggcttc tggtgtcgca acgccttcat
     2401 agagcgcaaa aattgcctcg ttgatcatcg gcatcagaat gcggtttgaa ataaatccag
     2461 gtgagtcctg gcaggaaatg gcggttttgc ccattttggc gcacagggcc gtgacctgat
     2521 cgttggtctc atcatcggtg gccaatccac ggataatttc aaccagtgtc atcaccggca
     2581 ccgggttcat aaagtgcatt ccgatgactt tctccgggcg tttggtggcg gcaccaatcc
     2641 gggtaatggc gattgacgag gtattgctgg ccaaaatcac gccaggttgg gttaattcat
     2701 caagctgggc aaagagtttg gttttgacgt caacattttc gaccacggct tcaaccacaa
     2761 actcagcttc agccagagcg gccagatcag tcactggtcg aatgcgagcc aggatttcct
     2821 gtttgccttc gacagtgaga cgttcttttt tcacatcacg ctgcagactg gaatcaatcg
     2881 ccgaaaggcc gcgatccagg taagcttgcg caatgtcgtg caaaatcaca ttcattccgg
     2941 cacgagctgc aacctgggca atcccattgc ccatcgtgcc cgcaccaatc actgcaaatg
     3001 tttgaaccat aaaagatgat gactcctgtg tggattcagt aagaaaaagg gggagcaagt
     3061 gtagagaaga agcctatgct catcaagatg atttcttgtt cggttccacg tgaacaaaga
     3121 cgagcttttg aacaagcgca ggaacgctgg cgggaactgc aaactgtacc agggtttggt
     3181 ggccaaatcg gcggctggag tctgagtttg aaaacccatg cctgcattct ggctttttgg
     3241 aaaagccagc cattgtacca gcactttatg gcacatgacc acgaccgaat tttgcaaatc
     3301 agtggccaat ccggtaccta ccaatcaatc actgttcaat tttttgagca aaaattcgac
     3361 attccaggtc accatgacta tattctcgcg gcgctgactg ctggaacgtg gctgcagata
     3421 accaaattaa cactggttga tctggctgtg actcaatttt ctgactggag tcgtgatccg
     3481 gatttcctgg ctggggtggc tggttgttgc cccactgcta ctaaagattg gctggtggcc
     3541 acactgtgga ctaaaccgga accccctgat atgagctgga atcacctact tccgtccgaa
     3601 acaaccacca ttgccttggc gccaacctgg ctggtcagtt caacagatgg aggatgaaca
     3661 ttcccgctca gtcagtacca cccgcgtcag tgggtgggcc tgcttgcatc caaccccacc
     3721 cgctcacgca ggtggtactg acaagccatt tttgcattga gaggtgggtt atctacgaga
     3781 agttaaaa
//
LOCUS       DAOQDO010000312         3786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17388_length_3786_cov_2.374966, whole genome shotgun sequence.
ACCESSION   DAOQDO010000312 DAOQDO010000000
VERSION     DAOQDO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17388_length_3786_cov_2.374966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKZ53_06345"
     CDS             complement(<1..292)
                     /locus_tag="PKZ53_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40091.1"
                     /translation="MVRPHLILLLIFGLLTLVGQPCRAVSEPLKPLSSPTINTDLSAQ
                     CVPAIRVYNSQNGLPQSAVRALAFDQKGYLWVGTQDGTAYFNGQKWTTINVAQ"
     gene            695..2002
                     /locus_tag="PKZ53_06350"
     CDS             695..2002
                     /locus_tag="PKZ53_06350"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNJ40092.1"
                     /translation="MLSSNDVIVQTLGTPTIKSPLRLSTVRGDGMGNFVPDAEKIRFQ
                     VELQPGEVASDEIMFEKAGPRELIYFDPSDTRAAIVTCGGLCPGLNNVIRSVYLELYH
                     QYGVHTVYGIRFGYKGFNPASKPPVMLTRNFVESIHKRGGSVLGCSRGPEDTLVILDF
                     LEQQQINLLFCVGGDGTQRGAHAIAQEALRRGMKLAVVGIPKTIDNDIPFVYRSFGFS
                     TSIEKAKEVIDSAHEEANGAPNGISLVKLMGRHAGFITAAAAIASQEVNFVLVPEVPF
                     KLNGDKGFLECLKRRILNRGHAVIVVAEGAGQDLIASDIEGHDASGNVKLKDIGLYLK
                     EHINRYFEVQEIPINLKYFDPSYFIRSVPANCDDSLLCDQFARHAVHAAMAGKTNLLI
                     GLWYNMFIHLPISLATSKKKQISPESEFWMSALAATGQPAHFV"
     gene            2041..3213
                     /locus_tag="PKZ53_06355"
     CDS             2041..3213
                     /locus_tag="PKZ53_06355"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dispase autolysis-inducing protein"
                     /protein_id="HNJ40093.1"
                     /translation="MKKKLFFTAVVLSVLFGAHSTEPHIQAFVAQEHTAGQQNTAENP
                     ASSVPECNLVTGTAAVTFTTDNGKTLTPTEERLSGIGYTFGLTALRQPDTLLAVHNAT
                     LIRSTDAGCSWAKIADLNDEGDYFPLKLEASVGDRAYGWGDNRRYLVRIDGTDVTYLK
                     SPVDSIVGIGVDATNGDHLRAGGETGIWDSTDAGATWTRIGSLPVPNVSLILYRVAFD
                     PTNLDHILVGTAQTGAFFTTNGGQTWKSSKGLGAGKDAKINAFEFAFSPVRGKVVWAM
                     GLNLAESDAGQPSDGRHIYYSKNGGKSFKPVIDRDAEVTIRNQPVMKAHPTDPDVLYF
                     VFGTYFQGYGTDLFRFDVKSKKLDKTHNDYNDIDSIEFSRTDPSTMYLGLEVEQVQ"
     gene            3312..>3786
                     /locus_tag="PKZ53_06360"
     CDS             3312..>3786
                     /locus_tag="PKZ53_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40094.1"
                     /translation="MTSIAFITCDRIPNLTADDQLVVEELRKLGVGITPVVWTDNPDL
                     SGFELVVVRSPWDYHLKFDQFQNWLSAVEQDRIPLLNPAPIIKWNMNKQYLRELEARG
                     VPVVPSVWIQRGNTGPLQAVLDEQQWDQVVVKPTVSATAYETHRVTKQQTGKFQAQ"
BASE COUNT          919 a          923 c         1011 g          933 t
ORIGIN      
        1 gctgagcaac attgatggtg gtccatttct ggccattgaa ataggcagtt ccgtcctggg
       61 tgccgaccca gagataccct ttctgatcaa aagcaagcgc ccgcactgca ctctggggaa
      121 ggccattctg gctgttgtaa acccgaatgg ccggcacaca ctgggctgat aaatcggtat
      181 tgattgtcgg agaggaaaga ggtttcagcg gctcagaaac agcgcggcac ggctggccca
      241 ccaacgtgag caatccaaaa atcaggagca ggatgaggtg aggccgtacc atttagggtt
      301 caggggttcg gggttttcgc cgcggagcgg tggttgaatt gaggcaggtc gtttacggcc
      361 tgctcgtaat atggtgattt cattccaaac gatgggaggc aggagtttga agaaccaaaa
      421 ccacctaacc ctaacttatt caggaaatgg agtctatagc attgagaggc aaaaaaccgg
      481 ggacgggagc ggttttttga agtcgcagat ctgccaggga cttgcatcct ttgctacact
      541 acggcctgtc agtgccagtg ttgccccata caaccaggga tgaaggttga gggatgaaca
      601 gtaaagcggt caaagagttg atatttacga ggttaactca ttgagagcat tcactccccc
      661 tgaactctga gccctgaact cgaaaaggaa tcctatgctt tcttccaatg acgtgattgt
      721 ccaaaccctt ggaaccccaa ccatcaaatc tcctctcagg ctttcgaccg tgagaggtga
      781 tggaatggga aattttgttc cagatgccga aaaaatccga tttcaggttg agttacaacc
      841 cggcgaagtc gcttccgacg aaattatgtt tgaaaaggcc ggtccccgtg aactgatata
      901 ttttgatccg tctgatactc gtgcggccat tgtgacctgt ggtggcttgt gtccgggcct
      961 caacaatgtc attcggtcgg tctatcttga actctatcat cagtatggag tgcataccgt
     1021 gtatgggatt cgatttgggt acaaaggatt taacccggcc agcaaaccgc cggtgatgtt
     1081 gacccgcaat tttgttgagt ccattcataa acgcggtggg tcggttctgg ggtgctcgcg
     1141 tggaccggaa gacacgcttg tgattctcga ttttctagag cagcaacaaa tcaacctgct
     1201 cttttgtgtt ggtggtgatg gaacccagcg tggcgctcac gccattgctc aggaagccct
     1261 ccgccgagga atgaaactgg ccgtcgtcgg aattcccaag accattgata acgatattcc
     1321 ctttgtgtac cggagtttcg gattttcaac ttcgattgag aaagccaagg aagtgattga
     1381 tagcgcccac gaagaagcca atggcgcccc caatggcatc agtctggtca aattgatggg
     1441 gcgccacgcc gggttcatta ccgcggcggc ggcgattgcc agtcaggaag tcaattttgt
     1501 gctggtgccg gaagttccat ttaagctcaa tggtgacaaa gggttcctgg aatgcctgaa
     1561 acggcggatc ctcaatcgtg gccatgcggt cattgtggtt gctgagggcg ccgggcagga
     1621 cctgattgct tctgatattg aaggccacga tgcttcgggc aatgtcaaac tcaaggatat
     1681 tggcctgtat ctcaaagaac acatcaaccg gtactttgaa gtccaggaaa tcccaatcaa
     1741 cttaaagtac tttgacccaa gttattttat tcggagcgtt ccagccaact gcgatgacag
     1801 tttattgtgt gatcagtttg cccggcatgc ggtccatgca gccatggctg gaaaaaccaa
     1861 cctgctcatc ggcctctggt acaacatgtt tatccatctt ccgatttccc tggcaacgag
     1921 taagaaaaag cagatttctc cggaaagtga attttggatg tcggcacttg ctgcaacagg
     1981 tcaaccggct catttcgtat aggcccgaca tcccacctat ttttctggag atttcactac
     2041 atgaagaaga aattgttttt tacagcggtg gttctgtcgg tactttttgg tgcgcactcg
     2101 acagaacccc acattcaggc ttttgttgcg caagagcaca ctgcaggtca gcaaaacacc
     2161 gctgaaaatc cggcatcatc tgttcccgaa tgcaatctgg tcactggcac ggctgccgtg
     2221 acgtttacca ccgacaatgg aaaaacgctt actccgaccg aagaacggct gagtgggatt
     2281 ggttatacct ttggattaac cgctttgcgg caacctgaca cgttgctggc ggtgcataat
     2341 gccacgttga ttcgttcgac tgacgcgggc tgttcctggg caaaaattgc tgatctcaat
     2401 gatgaagggg actattttcc tttgaaactg gaagcttcag tcggagaccg cgcctacggg
     2461 tggggagaca accggcggta tctggtccgg attgatggaa cggacgtgac gtatttaaaa
     2521 tccccggttg attcaattgt tgggattggg gtagatgcaa ccaatggcga ccatctgcgg
     2581 gctggtggcg agactggaat ctgggattca actgatgccg gcgccacctg gacccgaatc
     2641 ggaagcctgc cggttccaaa cgtcagcctg attctgtacc gcgtggcgtt tgatccaacc
     2701 aacctggatc acattctggt tggcacagcc cagactgggg cgttctttac cacaaatggc
     2761 ggccagacct ggaaatcctc aaaagggctg ggcgctggaa aagacgcaaa aatcaatgcg
     2821 tttgaatttg cgttctctcc ggttcgtggc aaggtcgttt gggccatggg gctcaacctg
     2881 gctgaatcag acgccggtca gccttcagat ggacgtcaca tttactattc aaaaaatggc
     2941 ggcaagtcct ttaaaccagt cattgaccgt gatgctgaag tcacgattcg caaccagcca
     3001 gtgatgaagg ctcacccaac cgatccagat gtgctgtatt ttgtttttgg aacctatttt
     3061 cagggctatg gcaccgacct tttccggttt gatgtcaaat caaagaaact ggataaaacg
     3121 cacaacgact ataatgatat tgattcgatt gaattttccc gaaccgaccc ttcgactatg
     3181 tatttaggac tcgaagttga gcaggttcag taggagcggg ccactgtgtc cgtcaggtct
     3241 tcaattcggg cggacaccgt ggtctgctga taacaagggc ggacacagtg gtccgccccc
     3301 tcccaaaaac catgacctcc attgccttta ttacctgtga tcgaattcca aatttaactg
     3361 ctgatgacca gcttgtggtc gaggaacttc gcaagctcgg tgtgggtata actccagtcg
     3421 tctggacgga taatcctgat ctgtcagggt ttgaactggt tgttgtgcgg tcaccctggg
     3481 actaccatct caaattcgac cagtttcaga actggctcag tgccgtcgag caggatcgca
     3541 tcccgttgct caacccggcc ccaatcatca aatggaatat gaataagcag tacctgcgcg
     3601 agcttgaggc gcgtggggtg ccggttgttc cgtcggtgtg gattcaacgc ggaaatactg
     3661 gcccgttgca ggctgttctt gacgaacaac aatgggatca ggtcgtcgtg aaaccaactg
     3721 tttcggccac ggcttatgaa acgcatcggg tgacaaaaca gcaaaccgga aaattccagg
     3781 cccagt
//
LOCUS       DAOQDO010000313         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17395_length_3785_cov_2.636729, whole genome shotgun sequence.
ACCESSION   DAOQDO010000313 DAOQDO010000000
VERSION     DAOQDO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17395_length_3785_cov_2.636729"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKZ53_06365"
     CDS             complement(<1..453)
                     /locus_tag="PKZ53_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40095.1"
                     /translation="MFANIPLMLSLLAILLVVGGFLFRSTATDAPKFWGFPNTYVLIF
                     SVLVCTAVATWMIPSGKYERAKDKEGREIVVAGSFHRVEQTPQGVDKLLQAPVKGIAQ
                     TIPIITFIFIIGGAFAVLNKTGTIDVSIYWVVERLKGRGELLIPIVMVL"
     gene            complement(457..1326)
                     /locus_tag="PKZ53_06370"
     CDS             complement(457..1326)
                     /locus_tag="PKZ53_06370"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNJ40096.1"
                     /translation="MKSFRLWPVVAIFWFSVCCLMLAASPCVLAGERLVLVGGGKRPV
                     QAVARFVDWAGGPEARIVIIPWASGEPQESYDALAKEINEHHPAAVECAPFAPLTAES
                     KARFLAQLKTAGGVFFTGGDQSRIMDVLADATLLAELRHRYTEGTVFGGTSAGTAIMS
                     RIMLTGDGDFEVIDGGKVVTREGLGLLPNTIVDQHFIKRQRENRLFGLVLQHPEALGI
                     GVDEDNALLVMENRYAEAIGPTQVMMVDGKARRGALTVYLVHSGEKFDLVKRQPVRSF
                     PTSLVKKTAGGAK"
     gene            1734..2609
                     /locus_tag="PKZ53_06375"
     CDS             1734..2609
                     /locus_tag="PKZ53_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HNJ40097.1"
                     /translation="MKPVLIVHGGCGTPPPEEEKLRMDACERAADAGWQVLRQGASAI
                     EAVEVAIRQLEDEPLLNAGTGSYLQADGVARMDASLMASDGRAGSVAQIPGLKNPIRL
                     ARYLLEQDAHVMICGQEALNLALRLGFDPAVVATEAKITYWREHLTEHNMRLDYQKMA
                     DEWKSENPRLGTVGCVALDTEGRLAAGTSTGGTGQCYPGRIGDTPIIGAGTFCTPLVG
                     VSLTGVGERIMVLLSAKRLSDLVKDGKSLEAAGQIVLAEIDELASFAGLIAIDHQGNA
                     IAHKNTPFMSTAIRS"
     gene            complement(2731..>3785)
                     /gene="selA"
                     /locus_tag="PKZ53_06380"
     CDS             complement(2731..>3785)
                     /gene="selA"
                     /locus_tag="PKZ53_06380"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNJ40098.1"
                     /translation="LEYDLGSGKRGQRQVHCEALLQKILACESAVVVNNCAASVLLTL
                     DTFCQDGEVLISRGELIEIGGGFRIPDVMKKSRARLVEVGTTNKTRLSDYEQAISPQT
                     RMILRVHPSNFRLIGFSQKPELAELATLAKHHNLLCFEDLGSGALIDLTPFGIDEPLV
                     KDSIAAGVDLVAFSGDKLLGGPQAGILAGRRDLVSQIKNNPLMRALRVDKMVYAALEA
                     TLRLYLNGLAAEEIPALIALTARPEHLAHRARRFAQRLKRKLNTSKLPVSMTLQPGFS
                     LVGGGSAPQVKLPTTLIGLRSTLFSAVELETRLRSASTPVISRVEHDFVFLDLRTVSP
                     ADEKHLEAVLLSLKPE"
BASE COUNT          921 a         1006 c         1002 g          856 t
ORIGIN      
        1 caacaccatc acaattggaa tcagaagttc gccccggccc ttgagtcgct caacaaccca
       61 ataaatgctg acgtcaatgg ttccagtctt atttaaaacc gcaaaagccc cgccaatgat
      121 aaaaataaag gtaataattg gaatggtttg cgcaattcct ttcactgggg cttgcaggag
      181 cttgtctaca ccctgcggcg tttgttcgac ccgatgaaag gacccggcca ctacgatttc
      241 gcggccttct ttgtctttgg cgcgttcata cttgccactc ggaatcatcc aggtggcgac
      301 tgctgtgcaa accagcactg aaaaaatcag cacgtaggta ttgggaaatc cccaaaattt
      361 gggagcatcc gttgcggttg atcgaaacag gaaccctccc acaaccagga gaattgccag
      421 cagtgaaagc atcaggggga tattggcaaa catgggttac ttcgcgcctc cagcagtctt
      481 tttcaccagc gatgtcggaa aacttctgac tggctggcgt ttgaccagat caaatttctc
      541 ccctgagtgt accagataga ctgtcagcgc cccacgccgg gctttcccat ccaccatcat
      601 cacctgggtt gggccaatgg cttcggcata acggttttcc atcaccagaa gtgcgttgtc
      661 ttcatccacc ccaatcccaa gggcttccgg gtgctgaagc accagcccaa agagccgatt
      721 ctcacgctgc cgtttgataa agtgttgatc cacaatcgtg ttgggcagca gtcccaatcc
      781 ttcacgcgtc acgaccttgc caccgtcaat cacttcgaaa tcaccatcac cggtcaacat
      841 aatgcgcgac ataatggcag ttccggcgct ggtaccgcca aaaaccgtgc cctcggtgta
      901 acggtggcgc agttcggcca gtaatgttgc gtccgcaagc acatccataa ttcggctctg
      961 gtcgccgcca gtaaaaaaca cgccgccagc cgttttcaac tgagccagga atcgggcttt
     1021 tgattcggcg gtgagcggcg caaagggggc acattcaacc gcggctggat ggtgctcatt
     1081 gatttcttta gccagcgcat cataactttc ctgcggctcg ccgcttgccc aggggataat
     1141 cacaatccgg gcctcggggc cgccagccca gtccacaaat cgggccacag cctggacagg
     1201 tcgctttccg ccaccaacca gaaccagccg ttcgccggcc agcacacagg gagacgccgc
     1261 cagcatcagg caacacacag aaaaccagaa aatggcaacc accggccaca accgaaagga
     1321 tttcatggga cacttcccga acagaaagat ggagtgaaag atgagtcttg gaggactaag
     1381 aaaagtctgt atcaatactt gaattaaaca ttacgtttaa gctatattcc gtaatgttta
     1441 ttacaagtat agaaaatttt gtcttactgt caagataaat tattcaggtg ctggcaatct
     1501 ggagcaatca agggtaatcc actgaaatca aagggaaaaa acttgattac cggtggttgg
     1561 cggcgagcat gcgatgaaaa ttcatccaca accaggactc atcctgtttc gggctcgggg
     1621 cttggggcgc cgggttcagg gaaaaataaa ttgttcaatc attcagcctt ttgctcatcc
     1681 agggcggtga tttgaagcgg gtgtcatctt atatatagac gaaggagaat ctcatgaagc
     1741 cagtcctcat tgttcacggt ggctgtggaa cgccacctcc ggaagaagaa aaactgcgga
     1801 tggatgcctg cgaacgagcg gctgatgccg ggtggcaggt gttacgtcag ggcgcgagcg
     1861 ccattgaagc ggttgaggtc gccatccgcc agcttgaaga cgaaccactg ttaaatgctg
     1921 gtacgggtag ctatttacag gctgatggcg tggcccgaat ggatgccagt ttgatggcca
     1981 gcgatggtcg agccgggtca gtggctcaga ttccagggct caaaaacccg attcgccttg
     2041 cgcggtactt attagagcag gatgcccacg tcatgatttg tggccaggag gccctgaatc
     2101 tggcgctgcg tctggggttt gacccggcgg tggtggcgac tgaggccaaa attacctact
     2161 ggcgcgaaca tttgaccgag cacaatatgc ggctcgatta ccagaaaatg gctgacgagt
     2221 ggaaatctga aaacccacgc ctgggcacag tcggctgcgt tgcgctcgac acagaaggcc
     2281 gtctggccgc cggaacatca actggcggta cgggtcagtg ctaccctggt cgaattggcg
     2341 acaccccaat cattggtgct ggaacctttt gcactccgct ggttggggtg tcactgactg
     2401 gggttggcga acggattatg gtcctgctct cggccaagcg gctgagcgac ctggtaaagg
     2461 atggcaaatc acttgaagcc gccggccaga ttgtcctggc tgaaattgat gaactggcca
     2521 gctttgctgg gctaattgcg attgaccatc aggggaatgc cattgcccac aaaaacacgc
     2581 cttttatgtc cacggcgatt cgatcctgaa gaaccgggtt ccgggttccg ggttccgggt
     2641 tccgggttcc gggttccggg ttttcgaaga gggcgcggtt caaagcaaca gcgcccttcc
     2701 tttttgtcat cctgtcacct tgtcaccttg ctattctggc ttgagggaga gcaaaacggc
     2761 ttccagatgt ttttcgtctg ccggtgagac ggttcgcaga tcgagaaaaa caaaatcatg
     2821 ttccacccga ctaattactg gcgttgaagc tgaccggagc cgggtttcca gttcaacggc
     2881 tgaaaacagt gtcgaacgca gaccaatcaa tgtggttgga agcttgacct gtggcgctga
     2941 accaccacca accagagaaa atcctggctg gagcgtcatt gaaaccggaa gctttgatgt
     3001 gttcagcttt cgcttgaggc gttgagcaaa ccgccgggct cggtgggcca gatgttctgg
     3061 cctggcggtt aaagcgataa gcgccgggat ttcttcagcg gctaaaccgt tgagatagag
     3121 tcggagggtt gcttcaaggg cggcataaac cattttatca acccggaggg ctcgcatcag
     3181 ggggttgttt ttgatttgac tgaccagatc acggcgtccg gcaagaattc cagcctgcgg
     3241 tccacccaat agtttgtctc cactgaaagc aaccagatca acgccagccg caatcgagtc
     3301 tttgaccagc ggttcatcaa ttccaaaagg tgtcagatca atcagggcac cactgccaag
     3361 atcctcaaaa cacagcaggt tatggtgttt cgccagggtc gccagttccg ccagttcagg
     3421 tttctggcta aacccgatca agcgaaaatt ggacgggtgg acgcgtaaaa tcatccgcgt
     3481 ttggggtgag atcgcctgtt catagtcaga aagacgggtt ttattggtgg ttccgacttc
     3541 aaccaaccgg gcacggcttt ttttcatcac gtccgggatg cgaaatccac cgccaatctc
     3601 aatgagttct ccgcgagaga ttaaaacctc tccatcttga caaaacgtgt ctaatgtgag
     3661 taatacagac gccgcacagt tattcaccac aacggctgat tcacaggcca gaattttttg
     3721 cagcaacgcc tcacaatgca cctgacgttg ccctcgcttt ccacttccca ggtcatattc
     3781 caggt
//
LOCUS       DAOQDO010000314         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17435_length_3781_cov_3.623725, whole genome shotgun sequence.
ACCESSION   DAOQDO010000314 DAOQDO010000000
VERSION     DAOQDO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17435_length_3781_cov_3.623725"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKZ53_06385"
     CDS             <1..181
                     /locus_tag="PKZ53_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40099.1"
                     /translation="CSPVLVPDETKIKGQKGHQGQGESRFGGRDTETLVNFQATKLSK
                     IPWCQPDPELASTDV"
     gene            296..1039
                     /locus_tag="PKZ53_06390"
     CDS             296..1039
                     /locus_tag="PKZ53_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNJ40100.1"
                     /translation="MSDPLPGKFLTAEWRHLLMLNYEIDPAVLAPYVPKGTEIDFWDG
                     RTFVSLVGFMFLKTRVLGIPIPFHRNFEEVNLRFYVRHKGPEGWRRGVAFIKEIVPRF
                     AIAAVARGVYNENYVSLPMRHTLELEGDCFPATGGTVEYGWQFQGQWHSMKAIASGAP
                     GPLQAGSEAEFITEHYWGYAAQVDGGCVEYRVEHPPWRVWSVREWNLVCDAMALYGPE
                     FGQVLTQPPSSAFLAAGSEITVRKGIRLY"
     gene            complement(1211..1780)
                     /locus_tag="PKZ53_06395"
     CDS             complement(1211..1780)
                     /locus_tag="PKZ53_06395"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNJ40101.1"
                     /translation="MGRFEPRVTNQLPTSKVTRVISAVMILALAALGSGYVTALGQKQ
                     GKNSKNPSQTTNRNTGASANQNRNSSNNLNTNALIPQPAEAFTATAYCLKGRTATGKT
                     TERGMIAADVRVLPLGSVVKIHAGKYSGIYHVADTGSKIRGNKIDVFVPSKSEAVKFG
                     RQKIRVEVLQHGRSVRKPQTGYTTSSPKR"
     gene            complement(2015..3613)
                     /gene="lnt"
                     /locus_tag="PKZ53_06400"
     CDS             complement(2015..3613)
                     /gene="lnt"
                     /locus_tag="PKZ53_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNJ40102.1"
                     /translation="MIELSTIRNWLVTHPLALASGAGVVSACLLVLSFPPFDMWWLAA
                     IAILPLIGAAQVIRQPVPCFFSGWIAGTGVYYGGCWWISHSMIVYGGIHVLAAHGLTL
                     VIAAILGFFTGLFAGGVAVASRLRPWLGLVAAPWMWVAGEYLRIHLADMGWISLGYTL
                     ALHPTLIQLARFTGIYGLSFGLVAFAALGWWALQGRRQAMIATICALVAIGLVWVDGR
                     NTMEATRRLRQTREQVDIVAVQPMVPVLFDSDAEFTQAFQRQVDLSRQGMKHVSSPTR
                     LIVWPESQSSFLLNDSESFELKAIQHLAAEQQAGVLFNSVRRAAGGKFTNIAAVVDPT
                     GAVSSEYTKVHLLPFGEYMPLRSLFEQVGFTPLALDAVPASRPVPTRFGGKTFGTTIC
                     FEAVFPDLDASFRRQGAGALINIANDGWFGKTHGTEQHYRHLVIRAVENQLDTVRVTN
                     TGISVFILATGEVVDRTKQQEATVKVWPLRYGFESERLTFYAAHGDVLAILACLISAL
                     ILLVSIYFGWKLNPSPDTTPRSKT"
BASE COUNT          964 a          956 c          977 g          884 t
ORIGIN      
        1 ttgttcccca gttttggtac ctgatgagac caaaatcaaa ggacaaaaag gacaccaggg
       61 acaaggtgaa agtcggtttg gtgggcgcga cacagagact ttggtgaatt ttcaggccac
      121 taaattgtcc aagattccct ggtgccagcc ggatcccgaa ctggccagca ctgatgtttg
      181 agccaggtgg tgctcttttg ggcaaaattg aggtcattct tcattcttca ttcccaattc
      241 ttcattcttc attctcaatt cttcattccc aattcttcat tcccaattct tcattatgtc
      301 tgatccactt cctggtaaat ttctgacggc tgaatggcgc cacctgctga tgttaaatta
      361 tgaaattgac ccggcagtcc tggcacccta cgttccaaaa ggaacggaaa ttgacttctg
      421 ggatggccgc acctttgtca gcctggttgg cttcatgttt ctgaaaacca gagttctggg
      481 gatccccatt ccatttcacc gcaattttga agaagtaaat ttacggtttt atgtgcgaca
      541 caaaggccct gaaggctggc ggcgtggggt ggccttcatt aaagaaattg tgccgcgctt
      601 tgccatcgcg gcggtcgccc ggggggtgta caacgaaaat tacgtttcgc tgccgatgcg
      661 acacaccctg gaactcgaag gcgactgttt tccagccact ggtggcacgg tcgagtatgg
      721 gtggcaattt caaggccagt ggcactccat gaaagcaatc gccagcggtg ccccaggccc
      781 gttacaggcc ggatcagaag ctgagtttat tactgaacac tattggggtt atgcggccca
      841 ggttgatggc ggctgtgtgg aatatcgggt tgagcacccg ccctggcgag tttggtcggt
      901 tcgtgagtgg aatctggtgt gtgatgcgat ggcgctgtat gggccggaat ttggccaggt
      961 gttgacccaa ccacccagct cggcgtttct ggcggcaggt tcagaaataa cggtacggaa
     1021 aggaatccgg ctgtattgaa gctcaggacg agcaaaagtg gggattggac gtggtaggac
     1081 ttgtaaatga agtcaaaatg aggccatcac aaaaaataaa gtcacagaca aaaacaactt
     1141 tcaatacagc cggacaaaat gaaggaatga caaactttcg cgaaatatcc tgcttacgcg
     1201 gagcgagatg ttatcgtttt ggtgagctgg tggtgtatcc agtttggggc ttacgaactg
     1261 atcgcccgtg ctgcagcacc tcaactctga ttttttgccg cccaaatttt actgcctcag
     1321 atttggatgg cacgaaaaca tctattttat ttcccctgat cttactgcca gtatcggcaa
     1381 cgtggtaaat gccggaatat ttcccggcgt ggatcttcac cacggaccct aatggcagga
     1441 ctcgaacatc cgccgcaatc attccacgtt cagttgtttt tccggttgcc gttcgccctt
     1501 tcaggcaata ggccgtcgcg gtaaaggctt cagccggttg tggaattaag gcattggtgt
     1561 tcaagttatt tgaagaattg cggttttggt tggcagaagc gccagtgttt cggttggtgg
     1621 tttgagacgg atttttgctg ttttttccct gcttttgacc aagtgcggtc acatagccag
     1681 agccaagtgc agccagcgcc aaaatcatca cagccgagat aactcgcgtt acctttgatg
     1741 tgggaagttg gtttgttacc cgtggttcaa atcggcccat tgaaaaacct ccttgtctgc
     1801 cttagccaat atcctatatc ggcatagcat cgtgatcacc ataagcatgc ttcttctacc
     1861 gatatcgtca aaccaggttg aactcaaccg gcatacacca tcattgaaca cggtttgaaa
     1921 gcgtggttca tcatgtgtat tgagaggttt tatgcttaca gcttgagtgg tcgaatagga
     1981 tatatgcgaa agtccagcca ttatagcagg tgtgtcaagt cttgcttctt ggggtggtat
     2041 cgggagaggg gtttaatttc cagccgaagt atattgaaac caaaaggatg agcgctgata
     2101 tcaggcaagc cagaatggcc agaacatcgc catgagcggc ataaaatgtg agtctttcag
     2161 actcaaagcc ataccgaagc ggccagactt ttaccgtcgc ttcttgctgc tttgtccggt
     2221 caacaacttc cccggtggcc aggataaaaa cactgatccc ggtgttggtc acgcgtaccg
     2281 tatcgagttg attttcaacc gctcgaatca ccagatgccg atagtgctgc tctgtaccgt
     2341 gtgtttttcc aaaccagcca tcattggcaa tgttaatcag tgccccagcc ccttgtcggc
     2401 gaaatgaagc gtccaggtcg gggaaaactg cttcgaaaca gattgtggtg ccaaaagttt
     2461 ttcctccgaa tcgcgtcgga acaggacgac tggcaggaac ggcatccaga gcaagcgggg
     2521 taaatccaac ctgctcaaac aggctacgga gtggcatata ttcgccaaag ggcagcaaat
     2581 ggaccttggt atattcactc gacacggcac cagttggatc aaccaccgct gcaatattgg
     2641 taaactttcc gccagcggcg cgtcgaacac tgttaaacag cacacctgcc tgttgctcgg
     2701 cagccagatg ctgaatggct ttcagttcaa aggattcaga atcatttaaa agaaaactcg
     2761 actgggattc tggccacaca atcaaccggg tcggagatga aacgtgcttc attccctgcc
     2821 gggaaagatc aacctggcgt tgaaacgcct gggtaaactc agcatcggag tcaaataaaa
     2881 ctggtaccat tggctggacc gccacaatgt caacctgttc gcgtgtctgc cggagacgac
     2941 gggttgcctc cattgtgttt cgaccatcaa cccacaccag accaatcgcg accagagcac
     3001 agatagttgc gatcatcgcc tgacgtcgcc cctgtaatgc ccaccagcca agcgcagcaa
     3061 aggccaccag cccaaaactc agaccataaa tcccggtaaa tcgggcaagc tgaatcagcg
     3121 tgggatgcaa cgccagtgtg tagccaagtg aaatccaccc catatcggcc agatgaatgc
     3181 gcaggtactc gcccgccacc cacatccagg gtgccgccac cagtcccagc cagggccgga
     3241 gccggctggc aacggcaact ccgccggcaa acaaaccggt gaaaaacccc aggatggctg
     3301 caatgacaag tgtcaatcca tgagccgcca gaacgtggat tccgccatac acaatcatcg
     3361 aatgcgaaat ccaccagcac cctccgtaat aaacccccgt cccggcaatc cacccgctaa
     3421 aaaagcatgg caccggttgg cggatgacct gggcggcccc aattaagggc aagatggcaa
     3481 tggctgccag ccaccacata tcaaacggag gaaaactcaa aaccaaaagg caggcgctca
     3541 caacgcctgc ccctgaagcc agtgccagtg gatgggtaac cagccagttc cgaatggtgg
     3601 acagttcaat cattgttcag ggtttgggat ttggggtttg aggtttagga tttggggttt
     3661 gaggtttggg atttggggtt tggggttcca ggaaaagaca aaaaagacga aaaggacata
     3721 aaggatataa atttcgaaaa cccggaaccc ggaacccgga acccggaacc cggaacccgg
     3781 a
//
LOCUS       DAOQDO010000315         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17459_length_3778_cov_4.334139, whole genome shotgun sequence.
ACCESSION   DAOQDO010000315 DAOQDO010000000
VERSION     DAOQDO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17459_length_3778_cov_4.334139"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKZ53_06405"
     CDS             complement(<1..215)
                     /locus_tag="PKZ53_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40103.1"
                     /translation="MDLGGVFELIRNFVQQDFGVPHFTGFQNDWKPDLGYKPGFQVFT
                     GLKKEKRKTHTPFWLKTAENRAPRTPM"
     gene            235..1668
                     /locus_tag="PKZ53_06410"
     CDS             235..1668
                     /locus_tag="PKZ53_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40104.1"
                     /translation="MKVRPELNLEKWPIWQPANSRTAPRAKVFKREATLSTGDTIVAE
                     VEVGFTQLGMLTTEDQKTYYGLVKYWEDCGRPITEINFSLRRLAEILGKKWGTGTLKT
                     LTDSLARLYATPFFWRNAYYEAPSGEIRMLKSLEGFRILDYLKIVSREENGHVTKELG
                     VFKFNGHILKNLQSHYTKPVFFNVVLNFKSEIAQLLYTHLDLILSDKLVYERRTKELF
                     SDLNITGSSYDNKRSKRKQVLEPALIELFGVPLSKGGVIQQATIEETKDRADFKVVFV
                     KGASDTAGTAVETPNTPDDIEQEQRQVDELLQYFKTIFQTAQSPNSRHRKLATELISQ
                     HGLKLAKSIIDYAHQAAQQTSFRIATFGAISQYVDQAVAELTPSPSQPPAEPDSEEIN
                     HAFEFAYREYWQDQVKRYIAASYTPEEWHILLDSKTNTVLAQNPAFRNWQKDALAEFV
                     EAQVLEDHKPLAQVIAFEEFCLLKQKP"
     gene            1817..2878
                     /locus_tag="PKZ53_06415"
     CDS             1817..2878
                     /locus_tag="PKZ53_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ40105.1"
                     /translation="MASSESFVSVQEAAKILNCGISTVRKMCQEEKIQGVQKVGNGWL
                     IPRTSLNQSPVPNQTRGWPLGKTRLSGEPELPLPAVVSEQVSLAHKIAIFNQAGGVGK
                     TTIVRDLGYELASRGYKTLLVDADPQATLSTFFDFNPAERPEADLFWSPIYRGTKGVK
                     PTVFPTQYANLYLGLANRELKRGELELLEAKDQGRLRGFLKLIHSEFEFILFDCSPAI
                     SEITIQVLFAVDSLVIPVQTQAKSVVAFGEIQYEIGKAQERRMNMDLSPLGVLGIIPT
                     MFRQATMIQKHHFEEMKSLCELFDYALLPPIHDSIKVSEAGSFRKPLKNYDKKCRIND
                     DIEQICSSVLTLLSRPLEK"
     gene            2910..3749
                     /locus_tag="PKZ53_06420"
     CDS             2910..3749
                     /locus_tag="PKZ53_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ40106.1"
                     /translation="MPKKAIPPSFTPHRQSALASFVHEKKSKRIGEIRLALIQPNPFQ
                     PRKNYPDAEIEELTESINQQGLLQNLVCRVEPDGKYTLIAGHRRLLALHKLGKETVPV
                     SILENISDQDLQILALIENLQRKNLHPVDEVRAIVQVIEQAGSQEQAAKLLHKPVGTI
                     GGIAALHNLGSEILEICLGVPDLTKNKLAQLLKLPAERRLAAAMALHSGTPSKIPATK
                     PKSVSPNVFRFRAKTTTDASFSFKLQFKKGNPTEAEILDALNVILQKLKSEVFAPVPT
                     ENS"
BASE COUNT         1055 a          938 c          861 g          924 t
ORIGIN      
        1 cacatggggg ttcggggggc acgattttcc gcagttttga gccaaaacgg cgtgtgtgtt
       61 tttctttttt cttttttcaa accagtgaaa acctgaaaac caggtttgta acccagatct
      121 ggtttccagt cgttctgaaa accagtaaag tggggcacgc caaaatcctg ctggacaaag
      181 tttcttatga gttcgaacac gccacccaaa tccaaaaaac ctcaccaggc tctggtgaaa
      241 gttcgaccag agctcaatct cgaaaaatgg ccgatctggc aaccagccaa ctcgcgaacg
      301 gccccacggg caaaggtctt caagcgcgaa gccaccctct caactggcga tacgattgtg
      361 gctgaagttg aagtcgggtt tacccagttg ggaatgctca ccaccgagga ccagaaaacc
      421 tactatgggc tggtcaagta ctgggaggat tgtgggcgac caatcactga gatcaacttt
      481 tcactcaggc gactggctga aatcctcggg aaaaagtggg gcaccggcac actcaaaacc
      541 ctgacggatt cattagcccg gctctatgcc accccgttct tctggcggaa tgcgtattat
      601 gaagctccga gcggtgaaat ccggatgctt aaatcattag aaggttttcg gattttagat
      661 tacctcaaga ttgtatcccg cgaagaaaac ggacatgtga ccaaggaact cggggtgttt
      721 aaattcaatg gtcatattct gaagaacctg caatcccact ataccaaacc agttttcttt
      781 aatgttgtct tgaacttcaa gtcagagatc gcccaactgc tctatacgca tcttgatttg
      841 attttgagcg ataaactggt gtatgaacga cgcaccaaag aactcttttc agacttgaac
      901 atcactggat cttcatacga taataaacgc agcaaacgca agcaggtgtt agagccagcc
      961 ttgatcgaac tttttggtgt tccgttatca aaaggagggg ttatccagca ggccaccatc
     1021 gaagagacca aagaccgggc tgacttcaaa gttgttttcg tcaaaggggc atccgacacg
     1081 gctggcaccg cggttgaaac gccaaacacc cctgatgaca ttgagcagga acaacggcag
     1141 gtggacgaac tccttcaata ctttaaaacc atttttcaaa cagcacagtc ccctaattct
     1201 cgccaccgaa aactggcaac ggagctgatt tcacaacacg gtctgaaact ggccaaatcc
     1261 attattgact atgcccatca ggccgcccag caaaccagtt ttcggattgc cacctttggg
     1321 gccatcagcc agtatgttga tcaggccgtg gccgaattaa ccccttcccc gtctcaacca
     1381 ccagcggaac cagactctga ggaaatcaac catgcctttg agtttgccta ccgggaatac
     1441 tggcaagatc aggtcaaacg atacattgcc gccagttata cccctgaaga atggcacatc
     1501 ctgctggatt caaaaaccaa cacggtgctg gcacaaaatc cggcttttcg aaactggcaa
     1561 aaagacgcac tggccgaatt tgttgaggca caagtgctcg aagaccacaa accactggct
     1621 caagtcattg cctttgaaga gttctgcctg ttgaagcaaa aaccgtgatg cggttcctgt
     1681 cgctttcctg atctgaccgt ggtcgtcgca caacaaattc cttgccttac atagcgatat
     1741 cgctatatat agatacttct ttttcagatt tgattggtac atcacacttt tataaagaga
     1801 tgaaggaccg tcgcatatgg cgtcatcaga aagctttgtt tctgttcagg aagcggcaaa
     1861 gattttgaat tgtgggattt cgactgtcag gaaaatgtgc caggaagaga aaattcaagg
     1921 ggtgcagaaa gttggaaacg gctggttgat tcctcgaact tctctcaatc agtctccggt
     1981 tccaaatcag actcggggct ggccgctcgg aaaaactcgc ctgagtggtg aaccggaact
     2041 cccgttaccg gcagttgttt ccgagcaggt ttcccttgcc cataaaattg ccatttttaa
     2101 tcaggccggc ggggttggga aaaccacgat tgtgagagac ctgggctatg aactggcttc
     2161 acgaggttac aaaacactgc tggttgatgc cgatccacag gccacgctga gcactttttt
     2221 tgattttaac ccagccgaac gacccgaggc tgatttattc tggtctccca tttatcgcgg
     2281 tacaaaaggt gtgaagccaa cggtttttcc aactcagtat gcaaatttat atctggggct
     2341 ggccaatcgc gaattgaaac gcggagaact cgaactcctt gaagccaaag atcaaggccg
     2401 cttacgtggc tttctcaaac tgatccattc cgagtttgag ttcattctat ttgattgttc
     2461 cccagcgatt tctgaaatta caattcaagt gttatttgcc gttgattcac tggtgatccc
     2521 ggttcaaacc caggcgaaat cagtcgtggc gtttggtgaa atccaatatg aaattggaaa
     2581 agcccaggag cgccgcatga atatggattt atctccgctt ggggtgcttg ggattattcc
     2641 caccatgttt cgtcaggcaa cgatgatcca aaaacaccat tttgaggaaa tgaaaagcct
     2701 gtgtgagttg tttgactatg ccctgctgcc accaattcat gactcaatca aagtttctga
     2761 ggctggatca tttcgcaagc cgctcaaaaa ctatgataaa aaatgccgga tcaacgacga
     2821 tatcgagcaa atctgcagtt cagtcctgac actgctttcc aggccacttg aaaaataagg
     2881 aaattttcag ccactccggc tcaactgtta tgcccaaaaa agcgattccg cccagtttta
     2941 cgccacaccg tcaatctgca ctggccagct ttgttcatga gaaaaaatca aagcgaatcg
     3001 gtgaaattcg ccttgctctg attcaaccca acccatttca accacgaaag aattatccag
     3061 acgctgaaat tgaggagctg accgaaagca tcaaccagca aggattgctg caaaacctcg
     3121 tctgccgggt tgagccggat ggcaaatata cattgattgc cggccatcgc cgattgctgg
     3181 cactgcacaa acttggaaaa gagacggttc cggtcagtat tctggaaaat atctctgatc
     3241 aggatttgca aatcctggcg ttgattgaaa atctgcaacg gaaaaacctg catcccgttg
     3301 acgaagtacg ggctattgtc caggtgattg agcaggcggg gtctcaggaa caggcggcaa
     3361 aactccttca caagccggtt gggacgattg gcggaattgc cgcactccac aatttaggca
     3421 gtgaaatcct tgaaatctgc cttggtgtgc ctgatctgac aaaaaataag ctcgcccaat
     3481 tgttaaaact tccggcggaa agacgactgg ccgcagcaat ggcgcttcat tctggaacgc
     3541 cttcaaaaat tccagccacc aaaccgaaat cagtttcacc aaacgttttc cggtttcgag
     3601 caaagacgac aaccgacgcg agcttttcct ttaaattaca gtttaaaaaa ggaaatccaa
     3661 ccgaagctga aatcttggat gccctgaatg tcattctcca aaaactcaaa tcagaggtgt
     3721 tcgcaccagt tccaactgaa aattcttgaa ggttcaagaa ttgctgcaat ccactctc
//
LOCUS       DAOQDO010000316         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17479_length_3776_cov_3.914270, whole genome shotgun sequence.
ACCESSION   DAOQDO010000316 DAOQDO010000000
VERSION     DAOQDO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17479_length_3776_cov_3.914270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3047
                     /locus_tag="PKZ53_06425"
     CDS             <1..3047
                     /locus_tag="PKZ53_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40107.1"
                     /translation="ANLAVCALAVVPGPTLDQFVIYAGTGEGFLSLDVTRGGSGASGN
                     GIFRSTNGGATWQHLPDTINNSNFEYVNDLVVSPTNPNRVYAATQTGIWRTINGGDSW
                     QQVYATDVRGGAMDLALRTDQPTDFLFATIGTFAQAAILRNKDAAGTGNWETVLNETG
                     MGRTSLAIAPSDQSTIYAMSASIESRFDFNYTNGLHAVFRSTSNGDPGSWSARVRNTD
                     PVRLNTVLLSNTIFAFLNECRIFSGPTTFFNQGWWDNIIAVDPINPDIVFAGGIDLFR
                     SDDGGANWGMISHWWASPEAPRYSHADHHDIVFHPQYDGARNQTMFVTSDGGIERTDN
                     ARGPSATGDQAPCSTENGGVFWRSLNNGYSVTQFYHGTAYQSARFYFGGTQDNGTVRG
                     GDSTGINWGRVFGGDGGYVAIDPVNLLTQYVSTPNGSFYVTNDGGRTFEIGSFGLADR
                     RFLFITPFAMDPKNPKRFWTGGQRMWQCDNAMVFWDFGSAPLYEGESFSSVSAIAISP
                     QDSNIVLAGRADGFIHRTTAALSSNRETVWPAVQPREGFVSSLAFDPKNPSVVYATYS
                     TFGGNHVWQSTDAGASWMPFDGVGSGKLPDLPVHSVLVDPGNSARVFVGTDLGVFTTL
                     DSGRTWLVENTGFANTVVQTLCQDNGNLVAFTHGRGAWKVSLASQPDPTPAIAGFFPT
                     SGPVGTTVSVSGSNLTGATQVRFGDVPATSFSVASANQLTAVVPSGTPGGQLRVTTPN
                     GTAVSAGSFTVTFAPPTITSFSPTSGESGTVVTITGTQLTGATNVQFGQASAPFEVLS
                     STQLQATVPIGADSGPIVVTTANGTATSQESFTVVVKIPKIVGFTPQSGPVGTQVTIN
                     GLNLSNTGGVSFGNVAASVFQVLSDTQLSATVPTGATTGVISVITPTGGASSDRAFIV
                     EGPDFSLAATASNPTPARGGKITVTVTIARTGGLTDQITVTAPTLAGFKFKPASQTTS
                     GTSVSFTVKIKNNAPQGQQTLVFSGRDASGRTRTVELVITIR"
     gene            3370..>3776
                     /locus_tag="PKZ53_06430"
     CDS             3370..>3776
                     /locus_tag="PKZ53_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40108.1"
                     /translation="MFRLLSVLFLVLVLMIPAQVAASFGGQREKQLKPERQKETGDQP
                     SLSDVGRHQRVKERKADRTTRDDGDENTRENADDGQNDKRPTPGRLEEPDQLPALTDA
                     AFRQGFVDGKPGGDQEARERGRDLGRASGHQRG"
BASE COUNT          854 a         1029 c         1021 g          872 t
ORIGIN      
        1 tggccaatct cgcagtgtgt gcgctggcgg tggtgcccgg tccaaccctt gaccagtttg
       61 tgatctatgc cgggacgggc gaaggatttc tgtcacttga cgttacccgt ggcgggagtg
      121 gtgcatcggg caatggaatt tttcgctcta ccaatggcgg cgccacctgg caacatctgc
      181 ccgacacaat caacaactca aacttcgagt atgtcaatga tctggtggtc agcccgacca
      241 atcccaatcg cgtctatgct gccacccaaa ccggcatctg gcggacaatc aatggcggcg
      301 actcgtggca gcaggtgtat gccaccgacg tgcgcggtgg ggcaatggat ctggcgcttc
      361 gaactgacca gccgactgat tttctgtttg ccacgattgg cacgtttgcc caggcggcaa
      421 tcttgcgcaa caaagatgcg gcaggcactg gaaactggga aacggtgctc aatgaaaccg
      481 ggatgggccg aacctcgctg gccattgcgc cttcggatca aagcaccatc tatgccatgt
      541 cggccagtat cgaaagccgg tttgacttta attacaccaa tgggttacac gcggtgtttc
      601 gatcaacttc aaacggcgac cctggttcct ggtcagcccg cgtgcgcaat acagatccag
      661 tcagactcaa cacggtgctg ctctcaaaca ccatttttgc ttttttaaac gagtgccgga
      721 tttttagcgg ccccacgacc tttttcaatc agggctggtg ggacaatatc atcgcggttg
      781 acccgatcaa cccggacatt gtgtttgccg gcggcattga cctgtttcga tcagacgatg
      841 gcggcgccaa ctggggcatg atcagtcact ggtgggcttc acccgaagca ccgcgctatt
      901 cgcacgccga ccatcacgac attgtgtttc atccacaata tgacggggcg cgcaatcaaa
      961 ccatgtttgt gaccagtgat ggggggattg aacgaaccga caatgcccgt ggcccatccg
     1021 ctacaggaga tcaggccccc tgtagcactg aaaatggcgg tgtgttttgg cgttcgctca
     1081 acaatggcta ttccgtcacc cagttttacc atggaacagc ctatcaaagt gcccgctttt
     1141 actttggcgg cacccaggac aacgggacgg ttcgtggcgg cgattcaacc ggaatcaact
     1201 gggggcgtgt gtttggcggc gatggcggct atgtggcgat tgatccagtc aatttgctga
     1261 cacagtatgt ttcgacacca aacggcagct tttatgtcac aaatgacggt ggacgaacct
     1321 ttgaaatcgg atcatttgga ctggctgacc gcagattttt atttatcact ccgtttgcca
     1381 tggatccgaa aaatccaaag cggttttgga ccggcggtca gcgcatgtgg caatgcgaca
     1441 acgccatggt tttctgggat tttggcagcg ccccgctcta tgaaggagag agtttttcga
     1501 gcgtgagcgc gattgcgatt tctcctcagg actcaaacat tgtcctggct ggtcgtgccg
     1561 atggatttat ccaccgaacc accgctgcgc tgagttccaa tcgcgaaacg gtgtggccgg
     1621 cggttcagcc ccgcgaaggg tttgtgtcgt cactggcttt tgatccgaaa aacccatctg
     1681 tggtctatgc gacctattca acgtttggtg gaaaccacgt ctggcaaagc accgatgccg
     1741 gggcaagctg gatgccgttt gatggcgttg gaagcggaaa attaccggat cttccggtgc
     1801 attcggtact ggttgaccca ggaaatagcg ccagagtgtt tgtcggcact gatcttgggg
     1861 tgtttacaac gctggatagt ggtcgcacct ggctggttga aaacaccgga tttgccaaca
     1921 ccgtagtcca aactctgtgt caggacaatg gaaacctggt cgcctttacc cacggacgtg
     1981 gtgcctggaa ggtttcactg gcctcccaac ctgacccaac accagccatt gctggctttt
     2041 tcccaacttc cggcccggtt ggaaccacgg tcagcgtgag tggtagcaat ttgactggcg
     2101 ccactcaggt tcggtttggc gatgtccctg ccaccagttt ttcggtcgct tcagccaatc
     2161 aactgacggc ggtggtacct tctggaactc cgggcggtca gctacgtgta accacgccga
     2221 acggaacagc cgtgagtgcc ggatcattca ccgtgacgtt cgcacctccg acgattacca
     2281 gtttcagccc aaccagcgga gagtcaggca ccgtggtcac cattactggc acccagttaa
     2341 ctggagccac gaatgttcag tttggccagg catctgcccc atttgaagtt ttgtcgagca
     2401 cccagcttca ggcgacggtt ccgattggcg ctgattcagg accaattgtg gtgacaacgg
     2461 ccaatggcac ggcaaccagt caggagtcat ttacggtggt tgtcaaaatc ccgaaaattg
     2521 tcggattcac acctcaaagt gggcccgtcg gtactcaagt cacaatcaat ggcctgaatc
     2581 tgtcaaatac tggtggtgtc agttttggaa atgttgcggc aagcgtcttt caggttcttt
     2641 cagataccca actcagcgcc acggttccaa ctggagcaac cactggtgtg atctcagtca
     2701 ttacgccaac cggaggcgcc tcgtctgatc gtgcatttat tgtcgaagga ccggacttct
     2761 cactggcggc aaccgcgtcc aatccaaccc cggcccgggg tgggaaaatc acggtgacgg
     2821 tcaccattgc ccggaccggg gggttgactg atcagattac agtgacagca cccaccctcg
     2881 ccgggttcaa attcaaacca gcttctcaaa ccacaagcgg caccagcgtg agctttacag
     2941 tcaaaatcaa gaacaatgcc ccgcaaggcc agcaaacact tgtttttagt ggtcgtgatg
     3001 ccagcggtcg aactcgcacg gttgaactgg tcatcacgat tcgataactc aatgaaatct
     3061 caggcaagga agcaggaaaa gcagtccctc agtcaacctg tttccttgca caaccagcca
     3121 actcagtcca tattttagaa aaccagccgg atctcccccg gccagcctgt tctttaccga
     3181 tgacgtcggt ttttcagtcc aagctacggc ttgctcaagg tgtcagccag aaccgatgac
     3241 cgaatgaaaa aagagtggtt gaatgggagc catagctcaa tgttccatct ctcattcttc
     3301 atttggttga gttctttatt tcctttagaa atcagggctt atcttcatat gccacaatat
     3361 gtactcacct tgttccgtct acttagtgtg ctatttctgg tactggtttt gatgattccg
     3421 gcgcaggttg cggcctcgtt tggtgggcaa agagaaaaac agctaaaacc tgaacgccag
     3481 aaagaaaccg gcgaccagcc ttccctgtcc gatgttggac gccatcagcg ggtgaaggag
     3541 cgaaaggcag atcgcaccac gcgtgatgat ggcgacgaaa ataccaggga aaacgccgat
     3601 gatggtcaaa acgataaacg cccaacccct gggcgactcg aagaaccaga ccagctccca
     3661 gctctgactg atgccgcttt tcgtcaggga tttgtggatg gcaaacccgg tggcgatcag
     3721 gaggcccgtg agcgaggccg tgatttgggg cgggcatcag gccaccagcg tgggtt
//
LOCUS       DAOQDO010000317         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17643_length_3760_cov_2.549258, whole genome shotgun sequence.
ACCESSION   DAOQDO010000317 DAOQDO010000000
VERSION     DAOQDO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17643_length_3760_cov_2.549258"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..>3760
                     /locus_tag="PKZ53_06435"
     CDS             363..>3760
                     /locus_tag="PKZ53_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40109.1"
                     /translation="MFSTSMPNPTFWNRLSFYRQIVFCVSGLVGALCLAGVPVLADSA
                     PIFQNAAAKSQPETVPLPKVPTEFKLRPNQVHRYSVDLEGGEFLKIDFEQKGMDLYLI
                     VENGHGKKLFHCNRLESDRGIEPIYFSSETPIRYFVIVGCIYQEDIPGSYEVRPTVHR
                     PPTPQDQHLLRAQELNYEAETIGRAAKDKADFQKSLDTYLEAAREFHAAGDLASEAIM
                     TVFAGRIERTFGNLPKSIEYCTNALKLLETQPDLVIQAAALTNIGRSYRLMEQFETAL
                     DYYEQALVLRRKTDDLVGESIELNNIGSVYVASWEYRKAEEYLKRSLEISQRVGNVSG
                     LASNLGNLATINASLGDFEKTLLYTMQSYQLLVKLNDQNREISILAQIGNLYLKLGKI
                     DEATEYFQKGLDLSVRIGNRQQQIIILHDLARLPNISSATSLQYLNQSLVLARTLKSQ
                     IDEGAALALLGQFHLDQNNIQEAFSYLTQANQIFEATRNQSQIGRIWRFFGKAYVAEK
                     NYPLALEQFQKSLASSEQRGDRTATVNLLNDLALTHLQLGQYNEARADLERAIQLVEQ
                     IRSTVLDQSFRLSYFSSTQSLFENYIYLLMELHRRNPSAGYDREAFQKNEARRARSLL
                     ELLLLPGEGLPDEGSTGLLSQEQNLIERILAREQNLTKTLAESARRERVTRIQEEISA
                     LYNDYRKTQIEIRKQSPHYASLTQPQPLSVEQVQQTCLDDNTLLLEYSLGTPHSYLWV
                     ISKTDCQVFQLAGKAEIEAKTRALYECLKLNPLKRIFFNQETSDKPDTPPANCTEVEL
                     TQLAREVSDLVVGPVAKKLGTKRLLVVADGALHYLPFGVLSTPGQSSYVPLVVEHEIV
                     SIPSATTLALIRQEHTARKPADGTLALFADPVFEPSDPRLKVAGVRGPETEGQKSPPP
                     HPDITETVKQLASGQRNLTAIVSDYNTRLQLTRLPFTRTEATNILRMIPRTDKVLTRF
                     DFEANRDALFSEELSRFRYVHLATHGYFDPKKPEQSALILSLVGPDGKTQDGFVRTSD
                     IYRLNLHADLVVLSACETGLGEIPAVHAGPAVVQFRVVVVVTLPVNPEVKTGIETSSV
                     AQSGVILQKSAGRSGSIQPLGLPGAPGNDVDDAVHGVGTPQRAAR"
BASE COUNT          952 a         1011 c          894 g          903 t
ORIGIN      
        1 gttcagggtt cagggaaata caattttttc aataatttag ctttcccgta atccgatacc
       61 ttcattgcaa aatggtatta acctgatgaa gaacggattc tgctcttgaa agtgcccgcc
      121 cgctcacgta atgatcgaaa tctggtattt cagtttcagt cacgatgtac aacccaggca
      181 tcacaaactc ccctcaaagc attttgcatc cactgctttt cagaaaataa tttctgttta
      241 gtattctcac tttagcttgc agggattttt tcctcgcatc atccaggtga ctggtctcac
      301 gcagtttggt ccaccatcgg attgaaggca agttcaaact taaactcttt accttttcat
      361 ttgtgttttc gacatctatg cctaacccga ctttttggaa tcgcctctct ttttatcgtc
      421 agattgtctt ttgcgtgagc ggcctggttg gcgcgctatg cctggctggg gttccagttt
      481 tggccgattc agcacccatt tttcaaaatg cagccgccaa aagtcaacct gaaaccgttc
      541 cactcccgaa agttccaact gaattcaagc ttcgccccaa tcaggtacac cgatattcgg
      601 tggatctgga gggcggtgaa ttcctcaaaa ttgattttga gcagaagggc atggatctgt
      661 acctcatcgt tgagaatggt catggaaaaa aactgtttca ctgtaaccgg ttggaaagtg
      721 atcgcggtat tgaacccatt tatttctcca gcgagacacc catccggtac tttgtgatcg
      781 tgggctgtat ttaccaggaa gatattcctg gcagctatga ggtgcgacca accgtccatc
      841 gccccccgac accacaggat caacacctgc tccgcgccca ggaactcaac tatgaggcag
      901 aaaccattgg ccgggccgcc aaggataaag ccgacttcca aaaatcactt gatacctatc
      961 ttgaagccgc ccgggaattc cacgcagccg gcgacctggc cagcgaagcc atcatgaccg
     1021 tttttgccgg gcggatcgag cggacgttcg ggaacctgcc aaagtcaatc gaatactgca
     1081 ccaacgcgtt gaagttactg gaaacccaac cggacctggt catccaggca gcggcgctga
     1141 ccaacattgg ccggtcctac cgattgatgg aacagtttga aacggcactt gactattatg
     1201 agcaggcact ggtgctccga aggaaaactg acgatttggt gggcgagtcc attgaactca
     1261 acaacatcgg ctcagtttat gtggcctcat gggaatatcg taaagctgaa gaatatctga
     1321 aacgctcgct tgaaatctcc cagcgagtcg gaaacgtgag cggactggcc tcaaaccttg
     1381 gaaatctggc aaccatcaac gccagtttgg gagattttga aaaaacactg ctctacacca
     1441 tgcagtcgta ccagttgctg gtgaaactca atgaccagaa tcgcgaaatt tcgattttgg
     1501 cccaaattgg gaacctgtat ctgaaactcg gcaaaataga tgaggccacc gagtatttcc
     1561 aaaaaggatt ggacctgtcc gtacgaattg ggaaccgaca acagcaaatc atcatcctcc
     1621 acgatcttgc gcgtctgcca aatatctcat cagccaccag tttacagtat ctcaatcaat
     1681 cacttgtgtt ggctcggact ttgaaaagcc agattgacga gggggcagcc ctggcgctcc
     1741 tgggacagtt tcaccttgat cagaacaata ttcaggaagc gttttcatat ttgactcagg
     1801 ccaatcagat ttttgaggcg acccgcaatc agagccagat cggtcgaatc tggcgtttct
     1861 ttggtaaggc ctacgttgca gaaaaaaact atccactcgc tctcgaacaa tttcaaaaat
     1921 cactggcctc ctcagaacaa cggggtgatc gcaccgcgac cgtcaacctt ctcaatgatc
     1981 tggcactgac gcatcttcag cttggtcaat ataatgaagc acgggctgat ctggaacgag
     2041 ctatccaact ggtggaacaa atccggtcca ccgtgctcga ccaatcgttt cgattgtctt
     2101 acttctcatc aactcaatcc ttgtttgaaa attacattta tttgttgatg gaactccatc
     2161 gtcgcaatcc ttcagccggc tatgaccgcg aagcgtttca aaagaacgaa gctcgccggg
     2221 cgcgcagcct gcttgaattg ctcttgctgc ctggtgaggg tctgccagat gaaggatcca
     2281 ccggtctact ttctcaagaa caaaatttaa ttgagcggat tctggccaga gaacaaaact
     2341 tgaccaaaac cctggctgaa tcggcccgcc gggaacgggt cacccgaatt caagaggaaa
     2401 tctcagccct ctataatgac tatcgaaaga cgcagatcga gattcgcaaa caaagccccc
     2461 actatgccag cctgacccag cctcaaccgc tcagtgttga gcaagttcag cagacgtgtt
     2521 tagatgacaa cacgcttctg cttgaatatt cactgggcac acctcacagc tacctgtggg
     2581 tcatttcgaa aactgactgt caggtctttc agcttgcagg gaaagcagaa atcgaagcca
     2641 aaacccgagc gttgtatgaa tgtctgaaac tcaatccgct caaacgaatt ttcttcaatc
     2701 aggaaacttc ggacaaaccc gatacgccac cagccaattg cactgaagtg gaactgacgc
     2761 aactggcgcg tgaggtctcg gatctggtgg tgggtccggt ggccaaaaag ctcggtacca
     2821 aacgcttgct ggtggtggcg gatggtgcct tgcactacct tccatttggg gtgctgtcaa
     2881 ctccgggcca gtccagttat gtgccgctgg tggtggagca tgaaatcgtt tcgatcccat
     2941 cggcgaccac cctggctttg attcggcagg aacacacggc ccgcaagcca gccgatggca
     3001 cactggcctt gttcgctgac ccggtgtttg aaccctctga cccacggctc aaggtggcgg
     3061 gagttcgtgg gccagaaact gaagggcaga aatctccccc tccccatcca gacattacag
     3121 aaacagtgaa acaactggct tctggtcagc gcaaccttac ggccatcgtg tctgactaca
     3181 acactcgatt gcaactcacg cgactgccgt ttacccgcac cgaagccacc aatattctgc
     3241 ggatgattcc ccgcacggat aaagtactga cccggtttga ctttgaagcc aatcgagatg
     3301 ctttgttttc agaggaactc agccgatttc ggtatgttca tctggcgact cacggctatt
     3361 ttgatcccaa aaagcccgaa caatcagctt taattttatc gctggttggc ccagatggaa
     3421 aaacacagga tgggtttgta cggacctctg acatttacag gctcaattta cacgctgacc
     3481 tggttgtttt aagcgcctgt gaaaccggcc tgggtgaaat tccagcggtc catgccggac
     3541 ctgccgtcgt ccagttccgt gtcgttgtag tcgtaacgct gcccgtaaac ccggaagtaa
     3601 agaccggaat cgagacgtcc tccgtagcgc agtccggcgt aatcctgcag aaaagtgccg
     3661 gcagaagcgg tagcatacag cccctgggtc tcccgggcgc tccgggtaat gacgttgatg
     3721 acgccgttca cggcgttggg accccacagc gtgccgcccg
//
LOCUS       DAOQDO010000318         3748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17735_length_3748_cov_2.434877, whole genome shotgun sequence.
ACCESSION   DAOQDO010000318 DAOQDO010000000
VERSION     DAOQDO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17735_length_3748_cov_2.434877"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..2252
                     /locus_tag="PKZ53_06440"
     CDS             213..2252
                     /locus_tag="PKZ53_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNJ40110.1"
                     /translation="MLPPPETLPIEIIDTLHGQTIVDPYRWLENGEATAVHDWTAQQN
                     TYCRSVLDAFPYREYLRQQVTNCAQLGAISTPLVYGGKYFWTKRSGLQNQAVLYVRET
                     LSGPDRVLVDPNTLSEHGTVTLDWWFPSPDGTRVAIGLSDNGSELSTLSILDVTTGEY
                     LPDIIPDTRFVSLAWMPDGHGFYYTRYPGRGTRPTGQEHYHRWIYFHRLGSHFEADPV
                     VFGPHRKPEEYFHVQVSPSGRYVLVTASRTFDYQDLYLLDVQSDLGFVPIVENINASF
                     FGEFAGESVLIRTNWNAPRFCLMKVNLAELKAPWAMVIRQSASVLESFAVAHDHFVVC
                     SYLTRAQSELRLFDLESSFTCSIPVPKYSTITGLSARWDSTKIFLSLTSFAVPTTVYC
                     FETATQSLTLFDQIESLVDPASYIIKQVIYPSRDQTRVSMFIVHRKHLKFDGMAPTIL
                     TGYGGFNISRTPEFLGSLLTLLDAGAVYALPNVRGGGEYGEEWHKAGMRDRKQNGFDD
                     FLCAAEYLIQHQYTCPEKLAIQGGSNGGLLVGAALTQRPDLFRAVVCQVPLLDMLRYH
                     HFLIARTWIPEYGCAEDQDAFHWLMAYSPYHHVTPETPYPAVLLMTAQSDTRVDPLHA
                     RKMAARLQAATSSGYPILLRVESQAGHGVGKPTAKRIEESVDVLSFIAAQLGISL"
     gene            2265..>3748
                     /locus_tag="PKZ53_06445"
     CDS             2265..>3748
                     /locus_tag="PKZ53_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNJ40111.1"
                     /translation="MIWYLKASQDKPGHARRFRVSWLGLSLLLVLTLSLATGMTSAYQ
                     GGGGYTLQVGSTNDEAAARAQVAQLKAAGHTAYYVRAEIPGKGTWYRIRVGRFGSPAD
                     AQRYSRTLPGKSTFATSYDGPNGSPNSRVPSSEPSAVASKTPTPSTPSKPKSDPAKIA
                     ATTSSKPDTSKPSTAKATPKVDPGKPAAGTTLGKAQSTSVNKPTSQPKTEIANNTPPR
                     PKTVETKSSVRVQPKNTQPALKLETPTVALASKISRRPQLLALPKEPAKSAPAPTTTQ
                     SSTLTASRLDPKKGTSKPQELNSKTPSDTSARPWIAEVSRKEKGEEVASLTLPNLGNP
                     LPPESGVSGGVGLKLVEASTSKALLEAAPAKPPAPKLVKPHPPIKFLTSLSPTPASFK
                     AKDLGALLAAPETPPTGWVRQYPTLRTDLTRVFFADAGHGWAAGIQGMVLSTEDGGET
                     WNSQSIGARGVVNDLFFIDRLRGWALIGGTLGTQARDLRDEQVL"
BASE COUNT          875 a         1066 c          961 g          846 t
ORIGIN      
        1 gccgattcca gcgccctccg ggcgcaaaaa ctgggtggcg ctcaaatccg gtggtatgcc
       61 caaagcggca aaccaccggc tactttccag cgccctccgg gcgggaaaca gaacaaactc
      121 ctttcttatg agaacttgta tttagcccga gttcttcagc cctcagccct cagccctaag
      181 cccgctttct tcagcctaat gtcttccgcc ctatgcttcc gccacccgaa acactgccga
      241 tagaaatcat cgacacgctg cacggacaga ccattgttga tccttaccgc tggcttgaaa
      301 acggtgaggc aacggcggtt cacgactgga cggcgcagca aaacacttac tgccggtcgg
      361 tactggatgc ttttccatat cgcgaatacc tgcgccagca agtcaccaat tgcgcacaac
      421 tgggcgcaat ttcgacaccc ttggtgtatg ggggcaaata cttttggact aaacggtcgg
      481 ggttgcaaaa tcaggcggtg ctctatgttc gtgagacact ttccgggcca gatcgggtcc
      541 tggttgatcc aaataccttg agtgaacacg ggacggtgac tctggactgg tggtttccat
      601 cgccggatgg tacgcgagtc gccattgggc tctcagacaa tggaagcgag ctgagcacgc
      661 tgtcaatttt ggatgtcacc actggcgagt acctgcccga catcattccg gatactcgat
      721 ttgtctcgct ggcctggatg cctgacggtc acggatttta ctacacaaga tatcctggtc
      781 gtgggacacg acccactgga caggaacact atcaccggtg gatctacttt catcgcctgg
      841 gatctcattt tgaagcagat ccggttgttt ttggccctca tcgcaaaccg gaagaatatt
      901 ttcatgtgca ggtctcccct tcgggccgct atgtgctggt gaccgcgtct cgaacctttg
      961 attaccagga tctgtacctg cttgatgtac agagcgattt agggtttgtg ccgattgttg
     1021 aaaacatcaa tgcttctttt tttggtgaat tcgccggtga atcagtgctg attcgcacca
     1081 actggaacgc gccccggttt tgcctgatga aggtcaacct ggctgagttg aaagccccct
     1141 gggccatggt catccgccag tcagcttcgg tgctggaatc ttttgccgtg gctcacgacc
     1201 actttgtggt gtgctcatac ctgaccaggg ctcagtcaga gttgcgcctg tttgatttgg
     1261 aaagctcgtt cacctgttca attccagttc ccaaatacag caccatcaca ggtctgtctg
     1321 ccagatggga ttcaaccaaa attttcttga gcctgacctc ctttgccgtg ccgaccacgg
     1381 tgtattgctt tgagactgca acacaatcgc tcaccctttt tgaccagatt gagtccctgg
     1441 ttgatccagc cagctatatc atcaaacagg tcatttaccc atcgcgcgac cagaccaggg
     1501 tcagcatgtt tattgttcac cgcaaacatc ttaaattcga tgggatggca ccaaccattc
     1561 tgactggata tgggggattt aatatcagtc ggacccctga atttttagga tcgctgttga
     1621 cgttgctcga tgcgggtgct gtgtacgcat tgccaaatgt acgcggaggc ggtgaatatg
     1681 gcgaagaatg gcataaagcc gggatgcggg accggaaaca gaatgggttt gatgactttc
     1741 tctgcgcggc agaatacctg attcaacacc agtacacctg cccggaaaaa ctggcgattc
     1801 agggtgggag caatggtgga ttgctggtcg gcgccgcgtt gacccagcgc cctgatttgt
     1861 ttcgagccgt agtatgtcag gtgcccctgc tggatatgct gcggtatcac cattttttga
     1921 ttgcacgaac gtggattccc gaatatggtt gtgcagaaga ccaagacgcg tttcactggc
     1981 ttatggcata ctctccctac caccacgtca cgccagaaac gccatatcca gccgtattgc
     2041 ttatgaccgc ccagtccgat actcgggttg atccacttca cgctcgaaaa atggcggctc
     2101 gactgcaagc ggcaacatca tcagggtacc caattttgct tcgcgtcgaa tctcaggccg
     2161 gtcacggcgt tgggaaaccg accgccaaac gcatcgagga aagtgttgat gtgctgagct
     2221 ttatcgccgc tcagcttgga atttcgttgt aaggagttca gaccttgatt tggtatttga
     2281 aagcatcaca ggacaagccg ggacacgcca gacgtttcag agtgagttgg ttgggattga
     2341 gcctgctgct ggtactcacc ctcagcctcg ccacgggaat gacctcggcg tatcaaggag
     2401 ggggaggata cacattacag gttggttcaa ccaatgacga agctgcggct cgggcccagg
     2461 tggcccagct caaagccgcc ggtcataccg cttattatgt gcgtgccgag atccctggga
     2521 aaggcacctg gtatcggatt cgggttggtc gctttggatc gccagccgat gcccagcggt
     2581 attcacgtac cctgcctggg aaaagcacct ttgccacgtc ctatgatggt ccaaatggat
     2641 caccaaattc ccgcgtaccg tcctcagaac catcagccgt ggcgagtaaa acaccgaccc
     2701 catcaacacc ttcaaaacca aaatctgatc ctgccaaaat agcggccact acctcttcca
     2761 aacccgatac cagcaaacca tcaacggcta aagcaacacc caaagttgat ccagggaaac
     2821 cagccgcagg tacaacactc gggaaagccc agagtacctc cgtcaacaaa cccacgtctc
     2881 aaccgaaaac tgagattgcg aacaacacac cacccaggcc aaagaccgtt gaaactaaat
     2941 cttctgttcg agttcaacca aaaaataccc agccagcatt gaaattggaa acgccgaccg
     3001 tggcactggc cagtaaaatc tccaggcgac cccaattgct cgccctccca aaggagccag
     3061 cgaagtcagc tccggccccg acaacgactc agtcttccac gctgacagcc tctcgactcg
     3121 acccgaaaaa agggacctcg aagcctcagg aattgaattc aaaaactcca tcagatacct
     3181 cagctcgccc ctggattgct gaagtgagcc gcaaagaaaa aggcgaggaa gtcgcctccc
     3241 tgacgctgcc gaatttgggg aacccgctcc cgccggaaag tggagtgtca ggtggcgttg
     3301 gcctgaaact ggttgaagcg agcacgtcca aagctctgct cgaagcggcc ccggccaaac
     3361 cgcccgcgcc caaactggtc aagccccatc caccaatcaa gtttttgaca tcgctttctc
     3421 caaccccagc ctcgttcaag gccaaagacc tgggggcctt gctggcagca cctgaaacac
     3481 cgccaaccgg gtgggtcaga caatacccaa ctcttcgaac cgatttgacc cgcgtatttt
     3541 ttgccgatgc cgggcatggc tgggcggctg gcattcaagg catggtgctg tcaaccgaag
     3601 atggtggtga aacctggaat tcacagtcca ttggcgcccg tggcgttgtc aatgatttgt
     3661 tctttattga tcggctccgt ggctgggcgc tgatcggagg cacgctgggt actcaggcac
     3721 gggacttgcg cgatgaacag gtcctgtt
//
LOCUS       DAOQDO010000319         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17821_length_3738_cov_3.064893, whole genome shotgun sequence.
ACCESSION   DAOQDO010000319 DAOQDO010000000
VERSION     DAOQDO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17821_length_3738_cov_3.064893"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKZ53_06450"
     CDS             complement(<1..764)
                     /locus_tag="PKZ53_06450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ40112.1"
                     /translation="MRLVLFFLLSNVLGVIVASCYTRMPPPLSQPRGVLARELLKFEP
                     ALRTRSSLNEAAIQLRVVDRDHQLSSIFLQKLDAPPKDARLLVQELHTEILRLIQSEG
                     EQARAAGLHLEHLSWSMMALAAVNMLGFGISLHRIWHKHRNALELETQLERSQAELRT
                     TRSLATRQTEEVVLLRREHRNLREKLIAAVQEAELDPKGTGALTAKAGMRLLCQSLEL
                     NLGRVPVWVLIADADKFKNVNDTYGHRAGDIVLATLV"
     gene            complement(850..2223)
                     /locus_tag="PKZ53_06455"
     CDS             complement(850..2223)
                     /locus_tag="PKZ53_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNJ40113.1"
                     /translation="MNYPAVSALTPPDLLPDSTCLTSEDIQQLLSQLIETGVSDLHLR
                     GWHPPYQRNPNGELLLIEEAPVRVIPEPELFRLGYWLCRGTNYIQHLQALEQQFSRLH
                     YQVQRTESGQFESLEAHSDSFQTWITPEPFDIGVDVVSSEGRTGRFRVQIFFAESDSP
                     FEAGLNMAFRRILPAVPTHLDLGFCPAASHLISDLVSQKLTNGLVLVVGATGSGKSTS
                     VAAMLETLNEKVALNITTIEDPVEFKFHNKRSFFRQIGIRRHVPTYAQALNHVLRQDP
                     DIIVIQEIRDAEMARHALNLAQTNHLVIATLHGLTDTASALLQFVQMVGGDYLSVANH
                     LQAIVAQQLLPSKNRDAKRVLVQEVMRPRLNAAIQHLLRSHSADTDAYAGAIGRANEA
                     RSIEVRFVESLFTAVSHNEISAGRAFTATRYEADLLELFEARSPGNAISDYIPQYLQQ
                     RDRAEMA"
     gene            complement(2381..3646)
                     /locus_tag="PKZ53_06460"
     CDS             complement(2381..3646)
                     /locus_tag="PKZ53_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40114.1"
                     /translation="MYRTLFAGIIVLCGLCLTGGLTISEWQVARTQHILPAVLLVGKE
                     TPAQVDHLSHLRKILLSVDPETRQYPKTWAALNRLTRQSDQHGNLSTDPELAMSRHLA
                     TVDLFQFAAQEEVDTLTVQITASHFYTQLSSLVTLGIGVLFLTFIFYSERSIAKRLAT
                     LGTKGPRYSGQPDIRSHFDVSLSQQSKRVEDSFQELQFSVKQLQEQNLALVLAQRQRS
                     QSGPDQSPVVTQPLGTQVPDEVELQSAVGEFPSQTIPLASPRSTLSLVDVTPNTGEFQ
                     PAPEKIESPQLQTLKDIPLAPQGLNTYGGLLDLPYSDVVAIEIEELELLHHLYGHRVQ
                     MVIDLILNHLAESLQRHNVASHELAITGTTLYWALLEPTELSAIERFICDCYEQTPII
                     LEGNQINPPLLSVFFVESSQSSYRLVQFG"
BASE COUNT          902 a          908 c         1032 g          896 t
ORIGIN      
        1 accagggttg ccagtacaat atccccagcc cggtgaccat acgtgtcatt gacattttta
       61 aatttgtcgg catcggcgat taaaacccag acgggcaccc ggcccagatt caactccagt
      121 gactggcaca gcaaccgcat gccagctttg gcggtcagtg cacccgtgcc tttcgggtca
      181 agttccgctt cctggaccgc cgcaatcagt ttttcccgga ggtttcggtg ttcgcgccgc
      241 aacagaacaa cttcttctgt ttgccgggtg gccagtgatc gggtcgtgcg taactcggcc
      301 tggcttcgtt caagctgggt ttcgagttca agggcattgc ggtgtttgtg ccagatgcga
      361 tgcaagctga tgccaaatcc cagcatattg acggctgcca gggccatcat gctccaggaa
      421 agatgttcaa ggtgaagacc ggctgcccgg gcctgttccc cttcagattg aatcagtcgc
      481 agaatctcgg tgtggagttc ctgaaccagg agccgggcat ctttcggtgg ggcatccagt
      541 ttttgcaaaa atatagagct gagctggtga tcacgatcaa cgacccggag ctggattgcg
      601 gcttcgttga gactggatcg agttcgcaag gctggttcaa attttaacaa ttctctggcc
      661 agtaccccac ggggctggga aagcggtggt ggcattcggg tatagcacga tgccacaatg
      721 acccccaaaa cattgctcaa aagaaaaaat aataccaggc gcatagcgtt ttccttcggt
      781 cggtggaatg agttcattgg cgtcggtcaa gcgaaaaagt gaggtacccg ccaggaaaat
      841 cagttcaatt caggccattt cagctctgtc gcgttgttgc aggtactgtg ggatgtaatc
      901 tgaaatggca ttgcccggtg acctggcttc aaagagttca agtaagtcag cttcatatcg
      961 cgtggcggta aaggcacgcc ccgctgaaat ttcattgtga ctcaccgccg taaacagtga
     1021 ttcgacaaag cgaacttcaa ttgacctggc ttcattggcc cggccaattg ccccagcata
     1081 cgcgtcggta tcggctgagt ggctgcgcaa caggtgctga atggcggcat ttaaccgggg
     1141 gcgcataact tcctggacca ggactcgttt ggcatcgcga ttttttgacg gcaagagctg
     1201 ctgggcgaca atcgcctgta aatgattggc aactgacagg taatctccgc caaccatctg
     1261 cacaaactgc aacagcgccg aggcggtatc agtcaatcca tgcagggtgg caatcaccag
     1321 atgattggtt tgcgccaggt tcaaggcgtg acgagccatt tcagcatcac ggatttcctg
     1381 gatcacaatg atgtccgggt cctgccgcaa aacgtggttg agtgcctggg cataggttgg
     1441 cacatgacgg cggatgccga tctggcgaaa aaacgagcgt ttgttgtgaa acttaaattc
     1501 aaccgggtct tcaatcgtgg tgatattgag cgccactttt tcattgagtg tttcaagcat
     1561 cgccgctacc gaggttgatt ttcctgatcc ggtggctccg accaccagca ccaggccatt
     1621 ggtgagtttt tgcgacacca ggtctgaaat caaatgactg gctgccgggc aaaacccaag
     1681 atcaaggtga gtcggaacgg ctggtaaaat tcggcggaac gccatattca acccggcttc
     1741 aaacggggaa tctgattcag caaaaaaaat ctgtacccgg aaccgccctg tgcgtccctc
     1801 tgatgaaacc acatctaccc cgatgtcaaa cggttcgggt gtgatccagg tctggaatga
     1861 gtctgagtgg gcctccaggc tttcaaattg accggattca gtccgttgaa cctggtagtg
     1921 caagcgggag aattgctgtt cgagagcctg caggtgctgg atgtaattgg ttccgcggca
     1981 caaccagtat ccaagtcgaa agagttctgg ttctggaata acccgcaccg gggcctcctc
     2041 aattaaaagc aactctccat ttggattccg ctgatatggc ggatgccacc ctcgcaggtg
     2101 cagatcactc actccggttt caatcaattg actcaggagt tgctggatgt cttccgaggt
     2161 caggcacgtg ctgtcaggaa ggaggtcagg aggtgtcaga gcggaaactg cagggtaatt
     2221 cacggtactc tctccttgag tgaggtcaat tcagtgaatg aaaatgggtt cctgattatc
     2281 ccaaatggca gtgaacccgt tggattagaa aatggtcaaa taattgaatc aaatgagctt
     2341 tatgaccaac tgactctggg cgactgattc caaaccaggg ttatccaaat tgaaccaggc
     2401 gataacttga ttgagatgat tcaacaaaga agaccgagag caacggcgga ttgatctggt
     2461 ttccctccaa aatgatcggt gtctgctcat aacaatcgca aataaagcgt tcgatggcgc
     2521 ttaactcagt tggctccaaa agtgcccaat aaagcgtcgt cccagtgatc gcaagctcgt
     2581 gtgaggcaac gttgtggcgc tgcaagcttt cagccaggtg gtttaaaatc aggtcaatca
     2641 ccatctgaac tcgatggccg tacaggtgat gcaacagttc aagttcttca atttcaatcg
     2701 ccaccacatc gctgtagggg agatccagca ggccgccata ggtattgagt ccctgcggag
     2761 ccagcgggat gtcttttaag gtttgaagtt gtggggactc tattttttct ggagcgggct
     2821 ggaattcgcc agtattaggg gtgacatcaa ccagactgag cgttgatctg ggggaagcca
     2881 gcggtatggt ttgagagggg aactcaccga cggcggattg aagttcaact tcatccggta
     2941 cctgggtacc caatggctgg gtgacgactg gtgactggtc aggtcctgac tgagaccgct
     3001 ggcgttgagc cagaaccaga gcaagatttt gttcttgaag ctgcttgacc gaaaactgaa
     3061 gctcttgaaa tgagtcttca actcgctttg attgctggct taacgaaaca tcaaagtggc
     3121 tccggatgtc aggctgacca ctgtatcggg ggcctttcgt tcccagcgtt gccaatcgtt
     3181 tggcgataga tcgttctgaa taaaaaatga aggtcagaaa cagaacccca attccgaggg
     3241 tgaccagtga actcagttgg gtataaaaat gtgaggcggt gatctgaacg gtcagggtat
     3301 ctacttcttc ctgggcagca aattggaaga gatcaacggt cgccagatgc cgcgacattg
     3361 ccaactctgg atctgtcgac aggtttccgt gctggtctga ctggcgggtc agtcggttca
     3421 gagctgccca tgttttcgga tactgtctgg tttcagggtc taccgacagc aatatttttc
     3481 tcaggtgtga cagatggtcc acctgcgctg gtgtttcttt tccgaccaga agcacagccg
     3541 gaagaatgtg ttgtgtcctg gcaacctgcc attcagagat ggtcagtcca ccagtcaggc
     3601 acaggccgca taaaacaatg attccagcaa aaagggtccg gtacatgggg ttgctcctgc
     3661 gaggggattt acattgatgc tgaccctcga aaaagcaaat gaagtgccac acagcctggg
     3721 atagtccagg ctttagtg
//
LOCUS       DAOQDO010000320         3732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17865_length_3732_cov_4.285831, whole genome shotgun sequence.
ACCESSION   DAOQDO010000320 DAOQDO010000000
VERSION     DAOQDO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17865_length_3732_cov_4.285831"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..438)
                     /locus_tag="PKZ53_06465"
     CDS             complement(43..438)
                     /locus_tag="PKZ53_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ40115.1"
                     /translation="MGQKACKPELLFQALADRTRLRLLNLMGDDEVCVCFLVAILGTN
                     QPKISRHLAYLRKAGVVEVRRDGKWAHYRIVKPEDPIAASVLDDVRRWIAQDSEMQKD
                     RFSLVNLCCAPQLPIQLRGAPRPANLATP"
     gene            575..1042
                     /locus_tag="PKZ53_06470"
     CDS             575..1042
                     /locus_tag="PKZ53_06470"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNJ40116.1"
                     /translation="METPRPTALKAHLAINVTDVTQSIEFYKKMFGIEPLKVRTGYAK
                     FDVANPPLNFTLNQVPFGERGALSHMGIQVSSTEDVLALRQNWIDSGLEVRDEMQTNC
                     CYAIQDKTWVKDPDGNEWEVFVVLQDQLAESTTCCVPETVTLGSKKKAGVCSI"
     gene            1272..2318
                     /gene="arsB"
                     /locus_tag="PKZ53_06475"
     CDS             1272..2318
                     /gene="arsB"
                     /locus_tag="PKZ53_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNJ40117.1"
                     /translation="MSQSITHQLSFLDRFLTLWIFLAMAIGVLLGYFVPGVEQVINRF
                     QVGTTNIPIALGLILMMYPPLAKVRYEELGDVFRDWKMLTLSLVQNWIIGPLLMFGLA
                     IVFLRDYPEYMTGLILIGLARCIAMVIVWNELACGDTECAAGLVAFNSIFQVLFYSVY
                     AWVFLTILPPYFGLKSVVVNISISQIAESVFIYLGIPFLAGMMTRLVLVKAKGKAWYH
                     STFVPVISPITLVALLFTILVMFSLKGNLIVQLPLDVVRVAIPLLIYFVVMFFVSFFM
                     GHRVKTGYAKTATLSFTAASNNFELAIAVAVAVFGLNSGAAFAAVIGPLVEVPALIAL
                     VNVALWLQQKYFSS"
     gene            2442..2864
                     /locus_tag="PKZ53_06480"
     CDS             2442..2864
                     /locus_tag="PKZ53_06480"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNJ40118.1"
                     /translation="MHQPLRTLILCTGNSARSQMAEGLLRHDGGAAFEVHSAGTKPSL
                     VRPEAIAALREIGIDITSHWSKSVNDFAGQEFDYVITVCDNAKENCPYFPAKTRRIHF
                     SFDDPAGVTGTDDERLAEFRRVRDEIRTWLAGFIQEVQ"
     gene            2871..3434
                     /locus_tag="PKZ53_06485"
     CDS             2871..3434
                     /locus_tag="PKZ53_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40119.1"
                     /translation="MEHLEIAAASSLALFSIFAAVDGIYLHLWKYRLHARPETVFEHK
                     LHTLRAFLFIPTLYLLFVNNSAGIALWLGLAFVLIGFVVEFWDVLSENESRKELGGLS
                     SIEYATHVVITIFQVLALAFLFASKPLAAWSLDAGVLLPTSYTPFLKMTGINTLIGTI
                     GMAGLHVWLLRPKYRQGNRLIVVQTCC"
BASE COUNT          897 a          916 c          911 g         1008 t
ORIGIN      
        1 ggaacccgga acccggaacc cggaacccgg aacccgatgg ttttatgggg ttgcgagatt
       61 cgccgggcgt ggggcgccgc gcaattgaat cggtaattgc ggtgcacagc acagattaac
      121 gagtgaaaat cggtcttttt gcatttctga atcctgggcg atccatcttc ttacatcatc
      181 caggacgctg gcagcaatcg gatcttcggg tttgacgatt cgataatgcg cccatttgcc
      241 atcccgccgc acttccacca cgccagcttt gcgcaaatac gccagatggc gcgagatttt
      301 cggctggttg gtgcccaaaa tcgccaccaa aaaacaaaca cagacttcgt catctcccat
      361 taagttcaac aaccgcaacc gggtacggtc agccagggcc tgaaacagca attccggttt
      421 acacgctttt tgtcccattc ggacacctcc gcaaaaaaat ttcaacatat acgcctaaac
      481 gtatcaaata tgtcttgaca tatatgaacg tcgggcgtat tgtcgcattc gcttaaacga
      541 atatgaaata caacagaaag gaattcaact cattatggaa actccacgtc cgaccgcgtt
      601 aaaagctcac ctggctatca atgtcaccga tgtgacacag agcatcgaat tctacaaaaa
      661 aatgtttggg attgaacccc tgaaagtgcg cactggctac gccaagtttg atgtcgccaa
      721 cccaccgctc aatttcacgc tcaatcaagt tccctttggc gaacgtggtg cgctgtcgca
      781 tatgggcatc caggtttcat caactgaaga cgtgcttgcc ctgcgccaaa actggattga
      841 ttccggtttg gaagttcgcg acgaaatgca gaccaattgc tgctacgcca tccaggataa
      901 aacctgggtc aaagacccgg atggcaatga atgggaagtt tttgtggtgt tgcaggatca
      961 actggctgaa agcacgacct gctgtgtccc tgaaaccgtg acgctcggca gcaagaaaaa
     1021 agccggggtg tgctcaattt gaggatgaag cctttgggct gaagaagtcg ggcttggggc
     1081 tgaagaagat gagctgagaa aggtcagaat gttcgcatgc gaacaattcg tgcaatgtgt
     1141 taagaataaa tcatttaacc cagattgata aatttctctc ggtctgcaat tctttttcaa
     1201 aatcctcagt cttcaaccca acttcttcag ccctcagccc caagcccgaa gtcttccgcc
     1261 cgagaatgcg tatgtctcaa tcaattaccc accagctctc atttcttgac cgatttttaa
     1321 cgctctggat ttttctggcc atggccatag gtgttctgct tggatatttt gtgccgggtg
     1381 tggaacaggt gatcaatcgg tttcaggttg gaaccaccaa tattccgatt gcgctggggc
     1441 tgatcttgat gatgtaccca ccactcgcaa aagttcggta tgaggagtta ggcgatgtct
     1501 tccgggactg gaaaatgctc acgctgtcgt tggtccagaa ctggatcatc ggtccgttgc
     1561 tgatgtttgg actggcaatc gtctttttgc gggactaccc ggaatatatg accggtttga
     1621 ttttgattgg actggcgcgg tgtatcgcca tggtcattgt ttggaacgag ttggcctgtg
     1681 gagacaccga atgcgccgcc ggactggtgg cctttaatag catttttcag gtgttgtttt
     1741 acagtgtcta tgcctgggtt tttctgacga ttctgcctcc gtattttggc ctcaaaagcg
     1801 tggtggtcaa catcagcatc agccagattg ctgaaagtgt ctttatttac cttgggatcc
     1861 cgtttctggc ggggatgatg acccgcctgg ttctggtcaa agccaaagga aaagcgtggt
     1921 atcactcaac ctttgtgccg gttatcagcc cgattaccct ggttgccttg ctctttacca
     1981 ttctggtgat gtttagcctc aagggaaatt tgattgtgca attaccactg gatgtcgtgc
     2041 gggtggccat cccattgttg atttactttg ttgtgatgtt cttcgtcagc ttctttatgg
     2101 gccatcgtgt caaaactgga tatgccaaaa cggcaaccct ttcatttact gccgcgagca
     2161 acaactttga actggcaatt gccgtggccg tggccgtgtt tggcctgaat tcgggggcgg
     2221 cctttgccgc agtgattgga ccactggtcg aagtaccagc gttgattgca ctggtcaacg
     2281 tcgccctctg gctgcaacaa aaatattttt caagctgaga aaaccggggc tgagggcttg
     2341 gggctgaaga ccttgggcta aggctcaggg tcttgaaccc agtttcttca gccctgagct
     2401 tgcgagtctt tcagccccaa gccctcagcc cggagccctc tatgcaccaa ccacttcgaa
     2461 cactcatcct ctgtactgga aattccgccc gcagccagat ggctgaaggg ttactccgcc
     2521 acgatggcgg cgcggcattt gaagtccatt cggctggaac caaaccgagt ctggttcgcc
     2581 cggaagcaat tgccgccctc cgtgaaatcg gtattgacat caccagtcac tggtcaaaat
     2641 cggtgaatga tttcgccggg caggaatttg actacgtgat tactgtgtgt gacaacgcca
     2701 aagaaaattg tccttacttt ccagccaaaa cccggcgcat ccacttcagt tttgacgatc
     2761 cggctggcgt cacggggact gacgacgaac gcctggctga atttcgacgt gtgcgtgatg
     2821 aaatccgtac ctggctcgct ggttttattc aggaggttca ataagcaact atggaacact
     2881 tagagattgc cgccgccagt tcactggccc ttttttcaat ctttgccgcc gttgacggca
     2941 tttatttgca cctgtggaaa tatcgcctcc atgcccggcc agaaactgtt tttgagcata
     3001 aattgcacac attgcgggca tttttgttta ttccaacgct ctatttgctg tttgtgaaca
     3061 attccgccgg gatcgccctc tggctggggt tagcgtttgt attaattggc tttgtcgttg
     3121 aattttggga tgtactctct gaaaacgaga gccgaaagga actgggcggg ctgtcctcaa
     3181 ttgaatatgc cacacacgtt gtgattacta tttttcaggt attggctctg gcattccttt
     3241 ttgccagcaa accgttggcc gcgtggagcc tcgatgctgg cgtgctgttg ccaaccagtt
     3301 acacaccatt tctgaagatg actggtatca atacattgat tggaaccatt gggatggccg
     3361 gcctgcacgt ctggctcttg cgccccaaat atcgtcaggg gaaccgcctg attgtggttc
     3421 aaacctgctg ctaaaaagaa tgaagaatga agaatgaaga gtgaagaatg aagagtgaag
     3481 aatgaagaat gaagaaaatt gagtgtttgg ttcaaaaaag tttccatgac aacatttcta
     3541 taaattaaat gtaatcatag aattacagcg aaaaacttgg ttttccgtac aaacttacct
     3601 gctagcataa aatatgccct aagctattct tacaaaattg gttaaggcaa ttcttcacgc
     3661 ttcacgcttc attttttatt cttcacgctt cattccccaa atttcgccca aaatactgac
     3721 ttgttcccca gt
//
LOCUS       DAOQDO010000321         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17875_length_3731_cov_2.355822, whole genome shotgun sequence.
ACCESSION   DAOQDO010000321 DAOQDO010000000
VERSION     DAOQDO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17875_length_3731_cov_2.355822"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PKZ53_06490"
     CDS             complement(<1..1128)
                     /locus_tag="PKZ53_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40120.1"
                     /translation="MTSFVTTAVHFRSLLLVSALILCSMVSVTAQEPLELDVTKYGVV
                     YRIPEMDQVKVRRNLQYGSIGGRPKHFDLYLPPQTRSSELPPVIVFINGVGDFGDNKL
                     KDWTIYMDWGKLAAAKGFAAINFETDHERVGESIAAMFTYLDQHGKEQGIDATRIGTY
                     ACSGNVGQALPYLMGTPVKGIRAAVVYYGAAQVESIRTDLPVMHVLAGRDNPNLLQFQ
                     RRLWDQVRAKQAPWAMIESPTLPHAFDGIYYGEESRRIIHQTLAFWESHLQPLPAPPP
                     AKAGQEIVESFYANDWGKAVPLLSEAVKKYPNRADVWGQLGRAHQNTGNFPEAEKVLL
                     KAAELEPTNAGTLRTLGLVQERQQKYAEASKTFQRAIELGGN"
     gene            1649..1867
                     /locus_tag="PKZ53_06495"
     CDS             1649..1867
                     /locus_tag="PKZ53_06495"
                     /inference="COORDINATES: protein motif:HMM:NF021362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HNJ40121.1"
                     /translation="MKAVSYRLISSMITGGIVFGATQQGLLALSVAGGETIIKVIVFF
                     LHERAWSAIPLGKIEKNTELEELISVGE"
     gene            2043..3641
                     /gene="cobG"
                     /locus_tag="PKZ53_06500"
     CDS             2043..3641
                     /gene="cobG"
                     /locus_tag="PKZ53_06500"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR02435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HNJ40122.1"
                     /translation="MAKKETRAEKVKRERDALDCLDDLLRYAKEGFAAIDPDDLDVRL
                     RWYGLYTQRPQEDGYFMLRVKIPNGTLTVEQLATLGQLSVRYGQNTGDVTTRQDIQFH
                     YICIEDVPAIFEALNRVGLTTSGACGDITRNVTGCPLAGIDSSELIDAEPIIEQIHHY
                     FLDNHQYSNLPRKFKITVSGCSQYCTGHEINDVGLIAVRRPDGRVAFDLWVGGGLGSK
                     ERFADRLGVHVFPEEAAEVTAHVCALFRDHGFREYRHKARLKFLLEAWGPARFRQELE
                     ARLGRQLIDGEAPSIPVNANRAHIGIRAQKQAGLHYAGAATERGRLNGTEMIALANVA
                     KRYGSNRIRLTTTQNLVVLDVPEQVSQSLSQELAALGLFTNASVFRTGTIACTGKRFC
                     KLAVVETKARASELIEYLEATVPEMKVPLRISVTGCPNSCAHYQVCDVGLVGDHIVTP
                     AGKIEAFRVFLGGYLGNDYRFGQELSTKVAAEEVKYYLERVVRDYLAHRISPAESFQA
                     FISRQSLEELEHLGSFEERAVLTT"
BASE COUNT          930 a          919 c          933 g          949 t
ORIGIN      
        1 gttgccgccg agttcaatcg cccgctgaaa cgttttcgag gcttcggcat acttttgctg
       61 gcgctcctga accagaccca gggttcgaag ggttccagcg tttgtcggct caagttcagc
      121 cgctttcagg agcacttttt cggcttcagg gaaatttccg gtattctggt gagcccgtcc
      181 cagttgtccc cacacgtcgg cgcgattcgg gtactttttc accgcctctg acaacaatgg
      241 cacagccttg ccccagtcat tggcatagaa tgattcgaca atttcctgac cggctttggc
      301 tggaggaggt gctggcaagg gctgtaaatg actttcccaa aaagccagtg tctggtgaat
      361 aatccgacgg ctctcttcgc cgtaataaat accatcaaat gcgtgcggaa gcgtcggcga
      421 ctcgatcatc gcccatggtg cctgtttggc tcgcacctga tcccataatc tccgctgaaa
      481 ttgaagcagg ttcggattat ctcttcccgc caggacatgc atcacaggca aatcagtccg
      541 aattgattca acctgggcgg caccataata aaccacagcc gcccgaatcc ccttgactgg
      601 cgttcccatc aggtaaggga gtgcctgtcc aacattgcct gaacaggcat aggtgccgat
      661 tcgtgttgcg tcaataccct gttccttccc atgctggtca aggtaggtaa acattgccgc
      721 gattgactca ccgacacgtt cgtggtccgt ttcaaaattg atggcggcaa accctttggc
      781 tgccgccagt ttgccccaat ccatatagat ggtccaatcc ttcaacttat tgtcgccaaa
      841 atcaccgaca ccattgataa acacaattac cgggggtagt tccgaagacc tggtctgagg
      901 tggcaggtag agatcgaaat gttttgggcg gcctccaata gacccatatt gaaggttgcg
      961 gcgcactttt acctgatcca tctctggaat tcggtaaacg accccgtatt tggtaacgtc
     1021 aagttccaat ggttcttgtg ccgtcaccga caccatcgag caaagaatca gcgctgaaac
     1081 aagcaacagt gagcgaaagt gaaccgcggt agtcacaaag gatgtcatat ccctctccag
     1141 tatttgaccg atttttttgg gaattgtgtc agcctgagtt gacgagaacc tttttagaca
     1201 ggaagcaact tgaatttctt ccattatttt tggagtgcga cggcttgacg tgctgaagaa
     1261 tcacccctca ggaaaaaaga gttgacgttt ctgaaactat actgtacaaa atcgtcacta
     1321 tattgacagc tcgcagttga ttgttttcaa aaattttcta cgtggtgatt tatggttact
     1381 tgctccacgc taaaaagtgc taaagaaccg ctcgacgata cctgtgttga cctcgtgcgc
     1441 ttcttcaaag cacgaggttt tttcgttcag gaagatttct ttatgagcca aatcaggatg
     1501 ctgttgcacg gacgagccca aaagtccacc tggtgttgtt gccgctgaca aacggcacat
     1561 aagttttcat ttcgaaggct taaaaaaatc cctttttgtt tcattgttaa tttctttcac
     1621 ttgtatggaa acacattaca gaagtcttat gaaagcagtg agttaccgac tcatttcctc
     1681 aatgattacc ggaggaattg tctttggtgc aactcaacaa ggattactgg cactcagcgt
     1741 tgccggaggt gaaaccatca tcaaggtgat tgtttttttc ctgcacgaac gggcctggag
     1801 tgcaattcca cttggaaaaa ttgaaaaaaa caccgaactc gaagaactga tctcagttgg
     1861 agaatgagga accagagccg aatgaagaat tgagaatgaa gaatgaagaa aatatgagtg
     1921 gttagcggtt agtggttgga aatcaatact ttcgaagaag aaccaggagc caggtttctg
     1981 cattctcaat tcttcattct tcattcttca ttctcaattc ttcattccaa aaggaggttg
     2041 ccgtggcaaa gaaagaaacc cgtgccgaaa aagtgaaacg cgaacgtgat gcccttgatt
     2101 gtttggatga cctgctgcgg tatgcaaaag aaggttttgc cgcaattgac ccagatgatc
     2161 ttgacgtgcg gttgcgatgg tatggcctgt atacccaacg tccacaggaa gatgggtatt
     2221 tcatgctccg cgtgaaaatt cccaatggca cactcacggt tgaacaactg gcaacgctgg
     2281 ggcaactttc ggttcggtat ggacaaaata ctggcgatgt tacaacacgc caggatatac
     2341 aatttcatta catttgtatc gaagatgtac ctgccatttt tgaagcgtta aatcgtgttg
     2401 gattaaccac ttccggcgcc tgcggagaca tcacccgcaa tgtaactggt tgcccgctgg
     2461 ccgggattga ttccagcgaa ctgattgatg ccgaaccaat tattgagcaa atccatcact
     2521 attttctcga taaccaccaa tattccaacc taccacgaaa gttcaaaata acggtttccg
     2581 gttgctcaca gtattgcacg gggcacgaaa tcaatgatgt tgggctgatt gcggtccgcc
     2641 gcccagatgg tcgagttgcg tttgacctgt gggttggtgg cgggcttggc tcaaaagaac
     2701 gatttgccga ccggctcggc gtgcatgtct ttcccgaaga agcggctgaa gtaacagccc
     2761 acgtttgcgc tctgttccgc gaccacggct ttcgtgaata tcgccataaa gcgcggctca
     2821 agtttttgct cgaagcctgg gggccagccc ggtttcgcca ggaacttgaa gcccggctgg
     2881 gccgccagct tatagacggc gaagcgccgt ccatcccggt caatgccaat cgggcccaca
     2941 ttgggatccg ggctcaaaag caagctggac tccactatgc tggcgcggca accgaacgcg
     3001 gacgactcaa cggaaccgaa atgattgccc tggccaatgt tgccaaacgc tatgggagca
     3061 atcgcatccg gcttacaacc acccaaaatc tggtcgtgct ggatgtaccg gaacaggtca
     3121 gtcagtcact ttcacaggaa ctggcggcgc ttgggttatt taccaatgca tcagtctttc
     3181 gaactggcac cattgcctgt accgggaaac gcttttgcaa actggccgtg gttgaaacca
     3241 aggcgcgagc ttcggaattg attgaatacc ttgaagcgac cgttcctgag atgaaagtcc
     3301 cgttgcgaat cagcgtcacc ggatgcccga acagttgtgc ccattatcag gtgtgtgatg
     3361 ttggcctggt cggagatcac atcgtgacgc cagccgggaa aatcgaagcg tttcgcgttt
     3421 ttctgggcgg ctatttaggc aatgactatc ggtttggaca ggagttgtcc accaaagttg
     3481 cggctgaaga agtcaagtat tacctcgaac gggtggttcg tgactacctg gcgcatcgca
     3541 tcagcccggc ggaaagtttt caggcattca tttcccgcca aagtctggaa gaactcgaac
     3601 acctcggcag ttttgaggag cgagcggtac tcactacctg atgcccactg gaataccaat
     3661 tagggttcag ggttcagggt tcagggtttg aggaattata ccattttgca gtgaagttct
     3721 catattacgg g
//
LOCUS       DAOQDO010000322         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17892_length_3729_cov_3.350844, whole genome shotgun sequence.
ACCESSION   DAOQDO010000322 DAOQDO010000000
VERSION     DAOQDO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17892_length_3729_cov_3.350844"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1142
                     /locus_tag="PKZ53_06505"
     CDS             231..1142
                     /locus_tag="PKZ53_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNJ40123.1"
                     /translation="MKSFSCWLLIFVLIGLSSTGSAQNHRSAKLTAYPPDALAELEKW
                     RAEYESDVKSETGWLSIRGLFWLKPGANLVGSAADNVVVLPAEAAPARVGVITLDGKK
                     ARLAVQAGVPVAVNGKAVTETDLKTDADGKADMITLGTLSFYIIVRGEKIGVRLKDTN
                     NPALRNFPGVHWFEPNGAFRVEARFVPYNPPKILKITNVLGDVSDVPSAGYVEFRLLG
                     KKCRLDVQQADDRLFVNFKDRSAGVTTYGAGRFLEPELPKNGKVMIDFNRAYNPPCAY
                     TEFATCPLPPPQNALPVAIEAGEKKTH"
     gene            complement(1667..2080)
                     /locus_tag="PKZ53_06510"
     CDS             complement(1667..2080)
                     /locus_tag="PKZ53_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40124.1"
                     /translation="MPINNAAQLQELIKNELSTIPLEKWRYSFPCSVAVWRFIESQFP
                     GVFGPLQAGFDTEPFFIGKGAKWFCDVLQNDEAYLDLLESILYEVVRKLGKHSKERGF
                     LIRAEVPELNGETINHLAQVIVSKVEDGIKNSQIE"
     gene            2628..3200
                     /locus_tag="PKZ53_06515"
     CDS             2628..3200
                     /locus_tag="PKZ53_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ40125.1"
                     /translation="MLYRQIMDYELLQRARTGDLQAFDQLYQRHKVVVWQFVLSRVGS
                     PMTAEDIVHDTFLELIKHPSRFDPYRGVLFEAYLCAVAKKLILKHQRKRRRETLVSDQ
                     SVTPICFNQEEKPTFEQRANQKIDVQRALKNLAFNHRQVLQMVEIEGYSVKELSDQAG
                     LSIVALKARLHRAKLALRKLLITEARFGSK"
     gene            complement(3249..3641)
                     /locus_tag="PKZ53_06520"
     CDS             complement(3249..3641)
                     /locus_tag="PKZ53_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40126.1"
                     /translation="MSDPCPPIEIVLSDSIDRILRFEQDLLLFLLSAIQTYLAERTAD
                     GASPAHRHTTQLKILESIKLLHLYQLIECHPEIDFSNSDHRLKLEKIVSAGFATIQKN
                     VEREFLPTSTSTYVSDPSDEAPPCRRLH"
BASE COUNT          958 a          866 c          916 g          989 t
ORIGIN      
        1 gggcggaaga ctcgcacgct cggggctgaa gaaggagggt acttcaacct gatttcttca
       61 gccccaagcc ccaagcccca agcccgttgt cttcagccct cagccctggt tttttcagtg
      121 cgatgttttt tgattttttc gtgtgtttca tggttaaaaa tgtctttgaa tttccgctga
      181 catctggcta ccttcaaccc acgatcccaa actcatctct ggaaggtttt atgaaatctt
      241 tttcctgctg gttgctcatt tttgtcttga ttgggttgtc ttcaacaggt tcggctcaga
      301 atcacaggtc ggccaaattg accgcctatc cgccggatgc gctggccgag cttgaaaaat
      361 ggcgtgctga atatgaatca gatgtgaaat ctgaaactgg ctggctttca attcgagggt
      421 tgttttggtt aaagcccggt gcgaatttgg ttggaagtgc ggctgataac gttgtggtat
      481 tgccagccga agctgcacca gctcgggttg gtgtgattac actcgatggc aaaaaagccc
      541 gactggcggt tcaggctggc gtaccggtag ctgtcaacgg gaaggctgtc accgaaacag
      601 acctcaaaac cgatgctgat ggcaaagccg atatgatcac gctggggacg ctctcctttt
      661 atattattgt gcgtggagag aaaattggcg tccgactcaa agacaccaat aacccggcac
      721 tccgaaattt ccccggtgtt cactggtttg aacccaacgg tgcttttcgg gttgaggcca
      781 ggtttgtacc gtacaaccca cccaaaatct taaaaatcac caatgtgctg ggcgatgtga
      841 gcgatgttcc aagcgccgga tacgttgagt tccgcttact tgggaaaaaa tgccggctgg
      901 atgttcaaca ggccgatgat cggctctttg tcaatttcaa ggacaggtcc gccggcgtga
      961 ccacctatgg cgccggtcgg tttttagaac cagagcttcc caaaaacggc aaagtgatga
     1021 ttgatttcaa ccgggcctac aatccgccct gtgcctacac tgagtttgcc acctgcccgt
     1081 tgccgccacc gcaaaatgca ctgcccgtgg caattgaagc cggagaaaag aagacacatt
     1141 gaagatcttg ggtgcggagc ggaagcggcc tggtatcagt agccgtaacc gattgatgac
     1201 cacgacgatt ggacagaaga tgatctgcgg gaagcaatgc tggtttcctg gagctacttg
     1261 gaagtaagag gcgaaagcca gaaagctggc ggccaaactc agggaactgg gacttgaccc
     1321 cgacgccttg taaaaaggtg ggtgatgcct tcatctcaaa agcgcacggt tgagccattg
     1381 accaccagcg aaagacagtt caaccgaaag gaaaaccaat ggctggaatc aactacattg
     1441 tggatgaaca gtgaaaccgg acggcggtgc tgctgccctt acccaaatat ggcgctctgc
     1501 ttgagcaatt gctgggggat gctgagtgag gccgtctggt tcgggaaatc ccacctgacc
     1561 agtgccccac aggcattgaa gccaatcagt tcctggtctt gaggatgctg atgatgaatg
     1621 attttttatg gatcaaccct ggcctacttt ctacttttct ttagatttac tcaatctggg
     1681 agtttttgat cccatcttca actttggaga caataacctg ggcaaggtgg tttattgttt
     1741 caccattcaa ttcgggaacc tctgctctta ttaaaaatcc tctttctttt gagtgttttc
     1801 ctaattttct aacaacttca tacagtattg actcaagaag atccaaatat gcttcatcgt
     1861 tttgcaatac atcacaaaac catttggccc ctttgcctat aaaaaagggt tctgtatcaa
     1921 atccagcttg gagaggtcca aacacacctg gaaactgtga ttcaataaac ctccacacgg
     1981 caacgctaca cgggaaagag taccgccact tctctagtgg tattgtagaa agttcattct
     2041 ttatcaactc ttgcaattgt gctgcattat ttatgggcat caggtaaccc ccaaaattct
     2101 aaggtttcag ctaattcaac tccaactcag gctcaccgct cccagcagat atatttttca
     2161 ccttttgctg aaatattttt aaccaaaagg tggagccggg taaagtattg atttactgaa
     2221 ggatatccag gttttatcct ctttgtgtct ttcgggtctt tcaccttttg ggtaaaacct
     2281 cttcagatga atgactaatg cctggttgtc ttgagatgtg gatgctcatt caatgggcgc
     2341 catatcgggt aactggtccc gccaaagcca ccaggaccag tttcaaatca agacctgcca
     2401 tccagaaacc gctctgaatc caaaccaggt atcactgccc tttaacgaaa ccaggctgcc
     2461 agggccttta gcttgttttg agcgtcttcc gataatctca tttccagtat tcataccaac
     2521 ccatcaccta ccgagtagga ggattatctt gaattcaaag ataatcttct tcaacctgaa
     2581 tcctggctgg tcaaagtggt gccgtgactt ccacacgaat tcctttggtg ttatatagac
     2641 agattatgga ttatgaattg ctccaacggg ccagaaccgg tgatcttcag gcgtttgatc
     2701 agctctatca gcgtcataag gttgtcgtgt ggcaatttgt gttaagtcgg gtcgggtcgc
     2761 cgatgactgc cgaagatatc gtccacgata ccttcctgga attaatcaaa catccgagcc
     2821 ggtttgatcc atatcgcgga gttctatttg aggcctattt gtgcgccgtg gcaaaaaaat
     2881 taatcctcaa gcatcaacgc aaacggaggc gggaaacact ggtgtcagac cagtcagtca
     2941 caccgatatg tttcaaccag gaggaaaaac caacttttga acagcgtgcc aaccagaaga
     3001 ttgatgtaca gcgggcactg aaaaatctgg ctttcaatca tcgtcaagtc ttgcaaatgg
     3061 ttgaaattga aggatattcg gtcaaagaac tttcagatca agccggattg agtattgtcg
     3121 cgttgaaggc caggttgcat cgggccaaac tggccttgcg gaaactgttg attaccgagg
     3181 cccggtttgg aagcaaatga ccaggctggt ggcggctggc ggagacctca ttgtgagaaa
     3241 tttcatattt agtgcagcct ccggcagggt ggtgcttcat cggatgggtc agaaacatag
     3301 gtcgaagtcg aagttgggag aaactcccgc tcgacgtttt tttgaatggt ggcaaaaccg
     3361 gcagaaacga ttttttccag tttgagacga tggtcgctgt tggaaaagtc aatttcggga
     3421 tggcattcaa tcagttgata taaatggagc agcttgatgc tctccaggat tttcaattgg
     3481 gtggtgtgac ggtgtgcagg acttgcccca tcggcggttc gttcagccag ataggtttga
     3541 atcgccgaga gtaaaaataa cagcaaatct tgctcaaaac gcaggatacg gtcgattgag
     3601 tctgacaaaa caatttcaat cggcgggcag ggatcagaca cgatagattc ctccgttgat
     3661 gtggactcac gataggaact tggattttcg gtagcttgaa aaaaaagagg tcttctataa
     3721 tttccactt
//
LOCUS       DAOQDO010000323         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17910_length_3727_cov_3.019608, whole genome shotgun sequence.
ACCESSION   DAOQDO010000323 DAOQDO010000000
VERSION     DAOQDO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17910_length_3727_cov_3.019608"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PKZ53_06525"
     CDS             complement(<1..1583)
                     /locus_tag="PKZ53_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNJ40127.1"
                     /translation="MKDVEIIEPAEPGRKPPRIPSPATAVNQGEGQARRFLLIGLILL
                     IGFFAGQRVLALYLDYLWFDSLHYVAVFTTTLKLKIGLFIGFALATLILIRTPFWLLE
                     RASHLERIQTSFEINGQPVAFSPRFPLRTTAWLVAGVMSMYFGSTMASDWSQYALFFH
                     QSPTSVTDPIFGRQIGYYLFTLPLQVQWAEWLWTLSGVVFVGTCLVASLVASQTSPVP
                     PAVLRGVSLSFGVFLAAWAWDVYLSRFTLLLADHTVLSGITYTDAHIRVPGLLAVTIA
                     MGMGTLLAFLNALGSGKLRLLVLGAGLPLVVYVVTGLLIPSYVTNFIVRPNELAKETP
                     FITHNIQATRAALKLNDIEEQTYEVEFSPEALSLQGDNLTLNNIQLWDWRALQATLKQ
                     IQEIRTYYDFPDIDIDRYVINGKLRHVLLAARELDINKLPVSSRNWINEKLIYTHGYG
                     ATMNPVTQFTPEGMPNLVLANMPVESKAPELKVTRPEIYYGELTNSTVYVKTKQKEFH
                     YPQGETNEATVYEGQGGIR"
     gene            complement(1722..2615)
                     /locus_tag="PKZ53_06530"
     CDS             complement(1722..2615)
                     /locus_tag="PKZ53_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNJ40128.1"
                     /translation="MASLGYELKQAREAKGVTLTQLAENTRIGIRFLQAIESDDFKSL
                     PGGLFNRSFIKAYARAVGIDEDEAVIAYQKQTGDGPANSDAQLNNIPTYTVPREEEIN
                     FVPYIIGAVVVVILFLGCWALYRHFNTPGQQTSQPTTAPPPGTPPPTAPTTVPPVLSG
                     GAPPPAPTPPTVAPTTTAPLVLELLAANDDCYVKVDPDGKIEKGVVLKMGQNQTFEAT
                     EQMLVRVGKVASVTAKLNGKEITLESPTGSREDFKISFKDGQPAIERIKRFIPKNKPA
                     ATGDATTGTPPATTGGTGNQQ"
     gene            complement(2833..>3727)
                     /locus_tag="PKZ53_06535"
     CDS             complement(2833..>3727)
                     /locus_tag="PKZ53_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNJ40129.1"
                     /translation="VTKNSMKILTIINPSAGQTKAQKSIKTLPGLYRRYNLESTICIS
                     QSAEHVQQLAAQAVQDTYDIVAVCGGDGTLHHAINGLQGSQTALGILPMGSGNDLAGA
                     LGIPTNLDAACQILKQGHHRGLDLADTGRRVYACIAGVGLDSEVGKRTRHRPAWIRGK
                     LIYPYAVLRSIFTFRPKFLRLIHDGGVFEGEILIAVVGNTRQYGGGMRIVPQADPEDG
                     FLDVCIVRPMSQWYLLKAFPSVFTGKHVSDPHVIVLRTQSVRLETHEPLEVFGDGEFL
                     EQTPSQIKVRRNALQVIAPGR"
BASE COUNT          904 a          894 c          989 g          940 t
ORIGIN      
        1 atccggattc cgccctggcc ttcatacacc gtggcttcgt tggtttcacc ttgcggatag
       61 tgaaattcct tttgtttggt tttgacatag accgtgctgt tggtcagttc gccatagtag
      121 atttcaggac gtgtgacttt gagttccggt gctttgcttt ccaccggcat attggccagg
      181 accaggttgg gcataccttc tggcgtaaac tgggtgaccg ggttcatcgt tgccccgtat
      241 ccgtgggtgt aaatcaattt ttcgttgatc cagttgcggc tgctaaccgg cagtttattg
      301 atgtcaagtt cacgggctgc cagcaggaca tggcgaagtt ttccattgat gacatagcgg
      361 tcaatgtcga tgtcaggaaa gtcatagtag gttcgaattt cctggatttg cttcagtgtt
      421 gcctgcaatg cccgccaatc ccagagttga atattgttga gggtgaggtt gtctccttga
      481 agggaaagag cttctgggga aaactcgacc tcataggttt gttcttcaat gtcattgagt
      541 tttaatgccg cccgtgtggc ttgaatattg tgggtaatga atggggtctc tttggccagt
      601 tcgttagggc gaacaataaa attggtgaca taggatggga tcaacagccc agtcacgaca
      661 tagaccacca gtggcaaccc ggcaccgagg accaggagcc gcagttttcc actccccagt
      721 gcattgagaa atgccagcag tgttcccatc cccatggcaa tggtgacagc cagtaagccc
      781 ggaacccgaa tatgggcatc ggtatacgtg atacctgaca ggacggtgtg atcagccagg
      841 agtaaggtaa accggctcag gtagacatcc caggcccagg cagccagaaa tacgccgaag
      901 ctcagcgata ccccgcgcaa tacggctggt ggaactggcg atgtttgaga tgccaccagg
      961 ctggccacca gacaggttcc aacaaaaacc acaccgctca gcgtccataa ccattctgcc
     1021 cactgaactt gtagcgggag agtgaataaa tagtagccaa tctgacggcc aaagattgga
     1081 tccgtgacac tggttggtga ctggtggaaa aacaacgcat actgtgacca gtccgaagcc
     1141 atggtggagc cgaagtacat actcatcacg ccagccacga gccaggcggt cgtccgtaag
     1201 gggaagcgtg gcgaaaatgc caccggttga ccgttgattt caaaggatgt ttggattcgc
     1261 tcaagatgtg atgcccgctc aagcagccag aatggtgttc ggatcaggat cagggttgcc
     1321 agtgcaaacc caatgaagag gccaattttc agttttaatg tggttgtaaa gactgcaaca
     1381 tagtgcagtg agtcaaacca cagataatcc agatacaggg ccagtacccg ctgaccagca
     1441 aaaaagccga tcagcagaat cagcccgatg agcaaaaatc gtcgagcctg tccttcaccc
     1501 tggtttactg cagttgctgg agagggaatg cgtggtggtt tacggcctgg ttcggcgggt
     1561 tcaataattt caacatcctt cataaaaccc tcatttccat cagaaatctg atgtgtgagc
     1621 aaaggtcgtg tgtggttgcc cagaaaattg tctcaaaaac aacaatggcg gactcatcgt
     1681 ccgccacaag actaacccgt caaccagaat ggtcaccagt tctattgttg gttcccagtg
     1741 ccaccagtgg ttgcaggagg tgttcccgtg gtggcatcgc cagtggcggc gggtttgttt
     1801 ttcggaataa accgcttgat gcgttcaatc gctggctgtc cgtccttgaa tgagatttta
     1861 aaatcttctc ggctcccggt tggtgattcg agcgtgattt ctttgccatt cagtttggcg
     1921 gtgaccgacg ccaccttccc cactcgtacc agcatttgtt cggtggcttc aaaggtttga
     1981 ttttggccca tttttaaaac cacccctttt tcaattttgc catcggggtc aaccttcaca
     2041 tagcaatcgt catttgccgc caaaagttcc agaaccagcg gtgccgttgt ggtaggggca
     2101 accgtgggtg gtgttggtgc gggtggtggg gcaccaccgc tcaataccgg aggcactgtg
     2161 gttggggccg ttggtggtgg tgttcctggt ggtggggctg tggttggttg tgaagtttgt
     2221 tgaccggggg tgttaaagtg ccgatacagt gcccagcacc ccagaaagag aatcacaacc
     2281 accacggcgc caataatata aggcacgaag ttgatttctt cttcgcgggg cacggtatag
     2341 gtcggaatat tgttgagctg agcatcagag tttgccggtc catcgccagt ttgtttttga
     2401 taggcgatta cggcttcatc ttcatcaatg ccgactgcac gcgcataggc tttgatgaac
     2461 gagcgattaa acaacccacc cggaagcgat ttaaagtcat cggattcaat cgcctgaaga
     2521 aatcgaatgc caattcgtgt gttttcggcc agttgcgtga gcgtaacacc tttcgcttca
     2581 cgggcctgtt ttagttcata acctaaggaa gccacgggac actcctcgaa aagatctcaa
     2641 tgagaaaaga taaaatacca gggaaaatga tggaagctga aatgttaact tgcccgccat
     2701 ggggtgtcaa attgagattt cgagggcgtt tcagctttca cctgtatgga cctggaaatg
     2761 ttctggaaga aaaagatcaa cttgaaagca tttgcatgat ggttgtgcgg cgccgggcaa
     2821 tgtagtacca ctttaccttc ctggtgcaat aacctgtaag gcattccggc ggaccttgat
     2881 ctgggatgga gtttgttcca gaaactctcc gtcaccaaaa acctcaagtg gttcatgcgt
     2941 ttccaggcga actgattggg tacgcaggac aatgacgtgg gggtctgaga cgtgttttcc
     3001 agtaaatact gaaggaaatg cctttaataa gtaccactgg ctcattggtc gaacgatgca
     3061 cacatccagg aacccatctt cagggtccgc ttgaggaaca atcctcatgc cgccgccata
     3121 ctgacgggta tttccaacca ctgcaatcaa gatttcacct tcaaagacgc cgccatcgtg
     3181 aatcaggcgt aaaaacttag ggcgaaaggt aaaaattgac ctcaatacgg cataggggta
     3241 aatcaatttt ccgcggatcc aggctggccg atgacgcgtc cgtttcccaa cctcagagtc
     3301 aagaccaact cccgcgatac aggcatatac acggcgacct gtatccgcca ggtccagccc
     3361 ccgatgatga ccttgtttga gaatttgaca ggccgcatca agatttgttg gaataccaag
     3421 ggcgccggcc aggtcattcc cactccccat aggtaaaatt cctaatgcgg tttgtgatcc
     3481 ctggagccca ttgatggcgt ggtggagcgt tccatctcca ccacacactg ccacaatatc
     3541 ataggtatct tgaactgcct gggcggcaag ttgttggaca tgctcagcgc tttgactgat
     3601 acaaattgtt gattccaggt tataccggcg ataaagaccc ggcagtgttt tgatactttt
     3661 ttgagctttt gtctggccgg ctgatgggtt gatgatggtt aaaattttca ttgaattctt
     3721 ggtgacc
//
LOCUS       DAOQDO010000324         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_17959_length_3722_cov_3.064903, whole genome shotgun sequence.
ACCESSION   DAOQDO010000324 DAOQDO010000000
VERSION     DAOQDO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17959_length_3722_cov_3.064903"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..936)
                     /locus_tag="PKZ53_06540"
     CDS             complement(226..936)
                     /locus_tag="PKZ53_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40130.1"
                     /translation="MINKQSRLAISMMGERPEKYDWVTGEILVERYGQSHPHFLLKQN
                     DRVLAKIDWPFARRGNYTSTDGSVDLDLVVSQMGRTLVAKDLMSHMSTLHVKRTMNRK
                     HSKLMICLSDSDCFLIKPQIDRTKPWRFSLHITKQFYSSELFDIEILTSQEKARRRRR
                     HDARVVAKIFINPIMRWEGRHFHHLMALVVGRIIFIGKHDKFHFDSVHNQYFSVEGAN
                     VVSQKRRNHHFQDRQFLL"
     gene            complement(1124..2158)
                     /locus_tag="PKZ53_06545"
     CDS             complement(1124..2158)
                     /locus_tag="PKZ53_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNJ40131.1"
                     /translation="MSFDRYSRHILFSGIGKAGQQRLLDSRVAILGCGALGCMQVESL
                     ARAGVGFLRIIDRDFIEFSNLQRQVLFDEADAEQQLPKAIAAKNHIHRINSEIQVEAV
                     VTDARAANIEELITDVDLVIDGTDNFETRFLLNDACVKQGRPWIYGAAVGAYGLTMTI
                     RPGQTPCLRCVLGENPPPGSSPTCDTAGIILPAIAMIASAQITEALKLLTGQVEKLRG
                     TLWQIDLWENTVRSTKLDGVREAGQCLCCVQGTYEYLDTATSQLTAMLCGRGAIQITP
                     ATQAKAKLDLQELATRLQPVGEVKANPFLVKCRIGAHELTIFSDARAIIKGTDDLLTA
                     RSLYAKYVGV"
     gene            2747..>3722
                     /locus_tag="PKZ53_06550"
     CDS             2747..>3722
                     /locus_tag="PKZ53_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ40132.1"
                     /translation="MPPQEAFPGTERFEVRRRLGGGAFGVVYEVFDRQRESTVALKLL
                     RQADSAPLYLFKQEFRNLANLVHQNLVTLYELQADGDQWFFTMELVEGIDFQRYVATQ
                     QSPIRADTTTILGLPTADSAPTNLPSEQVPTSILTDTGSSMSSDPTSDIQIFIRSGNN
                     HTGSSSPTVDSFGELISEAPTVDSLSGLIAENPTVDSFSEPISEAPTVDSLEGLSEAP
                     TVHSLEFTSETPTVDSSAFALNRTGMGKKTAPTQVTVTQLAYTFNEARLREGMRQLVA
                     GVNALHEVGKLHRDLKPSNVLVTPEGRVVILDFGLVAELKEYADPGNRE"
BASE COUNT          939 a          910 c          928 g          945 t
ORIGIN      
        1 tcatcaggta ccaaaactgg ggaacaagtc agtattttgg gcgaaatttg gggactgaag
       61 aatgaagaat gaagaaccca tctagtggtt agtgatgagt ggttagtggt tagaaatcaa
      121 tacttccgaa gaaaaaccaa gaaccaagca ccaggcacca agcaccaaga accaagcacc
      181 aagaattaac gactaaaagg attcttcatt cttcattgat ttggtttaga gcaggaattg
      241 ccggtcttga aaatgatggt tccgccgttt ttgtgaaacg acgttggcgc cctcgactga
      301 aaagtactgg ttatggactg agtcaaagtg aaatttatcg tgtttgccaa taaaaataat
      361 gcgtccaacc accagcgcca tcaaatggtg aaagtggcgg ccttcccacc gcatgatcgg
      421 gttgataaag atttttgcca ccacgcgggc gtcgtgtcgg cgacggcgac gggctttttc
      481 ctggctggtc aggatctcga tatcaaagag ttctgatgaa taaaattgct tggtgatgtg
      541 cagcgaaaag cgccagggtt tggtgcgatc aatctggggt ttaatcaaaa aacagtcgct
      601 atctgacagg caaatcatca acttagagtg tttccggttc attgtccgtt tgacgtgcaa
      661 cgttgacatg tgactcatca ggtccttggc aaccagtgtc cggcccatct gtgacacgac
      721 taaatccaga tctacggaac catcagttga ggtgtagtta ccccgtcgag caaaaggcca
      781 gtcaattttg gccagcaccc ggtcattttg ttttaaaaga aaatgcggat gggactgccc
      841 ataccgttcg accaggattt caccagtgac ccaatcatat ttttcagggc gttcgcccat
      901 catgctgatt gccagacgac tttgtttatt gatcattgga agtttcctcg gaaaaggagt
      961 tgagagttcc gccttcaggc ggtttttttg attgagagat gacgaggtct gcggttttca
     1021 gtcaggtagt tttcaggctg gatgcgtgtc tgaatgatag cgtgtatctg caccaatcaa
     1081 aaccaattgc tgaggcagca aatattgatt ggatgacaat gtttcacact ccgacatatt
     1141 tggcgtagag gctgcgtgcg gtcaagagat catcggttcc tttgataatg gcacgggcat
     1201 cagaaaaaat cgtcaattca tgcgctccga tgcgacattt caccaaaaaa ggattggctt
     1261 tgacctctcc gacaggttga agccgtgttg ccagttcctg caaatccagt tttgctttgg
     1321 cctgggtggc gggagtgatc tgaattgccc cacgtccgca caacatggcg gtgagctggc
     1381 tggtggcggt atcaaggtat tcatacgtgc cttgaacaca gcacaggcac tggcctgcct
     1441 ctcgtacccc atccagtttg gttgaccgaa ccgtgttttc ccacaggtca atttgccaga
     1501 gcgtgccgcg caatttttca acctgtccgg tgagcagctt gagtgcctct gtaatttggg
     1561 ctgaagcaat cattgcaatc gccggcaaaa taattccagc cgtgtcacag gtcggagacg
     1621 accccggcgg cggattctcc cccaaaacac agcgtaaaca gggagtttgg cctggtcgaa
     1681 tggtcatcgt taacccatag gccccgacgg ctgctccgta aatccagggc cgaccctgtt
     1741 tgacacaggc atcattgagc aaaaaccggg tttcaaagtt gtcagtccca tcaatgacca
     1801 gatccacatc ggtgatgagt tcttcgatat tggccgcacg cgcatcggtg accacggctt
     1861 caacctgaat ctctgagtta atccgatgaa tatgattttt agcagcgatt gcttttggaa
     1921 gctgttgttc agcatcggct tcgtcaaata acacctgccg ctgaaggtta gaaaattcaa
     1981 taaaatcccg gtcaatgatg cgcaaaaacc caacaccggc tctggccagc gattccacct
     2041 gcatacagcc aagcgctccg cagcccaaaa tggccacccg gctatcaagc aatcgttgct
     2101 gaccagcttt gccaatgcct gaaaacaaaa tgtgtcgtga atagcggtcg aaagacataa
     2161 ttagggttca gggttcaggg ttcagggttt gggaaaggac aaacaggaca aacaggacaa
     2221 aaaagacgaa aagaacagtg ctggtttggt gaggtaggtt ttttcgaaaa cccggaaccc
     2281 ggaaccttgt cattacccaa atcttttcag ttctccaaat agaatggccc aaattgcttt
     2341 ggtcagaact gaacttcatt cttcttttac cacagaggac acagaggcac acagaggcac
     2401 acagaggttt gagaaattga tcttcctctg tattcctctg tgtcctctgt gtcctctgtg
     2461 gtgaaatttt tcatttcagt cgtgagttgt gacgaatcaa aataatttgg gtaatgacaa
     2521 ggttctgggt tccgggcctt cgaagaaatt gtttttatgt ccttttcgtc ccgtatgtcc
     2581 tttttgtctt tttctcgaaa acccggaacc cggaactcgg aacccggaaa ccctctcaat
     2641 tcaccatcac ttgataagtg gttgaacatc gtggcacttc ccggcataat agccgggcct
     2701 gaaatcatgc tttcctgtac tccaccctac aaccctttag gtcacgatgc ccccacaaga
     2761 ggcttttcct ggaaccgagc gattcgaagt gcgccgcaga ctcggcggcg gcgcttttgg
     2821 cgtggtgtat gaagtcttcg accgccagcg tgagagtacc gttgccttga aattattgcg
     2881 tcaggctgat tccgctccgt tatatttgtt taaacaggaa tttcgcaacc ttgccaatct
     2941 ggttcatcag aatctggtga cgttgtatga gttacaggct gatggtgacc agtggttttt
     3001 caccatggaa ctggtcgaag gaattgattt ccaacgctat gttgccacgc aacaatcgcc
     3061 gatcagggct gataccacga ccatcctcgg gttgccaacg gcagattcgg ctccgaccaa
     3121 ccttccatca gaacaggtgc cgacgtcaat tttgactgat accggcagtt cgatgagttc
     3181 tgacccgacc agtgacatcc agatttttat tcgctcaggc aacaaccata ctggaagttc
     3241 atcgccgacg gttgactcat tcggcgaatt gatttccgaa gccccaacgg ttgattccct
     3301 ttccggcctg attgcggaaa atcccaccgt tgattcattc tccgagccga tatctgaggc
     3361 accgacggtt gattcgcttg aaggattgtc tgaagcccca actgttcatt cccttgagtt
     3421 tacctctgaa actccaaccg ttgactcctc cgcctttgcc ctgaatcgca ccgggatggg
     3481 aaaaaaaacg gcgccaaccc aggtcacggt gactcaactt gcctacacgt ttaacgaagc
     3541 gcggttgcga gaggggatgc gccagttggt ggcgggggtg aatgcgctgc acgaagttgg
     3601 aaaattgcat cgggacttaa aaccctcaaa cgtgctcgtc accccggaag gccgggtggt
     3661 gattctggac tttggcctgg tggctgaatt gaaagaatat gccgaccctg gaaaccggga
     3721 ga
//
LOCUS       DAOQDO010000325         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18089_length_3705_cov_2.299452, whole genome shotgun sequence.
ACCESSION   DAOQDO010000325 DAOQDO010000000
VERSION     DAOQDO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18089_length_3705_cov_2.299452"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKZ53_06555"
     CDS             complement(<1..639)
                     /locus_tag="PKZ53_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40133.1"
                     /translation="MHQHTRRVFIILFMTGIISTTYWLGPIESTSSDQKSQSAQSKTQ
                     AKSPPPVGAKSLPENQVPGRTSRRRSSVGGTVSSGVAGKPSTQAADTSNVPTPAIPVG
                     QSGEMIGVPWTGAAGIIETTEQMMIRERQAQLLPRKPRPLRKELEYERPKDLPQNPDA
                     PAVSQWPPVDESNSSQRPGQPGFTVQPLAPQTTSTSFTGATLADTGAFPPDTM"
     gene            1251..3581
                     /locus_tag="PKZ53_06560"
     CDS             1251..3581
                     /locus_tag="PKZ53_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ40134.1"
                     /translation="MLLEEPFTEIYKFGDFRLEVAERRLFRNGEPVLLTPKAFDVLVI
                     LVRNDGKLVTKQELLNQVWPNMVVEEANLANNISLLRKTLGSIPTDEPLIETVPRHGY
                     RFRGNATLVSQPLPPRPFMIERYTVMEVQTEEIETPTGESEVFPVIDLPPDSPDPRVS
                     EPGKTKDFIPASPAPKLIESPDQSGTTSGTKAGKGLRLYRWPLITLFGVILVSFGYFG
                     FHSSEQKTSTSLPHPRILPVTNQSGEKLNPSFSPDGKQIAYAWDKYETENFDIYITQI
                     GSLTPKQITDSPLKEFRPVWSPDGKYLAFLRGPENGLELIIKSLDTNSERSFGPVAGG
                     LAWSQDGQYLAVQDKISPESPTTIVFLEVATGKRQPVTQPVAPWNDMRPAFSPDGRQL
                     AFLRRQNNHGDIWVMPVTGGAPRQVTFDHRVTDSIAWMPDGKTLIFSSDRAGEFGVWQ
                     VSVNGGEPQSVSPISGQCDDIAVSPTQPHLVYHQRSISGNLWSLKRSSPAAEWSPPTI
                     AKAVPITTSTRMNDSPSFSPDGRRLVFSSNETGATEIWTCDADGGNPTRLTFFNQDIA
                     GSPHWSPDGKSIVFDGGINGVRQIFTISAQGGTPTVLTDASVPSYLPSWSSDGKWIYF
                     CSVPTGVRQIWKIPSTGGTPVQVTQGGGWECAESTDGRYLYYTKDRAVAGLWQLDLAT
                     NTESPIPELAEAGRYRYWAVSQDGIFFATPNASQTLIINFFDFKTRTIKEIMPIRGLI
                     LRGPSGLAISPDMTHLVFAQKDQFGSDLILVENFRW"
BASE COUNT          887 a          967 c          900 g          951 t
ORIGIN      
        1 catggtatct ggcggaaagg cgccggtatc ggccaacgtg gcacctgtaa atgacgtgct
       61 tgtggtttgc ggcgccaacg gctgaacagt aaaccctggt tgaccaggtc gttgcgatga
      121 attgctttca tccactggtg gccattggga aacggcgggc gcatccgggt tttgtggaag
      181 atctttcggg cgttcgtatt caagttcctt ccggagtgga cggggttttc tcggcaggag
      241 ctgggcctgt cgttcccgaa tcatcatttg ttctgtcgtc tcgatgattc cggcagcacc
      301 agtccagggg acgccgatca tctctccact ttgtccaact ggaatcgccg gcgttggcac
      361 atttgaggta tcagccgcct gggttgaggg ctttccagct accccgctgg aaactgttcc
      421 gcctacactg cttcgtcggc gtgaagttcg acctggtacc tggttttctg gcaaagattt
      481 tgcccccacc ggcggcgggc tctttgcctg agtctttgat tgagcagatt gagatttctg
      541 gtctgagctg gtgctttcaa ttggaccaag ccagtaggtc gtcgaaataa tgccagtcat
      601 gaacaggata atgaataccc gtcgggtgtg ttggtgcata gacgttcctc tattcagatt
      661 tgcgaaccat aaagaataga gggctgggtc attcacgctt cagcttgaag atcgagatac
      721 acaacaacct actcttcctg gcgactaagc aactgcggcg tttgattagt ggtagtttgg
      781 agatgggaaa tgggggcacg tagctttttt gagaagagcc aggattatag ttttgcccat
      841 aaaaaaacac catagtccag tgaaaaaaag ttttttccag gaattttttc gaacattttc
      901 cgtctaactt gttccgttaa ctggctcctt ccgaaatctg acatcaacca gtgtttaggg
      961 tggaaacctg ttaaaatgac aggtgccacg atgacaaata ataaaatgat gaaggctcaa
     1021 ctggtcacct gtcagcttgt cacccgttat gagctgggtg cgtgtgagca catctttcct
     1081 tcaccagctt tttctgctaa aaaagttagt tcggcatccc ttcgctttct cagggccaat
     1141 ccctcctgaa aacctttttt cgaacgatgg ctgcggcctt atccaaactg acctgtcaac
     1201 gtctttccca ttcccctcat ttttctcatt tttccatacg acacatcaaa gtgctgcttg
     1261 aagaaccatt tacagaaatt tataaattcg gtgattttcg gctggaagtg gctgaacgac
     1321 ggctctttcg aaatggagag ccagtcttgc tcacgcccaa agcctttgat gtactggtaa
     1381 tcctggttcg caacgatggg aaactggtca ccaaacagga gcttctcaac caggtttggc
     1441 caaacatggt ggttgaagaa gccaatctgg ccaataatat ttcactcctt cgcaaaaccc
     1501 tgggttcgat cccgactgat gagccattaa ttgaaaccgt tccacgtcac ggctatcggt
     1561 ttcgtggcaa tgccaccctg gtcagtcagc ctcttccacc tcgcccgttt atgatcgagc
     1621 gatacacggt catggaggtt caaacggaag aaatcgagac ccccacaggt gagtctgagg
     1681 tcttcccggt gattgacctg cctcccgatt cacctgatcc cagggtgagt gaacccggca
     1741 aaaccaaaga tttcattcca gccagtccgg cgccaaagtt gatcgaatct cctgaccaat
     1801 caggtacaac ttcaggcaca aaagctggaa aagggttgcg cctgtaccgg tggccgctga
     1861 ttaccttatt tggtgtcatc ctggttagct ttggctattt tggcttccac tcttctgagc
     1921 aaaaaacgtc aaccagcctc ccgcatccac gcattctccc cgtcaccaat cagtcaggtg
     1981 aaaagctcaa tccttcgttt tcgccggatg gtaaacaaat cgcctatgcc tgggataaat
     2041 atgagactga aaattttgat atttatatca cccagatcgg aagcctgaca cccaaacaaa
     2101 tcacagacag cccgctgaaa gaatttcgtc cagtctggtc accggacggc aaatatctgg
     2161 ctttcttacg gggtcctgaa aacgggttgg aactgattat taaatcactg gataccaatt
     2221 cggaacggtc atttggaccg gttgccggcg ggctcgcctg gtcacaggat ggtcaatatc
     2281 tggcggttca ggacaaaatt agccctgaat ctccaaccac tattgttttt ctcgaagtcg
     2341 cgactggaaa acgtcagccc gtaacccaac ccgtggcccc ctggaatgat atgcgtccag
     2401 cattttcacc cgatgggcgc caactggcgt ttctgcgtcg gcaaaataac catggggata
     2461 tttgggtgat gccagtcacg ggtggagccc caagacaagt gacttttgat catcgagtta
     2521 ccgatagcat tgcctggatg ccagatggga aaaccctcat cttttcttca gatcgggccg
     2581 gtgaatttgg tgtctggcag gtttcagtca atggtggtga accacaaagc gtatcaccga
     2641 tcagtggtca gtgcgacgac attgcggtat ctcccaccca acctcacctg gtttatcatc
     2701 aacgatcaat cagtgggaac ctgtggtccc tcaaacgttc cagtccagca gccgaatggt
     2761 caccacccac cattgccaaa gccgtcccca ttacaacgtc aacccgcatg aatgacagtc
     2821 cgagtttttc gccggatggc cgccggctgg tgttttcctc aaacgaaacg ggtgcgactg
     2881 aaatctggac ctgtgacgca gatggaggca acccgacccg actcacgttt ttcaaccagg
     2941 atattgccgg atcgccccac tggtcacccg atggcaaatc tattgtgttt gacggaggca
     3001 taaacggtgt tagacaaatt tttacaatca gtgcccaggg aggaacacca actgtcctga
     3061 ctgatgcctc cgttccaagc tatctgccat cctggtcaag cgacggtaaa tggatttatt
     3121 tttgttctgt accgactgga gttcgccaaa tctggaaaat cccctcaaca ggaggcacac
     3181 cagtacaggt cacgcaagga ggaggatggg aatgtgctga gtcaaccgat ggcaggtatc
     3241 tctattacac caaagatcga gccgtcgctg ggctctggca actggacctg gcgaccaata
     3301 cggaatctcc aattccagaa ctggcagagg ctggtcgcta tcggtactgg gcggtttcac
     3361 aggatgggat cttctttgcc actccgaatg cctcccaaac cttgattatc aacttctttg
     3421 atttcaaaac ccgtaccatc aaagaaatta tgccgattcg cggtcttatc ctgcgcggcc
     3481 catccggcct ggccatctcg ccagacatga cccacctggt ttttgcccaa aaagatcaat
     3541 tcggcagcga tttgatcctg gtcgagaact tccggtggtg attctcagtc agtcatcagg
     3601 atctggtaaa aacaaaaact tctgattgtt ctccacaatg attttggagc tgaagctgga
     3661 tctgtttgcc gggtttcacc agcttctctt tgactggaac cacca
//
LOCUS       DAOQDO010000326         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18138_length_3700_cov_2.989575, whole genome shotgun sequence.
ACCESSION   DAOQDO010000326 DAOQDO010000000
VERSION     DAOQDO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18138_length_3700_cov_2.989575"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1243
                     /locus_tag="PKZ53_06565"
     CDS             263..1243
                     /locus_tag="PKZ53_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNJ40135.1"
                     /translation="MTLFRSRNLSLFYQQLGQLYKSGVPVSQSLPLAAEHSGDTAIRE
                     ATERVYADVMGGKTLGSAVARQPQVFTELQAALIAVGEENGRLDSNLFQLSTMYEKQY
                     KNQLRFLLAMLYPSFLFLAALFVPKIPIWWNGSFGEYLSAVFWTAAPFLAIGVALYVG
                     FVLSQGVFIDMYDQVRLKIPVLGKSLHKMALARFSRCLATLYSAGIDMRRCLQLSTSS
                     FENTYLKRKSQIILGYLEEGFGLAKGFEAAGVFPKEYVHIIALGEQTGDLDRVLEQTA
                     RMYEEESEKVLKAVLFFFPIAMYVLVALYIASIVIEFYAGYYGNIGKMVP"
     gene            complement(1255..1806)
                     /locus_tag="PKZ53_06570"
     CDS             complement(1255..1806)
                     /locus_tag="PKZ53_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40136.1"
                     /translation="MRYMCVRFAFLSLLVCIPVLAQTQPSKPAPVAADSCETDPGFHK
                     LDFWLGNWDVFVKDQKVGTNQIEKILSGCAVTEYWADADGSQGKSLFYYLPATKRWRQ
                     VWVTEQANRTGGVKEKELIEELKDGGLRFQGEIMLPTGKTYLDRTTLTPLSKDKVRQV
                     IEFSMDGGKSWKPAFDAIYLRKD"
     gene            complement(1849..2277)
                     /gene="ruvX"
                     /locus_tag="PKZ53_06575"
     CDS             complement(1849..2277)
                     /gene="ruvX"
                     /locus_tag="PKZ53_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNJ40137.1"
                     /translation="MTQNQRTLAIDWGQKRTGFAICDELGMTVRPLETVRGPKEAVLQ
                     RVAELVSDYDVQRIVVGVPLGAEGQIRGAAERILALVEELKGVVTCPIHTWNERLTSY
                     AADEWMRERGYSARERQQRSDEIAAAILLEDFLASRPFST"
     gene            complement(2433..2726)
                     /locus_tag="PKZ53_06580"
     CDS             complement(2433..2726)
                     /locus_tag="PKZ53_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40138.1"
                     /translation="MTFEEMERTMEFILNQQAKFSVDIELLKESIVEMKAQADLDRAI
                     IKDAIAEMRDGMTTMLGIAERMEKTTSNLVMAQTGLSQRVTRLEDRVDHLEES"
     gene            complement(2806..>3700)
                     /locus_tag="PKZ53_06585"
     CDS             complement(2806..>3700)
                     /locus_tag="PKZ53_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40139.1"
                     /translation="KSNDPFANLKPGQVIVSRRNANQTMRVIAPGEKDKFLNICRENF
                     ENPDYTPPADLIEWMKPYEEQKFSFVAISAVADLVDQNSITDEQVLMTPFRITFPAEK
                     PFVPMIRPAMAVPNGWRPYNVFRYFVVMPERADVRVGDQPFFDQSHLMYANRVSREEW
                     NTKIWPGLRMTTVQPTEPAPVPPKPDATPKTTVPAPAKPEPKPEAKPATPPPAASSVP
                     SPEEAPVGNDLFLTAYVRADQQLRPITQDGTLVPAATQTTFKLPTKVEFDDRDVSFPV
                     EGPLLVIGGIVLYLRQRRQAN"
BASE COUNT          921 a          931 c          888 g          960 t
ORIGIN      
        1 gcaccggtct caattcagcc accgctacgc ggcgaagaaa tgtcaacaga acctaagcac
       61 caagtaccag gcaccaagta ccaggcacca agtaccaggc accaagtacc aagtaccaag
      121 taccaagcac caagtaccaa acaccaggta ccaggtacca agtaccaagt accaagcacc
      181 aagcaccagg taccaagtac caagtaccaa gcaccagata ctaagcacca aacccagtct
      241 attctttttc ttcaggtaca gtatgactct ttttcgaagt cgcaatcttt ctctgttcta
      301 tcaacaactt ggacagcttt acaagtcagg cgtccctgtc agccagagcc tgcctctggc
      361 ggcagagcat agtggggaca ccgctattcg cgaagccacc gagcgggttt atgccgatgt
      421 gatgggggga aagacgcttg ggtcagccgt cgccagacaa ccacaggtgt ttaccgaact
      481 gcaggcggca ttgattgcag tcggcgaaga aaatggccgt cttgattcca atttgttcca
      541 gctttcgacg atgtatgaga aacagtacaa aaaccagctc cggtttttgc tggctatgct
      601 ctacccgagt tttttatttc tggcagccct ttttgtcccg aaaatcccaa tctggtggaa
      661 tggatcgttt ggtgaatacc tgagcgccgt tttttggact gcagcaccat ttctggcaat
      721 tggcgtggca ctctatgtgg gatttgtctt atcgcagggt gtctttattg atatgtatga
      781 ccaggtacgg ctcaaaattc cagtattggg taagagcctg cataaaatgg cgttagcccg
      841 atttagccgc tgcctggcaa cgctctatag tgctggaatt gatatgcggc gctgcctgca
      901 gctttcaacc agctcgtttg aaaataccta cttgaaacgc aaaagccaga ttattctcgg
      961 ctacctcgaa gaaggctttg gactggccaa agggtttgaa gccgcgggtg tatttccgaa
     1021 agagtatgtg cacatcattg ccctggggga acaaaccggc gatttggacc gggtgctgga
     1081 acaaaccgcc cgaatgtatg aagaagaatc ggaaaaagtt ctgaaagcgg tgctcttctt
     1141 tttccctatt gccatgtatg tgctggtggc actctatatt gcgagtatcg tgattgagtt
     1201 ttacgcaggg tattatggca acattgggaa aatggtacca taacctgatt gagttcaatc
     1261 ttttctgaga tatatcgcat caaaggcggg cttccaggac ttaccgccat ccattgaaaa
     1321 ttcaatcact tgccgtactt tgtcttttga gagtggcgtc aacgtagttc gatccagata
     1381 ggttttgccg gttggaagca tgatttcacc ctgaaatcgc aacccaccat ctttgagttc
     1441 ttcgatcaac tctttttctt tgacgccgcc agtacgattg gcctgttccg tcacccacac
     1501 ctgtcgccag cgttttgtcg ccggaagata gtaaaacaaa cttttgccct gactgccgtc
     1561 agcatctgcc caatactctg tcaccgcaca accactgaga attttttcaa tttgattggt
     1621 accaactttc tggtctttga caaacacgtc ccaattccca agccagaaat caagtttgtg
     1681 aaatccaggg tcagtttcgc aggaatcagc cgccaccggc gctggttttg atggttgggt
     1741 ttgagccagt accggaatac acaccagcag actcaaaaac gcaaatcgga cacacatgta
     1801 tcgcatacaa atcaacaggt tccatttcaa gaagatgtgc gccgggagtt atgtggaaaa
     1861 cggacgggaa gccaaaaaat cttcaagcag aatggctgcc gcaatttcat ctgagcgttg
     1921 ctggcgttca cgggctgaat agccacgctc gcgcatccac tcgtcagcag catatgaggt
     1981 taatcgttca ttccaggtgt gaatgggaca ggtcaccact cctttgagtt cttcgaccag
     2041 agccaggatt cgctcagccg ctccacgaat ttgcccttca gcaccaagcg gcaccccaac
     2101 cacaattcgc tggacatcat agtctgaaac cagttctgct accctctgca aaacggcttc
     2161 ttttggaccg cgaaccgttt caagcggacg caccgtcatt cccagttcat cacagatggc
     2221 aaaaccggtt cgtttttgtc cccaatcaat ggctaaggtt cgctggtttt gagtcataag
     2281 agccgggctg agggctgagg gtatagggct aaaggctaat ggaactacaa ttttatcaat
     2341 aatttaaccc tttgttaagg tagcaggatc attccaaaaa tgtatccccc tagcccttag
     2401 ccccacaccc ttaaccctaa atagtattgg aatcaggatt cttcgaggtg atcaacccga
     2461 tcttcaagcc gggttacacg ttgagaaagt ccggtttggg ccattacaag attgctggtc
     2521 gttttttcca tccgctcggc aatcccaagc atggtggtca tgccgtctct catttcagcg
     2581 atagcatcct ttataattgc ccggtccaga tcagcctgag ccttcatttc aacaattgat
     2641 tctttcagta actcaatgtc cacactgaat ttggcctgtt gattgaggat aaactccatc
     2701 gtgcgttcca tttcttcaaa tgtcatttga tgcctcatca ataaagtcgt gtttgaagga
     2761 actcagtggc tccgtgttac tgatgcttcc ttcgcttaca gtttatcaat tggcctgccg
     2821 tcgctgacgc aggtacagga caataccgcc aatcaccaac aatgggcctt caactggaaa
     2881 actgacatcc cggtcgtcaa attccacttt ggtgggcaat ttgaatgttg tctgagtggc
     2941 agccgggaca agtgtcccat cctgggtaat cggtctaagt tgctgatcag cacgaacata
     3001 cgccgtgaga aacaaatcat ttccaaccgg agcttcttca ggcgatggga cagacgatgc
     3061 agcaggtggc ggtgtggctg gtttggcttc gggctttggc tcgggtttcg ctggagctgg
     3121 cacagttgtt ttgggcgtgg catcaggttt tggcggtacc ggggctggtt cggttggctg
     3181 aacagttgtc atccgcaatc caggccagat tttggtattc cactcttcgc gcgagacccg
     3241 gttggcatac atcaaatgag actggtcaaa aaagggctgg tctccgacac gtacgtcagc
     3301 ccgctcgggc atgaccacaa aataccgaaa cacattgtag ggccgccatc cattggggac
     3361 agccatggct ggtcggatca ttggaacgaa tggtttttct gccgggaagg tgatccgaaa
     3421 aggggtcatc aagacctgtt catccgtgat tgagttttga tccaccagat cggcaaccgc
     3481 tgaaatcgca acaaaggaaa atttttgttc ttcgtaaggt ttcatccact cgatcaagtc
     3541 agccggaggt gtgtagtcag ggttttcaaa gttttcacgg cagatattaa ggaatttgtc
     3601 tttttcaccc ggagcaataa cccgcattgt ctgattggca ttgcgccgtg acacgatcac
     3661 ctgtccgggt ttgagattgg caaacggatc atttgacttt
//
LOCUS       DAOQDO010000327         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18156_length_3698_cov_2.990393, whole genome shotgun sequence.
ACCESSION   DAOQDO010000327 DAOQDO010000000
VERSION     DAOQDO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18156_length_3698_cov_2.990393"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1038
                     /locus_tag="PKZ53_06590"
     CDS             82..1038
                     /locus_tag="PKZ53_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40140.1"
                     /translation="MSRYPFEPLDFSNLSTYPLAERKSKVSVEDLARPIESGSSLREF
                     FDRLPNILAVQNLKAVAQAVRQARQRGKTVLVGLGGHVIKCGLAPVLIDLMNQGFING
                     FALNGSGVVHDFEIALIGSTSEDVDATLGSGAFGGAEETGRFINQAYSEALRDSIGGG
                     EAIGRLLIEMNPRFADRSLLCSAYQHSIPVTVHVAVGTDITHIHPTADGSAIGAASYH
                     DFRLFCSVVKGLDDGGVYLNLGSAVILPEVFLKAVTTLRNLGHQLEGFTTVNFDFLQH
                     YRPLTNVVRRPVAGGSGRGFALTGHHELMIPLLAAAIVEGWE"
     gene            1295..>3698
                     /gene="dnaE"
                     /locus_tag="PKZ53_06595"
     CDS             1295..>3698
                     /gene="dnaE"
                     /locus_tag="PKZ53_06595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNJ40141.1"
                     /translation="MSRKEFVHLHLHSDFSLLDGAIQHDALAEHASKLGFSAMAATDH
                     GNMFGAMSFYNSMKKHNVRPIIGMEAYVARGSMHERGTNTEGEKGTNHLILLAKNQTG
                     YENLVILTSLAYTKGYYYKPRMDKELLSKHSEGLVALSACMSGVPASLILRDKMDRAV
                     CEVGEYQEIFGKGNYFLELQCHDGFEEQQSRVNAGLTEIARKLDVPLVVTNDAHFLTR
                     DDFRAHQALLCLQTGKTIDQATMHYSPTHYVRSAEEMWHLFENQNVEALRNTMKIAEM
                     CQFGFPKAKMHLPEYPVPEGFTIDSYFEKVAREGLESRLHELAPLHARGELKYSVEQY
                     RARLDHEIATIKKMGFCGYFLIVWDFIRYARDNGIPVGPGRGSAAGAIVAYAMRITDI
                     DPLQFELLFERFLNPERVSMPDIDVDFCVRGRGQVIDYVGNFYGRENVSQIVTFGTMA
                     SRAAIKDVGRVLDIPYAEVEKIAKMIPPPQRGRNVPIGEALKTVPELKTAYEKDTRIR
                     EMLNLAQRLEGCSRHSSIHAAGVVISPRPVYELVPVFKTKAKDKERGEIDVLATQYNM
                     NDLEKAGMLKMDFLGLTTLTIINDCLENIQREQGQAPNLNEIPLDDPAALRLFADGET
                     EAIFQFEGDGIKEITRRLKPESLEDIIALNALYRPGPLDSGMVDDYIERRHGRKKVRY
                     DFPELKDILGNTFGVCVAGDTLVLDAHSGKRVRIDELESQIGTFYVQGVDQNLNSQIG
                     QVTHFFDNGLREVVELKLRNGSSVKVTQDHQVLTETGWRQVKDLQVNDFIATPRRLTV
                     ANE"
BASE COUNT          933 a          940 c          935 g          890 t
ORIGIN      
        1 aaaaaagaag cgtgattaac ggtttctcgc tatcccttat tccctatccc ctatccccca
       61 tcccctatcc cctatttttt tatgtcacgt tatccatttg aaccactcga cttttccaac
      121 ctttccacct atccactcgc tgaacgcaaa agcaaagtct cggttgaaga cctggccaga
      181 ccgattgagt caggcagctc gcttcgggaa ttttttgacc ggttgccgaa tattctggcc
      241 gttcagaact tgaaagccgt cgctcaggcc gttcggcaag cacgtcagag gggaaaaact
      301 gtcctggttg gactcggcgg gcatgtcatc aagtgtgggc tggcaccggt gctaattgac
      361 ctgatgaatc aaggatttat caacggattt gccctcaatg gctctggcgt agtccatgat
      421 tttgaaattg ccctgattgg ttcgacgtct gaagacgtgg acgccacgct tggttcaggt
      481 gcatttggtg gcgctgaaga aaccggacgc tttatcaatc aggcatacag tgaagccctg
      541 cgtgattcaa tcggcggtgg tgaagcgatt gggcgactct tgattgagat gaacccacgc
      601 tttgctgacc gttccctgct ttgttcagcc taccagcaca gcattccagt gacagtccat
      661 gtagctgtcg gaactgatat tacgcatatc catccaacgg ctgatggatc agcgattggc
      721 gccgcttcat atcacgactt tcgactgttt tgctcagtgg tgaaaggtct ggatgatggc
      781 ggggtttatt tgaatcttgg ctcagccgtt attttgccgg aagtttttct gaaggcggtt
      841 accaccttgc gcaacctggg tcaccagttg gaaggcttta ccacagtcaa ttttgatttt
      901 ctccaacact accgaccgct aaccaatgtc gttcggcgac ccgttgccgg agggtcagga
      961 cgcgggtttg ccctgactgg tcaccacgaa ttgatgattc cactcctggc agcagcaatt
     1021 gtggaaggct gggaatagtt taggggtcag gggtcagggg ttggaaaaag acgaaaagga
     1081 cataagggac tcaagggaca taaattcgaa gacccggaac ccggaaactg gaaaccggaa
     1141 ccgtgagttc tctctcaggc ttcaacaggc tataattccc tgaccaacga cagcaactca
     1201 gacgatctaa cctgaaatca atcagttacg agtagatcac actctcaccg tttctactcg
     1261 ctactcacga ttcgctacac actctaaatt acctatgtcc cgcaaagaat ttgttcacct
     1321 gcatttgcac tctgacttca gccttctcga tggtgcaatt cagcacgatg ctctggctga
     1381 acacgcctcc aaacttggat tttcggcaat ggccgctact gatcacggca atatgtttgg
     1441 agcgatgtcc ttttacaact ccatgaaaaa acacaatgtc cgccccatca tcggcatgga
     1501 agcctatgtg gcccgtggtt cgatgcatga gcgggggaca aacactgaag gagaaaaagg
     1561 gaccaatcac ctgattctgc tggccaaaaa tcagaccgga tatgaaaacc tggtgatcct
     1621 gacgtcattg gcctacacca aagggtatta ctacaaaccc cgcatggata aggagttact
     1681 gagcaagcac agcgaaggac tggtagccct gtcagcctgt atgtccggcg ttccagcctc
     1741 gcttattttg cgcgacaaaa tggatcgggc ggtgtgtgag gttggcgagt atcaggaaat
     1801 tttcggcaaa ggaaattatt ttcttgaact tcaatgccat gacggcttcg aagaacagca
     1861 atcacgcgtc aatgccgggc tcactgaaat tgcccggaaa cttgatgtgc cgctggtggt
     1921 aaccaacgat gcccattttc taacccgcga tgattttcgt gctcatcagg cactgctgtg
     1981 tctgcaaacc ggcaagacga ttgatcaggc cacgatgcac tatagcccga cgcactatgt
     2041 ccgaagcgct gaggaaatgt ggcatctctt tgagaatcaa aatgttgagg cgcttcgcaa
     2101 cacgatgaag attgcggaga tgtgtcagtt tgggtttcca aaagccaaaa tgcacctgcc
     2161 ggaatatcca gtccccgaag gcttcacgat tgacagctac tttgaaaaag tcgcccgtga
     2221 aggacttgaa agccggctcc acgaattagc tccacttcat gcccgcggag agttgaagta
     2281 cagtgtcgaa caatatcggg cacggctcga ccatgaaatc gcgaccatca agaagatggg
     2341 cttttgcgga tactttctca tcgtctggga ttttattcga tatgcacgag ataatggcat
     2401 ccccgtaggt ccgggccgtg gtagtgctgc gggcgcgata gtggcgtacg caatgcgcat
     2461 cacggatatt gacccgctcc agtttgagtt gctgttcgaa cgctttctta atccggaacg
     2521 cgtttcgatg cccgatattg acgtggattt ttgtgtccgt ggacgcggtc aggtgattga
     2581 ttatgtcggc aatttctatg ggcgggaaaa cgtctcgcaa atcgtcacct ttgggacaat
     2641 ggcctcgcgg gcggcgatta aagacgtcgg gcgggtgctc gatattccct acgccgaagt
     2701 cgaaaaaatc gccaaaatga ttccgccgcc acagcgcgga cgcaacgttc caattggcga
     2761 agccttaaaa accgttccag aactcaagac ggcctatgaa aaagacacgc ggattcgcga
     2821 aatgctcaac ctggcccagc gtctggaagg gtgttcgcgg cattcgtcca ttcacgccgc
     2881 cggagtggtg atctcgccac gacctgtcta tgaactggtc cccgtcttca aaaccaaggc
     2941 gaaagataaa gaacgcggtg aaattgacgt cctggccacc cagtacaaca tgaacgacct
     3001 cgaaaaggcc ggcatgttaa agatggactt tctgggactg accacgctca ccatcatcaa
     3061 tgactgcctg gaaaacattc agcgcgaaca gggccaggca cccaacctca acgaaattcc
     3121 gctggatgat ccagcagcct tgcgtctgtt tgccgatggc gaaactgaag cgattttcca
     3181 gtttgaaggc gatggaatca aagaaatcac ccggcgactg aaacctgaaa gcctggaaga
     3241 cattattgcc ctcaatgccc tctaccgacc cggcccactt gattccggca tggtggatga
     3301 ttatatcgaa cgccgtcatg gccggaaaaa agtccgctat gactttcccg aactcaaaga
     3361 tattttggga aatacctttg gtgtgtgtgt ggctggggat acgctggttc tggatgctca
     3421 ctctggaaaa cgagttcgca ttgatgaact ggagtcccaa atcggcactt tttatgttca
     3481 aggtgttgat cagaatctca attctcaaat tgggcaagtc acacatttct ttgacaatgg
     3541 attgcgtgaa gttgtcgaat tgaaattacg caatggctca agcgtcaaag taacccagga
     3601 ccatcaagtg ctgacagaaa ctggctggcg tcaggtaaaa gatctacaag taaatgactt
     3661 tattgccacc ccgcgtcgcc tgacagtggc caatgagc
//
LOCUS       DAOQDO010000328         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18168_length_3697_cov_2.410489, whole genome shotgun sequence.
ACCESSION   DAOQDO010000328 DAOQDO010000000
VERSION     DAOQDO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18168_length_3697_cov_2.410489"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..622
                     /locus_tag="PKZ53_06600"
     CDS             128..622
                     /locus_tag="PKZ53_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40142.1"
                     /translation="MKTLLILAFSMLAIVSSGYARQTAEIEIGPNIPTVKLCDLIRKP
                     QLYGRQMVRVQGIYRYGFEWSELYCLECLTEGSVWVEFDVSLKSRTKKKLIKRISGNG
                     DLGRTVNVVFVGQYFTGHGYGHMSSYSAELKVYAIEKAEILLNDSPIPAYLPPSVLRR
                     AKCQ"
     gene            complement(641..1285)
                     /locus_tag="PKZ53_06605"
     CDS             complement(641..1285)
                     /locus_tag="PKZ53_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40143.1"
                     /translation="MTMTMASKGRLVTVPALITLGVTLLRLIGELQNWSPVFFSREPG
                     GLGAIVGIIWLAPLSGIYFALKLLKQGDGPTTSQRAVGHAVLGFGLFIAFGLAMLFFW
                     PPYQAQVIGGAVVALGIVLLQLRGWPELGQVMLLYAFTARLPVVIVMLFTILGSWGTH
                     YDAFPSGFPLVNLWERWLWGGLVVQLTLWVGNTVFLGALCGSVAVAVRLWHQKR"
     gene            complement(1348..2238)
                     /locus_tag="PKZ53_06610"
     CDS             complement(1348..2238)
                     /locus_tag="PKZ53_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNJ40144.1"
                     /translation="MILALMVGNSCQTVPPPVVSTPVDIWLGGDVHLGKGNTNVLAPL
                     KSITHNATGIINLEGAVAETSSLREGIVLLNAPHRLAILKEAGVAVAGVANNHAQDAG
                     DQSLSHTIEQVRMAGLLPVGEMAGTAVFERERMRLVVSAHDLTGGVPPRLAEELRRAR
                     AQGDILIATFHVTGPPSYLPSRELKQAVDIALSAGAQVIAAHGSHLPGPIERRGPAVI
                     LWGLGNLVFDCDCTAEKDALVARITFDQGQVIKAQVFPIEAGLNGAPARLSANPAGMF
                     ELLTAIGSSPFQSDTDQADF"
     gene            complement(2274..>3697)
                     /locus_tag="PKZ53_06615"
     CDS             complement(2274..>3697)
                     /locus_tag="PKZ53_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ40145.1"
                     /translation="RRKFLASVSASTLVSGLFGSVTDVAAASQGKARTDETVVIIGAG
                     AAGLGAARKLKLAGVKVIVLEARDRIGGRVWTDRSLKNIPLDLGASWIHGIEGNPLTA
                     LVRQYEIETVSTDYDNVALYDAQGTEFTDRQQDQLDTRYSRIMRRVRRQGERAQQQGR
                     PDASLQSAISAAIAENSWTAEERLQFDYLINTNLEHEFAADAGELSWFSWDEGEEIVG
                     GDVLFPGGYGQIFSRLAEGLDIRFGQMVQKIEYSESQVTVVTRQGSIMAQRLIVTVPL
                     GVLKRGSIQFSPALPERKQAAIQRLGMGLLNKVFLHFPRVFWDRDSDLLGYIGPRKGE
                     WAEWYNFFKFTGEPVLLGFNAATYARRLEVLSNEAIVADAMTALRRMYGNSIPAPDGS
                     TVTRWASDPLSFGSYSFLAPGSSASDRDALAEPVSNRLFFAGEATFRDYPATVHGALL
                     SGEREAARILSLAKPASRVLLSR"
BASE COUNT          971 a         1027 c          888 g          811 t
ORIGIN      
        1 tgcttcgtga ccagaacgcc ctcacccgat ggcagacgat tttgcacgcc atgtaataca
       61 ggttggagtg ctgtgactta tcatagcttt ggtaggttca acctccagaa aaagaaagaa
      121 atttgacatg aaaacgctgc tgattttggc cttttccatg ctcgcaattg tgagttcagg
      181 atacgcccga caaaccgcag aaattgaaat tggtccaaac attccaacgg tcaaattatg
      241 cgacctcatc cgcaaaccac aactctatgg gcgccagatg gttcgggttc aaggaatcta
      301 caggtatgga tttgaatggt cagagcttta ttgtttggag tgtctgacag aaggaagtgt
      361 ttgggtagaa tttgatgtct cactcaaatc tcggacaaag aaaaaactga tcaaacggat
      421 aagcgggaac ggagatcttg gccgaacagt caatgttgtt tttgtcggcc agtatttcac
      481 tgggcacgga tatggtcata tgagcagcta ttcagcagaa ctgaaagttt atgccatcga
      541 aaaggcagaa attcttctca atgacagtcc aattccagca tatcttcctc cctcggttct
      601 tcgtcgagca aaatgccagt aaccccaatt cttcaatagc tcaccgcttt tgatgccaca
      661 gcctgacagc gacggccacg ctcccgcaca gtgccccaag aaacaccgta tttccaaccc
      721 acagtgtcag ttgcaccaca agcccgcccc acagccatct ttcccacaga tttaccagag
      781 gaaatcccga aggaaacgca tcatagtgtg tgccccagga gccaaggatg gtaaataaca
      841 tgacaatcac cactggcagc cgagccgtaa aggcatagag gagcattacc tgaccgagtt
      901 ccggccatcc tctaagctgt aacaggacaa tgcccagtgc cacgacagcc ccgccgatga
      961 cctgggcctg atagggtggc caaaaaaata acatggcaag tccaaaagcg ataaacaggc
     1021 caaagcccaa aaccgcatgg ccaactgctc tttgagatgt cgttggaccg tcaccttgct
     1081 tcaacaattt gagcgcaaaa taaattcccg aaagcggcgc cagccagatg attcccacaa
     1141 ttgcgccaag accaccgggt tcccggctaa agaagactgg agaccagttt tgcaattccc
     1201 caatcagacg caggagcgtc acaccaagcg ttatcagtgc cggtactgtg acaagccgcc
     1261 cttttgatgc cattgtcatc gtcatttttt ggtctcctgg gtatggaaat ctccctggtt
     1321 tatcaacttt cagttgccgt ttcttattca gaaatcagcc tgatctgtat cagattgaaa
     1381 cggtgagctc ccaatcgcgg tcagcaactc aaacattcct gctggatttg ccgagaggcg
     1441 ggcgggtgct ccgttgagtc cagcttcaat gggaaacacc tgtgctttta taacttgccc
     1501 ttgatcgaaa gtgattctgg cgacgagagc gtctttttca gcagtgcagt cacaatcgaa
     1561 gaccagattc ccaagtcccc acaaaataac cgcaggccca cgtcgttcaa ttggaccggg
     1621 taaatgactg ccatgagccg cgattacctg agcaccagca ctcaaagcaa tgtcaacggc
     1681 ttgttttaac tccctggatg gcaaatagga tggcggcccc gtgacatgaa acgtggcgat
     1741 caaaatatca ccctgggccc gggcacgccg gagttcttcg gccagtcgcg gaggaacacc
     1801 accggttaaa tcgtgcgcgg agacgacaag cctcattcgc tcacgttcaa acacggcggt
     1861 tccggccatc tctccaactg gcaacaaacc ggccattcga acctgctcaa ttgtatgtga
     1921 caaactctgg tcacccgcat cctgggcgtg attattggcc acaccagcca cggcgacgcc
     1981 agcttccttc aaaatcgcca gtcggtgtgg cgcattgagc aacacgattc cttcccggag
     2041 tgatgatgtt tcagccacag cgccttcgag gttaatgatt ccagttgcat tgtgcgtgat
     2101 tgatttgagt ggcgcgagga cgttggtgtt tcccttcccc agatgaacgt ctccgccaag
     2161 ccagatgtca accggtgttg acacaactgg cggaggcacc gtttggcagc tattcccgac
     2221 cataagcgcc aggatcatcc acaaccaaag aaaacaccat ggtgttcttt tcattagcgg
     2281 gacaacaaaa cacgtgaagc tggtttggca agtgaaagga tgcgtgccgc ttctcgttcg
     2341 ccagaaagca gcgcaccgtg aacggtggcc gggtagtccc gaaatgttgc ttcgcccgca
     2401 aaaaagagac gattggaaac cggttcggcc aacgcatcac ggtcagatgc cgaacttcct
     2461 ggggcgagaa atgaatagga gccaaaactc agcgggtctg aagcccagcg ggtcaccgtc
     2521 gaaccatccg gcgccgggat tgaattgcca tacattcgcc gcagggcagt catggcatca
     2581 gctacaattg cttcatttga aagtacttcc agccgacgag cataggtggc ggcattaaag
     2641 ccaagcaaaa cgggttcacc agtgaattta aaaaagttat accactcagc ccactcgcct
     2701 ttgcgcggtc caatatagcc aagcaggtca ctgtcacgat cccaaaacac acgtggaaaa
     2761 tgcaaaaaga ctttattgag cagccccatt ccaagtcgtt gaatggctgc ctgcttacgt
     2821 tccggcaagg cgggtgaaaa ctggatgctc ccgcgtttga gaacaccaag cggaacggtc
     2881 acgatcaagc gttgcgccat aattgacccc tgacgggtga cgacggtgac ctgcgattca
     2941 gaatattcaa ttttctgcac catctgccca aatcgaatat ccagtccttc agccagacgc
     3001 gaaaaaatct gaccgtagcc gccaggaaac aacacatcgc caccgacaat ttcttcgcct
     3061 tcatcccagg aaaaccagga taattcaccg gcatccgccg caaattcgtg ttcaagattg
     3121 gtgttgatca ggtaatcaaa ctggagtcgt tcttctgcgg tccaggagtt ttcagcaatc
     3181 gcagccgaaa tcgcactttg cagcgacgca tcgggccgac cctgttgctg agcacgttcc
     3241 ccttgacgcc tcacccgacg cataatgcgc gaataccgtg tatcaagttg atcttgttga
     3301 cggtcggtaa actctgtgcc ttgagcatca tacagagcca cattgtcata gtcggtcgaa
     3361 accgtctcaa tttcatactg gcgtaccagt gccgtgaggg ggttcccctc aattccgtga
     3421 atccacgagg cgcccaaatc cagcggaatg tttttcagtg accgatctgt ccagacacga
     3481 ccgccaatcc ggtcgcgagc ttcgagcaca atcactttta ctccggcaag tttcaattta
     3541 cgtgccgcgc ccaacccggc agccccagcg ccaatgatga caacagtttc atctgtccgg
     3601 gcttttccct gggaagcagc cgccacatcg gtcacacttc caaacagccc tgaaaccagg
     3661 gttgaagcag acaccgacgc aagaaatttt cggcggg
//
LOCUS       DAOQDO010000329         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18169_length_3697_cov_2.384404, whole genome shotgun sequence.
ACCESSION   DAOQDO010000329 DAOQDO010000000
VERSION     DAOQDO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18169_length_3697_cov_2.384404"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKZ53_06620"
     CDS             complement(<1..549)
                     /locus_tag="PKZ53_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ40146.1"
                     /translation="MNRRKVNLSTFVHLIALSALFVGTPLTALSDMPKIPAEQRLSAL
                     SGTVKDTIGLPLVGAVVSVLSGQDFRQVTTTSQTDARGWFQIPDLPPGLYCLKISATG
                     FQPLLGKPIEIGTNHQPESNFVLQRAATVLKSQSIDPVKYQSRRNRTVFQLDDDENPD
                     MLLAQEIHFQPQFHGSTTLMSQS"
     gene            complement(643..1689)
                     /gene="glpX"
                     /locus_tag="PKZ53_06625"
     CDS             complement(643..1689)
                     /gene="glpX"
                     /locus_tag="PKZ53_06625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNJ40147.1"
                     /translation="MKPSQSLPPSLSLEFLRVVELAAIDSSRTMGQGKRKFSDHVAVE
                     AMRDALRTVPINGRIVIGEGERDKAPMLYIGERVGIGAELPDEGNPYPAVDIAVDPLE
                     GTNLCATGSPNAISVLATAERGGLLNAPDMYMNKLVVGPAARGCLRIDAPVKENLKIL
                     ARRLGRTIEDHVVAVLDRPRHARLIQDIREAGARIQLISDGDLSAGISAAVGGSGVHA
                     VMGIGGAPEGVLTAAAMKCLNGEIQARFVLKDQLHQEDHSKVTDDSETLDRMKSMGLS
                     DPTKVFFTDDLAPGRHILFLMTGVTDGKVLRGVRFFGTGKRTASLIMKTEPHQVRFVD
                     TVHIEGGPDTVIRF"
     gene            complement(1751..2035)
                     /locus_tag="PKZ53_06630"
     CDS             complement(1751..2035)
                     /locus_tag="PKZ53_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40148.1"
                     /translation="MTDHHTDPLLDQLAREISVKVIRNLALAPSSTPIPSSSPVTVLH
                     HSELNHPSQERFLFSTDQLGEILEFLSPKACVMEPQKACDHCDICSTRGF"
     gene            complement(2078..2365)
                     /locus_tag="PKZ53_06635"
     CDS             complement(2078..2365)
                     /locus_tag="PKZ53_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNJ40149.1"
                     /translation="MLLARVIGNVVATQKNERYAGGRMLLVQPITPDGVDQGSNFLAL
                     DILDAGAGDQVIVVQEGWGASTASTGREGAAIDAAIIGIVDRIEPHPSPAT"
     gene            2548..>3697
                     /gene="coaBC"
                     /locus_tag="PKZ53_06640"
     CDS             2548..>3697
                     /gene="coaBC"
                     /locus_tag="PKZ53_06640"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNJ40150.1"
                     /translation="MSTPIRITLGVTGCIGAYKAALVLRELQKAGVEVTVVMTRHAGE
                     FVQPLTFQALSGKPVISDMWHRDQDSEIKHIALAQQTDLLLVAPLTANTLAKFAQGIA
                     DDFLSTLYLSVTAPVLLAPAMNVEMWRHPATQENLDRLRRRGVTVIEPEAGYLACGMV
                     GEGRLADPTVIAQKALELTSPRTDWAQEVVLITAGPTIEDIDPVRFLTNRSSGKMGYA
                     LAEMARRRGARVILISGPTRLSPPAGVELIPVRTTFQMYQAVLDNLPQSTVILKAAAV
                     CDFRPRAMEPNKIKKRTGLLLELEPTEDILAEIGRRRASTQVIVGFAAETDHLESYAE
                     SKLRQKNIDLIALNDVSRTDIGFDVAHNQVTLLGRDNSRRELPFLSKTE"
BASE COUNT          894 a          932 c         1031 g          840 t
ORIGIN      
        1 agactgggac atcaaggtcg ttgaaccgtg gaactgaggt tgaaaatgga tttcctgggc
       61 caggagcatg tcaggatttt catcgtcatc aagctgaaaa acggttcgat tgcgacgact
      121 ctgatattta accggatcaa tgctttggga tttaagcacg gttgccgccc gctggagaac
      181 aaagttggat tccggctggt gattggtccc aatttcaatt ggtttgccaa gcaagggttg
      241 gaacccggtg gccgatattt tgaggcagta gaggccagga ggcaggtctg ggatttgaaa
      301 ccatccgcga gcatcggtct gcgacgtggt ggtcacctga cgaaaatcct gaccggagag
      361 cacggacacc accgcaccca ccagtgggag gccgatggtg tctttcaccg taccgctcag
      421 ggcggaaagt ctttgttccg cagggatttt gggcatgtca ctcaacgcgg tcagcggtgt
      481 tccaacaaac agcgcggaaa gggcgatgag gtgaacaaat gtgctcagat tcactttcct
      541 tcgattcata ccacaacttg ctcccttgaa agtgaataaa ccgttccacc tgactgatat
      601 ctggtggaac ggaaggtaaa acccagcctg gaagatccag gtttagaagc gaatgacggt
      661 atcggggccg ccttcaatat gaacggtatc cacaaaccgg acctggtggg gttcggtttt
      721 cataatcaga gatgcggttc gttttccagt gccaaaaaag cgaacgccgc gcaggacttt
      781 tccatcggtg acaccagtca ttaaaaagag aatgtggcgg cctggcgcca gatcatcggt
      841 aaagaacacc tttgttgggt ctgataatcc catgctcttc attcgatcca gtgtttctga
      901 atcatccgtg acttttgagt gatcttcctg atggagctga tctttcaaga caaaacgggc
      961 ctggatttca ccgttgagac atttcatggc cgccgcggtc aatacacctt cgggggcacc
     1021 gccaattccc atcacagcat gaactccaga tccacccacc gccgcgctga tgccagccga
     1081 gaggtcgcca tccgagataa gctggatgcg tgctccggct tcgcggatgt cctgaatcag
     1141 tctggcatgg cgaggccggt caagcactgc gaccacatgg tcttcaatcg ttcggccaag
     1201 ccgtcgggcc aggatcttca gattttcctt aaccggagca tcaatccgca ggcaacctct
     1261 ggctgccggc ccaacgacca gtttgttcat atacatatct ggcgcattga gtaacccccc
     1321 acgttcagcc gttgccagga cggaaatggc attgggtgac ccggtggcac aaaggttggt
     1381 cccttcgagc gggtcaacag caatatcaac ggccgggtaa gggtttcctt catcgggaag
     1441 ttcggccccg atgccaacgc gttcgccaat ataaagcatt ggggctttgt cacgctcccc
     1501 ctcgccgatg acaattcgac cgttgatggg aaccgtccgt aaggcatcgc gcatggcttc
     1561 aacggccacg tggtcagaaa atttgcgttt gccctggccc atcgttcgtg acgaatcaat
     1621 tgctgcgagt tcgacaaccc gcaggaattc aagtgagagc gaagggggaa gcgactggct
     1681 tggtttcatg ctgaactcct tatgagaaaa agacgagatg gattaaaaag aaagatcgaa
     1741 gcaacactga ttaaaaacca cgggttgagc aaatatcaca gtgatcacag gctttttggg
     1801 gttccatcac acaggctttg gggcttaaaa attcaaggat ttcgccaagc tggtctgtgg
     1861 agaagagaaa tcgttcctgg cttgggtgat tgagttctga atggtggagc accgtaactg
     1921 gcgaagaaga ggggattgga gttgaggaag gagcgagcgc cagatttcgg atgaccttga
     1981 cggaaatctc gcgagccagt tgatcaagaa gtggatcggt gtggtggtcg gtcatgggat
     2041 tggaacgtcg caaaaaagga atgatgtctg ataaagttta agtcgccggc gatggatgtg
     2101 gttcaatccg atcaacaatt ccgataatgg cggcatcaat ggcggcacct tcacgtccag
     2161 ttgaagccgt cgaagccccc catccttcct gaaccacaat cacctgatct ccagctccgg
     2221 cgtcgagaat atcaagtgcc agaaaattgc ttccctggtc gaccccatcg ggtgtgatgg
     2281 gttgaaccag caacattcga ccaccagcat agcgctcgtt tttttgggtt gccaccacat
     2341 tgccgatcac gcgtgccagg agcataggag aatccggaac tgaatcaaga attgaattgt
     2401 ggaggtggat caggtaaaaa tgcaggggta tcctaccaca gggctaaagc cgaggaaaag
     2461 gatgtttcaa aaacttaagg aatgtgacac ctggccagcc caggctcgtg tgggcgcttt
     2521 ctgggtttgt ggccatcagg aggtttgatg tcgacaccca ttcgaatcac attaggggtt
     2581 accggctgca tcggagcgta caaggccgcg ctggtgttac gcgagttgca gaaagcgggc
     2641 gttgaggtga cggttgtgat gacacgtcat gctggtgaat ttgtccaacc actgacattt
     2701 caagcccttt ctggcaagcc ggtgatttcc gatatgtggc accgcgatca ggattcagag
     2761 atcaaacaca tcgcgctggc tcagcagacc gatctgctgc tggtggcgcc actgaccgcc
     2821 aataccctgg ccaaatttgc gcagggaatc gccgacgatt ttctgtcaac cctctatctg
     2881 tcggtgacgg cgccggttct cctggcaccc gccatgaatg ttgaaatgtg gcggcatccc
     2941 gcgacccagg agaatctgga tcgtctgcga cgtcgcggtg tcacggtgat tgaacctgaa
     3001 gcgggatacc tggcctgtgg aatggttggc gaagggcggc tggccgatcc gacagtgatt
     3061 gctcagaaag cactggaatt aacctccccg cggacggact gggctcagga ggtggttctg
     3121 ataacggctg gacccaccat cgaagacatt gacccggtcc gattcttgac aaaccgctca
     3181 tctggcaaaa tgggatatgc gctggctgag atggcccgcc gccgcggggc cagggtcatt
     3241 ctgatttccg gccccactcg actttcacca cctgccgggg ttgagttgat cccggtgcga
     3301 acaacattcc agatgtatca ggctgtactg gacaacctcc ctcaaagcac agttatttta
     3361 aaggctgctg ccgtctgtga ttttcgccct cgcgcaatgg agccaaataa gatcaaaaaa
     3421 cgcactggtt tactgcttga actggaacca actgaggata ttctggctga aattggtcgc
     3481 cggcgggctt cgacccaggt gattgtgggg tttgcggctg aaaccgacca cctcgaatcc
     3541 tatgccgaat caaaactcag gcaaaaaaat attgatctga ttgccctcaa tgatgtgagt
     3601 cgaaccgata tcggttttga tgtggctcac aatcaggtta cactccttgg ccgtgacaac
     3661 agtcgtcgtg aattgccatt tctttcaaaa accgaaa
//
LOCUS       DAOQDO010000330         3689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18234_length_3689_cov_3.714089, whole genome shotgun sequence.
ACCESSION   DAOQDO010000330 DAOQDO010000000
VERSION     DAOQDO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18234_length_3689_cov_3.714089"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..214
                     /locus_tag="PKZ53_06645"
                     /pseudo
     CDS             1..214
                     /locus_tag="PKZ53_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            298..1632
                     /locus_tag="PKZ53_06650"
     CDS             298..1632
                     /locus_tag="PKZ53_06650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ40151.1"
                     /translation="MHGVLAAWHEKANDEFDTRTLRLLETLATQAGMIIENERLQQEA
                     LKSEMLRQEVEIGHTIQKTLLFGQPPSYFPGLQISALTIASQLIDGDFYDFLVNSPTC
                     LDFTIGDVMGKGIPAALVGAALKNQILRSIHQLVLRDKHQAVLPGLDEILTLVHDLIV
                     PNLIELERFVTIHYGRFDLASQQFSFVDCGHTRTVHFRRETGTCHFLAGDNVPLGFLK
                     HEVYETTTVSFQPGDIFCFYSDGITETRSQEGELFGEDRLAAIIQTQADFPPKVILRL
                     VHHQLLSFAESETFGDDLTCVLIKIGEPASEGITKYQLTLDSNVTEIERVRNFTRGLC
                     AHFSQEQCPETTQTHLEIAMVEAVVNVIRHVYQEIPGHQIQISATIEPVRLTVCIEYS
                     GEWFDPDEVPEPEFDGSREGGFGLFIIRSCVDEFDLSPIPDGRVQMKLVKCF"
     gene            1721..2062
                     /locus_tag="PKZ53_06655"
     CDS             1721..2062
                     /locus_tag="PKZ53_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ40152.1"
                     /translation="MQLTVENHQGIAIVTLLGKSLDASNAKEFKRNITPVIEEYTQVI
                     FDLSKLEFVDSSGFGGILSALRQLNGKGGDLKLCSLTKTVRVLFELVRMHRLFEIFNT
                     REEALSAFTNQ"
     gene            2170..3522
                     /locus_tag="PKZ53_06660"
     CDS             2170..3522
                     /locus_tag="PKZ53_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNJ40153.1"
                     /translation="MNRHHSLHQFSRTSIWRQQWRKVRYSSGWYVATQLLSESKRVFD
                     FGLTLVLSLALVPVWVGLGVMWYAGWLEIRKTIRAGRYCEPFAAYSLHFRNPRLDRFA
                     SLIGLTELPKLLNILKGDMSLIGPRAVEPQEISPREQKARRRFDVRPGLICLWWIRQR
                     ANIDFGTEAEADSEYVETQSLKGDLGIVARSLPSLMYGAGGAPAHLDQVTMLGIPLDN
                     VTMSQAIETLMDWVTQPVQRQVTYINPHCANIACTDPHYLKSILASDFILADGIGMKI
                     AGKLLARPIRQNVNGTDLFPRLCNELQGTGHGIFLLGGKPGVADEVKQWIATQYPGVV
                     VSGTHHGYFSAAEEPQVIETIRQSGADLLLVAFGVPKQDVWIHQHLPQTGAKVGMGVG
                     GLFDFYSGRIPRAPIWMREMGLEWLYRLLQEPGRMWKRYIVGNFVFLFRVIRERLSGT
                     "
BASE COUNT          862 a          943 c          935 g          949 t
ORIGIN      
        1 gcagcttcag ccggtgtatg acaagccgat ggtgtactac cctctcacgg tgctcatcgc
       61 ctcgggcatt cgtgagttct gcctcatctc gacgccgcag gacatcgcgc gctttcagca
      121 gctcctcggc gatggaaaac gctggggtat ctccatcgag tatcgcgagc aggcaaagcc
      181 cgaaggcatc gcgcaggcct ttttgatcgc ggacagcttc aaaaagagaa agaccttgaa
      241 gcagaattca gcaaagccgc actggccgcc atcattccgg tcctggcagg tgataccatc
      301 catggagtct tggctgcctg gcatgaaaaa gcaaatgatg aatttgatac ccgcaccctg
      361 cgactgctgg aaacactggc cacccaggca ggcatgatta tcgagaatga acgactccaa
      421 caggaagcac tcaaaagtga aatgcttcgc caggaagtcg aaattggtca caccattcaa
      481 aaaaccctgc tctttggcca gccgcccagt tactttccgg gactgcagat ttcggcattg
      541 acgattgcct cacaattgat tgacggtgac ttttacgatt ttttggtgaa tagccccacg
      601 tgcctggact ttaccattgg cgatgtcatg ggcaaaggca taccagccgc tctggtcggc
      661 gccgcactca aaaaccagat tctgcgctcg attcatcaac ttgtgttgcg tgacaaacat
      721 caggcagtcc tccctggatt ggacgaaatt ctgacactgg ttcatgacct gattgttcca
      781 aacctgattg aactcgaacg ctttgtcact attcattatg ggcggtttga tttagccagc
      841 cagcagttct catttgttga ttgtgggcac acccggacag ttcatttccg ccgggaaaca
      901 ggaacctgcc actttttggc cggagacaat gtcccgcttg gttttttaaa acacgaagtc
      961 tatgagacaa ccactgtcag cttccaaccc ggcgatatct tttgttttta ttcggacggc
     1021 attaccgaaa ccagatcgca ggagggagaa ttgtttggag aagaccgcct ggccgcaata
     1081 attcaaaccc aggctgattt ccctcccaag gttatcctca ggctggttca ccatcaattg
     1141 ttatcctttg ccgaatctga aacctttggg gatgacttaa cctgtgttct gatcaaaatc
     1201 ggagaaccag ccagcgaggg cattacgaag taccagctca cccttgactc aaacgtaact
     1261 gaaattgaac gcgtccgaaa ctttaccaga ggattgtgcg cccacttttc tcaagaacag
     1321 tgcccagaaa ctacccagac gcatctggaa attgccatgg ttgaggcagt ggtcaatgtc
     1381 atcaggcatg tctaccagga aattccggga catcagattc agatttccgc tactatcgag
     1441 cccgttcggc tcaccgtttg cattgaatat agcggggaat ggtttgatcc agatgaagtg
     1501 cctgaaccgg aatttgatgg ctccagagaa ggtggcttcg gattgtttat catccgttcc
     1561 tgcgttgatg aatttgacct gagtccgatt ccagatggac gggttcaaat gaagctcgtg
     1621 aagtgttttt aacgtctggg ttccagactt atttgaaccg gttgattcca aaacaccagt
     1681 caccgccctg tttttgtctg actttgaagg agatcttgcc gtgcaactta cagttgaaaa
     1741 ccaccaggga attgcgattg ttacgctctt gggaaaatca cttgatgcct ccaatgccaa
     1801 agaatttaaa cgaaatatta cccccgtgat tgaagaatac acccaggtca tatttgacct
     1861 ctcgaaactg gagtttgttg acagttcggg atttggcgga attttgtcag ccttgcgtca
     1921 actcaatggc aaaggtggcg acttaaagct gtgttccctg accaaaactg ttcgggtgtt
     1981 gtttgagctg gtccgcatgc accggttatt tgaaattttt aatactcgtg aagaagccct
     2041 ctcggctttt accaatcagt gacaccagtc agcgtcaggg ttgaactgag cgtttgacca
     2101 ttttttggag ccgggctgtt gcgttcaacc gccttccatt ttctggactc atcattcttc
     2161 cttcacagta tgaaccgcca ccattccctg catcaatttt cgagaaccag catctggcgt
     2221 cagcaatggc gaaaagttcg ctatagctca gggtggtatg ttgcgactca gcttctgtca
     2281 gagagcaaac gtgtgtttga tttcggcctg acactggtgc tgagtctggc attggttcca
     2341 gtctgggttg gactcggagt gatgtggtat gccggctggc ttgagatccg gaaaaccatt
     2401 cgggccgggc gatattgtga accatttgcg gcctattcgc tgcacttccg aaaccctcga
     2461 cttgatcggt ttgccagcct gatcgggctg accgaactcc ccaaactgct caatattctc
     2521 aagggtgaca tgtcattgat tggcccccgt gcggttgaac ctcaggagat cagcccgcgc
     2581 gaacaaaagg cccggcggcg tttcgacgtg cgtccgggcc tgatttgcct ctggtggatt
     2641 cgccagcgcg ccaatattga ttttggcacc gaagccgaag ctgattccga atatgttgaa
     2701 acccagagtc tcaaaggtga tttaggaatt gttgcccggt cgctaccgtc cttgatgtat
     2761 ggcgcaggtg gcgcccccgc ccatcttgat caggtgacga tgctggggat cccgctcgat
     2821 aatgtcacca tgtcacaggc gattgagacg ttgatggatt gggtcaccca gccggtacag
     2881 cggcaggtta cctacattaa ccctcattgt gccaacattg cctgcactga cccgcactac
     2941 ctgaaatcaa ttctggcttc agattttatt ttagcggatg ggattggcat gaaaatcgca
     3001 ggcaagttgc ttgcccgacc aatccggcaa aatgtcaatg gcactgatct tttccccaga
     3061 ttgtgcaatg agttgcaagg caccggacac ggtatttttt tgcttggtgg aaaacccggc
     3121 gtggccgacg aagtcaaaca atggattgca actcagtatc caggcgttgt ggtgagcggc
     3181 acgcatcacg gctatttctc agccgccgaa gaaccccagg ttattgaaac gattcgacaa
     3241 tcaggcgctg acctcctgct ggtagccttt ggggttccca aacaggatgt ctggattcac
     3301 cagcatctgc cccaaactgg agccaaagtc ggaatgggcg tgggtggatt gtttgatttt
     3361 tattcgggaa gaattccccg cgcaccgatc tggatgcgtg aaatgggtct ggaatggctg
     3421 taccggttgc tccaggaacc aggccggatg tggaaacgct acattgttgg aaacttcgtg
     3481 tttttgttcc gcgtgattcg cgaacgcctg agcggcacat aaataattcc tgggagcgcc
     3541 ggcatcctgc cggcaccacg tggctcagtt tcaatcaatt gccggcagga agcccgcgct
     3601 cctaaccagg gttcttcttc tgatatgaca aagacgtctt accatttcca aaaatttatg
     3661 gtcctcgcag ggttgtgttt tttcctggg
//
LOCUS       DAOQDO010000331         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18275_length_3685_cov_3.051240, whole genome shotgun sequence.
ACCESSION   DAOQDO010000331 DAOQDO010000000
VERSION     DAOQDO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18275_length_3685_cov_3.051240"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..539
                     /locus_tag="PKZ53_06665"
     CDS             48..539
                     /locus_tag="PKZ53_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40154.1"
                     /translation="MDTTDSVLYLRGVERIGLVAYDLEHREEIVFHNLQQELLLNDAL
                     RGAVAIPGIFPPRECLAGSRKLRLIDGGVTNKLPVDKLFSPPFQPAQILAVDISNRIE
                     HRQENSLKINQLQAQFPQIPIVTVTPDTLGKATVLYRTNGLESLIESGKVVTQKLLSD
                     IAK"
     gene            complement(638..1807)
                     /locus_tag="PKZ53_06670"
     CDS             complement(638..1807)
                     /locus_tag="PKZ53_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ40155.1"
                     /translation="MRNVDYFNLDSQFTEEELMVRSMVRGFVQEQVLPVIEEAYEQGV
                     YPTQLIKAMGELGMYGANLPEEYGCAGMNSTAYGLVMQELEAGDSGVRSFASVQGSLV
                     MYPIFAFGTEEQKRKWLPEMAKGNKIGCFGLTEPDFGSNPGGMVTRAERTKYGWKLNG
                     AKMWITNGTVADVAVVWAKTGDDTRSIRGFLVEKGTAGFSAPEIKRKHSLRASITSEL
                     IFEDCEIPEENILPKVEGLRGPLSCLNQARYGIAWGAVGSAMATYNAALEYSLSRIQF
                     DRPIAGFQLTQKKLADMITEITKAQMMSLHLGRLKDQGKVTPAQISMAKRNNVAMALE
                     TARTARTILGANGISGEYPVMRHMNNLESVLTYEGTHDIHTLIIGNEVTGIQAFK"
     gene            1976..3568
                     /locus_tag="PKZ53_06675"
     CDS             1976..3568
                     /locus_tag="PKZ53_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ40156.1"
                     /translation="MLPRQVVCSGLFAIFCLNFWLASEGRGTQSRTPPLHISQTLNPE
                     PRTLNPEPCFSGYRLDLVPNDPFYFNNGNIRTDFQRWVFDGIEADRNLNAETGWGLTT
                     GNSDVVIAVIDSGVWLEHPDLKENLWRNIGEIPANQVDDDQNGFIDDVNGWDFTENTP
                     DPNPDLGNGRDDDGNGLADENTAHGTIVAGVIGARGNNRTGIAGTTWRCQLMPLKVAR
                     DDGSVSEDALAAAIRYAAKNGAQILNLSLSQATVGAAVREAIEFAQAQGALVIASAGD
                     ENTASPRFPARLPGVIAVGATDSGFRGAPRGFNGSAIAERAFFSSFGPAAVDVVAPGF
                     ALSTSVLSVFDAMSGSGNPGTPVYAFSAGTSIATGYVSGLAALIVAQAKTQNLILTAA
                     EIRYLIVTATQELPDDPDDKPDAGKTWAARGRVDFVKALNAVPTRGVLPLIRTVAFDA
                     GRRRLTVQGDNFPEADAVVTLDGAPLPRENHRYRTLSSGIARKLIVTNVDPNRLPAGR
                     SVQIAVRRVSTGQTSALFTFTR"
BASE COUNT          855 a          928 c          996 g          906 t
ORIGIN      
        1 gcaggatcac cttcgtcgac ggcaaggccg gcttctcgcc cttcatcatg gatacgacgg
       61 attcggtgct ctatctccgc ggcgttgagc ggattgggct ggtggcgtat gacctcgaac
      121 accgcgaaga gatcgtcttc cacaatctgc aacaggaact cctgctcaat gatgcactgc
      181 gtggggcggt ggcgattcct gggatttttc cacctcggga atgtctggcc ggaagccgca
      241 aactgcggct gattgatggt ggcgtgacca ataaactccc ggttgataaa ctgttttctc
      301 caccgtttca gccagcccaa atactggcag ttgatatttc aaaccgaatt gagcaccgtc
      361 aggaaaacag tctcaaaatc aatcagttac aggcacagtt tccccagatt ccaattgtga
      421 cagtcacgcc ggacacactt gggaaagcga ctgttttgta tcgaaccaac ggcctggaat
      481 cattgattga atctggcaaa gttgtgactc agaagctttt gagcgatatc gcgaaatagt
      541 cagtaccacc cgcgttagca ggtggtactg acttttagac aaaaaaaggg cactcccgca
      601 cggggagtgc cctttcaagt gttggggaag ggaactttta cttaaatgcc tgaatgccag
      661 tgacttcgtt gccaatgatg agggtgtgga tgtcgtgtgt tccctcatag gtgaggactg
      721 attcaaggtt gttcatatgg cgcatgaccg gatattcgcc gctgatgcca ttggcaccaa
      781 ggatggtgcg ggcggtgcgg gcggtttcga gcgccatggc gacgttgttg cgcttcgcca
      841 ttgaaatctg ggccggagtc actttgccct gatctttgag ccggccaagg tgcagcgaca
      901 tcatctgagc tttggtgatt tcagtgatca tgtcggccag tttcttttgg gtaagctgga
      961 atccggcaat cgggcgatca aactggatgc gtgacaggga atattccagc gcggcattgt
     1021 aggttgccat cgctgaacca actgctcccc acgcgatgcc gtatcgagcc tgattcaaac
     1081 aggaaagtgg tccgcgaagt ccttcgactt taggcagaat attttcttct ggaatttcgc
     1141 aatcttcaaa aatcagttcc gaggtaatcg aagcgcgcag tgaatgcttg cgcttgattt
     1201 ctggagctga aaacccagcc gtgccttttt cgaccaggaa gccacgaatg ctgcgggtgt
     1261 catctccggt tttggcccag acgaccgcga catccgcgac cgtgccgttg gtgatccaca
     1321 ttttggcgcc gttgagtttc cagccgtatt tggtccgttc ggcacgggtc accattccgc
     1381 ctggattgga tccaaaatcc ggttcggtca gaccaaagca gccgattttg ttgcctttag
     1441 ccatttcggg cagccatttg cgtttttgtt cttcagttcc aaacgcaaaa atcggataca
     1501 tcaccagcga gccctgcact gaagcaaagc tgcgcactcc ggaatcaccg gcttcgagtt
     1561 cctgcatcac cagcccatag gcggtgctgt tcattccagc gcacccgtat tcttcgggaa
     1621 ggttggcgcc atacatgccc agttcgccca tggctttgat caattgggtt ggatacacac
     1681 cctgttcata ggcttcctcg atgactggca gcacctgttc ttgaacaaag ccgcgaacca
     1741 tcgagcgaac catcagttct tcttcggtga actgcgaatc caggttaaag taatctacat
     1801 ttcgcatgag ataaaactcc tcaaaatttc ggaccagcaa ggtcaaaggt tgatcactcc
     1861 caacggcgat gggggaagaa atgaatgcgt tgcgataaag tgaaacgata tgatgagaac
     1921 gccgtcagaa tgaaggttga atgcaatgcg agaggatacc attggaattt tcattatgct
     1981 acctcgacaa gtagtctgta gtggcttgtt tgctatcttt tgcctcaatt tttggcttgc
     2041 cagtgaaggg cgcggtacac aaagccgtac accgcccctc cacatttccc aaaccctgaa
     2101 ccctgaaccc cgaaccctga accctgaacc ctgcttcagc ggttaccgcc tcgatctggt
     2161 tccaaacgat ccgttttatt tcaacaacgg caatattcga actgactttc aacgctgggt
     2221 ttttgatgga attgaggctg accggaatct gaatgctgaa actggctggg gattgaccac
     2281 cggcaactca gatgtggtga ttgcggtgat tgattcgggt gtctggctgg agcatccaga
     2341 cctgaaagaa aacctgtggc gcaacatcgg cgaaattcca gccaatcagg tggacgatga
     2401 ccagaatggc ttcattgatg acgtcaatgg ttgggacttt accgagaaca caccagaccc
     2461 aaatccagat ttgggaaatg gccgcgatga cgacggaaat ggcctggccg atgaaaacac
     2521 cgcacacgga acaattgtgg cgggtgtgat tggggctcgc ggaaataatc gcaccggcat
     2581 tgctggtacc acgtggcggt gtcagttgat gccactcaag gttgctcgcg atgatggatc
     2641 ggtgagtgaa gatgccctgg ccgctgccat tcgctatgcc gcgaaaaatg gcgcgcagat
     2701 tctgaacctg agtttaagtc aggcgacggt gggtgccgct gttcgtgaag ccattgaatt
     2761 tgcccaggct caaggtgcgt tagtgattgc ttccgccggg gatgaaaaca cagcttcacc
     2821 acgatttccg gcccggttac ccggtgtgat tgccgtcggc gccactgaca gcggctttcg
     2881 cggagcgccg cgtggtttta atggatcagc cattgccgag cgggcttttt tcagttcgtt
     2941 tggtccggca gcggttgatg ttgtggcgcc agggtttgcc ctctcgacat cagttctcag
     3001 tgtgtttgat gcgatgtctg gcagtggaaa tcccggaacg ccggtgtatg cgtttagtgc
     3061 tggcacatca attgccaccg gatatgtctc ggggttggcc gcattgatcg tggcgcaggc
     3121 caaaacacaa aacctgatac tgacagcagc agaaattcgc tatctcattg tgacggcaac
     3181 tcaggaactt cctgacgacc cagacgataa accagatgct ggaaagacct gggcggcacg
     3241 cggtcgggta gatttcgtga aagcgctcaa tgctgtacca acgcgaggtg tcttgccact
     3301 catccgaacc gtggctttcg atgccgggag gcgtcggttg acagtgcagg gtgacaattt
     3361 tccggaagct gacgccgttg tgacactcga tggcgcacca cttccgcgag aaaaccaccg
     3421 ctatcgaaca ctgagttcgg gaattgcccg gaaattgatc gttaccaatg ttgatcccaa
     3481 tcggcttccg gctggaagat cggttcaaat tgccgttcga cgcgtttcaa ctggccaaac
     3541 cagtgcgttg tttactttta ctcgttgata agtcagtagt cagtagataa aacccagatc
     3601 gttgaatccg tggaattctt gaaaagaatt gtttttaaat gattcaaata gagccaaatc
     3661 tatttgatag catttcgaag tccca
//
LOCUS       DAOQDO010000332         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18345_length_3677_cov_2.756764, whole genome shotgun sequence.
ACCESSION   DAOQDO010000332 DAOQDO010000000
VERSION     DAOQDO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18345_length_3677_cov_2.756764"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKZ53_06680"
     CDS             complement(<1..220)
                     /locus_tag="PKZ53_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40157.1"
                     /translation="MARPTKFTPDRLEKLASALEAGKSLRQAMKVIGIPRSCFYATLF
                     QKKYRDIVWKLKKCRVIGDRKKLVCSAVK"
     gene            complement(501..920)
                     /locus_tag="PKZ53_06685"
     CDS             complement(501..920)
                     /locus_tag="PKZ53_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40158.1"
                     /translation="MNYNEAMAELDVIHLQMSMIWAQLGGDPWQSDEFRELQSRSSVL
                     SRQLPKLRAADMLEKIGTIEQDLAVNPSAYSELILMKLRLDYQLLCRNIEIQERLEDL
                     EKQQNQELKQLPPPPPSASSLARRKPRAHRSIWKGKQ"
     gene            976..1108
                     /locus_tag="PKZ53_06690"
     tRNA            976..1108
                     /locus_tag="PKZ53_06690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1066..1068,aa:Val,seq:cac)
     gene            1392..2024
                     /gene="ribA"
                     /locus_tag="PKZ53_06695"
     CDS             1392..2024
                     /gene="ribA"
                     /locus_tag="PKZ53_06695"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNJ40159.1"
                     /translation="MSAKIDSPQSYSTVEFVAEANLPTEMGTFRIAGFRSLTSDEEFV
                     VLLKGNPTPHQASLVRIHSQCLTGDVFHSIKCDCGRQLKRAMEMIAEAGHGAIVYQHQ
                     EGRGIGILNKIRAYALQDQGADTIEANVQLGLEVDSREYSQCAEILKQLGFHQIRLMS
                     NNPQKISALRRFGLQVVERVPIEVASEPDAPAVNYLKTKKEKMGHLLNLV"
     gene            2045..3088
                     /gene="moaA"
                     /locus_tag="PKZ53_06700"
     CDS             2045..3088
                     /gene="moaA"
                     /locus_tag="PKZ53_06700"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNJ40160.1"
                     /translation="MLLPLPVLNFPSQTNPQSGVLRDSYGRVIRDVRISVTDRCNFRC
                     TYCMPSEGLTWKPKSEILSFEEILELASILVELGITKIRVTGGEALLRRGVVNLIQDL
                     ARLKPVEDLALTTNGFFLKEFTPDLVRAGLKRVTISLDSLNPQGFQSLTKVNALPRVL
                     EAIDVAQRAGLSPVKINAVLIRGFNDGEIEDFAAFAREWGVSVRFIEYMPLDGPGEWS
                     RAQVVPGREVYQRIQAQFPLVPLSAQDASETARRFRFADGTPGEIGIIAPVTEPFCGA
                     CSRIRLTADGKLRTCLFSLTEYDLRAPLRRGATRQELIELIREAVRLKEAGHRINQSD
                     FVPPERTMSCIGG"
     gene            3115..3426
                     /locus_tag="PKZ53_06705"
     CDS             3115..3426
                     /locus_tag="PKZ53_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNJ40161.1"
                     /translation="MAQTVGEIFKGMKSAYKKGAFKEEKSFYFSLGDDKWTVIVGPKK
                     CVVEAGKTIEQADCVLKTSPELFVKMWNREHTPGMGDFMSGRVKSNDPSALKTFLDAF
                     D"
BASE COUNT          898 a          846 c          912 g         1021 t
ORIGIN      
        1 ctttgactgc tgaacaaacc aattttttgc ggtcgccgat gacccgacat tttttgagtt
       61 tccagacaat gtctcgatat tttttttgaa ataaggttgc atagaagcac gaacggggaa
      121 ttccgatcac tttcattgcc tgacgcaatg actttccggc ttctaacgct gatgcaagtt
      181 tttctaatcg gtcaggggta aatttggtag gccgggccat gagtaggact tcctccacaa
      241 aaaggatgga atggatgtga ccggaaaatc ctactgagga aaatttaatc gttagtgagt
      301 tgttgggcaa ggccgaacag caggttgtga aatttcaaaa aactctcggc catcccaggc
      361 attatgagtg actatccatt ccccatttgg caattgctca actgaaggct gacatttaca
      421 gattggatga cacatgcgtc ttttaatgtc attgcggggg atgagatgga taaagttcac
      481 agggcctggg ttcgtaaatg tcattgtttt cccttccaaa tgcttctgtg agcgcgaggc
      541 ttacggcggg ctaaactaga agctgaggga ggtggcgggg ggagttgttt tagttcttgg
      601 ttttgttgtt tttctaaatc ctctaagcgt tcttgaatct caatgtttcg acataacagt
      661 tggtaatcaa gccgcagttt catcaagatt aattctgaat aggccgaagg atttacagcc
      721 aagtcctgct cgattgtgcc aattttttcc agcatgtcag cggctcttag ttttggtaac
      781 tgtcggctaa gaacactgga tcgggattgc agttctcgaa actcatcgga ctgccaggga
      841 tctcctccca attgtgccca gatcatggac atctgtaaat gaattacatc gagttctgcc
      901 atggcttcat tgtagttcat aaatactgtg acaaatttgt gacaaaaacc tggattagct
      961 agtggtaacc tatgggggtt ggtttggtct tctggctgtt tactggctaa ctggtttggg
     1021 cttaaatacc ctgaaaatga ctggttaaaa attaaccagt cgcttcacac gcgagaggtc
     1081 acaggttcga gtcctgtacc gcccaccact ttcattttac ttccagccat tcattctctt
     1141 ctaaccactt ctgagatgct gattatttcc tgataccagg ttcctggtcg tgtgcttttt
     1201 cctctaaaag cttctttatt ctctcccaaa aatgaaccca aaattcttaa ccttctggct
     1261 atcatagggt tggaatactt tgcttgcgct cctccagtct gaaccttact atccagagtt
     1321 gaaacccctt tacacttttg gctcgctcaa acgtccaaaa gtttcttgaa tagtagttct
     1381 ggagagaaag catgagtgca aaaatagaca gtccgcaaag ttactcaacc gttgaatttg
     1441 ttgccgaagc caacctgccg acggaaatgg gtacgtttcg gatcgcaggc ttccgaagcc
     1501 tgaccagtga tgaagaattt gttgttttgt tgaaaggaaa cccaacaccc catcaagcca
     1561 gtctggtgcg catccattcc caatgcctga cgggcgatgt atttcattca atcaaatgtg
     1621 attgtggccg gcaattaaaa cgggccatgg aaatgatcgc cgaagctggc cacggcgcga
     1681 ttgtgtacca gcaccaggaa ggtcgcggca tcggaatttt gaacaagatt cgcgcctatg
     1741 ccctccagga tcagggggcc gacaccatcg aagccaatgt ccagttaggt cttgaagttg
     1801 attcccgcga atattcccag tgtgctgaaa ttctaaaaca gcttggattc catcagattc
     1861 gcctgatgtc aaataaccca cagaaaatca gtgccttgcg gagatttggc ctgcaagtcg
     1921 ttgagcgtgt cccgattgaa gttgcctctg agccagatgc cccggcggtc aattatctca
     1981 aaaccaaaaa agaaaaaatg ggccatttgt tgaatctggt gtaacccaca ctgtgtgatt
     2041 gcctatgttg cttccattac cagtgctcaa tttcccgtct caaaccaacc cccaatctgg
     2101 tgtgctccgg gatagttatg ggcgggttat tcgggatgtg cgcatctcgg tgaccgaccg
     2161 atgtaatttt cgatgcacct actgtatgcc gtcagaaggg ctgacctgga aacccaagtc
     2221 agaaatcctg tcatttgaag aaattctaga gctggcctca attctggtcg aactggggat
     2281 taccaaaatt cgggtgaccg gtggggaagc attacttcgc cgtggggtgg tcaatctcat
     2341 tcaggatctg gccagactca aaccggttga agacctggcc ttaaccacca acgggttctt
     2401 tttaaaggaa tttaccccag acctggtccg ggctggtctc aaacgggtca ccatcagcct
     2461 tgattccctc aatcctcagg ggtttcaatc gctcaccaaa gtaaatgcgc tgccgcgagt
     2521 cctggaagcc attgatgttg ctcaacgggc tggactctca cccgtgaaaa tcaatgctgt
     2581 gctcattcga ggctttaatg acggggaaat cgaagatttt gcggcctttg ctcgtgagtg
     2641 gggtgtttca gtgcgcttca ttgaatacat gccgctggat gggccaggtg aatggagtcg
     2701 ggcgcaggtg gttccgggac gggaagtcta tcagcggatc caggctcaat ttccgctggt
     2761 gccgctatct gcacaggatg ccagtgaaac tgcgcgtcgg tttcgatttg cagacgggac
     2821 accgggtgaa atcggaatca ttgccccagt gaccgaaccc ttctgtggtg cctgtagtcg
     2881 gattcgattg acggctgatg gaaaacttcg cacttgcctt ttttcgctga ctgagtatga
     2941 cttacgggca ccgctccgtc gcggcgcaac tcgtcaggaa ttgattgaac tgattcgcga
     3001 agctgttcgg ctcaaagaag ccggccatcg gattaaccag tcggattttg tcccgccaga
     3061 gcggacgatg tcgtgcattg gtgggtagcc tgggcaattt tcttggagga atttatggct
     3121 caaacggtgg gtgagatttt taagggaatg aaaagtgcct ataaaaaagg tgcgtttaaa
     3181 gaagaaaagt cattttattt ttcacttggc gacgataaat ggacggtcat tgtcggtccc
     3241 aaaaaatgtg ttgtggaagc cgggaaaacg attgagcagg ctgactgtgt gctcaaaaca
     3301 tcaccggaac tctttgtcaa aatgtggaat cgtgaacaca ccccaggaat gggcgatttt
     3361 atgtcggggc gggttaaatc aaatgatcca agtgccttaa agacatttct ggacgccttt
     3421 gactaaagca acctcctgca agtccaaaac ctctcttaac ttctgctgaa agtattccaa
     3481 cgtttattgt gaattccgag ccaaagagcg ggtcaccagg ttgaacctac gcagcctgga
     3541 aggtgtggct tgctccctcc tcgctctttg ctctggcttg atggcgttca attcatatgt
     3601 ctggggactt tatgtatcaa acacgctctc tttctttctt cctctgtttg ggcttatgcg
     3661 ctgtttctgt gggcata
//
LOCUS       DAOQDO010000333         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18363_length_3675_cov_2.713260, whole genome shotgun sequence.
ACCESSION   DAOQDO010000333 DAOQDO010000000
VERSION     DAOQDO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18363_length_3675_cov_2.713260"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKZ53_06710"
     CDS             complement(<1..1441)
                     /locus_tag="PKZ53_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ40162.1"
                     /translation="MSSPQSQSRPASRRLFQPAIGFVLSLSCCLLLGWHGFPVNKPVA
                     AVSQPQKQSETSSISLFAAGRGNPYVNVTDGRPLSTLRALTPRAETTSSTAQLTPLAL
                     TSGDFTGDGLPDLVSTYTDGFQGIVRLHPGNMDAHDPNSPVALAHKQAGMFSDESFLP
                     PLAETSIPQPAHFVETGDFNADGRLDVVTAAQGGTALFWLAGNGQGGFEAPVMIEVDG
                     LITAFAGGEINQPDGARDLVVAITRGDTALALVFESITGGLFGKPEPIRLPFPAPSLA
                     LGRVNDDSWSDLVVAAGTELLVIQGRDRLSVLNEPSFNAALKTRRIFSEPVVSVVTGR
                     FTSTTHDDIALSTQSGEVLVLDDVDRRRHELPESENEASTETRRPVVEQTRIPLEQWQ
                     RHSLGNIIPQARLVATKTSSLPTDDLMVVGAGSQLRLLTAVGKVDSAYSGLDSELVEL
                     SAFTPPVAAIPMRLNGDGLDDLVILSDTEQ"
     gene            1850..2914
                     /locus_tag="PKZ53_06715"
     CDS             1850..2914
                     /locus_tag="PKZ53_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40163.1"
                     /translation="MRQVQQDTYFVPVIQNTPPLPSIDWSLIGQQAPVLLDTPAGVLP
                     QADVILITWSPTEWAALEQVFCNSSGTMPYSEQNTAYWSGWQIYNWGMPPYSGSDPWT
                     YWGYYRLVQIQGKNVLLFKSNTHYPWPGPTYLEEMVANLIAAVQPSLVMSTGTAGGAQ
                     VYDHTGTVRVIRAATLYLPYVPQSDWITYQDSWLANWSVAGNTNFDKLLFPVPTTESD
                     LESIAEQFNQYYGTDYTLSQFDPNNLDMGDPIPLIYNMTPSGTSVLTTATFIVGNTDG
                     NYANFACIEMDDAIVARACFKHTTLFGFVRNLSDPVSNAALPVDVQSNWGSALYNAYG
                     FYTSFNSALTAWAIISGQLG"
     gene            3388..>3675
                     /locus_tag="PKZ53_06720"
     CDS             3388..>3675
                     /locus_tag="PKZ53_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40164.1"
                     /translation="MLQADKIGEYYLSYFTSRFLRSNLLSLGINPETDPKELEKLLTA
                     KLGKINQRKETALQHMKDLANGTFWTTPDRTEVLCLTEQGGRDWFRRDGGNW"
BASE COUNT          910 a          896 c          965 g          904 t
ORIGIN      
        1 gctgttcggt gtcgctcaaa atcaccagat cgtccaggcc atccccattc aatcgcattg
       61 gaatcgcggc aactggtggc gtaaaagctg aaagttcaac cagttccgag tccaatccag
      121 aatatgcaga atccactttt ccaacggctg tcagcaatcg aagctggctt ccggcaccga
      181 ccaccatcag gtcatccgtc ggaaggcttg aggtttttgt tgctacaagt ctggcctgag
      241 gaatgatgtt cccaagagag tgccgttgcc attgttcgag tggaattcgg gtttgctcaa
      301 ccaccgggcg acgtgtttct gtggacgctt cgttttcaga ttccggaagt tcgtggcggc
      361 ggcggtcaac atcatcgagt accagaactt ctccactttg agttgagagg gcaatgtcat
      421 cgtgggtggt gctggtaaag cgaccagtca ccacactgac aaccggttct gaaaaaatgc
      481 gtcgagtctt gagtgcggcg ttaaacgacg gttcatttaa aactgaaaga cggtcacggc
      541 cttgaatcac cagcaactcg gttccagccg ccacgaccag atctgaccag gaatcatcat
      601 tgacgcgccc cagcgccagg ctgggagccg ggaagggaag ccggatcggt tcaggttttc
      661 caaacaaacc tccagtaatt gattcaaaca ccagtgccag cgcggtgtct ccgcgagtga
      721 tcgcaaccac gaggtccctg gcgccatcag gctggttgat ttcgccgccc gcaaaagccg
      781 taatcagtcc atccacctca atcatcaccg gcgcttcaaa tccgccctgg ccatttccgg
      841 caagccagaa cagcgcggtt ccaccctggg ctgctgttac cacatcgagc cggccatcgg
      901 cgttaaagtc tccagtttca acaaagtgtg ccggttgtgg aatcgaagtt tcagcaagtg
      961 gcggcaggaa tgattcatct gaaaacatcc cggcttgctt gtgagccaga gcaacaggtg
     1021 agttgggatc gtgtgcgtcc atatttccag gatgcagccg cactatgccc tgaaagccgt
     1081 ccgtgtacgt gctcaccaga tcgggcaggc cgtctccggt aaaatcgccc gaagtcagtg
     1141 cgagtggcgt gagttgggcg gtggaactgg tggtttcagc cctgggtgtc aaggcacgca
     1201 aggtactcaa cgggcggcca tctgtcacgt tgacataggg attcccacgc ccagccgcaa
     1261 agagcgaaat ggatgaggtt tcagactgtt tttgaggttg agacactgcg gcgacgggtt
     1321 tattgaccgg gaacccgtgc catccgagca gcaaacagca actcagactg agaacaaatc
     1381 caatggctgg ttgaaacaaa cgtcgggagg caggacggct ctggctttga ggggaggaca
     1441 tacgaattgg ctccagggca aaccttgtca gtcttgtggg gcagaagaaa ctgaccagtg
     1501 tggtggttga gaggaagacg taaagcaacc aggggcggcg cggcaaacca taccctgatt
     1561 gagttgagcg tctcgtaact gcgattttat ggaacctgga tcaagtaaaa agagtgctga
     1621 gttttttgag tggagcggcg tcatcatatg ccgattaccc gcaaatttga agacaataaa
     1681 tggctttgga gcgcgccgac ttgtcggcgc tttcaccttt ggcgacttgt cgccagttca
     1741 acaaggtttg tccttgattt tgacttgatc aacagtaatt gttcgaacga tttgtttgag
     1801 aaaccatcgc gattgctaaa aacaggactt cactacagga gttgatgcca tgcgacaagt
     1861 tcaacaggac acctattttg tgcccgtgat tcaaaacaca ccgccgttgc cttcgattga
     1921 ctggagtttg attggccagc aagcaccggt tttactcgac acacccgctg gagtgctccc
     1981 ccaggcggat gtgattctca ttacctggtc gccgactgaa tgggcggccc tggaacaggt
     2041 tttttgtaac agcagcggta cgatgcctta ttcagaacaa aacactgcct actggagcgg
     2101 ctggcagata tacaactggg gaatgccgcc gtattcaggt tctgatccct ggacgtattg
     2161 ggggtattac cggctggttc aaatccaggg aaagaacgtt ttattgttca aatcaaacac
     2221 gcattatccg tggccggggc caacctacct ggaggaaatg gtggcgaatt tgattgccgc
     2281 tgtgcagcca tctctggtta tgtcaaccgg cacggctggt ggagcgcagg tctatgacca
     2341 cactggaacg gtgcgggtaa ttcgtgccgc aacgctgtat cttccgtatg ttccgcaatc
     2401 tgactggatt acctatcagg attcgtggct ggcgaactgg tcagtggctg gaaacacaaa
     2461 ttttgacaag ctgttgtttc cggtgccgac gacggaatcc gacctggaat ccattgccga
     2521 gcaattcaac cagtactatg ggactgacta cacgttgagc cagtttgacc caaacaatct
     2581 ggatatggga gaccccattc cgttgattta taacatgaca ccgtcgggga cttcggtgtt
     2641 gacgactgcg acgtttattg ttggaaatac tgatggcaat tatgccaatt ttgcctgtat
     2701 tgagatggat gacgccatcg tcgcacgagc ctgtttcaaa cataccacgt tgtttgggtt
     2761 tgtacgaaac ctttcagacc cggtgtcaaa tgctgccctc ccggttgacg tccaaagcaa
     2821 ctggggaagc gctttgtaca atgcctatgg gttttatacc agtttcaaca gtgcgttgac
     2881 tgcgtgggca attattagtg ggcagttagg gtgaggcggg attgggttcc ggggtttcga
     2941 gtttttgtcc tttttgtctt ttcgaaagtc cggaccccgg acccctaatt ctcaaaatcc
     3001 atcatgctgg attcggcgtg gcagggcctt cctgtttaca aaaagtgttt gtttaggatc
     3061 aaagggatcg aaatagtttg attcattcaa ggaaaagatg ttgtaattga ctctcactga
     3121 tgcacaaatg tgttttaacc tccggaagtt gagtcttgtt acaatgagga tatttatgaa
     3181 aagacaaaca atgctcagac aagttacgtt gataattcta ctacttatcg gattttatac
     3241 ctggaacggc tccataagta agaccattca tgccagcagt tcaatcaacc ttctgtcacc
     3301 tcccaacaac ctccaattcc agggctgctc cctgcctcag atcaacaaat cacggaaatc
     3361 aaagcctttt tagcgcgcca atcctcattg ttacaggctg ataaaatagg cgagtactat
     3421 ttgtcatatt ttaccagccg atttttgcgg tcgaatttat tgtccttagg aattaacccg
     3481 gaaacagatc caaaagagct tgaaaaactg ctcactgcaa aattgggcaa gattaatcaa
     3541 agaaaagaaa ctgctcttca acatatgaaa gacttggcca atggtacttt ttggacaact
     3601 ccagatcgca ccgaagtcct ttgcctcact gagcagggag ggcgagactg gttcagacgt
     3661 gatggaggca attgg
//
LOCUS       DAOQDO010000334         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18378_length_3673_cov_2.913212, whole genome shotgun sequence.
ACCESSION   DAOQDO010000334 DAOQDO010000000
VERSION     DAOQDO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18378_length_3673_cov_2.913212"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1474
                     /locus_tag="PKZ53_06725"
     CDS             407..1474
                     /locus_tag="PKZ53_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNJ40165.1"
                     /translation="MIEEIRLVQASGNVTVFPANGGSVVPGTSGMSLYEGDRIQTGSG
                     STASLQFVDGSTLRIKENSTVIVKRNSKELKSDKTQVENNVAFGTVNMSNPVSTPAAG
                     SVNNVTAPGVSVSFQSGTQGTINYDGQTTKVVSEKGSLNVVTDKGNAVVVSENRGSEF
                     QEGAKVNDIAYLPSPKLRSPENSELRSFNQGTSPEIVFTWSPVPGAVSYNFQLATSNS
                     FVKSTIIAERNQRGTSTSNVFRPYDGNYYWRVQGVEGNGKEGIWSNEQRLQIVIRASS
                     IIDTPIYLPRPTYREVGRGIYEVNGETEPGVQLRINGKNVLVGPDGKYMTNVDLNGSQ
                     SVIFFAQDSKGRKRTENYPPR"
     gene            1869..3302
                     /locus_tag="PKZ53_06730"
     CDS             1869..3302
                     /locus_tag="PKZ53_06730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNJ40166.1"
                     /translation="MRVAVVYLEKIPEVLLRVLGHTASIETALFTGKRNDEDFLSLWH
                     PEVILYELEDEASEQLSRRMLAHARHAVAGIPAFSFSSTAGADMRSLSMEIGFINHLE
                     ALRLTPRELELHLQYGLRLARIPQPAVPLVTGRGSFDKAFEAVTSFHSSLDQAKVVES
                     AMLHLPRLVPADTWALYLSTESGDHLNLVAAVNASGRAIVRSVSMNDEENPLVQTVRE
                     EKSMFVIFPAAGATPPDPVITFSLSALSDKYETQLLLPLLTNGNLCGVMMASLEAGTR
                     TFTAENLDIAERVSASLAVALGNAISFTKAESMCQLDDLTSLYNARFFYQALDTELKR
                     SRRYGLHLAVIFLDLDGFKGINDQFGHLVGSNTLVEVGQLIYRAVRETDVVARYGGDE
                     FVIVLPETNAAQAFIIAERIRARIEATTFQKGRGLTVHLTCSLGISAFPEHGTVAEDL
                     VKWADQAMYNAKQGTKNQVVVVNLQGA"
     gene            3525..>3673
                     /locus_tag="PKZ53_06735"
     CDS             3525..>3673
                     /locus_tag="PKZ53_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNJ40167.1"
                     /translation="MKLRSLFNFFSNDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     ITNKV"
BASE COUNT          924 a          860 c          945 g          944 t
ORIGIN      
        1 tttttcagca ttcttcgcac ggcaaacgac aacttcaact ttcataagat ttggcggcga
       61 cgatctggat cagaacgcag ggatcattcg aatgtgaaac tgcgcgaccc caggttagac
      121 ggcgatggtc tggcacaagc cctatcgtca gcatctttcg cggtgcctca gtttgacgac
      181 ccgcactcat tttggaagga tgatctggat tttctgatcc aatatcggag gagacgtgaa
      241 cagatcaggt caggattccc gcatacggcg ggaatatttt gattggtatg tcatttcaaa
      301 gaaaactctg tacacaattg gggggattct tgtcctcatc atagggttaa ttcttttttg
      361 gatttggtgg aaatatttaa agcctgttga tcctcctcct gagattgtga ttgaagaaat
      421 ccgtctggtt caagccagtg ggaatgtgac tgtttttccg gccaatggtg gcagtgttgt
      481 ccctggtact tctggaatgt cattatatga gggagatcgg atccaaaccg gatcggggtc
      541 aacagcttcg ctccagtttg ttgatggtag taccctccgg attaaagaaa attcaaccgt
      601 catcgtaaaa cgaaacagca aagagttaaa gtctgataaa acccaggtgg aaaacaacgt
      661 ggcgtttggc acggtcaata tgtcaaaccc ggtgtcaacc ccggcggctg gaagtgtgaa
      721 taacgtgacc gcgcctgggg tatcggtttc gttccagagt ggaacacagg gcaccatcaa
      781 ctatgatggt caaaccacca aagttgtatc cgaaaaaggc agcctgaacg tggtgaccga
      841 taaaggaaat gcggtggttg tctccgaaaa ccgagggagt gagtttcaag aaggcgccaa
      901 agtcaatgat attgcctatt tacccagtcc aaagctgaga tcacctgaaa attctgaact
      961 tcggagtttt aaccagggca catcgcctga aattgtgttt acctggagcc cggtgccggg
     1021 ggctgttagt tataacttcc agcttgctac ctcaaactcc tttgtcaaga gcacaattat
     1081 tgctgaacgc aatcagcgtg gtacctcaac cagcaatgtt tttcggccat atgatggcaa
     1141 ctattactgg cgggttcagg gggttgaagg caatgggaaa gagggaatct ggtccaatga
     1201 gcagcgactg cagattgtga ttcgggcaag cagtatcatt gataccccaa tatacttacc
     1261 ccggccaacc tatcgtgaag tgggtcgtgg gatttatgaa gtcaatggcg agacggaacc
     1321 gggtgttcag ttgcgaatca atgggaagaa tgtcctcgtc ggcccggatg gaaaatacat
     1381 gaccaatgtt gatttgaatg gaagccaatc cgtcatcttc tttgcccagg attcaaaagg
     1441 acgaaaacga accgaaaatt atccacctcg ataacgggtc aatcagtccc agggcaagga
     1501 cccgggtggg cttttgctcc aggcactcaa tcccaaaaaa atcttccagg cagggagccc
     1561 tccttgcctg gctccttgaa aaacacaacc gctggttcca tagtgttttc aacgttttgt
     1621 caaaaactca gtagacacgc gcgtggaaac tgagtactgt cacatgtttc gcggaacatg
     1681 gtggattggt gctttggaca acctgaggtc agcggtttgc ggttggaaga tttccttctt
     1741 ttcccattta actgatgcac tccaaacagt tgtttcagtt caaggaacct cttgatcgca
     1801 ggccctgata ccaaatcatc atccacattt ttctccccct cacttcattt tggttgatag
     1861 agactgcaat gcgcgtggcc gtggtgtacc tggaaaaaat ccctgaagtt ctcttgcggg
     1921 tccttggcca taccgcgtct attgaaacgg cattgtttac cggcaagcga aacgatgaag
     1981 atttcctgag cctgtggcat cccgaagtga ttttgtatga actggaagat gaagccagtg
     2041 agcaattatc acgtcgaatg ctggcccatg cccggcatgc ggtggctgga attcccgctt
     2101 tttccttttc atcaactgct ggtgccgata tgcgaagtct ctcgatggag attgggttta
     2161 tcaatcatct tgaagccctc cgattgacac cacgcgaact tgaattgcat ctccagtatg
     2221 gcttgcgcct ggcacgaatc ccacaaccgg ctgtgccatt ggtaaccgga aggggcagct
     2281 ttgacaaagc ctttgaagcc gtgacgtctt ttcactcgtc gcttgatcag gcgaaagtgg
     2341 tcgaatcagc catgcttcat ttgccgagac tggtgcctgc cgatacctgg gcactctatc
     2401 tcagtactga atcaggtgat catttaaatc tggtggccgc cgtcaatgct tcggggcggg
     2461 cgattgtcag aagtgtttcg atgaatgatg aagaaaaccc gctggtccag actgtgcgcg
     2521 aagagaagtc aatgtttgtc atctttccgg cagccggggc aaccccccca gacccggtga
     2581 ttaccttttc cctgagtgcg ctcagcgata aatacgagac tcagttgctg ttgcccttac
     2641 tgaccaatgg caacctctgt ggagtgatga tggcgtcgct ggaagccggt actcgcacct
     2701 ttaccgctga aaacctggat attgctgaac gggtcagtgc ctcactggcg gtcgccctcg
     2761 gaaacgccat cagttttacc aaagcggaaa gcatgtgcca gttggatgac ttgacaagcc
     2821 tgtataatgc caggtttttc tatcaggcac ttgataccga actcaaacgg tcacgtcgct
     2881 atggccttca cctggcggtg atttttttag atttagatgg attcaaggga atcaacgatc
     2941 aatttggcca cctggtcgga agcaatacac tggttgaagt cggtcagttg atctaccggg
     3001 ccgtacgcga aaccgacgtg gttgcccgct atggtgggga tgaatttgtg atcgtattgc
     3061 cggaaaccaa tgctgctcag gcatttatta ttgccgaacg gatccgggcc cgcatcgagg
     3121 ccaccacctt ccaaaaagga cgtgggctca cggttcatct gacctgttcg cttggaattt
     3181 cggcctttcc cgagcatggc actgtcgctg aagatctggt caaatgggca gatcaggcga
     3241 tgtataatgc caagcaaggg accaaaaatc aggtcgtggt ggtcaatttg caaggggctt
     3301 agtggttggg gcttggggct tggggcttag ggcttgggtt tcggaacaga tactttccaa
     3361 aaatcaggaa cgaagaacca gaaaccagga acgaagagaa ccaaaaatcc tttgatattc
     3421 aactacttaa ctggagaatc tccaaattgg ggtttcggat tgataaattt ttgatagatt
     3481 ggtgagttaa catttcctgc ttgaaaggtt gctgtacttc gtccatgaaa cttcgctctc
     3541 tgtttaactt cttctcaaat gacctcgcga ttgatctggg aaccgcaaat accctggtat
     3601 atgccaaggg tcgcgggatt gtggtcagtg aaccatccat cgtcgccatc aacaaaatca
     3661 ccaacaaagt tga
//
LOCUS       DAOQDO010000335         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18382_length_3673_cov_2.501658, whole genome shotgun sequence.
ACCESSION   DAOQDO010000335 DAOQDO010000000
VERSION     DAOQDO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18382_length_3673_cov_2.501658"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..209
                     /locus_tag="PKZ53_06740"
     tRNA            139..209
                     /locus_tag="PKZ53_06740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:169..171,aa:Thr,seq:cgt)
     gene            444..516
                     /locus_tag="PKZ53_06745"
     tRNA            444..516
                     /locus_tag="PKZ53_06745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Gln,seq:ttg)
     gene            737..2185
                     /locus_tag="PKZ53_06750"
     CDS             737..2185
                     /locus_tag="PKZ53_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40168.1"
                     /translation="MTTQFERDLRVEMLNSLLTTPHRELGAVATLHTELEQLDPLFYA
                     HLAVWYQQQGDVRDHKEVFVAHLLASDVAEYREAGFVLLQEFPPYEVARIVDYLKQIR
                     SKMPRSARTAVRQYLRFREDRPEMFDRAAIRGRKALKHLYATLRIKPSDRANAVLFKD
                     TPPEDSLAFVLKQLAKATEPADMARLIVEYRIPYTIAIGAIRQLTPAVLVALINSMTP
                     QEIINNLGALKNRGVMSNPEVKALVDGKLQKAADGNRVSALKAMKAADVTVLDQKTTG
                     HLEEIANRQDRKRGTIKRSTALLIDKSSSMDSAIEVGKQISALISGIATSDLFVYAFD
                     SMAYPIKAEGTDLSHWEKAFQRIFPGGNTSIGAPLQIMLNRKQVVEQIIIVTDEGENQ
                     SPYFAQVYDAYCKAFQVQPTVVIVKIGHVTNQLEQELKQAKANFETFTFAGDYYSLPN
                     LVPLLTRPSRLELLMEILETPLPRREALKAAS"
     gene            2296..3138
                     /locus_tag="PKZ53_06755"
     CDS             2296..3138
                     /locus_tag="PKZ53_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40169.1"
                     /translation="MYTTGLNSVDLCFVVDVTGSMGGFIQQAQKHLLDALNELTRLTQ
                     VDLHCGLVEYRDHPPQDNSFVTRVSALTGDFKRVESYIKKLSPQGGGDGPEAVYDGVV
                     AACTQIKWRPTSYRLVVLVGDAPPHGYPLPASDERQPRRQHLQRPVKMDGDAWPNGCP
                     CGSTIRSVTAEAEAKMITIHGLLMGGCVYARAAFSELATFTGGTCSTVQGSVIDVIVS
                     QLKQEFEGLAFDQQVLNVLVQSPLALIGEISEQLQAPRQRVARSVARLGKRGFLYQLA
                     MNVS"
     gene            3211..>3673
                     /locus_tag="PKZ53_06760"
     CDS             3211..>3673
                     /locus_tag="PKZ53_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40170.1"
                     /translation="MGQSTTLSQLIQSLLDADQALQTTRFVAPCVFRGKVQTRIQGLV
                     RTFSPVPKTFEGWGIFQPKNLNTADLIEEASLDIIARYCALQKSLRVRLITNLDRKTW
                     LAYPVNEADMQARFGSARPMMVHLVTEGSRFDVITARGNGQVWWYEDQDRRA"
BASE COUNT          929 a          904 c          945 g          895 t
ORIGIN      
        1 gagatgatca atcacaagtc agtagtcagt catcagtcgt caataaagca ttattttcaa
       61 caggttagcg atttacaact ccttttttgt tttttgaaaa ttgatttctg tcctgtactc
      121 cgacagaaga agcttacata cactcgctca gtggtagagc acctgcctcg taagcagaca
      181 ttcattggtt cgaatccaat gtgtagccaa aaacagcttt tgcaactcgt acaggacaga
      241 atctttaaaa acgaatgaag aatgaagaat gaagaatgaa gaaagtaata ccagtttgta
      301 gtcagcaatc agtcgtcagt cgttgactaa gttcatctga ttgaatcact tgactgtttt
      361 ctaatacgaa cacttcattg taaaactgta ttaccattca ggctcttttc tgtcctgcac
      421 tcaaaccgtt aaagcctaca tacactagct caactggtta gagcatctgt ttttggaaca
      481 gagggtagcg ggttcaaatc ccgcgtgtaa ccaccaattg cttttacaac ttgtgcagga
      541 cagaaccttt taaaccgaat gaagaattga gaatgaagaa aaaagaaagt tcctggtgcc
      601 tggtgcctgg tgcctggtgc ctggtgcttg gtgcttagtg cttggtgcct ggtgcttggg
      661 aaccaatact ttccaagaac caagaaccaa gcaccaagca cctgtatttt cttcatttgt
      721 aacggggttt caggatatga caacacaatt tgaacgcgat ctgcgggttg aaatgctcaa
      781 cagtttgttg accactcctc accgggaact gggggctgtg gcaaccctgc acaccgagct
      841 ggaacagctt gacccgttgt tttatgccca cctggctgtc tggtatcagc agcagggcga
      901 tgtgcgcgac cacaaagaag tcttcgtcgc ccatttgctg gccagcgacg tagccgaata
      961 ccgcgaagcc gggtttgtgc tcctccagga atttccgccc tatgaagtgg cgcgaattgt
     1021 ggattacttg aaacagattc gaagcaaaat gccccgttcg gcacggactg cggtgcggca
     1081 ataccttcgg tttcgtgaag atcgccctga aatgtttgac cgggcggcta ttcgtggacg
     1141 caaggcgctc aaacatttgt atgccacact tcggatcaag cccagtgacc gggccaacgc
     1201 ggttttgttc aaagacacac caccggaaga cagcctggcg tttgtcttaa agcaactggc
     1261 gaaagcaacc gaaccggcag acatggcacg actgatcgtt gagtaccgga tcccctacac
     1321 gattgcaatt ggggccattc ggcagttgac cccagccgtt ctggtggcgt tgatcaactc
     1381 gatgacgccg caggaaatca tcaacaacct cggggcattg aaaaatcgag gagtaatgtc
     1441 caaccccgaa gttaaagcac tggtggacgg taaactccaa aaggcagccg atggaaatcg
     1501 ggtttcagcc ttgaaagcga tgaaagcggc tgatgtcaca gttctggatc aaaaaaccac
     1561 cgggcacctt gaagaaatcg ccaaccggca ggatcgcaaa cgcggcacga tcaagcggtc
     1621 aaccgcgctg ttgattgata aaagcagcag catggattca gcgattgaag ttggaaagca
     1681 gatttcagcc ttgatttccg gaattgccac gtctgacctg tttgtatatg cctttgactc
     1741 aatggcctat ccaatcaagg cggaaggcac cgacctgtca cactgggaaa aagccttcca
     1801 gcgaattttt ccaggtggca acaccagcat tggggcgcca ttgcagatca tgctcaaccg
     1861 gaaacaggtg gttgaacaaa tcatcatcgt cacggacgaa ggtgaaaacc agtcacccta
     1921 ctttgcccag gtgtatgatg cctactgcaa agctttccag gtacagccaa cggtggtcat
     1981 cgtcaaaatt ggacacgtca ccaatcaact cgaacaggag ttgaagcagg caaaggccaa
     2041 ttttgagacg ttcacctttg cgggtgacta ttactcattg ccaaacctgg tgccgttgct
     2101 gacccgtcca tcacgacttg aactgctgat ggaaattttg gaaacgccgc tgcctcgacg
     2161 tgaagcgttg aaagctgcga gttaatggta gggcttgggg cttagggctt ggggcttttt
     2221 cgaagaactt cttcaaccct cagcctttag cccgataccc ccagcctgat accctcagcc
     2281 caaattggta tcactatgta cacaactgga ctcaattcgg tggatctttg ttttgtggtt
     2341 gacgtcacgg gcagcatggg tgggtttatc caacaggctc aaaagcattt gctggatgcc
     2401 ttgaacgaac tgaccagact gacccaggtt gatttgcact gtgggctggt tgaatatcgt
     2461 gaccatcctc cgcaggataa ctcgtttgta acacgagttt ctgcgctgac cggggatttc
     2521 aaacgggttg aaagttacat caaaaagctg tctccgcaag gcggtggcga tggtccggaa
     2581 gccgtgtacg acggagttgt agcggcctgt acccaaatca agtggcggcc aaccagttac
     2641 cgactggttg tgctcgtcgg agatgcacct ccgcatggat accccctgcc agcttcggat
     2701 gaacgacaac ctcggcgaca gcacctgcag cgtccagtca aaatggatgg tgatgcctgg
     2761 ccaaacgggt gtccctgtgg aagtaccatc cggtcagtga ctgccgaagc tgaggccaaa
     2821 atgatcacta ttcacggcct gttgatgggt ggctgtgtgt atgcccgggc tgccttttcc
     2881 gaactggcga ccttcacagg tggcacctgc tcaacggttc agggttcagt gattgacgtg
     2941 attgtcagcc agttgaagca agaatttgaa ggactggcgt ttgaccagca agttctaaat
     3001 gtactggttc aatcgccgct ggcactgatc ggagagattt ccgaacagtt acaagctccc
     3061 cggcaacgtg tcgctcgatc agttgcacga cttggcaagc gtggattctt gtaccagttg
     3121 gcaatgaacg tctcgtgaga gcaaatggct aaatcattaa aacagttgta tttccctgaa
     3181 ccctgaaccc tgaaccctca ttgatttttt atgggacaaa gcaccactct gagccagtta
     3241 attcaatctc tgcttgatgc ggatcaggcg ctgcaaacta cccggtttgt ggcgccctgt
     3301 gtgtttcgtg ggaaagttca aacccggatt cagggtctgg tgcgcacgtt ttccccagtg
     3361 ccaaagacgt ttgaaggctg ggggattttc cagccaaaaa acctcaatac tgctgatctg
     3421 atcgaagaag cttctcttga tatcattgct cgctattgtg ccttgcagaa atcacttcgg
     3481 gtgcggttga ttaccaatct ggatcggaaa acgtggctgg cctatcccgt caatgaggct
     3541 gatatgcagg cccggtttgg ttcagcccga ccgatgatgg tgcatctggt tactgaaggc
     3601 agccggtttg acgtcatcac cgcgcgtggc aatggacagg tctggtggta tgaagaccag
     3661 gatcgtcggg ccg
//
LOCUS       DAOQDO010000336         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18387_length_3672_cov_3.218966, whole genome shotgun sequence.
ACCESSION   DAOQDO010000336 DAOQDO010000000
VERSION     DAOQDO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18387_length_3672_cov_3.218966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PKZ53_06765"
     CDS             complement(<1..1515)
                     /locus_tag="PKZ53_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNJ40171.1"
                     /translation="MQSTKLLILRYRLFLVILVHSVIYFGAYFAAFCTQVEFQYSSEE
                     VGFPFWKTVWAVILVKLLVGVGFSGYRGWWKYVSLDDMLCIANQVTVGSLVLGGIQLL
                     VPDTFAVPISVLLIDWAFAFVALMTVRVSVRLFKERVQTQLAQFFHLSKATSVAGRAN
                     IVIVGAGDAGEALVREIQAHPELKLNVIGFLDDDPHKAGARIHHIPVLGTIREAEKIS
                     QRYDFQRFLIAVPSANQKQMRQIVRELSYTRLPYQTLPSFDQLVSGQVMVSQFREVQI
                     EDLLHRKPVQTDLASIGKLIRGRVVLVTGAGGSIGSELCRQIMKLEPACLVLAEQAEN
                     SLFYIEQELIRTFKDTAAHIVPAIVDITDRARLKEVFRQYQPEFVFHAAAHKHVPMME
                     CNPSEAVKNNIFGTKNVVDLSHAFGCQSFVMISTDKAVNPTSVMGTTKRAAELYIQGM
                     NRISATRFITVRFGNVLGSAGSVIPTFKDQIRRGGPVTVTHPEMNRYFMTIPEAV"
     gene            1932..>3672
                     /locus_tag="PKZ53_06770"
     CDS             1932..>3672
                     /locus_tag="PKZ53_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HNJ40172.1"
                     /translation="MSSNNSIESALTKLDSTNQTPVLATPDQLPARSDEIPAPMGWHN
                     PVISGFSAPAATPLTMRHIVQAISRYKGFILLVVVTALAVTAYGLSGTKSTYEATATL
                     QIDPEPAQMALSQEQSMAPSRVDPDYFNTQLKVIVSPEIVRTTIRAYQWDQNPQFPVT
                     GPTSVSSALLGHDQDPQAEAQSAPNQLVASAAASTPHQSEEQRPVSEALVKELSDHLR
                     VAPVPRTRLIKVTYQHTDPVLAAQIANSVAETYIKNNLNFRIRTSKNKSEFLQKRLAD
                     LQVDINDAETGMIAYGKMNEIVSLEPTQNTVAERLIMLNRQLIEAEGARIQVESDVLT
                     AKNTPPEMLPDIINHPGIQALRERLTTLNQKKAELLVEYTEDWPEVQQINQTIAQVQR
                     DLTTTQKNILDSIQSRYTASKEREEKLRGEYSRQMGLALLQNEKAVNYRIRQEEINTK
                     KELYKSLLTQAKEAEITASSEANNVSISSLATMPKDPVSPHVPLTLALVCIGSLVGAS
                     ALAVARSQFDNRIVTQEDIDQFVQMPTLGEIPSIDSFSPQTLSEKPIKVLTTGKSSLE
                     TEEEKKELAPARVQ"
BASE COUNT         1035 a          984 c          856 g          797 t
ORIGIN      
        1 gacagcctct ggaatcgtca taaagtaccg gttcatctca ggatgagtga ccgttactgg
       61 cccaccacga cggatctggt ctttaaacgt tgggatgaca ctgcctgcac tcccgagcac
      121 attgccaaaa cggaccgtaa taaatcgagt ggccgaaatt cgattcattc cctggatata
      181 gagttcagcc gcccgcttgg tggtgcccat cacactggtt ggattcacgg ctttgtcagt
      241 tgaaatcatg acgaaagact gacacccaaa cgcatgtgac aggtcaacaa catttttggt
      301 gccaaaaata ttgttcttca ccgcctcaga tgggttgcat tccatcatcg ggacatgttt
      361 gtgtgccgcg gcatgaaaca caaactctgg ctggtactgg cgaaatacct ctttgagccg
      421 ggcccgatcc gtaatatcaa caatggctgg aacaatatgc gcggcggtat ccttgaaagt
      481 gcggatcaat tcctgctcaa tataaaagag gctgttttct gcctgttcgg ccagaaccag
      541 gcaagctggt tccaacttca taatctgacg gcacaattca gaaccgatgc tgccaccggc
      601 accagtcacc agcaccaccc ggccccggat caatttccca attgaagcca ggtcagtttg
      661 aaccggtttt cggtgcagga ggtcttcaat ctgaacttcc cgaaactgtg acaccatcac
      721 ctggccactg acgagttgat caaaggaagg aagtgtctgg taggggagtc tggtatagct
      781 caactcacgg acaatttgtc gcatctgttt ttggttggca ctggggacag caatgagaaa
      841 ccgttggaaa tcatagcgtt gagaaatttt ttcagcttcc cggatggtgc cgagcacggg
      901 gatgtgatgg atacgggccc cagctttgtg ggggtcgtca tcgagaaacc cgatcacatt
      961 gagtttcaac tctgggtgag cctgaatttc ccggaccagg gcttcaccgg catcgccagc
     1021 accaacaatg acaatattgg cccggcctgc cacagatgtt gcctttgaaa ggtgaaagaa
     1081 ttgtgccagt tgcgtctgga cgcgttcttt aaacaatcgc accgacactc gcacagtcat
     1141 cagggccaca aaggcaaacg cccaatcaat cagtaacacg ctgatgggaa ccgcaaaggt
     1201 gtcaggcacc agtaactgga ttccacccaa caccagagac ccaacggtga cctgattggc
     1261 aatacacaac atatcgtcca aactgacata tttccaccag ccacgatatc cggaaaatcc
     1321 gactccaacc agcaatttca ccaggatgac cgcccatact gttttccaaa acgggaaccc
     1381 gacctcttca gagctgtatt gaaattcaac ctgcgtacaa aaggcagcaa agtaagctcc
     1441 gaaataaatc accgagtgaa ccaaaatgac cagaaatagt cggtaccgga gaataaggag
     1501 ttttgtggat tgcatagtga ttcaaccttt attcgaaaat atgaagggac ggcatctcag
     1561 ccggtgtttc caagttagat atccctttcc agttcacttt gtggcgctgc aaatcaaatg
     1621 ccacttgcag ttttttttat tttcttgggc tgaattttga atcatttatg aaataatcct
     1681 gcgccagaag ggcggaaaat gaaaaccgaa gaaaaattta cctgaccttt cgatgcccaa
     1741 accaggaatg gtggaacacc atttgcactt tcccctggca gacagacaaa gtcagttttt
     1801 tgccagacct gaacccaggg tcccccacac cacagtcaaa accggggtct ggaaaccatc
     1861 cagggtctag ccagaggttg ctttgtatca ctgttggcac ctaaataatg accagatttt
     1921 tggaggcggc tatgtcatca aataactcga ttgaatcagc tttgacgaaa ttagactcaa
     1981 ccaaccagac accggtactg gcaactcccg atcaacttcc agccagaagc gacgaaatcc
     2041 cggcaccgat gggttggcat aatcctgtca tatctggttt ttcggcacca gcggcgactc
     2101 cgttgacgat gaggcacatc gtacaggcaa tctcacgtta taaaggattc attttgctgg
     2161 tggttgtaac agcacttgcc gtcaccgcct atggcctgtc agggactaag tccacctatg
     2221 aagcaaccgc gacgctccaa attgaccccg agccagcgca aatggccctg agtcaggaac
     2281 aatccatggc tccaagccgg gttgatccag actatttcaa tacccagtta aaagtgattg
     2341 tcagcccgga gattgtgcga accacgatcc gggcatatca gtgggaccaa aacccgcaat
     2401 ttccagttac ggggcccact tcggtttcaa gtgccctgct tggccacgac caggacccac
     2461 aggctgaagc gcaatcagcc cccaatcaac tggttgcctc agcggcggct tcgaccccac
     2521 accaatcaga agaacaacgg cccgtgagcg aggccctggt taaagaatta agcgaccatc
     2581 tacgcgtggc accagtgccc cgcacccggt taattaaagt gacctatcag cacacagatc
     2641 cggtactggc cgcccagatt gccaacagtg tggctgaaac ctatatcaaa aataatctga
     2701 attttcggat tcgaacctca aaaaataaat ctgaattttt gcaaaaacgg ctggctgatc
     2761 tccaggttga tatcaatgat gcggaaaccg ggatgattgc ctatggcaaa atgaatgaaa
     2821 ttgtctcgct ggagccaact caaaatacgg tggccgagcg cctgattatg ctcaatcgcc
     2881 agttgatcga agccgaaggg gcccgcattc aggtggaatc agacgtactc acggcgaaaa
     2941 acacaccgcc cgagatgctc cctgacatca tcaaccatcc tggcatccag gccctgcgcg
     3001 agcgcctgac cacactcaat caaaagaaag ctgaattact ggtcgaatac accgaagact
     3061 ggccagaagt ccaacagatc aaccagacca ttgcccaggt tcagcgcgat ctcaccacaa
     3121 ctcaaaaaaa tatcctggat tcaatccaga gccgctatac agcctcgaaa gaacgtgaag
     3181 agaagctgcg cggtgaatat agccggcaaa tgggcctggc actgttacag aatgaaaagg
     3241 ccgtcaacta tcgaattcga caggaagaaa tcaacaccaa aaaagaactc tataaatcgc
     3301 tcctgaccca ggccaaagaa gctgaaatta ctgccagttc agaagccaac aacgtgtcca
     3361 tttcgagcct ggccaccatg ccaaaagatc cagttagccc gcatgtgcca ttgaccctgg
     3421 cgctggtttg tatcgggtcc ctggttggtg cctcagcact ggccgtggct cggtcccaat
     3481 ttgataatcg gatcgtcacc caggaagaca ttgaccagtt tgttcaaatg ccaaccctgg
     3541 gcgaaatccc cagcattgat agtttttcac cacaaacgct gtctgaaaaa ccaatcaaag
     3601 ttttgacaac cgggaaatcc agccttgaga ccgaggaaga aaagaaggaa ctggcaccgg
     3661 ccagagttca gc
//
LOCUS       DAOQDO010000337         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18395_length_3671_cov_2.775166, whole genome shotgun sequence.
ACCESSION   DAOQDO010000337 DAOQDO010000000
VERSION     DAOQDO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18395_length_3671_cov_2.775166"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKZ53_06775"
     CDS             complement(<1..319)
                     /locus_tag="PKZ53_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40173.1"
                     /translation="MGSEKLLVKKEFLPTRCEICHQADQFDPVRNICQRCQSLAVARN
                     PQPVALAELQSMATPRPVRIQNSIEQSAIHISQGLAFVVLGLLVLFAKSSPLLIFVAL
                     SGVW"
     gene            complement(351..530)
                     /locus_tag="PKZ53_06780"
     CDS             complement(351..530)
                     /locus_tag="PKZ53_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40174.1"
                     /translation="MTPLSYSAACDLCPEQVGTGRVLECGGLTPLSYSAACDLCPEQV
                     GTGRVSPGDDILGDM"
     gene            660..803
                     /locus_tag="PKZ53_06785"
     CDS             660..803
                     /locus_tag="PKZ53_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40175.1"
                     /translation="MNSFAHCVAVPATQPMCALSIVSRDAVSISIIAISIIAIRTQGV
                     EAT"
     gene            893..1921
                     /locus_tag="PKZ53_06790"
     CDS             893..1921
                     /locus_tag="PKZ53_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HNJ40176.1"
                     /translation="MSVATVPSTESEVASHATAAWPISFQDVVNASRQLRQYLPVTPL
                     RNYQALDQAVGHNIQVHVKHENHLPTNSFKVRNGLSVLASLTPEQRKHGVVAATRGNH
                     GQGVAWAGALLGIPVTICVPLSNNPEKNAAMRGFGATVIEDGHDYDEAIRVAQELVHS
                     RAMTLVHATNNRRVLAGAATMSLEMVVDQAAELDAVVMAIGGGSMAVGALTVLRKLRP
                     EIEVYGVQAEGASAMHESWHAGQPVVKHTAQTIADGLATRGVYEMTFSTLREGLTDFI
                     TVSDELIAEAVRMLLKTTHNLAEGAGAAGLAGLLSLRERLAGKRVGIVLSGSNIDETT
                     LRWVMKAA"
     gene            2187..>3671
                     /locus_tag="PKZ53_06795"
     CDS             2187..>3671
                     /locus_tag="PKZ53_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNJ40177.1"
                     /translation="MWNQEKSSCGVGFVASLHQVSNHENLQQALHALRCVEHRGACGA
                     DLETSDGAGVMTDIPFELLGFEKHSVGVAQLFLPQDEPYRKEILSIFEDTFSFLDVEL
                     IGYREVPINAEVLGPQARQTMPVLVQAFLRRPVHCTTVDSFNKLLYIGKQHARTRVIR
                     RFSNYEVFFSSISAETIVYKALVRSEKLDQFYLDLQSPQYRTRFALFHRRFSTNTSTA
                     WDKAQPFRLIAHNGEINTIAGNRSWAYSREKTLGFQANELITHHGISDSGSLNEMVEA
                     LRYRSHLANINDILAIMMPPADRQNDYYTFWARTMEPWDGPAIILFSDGNLVGARLDR
                     NGFRPGRWMLTEGHFYVCSEAGAFALNESDIRLKGALHAGNAVTVNLRTGLVSFEDPS
                     TIAGYQGVRFDPRLVPLPTTTQVQPAARADKTVFGYTEEDSNRILLPMMATGKEPIGS
                     MGDTARLAVLSDEPRSFFDFFYQDFAQVTNPPVDYLRETMATDLT"
BASE COUNT          883 a         1008 c          963 g          817 t
ORIGIN      
        1 accacacacc agacagcgca acaaaaatca gtagcgggga acttttggca aagagaacca
       61 ggaggcccaa aaccacaaag gccaacccct gggaaatatg gatggcactt tgttcaatac
      121 tattctggat tcggactggc cggggcgtag ccatagactg aagttcagca agcgcgacgg
      181 gttgaggatt gcgcgcgacg gcaagtgact gacagcgctg acaaatgttt cgcaccgggt
      241 caaactggtc ggcctgatga cagatttcac agcgcgttgg aagaaattct tttttgacaa
      301 gtaatttttc ggaccccata gagcacacca ggtattgaag tatgaaggat ctacatgtca
      361 cccaaaatat catcaccagg cgacacacga ccagtaccaa cctgttcggg acacaaatca
      421 caagccgccg aatatgaaag cggtgtcaag ccaccgcact ccaaaacacg accagtacca
      481 acctgttcgg gacacaaatc acaagccgcc gaatatgaaa gcggtgtcaa gccaccgcac
      541 tccaaaaggg gcttgtcaca agcaaacatt ctcaggcaga atggggttgt cattctctcc
      601 cccttacaat ttcagatttc aggtaccagc gcttgaacgc acatacttgc ctcacgccat
      661 tgaattcgtt tgctcactgc gttgccgtcc cggcgactca gccgatgtgt gccctgtcta
      721 tagtaagtcg tgacgctgta tctatttcga ttattgccat ttcaattatt gccattcgca
      781 ctcaaggcgt ggaggctacc tgacacccaa ctgtacaagt tgaaagtgat acccaaagcc
      841 accacgccag accaggcggg tggctttttt gattttcgag gagaaaacca caatgtctgt
      901 cgccaccgtt ccatcaaccg aatctgaggt agccagtcac gcaactgcgg cctggccaat
      961 ctcctttcag gatgtggtca acgcatcgcg tcaactccgg caatatctgc cagtgacgcc
     1021 gctccggaat tatcaggctc tggatcaggc cgtgggccac aatattcagg tccatgtgaa
     1081 gcacgaaaac cacctgccaa ccaattcttt caaagttcgc aatgggttaa gtgtgctggc
     1141 ttcgctgacg cccgaacaac gcaagcacgg tgtggtcgcc gccacgcgtg gaaatcatgg
     1201 tcagggcgtc gcatgggctg gcgcattgct gggaatcccg gttacaatct gcgtccccct
     1261 ctcaaacaat cctgaaaaaa acgcagctat gcgtggcttt ggagcaacgg tgatcgaaga
     1321 cggtcacgat tatgacgagg ccattcgtgt cgctcaggag ctggttcact cgcgcgcaat
     1381 gaccctggtt cacgccacca acaaccgacg ggtgctggcc ggtgccgcca ccatgtcgct
     1441 tgaaatggtg gtggatcagg cggctgaact cgatgccgtg gtcatggcca tcggcggcgg
     1501 gtcaatggcg gtcggcgcct tgacggtgct gcgcaaactg cgcccggaga ttgaagtcta
     1561 cggtgtccag gcagaaggcg catcggccat gcacgaatcg tggcacgccg ggcaaccagt
     1621 ggtcaaacat accgctcaga cgattgccga tggcctggcc acccgtggcg tctatgaaat
     1681 gaccttcagt accctccgcg aaggactcac cgatttcatc acggttagcg acgaattgat
     1741 cgccgaagcc gtccgcatgc tgcttaaaac aactcataat ctcgctgaag gcgctggcgc
     1801 ggctgggctg gccggattac tgtcgctccg cgaacgactg gctggcaaac gggttggaat
     1861 cgttctctct ggatcaaaca ttgacgaaac aaccctccgc tgggtgatga aagccgctta
     1921 agaccggggc tgaagacttc gggctgaaga cttcgggctg aagacttcgg gctgaagaag
     1981 acgggcttgg ggctgaagaa gtcgggctga agacatcgag ctgaagaatt gggggttatt
     2041 tcatccctca tccttcatcc ctcatccctt caattgctcc gagcttgcga gtcttcaacc
     2101 ccaagcccga aatcttcaac cccaagccct gagcccgatg tcttcagccg caggattttc
     2161 agccctgccc catgaaagga ctcactatgt ggaatcaaga aaaatcaagt tgtggggttg
     2221 gttttgtggc cagtctccat caggtcagca accacgaaaa cctgcaacag gcactccatg
     2281 cgctccggtg cgttgaacat cgcggggcct gcggcgccga tcttgaaacc agtgacggag
     2341 ccggggtaat gaccgatatt ccctttgaat tgctggggtt tgagaaacat tctgtcggcg
     2401 tggcgcaatt gtttttgccg caggatgaac cctatcgaaa agaaattctg tcgatttttg
     2461 aagacacttt ttcttttttg gatgttgaat tgattgggta tcgcgaagta ccaatcaatg
     2521 ctgaagtact tggaccgcag gcacgacaga cgatgccagt gctcgttcag gcatttttgc
     2581 ggcggccagt tcattgcacc accgtggatt cattcaacaa gctgctctat atcgggaaac
     2641 aacacgcccg caccagagtg attcgccggt tttcgaatta cgaggtgttt ttttcgtcaa
     2701 tttctgcgga aaccattgtg tataaagcgc ttgtgcgatc agaaaaactt gatcaatttt
     2761 acctggattt gcaatctccc cagtatcgca cccggtttgc cctctttcat cgccgattca
     2821 gcaccaacac cagcaccgcc tgggataaag cccagccttt tcgcctgatt gcccacaatg
     2881 gcgaaatcaa cacgattgct ggcaaccggt cgtgggctta ctcgcgggaa aaaacgctcg
     2941 gctttcaggc caacgaactg attacccacc acggaatcag cgatagcggc agtctcaatg
     3001 aaatggtcga agccctgcgg tatcgcagcc atctggctaa tatcaatgac attctggcca
     3061 ttatgatgcc accggcagac cgccaaaatg actattacac gttttgggcg cgcacgatgg
     3121 aaccgtggga tgggccggcg attattctgt tttcagacgg caatctggtc ggtgcccgac
     3181 ttgaccgcaa tggattccgg cctggtcgct ggatgctcac cgaaggccat ttctatgtgt
     3241 gctctgaagc tggcgccttt gcgctcaacg aaagcgatat ccggttgaaa ggcgcacttc
     3301 atgctggcaa tgccgtgacg gtcaatttac gaactggact ggtgagtttt gaagatccaa
     3361 gcacgattgc cggctatcaa ggggtgcggt ttgatccacg cctggtgcca ctaccgacca
     3421 cgacgcaggt gcaaccagcc gccagggctg acaaaacggt ttttgggtac accgaggaag
     3481 attcaaaccg catcctgctg ccgatgatgg ccaccggcaa ggagccaatt gggtcaatgg
     3541 gtgacacggc ccggctggcc gtgctttctg acgaaccacg ttcatttttt gactttttct
     3601 atcaggattt tgcccaggtg accaatccgc cggttgatta cctgcgcgaa acgatggcca
     3661 ccgatttgac g
//
LOCUS       DAOQDO010000338         3669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18415_length_3669_cov_3.269231, whole genome shotgun sequence.
ACCESSION   DAOQDO010000338 DAOQDO010000000
VERSION     DAOQDO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18415_length_3669_cov_3.269231"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PKZ53_06800"
     CDS             complement(<1..1433)
                     /locus_tag="PKZ53_06800"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40178.1"
                     /translation="MRFFLRRSAPVLAILVWTLWWTLPVGAVPVSSKQLAIHYQVTLE
                     KAGNKSILVEIRLQGLDRPAIDQAIPAWTPGSYEILNYAQYLSELTARGSSGQALETR
                     FVDKQTWRIQCAGQAQVSVRYRVQCDQLTVDSNWLGPHYGQLQGAATFLYLVGEKEIP
                     RTVEFVLPPGWNVAMPLVEAGGKTFTARDYEALIDAPIVLGKYRRTDFMVQKIPFSLF
                     TVRESADIEGLQEAITKIALVYAEMMGSFPFERYFLIYIPFESNSFSVIEGLEHANST
                     LINFSPESLVDYPLASVDFLSVTSHEIFHAWNVKRLRPKELVGASLAHEVYTPSLWFA
                     EGVTDYYAMLGLVRGKLIRLQEYAAYLERNLKALEEAPGRQFMSLEDASLATWLTGTS
                     NFQNIQLDYYGKGAIVAALLDIELRTRTQGRQSLDDLVRAMYTRFPETSPGYTNDDIL
                     QELNRLTGTDFTEFFRRNIKSSDELLYR"
     gene            complement(1608..2234)
                     /locus_tag="PKZ53_06805"
     CDS             complement(1608..2234)
                     /locus_tag="PKZ53_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40179.1"
                     /translation="MNKLRGILAIGCVVAMLGLSGSLLPVWSAVPKKRQYRTGQSVRP
                     GDVLRLELLSPIGTKDSREGDQFTARVLSPNTWQDAFIEGHVIRINRSGRIAGRAEVE
                     LSFDTLVMADQREQQISAEVESVERNEVEVDAEGRVKSRGSQTRDAILIAGGAGIGTA
                     IGAIIGGKKGAAIGSAIGAAAGTTTVLTTRGEDLDFPAGTTMQIRITQ"
     gene            complement(2370..>3669)
                     /locus_tag="PKZ53_06810"
     CDS             complement(2370..>3669)
                     /locus_tag="PKZ53_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ40180.1"
                     /translation="KGNIRIERYWDAIVETPQQELTEAEIIPRLRDLLTQSIEKRMMS
                     DVPFGVFLSGGVDSSANVALMSACSTEPVRTFTIGFKGIETYNELEYARQIARHFGTD
                     HHEILIGPDEVLSFLPELIFHQDEPIADPVCVPLYYVSKLVRESGTIVVQVGEGSDEI
                     FCGYPNYVDFLKIYERGWSHLERLPRGVRSGLAAAAAPFSQFAAETFMPRGKKLIPDL
                     VRRLGANEELFWGGTVVYDEVSKSRLLSKELRDRLGHISSHDVVRGLVGHFDSKKPDG
                     DFLERMIYLDLNLRLAELLLMRVDKITMATSIEARVPFLDHQFVEFAMTIPRHLKYKD
                     GQTKYILKKALEGVLPDNIIYRKKQGFGLPIKEWFMDGFGQGLDQRILNSRLRKRNYF
                     NYSEIEHMLAAHKAGKIDYSFNLWGLLNLSLWYERWIEPA"
BASE COUNT          986 a          958 c          848 g          877 t
ORIGIN      
        1 cgatatagca actcatcact gcttttaatg ttccgccgaa aaaactcggt aaaatcggtt
       61 ccggtcaatc ggttcagttc ctgcaaaata tcatcatttg tatatccagg actcgtctca
      121 ggaaaccggg tatacatcgc ccggacgaga tcatcgagtg actgtcgccc ctgggttctg
      181 gttcgaagtt cgatatcaag caaggcagcc acaattgccc ccttgccata gtaatcaagt
      241 tgaatgttct gaaaattgct ggtgccagtc aaccaggttg ccaatgaggc atcttccagg
      301 ctcataaatt gccgtcccgg agcttcttcc agggctttca gattgcgttc gagataggcg
      361 gcatactcct gcagtcgaat gagttttccc cgtaccaggc caagcattgc ataatagtca
      421 gttacccctt cagcaaacca taaggatggc gtatagactt catgggcgag tgaagcccca
      481 accagttcct taggccgcaa ccgtttcaca ttccaggcat ggaaaatttc atgggatgtg
      541 acggaaagaa aatcaacgct ggccagcgga taatcaacca gtgattcagg tgaaaaattg
      601 atgagcgtcg aatttgcatg ctccagccct tcaatcacac tgaacgagtt ggattcaaag
      661 ggaatataaa tcaaaaaata ccgctcaaag ggaaagcttc ccatcatttc ggcatacacc
      721 agtgctattt tggtgatcgc ctcctgtaac ccctcaatat cggcgctttc ccgaacagta
      781 aataaagaaa acgggatttt ttggaccatg aaatcagttc gccggtattt tccaagcacg
      841 attggagcat caatcagcgc ttcataatct ctggcggtaa aggtttttcc tccagcttca
      901 accagcggca tggcgacgtt ccatccaggg ggcaagacaa attcaacagt gcgcggaatt
      961 tctttttccc caaccagata gaggaatgtc gcggcacctt gaagctggcc atagtgcgga
     1021 cctaaccagt tggaatcaac cgttaattga tcacactgca cacgatagcg caccgaaacc
     1081 tgggcctgcc cggcacattg aattcgccac gtttgcttat caacaaagcg ggtttccagg
     1141 gcctgaccag acgatcctct ggcagtcagc tctgaaagat actgggcata gttcagaatt
     1201 tcataagaac ctggggtcca ggctggaata gcctggtcta tcgctggacg gtcaaggccc
     1261 tgaaggcgta tttcaaccag aattgacttg ttccccgctt tttcaagggt gacctgataa
     1321 tgaatagcga gttgtttgga agaaaccggc acggcaccga ctggcagggt ccaccagaga
     1381 gtccaaacca aaatagccaa caccggagct gaacggcgga gaaaaaatcg cataaagtaa
     1441 ttgatagatt cgaaggagtt agcagcaagc tcagtatatg ttttttctgg taaagaacaa
     1501 gctatgaaat caaccgcacc tgtatttggc aggctgaaaa aagaaaagcc gagccattta
     1561 tttctggccc ggcttcattc ctttataagt gtgcgataga agaattctca ctgagtaatg
     1621 cggatttgca tggtggtacc ggctggaaaa tcaaggtctt cgccacgggt cgtcaagaca
     1681 gtggttgttc cggcggcggc accaatcgca gagccaatcg ccgccccttt cttcccacca
     1741 atgatggcgc caattgcggt gccaatcccg gccccgccag caatcaaaat ggcatcgcgg
     1801 gtctgtgacc cacggctttt gacacggcct tcagcatcca cttcaacttc attgcgttcg
     1861 actgattcaa cctcggctga gatttgctgc tcacgttgat cagccattac cagcgtgtca
     1921 aaggaaagct cgacctcagc ccgtccggca atccgcccac tacggttaat tcgaatgacg
     1981 tggccttcaa taaaagcatc ttgccatgtg ttgggcgaga ggacccgggc agtaaattga
     2041 tcgccttcgc ggctgtcctt ggtgccaatc ggtgacaaaa gttcaaggcg cagcacatcc
     2101 ccaggacgca cagactgacc tgtccgatac tgtcgttttt tcggtactgc cgaccagacc
     2161 gggagcaaac tcccagataa acccaacatt gcaaccacac agccgattgc cagaataccc
     2221 cgcagcttgt tcataaacgt ctcctgcgaa caaaacctga aacccagacg caattgtttt
     2281 gattgaggcg gatgagagaa atggccgctc gtgtagatgt ctggacgatg agttgggatg
     2341 tctggatttt ttcgcctctt tggtcgaaat cacgccggct caatccagcg ctcataccag
     2401 agactcaagt tgagcaatcc ccatagatta aaactatagt caattttgcc ggctttatgg
     2461 gcggccagca tatgttcaat ctcactataa ttgaaatagt ttcgctttcg cagacgagag
     2521 ttcagaatcc gttgatcaag tccctgacca aacccgtcca taaaccattc tttgatagga
     2581 agaccaaacc cctgtttttt tcgatagata atgttgtcgg gcaggactcc ttcaagtgct
     2641 tttttcagaa tgtatttcgt ctgaccatct ttgtatttga gatgacgagg aatcgtcatg
     2701 gcaaattcaa caaactggtg gtccagaaat ggcacccggg cttcaattga tgtcgccatg
     2761 gtaattttgt caacccgcat cagtagcaat tcagccagcc gtagattgag gtcaaggtag
     2821 atcatgcgct ccagaaaatc accatccggt ttttttgagt caaaatgacc aaccaacccg
     2881 cgcacaacat catgagaaga gatatgacct aaccggtcac gcaattcttt ggaaagcagg
     2941 cgtgacttac tgacttcgtc atacaccact gtgccacccc aaaacagttc ttcattggcc
     3001 cctaagcgtc ggaccagatc gggaatcaat ttttttcctc gtggcataaa tgtttcagcc
     3061 gcaaactggc taaagggcgc agccgcagcg gccagaccag atctcacccc acgcggtaac
     3121 cgctccaggt gcgaccatcc ccgctcgtag atcttgagaa aatcaacata attaggatag
     3181 ccacaaaaga tctcgtccga cccctcaccc acctgaacca caatcgtgcc gctctcacgg
     3241 accagctttg agacatagta cagcgggaca cagaccggat ccgcaatggg ctcatcctga
     3301 tgaaaaatca gttctggaag aaatgacagc acttcgtctg gaccaattag aatttcatgg
     3361 tggtcagtac caaagtggcg ggcaatctgg cgggcatatt caagctcgtt gtaggtctca
     3421 attcctttaa agccaatcgt aaacgtacgc actggctcgg tcgagcaggc tgacatcaaa
     3481 gcaacattgg cgcttgaatc cacccctcca cttaaaaaga cgccaaatgg aacgtctgac
     3541 atcatccgct tttcaattga ttgggtgagc aggtcacgca aacgaggaat aatttcggct
     3601 tcggtgagtt cttgctgtgg cgtttcaaca attgcatccc agtaacgctc aatccggatg
     3661 tttcccttt
//
LOCUS       DAOQDO010000339         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18559_length_3651_cov_3.056730, whole genome shotgun sequence.
ACCESSION   DAOQDO010000339 DAOQDO010000000
VERSION     DAOQDO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18559_length_3651_cov_3.056730"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="PKZ53_06815"
     CDS             complement(<1..1557)
                     /locus_tag="PKZ53_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNJ40181.1"
                     /translation="MPTNEKQTLSSIREELNSKQGRQFWRSIEELTESEEFLELIRNQ
                     YPRQASLLGDAIDRRRFLQIMGASFALGGLTACNPIAAPTDKIVPYVRAPEEILPGKP
                     LYYATSMVLGGFASGILVETHMGRPTKVEGNPDHPASLGATSAFAQAAVLDLYDPDRS
                     KNVLYYGQPRPFDVFLRDVRELLAASKARQGAGIRFLSETVTSPTLTAQFKALFEAYP
                     QAKWYQYDAVNRDNYYEGTRLAFGSYAHPVYHFDKADVVVSLDANFLGQGPGVVRYTK
                     DFSRRHRVRKDKKEMSRLYVAETMPSVTGTVADHRMAAKPSEILGLAKRIAAGVGVPV
                     KDAPAGGANDKWVTALIEDLKKNKGKSVVLAGDNQPPAVHALVHAINQSLENNGKTVT
                     FTEPLEASLPNQPSPHKQVESLKQLVQELDGGQVELLIILGGNPAYTTPVDLQFAEKV
                     KKAKTAVHLSSHYDETSVNCHWHIPQSHFLETWTDACAFDGTVSVVQPLIEPLYKESK
                     SAHELLVAFSD"
     gene            complement(1582..2235)
                     /locus_tag="PKZ53_06820"
     CDS             complement(1582..2235)
                     /locus_tag="PKZ53_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ40182.1"
                     /translation="MSQVFHRSMNSVAKISIVGGALLAATLLLTLDLAIRSPLVTNQE
                     VAQPQPVQFSHKHHYSGLGIHCLYCHTSVEDSSFANVPPTATCMNCHSQVWYTAPILQ
                     PVRTSFKENQALEWNRVHNLADFAYFNHSIHVNKGIGCVSCHGRIDQMQLAYKAQPMT
                     MEWCISCHKNPEPNLRPRDQIYNFEYKRTDASEGPKLMKEYKIRSARMLTSCSTCHR"
     gene            complement(2926..>3651)
                     /locus_tag="PKZ53_06825"
     CDS             complement(2926..>3651)
                     /locus_tag="PKZ53_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ40183.1"
                     /translation="VNDVPVEKLDGFSGEVGETLRFKFLDQTDNPRDISLNVKYLATT
                     PIQTARLLEKNVLYVRFDRFEENTGKWFQAVLAQNEMASGLIVDLRRNLGGLVSTVRQ
                     CLEVLSAEDVAVGEFIERSGKEKIFRISGKGKRAWNKPVVVLIDKYSASGSEILAAAL
                     QEAGRGKVIGRPSAGAVLASIKKDLPDGGELQYSIRDFLTVRKARLEGHGVKPDEVIE
                     LSVNAVRQKSDQDVARALEILGQ"
BASE COUNT          848 a          974 c          949 g          880 t
ORIGIN      
        1 gtctgaaaat gcaaccagca gttcatgagc cgatttggat tccttataga gcggttcaat
       61 caatggctga accactgata ccgtcccgtc aaaagcacag gcatcagtcc aggtttccag
      121 aaaatggctt tgcggaatgt gccagtgaca gttgaccgat gtctcgtcat agtgcgaact
      181 caggtgcacc gccgttttgg cttttttcac cttctcggca aattgcagat caacgggtgt
      241 tgtataagcc gggttgccgc caagaataat gagcaattca acctgtccgc catcgagttc
      301 ctgtaccagt tgtttgagcg attcaacctg cttgtgcggt gatggctggt ttggcagcga
      361 ggcttccagc ggctcggtaa aggtcaccgt tttgccgttg ttttccagcg actgattgat
      421 ggcatgcacg agggcatgca cggctggggg ctggttatcg cccgccagaa ccacgctctt
      481 gcctttgttt ttcttgaggt cttcgatcag ggcagtcacc catttgtcgt ttgccccacc
      541 cgccggagca tctttgaccg gcaccccaac ccctgccgcg atgcgtttgg ccaaccccag
      601 gatttcgctt ggtttggcgg ccatccggtg atcagcaaca gttccggtga cgctcggcat
      661 cgtctcagcg acgtaaagtc gcgacatctc tttcttgtct ttgcgaaccc ggtgacggcg
      721 tgagaaatct ttcgtgtagc gcaccacgcc cggtccctgt cccaggaaat tggcgtcgag
      781 cgaaacgacc acatccgctt tgtcaaagtg gtacaccgga tgcgcatagc tgccgaaagc
      841 cagccgcgtg ccttcatagt agttgtcacg gttcacggca tcatattgat accatttggc
      901 ttgtggatag gcttcgaaca gggccttgaa ctgggccgtg agcgttggcg aggttaccgt
      961 ttcagacagg aagcggatac cagccccctg tctggctttg ctggctgcca gcaactcgcg
     1021 cacgtctctg agaaagacat caaagggacg cggctggcca taatagagga cgtttttgga
     1081 acggtcggga tcatacagat caagcacggc ggcctgggca aaggcactgg tcgcccccag
     1141 actcgccggg tgatcggggt tgccttcaac tttggttggc cggcccatgt gggtttcaac
     1201 cagaatcccg gatgcaaatc cacccagcac cattgatgtc gcgtagtaga gcggtttccc
     1261 tggcaggatt tcctctggag cgcggacata cggcacaatc ttatccgtcg gagcagcgat
     1321 tggattacag gcggtcaacc cgcccagcgc aaacgacgcc cccataattt gcaggaagcg
     1381 gcggcggtca atcgcatcac ccaacagtga cgcctgacgg gggtattgat tgcggatcag
     1441 ctcaagaaat tcttcgcttt cggtgagttc ttcaatgctt ctccagaact ggcggccttg
     1501 cttcgaattc agttcttctc gaatcgagga cagggtctgt ttctcattgg taggcatagt
     1561 gttttcggtt cccacaattc cttatcggtg gcacgttgag caactcgtca gcatgcgtgc
     1621 gctgcgaatc ttgtattcct tcatcaactt cggaccttca gaggcgtcgg tgcgcttgta
     1681 ctcaaagttg taaatctggt cgcgcggacg aaggtttggc tcagggtttt tatggcagct
     1741 aatacaccac tccatcgtca tcggctgcgc cttataggcc agttgcatct ggtcaatccg
     1801 cccgtggcac gacacgcacc caatcccttt attgacgtga atgctgtggt tgaaataggc
     1861 aaaatcggcc aggttgtgaa cccgattcca ttccagcgcc tgattttctt taaagctggt
     1921 gcgaaccggt tgcagaattg gggccgtgta ccagacctgg gagtggcagt tcatgcaagt
     1981 ggccgtaggt ggcacattgg caaacgagga atcttccacc gatgtgtggc agtacaggca
     2041 gtgaatgcca agcccggagt agtggtgttt gtggctgaat tgcacgggtt gtggttgagc
     2101 gacctcctgg ttggtaacca gcggagagcg aatggccaaa tccagtgtca gcagcaatgt
     2161 ggccgccagc agtgcgccac cgacgatact aatctttgcc accgagttca tactgcggtg
     2221 aaagacctgt gacataaaat ccctctcatt ccaatcgagc ttgcatccgg gcgatagtca
     2281 actcatcaga cgcacatcca cttacagcaa ggtaaggggc agcgtgggag ccgaccagtg
     2341 acacaagaag caaaactcgg aactaagata gctagccttg gcagttcctg gcgtgatgtc
     2401 cgccgtgagc agttggtgct gagaaaagcg cgtagtgttg tgaatacaga gcaaggaagg
     2461 cgcttttata caatttttca atcagagtca acccacacag cggctcaccg tcgaaaaaac
     2521 ttaggtgatg ggggcagaag tcggtcgaaa tacaagttat gaggaatgac ggacaaaaaa
     2581 tgagggaact attttttttt caacaaaata gcttcttcat aactcataat tcaacgaatt
     2641 ttggggacct gtagaaagta tgtagagaag acattgggca gtgaacttct gcggatgccg
     2701 cgcattgtgc cacagcttta gtgacttgaa aagtgttgac tttgccacaa attgcggggt
     2761 tgcagggtca agaaccataa aatttgcaac ccaggcagtt ccccaaacct gatgtaactc
     2821 tttgaaaaaa aagcagattt tccgatgagg actggtcttc gcagatgcga gaaaaaaagt
     2881 aatcaagaaa ctgaaaacac cttacattat cagaccgaaa accgatcatt gccccaggat
     2941 ttccagcgct cgggccacat cctgatcact cttttgacgc actgcgttga cgcttaactc
     3001 aatgacttca tccggtttga ccccgtgacc ttctaaccgg gctttgcgta cagtcagaaa
     3061 atcccggata ctgtattgca actcaccccc atcggggaga tcttttttaa tcgaagccag
     3121 caccgccccg gcacttggcc gaccaatcac cttgccccgc ccggcttcct gcagggctgc
     3181 cgccaaaatc tcagagccgc tggcactata tttgtcaatt aacacgacca ccggcttgtt
     3241 ccaggcccgt tttcccttac ctgaaatccg gaaaattttt tcttttccgc tgcgctcgat
     3301 aaattcaccc acagcgacat cttcggcaga aagcacctcc aggcactggc gtacagtcga
     3361 aaccagcccg cccaggttgc ggcgaagatc cacaatcaac ccgctcgcca tttcgttttg
     3421 cgccagcacg gcttgaaacc atttgccggt gttttcctca aaccgatcaa agcggacata
     3481 aagcacattt ttttccagca gccgggctgt ttggattggc gtggtcgcca aatatttgac
     3541 attcaatgag atatcgcgtg ggttatcggt ctggtccagg aatttgaacc gcaaggtctc
     3601 gcccacttca cctgaaaagc cgtccagttt ttccaccggc acatcgttga c
//
LOCUS       DAOQDO010000340         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18681_length_3638_cov_2.901758, whole genome shotgun sequence.
ACCESSION   DAOQDO010000340 DAOQDO010000000
VERSION     DAOQDO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18681_length_3638_cov_2.901758"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..403
                     /locus_tag="PKZ53_06830"
     CDS             32..403
                     /locus_tag="PKZ53_06830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ40184.1"
                     /translation="MKELKIEDVAVGKGAAAKTGQEVTVHYTGTLLNGTKFDSSRDGG
                     KPYTFTLGAGTVIKGWDQGIVGMKAGGKRKLTIPADLAYGSSGRPPVIPPDSPLLFDV
                     ELVSVKDAAKPAAADKDAKKK"
     gene            507..1298
                     /locus_tag="PKZ53_06835"
     CDS             507..1298
                     /locus_tag="PKZ53_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40185.1"
                     /translation="MEIRIEITDRDIQDLVCQNLQTVLLRLAGSLYRAPLSVSGQIPQ
                     FRIIEQSLDLGQPYGCLGILAADHKNSSLLINVAKGEIGIPDIVRLLQVAALLRSQLM
                     TSLQVRLGSIQDQNRSQGATASSLFTSFEDEMKDRGTTALIYPVVIGARFTNEALLLM
                     DGLNSQVAKAPMISNSTPGMIFAGTYHLSIELTGTSVETPQVQFAVEDALSTATYSVG
                     SSQTPGREILKTYADETGDRLIREFLDYEQQKRAHKSGMFRAYQE"
     gene            1561..2319
                     /locus_tag="PKZ53_06840"
     CDS             1561..2319
                     /locus_tag="PKZ53_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ40186.1"
                     /translation="MPTPIDFWQKQNHGLRVAFIDGCKHEATHMAMHQVPLDLELTLD
                     AAEQRLNDLVEYQVIIVGVTVYPIRRRVIGELRRVNSQAALIFLRLSPGVGAGESGLE
                     VRDQVIADIMLSSNSPQIVWQAAESLCPIFPFPASPDMKRPPEASLVEKAVRVISMYY
                     TDPSLNLHRVADKLGLSAGQLSRILNKHAGMRFRQLLQQTRLEAAKQMLAAGNTSIKE
                     VAYSVGFSDSDYFSRAFKRYTGDCATTFKGSTFS"
     gene            complement(2370..>3638)
                     /locus_tag="PKZ53_06845"
     CDS             complement(2370..>3638)
                     /locus_tag="PKZ53_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40187.1"
                     /translation="LDQVFQATVDELRDILDASRCVIFEIDEPGEFLVTRCESVKEAA
                     LSVQAYQIPLRNAPIGRNVMMKGETVTIAEMAPLVEPPLLNREHDKYLSVVNQQVLQA
                     LMPKALFATPLRARGQVVAGLAIHQCDRPRRWAPDEVEFIIAIANQTGLAIERAQLFQ
                     EIQERNHQLEETVGKLEELARQREEFTAVLVHDIRSPLSVVLGVLELLEFRAQDLGIL
                     DENLTQLCVDGFTTVNNIVSLVNEILDFSKAEAGGLQLELSLSNPVDVVQSAVHQVDL
                     LATQKQITITLNHESNLPMLLLDAPKINRALVNLLSNAVKFTPVAGEITITTRLIEGK
                     ELEEGKQFVGISVIDNGPGIPAQDLPYLFNPYYQARQRTRHLGTGLGLAIVQRILAAH
                     GGKVLVQSTEGLGTAFSIILPVLVSQEVTP"
BASE COUNT          889 a          914 c          938 g          897 t
ORIGIN      
        1 agagcctgaa ccgaccacac cgaccccagc cgtgaaagaa ctcaagattg aagatgtcgc
       61 ggttggaaaa ggtgctgctg ccaagactgg tcaggaagtg acggttcatt acactggaac
      121 cctgctgaat gggacgaaat ttgacagttc acgagatgga ggtaaaccgt atacctttac
      181 acttggggct ggaactgtga tcaaaggttg ggatcaaggc atcgttggga tgaaagcggg
      241 tggaaagcgg aaactgacaa tcccggctga tttggcatat ggatctagcg gtcgtccgcc
      301 ggttattcct cctgattccc cgttactgtt tgatgtcgag ctggtttcag tcaaagatgc
      361 cgctaaacca gccgccgccg acaaagatgc caaaaagaag taaagagagc ctgaaaagag
      421 cctgaggccg ccagttttca gactggcggt catccggcgg tggcactcta tctttgggaa
      481 ttgaacttgt tgagagtgag gaagttatgg agattcgaat cgaaattacc gaccgcgata
      541 tccaggatct ggtgtgtcaa aatctccaga ctgttttatt gcgattggct ggaagtctct
      601 atcgggcacc cctgtcagta tcagggcaaa ttcctcaatt tcgaatcatt gaacagtcac
      661 ttgaccttgg ccagccgtac gggtgtttag ggattctggc tgccgatcat aaaaatagct
      721 cgttgctgat caatgttgcc aaaggtgaaa ttggaatccc agacattgtg cgcctgctcc
      781 aggtggccgc actgttgcga tcccaactga tgacttcgct ccaggtgcga ttagggagta
      841 ttcaggacca gaaccgctct cagggggcaa ccgccagttc actctttact tcgtttgaag
      901 atgaaatgaa agatcgggga acgacagcgc tgatctatcc ggttgtcatt ggcgcccggt
      961 ttaccaatga agcgctcttg ctgatggatg gattaaattc ccaggttgca aaagccccga
     1021 tgatctctaa ctcaacccca gggatgatct ttgccggaac ctatcacctc tcgattgaac
     1081 tcaccgggac cagcgtcgag acgccacagg tccagtttgc ggtcgaagat gccctcagca
     1141 ctgcaaccta tagtgttgga tcctcacaga caccaggacg ggaaattctc aagacctatg
     1201 cggatgaaac cggggatcgg ctgattcgtg aatttttaga ctatgagcag caaaaacggg
     1261 cgcataaaag cgggatgttc cgggcgtatc aggaatgaat tgcccgctgg gtgcagggtc
     1321 gtttttccct gaaattctga gggtgaaacc ggtacaggat aaacgacgca ccagttcttc
     1381 acctaaattt ctgggttgcg gcataaatcc atttgctttc ttgtcttttc cagtttaggc
     1441 ttacgcaagt catcacttca tcattccaat cgttgatttc atttcccaat cctaaaagtt
     1501 aacaatcacc tgacgcttta gatttgacac tcgaaacttt ttaagggttt ggaggaaact
     1561 gtgcccacac ctattgattt ctggcaaaaa caaaatcatg gactgcgcgt tgcgttcatt
     1621 gacggatgta aacacgaagc cacccacatg gcgatgcatc aggttccact cgatttggaa
     1681 ctgacgctgg acgcggctga gcaacggtta aatgacctgg tggagtatca ggtgatcatt
     1741 gtcggggtga cggtttaccc aatccgccga cgggtcatcg gtgaactgcg gcgagttaac
     1801 tcacaggccg cactcatttt cttacgactc tcacctgggg tcggcgctgg tgaatctggt
     1861 cttgaggttc gtgatcaggt gatcgctgac atcatgctca gttccaactc cccgcaaatc
     1921 gtctggcaag cggcagaatc cctgtgtccg atctttccgt ttccggccag cccggatatg
     1981 aagcggccac cggaagcctc gctggttgaa aaggccgtgc gggtgatttc gatgtattac
     2041 actgacccct cgctcaattt acatcgagtc gctgataaac ttgggctctc agccggtcag
     2101 ttatcgcgca ttttgaataa acacgctggc atgcgcttcc gtcagctttt acaacaaacc
     2161 cgtcttgagg cagccaagca aatgttggcg gctggaaaca ccagcattaa agaagtcgct
     2221 tacagtgttg gctttagcga tagtgactat ttttccagag cttttaaacg gtacaccggt
     2281 gattgcgcca ctacgtttaa gggaagcacg tttagttaat ttgacggacg gtatgccaga
     2341 gggtacggaa caggtgggcc tcacgctgat caaggggtta cttcctgaga gaccagaact
     2401 ggcaatatga ttgagaaggc cgttcccagt ccctctgtgc tctgaaccag aacttttcct
     2461 ccgtgagccg ccaggattcg ttgcacaatc gccagtccta acccggtacc caggtgccgg
     2521 gtacgttgcc gggcctggta atagggatta aacagatagg gcaggtcctg ggctggaatg
     2581 cctgggccat tatcaatcac gctaattccc acaaattgct ttccttcttc taattccttg
     2641 ccttcaatca gtcgggttgt aatggtgatc tcacccgcga cgggcgtgaa tttgaccgcg
     2701 ttgctcagta aattcaccag cgcacggttg attttaggcg cgtcgagcaa cagcatcggc
     2761 agatttgatt cgtgattgag ggttatggtg atttgctttt gggtggccag cagatctacc
     2821 tgatggacag ctgattgaac cacatccacc ggattactca gactcaactc aagttgtaac
     2881 cccccagcct cagcttttga aaaatcaagg atttcattga ccagtgagac aatattattc
     2941 accgtggtaa agccgtctac acagagctga gtcaggtttt catccaaaat ccccagatcc
     3001 tgagcccgaa actcaagcaa ttccagcacc cccagcacca cagaaagcgg tgatcgaatg
     3061 tcgtggacca gaaccgctgt gaattcttcg cgttggcggg ccagttcttc cagtttgcca
     3121 actgtttctt caagctgatg gttgcgttcc tggatttcct gaaagagctg ggctcgttca
     3181 atggcgagtc cggtttggtt ggcaatggcg ataataaatt ccacttcatc aggcgcccag
     3241 cgacgagggc gatcacactg gtggattgcc agcccggcaa ctacctgccc acgggctcgg
     3301 agtggggtcg caaacagggc tttgggcata agtgcctgta aaacctgctg attgacgacg
     3361 ctcaggtatt tgtcgtgctc ccgatttaaa agaggtggtt caaccagggg ggccatctca
     3421 gcaatggtga cggtctcacc cttcatcatg acattgcggc caatcggagc atttcggagg
     3481 ggaatctgat aggcctgaac gctcaaagca gcttccttca ccgattcaca ccgtgtcacc
     3541 aaaaattccc caggttcatc aatttcaaag atgacacacc ggctcgcgtc taaaatatcg
     3601 cgcaattcat caaccgttgc ctgaaagacc tgatccag
//
LOCUS       DAOQDO010000341         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18747_length_3630_cov_2.677203, whole genome shotgun sequence.
ACCESSION   DAOQDO010000341 DAOQDO010000000
VERSION     DAOQDO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18747_length_3630_cov_2.677203"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PKZ53_06850"
     CDS             <1..1552
                     /locus_tag="PKZ53_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40188.1"
                     /translation="IRDLPGSTPAREQVVKKALVYLNRLQAEAGSDPELQLELATAYQ
                     RIGDIQGHPITNNLGQPAEALVSYQRALVIREALGTQPQGQTPQFQSDLAENLARIGD
                     MMVYTGQAANGLEYYRRAIGLCELLVAARTSLRARLKLPYLYCQYGFTLVYNGNQEAA
                     LNAYRKSVELAEVLHQEDPNNLEIEYDLAQNLGGYGDQVYALGNPQKGLELLKESRRH
                     LEHLTRLEPNHTNWFSGLALANVRIGNLLMDTGDPYQSIPYHQQAKEVRTSLAAKDPT
                     NMRAQAARITTLFGYARTFVLIGDTQRAITEYTQVLQIAEFLAQSNPGYVLHQRTISE
                     LLLILGTLEIKQNNLEKATKMLRRAKEVYERLYQQDPNHSQFRAELPVVLRTLASTLS
                     HQGQSEEAQALTKRALEMQVTLADRPDALVNEIYEYAVTLVTGEPESLRDPVRGLEFA
                     LRAVEKTNRKAVDCLTLLARAYLLSGNHKAALATIEEALRLIPGDKPTAFRKELETLR
                     RQSMGSRS"
     gene            complement(1643..2869)
                     /locus_tag="PKZ53_06855"
     CDS             complement(1643..2869)
                     /locus_tag="PKZ53_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40189.1"
                     /translation="MQRTQKFLLIELLLCLYAVGSSILVLSFPLSKPGRNSAPKPSAS
                     AQIAVEVDSPNWQFTDPKAHVEEHLGRKSLFIQSGFALLKGVDFENGTLEFDFAAPKV
                     PAFIGAAFRAENSDDHEIVYFRPHKSKLPDAVQYTPSFNGGAPWQIYSGKGFTSAVEI
                     PRGEWVHVRIEIAGLGGKVFLNNSAEPVLVIEDLKRGYSHGTVGLWAIAGGGHFSNFS
                     YRVDPVTDRPEKKPPMYDAGILPQWELSEAFEASTKNPEHLLSPAEMKALKWQAVGVE
                     VPGMVVIDRYRHSPGVVPSFGTLAERTGKGEKRRFVFARTTIYSDRDQVKRLSIGYTD
                     EATLFLNGSPVFTGRSAYRFRDPGFLGIMDVENDTLYLNLKKGRNELVLTVAEYFGGW
                     GFICRLDDMQGVKLEN"
     gene            complement(2940..>3630)
                     /locus_tag="PKZ53_06860"
     CDS             complement(2940..>3630)
                     /locus_tag="PKZ53_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ40190.1"
                     /translation="TLLTPPASLFIIHPGEVHANRALAATGCSYRTLFLTIHQMHQVA
                     QELQGTATPLPFFPSPIVVESHLLDCFERTHLALEATTSSLERQTRLLNLITQLIVQV
                     SDSRPELHPTGSDRKILRHACDYLVEHFAENILLEELASLSNLSPFHFNRMFSLHFGM
                     PPHAFQTQLRVMRAQALLRQGWSIPQVASQTGFADQSHLNRHFKRVTGLTPGQYRQNR
                     KNVQDSLTHIL"
BASE COUNT          940 a          893 c          941 g          856 t
ORIGIN      
        1 gatccgggat ttaccgggct cgactccggc gcgtgaacag gttgtcaaaa aagcacttgt
       61 ctacctgaat cgcctacaag ccgaagcagg ctcagaccct gaattacaac tggaactggc
      121 caccgcctat cagcgcattg gggatattca ggggcatcca attaccaaca atctcggtca
      181 gccggctgag gcgttggtca gttatcaacg ggctctggtc attcgtgaag cacttggcac
      241 ccagcctcaa ggccagactc ctcaatttca atccgatttg gctgagaatc tggctcgcat
      301 tggggacatg atggtatata ccgggcaggc ggccaatgga cttgagtatt atcgaagggc
      361 aattggtttg tgtgagttgc tggttgcagc tcgaacttca cttcgggccc ggctcaagtt
      421 gccatatttg tactgccagt atgggtttac ccttgtttat aatggcaatc aggaagcagc
      481 tctgaatgcg tatcgaaaaa gtgtcgaact ggctgaagtc cttcaccagg aagacccaaa
      541 taatctggag attgaatatg atctggccca gaatctgggt ggctatggtg accaggttta
      601 tgcactggga aatccgcaaa aaggattaga gttactcaaa gaatcccggc gccaccttga
      661 gcatttgacc cggctcgaac ctaaccacac aaactggttc agtggtttag ctctggctaa
      721 tgttcgcatt ggaaatctct taatggacac tggcgatccg tatcaatcca tcccatatca
      781 ccagcaagca aaagaagtca gaaccagtct ggctgccaag gaccccacaa atatgagagc
      841 ccaggccgcc agaatcacaa ccctctttgg atatgccaga acttttgttc tgattggtga
      901 tactcaacga gccatcaccg aatatacaca ggttttgcaa attgctgaat tccttgccca
      961 gagcaatcct ggctatgtac tccatcaacg taccataagt gagttattac ttattttggg
     1021 cacattagaa atcaaacaaa acaatctgga aaaagccaca aaaatgttgc gtcgggcaaa
     1081 agaggtgtat gagcggttgt atcaacaaga tccgaaccat tctcaatttc gagctgagtt
     1141 gccggtggtg cttcgaactc tcgccagtac gctgagtcat caggggcagt cagaagaagc
     1201 tcaggcgtta accaaacgcg ctctggaaat gcaggtcacc cttgcagacc gtccagatgc
     1261 cctggtcaac gaaatttatg aatatgccgt caccctggtt acgggtgagc cggaatcatt
     1321 gcgcgatcct gtccgtggat tggagtttgc gcttcgggcg gttgagaaga ccaatcgaaa
     1381 agccgttgat tgcctcacgc ttttagcccg cgcctatctg ctttcaggga atcacaaagc
     1441 cgccttagcc acaattgagg aagcgttgcg tttgattccg ggtgacaaac caacagcttt
     1501 tcgcaaagaa ctggaaacac tccggcgaca atctatgggc agtaggagtt aggggtccgg
     1561 ggtttaaagg acaaaaagga cgaaaaagac ataaagggca taaattcaaa gaccctgaac
     1621 cctgaaccct gctggatccg cttcaattct ccaacttgac gccttgcata tcatccaggc
     1681 ggcaaataaa accccagccg ccgaagtact ccgctacggt caggaccagt tcgttgcggc
     1741 cttttttcag attgagatag agcgtgtcgt tttcaacatc cataattccc aaaaatcctg
     1801 ggtcgcggaa gcgataggca ctccgacccg taaagacagg tgaaccattg aggaagagtg
     1861 ttgcttcgtc ggtgtagcca atagacaggc gttttacctg gtcacggtcg gaataaatcg
     1921 tggttcgggc aaaaacaaac cggcgttttt cgccctttcc agtgcgttcg gcgagtgttc
     1981 caaacgaagg cacgacgcct ggactgtggc gataacggtc aatgaccacc atgcctggca
     2041 cttcaacccc aaccgcctgc catttcaggg ccttcatttc ggctggagac agaaggtgtt
     2101 ctggattctt ggtactggct tcaaaggctt cagagagttc ccattggggc aggatccctg
     2161 catcatacat tggcggtttt ttctccggac gatcagtcac gggatcaact cgatatgaaa
     2221 aatttgagaa atgcccgcca ccggcaatcg cccagagccc aactgtgccg tggctgtatc
     2281 cgcgtttcag gtcttcgatc accaggactg gctcggcgga attgttcaaa aagactttgc
     2341 cgccgagtcc ggcaatctcg atccggacat gcacccactc gcccctcggg atttcaactg
     2401 cactggtaaa tcctttgccg ctatagattt gccacggtgc accaccatta aatgacggcg
     2461 tatactggac cgcatccggc agcttgcttt tgtgtggacg aaagtaaacg atttcgtggt
     2521 catctgaatt ttcagcccgg aatgcggctc cgataaaggc tgggactttc ggcgcggcga
     2581 aatcaaattc aagcgtgccg ttttcaaaat caacgccttt taacaaggca aaccccgatt
     2641 ggataaacag gcttttgcga ccaagatgct cttcaacatg tgctttaggg tcagtgaatt
     2701 gccagtttgg cgagtctact tcaacggcaa tttgagccga tgccgaaggt tttggggccg
     2761 aatttcgccc aggcttcgag agtggaaacg aaagcaccag gatgctgctt ccaacggcgt
     2821 acaggcacaa cagaagttca atcaacagga atttttgagt acgttgcatg actagagaca
     2881 cctctcacag caggttgccg gccttctgag tttgaaattg ctgaattgga attcaaaact
     2941 cagaggatat gcgtgaggct gtcttgaacg ttcttgcggt tttggcggta ttgccctggg
     3001 gtaaggccag ttactcgttt gaaatggcgg ttgagatggc tttgatcggc aaacccggtc
     3061 tggctggcaa cctgtggaat tgaccatccc tggcgcaaca gtgcctgagc acgcatgacc
     3121 cgaagttggg tttgaaacgc gtgtggcggc attccaaagt gaagggagaa catacgattg
     3181 aaatgaaacg gactcaagtt tgaaagactg gcaagctctt ctaataaaat gttttctgca
     3241 aaatgctcaa ccagataatc acacgcgtgc ctgaggattt tacgatctga gccagtcgga
     3301 tgaagttcag gacgcgaatc cgatacttga acaataagct gggtaatgag attcagaagc
     3361 cgggtttggc gttccagact ggatgttgtt gcttccaaag ccagatgggt ccgctcaaaa
     3421 caatccagga ggtgggactc aaccacaatc ggcgatggaa agaagggaag cggagtggcg
     3481 gttccctgga gttcctgtgc cacctgatgc atctggtgga ttgtcaaaaa tagcgtccgg
     3541 taactacaac ccgttgccgc cagtgcccga ttggcgtgga cttcaccagg atgaatgatg
     3601 aacaaactgg ccggaggcgt caggagtgtt
//
LOCUS       DAOQDO010000342         3619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18828_length_3619_cov_2.475589, whole genome shotgun sequence.
ACCESSION   DAOQDO010000342 DAOQDO010000000
VERSION     DAOQDO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18828_length_3619_cov_2.475589"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>3619)
                     /locus_tag="PKZ53_06865"
     CDS             complement(49..>3619)
                     /locus_tag="PKZ53_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40191.1"
                     /translation="RKDGTPFWNQLVVTPIFNHRGAPAYFVGVQTDVTDRRRVELQLQ
                     QKEQLFEYGPAVIFRWETTWPYRTISVSANVKQFGYDPIDLVNGVIEWIDIIHPDDRE
                     RVHLEVQTLQQQHPLPTFAEQYYRIVCADGSVRWVYDYTVPVLDKNGGLVFADGYLLD
                     ITRRKQVEDALRESEGRLRRIGDNLPNGALYQIVISPEGNRKYTYVSAGAERMLGYSI
                     EALMADADLAYNSVLEADVPELLAVESKAWAELSVFQHTVRVVTTTGELRWIHCQSAP
                     QRNPDGSTVWDGVVIDITQSRYAEESLKRSERFLNAVFENSPVAMEVFSPDGYLLRIN
                     PAMSRFLGYSDGGAFAIGKYNILTDPVAQSLNSPTWFKRALQGETVEARSQTVDLKKV
                     QEWSVTQRVVYYDVAFVPIADDSGMVTNVVVMIWDITAQKEAERALRQKEQLLQQAAK
                     IAKIGGWELNIPAQKLSWSEETYRIKEVESKFEPSIESAINFYAPEVRPVIRAAVEKG
                     IYDGEGFDLELPLITGTGRLLWVRALGEPEFENGVCVRLAGALQDITERKITELALKA
                     SEERFREILNALPQLVGLVDRELRYQFVNKTYLTFFQVEETQIQGKTLPEVIGAKAFS
                     QLKPLIERVLSNEVVHFCQSVHFRHVTAWLDGYLIPHRSTSGEVIGYFAVITDVTQFK
                     EAETALRENAETYQTLLNTTMDGFCIVDSHEQYVDANDVYCQMLGYTRQELLTKKIRE
                     IDTKWTDSEITAQLKKIVERGGDQFETINRTKDGRLVDVEIRCTYLANKCQILVFARD
                     ITAQKQAEEQLRQAQKMEAIGRMAGGIAHDFNNVLTIIIGYINILQQRLGPGHNCYPI
                     SEQLLKASERGAALAQQLLTFSRKQKVKLRPINLNDSVIAIEQFLRRLVGDELELILL
                     MDEGLGMIRADAGQLEQVIMNLAVNARDAMPNGGKLIIETSNIELDEEFTQFHPELHP
                     GKYILLGVTDTGVGMDEQIKACIFEPFFTTKDTGRGTGLGLAIVYGIVQTCGGHIVVD
                     SMLGVGTSFSIYFPWIQTGLLGERSNSPDQAVRKPKKLILLVEDEAEIRGLLTRLLEE
                     QGYAVLTAENGFEALQVATEYGEPIQLLVTDIRMPKMDGLELVRVLSPHHPEMKILYL
                     SGYVDAGLRKNEAFMQSCFLEKPFPPDSFLDKVQELLGDRETE"
BASE COUNT          893 a          970 c          825 g          931 t
ORIGIN      
        1 ccaagcacca agcaccaagc accagataca ttcttcattc ttcattcttc attctgtctc
       61 tctgtcaccg agtaactcct gcactttgtc aaggaatgag tcgggaggaa aaggtttttc
      121 caggaagcac gattgcataa acgcctcgtt tttgcgcagc ccagcatcca catatcctga
      181 cagatacaga atcttcatct ccgggtgatg tggagaaagc actctcacca gttcgagtcc
      241 atccattttg ggcattctga tatcagtcac cagcaattga atcggctctc catattcagt
      301 ggcaacctgc aacgcctcaa acccattctc ggctgtcaga acagcatagc cttgctcttc
      361 tagtagtctt gtgagcagtc cgcgaatctc agcttcatcc tcaaccagca agatcagttt
      421 tttcggttta cggacagcct ggtcgggaga attactgcgc tcgcccagta gcccagtctg
      481 gatccacgga aaataaatcg agaagctggt cccaacaccc agcatgctat caaccacaat
      541 atggccgccg cacgtctgga cgatcccgta gacaatcgcc aaccccaggc cagttccacg
      601 accagtatcc ttggttgtga aaaacggttc aaaaatacag gctttgatct gctcgtccat
      661 gccgacaccg gtatcggtga ccccaagcaa aatatatttg ccggggtgga gttcaggatg
      721 aaattgggta aattcctcat ccagctcgat atttgacgtt tcaatgatga gctttccacc
      781 attgggcatc gcatcccggg cattgaccgc caggttcata atgacctgtt cgagctggcc
      841 agcatcagca cgaatcatcc ctaacccctc atccatcaat aagatcaatt ccagttcgtc
      901 gcccaccagt cggcgcagga actgctctat cgcgatgacc gaatcattga ggttgatcgg
      961 ccttaacttt actttctgtt tgcggctaaa cgtcaataac tgctgggcca gcgccgcgcc
     1021 gcgttcactg gcttttaaaa gttgctcact aatggggtaa cagttatggc caggccccag
     1081 tctctgctgc aagatgttga tatagccgat gataatggtc agcacattgt tgaaatcgtg
     1141 ggcgatgcct ccggccatac gaccaatggc ttccattttt tgggcctggc ggagttgttc
     1201 ttcagcttgt ttttgggcgg taatatcacg agcaaaaact agaatttgac atttattggc
     1261 caggtaggta caccggattt caacatcaac cagccgcccg tctttggtgc gattgatggt
     1321 ctcaaattga tcgccacccc gctccacaat ttttttgagc tgcgcggtta tctctgaatc
     1381 agtccatttg gtatcaattt cacggatttt tttggtcaag agttcctgac gagtgtagcc
     1441 cagcatctgg cagtagacgt cattggcatc aacatactgc tcatggctat caacaataca
     1501 aaacccgtcc atggttgtat tgagtaaggt ctgataggtt tccgcattct cccgaagtgc
     1561 cgtttcagct tctttaaact gagtaacgtc ggtgataacg gcaaagtatc ctataacctc
     1621 tccgctggtg gaacgatggg gaatcaaata tccatccagc caggccgtga catggcgaaa
     1681 gtgaacggat tgacaaaaat ggaccacctc gttgctgagg acccgttcaa tcaatggttt
     1741 caattgggaa aaagccttcg ccccaattac ttccggaagc gttttccctt gaatttgggt
     1801 ttcttcaacc tgaaaaaatg tcagataggt tttattgaca aattgatagc gcaactcacg
     1861 gtcaacgagc ccaaccagtt gcggcaacgc attgaggatc tcgcggaatc gttcttcact
     1921 tgccttgagc gccagttcag ttattttgcg ttcagtgatg tcctgcaggg ccccagccag
     1981 acggacacac accccatttt caaattcggg ttctcccaag gcacgaaccc acaataatct
     2041 accagtccct gtgatcaagg gtaattccag gtcaaagcct tccccatcat agattccttt
     2101 ttcaaccgcc gcccggatca cgggtctcac ctcaggggca taaaaattaa tggcggattc
     2161 aatacttggt tcaaactttg actcaacctc tttgatacgg taggtctctt ccgaccagga
     2221 caatttttga gctggtatat tgagttccca gccaccgatt ttggcgattt tagccgcctg
     2281 ttggagcaat tgttcctttt gacggagtgc tcgctcagct tctttttggg cggtgatatc
     2341 ccaaatcatg acaacgacat tggtgaccat ccctgaatca tcagcaattg gcacaaatgc
     2401 cacatcataa tagacgactc gttgagtgac tgaccattct tgaaccttct tcaaatcaac
     2461 ggtttgggag cgggcttcta ctgtttcccc ctgcaacgct cgtttgaacc aggtgggtga
     2521 gttcagcgat tgggcaaccg gatcggtcaa aatattatat ttcccgatag caaaggcccc
     2581 gccatcggaa taacctaaaa agcgactcat cgccggattg atccgaagca aatatccatc
     2641 cggtgaaaac acttccatcg cgacgggcga attttcaaac accgcattta agaaccgctc
     2701 gcttcgcttg agtgattctt cggcgtatct ggactgggta atgtcaatca cgacaccatc
     2761 ccacaccgtt gacccatccg gattccgttg cggggcagat tgacagtgga tccatctcaa
     2821 ttctccggtt gtggtgacga cacggaccgt gtgctgaaag accgagagtt cagcccaggc
     2881 tttggactca acagccagga gttcgggcac atccgcttca agaactgagt tgtacgccag
     2941 atcagcatcc gccataaggg cttcgattga gtagccaagc attcgttcag ccccggcgct
     3001 gacataggta tactttcggt ttccttccgg agaaatcaca atctggtaca gggcgccgtt
     3061 gggcagatta tctccgatgc gccggagccg gccctcactt tcgcgtaagg catcttccac
     3121 ctgttttcgc cgggtaatgt ccaacagata cccatcagca aagaccagtc ctccattttt
     3181 atccaacacc ggtacggtat agtcatacac ccatcggact gagccatcag cacaaacgat
     3241 gcgatagtat tgttcggcaa acgtcggaag cggatgttgc tgctgcagcg tttgaacttc
     3301 caaatgaacc cgttcacggt cgtcagggtg gataatgtca atccattcga tgactccatt
     3361 caccaaatca atcggatcat acccaaactg cttcacattg gccgaaactg agatagttcg
     3421 atagggccag gttgtttccc atcggaaaat cacggcgggt ccgtactcaa agagttgttc
     3481 tttctgctgg agttgcaact caacccgtct ccggtcggtg acatcggttt gcacaccgac
     3541 aaaataggct ggtgccccac gatgattaaa gattggggta acgactaatt ggttccaaaa
     3601 cggggttccg tctttgcgg
//
LOCUS       DAOQDO010000343         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18869_length_3615_cov_2.088483, whole genome shotgun sequence.
ACCESSION   DAOQDO010000343 DAOQDO010000000
VERSION     DAOQDO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18869_length_3615_cov_2.088483"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="PKZ53_06870"
     CDS             complement(<1..2182)
                     /locus_tag="PKZ53_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40192.1"
                     /translation="MKTFYRILVGIFVLSFLFTYGYHIFQSTELTTLASTTQKSLHRQ
                     TPTISSISPGQVPKGQSPAKIKIVGTGFIAGTRVFFSEQEVPAKVKASGRKIILKDLA
                     ASLFAQDRTYDVVVQTPAGQRSNIVKLVVGTGVNPAEPASIQVSQPQSLALNTSTSTQ
                     ITARVLDKNGVEIPGAQVTYQSDNTAVASVSATGLVSAITEGAALVQLQSGAVTTQLM
                     FRVSRVDSVPSGIFGQSDMANFAGTIYASDLSKHIIRRSQSFQPMNDYAGQSGVAGNL
                     DGTLTGSRFNAPLGIGFRNGMLYLADTNNQTVRQLNTATGAVRTIATLSNATAASGVS
                     SWGPRDVEIDVNNDLYITDAVNHVVWQATLQNSSVQMSVLAGRIGQPGTVDGQGTGAM
                     FNTPQRITLTGKILSVTQTGNQVRQIALPGGAVKTIQSQAGRPSDVSDLARINQSEDF
                     EPIEFEDESQSWSEDGALDFNAQNPLQLTQPSAIASDADGNLYVATTGSVQILTITND
                     RAILSDLVAPGTLQNPVALTVSPDAVFALDGNTGQLVRIQFGKPSISQISPSQVAAGS
                     SSNQITVTGDNFYFDTQVFLGSQAVSNVTVLNSKQLQFTLPPQPFGGSVTVRVRNRGG
                     EARGLLTVTGTPAPATVKLNAFPSSRTVAQGESAFFTIELDRTNFEENVNLAVTGLPS
                     GATAQFSANPTTASAVQLKVDVTTRVTSGNYTLQITGTGSGATVT"
     gene            complement(2264..3367)
                     /locus_tag="PKZ53_06875"
     CDS             complement(2264..3367)
                     /locus_tag="PKZ53_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNJ40193.1"
                     /translation="MPYVNLKPIIDHMLKQSEHVSDLNFSVNCPPQVEINGELFPVNI
                     KGLPVLTAYHTEIIAMTLLGGNKDAAEKLIRNGSVDLSFSVSGWNRFRVNVFLQRGTI
                     SVVMRVVLSDIPSLESLGLPTQLGSISTLKNGIVLITGPSGSGKSSTLAALVDKINQE
                     RSYHIVTIEDPIEFLHTRKRSTINQRELGSDTPNFAVALRAALRQSPKVILVGEMRDL
                     ETTEIALEAAETGHLVLSTLHTTDASKTIDRLIGLFPKAEERVIRVRLAQAFQFIVSQ
                     RLIPKAKGGGRIAAVEILKSTPRTREYIEKGESQGKTLLDAMQDGNLEGMQPFDQVIE
                     RYIRTGMVTREDGLSFATNPHNLELRLAGLGAG"
BASE COUNT          894 a          917 c          886 g          918 t
ORIGIN      
        1 gggtcacagt ggcgccagat ccggttccag taatctgtag agtatagttt ccagatgtca
       61 cgcgtgtagt gacatccact ttcagttgta cagcactggc ggtggttgga ttggcactaa
      121 actgagcagt ggcaccactg ggcaacccgg tcaccgccag attgacgttt tcctcaaaat
      181 tggtccggtc aagttcaatc gtaaagaagg ctgattcgcc ctgcgcgacg gtccggctcg
      241 aaggaaaagc attgagcttc accgttgctg gtgcaggagt tccggtcaca gtaagcaacc
      301 cacgcgcttc accgccccgg ttgcgaaccc gaacggtcac actcccgcca aacggttgag
      361 gaggaagggt aaattgcaat tgtttggaat ttaaaaccgt cacgtttgaa acagcctggc
      421 tgcccagaaa aacctgggtg tcaaagtaaa aattgtcacc agtgacggta atttgattac
      481 tgctactccc ggctgccacc tgtgatggac tgatttggga gatactcggc tttccaaatt
      541 gtatccgaac aagctgtcca gtgttgccgt ccagagcaaa tacagcgtcg ggactcacgg
      601 tcagggcaac gggattctgg agcgttcctg gagcaaccag atcagagaga atcgcccgat
      661 cattggtaat ggtcaggatc tgaacgctcc cagttgttgc cacatacaaa tttccatctg
      721 catctgaggc aatggctgag ggttgggtca actgaagcgg attttgagca ttgaaatcca
      781 gcgcgccatc ctcactccag ctctgtgact cgtcttcaaa ctcaatcggc tcaaagtctt
      841 ctgattgatt gattcgggcc aggtcgctca catccgatgg tcttccagcc tgactttgaa
      901 tggttttgac cgcaccgcct ggcaaagcaa tttgccggac ctgatttcca gtttgggtca
      961 cactcaggat ttttccagtt aaggtaatcc gttgcggagt gttaaacatc gccccggtac
     1021 cttgcccatc cacagtccct ggttgcccaa ttcggcccgc cagcacactc atctgaactg
     1081 atgaattttg aagtgttgcc tgccagacca catggttgac cgcatcggtg atataaagat
     1141 cattgttgac atcaatttct acatcgcgcg gtccccagct tgaaacacca ctggctgccg
     1201 tggcattgct cagcgtagca atggtccgaa ccgcaccagt tgcggtatta agctgacgaa
     1261 cagtttgatt attggtgtca gccagataga gcatcccgtt tcggaagccg atgccaagcg
     1321 gtgcattaaa ccgtgagccg gttaaggttc catcaagatt tccggcgaca ccggattgtc
     1381 cagcataatc attcatcggc tgaaatgact ggcttcggcg gataatatgt ttcgacagat
     1441 cagatgcgta aatcgtcccg gcaaaattgg ccatatcact ttggccaaag atacctgatg
     1501 gaaccgaatc cacccgactg acccgaaaca ttaactgcgt ggtaacggca ccggattgaa
     1561 gctggaccag ggccgccccc tcagtgatgg cactgaccag accggttgca gaaaccgatg
     1621 ccacagccgt gttgtcagat tggtaggtaa cctgggcgcc ggggatctca actccattct
     1681 tatccaggac ccgagccgta atctgggttg aggtactggt attgagtgcc agcgattggg
     1741 gctggctcac ctgaatagag gctggctcag ccggattgac gcccgttcca accaccagtt
     1801 tcacaatatt ggaccgctgc cctgctggtg tttgaaccac cacatcatag gttcggtcct
     1861 gggcaaagag tgaggcggcc aggtctttga ggataatttt ccggcctgac gctttgactt
     1921 tggctggcac ctcctgctcg ctgaaaaaca ctcttgttcc agcaataaat ccagtgccaa
     1981 caattttaat tttcgcagga ctctgtcctt tcggcacctg accaggtgaa attgaactaa
     2041 tcgttggggt ttgtcggtga aggcttttct gagtcgttga agccagtgtg gttaattcag
     2101 ttgattgaaa aatatggtat ccatatgtaa ataaaaagct taaaacaaaa ataccaacca
     2161 ggattcgata gaaggttttc ataaaaagtc tccaaataaa cggcaattga gcaaaacgtg
     2221 acgggcacaa tacgttttgt ccctgcccaa cagatgaagt gcttcatccg gcacccaatc
     2281 cagccagcct caattcgagg ttatgcggat tggtcgcaaa tgacagcccg tcttcacgcg
     2341 tgaccattcc ggttcgaatg tatcgttcaa taacctgatc aaacggctgc atgccttcca
     2401 gattcccgtc ctgcatcgca tcgagcaggg tttttccctg actttcgccc ttttcgatgt
     2461 attcacgggt tcgaggcgtt gattttaaaa tttcgacagc ggcaatccga ccgccgcctt
     2521 ttgcttttgg aatcaatcgt tgtgagacaa taaattgaaa tgcctgggca agtcggacgc
     2581 gaataacccg ctcctcagct tttgggaaaa gcccaatcag gcggtcaatt gtttttgaag
     2641 catcggtcgt gtggagggtg gaaagtacca gatgcccggt ttcagccgct tcaagtgcaa
     2701 tttcagtggt ttctaaatcc cgcatttctc caaccagaat cacttttggc gactggcgca
     2761 aagccgctcg tagcgcaacc gcaaaatttg gcgtatcgct gcccagttca cgctggttaa
     2821 tcgttgatcg ctttcgggta tgcagaaact caatcggatc ttcaattgtg acaatatgat
     2881 aggaccgctc ctggttgatt ttgtctacca gggcagccag ggttgaggat ttcccactcc
     2941 cactgggacc tgtaatcagc acaattccat ttttgagggt tgagatactt cccagttggg
     3001 tcgggagccc cagtgattca agcgatggaa tatcagacag gaccacccgc atcaccactg
     3061 aaatggtgcc acgctgcaaa aaaacattga ctcgaaaccg attccaccca gaaacactga
     3121 acgataaatc cactgagcca tttcgaatta acttctcggc ggcatcttta tttcctccca
     3181 acaaggtcat ggcaatgatt tcagtgtggt aggccgttaa tacgggcaac cctttgatat
     3241 tgaccggaaa aagttcaccg ttgatttcga cctgaggcgg gcaattcacc gaaaaattca
     3301 gatcacttac gtgctcgctt tgcttgagca tatggtcaat gattggtttg agattgacgt
     3361 atggcatgga ccagtcttcc attcgggttg ctcaatcgca tcagcagcga ttgagttccc
     3421 tatcctttct tttttcaaat acggcactga ctacaagcca agtcaggttg gtgtggattc
     3481 tgtcgccagt ataatatgcc aatttcacca gcctggaaca cagaaagatc ataactatgg
     3541 agaaatcgaa cactttgctt gaaagtattt tggccggaaa ccatccacac ctggatgagt
     3601 ggatgttttt gctgc
//
LOCUS       DAOQDO010000344         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18925_length_3609_cov_3.082161, whole genome shotgun sequence.
ACCESSION   DAOQDO010000344 DAOQDO010000000
VERSION     DAOQDO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18925_length_3609_cov_3.082161"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2477)
                     /locus_tag="PKZ53_06880"
     CDS             complement(<1..2477)
                     /locus_tag="PKZ53_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40194.1"
                     /translation="MTLDPASSPTLKHFIAESPTRWLTVRYVTALVLIAILGGIGLLV
                     VAAGVDREQQDAHVVNVAGRQRMLSQRLTKLALLLDGTADEALLTTQAAELKRVLDQF
                     ELAHRGLQQGNPDLALLGENSPVVVALYQRISPNFEALRTAGTQLYELTRPAEDWARQ
                     KGERQKLVARILANESPFLSGMDEIVNQYDLEAHQHIAQLKRRFGMLFGGLVVLLILE
                     GLLVFQPAVRRLKALLDSLLEAQLALKRETATIQLLQTITSLANEAQHPEQAISRALE
                     TICTHTGWPLAVVYRVHCANDRFVSSMTWYSSHPTRFFSVQEAIMALRLANRHELVET
                     VSTSEHSRWIARLAESPTWYPVESATSCGLTSAILCPVPVNGRVIAIMELFTASPARP
                     EMSLHATLEQIATQIGLVFDRSDIQTSLIAAKEAAESATQAKTVFLANMSHEIRTPLN
                     GVLGMIQLLVGSPLTVDQRELVRTAQSSGDTLLAIINDVLDFSKIEAGKLTLDSAEFN
                     VHTLLNDVCRMLADNAQNKGLELICLVEPDVEPWWVGDANRLKQVLLNLVSNGIKFTD
                     QGEVIVRTSRVCWPGEPPSLCFEVQDTGIGIDATTQPFLFQPFSQGDGSTSRRFGGTG
                     LGLVISQQLVELMGGQIEFESQPGKGSTFRFTVQLELSTTHDQSVEVEGEILEGKHIL
                     VVDDNATSRTLIQTILSRYGARVQEVESSEQALAFLLAENAPDAQAVDLMIVDQVMPD
                     HSGPELARRLKAEPKLAQIPIVLLTSIGHHRSATSQVLAPANAFLSKPISRAELIQGV
                     TTVLSGPSAVSSGGIHPLELVASPSGAT"
     gene            complement(2603..3241)
                     /locus_tag="PKZ53_06885"
     CDS             complement(2603..3241)
                     /locus_tag="PKZ53_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40195.1"
                     /translation="MTAKVNLVWLHFTAEMVEGFSLVWKAVQWTVIVFVLLHLVLVVT
                     CCLNPMLNWQEKAFLAVGIPCVGAIHGVMVGLVVGGILLGWRLFGWANLVLVVSLLAC
                     TSFSYWVGSHFISGFFDELILTLATGFSQVTTMQAAHISQPIVMILLLPLLLIKFLTL
                     VDAWMALFQLVMAIFVVGLAGVASGLTLSFVPLAWTMLNRFQARSHTQLMTG"
BASE COUNT          877 a          959 c          986 g          787 t
ORIGIN      
        1 gtggcgccag acggtgatgc gaccagttca agcggatgga tgccccctga cgacacggca
       61 gatgggccac tgagcaccgt cgtgacgccc tggatgagtt cagcccggct gattggtttg
      121 gaaagaaagg cattggctgg cgccagaact tgcgacgtcg ccgaccggtg gtgtccgatt
      181 gaagtcagca acacaatcgg aatctgagcc agtttcggct cggccttcaa gcgccgggca
      241 agctcagggc ctgagtgatc aggcattacc tgatccacaa tcatcaggtc cacggcctgt
      301 gcgtcaggag cattctcagc cagaagaaag gccagtgcct gctcgctgga ttcgacctcc
      361 tggacccggg caccataccg ggaaaggatg gtttggatca gggtgcggct ggtggcattg
      421 tcgtccacga ccagaatgtg ttttccctcc aggatttcgc cctccacctc tactgactga
      481 tcgtgggtgg tgctgagttc caattgaacc gtaaacctga aagtcgagcc tttccctggc
      541 tggctttcaa actcaatctg cccgcccatc agttccacca gttgctggga aatcaccagt
      601 ccaagcccgg tgccaccaaa tcggcgactg gtcgagccgt ctccttgcga aaatggctga
      661 aataaaaatg gctgggtggt ggcgtcaatg ccgatcccgg tatcctggac ctcaaaacag
      721 agggatggcg gctcacccgg ccagcagact ctggaggtgc gcacgatgac ttcgccctga
      781 tcagtaaact tgatgccatt gctgaccaga ttgagtaaca cctgttttaa ccggttggca
      841 tcaccaaccc accagggttc gacatctggt tcaaccagac agatcagttc aagccctttg
      901 ttttgggcat tgtcagccag cattcggcag acatcattga ggagggtgtg aacattaaat
      961 tcggcggaat ccagcgtcag tttaccggct tcgatttttg aaaaatccaa aacatcattg
     1021 atgatagcca gcaatgtgtc gccagaggat tgagccgtcc gcaccagttc acgctggtca
     1081 acggtcaagg gtgatccgac cagtaattga atcatgccca gcacgccatt cagcggcgtc
     1141 cggatttcgt gactcatatt ggccagaaag acggtcttgg cctgggtggc actctcggca
     1201 gcctccttgg ccgcaatcaa actggtttgg atgtcactgc gatcaaacac aaggccaatt
     1261 tgggtggcga tttgctccag tgtggcatga agtgacatct cgggtcgtgc tggcgaggca
     1321 gtgaacaatt ccatgatggc aatcacccga ccattgaccg gaactggaca gagaatggcc
     1381 gaggtcaggc cacagcttgt ggctgattca accggatacc aggtgggaga ctcggccagt
     1441 ctggcaatcc acctggaatg ctcactcgtc gagacggttt cgaccagttc gtggcgattg
     1501 gccagccgta aggccatgat tgcttcctga actgaaaaaa agcgcgtcgg atggctgctg
     1561 taccacgtca tgctcgaaac aaagcggtca ttggcgcaat gtacccgata gaccacggcc
     1621 agcggccagc cagtgtgggt acagatggtt tcaagggctc tcgaaatggc ctgttcgggg
     1681 tgttgggctt cgttggccag actggtgatt gtttgcagga gttgaatggt ggcggtttcc
     1741 cgcttcagtg ccagttgggc ttcgagcaaa ctgtccagca gcgctttgag ccgacgaacg
     1801 gctggttgaa aaacgagcag gccctcaagg ataaggagca ccaccagccc accaaacagc
     1861 attccaaacc ggcgtttaag ctgggcaata tgttggtggg cttcgaggtc atattgattg
     1921 acaatttcat ccatcccaga cagaaagggt gactcattgg ccagaatcct ggccaccagt
     1981 ttctgccgtt cgcccttttg ccgggcccag tcttctgcgg gtcgcgtcag ttcatataac
     2041 tgggtacctg ccgtgcggag cgcctcaaaa ttggggctaa ttcgttgata gagtgccacc
     2101 acgaccgggc tgttttcgcc cagtaacgcc agatccgggt tgccttgttg gagcccacgg
     2161 tgggccagtt caaattgatc cagaacccgt tttaattcag cggcctgcgt ggtgagcaat
     2221 gcctcatcag ccgttccatc cagcaagagt gccagtttgg taaggcgttg cgagagcatc
     2281 cgctgccgcc cagccacatt gaccacatgg gcatcctgct gttcccgatc aacgccagcg
     2341 gccaccacca gcaagccgat tccgccgaga atggcaatca gtaccagggc agtcacatag
     2401 cgtaccgtga gccagcgggt cgggctttcg gcaataaaat gtttcagagt tggggaactt
     2461 gcaggatcca gtgtcattga gcctttaggg aattgaaaat aggaggaagt cgctattgtt
     2521 cctgacaggg cgcggaatga caaggtccag tgggaaactc agaggttgca gaaccgatga
     2581 cagggtgaca gggtgtcatt ggttaccctg tcattaattg ggtgtggctc cgtgcctgaa
     2641 accggttcag catcgtccag gccaggggaa caaacgacag tgtcaacccg ctggccacac
     2701 ctgccagtcc aaccacaaaa attgccatta ccagttgaaa tagcgccatc caggcatcaa
     2761 ccagcgtgag aaatttaatc aacaggagcg ggagcagcag aatcatcacg attggctgtg
     2821 aaatgtgtgc cgcctgcatc gtggtcactt gcgagaaccc ggtggcaagc gtcaggatca
     2881 attcatcaaa aaagccggaa ataaaatggc ttcccaccca gtatgaaaaa ctggtgcagg
     2941 ccaacaaact gacaaccagg accagattgg cccacccaaa cagtcgccag ccaagcaaaa
     3001 ttcccccgac aaccagcccg accatcactc catgaatggc accgacacag ggaatgccaa
     3061 ccgccagaaa ggctttttcc tgccagttga gcatcgggtt caggcaacag gtgacgacca
     3121 gcaccagatg gagcagcaca aacacaatca cggtccattg aaccgctttc caaaccagcg
     3181 agaatccttc aaccatttca gccgtgaagt gaagccaaac cagattcact tttgcggtca
     3241 tggattgtat gtggcgaaca gtgtcattga tccaagagaa atacataaca cccgacagcc
     3301 tggaagaatc agaagccttc gaaaaggttt ttttgttgtg gaagatggtg cattgtgccg
     3361 gagatgaatg ggaaatgcac gaagtgaagc gaacttgcag tacctctact catcgaacct
     3421 gcacgttgga tgagattttt ggcgcaagat tgcagagttc aatagatttt cccaatcttt
     3481 ttcaccgcgt ttcttctgac gactgacgac tgactattcc ctgatatcaa ttgagttttg
     3541 accggcggtc tttgattttt agttcctggt agccggattc agggtcattt tcgcgaacaa
     3601 acgtcagat
//
LOCUS       DAOQDO010000345         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18932_length_3608_cov_3.579792, whole genome shotgun sequence.
ACCESSION   DAOQDO010000345 DAOQDO010000000
VERSION     DAOQDO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18932_length_3608_cov_3.579792"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKZ53_06890"
     CDS             <1..488
                     /locus_tag="PKZ53_06890"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40196.1"
                     /translation="NLAVLCNAQARFKDSENYSNRAIKLWTKLVGEDHPNTATAMVNL
                     AFAYASQRKLEKAESLYKRALQIQEKAFGPDHPSVAASLEKLAEVYQLEVRYEDAEKT
                     VRRAMTIWEKKFGPEHPNLANDMEIYSRILRQTNRKESADEFALKAQMLRSRQSDSIT
                     Q"
     gene            complement(598..3288)
                     /gene="aceE"
                     /locus_tag="PKZ53_06895"
     CDS             complement(598..3288)
                     /gene="aceE"
                     /locus_tag="PKZ53_06895"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNJ40197.1"
                     /translation="MYLDDFKRQLPDSDPQETQEWLDALEQITVTQGKERAQFLLRKL
                     LKKARMMDLGGPIPVQTPYINTISPEQEPPFPGDEQMELRIRRMIRWNAVAMVVRANN
                     LNPGIGGHLSTYASSASLYEVGFNHFFRGKDDGQSGDQVFYQGHAAPGIYARAFLEGR
                     LSESQLEHFRREAIPGTGLSSYPHPRLMPEFWEFPTVSMGIGPLTAIYQARFNRYLHA
                     RGLKDTSQSKVWAFLGDGEMDEPESTGALSVAGREKLDNLIFVVNCNLQRLDGPVRGN
                     GKVIQEFESIFRGAGWNVIKVIWSRDWDPIWAQDVDGLFLSRVNSMLDGEFQKISVES
                     GAYIRRHLFGMDERLLKLVEHLSDDQLEKLRRGGHDYNKLFAAYRAAVNHKGQPTVIL
                     AKTIKGWTLGEGLEGRNATHQIKKMNEKELQIFRDLLDLPIPDAKLKDAPFYHPGPKS
                     PEVQYLLDRRQQLGGCVPKRVVRSKPLNLPKKTEYTEFLQKVDQEVSTTMTFVRLLRK
                     LIDDPEIGKRVVLIIPDEARTFGMDFLFKKIGIYAPFGQLYDPVDSNLLFSYRELKDG
                     QILEEGITEAGSMASFTAAGTCYSTHGENLIPFYMYYSMFGFQRTGDQAWAFADARGR
                     GFLIGGTSGRTTLNGEGLQHEDGHSHIQASVIPNILSYDPGFHFEIAVIVREGLRRMM
                     EAQEDVFYYITVHNENYAMPAMPDREGVEEGIIKGLYLFRPSPVERTHRVQLLGSSSI
                     MMQVLRAQEILAEKYDVAADVWSATSYQQLRLEALEVDRWNLHHPESPKRKPYVTQLL
                     EKAPGPIVAASDFVKAWPDMIAPWVPQGFSVLGTDGFGMSDTREVLRRHFEVDAESIA
                     AYALYQLVRQGSIPASVLAQAKNDLGITETDEKGIGHGAD"
BASE COUNT          909 a          952 c          920 g          827 t
ORIGIN      
        1 ataacctggc ggtgttatgc aatgctcagg cacggtttaa agattccgaa aattattcca
       61 atcgggcaat caaactgtgg accaaactgg ttggcgaaga tcatcccaac acggcaacgg
      121 ccatggtcaa cctggctttt gcctacgcct cacagcgcaa gcttgaaaaa gccgaaagct
      181 tatacaaacg cgcgctccag attcaggaaa aagcatttgg ccctgaccac ccaagcgtgg
      241 cggccagcct ggaaaaactt gccgaagtgt atcaactgga agtgagatat gaagacgctg
      301 aaaagacggt gcggcgggca atgacgattt gggaaaagaa atttggaccg gaacatccaa
      361 atctggccaa tgatatggaa atctattccc gaatcctgcg ccaaaccaac cgcaaggaaa
      421 gcgcagatga atttgcactc aaagctcaaa tgctccggtc acgacagtct gacagcatta
      481 cccaatgagt cagtcccact cgcgtgagtg ggtgggtctg gttccttcca acccacccgc
      541 tcacgcaggc ggtactgacc aaacccaccc gctcacgcag gcggtactga caaatttcta
      601 gtcggcaccg tggccgatgc ctttttcatc tgtttcggta atgccaaggt cgtttttggc
      661 ctgggccagg acactggcag gaatgctgcc ctgacgaacc agttgataga gcgcatacgc
      721 ggcgattgat tcggcatcaa cttcaaagtg acgacgcaga acttcgcggg tatcgctcat
      781 tccaaaacca tcagttccca acaccgaaaa tccttgcggc acccacgggg caatcatatc
      841 cggccaggct ttgacaaaat cgctggcggc gacgattgga ccaggtgctt tttcgagcaa
      901 ttgggtgaca tagggtttgc gtttcggaga ttcgggatgg tgcagattcc accgatcaac
      961 ctcaagtgct tccaatcgca attgttgata actcgtggct gaccagacat ctgccgcgac
     1021 gtcatatttt tccgccagaa tctcttgcgc tcgcaggacc tgcatcataa tcgaactgct
     1081 tcccagcagt tgaactcgat gcgtacgttc aaccgggctt gggcggaaca gatacaatcc
     1141 tttaataatg ccttcctcaa cgccttcgcg atccggcatg gccggcatgg catagttttc
     1201 attgtgaacg gtgatgtaat agaagacatc ttcctgtgct tccatcatcc gtcgcagccc
     1261 ttcacggaca atcactgcga tctcaaagtg aaacccagga tcataggaaa gaatgttcgg
     1321 aataaccgac gcctgaatgt ggctgtggcc gtcttcgtgt tgcaagcctt cgccattgag
     1381 tgtggttcgg cctgatgttc caccgatgag gaatccgcgt ccacgggcat cggcaaacgc
     1441 ccacgcctgg tcgccggttc gctggaagcc aaacatcgag tagtacatat aaaacggaat
     1501 cagattttcg ccgtgggttg agtagcaggt cccagcagcg gtaaatgatg ccatcgaacc
     1561 agcttcggtg atgccttctt cgagaatctg gccgtctttt aattcgcgat agctgaagag
     1621 caggttggaa tcaaccggat cataaagctg tccaaacggc gcataaatgc cgatcttctt
     1681 gaataaaaag tccatgccaa aggtgcgggc ttcgtcagga atgatgagta ccacccgttt
     1741 gccgatttcc ggatcatcaa tcaatttgcg cagcaaccgc acaaacgtca tggtggtgga
     1801 aacttcctga tccacttttt gaagaaactc ggtgtactca gtcttcttgg gcagattcag
     1861 tggtttggat ctgaccactc gtttcgggac acacccaccc agttgctgtc ggcggtcaag
     1921 cagatactga acttccgggc tcttcggtcc cggatggtag aacggcgcgt ctttcaattt
     1981 ggcatctgga atcggcagat caagcaagtc acggaaaatc tgcaattcct tttcattcat
     2041 cttcttaatc tggtgggtgg catttcgacc ttcaagtcct tcaccgagcg tccagccctt
     2101 gatggttttg gccaaaatga ccgtaggctg gcctttatgg ttgacggcag cgcgataggc
     2161 ggcaaaaagt ttgttgtagt cgtggccgcc acggcgcagt ttttcaagtt gatcgtccga
     2221 caggtgctcg accagtttga gtaagcgttc atccatgccg aacagatggc gtcggatgta
     2281 agctccggat tcaacagaga ttttctggaa ttcgccgtca agcattgaat tcacgcgtga
     2341 cagaaagaga ccgtccacat cctgagccca gatcggatcc caatcgcgtg accagatcac
     2401 cttgatgaca ttccatccag cgccgcggaa gatggattca aattcctgaa tgaccttgcc
     2461 attgccacga accggtccat ccaggcgctg caggttacag ttgacgacaa agatcaggtt
     2521 gtcgagtttt tcacgcccag ccactgacag ggcgccagtt gattcgggct catccatttc
     2581 accatcaccc aaaaaggccc acactttgct ctgggaggta tctttgagtc cccgggcatg
     2641 cagatagcgg ttgaaccggg cttgataaat ggccgtgagt ggaccgatgc ccatcgaaac
     2701 ggttgggaat tcccagaatt caggcatcag gcgtgggtgt ggataggaag aaagcccggt
     2761 gccgggaatc gcttcgcggc ggaaatgctc aagctggctc tctgacaacc ggccttcaag
     2821 aaaggcccga gcatagatac ccggtgccgc gtgtccttga taaaacacct gatcaccgga
     2881 ttgaccatca tctttgcctc ggaaaaagtg attgaatccc acttcataca gactggccga
     2941 tgaagcatag gtcgaaaggt gtccgccaat ccccggattg aggttgttgg cgcgaaccac
     3001 catggccacc gcgttccacc gaatcatccg gcgaatgcgc aactccattt gttcatcacc
     3061 cggaaacgga ggttcctgtt ccggtgaaat ggtgttgata tagggcgtct ggaccggaat
     3121 cggtccgccc agatccatca ttcgcgcctt tttcagaagc ttgcgcagca gaaactgggc
     3181 acgttcttta ccctgcgtca cagtaatctg ctcaagcgcg tcaagccatt cttgcgtctc
     3241 ttgcggatca ctatcgggaa gctgccgctt aaaatcatca agatacatag agtctctcca
     3301 cttggattag agttcagcct cagttgcggg accttcgggg tgggaacacc aggagaaaag
     3361 gtatggggaa aagcggctca cacggcgaag cccctgcaac tgatgacata tgagtgcgtg
     3421 gccttttgaa tgaaaaatat gcgtagtgcc tggtgcttgg tgcctggtac ttgggaacca
     3481 ataccttcta aggagaaaaa atgaagaacg aagcaccaag aacgaagcac caagaacgaa
     3541 gcaccaaaaa cgaagaacca agaaccaaga aatttcttca ttcttcattc ttcattcttc
     3601 aattctgt
//
LOCUS       DAOQDO010000346         3607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18942_length_3607_cov_3.288007, whole genome shotgun sequence.
ACCESSION   DAOQDO010000346 DAOQDO010000000
VERSION     DAOQDO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_3607_cov_3.288007"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKZ53_06900"
     CDS             complement(<1..1071)
                     /locus_tag="PKZ53_06900"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNJ40198.1"
                     /translation="MSQTVSILGSTGSIGRSTLDVITHLSPRFEVTALSAGNNMAVLA
                     EQIKTYRPQFVAVGSEQAASQLRALIKDDYSGDIGIGMDGMVTVASYPGTECVISATV
                     GGRGLLPTLKAIELGRRVGIANKEPLVMAGELMTRVAQESGAEILPIDSEHNALHQCL
                     RGEKKNEVKRLILTASGGPFRRTPIEEFERLTVAQALKHPTWQMGPKITIDSATLMNK
                     GLEVIEARWLFGTNPDQVEVIVHPQSTVHSMIELIDGSVLAQLGVTDMRHPIQYALTY
                     PERLPANLPALDLVGIGKLEFEVPDRQRFPCLGLAYQALRAGGTMPAVLNAANEIAVA
                     AFLAERIPFIAIPKLIADVMDCP"
     gene            complement(1263..2420)
                     /locus_tag="PKZ53_06905"
     CDS             complement(1263..2420)
                     /locus_tag="PKZ53_06905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ40199.1"
                     /translation="MIKSAEVVIIGGGVIGCSIAYYLTRLGCKDVVILERETFQGKHS
                     TGRSAGGVRQQFATPVNIRMSIFSIDVFSQFKEMTGQDPEYHPFGYLFMATQPEHIEY
                     LHANMKIQQQEGVPVQYVSRDDIREMVPQLFTEDLLGGSFCPTDGFVDPYSVMQGFNH
                     RAREQGARILLETEVTNILTQSGRISGVETNHGTVSTRTVINASGPWARITAGWVGID
                     LPVQPTRRQLVNTEKFDELPMELPMLIDMSNGCYMRKESGGVMIGWADPNEPPSFNTV
                     FDPDFVDVIVEKALPRMPCLENAGINLRRCWGGLYSISPDHHAIVGPVPNLEGFYLCN
                     GFSGHGIMHSPAMGISVAEMIMFGESRTLNVSELKVDRFEKGELLHETAVI"
     gene            complement(2517..2999)
                     /locus_tag="PKZ53_06910"
     CDS             complement(2517..2999)
                     /locus_tag="PKZ53_06910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ40200.1"
                     /translation="MPGFCDSCDERSDRLFDITRVPEFAEVVRSLNYRHVCQACYDDL
                     LEESKHPTPPVDDEERRIEKRLAVHLDVQIEGTSREGIPFREKTTTEDVSLNGAKIIT
                     HQDIEPGSVLKFTVVDSDFETSAIVEVVWKLGVGKTAGLKFVEQNANWKHLLQRQGVL
                     "
     gene            complement(3198..>3607)
                     /locus_tag="PKZ53_06915"
     CDS             complement(3198..>3607)
                     /locus_tag="PKZ53_06915"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNJ40201.1"
                     /translation="TRMQSKKGSGGIYMEQLNQPSLDVSAMYQTLLADFIQVQPDRLK
                     ASDQFIAELRQVTATPTMVEIHPQMNQGDWILIKWPEIRLEVFTYPAKSLATVDLYAP
                     KEIDLDLLFKKLVGFFEPSVIRKTVLSRGILDA"
BASE COUNT          907 a          909 c          875 g          916 t
ORIGIN      
        1 aggacagtcc atcacatcag caatcaactt tggaatcgca ataaaaggaa tgcgctcagc
       61 cagaaaagcc gcgacagcaa tctcattggc cgcgttgaga acagcaggca ttgttccacc
      121 cgcccgaagt gcctgatagg ccagtcccag acagggaaat cgctgccgat ctggtacttc
      181 aaattccagt tttccaatcc ctaccaaatc caatgcgggt aaattggcag gcagtcgctc
      241 tggataggtc agggcatact gaatcggatg ccgcatatcg gtcacgccaa gctgggcaag
      301 gactgatcca tcaatcagct caatcattga atggacggtc gattgtgggt ggacaatgac
      361 ttcgacctgg tcagggttgg tcccaaacag ccagcgggct tcaatgactt ctaacccttt
      421 attcatcagg gtggctgagt caatggtgat tttaggcccc atttgccagg tggggtgctt
      481 taaggcttga gccactgtta atcgctcaaa ctcctcaatt ggtgtccggc gaaaaggacc
      541 accacttgcg gtcaagatca gccgtttgac ttcgtttttt ttctctccac gcagacactg
      601 atgtaaggca ttgtgttcgc tgtcaattgg gaggatttca gctccagatt cctgggcaac
      661 ccgagtcatc aactcccccg ccatcacaag cggctcttta ttcgcaattc caactcgccg
      721 ccccagttca atggctttaa gggttgggag caacccacgt ccaccgacag tggcagaaat
      781 gacacattca gtaccaggat aactggcaac cgtcaccatg ccatccattc cgattccaat
      841 atcgccagag taatcatcct tgatcagggc ccggagctgg ctggctgcct gttcgctgcc
      901 aactgcaaca aactgtgggc gataggtttt gatttgctct gccagcacag ccatattgtt
      961 gccagcactt aaggcagtca cttcaaaacg tggggagagg tgggtaatga catcaagggt
     1021 actccggcca atcgaaccag ttgaacccag tatggaaaca gtttgagaca taagtggtgt
     1081 ttcaggttga ggtgatttga gatgtaagga tggcaaatgt actgactttc agcaccgggt
     1141 gcaaccatcc tgtggtcctc accggcgaaa accagcactg gaatgtcaaa aagggaaatt
     1201 gtttcgtcag accggaaaca acttcccttt cttttcagtg tgcgatgcac tgatttaact
     1261 gatcagatca cagcggtttc gtgtaacagt tcaccctttt caaagcgatc taccttcaac
     1321 tcagaaacat tcagagttcg ggactcacca aacataatca tttcagccac ggatataccc
     1381 atcgcgggtg aatgcataat gccgtgccca ctaaagccgt tgcagaggta aaatccttcc
     1441 aggtttggga ctggaccaac aattgcgtgg tggtcaggtg aaatagaata aagcccaccc
     1501 cagcaccgtc gcagattgat acctgcattt tccaggcagg gcatgcgcgg gagtgccttt
     1561 tcaacgatca catcaacaaa atcaggatca aaaacggtat taaaactcgg tggctcattg
     1621 ggatcagccc agccaatcat tacgccaccc gattctttgc gcatataaca cccattgctc
     1681 atatcaatca gcatgggaag ttccattgga agttcgtcaa atttttcggt attgaccagt
     1741 tgccggcggg ttggttgaac tggcaggtca atgcctaccc atccggctgt aatccgggcc
     1801 cacgggccag aggcattgat cacagtgcgg gttgagacag tgccatggtt ggtctcaacc
     1861 ccactgattc gccctgattg cgtcaaaata ttggtgacct cggtttccag caggatgcgg
     1921 gctccctgtt cgcgtgcccg atgattaaac ccctgcatca cactataggg atcaacaaaa
     1981 ccatcggttg ggcaaaaact cccacctaat aaatcctcag taaagagctg gggcaccatt
     2041 tcccggatgt catcccggct gacatattga actggcaccc cttcctgctg ttgtattttc
     2101 atattggcgt gcaggtattc aatatgctcg ggctgggttg ccataaacag gtagccaaag
     2161 gggtgatact cagggtcctg cccggtcatt tccttaaact gggaaaaaac atcaatggaa
     2221 aagatggaca ttcggatatt gaccggagtg gcaaactgtt gccgaacccc gccggcactc
     2281 cgacccgttg aatgctttcc ttgaaaagtc tctcgttcca gaataacgac atctttgcac
     2341 cctaatcggg ttaagtagta ggcaatacta cagccaatca cacctccgcc gatgataacg
     2401 acctctgctg atttaatcat agagttcctt tgctggtagg tagttttgtt gatggacgaa
     2461 ccgatctggc ttggatcaag ctgccgcggg cccactcagg gccactctgc cccctgctag
     2521 agtacgccct gacgttgcaa gaggtgtttc cagttcgcgt tttgttcgac aaatttcagg
     2581 ccggcggttt ttccaacgcc caacttccac acgacttcga cgatggcaga agtttcaaaa
     2641 tctgaatcca cgacagtaaa ctttaagacg ctgccgggtt caatatcctg gtgggtgatg
     2701 attttggcgc catttaagct aacatcttca gtggtggttt tttcccgaaa tggaattcct
     2761 tcccgactgg tcccttcaat ttgcacgtcc agatgaacgg caagtcgctt ttcaattcgg
     2821 cgctcttcgt cgtcaactgg tggggttggg tgtttgcttt cttccagcaa atcatcgtaa
     2881 caggcctggc atacatggcg gtaattcaag ctccggacga cttcggcaaa ttctgggaca
     2941 cgggtaatgt cgaaaagtcg atctgatcgc tcatcacagc tatcgcaaaa ccctggcata
     3001 gatattctcc gcgtggataa ggttgtggat tcagttaagg agtcaacacg agagtccagg
     3061 ctctatcaga gatcctgacg tacggttgta tggcaaatgg attgaatgcc tgtaaagtga
     3121 ggtggaaagg atattagcag cgtattctca gtggtgaaaa gcaatttttt gaaaccccga
     3181 ttatctcgct caccgagtca ggcgtctaaa attccacgtg aaagaactgt tttgcggatc
     3241 acggatggct cgaaaaaccc aaccagtttt ttgaacaaca ggtcaagatc aatctctttt
     3301 ggggcataca ggtcaactgt ggccaggctt ttggctggat atgtaaaaac ttccagtcga
     3361 atttcaggcc atttgatgag gatccagtct ccttgattca tctgaggatg gatctcaacc
     3421 attgtgggtg tagctgtgac ttgcctgagt tcggcaatga actgatcgct ggcttttaag
     3481 cggtcaggct gaacctgaat aaagtcagcc agcaaggttt gatacatggc acttacatcc
     3541 agggacggct ggttcaattg ctccatatag attccaccac ttcctttttt cgattgcatt
     3601 ctggttt
//
LOCUS       DAOQDO010000347         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_18978_length_3603_cov_3.037204, whole genome shotgun sequence.
ACCESSION   DAOQDO010000347 DAOQDO010000000
VERSION     DAOQDO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18978_length_3603_cov_3.037204"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PKZ53_06920"
     CDS             complement(<1..1287)
                     /locus_tag="PKZ53_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNJ40202.1"
                     /translation="MPYKHKQIRKLAQWGLVVLLVMVGIGWGSTRAYRELTRAETDNL
                     PLTTRVEPQPFTLIIPANGELQAVESTAVVVPNVPVDGPPLRIATIVSEGVFVKKGDV
                     LIEFDPTELQLLVRDNRAVLASTNQKINKGEITASIEKQDLVKEQKIAELELQKLKEF
                     QPKDAEIFTRRQIVEAEIDRAYTEKRIAFAGVRLQLKDKIYSLEEAILMLERQQAETK
                     INDVEKSITSLKLVSPVSGIVVYPDPSMMWGGPLQPGRVVYLGMQLCTVVNPEQMEAK
                     CYVLEKDAGELKPEQPVTVTLDAYPDQHYAGKIKNIHKLARAIERDSPVKYFQVVVGL
                     DQTDQVVMKPGVKLKAQIQAGELKNAIIVPRSALIKRGADFLVYIESQAGKFELAPVK
                     LGQGDQVKVVVTEGLKPGQLIALNPPDIKYRSDSKPV"
     gene            complement(1387..2634)
                     /locus_tag="PKZ53_06925"
     CDS             complement(1387..2634)
                     /locus_tag="PKZ53_06925"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ40203.1"
                     /translation="MPQLSKKRLLFWICLILLMSGGMVASRRFPVFRAKAGNVVEELT
                     MSPRSLSFGVEATGTLRATSVKNFGAPPLFSVYWEMQIATLVPEGTVVKPGDLLVSFA
                     NQQMLNDLQKYQSEVEQANQELEKIKAQNDSQRQELIARLATAESNFEKLKVKQVTGD
                     ATLISVPRDIEKDKLALEQARREVEALNGRLEWQKSSSEAALNIVRSKKSRAETLLTQ
                     IQQRIADLQIKSDRNGVVVYKLKWNGEKFQVGEAAWPGVMVLEIPDLNTLLVDVFVPE
                     IDIARVKPGQRVELTIDAFPGKLYTGKVTQIGTLVRPKAWDVQNKILDVQIQLDQLDT
                     AIMRPAMSIKAKIETRVMEQCLAVPLSAVRTTIDGSLVKVKTEAGWREQNVRLGESNG
                     TEVVIREGLAVGDKVAVEFAKAR"
     gene            complement(2796..>3603)
                     /locus_tag="PKZ53_06930"
     CDS             complement(2796..>3603)
                     /locus_tag="PKZ53_06930"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ40204.1"
                     /translation="EQARIDFQTYANVPKQLVSRRELQEKQLAFEKAKTEFDNQTTKI
                     EQKKKEQAAELRMAEIEVEKLKFQVEQTKLELNQLTVTADTDGIVVYSTFGNERRKFQ
                     VGDTVGWGTSVLQLPDLTRMEAVLNVNEVDGQKLAVGQKTAVVLDSYPDQTFSGTVKE
                     ISQTALKLNPTTTTKIFKTVVSLDQTLPDLMKPGMSARISIEIGTSGTQLMVPRETVQ
                     FQNDVPQVTKVESATQHRPVQITILATDVQTYAIAPNPLLKEGDRLVVGQ"
BASE COUNT          806 a          937 c          874 g          986 t
ORIGIN      
        1 gacgggtttt gaatcagacc gatacttgat gtccggcgga ttgagcgcga tgagctgccc
       61 aggtttcaga ccttcagtta ccacaacttt gacctgatcg ccctggccaa gcttgacggg
      121 tgccaattca aatttcccgg cctgtgactc gatatacacc agaaaatcag ccccacgttt
      181 gattaaagcg ctgcggggaa cgatgatcgc gtttttgagt tccccagcct gaatctgagc
      241 cttgagtttg accccaggtt tcatcacaac ctgatcggtt tgatccaacc cgacaacgac
      301 ctggaagtac ttcactggcg aatctcgctc aatcgcccgg gccagtttat gaatgttttt
      361 gatttttcca gcgtagtgct ggtccggata ggcatcgagg gtcacagtga ctggctgctc
      421 gggtttcaac tctccggcat ccttttccaa tacataacat ttggcttcca tctgctctgg
      481 gtttaccacc gtgcagagtt gcattccaag atacacaacc cggcctggtt gtagtggccc
      541 gccccacatc atacttgggt caggatagac aacgattccc gaaacaggtg aaaccagttt
      601 gagcgacgta attgattttt cgacgtcgtt gattttggtt tctgcctgct ggcgttcaag
      661 catcaggatg gcttcttcga gcgagtaaat tttgtctttg agttggagtc ggacgccggc
      721 aaacgcaatc cgtttttcgg tgtatgcccg atcaatttca gcttccacaa tctggcggcg
      781 ggtaaagatc tcagcatctt ttggctggaa ttctttgagt ttttgtaatt ccagttctgc
      841 aattttttgc tctttgacca gatcctgttt ttcaatcgaa gcggtgattt cccctttgtt
      901 gattttctgg ttggtgctgg ccagcacggc ccgattgtcg cgcaccagca actgcaattc
      961 agttgggtca aactcaatca aaacatcgcc ttttttgaca aaaacccctt cggacacaat
     1021 cgttgcaatc cggagcggag gcccatcaac cgggacgttt ggcaccacaa cggctgtgga
     1081 ttcaaccgct tgcaattcac catttgccgg aatgatcaat gtaaatggct gcggctcaac
     1141 tctggtagtg agtggcaggt tatcggtttc ggcccgagtc agttcgcgat aggcgcgagt
     1201 gcttccccac ccaatcccaa ccatcaccag caggaccacc agcccccact gagccagctt
     1261 gcggatttgt ttgtgtttat aaggcatatg agacataggg acgaaaaaga cgaaaaagac
     1321 gaaaaggacc tacattcgaa aacccggaac ccggaacccg gaacccggaa cccggaaccc
     1381 tacttctcac cgtgctttcg caaattcaac cgcgacttta tcacccacgg cgaggccttc
     1441 gcggatcaca acctcagttc catttgattc accgagcctg acgttctgct cgcgccaacc
     1501 agcctcggtt tttactttga ccagcgagcc atcaatggtg gttcgaaccg cgctcaacgg
     1561 gaccgcaaga cactgttcca tcaccctggt ttcgattttg gctttgatgc tcatggctgg
     1621 acgcataatc gcggtatcaa gttgatccag ttgaatttgc acgtccagaa ttttgttttg
     1681 aacatcccag gcttttggac gaaccagcgt tccgatctgg gtgaccttac cggtgtagag
     1741 tttgcctgga aaggcatcaa tcgtcagctc aacccgttgt ccaggtttca ctctggcaat
     1801 atcaatttcg gggacaaaaa catcaaccag caatgtgttt aaatctggaa tttcaagcac
     1861 catgacccct ggccaggcgg cttcgccaac ctgaaacttc tccccattcc atttcaattt
     1921 gtacaccacg acgccatttc gatctgattt gatctgcaag tcagcgatcc gctgctgaat
     1981 ctgggtgaga agtgtttcgg cccggctctt tttgctgcgg acaatattga gcgccgcttc
     2041 acttgatgat ttttgccatt ccagtcgtcc gttgagtgcc tcaacttcac gccgggcctg
     2101 ttccagtgcc agtttatctt tttcgatatc ccgtggaacg ctgatcaatg tggcatctcc
     2161 ggttacctgc ttcactttga gtttttcaaa gttgctttca gccgtggcca gacgcgcgat
     2221 gagttcctga cgttgagaat cattctgggc tttgattttt tcaagctcct ggttagcctg
     2281 ctcaacttca ctttgatact tctgcaaatc gttgagcatt tgttgattgg caaagctgac
     2341 cagtaaatcc cctggcttga ccactgttcc ttcaggaacc aacgtcgcaa tttgcatttc
     2401 ccaatagacg gaaaacagtg gtggcgcccc gaaatttttg acacttgtgg ctcgaagtgt
     2461 tccggtggct tcaaccccga aactcaacga acgtggcgac atggtgagtt cttccaccac
     2521 attgcctgct ttggcccgaa acactgggaa gcgccgggaa gcaaccatcc caccactcat
     2581 caggagaatc aggcaaatcc agaaaagaag gcgttttttt gacaactgtg gcatggagtt
     2641 aatggcggcc tgggaggaat agaaagaaat tgaaggagcg aaaatcaccg agtcagtacc
     2701 acccgcgtca gtgggtgggt ttgtttgctt ccaaccccac ccgctcacgc aggtggtact
     2761 gacacgttca acaacttcac gacttcgcga tattgctact gaccaaccac cagccgatcc
     2821 ccttctttga gcaatgggtt gggagcaatg gcataggttt gaacgtcagt ggccagaatg
     2881 gtgatttgga ctggtcggtg ttgggttgct gattcaactt tggtgacctg gggtacgtcg
     2941 ttttggaact ggacggtttc acgcggcacc atcaattgag ttccagaggt tccaatctca
     3001 attgagattc gggcagacat cccaggtttc atcaggtcag gaagcgtttg atcaagggaa
     3061 acaaccgttt taaagatttt ggtcgtcgtg gtggggttta atttgagggc ggtttgtgag
     3121 atctctttga ccgtgcctga aaatgtctgg tccggataac tatcgagcac aacggctgtc
     3181 ttttgaccaa ccgccagttt ttggccatca acttcgttga cgtttaagac cgcttccatt
     3241 cgagtcaggt caggcaattg cagcactgaa gtcccccagc caaccgtgtc tccgacttga
     3301 aacttgcggc gttcgttccc aaatgtcgag tacaccacaa tgccatcggt atcagccgtg
     3361 acagttaact ggttgagttc aagtttggtc tgttcgactt gaaatttgag cttttcaact
     3421 tcgatttcag ccatgcgaag ctcagcggcc tgttcttttt tcttctgctc gattttggtg
     3481 gtctggttgt caaactcagt tttggctttt tcaaacgcca gttgtttttc ctgcagctca
     3541 cgccgcgaga caagctgttt gggaacgttg gcataggtct gaaaatcaat ccgggcctgt
     3601 tcg
//
LOCUS       DAOQDO010000348         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19034_length_3598_cov_2.613322, whole genome shotgun sequence.
ACCESSION   DAOQDO010000348 DAOQDO010000000
VERSION     DAOQDO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19034_length_3598_cov_2.613322"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKZ53_06935"
     CDS             <1..715
                     /locus_tag="PKZ53_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40205.1"
                     /translation="PDAAAYVTIQSTEQVPIKDIPPAQERKAKAAVATQVLTTPSTKA
                     IDETTPLSSSTSLLSTETAGPTTTAQPNRWRPAIIGAVLVIGVGGAALGTMSLLSPGS
                     STNGSPSVKPPVATQPVEPATPTTTIPPLPEAGTSPTEPGSGASSSGSSGSTKPAPPV
                     NSNPDTTPETTEVKSEPTTTPPPKPPATSSKPEPVVQKEEKKGFWGKVGGGIKKGLEK
                     IAGKDDDKKKKDDKNKKNK"
     gene            881..2740
                     /locus_tag="PKZ53_06940"
     CDS             881..2740
                     /locus_tag="PKZ53_06940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ40206.1"
                     /translation="MDSAVSSINNGLLGTVLDEKYRLESKIGAGGFGTVYRATHLGLS
                     QAVAVKILQTSITSPSPEDLARFRQEGASTCRIKHPNAVTVYDFGMSSSGFAYLVMEL
                     LEGHSLVQELNAKGPLTPLRAAEILIPVCDVLAEAHAEGVIHRDIKPDNIFLHQTKRG
                     EIVKVVDFGIAKLIDHTTTSGRQGTPTAGLIGTPAYMAPERLRNRPYDGKADVYSLGI
                     TLFQMLTGRLPFEATESGDLAAVILMQVTEDPPCLRDLNPAIPAEVEAVVLKALRKDP
                     TTRPTTAELAHDFANAVGVKIQASSAPMIFSGLSTASDFGMRPTTPAQPHLLIDTEAM
                     TVIAPSTPLELPQTEVILGTPQVQNLETRPNAPAPVKLTDAAGTNLLVSTPSEGAPLA
                     TMPLASSAPLVTRPVQKPDTSSPTPTAVDTLVAVANTRRPVAYKLLLVLLLLAGGLSV
                     MAYRRFFMSEPAPTPPVEIVVPTPPPSPPKPENIQPGGPVLPGEQFPEPDPKEVLGNE
                     HPELPEPGKPPTPAKPPASSGSGKSTTERITVRDGDEVITIDAPKDPPAPPKSNPTVK
                     KNSPQLTPEQREQIRQAKEWQIKSKWLEREIKRQMAIEAQKNRNRRPPDRPNN"
     gene            2868..>3598
                     /locus_tag="PKZ53_06945"
     CDS             2868..>3598
                     /locus_tag="PKZ53_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNJ40207.1"
                     /translation="MTTEQTLPLYIGLDIEGKHLRAAVCDPTGQILTQRRQTVAATTF
                     AELITLVSHTIQDLQAAESDSGTIIGVGFGVPGLVNLQTNQIESWSGFPGLSAVAIYD
                     AILRATQLPIVIDHHSNVAAYGEWQCGVARGFRHIYYVDLGQRMGSSMILNGLMWRGA
                     TGYAGEIGFTTIDLDGERTLESYVSGASIVRRVQKRLHRDRTSSLSRLAIPRDREMEL
                     EDVVNSARAGDEQHGSRQRQANNGD"
BASE COUNT          923 a         1059 c          910 g          706 t
ORIGIN      
        1 tcccgatgca gccgcctatg tcaccattca atcaactgag caggtaccaa tcaaagacat
       61 tcctccagcc caggaacgaa aagccaaagc cgccgtcgcg actcaggttc tgaccacacc
      121 atccaccaaa gccattgacg aaaccacccc cttgagttca tcaaccagtc tcctgagcac
      181 agagacggcg gggcccacca ccaccgccca gccaaaccgg tggcggccag ctatcattgg
      241 cgcggttctc gtgattggtg tgggaggcgc ggctttagga accatgtcgt tgctcagtcc
      301 agggtcctcg accaatggca gcccatcggt taaaccacca gtcgccacgc aaccagttga
      361 accggcaaca ccaaccacca cgatcccacc cttaccggaa gctgggacat ccccgacgga
      421 accgggcagt ggagcgagtt catcaggcag ctctggatca accaaaccgg cgcctccagt
      481 caattccaat cctgatacca caccagaaac aaccgaggtc aaatccgagc ccaccaccac
      541 accgccaccc aaaccaccag ccaccagttc caaaccggaa cccgttgtcc aaaaagagga
      601 aaagaagggg ttttggggta aagtcggcgg cggtatcaag aaaggtctgg aaaaaattgc
      661 aggtaaagac gacgacaaga aaaagaaaga cgacaaaaac aagaaaaaca aatagccgtt
      721 cggctacgtc atttgattgg ctcacatcga actcaaatta ttcccaaagt tttcagtccg
      781 aaagcttcag tttgtcccct tacggttcaa cccaacgact tatgaacctg cagtgcctct
      841 gccacgcgca ggacttcaga caataatcgg taagaacgct atggattctg ctgtatcttc
      901 aatcaataac ggcttgctgg gaacggttct ggacgagaag tatcggctgg aatcgaaaat
      961 cggcgcgggc gggtttggaa ctgtatatcg ggccacccat ctcggactca gtcaggcggt
     1021 ggcggtcaaa atcctgcaaa cttcgattac ctccccttcc ccggaagacc tggcccggtt
     1081 tcgacaggag ggcgcctcaa cctgtcgcat caaacatccg aacgccgtga ccgtctacga
     1141 ttttgggatg tcgtcttctg gttttgccta tctggtgatg gaattgcttg aagggcattc
     1201 actggttcag gagctaaatg caaaaggccc gctcactccg ttacgggcgg ctgagattct
     1261 gattccagtc tgtgatgtgc tcgccgaagc ccatgccgag ggggtgattc accgtgacat
     1321 taagcctgac aatatcttcc tccatcagac caagcgcggt gaaatcgtca aagttgtaga
     1381 ttttggaatt gccaagttaa ttgaccacac caccacctct ggccgtcagg gcaccccaac
     1441 cgctggattg attggcacac ccgcctacat ggcaccggaa cgtctccgca atcgccccta
     1501 tgacggcaag gcggatgtct acagtctggg aatcacgctt tttcaaatgt tgactggtcg
     1561 gttgccgttt gaagccaccg aaagcggcga tctcgccgcc gtgattctga tgcaggtcac
     1621 cgaggatccc ccttgcctgc gcgatttgaa cccagcgatt ccagccgaag ttgaagccgt
     1681 ggtgctcaag gccctgcgca aagatccaac gacgcgccca accacagcgg aactggcgca
     1741 tgattttgcg aatgcggtcg gtgtgaaaat tcaggcgtcg tccgccccca tgattttttc
     1801 tgggctcagt actgcaagcg actttggaat gcgtccaacc actccggctc agccccacct
     1861 gctcattgat accgaagcca tgaccgtgat tgcgcccagt acaccgcttg aactgcctca
     1921 aacagaagtc attttgggca caccacaagt tcaaaacctt gagaccagac ccaatgcccc
     1981 ggcaccggtc aagctcacgg atgcggcagg gacaaacctc ctcgtttcaa ctccctcaga
     2041 aggtgcgccg ctcgccacca tgccgctcgc ctcatcagca ccgctggtca ctcgcccagt
     2101 ccagaaacct gataccagtt cccccactcc aactgccgtg gataccctgg tggctgtggc
     2161 aaacacccga cgaccagtgg cttacaaact tttgctggtg ctcctgttgc tggctggtgg
     2221 cttgagcgtg atggcgtatc gccgtttttt tatgagcgag ccagccccaa caccgccagt
     2281 tgagattgtg gttccaacgc cgccaccgtc cccccccaag ccagagaata ttcaaccagg
     2341 ggggcccgta ctgcccggag aacaatttcc agagccagac ccaaaagagg ttttgggcaa
     2401 cgagcacccg gaattaccag agccagggaa accaccgaca ccggcaaagc caccagcctc
     2461 cagcggcagt ggcaaatcaa caacggaacg cattacagtc cgagatgggg acgaagtgat
     2521 cacgattgat gccccgaaag acccaccggc cccgcctaaa tcaaacccaa ccgtgaaaaa
     2581 gaattcgccg caactgacac cagaacaacg tgaacaaatt cgacaggcca aggagtggca
     2641 aataaaatcc aaatggcttg agcgggagat caagcgccag atggcgatag aagctcagaa
     2701 aaaccggaac cgccgcccac ctgaccggcc aaataactag agtgaaggag tgcatagaat
     2761 ggtgtggcca aataatcaaa accccagagt ttcaggattt gacaggtcag agccacgaat
     2821 gaatgttgaa tgacttcgcg gccttgctac tgttgacgag atttttcatg acgacagaac
     2881 agactttacc gctttatatc ggacttgata tcgaaggcaa acacctccgg gctgcggtgt
     2941 gcgacccaac cggccagatt ctgacccagc gtcggcaaac ggttgcagca acgacgtttg
     3001 ctgaactgat cactctggtc agccatacta ttcaagattt acaggcagcg gaaagcgact
     3061 ctggaacgat tatcggagtc gggtttggtg tgcccggact ggtcaacctg caaaccaatc
     3121 aaattgaatc gtggtccgga ttcccagggc tatcggctgt ggccatctat gatgccatct
     3181 tgcgggccac tcagttgcca atcgtcattg atcatcacag caatgtggcg gcctacggcg
     3241 aatggcaatg cggtgtcgcc cgtggcttcc ggcatattta ctatgtggac cttggccaac
     3301 gcatgggatc ttccatgatc ttaaatggac tgatgtggcg gggtgcgacc gggtatgctg
     3361 gggaaattgg atttaccacg attgatctgg atggagagcg caccctggaa agttatgtct
     3421 ctggtgccag tatcgtccga cgggttcaaa aacgactgca tcgtgaccga acatcatcgc
     3481 tttcacgtct cgccattccc cgcgaccggg aaatggaact tgaagatgta gtcaattcag
     3541 cccgcgccgg cgacgaacag cacggcagcc gacaacgcca ggccaataat ggggataa
//
LOCUS       DAOQDO010000349         3597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19044_length_3597_cov_2.939864, whole genome shotgun sequence.
ACCESSION   DAOQDO010000349 DAOQDO010000000
VERSION     DAOQDO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19044_length_3597_cov_2.939864"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    127..226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(245..703)
                     /gene="bcp"
                     /locus_tag="PKZ53_06950"
     CDS             complement(245..703)
                     /gene="bcp"
                     /locus_tag="PKZ53_06950"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNJ40208.1"
                     /translation="MPNIGDVAPDFSVKDTFGNTVTLSALRGQKVVLYFYPKDDTPGC
                     TKQACSFRDANSIYDEKGIKVLGVSLDDEASHQAFASKYSLPFTLLADTDHAVSEAYG
                     VYGEQEWKGHKFLGVARKTFLIDENGILKKIFDTVNVEAHAQEVLDAFEA"
     gene            complement(900..1544)
                     /locus_tag="PKZ53_06955"
     CDS             complement(900..1544)
                     /locus_tag="PKZ53_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40209.1"
                     /translation="MRTFCLLLFCALFGVGSVTGLTTGSALSASQKPEPHQTTFELPV
                     PPGWKTETIPFPLGFAPELPYRGVEELRFAPGFFKAGQPDFWSYTFVWYLEGNPDFSA
                     DDLSSDLKAYFQGLAKAVEKKEAYTSDKAAVKTDLKKVAASPAGHTLYVGTVANYDAF
                     VTHNRLLLNLKLDVFRCEKEKRKVVVCQLSPQPTTHEIWKTLEQIANGFQCSRP"
     gene            1859..3523
                     /locus_tag="PKZ53_06960"
     CDS             1859..3523
                     /locus_tag="PKZ53_06960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNJ40210.1"
                     /translation="MINTPVEIIPLGGLGEFGMNCLALRYDGSMIIIDVGLMFSEVGH
                     YGVDIMIPDFSYVEEHRDELLAIVLTHSHEDHIGALPFLLQAVNVPVYGTPYTLSVAD
                     RKLDEHKLTDCTQTHTIHAGESFTVGPFTVEFIRVSHSTVDCVALAVTTPVGVVIHTG
                     DYKFDETPVCGDAIDIERLKEYGRRGVLALLSDSTNVERPGRMPSERAVIPALEDIFD
                     QTDGRIFVSCFASSIHRIQIILDLADDFGRSVFPVGRGMLRSLETADQNGFLRIPDGL
                     LLTASEYKQTNSEDVVVLATGCQGEPMAAMSRMANQNHKQAIIEAGDTVILSARQIPG
                     NEKSISRLINECYKRNAFVYDSSSARIHVSGHGAQEDLRLMIESTRPRYFIPIHGEYR
                     QLYRHKLYACQELDYEPENVILMQSGEVLALDGERALISDKIQVGKIYMDDSRSVIVD
                     EAVMRDRKHLAYDGIIIPVLVVNKETGEMKTEPEIATCGYMLLDGNEEWMDELKDVIN
                     QTLTTVPYDERSDMSAFKEKIRLGLKRYIQKLTKQRPTILPVIMQV"
BASE COUNT          865 a          830 c          915 g          887 t
ORIGIN      
        1 ttcatccctc atcccttcaa ttgccccgag cttgcgagtc ttcagcccca agccctgagc
       61 ccaatgtctt ctcagcccca agccctgagc ccaatgtctt ctcagcccca agccctgagc
      121 ccaatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctt cagccccaaa
      241 gcaatcaggc ctcaaacgca tcaaggactt cttgagcgtg ggcttcgaca ttcacagtgt
      301 caaaaatctt cttgaggatg ccgttctcgt caatcaggaa ggttttgcgg gcaacgccta
      361 aaaacttgtg gcctttccac tcctgttcgc catacacgcc ataggcttca gaaacggcgt
      421 gatccgtatc ggccagcaag gtgaagggaa gtgagtattt agaggcaaaa gcctggtgtg
      481 aagcttcgtc atcgagcgaa acaccaagga ctttgattcc tttttcatca tagattgaat
      541 tggcatcgcg gaaactgcag gcttgtttgg tgcagcctgg ggtgtcatct ttgggataga
      601 aatacaacac gactttctgg ccacgcaacg ctgagagtgt aacggtgttg ccaaacgtgt
      661 ctttgactga aaagtctggt gcgacatctc cgatgttggg cattggaaat actcctcgtg
      721 aaaatagatt gaatgagtga aaaaactgga atgccgccag agtattcaca actctgacag
      781 gcagcaagcg attgttcctt gttgggactg aagaagtcag gctgaaaact ttgaagggcc
      841 caatgcgaat gaagcgttga aaaaagaaca gttagaccac tcaaccctct ggtcctcggc
      901 tagggccggg aacactgaaa cccatttgca atctgttcca gggttttcca aatttcatgc
      961 gtcgttggct ggggagaaag ctggcacacc accaccttcc gtttttcttt ttcacagcgg
     1021 aaaacatcaa gcttgaggtt gagtaaaagt cggttgtgcg tcacaaaggc gtcgtaattg
     1081 gccacggttc ctacatagag cgtatgcccg gcgggtgaag ccgctacttt cttgaggtcg
     1141 gttttgacgg ctgccttgtc ggacgtgtaa gcctcttttt tttcgactgc tttggccaat
     1201 ccctggaagt aagctttcaa atctgaggac agatcatcgg cggaaaaatc tggattgcct
     1261 tccagatacc agacaaaggt atagctccag aaatccggtt gcccggcctt gaaaaaacca
     1321 ggggcaaacc gtaactcctc gaccccgcga tacggaagtt ctggggcaaa tccgagcgga
     1381 aagggaatgg tttcagtctt ccagccgggt ggaaccggca attcaaacgt cgtttggtgt
     1441 ggctctggtt tctgactggc tgagagagcg gaaccggtgg tcaagccggt taccgaaccg
     1501 acgccgaaaa gggcacagaa caaaagcagg caaaaggttc tcatagtggt tcttcgtttc
     1561 gggttgaaat ttgattggtt ggtgttcctg acactgtagc gcacaaagca agattgttat
     1621 tggtgaggcg tcaatagagt tgaatgtgtg gttacagcag gtcacgaata tgacttgtac
     1681 gacctgaaaa gttccaaacg gacgcagtat atcgtgcaga ggccggatct tcagccctca
     1741 gcccgacttc ttcagcccaa agtcttccac cccaaaccgg tacttggggt gtcgttagcg
     1801 ccgatgcgtg gtaatatgcc tgtttcgatc aacatgctga aaggatgtcg tttgtatcgt
     1861 gataaatacc ccggttgaaa ttattcctct tggtggttta ggtgaatttg gaatgaactg
     1921 tctggcgctt cgctatgatg gttcaatgat tattattgat gtgggcttga tgttttctga
     1981 agttgggcac tatggggtag acatcatgat cccggatttt tcctatgtgg aagaacatcg
     2041 tgacgaactg ctcgcgattg tgctgaccca tagccacgaa gaccatatcg gagcacttcc
     2101 gtttttgctt caggcggtga atgtgccggt ctatggcacg ccctatacgc tcagtgtcgc
     2161 tgaccgtaag ctcgatgaac ataagctgac ggattgcact caaacccata ccatccatgc
     2221 tggagaatcg tttacggttg gcccgttcac ggttgaattt atccgtgttt cccattcaac
     2281 cgttgattgt gttgcgttgg ccgtaaccac acccgtcgga gtcgtgattc ataccggcga
     2341 ctataaattt gacgaaaccc cagtctgtgg tgacgcaatt gatattgagc gattaaaaga
     2401 atatggccgg cgaggtgtgc tggcattgct ttcagattcc accaacgttg aacggccagg
     2461 gcgaatgccc tctgagcggg cggtgattcc agcgctcgaa gatatctttg atcaaacgga
     2521 tgggcgaatt ttcgtcagtt gttttgcgtc gagcattcat cggattcaga ttattctgga
     2581 tctggccgat gattttggtc gctcggtgtt tccggttggg cgtggaatgt tgcgttcgct
     2641 ggaaacggct gaccagaatg gatttctgcg tattccagac gggcttttgc tcacggcttc
     2701 cgaatacaag caaaccaatt cagaagatgt agtggtgttg gcgactggct gtcagggtga
     2761 acccatggcg gcgatgtcgc gcatggccaa tcaaaaccac aaacaggcga ttattgaagc
     2821 cggggacacg gtcatcctct ccgcgcgcca gattccaggc aatgaaaaat caatttcccg
     2881 attgatcaat gaatgttaca agcgcaatgc ctttgtgtat gactcttcct cggcgcgcat
     2941 ccacgtttca ggtcacggcg cccaggaaga tctgcgcttg atgatcgaat caacccggcc
     3001 acggtatttc atcccgattc atggtgaata tcgccagctc tatcggcaca aactctatgc
     3061 ctgtcaggaa cttgattatg aaccggagaa tgtcattttg atgcagagcg gagaagtcct
     3121 ggcccttgac ggagagcggg cgctcatttc agataaaatt caggtgggca agatttacat
     3181 ggatgacagc cgctcagtca ttgttgatga agcggtgatg cgtgaccgaa agcacctggc
     3241 ctatgatgga attatcattc cagtgctggt ggtcaataaa gaaaccgggg aaatgaagac
     3301 cgagccggaa attgctacct gcggatatat gttgcttgat ggaaatgaag aatggatgga
     3361 tgaactcaaa gacgtcatca atcaaaccct gaccactgta ccgtatgacg aacgttctga
     3421 tatgtcagct tttaaggaaa aaatccggct cggccttaaa cgctacattc aaaagttaac
     3481 caaacagcgt ccgacgattc taccggtcat tatgcaagtc tgatttgtgt ggagtgcggc
     3541 ggcttgacgc cgctttgatt tgaaatgcaa aagcggcgtc aagccgccgc actccaa
//
LOCUS       DAOQDO010000350         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19135_length_3586_cov_2.821580, whole genome shotgun sequence.
ACCESSION   DAOQDO010000350 DAOQDO010000000
VERSION     DAOQDO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19135_length_3586_cov_2.821580"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..820)
                     /locus_tag="PKZ53_06965"
     CDS             complement(371..820)
                     /locus_tag="PKZ53_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40211.1"
                     /translation="MFRNSLMAFALFTLCLGSSHPATAQKSVPNQTIVTAAQVNGTWE
                     TKSGTFKILALGNQQLKVEFQGFYEYKTEYGPMANIGEGRGIAFIEGDTAIFKPEDAS
                     ENDQITLKFTKGKLIVTQEGSCGFGLNVTAAGTYRKVSSQKPKFETE"
     gene            complement(893..2356)
                     /locus_tag="PKZ53_06970"
     CDS             complement(893..2356)
                     /locus_tag="PKZ53_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40212.1"
                     /translation="MVQQQFMVQTTWRCLVALMLVWLGNGTIRAQTEFTPGHIQPKVV
                     CQENADHSYALYLPKAYTKDKKWPIVYAFDPGGNGSAPVELLQEAAEDLGYIVVGSNN
                     SHNGLNVPISEIVRIFWRDTRQRLSFDEKQIYTAGMSGGCMVATAIARLSKGEISGTF
                     LVGSPRIPELLESKQLPFIVFGSAGTSDFNYFHVRDIIETLQKNSIPSTFVVFDGTHG
                     WCDKQTARSALEWFDLQAMKTGRKPKNEAWLTAFYEKQIATARELETAGMSGAAYWKL
                     VNLNRDYAGLTPTPELDSKIQALKNTKPVQQWFKDEVDLRRRHSAKMEEFFGHYHGLF
                     DEEGQFESFSAMRSLISPLKKQAADRTPSVSRQVATMVLDHCFIMMFEESMRYFSTSE
                     YRKAITCLEIDADIRPTDQGPWYYIACAYAEMHDAKRAVTALKKVVELGPVRRAVIEK
                     NKHFDPIAKEEAFQKWLASLGQPASEATEKDGAKADK"
     gene            complement(2593..2955)
                     /locus_tag="PKZ53_06975"
     CDS             complement(2593..2955)
                     /locus_tag="PKZ53_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNJ40213.1"
                     /translation="MKLYVGNLSFKTVEEDLSQLFAQAGTVDSATVVVDRETGRSRGF
                     GFVEMASKEDGMRAISQFHGTELDGRTLTVNEARPREERGGGNRGGGFGGGGHRGGGG
                     GHRGGGGGGGHRGGGSRW"
BASE COUNT          915 a          926 c          837 g          908 t
ORIGIN      
        1 atctaccaac cgtcggccag ccaatttcga cccatccaac tgaaaccttc atcacgccca
       61 aaccgctaac cgatcagcaa cttgcagatg cctcgacggt ccagatttcc aaaactgcac
      121 tgaccgatga aatttcaact gatccgccag ggaatgtgtg acaacccaag aaaaatgtct
      181 gggaccagtt aacatatttt gtttctttga cttaacccgt tgtgaacgcg ttcacaaatt
      241 gttttcacat cgccctttgg tagcctgacc tgggcctgta ttttgctgtc gagtcacagg
      301 ggtgactctg ttgttgaggg agattgactt ccctcatctg gcaaagtgtt cggtcctggc
      361 cttttggttg ctactcagtc tcaaatttgg gtttctgact gctgactttg cggtaggttc
      421 cggcggctgt gacattgagt ccaaacccac aactgccttc ctgagtaaca atgagcttgc
      481 ctttggtaaa tttgagcgtg atctggtcgt tttcactggc atcttcgggc ttaaaaatag
      541 ctgtatctcc ttcgataaag gcaattccac gtccttcacc aatattggcc attggcccat
      601 attcggtttt gtattcataa aatccttgaa attcaacctt gagttgctgg tttcctaagg
      661 caaggatctt gaaggtgcca cttttggttt cccaggtgcc gttgacctga gccgccgtga
      721 caatcgtttg gttgggaaca gatttttgcg ccgtggccgg gtgacttgat ccaaggcaca
      781 gggtaaacaa cgcaaatgcc atcaaagagt tacggaacat aaagaaagtc tctcctttaa
      841 aaacattgaa ttcttcctcc aaccgccgcc cttgagttta ctttcttcgg cgctacttat
      901 cagccttggc tccatctttt tctgttgctt cagacgcagg ttgacccaat gaagccagcc
      961 atttttggaa agcttcttct ttggcaatcg gatcaaagtg tttgtttttt tcaatcaccg
     1021 cccggcgcac ggggccaagc tcaaccactt ttttcagcgc cgtgaccgct cgtttggcat
     1081 cgtgcatttc agcataggca caggcgatat agtaccacgg cccctggtca gttggtcgga
     1141 tatcagcatc aatttcaagg caggtaattg cttttcgata ctcgctggtc gagaaatacc
     1201 gcatgctttc ttcaaacatc atgataaagc aatgatccag gaccatggtt gccacctgcc
     1261 gcgaaacaga tggagttcga tcagccgctt gtttcttcaa tggggaaatc aaggaacgca
     1321 tagcggaaaa gctttcaaat tgcccttcct catcaaataa tccgtgataa tggccaaaaa
     1381 actcttccat tttggcgctg tgtcggcgac gtaaatcaac ttcatcctta aaccattgct
     1441 gaaccggttt ggtatttttc agcgcctgaa tttttgaatc aagctctgga gttggggtca
     1501 ggccagcata gtcgcggttg agattgacca gcttccagta agcagcccca ctcatcccag
     1561 ccgtttccag ttctctggct gtggcgattt gcttttcata gaaggccgtc aaccaggctt
     1621 cgtttttagg tttgcgaccc gttttcatgg cctgaagatc aaaccactcc agggcactcc
     1681 gggcagtttg cttgtcgcac cacccatgcg taccgtcaaa caccacaaag gtactgggga
     1741 ttgaattctt ctgcagggtt tcaatgatgt cccgtacgtg gaaatagtta aaatcacttg
     1801 taccagccga gccaaagaca ataaacggaa gttgtttact ttccaaaagt tctggaatcc
     1861 gtggcgaacc aacaagaaag gtacctgaaa tttcgccttt actcaggcgg gcaatcgccg
     1921 tggctaccat gcatccacct gacatgccgg cggtataaat ctgcttttca tcaaatgaaa
     1981 gtcgctgtcg agtatcgcgc cagaagatcc ggacaatctc tgaaataggg acattgagtc
     2041 cattgtgcga gttgtttgat ccaaccacaa tatagccaag gtcttcggca gcttcctgga
     2101 gcaactcaac cggggccgaa ccatttccgc caggatcaaa agcatagaca atcggccatt
     2161 ttttgtcttt tgtgtaggct ttcggcaggt agagcgcata actgtggtca gcattttcct
     2221 ggcataccac tttgggctgg atatgcccag gcgtaaattc agtctgagcg cggattgttc
     2281 cattccccag ccacaccagc atcaacgcca ccaggcacct ccaggtggtt tgaaccataa
     2341 attgttgttg aaccataaac ccctttttct ccaagcaaaa gatgcacccg gcctgagatg
     2401 atgattggta tctggcactt taaaggaaaa gaaggatcgg gaaaaaggaa aagtgatcag
     2461 ccggcaggca gggatgatga cccggtgatc gcagtatttg gaaaaacagc cccaaaacaa
     2521 caaccccctg attgtgtgac cacaaccagg gggttgaata tttggacgat caggggcgat
     2581 cagatggaaa aattaccagc gtgatccgcc accacgatga ccgccgccac cgccgccgcc
     2641 gcgatgacca ccaccgccgc cacgatgacc gccgccgccg aatccgccgc cacgattgcc
     2701 accgccgcgt tcttcacggg gacgggcttc gttcacggtc agggtccgac catcgagttc
     2761 ggtgccatgg aactgggaaa ttgcgcgcat accatcttct ttggaggcca tttcgacaaa
     2821 accaaaaccg cgtgagcggc cagtttcacg atcaaccacg acagtggcgc tatcaacggt
     2881 accagcctgg gcgaacaact ggctcagatc ttcttcaacg gttttaaagg acagattgcc
     2941 aacgtagagc ttcatagact taaattctca ccattctaaa gaggagcagg aagcgaaatc
     3001 caagtgggtt cggtgaatct gacagcaaac aaaacttgct ttgaaaagta cccagagagg
     3061 cttctgcagg ttatgaccac cctctcaaac aatcccaacc taccgccagt tcacccaaac
     3121 aagaagctac gaggcaaatc accgaaggcc gaatgagagg aaaaaagaac tcggcgcagt
     3181 atgggggaag aattccaaaa aggcaagcat ctgttgcaaa aaatttgtgg gtttattctt
     3241 ccaaaatact tcttcccatt tgagattgac acgagtgcgg ccaattcaaa ctcaaatttg
     3301 ggaaaacgac ccagattgcc aaacccgtgc gggcctgttc ggtgtgaaac ccggtgtttt
     3361 tgaacgtctg aacaaatttt gacatccagg ttgagccgct catacactga accccctcga
     3421 tgaaaatcat gaggaggccg gcagttgggt accctcgaac cccaatcacg ccaggaatta
     3481 tcgtgattta aagctgacag tcatgtacag accagtgacg tgtatcggtt tgagcctgtc
     3541 cagcctgctt tggtatggtt tttggggatt gtctggttta ctgttt
//
LOCUS       DAOQDO010000351         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19140_length_3585_cov_7.584136, whole genome shotgun sequence.
ACCESSION   DAOQDO010000351 DAOQDO010000000
VERSION     DAOQDO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19140_length_3585_cov_7.584136"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PKZ53_06980"
     CDS             complement(<1..1306)
                     /locus_tag="PKZ53_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ40214.1"
                     /translation="MQKLAEICVNRPVFATMLILALTVAGAFSYFSLGVDRFPKVDFP
                     VITVTVANPGSSPAEIETEITDKIEAAVNTISGIKELRSTSVEGVSQVFIQFNLDKNF
                     DVAAQEVRDKVNLVISDLPNTIETPTVQKFETNSSPVMQVAVAAPRPLVQVTDIAKKL
                     IKERIENIPGIGQVRIIGGNKREIKVLVNPDRMRAYGVSAVEVVEAVRRQNLEVPGGR
                     IPQGSQEFTLRTLGKLLEVPDFETIPVIQRGDFVVRLRDIATIEDGTEEQRSLSLLNG
                     QPAVTLLISKRSGENTVAVIGAVKERLEEIRPTLPSDVTFQIVSDQSVFVEAAVNDVK
                     THLFEGSLLACLIVFLFLWRFGLTLIAGIAIPISIIGTFALMAAFGYTLDIITLLALT
                     LMVGVVIDDAIVVLENIERFIREKGFAPFPAAIEATREIGLAV"
     gene            complement(1530..2837)
                     /locus_tag="PKZ53_06985"
     CDS             complement(1530..2837)
                     /locus_tag="PKZ53_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ40215.1"
                     /translation="MKKKSAYSLFGALGLGFFCLVGLAGCQGKASTPTRADAAPSAPL
                     MVNVKPVFQRTLPTYLETTGSISAIQSSKVAANATGLIAEIPIQSGDFVKEGATLARL
                     DPREANLRLDQAKAGVAQAEAALKQAESRLGVTGDTAFDPMKMAEVASAQANLDAAES
                     AVRLAESNFKRYEKLAQNDNIPRVQFDQARNQVETARANATAAQRQLDVAIKVARQEF
                     QSVAAARASLEQARLQVALAEKSRNDLTIRSPFSGFVVERLSNQGEYVMPDTPVAMIV
                     RHDPIKVQLQISETQLAKVKIGQEVSLASDAFPDRKFSAKVTALNPIVDQNARSLMIE
                     AQMANPDGVLRPGMFVKARVAQPEQRTALFVPVKAVFYQPALNASGVYVIEGTKARLR
                     IVQPGSRDGEELEILTGLTPEDQVVVAPPAKLIDGSLVEIQSK"
     gene            complement(2964..3563)
                     /locus_tag="PKZ53_06990"
     CDS             complement(2964..3563)
                     /locus_tag="PKZ53_06990"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ40216.1"
                     /translation="MEKPPRKSRAQIATAAANTKVAIVQAAQQIVVRDGVNALTIDAV
                     ARESGVSKGGVLYHFKTKEALIQGMVDSLCSEYEAILLNHRETEGDVPGSWARAYIKS
                     NLDPANASQENFSVGAAILAAVANNHELVGPFREKLHDWYELMAQDSPTPTPALIAAL
                     ASDAMFFFKMLGLWTMSQEQQDNLVAAMLELTQGDRVTE"
BASE COUNT          872 a          934 c          936 g          843 t
ORIGIN      
        1 gtacggccag cccaatttca cgggtggctt cgatggccgc cggaaatggc gcaaagcctt
       61 tttcacggat gaatcgctca atgttttcaa gcaccacaat cgcatcgtca atcaccacgc
      121 ccaccatcag cgtcaatgcc agcaaggtaa tgatgtcgag cgtgtatcca aaggccgcca
      181 tcagggcaaa ggttccaatg attgaaattg gaatggcaat cccggcaatg agtgtgagcc
      241 caaatcgcca caaaaacaga aagacaatca aacaggcaag caggctgcct tcaaaaagat
      301 gggtcttgac atcattgacg gcagcttcga caaaaaccga ctgatcagaa acaatctgaa
      361 aggtgacatc agagggcagg gttggacgga tttcttccaa tcgctctttg acagcaccaa
      421 ttacggcaac ggtgttttca cccgaacgtt ttgaaatcag aagggtgacg gctggctgtc
      481 cattgagcag cgagagcgac cgttgttctt cggtaccgtc ttcaatcgtg gcaatatcgc
      541 gcagtcgcac cacaaaatcg ccgcgctgga taaccggaat cgtttcaaaa tcaggtactt
      601 ccagcagttt tcccagggtg cggagcgtaa attcctggct tccttgagga attcggccac
      661 ctggaacttc aagattctgg cgacgaaccg cttcgacaac ttcgacggct gagaccccat
      721 aggctcgcat tcggtctgga ttgaccagca ctttgatctc gcgcttgttg ccgccaatga
      781 tgcggacctg cccaattccg ggaatgtttt cgatccgttc cttgatcaac tttttggcga
      841 tgtcagtcac ctgcaccagt ggtcgaggtg ctgcgaccgc cacctgcatc actggcgagg
      901 agttggtttc aaacttctga accgtcggcg tttcaattgt gtttggaaga tcagaaatta
      961 ccaggttgac cttgtcacga acttcttgcg ccgcaacatc aaagtttttg tccagattga
     1021 actggataaa cacctgggac acgccttcaa ccgaagttga ccgtaactct ttgattccac
     1081 tgatggtatt gacggcagct tcaatcttgt cggtaatttc ggtttcgatc tcagccggag
     1141 atgatcccgg attggccact gtcaccgtga tgaccggaaa atccaccttc gggaagcgat
     1201 ccaccccgag tgaaaagtaa gaaaacgcac cggccaccgt cagtgccagg atcagcatgg
     1261 tggcaaaaac cgggcgattg acgcagattt cggctaattt ttgcatagca agataccaat
     1321 tcgggttcag agttcagggt tcagggttca gggttcaggg ttcagggtat gaaaaagaca
     1381 aaaaggacat aagggacgaa aaggacgaaa agggaacaac gtgaactaaa ggcactcttg
     1441 ccttgaacct tgccagtttt cgaaaacccg gaacccggaa cccggaaccc ggaacccgga
     1501 acccggaacc cggaacccgg aaccctgtat tatttcgact gaatttcaac cagtgacccg
     1561 tcaatgagtt tggcgggcgg ggccacgaca acctgatcct ccggtgtcaa cccggtcagg
     1621 atttcaagtt cctctccgtc gcgggaacca ggctgcacga ttcgaagccg ggctttagtc
     1681 ccttcaatca catacacccc agaggcgttg agtgctggct ggtaaaacac cgctttgacc
     1741 ggtacaaaca gcgcggtgcg ttgttcgggt tgcgccacgc gggctttgac aaacataccg
     1801 ggacgcaaca cgccgtcggg gtttgccatt tgggcttcaa tcatcaggga gcgggcgttc
     1861 tggtcaacga ttgggttgag tgccgtgact ttggcggaga atttccggtc tggaaatgca
     1921 tcgctggcca gggatacttc ctggccaatt ttgactttgg caagctgggt ttctgaaatc
     1981 tgaagctgaa ctttgattgg atcatggcgc acgatcatcg ccaccggagt gtccggcatg
     2041 acgtattcgc cctgattgga aagccgctcg acgacaaacc cggaaaaggg tgatcgaatc
     2101 gtcaggtcat tgcggctttt ttcggccagt gcaacctgta gccgggcctg ctcaagtgag
     2161 gcacgggcgg cagcgacaga ttgaaactct tggcgagcca ctttgatggc gacgtccaac
     2221 tgtcgctggg ctgctgtggc attggcccgc gcggtttcaa cctgatttcg agcctgatca
     2281 aactggacgc ggggaatgtt gtcgttttgg gccagttttt catagcgttt gaaattggac
     2341 tcagccagcc gaacggcact ttcagccgcg tcgaggttgg cctgggccga agcgacttca
     2401 gccattttca tcgggtcaaa tgctgtatca cccgtcacgc ccagccggct ttcggcctgc
     2461 tttaaggcgg cttcagcctg tgccacgcca gctttggcct gatcaagccg aagattggct
     2521 tcgcgtggat caagtcgagc cagggtcgcc ccttctttga caaagtcgcc cgactgaata
     2581 gggatttccg caatcaatcc agtggcattg gcagcaactt tcgaactttg aatcgcagaa
     2641 atgcttcctg ttgtctccag ataggttggg agcgttcgct gaaaaaccgg ctttacatta
     2701 accatcaacg gagccgaagg tgctgcgtct gcacgggttg gtgtggaggc tttcccctga
     2761 catccagcca gtccgacaag gcaaaaaaag cccaatccaa gtgctccaaa aagtgaataa
     2821 gcagattttt ttttcatatt ctttcaatgt accgtccaga cggtttgtaa aaaaatgaag
     2881 acagttaccg aatcaaaaga atgaagaatg aagaatgaag aatgaagaat tgtcactctg
     2941 tcatctggtc atttttcaca ttgtcactct gtcactctgt ctccttgggt cagctccaac
     3001 atagccgcca caaggttatc ctgttgttcc tgagacatcg tccacaggcc gagcatttta
     3061 aaaaagaaca tcgcatctga cgccagggct gcaatcaacg caggagttgg tgtcggactg
     3121 tcctgggcca tcaactcgta ccagtcgtgc aatttttccc gaaatgggcc aaccagttcg
     3181 tgattattgg cgacagccgc cagaatcgca gcgccaactg agaaattctc ctgggacgca
     3241 ttagccggat cgagatttga tttgatatag gcccgtgccc acgaaccagg cacatcacct
     3301 tcggtttccc ggtgattgag caaaatagcc tcatactcgg aacacaagga atcaaccatc
     3361 ccctggatca gggcttcctt ggttttaaag tggtagagaa cgccaccttt actcacgccc
     3421 gattcacgtg ccacagcgtc aatcgtcagc gcgttgaccc cgtcacgaac aacgatttgt
     3481 tgagctgcct gcacaattgc aactttggtg ttggcagcgg cggtggcaat ttgagcgcgt
     3541 gattttcggg ggggtttttc catggatttt caattttttg gcaag
//
LOCUS       DAOQDO010000352         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19154_length_3584_cov_3.184755, whole genome shotgun sequence.
ACCESSION   DAOQDO010000352 DAOQDO010000000
VERSION     DAOQDO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19154_length_3584_cov_3.184755"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /gene="hypD"
                     /locus_tag="PKZ53_06995"
     CDS             complement(<1..450)
                     /gene="hypD"
                     /locus_tag="PKZ53_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNJ40217.1"
                     /translation="MKYLDEFRDPELAQSILQEIKARVTRHWAIMEVCGGQTHSIIRN
                     GIDQLLPPEIELIHGPGCPVCVTPLEMIDKALAIAARPNVIFCSFGDMLRVPGSDKDL
                     FRIKSEGGDIRIVYSPLDAVKLARDNPGREVVFFGIGFETTAPANAMA"
     gene            complement(598..867)
                     /locus_tag="PKZ53_07000"
     CDS             complement(598..867)
                     /locus_tag="PKZ53_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNJ40218.1"
                     /translation="MCLGVPGKIVEIYDAGNVRMGRVDFEGVVKEVCLAYLPEIEVGD
                     YTIVHVGFAITKIDEKSAQETLRLFYEMGILEEAIGQEEGSGFRV"
     gene            complement(1008..3368)
                     /gene="hypF"
                     /locus_tag="PKZ53_07005"
     CDS             complement(1008..3368)
                     /gene="hypF"
                     /locus_tag="PKZ53_07005"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNJ40219.1"
                     /translation="MPTPPASIAAALARRRLFVTGVVQGVGFRPFVYTCAIRNQLTGF
                     VGNDSQGVFIEIEGSIADLDLFQSELTLKKPPLAHIEDISAIDIPAMGSSGFQIVESH
                     STTAVKTLVSPDICLCDDCLAELEDPTNRRYRYPFINCTNCGPRFTIIKSLPYDRPAT
                     TMAGFVMCPACQSEYDDPTDRRFHAQPNACPECGPHVEYFDIQDPDLIASSDAAMYRT
                     QQALAEGRIMAIKGIGGFHLACDARNNQALETLRSRKGRIDKPFAVMARSLEVIRQFA
                     EVSDDEQALLTSRERPIVLLRHKSGSNLSPLIAPGNEYIGVMLPYAPLHFVLMTSVKG
                     GPSVPEVLVMTSGNFSNEPIVTANSQAVIRLADMADAFLVHNREINVPCDDSVVRVFQ
                     KAELPIRRSRGYAPFPVKLPFSTDPILAVGGELKSTFCLTKDNYAILSQHIGDMENLE
                     TLQVFEQSVTHLTTLFQVEPQAVAYDLHPGYLSTKWAGRYVEHSAESPKTLVGVQHHH
                     AHIAAALAEHQWNGTSPVIGFSFDGTGYGLDGAIWGGEVLLANYKGFQRAAHLRYVPL
                     PGGDVAVRKPYRCALAHLWAAGVEWNEALPPVQECSLVERNAVLRQLQTGLNAVATSS
                     MGRLFDAVASLAGVRHRVTYEAQAAIEFEALTNVKIEGEYRFDLIESEPIQIDPTPAI
                     REIARDVRTGFPVELIGGKFHKGVASLILNLARHFRKTTHLTQVALSGGVFQNTTLLE
                     LVTSLLHNDGFEVFTHSQVPPNDGGLALGQAVIAHMVGAEKTRARG"
BASE COUNT          908 a          934 c          928 g          814 t
ORIGIN      
        1 cgccatggcg ttggctgggg ccgtggtttc aaaaccaatg ccgaagaaaa cgacttcccg
       61 gcctggattg tcacgggcaa gtttcacggc atccagcggt gaatacacaa tccggatgtc
      121 gccgccttcg cttttgattc gaaaaagatc tttgtcactc cctggcacac ggagcatgtc
      181 gccaaacgag cagaaaatca cgttcgggcg ggcggcaatt gccagagcct tatcaatcat
      241 ttccagtggt gtgacgcata ccggacagcc gggcccgtga atcaactcaa tttccggtgg
      301 gagcaactgg tcaattccgt tgcgaataat cgaatgcgtc tgcccgccac acacttccat
      361 tatcgcccag tgtcgagtga cacgggcttt gatttcctga aggatgctct gggccagttc
      421 tgggtcgcga aattcatcaa ggtatttcat aggctggggc tcaggggata gggcttgggg
      481 gttggggttc aggaatagaa aaagacagaa ataacgaaag tgactaaaag gacgtaaatt
      541 cgaaaacccg gaacccggaa tccggaatcc ggaatccgga accctggttt ccaaacccta
      601 tacccggaac ccggaaccct cttcctgtcc aatggcttct tccaaaatgc ccatttcgta
      661 aaacagacgc agcgtttcct gggcggattt ttcatcaatt ttggtaatcg caaacccgac
      721 gtgaacgatg gtgtagtcac caacttcgat ttcaggaagg taggcaagac aaacttcttt
      781 gacaacacct tcaaagtcta cgcgacccat acggacattg ccagcatcgt aaatctcgac
      841 gatttttccc ggaactccaa gacacatgcg atgagtcctt tcttttctta gtgatgattt
      901 aaagttgaga ggtgagactc aagaaagggc aaaaaggatc gaaaggatga aagggatata
      961 aacttgaaga gcaatggttt tctcctttaa ccctcagccc gatgtcttca gccccgagcc
     1021 cgagtttttt cagccccaac catatgagca atcactgctt gtccaagcgc caatccacca
     1081 tcgttgggcg gcacctgaga gtgggtaaag acttcaaaac catcgttgtg cagcagactg
     1141 gtcaccagtt caagcagcgt tgtgttttga aacacgccgc cgctgagggc tacctgggtc
     1201 agatgggtgg tttttcgaaa atgccgggcc agattcagaa tcaggctggc aacgcctttg
     1261 tggaattttc cgccaatcag ttcgacagga aacccggtcc gcacatcccg ggcaatttcg
     1321 cggatggccg gagttgggtc aatttgaatc ggttcgcttt cgatcaagtc aaaccggtac
     1381 tcaccctcaa tcttaacatt ggtaagggct tcgaattcaa tggcggcctg tgcttcgtag
     1441 gttacccggt gacgaacgcc agccagcgaa gccactgcgt caaaaagtcg gcccatgctg
     1501 ctggttgcga cggcgttcaa tccggtttgg agttgtcgca gaaccgcatt tcgttccacc
     1561 agcgagcatt cctgaactgg tggcaaggct tcgttccatt caactccagc agcccacaaa
     1621 tgggcaagtg cacagcgata gggtttccgt acggccacat ctccaccagg aagcggcaca
     1681 tagcgcaggt gcgccgcccg ttgaaatcct ttgtagttgg ccagcaggac ttcaccgccc
     1741 caaatcgcgc catccagccc atatcctgta ccgtcaaaac taaagccaat caccggggat
     1801 gtaccattcc actggtgctc ggcgagcgca gcggcaatgt gagcatggtg gtgttggacg
     1861 ccaaccaggg tttttggcga ttcagcggaa tgctcgacgt atcgcccggc ccatttggtc
     1921 gaaagatacc ccggatgcag gtcataagcg accgcctgag gttcaacttg aaacaacgtc
     1981 gtcaggtgag tcaccgactg ttcaaagacc tggagtgttt cgaggttttc catgtcgcca
     2041 atgtgctgac tcaaaatggc gtagttgtct ttcgtcaaac agaaggtgct tttcaattca
     2101 ccaccaaccg ccagaattgg atccgtcgaa aatggcaatt tcaccggaaa cggtgcatat
     2161 ccacgcgacc gacgaatggg caattccgct ttttgaaaca cccgcaccac tgaatcatca
     2221 cagggcacat tgatctcgcg attgtgcacc agaaaggcgt cggccatgtc cgcaagtcga
     2281 atcactgcct ggctattggc ggtgacaatt ggttcattcg aaaaattccc cgaggtcatc
     2341 accagcactt cgggtactga cggcccacct ttgacggaag tcatcaggac aaaatgaagc
     2401 ggcgcatatg gcagcatcac gccgatatat tcatttccgg gtgcaatgag cggtgacagg
     2461 ttgctaccgg atttgtgtcg caagagcaca atcggacgtt cccgactggt cagcagtgcc
     2521 tgctcatcgt cggaaacttc ggcaaattga cgaatgacct ccagcgagcg agccatcaca
     2581 gcaaatggtt tatcaatgcg gcctttccgg ctgcgcaagg tctcaagcgc ctgattgttt
     2641 cgagcgtcac aagccagatg aaagccgcca atgcctttga tcgccatgat gcggccctca
     2701 gctagtgcct gctgggtgcg atacatggcg gcatcgctgc tggcaatcag gtctggatct
     2761 tgaatatcga aatactcgac gtgcggccca cattcggggc aggcatttgg ctgggcatga
     2821 aacctgcggt cagtcgggtc atcatattca gattgacagg ccgggcacat cacaaaaccg
     2881 gccatcgtgg ttgctggccg gtcatacgga agggatttga taatggtaaa ccgggggccg
     2941 caattggtgc agttgataaa tggatagcga tatcggcgat tggtagggtc ttcgagttct
     3001 gccagacagt catcgcacag gcaaatatca ggtgacacca gtgtttttac ggcagtggtc
     3061 gaatgacttt cgacgatttg aaacccggaa ctccccatgg ctggaatatc tatcgccgaa
     3121 atgtcctcga tatgagccag tggtggtttt ttcagcgtca attcactctg aaacagatca
     3181 agatcagcaa tcgaaccctc aatttcaata aaaacgccct gactgtcatt gccgacaaag
     3241 ccagtcaact gattgcggat tgcacaggta tacacaaacg gtcgaaaccc aacaccctgt
     3301 accacgccag tcacaaaaag tcggcggcga gcaagagcgg cagcgatgga agcaggtgga
     3361 gtcggcatag ggttttaggg ttcagggttt agggttcagg gttcagggtt cagggttccg
     3421 aatctttaat gagatgtgaa aaagtagaat gattgggtct tgaggttgat tatctaaagg
     3481 ttgctcaaca tcttacgaat gaaaagcaaa ttattcaaac tgcctttggg taaaccccaa
     3541 accctgaacc cccaaccccc aacccctatg cttcacgtta ccaa
//
LOCUS       DAOQDO010000353         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19163_length_3583_cov_2.775510, whole genome shotgun sequence.
ACCESSION   DAOQDO010000353 DAOQDO010000000
VERSION     DAOQDO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19163_length_3583_cov_2.775510"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PKZ53_07010"
     CDS             complement(<1..1296)
                     /locus_tag="PKZ53_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ40220.1"
                     /translation="MQVYLTIGKKISGGFFILLVFTLLVAGASIYILKVTRDAYTRLI
                     EVNQQLVLEATQLRVAVLKKSEGYRGFLLYGQDSTLDVWLDGAREFRSIAEQMDLLLA
                     TPQEHQMVEEIVDLEKKLTEMQQQVIDLRRKGTSQDIVDEATKVRPFRIELFARVDQF
                     IQFQDRARSQVRDQVSTQITMVSIAMYLISAVALISAIFISWGLTRSITRQLRESIFQ
                     LSASSNEIVATTAQVASSAVETASAINQTTATVEEVKQTAQLATQKARNVSESAQKAT
                     QVSQKGKTAVAETLTGMERIKFQMESIAAHIVKLSEQNQLISEIIASVSDLAEQSNLL
                     AVNAAIEAAKAGDSGKGFGVVAQEVRSLAEQSKQATVQIRSILNQIQKAMTQAVMATE
                     QGAKVVETGVKLSLDAAETIRLLSESMSESSQAATQIVAS"
     gene            complement(1470..2090)
                     /locus_tag="PKZ53_07015"
     CDS             complement(1470..2090)
                     /locus_tag="PKZ53_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ40221.1"
                     /translation="MTEDRRGATTDWDDIYHRLNTTQAAIEQTQTPSGEISRQILRNR
                     ARQLALEPPEPASDVLSVAVIEFCLASETYGIELTSVREIIPLKELTPVPCTPPFVLG
                     LMNIRGQLLSVISLKKFFQLPEKGLTDLNKVIVVSVGGFEIGILADAVLTVKTILLSE
                     IQPAIHTFTGIHAQYLKGITREQTILLNLQELVTDQKLIISEQVDG"
     gene            complement(2108..>3583)
                     /locus_tag="PKZ53_07020"
     CDS             complement(2108..>3583)
                     /locus_tag="PKZ53_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNJ40222.1"
                     /translation="SESTLSQFCDLVSTHLGLHFSRERQGDLEYRLNQIASAFDFAHP
                     EACVNWLLTAPLTQKQIDVLAHHLTVGETYFFRDRHSCEALRLEILPALIQNWDQTGI
                     SPCIWSAGCCTGEEPYSIAILLDQLLPKSSRPAVRILATDVNPVFLQKAKTGVYSDWS
                     FRETPDWIKEKYFKRKGPAGHFELVPQIRKQVTFAYSNLANPCSFPELAANSVNLILC
                     RNVLMYFDAQSAHQIVQFFDRVLASDGWLLVSPSELSQVLFAPFQLTTVAGALCYTKH
                     LLPARPWFEQPELIPVTEEVTIQPELTLEGLVPTQDAPVLEPEIHPSPISPESRYAQA
                     LELYEARDYEPAMTTLQALLAVEPTNTLAMTLCGRICANQGLYDQAFEWCQVAIATDK
                     LTTAAYYLLATIYQEQNQIEAAVTALKQALYIEPNFIIAHFTLGVLALGQGWQRDSEK
                     QLRIALLLLATSDPQATVPHSEGMTAGQLTATIQSLLGQFR"
BASE COUNT          864 a          899 c          926 g          894 t
ORIGIN      
        1 ggaagcaaca atctgcgttg ccgcctgaga tgactcgctc atactttcgc tcagtaaccg
       61 aatggtttcg gcggcatcca gtgataactt cacaccggtt tccaccactt tggcaccctg
      121 ttcggtcgcc atcaccgcct gggtcatggc tttttgaatt tgattgagga tagatcgaat
      181 ttgcaccgtg gcctgttttg attgttcagc cagacttctt acttcttgag ccaccacgcc
      241 aaaccctttg ccagaatctc cggccttggc cgcttcaatc gcagcattga ccgccagcag
      301 gttagattgt tcggccaggt cgctcaccga tgcaataatt tcgctgatta actggttttg
      361 ctcgctcaat ttcacgatat gcgccgcaat agactccatc tgaaacttga tccgctccat
      421 cccggtcaga gtttcggcta ctgcggtttt ccctttttgg gaaacctggg tcgctttttg
      481 ggcgctttca gacacatttc tggctttttg agtggctaac tgggccgttt gtttgacttc
      541 ctcgacggtt gcggtggttt ggttgatcgc cgaggccgtc tcaacggcgc tggatgccac
      601 ctgggcagtg gtggccacaa tttcatttga agaagccgaa agttgaaaaa tactttcccg
      661 aagctgacgg gtgattgatc gggttagccc ccaggaaatg aagatagctg agatcaaagc
      721 cacggctgaa atcaggtaca tggcgattga aaccattgtg atttgagttg aaacctggtc
      781 gcgtacctgg ctgcgggccc gatcctgaaa ctggataaat tgatctaccc tggcaaagag
      841 ttcgattcga aacggacgga ctttggtcgc ttcgtctacg atgtcttgag aagtgccttt
      901 acggcgtaaa tcaatgactt gctgctgcat ctcagtcagc tttttctcaa ggtcaacgat
      961 ttcctcaacc atctgatgtt cctggggcgt ggcaagcaag agatccattt gttcggcaat
     1021 agaccgaaat tcccgtgcgc catccagcca cacatccagc gtagaatcct gtccatacag
     1081 caaaaagccg cgatagcctt cgcttttctt cagaacggca acccggagct gggtcgcctc
     1141 aagcaccagt tgctggttga cctcaatcaa ccgggtgtag gcatcacgcg ttactttcag
     1201 gatatagata ctggccccag caaccagcag ggtgaatacc aggaggatga aaaagcctcc
     1261 ggaaattttt ttcccaatcg tcaggtaaac ttgcatggta ggttcccttc ggtttgttca
     1321 gataaagaaa gccggttttt ttgaatgtct ggttgctggt atcaatcggg ggatttccaa
     1381 ccagttgccg gcaggatgcc ggcgttccca ggcaggaatc ctgttcggca gagttgtaga
     1441 atcggctgga gcatccccga aaacttcatt tatccgtcca cctgctctga aataataagc
     1501 ttttggtcag ttaccagctc ctggaggttc agtaaaattg tttgttcgcg ggtgattccc
     1561 ttgagatatt gggcgtggat cccggtaaaa gtatggattg ccggctggat ttcagacagt
     1621 aaaatggttt tgaccgtcag gactgcatca gccagaattc cgatttcaaa cccaccgact
     1681 gacacaacaa tgactttgtt cagatcggtc aacccttttt caggaagctg aaagaatttc
     1741 ttgagactga tgacggaaag aagctgcccc cgaatgttca tcaggccaag cacaaaaggt
     1801 ggggtgcagg gcacgggtgt cagctctttt aaggggatga tttcccgaac tgaggtcagt
     1861 tcgatgccat acgtttcaga cgccaggcag aactcaatca ccgcaacact caaaacatct
     1921 gatgcaggtt cgggaggctc aagcgccagt tggcgggccc ggttgcgcag aatttgccgg
     1981 cttatctccc cagatggggt ttgtgtctgc tcaattgcag cctgagttgt attcaaacga
     2041 tgatagatat catcccaatc tgtcgtggca cctcgccgat cttcagtcat acccaaatac
     2101 ccctctctta ccggaattga cccaggagtg attgaattgt ggctgtcaat tgtccagccg
     2161 tcatgccttc ggaatgcggc acggttgcct gtgggtcaga tgtcgctaac agcaaaagtg
     2221 caatccgcaa ctgtttctct gagtcccgtt gccagccctg accaagcgcc agaaccccca
     2281 gtgtaaaatg agcgatgata aaatttggct caatatagag tgcctgtttc aaggcggtga
     2341 cagcagcctc aatctggttc tgttcctgat agatggtggc cagcaagtaa taagcggcgg
     2401 tggtaagctt atcggtagca atggccacct ggcaccactc aaatgcctgg tcataaagac
     2461 cttgattggc acagatgcgc ccacacaacg tcattgccag agtattggtt ggctccacag
     2521 ccaggagcgc ctgcaatgtt gtcatggctg gctcatagtc gcgtgcttca tacaactcaa
     2581 gggcctgagc ataccgagat tctggcgaaa tcggtgacgg gtggatttcc ggttccagga
     2641 ctggtgcatc ttgagttggg accagcccct ccagggtcaa ctccggttga atcgttacct
     2701 cttcggtgac cgggatcaat tccggttgct caaaccaggg tctggccggc agcaggtgtt
     2761 tggtataaca cagtgctcca gcgacagttg tgagctgaaa cggggcaaat aacacctggg
     2821 acagctcgct ggggctcacc aggagccatc catcactggc cagtacccga tcaaaaaact
     2881 ggacgatctg gtgggctgac tgagcgtcaa aatacatcaa cacattgcga cacaaaatca
     2941 ggttgactga attggcagcc agctcaggaa atgaacaggg gttggccagg tttgaataag
     3001 caaacgtaac ctgttttctg atctgtggca caagttcaaa gtgcccggca ggcccttttc
     3061 gtttgaaata tttctccttt atccaatcgg gtgtctcacg aaaagaccag tctgaataaa
     3121 caccggtctt cgccttttga agaaaaaccg ggtttacatc ggtggcgaga atgcgaactg
     3181 cgggcctgga tgatttgggg agcagttgat ccagcaaaat cgcaattgaa tagggttctt
     3241 ctccggtaca acacccggct gaccagatac agggagaaat cccggtttgg tcccaattct
     3301 ggatcaaggc gggaaggatt tccaaccgga gtgcctcaca actgtgccgg tcgcgaaaga
     3361 aataggtttc cccgacggtc aaatggtggg ctaaaacatc gatttgcttt tgggtcagag
     3421 gtgctgtcaa caaccagttc acacaggctt ccggatgagc aaaatcaaaa gctgaagcaa
     3481 tctgattcaa ccggtattcg agatcgccct gacgctccct tgaaaaatgc agccccaggt
     3541 gagtcgaaac cagatcacag aattgggaaa gggttgattc tga
//
LOCUS       DAOQDO010000354         3581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19182_length_3581_cov_3.568633, whole genome shotgun sequence.
ACCESSION   DAOQDO010000354 DAOQDO010000000
VERSION     DAOQDO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19182_length_3581_cov_3.568633"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1066
                     /locus_tag="PKZ53_07025"
     CDS             230..1066
                     /locus_tag="PKZ53_07025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ40223.1"
                     /translation="MRKFRASLQISDTPNERCFGQPTRGKTAPNRLRQTDVFLAIAFP
                     GIIQTRNCRYVDLGYGESPITTLETWERLQKINPSIQVLGVEIDPNRVTDARPFTQEN
                     LEFRLGGFNLPVAPDERVNVIRVFNVLRQYEEADVEIALQTLGQSLAPGGLILEGTCD
                     PTGRFMAFQLFRKRNAGDIDHIGLVFAPRLRSEFYPRQLQSILPKSLIHHAEPGGAVD
                     QFFSRWHICWQAARAQFPGQAQKQIFAQAALRLKEIYGYHLDQRLTLLKRGFLVLSQK
                     HF"
     gene            1218..1784
                     /locus_tag="PKZ53_07030"
     CDS             1218..1784
                     /locus_tag="PKZ53_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNJ40224.1"
                     /translation="MKKVVIFSILVACVLIVCGLRSKAQTPVTTEQPIREALISAIDD
                     ERQAQAYYAAVLAKFGQVRPYTQLVKAEARHEEILVRYCEQNGIEVPANQWATPTMPL
                     PATLEEVRQQSLTREVKNVEMYDEFLKTIKTPALVTIFENLRSASLDHHIPALKGERM
                     GKGRGTGRNAQTCPLSGDQNCCAARKCK"
     gene            1939..2556
                     /locus_tag="PKZ53_07035"
     CDS             1939..2556
                     /locus_tag="PKZ53_07035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ40225.1"
                     /translation="METKPHWEKIYHTKAPDQVSWYQICPELSLNLIQQTGVNSSASI
                     IDVGGGASTLVDFLLQNGFANLTVLDLSAAALETSKRRLGDQAHQVTWIEADITQVHL
                     PEQGYDVWHDRAVFHFLTQVEDRHRYVEQVKQSVKPDGFVIVATFGPEGPLRCSGLEI
                     VRYSPDALHSEFGADFQLLESRQETHQTPFGSEQQFIYCFCRRTR"
     gene            2701..3396
                     /locus_tag="PKZ53_07040"
     CDS             2701..3396
                     /locus_tag="PKZ53_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009704132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNJ40226.1"
                     /translation="MKTIGLLGGMSWESTALYYKLINQGVATQLGGLHSAKILLYSVD
                     FAEIEALQREGNWEVAGQLLAQAAQKLATGGADFIVLCTNTMHKVASWIETASNLPLL
                     HIADATAERLLADGIHRVGLLATRYTMEQDFYRGRLADRYGLEVLVPAQPDRDIIHRV
                     IYDELCHGIIQPKSKAEYCRIIQTLIDQGAQGIIFGCTEIGMLVTASDVATSVYDTTQ
                     IHAEQAVYLALKE"
BASE COUNT          923 a          887 c          884 g          887 t
ORIGIN      
        1 tttttggtta aaacttttaa ccaaaaggtt aaagtgaggg ctgaagacat cgggcctggg
       61 gctgaaaata ccagggctca gggctcaggg cttggggctg aaaataccag ggctcagggc
      121 ttggggctga agaaaatggg ctgaaaaaaa cgggtttaat accccaagcc caatgtcttc
      181 agcccgatgt tttcagcccg atgccttcag cccaatttcc tcacagccta tgcgtaaatt
      241 ccgagcgtcc cttcaaattt cagacacacc caatgagcgg tgcttcggcc agccgacacg
      301 gggaaaaacc gcgcccaatc ggctgcggca gacagacgtc ttcctggcga ttgcttttcc
      361 tggaatcatc cagaccagga actgccgcta tgttgatctt ggctatggtg aatcaccaat
      421 tacgactctt gaaacctggg aacgcctgca aaaaatcaac ccttcgattc aggttctcgg
      481 ggttgaaatt gaccccaatc gggtgactga tgcccggcct ttcacccagg aaaatctgga
      541 gtttcgtctc ggaggattta acctgccggt ggcaccagat gaacgggtca atgtgatccg
      601 ggtttttaat gtgctccggc aatatgaaga ggctgacgtt gagattgcac tccagacact
      661 tggccagtca ctggcgccag gcgggctcat ccttgaagga acctgtgacc caactggacg
      721 ctttatggca tttcaactct tccggaaaag gaacgctggt gacattgacc acatcggcct
      781 ggtgtttgcc ccgcggctac gaagtgaatt ttatccgcgc cagttgcaat ccattctgcc
      841 caaaagcctg attcatcatg ctgagcccgg aggtgccgtt gaccaatttt tttcacgctg
      901 gcatatctgc tggcaggcgg cacgcgccca atttccaggg caggctcaaa aacagatttt
      961 tgcccaggcg gcactgagat taaaagaaat ttacggctat caccttgatc aacggctcac
     1021 gctgctcaaa cgcgggtttc tggtgctttc ccaaaaacac ttttaaccac gcaatacacc
     1081 aaaaaaatca aattccaaaa ctcccggaag caaccaaatt aaccgtgcgt tcaggtacga
     1141 tgtgctaggg tgagaggtac cgggagatgg tctctcgggt atacgtacct gcctcttagc
     1201 gggtactgga ggtcaatatg aaaaaagttg ttattttctc aattcttgtc gcctgtgtat
     1261 tgattgtttg tggactcaga tctaaagccc aaacacctgt caccaccgag cagccgatcc
     1321 gtgaagcgct tatttcagca attgacgatg agcgccaggc ccaggcgtat tatgcggctg
     1381 tacttgcgaa atttggtcag gttcgaccct acacccagct tgtaaaagct gaagctcgtc
     1441 acgaagaaat tctggtccgg tactgtgaac aaaacggcat tgaagttccg gccaaccagt
     1501 gggccacgcc caccatgcca cttccagcca cgcttgaaga agttcgtcag caaagtctga
     1561 cccgcgaagt caaaaatgtc gaaatgtatg acgaatttct gaaaaccatc aaaacaccag
     1621 cactggtcac gattttcgag aatttgcgaa gtgcatcgct tgatcaccat attccagccc
     1681 tgaaaggcga acgcatgggc aaaggccgtg ggactggccg aaacgctcag acctgtccac
     1741 tctcaggaga tcagaattgc tgtgctgcca gaaagtgcaa atgagaaagc gctacgctat
     1801 tttctggttg aaatttgagt gaagcaacga aaaatggcaa atccgaaagg gggtttgcca
     1861 tttttgtttt ggtgaatgag cggaggtata atagtatcaa ttatcagact catttaactt
     1921 tcaaggaagg aacccgccat ggaaacgaaa cctcactggg aaaaaattta tcacaccaaa
     1981 gccccagatc aggtgagctg gtatcaaata tgccccgaac tctcgctcaa cctgattcag
     2041 caaactgggg tcaactccag tgccagcatc attgatgtcg gtggtggggc ttcgactctg
     2101 gttgatttcc tgctccaaaa cggatttgcc aacctgacgg tccttgatct ttcagccgct
     2161 gcccttgaga cctcaaaacg ccgacttgga gaccaggccc atcaggtcac ctggatcgaa
     2221 gccgatatca cccaggttca tcttcccgaa cagggctatg atgtctggca cgaccgggct
     2281 gtgtttcatt tcctgaccca ggtcgaagac cggcatcggt atgttgaaca ggtcaaacag
     2341 agtgtcaaac ccgacgggtt tgtcattgtg gctactttcg gcccggaagg cccgttacgt
     2401 tgcagtgggc ttgagattgt tcgctatagc ccggatgctc tccacagcga atttggcgct
     2461 gattttcaac tgctggaaag ccgccaggaa acccaccaaa caccgtttgg atccgaacag
     2521 cagttcattt attgcttttg ccggcgaact cgataaagaa tattttggat accagaaatt
     2581 tttctgcctc ttcttcggtg tgttccgtgt attccgtggt ttcttttttc ttttcggttt
     2641 ttggcttttt tcgtgtattt tgtggtaaat ctctgagatc tttcagccag gtttttgctt
     2701 atgaaaacaa ttggtttact cggtgggatg agctgggaat caaccgcgct gtactacaaa
     2761 ttgatcaatc agggcgtggc gacacaattg ggcggcctgc attcggccaa aattctgctc
     2821 tatagcgttg attttgcgga aattgaagca ctccaacgcg aaggaaactg ggaggtcgcc
     2881 ggtcaactgc tggctcaggc agcccagaag ttagcaactg gtggcgccga ttttattgtg
     2941 ttgtgtacca ataccatgca taaagtcgcg agctggattg aaacagcatc caacctgcca
     3001 ctactccaca ttgccgatgc cactgccgaa cgattgctgg cggatggaat ccaccgggtg
     3061 gggttacttg caacgcggta cacaatggaa caggattttt atcgcgggcg actggccgac
     3121 cgatatggcc ttgaggtcct ggtcccagca caacccgacc gtgatattat tcaccgtgtg
     3181 atttatgacg agttatgtca cggaatcatc cagcccaaat caaaagctga atattgtcgc
     3241 attatccaga ctttgattga tcagggggcg caagggatta tctttggctg cactgaaatt
     3301 ggaatgctgg tgaccgcttc ggatgtggca accagcgtct atgacacaac ccaaattcac
     3361 gcggaacagg ctgtctacct cgcattgaaa gaatgaagaa tgaagaattg agaatgaaga
     3421 atgaagaatg aagaatgaag aattgagaat gaagaatgaa gaatgaagaa tgtaagctcc
     3481 tggtttcatc cctcatcctt catccctcat cccttcgatt gcccttagcc cttgcattgt
     3541 tgtcatccgg aaaaaaacct tgaccaatcg ttctaagtta a
//
LOCUS       DAOQDO010000355         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19216_length_3577_cov_3.762635, whole genome shotgun sequence.
ACCESSION   DAOQDO010000355 DAOQDO010000000
VERSION     DAOQDO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19216_length_3577_cov_3.762635"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="PKZ53_07045"
     CDS             complement(<1..1783)
                     /locus_tag="PKZ53_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNJ40227.1"
                     /translation="MSKIRIYDLAKELKLDNKKIMDEVRRLGYDVRVASNSVPDDIAE
                     KVRNLYFPKRATPAAPVKLMKGGHVVSEAVQPPPSVTPPRPVTTMPKLIKGVRRVESP
                     TLSTPSHEPEPPSTEIQPLRVVPLAPPPQVTPPVPVPRTLPTVHSIQPPPPVPVQPEP
                     PVVVPPPPISASPVQVEAPAKAPEPEVTTTRVIRIEPPAAPPVTPPVLPSVPSQEMTP
                     QPAPVSKTVPAPTVREISVPKPPPPPVEPKVAAPPPQHTPSVFQIRPITPPPVPPMAK
                     PAPPPPAAHSARPAPPTQRPTEAGTHTDDRRFPPRGQAPARRPQGEGDTRRGGEAPRP
                     GQPREASSEATPAPAPTRTTYIPPKDQKGRNDKPGARRPGGGGKEARTDSTNKREQHE
                     RDLRLRPTPTKLITPAPRPTFTELKPIRVPEGTTVKELAEKLEVKPKDVVALILTKGL
                     MVTINQAMTEEMMREVGREFGFEVSIGGFEEMVAEQEITEEPSTGDADEDRAPVVAVM
                     GHVDHGKTSLLDAIRNTRVAAGEAGGITQHIGAYSVEVPDPDDREKLRRIVFLDTPGH
                     EAFTLMRARGAKGADVAVIVVAADDGVM"
     gene            complement(1870..3333)
                     /gene="nusA"
                     /locus_tag="PKZ53_07050"
     CDS             complement(1870..3333)
                     /gene="nusA"
                     /locus_tag="PKZ53_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNJ40228.1"
                     /translation="MSSSPLAETIEQLCREKNIDHSIVISAVEDAVATAARKHFKTKE
                     DLHVIYNEETQQLELYALKQVTDEVHSPETEISLDEARAIFGEDVEVEVGDMLQFPRP
                     MEEMGRIAAQTAKQIIFQKVREAERNNIYNEYISRIGEMVNGFVKRFERGNMIVDLGK
                     IESILPRSQQSPVEPFNQGDRIRVVINNVSKEAKGPQVEVSRTSPELIKRLFETEVPE
                     IYDGTVVIKAAVREPGDRAKVAVYSNDPDVDPVGACVGMKGSRVQAVIRELRGEKIDI
                     IPWSEDPVVFAANALSPAKVSKVQIIDFQRQHLEVIVEDSQLSLAIGKKGQNVRLAAK
                     LVDWHIDIRSEGEMKRQVASQMEALLSAPTVPLAAVGEMNSSYLKKLAEAGIETVEQL
                     AECSVDDVAHILDVSLDEAQTLAEQAYQIMQIKSERMSEREASAASAGEADQPETWSD
                     SDGEPSDEASPEFDERLHQAAASTSEPEEEAESATQD"
BASE COUNT          659 a          917 c         1062 g          939 t
ORIGIN      
        1 gcatcacgcc gtcatcagcg gcaaccacaa tcacggccac gtcggcacct ttggcaccac
       61 gggcacgcat cagggtaaag gcttcgtggc ctggtgtatc aagaaacacg atccggcgca
      121 gtttttcacg gtcatccgga tcgggaactt caactgaata tgccccaatg tgctgggtga
      181 taccgcccgc ttcgccagcc gccacccggg tatttcgaat tgcatcgagc aggctggttt
      241 tcccgtggtc aacgtgaccc atgacggcca ccacgggtgc gcggtcttca tccgcatctc
      301 cggttgaggg ttcttccgtg atttcctgtt cggccaccat ttcttcaaag ccaccgattg
      361 aaacttcaaa gccaaattca cggccaactt cacgcatcat ctcttcggtc atggcctgat
      421 taattgtcac catcagaccc ttggtcagaa tcagcgccac cacgtctttt ggcttgactt
      481 caagtttttc agccagctct tttacggttg tgccttcagg aacgcggatg ggtttcagct
      541 cagtgaaggt tgggcgcgga gctggtgtaa tcagtttggt tggggttgga cgaagccgga
      601 gatcacgctc atgctgctcg cgcttattgg tgctatctgt tcgagcctct tttccaccgc
      661 caccaggtcg gcgtgcgccg ggcttgtcat tccgaccttt ctggtctttg ggtggaatgt
      721 aggttgtccg ggtcggagct ggtgcggggg tggcttccga agaagcttca cgcggttgac
      781 ctggtcgggg agcttcgccg cctcgccggg tgtcaccttc gccctgtggc cgtcgggctg
      841 gtgcctgacc acggggggga aatcgccggt catcggtgtg ggtgccagct tcggttggtc
      901 gctgtgttgg tggtgctggt cgggctgagt gggccgctgg tggtggtggg gccggtttgg
      961 ccatgggtgg caccggtggc ggcgtaattg ggcgaatttg aaacaccgat ggggtgtgct
     1021 gtggcggtgg ggccgcgact ttcggttcaa ctggcggcgg cggcggttta ggaacagaga
     1081 tttcgcgtac ggttggtgct ggaacagttt ttgaaacagg agctggttga ggggtcattt
     1141 cttgtgatgg aactgatggg agaactggtg gcgtcaccgg cggcgcggca ggtggttcaa
     1201 ttcgaatgac gcgcgtggtc gtgacttcgg gctcaggtgc tttggcgggt gcctcaacct
     1261 gaacgggact ggcactgatg ggtggaggtg gaacaacgac tggtggttca ggttgaacgg
     1321 gaactggcgg cggcggctgg atcgagtgaa ccgtcggcag tgtccgtgga actggaaccg
     1381 ggggcgttac ctgtggtggc ggggcaagtg gcaccactcg gagcggctga atctcggttg
     1441 atggaggttc tggttcgtgg ctgggtgttg acagcgtagg ggattcaacc cgtcttacgc
     1501 ctttgatcaa cttcggcata gtggtgactg gccggggagg tgtgacgctt ggcggtggct
     1561 gcaccgcttc gctgaccacg tgcccaccct tcatcaattt aaccggagcc gcgggagtcg
     1621 cccgtttggg aaagtagaga ttgcgtactt tctcggcaat atcgtccggg acggaatttg
     1681 acgctacacg aacgtcatat ccgaggcgac gcacctcatc catgatcttt ttgttgtcca
     1741 gcttcaactc tttggccaga tcgtaaattc tgatcttcga catgcacact ccgttttgat
     1801 tgcagtaatt gcgatagcgc caggctggtc agtggccgac tgggcagcct ttggccaatg
     1861 tctggttgat tagtcctggg tggccgattc ggcttcctcc tctggttcag aagtcgaggc
     1921 ggcggcttgg tggagccttt cgtcaaattc cggggaagct tcatcggagg gctccccgtc
     1981 actgtcggac caggtttcgg gttgatcggc ttcgccagcg ctggccgctg aagcctcccg
     2041 ctcgctcatc cgctctgatt taatctgcat aatttgatag gcctgttcgg ccagcgtctg
     2101 ggcttcgtcg agactgacat ccaggatatg agccacgtca tcaacactgc actccgccag
     2161 ttgttcaacc gtttcaatac cagcttccgc cagctttttg aggtaggagg aattcatttc
     2221 cccaacggcg gccagcggta cggttggcgc cgacaacaag gcttccattt gactggcgac
     2281 ctgtcgcttc atctcacctt cgctgcggat atcaatatgc cagtcaacca gtttggctgc
     2341 caggcgaaca ttttgtcctt ttttgccaat cgccaggcta agttgtgaat cttcaacgat
     2401 gacttcgagg tgctggcgtt gaaaatcaat gatctggact ttgctgactt tggctggaga
     2461 taacgcgttg gcggcaaaca ccaccgggtc ttccgaccag gggataatat caattttttc
     2521 gccccggagt tcacgaatca cagcctgaac acgtgatcct ttcatcccaa cgcaggcgcc
     2581 aaccgggtca acatccgggt cgtttgagta cacagcgact ttggcccgat cacctggttc
     2641 gcggacggcg gctttgatca ccacggttcc atcataaatt tcggggacct cggtttcaaa
     2701 gagtcgcttg atgagttccg ggctggtgcg tgaaacttcg acctgggggc ccttggcttc
     2761 tttgctgaca ttattgatga cgacacggat gcggtcacct tgattgaacg gttcgacggg
     2821 tgattgttgc gagcgaggca aaatagattc aatttttcca aggtcaacaa tcatgttacc
     2881 gcgttcaaat cgtttgacaa agccattcac catttcgccg atgcggctga tgtattcatt
     2941 gtaaatattg ttgcgctcag cttcgcggac tttctggaaa ataatctgtt tggccgtttg
     3001 agcagcaatg cgtcccattt cttccatcgg gcgcggaaat tgcagcatgt cgccaacttc
     3061 gacctcgaca tcttcgccaa aaattgcccg tgcttcatcg agtgagattt cagtttccgg
     3121 cgaatggact tcgtcggtca cctgcttgag cgcatagagt tcaagctgtt gcgtctcttc
     3181 gttatagatc acgtgcagat cttctttggt tttgaaatgt ttacgcgcgg cagtagccac
     3241 ggcgtcttcg acggcgctga tgacgattga atggtcaata tttttttcgc ggcacaactg
     3301 ttcaattgtt tccgctaatg gactgctgct catagactct ccgtgtattc gaaggaatat
     3361 gcggtatgtg tcggcagaag aggtgcgggg tgatccgtat ggtaaacggt ttccacggcc
     3421 tacgacggct tacgttgctg agatttttct ttagcaaccc ggaaaaggtc tggcaaattg
     3481 acttccaggt gcgctttttc aatccagtaa aacgggattt ccaggtcttt tcggttggtt
     3541 ttgcggttcc ctttgatcga gatcatgacc atcggct
//
LOCUS       DAOQDO010000356         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19261_length_3573_cov_2.742752, whole genome shotgun sequence.
ACCESSION   DAOQDO010000356 DAOQDO010000000
VERSION     DAOQDO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19261_length_3573_cov_2.742752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1881
                     /locus_tag="PKZ53_07055"
     CDS             118..1881
                     /locus_tag="PKZ53_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNJ40229.1"
                     /translation="MENFVLSGLVTELSALLTQAGHLRLGKLFALNLNGIALDVRLRD
                     NRILCLQPSPSDGRLYLTALHPRDLEARTTGEPPLLHRLRKDLGGATLSSVFKPLEER
                     SVTLTFSGFDVTGEPCTTRLRLDLMGRGSNLYWLAEDETILDVLRPTDQIIIGTKYYL
                     PPQADTFGWDEITPEHLEGLSGEALETWLRSNLRGCGRILAREIGFRTRSQSAWEAFL
                     SVRNQLTAKPSGFFYSRQPFDQLQVGAFDPHRDVCVAPFPLESLGSWSVREFPSLHEA
                     VDTWFQLIDAGTLLAARRQPWLTAANAHLKRTQSALKKLTAEPWDETEIENFRKFGEL
                     IFANIKTGERTPTGLIVVDYYDPDQRRIEIPVDEDEELSIAAQKYFRRYQKLQRALAA
                     RAERLKTLQAQLESAQTSVELLREAHTEAEIDDVVATIRPTIPLELRPKSQTVAKPGS
                     PNQTRAKQQNSLWSGLRHYRGPNDFDIYVGRTSQDNDRLTFKLARPNDIWLHSADYPG
                     AHVVIRNLSRQPVPHHVVVAAAELAAFFSQAKGLDKVNVHYTERKFLARPKQGAPGMV
                     LVLRSKTLLVPPREVLERVED"
     gene            2014..2787
                     /locus_tag="PKZ53_07060"
     CDS             2014..2787
                     /locus_tag="PKZ53_07060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ40230.1"
                     /translation="MPAMDYSRVAHYYDSYVQTSLDVNFFLQESKNAIRVLELMSGTG
                     RLSLPLIQAGVNLTCVDSSPEMLAILRQKLMQQNLQANVLEMDVCQMSLAGEFDLIVI
                     PFHSFAEILDPPDQRRALEEIFRLLSPSGRFICTLHNPPVRLKSVDGKVTFLGKHTLP
                     DNTGTLLLSTVQQYEASTHRVKGAQFYELYDTSGVMQSKWFVDLEFYLHQKNDFSKLI
                     QSIGFSTLNLYGDYSHSAFVENSSPFMLWVLGKALQPAK"
     gene            2979..>3573
                     /locus_tag="PKZ53_07065"
     CDS             2979..>3573
                     /locus_tag="PKZ53_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ40231.1"
                     /translation="MQKKDVLNAWGRILQGHAPSLSIEITRQCPLRCPGCYAYEPEHL
                     HQLGPLRDLADYKGKELVDNILKLVDRHRPLHLSIVGGEPLVRHRELNDLLPKLSERG
                     VVVQLVTSAVREIPKAWNTIKQLHIVVSIDGLQPDHDVRRAPATYERILKNIEDQTIT
                     VHCTITNQMAGKYNYFDDFLSFWSARPEVRKIWFSLFT"
BASE COUNT          838 a          989 c          886 g          860 t
ORIGIN      
        1 atctgtttat cggctgtgct gtttttgatc ttccattccc tcttcgcata gacacccaaa
       61 actaattttc catacaatgg cagtttttca cgtttcctct gaaagcactg tgattgtatg
      121 gaaaattttg ttttatccgg cctggttact gaattgtcag ccctcctcac tcaggcaggc
      181 catctccggc ttggtaaact ctttgcgctc aacctgaacg ggatcgccct cgatgtgcga
      241 ctgcgtgaca atcggatttt gtgcctccag cccagccctt ctgatggccg gttgtatcta
      301 accgcgctcc atccccgcga cctcgaagcc cgtaccaccg gcgaaccacc cctcctgcac
      361 cggctgcgaa aagatctggg aggtgcaacg ctgagttctg tgttcaaacc gcttgaagaa
      421 cgctccgtta cgctgacgtt ttccgggttt gatgttacgg gtgaaccctg taccactcgc
      481 ctgcggcttg atttgatggg acgtgggagc aatttgtact ggctggctga agacgaaacc
      541 attctggatg tccttcgtcc aactgatcaa atcatcatcg ggacgaaata ttatctgcca
      601 ccgcaggctg acacgttcgg atgggatgaa atcaccccgg agcatctgga agggttgtca
      661 ggtgaagcgc ttgaaacgtg gcttcggtca aacctgcgtg gctgtggccg gattctggct
      721 cgtgaaattg gcttccgaac cagaagccag tcggcctggg aagcttttct cagcgtcaga
      781 aatcaactga ccgccaaacc atccggcttt ttctacagtc gccagccctt tgatcagctt
      841 caggttgggg cgtttgatcc acatcgtgat gtgtgtgtcg ctccctttcc gctggaatcc
      901 ctgggaagct ggtcagtgcg ggagtttccg tcactccacg aagcggttga cacctggttt
      961 caattgattg atgcgggcac gctgctggct gcccgtcgcc agccgtggtt gaccgccgcc
     1021 aatgcccacc tcaaacgaac ccaatcggcg ctcaagaaac tgacggcgga accctgggat
     1081 gaaactgaaa tcgagaattt tcggaaattc ggtgaactga ttttcgccaa cattaaaact
     1141 ggagagcgaa ctccaaccgg gctgatcgtg gtggactact acgatccgga tcaacgccgg
     1201 attgaaattc cggtggacga agatgaagag ctttctatcg ccgcgcaaaa gtactttcga
     1261 cgctatcaga aattgcagcg ggcgctcgcg gcgcgtgccg aacgcctgaa aacactgcag
     1321 gcacaacttg aatcagccca aacttcggtt gaactgctcc gcgaagcgca taccgaagcc
     1381 gaaattgatg atgtcgtggc gacgattcgg cccacgattc cgcttgaact ccggcctaaa
     1441 tcgcaaacgg ttgccaaacc aggctcgcca aaccagaccc gagccaagca gcaaaactcg
     1501 ctctggtcag gtttgcggca ttatcgcggc ccaaatgact ttgacattta cgttgggcga
     1561 accagccagg acaacgaccg gttgaccttt aaactggcgc gaccaaacga catctggctg
     1621 cattcggcgg attatccagg cgcgcacgtt gtcattcgga atctgtcacg acaaccagtt
     1681 ccccatcacg tggtcgttgc ggcagccgaa ctggccgcct ttttttcaca ggcgaagggc
     1741 ctcgataaag tgaatgtcca ttacaccgag cgaaaattcc tggcgcgacc caaacagggc
     1801 gcgccaggaa tggtccttgt cctgcgaagc aaaaccctgc tcgttccccc acgtgaagtg
     1861 ctggaacggg tcgaggatta aagaagtgat ttctttggag tgcagggact tgtcaccgct
     1921 tttctacata gcgacgtgtc gctgtctgac aaagctgcaa caagttgcag cactccacat
     1981 acagattttg aactttttgg agatgtaacc cctatgccgg caatggatta ctcgcgtgtg
     2041 gcgcattact acgactcata tgtccagacc agtctggacg tcaatttttt ccttcaggaa
     2101 tcaaaaaacg ccattcgcgt gcttgaattg atgtcaggca ccgggcggct ttccctgccg
     2161 ctgatccagg ccggagtgaa cctgacctgt gttgatagct cgccggagat gctggcgatc
     2221 ctgcgccaga aactgatgca gcagaacctc caggccaatg tgcttgaaat ggatgtatgc
     2281 cagatgtcac ttgccggaga atttgacctg attgtcattc cgtttcactc gtttgccgaa
     2341 atcctggacc cacccgacca gcggcgcgca ctggaagaaa tcttccgcct gctttcccct
     2401 tccgggcggt ttatctgcac gcttcacaat cccccggtca gactcaagtc ggttgatggc
     2461 aaggtgacgt ttctgggtaa acataccctg cccgacaata ccggaacgct cttactctcc
     2521 acggttcagc aatatgaagc ctcaacacac cgggtgaaag gggctcagtt ttatgagttg
     2581 tatgacacca gtggcgtgat gcagtcaaag tggttcgtag accttgaatt ttacctgcac
     2641 caaaagaatg atttctcaaa acttattcaa agtattggat tttcgacact caacctctat
     2701 ggagattact cacattcggc ttttgttgaa aactcaagcc ccttcatgct ttgggtgtta
     2761 ggaaaagcac ttcagccagc caaatgaacg acgttctttc agaaaatcag atagttttgc
     2821 cggattgggg taaagcgcta tatctatgag agttaccccg cctgacgacg gcttcaactg
     2881 acaataaacg ctttcggttt atcctgtatc tacttgactt tcctggattt tacggcactc
     2941 tcttccccac acgactccaa cctgaaggga ttgcttgcat gcagaaaaag gatgttctga
     3001 acgcctgggg gcgcatttta caagggcacg cgccatcact ctcaatcgaa atcacacgtc
     3061 aatgcccgct tcggtgcccc ggttgctacg cctatgaacc agaacacctg caccagcttg
     3121 gtccgttgcg cgacctggct gattacaaag gaaaagagct ggttgataac attttaaagc
     3181 tcgttgaccg ccatcgtccg ctgcaccttt cgattgtggg cggcgagccg ctggtccgcc
     3241 atcgtgaact caatgacctg ctgccgaaac taagcgagcg aggcgtggtg gtgcaactgg
     3301 tgaccagcgc cgtccgtgaa atccccaaag cctggaatac catcaagcaa ttgcatatcg
     3361 tcgtgtcaat tgacggactg caaccagacc atgatgtgcg ccgcgcccca gccacctatg
     3421 aacggattct gaaaaacatc gaagaccaga caattaccgt gcattgcact atcaccaacc
     3481 agatggcggg caaatacaat tattttgatg attttctgtc attctggtca gcccgacctg
     3541 aagtgcgcaa aatctggttc agtctcttca cac
//
LOCUS       DAOQDO010000357         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19309_length_3568_cov_3.105892, whole genome shotgun sequence.
ACCESSION   DAOQDO010000357 DAOQDO010000000
VERSION     DAOQDO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19309_length_3568_cov_3.105892"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..890)
                     /locus_tag="PKZ53_07070"
     CDS             complement(138..890)
                     /locus_tag="PKZ53_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ40232.1"
                     /translation="MKETIIVDHLWKKFRRFSKFRPRTLKDLLIQGFRELKPTETFWA
                     LRDVSFTVGPGEMVGIIGKNGAGKSTLLRLVGGVGLPNEGKITVEKPVRGLLELGAGF
                     SPDLTGRENAYVAGVIDGLTRRQVSQRLDSIIDFAELGAYIDQPLRTYSTGMRLRLAF
                     AVAVHTEPRVLLIDEVLAVGDAAFRTKCIDRISRFRDDGCTILFVSHDEEQVRKFCDR
                     VLWLHYGEVITYGPTQEVLKNYLTQMNQNDAL"
     gene            complement(890..1669)
                     /locus_tag="PKZ53_07075"
     CDS             complement(890..1669)
                     /locus_tag="PKZ53_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40233.1"
                     /translation="MANIDLYRLEYLFDLIRELVSREIKIQYKRSYLGVAWTIALPLT
                     QLIVFSVVFKGVLKLDIPNYGAFVFCGLLVWNWFQLSTVQGAVAIIHKPELISRPGFP
                     TWVLPIVFVTINLIHFLVSLPFLVVLLLMKGGVLTWWVLLLPAVVAIQFVFLLGLSLI
                     VATTNVVLRDTQHLLSVMLPLLFYLTPVCYDVKLVPASLKWVYQLNPLTHLLEAYRSI
                     LIFGKPPELTGLLLLSLLSVIVVWYGNRIFHQKSHTFIEEL"
     gene            1930..>3568
                     /locus_tag="PKZ53_07080"
     CDS             1930..>3568
                     /locus_tag="PKZ53_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNJ40234.1"
                     /translation="MNRRKAALDYHSEGRKGKIEVVPTKPCLTQRDLSLAYTPGVAEP
                     CLEIARDPDTAYSYTAKGNLVAVITNGTAVLGLGNIGPLAGKPVMEGKAVLFKRFADI
                     DVFDLELATNDPEEIIRTVKILEPTFGGINLEDIKAPECFYIEKRLREELQIPVFHDD
                     QHGTAIISAAALINALELVGKKIHDIKMVFSGAGAAAMGCARLYIRLGVNPNHILMCD
                     SHGVIYKGREQGMNEHKLEFAVETEARTLSDAMQGADVFIGLSVKGMVTGDMLKLMGP
                     KPIIFAMANPDPEIGYDEAKEARPDCIMCTGRSDYPNQVNNVLGFPFIFRGALDVRAR
                     CMNEEMMIGAVNALAALAKEDVPDSVAKAYGLSHLSFGPEYIIPKPFDYRVLIWACSA
                     VAEAAMKSGVARISIDLDEYREQLISRLGKSHEVMRMMIAKARRDPKRIVFPESSHER
                     ILRAAQALVDDRIAKPILIGKESQIRPLAEKYHVDLKGVEFVDPDTFPKIEAYTDELF
                     RLRQRKGLNRQETSEWLKHPNVFGPMMVRMGDADALLC"
BASE COUNT          933 a          944 c          888 g          803 t
ORIGIN      
        1 ggttccgggt tccgcgcttc cgggttccgg gttccgcgct tccgggttcc gggttttcga
       61 atttatgtcc tttatgtctt ttatgtccct tttgtctttt tcctgacccc tgacccctga
      121 cccctgaacc ctatgtttta aagggcatca ttctgattca tctgggtcag atagttcttg
      181 agcacttcct gagtggggcc ataggtgatg acttcgccat aatgcagcca cagcacccgg
      241 tcacaaaatt tgcggacctg ttcttcatcg tgggatacaa acagaatggt gcagccgtca
      301 tcccgaaacc tggaaatgcg atcaatacat tttgttcgaa aggcggcgtc tccgactgcc
      361 agcacttcgt caatgagcag gacgcggggt tcggtgtgaa cggccacggc aaacgccagt
      421 cgaagtcgca tcccggtgct gtaggtgcgc aatggctggt cgatataggc acccagttcg
      481 gcaaaatcaa taatcgagtc caatcgttgt gaaacctgac ggcgtgtgag gccatcaatg
      541 accccggcca cataggcatt ttcgcgcccc gtcaaatcgg gtgaaaatcc ggcgcccagt
      601 tccagcaatc cacgcactgg tttttcaacc gtgatttttc cttcgttggg cagccccaca
      661 cctcccacca gtcggaggag ggttgatttt ccagccccgt ttttgccgat aatgccgacc
      721 atttcccctg ggccgacagt aaacgacaca tcacgtagcg cccaaaaagt ctcggttggt
      781 ttcagttcgc gaaacccctg aatcaacaga tctttgaggg tacgggggcg aaacttgcta
      841 aaccgacgaa actttttcca caaatggtca acgatgatgg tttctttcat cacaattcct
      901 cgataaatgt atgactcttt tgatgaaaga tccggtttcc ataccagacc acaatcaccg
      961 acagcaacga caacaggagc aggccagtca attctggcgg tttgccgaaa atcaaaatgc
     1021 tgcgatacgc ctcaagcagg tgagtcagag gattgagctg atagacccat ttcagcgatg
     1081 cggggactaa cttcacgtca taacaaaccg gagttaagta aaacaaaagc ggcaacatga
     1141 cgcttaataa atgctgcgta tcacgcaaca cgacgttcgt tgtcgcgaca atcaaactca
     1201 gtcccagcaa aaacacaaac tgaattgcca cgactgccgg gagcaagagc acccaccacg
     1261 tcagaactcc ccctttcatc agcaacagca cgaccagaaa gggcagcgat accaggaaat
     1321 gaatcaggtt gatggtgaca aagacaatcg gcagcaccca ggttggaaat ccaggccggg
     1381 aaatgagttc cggtttatgg ataattgcca cggccccttg aactgttgaa agctgaaacc
     1441 agttccagac cagcaagcca caaaagacaa aggctccgta attggggata tccagtttca
     1501 acacgccctt aaacacaact gaaaaaacaa tcagttgggt caacggaagg gcgatagtcc
     1561 acgccacacc aaggtatgag cgcttgtact ggattttaat ttcgcgtgaa accagctcgc
     1621 gaatcaggtc aaacagatat tccagccgat agagatcaat atttgccaca aaggttgtcg
     1681 tttcaaaaga aggtaaaaac actgcccggg gtgaactggg caaagtactg atttcacaac
     1741 acaacgtcaa tatggagtgc ggtagcttga tgccgctttc ggatactcca aagaaattat
     1801 ttgcagccag ggatcagttc aactactttt ccacactcat caccccaaat cttgctcctg
     1861 cttcacccca agaaaggaaa tctggtctca acctgcctcc actggccgaa ggctgaccag
     1921 ccagcgtcta tgaatcgtcg aaaagctgct cttgattacc atagcgaagg tcggaaagga
     1981 aaaatcgaag tcgtcccaac caaaccttgc ctgactcagc gtgatttatc gttggcctac
     2041 acgccaggcg tagccgaacc ctgcctggag attgcccgtg acccggatac ggcctacagc
     2101 tataccgcca aaggaaatct ggttgcggtg attaccaatg gaaccgcggt gcttggactt
     2161 ggcaacattg gcccgctggc tggaaaaccg gtgatggaag gaaaagccgt tttgtttaaa
     2221 cgctttgccg atattgatgt ttttgacctt gagctggcca ccaatgatcc ggaggagatt
     2281 atccgaacgg taaagatcct ggaaccgacg tttggcggca tcaaccttga agacatcaaa
     2341 gcgccagagt gtttttacat cgagaaacgg ctccgggagg aactccaaat cccggtcttt
     2401 cacgatgatc aacacggtac agcgattatt tcagccgcag ccctgatcaa tgccctcgaa
     2461 ctggttggaa aaaagattca cgacatcaaa atggtgtttt ccggcgctgg agccgcagcg
     2521 atggggtgtg cccggctcta tattcgcctc ggggtcaatc cgaaccacat tttaatgtgt
     2581 gacagccacg gtgtgatcta taaaggccgc gaacagggga tgaacgagca caaactggaa
     2641 tttgccgtcg aaaccgaagc tcgaaccctg tcagatgcca tgcagggtgc cgatgtcttc
     2701 attggacttt cagtcaaagg catggtcacg ggcgacatgc taaaactgat gggaccgaag
     2761 ccgatcattt ttgcaatggc caacccggat ccggaaattg gctatgacga agcgaaagaa
     2821 gcccgcccgg actgcattat gtgtaccgga cgatctgatt accccaatca ggtcaacaac
     2881 gtactgggct ttcccttcat tttccgtgga gcgcttgatg tgcgtgcccg gtgtatgaat
     2941 gaggaaatga tgattggtgc cgtcaatgcc ctggcggcgc tggccaaaga ggatgttccc
     3001 gattcagtgg ccaaagccta tggcctgtca cacctgtcgt ttgggccgga atacatcatt
     3061 cccaaaccgt ttgactaccg ggtcctcatc tgggcctgtt cggcagtggc tgaagccgcc
     3121 atgaaaagcg gtgtcgctcg catctcgatt gacctcgatg aataccgcga acagctcatc
     3181 agccggctcg gaaaatcaca cgaagtgatg cgcatgatga ttgccaaggc ccggcgcgat
     3241 ccaaagcgaa tcgtcttccc agaaagctcg cacgaacgca ttttgcgagc cgcccaggcc
     3301 ctggttgatg atcgaattgc caaacccatc ttgattggca aggaatcgca gattcgtccg
     3361 ctggccgaaa aataccacgt tgatttaaaa ggtgttgagt ttgttgaccc cgacacgttt
     3421 ccaaaaattg aagcctatac cgatgaactg tttcggctcc gtcaacgcaa aggcttgaac
     3481 cgccaggaaa cttcagaatg gttgaaacac cccaatgtat ttgggccaat gatggttcgc
     3541 atgggcgatg ccgatgcctt gctgtgcg
//
LOCUS       DAOQDO010000358         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19311_length_3568_cov_2.935383, whole genome shotgun sequence.
ACCESSION   DAOQDO010000358 DAOQDO010000000
VERSION     DAOQDO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19311_length_3568_cov_2.935383"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3397)
                     /locus_tag="PKZ53_07085"
     CDS             complement(<1..3397)
                     /locus_tag="PKZ53_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknK"
                     /protein_id="HNJ40235.1"
                     /translation="MFTLSGYHIQKKYFESGHSLIFRAERDSDQLPVVLKVLKNEYPT
                     LEELARFRQEYEILKGLESERIIRVYALESFQRTPVIVLEDFGGESLLGLLPGLNLTI
                     DAFLTLAIELAKALGEIQQHHIIHKDINPSNIIYNQSTGQLKLIDFGISTVLTRENPI
                     LRNPTVLEGTLAYISPEQTGRMNRAIDYRTDFYSLGITLYEILTGQLPFVSDDAMELV
                     HWHLAKQPLPPEELRPDTPVMVSRLILKLMAKTAEERYQSAHGLLLDLQRCQELVQSG
                     QPNVVFQPGQEDFSDRFQIPQRLYGRQAEIEQLLAAFDRVSQGHREMMLVVGYSGIGK
                     SALVQEIYKPITERRGYFVAGKFDQLQRNIPYTALIQACDSLIHQLLTEPEEKIASWR
                     KSLRLALGANGQVIAEVLPAIELIVGPQPPVEALPPFESQNRFHRVFRQFIKVFAQTE
                     HPLVMFVDDLQWADAASLKLIHWLVTEPRPSYLFVIGAYRDNEVHLTHPLIVALDEIL
                     RSDVALTQVRLSRLELPHVLQLVADTLSCLPSDARPLAELVLAKTQGNPFFMNEFLKS
                     LAADGLIWFDFPTGRWLWDVAQIQECNITDNVVELMAGKVQKLDPPTQELLKLAACVG
                     NHFDIHTLAIVSGKTPHTTIRTLRTAVSEGLVTPLGDSYKLLELDLPDRQQGLRAEYK
                     FLHDRIQQAVYSLISGTDRQNVHLLIGRQLLEQTPESRREQVIFDIVNHLNQATSRLN
                     RPADLQQLAELNLAAARKAKASAAFELMYTYLQHGLKLLPGNRWESVYHLTLDFYIEI
                     AEAAFLNTDFSSFDRYARTALEQTHSLLDVLKIYEVQIQACMAQNQQLKAIETAVGIL
                     NQLGVSLPEKPTARHFRRELAHTRALLAGKSIQDLLDWPLMTDPKKQAALRILAGITS
                     AAYQALPALFPLVTFQQVNLSVDYGNAPLSAYAYGLYGVVLCGLAQDIETGYQFGQLG
                     LRLLEKFGSKELAAKTVFSVNVTVRNWKEHLKETISSFQEAYQNGLETGDLEYAALCA
                     FGHCYHAYHIGAELTALEQVMAAYREAPGHLLHLIQLYRQVVLNLIGKNNHPCQLIGV
                     SYDETVSVAFHERANNRTALARYWMNKAMLGYWFDEFETAYPAI"
BASE COUNT          863 a          921 c          925 g          859 t
ORIGIN      
        1 gaattgccgg atatgccgtt tcaaactcgt caaaccagta tccgagcatg gctttgttca
       61 tccagtatcg ggccagagcg gtacggttgt tggcgcgctc gtgaaacgcc acggagactg
      121 tttcgtcata tgacacaccg atcaactggc agggatggtt gtttttgccg atcagattca
      181 ggaccacctg gcgatagagt tgaatcaaat gcagcaaatg gcctggcgct tcgcggtagg
      241 cagccataac ctgttccagg gctgttagtt cggcgccaat gtgataggca tggtaacaat
      301 ggccaaacgc acacagcgca gcatattcca gatcaccggt ttcaagtccg ttttggtagg
      361 cttcctgaaa agacgagatg gtttctttga ggtgttcttt ccagtttctg acggtgacat
      421 tgactgaaaa caccgtcttg gccgccagtt ccttcgagcc aaatttttcc agcagccgca
      481 accccaattg tccaaactga tacccggtct cgatgtcttg agcgagccca cagagcacca
      541 cgccgtacag cccataggca taggctgaaa gtggagcgtt cccatagtca accgataaat
      601 taacctgctg aaaggtcacc agcgggaaca acgccggcaa ggcctggtac gccgccgaag
      661 taatcccagc caaaatacgg agcgcggcct gtttttttgg atccgtcatc agtggccagt
      721 caagcaaatc ctggatggat ttaccagcca gcagcgcccg cgtatgggcc agttcgcggc
      781 gaaagtgcct ggcagtcggt ttttctggta agctcacccc aagctgattg aggatgccaa
      841 ctgccgtctc aattgccttg agttgctggt tttgggccat gcacgcctgg atctggactt
      901 cataaatttt cagcacatcc agcagcgaat gcgtctgctc aagtgcggtc cgggcatagc
      961 gatcaaaact ggaaaagtcc gtattgagaa aggcggcttc ggcaatttca atatagaagt
     1021 ccagcgtgag gtgatacacg ctttcccatc ggttgcccgg cagcagtttg agcccgtgct
     1081 gcaaataggt gtacatcaat tcaaatgctg ctgaagcctt ggcttttcga gccgcggcca
     1141 gattcagctc tgccagttgc tgcagatcag ccggacgatt caaccgtgac gtggcctgat
     1201 tgagatgatt gacaatgtca aaaatcacct gttcacgacg actctcaggg gtttgttcga
     1261 gcagttgccg cccgatcaac aaatgaacat tttgtctatc ggttcctgaa atcagtgaat
     1321 agacagcctg ctggatgcgg tcatgcagaa atttatattc ggcccttagc ccctgttgcc
     1381 gatccggaag gtcaagctcc agtaatttat agctatctcc gagcggtgtg accagacctt
     1441 cactgaccgc cgttcgcaag gtgcgaattg tagtgtgcgg tgttttgccg gaaacaatcg
     1501 ccagggtatg aatatcaaag tgattcccga cacaggcggc cagcttcaga agctcctggg
     1561 ttggcgggtc aagtttttgc actttacccg ccatcagttc aaccacattg tcagtgatgt
     1621 tgcattcttg aatctgtgcc acatcccata gccagcggcc agtcgggaag tcaaaccaga
     1681 tcaatccatc agccgccagc gacttgagaa attcattcat aaagaacgga tttccctggg
     1741 ttttcgccag taccagttcc gccagcggac gggcatcaga tggcagacaa ctcaatgtat
     1801 cggccacaag ctgcagaacg tgaggtaatt ccagccgcga gagccggacc tgagtcagtg
     1861 ccacatcgga tcgcagaatt tcatcgagcg ccacaatcaa cggatgcgtc aaatgcactt
     1921 cattgtcgcg atacgcacca atcacaaaca aataactggg cctgggttcg gtcaccagcc
     1981 agtggatcag tttgagtgag gcggcatccg cccattgtaa atcatccaca aacatcacca
     2041 gcgggtgctc agtctgggca aacaccttga taaactggcg aaacacccga tgaaagcggt
     2101 tttgcgattc aaacgggggc agtgcttcaa ccgggggttg tggtcccaca attaactcaa
     2161 tcgctggcaa gacttcggcg atgacctgcc cattggcgcc cagtgccagg cgaaggctct
     2221 ttcgccagga ggcaattttt tcttctggtt cggttaaaag ctggtgaata agcgaatcac
     2281 acgcctggat caaggcggtg tagggaatat tgcgctggag ttgatcaaat tttccggcga
     2341 caaaatagcc ccggcgctcg gtaattggtt tataaatttc ctggaccagg gctgatttcc
     2401 caatgcccga atacccgacc accagcatca tttcgcgatg cccctggctc acccgatcaa
     2461 aggccgccag taattgttcg atttccgcct gccggccata gagtctttgg ggaatctgaa
     2521 accggtctga gaaatcttcc tgtccaggtt gaaacacaac gttcggttgc cctgactgga
     2581 ccagttcctg acaccgttgc agatcaagca acaacccgtg ggcgctttga tagcgctctt
     2641 cagcggtttt tgccatcaac tttaaaatca ggcgtgagac catgaccgga gtatctggcc
     2701 gcaattcttc aggcggaagc ggttgtttgg ccaggtgcca gtgcacaagt tccatcgcat
     2761 catctgacac aaagggcagt tgcccggtga gtatctcgta taaagtaatt ccaagtgagt
     2821 aaaaatcagt gcggtaatca attgcccgat tcatccgccc ggtttgttcg ggtgagatat
     2881 aggccagagt tccttcgagc acagttgggt tgcgcaggat cggattttcg cgagtcagca
     2941 cggttgaaat cccgaaatca atcaatttca actgcccggt tgactggtta tagatgatat
     3001 ttgagggatt gatgtcttta tggataatgt ggtgctgctg gatctcgccg agcgcttttg
     3061 ccagttcaat ggccagcgtg agaaaggcgt caatggtcag attcaatcct ggtaagagac
     3121 ccagcaatga ctcgccgcca aagtcttcaa gcacaatcac cggggtgcgc tgaaacgatt
     3181 ccagggcgta gacccggata atccgctctg attccagacc tttcagaatc tcatactcct
     3241 gtcgaaaccg cgccagttct tccagggtcg gatactcgtt tttcaacact ttgagaacaa
     3301 ctggcaactg gtctgaatct cgctcagccc gaaaaatcag ggagtgcccg ctctcaaagt
     3361 attttttctg aatgtgatat ccggaaagcg taaacaagtg cggtctcgct ccttgtgctt
     3421 ggttctttgt gtttggcccc tggtgctttg tgcttcgaaa agagaagtgt tcctaagcac
     3481 caggtaccaa gtaccagcag tcactgcgat tgaaggggag ttaccaggcg atgtactcag
     3541 aaattcagat gtgcgcaagt gttggatc
//
LOCUS       DAOQDO010000359         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19341_length_3564_cov_3.330579, whole genome shotgun sequence.
ACCESSION   DAOQDO010000359 DAOQDO010000000
VERSION     DAOQDO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19341_length_3564_cov_3.330579"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..825)
                     /locus_tag="PKZ53_07090"
     CDS             complement(172..825)
                     /locus_tag="PKZ53_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ40236.1"
                     /translation="MSTEPELHIIIADDHPIFRRGLHMIIEADPRLKVVAEADDGAVA
                     LAEVKRLQPHVVVLDMDMPVLDGMGVAREILQQRWPVEIAFLTMHKEELIFNAALDLG
                     VKGYVVKDSAATEIVDCIKAIASGQSFISPVLSGLLLNRVAPGTASKAPGLSVLTPAE
                     RRVLRLIADSKLNTEIAAELFVSVRTVEHHRSNICSKLGLNGKHALLTFALTHKSEI"
     gene            complement(815..>3564)
                     /locus_tag="PKZ53_07095"
     CDS             complement(815..>3564)
                     /locus_tag="PKZ53_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40237.1"
                     /translation="GNSRGLTSNRFSSLFEDTDGTLWIGTIDGGLTRLRDGVFTTFTT
                     ADGLPHNWIHNIQRQSNGELLITTENGFAYWREGQFIADPERLNSSTMVFFTGPTGTD
                     WRWDAEGLHRLTGHRITFPIKFKNYGVKHLHPFEDQQGNLWVGTFNDGVYVITGDRFR
                     HFTSQDGLPARKLILPQCQDRAGDIWFSSEDLTGNGLSALLHFKNGRFSSYPTSMIIR
                     TIFEDREGTLWIGTNGQGLNRLTYQFITTYSTANGLVGNNVYPIYQDRAGSIWIGAFG
                     GLSRYKDGSFTNYTVREGLLSPNVQAFCEDNAGRLWMGGRPSFFQNGRFYMPPDGIPF
                     MDYMCFAIYQDRSGVLWFGTHRGLVKYEHGKTFFLTVEDGLPGSDVLAIHQDRQGVLW
                     FATGGGIARLENGKFTVFTVADGLTTNYMRCIYEDAEGVLWFGTYDGGLIRYQTGRFT
                     CYTTREGLHNNGVFQILEDNRGNFWMSCNQGIYRVSRRQLNDFAAGKIQAITSIAYNK
                     HDGMLNIECNGGRQPAGIKARDGKLWFPTQEGVAIVDPEAVPTNSLAPSVLIETCRLD
                     REVAAFQGGLRISPGQSNLEIQYTGLSFIKPEQMRFKYQLIGQDPDWVDAGTRRMANY
                     SYLPPGEYTFKVIAANSDGVWNLSGVELQIVVLPRFYQTWWFRGLIVLMLAGITGLVF
                     KVRLNQVERARRVQEAFSRRLIDSQEQERQRIAAELHDSLGQNLLVIKNYALMGLTTA
                     EDDNPMREHLVEISDAATLSIEEVRQIAHNLRPYQLERLGLTRTLQSMLQQIANASDI
                     GFTWEIDQIDGLLSKEGEIGLYRIVQEALNNILKHSKASDASVSIQLDGDELHVTITD
                     NGCGFTVEASGTAELQKRGFGLTGSAERVRMLGGKQTIRSAPGQGTTIFITITTNRHV
                     N"
BASE COUNT          859 a          991 c          884 g          830 t
ORIGIN      
        1 aagaagtcgg gctgaagaag tcgggctgaa gaagtcgggc tgaagacatc gggctgaaga
       61 taccgatgag tcagtaccac ccgcgtcagt gggtgggccg gctggcttct aaccccaccc
      121 gctgacgcag gtggtactga caggtccttt atgttgttgt cgttttcctg actaaatctc
      181 tgatttgtgt gtcagggcaa aggtcaggag cgcgtgtttg ccattgagtc ccaatttgga
      241 gcagatattt gaacgatggt gctcgacagt gcggacgctg acgaaaagct cagccgcaat
      301 ttcggtgttg agtttactgt cggcgatcaa acgcagaacg cgtcgctcgg ctggtgtcag
      361 aacgctgagt cccggagcct tactggctgt ccctggcgca acccgattca gtaacaaacc
      421 cgaaagtacc gggctgataa aactctgtcc gctcgcaatg gctttaatgc agtcaacaat
      481 ttcggtggcg gcactatctt tgacaacata gcctttgaca cccagatcaa gcgcggcgtt
      541 gaagatcaat tcttccttgt gcatggtcag aaaggcaatt tcgactggcc agcgctgctg
      601 caggatttcg cgtgccaccc ccatcccatc gagtacaggc atatccatat cgagcaccac
      661 aacatgcggt tgaagccgct tgacttcggc cagcgccacg gcaccgtcat cggcttcagc
      721 cacgactttt aaccgtggat cggcttcgat aatcatatgc agcccacgcc gaaagatggg
      781 gtgatcatcg gcaataatga tgtgcagttc aggttcagtt gacatggcgg tttgtggtga
      841 tggtaatgaa gatggtcgta ccctggccgg gcgccgaacg gatcgtttgt tttccaccca
      901 gcatcctcac gcgctcagca ctacccgtca agccaaagcc gcgtttttgg agttcagccg
      961 tgccgcttgc ctcgactgta aacccacatc cattatcagt aatcgtgacg tgaagttcat
     1021 cgccgtccag ttgaatcgag acactggcgt cactcgcttt ggaatgtttg agaatgttgt
     1081 tgagggcttc ctgcacaatt cggtacaggc cgatttcacc ttcttttgag agcaacccgt
     1141 caatctgatc aatttcccag gtaaagccga tgtcagaggc attcgcaatt tgctgcagca
     1201 tggattgcag ggtcctggtc aaccccagcc gttccagttg atagggtcgc aggttgtgcg
     1261 caatctggcg aacttcttcg atggagagcg tagcggcgtc agagatttcg accaggtgct
     1321 cacgcatcgg attatcatcc tcagcagtgg tgagccccat cagagcatag tttttgatga
     1381 ccagcaggtt ttgtcccagg ctgtcgtgca attcggcggc aatgcgctgg cgttcctgtt
     1441 cctgtgaatc aatcaagcga cgcgaaaaag cctcctgcac gcggcgggca cgctcaacct
     1501 gattcagccg cactttgaat accagcccgg tgatacctgc cagcatcaga acgatcagtc
     1561 cgcgaaacca ccaggtttga taaaacctgg gcaagacaac aatctgcaat tccacaccac
     1621 tcagattcca caccccatca ctgttggcgg caatcacttt gaaggtgtac tcccctggcg
     1681 gcaggtaaga ataattcgcc atccggcgtg tgcccgcgtc aacccaatcc ggatcctgtc
     1741 cgatcaattg atacttaaag cgcatctgct ccggtttgat aaaactcagt ccagtatact
     1801 ggatttctaa attcgattgg cctggactga tgcggaggcc accctgaaaa gcagcgactt
     1861 cccggtcgag cctgcaggtt tcaatcaaca ccgacggagc gagcgagttc gttggtaccg
     1921 cttccggatc cacgatggca acaccttcct gagttggaaa ccagagcttg ccgtcgcggg
     1981 ctttgattcc cgccggctgc cgcccaccat tacattcaat attgagcatg ccatcatgtt
     2041 tgttataggc gatggaagta atggcctgga ttttcccagc ggcaaaatcg ttgagctgcc
     2101 ggcgactcac ccggtaaatt ccctgattgc aactcatcca gaaattcccg cgattatctt
     2161 cgagaatttg aaaaacccca ttgttatgaa gcccttcccg ggtcgtgtag caggtaaaac
     2221 gtccagtttg gtaccggatg agccccccat cataggtacc aaaccagagc acgccctcgg
     2281 catcctcata aatacagcgc atataatttg tggtcagtcc gtccgcgacc gtgaaaacgg
     2341 taaatttgcc attttccagc cgggcgatgc ctccgcccgt cgcaaaccac aacactccct
     2401 gacgatcctg atgaatggcc aagacatcac tacctggtaa tccatcttca acggtcagaa
     2461 aaaacgtctt cccatgctcg tatttgacca gcccacgatg tgttccgaac cacagcacgc
     2521 ccgagcgatc ctgataaatc gcaaaacaca tgtaatccat aaacgggatg ccgtcaggtg
     2581 gcatataaaa cctgccattc tgaaaaaaac ttggccttcc gcccatccat aaccgcccgg
     2641 cattatcttc acaaaaggcc tgcacattgg gtgagagcaa cccttcccgc acggtgtagt
     2701 tggtaaaact cccgtctttg taccggctca agccaccaaa cgccccgatc cagatactgc
     2761 cggcgcggtc ctggtaaatg ggatagacat tattaccaac cagtccattg gcggttgaat
     2821 aggtcgtaat aaattgatac gtcaaccggt ttagcccttg tccgttggtg ccgatccaga
     2881 gtgttccctc gcggtcttca aagatcgtcc tgatgatcat tgaggttggg taacttgaaa
     2941 atcgcccatt cttaaagtgg agcagcgcag ataaaccgtt gccggtcaaa tcttctgaac
     3001 taaaccagat atcgcctgcc cgatcctggc actgcgggag aatgagcttc ctggctggca
     3061 gcccatcctg cgaggtaaag tgcctgaaac gatccccagt aatcacgtag acaccatcgt
     3121 taaaagtccc aacccacaaa tttccttgct ggtcctcaaa cggatgcaga tgtttaaccc
     3181 catagttttt gaacttaatc ggaaaggtaa tccggtgacc agtcaagcga tggagcccct
     3241 cggcatccca ccgccagtcc gtccccgttg gaccggtaaa aaacaccatc gtactggaat
     3301 taagtcgttc tgggtcagcg atgaattgcc cttcgcgcca ataggcaaaa ccattctccg
     3361 tggtgatgag caattcaccg tttgactgac gctggatgtt gtgtatccaa ttatgtggga
     3421 gtccgtcagc cgtggtaaac gtggtaaaga caccatcgcg cagccgggtc aagccaccat
     3481 caatggtgcc aatccagagg gtcccgtcgg tatcttcaaa gagtgaggag aaacgattac
     3541 tggtcaaccc cctggaattg cctt
//
LOCUS       DAOQDO010000360         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19346_length_3564_cov_2.559134, whole genome shotgun sequence.
ACCESSION   DAOQDO010000360 DAOQDO010000000
VERSION     DAOQDO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19346_length_3564_cov_2.559134"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKZ53_07100"
     CDS             complement(<1..368)
                     /locus_tag="PKZ53_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40238.1"
                     /translation="MSITIVSNTESFVTTTMTIDPVAATGERQISVTTPVGTSNAISF
                     AINPGNTGCGEITITLIGSSGDVGAILPTAGTDYLVGGGNGGGTKASFAGTASFEIDL
                     TQVSGPVEIKTGWDLEAQSLG"
     gene            complement(432..1175)
                     /locus_tag="PKZ53_07105"
     CDS             complement(432..1175)
                     /locus_tag="PKZ53_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40239.1"
                     /translation="MTEPIRIPECCPLCAEFPKSVEIPNCWNCGYEFPLSYVIPYAEQ
                     WDLTDELLMEVSKYQGLRLLGKFVGKQRNVISNFHQIKYLYLSRYRGFQFAFLKDQKV
                     LNTIELDLTPIFSLDGVEEIQNLSVLKLIECRKLENIEALSKSQSIKVLDLRLCNRIS
                     DFSPVGTMSQLRVFCTDSHKMDSFDFLRNLPELNTLGLFGDKKVKLEVEIFQNLPHLK
                     FFSSRRKFLSKAAIEKLKTWCPNCEIILF"
     gene            complement(1211..2005)
                     /locus_tag="PKZ53_07110"
     CDS             complement(1211..2005)
                     /locus_tag="PKZ53_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ40240.1"
                     /translation="MEFLIYDYFFWLAVFALVFVVVERVRPTRARQIVRAGFWTDVGH
                     FLFNAKVFGMLTAAVIPFLIESFENWLDRFGLYGWIYRGVVEDWPFWAQFLSVLFGLD
                     LCKYLIHNLLHRVPWLWEFHKVHHSLTEMDWIGNWRFHWLEAVMYDTLLYVPVVYLGC
                     SGYVLFWYAVIGTLVGQYAHANLQLPIGPLKYLINHPELHIWHHVHPDSGPLNRNFGI
                     VLCCWDWIFRTAYLPDQKVPARLGFDGIEAFPKTFWFQQAWPLRKG"
     gene            2423..>3564
                     /gene="egtB"
                     /locus_tag="PKZ53_07115"
     CDS             2423..>3564
                     /gene="egtB"
                     /locus_tag="PKZ53_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HNJ40241.1"
                     /translation="MSNHVFGQPQLASPTPKDQLFEEFQKVRTLSETLCLPLETEDYV
                     IQSMPDVSPPKWHLAHTSWFFETFILQSFAKDYTCFHPKYNFLFNSYYEAVGERHPRP
                     KRGLLSRPTVDEVMQYRHFVDAGMQRFFETLDDTTFETVKPLLTLGLHHEQQHQELLL
                     TDIKHILAINPLRPAYKANFHQPAEETTTDVPRLEWIGFSGGVKQIGYAGHDFVFDNE
                     TPRHSVFVEDFKLASRLVTNGEFLEFIADGGYCTPTLWLSDGWRVILEQGWDSPLYWE
                     RIEGDWWMMTLAGMRRVDPAEPLCHVSFYEADAFARWTGKRLPTEAEWEYAAEGIIVS
                     GNLLESGTLHPTALSSTFPGSTGKPQQLFGDVWEWTQSDYRPYPGF"
BASE COUNT          954 a          944 c          794 g          872 t
ORIGIN      
        1 cccagggatt gggcttccag gtcccacccg gtcttgattt ccaccggccc gctcacctgc
       61 gtcaggtcaa tctcaaagct ggccgtccct gcaaagctgg ccttggtccc gccgccattc
      121 cctccaccca ccaggtagtc agtgcctgca gtcgggagaa tcgcccccac atccccgctt
      181 gacccgatca aggtaatggt gatctctccg cacccagtat ttcctgggtt gatggcaaat
      241 gaaatcgcat ttgaagtgcc gaccggcgtg gtgacactta tttgccgctc gccggtcgcg
      301 gcgactggat caatcgtcat cgttgtcgtc acaaatgact cggtgttgct cacaatcgtg
      361 attgacacac cttccggagg tgggtagtag cctggaataa tctcaggcta ttccgttgca
      421 ccgctttcca cttaaaacaa aattatctca caatttggac accatgtttt gagcttttca
      481 attgccgctt tagacagaaa ttttcggcgg gaactgaaga atttaagatg aggtaagttc
      541 tggaaaatct caacttccaa ttttaccttt ttatcaccaa acaaccctaa tgtattcaat
      601 tctggaagat ttcttaaaaa atcgaatgag tccattttat gagaatcggt gcagaacact
      661 ctcagctgtg acattgtgcc aactggagag aaatcactaa tacggttaca caacctcaaa
      721 tctaaaacct tgatagattg agatttactc aatgcttcga tattttctaa tttccggcat
      781 tcaatcaatt tcaaaacaga tagattctga atttcctcaa ctccatccag tgaaaagatc
      841 ggtgtaaggt caagctcaat cgtgttgagg actttttgat ccttaagaaa tgcaaattga
      901 aaccctctat agcgactcaa atacaagtac ttgatctggt ggaagttaga aatcacattc
      961 ctttgtttac caacaaattt tcccagaagt cgcaacccct ggtacttcga aacttccatt
     1021 aagagttcat cggtgagatc ccactgttcc gcatatggaa tcacatagga aagagggaat
     1081 tcatatccac aattccaaca atttggtatt tcaacagatt ttggaaattc cgcacaaagc
     1141 ggacagcatt cggggatacg aattggttca gtcatgtaaa cctctttttg ttctcaaatc
     1201 aggaatttgg ttatcccttt ctcaacggcc acgcttgttg aaaccagaag gtctttggaa
     1261 atgcctcgat accgtcaaaa ccaagtcggg ctggaacctt ttgatctggt aaataggccg
     1321 ttctgaaaat ccagtcccag cagcaaagga caattccgaa attccggttc aaaggaccgc
     1381 tatcgggatg cacgtgatgc cagatgtgaa gctccgggtg gttgatcagg tatttcaacg
     1441 gcccaatcgg aagctgaagg tttgcgtggg catattgccc aaccagcgtt ccgatgaccg
     1501 cataccagaa cagcacgtag ccgctacacc ccagatacac gactggcaca tagagcaagg
     1561 tgtcgtacat caccgcttcc agccagtgaa agcgccagtt ccctatccag tccatttcgg
     1621 taaggctgtg atggactttg tggaattccc acaaccacgg aactcgatgc aacaggttgt
     1681 gaatcaggta cttgcacaaa tccaacccaa acaaaacgct caaaaattga gcccagaacg
     1741 gccagtcttc caccacgccg cgataaatcc atccatagag cccaaaccgg tcaagccagt
     1801 tttcaaacga ttcgatcaga aatggaatca cggcagccgt caacattcca aacactttcg
     1861 cattgaacag aaagtggccc acatcggtcc aaaaaccggc ccgcacaatc tgacgcgcac
     1921 gggtcggacg cacccgctct accaccacaa acaccagggc aaacaccgcc agccagaaga
     1981 aatagtcgta gatcagaaat tccataaacc ttcatagtca aagtacaaaa gcggcgtcaa
     2041 gtcgccgcac tccaaattgt gaaagcggcg tcaagtgggc cgcactccaa attgtgaaag
     2101 cggcgtcaag tgggccgcac tccaaatttt ggtttctttg cgacgccgag ttggtgcaca
     2161 atccctaaca tccagagcaa ctaccgtgat ttcacattct tataccattt tggaatgaag
     2221 tagtcatatt aggaaccagt caggtcgttc agtcaaatgg acttagtgaa cgactgacta
     2281 ctggctactg actactgact ggtattacac attactgatt ttggttcaga gttcaacccg
     2341 atctatcagc ttttaagaaa ccgatttctg aactttttct caaaaggttg aaccccgaac
     2401 gctcgaccag gagaccaatc cgatgagcaa ccacgttttt ggtcagcccc aactcgcctc
     2461 gcccacgccc aaagatcagc tttttgagga atttcagaaa gttcgcacct tgagcgaaac
     2521 cctgtgcctg ccgcttgaaa ccgaagatta tgtcatccag tcaatgccgg atgtcagccc
     2581 gcccaagtgg catctggccc atacaagctg gttttttgaa acctttatcc tgcaatcatt
     2641 tgccaaagac tatacctgct ttcacccaaa atataatttt ctgtttaatt cctattatga
     2701 agcggttggc gaacgtcatc cgcgccccaa acgaggattg ctgtcacgcc caacggtgga
     2761 tgaagtcatg cagtaccgcc actttgttga tgcgggaatg cagcgctttt ttgaaacgct
     2821 cgacgacacc acgtttgaaa ccgtcaaacc actcctgaca cttgggttac accacgaaca
     2881 acaacatcag gaactgctgc tgaccgacat caaacacatc ctggcgatca acccgctccg
     2941 cccagcgtac aaagccaatt ttcatcaacc ggctgaagaa acaaccaccg atgtacctcg
     3001 gctggaatgg attgggtttt caggcggcgt caaacaaatt ggatatgctg gccatgactt
     3061 tgtgtttgac aacgaaaccc ctcgccattc ggtgtttgtt gaggatttca aactggcgtc
     3121 acggctggtc accaacggcg agtttttgga atttattgct gatggcgggt attgcacacc
     3181 tacgttgtgg ctttcagatg gctggcgcgt gattcttgag caaggttggg attcaccgct
     3241 ctactgggag cgcattgaag gcgactggtg gatgatgacc ctggccggca tgcgccgggt
     3301 tgacccggct gaaccactgt gccacgtaag tttttatgag gcggatgcct ttgcccgctg
     3361 gaccggaaag cgcttgccga ccgaagccga gtgggaatat gccgcagagg gcatcatcgt
     3421 ttctggaaat ttacttgagt caggcacgct tcatccaacg gcgctgtctt caacgttccc
     3481 aggctcaacc ggaaagccac agcaactctt tggtgatgtg tgggagtgga cgcagagcga
     3541 ttaccgcccc taccccggct tcaa
//
LOCUS       DAOQDO010000361         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19355_length_3562_cov_3.197605, whole genome shotgun sequence.
ACCESSION   DAOQDO010000361 DAOQDO010000000
VERSION     DAOQDO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19355_length_3562_cov_3.197605"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1900
                     /locus_tag="PKZ53_07120"
     CDS             335..1900
                     /locus_tag="PKZ53_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ40242.1"
                     /translation="MSISETTQADILVVDDNALNIRVLVEILQRRGFSVQSATNGPDA
                     LKMAQENPPDLVLLDVLMPVMNGYQVCRLLRANAATQNTPIIFLSALNEPLDKVEAFN
                     VGATDYISKPFNAREVLARIESQLKIQGLRKELEAKQRDLERRNEELARKNEELMQSQ
                     RALMKAHRNINLVFSVFTDALLGTVLDDKYLLEEKIGEGGFGSVYRATQIELQRPVAI
                     KVFRPSDLYPQSPEAQLERFRMEGISACRIQHPNAVSVLDFGITSAGIPYLVMELLNG
                     RTLATELDDKKSLSIKRCAEIVIPVCHLLTKAHEMGMVHRDIKPDNIFLHQSDGVEII
                     KVVDFGIAKLLVDSSEVEYRSNLTITGNLVGTPVYMSPERLKGSAYGVQSDIYSLGIT
                     LYQILCGRLPFETSKTEIYNLVAWHLTQKPRPLRDFNPLIPESLEDLVLRMLLKKPEL
                     RPTAREVTEALHQMVDQLPDTPVVTFFDFPPTLSPNFDEDESETSIPPHQTRPTNQQI
                     STPITQENAKLVE"
     gene            2236..>3562
                     /locus_tag="PKZ53_07125"
     CDS             2236..>3562
                     /locus_tag="PKZ53_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ40243.1"
                     /translation="MERAEILVVDDNPLNIKILSSLLNKHGYVVRAAHSGEEALLEVS
                     QSHPDLILLDIMMPDLSGYEVCCQLRKNTSTAKIPIIFLSALHEPHEKVEAFRSGGND
                     YIMKPFHAEEVIARIENQLNIQQLRRELESSKRNLEQQNRELASKHEELVKSHQALVQ
                     AQRHIDLYFSVFTNALQGTIFDNKYELEQKIGEGGFGTVYRATQIGLQRPVAIKIFRP
                     SEPNLQAPDKQVDRFRREGIAACRIQHINAVSVLDFGITSAGIPYLVMELLNGKTLNQ
                     ELSESSHLTLQRCAEIIIPICNLLLKAHEMGLVHRDIKPDNIVLHQSDEGEIVKVVDF
                     GIARLVEDNSGANILNTLTVSGNVVGTPLYMSPERLKGTPCGSPSDIYSLGITLYQML
                     CGELPFESVKTDLYSVVTWHLTMKPSPLRGINPFVPLEIEDLVLRMLFKL"
BASE COUNT          944 a          883 c          818 g          917 t
ORIGIN      
        1 gtcttcagcc ccaagcccga tgtcttcagc cccaagcccg atgtcttcag ccccagtttt
       61 ttcatctcct acttgctgtc gccgttaagt ttcttgaaaa ccggaaactt aacggctatt
      121 tttgttttgt ctcttgttta ggtttggcgc ttcccctcaa tgagccagtt gcctgaaaaa
      181 ccagggttta tcacattctt gaaagaaatt ggcatttcag tcacttatta tattccatct
      241 gattttcaat tgactttcaa aacaccaaga cgttcctgac tttgccggtt gtggcctgta
      301 cggttcgtca acgtccagga cgaggaattt ctccatgagt atcagtgaaa caacccaggc
      361 cgatattctt gtggttgatg acaatgccct caatattcgg gtgctggtcg aaattctgca
      421 gcggcgtgga tttagtgtcc aatcagccac gaacggaccg gatgcgctga aaatggccca
      481 ggaaaatccg cctgacctgg tattactgga tgtcttgatg cccgtcatga acggatatca
      541 ggtatgtcgg ttgcttcgag ccaatgcggc cacacaaaac acacccatta tttttctgag
      601 tgccctcaac gaaccactgg ataaagtcga ggcatttaat gttggtgcca ctgattacat
      661 ctctaagccg ttcaatgcac gggaagtatt ggcccggatt gaaagtcagt taaaaatcca
      721 gggtttgaga aaagagcttg aagccaaaca acgtgacctg gagcgacgca atgaagagct
      781 ggctcgcaaa aatgaagaac tgatgcaatc acaacgggcg ttaatgaagg cccatcgcaa
      841 catcaacctg gttttttcgg tctttaccga tgccctgctg ggaaccgtcc tggatgacaa
      901 atatttactg gaagaaaaaa tcggggaagg tgggttcggc agtgtctatc gtgccaccca
      961 gattgagttg caacgtccgg tggccatcaa agttttccgc ccctcagacc tctatccaca
     1021 aagccctgag gcacaactcg aacgatttcg gatggaggga atttccgcct gccggatcca
     1081 acacccaaat gcagtctcag tgcttgattt tggaattact tcagccggaa ttccatatct
     1141 ggtcatggag ttgctcaatg gccggacgct ggcaactgaa ctggatgaca agaaatcact
     1201 ttcgatcaaa cggtgtgcag agattgtgat tccagtttgt cacttattaa cgaaagccca
     1261 cgagatggga atggtccatc gggatatcaa gccggataac atctttctcc accagtctga
     1321 tggagtggaa atcatcaaag ttgtggattt cgggattgct aaactgctgg ttgactcatc
     1381 tgaagttgaa taccgatcaa atttgacaat cactggaaat ctggttggga ctccggtgta
     1441 tatgtctcca gagcggttga aaggctcagc ctatggggtt caatctgata tttacagcct
     1501 tggaataacg ctctatcaaa tcctgtgcgg acgattgccc tttgaaacca gcaaaaccga
     1561 gatctataac ctggtggcct ggcatttgac acaaaaaccg cgcccattac gcgatttcaa
     1621 tccattgatt cctgaatcac tcgaagacct tgtccttcgc atgttgctga aaaaacctga
     1681 attacggcca actgcccgtg aagtgaccga agcgctccat cagatggttg atcagcttcc
     1741 tgatacccca gttgttacat tttttgattt tcccccgacc ctgtcaccaa attttgatga
     1801 agacgagtcg gaaacgtcca tcccccctca tcaaacccgc cccaccaacc agcagatatc
     1861 aactccgatc actcaggaaa atgcaaaatt agttgaataa ccagggcttg ccccactgct
     1921 ggacggttga ggaaccattg cgccggcaac accgccagaa attcaggttc cggaccagcc
     1981 accttccttc tgcaatttcc accagccgtt ctgcactttt ttctgatatg atatttggga
     2041 ttcggcctcc ttttgattcc tacggattcg cttcaatttc ggcatcattt gactcattgt
     2101 cttcaaccag gtctgctggg ctatggtgtg tgaagagtgg gttgggaaat gcccaggttt
     2161 ttcctcctac ctgtcctgaa agaggaactc ccataaattc aacaaaaaga atggtctggt
     2221 atggaaataa tcaatttgga gagagctgag atcctggttg ttgacgacaa cccgctcaat
     2281 attaaaatcc tctcgtccct tttgaacaaa cacgggtacg tggtgcgagc tgcccatagc
     2341 ggggaagaag ccctcctgga ggtctctcaa tcgcacccgg atttaatcct gcttgacatc
     2401 atgatgcctg atctgagcgg ctatgaagtg tgttgccagt taagaaaaaa cacgagcacg
     2461 gccaaaattc cgattatttt tctgagcgca ctccacgaac ctcacgaaaa ggtcgaagcc
     2521 tttcgttctg gaggaaatga ctacatcatg aagccatttc atgcggaaga agtcattgcc
     2581 cggattgaaa atcaactcaa catccagcag ttgcgccggg aactggaatc cagcaaacgc
     2641 aacctggaac aacaaaatcg agaactggcc agcaaacacg aggaactggt gaaatcccat
     2701 caggcactgg tgcaggctca acgccatatt gacctgtact ttagtgtgtt caccaatgcc
     2761 ttacagggaa caattttcga caacaagtat gaacttgagc aaaaaattgg tgagggtggt
     2821 tttggaaccg tctatcgggc cacacagatt ggcttgcaac gacccgtggc gattaaaatc
     2881 ttccgcccat cagagcccaa cctccaggct ccggacaagc aggttgatcg ctttcgccgt
     2941 gaaggaattg cagcctgtcg catccaacac ataaatgccg tttcggtgct tgattttggc
     3001 attacatcgg ctggaatccc gtatctggtc atggaattgt taaatggcaa aacgctgaat
     3061 caagaactga gtgaatcttc gcatctgaca cttcagcgat gtgctgaaat catcattcca
     3121 atctgtaacc tgctgctcaa agcccacgaa atgggactgg ttcaccggga tatcaagcca
     3181 gacaatattg tgttgcatca gtcagacgaa ggggaaatcg tcaaagtcgt tgattttgga
     3241 attgccagac tggttgaaga taattcaggg gcaaatattc tcaatacact cacggtttcc
     3301 ggaaatgttg ttggaacacc actgtatatg tctcccgaac gactcaaagg gacaccctgt
     3361 ggcagcccat ctgatatcta cagcctggga atcaccctgt atcaaatgtt gtgtggtgaa
     3421 ttaccgtttg agtcggtgaa aaccgatctg tattcggtcg tcacctggca tctgaccatg
     3481 aagccctctc cactccgggg aataaacccg tttgtcccac tggaaatcga ggatctggtt
     3541 ttgcgaatgc tctttaagtt ac
//
LOCUS       DAOQDO010000362         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19445_length_3552_cov_2.791250, whole genome shotgun sequence.
ACCESSION   DAOQDO010000362 DAOQDO010000000
VERSION     DAOQDO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_3552_cov_2.791250"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..>3552)
                     /locus_tag="PKZ53_07130"
     CDS             complement(21..>3552)
                     /locus_tag="PKZ53_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40244.1"
                     /translation="TTRAVTSYRPEPQNPNSLSHLKVTALCTDQQGRIWVGTDGGGVC
                     QFDQTTKSFQVFRANPKAPTGLSVDRIWTIYSDRKGQIWVGTWGGGLCRYLPEQNGFL
                     AYQNHPQRLNSLSSNIVWAIYEDRAGLLWIGTFGGGLCCYDPNLQRFPTFSSDPEDPK
                     SLPSNNIFAFAEDPDRNVWVGTFEGGLSKFDPATGQVTTYRHKDGDARSLPNDNVWSI
                     CPDRQGHLWLATFNGGLSQFDLTSQTCTSFASNPKFPNGLPGNDTRLVFCSRDGTVWV
                     GVRGFGLGRFLPERNEFIPIASEIPGLPSLKEARALYEDQAGILWLGSELGLIAIDRT
                     RKTFEWFSHNPNDSLSLCSNLVWSVTEAPNGLLWIGTKDGLSCFDRTQRVFYSFREAE
                     GLPNNAVYGILPDSAGNIWISTNVGLSRLTPKLEAGRWSVKTWNFDVGDGLQSNEFNM
                     GASLKTSDGKMLFGGVNGFNLFDPAQIQRNEYLPPVVVTAFRKFNSLVKLETAISETR
                     DIEINYKDNFISFEFAGLSFSNAGKNQYAFKLEGFNEDWIPAGSHRVATYTNLDGGDY
                     VFRVKASNHDGVWNETGAQLRIRVIPPIWKTRWAYFFYIFGGSALMYGGVRYRLKRLE
                     RQNRELEQKVAERTAALDEANTELAHKVDQLDLANQETERKNQELDQKVLELNRKNEE
                     LIASQQRADRIFSALAEALPGTVLEGKYRLEEKIGSGGFGAVFRATHLALNRMVAVKV
                     FRPSRGNDSAEAVERFRLEGVSASRVNHPNAISVLDSGISTEGIAYLVMELLNGHSLA
                     TELKRNKILSLQRTAQILTPVCAALAEAHRIGIVHRDIKPDNIFLHRAPEGEIVKVVD
                     FGIAKLVEADPGGEYQHLTATGGIVGTPVYMSPERWGNRPYDGRSDVYSLGIMVFQML
                     TGKLPFVAESNSMVNLVMAHMNKKPPTIREYNSAIPEKVEAVVMQTLEKDPDQRPTAR
                     EFAERFESVLEILVHQQGEATRKFDSGFQQIWGMGQTTAHEFGQRSTAPDVPKIQTSA
                     YSEVTLAETQIENPMESPTFVELPGDQQTVDDIRHTGNGGDYPTVVDAQDIVASSGDS
                     ETIVEKLELPKPTAKPLFSHGSETVEGPIEGFPPKPAQPPSFDTVEGPVEKFRAQPVA
                     PQTLKPGHGIKRDIQSDSSQESPSDFPTIG"
BASE COUNT          869 a          951 c          850 g          882 t
ORIGIN      
        1 gtgtcggcct ggaactgacg ttaaccgatt gtaggaaaat cagatgggga ctcctgagag
       61 gaatccgatt gaatgtcgcg tttgatgccg tggccgggct tcaacgtttg cggcgccaca
      121 ggttgagcac gaaatttttc aactggacct tcgacggtat caaatgatgg aggttgagcc
      181 ggttttggcg gaaaaccttc aattgggcct tcgactgttt cagaaccgtg ggaaaataac
      241 ggtttggcgg tgggtttggg cagttcgagt ttctcgacaa tggtttcact atccccagat
      301 gaggcaacaa tatcctgggc atccacaact gttgggtaat caccaccgtt cccggtatgt
      361 ctgatgtcat ccacagtttg ctggtcacca ggaagttcca caaacgtcgg actctccatc
      421 ggattctcaa tctgggtttc agccagtgtc acttctgaat aggcactggt ctggatcttt
      481 ggaacatccg gtgcggttga gcgctggcca aactcatgcg ccgtggtctg gcccattccc
      541 cagatctgct gaaatccact gtcgaattta cgcgttgctt caccttgctg gtgaacgagg
      601 atctcaagaa ctgattcgaa gcgctcggca aattctcttg ccgtgggacg ctgatcagga
      661 tctttttcta aggtctgcat cacaaccgcc tcaacttttt cgggaatggc agagttgtat
      721 tcccggatcg tcggtggctt tttgttcata tgcgccatga ccaggttgac catactgttg
      781 gattccgcca caaagggcag ttttccagtc aacatctgaa acaccataat tccaaggcta
      841 tacacgtcag agcgtccatc atacgggcga tttccccatc gttcaggtga catatacacc
      901 ggcgttccga caatcccacc cgttgcggtc agatgctgat actcaccgcc ggggtctgcc
      961 tcgaccagtt tggcaattcc aaaatcaacc actttgacga tctcgccttc cggcgcccga
     1021 tggagaaaaa tattgtccgg tttaatgtcg cgatgaacaa tgccaatccg gtgagcttca
     1081 gctaaagcag cacacaccgg cgtcaaaatc tgggctgtgc gttggagtga aagtatttta
     1141 tttcgtttca attcagtcgc cagtgaatga ccgttgagca actccatcac cagataggca
     1201 ataccttcgg ttgaaatgcc cgaatcaagt acggagatgg cgtttggatg attaacccgc
     1261 gaagctgaca ccccttcaag tcgaaatcgc tccaccgctt cggcagagtc attcccgcga
     1321 cttggacgaa agaccttgac ggcaaccatt cggtttaagg ccagatgagt tgcccgaaac
     1381 acagcgccaa acccgccgga gccaattttt tcctccagcc gatacttccc ttccaacacc
     1441 gtaccgggca aggcttcggc cagggcagag aaaattcgat ccgcccgctg ctgcgaggca
     1501 atcaactcct cgtttttgcg attgagttcc aggactttct gatccagttc ctggtttttg
     1561 cgttcggttt cctggttggc tagatccaac tgatcaactt tgtgggccag ttcggtattg
     1621 gcctcgtcga gcgcggcagt ccgttcagcg actttctgtt ccagttcgcg gttttggcgt
     1681 tccagccgtt tcaggcgata ccgaacgcca ccatacatca aagctgaacc gccgaaaata
     1741 tagaagaaat aagcccatct ggtcttccaa atcggaggta tcacgcggat tcgaagctgt
     1801 gctccggttt cgttccagac accgtcgtga ttgctcgctt tgacccgaaa aacatagtca
     1861 ccaccgtcca ggttggtata ggttgcgacc cgatggctgc cagccggaat ccaatcttcg
     1921 ttaaatcctt cgagcttaaa ggcatactga ttttttccag catttgaaaa actcaacccg
     1981 gcgaactcaa aggagatgaa attgtctttg taattgattt cgatatctct ggtttcagaa
     2041 atcgccgttt ccagcttaac cagactgtta aactttcgaa acgccgtcac caccactggc
     2101 ggcagatact catttcgttg aatctgggcg ggatcaaaca ggttaaaccc attgacaccg
     2161 ccaaagagca ttttcccatc gctggttttg agcgaagcac ccatgttgaa ttcattgctc
     2221 tgcaacccat cgccaacgtc aaaattccag gtcttgacgc tccaccgacc agcttccagt
     2281 tttggtgtca gccgtgagag cccgacattg gtactaatcc agatgttgcc ggcactgtct
     2341 ggcaaaatgc catagacagc gttgtttggg agcccttcag cttcacgaaa agaataaaaa
     2401 acccgttggg ttcgatcaaa acaactaagg ccatctttgg tgccgatcca cagtaacccg
     2461 tttggagctt cggtgaccga ccagaccaga ttgctgcaca agctcaacga atcattaggg
     2521 ttatgcgaaa accactcaaa cgtcttgcgg gtccgatcaa ttgcaattaa cccaagctcc
     2581 gacccaagcc agagaatacc agcctgatct tcatacagtg cccgagcttc tttcaaactt
     2641 ggaagacctg gaatctcaga tgctatcggg ataaattcat tccgctctgg aagaaatcgc
     2701 cccagtccaa acccacgaac accaacccag accgtaccat cccgtgaaca gaaaaccagt
     2761 cgagtgtcat ttcctggcaa cccattcgga aactttgggt tactggcaaa actggtacag
     2821 gtttgactgg tcaggtcaaa ctgtgacaag ccaccattaa aggttgcaag ccagaggtgc
     2881 ccctgccggt ccgggcaaat tgaccagaca ttgtcatttg gcaagctgcg agcatctcca
     2941 tctttgtgcc ggtaggttgt cacctgccct gttgctggat caaattttga caatccgcct
     3001 tcaaacgtcc caacccagac gtttcgatca ggatcttcgg caaacgcaaa aatattgttt
     3061 gatggaagtg actttggatc ttccggatca cttgaaaagg tcgggaagcg ctgtaaatta
     3121 ggatcgtagc agcacaagcc tccgccaaaa gtgccaatcc agaggagccc ggcacggtct
     3181 tcataaatag cccatacaat attgctgctc aggctgttta gacgttgagg gtgattttga
     3241 taggcaagaa acccattttg ttcaggaaga taccggcaca aacctcctcc ccaggtcccc
     3301 acccaaatct gccctttccg gtctgaatat atcgtccaaa ttcgatcaac actgagcccg
     3361 gtcggagctt ttggattggc ccgaaaaacc tgaaaagact ttgttgtctg atcaaactga
     3421 caaacgccac caccatcggt tccaacccag atgcgtcctt gctggtccgt acagagtgcg
     3481 gtgactttca ggtgactgag cgaatttggg ttctgaggtt cgggacggta actggtcacg
     3541 gcacgggtgg tt
//
LOCUS       DAOQDO010000363         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19462_length_3550_cov_2.794278, whole genome shotgun sequence.
ACCESSION   DAOQDO010000363 DAOQDO010000000
VERSION     DAOQDO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19462_length_3550_cov_2.794278"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..856)
                     /gene="kdsB"
                     /locus_tag="PKZ53_07135"
     CDS             complement(41..856)
                     /gene="kdsB"
                     /locus_tag="PKZ53_07135"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNJ40245.1"
                     /translation="MKSNRFSTPKQHAHSVVAILPARYASSRFPGKPLVDLCGKPMVQ
                     RVYEQAQQAQTVSRVLVATDDERIQTAVLNFGGEVVLTRPDHPTGTDRLAEVAAGLSE
                     SIIVNVQGDEPLMSPATIDAAVQPLLDDPTLEMATTCEPLTQGEDIINPNIVKVVCNQ
                     QGFALYFSRSVIPALRLPNPLSESVTPPDATSAHPADVWFKHTGLYVYRREFLLHFAS
                     LSPCFLEQLESLEQLRALYHGHRIKVVKVSHASPGVDTPEDADRVRALLNSIT"
     gene            1021..1971
                     /locus_tag="PKZ53_07140"
     CDS             1021..1971
                     /locus_tag="PKZ53_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40246.1"
                     /translation="MYPFRRPGGFFEIAPYRDIFDGSELGQERMRLWKTYLHEGRGFL
                     EILHLWAEYPTFRALVDQVRTNNRLQAISVAWNDWASDHEDDARSFITAQSTHVWTTL
                     DSTAKATFLAITHALMREGLMDQVLFVTEIRGKLSGIGTTFRLYCFLQSTAPARIQPF
                     FGKSCKGVNYFYEPLHPICYRQSQSAPSFEISTREHQTTLKRKDGTVKGWESDIVIDY
                     REVEFTHLSPDNFDITALSGSHSHFQLHEIRFGGEVPGLNQFRPQMLEALEAQPEGPV
                     ARPEPLPSFYDVGIDPSSVAVAEPEAPQSEEPKDILDQID"
     gene            complement(2315..2830)
                     /locus_tag="PKZ53_07145"
     CDS             complement(2315..2830)
                     /locus_tag="PKZ53_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40247.1"
                     /translation="MKHLIQLLKTPVFAGGVLFVAFFFASLPANAQNWRYEDDNYRPS
                     ESWVRQQGREKGYQLGTREGAQDRVQGNRYDYSDETYDYALNGFKPQWVHDDNYKKGF
                     REGYKQGYENGYQNPRSVLNDWRYGNNNRRNDDYYRNSNNGRGRDWNRGRDQHCDRDD
                     DYYRRDNRRRF"
     gene            3035..3232
                     /locus_tag="PKZ53_07150"
     CDS             3035..3232
                     /locus_tag="PKZ53_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40248.1"
                     /translation="MKFPVHTISTEWVAFAEPYGVACFWLPEPKKIISVFLENLEMYG
                     NQKLTDTSPFFGRENAENQFV"
     gene            3210..3500
                     /locus_tag="PKZ53_07155"
     CDS             3210..3500
                     /locus_tag="PKZ53_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ40249.1"
                     /translation="MQKINLFETRNFFADIYCLEPGQAQKAHAHAGADKIYAVMEGKV
                     VALIGEEEQELSANDAVLAPADVIHGIRNASSERAVVFVFMSPNPNQAKENQ"
BASE COUNT          871 a          868 c          912 g          899 t
ORIGIN      
        1 gaatccacag gtgcatcgta cctggcagta ttttgttcaa tcaggttatt gaatttaaaa
       61 gggctcgcac gcggtcagca tcttcgggcg tatcaacgcc gggcgacgcg tgggaaactt
      121 tcacgacttt gatgcggtga ccgtgataga gcgcccgcaa ttgttcgagt gattcaagtt
      181 gctctaaaaa acacggcgac aacgatgcaa agtggagcaa aaattcacgc cggtagacat
      241 agagtcccgt gtgcttaaac cagacatcag cgggatgagc cgaggtggca tctggcgggg
      301 ttaccgattc gctcagtgga ttgggcagcc ggagcgccgg aatgaccgac cgtgaaaaat
      361 acagggcaaa tccctgctga ttgcacacaa ccttgacgat gtttggattg ataatgtctt
      421 cgccctgggt gagcggctcg catgtggtgg ccatctcaag ggttggatcg tccaggagtg
      481 gctgaaccgc agcgtcaatc gtcgccggcg acatcagtgg ttcatcaccc tggacgttga
      541 cgatgatgga ttccgaaagc ccagccgcca cttcggccag ccgatcagtg ccagttggat
      601 gatcaggtcg agtcagaacg acttcaccgc caaaatttag tacggcggtc tgaatgcgtt
      661 cgtcgtcagt tgccaccagc acccggctga cggtttgcgc ctgctgggcc tgttcgtata
      721 cccgttgcac catgggtttg ccgcacaaat ccaccagcgg ctttccggga aagcgtgacg
      781 aggcatatcg agcaggcaga atggcaacaa ctgagtgggc gtgttgtttt ggagttgaga
      841 agcgattgga tttcacggga tgccacctta gcccagactc tttgcgcttg gcaagtggcc
      901 atagacccat ttcatgggct tcccgttctg tcgttgaagg cttatacttt ctgccctttc
      961 attttttccg cttttcgatc aacacaacat tatccacacc aattcgggag aatcagtgcc
     1021 atgtatccct ttcgcagacc gggaggattt tttgaaattg cgccgtaccg tgacattttt
     1081 gacggcagtg aacttggaca ggaacgcatg cggctctgga aaacctattt gcacgaaggt
     1141 cgcggctttt tggagatttt gcacctctgg gctgaatatc caacgtttcg cgcgctggtg
     1201 gaccaggtcc gcaccaataa ccggctgcag gcaatctcag tggcctggaa tgactgggct
     1261 tctgatcacg aagacgatgc ccgatcattt attaccgccc agtccactca cgtctggacc
     1321 acactggatt caaccgccaa agcgacgttt ctggccatta cccacgcctt gatgcgcgaa
     1381 ggtctgatgg atcaggtact gtttgtgact gaaattcgcg gaaaattgtc cgggattgga
     1441 accacgtttc gcctgtattg ttttttgcag tcaacggcac cggctcgaat ccaacccttt
     1501 tttggcaagt cctgcaaagg cgtgaactat ttttatgaac cgttgcaccc gatttgctac
     1561 cgccagtccc agagtgcgcc cagttttgaa atttcaaccc gtgaacacca gacaacgctc
     1621 aaacgcaaag atggaacggt caaaggctgg gaatcagaca ttgtgattga ctatcgggag
     1681 gtcgaattta cccatctgtc ccccgataat tttgacatta cggccttaag cggcagccat
     1741 agccattttc agttgcacga aattcgcttt ggtggcgaag taccggggct gaaccagttt
     1801 cggccacaga tgctcgaagc gctggaagcc caacctgaag gtcccgtggc ccgtccggaa
     1861 ccattgccgt cattttatga tgttggaatt gacccgtctt cggttgctgt ggctgaacct
     1921 gaagctccgc aatctgaaga accaaaagac attctggatc agattgattg aaaaaccagg
     1981 gctgaaaact ttgggctgaa gacatcgggc ttagggctga agacattggg ctcagggtgt
     2041 tgaacccgtt ttcttcagcc ccgagcttgc gagtcttcag ccccaagccc tcagccccag
     2101 gatttttcag cccacggtct cttaaaaaca aaaaaaggcg tgaagccata tccccaaagc
     2161 ttcacgccgt tcgaagtttc caaaggctct tgagagagaa tccaaggaaa gacaaatcga
     2221 aaaatattgt ggagtggagg tgtggctgtc agtgtttagc ctgtcccatt ccgccttaga
     2281 aggaacttac cgaggaccac acctgactcc acggttaaaa tcggcggcga ttgtcgcggc
     2341 gatagtaatc atcatcgcgg tcacaatgtt ggtcacgacc acggttccaa tcccggccac
     2401 ggccattgtt gctgttgcgg tagtaatcat cattgcgacg attgttgttg ccatatcgcc
     2461 agtcattgag aacgctgcgc gggttctggt agccattttc atagccctgt ttgtaccctt
     2521 cgcggaatcc ttttttgtag ttatcgtcgt gcacccactg tggtttgaag ccgttcagcg
     2581 catagtcata ggtttcgtct gaataatcgt atcggttgcc ctgaacgcga tcctgagcac
     2641 cttcacgggt accaagctga tagccttttt cgcgtccttg ctgacgaacc catgactcag
     2701 aagggcggta attgtcatct tcatagcgcc agttttgtgc gttggcaggg agtgaagcga
     2761 agaaaaatgc tacgaataat acccctccgg cgaatactgg tgttttcaat aattgaatca
     2821 aatgtttcat tgtgatgcct ccaaattgtt gatctcttgt gttcagtgct ttgcctgcgg
     2881 gttggattgg tccaactctg atcacccacg tgtaatgtgt tttgcccttc tctatgtcaa
     2941 tcggtgtgcc acagtttcca gatcgctgca aagttgtgtg aactaactgg aaagaaacaa
     3001 tttgtgcctg aaattcgatg cctggaaaat accgatgaaa tttccggttc ataccatttc
     3061 gaccgaatgg gtagcatttg ctgaaccata tggtgtagct tgtttttggt tgcctgaacc
     3121 gaaaaaaata atttcagtat ttttagaaaa cctggaaatg tatggaaatc aaaaacttac
     3181 agacacttcg ccatttttcg gcagagaaaa tgcagaaaat caatttgttt gaaacccgga
     3241 atttttttgc cgatatttac tgccttgaac ctggccaggc ccaaaaagcg cacgcccatg
     3301 caggtgcgga caaaatttat gccgtgatgg aaggaaaagt ggtggccctc attggcgagg
     3361 aagaacagga actttcagcc aacgacgctg tcctggcacc tgctgacgtg attcacggta
     3421 ttcggaatgc gtcttcggaa cgtgccgtgg tatttgtctt tatgtcgcca aacccaaacc
     3481 aggcgaaaga aaaccaatga agaatgaaga attgagaatg aagaatgaag aatgaagaat
     3541 gaagaattga
//
LOCUS       DAOQDO010000364         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19501_length_3546_cov_3.393297, whole genome shotgun sequence.
ACCESSION   DAOQDO010000364 DAOQDO010000000
VERSION     DAOQDO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19501_length_3546_cov_3.393297"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3107)
                     /locus_tag="PKZ53_07160"
     CDS             complement(<1..3107)
                     /locus_tag="PKZ53_07160"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HNJ40250.1"
                     /translation="MYHSLRKQTFEIEAASEQLAMALQSSLGDINRRSLVPVIERVLN
                     EVSIPGQTIKISKVKVDLGTIPFQQLEETAAKRLYHELLRALTDAIAEITQNPTPENR
                     TRSKTESLLGQIEHFLVQGTLPFGFTGAGQFSFERQVLHLAETNPQGLVELVKTHSHQ
                     RYVIERLLYQLGETALRKLLFLLEPEHAALILAYMVNLQAVHKIEPVLALSTKQFSHL
                     LWVLVLGYLAQEHGSQFNRKSFVKSVLEGMAESEGLNFLHILITLHFGLQQTEQELSP
                     KTSLPAVIGELIHEFQANIQSMLESAPPQSIPPEAITYFHLELSKKLNTSDEKSAEIK
                     PEHVLDQRHNQDIWQLVESFLVYGTLPFGTRPEPPVSFELLVLKLAETLPQRFVTLIQ
                     THGQHRQILERLVLRLDEAALRRILQLLEPEHAALILAYLLNLREVHRVQPVLPLSDL
                     QFSNLLWLLVLSYLVQERGSQFNRKTFVKTLLEGMSEREGLDFLQILTTLSLGLQQTE
                     KMLSPRSSLPAIIRELVNDFQKNELIQSFAPPTEPALERKIEAGTQRGWFNRYDWMDA
                     VRYFLKYGVLPWSERLRNPNLTLAEVFQSLTRLTAIQIRQTLQVFPGESVAGIRHLVE
                     HLTEAQVVHLIRILLPQLQQADHPLAESLAFFAGQTSDKTTFYSRLLIAVLENQPLDF
                     EQFASDETTSFQQPSTTTKPHHWPPHQLKSQIASWLNAGDVEPDPRFPLVDLFEALFR
                     FHPGQAGHFLEELQRIPGFGEKIAQRCTDDEGVRLVEFFHPEQSATLNLWLRIFTRIP
                     ASIWPLPQAQRVQVFLGQVLALKNGTPLTRAFFVRLLRRLFGFPLSPEIVQFFLDELD
                     ALVSKGKMPAAVAAAFREALTLVNEGDQVSDILPAMFEPATTASVEALQFQIAVQTYL
                     LGEEFPTEFVSASPEADFQPMERDELHHRALVFLEKFPEAAHSAVSRHLSSPEKREYW
                     VKSLPESALVRLVYLFEPVQCHSLIDAAEVLFSAWLEVAPPNSPMLSGRSLFWRFLLE
                     FLAETT"
     gene            complement(3187..>3546)
                     /locus_tag="PKZ53_07165"
     CDS             complement(3187..>3546)
                     /locus_tag="PKZ53_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40251.1"
                     /translation="LRFGRADRRPPPASTSEETQRESPRDPQPFHYSFTITAVLCLPN
                     CGPDSENYQDQVREIIRQNTPSHILDQYCFLKPWQLYRFEALYWAWRQALEQENLRKL
                     IVASNRLTRFLERHTQL"
BASE COUNT          842 a          862 c          983 g          859 t
ORIGIN      
        1 gtggtttcag ccagaaattc aagcagaaac cgccaaaaca atgaccgccc tgataacatt
       61 gggctatttg gcggagcgac ttcgagccag gctgagaaca gaacctcggc ggcatcaatc
      121 aggctgtggc actgcaccgg ctcaaacaaa tagaccagcc ggacgagggc tgactccggg
      181 agcgatttga cccagtattc gcgtttttca ggcgatgaca gatggcgact gacggcggaa
      241 tgtgccgcct cggggaattt ctccaggaac acaagcgccc ggtggtgcag ttcgtcccgt
      301 tccattggct ggaaatccgc ttctggactg gcactgacaa attcagtcgg aaattcctca
      361 ccgagcaaat aggtttgcac cgcaatttga aactggagcg cttcaaccga agccgtggtc
      421 gctggttcaa acatcgcggg cagaatatca ctgacttgat cgccttcatt gaccagagtg
      481 agtgcttcac gaaaagcagc ggctacggct gcaggcattt ttccttttga aaccaacgca
      541 tccagttcat ccagaaagaa ttgaacaatc tcaggcgaga gtggaaatcc aaacaatcgc
      601 ctgagcagcc ggacaaaaaa ggctctggtg agtggcgttc cgttcttcaa ggccaggacc
      661 tgaccaagaa atacctgcac ccgctgggct tgaggcagtg gccagattga ggctggaatc
      721 cgggtgaaga tcctcagcca gagattcagc gttgcggatt gctctgggtg gaagaattca
      781 accagccgaa caccctcgtc atcggtacag cgttgggcga ttttttcgcc aaaccctgga
      841 atccgctgaa gttcctcaag aaaatgaccg gcctgaccgg gatggaatcg aaagagcgct
      901 tcaaataaat caaccagcgg gaatcgtgga tcaggttcga catccccggc attcaaccac
      961 gacgcaatct gggatttaag ttgatgtggt ggccagtggt gtggttttgt cgtggttgac
     1021 ggttgctgaa acgaggtcgt ttcatcactc gcaaactgtt caaaatcaag cggctgattt
     1081 tccagaacgg caatcagcag tcggctgtaa aaggttgttt tatctgaggt ttgcccggca
     1141 aaaaaagcca gtgattccgc cagtggatgg tctgcctgct gaagttgtgg gagcagaatt
     1201 cgaatcaggt gcaccacctg tgcttcagtc aaatgttcga ccagatgccg gattccggct
     1261 accgactcac ctggaaaaac ctgaagggtc tgtctgattt ggattgccgt caatcgcgtg
     1321 agtgattgaa acacctcagc cagggtgaga tttggatttc gcaagcgttc gctccagggc
     1381 aaaactccgt atttgaggaa gtagcgaacc gcatccatcc agtcataccg gttgaaccag
     1441 ccgcgctgag tacccgcctc aatctttctt tccagtgctg gttcagttgg aggtgcaaag
     1501 gactgaatca attcattttt ttgaaaatca ttcaccaact cgcgaatgat ggctgggagc
     1561 gaagacctgg gcgatagcat tttctccgtt tgttggagac caaggctcag ggtggtcaga
     1621 atctggagaa aatcaagtcc ttcgcgttct gacatgcctt caagcagggt tttgacaaag
     1681 gtcttgcggt taaattgcga accgcgttcc tggaccagat atgacaacac cagcaaccag
     1741 agcaggttgg aaaactggag atcactcaat ggcaatactg gctggacccg gtggacttcc
     1801 cgaaggttaa gcaggtaggc cagtatcaga gccgcatgtt cgggttcaag gagctgcaat
     1861 attcgccgga gtgcggcttc atcaaggcgc aggaccagcc gctcaagaat ctgacggtgc
     1921 tgcccgtggg tttgaatcaa ggtcacgaac cgctgaggaa gtgtttccgc cagtttcaag
     1981 accagtagtt caaatgaaac agggggctct ggcctggtgc cgaaggggag cgtcccatac
     2041 accagaaaac tctcaaccag ttgccagata tcctggttat gtctctgatc caaaacatgt
     2101 tctggtttta tttcagccga tttctcatca gacgtgttga gctttttgga taactcaagg
     2161 tgaaagtatg tgattgcttc gggcggaatg gattgcgggg gagcactctc aagcatgctc
     2221 tgaatattcg cctgaaactc gtggatgagt tcgccaatca cggctggcag cgacgttttt
     2281 ggcgaaagct cttgttcggt ttgctgcagc ccaaaatgga gcgtgatcag aatatggaga
     2341 aagttgagcc cttcgctttc ggccatgcct tcaagcacgg atttgacaaa gcttttgcgg
     2401 ttaaactgtg aaccgtgttc ctgggccaga tacccaagta ccagcaccca caggagatgc
     2461 gaaaattgct ttgtgctcag cgccagcacc ggttcgattt tgtggacggc ctgaaggttc
     2521 accatatagg ccagaatcag cgccgcgtgt tctggctcca gcaaaaagag cagttttcgc
     2581 agcgcagttt caccgagctg gtacagcagc cgttcgatga cataccgctg atggctatga
     2641 gttttgacca gttcgaccag tccttgaggg ttggtttcag ccaggtggag cacctgtcgt
     2701 tcaaatgaaa attgacctgc cccggtgaac ccaaacggga gcgttccttg aaccaggaag
     2761 tgttcaatct ggcccagcag gctttcggtt ttagatcgag ttcgattttc cggtgtcgga
     2821 ttttgggtga tttctgcgat ggcatctgtc agggcccgca ggagttcgtg atacagccgt
     2881 ttggctgccg tttcttcaag ctgttgaaat ggaatcgtgc ccagatcaac cttgaccttc
     2941 gaaatcttga tggtctggcc tgggattgaa acttcgttaa ggacgcgttc aatgaccggc
     3001 accagcgaac gccgattgat atcacccagg ctggattgga gcgccatcgc cagttgttca
     3061 ctggcagcct cgatttcaaa ggtttgtttg cgaagtgaat ggtacatttg gaatgcggcg
     3121 gagtgcggtg gcttgacacc gctttggtat tttggagtgc ggcagcttga cgccgctttc
     3181 tcgcttttaa agttgtgtat gcctttctaa aaaccgcgtc agtcggtttg aggcaacaat
     3241 cagttttcgc aggttttctt gttcgagtgc ctgtcgccac gcccagtaca atgcttcaaa
     3301 ccgatacagt tgccagggtt tcaggaagca atattgatcc agaatatgcg acggcgtgtt
     3361 ttgacgaatg atttcacgga cttgatcctg atagttctca gaatctggcc cgcaattcgg
     3421 aaggcacagc accgccgtga tcgtgaaact atagtgaaat ggctgcgggt ctcgcgggct
     3481 ttcacgttgg gtttcttcgc tggttgaagc tgggggcggt cttcggtcgg cgcgaccaaa
     3541 gcgcag
//
LOCUS       DAOQDO010000365         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19534_length_3544_cov_2.840069, whole genome shotgun sequence.
ACCESSION   DAOQDO010000365 DAOQDO010000000
VERSION     DAOQDO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19534_length_3544_cov_2.840069"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKZ53_07170"
     CDS             <1..1057
                     /locus_tag="PKZ53_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNJ40252.1"
                     /translation="RHFLFVLIGLWGIFIIFTLFELINHIAQNKIKAGVVVEYLLFLT
                     PQVINYMLPFAVLVAVLVTFGLLGRTSQLIALNTSGQSVYRLTVPVLIGALLIGATML
                     ASQEYVLPFTNRTQDYLRNLIRGKQLPPQTFYQTNRKWFRGQNNRIFNFRHFDTNKNE
                     FVGLSIYETNPETASLVSRIYVKAALWDPKTTEWILTKGWKRTFTPDGRPEGRTEITE
                     ARLKLPEDPEYFTQGIPESSKMSITELQEQIIELKRIGADVMSLNIELQSKFAACLAC
                     LVMALVGLPFSFTIGKRGALVGVSIGIAIAIIFWGTIGVFDQLGRYELLPPFWAAWGP
                     NLLFSVGGVYLLLTAKT"
     gene            1154..2245
                     /gene="wecB"
                     /locus_tag="PKZ53_07175"
     CDS             1154..2245
                     /gene="wecB"
                     /locus_tag="PKZ53_07175"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNJ40253.1"
                     /translation="MMKILSVVGARPNFMKIAPILNAFRRHPEIESKLVHTGQHYDAE
                     MSDAFFRDLQIPAPDINLGIGSGSHAEQTARVMLAFEPVLLQEKPDWVVVVGDVNSTM
                     ACTLVAVKLGIKVAHVEAGLRSFDRQMPEEINRLVTDALADLLLTPSPDGDENLLREG
                     VHPARIRRVGNVMIDTLMMSLEQAKQSSIVDQMELTPGNFAVVTLHRPSNVDAVETLT
                     GILGSLGRLQAELPVIFPVHPRTRARLTEFGLTSQLAAWPNFRLCDPLGYLDFLRLWS
                     QSRLVLTDSGGLQEETTALGIPCLTLRENTERPITVTEGTNRIVGTNPDTIDAAIRDV
                     LHGAVFPKRIPELWDGKTAERILACLLQG"
     gene            2396..3094
                     /locus_tag="PKZ53_07180"
     CDS             2396..3094
                     /locus_tag="PKZ53_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40254.1"
                     /translation="MHNWFLSSQSKKQNSFFRLDEPGLAQLRQGWAELTRPLSARPER
                     LQTLWEHLVKNYSEPHRSYHNLSHIKALFDHIQPIRDHLEDIEVVEWAIWFHDVIYRT
                     RHSDNEEQSAAFAFEWLNELEMPPLRRDMVRRLILATRQHSVVHLPPDGAYFLDADLA
                     ILGAPEPVYLEYRQAIRREYSWVPEFVYRRKRRAVLESFLTREQLYFTSEMRARLEAQ
                     ARQNLSAEIQFLSS"
     gene            3105..>3544
                     /locus_tag="PKZ53_07185"
     CDS             3105..>3544
                     /locus_tag="PKZ53_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNJ40255.1"
                     /translation="MSVHQAAKEKKVTIQTLLDRKRNHEKITSLTAYDFPMARLVDEA
                     GADFILVGDSLANVVLGYETTLEVTLDDVIFCTRAVRRGVKRALVVGDMPFGSYHTSV
                     ERALDSAVRLIKEGGAEAVKLEGGQKRAYLIEKIVDNEIPVMGH"
BASE COUNT          844 a          848 c          914 g          938 t
ORIGIN      
        1 ccgccacttc ctgtttgtgc tgattgggct gtggggcatc tttatcatct tcaccctgtt
       61 tgaactgatt aaccatattg cccagaataa gataaaagct ggtgttgttg ttgagtacct
      121 gttgtttctc acgcctcagg tcattaatta catgctccct tttgccgtcc tggtcgcggt
      181 gctggtgact tttggattgc ttgggcggac atcacagtta attgccctca acaccagcgg
      241 acagagcgtg taccggctga cagtaccggt tttgattggt gccttattga ttggggcgac
      301 catgctggca tcccaggagt atgtattgcc gtttaccaat cgcacccagg attacctccg
      361 caatttgatt cgcggcaaac aacttccgcc tcagaccttt taccagacca accgcaagtg
      421 gtttcgcggc caaaataacc ggatttttaa ctttcgccat tttgacacca acaaaaatga
      481 gtttgttggc ctgtcaattt atgaaaccaa tcccgagacc gccagtctgg taagccggat
      541 ctatgtcaag gcagcgctat gggacccaaa aaccacggaa tggattttga ccaaaggctg
      601 gaaacgaact tttactcccg atggtcgccc tgaaggacgg actgaaataa ccgaggcccg
      661 gcttaaactt ccagaagatc ctgagtactt tacccaggga attccggaat caagcaaaat
      721 gagcattacc gagttacaag agcaaattat tgaattaaaa aggattggcg ccgatgtgat
      781 gagtttgaac atcgagttgc aatcgaaatt tgctgcctgt ctggcttgcc tggtcatggc
      841 gctggttgga ttgccgtttt cattcacgat tggcaaacga ggggcgctgg ttggggtcag
      901 tatcggaatc gcgattgcca ttatcttttg gggaacgatt ggggtttttg atcagttggg
      961 acgatatgaa ttgctcccgc cattttgggc tgcctggggg ccgaacttat tattcagtgt
     1021 cggcggtgtg tatctgctgc taactgcaaa aacctgaccc cggaaaataa agaaggtaga
     1081 atgtagaaat tcagcggctc aaagcttttt cttcatgctc aattcttcat tcttcatttc
     1141 ttgaaggaaa ttattgatga aaattctcag tgtcgtgggc gcgcgcccaa acttcatgaa
     1201 aattgctccg atcttgaacg cctttcgtcg tcatccagag attgagagca aactggttca
     1261 tactggtcag cactatgacg ccgaaatgtc agatgccttc tttcgggatc tgcaaattcc
     1321 agccccagac atcaatttag ggattggttc cgggtcacat gccgaacaaa ctgcccgggt
     1381 gatgctggct tttgagccgg tgcttctgca ggaaaaacca gattgggtgg tggtggttgg
     1441 tgatgtgaac tcaacaatgg cctgcacgct ggtggcggtt aagcttggca tcaaggtggc
     1501 ccacgtcgaa gccggtctcc gcagtttcga ccgtcaaatg cctgaagaaa tcaaccggtt
     1561 agtgactgat gcactggctg atttgctcct gacgcccagc ccagacggag atgaaaacct
     1621 gctgcgcgaa ggtgttcacc ccgccaggat tcgtcgagtg ggaaatgtaa tgattgacac
     1681 cttgatgatg agtcttgaac aggcaaaaca atcgtcaatt gttgaccaga tggaactgac
     1741 acctgggaat tttgccgtgg tgacgctcca tcgtccgtca aatgtggatg ctgttgagac
     1801 gctgactgga attttaggaa gcctgggccg acttcaggca gaattaccgg tcattttccc
     1861 ggttcatccc cggacccgtg ccaggttgac ggaatttgga ttgaccagcc agcttgcggc
     1921 ctggccaaac ttccggttat gtgatccatt gggctatctg gatttcctgc gactgtggag
     1981 ccagtcacgg ctggttttga ctgattcagg tgggctgcaa gaagaaacca ccgcgcttgg
     2041 catcccctgc ctgaccttgc gtgaaaatac tgaacgcccg attacggtca ctgaaggcac
     2101 aaatcgtatt gttggaacga atcctgatac cattgatgct gcaatccggg atgttttgca
     2161 tggcgcggtt tttccaaagc gcatccctga actctgggat ggaaaaaccg ctgagcgtat
     2221 tctggcctgt ttactccagg gatagagctt gatcatcaac ccggcagttt ttagcctgaa
     2281 catagaactg gctgtcgggt ttgactcggt gttttctctt cgtatccgct attctgtacc
     2341 agccctggaa ctccatcagt ttcaggtttg atttctggat ctgtggcgga attcaatgca
     2401 caactggttt ctttcatctc aatcaaaaaa gcaaaactca ttttttcgac ttgatgaacc
     2461 tggtttggcc caactcaggc agggatgggc tgaactgacg cgtccgttat cggccaggcc
     2521 agaacgtctc cagactctct gggaacacct ggttaaaaac tattccgagc cgcatcgttc
     2581 ctaccataac ctcagccata tcaaagcttt atttgaccat atccagccga ttcgtgacca
     2641 cctggaagat atcgaagtgg tggagtgggc gatctggttt catgatgtca tttaccgcac
     2701 tcgccacagt gataatgagg aacagagtgc agcatttgct tttgagtggt tgaatgaatt
     2761 agaaatgccc ccacttcgcc gcgatatggt ccgacgcctg attctggcaa ccaggcaaca
     2821 ttcagtggtc catcttccac ctgacggcgc ttattttctg gatgccgacc tggcaatttt
     2881 aggcgccccc gaaccggttt atcttgaata tcgacaggcc attcgccgtg aatattcgtg
     2941 ggtgccagag tttgtgtatc gccgtaagcg acgggcagtt ctcgaaagct ttttgacaag
     3001 ggagcaattg tactttacaa gtgaaatgcg cgcccgactg gaagcccagg cacgtcaaaa
     3061 cctttcagcc gaaatccaat tcttatcgag ttagtctttt tgttatgagt gttcaccaag
     3121 ctgcaaaaga gaaaaaagtg acgattcaga cgcttctgga tcgcaaacga aaccacgaga
     3181 aaatcacttc gctcaccgcc tatgattttc caatggcccg gctggttgat gaagccgggg
     3241 ccgattttat tctggtcgga gattcgctgg ccaatgtcgt gcttgggtac gaaactaccc
     3301 tggaagtgac gctcgacgat gtcattttct gtacccgggc agttcgacga ggcgtgaagc
     3361 gggcgctggt ggttggggat atgccgtttg gaagttatca caccagcgtg gaacgggcgc
     3421 tcgattcagc cgtccggttg atcaaagaag gtggtgccga agctgtcaaa ctcgaaggcg
     3481 gtcagaaacg ggcatatctc attgagaaaa ttgtagataa tgaaattccc gtgatgggac
     3541 atat
//
LOCUS       DAOQDO010000366         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19573_length_3539_cov_2.886912, whole genome shotgun sequence.
ACCESSION   DAOQDO010000366 DAOQDO010000000
VERSION     DAOQDO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19573_length_3539_cov_2.886912"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKZ53_07190"
     CDS             <1..583
                     /locus_tag="PKZ53_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNJ40256.1"
                     /translation="AVIELIALWHQCLEERWVHPLIAMAAFNLDFLCIHPFRDGNGRV
                     SRLLFLLQCYQLGYEVGRYISLERIIEQNKERYYETLEASSQQWHQGHHDPWPVIQFF
                     LFVLKTAYREFEHRFEQASAPKGSKTELIELVIRAVSDTFTITDLERAAPGVSRDMIR
                     AVLKRLKNDGQVVCRGRGPGARWSAVSQKQPKE"
     gene            complement(720..1748)
                     /locus_tag="PKZ53_07195"
     CDS             complement(720..1748)
                     /locus_tag="PKZ53_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0019992 - diacylglycerol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 domain-containing protein"
                     /protein_id="HNJ40257.1"
                     /translation="MNTPHRFEIKQELLPTRCDVCHQADLFDPKTGICERCHGVLPSE
                     PDPQSGLLVYQRPIGLNWRITEIMTNAPPLPFRSLAQALVRAPRLLGPVFVPVVCVEM
                     VVRWLTIKCIDFPLTSLMGNELSLIVILGLILVCLVLSPLQTAALTLAVANTQQDEPD
                     QLPTIYKQIWERRSSLISALIVMGSYRLLIGIWGMLFLLPLGIQLDFPVFPSVFLCVS
                     LLSLIPPLYAFGSSSFVMEFLLLDRFNQDPLISSQTYFGRNWRMLSVYWLIRWVTIFG
                     TASVLAVLLYQLGYAFIWFWMVMFPLHSLLTAFFTITKTLLFTEIESDVRRRLNEALP
                     VTPNPSEQ"
     gene            2025..2483
                     /locus_tag="PKZ53_07200"
     CDS             2025..2483
                     /locus_tag="PKZ53_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNJ40258.1"
                     /translation="MSSLFYRIQSPHVIREQFDDEVVIVNLESGAYYSLDDVSAVIWT
                     GLEHKKSVSEILTDMCATYTGELAEIETELGRFLQTLCTENLIKPEPDPSQSASAPPI
                     QTRKRSDTSRPFSAPRLNKYTDMQDLLLLDPIHEVDETGWPTPKTLPTAD"
     gene            2447..3535
                     /locus_tag="PKZ53_07205"
     CDS             2447..3535
                     /locus_tag="PKZ53_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HNJ40259.1"
                     /translation="MADPKNSTHSRLTAAPTALITTYYQANSEAFEQARHQSEAVTDR
                     FYTIAGFTIRLRFAGPALLPRLLPALEHLATDPVSFPDLTVCLWDSGSTSVPAPSPPW
                     EAGRYFAHGEVKEFCTDRFYTHLNVGPNILSLLDFDRKEAIYWAPSLKVIPNYEVSVP
                     LRTILHTWLSRFGRHMVHAGAVGLPTGGVLLIGHGGAGKSNTALSCLSSELKYLSDDH
                     CLVAAEPEPTVYSVYGTGKLFPQDIARIPALASLGDDAPIVQTEKALFYLHRHFAHKI
                     MTEFPLKALLLLQVTGKVDSCLVPVSGAMGLRALAPGQMMQWPTAGPATFQTIAHLCR
                     QLPCYRFELGTDRAQIPAMILKLLKDQG"
BASE COUNT          919 a          869 c          886 g          865 t
ORIGIN      
        1 ggcggttatc gaattgattg ctctgtggca ccaatgtttg gaagagcgat gggttcatcc
       61 actgattgcg atggccgcgt tcaatctgga ttttctgtgc attcatccat ttcgagatgg
      121 caatggacgt gtttccaggt tgttgtttct gctccaatgt tatcagcttg ggtatgaggt
      181 tggacgatac attagcctgg aacggatcat tgagcaaaac aaagagcgat attatgaaac
      241 cctggaagcc agttcacagc agtggcacca gggtcaccac gatccctggc cggtgattca
      301 gttctttcta tttgttctca aaacggcgta ccgggaattt gagcatcggt ttgaacaggc
      361 gtcagccccc aaaggatcaa aaaccgaact gattgagttg gtcattcgag ctgtttccga
      421 cacttttacg attactgatt tggaacgggc agcccctggt gtgagtcggg acatgattcg
      481 tgccgttctc aaacggctca aaaatgatgg acaggttgta tgtcgtggtc gtggtcctgg
      541 tgccagatgg tctgctgttt cccaaaaaca gcccaaagaa taagggtaat acccttaaaa
      601 gagggtaaga aagagggtaa tacgacttgg ggctgagggt atcgggctaa gggttgaggg
      661 aaatccaggt ttttcaataa tttagcctcc ctataacacg atgccttcat ggcacaaggc
      721 tattgctctg agggatttgg ggtcactgga agtgcttcgt tgagacgacg ccggacatca
      781 gattcgattt cagtaaacag caaggttttc gtgatcgtga aaaaggcggt cagcaggctg
      841 tgcagcggaa acatcaccat ccaaaaccag ataaacgcat agcccagttg gtacaaaaga
      901 acggcgagca cactggcagt cccaaagatg gtgacccatc gaatgagcca gtacaccgag
      961 agcatccgcc agtttctccc aaagtaagtc tgactggaga tcaagggatc ctgattaaag
     1021 cggtcaagga gcagaaattc catgacaaag ctgctgctgc caaacgcata cagtggcggt
     1081 atcaatgaga gaagggacac acacaggaaa acagatggga atacagggaa gtcaagctga
     1141 ataccaagtg gaagcaggaa aagcataccc caaattccaa tcaatagccg atatgacccc
     1201 atcacgatca atgcactgat gagtgaagac cttcgttccc atatctgttt gtaaattgtt
     1261 gggagttgat ctggctcatc ttgttgggtg ttggcaacgg caagtgtcaa ggcagcagtt
     1321 tggagtggac tcaataccag gcagacaaga atcagcccca ggatcacaat caggctcaac
     1381 tcgtttccca tcagagatgt aagtgggaaa tcaatacatt tgattgtcag ccatcgaaca
     1441 accatttcca cacacaccac aggaacgaag accggtccaa gcagccgtgg tgccctgacc
     1501 aacgcctggg ccaacgaccg aaagggcagc ggcggggcat tcgtcataat ttctgtaatg
     1561 cgccaattca aaccaatagg gcgttgatag acgagcaaac cactttgggg atccggttca
     1621 gatggtaaaa cgccgtggca ccgctcacaa ataccagtct tcgggtcaaa caaatcagcc
     1681 tggtggcaca catcacagcg tgttgggagc agttcctgct tgatttcaaa gcggtgtgga
     1741 gtgttcattg atttgccttt atggaaagaa taaatcgccg tacttataga atggtcaatg
     1801 ggttgctaag tttagatttc tgatggggtc aaaaaatcaa aataattgga gaagtgggat
     1861 tttctcagtt caagctcgtt ttcttcagcc ctcagcccca agccctcagc ccaaccagag
     1921 atgaaaccag ttgtcagtga gactttggat gcatatagtc tgtttcctga gttctggtcc
     1981 atcttcaaaa ccttcagttc taaactcttg tcccggagtc ctttatgtct tccttgtttt
     2041 atagaatcca atcaccgcac gtcattcgcg agcaatttga cgatgaagtg gtgattgtca
     2101 acctcgaaag cggtgcttat tacagccttg atgatgtgag tgcggtcatc tggactggcc
     2161 tggaacacaa aaaatctgtt tccgaaatcc tgaccgacat gtgtgccacc tatactggtg
     2221 aactggctga gatagaaaca gaactgggtc ggtttctcca aaccctgtgc actgaaaatt
     2281 taatcaagcc tgaacccgac ccgtctcaat cagcttcagc acctccaatc caaaccagga
     2341 aacgctcaga cacttctcgc ccgtttagtg cccccaggct caataaatac actgatatgc
     2401 aggacttgct tctgcttgat cccattcacg aagtggacga aaccggatgg ccgaccccaa
     2461 aaactctacc cacagccgat taactgccgc acccaccgca ctgattacaa cctactatca
     2521 agcaaactct gaggcgtttg agcaggccag acaccagagc gaagcggtga ctgaccgatt
     2581 ttataccatc gctgggttta ccatccggct tcggtttgca ggcccagcct tgctgccacg
     2641 gctgcttcca gcgttagagc atctggctac cgatcccgtc tcgtttcctg atctcaccgt
     2701 atgtttatgg gatagtggtt ccacttcggt accagcacca tccccaccct gggaagccgg
     2761 gcgatatttt gcccacggtg aagtgaaaga attctgtact gatcggtttt acacccattt
     2821 gaatgtcggc ccaaacatcc tgagtttact cgactttgac cgcaaagagg ccatttattg
     2881 ggccccaagt ttgaaggtaa ttccaaatta cgaagtctct gtcccgctcc ggaccattct
     2941 gcatacctgg ctcagtcggt ttggccgcca catggttcac gccggagcgg ttggattgcc
     3001 aacgggtggg gtgctgctca ttggtcatgg cggcgctggg aaatcaaaca cggcgctgtc
     3061 gtgtctttct tctgaattga aatatttgag cgacgatcat tgtctggtcg cggcagaacc
     3121 ggaaccaacc gtgtatagcg tctatggcac tggaaagttg tttccacagg atattgcccg
     3181 tatcccggcg ctggcctcac ttggtgatga cgctccgatt gtgcagaccg aaaaagcact
     3241 gttttatctg caccggcatt tcgcacacaa gatcatgaca gaatttccat taaaagcgct
     3301 gttactgcta caggttaccg ggaaggttga ttcctgtctg gtgccagtct cgggtgcgat
     3361 gggactgcgt gccttggctc caggacaaat gatgcagtgg ccaacggctg gaccagccac
     3421 atttcaaacc attgcgcacc tgtgtcgtca acttccttgt tatcggtttg aactcggcac
     3481 cgaccgggcg caaattccag ccatgatttt gaaattgctg aaagatcagg gctgaagaa
//
LOCUS       DAOQDO010000367         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19616_length_3535_cov_3.233046, whole genome shotgun sequence.
ACCESSION   DAOQDO010000367 DAOQDO010000000
VERSION     DAOQDO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19616_length_3535_cov_3.233046"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..532
                     /gene="rpsH"
                     /locus_tag="PKZ53_07210"
     CDS             140..532
                     /gene="rpsH"
                     /locus_tag="PKZ53_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNJ40260.1"
                     /translation="MTDPIADMLTRLRNALSARHQKVDVPASKMKLEIARILKEEGYI
                     NNYKVAGESPKRMIRLYLRYGPKGEPVINVLQRVSRPGCRRYISSTEIPNVLGGLGIN
                     IVSTSHGIMTGKQARKSKVGGELVCQIY"
     gene            603..1172
                     /gene="rplF"
                     /locus_tag="PKZ53_07215"
     CDS             603..1172
                     /gene="rplF"
                     /locus_tag="PKZ53_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNJ40261.1"
                     /translation="MSRVGKKPIPLPSGVSVNITPEKIDVKGPKGALETKVPSGISLR
                     QDGATLVIERTSDEFAALHGLVRALVANAVKGVTDGFVQQMDVVGVGYKAELKGRMIA
                     FSLGYSHPVEFTLPQGIDCKIEKLPRQIPQYQTTVTLTGIDKHQLGQVAADLHSLRKP
                     DAYKGKGIRYTNRVTRLKPGKTGGKGGKK"
     gene            1306..1665
                     /gene="rplR"
                     /locus_tag="PKZ53_07220"
     CDS             1306..1665
                     /gene="rplR"
                     /locus_tag="PKZ53_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNJ40262.1"
                     /translation="MALKERAEVRKAIHRRIRKKVFGTPERPRLCVFRSLKHITVQII
                     DDSKGETLCSASTVEKTLREQSGGNIQAAEAIGKLIATRAKEKGIDSVVFDRGGYIYH
                     GRVRHLAEAAREAGLKF"
     gene            1683..2177
                     /gene="rpsE"
                     /locus_tag="PKZ53_07225"
     CDS             1683..2177
                     /gene="rpsE"
                     /locus_tag="PKZ53_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNJ40263.1"
                     /translation="MQRINPDGLDLKDNVISINRVTKVVKGGKNMSFSVLVVVGDNNG
                     IVGYGSGKAKEVPIAIRKAVESAKKNLVRIPLEGHTLTHPMIGNYGAGKVMLKPAPEG
                     TGVIAGAAVRSIMQALGVQNVVTKVIGSNNPHNIVRATFDGLGRMKSRDDIARIRGKR
                     PEEL"
     gene            2181..2390
                     /gene="rpmD"
                     /locus_tag="PKZ53_07230"
     CDS             2181..2390
                     /gene="rpmD"
                     /locus_tag="PKZ53_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNJ40264.1"
                     /translation="MSEINSGKGTTARIKIQWYRSFISAPKTHKTIVRSLGFTKLQQI
                     VERPDTPSTRGAVAKVPHLLRIVEG"
     gene            2594..3040
                     /gene="rplO"
                     /locus_tag="PKZ53_07235"
     CDS             2594..3040
                     /gene="rplO"
                     /locus_tag="PKZ53_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNJ40265.1"
                     /translation="MGLHNLEAPKGANKRKKRVGRGPGSGHGKTSTRGHKGQKSRSGY
                     SSMRGFEGGQMPLHRRLPKRGFTNIFKKEWTEINLKMLELLYEANDTVTPDSLVEKGV
                     VSKLAKDGVVILANGELTKPLKVQAHRFSDKAKEKIQAAGGTVEVC"
     gene            3335..>3535
                     /locus_tag="PKZ53_07240"
     CDS             3335..>3535
                     /locus_tag="PKZ53_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNJ40266.1"
                     /translation="MFDQIVKSFRNIFSVPELRNRIFYTLGMLIVYRIGSHIRTPGID
                     PERLSALWRDLQGSLVGVLDLFS"
BASE COUNT          966 a          830 c          946 g          793 t
ORIGIN      
        1 gaccaaatcg agctggtaaa gtgagttgtg gtaagtgtcc ctggttcggc gccgatgggc
       61 ctgagctgta gggggaggcc gggtgggtgc cgcccaagcc tgaatttttc tgacaaatat
      121 aaggttgtgg agtgattcga tgactgatcc aatcgcagac atgctgacac ggttgcgcaa
      181 tgcgctcagt gcccgacatc aaaaagttga tgtcccggct tcaaaaatga agcttgagat
      241 tgcgcgaatc ttaaaagaag aaggttatat caataattac aaggtggctg gtgaaagccc
      301 caagcggatg attcgcctct acctgcggta tggtcccaaa ggtgaaccgg tgatcaacgt
      361 gttgcagcgc gtttcgcgac ctgggtgccg acgctatatt tccagcactg aaatcccaaa
      421 tgtcttgggt ggcctgggaa tcaatattgt cagcacctcg cacgggatta tgaccggaaa
      481 acaagcccgg aaatccaaag ttggcggcga actggtctgt caaatttact aattcagcgt
      541 tggccgatct gatgggcaag aactgagcaa tgatcagcca gaagaggtag tgagaaaagc
      601 atatgtcacg agttggaaag aagccaattc ctctgccttc gggcgtttcg gtgaatatca
      661 cacctgaaaa aatcgatgtc aaaggaccca aaggcgcact tgagaccaag gtgccatcgg
      721 gaatcagcct gcgacaagac ggggcaaccc tggtgattga acggaccagc gatgaatttg
      781 cagccttaca cggtctggtt cgcgccctgg tggccaacgc cgtcaaaggt gtgacggacg
      841 ggtttgttca gcaaatggat gtggttggcg tcggttataa ggccgaatta aagggtcgga
      901 tgatcgcctt cagccttgga tattcacacc cagtggagtt tacccttccc caaggaatcg
      961 attgcaaaat tgaaaaactc ccccgacaaa tcccccagta ccagacaacc gtcactttaa
     1021 ccgggattga taagcaccag ttgggacagg tcgctgctga tcttcactcc cttcgcaagc
     1081 cagatgctta taaagggaag ggaattcgtt acacaaatcg tgtgacacgc ctcaagcctg
     1141 gaaagactgg cggcaagggc ggcaagaagt aacagtccgc gtcgtcacgg tcaaccgcaa
     1201 cgtcgcagtg aaatctgtgc cggcaggagt ctggatcagt ttccctcctg cctggcgacc
     1261 cagactggca cattgatcag gttacaagta taagaggagt gaactatggc attaaaagag
     1321 cgtgcagagg tgcggaaggc aatccaccgg cgtattcgca agaaagtgtt cggaactccc
     1381 gaacgcccac gcctgtgcgt gtttcgcagc ttgaagcaca tcactgtgca aatcattgat
     1441 gattcaaaag gcgaaacatt gtgctcggct tcaacggttg aaaaaacgtt gcgtgaacaa
     1501 tcaggtggca atatccaggc ggcagaagcc attgggaaat tgattgcaac ccgagcgaaa
     1561 gaaaaaggga ttgactctgt ggtgtttgat cggggcgggt acatctacca tggccgcgtc
     1621 cgccacctgg ctgaagccgc ccgtgaagcc ggtctcaagt tttaataaat ggggagcgta
     1681 cgatgcaacg aatcaacccg gatggactcg atttaaagga taatgtaatt tcgattaacc
     1741 gcgtgaccaa agtggtcaaa ggcggtaaaa acatgagctt cagcgtgctg gtagtggtcg
     1801 gtgacaacaa tggaatcgtt ggctatggaa gcggtaaagc caaagaagta cccattgcga
     1861 ttcgcaaggc agttgaatca gccaaaaaga acctggttcg cattccactg gaaggtcaca
     1921 cgttgacaca cccgatgatt gggaactatg gagctggaaa agtcatgctc aaaccggcac
     1981 ccgaaggaac tggcgttatt gctggtgcgg ctgtccggtc aattatgcag gctttaggcg
     2041 tccagaacgt agtcaccaaa gtcattggct ccaacaaccc ccataacatc gtgcgggcta
     2101 cctttgatgg cctgggcagg atgaaaagcc gtgatgatat tgcccgcatt cgtgggaagc
     2161 ggccagagga gttataagcc atgagtgaga taaacagcgg aaaagggaca actgcccgaa
     2221 tcaaaattca atggtaccgg agctttattt cggctcccaa aacccataaa accattgtgc
     2281 gcagcctggg atttaccaaa ctgcagcaaa ttgtcgagcg tccagatacc ccaagcaccc
     2341 gtggggctgt ggcaaaagta ccgcacttgt tgcggatcgt ggaaggataa gtctcaccac
     2401 tgagtcactc caccggattg agcctggtga tgttggtcga agcaacacac aacaagctca
     2461 gaccatgaat aactgctgaa aaaattgtaa ggaggttgca gctccccgcc gaaaagaccg
     2521 gttgattcat tcctgtgaat gacgttgata cggaaaaagt atcggatcaa gctgcaagga
     2581 gacttcgatg gcgattggat tgcacaacct ggaagcccca aagggggcaa ataagcggaa
     2641 gaagcgtgtt ggtcgtggac ctggttcagg tcacggaaaa acatcaactc gcggccataa
     2701 agggcaaaaa tcacgttcgg gatattcctc aatgcgtggt tttgaaggtg gtcagatgcc
     2761 attgcaccgc cgactgccaa agcgtggatt taccaacatc ttcaaaaaag agtggactga
     2821 aatcaatctc aagatgctgg aactcttgta tgaagccaac gatacggtga cgcccgatag
     2881 cctggttgaa aaaggtgttg tcagtaagct ggcaaaagac ggagtcgtga ttttggccaa
     2941 tggcgagttg actaaaccct tgaaagtaca ggcccaccgg tttagcgaca aagcgaaaga
     3001 aaaaattcaa gctgcaggtg gcacggttga agtgtgctaa tcggctttgc gtttcctgat
     3061 ctctttcctt ccagaggatt gatatggcca ggaaacgctt cgctttattt actggttggt
     3121 attaaatttg catattcacc aaagaaactc ctcagaagcg gaatttttcg acagaaaaac
     3181 cccctgcctc tgcacattga gccttcatgg agtttgatca tccacgcgct tcacattttt
     3241 gagaagccat tccacgccga agaagttaaa tctagatccc atcacctgat ggaccgtcta
     3301 gattttccgg attccacacc tctcctgagg atgtatgttt gaccagatcg taaagagctt
     3361 tcgtaacatt ttttctgttc ctgaactgcg gaatcgcatt ttctataccc tgggaatgct
     3421 gattgtctac cgcattggca gccacattcg aacccctggc attgacccag agcggttaag
     3481 tgcgctgtgg cgcgacttac aaggctccct ggttggggtg cttgaccttt tttca
//
LOCUS       DAOQDO010000368         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19617_length_3535_cov_3.225000, whole genome shotgun sequence.
ACCESSION   DAOQDO010000368 DAOQDO010000000
VERSION     DAOQDO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19617_length_3535_cov_3.225000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="PKZ53_07245"
     CDS             <1..1480
                     /locus_tag="PKZ53_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40267.1"
                     /translation="LWKISIWLLALTGWFNLATVSVIAQRTSSPKNGKKVESTRNAAS
                     EDTALGESLDDRQALAAQKFDTGQDFHAQGKLTDAIKLYDEALQLDDQLFAVHYQRGI
                     ALLSLNRFEDAAAALTRCVELQDDFARGFAMLGEAQLRCKKPVEAEKAFLRAIELDSS
                     LGFARFGAAQLMLARKAPTEALTLLKELAQTPPEDPVFYAVYAEAQRLSGDAPGAFQT
                     LSDGLTRFGNQPQLLRGRAMAYLNQKNYPKALADFQAVYTIEPDPVLARDMLDLYRAA
                     KLTEDGIQFAQVAFKQYSSNGPLRAAFGELLMEGNRTTEAIEQYSELTKQEPKNATWW
                     VKLGDAAQATDPAVALNAYQKALSINPRSPDAQAGVASGLLKSQRFQEAASSFRTILN
                     QDPESYAAHAGLATALFKLEQFPTAAQHFLWMIQKRPDVPVTYYFLGICFDRLGDYAQ
                     ALKAYELFVERANPQTNQLEIEKVNLRLPSLKRQVEKQGPRK"
     gene            1751..2332
                     /locus_tag="PKZ53_07250"
     CDS             1751..2332
                     /locus_tag="PKZ53_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40268.1"
                     /translation="MRHHQTQHLVLGKVWLVWGLFFLVIGIQTPPCLASTTGTVETGM
                     EIPAVLTEAERSVLVEENDPEDHIEAGLKIGLARLNQAVQAMQTEEFDNVHGSLAAYN
                     QLLSYLECYARQSPMKAKKRDKLFRLMEVKLRHQLPLLEAIARTCPFDEDNNSPEMTL
                     SRVQGLRKQLLNVVFGGDFFKPCESTAINAFKE"
     gene            complement(2494..>3535)
                     /locus_tag="PKZ53_07255"
     CDS             complement(2494..>3535)
                     /locus_tag="PKZ53_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40269.1"
                     /translation="IRQNAIEANQSKRRIFTFGVGYDVNSPLLDGLAPDSRAVTTFVQ
                     PNEDVEVKVSELFKRLGNPLMVNPKLEIRSARGEVTSRGAREMFPSELNDIYENDQLV
                     VLGKYQTNAPLTFHVSGNYLGVPQSFSFTFGFDRASQQNSFIPRLWASRKIASLIQEI
                     TKAGADPQSAAKDPRLKELTDEIVRLSMEFGILTEYTAFLATETTDLTRRDEVLNRVQ
                     NELAHNAQQTRTGVGGMTQTGNNAAQRTQTTLNYSNAYVTQQNERTQIQNVRQIQDQT
                     LFRRSNGWVDARLVQNKNLKADQIIEFGTPEFNGLLETLSREGRQGVLAEPGNVFLSV
                     NGKVVQVNMPRP"
BASE COUNT          853 a          956 c          875 g          851 t
ORIGIN      
        1 cttgtggaaa atttccatct ggctgttggc gctgacaggg tggttcaatc tggccaccgt
       61 ttcagtgatt gctcaacgta cgtcctcccc caaaaacgga aaaaaggtcg aatcaacccg
      121 caatgcggct tcggaagata ccgcattggg cgaatcgctc gatgaccggc aggcactggc
      181 agctcaaaaa tttgacacag gtcaggattt tcatgcccag ggcaagttaa ccgatgccat
      241 caagctctat gacgaagccc tgcaactgga tgatcagttg tttgccgttc attaccagcg
      301 cggcatcgcc ctgctctcac tcaaccggtt tgaagacgcc gcagccgcat tgacccgctg
      361 tgtcgaactt caagatgatt ttgcccgtgg atttgcgatg cttggtgaag cccaactccg
      421 ctgcaaaaag ccggtcgaag ctgaaaaagc gtttctgcga gcgattgaac ttgattcttc
      481 ccttggattt gcccgctttg gcgctgccca actgatgctg gcccggaaag ctccaaccga
      541 agcactcacc ctgctcaaag agcttgccca gacaccgcct gaagatcctg tattttatgc
      601 ggtctatgcc gaagcccagc gactcagcgg cgacgctccc ggcgccttcc agaccctgtc
      661 tgatgggttg acccggttcg gcaaccagcc tcagcttctg cggggtcgcg cgatggccta
      721 cctgaaccag aagaattacc cgaaagcact ggctgacttt caagcagtgt acacaattga
      781 accggacccg gttttggctc gtgatatgct cgatctctat cgggcagcaa aattaaccga
      841 ggatggaatt caatttgccc aggttgcatt caagcaatat tcaagcaacg gaccattgcg
      901 ggcggctttt ggggaacttc tgatggaagg caatcggacg accgaagcca tcgaacagta
      961 ttccgaattg accaaacaag aaccgaagaa tgccacgtgg tgggtcaaat tgggagatgc
     1021 ggcacaagcc accgatccag cggtcgcgct gaatgcctac caaaaagccc tctccattaa
     1081 cccccgttcc cctgatgccc aagccggagt cgcatccggt ttgctcaagt cgcaacgctt
     1141 tcaggaagct gcctccagct ttcgcacaat tctcaaccag gacccggaaa gttatgctgc
     1201 ccacgccggg ctggcaacgg cgttgttcaa acttgaacaa ttcccgaccg ccgcccagca
     1261 ctttctgtgg atgatccaga aacgcccgga tgtccccgtg acctactact ttctgggaat
     1321 ctgttttgac cggttgggag attatgccca ggccctgaaa gcctacgagt tgtttgtcga
     1381 acgagccaat ccacaaacca atcagcttga aattgaaaaa gtgaaccttc gtcttcccag
     1441 cctcaaacgc caggttgaga agcaggggcc acgcaaataa gttaaagcga acgagttacg
     1501 agcgaaagac attgaagtga gggtccggcc ctcccttcca aactccttca ctccgtcact
     1561 cctccgctat ttcgttactt cgcttcattc gtcaacttgc tgagcttcca tctcagtcgc
     1621 cttgtcagga tcaaacattc tgggtgaact tttcaggtgc aaacggccag ccataaggcc
     1681 agtcccccaa gatgcaagat cgcggtcaat cccctgacgg aactcggcca cagcagcaag
     1741 gagggacgcc atgcgacacc atcagacaca acatttggtt ctcggaaagg tttggctggt
     1801 ctgggggctg tttttcctgg tgattggaat tcagacaccc ccttgtttgg caagcacgac
     1861 aggcacggtt gaaacgggaa tggaaattcc tgccgtgttg actgaggctg aacggtcagt
     1921 tctggttgag gaaaatgacc cggaagatca cattgaggct ggcctgaaaa tcggattggc
     1981 ccggctcaat caggctgtcc aggcgatgca aaccgaagag tttgacaatg tccatggatc
     2041 gctggcagcc tataaccagc ttttaagtta tctggaatgc tatgcccgcc agtcaccgat
     2101 gaaggcgaaa aaacgcgata agctgttccg gttgatggaa gtcaaactgc ggcaccaatt
     2161 acccttgctc gaagccattg cccgaacctg tccctttgac gaagacaata actcgccgga
     2221 aatgaccctg agccgggttc aaggattgcg caagcaactc ttaaatgtgg tctttggtgg
     2281 cgatttcttc aaaccctgtg aaagtacagc cattaatgcg ttcaaggaat agtcaatcca
     2341 tccagaattg agcaaaaatt tcggatggca tggtggcgag aaaaacctct tgcggatgaa
     2401 tgactttcaa gaggcacgca cgctggtcaa agggatcaac gtgcgtgcct cttttcgttg
     2461 gttcaaccgc tgctcaaggc tgaattggac aggttatggt ctgggcatat tcacctggac
     2521 aacctttcca ttgactgaca gaaaaacatt tcctggttca gccagtaccc cctgccttcc
     2581 ctcccgactt aaggtttcaa gcagtccatt aaattctgga gttccaaatt cgatgatctg
     2641 gtcagctttg agatttttat tctgaaccag tcgcgcatca acccagccat tggaacgtcg
     2701 aaacagggtt tgatcctgaa tctgcctgac gttttgaatt tgggttctct cattttgttg
     2761 tgtcacgtag gcgttggagt agttcaacgt ggtttgcgtc cgctgggcgg cattattgcc
     2821 agtctgggtc atccctccca ccccggttct ggtttgttga gcattgtggg ccagttcgtt
     2881 ttgaacccga ttcaaaactt cgtcgcgccg tgtaagatca gtggtttcgg ttgccaaaaa
     2941 tgccgtgtat tcggtcaaaa tcccaaactc catcgagagc cgaacaattt cgtcggtcag
     3001 ttccttcaag cgcgggtctt tggctgcgct ctgaggatca gcacccgctt tggtgatttc
     3061 ctgaatcaga ctggcaattt ttcgactcgc ccacagccgt gggataaatg aattctgctg
     3121 actggcccgg tcaaatccaa aggtaaaact gaaactttga ggcacaccca gataatttcc
     3181 actcacgtga aacgtcaatg gcgcattggt ctgatatttt ccaagcacca ctaactggtc
     3241 gttttcatag atgtcgttca actcagacgg aaacatctcg cgggcaccac gtgacgtaac
     3301 ctcacctctg gcgctgcgta tttcgagttt cggattgacc atcaatggat tgcccaaccg
     3361 cttgaacaac tctgacacct tcacttcaac atcttcattt ggttggacaa acgtggttac
     3421 ggctcgcgaa tcgggtgcca gtccgtcgag caatggcgaa ttgacgtcat acccgacacc
     3481 gaaagtaaaa attcggcgtt tggactgatt ggcttcgatt gcgttctgac gaatc
//
LOCUS       DAOQDO010000369         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19641_length_3532_cov_2.708944, whole genome shotgun sequence.
ACCESSION   DAOQDO010000369 DAOQDO010000000
VERSION     DAOQDO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19641_length_3532_cov_2.708944"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..702)
                     /gene="ssrA"
                     /locus_tag="PKZ53_07260"
     tmRNA           complement(342..702)
                     /gene="ssrA"
                     /locus_tag="PKZ53_07260"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(930..1277)
                     /locus_tag="PKZ53_07265"
     CDS             complement(930..1277)
                     /locus_tag="PKZ53_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40270.1"
                     /translation="MVEKSVIHECDFKFKCPMYWEELLILDDPDKRYCTECQREVFYI
                     RTREELEEQRQLGHCIAASVNTWGLGLPRIITGGARPLVKKGVEPEKLLILGDERDRN
                     ELRIALRELEKKK"
     gene            complement(1346..1801)
                     /locus_tag="PKZ53_07270"
     CDS             complement(1346..1801)
                     /locus_tag="PKZ53_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40271.1"
                     /translation="MKVQCRLKFQCSQQWEDLSPTSDPNVGFCQLCGQNVYKIDPVNP
                     VIVNEEVKCAAIKTSSPETEGESYHLGDLEWVHDFGYTIMLEPTLSLSDEQMLEIQSI
                     LKTNVDIVALRRVLACGQERTLIYSVPEKEAKQIAARLKKVGVSFKVFR"
     gene            complement(1855..2397)
                     /locus_tag="PKZ53_07275"
     CDS             complement(1855..2397)
                     /locus_tag="PKZ53_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40272.1"
                     /translation="MGKSKKAGVERKPVLLVQEITTRWTKVSRVGPLARVRYTVPEQM
                     PFPVDSVSGSKAQVIFHSVYFDEENSFSAPLEKITASPYVFPGTGCITAGHNGQHAWV
                     TFVQSSGAPIRLHMRKTLWSLPGEWVQMMTNGRYAPGWSGHWWYIKTVVNAGLFFDLD
                     KDVFTRYQPASQFNAMGHLW"
     gene            complement(2602..2949)
                     /locus_tag="PKZ53_07280"
     CDS             complement(2602..2949)
                     /locus_tag="PKZ53_07280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNJ40273.1"
                     /translation="MAIRRGEIYFVNLNPVKGREQSGDRPVLVISTDAINNLPLVVTV
                     VVGTKGANIHQDYLTNVRITPQESGLPLETVFMCFQLRSIDPARFSGPPAGKIGGDTL
                     TRVETAIRYCLGL"
     gene            complement(2949..3182)
                     /locus_tag="PKZ53_07285"
     CDS             complement(2949..3182)
                     /locus_tag="PKZ53_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40274.1"
                     /translation="MPSANLIQLEELIGRLPIIEQLVLIERVSQQIRQRLSSGKNEDA
                     QLAFMAADPEIQTELGKINDEFLVTEADGLEVQ"
BASE COUNT          957 a          884 c          764 g          927 t
ORIGIN      
        1 gtggcagcca gcgcccgacg gccactggtg gcgacccgga cgtgatattt ccgatcaaag
       61 agcatactcg atagcaggtt gaggtttatt ggattgtcat caacgatcaa gatatcgcct
      121 tttgaaccgt tgggtgaact cattcttact ctccattggg ctgaacatga ggaagttgcg
      181 ggcagtggct ggaggagtat agaccactcc ctggaaaaag ccaaaaaatg gcccccatga
      241 actgcagact gattgatttg gatatgggtt caggctcgca cggacgagag tgcaatcaaa
      301 aatatgaata agttgaaagg aaggcaaccg aaaggaggtg aaaatggaga tgaggggagt
      361 cgaacccctg tccgcagacc ttggatggca gagcctacga gcgtagtcgc ttccgtcaga
      421 tttcatcttt gcacagccgg aacagcgacc tgcgcgatgc aaagactaac ccagtgtgtt
      481 ctcgcccgtc acccctgagt acaggtgacg gaccagcctg tgaaagtaac gcccgatcac
      541 gaacgcacag acaacgtgcg tgtcagacgc tagctagctt attaggctgc tagggcgaat
      601 tcctgcgtgt tggcaggttt tgtttccatg caggaacggg ttacatggtt cccggcacgc
      661 ctctggtcac cgcattgccc acgtcgaaac cgtgacatcc ccatattcgg ttgtgaaaga
      721 actgtcttcg atgttacctg cctgtaacac gaggcgtcaa ccgtcaatag caaagcaatg
      781 ccagaatgaa gaattgagaa ttgagaagaa agaatgacct gcttcaggat atcgttttgg
      841 tccttttcgt ccttttggtc cttcatcgcc caaacttgag ccactccaaa gaagccgggt
      901 ggtggtttgg gcgaaattac tgactgaacc tacttctttt tttccagttc cctgagtgca
      961 atccggagtt cattccgatc ccgttcatct cccagaatca ggagtttttc gggttcaact
     1021 ccttttttta caagcggacg cgccccaccc gtgatgattc tcggaagacc cagcccccag
     1081 gtattgacac ttgcggcaat gcaatgccca agttgacgct gttcttcaag ttcttcgcgg
     1141 gttcgaatgt agaagacttc ccgctggcac tcggtgcaat aacgtttgtc aggatcgtca
     1201 aggatcaaga gttcctccca gtacatcggg catttgaact taaagtcaca ctcgtggatg
     1261 acagattttt cgaccataaa aacctccgat ttttcactct cttaattctt aacctttgga
     1321 tcatcagcat ttataaagcc cgtccctatc gaaacacctt aaaactcacc ccaactttct
     1381 ttaatcgggc tgcaatctgt tttgcctctt tttcaggcac actgtaaatc agtgttcgtt
     1441 cctgaccaca ggccagaact cgtctcagag ccacaatatc cacattcgtt ttgagaatgg
     1501 attgaatttc cagcatttgt tcatcactta aactcaatgt cggttcaagc attattgtat
     1561 agccaaagtc atgaacccac tcaagatcac caaggtggta cgattcaccc tctgtttctg
     1621 gagaactggt tttgatggcg gcacatttta cctcttcgtt cacaataacc ggatttaccg
     1681 ggtcaatttt atagacgttt tggccgcaca attgacagaa gccaacattc ggatcagaag
     1741 ttggagacaa atcttcccat tgttgagagc attggaattt taaacggcat tgaactttca
     1801 tcagtatctc cttgcctgaa aacagtcgtc ataaacaact aataacccct ttggttacca
     1861 caaatgaccc attgcgttaa actgactggc gggctggtat ctggtaaaaa catctttgtc
     1921 cagatcaaag aagagcccag cgttgacaac ggttttgata taccaccagt gaccactcca
     1981 gcccggtgca taccgaccat ttgtcatcat ctggacccat tccccaggga gtgaccacaa
     2041 tgtctttcgc atatgtaacc tgatcggagc gccgcttgac tggacaaagg ttacccaggc
     2101 gtgctgccca ttgtgaccag ccgtgatgca cccggttcca gggaaaacat acggactggc
     2161 agtaatcttt tccaaaggag cggagaatga gttttcttca tcaaaatata ctgaatgaaa
     2221 aatcacctgt gcttttgagc cagaaactga atcaactgga aatggcatct gttccggaac
     2281 ggtataccgg actctggcca gtggcccaac acgtgaaacc tttgtccatc tggttgtaat
     2341 ttcctgcacc agcaaaactg gttttctttc aacacctgct ttttttgact ttcccatact
     2401 gttcttttca accgtctttc tttgactctt ctgctttccc agtcaacata ctggtaaagg
     2461 caacgaccag ggttcgttcc caccagataa aatgctgata agtaccttac cgaaaatttc
     2521 cagacatttt gaaccacgaa acacacgaaa aaaccaacta ctcacaaaat tcaacacctc
     2581 aggaaacttt tgacaaagta cttacaaacc aagacagtag cggatagccg tttcaactct
     2641 ggttaatgta tctcctccga ttttacctgc aggtggtcct gaaaatcttg caggatcaat
     2701 tgatcgaagc tgaaagcaca taaataccgt ctcaagagga agtccgcttt cttgtggcgt
     2761 tatccgcaca ttggtcaggt aatcctggtg aatattcgcg ccctttgtgc caacaacaac
     2821 agttaccacc agagggaggt tattgattgc atcggttgaa atcaccagta ctggcctatc
     2881 cccagactgt tctcgacctt tcaccgggtt taaattgacg aagtaaatct ctccacgtcg
     2941 aatagccatc actgtacttc caatccgtca gcttcagtta ccagaaactc atcgttgatt
     3001 ttgccaagct cagtctgaat ttcaggatct gccgccataa aagcgagttg agcatcctca
     3061 tttttaccag agcttaatcg ttgacgaatc tgctgtgaaa cccgctcaat caaaacaagc
     3121 tgttcaatga ttggaagccg gccaatgagt tcttcaagct gaattaaatt tgcagatggc
     3181 ataaccagaa tccctctaac ttttgaaaat acagagcatt gatgattcat tgaagagccg
     3241 aagccataga atactaaaga agtgggtcag ttcaggcaac cagttcaagg ttcaaacagt
     3301 caccaaaggt ggactaacca caaaacagaa ttgagcatca aacacaatat tttctcctca
     3361 gctatgattc aatcagcaca aattctggtg gctcatcatc aacatagagc atcgtgccgc
     3421 actcacattt ccggccacag ggtcgaacta aatttgtctg tgtcgcggcg gacgtttttt
     3481 tgaggcacgt tgggcattga actccaaagt tgattcggcc ccaggatggt gt
//
LOCUS       DAOQDO010000370         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19644_length_3532_cov_2.679896, whole genome shotgun sequence.
ACCESSION   DAOQDO010000370 DAOQDO010000000
VERSION     DAOQDO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19644_length_3532_cov_2.679896"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..3066
                     /locus_tag="PKZ53_07290"
     CDS             259..3066
                     /locus_tag="PKZ53_07290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ40275.1"
                     /translation="MLKSAFNRSDWPVMPALLILGLLLGGSLSLTGCAPARQKPELPD
                     LEELQDQEWEDSAYTSDPELAYEGDEEYEEEIPEGERIPSAMLTVYQSFGGHQTYVSL
                     YVSFPPEKAPSLVEPFQQVFGGKIGKVHERVQKYSIHLSARGDRLLQRSGLVNQFQFD
                     LNPLVTACRTIKLKTLNVYLSHKPINFSEAKGLEVRQVSPYIMAYQKEFNLETPSGLI
                     PPIQLRFGYRVWDVLKVVIPLILLVFLPIGFVLWSLRKAKQVTEHLPRAGAELQRLVE
                     WTMAGYWLIWLSVLDSVIDRNLISFLTGRPRNDTLMVVITLVGPPLLVELCCKLITIP
                     FVSRMRQLQASRREIITLAIVKSIFSFVTWAGLGITLSSLIAQKYLWALMAVSGTLVF
                     WFIGQLVLNSLKRLKQVVLTPGDLRNRVFELATQAGVKVKEIFILSSPKEPLINAFAV
                     AGGNVLLSEDLVSRLNRREVDAIVAHELGHLKHKHPNYLVTGLMLAVVASVYIGSFIK
                     LSGPLAGAQGIVRPVVLLVCLMGYFYLSRRFEFTADAEAVELTKDPQAMITALKEITR
                     HNLMPLEWNQFGEKLLTHPSTRKRAEAIAWKGGIPREHLQALLAESAAEPDNRYELPE
                     VFHAETNEPANLIFSSSLRKQKSRRVLWIQLGIIWGLPMLIGGIVTYRDLAWTSVFPI
                     YLISLVVIPLFFGVALNRLALSRYPHMARLLAQRFNTENKDWHTPRGTFVGLSPSADR
                     QIYDGDYSWDIGFVFLTEERLIYVGERTRFALRRDQIGAIKFHPTQFPWHSSDPVYIT
                     WYDPEQDQGGTFSVTTLNASSYTEVNQKVALFGAQLTQWHQLPASATHSSELFPDLGV
                     PQIAPVVGIQATQIEPRGVIAILALNFLLMLVVCYPTGLDFLGETNAAGWFVALASSL
                     SWLTLFGPVLIANRRKRKSES"
BASE COUNT          855 a          822 c          871 g          984 t
ORIGIN      
        1 gtgaggaatg aagaatgagg aatgaagaat gaagaatgag gaatgaagaa aatctgattt
       61 tctagctaaa aaagtttcca tgacaacatt tttataactt cactctcatc atagaattac
      121 agccaaaagt ttgttttttc cgtacaaaga taattgttgg agtaaaaacg ctctaagtta
      181 attcaataaa gtgatttaag atactttttt attctcaatt cttcattctc aattcttcat
      241 tctcaatggg tattttctat gctgaaatct gcgttcaatc ggtccgactg gccagtgatg
      301 ccagctttgt tgatcctggg attgctcctt ggagggagtt tgagcttgac tgggtgtgct
      361 ccggcacgcc agaaaccgga actccctgat ttagaggaac ttcaggatca ggaatgggaa
      421 gactcagcct ataccagtga tcctgagtta gcctatgaag gcgatgagga atatgaagag
      481 gaaatcccgg aaggcgagcg gattccatcc gcaatgttga ccgtctatca atcctttggt
      541 gggcatcaaa cctacgtttc gctctatgtg agttttcctc cggaaaaagc accgtcgctg
      601 gttgagccgt ttcagcaggt gtttggcggg aaaattggga aggtccacga gcgggttcaa
      661 aaatattcaa ttcacctttc agccagaggt gatcggctcc tgcaacgttc ggggcttgtc
      721 aatcagtttc aatttgacct caacccgctg gtgactgcct gccggacaat caaattaaaa
      781 accctgaatg tctacctgag ccacaaacca atcaattttt ccgaagcgaa gggcctcgaa
      841 gtacggcaag tcagcccata catcatggcg taccagaagg aatttaacct ggaaactcca
      901 tcagggttga ttccaccgat ccaactccgg tttggatacc gggtgtggga tgttctcaaa
      961 gttgtgatac cactgatctt gctggttttc ttgcccattg ggtttgtgct ctggtcgctt
     1021 cggaaggcga aacaggtaac ggaacatctg cctcgcgccg gggctgaact ccagcggctg
     1081 gtggaatgga cgatggccgg atactggttg atctggttgt cggtcctgga ttccgtcatt
     1141 gaccgaaacc tgatttcctt tttgactgga cgcccccgca acgacacgct gatggtcgtc
     1201 atcaccctgg ttggtccacc attgctggtg gaactctgtt gcaaactcat aaccattccc
     1261 tttgtgagcc gaatgcgaca actccaggcg agtcggcgcg aaatcattac tttggccatt
     1321 gtaaaaagca tcttctcgtt tgtaacgtgg gctggactgg gcataaccct gagttcactg
     1381 atagcacaga aatacctctg ggcgttaatg gccgtcagcg gaacactggt gttctggttt
     1441 atcggtcaac tggtgctcaa tagtttgaag cgactcaaac aggtcgttct gactcctggc
     1501 gatttgcgca accgggtttt tgaactcgcc acccaggctg gggtgaaagt aaaagaaatt
     1561 tttattttga gttcgcccaa agaaccgctc atcaatgcgt ttgccgttgc tggcgggaat
     1621 gttttgttga gcgaagacct tgtttcccga ctgaaccgcc gtgaagttga tgccattgtg
     1681 gcccacgaac ttggtcacct gaaacacaaa catccaaact atctggtgac tggcctgatg
     1741 ctggcggtgg tggcttcggt ctacattggg agttttatca aactttcggg gccgctggct
     1801 ggtgcgcaag ggattgtcag accagtggtg ttgcttgtct gtttgatggg gtatttttat
     1861 ctctcgcggc gatttgaatt tacggctgat gctgaggcag tcgaattgac caaagatccc
     1921 caggccatga ttaccgcact caaggaaatc acccgccata acctgatgcc actggagtgg
     1981 aatcaattcg gagaaaagct cctgactcat ccttcgaccc gcaagcgagc cgaagccatt
     2041 gcctggaaag gcgggattcc acgtgaacac ttgcaggcat tgctggctga atccgccgct
     2101 gagccagaca accgctatga actgcccgaa gtcttccacg ccgagaccaa tgaaccagcc
     2161 aacctgattt tttcatcaag tctgcgtaag caaaaatcac gtcgggtttt atggattcaa
     2221 ttggggatta tttggggatt gccaatgttg atagggggca ttgtgaccta tcgggatctg
     2281 gcctggacct cggttttccc aatatatctg attagtttgg tagtgattcc actttttttc
     2341 ggagttgcgc tcaaccgact ggccttgagc aggtatccac acatggctcg gttgctggct
     2401 caacgattca acacagaaaa caaagattgg cacacaccca ggggaacatt tgttggactt
     2461 tctccttcgg cggaccggca gatctatgat ggggactatt cgtgggacat cggctttgtc
     2521 tttttgaccg aagagcggtt gatttatgtt ggagaacgaa cgcggtttgc tctcagacgt
     2581 gatcaaattg gagcgatcaa attccatcca acccaatttc cctggcattc ctccgatccg
     2641 gtttatatca cctggtacga cccggagcag gatcagggag ggacgttttc agtgaccaca
     2701 ctcaatgcca gctcctacac tgaagtaaat caaaaggtag ctcttttcgg cgcccaactc
     2761 acgcaatggc atcagttgcc tgcatctgcc acacacagct cagaattgtt tcctgacctg
     2821 ggagttccac aaatagcacc ggttgtcggc attcaggcca ctcagattga gcctcgcgga
     2881 gtaattgcga ttcttgccct gaattttctc ctgatgctgg tggtgtgtta cccgactggt
     2941 ttggattttt tgggtgaaac caatgcggct ggatggtttg tcgcgctggc ttcaagcctg
     3001 agctggctga cactttttgg gccagtttta attgcaaacc gcaggaagcg aaagagcgag
     3061 tcgtgagtag tcggaagact ggctttattt tctgtgggtt ccgtatcttc cgtggtttct
     3121 tgttctgaaa atatatggag aagcatcaat ccatcaggcc gtgggctcac ttttaaactt
     3181 tgatttatgt ctttgtcacc gtcacaaaac tgcttactgt tttggccagg tacaattttc
     3241 ctgacgactt gctatttgct gttcgctctt gtgcgctact tcgctatttc actcttgatt
     3301 aaattcaatc aattgaaaat ttttacaatt tcctcgattt ttttggagta tgatttccgc
     3361 tcacccacta cctgccatca aaacggtgtg agggaacatc acctctgatt gccttcctcc
     3421 cgccaatcgc aaccaacttt accttgtttg ttgacacaat ttgttttgag ccacctttgg
     3481 aatgaagaat gaagaatgaa gaatgaagaa tgaagaatga agaatgaaga at
//
LOCUS       DAOQDO010000371         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19668_length_3529_cov_2.396085, whole genome shotgun sequence.
ACCESSION   DAOQDO010000371 DAOQDO010000000
VERSION     DAOQDO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19668_length_3529_cov_2.396085"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..3273
                     /locus_tag="PKZ53_07295"
     CDS             493..3273
                     /locus_tag="PKZ53_07295"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNJ40276.1"
                     /translation="MLRKSFLLLFVFFFAYSNCLPIFADEGMWTFDNPPIKQVKDKFG
                     FEMNRAWLDHVRLAACRLNNGGSGSFVSADGLVLTNHHVASSAIAQLSTPQRDLMKNG
                     FYARTRGEELRCPDLEINVLYAYEEVTDRVLRAGRNAGDDRSAEKMRKAEMADIEKES
                     TERTGLKSTVVTMYEGGEYWLYQFKKYTDVRLVFAPEAQAAEFGGDYDNFSFPRFGLD
                     FSFFRVYENDRPARIEHYLKWNSRGAKENELIFVVGNPGKTDRLRTVSQLEYQRDIEN
                     LVMLKGLRRRRKALEEFSEKSAENARRADDALHSINNSLKRLNGQQEGLESDKLMRQK
                     VDEEKELRQKVAASPELQRKFGDAWDQMADAYKKLYPTMGKRVMLSSFSNAPLAAKAL
                     QIVQYVTEVQKPNAERLEAYRESNLESLRFQLLSPAPVYADLEEVLLLTRLQELRDEL
                     GENDPFVQAVLGNKRPREAASDMIQGTKLADVNFRKSILDGGLAALERSNDPLLQAAR
                     RVDPIVRELDQWNKQNIDSVRNAAGEKVARARFAVYGRTLAPDANFNMRLSYGVVKGY
                     EFNTTMLPYKTTFGGLFDREASFDAKPPFNIATRIKASRSRINMDTPVDFVYTADTIG
                     GNSGSPVVNQAGELVGVNFDSNVHRFVSRYIYTEDKGRAMAVHAGGMMETIRNVYDAK
                     GLADELERGSSGSGGGGGSVPAPTTGPGMALSPDQVSVDLMNLANRIRGKVEAAERNN
                     PDEPPFMNGNPEYLRFRFDNDRLESYVTYHERQLVIYPIDAYRAVFKGKERAEFDKQM
                     NALKQLLIRQPVSPIEEITVFPPAEAAQVFHAQVKYLSFDGGKGVRFISRYAQDVTPT
                     TNDSIFYTFQGLTSDGKYYIALFYPIDARGLPETDIVKRTVRFLERLQPTDYDPDLTR
                     LDDMVKSLKIGK"
BASE COUNT          853 a          924 c          941 g          811 t
ORIGIN      
        1 ctggccgccc attattgtct ggtgcacgag cacgaaaccg aaccgatgac gctggtcccg
       61 cattcgctgc tggtggattt tgggaatgtc ctttaaaacc gggctgaaaa aaccagggct
      121 cagggctggg ggctgaagaa ttcgggctga agaactcggg ctggggttgc tttcacgtgc
      181 caggcttttt gattttatcc tggagagatg gagcaagttt agccggttgg ttggccgctt
      241 tgggcctacc accggatcca gaaccaccca ccttgttcct ccccgcacca cccgcgtccc
      301 gttggggcgc gggtggtgcg ggaaaatttg gaaattgacc agatccggtg gtggtgcgtc
      361 caaagcgacg ctgaccactg gctaactttg ctccatccct ccgggatgag aaccaaacac
      421 ataggcgtgc ccgccttgtt tccattactt cactacctca ctacttcact aactcgctaa
      481 ctcgctcatc ctatgcttcg aaaatctttt ctgctgctct tcgttttttt ctttgcttac
      541 tcaaactgcc ttccaatttt tgccgatgaa ggcatgtgga cgttcgacaa tccacccatc
      601 aaacaggtca aagacaaatt tggctttgaa atgaatcggg cgtggcttga tcacgttcga
      661 ctggcggcct gccggctcaa caatggcggt tccggttcat ttgtttcggc ggatggactg
      721 gtgttaacca atcaccacgt tgccagttca gcgattgccc agctttcaac gccgcaacgc
      781 gacctgatga aaaacggttt ttatgcgcgg actcgcggcg aagaactccg ctgcccggac
      841 ctggaaatca acgtcctgta tgcctatgaa gaagtcaccg accgcgtgct gcgcgctggt
      901 cgcaatgccg gtgacgaccg ctcggctgaa aaaatgcgca aggccgaaat ggcggatatt
      961 gaaaaagaat ccaccgaacg caccgggctc aaatcaaccg ttgtgaccat gtatgaaggc
     1021 ggcgaatact ggctctatca gtttaaaaaa tacaccgatg tgcggctggt gtttgctccc
     1081 gaagcgcagg cggcagaatt tggcggcgat tatgacaact tcagttttcc gcgcttcggt
     1141 ctcgattttt cctttttccg ggtctatgaa aatgaccgcc cggcccgaat cgagcattac
     1201 ctgaaatgga acagccgtgg cgccaaagaa aacgaattga tctttgtggt gggcaacccc
     1261 ggcaaaactg accgactgcg caccgtttcc cagcttgaat accagcgtga tattgaaaac
     1321 ctggtcatgc tcaaagggtt gcgacgacga cggaaagccc tggaagaatt ctctgaaaag
     1381 agtgctgaaa atgcacgccg ggccgatgat gcgctgcatt cgatcaataa ctcgctcaaa
     1441 cggctcaatg gtcaacagga agggctcgaa tcagacaagc tgatgcgcca gaaagttgat
     1501 gaggaaaagg aactccggca aaaagtggcc gccagccccg aacttcagcg caaattcggc
     1561 gatgcctggg atcaaatggc cgacgcctac aaaaaactct atcccacgat gggtaaacgg
     1621 gtgatgcttt cgagcttttc gaatgctcca ctggccgcca aagcgctgca aattgtgcag
     1681 tatgtaaccg aagtccaaaa accaaatgcc gaacgactcg aagcctaccg cgaatccaac
     1741 ctggaatcat tgcggtttca attgttgtca ccagccccgg tgtatgccga ccttgaagaa
     1801 gtgttgctcc tgacgcgact ccaggagttg cgtgatgaac tgggtgaaaa tgacccgttt
     1861 gttcaggcag tgttgggcaa taaacggcct cgcgaagcgg cgagtgatat gattcagggc
     1921 acgaaactgg cggatgtcaa tttccgcaag tccattcttg acggaggtct ggcagccctg
     1981 gaacgctcaa acgatccgct cttgcaggct gcccggcgtg ttgatccaat tgtccgcgaa
     2041 ctcgatcagt ggaataaaca gaacattgac agcgttcgca atgcggctgg tgaaaaggtc
     2101 gcccgcgccc ggtttgccgt ctatggccgg acgctcgcgc cggatgccaa tttcaatatg
     2161 cggttgagct atggcgtcgt caaagggtat gaattcaaca cgacgatgtt gccctacaaa
     2221 acaacctttg gcggcctgtt tgatcgggaa gccagttttg acgccaaacc gccgtttaac
     2281 attgcgaccc gcatcaaagc cagccgcagt cgaatcaaca tggacacacc ggttgatttt
     2341 gtctacacgg cagacacgat tggcggcaat tcaggaagtc cagtcgtcaa tcaggcaggc
     2401 gaactcgtcg gcgtcaactt tgacagcaac gtccatcggt ttgtgtcgcg ctatatctac
     2461 actgaagaca aaggccgggc gatggcggtt cacgcgggcg gcatgatgga aactatccgc
     2521 aacgtctatg atgccaaggg attagccgat gaactcgaac gaggttcatc cggcagcggt
     2581 ggcggcggtg ggagcgttcc ggcaccgacc acagggccgg gaatggcgct gtccccagat
     2641 caggtttcgg ttgatttgat gaatctggca aatcgcattc gtggcaaagt cgaggccgcc
     2701 gaacgcaata accccgacga accgccgttt atgaacggca accccgaata cctccggttt
     2761 cggtttgata atgaccggct cgaaagttat gtcacctacc acgaacggca actggtgatt
     2821 tatccgattg acgcctatcg tgcggtgttc aaagggaaag aacgggctga atttgacaaa
     2881 cagatgaatg cgctcaaaca gttgctgatc cggcagccgg tgagcccgat tgaagaaatc
     2941 accgtgtttc ctcctgccga agccgcccag gtgtttcatg cgcaggtgaa atacctgagt
     3001 tttgacggtg gcaaaggcgt gcggtttatt tcacgctatg cccaggacgt tacaccaacc
     3061 acaaatgact cgattttcta cacgttccag ggcctgacca gtgatgggaa atattacatt
     3121 gccctgtttt acccgattga cgcgcgcggt ttgcccgaaa ccgacatcgt caaacgaacg
     3181 gttcgctttt tggaacgcct ccagcccact gactatgacc cggacttgac ccggctggat
     3241 gatatggtga agtcgctgaa gattgggaag tagggttcag ggtttgaaaa gcagggttca
     3301 gggttcaggg ttttcgaatt tatgtccttt atgtcccgtt tgtccttttt gtctttttct
     3361 tgaaaacccg gaacctggga ccgtaaccct aaccaaatga aaggcacggt ggtggacgcc
     3421 gtgcctttta tcatttacag tgaaaaacaa tagcacctgt tttcaagcca gaaattttcc
     3481 ttcatcaatg taaaggtttc aaagtatgtc tgaagtattg agcaccacc
//
LOCUS       DAOQDO010000372         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19838_length_3513_cov_3.188837, whole genome shotgun sequence.
ACCESSION   DAOQDO010000372 DAOQDO010000000
VERSION     DAOQDO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19838_length_3513_cov_3.188837"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKZ53_07300"
     CDS             <1..454
                     /locus_tag="PKZ53_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNJ40277.1"
                     /translation="THLELGWGQSTVIPVEIADLPKLRHLTILNVALNGFPEAVLECR
                     SLRELCIRGADIPRLPEALEQLSNLTTLHIGNCGLVELPSCLSRMHRLKALEFPDNRL
                     TQIPSSLRSLPILRQLFLTGNPLTETTVSDLKDQFPQAIAFSIPGKHK"
     gene            583..1131
                     /locus_tag="PKZ53_07305"
     CDS             583..1131
                     /locus_tag="PKZ53_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40278.1"
                     /translation="MAFSTVAFAVDIEVIRTVYGSNNQTLYWTIEEKFSYELQHNDLW
                     FEEEIQRGAITLRDALMHIIEGRPQDNPVTAFQYGYAVELLCKHFGHFLDGDGLINRI
                     VDLEIQTAILESGVPIPVPPPPDIPIIGHLTSEQVIDEYIHLKEQSLIHPDQYIEDAR
                     QEFRSFLRQAHEKGLGLVSFTY"
     gene            1165..2028
                     /locus_tag="PKZ53_07310"
     CDS             1165..2028
                     /locus_tag="PKZ53_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ40279.1"
                     /translation="MTIHLRPITPADTETCGRIIFEAFKGIADQHNFRPDFPSVEFAT
                     QFAGAFINSPTTFGVAAELDGVFVGSNFLSEDDAIRGVGPITIDPGCQARGIGRKLMQ
                     AVIERGRDAIGIRLVQDAFNTASMSLYTSLGFDIKEPLVMIEGHIQADLPAGIEVRRL
                     AADDFDACGDLCRSVHGIERTNELRHMPPMFPSFAAFRNGRLVAYTSAPHFWPLNHGV
                     AETTEAMQALLIGASQIVPQPLSFLIPIRQSALFRWCLGHGMRVIKPMNLMAMGEYHE
                     PQGCFLPSVGY"
     gene            2468..3061
                     /locus_tag="PKZ53_07315"
     CDS             2468..3061
                     /locus_tag="PKZ53_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40280.1"
                     /translation="MEGIINSVADPIFVKDRTYRFVLLNDAFCEFSGHSREEMLGKTD
                     FDFFPAHQAQIFQEKDEDVFLTGEENINEEELTDRAGNTHTIITKKTLHTTKAGEVLL
                     VGVIRDVTELKRIEREREELIQKLQTALDEVKLLQGFLPICSYCKKIRQDENYWQQIE
                     QYIFDHLGTQFSHSICPDCFTNIAQKELEQLRQRFKR"
     gene            3120..>3513
                     /locus_tag="PKZ53_07320"
     CDS             3120..>3513
                     /locus_tag="PKZ53_07320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ40281.1"
                     /translation="MLVIRDMRIEDVLQVSALVLRSFSAFIAPGLAEEGQAEILNFAR
                     PENLAARHATGHFTLVAEIDEELAGMIQVRCPSHVLMLYVDEKFHRRGIAQYLMQSAV
                     EQIRLEYPEVELITVNSSTYAVTAYRRMG"
BASE COUNT          869 a          903 c          845 g          896 t
ORIGIN      
        1 cacacatctg gaacttggct ggggacaatc caccgtgatt ccagttgaaa ttgccgacct
       61 gccgaaactc cgccacctga caattctgaa cgttgcgctc aacgggtttc ctgaagcagt
      121 gcttgagtgt cgctcacttc gggagctttg tattcgtggc gctgatattc ctcgccttcc
      181 tgaggctctg gaacagcttt ccaatctcac aacgttgcac attgggaact gcggactggt
      241 tgaacttcct tcctgtttga gccgaatgca tcgcctgaaa gcccttgagt ttcctgataa
      301 ccggctcacc cagattccgt cttcgctgag gtcacttcca atcttgcgcc agttgttttt
      361 gacaggcaat ccactgaccg agactacagt ttcagatctc aaggaccagt ttcctcaagc
      421 cattgcattc tcgattcctg gcaagcacaa gtaaattgtt agctgctcac cggataaact
      481 gacttgccaa acaactccgc ttcaagcata aagaatcaaa agtttgcctc gacctcactg
      541 acactgagga gtgtatccaa actcaaggac tccctgaccc atatggcatt tagcacagtt
      601 gcgtttgcgg ttgatatcga agtgattcgc acggtttacg gttcaaacaa tcaaacattg
      661 tattggacga ttgaagaaaa attttcctac gaacttcagc acaatgacct ctggtttgaa
      721 gaggaaattc agcgcggggc catcacgcta cgcgatgcac tgatgcacat tattgaaggc
      781 agaccccagg ataacccggt cacggcattt caatatggat acgctgttga actcctctgc
      841 aaacacttcg gtcatttttt agacggcgat ggcctgatca accggattgt tgaccttgaa
      901 atccaaacgg caatccttga atccggagtc ccgatcccgg ttccgccgcc gccggatatt
      961 ccaattatcg ggcatctgac ttcagaacag gttattgacg agtacatcca tctcaaagaa
     1021 caaagcctga tccatcccga tcaatacatc gaagatgccc gccaggaatt tcgatctttt
     1081 ctgcggcagg ctcacgagaa agggctcggg ctggtgtcct ttacctatta acctctgaca
     1141 tcactcttct tttggaaacg gcgcatgacg attcaccttc gcccaattac cccagcagat
     1201 accgaaacct gtggacgcat cattttcgaa gccttcaaag ggattgccga ccagcataat
     1261 tttcgaccgg atttcccgtc ggttgaattt gccacccagt ttgccggcgc ctttatcaac
     1321 agcccgacga cctttggcgt tgcggcagaa cttgacggag tgtttgtcgg atcaaacttt
     1381 ttatccgaag acgatgccat ccggggggtt gggccgatta cgattgatcc agggtgccag
     1441 gcgcgcggaa ttggccgaaa actgatgcag gccgtgattg agcggggccg cgatgcgatt
     1501 ggaatccggc tcgtccagga tgctttcaac acagcttcga tgtcgctcta taccagcctt
     1561 ggatttgata tcaaagaacc attggtcatg attgaaggcc acatccaggc agaccttccg
     1621 gctggaattg aagtccgacg acttgcggct gacgatttcg atgcctgtgg cgatctgtgc
     1681 cggagcgtcc acggaatcga acgcaccaac gaactccgcc atatgccgcc gatgtttcct
     1741 tcctttgcag cgtttcggaa tggacgattg gtcgcctata cctcagcacc acatttctgg
     1801 ccgctcaatc acggcgtcgc tgaaacaact gaagccatgc aggccctcct gattggcgct
     1861 tcccaaatcg tcccccagcc gttgtccttc ttgattccaa tccgtcaatc agccttgttt
     1921 cgctggtgcc ttggccacgg aatgcgcgtc atcaaaccca tgaatctgat ggccatgggt
     1981 gaatatcacg aaccacaggg gtgttttttg ccgtcagtcg ggtattagga attggggcgg
     2041 aagacatcgg gctgaagatt ctcgggctca gggcggaaga catcgggctg aagattctcg
     2101 ggctcagggc ggaagacatc gggctgaaga ttgttgggtt cagggtattg aactcatttt
     2161 cttcagcccc gagcttgcga gtcattctgc cccaagccct gagcccaatt tctgatgtcc
     2221 tttgtcacac tcctcttttg ggaaatgatc tcagcagtgg atcaattgtg gcagtctcac
     2281 ccgtggatca cgatgagcaa tcttcaaatt acattattat atttccatca gattatacgg
     2341 gtatatttac ttttgattga atgggttgaa atagaatgga ttggcttcta tcacatgcat
     2401 tccagtgcta gtatgtcatc aggtttgagg atttctttaa ccatccccaa ctcaggtcga
     2461 ggtgacaatg gaaggtatca ttaactcagt cgctgatccg atttttgtga aagaccgcac
     2521 ctatcggttt gtgctgctca atgatgcctt ttgtgagttt agtggacatt cgcgcgagga
     2581 aatgctggga aaaacggact tcgacttttt cccggctcac caggctcaaa tatttcagga
     2641 aaaagatgag gatgtcttcc tgactggtga agaaaacatc aatgaagagg aactgactga
     2701 tcgggctggg aatacccata ccatcattac gaagaaaacg ctccacacca ccaaggcagg
     2761 tgaagtcctc ctggtgggtg tgatccgtga cgtaaccgag ctcaaacgca ttgaacgcga
     2821 gcgtgaggaa ctgatccaaa agctccagac agcacttgat gaagtcaaac tgctccaggg
     2881 gtttttgccg atttgctctt actgtaagaa gatccgacag gatgaaaact actggcagca
     2941 aatcgagcaa tatatttttg accacctggg aacacagttt agccatagca tttgcccaga
     3001 ttgctttacc aacattgccc aaaaagaact ggagcaactt cgacagagat tcaaaagatg
     3061 agggttccgg gttttcgata aatctcaaaa aatcacactg aaaagaggtt cgggaacaga
     3121 tgctggtaat tcgcgatatg cgcattgaag acgtgctgca ggtttcagca ctggttctca
     3181 ggtcattctc agcttttatc gcccctgggt tagccgaaga aggtcaggca gaaattttaa
     3241 actttgcccg acctgaaaac ctggctgccc gtcatgctac cgggcatttc acacttgtgg
     3301 ctgaaataga cgaagagttg gccggaatga tccaggttcg ctgtccgagc cacgttctaa
     3361 tgctctacgt tgacgaaaaa ttccaccgtc gcggcattgc ccagtacctg atgcaatcgg
     3421 ctgtcgaaca aattcgcctt gaataccctg aagttgaact catcacggtg aattcttcga
     3481 cctatgccgt caccgcctat cgtcgaatgg ggt
//
LOCUS       DAOQDO010000373         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19863_length_3511_cov_2.992766, whole genome shotgun sequence.
ACCESSION   DAOQDO010000373 DAOQDO010000000
VERSION     DAOQDO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19863_length_3511_cov_2.992766"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKZ53_07325"
     CDS             <1..712
                     /locus_tag="PKZ53_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40282.1"
                     /translation="GTVASGQVKRMELSSFTISSSKERYVLYEPISIKFTIKNDHTQD
                     LNGDFGIAGWRNLEKIGLRYRRENGHEWDYGIEYGEFVVCSGPKIDPLPAHSERSRTM
                     TFLYDRARDQFIFSQPGVYELRARYSYYNFNKKIHSNVLRVVVEPPPPDETEDIIALW
                     KDQYLAQIVQDKEPGAEYQESALGKLKKLLHEYPNSLYTKFAKASILQNTEERKQSLD
                     PIHGYRKTAIELVASFGN"
     gene            912..1805
                     /locus_tag="PKZ53_07330"
     CDS             912..1805
                     /locus_tag="PKZ53_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40283.1"
                     /translation="MSKKFDIPHTAWSLHMVTGSKKTKFSCGLYLFIFFWFSSPIWGQ
                     IPEKGVPTTNSTEVLDAILYPDGAEYRQLTLLKISRPKPSYQEVLSVIQRSQSASRNQ
                     PSTRTSTAVKRKFDPWGFESEFANTMFMVVESPVGNPKEIKQGQVIGLLYGDFDDMTY
                     PLARWSGKLVWDQSHKEGYVVLAKNTAYTTLGVGKFNPKFKISDYPVKSENTEPGKSL
                     LPAGFLSQVTQLTIVVEGGIMGFTRVNALIDQKSIIIQGEDPECTCGHAFLRLNLETK
                     AWTQVFPTEKAVKSEDYINSR"
     gene            complement(1917..>3511)
                     /locus_tag="PKZ53_07335"
     CDS             complement(1917..>3511)
                     /locus_tag="PKZ53_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40284.1"
                     /translation="IATVAGTGYLGYSGDGGLAVNARTTPNSIAFDKKGNLVIGEYSN
                     RAVRHVDLQTGMISTILKPEFLEAGGPAEVIGIQLDGNDNVYIGSKTGIWRLDAQSHL
                     ITLIAGSDRPRENKLEDLLAFTTRIADARALGVDSQGKVYLGVSTGVLQIDPQTSRIA
                     RYAGTGAPGFDGDGGPAEKASLGKVNDLHFLPNDNLLIADGGNVTLQIGPRVRLVDGQ
                     THVITTVAGNGAIVETEENLVALQTPVFPQGITADLSSNPMIGEFYRLRRVDSRTGMI
                     TTLARTHELAGFCAELAVDGGGNIYVVTDLSSTSQVRRIDAETGNLSRVAGNWSPVIS
                     ADGVLATEGGLGWISDLAIDADGNVFLCSSSEGTVRRVDAQTGILTTLPQDPVLKSLK
                     GTLGDLLSITFDTEGNLYQLYLNGFVQVVKGVGRPGKPGIGPVGKDLGQPQPEISSLK
                     IQEKKLILSGKGLDGNSLIVKINGTDMTSRVKLHSGTKLVLKGTPAQLKLYPGTNTIQ
                     LIVNGLPSAIYPFQLVVKQGRP"
BASE COUNT         1002 a          815 c          793 g          901 t
ORIGIN      
        1 ggggacagtg gcttccgggc aagtaaagcg aatggagctt tcaagtttta ctatatcttc
       61 atctaaagaa agatatgtcc tgtatgagcc catttcaatt aagttcacta tcaaaaatga
      121 tcatacccag gatttgaatg gagatttcgg gatagcaggt tggcgaaatc tagagaaaat
      181 tgggttgaga tatcgccggg aaaatggtca cgagtgggat tatggaattg aatatggcga
      241 attcgttgtc tgtagtggtc ccaaaataga tccgttgcca gcacatagtg aacgatcaag
      301 aaccatgact tttttgtatg acagagcacg cgatcaattc atcttttctc aacctggggt
      361 atatgaactc cgagcccgat attcgtatta taacttcaac aaaaaaatac attcaaatgt
      421 tctgagggtg gttgttgaac ctcccccgcc tgatgaaaca gaagatatca ttgccctgtg
      481 gaaagatcag tatcttgcac agattgtgca agataaggaa cctggagcag agtaccagga
      541 aagtgcttta ggtaaattaa agaaattgct tcatgaatac ccaaatagtc tctacactaa
      601 atttgcgaaa gcatctattt tgcaaaatac tgaagaacga aaacagtcac ttgatccaat
      661 acacgggtat agaaaaactg caattgaact ggtggcgagt ttcggaaatt gatctaaacc
      721 tttgttttgt atatgttagc gaatgaatga aagacctaac catacccaaa tataggcatt
      781 gaatgatgga catctcatcg catccaccaa ccaacgccaa tttcagtaca ccttttagaa
      841 acagacttca agaaatctat cttgccatat ccctcacttc ccaccagctc aacaagggca
      901 tccacatatg attgagtaaa aaatttgata ttccccacac ggcctggagt cttcatatgg
      961 taacaggaag taaaaaaact aaattctctt gtggcttata tttgtttata tttttctggt
     1021 tttcctcgcc gatttggggg cagatcccag aaaaaggagt ccccacaaca aattcaaccg
     1081 aggttttgga tgctattttg tatccagatg gggctgaata ccgacaactg acattgctca
     1141 agatcagccg tccgaaaccc tcgtaccagg aagttttatc agtcatacaa cgaagtcagt
     1201 ccgcttctcg aaatcagccc tcaaccagga ccagtactgc cgtaaaacga aagtttgatc
     1261 cctggggatt tgaaagcgaa tttgcgaata ccatgttcat ggtggttgaa tcgccagttg
     1321 gaaatccaaa agaaattaaa caagggcaag taattggact tctttacggt gattttgatg
     1381 atatgaccta tccattggct cggtggtcag ggaaactggt ttgggaccaa tctcacaagg
     1441 aaggatatgt tgtccttgct aaaaatacag cgtacacaac ccttggtgtt ggtaaattta
     1501 atccaaaatt taagatttcg gactatccag tcaagtctga gaacactgag ccaggaaaat
     1561 ctttattacc tgcaggtttt ctgtcacaag tgacccaact aacaatcgtt gttgaaggcg
     1621 gaatcatggg ctttaccagg gtcaatgcgc tgattgatca aaaatcaatc atcatccagg
     1681 gtgaagatcc agaatgtact tgtggtcatg cgttcttacg gctcaacctg gaaaccaaag
     1741 cctggactca ggtttttccg actgaaaagg ctgtcaaatc agaggattac attaactcaa
     1801 ggtaagcctg gttcaataag tttggtttat ttggcgaatc agagatcaaa aattggtgaa
     1861 cagaaataca gaaaggtagt gaatggtcaa tgtctctgat ccgacaaatc cgtagatcaa
     1921 ggtcggcctt gtttcacaac aagctggaat ggataaatcg ccgaaggcag tccattcacg
     1981 atcaattgaa tggtgttggt accgggataa agcttcaact gggcaggtgt gcccttgagg
     2041 acaagtttcg ttccagaatg aagtttcacc cgtgaagtca tatcagttcc atttattttg
     2101 acaatcaacg aatttccatc caatcctttc ccagaaagta tcaatttttt ctcttgaatc
     2161 ttcagcgatg aaatctctgg ctgaggttga cccaggtcct ttccgacggg accgattccg
     2221 ggtttgccgg gtcgcccgac gcctttgaca acctgtacaa atccgttgag gtagagttga
     2281 tacaggtttc cttccgtgtc gaaagtaata ctcagcaggt ctccgagcgt tcctttcagg
     2341 ctctttaaaa ctggatcttg aggcaaggtg gttagaattc cagtctgagc atccacccgg
     2401 cggacagtcc cttcagagga tgaacacaga aagacattgc cgtcagcatc aatcgccagg
     2461 tcggaaatcc accccaatcc gccctcggtt gccagtacac cgtcagccga aataaccggc
     2521 gaccagttgc cggccacacg ggataggttt cccgtttcgg catcaatgcg gcgtacctgg
     2581 ctggttgaag acaaatcggt gaccacataa atatttccac cgccatcaac tgccagttca
     2641 gcacaaaaac ctgccagttc gtgagtgcgc gccagcgtcg tgatcattcc agttcgggaa
     2701 tcaacccggc gaaggcgata aaactcacca atcattgggt ttgaggagag atcagcggta
     2761 atgccctgtg gaaagactgg ggtttgaagg gccaccaaat tttcttcggt ctccacaatg
     2821 gcgccatttc ctgccaccgt ggtaatcaca tgcgtttgac catccaccag cctgacacga
     2881 ggaccaattt gcaatgtgac attcccacca tcagcaatca gtaagttatc atttgggaga
     2941 aaatgcaaat cattcacttt tcccagactc gctttttcag ccggcccgcc atccccatca
     3001 aacccagggg cgccagttcc ggcataccgg gcaatccggc tggtttgggg atcaatctgc
     3061 aagacaccgg ttgaaacccc gagatagact tttccttgag aatccacgcc cagggcccgc
     3121 gcatcggcaa tgcgggttgt gaaggccagc agatcctcca gtttattctc tcggggacga
     3181 tcagaaccgg cgatgagggt aataagatgg gattgggcat caagtcgcca aattccggtt
     3241 ttagatccaa tatacacatt gtcgtttccg tctaattgaa tcccaatcac ctcggcgggg
     3301 ccaccagctt ccagaaattc aggtttcagg atggttgaaa tcataccggt ttggagatca
     3361 acgtgtcgga cggcacgatt ggaatactct ccgatcacca gattcccttt tttatcaaag
     3421 gcgatactgt tgggcgtggt acgggcattg acggccagcc cgccgtcgcc gctatatcct
     3481 aaatacccgg ttccagccac cgtcgcaata a
//
LOCUS       DAOQDO010000374         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19927_length_3505_cov_4.804928, whole genome shotgun sequence.
ACCESSION   DAOQDO010000374 DAOQDO010000000
VERSION     DAOQDO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19927_length_3505_cov_4.804928"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1234)
                     /locus_tag="PKZ53_07340"
     CDS             complement(56..1234)
                     /locus_tag="PKZ53_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNJ40285.1"
                     /translation="MWIDSDRAFTAFCDEAAESPQIGIDLEFQGEGRYVPQLCLVQIA
                     TPHKIVAVDPFRVNIAPLGPILAAPEVQKIFHAGTQDIAILQRETGITPVGVFDTQIA
                     AAFLGFGESVGYVALVSRCLKVFLSKRQQFTDWTRRPLNREQVDYALNDVRYLLALSQ
                     LLAEQLEQRQRWAWVAEACAEMVTQALQTRTPEQAYLRFGKLAGLTRRELGVLRELAA
                     WREYTARAESRPATTILGEDVMRQLCYVLPKTDSELRQIRGFKPNLPPGLREEILLAI
                     RRGLAIPESELPEPFQPRENDADADTMASLLGAVVRAQATSLELAPALITTRDELIRL
                     ANWAAQDVQGVPPDLNLLQGWRASVVGNLLVDILRGKVGIRVDRQSPGSLAMVELPEA
                     "
     gene            1472..1780
                     /locus_tag="PKZ53_07345"
     CDS             1472..1780
                     /locus_tag="PKZ53_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40286.1"
                     /translation="MASVHDLESIPCTCCGKPLMLGSLYYNVSVRVEAAFDGYLNADE
                     LLSEGKSSQSQMNKMLDLIQHTSAETLEGDVSQVVTAQLCVMCKNALLGLVFGYLPSV
                     "
     gene            complement(1976..2767)
                     /locus_tag="PKZ53_07350"
     CDS             complement(1976..2767)
                     /locus_tag="PKZ53_07350"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNJ40287.1"
                     /translation="MSTLLENDKLVIAGREFHSRLLIGTGKYPSFEIMRDAHVRSGAE
                     VVTVAVRRVNLTDRSQESLLDYIDANRFLLLPNTAGCYTAEEAIRTARLAREVLQTPW
                     VKLEVIGDEKTLFPDNEGLVEATRVLAKEGFIVLPYFNDDLITARKLIDAGAVAIMPL
                     AAPIGSGLGIQNLTTMRILRELITTVPIIVDAGVGTASDVALAMELGMDGVLLNTAVA
                     TAQDPVKMAEAMKLGIEAGRLAYLAGRMPRRLYASASSPLDGLVR"
     gene            complement(2838..3044)
                     /gene="thiS"
                     /locus_tag="PKZ53_07355"
     CDS             complement(2838..3044)
                     /gene="thiS"
                     /locus_tag="PKZ53_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNJ40288.1"
                     /translation="MHVTLNGESHTFDNPISLSELVVYLGLRPERLAIEVNGSICPRA
                     LWATTCLQDADRVEIVHFVGGGEF"
     gene            complement(3067..>3505)
                     /locus_tag="PKZ53_07360"
     CDS             complement(3067..>3505)
                     /locus_tag="PKZ53_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40289.1"
                     /translation="QPQPIRSGEPAGRPETGSLSFGKTQAFREFDLDRIISDYLQTES
                     KEEKVEVRLLGNTAFYAGETVVLQVEVTRGQNRAPLGGAPVIVKVLGTSFKPQTHSMM
                     TGPDGMAMTTITLPNFTAGSAAIVVQTSSDAGEAEIKQLIRRR"
BASE COUNT          880 a          966 c          865 g          794 t
ORIGIN      
        1 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc cgatgctatg
       61 cctcaggaag ctcaaccatc gccagactgc ctgggctctg gcgatccacg cggatgccca
      121 ctttgcctcg caaaatatca accagcaggt tccccaccac tgaggcacgc catccttgca
      181 acaaattcag atcaggtgga actccctgca catcctgggc agcccaattg gccagccgga
      241 ttaattcatc acgggtggta atcaatgcgg gagccagttc cagggaggtg gcctgtgccc
      301 gaaccacggc gccaagcaac gacgccatgg tgtcagcatc ggcatcattc tcacgcggtt
      361 gaaacggctc ggggagttct gattctggga tggctaatcc acgccgaatg gccagcagga
      421 tttcttcacg taaaccaggc ggaagattgg gtttaaaccc acgaatctgg cgaagttcag
      481 aatctgtttt cggcaggacg taacacagtt gccgcataac atcctcaccc aaaatcgtgg
      541 ttgccggacg tgattcggcc cgggcggtgt attcgcgcca ggcagccagt tcacgcaaca
      601 cacccaactc acgacgtgtc aatccggcca gttttccaaa gcgcagatag gcctgttccg
      661 gagttcgagt ttggagtgcc tgggtcacca tttcagcaca ggcttcggcc acccaggccc
      721 accgctgtcg ttgctccaat tgttcagcca gtaattgtga cagagctaac aagtagcgca
      781 cgtcattgag cgcatagtca acctgctcgc ggtttaaggg tcggcgtgtc caatcagtaa
      841 attgttgccg cttgctcaaa aagactttca aacatcgcga gaccagtgcg acatatccga
      901 ccgattcccc aaacccaaga aacgccgcag caatctgggt atcaaacaca cccaccggtg
      961 tgataccagt ttcccgttgt aaaatggcaa tatcctgagt tccagcatga aatatttttt
     1021 gaacctcagg tgcggccaga attggtccca gcggggcaat attgactcga aacgggtcaa
     1081 ccgccacgat tttgtgtggc gtagcaattt gcaccagaca aagctggggc acatatcgcc
     1141 cctcgccctg gaattccaga tcaattccaa tctggggcga ttcggcagcc tcatcacaaa
     1201 aggcagtaaa ggcacgatct gaatctatcc acattcttca atcacacttt ctgttgtgga
     1261 aattgggatc caaaaatccc tggttctcat gtgtattcgc ggtgtgatgt ctattcaaga
     1321 ccagacatct tgagtttttg agaagaacag atgcctcaaa gtttctgaat atgtgtcatt
     1381 ggggactgag attccactcg tcatcactga tcttcacagg tacaattcga accgacccca
     1441 gcctgtcatt cctcttggcg agaagcttcc catggccagt gtgcacgacc ttgaatcaat
     1501 cccctgtacc tgttgcggca aaccactgat gctcggttca ctttactaca atgtcagcgt
     1561 ccgcgtcgaa gccgcctttg atggatactt aaatgcggat gaattgttga gcgaaggtaa
     1621 atcaagccag tcgcaaatga acaagatgct tgatctgatc cagcatacct cagccgaaac
     1681 ccttgaaggt gatgtcagcc aggttgtgac cgcccaactc tgtgtcatgt gcaaaaatgc
     1741 actcctgggg ctggttttcg gctatcttcc ctcggtgtag tgtcatccag ttctgagcga
     1801 gtagcaagtg gtggggtgtc aaaccacaaa gagtaaatga ttgaaatagt tagttttcct
     1861 tgaactctaa acctgacatc ttcaactcta agtggtatta catacaaaag cctgccgcca
     1921 atggtgttca agaccagaag gggcaggctt ttgggtgatt ttgatgagtc ccgcgttagc
     1981 ggaccagccc gtcaagtgga ctgctggcac tggcatagag ccgtcgcggc atacgtcctg
     2041 ccagataggc aagccgtcca gcttcaatgc ccagcttcat cgcttcagcc attttgactg
     2101 gatcctgggc tgtggcaacc gccgtgttta ataaaacccc atccattccc agctccattg
     2161 ccagcgccac atccgaagcc gtcccgacac cggcatccac aataattggc acggtcgtga
     2221 ttaattcacg caagatgcgc atcgtggtca ggttctgaat tcccaatcca gagccgattg
     2281 gagccgccag tggcataatc gccacggcac cagcatcaat gagcttgcgg gccgtgatca
     2341 gatcatcatt aaagtacggc aatacaataa agccttcttt ggccagcaca cgtgtcgctt
     2401 caaccagccc ctcattatca gggaacaatg tcttttcatc gccaatcact tcaagtttga
     2461 cccagggcgt ttgcaaaact tcacgcgcca gccgggcagt tcgaattgcc tcttcagccg
     2521 tgtaacaccc ggctgtattt ggaagcagta aaaaccgatt ggcatcaatg taatcaagca
     2581 gtgattcctg tgagcgatca gttaaattga cccgtcgcac agcaaccgtg acgacttcag
     2641 cgccggatcg cacatgggcg tcacgcataa tttcaaaact gggatatttt ccagtcccaa
     2701 tcaacagacg ggagtgaaat tcacgtcccg caattaccag cttgtcattt tccagaagtg
     2761 tactcataag aactcgcgag aaaggaaaaa cggaaaagaa tcaggcggtg ggccgcccgc
     2821 accttctgtg gtcatcatca aaactcgccg ccaccgacaa aatggacgat ttcaacccgg
     2881 tcagcgtctt gcaggcaggt tgtggcccac agcgcgcggg gacagatcga gccatttacc
     2941 tcaatggcga gccgctcagg tcttaaaccg agatagacca ctaactcact gagggaaatg
     3001 gggttgtcga aggtatgaga ttctccgttg agtgtaacgt gcatggggag gaaggaggtt
     3061 gaaaaattag cgacggcgga tcaactgttt aatttcagct tcgccggcat ccgaagaagt
     3121 ctgaaccaca atggcggcgc tcccagccgt gaaattcgga agggtaatcg tggtcatcgc
     3181 catgccatcc gggccagtca tcatcgagtg ggtttggggt ttgaatgaag taccgagcac
     3241 tttcacaatc accggggcac cgcctaaagg agcccggttt tgaccacggg taacttcaac
     3301 ttgaagaacc accgtttcgc ccgcataaaa cgccgtgttt ccaagcaggc gaacttccac
     3361 tttttcttct tttgattcgg tttgcaggta atcagaaata attcgatcca ggtcaaactc
     3421 acgaaacgcc tgtgtctttc caaaactcag gcttccggtt tcaggccgtc cagccggttc
     3481 acctgagcga atcggctgag gctgc
//
LOCUS       DAOQDO010000375         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_19933_length_3505_cov_3.232754, whole genome shotgun sequence.
ACCESSION   DAOQDO010000375 DAOQDO010000000
VERSION     DAOQDO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19933_length_3505_cov_3.232754"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKZ53_07365"
     CDS             <1..443
                     /locus_tag="PKZ53_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ40290.1"
                     /translation="RALAGGCGLASACDVILAAESAQFGYPEVKIGFIPAMVMAILRR
                     NISEKRAFDWMAIGDPLSAQEAANYGLVTHVYPDAEFETNALAYVTTLASRSASALYL
                     MKRLLYHMDGMTFESAIRSGVDGNVLSRSTEDCQAGVAKFLKKN"
     gene            complement(544..>3505)
                     /locus_tag="PKZ53_07370"
     CDS             complement(544..>3505)
                     /locus_tag="PKZ53_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ40291.1"
                     /translation="LPTRNFQQLLTLSAGTSTNITNTSEVGRGDTAINVNGQRTTSNS
                     VLINGIDANSIGTGSTPNLAVPATDALQEFIVQTSQFDASQGRNAGGIVTAVTKSGTN
                     EFHGNAYYFLRNEKLNANNFFLNGQGVDRPKNNRHQFGGTFGGPIVKDKVWFFGSYQG
                     TRETNGTSLTNSLATAFTSPDLTDDRSLTALAALSRTRAPLFGGYVNPVSIGLLQARY
                     PNGEFLIPSGNGRSFRTLTSESTFDEDQFNANVDAQVTSNNRLGVKFFFANNSLDQGL
                     FSQFGLANALQTPGYPVATTADNRFLSVTDTHVFGSGIVNEFRFGYNAIPTTAIPEEP
                     FTAAQFGISTPNQGNFSGLPAIALLNQFTVGPSGFSQSDNQADTLSFGDTVTWTKGRH
                     SMKFGGEYRYNRVRLDFDIFARGSITFTGGVAAALAASRPGSITRPDAFTELLVAPTL
                     ASNGQLFGLPSIFSIIGPGSAQRAVRSHDHAFFFQDDYRVNNKLTLNLGVRYDYYGAF
                     YDTEGRFVTFDDTVPGAGRVGTFNGFFQADNAETPLAGIPTLRKSLVDPDRNNFAPRF
                     GFAYNPFDSGKFVIRGGYGVYYDRPNARAANNQGLSFPYYTIASILPGTPFSRPFSSP
                     FAPIPQQYPLNLQNRANFPTGGLPFFAGNVPVPVQGIYPGLHAARTPYIQQYTLGFQW
                     EFMPNTQLEVTFVGSQGRKLSRLRNVNTLRGPNQVQTGPFSPSLSEVVVQGFGVHLTE
                     NTAKSNYNSLQVSLTRRYSNGLQGLVAYTYSHAIDEYSGQGSSNGTSDVSADFGNQGN
                     FAGNRATADFDRRHRLVVSMVYDLPAIYKGDNVIGKSLVNNWQLSGVFTGQTGSPFSV
                     INTAGLFDSSRASYNPLFGGDPNGSGPDKERLNRYFNTSAFILSSGNGNFGTTGRNIL
                     TGPGQTNTDISVVKFIPIKERHKVEFRAEFYNIFNQTNFANPVNVRANPNFGRIVSTS
                     TGPRLIQFAFKYSF"
BASE COUNT          864 a          874 c          965 g          802 t
ORIGIN      
        1 gccgtgccct ggccggaggg tgcgggctgg catcagcctg tgatgtcatt ttggcggcgg
       61 aaagcgctca gtttggctat ccagaagtca aaattgggtt tattccggcc atggtcatgg
      121 cgattttgcg ccgtaatatc agcgaaaaac gagcttttga ctggatggca attggcgacc
      181 ccctttcagc ccaggaagca gccaattatg gactggtcac ccacgtctat ccagatgctg
      241 aatttgaaac caacgccctg gcatacgtca caacactggc ctcccggagc gcgtcagccc
      301 tctatttaat gaaacgcctg ctctatcata tggatggaat gacgtttgaa tcggcaatcc
      361 gaagcggggt tgacggaaac gtgctcagcc gcagcactga agattgtcag gcgggagttg
      421 ccaaatttct caagaaaaac taacgacacg gcctgtcaga cacaacactc cacagagaaa
      481 aagggagaca gttaaacact gtctcccttt tcacattttc caatcacaaa cgaagtcgtt
      541 tgattagaag ctgtacttaa acgcaaactg gatcaggcgt gggccagttg aggtgctcac
      601 gatccgtcca aagttggggt tcgcacgaac gttcaccggg ttggcgaaat tcgtctggtt
      661 gaagatgtta tagaattcag cccggaactc aactttgtga cgttctttga tcgggatgaa
      721 tttcacaact gaaatgtcgg tgttggtttg gccagggcca gtcaaaatgt tacgaccagt
      781 ggtaccaaag tttccattcc cgctcgacag gataaaggca ctggtattga agtaccggtt
      841 gagccgttct ttatcgggtc cgctgccatt tggatcgccg ccaaacaacg gattatagct
      901 ggcacgtgat gagtcaaaga ggccagccgt gttgatcacg ctaaagggtg atccggtttg
      961 accagtaaag acccctgaaa gctgccagtt gttgaccagc gacttcccga tcacgttatc
     1021 gcctttgtag attgccggca ggtcatagac catgctgacg accaggcggt ggcgacggtc
     1081 aaaatcagcc gtggcccggt ttccagcaaa attcccctgg tttccaaagt cagccgatac
     1141 gtcgcttgtt ccgtttgatg atccctgacc ggaatattcg tcaatggcgt gtgaataggt
     1201 gtaggccacc aatccctgca gtccgttgga ataccgtcgg gtcaggctca cttgcagtga
     1261 gttgtaattg gacttggctg tattttcggt caaatgcaca ccaaagcctt gaaccacaac
     1321 ttcagacagc gatggagaga aaggtccagt ctgaacctgg tttggtccac gcaaggtgtt
     1381 gacattgcgc aaccgtgaca gtttgcggcc ttgtgagccg acaaaggtca cttccaactg
     1441 ggtgtttggc ataaattccc actggaagcc cagggtgtac tgttgaatgt atggtgtccg
     1501 ggcggcatgg aggccagggt aaatcccctg caccggaacc ggaacattac cggcgaagaa
     1561 cggcaaacca cccgtcggga agttcgcgcg attttggagg ttcaatggat actgttgcgg
     1621 aatcggagca aacgggctgc taaatggccg tgagaatggc gtgccaggca gaattgaagc
     1681 aatcgtgtaa tacgggaagc tcaagccctg gttgttggcc gcacgggcat ttggacggtc
     1741 atagtacaca ccatatccgc cacgaatcac aaacttgcct gagtcaaatg ggttgtaggc
     1801 aaacccaaac cgtggtgcaa aattgttccg gtcaggatca accagcgatt tacgcagggt
     1861 tggaatcccg gccagtggtg tttcagcatt gtcagcctgg aagaaaccat tgaaggttcc
     1921 gacccggcca gcacctggca ccgtgtcgtc aaacgtcaca aaccgacctt cggtgtcata
     1981 gaaggcaccg tagtagtcat accgaacacc cagattcagg gtcagcttgt tgttgacccg
     2041 atagtcatcc tggaagaaaa acgcatggtc gtgtgaccga accgcacgct gggctgaccc
     2101 aggaccaatg atcgagaaga tgctgggcag accaaacaat tgaccatttg aagccagcgt
     2161 tggggccacc agcaattcgg tgaacgcatc tggacgggta attgagcctg gacggctggc
     2221 ggccagcgcg gcggccacac caccagtgaa ggtgattgaa ccgcgagcaa aaatgtcaaa
     2281 atccaaccgc acccggttat agcggtattc accgccaaac ttcattgagt gtcggccttt
     2341 ggtccaggtc acggtgtcac caaatgacag cgtatcggcc tggttgtcgc tctgtgaaaa
     2401 tcctgatgga ccaaccgtaa actggtttaa cagagcaatg gctggcagtc cgctgaaatt
     2461 cccctggttg ggggtgctga ttccaaactg agccgcggta aacggttctt ctggaatggc
     2521 ggtggttgga atggcgttat agccaaaccg gaattcattg acgatgccgc ttccaaacac
     2581 atgcgtgtca gtcactgaca aaaagcggtt atcggcagtg gtcgccaccg gatagcccgg
     2641 cgtttgtaag gcatttgcca atccaaactg gctaaacaat ccctgatcca acgagttgtt
     2701 ggcaaagaag aatttgacac ccaaccggtt gttgctggtg acctgggcat caacgttggc
     2761 attgaactga tcttcgtcaa aagttgattc agaggttaag gttcggaatg aacggccatt
     2821 tccagaagga atcagaaatt ctccgttcgg gtatcgagct tgcagcagtc caatcgaaac
     2881 tggattgaca tatccaccaa acagtggagc ccgggtccgg gacagtgccg ccagggcagt
     2941 caatgaccga tcatcggtta aatctggtga cgtaaaggcg gtggccagac tgttggtcaa
     3001 tgacgtgcca ttggtttcac gcgttccctg gtatgaacca aagaaccaca ctttatcttt
     3061 gacaatcggg ccaccaaagg tgccgccaaa ctgatggcga ttgttcttgg gccgatcaac
     3121 gccttgcccg ttgaggaaga agttgttggc gttcagcttt tcattgcgaa gaaagtagta
     3181 cgcgttgcca tggaattcgt tggtgcccga cttggtcacc gccgtcacaa tcccgccggc
     3241 attacgtccc tgagacgcat caaactgact ggtttggacg ataaattcct gaagcgcatc
     3301 ggtggctggc accgccagat tgggggttga accagttccg attgagttgg catcaattcc
     3361 gttgatcagc actgaattgc tggtcgtacg ctgaccgttc acattgatgg cggtatcgcc
     3421 acggccaact tctgaagtgt tggtaatgtt ggtggatgtc ccagccgaca aagtcaacaa
     3481 ctgctggaag ttgcgggttg gcagc
//
LOCUS       DAOQDO010000376         3496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20040_length_3496_cov_2.484743, whole genome shotgun sequence.
ACCESSION   DAOQDO010000376 DAOQDO010000000
VERSION     DAOQDO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20040_length_3496_cov_2.484743"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..>3496
                     /locus_tag="PKZ53_07375"
     CDS             314..>3496
                     /locus_tag="PKZ53_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ40292.1"
                     /translation="MRRYVQTWVLCLVLGCLVSPVCNTPLYAQSQVNAADLLGKVADP
                     NGATLAGVKVTLRNPQTGYTRETTSKEDGTYQFLAVPSGKYEIAAEAEGFSKYLSQDI
                     SLTIGQVAKFEVVMQVGSVTDEVITVTATQLIETGRTSVAETINQTAINNLPSNGRSY
                     VNFTLLTSTTTRGNQPSLGPAPTSGLNFGGQRARANNVSIDGADATDNATNGVRATVS
                     QEAVQEFQIQTNSYAAEFGRASSAVINIVSKTGSNDIHGNVFAFLRDQAVSARNPFSL
                     DPDYPATRFQGGFTLGGPIKRDRTFFFLAFEATRRQETGFNQIGNDNFGLSAVSPGDL
                     AAAVGPFPALLTPAQQAFVQNSAVPFATRATYFGLAREASNVALTGRTLSGLPIFPAT
                     RVPLPGSYVALSSLLGNYPQSEKTYFPSLRIDHRFNNNNNFFVRGSFTSTTVTGTPSN
                     GQNQPTGINALSRTGFTGSRDFSLVAQNVTTGTSYVNEARFQYARRGLNYGPQGSTVA
                     VEVSGFASFGREPFSPVYRTEQRFQATDNMTLIRGNHTIKFGVDYNLVKTNALFEVNF
                     SGLYSFNNLLVTDPLLASLQLPANAPPFSPVQSYGLGFPESFVQSFGDPTSVFNNPEI
                     GAFVQDSWKITPHLTLNYGVRYDVELTETFPAVTELNRMAEDYLNIQQGLPRDYNNVA
                     PRIAISWDPFKDGKTAIKAAYGLFYGRPLLGVSFLSDVNDGAQSPFLAVPGLVGGVNL
                     FQGAQITPGPGPSVPGYLQGQQKFDPFNPALGNQATLLQISPILPQTLHLSRNFKYDY
                     TGQANLSIERQITSDIGMGVTYSFIKGSQLLRPRNINQANQDLLFQFRRALAGDRSSP
                     LFALAQIVGPGGELIFNQFRATGPNFAFAQAQGLPEPTVRQLAAAFGLPQVPGMFIPF
                     FNVKNYESSGSSVYHALTVNVNKRFSNNFQLLGSYTWSHAIDDSTDIQTQQEPQDNSN
                     PGQDRSNSNFDQRHRFVFNSVFQSPFKRKDGGALNYFLADWTFSPIIELSSGRPFNIF
                     TNDDRTAVNSSSTARPNVVPLGTPGSFPSPD"
BASE COUNT          786 a          985 c          860 g          865 t
ORIGIN      
        1 tcgaactttt ggcctggttc atctcttttg ttttcctctg aaccttgaac cctaagaatt
       61 tgtcctaaga aacaaaacat cttcttgcgc ggggttttgc cggcgtgcta gaaaagatcg
      121 ttccaatttg ttcattttcc cgcatttgcg accttttcgg tcatttgtca tcagttcaac
      181 tccaattcgt ttcgtggcac agccaatcaa gtctgtttca cccgctcttc tgtctgattt
      241 cgccgcattc agaggtcatt ttttcaaggg attttccaat ctcactaaga atttgaaggg
      301 agacgaagct tgtatgcgca ggtatgtcca aacgtgggta ctgtgtctgg tgctcgggtg
      361 tttggtaagc ccggtctgca ataccccact ttacgcccaa tctcaggtta acgccgcgga
      421 cttgctcgga aaggtggctg acccaaacgg ggccaccctg gccggtgtca aagtcacgct
      481 ccgcaatcct caaaccgggt acacccgtga aaccacgtcg aaagaagacg gcacctatca
      541 atttttggcc gtaccttccg gcaaatatga aattgccgcc gaagccgaag ggttttcaaa
      601 gtatctcagc caggatattt ccctcaccat cggtcaggtc gcaaaatttg aagtggtaat
      661 gcaggtcggg agtgtcaccg acgaagtgat tacggtgacc gcaacccaac tgatcgaaac
      721 cggtcgaacg tcggttgccg aaaccatcaa ccagactgcg atcaataatt tgccgtcaaa
      781 tggccgcagt tatgtcaatt tcacgttgct gacttcgacg actacgcgtg gcaaccagcc
      841 gtcactgggt ccagcaccga cttcggggtt gaactttggt ggtcagcgcg cccgggccaa
      901 caacgtttca attgacgggg ctgatgccac cgataacgcc accaatggcg tgcgggccac
      961 ggtttcacaa gaagccgttc aggaatttca gattcagacc aacagttatg ccgctgaatt
     1021 tggccgggcg tcatctgccg tgatcaatat cgtgagcaaa accggcagta atgacatcca
     1081 tggcaatgtc tttgcctttt tgcgcgatca ggctgtttca gccaggaatc cattttcgct
     1141 tgatccagat tatccggcca cccggttcca gggcggattt accctgggcg gccccatcaa
     1201 acgggaccgt acctttttct tcctcgcctt tgaagccacc cggcgccagg aaaccgggtt
     1261 taaccagatt ggcaatgaca actttgggtt gtcagccgta tcacctggcg atctggctgc
     1321 cgcggtcggg ccattcccgg ccctcctgac acctgctcag caggcatttg ttcagaactc
     1381 cgccgtgccg tttgccaccc gtgcgaccta tttcggactg gctcgcgaag cttcaaatgt
     1441 ggcattgact ggccgcacgc tcagcggatt gccgattttc ccggccacgc gggtgccatt
     1501 accaggcagt tatgtcgcac tcagttcact gctcgggaac tatccacagt cggaaaaaac
     1561 ctatttcccg tcactgcgca ttgatcaccg gtttaacaac aacaacaatt tttttgtccg
     1621 gggaagcttt acttcgacca cggtgaccgg aacaccgtca aatggtcaaa accagccgac
     1681 ggggatcaac gccctgtcgc gcaccgggtt taccgggtcg cgtgacttct cgttggtggc
     1741 tcaaaacgtg accaccggga cctcctatgt caacgaagcc cgcttccagt atgcccgccg
     1801 cggattgaac tatggtccgc aaggatcaac cgtggcggtt gaagtctcag ggtttgcgtc
     1861 atttggccgc gagccgtttt caccggtgta ccgaaccgaa caacgcttcc aggccaccga
     1921 caatatgacc ttgattcggg gcaaccacac catcaagttt ggtgtggact ataacctcgt
     1981 caaaacaaac gctttgtttg aagtaaattt cagcgggttg tactcgttta acaacctgct
     2041 ggtaacggat ccattgctgg cctcgctcca actccccgcc aatgccccac cgttttcgcc
     2101 agttcaatcc tatggattag ggtttccaga gtcgtttgtt caatcgtttg gcgacccaac
     2161 cagcgtgttt aacaacccgg aaatcggcgc gtttgtccag gatagctgga aaattacgcc
     2221 acatctgacg ttgaactatg gggttcgcta tgacgtggaa ttgactgaaa ccttcccggc
     2281 agtaaccgaa ctcaatcgga tggcggaaga ttatctcaac atccagcagg ggttgccacg
     2341 cgattacaac aacgtggccc cgcgcatcgc catttcgtgg gatccgttta aggatggaaa
     2401 aaccgccatt aaagccgcct atggtttgtt ttatggccga ccactcctgg gcgtatcgtt
     2461 tctgtctgac gtcaatgacg gtgctcagtc accatttctg gctgtgccgg gactggttgg
     2521 cggcgtgaat ttgttccagg gcgctcaaat tacccccggt cctggcccat cggttcctgg
     2581 atatttgcaa gggcagcaaa agtttgaccc attcaatcca gccttgggaa atcaagccac
     2641 cttgctgcag atttcgccaa ttttgccgca aaccctgcac ctgtcgcgca atttcaaata
     2701 tgactacacc ggacaggcca atctttcgat tgaacgtcaa atcacatctg acattggtat
     2761 gggtgtgacc tatagcttta tcaaagggtc acagttgctg cgtccacgaa acatcaatca
     2821 ggccaaccag gacttgttgt tccagttccg gcgggcgctg gccggtgatc gttccagccc
     2881 actgtttgcc ctggcccaga ttgtcggtcc gggcggggaa ttgattttca atcagttccg
     2941 ggccacgggg ccaaactttg cttttgccca ggctcaagga ttgccggaac caaccgttcg
     3001 ccaactggcg gcggcgtttg ggttgccgca ggtgccaggg atgttcattc cgtttttcaa
     3061 tgtgaagaac tatgaatcaa gcggaagttc ggtctatcac gcgctgacag tgaacgtcaa
     3121 caaacgcttt tccaacaact tccagttgtt gggttcctac acctggtcac acgcgattga
     3181 cgattcaacc gacattcaaa cccagcagga accacaggat aacagcaatc cgggtcagga
     3241 tcggtcaaat tcaaactttg accagcgcca ccggtttgta tttaacagtg tgttccaatc
     3301 gccattcaaa cgcaaagatg gtggagcgtt aaactacttc ctggctgact ggacgttctc
     3361 gccaattatc gaactgtctt caggtcgtcc attcaatatt ttcaccaatg atgatcggac
     3421 tgcggtcaac agctccagca ccgctcgacc aaatgtggtg ccactcggca caccgggttc
     3481 gttcccctca ccggac
//
LOCUS       DAOQDO010000377         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20066_length_3493_cov_3.671611, whole genome shotgun sequence.
ACCESSION   DAOQDO010000377 DAOQDO010000000
VERSION     DAOQDO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20066_length_3493_cov_3.671611"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3312
                     /locus_tag="PKZ53_07380"
     CDS             <1..3312
                     /locus_tag="PKZ53_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40293.1"
                     /translation="GLTSEIISALFEDRQKRLWIGTRKGLNYLSLESPNQGFKRVDTL
                     DGKAVTDIRAIGQSADGSILLGSQNMLFQVINRTDALALTSYKTLEGQIVSLFTDQKQ
                     NLWIGTIGAGLYQERPDHTLTHFTNATGFSGTVASVFCEDRDGNLWIGSAKIGLVRFA
                     DNKFTAFTQSDGLSGEDVSSLLEDREGNLWVGTYRDGLNQLQNGKFLTYTTKEGLSHN
                     IVWQIQEAQDHSLFICTNQGLTHLTNGKLTRFVTGYFNSTVYCAVQDQSGNWWAGTQS
                     NGLVQFHPDLPNRPPLATFTTQTGLPSNQVRALSEDTQGNLWLGTPAGLCRKSGATIT
                     TFTTENGLPLNVILAITCAKDGSVWVGTDGGGLTRFQNGQFTTFTRTEGLPSNVILSL
                     YEDVDGILWVITNAGLCYVQEGKLRTLSQLPGNQPETLHQIIEDTQGNFWIGSGDGIL
                     RVNRKALLQFAQGQMPAGEAALFTQFDGMKTDETNVPAPALRASDGTLWFPTLCGLAI
                     IDPVSIPINPVPPTVVMESVTANGEALRPGPDKLALASFQNTFEVTCSVLSFSAPQRN
                     RIKYRLAEFQSQWSEVQGKRTLSFFNIPSGKYTLEILACNNDGVWSMLPNRLAVHIQT
                     SPWRSPWAILVYSGVVIGLIFSSARLRTGYLVRQNQRLQVQVGERTRKLAETAFHLWQ
                     QQQELEHQKNVLAEANLKLQEIDRLKASFTAMLAHDLKSPLSVVKTAIELLQMESSIA
                     SGPLASLLQASDRSLDKVLTLISEMLEVSRAEAQEMKLSCAPLETESFLRQCAEDVRV
                     SARARRITLEIKLEPGLPAISADRGKLERVFANLLSNALKFTPDGGTISIEGWVETGT
                     GVETGQQLLKVSITDTGEGIPAEDIPYLFEPYRQSKSTKKQLGVGLGLTIVRRIVAAH
                     GGNISVHSQLGVGTCFTVLLPTLPPEASSLAEVSPLSVATEDKSFVSAVPNSPDANSI
                     PPISPRVLIAEDNVISQRILAAQVKKLGYQVEVVNNGLEAVNAVETSRFDAILMDCYM
                     PEMDGFTATQAIRRKKTHRSKIRIIALTAETTVETVEKCLQIGMDDFLGKPFTFEDLK
                     ALLQRWVPIIATEEG"
     gene            complement(3309..>3493)
                     /locus_tag="PKZ53_07385"
     CDS             complement(3309..>3493)
                     /locus_tag="PKZ53_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40294.1"
                     /translation="ALSPLSSALSPLSSALSPLSSALSPLSSALSPLSSALSPSSSTP
                     SPTSSALSPLSSARCL"
BASE COUNT          892 a          888 c          905 g          808 t
ORIGIN      
        1 gggttgacgt ctgaaatcat ttcggccttg tttgaagatc gccaaaaacg gctctggatt
       61 ggcacccgca aagggttgaa ttatctgtct ttagagtcac caaaccaggg attcaaacga
      121 gttgacaccc tggatggcaa agctgtcact gatattcggg cgattggaca atcggcagat
      181 ggcagcattt tgctgggcag ccagaatatg ctctttcagg taatcaatcg taccgacgcg
      241 ctggcactaa cctcatataa aacacttgaa ggtcaaatcg tcagtctctt cacagatcaa
      301 aagcagaatc tctggattgg tacgattgga gccgggttgt atcaggaacg acctgaccac
      361 accctgaccc actttaccaa tgccactggg ttttcaggca cggtcgcttc tgttttttgc
      421 gaagatcggg acggaaatct gtggattggg tcggccaaaa ttgggctcgt tcggtttgcg
      481 gacaacaaat ttacggcgtt tactcagtca gatggccttt cgggtgaaga cgtatcgtcc
      541 ttgcttgaag accgtgaagg aaatctctgg gtcggaacct atcgggatgg actcaatcaa
      601 ctccaaaacg ggaagtttct tacctacacc accaaagaag ggttgtcaca caacattgtc
      661 tggcaaattc aggaagccca ggatcatagt ttgttcatct gtaccaacca ggggctgact
      721 catctcacca acggcaagct cacccgtttc gtgacaggtt attttaattc aaccgtttac
      781 tgtgctgtac aggatcaatc tggcaactgg tgggctggca cgcaaagtaa cggactcgtt
      841 caatttcatc cagatttacc caaccgcccc ccactggcga ccttcaccac ccaaaccggg
      901 ttgcccagca atcaggttcg ggcgctgtct gaagacaccc agggaaatct ctggctgggt
      961 acaccagcgg gactgtgccg caaaagtgga gcgacgatta caaccttcac cactgaaaat
     1021 ggccttccct taaatgtcat cctggcaatt acctgtgcca aagacgggag tgtgtgggtt
     1081 ggaaccgatg ggggcgggct cacccggttt caaaatggcc agtttacgac tttcacacga
     1141 acagaaggtc tcccaagcaa cgtcatttta tcgctgtatg aagacgtaga cggtatcctc
     1201 tgggtgatta ccaatgccgg gttgtgctat gtgcaggaag gcaaactccg aacgttgagt
     1261 cagcttcctg gcaatcaacc cgaaacactc catcaaataa tcgaagacac ccagggaaat
     1321 ttttggattg ggagtggcga tggcattctt cgagtcaatc gcaaagcact gctccagttt
     1381 gcccagggac aaatgccagc cggagaagca gcgctgttta cacagtttga tggaatgaaa
     1441 accgatgaaa ccaatgttcc cgccccagcc ctgagggcct ctgatggaac actctggttt
     1501 ccgacgttgt gtggccttgc catcattgat ccagtcagta tccccatcaa tccagttccg
     1561 ccaaccgtcg tgatggaaag tgtcacggcc aatggggaag ccctgcgtcc aggcccagat
     1621 aaactggcac tcgcttcgtt tcagaatact tttgaagtga cctgctcggt cttgagtttt
     1681 tcagcccccc aacgaaatcg catcaagtac cgcctggctg agttccaatc acagtggagt
     1741 gaagtgcaag gaaaacgcac gctttctttt ttcaatattc cttctggaaa atacacactc
     1801 gaaatcctgg cctgtaacaa tgacggcgtg tggagcatgc ttcccaatcg cctggccgtt
     1861 cacattcaga cctctccgtg gcgttcgccc tgggcaattc tggtttacag tggagttgtg
     1921 attgggctga ttttcagcag tgcccggctc cgcaccgggt atctggttcg acaaaaccaa
     1981 agactgcaag ttcaagttgg cgaacgaacc cgaaaactgg ccgaaaccgc atttcactta
     2041 tggcagcagc aacaggaact ggaacaccaa aaaaatgtgc tggccgaagc caacctgaaa
     2101 ctccaggaaa tagaccggct taaagcaagc tttaccgcca tgctggctca tgatttaaaa
     2161 tctccgctca gtgtggtcaa aaccgccatc gaactgttgc agatggaatc ttcgattgcc
     2221 tccggtccgc tcgcgtctct gctccaggcg tcagaccgga gcctggataa agtcctgacc
     2281 ctgatttcag aaatgctcga agtgtcacga gccgaagccc aggaaatgaa actgtcctgt
     2341 gcgccactgg aaaccgagtc tttcttgcgc caatgtgccg aggatgtccg tgtcagtgcc
     2401 cgggcgcgcc ggattaccct cgaaatcaag cttgaaccag gattaccggc aatttcggct
     2461 gatcggggaa aacttgagcg cgtgtttgcc aatctgctgt ccaatgccct gaaatttacc
     2521 ccggatggcg gcacaatttc gattgaagga tgggtcgaaa ccggcactgg agttgaaacc
     2581 ggtcagcagt tgttgaaagt ttcaatcacc gatacgggcg aaggaattcc ggctgaagac
     2641 atcccctatt tgtttgagcc atatcggcag tcaaaatcaa ccaaaaagca gttgggagtt
     2701 ggccttggac tgacaattgt gagacgcatc gtggccgcgc acggaggcaa tatctcggtt
     2761 cacagccagt tgggtgttgg aacgtgtttt acggtgctcc tgccgacgct cccacctgaa
     2821 gcatcgtctc tcgccgaagt atcgccgtta tcagtggcga ccgaagataa atcgtttgtt
     2881 tccgccgtgc ccaatagccc tgatgccaat tcgatcccgc ccatttcgcc acgggttctg
     2941 attgcggaag acaatgtcat cagccaaaga attctggccg cccaggtgaa aaaacttggc
     3001 tatcaggtcg aagtcgtcaa caacgggctc gaagccgtta acgccgttga aacatcgcga
     3061 tttgatgcca ttttgatgga ttgctatatg cctgaaatgg atggatttac agcgacgcag
     3121 gccattcgtc gcaagaaaac ccatcgttct aaaatcagaa tcattgcact gacggctgaa
     3181 acaactgttg aaacagtgga aaaatgtctc caaatcggca tggatgactt tttaggaaaa
     3241 ccctttacct ttgaggattt aaaggctttg ttacaacggt gggtgccaat tatcgcgacc
     3301 gaagagggct aaagacaacg ggctgaagac aatgggctca gggctgaaga cgttgggctt
     3361 ggggttgaag acgacgggct cagggctgaa gacaatgggc tcagggctga agacaatggg
     3421 ctcagggctg aagacaatgg gctcagggct gaagacaatg ggctcagggc tgaagacaat
     3481 gggctcaggg ctg
//
LOCUS       DAOQDO010000378         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20095_length_3490_cov_3.070451, whole genome shotgun sequence.
ACCESSION   DAOQDO010000378 DAOQDO010000000
VERSION     DAOQDO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20095_length_3490_cov_3.070451"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKZ53_07390"
     CDS             <1..607
                     /locus_tag="PKZ53_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40295.1"
                     /translation="LTFAWLEKSYQAFWLMLAGNIVSSMVIWLSFPLTAAPEPGFILF
                     FLLIFGITSVAAQRFLDTMGFQERWWFNRGTIALFVIGLYILSFITFAAAYQNLERGA
                     IGWYWGFKVVAMIGSLTVSLFISIFYEEWVISSGSETQHLSPIRLDRVVKANLVTMGL
                     ILTVLAVNILPQRFGYSFVLVKLMKYPQSNVSASAQDLYKR"
     gene            complement(586..891)
                     /locus_tag="PKZ53_07395"
     CDS             complement(586..891)
                     /locus_tag="PKZ53_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40296.1"
                     /translation="MKMYILVKESVPVGYALVAVAHASLAAYLKFQDSPEVSEWLAGP
                     FYKTICKVTDAEFEQAKTVLDHVIITESKLDGAEVAIAFKPRAEWPKPFKFYRLYKS"
     gene            1314..2069
                     /locus_tag="PKZ53_07400"
     CDS             1314..2069
                     /locus_tag="PKZ53_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40297.1"
                     /translation="MAQVSPKHFYKVNMRTFSLSEILHYQPNLNGFLSWFLNAVGIQV
                     LPTFDLKVPLPVRADSFFCEHGELSHQGKEIISPLVESFERLGFAVMGYEKNTGIDPN
                     CLDAAGCHLLHQNSKYTASVLFLHSVEAPEPHFVTLNISCLDSEHHSFSVHNHPLAFD
                     FFAAKTLKSTCTVVQTRSPFELFREFESKVQHRLNFSTLQFIRNPHHFAQLTEARSET
                     VLNEGLRLGRFIPMTEIEVQELKRKHSWRYAQA"
     gene            2220..>3490
                     /locus_tag="PKZ53_07405"
     CDS             2220..>3490
                     /locus_tag="PKZ53_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HNJ40298.1"
                     /translation="MVPLRTGIGALMCFVFLFLVTSLVKAEDWKPIEPAHLALKAPLV
                     DPGADAEAIFWEVRVNDSETDPTFDHYIRIKVFTERGVEKQSKIDIIYPDKIRIRGIS
                     GRTIQPDGSIQELKKDGIFERTVIKANKTRFKAKSFTLPGVKIGSIIEYKWSESRPSG
                     FADNVPLRFQRDIPCQSVSYLIKPWSDRNSNIKMRSIVFNGPEASFDKGNFGFTRISM
                     KNRPAVIEEPMMPPDDEIENWMLLYYTELGDYTPEKYWKDMSRRLYDATRGYIKPNDE
                     VKQLAASLVAGITTPEQKIEKLFWYCRTKIKNTTYEDLPNEDKLKENKSPGNTLKNGY
                     GDAADINYLFGAMCAAAGLDPRVALVPDRGQKFFDMSHTLFFMEQIVIAVRVGANWQF
                     LDPGTSYVPVGMRKWQGEGTQALILDEKESK"
BASE COUNT          919 a          811 c          850 g          910 t
ORIGIN      
        1 actcacgttt gcctggcttg aaaagtctta tcaggcgttt tggctcatgc tggctggaaa
       61 catagtgagt tcaatggtga tttggctgtc gtttccgctg acggctgccc ctgagcctgg
      121 ctttatcctt ttcttcctct tgatttttgg catcacctcc gtagctgccc aaaggttttt
      181 ggacacaatg ggttttcagg aacgctggtg gttcaatcgg ggcactatcg ccttgtttgt
      241 gattgggttg tacattcttt catttattac ttttgcggct gcctaccaga atctggagcg
      301 cggagctatt ggctggtatt gggggttcaa agtcgtggcc atgattgggt cgctgactgt
      361 aagcctcttt atatcaattt tttatgaaga atgggttatc tcctcaggtt cagaaacaca
      421 acacctgagc ccgattcgac tggatcgggt cgtcaaggcc aatctggtga ccatgggatt
      481 gattttgacc gtactggcgg tcaatatcct gccgcaacgg tttggttatt cctttgtact
      541 ggtcaaactg atgaagtatc cccaaagtaa tgtttccgca tcagctcaag atttatacaa
      601 acgatagaac ttgaatggtt tgggccattc agcccgaggc ttgaaggcta tcgccacttc
      661 ggcaccgtca agctttgatt cggtgatgat gacgtgatcc aggacggttt tggcctgttc
      721 aaattcggca tctgtgacct tgcagatagt tttgtaaaag ggacccgcga gccactcgga
      781 aacttcaggc gagtcctgaa atttcaaata tgctgccagc gaggcatggg ctacggccac
      841 aagcgcgtac ccaaccggaa ccgattcctt gaccagaata tacattttca tgatgcttca
      901 atcctcgggc tcagggctga agacaacggg cttggggctg aagattcgca agctcagggc
      961 aaaggaaggg atgagggatg aaggatgagg gatgaaataa aaccagttct tcaacccgac
     1021 gtcttcagcc ccaagcccga tatcttcagc ccgatatctt cagcccgact tcttcaaccc
     1081 aacttcttcc gccccaaaat agtgacttgg cgaaatcaac aaagttgagt atagtgggtt
     1141 tatccaacaa cttggaagaa ctttgcaggt tacagccacc taacctctct cagggagatg
     1201 attcaactgg tttttgtggg gaagttcgtc agtgtatctg gtattgaagg attcgtgtcg
     1261 cgcaatcctg agaaacactt gaacgcccac gttgaatata gaaagtggag tttatggctc
     1321 aagtttctcc gaaacatttt tacaaggtga acatgcgcac gttttcgctg tctgaaattc
     1381 ttcattatca gcccaacctc aatgggttct tgtcgtggtt tttgaatgct gtgggaatcc
     1441 aggttttacc gacttttgac ttgaaagtac cactacctgt gcgggcggat agtttctttt
     1501 gcgagcatgg ggagctttcg catcaaggga aggaaataat ttctccattg gttgaaagtt
     1561 tcgaacggct tggctttgcg gtgatggggt atgaaaaaaa cacggggatt gatccgaatt
     1621 gcctcgatgc cgccggttgt cacttgcttc atcaaaacag caagtacacc gcaagtgtgc
     1681 tttttcttca ctcggttgaa gcaccagaac ctcactttgt gacactgaat atcagttgcc
     1741 ttgattcaga acatcactca ttttcggtgc ataaccaccc actggctttt gacttttttg
     1801 ccgcgaaaac actgaaatcc acctgtacag tggttcagac acgttccccg tttgaacttt
     1861 ttcgagaatt tgaatcaaag gttcagcacc gactgaattt ctcaaccctt caattcattc
     1921 gcaacccaca tcattttgct caactgactg aagcccggag cgaaacagta ctcaacgaag
     1981 ggttacgcct tggccggttt attccaatga ccgaaatcga ggtccaggaa ctcaagcgga
     2041 aacatagctg gcggtatgct caggcgtaat cgaacaatac aggacgtgtg acgcaccaaa
     2101 aaattgtaat aaggctcaaa attttgcatt cagccttttg acatactcac aactcacaac
     2161 aagaagttac ggccactcgc ccacagcgcg aacctacaat gttagaaaga ggaatggata
     2221 tggtaccact tcgaacaggc atcggagccc tgatgtgctt cgtgtttctt ttcctggtta
     2281 cttcgctggt caaagcggaa gattggaaac caattgaacc tgcccacctg gcgctcaagg
     2341 ccccgcttgt tgatccaggc gcggatgctg aagcgatctt ttgggaggtt cgagtcaatg
     2401 attccgaaac tgacccaacc tttgaccact acatccggat taaggtcttt accgagcgcg
     2461 gggttgaaaa acaatccaaa attgacatca tctaccctga caaaatcagg atccgtggca
     2521 tttcaggaag aacaattcag cctgatggct caattcagga actcaaaaaa gacggaattt
     2581 ttgagcgaac cgttatcaaa gcgaataaaa cccggtttaa agccaaatca tttaccctac
     2641 caggtgtcaa aattggttcg attatcgagt ataaatggag tgagagtcgg cccagtggct
     2701 ttgccgataa tgttccgttg cgctttcagc gggatattcc gtgtcagagt gtcagctatc
     2761 tgatcaaacc gtggagtgac cgaaattcca atatcaaaat gcgctcgatt gtgtttaatg
     2821 ggccggaagc cagttttgat aaaggaaact ttggctttac ccggatttcg atgaaaaacc
     2881 gcccggcagt gattgaagaa ccgatgatgc cgccagatga tgaaattgaa aactggatgt
     2941 tgctctacta cacagagtta ggcgactaca ccccagaaaa atactggaaa gatatgagcc
     3001 gccggctcta tgacgcgacg cgcgggtata tcaagcccaa cgatgaggtc aaacaactcg
     3061 ccgcttcgct ggtggctggc atcaccacac cggaacaaaa aattgagaag ttgttctggt
     3121 actgtcggac caaaatcaaa aatacaacct atgaagacct gcccaatgag gacaagctca
     3181 aagaaaacaa atcgccaggc aatactttaa aaaatggata tggcgatgcg gcagacatta
     3241 actatctctt tggtgcaatg tgcgcagcag ccgggctcga cccccgggtg gcgctggtgc
     3301 cagatcgagg ccagaagttt tttgatatga gccataccct gttctttatg gagcagatcg
     3361 tgattgccgt ccgggttgga gcaaactggc aatttctaga ccctggcacc agctatgttc
     3421 cggtcggaat gcgaaagtgg cagggagaag ggacccaggc gctgattttg gatgaaaaag
     3481 agtcaaagtt
//
LOCUS       DAOQDO010000379         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20132_length_3487_cov_3.168706, whole genome shotgun sequence.
ACCESSION   DAOQDO010000379 DAOQDO010000000
VERSION     DAOQDO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20132_length_3487_cov_3.168706"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKZ53_07410"
     CDS             complement(<1..857)
                     /locus_tag="PKZ53_07410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682251.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNJ40299.1"
                     /translation="MSASNQIGSVTQVIGPVIDVEFGNNYLPPVYQALRVTSDGFEVP
                     EPIDIILEVQQHLGEGRVRCVSMLPTEGIVRGMKAVDTGDQISMPVGKGTLGRVLNVI
                     GEPVDKLGKVPHTKRYPIHRPAPSFEQQSTESVMLETGIKVIDLIQPFVKGGKIGLFG
                     GAGVGKTVLIMELINNIAKGHGGYSVFAGVGERTREGNDLWEEMKESKVIDFDKIENS
                     KVALIYGQMTEPPGARARVALSGLSVAEYFRDEMNQDVLFFVDNIFRFTQAGSEVSAL
                     LGRMPSAVG"
     gene            complement(979..2070)
                     /locus_tag="PKZ53_07415"
     CDS             complement(979..2070)
                     /locus_tag="PKZ53_07415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ40300.1"
                     /translation="MLPSPNELVGRVFNNRYQLTACLSTRKTVSVYRATDLSTHTEIA
                     VKVLHVGVSEADQITRKRFRREAHAMSLVNHPNVIKVFEIADADMGLTFFTMELLIGE
                     SLLDFLCRIGPLSLAQAVEIFTPVCQAIHAAHTVGLVHRDVKPGNVFLHRNGQTEVEE
                     DAVGQVKVLDFGIAKPRWEFRSDNEEANVSGEGTIIGTPEYMSPEQCRGDEVTPQSDI
                     YSLGVMLYRMLTGYPPFDGPPSAILMRHLVAEPPSLRMRRPNLPPIIETLTFRAMAKT
                     PEARYLSALDFANAFTEAVNQTYTPDSDQIYRTVSPPEQHKDAIASQTVVLSSADLEP
                     LSRDSNSGRVSIKQLLRQILPFNSTGETD"
     gene            complement(2279..3199)
                     /locus_tag="PKZ53_07420"
     CDS             complement(2279..3199)
                     /locus_tag="PKZ53_07420"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNJ40301.1"
                     /translation="MFKPPALRPDDTIGIIAPGSPITAEQLAAGVQELERRGFQTRYR
                     EDILAAERYLAGSLERRLTEFYELWEDPRVRAVIAARGGYGCQHLLPFLDVTRLRRHP
                     KILVGYSDLTALQLFLVQDCELVTFHGPMVTKDFAEGPAHYDWDSFERILCHTTPQGV
                     LTSPGTSTLIGGTATGRLVGGCLSLIAALIGTPWQLETQHSILFLEDVAERPFRLDRM
                     LHQMKLAGMFEQVRGILFGEMYDCTIPADAGYQLEDLCFDLIKDIGVPTLFGWQSGHS
                     PMKNRTLPLGIQVKLDADRQEVELLEAAVK"
BASE COUNT          835 a          895 c          927 g          830 t
ORIGIN      
        1 tatcccacgg ctgaaggcat ccggcccaac agtgccgaca cttccgaacc ggcctgggtg
       61 aagcggaaga tgttgtccac gaagaacaac acgtcctggt tcatttcatc gcggaaatat
      121 tcagcgactg acagacctga gagcgccacg cgggcacggg ctccgggcgg ttcggtcatc
      181 tgaccataga tgagcgccac ttttgagttt tcgattttat cgaagtcaat gaccttggat
      241 tctttcattt cttcccagag gtcgttgcct tcacgggttc gctcgccaac accggcaaat
      301 accgagtatc caccgtggcc tttggcgatg ttgttgatga gttccatgat gagcacggtt
      361 ttgcccacgc ccgcgccccc gaacagtccg atttttccac ccttcacaaa cggctggatc
      421 aaatcaatga ccttgatgcc ggtttcgagc atcacagatt cagtggattg ttgttcaaac
      481 gatggtgccg gacggtgaat cgggtagcgc ttggtgtggg ggactttgcc cagtttatcc
      541 accggctcgc cgatgacatt gagcacccgg cccagggtgc ctttgcccac tggcattgaa
      601 atctggtcac cggtgtcaac cgctttcata ccgcgcacga tgccttcggt cggcagcatc
      661 gacacacagc ggacacggcc ttcacccagg tgttgctgaa cttcgagaat gatatcaatg
      721 ggttcgggaa cttcaaaccc gtcactcgtg acccgcaggg cctggtacac gggtggcagg
      781 tagttgttgc caaactcaac gtcaatcaca ggcccgatga cctgtgtgac actcccaatt
      841 tgattgcttg cactcatggc gcggaagact ccttacaaga tagaaaaata attgcagatt
      901 ggttgatgaa tgatagatga aaaggtacag gtaaactgaa aaggggaaac tctagcatag
      961 agacgggatg agactcaatc agtctgtttc cccagtcgag ttgaatggca gaatttgtcg
     1021 aagtaattgc ttgattgaaa cccgtccaga gttggaatcg cgtgagagtg gttctaaatc
     1081 ggctgaactc agaacgactg tctgagaggc aatcgcgtct ttgtgttgtt cgggtgggga
     1141 gactgtccgg taaatctggt ctgaatctgg ggtatatgtt tgattgacgg cctcagtaaa
     1201 cgcattggca aaatcgagcg ctgaaaggta gcgggcttcg ggggttttgg ccattgcccg
     1261 gaaggtgagc gtttcaatga tcggcgggag attggggcgg cgcatccgta atgacggcgg
     1321 ttcggcgacc aggtgccgca tcaggatggc gcttggtggg ccatcaaatg gcggataccc
     1381 ggtcaacatg cgatagagca tcacacccag gctatagata tcactttgcg gtgtgacctc
     1441 atcgccccgg cattgttccg ggctcatata ttctggcgtg ccaataatgg tgccttcgcc
     1501 ggaaacattg gcctcttcat tgtcagaccg aaactcccag cgtggtttgg caataccaaa
     1561 atcaagcact ttcacctggc caactgcgtc ttcctccact tcggtctgcc cattccgatg
     1621 caaaaagaca tttcccggct tgacgtcacg atggaccagc ccaacggtat gcgcggcatg
     1681 gattgcctgg cacactggtg taaagatttc aactgcttga gcgagcgata acgggccgat
     1741 tcgacacaga aagtcaagca ggctctcgcc aatgagcaat tccatggtga aaaacgtgag
     1801 gcccatgtca gcatcagcga tttcaaacac tttgatcaca tttgggtggt taaccagcga
     1861 catggcgtgg gcttcacgcc gaaaccgttt ccgtgtgatt tgatcagcct cagatacccc
     1921 gacgtggagc actttaaccg cgatttccgt atgggttgac aggtcagttg cacgatagac
     1981 agacactgtc ttgcgcgtgc tcaggcacgc ggttaactga tagcggttat taaagacacg
     2041 tcccacaagt tcatttggcg agggaagcat gaaaacctgc ctctttgggt tccgggttcc
     2101 gggtttttta agctggggta actgacaacc gagaaaacac cttactttac actgatttgg
     2161 cgaaagagcg aaatagcaaa agaaaagcga atagcgagtc gcgagtcgtc aaacccagaa
     2221 gagtgaagtc gtgaagtcgt gagttgtcaa ccaggcgctt tgtcattgtg tcagcttgtc
     2281 acttgacagc ggcttccagg agttccactt cctggcggtc agcatcaagt ttgacctgaa
     2341 tacccagggg caaggtacga tttttcattg ggctatgccc cgattgccag ccaaagaggg
     2401 tcggtacgcc gatgtctttg atcaggtcaa agcatagatc ctcaagctga tagccagcgt
     2461 cggcaggaat ggtgcaatca tacatttccc caaacaaaat gccgcgaacc tgctcaaaca
     2521 ttccagccag tttcatctgg tgcagcattc ggtcgagccg gaacggtcgt tcggccacat
     2581 cttccagaaa caaaattgaa tgttgtgttt caagctgcca gggcgtccca atcaaggccg
     2641 cgatgagtga caagcacccg ccaaccagcc gtccagttgc cgtgccgcca atcaaggttg
     2701 atgttccagg tgaggtcaac actccctgag gggtggtgtg gcacagtatc cgctcaaatg
     2761 agtcccaatc ataatgcgcc gggccctcag cgaaatcttt ggtcaccatc gggccatgaa
     2821 aggttaccag ttcacaatct tgaaccagaa aaagctgcaa ggcagtcagg tcactatatc
     2881 caaccagaat ttttgggtgc ctgcgaaggc gcgttacatc cagaaacggc agcaggtgct
     2941 ggcatccata tccaccccgg gccgcaatca ccgccctcac tcgcggatct tcccagagtt
     3001 cataaaactc ggtgagtcgt cgttcaagcg acccggccag ataccgttcg gcggccagga
     3061 tgtcttcccg gtagcgagtc tgaaacccac gtcgttcaag ttcctgtacc ccggcggcaa
     3121 gctgctcagc cgtaattggg ctgccagggg caataattcc aatggtgtcg tcaggtcgaa
     3181 gggcaggtgg cttgaacata ggaagagaag agtctccaga attctggttg gcgctatcag
     3241 gtgagcattg catgatcgtg tgggtttgga atacaaacaa accacctgct caccgttgat
     3301 tcccgataat agccagagtt tctcagaaaa aggctttttc tgaaaatcta tatattcttc
     3361 attcttcatt cttcattctt tattcctcat gctgactgtt gaaccacttt ttggattgtg
     3421 taatcggatg caggcattgt cttctgtcct gtcactggct gaagatttga aacagccagt
     3481 aacggtg
//
LOCUS       DAOQDO010000380         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20134_length_3487_cov_2.707751, whole genome shotgun sequence.
ACCESSION   DAOQDO010000380 DAOQDO010000000
VERSION     DAOQDO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20134_length_3487_cov_2.707751"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3178)
                     /locus_tag="PKZ53_07425"
     CDS             complement(<1..3178)
                     /locus_tag="PKZ53_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ40302.1"
                     /translation="MDIFDFRDQIIGDYSSYVKSFIQIKDLRIKAYVNELLHQGELWP
                     EPLVQLSPTFAPGKSIEELVSEQTLHPECRRIFRRNKIPAATTLSGLPLQLHFHQQRA
                     VEVAQSHQHYVLTTGTGSGKSLSYIIPIVDYCLRHPKRNQIKAIIVYPMNALANSQAG
                     ELKKFLAFGYPDNKGPVTFERYTGQETQEERARIQEHPPDILLTNYVMLELLLTRRRE
                     QALISAAKGLKFLVLDELHTYRGRQGADVALLIRRLRDRLEATSLQCIGTSATLSSHG
                     DYQEQQAEIARRASVFFGVLIKPEHIIGETLRRSTQEPDLTSATTIQELTHCVMQSPM
                     TPAKSFQEFTQNPLSQWIESTFGVRREESSGRLVRAIPQTISGAEGASQVLSQLTGVA
                     AEQCAQAIRAGLIAGNICDRNPATGFPPFAFRLHQFISRGDTIFSTIEPEADRYLTSQ
                     GQQYVPGDRGRVLLPLAFCRECGQEYAIVRRVKHPRTGEIAYLPRTLSDRGEDGGEEA
                     GYLYVNQTKPWPTSPTEVLERLPDDWLEEMRGTVRIKNHRRPQLPRTVQILPTAVEGE
                     GGVEACFLFAPFSFCLYCGVAYAARQYSDFAKLTTLGSEGRSTATTILSLAAIRALRR
                     ESDIVPEAKKLLSFTDNRQDASLQAGHFNDFVEIGLLRTALFLAVQKAGSSGIEHDVL
                     TQKVFEAMQLPLELYAADQTVRFQALVETQRALRQVLGYRLYCDLKRGWRITSPNLEQ
                     CGLLRIEYLSLEDVCAAQDIWAQCHPVLAKATPETRVKVATVLLDLMRRGLAIKVDYL
                     NPEPQERILQQSSQRLRAPWAIDENEANRMEFASVLFPRASRQGKDTREHLYLSGRSR
                     FGLYLSRASTFGNQAGKLGIADREEVIRDLLAGLQQAGIVEQVVAGTDTEPGGYQVTA
                     SSLRWVAGDGSQPFRDPTYVPEESELGTRANPFFVRFYQIMAQDLLNLEAREHTAQVT
                     SEKREEREEKFRRGALPVLYCSPTMELGIDIADLTAVNMRNIPPTPANYAQRSGRAGR
                     SGQPAFIFTYCSTGSSHDQYFFKRPDRMV"
BASE COUNT          831 a          928 c          850 g          878 t
ORIGIN      
        1 ccaccattcg gtcaggacgt ttaaaaaagt actggtcgtg tgaactacca gttgagcaat
       61 aagtaaagat aaacgctggt tgtccactgc gtccggcacg accgcttcgc tgggcatagt
      121 tggccggcgt tggtggaata ttgcgcatgt tgaccgcagt caaatcggca atatcaatgc
      181 ccagttccat ggtcggtgaa caatacagca ctggtagtgc gccacggcga aacttctctt
      241 cgcgttcttc gcgcttttca ctcgtgacct gcgccgtgtg ttcccgtgcc tccaggttga
      301 gcagatcctg ggccatgatt tggtaaaacc ggacaaaaaa tggattggca cgagtcccaa
      361 gctcagattc ttcaggaaca taggttgggt cgcggaatgg ctgactgcca tcacctgcca
      421 cccatcggag cgaagaggct gtaacctggt atcctccggg ctcagtatca gttccggcaa
      481 ccacctgctc gacaattccc gcctgttgca atccggccag caaatcacga atgacttcct
      541 cccggtcagc gatccccagt tttccagcct gatttccaaa tgtggatgcc cggctcaaat
      601 acaacccaaa gcggctcctc cctgagaggt agagatgttc cctggtatct ttcccttgcc
      661 gtgaagctcg cggaaacagc actgatgcaa attccatccg gttggcttcg ttttcgtcaa
      721 ttgcccatgg ggcccgaagc cgctggctgc tctgctgcag gattcgttcc tggggttccg
      781 gattcaaata atctaccttg attgccagtc cacgtcgcat caaatccagc aataccgtgg
      841 ccacctttac tctggtttca ggggttgctt tcgccagcac aggatgacac tgagcccaaa
      901 tatcttgagc tgcgcagaca tcttccagtg acaggtactc aattcgcagc aaaccgcatt
      961 gttccagatt cggtgaggtg attcgccacc cccgctttaa atcacaatac agccgatacc
     1021 caaggacctg gcgcagcgcc cgctgggttt caaccagtgc ctggaaccga accgtttgat
     1081 cagcggcata tagttcaagc ggcaactgca tcgcctcaaa cactttttgg gtcaacacat
     1141 cgtgttcaat tccggatgac cccgcttttt ggacggccag aaacagagcg gtccgtaaca
     1201 atccgatttc gacaaaatca ttaaaatgcc cggcctgcag cgaagcatct tgccggttat
     1261 cagtaaaact tagaagtttt ttggcttcgg gaacaatgtc ggattcccgc cgcagggccc
     1321 gaattgccgc cagactcaaa atcgtggttg ctgtgctgcg accttcggac ccaagtgtgg
     1381 tcagtttggc aaaatccgaa tactggcgag ccgcataggc cacaccacag tacagacaaa
     1441 acgaaaaggg cgcgaacagg aaacaggctt caaccccacc ttctccctca accgcggtag
     1501 ggagaatctg cacggttctc ggaagttggg gccgacgatg gtttttgatt ctgactgtac
     1561 cgcgcatctc ttccaaccag tcatcgggta atcgttcaag cacttcggtt ggactggttg
     1621 gccagggttt tgtctgattg acatagagat atcccgcttc ctcaccacca tcttcccccc
     1681 ggtcggaaag cgttcgagga agatatgcaa tctcacctgt ccgtggatgt ttcacccgtc
     1741 gaacaatcgc atattcctgc ccacattccc gacaaaaagc cagcggcaac aacacccggc
     1801 ctcgatctcc cggcacatac tgttgtccct gtgaagtcag gtagcgatct gcttccggtt
     1861 caatcgtgct gaagatggta tcgccacgac tgatgaactg gtgaagacga aaggcaaagg
     1921 gcggaaaccc cgtcgccggg tttcggtcac agatatttcc cgcaatcaga cctgcgcgaa
     1981 tggcctgtgc gcattgttca gccgcaaccc cagtcagttg actcaacacc tggcttgccc
     2041 cttctgcacc ggaaatggtt tgaggaattg ctcgaaccag tcgcccagat gattcttcac
     2101 gtcgaacccc aaaggtgctt tcaatccatt gtgaaagtgg attttgggtg aattcctgaa
     2161 aagatttggc gggcgtcatt ggtgactgca tcacacagtg agtcaattcc tgaatggtgg
     2221 ttgccgacgt cagatctggt tcctgggttg aacgacgcag cgtttcaccg atgatgtgtt
     2281 cgggcttaat cagcacccca aaaaagactg acgcccggcg ggcaatttcg gcctgttgtt
     2341 cttgataatc accgtggctg gaaagtgtgg ccgaagtccc aatacactga agtgatgttg
     2401 cttccagacg atcccgcaac cgacgaatga gcagtgcaac atcagctccc tgccgtccac
     2461 gataggtatg tagttcatct aaaaccagga acttcaatcc tttagcggcg gaaattagtg
     2521 cttgttcacg ccgcctggta aggagtaatt ccaacatgac gtagtttgtt agcaggatat
     2581 ccggtggatg ttcctgaatt cgggcccgtt cttcctgcgt ttcctgccca gtgtagcgtt
     2641 caaacgtcac gggtcccttg ttgtccggat acccaaacgc gaggaatttt ttcaactcac
     2701 ctgcctgact gttggccagc gcattcatcg gatacacaat gatcgcttta atctggtttc
     2761 gcttcggatg gcgtaaacaa tagtcaacaa ttgggataat gtaactcaaa cttttccctg
     2821 atccagtacc cgttgttaga acatagtgct gatggctttg agcaacttca acggcccgtt
     2881 gctggtgaaa atgaagttgg agtggcagtc ctgacaaggt tgttgcggca ggaattttgt
     2941 ttcggcgaaa aattcgccgg cattccggat gcaatgtttg ttctgatacc agttcttcaa
     3001 tggacttgcc cggagcgaat gttggactga gttgcactaa gggctcaggc cacagttctc
     3061 cctggtgtaa caactcgttc acatacgctt tgatccttaa atctttgatt tgaatgaagc
     3121 tttttacata ggaactatag tccccaatga tctgatcacg gaagtcaaaa atgtccatct
     3181 gttcgggctc cttcagtata ttgtgcttct tggattttgt atcaatcaga atacttactt
     3241 cttcaccgga aagctatcgt ttcctttatg cgggaatatg aaagaactaa attggagtcg
     3301 aagaccatca ggtgagaaaa cgtgctgaag aacccctttt ccgtaagtca tcagaaagaa
     3361 caaatcccca ctttaagcag tcttcgcacg tgcatttttc gtctagttca gaattgcacg
     3421 aaaacctggc tcgacaacga aagtgtcgta tttgggtagt acctcgttcg ccaactgatt
     3481 cggttaa
//
LOCUS       DAOQDO010000381         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20135_length_3487_cov_2.693182, whole genome shotgun sequence.
ACCESSION   DAOQDO010000381 DAOQDO010000000
VERSION     DAOQDO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20135_length_3487_cov_2.693182"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKZ53_07430"
     CDS             <1..193
                     /locus_tag="PKZ53_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNJ40303.1"
                     /translation="APSLGGVESLITRPVQTSHSGLSPEDRQKAGISNGLIRVSVGLE
                     ATEDLIEDFSDALKKIREA"
     gene            complement(333..830)
                     /locus_tag="PKZ53_07435"
     CDS             complement(333..830)
                     /locus_tag="PKZ53_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40304.1"
                     /translation="MLKKLIFAMTFVLAASLSTLAQEPPENEVVAGYTYLRGDELNGN
                     LNGFNFSYTRYLNDNFGFTSELGANFDSGVNTFTYLFGPQLAANRGGRVSPFAHILVG
                     GGRVSFEDIGASSGFAVALGGGVDVKLNDKVSIRPIQVDYLPIRFNGGTIHNGRVGAG
                     INFRF"
     gene            1072..1983
                     /locus_tag="PKZ53_07440"
     CDS             1072..1983
                     /locus_tag="PKZ53_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNJ40305.1"
                     /translation="MELNYHHLFLFWTVAREGSVIQASRTLGLSQPTLSAQLRSLEVT
                     IGKPLFARKGKHQTLTETGNIVFEYATEIFALGKNLADTLQGLPETIASTIRLGISEG
                     FSKLLGCKFIDHAQQQMPGLKIDCCTLREEELQTQLEGNELDVVLSDHPLTLKNVRPQ
                     NHLLLESDLMVFGTPDLISKWNSNLPESLNQSPFFMPAPHFQIRQSLEAWLTTHRVTP
                     VVVGEYTDSALIKLLGSTGRGFFVSPALIADDLRSMYHVSGFQKLDGYVSQVFAVTVE
                     NRPRHTGFLASLQSAASTLQEKTIESV"
     gene            complement(2014..>3487)
                     /locus_tag="PKZ53_07445"
     CDS             complement(2014..>3487)
                     /locus_tag="PKZ53_07445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ40306.1"
                     /translation="RRAGAALLDTRAGTAAGAALFDPAKELKDTSLKPLMIERITVNG
                     QALEYSKDPVVLTYDRDNVVFEYALLSYFRETDTTFQVQLIGYDKKPTAWTTDAKKEY
                     TNLEPNRYTFQVWGKDYAGNVSGPVSFSFEIKPAPWNTWYAYVFYLFVIIGGMYLAVQ
                     TRIKTLQNRAEALERAVQERTAEVVRQKDELSAQNIEIIRQRDEITEKNQELELRSQE
                     IQEKNTKIIDSILYAERIQQATLPYRERIDQSLREYFILFYPKDIVSGDFYWFHYADG
                     ISIIACADCTGHGVPGAFMSMIGNNLLNQIVIENRIHDPALILETMHGGIRRALKQDS
                     AETESQDGMDVALCRIERETGQVVFAGAKRPLYLIDEQGEFIEIKGDTKSIGGKQKEA
                     RRTFTNHEIQIGKGVTLYMATDGYGDQSGEISPKFGTRRFKEFLKSISYLSMEDQYVA
                     LIHELKDHQGAEPQRDDITVIGIRIPGKKRKASEIVKEQI"
BASE COUNT          880 a          888 c          799 g          920 t
ORIGIN      
        1 ggcgccgagc ctgggtggag ttgaatcact gattacccgg cctgtccaaa catctcattc
       61 agggctatca cccgaagatc ggcaaaaagc tggtattagc aacgggttga ttcgtgtctc
      121 agtcggtctg gaagcaacgg aagacctgat tgaagacttt tcggacgcct taaagaaaat
      181 tcgggaagcc tgaaaaacca gggctcaggg cttggggctg aaagactcgc aagctcaggg
      241 ctgaagataa taggttcaat accctgagcc catcgtcttc agcccgtctt cttcagcccg
      301 tcttcttcag cccgatattc ttcagccctg gtttagaatc ggaagtttat accagcaccg
      361 acgcggccat tgtggatcgt gcctccatta aacctgatgg gaaggtaatc aacctggatg
      421 ggccgaatgg atactttgtc attgagtttt acatccaccc ctccgccaag agcgacggca
      481 aatccactgc tggcgccaat atcctcaaaa ctgactcgcc cgcctccgac caatatatgg
      541 gcaaacggtg aaactcttcc accccgattg gctgccagtt gaggaccaaa caggtaggtg
      601 aaagtgttga cgcccgaatc aaaattcgcc ccgagttctg aagtaaaccc aaaattgtca
      661 ttcagatacc gggtatagga aaaattgaac ccgttcaaat tcccatttaa ctcatcacct
      721 cgcaagtagg tgtatccggc cacgacttca ttttcaggcg gctcttgcgc cagggtcgaa
      781 agtgaagcgg ccagcacgaa agtcatcgca aagatcaatt ttttcaacat atcggttgtt
      841 ctccttttta ttctttcagt tacctgtgaa atgaaaattg aaccagcact cgtgatatct
      901 gatgtgtgtt gggttagtcg tctcctgact tctttggttc agtgttgctc ataaacgcat
      961 actctataga taggttctaa tattttccgg gatgcttgca aaatttgtta aattttctat
     1021 aatatattta acaattcccc accgccccat cagtcccgag gacatttcca gatggaatta
     1081 aactaccacc acttgttttt gttttggaca gttgcccggg aaggaagtgt catccaggcc
     1141 agtcggacgc ttgggctaag ccagccaacc ctcagtgcac aacttcggtc tctggaagtg
     1201 accattggaa aaccgctgtt tgcccgcaaa ggtaaacatc aaacgctgac cgaaaccggc
     1261 aatattgtct ttgaatatgc gaccgaaatt tttgcgcttg ggaaaaacct cgcagataca
     1321 ttgcaaggat tgcccgagac aatagcctca acgattcggc ttgggatcag tgaagggttt
     1381 tcgaaactgc tcggctgcaa atttattgac cacgcccaac agcagatgcc gggtttgaaa
     1441 attgactgtt gcaccttgcg ggaagaagaa ctacaaaccc aactggaagg aaatgaactg
     1501 gatgtcgtgc tcagtgatca tccactcacg ctgaaaaacg tcagaccaca aaaccatttg
     1561 ttgctcgaat cagacctgat ggtctttggg acacccgatc tgatttcaaa gtggaattca
     1621 aacctgccag aatcgctcaa tcagtcacct tttttcatgc cagctcctca ctttcagatt
     1681 cgacaatccc tggaagcctg gctgacaaca catcgggtga cgccggtggt ggtgggtgaa
     1741 tataccgaca gtgccctgat caaactactt ggatcaaccg ggcgagggtt ctttgtttcg
     1801 ccagccctga ttgccgatga tttgcgttcg atgtaccacg tttcagggtt tcaaaaactg
     1861 gatggctatg tttcccaggt gtttgcagtc accgtcgaaa atcggccacg ccacactgga
     1921 tttctggcgt ccctccaatc agccgcctca acactccagg aaaagacaat tgaatcagtg
     1981 tgaatcccct ggctttgatt actcacgact cactcagatc tgctctttta cgatttcgct
     2041 cgccttccgt ttttttccgg gaatgcgaat tccaatcacc gtaatgtcgt cgcgttgcgg
     2101 ttcggcaccc tgatggtcct ttaattcgtg aatcagcgcc acatattgat cttccatact
     2161 gagataggaa attgatttta aaaactcttt aaaccgtcgg gtgccaaatt tgggtgaaat
     2221 ttcacctgat tgatccccat atccatcggt cgccatatag agcgtaaccc ctttgccaat
     2281 ttgaatttca tggttggtaa aggtacggcg ggcttctttt tgtttgccac caattgattt
     2341 ggtatcacct ttgatttcaa taaattcacc ctgctcatca ataagataga gcgggcgttt
     2401 ggcgccagca aagacaacct gtccggtttc gcgctcaatt cgacagagcg ccacatccat
     2461 tccatcctgg gattcagttt cagcggaatc ctgtttgagc gcccgccgaa taccgccgtg
     2521 catggtttcc agaatcagag caggatcatg gattcgattt tcgatcacaa tctggttgag
     2581 taaattatta ccgatcatag acataaaggc ccccggcaca ccgtggccag tacaatcggc
     2641 acaggcaatg atactgattc cgtcggcata gtggaaccag taaaagtcac ccgacacaat
     2701 atcctttgga tagaacaaga tgaagtattc tcgcagggac tggtcaatgc gttcgcgata
     2761 gggcaaggtc gcctgctgga ttcgctcggc atagaggata ctatcaatga tcttggtgtt
     2821 tttttcctgg atttcctgag agcgtaattc taactcctgg ttcttttcgg tgatttcatc
     2881 acgctgacga ataatctcaa tattttgcgc ggaaagttca tccttttggc gaaccacttc
     2941 agccgttcgt tcctgaacgg ctcgttccag ggcttcagcc cggttttgaa gggtcttaat
     3001 tcgggtttgt acagctaaat acatcccacc aataatcaca aacaggtaaa aaacataggc
     3061 ataccacgtg ttccacggcg cgggcttgat ctcaaaggaa aatgaaactg ggccggaaac
     3121 atttccggca tagtcttttc cccaaacctg aaatgtgtag cggttaggtt ccaggttggt
     3181 gtattccttt ttcgcatctg tcgtccaggc cgtcggcttt ttgtcatagc cgatcaactg
     3241 gacctggaag gtagtgtctg tttcccgaaa atagctcaac agggcatatt caaacaccac
     3301 attatctcgg tcataggtca gcaccactgg atctttggaa tattccagcg cctgcccgtt
     3361 caccgtaatg cgctcaatca tgagcggttt taacgacgta tcttttaact ctttggccgg
     3421 atcaaacagg gccgcaccgg cagcagtgcc agcccgggta tcgaggagcg cagccccggc
     3481 acgtcgc
//
LOCUS       DAOQDO010000382         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20142_length_3485_cov_3.714286, whole genome shotgun sequence.
ACCESSION   DAOQDO010000382 DAOQDO010000000
VERSION     DAOQDO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20142_length_3485_cov_3.714286"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PKZ53_07450"
     CDS             <1..1167
                     /locus_tag="PKZ53_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ40307.1"
                     /translation="GVERIECTAQDIALSYLPLSHIYERTVMYVFIFAGVSVYFAESV
                     DAIAANLTEVKPTVMTSVPRIFEKILAKIESGVKEAGGLKARLYAWAMENGKEYARMV
                     NDGKPVPFFLKFQRDLGYSLVLSKIKNRIAPRVRFFSSGGAALSEEIAYSFEAMGLPI
                     LQGYGLTETSPVICANSFQENRYGTVGKPMPGVDVKIAEDGEILARGPNIMRGYYKRP
                     EATAEVMKDGWFHTGDIGHFDADGYLRITDRKKDLFKTSGGKYVAPQPIENKLVASHN
                     IQQAIVIGNERKFPAVIIVPDWDVIMELVKTHGIKYKERTELLDHPKILEYFQKEVDR
                     LTPDLASYEKPKKVVLLEQELTIEGGELTPTLKVKRRVVDLKYKDVIDKIYQEK"
     gene            1243..1446
                     /locus_tag="PKZ53_07455"
     CDS             1243..1446
                     /locus_tag="PKZ53_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ40308.1"
                     /translation="MKLKVVIHQAEEGGYWAEVPSIPGCATQGETFEELLNNLYEAVE
                     GCLAVDVSPFLTDERARVMEIAV"
     gene            1443..1649
                     /locus_tag="PKZ53_07460"
     CDS             1443..1649
                     /locus_tag="PKZ53_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002737289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNJ40309.1"
                     /translation="MKSISGKEFAKILERHGWNLLRVQGSHHIYGKAGLPVRISVPIH
                     GNQPLKTGLLRTLLKAAGLSESDL"
     gene            1723..>3485
                     /locus_tag="PKZ53_07465"
     CDS             1723..>3485
                     /locus_tag="PKZ53_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNJ40310.1"
                     /translation="MGAQIAAHLANAGVSVTLLDIVPPSLSPAEEAAGLTLADPKVRN
                     RFAAQAVENMHKMKLAPFFTREQAALIRTGNFEDHLEWLKDADWIIEAIVERLDLKKG
                     LYERIEPFRKPGSLITTNTSGIPIGSLSVGRSDDFRTNFLGTHFFNPPRYMHLVEIIT
                     TPDTNPEAACFSAGLCDKVLGKGLVYANDTPNFIANRIGIFGILQTIKVMTEGGYSIT
                     EVDKMSGPAIGRPKSATFRTADLAGLDTTMLVAGNVYDNAPHDEYRTTFIVPDFFKDM
                     VARKWLGDKTGQGFYKKVQGAGGREILALDYKTMEYVPQPKVSFPSLENAKNISDLGE
                     RLRTLVYGKDRVGEFLWPVISETLLYTARRIPEIANDPIQVDKAMRWGFNWEMGPFEM
                     WDALGVEKTVKKLQAEGREIPQLVQDLLKSGRKSWYESEKGITRVFMPAVKEHVEVPD
                     QSGVIVLKALKDREKVIKKNSGATLVDLGDGVACLEFHTKMNTIGSDTVSMMQQAVKE
                     VEKNFEGLVISNQADNFCAGANLMMVMLAVQEGEWDELNQMIRAFQNANMNLRYSAKP
                     VVVAPFGLTLGGGCEITLHAD"
BASE COUNT          930 a          860 c          905 g          790 t
ORIGIN      
        1 ggggttgaac gcattgaatg tactgctcaa gatattgcgc tttcatacct gccgctctct
       61 catatttatg agcggacggt gatgtatgtc tttatttttg ctggtgtcag cgtgtacttc
      121 gcggaaagtg ttgatgcgat tgccgccaac ctgaccgaag tcaagccaac cgttatgacc
      181 agcgtgcccc ggatttttga aaaaattctg gccaaaatcg aaagtggagt gaaagaagcc
      241 ggtggcttga aagccaggtt gtatgcctgg gcaatggaaa acggcaaaga atatgcccgg
      301 atggtcaatg acggcaaacc agttcctttt ttccttaaat ttcagcgtga tctgggctac
      361 agcctggttc tgagcaaaat taaaaaccgg attgccccac gggtccgctt cttctcctct
      421 ggcggcgcgg cgctctctga agaaatcgcc tacagttttg aagccatggg cctgccgatt
      481 ttgcaaggct acggcctgac cgaaacatcg cccgtgattt gcgccaattc ctttcaggaa
      541 aaccgctatg gcacggtggg caaaccgatg ccgggggttg atgtcaaaat tgcggaagat
      601 ggcgaaatcc tcgcccgtgg cccaaacatt atgagaggct attacaaacg cccagaggct
      661 acggctgaag tcatgaaaga tggctggttt cataccggcg acattggtca ctttgacgcc
      721 gatggctatt tgaggatcac tgaccgcaag aaagatctgt ttaagacctc gggtggtaag
      781 tatgttgctc cgcagccgat tgaaaacaaa ctggttgcct cacacaacat ccagcaggcg
      841 attgtgattg gaaacgagcg caaattcccc gccgtaatca tcgtgcccga ctgggatgtg
      901 attatggaac tcgtcaaaac ccacgggatc aaatacaaag agcgaacgga actgctcgac
      961 catcccaaaa tcctggaata cttccaaaaa gaagtagatc gcttaacccc ggatctggcg
     1021 agttacgaaa aaccgaaaaa agttgtgctt ctggaacagg aattgaccat tgaagggggt
     1081 gaactgaccc cgaccctcaa agtcaaacgc cgcgtggtgg atctcaaata caaagacgtg
     1141 attgacaaga tttaccagga aaaataatgc ccgttccggc taaggactga gaattcaatc
     1201 cacctgtccg attgaccaga gagtttgttc ttagaaattg ctatgaaatt gaaagttgtc
     1261 atccatcaag ctgaagaagg cggatactgg gcagaagtgc cttcaatccc aggatgtgcc
     1321 acccaaggtg aaacgttcga agaattacta aacaacctgt atgaagcagt tgaaggttgt
     1381 ttagcggtgg atgtgtcgcc atttctgacg gatgaacgtg ccagagtcat ggaaattgca
     1441 gtatgaaatc catctcagga aaagaatttg ccaaaattct tgaacgccac ggatggaatc
     1501 tactcagagt tcagggaagt catcacattt atggaaaggc tggactccca gtcagaattt
     1561 cagttccaat tcatgggaac cagcctttga aaacagggct tctccgaacc ttattaaaag
     1621 ccgctggatt gtctgaatcc gacctgtaat tttatttttt tgcaaaggaa gttgttcatg
     1681 aaccgcaagt tggaaaaagc agcagtacta ggagccggag ttatgggcgc acaaattgcc
     1741 gctcatctgg cgaatgcagg cgtgagtgtc accctgctcg atattgtgcc ccccagctta
     1801 agcccggctg aagaagccgc cgggctcaca ctggccgatc cgaaagtccg caatcggttt
     1861 gccgctcagg ccgtggaaaa catgcacaag atgaagctgg caccgttttt cactcgcgaa
     1921 caggcagcgt tgatccgcac ggggaacttt gaagaccatc tcgaatggct aaaagacgcg
     1981 gactggatca tcgaagcgat tgtcgaacga ctcgacctca aaaaagggct ctatgagcgc
     2041 attgaaccct tccgcaaacc aggcagcctg attaccacca atacttcggg cattccgatt
     2101 ggttcgcttt cggttggacg atctgatgat ttccgcacca attttttagg aacgcacttt
     2161 tttaatccgc cacgctatat gcatctggtg gaaatcatca ccacgccgga taccaaccct
     2221 gaagccgcct gtttttcggc tgggctctgt gacaaagtcc tgggcaaagg actggtctat
     2281 gccaacgaca caccaaactt tattgccaac cggatcggga tcttcggcat tttgcagacg
     2341 atcaaggtca tgaccgaagg tggttattcg atcaccgaag ttgacaaaat gagcggcccg
     2401 gcgattggcc gtcccaagag tgcaaccttc cgcactgccg acctggccgg cctggacacc
     2461 accatgctgg tggcgggcaa cgtttatgac aatgctccgc acgatgaata tcggacgacc
     2521 tttattgtcc ctgatttctt caaagatatg gtggcccgca agtggctggg cgataaaacc
     2581 ggtcagggct tttataagaa agtccagggc gctggtggaa gagaaatcct ggcgcttgat
     2641 tacaagacga tggaatatgt cccacagccc aaagtgagct tcccatcgct tgaaaatgcc
     2701 aaaaatatca gtgatttggg cgaacgactt cgcacactgg tctatggcaa ggaccgggtc
     2761 ggtgaattcc tctggccagt catcagcgaa acgttgctct ataccgcccg ccggattcct
     2821 gaaattgcca acgatccgat ccaggtggat aaagccatgc gctggggctt taactgggaa
     2881 atgggtccgt ttgaaatgtg ggatgccctg ggcgttgaaa aaaccgtcaa gaagctccag
     2941 gccgaaggcc gcgagattcc gcaactggtc caggatctgc tcaaatcagg ccgcaagagc
     3001 tggtatgaat cagaaaaagg catcacccgc gtgtttatgc ccgccgtcaa agaacacgtc
     3061 gaagttcctg accagtcagg ggtcatcgtg ctcaaggcgc tcaaggaccg tgaaaaagtt
     3121 atcaagaaaa attctggtgc aacgctggtt gacctcggtg atggtgtggc ctgtcttgaa
     3181 ttccacacca agatgaacac catcggcagc gacacggttt cgatgatgca gcaggcggtc
     3241 aaagaagttg aaaagaactt tgaaggactc gtcatcagca accaggccga taatttctgt
     3301 gctggtgcca acctgatgat ggtgatgctg gccgtgcagg aaggtgaatg ggatgaactc
     3361 aaccagatga ttcgtgcctt ccaaaacgcc aacatgaatc tgcgctattc agccaaacca
     3421 gtcgtcgtgg ccccgtttgg cctgaccctt ggcggcgggt gcgaaatcac cctccatgcc
     3481 gacaa
//
LOCUS       DAOQDO010000383         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20158_length_3483_cov_2.897900, whole genome shotgun sequence.
ACCESSION   DAOQDO010000383 DAOQDO010000000
VERSION     DAOQDO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20158_length_3483_cov_2.897900"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKZ53_07470"
     CDS             complement(<1..485)
                     /locus_tag="PKZ53_07470"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNJ40311.1"
                     /translation="MSSFTAEDRAAMADSVRRLLADLCTEAGIRRAMESEAGYDPALW
                     TALVDLGVPGLVIDPAHDGLGGGPVELEAIMEEAGAALLPAPLFAVTMAAALIAKVSE
                     IYCIGGVQAVAALAYGTETIAPVDVIAGPGNAYVAEAKRQAYGRVGIDMVAGPSEVCI
                     VA"
     gene            complement(579..1937)
                     /locus_tag="PKZ53_07475"
     CDS             complement(579..1937)
                     /locus_tag="PKZ53_07475"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNJ40312.1"
                     /translation="MFTDTLEGYPLSLQQKRLWHFQTDQTPFRTTAAILINANLNVDK
                     LKQALEQIVGRHEILHTVFRQLPGMKLPVQVVLDQPAIEFRFLDADPGMFQNQGELIE
                     AIFQTELLRKADLEQGPVLQVAFLALQPGKSMLFVTIPALCADAQTMGNFINELEILS
                     TTDIAGSDLDPEPVQFVQFSEWQHSLLESDEAADGKAFWNRIDRTSITTRLPFGLPAA
                     LPFEVESLKIPMSSRLRRELRSLAEDHAVAFQEFYLSSRANPSEDRRIEPAPALDTLE
                     ESTPEEQIEAASQALSTALGVELLQRIRGNSPSFFEQVIVNLLVGMGYGGSHRNAAQQ
                     LGKSGDGGVDGIINEDVLGLDRVYVQAKRYAEGSTVGRPEVQAFVGSLVGLGATKGVF
                     VTTGSFSAHAVEYVRHLSQRVILIDGLRLTELMMQHGVGVRVTRVIEFKRVDEDFFGE
                     DE"
     gene            2735..>3483
                     /locus_tag="PKZ53_07480"
     CDS             2735..>3483
                     /locus_tag="PKZ53_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40313.1"
                     /translation="MHLRKKLSSSWKGLAGLALVLAVFGLLDLPAMRIHSSFADNGKL
                     PIKNATGAKQEATTQQPSSLRKEVEPAPELDDFQQKLLNQAAEDAAHREAEAKGIRVN
                     PLIPAPTNDLCAAAELIPGAGPFPYVTSIVNITTAGDEAIPPSPSCATLPVSRSIWFS
                     FTPTTTGSYRFSTSASATSTTSFAPAPTLSSVEDTDVAIYSSNTNMCGGTLTQVGCSD
                     DEGVSFQSEATATLTAGQTYFVVAWKFGASAP"
BASE COUNT          803 a          982 c          899 g          799 t
ORIGIN      
        1 gccacaatgc acacttcgga aggacccgcc accatatcga tgcccacacg gccataggcc
       61 tgacgcttgg cttccgctac ataggcatta cccgggcctg caatcacatc caccggggcg
      121 atcgtttctg taccatatgc cagtgctgcc acggcctgca cgccgccgat gcagtaaatc
      181 tcgctcactt tagcgatgag cgcggcggcc atcgtcaccg cgaagagcgg ggcgggcagc
      241 agggccgcgc cggcttcctc catgatcgct tcgagctcga ccgggccgcc gccgaggccg
      301 tcatgggcgg ggtcgatgac gaggccggga acgccgagat ccaccagcgc cgtccacagc
      361 gccggatcgt agcccgcctc gctttccatc gcgcggcgga tgccggcttc ggtgcagaga
      421 tcggccagca ggcggcggac ggagtccgcc atcgcggccc ggtcctcggc cgtgaaactg
      481 ctcatggacg cttctcccgg ttcttgtcgt tcagccatcg tcgcgcgccc cggcaagggc
      541 ggcaagtggc cgtaacggca aggttggggc gaccttggct actcgtcctc gccgaagaag
      601 tcctcgtcga cccgcttgaa ctcgatgacc ctagtcactc tgacgccgac tccgtgctgc
      661 atcatcagct cagtcaggcg caaaccatcg attaggatta cgcgctgaga caagtggcgc
      721 acatactcga cggcgtgcgc gctaaacgaa ccagtcgtta cgaacacgcc cttcgtagca
      781 cccaagccaa ccaagctgcc aacaaaagcc tgaacttctg gacgtcctac ggttgagcct
      841 tcagcgtatc tcttcgcctg tacgtagacc cgatcaagtc ccagcacatc ctcgttgatg
      901 atgccatcaa caccgccgtc gccggacttt ccaagctgct gggcggcgtt ccggtgcgaa
      961 cctccataac ccatgccaac caacaggttc acgatgacct gttcgaaaaa cgatgggctg
     1021 ttgccccgaa tacgctgcaa caactcaacg ccgagcgcgg ttgatagagc ttggctagca
     1081 gcctcgattt gctcctcggg tgtggactcc tccagggtat cgagagctgg cgcgggttca
     1141 atccttcgat cctcgctcgg gttggctcgc gacgacaggt agaattcctg aaatgccacc
     1201 gcgtggtctt cggccagaga ccggagttcc cgccgcaacc ggctgctcat cgggattttg
     1261 agtgattcga cttcaaaagg cagtgcggct ggaagtccaa agggcaaccg ggtggtaatg
     1321 gaagttcgat caatcctgtt ccaaaaggct ttgccgtcag cggcttcgtc ggattccaac
     1381 aggctatgtt gccattcaga gaactgcaca aactggactg gctccgggtc caggtctgac
     1441 ccggcgatgt cagttgtgga aaggatctcc agttcgttga tgaagttccc catcgtctgc
     1501 gcatcggcac agagcgccgg aatggtgacg aaaagcatag attttcccgg ttggagcgcc
     1561 agaaatgcca cctgcaggac cggcccctgt tcaagatcag ccttgcgcag caattcagtt
     1621 tggaaaatcg cttcaatcag ttcgccctgg ttttgaaaca taccggggtc ggcatccaga
     1681 aagcgaaact cgatggctgg ttgatcgagt accacctgaa ccggcagttt cattccgggc
     1741 aactggcgga acaccgtatg caggatttca tggcgcccga caatctgttc aagtgcctgt
     1801 ttcaatttgt caacattgag gttggcattg atcagaatgg ctgccgtcgt gcgaaacgga
     1861 gtctggtcag tttggaaatg ccacaggcgt ttttgttgga gcgaaagtgg atagccttcg
     1921 agtgtgtcag taaacatggc agattggtgt atcagatgaa ttgggatgag agctggtgga
     1981 agtcgggaac tataccagaa aaggcaactc cctggcgcgg ggcactgaag accgcaagca
     2041 cttcagaaat cgaaaagttg tcggaaattt cggaaataaa tgtactgttt ttataacatc
     2101 aattacacgc ttagatgcct gcattccaga tcaagaaacc attcaaatac aagaggttga
     2161 taggatctgt tgaaattggg caatgtggct cgaaaatgaa tcaaattggt gctttttttc
     2221 ttgttctgcc aaagcaaatt cagttatggt ttgaaatgca ttcacaataa attccagcac
     2281 tcctgtcgtg gatagaacat tccccttcct ggttcgcaat ctgttgtctt tgcccgtttt
     2341 tttcaccagt cagtctcctt ttcataagga agtctgagtc gttctttggc tattgtatat
     2401 agcgttaggg aatagcatat tcctgtgcat cctcttgaat tgaaaagttt tgcaggtttc
     2461 accaggtcta ttgagttccc ttttccatca aagctgtcac tggtttttcg ctgtatctga
     2521 actaccctta aacacttttt ccggtgtgat tgagtgcttt taccaaggtg ctttggttcg
     2581 ggatgcgctc ttttcacaca ctgatcaact tagaaatgaa ccgattgaga aaaaagtaat
     2641 tcactccacg tctcaaaact caattttgat cccgaatgcc ttgagcacgc ttaaaaaatt
     2701 tcaacctcca ctccaaactc ttgggagacg aactatgcac ctccgaaaaa aactatccag
     2761 ctcctggaaa ggacttgctg gtctcgcact ggtccttgcg gtctttggcc tgctggattt
     2821 gccagccatg cggattcatt catcgtttgc tgacaacggc aaactcccga tcaaaaacgc
     2881 cactggagcc aaacaggaag cgacgacaca gcaaccatcg tcactccgaa aagaagtaga
     2941 gccagcgccg gaactcgacg actttcagca gaagctctta aaccaggcgg ctgaagacgc
     3001 ggcccatcgg gaagctgagg caaaagggat cagggtcaat ccactcatcc cggccccaac
     3061 caatgatctg tgcgccgccg ccgaactgat tcccggcgcc ggtccatttc cctacgtgac
     3121 cagtattgtc aacattacca cggcagggga tgaagccatt ccgccatcac cgtcgtgtgc
     3181 aactcttcct gtctcacgca gtatctggtt ctcatttaca cccacgacga ctggcagcta
     3241 tcgcttttca acctcagcgt cagcaacgag tacaaccagt tttgcccccg caccaacgct
     3301 ttcatccgtt gaagatacgg atgtcgcaat ttacagcagc aacaccaata tgtgcggtgg
     3361 aactctgacc caggtgggtt gtagcgatga tgaaggcgtt tcatttcagt cggaagccac
     3421 tgccacgctg actgccggcc agacctattt tgtagtggcg tggaaatttg gtgcatcagc
     3481 ccc
//
LOCUS       DAOQDO010000384         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20165_length_3482_cov_3.690983, whole genome shotgun sequence.
ACCESSION   DAOQDO010000384 DAOQDO010000000
VERSION     DAOQDO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20165_length_3482_cov_3.690983"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..3161)
                     /locus_tag="PKZ53_07485"
     CDS             complement(75..3161)
                     /locus_tag="PKZ53_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ40314.1"
                     /translation="MINTILRFSVTHRLLILLLAMMLIGGGWYSFRRLPIDAVPDVTN
                     IQVQILTAAPALSPLEIERQITFPVEIAMSGLPDIEEIRSVSKFGLSAVTVVFHDHVN
                     IYFARQLVSERLSQAREQIPANLGSPELGPITTGLGEVYQYELKADHGSGYDPTSLRT
                     VQDWIVRRQLLGVPGITEVNSFGGLEKQYQVQVDPTRLQSLGLTLRDVLDAVARNNAN
                     VGGAYIEHGAEQYVVRGIGLVANPAEIAEIVIKTGPEGIPVFVRDVATVVTGAVVRQG
                     AVTADGEGEIVCGITLMLKGANSRTVVEDVKSRVEQVKKSLPKGVSLIPFYDRTELVN
                     RTLRTVITNLVEGALIVILVLIVLLGNWRGALLVAAVIPLSMLFAAIGMNVFGVSGNL
                     MSLGAIDFGLVVDGAVIMVENAIRRIGERQHQLGRTLSKEELKETICSAAAEVRKATM
                     YGELIIAVVYLPILSLTGIEGKMFRPMAMTVIFALAGAMILSLTFVPAAMTLVFRGPI
                     SERESGLIALVKRLYRPTLAWVLQFRSQTLAVATGLVVIAAAIFPLLGAEFIPRLDEG
                     SLAIQVQQLPSVSLTQSIKTTTEVERVLKTFPEVTRVVSKTGRAEVATDPMSIDFSDL
                     YVGLKPPSEWTSAKTRETLVSKMAAALEANVPNAQFSFSQPIELRVSELISGVRSDVA
                     IKLFGDDLETLKVKADEMARAVSRIPGAADVKVEATAGLPQLQIVPNRREIARHGINV
                     EDVNTLVESIVAGKEAGVVYEGEQRFGLVVRLDAAVGRDIDSIKNLQVTAPNGARIRL
                     AELATVTVVEGPAQISREDTHRRISVELNIRGRDIASFVAQAQAELNRTVQLPAGYYV
                     TWGGQFENLQQASERLLIVVPIAMFLIFILLFASFGSVWQAVLIYTGIPFAAVGGVLA
                     LWLRGMPFSISAGVGFIALFGVAVLNGLVLVSAISQLQKSGRTTIQAIWEGAQSRLRP
                     VVMTALVASLGFLPMALATSAGAEVQRPLATVVIGGLVTSTLLTLVVLPMLYGWKE"
BASE COUNT          938 a          950 c          845 g          749 t
ORIGIN      
        1 atttggaagg aaaacgttag ggttggcaaa agcctaacag gacaaaaagg acataatttc
       61 gaaacccctg aaccctattc tttccatcca tagagcatcg gtaaaaccac cagagtcaat
      121 aacgtagagg tgaccagtcc cccaatcacc acggtggcga gcggacgctg gacttccgct
      181 ccggcagaag tggccagagc cattggtaaa aatccaagac tggcgaccag tgcggtcatc
      241 accacgggtc gcaggcgtga ctgggcaccc tcccaaattg cctgaatcgt ggtgcgaccc
      301 gatttctgaa gctgactaat ggcactgacc aggaccaggc cattcaaaac tgcgactccg
      361 aagagcgcaa taaacccaac gccggctgag attgaaaatg gcatgccacg caaccagagc
      421 gccagcacac cacccacggc ggcaaaagga atccccgtgt aaatcaacac cgcctgccaa
      481 accgagccaa acgacgcaaa caacaagata aaaatcaaaa acatggcgat tggcaccacg
      541 atcaacaatc gctctgacgc ctgctgcaaa ttttcaaatt gcccgcccca ggttacatag
      601 tacccggctg gaagctgaac cgttcggttg agttcagcct gagcctgagc gacaaaactg
      661 gcgatgtcgc gtccacggat gttgagttcg acgctgatgc ggcgatgggt gtcttcgcgt
      721 gaaatttggg cggggccttc aaccacggtc accgttgcca gttcggccag tcgaatacga
      781 gcaccatttg gggctgtcac ctgcagattt ttaattgaat caatgtcgcg cccgacggca
      841 gcatccagcc tgaccacaag cccaaaccgc tgttcgcctt catagacaac cccggcttct
      901 ttgccggcca cgatggattc aaccagtgtg ttgacatctt cgacattgat cccatggcgg
      961 gcaatttccc gacgattggg cacaatttgc aattgtggaa gtccagccgt cgcttcgact
     1021 ttgacatcag cggcaccagg aatgcgactg acagcacggg ccatttcgtc ggctttgacc
     1081 ttgagtgttt ccagatcatc gccaaacaac ttgatcgcga catcagaccg aacaccggaa
     1141 atcaattcag agacccgtaa ttcaatcggc tgcgaaaaac tgaactgtgc atttggaacg
     1201 ttggcttcga gggctgccgc catttttgaa accagcgttt cccgagtttt ggcgcttgtc
     1261 cactcagacg gtggtttgag cccaacatac agatcgctga aatcaatact catcgggtca
     1321 gtggcaactt cggcccgacc cgtttttgaa accacgcggg tgacttccgg aaacgttttg
     1381 agtacacgtt cgacctcagt tgtggttttg atggattggg tcaatgacac acttggaagc
     1441 tgctgaacct gaatggccag cgagccttca tccaggcggg gaataaactc ggcgccaagc
     1501 aatggaaaaa tcgcagccgc aatcacgacc agacccgttg ccaccgcaag cgtttgcgac
     1561 cgaaactgca atacccaggc cagtgttggg cgatacagcc gtttgaccag agcaatcaac
     1621 ccactttcac gttctgaaat cggacctcga aataccagcg tcatggcggc tggaacaaag
     1681 gtcaaggaca ggatcatcgc cccagccaga gcaaaaataa cggtcatggc cattggccgg
     1741 aacattttcc cttcgatccc ggtcaggctg agaatcggca aatacaccac ggcaataatc
     1801 aactcgccat acatcgtggc tttgcggacc tctgcggcgg ctgagcaaat tgtctctttg
     1861 agttcctctt ttgaaagtgt tcgaccaagc tggtgttgtc gctcgccaat ccgtcgaatg
     1921 gcgttttcca ccataatcac cgccccgtcc acgaccagcc caaaatcaat cgcccccaag
     1981 ctcatcaaat ttccggaaac gccaaagaca ttcatcccaa tcgcggcaaa gagcatcgaa
     2041 agcggaatta cggctgccac cagtaacgcc ccgcgccagt ttccgagcag cacaatcaaa
     2101 accagaatca caatcagcgc accttcgacc aggttggtga tgactgttcg aagggtccgg
     2161 ttcaccagtt ccgtccggtc ataaaaggga atgagtgaga cgcctttcgg aagtgatttt
     2221 ttgacctgtt cgacgcgaga tttgacgtct tcgaccacgg ttcgtgaatt ggcgcctttg
     2281 agcatcaagg taatgccaca cacgatttcg ccttcaccat cagccgttac ggctccctgg
     2341 cgaaccaccg cacccgtgac caccgtggct acatcccgaa caaaaaccgg aatgccttcc
     2401 ggaccggtct taatcacaat ctcggcaatc tcggcaggat tggccactaa tccaattccg
     2461 cgcacgacat attgctcggc accgtgttcg atataggcgc cgccgacgtt ggcattgtta
     2521 cgagccaccg catcgagcac atcgcgcagt gttaacccca ggctctgcag ccgggtcgga
     2581 tctacctgta cctggtattg cttttccaat ccgccgaaac tgttgacttc agtaatacca
     2641 ggaacgccaa gcaactgccg ccgaacaatc caatcctgaa ctgttcggag cgacgtcgga
     2701 tcatagccac tgccatggtc ggctttgagt tcatattgat aaacttcgcc aagcccggtg
     2761 gtaatgggac cgagttctgg cgaaccaaga ttggctggaa tttgttcgcg agcctgtgac
     2821 aaccgttctg aaaccagttg ccgggcaaag taaatattga cgtggtcgtg aaagacgacc
     2881 gtcaccgctg ataacccaaa cttagaaacc gagcggattt cttcgatgtc tggcaggccc
     2941 gacatcgcaa tttcaaccgg gaatgtaatt tgtcgttcga tttcgagggg cgatagggcc
     3001 ggagccgccg tcagaatttg aacctgaata ttggttacat ccggcacagc atcaatcggc
     3061 agtcgccgaa aactgtacca tccgccacct atcagcatca tcgccagcaa caaaatcagc
     3121 aaccggtgcg tgaccgaaaa tcgaagaatg gtgttgatca taaatttagg gttccgggtt
     3181 ccaggaatga agaattgaga atgaagaatt gagaatgaag aattgagaat gaagaattga
     3241 gaataaagaa ttgagaatga agaattgaga atgaagaaat gtatgaatta ttttaaggct
     3301 atccagttga ataattggta gttacctgtt ttgttgtgtg gtgttcattc gggtttcgta
     3361 cattcgacct tctgaggtac aggtaagtac cttaccgaaa atttccaaac attttaacca
     3421 cggaaaccac ggaaaagaat caagcactta ccaaacccaa tatctcagaa aatttatgac
     3481 aa
//
LOCUS       DAOQDO010000385         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20169_length_3482_cov_3.084039, whole genome shotgun sequence.
ACCESSION   DAOQDO010000385 DAOQDO010000000
VERSION     DAOQDO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20169_length_3482_cov_3.084039"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKZ53_07490"
     CDS             complement(<1..1097)
                     /locus_tag="PKZ53_07490"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNJ40315.1"
                     /translation="MDNVLAVILGGGQGTRLFPLTHERSKPAVPLGGKYRLIDIPVSN
                     CINSGIIKIYILTQFNSASLNRHVARTFRFSQFSSGFVDILAAEQTPDNPHWFQGTAD
                     AVRQNFRHIKDVGADTIIILSGDHLYKMDYSKFINAHWDAKADVTVSVTAVPPEEASE
                     FGLLKVDDTGRIVEFKEKPRGDALESMRVNTELMGLSPEEAELRPYLASMGIYVFNLQ
                     VLEDLLNQQGRIDFGREVIPSAINNYKVNSYLFKGYWEDIGTIGAFFRANIELTDVLP
                     RFNFFDTRKQIYTRPRFLPGTKIRNAQTHDSIICEGCIINEAIVRRSVVGIRSRIESG
                     SRLDHVLMMGADDYESIDEMRRNKETNQPNIG"
     gene            1243..3411
                     /gene="recG"
                     /locus_tag="PKZ53_07495"
     CDS             1243..3411
                     /gene="recG"
                     /locus_tag="PKZ53_07495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNJ40316.1"
                     /translation="MLTLATPLAELHLYLPRVGKKTATKLATEVAELLEMTDLADPTV
                     EDLVYYLPFRYEDRAQLMSSKDLADGMKAALEVELTTTEYAPIRTKTGRALSIFQITG
                     LDSAGRLRAMWWNQPYLTNVFEKGQRVVFYGEWKYSNRHNCFQIENPEYEILNETSET
                     ASEFDDAGTIHTGRRVPIYRKLGSFRTRALRTIMFRMLEQLTEAGEEWLPESLMQRYG
                     FPSRLDAFRQVHFPAMDHWLDDYQCGRSKAHARLATEEFLLLAVALNRRRLALRETTK
                     GTAMTVNDHIREAVRRVLPFKPTNAQKRVIREIVGDMTSPHPMNRLLQGDVGAGKTMV
                     ALQAMIVAIENGYQVAMMAPTEILVEQHALNLKRYLKDTSYRVESLTGRLKPKEKLAL
                     RETIASGEVDLVIGTQALIQESTRFARLGLVVIDEQHRFGVIQRTALSQRGLQPDVLV
                     MTATPIPRSLAMTAYGDLDISVLDELPPGRQPIRTAIRTNAEREKVYDFLQREATEGR
                     QVYIVFPLVEESEKMDLKAATQAAEELQTEIFPSLKIGLLHGKMKSVEKEAVMATFSA
                     GETQILVSTTVIEVGIDVPNSSVMVIEHPERFGLAQLHQLRGRIGRGAAKSYCILMLA
                     PKTSQEARDRLELFAASSDGFVIAEKDLELRGPGEVFGTRQSGVPLFRVGNIVRDRDW
                     LETAQQTARDLMREHPHSREVLRLVELSQIRYPQAEQQLH"
BASE COUNT          860 a          849 c          971 g          802 t
ORIGIN      
        1 ccaatgtttg gctggttggt ttctttgttg cggcgcattt catcaattga ttcataatca
       61 tcggcaccca tcatcaacac atggtcaagc cgggaaccgc tttcaatccg gctccggatt
      121 ccaaccacag agcgtcggac gatggcttcg ttgatgatac agccttcgca gataatcgaa
      181 tcgtgggtct gagcgttacg gattttggtg ccgggcagaa aacgtggccg ggtatagatt
      241 tgcttgcggg tgtcaaagaa attgaagcgt ggcagcacat cggtgagttc gatattggcc
      301 cggaaaaagg cgccaatcgt tccgatgtct tcccaatatc ctttgaacag ataactgttc
      361 actttatagt tgttgatagc tgatggaatc acttcgcggc caaagtcaat tcgaccctgc
      421 tggttgagca agtcttcgag cacctgcagg ttgaatacat aaatccccat ggaagccaga
      481 taggggcgta attcggcttc ctcaggagaa agtcccatca gttcggtatt gacgcgcatg
      541 ctttcaagtg catcacccct gggtttttcc ttgaactcaa caattcgtcc agtgtcgtca
      601 accttgagca gtccaaattc tgaggcttct tcaggtggca cggcggtcac ggaaaccgtc
      661 acatctgctt tggcatccca atgggcattg atgaacttgg aataatccat tttgtacagg
      721 tggtcacccg acaaaatgat gatggtatca gcgccgacat ctttgatatg acggaagttc
      781 tgccgaacgg catccgccgt accttgaaac cagtgtgggt tgtcaggggt ttgttcggcg
      841 gccagaatat ccacaaaccc cgaggaaaac tgactgaagc gaaaggttcg ggcaacgtgg
      901 cgattgagtg aagccgagtt aaattgcgtc aggatgtaga ttttgatgat tccagaattg
      961 atgcaattac ttaccgggat atcaattaag cgatacttcc cgccaagtgg aacagcgggt
     1021 tttgatcgtt cgtgggtgag aggaaagagc cgcgttcctt gaccgcctcc caaaatcaca
     1081 gccagtacat tgtccataca aaagagatgc ctccagaaaa aagatgcgtg gattcgtgaa
     1141 cggagcttgg gtattgttgg aaaaactgag acgctcacct gatcggatga ttgggctgca
     1201 tcctttacca ttcgccttta cctgtcaagg aaaagaacag aaatgctgac acttgccaca
     1261 ccacttgcgg agttgcacct gtacctgccg cgtgtcggaa aaaaaacagc aaccaaactg
     1321 gctacggaag tggctgaact cctggaaatg acggatttgg ctgacccaac ggttgaagat
     1381 ttggtgtact acctgccatt tcgctacgaa gaccgcgccc agttgatgag cagcaaagac
     1441 ctggctgatg gcatgaaagc tgccctcgaa gtcgaactga cgaccacaga atatgcccca
     1501 attcgaacca aaaccgggcg ggcactttcg atttttcaaa tcacgggtct ggattcggct
     1561 ggtcggttac gcgccatgtg gtggaatcag ccatatttga ccaacgtttt tgaaaagggg
     1621 cagcgggtgg ttttctacgg cgaatggaag tactcaaacc ggcacaattg ctttcaaatc
     1681 gaaaatcccg aatatgaaat tctgaatgaa acatcggaaa cggcgagcga atttgatgat
     1741 gccgggacga tccacaccgg acggcgggtg ccgatttacc gcaagctggg atcgtttcgg
     1801 acacgggccc tgcgcacgat catgtttcgc atgctggaac agctcaccga agcgggtgaa
     1861 gagtggctgc cggaatcatt gatgcagcgg tatggatttc catcccggct ggatgccttt
     1921 cgacaggttc actttccagc gatggatcac tggcttgacg actatcagtg tggccgttca
     1981 aaagcgcatg cccgactggc gactgaagaa tttctgctcc tggccgtggc gctcaaccgc
     2041 cgtcgactgg ctctgcgcga gacgacaaaa ggcacggcaa tgacggtcaa tgaccatatc
     2101 cgcgaggctg ttcggcgagt attgccgttt aaaccgacca atgcccaaaa acgggtgatt
     2161 cgggaaattg tgggtgatat gacctcaccg cacccgatga atcggttact gcaaggtgat
     2221 gttggggctg gaaaaacgat ggtggcgctc caggccatga ttgtggcgat tgaaaacggc
     2281 tatcaggtgg cgatgatggc gccgacggaa atcctggtcg agcagcatgc gttgaatctg
     2341 aaacgttatc ttaaagacac cagctatcgg gtcgaaagcc tgacgggccg gttaaaacca
     2401 aaggaaaaac tggcgcttcg cgagacgatt gccagtgggg aagttgatct ggtgattggg
     2461 actcaggcgc tcatccagga aagtacccgg tttgcccggc tgggactggt cgtcattgat
     2521 gaacagcacc ggtttggcgt aattcaacgg accgcgcttt cacaacgcgg tctccagccg
     2581 gatgtactgg tgatgacggc aacgccgatt ccgcgttcgc tggccatgac ggcttatggc
     2641 gacctggaca tttccgtact cgatgaactt ccacctggaa gacagccgat ccggacggcc
     2701 atccgtacca atgccgaacg tgaaaaggtc tacgattttc tgcagcggga agcgactgaa
     2761 gggcgtcagg tctatattgt gtttccactg gttgaagaat ctgaaaagat ggatctcaag
     2821 gcggccaccc aggcagcgga agaattacaa accgagattt ttccatcgct caaaattggg
     2881 ttgctccacg gaaaaatgaa gtcagtggaa aaagaagctg tgatggccac cttttccgcc
     2941 ggggaaaccc agattctggt ctcgaccacc gtgattgaag tcggtattga cgttccgaat
     3001 tcgtcagtga tggtgattga gcatcccgaa cggtttgggc tggcccagtt gcaccagctt
     3061 cgagggcgaa ttggacgtgg tgccgccaaa tcctattgta ttttaatgct ggcgccgaaa
     3121 acctcccagg aggcccgaga ccggctggaa ttgtttgcgg cttcaagcga cgggtttgtg
     3181 attgctgaaa aagacctgga attgcgcggc cccggcgaag ttttcggcac ccggcagtct
     3241 ggagttccgc tttttcgggt tggaaacatt gtccgtgacc gcgactggct cgaaaccgct
     3301 cagcaaactg cccgcgattt aatgcgcgag catccccatt cccgcgaagt gctgcgactg
     3361 gtcgagcttt cccagattag atatccccag gcggagcagc aattgcatta gggctgagaa
     3421 aaccagggct gaagacattg ggttgaagac attgggctga agacattggg ctgaagacat
     3481 cg
//
LOCUS       DAOQDO010000386         3480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20187_length_3480_cov_3.217810, whole genome shotgun sequence.
ACCESSION   DAOQDO010000386 DAOQDO010000000
VERSION     DAOQDO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20187_length_3480_cov_3.217810"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PKZ53_07500"
     CDS             <1..1373
                     /locus_tag="PKZ53_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ40317.1"
                     /translation="ILHSSFFILNSSFFILHSIKELALKLSSSELTRLAETLNTSQLR
                     QILSGPAETKSASRKKKALVEDLSPAPPRTLSKLNRMELIVELSIQAQDQPALAHRLI
                     EAVELTPRQLRDWLEEHEPKAPKAGLDKIVAILTRSYQKGHHAAGVPASAVVQLLASG
                     GVTHVLKELIEFEKSAGAKAEESLDLHALMLRRRRREELDQLLAGIGAPEVQPFQPDD
                     FQLEAVDLVLNQNADVMVSAPTGSGKTWIAEQAIRGILERGQSCWYTSPLKALSNDKF
                     REFSRIFGPENVGIITGDRRVNPDAPLKIGTTEIYRNGLYDAMNGLGFSDRTLSQVRL
                     VVFDEVHYLGDRERGVAWEESIIYTPPDARILMLSATVGNSQELADWVTWTRGVPCHL
                     VYHPDRPVPLRTAFVHTLGRLQPLLSDQDGGTHLHADIIALYSNAFVELEARKNRRRP
                     FYRRFK"
     gene            1452..2285
                     /locus_tag="PKZ53_07505"
     CDS             1452..2285
                     /locus_tag="PKZ53_07505"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNJ40318.1"
                     /translation="MSKDQSKRPLPLKAYKNLDFLNSNSARVLRIMSEYLEPESRFRH
                     YKVKDTIVFFGSARIRPREEVEKELNNDGLEDGQRDKLDRALEMSRYYDDAVELARLL
                     TEWSKSLKERQRRFLICSGGGPGIMEAANRGAALAHGKSIGLNISLPFEQTPNPYISN
                     ELSFEFHYFFMRKFWFIYLSKALVIFPGGFGTLDELLEVLTLIQTKKLRRPIPIVIYG
                     EQYWREVLNLDALEKWGMINKEDLKLFRFADTPQAAFEYLRDQLQMFYGEEAEASPFN
                     E"
     gene            2448..3374
                     /gene="rnz"
                     /locus_tag="PKZ53_07510"
     CDS             2448..3374
                     /gene="rnz"
                     /locus_tag="PKZ53_07510"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007306992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNJ40319.1"
                     /translation="MQITFLGTSSGVPTRARNVSSVILSLTQRGEQWMFDCGEGTQHQ
                     FFRSNLSISQLRRIFVTHMHGDHTFGLMGLLSSAGLTGRMTKVDLYGPAELKDLIHAC
                     QGFTQTQFPYNLNIHPVKPGLVFEDHEYTVSCATLRHRIPAYGYRVQEKDRPGQFLVE
                     KAKALGIPFGPLYGELKRGNTITLADGRIINGAELCGPPKPGRSVVYCTDTIYSESSV
                     RLAKDADVLIHESTYAHEDEPLAHRALHSTTRMAARVALEAGVKQLLLTHFSPRYTNE
                     SAITLDDLLKDARSIFPNTELAHDFLVWKVDK"
BASE COUNT          849 a          910 c          872 g          849 t
ORIGIN      
        1 acattcttca ttcttcattc ttcattctca attcttcatt cttcattctt cattcaatta
       61 aggagcttgc tttgaaactg tcatcatctg aactgactcg gctggcggaa acgctcaata
      121 catcgcaact gcgccagatt ttatctggtc cggcagaaac caaatctgcc agccgaaaga
      181 aaaaagcatt ggttgaagat ctttccccgg cacctcccag gacgctttca aagctcaacc
      241 ggatggaatt gattgttgaa ctttcaattc aagcccagga ccagccagcg cttgcccacc
      301 gtttgattga agccgttgag ttgactcccc gacaattgcg tgactggctt gaagagcacg
      361 aaccaaaagc acccaaagcc ggcctggata aaattgtggc cattctgacc cggtcatatc
      421 aaaaaggcca tcatgcggcg ggtgtaccag cttcggcggt tgtccagttg ctggcttcag
      481 gtggagttac ccacgtgttg aaagaattga ttgagtttga aaaatcagcc ggcgccaaag
      541 ccgaggaatc gcttgattta cacgcactga tgctccgccg acggcgccgg gaagaacttg
      601 atcagttgct ggctggcatt ggagcgccgg aagttcagcc atttcagcct gacgattttc
      661 agcttgaagc agttgatctg gttctaaacc aaaacgcgga tgtgatggtt tctgcaccaa
      721 ccggttcagg gaagacctgg attgccgagc aggccatccg tgggatattg gagcgcggtc
      781 aatcctgctg gtatacctct ccgctgaaag ccctttccaa tgacaaattt cgggaattca
      841 gtcggatttt cggcccggaa aacgtcggta tcatcaccgg tgatcggcgg gtcaacccgg
      901 atgctccgct caaaattggc acaaccgaga tttaccgcaa cggcctctat gacgccatga
      961 atggccttgg attttcagac cgaacactca gtcaggttcg gctggtcgtc tttgatgaag
     1021 ttcattattt gggtgatcgc gaacgtggcg tagcctggga agaatcaatt atttataccc
     1081 cgcctgatgc ccgcattctg atgctttcgg ctacggttgg aaactcgcag gaactggcag
     1141 attgggtgac ctggacgcgt ggtgtgccct gtcatctggt gtaccatcca gatcgcccgg
     1201 tgccgctccg aaccgccttt gtccacaccc tgggacggct ccagccactc ttatccgatc
     1261 aggacggggg aacacatttg cacgctgata tcattgctct gtacagtaat gcctttgtcg
     1321 aactcgaagc ccgaaagaac cgccgccgcc cgttctatcg ccgatttaag taatgagtga
     1381 agtcataaaa tagcaagtag tcaccaagcc cccagtcatc ttgccattct gtcataagga
     1441 gttgcccctg aatgtccaaa gaccaatcaa agcgtccact tccgctcaag gcctataaaa
     1501 acctggactt tttaaacagc aacagtgccc gtgtgttgcg gattatgagc gaatatctgg
     1561 agccggaatc gagattccgt cattacaagg tcaaagacac catcgtcttt tttggttcag
     1621 cccggattcg cccgcgtgag gaggttgaga aagaattaaa caatgacggg cttgaagatg
     1681 gccagcgaga caaactggat cgggccttgg agatgagtcg ctattatgat gatgcagttg
     1741 aacttgctcg gctgttgacc gaatggtcaa aaagtctcaa agaacgccag cggcggtttt
     1801 tgatttgttc cggaggcggg ccaggcatta tggaagctgc caatcggggc gccgcactgg
     1861 ctcatggcaa gtcaattggc cttaatatca gccttccgtt tgagcaaacc ccgaacccat
     1921 acatttcaaa tgagttgagc tttgagtttc actacttttt catgcgcaaa ttctggttta
     1981 tctatctgtc aaaagcactg gtgattttcc ccggcggctt tggaacgtta gacgaactcc
     2041 ttgaagtcct caccctgatt caaaccaaaa agctccgccg cccgattccg attgtgattt
     2101 atggtgaaca gtactggcgt gaagtcttga atcttgacgc ccttgaaaag tgggggatga
     2161 tcaataaaga ggacctgaag ctgtttcgtt ttgccgatac gccacaagct gcctttgaat
     2221 acctccggga tcaattgcag atgttctatg gcgaagaggc cgaagcctca ccgtttaatg
     2281 aataaaaccg ggctgaagac atcgggctga agacatcggg cttggggcaa tcgaagggat
     2341 gagggatgaa ataaacccaa ttcttcagcc ctcagcccca tgtcttcagc cctcagccct
     2401 cagcccttag ccctggtttt ctcagcccaa agaacaaaaa gccacccatg caaatcactt
     2461 ttcttggtac cagttcaggc gttccaactc gggcacgtaa tgtgtcgagc gtgatcctgt
     2521 cgttgactca acgaggggag cagtggatgt ttgactgtgg cgaaggcact cagcatcagt
     2581 tttttcgcag caacctcagt atcagtcagt tgcgccgcat tttcgtcacc catatgcacg
     2641 gggaccacac ctttggactg atgggcttgc tttccagcgc tggtctgacc ggacgaatga
     2701 ccaaggttga tttatatgga ccggctgagt tgaaagactt gatccacgcc tgccagggct
     2761 tcacacagac ccagtttccc tacaatctca atattcaccc ggtgaaacct ggactggttt
     2821 ttgaagacca cgaatatacg gtcagttgtg ccacgctccg gcatcgcatt ccggcctatg
     2881 gctaccgggt gcaggaaaaa gaccggcccg gccagtttct ggtcgaaaaa gcaaaggcac
     2941 tggggattcc gtttgggccg ctctacggag aactcaaacg cggcaacacc atcacgctcg
     3001 ccgatggccg aatcatcaat ggcgctgaac tctgtggtcc acccaagccg gggcgatcag
     3061 tcgtgtattg caccgacacg atttattccg aatcatccgt ccggctggca aaagacgctg
     3121 atgttttgat tcatgaaagc acctatgccc acgaagacga acccctggcc caccgggcac
     3181 tccactcaac cacgcgcatg gccgctcgtg tcgccctcga agccggtgta aaacagcttc
     3241 tattgacaca tttcagcccg cgctacacca atgaaagcgc gatcaccctg gatgatttgc
     3301 tcaaggatgc gcgttcgatc tttccgaata ctgaactggc gcacgatttt ttggtgtgga
     3361 aggtagataa ataggggata gggggtaggg gatagggaaa tccaatattt tcaatgattt
     3421 cgccttttgt agtcagtagc ccgtagtcag tagttcgcta actccatttg attgaattac
//
LOCUS       DAOQDO010000387         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20273_length_3472_cov_3.171495, whole genome shotgun sequence.
ACCESSION   DAOQDO010000387 DAOQDO010000000
VERSION     DAOQDO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20273_length_3472_cov_3.171495"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="PKZ53_07515"
     CDS             complement(<1..1451)
                     /locus_tag="PKZ53_07515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ40320.1"
                     /translation="MSLGTIDQPSGTVLDGKYRLEAPIGLGGFGIVYRAIHLGLGRAV
                     AVKILHANVSNATAEELERFRLEGVSTCRIDHPNAISILDFGITADGFAYLVMELLDG
                     RLLVDELREKGSLSLQRATEIIVPVCDVLAEAHSAGIVHRDIKPDNIYLHQTKRGEVV
                     KVLDFGIAKLSGSAANVSGRSLTGAGIVMGTPEYMAPERVRNKPYDGRSDVYSLGIMM
                     YQMLSGRLPFESQDGDLVAVMWMQANDPPPSLRQYVPEIPIEIESLIFRALSKQSTLR
                     PTAEEFADELIRITHHQRQLFLSSQVASPASESSASAEKSSEADPVMFLPQPAAVQTH
                     SGQITQPTLHSPPTAPILLEPATVRTEIPSQLTPNLPSRVVLFDVPPTMPPVPDHTAP
                     LVSPVPIPSEPVPNFNKTTEETVFPISFTGPWDAKQFEDQLFAVVDSSDTIQAVQESD
                     LESLENQSPEPAPVPEPPPILVQVTGFQGTPGL"
     gene            1716..1919
                     /locus_tag="PKZ53_07520"
     CDS             1716..1919
                     /locus_tag="PKZ53_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40321.1"
                     /translation="MTKLMMVAVIVMSWNFSVQAQTGIKLPKGIPVLVDGQVATGEWD
                     AATEIKISDQITLAAKVSGDFIF"
     gene            1986..2279
                     /locus_tag="PKZ53_07525"
     CDS             1986..2279
                     /locus_tag="PKZ53_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40322.1"
                     /translation="MVNLHASAKLGERELKDGTWPDWVWWNNRGWVANTSRGIAFERR
                     EFLPDEIKEFQIRRSRFPGKVWRVRLEVHTNRTIGFPEKAEPNNPETWLKFKL"
     gene            2382..>3472
                     /locus_tag="PKZ53_07530"
     CDS             2382..>3472
                     /locus_tag="PKZ53_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ40323.1"
                     /translation="MSWFDVLFGKQLASHEEEEQRVGTIAAIPMLGLDALGSAAYGPE
                     AALTLLLPLGVLGLSYILPITLLIVFLLFVVYLSYRQTIAAYPTGGGSYTVARRNLGV
                     KFGLLAAAALLLDYVLVVAIGISAGVGALVSAFPSFQPYTLTLCLAILGLIAFVNLRG
                     VRESGMAFILPTYLFVTMMFVVIAIGIVKTLASGGHPTAVAPTPHLHATLGTASLWLL
                     IHSFSSGCTAMTGVEAISNGVMAFQEPRVVKAQRTLTAVIIILTVLLIGIAFLVRAYG
                     IGATEPGSKGYESVLSQLVAAVIGRGFFYYVTIGAILSVLALSANTGFADFPRLCHII
                     AQGGYLPYSFASRGRRLVYSQGIYVLTALA"
BASE COUNT          784 a          836 c          984 g          868 t
ORIGIN      
        1 atcagacctg gagtcccttg aaaaccagtc acctgaacca ggatgggtgg aggctcaggt
       61 actggtgctg gctcaggtga ctggttttca agggactcca ggtctgattc ctgaacggcc
      121 tggatggtgt cggacgaatc aaccaccgca aaaagctggt cttcaaattg tttggcatcc
      181 cacggcccgg tgaaggaaat cgggaagacc gtctcttcgg tggttttatt aaagttcggg
      241 actggctctg aggggattgg cactggcgag accagcggcg ccgtgtgatc aggaactggg
      301 ggcatggtcg gagggacatc aaacaacacg acccgcgatg gtaaattggg ggtgagttga
      361 gatgggatct cggttcgaac cgtggccggc tccagcaaaa tcggggcggt tggcggcgaa
      421 tgaagtgttg gttgggttat ctggccagag tgcgtttgga ctgctgctgg ctgaggtaaa
      481 aacataaccg ggtccgcttc agacgatttt tccgctgaag ctgaagattc agaagccggg
      541 ctggccacct ggcttgacaa aaacaactgt cgttgatggt gcgtgatccg gatcagttca
      601 tccgcaaatt cttcggctgt tggtcgcaac gttgattgtt tcgagagggc gcgaaagatc
      661 aaagattcaa tctcaattgg gatttcagga acgtactggc gcaacgacgg cggtggatca
      721 ttggcctgca tccacatcac agcgaccaga tctccgtctt gtgattcaaa aggaagccgg
      781 ccactgagca tttgatacat cataatcccc aggctataga catcagaccg tccgtcatag
      841 ggtttgttcc gaacgcgctc aggcgccata tactctggcg ttcccatcac aatcccggcg
      901 ccggtcagac tccggccact gacgttggcg gctgaacccg agagtttggc aatcccaaaa
      961 tcgaggacct tcaccacttc gccgcgtttg gtttggtgga ggtagatatt atctggcttg
     1021 atatcgcggt gaacaatccc ggcagaatgg gcttcggcca gcacatcaca gactggaacg
     1081 atgatttctg tcgcccgttg aagagaaagg ctgccttttt cgcgcaattc atcaaccagc
     1141 aatcgtccat cgagcaattc catgaccaga taggcaaagc catcagcggt gatgccaaaa
     1201 tcgaggattg aaatggcatt tggatggtca atccggcagg tcgagacccc ttcgagtcga
     1261 aagcgttcca gctcttcggc ggtggcatta ctgacattgg catgcaggat tttgacggca
     1321 acggcccggc ccagcccaag atgaatcgcc cgatagacaa taccaaaccc acccaggcca
     1381 attggggctt caagacggta tttcccgtcc agcacagttc cagatggttg atcaatggtg
     1441 cccagtgaca tcgaactcac ccaacgaaga caacaagaaa atcaaaacca gtgtgcctgg
     1501 tcaaggtgca ggtggttttg atttgggatt tcctggaaaa tgattcggag gaccggatct
     1561 tcattctgaa tcacagatgg cggttgaaac aagcgaaaag tgacgtgctc cgggttccag
     1621 gtgcttgatc tccttcgggt ctgtggttgc gcgtcaatga taatcagttt tccggttctg
     1681 gttttccacg tcaaatcaac agagggaagg aacttatgac gaaattgatg atggttgcgg
     1741 tcattgtgat gagctggaat ttctcagttc aggctcaaac cgggatcaag ctgccaaagg
     1801 gaatcccagt tttggtggat ggacaggtcg cgactggtga atgggatgct gccacggaaa
     1861 tcaaaatttc cgatcaaatt accctcgctg caaaagtcag cggtgacttt atcttttgag
     1921 ctgtcaaata tcccaaccca acctattttg gggttgatct ctatgtcaag tcggcgaata
     1981 gtcagttggt gaatctccat gcctcggcca aacttggtga acgcgaactc aaagatggaa
     2041 catggccgga ctgggtgtgg tggaataacc ggggctgggt ggcgaacact tcgcggggga
     2101 ttgcttttga acggcgggaa tttctcccgg atgaaatcaa agaatttcaa attcgccgct
     2161 cacggtttcc agggaaggtc tggcgggtac gacttgaggt tcacaccaat cgaaccattg
     2221 gctttcctga aaaggctgag ccaaataatc cagagacatg gttgaagttc aaactgtaga
     2281 tctgaacaga tcaaggcacg atgtcggtga cgcgctggca taaatggtaa tttttttgag
     2341 gactcgcttt tataaaaatt taaaaattgg agacacaatc catgtcctgg ttcgatgttc
     2401 tgtttggaaa gcaacttgct tcgcatgaag aggaagagca acgagttgga accatcgctg
     2461 ccattccaat gctgggtctg gatgcccttg ggtcggctgc ttacgggccg gaagccgcct
     2521 tgacattgct cttaccgctc ggggttctcg gcctgagtta catcctgcca attaccctgc
     2581 tgatcgtttt tttgttgttt gtggtgtatt tgtcataccg gcagaccatt gctgcctatc
     2641 cgactggcgg tggtagctac acagtcgccc ggcggaatct gggggtgaaa tttggcttgc
     2701 tggcagccgc agccctcttg ctggactatg ttctggtggt ggctatcgga atttcagccg
     2761 gagttggggc gctggtttcc gcctttcctt cgttccaacc atacacattg accctctgtc
     2821 tggcgatttt ggggttgatt gcctttgtga atctgcgagg cgttcgcgaa tcaggaatgg
     2881 cgtttatcct cccaacctac ctgtttgtaa ccatgatgtt tgtggtcatt gcgattggga
     2941 tcgtaaaaac gctggccagc ggaggccatc cgacggcagt tgctcccacg cctcacctcc
     3001 acgcgaccct gggcacggcg agtttatggc tgctgattca ttcattttca agcggatgca
     3061 cggcgatgac gggcgttgaa gcgatttcaa acggtgttat ggcgtttcag gaaccgcgag
     3121 tcgtcaaggc gcaacggacc ctgaccgccg tcattatcat tctgaccgtt ctgctgattg
     3181 ggattgcctt tctggtgcgg gcatatggaa tcggtgcgac ggagccgggt tcgaagggct
     3241 atgaaagtgt actgtcgcaa cttgtggcag cggtcattgg acgcggtttt ttctattatg
     3301 tgacgattgg cgccattttg agtgtgctgg cactttcagc caacactgga tttgctgact
     3361 ttccccgact gtgccacatc attgcccagg gaggctatct gccctattcc ttcgcctcgc
     3421 gtgggagacg actggtgtac tcgcaaggaa tttatgtctt aacggcgctg gc
//
LOCUS       DAOQDO010000388         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20313_length_3469_cov_2.625952, whole genome shotgun sequence.
ACCESSION   DAOQDO010000388 DAOQDO010000000
VERSION     DAOQDO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20313_length_3469_cov_2.625952"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..3009
                     /locus_tag="PKZ53_07535"
     CDS             460..3009
                     /locus_tag="PKZ53_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ40324.1"
                     /translation="MSSSRSSQPLSESSSALVAQRGSFASFLAPLTQDTFKQVISSIQ
                     GNLEVVYQTLSWLEGFQDHQGFELLLDEMLQAITLKIGELLNADRTTIFLLDEEKDEL
                     WSIVAKNENGNLLEIRLPTTVGIAGEVACFKKAINIPFDFYADPRSATARRFDQRHNY
                     RTYTMLALPLLNEAGDLVAVVQLLNKLKPGSEPFSSLAERIDLAGFTETDEETFQEFA
                     PSIKLILESSRLFYTAAQKQRAATALMKATQSLSQSSLDLEETLKRVMDEAKELLKAD
                     RSAIWLLDEESRQLWTKLPIGGVLQEIRIPASAGFAGQVATTGEPVNIGFDLYAHSNS
                     ETSKQTDQRTGYRTCSLLCMPIFNANQKLIGVTQLINKKKQGEFPPYDAARWPEPPEC
                     WKASFTRDDQEFMQAFNIQAGVALENAKLFATVKQQEQMQRNILRSLSNAVIATDHAG
                     RVMAVNESARHLLNLKPEDHIEGQCLSELIHLKENDFATWFGKALEPQTGKPLEQYYP
                     EQILMLDAQSDSHSVNLTINTIADATDPNLVRGALVVMEDISQEKRLKNTMYRYMTQE
                     LAERLMQNSDSAKLGGDRKDVSVLFSDIRGYTTLTEHMPPEEVVEMLNEYFEQMVEAI
                     FRFKGTLDKYIGDAIMAVFGSPLQLEDHAWYAVQAAIEMRHRLVEFNTRRKAHQLNTI
                     AIGIGINTDSVISGNIGSSKRMEFTAIGDGVNLGSRLEGASKQYGCDIIISDRTYLAC
                     EQRVWARELDCIRVKGKTEPVSIFELIGIREGPLADQVSEHQLKIIEYYQRGLELYRQ
                     REFTQALREFNRVLQIAPNDKPALMHKDRCWHLISAPPAQSWDGVWTMTEK"
     gene            3216..3341
                     /locus_tag="PKZ53_07540"
     CDS             3216..3341
                     /locus_tag="PKZ53_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40325.1"
                     /translation="MNNVPAKVLNASVEGLNLQPGTLRDQLDQQGTLLLFLRHFG"
BASE COUNT          904 a          855 c          878 g          832 t
ORIGIN      
        1 ggtcaaattt gtcgagccac aacaggggcg tgatgaatta gaaaaattga cccgacccgc
       61 cgaaattttt gctgtcaacc tcaaacccat gtgccgaacg ctggtcgcca gggaagtcaa
      121 tgaactggac ctgccaaatt tgcgagtggc acaattagat gtggactatg aatggtaatc
      181 tgggttgccc cctcccacag ccaatctgct cagataaagg attgaaaccc aatgctgacg
      241 aaggggacag ggagacactg tttgagagga aggggggccg gtacctgggt aagattgaac
      301 tcaaaacgag tttttgaagg ccaatcaacc caatgaaaag aaggaattgc aggttgtgac
      361 ccctgttgcc cggctctgat ccccggttct ctctgctgct tgccgtcttc accccttcct
      421 ctttggtcag ttcccaaatg cttccttcaa tcacgaaata tgagttcttc ccggtcttcc
      481 caacctcttt cggaatcctc ctcagctctg gtcgcccaac gtggaagttt tgcttcgttt
      541 ttagcgccac tgactcagga tacgtttaaa caggttattt cttcgatcca gggcaatctc
      601 gaagtggttt accagaccct ttcctggctt gaagggtttc aggatcatca gggctttgag
      661 ttgctgcttg atgaaatgtt gcaggccatt accttaaaaa tcggtgaatt gctcaacgcc
      721 gaccgcacca caattttttt gctggatgaa gaaaaagacg aattgtggtc catcgtggcc
      781 aaaaacgaaa atgggaactt gctggaaatc aggttgccca cgacggtcgg gattgctggc
      841 gaagtcgcat gctttaaaaa agcgatcaat attccgtttg atttttatgc tgatccacgc
      901 tcagccacgg cacggcgatt tgaccagcgg cataactatc gaacctacac gatgctggct
      961 ctgccgctgt taaacgaagc gggcgatctg gtggctgtgg tccagcttct caataagctc
     1021 aaaccgggga gcgaaccatt ttcatcactg gctgaaagga ttgatctggc cgggtttacc
     1081 gaaaccgatg aagaaacatt ccaggaattt gccccttcga tcaaattgat tcttgaatca
     1141 tcgcggctgt tttacacagc ggctcagaag cagcgggcgg caacggcact gatgaaggcc
     1201 acccagtcgc tcagccagag cagcctggat ctggaagaaa ccctcaagcg ggtgatggat
     1261 gaagccaaag aactcttgaa ggctgaccgg agcgccatct ggttgctgga tgaagaatcc
     1321 cggcaactct ggaccaaact tcccattggt ggtgtgttgc aggaaatccg cataccagcc
     1381 agtgccgggt ttgccgggca ggttgcaacc acgggggagc cggtcaatat tgggtttgat
     1441 ctctatgccc actctaattc cgaaacctca aaacaaaccg atcaacggac cgggtatcgg
     1501 acctgcagcc tgttgtgtat gccgatcttt aacgccaatc agaaattaat tggcgtgaca
     1561 caattgatca acaagaaaaa acagggtgaa tttccgccct acgatgctgc ccgatggcca
     1621 gagccgccag aatgctggaa ggccagtttt acccgtgacg atcaggaatt tatgcaggcc
     1681 ttcaatattc aggccggggt ggcgctggaa aatgccaaat tgtttgccac cgtcaagcaa
     1741 caggagcaaa tgcagcgcaa tattttgcgc agtctctcaa atgcggtgat tgccaccgat
     1801 catgctggtc gggtgatggc cgtcaatgaa agtgcccgac acctgctcaa cctcaaaccc
     1861 gaagaccaca tcgagggaca gtgcctgtct gaattaattc atctcaaaga gaatgacttt
     1921 gccacctggt ttggcaaagc gcttgagcct cagaccggga agcccttaga gcaatattat
     1981 cccgagcaaa ttctgatgct cgatgcccaa tctgattctc acagcgtcaa tctcacgatc
     2041 aataccattg ctgatgcgac ggatccaaat ctggttcgag gcgcactggt ggtcatggaa
     2101 gatatcagcc aggaaaaacg cctcaaaaac accatgtacc ggtatatgac ccaggaactg
     2161 gccgagcgtt tgatgcaaaa cagtgacagc gccaaactgg gtggggatcg caaagatgtt
     2221 tcggtcctgt tttcagatat tcgggggtac acaaccctca ctgaacatat gccgcccgaa
     2281 gaggtggtcg aaatgctcaa tgaatatttc gagcagatgg tggaggcaat ttttcgattt
     2341 aaaggcacat tggataaata catcggcgat gccattatgg ccgttttcgg gtcgccgctt
     2401 cagttagaag accacgcctg gtacgcggtt caggctgcta ttgaaatgcg tcaccggctg
     2461 gttgagttca ataccagacg caaggcccac cagctcaaca ccatcgccat tggaattggc
     2521 atcaataccg acagtgtcat cagtggcaat atcggttcca gtaaacgcat ggaatttacc
     2581 gccattggcg atggggtgaa tttaggttcc cggctcgaag gtgccagcaa acaatatggg
     2641 tgcgatatta tcatcagtga tcgaacctat ttggcctgtg aacagcgggt gtgggcccgt
     2701 gaactggact gcatccgggt caaaggaaaa accgagccgg tttcaatctt tgaattgatt
     2761 ggaatccgtg aaggaccact ggccgatcag gtatcagaac atcagttgaa gattattgaa
     2821 tattatcaac ggggacttga attatatcgc caacgcgaat ttactcaggc tctgcgggaa
     2881 tttaaccgtg ttctccagat tgcccccaac gataaacccg cattgatgca taaagatcgc
     2941 tgctggcatc tcatctcggc tcccccggct caaagttggg atggggtgtg gaccatgacg
     3001 gagaagtaag aatagacccg agtcagtagt cagtggtcag tcatgagtag tcaacaggtc
     3061 actaactcca gcttcttgaa ttgcttaact gcttgttcat atcagccatt cattccagga
     3121 tagtataaaa atcacctgtc tcccgttgga taattgaacc agagcgacct gattttgttt
     3181 caaccgcaac ttccagacaa aggatttcag accctatgaa caacgtacct gccaaagtgt
     3241 tgaatgcgtc agtggaagga ctcaacttac agcctggaac gctccgggat cagttggatc
     3301 aacagggcac gctcttgttg tttctccgtc attttggctg agtgttccca cgtgagagcg
     3361 taaaggattt acgccagatt atcgctacgc aggccgacta tcctccgatt gtatttgtcg
     3421 tgcagggctc agttgaagaa ggaaaagcct tttttgaagg actctggcc
//
LOCUS       DAOQDO010000389         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20374_length_3464_cov_3.484600, whole genome shotgun sequence.
ACCESSION   DAOQDO010000389 DAOQDO010000000
VERSION     DAOQDO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20374_length_3464_cov_3.484600"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="PKZ53_07545"
     CDS             complement(<1..1841)
                     /locus_tag="PKZ53_07545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNJ40326.1"
                     /translation="MYHRTRRTVYVVLLLVAICGAVMVNPFGNTTASKPQSQRQKPTS
                     SASLPGQQPSTNQAQGSIWPSLEKQLVNARAPKGSALERLIRENQDFAELDPSEVNDT
                     LRVPLFLRVIWRKAHPESRQLANDPTKGYPFVLKEVFEWMQSHPDLLPGTPEPPVAPS
                     DEVRAPVIGTNLRISGSQTVPQSESDIRINFFDPTKVIAASNNIGGSGMQSQFYSTNS
                     GATWGQTSLSLFSGDSFHSDPTVDWTSDGRAWSTTLGINGAGSQLRGRSYVSTNNGAA
                     WTQDGTFSGSQTQVDKQMVWIDHSSSSSFQNNQYVIWHNGAPVFMNRRVSAWGTPIQV
                     SGSETTGTGIGSDVKTNAFGDVFGFWPDTGSKGLYVVKSTNGGTSYSTPIQVATTFDS
                     YDIGVPGFNSRRVLIYVSGGAYRTAVKNNVYLTWSDLDAAGSEPGSNASSARKTRIWF
                     SRSTNGGTSWSAKAMINNQAGLNDQFNQWMAVDETSGAIGVIYYDTVGDSTRKTVNIW
                     YQASYDDGVTWEVPVKVTTAVTDETASGADSGNQFGDYNGLSGFSGVFFPSWTDRRNN
                     AREEIWTSKITDNAVPSPSVIAAGSSITNGNCGSPATTAIDPGETVTV"
     gene            2397..3410
                     /locus_tag="PKZ53_07550"
     CDS             2397..3410
                     /locus_tag="PKZ53_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40327.1"
                     /translation="MKLRHICTMSILFFFLVIQAGCLDFEEKIDEGVVIADKVEVISS
                     TALIHTKLGQVKKGDRLDISDRINANGIDYVKIRFQNPQGRKQYGWIESRHIISQKIL
                     ARCQELLQKEGENLPTQIQGKLKVQSNLRLSANRDAEVVVLLRSETAFDVIKHLRVEE
                     KLVKSNSDDAAEQPDDDDADTRFVDWYLVRFPKESIFPGGWVYGPAMELIAPEPLPSL
                     VGSGRRVTGCVPIGSTKDKDGVLQTNYIVLDKSTFNRDSSIDFDRIYIVEWDPKQEEY
                     TSIYREPDVRGTYPILYQVGDGRTEFTVSLLNKQNQPVRTRYEIRRDFSSGFLQVKKV
                     GGA"
BASE COUNT          886 a          792 c          917 g          869 t
ORIGIN      
        1 accgtcacgg tttcgcctgg gtcaatcgcg gttgtcgctg ggcttccaca atttccgttg
       61 gtgatgctag aaccagccgc aatcacagac ggtgatggaa ccgcgttgtc agtgattttt
      121 gaggtccaga tttcttcccg ggcattgtta cgacgatccg tccagcttgg gaaaaagacg
      181 cctgaaaatc ctgaaagacc gttgtagtcg ccaaactggt tgccggaatc ggcacctgac
      241 gccgtttcat cggtgacagc cgtggtgact ttaaccggga cttcccaggt tacaccatca
      301 tcataagacg cctgatacca gatattgacg gttttacggg ttgaatcgcc caccgtgtca
      361 taatagatca cgccaatggc accagacgtt tcatcaactg ccatccactg gttgaactga
      421 tcgtttaagc ctgcctgatt attgatcatg gctttggctg accagcttgt tccgccgttg
      481 gtcgagcgtg aaaaccagat gcgggttttg cgggccgatg aggcgttgga gccaggttcg
      541 gaaccggctg catccaggtc tgaccaggtt aagtagacat tatttttgac ggcagttcga
      601 taggcgccgc ctgacacata aatcaggact cgccgactat tgaagccagg aacaccaatg
      661 tcatagctgt caaaagtggt ggcaacttga atcggtgtgc tatatgaagt tccaccattg
      721 gtggatttca caacatagag gcccttactt cctgtatccg gccagaaacc gaacacatca
      781 ccaaacgcat tggttttgac gtctgaaccg atacctgtgc cagttgtttc actgccactc
      841 acctggattg gggttcccca ggcgctgacg cgccggttca taaaaactgg cgctccattg
      901 tgccagatca catattggtt attttgaaag gaagaggagc tgctgtggtc aatccacacc
      961 atctgtttat caacttgagt ttgagaaccg ctgaacgttc catcctgcgt ccaggcggca
     1021 ccattgttgg tcgaaacata actgcgacct cgaagttgag atccagcacc attaatgccc
     1081 agtgtggttg accaggcccg accatctgag gtccagtcca cggttgggtc agagtggaac
     1141 gaatcacctg aaaacaggct gaggctggtt tgtccccagg tcgcaccact gttcgttgag
     1201 taaaactggc tctgcatacc gctgccgcca atattgttgg aagccgcaat tactttggtt
     1261 ggatcaaaga aattgattcg gatatctgat tcgctttgtg gaacagtttg agaaccagag
     1321 attcgcaaat tggtgccaat cactggcgcc cgtacttcgt ccgagggggc tactggtggc
     1381 tctggggtgc cagggagcaa atctgggtga gattgcatcc attcaaacac ttccttcagc
     1441 acaaacgggt agccctttgt cggatcattg gccagttgac ggctttccgg atgggctttg
     1501 cgccagataa cgcgcaaaaa caacggaact cgaagagtgt cattgacttc agacgggtca
     1561 agttcggcaa aatcctggtt ttcccggatc agccgttcca gtgcgctccc tttcggtgcc
     1621 cgcgcattga ccagttgttt ttccagtgat ggccagattg aaccctgggc ttgattggtt
     1681 gacggttgtt ggcctggaag ggaagccgaa gatgttggtt tttgtctttg ggattgtggt
     1741 ttggaggcag tggtattccc aaacgggttc accatgaccg ctccacaaat ggcaacaagc
     1801 agtagcacga catagaccgt gcgtcgggta cgatggtaca tgggcaattt cctctttgga
     1861 tcgaacaaag caggccgcaa cgggcccaca gcaaaagcgt tgaatttgtt tgttatgaaa
     1921 agcagtgatg cttgacgcaa aatgcaacta gagactggtc tgacgccagc ttaattttgg
     1981 aaaaagcggg taaattggat ctggcacagt gctggtgttt tcttgtaggg gatcagatgg
     2041 ctgaggaccc aaacaagaca aacccaccag gacaaacgtg ggcattggaa aacattgaaa
     2101 atgtgttggt atggatgaga ggcaggcagg atcccccctg ggtcaaaaga aagtgcaaac
     2161 tgtagcagcc tggtattaag tggatcaagt caaaatgaag aagttcagtt tttatcacgg
     2221 tttaactggt tttctcaatg ccaaagaggt gtgtcgctgg caacagatgg tgtcagtcca
     2281 tttgattttt ccctgatcga aatttgtctc actggacaat cccgtgttat aaggggttgc
     2341 cagcctgcat acatctggtc tccgtcactt ttatcccagt tcaaacgaga agacgcatga
     2401 aacttcgcca catctgcacg atgagcattc tgtttttttt tctagtcatt caggctggtt
     2461 gtctggattt tgaagagaaa attgatgaag gggtcgtcat tgctgataag gtcgaggtca
     2521 tcagttcaac ggcgttgatt cacaccaaac tggggcaggt gaaaaaagga gatcggctcg
     2581 atatctctga ccgcatcaac gcgaacggaa ttgattatgt caaaatccgg ttccagaatc
     2641 cccagggccg taagcaatat ggctggattg aaagtcggca tatcatcagt cagaaaatcc
     2701 tggccagatg tcaggaattg ctccaaaaag agggcgaaaa cctgcctacc caaatccagg
     2761 gaaaactcaa agttcaatcc aatcttcgtc tgtctgccaa ccgggacgct gaagtggtgg
     2821 ttttgctgcg tagtgaaacc gcctttgatg tcatcaagca cctacgagtt gaggaaaaac
     2881 tggtgaaatc caactcagat gatgcggctg aacagcctga tgacgatgat gctgataccc
     2941 ggtttgttga ctggtatctg gtccggtttc cgaaagaatc catttttcca ggtggttggg
     3001 tatatgggcc ggcgatggag ttaattgcgc ctgagccact tcccagtttg gttggttcgg
     3061 gccgacgcgt cacagggtgc gttccgattg ggagtaccaa agacaaagac ggtgtgctgc
     3121 aaacaaatta tattgtcctc gacaaaagca cgttcaaccg cgatagctca attgactttg
     3181 atcggattta cattgtcgaa tgggatccaa aacaagagga atacaccagc atctatcgcg
     3241 agccagacgt tcgcggtacg tacccgattt tgtatcaggt tggagatggc cggaccgaat
     3301 ttactgtgtc tttactcaat aaacaaaacc agcctgtgcg gaccagatat gaaattcgac
     3361 gtgatttcag tagcgggttt ttacaggtga agaaagttgg cggcgcgtaa agaattgaag
     3421 aatgaagaat gaagaatgaa gaatgaagaa tgaagaatga agaa
//
LOCUS       DAOQDO010000390         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20410_length_3460_cov_3.922761, whole genome shotgun sequence.
ACCESSION   DAOQDO010000390 DAOQDO010000000
VERSION     DAOQDO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20410_length_3460_cov_3.922761"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /gene="glpK"
                     /locus_tag="PKZ53_07555"
     CDS             complement(<1..1381)
                     /gene="glpK"
                     /locus_tag="PKZ53_07555"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNJ40328.1"
                     /translation="MSSQKYILALDQGTTSSRAILFHQHGQPVGISQRPFEQIYPKPG
                     WVEHRPDDIWSSQLGAARDLLRQHHLTPADIAAIGVTNQRETTIVWDRKTGQPIHNAI
                     VWQCRRTADRCDSLRQSHHKALIQEKTGLIVDAYFSASKLEWLLDQVPQARQRAEAGD
                     LAFGTVDTWLIWNLTGGRVHATDVSNASRTMLFNIHTAQWDDELLKLFNIPRSLLPAV
                     VPSSLVIGESDPQLFGGGIPISGVAGDQQAALFGQICTSPGLAKNTYGTGCFMLMNTG
                     TQAVTSNHSLLTTVAWKLGESGELNYALEGSVFIAGAVMQWLRDGLQIIGNAAESDAV
                     AQTVADTGGVYFVPAFVGLGAPYWDQQARGTITGLTRGTTRAHLVRAGLESIAYQTRD
                     VLEAMSADAGISFAQLRVDGGAASNDFLMQFQADMLNVPVIRPKSTETTAAGAAYLAG
                     LAVAYWSDLG"
     gene            complement(1834..>3460)
                     /locus_tag="PKZ53_07560"
     CDS             complement(1834..>3460)
                     /locus_tag="PKZ53_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40329.1"
                     /translation="PNGTYDFVDGFYNSPDRNNFAPSVSVAWDIPGLGRQTVLRGGYS
                     LTYVNDEIIRAADNAASGNDGLQAGVSNTAISGLVSRDALGLIGSSLATPEFKVPRSY
                     LDNFNLDPTSAAFLVDPELVTPYYHQWNVSLERELSSNMSLAIRYVGNRANNLVRGVD
                     FNQLDVVSNGFAQDVLRARSNGFLALAQNGRFDPRFNPNIAGSQQLLVFPKLTAGGLL
                     TNTGTIQPLIQRGEAGTLAQVYFVNGLNGAVQFTPNPNIFVADVLLNSGFSNYHSLQI
                     EFRRRFSQGLQVQANYAWGKSLTDVGSSPNGGNDNQTRFSPLLDNAQPQLERSRADFD
                     IPHTFKTNFLYELPFGPGKKFNPGNRIARKFVEGFQLSSIITWQSGNNLSFLSGRGTL
                     NRGGRSTTTNTADTSLTNDQLKDLLGVFQNERGIFFINPSVIGRDGRAVAADGASPFS
                     GQVFFNPNAGSVGSLERLAFNGPQQFFWDFSVIKRTTITESTNIEFRAEFFNVLNRAQ
                     FFIGNQNINSTNFGRITSTFDPRVIQFALKLNF"
BASE COUNT          848 a          901 c          916 g          795 t
ORIGIN      
        1 ccccaaggtc agaccagtat gcaacggcca gtcccgccag ataagcggca ccagccgccg
       61 tggtttcagt tgattttgga cgaatgaccg gaacattgag catatcagcc tggaactgca
      121 tcaggaaatc attggacgcg gctccaccat caacccgaag ttgggcaaaa gaaattcccg
      181 catcggctga catggcttcc agtacatcac gggtctggta ggcaatggac tctagcccgg
      241 cccggaccag atgggcacgg gtggtgccgc gggtcagtcc agtgattgtc ccgcgtgcct
      301 gttgatccca gtatggcgcg cctagcccaa caaaagctgg cacgaaatac acgccaccag
      361 tatcagccac agtttgggcc acggcatctg attcagccgc gtttccaatg atttgcaggc
      421 catcccgaag ccattgcatc acggcaccgg caataaacac gcttccttcc agggcgtagt
      481 ttaattcccc agattcacct aatttccagg caacggttgt cagcagcgaa tggtttgagg
      541 tcacggcctg ggtcccggtg ttcattaaca taaaacaacc agtgccatag gtgtttttgg
      601 ccagtccggg gctggtgcaa atctgaccaa acaaagctgc ttgctgatcg ccagcaacgc
      661 cagagatcgg aattccccca ccaaagagct gtgggtcact ttccccaatc accaggcttg
      721 aaggcacaac cgctggtaaa agcgagcgcg gaatattgaa aagcttaagc aactcgtcgt
      781 cccactgtgc cgtgtgaata ttaaagagca tcgtccgact ggcattggaa acgtccgtgg
      841 catgaacccg gcctccggtc aaattccaga tcaaccaggt atccaccgta ccaaaggcca
      901 ggtctccagc ctcagctcgt tggcgcgcct gcggaacctg atcaagtagc cattcgagtt
      961 tactcgccga aaaataggca tccacgatca aaccggtttt ttcctgaatc agggctttgt
     1021 ggtggctttg cctgagagag tcgcatcggt ctgccgtacg gcggcactgc cagacaattg
     1081 cattgtggat tggctgaccc gtttttcgat cccagacaat cgtggtttcc cgctgattgg
     1141 tcaccccaat cgcggcaata tcggctgggg tgagatgatg ttgccgaagc aaatctctgg
     1201 cggcgccaag ctgggatgac cagatgtcat ctgggcggtg ttcaacccat cctggctttg
     1261 ggtaaatctg ctcaaacggt ctttgtgata ttccaaccgg ctgcccgtgt tgatgaaaca
     1321 gaatcgcacg ggaactggtg gtaccttgat caagcgccag aatgtatttt tgagaactca
     1381 tagggagata ccattcaggg ttcagggttc agggttcagg gtttgacatt cagggtttgg
     1441 ggtttggtat ttagggttta gggtttgaga aacgacataa aggacatcac ttcgaaaacc
     1501 cggaacccca aatcttgaca agatgacaag gtgacaaggt gatttctttc atccctcatc
     1561 cctcatccct catcccttcc ggaacctgga acccgcctat tcttggcatc ctttgatcag
     1621 aaatcaaccc aaaagaaaga tcaccctttc tgtaccagag cagaaaaaaa gcccgttgga
     1681 gaaaaccaac gggcttcaaa tttttttgga gacaacacgt tatttttgtt ttgatgttct
     1741 cggttttttc ctctccagca gtcccattgt tgagccaggg caacagaaac ttgagcaatc
     1801 aactcaagga gccgttcccc tgaactctca caattagaag ttcaacttca acgcaaactg
     1861 gatcacgcgt gggtcaaacg ttgaggtgat gcgaccaaag ttggtgctgt tgatgttctg
     1921 gttgccaata aagaactgcg cccggttgag cacgttgaag aattcagccc ggaattcgat
     1981 attggttgat tcagtgattg tcgtgcgctt gatcacgctg aaatcccaga agaactgttg
     2041 tggaccatta aaggccaacc gttccaatga acccactgaa ccagcatttg gattgaagaa
     2101 cacctgacca gaaaatggag acgcaccatc agccgccacc gcccgaccat cacggccaat
     2161 cacggatggg ttgatgaaga agatcccgcg ttcattctgg aagacgccca gcaggtcttt
     2221 gagctgatcg ttggtcagcg aagtatctgc cgtgttggtg gtggttgaac gaccgccacg
     2281 gttgagtgta ccacggcctg acaggaacga aaggttgttc ccactctgcc aggtgatgat
     2341 tgaactcaac tggaagcctt caacaaattt ccgcgcaatg cggtttcctg ggttgaattt
     2401 cttccctggg ccaaatggca gctcatagag gaagttggtt ttgaaggtat gtggaatgtc
     2461 gaaatcggcc cgtgaccgtt ccaactgtgg ctgtgcatta tcaagcaatg ggctaaaccg
     2521 ggtctggttg tcattgccac cattggggga tgaaccaaca tcggtcagag atttgcccca
     2581 ggcatagttg gcctggactt gcagtccctg tgagaagcgg cggcggaatt cgatttgcag
     2641 tgagtgatag tttgagaacc cgctgttgag cagcacgtcc gcaacaaaga tgttcgggtt
     2701 tggcgtgaac tgaacggcac cgttcaatcc gttaacaaag tacacctgag ccagcgtacc
     2761 ggcttcgcca cgctgaatca gcggttgaat cgtaccggtg ttggtgagca agccaccagc
     2821 cgtcagtttc gggaacacca gcaattgctg gctacccgcg atatttgggt taaaccgggg
     2881 atcaaaccga ccgttctgag ccagggccag gaaaccattg ctgcgggcgc gcagcacatc
     2941 ctgggcaaat ccattagaaa cgacatcgag ctggttaaag tcaacgcctc gcaccaggtt
     3001 attggcacga ttgcccacat agcgaatcgc caggctcata tttgagctga gttcgcgttc
     3061 caggctgacg ttccactggt ggtagtaagg cgttaccagt tctggatcca ccaaaaaggc
     3121 ggctgaagtt ggatcaagat tgaagttgtc gagatagcta cgtggtactt tgaattctgg
     3181 ggtggccagt gatgaaccaa tcagacccag ggcatcacgg gacacaagtc cggaaatggc
     3241 ggtattgctg acaccagcct gcaacccatc atttccgctg gcagcgttgt cagccgcgcg
     3301 gataatttcg tcgttgacat aggtcagtga gtatccacca cggagaaccg tctggcggcc
     3361 caaccctgga atatcccacg ccacgctcac gcttggcgca aagttgttgc ggtctgggct
     3421 gttgtagaag ccatccacga aatcataggt gccatttgga
//
LOCUS       DAOQDO010000391         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20412_length_3460_cov_3.651101, whole genome shotgun sequence.
ACCESSION   DAOQDO010000391 DAOQDO010000000
VERSION     DAOQDO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20412_length_3460_cov_3.651101"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PKZ53_07565"
     CDS             complement(<1..129)
                     /locus_tag="PKZ53_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HNJ40330.1"
                     /translation="MPQNRVFRMKFAKVYPLYVQKAEHKRRTKEEVDQIICWLTGYT"
     gene            complement(153..650)
                     /locus_tag="PKZ53_07570"
     CDS             complement(153..650)
                     /locus_tag="PKZ53_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ40331.1"
                     /translation="MGTQLLLRDVVPDDLPIFFEHHLHPEANYMAAFTAKDRNNAEVF
                     MAHWHKILANEANIIKTIIVNGRVAGSVSSYQDEGKPEVTYWLGAEYWGQGIATWALR
                     EFLAHHNTTRPMYARVAKDNLGSRRVLEKCGFTLIGESVGFANARGREIEELVLVLGA
                     IADTM"
     gene            complement(1217..2182)
                     /locus_tag="PKZ53_07575"
     CDS             complement(1217..2182)
                     /locus_tag="PKZ53_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40332.1"
                     /translation="MQTRHYLKFAVLVCFLLPLFLVVSLQPVSAQQAREPYWEYVNTE
                     SGVVPYDPNGCYQPRIMIAQEGLVEITITHCGNPPEAQTTYRLEWTTPPQVIYPNKPF
                     DFKLKATLLANANPRWTLPGGLGISFTNFVWDPATQWGPISVGPQGNPHTAGETFIWD
                     NLSRPLAEQYAGPPQWWATDSRYAHPSRQIRLFIGVSASNKHWWSYVYRLVEPGKEPL
                     PQTGVFAGNWTGQWQNNLGEQGPDSLVLTEDELGNLRGTWSGKIAVSGRKLTATTGEL
                     QGQTATRNYQGTITLQGNVLTFTYVARRLNAAGGYEGRSRFTRAK"
     gene            complement(2450..>3460)
                     /locus_tag="PKZ53_07580"
     CDS             complement(2450..>3460)
                     /locus_tag="PKZ53_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40333.1"
                     /translation="YMLRPIACGRRIRVSVWVFVLFGSWMCGAFGFWAMIERVNFPIA
                     HASTLRYQPLSLDDRVMYQRKLEAVYWKHRLWPAENPNPKPSFDQVTTKADLRHQVET
                     TVRQSLVWQAVRHQPVTGEELQAELDRIASQSRDTELLQELFTVLDNDPAVIAECLVR
                     PLLVQRELGKIFEQQVTAESESSDQPKAAELTFDAWWTALASSRDIQEFASPHVAYRM
                     PDLSAARRFDPSATDCDQWSPISNDRGPFWTPNGGGSETAVWTGSRMIIWGSGKDYYN
                     PPYVSGGGEYNPVRDVWKPLPTENEPPGRSNHSAIWTGKEMIIWGKHKKGGQYRPCGS
                     TQ"
BASE COUNT          842 a          915 c          837 g          866 t
ORIGIN      
        1 ggtatagccc gtcaaccagc aaatgatttg atcaacttct tcttttgtcc gacgcttgtg
       61 ttccgccttt tgaacataca gcggataaac ctttgcaaat ttcatcctga aaacgcgatt
      121 ctgaggcatc atctttcctc ctgtggatcg ggtcacatgg tgtctgctat tgcgcccaat
      181 accaaaacca gttcttcgat ttctcgacca cgtgcattcg caaaccctac ggattcacca
      241 ataagtgtaa agccgcattt ttctaatact cgacgtgaac caagattatc tttggcgacg
      301 cgtgcataca ttgggcgtgt tgtattgtgg tgagccaaaa actccctcag cgcccaagtc
      361 gcaatcccct ggccccaata ttcagctcca agccaatagg tgacttctgg cttaccttca
      421 tcttgataac ttgaaacact tcctgcaact cgcccattga cgataattgt ctttatgata
      481 ttggcttcat tggccaaaat cttatgccaa tgtgccataa agacttctgc gttgttgcgg
      541 tctttggcag taaatgccgc catgtaattg gcttccggat gcaagtggtg ctcaaaaaag
      601 attggaaggt catcgggtac aacatcacgc aaaagcagtt gagttcccat aggttattct
      661 ccctgaattc acgtctatag tttgtcaggg cattttagta cactttcagc cgcctaacca
      721 ttgattaggt gtctcaatgg cacataacag gcgtatttgc catgttttcc gactttcggt
      781 catctaacat gcggattaag gatcttttca ggtttttgct gaatacaccg catcttaggc
      841 acaaaaaacg accacgacaa gcggttatcg ggttaaattg cacgaaatga gattcatctc
      901 agagggaatc agctcgtacc agtctgcagt cagtagctca gtcggagcaa tcctttgttc
      961 ggaaatgaag agaatgtcaa gagtcccacc ttcaggcggc accctgtcct tttagatcaa
     1021 atttgggaga caaacccgcc tgaaggcagc aacgcttcac tttttgaatc gcctcaggat
     1081 tccggagcgg tcttccaaaa tagcttatac cattttgcag tgaaacgcgc gcatcaggaa
     1141 acagctaaat cgctgagaaa attgcatttc cctgaacctt gaaccctgaa ccctgaaccc
     1201 tgaaccctaa tgggcattac ttcgcccgtg tgaatcgcga ccgaccctca tagccaccgg
     1261 ctgcatttaa tcgccgggcg acataggtaa acgtcaggac attgccctgg agggtaatcg
     1321 tcccctggta attccgggtt gcagtttgtc cttgtaactc accagtcgtg gcagtcagtt
     1381 ttcgcccact cacggcaatt ttccccgacc aggtgccacg caggtttccc agttcatctt
     1441 cggtcagcac cagcgaatcc ggcccttgtt cgccaagatt gttctgccac tgccctgtcc
     1501 agttgccagc gaacacaccc gtttgtggca gaggttcttt tccaggctca accagccgat
     1561 agacataact ccaccagtgt ttattggacg ctgaaacacc aatgaacagg cgaatctggc
     1621 ggctgggatg ggcataacga ctgtcggttg cccaccattg tggcggccca gcgtactgct
     1681 cagccagtgg acgcgataaa ttgtcccaga tgaatgtttc accggcagtg tgtgggtttc
     1741 cctgcgggcc tacggaaatt ggcccccact gcgtagcggg atcccagaca aaattggtaa
     1801 atgagatgcc gagacctcca ggcaatgtcc acctggggtt ggcattggcc agaagtgtag
     1861 ctttgagttt gaaatcaaag ggtttatttg ggtaaatcac ctgcggaggc gtcgtccatt
     1921 ccaaccgata ggtcgtttgt gcctcgggtg gatttccgca atgggtaatg gtgatctcga
     1981 ccagtccttc ctgggcgatc atgatgcgtg gttgataaca accgttcgga tcatagggca
     2041 cgacccctga ctcggtgttg acatattccc aatatggctc tctggcctgc tgggcactga
     2101 ctggctgaag actgacaacc aaaaacagcg ggagcagaaa acaaaccaac accgcaaatt
     2161 tcaaataatg gcgggtttgc ataaaagtgt acctcccaaa gccgacgttg aacgggtagc
     2221 cgttcaccca aatcgctttg attgggcgca cagcccctga ctgttggaga tgcaaagccc
     2281 aggccagcct ttacagggag gaaatcaccg tgaacatgtt gaaattccgc gatttaaggt
     2341 gttttttcgc aatctgtctg ggaaatatcg cggtgttggg gctcaaggcg taacactgaa
     2401 ccgaaatcat tttctgaaat gtgagggatc cgaatttctc aacccgaaat tactgggttg
     2461 agccgcacgg gcgatattgt ccgccttttt tgtgttttcc ccaaataatc atttcctttc
     2521 cagtccaaat agccgaatgg ttggaacgcc caggaggttc attctcagtt ggaagaggct
     2581 tccagacatc ccgaaccgga ttatattctc caccgccgct cacataaggc ggattgtaat
     2641 aatctttgcc actaccccaa atgatcatcc ggcttccagt ccaaacagcg gtttcagacc
     2701 cacctccatt tggtgtccag aacggccccc ggtcattact tattggtgac cattgatcac
     2761 agtctgtggc tgacgggtca aaccggcgag cggcagacag gtccggcatt cggtaggcaa
     2821 cgtgggggga ggcaaattcc tggatgtcac gtgacgaagc gagtgcggtc caccaggcgt
     2881 caaaggtcaa ttcggcggct tttggttgat cggaactctc agactcagcg gtcacctgct
     2941 gctcaaagat tttgcccagt tcacgttgca ccagcagcgg acgaacaagg cattccgcta
     3001 tcacggctgg gtcattgtcg agaacagtga aaagctcctg tagcagctct gtatcgcggg
     3061 actgactggc aatgcggtcc agttcggcct gcaattcttc tccggttacg ggttgatgac
     3121 gaacggcctg ccagaccaga gattgacgca ccgtcgtttc cacctgatgg cgaagatcgg
     3181 ccttcgttgt gacctgatca aatgacggtt tcggatttgg attttcggct ggccagagac
     3241 ggtgcttcca gtatacagcc tctagtttcc gctgatacat cacccgatca tcaagactca
     3301 agggctggta ccgtagagtt gaagcgtggg ctatcgggaa attcacgcgt tcgatcatcg
     3361 cccaaaaccc aaaagcgccg cacatccagc ttccaaacag gacgaacacc cacacggaaa
     3421 ctcgaatccg tcgcccacaa gcaattggtc ttagcatata
//
LOCUS       DAOQDO010000392         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20451_length_3456_cov_3.174949, whole genome shotgun sequence.
ACCESSION   DAOQDO010000392 DAOQDO010000000
VERSION     DAOQDO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20451_length_3456_cov_3.174949"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKZ53_07585"
     CDS             complement(<1..521)
                     /locus_tag="PKZ53_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNJ40334.1"
                     /translation="MKLKMFQVDAFTNRLFGGNPAAVVPLEDWLSEATMQAIAAENNL
                     SETAFIVPEGNDFGLRWFTPAVEVDLCGHATLATAHVIFTELDPSRKVVHFHTRSGKL
                     TVTRDGDQLSMDFPSRKPVPVEAPPALISALGAQPAAVLKSRDFFVVFETEAEVRALT
                     PDFPALGRLDTLG"
     gene            complement(564..1556)
                     /locus_tag="PKZ53_07590"
     CDS             complement(564..1556)
                     /locus_tag="PKZ53_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNJ40335.1"
                     /translation="MRKDTMSFAVGHPLPPVWMLVAAFAAVYIIWGSTFLAILFGLET
                     IPPFLMAGTRFLMAGGLLFGWAQLSGAGWPTLAQWRSAAVLGAAMLLIGNGAVVWSEQ
                     RIPTGITALLITTEPLWIVLLEWVMEKQRPTALTWGGLVLGTIGTIVLIGPGVFHGMG
                     AVDPLGAAAVTIGALSWAAGSLYSARAAMPASPTLSTGIQMLAGGVFLSIFSLMMGEW
                     THFEVAKVSLKSVLALGYLAVFGSIIAYTAYVWLTRVAPPSRVATYAYVNPIIAVLLG
                     WAVLNEHISLRTMCAAAMMITAVVLITLGKRTVAKQKIIEETTVDEKSLPEAAV"
     gene            1751..3190
                     /locus_tag="PKZ53_07595"
     CDS             1751..3190
                     /locus_tag="PKZ53_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNJ40336.1"
                     /translation="MEALTMQTQTHLYEQVAGKIERLIEDGVLRPGERVPSVRKVSQM
                     HRVSMATAFQAYFLLENKGLIESRPRSGFYVRMLSKGLPPTPNRTSPAAVATSVNVDD
                     LVGSILAAMRRPDLVQLGAASPSSDLLPVNRLNRTLMDVVRRSPKRTIEYELIAGNAE
                     LRRQIALRSLTWGGNLSADDIVVTNGCIEALNLCLRAVARAGDTIAIESPTYYGVLQV
                     IENLGMRALEIPTSPTDGICLDALETALHSQSIAACLVMSNFNNPLGSCMPDAKKQRL
                     VEILAGHVVPLIEDDIYGDLNFGTTRPKTCKAFDRDGLVLLCASFSKTLAPGYRIGWT
                     APGRFRAQVERLKGMNTIATATLPQMVVAEFLKNGGYERYLRQLRLHFASHLYAAKQA
                     IFQSFPPGTKVTRPGGGFVLWVEFPEGIDTLELHYRALEHKISLAPGVMFSPSKKYQN
                     CVRLSFGQKWSETTQRAIETVGKLASQGT"
BASE COUNT          824 a          933 c          915 g          784 t
ORIGIN      
        1 atgccgagcg tatcaagtcg cccaagcgcc gggaaatcag gcgtgagggc acgaacttcg
       61 gcttcggttt caaagaccac aaagaaatca cgggatttta aaactgcggc aggctgcgca
      121 cccagcgctg aaatcagggc ggggggagct tcgactggaa ccggttttcg ggatggaaaa
      181 tccatactga gctggtcccc atcgcgtgtg acagttaact ttccacttcg ggtgtggaag
      241 tgaaccactt tgcgactcgg gtcaagctcg gtgaaaatca catgggcagt ggccagcgtg
      301 gcgtgtccac acaaatcaac ttcaacggct ggtgtaaacc agcgtaatcc gaaatcgttg
      361 ccttcaggca cgataaacgc ggtttccgag aggttatttt cagccgcaat cgcctgcatg
      421 gtggcctcgc tcagccagtc ttcaagcggc accacggcgg ctggatttcc accaaacaac
      481 cgatttgtaa aagcatcaac ctggaacatt ttgagtttca tagatttcct cgtttagagt
      541 gcagcggcgt gacgccgcgc tggttacaca gcagcttccg gcagtgattt ttcatccacg
      601 gtcgtctctt caattatttt ttgcttcgct actgttcgtt tgccgagagt aatcaacacc
      661 acggctgtga tcatcatggc agccgcgcac atggttcgaa gtgaaatatg ttcgtttaac
      721 accgcccagc cgagtaaaac agcaataatc gggttaacat aggcataggt ggccacccgg
      781 ctgggtggtg cgactctggt cagccagaca taagccgtgt aggcaatgat agagccaaac
      841 accgccagat acccaagcgc cagcactgac ttgagcgaaa ctttggccac ttcaaaatgt
      901 gtccattcac ccatcatcag gctgaagatt gacaaaaaca ccccgcccgc cagcatttga
      961 atcccggtgc tcagggtcgg tgaggccggc atcgcggccc gtgccgagta caatgacccg
     1021 gcagcccagg acaaggcacc aatcgtcacg gcggcggccc ctaacgggtc aaccgcgccc
     1081 atcccgtgaa acacacccgg cccaatcagt acaatcgttc caatcgtacc cagcaccaga
     1141 ccgccccagg tcagggctgt tgggcgctgc ttttccatca cccattcgag cagcacgatc
     1201 cacaatggtt cagtggtaat gagtaaggcg gtgattccag ttggaatccg ctgttctgac
     1261 cagaccacgg caccgtttcc aatcagcaac attgcggcgc cgagtactgc cgcgcttcgc
     1321 cattgggcca gggttggcca gccagcaccg ctcagttgtg cccaaccaaa cagaaggcca
     1381 cccgccatga gaaatcgtgt tccggccatc agaaacggag ggatggtttc cagcccaaac
     1441 aggatggcca ggaaggttga cccccagatg atgtatacgg ctgcaaacgc agccaccagc
     1501 atccacaccg gcggtaatgg atgcccaacg gcaaatgaca tggtgtcttt tcgcatggtt
     1561 tttcaacttt cacgtgtgaa ctgttgagac tttaccttac ttgaagcgac cagaacagat
     1621 tcagttttgc catctttcca ccagatcagt tttgcgctag ggtatatgag tgctgcggct
     1681 tgacgcagct ttttggagtg cggtggcttg acaccgcttt taaatttgct ggtaaaagga
     1741 caccctcgcc atggaagctc tcacaatgca aactcagacc catttatatg aacaggttgc
     1801 cggaaaaatt gagcgcctga ttgaagacgg ggtgttgcgc cccggagagc gtgtcccgtc
     1861 agttcgcaaa gtctcgcaaa tgcaccgggt cagtatggca acggcgttcc aggcgtactt
     1921 ccttttggaa aacaaagggt taatcgaatc acgaccccgg tccgggtttt atgtccggat
     1981 gctctcaaaa gggttgccgc cgacaccgaa ccggaccagc ccggcggcag tggcaacatc
     2041 ggtgaacgta gacgatctgg tcgggtcaat tttggcagca atgcgccgac ctgatctggt
     2101 tcaacttggc gcggccagcc caagttctga cctgctgccg gtcaatcggc tgaatcgaac
     2161 cctgatggat gtggttcgtc ggtctccgaa acggacgatt gaatatgaat tgattgccgg
     2221 aaacgcagaa ctccggcggc aaattgcgct ccggtcgctg acctggggtg gcaatctgtc
     2281 agccgacgat atcgtggtca ccaacgggtg tatcgaagcc ttaaacctct gtctgcgagc
     2341 ggttgccaga gccggtgaca cgattgcaat tgaatctccg acctattatg gcgtcttgca
     2401 ggtgattgaa aatcttggca tgcgtgcact tgaaattcca accagcccaa cggatgggat
     2461 ttgcctggat gcgcttgaaa ccgcccttca ttcgcaatcc attgcggcct gtctggtgat
     2521 gtccaacttc aataatccac tgggaagctg tatgccagat gccaaaaaac aacggctggt
     2581 tgagatcctg gctggccacg ttgtgccttt gattgaagac gatatttacg gcgatttgaa
     2641 ctttggcacc acacgtccca aaacgtgcaa ggcatttgac cgtgatggac tggtgttgct
     2701 ctgtgcgtcg ttttccaaga ccctggcccc aggatatcga atcggctgga ccgcaccagg
     2761 gaggtttcgg gctcaggtcg agcggctcaa aggaatgaac accattgcca ccgcgacgct
     2821 cccacaaatg gtagtggctg aatttttgaa aaatggcggc tatgaacgct atttgcggca
     2881 gttacggctg cattttgcct cgcatctgta tgccgccaag caggcaatct tccagtcttt
     2941 tccacccgga accaaggtga cacgtccagg cggagggttt gtcctgtggg tggaatttcc
     3001 agaaggcatt gatacccttg aactgcacta tcgtgccctg gaacacaaaa tcagcctcgc
     3061 gccaggggtg atgttttcac catcaaaaaa ataccagaat tgtgttcggc tcagttttgg
     3121 tcagaaatgg tcagaaacaa cccagcgggc gattgaaacc gtcggcaaac tggccagcca
     3181 gggcacataa gtaccttgtc ataagtttcc tgagatattg ggtttggtac gtggttgatt
     3241 tttttccgtg ttttccgtgg tgaaaatgtc tggaaatttt cggtaaagta ctgaacggca
     3301 ccagggccag gcaagatact tgaaccagcc acgagcaact gtgtctccgc gcaatcaccg
     3361 gagcgtcagg gtgtcatccg tgaaagattc ctgtattgtt tctgccacag aaacaatcat
     3421 cccttcaaaa agattcatat tctgcccctt gtgctc
//
LOCUS       DAOQDO010000393         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20457_length_3456_cov_2.540429, whole genome shotgun sequence.
ACCESSION   DAOQDO010000393 DAOQDO010000000
VERSION     DAOQDO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20457_length_3456_cov_2.540429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKZ53_07600"
     CDS             complement(<1..529)
                     /locus_tag="PKZ53_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ40337.1"
                     /translation="MKIKPPEYNDPLLSRTEDEAFRGSQLLRSFLRNLPLPSPQEIFT
                     KLEQLGYHGQVAQRRAVSLMAYRHVKRLKRLHVDGEPLRKLPPKQNVLMIGPTGCGKT
                     FLVELLFQHIFRLPTVIVDITSFTESGYVGDDVRTILTRLISASGDDPELASCGVICL
                     DEFDKLSASGSNARFA"
     gene            complement(510..641)
                     /locus_tag="PKZ53_07605"
     CDS             complement(510..641)
                     /locus_tag="PKZ53_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40338.1"
                     /translation="MKPEPALPSPSSLKNPVWYQSQVVLVEFSVISESGQIYENQAP"
     gene            952..1533
                     /locus_tag="PKZ53_07610"
     CDS             952..1533
                     /locus_tag="PKZ53_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNJ40339.1"
                     /translation="MYCPQCRQVIPDGSRFCNLCGAVLGAPSQAGTLAPHGAPLPAEV
                     VHSVMHPSFIFVGVRYTVAAILSVVIVILGAMIAGKAWEWLSNLIPWVVGFGVVVLFL
                     NPIYNHILRQIETYTLTSEKVEIQTGLLVKRTQNIPLQKIQDVSTQITLLNRMLGIGD
                     VVIDSASSSGKIILRHVTNPQAVASEILARVRK"
     gene            1578..2870
                     /locus_tag="PKZ53_07615"
     CDS             1578..2870
                     /locus_tag="PKZ53_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40340.1"
                     /translation="MVTRLISKSGLVILGILALGGIAQSGWAQNSNPAAIPLAIMPKK
                     EPPTAVEATGTQFPIQEFLNSRLGKATEVMTVNGDHIQEFSPDIAPLLTESSVQQGYQ
                     GKVGNYTVQVFQFPHPDYAFGVFSTLRPAKDKALVNKESVWVVGQMAGAWVQSEFVMV
                     RTLTGDASATQLEQLATEVITKLQSWHARQPGYDPKAPKTPFVVRHLPAAHLDPETLR
                     FAVGQIGISTNAPEPGFQEFPVKDGMGFTWASYGPESPFQRLVIADYPTPQDATSALY
                     KIAIHANRLPTEERTRRIVQRRGNFIVEAYGVADWMKTQAVISTIEYDYEVQWLTGGL
                     DQPPPPPEPPGLDPVVEAHKTATMLISIFRLIGLGASVSVVVGLAVGLGFFYWRRKST
                     MSDSIFSDAGGMLRLNLNDSFSIPASVESGLKQLEPGE"
     gene            2875..>3456
                     /locus_tag="PKZ53_07620"
     CDS             2875..>3456
                     /locus_tag="PKZ53_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ40341.1"
                     /translation="MAVSEPTPSTVYDLLIIGAGPAGLAAGYRAEQAGLSYLILEKGI
                     IAQTVYNYPIGKPLFSTPNEVELAPGTLKPKNEKPTREEVLAYYNQFALREHRMRIHL
                     HESVHQILREADGRFLIVTPKAMYRAHRVLVATGGFGLPRKLGVPGETESRVSYRFVE
                     AFAYGGQDVLVVGGGNSAAEAVLFLHEGGANVSW"
BASE COUNT          823 a          850 c          931 g          852 t
ORIGIN      
        1 cagcaaaccg ggcattgctg cctgatgccg acaacttgtc aaattcatcc agacaaatca
       61 caccacacga cgccagttcc gggtcgtcgc ccgaagcgct gatcagtcga gtgaggatcg
      121 tccggacatc atcgccgaca taaccgcttt cggtaaaact ggtgatgtcc acaatcacgg
      181 tcggcagccg gaaaatatgt tgaaagagca attcaaccag aaacgttttt ccacatccgg
      241 tcggaccaat catcagtaca ttttgcttgg gcggtaattt gcgcaggggt tcgccatcaa
      301 cgtgcaggcg tttgagtcgt ttgacgtggc ggtaggccat taaagagacc gcccggcgct
      361 gcgccacctg accgtgatag cctaattgtt ccagtttggt gaagatctcc tgcggtgagg
      421 gcagtggcag gttgcgcaaa aacgaacgca gcaattgcga gcctcggaac gcttcatcct
      481 ctgttcggga gagcagcggg tcgttgtatt cagggggctt gattttcata aatttgaccg
      541 gattcggaaa tcactgaaaa ctcaaccagc acaacctggg actgatacca tactgggttt
      601 ttgagcgatg atggcgatgg gagcgccggt tctggtttca tagcgctaac ttgatgagaa
      661 atcaactggt tcacaacatt catgtcagcg ctggtctatc cagggtagtc gaaaaacgcc
      721 caatctcaaa tctggatgcg aaacttttac gacttttgga acttcctgcc ctgagtggtg
      781 tctaatcggg gaattgaaaa aagctcaagc cacaaccagt cggtcgtcat ttgtaacatc
      841 catcagatca agtacttgac tctttgaaag ggaagctcta atcgttcatt ccccaaaaac
      901 tgtggtaaga tgtcgccatc cagtcatctt cttcaatcag cgagtctctc catgtactgt
      961 cctcaatgtc gtcaggtgat tcctgacggg agccggtttt gtaatttgtg tggtgccgtg
     1021 ctgggtgccc catcgcaagc gggcacgctg gcgccgcacg gtgcgccatt gccagccgaa
     1081 gtggttcatt cggtgatgca tcccagtttt atttttgttg gggtgcgata tacggtggcg
     1141 gccattctat cggtggtgat tgtgattctg ggggcgatga ttgccgggaa agcctgggag
     1201 tggctgagta atttgattcc ctgggtggtt ggatttgggg tcgtggtgct gtttctcaat
     1261 ccgatttaca accacatttt acggcaaatc gaaacttata cgttaacgtc tgagaaagtc
     1321 gaaattcaaa ccggacttct ggtgaaacgg acccaaaaca tcccgctcca aaaaatacag
     1381 gatgtttcaa ctcaaatcac attgttaaac cgaatgttgg gtattggtga tgtggtgatt
     1441 gatagtgcgt cctcatccgg aaaaattatt ctccgtcacg tgaccaatcc gcaggcggta
     1501 gccagcgaga tcctggccag agtgcggaaa taagcggtcc aaagcaacac tttgaagcaa
     1561 agagtggagg caattcaatg gtgactcggt tgatttcaaa atccgggctg gtgatcctgg
     1621 gaattctggc tctggggggg attgcccaat ccggatgggc acaaaattca aacccggcgg
     1681 cgattccact ggccatcatg ccgaaaaaag agcctcccac cgcggttgag gcaacgggga
     1741 ctcagtttcc gattcaggaa tttctgaact cccgccttgg aaaagccact gaggtcatga
     1801 ccgtcaacgg cgaccacatt caggaatttt cacctgacat cgccccgttg ttgaccgaat
     1861 caagcgttca gcaagggtat caggggaagg ttggcaacta cacagttcag gtatttcagt
     1921 ttccgcatcc agattatgcg tttggggtgt tttccacgct ccgcccggcc aaagacaaag
     1981 ccctggtgaa taaagaatcg gtgtgggtgg ttgggcagat ggcgggtgcc tgggtccaat
     2041 cggaatttgt gatggttcgg actttgaccg gggacgcatc cgccactcaa ctcgaacaac
     2101 tggcgaccga agtcatcacc aaacttcaat cctggcatgc cagacaacca ggatatgatc
     2161 ccaaagcacc caaaacgccg tttgtggtac gacacctgcc agccgcccac cttgatcccg
     2221 aaaccctgcg ctttgccgtt ggtcagattg ggatatccac caatgcgcca gagcccggtt
     2281 ttcaggaatt cccagtcaag gatggcatgg gctttacctg ggccagttac gggccagaaa
     2341 gtccttttca acggttggtg attgctgact atccaacacc gcaggatgcc acgtcggcac
     2401 tctacaaaat tgccatccat gccaaccggc tgcccactga agaacgaacc cggcgcatcg
     2461 tccaacgtcg gggtaatttt attgttgaag cctatggtgt ggctgattgg atgaaaacgc
     2521 aggccgtcat ttcgaccatc gaatatgatt atgaggtcca gtggttgact ggtgggcttg
     2581 atcagccgcc accaccacct gaaccgcccg gtctcgatcc cgtggttgaa gcgcataaga
     2641 cagccacgat gttgatcagt atttttcgtc tgattggatt gggggcgtca gtttcggttg
     2701 tggttggact ggccgttggt ttagggtttt tctactggcg tcgaaaatca accatgtcag
     2761 attccatctt tagtgatgcc ggcggtatgt tgcgattaaa tttgaacgat tcgttttcga
     2821 tcccagcgtc agtggaatct gggctaaaac aattggaacc tggagagtag tgttgtggct
     2881 gtatctgaac ccacaccatc aacggtgtat gatctgttaa tcatcggcgc tggacctgct
     2941 ggactggccg ccggatatcg agctgagcaa gccgggctgt cctatctcat actggaaaaa
     3001 ggcattattg cccaaaccgt gtataactat cccattggaa aaccattgtt ttcaacacca
     3061 aacgaagttg aactggcgcc aggtacgctc aaaccaaaaa atgaaaagcc gacccgtgaa
     3121 gaagttctgg cctattacaa ccagtttgcc ctgcgtgaac accggatgcg gattcacctg
     3181 cacgagtcag ttcatcagat tttacgtgaa gctgacgggc gctttttgat tgtgacacca
     3241 aaagcaatgt accgtgccca tcgggttttg gtggcgactg gcggatttgg actgccgcgc
     3301 aaattaggcg ttcctggcga gaccgaaagc cgtgtttcct atcggtttgt tgaagcattt
     3361 gcctacggtg gtcaggacgt attggtggtt ggcgggggaa attcagccgc tgaggcggtt
     3421 ctctttttgc acgaaggcgg tgccaacgta tcgtgg
//
LOCUS       DAOQDO010000394         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20478_length_3453_cov_3.184520, whole genome shotgun sequence.
ACCESSION   DAOQDO010000394 DAOQDO010000000
VERSION     DAOQDO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20478_length_3453_cov_3.184520"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKZ53_07625"
     CDS             complement(<1..889)
                     /locus_tag="PKZ53_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40342.1"
                     /translation="MQMKMVWLVVVGFVLTGCGVWMQLAPVASASHSKSRVRQNVTSP
                     DGVWQTIEPGQARSGFSMRLASTAQTHQCTLNRTVFARLMASAPMEFSAQARQRQTIM
                     TFPMPDGSFQRFRIEESPIFAPELAAQFPEFKTYHGQGIDDPTAMVRFDWTSKGFHAM
                     VWSAGDRVVIDPEGDGNTDVCVSYSSRDLRRIAESPQCEAVERDTAAIFSNQGTELKT
                     VEPRQSGSLTVGRQLRTFRLALGATGEYTRFHGGTVEAGLAAMTTTVNRINGIYSREL
                     AIRLMLIPQETAIIYTDPAT"
     gene            complement(910..1794)
                     /locus_tag="PKZ53_07630"
     CDS             complement(910..1794)
                     /locus_tag="PKZ53_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ40343.1"
                     /translation="MTESVVTLNIEDIITEDDTPVDNLPSEKQQRLLTEPLYSSWQRP
                     GGGYFLAAANVGIFNSVHLPPIVPDMFLSLDVQVADDWWQKEHRSYFTWEFGKSPEVV
                     IEIVSNRVGEEAGLKFTRYATIGVVFYAIFDPMKFLKGDVLAVYTLRSGRYEKMESCE
                     LSPIGLKLDLWDGEFEGKQNSWLRWFDLEGNVIPTGKERAEAERQRAEAECQRAEAEH
                     QRAEAERQRAEAERQRAEAEHQRAEAECQRAEVEHSEKEQALQLVEQERSEKEAALLR
                     AEQLAARLRELGIDPDNL"
     gene            2030..2839
                     /locus_tag="PKZ53_07635"
     CDS             2030..2839
                     /locus_tag="PKZ53_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNJ40344.1"
                     /translation="MQTETSIEKYQIAKEPFYLPVGNEIELFRAAYQSKLPVMLKGPT
                     GCGKTRFVEHMAWRLSRPLVTVACHEDLSATDLVGRYLLEGDQTVWHDGPLTTAVRHG
                     AVCYLDEVVEARKDTLVLIHPLSDDRRILPIEKRGVILSAPDNFLLVISYNPGYQSVL
                     KDLKQSTRQRFISIEFDYPPAKIEAEIVARESGVDQETAQDLVKIGEKVRNLKGHGLE
                     EGVSTRLLVYAGQLIKQGISPVTACEVSIVNPITDDSQLQRSIREIVTTVL"
     gene            2931..3236
                     /locus_tag="PKZ53_07640"
     CDS             2931..3236
                     /locus_tag="PKZ53_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNJ40345.1"
                     /translation="MPQFTTFQEPRIHFFPEEILQKLCEQYPGISVDKEIFGGVPHLK
                     RVRLTVAQILSQLYVRGSIEAIVSYYSGEVSAEQVKEAIAFAHDFMELTCDPPQIDG"
     gene            3211..>3453
                     /locus_tag="PKZ53_07645"
     CDS             3211..>3453
                     /locus_tag="PKZ53_07645"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNJ40346.1"
                     /translation="MTRLKLMVDYNLGKPVADRVNTLGKKVEATTTIDHGFRSNTADA
                     DLVAYARKAGILLLTHDVNTMDERTFPPCSHGGIIIV"
BASE COUNT          869 a          887 c          837 g          860 t
ORIGIN      
        1 ccgtggccgg atcagtgtag ataatggccg tttcctgggg aatcaacatc aggcggatgg
       61 ccagttcccg gctgtaaatc ccattgatgc gattgaccgt ggtggtcatc gccgccagtc
      121 cggcttcgac cgttccgcca tgaaaccggg tgtattcacc ggttgctccc agcgccagcc
      181 ggaaggttcg aagctgccgt ccaactgtca ggcttcctga ttgtcgcggt tcaaccgttt
      241 tgagttccgt accctgattt gaaaatattg cggcagtgtc tctttcgacg gcttcgcact
      301 gtggagactc tgcaatccgt cgcagatcac gtgaggagta gctcacgcag acgtccgtgt
      361 ttccgtcccc ttccggatca atcacaactc gatctccagc cgaccagacc atggcgtgaa
      421 atccctttga cgtccaatca aaacgaacca tcgcggttgg gtcatcaatc ccctgcccgt
      481 gataggtttt aaattctggg aactgggccg caagttccgg ggcaaaaatc ggggattctt
      541 caatccgaaa ccgttgaaaa cttccgtccg gcatcggaaa cgtcataatt gtctgccgtt
      601 gccgcgcctg tgctgaaaat tccatcggag cggaagccat caatctggca aacaccgtcc
      661 gattcaaagt gcactgatgc gtttgagccg ttgaagccag acgcattgaa aacccagagc
      721 gagcttgccc aggttcaatc gtttgccaca ctccatcagg tgaagtcacg ttttgtcgaa
      781 ccctggattt tgaatgactg gcactggcaa ccggggcaag ttgcatccaa acaccacaac
      841 cagtcaaaac gaatccaacc acaaccaacc agaccatttt catttgcatt ccgctctctc
      901 cttgtacgtt caaaggttat cgggatctat gccaagttcg cgtagccggg cggccagttg
      961 ctcagctcgg agtaaggccg cttctttctc gcttcgctct tgttcgacga gttgtaaagc
     1021 ctgttctttt tcagagtgct cgacttcggc tcgttgacat tcagcttcag ctcgttgatg
     1081 ttcagcttca gctcgttgac gttcagcttc agctcgttga cgttcagctt cagctcgttg
     1141 atgttcagct tcagctcgtt gacattcagc ttcagctcgt tgacgttcag cttcagctcg
     1201 ctctttccct gtcggaatga catttccttc cagatcaaac cagcgcagcc acgaattttg
     1261 cttgccttca aattcaccgt cccataaatc cagtttcagt ccaatcggtg acagttcaca
     1321 cgattccatc ttttcgtagc ggccactccg gagggtatac acagccaaaa catcgccctt
     1381 gagaaatttc atcggatcaa aaatggcata gaaaaccaca ccgatggtgg catagcgggt
     1441 aaacttgagg cccgcttctt caccaactcg atttgagacg atttcaatta ccacttcagg
     1501 agatttgcca aattcccagg tgaaataaga ccgatgttct ttttgccacc agtcatccgc
     1561 aacctgaaca tccaggctta aaaacatgtc tggaacaatc ggaggcaggt gaactgagtt
     1621 aaaaatgcca acattggctg cggccaggaa gtatccacca cctgggcgct gccacgaact
     1681 gtagagtggc tcggtgagca ggcgctgttg tttttcagaa ggcagattat ccacaggcgt
     1741 gtcatcctcg gtaatgatat cttcaatgtt tagtgtaact actgattcag tcatacaacc
     1801 tctatcggtg aaagacgctt ttgggttcgc gacaatgcag cgtcaggtca acagggtttg
     1861 gaagtcaaat gggttgagat tggttgagct ggcagtatag cactggctgc cacttgaaag
     1921 ccaaatcaca ccaaaccaaa gagatactca aggtcgccaa aaagtttgag aaagaagccg
     1981 ggagttggtg gcttgtcaat tttccagctt cattggtaaa gtgcgaaata tgcagacaga
     2041 aacttcaatc gaaaaatacc aaattgcgaa agaaccgttc taccttcccg ttgggaacga
     2101 aatcgaactc tttcgtgcgg cctatcagtc aaagcttccg gtgatgttaa agggtccaac
     2161 cggatgcggg aaaacccggt tcgtcgagca tatggcatgg cgattgagcc gcccgctggt
     2221 gacggtggcc tgccatgaag atttatcggc caccgatctg gttggccgct atctgctcga
     2281 aggcgaccag accgtctggc atgacggccc gctgacgaca gccgtcaggc acggggcggt
     2341 ttgctatctg gacgaagtcg tcgaagcccg caaagacacg ctggttctga tccacccctt
     2401 gagcgatgac cgacgcatcc tgccgattga aaaacgcggc gtgattttga gtgccccaga
     2461 taattttctg ctcgtgattt cctacaaccc cggctatcag agcgtgttaa aagacctcaa
     2521 gcaatcaacc cgccagcgct ttatcagcat tgaatttgat tatccgccag ccaaaatcga
     2581 agccgaaatc gtagccaggg aaagcggtgt tgatcaggaa accgcccagg atctggtcaa
     2641 aatcggtgaa aaagtgcgca acctgaaagg ccacggtctg gaagaaggcg tctcaacccg
     2701 actgctggtc tatgccgggc aattgatcaa acaaggtatt tccccggtca ccgcctgtga
     2761 agtctctatc gtcaacccga ttaccgacga tagccagctc cagcgcagca ttcgggaaat
     2821 tgtgacgacg gttttgtaac ttcaagcaaa ccaccaacaa agctttacgc ccaaacctgg
     2881 taagagtaag gtttttgtga gttatccctt ttatcagtgg aggtagatca atgccacaat
     2941 ttacaacttt ccaggagcct cgaattcatt tttttcctga agaaattctt caaaagttat
     3001 gtgaacagta tcctggaatt agcgttgata aagaaatttt tggcggggta ccacatttaa
     3061 agagggttcg ccttaccgtt gctcaaatac ttagccagtt gtatgtgcga ggaagtattg
     3121 aggcaattgt aagttattat tctggagaag ttagtgctga acaagttaaa gaagcaatcg
     3181 cctttgccca cgattttatg gagttgacct gtgacccgcc tcaaattgat ggttgattac
     3241 aaccttggga aacccgtggc cgacagggtg aatactttag ggaaaaaagt ggaagccaca
     3301 acaaccattg accatgggtt caggtcaaac accgctgatg cggacctggt tgcttatgca
     3361 cgaaaggcag gaattcttct tcttactcac gatgtaaata caatggatga gcgtacattc
     3421 cctccttgct cacacggggg tattattatt gta
//
LOCUS       DAOQDO010000395         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20485_length_3452_cov_4.831911, whole genome shotgun sequence.
ACCESSION   DAOQDO010000395 DAOQDO010000000
VERSION     DAOQDO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20485_length_3452_cov_4.831911"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1476)
                     /locus_tag="PKZ53_07650"
     CDS             complement(196..1476)
                     /locus_tag="PKZ53_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNJ40347.1"
                     /translation="MMTRRNFIQTAGLGVAAIGTSSLMPNILTGFSGSVSADAKTKTS
                     SKVLVCLFQRGAADGLNIVVPFTERSYYDLRPTLAIPEPKSGNPKAAINLDGKFGFHP
                     TLAPLKPYFDKGQLAVIHAAGSPDATRSHFDAQDYMESGTPGRKSTTDGWLNRYLAAS
                     VEPGDTPFRGVALTQLTPRSMQGAADVVAMANIGSFNLRAGSNSSVKSSFEEMYAQSA
                     TDALRGTGHDTFDAVNFLRKANPEQFKVENGADYPRGELGNSLRQIAQLIKADVGLEV
                     AFAEMGGWDTHNNQGDPGNGQGVGRLSRLLRDFGGSLAAFAQDLGPRMQDVVVLTMTE
                     FGRTARQNGTGGTDHGHASCMFVLGGPVKGGKVYGQWPGLGANDLYEGRDLAVTTDFR
                     DVVAEVLTRHMGAHNLRSVLPGYTVNEKNFKGLL"
     assembly_gap    1544..1643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1722..3377)
                     /locus_tag="PKZ53_07655"
     CDS             complement(1722..3377)
                     /locus_tag="PKZ53_07655"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNJ40348.1"
                     /translation="MTQTELMEIEKESREFRNQIVKQGIKRDENGKIDPESISPEARE
                     MLQNRMESGEMNAREIPRELQEAKLLRAVYSERQLQEVLTDFWMNHFNIFIGKGLDRV
                     FLTSYERDTIRPHIFGKFEDLLTATAESPAMMFYLDNWQSVDPNMKLPDMSQLGKRQN
                     KGAFGKGRNRRLFQNGGNRQAEMPDPEKLQALQNQLKNRKFGLNENYARELLELHTLG
                     VDGGYTQKDVTEVARCFTGWSINRPLNGNRMKKMGLGGGEPGTFIYRDLTHDKGEKSV
                     LGTTIAAGGGKSDGETVLHMLATHPSTAKFISTKLARRLIADQPPSRVVDAAAATFKR
                     TGGDLREVVKTIISAPEFFSAEAYRAKVKTPLELVASSLRATDADIQRTTPMIMVLRQ
                     LGMPLYGWQPPTGYPDTADHWVNTGALLNRLNFSLALAGNRIPGVKVNLNELTSGART
                     PEAELNTLIKYFLSNDISAQTRNSILEGLNQVNTPSSDRAEPSDPDEPPMMRGKNKGE
                     KPFGKGRGKGQLELASLVKNGGPLSETAQLTGLILGSPEFQRK"
BASE COUNT          715 a          899 c          854 g          884 t
ORIGIN      
        1 cttggttctt ggtgcttggt tcttggttct tggttcttgg tgcttggttc ttggtgcttg
       61 gttcttggtt cttggttctt ggttcttggt tcttggttct tggttcttgg ttcttcgaag
      121 ctatttggtt ccaaagcacc aagcaccaag cactaaacac caatcactaa gcaccgtcac
      181 caattttcat cttttttata aaaggccctt gaaatttttt tcattcaccg tgtatcccgg
      241 cagcactgac cgaagattgt gtgcgcccat gtgacgggtg agaacctcgg caacgacatc
      301 gcgaaaatca gtcgtgactg ccagatcgcg gccttcatat aagtcattgg cgcccaatcc
      361 aggccactgc ccatagactt tgccaccttt gactgggcca cccagcacaa acatacacga
      421 agcatgtccg tgatcggtcc cgccggtgcc attttggcgt gccgtgcggc caaactcagt
      481 catggtcagg accaccacgt cctgcattcg agggccaaga tcctgagcga acgccgccaa
      541 cgagcctccg aaatcccgga gcagacgtga caaccgaccg acaccctgac cattgccggg
      601 gtctccctgg ttattatggg tatcccagcc acccatttca gcaaatgcca cctcaaggcc
      661 aacatcggct ttgatgagtt gggcaatctg gcgcaggcta ttgccgagtt caccacgtgg
      721 gtagtcggca ccattttcaa ctttgaattg ctcaggattg gctttgcgca aaaagttcac
      781 cgcatcaaat gtgtcgtgtc cagttccgcg cagggcatca gtggctgact gagcatacat
      841 ttcctcaaag ctggacttga cggacgagtt gctcccggcg cgcaggttaa agcttccaat
      901 gttggccatc gccacgacat cggcagcccc ctgcatcgag cgcggtgtca actgggtcag
      961 ggcaacacct cgaaacggcg tgtctcccgg ttcaaccgat gccgccagat agcggtttaa
     1021 ccagccatca gtggtgcttt ttcgccccgg agttcctgat tccatatagt cctgggcatc
     1081 aaaatgagaa cgggtcgcgt cgggtgatcc agccgcatgg atcacagcca actgtccctt
     1141 gtcaaaatag ggttttaacg gtgccaacgt cggatgaaag ccaaatttgc catccaggtt
     1201 gatcgccgct ttggggttgc cgctctttgg ctctggaatc gcaagcgttg gccgcaggtc
     1261 atagtagctt ctctcagtaa acggcaccac gatattcaat ccatctgctg cgccacgttg
     1321 gaagaggcac accagcactt ttgatgatgt tttggttttt gcatcggctg aaaccgagcc
     1381 ggaaaatcca gtcaagatgt tgggcatcaa cgatgatgtt ccaatcgcag ccaccccaag
     1441 cccagccgtt tgaataaagt ttcgacgtgt catcatggca atttctcctt ccgggttccg
     1501 ggttccgggt tccgggtttt cgataagtcc tgctcactat ttcnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnttccggg ttccgggttc cgggttccgg gttttcgata
     1681 agtcctgctc actatttcgc tacttcgcta cttgccacag ctcacttacg ttgaaattcc
     1741 ggcgaaccaa gaatcaaccc ggtcagttgg gcagtctcag aaagcggtcc accatttttc
     1801 acgagcgaag ccagttccag ttgtcctttg ccgcggcctt ttccaaaagg cttctcgcct
     1861 ttgtttttgc cacgcatcat tggtggttca tccgggtcgg atggttcagc cctatcagag
     1921 gaaggagtat tgacctgatt gagaccttca aggatcgaat tgcgcgtttg ggcggaaata
     1981 tcgtttgaaa ggaagtactt aatcagcgtg ttgagttcag cttccggggt gcgggcgccg
     2041 ctggtgagtt cgttaaggtt gactttgacc cctggaatgc ggttgccagc cagtgcgagg
     2101 ctgaaattga gacggttgag caaggcaccg gtgttaaccc aatgatcagc cgtgtctgga
     2161 tatccggttg gtggctgcca gccatagagc ggcattccta actgtcgcaa aaccatgatc
     2221 atcggggttg ttcgttgaat gtcagcatcg gtcgcccgca atgaactggc caccagttcc
     2281 agtggcgttt tgactttggc gcgataggct tcggcagaga aaaattccgg agcagaaatg
     2341 atcgttttca cgacttcccg caaatcacca cccgtgcgct taaaggtcgc agccgcagca
     2401 tcaacaacac gggatggagg ctggtcggca atcagccggc gagccagttt ggtcgagata
     2461 aatttcgcag ttgatggatg tgtggccagc atatggagca cggtttcacc gtcgcttttc
     2521 ccgccaccag cagcaattgt ggtcccaagc actgactttt cacctttatc gtgagtcaga
     2581 tcacgataaa taaaggtccc gggctctccg ccaccaaggc ccatcttttt catgcggttg
     2641 ccattcaacg gacgattgat gctccaacca gtaaaacatc gggcgacttc ggtgacatcc
     2701 ttttgggtat atccgccgtc aaccccaagc gtgtggagtt caagcagttc gcgggcatag
     2761 ttttcattga gcccaaactt tcgatttttc aactggttct ggagcgcctg gagtttttct
     2821 ggatccggca tctcagcctg acgattgcca ccattttgga acagccggcg gttccttcct
     2881 tttccaaaag cacctttgtt ctgacgcttt ccaagctggc tcatatcagg cagcttcatg
     2941 tttgggtcaa ccgattgcca gttatcaaga taaaacatca tggccgggct ttcagccgtc
     3001 gccgtcagca ggtcttcaaa ttttccgaaa atatgaggcc ggatggtgtc gcgttcataa
     3061 ctggtcagaa agactcgatc caacccttta ccaataaaga tgttaaagtg attcatccag
     3121 aaatcggtca ggacttcctg gagctgacgt tcgctataca ccgcccgcaa gagcttggct
     3181 tcctggagtt cgcggggaat ttcacgggca ttcatttcgc cagattccat tcgattctgg
     3241 agcatttccc gagcttcagg agaaattgat tctggatcaa ttttgccgtt ttcatcgcgc
     3301 ttgattccct gtttgacaat ctggttgcga aattcacggc tttccttctc gatttccatc
     3361 agttcggttt gggtcaacgt gagggtttta aactccgcgc gaaaccatca aggccttgcg
     3421 caagaacgtt cttgcgaagt gctatggtgg cg
//
LOCUS       DAOQDO010000396         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20490_length_3452_cov_3.311451, whole genome shotgun sequence.
ACCESSION   DAOQDO010000396 DAOQDO010000000
VERSION     DAOQDO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_3452_cov_3.311451"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2984)
                     /locus_tag="PKZ53_07660"
     CDS             complement(<1..2984)
                     /locus_tag="PKZ53_07660"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40349.1"
                     /translation="MRDCFLLFALATWFGLTPVSGVAASKRQAPLLDLNHPLEKGLKS
                     GEKHEFRLTVEANTLVKVLVEQISLDVVVTLQTADGQLVAETNSTPANFEAEQLFLVL
                     ERAGTYLVQVTPVAKEPSEGKFTITLAEVHPATAQDLQRVALEKQYQRGLELAADPTK
                     EKQAQAIETFQAVAMQAEISGDTALQIEAFNQVGTRLFGQGKPKLALPAFEKVLELVR
                     TLKDAKKEASTLNNLGVVHNLLGNSDKAIEYLEQTLPLQKATGNLKGYGMTLNNLGAI
                     YTKLGQKQKTLDYLEQALVVHRQVNNQAGAAQTLANLGKLYVEVGNRSKALEVTQEAL
                     SIAQAIGNPAQEISALGVLGDSYNQWGETKKALPYFEQALAICQRIGDQARETAMLSN
                     LAAIYKSLGDFQKALNTYDRVLALTRAVGNRSWEASTLANQGSVYQKLGQNSKAIQLT
                     EEAYGLAQKVDPTMVPVYLLTLGVLYGNDQNWAKSHELFEQVLGQFQQQKNARWEAVA
                     LSNLGWVESQTGNYPKAIEYLNQANAVRQTIGNRYESAGTFHNLGWAYSQMKDYPQAE
                     ANYKKALDLDQELGHKSGIARTLGHLALVEASQKKLIQARKSIEQAIEILESIRSDVT
                     NQELRATYFSSNSRYYKTYIDILMALHQQSPEAGYAAHALTVSERARARSLLELLTEA
                     NLDLKEGIAPELLDREQELRHKIEFKLGKLSQLLAQKHTVEQETTAKAEIEQLTDEFR
                     QVQAEIREKSPRYGALTQPQPMTASEIQRRVLDSDTVLLEYFLGSETSFLWALSHQTL
                     NVYALPGQAVIDELVQKSHAGWKTRRQRAIARKTGDINSPAGISQDPARQLSQMILGP
                     AAKELAGKRLVVVPDGSLHYVSFAALPDPTSSVGEPLVVHHELVVLPSASTLGVLREE
                     AERRKPAEKTLAVLADPVFSLEDTRLLNHKSASAPDLHSEPTALSETSRAIEARLIKG
                     GDTSPLQGEGTRAQRSLVLARLP"
     gene            3100..>3452
                     /locus_tag="PKZ53_07665"
     CDS             3100..>3452
                     /locus_tag="PKZ53_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40350.1"
                     /translation="MTKHSIGVKNRCVNPFVICPASLGETSAIFRPYPLAKSKKPTEH
                     SMFKPRFGYLAVVAIALQFCGNSLFAQSVPGEIPGLQKTRKPANKVNLGPTTGLGADK
                     QGRIYIVSNTRNRVYR"
BASE COUNT          762 a          907 c          894 g          889 t
ORIGIN      
        1 ggcaatctgg ccaggaccag actccgctgg gcgcgggttc cttctccctg aagagggctg
       61 gtgtctccgc ctttgatcaa ccgcgcttca atcgcccgac tggtttccga caaagctgtc
      121 ggttcggaat gtaagtctgg ggcagacgcg gacttgtggt ttaaaagccg ggtatcttcc
      181 agactaaaca cgggatcggc cagaaccgcc agtgtttttt cggctggttt tcgccgttcg
      241 gcttcttcgc gcaacacccc cagggttgaa gccgatggca gcaccaccag ctcgtgatgc
      301 acgaccagcg gttctcccac tgaactggtt ggatcaggta aggcggcaaa cgatacatag
      361 tgcagcgatc cgtcaggcac aaccaccagt cgtttcccag ccagttcctt cgctgccggg
      421 cctaaaatca tctggcttaa ttgtcgggct ggatcttgtg aaattccggc aggtgaattg
      481 atgtctccgg ttttacgggc gattgcgcgc tggcggcggg ttttccatcc cgcgtgggat
      541 ttttgcacga gttcgtcaat aacggcttgc cccggcaagg catacacatt gagtgtttgg
      601 tgagagagtg cccacaaaaa gctggtttcc gatcccaaaa aatattcgag cagcaccgtg
      661 tcagaatcca aaacgcggcg ctgaatctcg ctcgccgtca ttggctgtgg ctgagtcaac
      721 gcaccgtagc gagggctttt ttcgcggatt tcggcctgaa cctgccgaaa ttcatcggtc
      781 agttgctcaa tctcagcttt ggcggtggtt tcttgctcaa cggtgtgctt ttgggcaagc
      841 aactgagaca acttcccaag cttgaactcg attttgtggc gaagttcctg ttcgcggtca
      901 agcaattccg gtgcaatgcc ttccttgaga tcaaggttgg cttcggtgag caattccagc
      961 agcgagcggg cccgggcccg ttcggaaacg gtcagggcgt gagccgcata cccggcctcg
     1021 ggtgattgct gatggagcgc catcaaaata tcaatatatg ttttgtaata ccggctattt
     1081 gaagaaaaat acgtcgcacg taattcctgg tttgtaacgt cgctgcgaat ggattccagg
     1141 atttcgatgg cctgttcaat ggattttcgg gcctggatca gtttcttttg actggcttca
     1201 accagagcca ggtggcctaa ggtccgggca ataccgcttt tgtgaccaag ttcctgatcc
     1261 aggtcaagtg cctttttgta attggcttcg gcctgcggat aatccttcat ctgggaatag
     1321 gcccacccaa gattgtgaaa agtcccagcc gattcatagc gattcccaat ggtttggcgt
     1381 accgcatttg cctggttcaa atattcaatt gcttttggat aatttccggt ttgactctcg
     1441 acccagccca ggttgctcag agcaaccgcc tcccaccggg catttttttg ttgctgaaac
     1501 tgccccagca cctgctcaaa caattcatgg gatttcgccc agttctgatc attgccatac
     1561 aacacgccca gcgtgagtaa atacaccggc accatggtcg ggtctacctt ttgagccagc
     1621 ccataggctt cttcagtcaa ttgaatagct tttgagtttt ggcccaactt ttgatacacc
     1681 gagccttgat tggccagggt actggcttcc caactccggt tgccgacggc acgggtcagg
     1741 gcaagcaccc gatcataggt gtttaacgct ttctggaaat caccaaggga tttatagatg
     1801 gctgccaggt tgctgagcat tgccgtctcg cgggcctggt cgccaatccg ctggcaaatc
     1861 gccagtgcct gttcaaaata gggcagggct tttttggttt caccccattg attgtaacta
     1921 tctccgagga cgcccagggc agaaatttcc tgtgcggggt tcccaatggc ctgggcaata
     1981 gacaacgctt cctgggtaac ttccagagct ttggaacggt ttccaacctc aacatagagc
     2041 ttgcccaaat tggccagcgt ttgggccgca cctgcctggt tgttgacctg tcggtggacc
     2101 accagtgctt gctcaagata gtcgagcgtt ttttgttttt gccccagctt ggtatagatg
     2161 gcgccaagat tattgagcgt catgccatac cctttgaggt ttccagtggc tttctgcaaa
     2221 ggcaacgtct gttccagata ttcaatggct ttatcagaat tacccagcaa attgtggaca
     2281 acgcccaaat tgttgagggt tgaggcttct ttttttgcat ctttcagcgt tcggacgagt
     2341 tcaaggactt tctcaaacgc tggcagcgcc agtttgggtt ttccttgacc aaacaagcgg
     2401 gtgccgactt gattgaaggc ttcgatctgc aaggcggtat caccacttat ttcggcctgc
     2461 attgcgacgg cttgaaatgt ttcaatcgcc tgggcttgct tttccttggt cgggtcagcc
     2521 gccagttcca gcccacgttg atattgcttt tcgagggcaa cccgctggag gtcctgggcc
     2581 gtcgccgggt gaacttcggc cagggtaatc gtgaactttc cttcggaagg ctctttggcc
     2641 acgggggtga cctggaccag gtaggttcca gcccgttcaa gcaccagaaa aagctgctcg
     2701 gcttcaaaat ttgctggcgt cgagtttgtt tcagccacca gttgcccatc agccgtttgg
     2761 agtgtcacga caacatcgag actgatttgt tcgaccagaa ctttgaccag ggtgttggct
     2821 tccacggtca ggcggaactc gtgtttctcg cctgacttga gccccttttc cagtgggtga
     2881 ttcaggtcaa gcaatggagc ttgcctcttt gaagcagcca caccactcac tggagtcaag
     2941 ccaaaccaag ttgccagggc aaacaataag aaacaatcgc gcatatcact ccaccacatc
     3001 agattgaacc aggattagcc gctaccacag ggcacaccca actgcaccaa cgtaaaaaat
     3061 ttgtacagtg acgtggcata ttctttcagg taggaattgt tgacgaaaca cagcataggg
     3121 gtaaagaatc gctgcgtaaa tccttttgtg atttgcccgg catcgctggg tgaaacatcg
     3181 gcgattttca gaccttaccc cttagccaaa tccaaaaaac cgacggagca ttctatgttc
     3241 aaaccccgct ttggatattt ggcagtggtt gccattgcat tgcaattttg tggcaactcc
     3301 cttttcgcac aatcagttcc aggtgaaata ccaggacttc aaaaaacgag aaaaccagcg
     3361 aataaagtaa acctggggcc gaccaccggc ctgggagctg acaaacaggg gcgaatttat
     3421 attgtcagca acacaagaaa tcgagtctat cg
//
LOCUS       DAOQDO010000397         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20556_length_3446_cov_3.182542, whole genome shotgun sequence.
ACCESSION   DAOQDO010000397 DAOQDO010000000
VERSION     DAOQDO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20556_length_3446_cov_3.182542"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PKZ53_07670"
     CDS             complement(<1..768)
                     /locus_tag="PKZ53_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ40351.1"
                     /translation="MPKDKVLVVDDEQLIRWSLTEALNEWGYECVEAATASAAMTLFE
                     SEQPTAVLLDINLPDGSGLNVLRDVKRIQPQAVVIMITANVLVDDTIAALRAGAYDFI
                     GKPVNLEELQITIRNGIEAQRLRKEVTQIRREQAHQFSFEQIVGRSVAMMNTLELAKK
                     VAASEVSCVLLQGESGSGKDVIAKAIHYASRRSSEPFVAINCAALPSTLIESELFGYE
                     KGAFTDAKARKSGLFEQAEGGTLFLDEIGEMELNLQAK"
     gene            1332..1958
                     /gene="ribA"
                     /locus_tag="PKZ53_07675"
     CDS             1332..1958
                     /gene="ribA"
                     /locus_tag="PKZ53_07675"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNJ40352.1"
                     /translation="MNAFSEGYPVQSTVERIAEANLPTEHGDFRIIGFRSLTSDEEFV
                     ALTMGTPQADRPSLVRIHSQCLTGDVFHSIKCDCGRQLARAMEMIAEEGHGVIVYQHQ
                     EGRGIGIINKIRAYALQDQGADTIEANLRLGLEVDARDYTQCAEILQLLGLQKVRVMS
                     NNPEKISTLREAGLTVVERVPIESQPFEPSMNYLRTKKERMGHLLSLV"
     gene            2131..2364
                     /locus_tag="PKZ53_07680"
     CDS             2131..2364
                     /locus_tag="PKZ53_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40353.1"
                     /translation="MGKVHGIAKIRVTEENRFSTFVKSRKSEAYRDLEAWQRWFADRG
                     IPSIIASTPSGYALYRDGLVEVDIHDESVFQAA"
     gene            complement(2484..>3446)
                     /locus_tag="PKZ53_07685"
     CDS             complement(2484..>3446)
                     /locus_tag="PKZ53_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HNJ40354.1"
                     /translation="PPEELRQEAFTLAAHYGHRPREVQTILNELANRFGLDPETLREA
                     FYADLPENHMLVSLPDQTPEQLIDRYNLAQAQGLLYYARQIVIQAHRNVPGEYRKLFH
                     LIKFYGLMYAVEGNLDDGYRVFVDGPASLFRQNLKYGVKMAAFFPALLHITRWEIEAE
                     LKIKGVPATYHVTSHAALRSHYPKPPAYDSLLEAAFVERWDRQDTDWTLEREVEIVDL
                     KGTVMVPDFALRHPSGEVFHVEIVGFWHPDYLRRKFDKVRRSGMKNLILAVSDRLNVT
                     DDHLAGVVGPVIFFKGKLLPKAVLEVVEGLVGAKVEQSVGQDNE"
BASE COUNT          877 a          829 c          849 g          891 t
ORIGIN      
        1 tttggcctgc aggttgagtt ccatttctcc gatttcatcc agaaagagcg tgccgccttc
       61 agcctgttca aaaaggccgg atttgcgggc tttggcgtcg gtgaaggcac ctttttcgta
      121 cccaaacagt tcgctttcaa tcagcgttga gggtaatgcc gcgcagttaa ttgccacaaa
      181 gggttcgctg gaccggcgcg atgcatagtg gatggctttg gcgatgacat ctttgccgct
      241 gccggattct ccctgcagca ggacacagga aacttcgctg gcggctactt tcttggccag
      301 ttccagggta ttcatcatgg ccaccgaccg cccaacgatt tgctcgaaac taaattggtg
      361 tgcctgttcg cgtctgattt gggtgacttc tttgcgcagt cgttgggctt caatcccatt
      421 tcggatcgtg atttgaagtt cctcaaggtt gacgggtttg ccaatgaaat cataggcgcc
      481 agcgcgtaac gcagcgatgg tgtcatcaac caggacgttg gcggtaatca taatcacaac
      541 cgcctgaggc tggatgcgtt ttacgtcacg caggacattg agccccgatc cgtctgggag
      601 gttaatatcg agcagcacgg cggttggttg ttcagactca aacaaggtca tggcggctga
      661 agccgtggca gcttccacgc attcgtaacc ccattcattg agtgcttcag tcagtgacca
      721 gcggatgagt tgttcgtcgt caacaacaag aactttgtct ttaggcatga tggaatgata
      781 atcaatcgtt aagaaaaggt tatgggcaaa agcaggccca aagcatacat gattttattt
      841 tctgaggcga caaaaccttc tccaaactgg aatcatttgt caatgtttgc atctagattc
      901 aattgcgccg cgaagaaaag ggagtcgtcc caaagtttgt tctccctcaa atcaaaattc
      961 actgacgggc gacgctcccc ccaatctgat caacgatctc atcgtgatgc ccgtgaatct
     1021 acaaccttga ctgcgttcaa gaagcccaga ttgcagcgct ctggaacata aatctgatac
     1081 gaacgtgtgt tgaggttgga atagcacgtt tttgtctact ttctgtcaag gacaaaagaa
     1141 aattgtttag cttttgtcca ggaaaaaata ttgacaagac agaaagtatc gagtaccttt
     1201 cccaggctga atgagcattc acttctgtca tagaaggcca atggtcttgc tctcatataa
     1261 cttgatgatc tcgatgtacc tcgttgggaa gaaattctca accctgtgaa agaaaaagga
     1321 aaggacacga tatgaatgca ttttctgaag gctatccggt tcagtcaacc gttgagcgta
     1381 ttgccgaagc aaatttacca actgaacatg gagactttcg aattattgga ttccgaagtt
     1441 tgaccagcga cgaagaattt gtggcgctca caatgggaac cccccaggct gaccggccca
     1501 gtctggtccg aattcattct cagtgtttaa ctggggatgt atttcattcg attaagtgtg
     1561 attgtgggcg gcaactggcg cgggcgatgg aaatgattgc cgaagaaggt catggcgtga
     1621 ttgtgtatca gcaccaggaa ggccggggga ttggtattat caataaaatt cgcgcttacg
     1681 ccctgcagga tcaaggtgct gatacgattg aagccaatct gcggctgggg cttgaagttg
     1741 atgcccgtga ttacacccag tgcgctgaga ttttacagct tctcggcttg caaaaagtcc
     1801 gggtgatgtc caacaaccct gaaaaaatct cgacgctccg tgaagctggg ctcactgtgg
     1861 ttgaacgggt gccgattgaa agccagccgt ttgaaccatc aatgaactac ctgcgcacca
     1921 aaaaagagcg aatgggccat ttgttgagcc ttgtctgagt ttttggcgca caaattgcaa
     1981 gatatttttt tgaaatcaat tgcaacctaa atttgagcaa aaagctcaca ctgattgaaa
     2041 acgaagacat aaaagcgaaa tcttaaacat tatccggaac ggtttggtga ggattccccc
     2101 aaccagtttc gctcactctg gaggttacat atgggaaaag ttcatggcat tgctaaaatt
     2161 cgtgtcaccg aagaaaaccg cttctcaacg tttgtcaaaa gccgaaagtc cgaagcctat
     2221 cgtgaccttg aagcctggca gcgctggttt gccgaccggg gcattccgtc aattattgcc
     2281 agcacgccaa gtggatatgc tttataccgc gatgggttgg ttgaggttga cattcatgat
     2341 gaaagtgttt tccaggccgc ataacttatc aggtaacctg taggggcaaa aggactcacc
     2401 tggatctaac tccatcaatt cgctcttctt ttcgtgtcct tcggcttctt cgacgcaacc
     2461 atattggatg gttcttttgc tccctactcg ttatcctgcc ctaccgattg ttccacttta
     2521 gctcccacca atccttccac gacctcaagc accgcttttg gcagtaattt ccccttaaag
     2581 aaaataactg ggccgaccac accggcaaga tggtcatctg ttacattgag ccggtcagag
     2641 actgccagaa tcaggttttt catacctgat cgccgtacct tgtcaaattt ccgtcgcaaa
     2701 tagtctggat gccagaaccc cacaatctca acgtgaaaaa cctctccgga tggatggcgc
     2761 agggcaaaat caggcaccat gaccgttcct tttaaatcca caatttcaac ttcacgttca
     2821 agtgtccagt ctgtgtcctg ccggtcccac cgttcgacaa atgcggcttc gaggaggctg
     2881 tcataggctg gaggtttggg atagtgcgaa cgtaaggccg cgtgggaagt cacgtgatag
     2941 gtggcaggta cccctttgat ttttaactcg gcttcgattt cccagcgcgt gatatgcaac
     3001 aacgccggaa aaaatgccgc cattttgaca ccatacttta aattttgacg gaaaagactg
     3061 gctggaccat ccacaaagac ccgatagcca tcgtccaagt tgccctcaac cgcatacatc
     3121 aatccataaa atttaatcaa atgaaataat tttcgatatt cgccaggaac attgcggtgg
     3181 gcctgaatga caatttgacg ggcgtaatag agcaacccct gagcctgagc caggttgtat
     3241 cggtcaatga gttgttcggg tgtctggtcc gggagtgaaa ccagcatatg gttttcaggc
     3301 aaatcggcat aaaacgcttc gcggagcgtt tccggatcca gcccaaaccg gttggccagt
     3361 tcattcaaaa tagtctggac ttcacgtggt cggtggccat agtgggccgc cagggtaaag
     3421 gcttcctgtc ggagttcctc tggcgg
//
LOCUS       DAOQDO010000398         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20616_length_3440_cov_2.005022, whole genome shotgun sequence.
ACCESSION   DAOQDO010000398 DAOQDO010000000
VERSION     DAOQDO010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20616_length_3440_cov_2.005022"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKZ53_07690"
     CDS             <1..251
                     /locus_tag="PKZ53_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40355.1"
                     /translation="MQTLPENPIDALDRIQELMKITKTPRGFKTFKKFRQDRDMRIKG
                     LFEYIRNYSAHPPGTKIRRANGRTYAVQADGSFRRLPE"
     gene            260..712
                     /locus_tag="PKZ53_07695"
     CDS             260..712
                     /locus_tag="PKZ53_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNJ40356.1"
                     /translation="MSFLSLTRLQQIARGLYNLLATDIVNRSAAWEIRRRSGLTPTAA
                     GNLVSQITQYLKEYSTPDGYIKTVATGAVTTVFNEERDAALADADIARWGYSAILDTN
                     TCGPCRDADGMEGKLEDLPAVPNPECEGGLKCRCIHYAVLASEDAAKA"
     gene            735..1217
                     /locus_tag="PKZ53_07700"
     CDS             735..1217
                     /locus_tag="PKZ53_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40357.1"
                     /translation="MPIIRFADIYNEIHGSNSASFFGTFPTLDKDRHPVFEFTSDGNV
                     VFLGHVSGNVYFWPHPKTPSEKYARWRELKIMDADPGRLAALRRCVVLRWTKELTFIC
                     KYGDPRALYRFMVKEWGLRGCRASIEWAYRLGAKVPLPLKKAIKTLEKTERRKNGVAS
                     "
     gene            1201..1452
                     /locus_tag="PKZ53_07705"
     CDS             1201..1452
                     /locus_tag="PKZ53_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40358.1"
                     /translation="MAWQVEHLVKGDRISITEETRFSTFVDENNGDGFVTLCTWLDWF
                     RSRGKRAALVLTTSGYAVFREGLLPVGFDARKGIIKEVR"
     gene            1449..1661
                     /locus_tag="PKZ53_07710"
     CDS             1449..1661
                     /locus_tag="PKZ53_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40359.1"
                     /translation="MRCPACKSESTELLRTDYQEGTNRRRHQCKSCNFRFVTVQVYEV
                     QFKLLRSLEEWILKQDVAFLIALLPR"
     gene            1741..2838
                     /locus_tag="PKZ53_07715"
     CDS             1741..2838
                     /locus_tag="PKZ53_07715"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HNJ40360.1"
                     /translation="MANNKWAEFSNPQIGPDLQLRHKLYQLGDWEHAYFGKFEITPEL
                     HAEIVANFSADEPQNLNYNHVNDAISAGLIIEVEADTDADGWAWGTSLLTDAAAEYVR
                     KREYRGFSAEIDFKAKDRKGNPVGAYLCGGALTNTPFFNIVLSDRRLAASAYPTVDEL
                     QAATAAEIPGEPMDPLTSRYDAETETLFVTLPDGTEMPVMLPEGFGPMEDMADMKRKQ
                     TEAESQMAAQVAASRQMNDRLLALERANIELKTQVGKERELRLNAEADAFLTTQRVKP
                     SLREDVKEKYLLKGAEEARSFLSRLGDLAIVDEREHGVSATPELNDISFIDAEISKLQ
                     QSNPKMPLSQIHLSVAKACPAKFQRWEAGGM"
     gene            2838..3221
                     /locus_tag="PKZ53_07720"
     CDS             2838..3221
                     /locus_tag="PKZ53_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40361.1"
                     /translation="MNSLYLDGVFDSDCDQYLLCETDTDRNKGMKKASGANVRVTGVL
                     QKTVSGATSGVPITESVQVQGVTWVMLASGETVANGDPLKAINSSGHAGKAAVSTDKV
                     FGYSRQSGTGPCLISCYLDANSSYQ"
BASE COUNT          850 a          923 c          965 g          702 t
ORIGIN      
        1 aaatgcaaac tctcccagaa aatccaattg atgccctgga tcgcattcag gagctaatga
       61 aaatcaccaa gaccccacgc gggttcaaga cgttcaagaa gttccggcag gaccgcgaca
      121 tgagaatcaa gggtcttttt gaatacatcc ggaactacag tgcccaccca cctggaacaa
      181 aaatcaggcg ggcaaatggg cgaacttatg cagtccaggc ggatgggtct ttccggcggc
      241 tgcccgaata ggagaaacta tgtccttcct atcacttacc cgactccaac agatcgcccg
      301 tgggctttac aacctgctgg caactgacat tgtgaatcgc tcggcagcct gggagattcg
      361 gcggcggtcc gggctgacac caaccgcagc cggtaatctc gtgagccaaa tcactcagta
      421 cctcaaagag tactcaacgc ccgatgggta tatcaaaacc gtcgctaccg gcgctgtaac
      481 cacggtattc aacgaggagc gcgacgctgc ccttgccgat gccgacattg cccgatgggg
      541 gtacagcgcc atccttgaca caaacacctg cggcccgtgt cgcgatgccg atgggatgga
      601 gggcaagctt gaggacttgc cggcagtccc gaaccctgaa tgcgagggcg ggctgaaatg
      661 ccggtgtatc cattacgctg tcctggcgag cgaggacgca gccaaagcat aattaaacaa
      721 cggaaaggga gacgatgccg attatccgat ttgccgatat ttacaacgaa atccacggga
      781 gcaattcggc ctcattcttt gggacatttc ccaccttgga taaagaccgt catccagttt
      841 ttgagtttac ctcagatggc aacgtggtgt ttcttggaca cgtaagcggt aacgtttact
      901 tttggccgca ccccaaaact ccatccgaga aatatgcccg gtggcgagag ttgaaaataa
      961 tggacgctga cccaggacga cttgccgcac tcagacggtg cgttgttttg cggtggacaa
     1021 aagagcttac ttttatctgt aagtacggcg acccgcgtgc cctgtatcgg tttatggtta
     1081 aagagtgggg actgagagga tgccgtgcct caattgaatg ggcgtaccgg ctcggggcaa
     1141 aagtcccgct gccgctcaag aaggcgataa agaccctgga gaaaacagag aggagaaaaa
     1201 atggcgtggc aagttgagca tttagtaaag ggcgaccgca tttcgattac cgaagaaacg
     1261 cggttcagta ctttcgttga cgagaataac ggcgatggat ttgtaacgct gtgtacctgg
     1321 ctagactggt tccgtagccg tggcaaacgg gcagcccttg tgctcacgac cagcgggtat
     1381 gccgtgttcc gtgagggcct gttacccgtc ggctttgacg cccgcaaggg aataatcaag
     1441 gaggtgcgat gagatgccct gcgtgtaagt cggaatcaac cgaactactc cggactgatt
     1501 accaggaagg gacaaaccga cggcgccatc aatgcaaatc gtgcaatttc cggtttgtga
     1561 ccgtgcaggt atacgaggtc cagttcaaac tcttacgatc cctcgaagag tggatactca
     1621 agcaagatgt tgcgttcctg atcgccctgc tcccccggta gggctttttt ctgctatctg
     1681 tagcatcaag gatggcactt tttgccgcct gcctgggaac cgccccacca ttcgcccact
     1741 atggcgaaca acaaatgggc ggaattcagc aatcctcaga ttggtcccga tctccagttg
     1801 cggcacaagt tgtaccaact gggcgactgg gagcacgcct atttcggaaa attcgaaatc
     1861 actcctgagc ttcacgctga gatcgttgcc aatttctccg cagacgaacc ccaaaatctc
     1921 aattacaacc acgttaacga cgccatcagt gcgggcctga tcatcgaagt tgaggccgat
     1981 actgatgccg acggctgggc gtggggcacg tcgctactga ccgacgcagc cgctgagtac
     2041 gtgcgcaagc gagagtatcg tgggttctcg gcggaaattg acttcaaggc gaaggaccgc
     2101 aaaggtaacc cggttggggc ttacctgtgc ggtggtgcgc tgaccaatac cccgtttttc
     2161 aacatagtgc tctctgaccg acgcctcgcg gcatcggctt atcccaccgt ggatgaacta
     2221 caggccgcga ctgcggcgga aatccctgga gaacctatgg atccacttac ctcacgttac
     2281 gacgctgaga ctgagaccct gtttgtaacc ctgccagacg ggacggagat gcccgtaatg
     2341 ctgccggaag gcttcggccc gatggaagac atggcggata tgaagcggaa acaaaccgaa
     2401 gctgagtctc aaatggcggc acaggtggca gcgagtcggc aaatgaacga ccgactactg
     2461 gcactggagc gcgccaatat tgaactgaaa actcaggtcg gcaaagagcg cgagttacga
     2521 ctcaacgccg aggccgacgc attcctcacg actcagcgcg ttaagccaag cttgcgcgaa
     2581 gacgtcaaag aaaaatacct gctcaagggg gctgaagaag cgcgctcgtt tttgtcccga
     2641 ctgggcgacc tggccattgt ggacgagcgc gagcacggag tgagcgcaac tcctgaactg
     2701 aatgatattt ccttcattga tgcggagatc tccaagctcc agcagagtaa ccccaaaatg
     2761 ccactttcgc agattcacct gtcagtggcc aaagcctgcc ccgcgaagtt tcagcggtgg
     2821 gaagcaggag gtatgtaatg aactcgctgt acctcgacgg agtgtttgat tccgattgcg
     2881 accagtacct cctgtgcgaa accgacaccg accgcaacaa gggaatgaag aaagccagcg
     2941 gcgcgaatgt ccgcgtgact ggcgtcctgc aaaagacagt gagcggcgcc acatcgggcg
     3001 tccctatcac cgagtctgtt caggtccagg gcgtcacctg ggtgatgctt gcgtcaggcg
     3061 aaacggtcgc aaatggtgac ccgctcaaag caatcaatag cagtggccac gccgggaaag
     3121 ccgctgtttc gaccgacaaa gtttttgggt acagccgcca gtctgggacg gggccgtgcc
     3181 tgatttcctg ctacctcgat gcaaacagta gctaccaata agggggaata atggcacttt
     3241 cgacgaattc atatgtgtca aaggtaatga ccaacttcgt cagcgagcgg atgcaatcgt
     3301 atggcgatga tgggtttgtt gccggcaagt tgctcccttt tgttggagtg accggcaaga
     3361 ccggaacatt ttacacgcgt aaccggtggt ttgcgaaaga cctgccggcg aatcacattg
     3421 tgcgcgggtc aaagtcacca
//
LOCUS       DAOQDO010000399         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20627_length_3439_cov_3.209811, whole genome shotgun sequence.
ACCESSION   DAOQDO010000399 DAOQDO010000000
VERSION     DAOQDO010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20627_length_3439_cov_3.209811"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKZ53_07725"
     CDS             <1..787
                     /locus_tag="PKZ53_07725"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ40362.1"
                     /translation="PAGQTTYADTPPMADGTVPYFYALSATYNSTTESALSNEVAATP
                     GVNDTMAPVVEVFTPNGGSVVTTGDILEVTWSAEDNVGLASHDIDLSTDSGATFSVSI
                     ATGLEGDLRSFQYEVPDSVTTRTARIRVTAKDGAGNAGSDASDTDFIIRVPDSTPPTV
                     QVVSPNGGEKLKSGVAFNISWQATDESGIESQDVLLSTDSGATFPTQLATGLAGGTLS
                     FSFTPNVTGKVKTARVRVVALDTAGNSAQDDSNGNFQIKGKKK"
     gene            1008..2132
                     /gene="recF"
                     /locus_tag="PKZ53_07730"
     CDS             1008..2132
                     /gene="recF"
                     /locus_tag="PKZ53_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNJ40363.1"
                     /translation="MYLSQVESIGFRNLSGHLFGDAGVNILWGQNGHGKTSWLEVIAL
                     LGNTKSFRTHQLSEAINFHETAARAIAQVIHKSRTITLEVHLKGSQKQLLVNGKRVPV
                     SEYLGILDVFVYCREELSIVRGEPSERRRFLDRGVLSLKPGYVQTLADYNRVLKQKNA
                     LLKSIEETVPPPVEIFEMLDVWNAQLVEYGTQIHKARTAYVERLNQTLERRFFGAEAM
                     TVRYRSSLEGKGDLTHYPDLLAERLRLRREAELAAGHALVGPHRDDLDILTDGREVAR
                     FGSAGQQRSALLVLELAQLTMYHQEHDDFPVFLLDDIDAELDRDRIRTVLDFLQGKAQ
                     VFVTTSKAALAEEYRQRARLSQILSGTVVPQPEKALNAEF"
     gene            2198..>3439
                     /locus_tag="PKZ53_07735"
     CDS             2198..>3439
                     /locus_tag="PKZ53_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40364.1"
                     /translation="MSTPREEKELHTQSETTPNTPETYTSASITSLSGRDAVRLRPAM
                     YIGSNGELGLHHLVYEVCDNSVDEALGGYCTQVDVVIHPDNSITVLDNGRGIPVDIKE
                     DDLQKRSGVEIVLTELHAGAKFGEDNSYKVSGGLHGVGVSCVNFLSETFRVEVYRNNK
                     TYEQEFERGIPTGPLRQTGTTKRRGTKVTFKPDTSIFTFTEYNFDTLSQRLREKAFLT
                     KGLMITITDERSEVERRHEFLYKGGISEFVQHLNRNKNVLMPEPFYCESTQGEMTIEV
                     AIQYNDAYDEKVFSFANNINTIDGGTHLSGFRTALTASINSYAASEGLSKQLKENLTG
                     DDVREGLVGVISVKIPQPQFEGQTKNKLNSDVKGQVDSVIREHLKTYFEEHPAIAKRL
                     ILKAVEAARARGLTNGTVIWKH"
BASE COUNT          874 a          873 c          901 g          791 t
ORIGIN      
        1 cccagccgga caaaccacgt atgctgacac accaccgatg gctgatggaa cggtgccata
       61 tttttatgca ctgagcgcaa cctataactc caccactgaa agtgctctgt caaatgaagt
      121 tgcggcaaca cctggggtca atgacactat ggcgcccgtg gttgaggttt ttactccaaa
      181 cggaggaagt gtcgtcacca ccggcgatat tttggaagtg acctggagtg ccgaagacaa
      241 tgttggtttg gccagccacg acattgatct gtcaactgac agcggggcga cctttagcgt
      301 gtcaattgcg actggcctgg aaggagacct tcggtcgttc cagtatgaag tcccggattc
      361 agtcacgacc cgaacggccc ggattcgggt gaccgccaaa gatggggccg gaaatgccgg
      421 gtcagatgct tcagatactg attttattat tcgggtgccg gattcaactc cgccgacggt
      481 gcaggttgtt tcaccaaatg gtggtgaaaa actcaaatct ggagttgcct tcaacattag
      541 ctggcaagcc actgatgaat cagggattga aagccaggat gtcctgctgt caaccgatag
      601 tggcgccacc ttcccaactc aactggccac aggactcgca ggcgggactc tgtccttcag
      661 ctttacacca aacgtgacgg gtaaagtcaa aacggcccgt gttcgagtgg tggctttgga
      721 tacggctgga aattccgctc aggatgacag taatggtaat ttccagatta aaggcaaaaa
      781 gaagtagtct tcacccagga tgtatgtgag gggtgacggt gtcaaaaagg gaataagcca
      841 gccaggctta ttcccttttt tgtgtttttc gacagtgttt ttctgtgccg ttcagtctaa
      901 aatttgccct cgatctgacc ccaatacctt gtgttttcga agtctctgac tggacacaat
      961 tggccgtgtc tggtagactg gtatttcatt tacaatcaag aatttgtatg tatttatctc
     1021 aagtagaatc aattgggttt cgcaaccttt cgggacatct tttcggggat gcgggcgtaa
     1081 acattttgtg gggccaaaac gggcatggaa agaccagttg gctggaagtg attgcattgc
     1141 taggtaatac gaaatccttt cgcacgcatc agttaagcga ggccatcaat tttcacgaga
     1201 ctgctgcccg ggcgattgcc caggtgattc acaaaagccg gaccatcacg ctggaagttc
     1261 acctgaaagg ctcgcaaaaa cagttgctgg tcaatggcaa acgcgtcccg gtgagcgaat
     1321 acctggggat tctggatgtc tttgtctatt gccgtgaaga gttaagcatt gtccggggtg
     1381 aaccatcaga acgtcgccgc tttctggatc gtggcgtcct gagcctcaaa ccgggatatg
     1441 ttcaaacgct ggctgattac aatcgggtcc tcaagcaaaa aaacgcgctc ctgaaatcaa
     1501 tcgaagaaac ggttcccccg ccggttgaaa tcttcgaaat gctcgatgtg tggaatgccc
     1561 agcttgttga gtatggcaca cagattcaca aagcccggac cgcttatgtg gagcgcctca
     1621 accagaccct ggaacggcgg ttttttgggg ccgaagccat gacggttcgc tatcgctcgt
     1681 ccctggaagg gaaaggcgat ctgactcact atccagacct gctggccgaa cgattgcgat
     1741 tgcgccggga agccgaactg gctgccggtc acgccctggt tggcccgcac cgggatgatc
     1801 tggatatttt aaccgatggg cgtgaagtcg cccgatttgg aagcgccggt cagcaacgca
     1861 gcgccctgct ggtgctcgaa ctggcccaac tcaccatgta ccaccaggaa catgacgatt
     1921 tcccggtttt tctgctggat gacattgatg ccgaactgga ccgtgaccgg attcgcaccg
     1981 tgttggattt tctccaggga aaagcccagg tttttgtaac gacttcgaaa gcggcgctgg
     2041 ctgaggaata tcgccagcgg gcccggctca gccagattct aagcggaacc gtcgtgccac
     2101 agccggagaa ggcgttgaac gcagaatttt gagcagcacc attttgaccg taagggttcg
     2161 aactatattc acaacgtgat cgaggtttga ttgacacatg agtacaccga gggaagaaaa
     2221 agaattgcac acccaatctg aaacaactcc aaacacacct gaaacctaca cttcagcttc
     2281 tattacctcc ctgtcagggc gggacgccgt gcggttgcgc ccggccatgt atattggttc
     2341 aaacggcgaa ctgggcttac accacctggt ttatgaagtt tgcgacaact ctgtggacga
     2401 ggccctgggc ggatattgca cccaggttga tgtcgtcatt catcctgaca attccattac
     2461 ggtactcgac aacggacgtg gtattccggt tgacatcaag gaagacgacc tgcaaaaacg
     2521 atccggtgtg gaaatcgtgt tgactgaact acatgccggc gccaagtttg gcgaagacaa
     2581 ttcatataaa gtctcaggcg gattgcacgg cgtcggtgtg agttgcgtga acttcttatc
     2641 cgagacattt cgagttgagg tctatcgcaa caacaaaacc tatgaacagg aatttgaacg
     2701 gggcattccc actggtccgc ttcgccaaac cggtaccacc aaacggcgcg gtaccaaagt
     2761 cacctttaag cctgacacca gcatttttac ctttacggaa tacaactttg acacgctgtc
     2821 gcaacggttg cgtgaaaaag cctttttgac caaagggttg atgatcacca tcactgatga
     2881 gcgtagcgaa gtcgaacgcc gccacgaatt tttgtacaaa ggtggaattt cagaatttgt
     2941 ccagcacctc aaccgcaaca aaaatgtgct gatgcccgag ccgttttact gtgaatccac
     3001 ccagggcgaa atgacgattg aggtcgcgat ccagtacaac gatgcgtatg acgaaaaggt
     3061 gttcagcttt gccaacaaca tcaacaccat tgatggcgga acgcaccttt ccggcttccg
     3121 aacggcgctt acagccagca tcaacagcta tgccgcttcc gaagggctct ccaagcaact
     3181 gaaagaaaat ctgacgggtg atgatgtccg cgaaggactg gtcggcgtta tcagtgtcaa
     3241 aatccctcag ccccagtttg aaggccagac caaaaacaaa cttaacagcg acgtcaaagg
     3301 gcaggttgat tcggtcattc gcgaacacct caaaacatac ttcgaagaac atccggccat
     3361 tgccaaacgg ttgattctga aagcggttga agctgcccgt gcccgcggtc taaccaatgg
     3421 cacggtgatc tggaaacac
//
LOCUS       DAOQDO010000400         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20647_length_3437_cov_3.755174, whole genome shotgun sequence.
ACCESSION   DAOQDO010000400 DAOQDO010000000
VERSION     DAOQDO010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20647_length_3437_cov_3.755174"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKZ53_07740"
     CDS             <1..403
                     /locus_tag="PKZ53_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ40365.1"
                     /translation="DYITKPFSVRELIARVYAVLRRSEGESTAPVYDDGTLFIDYAHF
                     IVRCGGQEVNLTRKEFGLLKLLAQSKGRVLTREYLLDRVWGIDYDGETRTLDVHIRRL
                     RQKLGIEGYIETAVGIGYRFVEVRKPIVAAT"
     gene            427..1497
                     /locus_tag="PKZ53_07745"
     CDS             427..1497
                     /locus_tag="PKZ53_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40366.1"
                     /translation="MESSPNQYSPALMQAVLGSLREGILVIDQDTNIVLYNQAASEIF
                     KLRLQDSSGRLLRLVDATRNKTIHQGFRKVLQDGTPFEGEVEFLNSQTRTYSFRATPL
                     LLAPENTVNGAIGVFSDISQLVKLERVRREFFANLSHELRTPLTSILVYVETLLDGAI
                     YDIDYNVEYLQIINKHGKRMLNLVKDIADLSAIEGGEVSITPMDVDIRAFVENLATLV
                     SAELEEQNLTFTNAVPAGMRVLADTKSLEQILMNLIVNAIRFNRPKGSITVSARLEDP
                     EHAVITVNDTGIGMDAHHLPRIFERLYRVDKSRSQETGGTGLGLAIVKHLVHANQGDI
                     SVESTPNEGTSFHIKLLCPPKA"
     gene            complement(1975..2340)
                     /locus_tag="PKZ53_07750"
     CDS             complement(1975..2340)
                     /locus_tag="PKZ53_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HNJ40367.1"
                     /translation="MEEIKEYEDKSIQCVECGSDFVFTAGEQLFFADKGLKNTPKRCK
                     CCKIKKNERFNALLSAMSTDGSRPRIQVAVECAQCSVKTTVPFLPTQGRPVYCRDCFL
                     KMQPVELTPVAHLATPPAA"
     gene            complement(2976..3341)
                     /locus_tag="PKZ53_07755"
     CDS             complement(2976..3341)
                     /locus_tag="PKZ53_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNJ40368.1"
                     /translation="MSYRKLDVWNKSIELVIAVYQLTKSLPPEERFGLASQIQRSAVS
                     VSANIAEGYGRSTRGEYLQFLSMANGSLKETETLLTILVKVDLVSREQAFDAWKLAQT
                     AGKMLTRLILSLKQPKPIK"
BASE COUNT          870 a          804 c          824 g          939 t
ORIGIN      
        1 tgactacatc accaaaccat ttagtgtccg tgagctgatt gcccgggtct atgccgtttt
       61 gcgtcggagc gaaggtgaat ccaccgcacc ggtctatgac gatggaacct tgtttattga
      121 ttacgcccat ttcatcgttc gatgtggtgg gcaggaagtc aatttaacac gtaaggaatt
      181 tggattgttg aagttactgg cacaaagcaa gggacgtgtc ctgacacggg aatatctgtt
      241 ggatcgtgtc tggggcattg attacgacgg cgaaacccga accctcgatg ttcacatccg
      301 acggttgcga caaaagctgg gcattgaagg ctatattgaa acggcagttg ggattggcta
      361 tcgctttgtt gaagttcgca agccaatcgt cgccgcaacc tgatttccca cccacccata
      421 tccatcatgg aatcgtcacc aaaccagtat tcaccagcct tgatgcaggc ggtgcttggc
      481 tctcttcggg aaggtattct ggttattgac caggatacca atattgttct ctataaccag
      541 gctgcctccg agattttcaa actccggttg caagattcat ccgggcggct cttgcggctg
      601 gttgatgcca cccgcaacaa aaccatccat cagggatttc gcaaggttct tcaggacggc
      661 accccgtttg aaggtgaagt cgaattcctc aatagccaga ctcgaacata cagttttcgg
      721 gcaactccgt tgctgttagc tccggaaaat actgtcaatg gggccattgg ggtgttttct
      781 gatatttcac aactggtaaa gcttgagcgg gtccggcgcg aattttttgc caatctgtca
      841 cacgaactac gcacgccgtt gacatcaatt ttggtctatg tcgaaacact gctggatggg
      901 gcgatttatg acattgacta taatgtcgag tatctccaga tcatcaacaa acacggcaag
      961 cgcatgctca acctggtcaa agatattgct gatctttcag cgattgaggg cggggaagtc
     1021 tcaattacgc cgatggatgt ggacatcaga gcgtttgtcg aaaatctggc aacgctggtt
     1081 tcagccgaac tcgaagaaca aaatttgacc ttcaccaacg ctgtgccggc tggtatgcgg
     1141 gtcctggctg ataccaaaag cctggaacag attctgatga acctgatcgt gaatgccatc
     1201 cgatttaatc gccccaaagg cagcattacc gtgagtgcgc gcctggaaga ccctgaacat
     1261 gccgttatca cggtcaacga taccgggatt gggatggatg ctcaccattt gccgaggatt
     1321 tttgaacgac tctaccgggt tgataaatca cgttcccagg aaactggtgg tacgggtctg
     1381 ggattggcga ttgtgaaaca cctggttcac gctaaccagg gtgacatctc ggttgaaagc
     1441 acccccaacg aaggtacttc gtttcacatc aagttgttgt gcccgccaaa ggcgtgaaga
     1501 atgataccag tcagtcgtca gtagttagtc gtcagtagtt cgctaagttt atgtggttga
     1561 gtcgcttgac tgtttggtga tccgcgaact tcattgcagc tttggagaag cctggtcctg
     1621 gtgcttttcg aaaagtttgt tcttcagccc gaaatcttca acccccagcc cgaaatcttc
     1681 agccctgagc ccgaggattt tgcagtctga tgttttcatg aaaaaaggct gtgtgagtcg
     1741 ctcatcgcgt tcacacagcc tatggctgca atatcatttc tgcacgcctc tcccttcaat
     1801 agagttaact tgcaccacgt agccttgatg aaccccttag gagaagaacc ccgtgttcga
     1861 ccttggaccc attgtgcctt ttgcttacct tggcgtgccc gcttgtaaag atcagtgggt
     1921 gaactgcaaa aaatgtcacc cgagttgtac gtcaaagggt tagcctgggc gggtttacgc
     1981 cgcaggagga gtggctaaat gcgcaactgg cgtcagttcg accggttgca ttttcaagaa
     2041 acagtcacgg caataaaccg gacgaccctg cgttggcagg aagggaactg tggttttgac
     2101 actacattga gcgcattcga ccgccacctg gatccgggga cggctcccat cagtgctcat
     2161 ggcggacagc agtgcgttaa agcgttcatt tttcttaatc ttgcaacact tgcagcgttt
     2221 gggggtattt ttcagtcctt tatctgcaaa aaacaattgc tcaccagccg taaatacaaa
     2281 atcacttcca cattcgacac actgaattga tttatcttcg tactctttga tctcttccat
     2341 aatgttcttc gactcctcta atgactataa ggcgcttttg ctcacacgat aagtgcagag
     2401 cagggtgtag acacgtgcaa acgggcagac ttcgtcccca gatcgccatc tgaaacctgt
     2461 ttgaaaacaa aaaacaggca gacttcgacc caggcattca gacccccgtc tgaatacctt
     2521 tccgattccc cctggctcga tttgtgtttg gattactaaa gagtgaaaag gtgtctattg
     2581 acaatcattt atgtatccga agttaaagtg attgtcaagc attttgtctg gcgcttttcc
     2641 ccagtttgac ccgtcatgca ccgcaactga ttgatttttc aataagaaag aggttgcaga
     2701 gagtaacacc tttttttctc gatatttagt cttgatgacc tagtgtaaca cttgagattt
     2761 gaagaaaaat gaattttcca gtgttctgaa ctggaaaaaa ccagggctca gggcttgggg
     2821 ctgaagaaaa cgggctgaag aatatcgggc tgaagaatat cgggctgaag aatatcgggc
     2881 tgaagaaaac gggctgaaga acttggattc aataccctga gcccattttc ttcagctcca
     2941 tacgcttcag cctattttct ccctggggtc tgggcttact ttattggttt tggttgtttt
     3001 aaagacaaga ttaaccgagt caacattttt cctgctgttt gagcaagctt ccaggcgtca
     3061 aaggcttgtt cacgtgatac caggtcaacc ttcaccaaaa ttgtaaggag tgtttcagtc
     3121 tcctttaatg atccatttgc cattgaaaga aattgcaaat attcccctcg ggttgatcgt
     3181 ccgtaccctt ctgcaatatt ggctgaaaca gagactgctg agcgttgaat ttgactggct
     3241 agcccgaatc tttcttctgg tgggagggat ttggttaatt ggtaaacagc aatgaccaat
     3301 tcgattgact tattccaaac gtcaagtttg cgatacgaca tagcgataaa actcctgagc
     3361 atcaaattta gtttggaatg aagagaccgc aagtttggta ccaaaacaaa atttaaattg
     3421 atttttaaag ctcttta
//
LOCUS       DAOQDO010000401         3436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20663_length_3436_cov_3.228926, whole genome shotgun sequence.
ACCESSION   DAOQDO010000401 DAOQDO010000000
VERSION     DAOQDO010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20663_length_3436_cov_3.228926"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..1963
                     /locus_tag="PKZ53_07760"
     CDS             308..1963
                     /locus_tag="PKZ53_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ40369.1"
                     /translation="MKSKVYVTFGAVILLATISGGWWFDHHQSSIAEANTGKSEATSL
                     NRIVRTFTEAQEVINQHYVEEVTADDLTKTSIQGMLRTLDPHSNYFDTKEFQKIQSEQ
                     RGQFYGIGVSINRRNNRVFILATIENTPAVKAGLRYGDAILAVNGKSAIDWTTDQVMD
                     EVRGPQGEPVEVTVERVGVPKPLTFKIIRDAVPLPSIRHHFMIRPGIGYIALLQGFTH
                     TTEDELRVALQDLQAQGMRSLILDLRGNPGGLLNQAIKVASAFLPEDLTILSQKGRPG
                     NEEAEFREYRSENDRPNQSAMVVLINRGSASASEIVAGAIQDHDRGLIVGEQSFGKGL
                     VQTVFKLPFGSALTLTTAKYYTPSGRCIQRQYSGGALYDYYAHQPGTSQPVGQALSTD
                     MGRKVYGGGGITPDVEEKEQILTPIQAQLFSATFEFTRQLIGGQIQGFPQFRVTQTQY
                     HHTLRDDDYVVTDKLLTAFRAFLRTSPDYKSVTDSQVTENSKYLRNLIREQVVTAAYG
                     TEAGMEVRLQVDTQTQKAIETIPQARELAQRAQVARGGRTMVE"
     gene            2232..>3436
                     /locus_tag="PKZ53_07765"
     CDS             2232..>3436
                     /locus_tag="PKZ53_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40370.1"
                     /translation="MRFTMASCNQWYVLWLIRYGLTLGFLLLTGASAQAQPILRFEHF
                     REVQGLTSGAVRCLAQDSTGFLWVGTERGVFRYDGITFKYYPLHSTPLEPNITTNCLL
                     TDQAGGLWVGTQREGIFVYHQTDDTFVKLESLTQVGVKDQILSLFQDSSGRIWIGTRR
                     GLVGYNPETRSDQLFQVIQGEARAVLAVAEDAQKRLWVATTVGLFQFDPQTGTYRPHD
                     IGATSTRPVREISRLCFHPSGYLFVGTQDGLWSVKTDSSDLPTTLVDQVSQTALIQPC
                     HITSLLALNQTTLLVGGQDNGLAQVDIETGTTRWSTHDDRVGESLSSNSVTTLLLDRF
                     GVLWVGNGVTGLDKHSAYKHVFRLYRHNPFDEKTISGNYIRGICEDQDGFVWVCTQFN
                     GLNRIDLQTG"
BASE COUNT          842 a          802 c          930 g          862 t
ORIGIN      
        1 actgaaaacc aaagaggggg cagccaatct tttttcgttt gatgcggtgg tcaccgatga
       61 agggctgcgt ggagaggctg aactcacctg ttttaatcca cgggccgagg ccagggaaac
      121 caattttgtc gtgactcgct ccaaataagc gctgaagttg attcctcttc atcctggcgc
      181 cagatccggg aaaagatatg cggaatcttt tgacaaccgg aattcttttc ctttaagttt
      241 gtggaagaaa agctgttgtt cggtggtgtc caacacggtt gagtttgttt gtgaaaggga
      301 gcagatcatg aaatcaaagg tatatgtcac gttcggtgcg gtgattttac tggcaacgat
      361 cagtggtggg tggtggtttg atcatcatca gtcgtcaatt gctgaggcga acactggaaa
      421 atccgaggcc accagtttaa atcgcattgt tcggacgttt actgaagccc aggaagtgat
      481 caatcagcac tatgtggaag aagtaaccgc tgatgatctg accaaaacct cgatccaggg
      541 catgttgcga acccttgatc cacattcaaa ctattttgac accaaagaat tccaaaaaat
      601 ccaaagcgaa cagcgtgggc agttttatgg tattggtgtc tcgatcaatc gacgcaataa
      661 ccgggtattt atcctggcca caattgaaaa taccccggcg gtcaaagcgg gactgcgcta
      721 tggcgatgcc attttggcgg tgaacgggaa atcggcgatt gactggacga cggatcaggt
      781 gatggatgaa gtccgggggc cgcaaggcga accggtcgaa gtaaccgttg agcgggtggg
      841 cgtccccaaa cccttgacct ttaaaatcat tcgcgatgct gtgccgctgc cttcgattcg
      901 ccaccacttt atgattcgtc ctggaatcgg gtatatcgct ttgttacaag ggtttactca
      961 tacaaccgag gatgagttgc gggtggcgct tcaggattta caggcgcagg ggatgcggtc
     1021 gctgatcctg gatttacgtg gcaatccggg aggtctcctc aatcaggcca tcaaggtggc
     1081 cagcgctttt ttacctgaag atctgaccat cctgtcacag aaagggcgac ctggaaacga
     1141 agaagctgag tttcgtgaat accgatctga aaatgatcgg cctaaccaga gcgcgatggt
     1201 ggtcttaatc aaccgtggtt cagcctcagc ctctgaaatt gtggctggcg ctatccagga
     1261 ccatgaccgg ggattgattg ttggggaaca aagctttggc aaaggtctgg tccaaaccgt
     1321 ttttaaatta ccctttggat ctgccctgac tttgacgacg gccaagtact ataccccaag
     1381 tggtcggtgt atccagcgtc agtattctgg gggggcactc tatgactatt atgcccacca
     1441 gcctggaacc tcccagccag ttggccaggc cctttcgacc gatatggggc gtaaagttta
     1501 cggtggcggt ggtattacgc ctgatgtcga agaaaaagaa caaatcttga cgccgattca
     1561 ggcgcagttg tttagtgcca cgtttgagtt tacgcggcaa ttgatcggcg gccagatcca
     1621 gggtttcccg cagtttcggg taacccaaac ccagtatcat catacactcc gggatgatga
     1681 ctatgtcgtg accgataagc ttctgacggc gtttcgggcg tttctccgca ccagcccgga
     1741 ctacaaatct gtcactgata gccaggtaac tgaaaacagc aaatatttgc gtaatctgat
     1801 ccgcgagcag gttgtgacgg ctgcctatgg tacggaagcc gggatggaag tccggctgca
     1861 ggttgatacc caaacccaaa aggccattga aacgattcct caggcacgag agctggccca
     1921 acgcgcgcaa gtggcacgag gcgggagaac aatggttgaa taggcagggg ggaaacatca
     1981 aacaggcaca cctgagaaga taagaaaaag agagatggtg catctctctt tttttggagt
     2041 atgttgcgtc tgataattat taatataaag taggtttatc agtgctctga gcgaagattg
     2101 ggggaattga atcccactga taggaatatt ctcgtgttta agatacactg atccccattc
     2161 attcctcatc attgccccgc atcctggatt gtgttacctc agttcatctg actttgcctg
     2221 ccaaccccca catgcgattt accatggcct cttgcaatca atggtatgtt ttgtggttga
     2281 tcagatatgg tctaaccctt ggtttcctgc ttctcaccgg tgccagtgcc caggcacaac
     2341 ctatcctccg gtttgaacac ttccgagagg tgcaggggtt gaccagcggt gccgtccggt
     2401 gtctggctca ggattcaact ggttttttgt gggttgggac tgaacgaggc gttttccggt
     2461 atgatgggat aacgtttaag tactatccat tgcactcaac accgcttgaa ccaaatatca
     2521 ccaccaattg tttgctgact gaccaggccg gagggctgtg ggttggaact cagcgcgagg
     2581 ggatatttgt ctatcatcaa accgacgata cgtttgtcaa actcgaatcc ctgacacaag
     2641 ttggtgtgaa agaccagatc ctttcgctct ttcaagactc atccgggcga atatggattg
     2701 gaaccaggcg gggattggtg gggtacaacc ctgaaaccag gtcggatcag ctctttcaag
     2761 tgattcaagg tgaagcccgg gcggttctgg cagtggctga ggacgcacaa aaaagattgt
     2821 gggttgccac caccgtgggg ttatttcagt ttgatcccca aaccggaaca tatcgtccac
     2881 acgatatagg ggccacctca acccggccag ttcgtgaaat cagccggctt tgttttcacc
     2941 catctggata tttgtttgtt gggactcagg atgggttgtg gtcagtgaaa actgattcaa
     3001 gtgaccttcc aacaaccctg gttgatcagg tgagccagac cgccctcatt caaccctgtc
     3061 atatcaccag tctgctggct ctaaaccaaa ccaccctgct ggtcggcggt caggataacg
     3121 ggctggcgca ggtagatatt gaaaccggaa caacccggtg gagtacccac gatgaccggg
     3181 ttggtgaaag tctctccagt aatagtgtga cgaccttatt gctggatcgg tttggggtat
     3241 tgtgggttgg gaatggggtg accggattgg acaaacattc tgcctacaag catgtatttc
     3301 gcctgtatcg gcacaatcca tttgatgaaa aaacaatcag tggaaattat attcgcggca
     3361 tttgtgaaga tcaggacggg ttcgtctggg tgtgcaccca gtttaacggc ttaaaccgga
     3421 ttgatcttca gactgg
//
LOCUS       DAOQDO010000402         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20672_length_3435_cov_2.936391, whole genome shotgun sequence.
ACCESSION   DAOQDO010000402 DAOQDO010000000
VERSION     DAOQDO010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20672_length_3435_cov_2.936391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKZ53_07770"
     CDS             <1..1003
                     /locus_tag="PKZ53_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNJ40371.1"
                     /translation="SAHGRIQVGGKERVQFLNGLVSNYIKPLQHGFGVPALFLTAQGK
                     VIADCHIFGVDETFWIDLDATLAEKIYKSLFRFTFAGDFKVEDLAKSYGLLSLQGPQA
                     SALLAAITGDTPHEWPVTHQVDKVGGGTEDIPVLPLKKLQLGKTEALVVRRARGTVDG
                     YDLYIHREALAQTWNWLTSQGTAFHLRPVGWEALELVRVEAGLPRYGVDVDETVIALE
                     ANLGSAVSYHKGCYVGQETVAKIHWRGHDQVARKLTQIVVEGTNLPQRGSRIVKGEKE
                     VGTITSAIYSPQLGDAILALGYVRSSVIQAGGTFTIKNDDGTEVSAKLKDSGLKTLG"
     gene            1260..1580
                     /locus_tag="PKZ53_07775"
     CDS             1260..1580
                     /locus_tag="PKZ53_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40372.1"
                     /translation="MSHPEYPEPSAPLNHQPDIEWATAEDAYHIINHLLTYVHATSDL
                     IALMATALGEAQVKKLAETHPWADYLAARRMLEQIQPEIQRFSQTMIELAKDRPDRPD
                     PEEE"
     gene            complement(1759..2277)
                     /locus_tag="PKZ53_07780"
     CDS             complement(1759..2277)
                     /locus_tag="PKZ53_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40373.1"
                     /translation="MPAIMSLGLGGALPGEPTKSEILDHLAAFAAHDFISNFYEIVEG
                     ELYQISRENGETKITLVHPEETLPGWSPYQCIERYTYPEWYQAMFAAWNSRPGGDITY
                     RDWSNVEYSVFVPFYEGRPRWVSVPPLTDKLGVIGVEAGICIGQELDLPDYFIRTLDR
                     ANEFVFLARISG"
     gene            complement(2423..>3435)
                     /locus_tag="PKZ53_07785"
     CDS             complement(2423..>3435)
                     /locus_tag="PKZ53_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ40374.1"
                     /translation="PQSPEPAGKFVPTPEYFEQLRQYFRRENFGITKVDILKGNVGYV
                     DFKYFAPPEIAAETYAAVMGYLANTDALIIDVRASNGSMSPDAIPMLCTYFFEQPVHL
                     NDIYWRDDDSTRQFWTWAYVPGKRYVNKPMYVLTSGKTFSGAEEFAYDLKNLKRATII
                     GDPTGGGANPGGPRQANEHFSVWIPYGRAINPITKTNWEGVGVEPDIKIAPIQALHTA
                     HTLALKELVSKATDPGWKENLTAALAELEENPVKVKTVTLTLKGFDSARSIAVAGSFN
                     FWAPRANPLTRQGDTWVAKVEVTPGRHTYKFVVDGKWILDPGNPKTITDGQYSNSVLV
                     VE"
BASE COUNT          842 a          900 c          895 g          798 t
ORIGIN      
        1 aagcgcccac gggcgaatcc aggtcggtgg aaaagagcgc gttcaatttc tgaatgggct
       61 ggtcagcaac tatatcaaac ccttacagca cgggtttggg gtaccggcgc tctttctgac
      121 cgcccaggga aaagtgattg ccgattgtca tattttcggt gtggatgaaa ctttctggat
      181 tgatctggac gccactctgg ctgaaaaaat ttataaatcc ttgttccgct ttacctttgc
      241 tggtgacttc aaagttgagg atctcgccaa aagctatggc ttgctttctc tgcagggacc
      301 acaggccagc gccttgctgg cagctatcac aggggacact ccacacgaat ggcctgtaac
      361 ccatcaggtt gataaagttg gcggtggcac ggaagacatt ccggtattgc cgctgaagaa
      421 gctccaactt ggtaaaaccg aagcacttgt ggttcgacga gcacgtggga cggttgacgg
      481 atacgacctc tatatccacc gtgaagcgct ggctcaaacc tggaactggc tcaccagtca
      541 gggcaccgcc tttcatttgc gcccggttgg ttgggaagca ctggaactgg tgcgggttga
      601 agctggcctt ccccgctatg gtgttgatgt ggatgaaacc gtgattgccc tcgaagccaa
      661 cctgggttcg gcggtcagct accataaagg atgctatgtc ggccaggaaa ccgtcgccaa
      721 aatccactgg cgcggacacg accaggttgc ccggaagttg actcagattg tcgttgaagg
      781 caccaacttg ccacagcgtg gcagccggat tgtgaaaggt gaaaaagaag tcggcacgat
      841 caccagtgcc atttattcgc cgcaacttgg ggacgctatt ttagcacttg gatatgttcg
      901 ctcatcggtc attcaggctg gcggaacatt caccatcaaa aacgatgacg gcacggaagt
      961 cagcgcgaaa ctgaaagaca gcgggctgaa aaccttgggc tgaagacttc gggctgaaga
     1021 cctcgggctg aaaaccttgg gctgaagact tcgggctgaa gacctcgggc tgaagacttc
     1081 gggctgaaga ctcgcaagct cggggcggaa aaaaacgggt tcaataccct cagcccaact
     1141 tcttcagccc caagccctga gcccgctttc ttcagccctg agcccgactt cttcagccct
     1201 gagcccgctc ttcagcccga gatcacctgc catttacttt tttatcattg gggattttta
     1261 tgtcgcaccc tgagtatcca gaaccgtcgg caccactcaa tcaccagcca gacattgaat
     1321 gggcaacggc tgaagacgct tatcatatta tcaaccatct gctgacctac gttcacgcca
     1381 ccagcgattt gattgccctg atggcaaccg cccttggcga agcacaggtg aaaaagctgg
     1441 ctgaaacgca tccctgggct gactaccttg cggcacggcg gatgttggaa cagattcagc
     1501 ctgagattca aagattttct caaaccatga tcgaactggc caaggaccga ccagaccgac
     1561 cggatccaga ggaagaataa cgggttccgc gcttccgggt tccgcgcttc cgggttccgc
     1621 gcttccgggt tttcgaaagg gaaaggacaa aaagaacaaa agggacgaaa aggacataaa
     1681 tgcgacggga cgcaggtttg ttgaatcagt tccctttcga aaacccggaa cccgcaagca
     1741 cggaacccaa aagcagcttt agcctgaaat ccgtgccagg aaaacaaatt catttgcccg
     1801 atcaagcgtt cggataaaat agtcgggcag atccagctcc tggccaatac aaatcccggc
     1861 ttcgactccg atgacaccca gtttgtcagt cagtggcgga acactgaccc aacggggccg
     1921 accttcataa aacggaacaa acactgaata ttcgacattt gaccagtcgc gataggtgat
     1981 gtcgccgcca gggcgcgaat tccaggccgc aaacatggcc tgataccatt cggggtacgt
     2041 gtaacgctca atgcattgat atggactcca ccctggaagc gtttcctctg gatggaccag
     2101 cgtgattttg gtttccccgt tttccctgga aatctgatac agctcaccct caacaatttc
     2161 ataaaagttg ctgatgaaat catgggccgc aaaggcagcc aggtggtcta aaatttcgga
     2221 tttggtgggt tcgccgggta aggcgccgcc taatccaaga ctcataatgg ccggcatatc
     2281 gtttttctcc tgattctgac aaatcttgtg tggtgtgaac caaacaccgg aatgttgaat
     2341 caagccctgg cagagtgcaa actcattctt gtaagatctt gaaagtaagt cacataaccc
     2401 attgttcaac cttgaacaat ttttattcaa ccaccagcac tgagtttgaa tactgtccat
     2461 cagtgatggt ctttgggttt ccaggatcaa gaatccactt tccatccacc acaaatttgt
     2521 aggtatgtcg cccaggagtg acttcgactt tggccaccca ggtgtcaccc tggcgcgtga
     2581 gcgggttggc gcgcggcgcc cagaagttaa aactcccggc gacggcaatt gaccgggcag
     2641 agtcaaaacc tttgagcgtc agcgtgacgg ttttgacttt gacaggattt tcttcgagtt
     2701 cggcgagcgc ggcggtgaga ttttctttcc agccgggatc cgtggctttt gaaaccaatt
     2761 cttttaaagc cagtgtgtga gccgtgtgaa gcgcctgaat cggagcgatt ttgatgtctg
     2821 gttcgacgcc aaccccttcc cagttggttt tggtaatcgg attgatcgcg cgaccatagg
     2881 gaatccagac tgaaaaatgt tcattggcct gacgtggccc acccggattg gcaccacctc
     2941 cggttgggtc gccgatgatg gtggcacgct tgaggttttt cagatcgtag gcaaattctt
     3001 cagctccgga aaacgttttc ccactggtga gaacatacat cggcttgttc acatagcgtt
     3061 ttcctggaac gtacgcccag gtccagaact ggcgcgtgct gtcatcgtcg cgccagtaaa
     3121 tatcattgag gtgaaccggc tgttcgaaaa agtaggtaca caacatcgga atcgcatctg
     3181 gtgacatgct gccgttgctg gccctgacgt caataatcaa ggcatccgtg ttggccagat
     3241 accccatcac cgccgcatag gtctccgccg caatttccgg cggagcaaaa tacttaaaat
     3301 ccacatagcc gacattgccc ttgagaatat ccactttggt gattccaaaa ttttcacgac
     3361 ggaaatactg acggagttgc tcaaagtact caggtgttgg gacaaacttt ccggctggtt
     3421 ctggtgactg aggct
//
LOCUS       DAOQDO010000403         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20676_length_3434_cov_4.876591, whole genome shotgun sequence.
ACCESSION   DAOQDO010000403 DAOQDO010000000
VERSION     DAOQDO010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20676_length_3434_cov_4.876591"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="PKZ53_07790"
     CDS             complement(<1..2272)
                     /locus_tag="PKZ53_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40375.1"
                     /translation="MEGGAQVEIKGKNFTPDTVVVVGDAVATNTQVIKGKKIKFTVPP
                     QKAPGVRNLSVWTPNGIAQMPFQIIPKKLDELADGEVTTIAGGIEYLGDGDNQQNAIL
                     NRPTRIVADSIGNLFIADTYSHRIRRIDAKTGTITTIAGTGRQGFDGDNGPALAARLA
                     YPLSLALSSKGTLFFLDLGNQRVRQINLKSGIISTVAGNGRRGFSGDNGPAQAASLSV
                     GGLFSDFQKSGGIAVDQAENLFIADRENQRIRRVDAESGIIKTIVGTGIVEFSGDGGP
                     ASQADIFLPTNVAIDPNGNLFIADSGNNRIRRVDARTNIITTVAGNGAPGSRGDGGPA
                     LEAATEADSVVLDQSGNLFILEQFPRRIRRVDNISNTITTIAGNGNPGSQDSENGQNA
                     TQVGLGVKIDGGMNVDENGDILIVEPELNRIRKVVVQTGTIFSLIETQLDQIGDNGPA
                     INAIFQFRPYREFVDPSEDRRGAITVSQDGSLIIVDSGHHRVRKVDLSTNKISTIIGT
                     GISGFNGDNQLAGRTLLSEPSAVAGDSAGHFLIADYGNQRVRRVDAQTGIVTTVAGNG
                     SDKLNADYDEDYNEYSFLDDGGPAVEASIPNPRGIALDEAGNLFISNFGLFIRRVDAQ
                     TQIITTRLGCCSGSNSRSLRGLAFSPGGILYAANANRGIVKLEPNATTPTVLFFVKPF
                     FDVAVNRDGIVFAPVMTEHRIYRIRQPVELLTPIAGGKFGYEGDNGPATEAAFSSPSA
                     VAIDGANNLYIVDSGNN"
     gene            complement(2486..>3434)
                     /locus_tag="PKZ53_07795"
     CDS             complement(2486..>3434)
                     /locus_tag="PKZ53_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40376.1"
                     /translation="CENQTLGQYARDERQRNHLFFRYATNRSNITRYFFTKKAVFFNN
                     LKEDRGSNPMLVAGTPVNGIIEGNDENGVPTTLMFVNDAPHPKLGNNPTLPDEIDNIP
                     FVRIKVLDANTGKMVSNVTAQGILANEKAVALDVLKLEDLYVLAWAGIDGKPGNYANG
                     HLEVLKYLTGNFEKVNPTTTADFENPGWEPVLTSYYSLDRNFTTNRFRDDLGVDQVPN
                     VILQATVDTTSTFLTFKPDSTDSRLQLAGFSPTSTKKIYDLGVRCPAKIIGTVDNLNL
                     KLPNGVSVSPGSTFVLLENNGLISFLRLSPKGTSTYVGH"
BASE COUNT          884 a          816 c          732 g         1002 t
ORIGIN      
        1 cattgtttcc tgaatccaca atgtaaagat tatttgcccc atcaatcgcc accgctgatg
       61 gagacgaaaa agccgcttcc gtggctggcc cattgtcgcc ttcgtagcca aacttgcctc
      121 cagcaattgg agtgagtaat tcaactggct gcctgatccg gtaaattcgg tgttcggtca
      181 tcactggagc aaacactatg ccgtcgcggt tcacggccac gtcaaaaaat ggcttcacaa
      241 aaaacaacac ggttggtgtt gtggcattcg gctccaactt cacaatcccc cgattggcat
      301 tcgccgcata caggatgcca cctggactaa atgccagtcc ccgcaatgat cggctgttgc
      361 ttccagaaca acagcccaac cgggtggtga tgatctgggt ttgggcatca acccggcgga
      421 taaataaccc aaaattggaa ataaataagt tcccggcttc gtccagggcg attccacgtg
      481 ggttgggaat ggatgcttcc acggctggcc cgccatcatc taaaaaggaa tattcattat
      541 agtcctcgtc atagtcggca ttgagtttgt ctgatccatt ccccgcgaca gttgtaacaa
      601 tacccgtttg agcgtccacc cggcggactc gctggtttcc ataatcagca atcagaaaat
      661 ggccagccga atcacctgct accgctgaag gctctgaaag aagtgttctt cccgctaatt
      721 ggttatcccc attgaagcca gaaatgccag tgccaataat ggttgagatc ttattggtgc
      781 tcaaatcgac ctttcgaact ctatggtgac cagaatcaac tataatgaga cttccatcct
      841 gactaactgt aattgcacct cttctgtctt cagaaggatc aacgaattct cgataaggtc
      901 gaaactgaaa aatggcattg attgcgggtc cattatcgcc aatttgatcc aattgagttt
      961 cgattaaaga aaaaattgtt cctgtttgaa ctacgacttt gcgaattcga tttagttcgg
     1021 gttcgacaat cagtatatct ccattttcat caacattcat tcctccatct atttttactc
     1081 ctagcccaac ctgagtagca ttctgcccgt tctctgagtc ttgcgatcca ggattgccat
     1141 ttccggcaat ggtagtaatg gtattcgata tattatcgac acggcggatt cggcggggaa
     1201 attgttccaa gataaacaga tttccgctct gatctaaaac gacactatct gcctcagtag
     1261 cggcttcaag tgcagggcct ccatctcctc ttgaaccagg agcgccattt ccagcaaccg
     1321 tggtgattat gtttgtccgt gcatcgactc ggcgaattcg attgtttcct gaatcggcaa
     1381 taaataaatt tccatttgga tcaattgcaa cattcgttgg gagaaaaatg tctgcttgag
     1441 atgcaggtcc cccatcacca ctaaattcga ctataccggt tccgacaata gttttaataa
     1501 ttccagattc cgcatccact cggcggattc gctgattttc tctgtcagca ataaataaat
     1561 tttctgcttg atccactgca ataccaccgc ttttttggaa atcgctgaac aaacctccga
     1621 ctgacagact tgcagcctgg gctgggccat tatcaccgct aaatccacgc cttccatttc
     1681 cggcgacagt tgaaataatc cctgatttca ggtttatttg cctgactcgt tggttgccta
     1741 aatcaagaaa aaataaagtt cctttcgaag agagagccag actcagtgga tatgccagtc
     1801 gagctgccag ggctggacca ttgtctccat caaagccttg ccgccctgtt ccggcaatgg
     1861 ttgtaatcgt tcctgttttc gcatcaattc ggcggatgcg gtgactataa gtatcagcaa
     1921 taaataaatt cccaattgag tcagccacaa ttcgggtggg tcggttcagg atggcatttt
     1981 gttgattatc tccatcgcca aggtattcta ttccgccagc aattgtcgta acttctccat
     2041 ctgcaagttc gtctagtttt ttcggaatga tctgaaacgg catttgggca atcccatttg
     2101 gtgtccaaac cgaaagattc cgaactccag gtgctttttg gggcgggaca gtaaacttga
     2161 tcttttttcc tttgatgacc tgagtattgg tggccaccgc atctccaaca accacaaccg
     2221 tgtctggggt gaagtttttt cctttgatct caacctgagc cccaccttcc aatcggtcgg
     2281 aagaaagaga gattcttcgg attttcggtg tttgatcggt ttggatttgt gggattggag
     2341 ttgccttggc aactgtgctt tccccagttg ataggaaagg gttagcaaac gaactctctt
     2401 gagtaattaa tagcaaacca atgatggaac caattcttaa ataccaattc aacttggtct
     2461 ggttcatggt gtgtcctcac tttgatcaat gcccgacata ggttgaggtt ccctttggag
     2521 acagtcgaag aaatgaaatc aggccgttgt tttccaataa tacaaaagtt gagccgggac
     2581 tgacactgac gccatttggt aatttcaaat tgagattatc tacggtaccg atgatctttg
     2641 ctgggcatcg gacacctaaa tcatagattt tttttgttga tgttggagaa aaaccagcca
     2701 attgtagtct tgaatcagtc gaatcaggtt tgaatgttaa aaaagttgag gttgtgtcta
     2761 cagtagcttg aagaatcaca ttgggcacct ggtcaacacc gagatcatct cgaaagcgat
     2821 tcgtcgtgaa attacgatcc aggctgtagt agcttgtcaa tacaggttcc cacccaggat
     2881 tctcgaaatc agccgttgtt gttgggttaa ccttttcgaa gtttccagtt aagtatttca
     2941 gcacttccag gtgaccattt gcatagtttc ctggttttcc atcaattcct gcccaagcaa
     3001 gcacatataa atcttctaat ttcagaacat ctaaagcaac ggctttttca ttagctaata
     3061 ttccctgtgc cgtcacgtta ctgaccattt tccctgtatt cgcatccaaa accttaatcc
     3121 tcacaaaagg aatattatca atttcatctg gaagtgttgg attattgccc aacttggggt
     3181 gaggtgcatc attcacgaac atcaaagttg tcggtactcc attttcatca ttcccttcaa
     3241 taattccatt tactggggtt ccagcaacaa gcattgggtt agatccccgg tcctccttca
     3301 gattgttgaa gaatactgcc tttttggtga agaaatatct tgtgatattt gaccgattag
     3361 tggcatatcg gaaaaacagg tgattgcgtt gccgttcatc acgggcatat tgcccaagcg
     3421 tttgattttc acaa
//
LOCUS       DAOQDO010000404         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20690_length_3433_cov_2.762285, whole genome shotgun sequence.
ACCESSION   DAOQDO010000404 DAOQDO010000000
VERSION     DAOQDO010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20690_length_3433_cov_2.762285"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKZ53_07800"
     CDS             <1..911
                     /locus_tag="PKZ53_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40377.1"
                     /translation="SHQFADALVTTAIFLKRTVWPAGLSFFYECDDPIAWWHLLIAGV
                     VVFGFSVGSLWMIRRAPYLAVGWWWYLLVLFPVSGIVAIATPRADHYSYFSLIGIFLA
                     LSLGLADVVKQKPGLRFVAATGAGVLLLGLSGLSWNQAQTWKNSLTLFQHATLVDPNN
                     KIAHHKLAEEYFRQEQYELASQHFEAAGNEWGVALANQKNQAGQIQALRHQLAANPQD
                     ARTQLALGQTYAQRGQPEDALPYLQRSTELSPNEIEAWIALGKVYLQTGEHPKALDAF
                     SRALKLDPNRIEVRQQVETLREKTGK"
     gene            complement(1242..1514)
                     /locus_tag="PKZ53_07805"
     CDS             complement(1242..1514)
                     /locus_tag="PKZ53_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40378.1"
                     /translation="MRFHKQHLSVLLFGLIALVLLAGTQTLAASFGRYNVYCANGKLE
                     VDSRTLDQMKAARGNNVCTLAEFSNLADAESYASERGGKGSPCRCK"
     gene            complement(1736..2968)
                     /locus_tag="PKZ53_07810"
     CDS             complement(1736..2968)
                     /locus_tag="PKZ53_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ40379.1"
                     /translation="MSQVSLLYHVELVDVGGWLESFLLGLTFSVWNGFMTDSSSVELG
                     TFRGIHVRVSRAWAFIFLVVTLLLAERAASEFPAWTPGKRYSWGLCASLLFFLSVLIH
                     ELSRGLVAQANGIQVHSVTLQPFGGLARFAREALKPVEEFWIALTGPLVSLMTGAILL
                     TLALLTETQLKSFSFMLREIGAINILLGVFNLLPGFPLDGGRVLRSAIWWFTGDFRKA
                     TYVATRFGQGVAGLFLFVGIFLLLEKRGLGLLWVLFGLYLFGAAWNGYRQAELRERLR
                     AIHIQDVGWEELPFVPADQTATEFLDIHDFGARNRLFLVLNGEVIAGIITMELLRLLR
                     STLEEKDLQTVRLRDYMLPIERFERISPDTDVVVALEQMEFANVPCLPVVKDNELQGF
                     VSREAILEVIRQQFEDEL"
     gene            complement(2937..>3433)
                     /gene="nuoB"
                     /locus_tag="PKZ53_07815"
     CDS             complement(2937..>3433)
                     /gene="nuoB"
                     /locus_tag="PKZ53_07815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNJ40380.1"
                     /translation="PMTFGLACCAIEMMATGASRFDLDRFGAGVFRPSPRQSDLMIVA
                     GTVTLKMAPVVRRIYEQMPEPKWVISMGSCSNVGGPFNTYSTLQGVDRIVPVDVYVPG
                     CPPRPEALMYGLMRLQDKIMREHPTRFMFGRGDHFLELPDETEAVVTPAESSDVSSES
                     SVSR"
BASE COUNT          957 a          923 c          792 g          761 t
ORIGIN      
        1 tctcacatca atttgccgat gcgctggtta ccacggcgat tttcctcaag cgaactgtct
       61 ggccagccgg gttgtcattt ttctatgaat gcgatgaccc gattgcctgg tggcacctcc
      121 tgattgccgg agtggttgtt ttcggttttt cagttggttc gctctggatg atccgacggg
      181 ccccatatct ggcggttgga tggtggtggt atctcctggt tctgtttcca gtcagcggca
      241 ttgtcgcaat tgccacccct cgggctgatc attacagcta tttttcactg attgggatct
      301 tcctggcact cagtttgggg ctggcagacg tggtcaagca gaagccggga cttcgatttg
      361 ttgcagcaac tggcgccggt gtgcttctgc ttgggttgtc aggtctttcc tggaaccagg
      421 ctcaaacctg gaaaaacagc ctgacgttat ttcaacacgc aaccctggtt gatccgaata
      481 acaaaattgc ccatcacaaa ctggctgagg agtactttcg acaagaacaa tatgaactgg
      541 ccagccagca ttttgaagcc gctgggaatg agtggggagt agcgctggcc aaccaaaaga
      601 accaggccgg gcagattcag gcacttcgac accaactcgc agccaacccg caggatgcca
      661 ggactcaact ggccctgggg cagacatatg ctcagcgtgg acagccagaa gatgccctgc
      721 cctatttgca gcgatcaacc gaattgtcac caaatgagat tgaagcctgg attgcgttag
      781 gaaaggtgta ccttcaaaca ggtgagcacc caaaagccct ggatgctttt tcacgggcgc
      841 tcaagctaga tccaaatcgg attgaagtca gacagcaggt ggaaacgcta cgggaaaaaa
      901 ctggaaagtg aatcatacca atttgggctg agggtatcgg gctaagggct gaagaaaata
      961 caatattttc aacattttag cctttccata atgcgaggat ttcattccaa aatggtatca
     1021 caccagccca tcaagattga aagcgccgac aggtcggcgc actccaaaat tttctccaaa
     1081 acacaactgg cggcaataat gccgccagtt tctttttcga ttcaacaagt gagaggctat
     1141 atcactttac aatgaaacta tggtgtaaca gaaagactaa atcattgaaa ggattgtatt
     1201 tccctcagcc ctcagcccgc taccctcagc ccaaaatggt attatttgca ccggcaaggg
     1261 cttccttttc caccacgttc actggcataa ctttccgcat cggccaggtt actgaattca
     1321 gccagggtgc acacattatt gccgcgagcc gccttcattt gatccagcgt ccggctatca
     1381 acttcgagtt ttccatttgc acagtagacg ttatagcggc caaaactggc ggccagagtt
     1441 tgagtccctg ccaacaagac aagcgcaatg agtccaaaaa gcaataccga cagatgttgt
     1501 ttatgaaagc gcatatcgag catctccaat aataaggtcg aataaaaatc aaaatgaaat
     1561 aagcctgaac caggaagcga tccgatattt ggtgtgatgt cctggatttt tcaacggttg
     1621 ccttttcaaa cgagtcgcaa gtcgtcaatc aaaattcgtt tagaactagt tgaaggggca
     1681 ggtttgattt caatgggctt ttactcacta ctcacggctt gcgatcttgc tcatcttaaa
     1741 gctcatcctc aaattgctgg cggatgacct ccagaatggc ttcccggctc acaaatcctt
     1801 gtaattcatt gtctttgacg actggcaggc aaggaacgtt ggcaaattcc atttgttcga
     1861 gggccaccac cacatcagtg tcaggtgaaa tgcgctcaaa acgttcaatc ggcagcatat
     1921 aatcgcgcaa gcgaaccgtt tgcaggtctt tttcttcaag cgtagagcgc agtaaccgga
     1981 gcagctccat agtgataatt ccagcaatta cttctccatt taagaccagg aataatcggt
     2041 tgcgcgcgcc aaagtcgtgg atatccagaa attccgtcgc tgtctggtcg gctggcacaa
     2101 acgggagctc ttcccaacca acatcctgaa tgtgaatcgc ccgcagacgt tctctgagtt
     2161 cagcctgacg atacccattc caggccgccc caaacaaata gagtccaaat aaaacccaca
     2221 acaaccccaa accacgtttc tccaaaagta aaaaaatccc aacgaacaaa aacagcccgg
     2281 cgaccccctg gccaaagcgt gtcgccacat acgtggcttt gcgaaaatca cctgtaaacc
     2341 accagatggc tgaacgcaag actcgcccac cgtcaagtgg aaacccaggc agcaagttaa
     2401 atacacccag taaaatattg atggcaccga tttcacgcag catgaagcta aatgatttca
     2461 actgggtctc agtcagtaaa gccagcgtga gcaaaattgc cccagtcatc aggctgacca
     2521 gtgggccagt cagggcaatc caaaactcct caactggctt cagggcttcg cgggcaaacc
     2581 gggccaaacc gccaaagggt tggagcgtca cagaatgaac ctggattccg ttggcctgag
     2641 ccaccaatcc acggctcaat tcatgaatta agactgagag aaaaaacagt aaactggcac
     2701 acaaccccca gctatagcgt ttcccaggtg tccaggcggg aaattcagat gccgcccgct
     2761 ctgccagcaa aagagtcaca accagaaaaa tgaaggccca ggcacgactt acccggacat
     2821 gtatgccccg aaaggtgcca agctccacag atgaggaatc cgtcataaat ccattccaaa
     2881 cagaaaaagt caatcccagg agaaacgact caagccatcc tccaacatca accagctcaa
     2941 cgtgatacag aagactcact tgagacatca gatgattcgg caggtgtcac cacagcttcg
     3001 gtttcgtctg gtaattccag aaaatgatcg ccacggccaa acataaaccg ggtcggatgc
     3061 tcgcgcataa ttttatcctg caaccgcatt aacccataca tcagggcctc ggggcgtggt
     3121 ggacatccag gtacatacac atccaccggg acaattcgat ccaccccctg caatgtcgaa
     3181 taggtgttaa aggggccgcc gacattcgaa caagaaccca ttgaaatcac ccatttgggt
     3241 tccggcattt gttcataaat gcgccgaacc accggggcca tcttcaaggt gacagttcca
     3301 gccacaatca tcaaatctga ctggcgcggg ctaggtcgaa aaactccggc cccaaaccga
     3361 tccaggtcaa agcgtgaggc cccagtcgcc atcatttcaa tcgcacaaca ggccagacca
     3421 aatgtcattg gcc
//
LOCUS       DAOQDO010000405         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20706_length_3431_cov_2.832642, whole genome shotgun sequence.
ACCESSION   DAOQDO010000405 DAOQDO010000000
VERSION     DAOQDO010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20706_length_3431_cov_2.832642"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1810
                     /locus_tag="PKZ53_07820"
     CDS             425..1810
                     /locus_tag="PKZ53_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40381.1"
                     /translation="MTKRRKIILAVLVLLGIGLVTATGVFFAWLNGPGFDNWVKTQVI
                     DELKTCGIEAEFDSLDVHPLALKVDVRGMRLRLTGDIEPFMATDHITSQLLLRNLWTQ
                     DFRLQDLKIEHPVVRLKVDEQGKLNLARIKIPETPPRQGQAQPTPPAQFLSGIVALTG
                     GEIFFNEERYQLNTGLKNLRVTVSSQSPALKFDVGFSESQIQWQTKKIEAIELSTQLV
                     LDEHKADVKAFEIKTPYGETSLSTVVTWETPASPSTPAKLNYEGNLLSKLDLKRIATD
                     FLNQAPLGGQAQLTGKIIGDQNSYRIEGNLKETDLSTYGATFSALSLTYDFASPLQAF
                     LPELKSQIAFRGVTYDRYELKDFQAKVHLTPQFVDIEPFEARVLNGKVQGTSHIALGT
                     LLTADSTSVNSQSTTKLTFSDLDLQTALAMAALRQIPMTGKTTGTIELAWPELNVQQA
                     TGTQKPNWPEW"
     gene            1804..>3431
                     /locus_tag="PKZ53_07825"
     CDS             1804..>3431
                     /locus_tag="PKZ53_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40382.1"
                     /translation="MVTSISKPKAVTSDAAEPTEPASASDPNPPAVAQSRKPIPFQGD
                     LATKLLTGGVDIQKLALKLGDSTLDVKGLYQWNRQTKLDVHFQSPELAEAQELAERFG
                     VKTPQIPGSTGQVAVGVGGAGEFTGVVSGLLTGPTVSGTVSVAEVRVARSIVGKVSAE
                     FESTPQTLTVKQALFEQPQGGTVTVDIVGGLSGNQKTNLNVTIDQIRLKPIGEILAQI
                     YPPGSLEVGGKMDLTQALSDSEGRISGSMNLINLPPLLALAKGEFLTRSSNGTVSLPK
                     DISGKTELTADQLPSPYGTLESTRLLVSFEAGKAYRLDQFVSKLPFGALTATGAYDVV
                     QSQYTAEAHARDLDLTYFGEPLKAKGYPVTGIASLDLTSQGKFSDLLNGTPRFTLLAT
                     SPLVTVGKAPISNLKTRLEGEGNGAVAVTLASGFADQPYEASGKILLNLDTEDTGEFK
                     PTIDVVLNLKQTQLTPILAVFDAASPSFDGEATGSIRLIGPLFKMTQDVSGVEGVEFT
                     TEQVNLVGDFSELRFGVRATDDTDSAYEITNEGPV"
BASE COUNT          872 a          963 c          815 g          781 t
ORIGIN      
        1 ccaaacttca tgttaatcac gctggttgtc gtgagccggg atgtcaactg gatacacggc
       61 aacgcccttc ccgccttgat aaaccctgcg tcaagttgcg catcccgtaa aggctgcttc
      121 aacctgggct ggctggtttt tagattgacc tggagtgatt cgaaacttga tatggctcgg
      181 tgaacgccag tagtataggc tctaattgaa acccctgggt tgtctggctg tcaatgaaga
      241 ttcaacattc ggtttcaggt tcttggctcc tggcttttgg catatcaaat ctgggttcaa
      301 tttgaggatc tgaaatatca gtcgccagac cctcaaccca accaccagac aacgcttctg
      361 agtcccaatt caacagattt gtgcggtcca ccaattcgct tttcttttct caatcatcag
      421 ctttatgaca aaaagacgaa aaatcattct ggcagtcctg gtactgctcg gaattggttt
      481 ggttacagca actggtgttt tcttcgcctg gctcaatggt ccgggatttg acaactgggt
      541 caaaacccag gtgattgatg aacttaaaac ctgtggcatc gaggctgaat ttgactcact
      601 cgatgttcac cctctggcgc tgaaagttga tgtcagagga atgcggctcc ggttgacagg
      661 cgatatcgaa ccctttatgg caactgacca catcaccagt cagttgctgc tgcgcaatct
      721 ctggactcag gatttccgac ttcaggattt gaaaatcgaa cacccggtgg ttcgactcaa
      781 ggttgacgag caaggaaaac tcaacctggc ccggatcaaa attccggaaa ccccaccacg
      841 tcagggccag gcacagccaa ccccacccgc tcaatttctt tccggcatcg ttgccctcac
      901 cggtggcgag attttcttca atgaggagcg ctatcaactc aacaccggcc tcaaaaatct
      961 ccgtgtgacc gtcagttcac aatcaccagc actcaagttt gatgtgggtt tttcggaaag
     1021 ccagattcag tggcaaacca aaaaaatcga agcaattgaa ctctcaaccc aacttgtcct
     1081 ggacgagcac aaagcggatg tcaaagcctt tgaaatcaaa acgccctatg gcgaaaccag
     1141 tctgagtacc gtggtaacct gggaaacacc cgcctcccca tcaacacccg ccaaactcaa
     1201 ttatgaagga aacctgcttt ccaaacttga cctcaagcgg attgcaactg atttcctcaa
     1261 tcaggcgccg ctgggtggcc aggcccagct caccggcaaa atcatcggtg accaaaactc
     1321 ataccgcatt gaaggcaatc tcaaggaaac ggatctttcg acctatggtg ccacattttc
     1381 agccttgagt ctgacctatg actttgcctc acccctccag gcatttctcc ctgaattaaa
     1441 gagccagatt gccttccgag gggtcaccta tgatcgctat gaactcaagg acttccaggc
     1501 aaaggttcac ctgactcccc aatttgttga tattgaaccg tttgaagcca gagtcctcaa
     1561 tgggaaagtt caaggaacct cacacattgc gctgggcacc ttgctgactg ccgactcgac
     1621 atcggtcaat tcccagagca caaccaaact tacgttttca gacctggacc tgcagacggc
     1681 tctggcaatg gcggcgctgc ggcaaattcc gatgaccgga aaaaccaccg gtaccattga
     1741 actggcctgg ccggaactca atgtgcaaca agcgacgggc acccagaagc caaactggcc
     1801 ggagtggtaa ccagtatcag caaaccaaaa gccgttactt cagatgcagc cgagccaacc
     1861 gagccagcat ctgcgtctga tccaaaccca ccagccgtcg cccagagccg gaaacccatt
     1921 ccattccagg gggatttagc caccaaatta ctcactgggg gcgttgacat ccaaaaactg
     1981 gccctcaaat tgggcgacag cacgctggat gtcaaaggtc tgtatcaatg gaatcgccag
     2041 acgaaactgg atgttcattt tcagtcacca gaactggctg aagctcagga actggccgaa
     2101 cgatttggcg tcaaaacacc ccaaatccca ggctcgaccg gacaggttgc cgttggcgtt
     2161 ggcggcgccg gcgaatttac cggtgtggtt tctggtttgt taactggacc aaccgtgtct
     2221 gggacagtca gcgttgctga agttcgagtg gcgcgatcca tcgtgggtaa ggtttcggct
     2281 gaatttgaat ctaccccgca aaccctgacc gtcaaacagg ccctttttga acagccccag
     2341 ggcggcaccg tgacagtgga cattgtcggc gggctttccg gcaatcagaa gaccaacctc
     2401 aacgtcacca ttgaccagat ccgactcaaa cccattggcg agatcctggc ccagatttat
     2461 ccgcctgggt cactcgaagt aggcgggaag atggatctca ctcaggcatt aagtgattcg
     2521 gaaggccgca tttcagggtc aatgaatctc atcaacctgc caccactgct ggcactggcc
     2581 aaaggtgagt ttttgaccag atcctcaaat ggcacggttt cactcccaaa ggacatcagc
     2641 ggcaaaaccg agcttaccgc cgatcagctc ccatcgccct atggcacact tgaatcaacc
     2701 agactgctcg tttcctttga agccgggaaa gcctatcgcc ttgatcaatt tgtgtcgaag
     2761 cttccatttg gagccctgac tgccaccggt gcctatgatg tggtgcaatc tcagtacacg
     2821 gctgaggccc acgccagaga tctggacctg acctattttg gcgagccact caaagccaaa
     2881 ggatatccag tgactggcat cgccagcctg gatctgacca gccaggggaa attttctgat
     2941 ttgttaaatg gcacgccccg ctttaccctg ctcgccacca gcccgctggt aaccgttggc
     3001 aaagcaccaa tttccaactt aaaaacacga ctggaaggcg aaggcaatgg agcagtggca
     3061 gtaacactgg cttccggctt tgccgatcaa ccctatgaag ccagcggaaa aatcctgctc
     3121 aaccttgata ctgaagatac tggcgaattt aagcccacga tagatgtcgt cttaaacttg
     3181 aaacagaccc agttgacccc aatcctggcg gtttttgatg cagcttcccc ttcctttgac
     3241 ggcgaggcca cgggttccat ccggttgatt ggcccattat ttaaaatgac tcaggacgtc
     3301 tctggggttg aaggagtcga gtttacaacc gaacaagtca atctggtcgg cgatttttca
     3361 gagcttcgat ttggcgttcg tgccaccgat gacaccgata gcgcctatga aatcaccaac
     3421 gaaggcccgg t
//
LOCUS       DAOQDO010000406         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20746_length_3428_cov_2.701156, whole genome shotgun sequence.
ACCESSION   DAOQDO010000406 DAOQDO010000000
VERSION     DAOQDO010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20746_length_3428_cov_2.701156"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /gene="dnaK"
                     /locus_tag="PKZ53_07830"
     CDS             complement(<1..1347)
                     /gene="dnaK"
                     /locus_tag="PKZ53_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNJ40383.1"
                     /translation="MGKVIGIDLGTTNCCVAVMEGGTTQIIPNKEGGRTTPSVVGFTD
                     KGERLVGQIAKRQAITNAANTVYAVKRLIGRKFNSPEVQRARESSPYEISEAPNGDAR
                     VRVQGRDYSPPEISAIILQRLKASAEDFLGEEIEEAIITVPAYFDDFQRQATKDAGKI
                     AGLNVQRIINEPTAAALAYGLGKTQSERISVYDLGGGTFDISILEMNDGVFEVLSTCG
                     DSFLGGEDFDQRIIDWLIEEFKKQTAIDLRADRLALQRLKEAAERAKCEVSTSLEANI
                     NLPFIAADATGPKHLNINLTRAKFEELVSDLVEKTVEPCQKALWDARLKPEDIDQVLL
                     VGGQTRSPIIHECVRKIFNKDASTAINPDEVVAIGASIQGAVLTGEVKELVLLDVIPL
                     SLGIEVRGGLFQKVVERNSTIPIRKGLTFTTVADNQNSVEVHILQGEREIVAGNRSL"
     gene            complement(1623..2471)
                     /gene="grpE"
                     /locus_tag="PKZ53_07835"
     CDS             complement(1623..2471)
                     /gene="grpE"
                     /locus_tag="PKZ53_07835"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNJ40384.1"
                     /translation="MTNIPVHFESSNQKEEPAPQTPETRQPESGAIEAEPQPEAVAAQ
                     PAEVPPAVASTDVAEPEPAPVPEPASKAVDLVEAPPEPPVEAKPQSSGGLTMEETVQL
                     LTDLAMLQENLAHAEQQTLLARKHVEIVQEQLAKVNEEKNSWHEQLLRQRAEFENFRK
                     RVEREKQELRLQSRADVIRSTLEVLDNLERALSIGQSDSGNLEAFLEGVQLIQKQLFG
                     SLTNFGLTPVKAVGEMFDPSIHEAVATQVSEELEPNTIVDELQRGYLLGDRLIRPARV
                     RVAIRG"
     gene            complement(3001..>3428)
                     /locus_tag="PKZ53_07840"
     CDS             complement(3001..>3428)
                     /locus_tag="PKZ53_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNJ40385.1"
                     /translation="VDGTVNMLNQPDFVDLDRMRALFRMFEEKGRLVKILNKCLSMVP
                     DGGIAIQIGAENQLPGMRDCTIITSPYSFSNQVIGGISIVGPTRMHYTRVISIVSYVS
                     QLFNQLLNEEGPFEIRPDGSLHLIDPKVKPAQFALPVPE"
BASE COUNT          839 a          880 c          864 g          845 t
ORIGIN      
        1 caacgaccgg ttgccagcca cgatttcacg ctctccttgc aaaatatgga cttcaactga
       61 gttctggtta tcggctacgg tggtaaaggt caggcctttg cggattggaa tcgttgagtt
      121 gcgttcgaca actttctgga acaagccacc ccggacttca atccccagtg agagcggaat
      181 cacgtcaagc aggaccagtt ctttaacttc accggtgagg accgcacctt gaatcgaagc
      241 gccaatcgcc accacttcat ccgggttgat ggcggttgaa gcatctttat taaaaatttt
      301 gcggacacat tcgtgaataa ttggggagcg agtttgccca cccaccagca gtacctgatc
      361 aatatcttcg ggtttgagac gggcatccca gagggctttc tggcagggtt cgactgtctt
      421 ttcaaccaga tcagagacca gttcttcaaa cttggcccgg gtcagattga tatttaagtg
      481 tttagggccg gtcgcatcag ccgcaataaa cggcaggttg atattggctt ccagcgaggt
      541 ggaaacttca catttggccc gttcggcggc ctctttcaat cgctgcaggg ccagccgatc
      601 tgcccgcagg tcaatggcgg tctgcttttt aaattcttcg atcaaccagt caatgatccg
      661 ctggtcaaaa tcttccccac ccaggaacga atcgccacag gtggaaagaa cttcgaagac
      721 gccatcgttc atttccaaaa tggagatatc aaacgttcct ccacccaggt catagactga
      781 aattcgttcg gattgggttt tgcccagccc ataagccagc gccgccgccg tcggttcgtt
      841 gatgatacgc tggacattga gcccggcaat ttttccggcg tccttggtcg cctgacgttg
      901 aaagtcatcg aagtaggccg ggacagtgat gatggcttct tcgatttctt cgccgagaaa
      961 atcttcagcc gatgccttga gtcgttgcaa gatgatggct gaaatttcag gtggactata
     1021 gtcacgtccc tggacacgga cccgggcatc gccattggga gcttcagaga tttcgtacgg
     1081 actgctttcc cgtgcccgct ggacttcagg ggagttaaat ttgcgcccaa tcaaccgttt
     1141 cacggcatag acagtattgg ctgcatttgt aattgcctgg cgcttggcaa tctgccctac
     1201 gagtcgttcc cccttgtctg taaacccgac gacagatggt gttgttcggc caccttcttt
     1261 attgggaatg atttgcgtcg ttcccccttc catgacagcc acacaacagt tcgtcgtacc
     1321 caagtcaatg ccaatgacct tccccataaa cttttcagcc tttcctaaag acttactgaa
     1381 tccccccgac tgagtacggg agtgaaggag tgttttcggt taaatagatc catctggcag
     1441 ttttttgttt ttgccacatt gacaaacagc aaagcgccgc caattggtgg gtgaacattc
     1501 attaaaaatc agtcaacaca aaagctggct gccaaaaggc caaaaaccag tggccgccat
     1561 tgccaatcgc cgcaccaaac cagaggagtt ccctttggtg ttcagggtca cgcccgatca
     1621 ggttagcctc gaatcgcaac acgaactcgc gcaggacgga ttaagcgatc cccaagtaag
     1681 taaccgcgct ggagttcatc aacaatggtg tttggttcaa gctcttctga cacctgagtg
     1741 gcgaccgctt cgtggatcga aggatcaaac atttcgccaa ccgctttgac tggcgtcaaa
     1801 ccaaagttgg tcaaggagcc aaacaactgt ttctgaatga gttggacccc ttcgagaaaa
     1861 gcttctagat tcccggaatc tgattggcca atactgagtg cccgctccag attatcgagc
     1921 acctccaggg tactgcggat cacatcggct ctggattgca gtcgcagttc ctgtttttca
     1981 cgttcaaccc gcttgcggaa attctcaaat tcagcgcgct gacgcaggag ctgttcgtgc
     2041 catgaatttt tttcctcatt gacctttgcc agttgctcct gaacgatttc aacgtgtttg
     2101 cgagccagca gtgtttgctg ttcggcatgg gccaggtttt cctgcaacat cgccagatca
     2161 gtcagaagtt gaacggtttc ctccatggtg agaccaccgc ttgactgtgg cttggcctca
     2221 actggcggtt ctggcggagc ttcaaccagg tcaaccgctt tggaggcagg ttccggtact
     2281 ggcgccggtt ccggctcggc cacatcggtc gaggcgactg ccggtggaac ttcagcaggt
     2341 tgcgccgcaa ccgcctctgg ctggggttcg gcttcgatgg caccagattc aggctgtcgt
     2401 gtttccggcg tctggggcgc gggttcttct ttttggtttg atgactcgaa atgaacggga
     2461 atattcgtca cagaccctcc gggtggttag tggctcaatg tcagaaatga aatgaacaca
     2521 ggataacagg taaagatatt ttgccgctct gtcattcttt tttcccagtt tcgagcggct
     2581 gatatcagac agtaccgcaa aaaaccaaaa ccagtcagaa ttgatacatc aagggcaaag
     2641 gcaactgttt tggatgaaca atggccgttt gcggtggatg aaacagcaaa tgattacaaa
     2701 aacgcggtat ggaattggtc agttgcggcc caggacagtc gactgaagac actaaaacaa
     2761 tgaaaagagc gttacagcaa acaaaaattg accggtgatg gactcaccaa tggatgcggg
     2821 cagtgatgga gaaacactgg ttttggaaaa ctgtgtgctt ccctacctta ccgaaaacca
     2881 ggcaatgttg ccaagcggcg gcgtgacaga tccacgaaaa aaaccagcct gtgaaccaaa
     2941 tttcctctgg gtgtcagcac cctggaagcg atgtggcgtg agaaaaacac tggccacaac
     3001 ttactctgga acggggaggg caaattgagc tggttttact ttcggatcaa tcaggtgaag
     3061 tgaaccatcc gggcgaattt caaagggtcc ctcttcattg agcaactggt taaagagttg
     3121 ggaaacatag ctcacaatgc tgatgactcg ggtgtaatgc atccgggtcg gccccacaat
     3181 actaattccg ccaatgacct ggttactgaa tgaatagggc gaggtgatga tggtgcaatc
     3241 acgcatacct gggagctgat tttcagcacc gatttggatg gcaatcccgc catcgggcac
     3301 cattgacagg cacttgttca aaattttcac cagccgccct ttttcttcaa acatccggaa
     3361 caaagctcgc atgcgatcaa gatcaacaaa atcaggctga ttcaacatat tgaccgttcc
     3421 atcaacat
//
LOCUS       DAOQDO010000407         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20752_length_3428_cov_2.418618, whole genome shotgun sequence.
ACCESSION   DAOQDO010000407 DAOQDO010000000
VERSION     DAOQDO010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20752_length_3428_cov_2.418618"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKZ53_07845"
     CDS             <1..1066
                     /locus_tag="PKZ53_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNJ40386.1"
                     /translation="EEQTQVTARQVQDALTGNLCRCTGYEPIITSALKVETERLTRFQ
                     ARYPTEEMLATLAQHQEPVHFEFKNKVFYSPVDLAGAVAFKAEHPQTLIVQGATDVGV
                     WCNKRGYVTQTVMSLNQVSELKTLTVEDGLLIVGARVTLQDLEDFTRDVVPEFHRILS
                     IFGSPQIKHAGTLAGNIANGSPIADSLPFLFVTQAQVELTGLNGSRLVDINQLYLGYK
                     QLDIQPDELITRVLIPLPAAGEILKLYKVSKRKDLDISSFTAGFKLALTGDIIESIHI
                     AYGGVGPVVLRLPKTEAFLTGRELSFEAIEQAGQLARQEISPISDVRGSSQFRFQLAE
                     NILKKFYFELQEERESVCRS"
     gene            1054..3390
                     /gene="xdhB"
                     /locus_tag="PKZ53_07850"
     CDS             1054..3390
                     /gene="xdhB"
                     /locus_tag="PKZ53_07850"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNJ40387.1"
                     /translation="MSVVGKNIPHDSAAGHVSGESIYIDDMPFSRQEVLVDFYWSPVA
                     HGKINSIDISQACLIPGVVGVYTAKDLGGVNKFGPIIKDEPLLAETEVLFIGHPIAVI
                     AAETRDAIAAAKAAIKVEIEPLEPVFTIDEAIAREWFLGQKRTIRRGNVAAALAEADH
                     VLEGTFVCGGQDHFYLESQAAIVYPGEHNTLTVHSSTQHPTEVQEIVAKVLGLQFHQV
                     VCITKRMGGAFGGKESQATHPACMAGLVALKTRRPARIVFNKDDDMMVTGKRHPFQNR
                     YQVGFHRDGRITAFKVEIYSNGGAYADLSTAVMARAMTHVDNAYFLPNVEITGVVCRT
                     NLPPNTAFRGFGGPQGAATIEAVMEEIGIFLKKDPVDIRLANLYGITDRNTTPYGQLV
                     RNNLLPRLFDEQLTRSDYRRRRAEVEAFNVSSKTHLKGISCTGVKFGISFNTKFLNQA
                     SALVHIYLDGSIQVSTGATEMGQGVNTKIKQLVADEFSIDPHWVITMPTSTEKSNNAS
                     ATAASSGADLNGGAAVDACQRLRARLTEAASEVLASREPDLEKSPANICFENGFIYDN
                     RRPSQCISFKDLVKYAYHQRISLGERGFYATQGVDFNWETGSGHPFLYFTMACAVSEV
                     LIDRFTGDLQVLRSDVLMDIGKSINPGIDRGQITGGFIQGLGWLTSEELKYNAKGELL
                     SHSPTTYKIPNIQDVPAIFNVDWIDNDTNDVNVRRSKAVGEPPFLTAISVWTAVKHAL
                     SCVSNGEIPVLHVPATNEEILTRLTDLARRPLELRMKN"
BASE COUNT          826 a          870 c          879 g          853 t
ORIGIN      
        1 tgaagagcaa acacaggtga ctgcccggca ggtccaggat gcgctcaccg gaaacctctg
       61 ccgctgcact ggctatgaac ccattattac ctcagccctg aaggttgaaa ctgagcggct
      121 gacccggttt caagcccgat atccaactga ggaaatgctg gcgactctgg ctcaacacca
      181 ggaaccagtt cactttgaat tcaaaaataa ggtgttttat agcccggtgg accttgccgg
      241 ggctgttgcc tttaaggccg aacatccaca aaccttgatt gtccaggggg cgacagacgt
      301 tggtgtgtgg tgtaacaagc gaggctatgt cacccagacg gtgatgagtc tcaatcaagt
      361 ctcagaactc aaaacactga cggttgaaga tggcctgttg atagtcggcg cccgcgtgac
      421 cctgcaggat ctggaagatt tcacgcgtga tgtggtcccg gaatttcatc gcattttatc
      481 tatttttggt tctccacaga tcaaacatgc tggcaccctg gccggcaata ttgccaatgg
      541 ctcgccaatc gctgattcac tgccattttt atttgtcacc caggcccagg ttgaattgac
      601 tggactcaat ggctcacggc tggttgacat caaccagttg tatctcgggt ataagcaact
      661 tgatattcag ccagacgagc tgattacccg cgttctgatc ccattgccgg cggctgggga
      721 aatcctcaaa ctctataagg tttcaaagcg caaggatctt gatatttcat cctttacggc
      781 tggattcaaa ctggccctca cgggtgacat cattgagtcc attcacattg catatggtgg
      841 tgttgggccg gtcgtgttgc ggcttcccaa aaccgaagct tttttgaccg gtcgtgagtt
      901 gtcctttgaa gccattgaac aggccggaca actggctcga caggaaatca gcccgatttc
      961 agatgttcga gggtccagtc aatttcgctt tcaactggcg gaaaacattc tcaagaaatt
     1021 ttactttgaa ctgcaggaag aaagggaatc ggtatgtcgg tcgtaggcaa aaacatcccg
     1081 cacgattcag ccgccggaca cgtttccggt gaatcaattt acattgatga catgccgttt
     1141 tctcgccagg aggtactggt tgatttttac tggagcccgg tggctcacgg gaaaatcaat
     1201 tcgattgata tcagccaggc ttgcctgatc cccggtgtgg tgggggtgta caccgcgaaa
     1261 gatctgggcg gagtcaataa attcgggccg attatcaaag atgaaccgct gctggctgaa
     1321 accgaagtcc tctttattgg tcaccctatc gcagtcattg cggctgaaac ccgtgatgcc
     1381 atcgctgcgg caaaggccgc catcaaggtt gagattgaac ccctggaacc agtgttcacc
     1441 attgacgaag ccattgccag agagtggttt ctggggcaaa agcgcaccat tcgtcgtgga
     1501 aacgtcgcgg cggcactggc cgaagccgac catgtgctgg aaggaacctt tgtctgtggc
     1561 ggtcaggatc atttttacct cgaatctcaa gcggccattg tctatcccgg cgaacacaac
     1621 acgctgaccg tgcattcttc aacccagcat ccgaccgaag tccaggaaat tgtggccaaa
     1681 gtgcttggtc tgcaatttca tcaggtggtg tgtatcacaa agcgcatggg aggcgctttt
     1741 ggtggaaagg aatcccaggc cacccatcca gcctgtatgg cgggcctggt tgcgctcaag
     1801 accagacgcc cggcccggat cgtctttaat aaagatgacg atatgatggt caccggcaag
     1861 cgccacccct ttcaaaatcg ctatcaggtt gggtttcatc gggatggtcg cattacagcc
     1921 ttcaaagtcg aaatctattc aaatggcggg gcctatgccg atctttcaac cgcagtgatg
     1981 gcccgggcca tgacccacgt tgacaatgcc tatttcctgc ccaacgttga aatcactggc
     2041 gttgtctgcc gaacgaatct gcccccaaac actgcctttc gtgggtttgg cggtccgcaa
     2101 ggcgccgcca ccattgaagc tgtgatggaa gaaatcggga ttttcctgaa aaaagacccg
     2161 gttgatattc ggctggccaa tctctatggc attactgacc gcaatacaac gccgtacggc
     2221 cagcttgtca gaaacaacct gctccctcgg ctgtttgatg aacagttgac gcgctcagac
     2281 tatcgtcgcc gtcgggctga ggtcgaagcc tttaatgtca gttccaaaac ccatttgaaa
     2341 ggtatttcct gtaccggagt caaatttggg atctcgttta ataccaagtt tttgaatcag
     2401 gccagtgcgc tggtccacat ttatctcgat ggctcgattc aggtttcaac tggggcaact
     2461 gaaatggggc agggtgtcaa caccaagatc aaacaactgg tggccgacga gttttcaatt
     2521 gatccacact gggtcatcac catgccgact tcaaccgaaa aaagcaataa tgcctcggcg
     2581 acagcggctt ccagtggtgc tgatttaaat ggtggtgccg cggtggatgc ctgtcagcga
     2641 cttcgtgccc gactgaccga agctgccagt gaagtcctgg cgtcacgtga accagacctg
     2701 gaaaaatcac cagccaatat ttgctttgaa aacgggttca tttacgataa ccggcgacct
     2761 tcccagtgca tctcctttaa agatctggtc aagtatgcct atcatcagcg aatcagcctc
     2821 ggtgagcgcg gcttttacgc cactcaaggg gtagatttca actgggaaac cggctctggc
     2881 catccgtttc tttacttcac catggcttgt gctgtttctg aagtgctcat tgaccggttc
     2941 acgggggatt tgcaagtgtt gcgaagtgat gtgttgatgg atatcggcaa gtcaatcaac
     3001 cctggaattg atcgtggtca gattacaggt ggttttatcc agggccttgg ctggctcacc
     3061 tctgaagagt taaaatacaa tgccaaaggt gaactgctat ctcattcacc cacgacatac
     3121 aaaattccaa atatccagga tgtcccggcc atttttaatg tggattggat tgacaacgat
     3181 accaacgatg tcaatgtgcg acggagcaaa gccgtgggtg aacccccatt tttgacggca
     3241 atttcggttt ggacggctgt caaacatgcc ctttcctgtg tctcaaatgg cgaaatcccg
     3301 gttcttcacg ttccagccac caatgaagaa attttaaccc gattgaccga tctggcccga
     3361 cgtccgttgg aattgagaat gaagaattga gaatgaagaa tgtgcttagt gcttagtgct
     3421 tagtgctt
//
LOCUS       DAOQDO010000408         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20768_length_3426_cov_3.138238, whole genome shotgun sequence.
ACCESSION   DAOQDO010000408 DAOQDO010000000
VERSION     DAOQDO010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20768_length_3426_cov_3.138238"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKZ53_07855"
     CDS             <1..289
                     /locus_tag="PKZ53_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNJ40388.1"
                     /translation="YELGEIIYKYPFTTPTAFTFIMRALMTLEGISIQMNPEFNFLEV
                     ARPYARDFLFRRDSAKLREKVWQSLHDVRSGKFDWHRLYTLAKSAVSLYFA"
     gene            459..1472
                     /locus_tag="PKZ53_07860"
     CDS             459..1472
                     /locus_tag="PKZ53_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ40389.1"
                     /translation="MINQIKLKNFGPLSQIEWTNLGQINLVIGVNGSGKTFLLKALYS
                     AMRTLEEYKRGDVQETAAEILWRKLYWTFQPEKIGDLVTKGADSQLEFRLEFDQKEFA
                     YSFGKDTAKHISTLENHVPPRANNSIFLPAKEVLSLHHIILKSRDVDQTFGFDDTYLD
                     LARALTQSSKPGKNFTEFAKSKQSLETILGGRVEREETSGRWQFKKGNQRFPIGVTAE
                     GVKKIAILDTLLSNRYLAPQSIIFIDELESALHPIAISQLLEIVAMLAERGIQFFLAT
                     HSYFVVKKLFLIALEKNIPIPVISSDGESWKYANLKEGMPDNPIISQSIHLYEQEMEL
                     ALR"
     gene            1634..2008
                     /locus_tag="PKZ53_07865"
     CDS             1634..2008
                     /locus_tag="PKZ53_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40390.1"
                     /translation="MVIEAKSSSPRPETEPNFTQYIDEIREKLTNGLVLTIASILRRH
                     EVAQRELSQEFKDLDLATCEFRLILVINGHKKEWLPPLNDALTLSLHSTIKTWALGAT
                     SVMVLNEEMASKLGLTCTPSSK"
     gene            complement(2093..>3426)
                     /locus_tag="PKZ53_07870"
     CDS             complement(2093..>3426)
                     /locus_tag="PKZ53_07870"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ40391.1"
                     /translation="ATPENLTKASTFLKSRSGGGGTEMMKAIRAALEPSDSQNHLRVV
                     CFMTDGYVGDDLEILSEVKKHPNARVFSFGIGSSVNRFLLEEMARLGRGEVEFVGLED
                     DGSAAAQRFHERVRSPLLTDISIDWNGLPVKEVYPAQIPDLFSATPVVVTGRFEHSAR
                     GEVVLRGKVAGRPVEQRIQVELPAAEPQHDELATLWARQQLRELMRENYDGFQQGTVS
                     PELEARITRLGLKYRLVTPFTSFLAIDRGLTQRGKLKKERILAAAPDKGDDDGPVGPE
                     GPATGLGGFGDGDGDFGDREASGAVASPVFVPPREAPKEIVNTPPPAKIIFSDPNVLP
                     MPAPPPPPGRQPAPPSVVRRSEGVMRGNALARVTPEYPKAARSARVAGDVVVELVIDE
                     KGNVESVRVVSGHALLRQAAINAAQQWKFKPTLLNKAPVKVTGILTFRFTL"
BASE COUNT          919 a          865 c          771 g          871 t
ORIGIN      
        1 ttacgaactc ggcgaaatca tttacaaata cccatttacc actccgacgg cgtttacctt
       61 cattatgcgg gcgctgatga cactcgaagg catcagcatc cagatgaacc cggaatttaa
      121 cttcctggaa gttgcccgac cctatgcgcg tgattttctc ttccggcgtg acagcgccaa
      181 attacgcgaa aaagtgtggc agagtctgca cgatgtgcgc tccggcaagt ttgactggca
      241 ccggctctac acactggcca aaagcgcggt gtcgctttat tttgcataga aatatacggc
      301 ctgggttgat tctgatctat ctcttcagac tcaaagctcc gctgcagcaa cggcattttc
      361 ataaatgacg agaaacatct aaatctgtta atcttcaagc gacttctcag caggtagtgc
      421 tttaaattca aaggccatat caatctggag caagctacat gatcaaccaa atcaaattaa
      481 agaactttgg tccgctttcc caaattgagt ggacaaatct cggtcagatt aacctggtga
      541 taggggttaa tggaagtggt aaaacgttct tgctgaaagc actttacagt gccatgcgta
      601 ctcttgaaga atacaaacgg ggagatgttc aagaaacagc agcagaaatt ttatggagaa
      661 agctctactg gacatttcaa cctgagaaaa tcggtgattt ggtcaccaaa ggagccgatt
      721 ctcaattaga gtttcgtctt gaatttgatc aaaaggagtt tgcttattct ttcgggaaag
      781 acactgccaa acatatttcc acacttgaaa accatgtccc cccacgagcc aacaactcca
      841 tttttctacc agcaaaggaa gtgctttccc ttcatcacat tattttaaaa tcacgtgatg
      901 tggatcaaac atttggcttt gatgatacct accttgatct tgctcgggca ctcacccagt
      961 cttcaaaacc tggaaaaaac tttacggaat ttgccaaatc gaagcaaagc ctggagacca
     1021 tactgggtgg cagagtggaa agagaagaaa cttcaggccg gtggcagttt aaaaaaggca
     1081 atcagagatt tccgattggt gttaccgctg aaggggtgaa gaaaatcgca attctcgata
     1141 cgttgctcag taaccgttac ctcgctcccc aatcaatcat ttttattgat gaattggaat
     1201 cagcccttca cccaatcgct atttcccaac tccttgaaat tgtggcgatg cttgccgaac
     1261 gaggcattca attttttctc gcaacacact cttatttcgt ggttaagaag ttatttctga
     1321 ttgcattaga aaaaaatata ccaatcccag tgatttcatc tgatggtgaa agctggaaat
     1381 atgcaaattt aaaagaaggg atgccggata atccaattat cagccagtca atccatctgt
     1441 atgaacaaga aatggaactc gcgctgagat gactatggtt accatcactg aatcggggtt
     1501 gatttttggt ccataccctg aaaatgaatg cttcctggtt gaaaaaagtg caacctacca
     1561 gaacattcaa agtggagtaa aaatggctga attccttcgc cttcaacgaa gggattcgaa
     1621 gccacccctg atattggtca ttgaagcaaa gtccagttca ccacgccctg agacagagcc
     1681 aaatttcact caatatattg atgagatccg cgaaaagctg accaatgggt tggtgttgac
     1741 gattgcctca attttgaggc ggcacgaagt tgctcaaaga gaactctctc aagaatttaa
     1801 agacctggat ttagcaacct gtgaattccg gctgatactg gttatcaatg gtcacaagaa
     1861 agaatggctt ccaccgttaa atgatgcgct gactctatcg ctgcactcga caattaagac
     1921 gtgggcattg ggagcaacct cagttatggt tttgaatgaa gagatggctt ctaaacttgg
     1981 attaacctgt actccctcct caaagtaatc tgtctttcgg ctgactcaat tgtactgtca
     2041 acatagtttg agtggatgac tcgcctgacg agtcatccac tctgatgacc gactaaaggg
     2101 taaatcgaaa cgtcaggatt cccgtgactt tgacaggagc tttattgagc aacgtgggct
     2161 taaatttcca ctgctgggca gcattgatag ccgcctgtcg cagcaaggca tggccggaaa
     2221 caacacgcac gctttcgaca ttcccttttt cgtcaatcac aagttcaacc accacatccc
     2281 ccgcaacccg tgctgaacga gcagctttcg ggtattcagg tgtgacccga gcgagtgcat
     2341 tccctcgcat aacaccctcg cttcgtctga caactgaagg tggggcaggt tgccgacctg
     2401 gtggcggtgg gggcgcgggc ataggcaaca catttgggtc agaaaaaatt atcttcgcag
     2461 gtggaggggt gttcacaatt tcttttgggg cctccctcgg tggaacaaac actggggaag
     2521 caactgctcc agaagcttcc ctatccccaa aatctccatc gccatctcca aatcctccca
     2581 aaccagtcgc cggcccttca ggaccgacag gtccgtcgtc gtccccttta tccggggctg
     2641 ccgccaaaat ccgttccttt ttcagtttgc cacgttgagt cagaccacga tcaatcgcca
     2701 gaaatgaagt aaacggcgtc accagtcgat acttcagacc aagtctggtg attctggctt
     2761 ctaactcagg cgatacagtt ccctgctgaa aaccgtcata gttctcgcgc atcaactctc
     2821 ggagttgctg ccgggcccat aacgttgcga gttcgtcgtg ctggggttcg gcggcgggta
     2881 attccacctg gattcgctgc tcgactggcc gccccgcgac cttcccacgg agcacaactt
     2941 cgcctcgggc actgtgctca aatctcccgg tgacaaccac tggtgttgcg ctaaataaat
     3001 cgggaatctg tgccggatag acctctttga ccggtaaccc attccaatca attgaaatat
     3061 cagtcagcaa cggcgaccgg acccgctcgt gaaatcgctg tgctgccgcc gagccatcat
     3121 cttcaagtcc tacaaattcg acttctccac gaccaaggcg cgccatttct tcgagtaaaa
     3181 accgattgac tgagcttccg attccaaatg aaaacacacg ggcattcgga tgttttttga
     3241 cctcgctgag gatttccaga tcatctccga catagccatc ggtcataaaa cacacgactc
     3301 gaaggtggtt ttgcgaatcc gacggctcaa gtgccgcacg aatcgctttc atcatttctg
     3361 ttccgccgcc gccagaccgg gacttgagaa acgtgctggc ttttgtcaaa ttttcgggcg
     3421 ttgcgg
//
LOCUS       DAOQDO010000409         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20775_length_3425_cov_4.042730, whole genome shotgun sequence.
ACCESSION   DAOQDO010000409 DAOQDO010000000
VERSION     DAOQDO010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20775_length_3425_cov_4.042730"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..2827
                     /locus_tag="PKZ53_07875"
     CDS             149..2827
                     /locus_tag="PKZ53_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNJ40392.1"
                     /translation="MPVNSIFTRSELDDSRKKKPAMVQRFFRKSTFFAVLLLSALAGS
                     LAGLAVAYQSSLAGQDWGVAELASYQPWGITQVYASDGKSVIGEFALERRIPLSYDEI
                     PLHMRQAILAIEDARFEKHLGVDPIGISRALVKNTLAGRTVEGGSTLTQQLSKMLFLT
                     DERTYTRKIKEAILSLQIEREFSKQQIFELYANQINLGGGAYGVEAGAQYYFGKSAKD
                     LTIEECATLAAIPKAPSNYSPALNPEKSLNRRNLVINNMIEEGFISAAEGEAAKNKPI
                     KLNLTPRETNTSGPYAFFVEEVRRELENKFGSRETHSLGYKVITTVDAEAQVRAVQAV
                     RAGLHAYAQRHGAKWHDDLDNIFDQGVKDINKYKHPEWSLTLAEGMYVYGLITEINSR
                     EAKVSFGDYHAVVTEKEIAIAGGSPSKVFKVGDLAVFQIKKMNQPEKDKEAEKKEAKP
                     KTESTGDKDAKKKPKAEPQGDPFELSVELQVVPKVTGALLCIEAKTGEVTAMVGGYDF
                     SISRFNNATQGNRQTGSAFKPFIYATAVENGWKPESLVLDTPFTQGNWSPRNYDGSFS
                     GAIPLSTALAKSRNIPAVRLLKSVGIPKGTEMVKRFGITNPMAPFLPSALGATEVPLV
                     EMVSAYSVFPNKGTRARPHYIRRVTDRNGITIYDYANDEKQQAFKVLSPYVAGQMVTL
                     MRGVVESGTAAAIKGVTQGDLNKREIAGKTGTVNDYTDAWFIGYTPSVACGCWIGHQG
                     EKRSLGEGETGGSAALPMWTKFMGFYMKGKPIEKFGEVPPPDEKTKSMIAARTREAQK
                     ELEKMAEESAIDTGADLAKEAIMGDGDSEAPPKPSKPKDPAKSGTDDKKKPAAGDDKK
                     KPSTTAGADKKKPSTSTGWVKPGDKPPAAKPKKPNQ"
     gene            2903..>3425
                     /locus_tag="PKZ53_07880"
     CDS             2903..>3425
                     /locus_tag="PKZ53_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ40393.1"
                     /translation="MKNNHSGSLVQLQEVLRKDLPLSVVLGGGGVRGLAHLGVLEVLS
                     EQGFQLAEIVGTSVGALILTYYAAVGLDVPTMRKLGLGMSSRHLLGWAFLRRFSPPIQ
                     HRFRRLAGIIPEYLDRLSNLPGDRLFHGVERIGLVAYDLECREEIVFHNLQQEFLLND
                     ALRGAVAIPGIFPP"
BASE COUNT          914 a          859 c          880 g          772 t
ORIGIN      
        1 gaaccgatac cttccaagaa ccaagaacca agaaccaaga accaagaact aagtctcttc
       61 attcttcatt cttcattctt cattcttcat tcttcattct caactcttca ttctcaattc
      121 ttcattcagc ctgcagtgag gtggttgcat gcctgtaaac agtattttta ctcgatctga
      181 attggatgat tctcgaaaga aaaaaccggc gatggtccag cggttttttc ggaaatctac
      241 attctttgcg gtgttgttac tctcggcgct ggcaggcagc ctggccgggc tggccgtggc
      301 gtatcaaagc agcctggctg gtcaggattg gggggtggct gagctggcca gctatcagcc
      361 gtggggaatt acccaggtct atgcctcaga tggaaaatcg gttatcggtg aatttgccct
      421 tgagcggcgc attccgctca gctatgacga aattccgctg catatgcgcc aggccattct
      481 cgccatcgaa gatgcccggt ttgaaaaaca tcttggggtt gacccaattg gaatcagccg
      541 ggctctggtc aaaaacacgc tcgctgggcg gacggtggaa ggtggctcaa cgctgacaca
      601 gcaactttcc aaaatgctgt ttttgactga cgaacgcacc tatacccgca aaatcaaaga
      661 agccattttg tcattgcaga tcgagcggga atttagcaaa caacaaatct ttgaactgta
      721 tgccaatcag atcaatctgg gaggcggggc ctacggcgtt gaagccgggg ctcaatatta
      781 tttcggaaaa tctgccaaag atctgaccat cgaagagtgc gccacgcttg ccgccattcc
      841 caaagcgcca tcaaactatt cgcccgcgct caatcctgaa aaatcattaa atcgccgcaa
      901 cctggtgatc aacaacatga ttgaagaagg atttatttct gctgctgagg gggaagccgc
      961 caaaaacaag ccaattaagc tcaacctgac cccgcgtgaa accaacacca gcggccccta
     1021 cgcgtttttt gtcgaagaag tccggcgcga acttgaaaac aaattcggat cccgcgaaac
     1081 gcactccctc ggctataaag tcattacaac cgttgatgct gaagctcaag tcagagctgt
     1141 ccaggcggtt cgtgccgggt tgcacgctta tgcccagcgc cacggtgcca aatggcatga
     1201 tgacctcgac aacatctttg atcagggtgt gaaagacatc aacaaataca aacacccgga
     1261 atggagcctc accctcgctg aaggcatgta tgtgtatgga ctcattaccg aaatcaatag
     1321 tcgtgaggcg aaagtgtcgt ttggcgacta tcacgccgtg gtcactgaaa aagagattgc
     1381 cattgctggt ggctctccgt ctaaggtttt taaagtcggt gatttagctg tctttcaaat
     1441 caaaaaaatg aaccagcctg agaaagacaa agaagccgaa aagaaagaag caaaaccaaa
     1501 gactgaatcc actggtgaca aagacgcgaa gaaaaaaccc aaagccgaac cacagggtga
     1561 cccgtttgaa ttgtcggtcg aattacaggt cgttccaaaa gttacgggcg cattgttgtg
     1621 tattgaagcc aaaactggcg aagtcactgc catggttggc ggatatgatt tctcaatctc
     1681 gcggtttaac aacgccaccc agggaaatcg ccagaccggt tctgccttta aaccttttat
     1741 ttatgccacc gcagttgaaa atggctggaa accagagagt ctggtccttg ataccccatt
     1801 tacccaggga aactggtcac cgcgtaacta tgacggttca ttctccgggg cgattccgct
     1861 gagcaccgca ctggccaaat cacggaacat tcccgccgtt cgccttttaa aatcagtcgg
     1921 aattccgaaa ggaacggaga tggtcaaacg gtttgggatc accaacccaa tggccccgtt
     1981 tctcccctcg gcgcttgggg cgactgaagt cccgctggtc gaaatggtgt cagcctactc
     2041 ggttttccca aacaaaggga ctcgggcacg tccacactat attcgtcggg tgactgaccg
     2101 gaatggaatt accatttatg actatgccaa tgatgaaaaa cagcaggcat tcaaggtgtt
     2161 gtctccctac gtcgccggtc aaatggtgac cttaatgcgt ggcgtagttg agtctgggac
     2221 ggcggcggcg atcaaaggcg tgacccaggg cgatctgaac aaacgtgaaa tcgctggtaa
     2281 aaccggtacg gtcaacgatt acaccgatgc ctggtttatt gggtacacgc cgtcagttgc
     2341 ctgtggttgc tggattggac accagggcga aaaacgctcg cttggcgaag gtgaaaccgg
     2401 cggcagtgcg gccctgccca tgtggacgaa atttatgggc ttttatatga agggcaagcc
     2461 gattgaaaaa tttggcgaag tcccgccgcc tgacgaaaag accaaatcaa tgattgccgc
     2521 tcgaacccgc gaagcccaga aagaacttga aaaaatggcc gaagaatccg ccattgatac
     2581 gggtgctgat ttagccaagg aagcaatcat gggcgatggt gatagtgaag ccccaccaaa
     2641 accatccaaa cccaaggatc cggccaaatc tgggactgat gacaaaaaga aacccgccgc
     2701 tggtgacgac aaaaagaaac catccacgac tgcaggcgcc gataagaaaa aaccatccac
     2761 ttcaaccggc tgggtcaaac cgggtgacaa gccacccgcc gccaaaccca agaaaccaaa
     2821 tcagtaaagc caatgaagaa tgaagaattg agaatgaaga attgagaatg aagaattgag
     2881 aatgaagaat tgagaatgaa gaatgaagaa caaccattct ggttcgctgg ttcaacttca
     2941 ggaagtactt cgtaaggatt taccactcag cgttgtcctg ggcggtggtg gcgtgcgtgg
     3001 actggcccat cttggtgttc tcgaagtcct ttctgaacaa gggtttcaac tggccgagat
     3061 tgtcggtacc agtgtcggcg cgctgatttt gacctactat gccgccgttg ggcttgatgt
     3121 gccgacgatg cggaaattag gtctggggat gagttcacgg cacttgctcg gatgggcatt
     3181 tctgcggcgg ttttcgcctc ccatccagca ccggtttcga cgtctggcag gtatcatccc
     3241 ggaatatctg gaccgacttt ccaatctgcc cggtgaccgg ttgtttcacg gcgttgagcg
     3301 gattgggctg gtggcgtatg accttgaatg ccgcgaagaa atcgtcttcc acaatctgca
     3361 gcaggaattt ctgctcaatg atgcactgcg tggggcggtg gcgattcctg ggatttttcc
     3421 acctc
//
LOCUS       DAOQDO010000410         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20796_length_3423_cov_3.678741, whole genome shotgun sequence.
ACCESSION   DAOQDO010000410 DAOQDO010000000
VERSION     DAOQDO010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20796_length_3423_cov_3.678741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..3330
                     /locus_tag="PKZ53_07885"
     CDS             100..3330
                     /locus_tag="PKZ53_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ40394.1"
                     /translation="MRRWLWLALVFGLWSLSVGGQPSEKRLYAQVTANQTQIVFSLAG
                     DLWTVDKAGGSARQLTNTPGEEGMPAFSPDGQHLAFSRQSGGNWDIYLMPASGGEARR
                     LTFHPRNDVVVGWHPDGKHILYRSSASGVPKLYSLAVDGVLPTELPFDRALTGSYSPD
                     GNRLALNPNLSLSDWRYYRGGYQGQIWLTEVKTGVTERVPQLDFNNDMPVWLGDMIYF
                     LSDRTGVHNLFAFDVKTRRTTQLTTFEQFGIQTLGQGPQSLVFVRDGQLYLFELDKPG
                     PVPVTVSLTAPAPERLPRTVDASPLIDSVAFGMSGDSLVVGARGEIFLVEPATGKKTN
                     LTQSSGVAERWPVLSPDGKWVAYFSDETGEYQLCVKATSGTGSIKRLAIEAKPSFYRD
                     LLWSPDGKKLVFCDRRLGVWLAEPDQEKVSRIDTTTYSYQIDWHLNWSPDGRWLAYSK
                     SLASRIRTIFVYDTDHRQLHQMTSGKIWAEFPCFDAGGKYLYFVSSPNAALSGYGWGV
                     LNGVMARPGITRRLHLVSLRESDPLPFLPDGKPNPAAKTDERLTSTTIDFANLATRMV
                     NVPIPEQDFAQLLPGKPGVVFALTNEWPSPPVFTARPRRTLSQWNLTSASTFEKIAEN
                     TPLATVSGDGTTLFYQADRDWILVPTVNAPSAEKKKLDLSKLELNLDPSAEWKQLYRE
                     SWHIMRDWFYDPNFHGQDLAFLEHHYAAYLPGITRRADLNTLLNRMLGHVSVSHLVVD
                     GGDVPPPAGQPSRGGLLGADFEIAEGKYRFRHILRTSTLEDPLGLVPAPLDRPDARVA
                     DGSFVLAIDGKPVEISKPIDLYLEGKANRPIQLTVASDASGRNSQTLMIYPIANEATL
                     RAANQVKAFQQRVEEASQGQLGYVYVANYGAATMDAIRGLLGNSQRKGVIIDQRYNGG
                     GITPDFLIEWLQRKPLYFYTFREGDDIATPVNPGPQVKVLMTNELNGSAAETFAFMFK
                     LAKLGPIVGKRTFGGGIGPYFPTPDLLDGGFVQLPNRAAYQSDGTSWGIENIGVSPTI
                     EVDILPADVLAGRDPQLEKAIEAALAELKKIKTPAVRKPKYPVHPK"
BASE COUNT          791 a          866 c          927 g          839 t
ORIGIN      
        1 ggcatctgac ccaagaattc ctgggagcgc tggcatcctg ccggcatctg aacatagcag
       61 gtgacgttgg tggaaacttc aaaatcgagg aatggaacta tgagacgatg gttgtggctg
      121 gcgttggtgt ttggtctgtg gtcactgtcg gttggtgggc aaccgtctga aaagcgactg
      181 tatgcacagg ttacggcaaa ccaaacccag attgtattta gcctggctgg tgatctatgg
      241 acggttgata aagctggcgg cagtgcccgg caactcacca atactcccgg cgaagaaggc
      301 atgcccgcct tttctcctga tgggcaacac ctggcttttt cacgccagtc aggcggtaac
      361 tgggacattt acctgatgcc ggccagcggt ggcgaagccc ggcggctgac gtttcatcca
      421 cgcaatgacg ttgttgtggg atggcatccg gatggaaaac atattctcta ccgatcaagt
      481 gccagtggtg tgccaaagct gtactctctg gccgtggatg gtgtcttgcc gactgaattg
      541 ccctttgacc gcgccctcac tggatcatat tcgccggatg gaaaccggct tgctttgaat
      601 ccaaacctct ctttgagcga ctggcgatac tatcggggtg gctatcaggg ccaaatctgg
      661 ctcactgaag tgaaaacagg cgtcacggaa cgggtgccac aactggattt caataatgac
      721 atgccggtgt ggcttgggga catgatctat tttttgtctg atcgaaccgg tgttcacaac
      781 ctttttgcct ttgatgtgaa aacccggcgc acaacacaac tgactacgtt tgagcaattt
      841 ggaattcaaa ctctgggcca ggggccacaa tcgctggttt ttgtccgcga tggccagttg
      901 tatctttttg aattggacaa accgggtccg gtaccggtaa ctgtctcact cactgctcca
      961 gcacctgagc ggttgccgcg aacggttgat gcctcgccat tgattgattc agttgcattt
     1021 ggaatgtcgg gtgattccct ggtggttggc gcacgaggcg agatctttct ggttgaacca
     1081 gccactggga aaaagacaaa cctgacgcaa tcttcaggcg ttgccgaacg ctggccggtt
     1141 ttatcgcccg atggaaaatg ggtcgcctat ttctcagacg aaacgggtga atatcagttg
     1201 tgtgtgaaag cgacatccgg aacaggatcc atcaaacggc tggctatcga agccaaacca
     1261 tcgttttacc gcgacttgct ctggtctccg gatggcaaaa aactggtctt ttgtgaccgt
     1321 cggcttggcg tctggctggc tgaacctgat caggaaaagg tatctcgaat tgataccaca
     1381 acctattcat atcaaattga ctggcatttg aactggtcgc cggatgggcg ctggctggcc
     1441 tattccaaaa gtcttgccag ccggattcgg accatctttg tgtatgatac cgaccatcgg
     1501 caactccacc agatgaccag cggaaaaatc tgggctgaat ttccatgttt tgatgcgggt
     1561 ggaaagtacc tgtattttgt ttcaagtcca aatgccgcgc tcagtggtta tggatggggt
     1621 gtcttaaatg gggtaatggc tcgtccgggc attacgcggc gattgcacct ggtttccctt
     1681 cgggaatctg acccgctccc atttttaccg gatggcaaac caaacccggc ggcaaagact
     1741 gacgaaagac tgaccagtac cacgattgat tttgccaatc tggccacccg gatggtcaat
     1801 gttcccatac cagagcagga ttttgcgcaa ctcctgcctg gcaaaccggg cgtggtgttt
     1861 gcactcacca atgaatggcc gtcgccgcca gtgtttacgg cccggccacg gagaaccctt
     1921 tcccagtgga atctgacatc ggccagtaca tttgaaaaga ttgccgagaa tacgcccctg
     1981 gccactgttt cgggtgacgg tacgaccctg ttctatcagg ctgatcgaga ttggatttta
     2041 gttccgaccg taaatgcgcc ttcggctgaa aagaaaaagc ttgatctttc aaaactggaa
     2101 ctgaacctgg atccgtccgc cgaatggaag caactgtatc gtgaaagctg gcacatcatg
     2161 cgcgactggt tttatgaccc aaattttcat ggtcaggatc tggcgtttct cgaacaccac
     2221 tatgcggcgt atttaccagg catcacccgt cgggctgatc tcaacaccct gttgaaccga
     2281 atgctgggcc atgtttccgt cagtcacctg gtggtagatg gcggagatgt tccgcctccg
     2341 gctggccagc catctcgtgg aggtctcctc ggggcagatt ttgaaattgc cgaaggaaag
     2401 taccggtttc gccacatcct cagaaccagc acccttgagg atccgctcgg gttggttccg
     2461 gcgccgcttg accggcctga tgcccgcgtg gctgatggtt cgtttgtcct ggcgattgat
     2521 ggaaagccgg tggagatttc caaaccgatt gatctgtatc tggaaggcaa agccaaccgt
     2581 ccgattcaac ttacggttgc ttccgatgcc agtgggcgca attcgcagac gctgatgata
     2641 tatccaattg ccaatgaggc cacgcttcgg gcggccaatc aggtcaaggc atttcaacaa
     2701 cgggttgagg aggcttctca agggcagctt ggctatgtgt atgtcgccaa ttatggggcg
     2761 gcgaccatgg atgcgattcg gggattgctt ggaaacagcc agcgaaaagg tgtcatcatt
     2821 gaccagcggt ataatggagg cgggatcacg cctgattttt tgattgaatg gttgcagcgt
     2881 aaaccgttgt atttttatac cttccgtgaa ggagacgaca ttgccacccc ggtcaatccc
     2941 ggcccgcaag tgaaagtctt gatgaccaat gaactcaacg gctcagcggc tgaaacgttt
     3001 gcctttatgt tcaagcttgc caaactggga cccattgtcg gaaagcgaac ctttggcggt
     3061 ggcatcggtc cctatttccc aacaccagac ctgcttgacg gaggttttgt ccagttgccc
     3121 aatcgagcgg cctaccagtc agatggcacc tcctggggaa ttgaaaacat cggtgtcagc
     3181 ccaaccattg aggttgacat cctgccggct gatgttttgg ctggccgaga ccctcaactt
     3241 gaaaaggcga ttgaagcagc actggccgag ctgaaaaaaa taaaaactcc agcggttcga
     3301 aagccaaaat atccggttca tccgaaataa taccaattag ggttcagggt tcagggaaat
     3361 acaattctct caatagttta gccttcccgt aacacaaagt gttcagtgca aacacaattc
     3421 aat
//
LOCUS       DAOQDO010000411         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20811_length_3422_cov_2.805168, whole genome shotgun sequence.
ACCESSION   DAOQDO010000411 DAOQDO010000000
VERSION     DAOQDO010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_3422_cov_2.805168"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKZ53_07890"
     CDS             <1..575
                     /locus_tag="PKZ53_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40395.1"
                     /translation="TFLLEAARRKLQLGSLLVPTTVLVLQPDDAGGGWLWTISPWMKT
                     LRTLMNQALATANENALGLALEIFARAAVRALVLASRQNLVLDVHPSNFAVNDDQVTF
                     VYIDDDIGSGSLIPAVSYCFLRRFDEYLEYSQALMAYLEAVEVAMEMRLERADVKVLN
                     LIEDFEHCQVRSGLAEQARSRIVKILRRRQ"
     gene            593..1339
                     /locus_tag="PKZ53_07895"
     CDS             593..1339
                     /locus_tag="PKZ53_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNJ40396.1"
                     /translation="MRLAVLSDIHSNIAALEAVAEAIDRLLPDEIICLGDIVGYNAQP
                     AECVRFIQNLTPLVIAGNHDRDMISGMALQGTNKAAHLVQDWTRQHLNDEELDYLASL
                     PSHCVQPEYVAVHGCYLNTTYVNGYVTSTMLEENLHAICERATWPRLAFCGHTHVPMC
                     GWLEGTGCIEPRVPQSVRWPASAQVVLINPGSVGQPRDLDPRASFALVDTQAHTLEIH
                     RVAYDIDRTIEAIKQAKLPVELGERLRIGR"
     gene            complement(1457..3370)
                     /locus_tag="PKZ53_07900"
     CDS             complement(1457..3370)
                     /locus_tag="PKZ53_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40397.1"
                     /translation="MKISRQPFEAIFQISPFGLAILDVQGTVLRANDHLAYLLQVQLT
                     EQLEGHQLIQWLDSSQAAFLNQFNQLTTETISEFSEEVVFESSFQTQVVAQVHGVLIE
                     WENHPAVLLQVQPGKYQLSQTENEFQELRNQFDTIIELSSDAFIGIDQSQTITSFSPG
                     AEKIFGYHVGEVLGRSLNILIPSRFHPTHQTHIQHFAIGPNTIRPMNERGPIVGLRKN
                     GQEFPAEAAIQKLQTSSGVHLKVRLRDLSDRVFLENQLRQAQKMEAIGQLASGIAHDF
                     NNLLTVISGCSEILLLRMRATDGLRPRVEEIRRSAERATSLIRQLNTFTQRRILETKS
                     INLNSVINELNLMLQRLIGEDIELNRHLEPALDLIRADIGQIEQVIVNLVVNARDAMP
                     RGGKISIRTANCSIQPGNETAENPVPPGQYVQLSIQDSGCGMPQEVVDRIFDPFYTTK
                     DPGKGTGLGLSTVYGIVKQYNGHITVDTQLNQGTTFHIFLHRTEHVTPQEVVKAIITE
                     PSRGSETILLVEDEQSVRVMISETLQMYGYKVLEAANGQIAIEIQASYPHPIDLLISD
                     LVMPVMDGSELARRLLKKRPDLKVMFMSGYTERYSHKLIRQTQNLSVDYLEKPFSLDQ
                     LLLRIRRVLDHSP"
BASE COUNT          830 a          757 c          886 g          949 t
ORIGIN      
        1 gaaccttttt gctggaggca gcccgtcgaa aattgcaact ggggtcgctt ttagtcccaa
       61 ccacagtgtt ggttttgcag cctgatgatg cgggcggagg ctggttatgg actatttcgc
      121 cctggatgaa aacgttacgg acattgatga accaggccct ggctaccgcc aatgagaatg
      181 cgttaggcct ggcacttgaa atatttgccc gggctgctgt tcgggcgtta gtactggcat
      241 cccgtcaaaa tctggtgctg gatgtacatc caagtaattt tgccgtcaat gacgatcagg
      301 ttacgtttgt gtatattgat gacgacattg gcagcggttc gttaattcct gcggtaagct
      361 attgttttct tcgacgtttt gatgagtatc tggagtatag tcaggccctg atggcgtatc
      421 ttgaggctgt tgaagtggca atggagatgc gtttagagcg ggctgatgtt aaagtcttga
      481 atttgatcga agactttgaa cattgtcagg ttcgatctgg attggcggag caggctcgat
      541 ctcgaatcgt aaaaatcttg cggcgacggc agtgatcatt caagctgaca ctatgcgact
      601 agctgttctc tccgatattc actctaatat tgctgcgctt gaggctgtgg ccgaagccat
      661 tgatcgcctt ttacccgacg agattatttg cctgggtgat attgttggat acaatgccca
      721 accggctgaa tgtgttcggt tcatccaaaa cctgaccccg cttgtcattg ccggaaacca
      781 tgatcgggat atgatttctg gaatggcact tcagggaaca aacaaagccg cgcatctggt
      841 tcaggattgg acacgtcagc atttgaacga tgaggaactc gattacctgg caagtctgcc
      901 cagtcactgt gttcagcctg aatatgttgc tgtccatggg tgttatctca atacaaccta
      961 tgtgaatggg tatgtgacca gcacgatgct tgaggaaaat ttacatgcca tttgcgagcg
     1021 ggcaacctgg cctcgactgg cgttttgcgg tcatactcac gttccaatgt gcggttggtt
     1081 ggaaggcact ggatgtattg aacctcgggt gccacaatcg gtccgctggc ctgccagtgc
     1141 tcaggtggtg ttaatcaatc ctggttcggt tgggcaacct cgcgatcttg atcctcgggc
     1201 gtcctttgcc ctggttgata cccaggccca cactctcgaa atccatcgtg tggcctatga
     1261 catagaccga accattgagg cgattaagca ggcaaagctt cctgttgagt taggggaaag
     1321 attgcgaatt ggccgctgat gaggttccat cgctgaagaa ggtgaccgga aggttttcag
     1381 cctcagggtt aaacaacctg gtttcacttt cctgggcccc aagcccccag acccagggcg
     1441 atagttcgct ggttgctcac ggagaatgat caagtacgcg cctgattcga aggagcagtt
     1501 gatctaacga gaagggtttt tccagataat caactgatag attctgagtc tgtcggatga
     1561 gtttgtgtga gtatcgctcg gtatacccgg acataaacat gactttcaga tctggtcgtt
     1621 tcttgaggag ccgcctggca agctcactgc catccatcac aggcataacc agatcggaaa
     1681 tcaacaagtc gattggatga gggtagctgg cttggatttc aatggctatc tgtccatttg
     1741 cggcctcaag tactttatat ccatacattt gtaacgtttc tgaaatcatg acacgtactg
     1801 attgttcgtc ttcaaccagc agaatagttt cagatccgcg actgggttca gtgataatgg
     1861 ctttaacaac ttcttgtggg gtcacatgtt cggtacgatg caggaagatg tgaaatgtgg
     1921 tgccctgatt gagttgcgta tcaacagtga tatgaccgtt atattgtttc acaattccat
     1981 acactgtaga caggccaagc ccggtgcctt tgcctggatc ttttgtcgta taaaacggat
     2041 caaaaatacg atcaaccact tcctggggca tgccacatcc gctgtcctga atacttaact
     2101 gcacatactg gccaggggga acggggttct ctgccgtttc atttcctggc tggatcgagc
     2161 aattcgccgt ccgaatcgag atttttcccc cacgaggcat ggcatcgcgg gcattgacga
     2221 ccaggttgac gatcacctgt tcaatctgcc caatatcggc ccgaatcaga tcaagtgctg
     2281 gttctaaatg acgattcagc tcaatgtctt caccaatgag gcgttgaagc attaaattaa
     2341 gttcattaat aactgaattg agattgatgg atttggtttc gagaattcgt cgctgggtga
     2401 aggtattgag ctgtcgaatg agagaggtgg cgcgctcggc agaccggcgg atttcttcaa
     2461 ctcgtgggcg aagcccatca gtggctcgca ttcgcaagag caaaatctcg ctacacccag
     2521 aaatcacggt cagcaggtta ttaaaatcgt gggcaatccc gctcgcgagt tgcccaatgg
     2581 cttccatttt ttgggcctga cggagctgat tttccagaaa aacacgatct gagagatcac
     2641 gaagccggac tttgagatga acaccagaac ttgtttggag cttttgaatt gccgcttcgg
     2701 ctggaaattc ctgaccattt ttccgcaatc caacaattgg cccccgttca ttcattggcc
     2761 ggattgtgtt tggaccaatc gcaaaatgtt gaatgtgtgt ttgatgagtt ggatgaaagc
     2821 gcgatggaat taaaatgttg agtgacctgc caaggacttc gcccacgtga tagccaaaga
     2881 ttttttctgc tcctgggcta aaggaagtga ttgtttggga ctggtcaatt ccaataaaag
     2941 catcacttga aagttcgatt atggtatcaa actggtttcg gagttcctga aattcatttt
     3001 ccgtttgtga gagttgatat tttcctggtt gaacctgaag taaaaccgct ggatggtttt
     3061 cccactcgat caaaacacca tgtacctggg ccacgacctg ggtttgaaag ctgctttcaa
     3121 aaacaacttc ctctgaaaat tcagaaattg tttcagtggt aagctggttg aattgattga
     3181 gaaaagccgc ctgcgatgag tcaagccatt gaatgagttg atgcccttca agttgttccg
     3241 ttagctgcac ttgtaggaga taagccagat gatcgttcgc tcgaagaaca gtgccctgaa
     3301 cgtcaaggat ggctaaccca aaaggtgaga tttggaagat agcctcaaat ggctgacggc
     3361 ttattttcat tgatgtctaa ctgggcaatt caagatgggg agaagatctt actgtacttt
     3421 ta
//
LOCUS       DAOQDO010000412         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20876_length_3417_cov_2.397680, whole genome shotgun sequence.
ACCESSION   DAOQDO010000412 DAOQDO010000000
VERSION     DAOQDO010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20876_length_3417_cov_2.397680"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1111)
                     /locus_tag="PKZ53_07905"
     CDS             complement(131..1111)
                     /locus_tag="PKZ53_07905"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNJ40398.1"
                     /translation="MYSAGIVGTGHMLPEKVLTNADLETLVETSDDWIMTRTGIRERH
                     IAGVGETTSMIASAAAQQAMDAAGLKANDIDLILCATVCPDMALPSTACFIQSRLGAK
                     KAAAYDLVAACSGFLYGVTTANAFIATGQAKNVLVIGAEILSRYVDYTDRSTCVIFGD
                     GAGAAIMTRVETGRGILASRIQSDGDFADYLFTPGGGTLHPASEQSLENRLHFIKMRG
                     NELFKVAVRSMTETSRQVLDEVKMTAADIDLLIPHQANQRITDAVADRLQIPPTKVYS
                     NISRIGNTSSASIPIALDECVRSGRVQRGNVLMFSSFGAGATWGSMVVRW"
     gene            complement(1263..2300)
                     /gene="plsX"
                     /locus_tag="PKZ53_07910"
     CDS             complement(1263..2300)
                     /gene="plsX"
                     /locus_tag="PKZ53_07910"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNJ40399.1"
                     /translation="MLRVAVDALGGDHAPASELEGSLQAARQLGVHIVLVGPSGLIEN
                     ALRDLGWTNEPIEIVNATEVITMGDDVARAVRRKKDSTIRVCMQLLRDGRVDAVVSAG
                     NTGAVMMTSALLLGTLDGVERPALAMMLPTVNGQGTLLLDVGANADCKPAFLEQFAVM
                     GGLYAEKILHHPRPRVGLLSIGEEESKGNEVTREAHKLLKSSSSIHFVGNIEGKDLWT
                     GSVNVAVCDGFTGNVVLKTSEGLIDAIQKMLKEEMMRRIDTQAGAYLVRPAFQAFKKR
                     LDYEEYGGAPLLGARGTVIICHGRSTAKAIRNAIRVAKESLQLEVNRLIESELAETYQ
                     VSSKRRNSSKP"
     gene            complement(2406..2600)
                     /gene="rpmF"
                     /locus_tag="PKZ53_07915"
     CDS             complement(2406..2600)
                     /gene="rpmF"
                     /locus_tag="PKZ53_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNJ40400.1"
                     /translation="MPNPKRRHSSSRRGKRRAHDALSPMMLITCSNCGVQIQAHRACD
                     KCGYYKGRQVIAVRSVTEAE"
     gene            3064..>3417
                     /locus_tag="PKZ53_07920"
     CDS             3064..>3417
                     /locus_tag="PKZ53_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNJ40401.1"
                     /translation="MIRNLKFDDRGLLPTTVQDTRSHQILVQDFMNAESLALTVQSGT
                     VWFYSASRNEVWCSRSSFGQVHRVVDIAPDPEGTGLRIQVEVGDPAVDESKSPDARRA
                     RSLSAVPPTRSGVSDP"
BASE COUNT          817 a          885 c          911 g          804 t
ORIGIN      
        1 taaagaaacc acggaataca cggaacacac agaaaataaa tccaaattct tcaatggttt
       61 ctgagacttc ccaacgcaaa cactcaggaa atatttatct aaaaggctct atttcgctct
      121 ttcgcttgat ttaccaccga accaccatgc tgccccaggt ggcacccgca ccaaaggaag
      181 aaaacatcag gacgttgcca cgttggacgc gacccgaccg gacacattcg tccagagcaa
      241 ttggaatcga agctgaagag gtatttccga tccggctgat gttggaatag acctttgtgg
      301 gtggtatctg taaccggtct gcgaccgcat cggtaatgcg ctgattggcc tggtgtggaa
      361 tcaacaggtc aatgtcagcc gccgtcattt tgacttcgtc cagaacctgg cgtgaagttt
      421 cagtcatact gcggacggcc accttaaaca attcattgcc acgcatcttg ataaagtgaa
      481 gccggttttc aagcgattgc tcgctggctg gatgcagggt gcctccgcct ggcgtgaaca
      541 ggtaatcggc aaaatcaccg tcgctttgga ttcggctggc cagaatccca cgcccagttt
      601 ccacccgggt cataatcgcc gccccggccc catcaccaaa gatcacacag gtggatcggt
      661 cagtataatc aacatagcgg gacaggattt cagcgccgat caccagcacg tttttggcct
      721 gaccggtggc aataaaggca ttggccgtgg tgacaccata taaaaaccct gagcaggcgg
      781 ccaccagatc ataggcagcc gcttttttgg cgcccagtcg agattggata aaacaggcgg
      841 ttgacggcaa ggccatatcc gggcaaacgg tggcgcacaa gatcagatca atgtcgttgg
      901 cttttaaccc ggcggcatcc attgcttgct gggcggcagc cgaagcaatc atggaggtgg
      961 tttcgccaac cccggcaatg tggcgttccc gaatgccggt gcgggtcata atccagtcat
     1021 cagaggtctc gaccagtgtc tcaagatcgg cattggtcaa aactttctct ggtaacatgt
     1081 gcccggtacc gacaattcct gctgaataca taacactgaa tctccttgga aactgaactg
     1141 cgggctgaag aaattgggct gaagatcatc gggctgaaga agttgggctg aagatcatcg
     1201 ggctcagggg attcaacccg ttttcttcag ccctaagccc gacttcttca gcccctggcc
     1261 ttctagggtt tagaggaatt ccggcgcttg ctggaaactt gataggtctc tgcaagttcg
     1321 gattcgatca gccgattgac ttcgagttgg agtgattctt tggcaacccg gatggcgttg
     1381 cggatggctt tggcggttga tcgcccgtga cagataatga ccgttcctcg tgctcccaac
     1441 aagggagccc caccatattc ctcataatcc aggcgttttt taaatgcctg aaatgctgga
     1501 cgcaccagat aggcacccgc ctgggtatca atccggcgca tcatttcctc tttgagcatc
     1561 ttttgaatgg catcaatgag tccttcgctg gttttgagaa ccacattacc ggtaaaccca
     1621 tcacacacgg caacattcac ggagccggtc cagaggtctt ttccttcaat gttgccaaca
     1681 aagtgaattg aggaggagct tttgaggagt ttgtgggctt cgcgagtgac ttcattgcct
     1741 ttgctttctt cttcaccgat agacaatagt ccaacgcgcg ggcgaggatg atgcagaatt
     1801 ttctctgcat agagcccacc catcaccgca aattgttcga ggaaagcagg tttgcagtca
     1861 gcatttgccc cgacgtcgag cagcagtgtt ccctggccat taaccgtagg cagcatcatg
     1921 gccagggctg gacgttcgac tccgtcaagc gtaccgagca gaagcgcact ggtcatcatg
     1981 acggcaccgg tgtttccggc actgaccacc gcgtccactc ggccatcgcg gagcaattgc
     2041 atacacacac gaatggttga atcttttttg cgccgaaccg cccgggccac atcgtcgccc
     2101 atggtgatga cttcggtcgc attgacaatt tcgattggtt cgttggtcca ccccaggtca
     2161 cgaagtgcat tttcaatcaa cccggatggc ccaaccaaca caatatgaac gcccaattga
     2221 cgggctgctt gcagagaacc ctctagttcg ctggcgggtg cgtggtctcc gcccaatgca
     2281 tcaacggcaa ctcgcagcat acgtgttttg ataattccta aaactgaaag gattcgacgt
     2341 ggcgcccgtc ggagaggggc aggttcgaaa aaaaccttcc tgcggggcca gtcgggaagg
     2401 cagggttatt cagcttcagt aacgctgcgg acggcgatca cctggcggcc tttatagtaa
     2461 ccgcacttgt cacaggcgcg gtgggcttga atttgcacac cacaatttga acaggtaatc
     2521 agcatcattg gggacagcgc atcgtgagcg cggcgttttc cacggcggga tgaagaatgg
     2581 cgtcgttttg gatttggcat gacctttact ctcctggcac aaagtcttga aaagaataga
     2641 tttttttggc acttgagcta tccattccga agcaggacct gggatgtgat ttccgtgtct
     2701 gatgggcaaa aagcacgctc aagtcccaaa aattgacccg aagtcagacg acattttgtg
     2761 gatcaggagc ggtgcagatt ggggcaacca gacaggtgag tggaaggcat catcatgggt
     2821 gaaaccactc gggcggaagc caaggagcgc aaaagtgcgt tattataagg gcatacgcgt
     2881 ttttgtcaat cgcagaagca gcagagcgtg cggcatgaac cgggggtgct tgtccgaagg
     2941 ggcgcgttgt ggtagttcta agatacacgc actgagttct cgttcttcga ttccaggtac
     3001 cagattctca attcccaaat accagattca atgtgatttg agtccgacga caggttattg
     3061 attatgattc gaaacttgaa gtttgatgac cgggggttac ttccaacaac tgtccaggat
     3121 actcgttcgc accaaatttt agttcaggat tttatgaatg ccgaaagtct ggcactgacc
     3181 gttcaatcgg gaacggtgtg gttttatagt gccagtcgca acgaagtttg gtgttcgcgt
     3241 tcgtcatttg gtcaggttca tcgcgtggta gacattgccc cagacccgga aggcaccgga
     3301 ctgcggatcc aggttgaagt cggtgacccg gcagtggatg aatcaaaatc acctgatgcc
     3361 cgccgtgccc gttcattgtc agccgtacca ccgacccgat ccggggtgtc agacccg
//
LOCUS       DAOQDO010000413         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20884_length_3416_cov_2.581077, whole genome shotgun sequence.
ACCESSION   DAOQDO010000413 DAOQDO010000000
VERSION     DAOQDO010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20884_length_3416_cov_2.581077"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="PKZ53_07925"
     CDS             <1..2039
                     /locus_tag="PKZ53_07925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40402.1"
                     /translation="RIWSVLVDRQGQVWVGTESGLDRLDRATGTFTRFQEKAAETGRP
                     VEDNINVLYEDRAGVVWVGTYGNGLLRLDPTTKEVTRYLSAAWNATSLGSNTVFTIYE
                     STAGALWVGTNGGGLSCLDRTTGTFTSYTIRDGLPSNSVMSMLEDEAGNLWLGTTLGL
                     CRFHPPSRSCRNYDSRDGLQSNEFTQNAALKTSRGEMLFGGINGFNWFHPEKIEDRQF
                     IPALSLTSFLIYGRELEGFNGKDLPPLDYTQNFITFKFAFLNDTVPEKNRYQYKLEGF
                     DRDWVLSGSQRYASYTDLKPGTYVFRVKGANSDGVWDQQGIALRFEVIPPPWKTWWAY
                     SLYVVGIVGGSWLGVQLRLRSLKRRAIILEQSVIERTQEVVQQKNELSHQNEQIIRQR
                     DEIERKSAELERRSREIQEKNSQIIDSILYAERIQQAILAYRKRIGQSLSEYFILFRP
                     KDIVSGDFYWFHYVDGISIIAVVDCTGHGVPGAFMSMIGNSLLSQIVIENQVHDPAQI
                     LELMHVGIRKALKQDADDAESQDGMDVAVCRIDPLQHTIIFAGAKRPLYYINGGGELI
                     EVKGDRKSVGGKQKETQRKFTNQTIPIQKEVTVYLASDGFSDQAGETSRKFGTRRFKE
                     LLQSTSGYSLEVQYHALIEELEAHQGTEPQRDDITVVGVRVQAERNYSPEGYLM"
     gene            complement(2265..2645)
                     /locus_tag="PKZ53_07930"
     CDS             complement(2265..2645)
                     /locus_tag="PKZ53_07930"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008319565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNJ40403.1"
                     /translation="MPSKFRTPGYHPLRKIKVAFAGLYVAVLTDFSVAYKVVLSLPVL
                     IVAFFFRQWVDLSIILLATGLMLIAELFNSSIEVLCDFVEQREDEKIRIIKDIAAAAA
                     GISIVVWATILVLESLHIWGIAKP"
     gene            complement(2816..>3416)
                     /locus_tag="PKZ53_07935"
     CDS             complement(2816..>3416)
                     /locus_tag="PKZ53_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40404.1"
                     /translation="SSAGPQTVAVIQSPQARPGQLFAEFRKATLVMLQSPSGLNRPIL
                     EQSLIKLAQSQFSIAAIGTTSLEWTSSEINGLPFRQLNLPMLGLTWCYALKDRALVLA
                     NNIELLQSILTPSPHPKAMFQTSKAIDELTLIRLNQRKPAFDDIATRLKSDQIDFFAN
                     EISSVLDTVQPVTEVTITRRTEPGRLYEVVRFGLQNAAP"
BASE COUNT          858 a          808 c          927 g          823 t
ORIGIN      
        1 accgcatctg gtcagtcctt gtggatcgtc aggggcaggt ctgggttggt acggaaagcg
       61 gtctggatcg gttggatcgg gcaactggaa cctttacccg ctttcaggaa aaagcggctg
      121 aaaccgggcg cccggttgaa gacaacatca atgttttata tgaagatcgg gcgggtgtgg
      181 tgtgggttgg aacctatgga aacggcctgc tccgcttgga tccaaccaca aaggaagtga
      241 cccgctatct gtcagccgcc tggaatgcga ccagcctggg gagcaacacg gtctttacca
      301 tttatgaaag cacggctggg gccttgtggg ttggcaccaa cggtggcggg ttgagttgcc
      361 tggatcgaac gacgggaaca tttaccagtt acacgatccg tgacggcctt cccagcaatt
      421 cagtgatgag tatgcttgag gacgaagctg gaaatctctg gcttgggaca accctggggt
      481 tatgtcgctt tcacccacca agccgatcat gtcgaaacta tgatagccgc gatggactgc
      541 aaagtaatga attcactcaa aatgcagctt tgaaaaccag tcgtggtgaa atgctttttg
      601 gcggaatcaa tgggtttaac tggttccatc cggaaaaaat tgaagaccgc cagtttatcc
      661 cggcgctgtc actgaccagt tttctcattt atggccgtga acttgaaggg tttaacggca
      721 aagatcttcc accactcgat tacacccaaa actttatcac ctttaaattt gctttcttaa
      781 atgacacggt accggaaaag aatcgctatc agtacaaact tgaagggttt gaccgggatt
      841 gggtcttgtc tggttcacag cggtatgcga gttacacgga tttgaaacct ggcacctacg
      901 tttttcgagt caaaggggcg aactccgatg gggtctggga tcagcaggga attgcactcc
      961 gttttgaggt gattccgccg ccgtggaaaa cctggtgggc ctattcactg tatgtggttg
     1021 ggattgttgg tggttcgtgg ctgggcgttc aattacgcct gcggtcgttg aaacggcgag
     1081 ccatcatttt ggagcaatct gtcattgagc gaacccagga agttgtccag cagaaaaatg
     1141 aactttcgca ccagaacgaa cagattatcc ggcaacggga tgaaatcgag cgcaaaagcg
     1201 ccgaactgga gcgccgttcc cgagagattc aggaaaaaaa cagccagatc attgacagta
     1261 ttctgtatgc cgagcgaatc cagcaggcga ttctggccta ccggaagcgc attggtcagt
     1321 cgctcagcga gtacttcatt ttgtttcgac ccaaggacat tgtttcaggc gacttttact
     1381 ggtttcacta tgttgacggc atcagcatca ttgccgtggt ggactgtacc gggcacggtg
     1441 ttccgggcgc ctttatgtca atgattggca atagtttatt gagtcaaatc gtgattgaaa
     1501 accaggttca cgatcccgcc caaatcctgg aattgatgca tgttggtatt cgcaaagcct
     1561 tgaaacagga cgctgacgac gctgaatcac aggatggcat ggatgtcgcc gtctgtcgga
     1621 ttgatccact ccagcacaca atcatttttg ccggtgcaaa acgcccgttg tactacatca
     1681 acggtggtgg agaattgatc gaagtcaaag gcgaccgcaa atcagtcggc ggcaagcaaa
     1741 aggaaaccca gcgaaaattc accaatcaaa ccattccaat ccaaaaggaa gtcacggtgt
     1801 atctggcttc ggatgggttc agcgatcagg cgggtgaaac atcccggaaa tttggtaccc
     1861 ggcgatttaa agaactcctg caatcaactt cgggctatag cctcgaagtt caataccacg
     1921 ccctcattga agaactcgaa gcccatcaag gcactgaacc ccagcgcgat gacattaccg
     1981 tggtcggagt gcgcgtccag gccgaacgga attattctcc ggaagggtat ttgatgtgaa
     2041 tgtggggctg aagacgtcgg gctgaagaca tcgggctgaa gaagacgggc tgaagaagac
     2101 gggctgaaga catcgggctg aagaagttgg ttttatttca tcccttatcc ttcagccttc
     2161 atcctttcga ttgctccgag cttgatccct catccttcag ccctcagccc caagcccaat
     2221 gtcttcagcc cgatgttttc agcccgagtt cttcagccct ggttttaggg tttggcgatt
     2281 ccccagatgt gaaggctttc gagcactaaa atcgtggccc aaaccacaat gctgattcca
     2341 gcggcagcag cggcaatgtc tttgatgatg cgaatttttt catcttcgcg ctgttcgaca
     2401 aaatcacaca ggacttcaat cgagctgtta aagagttcgg caatgagcat cagcccggtg
     2461 gcgagcaaaa taatggagag atcaacccac tggcggaaga aaaatgcaac gatcaaaacc
     2521 ggcaatgaga gaaccacttt gtacgcgaca ctaaagtcag tcagaactgc gacatagagt
     2581 ccggcaaacg cgactttgat ttttcgaagt gggtgatacc caggcgtgcg aaatttggaa
     2641 ggcatagtgg tggacaagga aactgagatc agtgaacccc gcacttcgtc cagggcagca
     2701 cttcaaaatg aagaaattag aattcagaca acttggccag ggtataccgc aggctcgttc
     2761 tgttgtccat ctggtctggc caaattgttc cgtcagatgg atttttaaac cctacttatg
     2821 gtgcggcatt ttgaagccca aacctcacga cttcatacag ccgcccaggt tcggttcggc
     2881 gggtaatcgt gacttctgtg acgggttgca cagtgtccaa cacgctggaa atttcattcg
     2941 caaaaaagtc aatctgatca cttttgagcc gggtggcgat gtcgtcaaag gctggtttgc
     3001 gctggttcag gcgaatcaga gtcagttcgt caatcgcttt cgaggtttgg aacatcgcct
     3061 ttgggtgtgg cgacggcgtc aggattgact ggagcaactc aatattattt gccagcacca
     3121 gcgcccggtc tttgagtgca taacaccacg tgagtccgag cattggcagg ttcagttgtc
     3181 gaaatggcag accatttatt tcggagctgg tccactctaa cgatgtcgtt ccgatggcgg
     3241 caattgaaaa ctgactttgt gccagtttga tcaatgattg ttcaaggatt gggcggttga
     3301 gccctgacgg agattggagc atcacgagtg tcgctttgcg aaattcagca aaaagctgac
     3361 cggggcgggc ttgcggactt tgaatgacag cgacggtttg gggaccagca ctggac
//
LOCUS       DAOQDO010000414         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20888_length_3415_cov_2.956845, whole genome shotgun sequence.
ACCESSION   DAOQDO010000414 DAOQDO010000000
VERSION     DAOQDO010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20888_length_3415_cov_2.956845"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3324)
                     /locus_tag="PKZ53_07940"
     CDS             complement(<1..3324)
                     /locus_tag="PKZ53_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40405.1"
                     /translation="MLRRSFQSRSNFHRALFIIGLTILFSTLYLKNIPYSKAAFSATQ
                     KSGSGSRVTIRASQLQNKWLSLSNGTTLTTDDPTTDDADEASRNTSPGQPEALASADF
                     NNDGVPDLITVDTLGGAGSIDLWLANPEYRSLSSLQTHRRDEQEPFQTSPFVSRPIRF
                     SVPDRPRFVGAGDFTGDSQADVVVVSRTTNQLLFFIGDGQGNLIAGPVRALPGKASAF
                     TVGEINRADGLPDLALAVDSLSGSEVLVFEGPLGAMAAEPERFVFPDKVQGLAIGQFD
                     RDSAFDLAVAAGDTVSMIWGRDRRLSTDIETQARVESARLDQIPTGQKIKHLASGNFI
                     GNSATELALLFENNQVGLIEPLELRKPLKAGFSKAVWKQSQITNSPIIGAKELVNVKV
                     SALPHADLAIIGEDATLDLLLPTHGTNTKAPGGQLPRFEEVPLPTTSQALAVLPLRLN
                     QDALTDLVVLQQGERRPSTLVTLPQATFTVTSTADASDLNIGDGICDAGSGTCTLRAA
                     LEEANFTAAADLIEFNVGPGIITFNLGGTLSLPEIVNPVTIDGGTVTGQTIMISGSPM
                     QFGSGLEISSGNCTITDLVINDFPMAGIKLRINGNNIVRGCKTGTDETGTSARPNHNG
                     IEITQSSANNTIGGTATGDGNLCSGNSFTGICLDGSGTDGNIIQGNRIGVTAGGTGPL
                     ANAANGILLKLGPTMNTQIGGSVTGASNHISSNSSDGISIDEALGAMIQQCLIGTSND
                     GLSDLGNGGSGVVVSAFGGPRKPTSSVRTHSQARPQPPQPNRKGTFGPLITSGVTIGG
                     TASLSRNIISGNNSDGISLFGDVTGTIIQNSIIGLDSAGTNPIPNTFSGIFQQDAAES
                     TIGGSVVQARNIISGNNDSGIFFAGDGTAASNLVQGNYIGTDITGTTSFTVSGFSNGG
                     VVIEYSSDNQIGGNTSTPGAPPGNVISGNFKGVVFKAPGEASPAINSSNTIRGNLIGT
                     TANGLSALGNQSSGVSITVDTDGTQIGGTTAGDRNVISGNGGYGIYIQDPSVTNTMIQ
                     GNYVGPNINGTAAVGNQMGGVFIFDTDGHTIGGDTAATRNVISGNTGVGITIQLSPSG
                     NQIQGNYIGVTSTGNAALGN"
BASE COUNT          821 a          867 c          866 g          861 t
ORIGIN      
        1 gttcccgagt gctgcatttc cagttgaagt gaccccaatg taattcccct gaatttgatt
       61 acccgaaggg ctaagctgga ttgtaatgcc gaccccggta ttgccagata tcacgttgcg
      121 agttgcggct gtatcgccgc caatggtatg gccatcggta tcgaaaatga agaccccgcc
      181 catctgatta ccgacggcgg cggtaccatt gatattggga ccgacatagt ttccctgaat
      241 catggtgttg gttacagacg gatcctggat ataaatgcca tatccaccat tgcccgagat
      301 cacatttctg tcgccagcgg tggtgcctcc aatttgagtt ccatcagtat caaccgtgat
      361 tgagacgccg cttgactggt ttcccaaggc ggacaggccg ttggcggtgg tgccaattaa
      421 atttcctcga atggtgttgc tactattgat ggccggggac gcttcacctg gcgctttgaa
      481 tacgactccc ttgaaattcc ctgaaattac attgccgggt ggtgctcctg gggtggatgt
      541 attaccgcca atctggttat cagaggaata ttcaatgacg actcctccat ttgaaaaccc
      601 actcactgta aaagaagtgg tcccggtaat atcggtgcca atgtaatttc cctgaaccag
      661 gttactggcc gcggttccat cacccgcgaa aaaaatcccg gagtcattat ttcccgagat
      721 aatgttgcgg gcctgaacca ccgacccgcc gatagtggat tcggcggcat cttgctgaaa
      781 aatcccagaa aacgtgtttg gaattggatt ggtaccagcg ctgtccagtc caataattga
      841 attttggatg attgtgccag tcacatcacc aaaaagtgaa atgccatcac tgttattgcc
      901 ggaaatgata ttccgggaaa gggaagcggt tccaccaatg gtgaccccac tggtgatcaa
      961 cggcccgaat gtccctttcc ggttcggttg tggcggttgt ggtcgagcct gggaatgggt
     1021 ccgaactgat gatgtcggtt ttcttggtcc gccaaaagcc gaaaccacaa caccagaccc
     1081 accattgcct aagtctgaaa gcccatcgtt ggaagtccca atcaaacatt gttgaatcat
     1141 cgcgcccagg gcttcgtcaa tcgaaattcc gtctgatgaa tttgaagaaa tatgatttga
     1201 ggccccggtc actgaacctc cgatttgggt gttcatcgtt gggccaagct tgagtaagat
     1261 gccattcgct gcattggcca gcgggcccgt tcctccggcg gtcactccga ttcgatttcc
     1321 ctggataata tttccatccg tccctgatcc atctaaacaa atcccggtaa atgaatttcc
     1381 ggaacacaga ttcccgtcac ccgtcgccgt tcccccaatt gtgttgttgg ctgaactctg
     1441 ggtaatctca ataccgttgt gatttggccg ggcactggtg cccgtttcat ccgtgccagt
     1501 cttgcatccc cgcacgatgt tattgccgtt gatccgtaat ttgatgcccg ccattggaaa
     1561 gtcattgatg accaggtcgg taatagtgca gtttccagat gaaatttcga gcccgctccc
     1621 aaactgcatc ggcgagccac tgatcatgat ggtttgaccc gtaactgttc ctccatcaat
     1681 ggtgacggga tttacaattt caggaagtga gagcgtccct cccaaattaa acgtgatgat
     1741 gcctggccca acgttgaact caatcaggtc agcggctgcc gtaaagttgg cttcttcaag
     1801 ggcggctcga agcgtacagg taccacttcc agcgtcgcaa atcccatcgc cgatattgag
     1861 gtcgcttgcg tcagcggttg atgtgacggt aaatgtcgct tgcggcaagg tcaccagcgt
     1921 ggaaggccgt cgttcccctt gttgcaaaac caccagatcg gtcaaggcgt cttgatttaa
     1981 cctcagcgga agcaccgcca gcgcctgaga cgtggtgggt aacggcactt cttcaaatcg
     2041 aggaagctga cctccgggcg ctttcgtgtt ggtgccatgc gttggcagca acaaatcaag
     2101 cgtggcatct tccccgatta tcgccagatc ggcatgaggg agggccgaaa ctttgacgtt
     2161 gaccagttct ttggcaccga taattggaga atttgtaatc tggctctgtt tccacacggc
     2221 tttggaaaat ccagctttga gtggttttcg aagttcaagt ggttcaatca atccaacctg
     2281 attattttca aaaagaagcg ccagttcagt tgcagaattt ccgatgaaat ttccagaagc
     2341 gagatgtttg attttctgac ccgtcggaat ttgatcaagc cgggcggatt caaccctggc
     2401 ttgagtttca atgtcagttg acagtctgcg gtcccgcccc caaatcatcg aaacggtatc
     2461 gccagccgcc accgccagat caaaggcgct gtctcgatca aattgcccaa tggcaagacc
     2521 ttggaccttg tctggaaaga caaatcgctc tggttcagcc gccatggcgc caagcggacc
     2581 ttcaaacacc agaacttcgc ttccagacag gctgtcaact gccagtgcca ggtccggaag
     2641 gccatcagcc cgattgatct cacccaccgt aaaggctgaa gccttgcccg gaagtgctct
     2701 gaccggtccg gcaatcaggt ttccctgacc atccccaata aaaaacaaga gttgattggt
     2761 ggttcggctg accacgacca catcggcctg actgtcgccg gtaaaatcac cagcgccgac
     2821 aaaccgtggg cggtcaggga ctgaaaaccg gataggtctg gagacaaagg gtgaggtttg
     2881 aaagggttcc tgttcatccc gtcggtgtgt ttggagcgaa gacaatgacc gatattctgg
     2941 gttcgccagc cagaggtcaa ttgagcccgc gccacccagc gtgtcaaccg tgattaaatc
     3001 tggaacgcca tcattgttga aatcagcgct ggccagggct tcaggctgcc ccggtgatgt
     3061 gtttcgtgag gcttcatccg catcatcagt tgtcgggtca tcggtggtga gtgtggtgcc
     3121 atttgacaat gagagccatt tgttttgaag ctggctggca cggatagtaa ctctcgaccc
     3181 ggaacctgat ttctgagtgg ctgaaaatgc cgcttttgaa taaggaatat tcttgagata
     3241 cagtgttgaa aataaaattg ttaaaccaat gataaataaa gctctatgga aatttgacct
     3301 ggactgaaaa gaacgacgca gcataccaaa tcctcatcaa aaggaatgat gaattatgaa
     3361 tgaactggtt gcgagttttg aaaattgatc taaacctttg ttttgtatat gttag
//
LOCUS       DAOQDO010000415         3406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20987_length_3406_cov_3.186511, whole genome shotgun sequence.
ACCESSION   DAOQDO010000415 DAOQDO010000000
VERSION     DAOQDO010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20987_length_3406_cov_3.186511"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1213
                     /gene="cas8a1"
                     /locus_tag="PKZ53_07945"
     CDS             5..1213
                     /gene="cas8a1"
                     /locus_tag="PKZ53_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated Cas8a1/Cmx1"
                     /protein_id="HNJ40406.1"
                     /translation="MRGTFLQHPGHFKSKTKASYSFQLGESEAEITITYPICHFFVHQ
                     NFAAELCNKKGELRKDAIPVAGWLNPGAVVRHWAFEKETSFEELSTLALVLLFAPLAC
                     QYFLLRSKLRDRRAQYAVVVPEITDLEAFARRRWHFVENFGVRDFHASNLGDAGMRFL
                     VKDQAAAESRMTKAPRCQVITFGTVDWSSKQKSRTQVHCIEASEKVCELYRTASEYFP
                     DRPIKGKEGTFLASSFARELIAENLSQNLPWFAGLSDKVTSGEAFKILSYEKEGLHNM
                     AVDKSVWTEESERLFVQACHEAFRLTYRKIYSRARDIGEVANLERANERFRTELGRCK
                     NADSFRAFITDFWSRAGQIPTLQEHWRELLALTTGQHSWKTARDLALLALASYKGTKS
                     STVDVVEEFE"
     gene            1240..2142
                     /gene="cas7i"
                     /locus_tag="PKZ53_07950"
     CDS             1240..2142
                     /gene="cas7i"
                     /locus_tag="PKZ53_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HNJ40407.1"
                     /translation="MTFHLFGNVLTRYGAAANNRGENEGNITTLQKLLWKGQVHTTVS
                     AEAIRWALRYHWQINGATELTKVNRQWSDADNDNRLLGEFSDENFIDDDVLGFMRAEG
                     AKEEASDEPKTKGKKPKGTTTARRGVLEVTRAVSTTPYSGDITFNAKSGQKGRTSLYG
                     TEVHATRYQYGFAMTPSRLTKPERVLPTIDGLISLGEVAGNHSRFLYDFSPESVVLRW
                     THDFSPRFLYCFEETEDGEIGADDLIARIDSGDLSPDELFIAGSIVGTSTGEQFRALG
                     VKTFPGIKAAVSELKKRIATDVEL"
     gene            2145..2774
                     /gene="cas5"
                     /locus_tag="PKZ53_07955"
     CDS             2145..2774
                     /gene="cas5"
                     /locus_tag="PKZ53_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas5/Cmx5/DevS"
                     /protein_id="HNJ40408.1"
                     /translation="MIVLNLEVPIACFRQSRAREYAETYPVPPPSTIYGLLLSLVGET
                     DRYKHPGTQLALAQISLPKKSTVIRTFRRLKKKEILDSSNARPDYQELLTDIRLLVCV
                     LPGEDQATPTLYQRLKDAFLQPDQVERFGGLSLGESRDLVNSISLLTESQLQLSARWI
                     LQEEDGLLALPVWVDHVGSRGTRWRRYSVQPLSPFSQPPATAWTTISPT"
     repeat_region   2778..3100
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2851..2886
                     /rpt_unit_seq="gcgaaaccatctccgatgccgaaaggcgttgagcac"
     gene            3230..>3406
                     /locus_tag="PKZ53_07960"
     CDS             3230..>3406
                     /locus_tag="PKZ53_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40409.1"
                     /translation="MGLHALSYCERLFYLEEVEEIRPIPNDYPKGGFLLLFDPLDGSS
                     NIDVNISVGTIFSVL"
BASE COUNT          904 a          816 c          835 g          851 t
ORIGIN      
        1 aggattgcgg gggacttttt tgcagcaccc cggtcatttc aaatcgaaaa ccaaagcgtc
       61 atattctttt cagttgggtg aaagtgaggc agaaatcacc atcacttatc cgatttgcca
      121 tttttttgtt catcaaaact ttgccgctga attatgtaat aagaaaggag aattgcgtaa
      181 agatgcaatc cctgttgctg gttggttaaa ccctggagcc gtagttcgtc attgggcatt
      241 tgaaaaagaa acgagttttg aagaactatc cacccttgcc ttggttttac tatttgcccc
      301 cttagcttgt cagtattttt tgcttcgatc aaagctgaga gataggcggg ctcaatatgc
      361 ggtggttgtt ccagaaatca ctgatttaga agcatttgcc cgccgtcgct ggcattttgt
      421 cgaaaatttc ggtgtgcggg atttccatgc ctcaaatctc ggagatgctg gaatgcggtt
      481 tctggtcaaa gatcaggcgg cggctgaatc ccgaatgaca aaagccccac gctgtcaggt
      541 gatcacgttt ggaaccgtgg actggtcatc aaagcaaaaa tcccgaactc aagttcactg
      601 catcgaagcc tctgaaaaag tctgtgaact ctatcggaca gcttctgagt atttcccaga
      661 tcgtcccatc aagggaaaag aaggaacatt tctcgcctca agctttgccc gtgaattgat
      721 tgcggaaaat ctatctcaaa acctgccctg gtttgccggt ctttcagaca aggtgaccag
      781 tggtgaagcc tttaaaattt taagttatga aaaggaggga ctccacaata tggcagttga
      841 taaatctgtg tggacggaag aatccgagcg tttgtttgtt caagcatgtc acgaggcgtt
      901 caggctgact tatcgaaaaa tctacagccg tgcccgagac ataggtgaag ttgccaatct
      961 ggaacgtgcc aatgagcggt tccgaaccga gttgggccgg tgcaaaaacg cggatagctt
     1021 ccgagctttt atcactgatt tctggtcacg tgctggccaa atcccaacct tgcaggagca
     1081 ctggcgtgaa ttattagctc tgacgaccgg gcaacacagt tggaaaacag ccagggattt
     1141 agcccttctg gccctggcca gttacaaggg cacgaagtct tcgacagtag acgttgttga
     1201 agaatttgaa taaccaatca cgatcaaaag gagaccgcaa tgacgtttca tttgtttggc
     1261 aatgttttaa ctcgatatgg agccgctgca aataaccgtg gtgagaatga agggaatatt
     1321 acaaccctac aaaagttgtt atggaaagga caggtacata ccacggtctc agccgaggct
     1381 attcggtggg cacttcgcta tcactggcag atcaatggcg ccaccgaact taccaaagtc
     1441 aaccgccagt ggagtgatgc ggataatgac aatcggctcc ttggcgaatt tagcgacgaa
     1501 aacttcattg acgacgatgt attgggtttt atgcgggcag aaggcgccaa agaagaagca
     1561 agtgacgaac caaaaaccaa aggcaaaaaa ccgaaaggaa caaccactgc ccggcgcggt
     1621 gtcctggaag taacccgagc tgtttcaacg acgccatatt ccggtgatat tactttcaat
     1681 gccaaaagtg gccagaaagg taggacctcg ctctacggaa cagaagttca tgccacacgc
     1741 tatcagtatg gatttgccat gacaccttcg cgattaacga aaccggaacg ggtgttacca
     1801 acgattgacg ggttgatctc gctaggtgaa gtcgcgggaa atcactctcg gtttttgtat
     1861 gacttctcac cagaatcagt cgttttgcgc tggacgcacg atttctctcc ccggtttctg
     1921 tattgttttg aagaaacaga agatggcgag atcggggctg acgatttgat tgctcggatt
     1981 gattcaggcg acctgtcacc cgacgaactt tttattgctg gttcaattgt tggaacctca
     2041 actggtgaac agttcagagc actcggtgtc aaaaccttcc ccggaattaa agccgccgtt
     2101 tctgaactga aaaaacgaat tgcaaccgac gtggagctat agccatgatc gttctgaatc
     2161 ttgaagttcc aatcgcctgt ttccgtcaat caagagcacg tgaatacgct gaaacatacc
     2221 cggtccctcc accttcaacg atttatgggc tgctcctgtc actggttgga gagacagacc
     2281 ggtacaaaca tcctggtact caacttgccc tggctcaaat cagtcttcca aagaaatcaa
     2341 ctgtgattcg aacctttcga cgcttgaaga aaaaggaaat tctggattcc agcaatgctc
     2401 gacccgatta ccaggaactc ctgacggata ttcggcttct ggtctgtgtt ttacccggtg
     2461 aggatcaagc gactccgaca ctgtaccaga ggctcaaaga tgcctttctt caaccggatc
     2521 aggttgaacg atttggtgga ttatcactcg gagagagccg cgatctggtc aattcaatct
     2581 cgctgctcac tgaatctcag ttgcagcttt cagccaggtg gattttacag gaagaagacg
     2641 gcttgctggc tttgccagtt tgggtagatc acgttggttc acgtgggaca cgttggagac
     2701 ggtacagcgt gcaacctttg tcgccttttt cccaaccgcc agccactgcc tggaccacca
     2761 tcagtccaac ctgatttttc tttttttctc tgatgccttc gggcgttgag cacgataaac
     2821 caggataaaa actgatagtt acaagatatt gcgaaaccat ctccgatgcc gaaaggcgtt
     2881 gagcacaact gctggttgaa ttccttccaa tcttcgggga ggcgaaacca tctccgatgc
     2941 cgaaaggcgt tgagcacctg gctaaaggta aggtcacggc tgaagagttt acgcgaaacc
     3001 atctccgatg ccgaaaggcg ttgagcacag cctggatctt gattgtgacg tgcttatcac
     3061 cgaagcgaaa ccatctccga tgccgaaagg cgttgagcac ccgaaagacg ctgctcgcaa
     3121 accagaaaac caacctgaag gttgaactct gaacgggaaa ccaatccaaa aattgaactt
     3181 tgaacaggag gggcgtatga ccgctgaaca actcccgctg tttcgaatca tgggactcca
     3241 cgcgctaagc tattgcgagc gtttatttta tcttgaggaa gtcgaggaga ttcgcccaat
     3301 ccccaacgac tatccgaaag gcggattcct gctcctcttc gatccgctcg atggctcctc
     3361 caacatcgac gtcaacatct cggtgggtac gattttttcg gtactg
//
LOCUS       DAOQDO010000416         3406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_20990_length_3406_cov_2.689943, whole genome shotgun sequence.
ACCESSION   DAOQDO010000416 DAOQDO010000000
VERSION     DAOQDO010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20990_length_3406_cov_2.689943"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKZ53_07965"
     CDS             complement(<1..390)
                     /locus_tag="PKZ53_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40410.1"
                     /translation="MRVSVVCLVGVVCCLLHLWVSTPAKSQKVSQPPARFQPGGVISA
                     LNKPGVHNTLTDISPDGTVLYITYSVPDFRSTVLASSRLVGGNWSVAQPVSFSDQKSD
                     LGASINPSQDRMFFTSQRPVPGKQKSDP"
     gene            complement(440..607)
                     /locus_tag="PKZ53_07970"
     CDS             complement(440..607)
                     /locus_tag="PKZ53_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40411.1"
                     /translation="MTQRTSASVVFVYNTTGRPLPPASLRFLSLPPNNLLLVADQAAI
                     PGNVWVPKLSR"
     gene            complement(614..988)
                     /locus_tag="PKZ53_07975"
     CDS             complement(614..988)
                     /locus_tag="PKZ53_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40412.1"
                     /translation="MSQAAIREANAPSSHHRNRQNSSHRNSEYAENKVSRLHTARAVE
                     QFLCEQYRLVTQYRRERTELLREIRYLNTELQLHNNRQVQDRINTLIERWKNSTRLIT
                     VARQLLLYVLETRKLPEPETDL"
     gene            1539..3107
                     /locus_tag="PKZ53_07980"
     CDS             1539..3107
                     /locus_tag="PKZ53_07980"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015116283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HNJ40413.1"
                     /translation="MFDYIIVGGGSAGCVLAHRLTEDSTLRVLLIEAGGPDKQREIHI
                     PAAFSKIFHSPADWAYLTDPQPELNNRQLYWPRGKVLGGSSAINAMIYSRANRVDHDH
                     WHQLGLNGWGYTNMLSWYKKSEHQERGASEFHGVGGPLNVADLRTVNPLTEAFVEAGV
                     EVGMTRSSDFNGASQEGVGVFQVTQKNGKRCSAATGYLTPILKRPNLTVWTNCLVKRV
                     LFEGTQAIGVEFLRQGKTEQVRANREILLCGGSINSPQTLMISGVGPANHLREFNIPV
                     VLDLPGVGQNLQDHLLLAVTYECKKPVSLAGAESIGNVLQYLLFHKGMLSSNIAEGGG
                     FLKTRSGLPGPDFEYMFGPTFYMNHGFSNPKGHGFSFAIVHQRPLSRGKLSLRSASPE
                     TAPSLQPNYLSDPADQQFFVEAVKIARHLAQAKAFDPFRGKEVWPGADRTRDTAVLEF
                     ARSALETCYHPIGTCKAGTDSMAVVDPTFRVRGIENLRVIDASVIPTIFSGHPNAVVI
                     ALAEMAASYLRSAN"
BASE COUNT          822 a          909 c          847 g          828 t
ORIGIN      
        1 gggatcactc ttctgcttgc ctggaacagg tcgttgcgat gtaaaaaaca tccggtcctg
       61 gctcggattg atcgaagccc caaggtccga tttttgatct gaaaacgaaa ctggctgtgc
      121 tacagaccag ttccctccca ccagacgtga ggaagccagc accgtcgaac gaaaatcagg
      181 cactgagtag gtaatgtaca gcactgttcc gtccggactg atatcggtga gtgtattgtg
      241 gacccccggt ttattcaggg cagagatcac ccctcctggt tggaaccggg caggcggttg
      301 gcttactttt tgacttttgg caggagttga tacccacagg tggagcagac agcacacgac
      361 accgaccaga cacacaacac tgacacgcat tggcaacgat cccctgaaac tggtaagtca
      421 cggtgacccc cgagccacct caacggctca atttgggcac ccagacgttc cctggaatgg
      481 cggcctggtc agcaaccagc aatagattgt ttggtggaag tgagagaaac cgaagggagg
      541 caggcggcaa cggcctcccg gtcgtgttat acacaaagac aacgctggct gacgttcgct
      601 gtgtcaatca cacttacagg tcggtttctg gttcgggcag tttgcgtgtt tccaacacat
      661 acaacagcaa ttgccgggca accgtaatta accgggttga atttttccat cgttcaatca
      721 aggtattaat tcgatcctgg acctggcggt tgttatgcag ttggagttcg gtgttcagat
      781 accggatttc ccgcaacagt tcggtccgtt ctcgacgata ttgcgtcacg aggcggtact
      841 gttcgcacaa aaattgttct accgcacgcg ccgtatgcag ccgagaaact ttgttttctg
      901 catactccga attgcgatgt gaggaatttt gtcgattccg gtgatgcgat gatggagcat
      961 tggcttcacg aatcgcagct tgagacatag gttcttcctt tcaaaattaa gataaagttt
     1021 cgctcatgcc acacctgtct cggtgtgacg gttgttgaat tccccctggt acccggttga
     1081 agggcgcctc cgacgtaata tccaacccct gggcgacagg aaaacttccc tggcctctca
     1141 acacgtggag acaatgggct tttggtgcag caaccaaagc ggagcgtagt ctgatactaa
     1201 cgagtttgct ggcaagcctg aatcagtttt gccgaaactt ttgcgttttc agttgtcgtg
     1261 gattttttga gagatttatg aaagaaggac tatagcagaa aattttgaag cgaagtcaac
     1321 ttttttttcg gagacgggtc agttcgtcca caaaattttc aaatttatca cccatagcag
     1381 cgagaatcat tccacccgcg gtgcaggctc aatcactcaa tttccaacca gccgttcagt
     1441 tctcagaaaa aaacacttta cggcaagcga ccttcgttag acaatgcagg gttcgcaccc
     1501 caaaggacat ctagagtaaa tccataacga ggctttcaat gtttgattac atcattgttg
     1561 gaggcgggtc cgctggttgt gtgctggccc accgtctaac tgaagattcc accctgcgcg
     1621 tgttacttat tgaagcaggt ggcccagata aacaacgcga aattcatatt ccagccgcat
     1681 tttcaaaaat ctttcacagc ccggctgact gggcatattt gactgatccc cagcctgaat
     1741 taaacaaccg tcagttatac tggccacgag gcaaggtcct cggcggctca agcgccatca
     1801 acgccatgat ttacagccgg gccaaccgtg ttgaccatga ccactggcac caacttggct
     1861 taaacggctg gggctatacc aacatgcttt cctggtataa aaaatcagaa caccaggaac
     1921 gtggcgcgtc tgaattccac ggcgtcggcg gcccgctcaa tgtggccgac ctgcgaaccg
     1981 tcaatccgtt aaccgaagcc tttgttgaag ccggggttga agtcggaatg accagaagtt
     2041 ctgattttaa cggcgccagt caggaaggcg tcggtgtttt tcaagtcacc caaaaaaatg
     2101 gcaagcgatg cagcgcggcc accggatatc tgaccccgat tttaaagcgc ccgaatctga
     2161 cggtctggac caactgtctg gtgaaacgtg ttctctttga aggcacccag gccatcggcg
     2221 tcgaattcct gcgtcaaggc aagaccgaac aggtccgtgc caatcgggaa atcctgttgt
     2281 gcggcgggtc aatcaattct ccacaaacct tgatgatttc tggggttgga ccagccaatc
     2341 acctgcggga gtttaacatt ccagtcgtgc ttgaccttcc gggcgttggt caaaacctgc
     2401 aggatcacct gttgctggcc gtcacctatg aatgcaaaaa gccggtctcg ctcgctggcg
     2461 ccgaatctat tggaaatgtg ctccagtacc tgttgtttca caaaggcatg ctcagctcaa
     2521 acattgccga aggcggaggc tttctcaaaa cgcgctctgg cctgcccggt ccagattttg
     2581 agtatatgtt tgggccaacc ttttatatga accacgggtt ttccaatcca aaggggcatg
     2641 ggttttcctt cgcgattgtg catcagcgtc cgttgagtcg tggaaaattg agtttgcgtt
     2701 cagccagtcc ggaaacggca ccctcacttc agccaaacta cctttccgac ccggcagacc
     2761 agcaattctt tgtcgaagca gtcaaaatcg cccgccatct ggcccaggcc aaagcgtttg
     2821 acccgtttcg tggcaaagaa gtctggcctg gtgccgaccg gacccgcgac acggcagtgc
     2881 tggaatttgc tcgctcggcc cttgaaacct gttaccaccc aatcggcacc tgcaaggcgg
     2941 gaaccgactc gatggccgta gttgacccca cgtttcgcgt ccgaggcatt gaaaacctgc
     3001 gggtgattga tgcgtcggtg attccaacca ttttcagcgg ccatccaaac gcggttgtca
     3061 ttgctctggc tgaaatggcg gccagttacc tccgttcggc aaactgataa aatgcctggt
     3121 tcttggttct tggttcttgg ttcttggttc tttttggtgc ttggctcttg gttcttggtt
     3181 cttggaaagg attggttcca aagcaccaag taccaagcac caagcactaa agaccaagca
     3241 ctaaagacca agcactaaag accaagcacc agtcacagct catatcccag ggtaagaacc
     3301 cgcatttccc cgtcttcttc aacaaagtag atttcaaact ctccccgttc aactttgaca
     3361 acctcaagct caaagagtcc ccaatcactc cagtcccggt acattt
//
LOCUS       DAOQDO010000417         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21013_length_3404_cov_3.088086, whole genome shotgun sequence.
ACCESSION   DAOQDO010000417 DAOQDO010000000
VERSION     DAOQDO010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21013_length_3404_cov_3.088086"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..2603
                     /locus_tag="PKZ53_07985"
     CDS             72..2603
                     /locus_tag="PKZ53_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNJ40414.1"
                     /translation="MNNLDVATRNPEPGTRNPGCDLCGLPCDSSRFIRQDTRFCCAGC
                     MNVYSILVESGLCQPGDDFRETELYQQSLRMGLISNGQVPSATSEVKGTVSPETPGQE
                     ILIHVGGMWCASCAWLIEHVVSKEPGVIKAEAFFASDLVKVKFAPQCLPPTRLIERIE
                     QLGYHASEFTDEEGTRDEEKRDLLLRLGLSGFLWLNIMTLSTALYVGYFEIIAESIRL
                     YLPFVLMVLATPVVFYSASPILKLGYQALRNRTLRMESLLGLGILAAYFYSAVQAFQS
                     GIHIYFDTVCAIVTLVLIGKYIERTAKDRTSQAVTLLYRLMPKKVRLYSNGQERFVSI
                     DALRVEDCFLVKAGERIPADGVVIDGHSFADESLLTGESTPVTKHVESPVIAGSLNGS
                     GVLRVRATKVGNDTTLAQIVHCVEQALSGRSPIERQVDRVSQVFIPSVIVVAALTFLI
                     CVWLGHPTGDSLMRAITVLVIACPCALGMATPLAMTTAIGTASQTGILVSDGSVLESV
                     KTIDTIVLDKTGTVTEGQFSLVGFELCAEPFIPTGLDGHRHPPASSHPELFRQVYLPV
                     LASLEAYSEHPLGIALRKYALEQGVTLCEATEIVIHQGQGISGTVDRRQVFLGNRGLL
                     EHLAGPNAPIFESLQTLQWEADGHTVVFFGLDGELCGLLAFGDCLKPDSIEAVRAFQQ
                     QGLTVHLVSGDSQATVRAVGNQLGADQVLAEVSPAEKASYIQTLQDQGRVVCMVGDGI
                     NDAPALAQANLGVALSSGTDIAMQAASVVLISHSLNKILDVFGLSHKTRRVIRQNLFW
                     AFLYNSLGIGLAVTGILNPILAAGAMVLSSLSVITNSLRLRNIGH"
     gene            complement(2870..3196)
                     /locus_tag="PKZ53_07990"
     CDS             complement(2870..3196)
                     /locus_tag="PKZ53_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40415.1"
                     /translation="MDENSTGAGAGADTGRFELLAMKGFLSIKYWLINRYLSIEFLNW
                     QLVIDSFRKRMARSAAAATQANAIRLRRKRQPVAGGEWTKQPVELSSEYGISDFDSTL
                     QENHVT"
     gene            3210..>3404
                     /locus_tag="PKZ53_07995"
     CDS             3210..>3404
                     /locus_tag="PKZ53_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNJ40416.1"
                     /translation="MERERRYWLVKSEPEVFSIQHLNECPERTAGWDGVRNYQARNFL
                     RDDFKLGDQVFFYHSNAEPPG"
BASE COUNT          750 a          826 c          918 g          910 t
ORIGIN      
        1 ggttccgggt tttcaggcag attgttttct tcctacaact ttgtcctcat aaacactgtg
       61 gatacccgtc tatgaacaac cttgatgtgg cgacccggaa cccggaaccc ggaacccgga
      121 accctggctg tgacctctgt ggcctcccct gtgactcatc gcggtttatc cgtcaggaca
      181 cgcgcttttg ctgtgccggg tgtatgaatg tgtacagtat tttggttgaa agcggtcttt
      241 gccagccagg agatgatttt cgggagacag aattgtacca acagagcctt cggatgggcc
      301 tgatttcaaa tgggcaggtt ccgtcagcca catcagaagt caaaggcacc gtttcgcctg
      361 agacacctgg tcaagagatc ctgattcacg tcggaggcat gtggtgtgcc tcctgtgcct
      421 ggttaattga acatgtggtt tccaaagagc caggggtcat caaagccgaa gcgttttttg
      481 cttcagacct ggtgaaagtc aaatttgctc cccagtgttt accgccaaca cggttaattg
      541 aacgcattga acaacttggc taccacgcga gtgaatttac cgatgaggag gggactcgcg
      601 acgaagaaaa acgcgacttg ttgctccgac tcgggctttc cggttttttg tggctcaaca
      661 ttatgacgtt gagcaccgcg ctctatgtgg gatacttcga aatcatcgct gaaagtatcc
      721 gattgtactt accctttgtc ttgatggtcc tggccacgcc cgtggtattt tattcggcca
      781 gtccaatttt aaaactcggg tatcaggcac tgcgcaaccg cacgttgcgg atggaatcgc
      841 tgctcggatt gggaatcctg gccgcttatt tttatagtgc ggtccaggct ttccagagtg
      901 gaattcatat ttattttgac actgtttgtg cgattgtaac gctggtttta atcggaaagt
      961 atattgaacg aaccgccaaa gatcgaactt cgcaggccgt gacattgctc tatcggttga
     1021 tgccgaaaaa ggtccgactt tattccaacg gacaggaacg atttgtttcc attgatgcct
     1081 tgcgagtcga ggattgtttt ttggtcaaag ctggtgagcg gattccagcc gatggtgttg
     1141 tgattgacgg gcattccttt gctgatgaat cactcctgac cggggaatca acgcccgtga
     1201 ccaaacacgt tgaaagtcca gttattgctg gcagtttgaa tggcagtggt gtgctccggg
     1261 tccgagccac caaagtcgga aacgacacca ccctggccca gattgtgcac tgtgtcgaac
     1321 aggcattgag cgggcgttca ccgattgaac gccaggtaga tcgggtgtcg caggtcttta
     1381 ttccgagcgt gattgtggtg gctgccctca cctttttgat atgtgtctgg ttgggtcacc
     1441 cgacggggga ttccctgatg cgggccatta cggtgctggt gattgcctgt ccctgtgcgc
     1501 tcggaatggc cacaccgctc gcgatgacga cggcgattgg cactgcctcg caaactggaa
     1561 ttctggtcag tgacggcagt gtgcttgaat cagtcaagac gattgacacg attgtgttgg
     1621 ataaaaccgg taccgtgacc gagggccagt tctcgctggt cgggtttgaa ctctgcgccg
     1681 aacctttcat tccaactgga ctcgatggac acaggcatcc gccagccagt tcacacccag
     1741 aactgttcag gcaggtgtat cttccagtgc tggcctcgct tgaagcttat tctgagcatc
     1801 cgctggggat tgctctgcgc aaatatgccc ttgaacaggg agttaccctc tgtgaagcga
     1861 ctgaaattgt gatccatcaa gggcagggga tttcaggcac cgtagatcgt cggcaggtgt
     1921 ttttgggaaa tcgtgggtta ctcgaacacc tggccgggcc aaacgctcca atttttgaat
     1981 cgctccagac tctccaatgg gaagcagacg ggcacaccgt cgtgtttttc gggcttgatg
     2041 gagagctttg tggcttgctg gcgtttggtg attgcctgaa accggactcg attgaggcgg
     2101 ttcgggcttt ccagcaacag ggactgacgg ttcaccttgt ttcaggtgat tcacaggcaa
     2161 cggtgcgtgc cgttgggaat cagcttgggg ccgatcaggt tctggccgaa gtttcgccag
     2221 ccgaaaaagc cagctacatc cagacgctcc aggatcaagg gcgggtcgtg tgcatggtcg
     2281 gagacggtat caacgatgct cccgcgctgg cacaggccaa cctgggggtg gccttgagtt
     2341 ccgggacaga tattgccatg caggctgcca gcgttgtttt gatcagccat tcgctcaaca
     2401 aaatcctcga tgtgtttggt ctatcgcaca aaacccggcg ggtgatccgg caaaacctct
     2461 tttgggcctt tttgtataac tcgcttggca ttgggctggc ggtaaccggc attttaaacc
     2521 caattctggc cgccggagcg atggtgctgt cgagcctgtc tgtgattacc aactcgcttc
     2581 ggttgcggaa catcggccac tgaactgaca tcagataagt atcttgccat aaatgttctg
     2641 agatattggg tttggtacgt ggttgatttt tttccgtgtt ttccgtgttt tccgtggtta
     2701 aaatgtctgg aaattttcgg caagatactt aatcggcgga gaatagctga aagcgtgatg
     2761 ccccaggtga taaaactcat ttgaagaaaa aaacaaatcc cccaaagaac cgctttttta
     2821 cgcatggttt gaactcctcc tggaactggt cataacccat tgaaaaaaat cacgtaacat
     2881 ggttctcctg caatgtagaa tcaaaatcac tgattccata ttcactgctc agttcaactg
     2941 gttgctttgt ccattcacca ccagctactg gttgccgttt tcgtcgcaat cgtatggcgt
     3001 ttgcttgcgt tgcggctgca gcacttcgtg ccatcctttt tctgaaagaa tcaatcacga
     3061 gttgccagtt gagaaactca atggaaagat agcgattgat caaccagtat tttatcgaaa
     3121 ggaatccttt catggccagg agttcaaaac gtccggtatc cgctccagca cctgcaccgg
     3181 tagagttttc atccattcag cctgttgcaa ttgagcgaga gcgtcgctat tggctggtaa
     3241 aatcggaacc cgaagtgttt tcaattcagc acttaaatga atgccctgaa cgaacggcag
     3301 gttgggatgg tgtccgtaac tatcaggccc ggaatttttt gcgggatgat ttcaagcttg
     3361 gggatcaggt gtttttttac cacagcaacg ctgagccccc gggg
//
LOCUS       DAOQDO010000418         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21033_length_3402_cov_3.447864, whole genome shotgun sequence.
ACCESSION   DAOQDO010000418 DAOQDO010000000
VERSION     DAOQDO010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21033_length_3402_cov_3.447864"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PKZ53_08000"
     CDS             complement(<1..1105)
                     /locus_tag="PKZ53_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HNJ40417.1"
                     /translation="MQPQPLGAFLSASVQHGPVPLSEAIALILPLFEQLQETHQQGKV
                     APLVGLSHLKISNQKIWFHDFEATAPQLQLEEIRRVERFLRRQEHQTLRTAADEGATT
                     DDEIPPLQPVFLTGYLTWEHQLGHHDPLTDIFSAGLIFASLLCGLDLNEPADVERFTR
                     AHHALTTLTPSLSPALAHLVRALTELSRQRRIRDLQGVISTLKLLAHNPVALDEPAPF
                     LTEAERSQTMSERQAVLLKLRNRLFDLSRRNPLLYFKATARTFNLTVTSMASFHQGDV
                     AHHLPPVTWTGGIVDRLASGREISLLPLVDAAYTDFAQAALDRIRLEDRRLQNEFGFS
                     QLRLVIAFLHWHHLNSGQPEPIISPFLLLPVSLE"
     gene            1554..2039
                     /gene="tssE"
                     /locus_tag="PKZ53_08005"
     CDS             1554..2039
                     /gene="tssE"
                     /locus_tag="PKZ53_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNJ40418.1"
                     /translation="MARPGAQSTFRPSLLDRFRNATSEPQILGTFQGNSIEAIKQSIA
                     SDLQWLLNSRQSPSEQWEGSPNLEASLLTFGLPDFTGMNPNHGVDQERLRSGIESAIR
                     QFEPRLDQVRVFLQAHLQTERSLHFHISALVRADLVNEPVSFETVLNVGTGSYQWEVW
                     K"
     gene            complement(2189..>3402)
                     /gene="tssI"
                     /locus_tag="PKZ53_08010"
     CDS             complement(2189..>3402)
                     /gene="tssI"
                     /locus_tag="PKZ53_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNJ40419.1"
                     /translation="PAEPTGSPEAQRENFWDMQKETDRYTKLRKEEGTDSQVHFATGS
                     GNCRGFVTGHFFTLTEHSRKDFNQKYVLTSLETRAYQNDSHNEPGTFLQNTFRVIKSD
                     IPFRPDRLTPIPVVQGSQTALVVGPPGEEIYTDKYGRIKAQFHWDRYGKHNENSSCWL
                     RVGTLWAGDHWGFIQLPRIGQEVIVDFLEGDPDKPYVVGSFYNSTHLPPYALDSTSTS
                     SKQGNYKTVSTRKSRSTLNGTPENYNEIRFEDLKGAEQLFIHAERDMDVHTKRDRREH
                     TLRDQHFIVERDHVEEVHRNRNSHISQDQTLSVGNTRSATIGTVDVVEAGQEIHLKAG
                     NKIVLEAGSSITLRVGANFINVTSTHIFIMAPAGMVRIQHDGGPESGSGAQSGRKADV
                     ADDGPHFGPKT"
BASE COUNT          823 a          835 c          906 g          838 t
ORIGIN      
        1 tttcaaggga aactgggagc aacaggaatg gagaaataat tggctcgggc tggcccgaat
       61 tgaggtggtg ccagtgtaaa aacgctatga ccagccgtaa ctgactgaat ccaaattcat
      121 tttgcaggcg gcggtcttca aggcggattc ggtccagagc tgcctgggca aaatcagtat
      181 aggcggcgtc caccagtggc agcagggaaa tctcacgccc agaggccagc cggtccacaa
      241 tacctccggt ccaggtcacg ggcggcagat gatgagctac atctccctga tgaaatgatg
      301 ccatggaagt tacagtcaga ttgaaggttc gggcggtagc tttgaaatac agcagcggat
      361 tgcggcgtga aagatcaaat aaccggttgc ggagcttaag caacactgcc tgacgctcac
      421 tcattgtctg tgagcgttca gcttccgtca gaaatggtgc gggttcatca agcgcgaccg
      481 ggttgtgagc cagcagtttc agggtagaaa taaccccttg caggtcccgg atccgccgtt
      541 gtcgggacaa ttcggtgagg gcccgcacga gatgggccag ggctggtgag agggaagggg
      601 tcagggtggt gagggcgtga tgcgccctgg taaagcgttc gacatcggct ggttcgttca
      661 ggtccaatcc acagagaaga ctcgcaaaaa tcaacccggc tgaaaaaata tcagtcaacg
      721 ggtcatggtg accaagctgg tgctcccagg tcagatagcc ggtcaaaaac actggctgaa
      781 gaggtggaat ttcatcatcc gttgtggcgc cttcatcggc ggcggtacgc aacgtttggt
      841 gttcctggcg acgcaaaaag cgttcgaccc ggcggatctc ttcaagctgg agttgcgggg
      901 cagtggcttc aaaatcatga aaccagattt tctggtttga aatcttgaga tgactgagcc
      961 caacgagcgg ggcgaccttg ccttgctgat gcgtctcctg gagttgctca aacaagggca
     1021 gtatcagggc aatggcttct gagagcggaa ccggaccgtg ttgaactgag gctgacagaa
     1081 acgccccaag tggttgtggc tgcataattt gttgaatgtt gaatagagaa tgttgaatag
     1141 agaatgaaga agcaatccaa cgggttaaac cattttggaa taaaagcctc gtattacggg
     1201 aaggctaacc cattgaaaaa actgtgtttc cctgaaccct ggacccgata ccctgaaccc
     1261 taaatggtat taaccctggt tgctccaacc cactgtcctc agccctgaat ggtagaagtt
     1321 tgaagatacg ctcaacggca tttaaccatc atcaagatga attaaatttt aaattaaacc
     1381 tcctggctga agctgaaggc cctggccacg ggttgattgt tcattgacga taaaaacgat
     1441 aaccggcttt ttatcaattg tttaaatgtg ctacccggcc aggttgaatt tctggtacat
     1501 cgtttgcctg agaatcgcca gagcaagttc agattttcat ttggagtatg accatggcac
     1561 gacctggagc gcaatcaact tttcgtccgt cattgctgga tcggtttcgg aacgctactt
     1621 ccgagcctca gatcctgggg acatttcaag gaaattcgat tgaagccatc aaacaatcaa
     1681 ttgccagtga cttgcagtgg ttgttgaact ctcgccaatc tccatctgaa cagtgggaag
     1741 ggtcgccaaa tctcgaagct tcgctcttga cctttggcct gcctgatttt acgggaatga
     1801 accccaacca tggtgtggac caggagcgat tacgttcagg catagagtcc gccattcgtc
     1861 agtttgaacc ccgactggat caggttcggg tgttccttca agcacacctg caaaccgagc
     1921 ggtcgctcca tttccacatc agcgccctgg ttcgggcaga tttagtcaac gaaccggttt
     1981 catttgaaac tgtcctcaac gtcgggaccg ggtcgtatca atgggaggtc tggaaatagc
     2041 ggatcttcca actaccaaag cggtgtcaag ccaccgcact ccacaggcgt caagccgccg
     2101 aaaaggaaag cggtgtcaag ccaccgcact ccacaggcgt caagccgccg agaaggaaag
     2161 cggtgtcaag ccaccgcact ccacaggttt acgttttcgg tccaaagtgt ggcccatcat
     2221 cggccacatc agctttgcga cctgattgag caccagaacc gctttcaggg cccccgtcgt
     2281 gttgaattct gaccattccg gcaggtgcca taataaaaat atgggttgag gtgacgttga
     2341 taaagttcgc accaacgcgc aaggtgattg aactgccagc ttcaagcacg attttattgc
     2401 cggctttgag atggatttcc tggccagctt ccaccacatc cacagtgccg atggtggcgc
     2461 ttcgggtatt tccaaccgac aaggtttgat cctgagaaat atggctgttt cggttgcggt
     2521 ggacttcttc gacgtggtcg cgttcgacaa tgaaatgctg gtcacgcaag gtatgttcac
     2581 gccggtcacg cttggtgtgg acatccatat cgcgctcggc atgtataaac aattgttcgg
     2641 ccccttttaa gtcctcaaat cggatttcgt tataattctc aggtgttccg ttgagcgtac
     2701 ttcggctttt gcgggtgctg acggttttat aattcccctg ctttgaactg gtgctggttg
     2761 aatccagggc gtagggtggc aggtgggtgc tgttatagaa actcccaacc acgtagggtt
     2821 tatccgggtc gccttccaga aaatcaacga tgacttcctg cccaatgcgt ggcaactgga
     2881 taaatcccca gtgatcaccc gcccagagtg tgccaacccg caaccagcac gaactgtttt
     2941 cattgtgttt tccatatcga tcccaatgaa attgcgcttt gatgcgacca tatttgtcgg
     3001 tatagatttc ttcgcctgga gggccaacca ccagggctgt ttgtgatcct tgcaccaccg
     3061 gaatgggcgt taaccggtct ggacggaaag gaatatcact tttgataacc cgaaaggtat
     3121 tttgaagaaa agtacctggc tcgttatgac tgtcattttg ataggctctt gtttcaagcg
     3181 aagtcaaaac atatttctgg ttaaagtctt tccggctatg ctcggtcagg gtaaagaaat
     3241 ggccggtcac aaatccccga cagtttcctg atccggtcgc aaaatgaacc tgactgtcgg
     3301 tgccttcttc tttgcgcaat ttggtatagc ggtcggtttc cttttgcata tcccaaaaat
     3361 tttctcgttg tgcttcggga ctcccagttg gctctgccgg at
//
LOCUS       DAOQDO010000419         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21070_length_3399_cov_3.104665, whole genome shotgun sequence.
ACCESSION   DAOQDO010000419 DAOQDO010000000
VERSION     DAOQDO010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21070_length_3399_cov_3.104665"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKZ53_08015"
     CDS             <1..428
                     /locus_tag="PKZ53_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNJ40420.1"
                     /translation="LEIARRSRGTPRIANRLLRRVRDIAEVLHSGEITETIAREALNR
                     MEVDRFGLDEVDRKMLLTIIEKFDGGPVGVSTISAAIQEEKDAIEEIYEPYLIQIGFL
                     NRTPRGRVVTRAAYEHLRIPYQVTSTVFSPAPLFEKTAE"
     gene            complement(626..1780)
                     /locus_tag="PKZ53_08020"
     CDS             complement(626..1780)
                     /locus_tag="PKZ53_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40421.1"
                     /translation="MAGMKFRRSCDRCNATFFSEDRKARFCPKHQPKQQPTPTLIKSR
                     PPQAPQHSSAAPAPVSSVSPALSPAPHHHPASSHQSRPPFQRSGKFVAGDRPRPGGGG
                     PRNRPSFRDNRKKTPKQPRLPQQSVLTPELRDQIIKAFEVIKKVPGSELPINQTLTEL
                     NLRNIHTQISQQLWVKRSLVAQVIREVRNLVPAVTSVDLTPEQYERATQTYAHYVQNG
                     LRPEGGRRKSIARELGVPLQSVVLAVRKWAQERYLESQTPQPTRRQLFEIEKCYWRHM
                     NEGKTPYELFPEAIAKELGFVTGYQVLRWVDVLHDNTKVLNVSQEPTPEQKEHVITAY
                     HEYLKSEKPPEHALHPTLAKQVGISAKQVHKVLYDYRMAQRDRYLQLVMV"
     gene            complement(1890..2723)
                     /locus_tag="PKZ53_08025"
     CDS             complement(1890..2723)
                     /locus_tag="PKZ53_08025"
                     /inference="COORDINATES: protein motif:HMM:NF025094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNJ40422.1"
                     /translation="MIVACPSCHTKYQLNPAKVVGDWAPLICRHCATRFNAVVEERLQ
                     RVPAEGVVLMAAPPSSPIRDPLVKALREQGLAVKVVEQAAATSHYLGLCAPDIFLTTP
                     FLSDGSAQKPCEQIHRDIHLKGCAIVLLGTHQSDETQRVQFFADFILNTEQPFESVLR
                     DFAVFLKHFRASQQQHGQFLSELRRAERLARTILSDIILYNPEEVEKGIVEGTFNDLL
                     SEELEDARKYYAEQVHPDVLRETDILEDLLKKFMVEQRQKHSVGLTGHSAQSANAESH
                     S"
     gene            2960..3034
                     /locus_tag="PKZ53_08030"
     tRNA            2960..3034
                     /locus_tag="PKZ53_08030"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2992..2994,aa:Cys,seq:gca)
BASE COUNT          832 a          842 c          862 g          863 t
ORIGIN      
        1 cactcgaaat tgcccgtcgt tcacgtggaa cgccgcgtat tgccaaccgg ctcttgcgcc
       61 gggtacgcga tattgccgaa gtcctccaca gcggcgaaat cacagaaacc attgctcgtg
      121 aagcattaaa ccggatggaa gttgaccggt ttggattgga tgaagtagac cgaaaaatgc
      181 tcctgaccat tattgagaaa tttgatggtg gaccagttgg cgtaagtacc atttcggcgg
      241 ctattcagga agaaaaagac gcgattgagg aaatttacga accgtatctg attcaaattg
      301 gctttttaaa tcgcacacca cgcgggcgtg tggtcactcg ggcagcctat gaacatctgc
      361 gaattcccta tcaggtaacc tccacagtct ttagcccggc ccccttgttt gaaaaaacag
      421 ccgaatgaga acttgagaaa gaaacaaaaa ggggcaccgc aaattgcggt gcccctcaaa
      481 aaccagggct gaaaaaaccg ggctcagggc ttggggctga agaaggaggg ctgaagaact
      541 ggttttattt catccttcat ccttcatccc tcatcccttc gattgcccca agccctgagc
      601 ccggtttttt cagcccgaag ctttctcaaa ccatcaccag ttgtaaatac cggtcacgtt
      661 gcgccatacg gtaatcatag agcaccttat ggacctgttt ggcagaaatt cccacctgct
      721 tggccagggt cggatgcagg gcgtgttcag gcggtttttc tgatttgagg tattcgtgat
      781 aggcagtgat gacatgctct ttctgttcag gtgtcggctc ctgactcaca ttgagcactt
      841 tggtattgtc gtgcaaaaca tccacccagc gcagaacctg atagccggtg acaaagccca
      901 gttccttggc aatggcttcg ggaaacagtt catatggcgt tttgccctcg ttcatatgcc
      961 gccagtaaca cttttcaatt tcaaaaagct gccggcgggt cggctgtggg gtttgggatt
     1021 caagatagcg ttcttgcgcc catttcctca cggccagcac cactgattgc aaggggacac
     1081 ctaattcacg ggcaatgctc ttacgacgac caccttcagg gcgaagtcca ttttgaacat
     1141 aatgagcata ggtctgggtg gcccgctcat attgttccgg ggtcagatca actgaggtca
     1201 cagcaggaac cagattacgg acttcacgaa tgacttgcgc caccagtgag cgctttaccc
     1261 ataactgctg tgaaatttgc gtgtggatat tgcgcaaatt caactcagtc aacgtctgat
     1321 tgatgggcaa ttcagatcca ggcacttttt taatgacttc aaaggcttta ataatctgat
     1381 cgcgcagttc aggtgttaaa actgattgtt gcggcaaccg tggctgtttg ggggttttct
     1441 ttcggttgtc gcgaaatgaa gggcgatttc ggggaccgcc gccacctggt cgtgggcgat
     1501 caccagccac aaattttccg gaccgttgaa acggcggacg cgactggtgt gatgaagccg
     1561 gatgatgatg tggcgctgga gataaagctg gggaaacgga tgagacggga gcaggagcag
     1621 ccgacgaatg ctgaggcgcc tgggggggac gggatttaat gagggttggt gtcggttgct
     1681 gtttgggttg gtgcttgggg caaaatcgag cttttcgatc ttctgagaaa aaagtcgcgt
     1741 tgcaacggtc acacgagcga cggaatttca tgcctgccat atggttgact ccactctttc
     1801 agaaaaaccc gttcggaaag ttcgaaacgg gtcgtagatt ttcctcctta gcctgaagag
     1861 tgtcttcaaa tttgactcgt gatgggcagc tacgagtgac tttcagcatt tgccgattga
     1921 gcgctgtgcc cggtcagccc aacgctgtgt ttttggcgtt gttcgaccat aaattttttc
     1981 agcaaatctt cgagtatgtc ggtttcgcgg agtacatcgg gatgaacctg ctcagcataa
     2041 tactttcgag cgtcttcgag ttcttcgctc agcaggtcgt tgaaggtacc ttccacaatt
     2101 cctttttcca cttcttcagg attgtaaagg ataatatctg aaagaatggt gcgtgccaaa
     2161 cgttcagcgc gtcgaagttc acttaaaaat tgtccgtgtt gctgctggct tgcccggaaa
     2221 tgctttaaaa acaccgcaaa gtcccgcagg accgactcaa atggctgttc agtattgagg
     2281 ataaaatcgg caaaaaattg cacgcgctgg gtttcatccg attgatgtgt ccctagcaac
     2341 acaattgcac accctttgag atgaatgtcg cggtgaattt gttcacatgg cttttgagcg
     2401 ctgccatctg acaagaatgg ggtggtgaga aaaatatccg gggcacacaa accaaggtaa
     2461 tgcgacgtcg cggcagcttg ctcaaccact ttgaccgcca gcccctgctc acgaagtgcc
     2521 ttgaccagcg gatcccgaat cggggaggag ggcggtgccg ccatcaacac aacgccttcg
     2581 gcaggcacgc gctgtaaccg ctcctccacg acggcgttga atcgagtggc acaatgtcgg
     2641 caaatgagcg gagcccaatc accaaccact ttggctggat tgagttgata cttggtgtga
     2701 cacgaagggc aggcaacgat catggcgtct caggtgctcc cgcaaaaact gaaccagaaa
     2761 tggaaattgt gatttttgcg gcaaagggga taaaaatgac aggatactca taccctacac
     2821 caagtctgcc tcagaggtaa accgtttgtg tttcggacac ccaactcatc tggtcacagc
     2881 actctccact tgacagaagc cagaccggcg gttagcatcg cacccctact ttcaagtttg
     2941 ttttccaaat caggcgtgag gcggcgtagc caagtggtaa ggcagaggtc tgcaaaacct
     3001 ttattcatcg gttcgattcc gatcgccgcc tccattttta tttcccgcga ccaattcccc
     3061 actcgacaat tttggttctt ttctgaccca actttccgca aggttggtgc cttcatgcgt
     3121 cttgagcagc gatactgggg gtgctattaa agttttttat taagccagat ccttattaaa
     3181 aaataaggga ttatttttta ataagagccc aaaatcagtg tgtttttggc aacttgtctg
     3241 taaaaaccct tcttctcctg actgaactgg ctaccacacc tcttcaaact tccccagttc
     3301 gatgctaatc tgctcggcac ttctcaccca tctgttcaca cctgcgggac tgaaaccacc
     3361 atggcaattg attctgaatt caaaaaaacc ctttgggcc
//
LOCUS       DAOQDO010000420         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21082_length_3398_cov_2.947353, whole genome shotgun sequence.
ACCESSION   DAOQDO010000420 DAOQDO010000000
VERSION     DAOQDO010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21082_length_3398_cov_2.947353"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PKZ53_08035"
     CDS             complement(<1..1339)
                     /locus_tag="PKZ53_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ40423.1"
                     /translation="MVRPRFSLLMKIQLWLLLNLVVLGAVLVGILNLQFQLHPSSPLL
                     GRFETRLEAIGELIRVEVVNTTREQRDVILARYAQAYGIDFYLFDNNGTQLGGIPINL
                     PDDLVFHLHGGGTRPPPPPPRPKGPGQRHPIFKVQTSNPTRYWVGIRVPVVTEGSPEP
                     LRATLLAASYSANGNGLFFDPLPWVYILLAATGFSILFWLPMVRHITKTISQLTKATE
                     QIADGQFEVRVDENRTDELGRLSKAINHLASRLSGYVTGQRRFMGDIAHELCSPIGRI
                     QMALGILDVRIDPKNRMYVEDLEEEVNQMSKLVNELLTFSKAGLKPEAVQLQKVRLRP
                     LVTQITDREAGKGVPLLVEIPDTLLVEANPALLSRAISNIVRNAIRYAGSAGPVRVIT
                     QPEGPHQVRLSVIDSGAGVPESELSRLFDPFYRIERDRARSTGGAGLGLAIVKS"
     gene            complement(1342..2079)
                     /locus_tag="PKZ53_08040"
     CDS             complement(1342..2079)
                     /locus_tag="PKZ53_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ40424.1"
                     /translation="MSNSPDTFAKTKVLMIDDDLKLCRLVRDYLEPLGFEVEGVHTGP
                     EGLERAAAGSFQVVILDVMLPGMDGFEVLKNLRRVSNVPVLMLTALGDEFDRIVGLEM
                     GADDYLPKTFSTRELLARLRAVTRRSVLNQAPPPAAQPDDDQLNIHDLTINLAAHRVT
                     LAGQALTLTPLEFDLLVCLARSADRVLSRDRLLDEINGRNYDVFDRSIDVHISSLRRK
                     LGDDPKNPKYIRTIRAAGYMLVRNSED"
     gene            complement(2976..3290)
                     /locus_tag="PKZ53_08045"
     CDS             complement(2976..3290)
                     /locus_tag="PKZ53_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40425.1"
                     /translation="MRRAQPNECLIHLYPILLAELSEVDLKKLFPVGLSEAEPFTEPE
                     PSVGGLVQLENGQYLGIVFGLQTQRLAILKPEGQPTRETLTYLFQEVPELSNFTIWKE
                     KV"
BASE COUNT          853 a          837 c          881 g          827 t
ORIGIN      
        1 aggatttcac aattgccagt ccgagcccgg ccccgccagt tgaacgcgcc cgatctcgct
       61 caatgcgata aaatggatca aacagtcggg aaagttctga ttcggggaca ccagcgccag
      121 aatcaatcac actgagccga acctgatgtg gtccctcggg ttgagtaata acccggactg
      181 gaccggcgct gccggcatag cggatggcat tgcgcacaat atttgaaatc gcccgcgaaa
      241 gcagtgccgg gttggcttcg accagcagcg tgtcggggat ttcaaccagc agcggaacgc
      301 ctttaccagc ttcccggtca gtaatttgag tgacaagtgg gcgcagacgg actttctgga
      361 gctggacggc ctcaggcttg agcccggctt tggaaaatgt cagcagttcg ttgaccagtt
      421 tggacatttg attgacttct tcttcgaggt cttcgacata catccggttt ttcgggtcaa
      481 ttcgcacatc cagaatcccc agggccattt gaattcgacc aatcggcgag cacaattcgt
      541 gggcaatatc acccataaac cggcgctgac cggtgacata cccagacaat cggctggcca
      601 gatggttgat ggctttgctg agtcggccaa gctcatcggt tcgattttcg tcaacgcgaa
      661 cttcaaattg accgtctgca atttgttcgg tggctttcgt gagttgagaa atggttttgg
      721 tgatatggcg caccattgga agccaaaaca ggattgaaaa cccggttgcc gccagcaaga
      781 tataaaccca tgggagcggg tcgaaaaata gcccgtttcc gttggctgaa tacgaggcag
      841 ccagcagcgt ggcgcgcaac ggttcgggtg agccttcggt taccactggc actcgaatcc
      901 caacccaata gcgggtgggg tttgaggtct ggactttgaa gattggatgg cgttgccccg
      961 gccctttcgg acgtggcggc ggtggcggtg gtcgggttcc tccaccgtgc agatgaaaca
     1021 ccaggtcatc aggcagattg atcgggatgc cgccgagctg ggtgccattg ttgtcaaaca
     1081 ggtaaaaatc aattccgtag gcctgtgcat agcgggccag aatcacatca cgctgctcgc
     1141 gagtggtatt gacgacttcc acccggatga gttctccgat ggcttcgagc cgggtttcaa
     1201 accggcccag taagggcgac gacggatgta actgaaactg tagattgagg atcccaacca
     1261 gcacggcgcc cagcaccacc agattgagca agagccagag ttgaattttc atcaggagtg
     1321 aaaatcgggg gcgaaccatc gtcaatcctc actgtttcga accagcatat atccagccgc
     1381 acggatggtg cggatgtact ttggattttt tgggtcatcg ccaagtttgc gccggagcga
     1441 ggaaatatga acatcaattg accggtcaaa cacgtcatag ttgcgaccat tgatttcatc
     1501 cagcaggcga tcccggctca acacccggtc tgcggatcgt gccaggcaga ccagtaaatc
     1561 aaattcaagt ggcgttaagg tcagtgcctg accagccagc gtgacccgat gcgcggccag
     1621 gttgattgtc aaatcgtgaa tgttcaattg gtcgtcgtca ggttgagctg ctggcggagg
     1681 cgcttgattg agaaccgacc gacgggtgac agcacgcaat cgggccagca gttcgcgggt
     1741 cgaaaaagtt ttgggcaaat aatcatcagc gcccatttcc aggccgacaa tccggtcaaa
     1801 ttcgtctccg agcgcggtga gcatcagcac cggcacgttt gacacccggc gcagattttt
     1861 gagaacttca aaaccgtcca ttcccggcaa catgacatcg agaatcacca cctgaaaact
     1921 tccggcagcg gcacgttcca gaccttccgg gccggtatga acgccctcga cttcaaagcc
     1981 aagtggttcg agataatctc tgaccagccg gcagagcttg aggtcgtcat caatcatcag
     2041 aactttggtt ttggcaaagg tatcaggtga gttggacata gggctcgggt tctggatttt
     2101 tgccgccgaa gcggtagttg aatggaagca ggtcgggtac ggactgacac aatgacgggg
     2161 taacagggta gcaggaggga taaggtgaat ttctcatctc ttcagaaatc cgactcctgt
     2221 tttcaactat agctggtgaa attttaaaaa tcagccgcct ttacaatccc ttaacaaaat
     2281 tgctgatctt tcaaatatac ccttaacaat cgtttgctaa ggtcatgact gaaaaacgat
     2341 gccctggtga tttcaggttt tcggatcaaa gaagttgact cgtgagacgt gcgcaatagc
     2401 ctgaaatcac caatttgggc cctttgtcgg catcagtttg ttggaattac ctgctatgca
     2461 ggttgccagc ctttgagttt ctccatacta tctctcttcc cctcactcca tatccagttc
     2521 aagcttgaac ttgccgacgc aatgaatcca accattgacg aacttcagga cgcaaatcgt
     2581 tttgaactga tcgcaattat tccatacaaa cgcgcaatgg caggcacatt gggttaacac
     2641 cggctccaga aagcctggtt cattaaaatt gatggattca cggcttgttc gattcgaagg
     2701 ttaaatctct ggtttgggcc cattgactta cctgcaggta tagttcataa aaggcacagc
     2761 ttgtttcaca attcgcttca actaaactga gtaaattgtt taagcacatc agatccaggt
     2821 atggatcatc taacagaagg agccattgct caatcagatt ctgacccaat tggctgagtt
     2881 gtggaggcat acaaaattta tcctggcgaa aataacgaag catcttttca gcaaatagcc
     2941 ggtacctggc attttgatca atcatataac tcaggctaca ctttttcctt ccagatggtg
     3001 aaatttgaaa gctctggcac ttcttgaaaa agataggtca gcgtttcacg ggtaggttga
     3061 ccttcgggtt ttaaaattgc aagacgttgg gtttgaagtc caaacacaat tccgaggtac
     3121 tggccattct caagctgaac cagccctccc acagatggct caggctcggt gaagggttcg
     3181 gcctcactca acccaaccgg gaataacttc ttgaggtcca cctcgctcag ttcagccagg
     3241 agaatcggat aaagatgaat caggcattca tttggttgag cacgacgcat agtgttggtg
     3301 tccttgtgtg gtgtccgttt acagagtatc aagctgaacc tcgaaagaga attctatgag
     3361 aaaaactaca attttagctg tttttttgtt ttgcttag
//
LOCUS       DAOQDO010000421         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21093_length_3397_cov_2.543686, whole genome shotgun sequence.
ACCESSION   DAOQDO010000421 DAOQDO010000000
VERSION     DAOQDO010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21093_length_3397_cov_2.543686"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>3397)
                     /locus_tag="PKZ53_08050"
     CDS             complement(32..>3397)
                     /locus_tag="PKZ53_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40426.1"
                     /translation="GTYPLQPVLFLEFDQQIDPTKVLPFLSLEVGTQRFPVKLAAPDQ
                     IGEPDQPLSKLLKKAKAGRWILFQPVNPLPNDAEVTVRLTSGTPSAEGSLVTTTDLTF
                     SFHTLGPLRLVSYPCMNRKECLPDNWLFFTFSNELNKETFVPEMVTVVPSPADLDSSV
                     SNTNLILMGTFAEDTTYTVQFSGALSDTSGQKLGQTVEVTLKFGHFGPFLSTNRKEMV
                     ILDPAGERVFELTSYRVPKLRVQLFRVMPEDWSQFVVYRSSQIEQKKNAKSLRPPGQL
                     VSDEMFDIPESNMGRVKTTRIDLSPALTDGLGHVVLMVDPIGVERTSRNYTEILWIQS
                     TNLAADIVADQDGLLVWANTLAEGKPVVGGEVTLFPAGNSSQTDHQGLAFFPYSTEWE
                     RNKQLLIVRKDTDSALLPGNYTWWSLNAYSPNANRQDQLRWFVFDDRKLYQPGEVLKL
                     KGWIRQLETKKGGDLISGKWFRELVAYQVHDAAGRSLAKGALRLNVFGGFDGSISIPT
                     NADLGEASVSFTLRGTIAAKLGNLTYRHPFDIYQFRRPEYELKVTTSDGPHVIGTSAL
                     ATVFAGYFAGGPLKKAETRWEFSLIEGQYQPPHHDDFVFGDDRGNPWRLYNPIGSKHT
                     FTGLTDADGRHTVEIQLDELTQPRPVVITAEATMSDVNRQTQSSSTKILVHPSHLCVG
                     LKSHQQFIFSGETFEIETIVTDLDGQVQAGKSVKFDVVRLERRPTKYGYAIVEVDPQT
                     ETVVTGSEPFGWKFSPTTGGLYKLIASVWDDHKRLNQTQISLWVAGAMGGMNPEFSEE
                     QAELIANQTIYEPGETAEILLQAPFFPAEGLLTVQQSGVIHHERFTLDQPARKFLIPI
                     TDHHIPNITVQVNLVGETFRRDGDANELTDAPKQPAYASGMLEFSVSAKLRRLTVTAT
                     PKEEKVHPASKTSITLAVTDASGAPVQQTECVVVVVDEAILALSMRPDVDPSEWFYQK
                     REAGTVFDHTRDNLVLAPLSGNLEMTDQLSRRQGLAAAGAAYDGPPIREESTYSSMPI
                     ELNPASLSETRFGLFQQLLSASPEGGQDPIRLRTNFSPLAAFLPSVVTDKQGQATVDI
                     ELPDNLTRYRILVYAAAGDNLFGKGESLRCQII"
BASE COUNT          799 a          867 c          896 g          835 t
ORIGIN      
        1 gccgccgcat acaccagaat tcgataccgc gtcagattat ctggcagcgc aatgattcgc
       61 cttttccaaa cagattgtct cccgccgccg catacaccag aattcgatac cgcgtcagat
      121 tatctggcag ctcaatgtca accgtcgcct ggccctgttt gtcagtcacc accgaaggca
      181 aaaacgccgc cagcggcgaa aaattggttc gcaaccggat tgggtcctgg ccaccttcgg
      241 gtgaagctga aagaagttgc tgaaagagcc cgaatctggt ttctgagagt gacgccgggt
      301 tgagttcaat cggcattgag gaataggtcg attcttcccg gatgggtggt ccatcgtagg
      361 cggcaccagc ggcggcaaga ccttgccgac gggataactg gtcagtcatt tccagattac
      421 cggagagtgg tgccagtacc agattgtctc gggtgtggtc gaaaacggtt ccggcttccc
      481 gcttttgata gaaccactcc gatgggtcaa catcaggtcg catcgaaagc gccagaatgg
      541 cttcatccac taccaccacc acgcactcag tctgttgaac cggtgctccg ctggcatcag
      601 tcaccgcaag cgtgatcgaa gttttactgg ccggatgtac tttctcttct ttgggcgttg
      661 cggtaacggt gagacggcgg agtttggctg aaaccgaaaa ttctagcatc ccgctcgcgt
      721 aggccggctg cttcggagcg tcggtcagtt cgttggcgtc tccatcccgg cggaaagttt
      781 ccccgaccag attgacctgg accgtgatat ttggaatgtg atggtcagta atcggaatca
      841 aaaatttgcg ggcgggctgg tcaagtgtaa atcgttcgtg atgaatgact cctgactgct
      901 gcaccgtcag cagcccttcg gctgggaaga atggcgcttg aagcagaatt tcagccgttt
      961 cacccggttc ataaatagtt tggttcgcaa tcagttcagc ctgttcttct gaaaattctg
     1021 gattcatgcc gcccattgcg cccgcaaccc agagggaaat ttgggtttga ttgagtcgtt
     1081 tgtgatcgtc ccaaaccgaa gcgatcagct tgtataatcc gccagttgtc ggtgaaaact
     1141 tccacccaaa cggctctgat ccagtaacca cggtttcagt ttgcggatca acttcaacaa
     1201 tggcgtatcc gtatttcgta ggccgccttt ccagtcgaac aacgtcaaac ttgactgatt
     1261 ttcctgcctg tacctggcca tcaaggtcag tcacgatggt ttcaatttca aatgtttcgc
     1321 cagaaaaaat gaactgctga tggctcttga gaccgacgca gaggtgagat gggtgcacca
     1381 gtattttggt tgaacttgat tgtgtttggc ggttgacatc gctcatggtg gcttcggctg
     1441 taatcacgac cgggcgcggt tgggtcaact cgtcaagctg gatctcgacg gtgtggcgac
     1501 catcggcatc agttaatcca gtgaaagtat gtttcgaacc aatcggatta taaagtctcc
     1561 acgggtttcc tcgatcatcc ccaaagacaa aatcgtcgtg atgtggtggt tgatactggc
     1621 cttcaatcag ggaaaattcc catcgggttt cagctttttt gagcggccct ccggcaaagt
     1681 aaccggcaaa aaccgttgcc agtgccgaag ttccaatcac gtgcgggcca tcgctggtgg
     1741 tcactttaag ttcgtattcc ggacgccgaa attggtaaat gtcaaaggga tgccggtagg
     1801 taagattgcc cagtttggcg gcgattgtgc ctcgcaaggt aaaggaaacg ctggcctcac
     1861 ccagatcggc attggttgga attgaaatcg agccgtcaaa tccaccaaat acattgagcc
     1921 ggagggcgcc tttggccagg gatcgtccag ccgcatcatg aacctgatag gccacgagtt
     1981 cgcgaaacca cttacccgat atcagatcgc cacctttttt cgtttcaagc tgccggatcc
     2041 agcctttcaa cttgagcact tcgcccggct gatagagctt tcggtcatca aagacaaacc
     2101 atctgagttg atcttgacgg ttggcattgg gtgaataggc attcagactc caccaggtgt
     2161 agtttcccgg taacaaagct gaatcggtgt ccttgcgaac aatcagtaat tgcttgttcc
     2221 tttcccattc agttgaatag ggaaagaacg ccagtccctg gtgatctgtc tggctggaat
     2281 tcccggctgg aaacaacgtt acctcacccc caacaacagg tttcccctca gccagcgtat
     2341 tcgcccagac cagcaagcca tcctgatcgg ccacaatgtc agccgccaaa ttggttgatt
     2401 ggatccagag aatttcggtg taatttcggc tggttcgttc aactccaatt ggatcaacca
     2461 tcaggaccac atgtcccaaa ccatcagtga gcgccgggct gaggtcaatt cgggttgttt
     2521 tcacacgccc catattggac tctggaatat cgaacatttc atcgctgacg agctgaccgg
     2581 gtggacgcag cgattttgca tttttcttct gttcgatttg agatgagcga tagacgacaa
     2641 attgcgacca atcttcaggc atcacgcgaa acagttgtac ccggagtttt ggaactcgat
     2701 atgaggtgag ttcaaacacc cgctccccag ccggatccaa aatgaccatc tccttgcggt
     2761 ttgtgctcag aaacggacca aaatgaccaa atttcagtgt cacttcgact gtttggccaa
     2821 gtttttgccc ggatgtatcg ctcaacgcac cagaaaattg aaccgtgtag gtggtatctt
     2881 cggcaaacgt acccatcaga atcaggttgg tgttgctcac tgatgagtcc aaatcagccg
     2941 gcgacggtac aacggtgacc atttcaggca caaaggtttc tttgtttaat tcattggaaa
     3001 aggtgaagaa cagccagttg tccggtaaac actccttgcg gttcatacag gggtatgaaa
     3061 ccagccgcaa cggccccaga gtgtgaaatg agaaggtcaa atcggtggtt gtcaccagtg
     3121 agccttctgc ggacggggtg ccgcttgtga gccggaccgt gacttcagca tcatttggga
     3181 gcgggttcac tggctgaaat agaatccagc gtccagcttt ggctttcttc agcagcttgg
     3241 aaagcggctg gtcgggttcc ccaatttgat ccggagcggc aagtttgacc gggaagcgct
     3301 gggtgccgac ttcgagcgaa agaaagggaa ggacctttgt cggatcaatt tgctggtcaa
     3361 attcaagaaa gagcactggc tggagcggat acgtgcc
//
LOCUS       DAOQDO010000422         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21103_length_3396_cov_2.987130, whole genome shotgun sequence.
ACCESSION   DAOQDO010000422 DAOQDO010000000
VERSION     DAOQDO010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21103_length_3396_cov_2.987130"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKZ53_08055"
     CDS             complement(<1..469)
                     /locus_tag="PKZ53_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ40427.1"
                     /translation="MNRRQFLLSAGMSLAMLSVFSPSLLARQAKGEISGKVISRDTDK
                     PVVDATVVARNNGTGKEVEVSTGADGKFRFTGLRAGTYLIGVQAAGFESRRLNTMITL
                     AADEVVTLKDAIKLLTEQAVSVIRGAVFTEQGLSLALAKVVIERIGADGKSTAG"
     gene            complement(466..>3396)
                     /locus_tag="PKZ53_08060"
     CDS             complement(466..>3396)
                     /locus_tag="PKZ53_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ40428.1"
                     /translation="GGVARFEKNTWTIFDIDRAGLPNNVVLSLLETRLQGRTRTLWIG
                     TNGGGLARFEPGQWSVLDRASGLPNNLVLALLETSHHQSHAFWIGTRGGLVRFENGTR
                     TLFDETSGLPNNIVTCLLETISPTGQPVIWAGTNGGGLARFENGRWTQITAASGLPSD
                     VVLSLYATRTADGGSVVWVGTDGGGVGRYDGKTWQTIDSKSGLPNNVIFGFLETTAPD
                     GKRTLWIGTNGGLVHNRNGQWETIGINAGLPNDGINSLAEIRLSDGTPTLWVGTYGGA
                     AWCRLDKPTDGWTVLSDTSRPALPNNTIYHLRQDIRGRIYLFTNKGIARLTHRAKPAE
                     DGTVFDLATFTTEDGLPSNECNLGASLIDSAGRLWAGTVEGAACFDLSQEFEDLTPKP
                     LCIERLSINGQLHSGSPGETTLRYNQNNLLFEFTLLNYFRTGDTRYRFQLEGFDATES
                     AWTPEPKKEYTNLGPGPYTFKVWGKDYAGNISGPVMVAIRIAPAPWRTWWAYLLYGVT
                     GSLLLFGGVRWRLQTLEHRNQVLEARVQERTNQLAQKNEELNQNNVALDRKTVELIEK
                     NTELDRLIGELQQAKAVVEAKNQELLHKNEELVRSKEELVQSNQRADRIFSALADVLP
                     GTVLDGKYRLESKIGAGGFGTVFRATHLALQKPIAVKVFRPTAGNATTESLNRFFREG
                     ISSCRVNHPNAVTVLDSGISETGIAYLVMELLVGRSLTQELKLFGRCSPLRCAQILVP
                     VCQALAKAHASGLIHRDIKPDNIFLHQTDQGEVVKVVDFGIAKLMGETTDQIEMHSLT
                     EAGKIIGTPTYMAPERFADKPYDGKTDVYSVGVVAYQMLSGDVPFRATGGEVISIILA
                     HLREMPVSLRRALPEIPEVVEALVFSALAKDPQARPTAEDLARELAAAVDLKAADAPS
                     RATMLLTSEIGEASLVEVDTETIASIVPSTGSATRQLNTGGSSPQHTDPLETAKTLTQ
                     PKIL"
BASE COUNT          791 a          963 c          839 g          803 t
ORIGIN      
        1 tgccggcggt tgatttccca tccgccccga tgcgttcaat cacaactttg gccagcgcca
       61 gcgaaagacc ctgttcggta aataccgcac cgcgaatcac tgataccgcc tgttcagtca
      121 ataacttaat agcatctttg agggtcacaa cttcatctgc cgccagcgtg atcatggtgt
      181 tgagtcggcg tgattcaaat ccggcagcct ggactccgat caggtacgtg ccggcgcgca
      241 gcccggtaaa ccgaaatttt ccgtctgccc ccgtggaaac ttcaacctct ttccccgtgc
      301 cattattgcg ggccaccacc gtggcatcca caactggttt gtcagtgtct cgggagatca
      361 ctttaccgga aatttcacct ttggcctgac gtgccagtag cgatggagaa aaaacggata
      421 acatagccag ggacatcccg gcactcaata aaaactgtct tcggttcata aaattttggg
      481 ttgagtcagg gttttcgctg tttccagcgg atcagtatgt tgcggcgatg acccaccagt
      541 attcaactgg cgcgtggcac tccctgtgga aggcacaatt gaggcaatgg tttcagtgtc
      601 tacttcaacc agactggctt cgccgatttc actcgtcagc agcatagttg cccgagatgg
      661 ggcatcggct gctttgagat ccacagccgc cgccagttca cgagccagat cttcagcggt
      721 tggacgcgcc tgtggatctt ttgccagggc tgaaaagacc aatgcctcaa caacttctgg
      781 gatttcagga agcgcccgtc ttagagacac cggcatttca cgaagatggg ccagaataat
      841 cgagatgact tccccacccg ttgcccgaaa aggaacatcg ccggaaagca tctgataggc
      901 gaccacaccc acgctgtaga catccgtttt cccatcatag ggtttatcag caaagcgttc
      961 tggcgccata taagtcggcg ttccgatgat tttcccggct tcggtcagac tgtgcatctc
     1021 gatttgatcg gtggtctctc ccatcagttt ggcaattccg aaatcaacca ctttgaccac
     1081 ttcgccctga tcagtctggt gtaaaaaaat attatccggc ttgatgtcgc gatgaatgag
     1141 acctgaggcg tgggctttgg ccagtgcctg gcataccggc accaaaattt gcgcacatcg
     1201 gagcggcgaa caccgaccaa agagcttgag ttcctgggtc aacgagcgtc caaccagtaa
     1261 ttccattacc agataggcaa ttccggtttc agaaattcca gagtccagca ccgtaaccgc
     1321 gttgggatga ttcacccggc aactggaaat accttcgcga aagaaccggt tgagactttc
     1381 ggtcgtggca ttgcccgctg ttggtcgaaa cactttgacc gcaattggtt tttgcagtgc
     1441 cagatgggta gcacgaaaga ctgttccaaa cccaccagcg ccgattttgc tctccaaccg
     1501 gtattttcca tccagcacgg ttcctggcag cacatcagcc agcgccgaaa aaatccggtc
     1561 ggcgcgttga ttggattgca ccagttcttc ttttgagcga accagttctt cgtttttgtg
     1621 cagcaattcc tggtttttcg cttcaaccac cgcttttgcc tgttgaagtt caccaattaa
     1681 ccgatccagt tcggtgtttt tttcaatcaa ttcaacggtt ttgcgatcaa gggcaacatt
     1741 gttctgattt agttcttcat ttttctgcgc aagctggttg gtgcgttcct gaaccctggc
     1801 ttcaagcacc tgattgcggt gctcaagcgt ttgaagccgc caccgaacac caccaaaaag
     1861 caacaagctg ccggttacgc catacagcag atacgcccac cacgtccgcc acggtgcagg
     1921 cgcaatccga atcgccacca tcacgggtcc agagatgttc ccggcgtaat cttttcccca
     1981 aactttaaag gtgtacggcc ctggcccaag gttggtgtat tccttttttg gttcgggtgt
     2041 ccaggcagat tcagttgcgt caaatccttc caactgaaag cgatagcggg tatcacctgt
     2101 ccgaaagtaa ttgagcaggg taaattcaaa gagcagattg ttctggttat agcgcagtgt
     2161 ggtttcgccg ggtgaaccag agtgcaactg gccgttgatt gaaaggcgtt cgatacaaag
     2221 cggcttgggc gtcagatctt caaattcctg agacaaatca aaacaagccg cgccttcaac
     2281 cgttccagcc cacaaccgcc cggcactatc aatcaacgag gcgccaagat tgcactcgtt
     2341 gctgggcaga ccatcttcgg tggtaaatgt cgccaaatca aacaccgtgc cgtcttcggc
     2401 gggtttcgcc cggtgggtta atcgggcaat acctttgttg gtaaagagat aaatgcgccc
     2461 ccggatgtcc tggcggaggt ggtaaattgt gttattgggc aaagccggac ggctggtatc
     2521 agaaagcaca gtccacccat ctgtcggttt gtccaaccgg caccaggcag cgccaccata
     2581 cgtgccgacc cagagcgtcg gggttccatc tgacagacgg atttcagcca gtgaattaat
     2641 cccatcatta ggcaaaccgg cgttgatccc aatggtttcc cactgaccat ttcggttgtg
     2701 gaccagccca ccattggtgc caatccacag cgttcgcttg ccatctggtg ccgtggtttc
     2761 aagaaatcca aaaatgacgt tattgggcaa accacttttt gaatcaatgg tttgccaggt
     2821 tttgccatca tagcggccaa ctccaccgcc gtcggtgcca acccacacca ccgagccgcc
     2881 gtcagccgtc ctggttgcat acaaactgag caccacatca cttggaagcc ccgaggccgc
     2941 cgtgatctga gtccagcgac cattttcaaa ccgggcaagc ccgccaccat tggtcccagc
     3001 ccaaatcacg ggctgcccgg tgggagagat ggtttcaagc aagcaggtca caatgttatt
     3061 tggcaaacct gacgtttcgt caaacaacgt ccgggtacca ttttcaaagc gaaccagtcc
     3121 gccgcgagtc ccgatccaaa aggcatgtga ttgatggtga ctggtttcaa gcaatgccag
     3181 caccagattg tttggcaagc cgctggctcg atccaggacg ctccattgac ccggctcaaa
     3241 ccgggcgagc ccgccgccgt tggtaccgat ccacaacgta cgcgttcggc cctgaagccg
     3301 tgtttcaagc aaactcagca cgacattgtt gggtaacccg gcacgatcaa tatcaaaaat
     3361 cgtccaggta tttttttcaa atcgcgccac acctcc
//
LOCUS       DAOQDO010000423         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21113_length_3395_cov_3.104192, whole genome shotgun sequence.
ACCESSION   DAOQDO010000423 DAOQDO010000000
VERSION     DAOQDO010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21113_length_3395_cov_3.104192"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PKZ53_08065"
     CDS             complement(<1..1185)
                     /locus_tag="PKZ53_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40429.1"
                     /translation="MCSPFWLQKKVNVCFMMRCALGVMLLLIFSALPTQAQSAEDVID
                     VVDYKIDAELIPETQTLAAKAIVTLKVTRQVQSVTLELNGSLEVTKVLGPNNEPLQFV
                     QDRLQDLSVKVALGQSPVVGQPFTLTFEYAGQLLSSEGGVLALKRLAYVGKDGCYLLY
                     AARWFPFHAYASDVATYTINLVTPSEFKVVAFGEHTEQPFVPPPPVKPVTGKQTESAK
                     PPTHRQPAGSRRKPAQPAETEKATTEASQLPTKPLPTGSGNRTRHIFVVTQPVLPGTI
                     AAGEYAVRSVQRNGFDVDFYFKIGNESSAERYAEIMAEAGSFLNEKFGPYAFGTKLQV
                     AEIDNESLESYTAAGVTLLARRLLDNPKELPRETLIREVAYQWWGQAVGLKSFDDIWI
                     SQG"
     gene            complement(1418..2470)
                     /locus_tag="PKZ53_08070"
     CDS             complement(1418..2470)
                     /locus_tag="PKZ53_08070"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNJ40430.1"
                     /translation="MDRTQRLRGLMLGTAVGDALGLPAEGLSRQRIRKWYGGTWRHRF
                     CFGWGMVSDDTDHTVFVAQSLLAHPDSVERFTSRLGWCLRGWVASLPAGIGVATLRAT
                     LKLWIGFPPSRSGVFSAGNGPAMRSAPIGAFFAENPELRTTFVTASTRLTHTDPKAVI
                     GARAVADIAAWVMRENLVSRPSKEDFLALLHQVGTDADWQELLQKVSTAIDQELSVET
                     FADRLGLSRGVSGYMFHTVPVALYAWYRHFGDFEATLKSVLDLGGDTDTVAAITGALA
                     GTVVGESGIPLPWRTGIWDWPRGRRILETIADRLAAPGPSAVPIRYFWPALPARNLVF
                     LLIVLGHGFRRLAPPY"
     gene            2550..3206
                     /gene="udk"
                     /locus_tag="PKZ53_08075"
     CDS             2550..3206
                     /gene="udk"
                     /locus_tag="PKZ53_08075"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNJ40431.1"
                     /translation="MHRYHDNNAMIIGISGGTGSGKTTVARKIIQAVGPERVTFVQQD
                     AYYRNLGDMPIELRHRVNFDHPDAFDTELLLNHLEALREGETIEQPIYDYVTHSRKVE
                     AIHTEPRPVIIVDGILIFFSPQMRALMDMKIYVDTDADLRFIRRLERDVHERGRSVDS
                     IVNQYLTTVRPMHLQFIEPLKRYADVIIPKGGDNEVGIDLITEKIKAILARAIPNNQG
                     "
BASE COUNT          831 a          937 c          842 g          785 t
ORIGIN      
        1 cccctgcgaa atccagatat cgtcaaagga tttgagcccg acggcctgtc cccaccactg
       61 ataggcaact tctctgatta gcgtttcccg tggcagttct tttggattat ccagtaatcg
      121 gcgggccagc agcgtgaccc cagccgccgt gtaactttcc agactctcgt tatcaatttc
      181 cgccacctga agcttggtgc caaaggcgta tgggccaaat ttctcattta aaaagcttcc
      241 ggcttcagcc atgatttcgg catagcgttc ggctgagctt tcattgccaa ttttgaaata
      301 gaaatccaca tcaaacccat tgcgctggac ggaacgaacg gcatattcgc ccgccgcaat
      361 cgtgccgggc aacacgggct gagtgactac aaaaatatgt cgggttcgat tgccgcttcc
      421 ggttgggagc ggttttgtgg gcaattgcga ggcttcggtc gttgcttttt ccgtttcggc
      481 tggttgagct ggctttcgtc gcgacccggc tggctggcga tgggttggcg gttttgccga
      541 ttcagtttgt tttcccgtaa ctggtttcac cggtggcggc ggaacaaaag gctgttcggt
      601 gtgttcccca aaggccacca ctttaaattc gcttggtgtt accaggttga tggtgtaggt
      661 ggccacatcc gatgcgtagg cgtgaaaagg aaaccagcgg gcggcataga gcaaataaca
      721 cccgtctttg ccgacatacg ccagacgttt gagcgccaaa accccacctt cagaagataa
      781 aagctgacca gcatattcaa aggtcagggt aaatggttga ccgacaaccg gcgactgacc
      841 aagcgcaact ttcacgctta aatcctgtaa ccgatcctgg acaaactgga gtggttcatt
      901 attggggccc aacaccttcg tcacttcaag cgagccgttg agttcgaggg taactgattg
      961 aacctgccgt gtgaccttca acgtgacaat ggctttggca gccagggttt gggtttcggg
     1021 aatcaactca gcatcaatct tgtagtcaac cacatcaatc acatcttccg cagactgagc
     1081 ctgcgtcggg agcgctgaaa aaatcaaaag cagcatgact cccagagcac atcgcatcat
     1141 aaagcacaca tttactttct tttgcagcca aaagggggag cacatcgaga attctccttg
     1201 ataaaaacac caaaaaacct gttttggaag ttgccggcag tataacttga agcctggagg
     1261 cacgcacgcg ttctgtttct gttgaacttc agatgagcga agttgtcttt gagttaagga
     1321 tgaaaaaacc gggtcagtac cacctgcgta agcgggtggg gttttcaagc caaaagaacc
     1381 cacccgctta cgcaggtggt actgacagat tcttcattca gtaaggtggc gccagccgcc
     1441 gaaagccatg cccgagcaca atcagtaaaa ataccaggtt ccgggctgga agtgccggcc
     1501 agaaatacct gattgggaca gcggatggcc ctggggcggc cagtcggtca gcaatggttt
     1561 ccagaatccg ccttccacgt ggccagtccc agattccagt gcgccacggc aaaggaattc
     1621 ctgactcacc aaccacggtt ccagccagcg caccggtaat cgcagccacc gtgtccgtat
     1681 cgccacccag gtcaagcact gatttgagcg tggcttcaaa atccccgaaa tgccggtacc
     1741 aggcatagag cgcgacagga acggtgtgaa acatatagcc ggaaacacct cgcgacaatc
     1801 caagtctgtc agcaaatgtt tcaactgaaa gctcctgatc aattgcggtt gacactttct
     1861 ggagcaattc ctgccagtcg gcatccgttc ctacctgatg cagtagtgcc aggaaatctt
     1921 cttttgaggg tcgggagacc aaattttcac gcatcaccca ggcggcgata tcggccacgg
     1981 ctcgggcacc aatcaccgct tttgggtccg tatgggtgag tcgggttgaa gcagtcacaa
     2041 aggtggttct cagttctgga ttttcagcaa aaaatgcccc aatgggggct gaacgcatcg
     2101 ccggcccgtt tccagccgag aaaactccgc tccgactggg agggaaccca atccacagtt
     2161 tcaaggttgc ccgcaacgtc gccaccccaa tcccggctgg cagtgacgcc acccatccac
     2221 gcaaacacca ccccaatcgg ctggtgaaac gctcgacaga gtctggatgc gctagtaaac
     2281 tctgagccac gaaaaccgtg tggtcggtgt catcactcac catcccccaa ccgaaacaaa
     2341 atcggtgacg ccaggttcca ccataccatt ttcgaattcg ttgtcgggac agcccttcgg
     2401 ctggtaatcc aagcgcatct ccgacagccg tcccaagcat taaccctctc agccgttgtg
     2461 ttcgatccat ctttttgtgt atttggagga agtcgtctgt tgtttgtcca gtgcatctgg
     2521 ctcaggcgaa gatcaaaatc cactttgtta tgcaccgtta ccacgataat aacgctatga
     2581 tcatcggtat cagcggcggc accggttcgg gaaaaaccac ggtcgccaga aaaatcatcc
     2641 aggctgtcgg tccggaacga gtgacctttg tccagcagga tgcctattac cgtaacctgg
     2701 gagacatgcc gattgaactt cgccaccgag tgaattttga tcatcccgat gcttttgata
     2761 ccgaattact tttaaaccat cttgaagcct tgcgtgaagg cgaaaccatt gaacaaccaa
     2821 tttatgacta tgtcacccac tcacgcaaag ttgaagccat ccatactgaa ccacgtcccg
     2881 tcattattgt tgacggcatc ctcattttct tcagcccaca aatgcgggcg ctgatggaca
     2941 tgaaaattta tgttgatacc gatgctgatt tgcggtttat ccgccgactc gaacgggatg
     3001 tccacgaacg aggccggtcg gtggattcga ttgtcaatca gtacctcacg acggttcgcc
     3061 ctatgcattt gcaatttatc gaaccgctca aacgctatgc ggatgtgatc atccccaaag
     3121 gtggcgacaa cgaagtcggc attgacctga tcaccgaaaa aatcaaagcc attctggcgc
     3181 gagccattcc gaacaaccag ggctgaagac tcgcaagctc ggggctgaaa acgttgggct
     3241 cagggctgaa aacttcgggc tcaaaaaaac tgacgactgt ttttagatga atttcaaccc
     3301 caagcccgtt ttcttcagcc ccaagccctg agccctgggt ttttcagccc caagcccatc
     3361 ttcttcagcc cgatgtcttc agccctgggt ttctc
//
LOCUS       DAOQDO010000424         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21127_length_3394_cov_3.012878, whole genome shotgun sequence.
ACCESSION   DAOQDO010000424 DAOQDO010000000
VERSION     DAOQDO010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21127_length_3394_cov_3.012878"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKZ53_08080"
     CDS             <1..1065
                     /locus_tag="PKZ53_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNJ40432.1"
                     /translation="EVGGNPGRGSYRVAVEAERGLIDVRGKLARLIKAATPDRVIFTC
                     NTTDALNMAIKGALREGDHVITSELEHNSVLRPLARLKQTRQIQVTTLPFDVRGQVDP
                     DAFRRAITPRTRMLILTHASNVIGTIQPIEEVGKIARDHNLLFLVDAAQTLGKVDINV
                     QTANIDLLAGPGHKGLMGPPGTGFLYVGERAVLEPWREGGTGGNSLLELQPPEYPFFL
                     EAGTPNTVGLAALGVALEYLAERGIDRIREHELQLLELLADELCQLPDVSIFGPPDLD
                     HAVGVISFTMEVFEPQEVGIVLDESFDIAVRSGLHCAPLTHKRLGTSPTGTIRVSPGA
                     FTTVADIEQFISALKTLALS"
     gene            complement(1125..1442)
                     /locus_tag="PKZ53_08085"
     CDS             complement(1125..1442)
                     /locus_tag="PKZ53_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40433.1"
                     /translation="MRLAKTDRCQIDEQEILAAQLDLQTFSTLFPVELEEVHPLSVPA
                     SPCLGGLIQLDNGQYLFLYYCFPLTCASVLKPVGQPTREVLAHLFQEVPDLRDFVTFI
                     EAH"
     gene            complement(1767..2357)
                     /locus_tag="PKZ53_08090"
     CDS             complement(1767..2357)
                     /locus_tag="PKZ53_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40434.1"
                     /translation="MTAQIPDFFRLDNDEFELTAFQGQEIFSPSTLGLSPVAATTACW
                     RGFGCQFGIIQNSLVLEELWVTNGELIEGVFHPQPGPSIDGVTPDSGEKLGIFFNCRY
                     CGLHLPVKFTGGILLGKEYLVDGYEWAAFKRPWQFQKVIELVFEAGKLIQMTNLSQIM
                     CLVREQVEEKLLSPFIEEREQARSWIQKTYNLDYHF"
     gene            complement(2354..2902)
                     /locus_tag="PKZ53_08095"
     CDS             complement(2354..2902)
                     /locus_tag="PKZ53_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40435.1"
                     /translation="MEHSTPTSGQYCEIESVLLGSGRLVLLGWREPISVYYSIKPSIE
                     KTYILKYQDEDGEIEISSLVDLKLVVEVKRLNLVTEISGRETTFATIFSYLFELGQVI
                     RSVYGRFGYLEDSYKIPLQPNRPRLEDAVLRFLAIPFSLEDLHPPMWDIFIDLNGYEL
                     KKVLIGQLFPKSEAVQPDGFTQ"
BASE COUNT          920 a          823 c          793 g          858 t
ORIGIN      
        1 gaggtcggcg ggaaccctgg gcgtggaagt taccgcgtgg cggttgaagc cgaacgagga
       61 ttgatagatg tgcgggggaa actggcacga ttgatcaagg cggcaacgcc cgaccgggtt
      121 atttttacgt gtaacaccac tgatgcgctc aatatggcga tcaaaggagc actccgcgaa
      181 ggtgaccacg tcatcacgtc tgagctggag cataattctg tgttgcggcc tctggcgcga
      241 ctcaagcaaa cccgccagat tcaggtcacg acgctcccgt ttgatgttcg tgggcaggtt
      301 gatccagatg cctttcggcg agccattaca ccccgaaccc ggatgctgat cctgacgcat
      361 gcttcaaatg tgatcggcac cattcagccc atcgaagaag tcggaaaaat tgcccgcgac
      421 cacaatctgt tatttctggt tgatgcggcg caaaccctgg gcaaggttga tatcaacgtt
      481 cagacagcca atattgacct gctcgccggg ccaggccata aaggattgat gggcccgcct
      541 ggaaccgggt ttctctacgt tggagagcga gctgttttgg agccctggcg cgaaggcggc
      601 accggtggta attcattgct cgaactgcaa ccacccgaat atccattctt tcttgaagcc
      661 gggaccccaa acacggttgg attggcggcg ctgggtgtag cactcgaata ccttgccgaa
      721 cgcggcattg atcgcattcg cgagcacgaa cttcagttgc ttgaactcct ggccgatgag
      781 ttatgccagc ttccggacgt ctcaattttt ggaccaccag acctcgatca cgccgtgggt
      841 gtgatttcat tcacgatgga agtctttgaa ccacaagaag ttggaattgt acttgatgaa
      901 tcgtttgata ttgccgttcg gtcagggctg cactgtgccc cattgactca taaacggctt
      961 ggtacatcgc caacgggcac tatccgggtc agtcccggag catttacaac ggtggccgat
     1021 atcgaacagt ttatttcagc cctgaaaacc ctggccttga gctgaggaat gaagaatgaa
     1081 gaattgagaa tgaagaaaac acctgcgatg agttacctct ttggtcagtg ggcttctata
     1141 aacgtgacaa agtcgcgtaa atcaggaact tcctggaaaa gatgagctaa aacttccctg
     1201 gtgggctgac caactggttt gagcactgag gcacaggtca ggggaaagca ataatacaga
     1261 aacagatatt gcccgttgtc gagctggatc aacccgccaa gacagggtga ggcgggaaca
     1321 gacaatggat gcacttcttc aagctcaacc ggaaacaggg ttgaaaaagt ttgaaggtcg
     1381 agttgagcag ccaggatttc ctgttcatca atttgacatc tatcagtttt tgccaatcgc
     1441 atcaaacctc ctggaaccga catcttctat ttattccgaa ctggtttctg gtcaaatgcg
     1501 ccatatccat gtgcgtctaa ctccctcagt ccatttaaga aaacctcttc ttccaaaccc
     1561 aaatcagttt tgaaaccatt cccccggtac aaactttgta aattgagcgt gaacttgatc
     1621 aaatcttcgg gttggtgaac gtctatgcag agaaattccc aaatttgtct ggcggtcaaa
     1681 gtgatggttc gatcagtttc cattcgtgtt ttttgtcttt gaaactcaag aaaccaggct
     1741 ttctggtcgg tactcatcgg catttcctag aaatggtagt caagattata ggtcttttga
     1801 atccagcttc ttgcctgttc tctttcctca atgaacgggc tcaagagttt ttcctcaacc
     1861 tgttctctta caagacacat tatttgtgaa aggttggtca tctggataag tttcccggct
     1921 tcaaagacaa gttcaatgac cttttgaaac tgccagggac gtttgaatgc agcccattca
     1981 tatccatcaa ccaaatactc ttttccgagc aatataccac cagtaaattt gaccggtaag
     2041 tggagcccac aatatcggca attgaaaaaa atgcccagtt tttcacctga atccggtgta
     2101 actccatcaa tagacggccc aggttgtggg tgaaaaacac cctcgatcaa ttctccattt
     2161 gtgacccaaa gttcttccaa aaccagagag ttctgaatga ttccaaattg acacccaaac
     2221 ccacgccagc aggcagtcgt ggctgccaca ggcgataatc cgagcgtgga aggcgagaaa
     2281 atttcttgcc cctggaaagc agtgagttca aattcatcat tgtcgagtcg aaaaaaatct
     2341 ggaatttggg cagtcattgg gtgaaaccat caggttgtac agcttcagat ttggggaaaa
     2401 gctggccaat caacactttt ttcagttcat acccgttgag atcaatgaag atatcccaca
     2461 ttggagggtg aagatcctct aaactaaagg ggatggccag aaatcgaagt acggcgtctt
     2521 caagtcgtgg cctgttgggt tgaaggggaa tcttatagga atcttccagg tagccaaatc
     2581 tcccatagac tgaacggatg acctgcccta actcaaataa ataagaaaaa atcgtggcaa
     2641 acgtggtttc tcgaccactg atttcagtaa ccaggttgag tctcttaacc tcaacaacca
     2701 gtttgagatc aaccaaagag ctgatttcga tttccccatc ctcatcctgg tattttagaa
     2761 tataagtctt ttctattgaa ggttttatgg aatagtacac agaaattggc tcacgccatc
     2821 caagaagcac cagtcttcca ctgccaagca aaacagattc aatttcacaa tattggccac
     2881 tggttggggt ggaatgttcc attttcacgc atcccttccc agaactttca acttttcagt
     2941 ttctaccata gataggctga cctgttcagc attcagatca gtcttttgga gaaaaccgat
     3001 caggagttgg tgataaaccc cgctttttga tacgtcgcct ggtcagatag gtatcaaggc
     3061 aaatgagttc aatcagcgtt ggaacaaaac agatcatcag cgctatcaca tactgtcgag
     3121 gcgttatctg attcatatcc acaagtttct ggtaaatgaa cagtagcaac acagcattga
     3181 ataccagaca aaggccaaaa atgaaggtgc ccagatttat gatcagacac ccgcgaagaa
     3241 ttgacataag tgagtcccca cctgcaacgc ccctggctca tcgaggccat tgttgccaaa
     3301 ctcaatggca gtcaactctg gtgatctcct tccaaaaccg tgaacctctc ggccataaat
     3361 ggccgagctt ctgggtggga tcatccaact caca
//
LOCUS       DAOQDO010000425         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21154_length_3392_cov_2.764459, whole genome shotgun sequence.
ACCESSION   DAOQDO010000425 DAOQDO010000000
VERSION     DAOQDO010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21154_length_3392_cov_2.764459"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKZ53_08100"
     CDS             <1..231
                     /locus_tag="PKZ53_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNJ40436.1"
                     /translation="AKRQLLVPQSEDVSRHIPQDVKTAVWQRDKGKCVQCASASYLEF
                     DHIIPFSLGGASTLNNIQLLCRKCNLAKGNRL"
     gene            664..>3392
                     /locus_tag="PKZ53_08105"
     CDS             664..>3392
                     /locus_tag="PKZ53_08105"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNJ40437.1"
                     /translation="MNTRGHKLLFFLIFLLILVTLFFSFPIPIVITSTQEQEKVAPVT
                     ASPFSETQERCLLSERDRFEPLAPVLAQPWVSQPSLPEPLIGEVELKTEAVTPDSGLI
                     KDNLLVVNRFTPSSYPAMLQRVRIRFDQFQGFPSPVGQTVRLVAFLDPNGLGKPPGSP
                     AFITQELITIPAVGEFSEFGLNPVTILSGDLYVGYQVSDPAQGAGFSTVMDGPQSDRA
                     FYSTSNGEGFAGPFQFSSGQHANVCIRAVVSGMQSTDEELKTDDGTLDGRGVIDNGLL
                     IVNRLTPSSYPATLKTIRIYFASFQGYASPVGQSIRLVAFTDPSGSGQPPSNPTLAVN
                     QSVTIPRIGSFVDFAVSGPTISAGDFYVGYQAPNPHNGVGFPVDTNGVAQRRGFFSID
                     NGATYLGPIQFTDGTQMNVMIRAVVSKQTGPSGDFSLAANPTSQTIAPGSSGVFSVMT
                     TPINGFAQNISLTTSVSPADSTVQARLATSTVSPGGGTTLTFSTSTGTPQRSYTVTIT
                     GTSSGLTHSTSVTAVVITGGTSTMTLEATDNIAAEIGGNSGTVTVTRTGTSTTALTIN
                     YQVSGTASAGRDYMALSGGVLMQAGAAKATISIIPLDDSETESTETVIITLTPGSGYQ
                     IGTPSTATVSILDNESLPTLKFASSTLTVSELSMANCVITATGNIDWAMAVKYRFAGS
                     AVMGNDYLELPETMPVAKGQTQISIPLTLMPDSENEGDETILVEIVPDSTYQVGSPAT
                     MTITVKDCTEPNGAMVDADQGAVVSGEDGAMAIFAPAPGDESFTATWAKTKKTPKKLK
                     VKNDFAPISGVYKLTLAGKILRDKAVVVGFPIAPALLPTNPAERVFRLEYQIDGTKDW
                     MPEGMLVSFDPVTSSAYFATTIPSSFSAQAATLSEGFEPLQSTTLVRTYRIQLS"
BASE COUNT          792 a          913 c          887 g          800 t
ORIGIN      
        1 gccaaacgcc agcttctggt cccgcaaagc gaagatgtca gccgacatat tccacaagac
       61 gtcaaaacgg ctgtctggca acgagataag ggaaagtgtg ttcaatgtgc ctcagcctca
      121 tacctcgaat ttgaccacat cattccattt agtttgggag gcgccagcac gctcaataat
      181 attcagcttt tgtgtcgaaa atgtaacctg gccaagggaa atcggcttta agttggacag
      241 caggaaatga gtcaggttga gttcaagatt gggtttcggc tcaagttttg aggcttcttc
      301 gaattaaaga aactgagcct ggtgaatcca cagatgaatg tgcaaaatca cagtccaaaa
      361 atgatgacat tcttttgaac cacagaggag cacagaggac acagaggcaa accaatcaag
      421 cgggacttcc tctgtgctcc ttcggcgtcc tctgtggtga aattgtcttt cctgatgaca
      481 atagaggatt tttaccgggt agtagggaaa atataccagt tttgttcgct ggtcggctga
      541 ctgatctgcc gcaggtctcg acctggcgca cctcacccaa aaatccaact ggcataggga
      601 ttgcccttta tagtctgctg tctgctttgt gtgtgttcga tggcagcaga agggaggcat
      661 cccatgaaca cccgtggaca taaactcctg tttttcctga tctttctgtt aatccttgtt
      721 accctcttct tttcgtttcc aattccaatt gtaattactt caacccagga acaggaaaag
      781 gtggcgccgg taacggcgtc accgttctct gaaacccagg agcggtgcct gctctctgaa
      841 cgagaccggt ttgaaccgct ggctcctgtt ttagctcaac cctgggtttc ccaaccctcg
      901 ctgccggaac ctctcatcgg ggaggtggag ctcaaaaccg aggccgtcac cccagattca
      961 ggtctgatca aagataacct gctggtggtc aatcggttca cgccctccag ctatccggca
     1021 atgctccaac gggtgcgaat tcggtttgat caatttcaag gatttccatc gccggttgga
     1081 cagacagttc gactggtggc ctttttggat cccaatggcc tgggcaaacc gccggggtca
     1141 ccagcattca tcactcaaga attgattaca attcctgccg ttggcgaatt ctccgaattt
     1201 ggactcaacc cggtgaccat cctttcaggt gacctctatg taggttatca ggtgtcggat
     1261 cctgcccagg gcgctggatt ttcaaccgtt atggatggtc ctcagtctga tcgggcgttt
     1321 tattcgactt caaacggcga aggattcgcc gggccgttcc aattttccag cggccagcac
     1381 gccaatgtgt gcattcgggc ggttgtttcc gggatgcaat ccactgatga agaactcaaa
     1441 accgatgacg gaacactcga cggacgcggc gtgattgata acggtttgtt gatcgtcaac
     1501 cgattgacgc cgagtagcta tccggccacg ctcaagacaa ttcggattta ctttgcgtcg
     1561 tttcaaggat acgccagccc ggttggccaa agcatccggc tggtggcctt cacggatcca
     1621 tccgggagtg gtcagccgcc atcaaatccg acgcttgccg tcaatcagtc ggtgactatt
     1681 ccacgcattg ggtcatttgt tgactttgcc gtgagtgggc cgacgatttc cgcaggtgat
     1741 ttttatgtcg gctatcaggc gcccaatccg cacaatgggg ttggcttccc ggttgatacc
     1801 aatggagttg cccagcggcg tgggttcttt tcaattgaca acggagcaac ctacctgggg
     1861 ccaatccagt ttactgacgg aacacaaatg aatgtcatga ttcgtgctgt cgtcagtaaa
     1921 caaaccggtc cgagcggtga cttttcactt gcggccaatc cgacttcgca aaccattgct
     1981 cctggcagca gcggtgtctt ttcggtcatg acgaccccaa tcaatgggtt tgcccaaaat
     2041 atttccttga cgacttcggt tagtcctgcg gacagcacgg ttcaggcccg actggcgacc
     2101 agcaccgtct cgcccggtgg cggcacgaca ttgacgttta gcaccagcac cggaacaccg
     2161 caacgcagct acacggtaac gattaccggg acgtcgagcg gcttgaccca cagcaccagt
     2221 gtcacggcag tggtgattac cggggggaca tccacgatga ctctcgaagc caccgacaat
     2281 attgcggctg agattggagg caattccggc acggtaactg ttacccgaac cggcaccagc
     2341 acaacagcct tgacgatcaa ctatcaggtg agcgggacgg cttcggcggg gcgtgactat
     2401 atggccttgt ctggcggcgt gttgatgcag gccggagccg ccaaagccac aatttcgatc
     2461 attccgcttg atgacagcga aaccgaatcc accgaaaccg tcatcatcac cctcacccca
     2521 gggagcggct atcagattgg aaccccgtca accgccacgg tttcaatttt ggacaacgaa
     2581 tcgcttccaa ccttgaaatt tgcttcgtcc acgctgacgg tttcagagct ttcgatggca
     2641 aattgtgtga ttaccgccac cgggaatatt gactgggcga tggcggtcaa atatcggttt
     2701 gccggttcgg cggtgatggg caacgactat cttgaactcc ctgaaacgat gccggttgca
     2761 aaaggtcaga cccagatcag tattccgctg acactgatgc ctgattcaga aaatgaaggc
     2821 gatgaaacga tcctggttga aatagtgcca gatagcacgt atcaggtcgg ttcaccggca
     2881 acgatgacca ttaccgttaa agattgcacc gaacccaatg gcgcgatggt ggatgccgat
     2941 cagggcgccg ttgtgtcagg cgaagatggc gcgatggcga tttttgcgcc ggcgccaggc
     3001 gatgaatcct tcaccgcgac ctgggccaaa acgaagaaga caccgaagaa actcaaagtc
     3061 aaaaatgact ttgcgccgat ttccggggtt tacaaactga cgctggccgg aaaaatcctg
     3121 cgtgacaaag cagtggtggt tggatttcca attgctccgg cgctgctgcc gaccaatccg
     3181 gcggagcgag tgtttcggct cgaataccag attgacggca ccaaagactg gatgcccgaa
     3241 ggcatgctcg tcagttttga cccggtgacc agttcggcgt attttgcgac gacgattcca
     3301 tccagtttct cagctcaggc tgcaaccctc tccgaagggt ttgaaccgct ccagtcaacc
     3361 acgctcgtgc gaacctatcg cattcaactt tc
//
LOCUS       DAOQDO010000426         3389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21179_length_3389_cov_4.058788, whole genome shotgun sequence.
ACCESSION   DAOQDO010000426 DAOQDO010000000
VERSION     DAOQDO010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21179_length_3389_cov_4.058788"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="PKZ53_08110"
     CDS             <1..1238
                     /locus_tag="PKZ53_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40438.1"
                     /translation="KIAPRIGAAWDVLGDGKIKVSGSFSIFYDTMKYELPRGSFGGEK
                     FLRTYRKLEQADYRGINLTNQPGDVILGPIDLRFPSNVTLPGSRPTIDPDLKPFKMRE
                     FSFAVDYNFSKDWLFSARFTRKDVLRAIEDVGGLDSQGNEVYTIGNPGFGATVDFFDP
                     PTPKAVREYTGLELRLDKRFSKNWYANFSYIYSKLYGNYSGLASSDEITNGVGRTSPN
                     VNRNFDLPALNFDANGDILLGRLPTDRPHTFKTFAAYRFDYKGFGKNMSTEIGGSQFI
                     YQGTPITTTVNSTVEGSSGLPLFPFGRGDAGRTEVFTQTDLVVSHFINVSERVRFKFS
                     FNVFNLFDERNQTDLFNGILGPGQNVNFATLAEYLSSTPDGVKQRISSQNLVLDPRYK
                     QAVGFQNPREARISFGIQF"
     gene            1443..2504
                     /locus_tag="PKZ53_08115"
     CDS             1443..2504
                     /locus_tag="PKZ53_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNJ40439.1"
                     /translation="MKSRLIGIVLMVGQAVIPSTQLSVPATVMIQSRNTVQGRVAGTD
                     NRPVQNVRVFLLNDGYSQVGMQYSDSAGRYRFGNVPAGNYYLQVEPAGTEFEAQTVRI
                     EVNPVNTLGTGGEVFTYDLNLRPRKNPVSSGEALPAKNDSSVFYQDVPEAARKEYKKA
                     AKSFEKKEVEPGVAALKKALEIFPEYYDAMELLGSTYVDAGDYPAALPLLVKAVEIND
                     AGWHGFYSLGVACVELKHQKEGLLALRKAAELNPTSPNVNMRLGMVLAQSEATFPDAI
                     DAFQKVIHVAGKQIPQAYLYLATIYSKRNQNREAADALEAYLQSIPADAAQKEQREKM
                     KKVIEQLRQKAAVDDNGSK"
     gene            complement(2763..>3389)
                     /locus_tag="PKZ53_08120"
     CDS             complement(2763..>3389)
                     /locus_tag="PKZ53_08120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099640.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNJ40440.1"
                     /translation="SEVSALLGRMPSAVGYQPTLATEMGELQERITSTKNGSITSVQA
                     VYVPADDLTDPAPATTFSHLDAKTVLSRQIAELGIYPAVDPLDSSSRILDPRIVGQDH
                     YAVAEGVKRVLQRYKELQDIIAILGIDELSEDDKLTVARARKLQKFLSQPFHVAEQFT
                     GIKGAYVKLADTIRSFKEILDGKHDDLPEQAFYLVGTIDEVVERAKSM"
BASE COUNT          849 a          876 c          876 g          788 t
ORIGIN      
        1 acaaaatcgc gccacgcatt ggtgcggcgt gggacgtgct gggtgatgga aaaatcaaag
       61 tttccggtag cttcagcatt ttctatgaca cgatgaagta tgaattgcca cgcggttcat
      121 ttggcggtga aaagttcctg cgtacctatc gcaaactgga acaggctgat tatcggggca
      181 ttaacctgac caaccagcct ggtgatgtca tccttggacc gattgacctg cgcttcccgt
      241 caaacgtgac gttgccaggt tcacgcccaa cgattgatcc agacctcaaa ccgttcaaaa
      301 tgcgtgagtt ctcatttgcc gtggactaca acttctcgaa agactggctc tttagcgccc
      361 gctttacccg caaggatgtg ctgcgtgcga ttgaagacgt cggtggtctc gactcccagg
      421 gcaatgaagt ctacacgatt ggcaaccccg gtttcggtgc caccgtggat ttctttgatc
      481 ctccaacccc gaaagccgtg cgtgaataca ccggtcttga actgcgcctt gacaagcgtt
      541 tctccaagaa ctggtatgcc aatttctcat acatctacag caagctgtat ggaaactact
      601 ctggtctggc cagctcagac gaaattacca atggcgtagg ccgtaccagc ccgaacgtca
      661 accggaactt tgacctgcca gccttgaact ttgatgccaa cggtgacatt ttgttgggtc
      721 ggttgccaac ggaccgtccg cacaccttta agacatttgc tgcctatcgc tttgattaca
      781 aaggctttgg gaagaacatg tccactgaaa tcggtggttc acagttcatc tatcagggca
      841 caccgatcac caccaccgtc aattccaccg tcgaaggttc cagcggtctg ccgctgttcc
      901 catttggtcg cggcgatgcc ggacggactg aagttttcac ccagaccgat ctggtggtgt
      961 cacactttat caacgtcagc gaacgcgtcc gctttaagtt ctcgttcaac gtgttcaacc
     1021 tgtttgacga acgcaaccag actgacctgt tcaacgggat tttgggccct ggccagaacg
     1081 tcaactttgc gacgttggcc gaatacctgt catccacgcc agatggcgtg aaacagcgca
     1141 tcagctcaca gaatctggtg cttgatccac gctacaaaca ggccgtgggc ttccagaatc
     1201 cacgtgaagc ccgtatttcc ttcgggattc agttctaaaa aagaagtacc ttcgggtact
     1261 ccgtgcctgg ctctttattc ctggaaaata ttgagaatca attatttatc aggaatcagg
     1321 ccgaaagaac caggtttgtg cgcacgaaaa tgatcctgtg atcaagtgac cgcgcactgt
     1381 atgataatgc ccgtctcgac tcgtgcgagg cgggcatttt tttgcaaagg agtgagatgt
     1441 gtatgaaatc caggctgatt ggaatcgtac tgatggtggg gcaggcagta attccctcga
     1501 cacaacttag tgtaccagca acggttatga ttcagagccg aaacacagtt cagggacgtg
     1561 ttgcgggaac tgacaatcgc ccggtgcaaa atgtacgggt gtttctgctc aatgatggtt
     1621 actcacaagt cggtatgcag tattccgata gcgccgggcg atatcggttt ggcaatgtac
     1681 cggcaggcaa ttattacttg caggttgaac cggcaggaac agagtttgaa gcgcaaactg
     1741 tgcgcatcga ggtcaatcca gtgaatacac tcggcactgg aggcgaagtc tttacctatg
     1801 atctcaatct gcgtcccaga aaaaatccag tcagttccgg cgaagccttg ccagccaaaa
     1861 atgactccag cgtgttttat caggatgtgc cggaggctgc ccgcaaagaa tataaaaaag
     1921 ccgccaaatc ctttgaaaag aaagaagttg agccaggggt tgctgccctg aagaaagcac
     1981 tggaaatttt tcctgaatat tatgatgcga tggaattatt aggcagtacc tatgttgatg
     2041 ctggggatta tccggcggcc ctccctttgc tggtcaaagc ggttgaaatc aatgatgctg
     2101 gctggcacgg gttttattcg ctgggtgtgg cctgtgttga actcaaacac cagaaagagg
     2161 gtctgctggc tttgcgtaaa gctgccgaac tcaacccaac ctccccgaat gtgaatatgc
     2221 gactggggat ggtgctggca caatctgaag ccacattccc cgatgcgata gacgcttttc
     2281 aaaaagtaat ccacgtggcc gggaagcaaa tcccgcaagc ctatctgtat ctggccacca
     2341 tttacagcaa acgcaaccag aaccgcgaag ccgccgatgc cctcgaagca tacttacaat
     2401 caatccccgc tgatgctgct cagaaagaac agcgcgaaaa aatgaagaaa gtcatcgaac
     2461 aactccgcca gaaagccgcc gttgatgaca acgggtcaaa gtgacaaagt gacaaaatga
     2521 aaaaaagcgg gatcgcacat atggtgtgcg atcccgcttt gtagtcagta gttgatgagt
     2581 cagtcgtcag tagttgatga gtcagtcgtc agttgtcagt agaccaaccc cagatgtttc
     2641 agtcagcaga gatcgtaaat gactcaaaaa gaaccaaatc cactgaacac aaattccaaa
     2701 atcccaacac tcgactaaga actaagagcc aagagccagg gaccaagaac tactgactgg
     2761 tcttacatgc tctttgcgcg ttcgaccact tcgtcaatcg tgccgaccag gtagaatgcc
     2821 tgttccggca gatcgtcgtg tttgccgtcg aggatttcct tgaagctgcg gatcgtgtcg
     2881 gccagtttca cataggcacc cttgataccg gtgaactgtt cggccacgtg gaacggctgt
     2941 gacaggaatt tctggagctt acgggcacgg gccaccgtca gtttgtcgtc ttctgacagt
     3001 tcatcaatcc cgagaatggc gataatgtcc tgcagttcct tatagcgctg caacacgcgc
     3061 ttgacgcctt cagccaccgc atagtggtcc tggcccacaa tgcgtgggtc gaggatgcgc
     3121 gatgatgagt cgagcggatc caccgccgga tagattccga gttccgcaat ctgacgcgaa
     3181 agcacggttt tggcgtcaag gtgcgagaag gtcgtggctg gtgccggatc ggtcaagtcg
     3241 tcagccggca cgtacacggc ctgcactgac gtaatcgaac cgttcttggt cgaggtgatg
     3301 cgttcctgca attcacccat ttccgtggcc agcgtcggct ggtagcccac ggcggaaggc
     3361 atacggccaa ggagcgccga tacttctga
//
LOCUS       DAOQDO010000427         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21190_length_3388_cov_2.602760, whole genome shotgun sequence.
ACCESSION   DAOQDO010000427 DAOQDO010000000
VERSION     DAOQDO010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21190_length_3388_cov_2.602760"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKZ53_08125"
     CDS             complement(<1..202)
                     /locus_tag="PKZ53_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40441.1"
                     /translation="MPRPRSRKTDTRDDAAMAVESPSAPQLVVAEPEVIVPVETLSAV
                     NVPATKRTRAKKQKLEPVPDVPA"
     gene            complement(289..1533)
                     /locus_tag="PKZ53_08130"
     CDS             complement(289..1533)
                     /locus_tag="PKZ53_08130"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ40442.1"
                     /translation="MSSNPELPPPSSRVCHNCHQLTPNAVPRCLECGAVNPEFLEEQE
                     IHRFIQTFFFRKALFSYIFLAINLCIFVLMMFAGSTTDPAVLMAFGAKQNDLIRNGEY
                     WRLVTPMFLHIGLIHLGFNCYALWTLGPRIESLYGSARFVVIYIFTGIFSIYASYVAS
                     PNPSAGASGALFGLFGTLYVFALRYPNVIPPLLRKNMLSSLNSLLLINLVLTFILPVS
                     ASAHIGGLVAGGLAGWLIPYSPPREQKTPAGWIIGQVVALALVIASFGVLVWRYEGPR
                     LSLHEFRAGSSLWFSEDSGGPVNRFVEAINTAERSFKEIQGRFETEELTPALREQFKT
                     KNREAQEKLASAPRLEPGSEKLKQELMAVLKAQEQWLATGDHAQADELMRKFNQYDQN
                     CRQWVETTGKRFSLVVDEEANK"
     gene            complement(1670..2416)
                     /locus_tag="PKZ53_08135"
     CDS             complement(1670..2416)
                     /locus_tag="PKZ53_08135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ40443.1"
                     /translation="MIPLRDNVPSQSFPIVTVLLIAANVLAFIYELLLGPRLTAFFYS
                     YAVVPRYYFSDFVILPGGGMRPVETAQLVIPLFTSMFLHGGWMHLLGNMFYLWIFGDN
                     VEDRMGKFKFLIFYLLCGLLASTAHIVTNRTSQVPSLGASGAIAGVLGAYLVLYPWAR
                     ILVLNIFIILTEFEVPALFFLGLWFVQQFIAGLASLGPASAQTGGTAWWAHIGGFAAG
                     VILVKLFINRPQPLILQPTWPDPDDDDRFI"
     gene            2535..3242
                     /locus_tag="PKZ53_08140"
     CDS             2535..3242
                     /locus_tag="PKZ53_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ40444.1"
                     /translation="MHIVVIIPALNEALSIGKVLDDIPRPPVTDVIVVDNGSTDGTPA
                     IAASHGARVVTEPQRGYGAACLRGLSEIGPEAEIIVFLDGDYSDYPEEISALIEPICR
                     GKADLVIGSRELGDREAGALMPQQRFGNALATGLIRLLWGHRYTDLGPFRAIRRNSLD
                     QLHMQDQNYGWTIEMQIKAIRYGLRIHEIPVRYRCRIGVSKISGTVMGSFNAGRKILS
                     CIARFWWEDRFGLKARG"
BASE COUNT          901 a          873 c          891 g          723 t
ORIGIN      
        1 gtgccggtac gtcgggaact ggctcaagct tctgtttttt cgctcgtgtc cgtttggtcg
       61 ccggtacgtt gactgccgaa agtgtttcaa ccggtacgat gacctccggt tcggcaacga
      121 caagttgggg ggcactcggg gattcaaccg ccatagcagc gtcatccctg gtatcagttt
      181 tacgagaacg tggtcgcggc ataaaaacct cactcactca aggtcactgg cttggaaaat
      241 atggaagaaa agagaaaccg gcgtggggca actgacgcgt gaattaagct atttgttggc
      301 ctcttcgtca actaccaaac tgaatcgttt tccggtggtc tcgacccatt ggcggcaatt
      361 ttggtcatac tggttaaatt ttctcatcag ttcatcggcc tgagcatgat caccagttgc
      421 aagccattgc tcctgtgcct tcaacacggc cattaattcc tgcttgagct tttctgaccc
      481 cggctcaagg cgtggtgcac tggccagttt ttcctgagcc tcacgatttt tggttttgaa
      541 ctgttcccga agcgcgggtg ttagttcttc ggtctcaaac cggccttgaa tctctttaaa
      601 cgagcgctcc gccgtgttga tagcttcgac aaaccgattg accggcccgc cagaatcctc
      661 gctaaaccag agacttgacc cggcccgaaa ctcgtgcagt gacagtcggg gaccttcgta
      721 gcgccagacc agcacaccaa atgaagcaat caccagggcc agggcgacca cctgcccaat
      781 gatccaaccc gcgggcgttt tttgctctcg cggtggcgaa tatggaatca gccagccagc
      841 caatccgcca gcaaccagcc caccgatatg ggccgaagcc gacacaggca agataaacgt
      901 gagcaccaga ttgatcaaca acaaggaatt gagcgaactg agcatgttct tgcgcagcaa
      961 gggcggaatc acgttgggat atctcagagc aaagacatag agcgtcccaa ataacccgaa
     1021 caacgctccc gaagcaccag cgcttggatt tggactcgcc acataactgg cgtaaatgct
     1081 gaagatcccg gtgaaaatat aaattacaac aaaccgggca gagccataga gtgattcaat
     1141 tcgcggcccc aacgtccaga gtgcgtaaca gttaaacccc aggtgaatga gcccgatgtg
     1201 cagaaacatc ggtgtgacca gccgccagta ttctccgttg cggatcaagt cattttgttt
     1261 tgccccaaag gccatcaaaa ctgccgggtc ggtggtgctc ccggcaaaca tcatcaggac
     1321 aaagatacac agattgatcg caagaaaaat gtaggaaaac agcgccttcc gaaaaaagaa
     1381 cgtctgaatg aatcggtgaa tttcctgctc ttcgaggaat tcaggattca ccgcaccaca
     1441 ttcgaggcac cgtggcaccg cattcggggt caactggtgg cagttatggc aaacgcggga
     1501 tgatggaggt ggaagttcag gattgcttga cactggaatt ttagggtttg gggttcagaa
     1561 ttagagttca gggttcaggg ttcagggttg agcaaacgac cagaaagact caaaggactt
     1621 aaaggacaca aatttgaaac cccgaacccc gaaccctgaa ccccgaaccc taaataaacc
     1681 ggtcatcatc atccgggtcg ggccaggttg gctggagaat caatggttgt gggcgattga
     1741 taaagagttt gaccagaatg accccagcgg caaatcctcc gatatgcgcc caccaggctg
     1801 ttccaccagt ctgggcagat gccgggccta aactggccag cccggcaata aattgctgga
     1861 caaaccacaa tccaagaaag aaaagggccg gaacctcaaa ctcggtcagg ataatgaaaa
     1921 tattgaggac caggattcgt gcccagggat agagcaccag ataggcgccg agcactccgg
     1981 caatggcacc gctcgcgcca agtgatggga cttgtgaggt tcggttggtc acaatatgag
     2041 cggttgacgc cagcaagccg cacagcaggt aaaagatcaa aaacttgaat tttcccattc
     2101 gatcttcgac attgtcaccg aaaatccaga ggtaaaacat gtttcccagc aggtgcatcc
     2161 agccgccgtg caggaacatg gacgtaaaca aagggattac aagctgtgcc gtttcaaccg
     2221 gtcgcatccc accacctggt aaaatgacaa aatcgctgaa atagtaccgt gggacgactg
     2281 cataactata aaaaaaggca gtaagccgtg gccctaacag caattcataa ataaaggcca
     2341 gcacattggc cgcaattaaa agcaccgtca caatcggaaa actttgcgaa ggaacgttgt
     2401 cgcgtaacgg aatcacaggc ggggaccccc tcttccaaag taaccttcag cagcgaagaa
     2461 ccagagacaa gcaactccat tttatcgttg gcattgcaaa aatacaccca acctcggtac
     2521 ccaacctccg aaatatgcat attgttgtca tcattcctgc cttaaatgaa gccctttcaa
     2581 ttggaaaagt gctggacgat atcccgcgcc cacccgtgac agatgtcatt gtggttgaca
     2641 atgggagcac tgatggtacg ccagcgattg ctgccagcca cggggcacga gtcgtcaccg
     2701 agccacaacg ggggtacggt gccgcctgtt tgcgcggcct gagcgagatt ggccccgagg
     2761 ctgaaattat tgtttttctg gatggggatt attcagacta tccggaagaa atcagtgcct
     2821 taatcgaacc aatttgcagg gggaaggctg atctggtgat tggttcacgt gaactgggcg
     2881 accgcgaagc tggtgccctg atgccccagc agcggtttgg gaatgcgctg gccaccgggt
     2941 tgattcgatt gctctggggg catcgctata ccgatttggg accatttcgg gcaattcggc
     3001 gcaactcatt ggatcaattg catatgcagg accagaacta tggatggacg attgaaatgc
     3061 aaatcaaagc cattcgctat ggcctgcgaa ttcacgaaat tccagtcaga tatcgctgcc
     3121 ggattggcgt ttcgaaaatc agcggtacgg tgatggggag cttcaatgcc ggacgaaaaa
     3181 ttctatcgtg tattgcccgg ttctggtggg aagatcggtt tgggctgaag gctcggggtt
     3241 gaagaaaagg ggctgaagaa acctcgggct gaagaagtcg ggctgaagaa aatgaggaat
     3301 ttaacccaac ttcttcagcc ccgagcttgc gagtcttcag ccccaagccc tgagcccgtt
     3361 ttcttcagcc ccaagccctg agcccgtt
//
LOCUS       DAOQDO010000428         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21220_length_3386_cov_2.908136, whole genome shotgun sequence.
ACCESSION   DAOQDO010000428 DAOQDO010000000
VERSION     DAOQDO010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21220_length_3386_cov_2.908136"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..339
                     /locus_tag="PKZ53_08145"
     CDS             16..339
                     /locus_tag="PKZ53_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40445.1"
                     /translation="MVTSDKYFEPTNDGFRWVEPRSAQPVVPPLKLMPVSQLAIDQQG
                     NDLPHLMYQNNFGVEASFLARYPELVDFVRDINWNDHPIWYSVCGALALAEKAGWGKA
                     AISKT"
     gene            complement(343..2262)
                     /locus_tag="PKZ53_08150"
     CDS             complement(343..2262)
                     /locus_tag="PKZ53_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNJ40446.1"
                     /translation="MALLRTRRDAELASYFFFSPAKILGNAALREPQEEAYFALRRYF
                     SQAQKLPAMVEIPTGCGKTGIICIAPFRIARGRVLIVAPNITVRDTLVRSISWAENTS
                     FSERSNFYLKCKIFASPAELPRVTVLERGRTSLEDCLRADIVVANIQQLVGWLEVFDE
                     RFFDFIIVDEAHHVPAESWQKVALSFPAAKNVYLTATPFRSDSQPIRARSIYQYQLSR
                     AIQNEYIKNIVRVDAVASRLTFTLEGLTREFSFDEIAKLREEFWFSKGVALSEVCNQT
                     IVAHSIALLNEKRQDQIFHQLIAAACSIRHAQQLVALYCTAGLRATYVASQGMTREER
                     DARLRAYESGEFDCIVQVGILGEGYDHPNVSVAAVFRPYRSLSPYAQFVGRTLRRIDR
                     GNGSDNLAHIVGHVGLNLEPLWIEFKREARKQGHFRHSAEPQPERETLDEASETDDAN
                     LPTIIDQEIAGLDIDIFLANLSVQTGYREVTGGEVMAILTTLTHAASETDVPMMETVR
                     TQSSGDEPDVVPHILELRPDLERAKLRQRLNQEIRRAAGFILYELGLEDDGSLAAVLG
                     EHGEQTNYEVIIRALNREINHALHKDEDHSHREDWLQSELEAAFSQTRTLRDMLLFKI
                     RLVTNAHRQKKQKKV"
     gene            2673..>3386
                     /locus_tag="PKZ53_08155"
     CDS             2673..>3386
                     /locus_tag="PKZ53_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ40447.1"
                     /translation="MTTLPSNSSLPDQSLVPAPGKRMSAENRRRQIARVAMRLFAQKG
                     FTGTTTKEIAGQAGISEAIIFRHFATKQELYAAILDEKSIENEQDAFWLIMRDLASRK
                     DDQAFFQTLAQRILDRHRQDNTFMRLMLFSGLEGHELSEMFFSTHVQEVYSFLCGYIQ
                     QRIEDGAFQAVDPLLAARAFMGMAIHQSLTEQLFERNTADRPESRHLAEGFTKLFLNG
                     IATPVTQARNRKKIQVETKG"
BASE COUNT          808 a          859 c          876 g          843 t
ORIGIN      
        1 tgacgtgctt atcaattggt aacttctgac aaatattttg aaccgacgaa tgatggcttc
       61 cgctgggttg aaccacggtc tgcccaacct gttgtaccgc cactgaaact gatgccggtt
      121 tcccaactgg ccattgatca gcagggaaat gacttacctc atttgatgta ccaaaacaac
      181 tttggagttg aagccagttt cctcgcgcgc tatccagaac tggttgattt tgttcgagat
      241 atcaactgga atgaccatcc gatctggtat tcagtgtgtg gcgccctggc tttggcagag
      301 aaagctggat ggggaaaagc tgcgatcagc aaaacttgaa tcctaaactt tcttctgttt
      361 tttctgccgg tgcgcgttgg tcaccagtcg aattttaaac aacaacatat cgcgcaaggt
      421 tcgggtctgg gaaaaggcgg cttcgagttc tgattgcagc cagtcttcac gatgggaatg
      481 atcttcgtct ttgtgcagtg cgtggttgat ttcacggttc agtgcccgaa tgatgacttc
      541 ataattggtt tgttcaccgt gttcgccgag cactgccgcc agactgccgt catcttccaa
      601 acccagttca tagaggataa acccggctgc ccgacggatt tcctggttga gccgctggcg
      661 aagtttggcg cgctccaaat cgggtcgcaa ctccaggatg tgcggtacca cgtcaggctc
      721 atctccagat gattgggttc gaacagtttc catcattgga acatcagttt cggatgccgc
      781 gtgggtcaat gtcgtgagaa tagccataac ttcacctcca gtcacttccc gatagccggt
      841 ttgaacgctc aggttggcga gaaaaatatc aatgtccaat ccggcaattt cttgatcaat
      901 aatcgtgggc aggttggcgt cgtctgtttc gcttgcttcg tctaacgttt cgcgttcggg
      961 ttggggttcg gctgaatgtc gaaaatgccc ctgttttcgg gcttcgcgct taaattcgat
     1021 ccagagcggt tcgaggttga ggccaacgtg gccgacgatg tgggccagat tatcgctgcc
     1081 gttcccgcga tcaatacggc gcagtgttcg ccctacgaac tgggcatacg gggaaagcga
     1141 gcggtagggc cgaaacactg cggcaactga tacatttgga tggtcatagc cttcgcccag
     1201 gatgcccacc tggacaatac agtcaaactc gccgctttca taggctctga gccgggcgtc
     1261 gcgttcttcg cgcgtcatgc cctggctggc cacataggtt gcccgcagtc ccgccgtgca
     1321 atacaaggcc accagttgct gggcatggcg aatagagcag gccgccgcaa tgagttgatg
     1381 aaaaatctga tcctgtcgtt tttcatttaa caaggcaatg ctgtgcgcca caatcgtctg
     1441 attgcacact tcagacaggg ccacaccttt ggaaaaccag aactcttcgc gcagctttgc
     1501 aatttcatcg aatgaaaact cacgggtcag accttccagt gtaaaggtca accgcgaggc
     1561 gacggcatct acccggacaa tatttttgat gtactcgttc tggatggcgc gtgaaagctg
     1621 gtattgatag attgagcggg cgcgaatcgg ctggctgtcg ctgcgaaacg gcgtggcggt
     1681 gaggtacaca tttttggccg cagggaagga gagcgcgacc ttttgccacg attcagccgg
     1741 aacgtggtgg gcttcgtcaa caatgatgaa atcaaaaaat cgctcgtcaa atacctcaag
     1801 ccagccaacc agttgctgga tattggccac cacgatgtca gcccgcaggc aatcttcgag
     1861 gcttgtccgg ccacgttcga gcacagtaac tctgggtaat tcagccggtg acgcgaagat
     1921 tttgcacttt aagtaaaagt tgctgcgttc ggaaaaactc gtgttttccg cccaggaaat
     1981 cgaccgaacg agggtgtctc gaaccgtgat attgggcgcc acaatcaaga cccggccacg
     2041 ggcaattcga aatggagcaa tacagataat gccggttttc ccgcacccgg tgggaatttc
     2101 aaccatagcc ggcaacttct gagcctgact gaaataccgc cgcagggcaa aatacgcttc
     2161 ttcctggggt tcgcgcaggg cggcattgcc gagaattttg gccgggctga agaaaaaata
     2221 gctggccagt tcggcatccc gacgcgttcg caggagagcc atcgcagatt gagattctgg
     2281 agagggttcc ccagatgcct tttgaaattc gtccattttt ttctaactac ttataatcaa
     2341 aagagttcac catcgctatt gagtgtatgg agctacccca gtgtcagatt gtggagcgaa
     2401 gccggaggac tcccggtcgg gtcgctatgt gaggctgacg gttgggaaat aaaaatgaat
     2461 tgcagtgaag ctaagaaacc agaacaacta ttaggcgtgt atcactttga aatgaccaca
     2521 atatatctgg ttttaaccca ttacgccgta attccgcaag taaattctga agttccggga
     2581 acattttgtt gtgatttcag gtatccggcg ttgtttgtaa gtacttactt tgttatcgta
     2641 gccgaaattc ggtttcggag atcttcacct ttatgaccac gcttccatca aacagttcac
     2701 ttccggatca atcccttgtt ccagcccccg gaaaacgcat gtcggctgag aatcgccgtc
     2761 gccaaattgc tcgtgtcgca atgcgactct ttgcgcaaaa agggtttacc ggcacgacca
     2821 ccaaggaaat cgctggccag gccgggatca gtgaagccat tatctttcgc cactttgcca
     2881 ccaagcagga actctatgcc gccatcctgg atgaaaaatc tattgaaaat gaacaggatg
     2941 ccttctggct catcatgcgg gatcttgcca gtcgcaaaga tgatcaggcg tttttccaaa
     3001 cacttgccca gcgtattctg gaccgccatc gtcaggacaa cacctttatg cgtctgatgc
     3061 tctttagcgg tctggaaggc catgaactgt ccgaaatgtt tttttcgacc cacgttcaag
     3121 aggtgtattc ctttttatgc gggtatatcc agcaacggat tgaagatggc gcctttcagg
     3181 ccgtggaccc gctcctggcg gcacgcgcct ttatgggcat ggccatccat caatcactga
     3241 ccgaacaatt gtttgaacgc aacacggctg atcgtccaga gagtcgccac ctggctgaag
     3301 gctttaccaa attatttctc aacggaattg ccacaccagt gactcaggct cggaaccgaa
     3361 aaaaaattca ggttgaaacg aaagga
//
LOCUS       DAOQDO010000429         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21239_length_3385_cov_2.395495, whole genome shotgun sequence.
ACCESSION   DAOQDO010000429 DAOQDO010000000
VERSION     DAOQDO010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21239_length_3385_cov_2.395495"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PKZ53_08160"
     CDS             <1..1572
                     /locus_tag="PKZ53_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40448.1"
                     /translation="AGCSCPVGTGGHCKHVAALLLTWLAKPNEFLEIEELDTTLNRMT
                     KDELIALVKTFLSHAPDLESLVETPVQTASTQKGAPISPDVYRRKAAGAMKHSSRGGG
                     WYDDDESDYSENLAPILAAGTRFLNQKDYLNALSVYEGVMDESIDHYEEYEDESGSLG
                     DSIDDCIEGLAACLKGLENDSKARKHILQLLFTAHLADLNQGGVGFSDNAPPVILEQT
                     TADEKRFVADLVREKIGAGAQSWKNQALGRLLLNLEAETLDDETYLTICRETGQLKNL
                     ITRLLKLNRLKDAVQTAAASSDNELPVLVTLFIEHQHPQLADQLMEERAAKTNSFNIL
                     EWVRNRYLELNKLPEALEIAQRIFQNTPYMQHFKPVHQIAHKLGTWPAVRSKWIDWAK
                     SKKEFSLLIEIYLDDEDVENALATLKLHQQKPGSISYGRVFYGDRLAITVAKAAEATH
                     PREAIAIYKTQAEGFITARDRKNYAVAASYLKRVRDLYLELKAKKEWDIYITNLKEKN
                     RSLRALKDELASIGL"
     gene            1840..>3385
                     /locus_tag="PKZ53_08165"
     CDS             1840..>3385
                     /locus_tag="PKZ53_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010625850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNJ40449.1"
                     /translation="MATMQITDYHAKYFAHELTKRCSSESVEKLAGVLVDAQVDLNPH
                     QVEAALFAFRSPLSKGALLADEVGLGKTIEAGLVISQKWAERKRRILIITPSNLRKQW
                     HQELQEKFFLPCRILETKSYKDLVKQGETRPIDTRNFLTICSYQFARTKADEIQETRW
                     DLVVIDEAHRLRNVYKPSNIIANTLKRALKNSSKLLLTATPLQNSLLELYGLVSFIDE
                     HTFGDLKSFRDQFANLNDQRVFETLKSRLKPVCHRTLRRQVTAYVPYTRRHSIVQTFT
                     PQESEDQLYHLVSEYLQRENLQALPRSQRSLMTLVLRKLLASSTFAIAHTLGALSRRL
                     QTRTKHQPQPRSLEEELIDDYEALDETVDEWTEETTDIEPLTDDNQVAIEREIADLES
                     FRQLAVSITHNSKGDALLTALDRAFADAERLNAARKAIIFTESRQTQEYLLRLLSQSR
                     FSEGIVLFNGSNSDEKSKQIYQDWLKRHTGTDQITGSKTADMRSALVDYFREEGQIMI
                     ATEAGSE"
BASE COUNT          922 a          853 c          795 g          815 t
ORIGIN      
        1 gccggctgta gctgtccggt tgggactgga gggcattgca aacacgtagc cgctttgctt
       61 ctgacgtggc tggccaaacc gaatgaattt cttgagattg aggaacttga caccacgctc
      121 aaccggatga ccaaagacga gttgatcgcg ctggtcaaaa cctttttaag ccacgcgcca
      181 gaccttgaat cattggtgga aacgccggtt caaacagcat ctactcaaaa gggcgctccg
      241 atcagccctg acgtgtatcg tcgaaaagca gccggtgcaa tgaagcattc cagcaggggc
      301 ggcggttggt atgacgatga tgaatctgat tactccgaaa acctggcacc aatcctggcg
      361 gctggaaccc ggtttctcaa tcagaaagat tacctcaatg ccctttccgt gtatgaagga
      421 gtcatggacg aaagcattga ccattatgaa gagtacgaag atgagtccgg gagtctcggt
      481 gatagtattg acgattgtat tgaaggtctc gcggcctgcc tcaaaggtct tgaaaacgat
      541 tcaaaggctc gaaaacacat tctgcagctt ttatttactg cccatctggc cgatcttaat
      601 cagggcggag tcggctttag cgacaatgca ccacctgtga ttttggagca gacgaccgcc
      661 gatgaaaaac gatttgtcgc cgatctggtc cgcgaaaaaa ttggcgcggg cgcccaaagc
      721 tggaaaaacc aggcattggg cagactgttg ctcaacctgg aagccgagac gttggatgac
      781 gagacctacc tgaccatctg ccgcgaaacc gggcaattga agaatctgat cacccgattg
      841 ctcaagctca accgtttgaa agacgccgtc cagacagccg ctgcctcaag tgataacgaa
      901 ttgcctgtac ttgtcactct ttttatcgaa caccagcacc cgcaacttgc cgaccaactg
      961 atggaggagc gtgcggcaaa aaccaacagc ttcaatatcc tggaatgggt caggaatcgg
     1021 tacctggagt tgaacaagtt gcccgaagcc ctggaaatcg cccaacggat ttttcaaaac
     1081 actccgtata tgcagcactt taagccggtg catcaaatcg ctcacaagct tggtacctgg
     1141 ccggcagtcc gctcaaaatg gattgactgg gcaaagagca aaaaagagtt ttccctgctc
     1201 attgaaatct acctcgacga tgaggatgtt gaaaacgccc tcgcaaccct caaacttcac
     1261 caacagaaac ccggatcaat cagctatgga cgcgtttttt acggtgaccg tctggccatc
     1321 acagttgcca aggcagccga agcgacccat ccccgtgaag ccattgccat ctacaaaacc
     1381 caggcggaag ggtttattac agcccgtgac cgcaaaaact atgcagtcgc cgccagctat
     1441 ctgaagcggg tccgggatct ctatctcgaa ctgaaagcca agaaggagtg ggatatctat
     1501 atcaccaacc tcaaggagaa aaaccgcagc ctccgggcac tcaaagacga actggcatcc
     1561 attggtttat aggcagtgct ggacaattca atcgaatatc ttttgagttg attggctgta
     1621 atttcatgat cagccagaca acggactgcc tgatttttct gaactatttt ccccaccaat
     1681 cttgcaaaat tgattcaaac cggttatttg atttccggct tgacatttta ttgtcttgag
     1741 tcgtcttccc actttgggaa tagagagtta gagttttgcc ttcaactcct tgtccttcat
     1801 ttatttggtc cctttcttga agatttcagt acttaaccta tggctaccat gcaaatcact
     1861 gactatcacg ccaagtattt tgctcacgaa ttaacgaaac gttgctcttc tgagagtgtt
     1921 gaaaaactgg ctggggtttt ggtggatgct caggtggatc tcaacccgca tcaggtggaa
     1981 gcggcactgt ttgcctttcg gtcgccgtta tcaaagggcg cactcctggc tgatgaggta
     2041 ggtcttggaa aaaccatcga agcgggcctg gtgatctccc aaaagtgggc tgaacgaaag
     2101 cggcggattt tgatcatcac accgtcaaat cttcgcaaac aatggcatca ggagttgcag
     2161 gaaaagtttt ttctaccgtg tcgaatcctg gaaaccaaat cctacaaaga tcttgtcaaa
     2221 caaggtgaaa cccgtcctat cgacaccaga aactttctca caatctgttc ttatcagttt
     2281 gcacgcacaa aggcagatga gattcaggaa actcgatggg atttggtggt gattgacgaa
     2341 gctcaccgtc tacggaatgt ctacaaacca tccaacatca tcgccaacac attaaaacgg
     2401 gctttaaaaa actcatccaa gctactcctg acggcaactc cattgcaaaa ctcattgctt
     2461 gagctgtatg gcctggtcag ttttattgat gaacacacct ttggtgatct caagagcttt
     2521 cgtgaccagt ttgccaactt aaatgaccaa agagtttttg aaaccctcaa atcccgcctc
     2581 aagcctgttt gtcatcgcac ccttcgaaga caggttacag cgtatgttcc ttatacaagg
     2641 cgacattcca tcgtccaaac gttcacccca caggaaagtg aagatcagtt gtaccacctg
     2701 gtttcagaat atctgcagcg cgaaaacctg caggcattac cacggagcca aagatcgctg
     2761 atgaccctgg tgttacgcaa acttctggca tcatccacgt ttgcgattgc ccacacgctt
     2821 ggagcactca gccgccggtt acaaacgcgg accaagcacc aacctcagcc caggtcactt
     2881 gaggaagaat tgattgatga ctatgaagcc cttgatgaaa cggttgatga gtggacagaa
     2941 gaaacaactg atatagaacc acttaccgat gacaatcagg tggcaattga acgtgaaatt
     3001 gccgatcttg aatcttttcg tcagctcgcg gtttcgatta cccacaactc aaagggtgac
     3061 gccttgctca cagcccttga ccgggcgttt gccgatgctg agcgtctcaa tgccgcccga
     3121 aaagcaatta ttttcactga atcccggcaa actcaggagt atttacttcg gcttttgtcc
     3181 cagagccggt tttctgaagg gattgtcctt ttcaatgggt caaatagtga cgaaaaatcg
     3241 aagcagattt atcaggattg gctcaagcgc cacacgggaa ctgaccaaat cactggatct
     3301 aaaacggctg atatgcgctc agcattggtg gattattttc gtgaagaagg ccaaattatg
     3361 attgccactg aggctggatc tgaag
//
LOCUS       DAOQDO010000430         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21305_length_3379_cov_2.982250, whole genome shotgun sequence.
ACCESSION   DAOQDO010000430 DAOQDO010000000
VERSION     DAOQDO010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21305_length_3379_cov_2.982250"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1173
                     /locus_tag="PKZ53_08170"
     CDS             124..1173
                     /locus_tag="PKZ53_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ40450.1"
                     /translation="MRKNLFVAMVSVAMVGSLALTGYAVKLVEPGKAFEAATTTTEKS
                     PANLVPGSGRIEPRSEEIKISSQVSGKLKSVLVEEGDLIEKGQVLAELENADFVALVA
                     SAQARLRIREAELLKLKNGSRTEERREAQANVREAEAQVANARLEYERKQKLLAEGFI
                     ARHDVDTLDRNYQVAQARLEALRERMNLIETQTRTEDLARAEAEVQLAQAEVQEAQAR
                     LAKTIIRSPISGIVLRKHLHIGESVSDTNPNPIVTISDRLAQRVRVDIDESDVAKVKL
                     GQKAYVTADAFGEKQFYGKVVQVGQILGKKNIRTEEPTERVDTKILEVLIELEPDTRL
                     PLGLRVNAFLIAENQ"
     gene            complement(1263..1727)
                     /locus_tag="PKZ53_08175"
     CDS             complement(1263..1727)
                     /locus_tag="PKZ53_08175"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ40451.1"
                     /translation="MAMPYFYEVEVKWDREKIGNLKVEGLPDLSVATPPEFGGHEGYW
                     SPEHLFVASVNSCFMATFLAMAGNSKLEFESFDSTATGKLEKVEGVGLQITEISIRPV
                     LVISNTKDLERGMKLLEKAEKYCLISNSIKSRITLEPVILTEHVPVLNGELF"
     gene            complement(2069..>3379)
                     /locus_tag="PKZ53_08180"
     CDS             complement(2069..>3379)
                     /locus_tag="PKZ53_08180"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNJ40452.1"
                     /translation="RFGGNGSDFGLGITADTNGNAYITGNTSGSKTNFPLTANAYQTN
                     GPIDSNSHVFVAKIAANAGPNGVLYCSYLGDTRPNNVSTGATLVRPSGARGSRKIIAY
                     GDAGDEPGAFIYVTGYTNTPNLKITSGAIQNTFGGGNDIFVARLYINNPNAASIVTYL
                     GGDGDEYALDMVVDSNHQIYLTGYNYRGGTSKVAFPTTSNAYQQTQQGPTDAIVVKIS
                     TNVAGGNTLAYSSLLGGMGTDSAHALAVDSNGLIYITGEAGYPSFPTTANAFKTTYTN
                     ADAFVSQIDPSQSGSASLVYSSLLGGNGLDTGYDIALGGRESNPYVYILGQTYSNNAT
                     FPAKDAQAPVNTHVGNSDIFVARFDLLNSGANSLTHVAFIGGTAFDGVFRTEHITTFA
                     IGVDSVGYAYFSGHSVSTNYPVVNPFDSGNSNSSYEAVVGKLTF"
BASE COUNT          884 a          818 c          840 g          837 t
ORIGIN      
        1 attcgcaagc tcagggctga agacttaggg ttaaagaaat cttgtatttt gaccccaagc
       61 ccactttctt cagccccagg ccctgagctt gcgaatcttc aacccaagat ttaggagaag
      121 agtatgcgaa agaatctatt cgtggcgatg gtttcagtgg cgatggtcgg cagtctggcc
      181 ttaaccggat atgctgtgaa actggttgaa cctggaaaag cttttgaagc cgcgacgacc
      241 accacagaaa aatcacctgc caatctcgtt ccaggttcgg ggcggattga accgcgttct
      301 gaagaaatca aaatcagttc gcaggtcagc ggaaaattga aatccgtgct ggtcgaagaa
      361 ggtgatttga tcgaaaaagg tcaggtgctg gctgaacttg agaacgctga ttttgtggcg
      421 ctggtggctt cggcccaggc ccggctccgc attcgcgaag ccgaattgct caagctcaaa
      481 aatggttcgc gaaccgaaga gcgccgcgaa gcccaggcca atgttcgcga agccgaagct
      541 caggttgcga atgccagact tgaatacgaa cgcaaacaaa agctcctggc cgaagggttt
      601 attgcgcgcc acgacgtaga cacactggat cgaaattacc aggtggccca ggcccggctc
      661 gaagcccttc gcgaacgaat gaacctgatt gaaacccaaa ctcggactga agacctggca
      721 cgggccgagg ctgaagtcca actggctcag gctgaagtgc aggaagccca ggcccggctg
      781 gccaaaacga ttattcgctc gccgatttcc ggaatcgttc ttcgcaaaca tctgcacatt
      841 ggtgaaagtg tttccgacac caacccaaat ccgattgtga ccatttcaga tcgactggcc
      901 cagcgtgttc gggttgatat tgatgaatct gacgtggcca aagtcaaact gggacaaaaa
      961 gcctatgtca ccgctgatgc gtttggagaa aaacaatttt acgggaaagt cgtccaggtt
     1021 gggcaaatct taggcaagaa aaacattcgg accgaagaac caaccgagcg cgtggatacc
     1081 aaaatcctcg aagtcctgat tgaactcgaa ccagacaccc ggttgcccct gggattgcgg
     1141 gtcaatgcct ttctcatcgc agaaaatcaa tagtctcacc acagaggcca cagaggatcg
     1201 cagaggcttc aaaaatgggg gttcctctgt gttcctctgt ggcctctgtg gtgaaaaatt
     1261 cattagaata attcgccgtt taaaactgga acatgttcag tcaaaatcac tggttcgagt
     1321 gtgattcgag atttgatcga atttgaaatc aggcagtatt tttcggcctt ctcaagcaat
     1381 ttcatccctc gctccaggtc tttggtgttg gagatgacca gcacaggccg gattgaaatt
     1441 tcggtaattt gaagaccgac gccttccact ttttccagct ttccggtcgc ggttgaatca
     1501 aagctttcga actcaagttt ggaatttccc gccatggcca gaaacgtggc cataaaacac
     1561 gaattgaccg acgccacaaa taaatgctct gggctccagt agccttcatg cccaccaaat
     1621 tctggcggcg tggcaacact caaatctggc agcccttcga ctttcaggtt tccaatcttt
     1681 tcccgatccc atttcacttc aacttcataa aaatatggca tggccatagt gctcagcccc
     1741 tttctgaaat ttttgtcttc agactcaagt gcaagttttg tgccagagga atttttctca
     1801 gaagggtgtc tggggggaac gttcactgct ggcggtcgag ccccagggaa cacaaggaca
     1861 caaaaggcat ctcgccgccc gcagtggaat attgatttga ttggaaagtg ttgccgaaaa
     1921 gatgcttttc ctttaccgac cttcgcccca gttgaatatc cagttggggt tgatgtgaaa
     1981 aaattcacac cggacgttaa gtaaaaaaca aaagacctgc ggcagttgcc tgcggcaggt
     2041 ctgaagacgt ggggttctgg ttaagcgatt agaatgtcaa ttttccaacc accgcttcat
     2101 aactgctatt gctatttcca gaatcaaacg gattgaccac cgggtaattg gttgatacgg
     2161 aatgacctga gaaataagca tacccaacac tgtcaacccc aatggcaaaa gtggtgatgt
     2221 gctcagttct aaaaacccca tcgaaggcag ttccaccgat aaatgcgacg tgggtgagcg
     2281 aattggcacc actgttgagc agatcaaacc gggcgacaaa aatgtcagag tttccaacgt
     2341 gagtatttac tggtgcctgc gcatctttgg caggaaacgt cgcattattt gagtaggttt
     2401 gccccagaat atagacataa gggttgcttt ccctgccgcc taacgcaata tcgtatcctg
     2461 tgtcgaggcc gttgccacct aataaacttg aatataccag tgacgctgag cctgattgac
     2521 ttggatcaat ttggctcaca aatgcgtctg cattggtata agttgtttta aatgcattgg
     2581 cagtggttgg gaaagatgga tacccggctt ctccggtaat atagatcaac ccatttgaat
     2641 caactgcaag tgcgtgcgcg ctgtcagtcc ccatacctcc aagcaggctc gaataggcca
     2701 gggtgttgcc gccagccaca ttggtgctga ttttgacgac gatggcatca gttggacctt
     2761 gttgggtttg ctggtaagca ttgctggtgg tggggaacgc aactttggat gttccccctc
     2821 gatagttata tccggttaaa tagatctggt gattggaatc caccaccata tcaagagcat
     2881 attcatcacc atctccaccc aggtaagtga caattgaagc tgcatttggg ttgttgatat
     2941 agagcctggc cacaaaaatg tcgtttccgc caccaaaggt gttttggatt gccccactgg
     3001 tgattttcag gttgggggta ttcgtatagc cagtcacata gataaaggca ccgggttcat
     3061 cgccagcatc gccataggca ataatttttc gacttccacg ggctccagag ggcctgacca
     3121 gagtggcccc agttgataca ttatttggtc gagtatcacc cagataactg caatataaga
     3181 ccccatttgg accggcattg gcagcgatct tggcgacaaa aacgtgggag ttgctatcga
     3241 ttgggccatt tgtttggtac gcattggcag tcaacgggaa attggttttt gatccacttg
     3301 tattcccggt aatgtaggcg ttcccatttg tatcagccgt aattcctaat ccaaaatcac
     3361 tgccgtttcc accaaatcg
//
LOCUS       DAOQDO010000431         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21343_length_3375_cov_3.071988, whole genome shotgun sequence.
ACCESSION   DAOQDO010000431 DAOQDO010000000
VERSION     DAOQDO010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21343_length_3375_cov_3.071988"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKZ53_08185"
     CDS             complement(<1..317)
                     /locus_tag="PKZ53_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ40453.1"
                     /translation="MDHVTPTSFSSPVLQPQERILLREALYRRYATSSSGIGGTARWV
                     RFFWKRNAWRLVIGLTRAIKRLVDLVVSTTLLIGLAPLFAAVALAIKLTDGGPALFWQ
                     TRVG"
     gene            complement(329..895)
                     /locus_tag="PKZ53_08190"
     CDS             complement(329..895)
                     /locus_tag="PKZ53_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNJ40454.1"
                     /translation="MFQNLREDWQTYEGDPWRQGLWVMVVYRFGNWRYQIKPRLLRAP
                     LSFVYKLFKVLSQILTGIDLPCEARVGKRFKIEHFGGIIISGDAQFGDDCVIRNGVTV
                     GLRRTGTRGAPKIGDRVDIGAGAVILGDIVIGHDVAIGANAVVLTSIPPDSIAVGVPA
                     QIRPRKKVSTLPQALDETASRVVAGVGQ"
     gene            complement(1081..2076)
                     /locus_tag="PKZ53_08195"
     CDS             complement(1081..2076)
                     /locus_tag="PKZ53_08195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ40455.1"
                     /translation="MPFTIFPDASIIIVSFNTRDLLRDCLRTVKETCSGLSYEIIVVD
                     NASRDGSAAMVEQEFPEVRLIHSPINLGFAAANNRGFEIAQGRYFILLNSDAFLKPGA
                     LKTAIQHMDESPLVGLAGGRLVGRDGDWQPSARMFPSIVNDFLSISGLSARFPKSRLF
                     GRVDRTWADENQSTEVDWVPGAFSIISRDVLAEIGFFDERFFLYYEEVDLCKRIKHAG
                     YKIWYWPDIEVVHLGGESSKTVKHLTMSKSGAQLTLWRMRSALLYYRKHHGLMGAWLE
                     RLLETSWHKIRAWKNSGTENADSLAKAADSQNLLSLMRQAWQETQGGRLSPPRPW"
     gene            complement(2211..3374)
                     /locus_tag="PKZ53_08200"
     CDS             complement(2211..3374)
                     /locus_tag="PKZ53_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ40456.1"
                     /translation="MLIVTLPGTIELLLLTVGAIVGKFTTARYPNQETPTTSLLKLGV
                     VIPAHNEAGGIAACVKSVLACQAAGVDLQVVVIADNCTDMTAQFAKEAGARVIVRMDQ
                     ANRGKGFALDVAFGRLLREGIEAFVVIDADTEVEPNFITEMAHAFQQGADAVQCRYLV
                     NNAQFTFRTRLMNVALLAFNVLRPLGRNAIGCSAGIFGNGFGLSATTLCEVPYSARSV
                     VEDLEYHLKLVKAGKQVRFLNSTTVRAEMPAGGRAAQTQRSRWEGGRIRMMLDFAPSL
                     IRDIARGERRLIEPLLDLLLLPLAFHVVLLASLMLVPNPFLKIYAASSLALVLFHVLS
                     AVWVGEGTKNDVLALLGAPLYILWKMTMVRSVVRSSRKKAEWVRTERAAPNQS"
BASE COUNT          828 a          930 c          871 g          746 t
ORIGIN      
        1 ccaaccctgg tttgccagaa cagagccggc ccaccatccg tcaatttgat ggccagtgcc
       61 acggcggcaa acaacggcgc cagtccaatc aggagcgttg tggaaaccac cagatcaacc
      121 agtcgtttga tggctcgggt caggccaatg accagccgcc aggcgttgcg cttccaaaaa
      181 aaacgaaccc agcgggcggt tcctccaatg cctgaggaag aggttgcata tcgccgatac
      241 aacgcctcgc gtaacaaaat ccgttcctgg ggttgaagca ccggtgatga aaagctggtc
      301 ggggtaacgt gatccatagt tttttttcct actgcccaac cccagccacc acccgtgatg
      361 ctgtttcatc gagtgcctga ggcaacgtgg agactttttt ccggggccga atctgagccg
      421 gaaccccaac ggcaatggaa tccgggggaa tgctggttaa aacgacggca ttggcgccaa
      481 tggccacgtc gtgtccaatg acgatgtccc ccaaaattac cgcgccggca ccaatatcaa
      541 cccggtctcc gattttgggg gcgcctcggg tcccggtccg gcgcaaccca acggtcacgc
      601 catttcgaat cacacaatca tctccgaact gggcatctcc gctgatgatg attccaccga
      661 aatgttcaat tttaaagcgt ttcccaactc tggcttcaca gggtaaatca atgccggtca
      721 gaatctggct cagaactttg aacagtttgt atacaaatga aaggggggca cgaagcaagc
      781 ggggtttgat ttggtagcgc cagtttccaa accggtagac caccatcacc cacaggccct
      841 gccgccaggg gtcgccttca taggtttgcc agtcttcacg taagttttga aacatacaag
      901 tcaatgcact tcaggggttg ggtttacgaa atcgtcaaac cattgaacat tcactgtgat
      961 cctgttttgt ttgtatgaca ccggttgcga atgaccctgt cggatgaacc agaagctaag
     1021 ttgttgataa aatggtagtt ccttcagccc atagcccggt atcttcagcc ctaaatgata
     1081 ttaccagggg cgtggaggag agagccgccc tccctgtgtc tcttgccaag cttgccgcat
     1141 caaggaaaga aggttttggg agtcggcggc cttggctaat gaatctgcat tttccgtacc
     1201 agaatttttc caggcccgga ttttatgcca ggaagtttcc aaaagtcgct ctagccacgc
     1261 tcccatcagg ccatgatgct ttcggtaata taacaatgcg ctgcgcatcc tccagagggt
     1321 caactgtgct ccggattttg acatcgtcaa atgtttgacg gtttttgacg attctccacc
     1381 aagatggaca acctcaatat ctggccagta ccagatcttg tatccagcgt gtttaatgcg
     1441 cttacacagg tcaacctctt cgtagtagag gaaaaaccgc tcgtcaaaga aaccaatttc
     1501 ggccaggacg tcgcggctaa tgatcgaaaa cgcgcccggt acccagtcaa cctcagtgga
     1561 ttgattttca tcagcccagg ttcggtcaac ccggccaaac aatcgggatt taggaaaccg
     1621 ggcggaaagt cccgagatgc tcaaaaaatc gttgacaatc gaggggaaca tccgggctga
     1681 cggctgccag tcgccatcac gtcccaccag ccgcccccct gccagcccaa ccagaggaga
     1741 ttcatccata tgctgaattg cggttttgag ggcgcccggt ttgagaaacg catcagaatt
     1801 gagaaggata aaatagcgtc cctgggcaat ttcaaatccg cgattgttgg ccgcggcaaa
     1861 ccccaaattg atcgggctgt gaatcaaccg aacttcggga aattcctgtt cgaccatcgc
     1921 cgccgagcca tctcgcgacg cattgtcaac cacaataatt tcataggaca ggcccgagca
     1981 ggtttcttta actgttcgca gacaatcccg caacaaatca cgggtgttaa atgaaacaat
     2041 gataattgaa gcatctggga agattgtaaa tggcataggc ggcaccagcg ttgtgggagg
     2101 ttgtcgtttt gaggtcaaga tgcggcgtcg aaggccgaag attggggaac gaaaaagcaa
     2161 cgcaagccaa cgcggccctg tggttttccc ctcagctcca gccctggttt ttagctctgg
     2221 tttggggcgg cccgttcagt ccgaacccat tcggctttct tgcgtgagga tcgaaccaca
     2281 gatcgaacca tggtcatttt ccacaagata tagagtggcg ccccaagcag cgcgagcaca
     2341 tcatttttgg ttccttcgcc aacccatacc gcacttagca catggaagag aaccagggcc
     2401 aggcttgatg ccgcatatat tttcaagaat ggattgggaa ccaacatcaa gctcgcgagc
     2461 agcacgacgt gaaacgccag ggggagcagc agcaggtcca ataacggctc gatcaatcgt
     2521 ctctcacctc tggcgatgtc gcggatgaga ctgggggcaa agtcaagcat catccgaatc
     2581 cgaccacctt cccagcgtga gcgctgggtt tgggccgccc gcccgcccgc tggcatttcc
     2641 gctcgcacgg ttgtggagtt gagaaacctg acctgtttgc ctgctttgac cagtttcagg
     2701 tgatattcga gatcttcaac cacggatcgg gctgagtagg ggacttcaca caaagtggtg
     2761 gcgctgagcc caaatccgtt gccaaaaatt ccggctgaac acccaatggc gttccgaccc
     2821 agtgggcgca agacattaaa tgccagaagc gccacattca tcaggcgggt tcgaaacgtg
     2881 aactgtgcat tgttgaccag ataccggcat tgaacggcat cggcaccctg ttgaaatgcg
     2941 tgggccattt cagtgataaa atttggctcg acttcagtgt cggcatcaat caccacaaac
     3001 gcctcgattc cttcccggag caatcgtcca aaggccacat cgagggcaaa tcctttgcca
     3061 cggttggcct ggtccattcg cacgatcacc cgggcgccgg cttctttggc aaactgggcc
     3121 gtcatatccg tgcaattatc tgcaatgaca acgacttgca ggtcaactcc ggcagcctgg
     3181 cacgctaaca cactcttgac acaggcggcg attcctccag cctcattatg ggccgggatc
     3241 acgactccca gtttgagcag agaggtggtg ggtgtttcct ggtttggata tcgagcggtc
     3301 gtgaactttc caacgatggc acccactgtc agcaggagca attcaatcgt tccaggaagc
     3361 gttacaatca gaatg
//
LOCUS       DAOQDO010000432         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21453_length_3366_cov_2.945334, whole genome shotgun sequence.
ACCESSION   DAOQDO010000432 DAOQDO010000000
VERSION     DAOQDO010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21453_length_3366_cov_2.945334"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKZ53_08205"
     CDS             complement(<1..531)
                     /locus_tag="PKZ53_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ40457.1"
                     /translation="MIRFTSNHSDLSNSNGFQFKFYCDKCHNGHLTRFQNSMGGTAEG
                     LLRAAGSLFGGVFHQAANSVDYLQNTFRGTEHDRAFEIAVQEAQQYFHQCQRCGKWVC
                     PEVCWNPQYGICKDCAPAFEEERAAARAQGAQSQLFRQGRWGTVLATPSDLQNPIPAT
                     CSHCNAPTTGTRASSSP"
     gene            954..1394
                     /locus_tag="PKZ53_08210"
     CDS             954..1394
                     /locus_tag="PKZ53_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNJ40458.1"
                     /translation="MKAQKYIKLSKLELEMMDVIWDLERASVREIQEALPEHKRPAYT
                     TVQTIVNRLEEKGAVTRTKKIGNAFIFEPAISRKAAHRRLFDDVLEMLGGSVQPLMAH
                     LVETGKLTLEDVRKLESTLTKLESSTSSELESSTSSEPSQPSKE"
     gene            1401..3314
                     /locus_tag="PKZ53_08215"
     CDS             1401..3314
                     /locus_tag="PKZ53_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNJ40459.1"
                     /translation="MTFLEWLNTVSQTAWPAVVNHLWQTLLFVGLAWGVVKVLKHGPA
                     RLRFFVWSLAAVKLAVPAILGALLLQQVGVHAPEILTPVAQIVKTSVITVSSTPEVMI
                     NELVVIQGTRPGVQWLNTPLFCTFVWFGGMVFLLSRWLMKRASFLRVLHQAVVCSGER
                     ELKALEGAKARLGFEKDVRLVLSRHIPEPGVWGVLNPVVILPDTLSQHLSDQELEHVL
                     MHELTHITRYDNLIANVNMVLCCVFWFFPVGWWISKAMLHEREQACDERVLEVSTSTN
                     SYAAGMLKVFQFGLQWRELAGVSYATGSDLHRRIENIMENPIRPLNSFWSRVALGTLV
                     AGFGLILVGTETWGNSLVIQNQKTPKAGVRMTPPPAPPMPPAPPAPPVPTDGPPAPPE
                     TPEAPEAPEPPPMPDDETLPALAPTPEAPPAATPAPATAPRPATPPSAQAPTPPPAPA
                     APTPQAIKKVQPVYPPLARSARVQGDVTVETTVSETGDVISARAVSGHALLQQAALTA
                     ARQWKFEPAVYNGQPAKFVSQINFEFKLPTQEEDGQVIRRSEGVIRGNAINRAVPEFP
                     EQAKIDGVEGDVTLSIKINEDGQVYSVEVLSGPKVFWDACLKAASQWKFNPTVIEGQP
                     VKVMGQLTFRFKL"
BASE COUNT          829 a          941 c          838 g          758 t
ORIGIN      
        1 aggggagctg gacgcccggg tgccggtcgt cggtgcatta caatgcgaac aggttgctgg
       61 aatggggttc tggagatcgc tcggcgtagc cagcacggtt ccccaccgtc cctgacgaaa
      121 caactggctt tgcgcccctt gagcacgagc ggcggcgcgt tcttcttcaa aagccggagc
      181 gcaatcttta cagatgccat attgtggatt ccagcacact tccgggcaca cccattttcc
      241 acaccgctga cattggtgga aatactgctg ggcttcctgg accgcaatct caaacgcgcg
      301 gtcatgctcg gtaccacgaa atgtattttg caaataatcc acactgtttg ccgcctgatg
      361 aaaaacccct ccgaacaaac tccccgccgc ccgcaacagc ccttccgctg tgccgcccat
      421 cgaattttga aaccgggtga ggtgcccgtt atggcacttg tcacaataaa acttgaactg
      481 aaaaccgttg ctgttcgaaa gatcactgtg gtttgacgtg aagcggatca tgagaaatct
      541 ccctgcccaa ataaatgcct gtgatgaagc tggtttgatg aaccttttaa ctgtagcaaa
      601 aatccccgac tgaaaaaaat ccaacaactc aactctccgg ctgaaatttc agattctgag
      661 ccagaatcct tttaaaaaaa ttgcttgacc atttactaca tttgtcgtag attctccggc
      721 gttcgtagta agtttatttt caaatcatca catcaggctg ggaaaagctt tacgggcaaa
      781 agatgtgtct tcccgtaacc caatttcttc agccccattt tagggctcag ggtttagggc
      841 tgaagactcg caagctcagg gctgaagaaa tcgggttcaa tagcctgagc ccgaagtctt
      901 cagcccgagg tcttcagccc gatttcttca accctgaatt cggaggttcg cccgtgaaag
      961 ctcaaaaata catcaagctc tcaaagcttg aacttgaaat gatggacgtc atttgggatc
     1021 tggagcgtgc ttcagtgcgc gaaatccagg aagccctgcc ggaacacaaa cgcccggcct
     1081 acacaaccgt ccaaaccatc gtcaaccgac tggaagaaaa aggcgctgtg acgcgcacca
     1141 aaaaaatcgg caatgccttc atctttgaac cggcaatttc acgtaaagcg gcgcaccgac
     1201 gattgtttga cgatgttttg gaaatgctgg gcggctcggt ccaaccactg atggcgcatc
     1261 tggtcgaaac tggcaagctc acactcgaag acgtccgcaa acttgaatca accctcacca
     1321 aacttgagtc cagcaccagt tcagaacttg agtccagcac cagttcagag ccttcacaac
     1381 cttcaaagga gtagccggat atgacttttc tggagtggct gaataccgtt tcgcaaactg
     1441 cctggccggc agtcgtcaat cacctctggc aaacacttct ttttgttggg ctggcctggg
     1501 gcgtggtgaa agtattgaaa catggccccg cccgactgcg atttttcgtc tggtcgctgg
     1561 cagcggtcaa actggcagtc ccagccattt tgggggccct gctgctgcaa caggttgggg
     1621 ttcatgcccc ggaaatcctc actccagttg cccaaattgt caaaacttca gtgattaccg
     1681 tcagttcaac ccctgaagtt atgattaacg aactggttgt gattcaggga acccgacccg
     1741 gagtccagtg gctcaatact ccgctcttct gcacgtttgt ctggtttggc ggcatggtct
     1801 tcctgctcag tcggtggctg atgaagcgcg cttcattcct gcgagtgttg catcaggcgg
     1861 ttgtctgcag cggcgaacgg gaactcaaag ccctcgaagg tgccaaagcc cgtcttggtt
     1921 ttgaaaaaga cgtgcgactg gtgctctccc gccatattcc cgaacccggc gtctggggcg
     1981 tactgaatcc agtcgttatt ttgccggata cactctcgca acatctttcc gatcaagaac
     2041 ttgaacacgt gttgatgcac gaactaaccc atatcacccg gtatgacaat ttgatcgcga
     2101 atgtgaacat ggtgctctgt tgtgtgttct ggttcttccc cgtgggctgg tggatttcaa
     2161 aagccatgct ccacgaacgc gaacaggcgt gtgatgaacg tgtcctcgaa gtcagtacca
     2221 gcaccaactc gtatgcggct ggcatgctga aagtttttca atttggcctt caatggcgtg
     2281 aactggctgg ggtgtcatat gccaccggct ctgacctgca caggagaatc gaaaatatta
     2341 tggaaaatcc gatccgtccg ttgaattcat tctggtcacg tgtcgcgctg ggcacgctgg
     2401 tcgctggctt tgggttaatc ctggttggga ctgaaacctg gggcaattca ctcgtcattc
     2461 aaaatcaaaa gacacccaaa gccggtgtgc gaatgacccc accaccagca ccaccgatgc
     2521 caccggcacc accggcacct ccagtcccaa ctgatgggcc acccgcgcca cccgaaactc
     2581 cggaagctcc tgaagcccct gaaccaccgc cgatgccgga tgacgaaacg ctgccagccc
     2641 tggcaccaac tccagaagct ccgccagccg caaccccggc tccagcaacc gcaccacgcc
     2701 cggcgactcc accatcagcc caggcaccaa caccgccacc agctccggcg gcaccaaccc
     2761 cccaggcaat taagaaagtt cagccagtgt atccgccact ggccagatct gcccgggtac
     2821 agggtgatgt cacagttgaa actacagttt cagaaacggg tgatgtcata agtgccagag
     2881 cggtcagcgg tcatgccctt ctgcaacagg cagcacttac cgccgctcgt cagtggaaat
     2941 ttgaaccagc cgtttataat gggcaaccag ccaaatttgt gagccagatc aattttgagt
     3001 ttaaactccc aactcaggaa gaagatggcc aggtaattcg tcgctcagaa ggggtaatcc
     3061 gtggcaacgc tattaaccgt gcagtaccgg aattcccaga acaagccaaa attgatggtg
     3121 ttgaaggcga tgtcacgctg agcatcaaaa tcaacgaaga cggtcaggtg tactcagtgg
     3181 aagtactttc aggcccgaag gtcttctggg atgcctgcct caaagccgcc agccagtgga
     3241 aattcaaccc aactgtgatt gaaggccagc cagtgaaagt gatgggccag ttgacgttcc
     3301 gatttaagct ctaataccaa ttagggttca gggtatcggg ttcagggttg cggggaaata
     3361 caattt
//
LOCUS       DAOQDO010000433         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21564_length_3355_cov_3.511515, whole genome shotgun sequence.
ACCESSION   DAOQDO010000433 DAOQDO010000000
VERSION     DAOQDO010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21564_length_3355_cov_3.511515"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..801
                     /locus_tag="PKZ53_08220"
     CDS             79..801
                     /locus_tag="PKZ53_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40460.1"
                     /translation="MLPKFAHKFLIRRAPDFYDWGSGYFEFPMNAEVLKTEAHPYLVE
                     RDFEEYEKLTPQERKQKVLEIEALLDESRLAPEVQANLLVEQGRLLWASQKYNQSCNN
                     FDRATAIHPHNHKAWVNRGAALDNLGRLEEAIASYDQALTVKFDEYNVWCYRGNALYN
                     LGRLEEAIASYDRILAMKPDNPQAWNNRGAALEDLGRVGEALASYNQAIALNPDKLLY
                     RKNKERLLAQIQDSHTLKQTAE"
     gene            1030..>3355
                     /locus_tag="PKZ53_08225"
     CDS             1030..>3355
                     /locus_tag="PKZ53_08225"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40461.1"
                     /translation="MLWMALARHFGWLWLIGLIGTGLSGIALAQMPVRPAQAGMRFVR
                     NYTTDEYKAADQNWAIVQDPRGVMYFGNGRGILEYDGVTWRLTPLANRSTVRSLAVDA
                     QGHVFAGGINDFGYLTPDDKGQQQFVSLVEKLPEDARHFADVWRTYSTPNDVVFQTVN
                     YVFLWNGQTLDVVQPAPSGGTFSACLQVNQDLYLVQRSLGLFKLVNRALQPVPGGQWM
                     VEKRLSGLLPMSDGKLLAVVRDEGLFVYDGQTFTPFAAPEVSQALVAWDPYKAMVLPD
                     GRFAFATITHGAVILRPDGTLDEIIDKKAGLQNQQVKNILADRDGNLWMGLDNGIARA
                     EVISPFTVFDDRCGIEDVVQAFIEHAGKYYLSTANGAFVWDPQVTQPSGAPLFQYISG
                     PKTSCFDFGIAGNDLFVSADNGVFQLEGTKATKVLDSLGTAVSFSFCTSKRYPNRLYV
                     GQQYGLAVLERRGSSWVSAGKVPGIDTEIRSIIELSDGTLWLGTHAEGILRVEFTSLS
                     APESFNPATVHVSIRRFTAQDGLPMMSRIRLMRISDRLLFLSQNGMVEFDEARGRFFP
                     STFLSDPTLPPLRRLVSDSQGTLWGYFEDNTCARLTPVAGRYQVDTITLKRMPGAIYN
                     WVFVDSHNTIWFGGSKGLVRIDPQRIKPPPSPVPVLIRKVLAGNKRDLMFGGATSATM
                     PVLHLPYTSNQVRIEYALPTFIQEQSIRYQYWLEGVDANWSELTTETIKEYTNLHEGQ
                     YHIRIRAEDALGYQSLETVYAVQILPPWYRTWWAY"
BASE COUNT          809 a          845 c          848 g          853 t
ORIGIN      
        1 tcattaccac cgttgctggg tcatctgaat ttgcaacggg aacggttccg ggaccatttc
       61 aacttctgct ttgttttttt gcttccgaaa tttgcccata aattcctcat ccgtcgcgct
      121 cctgatttct acgactgggg ttccgggtat tttgaattcc caatgaatgc agaagtgttg
      181 aaaaccgaag cacaccccta tctggttgaa cgagattttg aagagtatga aaaactaacc
      241 ccacaagagc gcaagcagaa agtcttagaa atcgaagcac tccttgatga atccaggttg
      301 gcaccagaag ttcaggcaaa tttgctggtt gagcagggac gattgctttg ggcttcacaa
      361 aagtataatc aaagttgtaa caactttgat cgggcaacag caattcaccc tcataaccac
      421 aaagcctggg ttaatcgcgg agctgcattg gacaatttag gccgtttgga agaagccatc
      481 gctagttatg atcaggccct gaccgttaag tttgatgagt acaacgtctg gtgctatcgc
      541 ggtaatgctt tgtataactt agggcgactc gaagaagcta tcgctagtta tgatcgtatc
      601 ctggctatga agcctgataa tccccaagcc tggaacaacc ggggtgcagc cttagaagat
      661 ttaggacggg ttggagaagc tctcgccagc tataaccagg cgattgccct caatccagat
      721 aaattgctct accggaaaaa taaagaacga cttcttgctc aaattcaaga ttcccacact
      781 ctgaaacaaa ctgctgagta attttccctc aaaactccct tcctgaagaa aaaccgcctg
      841 aaggcagcaa ctcttcactt tcgtgctaca ctgcgccgtc tcttgttcac gaaacacaag
      901 acataacgtg atgataccag tttgaactga tttcattgga tgatgagaag gctatctctt
      961 tgaaactgaa cggcctcctt catacttcag actttagact tcagacctgg caggaggagc
     1021 ttccccccta tgctctggat ggctctggcg cggcatttcg gatggctgtg gttgattggg
     1081 ttgattggca ctggcctgtc aggaattgcc ctggctcaga tgccggttcg accagctcag
     1141 gccgggatgc ggtttgtgcg taactacacc accgacgaat acaaggccgc tgaccagaac
     1201 tgggcgattg tccaggaccc acgcggagtg atgtacttcg gaaatggtcg gggcatactc
     1261 gaatatgacg gtgtcacctg gcggttgaca ccgctcgcta accgttccac agttcggtca
     1321 cttgcggttg atgctcaagg ccacgtcttc gctggcggca tcaatgattt cggctatctc
     1381 accccggacg acaaaggcca gcagcaattt gtctctctgg ttgaaaaact tccggaggat
     1441 gcccgccact ttgccgatgt ctggcgaacc tacagcacac caaacgatgt ggtctttcag
     1501 acggtgaatt atgtatttct ctggaacgga cagaccctgg acgttgtcca gccagcgcct
     1561 tccggaggca cattttctgc ctgtttgcag gttaatcaag acctgtatct tgtccaacgg
     1621 tcgctcggcc tcttcaaact ggtcaaccgg gcactccagc cagttcctgg cggtcaatgg
     1681 atggttgaaa aacggttatc gggattgctc ccgatgtcag atggaaaatt gctggccgtt
     1741 gtccgcgatg aaggactgtt tgtctatgat ggacaaacct ttacgccttt cgctgcccct
     1801 gaggtgtctc aagcactggt agcctgggat ccgtacaaag cgatggtcct gcctgatgga
     1861 cggtttgcgt ttgccaccat tacccatggc gcagtgattc tccggccaga tggcacgctt
     1921 gatgaaatta ttgataaaaa agccgggctc caaaaccagc aggtgaaaaa tattctggca
     1981 gatcgggatg gcaatctctg gatggggctg gataacggta tcgcccgggc agaagtcatt
     2041 tctccgttca cggtgtttga tgaccgctgc ggcatcgaag atgtggtcca ggcgttcatt
     2101 gaacacgccg gaaagtatta tctctcaacg gccaacggcg cctttgtctg ggatccgcaa
     2161 gttacccagc cctccggcgc accgcttttc cagtacattt caggccccaa aaccagttgc
     2221 tttgatttcg gaattgccgg aaatgatttg tttgtgtcgg cagataacgg cgtgtttcaa
     2281 ctcgaaggaa ccaaggcgac gaaagtcctg gattccctgg gaacagcggt ctccttttct
     2341 ttttgtacct cgaaacggta tccaaaccga ctctatgtcg ggcagcaata tggcctggca
     2401 gttctggaac gccggggttc aagctgggtc agtgctggaa aagttccagg tattgatacc
     2461 gaaatccgct ccattattga actttcagac ggaacactct ggcttggaac acatgcggag
     2521 gggatcctcc gagttgagtt tacatctctg tcagctccag aaagtttcaa tccggcaacg
     2581 gttcacgtca gtatccgacg ctttacggct caggatggac taccgatgat gtcgcggatt
     2641 cggctgatgc ggatttcaga tcggcttttg tttctgagtc aaaatggtat ggttgaattt
     2701 gacgaggcca ggggtcgctt tttcccatcc acttttctca gcgacccgac actcccacca
     2761 ctcagacgac tggtttccga ctcgcagggc acgttgtggg gatactttga agataatacc
     2821 tgtgcccggt taaccccagt tgctggtcgg tatcaggtgg atacgatcac gctgaagcgg
     2881 atgcctggag ccatttacaa ctgggtgttt gttgattcgc acaatactat ctggtttggt
     2941 gggagcaaag gtctcgttcg gattgaccca caacgcatca aacctccccc atcaccggtt
     3001 ccagttctga ttcgaaaagt tctggctgga aacaaacgcg acctgatgtt cggtggagca
     3061 accagcgcca caatgccagt gcttcatctt ccgtacacat ccaatcaggt gcggattgaa
     3121 tacgcactgc cgacttttat tcaagaacag tcaatccggt atcaatactg gctcgaaggc
     3181 gtagatgcaa actggtctga attgacaact gaaacaatca aggaatacac caatttacac
     3241 gaaggtcagt atcacattcg gattcgggca gaggatgccc ttgggtatca gagtttggaa
     3301 acagtgtacg cggttcagat tcttccgccc tggtatcgaa cctggtgggc ctatg
//
LOCUS       DAOQDO010000434         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21593_length_3353_cov_3.081868, whole genome shotgun sequence.
ACCESSION   DAOQDO010000434 DAOQDO010000000
VERSION     DAOQDO010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21593_length_3353_cov_3.081868"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..538)
                     /locus_tag="PKZ53_08230"
     CDS             complement(110..538)
                     /locus_tag="PKZ53_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HNJ40462.1"
                     /translation="MIRFLAFTLIAIFLFISGMHVFWAFGGRIGGMAAIPEINGKPTF
                     SPTAGMTLLVAFAFVCGALLVAGTSGVLQLPVPHSVLKWLTYLMAFIFLARSIGEFRL
                     VGFFKQIRDTQFAQMDTFFYSPLCLGIAIVAAIIGFKSPK"
     gene            complement(749..1252)
                     /locus_tag="PKZ53_08235"
     CDS             complement(749..1252)
                     /locus_tag="PKZ53_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40463.1"
                     /translation="MAQQIESIRSKVVIEANLFACFSEVRITVDITFHSDQLPWVVAT
                     QNRETIFDGQLPSERVTVFLNEVFQAAAKPERVTGERFTTHYEATLNWHNYPIPKLEA
                     SGQLHFKGHDLPEELLRENLHRLKGETAQRIKELLEVGLHCRPMVIFSIAHAFLENLR
                     DKSQSAD"
     gene            complement(1307..1837)
                     /locus_tag="PKZ53_08240"
     CDS             complement(1307..1837)
                     /locus_tag="PKZ53_08240"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNJ40464.1"
                     /translation="METGFDIKICPFCQETVKRSAKKCPHCQSWQNKAFGFQNHPVAT
                     IALMLIPFGMLFFINRQLFSNDKPQFADYQSQIQIMNAEMSFSTQNDCLTITTLGQIK
                     NQTDITWGDVSIEVQYFNAQGKLIDTGSDKTYDLVLQPRAEQAFRVRFCASKPQSEYA
                     SQKVFLRYAEKTPRWP"
     gene            complement(2064..2576)
                     /locus_tag="PKZ53_08245"
     CDS             complement(2064..2576)
                     /locus_tag="PKZ53_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNJ40465.1"
                     /translation="MGRIRYVAAMSLDGYIAGPNGEADWIIMDPEINFQALFAEFDTI
                     LVGRKTFEKMPKGRKSQKSPMPTFVFSQTLRPEDYPNVTIVSELSETSLSSIRAQAQK
                     DIWLFGGGGLFRSLLEVGQVDSIETAIIPVLLGGGIPLLPTPAKQTKLTLTEHKVYQT
                     GIVSLVYSTK"
     gene            complement(2934..>3353)
                     /locus_tag="PKZ53_08250"
     CDS             complement(2934..>3353)
                     /locus_tag="PKZ53_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40466.1"
                     /translation="PLPYSIKLAQGWSSHDEGMYVGYVPPTAPVGMDVYMMGTYSANP
                     PNATTLMEKVRSALAIQFARNFKPDVAPKDMKKVQIAGAEALYFEAPAPRPGTMWRQW
                     AFLKNGQAFVVVSIVAKDDKQVWTDVDAMVSSFQVAP"
BASE COUNT          897 a          789 c          783 g          884 t
ORIGIN      
        1 gaaccctgaa ccctgaaccc tgaaccctga accctgaacc ctgaaccctg aacccggaac
       61 cctgaacccg gaacccggaa cccggaaccc ggaacccgga acccggaact cactttggtg
      121 atttaaaccc aataatggct gccacaatgg caatgcccag gcagagcggg gaatagaaga
      181 acgtgtccat ctgggcaaac tgagtgtctc gaatctgttt gaagaaccca accagtcgga
      241 attcaccaat tgaccgggct aaaaagataa aggccatcag gtacgtgagc catttcagaa
      301 cactgtgtgg cacgggcaat tgaagaacgc cggaagtccc ggcaaccagc aatgctccgc
      361 acacaaatgc gaaagcgacc agcagggtca ttcccgccgt tggggagaat gttggctttc
      421 cattgatttc aggaatggcc gccatgcctc cgatgcgccc accaaaagcc caaaaaacgt
      481 gcatgccaga gataaacaga aaaattgcaa tcaatgtgaa ggcgagaaag cgaatcattg
      541 gattttaggg ttcagggtgt gaaaaacagg gttcagggtt cagggtttga aaaacagggt
      601 tcagggtagg gttatatgtt ttaggatcag ggttttaggc ccacagggtc atcgtgtaat
      661 agctgtggtg ttgccagccc acggtttttt catccatccc acaattcaag ccaggtagtg
      721 gagaatttca accaaatcaa aaaggagctt agtctgctga ttgagatttg tctctcagat
      781 tctccaaaaa ggcgtgggca atcgaaaata taaccatcgg acgacaatga agcccgactt
      841 cgaggagttc ttttatccgt tgtgcggttt caccttttag cctgtgtaga ttttctctga
      901 ggagttcttc gggaagatca tgacctttga aatgcagttg acctgacgct tcaagttttg
      961 gaattgggta gttatgccag ttgagagttg cttcgtagtg agttgtaaat cgttctcctg
     1021 taacccgttc tggtttagcg gctgcctgaa aaacttcgtt taggaaaacc gttacccttt
     1081 cggatggaag ttgcccatcg aagatcgttt ctctgttctg agtcgcaacg acccagggta
     1141 actggtctga gtgaaacgtg atgtcaactg ttatcctgac ttctgagaag caggcgaaaa
     1201 ggtttgcctc aatcacaact ttcgaccgaa ttgattcgat ttgttgcgcc atgactgcca
     1261 cgttgttttg gacttctctt cgctttgtct gatgaaaaga aactgattag ggccagcgag
     1321 gggttttctc agcataccgc aggaaaactt tttgactcgc atattcgctt tgaggttttg
     1381 aggcacagaa tctgacccga aacgcttgct cagctcgggg ttggagcacc aggtcgtagg
     1441 ttttatcact cccggtatca atcaattttc cttgagcatt aaaatactga acttcaatgc
     1501 tgacatctcc ccaggtgata tccgtttggt ttttgatttg accaagggtg gtgatggtta
     1561 agcagtcatt ttgagtcgaa aatgacattt ccgcattcat gatttgaatt tggctctggt
     1621 aatcggcgaa ttgaggtttg tcatttgaaa aaagctggcg attgataaag aagagcatcc
     1681 caaatggaat cagcatcaag gcaatcgtgg caactggatg gttctgaaat ccaaatgctt
     1741 tgttttgcca tgactggcaa tggggacact ttttcgctga ccgcttcacg gtttcctgac
     1801 aaaacggaca aattttaata tcaaatccgg tttccatagg tttgctcctt taagatgtgg
     1861 aacgattaaa ccaatcaggt cccctatcca ctcaggttcc caaaactcag agccataaca
     1921 gcctgacgaa ctcttgatgc accgataacc ttgaaaatga ataggatgtc aacagattgg
     1981 agggcctgac ccaaaatatt ctttgtccga caccattttt agacttaacc atcagcaata
     2041 tactcttcct caggctgaac tgtctacttt gttgaatata ccagcgagac aattccggtc
     2101 tggtacacct tatgttcggt caatgtcaac ttcgtttgtt tggccggagt tggaagtagg
     2161 gggattcctc cgccaagcag caccggaatg atggcggttt caatactgtc cacctgtcca
     2221 acctcaagca ggctgcgaaa tagccctcca ccaccaaaaa gccagatgtc cttctgtgcc
     2281 tgggcccgga tagatgaaag gctggtttct gacaattcgg aaacaatcgt cacatttgga
     2341 taatcttctg ggcggagcgt ctgtgaaaaa acaaaggtcg gcatcggaga cttttgggac
     2401 ttccttcctt ttggcatctt ctcgaaggtt ttgcggccta ccagaatcgt gtcgaactca
     2461 gcaaaaagcg cctgaaagtt gatctccggg tccatgatga tccagtcggc ttcaccattt
     2521 ggcccggcga tgtagccatc cagactcatc gcagcgacat atcggatgcg tcccataatt
     2581 tgaacctcat ttaaaaccca ctatctgagc ttcaacgcca aaaggccacc caaattgcgt
     2641 aaacaaacgt gcttataacc caggttgagg caaaccctct cattctttta ccgaaagtgc
     2701 cttcagtaaa atagcatcgt gcaagatgat tgaacaaatt tgacaaaccg acgaagagaa
     2761 ggagaccttt tcctgttttc acaagctcca ggaatgatat gggctgagtt aagtaataga
     2821 taagacaggc caaaattaga atcagattca taaccaacag gccatgcttt ttcatccagc
     2881 tatttgaagc tgattggtgc tccatgattg gctacccctt cattatcata gtgttatggt
     2941 gctacttgaa atgagctgac cattgcatcc acatctgtcc acacctgttt atcatctttg
     3001 gcaacgatgc tcacaaccac aaatgcctgc ccatttttca gaaaagccca ttgccgccac
     3061 atggttcctg gacgtggagc tggtgcttcg aaatacaggg cttccgcacc tgcaatttgg
     3121 acttttttca tgtctttggg agcaacgtca ggtttaaagt ttctggcaaa ttggattgcc
     3181 agcgcactcc ggactttttc catcaatgtg gttgcatttg gtgggttagc tgaatatgta
     3241 cccatcatat aaacgtccat tccaaccggt gcggtcggag gaacataacc gacatacatt
     3301 ccttcatcgt ggctcgacca gccctgagcc agtttgatgg aatagggaag tgg
//
LOCUS       DAOQDO010000435         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21599_length_3352_cov_3.569609, whole genome shotgun sequence.
ACCESSION   DAOQDO010000435 DAOQDO010000000
VERSION     DAOQDO010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21599_length_3352_cov_3.569609"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PKZ53_08255"
     CDS             complement(<1..1317)
                     /locus_tag="PKZ53_08255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNJ40467.1"
                     /translation="MAFPVLTNFPTFSDTLVIMPELMLTLFACFALVIDVLLPRERRG
                     LVAQFCLIGIGFSAVSLWMLYGELRPFSAQTAFYGTVVVDGFAVVFKSIFLLAALLSI
                     ILSMRYLDTEGMQRGEYYALILFATVGMMFMASGMDVIVLYVGLELMALSVYVLVGFL
                     KQLNPITSDDTTLNELQKHVNRSTEAGLKYFLMGAFSSGVLLYGLSLLFGVTGTTYLP
                     DMGEALFQYLTPNARTGNPQMLILLGIILTGAGLLFKIAAVPFHMWAPDAYQGAPTSI
                     TAFMSVGPKAASYALLARIFLVGFEPFRTINQLPGWAILLGVIAGLTMTVGNIGALTQ
                     SNVKRLLAYSSISHAGYILLGIVAGNEFGYTGVVIYLLVYVLMNLGAFGVLLVLRRQD
                     LAGEKLDDFNGLFHKAPGLAAMMLIFLLSLAGIPMTGGFIGKFYLFG"
     gene            complement(1481..3277)
                     /locus_tag="PKZ53_08260"
     CDS             complement(1481..3277)
                     /locus_tag="PKZ53_08260"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNJ40468.1"
                     /translation="MLAALFLSGFQPAELKPFGIGLLSLVLWMPVLGALILIFGINKD
                     KANLIKYFTTAWMGVSFVLMFPLLALWKHEVPGLHFIEDSSWIPMIGARYQLGIDGLA
                     LVLVLLTGFLGVIAALCSWNYIEIRLKEYYAFLLLLQTGMIGVFVAADTFLFYVFFEV
                     MLVPMYFLIGIWGGERRVYAAIKFFLYTLFGSVLLLLAILKMYFLAPEIARAHPAEVK
                     AAAVQLAGDNRPMLQMIEAGIETAKQAGIRSDSPAGTLNIYAMQAIGSARDVRGLSFI
                     PLIVQLWVFGGFAIGFMIKVPMFPFHTWLPDAHVEAPTAGSVVLAAVLLKMGTYGFLR
                     FNIPMLPDAAVHPLTLSVISTLAIIGIIYGSLVAMAQTDMKKLVAYSSVAHMGLCMLS
                     MFALNPNGINGSILQMLNHGISTGALFLLVGVIYERRHSRQIAEYGGLAGVTPQYAVI
                     FLITTMSSIGLPMLNGFIGEFVGLRGIFEANLKWAVFGVLGIVLGAAYMLWLYQRVFF
                     GAVTNPKNQTLPDLSIREWVYLTPLVVLMFWIGLYPKPVMDLIDGPTDTIVRQVKPGY
                     FKPVPPALATPKTADSAGAPHPPEIKHDVPVK"
BASE COUNT          976 a          894 c          807 g          675 t
ORIGIN      
        1 gccaaagaga tagaatttcc caataaaccc gcctgtcatt ggaatccccg ccagactgag
       61 cagaaaaatc agcatcatcg ccgccagacc tggggctttg tggaagagtc cattaaagtc
      121 atccagtttt tctccggcca gatcctggcg tctcagtacc agcaacacac caaaggcacc
      181 gagattcatc aacacataga ccagcaggta gatgaccacg ccggtgtatc caaattcatt
      241 cccggcaaca attccgagca aaatataccc ggcatgtgaa atgctcgaat aggccagcag
      301 tcgcttgacg ttggattggg tgagtgcccc aatattgcca actgtcatcg tcagtccggc
      361 gataaccccc agcaggattg cccatcctgg caactggtta attgtccgga aaggttcaaa
      421 tccaaccaga aaaatccggg ccagcagcgc ataactggcg gcttttggac ccaccgacat
      481 aaaggccgta attgacgttg gtgctccttg ataggcatct ggggcccaca tatgaaacgg
      541 aaccgctgca attttgaaga gcagtccagc cccagtcagg ataatcccca gcagaatcaa
      601 catttgcgga tttccagtac gtgcattggg ggtcaggtac tggaacagcg cctctcccat
      661 atccggcaaa taggttgttc ccgtgacgcc aaacagcagt gacaacccat agagcaacac
      721 cccggacgaa aacgccccca tcaggaagta tttcaagccc gcttcggttg agcgattgac
      781 gtgtttttgg agttcattca gggtcgtgtc atcactggta atcggattga gttgtttgag
      841 aaagccaacc aggacataga ctgaaagcgc catcaattcc agaccaacat agagcacaat
      901 cacgtccatc cccgatgcca taaacatcat gccaaccgtc gcaaacaaga tcagggcata
      961 gtattcgccg cgctgcattc cttcggtatc aaggtatctc attgataaaa tgatagatag
     1021 cagtgcagcc agcagaaaaa tactcttaaa gacgaccgca aatccatcca ccacaaccgt
     1081 accataaaag gcggtctggg ctgaaaacgg gcgcagttcg ccatagagca tccacaacga
     1141 cacggctgaa aatccgattc cgatcaggca gaactgtgcc accagccccc gtcgctcgcg
     1201 tggaagcagg acatcaatta ccaatgcaaa acaggcaaag agcgtcagca tcaactctgg
     1261 catgatcacc agcgtgtctg aaaatgtggg aaaattggtc agcacaggaa aggccataag
     1321 tttcggagtc caacctcagc gttcagtgtt caatcttttg cttgcaaaac ttttccaaca
     1381 ggtcattcaa gttgttgatg aaaccagggt taacctcatc gtaagctgga aggcaagcga
     1441 aaacttgaac tcggtacttg attctaaaga gcaatcgtca ttacttcaca ggcacatcgt
     1501 gcttgatctc tggtggatgc ggtgcaccgg ctgagtctgc tgtttttggc gttgccagag
     1561 ccggaggtac cggtttgaaa tagcccggct tgacctgtcg cacaatggta tctgttgggc
     1621 catcaatgag atccatcact ggtttgggat agagcccaat ccaaaacatc aacaccacca
     1681 gcggtgtcaa atagacccat tcgcggatgc tcaggtcagg cagcgtctgg tttttcggat
     1741 tggtaaccgc accgaaaaac acccgttgat acagccacaa catataggct gcccccagaa
     1801 cgatgcccag cacaccaaag acagcccact tcaaattggc ttcaaaaatg cctcgcaggc
     1861 caacaaattc accgataaat ccattgagca ttggcaggcc gattgatgac atggtggtga
     1921 tgagaaaaat gacggcatat tgcggtgtca ccccagccag ccctccatat tcagcaattt
     1981 gccggctatg gcggcgctcg taaatgaccc caaccagcag gaagagcgcc ccggttgaaa
     2041 tcccgtgatt gagcatttgc aagatcgaac cattaattcc attggggttg agtgcaaaca
     2101 tcgagagcat gcacaacccc atgtgggcca ccgatgaata cgccaccagc tttttcatgt
     2161 cggtttgcgc cattgccacc agcgaaccat aaataatgcc gataattgcc agtgttgaaa
     2221 ttacagacag ggtgagggga tggaccgctg catcggggag cattggaata ttaaatcgca
     2281 gaaacccata ggtccccatt ttgagcagca cggcggccag caccaccgac ccggcagttg
     2341 gtgcttcgac gtgggcatct ggaagccagg tgtgaaaagg gaacatcggg accttgatca
     2401 taaaaccgat ggcaaatccg ccaaaaaccc acaactgcac aatgagcgga ataaatgaaa
     2461 gccctcgaac atcacgggcg ctcccaatgg cctgcatggc gtaaatattg agcgtcccag
     2521 ccggtgaatc agaccggata ccggcctgtt tggcggtttc aatcccggct tcgatcatct
     2581 gcagcatcgg acgattatcg ccagccagtt gcacggcggc ggctttgact tcggctggat
     2641 gcgcccgggc aatttccggg gccagaaaat acatttttag aattgccagc aggagcaaca
     2701 ccgacccgaa cagcgtgtac aaaaagaact taatcgctgc atacacccgc cgttcgccac
     2761 cccaaatgcc gatcagaaag tacatcggca ccagcatgac ttcaaaaaag acataaaaca
     2821 gaaaggtatc tgccgcaaca aacacgccga tcattccggt ctggagtaac agcaaaaacg
     2881 cataatattc cttgagccga atttcaatgt agttccagga acacagcgcc gcgatgacac
     2941 cgagaaaccc ggtgagcaac accagcacca gcgccaatcc gtcaattccc agttgatagc
     3001 gggcaccaat cattggaatc cagctagagt cttcgatgaa atgcagccct ggtacctcgt
     3061 gcttccataa agcaaggagt ggaaacatca gcacaaaaga cacacccatc caggcagtgg
     3121 tgaagtactt gatcaaattc gctttgtctt tattgatgcc aaaaatcagg atgagcgctc
     3181 cgagcaccgg catccagagc accagtgaca gcagcccaat gccgaatggt ttgagttcgg
     3241 ctggctgaaa tccagagaga aacaaggcag cgagcatgac agactcctga aagaacggaa
     3301 tgaagaattg agaatgaaga atgaagaatt gagaattgag aattgagaat ga
//
LOCUS       DAOQDO010000436         3344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21689_length_3344_cov_3.318334, whole genome shotgun sequence.
ACCESSION   DAOQDO010000436 DAOQDO010000000
VERSION     DAOQDO010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_3344_cov_3.318334"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..786
                     /locus_tag="PKZ53_08265"
     CDS             67..786
                     /locus_tag="PKZ53_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40469.1"
                     /translation="MMSIHRRIHVSLLWMWLLAIPVFGQQPSLARPLPPLPANSRLVD
                     QLVASVNGEIITQSDLVWLIGLDPKAIVTNIEEDRLREALSRKINQVLLDQEARQLPN
                     VVITQPDIRQYERQLVTLFGSEVLFRQRLEAIGLTSEMLSQIVRAQIEIEKYTDFRFR
                     SFVLVTEEEIKSAYRAEREQSKSSGTVLPEDPSDETYDRLQDQLLERKVREEIDNWLK
                     TARARAEIVPLAPFAQSVIGQ"
     gene            1147..3258
                     /gene="nrdD"
                     /locus_tag="PKZ53_08270"
     CDS             1147..3258
                     /gene="nrdD"
                     /locus_tag="PKZ53_08270"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNJ40470.1"
                     /translation="MVDETLSPVSFTSTAAASGPTEYSPELDLLVRHSDERIVRFDRR
                     RIVTTLIRETGLKPELADKISYEIQAMLSRAGVTKVSSSLIQSLVDARLIEHGLEREF
                     VAHSRLGIPRYELDQIIHQSARAEHTQPLGPEGTSLRLAEAIKREYAILSVFSEPVAK
                     AHLIGDIYIQNLGGVDRPRLLVQSLDSIKRYGITLPQSFANWRPARHPEVLVAHLVKF
                     SAALQGYINGPIEWDAVNYCLAPYVEDLDAKSLKQLAQGLLFELSSPSAARGGQMLTC
                     TLHLDWEVPPYMGDRIAIGPGGESTGRSYRDYSEAAQKLLRAIYEAYLEGDGQKLPFT
                     GAQLVLHIAQHFSESVGYKGLLDTVSQVATGRGGIHIVFDRNNAHSFYRRYGVPLEGA
                     LSRADTTEWCTAVFQAVGVNLPRAAYRSGSTNQTAILEELTRLMDVAAQAHLEKRIFL
                     EKLMALGEQGPLALLAMRRQGNTFLRLGRTTHWLCPIGLNELVWAVTGQQLHESQEAR
                     EFGKRVIAHIAAETVRLSSRHKTRFLLAECSAEEAPHRLAQLDLQFFPDESAEFVQGQ
                     VPTGDVFYSNGVKLNSKIPVSALERAQLEGFLHNDQLINTTSEIWLGSQPVAASTVAV
                     FISQAFYRSQASGLVFAPEFTACLACGAVSHGLQTRCTTCGSAHIDGIAQAGLSYSRT
                     SNMTDSQMIELKNRYRESLKD"
BASE COUNT          806 a          911 c          840 g          787 t
ORIGIN      
        1 tgtcaagcca ccgcactcca caaaccttaa agcggtgtca agccaccgca ctccaaggct
       61 ttatatatga tgtccattca ccgccgaatc cacgtgagtt tgctctggat gtggttgctt
      121 gcaatccccg tgtttgggca gcaaccctca ctggccaggc cgctcccgcc actgccagcc
      181 aatagccggc tggttgacca gttggtggca tccgtcaacg gtgaaatcat tacccaaagc
      241 gatctggttt ggctgattgg actcgacccc aaagccatcg ttaccaatat tgaagaagac
      301 cgcctgcgcg aagccctttc ccgcaaaatc aatcaggtct tgcttgacca ggaagcccgc
      361 cagcttccaa atgtcgttat cacgcaaccg gatatccgcc aatatgaacg gcaactggta
      421 acactgtttg gctcggaagt tctttttcgg caacgacttg aggctatcgg attgacttcg
      481 gaaatgctct cgcaaattgt ccgggcccaa attgagattg agaaatacac ggactttcgg
      541 tttcggtcgt ttgtccttgt caccgaagaa gaaatcaagt cggcatacag agctgaacgc
      601 gaacaatcaa aatcaagcgg aacggtactc cccgaagacc cgtcagacga gacctatgac
      661 cgcctgcaag accagctcct ggaacgtaaa gtccgcgaag aaattgacaa ctggttaaaa
      721 acagcccgcg cccgggctga gattgtcccg ctggccccat ttgcccagtc agttatagga
      781 caataggaag tacggcctaa aatcctcatc ctggcgctta tgggtatcac cccacgcccg
      841 ctcgcgaaat tcaggcagta agctgaagga acctgtggta tgattgcact tcagtttatc
      901 cagtatcccc aattaaaaaa tcagtttggt tcgattgtag tctggtgact gatatttgga
      961 actggaacga caccttttca aaaaccagat atcaggtatc aatcatcacc caaacgcgtc
     1021 tttcataagc ctcaaatgta gagtactcca ggcagattgc ctgacaaatc agctgggcaa
     1081 gtttcgtttt cctcacgcct tgatgcttgc agggaaccaa ctcgctcatc ggcacgggat
     1141 tacgctatgg tggacgaaac cttgagccct gtatcattta ccagtactgc cgcagcatct
     1201 ggacccactg aatactcccc cgaattagac ctcctggtac gtcattcaga cgaacgcatt
     1261 gtccgatttg atcgccgccg gattgtgacc accctgattc gggaaaccgg cctcaaaccc
     1321 gaactggctg acaaaatcag ttatgaaatc caggccatgc tgagccgtgc cggcgtgacc
     1381 aaagtcagtt cgtccctgat tcaaagcctg gtggatgcca ggctgattga acatggactg
     1441 gaacgtgagt ttgtcgcgca ttcacggttg gggattcccc gttatgaact agatcagatt
     1501 attcatcaat cagcgcgggc cgaacatacc caaccactcg gccctgaagg taccagcctg
     1561 cggctggccg aagcaatcaa acgcgagtat gccatcttgt cggttttttc tgaaccagtg
     1621 gccaaagctc atttgattgg tgatatttac attcagaatt taggcggagt tgatcgcccg
     1681 cgattactgg ttcaatcgct ggattcaatc aaacgttatg gcattaccct gccccagtca
     1741 tttgccaact ggcgcccggc acgtcaccca gaggtcctgg tggcgcacct ggtgaagttt
     1801 tccgctgcgc tccagggcta catcaacggc cccatcgagt gggatgccgt caattattgt
     1861 ttagcaccgt atgttgaaga tctcgacgcc aagtcactga aacaactggc ccaggggttg
     1921 ctctttgaac tctcttcacc gtcggcggct cgcggcgggc agatgctcac ctgtacactc
     1981 catcttgact gggaagtccc gccctacatg ggtgaccgga ttgcgattgg ccctggcgga
     2041 gaaagtacgg ggcgttcgta ccgggactat tccgaagcag cccaaaagct cttgcgcgct
     2101 atttatgaag cgtacctgga aggcgatggc cagaaattgc cattcaccgg ggcacaactg
     2161 gtcctgcata ttgcccagca tttctcagaa agtgttggat ataaagggtt acttgataca
     2221 gtcagtcagg tggcaaccgg tcgaggcggg attcatatcg tctttgatcg aaacaatgcc
     2281 cacagttttt accgccgcta tggtgtaccg ctcgaagggg cactcagtcg tgctgacacc
     2341 actgaatggt gcaccgcagt ctttcaggcg gtcggagtca accttcctcg cgcagcctat
     2401 cgctctggat ccaccaacca gactgccatt ttggaagaat tgacccggct gatggacgtg
     2461 gcggcccagg cccatcttga aaaacggatt tttcttgaga aactgatggc gcttggtgag
     2521 cagggtccac ttgcgctcct ggccatgcgc cgccagggaa atacatttct tcggctgggt
     2581 cgaacgacac actggctttg cccaatcgga ttaaatgaac tggtgtgggc agtcaccggg
     2641 caacaactcc acgaatcaca ggaggcccgc gagtttggaa aacgtgttat tgcccatatt
     2701 gccgctgaaa ccgtgcgcct gtcgtcacgg cataaaacgc gttttctgct ggccgaatgt
     2761 tctgccgaag aagctcccca ccgtctggcc cagcttgacc tgcaattttt cccagatgaa
     2821 tcggcggaat ttgtccaggg tcaggttcca actggcgacg tgttttattc gaacggggta
     2881 aaactgaatt ccaaaatacc agtttcagcc ctcgaaagag cccaacttga gggcttttta
     2941 cacaatgacc agttgatcaa caccaccagc gaaatctggc ttgggagcca accagtggcc
     3001 gccagtaccg tcgcggtttt tatttcacag gcattttacc gatcccaggc cagcggtctg
     3061 gtctttgccc cggaatttac tgcctgtctg gcctgtggcg cggtaagcca tgggttgcaa
     3121 accagatgca ccacgtgcgg ttcagcacac attgatggaa ttgcccaggc tggactttcc
     3181 tacagtcgaa cgtcaaatat gactgacagc cagatgatcg agttgaagaa tcggtaccgg
     3241 gaaagcctga aggattaaat ctttttgagt cataagtcag tcgtcagtcg tcagtcgtca
     3301 gtcgataaaa cccggatatt gtagtgtgtg gagttcttga aaag
//
LOCUS       DAOQDO010000437         3344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21692_length_3344_cov_3.117057, whole genome shotgun sequence.
ACCESSION   DAOQDO010000437 DAOQDO010000000
VERSION     DAOQDO010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21692_length_3344_cov_3.117057"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..>3344)
                     /locus_tag="PKZ53_08275"
     CDS             complement(222..>3344)
                     /locus_tag="PKZ53_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40471.1"
                     /translation="FLGTNDQPGDYRSSGCTACHTPYANDRFEAHSAQFAKYGHEGKS
                     FTADPTIPKNEPGHPIKHQLTSAIPTSQCLVCHMHPGTNLVTTYAGFMWWDNETDGDK
                     MYPEKSAKLSNEELENIRIRNPEGSAQKGKWGTDTEFLNQTGSPDFNKQLKRTQFADF
                     HGHGWMFRAIFKQDRKGHFLDAQGNKVENVTGETMHQATQPYTEAEYKAWQENKPVRP
                     GVPVHLKDIHLDAGMHCVDCHFSQDGHGNGKLYGEPRAAIEISCIDCHGSIYKKALSD
                     GKNTVTTGFAGGNKLSKYKVWVKGKSKKQPVLELKDGKIIQHSALDPEKEWEVVQTVD
                     TIDPASPHYSEKSRLAKTMQKDGRSWGAVPNQAPTYKWDPDKKQTVLANNPDFELAHN
                     DNRMTCYSCHSSWMTSCFGCHLPMVANQKRNMAHNEGGPETRNWTNYNYQVLRDDVFM
                     LGVGGTATGAVESGRDSGGMIAPVRSSSAVIVGSKNANRNWLYSQQQTISAEGYSGQA
                     MNTHVPHTVRGKGETKQCTDCHISKENDNNAIMAQLLLLGTNYVNFMGRYIYVAAGEH
                     GFEAVVATERDEPQAVIGSSLHKLAYPEEYKKHVDNKRELTEAYEHHGHVVSLQMRGE
                     YLYAAEGEKGIHVYDIANIDDKDFSERITSAPVSPLGQKFYVKTKHATAIASPTTLGV
                     DPTREHLPENEEQPISLVYAFLYASDKEEGLVVIGDGLSGKHGPGVATLLDGDPANNF
                     LARQPGFNPDGKLSGAENLTVAGNWVYLLCDKGVAVIDISNPLKPELKTIVEGFHEPK
                     SIAVQFRYAFVTDEEGMKVIDITRPERAKLVKDAEVPIKHAHGIYVARTYAYIAAGEE
                     GLVIVDVEKPTEPKLDQTYNANGEINDAHDVKVGMTNASAFAYIADGKNGMRVLQIMS
                     PETNPNFLGFSPRPMPELIATYPMKGHALAISKGLDRDRGVDESGNQLVVFGRRGARP
                     FNLEEMRTMMRPRVTNEPPGPPINAAPPTMGLLTNISEYFKNLDAANALFGLALVLLP
                     WPFLRRRKDE"
BASE COUNT          706 a          870 c          863 g          905 t
ORIGIN      
        1 ggttgcaaca accctggtga gcaggattcc agagatcacc agggagaaaa caacgagagc
       61 catcaaacga cgcgagttct gcacaaccac ctcttttcgg caacgactgt tgagagttcc
      121 gccttcaggc ggccctatcc ttacaatata tatcgtcaat attcttcaag acattgctgt
      181 ctgaaagcga aactctgaac ttttcctaaa aagtatgagc actactcgtc tttccgacgt
      241 ctgaggaatg gccacggcaa cagtaccaga gccagaccga agagtgcgtt ggccgcatcc
      301 aggttcttga aatactcaga tatgttggtg agcaatccca tggtcggcgg agcggcattg
      361 atgggcggtc ctgggggttc gttcgttacc cgtggccgca tcatcgtccg catttcctca
      421 agattaaatg gacgtgcccc acgacgacca aacacgacca gttgatttcc gctttcatcc
      481 acaccccggt ctcgatccaa tccctttgaa atggccagcg cgtggccttt cattggatag
      541 gtcgcaatca attccggcat tggccgcggg ctaaagccca ggaagttcgg attggtttca
      601 ggtgacataa tttgcagcac ccgcattccg ttctttccat cggcgatgta ggcaaaagcg
      661 ctggcattgg tcatgccgac tttcacgtcg tgagcatcat tgatttcacc gttggcgttg
      721 taggtctggt caagttttgg ttcggttggt ttttcaacgt caacaataac cagaccttct
      781 tcaccagccg cgatataggc ataggtccga gccacgtaaa taccgtgggc atgtttaatc
      841 gggacttcgg cgtctttgac cagtttggcc cgttcgggtc gggtaatgtc aatgactttc
      901 atcccttctt cgtcggtgac gaaggcatat cggaactgaa cggcaatgct ttttggctcg
      961 tggaagcctt cgacaattgt cttcaattca ggtttgagcg gatttgaaat atcaatgact
     1021 gccacgccct tgtcacacag gaggtacacc cagttgccgg ccacagtcag gttttcagcc
     1081 ccgctgagtt tgccatctgg gttaaaccct ggttgacgcg ccaggaagtt attggccggg
     1141 tcgccatcga gcagggttgc cacgcctgga ccatgttttc cgctcagacc atccccaatc
     1201 accaccagtc cttcttcctt atcggaagca tacaggaaag cgtagaccag actgattggc
     1261 tgttcttcgt tttcaggcag gtgctcgcgg gtggggtcaa ccccaagcgt ggtcggagaa
     1321 gcaatcgccg tggcgtgttt ggttttgaca tagaacttct gccccagggg cgagacggga
     1381 gcggatgtaa tccgttccga gaagtctttg tcatcaatgt tagcaatgtc atacacgtgg
     1441 atgccttttt cgccttcagc cgcatagagg tattcacctc gcatttgcag gctgacgacg
     1501 tggccgtggt gttcataggc ttcagtgagt tcacgtttgt tgtcaacgtg tttcttgtac
     1561 tcttctggat aggccagctt gtgcaacgaa ctgccaatca cggcctgggg ttcgtcacgc
     1621 tcagtagcca ccacagcttc aaagccatgc tcaccagccg ccacataaat gtagcgaccc
     1681 atgaagttga catagttggt ccctaacaac aacaactggg ccataatggc gttgttgtca
     1741 ttttctttcg aaatatggca atccgtacat tgcttggttt caccctttcc acgcacggtg
     1801 tgtggaacgt gggtgttcat cgcttgaccg gaataccctt cagccgaaat ggtttgctgt
     1861 tgcgaataga gccagttgcg gttggcgttc tttgaaccca cgatgaccgc acttgatgat
     1921 cgcaccggag caatcatgcc gccagaatcg cgtccagatt caaccgcacc cgtcgctgtt
     1981 ccgccgacgc cgagcataaa cacgtcatca cgcagaacct gatagttata gttggtccag
     2041 ttacgagttt ccggaccacc ttcgttatgg gccatattgc gtttctgatt ggccaccatt
     2101 ggcaggtgac agccaaagca actggtcatc cacgacgagt gacaggagta acaggtcatc
     2161 cggttatcgt tatgggccag ttcaaagtcc gggttgttgg ccaacacggt ttgcttctta
     2221 tctggatccc acttgtaggt aggtgcctgg tttggcacgg ccccccacga acgaccatcc
     2281 ttttgcatgg ttttggccaa tcgggatttt tcgctgtagt gcgggctggc cgggtcaatc
     2341 gtatcaacgg tttgtacgac ttcccattct ttttccggat caagggcgga atgttgaatg
     2401 atctttccat ccttgagttc taaaaccggt tgtttcttgc tcttgccttt gacccagact
     2461 ttgtatttgg aaagcttgtt tccgccggca aaaccagtgg ttactgtgtt tttaccatcg
     2521 gagagtgctt tcttgtagat tgatccgtgg caatcaatac aggaaatttc aatcgcggcc
     2581 cgcggctcgc catacagttt tccattgccg tgtccatctt gtgagaagtg acagtcaacg
     2641 cagtgcattc cggcatcaag gtggatatcc ttcaaatgga ctggaacccc tggccggaca
     2701 ggtttatttt cctgccaggc tttgtattcc gcttccgtgt atggttgcgt ggcctgatgc
     2761 atggtttcgc cggtcacatt ctcaacttta tttccctggg catccaggaa atggcctttg
     2821 cggtcctgtt tgaaaattgc ccggaacatc cagccatggc cgtggaagtc agcaaactga
     2881 gtgcgcttga gttgtttatt gaaatccggt gagccggttt gattcaaaaa ttcggtatcc
     2941 gttccccatt tgcccttctg ggctgatcct tcagggttgc ggatgcgaat attttccagc
     3001 tcttcattgc tgagtttggc gctcttttcc ggatacatct tgtcgccatc ggtttcattg
     3061 tcccaccaca taaacccggc ataggtggtc accaggtttg tgccagggtg catatgacaa
     3121 accagacact gactggtcgg gatggcgctc gtcaactggt gtttaatcgg gtgacccggt
     3181 tcattttttg gaatcgtcgg gtcagccgtg aacgatttgc cttcgtgccc atatttggca
     3241 aactgggcag agtgcgcctc aaaccggtcg ttggcatacg gggtatgaca ggcagtacaa
     3301 ccgctggagc ggtaatcccc tggttgatcg ttggtgccca ggaa
//
LOCUS       DAOQDO010000438         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21707_length_3343_cov_2.718674, whole genome shotgun sequence.
ACCESSION   DAOQDO010000438 DAOQDO010000000
VERSION     DAOQDO010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21707_length_3343_cov_2.718674"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PKZ53_08280"
     CDS             <1..719
                     /locus_tag="PKZ53_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HNJ40472.1"
                     /translation="EHIVCVHGGSDETSRLAEKLEHPPRFVTSVSGHTSRLTDRQTLE
                     IFLMATSLINRKLVEAFQSRGINAIGLSGLDGRLITARRKEAIRILDQGRQRVIRDDW
                     TGTPEAVNTSLLSMLLNSGYLPVIAPVAISSVGEAVNVDADRLAALVAGALNAQTLVL
                     LTNVPGVLRSFPDESTLIRRLSRSEPGASDQFVTGRMRKKLLGASEALSSGVETVVIG
                     DARRLHPLSAALAGEGTVIQ"
     gene            716..1891
                     /locus_tag="PKZ53_08285"
     CDS             716..1891
                     /locus_tag="PKZ53_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNJ40473.1"
                     /translation="MSAHTIVDPAQVEARHSLGIFGPRGLTLVRGAGARVWDDQGKSY
                     IDATGGYGVASVGHSHPTLVSAVTAQAQRLISCPAFFTNDQRSMYLTRLASFLPPDLN
                     RIFLCNSGTEAIEAALKFARFTTGRTGVVAAMRGFHGRTFGALSATWESHYREPFAPL
                     LAGFEHVPLNNAAALDGTITDKVGAVLLEVVQGEGGVRPALAEFLQTARTLCLERGAM
                     LILDEVQTGFGRTGQMVACSHVDIVPDLLCLAKGIAGGIPMGAVAVGPRVKQLTVGAH
                     GSTFGGNPLACAAASAVLDIFENEQLVDQSAIKGTYLKDRLRQIRSPKIREVRGLGLM
                     LGIELRERATPYLKAMQSAGVLALNAGPNVIRLLPPLVIETSELDTIADVIEAVLSV"
     gene            2063..3133
                     /locus_tag="PKZ53_08290"
     CDS             2063..3133
                     /locus_tag="PKZ53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HNJ40474.1"
                     /translation="MNDAAALNLLEAMVRIPSYSGQETALAAFLVNEMNQAGLVSQID
                     DAGNAVGIVETGNCDESSPTVVLLGHMDTVQGWIPIRVEDGKLYGRGAVDAKGPLAAF
                     IVAASRFQKLCSSPARLVVIGCVEEEAPSSKGAHFVVPHYRPTYCIVGEPSRWDRVTL
                     GYKGFLRVTARLKTDVTHSAHPTPTAAEVGCASWHRVAAAAERFNVHRERLFDQLLPT
                     LVGIQSTSNGCHEFVVLRINLRLPPDLPPEAAAVWLAEQLPGFELEPVGGLPAWSGPR
                     TTALHRAFGRAIRAQGRDVQFQLKTGTADMNIVAPAWGCPTVAYGPGDAALDHTPHEH
                     IELDEYLKSISVLEQVLRSAVA"
     gene            complement(3141..>3343)
                     /locus_tag="PKZ53_08295"
     CDS             complement(3141..>3343)
                     /locus_tag="PKZ53_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40475.1"
                     /translation="ALAAILPNGAPDAHVAQEVDLQVQYNFRPGVSFGSGFGHWIPGA
                     FWKAATPGSPQTFAYSFMTYRF"
BASE COUNT          728 a          891 c          950 g          774 t
ORIGIN      
        1 gagaacatat cgtttgtgtc cacggcggat cagatgaaac cagccgtctg gctgaaaagc
       61 tggaacatcc gcccaggttt gtgacttccg tgtctggcca taccagtcgg ttgacggatc
      121 gtcagacgct tgaaatcttt ctcatggcga ccagcctcat caatcgaaaa ctggtcgaag
      181 cctttcaatc acgaggtatc aatgcaattg ggctttccgg attggacgga agattaatca
      241 ccgctcgtcg caaagaggcc atccgcatcc ttgaccaggg ccgacagcgg gttatccggg
      301 atgattggac gggcacccct gaagcggtca acaccagttt gctctcgatg ttgctgaatt
      361 ctggatactt accggtgatt gcgccagttg cgatctcatc tgtcggagaa gccgtaaacg
      421 ttgatgccga ccggctggca gcactggtgg caggggcact caacgcccaa accctggtgc
      481 ttctgaccaa tgttccgggt gttttgcggt cttttccgga tgaatccacc ctgattcgcc
      541 gactttcaag aagtgaacct ggcgcgtcag atcagtttgt gacaggccgc atgcgaaaaa
      601 aactgctcgg tgcctctgaa gcactttcct ccggggttga aaccgtggtg attggtgatg
      661 cccgacgatt acatccgctc agtgccgccc tggctgggga agggacggtc atccaatgag
      721 tgctcacacc atagttgacc cagctcaggt cgaagcccgg cacagtttag gaatctttgg
      781 ccctcgtggt ctcaccctgg ttcgcggtgc aggtgccaga gtttgggacg atcagggaaa
      841 aagctacatt gacgccacgg gcggatacgg cgtcgccagc gtagggcatt cgcacccaac
      901 gcttgtttca gccgtgaccg cccaggctca acggttgatc agttgcccgg cttttttcac
      961 caacgaccag cgttcgatgt acctgacccg tctggccagt tttctcccgc ctgacctcaa
     1021 tcggattttt ctatgtaatt ccggcaccga agccatcgaa gccgcgctca aatttgcccg
     1081 gttcacaact ggtcgaacgg gtgttgtcgc tgccatgcgc ggattccacg gacggacctt
     1141 tggggcgctc agtgccacct gggaatctca ctatcgtgaa ccattcgcgc cgcttctggc
     1201 tggattcgag cacgttccgt tgaataatgc agcggccctg gacggcacca taaccgataa
     1261 ggttggtgcc gtgctgctcg aagtcgttca gggcgaaggc ggcgttcgac ccgcgctggc
     1321 tgaatttctc caaacggctc gaaccttatg tttggaacgc ggggcaatgc tcattctgga
     1381 tgaagtccaa accggttttg gtcgaacggg tcaaatggtt gcctgctcac acgttgacat
     1441 cgttccggat ctgctctgtc tggccaaagg tattgccgga ggcattccaa tgggtgcggt
     1501 ggcggttggt ccacgagtaa aacaactcac cgttggcgcg catggttcca cctttggtgg
     1561 caatccgctg gcctgtgccg cagccagtgc agtcctggat atttttgaaa atgaacagct
     1621 tgtggaccaa tctgccatca agggaacgta cctgaaagac cggttgcggc agattcggag
     1681 tcccaaaatc agggaagtgc gtggactcgg gctgatgctt ggaattgagc ttcgcgaacg
     1741 ggcaacgcca tatttaaagg ccatgcagtc agcgggcgtt ctggcgctca atgccgggcc
     1801 aaacgtgatt cggctgttac ctccgctggt gatcgaaact tcggaactcg acaccattgc
     1861 cgatgtgatc gaagctgttt tatcagtttg aaggtaagtc ttttacaaaa acccagaaga
     1921 catctttaac cacgaaatac acgaaataca cgaaaaaaac acttataaac atcaatctcc
     1981 cagtagttta ctgattggat atttaattgg gttctttttc gtgtgtttcg tgtgtttcgt
     2041 ggtttaatga gaggaagtag ttatgaacga cgctgctgca ctcaacttgc ttgaagcgat
     2101 ggtccgcatt ccaagctatt ccggccagga aacggcgctg gcggcatttc tggtgaacga
     2161 aatgaatcag gccggattgg tcagtcagat tgatgacgcc ggaaacgcgg ttggcatagt
     2221 cgaaaccgga aattgtgacg aatcaagccc caccgtggtg ttgcttggcc atatggacac
     2281 ggttcaaggt tggattccga ttcgggttga agatggaaaa ctctatggac gcggcgcggt
     2341 tgatgccaaa ggaccgctgg cggcgttcat cgtggcggcc agccgattcc aaaaattgtg
     2401 ttcctctccg gccagactgg tggtgattgg ttgtgtcgaa gaagaagccc cgtcgtccaa
     2461 aggtgcccat tttgtcgtgc cacactaccg ccccacgtat tgcattgttg gtgaaccgag
     2521 tcgttgggat cgggtaacgc ttggatacaa aggctttttg cgggtcacag cccgactgaa
     2581 aactgatgtc acccactcgg cgcatccaac accaacagcg gctgaggtgg ggtgtgccag
     2641 ttggcaccgt gttgctgcgg ctgccgaacg gttcaacgtg catcgcgaac ggctctttga
     2701 ccagttgctg ccgacgctgg tgggcatcca gagtacttcg aatgggtgtc atgaatttgt
     2761 ggtactgcgc atcaacctgc gacttccgcc ggatttgcca cctgaagccg ctgccgtgtg
     2821 gctggccgaa cagttacctg gatttgaact tgaaccagtt ggggggcttc cagcctggag
     2881 cggtccgcgt accacagcac tccatcgagc ctttgggcga gcgattcgtg cccaggggcg
     2941 agatgttcaa ttccagctta aaaccggcac ggcagatatg aacatcgtcg cccctgcctg
     3001 gggctgccca accgtggcgt acggtcccgg agacgccgca cttgaccaca ctccgcatga
     3061 acacattgaa cttgacgaat acctgaaaag catctctgta ctggagcagg tgctgcggag
     3121 tgcggtggct tgacaccgct ttaaaaccga tacgtcataa atgaataggc aaacgtctgc
     3181 ggtgaacctg gggtcgcggc tttccagaac gcgcctggaa tccagtggcc aaacccgctg
     3241 ccaaacgaga ctccgggccg aaagttgtac tggacttgaa gatcaacttc ctgggcaacg
     3301 tgggcatccg gtgcaccatt tggaaggatt gccgccagtg cac
//
LOCUS       DAOQDO010000439         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21710_length_3342_cov_3.471859, whole genome shotgun sequence.
ACCESSION   DAOQDO010000439 DAOQDO010000000
VERSION     DAOQDO010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21710_length_3342_cov_3.471859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..177
                     /locus_tag="PKZ53_08300"
                     /pseudo
     CDS             1..177
                     /locus_tag="PKZ53_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
     gene            838..2031
                     /gene="aroC"
                     /locus_tag="PKZ53_08305"
     CDS             838..2031
                     /gene="aroC"
                     /locus_tag="PKZ53_08305"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNJ40476.1"
                     /translation="MFRFTTAGESHGKALVTIVEGVPSGLMIETSQINHQLWRRQQGY
                     GRGGRMKIETDAVEVLSGIRHGKALGSPITLLITNRDFANWQTVMASDAIPEWEAIEG
                     DTKKSRQVKRPRPGHADLPGGVKYATRDLRDILERSSARETAARVAAGAVARAVLAPF
                     GIELISHVTMLGGIPSGQRLTSATWEQIAAIQDNTVMRCVDAELEKQMVAAVDDARQN
                     GDTLGGTFEVVVRGLPVGLGSHTRWDARLSARLAQAIMSIQAVKAFEIGAGVEVSRLP
                     GSQIHDEIGYNSETQQYTRLTNRAGGLEGGMTNGQELRVRGHLKPISTLRKPLKSVDI
                     ETKAEVEAAFERSDITAVPAAGVIGEAMVALVLAEAMREKFGGDSIQEMQRNFNTYAD
                     QVAAY"
     gene            2064..2567
                     /gene="def"
                     /locus_tag="PKZ53_08310"
     CDS             2064..2567
                     /gene="def"
                     /locus_tag="PKZ53_08310"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNJ40477.1"
                     /translation="MKLNIVKFGDPILQQKAEPVTEFNAELKELVANMFETMYEAPGV
                     GLAAPQVGVSKRLFVMDCSKDRSRQFVFINPEIVSNEGKQKGDEGCLSFPGIYFAVER
                     PVRVVVSAQDIDGKKFTVDLMELEARCAVHETDHLDGELFINRVGPLKRDIIKGRIKK
                     MMKAGKW"
     gene            complement(2752..3180)
                     /locus_tag="PKZ53_08315"
     CDS             complement(2752..3180)
                     /locus_tag="PKZ53_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ40478.1"
                     /translation="MRTVKELLGSKPMNIWSITPEASVFQALELMAEKEIGALVVLSG
                     SKLVGLLSERDYARKVILKGKASKETRVREIMTDKLITIRPETSIDECMALMTDKRIR
                     HLPVLEGERLVGMISIGDVVKAIISEQKFIIQQLENYITA"
BASE COUNT          862 a          784 c          926 g          770 t
ORIGIN      
        1 gggaaggcgg tcagtctcgc gccaggcggc taccatgtga tgctgatgga cctgaaacag
       61 ccgctgaaaa agggcgacac cgtgaccatc acgctgcagg tcgagggcgc caacaagaag
      121 acggaaagcg tcgaggtcaa ggccgaagtg cgtgacctga ccgcggccgc tccggctgcc
      181 cccgccaaac caaaaccaac ctcaaccaaa ccagctcctg caccaagcaa gccacctgaa
      241 cagccaaaga aagaaattcc agcggccacc aaagtcgctg ttccggctga ttgggatacc
      301 tactgggata gtgaaaaagg atatcagttt gaagttccaa ctggaaccaa agctgaatgg
      361 gtcaccgttg agggtgtgga ttggtttgag gggacgaccc catccaacgt ccaggtaaac
      421 gtggcagctt ataaagataa aaatgcttcg aaagaagatt tattagcgct tgcaaaacaa
      481 ttttgcctcc aggacggcga tacagatgtg gtgattggtg cagccaaaga actcagtggt
      541 gattattccc tggctgaagg cagcggaacc ttaaaagatg gtaagaaata caaaatgaag
      601 attttggtcg cgacagatgt gacagataac tacattgtga ttctgtggac agatgtcgat
      661 aagtatgatg ccaataaaga tatcattgat ggtatctggg gcagcttctc aatgtacagc
      721 ggtgggttca ctggcgaaag ttaaacccaa ccactcgaca gttggtttac aataggggcg
      781 aagaaggtga aatccttctc gcccctattt atttttttca tacaacaacg aggtactatg
      841 ttccgattca caacggctgg cgaatcacac ggcaaggcat tggtgaccat tgtggaaggg
      901 gtgccatccg ggctgatgat cgagaccagc cagatcaacc atcaactctg gcggcgtcag
      961 caagggtatg gtcgtggcgg gcgcatgaaa attgaaaccg atgcggtgga agttttgtct
     1021 ggtattcgcc atggcaaggc cctgggctcg ccaattacct tactgattac caatcgggat
     1081 tttgccaact ggcagacggt gatggcctct gacgccatcc ctgaatggga ggccattgaa
     1141 ggcgacacta aaaaatcacg ccaggtgaaa cgtccgcgtc ccggtcatgc cgaccttccg
     1201 ggtggtgtga aatacgcaac ccgcgattta cgcgatattc tggagcggtc aagtgcgcgt
     1261 gaaacggcag cccgtgttgc tgccggggca gtggccagag ccgtactcgc tccttttggt
     1321 attgaactga tcagtcacgt caccatgctg ggcgggattc catcaggtca gcgcctgacc
     1381 agcgccacct gggaacaaat tgccgctatt caagacaaca cggtgatgcg ttgcgtggat
     1441 gctgaacttg aaaaacagat ggtggcggct gttgatgacg ctcgtcagaa tggcgatacc
     1501 ctgggcggta cctttgaagt cgtcgttcgt gggttgccgg ttggtttggg ctcccatacc
     1561 cgatgggatg cccggttaag tgcccggctg gcacaggcaa tcatgtcaat ccaggctgtc
     1621 aaggcctttg aaatcggggc tggcgtggaa gtctcccgct tgcccggctc gcagattcac
     1681 gatgaaattg gctataattc tgaaactcag cagtacacac gccttaccaa ccgggcgggc
     1741 ggacttgaag gcggaatgac caacggccag gaattgcggg ttcgcggtca cttaaagccg
     1801 atttccacac tccgaaaacc actcaaaagt gttgatatcg aaaccaaagc cgaggtcgaa
     1861 gccgcgtttg aacgctctga catcacggct gttcctgcgg ctggggtcat tggcgaagca
     1921 atggtcgcac tggtgctggc tgaggcaatg cgtgaaaaat ttggcggaga ttcaatccag
     1981 gagatgcagc gaaattttaa cacctatgcc gaccaggtgg ccgcttattg atcggtcggt
     2041 tgaactcact caaaggaaag actatgaagt tgaatattgt gaaatttgga gatcctatcc
     2101 tgcagcagaa agctgagcca gtcactgagt tcaatgcaga attgaaagaa ctggtcgcca
     2161 atatgtttga gacaatgtat gaagcgcccg gagttgggct ggctgctccg caggttggtg
     2221 tttcaaagcg attgtttgtg atggattgct cgaaagaccg cagccgacag tttgtgttta
     2281 ttaatccgga aattgtttcg aatgaaggga aacagaaagg tgatgaaggc tgcctgagtt
     2341 ttccgggcat ctattttgcc gtcgaacgcc cggtgcgcgt ggtggtctca gcccaggata
     2401 ttgacggaaa gaaatttacg gttgacttga tggagcttga agcccggtgt gccgtccatg
     2461 aaaccgatca ccttgacgga gaactcttta tcaatcgggt tggcccgctc aaacgcgata
     2521 ttatcaaagg ccgcatcaaa aagatgatga aagccgggaa atggtaagca ggcaggctga
     2581 agacattggg ctgaagacat tgggctgaag acattgggct gaagacattg ggcttagggc
     2641 tgaagaagtt gggctcaggg tattgaaccc atcttcttca gccccgagct tgcgagtctt
     2701 tagcccccag ccctgagccc aatgttttca gccccaggtt ctccaaaaaa atcaggcggt
     2761 aatatagttt tcgagttgtt gaatgataaa tttctgctca gaaataatgg ctttgaccac
     2821 atctccaatt gaaatcattc ccaccagcct ctcaccttcc aaaaccggca ggtgacggat
     2881 gcgtttgtcg gtcatcaacg ccatacattc atcaatggat gtttctggtc ggattgtgat
     2941 cagcttgtcg gtcataattt cccgaacccg agtctctttc gacgcttttc ctttcaaaat
     3001 cacctttcgt gcataatccc gttccgaaag caggccaacc agttttgacc ctgacagaac
     3061 gacgagcgct ccgatttcct tttcagccat gagttcaagc gcctgaaaga cagaggcttc
     3121 cggggtaatt gaccagatat tcatgggttt ggatcctaac agctctttga cggttctcat
     3181 gaaataactt cctttctctg tggcaggaca cagattgaat gagcgagaag tgggtaaagt
     3241 cgtgacggtt gcccagaaaa ggagggatgg aagtgagatg gaggtgattt tttgattact
     3301 tgctgagtcg gtcggtgacg actgaatgca aaggagcagg at
//
LOCUS       DAOQDO010000440         3332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21838_length_3332_cov_3.599939, whole genome shotgun sequence.
ACCESSION   DAOQDO010000440 DAOQDO010000000
VERSION     DAOQDO010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21838_length_3332_cov_3.599939"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /gene="infB"
                     /locus_tag="PKZ53_08320"
     CDS             <1..1210
                     /gene="infB"
                     /locus_tag="PKZ53_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNJ40479.1"
                     /translation="VVAINKIDKPEINIDRVKKELADHGLLWDGWGGDTVMVEISAKQ
                     RINLESLLEMIILTTDILELKANSKRLASGVVLEARLDRGRGPVASVLVQQGTLNIGD
                     PIIAGLNFGRVRALFDDRGRNVQSAGPAIPVEILGLQGVPKAGDLFQVVTDAAKAQEV
                     ATWRQSKHRIAQLSSSAARGLEDVFEQMKAGKLKELLVILKADVQGSVEVLRDTLEKL
                     SSERVKVRVIRADVGAITESDVLLASASNDMAHTCVIIGFNVRPAPRVSELAKQEKVD
                     IRMHSIIYKVEEEMRKAMIGMLDPTLKEVQLGRAEVRQVIKIPKVSNVAGCMVTDGVI
                     KRTAQVRVLRDNTVIFEGHLSSLRRFKDDTSEVKTGFECGIGVERFNDIKPGDVLEVF
                     TTEKVLPTEL"
     gene            1352..1786
                     /gene="rbfA"
                     /locus_tag="PKZ53_08325"
     CDS             1352..1786
                     /gene="rbfA"
                     /locus_tag="PKZ53_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNJ40480.1"
                     /translation="MGRLGTQSSRSRHQSGSVNHRRERLAESVREEICEIICFEVSDD
                     RIHGVDVTGVELASDLHHARVFVVTTGENPDTALLIDALNHASPFIRHQLAARLNLRK
                     TPQLHFVYDVAYAQALRVEQLLSEEKGTSTPSETTDQSDSEM"
     gene            1975..2943
                     /locus_tag="PKZ53_08330"
     CDS             1975..2943
                     /locus_tag="PKZ53_08330"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNJ40481.1"
                     /translation="MLSQVVELIESKDRFMITSHVRPDGDSIGSSLALYWTLKALNKD
                     VCVIIKDSVPYAYRNLPGTKDILKMPEITQPFDAAFVIECSDIDRPGLIGLEKQFVVN
                     IDHHSTTALFGQINWIDSTAAAVGEMIYNLCKALGVTVNQEIAECVYTALLTDTGSFH
                     FSNTTERTFKIASELVRRGARPAFISHSLFYSNPYTKIKLLGEVLSTLQRDETGRIAW
                     VTMPNHLMNQVGASEDDLDGIVNYPLSVGEVEVVAFFKEMQPDHFRISLRSKGNVNVA
                     KIAEKFGGGGHRNASGCAYKGPLEAAEHQILSSLQRALGITPIAAD"
     gene            3153..>3332
                     /locus_tag="PKZ53_08335"
     CDS             3153..>3332
                     /locus_tag="PKZ53_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40482.1"
                     /translation="MNSKPEIALKSPFPAVPFPKLFTACIAAALVPGAGHFVLGKKGR
                     GMLFLICIYSMVGLGL"
BASE COUNT          855 a          840 c          847 g          790 t
ORIGIN      
        1 ggtggtggca atcaataaga ttgataaacc tgaaatcaat attgatcggg taaagaaaga
       61 actggccgat catggattgt tgtgggacgg ctggggcgga gatacggtga tggtcgaaat
      121 ttcggccaag caacgtatca acctcgaatc cctgctggaa atgatcattc tcacaacgga
      181 tatccttgaa ctgaaagcca attccaaacg gcttgcgtcc ggagttgtcc tcgaagcccg
      241 actggatcga ggccggggtc ctgtggccag tgttctggtc cagcaaggta cgctcaacat
      301 tggagatcca atcatcgccg gcctgaattt tggccgggtg cgggcactct ttgatgaccg
      361 tggtcgaaat gtccagagtg ccggtccggc catcccggtg gaaatccttg ggttgcaagg
      421 tgttccaaaa gccggtgacc tgtttcaagt ggtaacggat gcagccaaag cccaggaagt
      481 cgccacctgg cgacagtcca aacaccgcat tgcacagctt tcaagcagcg cggcgcgtgg
      541 gttggaagat gtgtttgaac agatgaaggc tggcaaactg aaagaattgc tggtgatcct
      601 caaggctgac gtccagggat cggttgaagt tttgcgcgac acccttgaaa aactttcgtc
      661 tgaacgggtg aaagtgcggg tgattcgggc tgacgtcggt gccattactg aatctgacgt
      721 cttgctggct tcagcctcca atgatatggc gcatacctgt gtcattattg ggtttaatgt
      781 ccgcccggca ccgcgtgttt ccgaactggc caaacaagaa aaagtcgata tccgcatgca
      841 cagcattatc tacaaagtcg aagaagaaat gcgcaaagcc atgatcggta tgctggatcc
      901 gaccctcaag gaagttcaac tgggccgcgc tgaagttcgt caggtcatta aaattccgaa
      961 agtcagcaac gtggctggct gtatggtcac agatggagtc atcaaacgca cagctcaggt
     1021 acgtgtgttg cgtgacaaca ccgtgatttt tgaaggccac ctgagttcgt tgcgacgctt
     1081 taaggacgat accagtgaag tcaaaaccgg atttgaatgt ggtattggtg tcgaacgctt
     1141 taatgacatc aagccgggcg atgtgctcga agtttttacc acggaaaaag tgctgccaac
     1201 cgaactgtaa tggcaacacc tccgaatgat gccatcaatt cacctgggtt tggggtctgg
     1261 ggtttgaggt ttggaagaaa aaggtttttt cagccaacga aacctccctc aacctcaacc
     1321 ctttttcaat ttgaaagtca aagatagaag tatggggcgt ttaggaacgc aatcgtcacg
     1381 atccagacat caaagtggtt cagtcaacca tcgccgtgaa cgtctggctg aaagcgtacg
     1441 cgaagaaatc tgcgaaatta tctgctttga agtttctgat gaccggatcc acggagttga
     1501 tgtcaccgga gtcgaactgg cttcagacct gcatcacgcc agggtttttg tggtcaccac
     1561 aggggaaaat cccgataccg ccctgctgat tgatgcctta aatcacgcaa gtccgtttat
     1621 tcgacaccag cttgcggcgc gactcaatct gcggaaaacc ccacaactcc attttgtcta
     1681 tgatgttgct tacgcccagg ccctgcgcgt tgagcagcta ttaagcgaag aaaaaggaac
     1741 atcaacgcct tcggaaacga cggatcaatc cgactctgaa atgtaattca gctctgccgg
     1801 caacagaaac gggtgacttc agggacggac aatccaggaa aaaaagaccg ccagagatcc
     1861 agggctttgg ccaccgcacc ttgtttctga accagagcgt caattgcccc actggtccct
     1921 ccagttggcc agatgttctc tcgctcaaac cttgcgaccg ttcggagatt gcttatgctc
     1981 agtcaggtgg ttgaactcat tgaatccaaa gacaggttta tgatcacatc tcacgtccgc
     2041 cccgacggag acagtattgg atcatccctt gccctctatt ggacactgaa agccctcaac
     2101 aaagatgtgt gtgtcatcat caaagacagt gtgccttacg cctaccggaa tttacccggg
     2161 accaaagata tccttaaaat gcccgagatc acacagccat ttgatgcggc gtttgtgatc
     2221 gaatgcagcg atattgatcg cccagggttg atcggccttg aaaaacaatt tgttgtcaat
     2281 attgaccatc acagtacaac cgctttgttt gggcaaatta actggattga ctcaacagcg
     2341 gcggcagttg gggaaatgat ctataacttg tgtaaagccc tgggggtcac ggtcaatcag
     2401 gaaattgcgg aatgtgtcta cacggcattg ctcaccgata ccggatcgtt ccacttttca
     2461 aataccaccg agcgaacctt caaaatcgcg tcggaactgg tccggcgtgg cgcccgtcca
     2521 gccttcattt cgcactccct gttttactcc aatccttaca ccaaaattaa actgctcggc
     2581 gaagtgcttt cgacgcttca acgggatgaa accgggcgta tcgcctgggt caccatgccc
     2641 aatcatctga tgaatcaggt tggcgccagc gaagatgacc tggatggaat cgtcaactat
     2701 ccactgtcgg tcggagaagt cgaagtcgtc gccttcttta aagaaatgca gccagatcat
     2761 ttccgcatca gcctccgctc aaaaggcaat gtcaatgttg ccaaaattgc tgaaaaattt
     2821 ggcggtgggg ggcatcgcaa tgcctccggg tgtgcttata aaggcccatt agaagccgcc
     2881 gaacatcaaa ttctatcttc cctgcagcgt gccctgggga ttaccccaat tgcagccgac
     2941 tgaccgtgct ccatctgaga aaaagtacct gatggttggt ggctggcctc agaaggccat
     3001 cgctctcatg ttgatgagga gcattagccc gtaaaaccca gcctgacagg aagattgaac
     3061 agagagatcc tttcccggca accaccaaac caccgctctg acaccggctg gtgtcaacat
     3121 tttggttttc tgctccaaaa aaacatcaac gcatgaattc aaaacctgag atagccctta
     3181 aaagtccatt cccggcagtg ccatttccta aattgtttac cgcctgcatt gctgccgcac
     3241 ttgttccagg cgccgggcat tttgtactgg ggaaaaaagg ccggggaatg ctttttctca
     3301 tctgtattta tagcatggtt ggactggggc tc
//
LOCUS       DAOQDO010000441         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21844_length_3331_cov_3.954212, whole genome shotgun sequence.
ACCESSION   DAOQDO010000441 DAOQDO010000000
VERSION     DAOQDO010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21844_length_3331_cov_3.954212"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKZ53_08340"
     CDS             complement(<1..623)
                     /locus_tag="PKZ53_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ40483.1"
                     /translation="MSFAYEYERPSLAVDCVVFGLDIADLKVMLIERGVPPFQGHWAL
                     PGGFVKPEESLDVAAMRELQEETGIEQAFLEQLYTFGDKGRDPRGWTVTVAYYALVNL
                     SDHTVQGATDAHDARWFSVDDLPPLAFDHEKIFQVALDRLRGKLRYQPIGFELLPKKF
                     TLSQLQKLYEIVLKQDLDKRNFRKKILSMGLLIDLNETQKDVAHRAAR"
     gene            complement(719..1096)
                     /locus_tag="PKZ53_08345"
     CDS             complement(719..1096)
                     /locus_tag="PKZ53_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HNJ40484.1"
                     /translation="MPHLTLYYTANLPAQTSFEPLLAQCHSILANVGGVKIGNCKSKA
                     IRIEEFFVADGTQNQAFVHLDLRLLEGRAPELKQDIGKHCLAAIKEAFSELVEHNQVQ
                     ITVEIRDIVRQNYFKIPADSIAP"
     gene            complement(1277..>3331)
                     /locus_tag="PKZ53_08350"
     CDS             complement(1277..>3331)
                     /locus_tag="PKZ53_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40485.1"
                     /translation="VMSQALPPFMPMPQSRPAPAPPGGMMHTGQFQTMTSDVFEKTGT
                     LALPETQEFDLETESEPAGLQKEPEVLFAGLVPVVQGKASLRLLLPDVFADYVVEAFV
                     ISGLDWATTESRFQAKKDPYLSFSLPAFVHPEDTAIGHLNFGATAGNIQLQVRCNGVP
                     VPLAFGGKGLQPGEVLEVQSGEVTFLTGPGVYTATLQQLSTESIETARHQVDAPGVFR
                     RLTRSLVFLEPGKSLSRAEDKGILSMRILPGLEKPFTALIEATADYGHACCEQTAAKL
                     LAACGMYALANGNSAQREKAEAIILAGIRREESMWLPGRGFKMYPESSNSPHDYYGPK
                     AARYLFNLDLLRTGNHLSKPLKQGIEKGLKMAADAAKAYRLNWPLETARSGEDAYNVM
                     RFNSDSLVQGKALDYVRKRVSEGKITEIPMNPYCYGAVLERSESAYAAATLFRSGNRT
                     DWLQALKLANTIVNHLNEQGSLYSTVDSVAAIALMSELKAAQVIGTSSTVKINRRELP
                     TAQAIELAEEVESIEAVDGVVAVEITRLVEENWSTFEAGLPVRISLEKNGRPGRQFVV
                     GDAIDLRVKLEGGYKTGDLLWVCLPESLSRLIGGGQVKLFSLDFREKDELVVPLAATS
                     ITVDSQGNIAPQHYAVCVRNMFEEERAGSPGLLTVTVTPADTTLGTSLVSRALRALRD
                     LI"
BASE COUNT          800 a          861 c          867 g          803 t
ORIGIN      
        1 cgggcggctc gatgagcaac atctttctgg gtttcattga ggtcaatcag cagccccatg
       61 ctgaggattt tcttgcggaa attgcgcttg tcgagatcct gcttgagcac aatttcatac
      121 agtttttgga gttgtgacag ggtaaatttt tttggcagga gttcaaaccc aatcggctga
      181 tagcggagct ttccacgcaa tcggtccaat gccacttgaa aaattttttc gtggtcaaat
      241 gccagtggtg gaaggtcatc cactgagaac caccgggcat cgtgggcatc agtcgccccc
      301 tggacggtgt ggtctgacag gttgaccagc gcataatagg caaccgtgac tgtccagcca
      361 cgcgggtcac gacctttgtc gccaaaggta taaagctgct ccagaaatgc ctgttcaatc
      421 ccggtttctt cctgaagttc acgcattgca gcgacatcga gtgactcttc cggtttgaca
      481 aagcctcctg gcaatgccca gtgcccctga aacggaggca caccgcgttc aatgagcatc
      541 acctttaaat cagcgatatc aagaccgaag acaacacaat caaccgccaa actgggccgt
      601 tcatattcgt aagcaaatga cataggaaga aatgacaaga tgacaagatg gcgaaacgag
      661 gcgctgaaaa aatatccggc ccctgcgatt ttattcctta ttcttcatgg ctggctggtc
      721 acggggcaat actatccgca ggaattttaa agtagttttg gcggacgata tcacgaatct
      781 caacggtgat ctggacctga ttgtgttcca ccaactcaga aaaagcctct ttgatcgcgg
      841 ccagacaatg tttcccaata tcttgtttga gttctggagc gcgaccttcc agcaaccgga
      901 ggtcaaggtg cacaaatgcc tggttttgtg ttccatcggc cacgaaaaac tcttcgatcc
      961 gaatggcttt gcttttgcag ttgccgattt tcacgccgcc aacattggcc agaatagagt
     1021 gacattgagc cagcaatggt tcaaagctgg tttgggcggg taaattggcg gtgtagtaga
     1081 gcgttaaatg tggcatgggg aatcctttgg gaatagcaac tgacagaatg acaactgaca
     1141 gaataacgac ctcgttatcc cagtttgcaa tgaaggtatc gtattatggg aaggctaaat
     1201 tattgaaaaa attgtatttc cctgacccat gaccccgata ccctgacccc aaaatgggat
     1261 taccctggtc atctgatcag atcaagtcac gcagggctcg taaagctcga ctgaccaggg
     1321 atgtacccag cgtggtatca gccggagtga cggtaacggt gagcaggccg gggcttccag
     1381 cccgctcttc ttcaaacata tttctgacac agacggcata gtgttgtgga gcaatatttc
     1441 cctgactgtc tacggtgatc gaagttgccg ccagcgggac aaccagctca tctttttcac
     1501 gaaaatcgag tgaaaataac ttcacctgac cgcctccgat caaccgggaa agtgattcgg
     1561 gcaaacagac ccacaacaaa tcgccagttt tatagcctcc ttcgagtttg acgcgcaaat
     1621 caatggcatc acctaccaca aattgacgac cggggcgacc attcttttca agcgaaatcc
     1681 gcaccggcag gccagcctca aaagtgctcc agttttcctc aaccaggcgg gtgatctcaa
     1741 ccgcaaccac gccatcaacg gcctcgatgg attcaacttc ttcagccagt tcaatcgcct
     1801 gagcggtggg gagttcccgc cgattgattt tcacggtgga actggtgccg atgacctggg
     1861 ccgctttcaa ctctgacatc aaggcaattg cagcgacgga atcaaccgtt gagtacagcg
     1921 acccctgctc gttgaggtga ttgacgatgg tgttggccag tttcaatgcc tggagccaat
     1981 cggtccgatt gccgctccga aaaagtgtgg cggcggcata ggcagattca gatcgttcta
     2041 aaaccgcgcc gtagcaatag gggttcatcg gtatctcagt gatttttcct tctgaaaccc
     2101 gcttgcgtac atagtccagg gcttttccct ggacaagact atctgaattg aaccgcatca
     2161 cattgtacgc gtcttcgccg gacctggcgg tttcaagcgg ccagttcaac cgataggctt
     2221 tggcggcatc cgcagccatt ttcagacctt tttcaattcc ttgcttgagt ggtttgctca
     2281 ggtgattgcc agttcgcagc agatccagat taaacaaata ccgcgccgct tttggtccat
     2341 agtaatcgtg cgggctgttt gatgattcgg ggtacatttt aaacccacgt cccggcagcc
     2401 acattgattc ctcgcgccgg atgcccgcca ggataatcgc ttctgctttt tcgcgttggg
     2461 cgctgttgcc gttggccaga gcatacatgc cacaggcggc gagcaatttg gccgcggttt
     2521 gttcgcaaca ggcgtgacca tagtccgccg ttgcctcgat cagtgccgtg aatggcttct
     2581 ccaaccccgg caaaatgcgc attgacagaa ttcctttgtc ttcggcacga ctaagagatt
     2641 tgcccggttc aagaaaaacc agtgaccgag tgagccgacg aaagacacct ggtgcgtcca
     2701 cctggtgacg ggctgtttcg atgctttcgg ttgaaagttg ctggagagtg gcggtgtaca
     2761 cacctggccc ggtgaggaag gtcacttcgc cgctttggac ttctaaaacc tcaccgggtt
     2821 ggagtccttt cccgccaaat gccagcggga ccgggacacc attgcagcga acctgaagct
     2881 ggatgtttcc ggcggttgct ccaaaattga gatgaccgat agccgtgtct tccggatgaa
     2941 caaaggcggg aagtgagaaa gacagatagg gatccttttt cgcttgaaat cggctttcgg
     3001 tggtcgccca atcaagccca gagatgacaa aagcttcgac gacataatcg gcaaacacat
     3061 ctggaagtaa cagcctcagg ctcgcttttc cctgaacgac cggcaccaaa ccggcaaaga
     3121 gcacttcggg ttctttttgg agtccagccg gttcagactc agtttcgagg tcaaattctt
     3181 gagtttcggg cagggcaagt gtgccagttt tctcaaagac atccgaggtc atggtttgaa
     3241 actggccggt gtgcatcatt ccaccgggtg gtgctggtgc tggtctcgat tgtggcattg
     3301 gcataaaggg cggcagggcc tgggacataa c
//
LOCUS       DAOQDO010000442         3330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21861_length_3330_cov_3.192672, whole genome shotgun sequence.
ACCESSION   DAOQDO010000442 DAOQDO010000000
VERSION     DAOQDO010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21861_length_3330_cov_3.192672"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2626
                     /locus_tag="PKZ53_08355"
     CDS             206..2626
                     /locus_tag="PKZ53_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNJ40486.1"
                     /translation="MVYPACPSSYVSRFLVTFGVFLILLGSIVPVTAHPLGNFTINHF
                     ARLRVSNEQVTIRYVIDMAEIPTFQEIQAADKGPDQQPTAAWLKAYGERVVPQYLAGL
                     VFTVDGARIPLQFQSKSVVLQAGSGGLPTLRIECDLVGKFSGVISGNHQLRFEDTNFT
                     ERLGWREIVVMPETGVSIFNSSAFGSPLTDELKAYPENLITAPLNERIAEFSMTLGAI
                     PAKATALQTREGKSVVQTRDRLAELIAVPEVTPTIALIGLLIATFLGGMHALSPGHGK
                     AVVGAYLVGARGTARHAAFLGLTVTITHTAGVFALGMITLFASQFILPERLFPILSLI
                     SGLIVVGVGLRMVLSRARLAMAGPGHSHDHHHDHHHDHHSHDHHDHDHSHAHHHHGIG
                     VHTHDGHTHSHLPPGADDEPVTWRSLLALGISGGLLPCPSALVLLLSAISLHRVGYGL
                     LLVLAFSFGLAGVLTVIGLAFVYAGRFFESKTKESTNGTIADLSRLALRYTPVLSAVV
                     VTCLGIIICIQTLPDFKSLAGPSASPVIQSPSQVDEPEHGPSLVGLSALGVLGLGLVF
                     GLKHATEADHVVAVSTIVSEHKSLGRAAIVGGLWGIGHSTSLVVVGLLVLVLRMFIPD
                     RIAPWLDWASPWLEFGVALMIIGLGANALSKALRKRSDVHIHTHTHDGSPHSHIHFHD
                     PDTDHEQAHAHHLHFVKQIGLKPLLVGAAHGLAGSAALTLLVLSQIPSPVLGLVYLVV
                     FGAGSIGGMLAMSGLVGIPFILSAAWINRFHHVLQVAASLFSILFGVWYAAQNDVVQG
                     ILATVLGT"
     gene            2718..3185
                     /gene="nikR"
                     /locus_tag="PKZ53_08360"
     CDS             2718..3185
                     /gene="nikR"
                     /locus_tag="PKZ53_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNJ40487.1"
                     /translation="MGELVRFGVSIEDELLEKFDQLISARGYATRSESLRDLIRDALV
                     QARLESRSAEPEQEVVGSLTFVYDHHARNLATQMAEKQHANHDIVVSVTHVHIDHHDC
                     MEVLLLRGSVSRVRALADSILNLKGIKHGKLFVTFPSHSIEKHHHHSHSHKAL"
BASE COUNT          721 a          827 c          864 g          918 t
ORIGIN      
        1 caggccacca ttgcccaaaa agcattggcc gaagcggcct gagttcaggg aaaaaaatca
       61 gaaaagcctc acctgcggtt tgagagtact tcgttcccat aaaatatctc ctgatttttg
      121 aatggccttc attgcttgaa agtgtgctgt tttcccaatc gctgcggttg ataaaatctg
      181 ttctatttaa acccttgagt taattatggt ttatcctgcc tgtccgtcga gttatgtcag
      241 tcgttttctg gtgacgttcg gtgtcttttt gattcttttg ggctcaattg ttccggtgac
      301 tgcccacccg cttggtaatt tcaccatcaa tcattttgcc cggctacggg tcagtaacga
      361 gcaagtcacc attcgctatg tgattgatat ggctgaaatt ccaacctttc aggaaattca
      421 ggcggctgac aaaggaccgg accagcagcc aacagccgcc tggttaaagg cgtatgggga
      481 gcgtgttgtc ccgcaatatc tcgccgggct ggttttcacc gtggatggag cccggatccc
      541 gcttcagttt cagtccaaat cagttgtact ccaggccggg agtggtggat tacccaccct
      601 gcggattgaa tgtgatctgg ttgggaagtt ttcaggggtg atttctggca atcaccagct
      661 ccggtttgaa gacacaaatt ttactgagcg gctcggatgg cgtgagatcg tggtgatgcc
      721 tgagaccggg gtatcaattt ttaatagctc ggcctttggt tcacccttaa ccgacgaatt
      781 aaaagcctat ccagagaacc tgattacggc accgctcaat gagcgaatag ccgaattttc
      841 aatgacttta ggtgctatcc ctgcaaaggc aaccgcgttg caaacccgtg aaggaaaatc
      901 agttgtccaa acccgtgatc ggctggctga actgatcgct gttcctgaag taaccccaac
      961 cattgcgttg attggcttat tgatcgcgac ttttttaggc gggatgcatg cgctttcgcc
     1021 tgggcatggt aaggccgttg ttggggcata tctggtcgga gcgcggggaa ctgcccgcca
     1081 cgctgctttt cttggactca cagtcaccat tacccatact gctggcgttt ttgccctggg
     1141 aatgataacc ctttttgcct cgcaatttat cttacctgag cggttgtttc cgattttgag
     1201 tttaatttca ggactcatcg tcgtgggagt tggactcaga atggttttga gtcgggctcg
     1261 attggccatg gctggtccag gccattcaca cgatcaccat cacgatcacc atcacgatca
     1321 tcattcacac gatcatcacg atcatgacca ttcacacgct catcatcacc atggcatagg
     1381 agtgcatacc cacgatggcc acacgcactc acacctcccg ccaggggctg atgacgagcc
     1441 agtgacctgg cgcagtttgc tggcacttgg tatttcgggt ggattgcttc cttgcccttc
     1501 ggcgctggtt ttattgctct cagctatttc cctgcatcga gttgggtatg ggttgctgct
     1561 ggttttggca tttagtttcg gattggcggg tgttttgact gtcattggtc tggcatttgt
     1621 gtatgccggg cgcttttttg aatcgaaaac aaaagaaagt accaatggaa caatcgctga
     1681 tctgagccgc ctggccttgc gctacacccc ggttttgagt gcggttgttg tcacctgtct
     1741 ggggatcatc atttgtattc aaacactccc agattttaaa tcgctggcgg gtccatccgc
     1801 atcgcctgtg attcagagtc cttcccaggt tgatgagcca gagcatggcc cctccctggt
     1861 tggtctcagc gcgctgggag tcctgggatt agggctggtt tttggcctca aacacgccac
     1921 tgaagctgac catgtggttg cggtttcaac cattgtgagt gaacacaaaa gtctggggcg
     1981 ggccgctatc gttggcggat tatggggaat tggtcactcg acctcactgg ttgtggttgg
     2041 tttgctggtg ctggttctgc gaatgtttat tccagaccgg attgcgccct ggttggattg
     2101 ggcttcaccc tggcttgaat tcggcgtggc tttgatgatc attgggcttg gcgccaatgc
     2161 gttatccaaa gcactccgaa aacggtcgga tgttcatatc catacccata cccatgacgg
     2221 atcaccgcac tcacacatcc atttccacga tcctgacact gatcacgaac aagctcacgc
     2281 gcaccatctc cattttgtaa agcagattgg gttaaaacca ttgctggtcg gcgccgcgca
     2341 tggtctggcc ggatcggctg ccctgacact actggtactg agccagatcc cctcaccggt
     2401 tcttgggtta gtgtatctgg ttgtctttgg tgccgggtca atcgggggaa tgctggcgat
     2461 gtcggggctg gttggaattc catttattct cagtgcggcc tggatcaatc gtttccacca
     2521 tgtcctgcaa gttgccgcca gcctgtttag tattctcttt ggtgtctggt atgcggcgca
     2581 aaacgatgtg gttcagggga ttttggcaac ggtgcttggg acgtaaaacc tggggctaag
     2641 ggcttggggc tgagggtttg gaacccaatc tctttcaata atgaagaacc aaaaaccagg
     2701 cacgaataag gtgaactatg ggtgaacttg tccgatttgg cgtcagcatc gaagacgaac
     2761 tgcttgaaaa atttgaccag ttaatcagcg ctcgtgggta tgccacccgc tctgaatcgt
     2821 tgcgtgattt gatccgagat gcgctggtgc aggcccggct ggaatcacgc agcgcagagc
     2881 ctgaacagga agttgttgga agtttaacct ttgtctatga ccaccatgcc cgaaatctcg
     2941 cgacccagat ggctgaaaag cagcatgcca accacgatat tgtggtttca gttacccacg
     3001 ttcacattga ccatcatgat tgtatggaag tgctgttgct tcgtggcagt gtcagtcggg
     3061 tccgtgccct ggctgattca atcttgaatt taaaaggaat caagcacggc aagttatttg
     3121 tcacctttcc atcccattcg attgaaaaac accatcatca ttcccactca cataaggcgt
     3181 tgtaacgaaa gaaaggcgac tgacccggtc agccaaattc cctcctcctc tctatcagga
     3241 aagactaaat cagttcacac tggattggaa cacgtgtgat tgatttcaga ttcagaccat
     3301 caggttgagt ttcaataagt caccagccag
//
LOCUS       DAOQDO010000443         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_21963_length_3321_cov_4.876301, whole genome shotgun sequence.
ACCESSION   DAOQDO010000443 DAOQDO010000000
VERSION     DAOQDO010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21963_length_3321_cov_4.876301"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKZ53_08365"
     CDS             <1..288
                     /locus_tag="PKZ53_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40488.1"
                     /translation="PIVLFFAKPFFDVAVNGNGIVFAPVMTEHRIYRIGQPVESLTSI
                     AGGTPGYAGDNGPATEAAFSSPSAVAIDGANNLYIVDSGNNAVRVIKNGAR"
     gene            411..>3321
                     /locus_tag="PKZ53_08370"
     CDS             411..>3321
                     /locus_tag="PKZ53_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007188987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ40489.1"
                     /translation="MNRTSCRNSWPLGFFVLVCGSLLFTFIGNVHPQNFPQGSQAVGR
                     TEPKIIVPFVVTDAGPFMFYTDTDHDGMSDACETASGLDPNNPVDADGDLDGDGLTNG
                     DECALGTLPTNADTDGDGFNDGIEVQLGYDPKDPSSFPPPNGPQLVSLDVTPRSIGLS
                     VGTVIPPQPVQLSVIGTFDTGETLDLTANPATRFESLNDSIAAVDPSGKVIGIAPGST
                     TIRVRNAGLASDTLIAVTSITESVVGNIKLPGSANNVEVKNQVAYVACGLAGMVVVDA
                     RQPATPQVIATVNLDGSANDIRIVGDFAYLACAEGGLSVVNISDPTNPQVVATMPFSD
                     AVDLMVKGEYAYVAAGKNGLKIINIASPYSPYELATFPLSDPATQVANGVDVDEARQL
                     AYLAVRNGGLLVIDVSNPSAPHLRGAVETGPVNLDVAAMGSTALIAGRSLFFSENGLL
                     SVNTQNPDAPQIAGIASLDNTILTDLEVRGNLIFAAANSTLVNFIPIFTLPENQQIPI
                     WSGVIQASNPNQPDDINAYRFSTGIAVDESFTYLTASSINANQNQRTGESRLIISRYN
                     QTDRNDVAPQAAISSPTADLTVIGGETFPITVSASDDIAVVGVEIKVNGQVAANLTEA
                     PFQLSYTVPNAAPGTQLTFEARAIDSAGNVGEAAPVTISIAPDPLTTVIGRVVTPFGT
                     SVANAQVTLHSDNQATVTDAGGFFEFVGVPTLPFGKAVSASVEEPIEGYQGRSAWVSV
                     VRGGTTDLGLITVSQTKVTLLPSDVTSVVTWNFGGDGPDDLFIGFPDQASQIYFSSGT
                     GSFSFDSRFIMPFGATLSAEFGQFNTSRDLEFDILAQPVGQPQQATTVYLDRDLGVVG
                     NDPFETGLSQECEAMARGIDFVRIGGVQQVYAFLKVTGGTALTVRVEGADRTFGPLIV
                     IPVQSSTSLRALKVLDVNQDDLVDVLVIAEDRDNQRRLLVFPRTSATTFGTPIESE"
BASE COUNT          728 a          900 c          889 g          804 t
ORIGIN      
        1 ccaatcgttt tatttttcgc caaaccattt tttgatgtag cggtgaatgg gaacggaatc
       61 gtgtttgctc cagtgatgac cgaacaccgg atttaccgaa tcggacaacc agttgagtca
      121 ctcacttcca tcgctggagg aacacctggc tacgccggtg acaacggacc agccacggaa
      181 gcggcttttt cgtctccatc agcggtggcg attgatgggg cgaataatct ctacattgtg
      241 gattcaggaa acaatgctgt tcgggtgatc aaaaacggcg cccgataaac accgttgttg
      301 tgaagcaaca ccattttaga ttcaggatca aggaaatctc attttttcaa tcatttcgcc
      361 tgttgttgat gccaggcgat ggtttgaaaa aggcttaaaa aagggaactt atgaatcgca
      421 cgtcgtgtcg gaactcctgg ccgcttgggt tttttgtgct tgtttgcgga agtttgcttt
      481 tcactttcat tgggaacgtc catccacaga acttcccaca aggctctcag gcggttgggc
      541 gaaccgagcc aaaaatcatt gtgccatttg tggttacaga tgccgggcct tttatgttct
      601 ataccgacac cgaccatgac ggcatgtctg atgcgtgcga gacggccagt ggactcgacc
      661 cgaacaaccc ggtagatgcc gatggcgacc tcgatggcga tggcctgacc aatggggatg
      721 aatgcgcact cggaacgctc ccgaccaatg ccgatactga tggcgatgga tttaacgacg
      781 gaattgaagt ccagcttggc tacgatccca aagatccgtc gagcttcccg cctcccaatg
      841 ggccacagct tgtttccctg gacgttacgc cacgttcaat tgggttgagt gtcgggacag
      901 ttatcccacc acaaccggtc cagctttcgg tgatcgggac gtttgacacg ggcgaaacgc
      961 ttgatctgac agccaatccg gccacccgat ttgaatcact gaacgattcc attgccgccg
     1021 tagacccttc aggcaaagtc atcggcatcg cccctggttc aacgaccatc cgggttcgga
     1081 atgccggttt ggcctccgat acgctcattg ccgtgacctc catcaccgaa tcggtggtgg
     1141 gcaatatcaa acttcccggc tcagccaaca atgtcgaagt caaaaatcag gttgcctatg
     1201 tcgcctgtgg tctggctgga atggtggtgg tggatgcccg ccaaccggca actccacaag
     1261 ttatcgcaac ggttaattta gacgggagcg ccaacgacat ccgcatcgta ggtgattttg
     1321 cctatctggc ctgtgccgaa ggtggcctgt cggtggtcaa tatctctgat ccgaccaacc
     1381 cacaggtggt ggcgaccatg ccgttcagcg atgcggtgga tctgatggtc aagggtgagt
     1441 atgcttatgt tgcggcgggt aaaaatggcc tcaaaatcat caatatcgct tctccttatt
     1501 caccctatga gcttgctacc ttcccgttgt cggatccagc cacccaggtt gccaacggag
     1561 ttgatgtgga cgaggcccgt caactggcgt atctggccgt tcggaatggt ggcctgctgg
     1621 tgattgatgt ttccaatccg tcagcaccgc accttcgtgg cgccgtcgaa accggaccag
     1681 ttaacctcga cgttgcggcg atgggttcaa ccgcattgat cgccggcaga tcgctctttt
     1741 tctctgaaaa cggattgctt tccgtcaata cccaaaaccc cgatgctcca caaatcgctg
     1801 ggatcgcctc actcgacaac acgattctga ccgacctgga agtgcgcgga aacctcatct
     1861 tcgcggcggc caattcaacc ctggtgaatt ttatcccgat tttcaccttg cctgagaatc
     1921 aacaaattcc catctggtcg ggagttatcc aggcatcaaa ccccaaccag ccggatgaca
     1981 tcaacgccta tcggttctcg accggaatcg cggtggatga gtcattcact tacctgacgg
     2041 cttcttcaat caatgccaac cagaaccagc gaaccggaga atcccgactc atcatcagcc
     2101 gatacaatca gacggatcgg aatgacgttg caccgcaggc ggcaatttcg tcgccgacgg
     2161 ctgacctgac ggtgattggc ggggaaacct tcccgattac ggtttcagcc tcggatgaca
     2221 ttgccgtggt cggggtcgaa atcaaagtca acggccaggt tgccgcgaat ttgaccgaag
     2281 cgccgttcca actgtcctac accgttccga atgccgcgcc gggaacgcaa ctcacgtttg
     2341 aagcccgagc gattgactcc gccgggaatg tcggggaagc cgcaccggtg acgatttcca
     2401 ttgctccaga tccattgacg accgtgattg gaagagtggt gaccccgttt ggaaccagtg
     2461 ttgccaatgc ccaggtgacg ctccacagcg acaaccaagc tacggtcact gacgctggcg
     2521 gcttctttga gtttgtcggt gtgcctactc tgccctttgg aaaagcagtc tcggccagtg
     2581 ttgaggaacc tatcgagggc taccaggggc gttccgcctg ggtttccgtc gttcgcggag
     2641 gcacgaccga tcttggcctt atcacggttt cccaaaccaa agtcacgttg ctgccatcgg
     2701 atgtgacgtc ggtggtcacc tggaattttg gcggggatgg accagatgac ctcttcatcg
     2761 gattccccga ccaggccagt cagatctatt tcagttccgg aactggttca ttcagttttg
     2821 attcccggtt catcatgccg tttggcgcga cgctcagtgc tgagtttgga cagttcaata
     2881 catcccggga tcttgaattc gatattttgg cccaacccgt tggccagcct caacaagcaa
     2941 ctaccgttta tttggatcgc gatcttggcg ttgttggaaa cgatccgttt gaaactggtc
     3001 tgagtcagga atgtgaggcg atggctcgtg gaattgattt tgtccggatt ggcggggttc
     3061 agcaggtgta tgcgttctta aaagtcaccg gcggaacagc attgactgtt cgtgtcgaag
     3121 gggcggaccg aacctttggc ccgctgattg tgattccggt tcaaagttca acctcgctcc
     3181 gagccctcaa agtacttgat gtgaatcagg atgacctggt tgatgtgctg gtaattgctg
     3241 aagataggga caaccagcgc cgactgttgg tgttcccccg gacttcggcc acgacgtttg
     3301 gaactccgat tgaatcggaa a
//
LOCUS       DAOQDO010000444         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22029_length_3314_cov_3.260816, whole genome shotgun sequence.
ACCESSION   DAOQDO010000444 DAOQDO010000000
VERSION     DAOQDO010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22029_length_3314_cov_3.260816"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKZ53_08375"
     CDS             complement(<1..456)
                     /locus_tag="PKZ53_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40490.1"
                     /translation="MLAAAPKINRRYLVLACALCLGILAIPIALAFRSHPAASLLAQI
                     PTLKTTQAAPRTAQIKTGEVKKSLILDGELRAVRAKTIFAPATNQTKILYLPPEGSLV
                     KVGDKLVEFDGSAIVTRIKEVETQLVSAENRVVELKGQQESALRDMEIEL"
     gene            complement(554..1798)
                     /locus_tag="PKZ53_08380"
     CDS             complement(554..1798)
                     /locus_tag="PKZ53_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40491.1"
                     /translation="MIENNKPSLKSSARQARLGRIWVVFLKEMRETMRDHRVLFGVIV
                     SPLLITPLLLGGMGFFISKKQAEVKAEKLPVAVVGADSVPELITILKEDTTLTLISLP
                     SLSEAEQQVKKHTQRAAFVIPESAKSGLANGQKVELELLYDLSNEKSRTAKSRLEDGL
                     KKFNEQVVVARLKARSLDAGILKPTEAKSRSLAEDSSVGMLILSLILPYTIVLTGALG
                     GMTSAFDICAGEKERGTMETLLVSPVSRTEIILGKILTITSVSMLATLCAIAGLVFSF
                     QSGIKAFEEFTRGKIAVSYSAIGVMIIMMVPLVLFTSVLLLVISSFARNQKEAQAYVL
                     PFMVLLIVPAMLTMVLGEESPLGMSVVPILNTALVLKQVLLSLIKPDFIALTFGSSLL
                     YSIIALVVVVALFNREEILFRS"
     gene            complement(1791..2534)
                     /locus_tag="PKZ53_08385"
     CDS             complement(1791..2534)
                     /locus_tag="PKZ53_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ40492.1"
                     /translation="MIQAKNLVKTFRDRKRGEVHAVNGVSFEAKPGKIFGLLGANGAG
                     KTTTLRILATLLEPTTGEAYVAGHDVRLEPEKVREKIGFLSTSTALYGRLTAREMVEY
                     FGRLYGMPEPLLQQRLKEVFSVLEMNEFANGRCDKLSTGQKQRVSIARSIIHEPPVMI
                     FDEPTTGLDVMTSRTIMRFIERCREQSKTVIFSTHIMSEVERLCDEVAIIHDGRIVAQ
                     GTVEELRERTGLVPLESVFLKLVGASDHD"
     gene            complement(2702..>3314)
                     /locus_tag="PKZ53_08390"
     CDS             complement(2702..>3314)
                     /locus_tag="PKZ53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ40493.1"
                     /translation="VGGTEEIPVDTRVVAATNRNLSQMVEEGTFRKDLFYRVSVIPIE
                     IPPLRERRDDIRELADHFLKKYARMVDRPITRISEEALRYLEKYDWPGNVRELESAIE
                     RAVAFESTSEIRPERLTETILKYNPAKITSAFDLPADGINLEGFIAEIEKSYILQALR
                     RTHGNQTRAAELLKMSVRSLRHLLDKHKIRQTASLLRDTSESN"
BASE COUNT          810 a          827 c          848 g          829 t
ORIGIN      
        1 caattcgatt tccatatccc gcagggctga ttcctgttgt cctttgagtt caaccacccg
       61 attttccgca ctgacaagct gggtttcgac ttccttgatc cgggtaacaa tggcacttcc
      121 gtcaaattcc accagtttgt cgccaacttt gaccaggctg ccttcaggtg gaagatagag
      181 gatctttgtc tggtttgtcg ctggagcaaa aatggtttta gctctcacgg cacgcagttc
      241 tccgtccagg atcagactct tttttacttc accagttttg atctgagccg tgcgaggtgc
      301 cgcctgagtg gttttgagag ttgggatttg agccaggaga gaggcagcag ggtgtgaacg
      361 aaatgccagg gcgatgggaa ttgccagtat cccaaggcac agggcacagg ccaaaacaag
      421 ataccggcga ttgattttgg gtgcagcggc aagcacaacc agacctccgt ggattttctc
      481 gtaccgggtt cttggtgact ggtgcttagg aaatttattg gttcctaagc accaggcacc
      541 agtcaccagg atttcaggat cgaaacaaaa tttcctcacg attaaacagc gccaccacca
      601 ccaccagggc aatgattgaa tagagcagtg aggaaccaaa cgtgagcgca ataaagtcgg
      661 gtttaatcaa gctgagcaag acctgcttga gcaccagtgc cgtgttgaga attggcacca
      721 cgctcatacc gagcgggctt tcttcgccca acaccatagt cagcatcgcc ggaacaatca
      781 aaagcaccat aaatggaagg acataggcct gggcttcttt ttgatttcga gcaaagctgg
      841 aaatcaccag gagcaatact gaggtaaaca gcacaagcgg caccatcatg atgatcatca
      901 ccccaatcgc agagtaggaa acggcgattt taccgcgagt gaattcctca aaggctttga
      961 ttccgctttg aaatgaaaaa accagcccag caatggcaca cagcgtggcc agcatactga
     1021 cactggtaat cgtcaatatt ttcccaagga taatttcagt tcgtgagact ggcgaaacca
     1081 gaagtgtttc catggtgccg cgttcttttt ctccggcgca aatatcaaac gcactggtca
     1141 tcccacccag ggcaccagtc aaaacaatgg tgtagggcaa gatcaggctg agaatcagca
     1201 tgccgacaga ggaatcttcg gccaggctgc gggatttagc ctcggttggc ttgagaatac
     1261 cagcatcaag gctacgagct ttgagtcgcg ccaccaccac ctgttcgttg aattttttga
     1321 gcccatcctc cagtcgactt tttgccgtgc gggatttttc attcgacagg tcatacaaca
     1381 gttccagttc aactttttga ccatttgcca gaccagattt ggcggactct ggaatcacaa
     1441 atgccgctcg ctgggtgtgt tttttgacct gctgctcggc ttcactcaag gatgggagcg
     1501 aaatcagggt cagggtggtg tcttccttca aaatggtaat cagttcagga acagagtcag
     1561 caccaaccac cgcgactgga agcttctcgg ctttgacctc ggcctgtttc ttactgatga
     1621 aaaaccccat gccacccagc aacaatgggg tgatgagcag tggcgagaca atcacaccaa
     1681 agagcacacg gtgatcgcgc atcgtttcgc gcatttcttt gagaaagaca acccagatcc
     1741 gtcccagtcg agcctgccgg gctgacgatt ttaatgaagg tttgttgttt tcaatcatga
     1801 tcagaggctc ctacgagttt caaaaagacc gactctaatg gaacaagtcc ggtacgttca
     1861 cggagttctt caaccgttcc ttgagccacg atgcgaccat catggataat tgccacttcg
     1921 tcacagaggc gctcgacttc gctcatgatg tgggttgaga aaatgacggt tttgctctgt
     1981 tcccgacacc gttcgataaa gcgcatgatg gtgcgggagg tcatcacgtc caaacccgtc
     2041 gttggttcat caaaaatcat gactggcggt tcgtgaatga tggatcgggc gatggaaacc
     2101 cgctgctttt ggccagttga gagcttgtcg cagcgtccat tggcaaattc attcatttca
     2161 agcactgaaa atacttcttt gaggcgttgc tgaaggaggg gttccggcat tccatacagc
     2221 cgcccgaaat attcgaccat ttcgcgggcc gtgagtcggc catagagtgc ggtgctggtt
     2281 gaaagaaaac cgatcttttc gcggactttt tcaggttcga ggcgcacatc gtgcccggcg
     2341 acgtaggctt caccggtggt tggttcgagt aatgtggcca gaattcggag agtcgtggtt
     2401 tttccggcgc cattggcgcc aaggagtcca aagattttgc caggtttggc ttcaaaactg
     2461 acaccattga ccgcatgcac ttctcctcgc ttacggtcac gaaacgtctt cacgaggttt
     2521 tttgcttgta tcatgttgat tccattgttg ttgtgacaaa agtactgaag tggaaagcag
     2581 atcgcttttg gtgctggtgg ggttaactgc cctgaccgta acctttccag ggtagaagaa
     2641 aggccatcca ttgaacgaat tacacggctc ttgaatggca atgtgatctg cgcaccagac
     2701 actaattcga ttctgaggta tcgcgaagca gactggccgt ctgccggatt ttatgtttgt
     2761 cgagcaagtg ccggagagaa cggactgaca tctttaaaag ttcagccgcc cgggtctgat
     2821 tgccatgcgt tcggcgcagg gcttgaagga tgtaagattt ttcaatttca gcgataaatc
     2881 cttccaggtt aattccatct gccggcaggt caaaagctga agtaatcttg gcgggattgt
     2941 atttcagaat ggtttcggtc agccgctcgg gacgaatctc ggacgttgat tcaaacgcca
     3001 ccgcccgctc aatcgcactt tccagttcac gtacattgcc gggccagtca tatttttcaa
     3061 gatagcgcag ggcttcctca gaaatgcgag taatcggacg atccaccatg cgggcatatt
     3121 ttttgagaaa atgatcggcc agttcacgga tgtcatcacg tcgctctctc aagggaggaa
     3181 tctcaatcgg aatcacgctc acccggtaaa acaaatcttt gcgaaaggtc ccttcttcaa
     3241 ccatctggct caggttgcga ttggtggcgg caaccactcg ggtatcaact gggatttctt
     3301 cagtgcctcc gacc
//
LOCUS       DAOQDO010000445         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22049_length_3312_cov_3.706478, whole genome shotgun sequence.
ACCESSION   DAOQDO010000445 DAOQDO010000000
VERSION     DAOQDO010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22049_length_3312_cov_3.706478"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKZ53_08395"
     CDS             complement(<1..651)
                     /locus_tag="PKZ53_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40494.1"
                     /translation="MSDENQSSSGPGAFGRVFRRRQQLPDGQSQGWWSTSRRKFIIGG
                     SVAALGLAGVGTYWLLRDDTSEIDDDSLALQQKHGWNLGSEDKPLTLTGIQPQDSLKT
                     EEWKHYLDFNAMLGAFQPAAPAWAPFFVPTLIQSLKFDTLRGQLHPISTPDMEESYQR
                     GQTIGKDFLANAQNRNETALIADLPGRDAVAFGAGLADKARLVTTFDNFPHPLGVTP"
     gene            complement(660..1934)
                     /locus_tag="PKZ53_08400"
     CDS             complement(660..1934)
                     /locus_tag="PKZ53_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNJ40495.1"
                     /translation="MIQLSPLPLSFPTYFDFAQAIYNTGILSDPWIDGRERFSLQGVI
                     LPQNQADALEEAAERVAYLHQELVEILLNRPTLMSDFYELTPFQQAMWESSGGLWHGI
                     ARADLFVLPDGHIQCCELNSDTPSGEPEAVLLNQLLHAAHGAVIDPNARLEASFLRML
                     RESHAKRTDQPLSSVAIIYPTELTEDLSMILLYTRWLQAAGINVVTGSPFNLRRTRRG
                     IEVLGHPVDLIIRHYKTDWWGERDPVWADAEDFPDPEPLFEPLGMLISAEMAGEVTLV
                     NPLGSVITQNKLSLAFFWEYRSLFSEMAQTWIRTYLPETHRLSYFSIDRLRKEQSQWV
                     LKSDYGCEGAETICGPFVSPETWQRSLDLAIPQHFVAQRFFEAQPDDQGRIPNYGVYL
                     IGGSASGYFTRLSSQSTEYSALTVPTFLSPEE"
     gene            2148..>3312
                     /locus_tag="PKZ53_08405"
     CDS             2148..>3312
                     /locus_tag="PKZ53_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ40496.1"
                     /translation="MGEKEMKNTYLEQVKAEQQQVEVDLRAKRGRQQEMAVPNRAAMA
                     APLAPQAMAGPPADFEMAFVQSSPALAPALDVRVVGFWRWKTVIVPPNAYVVHTRRGH
                     SDPLHIGLGISFGFNPYSDSYLVVPSAMQTILINANCICRELQGLLVQGYVQWIIGDF
                     ATAYKKLDFSDLVDPMRIVNVQLREQAEAAIKDKVATMSIDDVLSDKQPIIQELTARL
                     RQVAEGEGDSDKGLGLRIVTVQIKEAVVSSTRLWENLQEPFRADRARIANLAKLEAES
                     VIKAEELKHEQTRSVTEMESKSELARIRAAKEAEVFDREQAEKARRHQKEQEVTRATL
                     LERNITLKQEQKSKRELEEATIENSIALAKANAKLEQERLEQEFARERLKLTEM"
BASE COUNT          818 a          834 c          930 g          730 t
ORIGIN      
        1 aggggtcacg ccaagtggat gcggaaaatt gtcaaaggtc gttaccagcc gggctttgtc
       61 agccagtccg gcaccaaatg ccaccgcatc ccgtcccggc aggtcggcaa tcagggccgt
      121 ttcatttcgg ttttgggcat tggcgaggaa atctttcccg atggtttgac cacgctgata
      181 gctttcttcc atatcgggcg ttgaaatcgg gtgcaactgc ccccggagcg tgtcaaattt
      241 gagcgattgg atcagcgttg gcacaaaaaa tggcgcccag gccggtgccg ctggttggaa
      301 ggcgcccagc atggcattga agtcaagata gtgcttccac tcttcggttt tgagcgagtc
      361 ctggggctga atacctgtca gggtcaatgg tttatcttct gatcccagat tccagccatg
      421 tttttgctgc agggccaggg aatcgtcatc aatttcactg gtgtcatcgc gaagcagcca
      481 gtacgttcca acgcctgcca gcccgagagc ggcaacgctg ccgccaataa tgaattttcg
      541 acgtgaggtg gaccaccagc cttgtgattg accgtccgga agttgctggc ggcggcgaaa
      601 tacacgacca aatgctccag gtccggatga agattgattt tcgtctgaca cgattgacat
      661 tactcctctg gggaaagaaa cgttggcact gtcaacgctg agtattcagt gctttgcgat
      721 gagagccttg taaaataccc acttgcactg cctccgatca agtacactcc gtagtttggg
      781 atccgtccct ggtcatcagg ctgggcctca aaaaaccgtt gagccacaaa atgctgggga
      841 attgccagat ccagcgacct ttgccaggtt tcaggtgaca caaatggtcc gcaaattgtt
      901 tcagcgcctt cacagccata atcacttttc agcacccact gcgactgttc tttccgcagt
      961 cggtcaattg aaaaatagct cagccggtgg gtttccggca ggtaggtccg gatccacgtc
     1021 tgagccattt ctgaaaacag cgaccggtat tcccaaaaga acgccagcga cagtttattt
     1081 tgggtgatga ccgaaccgag cggattgacc agcgtgactt cgccagccat ttcagccgaa
     1141 atgagcatgc cgagtggttc aaacaggggt tccgggtcgg ggaagtcctc cgcatcagcc
     1201 cacaccggat ctcgctcgcc ccaccagtcg gttttgtaat gccggatgat caaatcaacg
     1261 ggatggccca gaacttcaat tccacgccgg gtccggcgca gattaaatgg agagccagtc
     1321 accacgttga ttccggcggc ctgaagccag cgggtataga gcagaatcat tgacaggtct
     1381 tcggtgagtt cggttggata aataatcgcg accgacgaaa gcggctgatc ggtgcgtttg
     1441 gcgtggcttt cacgcaacat tcgcaggaag ctggcttcaa gccgggcatt cggatcaatc
     1501 actgcaccgt gggctgcgtg caaaagctga ttgagcaata cggcttcggg ttcgccagac
     1561 ggggtatcgc tgttgagttc acagcattgg atgtgcccgt cggggagcac aaacaaatcg
     1621 gcgcgggcaa tcccgtgcca gaggccgccg gatgattccc acatcgcctg ctgaaatggg
     1681 gtgagttcat agaaatcact catcaaggtg gggcgattga gcaggatttc aaccagttcc
     1741 tgatgcagat acgccacccg ttcggcagct tcttcgaggg catcagcctg gttctgaggc
     1801 aggataaccc cttgtaatga aaagcgttca cggccatcaa tccaggggtc agacagaatt
     1861 ccggtgttgt aaatggcttg tgcgaagtca aagtaggttg gaaagctgag tggtaatggt
     1921 gaaagttgaa tcataaggca aatcagttca ttgtcggtaa aagaatgcag aagaaatatc
     1981 acaggccgaa tctatcatct ggtgaatgaa agaaacaaat tgcattctga ccagatctct
     2041 tcagaatatg aattcagagg gtgtccttga ctgatttacc gtgttcagag ttccggtttc
     2101 aggtgatact ggtttggata ggcaggtttt tttgacagga gcttgttatg ggggaaaagg
     2161 aaatgaaaaa cacatatctg gagcaggtca aagccgaaca gcagcaagtc gaagtggatt
     2221 tacgcgccaa acgtggacgt caacaggaaa tggctgttcc caatcgagcg gccatggcgg
     2281 caccacttgc cccgcaggca atggctggcc cgccggctga ttttgagatg gcttttgtgc
     2341 aatcatcgcc ggcactggca cccgcgctgg atgtccgcgt cgtcgggttt tggcgctgga
     2401 agacggtgat tgtgccgccc aatgcctatg tggtgcatac tcgccggggc cactccgatc
     2461 cgttgcacat cgggttgggc atttcctttg ggttcaaccc ttattctgat tcatatctgg
     2521 ttgttccgtc ggcgatgcag acgattttga tcaacgccaa ctgtatctgt cgggaattac
     2581 aagggttgct ggtccagggc tatgtccagt ggattattgg cgactttgcc acggcttaca
     2641 aaaagctgga tttttccgat ctggttgacc ccatgcggat tgtcaacgtc cagttgcgcg
     2701 aacaggctga agcggccatc aaagacaaag tcgccaccat gagcattgac gatgtgctgt
     2761 cagacaagca gccaattatt caggaactga cggccagact tcggcaagtg gctgaagggg
     2821 aaggggatag cgataaagga ttggggctgc ggattgtcac ggttcaaatc aaagaagccg
     2881 tggtgagttc aacgcgtttg tgggaaaacc ttcaggaacc gtttcgtgcc gaccgtgccc
     2941 ggattgccaa tctggccaaa ctcgaagccg aatccgtaat caaggccgaa gaactcaaac
     3001 acgagcaaac gcgctctgtg accgaaatgg agagcaagag cgaactggcg cggattcgag
     3061 ccgccaaaga agctgaagtc tttgaccgcg aacaggccga aaaagcccgc cgccaccaga
     3121 aagaacagga agtcacccgg gcgacgctcc tggaacgcaa catcacgctc aagcaggagc
     3181 aaaaatccaa acgcgaactt gaagaagcca cgattgagaa ttcaatcgct ctggccaaag
     3241 ccaacgccaa actcgaacag gaacgactgg agcaggaatt tgcccgagaa cgcctgaagc
     3301 tcactgaaat gt
//
LOCUS       DAOQDO010000446         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22051_length_3312_cov_3.460547, whole genome shotgun sequence.
ACCESSION   DAOQDO010000446 DAOQDO010000000
VERSION     DAOQDO010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22051_length_3312_cov_3.460547"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..325)
                     /locus_tag="PKZ53_08410"
     CDS             complement(41..325)
                     /locus_tag="PKZ53_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40497.1"
                     /translation="MLLPDFVFRQASVGKKGLVSILPPFRHRAREQRAMTSHPEPVHC
                     GQKIPKRLPRLTAVAGLPHTSGSGTGIEWLSINRINNQDSGAASDIPWST"
     gene            867..2069
                     /locus_tag="PKZ53_08415"
     CDS             867..2069
                     /locus_tag="PKZ53_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HNJ40498.1"
                     /translation="MTVDQLKPDTMLSGRYRVVKRIGGGGMGNVYLVQDTRLNNRQRA
                     LKEMIGSFADDSSRQKAVQDFHREAELLASLEHPSIPTIYDYFDADGRYYLVMKFVPG
                     GDLEVKMRKNGGKIEERQVVEWAIQICDVLHYIHNQNPPIIYRDLKPANLMIDDTMQP
                     DRIVLVDFGIARFVAPTQKGVTAIGTMGYAPPELFAGKVEPRSDVYSLGATMFHLLTG
                     VNPQDNPLLIFDFNKNPRPCELNPALTPEIDSILCRSVEHKASKRFSAAEMKVALEEH
                     LKFLDSPRPAKTAMAPNATPRLVMLEPPMNVAFTLEKETHLIGRTDMYSGIFPEIDLT
                     PFEKETKVSRRHATIYRKGDQFYIEDLKSSNGTYVGNLRLTAHQPYLLQNGDELQFGG
                     TKLVFKAQ"
     gene            complement(2164..2838)
                     /locus_tag="PKZ53_08420"
     CDS             complement(2164..2838)
                     /locus_tag="PKZ53_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNJ40499.1"
                     /translation="MVKVKICGITSYQDAALALDYGADALGFIFNHKSPSYIAPSSAR
                     EIVEKLPPFVPLVGVFHNEFNLEAVTTIAQTAKVSILQLHGNESPGYCQQFSQWRLIK
                     TMRVGESFDFSDIRRFPVSAVLLEGYSEDNTTGKLFDWRIAAAAKQYARIILSGGLTP
                     ENVAAALRTVKPYGVDVSSGVEDSATPGRKDRMKIQMFMAEVERGRMEILKSSTGRLP
                     SLTGYS"
     gene            complement(3041..>3312)
                     /locus_tag="PKZ53_08425"
     CDS             complement(3041..>3312)
                     /locus_tag="PKZ53_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNJ40500.1"
                     /translation="TKGLVAVIEASDAMVKAANVTLIGYEKISAGFVTAIVRGDVAAV
                     KAATDAGAAAARRVGELISVHVIPRPHSSVNETLPVTHDSRPKRK"
BASE COUNT          808 a          856 c          875 g          773 t
ORIGIN      
        1 gttcacgctc aaggctcagg ttgggatttc aggttgggag tcacgtggac cagggaatat
       61 cggaggccgc acctgagtcc tggttattga tccggttgat gctcaaccac tctatgccgg
      121 tgccacttcc ggaggtatgt ggaagaccag caacggcggt caatcgtggc aaccgcttgg
      181 ggattttctg gccacaatgc actggctctg gatgcgaagt catcgcccgc tgctcgcgtg
      241 ctctatgccg gaacggggga aggatactga caagtccttt ctttccaaca ctggcctggc
      301 gaaacacaaa atccggaaga agcaaaagaa gtcctgccaa aaacgctcta tcagtatcgg
      361 gtacgggcag ttaccagtga tgacacttca gattcttcag ccgaaacgac cgtgccgccc
      421 cccagtggca cctccccaaa ttgcagagat tgcccccagg aaagccaaac cgggaaaact
      481 ggtgacgatc actgggaaca atttcgaaaa tggagtggtc ttttttgggc aacttcgggt
      541 aagcttagtg actgcttctg caacccggct tcaatttttg gttccacaaa ccccacgcgg
      601 agacgtgatt atcgcgatca cgaccccagg gggcagagcg caaataaact tcagggtaaa
      661 atagcagttc ctaaattggg cattgaagga actaggcgct cagcgtatat tccttcagca
      721 cacatcattc actattatcc agcgtgtcaa aattgagttc acttttgagg aatgcacagt
      781 cagagtcatt ctggggcagc ttttttcgtt agactgatgg actgtgatga gccatacacg
      841 cgctactgca ggggatttgg aaactaatga ctgtcgatca actgaaacca gacaccatgc
      901 tctctggccg ctatcgggtt gtcaaacgca ttggcggtgg cgggatgggg aatgtttatc
      961 tcgttcagga tacgcggctc aataaccggc aacgggccct caaggaaatg atcggttcgt
     1021 ttgcagacga ttcgagtcgg cagaaggcag tccaggattt ccatcgtgaa gcagaattgc
     1081 tggctagcct ggagcatccg tcgattccaa ccatttatga ttattttgac gctgacgggc
     1141 gctactacct ggtcatgaaa tttgttcctg gtggtgacct ggaagtcaaa atgcgcaaaa
     1201 acggcggcaa aatcgaagaa cgccaggtgg ttgagtgggc catccaaatc tgtgacgtct
     1261 tacactatat tcataatcaa aatccaccaa tcatttaccg tgatctgaag cctgcaaacc
     1321 tcatgattga tgacaccatg cagccggatc ggattgtcct ggttgacttt gggattgccc
     1381 ggtttgtggc gccaacccaa aaaggggtca cggccattgg aacgatgggg tatgccccgc
     1441 cggaattgtt tgccgggaaa gttgaacctc gttcggatgt gtattcgctt ggggcgacga
     1501 tgtttcacct gttgaccggt gtgaatccac aggacaaccc gcttttgatt tttgacttca
     1561 ataaaaaccc gcgtccgtgt gagttaaacc cggcgctgac acctgaaatt gatagcattt
     1621 tgtgccgttc ggttgaacat aaagcctcaa aacgcttttc agcggctgaa atgaaggtgg
     1681 cactcgaaga gcacttaaag tttttggaca gcccacgacc agccaagacc gctatggcac
     1741 caaacgctac gccacggctg gtgatgcttg agccacccat gaatgtcgcg tttacgctcg
     1801 aaaaagaaac ccacctgatc gggcgcactg atatgtattc cgggattttt ccagagattg
     1861 acctgacgcc gtttgagaaa gagaccaaag tctcacgccg ccacgcgacg atttatcgca
     1921 aaggcgatca gttctatatt gaagatttaa agagttcaaa cggaacgtat gtggggaatc
     1981 tgcggctgac ggctcatcaa ccctatctgc ttcaaaacgg cgacgagctt cagtttggcg
     2041 ggaccaaact ggtgtttaaa gctcagtaac tgacacggtg gcagggtgac agggtgacat
     2101 ttcttcgaaa accctgaacc ctgaaccctg aaccctgaac cctgaaccct gaaccctgag
     2161 ttcctacgaa tacccggtga gggacggcaa ccgcccggtt gacgatttca aaatttccat
     2221 ccgcccacgc tcgacttcag ccataaacat ttgaatcttc atccggtcct tgcgaccggg
     2281 tgtggctgag tcttcgaccc cggaagacac atccacgcca tagggcttga ctgtccgaag
     2341 ggccgccgcg acgttttcag gtgtcaagcc gccggacaga atgatgcggg catactgttt
     2401 ggctgccgcc gcaatacgcc agtcaaagag tttccccgtg gtgttgtctt cggaatagcc
     2461 ttcaagcagc actgcactga ccggaaagcg acgaatatct gaaaaatcga agctctcgcc
     2521 aacgcgcatg gttttaataa gtcgccactg gctaaactgc tggcaatagc cagggctttc
     2581 attgccatgg agttgcagga tgctgacctt ggcggtttgg gcaatcgtgg tcacggcttc
     2641 aagattgaat tcgttgtgaa agaccccaac cagtggaaca aagggcggga gtttttcaac
     2701 gatttcacgc gccgaggatg gggcaatata cgacgggctt ttgtggttaa agataaaccc
     2761 gagcgcatcc gcaccataat caagcgccag cgctgcatcc tgatatgaag taattccgca
     2821 gattttcacc ttgaccatag ggcctccggt tggtcggcag ggagtcgaac cagacaatgt
     2881 caggtagcag tccaatggcg agtcgcttga gaacaggatc tattcaacca gcacggaacc
     2941 ggcgtcaaat tcaccatctc gagttgcctc tggcaatttg ggttccctct ggctggttaa
     3001 agactcgctc cttgccactc gccattcgcg tttaagttgg ttatttccgc tttggacgac
     3061 tgtcatgagt aaccggcagt gtctcgttga cgcttgaatg tggacgtggg atcacgtgca
     3121 cgctgatcaa ttcaccaacc cggcgagcgg cagccgcacc cgcatcggtg gctgctttga
     3181 cggcggccac atcgccgcgc acaatggcgg tgacaaaccc ggcgctgatt ttttcatagc
     3241 cgatcaaggt gacattggct gctttgacca tggcgtctga tgcttcaatc acggctacca
     3301 ggccctttgt tt
//
LOCUS       DAOQDO010000447         3311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22068_length_3311_cov_3.129607, whole genome shotgun sequence.
ACCESSION   DAOQDO010000447 DAOQDO010000000
VERSION     DAOQDO010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22068_length_3311_cov_3.129607"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PKZ53_08430"
     CDS             <1..1062
                     /locus_tag="PKZ53_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNJ40501.1"
                     /translation="TGLQVHNGLAGLFLVSDDETSALKLPSGEFDIPLVIQDRVVDAN
                     NQFLYEGNGMMHGMDGFLGDRILVNGKPDYTLSVFTRAYRMRLLNGSNSRIYKLAWSN
                     GMPMTVIGTDGGLLETPIWRNYIMLAPGERVEIVVDFRKLSVGDTVKLLSLEFKGVEM
                     GMMSGNQLLENGAPFKVMSVVVERKEKQKKFAVPTKLSTIPRYRLEDAINRDRPRTFG
                     TSMQHMTWLLNGRPFEMNDVAENEIVQFGTMEVWNLVNDPGSRGMMGMSMAHPIHIHG
                     VQFQVLERQIATGFTTGWETVRAGYVDNGWKDTVMLMPGERVQLLLRFEDFPGLFVYH
                     CHNLEHEDRGMMRNFLIRS"
     gene            1104..2090
                     /locus_tag="PKZ53_08435"
     CDS             1104..2090
                     /locus_tag="PKZ53_08435"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNJ40502.1"
                     /translation="MNHQIQAVQSLCVSFFAIFLVFSAPSLVQGKPLLNGEAAMRVQD
                     HHYTCPMHADVTSDLPGDCNKCGMKLTPRSKDEKRIQAQISLETSPSVVMPGKPFVAR
                     FKVTDGTGTAIRRFEVVHEKLLHLMIVSRDLSYFDHIHPSLAGNVFSIKTKVPKAGNY
                     RMFADFVPTGGDQVIGKLDFSTSGKPTDPVPLVADTSMTKIFGTVEVTLTHSTPTLKA
                     GESLSLTFSLADAKTKKPITTLRPYLGAMGHCVILDQSATEYLHSHPEESGGTHHEHS
                     NGQAEHHHAPQGLGGPDVSFHTQFPKPGLYKIWGQFNYQGKILTAEYVVDVK"
     gene            2111..>3311
                     /locus_tag="PKZ53_08440"
     CDS             2111..>3311
                     /locus_tag="PKZ53_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ40503.1"
                     /translation="MLNKIIQWALNNRLLMVAGASLLLVYGTIVVINLPVDVLPDLNR
                     PTVTIMTEAPGLAPEEVETLVTFPIETLMNGATGVERVRSQSAVGLSVIFIEFGWSTD
                     IYIARQIVNEKLQLARSNLPANVTPVMGPISSIMGEIMLVGLKSKGNVTPPMDVRTLA
                     DWVIRPRLLTVPGVFQVIPIGGGRKQYQVLVDPAKLNQFNVTLQEVQEALVQSNSNTT
                     GGFLDKSGQEYLIRNLARFRSLEELAGTVVTYRQNTPVYVKNIGQVQYGPQVKRGEAS
                     TDAESAVILSIQKQPGSNTLQLTDQVEKALDEIQAGLPADIVIQKDLFRQATFISTAI
                     SNVKEALRDGAILVAIILFIFLLNFRTTFITLTAIPLSFIITFFVMKWFGLTINTMTL
                     GGLAVAIG"
BASE COUNT          868 a          730 c          814 g          899 t
ORIGIN      
        1 acggggcttc aagttcacaa cggattggct gggctcttcc tggtttcaga cgacgaaact
       61 tcggctctca aacttccttc cggtgagttt gacatcccgc tggtgattca ggaccgtgtt
      121 gttgatgcga ataatcagtt tttatacgag ggtaatggaa tgatgcatgg gatggatggc
      181 tttctcggcg accgcattct ggtcaatgga aaacccgatt ataccctgtc agtgttcact
      241 cgggcgtacc ggatgcggtt attgaatgga tccaattccc gcatctataa attggcctgg
      301 agtaatggta tgccaatgac cgtcattggt acggacggcg gattattgga aacaccaatt
      361 tggcgaaatt acattatgct ggcaccgggt gagcgggtcg aaatcgtagt tgatttccgc
      421 aaactttcag ttggagatac ggtaaagctt ctcagcctgg agttcaaagg ggttgagatg
      481 gggatgatga gtggaaatca actacttgag aatggtgctc catttaaagt gatgagcgtt
      541 gttgtcgagc gcaaagaaaa acagaaaaaa tttgctgtac caaccaaatt atccaccatt
      601 ccccggtacc gccttgagga tgccatcaat cgtgatcgcc cacgaacctt cgggacgtca
      661 atgcaacaca tgacctggtt actgaatgga cgaccttttg aaatgaacga tgtggctgag
      721 aatgaaatcg ttcaatttgg tacgatggag gtctggaatt tagtgaatga tcctggctca
      781 agaggaatga tgggaatgag catggctcat ccaattcata ttcatggtgt gcaatttcag
      841 gttcttgaac gccagattgc gactggattt acgactggct gggaaacagt gcgtgctggg
      901 tatgtagata acggctggaa agatacggtt atgctcatgc ctggcgagcg ggttcaattg
      961 ttgctcaggt tcgaagattt ccctgggctt tttgtatacc actgccacaa cctggaacat
     1021 gaagataggg gaatgatgcg gaatttcctg atccgttcct aacggaatgg catttttttc
     1081 aacaacacga atggaggtac gtcatgaacc atcaaattca ggcggttcaa agtctttgtg
     1141 tgtctttttt tgcaattttt cttgtttttt cagctccctc attggttcaa ggaaagcctt
     1201 tgctgaatgg tgaggcggcg atgagggttc aagatcatca ttatacctgc ccaatgcatg
     1261 ctgatgtaac ctctgattta ccaggcgatt gcaataagtg cggaatgaag cttaccccaa
     1321 ggtctaaaga cgaaaaacgt atccaggctc aaatttcgct tgaaacatct ccatctgtag
     1381 ttatgccagg taaaccgttt gtcgctagat tcaaggtaac agatggaact gggaccgcta
     1441 tccgaagatt tgaggttgtc catgaaaaac tcctccattt aatgattgtc agtcgggatt
     1501 tgagttactt cgatcacatc cacccatcac ttgctggaaa tgttttttct atcaagacca
     1561 aagttccaaa agctggaaat taccggatgt ttgctgattt tgttccgacg ggaggcgatc
     1621 aggtcattgg caaactggat ttttcgacca gcggcaagcc aacagatcca gttccactgg
     1681 ttgcggatac atccatgaca aagatatttg gtaccgttga agtaacctta acgcacagta
     1741 cgccgacact gaaagccgga gaaagtctgt ccttaacatt ttccctggct gatgccaaaa
     1801 cgaaaaaacc cattactacc ttgcgtcctt accttggcgc catgggacat tgcgtcattc
     1861 ttgaccaatc agccaccgaa tatcttcact cgcatccgga agagagcggc ggtacccatc
     1921 acgaacattc aaacggtcag gctgaacatc accatgctcc tcaaggattg ggaggtccgg
     1981 atgtcagttt ccacacacaa tttccaaaac cgggattgta caagatatgg ggtcagttca
     2041 actatcaggg taaaattctg actgctgagt atgtcgttga tgtgaagtaa gttattgaaa
     2101 gggatgtcga atgctgaata aaattattca atgggcactc aacaatcggt tgttgatggt
     2161 ggctggagcc tcactgcttc tcgtatatgg cacaattgtg gttataaatc tccctgtgga
     2221 tgtgttgcct gatctgaatc gtccaacagt caccattatg acggaggccc cagggttggc
     2281 tcctgaagaa gtggaaaccc tggtcacttt tcccattgaa acactgatga acggtgcgac
     2341 tggtgtcgag cgcgttcgat cacaatcagc ggttggactc tctgtaattt ttattgaatt
     2401 tggctggtcc acggacattt atattgctcg ccagatcgtt aatgaaaaac tccagcttgc
     2461 tcgatccaat ctcccagcga atgtgacacc tgttatggga cctatctcct caattatggg
     2521 cgagataatg ttggttggac ttaaaagcaa gggaaatgtc acgccaccga tggatgttcg
     2581 taccctggcg gattgggtaa ttcgtccacg ccttctgact gtgccaggtg ttttccaggt
     2641 cattcccatt gggggtgggc gcaaacaata tcaggtgctg gtggatccag ccaaattgaa
     2701 tcagtttaac gtcacactcc aggaagtcca ggaagcctta gtccaatcta acagtaacac
     2761 cacaggtggt ttcctggata aatcaggaca ggaatatttg attcgcaatt tggcccgctt
     2821 tcggtcgctg gaagaactgg ctggaacagt tgtgacgtat cgccaaaata ccccagtcta
     2881 tgtcaagaac attgggcaag tgcaatacgg cccacaggtt aaacgcggtg aggccagcac
     2941 tgatgctgaa tcagcagtga tcttaagtat tcaaaagcag cccggaagca acacactgca
     3001 attgacagac caggttgaaa aggcactgga tgaaattcaa gccggcctac ccgcagacat
     3061 tgtgattcaa aaagatttgt ttcggcaggc gacctttatc tcaaccgcca tttcaaatgt
     3121 gaaagaagct ttgcgcgatg gcgccatcct ggtggcgatc atcctgttta tttttcttct
     3181 gaattttcgc accactttta ttaccctgac agccattccc ttatcattta ttatcacatt
     3241 ttttgtcatg aaatggtttg ggttaacgat taacaccatg acgctcggcg gactggcggt
     3301 ggcaattggg g
//
LOCUS       DAOQDO010000448         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22085_length_3310_cov_2.705991, whole genome shotgun sequence.
ACCESSION   DAOQDO010000448 DAOQDO010000000
VERSION     DAOQDO010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22085_length_3310_cov_2.705991"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PKZ53_08445"
     CDS             complement(<1..1214)
                     /locus_tag="PKZ53_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ40504.1"
                     /translation="METQLIEVSTGAKLYVAKAGTGPELILFLHAVGGDHSSWMPQIE
                     AFQDRYTCISFDQRGHSRSEIPEMLLEHINVMTFAEDAAAVIAHFGFRRAHLVGLSMG
                     GVVALKVFRRFPHLVQSLTLANTWAFHPEAEARISFMQHQLEEMSLSKSSEMLIPGLF
                     APRTAQKIIDQAIRVEAAKDPDVFLASWQSMFGVDFRDLLSSINVPVLLLGGTEDKVT
                     PTDPLLTEIFARVPTSQLVNIDGAGHFSNLDHPAEFNRALGVFLGRSRSRSGAKAQFQ
                     ANPNITLEADTVAQGLMAMLSQRGVDCFFSNSGTDFTPIIDALARYQTSSEFKLKTIA
                     IPHENTAIAMAHGHYLVSGRPQVVMAHVNVGTANMGLGIINAARSRIPMLVLAGKTPW
                     YESDVEGCRTNFV"
     gene            complement(1273..3087)
                     /locus_tag="PKZ53_08450"
     CDS             complement(1273..3087)
                     /locus_tag="PKZ53_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ40505.1"
                     /translation="MFRSSRLKVLISACFLVGVGLVVALAIYQARAMRELKPDLTTVE
                     YAGSESCRQCHQSEFDHWSESHHHKMTQVASPQSVVGNFNNATYTFNGVTSRMFRQGD
                     EFFMETLDPGGQMATAKIVRTIGSRRFQQYVTQVGPTFWRLPIAWNIEKGYWFNLQGL
                     FLHADNPNFSQHTAIWNQNCIFCHNVKGNPGMDFDTRQFVNTNVKELGIGCEACHGPG
                     KLHIEKNQIPGWAWFRKKVRADDPTIVEPKKLEKTTELQVCGHCHGQRIPAQENAIRE
                     VMEKGDPYTAGENLSKYFTPLNITSKVGTYSFAQRFYPDGTPRLTAYEYQGVLMSKCY
                     QAGEMTCNSCHDVHKGRQQSLLTDDMRGNAGCVQCHQDINARLTEHTHHKADSNGSSC
                     NECHRPRIIFGLLTAKRTHRVLNPSPDKSLTTDMPNGCNMCHQKESTAWASKYLTEWY
                     GPEHALTAENQNHPEASKSEMVRELFSVDPSARCVAAWALGQPGANENGDAAKWAIPF
                     LIEALRDPYPAVRYFAYDSLKQLTQDESLKFFYLADSKDREAVIASFVAWWEKSPKPD
                     ARYRPTTVPLDTLWMIPATEQARYKASINKSLSIEIGE"
BASE COUNT          802 a          861 c          851 g          796 t
ORIGIN      
        1 acaaaattgg tgcggcatcc ctcaacgtct gattcgtacc agggcgtttt cccggccagg
       61 accagcattg gaatccgtga ccgggcggcg ttgatgattc ccagccccat atttgccgtg
      121 ccaacattga cgtgggccat cacgacctgt gggcgacctg acaccaggta atgcccgtgg
      181 gccatggcaa tcgcagtatt ttcgtgggga atggcaatcg tcttgagctt aaattcagaa
      241 ctggtctggt accgcgccag ggcatcaata atcggggtga aatccgtccc agaattggaa
      301 aagaaacaat ccacgccacg ttgcgaaagc atggccatca atccttgagc cacggtgtca
      361 gcttcaagcg tgatatttgg attggcctga aactgggctt tggcgccgct tcgacttcgg
      421 gaacgaccca aaaagacgcc cagcgcccga ttaaattccg ctgggtgatc aagattcgag
      481 aaatgcccgg cgccgtcaat gttcaccagt tgtgacgttg gaacccttgc aaagatttca
      541 gtcaggagcg ggtcagttgg ggtaaccttg tcttcggtgc caccaagcag caagaccggg
      601 acgttgatgg atgacagcaa atcacgaaaa tcaacgccga acatggattg ccacgaggcc
      661 aggaagacat ctggatcttt ggcggcttcg acccgaatcg cctggtcaat aatcttctgg
      721 gctgtccgtg gtgcaaataa ccctggaatg agcatttccg agctttttga aagactcatt
      781 tcttccaact gatgctgcat aaaggaaatc cgggcttcgg cttccggatg aaaggcccag
      841 gtattggcca gtgtcaggga ttgaaccagg tgtggaaacc ggcgaaagac tttcaacgcg
      901 accacgccac ccatcgaaag cccaaccagg tgcgcccgac ggaagccgaa atgggcaatc
      961 acggcggcgg cgtcttcagc aaaggtcatg acattgatgt gttccagaag catctccgga
     1021 atctcagacc ggctgtgccc gcgctgatca aaggaaatgc aggtataccg atcttggaac
     1081 gcctcgattt ggggcatcca ggacgaatga tctccgccca cagcatggag aaacaaaatc
     1141 agttccggtc ccgtgccagc tttggcaaca tacaatttgg cgccggttga gacttcaatc
     1201 agttgagttt ccacaaacac attttccttt ctcaaatgac aaggccgcct gatggcggaa
     1261 ctctggattt ttttattcac cgatttcaat tgataaactt ttgttaatgc tggctttgta
     1321 tctggcctgc tcagtcgctg gaatcatcca gagggtatcg agtggcacag tcgttgggcg
     1381 ataccgggca tctggtttgg gggatttttc ccaccaggcc acaaacgagg caatgacagc
     1441 ttcacgatct ttggaatcag ccagatagaa gaatttcagg ctttcatcct gggtcagttg
     1501 cttgagcgaa tcataggcaa aatatcgaac cgccgggtac ggatcgcgca atgcttcaat
     1561 caaaaacgga attgcccatt ttgcagcatc accgttttca ttcgcccctg gttggccaag
     1621 cgcccaggcc gccacacatc gggctgacgg gtcaacacta aacagttccc gaaccatctc
     1681 tgacttgctg gcttcagggt ggttttgatt ttcagcggtc agggcatgtt ctggaccata
     1741 ccactcagtc aggtatttcg aagcccaggc ggtgctttct ttctggtggc acatattgca
     1801 cccattgggc atatccgtcg tcaggctttt atcgggactg ggattcagca cgcgatgcgt
     1861 acgcttggca gtcaaaagcc caaagatgat ccgggggcga tgacattcat tgcacgaact
     1921 cccgttacta tctgctttat ggtgggtatg ttcagtgagt ctggcgttga tgtcctggtg
     1981 gcattgcaca cacccggcgt tgccccgcat gtcgtcagtg aggagcgatt gttgacggcc
     2041 cttgtggacg tcatgacaac tgttacaggt catttccccg gcctgataac acttgctcat
     2101 caaaacgccc tggtattcat aggcagtcag acgaggtgtg ccatctgggt aaaaccgctg
     2161 agcaaaactg taggtcccga ctttggaagt gatattgagt ggcgtgaaat attttgagag
     2221 gttttcacct gcggtgtaag gatcgccctt ttccatcacc tcgcgaatgg cattttcctg
     2281 agccggaatc cgctggccat ggcaatgccc gcacacttga agttccgtcg ttttctcaag
     2341 ttttttcggc tcaacaatgg ttggatcgtc agcgcgcacc tttttgcgaa accaggccca
     2401 gccggggatc tggtttttct caatgtgcag ttttccaggt ccgtggcacg cttcacagcc
     2461 aatgcccagc tctttgacat tggtgttgac aaactgccgc gtgtcaaagt ccattcccgg
     2521 atttcctttg acgttgtggc aaaaaataca gttctggttc caaatcgctg tgtgctggct
     2581 aaaattcgga ttatcagcat gcagaaataa tccctgtaag ttgaaccagt accccttttc
     2641 aatgttccag gcaatcggca accgccagaa ggtcgggcca acctgggtta catactgctg
     2701 gaaacgacgt gacccgatgg ttcgaacaat ctttgccgtt gccatctgac cacctggatc
     2761 aagcgtctcc ataaaaaact catccccctg ccgaaacatc cgcgatgtga cgccattgaa
     2821 ggtataggtg gcattgttga aatttccaac cacggattgc ggcgatgcca cctgagtcat
     2881 tttgtgatgg tgtgattcgc tccaatgatc aaattccgac tgatgacact gacggcagct
     2941 ttctgacccg gcatattcaa cggtagtcag gtcgggcttg agttcacgca tcgccctggc
     3001 ctgatagatt gccaaggcga caaccagacc aacaccaacc agaaagcaag cactgataag
     3061 gactttcaag cgagaactgc gaaacacaca acacctcaaa tcaatacaaa ttatgactgt
     3121 ttccaccgga ttttgtgttg gaaacaaatc agccgaaacc aacgagtcag taccacctgc
     3181 gtgagtgggt gggcctgtct gcttccaacc ccgcccgctc acgcaggtgg tactgacaag
     3241 atacccggca caatgggatt tgatggaaaa ttcagagaga tgggactaga ttgaaaaact
     3301 gatcttttgg
//
LOCUS       DAOQDO010000449         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22092_length_3309_cov_3.383221, whole genome shotgun sequence.
ACCESSION   DAOQDO010000449 DAOQDO010000000
VERSION     DAOQDO010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22092_length_3309_cov_3.383221"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..602
                     /gene="dtd"
                     /locus_tag="PKZ53_08455"
     CDS             153..602
                     /gene="dtd"
                     /locus_tag="PKZ53_08455"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNJ40506.1"
                     /translation="MRVVVQRVTRSAVVVNGVVVGRCGRGLNILLGVKIGDTQAEAEW
                     LANKVANLRIFEDDHGKMNRSLLEIQGEALVISQFTLYGDARKGRRPAFTDAEAPEKA
                     SKLVDYFVEVLQKMGLRVETGVFGAFMEVEIHNDGPVTILLEKDPEP"
     gene            635..976
                     /locus_tag="PKZ53_08460"
     CDS             635..976
                     /locus_tag="PKZ53_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNJ40507.1"
                     /translation="MSILLKTALLLALSNIFMTFAWYGHLKNLNHKKWYIAAFVSWGI
                     ALFEYLIQVPANRIGHTEFTVSQLKILQEVIALMVFAPFAIFYLGQPLKLDYLWAGLC
                     LLGAVYFIFRS"
     gene            1421..3169
                     /locus_tag="PKZ53_08465"
     CDS             1421..3169
                     /locus_tag="PKZ53_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNJ40508.1"
                     /translation="MVASIESLLRDPRMAGADQLVLQAGKHPIVMNSSGITDLTRTQL
                     SPFDIFKLLSPIIPEDKKAALMSQPTTQFQVNVSGAGPFDVTVSKEGGAMKVTFRPLG
                     GGSVGSAPPPPAPPPMSAPTPPRAPSGYPSPAPSGGVAYSPTSPSGGLAPPSAPSASS
                     FRPPQTPLPTPPQPPSHFSPPLQPRTPTPPGGFSSPPTSRPTGGSRRQGPLEMDKFFQ
                     KQLDLKASDLHLSTSAPPMLRLDGDMVVMEGFADSLTAEDTERMLWELMPDKNREEFS
                     RRNDTDFAYEYGTIARFRCNIFRDRKGMGGVFRVIPSKILTAETLGLSPEILKLCYLT
                     KGLVVVTGPTGSGKSTTLCAMVDYVNRNRSDHIITIEDPIEFTHENKKCLINQREVHN
                     HTSSFKDALRAALREDPDIVLVGEMRDLETIAIAIETAETGHLVFGTLHTSTAPSTVD
                     RIIDQFPADRQAQIRVMLSESLKGVIAQTLCKKKGGGRIAALEVLIIDSGVSNLIREG
                     KTFQIQSAMQTGKMKGNVLLNDALFDLVRRRLVEPQEAYMKAVDKGNFETMLKRANMD
                     LSFLNPSAQQGGNANS"
BASE COUNT          773 a          834 c          851 g          851 t
ORIGIN      
        1 gcacgcatcc tagcgcagtc attttgtgtc tgcaatctgc aaaacagagg gctgagggct
       61 tggggctgag ggcttggggg gtagaaatta agatattcga aaaacctcca atctcgaacc
      121 ctgaaccctg agccctgaac cctactcacc ttatgcgtgt tgtcgttcaa cgagttacca
      181 gatcggctgt tgtggttaat ggcgttgttg ttggtcgctg tggtcgcggt ctcaatattt
      241 tgctgggcgt aaaaattggc gatacccagg ccgaagctga atggctggcc aacaaagttg
      301 ccaatctgcg catttttgag gatgaccacg gaaaaatgaa ccgctctttg cttgaaattc
      361 agggagaggc cctcgttatt tcccagttca ctctctatgg cgatgcccgc aaaggtcgtc
      421 gcccggcgtt tactgatgct gaagcaccgg agaaagcttc gaagctggtt gattattttg
      481 tcgaagtcct tcaaaaaatg gggcttcggg ttgaaaccgg ggtctttgga gcgtttatgg
      541 aagttgaaat tcacaatgat ggcccagtca caatcctgct tgaaaaagac cctgaaccct
      601 gaaccctgaa ccctgaattt tagtgcagtg tgctatgtca attctgttga aaacggcgtt
      661 attgctggcg ctttccaata tcttcatgac ctttgcctgg tatggtcatc tcaaaaacct
      721 gaaccataaa aagtggtaca tcgcggcctt tgtgagttgg ggaattgccc tgtttgagta
      781 tttgattcag gttccagcca accggattgg ccacactgag tttacggtct cccagctcaa
      841 aattttgcag gaagtgattg cattgatggt ctttgctcca tttgcaatct tttatttagg
      901 gcaaccgctc aaactggact atttgtgggc cgggttgtgt ttacttgggg cggtgtactt
      961 catttttcgt tcgtgacggc atcaggtgga agacatcggg ctcaggactt ggggctgaag
     1021 acctcgggct gaaaacattg agttgaagaa ctggttttct ttcatccctc atctttcatc
     1081 cctcatccct tcgattgccc caagccccag gtcttaaatt gccggagcac acagaacaaa
     1141 cacgtgacgc cctctttaca tgctgtgcta agatgccagc ggctgtcact tcggttagtt
     1201 ttcattccct gaaaaagaaa atattcacaa acgctcacga ttcacttgcc gtggtgctta
     1261 gaggttgatg cggttttggt ttccacctgg atttctccaa tgggcttgac cagatgttgt
     1321 gatccaggaa ttggactggt attcaacctc aatctgcttc cccaaacagg taaatccgtg
     1381 catcctgcat cttgttatgg actttgaatg agggttggct atggttgcga gcattgaaag
     1441 ccttttgcgt gatccgcgca tggctggagc ggatcaatta gttttgcaag ccgggaaaca
     1501 tccaatcgtc atgaattcct ctggaattac tgacctgact cgaacgcaac tgagtccgtt
     1561 tgacattttt aagttgttat ccccgattat tccggaggat aaaaaagccg cgctgatgag
     1621 tcagccgacc acgcaattcc aggtcaatgt ctctggcgct ggcccgtttg atgtaacggt
     1681 ctcaaaagag ggcggggcca tgaaagtgac gttccgcccg ttaggtggcg gctctgtggg
     1741 cagtgcccca ccaccaccgg cgccgccgcc gatgtccgcg cctacgccac cacgcgctcc
     1801 atccgggtat ccgtctccgg caccttctgg tggcgtggct tactctccga cttccccgtc
     1861 gggggggtta gcgccaccct cagctccatc agcttcaagt tttcggcctc cacagacgcc
     1921 gttgccaact ccaccacaac cgccgtctca tttttcacca ccgcttcaac cacggacccc
     1981 cacaccgccg ggtggttttt cctccccccc aacttcgcgt ccgactggtg gctctcgtcg
     2041 ccagggaccg ttggaaatgg ataagttttt tcaaaagcaa ctggacctca aggcttcgga
     2101 cctgcacctg tccacttctg cgccgccaat gttgcggttg gatggggata tggttgtgat
     2161 ggaagggttt gcggattcac tcacggctga agataccgag cggatgttgt gggagttgat
     2221 gcctgacaag aatcgggaag aattcagccg ccgcaatgac actgactttg cctatgaata
     2281 tggcaccatt gcccgtttcc gctgcaacat ctttcgtgat cgaaaaggga tgggcggggt
     2341 attccgggtt attccaagca aaatcctgac tgccgaaacc ctgggattgt cgcctgaaat
     2401 tctcaagctg tgttatttga ccaaaggact ggtggtggta acagggccaa ctggaagcgg
     2461 aaagtcaacc accctgtgcg ccatggttga ctatgttaat cgcaaccgaa gcgaccatat
     2521 catcacgatt gaagacccga ttgaatttac ccacgaaaac aaaaaatgtc tgattaatca
     2581 gcgcgaagtc cacaaccata cgtcttcttt taaagacgcg ttgcgggctg cattgcgcga
     2641 agatccagat attgtcctcg tcggcgaaat gcgcgacctg gaaacgattg cgattgcgat
     2701 tgaaacggct gaaaccggcc accttgtgtt tggaacgctg catacgtcaa cggcaccgtc
     2761 caccgtggac cggatcattg accagtttcc cgctgaccgg caggcacaaa tccgggtgat
     2821 gctctctgaa tcactcaaag gagtcattgc acagacattg tgtaaaaaga aaggtggtgg
     2881 gcgtattgct gccctcgaag tcctgatcat tgattcaggg gtttccaatc tgattcgtga
     2941 agggaaaacc ttccagattc aatcagcgat gcaaactgga aagatgaaag gaaatgtgct
     3001 tctcaatgat gccctttttg atctggtgcg ccgtcggctt gttgaaccac aggaagctta
     3061 tatgaaggcg gttgataaag ggaatttcga aaccatgctc aaacgcgcca acatggacct
     3121 ttcattcctc aatccttccg ctcagcaggg tggcaatgcc aattcataag gttgaaaaat
     3181 cttcgggctg aagaagccgg gctgaagacc tggggctgaa gaagccgggc tgaagaagcc
     3241 gggctgaaaa cattgggctg agggttaaaa aaatgggatt tcaatcctca gcccgttttc
     3301 ttctgcccc
//
LOCUS       DAOQDO010000450         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22120_length_3307_cov_2.828106, whole genome shotgun sequence.
ACCESSION   DAOQDO010000450 DAOQDO010000000
VERSION     DAOQDO010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22120_length_3307_cov_2.828106"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2917)
                     /locus_tag="PKZ53_08470"
     CDS             complement(<1..2917)
                     /locus_tag="PKZ53_08470"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40509.1"
                     /translation="MIVLTVLCSVFVTPTQAHSGQESTPQPVQISSEKLPPGKPIEFE
                     VSGQTRQVFSFPLTANQFVKLELRVKTNGLTFKIIEPPAESATIQNDLESWSPLLPIL
                     ILAKTTGTYRLEITLDKRFETPSQYRVLLVETGEATGREIHQYEALKLLSLDANHPAQ
                     ESKEADLKTLEKYKEASHHYQLAGDFSSGSKILNSIGNGYYRFGQLAQARDYFEKSLE
                     SARKINEFDLIGQALTNIGLTYQVEGNGDKALELFLQALELRQKAQNRQGEGETLANI
                     AGIYRDRRQWDKALSIAFESLQCFRETHDQIAEAMMLDRIGLVHLNLDNFEPATEFFS
                     QALTIKRRLNVPSKIAETLSHLARVAAQQSEFQKALDLFEEAAQLIANTGQAQLQTSI
                     YSSLGRLYLNIGDFRRAESIIQQALELNRRIGNKRHQSSLRTNLGYVMAEQGRYLDAL
                     EQFTLALAIERDLKNPYGEGAVLHNLAFVLDRLGKQDEAITHLEEALVLRRQSRDKPG
                     ELATLQNLAEIFFTRGEKQKAIELAQHALDQWPKGSRPNDRALALSQLAYFRTENKEY
                     AQAKPLFEEALQLVETIREKVRSPELRTAFFSQNQFIYHNYINLLVRLHSQDPQAGYD
                     QMALQASERARARALLELLTESGAQIRRGADADLLKREQTIRQALAKKTEALIREQSQ
                     TAPSPKAEATLQTEIDSLTAAYQQLQEQLRQTSPHYAALTHPRPLTAREIQAKIVSSD
                     TALLEYALGPTRSFAWIVTPDSITTVELPKAAEIEQVARKAYKLLAQPADPNRDLKLA
                     PDHQNTTSSQELDQALSELSRLILAPVIPRLDRKRLLIVPDGVLHYIPFGALTVRASG
                     SKAAPLLTHFELVTLPSASALDVLRTQTPNHLPSSKNLLVVADPVFDASDNRLLNRAT
                     DLPSPQRVEEKTDRSRKLRLENAKASVQAVGISGESAHIPRLPGTRRARRTRGARYRS
                     "
     gene            complement(3085..>3307)
                     /locus_tag="PKZ53_08475"
     CDS             complement(3085..>3307)
                     /locus_tag="PKZ53_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015783728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ40510.1"
                     /translation="GFMYAGSPRVVVSLWNVSDQATAELMKHFYQGLLVEKLRPADAL
                     RQAQLRLRQHKKWSSPYYWAAFQLTGEWK"
BASE COUNT          733 a          762 c          936 g          876 t
ORIGIN      
        1 gcgagcgata tcgcgcgccg cgcgtccttc gcgcacggcg cgtgcctgga agtcgtggaa
       61 tgtgggctga ctcaccgctg attccgaccg cttgaactga agctttggcg ttttcaagcc
      121 gcagtttacg actgcggtca gttttctctt caactcgttg aggggaagga agatcggttg
      181 cccggttgag aagccgattg tcactggcgt caaataccgg atccgccacg acaagcaggt
      241 ttttggaaga gggaaggtga ttgggggtct gggtacggag cacatcaagg gctgatgctg
      301 aaggcagtgt gaccagctca aaatgagtta aaagtggtgc tgcctttgaa cctgaagcgc
      361 gtaccgtcag tgccccaaat ggaatgtaat gcaggactcc gtctggaacg atcagcaatc
      421 gtttccggtc taatcgtggg atgaccggcg ccaagatcaa acggctgagt tctgaaaggg
      481 cctggtcaag ttcctgactt gaggtggtgt tctggtgatc aggagccagt ttgagatccc
      541 ggtttgggtc agccggttga gccagcagct tgtatgcttt tcgggcaacc tgttcaattt
      601 ccgctgcttt ggggagttcc actgtggtta tcgaatctgg cgtcacgatc caggcaaagc
      661 tccgagttgg gcccaaggca tattccagca acgcggtatc agatgaaaca attttggctt
      721 gtatttcacg ggccgtaagt ggtctgggat gagtcaatgc tgcatagtgt ggactggtct
      781 gacggagctg ttcctgaagt tgctgatacg ctgctgtcaa agaatcaatc tcagtttgga
      841 gtgtggcctc ggctttgggt gagggcgctg tttgtgattg ctcgcggatc agggcttcag
      901 tttttttggc gagtgcctga cgaatggttt gttcgcgttt gagcaggtca gcatccgcgc
      961 cccggcgaat ttgagccccg gactcagtaa gcaattccag gagagcacgg gcacgggcgc
     1021 gttcactggc ctgaagggcc atctggtcat atccggcttg tgggtcctgg gaatgcagcc
     1081 ggaccagaag attgatgtaa ttgtggtaaa taaactggtt ttgtgaaaaa aacgccgttc
     1141 ggagttcagg cgatctcact ttttcacgaa tggtttcaac cagttggagg gcttcttcaa
     1201 agagcggttt ggcctgggca tattctttat tttcggtccg gaagtaggca agctggctca
     1261 gggccagggc gcgatcattc gggcgactgc cctttggcca ttggtccagg gcgtgttggg
     1321 cgagctcaat ggccttttgt ttttcccccc gggtaaagaa aatttcagcc aaattttgaa
     1381 gggtcgctaa ttcaccaggc ttgtcacggc tttggcgccg aaggaccagg gcttcttcca
     1441 gatgagtgat tgcctcatcc tgttttccaa gtcgatccag cacaaacgca agattgtgga
     1501 gcacggcgcc ttcgccatac ggattcttga ggtctctctc aatggccaaa gccagggtaa
     1561 attgttcgag ggcgtccaga taccgtcctt gttcggccat gacgtaccca aggttggtcc
     1621 gcaatgagct ttggtgtcgt ttatttccaa tccggcgatt gagttccagt gcctgttgga
     1681 taattgattc agctcgccga aaatccccga tgttgagata gagccgtcca agacttgaat
     1741 aaatagatgt ctgtagctga gcctgaccgg tgttggcgat caactgtgcg gcttcttcaa
     1801 ataaatcgag ggctttttga aattcacttt gttgggcagc tactctggct aagtgggaca
     1861 gggtttcagc gattttggat ggcacgttca accgtcgctt gattgtgagt gcctgggaaa
     1921 agaattcggt tgccggttca aaattgtcca aattgaggtg gaccaggcca attcggtcca
     1981 gcatcattgc ttccgcaatt tgatcgtgag tctctcgaaa acattgaagc gactcaaacg
     2041 cgatgctgag tgccttgtcc cattgccgcc tatcccgata gatgccggcg atattggcca
     2101 acgtttcgcc ttcgccttgc cgattctgag ccttttgccg gagttccagg gcttgcagaa
     2161 acagttccag ggctttgtcg ccattcccct cgacctgata ggttaatcca atgttggtca
     2221 gtgcctggcc gatcaagtcg aactcattga ttttccgggc tgattcaagg gatttctcaa
     2281 agtagtcgcg ggcttgagcc agttgaccga atctatagta cccattgccg atactgttta
     2341 agattttaga tccagagcta aaatcacccg caagctgata atggtgactg gcttctttat
     2401 atttttcaag tgttttcaag tctgcttctt ttgattcctg ggccggatgg ttggcgtcaa
     2461 gactcaacag tttcaacgcc tcatattggt gaatttcacg tccggtggct tccccggttt
     2521 cgaccagaag cacccgatac tgagatggtg tctcaaaccg tttatccagt gtgatttcaa
     2581 gccgataggt gccggtcgtt ttggccagaa tgagaattgg aagcagaggc gaccacgact
     2641 ccaggtcatt ttgaatcgtc gcagactcgg ctggcggttc aatgattttg aaggtgagtc
     2701 cgttggtttt gacacggagt tcgagtttga caaattgatt ggcggtcaac ggaaaggaaa
     2761 atacctgccg ggtctggcct gaaacctcaa attcaattgg ttttccaggc gggagttttt
     2821 cagacgagat ctgaacgggt tgaggcgtgg attcctgacc tgagtgagcc tgggttggcg
     2881 tgacaaaaac agaacaaaga actgtcagga caatcatgtg aaaagaccag ttcttgcctg
     2941 cttgctttgg aaattgttgg aaaaagaagt acataacagg tagtgaaaac agtgcgcggt
     3001 aaaatagcgc tggttgtatg aagcaaaaac cgttgggctt aaatgaaaat cgcgcgtgac
     3061 ttaccagttt tttaccctga cctattattt ccactcgccg gtcagttgaa acgccgccca
     3121 gtagtaaggc gaagaccatt ttttatgctg ccgcagcctg agttgtgcct ggcgaagtgc
     3181 atcagccgga cggagttttt cgaccaggag tccctgataa aagtgtttca tgagttcggc
     3241 tgtggcctgg tcgctgacat tccacaagct gaccacgact cgcggagaac cggcatacat
     3301 aaaccca
//
LOCUS       DAOQDO010000451         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22153_length_3304_cov_2.305940, whole genome shotgun sequence.
ACCESSION   DAOQDO010000451 DAOQDO010000000
VERSION     DAOQDO010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22153_length_3304_cov_2.305940"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PKZ53_08480"
     CDS             complement(<1..1035)
                     /locus_tag="PKZ53_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YD repeat-containing protein"
                     /protein_id="HNJ40511.1"
                     /translation="MLRRSLKAIFLFTLLLLGLNSAQGQQSPTDTGTPAALLKGAHPL
                     GSYSGSSFDQVNLFNGNASFSIPLANLNGRAGLGVGVMLSYNSKVWRTESVPGDSTFH
                     SPTYISWDETSPLLAAGWSLSGGHLVARQVNYSEPASCIEGQLGQYTPRLQYTILKLT
                     FTAPDGTEFDFRDDLTDGQPLPLYDRCTLVGPGSRGQRFHSADGSGATFISDNVVYDR
                     LVEYSEPLGFPTSPFPVSGVVILRDGTRYTVNGQGNVVKREDRNGNIVRFDYDASGRL
                     ITLTDTLERTITITYGDPGASRIATISWQGFGGVTRTTTIDTKKLRNALRPGEQISRY
                     STLFPTLRPPS"
     gene            complement(1117..3111)
                     /locus_tag="PKZ53_08485"
     CDS             complement(1117..3111)
                     /locus_tag="PKZ53_08485"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNJ40512.1"
                     /translation="MKHRHALLIFFVLIGCGFLFRTYLPLHPTLAKADPPGVVTRRAP
                     QINGRVEGSVQMLTGENITLNGGSTITGDLLVPGTPTVRRNGNPNLGGVISGNGNPQP
                     TNYQVTLNGNATLGRLVIRTDPFTLSPVAAPPLPVGTRDVTLNNPNDQIGDATTLRNL
                     TLNGNAGTRSVPPGTYGTFTVNGNSALVFGVAGNTQPSLYNLQGLTLNGGAQLQVIGP
                     VILVLRNGTNVNGILGSSSTPLWLSLKIASGGLTVNGGGQVYGSVAAPSGTITLNSRL
                     VGNAACDRLVVNGGGVLQVVMPSTAEIVIQPSTLPDGRVEGNYDQMLTASGGTSPYTF
                     SLTSGGLPTGLTLDSSGRITGVPLQEGSFPLTIMAADANGNTGSQAYTLVIKPACPII
                     AITPATLADGTIGVAYSQALTAQGGVGNITFSMGSGTLPAGLALAASGVISGTPTASG
                     TFGFGVNATDTQGCTTTQPYQIVIGGGCQLAIGLTSLPNGKVNSPYSQTLTATGGTGT
                     VTFTLSSGSLPAGLNLIETGNVSGIPTASGSSTFSVTATDSQGCGANATYTLVVDPLI
                     APGTQFLTFNVSPTDFDPDQISISAGKANFYLRNYTGIATSFELVSPTGAITSLPGLM
                     GRNLSPELTLTPGEYTLRDAGNPQVSCRITVTAARKSGRK"
BASE COUNT          844 a          827 c          856 g          777 t
ORIGIN      
        1 tgaggggggc cgcaaagtag gaaataacgt gctgtaccgg gaaatttgct cgccaggccg
       61 gagcgcattt cgcagctttt tggtatcaat tgtggtcgtg cgggtgactc cgccaaatcc
      121 ttgccagcta atggtggcga tccgagaggc tcccggatca ccataggtaa tggtgatggt
      181 tcgttcgagt gtatcagtca gcgtgatcaa ccgaccgctg gcatcatagt caaaccggac
      241 aatattgccg tttcggtctt ctcgtttgac cacatttcct tgcccattga ctgtataccg
      301 tgttccatcc cgcagaataa caacaccgga aacggggaac ggagaagttg gaaacccaag
      361 tggttcacta tactcaacca gccgatcata caccacgttg tcactgataa aggtggcacc
      421 tgaaccatct gcactgtgga accgctgtcc acgagaacct ggaccaacca gagtacaccg
      481 atcatagagt ggtaagggct gcccatcggt gagatcatcg cgaaaatcaa actctgtccc
      541 gtctggcgcg gtaaatgtca gtttcagaat ggtatattgc aaacgtgggg tatattgccc
      601 aagctgtcct tcaatacagg aagcgggctc cgaataattc acttgccgtg cgaccagatg
      661 cccacccgaa agtgaccagc ctgccgccag taggggtgat gtttcatccc aactgatata
      721 ggttggtgaa tgaaatgtgg aatccccagg tactgattcg gtacgccaga cttttgagtt
      781 gtaggacagc atcacgccga ccccaaggcc agcccgacca ttcagattcg cgagcgggat
      841 gctgaatgaa gcatttccat taaacaggtt cacctgatca aacgaacttc cgctgtaact
      901 tccaagggga tgagcacctt tgagcaatgc cgccggtgtg ccggtgtcag ttggactttg
      961 ctgtccctga gcactgttca aacccaataa aagcaatgta aacaggaaga tagctttcaa
     1021 gctgcgtcgc aacataattc tttgtatcct tcaattcttt tcagcgatgt tcattcgttc
     1081 ggtcaaccca aaaattatca gtgggtaggc aggtgattac tttcgacccg atttcctggc
     1141 agcagtaacc gtaatccggc aacttacctg gggattaccc gcgtcccgaa gggtgtattc
     1201 acctggggtc aatgtcagtt caggtgacag attgcgtccc atcagaccgg ggagcgacgt
     1261 aatcgctccg gttggggaaa ccagctcaaa actggtggct atcccggtat agttgcggag
     1321 gtagaaattg gctttaccgg ctgaaatgga aatttgatcc gggtcaaaat cagtcggcga
     1381 gacattaaaa gtaagaaatt gagttccagg agcaatcaac ggatccacca ccagagtata
     1441 ggtggcattt gccccgcatc cctggctatc agtggcggtg actgaaaaag ttgaactccc
     1501 tgaagccgtt gggataccgc tcacattacc ggtctcaatc agattcaaac cagcgggaag
     1561 cgatcctgaa gacagtgtaa atgtcaccgt cccagtacca ccagttgcgg tcaatgtttg
     1621 ggagtacgga ctgttgactt ttccatttgg caatgaagtc aacccaatgg caagctggca
     1681 accaccacca atcactattt gatacggctg ggtcgtggta cagccctggg tatcggttgc
     1741 atttacccca aagccaaagg ttccggaagc tgtcggggta cctgatatca ccccactggc
     1801 agccaatgcc aatccagcag gtaaggtgcc cgatcccatc gaaaaggtaa tattgccgac
     1861 tccaccttga gctgttaacg cctgggaata cgctacacca atcgttccat cagctagtgt
     1921 ggcaggggta atggcaatga tcggacaggc tggtttgata acgagcgtgt acgcctggct
     1981 cccagtattc ccattggcat ccgcagccat aattgtcaga gggaaagacc cttcctggag
     2041 tgggactccg gttattctcc cactcgaatc aagcgtcaac ccggtcggta atccaccact
     2101 ggtcaagcta aaggtataag ggctggtgcc gccacttgcg gtcaacatct ggtcataatt
     2161 cccctcaaca cggccatcag gaagggttga cggttgaatt acaatttcag ccgttgatgg
     2221 catcacaacc tgaagcacac cgcctccgtt gacaaccaga cgatcacagg cagcattccc
     2281 aaccagacga ctattcaggg tgatggttcc actcggagcc gcgaccgagc catatacctg
     2341 gcctccgcca ttcacagtca agccgccact ggcaatcttg aggcttaacc acaatggagt
     2401 ggaggagctt cccaaaattc cgttgacatt tgttccattg cggagaacca gtatcaccgg
     2461 cccaatgact tgcaactgtg cgccaccgtt gagggttaaa ccttgtaaat tataaagaga
     2521 aggttgggta ttccctgcca ctccaaagac caatgcactg ttgccgttga cggtaaatgt
     2581 gccgtatgtg ccaggtggca ccgaccttgt tccggcattc ccatttaagg tcaggttgcg
     2641 aagggttgtg gcatctccaa tttggtcatt tggattgttt aatgtcacat cccgtgttcc
     2701 gactggaagc ggcggggcag caaccggtga caaagtaaac ggatctgttc ggattaccaa
     2761 tcgcccaaga gttgcattgc cgttgagtgt aacctggtaa ttggttggtt ggggattgcc
     2821 attcccagag atgaccccac ctagatttgg atttccattt cgcctgacgg ttggggtacc
     2881 tggtaccaac agatctccag tgatggttga tccaccattg agggtgatgt tttcaccggt
     2941 caacatctgg actgaaccct ccacgcggcc attgatttga ggtgcgcggc gggtaaccac
     3001 gcctggcggg tcggctttgg caagggttgg atgaagcggg agataggttc ggaaaaggaa
     3061 tccacatccg atcaaaacaa agaaaatcaa aagagcgtgc cggtgtttca ttttgtagtt
     3121 gctccaaaaa ggcggagaag gtggtcggta ttttttaagc gacgtaaact ggttatttgc
     3181 acggagaggt aaaaatgatt cagagaaaag accggaaaaa ccagaggaat agtttttcca
     3241 gtcaaaataa attacccatg ttgaattgag tctgaatcat ccaggtcaat cactgtgcca
     3301 ggga
//
LOCUS       DAOQDO010000452         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22202_length_3299_cov_3.406905, whole genome shotgun sequence.
ACCESSION   DAOQDO010000452 DAOQDO010000000
VERSION     DAOQDO010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22202_length_3299_cov_3.406905"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKZ53_08490"
     CDS             complement(<1..229)
                     /locus_tag="PKZ53_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ40513.1"
                     /translation="MKSIHLKPLNDKVLVHTETNLLESLLAQQCEIAMACGGQGICAT
                     CHVYVKEGKDALSPPTVREQRTLSLITSSNNN"
     gene            510..707
                     /locus_tag="PKZ53_08495"
     CDS             510..707
                     /locus_tag="PKZ53_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40514.1"
                     /translation="MKAIVERSGKQAPQARTPGNSKSFFSCNITPSLMKLFYIRALTV
                     FARNNQTSSADEQGNLVVRVL"
     gene            complement(794..2068)
                     /locus_tag="PKZ53_08500"
     CDS             complement(794..2068)
                     /locus_tag="PKZ53_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ40515.1"
                     /translation="MPTHQVPHPSILFVDDEASFLETVGDFFTLKSNGEIKIKLAQNV
                     AQAIKIIEQEPVKVIIIDIHMPVVDGLQFMTLLHHKHPRIRKIILTGYATDTYRTTAL
                     KSGASLFLEKPRSIDEFESLYETITELMRQTTTDRGFSGLVHHTNLSDMLQMFCLSGS
                     SLVLEVQSELCDGGIFIKNGTIVHVQSNDRIGFEAFFEIMSLMGGEFTVKPFLEPPTI
                     SITDSWEHLLLETARLTDEGKPPDQYEPPVLEPFAQVIPDLEASLASQSLPVTDSPHR
                     LVNSEPNQVGSEVDDFPQDHRFADISVSPSSKPSETLEAPIISRWSPLSADWCTELVL
                     TNPQSEVIEAHSSEDPNLRADLLDFLSMKTSQISQALNARELHHLELQGVTFSAVVLI
                     NPEIKVFALSTKPEVDCQGIIDKLPPVFPSQL"
     gene            complement(2367..>3299)
                     /locus_tag="PKZ53_08505"
     CDS             complement(2367..>3299)
                     /locus_tag="PKZ53_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNJ40516.1"
                     /translation="GSIRQPASLCGIVGLKPTYGRVSRYGLVAFGSSLDQIGPMAKTT
                     RDVARMLGVLAGRDTHDATSAQVEVPDYLAELTGNIKGLRIGIPKEAFGDGLDPEVNA
                     VIQQALTRYQELGAELVETSLPNSGYAIPDYYIIATAEASANLARFDGVRYGFRVEGV
                     KTLHEMYQKTRDLGFGPEVKRRIMLGTYTLSSGYYDAYYLKAQKVRTLLRQDFERAFE
                     QCDVLLTPTSPTPAFKLGSKTDDPLQMYLSDIYTVTLNMVGVPGLSLPCGLSEAGLPI
                     GMQLIAPHFAESRLLNTAFAYEQAYPFPKSPALA"
BASE COUNT          854 a          767 c          854 g          824 t
ORIGIN      
        1 aattgttatt gcttgaagtg atcaggctga gtgtccgctg ctctcgaacc gtcggtgggc
       61 tgagggcgtc tttgccttct ttgacataga catggcaggt cgcacaaatt ccctgaccac
      121 cacaggccat ggcaatttca cactgttgag ccagcagtga ttcgagcagg ttggtttcgg
      181 tatggaccaa aactttgtcg ttcagcggct tcagatggat agatttcatg aatagtgggt
      241 gctcctcagt cagaactggt tatgacgcga cagtcagaga ttcagtcaga tactgcttta
      301 tcagattttg atctgactga gaaagatttc tttccgtcaa aatgccgaga acaacaccat
      361 ccggggctga catgcaaaaa atagcctggt cctgaaacat ccatctaaga tcgtgagctg
      421 gcaggcgatg caggtgtagc agcttgaagg tatcattggc tgatcgccag attccttcaa
      481 ggaccgatat ggaaagggta gaatcccgtg tgaaggcaat cgttgagcga tctggaaaac
      541 aagctccgca ggcccgaacg ccaggaaact ccaagagctt tttcagttgc aacatcacgc
      601 cttctctcat gaaattgttt tatatccgtg ccttgaccgt atttgcccgc aacaaccaga
      661 catccagtgc tgacgagcag ggaaatttag tggttagagt gttgtaattt tgttgcaatt
      721 gtgcgattgc accggaatca ttccggggac caatccaact atggaagatc aaatctgcgg
      781 cgtgagtatg tgattacagt tgtgaaggga atacaggagg gagcttgtca atgatgccct
      841 gacagtctac ctcgggtttc gttgagagtg caaatacttt tatttctggg ttgatcagca
      901 ctacggctga aaacgtaacc ccttgtaatt caaggtgatg aagttctctg gcattgagtg
      961 cctgactgat ttggctcgtt ttcatcgaga gaaaatccag caaatcggcg cgtaagtttg
     1021 ggtcctctga actatgggcc tcaatcacct cactttgggg gttagtcagg acaagttcag
     1081 tgcaccagtc tgctgataac ggtgaccatc tggatattat gggagcttcg agtgtttctg
     1141 agggtttgga tgagggagat actgaaatat ctgcaaatct atgatcctgg gggaaatcat
     1201 ccacttctga tcctacctga tttggttctg aattgacaag tcggtgagga gaatcggtga
     1261 ctgggagcga ttgtgaggcc agggaggctt cgagatcagg gattacctgg gcaaatggtt
     1321 caagcacggg gggctcgtac tgatcagggg gctttccttc atcggtcaat cgggcggttt
     1381 caagcagcag atgctcccag gaatcagtaa ttgaaatcgt cggtggctca agaaacggct
     1441 tgaccgtaaa ttcaccaccc atcagtgaca taatctcaaa aaacgcctca aatccgatgc
     1501 ggtcatttga ctggacatgc acaatggtac cgtttttgat aaagataccg ccatcacaca
     1561 attcggattg tacttccagc accagcgaac tcccactcaa acaaaacatc tgcaacatat
     1621 cactgaggtt ggtgtgatgc accagccctg agaacccacg atccgtggtt gtctgccgca
     1681 ttaactcagt gatggtttca tagagactct caaattcatc aatggagcgt ggtttttcca
     1741 aaaataacga agcaccagat tttaatgcgg ttgtccggta ggtatctgtc gcatacccgg
     1801 tcaggataat ttttcggatt ctcggatgtt tgtggtgcag caaggtcata aactgcaacc
     1861 catcaacgac tggcatgtga atgtcaataa taatgacttt taccggctct tgttcaatga
     1921 ttttaattgc ttgagctaca ttttgagcca gtttgatttt tatctctccg ttacttttga
     1981 gtgtaaaaaa atcaccaact gtctctaaaa acgaggcttc atcatctaca aacaaaatcg
     2041 aaggatgtgg cacttgatga gtgggcactt gggaaaccgc gggcataaac aaatgtacca
     2101 aaaccttgat ccaacttaag ctgaaacagg tccgatttgt tgatttgtca ggtgataaag
     2161 atttgagcga caccctaacc caatcactca cattcaacgt ggtatgagac aaccagtgaa
     2221 ccagtttgtt tgagaggcaa catgcaagat tgtatgacca ggcggcagca tacaatggtg
     2281 aaacagcgac tgcaatcgaa aatggagagg ggggagagga accgtcatct gctcctctat
     2341 ggaggatgac ggttcctaat tcagcattat gccagggcgg gtgattttgg aaatgggtag
     2401 gcctgttcat aagcgaaagc tgtgttgagc aaccggcttt cggcaaaatg cggggcaatc
     2461 aactgcatgc cgattggcag acccgcttca ctgagtccgc agggcaggct caagccagga
     2521 acacccacca tattgagcgt gacggtgtag atatctgaaa gatacatctg caacggatca
     2581 tcggtttttg aaccaagttt gaaggctggt gtcggcgagg tcggtgtcaa caggacatca
     2641 cactgctcaa acgcacgttc aaaatcctgg cgcaacagcg ttcggacttt ctgggctttg
     2701 aggtaatagg cgtcatagta gccggaggac agcgtgtagg tgccgagcat aatccgccgt
     2761 ttgacctcag ggccaaaccc aagatcacgc gttttctgat acatctcgtg cagggttttc
     2821 acgccttcaa cgcggaatcc atagcggacg ccatcaaacc gggccaggtt ggcgctcgct
     2881 tcggcggtgg cgatgatgta gtaatcggga atcgcatagc ctgaattggg gagtgacgtc
     2941 tcgaccagtt cagcacctaa ttcctgatag cgggtgagtg cctgctgaat caccgcattg
     3001 acctctgggt ccagcccatc gccaaaggct tctttcggaa tgccaatccg caaccccttg
     3061 atattgccgg tcaactcagc cagataatcc gggacttcaa cctgagccga tgtggcatcg
     3121 tgggtatcgc gaccagccag gacgccgagc atccgggcca catcgcgggt ggtttttgcc
     3181 atgggcccaa tctgatccag cgatgatcca aaggccacca gtccatagcg cgacacccgg
     3241 ccataggttg gttttaatcc aacaatgccg cacaaactgg ctggctgacg gattgaccc
//
LOCUS       DAOQDO010000453         3297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22234_length_3297_cov_2.657002, whole genome shotgun sequence.
ACCESSION   DAOQDO010000453 DAOQDO010000000
VERSION     DAOQDO010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22234_length_3297_cov_2.657002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKZ53_08510"
     CDS             <1..1286
                     /locus_tag="PKZ53_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ40517.1"
                     /translation="LAIAEDHVNPNLLFLGTEFALYFTPNGGQKWIQLKGGLPTIAIR
                     DLAIQKRENDLVVATFGRGFYVLDDYSALRNISAEALNGPECLLYPTRETLLYIQSRP
                     IGESGKGSQGANYFTTENPPFGATFTYYLKDELQTKKQKRQEAEKEAVKKKTTAPYPT
                     PDQLRAEAEEEPPTIILTVTDASGRVVRKLTGKNQSGFNRVTWDLREPEPELPSETQP
                     DFVDEDGGTFGALVMPGRYKVSIAKRVDGVLTQLAGPQEFSVVAEGTDTMPQADLAAL
                     TEFQQKAMKLKRVVSGALEVAGDTKDRLKKIKQAIHETPQADNKLMDDALSIEKRLNA
                     ILIALRGDVVLRARNENTLTSISERIDNILSEQRMSTSRPTQSHIDSLKIADEEFRVE
                     LEKLRTLLEVDLKNLDKALETAGAPWTPGRLPEWR"
     gene            1388..2053
                     /gene="modB"
                     /locus_tag="PKZ53_08515"
     CDS             1388..2053
                     /gene="modB"
                     /locus_tag="PKZ53_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNJ40518.1"
                     /translation="MSFFHPLFLSFEVALAASTLALAVGATFAWILAKLRFPGKEWCD
                     VLLTLPLVLPPTVLGYYLLVVIGNRSVIGRAWESCFGNPLVFTPTAAVLAAFMSALPL
                     VVKSARSALEAVETEAENAARLLGASEWRVARTITLPLAWRGLAAAGTLAFARALGDF
                     GATLMVAGNLPGKTQTAALAIYDAVAAGRDSEALMLSGVMSVVAAVLLYGVNRLTAAH
                     HKW"
     assembly_gap    2270..2369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2426..3037)
                     /locus_tag="PKZ53_08520"
     CDS             complement(2426..3037)
                     /locus_tag="PKZ53_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNJ40519.1"
                     /translation="MLVPMVVEQTARGERAFDIYSRLLKENIIFINGEIDDDMANLVV
                     AQILFLEAEDPEKDINIYINSPGGSITSGLAIYDTMQFVKPDIVTLGIGMCASMASVL
                     LAAGTKGKRCALPNTCVMIHQPHMGGRGMRGQATDIKIYANEIIRLREIISGLLSSHS
                     GKPFDLVEQDVERDRYLTAPQALEYGLIDTILDRRDVVGEEGK"
BASE COUNT          799 a          863 c          802 g          733 t
ORIGIN      
        1 ccctggccat tgccgaagac cacgtcaatc cgaacttgct ctttctgggc actgagtttg
       61 cgctctattt cacacccaac ggcggtcaga aatggattca gttaaaaggt ggcctgccaa
      121 cgattgccat tcgtgatctg gccattcaaa aacgcgaaaa cgatctggtg gtggcgacct
      181 ttggacgtgg attttatgtt ctggatgatt attcagctct gcggaatatc tcagccgaag
      241 ctctcaatgg accagagtgt ctgctttacc caacccgtga aaccttgctc tatatccagt
      301 cgcgtccgat tggggaaagc gggaaaggct cgcaaggggc caattacttc acgacggaaa
      361 acccaccctt tggtgcaacc tttacttact atctgaaaga cgaactccag accaaaaaac
      421 aaaagcgcca ggaagctgaa aaagaagccg tgaaaaagaa aaccacggcg ccatatccaa
      481 cgccagatca attacgtgcc gaagccgaag aagaaccacc gaccatcatt ttgacggtca
      541 ctgatgccag tggacgggtg gtccgcaaac tcacgggtaa aaatcagtct ggatttaacc
      601 gggtgacctg ggatcttcgt gaaccagaac cagagcttcc atcagaaacc caacccgatt
      661 tcgttgacga ggacggcggg acatttgggg cactggtcat gcctggacgc tataaggtaa
      721 gcattgccaa acgagttgac ggcgtactca cgcaactggc cggaccccag gaattttcag
      781 tcgtggcgga aggtactgac acaatgcccc aggctgatct ggcagcactc accgaattcc
      841 agcaaaaggc catgaagctg aaacgtgtgg tttcaggagc actcgaagtg gctggtgata
      901 caaaagatcg tctcaagaag atcaaacagg ccattcatga aacaccacag gctgacaaca
      961 agctgatgga cgatgcgctg agcattgaaa aacggctcaa cgccatcctg atagctctca
     1021 gaggcgacgt ggtgctccgt gcccgcaacg aaaacaccct gacttccatc tcggaacgga
     1081 ttgacaacat cctgagcgaa caacggatgt ccacctcacg tccgacccaa agccacattg
     1141 attcactcaa gatagctgat gaagaattca gggttgaact cgaaaagttg cgcacgctgc
     1201 tcgaagttga cctcaaaaac ctcgataaag cccttgaaac cgctggtgct ccctggactc
     1261 caggccgctt gccggaatgg aggtaggggc gttgtggctt tgcaccgcgc ctgacatctg
     1321 agctttgtgg gcgcagtaca cttttattct caaggccgga taacaggtgt gcagcgccct
     1381 tacatctatg tctttctttc acccgttgtt tctgtccttc gaagtcgccc tggccgccag
     1441 cacactggca ctggctgttg gggcgacttt tgcctggata ctggccaaac ttcggtttcc
     1501 tgggaaggag tggtgtgatg tgctcctgac cttgccgctg gtgctgccgc caacggtact
     1561 gggatattat ctgctggttg tgattggaaa tcggtcggtg attggtcgag cctgggaaag
     1621 ttgttttggc aatccactgg tgtttacccc gacagcagcc gtactggcgg catttatgtc
     1681 ggcgcttcca ctggtggtaa agtcagctcg ttctgccctg gaagcggttg agactgaagc
     1741 cgaaaacgcc gcccggttgc tgggtgcttc ggaatggcgc gtagcacgca ctatcacact
     1801 cccgctggcc tggaggggat tggctgccgc cggaaccctg gcctttgccc gagccctcgg
     1861 agatttcggc gccaccctga tggtcgccgg taaccttccc ggaaaaacac aaacggcagc
     1921 gctcgcgatt tatgatgcgg tcgctgccgg tcgtgattca gaagcgttga tgctctcagg
     1981 agtgatgtca gttgtggcgg cggtgctttt gtatggggtc aaccgcctga cggctgctca
     2041 tcataaatgg taagggaacc agggtgagag ttgaaaaaac gacaaaaaag acgagaagga
     2101 caacaaggac ctcaattcga aaccccggaa cccggaactc tgacaagatg accgactgac
     2161 aaggtaacaa ggtgatttct ttcatccctc atccctcatc cctcatccct tcaaaaaccc
     2221 cggaacccgg aactctgaca agatgaccga ctgacaaggt aacaaggtgn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tttctttcat ccctcatccc tcatccctca
     2401 ccccttcaaa acccccggaa cccgcttatt tcccttcttc cccaaccaca tcccgccgat
     2461 ccagaatggt atcaatcaag ccatattcca gagcctgggg tgcggttaaa taccggtcac
     2521 gttcaacatc ctgttcaacc agatcaaacg gtttcccact atggcttgag agcaagccgc
     2581 tgataatttc acgtaaccgg atgatttcat tggcataaat cttaatatcg gttgcctggc
     2641 cacgcatccc gcgcccgccc atatgcggtt ggtggatcat cacacaggtg ttcggaagtg
     2701 cacagcgttt gcccttggtt ccagcagcca gcaacaccga agccatactg gcacacatcc
     2761 caatgccaag tgtcacgatg tctggtttga caaactgcat ggtgtcgtaa atcgccagac
     2821 ctgacgtaat gctccctccc ggtgagttga tatagatatt gatatctttc tccgggtctt
     2881 ccgcctccaa aaacaaaatc tgagccacga cgaggttagc catatcatcg tcaatttcac
     2941 cattgatgaa gatgatattt tctttcagga gccgtgaata aatatcgaaa gcacgctcgc
     3001 cgcgcgctgt ttgctcaaca accattggga ccaacatggg gacctgtcct tttgtgcaat
     3061 gaaggtttgt gggtggattc cgaaaatcaa actcgatgtg taaggatgtt ttagtctact
     3121 ctgaggcatt gagacaaggc aggcatccgc tttatgcact tttcagtggc gatcaaaaaa
     3181 caatttacct caggacaaac tccatttgca ctcgatgtgg cctttactgt tcaggctggg
     3241 attatggtgc tctttgggcc gtcaggatct gggaaaagta caaccctcaa ctgcatt
//
LOCUS       DAOQDO010000454         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22245_length_3296_cov_3.623573, whole genome shotgun sequence.
ACCESSION   DAOQDO010000454 DAOQDO010000000
VERSION     DAOQDO010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22245_length_3296_cov_3.623573"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..2405
                     /locus_tag="PKZ53_08525"
     CDS             105..2405
                     /locus_tag="PKZ53_08525"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNJ40520.1"
                     /translation="MDLRSVAKQAVLVSLAYFLGARLGFALTFQPTPIAVLWPPNAIL
                     VACLLLIPPRHWGFMLGALLPVHLMVELGNHVPLLTVLGWYLTNCTEALLSASLLVWL
                     IPTRQLFDTFRGMVIFFLVAVVGSPLVTSFLDVGVVILTGFGKNYWQLLQARFLSNAL
                     AALMFVPVITLVVQQGKDWFTAITWGKMAEAGLLVIALSGYSFFTFGAEFRWNPSAQL
                     LSCTPLPLLLWAAVRFGTGGLSLSLFGTGVVAIWCAAHGLGPFTHQNPSANALSLQMF
                     ILFYAAPLLFLAALIKERQTNLEALHSSQTQAQRIFESSLIGIVFLDSEGNIIEANDT
                     FLQTVGYLRKDLFARTLQWTTLTPEPYRQLDQLALEEARHHGISRTYEKKLITCDRRQ
                     VSVLVRLAFLEKQATVSVGFVLDITEQTQVSARLEQLEDQFVKAFRSSPVVLVISRVS
                     DGNILEVNDRFEALLGYSRPEVIGKTSTELGLYDPGLRPVLLKQLDEQGYLRDYELDL
                     CDRWGNMLHTSVSFEQISLNSEACLLICVQDQTELKKADLALQELTARLLKLQDEERR
                     RIARELHDVTAQNISAISLNLAYLQKCLNLDDERPARAISESIELAEQVLKEIRTLSY
                     VLHPPLLDQSGLVPALQWYIDGFSRRSGVAVDFKVMTEIERLDPEIETTLFRVVQEGL
                     TNVYRHSGSSSARIRLLRDGDFIKLQIKDQGKGISFDEASAEPQLPGVGIPGMRQRIR
                     QIGGQLELISSPKGTVVTAIVPIKRN"
     gene            2408..3142
                     /locus_tag="PKZ53_08530"
     CDS             2408..3142
                     /locus_tag="PKZ53_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ40521.1"
                     /translation="MVRILIADDHDVMRRGIRNLLEMNVGWEICAEAATGREALDLAQ
                     QTKPDIAVLDLTLPDIYGLDVARDLLHIHAQTQILIFTMHESEQLVREVLSSGARGYV
                     LKSDAARHLILAIEALIDHKPYFSSTISETLMRAFLQTPLPGSASMKASSSVVTAEQL
                     NAEADDYFPPLGTDEPLIQAELTAREQQVVILLGKGKSNKEVAAELFISVKTVETHRA
                     TIMRKLGINSVVELVHYAIRTKLIDP"
BASE COUNT          764 a          805 c          852 g          875 t
ORIGIN      
        1 gttaacgcga agaattatag ctaatgcact atttggttat ggttttagca actataagcc
       61 ccaagccctc agccccacgt gatgcttgat ggatgaggtg tgacatggat cttcgtagtg
      121 tggcaaaaca agcggtgctg gttagtttgg cctatttttt gggcgccagg ctggggtttg
      181 ccctgacgtt tcagcccacc cccattgcgg ttttatggcc gcccaatgcc attctggtgg
      241 cgtgtttgct cttgattccg ccccgccact ggggatttat gttgggggct ttgctgccag
      301 tccatttgat ggttgaactc ggaaaccacg tcccgttgct gacggtcctg ggctggtatc
      361 tgaccaactg taccgaagct ctcttgagtg ccagtttgct cgtctggttg atcccaacca
      421 gacaattgtt tgataccttt cggggcatgg tcattttttt cctggtggca gtggtgggtt
      481 ctccgctggt gacctctttt ttggacgtcg gagtggtcat tctcaccggg tttgggaaaa
      541 attactggca gctcctccag gcccggttcc tttccaatgc cctggcggcg ttgatgtttg
      601 ttccagtcat tacattagtg gttcaacagg gaaaagactg gttcacggcg attacctggg
      661 gaaaaatggc tgaagccggc ctgctcgtca ttgcgttgag tggatactct ttttttacct
      721 ttggcgccga atttcgctgg aatccttcgg cgcagcttct gagttgtacc ccgttgccat
      781 tgttgttatg ggcggcagtt cgatttggta ctggcggact cagcctgtcg ttgtttggaa
      841 ccggagtggt ggccatctgg tgtgctgccc acgggttagg cccgtttaca catcaaaatc
      901 catctgccaa tgcactctca ctccaaatgt tcatcttgtt ttatgcggcc ccacttttgt
      961 ttctggcagc attgatcaaa gaacggcaaa caaatctgga agcgttacac tctagccaga
     1021 cccaggccca gcgaattttt gagtcaagtt taatcggaat tgtctttctg gatagtgagg
     1081 ggaatatcat tgaggccaac gatacgtttc tccaaaccgt gggctatctg cgaaaagatt
     1141 tatttgcccg gacgttacag tggaccacac tcaccccaga gccctatcgc cagcttgatc
     1201 aactggcgct ggaggaagcc aggcaccacg gtatttcccg aacctatgaa aagaaactca
     1261 tcacctgtga tcgccgtcag gtgtcagttc tggtgaggct ggcttttctt gagaaacagg
     1321 ccacggtttc agttgggttt gtgctcgaca tcaccgaaca aacccaggtc agtgcccggc
     1381 tggaacaact cgaagaccag tttgtcaaag cgtttcgttc cagcccggtg gttctggtga
     1441 tcagccgggt gtcagatggc aacatcctgg aggtcaacga ccggtttgaa gcgttgcttg
     1501 ggtattcgcg acccgaggtg attgggaaaa cgtcaactga gttgggttta tatgacccag
     1561 ggctccgtcc ggtgttgtta aagcagcttg atgagcaagg gtatttacgt gactatgaac
     1621 tcgatttgtg cgaccggtgg ggaaacatgc tccataccag cgtgtcattt gaacaaattt
     1681 cactcaattc cgaggcctgt ctgttgattt gcgttcagga tcaaaccgaa ttaaaaaaag
     1741 ccgatcttgc gttgcaggag ttaaccgccc ggttgttaaa actccaggat gaagagcgcc
     1801 gtcggattgc ccgcgagctc cacgacgtga cagctcaaaa tatcagtgcc attagcttaa
     1861 acctggccta cctgcaaaaa tgcctcaact tggacgatga acgcccggct cgcgccatct
     1921 cagagagtat tgagctggcc gagcaggtac tcaaagaaat tcgaaccctt tcgtatgtgc
     1981 tgcatccgcc actgcttgat cagtccggtt tggtcccggc actgcagtgg tacattgacg
     2041 ggttttcacg gcggagcggt gtggccgttg actttaaagt gatgaccgaa atcgagcgat
     2101 tagatcctga aattgagacg acgctgtttc gggtcgtcca ggaaggactc accaatgtct
     2161 atcgacattc tggaagttct tctgccagaa tcagactgct gcgtgacggg gatttcatca
     2221 agctccaaat caaggatcaa gggaaaggca taagctttga cgaagcttcg gctgaacctc
     2281 aattgcctgg agttggaatt cctggtatgc gccagcggat tcgccaaatc ggcggccagc
     2341 tagagcttat ttcaagtccg aaaggcacgg tcgtgaccgc gattgtgccg atcaaaagga
     2401 actaggtatg gttcggattc tcattgcaga tgatcacgat gtcatgcgtc gcggaattag
     2461 aaacctgctt gaaatgaatg ttggttggga aatctgcgcc gaggccgcaa ctggtcggga
     2521 agcactcgac ctggcacagc agaccaaacc ggatattgcg gttttggacc tgaccctgcc
     2581 agatatttat ggtctggacg tggcacgcga cctcttacac attcatgccc agacccaaat
     2641 cctgattttt accatgcacg aatcagaaca acttgttcgg gaggttttat cgagtggtgc
     2701 ccgtgggtac gttttaaaat cagatgccgc ccggcatttg atactggcca ttgaagccct
     2761 gattgaccac aaaccctatt tctcttcaac gatctctgaa acactgatgc gtgcgttctt
     2821 acaaacaccg ctgcctggtt cggcctcaat gaaagcctcc tcctcagtcg ttacggctga
     2881 gcaactgaat gccgaagccg atgactattt ccctccgctc ggcaccgatg aaccactgat
     2941 tcaggccgag ttaaccgcca gagaacaaca ggttgtcatt ttgcttggaa aaggaaaaag
     3001 taataaagaa gtggcggctg agttgtttat cagtgtcaaa acagtagaaa cccaccgggc
     3061 gaccatcatg cgaaaactgg gaatcaactc ggtggttgaa ctggtgcatt atgccatccg
     3121 caccaaattg attgatccat aaaactccat ccccgaaact ccaaataaca ctctagtctt
     3181 tcagaaaaag atttcctgaa ccattggtgg cgagagtttt agaaactatt gaaaaatatg
     3241 gacttatttt tcgtgtattc tgtggattcc gtggattctt tatctgaaat tctata
//
LOCUS       DAOQDO010000455         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22289_length_3293_cov_2.967881, whole genome shotgun sequence.
ACCESSION   DAOQDO010000455 DAOQDO010000000
VERSION     DAOQDO010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22289_length_3293_cov_2.967881"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKZ53_08535"
     CDS             complement(<1..987)
                     /locus_tag="PKZ53_08535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018398517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ40522.1"
                     /translation="MTDNTNKSNGEILIVDDNPINLDLLSGLLREHNYRVRAAINGRR
                     ALTAIRSCLPDLVMLDITMPEMDGYEVCRQIKADELTRDIPVIFISALDEVIDKVRAF
                     EIGGVDYVTKPFQFGEVLVRIENQLKISRLQREMERKNLELQQSYMQLERANRMLKAL
                     SYLDALTGIANRRHFDEALDQEWRRATRTAAPLSLVMIDIDFFKAFNDFYGHQGGDEC
                     LKQVAQVLSNTLKRAGDLAARYGGEEFAVILPGTEAEGAAILAEELRAKVEALRLEHQ
                     RAASQIVTISLGIATVLPKDGTSSESLIAIADRALYRSKKDGRNRVSVFDELL"
     gene            complement(1117..2553)
                     /locus_tag="PKZ53_08540"
     CDS             complement(1117..2553)
                     /locus_tag="PKZ53_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNJ40523.1"
                     /translation="MSTPSPSSAVPYALQYACICDRGLRRSANQDRAWADLDHRVFAV
                     CDGVGGSQGGEIASQTAIETLQDAFYTPDNNPPLIRLERAVYYANRDIFEMAARDVHL
                     AGMATTIVVLYLEGKTAYIGHAGDSRIYRFFQGKLTQETEDHTEVQEAVRRGSLTPEE
                     AVHARGKNIIMRALGIQPEIELETRSLTVEVGTRFLLCSDGITRHISNAEIEELMGRI
                     TDPQVLCDEFHRLCYSRGADDNLTALVVALNPHGDSITAPSPTPVTVDSIPSLDPVLV
                     HSGEHSFSAELRRSREHSIPPRSFPPERGGGKSHPFEEHLAAQKAALKRRLIGLAIGV
                     IFLVGLAFWAGQQSQTMRSPQQPDTPANLPGKSPTSPPSNQALFEAGQKAMTESNPKA
                     AQDIFSKLVNREPTRAQYHYWLGRAALANQQPQEAGKSLEKAIELDSKLADAYLYLAA
                     ARRMTGNLTGSEAALKEFEKRHQPAPSH"
     gene            complement(2703..>3293)
                     /gene="lpxI"
                     /locus_tag="PKZ53_08545"
     CDS             complement(2703..>3293)
                     /gene="lpxI"
                     /locus_tag="PKZ53_08545"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098629.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNJ40524.1"
                     /translation="AGQVKHKQIFSSTLPDLRMLMMLARLTMKNTDSLLGAVVGELEA
                     EGIRLIDSTYFVKHLLPVPGTLTRRSPNPKELADFTYGLHVAREIARLDLGQTIVVSN
                     CAVVAVEAMEGTDATILRAGELANGRPLTVIKVAKPNQDMRFDVPVIGLPTMNTLKRA
                     GATALTVTAGKTLLFDKEEMLNLANRYKITITAHEQ"
BASE COUNT          753 a          835 c          882 g          823 t
ORIGIN      
        1 gagcagttcg tcaaagacac tcactcgatt ccgaccatct tttttcgaac gatagagggc
       61 gcgatccgca atggcaatta aactttcaga tgacgttcca tctttgggta acacagtggc
      121 gattccaaga ctaatagtca caatttgaga cgctgcacgc tgatgttcca ggcggagcgc
      181 ttcgactttg gctctgagtt cctcggcaag gatggccgcc ccttcggctt cagtccctgg
      241 caaaataacg gcaaattctt cgccaccata gcgggcagcc aggtcgccag cccgttttaa
      301 ggtattgctg agcacctgag cgacctgctt gaggcattca tcaccgccct ggtggccata
      361 aaaatcattg aaggctttga agaaatcaat atcaatcatt accagtgaca gcggggcggc
      421 ggttcgggtg gctcgtcgcc attcctggtc aagggcttcg tcaaaatgac gtcggttggc
      481 aattccggtg agcgcatcca ggtatgacag agctttcaac atgcggtttg cccgctcaag
      541 ctgcatatag gattgctgaa gctccaggtt tttgcgctcc atctctcgtt gcagtcgcga
      601 gattttcagt tgattttcaa ttcgaaccag gacttcgcca aactgaaacg gtttggtgac
      661 atagtcaaca ccaccaattt caaatgcccg tactttatca atcacttcat cgagtgcgct
      721 gataaaaatc accggaatat cgcgggttaa ctcatctgct ttaatttgac ggcacacttc
      781 atagccgtcc atttccggca tagtgatatc cagcatcacc agatctggca gacaggaccg
      841 aattgctgtc agggcccggc gaccattgat ggctgcccgg actcgatagt tgtgctcgcg
      901 cagcaacccg gataacaaat cgaggttaat cgggttatcg tcaacgatca aaatttcacc
      961 attgctctta ttggtgttgt cagtcatgag ttcagcgttt caggggttca gggttttcgg
     1021 aagggatgag ggatgaaggg tgagggacga aaagatcacc ttgtcaccgt gtcattctgt
     1081 cagcctgtcg tccgagaaga gtattccaaa ctggtcttag tgggaaggtg ccggctggtg
     1141 tcgtttttca aattccttga gtgcggcttc gctcccagtc agattcccag tcatccggcg
     1201 ggctgcggca agataaagat aggcatccgc caattttgaa tccaattcga tggccttttc
     1261 aagggatttc ccggcttctt gtggttgttg attggctaaa gccgcacgtc caagccagta
     1321 gtgatattga gcgcgggttg gttcgcggtt gaccagcttg ctaaagatgt cctgcgctgc
     1381 tttcggatta gattcggtca ttgctttttg gccggcttca aacaatgcct gattagaagg
     1441 cggtgaggtt ggggattttc caggcagatt ggctggggtg tcaggctgct ggggtgaacg
     1501 cattgtctga gattgctggc cagcccagaa ggccagtcct accagaaaga ttaccccgat
     1561 agccaaccca atcaatcgtc gtttgagagc tgctttttga gcggccagat gttcttcgaa
     1621 tgggtgtgat ttgccgccgc cccgttctgg aggaaaactc cgaggtggaa tgctgtgttc
     1681 ccggctgcgt cgaagttcgg cggaaaaact atgctctccg gagtgaacca gcactggatc
     1741 cagagagggg attgaatcta ccgtgaccgg cgtcggggac ggcgccgtga tcgaatctcc
     1801 atgtggattg agcgcaacca ccagggctgt caggttatca tcggcgccgc gtgagtaaca
     1861 cagccggtga aattcgtcgc acaaaacctg tgggtccgta attcgtccca tcagctcttc
     1921 aatttcagca ttggagatat ggcgtgtaat cccatcacta cacaacaaaa atcgagttcc
     1981 tacttcaacc gtgaggcttc gggtttcaag ctcaatttca ggctgaatgc ccagtgcccg
     2041 cataatgatg tttttgccac gggcgtggac tgcttcctcc ggagtcaacg aaccacgccg
     2101 gacggcttcc tggacttcgg tatggtcttc agtttcctgg gtgagtttcc cttgaaaaaa
     2161 gcgatagatt cggctgtcgc ccgcgtgacc gatatatgct gtttttcctt ctaaatagag
     2221 caccacaatc gtggtagcca ttcctgccag atggacatca cgggctgcca tttcaaaaat
     2281 atcgcgattg gcataataca ccgcccgttc cagccggatt aaaggcgggt tgttgtccgg
     2341 tgtgtaaaac gcatcctgca gggtctcaat cgcggtctga ctggcaattt cacccccctg
     2401 gctcccgcca accccatcac aaaccgcaaa cacccggtgg tcaaggtcgg cccaggcacg
     2461 atcttgattg gcactgcgtc gtaacccacg gtcacaaatg caggcatatt gaagtgcata
     2521 ggggacggct gatgatggtg acggtgtgga cataggcagt cgtgaatagg agcgaaatcg
     2581 tgaagtcgtg aaggagcgaa gtcgtgaagg agcggaagag cgaaatctcg gcggaccaaa
     2641 caagtctgtc aagacctgaa tgctgtgagg ccactcgttc gctcgttcgc tattttactc
     2701 ttttactgtt cgtgggcagt gatcgtgatc ttataccgat ttgccagatt gagcatttcc
     2761 tctttatcaa aaagaagcgt ttttccggcg gtaaccgtta gtgccgtggc cccggcgcgt
     2821 ttgagcgtgt tcattgtggg cagtccaatc actggaacat caaagcgcat atcctgattg
     2881 ggtttggcca ccttgatgac ggtcaatggg cgaccgttgg caagctcacc tgcccgtaag
     2941 atggtggcat cggtaccttc catcgcttca actgccacca cggcacaatt gctcaccaca
     3001 atggtttgtc caagatcaag tcgcgcaatc tcacgggcca cgtgcaatcc ataagtgaaa
     3061 tcggccagtt cttttgggtt tggcgagcgg cgggtcaagg ttccaggaac tggcaagagg
     3121 tgtttcacaa aataggtgga atcaatcagg cggatacctt cagcttccag ctcgccaacc
     3181 acagcaccca gaagcgagtc ggtatttttc attgtgagtc gggccagcat catcagcatc
     3241 cgcaaatccg gcaaggtcga gctgaaaatt tgtttatgtt tgacctgacc agc
//
LOCUS       DAOQDO010000456         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22302_length_3292_cov_3.277417, whole genome shotgun sequence.
ACCESSION   DAOQDO010000456 DAOQDO010000000
VERSION     DAOQDO010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22302_length_3292_cov_3.277417"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKZ53_08550"
     CDS             complement(<1..564)
                     /locus_tag="PKZ53_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNJ40525.1"
                     /translation="MKNLTRLPQVSSQTFLKFLPYGLAVMGLVWVLHDVNPGQVFHQA
                     AYVNWWWVGLAVGCDTISYVSQGWRWHSLLPRPTRVTVGQTTQAVYAGLFASEILPLR
                     AGEIVRAMVVGKWANLPVTTIVPSIIVERLLDGFWLVVALSMAATWLPVPKQVVEATW
                     ILAGAMIVASGLGCLVVWQAGGISKTRL"
     gene            complement(584..1390)
                     /locus_tag="PKZ53_08555"
     CDS             complement(584..1390)
                     /locus_tag="PKZ53_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HNJ40526.1"
                     /translation="MGQKCFLAALCLVFSLVSPGAAQTFVTGKNTVLIRNKAQSVYYL
                     PAQGVSKHQKVLYFPGDGGWFGTGVTWAQMMASWGYDVYGVDTKQYLESFTSGSRTLS
                     EADICGDYRLLAQAITSDSGERLTIVGWSQGAGMGLLGGAPAANKPYFNGVIAIGLPE
                     TSVLGWRWKDNLTYVTGKDPDEPTFSSLEYLPQLAPLPFLMVNSTHDEYITPAQTKTL
                     FHSAAEPKMLRTIQANNHHFDGNTDGFYTALKDGLAWIAHLSTSTTGVQR"
     gene            complement(1312..3144)
                     /locus_tag="PKZ53_08560"
     CDS             complement(1312..3144)
                     /locus_tag="PKZ53_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNJ40527.1"
                     /translation="MDPMYQWRRQASHSLPPEHSSPRQYLAHRFQIRPWIRRIWESHA
                     IIWIVTLATLGSGILNIYSVIGPGLPYRVAILKEIFPLQFLPLSRLATLLIGMALVVV
                     SLNIYKRKTRAWYSVLLLASLSVMFHLTKGIDYEEALCSACLIGLLALVKARFQVKSS
                     IPTFPSALLRLGLVCSVVLLYGVAGFWLLEPREFGVNFTLAHSIHHTLNLLMLANEGL
                     SPHTRYARWFLESFDWLTISILFFGFWELYKPVIYRFRTHPQECEQARGIVEAHGRSA
                     LDYFKTWADKTFYFSETGHSFLAYRVSGRFALVLADPVGPEAEIEPLLRGFLKFCEEN
                     DWNVGFYQTLPDFLPIYQRLKFQHLKLGVDAVVDLATFSLSGSRMKKLRHLINHLEKE
                     HITFSIVTPPVAPEVMAQLSQVSNAWLGIPGRRERQFALGQFDVAYLQTTPIALAVDA
                     TGSVLAFANLIPSFSPGEATIDLMRHRPDAPNGIMDFLLINLFWFCRDQGYQRFNLGL
                     IPLLGQPNEVPTIGEKTLHRVSQPLQFFFSFEGLKRFKGKYATSWESRYAVFRTPFDF
                     PLEMLAFSQLAEFHQRRSSWVKSVFWQPCAWCFHWSRRVLLKHS"
BASE COUNT          867 a          865 c          849 g          711 t
ORIGIN      
        1 cagcctggtt tttgagattc cacctgcctg ccagaccacc aggcacccca gacccgaggc
       61 cacgatcatg gcgccagcca ggatccaggt tgcttcaacc acctgtttgg gaacgggcaa
      121 ccaggtggct gccatactca acgccacaac cagccagaac ccatccagca ggcgctcaac
      181 aatgattgag ggcacaatgg ttgtgaccgg cagattggcc cattttccaa ccaccatcgc
      241 ccgaacgatt tcgcccgccc ggagcggaag gatttcactg gcaaaaagcc cggcatatac
      301 cgcctgggtt gtctgaccaa cggtgaccct ggttggcctg ggcagcagcg agtgccaccg
      361 ccacccctgg ctgacatagc tgattgtgtc gcacccgacc gccagaccga cccaccacca
      421 gttcacatat gccgcctggt gaaaaacctg gcctgggttg acatcgtgga gtacccacac
      481 caatcccatc accgccagtc cgtaaggaag gaatttcaaa aatgtctggg aactcacctg
      541 tggcaatcga gtcaggtttt tcataaagtc acgggtcatc ttttcagcgc tggacgcctg
      601 tggttgaggt ggacagatgt gcaatccagg ccaggccatc ttttaaagcg gtataaaaac
      661 catctgtatt tccatcaaag tgatgattgt tggcctgaat tgtccggagc attttgggtt
      721 cggcggctga atgaaataaa gttttggttt gggctggggt gatatattcg tcgtgggtgg
      781 aattgaccat caaaaagggc aacggtgcga gttgggggag atactctaaa ctgctgaaag
      841 ttggttcatc cgggtccttg cccgtgacat aggtcagatt atctttccag cgccatccca
      901 acacgctggt ttcagggagt ccaatggcaa tcacgccgtt aaaatagggt ttattggctg
      961 ctggcgctcc acccagcaaa cccattccgg cgccctgtga ccagccgaca atcgtcaggc
     1021 gttcgccaga atcactggta atggcctggg ccaggagccg gtaatcgccg caaatatcag
     1081 cttcagaaag ggttcgactg ccggaagtaa agctctccag gtattgtttg gtatcaaccc
     1141 cgtacacatc atagccccag gatgccatca tttgggccca ggtgacaccc gtgccaaacc
     1201 agccgccatc tccaggaaaa taaagaactt tctggtgttt tgaaacccct tgggctggaa
     1261 ggtaatagac cgattgtgcc ttgtttcgaa tcagaaccgt atttttgccg gttacgaatg
     1321 tttgagcagc acccggcgag accagtgaaa acaccaggca cagggctgcc agaaaacact
     1381 tttgacccat gatgacctcc gttggtgaaa ttcagccagt tgactgaatg ccagcatttc
     1441 aagtggaaaa tcaaacggcg tgcgaaacac ggcataccgt gactcccaac tggtggcgta
     1501 tttgccctta aagcgtttga gaccttcaaa actgaagaaa aactgcagcg gttgtgaaac
     1561 ccggtgaagc gttttttcgc cgatagtggg gacttcattg ggttgtccca ggagcgggat
     1621 taacccgaga ttaaaccgct ggtatccctg gtcacggcaa aaccaaaaca ggttgatcaa
     1681 gagaaaatcc ataatgccgt tgggagcatc cgggcgatgc cgcatcaggt caatcgtggc
     1741 ttcacccggt gaaaatgacg gaatcaggtt ggcaaaagcc aggacggatc cagtggcgtc
     1801 aaccgcgagg gcaatcggcg tggtttgcaa ataggccaca tcaaactggc caagggcaaa
     1861 ctgccgttca cgacggcccg gaatcccaag ccaggcattt gaaacctggc tgagttgcgc
     1921 catcacttca ggggccacgg gtggggtcac aattgaaaag gtgatgtgtt ctttttccag
     1981 gtgattgatc aggtggcgaa gctttttcat ccggctgcca gaaagcgaaa acgtcgccag
     2041 atcaaccacc gcatccacac ccagcttgag gtgttgaaat ttgagccgct ggtaaattgg
     2101 aagaaagtca ggcagtgtct ggtaaaagcc aacgttccag tcattttcct cgcaaaattt
     2161 gagaaacccg cgcagcaacg gttcaatttc ggcttctggt ccaaccgggt cggccagtac
     2221 gagcgcaaac cgtccactga cacgataagc cagaaaactg tggccagttt ctgaaaaata
     2281 gaatgttttg tcagcccagg ttttaaagta atcgagcgct gagcgtccgt gggcttcaac
     2341 gatgcctctg gcctgctcac attcctgcgg gtgggttcga aaccgataaa tgacgggttt
     2401 gtacaattcc caaaagccaa aaaacaaaat gctgatggtg agccagtcaa atgattccaa
     2461 aaaccatcgc gcatatcgag tgtgtggtga cagtccttca ttggcgagca tcagcagatt
     2521 caaggtatgg tggatggaat gagccagcgt gaaattcacg ccaaattcac gtggctccag
     2581 caaccaaaac ccggcgactc catacagcag aaccacactg cacaccagcc ccagccggag
     2641 caacgccgaa ggaaacgtcg gaatactgct tttgacttga aaccgggctt tgaccagggc
     2701 taaaagtccg atcaggcagg ctgaacacag cgcttcttca taatcaatcc ctttggtcag
     2761 gtggaacata accgagagtg atgccagcaa caaaacactg taccaggcac gggttttgcg
     2821 cttatagata ttgagtgaca cgaccaccag cgccattcca atcaacaacg ttgccagacg
     2881 agacagcgga agaaactgaa gcgggaaaat ttctttcagg atggccactc gatacggaag
     2941 accaggacca atcaccgaat aaatgtttaa aatcccactg ccgagcgttg ccagcgttac
     3001 aatccagatt atggcgtgac tttcccaaat acgtcgaatc catggcctga tttgaaagcg
     3061 atgagccagg tattgacgag gggaggagtg ttctggcggt aacgaatgag atgcttgccg
     3121 tcgccactga tacatcggat ccataaaatt gcctcaccgg gttgaccatt cagtttcaat
     3181 gactgccact gtgcaagata tgttccacaa tcagtaggcg ccttccggtg ttgttttcct
     3241 cagcccaaaa gggtaagtgc cttaccgaaa atttccagac attttcacca cg
//
LOCUS       DAOQDO010000457         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22393_length_3283_cov_3.184944, whole genome shotgun sequence.
ACCESSION   DAOQDO010000457 DAOQDO010000000
VERSION     DAOQDO010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22393_length_3283_cov_3.184944"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKZ53_08565"
     CDS             complement(<1..546)
                     /locus_tag="PKZ53_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40528.1"
                     /translation="MQKCVAGNVCLHTRRKNSKNVTQGWFLPLLVLLFIGLSFVAGAF
                     VQAKEDSHLDLQLAARYFREAKELSDRDGGRTWGVPLYGPILLVDPETRQVVANQADT
                     AGNLKSNSGVFTGMLPADVTCANTATIWAGVEWTMMLWPLPSSQQERARLITHELFHR
                     IQEEIQFPASNPVNAHLESEAG"
     gene            complement(687..1607)
                     /locus_tag="PKZ53_08570"
     CDS             complement(687..1607)
                     /locus_tag="PKZ53_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40529.1"
                     /translation="MNRKQWIRVGCLIFCLMALMTAHPHVSEPTTATEDRLVRIQFQI
                     RAVEESARQRAILSESLIEGPPGTDFDITLQDDLFRLKAQFVTDVIAPGRLKIRGTLT
                     TKRLYGRSERQLPLYEEDSQVHSLQIGFDEAVVLFPFGRNGGDEVLKIEITPHVIEAS
                     GPSPNLVIKPIKTDLNGNIDIQARKVPHKFLTVAQLTKNGVVVAEGKSGCWLEEPQSI
                     QLVPLQIPNAPAVPGLVVDFTVNQVPYSQPNSTFGVNFDIRGTDQKVFARNWRGVGEF
                     GTPLQYTLPNEISVNDGEGWALKLIVQVEK"
     gene            complement(1604..2092)
                     /locus_tag="PKZ53_08575"
     CDS             complement(1604..2092)
                     /locus_tag="PKZ53_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ40530.1"
                     /translation="MNPHCELTEKVSQLIDGELAPEETSRVRAHLLVCPICRAAHSQF
                     LELRASLHTAETPTDFNRQVQALKTILATDKKLTWRHPLFIPWPATVGLVLISFLGGI
                     TFQSVINLKGSQSRVSEKRIQPVANPPAARGENRPFARFDRGERAELLVIRQPVKGEV
                     TQ"
     gene            complement(2089..2640)
                     /locus_tag="PKZ53_08580"
     CDS             complement(2089..2640)
                     /locus_tag="PKZ53_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ40531.1"
                     /translation="MPNDHDQFTHQFHELYPKLCRFLDGLVGKSACAHDLAQETMLRL
                     FQQSDVIPAEEIRFWVFRVARNLALNELERQRTRSKLSGWVASLFGSPRPTPETELVQ
                     KEQTNLVFSLIGRLPDHQRAVILLREQEDMSYSQIAEVLGVPESKVKIDLFRARMALR
                     EQFTLIRSQENRKNPPVRKGALP"
     gene            complement(2702..2998)
                     /locus_tag="PKZ53_08585"
     CDS             complement(2702..2998)
                     /locus_tag="PKZ53_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNJ40532.1"
                     /translation="MEIMTSWKEEGLQQGLQQGLQQGLQQGLQEGKKEGETTLLLLLL
                     QKRIGMVSEAITHQIKKLSLAQLESLAVALLDFHTEADLQAWFDANVTSIEGPN"
BASE COUNT          814 a          842 c          771 g          856 t
ORIGIN      
        1 cccggcttca ctctccagat gggcattgac tggatttgaa gctggaaact ggatttcttc
       61 ctggatgcga tgaaaaagtt catgggtgat caaccgggca cgctcctgct ggctcgaagg
      121 caatggccag agcatcatcg tccattcaac cccagcccat atcgtggcgg tattggcaca
      181 ggtcacatcg gcaggcagca tgccagtaaa caccccactg tttgatttca aattccctgc
      241 cgtatcagcc tgattggcca cgacctgtcg cgtctccggg tcaaccaaca atattgggcc
      301 gtacaatggc acgccccatg ttcgaccacc atcacgatca gaaagttctt tggcctcccg
      361 gaaataccga gccgcaagtt gcagatcaag gtgcgaatct tcctttgcct ggacaaaggc
      421 accagcaacg aagctgagcc caatgaaaag cagtaccagc aatgggagaa accagccctg
      481 agtcacattt tttgaatttt ttctccgtgt atgaagacac acgtttccgg ctacacattt
      541 ctgcatattc atacttctcc ttttttactt atggacggct cttataccat tttgcaataa
      601 agtcatcgta ttacgggaag gctaaagtat tgaaaaaatt gtctttccct gaaccctgaa
      661 cccgataccc tgaccccaaa atggtattat ttctcaacct gtactatcag ctttagtgcc
      721 cagccttccc catcattgac agaaatttca tttggcagtg tgtactgaag cggagttcca
      781 aattcaccaa ctcccctcca gtttcgggca aacacctttt gatccgtacc ccgaatgtca
      841 aaattcacac caaatgttga atttggctgg ctgtatggca cctggttgac agtgaaatca
      901 accaccagcc ccggaaccgc aggagcattt ggaatttgga gcggaaccag ttggatagat
      961 tggggttctt ccaaccagca gcctgatttc ccttcggcca caaccactcc atttttggtt
     1021 aactgggcaa ccgttaaaaa tttatggggt acttttcttg cttgaatatc aatattgcca
     1081 ttcaaatcag tttttatcgg tttgatcacg aggtttggtg acggtccaga ggcctcaatg
     1141 acatgcggtg taatttcaat ttttaagacc tcatccccac cattccgacc aaatggaaaa
     1201 agcaccacgg cttcgtcaaa cccgatttgg agcgaatgaa cctgggaatc ttcttcatag
     1261 agcggcaact ggcgttctga ccgaccatag agccgctttg tcgtgagcgt tccccgaatt
     1321 tttaaccgcc caggagcaat cacatcggtt acaaactggg ctttcagtcg aaataaatca
     1381 tcctggagcg tgatgtcaaa atcagtcccc ggcggcccct caatgagaga ttcgctcaaa
     1441 atggcccgtt ggcgcgcgct ttcttcaacc gccctgattt gaaactgaat ccgaaccagc
     1501 cgatcttcag tggccgtggt gggttcagat acgtgcgggt gcgccgtcat caacgccatc
     1561 aaacagaaaa tcaagcatcc aacccgaatc cattgtttcc ggttcattgg gtcacctccc
     1621 ctttgactgg ctgcctgatg accagcagtt cagcgcgttc ccctcgatca aatcgggcga
     1681 atggtcggtt ctcacctctt gcggcgggtg gattggcaac gggttgaatt cgtttttcag
     1741 atactctgga ctggcttccc ttcagattga tcactgattg aaaggtaatt cctccgagaa
     1801 acgaaatcag caccaaccca accgttgccg gccagggaat gaagagaggg tgtcgccagg
     1861 tgagcttttt atcagttgcc agaatagtct tgagtgcctg aacctggcgg ttaaaatccg
     1921 tcggtgtttc agcggtgtgc aggctggctc gaagttccaa aaattggctg tgggccgccc
     1981 ggcaaatcgg gcacaccaac aaatgcgccc gaacccgtga cgtttcttcc ggagccagtt
     2041 caccatcaat caattgggat actttttcgg ttaactcgca gtgcggattc atggtagggc
     2101 tccttttcga acaggtggat ttttccggtt ttcctgagaa cggatcagtg tgaactgttc
     2161 gcgaagtgcc atccgggcgc gaaacaggtc aattttgact ttactttcgg gtaccccaag
     2221 cacctctgcg atttgcgaat aggacatgtc ctcctgttcc cggagcaaaa tgactgcccg
     2281 ctggtggtct ggtaaccggc caatcagcga aaacaccaga ttagtctgtt ctttctgaac
     2341 cagttcggtc tctggcgttg gacggggtga gccaaataga gaagctaccc acccactgag
     2401 ctttgatcgg gttcgctggc gttccagttc attcaaggcc agatttcgag ccacccgaaa
     2461 aacccaaaac cggatctctt ccgctggaat gacgtctgat tgctggaaga gtcggagcat
     2521 tgtttcctga gccagatcgt gggcacaggc cgactttcca accaggccgt ccagaaagcg
     2581 gcacagtttg gggtacagtt cgtgaaactg gtgggtgaat tgatcgtgat catttggcat
     2641 agctgtttta gccagtaaaa cagctatgcc ggggaaaagt tacaggcggg agagaaaaaa
     2701 gttaatttgg gccttcaatc gaagtcacat tggcgtcaaa ccaggcttga agatcagctt
     2761 cagtgtgaaa atccaggagg gcaaccgcca gggattcaag ttgagcaagg gataactttt
     2821 tgatttgatg tgtgatagct tctgaaacca tcccaatgcg tttctggagc aataacaaca
     2881 aaagtgttgt ttcaccttct tttttccctt cttgcaagcc ctgctgtaag ccctgctgta
     2941 agccctgctg taagccctgc tgtaagccct cttctttcca actggtcata atttccataa
     3001 tttcctgcct ttcaccaggt tcaatacctt gcatctcaca gtgaaactca gtgagttcat
     3061 ttcctttcaa gttgagatat cgcctttcag aaaaagattc ccgttcagag taacgccttc
     3121 aggtgtgagg tttgctgagt ggaacgaatg atgaaaccct ctcatctaaa gacgatactc
     3181 tgaacctttt tctgaagagc tagagcattt ggaattagtt tggaagcatc agatttccaa
     3241 aggtttcgaa ataataggca aatttcacct gctgatgcgc cgg
//
LOCUS       DAOQDO010000458         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22490_length_3275_cov_2.168944, whole genome shotgun sequence.
ACCESSION   DAOQDO010000458 DAOQDO010000000
VERSION     DAOQDO010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22490_length_3275_cov_2.168944"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3245)
                     /locus_tag="PKZ53_08590"
     CDS             complement(<1..3245)
                     /locus_tag="PKZ53_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNJ40533.1"
                     /translation="MPQIEFYSAGPDSRFSTQDDIVARFPLGNPFRAVGVKINQAIQN
                     YDQREPGFLATFAALKAELKTLGVDFDALRDHWGHPYRLEWQILKEDYDDDSYFSVIV
                     KSAGPNGRFELPGDLTSDDFTVWAESVEQLDQLADPVDDIGWAYHRQYDHFPRNHTEW
                     NQALRLPRRFHQKLIDPWGRPFLLKFEVKTDRVFQIPQAQPGHSEYLASFHWPLIPTG
                     TMYLRKAVVSFSSSGPDRRPGTTDDFIALTYEEPILPIVKLHSTSNRRLSGHQPRKAG
                     LIAGILTDGTGVALAEESVEIRMDSWKEGIKARTDVEGKFQFVDLQPGLYSLNIQMAG
                     YRPVLISNISVIAGYLTPICQKIVLQPYDPSTASEVISVTAGKTGPDLPYQKGPARSD
                     TSQLLTTPRIRKDFPETLYWLPELVTDQQGRAQVQFPVADAMTTWKCAVIATTLDGRQ
                     AMTQTDVQVTQPFFVEADLPSHLTEGDIIHLPVVVRNYLPVPQTITVELKLPPEVRLV
                     GQPHQQLQLQANTSTRLFFQIRAEKPLTDGRVQIIARGSQFSDAVEQPFSVRPDGPEI
                     LQTVSRNFTGHTQVEVVIPASARKTQSRAELKLYPNAISHVLTCLTGMLQQPYGCAEQ
                     TISTTIPNLLLLHYLKLNHIEHHPQEGFALHNLQAGYQRLLQFQHNDGGFGYFPTSSR
                     DMALTAYVMHFLQDAQSFVVVDRNVLHTAGNWLLRQQKPDGSWAGSPQLTGYIARMLT
                     QLPDASDSVERALQYLSTQAETTQEAYPLAQIALAAQAASHQPLAQKVISRLKALAIP
                     AESAVFWNNPQETLFFGYGKTGRLETTALALEALGKTDASSGLIGKGWLYLLENKDFY
                     GGWYSTYATAQTLRALQTAFGSQQTTDTRIKVLVNGEQVTEFDLSARNDQPMAINLAE
                     KLTAEHNTIELQQTGGQLPLTMQVTAPYFLSWESAKSALTQHSATRGKLTLKVGFSQT
                     NAPTGTPITCAVDISRQFSPDAYHHGMYLAEIGLPPGAIVDRESLNRALQQSSGSIHQ
                     YELQPGRVIFYLNSWNHQAQFTFSFSLKFPLTAQSMPFMVYDYYNPEEQVTV"
BASE COUNT          781 a          771 c          902 g          821 t
ORIGIN      
        1 accgtgacct gttcttcagg attgtaatag tcatacacca taaacggcat tgactgggcc
       61 gtaagtggaa atttcaggga gaaagaaaag gtaaattgcg cctgatgatt ccaggaattg
      121 agatagaaaa tgacgcgacc aggctgaagt tcatactggt gtatgctgcc tgaagattgc
      181 tgcagtgcgc gattgagtga ctcccggtca acaatcgcac ctggtggtaa cccgatttct
      241 gccagataca tgccgtgatg ataggcatcg ggtgaaaatt ggcgggagat atccaccgca
      301 caggtaatcg gcgtgccagt tggggcattg gtctggctaa accccacttt caaggtcaac
      361 ttgccgcgag tggctgagtg ttgagtcagg gctgatttgg cagattccca cgagagaaaa
      421 tagggagctg tgacctgcat tgtgagtgga agctgaccgc cagtttgctg gagttcgatg
      481 gtgttgtgtt cagccgtcaa tttctcagcc agattgatgg ccattggctg gtcatttctg
      541 gctgacaggt caaactctgt cacctgttca ccattgacca ggactttgat tctggtgtca
      601 gtggtctgct gtgaaccaaa tgcggtttgc agggccctga gtgtttgggc cgtggcgtag
      661 gttgaatacc agcctccgta aaaatcttta ttctcaagga gatacaacca gccttttccg
      721 atcagtccag aactcgcatc agttttccca agtgcttcaa gcgccagagc tgtcgtttca
      781 agtcgtccag tttttccata cccaaaaaac aaggtttctt gtggattgtt ccagaagaca
      841 gcggattcgg caggaatcgc cagggctttg agtcgtgaaa ttactttttg agccagcggt
      901 tgatgagaag cagcctgggc cgcaagtgct atttgggcca ggggataggc ttcttgcgtg
      961 gtttcagcct gggttgaaag atactgcaat gcccgctcca cagaatcgga tgcgtctggg
     1021 agttgggtca acattcgagc gatatagccg gtgagttggg gagaacccgc ccagcttcca
     1081 tctggctttt gttgtctgag caaccagttt ccagcggtgt gaaggacatt tcgatccaca
     1141 accacaaatg actgggcgtc ctggagaaag tgcattacat aggctgtcaa tgccatatca
     1201 cgggaactgg taggaaaata tccaaaccca ccgtcattgt gctgaaactg gagcagccgt
     1261 tgataacccg cctgcagatt gtgcagggcg aacccttctt gcgggtgatg ctcgatatga
     1321 ttgagtttca ggtagtgcag caggagcaga ttgggaatcg tggttgaaat tgtctgctcg
     1381 gcacagccgt agggctgctg gagcatccca gtcaggcagg tcaggacgtg tgaaatggca
     1441 tttggataga gtttcagctc tgccctggat tgggtcttcc gtgctgaagc cggaatcaca
     1501 acctcaacct gggtgtgacc ggtaaaattt cgactgacgg tctgcaggat ttccggtcca
     1561 tcaggtcgaa ctgaaaaagg ctgttccacc gcatcactga actgacttcc acgggcgata
     1621 atctggactc gtccatccgt gagtggtttt tcagcccgaa tttgaaaaaa caggcgggtt
     1681 gatgtattgg cttgaagctg gagttgttga tgcggttgac ccactagcct gacttcgggt
     1741 gggagtttga gttcaacggt gatggtttgg ggcactggca aatagttgcg taccaccacg
     1801 ggcagatgga tgatatcccc ttcggtcaaa tgggacggca ggtcggcttc gacaaaaaac
     1861 ggctgggtga cctgcacgtc ggtttgggtc atcgcctgac ggccatcaag cgtggtggca
     1921 atcacggcac atttccaggt ggtcatggcg tctgccacgg gaaattgaac ctgagcccga
     1981 ccctgctggt cagttaccag ttctggtagc cagtagagtg tctctgggaa gtctttgcgg
     2041 attcgtggcg ttgtcagcaa ctggctggtg tctgaccgtg caggtccctt ttgatatgga
     2101 agatccggtc cagtctttcc agcggttact gaaatgactt ctgaagccgt gctcggatca
     2161 taaggctgga gtacaatttt ctggcagatg ggtgtcagat aacccgcaat gactgaaatg
     2221 tttgagatca acacaggtcg gtatccggcc atctggatat tgagtgaata caaccccggc
     2281 tgaagatcta caaactggaa tttaccttcc acatcagtcc tggctttgat cccttctttc
     2341 caggaatcca ttcgaatttc aactgattct tcagccagag ctactccagt tccatcggta
     2401 aggattccgg ctatcaggcc agctttccgt ggttgatggc cactgagccg acgatttgac
     2461 gttgagtgaa gtttgacaat tggaaggatc ggttcttcat atgtgagcgc tataaaatca
     2521 tcagtcgttc caggccgccg atctggtcca cttgacgaaa acgaaaccac ggctttccga
     2581 aggtacattg tcccggttgg aatcaacggc cagtgaaacg acgccagata ctcggaatgt
     2641 ccaggttgag cctggggaat ctggaacacg cgatctgttt tgacttcaaa ctttagtaaa
     2701 aagggacgtc cccagggatc aatcagcttt tgatgaaacc ggcgaggcag tcgaagcgcc
     2761 tgattccatt cagtgtgatt tcttggaaaa tggtcatact gacggtgata cgcccagcca
     2821 atatcatcca ctggatcggc cagttggtcg agttgctcaa ctgactcggc ccaaactgtg
     2881 aaatcatcgc tcgtaagatc gcctggcaat tcaaaccgac catttggccc ggcacttttg
     2941 acgatgaccg aaaaataaga atcatcatcg taatcttctt tcagaatctg ccactccagc
     3001 cgatacggat gcccccagtg gtcgcgcagg gcatcaaaat caaccccaag tgtttttaat
     3061 tcggccttga gtgcggcaaa ggtggcaaga aaaccaggtt cacgctggtc ataattttgg
     3121 attgcctggt tgattttgac cccgacagcc cggaagggat ttcctaacgg aaagcgggcc
     3181 acgatgtcat cctgggtcga aaatctcgaa tccggcccgg cggagtaaaa ttcaatttgt
     3241 ggcaacccga cagcgtttgg aaaaacacga atctg
//
LOCUS       DAOQDO010000459         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22509_length_3273_cov_3.065258, whole genome shotgun sequence.
ACCESSION   DAOQDO010000459 DAOQDO010000000
VERSION     DAOQDO010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22509_length_3273_cov_3.065258"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3273)
                     /locus_tag="PKZ53_08595"
     CDS             complement(<1..>3273)
                     /locus_tag="PKZ53_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40534.1"
                     /translation="LVQTLTSEKLNFGDEIWLAIRFLLHCESSQARNRDGLFLSESTD
                     IWKRLIEQILTADGKNESWRLLPIEWDSKLNSKNKRDLQISMVDAAGVLKELQSGRVN
                     IDHLKFEPDVWSPSDISKILGGLYHESDDSSSEKESRIKLLRKLRLHTIKGQINERVT
                     VAKSNGDLDESFVLEATDFEKSIPDDLKPQWQLFLNEIKVVERFSDDYPVAARAQKSI
                     FSRKVGSNEEPYLVELDWNYVVRRSLASLKPGDWVELILLALEQKGGQAAKGIGKELK
                     EKKWLPLSQGGFISPDSMIHIEGLEDNLHQLLDPEKDGLAGINHLPEWIRSHRGFASL
                     KTFFLLGEQAIESLGLWLEEKPDWILGLTHSFQMSELKPILEQLQHIETLPVVSFLLK
                     LQSTYLSGKDEKKTENLEKWIEEYILPKVLKPFDYQKDGVEKVKDILLVLQGHKDVKV
                     FEVYLAQAKRDSVLESFLPALSLVNKVEQWIPASKLVWPSENFPPEYQLCQRQADILA
                     VQLNQNESAAGSFDESGDLPIIQSKGELSQNPNFADQADKLAKYLQPFRNGNIGDNLP
                     CALVAVLAGHGKIKKLLKQLLNANLKQHPEDFIAWLLGDKHEDLKRSLEREKFLIDIV
                     RGTSAKAKTITGEEIPVSLTQEISTLIVGNPRDLWRSHTFQGRPETACHILRLRWVEN
                     PDTLQDPVGVFASTIETILLRVHCNGANHLVPQGIKNLLDRIADAGQADIRRSRSFLL
                     DMAEIRLNELGLKTDRIFEGILKNFNAARQARITAEDLYERNRSISDKKRQESRELLK
                     KANEELMSLMESEENSSRQRLIEALRRKMKDYQYGIDSVTLELFQNADDAVAEWKEMK
                     KTLDPQEQRFVLRFDGQQKVLEIVHWGRPINQHAFPGFEAGSRGYDQDLQKMLALNFS
                     DKGIELDDRPNIVTGRFGLGFKSVFFISDQPEIISARLAFEIRGGFLPMPLSSKVAEE
                     MRTRVPETGPQPTMIRLKVAESVDLGSVEDSVEKFTRFAPFLSIFSRQIKELTISKNG
                     HETHWRNVEVKLTENGGVTVSHLGSDKSYLCFRCPMRSDDRPATILFQLSPDGIKGVT
                     DE"
BASE COUNT          859 a          766 c          663 g          985 t
ORIGIN      
        1 tctcatctgt caccccttta atcccatccg gggataattg aaataaaatg gtggctggcc
       61 ggtcatctga acgcattgga catctgaaac agaggtatga tttatcgctt ccgaggtgag
      121 acactgtcac cccaccattt tcagtcaatt ttacttcgac attgcgccag tgcgtctcat
      181 gaccgttttt tgagatggtt aattctttga tttgtcgaga gaagatggat agaaatggcg
      241 caaaccgggt gaatttttca accgagtcct caactgaccc gagatctact gattcagcaa
      301 ctttaagccg aatcattgtg ggctgagggc cggtctcagg taccctggtt ctcatctcct
      361 cggccacttt acttgataat ggcatcggaa gaaaccctcc ccgaatttca aaggccaatc
      421 gagcactgat gatttcaggc tgatcagata tgaaaaatac acttttgaac ccaagcccaa
      481 atcgtcccgt tacaatattt ggtcggtcat ccagctcaat gcctttatct gagaaattaa
      541 gggctagcat tttttgaagg tcttgatcat acccccgtga cccagcttca aaaccaggga
      601 acgcatgctg attgattggt cttccccaat gaacaatttc aagaactttt tgttgaccat
      661 caaacctgag aacaaaccgc tgttcctgag ggtctaaagt ctttttcatt tctttccact
      721 cagccacggc atcatctgca ttttgaaaga gttccagggt aactgaatca attccgtatt
      781 ggtaatcttt cattttgcgg cgaagtgcct ctatgagtct ctgacgagac gaattttctt
      841 cagattccat caatgacatt aactcttcat tggctttctt taacaattcg cgagactctt
      901 gcctcttctt gtcagaaata gaccgattcc gttcatatag atcttccgca gtaatccgag
      961 cttgacgagc tgcattaaag tttttgagga ttccttcaaa tattcggtca gtcttcagtc
     1021 ctaattcatt caaccggatt tcagccatat ccagaagaaa actgcggctt cgtcgaatat
     1081 ccgcctgacc agcatcagca atacggtcga gcaggttttt gataccttgt ggaaccaggt
     1141 gatttgctcc attacagtgg actctcagca ggattgtctc aatggtggaa gcaaagaccc
     1201 caaccggatc ctgcaacgtg tctggatttt caacccaccg aagccgcaaa atgtgacagg
     1261 ctgtttcagg ccggccctgg aaggtatgtg accgccaaag atctcttgga ttaccgacga
     1321 taagcgtgga aatctcctgg gtaaggctta cgggtatctc ttcgcctgta attgttttgg
     1381 ccttcgcact tgtacctcga acaatgtcaa tcaaaaattt ttcccgctcc aaggatcgtt
     1441 ttagatcttc gtgtttatca cctaacaacc aggcgataaa atcttcagga tgctgcttga
     1501 gattggcgtt aagcaattgt ttcagtaact tttttatttt tccatggcca gccagaactg
     1561 ctaccagagc acagggaaga ttatcgccaa tatttccatt cctgaaaggc tgtagatact
     1621 tggccagttt gtcggcctgg tctgcaaaat tgggattttg acttaactca cctttagact
     1681 gaattatggg taagtcccca ctctcatcga aggagcctgc cgccgattca ttctgattta
     1741 attggacggc caaaatatct gcctgtcttt ggcagagctg atactccgga ggaaaatttt
     1801 ctgatggcca caccagtttg gaggctggaa tccactgctc taccttattc actaaggaaa
     1861 gggctggtaa gaatgactct aaaacactgt ctctttttgc ctgggccaga tatacctcaa
     1921 atactttgac gtctttgtgc ccctggagca ccaacagaat atcttttact ttttctacac
     1981 catctttctg ataatcaaag ggttttagaa cctttggcaa aatatattct tcaatccatt
     2041 tctcaaggtt ctctgttttc ttctcgtctt ttccagacaa gtaagtgctt tgtaatttca
     2101 ataagaatga gacgactgga agggtttcga tatgttggag ttgttcaagt attggtttta
     2161 actcagacat ttgaaaagaa tgagtcaggc caagaatcca gtctggtttt tcttcaagcc
     2221 atagccccaa agattcgatt gcttgttctc caagtaggaa aaatgtcttg agggaagcga
     2281 aaccacgatg tgacctgatc cattcaggca agtggttgat tcctgccaga ccatcttttt
     2341 caggatcgag gagttggtgg agattatctt ccagtccctc aatatgaatc atgctgtctg
     2401 gtgaaatgaa tcctccctga gacaacggaa gccatttctt ttcttttaac tcttttccaa
     2461 tccccttagc agcctgacca cctttttgtt ccagagctag cagaataagt tccacccagt
     2521 cacctggctt aagggaggct aaagaccgac gcaccacata attccaatcc aactcaacca
     2581 gatagggttc ttcatttgac ccaaccttcc tggaaaagat agatttttga gctcttgcag
     2641 ccaccggata atcatcagaa aatcgctcta cgacctttat ttcattcaga aacagttgcc
     2701 attgtggttt tagatcatcg ggaattgatt tttcaaaatc agttgcctcc aatacaaaag
     2761 attcgtctaa gtctccattg ctctttgcga cagtgactct ttcattgatt tgtcctttga
     2821 ttgtgtgtaa ccgcagtttt cgcagcaact tgattcggct ctctttttca gaggaactgt
     2881 catcagattc atggtataac cccccgagaa tcttagagat atccgaaggt gaccatacat
     2941 caggttcaaa tttcaagtgg tctatattca ctcgacctga ttgcaattct tttaaaactc
     3001 ctgcggcatc aaccatggaa atctgaaggt cacgtttatt cttagaattc aatttagaat
     3061 cccattcgat gggtaagagt cgccaggatt cgttctttcc atcagcagtg agaatttgct
     3121 caatcagtcg cttccatata tctgttgatt cagagagaaa tagcccgtcc ctgttgcggg
     3181 cctgagatga ttcacaatga agaagaaacc gaatggccag ccagatttca tccccaaaat
     3241 tcagtttttc tgaggtcaat gtctggacaa gag
//
LOCUS       DAOQDO010000460         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22576_length_3267_cov_3.518680, whole genome shotgun sequence.
ACCESSION   DAOQDO010000460 DAOQDO010000000
VERSION     DAOQDO010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22576_length_3267_cov_3.518680"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKZ53_08600"
     CDS             complement(<1..201)
                     /locus_tag="PKZ53_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ40535.1"
                     /translation="MSSPNAQTPCITLFTRDNQPLSREITGDVFSIGRGPDNDLQIKD
                     LLISRNHAEIIRSADGTFVFRDK"
     gene            583..3066
                     /locus_tag="PKZ53_08605"
     CDS             583..3066
                     /locus_tag="PKZ53_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40536.1"
                     /translation="MRRLWMDLQFAGRMFARYPGFYAIALITLAFGIGANTTIFSVIN
                     ALLIQPLPFSEPERLVQVSQSGPSPQQPDGMVYWSFPHFKALREANSCFENAAAFVEQ
                     PLNLTGTDGAERVRVEMVSQSYFPVLGVQAAVGRTFLPEEDQTPGSHPVALISHALWQ
                     RRFGSAPDTINKALELEQKVFTIVGILPAGFKGQHGSVDIWVPTQMATVLRYPKILTN
                     SNNSWLQVIARLKPGLSVSQAQSALASTKDHLDQLFPASKAGAAAPLGLIPLHQTKVD
                     PQLKLSLLILMAAVGCVLLIVCANTANLFLVRVVERQKEMALRLMLGASRSQLVQQFL
                     TESLVLSLIGGSLALVVATWGIDLLLAFKPTDEEQFWTVYARIFDLFSIRLEPTVLLF
                     HFAISAGTGLLFGLAAAFRVSLTNPLQSLKSNDHSFRFQLTRLASARRVNLRTLLVIG
                     EIAISMVLLVGTGLMIRSLLQLQAVHLGFSPQHVLTVQLPSRNAPLEFYEQAVSTVAA
                     LPGVEVVGLGSSAPLMGYSSLTKMTIEGQADANQAFTIGFHSVSPGYFQSLQIPIVSG
                     RTFTTQDRIGAPRVAVINRTAAQRYFAGTDPLHRRIRPMVGAEYPEAEELIEIVGVVE
                     DVRYGRLEEKIGPDVYLSAWQPTDPAQTLVIKASGDPQFLVAGITTEIKRLNPNVPVA
                     NIQTLQERTVEVTSRTRFIALMLGIFAAVAAFLAALGVYGVLTFSISARTREIGTYLA
                     LGAQPRDILHMVATEGAVLIGLGLVLGVCGALGSLHILQSQLFEISSTDPVTVLAVTS
                     FLAVTGFLACLIPAYRATRIDPMVTLRHE"
BASE COUNT          739 a          823 c          825 g          880 t
ORIGIN      
        1 tttgtcccga aaaacaaagg taccgtcagc agaccgtata atttccgcat ggttgcgcga
       61 aatcaataaa tctttaattt gcaaatcgtt atctggtcca cgtccaatcg aaaaaacatc
      121 gccagtaatt tctctggaga gcggctgatt atccctggta aacaaagtga tgcacggggt
      181 ctgagcattt ggcgaagaca tggcgaattt tgtggggatt ccttcagaaa tgagatgttg
      241 tcgtgagcgg ctattctaaa tcagacaggc atttaaaaga agcatttcgg ggaacccaat
      301 ttcacctcgc cagcagtttg gagaataacc ctgacctgaa aagcggctga ctggcttaga
      361 tgcttcctga tgttttgttt ttgagactca acattcccat taccgaacaa tctctgaggt
      421 gggcagtcac ggctgtgatt tgagattgtg cccacctgcc ttgtacaacc caggtcttga
      481 aagactggca cacgacttgt tctccctgca ttcagtcaac acctctttcc aaaattcctg
      541 ccgttgaact cactgttcca ggacgacctc aaggagaaca ctatgcgacg actgtggatg
      601 gatctgcaat ttgctggacg aatgtttgcc aggtaccctg gcttttacgc gattgcactg
      661 atcacactgg cgtttggtat tggtgcaaac acaaccatct ttagtgttat caatgccctt
      721 ttgattcaac cgttgccatt ctctgaaccc gaacgactgg ttcaggtgtc tcaatcaggc
      781 ccgtctccac aacaacctga cggcatggtg tactggtcct ttccgcactt caaagcgttg
      841 cgcgaggcaa actcgtgttt tgagaatgca gccgcctttg ttgaacaacc attgaatttg
      901 actggaaccg acggcgccga acgagtccgg gtagagatgg tttcccaaag ctattttcca
      961 gttcttggcg tccaggcggc tgtcggaaga acatttcttc ctgaagaaga ccagacaccg
     1021 ggctcacatc cggtggccct gatcagtcat gcattatggc aacggcgttt tggcagtgcc
     1081 cccgacacaa tcaacaaagc actcgaactc gaacaaaaag tgttcacaat tgtcgggatt
     1141 ttgccggctg ggtttaaagg acagcatggc agcgttgata tctgggtccc aacccagatg
     1201 gcgactgtcc tgcggtatcc aaaaatttta accaactcga ataattcctg gcttcaagtg
     1261 attgcccgtc tcaaacctgg actgagcgtt tcacaggcac agtcggcact ggcgtccacc
     1321 aaagaccatc tggatcaact ctttccagct tcgaaagctg gcgctgctgc accactggga
     1381 ctcatccccc tgcaccagac caaagtggac ccacaactca agctgtcatt actcatcctg
     1441 atggcagcag ttggatgtgt gctcctgatt gtgtgcgcca atacggccaa tctgtttctg
     1501 gtccgggtgg tcgaacgcca aaaagaaatg gccctgcggt tgatgcttgg ggcaagtcga
     1561 agccagcttg tccagcaatt tctgactgaa agcctggttc tgtccttgat cggtggtagc
     1621 ctggcactgg tggtggcaac ctgggggatt gacctcttgc tggcgtttaa acccactgat
     1681 gaagaacaat tctggacggt ttatgcccgt atttttgatc tgttttcgat tcgcctggaa
     1741 ccaacggtgc tgttgtttca ctttgccatc tcagcaggta caggtctgtt atttgggttg
     1801 gccgccgcat ttcgggtgtc cctgaccaat ccgcttcaat cactgaaatc caatgatcat
     1861 tcgtttcgct ttcaattgac tcgtttggca agtgcccgac gggtcaacct gcgaacgttg
     1921 ctggtgattg gtgaaattgc aatttctatg gtgttgctgg ttgggacggg attgatgatt
     1981 cggagtctgc tccaactgca ggcggtccac cttgggtttt cgccacagca tgtgctcacg
     2041 gttcaattgc cgtcgcgcaa cgctccactt gagttttatg agcaggctgt ctccacggtc
     2101 gcagccttgc ctggtgttga agtagtcggc ctgggaagct cagcaccgct gatgggctat
     2161 tcaagtttga ccaaaatgac gattgaaggg caagctgatg ccaatcaggc tttcactatc
     2221 gggtttcaca gtgtttcgcc tggttacttc caatcactgc agattccaat tgttagcggg
     2281 cgaactttta caacgcagga tcgaattggg gcgccacggg ttgcagtcat caaccggact
     2341 gccgcccaac gctattttgc tggcaccgat ccactgcacc gacgaatccg tccaatggtt
     2401 ggagctgaat accctgaagc cgaagaactg attgagattg tgggtgtggt cgaagatgtc
     2461 agatatgggc gacttgaaga aaaaattggc ccagatgtgt acctttcagc ctggcaaccc
     2521 actgatccag ctcaaacact ggtgatcaaa gcgtctggtg atcctcaatt cctggtggct
     2581 ggtatcacaa ctgagatcaa acggctcaac ccaaatgtcc cggtggcaaa tatccagacc
     2641 cttcaagaac gaacggttga ggtgacttcc cgaaccaggt tcattgcgct gatgcttggg
     2701 atttttgcag ccgtagcggc atttttggcg gcgcttggtg tctatggcgt attgactttt
     2761 tcgatctctg cccgaacccg cgaaattggc acctatctgg ctctgggcgc tcaacctcgg
     2821 gatattctgc acatggtggc cactgaaggt gccgttttga ttggcctggg gttggttttg
     2881 ggagtatgtg gtgcgttagg atcgcttcat attttgcaaa gccagctttt tgaaatttca
     2941 tcaaccgatc cggtaaccgt tttggcagtc accagtttcc tggccgtgac cggatttctg
     3001 gcctgtttga tcccagccta tcgggcaacc cgaattgatc cgatggtgac cttacggcat
     3061 gaatgatggc aggcagggtt ccgggttccg ggttccgggt tttcgattgt ttgcccgtgt
     3121 tgtccctttt aaacctcaaa ccctactgaa tatacccctg aattagacca ttttggaatg
     3181 gagtcctcgt attacgggaa gtctaaatta ttgaagaaat tgtatttccc tgactcttga
     3241 ccccgagcct ctgatcccaa agtggta
//
LOCUS       DAOQDO010000461         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22595_length_3266_cov_2.219558, whole genome shotgun sequence.
ACCESSION   DAOQDO010000461 DAOQDO010000000
VERSION     DAOQDO010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22595_length_3266_cov_2.219558"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKZ53_08610"
     CDS             <1..290
                     /locus_tag="PKZ53_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40537.1"
                     /translation="GSRSASPYGPQEIVSEGRVARKFTQHCEKFYSEVESLLPPACAI
                     YICGPGEAKLEFNKHLARNKNLAEHIRDIEPADELTETQIVACVKEYFQIA"
     gene            complement(479..814)
                     /locus_tag="PKZ53_08615"
     CDS             complement(479..814)
                     /locus_tag="PKZ53_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40538.1"
                     /translation="MVLFFTLAAIRDALHKRLPIVGQLQPNFLEYVGLSTEIGVIYSF
                     LSGLVSFTTYCLVRENTRFFKLRNHFDVNDLVLLGAVLIGSLVLFSLCGSLLLCSVLW
                     KRVHKTGIV"
     gene            complement(1078..1617)
                     /locus_tag="PKZ53_08620"
     CDS             complement(1078..1617)
                     /locus_tag="PKZ53_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40539.1"
                     /translation="MKWFNIDLTNPNLDQKDVHTVDFPSFWDYVTLYLLCFPWFSIGV
                     GTHEPAIGAWTVALLVTLIFLMHDICHLIHQFGQSPADLLKFELRCVAMGLTYGFCSG
                     LVSFVTFCIVRDIWSLFTLNAHAFRIEHLPIGAGLVIGMLTFASTLSGLLFSSLFWKR
                     RKRANRNQCKRSDFGRCSR"
     gene            complement(1614..2174)
                     /locus_tag="PKZ53_08625"
     CDS             complement(1614..2174)
                     /locus_tag="PKZ53_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40540.1"
                     /translation="MIELFLARRNELNSVIEGNARLCFLLPVFNTQKVMAAIEFCLEP
                     GDGTITFSDYYFFHFSGVTSITIGTLQSQYFPPELKNVSTPSIQLMELLLRQFRDQMI
                     FEWYCFYARDEVLVEKFTPRLHFQFDFASPDAGEMAGFLTDVYLTGWQVWINYSELTI
                     ENHLRQSLTVEQFTESARRFLESTER"
     gene            complement(2193..2366)
                     /locus_tag="PKZ53_08630"
     CDS             complement(2193..2366)
                     /locus_tag="PKZ53_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40541.1"
                     /translation="MRKPPSPKPPSTPPPNPPILGDTRLPRPVREVLKKTTTLPQDKA
                     EPILKQLKKIIVS"
     gene            complement(2519..3064)
                     /locus_tag="PKZ53_08635"
     CDS             complement(2519..3064)
                     /locus_tag="PKZ53_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40542.1"
                     /translation="MKGLELLLLSVLILLSGCGLTGAVLMARLYLRQSIEQLDKTAIN
                     RSGIYQFFVGLFNFILLMTVAIVLAQIPALRILALLILFAMTWLTFLGLTTRMRLIGL
                     DVMLLHKTPRASEIIQVLVGGAIVTIVAATPLLGICFAGIMLIQSFGTGLLWLVTRRK
                     HQPGESDLTAPIRPPETKVSG"
BASE COUNT          921 a          786 c          795 g          764 t
ORIGIN      
        1 gtggttcgcg atcagcttcg ccatatggac cgcaggaaat tgtttctgaa ggccgggtcg
       61 ctcggaaatt tacgcagcac tgtgagaaat tttactccga agtggagtca ctgctgccgc
      121 cagcctgtgc aatttacatt tgcggacctg gggaagccaa acttgaattc aataaacatc
      181 tggctcgaaa caagaacctt gccgaacaca tccgggatat tgaaccggct gatgagctaa
      241 cggaaacgca aatcgtggcc tgtgtgaaag agtatttcca aattgcctga aaatttggag
      301 tgctgcgact tgtcgccgct ttagtatttt ctgaaggtgt ggagtgctgc ggcttgacgc
      361 agctttggta ttttcgccac gggttcccac tcacgtctga acccgtggct ttgcgttttc
      421 aaactgtttc tgcggaatca cttcgcgcta gttacctgcc tgaacccaaa cgttcactct
      481 aaacgatgcc agttttgtgg acccgcttcc acaaaacaga acaaagcagc aaactaccgc
      541 acagcgagaa cagtaccagg cttccaatga gcacagcccc gagaagcacc aggtcattga
      601 cgtcaaaatg attccgaagc ttaaaaaagc gggtgttttc ccttaccaga caataagttg
      661 taaaactcac cagccctgac agaaaactat agatcacgcc aatctcagtg cttaacccga
      721 catattccag aaagttgggt tgtaattggc caacgattgg aagccgcttg tgcagggcat
      781 cccgaatcgc agccagcgtg aaaaaaagaa ccaatgtcca cactccaagc aatccggacc
      841 cgccacctga agaaatcagg agccagggga agcaaattgc gccaatgagg aaaaaatccc
      901 agaattgtac ttcaaaaggg tggagctttt ttgattggga tttctgactg gccagtgaaa
      961 caggctgaca aactccggca caccggttac aaatcccaga atctggattg aaacagtcag
     1021 tttggtgaca gatttcacaa cgaacaggtg gtttctcaga ttttacagtg agcttgttca
     1081 tcggctacac cttccaaaat cagatcgttt acactggttg cggttggccc gttttcgtcg
     1141 cttccaaaac agagaactaa agagcaaacc gctcaaagtt gaagcaaagg tcagcattcc
     1201 aatgaccagt cccgctccaa tcggcaggtg ttcaattcga aaggcatggg cgttcaatgt
     1261 aaaaagtgac cagatatctc gaacaatgca aaaggtcaca aaactcacca gcccggagca
     1321 aaagccatag gtgaggccca tggcgacaca ccgtagctca aacttcaata aatcggcggg
     1381 gctttgacca aactggtgaa tcaggtggca gatgtcgtgc attaaaaata tcagagttac
     1441 cagtagagca accgtccagg cgccgatagc tggttcgtgt gttccaactc cgattgagaa
     1501 ccagggaaag caaagcaggt agagcgtgac gtaatcccag aagctgggaa aatcaacagt
     1561 atgaacgtct ttctgatcaa ggttggggtt ggtcaggtca atattgaacc acttcatctt
     1621 tccgtactct ccagaaaacg gcgtgcagat tccgtaaatt gttcaactgt cagcgattgc
     1681 cggagatgat tttcaatcgt caattccgaa taattgatcc aaacctgcca gccagtcaga
     1741 tacacatccg ttaaaaagcc agccatctcg ccagcatcag gtgatgcgaa gtcaaactgg
     1801 aaatgaagcc gaggggtgaa cttttcaacc aggacttcat ctctggcata aaaacaatac
     1861 cactcgaaaa tcatttgatc gcgaaattgt ctgaggagta attccatcaa ttggattgac
     1921 ggagttgaaa cattcttgag ttctggtggg aaatactgtg attgcagcgt gccaatcgta
     1981 atactggtaa ctcctgaaaa atgaaagaaa taatagtctg aaaaagtgat ggtgccatcg
     2041 cctggttcca gacaaaattc gatggcagcc atcacttttt gggtgttgaa caccggtaaa
     2101 agaaaacaca gccgggcgtt gccttcgatg actgaattca actcattacg ccgagccagg
     2161 aacaattcaa tcatgggaat cctgatggtg gattaactca caataatttt cttgagctgt
     2221 ttcagaattg gttccgcctt atcctgagga agtgtcgtcg tctttttcag gacttcgcga
     2281 actggtcgtg gaagtcgtgt gtctcccaaa atcggcgggt tcggaggcgg tgtggacggt
     2341 ggtttgggtg atggtggttt gcgcataaat cctccttttt gatagggctg ggagaaccag
     2401 ggctcagggt ttggggctga agactcgcaa gctcggggct gaagaaaatg ggttcaatac
     2461 cctacgcccg aagtcttcat cccgccttct tcagccctca gccccaagcc cgaagtcttc
     2521 agcctgagac cttcgtttct ggtggacgaa ttggggccgt aagatcagat tcgccaggct
     2581 ggtgtttgcg tcgagtgaca agccagagta agccggtccc aaaactctgt atcagcatga
     2641 tgcctgcaaa gcatattcct aaaagtggag ttgcggcaac aatggtgacg atggcgcctc
     2701 caaccagcac ctggatgatc tcagaagcgc gtggagtttt gtgcaagagc atcacgtcca
     2761 ggccgatcag gcgcatccgg gtagtcagtc caaggaaggt cagccacgtc atggcgaaaa
     2821 ggatcaaaag cgccagaatc cgcaaggctg gaatttgagc caaaacgatg gctacggtca
     2881 tcaacaaaat aaaattaaac aggccgacaa aaaattgata tatccctgac cgattgattg
     2941 cggttttgtc gagttgttca atgctttgac ggaggtacag tcgtgccatc aagaccgcgc
     3001 cggtgagccc acacccggaa agcaggatca acactgacaa tagaagcaat tcaagtcctt
     3061 tcatgatggt tcctttccct taaaccccga accccaaatc ctgaccagat gaccaactga
     3121 caggtgacaa ggtgacaagg tgacagaggg tttttttatc cttcatcctt cccaaaaacc
     3181 caaaaaccct gaaccctgaa cccttatggt tttagtttga ctttcttttc ttgagtgcga
     3241 tcaacaggct gtttcccaga acggcc
//
LOCUS       DAOQDO010000462         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22618_length_3264_cov_2.962917, whole genome shotgun sequence.
ACCESSION   DAOQDO010000462 DAOQDO010000000
VERSION     DAOQDO010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22618_length_3264_cov_2.962917"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..638)
                     /locus_tag="PKZ53_08640"
     CDS             complement(129..638)
                     /locus_tag="PKZ53_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HNJ40543.1"
                     /translation="MERDTFWQLIDTVRTEANNDETQFEPILCSYLEELEPSDLIEFD
                     YHLRKLVTQAYLNQLWGAAYIINGGASDDGFYYFRAWLVMQGKTVYEKALKDPDSLVE
                     VCDQINFDAEAEEVLYAAGNVYEQRTGEEFESIIDPDDTGVWPTDPDWEEDDLPHLFP
                     RLSARYKDD"
     gene            complement(648..1013)
                     /locus_tag="PKZ53_08645"
     CDS             complement(648..1013)
                     /locus_tag="PKZ53_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40544.1"
                     /translation="MGTFTCACGHVIRDTTYPTDGAGMLYWEKDQEAINVQVASAVRD
                     FLAVRASYQRDWWLKSFFSSDYPVDLDDADIIDDIYSKFAFEYGLSVYQCPQCQRIYV
                     QKRPFENRWIGYKTEFDTK"
     gene            complement(1099..1224)
                     /locus_tag="PKZ53_08650"
     CDS             complement(1099..1224)
                     /locus_tag="PKZ53_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40545.1"
                     /translation="MDCTEPVCVGLREATLPECAIGGVDKGEKDNQWTYVWSAAA"
     gene            1239..1844
                     /locus_tag="PKZ53_08655"
     CDS             1239..1844
                     /locus_tag="PKZ53_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNJ40546.1"
                     /translation="MRFKETEIPQFQKHQSIFVHIPKTSGISVSKSLFNCTSPHVPMI
                     DYEAIFSQPELDRMFKFAFVRNPWDRAVSTYSFLAGRTGIERDIAWADRVLANYAGFD
                     DFIQRWLNRRNIYSWGLFFPQFFFVCNSQQEIRVDFLGRFENLNADFEYIKHRLGISA
                     ELLHLNKSKSRTDYRDYYTEKTRRIVADVYREDVELFGYQF"
     gene            complement(1946..2632)
                     /locus_tag="PKZ53_08660"
     CDS             complement(1946..2632)
                     /locus_tag="PKZ53_08660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ40547.1"
                     /translation="MVDSVEKIQSDFNRIAQLTENDPPHPILYQAFLLRQVPKNCAQV
                     LDVGCGTGNFARLLATRAEQVTGIDLSPEMIRIAQAQQPVSENIEYHCGDFFEFPFET
                     ESFDCVVSLATLHHFELQSALTRMKTILKPGGRLIIQDLRADATLIERMLSLIALSVT
                     ASQNLWRTGRLRQKREIRAAWREHAQGERYLTMAEVGELCATLLPGAVCYRHLLWRYS
                     LVWTRPSTDQ"
     gene            complement(2704..3264)
                     /locus_tag="PKZ53_08665"
     CDS             complement(2704..3264)
                     /locus_tag="PKZ53_08665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNJ40548.1"
                     /translation="MRYVIVDLEATCWENIRRPDRMEIIEIGAVHLASAMGPDTDEFG
                     RFVRPIVEPTLSDFCTQLTSITQAEVDAAETFPTVFADFLDWIGPEPFVLCSWGAYDL
                     GQFRTDCQRHQVPFPESFERHLNLKKEFARVFGIRPCGMAKALAHAGLTLKGTHHRGI
                     DDARNIARLAELILPQLEQTGFIEGL"
BASE COUNT          814 a          833 c          770 g          847 t
ORIGIN      
        1 gctgcaaagc tgaagacatc gtggatgcca ttgccattac agcggttcag gcacaaacaa
       61 agagctgaaa atcgcttttc ctttgacaag gcgtgatttg gcgagtgatt gagtccgtca
      121 aggcggattt agtcatcttt atatcgcgca ctgagtcgag gaaacaggtg tggcaggtcg
      181 tcttcttccc aatcaggatc tgtcggccag actccggtat catcaggatc aattattgac
      241 tcaaattctt cgccagttct ttgctcatag acattgcctg cggcatacag aacttcctca
      301 gcctcagcgt caaaattgat ctggtcgcag acttcaacca gtgaatccgg atccttcagc
      361 gctttttcat acaccgtttt gccctgcatc accagccacg cccgaaagta gtaaaaccca
      421 tcatccgaag cgccaccgtt gatgatatag gccgcacccc agagctggtt gagatacgcc
      481 tgggtgacca atttcctgag atggtaatca aactcaatta aatcagatgg ttccagttct
      541 tccagatatg aacagagaat tggttcaaat tgggtttcat cgttattggc ttcggttcga
      601 accgtatcaa ttaactgcca gaaggtatct cgttccattt ttgttcctca ttttgtatca
      661 aactcggttt tgtacccaat ccaccgattt tcaaatggcc ttttttgcac atatattcgc
      721 tgacattgag gacactggta cacactcagg ccatactcaa acgcgaactt tgagtaaata
      781 tcgtcaatga tatctgcatc atccaggtca accggatagt cgctggaaaa gaaagacttc
      841 agccaccaat cccgctgata actggcacgc acggcgagaa agtcacgcac ggcacttgct
      901 acctgaacat ttatcgcttc ctggtctttt tcccagtaaa gcataccggc gccgtcagtt
      961 ggataggtcg tgtcacgaat cacatgacca catgcacagg taaacgttcc catatcacaa
     1021 tctttcaaga agaataccgc agcttgacgc cacttttggg tttgatattt ttacactgcc
     1081 ctgaatcgaa aagcggcgtc aagccgccgc actccacaca tatgtccatt gattatcctt
     1141 ctccccttta tcaacgcctc cgatggcgca ttctgggagc gttgcctccc gcaacccgac
     1201 gcatactggt tcagtgcagt ccatggcgtg aagggattgt gcgatttaaa gaaactgaga
     1261 tcccgcagtt ccaaaagcac cagtcaatct ttgttcatat tccgaagaca tcgggtattt
     1321 cggtgtcaaa gagccttttt aactgtactt ctccacatgt gccgatgatt gactacgaag
     1381 ccatcttcag ccagccagag cttgaccgga tgtttaaatt tgcctttgtc cgcaacccct
     1441 gggatcgtgc tgtttcaacc tatagtttcc tggctggacg caccggcatt gagcgcgaca
     1501 tcgcctgggc cgaccgggta ctggcaaact acgccgggtt cgacgatttt atccagcggt
     1561 ggctgaatcg aagaaatatc tattcgtggg gtttgttctt tccacaattc ttttttgtct
     1621 gtaactctca acaggagatt cgagttgatt tcctggggcg atttgaaaac ctgaatgccg
     1681 attttgagta catcaagcat cgcctgggga tttcagccga actgcttcat ctcaacaagt
     1741 cgaagtctcg cactgattac cgggattatt acactgagaa aaccagacga attgtcgctg
     1801 atgtgtatcg cgaagatgtt gaactgttcg gctatcaatt ttgaacttcc cttactgcag
     1861 cattttggcc gagtattgtt ggataacctg aatccttaaa taattcaatt aaaagactta
     1921 gcaggttgtt ggtgctaaac tgacattatt ggtcggtgga aggtctggtc cacaccagtg
     1981 aataccgcca aaggagatgc cggtaacaaa cggctcctgg caggagtgtg gcacatagtt
     2041 cgccgacctc agccatggtc aggtaccgtt ctccttgagc atgttcacgc caggcagccc
     2101 gaatttcgcg tttttggcgc aggcggcccg ttcgccacaa gttttggctg gcggtgacgc
     2161 tcagggcgat gagagatagc atccgttcaa tcaaagttgc atcagcgcgc aggtcctgga
     2221 taatgagcct gccacctggc ttgagaatcg ttttcattcg cgtcagcgct gattgaagct
     2281 caaaatggtg aagcgttgcg agtgacacta cgcaatcaaa agattcagtc tcaaaaggaa
     2341 attcaaagaa gtccccgcaa tggtattcaa tattttccga cactggttgt tgtgcttgag
     2401 caatccgaat catttcgggc gacagatcaa taccggtaac ctgttctgca cgtgttgcca
     2461 gtaaccgcgc aaaattacct gtcccgcatc cgacatcaag cacctgtgcg cagttcttcg
     2521 gcacctgtcg gagcaaaaat gcctgataca gaatcggatg cggaggatca ttttctgtga
     2581 gttgtgcgat tcggttgaag tctgattgta ttttttcgac agaatccacc ataaagagaa
     2641 gttaactctg gtactcaatt tccctgggct cacccaatca gccattttga aggcagaacc
     2701 gtatcatagc ccttcaataa aacctgtttg ttcgagttgc ggcagaatca gttcggccag
     2761 tctggcaata ttgcgcgcat cgtcaatccc acgatggtga gtgcctttga gcgtgagccc
     2821 cgcgtgagcc agagctttgg ccattccaca gggacggatt ccaaacaccc gtgcaaactc
     2881 ttttttgaga ttgaggtgtc gctcgaaact ttcagggaag ggcacctggt ggcgctggca
     2941 gtcagtgcga aattgaccca ggtcataggc gccccacgag cagagcacaa acggctctgg
     3001 cccaatccag tcgaggaaat cagcaaaaac cgttggaaat gtctctgccg catcaacctc
     3061 cgcctgagtg atggaggtga gttgggtgca aaagtcgctg agggttggtt cgacaatcgg
     3121 acgtacaaat cgcccgaatt catccgtatc aggccccatt gccgaagcca ggtgaaccgc
     3181 cccaatttca atgatttcca tccggtcagg ccggcggatg ttttcccagc aggtagcctc
     3241 aagatcaaca atcacgtagc gcat
//
LOCUS       DAOQDO010000463         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22633_length_3263_cov_3.212905, whole genome shotgun sequence.
ACCESSION   DAOQDO010000463 DAOQDO010000000
VERSION     DAOQDO010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22633_length_3263_cov_3.212905"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKZ53_08670"
     CDS             complement(<1..755)
                     /locus_tag="PKZ53_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40549.1"
                     /translation="MTPILLPGAHHPQNSSLTWPHTKSLSLVKIPMFKSRLFRSFSLL
                     SCLFFAGLPGIAGLVNGQVAQSPAPVPTTTTSPVPAVSAAATAQRRPPHPQQISARAP
                     WIVNVAHVINLSEVADIYRKNQGIEVSPADAIPTSEVQLLNISTGIVIDSKGHVLVQL
                     AHLNPNESSNNLTICTTNDETLSAKFVGLDGVTGLAVLKIEGLEIEPPAFLNDGPSQK
                     SIQLDQQVFVWLPTFGKTAPRMKARLRALAPPRG"
     gene            complement(872..1555)
                     /locus_tag="PKZ53_08675"
     CDS             complement(872..1555)
                     /locus_tag="PKZ53_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40550.1"
                     /translation="MLTCQQTRTSLEQADFVEESLQPEVVTHLSQCRDCQSLFEETKT
                     LRFWLRNQRRVATPQDFDHQLQTRLRAIKQERQKSGWFAWIPTPALAAVSAAVLLGCV
                     LIPQFLRQTPPVLTPSQVADAGSQPESLSPSPVRPEIAIPDATHTEEPVANLVSTAET
                     HRTVRRVSTSARHRVSEEPGGDVFLLVRDGSNTQHVVTVPRVIYGAQPILNTNAPRVE
                     VEADTQHVF"
     gene            complement(1623..2261)
                     /locus_tag="PKZ53_08680"
     CDS             complement(1623..2261)
                     /locus_tag="PKZ53_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ40551.1"
                     /translation="MSVALSYQIELNLERAISIESATDHELVAATKEGNEIAFQEIVR
                     RYRNPITNFIFRMLSDYDRALELTQETFLRIYTNAHRYEATFSFSTYIYRIATNLAIS
                     ELRQRKRRKMLSIFTPWKVNSESDDDEQMLDLPDTRLLQDEDLIDRERRLAVARAIKT
                     LPEKYRAALVLRDVEGISYDRIAEILEISEGTVKSRINRARNMLREKLAAYI"
     gene            complement(2591..2887)
                     /locus_tag="PKZ53_08685"
     CDS             complement(2591..2887)
                     /locus_tag="PKZ53_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40552.1"
                     /translation="MAGTPPTELFEPYERLIEINVVGTPLLVPENNAVLRCFQYINLE
                     GISYGDFCWNADCTNCQIWYQRPGEEKERSSLSCRMRVIEGMTVTRLSKHIRLF"
     gene            complement(3046..>3263)
                     /locus_tag="PKZ53_08690"
     CDS             complement(3046..>3263)
                     /locus_tag="PKZ53_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40553.1"
                     /translation="QGIGVGAGQYQSDQAALTDRYRRVRVLGTISIYYDDYDGLIRAG
                     VPVQQPYPLDRRRSNPFPGEYKGVRIP"
BASE COUNT          799 a          779 c          874 g          811 t
ORIGIN      
        1 ccccgaggtg gcgcaagcgc tcgtaatcgc gctttcatcc ggggggcagt cttgccaaac
       61 gttggaagcc agacaaacac ctgctggtca agttgaattg atttttggga cgggccgtcg
      121 ttcaaaaatg ctggcggctc aatctcaagg ccttctattt ttaagacggc cagtccggtt
      181 acaccatcca gcccaacaaa tttggcagaa agggtttcat cattggtggt acaaatcgtc
      241 aggttatttg acgactcgtt gggattcaaa tgggcgagtt gaaccaggac atgtcctttg
      301 gaatcaatca caattcctgt cgagatattc aacaactgga cttctgaggt tggtatcgca
      361 tcggctggac tgacttcaat accttgattt tttcgataaa tgtcagcaac ttcgctgagg
      421 ttgataacgt gtgccacatt gacgatccag ggggcacgcg cactgatttg ttgtgggtgc
      481 ggaggacgtc tttgggcggt cgctgccgcc gatacagccg gaacaggtga tgtggttgtg
      541 gttggaacag gggcaggtga ttgtgccacc tgcccattga ccagaccggc aatgccgggt
      601 agccctgcga agaagagaca actcagtaat gagaatgaac gaaacaacct ggatttgaac
      661 attggaattt taaccaaaga gagcgacttt gtgtgaggcc aggtcaacga tgagttctga
      721 ggatgatggg caccgggcag cagaattggg gtcatttcgc acctcccatc gaaatagttc
      781 accaccctcc ctggttggtt tccataacgg ctggaaatct attgactctg gttgcagcgt
      841 gattcgactt caaaccaacg gctggtcctc attaaaagac gtgttgggta tcggcttcga
      901 cttcaacacg tggggcattg gtattgagaa ttggttgggc gccgtagatc acccgtggca
      961 ctgtgaccac atgttgagta ttgcttccat cccgtaccag gagaaaaaca tcccccccag
     1021 gctcttcaga cacgcggtgc cgcgctgaag tcgaaacgcg gcgaaccgtt cggtgtgttt
     1081 cagccgttga aaccagattg gcaactggtt cttcagtatg ggtagcgtct ggaattgcaa
     1141 tttctggtct cactggtgat ggtgacagag attcgggttg agagccggcg tcagcaacct
     1201 gcgacggagt taaaacgggt ggtgtctggc gcaaaaactg gggtatgagc acacaaccca
     1261 ataacactgc ggcactgact gcagccagtg ctggggtcgg aatccaggca aaccatccgg
     1321 atttctgacg ttcctgcttg atagctctca accgggtttg gagctggtga tcaaaatctt
     1381 gtggggttgc gacgcgacgc tgatttcgca accagaaccg caaggttttg gtttcctcaa
     1441 acagcgactg gcagtcacga cactgtgaaa ggtgtgtaac cacctcgggt tgcagtgatt
     1501 cctcaacgaa atctgcctgc tccaaagaag tgcgagtttg ttgacaggtc aacatataag
     1561 tcctctaacc aaaagcgtga tcagtcaaag cctatgggca agatgagcag tcatcgttgc
     1621 gtttaaatat aggcggcgag cttttcacgg agcatattgc gagcacgatt gatccgcgat
     1681 ttcacagttc cttcagaaat ttcgagaatc tcagcaatcc gatcatagct gattccttca
     1741 acgtcacgga gaaccagagc cgcgcggtat ttttccggca gtgtcttaat ggcacgggcg
     1801 acggccaacc ggcgttcgcg atcaatcaaa tcttcatcct gaagcagtcg ggtatcgggc
     1861 agatcaagca tctgctcgtc atcgtcgctt tcagaattta ctttccaggg cgtaaaaatc
     1921 gaaagcattt tgcgacgttt acgctgccgg agttcactaa tcgccagatt ggtggcaatt
     1981 cggtaaatgt aggttgaaaa actgaaggtt gcttcatagc gatgggcgtt ggtatagatc
     2041 cgaagaaatg tttcctgagt taactcaagt gcccggtcat aatcgctgag cattcgaaaa
     2101 ataaaattgg tgatggggtt ccgataccgg cgcacaattt cctggaaagc aatctcgttg
     2161 ccttctttgg tggcggcgac taactcgtga tcggtggctg attcgattga aatagctcgt
     2221 tccaggttga gttcaatttg atagctcaaa gcaacactca tatcagttct gttccttgcc
     2281 ctcgcctcgt ctcccaaagg ggtgggaaac cggagtggtt gacccaggca gtgtgggtgg
     2341 gtgtacattc gcgacttttg gcaattattg tagttagatt cgatatccaa ctctcacgcc
     2401 tcaaggacat gaaggttccc aaaagaattg caaattgact cgccaacgac tgctagttga
     2461 gatcacgaac agaattgaat tgggttgacg gtgtcagatc agacacagtc agggaaggtt
     2521 gggattggtt tttggattgg tgaaggcagt gagttctcgc ctccagttcc tcagcctcaa
     2581 gcccagcccc ttaaaataac cgaatatgtt tgctcaatcg cgttaccgtc atcccctcga
     2641 ttacacgcat gcgacaggaa agtgaagacc gctctttttc ctcaccaggc cgctgatacc
     2701 agatctggca gttggtgcaa tcagcattcc agcaaaaatc accataggaa ataccttcca
     2761 ggttgatgta ttgaaaacag cgcaaaacag cgttgttttc tggaaccaac aggggcgttc
     2821 ccacaacgtt gatttcaatg aggcgctcat acggctcgaa gagttcggtc ggtggtgtac
     2881 ctgccatgcg agaaaaacct gagtggagat gactgggttg tgcgcaaatt gaatgggata
     2941 agaagacgat gcgtgtctct ggttttacca cttccctggc tgacacgcca aggtctcttt
     3001 tcagattcca ggctgttttt gggtaagtca cctgaacttc tcagcctatg ggattcgcac
     3061 acctttatat tcgcctggaa atgggtttga gcgccggcga tcaagcgggt acggttgctg
     3121 aaccggaacc ccggcccgga taagcccatc ataatcgtca taatagattg agatggttcc
     3181 caaaacacga acccggcgat accgatctgt cagggcagcc tgatccgatt gatactggcc
     3241 ggcgccgacc ccgatcccct ggg
//
LOCUS       DAOQDO010000464         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22656_length_3261_cov_2.366500, whole genome shotgun sequence.
ACCESSION   DAOQDO010000464 DAOQDO010000000
VERSION     DAOQDO010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22656_length_3261_cov_2.366500"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKZ53_08695"
     CDS             <1..208
                     /locus_tag="PKZ53_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40554.1"
                     /translation="AGPISGQVEVHLHFNAEASSHHQFDQNQPSQPVPTVERKSGLGG
                     DSTSDEETHSTVHLSADGLVSLRA"
     gene            292..483
                     /locus_tag="PKZ53_08700"
                     /pseudo
     CDS             292..483
                     /locus_tag="PKZ53_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
     gene            569..1810
                     /locus_tag="PKZ53_08705"
     CDS             569..1810
                     /locus_tag="PKZ53_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNJ40555.1"
                     /translation="MPYSFSFSTALSGLRAQSSALGVIGNNIANSNTTAFKSGSMSFS
                     DQFYDSLGLRFNAAGNPVQVGTGVGIAGVSTDYSQGTLKPSTSPVHMGIQGNGFFVVK
                     DTQGVQNYTRAGDFSLSREGYLVTPNGFRVQGYNATNGMVPDNAPLEALKIPIGQTLE
                     PNMTTEVTQKFNLNSADPVGTEFTASVVVYDSLGASHTLNLVYTKTSDLNYSVAGDLD
                     GTPVTSVTPAALTFDSDGNLTAPAAPGGLDVVPDASTLNGATLPNIAVNLYEADGTAN
                     VSNYAAESGVSSSNQDGYSAGTIAGLSVDPTGMVIAVFNNGQTRPAGQVALAVFNSED
                     GLSRQGGNQLGETISSGAASIGRPGTGGRGGVVGGALEESNVDLATEFTNLIVAQRGF
                     QANSRVVTTLNQTLQDLLQII"
     gene            2350..3210
                     /gene="motA"
                     /locus_tag="PKZ53_08710"
     CDS             2350..3210
                     /gene="motA"
                     /locus_tag="PKZ53_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNJ40556.1"
                     /translation="MLVLIGLIIVFASVIGGYLMVNGSMAVLLQPAELVVIIGACFGS
                     IVIGSPFKLLKLVIDQTPVVLKGSPYNRSAFSELLKMQYEVFINVKKSGWLALEGDIL
                     TPTSSPIFTKYPFFLHNHHALDFFCAAATLLVNGAAHPEEIELAMDTELETHHEENAI
                     APGILNKAADSLPGLGIVAAVLGIVVTMQHIDGEPAVIGHHVAAALVGTFLGLLLSYG
                     MVSPLATCIEKIAHDEAKYLQCIRAGLIAFANGAAPGTAVEFARRTVFSFDRPPGIEL
                     EKQLREIKPR"
BASE COUNT          776 a          860 c          821 g          804 t
ORIGIN      
        1 ggccgggccg atttccggtc aggttgaagt ccatcttcat ttcaacgccg aagccagttc
       61 acaccatcag tttgatcaaa accaaccttc tcaaccggtg ccgacggtcg agcgcaagtc
      121 cgggttggga ggtgattcca cctcagacga ggagactcac tcaacggtcc acctgagcgc
      181 tgatggactg gtgagtttac gggcttaatc tcagggacga cctctgttgt gtgccctgct
      241 ttgtttcact tcactatgga gaacaccacc atgtcaacac ccggaatttc gatgtcacct
      301 tttgccagca cgccgtcacc acaatccacc acaaccggtc aaaaagcacc tgatcgagac
      361 atgttcctga cgttgctggt tgcccagtta aaaaaccagg atccactgca acctcaagac
      421 ggaatgcaat ttgtcgcgca actagctcaa ttcagccagt tggaccagct catcaccata
      481 aatcaaaaac tggatgcgct cggcggctct ggcgcctgac ccaggctggc ggcccacctc
      541 ttcaaccatt cagatctatg gaggctctat gccctactca ttttcttttt cgactgcact
      601 ttccgggctg cgcgcccaat ccagcgcatt aggggtgatt ggaaataata ttgccaattc
      661 caatacaacg gcgtttaaaa gcggatcaat gagcttttca gatcaatttt atgattcgct
      721 ggggttgcgg tttaacgcgg cggggaaccc ggtccaggtt gggaccggag ttggcatcgc
      781 cggcgtctcg actgattaca gtcaaggaac gctcaaacca tcaacttcgc cggtacatat
      841 ggggattcaa ggaaacggct tttttgtggt taaggacacc cagggcgttc aaaactatac
      901 ccgggccggg gatttctcgt taagccgcga ggggtatctg gtcacaccaa acggattccg
      961 ggttcaaggg tacaacgcca ccaacgggat ggtgccggac aatgccccgc tcgaagcatt
     1021 gaaaattcca attggccaga cgcttgagcc aaatatgacc accgaggtca ctcaaaaatt
     1081 caatttaaac tcagccgacc cggttggcac cgaatttacc gccagcgtcg tggtctatga
     1141 ctcacttggc gcctcgcata ccttaaatct ggtctatacc aaaaccagtg acctcaacta
     1201 ttcagtggcc ggagatttag acggcacgcc ggtgaccagc gttaccccgg cggcattgac
     1261 atttgactct gacgggaatc tgacggcacc agccgcacca ggtggactcg atgtggtgcc
     1321 agacgcctcg acactcaatg gcgcgacgtt gccaaatatc gcggtaaacc tgtacgaagc
     1381 tgatggaacg gccaatgtgt caaattatgc ggctgaatcg ggcgtttcct cgtcaaacca
     1441 ggacgggtat tcggctggaa ccattgccgg attgtcggtt gatccaaccg gaatggtcat
     1501 cgccgtcttt aataatgggc agacccggcc agcgggacag gtggcgctgg cggtttttaa
     1561 ttcggaagac ggattgagcc gccagggtgg caatcaactg ggcgaaacca tcagttcggg
     1621 cgccgcttca atcggtcgcc ctggcacagg cggtcgaggc ggggtcgtgg gaggcgccct
     1681 ggaagaaagc aatgttgatc tggccaccga atttaccaat ttgattgttg cccagcgtgg
     1741 gttccaggcc aattcgcgag tggtgaccac actcaatcag acgctccagg atttgctcca
     1801 aattatttaa agtatgaggc ggcaagcccg ccaactcaga gccaaagaag acagagcgta
     1861 ttaagtacct tgtcaaaagt ttcctgagcg attgaatgtt ggaagtgttt ggtttttttc
     1921 gtgtatttcg tgtatttcgt ggttcaaact gtctggaaat tttcggtaag gtacttattt
     1981 taggatcagg tgctcagggg cagtggtcaa cgaaatacaa ttctttcaat aatttagcgc
     2041 ttctgtaaca cgaggggttc ttgaccaaat ggtacataga acccctcata tcttccaccg
     2101 taagcccgcc accctttcgc ctactttctt ccgcccattc ccccacaatg gcatattgct
     2161 tgcaaacata atcctccagg atcagagttc acgcttgagc catagaactc gttacctggt
     2221 aagactttat cctttcaaat gattgagtgt tgcacagtac ttttcttacg gcctgctcaa
     2281 gggtgaactc ccaaaatttc gatccagcac actcaggtgt gtattctgga aatagcacag
     2341 gacaggctta tgctggtact cattgggtta attattgtat ttgcaagcgt gatcggtggg
     2401 tatttgatgg tcaacggctc gatggccgtt ttgttacaac ctgctgagct ggtggtgatt
     2461 attggggcct gctttggttc gattgtgatt ggctcgccat tcaaattact caagctcgtg
     2521 attgaccaga ccccggtcgt actcaaggga tcaccctata accgaagcgc cttttccgaa
     2581 ctcttgaaga tgcaatatga ggtctttatc aatgtcaaaa aaagcggctg gctcgccctt
     2641 gaaggcgata ttttgacacc cacctcatct cctattttta ccaagtatcc gtttttcctt
     2701 cataatcacc atgcgctcga ctttttctgt gctgcggcca cgctgctggt caatggcgcg
     2761 gcccatccgg aagaaattga actggccatg gataccgaac tcgaaaccca ccacgaagaa
     2821 aatgccattg ccccaggtat tctcaacaaa gccgccgact cactgccggg gttgggaatc
     2881 gtggccgccg tgcttgggat cgtggtgacg atgcagcata ttgacgggga accagcagtg
     2941 attggccatc acgtggccgc cgcgctggtt ggtacatttc ttggactgtt gctgtcgtat
     3001 ggcatggtct ctccgctggc cacctgtatt gaaaaaattg cccacgatga agccaaatac
     3061 ctccaatgta tccgggccgg gctgattgcc tttgccaacg gtgccgcacc tggaacagcg
     3121 gttgagtttg cccgtcggac cgtgtttagt tttgatcgtc cgcctgggat tgagcttgaa
     3181 aaacaattgc gtgaaatcaa accccggtaa gtaccttacc gaaaatttcc agacattttc
     3241 accacggaaa ccacggaaaa a
//
LOCUS       DAOQDO010000465         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22664_length_3260_cov_3.203120, whole genome shotgun sequence.
ACCESSION   DAOQDO010000465 DAOQDO010000000
VERSION     DAOQDO010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22664_length_3260_cov_3.203120"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3260
                     /locus_tag="PKZ53_08715"
     CDS             <1..>3260
                     /locus_tag="PKZ53_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40557.1"
                     /translation="FRPFLQDHPIIKGRRINLLFEDSQGRLWVGASGAGLIMKDGNRW
                     TSYTNQQGLPGNTPMALFEAADGILWVLTETGIGQFHNSTLTTVSLPAVITGTVMRTF
                     LVEPSGTLWIGTDIGLFRLSNDEVQYLQLPPSPAGNQVQSLVRTPQGQLLAGTDQGVF
                     VVENGGFIPVQAINTFLKNEVLSLQFDRDQNLWIGNEGGGLVRMTPQGVVSSLTTQNG
                     LPNNKIWKVIEDREGSLWVGTNGGLIRLRNGKVKSYTMREGLSDDYIRAVFADRSGVL
                     WMGGNNGSGINQLQGEKFTHITTKESLSSNDVRAFYQDRTGGIWVGTNNGLNFWNGSS
                     WSTFTRDQGLSNNLINAIGEDSTGRLWVGTENGLCRLENSRFVPYTRANGLPNNFIRA
                     IFTDRTGQLWIGTYSGLARMAGDTFSVIPQAGLDAAQILSFYEDLDGTLWVGTNNGLR
                     RFKNSAWTIFTTQQGLFDNTTFQILPDQQGNLWMSCNRGIFSAPVTAFDEVERDPAKK
                     ISCQVLGKADGMPSFQCNGGTQPAGCRDQSGRLWFPTAKGVVVVDPAHLNRNEVPPPV
                     VINEVLFDRAGLDPTRPAELQAGQESFEFHYAALSLQVPELVKFRYQLVGFDHNWIDV
                     GTRRVAYYTNLPPGEFTFRVIACNNDGVWNDTGASFSFRLNPPFWKTRAAFFLYAVAI
                     LGLISAGVWLRLRTMQRRNAELAAKVAEQTAELQQTVAELQHAKQEIEAKSRETEAKN
                     RELDSKIEELIASQKRADRIFSALAEALPGTVLDEKYRLDEKIGAGGFGAVFRATHLG
                     LNRPIAVKIFRPSSGNDSAEAVERFRLEGVSASRVNHPNAIAVLDSGISSDGIAYLVM
                     ELLEGHSLADELKRNGRLSLARCARIVEPVCQVLAEAHRAGIIHRDIKPDNIFLHQSA
                     EGEIVKVVDFGIAKLSGENTAENFQNLTASGLIIGTPNYMSPERFKGVPLDGRADVYS
                     LGVMLYEMICGVFPYSNCGNEFMSLVMAHLQAEPTPLRTFLPDLPAEIEECLLKTLAK
                     DPEDRPGVAEFGQEFSFLVHQFGLEFETIEISAGSTLANLPTVGQPISTHPTETFIT"
BASE COUNT          748 a          832 c          907 g          773 t
ORIGIN      
        1 tttcgtccat ttctccagga tcaccccatc atcaaaggcc gccggatcaa tctgttgttt
       61 gaagattcac agggacggct ttgggtcggt gcttccggcg ctggattgat catgaaggat
      121 gggaacaggt ggacatccta caccaaccag cagggattgc ccggcaacac gcccatggct
      181 ttgtttgaag cagcggatgg aatattgtgg gtgttgaccg agacgggcat cggacagttt
      241 cacaacagta cgttgacaac cgtttccctc ccagccgtca ttactggaac ggtgatgcgc
      301 acctttttgg tggaaccatc cggcacactc tggatcggca ctgacatcgg actgttccgt
      361 ctttcaaacg atgaagtcca ataccttcaa cttccaccca gcccggcggg caatcaggtg
      421 cagagcctcg ttcgaactcc gcaaggccag ttgcttgccg gaactgatca aggcgtgttt
      481 gtggttgaaa atgggggatt tatcccagtt caagctatta acacgttcct gaaaaatgaa
      541 gttctttcct tacagtttga ccgtgaccag aatctctgga ttggaaacga aggcggcggt
      601 ctggttcgaa tgacgccgca gggagtggtt tccagcctga cgacccaaaa cgggttgcca
      661 aacaataaaa tctggaaagt tattgaagat cgggaaggca gcctgtgggt cgggaccaac
      721 ggtggactca tccgattgcg caatggaaaa gtcaaaagct acaccatgcg cgaggggctc
      781 agtgatgact atattcgagc cgtatttgcc gaccgctctg gcgttttatg gatgggagga
      841 aacaatggca gcggaatcaa tcagttacaa ggcgagaaat ttacccacat tacaaccaaa
      901 gagagcctga gcagcaacga tgtgcgggca ttttatcaag accggactgg tggaatctgg
      961 gtcggcacca acaatggtct gaatttctgg aacgggtcga gttggtcaac ctttacgcgt
     1021 gaccagggac tgtccaataa cctgatcaat gccattgggg aagattcgac tggccggctc
     1081 tgggtcggaa cggaaaatgg tctgtgtcgg cttgagaata gtcggtttgt gccgtacacc
     1141 agggccaatg gcttgccgaa caactttatt cgcgccattt tcactgaccg aaccgggcag
     1201 ttgtggattg ggacgtacag cgggctggca cgaatggcgg gtgatacctt ttcggttatt
     1261 ccccaggctg gacttgatgc cgcccaaatt ctttcctttt atgaagatct ggacggtact
     1321 ctttgggttg gtaccaacaa cggcctgcgc cgattcaaaa acagcgcctg gacaatattt
     1381 accactcagc agggcttgtt tgataacacg acgtttcaga ttctacccga ccagcagggc
     1441 aatctgtgga tgagttgcaa tcggggaatt ttttcggccc cggtgaccgc ttttgatgag
     1501 gtcgagcgtg atccagccaa aaaaatctcc tgccaggtgc ttggaaaagc cgatggcatg
     1561 ccttcgtttc aatgtaatgg cggtactcag ccggctggct gtcgtgacca gtcgggtcgc
     1621 ttgtggtttc caacagcgaa aggcgtggtc gtggttgatc cggcacatct caaccgcaat
     1681 gaagtgccgc cgccagtggt catcaatgag gtgctctttg atcgggcggg gcttgaccca
     1741 acccggcccg ctgagttgca ggccgggcag gaatcatttg aatttcacta tgcggcactc
     1801 agcttacagg tgccggagtt ggtcaagttt cgctatcaac tggttgggtt cgaccacaac
     1861 tggattgatg ttggcacgcg ccgggtggcc tattacacca acttgccacc gggtgaattt
     1921 accttccggg tgattgcctg taacaacgat ggtgtctgga acgacacggg ggcgagcttc
     1981 tcgtttcgac tgaacccgcc attttggaaa acccgagctg ctttttttct ctatgctgtc
     2041 gccattcttg ggttgatatc tgccggggtc tggcttcggc tgcgaacgat gcaacgacgc
     2101 aatgcggaac ttgcggccaa agtcgccgag caaacggctg aactccagca aactgtggcc
     2161 gaattgcagc acgccaaaca ggaaatcgaa gccaaaagcc gtgaaaccga agccaaaaac
     2221 cgcgaactcg attcaaaaat cgaagagctg attgcctccc agaaacgagc tgatcggatt
     2281 ttctctgcgc tggccgaagc gctgccggga accgttttag atgagaaata ccgccttgat
     2341 gaaaaaattg gcgctggtgg gtttggggct gtgtttcggg cgactcattt ggggctcaat
     2401 cgtccaatcg cggtgaaaat ctttcgcccc tcttctggga acgattccgc cgaagctgtc
     2461 gaacgcttcc gccttgaagg ggtgtcggca tcacgggtca accaccccaa cgccattgcc
     2521 gtgctcgact ccgggatttc aagcgacggg attgcctatc tggtcatgga attgctcgaa
     2581 ggtcattcac tggcggacga attgaaacgc aatgggcgac tttccttggc ccgttgcgcc
     2641 cggattgttg agcctgtgtg tcaggtcctg gctgaagccc atcgcgccgg gatcattcac
     2701 cgcgacatca aaccggacaa catctttctg caccagtcgg cggaaggtga aattgtcaaa
     2761 gtggttgatt ttgggattgc caaactgtca ggtgaaaaca cggctgagaa ttttcagaat
     2821 ctgaccgcgt cggggctcat cattggtacc cccaattata tgtcgccaga gcggtttaag
     2881 ggcgtgccac tcgatggtcg ggctgatgtc tacagcctgg gggttatgct ctatgaaatg
     2941 atatgtggcg tgtttccata ttcgaactgt ggaaacgaat ttatgtcgct ggtgatggca
     3001 cacctgcaag ccgaaccaac gccgttacga acgtttctgc ctgatctccc ggctgaaatt
     3061 gaggaatgtc ttttgaagac cctggccaaa gacccagaag accggcccgg cgtggccgag
     3121 tttggtcagg agttctcctt cctggtccac cagttcggac tggaatttga gaccattgag
     3181 atttcggctg gctcaaccct tgcgaatcta ccaaccgtcg gccagccaat ttcgacccat
     3241 ccaactgaaa ccttcatcac
//
LOCUS       DAOQDO010000466         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22681_length_3259_cov_3.359863, whole genome shotgun sequence.
ACCESSION   DAOQDO010000466 DAOQDO010000000
VERSION     DAOQDO010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22681_length_3259_cov_3.359863"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3112)
                     /locus_tag="PKZ53_08720"
     CDS             complement(<1..3112)
                     /locus_tag="PKZ53_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ40558.1"
                     /translation="MPTLDWKFFGIVLKLSDDTYLSKALLFPEFSCLTNQPAKAHAAL
                     SANVAQVVENLSLGTVHQRQLADPIEPGRWNLTLHPPKGVFWREPLNLEFSGVTWQHG
                     DAQLAYLPQLEETVIVSGTGDLNEEILQQVRQHILRTESNLSLYKLLLLQQAEEVQTS
                     YLTRQITLLRPKQVMSKKDEDSEKKKSVLEQVATNLNCGFLPVAYEVDDLVEMLAAAL
                     TGRVQRSVLLVGASGTGKTAIFHELVRTRSKHHLGHTPFWATSGSRLVAGMTGFGMWQ
                     ERCVDIWRETTSTKALLHLGNLVELMEVGKSASNEQGIASFFRSYLARGDMLVVAECT
                     PEQITVIEREDPHLLQVFHRIDIAEPSFEVGQKILRRVASDLVPGREVVLDDLGIETL
                     DRLHRRYATYSAYPGRPLRFLKNLIQDAIEGSTLTNTEVTQAFSRETGLPLFLLDESI
                     PLDLENVHTWFSQQVIGQPQAVDLVVDLLAAIKAGVTRPNRPLSSFLFVGPTGVGKTE
                     MAKGLAEFLFRDRARMTRFDMSEFATPAGILRLIGGLGLAEGLLTAKVREQPFSVILF
                     DEVEKAHPMFFDLLLQVIGEGRLTDASGRLADFRNAVVIMTSNLGAESYRRNTIGFGD
                     DRQPEAVVRHFVEAVRNYLRPEMFNRIDRIVPFGPLSPETIVAVARREIDLMQRREGI
                     KFRNLHLDVSSDVPGFLAVKGYDVVYGARPLKRCLERELLVPLSTALNRYAQALPLRV
                     TVSVAGEALSVIVQPREDGSTKASAVRLAESRLRDLADRSSEFVRKVLKLTHSIAFIN
                     LRNEKYRVERSIQQQQKKKHAPALGALLARLPILKQLTIRGVELEEKSRALENQILLD
                     FYQHTLKDLKPYQLELEQLAEMRLRLLIDIFALTFEEPDHVIVAVYGKNPEFIRELVS
                     SYLEVASRHQWTTKLIQYAQPKEKTKDPLSLEGTVVEKPDEFFKNPPAVLPGLAIQMD
                     GPQAGLLMLAESGLHMFEKNAKTIGCLVETSSASLAAYEPIIWAELTEKLDAAEKRRI
                     YSVEEGK"
BASE COUNT          821 a          865 c          811 g          762 t
ORIGIN      
        1 ccttcccttc ctcaaccgaa tagatccgcc gtttttcagc cgcatccagt ttttcagtga
       61 gttcagccca gataatcggt tcataagcgg ccagactggc cgaactggtt tcaaccaggc
      121 agccaatcgt tttggcattt ttttcgaaca tgtggaggcc actttcagcc agcatcaaca
      181 acccggcctg tgggccatcc atttgaatgg ccagccctgg cagaaccgcc ggaggatttt
      241 tgaaaaattc atctggtttt tcaaccaccg ttccttcgag cgagagggga tctttggttt
      301 tttccttagg ctgggcatac tggatcaatt tcgttgtcca ctggtgtcgg gaagcaactt
      361 ccagatagct cgaaaccaat tcgcgaatga actccgggtt ttttccatag accgcaacaa
      421 tcacgtggtc aggttcttcg aaggtcaacg caaatatatc aatcagcagc ctcagtcgca
      481 tttcggcaag ctgttccagt tcaagctgat agggtttgag gtctttcaaa gtatgctggt
      541 aaaaatcaag caggatctga ttttcgagag cccgtgattt ttcctccagt tcaactcccc
      601 ggatggtcaa ttgttttaaa atcggcaacc gggccagcaa tgctccaagg gctggggcat
      661 gttttttctt ttgttgctgc tggatactgc gttcaacccg gtatttttca tttcgaagat
      721 tgataaaggc aatgctatgt gtgagtttca gtactttacg cacaaattcg gagctgcggt
      781 cggccaggtc ccgtagccga ctttcagcca gtcgaacggc gctggctttg gttgacccat
      841 cttctcgcgg ctgaactatc actgaaagcg cttcgccagc aactgaaacc gtaaccctga
      901 gcggaagcgc ctgggcatat cggttgagag ccgtactgag cggcaccagc aattcacgtt
      961 cgagacaccg tttgagcgga cgagcaccgt acacgacatc ataccctttg acagccagaa
     1021 aacctggaac gtccgaactg acatccagat gcagattgcg aaatttgatc ccttcacggc
     1081 gctgcattaa atcaatttca cgcctggcga cagcgacgat ggtttcaggt gaaagtggcc
     1141 caaacggaac aatgcgatca atgcgattaa acatctctgg ccggagataa ttccgcacgg
     1201 cttcgacaaa gtgccggacg acggcctccg gctggcgatc atcgccaaac ccaatggtat
     1261 ttcgacgata ggattcagca cctaaatttg aagtcataat caccacggca ttgcgaaaat
     1321 cggctaaccg tccgctggca tcagtcaggc ggccttcacc aattacctgc agcagcagat
     1381 caaaaaacat cgggtgggct ttttcaactt cgtcaaacaa aatcaccgaa aagggctgtt
     1441 cgcgcacttt ggcagtcagc aatccttcgg ccagccccaa accgccgatt aagcgcagga
     1501 ttccagcggg tgtggcaaat tcactcatat caaagcgggt catccgggct cgatcacgaa
     1561 acagaaattc tgccagcccc ttggccattt cagtcttccc gacacctgtc ggcccaacaa
     1621 acagaaatga tgaaagcggt cggtttggtc gcgttacacc agccttaatt gccgccagaa
     1681 ggtcaaccac cagatccact gcctggggtt gcccgatgac ctgttgactg aaccaggtgt
     1741 gaacgttttc taaatccagc ggaatggatt cgtcgagcaa aaagagcggc agacccgttt
     1801 cacgtgaaaa tgcctgagtg acttccgtgt tggtcagggt tgagccttca atggcatcct
     1861 ggatcaaatt cttcaagaag cggagtggtc gtccaggata ggccgaatac gttgcatatc
     1921 ggcgatgcag ccgatccagg gtctcaatcc ccaggtcatc cagcacaacc tcgcgccctg
     1981 gaaccaaatc tgaagccacc cggcgcagga ttttctggcc aacttcaaac gatggctctg
     2041 caatgtcaat tcgatgaaac acctgaagca agtgtgggtc ttcgcgttcg atcacagtga
     2101 tttgctccgg agtgcattcg gcaaccacaa gcatatcgcc ccgagccaga tacgaacgga
     2161 agaaactggc aattccctgt tcgttactgg cagatttccc gacctccatc aattcaacca
     2221 ggtttcccag atggagcagc gctttggtac tcgttgtttc gcgccagatg tcaacacagc
     2281 gctcctgcca catcccaaac ccggtcattc cagccactaa tcgagacccc gaagttgccc
     2341 aaaacggagt atgtccaaga tgatgtttgc tccgggtgcg aaccagttcg tgaaaaatag
     2401 ccgtctttcc agtgccggaa gcaccaacca gcaatacact tcgctggact cgtccggtta
     2461 atgcggcggc aagcatttca accagatcat ccacctcata ggcaaccggc aaaaaaccac
     2521 agttgaggtt ggtggcaacc tgctccagca cagacttctt tttctctgaa tcttcgtcct
     2581 ttttggacat cacctgcttt ggccgcagta aagtaatctg tcgggtcagg tagctggttt
     2641 gaacttcctc agcctgttgc agcagcagta atttgtacag cgagaggtta gattctgttc
     2701 gcaagatatg ctgccggact tgctgaagga tttcttcgtt taaatctcca gtcccgctga
     2761 caatgactgt ttcttcaagt tgtggtaaat acgccagttg ggcgtcaccg tgctgccagg
     2821 ttacaccaga aaattccagg ttgagcggct cgcgccaaaa cacgcctttg ggggggtgca
     2881 gggtgagatt ccaccgacct ggttcaatgg ggtcagcaag ttggcgctgg tgaacggttc
     2941 ccagtgacag gttttccacc acctgagcaa catttgccga caacgcagcg tgggcttttg
     3001 caggctggtt tgtcaggcac gaaaattctg gaaacaacag tgcctttgag agataggtgt
     3061 catcgctgag cttgagaact attccgaaaa atttccagtc gagagttggc atggttttag
     3121 ggtctgggag aaagacaact tttagggacg aaagggacga aaaggacgaa aaggacgaaa
     3181 aggacgaaaa ggacgaaaaa gactaaagtt cgaaaacccg gaacccggaa cccggaaccc
     3241 ggaacccgga acccggaac
//
LOCUS       DAOQDO010000467         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22688_length_3259_cov_2.634831, whole genome shotgun sequence.
ACCESSION   DAOQDO010000467 DAOQDO010000000
VERSION     DAOQDO010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22688_length_3259_cov_2.634831"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2527)
                     /locus_tag="PKZ53_08725"
     CDS             complement(<1..2527)
                     /locus_tag="PKZ53_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40559.1"
                     /translation="MYQSQDKVRNILLIDEQPAARELVKAQFARASLPYEWIEAESLK
                     HIETARSRQVHLILTEVNFQGFTWREAQIMLETWFPHQPVIIVTSQDFEDLALQATDG
                     LAFDYVLKCSLRRLPLVIRQATGFLTTSMEIPLVQYRYLFQHNPQPMWVYDLHTLKFL
                     DVNESALRVYGYSREEFSRMTILDIRPPEDIDAVKSSVASLPQGLGIPKVWRHLTKDR
                     RQIEVEILSHGLEFNGSNARLVLVTDVTARRQAEAKLQQSEARFSKFFHSNPVGCFIT
                     TYPEGRILDANRQILSLLGFELEEVLNQTTLGLNMWVVPETRHQMLHKLELQQTPIHI
                     EAQVRTKTGEIKDTLSSVELIEIDGQPAMLSMLYDLTQLKQGQIALIESERHLAEAQR
                     LAKLGNWSREIKSDRLFWSKELYHIFELDERTFPATFEAFLALVHPDDRQAVTGFLAQ
                     ALETGNPFEHTYRILTKTGETKVILEHGEAERDQAGTVCRLYGYIQDITERTRAEAER
                     LELSTRLTHTLDTIAEGFYTLDAELRLTYVNREFERITRMNRDQVIGHKILELFPEIK
                     ATELWPAYVQVLETRKTKMLELYYAPLDVWVELRIYAVDTGLSVYFWDITEKRKTEAI
                     VKQIEEQLRQSQKMQAVGQLAGGIAHDFNNLLTAITGYTVLTLQRLHPADPLCKNLNE
                     IAKAADRAAGLTRQLLTFSRKQVMQFSVINLNVLVADMERMFHRLIGEHIQLRTRLFP
                     DLWPVKADQSQIEQVLMNLVVNARDAMPEGGSLTIETANVVLDRAYADHHFNVQPGEY
                     VLMAVSDTGCGMDAETRARVFEPFFTTKSRERGTGLGLPTVYGI"
     gene            2880..>3259
                     /locus_tag="PKZ53_08730"
     CDS             2880..>3259
                     /locus_tag="PKZ53_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ40560.1"
                     /translation="MEPRFTPLTSTHAITILETLQAIDIGFGNSPRPVMVLRLTSLQP
                     FDFRFLYTNRTVTELTGFEREVLEHHSLSAIFPPQLNQLQFEKLARTINSHFQDTIDL
                     ICIALDGTEHFVKATISPWRNTAE"
BASE COUNT          781 a          848 c          792 g          838 t
ORIGIN      
        1 taatgccata gacagtcggt agcccaaggc ccgtgcctct ctcgcgagat ttggtggtaa
       61 aaaaaggttc aaagacgcgc gccctggttt cagcatccat accgcacccg gtatcggaaa
      121 cggccatcaa cacatattcc cctggttgca cattaaaatg gtggtccgca taggctcgat
      181 ccagaaccac attggcggtt tcaatcgtca ggcttccacc ttcaggcatg gcatcgcggg
      241 cgttgaccac caggttcatc agaacctgtt caatctgact ttgatccgct ttgactggcc
      301 atagatcagg gaagagccgg gtacgtaact ggatatgttc cccaatcagc cggtgaaaca
      361 tccgctccat atcggccacc aggacgttga ggtttatcac gctaaactgc atgacctgtt
      421 ttcgactaaa ggtcagaagc tggcgggtca acccggcggc ccgatcagcc gcctttgcaa
      481 tctcattcag atttttacac agcgggtcag ccggatgcag acgctggagg gtaagcacgg
      541 tatagcccgt gatagcggtc agcagattgt taaagtcatg ggcaatgccc ccggcaagct
      601 gcccaaccgc ctgcattttt tgggattgcc ggagctgttc ttcaatttgt ttgacaatcg
      661 cttcggtttt ccgtttctcg gtaatatccc aaaaatagac gctgagtccg gtatcaaccg
      721 catagattcg cagttcaacc cagacatcca gcggcgcata atacagttcc agcattttgg
      781 tttttctggt ttccaaaacc tgcacatacg ctggccacaa ctcagtcgct ttgatctcag
      841 gaaacaattc cagaattttg tgcccgatga cctgatcccg gttcatccgg gtaatgcgtt
      901 caaactcccg attgacgtag gtcagccgta actcagcatc aagggtataa aacccttccg
      961 caatcgtgtc gagggtgtgg gtcaagcggg ttgagagttc gagtcgttca gcttcagccc
     1021 gggttcgttc ggtgatgtcc tggatgtatc catagagccg acacaccgtt ccagcttgat
     1081 ctcgctcagc ttcaccgtgt tccaggatga cttttgtctc tcccgttttt gtgagaatgc
     1141 gataggtatg ttcaaacgga ttgcccgttt cgagcgcctg agccaggaag ccggtgacgg
     1201 cctggcgatc atctggatga accagtgcca gaaaggcttc aaacgttgcc ggaaacgtcc
     1261 gctcatcaag ttcaaaaatg tggtacagct ctttggacca gaagagccgg tctgatttga
     1321 tttcccgact ccagtttccc agtttcgcca gacgttgggc ttcagccaga tgccgttcgc
     1381 tctcaatcag ggcaatctga ccctgtttga gttgagtgag gtcatagagc atggacaaca
     1441 tggctggttg cccatcaatt tcaatcagtt caaccgaaga cagggtgtct ttgatttccc
     1501 cggttttggt tcgaacctgg gcttcaatgt gaatcggagt ctgctgcagt tcgagtttgt
     1561 gaagcatctg gtgccgggtt tccggcacaa cccacatatt gagcccaagg gttgtctggt
     1621 tgagaacttc ttccagttca aagcccagca gcgagaggat ctggcgattg gcgtcgagaa
     1681 tgcgtccttc gggataggtg gtgataaagc atcccaccgg gttgctatga aagaatttgg
     1741 agaaccgggc ttcgctttgc tggagtttgg cctcggcctg ccgacgggca gtcacatcgg
     1801 tcaccagcac cagtcgggca tttgagccat taaactccag tccatgcgag agaatttcga
     1861 cctcaatttg ccgacggtct tttgtcagat gccgccaaac ttttggtatt ccaagacctt
     1921 gaggcaatga cgcaactgaa cttttgaccg cgtcaatatc ttcaggcggg cgaatatcca
     1981 gaatggtcat ccggctaaat tcttcccgac tatacccata gacccggagt gccgactcat
     2041 tcacatctaa aaacttcagg gtgtgcaggt catagaccca catcggttgt gggttatgtt
     2101 gaaacagata gcgatactgg accagcggaa tttccattga cgtcgtcaga aaccccgtgg
     2161 cctgacggat taccagcggg agtcggcgca gtgaacattt gaggacataa tcaaacgcca
     2221 atccatcggt tgcctgcagc gccagatctt caaagtcctg gctggtcaca atgatgaccg
     2281 gctgatgtgg aaaccaggtt tcgagcatga tctgtgcctc acgccaggta aacccttgaa
     2341 aattcacctc tgtcaaaatc aggtgaacct gccgtgagcg ggcggtttcg atgtgtttga
     2401 gcgactcagc ttcgatccac tcatagggta acgaagccct ggcaaactgg gccttgacca
     2461 gttcgcgggc ggcgggctgc tcatcaatca ataaaatgtt cctgacctta tcttgtgatt
     2521 gatacatagg gggcacaaca ggtcgtggca gggcaggtag aattggttgc ttgtgttaac
     2581 tatccgttag tttcgatatt accactaaag agctcaaagc aaaagatttt gttctgttgt
     2641 ccctgtccga tatccattct gtttaggact gaatatttta accgaagacg gttgatttca
     2701 gtctccccaa ctaaaatacc agttgaattt acagaaaaaa cagttgaaac cgaaagttag
     2761 ggatatttgt gaacggcgtt tgcattcaga attttaccca tggcatacct ttcctctgat
     2821 atgccattgc cttcaggaaa tcagccgtca ctgaaacatc ccacttgagg catggattta
     2881 tggagccccg attcactcca ctgacatcaa ctcacgcaat caccattctg gaaaccctcc
     2941 aggccattga tattggtttt gggaactccc ccagaccagt catggtgctc agattgacct
     3001 cactgcagcc atttgatttt cgattcctct ataccaatcg aacggtgact gaattgaccg
     3061 gctttgagcg cgaagtcctc gaacatcatt cactttcagc catttttcca ccgcaactca
     3121 atcaactcca gtttgaaaaa ctcgctcgta ccatcaactc tcattttcaa gataccattg
     3181 atctgatctg tattgcgttg gacggcactg agcatttcgt caaagccaca atttccccct
     3241 ggcgcaacac ggctgaatt
//
LOCUS       DAOQDO010000468         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22698_length_3258_cov_2.956915, whole genome shotgun sequence.
ACCESSION   DAOQDO010000468 DAOQDO010000000
VERSION     DAOQDO010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22698_length_3258_cov_2.956915"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1308)
                     /locus_tag="PKZ53_08735"
     CDS             complement(76..1308)
                     /locus_tag="PKZ53_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40561.1"
                     /translation="MFSAALWESIQQAFASLSANKLRTLLTLIGIVVGVTAVIAVVTV
                     IEGLNQKVAQTFASQGSNVFSVRKLPQIILSRDDLLRFQKRKDITEEDARFVTDRCHG
                     CDQVGWDFTTIGTVKFEHEKSENVIIRGISGNVPTIESLTLDEGRIFTEQEAQGSRNV
                     CIIGWDVVDNLFPSKDPLGKELKIAGRPYTVIGVSTRYGSLFGFSRDNFVMIPFSTFC
                     RVYGTGGSINIIIAATSTERIDQAQQEVRTLMRIRRQKSFRDEDDGFAIESSQVFLDL
                     YSDATRNIYLVSFVVSGISLIVGGIVIMNIMLVSVTERTREIGIRKAMGARRVDVLWQ
                     FLTEAVVISAIGGLIGIFCGFGLAYLISIYTAFPIAVKAWSAILGVTVSSLVGIVFGV
                     YPANRAAQLDPIEALRAE"
     gene            complement(1413..3083)
                     /gene="rpoD"
                     /locus_tag="PKZ53_08740"
     CDS             complement(1413..3083)
                     /gene="rpoD"
                     /locus_tag="PKZ53_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNJ40562.1"
                     /translation="MRSNLKDDYMNGEGDPTSGDNMVSSLDLEKFLAEIDSTTADDDD
                     FVWESDTEEVEEIEDAEDVTEALAAPQPDLSASEADRSLDAMTLYLREMSNVSLLTRE
                     DEVAIAKRIERGRRRLQKAHARSLIVAEALEELAEKLVHEQLSIRAVIDTSDSVAEDI
                     TLENEQKYLEETVEKFQQVTKLVVQIRKETVKIQEEQKNSKKMSRLLRKLSRRRIELS
                     RVIRTILFSNQMRREFTNRIQGVVRQILELEDQVTRAEKALASKKRVDENEMKKKIRL
                     AKKRLSEIEQRYVSTTVEIKRSLKQIISAEAEAEKARREMIEANLRLVVSIAKNYINR
                     SRGLQFADLIQEGNIGLMRAVEKFDYRRGYKSSTYATWWIRQAVTRAIADQGRTIRVP
                     VHMVETINKIVRTARLMVQELGREPTHEELAQRTDLPVVKVRQALKIAQEPISLETPI
                     GDDGESNLGSFIEDRNSLNPAEMIVGKGLREATLEVLNTLTPREAQILKMRFGLDNDG
                     QERTLEEVGRHFAVTRERIRQIEAKALRKLRHPSRSRKLKTFFDDDWR"
BASE COUNT          779 a          823 c          829 g          827 t
ORIGIN      
        1 caccaagaac caagcaccaa gaaccaagaa ccaagcacca agcaccaagc accaaaaact
       61 aagaacccaa ggaactcatt cggcccggag tgcttcaatt gggtcaagct gggcggcccg
      121 gttggctgga tagacaccaa acacaatacc gaccagcgat gaaaccgtga cgccgagaat
      181 cgctgaccag gctttgacgg cgattggaaa tgcggtgtag atcgaaatca gatacgccag
      241 tccaaacccg caaaagatgc cgattaagcc tccaatggct gaaatgacga ccgcctcggt
      301 cagaaattgc cataagacat caacccggcg ggcgcccatc gccttgcgga tcccaatttc
      361 gcgggtgcgc tcggtcactg agaccagcat gatgttcata atcacaatac caccgacaat
      421 gagcgagatt cccgagacga caaatgaaac caggtagata ttgcgagtcg catctgaata
      481 gagatcgaga aacacctgtg agctttcaat cgcaaagccg tcatcttcgt ctcgaaatga
      541 tttctgtcgc cgaatccgca tcagggtgcg gacttcctgc tgggcctggt caatgcgctc
      601 cgtactggtc gcggcaatga tgatattgat tgatccgccg gtgccataca cgcggcagaa
      661 ggttgagaag ggaatcatga cgaaattgtc acgtgaaaaa ccgaacagcg agccataccg
      721 ggtcgaaacg ccgatgacgg tataggggcg gcccgcaatt tttaactctt tgccgagcgg
      781 gtctttggac gggaagagat tatcaaccac gtcccagcca ataatgcaca cattgcgtga
      841 gccctgggct tcctgttcgg taaagattcg gccttcatca agcgtgagtg actcaatcgt
      901 aggcacattt cccgaaatgc ctcgaatgat gacattctct gatttttcat gttcaaactt
      961 aacggtgcca atggtggtga aatcccatcc gacctgatca cacccgtggc agcggtcggt
     1021 gacaaatcgg gcgtcttcct cagtgatatc cttgcgtttc tggaatcgta acaaatcatc
     1081 ccggctgaga ataatttgcg gaagttttcg gaccgaaaaa acatttgaac cctgactggc
     1141 aaaggtttgg gcgacttttt gattgagccc ttcgatgacg gtgaccacgg caatcaccgc
     1201 cgtcacgcca actacaattc caattaaggt gagcaacgtg cgaagtttat tggcggaaag
     1261 gctcgcaaaa gcttgctgga tggattccca aagcgcggcg gaaaacatgg ggtgttcaac
     1321 ggagagaagg ggaggctggt ggttggtatt ttcagatggg gcgacataca aaaatcaggc
     1381 cagactgtga agtctggcct gaatggttca agttatcgcc aatcgtcgtc aaagaaagtt
     1441 ttcaactttc ggctccgtga tgggtgacga agtttacgta acgctttggc ttcgatttgt
     1501 cggatgcgtt cgcgagtcac ggcgaaatga cgtccgactt cttcgagggt tcgctcctgc
     1561 ccgtcattat ccagaccaaa ccgcattttg aggatttggg cctcgcgtgg ggtcagggtg
     1621 ttgagaactt caagggtggc ttcccgcagt cccttgccaa cgatcatttc cgccgggttg
     1681 aggctgttgc ggtcctcgat aaagcttccc aggtttgatt cgccgtcatc accaattggc
     1741 gtttcaaggc tgattggctc ctgggcgatt ttgagtgcct ggcggacctt cacgactggc
     1801 aggtcggttc gctgggcgag ttcttcgtgc gttggttcgc ggccaagttc ctgaaccatc
     1861 aaccgtgcgg tgcgcacaat tttgttgatt gtttctacca tatggactgg aacgcggatg
     1921 gttcgtccct ggtcagcaat ggctcgggtc acagcctgac ggatccacca cgtggcataa
     1981 gtcgaggatt tatagccacg acggtagtca aatttttcaa ccgcccgcat caggccgata
     2041 ttgccttcct ggatcagatc ggcaaattgc agcccgcgtg accggttgat gtaatttttg
     2101 gcaatggaca caaccaggcg aaggttggct tcgatcattt cacggcgcgc tttttcagct
     2161 tcggcttcag ccgagatgat ttgtttgagc gagcgtttaa tctcaaccgt ggttgaaaca
     2221 tagcgttgct caatttccga aaggcgtttt ttcgccagcc ggattttttt cttcatctcg
     2281 ttttcatcaa ctcgtttctt cgaggcaagc gctttttcag cccgcgtaac ctgatcttcg
     2341 agttccagaa tctgacggac cacgccttga atgcgattgg taaactcccg acgcatctga
     2401 tttgaaaaca gaatcgtccg gatgactcgt gaaagttcaa tgcggcggcg ggacaatttc
     2461 cgcagcaacc ggctcatttt tttggaattc ttttgctctt cctggatctt gacggtttct
     2521 ttgcggattt ggaccaccag tttggtgacc tgttgaaact tttcaactgt ttcttcgaga
     2581 tatttttgtt cgttttcgag tgtgatgtct tccgccactg agtctgacgt gtcaatgaca
     2641 gcgcggatgc tcaattgttc gtggaccagt ttttcggcga gttcttcgag cgcttcggcc
     2701 acgatcaggg atcgggcatg cgctttttgg agtcgccggc gaccccgctc gattcgtttg
     2761 gcaatggcga cctcatcttc gcgagtcagc aatgaaacat tgctcatctc acgcagatag
     2821 agtgtcatcg catcaagtga ccggtctgct tcggaggctg aaagatcagg ttgtggggcc
     2881 gcaagggcct cggttacatc ttcggcatct tcaatttctt caacttcttc agtgtcactt
     2941 tcccaaacga aatcgtcatc atccgcagtg gttgaatcta tttctgctaa aaatttttcg
     3001 agatccaaac tggagaccat attgtcccca ctggttggat caccttcccc attcatataa
     3061 tcgtctttca agtttgacct cacgatgttt ttcccttccc aaaagccaaa agaccaaccg
     3121 gtaagggaag gtcttcagtt tttcggtaac aaccaacaac caactctgta gtaaagtgta
     3181 ctacagagac acgacagatt gacaaagcct tgggcaggct tttagtatac gcgtttattt
     3241 tagaaaaaac agccaaaa
//
LOCUS       DAOQDO010000469         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22709_length_3257_cov_2.858214, whole genome shotgun sequence.
ACCESSION   DAOQDO010000469 DAOQDO010000000
VERSION     DAOQDO010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22709_length_3257_cov_2.858214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PKZ53_08745"
     CDS             complement(<1..1092)
                     /locus_tag="PKZ53_08745"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNJ40563.1"
                     /translation="MSFSSFKPGTRNPEPGTRICTPILSPTLAKARQAIAQAQVHADW
                     IELRLDFLEDLDRDQPRLTVETLLAECSKPVIITFRPTEQGGHREISLDERLAFWHQV
                     LTLPDVHFDLEADFVPVLVEKLGAARLPWDKMIASWHEFDHTPENLPALIEQRFPRDA
                     ATIKLAVRTTSIRDLTALLATFQTLAAESRQRFILIGMGAPGAPTRILGPALGSQVTY
                     SAAPDGNLSAPGQFSAADLRQVYRIHRLSTTTKVTGLIGKPVGHSLSPHMHNAAFEAL
                     GLDWIYLPFEVQPEGTDLADFIRLFVHPQSRQFPFQPMGFSVTIPHKQAVIPLLDRLT
                     TTAEAVGAVNTLLIEGTRLIGHNTDVDGAM"
     gene            1264..2040
                     /locus_tag="PKZ53_08750"
     CDS             1264..2040
                     /locus_tag="PKZ53_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNJ40564.1"
                     /translation="MQTAPHATPKSVREQVSAEEWQIRVDLAACYRLVAHYRWDDLIF
                     THISARVPGADHHFLINPYGMMFDEITASSLVKVDINGNIVMETPYPINPAGFTIHSA
                     VHAAREDARCVLHVHSLNGVAVSAQKNGLLPLSQQSLFVLSSLGYHNYEGVALNEDEK
                     PRLVADLGEHKFLILRNHGLLTCAETIADAFLYMYLMESACTIQVRAQSGGGELIPVP
                     TPILQGIQAQAKVVTRSVGGALAWPGLLRKLDRIDPSFRE"
     gene            2307..2630
                     /locus_tag="PKZ53_08755"
     CDS             2307..2630
                     /locus_tag="PKZ53_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNJ40565.1"
                     /translation="MSAIAPTTVRYQSFREFYPFYLGEHSNLTCRRLHFIGSCLVLVL
                     LAITIAMRNPWLLLAVPVCGYGFAWVGHFFFEKNRPATFTYPVYSLIGDWVMFGQILT
                     GKIPF"
     gene            2642..>3257
                     /locus_tag="PKZ53_08760"
     CDS             2642..>3257
                     /locus_tag="PKZ53_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ40566.1"
                     /translation="MTYQTVRANGLDFAFLEAGKGPTVLLLHGFPDIAETWEYQIPEL
                     VQAGFRVVAPYLRGYPPTEIPQGGYSDRATLALDVKELILAVGNGEPVFLVGQDWGAA
                     IAYGVIAAFPELIRRAVVMAIPHPGMILQSLTNPEQVHRAFHWWFFQLPDLPEKSVVA
                     NNFAFIDYLWKYWSPGHDDADHISRIKTMLSTPGAISSTIGYYRA"
BASE COUNT          741 a          834 c          881 g          801 t
ORIGIN      
        1 catggcgcca tccacgtcgg tgttgtggcc aatcaaccgg gttccttcaa tcaataatgt
       61 attgaccgct ccgactgctt cggcggtggt tgtcagacga tcaagcaacg ggataactgc
      121 ctgcttgtgt ggaatcgtga cgctaaatcc catcggctga aatggaaact gacggctttg
      181 agggtgaacg aacaatcgga taaagtcagc cagatcagtg ccttcgggtt gtacttcaaa
      241 cggaagatag atccaatcga gccccaacgc ttcaaacgca gcgttgtgca tgtgtggtga
      301 cagtgaatgc ccaaccggtt ttccgatcaa tccagtgact ttggtggtgg tcgagagtcg
      361 gtggatacga tacacctgac gtaaatctgc cgctgaaaac tgacccggcg ccgatagatt
      421 cccgtctggc gcggcagaat aggttacctg actgccaagc gccggtccca aaatccgggt
      481 tggcgcaccg ggtgctccca tcccaatcaa aataaaccgt tgcctggatt cggctgccag
      541 tgtctgaaat gtagccagca atgccgtcag gtcacgaatt gaagtcgtgc gaaccgccag
      601 tttgatggtt gccgcgtcgc gtgggaaacg ctgttcaatc aatgccggaa gattctctgg
      661 ggtatggtca aattcgtgcc aggaagcgat cattttgtcc cacggaagcc ttgctgctcc
      721 cagtttttca accaggaccg gaacaaaatc ggcttcaaga tcaaaatgga catctgggag
      781 cgtcaaaacc tggtgccaga aggccagccg ttcgtcgaga gagatttcac ggtgaccacc
      841 ctgctctgtt gggcgaaacg tgatgatgac tggctttgag cattcggcca ggagggtttc
      901 aactgtcagg cgtggctgat ctcggtcaag gtcttccagg aaatcaagcc ggagttcaat
      961 ccagtcggca tgcacttgag cctgggcaat cgcctgtctg gccttggcaa gggttgggga
     1021 gagaatcggg gtgcagattc gggttccggg ttccgggttc cgggttccgg gtttaaaaga
     1081 cgaaaaggac ataaaggaca taaaggacaa tcgaggacat aaaggatata aatttgaaaa
     1141 cccggaaccc ggaacccgga acccgattag aatttcctga tggtttcagc cgctgatttt
     1201 gtagataata cgccacacac tccacgacat ccatttcacc acatccagga aggaaatttc
     1261 gctatgcaga ctgctccgca cgctactcca aaatccgttc gtgaacaggt atcagccgaa
     1321 gaatggcaga ttcgggttga tctggccgcc tgctaccgac tggtggccca ctatcgctgg
     1381 gatgacctca tctttacaca tatttccgcc cgtgttccag gggctgatca ccatttcctg
     1441 attaacccct atggcatgat gtttgacgaa atcaccgcct ccagcctggt caaagttgac
     1501 atcaatggaa atattgtgat ggagacgcct tatccgatta acccggcggg atttaccatt
     1561 catagcgctg ttcatgctgc ccgcgaagat gcccgatgtg ttttacacgt ccacagtttg
     1621 aatggcgttg ccgtgtctgc ccagaaaaac gggttgcttc cactctcgca acagtcactg
     1681 tttgtacttt caagccttgg ctatcacaac tatgaaggcg tggcgctcaa cgaagacgaa
     1741 aaaccacggc tggtggcgga tcttggggaa cataaatttc tgatcctgcg caaccacggc
     1801 ctgttgacct gtgctgaaac gattgcggat gcctttttat atatgtactt gatggaaagc
     1861 gcctgcacga ttcaggtccg tgcccagtca ggaggtgggg aattgattcc cgtgccgacg
     1921 ccgattttgc agggaatcca ggctcaggcc aaagttgtaa ccaggagtgt tggcggcgcc
     1981 ctggcctggc cagggctgct gcgcaaactt gaccggattg acccatcatt tcgggagtaa
     2041 gattttgggc tgaaaaaacc agggctgaaa aaaccagggc tgaaaaaacc agggctgaag
     2101 aagtcgggct gaagacattg ggctgaagaa tttgggctca gggtattgaa cccgttttct
     2161 tcagccccga gcttgcgagt cttcagccct cagctcttaa cccttagccc gaagtcttca
     2221 gccctcagct cttaaccctt agcccgaagt cttcagccct cagccctaag ctccagggtt
     2281 tctcagcccc agttcggagg tatgctatgt cagcgattgc acccacgacg gttcgctacc
     2341 agagttttcg agagttttat cctttttatt taggcgaaca ttcaaacctg acttgccggc
     2401 gactgcattt tattgggagt tgcctggtgc tggtcctgct cgccatcacg attgctatgc
     2461 ggaacccatg gttactgttg gcggtgccgg tgtgcggcta tgggtttgcc tgggttgggc
     2521 atttcttttt tgagaaaaac cgcccggcga cttttactta tccagtgtac agtttaattg
     2581 gagactgggt gatgtttggg cagattctaa ccggaaagat tcccttttga tgtgaggcgt
     2641 gatgacctat caaaccgttc gtgccaatgg cctcgacttt gcttttctgg aagctggaaa
     2701 agggccgacg gtgcttttgc tccacgggtt tccagacatt gccgaaacct gggaatatca
     2761 aatcccggaa ctggtacagg ctgggttccg agtcgtggcc ccgtatctgc gcggctatcc
     2821 gccaacagaa attcctcagg gtggatattc tgatcgggcg acactggctc tggacgtcaa
     2881 ggaattgatc ctggccgttg ggaatggtga accggtcttc ctggtgggtc aggattgggg
     2941 cgccgctatt gcctacggtg tgattgcggc gtttcccgaa ctcatccgcc gggcagtggt
     3001 gatggcgatt ccacacccag gcatgatttt gcaaagcctg accaatcccg aacaggtgca
     3061 tcgtgccttt cactggtggt tttttcagtt gcccgatttg cctgaaaaat cagttgtcgc
     3121 caacaatttt gcttttattg attacctgtg gaagtactgg tctccgggtc acgacgatgc
     3181 cgaccatatc agccggatta aaaccatgct ttcaaccccc ggcgcaatct cgtcaaccat
     3241 cggctattat cgggcaa
//
LOCUS       DAOQDO010000470         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22722_length_3256_cov_3.437051, whole genome shotgun sequence.
ACCESSION   DAOQDO010000470 DAOQDO010000000
VERSION     DAOQDO010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22722_length_3256_cov_3.437051"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..403
                     /locus_tag="PKZ53_08765"
     CDS             152..403
                     /locus_tag="PKZ53_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40567.1"
                     /translation="MNNHVAPSLTQSQIDALTFLIHHYKTGNLLATIQICHDFLACLP
                     DSTPEKQMFLNDITGLKKIVNLLAHVNTEEEPKASEAAQ"
     gene            539..>3256
                     /locus_tag="PKZ53_08770"
     CDS             539..>3256
                     /locus_tag="PKZ53_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNJ40568.1"
                     /translation="MRPLIQRVAIVDRGTAAVRAIRAIREYNEEHQTTIRSLALFTKT
                     DQRALFVEEADEGFLLGSDEWWSNGQVNSRYCDLEALEKALVASRADAVWTGWGWMSA
                     RPELADLCHKLGIAYLGVSGDLMRQMLTPEFAQHLAEQSGLTLDLEPAESLSDVRQLA
                     VQTLADADCTVWALGVLDTTFQFSETTGLVESPSPVLSPDQELMLCRTAVKMVKQSKA
                     TGLVSVKFVWKTTTGTVQNVSLQPGICASHPVTELRAGVDLVKLQIHLGRDGHLETHP
                     PALSPAHAMGVELVAIPEATDLPASSKIVESLKTVTGPGLRIDPGISEGNLLAQPFLP
                     SRWISLVTTSGHNRAETLSRLKRALLNSACVIHGYRCNKSQLLAMLNRPDVIDGHTTP
                     IPAATIAPLNPLFGKVALIQAAIEVYLADFSDEHAQFVTSAARGRPQVRGEAGLTVEL
                     GYQGEVYALTVYRLGDQNYRIDVDGQQIPVTVAWSNRHERRLIVAGQNFHTFPVVTQP
                     NFTIEVEGTEHHISREDFGVVRCPTPAVVVSVAVKEGDEVKTGDVVAVVESMKLEMPI
                     NAPYAGKVRKVLVLPNVQVDIGAPLIQFELSSHPAAGSAGERLRFSNLVPAAPDSLQA
                     RCHTNFEAFRSLLLGYDVAPAEVKRLMADRGQLCHQLPVTDPDLIGNEDEMLTIFVDL
                     CSLFQRLALTEDHEAAEALASQEYLLTYLRTFDTQGTELPQAFLDKLLRTLAHFGVYS
                     LRRTPELEEALLRLYKSHLKMDQHLTLMLSLLERRLANHEAMQPALSASFQWLLNRTI
                     EETLGSYPSLSELAREVRFRLYDEAIFEQARKRIYADVEEHLNYLEESPGAEDREVHI
                     QALANCPQPLAGLLAKRFGDMSLAMQQLILEVLTRRYYRIRKLENFQNLL"
BASE COUNT          752 a          935 c          840 g          729 t
ORIGIN      
        1 gtcgcgctca tccgcaatgt gttgagttga tgtctcagtc agtaccacct gtgtcagcga
       61 ctggaagtgc tgaaactgaa caagcccacc cgctgacaca ggtggtactg acaaattttc
      121 attcttcatt cattttagag aggatccagc catgaataac cacgtcgcac catcattaac
      181 ccaatcccaa atcgatgctt taaccttttt gatccaccat tacaaaaccg ggaacctgct
      241 ggccaccatt caaatttgtc acgattttct ggcctgcctg ccggactcaa ccccggaaaa
      301 acaaatgttt ttgaatgaca tcaccggttt gaaaaaaatt gtcaacctgc tggcccacgt
      361 caacactgaa gaagaaccca aagccagcga agctgcacag tagcgaaaga tctgtcagta
      421 ccacctgcct cagcgggtgg gccaggtgag tactatctgc ggtagcgatg ggcagttcag
      481 tcatttccta cccgctgacg caggtggtac tgacctgggg taaccctgga ggaaaattat
      541 gcgtcccctg attcagcgtg tcgccattgt ggaccgtgga acggctgccg ttcgggccat
      601 tcgtgcgatc cgggaatata atgaagagca ccagaccacg attcgctcac tggctctgtt
      661 taccaaaacc gatcaacgtg ccctgtttgt cgaagaagcc gacgagggct ttttgctggg
      721 ttcggatgaa tggtggtcaa acggtcaggt caacagccgg tattgtgatt tggaagccct
      781 cgaaaaagcc ctggtggcgt cacgtgccga tgccgtatgg accggatggg gctggatgtc
      841 ggcccgacca gaactggccg atctgtgtca caaacttgga attgcgtacc tgggggtctc
      901 tggcgacctg atgcgtcaga tgctgacgcc tgagtttgcc caacacctgg cggaacaatc
      961 tggcctcacg cttgaccttg aaccagccga atcactgtct gatgtgcgcc agcttgccgt
     1021 tcaaaccctg gctgacgctg attgcaccgt ttgggcactg ggcgttttag atacgacgtt
     1081 ccagttttca gaaacaaccg gtctggttga atcaccatcg ccagtgttgt cacctgatca
     1141 ggaactgatg ttgtgccgca cggcagtcaa aatggtcaaa caatcaaaag caactggact
     1201 ggtgagcgtc aagtttgtct ggaaaaccac gactggaacc gttcagaatg tcagcctcca
     1261 gcctggtatt tgcgccagcc atcccgtcac cgaactgcga gccggtgtgg atctggtcaa
     1321 actccagatt catctgggcc gtgacgggca ccttgaaacc catcctccgg cactctcgcc
     1381 agcccacgcc atgggggttg aactggtcgc cattcctgaa gcaaccgatc ttccagccag
     1441 ttccaagatt gtcgaatcac taaaaaccgt gacggggccg gggcttcgga ttgatccagg
     1501 gatcagtgaa ggcaatctgc tggcccagcc gttcttaccg tcacgatgga tcagtctggt
     1561 caccaccagc gggcataacc gggcggaaac cctgtccaga ttaaaacgcg ccttgctgaa
     1621 ctcagcctgt gtcatccacg gttaccgctg caacaagtcg caacttctgg ccatgctcaa
     1681 tcgcccggat gtcattgatg gccacaccac accgattcct gcggccacca ttgctccgct
     1741 caacccgctc tttggtaaag tggcgttaat tcaggcagcc attgaagtct atctggctga
     1801 tttttcagac gagcacgctc aatttgtcac gtctgcggct cgtggtcggc cacaggtccg
     1861 aggtgaagcg ggtctgacgg tcgaactcgg ctatcagggc gaagtctatg ccctgaccgt
     1921 gtatcggctt ggcgaccaaa actatcgaat tgacgttgat ggccagcaaa ttccagtcac
     1981 cgtggcctgg agcaatcgcc acgaacgccg actgatcgtc gccggtcaga attttcatac
     2041 cttcccggtg gtgacccagc ccaactttac gattgaagtt gaaggcaccg aacaccacat
     2101 ttcccgtgaa gatttcggcg tggtacgctg tccaacgcca gcggtcgtag tatcggtcgc
     2161 cgtgaaagaa ggcgacgaag tcaaaaccgg cgatgtggtg gccgtggtcg aatccatgaa
     2221 actggaaatg ccgatcaatg cgccatatgc cggaaaagtc cgtaaagtgc tggtcctgcc
     2281 gaatgtccag gtggatatcg gcgccccgct gatccagttt gaactttcca gccatccggc
     2341 tgccggatct gccggcgaac ggctgcggtt ttcaaatttg gtgccagccg caccagactc
     2401 actgcaggcc cggtgtcata ccaattttga agcgttccgg agcctgctgc ttggctatga
     2461 cgtcgctcca gccgaagtca aacgactgat ggctgaccgt ggacaactct gtcatcaatt
     2521 gccggtgact gacccggacc tgattggaaa cgaagatgag atgttgacga tctttgttga
     2581 cctctgctcg ctcttccaac gcctggcctt aaccgaagat cacgaagccg ccgaagccct
     2641 cgcgtcccag gaatacctgt tgacctatct gcgaacgttt gacacccagg gaaccgagct
     2701 tccgcaggca ttcctcgata aactgctgcg caccctcgcc cattttggtg tctatagcct
     2761 gcggcgcacc cccgaactcg aagaagcact cttgcggctc tacaaatctc acctgaagat
     2821 ggatcagcac ctgaccctga tgctgagttt gcttgaacga cgactggcca accacgaagc
     2881 catgcaaccg gcgctcagtg cgtcattcca gtggctctta aatcggacaa ttgaagaaac
     2941 cctcggcagt tatcccagcc tgagcgaact ggctcgcgaa gttcgcttcc gcctctatga
     3001 tgaagccatt tttgaacagg cacgcaaacg tatttatgcc gacgtcgaag aacatttaaa
     3061 ctacctggaa gaatctcccg gtgctgagga tcgggaagtt catatccagg ccctggccaa
     3121 ctgtccacaa cccctggccg gattgctggc caaacggttt ggcgatatgt cgctggccat
     3181 gcaacagttg attctggaag tgctcacccg gcgctattac cgcatccgca aacttgagaa
     3241 cttccaaaac ctgctt
//
LOCUS       DAOQDO010000471         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22733_length_3255_cov_3.609375, whole genome shotgun sequence.
ACCESSION   DAOQDO010000471 DAOQDO010000000
VERSION     DAOQDO010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22733_length_3255_cov_3.609375"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKZ53_08775"
     CDS             <1..556
                     /locus_tag="PKZ53_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ40569.1"
                     /translation="HARHVVAQTVERFGRVDILINNAGITWKAPAETMSLDRWNQVMT
                     VNVTGAFLMAQAVYPEMLKVGGGKIVNIASVAGLAGSSAAVMDAVGYNASKGAMISMS
                     RDLAVKWAPKGIYVNAVAPGFFPTRMTEALLSEHQEEVETATPLGRVGQENELKGAVL
                     FLASRASDFVVGQVLVVDGGATAW"
     gene            562..1422
                     /locus_tag="PKZ53_08780"
     CDS             562..1422
                     /locus_tag="PKZ53_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40570.1"
                     /translation="MKRRVAVLHHETHYHRKTPYLIDFLIDIWRASGIEVIEVFGTSD
                     FIPADVAILHIDVSVVPDTYLHFASQYPVVLNNLVKDICKATISLHLVSGDSPYQGPV
                     IVKSNLNSAGYPERLIEHLESKPSLTLLQRLGRWITPRPTRPPVFQSNQDYQIFDRLS
                     DVPRSFFNNSEVVVEKFFPQMEDGLYCVQNYVVLGKNGYGTFLAGEQPIVNVGTCKRF
                     EINEPASEIIQYCTHHQIEYGKIDYVMSDGKAIVLDVNKTVGAGNFSRPAALFKCYEH
                     LASGIYAYFG"
     gene            1457..2323
                     /locus_tag="PKZ53_08785"
     CDS             1457..2323
                     /locus_tag="PKZ53_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40571.1"
                     /translation="MKKRIVILFHEFQYDRSSTYMIDELAEIWREEGLEVIELYGLDR
                     FVPADIVILHIDLSVVPPEYLEFTRQYPVALNTRLTDIRKVTISQNLIKPGDPYGGPV
                     IVKSNLNYAGAPERKLKEAQLRQSPFWLKRLQHRIFPFLKKLPVFQSNLEYQIFDRVE
                     DVPNSLFNHPDVVVEKFFQCRHEEFYCIHNLYIFGNLRTGIRLAGHHPIVNGRTYQRI
                     DFIDPDSEVLEQLADIPLDYGKIDYVFHDGKVVIIDINKTIGDSRKVFQRTPELIRFY
                     KKLAAGIHVYLG"
     gene            2362..>3255
                     /locus_tag="PKZ53_08790"
     CDS             2362..>3255
                     /locus_tag="PKZ53_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40572.1"
                     /translation="MTARIAILFHEKNRGWRVPYTIDLLAEFWKQDGLEVTEIYGVDT
                     FTPADVLIVHVDLSVVPNKYLEFARQYPVALNAGLADVRKSSYSRYLVTAGDGYQGPV
                     IVKSNLNFAGVPERLLSQSGIPRQVVRLRKWLGHHGLLPDVSPFQAPMDYPIFPSLQD
                     VPRKFRDNPQVIIEQFLPQIEDGLYHVFNFHFLGDRLTCARLSSPQPIVNGKTQIHSE
                     SVEPHPQIIELRQKMNFDYGKFDYVVRDGQVIFPDRQLMLLARDVLARCPGATILYDV
                     KCSQRLADEIRAAGGVPLMWKT"
BASE COUNT          796 a          786 c          812 g          861 t
ORIGIN      
        1 gcatgcccga cacgtggttg cccagacggt cgaacgcttt ggccgggtgg atattttgat
       61 caacaacgcc ggaattacct ggaaagcccc ggcagagacg atgtcgcttg accgctggaa
      121 tcaggtcatg acggtcaatg tcaccggcgc gtttttgatg gcccaggcgg tctatccgga
      181 aatgctcaag gtcggcggcg gaaaaatcgt caatatcgct tcggtggcag gtctggctgg
      241 ttcgtctgcc gcagtaatgg acgcagttgg ctacaatgca agcaaaggcg ccatgatcag
      301 tatgtcgcgt gatctggctg tgaagtgggc ccctaaagga atttatgtca atgccgtagc
      361 gccgggcttt ttcccaaccc gaatgactga agccttactt tcagagcatc aggaagaagt
      421 cgaaaccgca acgccgttgg gtcgtgttgg tcaggaaaat gaactcaaag gcgcggtctt
      481 gttcttagcc tcgcgcgctt cggattttgt cgttggccag gtgctggtcg tggatggcgg
      541 tgccaccgcc tggtaaagga tatgaaaagg cgagtcgcgg ttctgcatca cgagacgcat
      601 taccatcgga aaacaccgta tttgattgac tttctgattg atatctggcg agccagtgga
      661 attgaggtga tcgaagtctt tggtacctca gactttatac cagcagatgt tgcgattttg
      721 cacattgatg tgtcggttgt tcctgacaca taccttcact ttgccagtca gtatccagtt
      781 gtgttaaaca acctggtgaa agatatttgc aaggcaacca tcagccttca tcttgtttcg
      841 ggtgattcac cataccaggg accagtgatt gtcaaatcca acctgaattc ggcggggtat
      901 ccggaacggc tgattgaaca ccttgaatca aaaccttcac tgacgttgct ccaacggctt
      961 gggaggtgga taaccccacg tcccaccagg ccaccagtgt ttcaatcaaa tcaggattac
     1021 cagatttttg accggctgag tgatgtaccc cggtcatttt tcaacaattc agaggtcgtg
     1081 gtcgaaaaat tctttcccca aatggaagac gggctgtact gtgtccaaaa ttacgtcgtg
     1141 cttggcaaaa acgggtacgg cacgtttctg gcaggggaac agccgattgt gaatgtagga
     1201 acctgtaagc ggtttgaaat caatgagcca gcttcggaaa tcattcagta ttgtactcat
     1261 caccagattg aatacggcaa aattgattat gtgatgagtg acggaaaagc gattgtgctg
     1321 gacgtcaata aaacggttgg ggctggcaat ttctctcgac cagcagcgct tttcaagtgt
     1381 tacgagcatc ttgcttcggg tatttatgcc tactttggtt gagcgtcgtt gaacaatctg
     1441 aggctggctg gtttttatga aaaaacggat tgtcattctg ttccatgagt ttcagtatga
     1501 tcgctcatca acttacatga ttgatgaact ggctgaaatc tggcgagaag aaggactcga
     1561 agttattgaa ctctatggcc ttgacagatt tgttccggca gatattgtga tccttcatat
     1621 tgatctttca gttgtcccgc cagagtacct cgaatttacc cggcaatatc cagtggcgct
     1681 caataccagg ttgaccgaca ttcgaaaggt gaccatcagt cagaatttaa tcaaacccgg
     1741 tgacccgtat ggtgggccgg ttattgtgaa atccaatttg aactatgccg gagcacctga
     1801 acgaaaactg aaagaagctc aacttcgcca atctcctttt tggctgaagc gactccaaca
     1861 tcggatcttc ccatttctga agaagttacc tgtctttcaa tcaaacctgg agtatcaaat
     1921 ctttgatcga gtagaggatg tcccaaattc tttgttcaac catcctgatg tcgtggttga
     1981 aaagtttttt cagtgccgac acgaagaatt ttattgcatc cataatctgt atatctttgg
     2041 gaatctccgg actggtatcc ggttagcggg ccatcatccg attgtcaacg gcagaaccta
     2101 tcaacgcatt gactttattg accccgattc ggaggttttg gaacaattgg ctgacatccc
     2161 acttgattat ggaaagattg actatgtgtt ccacgacgga aaagtcgtga ttattgacat
     2221 caacaaaacc attggtgaca gccgcaaagt tttccaacgg acacctgaat tgatccgatt
     2281 ctacaaaaaa ctggccgccg gaattcatgt ttaccttgga tagtttcctt tcaatgagcc
     2341 aaaccaggat tcactctttc aatgacggca cgtatcgcta ttctctttca tgaaaaaaat
     2401 cgtggctggc gagttcctta cacgattgac ctgctggccg agttctggaa gcaggatggc
     2461 ctggaagtga ctgaaattta tggggttgat acgtttaccc cggctgatgt cctgattgtt
     2521 cacgttgatt tatcagtggt tccaaacaaa tatctggaat ttgcccgcca gtatccggtt
     2581 gccctcaatg ctgggttagc tgatgttcgc aaatcatcct atagtcggta tctggtcaca
     2641 gccggagacg gctaccaggg cccggtcatc gtcaaatcaa acctgaactt tgccggagtt
     2701 ccagaacgac tcctttcgca aagcggcatt ccgcgacagg tcgtccggct ccgaaaatgg
     2761 cttggccacc atggtttgct gccagatgtc tcgccatttc aggctccaat ggactaccca
     2821 atttttccga gcctgcagga tgttccacga aaattccgtg acaacccaca agtcatcatc
     2881 gagcaatttc tgccccaaat cgaagatggt ctgtaccatg tcttcaactt tcatttttta
     2941 ggtgaccgcc tgacctgcgc tcgattgtct tctccgcaac cgattgtaaa tgggaagacc
     3001 caaattcact ctgaatcagt cgagccgcac ccgcaaatca ttgaattgcg gcagaaaatg
     3061 aactttgact atggaaagtt tgattatgtg gtgcgcgacg gtcaggtgat cttccccgac
     3121 cgccagttga tgctgctggc gcgcgacgtg ctcgcgcgct gtcccggcgc gacgatcctc
     3181 tacgacgtca agtgcagcca gcgcctggcc gacgagatcc gcgccgccgg cggcgtgccg
     3241 ctgatgtgga agacc
//
LOCUS       DAOQDO010000472         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22748_length_3254_cov_3.543920, whole genome shotgun sequence.
ACCESSION   DAOQDO010000472 DAOQDO010000000
VERSION     DAOQDO010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22748_length_3254_cov_3.543920"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /gene="argB"
                     /locus_tag="PKZ53_08795"
     CDS             <1..514
                     /gene="argB"
                     /locus_tag="PKZ53_08795"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNJ40573.1"
                     /translation="DAELITAHKRPPRTVTDLQTGETKLVDFGFVGDLVSVNPTLLRV
                     LLAARMVPVVSPLVADETGQVFNTNADTVATELAIALRADQWIAATTVDGVLDANQQV
                     IPEINPALFEQLAAAKVITGGMYPKLTNAFTALNGGVTSVHIVNGQRPGTLVDVVIGQ
                     PVGTRIQVTQ"
     gene            511..1758
                     /locus_tag="PKZ53_08800"
     CDS             511..1758
                     /locus_tag="PKZ53_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNJ40574.1"
                     /translation="MTHRFLNENPEPGTRNPAFENPEIEAIFPKLVEIRRDLHRHPEL
                     SNREVRTSKIVAEHLTNLGLEVTTGIAHHGVVGILRGKQPGPVVAVRADMDALPIQEV
                     RDVPYKSCVPGVMHACGHDVHTTIGLGTAEILAHRTNDLTGTVKFLFQPAEEGAPVGE
                     DGGAKMMIREGVLEDPSPEVIFGLHVYPPLEAGQIGYVAGPAMAGASRFEIKIKGKKA
                     HGAYPHTGVDAVLVASHTVVALQTISSRMVNSQDPVVVTVGSIHGGNRGNILADEVVL
                     IGTVRALSDDVMDQVEQRMLQILAGITTSFGAEFEVNFARQVPALVNDPSLTSRMVPT
                     FERLVGKANAIKTPPRMGAEDFAFFAAARPSLFFSLGIANLERGITAGIHTPEFDVDE
                     TCLKTGVAAMVELVLEIGRTRHG"
     gene            complement(1840..2391)
                     /locus_tag="PKZ53_08805"
     CDS             complement(1840..2391)
                     /locus_tag="PKZ53_08805"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ40575.1"
                     /translation="MRCGIWCRIQITAWLLMISVAVTTGHASQTTGSKTQTKTQKPLI
                     VLKSKNRPTSPTPDPNDRPEPTSENPKNRTTSPTTTSGGLSDVLIENRRPSSMPPIRT
                     LHSVSPVYPPELRDAGITGTVKVRVDISSEGDVTNVSAVSGPPEFYDAAFEAAYQWKF
                     EPVLLNNRPVRISGVLTFGFVRW"
     gene            complement(2571..3125)
                     /locus_tag="PKZ53_08810"
     CDS             complement(2571..3125)
                     /locus_tag="PKZ53_08810"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ40576.1"
                     /translation="MRREKRYQFLIFMCLLLVIEATQVGLVTATSFGQKQRRRTQSKR
                     KKVAPIQKMTPSPEQNTDVPGDVKKTTDETPAKENSSNTTQTDSSNAENQRYNGVIRG
                     NAIYKVTPDYPREARNAGISGDVVVRILISQTGDVIEAKAEGGPPELHEAAVEAALQW
                     KFKPTLLNNEPVKVTGILTFRFSL"
BASE COUNT          782 a          844 c          835 g          793 t
ORIGIN      
        1 tgatgccgaa ctgattaccg cacacaaacg gcctccgcga acggttaccg acttgcaaac
       61 cggcgaaacc aaactggttg acttcggctt tgtcggagac ctcgtttcgg tcaatccaac
      121 cctgctcaga gtcctgctcg cagcccgaat ggttccggtt gtctccccgc tggtcgccga
      181 tgaaaccggc caggtgttta acaccaatgc cgacacggtg gcgaccgaac tggcgattgc
      241 gctccgggct gaccagtgga ttgccgccac aaccgtggat ggagtgctcg atgcaaacca
      301 gcaggtgatt cccgaaatca acccggcttt atttgaacaa ctggcggctg ccaaagtcat
      361 cactggtgga atgtatccca agctaaccaa cgcgtttacg gccctgaacg gtggcgtcac
      421 atcagtccac attgtgaacg gtcaacgccc tggaacgctg gtggatgtcg tgatcgggca
      481 accggtggga accaggattc aggtgacaca gtgacacata gatttttaaa tgaaaacccg
      541 gaacccggaa cccggaaccc ggcatttgaa aacccggaaa ttgaagctat cttcccaaaa
      601 ctggttgaaa ttcgccgcga cctgcaccga catccagagc tttccaatcg tgaagtccgc
      661 acgtcaaaga ttgttgcaga gcacttaacc aaccttggcc tggaagtcac caccggcatt
      721 gcgcatcacg gtgtggtcgg tattctgcgc gggaaacagc cggggccagt ggtggccgtg
      781 cgggcggata tggatgcgct tccgattcag gaagtccggg atgtgccgta caaatcctgt
      841 gttccgggcg tgatgcacgc ctgtgggcac gatgttcaca caacgattgg tttagggacg
      901 gcggaaattc tggcgcatcg cacaaatgat ctgaccggca ccgtcaaatt tctcttccaa
      961 ccggctgaag aaggcgcccc ggttggcgaa gatggcggcg ccaaaatgat gattcgcgaa
     1021 ggcgtgctgg aggatccttc tcccgaagtg atttttggcc tccacgtcta tccgccgctt
     1081 gaagctggcc aaatcggtta tgtcgccggc ccggccatgg ctggtgccag tcggtttgag
     1141 atcaaaatca aaggcaaaaa agcccacggc gcctatccgc acactggcgt ggatgcggtg
     1201 ctggtggcct cgcacacggt tgtggcgctt caaacgattt ccagccgaat ggtcaattcg
     1261 caggatccag tggtggttac ggtcggaagt atccacggcg gaaatcgagg aaatatcctg
     1321 gcggatgaag tggtgttgat tggaacagtt cgagctttaa gcgatgatgt gatggatcag
     1381 gtcgaacagc gcatgcttca aattttagct gggatcacaa ccagttttgg agctgagttt
     1441 gaggtgaact ttgcgcgtca ggtaccggca ctggtcaatg atccatcatt aaccagccga
     1501 atggtcccaa cctttgagcg cctggttggg aaagccaacg ccatcaaaac gccgccgcga
     1561 atgggcgccg aagattttgc gttttttgct gccgcccgcc cgtcgctctt tttctcgctt
     1621 ggcattgcca atctcgagcg cgggatcacg gctggcattc acacaccaga atttgatgtt
     1681 gatgaaacct gcctgaaaac tggggtcgcc gccatggtgg aactggtttt ggagataggg
     1741 cgaacacgac atggctgaag acatcagatc aagatactca aaaccccatc tcaactcagc
     1801 tccgggagtc actttcttca accccatatc ttctttcact taccatctga caaacccaaa
     1861 tgtcaggacg ccacttatcc ggacagggcg attattcaaa agtaccggtt cgaacttcca
     1921 ttgataggcg gcttcaaacg ccgcatcata aaactcaggg ggaccgctca cggcactgac
     1981 attggtcacg tcgccctcag aggaaatatc aactcgaact ttgacggttc cggttattcc
     2041 agcatcgcgt aactcgggtg gatagacggg tgatacgctg tgcagtgtcc ggattggagg
     2101 catgctactg ggtcgccggt tttcgatcag cacatcactg agcccacctg aggtcgttgt
     2161 aggcgaggta gttcgatttt ttggattttc agacgttggc tctggccgat catttggatc
     2221 aggcgttggg gaggtgggcc gattctttga tttcaggaca atcagaggtt tttgagtctt
     2281 tgtttgcgtt tttgatccag tcgtttgtga ggcgtgacca gttgtgacgg ccacagaaat
     2341 catcagcaac caggctgtta tttgaattcg acaccagatt ccacagcgca taaattttac
     2401 ccctctcttt tcaagaatca tttaactttg gttttccgaa atccaggtct gtatgggact
     2461 cagagtctaa ctggtacatc acaccagcaa tcttcaacca ccatacctgg caacgagcgg
     2521 aaagacaatt cgttttcccc tgcctgaccg accatgagca ttgcgaaaac ctatagtgaa
     2581 aaccggaaag tcaggattcc agttactttt acaggttcat tattcaataa cgtaggctta
     2641 aacttccatt gcaatgcagc ctcaaccgca gcctcatgca attcaggtgg tccaccttcc
     2701 gcttttgctt caatgacatc acccgtctgt gaaatcagga ttctcacaac cacatcgcct
     2761 gaaattccag cgttgcgtgc ttcacgaggg taatcagggg ttactttgta gatagcattc
     2821 ccgcgaataa ctccgttata ccgctggttt tcggcgttgg atgagtcagt ttgagtagtg
     2881 ttggaagagt tttcttttgc gggagtctca tccgttgttt ttttcacgtc accgggtaca
     2941 tcggtgtttt gttcagggct gggggtcatc ttttggatag gcgctacctt tttacgtttt
     3001 gattgagtac gacgacgttg tttttgccca aagctagtgg cagttaccaa tccaacctgc
     3061 gtagcctcaa tcacaagcaa caggcacata aaaattaaaa actgatacct cttttcacga
     3121 cgcatacaac agaactcctg ggttgaaaat tcgattgttc aaaagctaac aagccaaggt
     3181 acttgcaatt caacaggtgc ataacactgc aaaactcaaa ataccttacc gggcgctctc
     3241 cgaaaaaaca acct
//
LOCUS       DAOQDO010000473         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22788_length_3251_cov_3.152065, whole genome shotgun sequence.
ACCESSION   DAOQDO010000473 DAOQDO010000000
VERSION     DAOQDO010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22788_length_3251_cov_3.152065"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..2564)
                     /locus_tag="PKZ53_08815"
     CDS             complement(213..2564)
                     /locus_tag="PKZ53_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNJ40577.1"
                     /translation="MSLDFLKTLNPQQREAVTTTDGPLLVLAGAGSGKTRVITFRMAY
                     LIQERGIRPGHILAVTFTNKAAAEMKERVTQLLPQGQTRSTPMVSTFHSFCVRLLRRD
                     INRLGGNYTGDFTIYDSDDQAKIVKNCLKDLNIDEKMIQPRAVQHAISGAKNQGKTIE
                     DFLQTNDPKKVTIGRVFKLYEERLANGNALDFDDLLLKSVRLLRNFPEVQDYYNDWFK
                     YILIDEYQDTNPPQYSLIRLLTQKQQNLCVVGDPDQSIYRFRAADIRNILDFEKHYPG
                     AKMIKLTENYRSTKVILSAANEVIRNNTERKPKDLFTNNDEGEKIRYFQADSGEAEAA
                     FVVNELNRWQKLNPHIDNRAAILYRTNAQSRLFEEACRRAGLPYVLVGGFSFYQRSEI
                     KDVISYLKLVLNPTDPVAFERIINTPARGIGKATLDILYARAREENSSPWDAMGNLIA
                     EQKLPARTTNALKHFQEFIQRLIDLASQSSLSELVKAVLNESGYLKWLQAEKTEEAES
                     RLLNLEELVNAAVEAELRDETIRDFIDHAALVADTDSLKETAKITLMTIHSAKGLEFP
                     IVFVVGMEEGLFPHQRASMNMHEMEEERRLCYVALTRAKTQLYITHAEWRRQYGEEIF
                     NEPSRFLKEIPTDKITDLTLGQSWLKSISRKTPNALSATASTGLGPKSPAKPPVAPPK
                     SPFPTYNSVDSIQQFFKLQQARKAAEAQNNQPAPKPGIQRPAPPPATPSATARGFNPG
                     DRVRHEKYGVGLILKREDDKLVINFPGFGMKKFVESAVKLERA"
     gene            complement(2760..3065)
                     /locus_tag="PKZ53_08820"
     CDS             complement(2760..3065)
                     /locus_tag="PKZ53_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNJ40578.1"
                     /translation="MLWIIAGGGLGSGLFFCWVLIRGWIRKQHPDLGFTAGDCGITES
                     ALVPFGYVWIAGEIWPAQASTPIPAGVPVRFIGVDGIRLCVECVSPKSQKTSRSQTR"
BASE COUNT          722 a          777 c          886 g          866 t
ORIGIN      
        1 tgtttaacct gtcaccgtag tgcggcggac tgaggcggca tccgccggta cctatcgatt
       61 cttccccatc gtttcccgtg atttgcacgg gggtctggtc aactggagct tgaacccatc
      121 tccccacacc ggccatccgt caggatggcg tcgtggcgac acgactcaag ctcggccctt
      181 cagggccgag tctgttgact cttgatttcc ggtcaggcgc gctccagttt taccgccgat
      241 tcaacaaatt ttttcatgcc aaaaccaggg aagttgatca caagtttgtc gtcttcgcgc
      301 ttgagaatga gcccgacgcc gtatttttcg tgacggacac ggtcacctgg gttaaaccca
      361 cgggcagttg ccgacggtgt ggccggcggc ggcgctggtc gctgtattcc aggtttggga
      421 gctggctggt tgttttgggc ttcggcggct ttgcgggcct gttgcagctt aaaaaactgt
      481 tgaatgctgt caacgctgtt ataggtcgga aacggggatt tgggcggtgc cacaggtggt
      541 ttggctggtg atttgggacc caggccggtt gaagcggtgg cactcaacgc atttggggtt
      601 ttccgcgaga ttgatttcaa ccagctttgg ccgagggtca ggtcagtgat tttgtcggtg
      661 gggatttctt tcagaaaccg ggatggttcg ttaaagattt cttctccgta ctgccgtcgc
      721 cattcagcat gggtgatata caactgggtt ttggctctgg tgagggcgac gtagcacaat
      781 cgccgctctt cttccatttc gtgcatattc attgatgccc gctggtgagg gaacaatcct
      841 tcttccatcc caaccacaaa cacaatcggg aactctagcc ctttggcgct gtggatcgtc
      901 atgagcgtga ttttcgcggt ttctttgagt gaatcggtgt cagccaccag cgccgcatgg
      961 tcaatgaaat ctcggatggt ctcgtcgcgg agttcagctt cgaccgctgc gttgaccagt
     1021 tcttcaaggt tgagcagccg gctttcggct tcttcagttt tttcagcctg gagccatttg
     1081 agatatccgc tttcgttgag cacggctttg accagttctg aaagtgaaga ctggctggca
     1141 agatcaatga gccgctggat gaattcctgg aaatgtttga gcgcattggt ggttcgggct
     1201 ggcagctttt gttcggcaat cagattgccc attgcgtccc agggcgagct gttttcctcg
     1261 cgtgctctgg catacaaaat atcaagcgtc gctttcccga ttccgcgagc cggagtattg
     1321 ataattcgtt cgaaggcaac tggatccgtc gggttgagca ccagtttcag ataggagatg
     1381 acatccttga tttcagatcg ctggtagaag gaaaacccac caaccagcac atagggcagg
     1441 cccgcccggc gacaggcttc ttcaaagagt cgtgattggg cgttggtgcg atataaaatt
     1501 gcggcgcggt tgtcgatgtg tggattgagt ttttgccagc ggttgagttc gttgacgaca
     1561 aaggcggctt cggcttcacc tgagtcagcc tgaaagtacc ggattttttc gccctcgtcg
     1621 ttgttggtga acaagtcttt cggtttgcgc tcggtattgt tgcggatgac ttcgttggca
     1681 gcgctcaaaa tgactttcgt ggagcgataa ttttcggtca gtttgatcat tttggcgcca
     1741 ggataatgtt tttcgaaatc caggatgttg cggatgtccg ctgcccgaaa acgataaatg
     1801 gactgatccg ggtcgcccac gacacacaag ttttgttgtt tttgcgtgag caggcggatc
     1861 aggctgtatt gaggcggatt ggtatcctgg tattcatcaa tcagaatgta tttgaaccag
     1921 tcgttgtaat aatcctgaac ttcgggaaaa tttctgagca ggcggactga tttcaacagc
     1981 aagtcatcga aatcgagtgc gttgccattg gcgagacgct cttcatagag tttaaacact
     2041 cgaccaatgg taactttttt gggatcattg gtttgcagaa aatcttcaat cgttttgccc
     2101 tgatttttgg caccgctgat ggcgtgttga actgcccgtg gctgaatcat cttttcatca
     2161 atattgagat ctttgaggca gtttttgaca attttggcct ggtcgtccga gtcataaatt
     2221 gtgaaatctc cggtatagtt tcctcccaac cggttgatgt cacgccgcag taatcgcaca
     2281 cagaagctat gaaatgtcga aaccattggt gtggaacgcg tctgaccctg tggcaagagc
     2341 tgggtgaccc gttctttcat ttccgctgcc gctttgttgg taaaggtgac cgccaggata
     2401 tgacctggtc ggatgccacg ttcctggatc agataggcca tgcgaaatgt aatcacacgg
     2461 gtttttccag aaccggcacc cgccagaacc agcaatggtc catcggttgt ggtaacggct
     2521 tcgcgttgct gtggattgag ggttttgaga aagtccaggc tcataaaatt tgaatgaaga
     2581 atgaggaatt gagaatgaag aatgaagaat gaagaatgac ctgaatttcg cccaaaagag
     2641 caccacctgg ctcaaagatc agtgccgacc agttcgggaa ctgactcgca ccagggaagt
     2701 ccgctgagct ttgaggggga atgaagcatg aaggtaccaa atttgctcca aactcaatct
     2761 tatcgcgtct ggcttcggct ggttttctga gatttgggag aaacacactc aacacacagt
     2821 cgaatgccat caactccgat gaaccgtacc ggaacaccgg ctggaattgg ggttgaagcc
     2881 tgagctggcc agatttctcc agcaatccag acataaccaa atggaactaa tgcgctttcg
     2941 gtgattccac aatcgccggc agtgaaccca aggtctgggt gctgttttcg aatccagcca
     3001 cggatcagca cccaacaaaa aaacagccca cttccgagtc caccacccgc tatgatccaa
     3061 agcaaaaagt gagaaagcat ctgaggagtg cccgttagaa ccggaggaaa atttcctcat
     3121 cgagtttggc cagtcggcat ttggtttctt ttaactcaat ttcctgcaca aacttaccat
     3181 tttcatagag caccgtgcga cccggtacga gaacgccttg aacgtttcgg aactcataga
     3241 aggattcgcg g
//
LOCUS       DAOQDO010000474         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22868_length_3243_cov_2.685383, whole genome shotgun sequence.
ACCESSION   DAOQDO010000474 DAOQDO010000000
VERSION     DAOQDO010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22868_length_3243_cov_2.685383"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKZ53_08825"
     CDS             complement(<1..578)
                     /locus_tag="PKZ53_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40579.1"
                     /translation="MIILSEATLQNPTTPSEQNNRDLTDAAQMAGCKVYFIPSDFSVC
                     ETAENALWHIPEQPTETAGVWVGFIPSAERYAAIYTEALKKGIHLINSIDEHLTAQEF
                     DRAYPLLSGLTPESRTLTNLEETAPVGEALGFPLFVRGSARSSKSDGWNACVATSPDE
                     FHQIVERYFASQYRSRGRVIARKLVQLKAVRSS"
     gene            1025..1225
                     /locus_tag="PKZ53_08830"
     CDS             1025..1225
                     /locus_tag="PKZ53_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40580.1"
                     /translation="MASLYSQRRLKDLEKLLDEQYENLVFPELERDRIADMAQEFYLN
                     KKRENSKSKAASSRRTPKCFYG"
     gene            1307..2644
                     /locus_tag="PKZ53_08835"
     CDS             1307..2644
                     /locus_tag="PKZ53_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ40581.1"
                     /translation="MKRLVVTVLLMFVLSSIALGQKQKIFPYPIHKKQLPNGLKLVVV
                     PFDSPGIMAYYTVVRVGSRNEVEPGVTGFAHFFEHMMFRGTPKYSNEKYQAILKDLGV
                     DSNAFTSDDWTCYHSTAPASALETIMDMESDRFQNLKYSLEDFQKEAKAVLGEYNKSA
                     ASPFLAINEAQRDTMYSKHTYKHSTIGFLKDIVDMPNQYDYSLKFFDRFYRPENCTVM
                     VVGDVKPEPFFKLAEKYYGPWKHGNHVADIPVEPAQTGEKVADIPWKGRTLPYLVVAF
                     HAPVFSTETKDKVALNLLAKLAFGNTSALYQKLVLSEQRVDLLAASADDSRDPGSFDI
                     IVRVKNEAELAKVRDEVYAKLEELKVKPVADDELAEVKSNFRYNFAMQLRTPDEVAYT
                     LGNYINLAGEPEAVNKVFELYDQVTPADIQRVAQQIFTKENRTVLTLKGGSAK"
     gene            2641..>3243
                     /locus_tag="PKZ53_08840"
     CDS             2641..>3243
                     /locus_tag="PKZ53_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNJ40582.1"
                     /translation="MKFTTLFNRLMLGLVVTATMAPGLVQAIGPKSDTSKAKSGISKV
                     VLPSQSPLITIRVMFRAGSAYDPPGKEGIAKLTASLIEEGGSQSLTYKDILKKLYPMA
                     ADVSVKVEKETVTMVGEVHKDHLDKFYAIFSDVLLHPRLDQAEFDRVREDAVNYLKTS
                     LRSTNDEEFGKQTLETEIFRGHAYDHVIGGSVRSLNALKLE"
BASE COUNT          823 a          835 c          819 g          766 t
ORIGIN      
        1 gaactccgaa ctgctttcaa ttgaaccagt ttacgggcga tgacccgtcc acgcgaacga
       61 tactgggaag caaaataccg ctcgacaatc tggtgaaatt cgtcagggga agtcgcaaca
      121 caggcgttcc agccatcgga tttgcttgat cgggctgaac ctcggacaaa aagcgggaag
      181 ccgagcgctt cgccaaccgg ggcggtttct tcaaggttgg tcagtgtcct gctttcgggt
      241 gtgagtcctg agaggagcgg gtaggcccgg tcaaattcct gggcggtcag gtgttcgtca
      301 attgaattga tcaggtggat gccttttttg agcgcttcgg tgtaaatcgc cgcgtagcgt
      361 tccgctgaag gaataaatcc aacccacacg cctgccgttt cggttggctg ttccggaata
      421 tgccagagtg cgttttcagc cgtttcacag actgaaaaat cactcggaat gaaatacacc
      481 ttgcacccgg ccatctgtgc cgcatcggtc aaatcgcgat tgttttgttc gctgggcgtg
      541 gttgggtttt ggagcgtggc ttcactgaga ataatcatag ggtttagggt tcggggttca
      601 gggttcaggg ttcttttcac acataggttt ttggttctca tcctgaaagg atggagcaaa
      661 gttagccggt ggtcagcgtt gctttgaacg cgccaccacc ggatgcgatt catctcccga
      721 tttctccgcc cggtccgcgc cccattgggg cgcggaccgg gcggaggagg gagagtaacc
      781 tctgtttccg gtggtagctc ccaaaacctc gcaaccgacc ggctaaactt gctccatcct
      841 ttcaggatga gaaccacgcc cacgcggtag gcgtcggctc agtcccgttt atttcgcttt
      901 ctcccccggc ctgacagatt tgtccgcttt gcgaatgtga gagcttttca tccaggcgat
      961 gattggcgta ttgttgctgt aacctcattt atatctttta ttttcactcc tggataattt
     1021 tcttatggca tcgctctaca gccagcgacg attaaaagac ctggaaaaac ttttggatga
     1081 gcaatatgaa aacctggtat ttcccgagct tgaacgtgat cgaatagccg acatggctca
     1141 agaattttac ctcaataaaa aaagggaaaa ttcaaagagc aaagcggcgt caagccgccg
     1201 cactccaaag tgtttttacg gctagacccg ctgccacttg tccgccataa tccctggcaa
     1261 tccaaacatt caccttcatt cctacaagga gagtaaacgc cacttgatga aacgccttgt
     1321 cgttacggtt ttgctcatgt tcgtgctcag ttcaatcgct cttggacaaa agcaaaaaat
     1381 cttcccttat ccaattcata aaaaacaact tcccaacggt cttaaactgg tggtcgtgcc
     1441 gtttgacagc cctggcatta tggcctatta caccgtagtc cgggttggtt cgcgcaacga
     1501 agtcgagcca ggcgtcaccg gatttgccca cttctttgaa cacatgatgt ttcggggcac
     1561 gccgaaatat tcaaatgaga agtaccaggc catcttgaaa gacctggggg ttgattccaa
     1621 cgccttcacg tcagacgact ggacctgcta tcacagtacg gctccggcca gcgcgcttga
     1681 aaccattatg gatatggaat cagaccgctt ccagaatctg aaatattcac tcgaagattt
     1741 tcagaaagaa gccaaggccg tgctcggaga atacaacaaa agtgccgcgt ctccgtttct
     1801 ggccatcaat gaagcccaac gcgacacgat gtatagcaaa cacacctaca aacattcgac
     1861 gattgggttt ttaaaagaca ttgtggacat gcccaatcaa tatgactaca gcctcaagtt
     1921 ctttgaccgg ttttatcgcc ctgaaaactg caccgtgatg gtggttggcg atgtgaagcc
     1981 ggaaccgttc tttaaactgg ctgaaaaata ctacggtccg tggaaacacg gcaaccatgt
     2041 ggctgatatc ccggtcgaac cagcccaaac cggagaaaaa gtcgctgata ttccatggaa
     2101 aggccgcacc ttaccctatc tggtggtggc atttcacgcg ccagtatttt caactgaaac
     2161 caaagacaaa gtggcgctca atctgctggc aaaactggcc tttggcaata catcagcgct
     2221 ttatcaaaag ctggtgcttt cggagcaacg agttgatttg ctcgctgcca gtgcggacga
     2281 cagccgtgat ccaggttcgt ttgacatcat cgttcgggtg aaaaatgaag ccgaactggc
     2341 caaagtccgc gacgaagtct atgccaaact ggaagaatta aaggtcaagc cggtggctga
     2401 cgatgaactg gccgaggtga aatcaaactt ccgctacaac tttgccatgc aactgcgaac
     2461 cccagacgaa gttgcctata cgcttggcaa ctacatcaat ctggcgggtg aacccgaagc
     2521 cgtcaataaa gtcttcgaac tctatgacca ggtcactccg gctgatattc agcgcgtggc
     2581 ccaacagatt ttcaccaaag aaaaccgcac cgttttgaca ttgaaaggag ggagcgcgaa
     2641 atgaagttca ccactctttt caatcggttg atgctggggc tggtggttac agcaacgatg
     2701 gcaccaggac tggtgcaggc aatcgggcca aaatccgata catcaaaagc caaaagcggc
     2761 atcagcaaag tggtgctgcc cagtcagtca ccattgatta ccatccgggt gatgttccgg
     2821 gctggttcgg cctatgatcc gccaggaaaa gaaggcatcg ccaaactgac cgccagtctg
     2881 atcgaagaag gcggcagcca gtcgttgacc tataaagaca tcctcaagaa gctctacccc
     2941 atggccgccg atgtctcggt caaagtcgaa aaagaaaccg tgacgatggt cggggaagtc
     3001 cacaaagacc atctcgacaa gttttatgcg attttctcgg atgtgttgct ccatccccgg
     3061 cttgatcagg ctgaatttga tcgggttcgc gaagatgcgg tcaattacct gaaaacctcg
     3121 ctccgcagca ccaatgacga agaatttggc aaacaaacgc ttgagaccga aattttccgg
     3181 ggccacgcct atgatcacgt gattggcggc tcggtgcgtt cgctcaacgc gttgaaactg
     3241 gag
//
LOCUS       DAOQDO010000475         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22920_length_3237_cov_3.786926, whole genome shotgun sequence.
ACCESSION   DAOQDO010000475 DAOQDO010000000
VERSION     DAOQDO010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22920_length_3237_cov_3.786926"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKZ53_08845"
     CDS             <1..631
                     /locus_tag="PKZ53_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40583.1"
                     /translation="GPMFSEEGFHLGTGRKFLKEIAVNATRGEGEYSLNDIQQVINQW
                     YPRGLEMFGNEHGGETSVTFGFKDRTNGQAQSEYIREVKGIIDNTNVAILQVLSPGIA
                     ADEAIKLADEATSTGSTVRGIKPEQLVFLPARNFFRKRGPEEVIFKPVDLQGATITQD
                     GKPLDTTAYLNYLRTVLPTGFTQSREFGKYADQLHDAHSTVVNYGYGLN"
     gene            931..1170
                     /locus_tag="PKZ53_08850"
     CDS             931..1170
                     /locus_tag="PKZ53_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNJ40584.1"
                     /translation="MSIETVEALKAELLSSHSEYRELAREHKRCETRISELLALPFPS
                     QDEQTEAAFLKKKKLGLKDRMEEIVQTYSKSRTNH"
     gene            1482..2102
                     /locus_tag="PKZ53_08855"
     CDS             1482..2102
                     /locus_tag="PKZ53_08855"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNJ40585.1"
                     /translation="MAKEGYPFILFFLIPTIVLAVLQWWILAVACLVLTLFMMHFFRD
                     PERTIPTDTNTVVAPADGKITRVIKVEPDNPNSPTLISIFMSPLNVHVNRAPIPGVIR
                     EVKHTKGRFVNAMSDEASLVNEQNTITLENDRMTVVMKQIAGFIARRCVFFKQPGDRV
                     GLGERVGLIKFSSRTDLILPAEVQVIVHPGERVYGGTTIIGRIDQK"
     gene            2099..3040
                     /locus_tag="PKZ53_08860"
     CDS             2099..3040
                     /locus_tag="PKZ53_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HNJ40586.1"
                     /translation="MSNDEDRNERVIRARQQMRKGVYILPSLITITNIYAGFYSVVEA
                     LKGYKYMGLHNIDMAAHHFNIAAMCIGWAVLCDFLDGRVARMMNATSDFGVELDSLAD
                     VLSFGIAPAVLAYTWGFGGALPGFEKLAWGVSFMYLICGALRLARFNVQAHKPAPPPD
                     PTGAPSKKYFVGLPIPAAAGLIAAIIHFAPWPIPYKLTNTSVMNVNLSLSGDIYAVFL
                     LTLVATLSLLMVSTFKFQSLKSNNPSPGRSPIFTIVGIAMLIFLISSYSEWTLLIIAT
                     LYAVQGPLGKVLSLFRRKSGATVAEDPLLKLKPNQPN"
     gene            3043..>3237
                     /locus_tag="PKZ53_08865"
     CDS             3043..>3237
                     /locus_tag="PKZ53_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40587.1"
                     /translation="MTLDYQSFLSAEALSMKPNAIRALAHLVNRPGITSFAGGVPSPE
                     TFPFEELGDITAHLIRDRGTT"
BASE COUNT          791 a          865 c          780 g          801 t
ORIGIN      
        1 gggaccgatg tttagcgagg aagggttcca tctgggcact ggtcgcaaat tcctgaaaga
       61 aatcgcggtc aacgccaccc gaggcgaagg cgaatacagc ctcaacgaca ttcagcaagt
      121 catcaatcag tggtatccgc gtggccttga aatgtttggc aacgaacacg gcggcgaaac
      181 ctcggtcacc tttgggttca aagatcgcac caacggtcag gcccagagtg aatacatccg
      241 cgaagtcaaa ggaatcattg acaataccaa cgtggcgatt ctgcaggtgc tctcacccgg
      301 cattgcggct gacgaagcta ttaaactggc cgatgaagcc acctccaccg gctcaaccgt
      361 tcgagggatt aagccggaac aactggtgtt tttgccagcc cggaatttct tccgcaaacg
      421 tggtccggaa gaagtcattt tcaagccagt tgacttacaa ggagcaacca ttacccagga
      481 tggaaaacca ctggacacaa ctgcctattt gaattacctg cgaacggtgc ttccgactgg
      541 ctttacccaa agccgtgagt ttggaaagta tgccgaccag cttcacgatg cgcattccac
      601 cgtggtgaat tatgggtatg ggttgaattg agtagggagc aaagcagcaa acatagcgag
      661 gtagtgaggt agtgagcagc aagtagtcag aaaaaatatt ttgtcttacg ttttaaacgg
      721 caagtttcgt tgatgtagct tgacgacttc actatctcac cactcactct ttttggtttg
      781 actattcgct acttcgctac gtcgctccag attcgcgaca gttgctttct taacatttgt
      841 gtaatacagt ggaaacactc caacaggata acagttcctc ttctgttgga gttcaccaaa
      901 tctttctaaa tgcaaggagg aaaaaccttt atgagcatcg aaactgtcga agcgctcaag
      961 gctgaattac tctcaagcca ctcggaatac cgcgagctgg cacgtgaaca caaacgttgt
     1021 gaaacccgca tcagtgaact cctcgccctc cccttcccca gccaggatga acaaaccgaa
     1081 gccgcttttc tcaagaaaaa aaagcttgga ttgaaagacc gcatggaaga gattgtgcaa
     1141 acctatagta agtcccgcac aaatcactaa acctcctctt gcaagttata tattcaattg
     1201 gttttcaacg ccatttcaga aataagtgtg gagtctcttc accaaatctg actggtttca
     1261 gttctcggtt gttgtaaagg agagcgtctc tcatcggcgc tctctttttc tatgggcggt
     1321 tccctgatat agtgtcggcc tgaagtcgcc cagtcagcga cattctcccc atcctatctt
     1381 caactcaatc aaggaagttc gatttattct ggcacccaca ttgtcagatt tgttttgcct
     1441 tcgggctggc agggctgaca aacgtacggg ccatctgatt tatggctaag gaaggctacc
     1501 catttatttt gttttttttg atccccacca ttgtgctcgc agttttgcag tggtggattc
     1561 tggcagtggc atgtctggtt ttgacgctgt tcatgatgca cttttttcgt gatccggaac
     1621 gcaccattcc aactgacaca aataccgtag tggcaccggc ggatggaaaa atcacgcgag
     1681 tcatcaaggt cgagccagat aatcccaatt cacccaccct gatcagcatt ttcatgtcac
     1741 cgctcaatgt ccacgtcaac cgggcaccaa ttccgggggt gattcgcgag gtcaaacaca
     1801 ccaaagggcg ctttgtgaac gcgatgagcg acgaagcttc cctggtcaac gagcaaaaca
     1861 ccatcacgct cgaaaatgac cgaatgacgg tggtcatgaa acaaattgca gggtttattg
     1921 cccggcgatg cgtgtttttc aaacaacctg gcgaccgggt tggcctgggc gagcgcgtag
     1981 gtctcatcaa gttcagttca cgaactgacc taatcctccc ggctgaagtc caggtcatcg
     2041 tccaccctgg cgagcgtgtc tatggcggca caaccatcat tggaaggatt gatcaaaaat
     2101 gagcaatgac gaagaccgca atgaacgtgt gattcgggca cgtcagcaga tgcgcaaagg
     2161 cgtctatatc ctgccgagct tgattacgat caccaacatt tatgccgggt tctattcggt
     2221 ggttgaagcc ttgaaaggct acaaatacat gggcctgcat aatattgata tggcagccca
     2281 tcactttaat attgcggcca tgtgcattgg ctgggcggtg ctgtgtgatt ttctggacgg
     2341 acgtgtcgcc cgcatgatga acgccaccag cgactttggg gtcgaacttg atagcctggc
     2401 tgacgtgttg agctttggga ttgccccagc ggtgctggcg tatacctggg gatttggtgg
     2461 cgcccttccc ggctttgaaa aactggcctg gggcgtctcc tttatgtatt taatttgtgg
     2521 agcgctccgc ttagcccggt ttaatgtcca ggcccacaaa ccggcgccgc cgcctgatcc
     2581 aaccggtgct ccctcgaaaa agtacttcgt agggcttccg attccagccg ccgcgggact
     2641 gattgccgcc attattcact ttgccccctg gccgattccg tacaagctga ccaacacgtc
     2701 ggttatgaac gtcaacctgt ccctctcagg tgacatttat gcggtgttcc tgctcaccct
     2761 ggtggcaacc ctctccctgc tgatggtcag cacctttaag ttccaaagcc tgaagagcaa
     2821 caatccgagc cctggccgga gcccaatctt tacaattgtc ggcattgcca tgctcatctt
     2881 cttaatttct tcttattccg aatggacact cctgatcatt gcaacgctgt atgccgtgca
     2941 gggtccgctt ggaaaagttt tgagtctatt tcgccgtaaa tccggcgcaa ccgtggctga
     3001 agatccactt ttaaaactga aacccaacca accgaattaa caatgactct ggattaccaa
     3061 tctttcttgt cggctgaagc cctcagcatg aaacccaatg ccatccgggc gctggctcac
     3121 cttgtcaacc gtccgggaat caccagtttc gccgggggcg tcccaagccc cgaaacattt
     3181 ccctttgaag aactcggcga cattacggcc cacctgatcc gcgaccgcgg cacaacc
//
LOCUS       DAOQDO010000476         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22941_length_3236_cov_2.452688, whole genome shotgun sequence.
ACCESSION   DAOQDO010000476 DAOQDO010000000
VERSION     DAOQDO010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22941_length_3236_cov_2.452688"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..708)
                     /locus_tag="PKZ53_08870"
     CDS             complement(169..708)
                     /locus_tag="PKZ53_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40588.1"
                     /translation="MRWLDTAFWYESGVKWVRTPKVDMYRILGGVYDIYRGSDRIRLI
                     VLSEIQDAGQNLIWELFSTRPEQIQVAAQEFQARYGEASTILNQLFRYYHMEGLTMPY
                     TMEDFKRDAKEEFLKSLTPEERLKGLPSDELLKHLAPDERLKDLPPEALLKRLTVEDI
                     QTYLKKLLDQSESGSSEKP"
     gene            complement(715..1518)
                     /locus_tag="PKZ53_08875"
     CDS             complement(715..1518)
                     /locus_tag="PKZ53_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40589.1"
                     /translation="MRFPTFCCLCGLIFLLLSGPVFVVSGQRAVQKNKPDPFKPLHNV
                     ACCVTVEPDTSLCAINTNEGAYWFLTGGSYLNSKAITASEVRPSSSVDALDVSPDGKY
                     LAVTHHGEGHLWLYVLDLPQLLADESKYVVVQEINPYPGGVYVTSLDQSFLYFESDWA
                     MHRRVQGETALMAPSELALLKLEKFRMDLKTGQVTPIDPKLNDLGSYYGAALVNPQTG
                     TSDRRYTVLPALAFLKEKSSLPFLKQALKQKSNEPLRGEIEALIEALSK"
     gene            1816..2241
                     /locus_tag="PKZ53_08880"
     CDS             1816..2241
                     /locus_tag="PKZ53_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNJ40590.1"
                     /translation="MPHVGGVVVTHGQLANELLSAAEMVVGEINHIQAVSIGWHDDVE
                     QARSTIAKAVDDTNRGAGVVVLTDMFGGTPTNLVMTYSGSTPIEIITGVNLPMVIKLA
                     AQDGDESLVDIARKVRDQGQKNIYLASELMGKKTAEKEK"
     gene            2514..2801
                     /locus_tag="PKZ53_08885"
     CDS             2514..2801
                     /locus_tag="PKZ53_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNJ40591.1"
                     /translation="MSKELRVLIVNRLGLHARAAARLVNLANQFKSAITVTRTDTGNS
                     ADGKSILSVIFLAAPMGTELSISASGEDEAAAVHALAKLVWDKFGEETDVY"
     gene            2791..>3236
                     /locus_tag="PKZ53_08890"
     CDS             2791..>3236
                     /locus_tag="PKZ53_08890"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNJ40592.1"
                     /translation="MSTSRPEQVEQRVQGLGVSVGIAIGTAVRMDAHGRQALFVRILP
                     NRIPHEIKRLQRAVQVARHHLERIKQRMQREFGVSHGYILDPHILMLEDPLLVKSIEE
                     QINENQVNAEWGVKLAVSKWLSAYESIQDEYLRERTSDIEDVANRL"
BASE COUNT          829 a          772 c          850 g          785 t
ORIGIN      
        1 gccggtcagg aagttcacag agtgcttgcc ggattcgata aaggtgctac gaaacatcgt
       61 caagcacact tttgaaagaa ttcaggagtc tgagttcagg aaaatccagt tatttcaagc
      121 aattagctgt tctcttgaaa aagaaatggt ctcaaacgag cctgtctttc agggcttttc
      181 ggatgaacct gactcagatt ggtcgagcag tttcttcaaa taggtttgaa tgtcctcgac
      241 ggtcagtcgt ttcaaaagcg cttccggggg taaatccttg agccgctcat ctggggccag
      301 gtgttttaag agttcatctg agggtaagcc tttgagacgt tcttcaggcg tcaggctctt
      361 gagaaactct tctttggcat cgcgtttgaa atcttccatc gtgtacggca tggtcaatcc
      421 ctccatgtgg taatacctga acaactggtt cagaatcgtg ctcgcttccc cgtatctggc
      481 ttgaaactcc tgggcggcaa cctggatctg ctctggcctg gtgctgaaca actcccagat
      541 gagattttgc ccggcgtcct gaatttcgct caacacaatc agccgaatcc gatccgatcc
      601 ccgataaatg tcatacacac cacccagaat ccgatacata tcaacttttg gagtgcggac
      661 ccacttgacg ccgctttcat accaaaaagc ggtgtcaagc caccgcactc cacactattt
      721 tgaaagggct tcgatcaagg cttcaatctc cccccggagt ggctcgtttg atttttgctt
      781 cagcgcctgt ttcaaaaagg gaaggctgga tttttctttc aggaaggcca gcgccggcaa
      841 caccgtgtac cttctgtcag atgttcccgt ttgggggttg accagcgccg ctccgtaata
      901 gctgcccaga tcattgagtt ttgggtcaat cggtgtcacc tgaccggttt ttaaatccat
      961 ccggaacttt tccagcttca acaaggcgag ttctgacggt gccatcagtg ccgtttctcc
     1021 ctgaacccgc cgatgcatgg cccaatcgct ttcaaaatag aggaatgact gatcgagcga
     1081 cgtcacatag actccgccgg gatacgggtt gatttcctgc accacgacat atttggactc
     1141 atctgccagg agttgtggca aatcgagcac atagagccag aggtggcctt cgccgtgatg
     1201 ggtgaccgcc aggtatttcc catcgggcga aacatccagc gcgtcaactg aagacgatgg
     1261 gcgcacttcg gacgccgtga tggcttttga gttgaggtat gaacccccgg tcaaaaacca
     1321 gtacgcaccc tcattggtgt tgatcgcaca cagtgatgta tccggttcaa ccgtgacaca
     1381 acacgctaca ttgtgcagcg gtttaaacgg atccggcttg tttttttgaa cggcacgctg
     1441 cccggaaacc acaaataccg gcccggaaag caacaggaaa atcagtccgc aaagacagca
     1501 aaaggtgggg aagcgcatag cctgaaatgg tcctttatga agttgagtct ggtcggtttg
     1561 aagcggaaag aagaggtgtg gcagtgagat caaaaaagag ctgaaagcgt tggttgttct
     1621 atgtttatga cgaattttac attttagaaa cgaaaaattc agccagaatt tgggttggtc
     1681 cagttgagct gaaaaaatca tgccgaaaac ctgtgatgct ccgaaagggc ggctagcatt
     1741 ctgcggcgaa gttatgatat tggttgcagt ttgattacat acgattcaca tattagaaaa
     1801 gggagctatg ccactatgcc acatgttgga ggtgttgttg tgacgcacgg tcagttggcc
     1861 aatgaattgt tgagtgcggc tgaaatggtg gttggtgaaa ttaaccatat tcaggcggtt
     1921 tcaattggct ggcatgacga tgtggaacag gcccgaagca cgattgcgaa ggctgttgac
     1981 gacaccaatc gtggggctgg cgttgtagtt ttaaccgata tgtttggcgg aacgccaacc
     2041 aatctggtga tgacctacag cgggtcaacc cccatcgaaa tcataaccgg cgtgaatttg
     2101 ccgatggtta tcaagctggc ggcgcaagac ggggacgaat ccctggttga cattgcccgc
     2161 aaagtgcgtg accaggggca aaaaaacatc tacctggcca gtgaactgat gggaaaaaag
     2221 acggctgaaa aagaaaaata gtcacccttc acccttttgt cgctttcaag tgatggcggc
     2281 gtccagatga ctttctttga aattttgaac caatacccga atggaatgaa gaatgaagaa
     2341 tgaagaatga agaaataaag ccatttgagg caatatgaaa tggcacacac tctcataatt
     2401 cataatttat tcataattca taattcccgt cggtggttgt ctcaagggga accagtcgga
     2461 tttgccggag cgaaaacttt ttgtagcttt ggtgctggct gggtaaagaa accgtgtcaa
     2521 aagaacttcg agtgctcatt gtcaatcgac ttgggttgca cgcccgcgcg gctgcccgac
     2581 ttgtcaacct cgccaaccag ttcaagagtg caattaccgt gacccggacc gataccggga
     2641 atagtgctga cggtaaaagt atcctgagcg tcattttctt agcggctccg atggggactg
     2701 agttgtcaat atcggcatct ggcgaagatg aagccgccgc tgtgcatgct ctggccaaac
     2761 tggtgtggga taagtttgga gaagaaaccg atgtctacta gccgcccaga gcaagtcgaa
     2821 cagcgtgtcc agggactggg cgtttcggtt ggcattgcga ttgggacggc agtgcggatg
     2881 gatgctcatg gacggcaggc gctctttgtc cgaatcctcc caaaccgaat cccccacgaa
     2941 atcaaacggt tgcagcgggc tgtccaggtg gcacgtcatc acctggaacg gatcaaacaa
     3001 cgcatgcagc gtgagtttgg tgtcagtcac ggctacattc tggatccgca tattctcatg
     3061 cttgaagacc cactcctggt caagagcatc gaagaacaaa tcaacgaaaa tcaggtcaat
     3121 gccgaatggg gcgtcaaact ggctgtctcg aagtggcttt ccgcctatga gtcaatccag
     3181 gatgaatacc tgcgcgaacg caccagcgac atcgaagacg ttgccaatcg gctgat
//
LOCUS       DAOQDO010000477         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22953_length_3235_cov_3.360063, whole genome shotgun sequence.
ACCESSION   DAOQDO010000477 DAOQDO010000000
VERSION     DAOQDO010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22953_length_3235_cov_3.360063"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..770
                     /locus_tag="PKZ53_08895"
     CDS             393..770
                     /locus_tag="PKZ53_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40593.1"
                     /translation="MRLTKFLMTGMILMILIVGGFSPVLATAAFQTSGPNLAVVESMK
                     TKLSLTPDQATKIKDILTKSYEQSEKDRASAAGNKEAFKKLNKTRKKQTKEQIKAVLT
                     QDQIAKFDQVKSDLKSALKPKKA"
     gene            1332..2318
                     /locus_tag="PKZ53_08900"
     CDS             1332..2318
                     /locus_tag="PKZ53_08900"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ40594.1"
                     /translation="MSELQSPSLRELEHFLRISVEANYWLTFADVNPVAVVEEIQEQL
                     MDRRFEWLDGAIYVPNLLTVFVIEPTPEKLEELEVLFNSMVFTKYLYEHITESGFKLF
                     DFMRIEIKVAPTDQQQFAGPCYLFFDWPNANESTEQVTVKYDLQERKILELCEPKPDI
                     PRLARLTALNAEVYRNQYTITRSSVSIGRLRNVIDKHTGQVLRRNDFVFARQQVPDSP
                     NASVSRQHGRISFEDGEFFFYDTGSVNGSAIERAGTTIPVATSTNFLQRVQLHDHDII
                     TLGAARILFEVIHTPEEIGRLQTSGETTQERADSLYDLEGLETLRVFPKDLR"
     gene            complement(2382..>3235)
                     /locus_tag="PKZ53_08905"
     CDS             complement(2382..>3235)
                     /locus_tag="PKZ53_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40595.1"
                     /translation="GVFDFDKPASGDGGPARGAALVSPARTVADAQGNVYILDNEDGA
                     IRRVDAATQTISTFAGNPNGSLSDGQKATDSRLVYPMGAAFGPDGNLYFAEAGTNRVR
                     RVVMATGIIETVVGRSAVQGFSGDGGPAITAQLSAPFDVAFDRTGRLYIADAGNHRVR
                     VVDPSGRISTLAGNGETGFNGDNRPASIASLTFPISLAIDGANNVLIADYQDARVRLV
                     NTATQLITTVAGNGQYGSAGDGGPATTAQLFGPADIAVDSLGNLVISDQINNRIRVVR
                     QAVQPVR"
BASE COUNT          837 a          856 c          772 g          770 t
ORIGIN      
        1 cagtcgtttt cctggtcaat ccccaccaat ctggcaaaaa ctaagacagg ccggttgaga
       61 gtgctcgctc gggacacagc gggtaacagc ggtgaagcca ccactgacgg ggtattgatc
      121 attcgatgac ccaggtgcca atcgtttcct tcgttgtgct ctgtgagatt tcagtcatct
      181 cacagagctt tttgttttcc tgtccgcacc tgatgtctct caccacaggc tttaaaccac
      241 agatctttca ccagatcagg aagccatgct agagtgtgag gttctcttcc aaccctgaat
      301 tttttcattt tcttatgact gattgggttg aatacccctg aacattgaaa gctaaaaacg
      361 gccctgccgt tgacatagag gagttttttg caatgcgttt gaccaaattt ttgatgaccg
      421 gaatgatatt gatgatcctg attgtcggcg gcttttcgcc agtgctggca acagccgcct
      481 ttcaaaccag cggcccgaat ctggcagtgg ttgaatcaat gaagaccaaa ctgtcactta
      541 cccccgacca ggccaccaaa atcaaagaca tcctgaccaa aagctatgag caatctgaaa
      601 aagaccgggc ttcggcggct ggaaataaag aagcgttcaa aaagctgaac aaaacccgga
      661 aaaaacaaac caaagaacaa atcaaagcag tcctgaccca ggatcaaatc gcgaagtttg
      721 atcaggtcaa gtctgatttg aaatctgcct tgaaaccgaa aaaagcctga gaaccggggc
      781 tgaagaagtc gggctgaaaa acatcgggct gaagaagtcg ggctgaagaa gtcgggctga
      841 agaagtcggg ctgaagaagt cgggctgaag acattgggct cagggtatga aacccacttt
      901 cttcagcccc gagcttgcaa gtcttcagcc ccaagcccca agccctggtt tttgcagccc
      961 cgagcttgcg agtcttcagc ctaagccccc aagccccaaa accagggctc aagatagtgg
     1021 gccggagatt gagtatctcc ggcccatttc ttttttagag caaggggtta ccacctcaga
     1081 agttttgctt gaaaacataa ccctttttga atcagcagga tgtgttttcc ctgttgagtt
     1141 tttggctcat cttgggtaag aaaggaacat acgtttcaat gtgcaggtca aaaactcaaa
     1201 gcgtgttgtg ccgggaactg acacaattga gctggaaaaa tgtatctcat tgattcataa
     1261 gatcttcatt tgattcttta ttcttcgttc tcaactgatt tttaccacaa tgccaaggag
     1321 ggtcggtcag tatgtctgaa ttgcaaagtc caagtttgcg tgaactggaa cactttttac
     1381 gcatttcggt ggaagccaac tactggttga cctttgctga cgtcaatccg gtggcggttg
     1441 tggaagaaat ccaggaacaa ctgatggacc gacggtttga atggctggat ggtgccattt
     1501 acgtccccaa tcttctgacc gtatttgtga ttgagccaac cccggaaaaa cttgaagagc
     1561 ttgaagtcct gtttaactca atggtgttta cgaaatacct gtatgaacat atcaccgagt
     1621 caggctttaa gttgtttgat ttcatgcgca tcgaaattaa ggtcgcaccc actgaccagc
     1681 agcaatttgc cgggccgtgc tacctctttt tcgactggcc aaacgccaat gaatcaaccg
     1741 agcaagtgac ggtcaaatat gacctccagg aacgcaagat tttagagttg tgcgaaccaa
     1801 agccggatat tcctcgcctg gctcgcctga cagcgctcaa tgccgaagtc tatcggaatc
     1861 aatacaccat cacccgttca tcagtttcga taggacgtct gcgcaatgtg attgataaac
     1921 acacaggcca ggtgttgcgt cgcaacgatt ttgtgtttgc ccgccagcag gtgcctgatt
     1981 caccgaatgc gagtgtttcg cgccaacatg gacggatttc ctttgaagat ggagagtttt
     2041 tcttttacga cactggcagc gtcaatggca gcgccatcga acgtgcaggc acgaccattc
     2101 cggttgccac gtcaaccaat ttcctgcaac gggttcagct ccacgaccac gacatcatca
     2161 cgctgggtgc agctcgaatt ttgtttgaag tgattcacac ccccgaagag attggtcggc
     2221 tgcaaaccag cggcgaaacg actcaggaaa gagcagattc cctctatgac ctggaaggtc
     2281 tggaaaccct gcgcgttttt ccgaaagacc ttcgctaaaa tactcaggcg gcccagtaac
     2341 gggccgcctg gtcaagtctt tcgcaacctg ggggatttcc attatcttac tggctgaact
     2401 gcctgccgaa cgacccgaat gcggttattg atctgatcac tgatcaccag atttccaagt
     2461 gaatccacgg caatatcagc cggaccaaaa agttgtgcgg ttgttgccgg acctccgtca
     2521 cccgcgcttc catactgacc gttcccagcc actgtggtaa tcaactgggt ggcagtattg
     2581 accagtcgaa cccgagcatc ctgataatca gcaatcaaca cattgttggc accgtcaatc
     2641 gccagagaaa tcgggaacgt caggctggcg atacttgccg gacgattatc gccgttgaac
     2701 ccggtttcac cgtttccggc cagggttgaa atccgacccg atgggtccac tactcgtact
     2761 cgatgattcc ccgcatccgc aatatagagc ctgccggtac gatcaaatgc cacatcaaac
     2821 ggcgctgaaa gttgagctgt aatggccggg ccgccatccc cactaaaccc ctgaacggct
     2881 gaccgtccaa ccactgtctc gatgatccca gtagccatca ccacgcgccg aactcgattt
     2941 gttccagcct cagcaaaata caggtttcca tctggcccaa aggcagcccc catcggataa
     3001 accagccggg aatctgtcgc tttctgccca tcagataaac tcccatttgg atttccagca
     3061 aaggttgaaa tagtttgagt ggcggcatcc actcgccgga tggcgccatc ttcattgtca
     3121 agaatataaa cattcccctg agcatcagcg acggtccgtg caggcgagac cagggcggct
     3181 ccacgggctg gccccccatc acccgaagct ggtttatcga aatcaaacac accgg
//
LOCUS       DAOQDO010000478         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_22959_length_3235_cov_2.428931, whole genome shotgun sequence.
ACCESSION   DAOQDO010000478 DAOQDO010000000
VERSION     DAOQDO010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22959_length_3235_cov_2.428931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2511
                     /locus_tag="PKZ53_08910"
     CDS             <1..2511
                     /locus_tag="PKZ53_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40596.1"
                     /translation="TISMDGKMIAFVSNGTDFVADQKTLPREKNVFLYNQETGRIDFV
                     SRPQSKPDERFVRDSSAPILNADGKYLLFSSSASDLIPGQNDHNNGSAEDDEGGGSDV
                     FLYEPETGRMCLVSHQSSSLTDTASGESFGTGISADGRQVVFSSYAADLIDGQIDPAR
                     GDRPENEDVFLFDRETQTTILVSHNSYSSLFTGDARSYNPVISADGTSVAFASLATNL
                     VPGQYDPGLGGRKCNLFLFHRLDKSVTLISHSASSPTEVAQNLATLNLDQFSLALNTT
                     GEFVYYATLAFNLEPNLTDLNRNWDLYVFSQLEQTNHLVTFRAVNEAGMIADGSSFVG
                     GGYWGDGSSSAISQTGDVLVFTSTASNLVPGQIDVNRGLTDREDIFLFDRASQTTHLI
                     SHKPGFSGMTGARRSLLPVLSRNGRYVVYLSEASDLVAGLRDGNGAFWDVFLYDRATQ
                     TTDLVSHAVDSLIDTGNSDKGIGPLYQAPSISEDGRYIVFSSTANNLMEGFKNPNRGY
                     EVFLYDRETKVIRLVSHLDGDPLQAGLGNAIDAAISADGRFITFASRSLGLIKGQPPG
                     VSATANLYVYDRVKDITRMVSHIGDSVQTGGNDDSRFPSMSADGRYITYISQATNLIA
                     WQIDKNKKTFDGYDVFVYDQKTRTNQLVSHTQASAITTGDGDSHAPMTSADGRYIVYR
                     SLASNLSKNQIDKRSRAYDIFLYDRKTGQNILVSRSYGTKNTAGNHESFETSLSADGK
                     RLVFQSVATDLVANQPQIFQRSNSNIFLFDVETQTTSLISYSRVSPVQPTNYGASGAA
                     ISPMGDFLVFSSAHTDLVRNDFNRTKDLFLVRLPLNPK"
     gene            2695..>3235
                     /locus_tag="PKZ53_08915"
     CDS             2695..>3235
                     /locus_tag="PKZ53_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40597.1"
                     /translation="MNASRGRCFVLVCLLVVLTGLGLQPVTPVTAAALSIQETPSAPT
                     PIQELQPGTPIERSLKSEESHVYAIHLKAGQFLELSVAEKRVDLLAEFFAPDGKRLVY
                     SDVRAGAGKEIIRLLIDADGVYRLELTAKKLLQPGDYLIEMDVRQPTETDRILIETST
                     NLDEAQKLYEAGLASVKDLI"
BASE COUNT          817 a          758 c          823 g          837 t
ORIGIN      
        1 accatcagta tggatggaaa gatgattgcg tttgtgagca atggcacgga ctttgtcgcg
       61 gatcagaaga ctcttcccag agaaaagaat gtttttttat ataatcagga aactggacgc
      121 attgattttg taagtcgtcc gcaaagcaaa cccgatgaac gatttgtcag agactcgtca
      181 gccccgatac tcaatgctga tggaaaatat ttgttatttt caagcagtgc ctcagatctg
      241 ataccaggcc agaatgatca caacaacggc tcggctgagg atgatgaagg aggggggagc
      301 gatgtgtttt tgtatgagcc ggaaactggc cggatgtgcc tggtcagtca ccagagttcg
      361 tccctcactg acactgccag tggagagtca tttggcaccg gtatcagtgc ggacggaagg
      421 caggtggttt tcagcagtta tgccgctgat ctgattgatg gacaaattga ccctgcgcga
      481 ggggacaggc cggaaaatga ggatgtcttt ctctttgatc gggaaactca aacgaccatc
      541 ctggtaagcc ataattccta ttcatctctt tttactggag atgctcgatc ttataacccg
      601 gtgatcagcg cggacggaac cagcgtggcc ttcgccagtc tagccaccaa tcttgtgcca
      661 gggcagtacg acccgggtct gggaggccgt aaatgtaacc tttttctgtt tcatcgattg
      721 gacaaatctg tcactctgat cagtcattcg gccagttcgc caactgaagt ggcacaaaat
      781 ttggctaccc tgaaccttga ccaattttcc cttgcgctca acaccaccgg agaatttgtt
      841 tattacgcga ccctggcttt taatctcgaa cccaacctca ctgacctgaa ccgcaactgg
      901 gatctgtatg tattttctca acttgagcaa acaaaccatc tggtaacttt ccgggctgtg
      961 aacgaggcgg gaatgatagc ggacgggagt agttttgttg ggggcggata ctggggagat
     1021 gggtcgtcgt ctgcgatcag ccagaccggc gacgttctgg tttttacaag taccgccagc
     1081 aaccttgtac cagggcaaat tgatgtcaat cgtgggttaa ctgacagaga ggatattttt
     1141 ctttttgatc gggcgtcaca gaccacccac ttgattagcc ataagcctgg tttttctggc
     1201 atgactggag cgcgacgatc tctgttgcca gttctgagca gaaatggccg gtatgtggtt
     1261 tacttgagcg aagccagcga tctggtagcc gggctacggg atggaaacgg ggcgttttgg
     1321 gatgtttttc tgtatgaccg ggcaacccaa accacagatc tggtcagtca tgctgttgac
     1381 tcactgattg atactggaaa ctctgataaa ggcattggcc ccctgtacca ggcgccgtca
     1441 atcagtgagg atggcaggta tattgtattt tcgagtactg ccaataatct gatggaagga
     1501 tttaagaacc ccaatcgagg ctacgaggtg tttctctatg atcgggaaac caaagtgatc
     1561 aggttggtga gccacctcga tggagatccg ctccaggctg gtttgggaaa tgcgattgat
     1621 gctgccatca gtgcggatgg tcggtttatt acgtttgcca gccggtcact cggtctgatt
     1681 aaaggtcagc cgcctggggt ttccgccact gccaatctct atgtctatga tcgggtgaag
     1741 gacatcaccc ggatggtcag tcacattggc gattcagttc aaaccggtgg aaacgacgac
     1801 agtcggttcc cctcaatgag cgctgatgga aggtatatta cctatatcag tcaggccacc
     1861 aatctcattg cctggcagat agataagaac aaaaagacat tcgacggata tgatgtgttt
     1921 gtgtatgacc agaaaacaag aaccaaccag cttgtcagtc acactcaagc ctcagccatt
     1981 accaccggag atggcgactc gcatgctcca atgacgagtg ccgatggccg ctatattgta
     2041 tatcgaagtt tagcttccaa cctgagtaag aaccagattg acaaaagaag ccgtgcttac
     2101 gacatatttt tgtatgaccg gaaaaccggt cagaacatcc tggtcagtcg cagctatgga
     2161 accaaaaata ccgctgggaa tcacgaatca tttgaaacct ctctcagtgc tgatgggaaa
     2221 cggcttgttt ttcaaagtgt tgcgactgat ttggttgcca accagccgca gatttttcag
     2281 cgatccaatt caaatatatt tttgtttgat gtggagactc agaccacatc gctcatcagt
     2341 tattctcgcg tttcacccgt tcaacccacc aattacggcg cctccggggc cgccatcagt
     2401 ccaatgggtg attttctggt gttttcaagc gctcatactg atctggtgag aaatgatttc
     2461 aatcgaacaa aagatctgtt tttggtacgg cttcctctca atcccaagta gcgattcaga
     2521 ccagaaaccc aaaagcctgg accagattta gttgattctg aatagttgga atgcgatagt
     2581 ccctccgatc acgacgtctg tgcttcaaac ccaaccctga gtcaagggtt gggtgccccc
     2641 cactactcat ctcaatcctt cattttccct tcattcatat caggagtgtg cccaatgaac
     2701 gccagtcgag gtcgttgctt tgttcttgtc tgtttgctgg tggtgttgac aggtcttgga
     2761 cttcaaccgg tgacgccagt tactgcggcg gcactttcca tccaggaaac gccatctgct
     2821 ccgacaccaa tccaggaact ccagcctggg acaccaattg agcgaagtct taaaagtgaa
     2881 gaatcacacg tctacgcgat tcacctgaaa gccgggcagt ttctggagtt gtcagtcgct
     2941 gaaaaaagag tagatctttt ggctgagttt tttgcccctg atggaaagcg gttggtctac
     3001 agtgatgtac gtgctggggc gggtaaagaa ataatacgcc ttctgattga tgcggacgga
     3061 gtgtatcggc tggaacttac tgccaaaaaa ttactccaac ctggtgacta cttgatcgaa
     3121 atggatgtgc ggcaaccaac cgaaacggat cgaattctga tcgagaccag tacgaatctg
     3181 gatgaagcgc aaaaattgta tgaagcaggt ttagcttcag taaaggactt gatcg
//
LOCUS       DAOQDO010000479         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23005_length_3231_cov_3.076826, whole genome shotgun sequence.
ACCESSION   DAOQDO010000479 DAOQDO010000000
VERSION     DAOQDO010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23005_length_3231_cov_3.076826"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1561
                     /locus_tag="PKZ53_08920"
     CDS             227..1561
                     /locus_tag="PKZ53_08920"
                     /inference="COORDINATES: protein motif:HMM:NF021641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ40598.1"
                     /translation="MPVHLTKGGRVNLSKEAPQLKRVLVGLGWKTSQSGTPYDIDASV
                     FMLGSNGKIPHEEYFVFYNNLRSRDGSVIHSGDNRQGGVSEDAEVIRVELPNVDPTIT
                     ELVFVVTIDGALEKNQSFGQVRDAFIRVVDEDTNRELARFSLSDDSSTETALEFGKLY
                     RKDGDWRFQAVGQGYRSGLQGFVDRYYQEHPTSSASPPPTSAPVAVAPPAPVVSFVPQ
                     VPLPPKVSLLKQKVEVTLARKNLMGVKARVGLSMDITGSMTQLYKNGTVQNVVERILA
                     VALSFDDDGSLDVWRFDHRFKRLAPATVHNFEGYVNREALSDRDIYGHNDEPLVMRDI
                     VQKYTVEEPSLIPAFVVFVSDGGVKKNQEIQEIITNAAQYPIFWQFVGIGNAKFGALE
                     RIDTLPGRIVDNASFFALNDIAAISDEELYDRLLTEFPLWLAAARSKGIVRS"
     gene            1606..3108
                     /locus_tag="PKZ53_08925"
     CDS             1606..3108
                     /locus_tag="PKZ53_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40599.1"
                     /translation="MPKVTIPNTDSLYHLVCFDENGNERTDDEQGKLSEVVLSTLTEK
                     PVTDVFVISHGWKGDIPAAIDQYNLWIKAMADCEIDIDRIRMICPEYHPLLIGLHWPS
                     LPWGNEQLGSQPAESSAVDLGSGNSHQFSADYYPCLMPTDLATEALEIILEYERTHAG
                     SKDKPPREILFACKVLYQESGLIGGGLSAAPGHDHHPFDPLAFYELIEAQVPASEIPE
                     FDNQSPNYGDAFALQAPGWIVSLLQQLSFWKMKERARNFGQSSGFQLLNQLLAAAPEA
                     RFHVMGHSFGCIVASSVVGGPNGTGHLVRPVDTLFLVQGALSLWSYAEDIPYGNGGPG
                     YFHPVVKEQKIKGPLVTTRSKYDSAVGVLYPMASKVSGDACFATADEPKPPEIGALGS
                     YGAHGINSIVVDSPMLPATRLYRFEPGKIYNLEASQYISKIENATSGAHNMIAEPEVA
                     HAFWEAILSVIPGKTSRPQPSLTEPAIPGSNAEPEPVFATKLHPTETGGC"
BASE COUNT          795 a          831 c          826 g          779 t
ORIGIN      
        1 ttcagccccg agcttgcgag tcttcagccc caagccctga gcccgagtat tttcagcccc
       61 gagcttgcga gtcttcagcc ccaagccctg agcccgagta ttttcagccc cgagcttgcg
      121 agtcttcagc cccaagccct gagcccgagt attttcagcc ccaagccctg agcccgagta
      181 ttttcagccc gatgtctttc gctctcaccc tgaaaggagt caacacatgc cagtccattt
      241 gaccaaaggt ggtcgcgtca atctctcgaa agaagctccg caattaaagc gcgtgctggt
      301 tgggcttggt tggaagacaa gccagtctgg aacaccctat gatattgatg cctcagtatt
      361 tatgctggga agcaatggga aaatcccgca cgaggaatat tttgtttttt ataacaatct
      421 ccggtcacgg gatggatctg tcattcactc aggcgacaac cgccagggag gtgtttcgga
      481 agatgccgaa gtgatccggg ttgagcttcc caacgttgac ccaacgatta ccgaactggt
      541 ttttgtggtc acgattgatg gtgcgcttga aaagaaccag agtttcggac aggtccggga
      601 tgccttcatc cgtgtggtgg atgaagacac aaaccgcgag ctggcccggt tttcactcag
      661 tgacgattcg agcacggaaa ccgcgttgga gtttggcaaa ctctatcgca aagacggaga
      721 ctggcgattt caggccgtcg gccagggcta tcggtctgga ttacaaggct ttgtggatcg
      781 ttattaccag gaacacccga cctcatcggc gagtccacca ccaacatcag caccagttgc
      841 cgtggctcca ccagcgccag ttgtttcgtt tgtgccacaa gtgccacttc ccccaaaggt
      901 gagtctactc aaacagaaag tcgaagtgac actggcccgg aaaaacctga tgggtgtgaa
      961 agccagagtt gggctttcaa tggacattac tggttcgatg acccagttgt acaaaaacgg
     1021 aacagtccaa aacgttgtcg aacgaatcct ggccgtggcg ctttcctttg acgatgacgg
     1081 cagccttgat gtctggcggt ttgaccatcg cttcaaacgg ctggccccgg cgacggtcca
     1141 caattttgaa gggtacgtca atcgcgaagc tctctccgac cgggatattt acggccacaa
     1201 cgatgagccg ctggtgatgc gtgatattgt tcaaaaatac acggttgaag aaccgagctt
     1261 gattccagct ttcgtcgttt ttgtgagtga cggaggcgtc aaaaagaatc aggaaatcca
     1321 ggaaatcatt acaaacgcag cacaataccc gatcttctgg caatttgtcg gaatcggaaa
     1381 tgcgaaattt ggcgctttgg aacgcattga tacgctgccg ggccggattg tggacaacgc
     1441 ttcgtttttt gccctcaacg acattgcggc gatttcagat gaagaactct atgaccgatt
     1501 gctcaccgaa ttcccgctct ggctggcggc tgccagaagc aaagggattg tgagatcgta
     1561 acaggctaaa tcttcgataa aatcgtttct caaaggaatc atcccatgcc caaagtcact
     1621 attcctaata cagacagttt gtaccatctg gtgtgttttg atgaaaatgg caatgaacgc
     1681 actgatgatg aacaggggaa actaagcgaa gttgtgctca gtaccctgac tgaaaaaccg
     1741 gtcaccgatg tatttgtcat cagtcacggc tggaaaggtg acattcctgc cgctattgac
     1801 cagtataacc tgtggattaa agccatggcg gattgcgaaa ttgacattga tcgcattcgg
     1861 atgatttgtc cagaatatca tccattgctg atagggttgc attggccaag tcttccctgg
     1921 gggaatgagc aacttggatc acaacctgca gaaagcagcg cggttgatct gggttcaggg
     1981 aatagccatc aattttcagc cgactactat ccttgtttaa tgccaactga ccttgccacc
     2041 gaagcacttg aaatcatttt agagtatgag cgcacccacg ctggctcaaa agacaagccg
     2101 ccacgtgaaa ttttatttgc ctgcaaagtg ctgtaccagg aatctgggtt gatcggaggt
     2161 ggattgagtg ctgctcctgg acatgaccat cacccctttg acccattggc tttttatgaa
     2221 ctgatcgaag cacaggttcc cgcttcggaa attcctgaat ttgataacca gtcaccaaat
     2281 tatggagatg cgtttgcttt acaggcgcca ggttggatcg tgagtttgct ccaacagctt
     2341 tcgttttgga agatgaaaga acgagcccgc aattttggac aatccagcgg gtttcaattg
     2401 ttgaaccagt tgcttgcagc agctcccgaa gctagatttc acgtgatggg gcacagcttt
     2461 ggttgtatcg tcgcttcctc agttgttggt ggcccaaacg gaaccgggca tttggtcaga
     2521 ccggtggaca ctctgttttt agttcaagga gcattgtcct tgtggtccta cgccgaggac
     2581 attccctatg gcaatggtgg tccggggtac ttccacccgg tggtcaaaga gcaaaaaatc
     2641 aaaggtccac tggtcaccac tcgttcaaaa tatgattcag cggtcggagt cctctacccg
     2701 atggcctcaa aagtcagtgg cgacgcctgt tttgcgacgg ctgatgaacc caaaccgcct
     2761 gaaatcgggg cgcttggttc gtacggtgcc cacggtatca actcaattgt ggtggacagc
     2821 ccgatgttgc cagcgacgcg gctctatcgg tttgaaccag ggaaaatcta caacctggaa
     2881 gctagccagt acatttcaaa aattgagaat gcaacttcag gtgcgcataa catgattgcc
     2941 gagccagaag ttgctcatgc cttttgggaa gccatcctgt cagtgattcc aggcaaaaca
     3001 agccgtccac agcccagtct gaccgagcca gccatccctg gctccaacgc ggaacctgaa
     3061 ccagtatttg caaccaaact acacccaacg gaaacagggg ggtgctgagt tcacggcgac
     3121 ttcaccagag caaatttcat caattgaacc tccctcccta ccgctgtgcg gttgaggcgg
     3181 gggattcttc ctgttccgga caaccgcttc cgttccggct cgacgccgaa c
//
LOCUS       DAOQDO010000480         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23023_length_3230_cov_2.796850, whole genome shotgun sequence.
ACCESSION   DAOQDO010000480 DAOQDO010000000
VERSION     DAOQDO010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23023_length_3230_cov_2.796850"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKZ53_08930"
     CDS             complement(<1..720)
                     /locus_tag="PKZ53_08930"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNJ40600.1"
                     /translation="MSSTISLSKTQKLAGTVVLPGDKSISHRLGMLASLADGTTHITN
                     FATSEDCRSTLNCMQALGVSVEGTDSETNSIAIEGRGLNGLQPPKDILDARNSGTTIR
                     LLSGILAGQPFVSTITGDESLSQRPMRRIMTPLTAMGARIEATNDQFAPLTIQGGALH
                     PIQYTLPMASAQVKSCVLLAGLYADGETSVIEPVPTRDHTERMLRAFGVNLTTDGKEI
                     RIQGGQQLKAPPTSCTVPSDFS"
     gene            complement(1081..>3230)
                     /locus_tag="PKZ53_08935"
     CDS             complement(1081..>3230)
                     /locus_tag="PKZ53_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HNJ40601.1"
                     /translation="YPELAPFKSKSIGTLAEVLPDKIDTRLARLIGYFVAEGCYLKQN
                     DQKDRGILITNQNPQVIADLKEICTAYGLKMSSDAKGHHALSHAGLATLFHQFGFDGR
                     RAHDKHIPQIIRRSPKEIMSAFLRAYFDGDGTVTTYISCSTASERLAEQLQIVLLNYG
                     IISKCQRHFNEKTQRTYYMIQIDSENIAIFNREIGFGIDYKRELAEKLEAEDHIAHRD
                     TVPNLQTHWSQVQQELQVAAKAAVAAAGLGYRDRFGARQLIGDMNYEQLWRFAKGKSA
                     PSYQIAKTIAEVASEHVTVPEPIFSALESQYFFDEVEEKTEGRCDLWDFHVPEEHSYV
                     GNGFVNHNCDEKFLYSLLTLSQEQSIKTGRYAMIYADEVIVSHTNENEYQSFVGNKKN
                     EALQDRIIMIRVPYNLKVSEEERIYKKLLQQSELRQVHIAPYTLHVAAIFAVLTRLEP
                     PKRANVDILKKMKLYDGEDVEGFKSKDVRELKAETVREGMFGISPRYIINRLSNALIK
                     EGHKYITAIDALRAIRDGFDQHTGINTEERERYLNLITMARKEYDEIAKLEVQRAFVY
                     SFEEMAKSICDNYLDNVEAYCNKEKIKDALTEEELEPDEKLMRSIEEQIGISENAKNT
                     FRQEILIRISTYARKGKRFEYNSHERLKEAIEKKIFSDLKDVVKITTSAKSPDPDQLR
                     RINEVIDRLVREHGYTTESANDLLNYVGTLLSR"
BASE COUNT          718 a          811 c          818 g          883 t
ORIGIN      
        1 cgaaaagtct gatggcaccg tgcaactggt cggcggtgct ttcaactgct gaccaccctg
       61 gattcggatt tccttcccgt ctgtggtcag gttgactcca aaggcacgca gcatgcgctc
      121 agtgtggtca cgcgttggaa ctggttcaat caccgatgtt tcaccgtcgg cataaagtcc
      181 ggccagcaag acacacgact tcacctgggc actggccatg ggcaaggtgt attgaattgg
      241 atgcaatgca ccaccctgaa ttgtcaatgg ggcaaactgg tcattggtcg cctcgatccg
      301 ggcacccatc gcggtcagcg gcgtcataat gcgccgcatc ggacgttgtg acagtgattc
      361 atcacccgtg atggtactga caaacggctg cccagccaga attcctgaca gaagccggat
      421 ggtcgtcccc gaattacgcg catccaggat gtctttcgga ggctgtagcc cgttgagccc
      481 tctaccctca atcgcaatcg agttcgtttc tgaatccgtc ccctcaactg agactccaag
      541 tgcctgcata cagttgagcg tgctgcggca atcttcactg gttgcaaaat tggtgatatg
      601 cgttgtgcca tcagccagtg atgccagcat gccaaggcga tgggaaatgg atttgtctcc
      661 gggcagcacc accgttccag ccagtttttg tgtttttgaa agtgaaatgg ttgaagacat
      721 atttgattga gggttcagga ttcggggttg aaaaccaggg ttcagggttt aagcatcagg
      781 acaagggttt taggcccgaa gggttgtcgt ttaatagccg tggtgcgaag cccacggtct
      841 ctgagaaaaa acaatggggc gaagcgtgtt cgccccaaga gatcaagctc cggtttgcca
      901 gtcgttgccc cataagcgcc aggataagaa aaactgtgtt ccttccgttg gcattaaaca
      961 atgttctgtt agggattgga tctcgtcctg gctgtttccg tgggcttcgc accacggcta
     1021 ttcaacgacg accctttggg cctcaaatcc ttcccctagc gcttatggtc gttgccctcc
     1081 ctaccgcgac aacaatgtgc cgacgtagtt gagcaaatca ttggcgcttt ccgtcgtgta
     1141 gccatgttca cgcaccagcc ggtcaatcac ttcgttaatc cgccggagtt gatccggatc
     1201 gggggatttg gccgaagtcg tgatcttgac cacgtctttg aggtcggaaa agattttctt
     1261 ctcgatggct tctttgagcc gttcgtgtga attgtattca aaccgtttgc ctttgcgggc
     1321 ataggtcgaa atgcgaatca ggatttcctg acggaaggtg ttcttggcgt tttctgaaat
     1381 cccgatttgc tcttcaatcg agcgcatcag tttttcatcc ggttcaagtt cctcttcggt
     1441 cagggcgtct ttgatctttt ccttgttgca gtaggcttcg acgttgtcca gatagttgtc
     1501 acaaatgctc ttggccattt cttcaaacga atagacaaac gcccgctgga cttcgagctt
     1561 ggcaatttcg tcatattctt tgcgcgccat ggtgatgagg tttaaatatc gttcgcgctc
     1621 ttcggtattg atgccggtgt gctgatcaaa cccgtcccgg atggcccgca gggcatcaat
     1681 ggccgtgatg tacttgtgtc cttccttgat caatgcattc gagagccggt tgatgatgta
     1741 gcgtggggaa attccaaaca ttccctcccg caccgtttct gcctttaact ctctgacatc
     1801 cttgctctta aagccttcga cgtcttcacc gtcataaagc ttcattttct tcagaatgtc
     1861 cacgttggcg cgtttgggtg gttcaagccg ggtcagaacg gcaaaaattg ccgcgacgtg
     1921 caacgtgtac ggagcaatat ggacttgacg gagttcgctc tgctgcaaga gcttcttata
     1981 gattcgctct tcttcggaaa ccttcaggtt atagggcacg cggatcatga tgatgcgatc
     2041 ctggagcgcc tcgttcttct tattgccgac aaatgactgg tattcgtttt cgttggtgtg
     2101 cgagacgatg acttcatcgg cataaatcat cgcgtagcgg cccgtcttga ttgattgttc
     2161 ctgtgacagc gtcagcaacg aatagaggaa tttctcgtcg cagttgtgat tgacaaagcc
     2221 gttgccgaca tacgaatgct cttcgggaac gtggaaatcc cacaaatcac accggccttc
     2281 ggtcttttct tcaacttcat caaagaaata ctgggattcg agcgcggaaa aaatcggttc
     2341 gggaacggtc acatgttcgc tggctacttc ggcaatggtt ttggcgattt gatatgacgg
     2401 agcagacttg cctttggcaa agcgccagag ttgctcgtaa ttcatatctc cgatcaactg
     2461 ccgggcgcca aaccggtcac gatacccaag ccccgccgct gccaccgccg ctttggctgc
     2521 cacctgtaat tcctgttgca cctggctcca gtgagtttgc aaatttggaa ccgtgtcgcg
     2581 atgggcaata tggtcttccg cttccagctt ttcagccagc tcgcgtttgt aatcaatccc
     2641 aaagccgatt tcccgattga aaatagcaat gttttcgctg tcaatctgga tcatgtagta
     2701 agtgcgctgg gtcttttcat taaaatgccg ctgacacttg ctgatgattc catagttgag
     2761 caacacaatt tgaagctgtt cggcaagccg ttcggaggcg gtactgcaag aaatgtatgt
     2821 ggtaaccgtt ccatcaccat caaaatatgc ccgcaaaaaa gccgacatga tttctttcgg
     2881 cgaacgacgg atgatctggg ggatgtgttt gtcgtgtgcc cggcgaccat cgaagccaaa
     2941 ctgatggaat aaggtagcta acccggcatg actcagggca tggtgacctt tggcatcaga
     3001 actcattttc aagccatagg cagtacagat ttctttcaaa tcagcaatca cttgaggatt
     3061 ctgattggta atcaaaattc ctctgtcttt ttgatcattt tgcttcaggt agcaaccttc
     3121 agctacaaag taaccaatta agcgtgccaa cctggtatca attttatctg gcaaaacttc
     3181 tgccaatgtt ccaattgatt ttgatttgaa tggagccaac tcagggtagg
//
LOCUS       DAOQDO010000481         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23076_length_3226_cov_3.744560, whole genome shotgun sequence.
ACCESSION   DAOQDO010000481 DAOQDO010000000
VERSION     DAOQDO010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23076_length_3226_cov_3.744560"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKZ53_08940"
     CDS             complement(<1..292)
                     /locus_tag="PKZ53_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007428141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNJ40602.1"
                     /translation="MAGLMKRVIPCLDVDRGRVVKGTKFLNLQDMGDPAELAVAYEHE
                     GADEIVMLDISASVENRSAAHATIRAIASQLSIPFTVGGGISTLTDIYDVLTS"
     gene            complement(280..852)
                     /gene="hisH"
                     /locus_tag="PKZ53_08945"
     CDS             complement(280..852)
                     /gene="hisH"
                     /locus_tag="PKZ53_08945"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNJ40603.1"
                     /translation="MPIAIINTNAGNVTSLIHAMTRLGKETRLLTSPDDLSDVSQLVI
                     PGQGHFGSVMDHLTQTGWVAAIHKWVTEDKPLLGICVGLQILFEESEEAPGVAGLGIF
                     SGRVTRLRSPKQPMVGWAEVNWSGDLFPNGAAYFVNSFVVRDAADCVAQTTYGEQFCA
                     VVERGSVIAFQFHPEKSGDYGRRIFEQWLV"
     gene            complement(1021..1593)
                     /gene="hisB"
                     /locus_tag="PKZ53_08950"
     CDS             complement(1021..1593)
                     /gene="hisB"
                     /locus_tag="PKZ53_08950"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNJ40604.1"
                     /translation="MNITRQRSTKETTIAVELNVHGTQQVTIDTGLPFFNHMLEQLGT
                     HAGWNLKIVASGDIKVDDHHLIEDVALCLGDALQEAWRTRQRIQRYGQRALPMDEVLV
                     LCALDLSGRPYCKCDLPFTREYVGHLNTEMWSHFFHSLAMAGAFTLHIQTLATGNNHH
                     LVEASFKALAYALSEALQPKDREVSTKGVL"
     gene            complement(1727..2734)
                     /locus_tag="PKZ53_08955"
     CDS             complement(1727..2734)
                     /locus_tag="PKZ53_08955"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNJ40605.1"
                     /translation="MSILKLDTNERADGCPQWVASAFTQLDSKSLWLYPNRVPLEEKL
                     ARKFGLEPSQVLVTNGGDEGIALLARLIRNQNRRVLIPLPAFSIYFKCLGEVSAEIAT
                     TPALPNLGIDYPCLIKTLQEGTFGWLILTSPNNPTGEVIPRPVLLELLQIAAQKGVFV
                     LLDEAYVDFYPENAAGLLAEFSNLVLLRTFSKAYGLAGLRVGFLLGASELMNQVRALT
                     LPYNVGTPALHLASAGLESEAEADTLAYCRQIMRNRKQFQNWLQGRDIEVFDSGANFL
                     MIRLSPERARQCRDSLREQGIIVRLFDDPLLAGCIRITIPLDLERLTTALADASLFRR
                     P"
     gene            complement(2958..>3226)
                     /locus_tag="PKZ53_08960"
     CDS             complement(2958..>3226)
                     /locus_tag="PKZ53_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40606.1"
                     /translation="NDTTRLNFGLAFTSDGFRDQFINPGLINTGGIDVSGAIYKKILG
                     QWFNVGIAGLIGRERIVEPQVNPAFSGAYRGHGIMISLGMRLRK"
BASE COUNT          762 a          853 c          883 g          728 t
ORIGIN      
        1 cggaggtgag cacgtcatag atgtcagtga gggtgctgat gcctccgcca accgtgaagg
       61 gaatcgaaag ctggctggca atggcccgaa tcgtcgcatg cgctgccgac cgattttcga
      121 cgctggctga aatatcaagc atcacgattt cgtcggcgcc ctcgtgttca taagccaccg
      181 ccagttccgc cgggtcgccc atatcctgca aattcaaaaa ttttgtgcct ttgaccacgc
      241 gtccgcgatc aacatcgagg cagggaatca ctcgtttcat tagaccagcc attgttcaaa
      301 gatcctccgg ccatagtcgc ctgatttttc agggtgaaat tgaaacgcga tgaccgaccc
      361 acgctccacc acggcgcaaa attgctcacc gtacgttgtt tgggcgacac aatcagccgc
      421 gtcacgaacc acaaaactgt tcacaaaata agccgcacca tttggaaaga ggtctcccga
      481 ccagttgact tctgcccagc cgaccattgg ttgtttgggg ctgcgcaggc gagtgacccg
      541 acctgaaaag atacccagtc ccgcgacacc aggggcttct tcgctttctt caaacaaaat
      601 ctggagaccg acacaaatcc ccaaaagcgg tttgtcttcg gtcacccact tatgaatggc
      661 cgcgacccag ccagtttggg tgaggtgatc cataacactc ccaaaatggc cttgaccggg
      721 aatgacaagc tggctgacat cactgaggtc atcagggctg gtcagcaacc gggtctcttt
      781 cccaagtcga gtcatggcgt gaatcaggct ggttacattt ccagcgttgg tgttgatgat
      841 ggcaataggc ataaatttag ggctgaagat gacgggcttg gggctgaaga ctcgcaagct
      901 cggggctgaa gatgacgggc ttggggctga agacgacagg cttgggattg aagaaaacgg
      961 gttcaatacc ctgagcccga tgtcttcagc cctcagcccc gagcctgcga gtcttcagcc
     1021 ctataaaact cccttggtgc tgacttcgcg gtctttgggt tggagggctt cactcaaggc
     1081 ataggccaga gctttaaagc tggcttcaac cagatgatgg ttgttgccag tggcaagtgt
     1141 ttgaatatgg agggtaaagg caccagccat ggccagcgag tggaaaaaat gactccacat
     1201 ttcggtgttg agatggccga catattcgcg agtaaacggt aaatcacact tgcaataagg
     1261 tcggccactt aaatcaagcg cacacagaac cagcacttca tccatcggga gtgcccgctg
     1321 gccatagcgt tgaatccgtt gccgggtccg ccaggcttcc tggagcgcat cgcccagaca
     1381 caacgcaacg tcttcaatta agtgatgatc gtccactttg atgtcaccgc tggccacgat
     1441 cttgagattc cagccagcgt gggtgccgag ttgttcgagc atgtggttga aaaacggcag
     1501 gcccgtgtca atcgtgacct gctgggtgcc gtggacgttg agttcaacgg caatggtggt
     1561 ttctttggtg cttcgttggc gagtgatgtt cataaaaccg ttcaggggtc agggttccgg
     1621 gttccgggtt ccgggttccg ggctccgggt tccgagttcc gagttttcga agacaggttg
     1681 tttttggttt tcgccgcgga gcggtggctg aattgaggca ggtcgttcac ggcctgcgaa
     1741 aaagggatgc gtcggccaga gctgtcgtca atcgttccag gtcaagtgga atcgtgatgc
     1801 ggatacatcc cgccagcagt ggatcatcaa agagccggac gatgatgccc tgttcgcgca
     1861 aactgtcacg gcactggcgg gctcgttcag gtgagagtcg aatcatcagg aaattcgcgc
     1921 cgctgtcaaa aacctcgata tctcgaccct ggagccaatt ctgaaactgt ttgcggttgc
     1981 gcataatctg gcggcaatag gccagggtgt cagcttcggc ttctgattcc agtccagcgc
     2041 tcgccaggtg caatgctggc gtcccaacat tgtagggcag tgtgagcgcc cgaacctgat
     2101 tcatcaactc gctcgctccg agcaaaaaac cgacgcgcaa cccggccaat ccataggctt
     2161 tggagaaagt tcggagcaac acgaggttgg aaaattcagc aagcaatcca gccgcattct
     2221 ccgggtagaa gtccacataa gcttcatcaa gcaggacaaa cacacctttt tgagccgcaa
     2281 tctggagcaa ctcaagcaaa actggacgtg ggatgacttc accggtcgga ttgtttgggc
     2341 tggtcaggat caaccagcca aacgtgccct cctgcaaggt tttgatcagg cacggatagt
     2401 caattcccaa attcgggagt gccggcgtgg tggcaatctc ggcagagact tcacccaggc
     2461 atttaaagta gatactaaat gccggcaacg ggatcaagac ccttcgattc tggttgcgga
     2521 tcaatcgagc caggagcgca atcccttcgt ctccgccatt ggtgacgagc acctgggatg
     2581 gttcaagtcc aaatttccgc gccagttttt cttcaagcgg cactcgattt ggatagagcc
     2641 acaggctttt tgaatcaagt tgggtgaatg ctgaggcgac ccactggggg catccgtcag
     2701 cgcgttcgtt ggtgtcaagt ttgaggatag acataagcag aaatagggct gaagacatcg
     2761 ggctgaagac atcgggctga agacatcggg ctgaagacat cgggttgaag acatcggact
     2821 cagggtattg aacccgtttt cttcagccct gagcttgcga atcttcagcc ccaagccctg
     2881 agcttgcgag tcttcagccc taagcttgcg agtcttcagc cccaagccct gagccctggt
     2941 attttcagtc ccgatggtta tttgcgcagg cgcatcccaa gactgatcat aatgccatgt
     3001 cctcggtacg ctccgctaaa tgccgggttt acctgaggtt cgacaatccg ttcacggcca
     3061 atgagacccg cgatgccaac attgaaccat tgtcccaaaa tctttttgta aatggcgccg
     3121 ctgacatcaa tgccaccagt attaatcaat ccaggattga taaactgatc acggaatcca
     3181 tctgaggtaa acgccaaccc aaaattcagg cgcgtggtat cgttga
//
LOCUS       DAOQDO010000482         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23099_length_3224_cov_3.504891, whole genome shotgun sequence.
ACCESSION   DAOQDO010000482 DAOQDO010000000
VERSION     DAOQDO010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23099_length_3224_cov_3.504891"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="PKZ53_08965"
     CDS             <1..1726
                     /locus_tag="PKZ53_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40607.1"
                     /translation="HTIFLPAGDPTRACVQVWQFAPNGAVIQRIVLEDPTSKLFFAFP
                     SLAVNKNNDVLIGYSRFSPDQFASANFVFRGGTDPLNTLRDDTVLKNGEDVYIKSGGG
                     ENRWGDYSSTVVDPVNDLDMWTVQEFAAKQASNGSKWGTWWGQIPAPVSGGDTVAPLV
                     EVTTPNGGEPVKGGDRLRIAWTSSDNLAVVLHDVALSTDGGTTFTTSIATGLPGTAGE
                     FTWAVPLIEVPKARIRVSAFDQFFNQAADTSNADFTIARPPLAPPTNLNATVCGQIVA
                     LNWEAAANTPGAILTGYNVYRSQTSPVSTLPLNRLGSFSAQTRAFTDRPPIDGQNRYF
                     YAVTSVFNTGESGPSNEISASPDGRSDTESPKVVLVSPNGGEVVEAGGSLQIQWEADD
                     DLCLTSQNIDLSTDSGTTFTRTVATGLAGTARTFAFQVPTALDSTTARIRVIARDASG
                     KTGQDVSERDFTIRINDTIAPTVTITSPSANQKVKLRSTTPFSVTWNSSDNIGITSHD
                     VLLARDSAAPFQSVATGLGGTTRSFSLTSAIVGSAKSKTARIRVVTRDTVGNVGMGES
                     APFRINSK"
     gene            1980..3155
                     /locus_tag="PKZ53_08970"
     CDS             1980..3155
                     /locus_tag="PKZ53_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40608.1"
                     /translation="MKCSKCGVDNNLQDRTASNGKCKSCQHPFTFDPKQPTALSTSDT
                     KLHFTDPFFQHALAAITANESLFFTVKQFYYLMNQRFIRKTHTETGCLVSGGIVAGTF
                     ILVFFFSGLAKALVPILIVLAIFALIGFNLYWRTPYVKRKFFSNRKQRDLPAAPKLVK
                     GWLDVWARNNGPIAKLLPPPPPPTHIIAPASVNPEVTVYSFDRLIVTDTPVMAQFLLA
                     NNFHFENNCAVLCIDKYPYDLFWTVMKMLRRNPELKVYAVHDASPFGVHLVHRLRTEA
                     DWFANQPNVTIYDLGILPRQVLDRPVFVQQSPESAQAGRNIAQPVLAALDVTEIQWLR
                     DGYFVELESFAPQALLRMITMGIARSRDPRASDSLVPLDQDPGYTGTTMVYVSDSFG"
BASE COUNT          749 a          835 c          826 g          814 t
ORIGIN      
        1 ccacacgatt ttcctgccgg ctggtgatcc gactcgggcc tgtgttcagg tctggcaatt
       61 tgctccaaac ggtgctgtga tccagcgaat cgttttggaa gacccaacca gcaagctgtt
      121 ttttgccttt ccatcgctgg cagtgaataa aaacaatgat gtgctgatcg gatattcgcg
      181 gttttcccca gatcagtttg cgagtgcaaa ttttgtcttt cggggtggga ctgacccact
      241 caacaccttg cgggacgata ctgttctgaa aaatggtgaa gatgtctata tcaagtctgg
      301 tggaggcgag aaccgctggg gggattacag cagcacggtg gtggatccgg tcaatgatct
      361 ggatatgtgg actgttcagg aatttgccgc caaacaagcc agtaatggca gcaagtgggg
      421 cacctggtgg ggccaaatcc cggcgccagt gagtggcggt gacactgttg cccccctggt
      481 cgaagtgacc acacccaatg gcggtgaacc cgtcaaaggt ggtgaccggc tgcgcattgc
      541 ctggacgtca agcgataatc tggcggttgt gctccatgat gttgcccttt caaccgatgg
      601 cggaaccacg ttcacgacca gcattgcaac ggggttgcca gggacagccg gcgaatttac
      661 ctgggcggtg ccgttaatcg aagtcccaaa ggcccggatt cgagtgtcag cttttgatca
      721 attttttaat caggccgctg acaccagcaa tgctgacttt acaattgccc gtccgccatt
      781 agcaccacca accaatttga atgccaccgt ttgtggtcag attgttgcat taaactggga
      841 agcagcggcc aatacaccag gcgcaatttt aaccggatac aacgtgtacc ggtcgcaaac
      901 ttcacccgtt tcaaccctgc cgctcaaccg tctggggagt tttagtgctc aaaccagagc
      961 atttactgac cgcccaccga ttgatggtca gaaccgatat ttttatgcgg tcacctcagt
     1021 gtttaacacc ggagaaagcg gcccttccaa cgaaatctca gccagccctg atggtcgcag
     1081 tgatactgaa tcaccaaagg tcgtcctggt cagtccaaat ggtggagaag tggttgaggc
     1141 cggcgggtca ctccaaattc agtgggaagc ggacgacgat ttgtgcctta cgtcgcaaaa
     1201 tattgatctt tcgaccgata gtggtacgac ctttacccgg actgtggcca cgggattggc
     1261 tggaactgca cgcacctttg cctttcaggt gccaactgcg cttgattcga ccaccgcacg
     1321 catccgggtg attgcccgtg atgccagcgg aaaaaccggg caggatgtgt cggagcggga
     1381 tttcacgatc aggatcaatg acaccattgc cccaaccgtt acaattacat ctccgagtgc
     1441 caatcaaaag gtgaagcttc gctcgaccac tccattttca gtgacctgga attcatcgga
     1501 caatattggg atcaccagtc acgatgtgtt gctggcacgg gatagcgcag ccccgttcca
     1561 atctgtggcc acgggtttgg gcggcacaac tcgttcattc tcattgacat cggctattgt
     1621 cggaagtgca aagagtaaaa ccgcccgcat ccgtgtggtg accagggaca cggttgggaa
     1681 tgtcggaatg ggtgagagcg ctccattccg aatcaattcg aaataaagaa ttcagggttc
     1741 agggttcagg gttcagggtt caattatcct gtaaccctga aatgggcagc atgcctgagg
     1801 gtacggttgc ccttttttct ttggtgtggg aaagtaagaa cacctccctt ttcctattta
     1861 ccataagccc ctgaaatcta attccgaagc tactgccttg cataaccagg aagttaagtg
     1921 attcactcaa caaagtttga tgaattgctg atgacgaatg ataaggctga tctcacttta
     1981 tgaaatgcag caaatgcggc gttgacaaca atttgcagga ccgaacggct tcaaacggga
     2041 aatgcaagtc ctgtcagcat ccctttacgt ttgaccctaa acagccaacg gcgctgagca
     2101 cttctgacac gaagcttcat tttactgatc cattttttca acatgccctg gctgcaatta
     2161 ctgccaacga gtcgcttttt tttacggtta agcagtttta ttacctgatg aatcagcggt
     2221 ttatccggaa aacgcatact gaaactggct gccttgtcag tggcggaatc gtggctggta
     2281 ccttcatttt ggtatttttt ttcagtggtt tagccaaagc gctggttccc attttgatcg
     2341 tgctggcaat ttttgctctg attggattca acctgtactg gaggactccc tacgtcaaac
     2401 ggaagttctt ttcaaaccgg aaacaacgtg accttcccgc cgcaccaaag ctggtcaaag
     2461 gctggcttga tgtctgggcg cgaaacaacg gtccgatagc aaaattactt ccgccgccgc
     2521 cacctccgac acacattatc gctccggctt cggtgaatcc cgaagtaacg gtctatagct
     2581 ttgaccgcct gattgtgacc gatactccgg tgatggctca gtttttactg gcgaataact
     2641 ttcattttga aaacaactgc gcagtgttgt gtattgataa atacccgtac gacctgttct
     2701 ggacggtgat gaaaatgctc cgccgcaacc cggaactcaa agtgtatgcc gtccacgacg
     2761 cctcgccttt tggggttcat ctggttcacc ggcttcgcac agaagctgac tggtttgcca
     2821 atcaaccaaa tgtcacaatc tatgatctgg gaattttgcc ccgccaggtg ctggatcggc
     2881 ccgtgtttgt tcagcagtca cctgaatcgg ctcaggctgg tcgcaatatc gctcagccgg
     2941 tactggccgc acttgatgtc actgaaatcc aatggctgcg ggacgggtat tttgttgaat
     3001 tggaatcatt tgcaccgcag gcactcctgc ggatgattac aatgggcatt gcccggagcc
     3061 gtgacccgcg agccagtgat tccttagttc cacttgacca ggatcccggt tacaccggca
     3121 caacgatggt ctatgtatcg gattcgtttg gataaaaccc cacccgctca cgcaggtggt
     3181 actgactttt catcctgtct tcggcgtgga gacccggtcc ttca
//
LOCUS       DAOQDO010000483         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23116_length_3223_cov_2.407513, whole genome shotgun sequence.
ACCESSION   DAOQDO010000483 DAOQDO010000000
VERSION     DAOQDO010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23116_length_3223_cov_2.407513"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2717)
                     /locus_tag="PKZ53_08975"
     CDS             complement(<1..2717)
                     /locus_tag="PKZ53_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40609.1"
                     /translation="MRTLFLMICLSLVGNTLMAGWPVLAQSPSEPTSISSLNQHQWGA
                     VALFHGLPSDQVRAIAQDQEGVLWFGTDNGLARFDGRRTQKITAAGVPSSRVSCLQPD
                     PDGSLWIGSESGLYRFQGDRCELVARSAGLTIRAIRLLKNGQVAVACEQGTLLQIRKR
                     GNSEFEVISFSASTYPLLHRERDPDSLLPLTSLGEVDGKLVIGTGGRGVLLVSGTQLQ
                     PLTNQPRAYFVNAVGQDSAGAFWFGGEAPARDSGLLEGPDLKQAQRVPLQTGPVTALA
                     FGDTGETWVGTENQGVFHLRGRELIEHLTFENTAGGLRSNRINAVFLDREGVAWFGTD
                     RGICRYDRFSPAVEAISSDPESNFIRVVLQATDGRIWCGTNRGLFVRSLDQTTWQPVD
                     ACRNRAIHALSQESPEVVLVGTSSGLFQLNAQSLAVLPVEMVRDEVPTLPGSESCRTI
                     GRFRGKLYLAIYGRGVEVIEENRRSLVWPKAAEPDVFRKVTSLLVDGEQRLLIGTAES
                     GIFEYDGTGVKPMANARSLLGKAIWEMMRDRQGILWVATEKGLFAAGDAEFVRVGAEI
                     DVRSVIADPTGSGLWCATVGSGALYWKKKTTGEMVFSLLDRERGLPSDNVFALHLAQQ
                     PDGTQLMWVGTNRGLARYTPLDRSPVLRVVRALGAKTYTASEVLAGIKLEYPQNSLLI
                     EVGASSSRTFPEQFQYEFVARDAAGRVIQTGQSSDGQLSLVNLTPGKYSLEVVAIGND
                     RVASGPFPLAFEVMKSPFPLVTVSLGVLLLLSIGALWWGYRQNRQLAETNNAVANANR
                     QLAETRLQLVTETETERRRIARDLHDQTLADLRRLQLMTDKLPVPAQETGDVQVIPRT
                     FRHEIESISMEIRRICEDLSPSVLSNVGLAAALEWALGTAAAQLPESSR"
     gene            2988..>3223
                     /locus_tag="PKZ53_08980"
     CDS             2988..>3223
                     /locus_tag="PKZ53_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ40610.1"
                     /translation="MAHLPRPSSAPLILIVEDDNSIRFMLKSFLKREGYGVLEARHGQ
                     EALEIFEAHRPDMVLLDLMMPVLDGFQTCSRLRQL"
BASE COUNT          754 a          975 c          806 g          688 t
ORIGIN      
        1 aaccgcgaac tttccgggag ctgggccgcc gccgttccga gcgcccactc cagcgcggca
       61 gccagcccga cattgcttaa caccgaaggg ctcaaatctt cgcaaatccg ccggatttcc
      121 atcgaaatgg attcaatctc gtggcggaat gtccgtggaa tcacctgtac atcgcccgtt
      181 tcctgggcgg ggaccggcaa tttatcggtc atcaactgca gccggcgcaa atcagccagg
      241 gtttgatcgt gcaaatcacg cgcaatccgg cggcgttcgg tttcggtttc agtcacaagc
      301 tgcagccggg tttcggccag ttgccggttg gcgttggcca cggcgttgtt ggtttcggcc
      361 agttgccgat tctggcgata cccccaccag agcgcgccaa tcgagagcaa taacaaaacg
      421 ccaagcgaaa ccgtcaccag cggaaatggc gacttcatca cttcaaacgc cagcgggaat
      481 ggcccggatg ccacccggtc attgccgatg gccaccacct caagggaata ttttcctggc
      541 gtcaggttca ccagcgagag ttgcccatct gatgactgac cggtttgaat cacccgtccg
      601 gcggcatcac gagcgacaaa ttcatactga aactgctcgg gaaacgtgcg gctgcttgaa
      661 gctccaacct caattaacag actgttttgc ggatattcga gcttgattcc ggccagaact
      721 tcgctcgccg tgtaggtttt ggctcccagt gcccgcacca ctcgcaatac cggtgaccga
      781 tcaagcggcg tgtagcgcgc cagtccccga ttggttccta cccacatcag ttgggttcca
      841 tccggttgct gggctaaatg caacgcaaac acattgtctg aaggcagacc gcgttcacga
      901 tccagaagag aaaaaaccat ttctcccgtg gtcttctttt tccagtacaa cgcgccactt
      961 ccgaccgtag cgcaccacag accggatccg gttggatctg caatcacact gcgaacatca
     1021 atctcggcgc cgacccggac aaattcagcg tccccagccg caaacagtcc tttttcagtt
     1081 gcgacccaca agatcccctg ccggtcccgc atcatttccc agattgcttt tcccagcaat
     1141 gatctggcat tggccatggg tttgacacca gtgccgtcat attcaaatat cccggattca
     1201 gccgttccga tcaggagtcg ctgctcgcca tcaaccagca ggctggtcac tttccgaaag
     1261 acatctggct ccgctgcttt tggccagacg aggcttcggc gattctcttc gatcacctct
     1321 acaccacggc catagatggc aaggtagagt tttccgcgaa agcgcccgat ggtacggcag
     1381 ctttcacttc ctggaagtgt cggcacttcg tcacgaacca tttcaactgg aagcactgcc
     1441 agcgactgcg cattcaattg aaataatcct gaggatgttc caaccagcac tacttccggg
     1501 gattcctggg acagcgcatg aattgcccgg ttccggcagg cgtcaactgg ctgccaggtg
     1561 gtctgatcca gactgcgcac aaacaaaccg cgattggtac cacaccagat tcggccatcc
     1621 gtagcctgca gaacaactcg gataaaattg ctttccgggt cgcttgaaat cgcctcaacc
     1681 gccgggctaa accgatcata gcgacagatg ccacggtcag ttccaaacca ggccacgcct
     1741 tcccgatcaa gaaataccgc attgatccga ttggaccgca agccgccagc agtattttca
     1801 aaagtgaggt gttcgatcaa ctcgcggcct ctcagatgaa aaaccccctg attttcggtt
     1861 ccaacccagg tttcgccagt gtcgccaaaa gccagtgccg tcactggccc ggtttggagc
     1921 ggcacccgtt gcgcctgttt caaatccggt ccttccagca acccgctgtc ccgcgccggt
     1981 gcctcgccac caaaccagaa cgccccggct gaatcctggc caaccgcgtt gacaaaatat
     2041 gcccgtggct gattggtgag cggctggagt tgcgtccctg acaccagcaa caccccgcgc
     2101 ccgccggtgc cgatgaccag cttgccgtcc acttcgccaa gcgacgtcaa cggcaagagg
     2161 gaatccggat cacgttcgcg gtgcaggagc ggataggtac tggccgaaaa tgagataact
     2221 tcaaactctg aatttcctcg cttgcgaatt tgcagcagcg tgccctgctc acaggccacc
     2281 gccacctgac cgtttttcag caaacgaatg gcccgaattg tcaaaccagc cgatctggcc
     2341 accagttcgc aacggtctcc ctgaaaccga tacagtccag attcactgcc aatccacagg
     2401 cttccatccg ggtccggttg caaacaggat actcgtgacg agggaacccc ggcagcggtt
     2461 attttttggg ttcgccgacc atcaaaccgg gccaggccat tgtctgtccc aaaccacagc
     2521 acaccttcct gatcctgggc aatggcccgt acctgatcgg aaggcagccc gtgaaagagc
     2581 gccacggctc cccactgatg ctggttgagt gacgagatag acgtgggttc agacggtgat
     2641 tgggccagca ccggccaacc agccatcaaa gtatttccaa ccagactcaa acagatcatc
     2701 agaaaaagtg tgcgcatcgg agaaccagga aatacgaata aagaatgaag tcagtatcac
     2761 ctgcgtcagt gggtggggtt ggaaagcaaa cccacccact gacgcaggtg atactgacaa
     2821 cccggcgtgg cattggaagg caaaaccagc ttgcccgtca atgtgacttg cctcagagtg
     2881 actccagatg gaaaaaagaa acgaagttct gtgtttttga ccgatttccg ataaaatgca
     2941 ttaccttata tcaatttgaa ttttcttctt tttcaccgga cacctgcatg gcacatctac
     3001 cgcgcccttc atccgctcct ttgattttga ttgtcgaaga cgataattca attcggttta
     3061 tgctcaagtc ctttctgaaa cgcgagggct atggtgtact cgaagcgcga cacggacagg
     3121 aagcactcga aatatttgaa gcgcaccggc ctgatatggt gctcttagac ttgatgatgc
     3181 cagtgcttga tggatttcag acctgttcaa gactgcgaca act
//
LOCUS       DAOQDO010000484         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23222_length_3215_cov_3.537342, whole genome shotgun sequence.
ACCESSION   DAOQDO010000484 DAOQDO010000000
VERSION     DAOQDO010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23222_length_3215_cov_3.537342"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKZ53_08985"
     CDS             <1..412
                     /locus_tag="PKZ53_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40611.1"
                     /translation="NQIRKMFDEIGHSVVKLRRVAIGHLTDEKLPIGMVRELTQKEVE
                     RFFQKARKTARQKATGKAPVETAPTTVEDLTARPQKLGKPKPQGTAKFKQKKRLTHTI
                     GKDPASKYGKRPTAKKRSIKRDGVKPQNSDTSQK"
     gene            622..1899
                     /gene="tyrS"
                     /locus_tag="PKZ53_08990"
     CDS             622..1899
                     /gene="tyrS"
                     /locus_tag="PKZ53_08990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNJ40612.1"
                     /translation="MTTHTILDELTWRGQVHDFTPGLVEALAKDSVTLYSGFDPTADS
                     LHVGSLVPIMGLVRFQRAGHKPIALAGGGTGLIGDPSGKSAERQLLTLEQVNANVEGI
                     KRILERYLDFSGAYAAQLVNNVDWLKEISILEFLREIGKHFTINAMMGKESVQRRIET
                     GISYTEFSYQLLQARDYLELFDRYGCSLQIGGSDQWGNITAGIDLIRRTRSQPAYGVT
                     FPLVTKADGTKFGKTEQGNVWLTEDRTSAYEFYQFWFNTNDQDVVRYLKCFTFLGQEE
                     IAALEHELQAHPDQRTAQKKLAAELTAFIHGPTALEQALQASRILFGEAITGISEATL
                     LTIFQDTPSSDISASLFTDGEASIVDLVVAAGLAPSKGEARRTIQGGGIYFNNVRVAA
                     PQERISRERFVSGRYGVLRKGVKNYHLVRIQAE"
     gene            2120..2605
                     /locus_tag="PKZ53_08995"
     CDS             2120..2605
                     /locus_tag="PKZ53_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNJ40613.1"
                     /translation="MRIQSKTKVRSTAELTLASPEILKLAQAELARHRTFRMRLSGNT
                     MRPTIHNGDWITIEPIKPGTLKAGDIVLLVTSSETAMIHRLIRLDFTKDSPYVLTRGD
                     STEYFDVPVPLSHVLGRISHIEHNGQKTDLTTFWQRVRTRLLGLKMRWQLRRTVFSAP
                     K"
     gene            2926..>3215
                     /locus_tag="PKZ53_09000"
     CDS             2926..>3215
                     /locus_tag="PKZ53_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNJ40614.1"
                     /translation="MVSPKVLARKLKIVGAPMDLGADRRGVDMGPSVVRIAGLSAKLQ
                     EMGYEVEDIGNVPVDLAETLTVGETDRRHKYLAQVVKSSEILAERVEWILEQ"
BASE COUNT          815 a          835 c          800 g          765 t
ORIGIN      
        1 taaccagatt cggaaaatgt ttgacgaaat cgggcattcc gtcgttaagc tccgccgggt
       61 cgcgattggg catttgacgg atgaaaaact ccccattggg atggttcgtg agttgactca
      121 aaaggaagtc gaacggtttt tccagaaagc cagaaaaacg gcgcgtcaaa aggccaccgg
      181 gaaagcacct gttgaaacgg ctccaaccac ggtcgaagac ctcaccgccc gtccccaaaa
      241 acttggaaaa cccaagccgc aaggaacagc caaattcaaa cagaaaaaac ggctcaccca
      301 caccattggg aaagaccctg ccagtaaata tggcaaacgc ccaactgcca aaaagcgaag
      361 catcaagcgt gatggggtga agccgcagaa tagtgatacc agtcagaagt agtcagtagt
      421 tcactaagct tacctggttg aatgacttaa ccgttttcca gacaagaatt tcattgcaaa
      481 ttggtatgaa ctggtgaagt agccagcgtt gccgttcgct cattgctgtc aatgacgaca
      541 ctctgcaggt ggggctattt cgcggcttca cgatttcgct attcgctctg attgttattt
      601 cgctcaaaaa acaaaaaatc tatgaccaca catacaatcc ttgatgaatt gacctggcgc
      661 ggtcaggtcc atgattttac ccctggcctg gtggaagcgc tcgccaaaga ctcagtgaca
      721 ctctattccg ggtttgaccc gaccgccgat agtctgcacg tcggcagcct ggtgccgatt
      781 atggggttgg ttcgatttca acgagccggg cataaaccaa ttgccctggc tggcggagga
      841 acgggtctga tcggtgaccc aagcgggaaa tccgccgaac gtcagcttct cacgctcgaa
      901 caggtcaatg ccaatgtaga aggtatcaag cggattctgg aacgctacct ggacttttca
      961 ggcgcttatg ccgctcaact ggtcaataat gtcgactggc tcaaggaaat ttcgattctg
     1021 gaatttttgc gtgaaatcgg taaacacttt accatcaatg ccatgatggg caaggaatcg
     1081 gttcagcggc gaattgaaac cggtatttcc tacaccgagt tcagttatca attgcttcag
     1141 gcacgtgact accttgaact ttttgatcgg tatggctgct cgctccagat tggcggcagc
     1201 gatcaatggg gcaatatcac cgccggaatt gacttaattc gccgaacgcg ctcgcagcca
     1261 gcctatggcg tcacgttccc actggtcacc aaagctgacg gcaccaagtt tggaaaaact
     1321 gagcagggga atgtctggct cactgaagat cggacgtcag cctatgagtt ttatcaattc
     1381 tggtttaata ccaatgacca ggatgtagtc cggtacctga aatgcttcac cttcctcggt
     1441 caggaagaaa tcgccgccct tgaacacgaa cttcaagcgc atcctgacca acgaaccgct
     1501 caaaagaagc tggcggctga attgacggcg ttcatccacg gtccgactgc cctggaacag
     1561 gccctgcagg catcccggat cttgtttggt gaagccatta caggtatcag cgaagccacc
     1621 ttgctcacga tctttcaaga cacaccgtcg tctgatattt cagcttcgct ctttaccgat
     1681 ggtgaagcca gcattgtgga tctggtcgtg gcggcgggtc tggcgccatc gaaaggcgaa
     1741 gcccggcgga cgatccaggg aggtgggatt tatttcaaca atgttcgcgt tgccgccccc
     1801 caggaacgca tttcgcgaga gcgttttgtg agtggacgct atggggtgct tcgcaaaggg
     1861 gtcaaaaatt atcacctggt gcggatccag gccgaataat tctcgctctc tttcgggttt
     1921 caggttgtgc tttcgcaaac ctgaaaccca aagccaggcg atcatcgttt gggagctgca
     1981 cctgtttttt cagcgagcaa aaaaccagcc acaaattcca gtgttgttca atccaggact
     2041 tgcacaattc aacctgctca ttatgattga gccgagcccc cgctcagtga tttttcagcg
     2101 ttacgggtga ccccacgcta tgcgcattca gagcaaaacc aaagtccgat ccaccgccga
     2161 actcactttg gcatcacctg aaatcctcaa actggctcaa gctgagctgg ctcgtcaccg
     2221 cacgtttcgg atgcgcctga gtggcaatac catgcggccc acaatccaca acggtgattg
     2281 gattacgatt gaaccgatta agcccggcac gctcaaagct ggtgatattg tgctcctggt
     2341 aaccagtagt gaaacagcca tgattcaccg attgatccgg ctcgatttca ccaaagactc
     2401 accttatgtt ttgacgcgtg gtgactcaac cgaatacttt gatgttccag ttccgctttc
     2461 acacgtcctt ggacgcatca gtcatattga gcataacggc cagaaaacag atttgacgac
     2521 attctggcaa cgggttcgca cacgattgtt agggttgaaa atgcgctggc agctccgccg
     2581 aacggttttt tcagctccta aataacccat cccaccagtt cttctctatt cgggctgatc
     2641 cgaaaacacc ccggtttcca caagtcgcat ctcgccgcct gcaacaaagt cgcttcgtgt
     2701 aactcattcg ttacaagctg gttttggttg ctttctctcg ctttgagccg caatatatcc
     2761 agagatcatc gttgcacaca tcgtgcacag tttgcctttt ttatccaaat caaaccatcc
     2821 acgagaaatc aggaagacag atcaggagga cgacctcaaa tcggggagtc gtttcgattt
     2881 gtttgtctgg tttatcgtcc cgaaattatt tgaggagaaa tttttatggt cagtcctaag
     2941 gtattagccc gaaaactgaa aattgtgggc gcgccgatgg acctcggagc agaccgacgc
     3001 ggggttgata tgggcccctc ggttgtgcgc attgcgggac tcagcgccaa actccaggaa
     3061 atggggtatg aagttgaaga tatcggaaat gttccagttg atctggcgga aacactcacc
     3121 gttggtgaaa ccgaccgccg tcacaaatat ctggctcagg ttgtcaaatc atctgaaatc
     3181 ctggccgagc gggttgagtg gattctggag caaga
//
LOCUS       DAOQDO010000485         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23247_length_3213_cov_3.144712, whole genome shotgun sequence.
ACCESSION   DAOQDO010000485 DAOQDO010000000
VERSION     DAOQDO010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23247_length_3213_cov_3.144712"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="PKZ53_09005"
     CDS             <1..1537
                     /locus_tag="PKZ53_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNJ40615.1"
                     /translation="VLVIAVLSLAGHIALVWAGNLRGFHPSILVAIRDMVLFVPIILS
                     VIWLVRVQKCRANLTLLTAAIFLMCFGQLAQYRLFSDPEYGARGEKRRDAREMKIQTI
                     LRTNIASGYDDVKKKYLFGSTEVPPAPDPVTSQIGFFDVATSSNTLVPVVSLVIMLGL
                     FVLMKRDDVLHLLQKNAFLIGLVTVVPFLIIVVFFSRAGKFLGETTPWEPVKLLFLVT
                     YGGLLADHHIHMSHTRWGLPPWRFLILLAVAALMPVIPFFFLSDFGQMLVFGGVYALL
                     YLIAVRRWTQLVYAVVLVGMLFPMFYFGIGIPNRINLRFHLWLDTWQAPPPETSWWKP
                     FSERIQREYEGRTITNQDAWFDQSAQIAQGLFGVSDGQLVGEGLGLGFPEVVPVSDSD
                     FVYVALSEELGLVGGFILLLGLCAFVMAGINVATAAPDMFSKLLAAGFTAFIGLQALV
                     NIGGVIRLLPMTGITLPFVSHGGWSLMTSFAMLGTLLALSHRNHREKTSPIIENPVAV
                     RVE"
     gene            1617..>3213
                     /locus_tag="PKZ53_09010"
     CDS             1617..>3213
                     /locus_tag="PKZ53_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40616.1"
                     /translation="MTRFFSAWHKSVGMGLLVLALTLWCQLSIQATTVVLMPDDDLIV
                     SSRVIVHGTVTQIESRFDPAGQSIYTYVTLHLQQVFKGNISDSTIVVRQLGGTVGGFQ
                     QVVYGSPEFAVGEEVLLFLNTADDGALRVAHLCLGKYSVVTDENGGRQVHRTLDGVEL
                     LEPTKHQVTGSASTSEMEWSRFLERVHSTLTNQADRVMTFDQLNDSRPLVTIPREYLG
                     SPSAGGFQPKFTVLGSGSRWFEADTNTPVIYRINTNNPPRNNGTPIPGNGVTEFTATL
                     AAWTNIANASLILQSGGTTTAVGRQNDGVNAISFNDPLNQIDDPVNCGGVLAIGGFTS
                     SNQTIVVSGRTFNRIIDADVAFNNNFDCFLGNPANLAEVMTHEVGHSIGLGHSSENVN
                     EANATLRDATMFFQAHGDGRGAVVRTDDIAGAAFIYPVAGGAATPTITSFNPTSGTVG
                     TAVTITGTNFTGASVVSFNNLNAAFTVNSATQISATVPNGATTGLIRVTTPGGTATSA
                     TNFTVNAGGGGDTQAPTVTVTSPN"
BASE COUNT          676 a          811 c          866 g          860 t
ORIGIN      
        1 cgtgcttgtg attgcggtat tgagtctggc cggccacatt gctctggtct gggcaggtaa
       61 cctccgtggg tttcacccaa gtattctggt tgccattcgc gatatggtgt tatttgtgcc
      121 gattattctg agcgtcatct ggctggttcg ggttcagaaa tgtcgggcga atctaacctt
      181 gttgacggcg gcaatttttc taatgtgttt tggtcaactg gcccagtatc ggttgttttc
      241 tgatccggaa tatggtgctc gtggggaaaa gcgacgtgat gcccgcgaaa tgaaaatcca
      301 aaccatcctg cggaccaaca tcgcttcggg atatgacgat gtgaagaaaa aatatttgtt
      361 tggcagcacc gaagtcccac cagcacccga tccggtcacg tcacagattg gtttctttga
      421 tgtggcgaca tcgagcaata ccctggtgcc agtcgtttca ctggtgatta tgcttggcct
      481 gtttgtgctc atgaagcggg atgacgtgct gcatctcctc caaaagaatg cgttcctgat
      541 tgggctggtc acggttgttc catttttgat catcgtggtg tttttcagcc gggctggaaa
      601 gtttttaggg gaaaccacgc cctgggaacc ggtcaaactt ctgtttctgg tcacctatgg
      661 tggcttgctg gccgaccatc atatccatat gagtcacacc cgatgggggc taccgccctg
      721 gaggtttctg attctgcttg ccgttgctgc tttgatgccg gtcattccat tctttttcct
      781 gagtgatttt gggcaaatgc tggtctttgg cggggtctat gccctgttgt atttgatcgc
      841 tgtccggcgt tggacacaac ttgtctatgc cgtggtgctg gttgggatgt tgtttccaat
      901 gttttatttt gggattggaa tccccaaccg aatcaatttg cggtttcatc tctggctcga
      961 tacgtggcag gcgccacctc ctgagacatc ctggtggaaa cctttttctg aacgaatcca
     1021 gcgcgaatat gaaggccgca cgattaccaa ccaggatgcc tggtttgatc agtccgccca
     1081 gattgcccag gggttatttg gcgtcagtga tgggcaactg gtgggtgaag ggctgggatt
     1141 gggctttcca gaagtcgttc cggtttcaga ttcagatttt gtctatgttg ctctcagtga
     1201 agagcttggg ttggttgggg gatttattct tttgctgggg ctctgtgcgt ttgtcatggc
     1261 cgggatcaat gtggccacgg cggcgccaga tatgtttagc aagctccttg ccgccgggtt
     1321 tacggctttt attgggctac aggccctggt caacatcgga ggggtcattc ggttacttcc
     1381 catgactggg atcaccctcc cctttgtcag tcacggcgga tggtcgttga tgaccagctt
     1441 tgccatgctt ggcacattac ttgctctttc tcatcgcaat catcgggaaa aaacatcacc
     1501 aattattgaa aatccagtgg cggttcgggt ggagtagagc gagtcaaagt ttcctgcctt
     1561 ccactcatcg ttcacgccac aaccactttt agaccaatcg tatttgagga gcctatatga
     1621 cacgattttt ctcagcctgg cataaatctg ttggaatggg gctgctagtt ctggcactta
     1681 cgctatggtg ccagttgtca attcaggcca ccacggtggt tctgatgccg gatgacgatt
     1741 tgattgtcag ttcacgggtc atcgtccacg ggacagtcac acagattgaa agccgttttg
     1801 atcctgcagg ccaatccatt tacacctacg ttacccttca ccttcaacag gttttcaaag
     1861 gcaatatcag tgattccacc attgtggtcc gacaattagg tggaaccgtt ggtggctttc
     1921 aacaggtggt ttacgggtca cctgaatttg ccgtgggcga ggaagtcctg ctctttctca
     1981 acacggctga tgatggcgcc ctgcgcgtgg cacatctgtg tttagggaaa tattcagttg
     2041 tcaccgacga gaatggtggc cggcaggttc accggacact ggatggagtt gaactccttg
     2101 aaccaaccaa acatcaggtc accggatctg ccagtaccag cgagatggaa tggagtcggt
     2161 ttctggaaag ggtccactca accctgacga accaggcaga tcgggtgatg acatttgatc
     2221 agctcaacga ttcacggcca ctggtgacga ttcctcgcga gtatctggga tctccgtccg
     2281 cgggtggttt tcagccgaaa tttacggtgt tgggcagtgg ctcgcgctgg tttgaagctg
     2341 acaccaacac ccctgtcatt tatcgaatca acaccaataa cccaccacgc aacaatggaa
     2401 caccaattcc aggaaacggt gtgaccgaat ttaccgcgac gctggcggcc tggaccaata
     2461 ttgccaatgc gtcattgatc cttcaaagcg gaggcacgac cacagcggtt ggtcgccaga
     2521 atgatggtgt caacgcaatt tcatttaatg acccgctcaa ccagattgac gatcccgtca
     2581 attgcggcgg agttttggcg attggtggtt ttacttcgag caaccagacg attgtggtca
     2641 gtggccggac ctttaatcgc atcattgatg ccgatgtggc ttttaacaac aactttgatt
     2701 gctttctggg aaatcccgcg aatctggccg aagtaatgac ccacgaagtc ggccacagca
     2761 ttggacttgg gcattcatcg gaaaacgtga acgaagccaa tgccaccctg cgcgatgcga
     2821 cgatgttttt ccaggcgcac ggcgatggtc gcggagcagt cgttcgaacc gatgatattg
     2881 ctggtgccgc ttttatctac ccggtggctg gcggtgccgc gacaccaacc attaccagtt
     2941 ttaatcccac cagcggcact gtgggcacgg cagttaccat caccggaacc aatttcacgg
     3001 gcgcttcggt ggtgagcttc aacaatctca atgctgcctt tacagtgaat tcagccaccc
     3061 aaatcagtgc taccgtaccc aacggcgcca ccacgggttt gatccgagtc acaacgcctg
     3121 gtggaaccgc caccagtgcc acgaatttta ccgtgaatgc cggcggtggc ggcgataccc
     3181 aggcgccaac tgtaacggta acgtcaccca acg
//
LOCUS       DAOQDO010000486         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23260_length_3212_cov_3.196389, whole genome shotgun sequence.
ACCESSION   DAOQDO010000486 DAOQDO010000000
VERSION     DAOQDO010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23260_length_3212_cov_3.196389"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..840)
                     /locus_tag="PKZ53_09015"
     CDS             complement(187..840)
                     /locus_tag="PKZ53_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ40617.1"
                     /translation="MTTDEYFMTLQTAELTEHHFLGTSELADAAAQLVLQVVPAQNRG
                     NVSDLPDERTVRYYLAQSLLSEPLGRKGSASIFGYHHLLELLVIKRLQSQNMRIKQIR
                     EQLTGKSTAELEALLEIPGSTAPLTEFEPPDTSATAFLESLLAEKSVDPGATQRQTGT
                     LLASASQPAQSAWTRIELEPGLELHIQTGWQFPAEATARHRLARRIQDALLEAGRRK"
     gene            complement(1169..>3212)
                     /locus_tag="PKZ53_09020"
     CDS             complement(1169..>3212)
                     /locus_tag="PKZ53_09020"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HNJ40618.1"
                     /translation="TGQPVGEIQVLTGGKRTASVYADTDVVVLKIPRATFELAISRVP
                     HLISRVTQIIQIRLQRELLLTVLPPLVGPLDDEALRMAEEYAEWVYLQQGGCLFSQGD
                     VGGAVYLVMSGHLYAILSDHSGNQRVLNDIGRGEIIGEIAFFTHEARTASVFASRDSI
                     LIRFNHASFTRLSERYPNITQFIIQMLIKRLRTRELHQGNKGSIRYGGFGQQSTTRVR
                     WSSVTRKISNIAIIPLSDRVPVELFAQKLETALRTFGKVAVLTAERVDAQLGNPGISQ
                     SSVEDPLSIRLTAWLDRQELLYQFVVYVAEPVASAWTQRCVRQADRILLAGMAHESPA
                     LRETEDRFLAGMNSITSPPQSLVLIHPNRDRLPSGTRQWIDLRPVEIHFHIVMDRQED
                     FERLARVMTGHAVGLVLGGGGARGLAHLGVYRALQEIGIPIDIIGGTSMGAIAGAQIA
                     LQWNWEQIRSRNKVAWVDMQPAADYTIPMFAFLKSHRADKAMEHSFETHQIEDTWINF
                     FCVSSNLTTAEIMIHREGPLAHALRASSSLPGVFVPVIDGKNILVDGAVFNNLPGNLV
                     RDFGSGFNIVINVSPEEDLIPKHERFPSAWRALKDRFLHGEKNPGVPTIFDTIIRSLL
                     LSSTNVANSVQRDADLYLAPPVNEFGMLEFTAFEQIMEAGYRYSVDTLKYFKLPWT"
BASE COUNT          761 a          807 c          838 g          806 t
ORIGIN      
        1 ggttccgggt tccgggttcc gggttccggg ttccgggttc cgggttttcg aagagaagtt
       61 tcgtcctttt tgccgctggt tatttttcct ggccttatac cgttttgcaa tgaaaccctc
      121 gtgtcacagg aaggctaaat tgttgaaaaa attgtatttc cccgataccc tgaaccctaa
      181 ctggtattac tttcggcggc cagcttccaa cagagcatcc tgaatgcgtc gggccagtcg
      241 gtgtcgggcg gtggcttcgg ctggaaattg ccagccggtt tgaatatgta attccagtcc
      301 tggttcaagc tcaatccggg tccaggctga ttgcgctggc tggctggcag aggccagtaa
      361 ggtaccggtt tgccgttgcg ttgcgcctgg gtcaactgat ttttcagcca gtaatgattc
      421 cagaaatgcg gtggctgagg tgtcaggcgg ttcaaattcg gtgagtgggg ccgttgatcc
      481 tggaatttca aggagtgctt ccagttcggc ggttgatttc ccggtcagtt gctcacgaat
      541 ctgtttgatt cgcatatttt gggactgcaa ccgtttgatg accagcaact caagcaaatg
      601 gtgataccca aaaatcgaag ctgatccttt gcgcccgagc ggctctgaca gcagcgactg
      661 cgccagataa tatcggacgg ttcgttcatc aggtaaatca ctgacattgc cccgattttg
      721 agctggtacc acctgcagaa ccagttgggc cgccgcatca gccagttcag aagtgccaag
      781 aaagtggtgt tcagttaatt cagccgtctg caatgtcata aaatactcgt cggtggtcat
      841 cagtcatcag gttgggtttt tgcctggttt tctctcatct aaggttgaca caagatacgt
      901 tagaatattt ttaatgtcaa cagtattttt cctgtaaaac caccttatat tctaattttg
      961 atacaaatgc ttattttact tatatttaag tatagaagaa aaacgaaaaa tctatgtact
     1021 gttgacagtt ttctgatgct gtacatctgg tgaagacagt aggatgcctt ttacccaaac
     1081 attcgttctc tggctttggg ttggctatga gtcaggtgac tggtagacag taacctcgtt
     1141 cgagatataa cgcgatgtct tgccagtttc aggtccaggg caatttaaaa tacttgaggg
     1201 tatcaacgct gtagcgatag ccggcctcca tgatttgttc aaaggcagta aattcaagca
     1261 tcccaaattc attgactgga ggggccaggt acaaatcggc atcccgctgt acggaattgg
     1321 cgacgttggt gctactgagc agcaacgacc ggataatggt atcaaaaatg gttgggacac
     1381 caggattctt ttcaccgtgc agaaagcgat cttttaacgc cctccaggcc gaaggaaacc
     1441 gttcgtgttt ggggatcaga tcttcctctg gactcacatt gatcacaatg ttaaacccgc
     1501 ttccaaaatc gcggacaaga ttcccaggca agttattaaa gaccgcaccg tcaaccaaaa
     1561 tgtttttccc atcaatcacc gggacaaaga ccccagggag cgaactgctg gcccgcaagg
     1621 catgagccag cggcccctcg cggtgaatca taatttcagc cgtcgtcagg ttgctggaaa
     1681 cacaaaagaa gttgatccag gtgtcttcaa tttgatgggt ttcaaaggaa tgttccatgg
     1741 ctttatcggc acgatggctt ttcaaaaacg caaacattgg aatggtgtaa tctgccgcgg
     1801 gctgcatatc aacccaggcg actttgttcc ggcttctgat ctgttcccag ttccactgaa
     1861 gtgcaatttg ggccccggca atggcgccca tgctggtgcc tccgatgatg tcaattggaa
     1921 tgccaatttc ctgcaaggct cgatacacgc ccagatgcgc caacccacga gcgccgccgc
     1981 cacctaaaac cagacccaca gcatggccgg tcatcacacg ggcgagtcgt tcaaagtctt
     2041 cctgccggtc catgacgatg tgaaaatgga tttccaccgg gcggaggtca atccactgcc
     2101 gggtgccgct ggggagcctg tcccgattgg gatggatcaa caccagcgat tgaggtggcg
     2161 acgtgattga attcatcccg gcgagaaagc ggtcttcggt ctctctcaag gcaggtgatt
     2221 cgtgggccat cccagccagc aaaatccgat ctgcctgccg gacacagcgt tgtgtccagg
     2281 ctgaggccac cggttcggcg acatacacaa caaattgata gagcagttcc tgccggtcaa
     2341 gccaggcggt gaggcgaatg ctcagtggat cttcaacact ggattgagaa atccccggat
     2401 ttcccaactg tgcatcaacg cgttcggcgg tcaacaccgc aactttgcca aaggtgcgca
     2461 gggcggtttc cagtttttgc gcaaagagtt caaccgggac acgatcactg agggggataa
     2521 tcgcaatatt tgaaattttg cgggtgactg atgaccagcg gacacgtgtc gttgactgct
     2581 ggccgaatcc tccgtagcgg atgctgcctt tgttcccctg atgcagttcg cgagttcgca
     2641 accgcttgat cagcatttga ataataaact gggtgatgtt tgggtaccgt tcgcttaacc
     2701 gggtgaagct ggcgtgatta aaccggatca gaatactgtc acgtgaggca aagacactcg
     2761 cggttcgggc ttcgtgcgta aaaaaggcga tttccccaat aatttctcca cggccaatat
     2821 cgttgaggac acgctgattt ccgctgtgat cactcaaaat ggcatacaag tggccactca
     2881 tgaccagata cacggcgcct ccgacatctc cctgggagaa aaggcatccg ccttgttgca
     2941 ggtacaccca ctctgcgtat tcctcggcca tgcggagggc ttcatcatcc agtggaccaa
     3001 ccagcggagg cagcaccgta agcagtaact cccgctggag ccgaatttga ataatctgag
     3061 tgacccggct gatcaaatgg ggaacgcggc tgatggcgag ttcaaatgtg gcgcgaggaa
     3121 ttttcaagac cacaacgtct gtatcagcat agacactcgc cgttcgcttg ccaccggtta
     3181 atacctggat ttctccaacc ggttgcccgg tt
//
LOCUS       DAOQDO010000487         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23285_length_3210_cov_2.626624, whole genome shotgun sequence.
ACCESSION   DAOQDO010000487 DAOQDO010000000
VERSION     DAOQDO010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23285_length_3210_cov_2.626624"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2876)
                     /locus_tag="PKZ53_09025"
     CDS             complement(93..2876)
                     /locus_tag="PKZ53_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ40619.1"
                     /translation="MIHQGLLAVVKEVAAEIHPGELSRPISLATRLDYDLGFDSLSRV
                     ELLSRIEQVFSVKLPETALMQVETPGDLLKLLEQAGSQPRETSPILPLQPVAELAESP
                     STEHLSTLTEILTHHAQVHGDRPYLRFCETGQTITYTDLYQDSLKIATGLRRRGLLPQ
                     QAVGLMLTTSREFFISFFGCLFAGCVPVPLYPPSRISQLADHLQRQVRILDNAEAVLL
                     ITFDQAKPFTALLQAKVGSLQEIVAAEDLYYAGEPLDPVSVSPQDTAFLQYTSGSTGT
                     PKGVVLSHANLLANVRAMGTAFKVTSSDVFISWLPLYHDMGLIGACLGSLHYACLLVV
                     MPPQNFLARPERWLRAIHDYRGTIAAGPNFGFELCLRKIKDEDLQGLDLSSWRMALNG
                     AEPVSPRTMDQFCDKFAAYGFHREAMSPVYGLAECSVGLTGPPPGRGPNVEWIDRHAF
                     TEQGKAVPVQPNDPHAMPVVGCGFPFPDHQVRVVDKLGYEVPDRVQGKIQFRGPSATV
                     GYFRNPEATAALFRGEWLETGDLGYIAGGEIFIAGRSKDIIIRAGRNLYPQELEDIVG
                     NLPGVRTGCVAVFACSDTHSGTERLVIVAETRETSPEVLEQLRTQINQATLQLLDSAA
                     DEIVLAPPHSVLKTSSGKIRRSGTRDAYLNGDLFRPRSPFRDRLNALKLKTAGTFQSM
                     IHRTGLFLFGAWCWLVFVSAVLISCVLLVVIPTLKLRRSYGRWLARLVTIACGMPVAV
                     DGAEHLPTDQACVYVANHASYIDAIVIMAILPPNTCFTAKKELAENWLIRFALDRLGT
                     QYLDRFDFQESLNNIARLEELAKAHQSLGFFAEGTFTREPGLRAFHLGAFKTAATARL
                     PVVPVALQGTRSILRDEQWLPHRGDLHVTIFPPIVPRGTDWSSVITLRDTARAAILEK
                     CGEPDLANIKWPK"
     gene            3100..>3210
                     /locus_tag="PKZ53_09030"
     CDS             3100..>3210
                     /locus_tag="PKZ53_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNJ40620.1"
                     /translation="MNLINVFDFEKLAQEKLFQMAFDYYASGAHDEITLRE"
BASE COUNT          779 a          840 c          860 g          731 t
ORIGIN      
        1 cctgagcgat tgaatgttgg aagtgtttgg tttttttcgt gtatttcgtg tgttttgtgg
       61 ttcaaaatgt ctgggaattt tcggtaaggt acttatttag gccatttaat gtttgccaga
      121 tccggctctc cgcatttttc cagaatggcg gcccgagccg tatctcggag ggtaatcacc
      181 gacgaccagt cagttcctct gggcacaatt ggaggaaaaa ttgtcacatg gaggtcgcca
      241 cgatgcggca accattgttc atcccgcaaa attgaccggg tgccctgtaa ggccaccgga
      301 acaactggca acctggcagt cgccgcagtt ttaaaggccc caaggtgaaa ggcgcgcagg
      361 ccgggttcac gggtaaacgt accttcggca aaaaaaccaa gtgattgatg agctttggcc
      421 aattcctcca atcgggcaat gttgttgagg ctttcctgga aatcaaaccg gtcaaggtat
      481 tgtgtcccga gccggtcgag ggcaaaccga atcaaccagt tctcggccag ttctttcttg
      541 gccgtaaagc acgtgtttgg cggcaaaatg gccatgatca caatcgcatc aatgtagctg
      601 gcgtgattgg caacatagac acaggcttga tctgtcggca ggtgttcggc gccatcaacg
      661 gcaactggca tcccacaggc aatcgtgacc agccgggcca gccagcggcc atagctgcgt
      721 ctgagcttca gcgtcggaat caccaccagc aacacacacg aaatcagaac cgctgacaca
      781 aaaaccagcc agcaccaggc cccaaacaag aacaaaccgg ttcggtgaat catagactga
      841 aacgtgccgg cggttttcaa tttgagcgca ttgaggcgat cccggaatgg gctgcgtggg
      901 cgaaacaaat ctccgttcag gtaggcatct cgggttccag accgccggat tttgccgctc
      961 gatgttttta aaacggaatg cggcggggcc aaaacaattt catcagcggc tgaatccagc
     1021 agttgcaacg tggcttgatt gatttgggta cggagttgtt ccagcacctc ggggctggtt
     1081 tccctggttt cagctacaat caccagccgt tcggtgccgg aatgtgtatc cgaacaggca
     1141 aacacggcga cacagccagt tcggactccg ggtaagttcc ccacgatgtc ttcaagctcc
     1201 tggggataga gatttcgccc ggcccggata atgatgtctt tgctgcggcc agcaataaaa
     1261 atttcaccgc cggcaatgta gccaagatca ccggtttcaa gccattcgcc ccgaaagaga
     1321 gccgccgtgg cctccggatt ccgaaaataa ccaacggtgg ctgacggccc ccgaaactgg
     1381 attttgcctt gtacccggtc tggaacttca taaccgagtt tatccaccac tctcacctga
     1441 tggtctggga agggaaagcc acacccgacc accggcatgg cgtgtgggtc attgggttga
     1501 actggaacgg cctttccctg ttctgtaaat gcgtggcggt caatccattc aacattcggc
     1561 ccccggccag ggggaggccc ggtaagtccg acggaacact cagccagccc atacaccggt
     1621 gacatggctt cgcgatgaaa tccataggca gcgaatttat cgcagaattg atccatcgtc
     1681 cgtgggctga caggttcggc tccattgagc gccattcgcc agctactgag gtcaagcccc
     1741 tgcaagtctt catccttgat tttgcgcaga cacaactcaa acccaaagtt tggacccgct
     1801 gcgatggtgc cccggtagtc gtggatcgct ctcaaccagc gttctgggcg ggccagaaag
     1861 ttttgcggcg gcatcacgac caggaggcag gcataatgga ggcttcccag acaggcgcca
     1921 attaagccca tatcgtggta gagcggaagc cagctaataa aaacatctga tgaggtgacc
     1981 ttaaacgccg tgcccatcgc gcgaacgtta gccagcaggt tggcatggct taaaaccacc
     2041 cctttgggtg tgcctgtgct cccggaggtg tattgcaaaa aggcggtatc ctgtggggaa
     2101 actgaaaccg ggtccaaggg ttcgccggcg tagtaaagat cttcagcggc aacaatttcc
     2161 tgcaacgatc caactttggc ctgtaaaagc gcggtaaatg gtttggcctg atcaaaagta
     2221 atcagcaaaa cagcctcggc attgtcaaga atgcgaacct gacgttgcag gtgatcagcg
     2281 agttgggaaa tccggctcgg tggatagagc ggaacgggaa cacatccagc aaacagacat
     2341 ccaaaaaagc taataaaaaa ctcacggctg gtcgtcagca tcagcccaac tgcctgctgg
     2401 ggcaggagcc ctcgccgtcg caagcccgtg gcaattttta aggaatcctg gtacaggtcg
     2461 gtataggtaa tggtttgtcc cgtttcacaa aaccggaggt acgggcgatc tccgtgaacc
     2521 tgtgcatggt gggtcaaaat ttcagtcagt gtggaaagat gctcggttga cggactctca
     2581 gccagctcag cgactggctg cagggggaga atcgggcttg tttcccgtgg ctgacttcca
     2641 gcttgttcaa gtaatttcag gagatcccca ggggtttcca cctgcatcag tgcggtttcc
     2701 ggcaacttga cggaaaaaac ctgttcaatc cgagacagaa gctcgacccg gctcaggctg
     2761 tcaaacccaa ggtcataatc cagccgcgtc gcaagtgata tcggtcggct gagttctccg
     2821 ggatgaatct cagccgccac ttctttgaca accgccagta atccctgatg gatcataggc
     2881 ttatcttgac tcgatgtagt ttgcatggtg gctgggtgct ccttggtgag gtctttggga
     2941 aaaatattga gtgtttatat ctattttctg gagatgcgga aaggatgagt caccataaaa
     3001 gcaccttttg cacaattcaa tgattcgcgc tacagttttc aaaccatcac tgtgcccctc
     3061 cttgatctca tccccatcct tcaggtccga aaatatccta tgaatctgat caacgtgttt
     3121 gattttgaga agcttgccca ggaaaaactc tttcaaatgg catttgatta ttatgccagc
     3181 ggcgcccacg atgaaattac cttgcgggaa
//
LOCUS       DAOQDO010000488         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23393_length_3202_cov_2.947887, whole genome shotgun sequence.
ACCESSION   DAOQDO010000488 DAOQDO010000000
VERSION     DAOQDO010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23393_length_3202_cov_2.947887"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKZ53_09035"
     CDS             <1..831
                     /locus_tag="PKZ53_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ40621.1"
                     /translation="RGLDRQAFALLEDGKEQEIFSFAKESDLPLTFALLIDVSGSQEL
                     SLPAEKEAALKFFDKVMRPDKDIAAVVTFRRDVELVQTLTGNKRALLGALNSIRFHPG
                     SYVGGSTPPVNTDPSYNGTSIYDAVFVTSDELLSREAGRRIVVLLTDGQDTTSQYSRD
                     KAVLCALRSEVMVYVIGIPGKGYYGSGRVFSEPVNKGAMRELAEQTGGRAFFPEKESD
                     FYSAFQQIEEDIRQQFSVSYSPSNSNRDGSFREIKIGIKGRPDTKDLKVLSRKGYYAK
                     "
     gene            1006..3021
                     /locus_tag="PKZ53_09040"
     CDS             1006..3021
                     /locus_tag="PKZ53_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40622.1"
                     /translation="MAESLLASSSTAPLSPPDLEAGQGVLNQQALSYFKLLTVLRKQL
                     TTTAATSQVGVVTNIARWLDAAPVLTLIARTPHWYCEWQIKEALYSNPRTPRRIREEI
                     GRTISIFELMRELDRHGLKDQEKTEIREDIKSLFTTLSGIDKSIVKARAYRLSSSNRQ
                     AAAGRPPTLETPLLPDLGALPEYSLADLLAITKELQGETGSLDESLSKPPEVVDLTSL
                     SVDEQLVQAQTTGDSFILLQALGSDREDLLSAVLTNPNLSEAHVLVAATENPNPAFFR
                     AMTHQERWYQQPEVRSALLKNPHLPRDRKLGLTLTEQLPTLFEAIDEGGDRAQAAAKT
                     LGNVIDHLGAEDLIYGLETAKVLYPHLVHLIFAAPVFTGQAELPLSVADEADIYEIDH
                     DFEAQLHSASQPVQIVSTADAINEPVPASRLSSAIELRCQEAATVNEIGQIALLLTDP
                     DDQVFASLLHNPALPEDMIVGYARTITADRAQAIYRDSRWAGRQRIQEALLDNPKTPH
                     GIALELLNFVSNPQSLLKVVKNPKIATIEVKQKALQKLIEIYRALDSSGRISLIKATN
                     GEVLHELWEEAFHDEETITTLLKEQYLEDGIVLKVVRSKSAPRSALGAIGANPAWINN
                     YQIRLELVMNPKTPRDVVARLISRLNPTDRTKIKNNRNLPEYVRAMV"
BASE COUNT          809 a          825 c          813 g          755 t
ORIGIN      
        1 cggggcctgg atcgccaggc attcgcatta ttggaagatg gcaaggaaca ggaaatcttt
       61 tcatttgcca aagagtcgga tttgccgctg acctttgcgc tgttgattga tgtgagtggc
      121 agtcaggaat tgagtcttcc ggctgaaaag gaagcggcct tgaaattttt tgacaaggtc
      181 atgcgaccag ataaggacat tgctgccgtg gtgaccttcc ggcgcgatgt cgaactggtt
      241 caaacgctaa ccggcaataa acgagcgttg cttggggcac tcaattcaat ccggtttcat
      301 ccgggttcat atgtcggtgg ctcaaccccc ccggttaata ccgatccatc gtataatgga
      361 acaagcattt acgacgccgt gtttgtgact tcggacgagt tgctttcccg cgaagctggg
      421 cggcggattg tggttctgct cactgatggt caggacacaa caagccagta ttcacgcgat
      481 aaggccgtgt tatgtgcctt acggtcagaa gtgatggtgt atgtgattgg cattcctgga
      541 aaagggtatt acggcagtgg ccgcgtattt tcagagccag tgaacaaagg ggccatgcgc
      601 gaattggcag aacaaaccgg tggtcgagcc tttttcccgg aaaaagaatc tgatttttat
      661 tcagcctttc agcaaattga agaagatatc cggcaacagt ttagtgtttc ttactcacca
      721 tcaaattcca accgagatgg ttcttttcgc gagatcaaaa ttggaattaa aggtcgtcca
      781 gatacaaagg acctgaaagt tttaagccgg aaaggctatt acgcaaagta aggaaacaaa
      841 cttttgaggc gcaggaacta aaggcacttg acggaatggt ttcttgggtg aaaattggtg
      901 gcagcacaat ccttcacact gccctgacca aagatccgat tccaaccagg tgaacttccc
      961 aggcaaaatg ctggttcctg cgccttttgc tttgcattga cgctcatggc tgaaagtctg
     1021 cttgcttcgt cctcaactgc gccgctgtca cccccggacc tggaggcagg ccagggtgtg
     1081 cttaaccagc aggcgctttc ctacttcaag ctcctgactg tattgcgcaa gcaactgacc
     1141 acaacggcag ccacgtccca ggttggggtg gtcaccaaca ttgcccgctg gttggatgcc
     1201 gcacccgtcc tgactctgat tgcccgcacc ccgcactggt actgtgaatg gcagattaaa
     1261 gaagccctgt attccaatcc acgcaccccg cgtcggattc gagaggaaat tggccgcacg
     1321 atctcaattt ttgagttaat gcgcgagctg gatcggcacg ggttaaagga tcaggaaaag
     1381 actgaaatcc gcgaagatat caagtcgctc tttaccacgc tgagcggaat tgacaagtca
     1441 attgtcaaag ctcgtgctta ccggttgtcg tcatcaaacc gccaggcagc ggctgggcgc
     1501 cctccaacgc ttgaaacacc attattgcct gacctcggag cgttacctga atattcactg
     1561 gccgatctgc tggccatcac caaagaatta cagggtgaaa ccggttcact cgatgaatca
     1621 ttgagcaaac cacccgaagt ggtggatctg acctcgcttt cagttgatga acaactggtt
     1681 caggcccaga caactggtga ctcattcatt ttgttacagg cacttggttc agaccgggaa
     1741 gacctcctca gtgccgtcct caccaatccg aatctttcag aagctcatgt gctcgtggcc
     1801 gcaactgaga accccaaccc ggctttcttc cgggccatga cccatcagga gcgctggtat
     1861 caacaacctg aagtccgaag tgcactttta aaaaatccgc acctcccgcg agatcgaaaa
     1921 ctcgggctga cattgaccga gcaattacct accttgtttg aagcgattga cgaaggtggt
     1981 gaccgggcac aggcggcagc caaaacgctc ggaaacgtga ttgaccacct tggggcagaa
     2041 gatctgatct atggcctgga aaccgcgaaa gttctctatc ctcatctggt ccatctgatt
     2101 tttgcggcac cggtctttac aggccaggct gaattgccat tgagcgtggc tgatgaggct
     2161 gatatttatg aaattgacca cgatttcgag gcccaactgc actcagcttc gcagccagtt
     2221 caaattgttt caacggcaga tgcgatcaat gagccggttc ctgcttcacg gctgtcgtca
     2281 gccattgagt tgcgatgtca ggaggcagcc accgtcaatg agattggtca aatcgcactt
     2341 ttgctgacgg atcctgatga ccaggtattt gcttcattgc tgcacaaccc ggcgctgcca
     2401 gaagatatga ttgttggcta tgcccggacc atcacggctg atcgagccca ggcaatttat
     2461 cgtgactctc gatgggctgg tcggcagcgt attcaggaag ccttgctcga caatccgaag
     2521 acgcctcacg gcattgcgct tgaacttctt aattttgttt caaaccccca aagcctgctc
     2581 aaggtggtga aaaatcccaa gattgccacc atcgaagtga aacaaaaagc cctccagaag
     2641 ctcatcgaaa tttatcgtgc gcttgattca tctggacgaa tcagcttaat caaagccacc
     2701 aatggcgaag tcctgcacga gttgtgggaa gaggccttcc acgacgaaga gacgattacc
     2761 acactactga aagaacagta tctggaagac gggattgtgt taaaagttgt ccgctccaag
     2821 tctgctccac gaagcgcact gggtgctatc ggtgccaatc cagcctggat caacaattat
     2881 caaattcgac ttgagctggt gatgaatccc aaaaccccac gtgatgtggt ggcccgcttg
     2941 atttcacgac ttaacccaac agatcgtacc aaaatcaaaa acaaccggaa cttgccggaa
     3001 tatgtccggg caatggttta gggcttgagg ctgaaaaaac tggggctcag ggcttggggc
     3061 tgaagaagtc gggctgaagg catcgggctg aagaaaatgg gctgaagaat tggttttacc
     3121 ccatgtcttc aaccccaagc ccgatgtctt caaccccaag cccgatgtct tcaaccctaa
     3181 gcccgatgtc ttcagcccaa tg
//
LOCUS       DAOQDO010000489         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23408_length_3200_cov_3.002862, whole genome shotgun sequence.
ACCESSION   DAOQDO010000489 DAOQDO010000000
VERSION     DAOQDO010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23408_length_3200_cov_3.002862"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKZ53_09045"
     CDS             <1..984
                     /locus_tag="PKZ53_09045"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ40623.1"
                     /translation="EYAGMGATFTAAGVDHTTLYLAQIGDSRAYLIRNGRIAQMTKDQ
                     SLVSQLVEAGHITEEEAESHHYKNVILQALGATSSVIVVVDCVQLQRDDILLLCSDGL
                     SGKVRGPEMLQIFNACNGDLTEGCRKLIQTANERGGEDNITVMLMHFSGEGLDAPTDS
                     NNFESRVIQRDPRLPDEIDESLLIYEKDTLDAQNKATGPLTVPTTTAVLSAAVVAELY
                     EVAAASGQPSVAPPPPAPSPPMPALPTPVRQNQNNSKLIAASLFMLALIVVAFLLYRK
                     NTAPTAAESPPAQPKPTPTAPAQPSPAPPPASQPSTPPSTPPASEPAKENK"
     gene            complement(1132..2394)
                     /locus_tag="PKZ53_09050"
     CDS             complement(1132..2394)
                     /locus_tag="PKZ53_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40624.1"
                     /translation="MASSPFSSRTNFREIIVLALDALNAHRLRSFLTILGVIIGVSTV
                     IGMVSIIQGLNRAFANQIESLGSNTIFISKFDPSFRRTRTSEERQRKDLTVEDGLAIG
                     REAPSILSVSPERRRNNISLRYEARVMDTSQLAGVLPTYEITRSNYTAGGRFFSDMDV
                     VHRTETCVLGMDVVDALFPNDDPIGKVIRMDGHPLKVIGVLERMGNFFGQTRDNVILV
                     PLTTFEKYYSDFSEQGGSFFFVIARPRNRRMVSRAVEQITDVLRRRRNVPFGEKDNFG
                     ISTQDSLLDIYNQLTGATALVLTAVSFISLGIGGIGVMNIMLVSVVERTREIGVRKAL
                     GARRVDIRQQFLLEAVTLTGIGGIIGVIVGQTISLLINRFSPLPSTVPWWAMAAGLTV
                     SVVVGLFFGLYPAIKASELSPIEALRYE"
     gene            2781..>3200
                     /locus_tag="PKZ53_09055"
     CDS             2781..>3200
                     /locus_tag="PKZ53_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40625.1"
                     /translation="MESLAPFLVWGLTGLLIVLVIGVVILLGIRSYTAWRDRIRLDMR
                     DTTRSKLLTAIRELADPNNNGLTWVVKPLKPKSQDETAVLKQVMTELSRVLSDEDRWR
                     LAELYEGTGMQAEDMQILRDTKKPWYERARTAYRLGQM"
BASE COUNT          847 a          839 c          790 g          724 t
ORIGIN      
        1 gaatatgccg ggatgggcgc cacctttacg gcggctggtg ttgaccacac aacgctgtat
       61 ctggctcaaa tcggagattc acgggcctat ctgattcgca atggccggat tgcccagatg
      121 acgaaagatc aatcgctggt cagtcaactg gttgaagccg ggcatattac ggaagaggag
      181 gctgaaagcc accactacaa gaatgtgatt ctccaggcgc tcggggcaac ttcctcagtg
      241 attgtggtcg tggattgtgt gcaattacag cgggatgaca ttttgttgct ctgtagcgat
      301 ggcttatccg gtaaggtgcg cgggcctgaa atgcttcaga ttttcaatgc ctgcaacgga
      361 gacctcaccg aaggctgccg caagttgatt caaacagcca atgagcgggg cggggaagac
      421 aatatcaccg tcatgctgat gcatttttca ggcgagggcc tggatgcccc gaccgatagc
      481 aataactttg aatcacgagt catccagcgg gatccacgcc tcccggatga aattgatgaa
      541 agcctgctga tttatgaaaa agatacgctt gacgcgcaaa acaaggccac aggcccattg
      601 accgttccaa ccacgaccgc cgttttatca gcggcagtcg tggctgaact ctatgaagtt
      661 gcagcggcaa gcggacagcc gtcagttgca ccaccaccac cggccccttc tccacccatg
      721 ccggcgcttc cgacacctgt ccgccagaac caaaacaatt cgaaacttat tgctgccagt
      781 ctgttcatgc ttgccttgat cgtggtggct tttttgctgt atcggaaaaa tacggcgcca
      841 actgcggctg aatcacctcc tgcccagcca aaaccaactc caactgcccc tgctcaacct
      901 tcccctgccc cacctccagc cagccagcct tcgaccccac cttcaacccc acctgcaagt
      961 gaaccggcga aagagaacaa gtaatcgttc ctgtcgcctt tctttttatc aagggaaatt
     1021 tgtttgtcca aaaatttcaa ttgtatcagg aaacgtgtct tcactgacca ggttgtcgcc
     1081 cagtatctgg tgactggaag ccaatgtgtt caaagcacga aaaaccagca gctattcata
     1141 tcgaagcgcc tcaatcggag ataattcaga tgctttaatt gccgggtaga gtccaaaaaa
     1201 taatccgaca accactgaaa cagtgagtcc agccgccatc gcccaccagg gaaccgttga
     1261 gggcaggggg gaaaaccgat tgatgagtaa actgatggtc tgtccgacta tcacaccaat
     1321 gattccccca attccggtca gggtgacagc ttccagcaaa aattgttgtc gaatatcaac
     1381 ccggcgagcg ccaagggctt tacggacacc gatttcccgg gtgcgttcaa ccaccgatac
     1441 cagcataata ttcattaccc caatgcctcc gattccaagt gaaataaagg aaacggcggt
     1501 taataccagg gccgtcgcgc cggtgagctg gttatagatg tcgagcaagc tatcctgagt
     1561 ggaaattcca aagttgtctt tttccccaaa gggaacattt cgccgccggc gcaacacatc
     1621 ggtaatttgt tcgacggccc gcgaaaccat gcgccggttc cgtggacgcg caataacaaa
     1681 gaaaaacgag ccaccctgtt cggaaaagtc tgagtagtat ttttcaaagg tcgtcaatgg
     1741 aaccaaaatc acattatcac gggtttgccc aaagaaattt cccatccgtt cgagtacgcc
     1801 aatcaccttg agcgggtgac catccatgcg gataaccttt ccaatcgggt catcatttgg
     1861 gaacagcgca tccaccacat ccattcctaa gacacaggtt tcagtgcgat gaacaacatc
     1921 catgtcggag aaaaaccgcc cgcccgccgt gtagttactt cgggtaatct cataggtcgg
     1981 taaaaccccg gctaattgag aagtatccat gactcgggct tcatatcgga gtgaaatatt
     2041 gttgcgtcgg cgctcaggtg atactgacag aatggatggc gcttcacgac caatcgccag
     2101 cccatcttcg acggtcagat ctttgcgctg gcgttcttca ctggttcgtg ttcggcggaa
     2161 gctcgggtca aatttggaaa taaaaatggt atttgacccc agggattcaa tttggttggc
     2221 aaacgcccgg ttgagtccct gaatgatcga gaccatcccg atgaccgttg aaacaccgat
     2281 aatgactccc aaaatggtta aaaaactacg caagcgatgg gcattgagcg catctaacgc
     2341 cagaacaatg atttcccgga aattggtccg actggaaaaa ggagaagatg ccataacgag
     2401 tgatgtgcct gacgataaaa tatggctggc gaccaggaat gtctgccatc cgtgccggga
     2461 tcagtgatgc ccaggtaagg gcaaaactgg ttggaactcc agcatgaagt aaagaaaaag
     2521 agacttttgt cgaaaatcgc aatctgagca agtgccggtc tgattgccat tctgaccggt
     2581 ggagagacac aaattttgct cacaacagga aaaaaacagg cacttacaac gtgagaaaac
     2641 tgattctttc agcgctccac gtttcaattt ttgcaatcat ggtctaggat acccaagtct
     2701 gcaaccaatc tgtcattgct atccactggc atcgtctcat cttcgccaac tatttttagc
     2761 aactttaaag agcattcgct atggagtcgc tcgcaccttt ccttgtttgg gggctcaccg
     2821 ggcttctgat tgtgctggtt attggggtgg tgattctgct cgggattcga tcatatactg
     2881 cctggcggga ccggattcgg ctcgatatgc gggatacgac ccgatcaaaa ctcctcaccg
     2941 cgattcgtga attggccgac cccaataaca acggattgac ctgggtcgtt aaacccctca
     3001 aaccaaaatc acaggacgaa acagcggtct tgaagcaagt catgaccgaa ttatcacggg
     3061 tgttgtcgga tgaagaccgc tggcgattgg ccgaattgta tgaaggcacc gggatgcagg
     3121 cagaggatat gcagattctg cgtgatacca aaaaaccctg gtatgagcgg gcacgcactg
     3181 cctaccggct tgggcaaatg
//
LOCUS       DAOQDO010000490         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23433_length_3198_cov_3.327076, whole genome shotgun sequence.
ACCESSION   DAOQDO010000490 DAOQDO010000000
VERSION     DAOQDO010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23433_length_3198_cov_3.327076"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKZ53_09060"
     CDS             <1..445
                     /locus_tag="PKZ53_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40626.1"
                     /translation="DGTCRQGCAIHLPCYQVNTKEIELVAGQQTYRLKRPANFRLNEI
                     HQVDETGKTVLRTWESPIDADVIGLSDDGKALYLLTPIEGVVLELTGGQIRFKVRSKV
                     KYLKGRDVSASATDNKTHTTLQFNLKTTSLWIKYQTPCSLRTQNP"
     gene            666..>3198
                     /locus_tag="PKZ53_09065"
     CDS             666..>3198
                     /locus_tag="PKZ53_09065"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40627.1"
                     /translation="MTAIFSPSNTALRLIIIALVYYGFHKLAFLFLDPVQQVSLIWPP
                     SGFALAVLLLSPKSQLRSTILVLCLVNLFSNYVSSGSIILSFGYLAANLSQAVLGWAV
                     LTSLCTEGIAFTHVSEVLILIGVGTLGTAMTSLIGAGTTVLANQVAFESSYWTWWTSD
                     SLGVLLITPLVVTWTRFRMERPRYSVVEGLFFLTLWCATSWLVFHGIGQVFVPYPYLM
                     MALLVWAVLKFGARGVATAMVILAAIAISGITLTSPFPVLGGKSLTEQLLLVQLYLGV
                     STITGLLLSASLAETKLAEQASREDQLRLRLLGDNLPNGMIYQIVMEPDGSKRFLYVS
                     AGVERLNGVSADMALKDPSALYSLIVEEDSSDLIAAEANSEQTLSPLNAIVRLRRKGN
                     EIRWMQISSSPRRLTDGRILWDGIQLDVTDLILAKENLRQVNRALRTISNCNQVVVRA
                     MTEPELLQNICQVIVENGDYRLAWVGYVQHDEQCSIKPVSYAGVGTDYLETASITWAD
                     GPRGRGPSGVAVRTGQPAVCHNFQTDPAVEPWREQALRYGYQSSLALPLKSGAEVFGV
                     VNVYSAQTDIFHADEIALLVELADDLAYGIMALRARQDHQRVEESLRLSEEHFRSAMY
                     HSPIGKAIVATDGRFLEVNPALCRLLGYTPEELLRLDFQTITHPDDLQPDLGYASQLL
                     NRQIEAYQMEKRYFHKDGHIIWVQLNGSVVWNLDGTPRHFIAQIQDITERRAATLALR
                     ESEERFRQVVENIHEVFWMTDVLKGEMLYISPGYEKIWGRTCESLYQSPETWMDAIHP
                     DDRSRVEQALKKQITGGYMVEYRVIRPDGTIRWVHDQGFPISDTTG"
BASE COUNT          759 a          815 c          816 g          808 t
ORIGIN      
        1 ggacggaacc tgccgccagg gctgtgcgat tcacctgccc tgttatcagg tgaatacaaa
       61 ggaaattgaa ctggtggcgg gccagcaaac ctatcggctc aagcgtccgg ccaattttcg
      121 gctcaatgaa attcatcagg ttgatgagac tggaaagacg gttttgcgga cgtgggagag
      181 tccaattgat gcggatgtga ttggactgtc tgacgatgga aaagcgctgt atctactgac
      241 accgattgaa ggagtggtac tggaactcac tggcggccaa atccgattca aggtccggtc
      301 aaaagtaaaa tatctgaaag gccgtgatgt gtcagcttct gcgactgata acaaaaccca
      361 cacaaccctc cagttcaacc tgaaaaccac atcgctctgg atcaaatacc agactccctg
      421 ttcactacga acacaaaacc cataaggcac ttttcttttc ctcctaacag ttcgcctaca
      481 tttaggttta ctcgacagtc acctttaggg taatctcaat ctctccagca attcaccata
      541 tcccttcctg agtaccacac ctgcaccatt tcagaaaata atctccctcc aggaatgccc
      601 gcaagaggct ggcggcgccc aggggaaata ttgatctcga aatcagttcg aaaaaggcct
      661 gggctatgac cgcaattttt tccccctcaa acactgctct ccgactgatc atcattgccc
      721 tggtctatta tgggtttcat aaacttgcat ttttattctt agacccggta cagcaggttt
      781 cactaatctg gcctccgagt ggatttgccc tggcggtgtt gttgctctca cccaaatcac
      841 agctcagatc aactattctg gttctctgtc tggtcaacct gttttcaaat tatgtgtcaa
      901 gtggctcgat catcttgagt ttcggctatc tggcggccaa tttgtcgcag gcagtgctgg
      961 ggtgggcggt attaacttct ttatgtacgg aaggcattgc gtttacccac gtcagcgaag
     1021 tgctgatctt gatcggagtt ggcacacttg gcaccgcgat gacctcactc attggggctg
     1081 gcaccacggt gctggccaat caggtagcgt ttgaatcttc atactggacc tggtggacct
     1141 ctgatagcct gggcgtgtta ttgattaccc ctctggttgt gacctggacc cggtttcgaa
     1201 tggaacggcc cagatattca gtggttgagg gactgttctt tttgacttta tggtgtgcta
     1261 catcctggct ggtctttcat gggattggac aggtgtttgt tccctatccc tatttaatga
     1321 tggcattgct ggtatgggct gtcttaaagt ttggggcacg gggggttgcc accgccatgg
     1381 tcatactggc agctattgcg attagcggga ttaccctgac ctccccgttc ccggtcctgg
     1441 gtggcaaaag cctcaccgaa cagctcttgc tggtccaact gtatttgggc gtcagtacca
     1501 tcaccggctt gctcctgtca gccagtctgg ccgaaaccaa gctggcggaa caggcatcgc
     1561 gcgaagacca gctccgcctc cgtttgttag gcgacaacct tcccaatgga atgatttatc
     1621 agattgtgat ggaacctgat ggctcaaaac gattcctgta tgtcagcgca ggtgtcgaac
     1681 ggctcaacgg tgtttcggct gatatggcat taaaagaccc gtcggccctg tacagtttaa
     1741 ttgtcgaaga agacagttca gacctgattg ccgctgaggc caattcggaa caaaccttga
     1801 gtcccctcaa tgccattgtc cgactccgcc gtaaggggaa tgaaattcga tggatgcaga
     1861 tttcctcctc gcctcggcga ctgaccgatg gccggatttt gtgggatggg attcaactgg
     1921 atgtgaccga cctcatcctg gcgaaagaaa acctccgcca ggtcaatcgt gccttgcgta
     1981 ccatcagcaa ttgtaatcag gtcgtggtac gggcaatgac cgaaccagag ttacttcaaa
     2041 acatctgcca ggtcattgtg gaaaacggag attatcggtt ggcctgggtt gggtatgttc
     2101 aacacgatga acaatgttcg atcaaaccag tgagctatgc cggagttggt accgactacc
     2161 ttgaaacagc aagtattacc tgggcagatg gtccgcgtgg tcgcggacca tctggagttg
     2221 cagttcggac cggacaacca gccgtgtgtc ataatttcca aactgatcca gccgttgagc
     2281 cctggcgtga acaagccctg cggtacggct atcaatcatc attagccctg cctttaaaga
     2341 gtggtgctga ggtatttggc gtggtcaatg tgtactcggc ccaaaccgat atttttcacg
     2401 ctgatgaaat tgccttactg gttgaattag ccgacgatct cgcctatggg attatggcat
     2461 tgcgcgctcg ccaggatcat caacgggtgg aagaatcatt gcgtttgagc gaagaacatt
     2521 tccgttccgc catgtatcat tccccaattg gaaaagccat tgtggctacc gatggccgat
     2581 ttctcgaagt aaacccggcc ctgtgccggc tgcttggata tactccagaa gaactgctca
     2641 ggctggactt tcaaaccatt acccatcctg acgacctgca acccgacctt ggatacgcca
     2701 gccagttatt aaaccgtcaa atcgaagcct accagatgga aaagcgctat ttccataagg
     2761 atggacacat catctgggtt cagttaaatg gcagcgtggt gtggaacctc gatggaacgc
     2821 cgcgccattt tatcgcccaa atccaggata ttactgaacg ccgggcggcc acgctggctt
     2881 tgcgtgaaag cgaagaacga ttccggcagg tggttgagaa tatccacgaa gttttctgga
     2941 tgacggatgt actcaaaggg gaaatgctct atatcagtcc tggatatgag aaaatttggg
     3001 ggcgcacctg tgaaagtctg taccagtcac ccgaaacctg gatggacgcc attcatcctg
     3061 atgaccgaag ccgggttgaa caagctctga aaaaacagat cacgggtggc tatatggttg
     3121 aataccgggt cattcgcccg gatggcacca ttcgctgggt tcacgaccag ggcttcccga
     3181 tttcagatac aaccggcc
//
LOCUS       DAOQDO010000491         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23469_length_3196_cov_2.354664, whole genome shotgun sequence.
ACCESSION   DAOQDO010000491 DAOQDO010000000
VERSION     DAOQDO010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23469_length_3196_cov_2.354664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..2575
                     /locus_tag="PKZ53_09070"
     CDS             191..2575
                     /locus_tag="PKZ53_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40628.1"
                     /translation="MHPTVRIHNPQPRTRQRAGWILVSTLFAMVCVWGGGGFMYPQTH
                     AHSTSPATQLAATLKMKTDGLPPQFRTGSRDLKPATLPKGTAATVKTARGSFTPSTIP
                     PGTSLEKLTIIGNGFQRDASAFADTDGDGILNSSAFEEFISGERVFSGLITRAITISP
                     KTGRFYVAAADDENKVGVIGIFEMTSDYKARLLRSFDTDQSTAYGLEVVNTAAGDSLI
                     VASTTYTESLLNTDRSDAFSLIVYPPAAGGFPDGNDGKVIIAPKTRLANDNRFVVAFS
                     TGAMTQDGKGNLIINLGLKDSTQFNGGFFAVIRDTNNDGVPDSFNASQPEFFESFDGF
                     VAGNFSNSIVSVPGPGSGRTYYIYSPIAFGSGSAAQSGIVALVDADGDLRADAAPTGL
                     FLASPTNSDLGDGIIFGGAIQIFGRMVFASDNLFYSYFTQEGSDITGMNLNATKIAGG
                     TVGTTVTPLNYSLDGDEADLITFVGVAATGDSVPPTIQVTSPNGGETFQSGTELAISW
                     TSSDDKGVAAHDIALSNDGGGAFPFVVATGLAGSAQSFSYPIPGALETPNARIQVTAK
                     DAGGNVASDTSDSDFVIQIGSSADTQPPSVTVTAPTAGATLNGGRSATVSFTSTDNVG
                     VISHTIAFASDGTTFGTTLVTGLPGSASSFDFTVPNQATTTGVIRVRAFDGAGNNGDG
                     LSGAFTIASDTVKPTVTVTSPGASTKKVKRGDPINVAWTSTDNGTIAKHDVLLSTDDG
                     ATFGTSLATGLAGTAQSFTATAPSSKVKKAIIKVIATDAAGNAGEGLSTKFKVK"
     gene            2973..>3196
                     /locus_tag="PKZ53_09075"
     CDS             2973..>3196
                     /locus_tag="PKZ53_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40629.1"
                     /translation="MRFNTKPPIETGSQLQPRFLFRLIVSFLGAAVLFIPMLLGQTGV
                     NASTQSTHQPYPLDASVHFRLSDKKTNFHA"
BASE COUNT          766 a          837 c          791 g          802 t
ORIGIN      
        1 aaacagattt tatccaaact cattccaaac ctgaccaaat tgttctcttt caactctttc
       61 gacctatatc ccttattttc atcgttcgct taccacctta tggtagaacg cctgccctgg
      121 ctgcgaattc taccggcagg tgtctggcac cgcggtactt gttcaccctt aattcctgga
      181 ggatttcttc atgcacccca cagtacgcat ccataaccca cagcccagaa cccgtcagcg
      241 agccgggtgg attttagtat ccaccctgtt tgcgatggtt tgtgtctggg gaggtggggg
      301 ttttatgtac ccacaaaccc atgcccattc cacttcgccg gcaacccagc ttgccgcaac
      361 tttgaaaatg aaaaccgatg ggttgccacc acagtttcgc accggatccc gcgatttgaa
      421 accggcgacc ttgccaaagg gaactgctgc gactgtcaaa acggcacgcg gttcgtttac
      481 cccgtcaacc atcccaccag gcacctcgct tgagaaattg acgatcattg gcaatggttt
      541 tcagcgggat gcgtcagcct ttgctgatac cgatggagat ggcatcttaa actccagtgc
      601 gtttgaagaa tttatttctg gagaacgggt tttctctgga ttgatcaccc gcgcaatcac
      661 cattagccca aagactggac gcttttatgt ggcggcagcg gatgatgaaa ataaagtcgg
      721 ggtgattggc atttttgaaa tgaccagtga ttataaagcc agattgctcc ggtcgtttga
      781 taccgaccag tcgaccgcct atggcctgga agtggtcaac acggcagccg gtgatagcct
      841 gattgtcgcc agcaccacct ataccgaatc attgttaaac actgatcggt cagatgcttt
      901 ttcactgatt gtgtacccgc cagccgctgg cggatttcca gatggcaacg atggaaaagt
      961 gattattgcc cccaaaacca gactggccaa cgataatcgc tttgtggttg ccttctcaac
     1021 gggggcgatg acgcaggatg gaaaaggaaa tctgattatt aacctgggat tgaaagacag
     1081 cacccagttt aatggcggct tctttgccgt gattcgtgat accaataacg atggtgtccc
     1141 ggatagcttt aacgccagtc aacctgaatt ttttgaaagc tttgatgggt ttgtcgccgg
     1201 gaatttctca aacagtattg tttccgtccc aggacctgga agcggacgaa cttattacat
     1261 ttattcgccg attgcctttg gttcaggcag cgctgcccaa tccggaattg tggcgctggt
     1321 tgatgctgac ggggacttgc gagctgatgc cgcaccaacg ggacttttcc tggcatctcc
     1381 gaccaattcc gatctgggag atgggatcat ctttggtggg gccattcaga tttttggccg
     1441 catggtgttt gccagcgaca atctcttcta cagctacttt acccaggaag gcagcgacat
     1501 caccggcatg aatttaaacg ccaccaaaat cgccggtggt actgttggca ccaccgtgac
     1561 cccgctcaat tattcattgg atggggatga ggccgatctg attacctttg tcggcgtggc
     1621 tgcaactggt gattcagtac ctccgacaat ccaggttact tcacccaatg gtggtgaaac
     1681 cttccagagc gggaccgaac tggcgattag ctggacctca agcgatgaca aaggggttgc
     1741 cgcacacgac attgccttat caaatgatgg cggcggggcg ttcccatttg tggttgccac
     1801 cggactggcg ggttcggctc aatcctttag ttacccaatt cctggggcgc tcgaaactcc
     1861 caatgcccgg attcaggtta cagccaaaga tgcgggtggg aatgtcgctt cagacaccag
     1921 cgatagtgat tttgtgatcc agattggttc cagtgctgat acccagcctc caagtgtgac
     1981 cgtgactgcg ccgacggcgg gtgccaccct caatggtggg cgatctgcca cggtgagctt
     2041 tacttcaact gacaatgtgg gtgttatttc acacaccatc gcgtttgcct cagacggcac
     2101 gacctttgga acaactctgg tgactggctt accaggcagt gcttcgtcct ttgattttac
     2161 agtgcccaat caggcgacaa ccacgggtgt gattcgggtg cgtgcttttg atggggctgg
     2221 gaacaatggt gacgggttga gcggtgcctt tacgattgct tctgacaccg tcaaaccaac
     2281 ggtgaccgtg acctcacctg gggcgagcac caagaaggtc aagcgcggcg acccgatcaa
     2341 cgtggcctgg acttcaaccg acaacggaac gattgccaaa cacgacgtgt tgctgtcaac
     2401 cgacgatggg gccacgtttg gaacctcact cgcaacggga ctggctggaa cggcgcaatc
     2461 gtttacagcc acggccccaa gctcgaaagt caagaaagcg atcatcaagg tgattgccac
     2521 cgatgcggct gggaatgctg gtgaaggtct ctcgaccaaa tttaaggtca agtaactcgg
     2581 tagtgttttc gcaaaccggc aggatctctc tgcatcaatt ccatagagag gggtccagga
     2641 aagcctaaat cccggtggaa tttgtattgg tacagaatac aactcccaac agaaaaagag
     2701 gttgcaagca aaatctgctc gcaacctctt tacctttttt gctactcttc aaaaacgtcc
     2761 tcaaacaccg ttttcggttc agtgtctgaa ccagttcact ctaaattcaa atcaaactac
     2821 aaccacgcct tcgctttttt gttgagaagt taacgcataa cctcgacgtc caaacctcat
     2881 tgctagtttc cgattggggc tcaccctgac aggcggatga ctggcaagtg agggaaagcg
     2941 atgaagtgtc tcaccaagga ggagttttcc cgatgagatt taataccaaa ccacccattg
     3001 aaacaggaag tcaacttcag ccaagatttc tattccgcct gatcgtaagt tttttgggtg
     3061 cagccgtcct cttcatccca atgttattgg gacaaaccgg agtaaatgcc agtactcaat
     3121 ctactcacca gccttatcca ttagatgcct cagtgcattt tcgactttct gacaaaaaaa
     3181 cgaattttca cgcttt
//
LOCUS       DAOQDO010000492         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23476_length_3195_cov_3.253822, whole genome shotgun sequence.
ACCESSION   DAOQDO010000492 DAOQDO010000000
VERSION     DAOQDO010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23476_length_3195_cov_3.253822"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKZ53_09080"
     CDS             complement(<1..716)
                     /locus_tag="PKZ53_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ40630.1"
                     /translation="MSQPRIICIASQKGGVGKTTIASNLGAVLATTHRVLLIDADPQG
                     NLTQGLSLTPDTLETTLYHVLVKNWPMEKAMLTPLEALPNLKLIGANLDLAAAEIELL
                     GKVGADTRLRKALQTLATPVDFIIIDTPPSLATLTLNALVAAHEVLIPVDPGVYGLYG
                     LSKLVELIDDVRSYNSELGRVRAVRCRVDHTNLSESVRLNLENAFGEDLFKSTIRKSV
                     RIGESQAASLPVILYRPNDAV"
     gene            981..2354
                     /locus_tag="PKZ53_09085"
     CDS             981..2354
                     /locus_tag="PKZ53_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNJ40631.1"
                     /translation="MNLVKTTFLLLIFSLVWSSSGCKSFEPPPSEFPESLQKVWRYPI
                     ANGSGPYLIGKGPLVLVFDSLGTATALDARTGKERWTLKTLDKASLSPDVWEGLLFIT
                     SYDGGGISAGSKYTTQCVEAETGKELWHFDQWQTYFPLLRNGMIVGQLAEKEKQIPHQ
                     EFTERISVCVGLDAKTGKELYRTEFNRGEWEKNHPQERLDAHIVGENGQMLMVNSTKN
                     VPVIDFARQKIETRMFDYAGETPDKYSIIGNTLLVISFNWIKNEYKDFSAKYRDLYLQ
                     AFDIETGKTLWTKKKSAKFYDLAKGKPGVVLVEDWEGNLMAYEVATGKELWPKPIFTG
                     SVQHVTLDDVAENTLLARPGESSEWFLAYNATTGEKLWEQKTDDLNRTFWLYTHRGVA
                     YVESTQAGTRFNSPVTLNTRLAAYDLKSGKKLWHILNYDLGNVVCFEDVILIGMKSEV
                     FAVKAGQ"
BASE COUNT          838 a          724 c          832 g          801 t
ORIGIN      
        1 acggcatcat ttgggcggta caaaatcaca ggtaaactgg ccgcctgtga ttcgccaatg
       61 cgcactgatt tgcgaatggt tgacttgaaa aggtcttctc caaaggcatt ttcaaggttg
      121 agccgcacac tttccgacaa attggtgtga tctacccggc agcgaaccgc ccgcacccga
      181 cccagttctg agttgtagct ccgcacgtcg tcaattaatt caaccagttt ggacagacca
      241 tagaggccgt acacccctgg atcaaccggg atcaggactt cgtgggcggc caccagggca
      301 ttgagggtca acgtggcgag cgatggcggc gtgtcaataa taatgaagtc aactggggtg
      361 gccagagttt gcagggcttt gcggagtcga gtatcagcgc cgacctttcc cagtaactca
      421 atttcagccg cggccagatc caggttggcc ccaataagct tgaggtttgg cagggcttca
      481 agtggcgtca acatggcctt ttccatcggc cagtttttga ccaatacgtg atagagcgtg
      541 gtttcaagcg tgtcaggcgt gaggctgagc ccctgggtca ggtttccctg tggatcggca
      601 tcaatgagca atacgcggtg ggttgtggcc agtacggcgc ccagatttga ggcaatcgtg
      661 gttttgccaa cgccaccttt ctggctggca atgcaaataa ttcgtggttg agacataagg
      721 cggtagcttt tcagcttgtg gagaatccgt gtatggtagt ctggggtttg gacgaaataa
      781 taccattagg gttcagggtt cagggttcag ggttcagggg aatccaattc tttcaatgac
      841 ttagccgttt tctacgatgt agtgtttatg gcaaaactca tccttcatca ctgaatggaa
      901 ttgattgact ggaccggttt atagcacttc tttgcgctca attgctattc ctatagcgcc
      961 ttatcatttg gagaacatca atgaatcttg tgaaaacaac ctttcttctg ctcatttttt
     1021 ctctggtctg gagcagtagc ggctgcaaat cgtttgagcc accaccctca gagtttcctg
     1081 aatcacttca aaaagtttgg cgctacccaa tcgccaatgg ttcagggcca tatttgattg
     1141 gaaagggccc actggtgctc gtttttgaca gcctgggaac ggcgacggca ctggatgccc
     1201 gaactgggaa ggaacggtgg acactcaaaa ccctcgacaa agcctcgctg tcacccgatg
     1261 tctgggaggg attactcttt atcacgtcat atgatggtgg cggaatcagc gccgggtcga
     1321 aatatacaac gcaatgtgtt gaggctgaaa ccggaaagga actctggcat tttgaccagt
     1381 ggcagaccta tttcccactt ttgcgcaacg gcatgatcgt tggtcagctc gctgaaaaag
     1441 agaaacaaat tccacaccaa gagtttactg agcggatttc ggtatgcgtc ggactggatg
     1501 ccaaaacagg gaaagaactc tatcgcacgg aatttaatcg cggcgaatgg gaaaaaaacc
     1561 acccacagga acggcttgat gcccatatcg tcggtgaaaa tggccaaatg ttgatggtga
     1621 attcaaccaa aaatgttcca gtgattgatt ttgccagaca aaaaattgaa acgcgcatgt
     1681 ttgactatgc gggtgagact ccagacaaat actcgatcat tggcaacaca ctgctggtca
     1741 tcagcttcaa ctggatcaag aacgagtata aggactttag tgccaaatac cgggacctgt
     1801 atttgcaggc gtttgacatc gaaaccggaa aaacgctctg gacgaaaaag aagtccgcca
     1861 aattttatga cctggcgaaa gggaagccag gcgtggtgct ggtggaggat tgggaaggca
     1921 atttgatggc ctatgaggtt gccaccggca aggaactctg gccaaagcca atttttaccg
     1981 ggagcgtcca gcacgttacg ttggatgatg tcgctgaaaa cacgctcctg gctcgacctg
     2041 gagaatcgag tgaatggttt ctggcctata acgcgaccac cggggaaaag ctctgggagc
     2101 aaaaaacgga tgacctcaat cgaactttct ggctctacac ccatcgcggc gtcgcctatg
     2161 tggaatccac ccaggctgga acgcggttta attctccggt gaccctcaat accagattgg
     2221 cggcctatga cctgaaatct ggaaaaaagc tctggcacat tctcaactat gatctgggga
     2281 acgtggtctg ctttgaagac gtcatcttga ttgggatgaa gtcagaggtg tttgcggtca
     2341 aggctggcca atgaagggtg aagtcgcgaa gtagctggat gaatctttgc agaaggtgat
     2401 tgaatctatg gatttcgccg cgttctgata attaattggc cggttttttc atcatgactc
     2461 aattattgat atgtttgttt atcttgaatt taagattgat tgaggaagaa aaagacttcc
     2521 cgtgcggtaa atcagcctga gcggcttaat tcttcaatca gggatcgaaa atgcaccctt
     2581 tatgagagcg tagggcggtt ctttctcgcc gaagcgtccc aggtgcgcta ctcttgaagt
     2641 gaactttctg gttcatcatt gaaccagtat gaacccacgc gctccacaag gcgcgtgcct
     2701 gttaacgtcc aaatcaaaaa cacatggagg accccatggc caaagggaac aacagccaac
     2761 ggaaagagac caagaaaccg aaaaaagaca aacccaaaaa agggtcgcca gaaccaaaga
     2821 aaggataggt ggtttggctt agatacctgg tttgaacttt gatctaaagt acagaggagg
     2881 gccatctgca ctgtttgcgg atgccctcac ttttccagca cctcaaccac aatcaaacca
     2941 gtttgctctt tgcacaactg aaagactcga agcaagaaaa atcacttcaa tcgctgtcgt
     3001 gtggactggg gatggtttgg tatggtgttt ataccagttt gtagtcagta gcccgtagtc
     3061 agtagttgac taagcttatt tgattgaatc atttgactgc tttctcatac gaggattcca
     3121 ttccaaattg gtattatttt tttgagagag gaacctctaa gggttcatgc cattctttaa
     3181 ggtcaatgag ccatg
//
LOCUS       DAOQDO010000493         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23505_length_3193_cov_3.422881, whole genome shotgun sequence.
ACCESSION   DAOQDO010000493 DAOQDO010000000
VERSION     DAOQDO010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23505_length_3193_cov_3.422881"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKZ53_09090"
     CDS             <1..653
                     /locus_tag="PKZ53_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ40632.1"
                     /translation="IKTVVAQLTSASLFESMHPDAADFFSDVGIPIVLPERQDEGSEK
                     RLLELLHESISQLKNPDETTEVSLLLMRLLAECFDRALFFLVRGDQLIGRGGFGFSAA
                     AEKATAKVRKLRIPLAESDTFGQVCAQGKPFRGNLNPHWWVKHIPVEVGECPSREIVI
                     YPLQTQGGTIGLVYADNSIHHKPLSNTHVLEIFLFQAGIALENRNLQQRLMGRSAS"
     gene            886..3057
                     /locus_tag="PKZ53_09095"
     CDS             886..3057
                     /locus_tag="PKZ53_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNJ40633.1"
                     /translation="MTTTPVSHFRTCNLCEALCGIEITVNNQEIVSIQGDRQDPFSRG
                     HICPKAVALKDVHLDPNRLRQPVRRTPTGWEPISWETAFDEVATRLRSVQAQHGVNAV
                     GVYLGNPNVHNYGSILFGPLMLKALGTKNRFSATSVDQLPHHFSGFFLYGHQLMLPIP
                     DIDRTDFLLIVGGNPVVSNGSLMSSPDVADRLKEIRQRGGKVIVVDPRRTETAAIADE
                     HFFIRPGTDVYFLLGMLNTLLVENRVQMGRLAEFTDNLDQLRPLVEGYSAEVVADVTG
                     IPAEAIRRLARDFAAAPSAVCYGRMGVSVQEFGATAQWLIQVFNIVTGNLDRPGGAMF
                     TLPAFDAVDLTGRMGRKGHFGKWKSRVRNLPEFGGELPVVAMAEEMLTEGPGQIRALV
                     TSAGNPVLSTPNGSQLETALAGLDFMVSVDFYINETTRHAHIILPPAAGLEHENYDVV
                     FHVLAIRNTTKYTPALFPADSNSRHDWEIFLELFLRLGGKHIPEKEQRRIKRWLAKRN
                     APEVVLDWALRNGPYGSRFSPISQQLTLHKLKQHPHGIDLGALQPCLPHRLFTQDKRI
                     NLAPEVFINDLARVERRFSEITQETSSDQLWLISRRHLRSNNSWMHNAVRLVRGKDRC
                     TLLMNPQDAENRQIQPGQQVAISSRVGTVQAKVEITAEMMPGVVSLPHGWGHHREGIQ
                     MDVAQKFAGVSVNDLTDDSRLDELSGNAALSGVPVSVVRIG"
BASE COUNT          746 a          797 c          860 g          790 t
ORIGIN      
        1 atatcaagac cgttgtcgct caattgacca gtgcctcact ttttgaatca atgcatccag
       61 acgcggctga tttcttttca gatgttggga ttccgattgt tttgccggaa cgtcaggacg
      121 aagggtctga aaagcggttg ctcgaactcc tgcatgaatc catctcccag ttaaaaaacc
      181 ccgacgaaac cacagaggtt tctctgttgc tcatgcgact cctggctgaa tgctttgacc
      241 gggcactttt ctttctggtc aggggggatc agctcattgg acggggtggt tttgggtttt
      301 cggcagcggc tgaaaaagca accgccaaag ttcgcaaatt gcgcattcct cttgctgaaa
      361 gcgatacctt tggtcaggtg tgtgcgcagg gaaagccgtt tcgcggcaat ctcaatccgc
      421 actggtgggt taaacatatt ccagttgaag tcggtgagtg tccttcacgt gaaatcgtga
      481 tttacccgtt gcaaacccag gggggaacga ttggacttgt gtacgctgac aactcaatcc
      541 accacaaacc actttcaaat acccatgtgc ttgagatttt tctgttccag gctgggattg
      601 cccttgaaaa tcgcaatctg caacaacgcc tgatgggccg ctcggcaagc tagacaggaa
      661 gacatcgggc ttggggctga gggcttgggg cgattgaagg gatgagggat gaaggatgag
      721 ggatgaaaca aaaccacttc ttcagcccaa tgtcttcagc ccccagccct gagccctggt
      781 tcttcagccc cgagtcttcg agtcttcagc cccaaaccct cagcccaatg tcttcagccc
      841 aatgtcttca gcccaaactt ctaatcccgc acttccaaat attttatgac caccacgcct
      901 gtatcacatt tccgaacctg taatttgtgt gaagccctgt gtggcatcga aatcacggtg
      961 aacaaccagg aaatcgtttc gattcaaggt gatcgccagg atccatttag ccgtggccat
     1021 atttgcccca aggccgtggc gctcaaggat gttcacctgg atccaaatcg gctgcgccaa
     1081 ccagttcgcc ggactccaac cgggtgggag ccaatcagtt gggaaaccgc ttttgatgag
     1141 gtggccaccc gcctgcgaag tgttcaggcc cagcatggcg tgaatgcggt gggggtatac
     1201 ctgggaaatc cgaatgttca caactatggt tcgattctct ttggtccgct catgctcaaa
     1261 gcattgggga ccaaaaatcg cttttcagct acgtccgttg atcaattacc acatcatttc
     1321 tcagggttct ttttgtatgg tcatcagttg atgctcccga ttccggatat tgaccggaca
     1381 gatttcctgt tgattgtggg cgggaatcca gtggtctcaa acggaagttt gatgtcgtcg
     1441 cctgatgttg ccgaccggtt aaaagaaatc cggcaacgcg gtggaaaagt gattgtggtt
     1501 gatccccgcc ggactgaaac cgcggcgatt gctgatgaac atttctttat tcgcccagga
     1561 acggatgtct attttctgct ggggatgctc aataccttgc tggttgaaaa ccgtgtccag
     1621 atggggcgac tggctgaatt taccgacaac ctggaccagc ttcgcccgct ggtcgaaggc
     1681 tattcggctg aagtcgtggc tgatgtaact ggtattccgg ctgaggctat tcggcggctg
     1741 gcacgagact ttgccgctgc accttcggcg gtgtgttatg gtcggatggg ggtttcggtc
     1801 caggaatttg gcgccaccgc tcagtggtta atccaggtat tcaacattgt cactgggaac
     1861 ctggatcgcc caggcggagc gatgtttacc ttgccggcct ttgatgccgt agatttaacc
     1921 ggacgcatgg ggcgcaaagg tcactttgga aaatggaaaa gccgggttcg caacctgcct
     1981 gaatttggcg gtgaacttcc agtcgtggcc atggctgaag aaatgttgac tgaaggtccg
     2041 ggacaaattc gggcgctggt gacttcggct ggaaacccag tgctgtcaac gccaaatgga
     2101 agtcagcttg aaacagcact tgctgggctc gattttatgg tgtcggttga tttttatatc
     2161 aatgaaacga cccgccacgc tcatatcatc ctgccgccag ccgccggatt agagcatgaa
     2221 aactatgatg tagtctttca cgtgctggcc atccgaaaca cgaccaaata taccccggcg
     2281 ctctttccgg ctgattcaaa ctcgcgccac gattgggaaa ttttcttaga actgtttttg
     2341 agacttggcg gaaaacatat ccctgaaaaa gagcaacggc ggatcaaacg ctggctggca
     2401 aagcgcaatg caccagaggt tgttttagat tgggcgttgc ggaatggtcc ctatggctct
     2461 cgcttttcac ccatctcgca acaactgacg ctgcacaagc tcaaacaaca tccgcatggt
     2521 attgacctgg gtgcgttaca gccttgcctc ccacaccggc tttttacgca agataaacgc
     2581 atcaatctgg cacctgaggt gtttatcaat gatctggcac gggtcgaacg tcggttttca
     2641 gagattaccc aggaaacaag ctcagaccaa ctgtggctca tcagccggcg tcatttgagg
     2701 agcaataatt cctggatgca caatgccgtt cgactggtgc ggggaaaaga tcgctgcacg
     2761 ttgttgatga atccccagga tgctgaaaac cggcagattc aaccggggca acaggtggca
     2821 atttcttcgc gcgtcggcac cgtccaggcc aaagttgaaa ttactgctga gatgatgcca
     2881 ggggtggtca gccttccaca cggctggggt caccatcggg agggcatcca gatggatgtc
     2941 gctcagaaat ttgccggggt gagtgtcaat gatctgactg atgattcgag acttgatgaa
     3001 ttaagtggaa atgcggcact gagtggggtg cccgtttcag ttgtgcgcat tggttaaatc
     3061 cgattgaata gacgagggga agagggaacc agaaccagaa gtcaaagtcc ttgagccctg
     3121 agtccgttac cagcaaaagc aaaaaggagg agcgatgtcg ctcctccttt tgttttgatc
     3181 aaatctgacc agc
//
LOCUS       DAOQDO010000494         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23524_length_3191_cov_4.764031, whole genome shotgun sequence.
ACCESSION   DAOQDO010000494 DAOQDO010000000
VERSION     DAOQDO010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23524_length_3191_cov_4.764031"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PKZ53_09100"
     CDS             <1..1959
                     /locus_tag="PKZ53_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ40634.1"
                     /translation="MRTWLYEARMEGNIDRAKSLAEKAQRIAVEFGFTRYKTLAKDEL
                     QRRSTQIIIRDGAFTEYFEKLKQELMSSETADQLFQVTGQVLRRRIIGQPIRSFSFLE
                     SDGQDVRVVYKAEEEGEFVSRRHVINSRYILVVPPSAHWVLVEDLVQVLRVRLAQLEN
                     ESISQDVTVIKDDLIVYSSRMDAFVNRCREVIHSPNRAIPILITGETGTGKSWLAEKL
                     HSLCPLTQRGGLQHWFASENRGGDVNMFKSNLFGFVAGFANHLKKDQPGKVQLAQDGS
                     FIMDEIGELELDRQAVLLQLLNNWKYERVGDPTTCQANCRIYLATNRNLKQMVLDGTF
                     REDLYFRISMDNLHVPPLRLFPEQIIPLARDFSRQEIQNFNQVGMIIPKEVIESGIVF
                     TREATERLLNYSWPGNVRQLKLFLRMLVSTLLQKQNYVVNEEVAEQMFSRLQEMDPFS
                     SFVKKDSARISFDQEKNKIRFQEMVTRVFGPPETRTEEQNFLVGLLGRLEPVKVNPSL
                     LLRLERNSKALIEKLRKPRKSNQKSKGKIGFSEIQEICEAYLLMQYYLNLIVYEYKLN
                     DSQLFDQTETLSWPRSFRRLYLNEVFEVTQVNFAEFVGTLRTDQFVEEELIEKFSESL
                     KRYFDLRASYRKKKLDRRKRREKGLEKI"
     gene            2195..2407
                     /locus_tag="PKZ53_09105"
     CDS             2195..2407
                     /locus_tag="PKZ53_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40635.1"
                     /translation="MELYRTQTQSGGLSLRLPLPDSERRSSGVIHPKWKPRTPGIAAG
                     LTDHVWTFREHLTAKFEPIQNHSISR"
     gene            complement(2986..>3191)
                     /locus_tag="PKZ53_09110"
     CDS             complement(2986..>3191)
                     /locus_tag="PKZ53_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40636.1"
                     /translation="WGTPMLIPIHGKVPVTEQILEEYRWSFDARPGCIFKFGEMPDPI
                     HHFSPPPLKKPGIISRIVHSILG"
BASE COUNT          975 a          556 c          791 g          869 t
ORIGIN      
        1 atgcggacct ggctttacga agctcgaatg gagggaaata ttgatcgggc aaaaagtttg
       61 gccgaaaaag cgcaacgaat cgcagtggaa ttcggattta cgagatataa gacgttagcg
      121 aaggatgagt tacagcgtcg atcaacacag attattattc gagatggggc gtttacagag
      181 tattttgaga agttgaagca ggaattgatg agttcagaaa ctgcagatca gctttttcaa
      241 gtaacaggcc aggttttaag gcgtcgaatt atcggacaac ctattcgcag tttttctttt
      301 ttggaaagtg atggacagga tgtgcgggtg gtttataaag cagaggaaga gggagaattc
      361 gtgagtcgcc ggcacgtgat taatagcagg tatatcctgg ttgttccacc atctgcccat
      421 tgggtattag tggaagacct ggtacaagtc cttcgggtca ggttggcaca acttgaaaat
      481 gaatctattt ctcaagatgt gacggttatc aaagatgatt tgattgtgta ttcctcacga
      541 atggacgctt ttgtgaaccg gtgtcgagaa gtaattcatt cgccgaatcg tgctattcca
      601 atcttgatca cgggtgagac aggaactgga aagtcctggt tggcggagaa actgcattca
      661 ttatgtcctc tcacccaacg tggtggatta cagcactggt ttgcttctga gaatagaggt
      721 ggcgacgtca atatgtttaa gtctaacctg tttgggtttg ttgccggttt tgcaaaccac
      781 ctgaaaaaag atcagccggg taaagttcaa cttgcgcagg atgggtcgtt cattatggat
      841 gagattggtg agcttgaact ggatcgtcaa gcggtgttgc ttcaactgtt gaataactgg
      901 aaatatgaac gagttggcga tccgacaact tgtcaggcga attgtcgcat ttatttggct
      961 acgaaccgga atttaaaaca gatggtgctg gatgggactt ttagagaaga tctttatttt
     1021 cggatttcaa tggataatct tcatgttccg ccactcagat tatttccaga acaaatcata
     1081 ccccttgcac gtgacttcag ccgacaagag attcaaaatt tcaatcaggt cgggatgatt
     1141 atcccgaagg aagttattga aagtgggata gtgtttacac gtgaagcaac ggaacgactg
     1201 ctgaactatt cctggcctgg aaatgttcgg caactgaagt tgtttttgcg aatgttagtc
     1261 tcaactcttc ttcaaaaaca aaattatgtt gttaatgaag aggtggcaga gcaaatgttt
     1321 tctcggttgc aggaaatgga tccattttct tcattcgtaa aaaaagattc agccaggata
     1381 agttttgatc aggaaaaaaa caaaatacga tttcaggaaa tggttacccg agtttttggg
     1441 ccaccggaaa cacggactga agagcaaaat tttttggttg gtcttctcgg tagattagaa
     1501 ccagtgaagg taaacccatc tttgttattg agactcgaaa gaaatagtaa agctttgatc
     1561 gaaaaactaa gaaaaccaag aaaatcaaat cagaaatcaa aaggtaagat tggtttttca
     1621 gaaattcagg aaatctgtga agcctattta ttgatgcaat attacctgaa tttgattgtt
     1681 tatgagtata aactcaacga tagccaactt tttgaccaga cggaaacatt gagctggcct
     1741 cggtcattca gaagattgta tttgaatgaa gtgtttgagg taactcaagt caacttcgca
     1801 gagtttgtcg gtacattaag gacggatcag tttgtagaag aagagcttat cgaaaagttt
     1861 tctgaaagcc tgaagcggta ttttgattta agggcgtcct atcggaaaaa aaagcttgac
     1921 cgccgcaaaa ggagggagaa aggtctggaa aagatttgac caaatcccaa atttgaagca
     1981 tccggggtgc tcatcggcta atattttgaa aaagaaacag gccagtccga cgctagaaaa
     2041 gacaaaaaag agcagagaaa tccgaatcag ttttgtgatg ggtcaggtca ggaacggtgt
     2101 atgagtggtt gaggagagca gcggaaaaag ctgaggaggt caaccagcat ctaatacggg
     2161 aattgggagt gagccaggtg caactggatg aaatgtggag ctttatcgaa cgcaaacaca
     2221 atctggaggc ttgagtctgc gtctccctct gcctgactct gagcgtcgtt cctcaggtgt
     2281 gattcatccc aaatggaaac cccggactcc agggattgcg gctgggttaa ccgaccatgt
     2341 ctggaccttt cgcgagcatt taaccgccaa atttgagccg attcagaatc acagtattag
     2401 ccgatgagca ctaaatctat caaattataa aaaaacaatt caaaataggg ttttggtata
     2461 ttaattgcag atataaaagc acgtaaaata tatcaacact ggagcgacat atggaaaaat
     2521 tgattgaaca tgatggtacc acaattggat tgaatcaaac tatcacccga gtgaggggtg
     2581 gtgggttcgg atccgatgcc tgggttgcca aaattgttgg aattgaggat gatggcaaat
     2641 tgaaacgaga attttgcaga aaagataaaa caaacctttc tggatcaggg agatctggaa
     2701 caatagaatt tacagttgat gaattcggaa tctatgaatt ccggaatttc tgccggaaca
     2761 cacatcccat gcgctggcgc tggtcgggat tttttgaaat tttgggcgat ggttcagtgg
     2821 aattgattga tcagaaagaa cttgttcgac ggttcactga ttattgctgg aaataagtgg
     2881 aaaaatctca aacgagcgaa tacaaaattt gtctctaaca agagttgttt taaataaaag
     2941 gactgataaa ttgaacattc tatcagtcct tttttttcgt gaatattatc ccagaattga
     3001 gtgtactatt cgggaaataa tgcctggttt tttcaatgga ggtggagaaa aatgatgtat
     3061 cgggtccggc atctcaccaa atttgaaaat acaccctggc cgggcatcaa agctccagcg
     3121 atattcctct aaaatctgtt cggtcaccgg aactttgccg tggatgggga tcagcatcgg
     3181 cgtcccccag t
//
LOCUS       DAOQDO010000495         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23538_length_3190_cov_3.242105, whole genome shotgun sequence.
ACCESSION   DAOQDO010000495 DAOQDO010000000
VERSION     DAOQDO010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_3190_cov_3.242105"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKZ53_09115"
     CDS             <1..795
                     /locus_tag="PKZ53_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNJ40637.1"
                     /translation="VEMSQQPEEIRHKTDRLDAVGNTTKALTKGYAVGSAALAAFLLF
                     SAYMDEVKNYGLKLERIDLSKPEVFTGALLGAMLVLLFSAMAIKAVGSAASAVISEVR
                     EQFKKKPGILKGTEKPDYGHCVDIVTKSALREMVLPGLLVVLSPIVVGVLFKYLYQSQ
                     GGGLVDPEVSRLATGAEVVGGLLMVGTIVGILMALYLNNAGGAWDNAKKYIETGQFGG
                     KRSDTHKATVVGDTVGDPFKDTAGPSLHVLIKLLSTITLVMAPLFI"
     gene            986..2389
                     /locus_tag="PKZ53_09120"
     CDS             986..2389
                     /locus_tag="PKZ53_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ40638.1"
                     /translation="MNTAHRFSFEPGTIGFGLFGFPVRIEWTFWLVTLLMGAMRQPGA
                     LYMAEWTVVVLVSILIHELGHALCSAWYGMKPEIKLYGMGGLTTFTPPEPMPPSKNIL
                     IDLGGPIAGFLFAGLIVVAALMLPGWRQSEFGKVVFFDLLWVNVGWGLLNLLPVWPLD
                     GGQVVWELERMFSRKATGIVTEIVSCVVCLALIAVALGTQFRTAVVIILWFGFPNFQA
                     LFNRFQQKQEKRLWDQLSEIKGHIENHQPSQAIAQALTLWEQASSQDLKQQVLWLLID
                     AHFENKTFDDGWLFVLRYKVLFGPLPDYEGAYLVELKQPERAIRILKPEFEKHPQSWA
                     TSYYLLALAQLGQVAEVLDLCQNLTPPQEIIRVLALLQTEMFFQGNYADSIKIGKRAL
                     EVGGPGNFAYNIACACSKLGELDLGIEWLVKAIDLGFTSPDSFDADPDIESLRQSPGY
                     ERVVRALQAAVENPKDD"
     gene            2557..>3190
                     /locus_tag="PKZ53_09125"
     CDS             2557..>3190
                     /locus_tag="PKZ53_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40639.1"
                     /translation="MKKAVYLLFLMFVLHPGWFNQPLLAQDEPLVEPEKKSWVESTGA
                     RQAVVVDAGLAALRKSPTSQGELKQRLRVGRKLYVLSQTRNRNGQVYYWVAVTRRTRG
                     WIDARALVRSGTRGDDGRLWKLILAESNHFEQLRLCQIFFSLFKQSKFTPQVLWQLAE
                     TAQAEAKTLNSRIQRRAKEAATSENADLATYVENDVGLDRYNRLGIIFLFH"
BASE COUNT          746 a          762 c          874 g          808 t
ORIGIN      
        1 gtggaaatgt cacaacagcc cgaagaaatc cgtcacaaaa ccgaccgtct ggatgcggtg
       61 gggaacacaa ccaaagcgtt gaccaaagga tatgccgtgg gctcagccgc actggccgcg
      121 ttcctgctgt tttccgccta tatggatgaa gtcaaaaact acgggctgaa gctggaacgc
      181 attgatcttt ccaagcccga agtcttcacc ggcgccctgc tcggagcgat gctggtatta
      241 ttgttctcag cgatggccat caaggccgtg ggttcagcgg cttcagccgt catctctgaa
      301 gttcgagaac agttcaagaa gaagcccggc attttgaaag gtacagaaaa acccgactac
      361 ggccactgcg tagacatcgt gaccaaatcg gccttgcgcg aaatggtgtt gccaggcttg
      421 ctggtggtgt tgtctccgat tgtggttggt gtgctgttta agtacctcta tcaaagtcag
      481 ggtggaggtc tggttgatcc ggaagtgagc cggctggcca caggtgctga agtcgttggc
      541 gggttgctga tggtggggac gattgttgga attctgatgg ccctctacct caacaacgct
      601 ggcggtgcct gggataacgc caaaaagtac atcgaaaccg gccagtttgg cggcaaacgc
      661 agcgacactc ataaagcaac tgtcgtgggc gacaccgtgg gcgatccatt taaggatacg
      721 gctggtccgt cgcttcacgt gctgatcaag ttgctgtcaa ccatcacgct ggtgatggcg
      781 ccgttgttta tctaagcgtc gaaaggctca accaaaagta gacatcatca ggttgaaggt
      841 tgttggttcc acctgagttt tatcctgttt catttttctt cccgaatttt gagggaatcg
      901 tccacccagc caggtcacac tgcggtccgg taccgctttg gaaactggct gggtcttttt
      961 gtatcaattt tcgaatggca cgaacatgaa caccgcacat cgcttcagtt ttgagccggg
     1021 gacgattgga tttggactgt ttggttttcc agttcgaatt gagtggacgt tctggctggt
     1081 gacactcttg atgggggcca tgcgccagcc gggagcactt tacatggccg aatggacggt
     1141 tgtggtgctg gtctcgattc tcattcatga acttggccac gcgctctgta gcgcctggta
     1201 tggaatgaaa cccgagatca aactctatgg gatgggaggg ctgaccactt ttactccacc
     1261 tgaacccatg cctccatcca aaaatatact gattgacctg ggtggaccaa tagccggctt
     1321 tttatttgct ggattgattg tcgttgcggc cttgatgctg cctggctggc gtcaatctga
     1381 atttgggaag gtcgtgttct ttgatttatt gtgggtcaat gttggctggg gactgctgaa
     1441 cctgttgcca gtctggccgc tggatggagg tcaggtggtt tgggaactgg agcgaatgtt
     1501 ttcgcgcaag gccacgggga tcgtgaccga gattgtgtca tgtgtggtgt gcctcgcctt
     1561 gattgccgtg gcgcttggaa ctcagtttcg aaccgctgtg gtcatcatct tatggtttgg
     1621 ttttccaaat tttcaggcac tcttcaatcg ttttcagcaa aagcaggaaa aacgattgtg
     1681 ggatcaactc agtgaaatca aagggcatat cgaaaaccac cagccatccc aggccatcgc
     1741 ccaggccctg accctttggg aacaggcatc gagtcaggat ttgaaacaac aggtgttatg
     1801 gctgctgatt gacgcccatt ttgaaaacaa gactttcgat gatggctggc tgtttgtcct
     1861 gcgatataag gtcttgtttg gcccgctgcc tgactatgaa ggtgcctatc tggttgaact
     1921 gaagcagccg gaaagagcca ttcgcattct caaaccggaa tttgaaaaac atccccaatc
     1981 ctgggccacc agttactacc tgctggcact ggcacagcta ggacaggtcg cagaggtact
     2041 tgacctgtgt cagaacctga ccccgccaca agaaatcatt cgggtgttag cccttcttca
     2101 aaccgaaatg ttttttcagg gaaactatgc ggattcgatc aaaatcggaa agcgggcgct
     2161 tgaagtggga ggaccaggaa attttgccta taacatcgcc tgtgcctgtt ccaaacttgg
     2221 cgaactggat ctgggaattg aatggctggt gaaggccatt gatctgggat ttaccagccc
     2281 ggacagcttt gatgccgacc cggatatcga atcgctccgg cagtctccag gctatgaacg
     2341 tgtggtgcga gcattacagg cggcggtcga gaatcccaaa gacgactaat ccaggaaatt
     2401 cattcggtat ggaatttgcc ctttgatcac tcagaagata gatcaagtgc tgcaatacgc
     2461 tgagatttga atgtaaattc ttcattttca gttggttgcg ttttaattga tcttgagttc
     2521 aatcaaaaat tgatccaaag ggaaatactg aatgatatga agaaagctgt gtatctgcta
     2581 ttcctgatgt ttgttctcca tcctggctgg ttcaatcaac cgttgctggc acaggacgag
     2641 ccactggttg aacccgaaaa aaaatcctgg gtggaatcaa ccggcgcccg acaagctgtg
     2701 gtcgttgatg ccgggctggc ggcactcaga aaaagcccaa cgtcacaggg cgaactcaag
     2761 caacggttgc gggttgggcg caagctctat gttttatcac aaacccgaaa tcgaaatggc
     2821 caggtatatt attgggtagc ggtcacccgt cggacgcgtg gctggattga tgcccgggcc
     2881 ctggtacggt ctggaacccg tggggatgat ggccggcttt ggaagctcat tttggccgag
     2941 tcaaaccact ttgagcagtt gcgcctctgt cagatatttt tcagtttatt caaacaatcc
     3001 aaatttacac ctcaggtgct ctggcaactg gccgaaacgg cacaggctga ggccaaaacc
     3061 ctgaattccc gaatccagcg gcgagcaaag gaagctgcca cgtcagaaaa tgccgatctg
     3121 gccacctatg ttgaaaatga tgtcggatta gaccgctata accgcctggg catcattttc
     3181 ctgttccacc
//
LOCUS       DAOQDO010000496         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23562_length_3188_cov_3.131503, whole genome shotgun sequence.
ACCESSION   DAOQDO010000496 DAOQDO010000000
VERSION     DAOQDO010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23562_length_3188_cov_3.131503"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..655
                     /locus_tag="PKZ53_09130"
     CDS             392..655
                     /locus_tag="PKZ53_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ40640.1"
                     /translation="MIKLDIVNRVADKTGVPKTKAEIAVEALLETMKKAMKEGERIEL
                     RGFGVFVVKPRKRGIGRNPRTGEVTDIPPGKTIRFKPGKELCQ"
     gene            1360..3072
                     /locus_tag="PKZ53_09135"
     CDS             1360..3072
                     /locus_tag="PKZ53_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNJ40641.1"
                     /translation="MRVQNRLRLAAFVAVCAFVVMGFTSLVRSTQAFTNKKQKPKPAA
                     SPTGSAPAATKKYAPGGGPATLTYSELVKLSQVDKPLSPGLQAKMDRLLKTPFISNSA
                     TAQPRFSTDPKIGKTLRVAEWNIERGLNFDMVVAALSGPEGFAKWIDLEKYPATSKEY
                     AAAIDQARRLQEADVLILNELDWGVKRTDYRNVPKDLATALNMNYAYGVEFLEIDPVQ
                     VGTEEFEGLPAADRAQLKKEIAVDKKRFLGMHGTAVLSRFPILSASVLRLPLVYDWFK
                     EEQKSVSLPEKAKREAINKVFLEKVLREIRRGGRNMLRVDLEVPELAEKTLTVLALHT
                     ENKCKPEGRQKQLQAVLETINAISNPVVLAGDMNTTLSDGAPTSIKNEITKRLGSKQF
                     WAVQGIKYATGVGLAWDVVTGGINFLKNQHDPTARNIPVVAPNPEVKFFEMLEGFRFD
                     DGYAFDFRGNPDRTVSGRKGTLANSNQRGGKGFLTTFAVERTIGPAGRYKLDWMFVKA
                     YSKSPRDGSQPYKFAPHFPQTMAEINDVTKDRISDHCPITIELPLTEPNIAAGEPTED
                     ETSE"
BASE COUNT          853 a          825 c          795 g          715 t
ORIGIN      
        1 ctgagggtag cgggcgaagg gcagagtacc cgcaagctcg gggctgagga aggtgggata
       61 aaaacatttt tgaataccct ggctgtgtcc ttactttgat ttatcccgag gctcttcatc
      121 ctttaggaca gtcccctggt acaaaagcct ggtttgacga tatcagcctg aaacggctgt
      181 gacggcaggt ctcaggtgaa cgaaaatacc ctaccaaacc caaattttcc tttttccaac
      241 ctggtttttc gctttttttt aaccgggagt tccctgcaac tacttgacag atattcggtt
      301 aacacatact cttcgggctt gcttcatccg ctcgacataa acaaataacc cacattgatc
      361 ttaaattttt cgaaatcttg atggaggaac cgtgatcaag ctcgacatcg tcaatcgtgt
      421 tgccgataaa actggcgtcc caaaaaccaa agcagaaatt gctgttgaag cactgctcga
      481 aaccatgaaa aaagccatga aagaaggcga acgcatcgaa ctgcgcggct ttggcgtgtt
      541 tgtcgtcaaa ccccgcaagc gtggcatcgg acgcaacccg cgtaccggtg aagtcaccga
      601 cattccaccc ggcaaaacca tccgcttcaa acccggcaaa gaactttgcc aataagggaa
      661 taagggtcaa cagactcggc tctcaagggc cgagcttgag tcgtgtaccc acgacgccat
      721 cctcacggat ggccagtcgt ggtggcgttg cctcacgctt cggttgacca gacccccgtg
      781 caaatcacgg gaaacgattg ggaagaattg ataggtacca gcggatgccg cctcagtccg
      841 ctgcgctacg gtgacaggtt aaacaggaat gggagtcagt tccagtgctt gtcacagcaa
      901 accttcccga atctggtcga gaggaagaca gattcgaccg acggtcacag tcgcgctcga
      961 tatgcattac tctccgcaag gagatggtga gggttttttc cctcacaaga gcgagtggag
     1021 ggagttggct ccttccattt gctgttcaag gttgattcaa agctggagca gcatttcctc
     1081 ccggtcctga agggccgggt ctccacacca aagacaggat gaattttgaa ttatgaacca
     1141 gtccaaccgg tgtgattcaa cctgaatcat ttttcataat tcttcattct tcatttggtt
     1201 tccaccccac ccagggcctg agtgtttgag tggtttcagc cggttcgagc ccgaagccgc
     1261 cggtttttgg aaattgctct tgcactcaag ccctcttttt ttcttcaatt caccaactcc
     1321 ctcgtttcaa cattccaacc aggtcagatt caattttcta tgcgcgttca aaatcggctg
     1381 cgactggcag cttttgtggc agtatgtgcc tttgtcgtga tggggttcac cagtcttgtg
     1441 cggtcaaccc aggcattcac aaataaaaag cagaaaccca aaccagcagc ttccccaacc
     1501 gggtcggctc cggcagccac caaaaagtat gcaccgggag gtgggcctgc gacattgacc
     1561 tattcagaac tggtcaaatt gagtcaggtt gataaaccct tatcgccagg attacaggcc
     1621 aagatggatc ggcttctcaa aacgccattt atctcaaata gtgccacggc ccaacccagg
     1681 ttttcgactg atcccaaaat cggcaaaacc ctgcgggtgg cagagtggaa tatcgaacgt
     1741 ggcctcaatt ttgacatggt ggtggctgct ctctctggac ccgaaggctt tgccaaatgg
     1801 attgaccttg aaaaataccc ggccaccagc aaggaatacg cagcagcaat tgatcaggcc
     1861 agacggttgc aggaagccga cgtgctgatc ctcaatgaac tggactgggg tgtcaaacgc
     1921 accgactatc gcaatgtccc caaagatctg gcgacagccc tcaatatgaa ttatgcctac
     1981 ggggttgaat ttttggaaat tgacccggtt caggtgggaa ctgaagaatt tgaagggttg
     2041 ccggcggctg accgcgctca actcaagaag gaaatcgcag ttgataaaaa acgcttcctt
     2101 ggcatgcatg gcacggcggt tttgagccgc tttccaatcc tcagtgcttc ggtgctacga
     2161 ctgccgctgg tctatgactg gtttaaagaa gaacaaaagt cggtttccct gcccgaaaaa
     2221 gccaaacgcg aggcaatcaa taaagtcttt cttgaaaagg tcttgcgtga aatccggcgc
     2281 ggcggacgca atatgttgcg agtggacctg gaagttcctg aactggcaga gaaaaccctg
     2341 accgtcctcg cactccatac cgaaaacaag tgcaaacccg aaggacgtca gaaacaactg
     2401 caagccgtgc ttgaaaccat caacgcaatt tctaacccgg tagtcctggc cggagatatg
     2461 aacaccacgt tgagtgatgg cgcgcccacc tcgatcaaaa acgaaattac caaacgcctg
     2521 gggagcaaac aattttgggc cgttcaaggc atcaaatatg ccacgggggt cggcctggcg
     2581 tgggatgtgg tcaccggcgg gatcaatttc ctgaaaaatc agcacgaccc aactgcccgg
     2641 aacattccag tcgtggcccc caacccggag gtcaaattct ttgaaatgct cgaaggcttc
     2701 cgctttgacg atggctatgc ctttgatttc cgaggcaatc cagaccgcac ggtgagtggc
     2761 cgtaaaggca cactcgccaa ctcaaaccag cgagggggaa aaggatttct caccacattt
     2821 gccgtcgaac gcacgattgg accagccggg cggtataaac tcgactggat gtttgtgaag
     2881 gcgtactcaa aatcaccccg cgatggcagc cagccctata aattcgcacc ccatttcccg
     2941 caaaccatgg ctgaaatcaa cgatgtcacc aaagatcgca tttcagacca ctgccccatt
     3001 accattgaac taccactcac cgaacccaat attgcggctg gtgaaccaac tgaagatgaa
     3061 accagtgaat gaagaatgaa gaatagaatt gagaatgaag aatgaagaat gaagaattgg
     3121 gaattgagaa tgaagaagtg agaatgaaga atgaagaagt gcctggtgct tggtgcttgg
     3181 tgcttagg
//
LOCUS       DAOQDO010000497         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23563_length_3188_cov_3.059368, whole genome shotgun sequence.
ACCESSION   DAOQDO010000497 DAOQDO010000000
VERSION     DAOQDO010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23563_length_3188_cov_3.059368"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKZ53_09140"
     CDS             complement(<1..381)
                     /locus_tag="PKZ53_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ40642.1"
                     /translation="MSTPEVKISSSEQSSPRQYQLLIDGKFVDAMSGRTFESLNPSTG
                     EVLAHVAEGEAEDINLAVTAARRAFESGPWARMTPHQRGKILYKAAQIITDRAKELAE
                     LETLDNGKTLRETTYVDIPQTIENF"
     gene            617..1435
                     /locus_tag="PKZ53_09145"
     CDS             617..1435
                     /locus_tag="PKZ53_09145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ40643.1"
                     /translation="MSDQRTQAYLYDLYVAPLWRERFDQLFIEEVGIPTQGQILVLEC
                     GTGGLALEVAAALKDTGTVVASDQDEEILELARAKALATKVENIRFVSSGEIQAEFTP
                     HTFDLTLGDASLLPPTQIQPMLNNLYRPLTTDGTAVLYAITRGSFDEFFSIYWEALFE
                     CGLAQQLQPQLEQVLNLYPTPEEIEAWAVAAGWKNVQLVLENEEFTFDTGEDFLSSPL
                     ISSYQIERWLHIVPTAAEIIQVRQTIATIIDRDRGGYPFDITIKAALVTGKRTT"
     gene            1539..2426
                     /locus_tag="PKZ53_09150"
     CDS             1539..2426
                     /locus_tag="PKZ53_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40644.1"
                     /translation="MNGRSLFFASDLWVKFFFVIFTTTLLWLPSALVAADDKPSLQQV
                     VDRHTQALGGTERLSKTKSRVARGKAELIPGRGSGLSQMSGEAVFYSKAPNKVRMDFV
                     FGGFGVKRGYNGTEAWIKPAPPASDSAMTKAFTSLLKSGGLLHEISVYDGLIEAFQRE
                     SDLKVKGTKKVNDIESYQLELKQTDVTPKLFVDTASGNQIRTAFSVLYNPAANPNSAA
                     PIGGSGGGLIGSSPMSSSGSFSSPTAFTVEIDTSDYREVNGIKLPHAYSERLPFYTIK
                     VTILKYEENVEISDVIFNQ"
     gene            2598..3008
                     /locus_tag="PKZ53_09155"
     CDS             2598..3008
                     /locus_tag="PKZ53_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40645.1"
                     /translation="MGTWLGFAVILMVIAALFWYELSFYGNLKVQQKDTPSAKARFGR
                     RTVGIVLLLIISLMTFLGLQHQASFPSPIFFLLYWGTCFLLTLILIVLALLDTHAVLK
                     QAVATYTDTAEEEKRFREFMKKHVQAESNKGNKP"
BASE COUNT          822 a          799 c          753 g          814 t
ORIGIN      
        1 aaagttttca atggtttgcg gaatatcaac ataggtcgtt tcccgcaggg ttttgccatt
       61 gtcgagcgtt tcaagctcgg ccagttcttt ggcgcggtca gtgataatct gtgcggcttt
      121 atagaggatt ttcccgcgct gatgcggagt catccgggcc caggggccac tttcaaatgc
      181 ccggcgggcg gcagtgacag ccaggttgat gtcttccgct tcaccttcag ccacgtgggc
      241 cagcacttca ccagttgagg gattcagcga ctcaaaagta cgtcctgaca tggcatccac
      301 aaatttgcca tcaatcaaga gctggtattg gcgaggtgag gattgttccg atgatgaaat
      361 tttaacttct ggggttgaca tatgaaacga actccttgaa tggctgtgtc gaggtgcaac
      421 acagttttga atagattttc agcctgaaac ccaaatgttc acggtcagaa atttgaggtt
      481 aaacactatt tatggagggg atccttgtgg tgaaattgcg actttatatg ccattcttcc
      541 agcaatgtga agtgattcac acctcaaacg aaaaaacagc caaccacttg aatcaaaccg
      601 gaaaggaatt ttttgcgtgt ctgaccaacg aactcaagcg tatctgtatg acctgtatgt
      661 agcaccgctc tggagagagc gctttgatca gttgtttatt gaagaggttg ggattccaac
      721 ccaggggcag atcctggtat tggaatgcgg taccggtgga ttggcacttg aagttgccgc
      781 cgctttaaaa gatactggga ctgtcgtcgc cagtgatcag gatgaagaga tcctggaact
      841 ggcccgggcc aaagctctcg ccaccaaagt agagaatatt cggtttgttt caagcggcga
      901 aattcaggct gaatttacgc cccacacctt tgatctgacg ttgggagacg cctcactctt
      961 accaccaacc cagatccagc caatgctgaa caatttgtat cgcccactca caactgatgg
     1021 cacagcggtc ctctatgcca tcactcgtgg aagtttcgat gagttttttt cgatctactg
     1081 ggaagccttg tttgaatgtg gtctggccca acaactgcag ccacaactgg aacaggtcct
     1141 gaatctctat ccaacaccgg aagaaatcga agcctgggca gtcgcagccg gttggaaaaa
     1201 tgtgcagtta gttctggaaa atgaggaatt tacctttgac accggagagg attttttgtc
     1261 atcaccactc atttccagct accagattga acgctggctt catatcgtgc ccacagcggc
     1321 tgaaatcatt caggtgagac agaccatcgc aacgattatt gaccgtgatc gaggtgggta
     1381 tccgtttgat atcaccatta aagccgcact ggtcactgga aaacgaacga cctaaacaaa
     1441 aacacacaac aagctcggcc ccaaacacgc acaacatcaa tttcatcaac gcctgaaacc
     1501 ctcttaatcc aacttttgac ggccccgagg aggattttat gaacgggaga agtttgtttt
     1561 ttgcctctga tctttgggtg aaattttttt tcgtcatctt taccaccaca ctgctgtggc
     1621 tgccctcggc actggtggca gccgatgaca aaccatcact tcaacaagtg gttgaccggc
     1681 atacacaggc gctgggcgga acagaacgac tcagcaaaac aaaatcgaga gtcgctcgcg
     1741 gaaaagctga gttaatccca ggacgcggct ccggattgtc ccaaatgtct ggggaggctg
     1801 tgttttattc aaaagccccc aacaaagtgc gcatggattt tgtgtttggc ggctttgggg
     1861 taaagcgcgg gtacaatggc actgaggcgt ggattaaacc agcccctccg gccagcgata
     1921 gtgccatgac caaagccttc acctcgttac tcaaatcagg tggattgctg cacgagattt
     1981 cagtctatga cggcctgatc gaagcgtttc aacgtgaaag cgatctcaaa gtcaaaggca
     2041 ccaaaaaggt caacgacatc gaatcgtatc aacttgaact gaaacaaacc gatgtgacgc
     2101 caaaactctt tgttgatacc gccagtggaa atcaaatccg tacagcattt tcagtgctct
     2161 ataacccggc ggccaacccc aactcagccg caccgattgg cggaagtggc ggtggactca
     2221 tcggatcatc gccgatgtcg tcatcaggtt cattttccag cccaaccgcc tttacggtcg
     2281 aaattgacac cagtgactat cgtgaggtca acgggattaa acttccacac gcctatagcg
     2341 agcgcctccc gttttacacg attaaagtca cgattctgaa atatgaagaa aatgtcgaaa
     2401 tcagtgatgt gatttttaat caatagacct gggctggggt cagtaagcta aacacgacgg
     2461 gcctgagact gaaattaagg gatgcgagat gaacgtactc cattttttca gtccaacgtc
     2521 ttcagcccca ggcccgtcgc ccatttcctc ccctcttgtg tcttcagctt gtattcccgt
     2581 gacacacacc aaacactatg ggtacctggc ttggtttcgc tgtgatttta atggtgattg
     2641 cggctttgtt ttggtatgaa ctttctttct atgggaacct caaagtccag cagaaagata
     2701 caccatcggc caaagcccgg ttcggacgcc gtacagtggg gattgttttg ctcttaatta
     2761 tttcactgat gacctttttg ggactgcaac atcaagcctc atttccttca ccaattttct
     2821 ttttgttgta ttggggtacc tgtttccttc tgacgctcat tttgattgtg ctcgccttac
     2881 ttgatactca cgccgtcttg aaacaggcgg ttgcgaccta taccgacacg gcggaagaag
     2941 aaaagcgatt tcgcgaattc atgaaaaaac acgttcaggc agagtcaaat aagggaaaca
     3001 agccgtagct cagagttttg aatgctgaaa aggatacggg ataaagaatc agaaaccctg
     3061 gcgtccccct gcccccgtca tcttatcatt tgtcaccctg tcatctgtct gtcacgctgc
     3121 catctcttcc aatgacccca cctgcaccct caaaacccgc tcgaacctgg ttgtggatct
     3181 atctctca
//
LOCUS       DAOQDO010000498         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23612_length_3184_cov_3.238415, whole genome shotgun sequence.
ACCESSION   DAOQDO010000498 DAOQDO010000000
VERSION     DAOQDO010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23612_length_3184_cov_3.238415"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1413)
                     /locus_tag="PKZ53_09160"
     CDS             complement(25..1413)
                     /locus_tag="PKZ53_09160"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNJ40646.1"
                     /translation="MAEQDYRIEKDSMGEMRVPATALYGAQTQRAVENFPISGLCFPR
                     AFIRALGMIKGAAAEVNLGLGLMEADMTEAIRSAAQEVADGQHDGHFPLDIFQTGSGT
                     STNMNTNEVIATLATQRLGRKVHPNDHINMGQSSNDTIPTAIHLSAYLEVAEALLPAL
                     RHLKHTLDEKAAQVDHVVTTGRTHLMDAMPIRLSQELSGWSTQIAYGIERVEAALPRL
                     AKLAQGGTAVGTGINAHPEFGARFATKLSEMTGLPFVTSPNYFESLSSQDAACELSGQ
                     LKTVAASLTKIANDLRWMNSGPQAGLAEIALPALQPGSSIMPGKVNPVIPEAVAMVCA
                     QVIGNDVTITMGAQSGNFQLNVMLPVIAYNLLQSVTLLANVSRLLADRAIAGFTVNES
                     HIGDLVDRNPIMVTALNPVIGYELGAKIAKQSYAQNRKVKEVAAEMTDLTPEHLDRLL
                     DPRQLTEGGINE"
     gene            1750..>3184
                     /locus_tag="PKZ53_09165"
     CDS             1750..>3184
                     /locus_tag="PKZ53_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ40647.1"
                     /translation="MNTCPQCGSTFSDDKKFCNRDGQRLVALIPTLAAPAPAPVSLNK
                     QEAATLVDSPQAADPMIGKVLAGRYRILSLIGKGGMGAVYRGEHLKLGRPSAIKVILP
                     ASAQDETTLTRFQREAETSSKINHPNAVSIFDYGETDDGMVFLAMELIEGQQLKSILK
                     REAPFSLERTLHIARQVGQALSAAHALNVVHRDLKPENIMLGSGDTVKVVDFGIAKSL
                     LVDPKHQAVTRTGFIVGTPQYMSPEQVMGEQLDPRSDVYSLGLVIYEMLTGALPFEGE
                     SAQAQMVNRLANPPQPMSRVRPGLNIPAAVEAAVMRALHYEREYRYPRVTDFIASLEH
                     AVHLETQNIGQRKTGAAGLDATQPAIAFQANLPAGQPVYVPPPPVAPLSGSPSGGYAS
                     SPNRTLPDTEQYPPPVLAQGMQAGLGGNQPLPQGPPGLGGYVPPGSGYGQVPQPGPGY
                     LPPPQPGPEYSTPPHAIPPYAPGGAAMA"
BASE COUNT          726 a          848 c          843 g          767 t
ORIGIN      
        1 tcttcattct tcattcttca ttcttcattc attgatacca ccttcagtca attgccgtgg
       61 gtcgagtaag cggtcgagat gttccggagt caggtctgtc atttcagcgg caacctcttt
      121 gaccttgcgg ttttgggcat acgattgctt ggcaattttg gcaccgagtt catacccaat
      181 gaccgggttg agtgccgtca ccataatcgg gttgcgatca acgagatctc cgatatggct
      241 ttcattgacg gtaaatccag caatggcccg atcagccagc agtcggctca cattggcaag
      301 caatgtcaca ctttgcaaca ggttatacgc aatcaccggc aacatgacgt ttaactggaa
      361 atttcctgac tgagcgccca tcgtgatagt gacgtcgttg ccaatcacct gagcacagac
      421 catggcaacc gcttcgggaa tgaccggatt gaccttgcca ggcataatac tgctgcctgg
      481 ctggagcgct ggcagggcaa tttcagccag cccggcttgg ggaccgctat tcatccagcg
      541 caaatcgttg gcaattttgg tgaggctggc ggcgacggtt ttgagctgac cgctcagttc
      601 acaggcggca tcctgactgc tgagcgattc aaaatagttt ggactggtaa caaacggtaa
      661 tccagtcatt tcagacagtt tcgttgcaaa tctggctcca aattcaggat gggcgttgat
      721 accagttccg acggcggttc caccctgggc aagttttgca agccgaggca gagcggcttc
      781 aacccgttcg atgccatagg caatctgggt gctccagcct gaaagttcct ggctgagtcg
      841 gatgggcatg gcgtccatca ggtgggtgcg gccagtggtg acgacgtggt caacctgggc
      901 ggctttttca tcgagtgtgt gcttgagatg acgaagtgct ggaagcagtg cttcggccac
      961 ttccagatag gcgctgaggt gaattgccgt tgggatggtg tcgttgctgc tctggcccat
     1021 attgatgtga tcattggggt ggactttgcg tccaagtcgc tgggtggcca gcgtggcgat
     1081 cacttcattg gtattcatgt tggtgctggt gccagaaccg gtctggaaga tatcaagcgg
     1141 aaagtgtcca tcgtgttgac cgtcagcgac ttcttgagcg gcgctccgaa tcgcctcggt
     1201 catgtcagct tccatcagcc caagtcccag attgacttca gcggcggcac ctttgatcat
     1261 tcccagggcg cgaataaacg cccgtggaaa gcaaagcccg ctaattggaa agttctcaac
     1321 tgcccgttgg gtttgagcgc catagagcgc agttgccgga acgcgcatct cacccatgct
     1381 gtctttttca atgcgataat cctgttcggc cacgagttga aatctccttt gtttcaaaaa
     1441 ataactgcga tttataggtt ggaaagaatg agaaaagtgc gcgccattct ggtcaagaac
     1501 cgttgaatgc gtcaatggtt ctttgtcttg acacaactca atccaaagct gatggcctgt
     1561 ctgtagaaag tattctggaa ccagaccgtc gcgatttaga tcaatatcag cactcccatg
     1621 ttcaatctgg gctggtttga actgtaaaaa tccatcactg acaaccacta tgaaaagtga
     1681 tataagatcc ttaccttctc accttgtttc ccaaaatgat ggtttttgtt cgctgttgag
     1741 gagattcaca tgaatacctg tccacaatgc ggctcgacat tttccgatga caagaagttc
     1801 tgtaatcgtg acggtcaacg tctggtggca cttatcccaa cgctggcagc acctgctccg
     1861 gcaccggttt cgctcaataa acaagaggca gccacgctgg ttgactcacc tcaagcggca
     1921 gacccaatga ttggaaaggt cttagccggg cgctatcgaa ttttatcgct gattggaaaa
     1981 ggtggaatgg gtgccgtcta tcgtggtgaa cacctcaaat tgggacggcc aagtgcgatt
     2041 aaggtgattt tgccagcatc tgcccaggat gaaaccacgc tcacccggtt tcaacgcgaa
     2101 gctgagactt ccagcaagat caaccatcca aacgccgtca gcatttttga ctatggcgaa
     2161 accgatgatg gaatggtttt tctggccatg gaattgattg aaggccagca gttaaaaagt
     2221 attctcaagc gtgaagcccc gttctcactt gagcgaactc tgcatatcgc ccgccaggtt
     2281 ggtcaggcgc tcagtgccgc gcatgccctc aatgttgtgc atcgggattt gaagccggaa
     2341 aacatcatgc ttggatctgg ggacacggtc aaagtggtgg actttgggat tgccaagtca
     2401 ttactggttg accccaaaca ccaggcggtc acacgtaccg gatttattgt cggaacgccg
     2461 caatatatgt caccagagca ggtgatgggc gagcaacttg acccgcgttc agatgtctac
     2521 agcctgggac tcgtcattta tgaaatgctg accggagcct tgccatttga aggtgaatcg
     2581 gctcaggcgc aaatggttaa tcgtctggcc aatcctccac aaccgatgag ccgggttcgt
     2641 ccaggactca acattccggc tgcggttgaa gccgccgtca tgcgggcact ccactatgaa
     2701 cgcgagtacc gctacccgcg agtcactgat ttcattgctt cgctcgaaca tgccgtccat
     2761 ctggaaaccc aaaatatcgg tcagcgaaaa acaggtgccg ccgggttaga tgccacacag
     2821 ccagctattg cttttcaagc caacctgcca gccggtcaac cggtttacgt tccaccaccg
     2881 ccggtggcgc cgttgtctgg cagtccaagt ggcgggtatg cgtcttcgcc aaatagaacg
     2941 cttccagata ctgagcaata cccgccaccc gtattggccc aggggatgca ggccggcctg
     3001 ggtggaaacc agccactgcc tcaaggacct cctggcctgg gaggctatgt gccaccaggt
     3061 tctggatatg gccaggtgcc ccaaccggga ccagggtatc ttcccccacc ccaaccggga
     3121 ccagaatatt ccacgccacc tcacgcaata ccaccctatg ctcctggtgg ggccgccatg
     3181 gccc
//
LOCUS       DAOQDO010000499         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23645_length_3182_cov_3.165974, whole genome shotgun sequence.
ACCESSION   DAOQDO010000499 DAOQDO010000000
VERSION     DAOQDO010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23645_length_3182_cov_3.165974"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1730)
                     /locus_tag="PKZ53_09170"
     CDS             complement(195..1730)
                     /locus_tag="PKZ53_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNJ40648.1"
                     /translation="MKLEVEFDEKTPVYLQIKQAIADQIQQGVLPIGARLPATRDLAH
                     QLGVNRGTVTMAYDELVDDGYLESHVGRGTFVARRLVTNTAVATVGQVTQTIPPMTWE
                     GIFADHIESPVIDSLLDLYQVSMLQDVISFSGSFPDSGSFPAQEFQRSLNTVIDQLGN
                     SVLAYGPAQGFDLLREFLAREMKDQGISVSPDQILITNGSQQALDLIARAFLSPGDTV
                     VVENPTYPGAVNAFTLAGAHLIGIPVDEHGIRTEAIEKVIEFKRPKLIYTVPSFQNPT
                     GVTLSLERRHHLLNLAWDHGIPIVEDDYGSCLSFDGAGMPILKTQDAGDHVIYVNTFS
                     KSLLPGLRLGWCAAAKPVVSRLTALKQNSDISSSTLLQAALWEFCQRGQYQAHLERVR
                     PLYRERRDRAVQILDREFPTGTTWTCPNGGLFLWVTLPETVDATELLYHSRQKGVVFS
                     RGHLFYVENPAKNTLRLSYGNVSLDQIETGLSIIGRTAQELVARSSERTPVRFRTHTV
                     PLV"
     gene            complement(2518..>3182)
                     /locus_tag="PKZ53_09175"
     CDS             complement(2518..>3182)
                     /locus_tag="PKZ53_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNJ40649.1"
                     /translation="VMPTDAPRPKSDATAGYGAKIIWYDREREDREAIARDLAEREGR
                     TLIPPYDDLQIMAGQGTAALELFEDTEHLDVLLAPVGGGGLLSGCSTVAQVKNPGIQI
                     FGIEAEAANDTFLSFQSGHRVSIPVPATIADGMRNTAPGTLTFPVLQRNIQGIVLVSD
                     QEILEAMRFLLFRMKMLVEPTGAVGVAALLAGKIPAHGKRVGVILSGGNVEPEILNRL
                     IE"
BASE COUNT          848 a          814 c          744 g          776 t
ORIGIN      
        1 ctttcaataa taaaagtgcg gtttcgtacc acctggagaa tgaagaatga agaatgtgac
       61 tggtgcttag tacttggtgc ttagtacttg gtgcttagta ttttgtgctt ataaatcaat
      121 gctttccaag aaccaagaac caagaaccaa gaaccaagaa ccaaccactt gtatgttctt
      181 catccttcac tctcttagac cagtggcaca gtgtgtgttc gaaatcgaac cggtgtccgt
      241 tcgctgctgc gagcgaccag ttcctgagcg gtgcgtccaa tgatactcag tccagtttca
      301 atctgatcaa gactgacatt tccataactc aaccgaagtg tgtttttggc cgggttctcg
      361 acgtaaaaca agtgacctcg actaaacaca accccttttt gccgtgaatg atacagtaat
      421 tctgttgcgt caacagtttc cggtaatgtt acccacaaaa ataatccgcc attggggcag
      481 gtccaggttg tccccgttgg aaattcccgg tccagaatct gaactgcccg atcccgtcgt
      541 tcgcgatata gaggccgaac ccgctccagg tgagcctgat attgcccacg ctggcaaaat
      601 tcccacagtg ctgcctgcaa cagagttgag ctggaaatat cgctgttttg cttgagtgcc
      661 gtcaggcggc tgacgacggg tttagctgcg gcgcaccatc caagccgaag gccagggagc
      721 aagctcttag aaaaagtatt gacataaatc acatggtcac ccgcatcctg cgtcttgaga
      781 attggcattc cagcaccgtc aaagcttaaa caagaaccat aatcatcttc gacaatcgga
      841 atcccatgat cccaggccaa attgagcaga tggtggcgac gttccagact caacgtcact
      901 ccggttgggt tttggaaact gggcacggtg taaatcaatt ttggccgctt gaattcaatc
      961 accttttcga tggcttcagt tcggatgcca tgctcatcaa ctggtattcc aatcaaatgg
     1021 gctccagcca gtgtaaatgc gtttacagca ccaggatacg tcgggttttc aacaacaacg
     1081 gtatcacccg gtgaaagaaa tgcccgggca attaaatcga gtgcctgttg tgacccattg
     1141 gtgatgagaa tctggtcagg tgaaactgaa atgccctgat ctttcatttc acgagctaaa
     1201 aactcccgga gcaggtcaaa cccctgggct ggaccgtagg ccagaacgct gtttccaagc
     1261 tgatcaatca ccgtattaag tgagcgctga aattcctggg ccggaaacga accgctgtca
     1321 ggaaaggaac cagaaaaaga aatcacatcc tgcaacatac tgacctgata caaatccagc
     1381 agcgaatcaa tcaccgggct ttcaatatga tcggcaaaga ttccttccca ggtcatcggt
     1441 ggaatagtct gagttacttg cccgacagtg gccaccgctg tattggtgac cagacgtcga
     1501 gccacaaacg ttccacgtcc gacgtgtgat tccaaatagc catcatccac cagttcatca
     1561 tacgccattg tcacagtgcc tcgattgaca cccaactggt gggctaaatc gcgggttgct
     1621 ggcaaccggg caccgatggg gagcactcct tgctgaatct gatcggcaat ggcttgcttg
     1681 atttgcaaat acactggcgt tttttcgtca aattcaactt cgagtttcat tgaaaactcc
     1741 caaagccgga ggtactccaa ccagatggat ccagcggggc aaatatcagt acactggaac
     1801 caggtcggtg cagcttttct atcttgtttt gtgtttttgt cgagcagttg aagagtggag
     1861 gaattggact aatccaagcc agccaatact aaagaaaaaa gccagccaat gcaagaaaaa
     1921 cttgcattgg actggctttt attttgattt tttctcaaat ggactcctcc attggctaaa
     1981 atcaggcgca tctaaccttt attcaaccag tccaatttgc tggattggac tggcagatgc
     2041 cgtggaggct ggttcctggt tcctggttct tggttcttgg ttcttcgaaa gtattgattc
     2101 ctaagcacca agtactaagc actaagcacc aagtactaag cactaagtac taagcactaa
     2161 gcaccagtct cgctagccaa tgaaaaaacc atagacacga aaggtttgga ccgcaatttc
     2221 gaggttttca aagaaggaca ccatcaaatg acgcaccata gggttgcctc ctctcgggaa
     2281 ctgtcagttg ggggatagaa gaaacacgcg gcaggaacag acagtttccc ttaagcaacc
     2341 cttgtgccgt ttccccaaaa ccagaaactt tatttagtcc ttaaatataa aggacttacg
     2401 ggcagcaaac cgatcatact tctttggctg gaaccgacct gaatccaaat ttcagataaa
     2461 tattgtctga tttatgacag ttgaacagtc atctcaaacc agatcgtctg gccacggcta
     2521 ctcaatcagg cgattgagta tttcaggttc aacattgcct ccagacaaaa tcaccccgac
     2581 gcgcttgccg tgagccggta tttttcctgc cagcagtgca gccacgccaa ctgccccggt
     2641 tggttcgacc agcattttca ttcggaaaag caaaaagcgc atggcctcca ggatttcctg
     2701 gtctgaaacc agcacaatgc cctggatgtt gcgttgcagg acaggaaagg tcaatgttcc
     2761 aggagccgtg ttgcgcatac catcagcaat cgttgcgggc acgggaatcg aaacccggtg
     2821 acctgattgg aaagagagaa atgtatcgtt tgcggcttcg gcttcaattc cgaaaatttg
     2881 aatcccagga ttttttacct gggcgaccgt gctacaccca ctgagcagcc cgccgccacc
     2941 aactggtgcc agaaggacat ccagatgttc agtatcttca aagagttcca gagcagccgt
     3001 tccctgcccg gccataatct gaagatcatc atatggagga atgagcgtcc ggccttcgcg
     3061 ttcagccagg tcccgggcga tggcctcacg gtcttcacgc tcgcggtcat accagataat
     3121 tttggcgcca tatccagccg tggcatcact tttgggacgc ggagcatcgg tcggcatcac
     3181 aa
//
LOCUS       DAOQDO010000500         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23672_length_3180_cov_2.732480, whole genome shotgun sequence.
ACCESSION   DAOQDO010000500 DAOQDO010000000
VERSION     DAOQDO010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23672_length_3180_cov_2.732480"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKZ53_09180"
     CDS             complement(<1..1007)
                     /locus_tag="PKZ53_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40650.1"
                     /translation="MVLSDNFKALAKLYYNPIRAMNDIIDQGSLLFAMTVAAVATLVF
                     YLALAIPMLTVVGNLWFVGSSLLPSTQQVAPAQTSSSTSSTADEAEEESPPIQPGFTP
                     TRMLYLLYAFLSSPMSILSWVAALTLLYVPASIIVGSWFIPTARIGILFQQHYGSLLA
                     CTCMAWAAAFLPGSVLLLVGPVGKLFLAPFVVSLGTLYFAMLMVCALRVNLGSEYQGA
                     ILTVLVSWLSLLLAPIFSSCLSYVMLPWIAFYLYGYIRGEASFVSTAFSGQQSFRRYL
                     EASTINPRDAEAHYQLGLIHQKRRQYGDAIDRFQRSVEIDPNEIDAHYQLGRIAREQK
                     R"
     gene            complement(1109..1780)
                     /locus_tag="PKZ53_09185"
     CDS             complement(1109..1780)
                     /locus_tag="PKZ53_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40651.1"
                     /translation="MPHSVNGIGTGLVKASRERKIDGYVQFDAIEALILFYIPIIPYK
                     AIHVLSIWQDEHGGEKYDSIQLRPSGRFIAKAMFNGWGNVLLIFAGITLLIAGWAFIS
                     MSRPMNIKDWMCLGGLTTAFIGGFFCKSIWYLMNETDERIKDIIGPHDLGTSDPVDWS
                     DEIAQSVIKHLMAEANGQTLVGIANQALAEQNRAKAMLCVRLAMRGGQNPVAQKLFDQ
                     LMASV"
     gene            complement(1933..>3180)
                     /locus_tag="PKZ53_09190"
     CDS             complement(1933..>3180)
                     /locus_tag="PKZ53_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ40652.1"
                     /translation="LVSVLGDSDSDVRKSATYALWNIGDERAIQPLTGELNDSTRAVR
                     EAAAEALQALGWRAQTTEEKALLAVAVHDWDLASQLGKDAFDILVATLKDHDKEIQQG
                     AIEALGKIGDPRALEPLFDMLKHWEPDVRRAALRALSIIHDPRAVDAILVLLDDKESE
                     VRKMAAKALGEFKQPQSVVPLLARLKDKASGVRQTAAEALGKLGDPQATGHLVAGLVD
                     EDSEVREASAWALSALRWVPEDEIQFIRCSIALRNWDELVNVGAAAIPFLIEALNDKH
                     NYVRVAAAYALGQIKDPAAGDQLLNLLKHRNAEVRGVVAEALGSLGDVRAVRPLLRLT
                     EDSDIASRVIGALRQLLEQSAPQFSIDELRAVIRLDNVQQVRLETDRQLETYKSWNTK
                     QLVNCGYIRQLAQEELSRRGLTI"
BASE COUNT          795 a          835 c          817 g          733 t
ORIGIN      
        1 aagcgttttt gttcgcgggc gatccggcca agctggtaat gggcatcaat ttcgttcggg
       61 tcaatctcga cagagcgttg aaaccgatca atcgcatcac cgtactgacg ccgtttttga
      121 tgaatcaacc cgagttgata atgggcctcg gcatcccgag gattaatggt cgaagcttcc
      181 agatagcggc gaaagctttg ctgtccacta aatgcggttg aaacaaaact ggcttcaccg
      241 cgaatgtagc catacagata aaatgcgatc cagggaagca tcacatatga aaggcagctt
      301 gagaaaatcg gtgccagcaa caatgaaagc caggacacca gaaccgtcag aatcgcaccc
      361 tgatattcag atccaagatt gacccgcagg gcacagacca tcaacatcgc aaaatagagc
      421 gtgccaaggc tgaccacaaa gggcgccaaa aacagcttcc caaccggtcc gacgaggagc
      481 aagaccgatc cggggagaaa ggctgccgcc caggccatac aggtacaggc cagcagcgac
      541 ccataatgtt gttgaaataa aatcccgatc cgggccgtcg ggataaacca cgatccaaca
      601 atgatcgagg ctggcacata gagcagggtc aaggctgcca cccaggacaa aatcgacatt
      661 ggtgaactta aaaaggcata aagcaagtac agcatccggg ttggcgtaaa tccaggctgg
      721 atgggcggcg actcttcttc agcttcatcg gctgtgctac tggtggaact gctggtttga
      781 gccggtgcca cctgttgggt tgatggaagc agcgatgacc cgacaaacca caggttcccc
      841 accacggtca acatcggaat tgccagcgcc aggtaaaaca ccagggtcgc caccgctgcc
      901 accgtcattg caaagagcag gctgccctgg tcaataatgt cattcatggc gcggatcggg
      961 ttgtaataga gcttcgccaa agctttgaaa ttgtcagaaa gtaccataag agaatcagaa
     1021 agaagcgcat tggggattca ggttttggga tgaataattt ggggttgccg cgccccaccc
     1081 aacggaagac cagccagaaa ctggtttttc acacagacgc catcagttgg tcaaacagct
     1141 tttgagcgac cgggttttgg ccaccccgca tggcgagccg aacacagagc atggctttgg
     1201 cccgattttg ctcagccagt gcctgattgg caattccaac cagggtttgg ccattggctt
     1261 cagccatcaa atgcttgata acggattggg cgatttcatc agaccagtca accgggtctg
     1321 aggtaccgag atcatgaggt ccgatgatgt ccttaatgcg ttcatctgtt tcattcatca
     1381 aataccagat gcttttgcaa aaaaatccac ctatgaaggc agttgtcaac ccgcctaaac
     1441 acatccagtc cttaatgttc atggggcggc tcatcgagat aaaagcccac cctgcaatca
     1501 acaatgtgat accggcaaaa attaagagaa catttcccca tccattgaac attgctttcg
     1561 caatgaatcg cccggacggt ctgagttgga tagagtcata tttttctcca ccgtgttcat
     1621 cctgccagat tgacagcaca tggatagctt tatagggaat gattggaatg taaaacagga
     1681 tcaaggcttc tatggcatca aattgaacat acccatcaat cttgcgttct cttgaggctt
     1741 tgacgagtcc tgttccaatc ccgttcacgc tgtgtggcat aaccagagcg ctcctttcct
     1801 tatagaaatc aagattttcc agtcagaaat tgagtgactg gtaaaagagt gaacgagtga
     1861 aaagcggata gtggtttggt tttggactat tcgctccgca ctccttactt tttcttgcga
     1921 cctcgctatt gttcaaatag tgagtcctct gcgagacaat tcttcctggg cgagttgccg
     1981 gatgtatccg caattgacca gttgtttggt attccagctt ttataagttt ccaactggcg
     2041 atcagtttcg agccggactt gctggacgtt atccagccga atcaccgccc gtaactcgtc
     2101 aattgaaaac tgaggtgcac tttgttctaa aagctgtcgc agggcaccga tcacccgact
     2161 ggcaatatca gaatcttcag tcaggcggag caacggacgg acggcccgaa catcgcccag
     2221 cgagccaagg gcttcggcca cgacaccacg gacttcagca ttgcgatgct ttaataaatt
     2281 gagaagctga tcaccggctg ccgggtcttt aatctgacca agcgcatagg ccgcagccac
     2341 cctgacgtaa ttatgtttgt cattgagcgc ttcaatcaaa aatgggatgg ctgctgcccc
     2401 aacattgact aactcatccc agttgcgcag ggcaattgag caccgaataa actggatttc
     2461 atcctcaggt acccagcgca gggccgagag agcccaggct gaggcttcac gaacttcact
     2521 atcttcatca accagcccgg cgaccagatg accagttgcc tgtgggtcgc caagtttgcc
     2581 aagcgcttcg gcggctgttt gccgaacacc gcttgctttg tctttgagcc tggccagcag
     2641 ggggaccact gattgtggtt gcttaaactc accaagtgct ttggcggcca tctttcgaac
     2701 ttcgctttct ttgtcgtcca ataagaccag gatggcatca actgcccggg ggtcatggat
     2761 gatgctcagg gcccggagtg cggcccgtct gacgtcaggt tcccagtgct tgagcatgtc
     2821 aaagagcggt tccagggctc gggggtcgcc gatttttcca agggcttcga tagcgccctg
     2881 ttgaatttcc ttatcgtggt ctttcagagt tgccaccaga atatcaaagg catctttccc
     2941 aagttgggac gccagatccc agtcgtgaac ggctacggcc agaagtgctt tctcttcagt
     3001 cgtctgggct cgccagccaa gtgcctgaag ggcttcggcg gcagcttcac gaactgcccg
     3061 ggttgagtcg ttgagttcgc cagtcagggg ctggatcgcc cgttcgtcgc cgatgttcca
     3121 tagcgcgtag gttgcgcttt tgcggacatc gctgtcactg tcgcccagta ccgaaaccag
//
LOCUS       DAOQDO010000501         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23686_length_3179_cov_2.843150, whole genome shotgun sequence.
ACCESSION   DAOQDO010000501 DAOQDO010000000
VERSION     DAOQDO010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23686_length_3179_cov_2.843150"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..2059
                     /locus_tag="PKZ53_09195"
     CDS             371..2059
                     /locus_tag="PKZ53_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40653.1"
                     /translation="MRVKQALVSGLLVLVCLSSVAISGQEKPATPPPDTKPVSLAVFK
                     NGLGFVVRQGKINLVEDGWGTLHTVPNATFGSLWLAPGDPGVILEEVIASQGDITKPK
                     SVQSLAELLQANVGKPATVFINDKVSYSGVILPTPDEPETSSPPGTRDNRAGYSYLPP
                     QVPSELFLMKVNDSVMAFRHSQVQFIALGSAPVTKLEVKEKSKLLKFKAKGATSAASI
                     TMGYLQKGMGWTPSYLVSLKDDKTAQVTMQAVLINDMEDLLDTDVFFVVGFPNFAVSD
                     IPSPMTLQQTLAEFMASLQSGGSSSSYAYGRLDNLRTNRIMAQQANAYDADDTGLDYS
                     TTVNELEGNSEEDLFLYNRQHVNLKKGERGTYNVFSSEVPYEHVYQWDVSNILQIDDD
                     GNINRSAAPKAEDQVWHSLRLKNVSNVPWTTAPAMTISGNKPIAQDVLNYTPKSASTL
                     LKLTVATDVRVERQELETERVQNAKKRYGYDYDAVTVTGTLKVKNFKPTNIKLSIKKT
                     VRGEVITAAELGKSEKLAVAIQAVNPTSRITWDIPLKPGEEKTIQYTYKVFVRP"
     gene            complement(2251..2703)
                     /locus_tag="PKZ53_09200"
     CDS             complement(2251..2703)
                     /locus_tag="PKZ53_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40654.1"
                     /translation="MNLPQLLEFKRNDLINEALGILNQARLSHYDDAGYSQRRLYLIR
                     LYDLMTQCIEQNDMVPMLNYIKALAEERFTTGFDLSEVQMAFNVMEEVIWKHILSDLP
                     AEKYPEALGMISTVLGTGKDYLANMYVSMASQHKATSIDLSDLYQKAR"
     gene            2945..>3179
                     /locus_tag="PKZ53_09205"
     CDS             2945..>3179
                     /locus_tag="PKZ53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNJ40655.1"
                     /translation="MVLLSNRYRVLQQLGQGAYGETFLVEDTHLPSNRRCVLKQLKPT
                     TDRALQEELIERFKEEAAILERLGEQSPHGQIPK"
BASE COUNT          855 a          769 c          786 g          769 t
ORIGIN      
        1 agacagacaa agaacggcgc acgtgcagaa tacctcgtga gaaatattgg taaatgaatt
       61 ggatgggata gatagaaaat cagtgttgga actgagcccc aaattatgaa ggtgttacgc
      121 ctttcggtca acctgacgca gtgcgttgtg taccagaaaa ttccagcgac agaaacaaat
      181 gccccccgtc accatccatc tgagaggggg ttccctcgcc aggttcaata aaatgaatgg
      241 gtttttggag gaaaatccag atttcttgga acgaatttcc attttcagcg agcaatgtga
      301 tgtcccacgc cttagaatca attcagcagt tcaagaagtt cacaaactat ccacaaaaaa
      361 ggggaaagtt atgcgcgtaa aacaagctct ggtttcaggg ttgttggttc tggtgtgtct
      421 ttcatcagtt gcgatttcag gacaggaaaa accggcgaca ccgccgccgg acaccaagcc
      481 ggtgtcgctg gctgtcttta aaaacgggct gggatttgtg gttcgacagg gaaaaatcaa
      541 tctggttgaa gatgggtggg gcaccctgca taccgtgccg aatgccacct ttggttcgct
      601 ctggctggcg cctggtgacc caggcgtgat cctcgaagaa gtgattgcgt cacagggaga
      661 catcacaaag ccaaaatcgg ttcagtcact ggctgaactt ctgcaggcta atgtcggcaa
      721 acctgcaaca gtctttatca atgacaaggt ttcttatagc ggagttatcc ttccaactcc
      781 ggacgagcca gaaacctcgt cacctccagg cacgagagat aacagagctg gctacagcta
      841 tttgcctcca caggtcccat cggagctttt tctgatgaaa gtcaatgata gtgtgatggc
      901 atttcgtcat tcacaggtgc agtttattgc attagggtcc gctccagtga caaaactgga
      961 ggtcaaggaa aagtcgaagt tgttgaaatt taaagccaaa ggggcaacca gcgccgcctc
     1021 gattacgatg gggtatttgc aaaaaggcat gggctggacg ccatcatacc tggtttcgct
     1081 caaagacgac aaaaccgccc aggtcacgat gcaggcggtg ttgatcaatg acatggaaga
     1141 cttgctggat acagatgtct ttttcgtcgt tggcttccca aattttgccg tgtcggatat
     1201 tccgtcgcca atgacgttgc agcaaacact ggctgaattt atggcaagtt tgcaaagtgg
     1261 agggagttct tcttcctatg cctatggacg actggacaat cttcgaacca atcgaatcat
     1321 ggcgcagcag gccaatgcct atgatgctga cgatactgga cttgactaca gcacgacggt
     1381 caatgaactg gaagggaatt cagaagaaga cctttttctc tacaaccgtc agcacgtcaa
     1441 tctgaaaaaa ggtgagcgtg gcacctacaa tgttttttca agcgaagtcc cgtatgaaca
     1501 cgtctatcaa tgggatgtgt ccaatattct acaaattgat gatgatggga atattaaccg
     1561 ttcagccgct ccgaaagcgg aagatcaggt gtggcactcg cttcggttaa aaaacgtgtc
     1621 gaacgtgccc tggacgacag cgccagccat gaccatttca ggcaacaaac cgattgccca
     1681 ggatgtgctc aactacacgc cgaaaagcgc cagcacgttg ttgaaattga cggtggcgac
     1741 tgatgtgcgg gtcgaacgtc aggaactgga gaccgaacga gtacagaatg cgaaaaaacg
     1801 ctatggctat gactatgatg ccgtgaccgt cactggtacg ctcaaagtga aaaacttcaa
     1861 acccacaaac atcaaacttt caatcaagaa aacagttcgc ggcgaagtca tcacggcggc
     1921 tgaattgggc aaatctgaaa aactggcggt tgccattcag gccgtcaacc caacctcacg
     1981 catcacgtgg gacattccgc tcaaacccgg cgaagaaaag acaattcagt acacttacaa
     2041 agtctttgtc cgaccataaa gaccaggggc tgaaaacttc gggttgaaga cttcgggctc
     2101 agggctgaag aaaacgggct cagggtattg aacccgcttt tcttcagccc aatgtcttca
     2161 gccccaagcc ctcagcccat tttcttcagc ccgttttctt cagccctgag cccaatgtct
     2221 tcagccccaa tcccggcgtc ttccgcccca ttacctggct ttctgataca aatctgacag
     2281 atctatcgag gtggctttgt gctggctggc catcgaaaca tacatattgg ccagataatc
     2341 ttttccggtt ccgagcactg tactgatcat gccaagggct tccgggtatt tttccgctgg
     2401 caggtccgac agaatgtgtt tccagatcac ttcttccatg acattgaagg ccatctgaac
     2461 ttcagaaagg tcaaacccgg tggtaaagcg ttcctccgcc aatgccttga tgtaattcag
     2521 cattggaacc atgtcatttt gttcaataca ttgagtcatc aagtcataca atcggatcag
     2581 atacagtcgc cgttgactgt acccggcgtc gtcatagtgt gagagccggg cctggttcag
     2641 gattccgagt gcttcgttaa tcagatcgtt gcgtttaaat tcaagcagtt ggggtaaatt
     2701 catagtggtt gttgcacctc cgctaaatgt ggggcaattt cctcaaaacc aacctgcttg
     2761 agtaatgagc aatcagcatt ccagattttg ctgtcagaga tggtaggcaa gcgaggtgtt
     2821 gggcaggtag aatgcactca gggtttaggg ggcaaggttt cgaatttatg tcctttatgt
     2881 cctttatgtc ctttatgtcc tttaaaccct gacccctgac ccctgacccc cgaaccctaa
     2941 atctatggta ctgctcagca atcgctaccg tgtgttgcaa caacttggac agggtgccta
     3001 tggcgagacg tttctggtcg aagatacgca tcttccgtcc aatcgccgat gtgtattaaa
     3061 acaattgaaa ccgacgactg atcgagcact gcaagaggaa ttgattgagc ggtttaaaga
     3121 agaagctgca atcctggaac ggcttggaga gcagtcgcca cacggccaaa ttcccaaac
//
LOCUS       DAOQDO010000502         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23769_length_3173_cov_2.810455, whole genome shotgun sequence.
ACCESSION   DAOQDO010000502 DAOQDO010000000
VERSION     DAOQDO010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23769_length_3173_cov_2.810455"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3112)
                     /locus_tag="PKZ53_09210"
     CDS             complement(<1..3112)
                     /locus_tag="PKZ53_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ40656.1"
                     /translation="MPTPVNLRSLSEPSRLTDVELAAQYFSVSLGALVPIGQVLARCV
                     EGLRRAYQVSCCRLLIWEDEGQSARVAAEAIDEQARVSFATQPTAELPLLTQQGNIFE
                     TRQFPLLREFRTRFHPIWHPGLTESDVAPSRYELPWGKLGAQQSLIVFPLQTADRLLG
                     LVILETMNLRTWTLAEVRFIQVATNHLVSSIEAAQNFAELETDVHRLNVINEISRSLN
                     ASFSIDEIFRLLVNLTRTLVDFDLASLLLADERSGQLKLHSQALHEHSWFTFGQWLSA
                     GFALCGRALAKRQPLVIPNLQTETFFPVRADWIERGFASCTIIPLLARGKTLGVLMFL
                     SQKPDVFGGREVDILRQVGDQAAAGIQRVQTFDESEKRSHAAKQVARREELINRIGMA
                     IGSQLHPDMVLQHAVDALGTSLDADCCYIAQCLATDMETFARYEFRARAWKSVIEMPI
                     PVLDNPTLQRVLSSGKPIVMDDECTYLKAEAGLPLAPPMSAFLKRLGVKLALYSAIYV
                     GEKAVGFLGLHQYEQPRGWSEDDISLVRAVTRQLAVALGNAQLFTESERNRRELSNLF
                     EMSRVLSSYTDLRELFELVTQRVAVSLGAEMCVLALLNKRTGYLSAQSPGYNVPAEML
                     SRLRFMYQACDLEHRQGSGETPIVSLTDVLREDECEALASGLPHPDMPYFSNQAQYDS
                     RLPLTFLKQWKIRNLMLCPLRMKGEVIGYIYLANGPAGFTTEDLQLLEIFAGQAAEAI
                     VNAQLFEHVQAQAQREATLNRILTGLRESLDPDSIMENVVHQLGKTLGVDRCTISLFT
                     AREKFWPGIQHEYCAPGIASIKNNAIINQTRIKVVGSFRAPLIINDFNAHSLDRLVTD
                     HWDLLHHAGIRSLVMIPIFQAQELIGSIGLHHCQESHTWQQSEIDLARTVAAQLNVAI
                     QNARLFRRVVASQQQWQASFNAMTDGVALLDADGRVVQANLSLARLCGLDTPEAMVGH
                     SAWDLIIPRNGFQDDHPISEVLQTKVSVQTEFVDLDGHVIRETIDPIQDENDQLMGMV
                     MVVSDVT"
BASE COUNT          798 a          911 c          777 g          687 t
ORIGIN      
        1 gggtgacatc gctgaccacc atcaccattc ccattaactg gtcgttttca tcctggattg
       61 ggtcaatggt ttcgcgaata acgtgtccgt ccagatcaac aaactcggtt tgaacactca
      121 ccttagtctg taaaacctca gaaatcggat gatcatcctg gaatccattc ctcggaatga
      181 tcaagtccca ggcactgtga ccaaccatgg cctccggtgt gtcaaggcca cacagccgtg
      241 ccagcgaaag attggcctga acaacccgcc catcggcatc cagcaaggca acaccgtcgg
      301 tcatcgcgtt aaacgacgcc tgccattgtt gctgacttgc caccacccgt cggaacagac
      361 gggcgttctg gatagccacg tttaactgag ccgccactgt ccgcgccaga tcaatttcac
      421 tctgctgcca ggtatgtgat tcctgacaat ggtgcaagcc aattgagcca atcagctcct
      481 gggcttgaaa aatcggaatc atcaccagtg atcgaatccc ggcatggtgc aataaatccc
      541 agtgatcagt caccagccga tccaacgaat gcgcattgaa atcattgatg atcagcgggg
      601 ctcgaaacga accaactact ttgatccggg tctggttgat gatggcattg tttttgattg
      661 aagcaatgcc cggtgcgcaa tattcgtgtt gaattccagg ccagaacttt tcgcgtgcgg
      721 taaacaacga gatggtacag cgatctacgc ccagcgtctt gcccaactga tggactacgt
      781 tttccataat cgagtcgggg tcgagtgact cacggagccc agtcagaatc cgattgaggg
      841 tggcttcgcg ttgcgcctga gcctggacgt gttcaaacaa ctgggcattc acaatcgctt
      901 cggcggcctg cccggcaaaa atttcgagca gttggagatc ttccgtagta aaccccgccg
      961 gcccgttggc caggtaaatg tatccaatca cctcaccttt catccgaagc gggcacaaca
     1021 tcaggttacg aattttccac tgcttgagaa aagtgagcgg caagcgcgaa tcgtactggg
     1081 cctggttact gaaatagggc atatccgggt gagggagccc ggaggccagc gcctcgcact
     1141 catcttcgcg caacacgtcg gtcagactga caattggcgt ttcccctgaa ccttgccgat
     1201 gctccagatc acaggcctga tacataaacc gtaaccggga cagcatttca gccggaacat
     1261 tgtatccagg agattgggcc gacaaatacc cggttcgttt attgagcaaa gccagcacgc
     1321 acatttcggc ccccaggctc accgccaccc gctgggtcac cagttcaaat aattcacgca
     1381 aatcagtgta ggaagacaac acccgtgaca tttcaaacag gtttgataat tctcgccggt
     1441 tgcgttcgct ttcagtaaac aactgggcat tgccaagcgc caccgccagt tgacgggtca
     1501 cggcccgaac caggctgatg tcgtcttcac tccacccacg aggttgctca tactggtgca
     1561 atcccaaaaa tccgacggct ttttcaccaa cataaatggc actatagagc gcgagcttga
     1621 cccccagccg ttttaaaaac gctgacatcg gtggcgccag cggcaacccg gcctcggctt
     1681 tgagataggt acattcatca tccatcacaa tcggcttgcc gcttgacagc acccgctgga
     1741 gcgtcgggtt gtcgagcacc ggaatcggca tctcgatgac gcttttccag gcccgcgccc
     1801 gaaactcata tcgggcaaac gtttccatat cagtggccag acactgggca atgtaacaac
     1861 aatcggcatc caggctcgtc ccaagcgcat ccaccgcgtg ctgcaacacc atgtcgggat
     1921 gaagttgact tccaatcgcc atcccaattc gattgatgag ttcttcacga cgggccacct
     1981 gtttggcagc atgtgatcgt ttttctgatt catcaaaggt ctgcacgcgc tggattccag
     2041 ccgcggcctg atctcctacc tggcgtaaaa tatcaacttc ccgcccgcca aagacgtccg
     2101 gtttttggct taaaaacatc aacacgccca gggttttccc tcgggccagc agcggaataa
     2161 tcgtgcatga ggcaaatcca cgctcaatcc aatctgcccg aactggaaaa aatgtctccg
     2221 tttgtaagtt tggaataacc agcggttgcc gcttggccag tgcccggcca cacagggcaa
     2281 acccggctga cagccattgc ccaaacgtaa accagctatg ttcgtgcagc gcctgggaat
     2341 gcagctttaa ctgacccgac cgttcatcag ccagcaacag actcgccagg tcaaaatcca
     2401 ccagggttcg cgtgagattg acaaggagcc gaaagatttc atcaatacta aagctggcat
     2461 tgagtgaccg gctgatttca ttgatcacat tgagccggtg gacatcggtt tcgagctcgg
     2521 caaagttctg agccgcttca atcgaagaca ccagatgatt ggtggccacc tgtataaacc
     2581 gcacctcagc cagggtccag gtccggagat tcatcgtctc aaggataacc aatcccagca
     2641 agcggtctgc cgtctgaagc ggaaacacaa tcagactttg ctgtgctccc aactttcccc
     2701 acggcaactc atatcgggat ggggcaacat cgctttccgt cagccctgga tgccagattg
     2761 ggtgaaacct ggttcgaaac tcacgcaaca acggaaactg ccgggtttca aagatatttc
     2821 cctgttgagt caaaagtggc aactcagccg tgggctgggt cgcaaatgat actcttgcct
     2881 gctcgtcaat agcctcagcc gcgactctgg cagactgccc ttcatcttcc caaatcaata
     2941 agcgacaaca acttacctgg tatgcccgtc gcaacccttc gacacaccgg gccagtacct
     3001 gcccaattgg aaccagcgcc cctaacgaca ccgaaaagta ctgggctgcc agctcaacat
     3061 cggtcaagcg cgaaggctca gagagggatc tcaaattgac aggtgtcggc atagtggtgg
     3121 aatgaagaat tgagaatgaa gaattgagaa tgaagaattg agaatgaaga att
//
LOCUS       DAOQDO010000503         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23788_length_3171_cov_3.627086, whole genome shotgun sequence.
ACCESSION   DAOQDO010000503 DAOQDO010000000
VERSION     DAOQDO010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23788_length_3171_cov_3.627086"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKZ53_09215"
     CDS             complement(<1..707)
                     /locus_tag="PKZ53_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ40657.1"
                     /translation="MSATMPAASKSEKTFVKGGSFLIQTTAPADVFTPEDFTEEQLMI
                     GKTTNDFIQNEVLPLLPQLEKQSQTQNFDLTVSLIRKAGELGLLAGDIPAKYEGLELD
                     KVCSMLMAEGVGVSGGFSVSIGGHTGIGTWPITFFGTPEQKAKYLPKLGTGELLAAYA
                     LSEPGSGSDALGARTRAVLSEDGKHYILNGSKMWITNAGFADIFIVFCKIDGDKFSCF
                     IVPRDSEGLSTGKEEDK"
     gene            complement(873..1499)
                     /locus_tag="PKZ53_09220"
     CDS             complement(873..1499)
                     /locus_tag="PKZ53_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40658.1"
                     /translation="MIVPSFRKLILGSTLTALCLGATWMLPYSTPVQAASLSHPTGQN
                     DAGKVPEYISDMAGIKFKKPAPDWQVLDSGPELVQRILCLPPGETLSPFSPRLVILVY
                     PASAVVGGMEFRRQQVSTVTAEGKVKLTQLKFENSSMAGKDAVLYSYDLEFQTPIHTV
                     EYGFVNQGNFYVVQIAAVASEWEKPEFTAMMDKALKSVGFVKIPNLNK"
     gene            complement(1741..2922)
                     /locus_tag="PKZ53_09225"
     CDS             complement(1741..2922)
                     /locus_tag="PKZ53_09225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNJ40659.1"
                     /translation="MKEAVIVSAVRTAVGKALKGTLAYTRPDDFGAVCLKAAIDRAPG
                     LDPKEIEDVIIGCAMPEAEQGMNVARIASLRAGLPNSVSAVTVNRFCSSGLQAIAYAA
                     ERVMVGSSEVILAGGIESMSLIPMGGHKISPNPWLVDNYPDAYLSMGLVAERLAKNYE
                     ISRAQQDEFSCLSHQRASTAIAEGKFKDEIVPVPVKHTSINAKGKKVVTETIFDTDEG
                     PRADTSVAALAKLRPAFHAAGSVTAGNSSQMSDGAAACIVMSSEKAQALGLKPLARFV
                     AYATAGVPPEDMGIGPVYAIPKALKLAGLTLDDIKVIDLNEAFASQALSVMKELNLDP
                     ERVNVNGGAIALGHPLGCTGAKLTASVINEVKRRGGGYGMVTMCVGGGMGAAGIFEVF
                     A"
BASE COUNT          752 a          817 c          798 g          804 t
ORIGIN      
        1 atcttgtctt cttctttccc ggttgaaagt ccttcagagt cacgtgggac gataaagcac
       61 gaaaacttgt cgccatcaat tttacagaag acgataaaga tatcggcaaa cccggcgttg
      121 gtgatccaca ttttgctgcc gttcaaaatg tagtgtttgc catcttcgct cagcacggca
      181 cgggtgcggg cacccagcgc atcagaaccg ctgcctggtt cagacagggc gtaggcagcg
      241 agcagttcac ccgtgcccag tttgggcagg tacttggcct tttgttctgg cgtaccaaag
      301 aaggtaatcg gccaggtgcc gatacccgta tggccgccga tggaaacaga aaagcctccg
      361 gagacgccga cgccttcggc catcagcatc gagcacactt tgtcgagttc cagtccttca
      421 tacttggccg gaatatcacc tgccagcaag ccaagctctc cggctttgcg gatgagtgat
      481 accgtcaggt caaagttctg ggtttgggat tgtttttcaa gctgtggcag caagggcagg
      541 acttcgtttt gaatgaagtc attggtggtc ttgccgatca tcagttgttc ttcggtaaaa
      601 tcttctgggg tgaacacatc ggctggagca gtggtttgga tgaggaagct cccgcctttg
      661 acgaatgttt tctctgactt ggatgcggct ggcatggtag cagacataag tgaacgtcct
      721 ttcttgttgt acttcaataa attatgagaa cgggggcggt ttatgaatga atcatcattc
      781 aatttccgtg tccactgctc ccgaagacgc tcactcgcgc tggatcactt cggaagcaaa
      841 accttggtga agggggaaga cagtggagct ggttacttgt ttaagtttgg aattttgaca
      901 aaacccacgc ttttcagcgc tttgtccatc atggcggtaa actcaggttt ttcccactcg
      961 ctggcgacgg ctgcgatctg gacgacataa aaattccctt gattgacaaa cccatattca
     1021 acggtatgaa tcggggtttg aaattcaagg tcatagctat agagcaccgc gtctttacct
     1081 gccatcgagg aattttcaaa tttcaattgc gtcagtttga ctttcccttc ggcagtgact
     1141 gtactgactt gttgtcggcg aaattccatc cctccgacca cggcactggc cggatagacc
     1201 aaaatcacca gacgcgggga aaaagggctc aaggtttcac ctggaggcag acacaagatc
     1261 cgctgcacca gttctgggcc gctgtcgagc acctgccagt caggtgctgg ctttttaaat
     1321 ttgattccgg ccatgtcgct gatgtattca ggtactttcc ctgcatcatt ctggccagtc
     1381 gggtgagaca ggcttgccgc ctgcactggt gtcgaatacg gaagcatcca ggttgctccc
     1441 aggcagagtg ccgtcagggt tgatccgagt atcagttttc gaaaggaagg gacaatcatc
     1501 gttcggtcac ctttcacgtg aaaatgagtt caacaagaaa cttcaggttt tggaattttg
     1561 atactgagac cggaaggaac ttccatctgg ttctcagttt cgttgcttga tgctcaccct
     1621 accatccttg aacctgaaat caaaccccaa accctaaact gatggaccct ggacagcatt
     1681 ggtgcctggt acctggtgct tggaagaatg aatttccagg caccagtcac cacgcactga
     1741 ttacgcaaag acctcgaaaa tgcctgccgc ccccatgccg ccaccgacac acatggtgac
     1801 catgccgtac ccgccgccac gccgtttgac ttcattgatg accgaagccg tgagtttggc
     1861 gccagtgcag cccaacgggt gaccaagggc aatggcgccg ccgttgacgt tgacgcgctc
     1921 tggatcaagg ttgagttcct tcatcactga cagcgcctgt gaggcaaagg cttcgttcaa
     1981 atcaatgact ttgatatcgt cgagtgtcag cccggcaagt ttgagggctt tcggaatcgc
     2041 atagactggg ccgatgccca tatcttcggg tggcacgcca gccgtggcgt aggccacaaa
     2101 acgggccaga ggtttgagtc cgagtgcctg tgccttctct gatgacatta caatgcaggc
     2161 ggctgccccg tcactcattt gtgacgaatt tcccgcagtg acactgcctg ccgcatggaa
     2221 ggctggccgc agttttgcca gcgcggcaac tgaggtatca gcgcgcgggc cttcatcggt
     2281 atcaaagatg gtttcggtga cgactttttt ccctttggcg ttgatggatg tatgtttgac
     2341 cggaaccggg acaatttcgt ctttaaactt tccttcagca atggcggtac ttgcccgctg
     2401 atggctcaaa cacgaaaatt catcctgctg agcgcgtgaa atttcataat tcttagccag
     2461 ccgttcagcg accagaccca ttgacaggta ggcatccgga tagttgtcaa ccagccatgg
     2521 gttgggcgag attttatgac cgcccattgg aatcaacgac atgctttcga ttccaccagc
     2581 cagaataacc tctgagcttc caaccatgac ccgttccgca gcataggcaa tcgcctgtaa
     2641 ccccgaagaa caaaaccggt tgacagtgac cgcgctgacg ctgtttggca gcccagcgcg
     2701 aagtgaggcg atccgggcca cgttcatgcc ttgctctgct tcgggcatcg cacaaccgat
     2761 gatgacgtct tcaatctctt ttggatccag accgggcgca cggtcaatcg ccgctttcaa
     2821 acataccgcg ccaaaatcat ccgggcgggt ataggccagt gtgcctttga gtgcttttcc
     2881 aacagccgtt cgaacggcag atacaattac agcttctttc atgacagctc ctctttggaa
     2941 tgaagaagaa atcagaatga agaattgaga atgaagaatg aagaaaataa acagatggtt
     3001 aaatattgag agttcgaaga acgtctttta cataaagaat tgagagttgg aagagcctta
     3061 aagaatcaat gaacgccgga agcacttaga ttcccaatct caattcttca ttctcaaatt
     3121 cttcattctt cattcttcat tcttcattct tcattcttca ttcttcattc t
//
LOCUS       DAOQDO010000504         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23808_length_3170_cov_3.445586, whole genome shotgun sequence.
ACCESSION   DAOQDO010000504 DAOQDO010000000
VERSION     DAOQDO010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23808_length_3170_cov_3.445586"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3170
                     /locus_tag="PKZ53_09230"
     CDS             <1..>3170
                     /locus_tag="PKZ53_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ40660.1"
                     /translation="RFPMATDYKVTVPAGTKSALGMALAAETSWKFSTPPPTVKSTSP
                     SGQAEPLTPLLFVAFDQKINPEAVLKTIRVTADGKSVPVKLATSEQIQADAVVANYAK
                     YTEPGYWLAFQSVEPLPKNVGVTVTVQAGAPSAEGPRTTPADQSFNFRTYGPLILTQS
                     RCGWDESRCRPSDQWNLSFSNSLDIRDFRKEQITITPTLKDADISVSGSEITVTGAKP
                     GSTTYTVTVDRSLKDTYGQVLGKPVTVTFKVGTDDPSFLVSGNTLTTLEPAGAKQLPV
                     YTTNVPELRVRMFQVTPADWHAFLAFRRDWYQRQQKERITPPGKLISTQRIPVRGKAD
                     ELTETRIDLTPALSNGLGHVIVLVKPLGIKSTEYTFDAEPRWVQSTRLAVDALVDNTD
                     LVAWANSLADGKPVENAEVVLFPENLTAKTGTDGLARLALKSEPAQKPNFLLVRKGVD
                     TAILPETDGWWQENGDWHRNATTDGLRWFVFDDRKLYKPGETINVKGWIRRLGTAKDG
                     DIGPATGIGETVTYAFFDSQGNEFAKGTTTINAYGGFSLTGDIPKAVNMGYSRVELSV
                     TASPTNGPTDTSTTHTFQIEEFRRPEYEVTVNATDGPHLIKGFAETTVNASYYSGGGL
                     GNAETNWRVSAKGGSYSPPNWDEFTFGTWQPWWRDFSSRFQPEQVETFTAKTDFAGQH
                     RLRIDFDSATPPRPYVITAEASVQDVNRQTISGATSFIVHPSALYVGVRVNRTFVNPG
                     EVFAVDTIVTDIDGKVQTGRQVMLQAVRMDWVFEGGAWKQKEIEVQKTTLTSTERPMS
                     WSFSPSEGGSYKLTARVMDDLERFNQSELTLWVSGGKQPPSNDLEQQTVDLIPNQKTY
                     QPGETAEILVQSPFVPAEGLMTVERTGILSTERFTMTGPAMTLKVPIKESYLPNIHVQ
                     VDLTGAAERMGTDGKVDPKLPKRPAYAKGTLNLRIPPMTRKLSVKPVPRETALQPGSE
                     TAVEVEIRDAAGKPVSNSECALVVVDESVLALTGYKMQDPLELFYQDREEGVQPFYFR
                     PQVVLKSANQLDDSQPLDGRDRLELS"
BASE COUNT          771 a          891 c          847 g          661 t
ORIGIN      
        1 gcgttttcca atggcgaccg attacaaagt cacggttccc gccggcacaa aatcggcact
       61 gggaatggcc ctggccgctg aaacttcgtg gaagttttca acgcctccgc cgacggtgaa
      121 aagcacctcg ccctctggcc aggccgaacc gttgacgccg ctcctgtttg tggcctttga
      181 tcagaaaatc aaccctgaag ccgttctcaa aaccattcgt gtcacggctg acggcaagtc
      241 cgttccagtc aaactggcga cctccgaaca aatccaggct gatgccgtgg ttgccaacta
      301 tgccaaatat accgaacctg gatactggct ggcctttcaa tcggtcgaac cgctcccgaa
      361 aaatgtcgga gttacagtca ctgtgcaggc tggcgcgccg tccgccgaag ggccacgaac
      421 cacgcccgcc gatcaatcct tcaattttcg gacgtatggc ccgctgattt tgactcagag
      481 tcggtgcggc tgggatgaat ctcgctgccg cccaagtgac cagtggaatc tgtcgtttag
      541 taactcgctc gacattcgcg attttcgcaa agaacaaatc acgatcacgc caaccctcaa
      601 agatgccgat atcagcgtga gcggcagtga aatcaccgtc acgggtgcca agcctggcag
      661 cacgacctac accgtgacgg ttgaccgcag tttgaaagat acctatggcc aggtgctcgg
      721 aaaaccagtc accgtgactt tcaaagttgg cacggatgac ccgagctttt tggtttcggg
      781 caataccttg acgacgctgg aaccggctgg tgccaaacag cttccggtgt atacaaccaa
      841 tgttccagaa cttcgggttc ggatgtttca ggttaccccg gcagactggc atgcgtttct
      901 tgcgttccgc cgggactggt atcaacgcca gcaaaaggaa cgtatcacac cgcccggcaa
      961 gctgatttca acccaaagga ttccggttcg cggcaaagcc gatgaattga ccgaaacacg
     1021 gattgatttg acacccgcgc tttccaatgg cctggggcac gtcatcgtgc tcgtcaaacc
     1081 gctgggaatt aaatccactg aatacacttt tgacgccgag cctcgctggg tacagtcaac
     1141 ccggctggcg gttgatgcgc tggtggataa caccgacctg gttgcctggg ccaattcgct
     1201 cgcggatgga aaaccggtcg aaaacgctga agtcgtgctg tttccggaaa acctgactgc
     1261 caaaactggg accgatggat tggccagact ggcgctcaag tcggaaccgg cccagaaacc
     1321 gaatttcctg ctggttcgca aaggtgttga caccgccatc ctgccggaaa ccgatggctg
     1381 gtggcaggag aatggtgact ggcatcgcaa cgccacgaca gatggcctgc gctggtttgt
     1441 gtttgatgat cgaaaactct acaaacccgg cgaaaccatc aatgtcaaag gctggattcg
     1501 gcggcttgga accgccaaag atggcgatat tggcccggcg accggcatcg gtgaaaccgt
     1561 gacctatgcg ttttttgatt cgcaaggcaa tgaatttgcc aaagggacca caaccatcaa
     1621 tgcctatggc gggttttcgt tgacgggcga tattcccaaa gccgtcaata tgggctattc
     1681 ccgagtggaa ctttccgtca cggccagccc gactaatggt ccaaccgaca cctccaccac
     1741 gcataccttt caaatcgaag aattccgccg gcctgaatac gaagtcaccg tcaacgccac
     1801 cgacggcccg cacctgatca aaggttttgc tgaaaccacg gtcaatgcca gttactactc
     1861 cggcggcggg ttgggcaatg ctgagaccaa ctggcgcgtg tcggccaaag gtggctcgta
     1921 cagcccgccc aactgggatg aattcacatt tgggacctgg caaccctggt ggcgtgattt
     1981 ttcgagccgc ttccaacccg aacaggttga aacgtttacc gccaaaaccg actttgctgg
     2041 tcagcatcgg ctgcgaattg attttgattc ggcgacgcct ccgcgaccct atgtgatcac
     2101 tgccgaagcc tctgtccagg atgtcaaccg gcagacgatt tctggcgcca cgagctttat
     2161 cgttcatcca tcggcactct atgtcggcgt cagagtcaac cgcacctttg tcaatccagg
     2221 cgaggtcttc gccgttgata ccatcgtcac cgacattgat ggcaaggtcc aaacgggtcg
     2281 gcaggtgatg ctccaggcgg tgcggatgga ctgggttttt gaagggggtg cctggaaaca
     2341 aaaggaaatt gaagtccaga aaaccacgct gacctcaaca gaacggccta tgtcgtggtc
     2401 gttttctccg agcgaaggcg ggtcatacaa actgaccgcc agggtgatgg acgacctcga
     2461 acgctttaac cagtcggaac tgacgctctg ggtttcgggt ggtaagcaac caccatccaa
     2521 tgatctcgaa cagcaaaccg tggatttgat tccaaaccag aaaacctacc agccgggcga
     2581 aacggctgaa attctggttc aatcgccgtt tgtacctgct gaaggtctga tgacagtgga
     2641 gcgaaccggg attctctcca ccgaacgctt caccatgact ggaccggcga tgacgttgaa
     2701 agttccaatc aaggaaagct acctgccgaa tatccacgtt caggttgacc tgactggcgc
     2761 ggctgaacga atgggcaccg atggaaaagt tgatccgaaa ttgccaaaac gcccagccta
     2821 tgccaaaggt acgctcaacc tgagaattcc accgatgacc cgaaagctct cggtgaaacc
     2881 ggttccgcga gaaacggcgc tccaacccgg aagtgaaacc gctgtcgaag tcgaaatccg
     2941 cgatgcggct ggaaaaccag tctccaacag cgaatgtgcc ctggtcgtgg ttgacgaatc
     3001 agtgctggcg ctgacgggtt acaaaatgca ggatccactg gagttgtttt accaggatcg
     3061 tgaagaaggt gtgcaacctt tttacttccg gccacaagtg gtactcaaat ccgcaaatca
     3121 attggacgac agccagccac ttgatggaag agatagattg gaattatcaa
//
LOCUS       DAOQDO010000505         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23835_length_3168_cov_2.679409, whole genome shotgun sequence.
ACCESSION   DAOQDO010000505 DAOQDO010000000
VERSION     DAOQDO010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23835_length_3168_cov_2.679409"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKZ53_09235"
     CDS             complement(<1..235)
                     /locus_tag="PKZ53_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40661.1"
                     /translation="MKDYVHRFRAAIRLWRFRLHRANVAGLRIFLKLIPEEGQRVFAL
                     TIFVGAICGIAAVAFHLAIHFAEKTLPYKIYTFI"
     gene            complement(270..2846)
                     /locus_tag="PKZ53_09240"
     CDS             complement(270..2846)
                     /locus_tag="PKZ53_09240"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNJ40662.1"
                     /translation="MFSSVPSYFEARPKMAHLAAALVLLILVTGMFWRVVFLGETMVD
                     AATLQNQLPWGGSGPTYLGHSYNRRDITDTYITREYFLVDSYKRGEWPMWSPYNLGGH
                     PMYADGVFRMFSPFNVLYWVFDVPRAYDLTCLLQLFLTGLFTYLFFINIRLGPFPALF
                     GAMTFMFASLTVFHVTSSGWLGGLMWLPLIFLSLDRAIERGSHWAAVGAGVCLAMQFY
                     CGYMPNQIYYLGAIVTYYGFQALFNPKFKASGSVNQWLARVPVAKLLVISLVVGMALS
                     AAQWMPIFELLGYSNRKIVPTELEPNWLPPWYLITALLPHVFGEAYDPKFAVLFVGIG
                     VSHAHTVYFGLSGVLLGGFGGYVWWVRRKVSAVLTEDRLRLGYFLFLLVFSTFVMCAV
                     PLYVHITQYIPVLQTIRVTNRVSVLFTFSTAAVVAYGLHFLLQLEGDVLDGFTRWIKR
                     LGLFYLSMMGLVITLVSTVAQTGWYQGMMFNYNSLRRMIRAPLAISENFDLTNVDYFP
                     GTLIPLVFFGLVFALFLTWRRGRLSQPVFCAMLLAVAVVGLGWNASQLNESYPRSLVY
                     HPSAATTFLQSDPDVFRVVVAPANFSTKGQVSQFRKIVAPPNTLVPYRIQTISGKDQL
                     FPRWYREMASAVQSQNYLSHVVFDPLQSSWYDLWNVKYLMTNDDTPFVPEHYVERFAG
                     EGVRIYENPKAKPRAFFVPRAIGMPDTPLARKKDTTPDEVVKLIKSPGFDPLQTAYVQ
                     GDGAQGEILYRPAPDDFVKMVRYQNNTIQIAAKSASGGVLVLSDTYYPGWNVYVDGVK
                     HPLWRADHALRAVKLTAGEHQVEFRFEPVRLKLGLVLSGIALVALTLWGSASLLLNRR
                     SQ"
BASE COUNT          916 a          851 c          760 g          641 t
ORIGIN      
        1 tgatgaaggt ataaatctta taaggtaatg tcttttcggc aaaatgaata gccagatgaa
       61 aagccactgc cgcaatgcca cagatagcac caacaaaaat cgtcaaggca aagactcgtt
      121 gaccttcttc agggatgagt tttagaaaga ttcgtaaccc ggcaacattt gcccggtgca
      181 ggcgaaacct ccacagccga atagcagctc gaaagcgatg tacataatct ttcacaagcc
      241 ctcctttcta cggaaaaccg gcctcacatt cattgtgagc gccgattcaa tagtaagctt
      301 gcagatcccc acaaagtaag tgcaaccaat gcaattccac tcagcacaag ccccaatttg
      361 agacgtaccg gctcaaaccg gaactcaacc tgatgttcac cagccgtcaa tttgaccgct
      421 cgcaacgcat ggtcagcccg ccagaggggg tgtttcacgc cgtcaacata cacattccag
      481 cccggatagt aggtatcaga cagtaccagc accccacctg aagcactttt cgccgcaatc
      541 tgaatggtgt tgttttgata ccgcaccatt ttgacaaaat cgtcgggtgc tggacgatac
      601 aaaatttcgc cttgggcacc atctccttga acataggctg tttgcagcgg gtcaaaccct
      661 ggcgacttga tcaatttcac aacctcatcc ggggttgtat ctttcttgcg cgcaagcggc
      721 gtgtcaggca tcccaatcgc acggggaaca aaaaatgcgc ggggtttggc ttttgggttt
      781 tcgtaaatgc gcaccccttc cccagcgaag cgttcaacgt agtgttccgg cacaaacggc
      841 gtgtcgtcgt tggtcatcag gtatttcaca ttccacaggt cataccagga tgactggagc
      901 gggtcaaaaa ccacatggct caggtaattc tgactctgga cggcagaagc catttcccga
      961 taccagcgtg gaaacaactg atctttgccg ctaatggttt gaatgcgata gggcaccagg
     1021 gtatttggtg gagcgacaat ttttcggaac tggctgacct gtcccttggt gctgaaattc
     1081 gccggggcga cgacaacccg aaacacgtcc gggtcagatt gcaaaaatgt ggtcgcggca
     1141 ctcgggtgat agaccagtga ccgggggtag ctttcattca actgactggc attccacccc
     1201 agacccacta ccgcgaccgc cagtagcatt gcgcaaaaca ctggctggct cagacgtcca
     1261 cgcctccagg tcagaaacag cgcaaatacc agtccaaaaa acacaagcgg gatcaacgtt
     1321 cctgggaaat agtctacatt ggtgaggtca aagttttccg aaatggccag tggtgcccgg
     1381 atcattcgcc gcaaactgtt gtagttaaac atcattccct gataccagcc ggtttgggca
     1441 acggttgaaa ccagggtgat gaccagcccc atcatcgaga gatagaacag tcccaggcgt
     1501 ttgatccagc gagtgaatcc atccaggaca tcaccttcga gttgcagcag aaagtgcaat
     1561 ccgtaggcca ccacggcggc tgtcgaaaag gtaaacagga cactcacccg attcgtcacc
     1621 cggatggtct gcaaaaccgg aatgtattgg gtgatatgga catagagggg caccgcacac
     1681 atcacaaacg ttgagaaaac cagcaaaaac agaaaatacc ccagtcgtag ccggtcttca
     1741 gtcagtacag ccgagacttt ccgccgcacc caccacacat agccgccgaa cccccccagt
     1801 aacacaccac tcaacccaaa atacaccgtg tgggcatggg aaacaccgat tccgacaaac
     1861 aacacggcaa atttggggtc ataggcttca ccaaacacat gcgggagtaa cgccgtgatg
     1921 agataccacg ggggcaacca gtttggctcc agctccgttg gaacaatctt ccgatttgaa
     1981 tatccaagca gttcaaaaat cggcatccat tgggccgccg aaagagccat gcccaccacc
     2041 agcgaaatca ccaggagttt cgcaaccgga acccttgcca gccattgatt gactgaacca
     2101 gaagctttga atttcggatt gaagagtgcc tgaaacccat aataagtcac gatggcaccg
     2161 aggtaataaa tttgattcgg catgtatccg cagtaaaact gcatggccag acagaccccg
     2221 gcaccaactg ccgcccagtg tgaaccgcgt tcaatcgccc gatccaggct caaaaaaatc
     2281 agcggcagcc acatcaaccc gccaagccac cccgaactgg tcacgtgaaa gaccgtcagg
     2341 ctggcaaaca taaacgtcat tgccccgaaa agtgctggaa acggaccaag tcggatattg
     2401 ataaaaaaca aataggtaaa taagcctgtc aaaaagagtt gcagcaggca ggtcaaatca
     2461 taggcgcgcg gaacatcaaa tacccagtac aacacattga atggcgaaaa catgcgaaac
     2521 acaccatccg catacatcgg atgcccaccc aggttgtacg ggctccacat tggccattcg
     2581 ccccgcttgt aagaatccac cagaaaatat tcgcgggtga tgtaggtatc ggtaatgtcg
     2641 cgtcggttgt aagaatgtcc aagataagtt gggccactcc caccccacgg aagctggttt
     2701 tggagcgttg ccgcatcaac catggtttct ccgagaaaaa ccactcgcca aaacattcca
     2761 gtcaccagga tcaaaagcac cagcgccgca gccagatggg ccattttcgg acgcgcttcg
     2821 aaataagatg gtacagaaga aaacatagaa aggcagtcaa tagtcagtag tcagtagtca
     2881 gtagtcagtt atgcaaatga agagttggac agtgcgtggg aaagtgggcg actgtatcgc
     2941 gttgagccac ttcaaacaag gggcttagga aggatagaca gtcagtacca cctgcgtgag
     3001 cgggtggggg ataaattaga acctgctaaa ccgggttgca caatggatac caaagggtta
     3061 agaaccaggg tttcattttc accttatgcc aatcactgaa ataacaggct aactgattga
     3121 aaaaagtgtc ttttcctgaa ccctgaaccc tgaaccctac tggtctta
//
LOCUS       DAOQDO010000506         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23985_length_3156_cov_2.760077, whole genome shotgun sequence.
ACCESSION   DAOQDO010000506 DAOQDO010000000
VERSION     DAOQDO010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23985_length_3156_cov_2.760077"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKZ53_09245"
     CDS             complement(<1..243)
                     /locus_tag="PKZ53_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNJ40663.1"
                     /translation="MKRELDRLEDAKQSVTGKAPYQVSATYTLPHHMVTTAFELEGYR
                     IVRNLGVVRGITVRSRSVIGSIAAGLQTIVGGNITVY"
     gene            complement(349..1677)
                     /locus_tag="PKZ53_09250"
     CDS             complement(349..1677)
                     /locus_tag="PKZ53_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNJ40664.1"
                     /translation="MKFPSKLPQKPFELLERFARQYVQRYATRLKLHPAAGRRIPKEL
                     RPQNITALANRFPRGLKDPGFAYLLEQIDDGPMYSTRYVQLFFQGEAAFQSAVEAIQN
                     ATHEILVQSYILRDDETGKGLLELLEAAADRGVKVKVLADAIGSWTTKQRFWDQMQAR
                     GIEARLFHPLLTHLKYFNIRDHRKIMVVDRTIAFTGGMNIGDEYGSLLRKRLKIRTLR
                     DTHARSEGELAQAMAAVFEESWCRAGGSPLPELPFFPEITAVGPRVLVMDTRFGRGQM
                     EAISTLVAIAAAARNRLWITNAYFAPRPFGIDILTETARRGIDVRLLLPGVSDVPLVR
                     HAGHGCFRELLENGVRIFEYQKAVLHAKTIVADDFISVVGSSNLDSRSLFFNAECSWA
                     ILDRTVAQAMVLAFENDLNFAREIKLDDWRARTLLHKVGDKLAHQLSPFL"
     gene            2031..>3156
                     /locus_tag="PKZ53_09255"
     CDS             2031..>3156
                     /locus_tag="PKZ53_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNJ40665.1"
                     /translation="MRDELRTPLKTITGYAHVLIEDASHLGEVSVLPDLRRILETGQT
                     LLTRVDAMVEPSMVDNSSDQFDLESFARSIRQDVRAPLNAILGATELLLEDVGRREVK
                     ALIPDLQKIQLAAQNLLLLINSMINLSHFADDEPAISDPGGAAPFFEVLDSEPRKVPV
                     TILIAAGNVLIRSLLLRFLTRQGDRAITADRGQRVIEVLDQHPVDLLLLDLNLAEMNG
                     FEVLQAIRRYESLQTLPVIVIAALTDIDLIAQSLQLGAEDYLPLPLNRVLLQARINAI
                     LERKRLRDRVVKHMQELAETVNQLKHSQAQAMEANRAKSTFLANMSHELRTPLNAVIG
                     FSQMMARDLTLTSHHRETAGIIMRSGEHLLSLINDVLSISK"
BASE COUNT          759 a          814 c          821 g          762 t
ORIGIN      
        1 atagaccgtg atgttccctc cgacaatggt ctggaggccg gcggcaatgc taccaatgac
       61 tgatcgcgaa cgaacggtaa tcccacgcac cacacccaga ttgcgcacaa tccggtagcc
      121 ttcgagttca aaagcggtgg tcaccatatg atgtggcagt gtgtaggtcg ccgaaacctg
      181 atagggtgct tttcctgtga cggattgttt ggcgtcttcc agtcggtcca attcgcgttt
      241 catggctctc atttcctttt ttggagtgct acggcttgac gtagctttta tatccggttt
      301 tgactcgtaa tgaaagcggc gtcaagccgc agcactccac actccagctt agaggaacgg
      361 gctgagctgg tgcgccagtt tatcgccgac tttgtggagc agcgtgcgtg ccctccagtc
      421 gtcaagtttg atttcgcgag caaagttcag gtcattttca aaggccagta ccatggcctg
      481 agcgacagtc cgatccagaa tggcccagct acattcagca ttgaaaaaca gcgacctcga
      541 atccagattt gatgacccga ccacggaaat aaaatcatcc gccacaatcg ttttggcgtg
      601 gaggacggct ttctggtatt caaaaatccg aacgccgttt tcgagtagct cgcggaaaca
      661 gccgtggcca gcgtgccgaa ccagcgggac atcgctcaca ccgggtaata acagccggac
      721 gtctatgccc cggcgcgccg tttcagtcaa aatatcaatc ccaaaaggac gcggcgcaaa
      781 gtaggcattg gtgatccaga gtcggtttcg agccgccgcc gcaatcgcca ccagcgtaga
      841 aatggcctcc atctgccccc gtccaaaccg ggtatccatc accagcactc gcgggcccac
      901 ggctgtaatc tcagggaaaa acggcagttc agggagtggg cttccgccag ctcggcacca
      961 gctttcttca aatacggcgg ccatggcctg agccagctca ccttcgctcc gggcgtgtgt
     1021 gtcgcgcaag gtacgaattt tcaggcgttt gcgcagcaat gatccatatt catcgccaat
     1081 gttcatgccg ccggtaaagg caatcgttcg atcaaccacc ataattttgc ggtggtcgcg
     1141 gatattgaaa tattttaaat gggtgagcag cggatgaaag agccgggctt cgatgccacg
     1201 cgcctgcatt tgatcccaaa accgctgttt ggtcgtccac gaaccaatgg catccgccag
     1261 aactttgact ttgactccac ggtcagccgc ggcttcgagt aattccagca agccttttcc
     1321 tgtttcatca tcccgcagga tataagactg gaccaggatt tcatgcgttg cgttttgaat
     1381 ggcttcgacg gctgactgaa atgcggcttc accttgaaaa aacaactgga catagcgggt
     1441 cgaatacatc ggcccgtcat caatttgttc gagcaaatag gcgaatccag gatctttgag
     1501 ccctctggga aaccggttgg ccagggccgt gatattttga ggccgtaact cttttggaat
     1561 ccgccggcca gccgccgggt ggagtttcaa ccgggtggca taccgttgga catactggcg
     1621 ggcaaaccgt tcgagcaatt caaatggttt ttgaggaagt ttggatggga acttcatgaa
     1681 gttgagtggt cgggaggctt cagattaaaa ccagaatata tagaaattca gataaagaaa
     1741 ccacggaaga cacggaatac acagaaaata aatccaggtt cttcaatagg ttctgagact
     1801 tcccgtcata aaaacccagg aaatatttat ctgaaaaact ataattgtaa tgagtataat
     1861 accaatcttc ctggggggaa tgtacctttt cgaaaaaatt gatttttttc tggaaaaagg
     1921 gtaatcaatg aatgactatc gaaaaaaagc cccagattca ctactatcgc ttgcaactca
     1981 ccctcactcc tgtgtcagga cagtctttgt tggctgcttc tttcacgact atgcgcgatg
     2041 aactccggac gccactcaag acaattactg ggtatgccca cgtcctcatt gaggatgcca
     2101 gtcacctggg cgaagtgtcg gttttgcctg accttcgacg gattctggaa accgggcaaa
     2161 ctcttttgac cagggttgat gcgatggttg agccctcaat ggttgacaat tcctccgatc
     2221 agtttgacct tgaatctttt gcgcgatcta tccggcagga tgtacgagcc ccgttaaatg
     2281 cgattcttgg ggcaaccgaa ttgttgctgg aggatgttgg gcggcgtgag gtcaaagcgc
     2341 tgatccctga tttgcaaaaa atacaactgg cggcccagaa cctccttttg ctgatcaata
     2401 gcatgatcaa tttgtcacat tttgccgacg atgaacctgc gatatcggat ccgggtgggg
     2461 ccgcgccgtt ttttgaggtg cttgattctg aacccagaaa agttcccgtc accattttga
     2521 ttgctgccgg caatgtgctg attcggtcgt tactgctgcg gtttctcact cgtcaggggg
     2581 atcgggcaat cacggctgac cgtggccaga gggtgattga agtactcgac cagcatccgg
     2641 ttgacttgct cctgctggac ctcaacctgg cggaaatgaa tggatttgaa gtcttacagg
     2701 ccatccggcg ctacgagtca ctgcaaacgc ttccagtgat tgtgattgct gccttgaccg
     2761 acattgactt gatcgcacag agtcttcagc ttggggctga agattacctg ccgctgccgc
     2821 tcaaccgggt gttgctgcaa gcccggatca acgccattct ggagcgaaaa cgcctgcggg
     2881 accgggtggt gaagcatatg caggaactgg ccgaaaccgt caaccagtta aaacattcac
     2941 aggcgcaggc gatggaagcc aatcgggcaa aaagcacgtt tctggccaat atgagccacg
     3001 aattgcggac cccgctcaat gccgtgatcg ggttttccca aatgatggcc cgtgatttaa
     3061 ccctcacctc tcaccatcgg gaaactgccg gcatcattat gcggagtggc gagcatttgt
     3121 tgagtttgat caacgatgtg ctttcgattt ccaaaa
//
LOCUS       DAOQDO010000507         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_23989_length_3156_cov_2.441793, whole genome shotgun sequence.
ACCESSION   DAOQDO010000507 DAOQDO010000000
VERSION     DAOQDO010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23989_length_3156_cov_2.441793"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1510)
                     /locus_tag="PKZ53_09260"
                     /pseudo
     CDS             complement(1..1510)
                     /locus_tag="PKZ53_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591616.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
     gene            complement(1679..2542)
                     /gene="cpdA"
                     /locus_tag="PKZ53_09265"
     CDS             complement(1679..2542)
                     /gene="cpdA"
                     /locus_tag="PKZ53_09265"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF008359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HNJ40666.1"
                     /translation="MPEPTPVVSAAKHLSSVMLFQFTDLHLFPSRDGKLLGINTFDSL
                     QQVFELALEQEDIPDAILATGDLAQHATAETYHLLQSCFETFCRQLRKTESAPLIPVY
                     WLAGNHDEVEMMASHPVTEPINPANAFKIKEWQVLMLNSNVPGEVHGRLTVEELNRLD
                     QALAQNPDRFTLVCLHHNPVPTTDDWMGEIGLHEADHFFEVIDRHSNVKGIVWGHIHQ
                     EFVGTRKGVLMLATPSTCFQFKPNSVEFALDHINPGFRWIHLLANGDIRTGLHRVKDF
                     KQKAERKSKGY"
     gene            2845..>3156
                     /locus_tag="PKZ53_09270"
     CDS             2845..>3156
                     /locus_tag="PKZ53_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNJ40667.1"
                     /translation="MAYENILVDIQGPVGIVVLNRPQSLNAIDKATIVELLGAFETLR
                     DTDAVRAIVFTGAGRAFSSGGDIKEMIRSQGATSDEVFREHIGDINRTISLMGEIPKP
                     VI"
BASE COUNT          757 a          824 c          746 g          829 t
ORIGIN      
        1 ggtaatcgtc ttcacaactt tgatgcgggc ataatatttg tcattggcct ccacaatcgt
       61 ccacggagcc cagcgggtgc tggttttttt gatcatatca accactgcat tttcatagag
      121 gtgccatttg gcccgattgc gccagtcttc ttcggtcaac ttgtaggctc gaaatggatc
      181 cagttcccga tttcggaaac gtcgcaactg ttcatcctga tcaatatgaa gccagaattt
      241 gacgacaacg gtgccaaaat tactgagttg ggattcaaat tcattaattt cgtggtacgc
      301 ccgcttccat tcggcctcag tgcaaaaccc ctcgatacgt tcgaccagga ctcgtccata
      361 atgcgaccgg tcaaaaattc cgatccgtcc tggatcaggt aacgtcttcc aaaatcgcca
      421 gagataatgg tgtcgggcct cagcccctga cggtgccgaa aacgtgttga cgcgaaagcc
      481 tctcggatca agcgtttccg tcactcgctt gatggcgccg cccttgccgg cggcatccca
      541 gccttcaaac agcaccagtg catttcgacg ctggttatac aaccggagtg attgctgccg
      601 caggcagaac tgccatttct tcaattcttt ggcatattct tccggggcaa ttttttttgt
      661 caggtcaact ttgtccagca tgggcagcct cctcttctct ggctgattcg gcttgcagtt
      721 gccgttccag caactctagc cgttcggtgc gcaaggcttt gccaactttc gagcggccaa
      781 tttcgccagc gtggtggtac cgccactcaa tcgcctcttc catggcccga accagcgttt
      841 caaaaatttt gacttcgcaa aaataccggt cagtcgcttc aacaatagtc cacggagcaa
      901 acggcgtgtc ggttcgagcg accatttctt caatcgcttc gacgtatttg tcatattccc
      961 gatgatgttt ccaatcccat ttggtcaccc gccagcgctc atatttgtca ttttcaagtt
     1021 tgagaaaccg acggcgctgc tcttttttgg tgatatggag aaaaaatttc accatcaccg
     1081 tcccgtcatc cgccagttgt cgttcaaatt gtagaatttc ctcaaaacca cgttgccaga
     1141 cactttcggg gaccatttcg gcgacgcgtt caatcaggac ccggccatac caggaccgat
     1201 caaagaggga aatttgacca tcttcgggta accgtaccca gtaccgccat aaaaacggat
     1261 gggcggattc ttcttcagtt ggtggatgtg acggatgcac ggtaaatcct cgtggatcaa
     1321 gccgttcaag aagtcttgaa atcatcaacc ccttgccggc ggcatcccag ccttcaaaaa
     1381 caatgacaat tggaatttcc tcatccttac atttgtattg caactgacgg agtttttctc
     1441 tcaggccagg ggcaatcgct ttatagtctt tctttttcag ctcaagcgtt aaatcaactg
     1501 tttcaagcat agtcacccac cattcaattg aagatcaaaa cacgtaagtt tccgcaattt
     1561 gacctggatc aaagcgacaa aatgaattgg atacaatcgc tgtgccattt tggagtgcgg
     1621 cggcttgacg ccgctttcca ttcattttgg agtgcggagg cttgacgccg ctttccattc
     1681 agtacccttt tgattttcgc tcggcttttt gcttgaaatc tttgacccga tgcagccccg
     1741 tccgaatatc accgttggcc agtaaatgaa tccaccgaaa ccctggattg atgtggtcga
     1801 gagcaaattc aaccgaattg ggtttaaact ggaaacaggt tgagggtgtc gccagcatca
     1861 ggacgccctt gcgggtgccg acaaattcct gatggatgtg tccccagaca atccctttca
     1921 catttgaatg gcgatcaatc acttcaaaaa aatgatctgc ctcatgaagc ccgatttcac
     1981 ccatccaatc atcagtcgtt ggcaccgggt tgtggtgcag gcacaccagc gtaaaccgat
     2041 ctggattttg ggctaaggcc tgatccagcc ggttgagttc ctcaaccgtt agccggccat
     2101 gaacctcgcc ggggacattg gaattgagca tcaatacctg ccactccttg atcttaaaag
     2161 cattggccgg attgatcggt tccgtcaccg ggtgactggc catcatttca acttcatcgt
     2221 gatttccagc cagccagtac actgggatga gtggcgccga ctcggttttt ctcaattgcc
     2281 gacaaaaagt ctcaaaacac gactgcagca ggtgataggt ttcggcggtg gcgtgctggg
     2341 ccaaatcgcc agtcgccagg atggcgtcag ggatatcctc ttgctccagg gccagttcaa
     2401 aaacctgttg caacgaatca aacgtgttga ttcccaagag tttaccatca cgcgacggaa
     2461 acagatgcag gtcagtaaat tgaaatagca tcacagacga tagatgtttt gcagcagaca
     2521 cgacgggtgt tggctctggc ataaggtgag ttcttcctct tttttgaacg atgtagctcg
     2581 aaatcaaagg gatgattcga atctggatgc ggcgttgtgg tacaaactgc tggaattcag
     2641 gctgcatgtt ttctgcgggt tgagtgtaaa atggccgaac tgatcaggtt tcggccttgg
     2701 gcccaaaatg aagccccttc cttttccacc cacatctgaa gtctaccctc attgactgga
     2761 ttttccttca gatgagccga agaaatcacg agtttttact ttacctaata accaacctcc
     2821 agtcaaaaaa ggagatagaa agccttggca tacgaaaaca ttttagttga tattcaaggg
     2881 ccggttggca ttgttgtgtt aaaccgtccc cagtcactca atgccattga taaagccacg
     2941 attgtggagc tgctcggggc atttgaaacg ctgcgtgata ctgacgcagt gcgggccatc
     3001 gtttttacgg gtgcgggccg ggcattttca tccggcggcg acatcaagga aatgatccgc
     3061 tcgcaagggg ccacctcaga cgaggtcttt cgtgagcaca ttggggacat caatcggaca
     3121 atttcactga tgggcgaaat tcccaaaccg gtcatc
//
LOCUS       DAOQDO010000508         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24064_length_3150_cov_3.793215, whole genome shotgun sequence.
ACCESSION   DAOQDO010000508 DAOQDO010000000
VERSION     DAOQDO010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24064_length_3150_cov_3.793215"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="PKZ53_09275"
     CDS             <1..1735
                     /locus_tag="PKZ53_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40668.1"
                     /translation="ITLLGGIITTTWQARVAQAERAKSERRFNELRKLARAFIFDFHD
                     KIENLQGSTPARELLVKQGLEYLDNLATEASGDQLLQYDLATAYQKVGDIQGRPYAPN
                     LGDTAGAVQSYRKSLEIFEKLTQDNPQNQQFAFDLSIGTERVGDILARTGDTAGAIQH
                     YTQAFDLQRTLLAAAPLNGEYQKHLAMCFTKLGDTLTTTGDLIGALDKYQQALSIRES
                     LLTSGLPQEQIKRAQAVSYYRIGNTLEAIADLTASRVGDLPEVQAIYQEALSAHQKVQ
                     AIMEELSTTDHTTARTQHDVASSLIHVSKLLAKTGQPKEALQPAQKALDLCEKLADAD
                     PQNVEIRSQLSLTNHWFGDIDLAAGNPASALKKYQTARLLREMLLSSDPTNAEYRQFL
                     VSLHQALGKVYSQTGETEKALAHFRQALELGEATLAASPSNVVLGRQCSQLLNQLTAV
                     LVKAGRQAEARQITLARLTTRKTQVEQPRATPWELTEYAWLLLTCEPAEFRNPALAIE
                     YARRANLQTQETNLAALQVLALAYTRLNDPIRATETITRFLQLLPISPSLPEKSRSQT
                     IEMALEKYKRG"
     gene            complement(1732..2526)
                     /locus_tag="PKZ53_09280"
     CDS             complement(1732..2526)
                     /locus_tag="PKZ53_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40669.1"
                     /translation="MTTTQTTEITLTVTGLCPMIFDSDNERAAFGIFHDRDHQTVLKV
                     DNVDWSQSFNGIASLTVHRALGSHRQNRIVRKADNVVTDPSQVPAGDLSFSHVVDIQK
                     NLYGGLHLPMQTGLLKARLILNDGEFSVSKLFNGGAPGAPPRKVWFVPENNLSKVVYE
                     NYVAEELMVRIYLEKDDFAFLTFDNGDFVYLYGDQNHSIVMSNMCDPDVIASGQDDFI
                     LQYNLYRMGDHPRVIPQYDPPGTIAPRQGTSMCIGGSNCIPPGSCT"
     gene            2783..>3150
                     /locus_tag="PKZ53_09285"
     CDS             2783..>3150
                     /locus_tag="PKZ53_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40670.1"
                     /translation="MAELSPSKNIWSVVANGEVIGHEQILTTHNLATLVSSLGLIEKL
                     NPHLEELARRCFNWICTRHQQKISLWNAQLQMIKNTAYAWRQLVFFLALLPEDTQTGF
                     LTWAYTHLKSQRPAYQTCFKP"
BASE COUNT          844 a          866 c          746 g          694 t
ORIGIN      
        1 catcaccctc ctcggcggga tcattaccac cacctggcag gcacgtgtgg cacaggcaga
       61 gcgtgccaaa tccgaacgcc ggtttaacga gctgcgaaaa ctggcccgcg ccttcatctt
      121 tgattttcac gataaaattg aaaacctgca gggctcgacc ccagcccgcg aattactggt
      181 caaacagggt ctggaatatc tggataatct cgctactgaa gcaagcggcg atcaattgtt
      241 acaatatgat ctggcgacgg cctatcaaaa agttggtgat atccagggcc gcccatatgc
      301 accaaatctc ggagacacgg ctggagccgt acaaagttat cgcaaatcac tggaaatatt
      361 tgaaaaactg acccaggaca atccgcaaaa ccagcagttt gcgtttgatt tgagcattgg
      421 aactgagcgt gtcggagaca ttttggccag aactggagac acagccgggg caattcaaca
      481 ttacacccag gcattcgatc tgcaaagaac actgcttgcg gcggcccctt taaacggaga
      541 gtatcaaaaa catctcgcga tgtgctttac caaactcggt gatacgttaa ccaccactgg
      601 agatctgatc ggagccctcg acaaatacca gcaggctctc tctatccgtg agtcattgct
      661 gacctctggg cttccccagg agcaaatcaa acgtgctcag gcagtgagct attaccgcat
      721 tggcaatacc ctggaagcca ttgccgacct gacggccagc cgggtcggag acctgcccga
      781 agtccaggcc atctatcagg aagctttgag cgcacaccaa aaagtgcagg ccatcatgga
      841 ggaactttca accactgacc acaccacagc cagaacccag catgatgtgg cgtcgagctt
      901 gattcatgtc agcaagctgc tggcaaaaac cggccagccc aaggaagcgc ttcaacctgc
      961 tcaaaaagcg ctggacttgt gtgaaaaact ggcggatgcc gaccctcaaa atgtggagat
     1021 tcgctctcag ctctccttga ccaaccactg gttcggagat attgaccttg cggctggaaa
     1081 tccagccagc gccctgaaga aataccagac ggcacgctta ctccgggaaa tgctcctgag
     1141 cagtgatcca accaatgctg aataccgtca atttctggtg agcctgcacc aggcactggg
     1201 aaaagtgtat tcccaaactg gcgagactga aaaagcgctg gctcatttcc gacaggcgct
     1261 ggaacttggt gaggcaacac tggcggccag cccatcaaat gtggtgcttg gccgccagtg
     1321 ttcccagctt ctcaatcaac tcaccgccgt tttggtcaaa gccgggcggc aagccgaagc
     1381 ccggcaaata accctggcgc gtctcaccac acgcaaaacc caggttgagc aaccccgcgc
     1441 aactccctgg gaattgaccg aatatgcctg gctgctgctg acctgcgaac cagcggaatt
     1501 tcggaacccg gcgctggcga ttgagtatgc ccgtcgggca aacctgcaaa cccaggaaac
     1561 caatctggca gcgctccagg tcctggccct ggcctatacc cggctcaatg acccaatccg
     1621 cgcaaccgaa acgatcacca ggtttttgca actcctgccc atttcgcctt cgcttccaga
     1681 aaaatcgcgt tcccaaacca ttgaaatggc cctggaaaaa tacaaacgag gctaagtaca
     1741 cgaccccggc ggaatacaat ttgaaccacc aatgcacatt gacgttccct gtctcggagc
     1801 aatggttcct gggggatcat attgcggaat tacccgagga tgatctccca tccgatagag
     1861 gttgtactgc aggataaaat cgtcctggcc ggatgcgata acatcaggat cacacatatt
     1921 gctcattaca attgaatgat tctggtcacc gtagaggtat acaaaatcgc cattgtcgaa
     1981 cgtaagaaag gcaaaatcat ctttttccag atagattcgg accatgagtt cttcggcgac
     2041 ataattttca tacaccactt tgctcagatt attctcaggt acaaaccaca cttttcttgg
     2101 cggagcccca ggggcaccgc cgttaaagag ttttgaaacg gaaaattctc cgtcatttaa
     2161 aatcagccgg gccttgagta aacctgtttg catcggcaaa tggagtccgc cgtataaatt
     2221 tttctggatg tccaccacgt gactgaaact caaatcacct gctggaacct ggcttgggtc
     2281 cgtcactaca ttatctgctt tgcggacaat tcgattttgg cggtgtgacc ccagcgctcg
     2341 atggaccgtc aacgatgcaa tgccattgaa agactggctc caatctacgt tgtcaacctt
     2401 gagaacggtc tggtgatcac gatcgtgaaa tattccaaaa gccgctcgct cattatcact
     2461 gtcaaaaatc atggggcata acccggttac cgtgagggta atttctgtcg tctgagtagt
     2521 tgtcataaca ctccttccct ggtcagcttg ttactgacga aatgtcaggc acactggcgc
     2581 tgaagatctt tcttcacttc acttgcaagc aggcaaagag gcattacctt ctgctttcaa
     2641 gtgtttaacg ggtgcatttg agccagagcc agatgggcag gaaaaagctc tcgctactat
     2701 catgaagcga agaaaaacgg tgaacggggc caacacagga aaaaattgtg cagaaaccat
     2761 cagatctcag tctgaatcct gaatggctga gctttcgcct tcgaaaaaca tctggtcagt
     2821 ggtcgccaac ggggaagtta ttggacatga gcaaattcta accactcata acctggcaac
     2881 actggtttcc agccttggac tgattgaaaa gctgaatcca catctggaag agcttgcccg
     2941 gcggtgtttc aactggattt gtacccgcca ccagcaaaag atcagtctct ggaatgctca
     3001 acttcagatg atcaaaaaca cggcttatgc ctggaggcaa ctggtcttct ttctggctct
     3061 gttgccggaa gacacccaaa ctggatttct gacctgggct tacacccacc tgaaatcaca
     3121 acgccctgcc taccagacgt gtttcaaacc
//
LOCUS       DAOQDO010000509         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24119_length_3146_cov_2.926237, whole genome shotgun sequence.
ACCESSION   DAOQDO010000509 DAOQDO010000000
VERSION     DAOQDO010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24119_length_3146_cov_2.926237"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..715)
                     /locus_tag="PKZ53_09290"
     CDS             complement(92..715)
                     /locus_tag="PKZ53_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40671.1"
                     /translation="MKTGRLQLRSGIKPPLPRPMVTVITTTTILVGIGAAVAVWVGMT
                     PALWVKPVRTEPIQLAAAFSNQPFAGVKHFQFSGLERPKLSDFLPRFERKTTDMLVEQ
                     MPISQGESSKRNRKETDPSDIFPMPHPAPSLNPNFTPDDYTAPVGTSLILLGHRLWQQ
                     TIETDPKELGKSLNLDNPDYISVGVLPQALSWAPEDEPEKPEPTFQN"
     gene            complement(864..1487)
                     /locus_tag="PKZ53_09295"
     CDS             complement(864..1487)
                     /locus_tag="PKZ53_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ40672.1"
                     /translation="MSKLTLSCFLFATMFFTGCHTLAHSHGEANHGAVASSEASIGVA
                     APAFAGTDTSGKTHRLSDYKGKFVVLEWFNPECPAVRPHYANNAMQNLQRTYTKKGVV
                     WLTIDSSAPGKQGHLTPDQGEAVLKERHAAPTALILDADGKIGRTFGAKTTPHMFVID
                     PNGKLIYAGAIDDRNGKNFVQAALDEALAGKPVSVPATQSYGCSVKY"
     gene            complement(1524..2117)
                     /locus_tag="PKZ53_09300"
     CDS             complement(1524..2117)
                     /locus_tag="PKZ53_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40673.1"
                     /translation="MNIPVFLKLNKRFLTLCSLVFVGLMGVISCASHADDQSKPQAEK
                     LSWTTVIAGKDEPGERLRVSGRVLQADRKTPVAGIVVEVHHTDAQGLYNPKGTGQAPP
                     RLRARLQTNAQGQYEFHTIQPGPYPDGGNPAHIHFAVYREGEPKQFSEIFFAGDPNLT
                     EAFYKEMRDEKANYVVKQPVRNNQGVLECTYDIILSH"
     gene            complement(2205..2909)
                     /locus_tag="PKZ53_09305"
     CDS             complement(2205..2909)
                     /locus_tag="PKZ53_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40674.1"
                     /translation="MNTFSRRHFFQLGLFGAVSVGIPDLLMPPGLAPTETTDDLDHYK
                     EHLVISEKAKPGSPWAATEFVDQGPFYIAGAPYRAKLSPPLAEGTVLVVKGRVWDFKT
                     RKPVPMALLDVWQADAGGVYDFENNDDRRPPKEGMTRNRARLMTDESGYYEFETIHPG
                     AYQIGPTRWRPSHIHYWIKHPKYKELVTQIFFKGDDHHQGDFAWRESLMIDLQRQSIR
                     GATLESGTFDIILEPK"
BASE COUNT          784 a          771 c          778 g          813 t
ORIGIN      
        1 aaaaattata gccttcaaac gaaaaaggaa agatggtttt caaaaccacc tttccttttt
       61 atttgattcg aatttggcag acaacctgag attagttctg gaacgtcggc tctggttttt
      121 ctggttcgtc ttctggtgcc cacgaaagcg cctggggtaa cacaccgacc gaaatataat
      181 ctggattgtc aagattgagc gatttcccta actctttcgg gtcagtttcg atggtttgct
      241 gccacaaccg atgacccagc aaaatcaggg acgtaccaac cggcgctgtg tagtcatcag
      301 gggtaaaatt tgggttcaga ctcggggctg gatgcggcat cggaaagata tctgaggggt
      361 ctgtttcttt ccgatttcgt ttggaagatt caccctggga aatcggcatt tgctcaacca
      421 gcatatccgt tgttttgcgt tcaaaccggg gcagaaaatc agacaacttt ggtctttcca
      481 aacctgaaaa ctggaaatgt ttcaccccag caaacggctg gtttgaaaaa gcagccgcaa
      541 gctggattgg ctcagttctg acgggtttaa cccacaaggc aggggtcatc ccaacccaga
      601 cggccacggc ggccccaata cccaccaaaa tcgtggtggt tgtaatcaca gttaccattg
      661 gacgaggcaa tggtggcttg ataccagagc gaagttgtag ccgaccggtc ttcatggcgt
      721 gtctccgtaa tcaaatcttt ctcaacagta tgtggtggaa acttaatttg tgaaactacc
      781 atgaggactt cagatgaaca agcgaactga gaaaatagcg aaggaggagc gacgcgatct
      841 tccttcgcta cttctcgatc aactcaatac ttgaccgagc agccatatga ctgagttgct
      901 ggtacggaga cgggtttgcc agccagggct tcgtcgaggg cagcctggac gaaattcttt
      961 ccgtttcgat catcaatcgc accggcataa atcagcttgc cattcgggtc aatcacaaac
     1021 atatggggcg tggttttggc gccaaaggta cgaccgattt ttccatctgc atccaggatc
     1081 aaggcggttg gtgccgcatg gcgttccttg agtaccgcct ccccctggtc aggcgttaaa
     1141 tgaccttgtt tccctggtgc cgaagaatca attgtgagcc acacaacacc ttttttggtg
     1201 tatgttcgct gcaaattctg cattgcatta ttggcataat gtggccgaac tgccggacac
     1261 tcagggttaa accattccag aactacaaac ttgcccttgt aatctgacaa ccgatgggtt
     1321 ttgccgctgg tgtctgttcc agcaaatgcg ggcgccgcaa caccaattga agcttcggaa
     1381 ctggcaacgg cgccgtgatt ggcctctccg tggctatgag ccagtgtatg gcatccagtg
     1441 aaaaacatgg tggcaaacaa gaaacatgag agtgtcaatt tggacatggt cggtctcctt
     1501 tagaagtaat ttgttgtcgt tggttagtga ctcaggatga tgtcgtacgt acattcgagc
     1561 acaccctggt tgtttcgaac tggttgtttc acaacatagt ttgccttttc atcgcgcatt
     1621 tctttgtaaa aggcttcggt cagatttggg tcaccggcaa aaaagatttc agaaaactgt
     1681 ttcggctcgc cctcgcgata tacggcaaag tgaatatggg ccgggttgcc gccgtcaggg
     1741 taaggccctg gttgaatcgt gtggaactca tattgcccct gggcattggt ttgaagcctg
     1801 gcccgcagtc gaggcggggc ctggccagta cctttcgggt tatataaccc ttgagcatca
     1861 gtgtgatgca cttcaaccac aattcctgcc actggtgttt tcctatcggc ttgcaaaact
     1921 cggcctgaaa cccgaagccg ctctccaggc tcatccttgc cagcgatcac ggttgtccag
     1981 gagagttttt ccgcctgtgg tttggattga tcatcagcgt gactcgcaca actaatcaca
     2041 cccatcaatc caacaaaaac cagagagcaa agcgtcagaa accgtttgtt caatttgaga
     2101 aaaacaggaa tgttcattga gtgacctcat cattctaacg ggtgaagatt gcgtgtctcc
     2161 ctgatggatt gacacctcac tatttcacta cttcgtgact ccggctattt cggctccaaa
     2221 atgatgtcaa aggtaccact ttcaagcgtt gcacctcgaa tagactgtct ttgaaggtca
     2281 atcatcagcg attcacgcca ggcaaaatca ccctggtggt gatcatcacc tttgaaaaag
     2341 atctgggtaa ccagttcttt gtatttcgga tgtttgatcc agtaatgaat gtgactgggc
     2401 cgccagcgag ttgggccgat ctgataggcg ccaggatgga tggtttcaaa ttcataatac
     2461 cctgattcat ccgtcatcag gcgagcccga ttgcgggtca tgccttcttt cggaggccgt
     2521 cggtcatcat tgttttcaaa gtcatacacc ccgccggcat cagcctgcca gacatcgagc
     2581 aaggccattg gcaccggctt tcgggtttta aaatcccaaa cccggccctt gaccaccagc
     2641 actgtgcctt cagccagcgg aggtgaaagc ttggctcgat acggagcgcc agcgatataa
     2701 aacgggccct ggtctacaaa ttcagttgcc gcccagggcg accccggttt ggctttctcc
     2761 gaaatgacca gatgctcttt gtaatgatcc aaatcatctg ttgtttcagt cggagccagg
     2821 ccaggcggca tcaacaggtc tggaattcca acggaaaccg cgccaaataa accaagttga
     2881 aaaaaatgcc gacgtgaaaa tgtgttcata cgggactcct tcatttttcc aataccagtt
     2941 tgtagtcagt agtcagtagt cagtagttag tagccttcta agcttctttg attgaatgac
     3001 ttgactgctt tctaaaacaa gggtttcatc gtaaaagggt acaagtcagc ggtaagtcgc
     3061 cagcagaggg ttcggaaaga tttattcggt gttcctggcc gacaaaatga ttgaaaattg
     3121 agcccaggcc gtgatggaca aaccac
//
LOCUS       DAOQDO010000510         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24141_length_3144_cov_3.056329, whole genome shotgun sequence.
ACCESSION   DAOQDO010000510 DAOQDO010000000
VERSION     DAOQDO010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24141_length_3144_cov_3.056329"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..2722
                     /locus_tag="PKZ53_09310"
     CDS             323..2722
                     /locus_tag="PKZ53_09310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ40675.1"
                     /translation="MPSIRRNFQWTLVGNSIFALSQWLVLVIITKSGNPEMAGMYALG
                     LAVTTPIFSFANLNLRAVQATDASQRFTFSDYVAVRLMTSVLALISIVALVWMLPYAT
                     EVRLVIVGIAGAKFFESLSDIVYGKWQFEEAMPNISLSMILRGVSTVLVVWLVFTLSQ
                     NLVVTMASMAVVWGLTLVGFDIAGIKPQAFGIKTQNSELGDRNPLLSLIWLALPLGGV
                     MLINTLNVNLPRYWVERALGLPQLGILAALFSLVLVGTTLINALGQAASPRLAKLYAA
                     GRKQDFIRLLAGLAASALAIGVGGLLVSIVFGKPLLAILFTRHYAAYTHLLIWVMVYG
                     LVTYLSSVAGYGVTSARFFRIQVPWNSLEMLCLMAACFVLIPRFGLSGAVWSSTLAAL
                     VHLSLKIGTLWSAVRRCDSQPQRLNEERDLPAAPIRILHVFGQMNRGGAEMRTVELLK
                     SLNKQEFEFHFATLSGLPGDLDHEITRLGGVIHPLGLGIRFPVQFFRLIKTSGCQVVH
                     SHVHYFSGAILCIARLAGVNVRIAHFRSVSDGRLPTLRRRIQTTVMGWLIDCFATNIL
                     AVCQGAMDASWKQWHTDQRCEVVYNGLDTSRFREPSDRQGVCREFGLDPEAPFFIHVG
                     RMDPAKNHVRLMTIFHQVLRRLPAARLVLVGRTTPDIQLQVNQLAQTLGIADRLVFAG
                     VRSDVPRLLKAADLMIFPSRWEGLPGAVLEACAAGTPVVASNLPGVKEIASEVGRVWE
                     VSLDESDAQWADRVSDLFHQSQTTRNRVGSMTKFEQSQFSISACLQELYTIWRGGTTH
                     V"
     gene            2715..>3144
                     /locus_tag="PKZ53_09315"
     CDS             2715..>3144
                     /locus_tag="PKZ53_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40676.1"
                     /translation="MFNPLPVFQNSYQPRSVLPLTVDAPAVVPAARPETMPATLLVLA
                     LVGFGIGVVLAIQDTWLNLSFDDFAFCISLCAVVCLAGTARLVKGGVSSAPFVFLVVF
                     SVFHFGALVIFGLGIPFSDAQNYTLQQWFFSSDTQLGGYLS"
BASE COUNT          609 a          773 c          869 g          893 t
ORIGIN      
        1 ggtatcgggc taagggctga gggaaataca attctttcaa caatttagcc ttctcataat
       61 acgagggctt catcccaata acaagtggta ttataagtat cttgtcataa attttctgag
      121 atattgggtt tggtaagtgc ttgattcttt tccgtgtttt ccgtgttttc cgtggttaaa
      181 atgtttggaa attttcggta aggtacttag caaatagctg aatcattcag agaattgtag
      241 ttcccttata ttccaaaccc tgaaccctga accctcaata gtcttacttt ttcaacaact
      301 ctccagagag gtgtgagggc ttatgccgtc aattcgccgc aacttccagt ggacgctggt
      361 tggaaacagt atttttgccc tttcacagtg gctggtgctg gtgatcatca ccaaatccgg
      421 taatcctgaa atggccggta tgtatgccct gggcctggcg gtgaccaccc ccattttttc
      481 atttgccaac ctcaatcttc gtgcggttca ggcaaccgat gccagccagc ggtttacctt
      541 ttctgactat gtagcggtcc gattgatgac gtcagtgctg gccttgatca gtattgtcgc
      601 tctggtttgg atgttaccct atgccactga agttcggctg gtaatcgtcg ggatcgccgg
      661 ggcaaaattc ttcgaatcct tgagcgatat cgtgtatgga aaatggcagt ttgaagaagc
      721 catgcccaat atttcgcttt cgatgatcct gcgtggtgtt tcaacggttc tggtggtgtg
      781 gctggtcttt accctgagtc agaatctggt tgtaacaatg gcttcgatgg ctgtggtctg
      841 ggggctgacc ctggttgggt ttgatattgc aggtatcaaa ccacaggcgt tcggaataaa
      901 aacccagaat tcggaactcg gggatcggaa tccgctcctt tcgctcatct ggttggcgct
      961 cccgctcggg ggggtgatgt tgatcaatac cttaaatgtc aacctgccac gatactgggt
     1021 tgagcgtgcg cttgggttac cgcagcttgg aatcctggcc gctttgtttt ctctggttct
     1081 ggtgggaact accctgatca atgccctggg ccaggccgcc agtcccagac tggcaaaact
     1141 gtatgcggct ggccggaaac aggactttat ccgattgctg gctgggctgg ctgcttcggc
     1201 actggcgatt ggcgtgggtg ggttgctcgt ctcgattgtg tttggaaagc ccctgttagc
     1261 catccttttt acccgccact atgccgccta cacgcatctt ctgatctggg tgatggtcta
     1321 tggactggtg acatacctca gttcggttgc cggctatggc gttacctcag cccggttttt
     1381 ccgaatccag gtgccgtgga acagccttga gatgctgtgt ttgatggctg cctgttttgt
     1441 tttgatccct cgatttgggt tatctggagc cgtttggagc agcacgctgg cggctcttgt
     1501 ccatttgagc ttaaagatcg gaacgctgtg gagcgcggtg cggcggtgtg actctcagcc
     1561 tcagcgcctg aacgaagaac gtgacctccc ggcggcgccg attcgaattc tgcatgtgtt
     1621 tgggcaaatg aatcggggtg gtgccgaaat gcggacagtt gaactgctca agtcactcaa
     1681 taaacaggaa tttgagtttc actttgccac tctctctgga ttgcccggtg atttagatca
     1741 tgaaatcacc cgccttggcg gggtaatcca tcccctgggg ctgggcattc gatttccagt
     1801 acagtttttc cggctgatca aaaccagcgg gtgtcaggtg gttcattcgc atgtgcatta
     1861 tttttcaggt gctattttat gtattgcccg actggccggg gttaatgtac gcattgccca
     1921 ttttcgaagc gtctctgacg gacggctgcc aacactgcgc cgccggatac agaccacggt
     1981 catgggctgg ttgattgatt gctttgcaac caacatttta gccgtttgcc agggggctat
     2041 ggacgcttcg tggaaacagt ggcataccga ccagcggtgc gaggtggtct ataacggtct
     2101 cgatacctcc cgatttcggg agccgtcaga tcgacagggg gtatgtcggg aattcggact
     2161 cgacccggag gctccatttt tcatccacgt tgggcgcatg gatcccgcca aaaatcatgt
     2221 tcgactgatg acaatttttc accaggttct gcgtcgcctg cctgcggcca gactggtgct
     2281 tgttggcagg acaacgccag atatccagtt gcaggtaaat cagttagctc aaaccctcgg
     2341 gattgcggac cggctagttt ttgccggtgt gcgcagcgat gtcccgcgac ttctcaaagc
     2401 ggctgacctg atgattttcc cttcccgatg ggaggggttg cccggtgcgg tcctcgaagc
     2461 ctgtgctgcc gggacgccag ttgtggccag caatttgcca ggagtaaaag aaattgcttc
     2521 tgaagttggt cgggtgtggg aggtatcact tgatgagtca gacgcccagt gggctgaccg
     2581 ggtgagtgac ctgtttcacc aatcccagac caccaggaat cgagtcggaa gcatgacgaa
     2641 atttgaacaa agccagttca gcatttcagc ctgtcttcaa gagctgtata ccatctggcg
     2701 cggaggaacc acccatgttt aacccactcc cagtttttca gaactcatat cagccccgtt
     2761 cggttctccc gctgacggtt gacgcgccgg cggttgtccc cgccgcccgg cctgaaacga
     2821 tgccagccac gttgctcgtg ctggcgctgg tgggtttcgg gatcggagtg gtgcttgcca
     2881 ttcaggacac ctggctaaat ctcagcttcg atgattttgc cttctgtatt tcactctgtg
     2941 ctgtagtgtg tctggctggt actgccagat tggtcaaagg cggcgtttct tcagccccat
     3001 ttgtatttct ggtggtcttc tcagtgtttc attttggggc gctggtcatt tttgggcttg
     3061 gaattccatt ttcagatgcc cagaattaca cgttgcagca atggtttttt agttcggata
     3121 cgcaactggg tgggtatctc tcgt
//
LOCUS       DAOQDO010000511         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24151_length_3143_cov_3.542422, whole genome shotgun sequence.
ACCESSION   DAOQDO010000511 DAOQDO010000000
VERSION     DAOQDO010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24151_length_3143_cov_3.542422"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..356)
                     /locus_tag="PKZ53_09320"
     CDS             complement(96..356)
                     /locus_tag="PKZ53_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40677.1"
                     /translation="MTDTPPEIQKILDERFQALTPTERMIMASQMFDSARQIILASFP
                     PDLSEIEVKRRLCERLYGDEVDVEAFINYYLGRKTRTRMLDP"
     gene            complement(353..898)
                     /locus_tag="PKZ53_09325"
     CDS             complement(353..898)
                     /locus_tag="PKZ53_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40678.1"
                     /translation="MMTTELEILKEICQKLERAGIDYMLTGSFAMIHYTQPRMNRNID
                     FVISLETQSLERFVGLFDSGFYLSKGAIQRAVHIRSMFNLIHLESIVKIDFVVLKNEP
                     YQQEEFARRKRVPLADFEVWIVSLEDLILSKLIWAKPTNSELQLRDVKNLLKDTYDEA
                     YLYEKARSLGVFELLEMVLRQ"
     gene            complement(923..3076)
                     /gene="ppk1"
                     /locus_tag="PKZ53_09330"
     CDS             complement(923..3076)
                     /gene="ppk1"
                     /locus_tag="PKZ53_09330"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNJ40679.1"
                     /translation="MARSDTRPLQPINLEDSAVEFVPPSFSGSRHLLFNRELSWLEFN
                     RRVLQEGLDPSQPLLERLKFLAIFSTNLDEYFMIRVSGLQEKLEEEVQELSPDGMTPF
                     EQLREIRHRTLPLLAEQMKCLRTEVMPQLAEAGIVVSPYTELSRAEKKSADHYFHEKV
                     FPVLTPQAVDPSHPFPYISNLSLNLAVMVEPTQCSEIGLTGVRFARIKLPPMVPRLVP
                     VDASGTKFTLLGSLIAANANTLFPDMNYGRFHLFRVTRDADIEIREDEAGDLLRFMQQ
                     KLSRRRRFGAAVRLEVASTMPSDMVDYLMASLDLTPEDVYVIDGPLNIPDFMQLLSLD
                     RPELKDRPMQVVVPAPLRQQKKKNIFDLVKKQDILLHHPYNSFSTVMDLIDMAARDPQ
                     VVAIKMCLYRIGRHSPIVQSLISASEQGKQVAVVVELKARFDEENNIEWAKQLEQAGV
                     HVVYGLMGLKTHCKVALIVRNEGDSLRTYVHIATGNYNAATAKIYTDLGIFTADQEIG
                     FDATNLFNYLTGYSRYSNYRRLMVAPVNLRQRFFALIERETEHARAGRQGHIIAKMNS
                     LTDMETIQALYRASQANVKVELIIRGICCLRPGVPGFSENITVRSIVGRFLEHSRIYY
                     FSNNGDEEVFIGSADWMGRNLSRRVEVVAPVLNPELRKYLRDDVLNAYLRDNLKARIL
                     QPDGTYVQTKPSETEEPFSSQLYFANLPQNEIDED"
BASE COUNT          802 a          753 c          792 g          796 t
ORIGIN      
        1 gcaactgatt ggatctgaac cgcttggtgc cggcaagatg ccggcgttcc caggagggaa
       61 ttgttttctg aagagtgata gattctttca gacactcagg gatccagcat tctggttctg
      121 gttttccgtc cgaggtaata gttgataaaa gcctctacgt ccacttcatc gccatacagc
      181 cgctcacaaa gacgccgttt gacttcaatt tcagataaat ccggtgggaa cgacgccagg
      241 atgatttgcc gggcgctgtc aaacatctga cttgccataa tcatccgttc agttggggtc
      301 agtgcctgga agcgttcgtc gaggattttc tgaatttcag gtggtgtgtc ggtcattgcc
      361 ttaaaaccat ttccaatagt tcaaaaaccc caagcgacct ggctttttca tacaggtaag
      421 cctcgtcata agtgtccttc aataagtttt tcacgtcccg aagttgaagt tcagagtttg
      481 tcggtttggc ccaaatcagt tttgagagaa ttaaatcttc caggctgaca atccacactt
      541 caaaatcagc caggggaact cgttttcggc gggcaaattc ctcctgttgg tacggttcgt
      601 ttttgagaac cacaaagtca atttttacaa tgctttcaag gtgaatcagg ttgaacatgg
      661 atcgaatatg cactgcccgc tggatagcac cttttgacag ataaaatcca gaatcgaaca
      721 atccaacaaa ccgctcaaga gattgagtct ccagagaaat cacaaagtca atattgcggt
      781 tcatccgggg ttgggtatag tgaatcatgg caaaagaacc agtcagcatg tagtcaatcc
      841 cagcccgttc cagcttttga cagatctctt tgaggatttc caattcagtc gtcatcataa
      901 gagattgacc cggagcaacc cgttaatctt catctatttc attttgcggc agattggcaa
      961 aatagagctg tgaactaaat ggctcttctg tttcagaagg tttagtctgc acataggtgc
     1021 catctggttg aagaatccgc gctttgagat tgtcgcgaag gtaggcattg agtacgtcgt
     1081 cacgcaggta tttccttaac tccggattga gaactggtgc cacaacttca actcgacggc
     1141 tgagattacg tcccatccag tcagcgcttc cgatgaaaac ctcttcgtcg ccattgttgg
     1201 aaaaatagta aatccggctg tgctccagaa accgaccgac aatgctgcgg acagtaatat
     1261 tttcagaaaa tccgggaacg ccaggccgca aacagcaaat tccccgaata atcaactcga
     1321 ctttcacatt ggcctgtgac gcccgataga gtgcctggat cgtttccata tccgtcaagc
     1381 tgttcatttt ggcaatgata tggccctggc gaccagcacg ggcatgctcg gtttcacgtt
     1441 cgatcaaggc aaaaaaccgc tggcgtaaat tcactggcgc gaccatcaat cgccggtaat
     1501 ttgaatagcg cgaatacccg gtcaaataat taaagaggtt ggtggcatca aagccgattt
     1561 cctggtcagc ggtaaaaatt cccaaatcgg tataaatctt ggctgtggcc gcgttgtaat
     1621 ttccggtggc aatgtgcaca taggtccgga gcgaatcacc ttcgttgcgc acaatgagcg
     1681 ccactttgca gtgcgttttg aggcccatca acccataaac aacgtggacc ccagcctgtt
     1741 caagctgctt ggcccattca atattgtttt cttcatcaaa ccgggctttg agttcaacca
     1801 ccaccgcgac ctgtttcccc tgttcgctgg ccgaaatcag agattgcaca atcggtgaat
     1861 gccgcccgat tcggtaaagg cacattttga tcgccaccac ctgtggatca cgggcggcca
     1921 tatcaatcaa atccatcacg gttgaaaacg agttgtatgg atgatggagc aaaatgtctt
     1981 gctttttgac caggtcgaag atgttctttt tcttttgttg acgcaacggt gccggaacca
     2041 caacctgcat tggacggtcc ttgagttcgg gccgatccag tgacagcagt tgcataaaat
     2101 cgggaatatt gagcggtcca tcaatgacat acacatcttc tggggttaag tcgagtgatg
     2161 ccatcaaata gtcaaccatg tcactgggca tggttgaggc gacttcgagt ctgacagcgg
     2221 cgccaaaccg acgccggcgg ctgagttttt gctgcataaa ccggagcagg tcgccggctt
     2281 catcttcgcg gatttcaata tctgcgtctc tggtgacccg aaagaggtgg aaccggccat
     2341 aattcatatc cggaaacagg gtgttggcat tggcggcaat caggctgccc aataaggtaa
     2401 atttggtgcc tgaggcatca accgggacca ggcgcggtac cattggaggg agcttaatgc
     2461 gggcaaagcg cacgccagtt aacccaattt cggaacattg agttggttca accatcacgg
     2521 ccagattcag gctcaggttg gaaatgtagg gaaatgggtg gcttggatcc acggcctggg
     2581 gcgtcagcac gggaaacact ttttcatgga aataatggtc cgcgctcttt ttttcagccc
     2641 tggacagttc ggtatagggg ctcacgacaa tcccggcctc ggccagttgg ggcatgactt
     2701 cggtgcggag gcacttcatt tgctcggcca gcaacgggag cgttcgatgt ctgatttcac
     2761 gcaactgctc aaacggtgtc atcccgtcgg gtgataattc ctggacttct tcttcgagtt
     2821 tttcctgcag cccagagacc cggatcataa aatattcgtc caggttggtc gaaaaaatcg
     2881 ccagaaattt cagccgttcg aggagcggtt gggatggatc cagtccttcc tgcagtaccc
     2941 gacggttaaa ttcaagccag cttaactcgc ggttaaacag gagatgacgt gatcctgaaa
     3001 aggaaggtgg aacaaattca acggcggaat cttccaggtt gatcggttga agcggacggg
     3061 tatctgaacg tgccataagg gaatgaagac tgaagaatga aaaattgtga aatgattgga
     3121 attgcgtgat atgcgagcca gaa
//
LOCUS       DAOQDO010000512         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24227_length_3138_cov_3.117418, whole genome shotgun sequence.
ACCESSION   DAOQDO010000512 DAOQDO010000000
VERSION     DAOQDO010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24227_length_3138_cov_3.117418"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PKZ53_09335"
     CDS             <1..1000
                     /locus_tag="PKZ53_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNJ40680.1"
                     /translation="VLCNGRKHLDALVNGEQAQGEFYIPLATSEGTLVASYSRGMKVI
                     NLSGGATVTVTADQMQRAPVFIFRNAREARDFANWIRDNLDQIRTVAESTSRIAKLVS
                     IEFYQSNKFLYTRFNYSTGDAAGQNMVGRATYAASKWMVEAHGGVTRWFLESNFATDK
                     KGSQINSIMTRGKRVTAECTIPPEVLKQVMRTTPEQLHYHYSVANVAGFLSGVNNNGA
                     HSANAITAMFIATGQDVANVAESSAGVLYCEVTDNGSLYISLTIPSLIVATFGGGTGL
                     PTQQECLGILGCQGRGKVNKLAEIIAAVALAGEISLSSAISSHDWVSSHEQYGRNR"
     gene            1082..2158
                     /locus_tag="PKZ53_09340"
     CDS             1082..2158
                     /locus_tag="PKZ53_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40681.1"
                     /translation="MPKKTKAQKMKVHRPTQVAGTAAVSVQKNEPGLGHFWTRQDGLR
                     LVLVAESETESESVNASFLCIDEFGTGLFKFQTATFDSPADVAALMASGPFKWIRGDA
                     DQTRKNLGVGLRILEAIGKEAPEGVAEFKEHLGPGSRLSLPPSIYACPVCDAALPPEI
                     VDAIKEVAKNGGDSYMACQRCTARRQLGAKRSNYVVPAHRRLKSAFQDSETLTLTPNG
                     WNILERAIPFDSLEEGVGLLLTQDVMKGILYAIERFIADATVPNPQLNDSHVVAALEH
                     LKHALEQNQELKAVDPFEVNFVLEALDEGMALVKQIFREEVETEPDPTLLLNAVEMVL
                     ASVRRRSARQPDKREYIQFICNYV"
     gene            2361..3092
                     /locus_tag="PKZ53_09345"
     CDS             2361..3092
                     /locus_tag="PKZ53_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ40682.1"
                     /translation="MRKVCVMCSKGGTGKSTTAVNVAAGVADQGKKVLLIDCDAQGNA
                     SAHLGAKAGPTIYDVLIGEASWDTAKQTIRTNLDLIPATISLSAVSVFLMQQKNPVNV
                     MRRRFGNLEGYDLVLLDCAPSFSTLHSNVLLFVEEAWIPVSMEYFALLGVAQLVNEIS
                     AIAEELETTVEIRHVIPTFFDSRNRKTLQIMDMLKEQFPNQLTGPIRTDVRLSEAPGF
                     SQSIFEFAPRSRGAEDYQRLISEVL"
BASE COUNT          784 a          773 c          834 g          747 t
ORIGIN      
        1 ggtgctttgt aatggaagaa agcatctcga tgcgctcgtc aacggtgaac aggcacaggg
       61 ggaattctac atcccgctgg caacatcgga aggcacgctg gttgccagct atagtcgcgg
      121 gatgaaagtg attaacctct caggtggcgc aacagtcacg gtcacggctg accaaatgca
      181 gcgggcgccg gtctttatct tccgcaatgc ccgcgaagcc agggattttg ccaattggat
      241 ccgagataac ctagatcaga tccgcaccgt ggcggaaagt acctcgcgga ttgccaaact
      301 cgtcagcatt gagttctatc agtctaataa attcctgtat acccgattta attattcaac
      361 cggggatgca gcgggccaga acatggtcgg tcgggccacc tatgcggctt caaaatggat
      421 ggttgaagcg cacggaggtg tcacgcgctg gtttttggaa tccaattttg ccaccgataa
      481 aaaaggctca caaattaact cgattatgac gcgtggaaag cgagtcaccg cggaatgtac
      541 cattccgcca gaggtgctca aacaggtgat gcgaacaacg ccagagcaac tccactacca
      601 ctatagcgtc gccaatgtgg ccgggttttt gtcaggcgtc aacaacaacg gggcccactc
      661 agccaatgcg attaccgcca tgtttatcgc cacgggccag gatgtcgcca acgtcgctga
      721 gtcctcagcc ggcgtcctgt attgtgaggt caccgacaat ggttcgctct atatttcgct
      781 cacgattcca tccttgatcg tggcaacgtt tggcggtgga acagggttgc caacccagca
      841 ggaatgtctg ggcatcctgg gatgtcaggg gagaggcaag gtgaataaac tggccgaaat
      901 cattgcagca gttgctctgg ctggggaaat ttcgctcagt tcagccatca gttcccacga
      961 ctgggtatca agccatgaac aatatggccg taatcggtaa ccctttcaaa caacatgaag
     1021 tggtggatat ttttaggaac tcccccttct catttttaat tggagtgata aagcaagcgt
     1081 catgccgaaa aagacaaaag cacaaaagat gaaagttcac cgtccaaccc aggtggctgg
     1141 aacggctgcg gtttctgttc aaaaaaatga accgggtctg ggacattttt ggactcgcca
     1201 ggatggcctg cgcctggtgc tggttgccga gtcagagact gaaagtgagt cggtcaatgc
     1261 aagcttcctg tgtattgatg agtttggaac tggcttattt aagtttcaaa ccgctacgtt
     1321 tgacagtcct gccgatgttg ccgcgttgat ggccagtggt ccgtttaaat ggatccgtgg
     1381 ggatgccgac cagactcgaa agaaccttgg ggttgggctc cgcattctgg aagcaatcgg
     1441 caaagaagcg ccggaaggag tggctgaatt caaggaacac cttgggccag ggtcacgcct
     1501 ctcactaccg cccagcattt atgcctgccc ggtgtgtgat gcggcactcc cgcccgaaat
     1561 cgttgatgcc atcaaagaag tggccaaaaa cgggggtgat agctacatgg cctgtcagcg
     1621 atgcaccgct cgtcgccagc ttggcgccaa acggtcaaac tatgtggtac cggctcaccg
     1681 acggctcaaa tcggcgtttc aggattctga aacattgacg ctgactccca acgggtggaa
     1741 tatcctcgaa cgtgctatcc cgtttgattc actggaagaa ggggttgggc tgctcctgac
     1801 ccaggacgtg atgaagggca ttctgtatgc catcgaacgc tttatcgccg atgcaacggt
     1861 accaaatccc caactcaatg acagtcatgt ggttgcagcc ttagagcacc tcaaacatgc
     1921 actggaacaa aatcaggaac tgaaagccgt tgatccgttt gaagtcaatt tcgttcttga
     1981 agccctggat gaaggaatgg cactggtgaa gcagattttc cgtgaggaag tcgagaccga
     2041 accagatcca actctgttgc tcaatgccgt ggaaatggta ttggcttcag ttcggcggcg
     2101 atcagcccga cagcctgata aacgtgagta cattcaattt atctgtaact acgtttgatt
     2161 tcccaatcaa cacactgttt aaatgaggga tccacctgag gaaactctgg cgtgggtccc
     2221 ttgtgtttga ggggtttcgg tagagagggg aaacccaatc ccgatcagcg gtctggttta
     2281 gaagaaagtt aatacgttaa aaggttttca cgaatcaacg ttttcccgtt ctttgtgaaa
     2341 cagttgcgga gaaaaggtcc atgcgtaaag tttgtgtgat gtgttccaaa ggcggaactg
     2401 ggaaaagtac gacggcagtc aatgtcgctg ccggcgtggc tgaccaggga aaaaaagtat
     2461 tgctcattga ctgtgacgcc cagggaaatg cgtcagctca cctgggtgcc aaggccggtc
     2521 cgacgattta tgatgtcctg attggtgaag ccagttggga tacagccaaa caaaccattc
     2581 gaaccaacct ggatttgatc ccggcaacca tttccctctc tgcggtttcg gtcttcttga
     2641 tgcaacaaaa aaatccggtc aatgtgatgc gccgaaggtt tgggaacctc gaaggatatg
     2701 acctggtgct gcttgattgc gcaccgagtt tttcaaccct gcattccaac gtgttgctgt
     2761 ttgtcgaaga agcctggatt ccggtcagca tggaatattt tgccctgttg ggtgtggctc
     2821 aactggtcaa tgagatttca gccattgctg aagaacttga aaccaccgtt gaaatccggc
     2881 acgtcattcc gacatttttt gatagccgga atcgaaaaac actccagatt atggatatgc
     2941 tcaaagagca atttcccaac cagttgactg gcccgatccg aactgatgtc cggctatcag
     3001 aagcccctgg ctttagccag tccatttttg aatttgcccc tcggtcgcga ggtgccgaag
     3061 attatcaacg cctgatttcg gaggttctgt aaaatggccc gcaataccaa agctccatcg
     3121 atgtccaatc cctttgcc
//
LOCUS       DAOQDO010000513         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24318_length_3129_cov_2.100520, whole genome shotgun sequence.
ACCESSION   DAOQDO010000513 DAOQDO010000000
VERSION     DAOQDO010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24318_length_3129_cov_2.100520"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKZ53_09350"
     CDS             complement(<1..1051)
                     /locus_tag="PKZ53_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40683.1"
                     /translation="MPRLSQLLRLTACCLALFSLVFSFTPPLTQAGSDCPECGDQRDR
                     GRNRDQDRDRDRDRDWQRNRDRYEDRYDNEATVYLAYRSVNQQRILVSSWDGGRWSYG
                     QEIKDALSDVAPDICSDGRQLYLAYKGRSSNKIFYRTSVSGFEWSRELEVKNAQSNDS
                     PSLAVYRGEVYLAYKGVNSPSIYIRKFDGRNWSSEEKVPNSESSTSPNIAACAGELYL
                     GYRGRNSAKIYLRAYAGGRWSTETQLPDARSANAPALSCYKKDLYVVYRGESGKSLFW
                     MKWDGRRWTDEKTIGSSETSQPPSLFRQGGYLYVAYKGGSSTNVYLRRFDGSSWGSEQ
                     KVPELESVDTPAAIGS"
     gene            1606..>3129
                     /locus_tag="PKZ53_09355"
     CDS             1606..>3129
                     /locus_tag="PKZ53_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNJ40684.1"
                     /translation="MDPNARRRFHAPGILTWGAMILVCLVIIFGWLMVTQSSENARQA
                     QFTREAEWTQTLINNRLLVYENLLNGASAFVSATPSVNRSTWRAYTDELELLERYTAI
                     GGLGFIQMVPEREVIPFLDRVKAEDAPFFKITPPSVQDQHYVLTYLEPISANFALIGF
                     DLASDPKCRKAIEQARDTGQFTISAPVMMPQSPSAQGTIFLLLPVYRRQSLLVTVEDR
                     RQALIGWVCAPLNLNELAQKLTQGVQTPVDFAIYEESGTDADTLLFSTLSEAELTQRH
                     QSKEVTQDLKLIAGKRWLVRVWPSARTTSATEQVYQGILIFGGVGIGLLVFFIFWLLA
                     TSRQQAIELAQSMTSELQKFSSLQKAILDSTSYTIISTDTQGIIQTFNQAAEKLLQYS
                     AAEVVGKITPALFHDSEEIKEKARQLTAESGRQIEPGFEVFIEKARSGIPDEQEWTYI
                     RKDGSRFPALLSITALHDPDGTITGYLGIGSDITRRQQTVETLKREQGQFQALIVNAP
                     "
BASE COUNT          774 a          805 c          787 g          763 t
ORIGIN      
        1 agctaccaat cgcggctggg gtatcaactg attccagttc gggcactttt tgctcacttc
       61 cccagcttga accgtcaaaa cgccggaggt aaacgttggt ggacgaaccg cctttatatg
      121 ccacatagag atagccaccc tgccggaaca ggcttggagg ttgcgaagtt tccgaactcc
      181 caatcgtttt ttcatccgtc cagcggcgac catcccattt catccaaaac agacttttcc
      241 ccgactcacc ccggtacacc acatagaggt cttttttgta acacgacagg gcgggggcat
      301 tggccgagcg ggcgtctggc aactgggttt cggttgacca gcgaccacca gcataggcac
      361 gcagatagat tttggcagaa ttgcgaccgc gataccccaa atacagttcg ccggcacagg
      421 cggcgatatt ggggctggtt gatgattcag aattggggac tttttcttcg ctcgaccagt
      481 ttcgtccatc aaatttgcga atgtaaattg aggggctatt gacgccttta taggccagat
      541 acacctcgcc gcgatagaca gccagacttg gcgaatcatt ggactgagcg tttttgactt
      601 cgagttcacg cgaccactca aacccgctga cgctggtccg gtaaaaaatc ttgtttgaac
      661 tccgcccttt gtaagccaga tacagttgcc ggccatcgct acaaatatca ggcgcgacat
      721 cgctgagggc gtccttgatt tcctgaccat aactccaccg gccaccatcc cagcttgaaa
      781 ccaggattcg ttgctggttg accgaacggt aggccagata gaccgtggct tcattatcat
      841 agcgatcctc ataccggtcg cgattgcgtt gccagtcgcg atcccggtct cggtcccgat
      901 cctggtcccg atttcgcccg cgatcacgct ggtcaccaca ctccggacaa tcagatccgg
      961 cctgtgtcag cgggggagta aagctaaaaa ccaaactgaa cagagcaagg caacatgccg
     1021 tcaatcgaag cagttgtgaa agtcgtggca taaatgtttt tctccgatca atgaaaaatt
     1081 gcaatgatgg aatccaggag caacgggcta ccgagagcgt agctcgcggc gacgcggaaa
     1141 aggtaatcgt tggtttttca aaatctttcc ggcgttggct gacaggttga attgatagct
     1201 gatttgcgtg agatcaagtg gaggttttgt ctggtaaggt aggccaaatc tggaaattgt
     1261 caatgttcct gcaaaattcg cagaagatgg tttcaggcga ggaatccagc gtgtaaaatg
     1321 cacctgctcg caggctcgcg ttctatccca ttcagattag tctacttaac atcattttct
     1381 tttcaattgc tgccagcttc attttgaagt tctcgtgaca ggcgcttcaa gattgaaggt
     1441 ctttgtgatg aggaaaatcc aggttctgtg tggctcaaac cagaatttcc ccatcaggag
     1501 tccagctagt aattccctcg ataacccaat gtttctagat agtttaggtg acattacaac
     1561 tttgagtaga agtccatttt catgagattt caccaatctt ccatcatgga cccaaacgcc
     1621 agacgtcgct ttcacgcccc agggatattg acctggggcg ccatgatttt ggtgtgcctg
     1681 gtgatcatct tcggctggct gatggttacc caatcctctg aaaacgcacg ccaggcccag
     1741 tttacacggg aggcagagtg gacccaaacg ctgattaaca accggctact ggtctatgaa
     1801 aatttactca acggtgcctc ggcctttgtg agtgcgaccc cgtcggtcaa ccgatcaacc
     1861 tggcgcgcct acacagatga actggaattg ctcgaacgct acaccgcaat tggtggattg
     1921 ggattcattc agatggttcc agagcgtgaa gtgatcccat ttcttgatcg ggtcaaagct
     1981 gaggacgcgc ctttttttaa gataacccca ccttcggtgc aggatcagca ttatgtcttg
     2041 acgtacctgg aaccaatcag cgccaatttt gccttgattg ggtttgatct ggccagtgat
     2101 cccaaatgcc gaaaagccat cgaacaggcc cgcgataccg ggcaatttac catctctgcc
     2161 ccagtgatga tgcctcagtc accgtcagcc cagggcacta tttttctgct gctgccggta
     2221 tatcggcggc aatcactttt ggtcacggtt gaagatcggc gacaggccct cattgggtgg
     2281 gtatgcgcgc cactgaacct gaatgaactg gctcaaaaac tgacgcaagg ggttcaaact
     2341 ccagttgatt ttgcaatcta tgaagaaagc ggcacggatg cggatacctt actgtttagc
     2401 accctgtctg aagccgaatt gacgcaacga catcaatcca aagaggtgac tcaggatttg
     2461 aagttgattg ctggaaaacg ctggctggtg cgggtctggc catccgccag aacaaccagt
     2521 gccacagagc aggtgtatca ggggattctg atttttggcg gagtcggtat tgggctactg
     2581 gtctttttta ttttctggtt gctggcaaca tcccgacaac aggcaattga actggctcag
     2641 tcaatgacca gtgaattgca aaaattttct tcgttgcaaa aagccattct ggatagtacc
     2701 agctacacga ttatttcaac tgatactcag ggcatcatcc agacctttaa ccaggcggct
     2761 gaaaagctgc tgcagtattc agccgcagaa gtagttggaa aaatcacacc agctcttttt
     2821 catgactcag aagagatcaa agagaaagcc agacagttga ccgccgaatc tggccgtcag
     2881 attgagccgg gctttgaggt atttattgaa aaggccaggt cgggaatccc cgatgaacag
     2941 gaatggacct atattcgcaa agatggcagc cgattcccgg cgttgttgtc aatcacggcg
     3001 ctccacgatc cagacggcac catcaccggg tatctgggca ttggaagcga catcacccga
     3061 cgtcagcaaa cggttgaaac cctcaaacgc gagcaggggc aatttcaagc gctcatcgtc
     3121 aatgccccg
//
LOCUS       DAOQDO010000514         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24343_length_3127_cov_2.584310, whole genome shotgun sequence.
ACCESSION   DAOQDO010000514 DAOQDO010000000
VERSION     DAOQDO010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24343_length_3127_cov_2.584310"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PKZ53_09360"
     CDS             <1..1747
                     /locus_tag="PKZ53_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ40685.1"
                     /translation="IEQAITYQSRGNEVSERDLVRNLVSGSERQKLLYLSRTAGNTDS
                     TLTLQVESAPQNLAALSAALTIVLRRKGRALDVMTSAIETLRRRQNPETQKLLDDYAS
                     LASQVSVLTLRGPGNRKPEDHLAYLRKLETQKEKLEAEISTRSAEFKTQVTPITLEAV
                     RKLIPPDATLLEYAVYRPYDAKTRQFGNARYVVYLLDQSGEIRFADLGETEPIQKAVA
                     AFRRAVSDQASTDAWSRKLSLLTQATGGNSTQANVKLAGHQLEKLIFEPVRTLIGTSK
                     HLLISPDGDLNLIPFAALTDETGKYLAENYLMTYLTSGRDLLRLQAKTSSQEPPFILV
                     NPAYGTGKGPVLFGNTYEPLSQLNGTTAEGTILKRLFPDATLHLDAQATKKTLQSVMG
                     PSIAHIATHGSFLQNLPQEPSPTSPEQRGLTLEDELRPVESEKLREANPLLRSCLFFA
                     GANTGGTSTEDDGKLTALEAAQLNLWGTKLVVLSACDSGVGDIKNGDGVYGLRRALVL
                     AGSESQVMSLWPVSDQGTKELMTNFYRRLKAGEGRSQALHNSQLDMLKSKRWKHPFYW
                     AAFIQSGDWTNLDRK"
     gene            1863..>3127
                     /locus_tag="PKZ53_09365"
     CDS             1863..>3127
                     /locus_tag="PKZ53_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair domain-containing protein"
                     /protein_id="HNJ40686.1"
                     /translation="MASDDSRTKLSTSREIISRILWDSRLNRAAFRVGYQDRLAPEGF
                     REKTVDEWAVASDIPWHRVRFVRCGETRVWDRETHLDLFATDELPAAAWVEPPERISA
                     PTSLLISETPSANPDFACVSRPVFQNSHFEWNAISTEVNPVSVDRLKIITFNILAQLP
                     DDGDLRTPERTTQLLLLLQSSQADVIALQEVTPTVLAALQTADWTAGWFLSEPILSPN
                     LDPHGVLVLSRYPFSQVDHWFSLRKRVLIATWTLNDQPLHIAVLHLPSSMTSEAPARR
                     RLYTGKLVEYLSQLPGDCAVVGDFNTPGDELDQLFQHEDFDDLWPRLNPTDPGLTYDP
                     VTNDLAAQLSRSGNSSRYDRMWVRSKNWRPTQIARLGVEPVATPSGPLFLSDHFGLDA
                     SFEQWKPQALNPEPRTLLQMTAPVYTSAIV"
BASE COUNT          719 a          880 c          832 g          696 t
ORIGIN      
        1 catcgaacaa gccatcacct accagtcgcg cggcaatgaa gtgagcgagc gcgatctggt
       61 tcgaaatctt gtttcgggct ccgaacgtca aaagctgctc tatctcagcc gaacggcagg
      121 caatacggat agcacactta ctcttcaggt ggagtcagcc ccacagaacc tggcggccct
      181 gtctgcggca ttgaccattg tattgcgtcg caaagggcgg gcactcgatg tgatgacctc
      241 ggcgattgag accttgcgac gccgacaaaa ccctgagacg caaaagttgc tggacgacta
      301 tgcgagtctg gccagccagg tctcggtgtt gaccttgcgc ggccctggta accggaagcc
      361 ggaagatcat ctggcctatc tccggaaatt ggaaactcaa aaagaaaaac tggaagccga
      421 aatcagtacc cggagcgccg aattcaaaac ccaggtgaca ccaatcacac tggaagccgt
      481 gagaaaattg attccacccg atgccactct tttggaatat gccgtctatc gtccgtatga
      541 cgcgaagacc cgccagtttg gaaatgctcg ctatgttgtc tacctgctcg accaaagcgg
      601 ggaaatccga tttgccgacc tgggtgaaac cgagccgatt cagaaagcgg tggccgcgtt
      661 tcgccgcgca gtgagcgatc aggcttcaac ggatgcctgg tcccgcaaac tcagcctttt
      721 gactcaggcg actggaggaa attcaactca agccaatgtc aaactggctg gacaccaact
      781 ggaaaagctg atttttgaac cggtcagaac gttgattggt acctccaaac acctgttgat
      841 ttcaccggat ggcgacctga acctgattcc ctttgcggct ttgaccgatg aaaccgggaa
      901 gtatctggcg gaaaactacc tgatgaccta tctgaccagt gggcgggatc tgctgcgact
      961 gcaagccaaa accagcagcc aggaaccacc ttttattctg gtcaacccgg cctatgggac
     1021 tggaaaaggg ccggtactgt ttgggaacac ctatgagccg ttgtctcaac ttaatggaac
     1081 caccgcagaa ggtactatcc tcaaacggct tttcccggat gccactctgc acctggatgc
     1141 ccaggctacg aaaaaaaccc tccaatccgt tatgggacct tcgatagcgc atattgccac
     1201 ccacggttct tttctccaaa acctgcctca agaaccatcg cccaccagcc ctgagcaacg
     1261 gggattgact cttgaagacg aattgcgccc ggtggagtct gaaaaactgc gggaggccaa
     1321 tccgctgctc cggtcgtgtc tcttttttgc gggcgccaac actggtggca ccagtaccga
     1381 agacgatgga aagttgacag cgctggaagc cgcgcaactc aacctgtggg gcaccaaact
     1441 ggtcgtgttg tcggcctgcg attcaggcgt gggcgatatc aaaaatggtg atggggtcta
     1501 tgggttacgc cgggcgctgg ttctggccgg aagtgagtca caggtgatga gcctgtggcc
     1561 agtgtcggat cagggcacca aggaattgat gacaaatttc taccgccgct tgaaagccgg
     1621 cgaaggccga agtcaggcac tgcataacag ccagttggac atgttgaaaa gcaagcggtg
     1681 gaaacaccct ttttactggg cggcattcat tcaatcgggt gactggacaa atctggacag
     1741 gaaatagcgc tccgttcagt cagtaccacc cgcatcagtg ggtgggccag cgtgcttcca
     1801 accccacccg ctcacgcagg tggtactgac aagtcctttt cattcttcat tcttcattct
     1861 tcatggcttc agacgactca cgcaccaaac tcagtaccag tcgcgaaatc atcagccgga
     1921 tcctgtggga ttcgcggctc aatcgggctg cgtttcgggt tggctaccag gatcggctgg
     1981 cacccgaagg ctttcgcgaa aaaaccgtgg atgagtgggc cgtggccagc gatattccct
     2041 ggcatcgggt gcgctttgtt cggtgtggtg agacccgagt gtgggaccgt gaaacacatc
     2101 tcgacctttt tgcaacggac gaactccccg ccgccgcctg ggttgagccg cctgagcgga
     2161 tttcagctcc aaccagtttg ctgatttctg aaacaccgtc ggctaatccg gattttgctt
     2221 gtgtctcccg acctgtgttt cagaacagtc atttcgaatg gaatgccatt tccaccgagg
     2281 tgaatccggt ttcggttgac cgcttgaaga tcattacctt caacattctg gcgcaacttc
     2341 ccgacgatgg ggatttgagg acaccagaac gcacaactca actgcttttg ttattgcaat
     2401 ccagtcaggc agacgtgatt gcgctccagg aagtcacccc cactgttttg gctgcgcttc
     2461 aaacggctga ctggactgcc ggctggtttc tttccgagcc gatactgtct ccgaatcttg
     2521 atccgcacgg ggtgctggtg ctgagccgat atccgttttc acaggttgac cactggtttt
     2581 cactccgcaa acgggtgctg attgccacct ggacgctcaa cgatcagcca ttacacattg
     2641 ctgttctgca tttgccaagc agtatgacaa gtgaggcccc ggcgcggcga cggctgtata
     2701 ccgggaaact ggtcgaatat ctcagtcagt tgcccggtga ttgtgccgtg gtcggagatt
     2761 tcaacacgcc cggtgacgaa ctggatcaac tgtttcaaca tgaagacttt gacgacctct
     2821 ggccgcgatt aaaccccact gacccagggc tgacctatga cccggtgacc aatgacctgg
     2881 cggcacaact ttcgcgctcg ggaaactcat cgcgctatga ccggatgtgg gttcggtcga
     2941 aaaactggcg ccccactcaa attgcaaggc ttggcgtcga gccagttgcc acaccctccg
     3001 gtccgctttt cctttccgat catttcgggc ttgatgcgag ctttgaacaa tggaaacctc
     3061 aagccctgaa tcctgaaccc cgaacccttt tacaaatgac cgctccggtc tataccagcg
     3121 cgattgt
//
LOCUS       DAOQDO010000515         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24373_length_3125_cov_3.014658, whole genome shotgun sequence.
ACCESSION   DAOQDO010000515 DAOQDO010000000
VERSION     DAOQDO010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24373_length_3125_cov_3.014658"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKZ53_09370"
     CDS             <1..447
                     /locus_tag="PKZ53_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNJ40687.1"
                     /translation="GNKKLVVGRVKPDKIAQFSQYRFWNGRTWVTTFRSAAPVASRLS
                     SEFSVTPLADGRYLLVFQLDTLSNTVAVSYGSSPTGPWGPPIPIWTCPETTLTPNITT
                     YNAKAHPHLSAPGELLISYNVNTFSFAEHFANADIYRPRFIRLPLP"
     gene            467..2407
                     /locus_tag="PKZ53_09375"
     CDS             467..2407
                     /locus_tag="PKZ53_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40688.1"
                     /translation="MKKLILFLALSLIPLLSQPALAGEKVTRLEIKVEPGTRIRPLET
                     AVIQVRVFGEVEKKDKEILTGRLKKTGAKVRVVSRDGGWISKEFKYQGTDDGPFVSSE
                     NRFFQVLNQATIQDCFLYSAPDRPGRYTIEASLDGKTATVVMDVSTSAQARKPAERQE
                     FRDERASRDSYFELAEHYAPFVAQETWFQPKADYITRFDYDGDWRGDNNWDSLDQGTS
                     QAYVYYTVMETESHWFVIYNLFHPRDYSDTCIAGTCHENDNEGLILTVAKDRSQFGRL
                     QAMETLAHNNIYSFRADSQVRNGFHDIDGEVELYQGSHPVIFIESGGHGVYGSQGKNS
                     RYSLSNDTFSAGTGVTFIYRGRAERPRHANDREVGYALLPIYEEWWERANSNAGREDR
                     TFDKYYAYQPFGNRPRPQMDQIAGSFYGRKEAADKAKPFWGWEDKATKKKNLLAVGQW
                     GLDPAYGAKTDLSFPDQSSFSLEYIYNPYLGIDRRSSASRWQSDRNERPTGSSRDSRD
                     NRRRSDDDWDDEPVSGPNDRFSSTDRDRDRDGRTNLNRKRCDLKFYVSGTDEVEIEVS
                     GSELYSPQERRRQFRNADSDFRDPLPRYEVSGFTVKQREGSGRVILVEKPGRANNYTA
                     RLRVTPASRSEEYYFVRLKWEE"
     gene            2472..>3125
                     /locus_tag="PKZ53_09380"
     CDS             2472..>3125
                     /locus_tag="PKZ53_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNJ40689.1"
                     /translation="MRHSIIVETFGGPEQLNLVEEASLPLGENAARVAIRAIGVNRAD
                     VLLRSGAYHGAQVPARPGIEASGEVVESTSEKFPVGSRVVIFSNRTGLYTTETVALEQ
                     HMALIPEGTSYEQAAALPINWLTAWYCIHSLVKLQAGETILIPAAVSGVGHAAIQISR
                     QIGARIIASASSDEKLAMAQQLGADVVINYTSQDLVSAVREATDGKGVNTFLDAVGGK
                     "
BASE COUNT          771 a          846 c          829 g          679 t
ORIGIN      
        1 ggtaataaaa agctggtggt tggacgcgtc aaaccagaca aaatcgccca attttcacaa
       61 tatcgtttct ggaatggccg aacctgggtc acgaccttcc gttcggcggc accggttgcc
      121 tcccgacttt cgtcagaatt tagcgtcacg ccactggcgg atggccgcta tctgctggtt
      181 tttcaactcg ataccctgag caatacagtg gcggtcagtt atggcagcag cccgaccggg
      241 ccgtgggggc caccaattcc aatctggacc tgcccggaaa cgacactcac ccccaacatc
      301 acaacctata atgccaaggc tcacccacat ctgtcagccc ccggtgagtt gcttatcagc
      361 tacaacgtca ataccttttc ctttgccgaa cactttgcca atgcggatat ttaccggcca
      421 cggtttatcc gcttaccact cccttaaaga agtgaagttt ttgtctatga agaaattgat
      481 cctgtttctt gctttgtcgc tgatcccatt gctctctcaa ccagcccttg ccggagaaaa
      541 agtgacccgg ctggaaatca aagtcgagcc tggaactcgc attcgcccac tggaaaccgc
      601 tgtgattcag gtacgggtct ttggtgaagt tgaaaaaaag gacaaggaaa tcctgaccgg
      661 gcggttgaaa aagactggcg ccaaagtgcg cgttgtcagc cgggatggcg gatggatttc
      721 gaaagaattt aaataccagg gcaccgatga tgggccgttt gtcagcagtg agaaccggtt
      781 ttttcaagta ctcaaccagg ccaccatcca ggattgcttt ctctatagcg caccggaccg
      841 acccggacgc tacaccatcg aagcctcact ggacgggaaa accgcgaccg tggtgatgga
      901 tgtcagtacg agcgcccagg ctcggaaacc agccgaacgc caggagtttc gtgatgagcg
      961 agcttcgcga gactcatatt ttgaactggc cgagcattat gccccgtttg tcgcccagga
     1021 aacctggttt caacccaaag ctgactacat cacccgcttt gactatgacg gcgactggcg
     1081 tggtgacaac aactgggatt cgctggacca gggcacgtca caggcatatg tctactacac
     1141 ggtgatggaa accgaaagcc actggtttgt gatctataac ctgtttcatc cgcgtgatta
     1201 ttcagacacg tgcattgccg gtacctgcca tgaaaacgac aacgaagggt taatcctgac
     1261 ggttgccaaa gatcgaagcc agtttggtcg ccttcaagcc atggaaacac tggcccacaa
     1321 caatatttat tcattccgcg ccgatagcca ggtccgcaat ggatttcacg atattgacgg
     1381 cgaagttgag ttgtatcagg ggtcacaccc ggtgattttt atcgaatccg gcggacatgg
     1441 tgtgtatggc agccagggca aaaattcgcg gtattcgctt tcgaatgaca cgttctcagc
     1501 cggaactggt gtaactttta tctatcgcgg acgggctgag cgccctcgtc atgccaatga
     1561 ccgtgaagtc gggtatgcct tgctcccaat ctatgaagag tggtgggaac gggcgaattc
     1621 caatgccggg cgcgaagatc gaacctttga taaatactat gcttatcagc cgtttggaaa
     1681 tcgtccacgg ccacagatgg atcaaattgc cgggtcgttt tatggacgca aagaagctgc
     1741 ggacaaagcc aaaccattct ggggctggga agacaaagcc accaagaaga aaaacctgct
     1801 tgccgttggc cagtggggac ttgatcccgc ctatggtgcc aaaactgacc tgagcttccc
     1861 agatcaatca tcattttcgc tggaatacat ttacaacccg taccttggaa tagatcgccg
     1921 gtcatcagct tcacgatggc agtctgatcg caacgagcgc ccaaccggat cttcaaggga
     1981 ttcaagagat aatcgccgcc gttcggatga tgattgggac gatgaaccag tttccggacc
     2041 caatgaccga ttttccagca ctgaccggga tcgagaccgc gatgggcgaa ccaacctcaa
     2101 ccggaaacgc tgtgatttga agttttatgt cagcgggacg gatgaagttg aaatcgaagt
     2161 ttccggaagc gaactctaca gcccccagga acgccgccgg caattccgca atgccgattc
     2221 tgattttcgc gacccgctgc cgcgatatga agtttcaggt tttacggtca agcaacgaga
     2281 aggctcaggc cgggtcattc tggtcgaaaa gccaggtcgg gcgaacaact acaccgcccg
     2341 gcttcgagtc acccccgcca gtcgcagcga agaatactat ttcgtgcggc tcaaatggga
     2401 agaataggac aaagcgctgt caagccagcg cactccacag cgccgtcaag ccggcgcact
     2461 ccacatcttt tatgcgtcat tcaatcattg tcgaaacatt tggtggtccg gaacaactca
     2521 accttgtgga agaagcctcg ctcccactcg gagaaaacgc ggctcgggtc gcaattcggg
     2581 cgattggcgt caatcgcgcg gatgtgctct tgcgaagcgg ggcgtatcac ggcgcccagg
     2641 tgccagccag acctggcatt gaagccagcg gtgaggttgt cgaatcaact tcggagaagt
     2701 ttccggttgg cagccgggtc gtgatttttt caaaccgaac gggcctctac acgacggaaa
     2761 ccgttgcatt ggaacaacat atggcgttga ttccagaagg aacttcctac gaacaggccg
     2821 ccgccctgcc gatcaactgg ctcaccgcct ggtattgcat ccattcgctg gtaaaactcc
     2881 aggccggaga gacaatcctg attccagccg ccgtgagcgg tgtcggacac gcagccattc
     2941 agatttcccg ccagatcgga gcgcgcatca ttgccagtgc gagcagcgat gaaaaactgg
     3001 cgatggcgca gcaacttggt gcagatgtgg tgattaacta cacgagtcag gatttggtga
     3061 gtgccgttcg tgaagccact gatggaaaag gcgtcaacac attcctcgac gcggttggcg
     3121 gcaaa
//
LOCUS       DAOQDO010000516         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24456_length_3118_cov_3.368919, whole genome shotgun sequence.
ACCESSION   DAOQDO010000516 DAOQDO010000000
VERSION     DAOQDO010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24456_length_3118_cov_3.368919"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1429)
                     /locus_tag="PKZ53_09385"
     CDS             complement(98..1429)
                     /locus_tag="PKZ53_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ40690.1"
                     /translation="MSSQSSLWLFFNRIIGALAIDVTPLKASRDYRLLYTGQFVSTFG
                     TAISYVVLPLQVYQLTHSSFAVGMLGVAEFVPMLSLALVGGALADAVNRQKLLIVTEI
                     GLAAGCGALVYNAVLPQPHLWVLYVAAAFFASLSALHRPAHEALTPRVVAPEHLPAVS
                     VLNTLKYNFGHIAGPALAGIIAASFGPAIAFAIDGATYLVSLTTVMLMRAVPAPADAD
                     RPSLGSIVEGIKYARSRQELLGTYLIDINAMFFGMPFALFPAIAQQFGNASVGLFYAA
                     PSVGALLAGLTSGWTERIHRHGLAVTIAASVWGLAIVGFGLSNSLWLALLFLGLAGAA
                     DNISGIFRMTIWNQTIPDYLRGRMAGIEMVSYLSGPYLGNAEAGLVASLLSLRTSVVS
                     GGVLCVAGSAVLAVLLPKFISYDGRTGVAHKLEAETARNNAITAKLQAQEE"
     gene            1907..2260
                     /locus_tag="PKZ53_09390"
     CDS             1907..2260
                     /locus_tag="PKZ53_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40691.1"
                     /translation="MKALTQPQTPSIASLAGLVWKWALVGFVFGGLLGGGIIFRACAG
                     YVHRGKVCSPCIIQSPGFSPNLSLTTIYVLGIATGGLLGGIIGGIFKMSAVSPCPNSL
                     PTKKQTIISGITYLP"
     gene            2378..>3118
                     /locus_tag="PKZ53_09395"
     CDS             2378..>3118
                     /locus_tag="PKZ53_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ40692.1"
                     /translation="MYTSLKPEKHNHSHAPNKFSVLPTPVRLNALFEYTGKGVTIAFL
                     DSGFYPHPDLTMPTNRILAYVDITNPEATLDPDAEPEGIAWHGTQTCVAAVGNGYYSD
                     GAYRGVASDARVVLIKVSQDGKIPEENIAKGIRWVIDNKEKYNIRVVNISLGGDEDVS
                     YKENIVDQAAEEAVSKGLVLVVAAGNSGDSAAHFTVPPANSPSVITVGGSDDKNRFGN
                     KTVDLYHSNWGPTADGILKPEIVAPAIWV"
BASE COUNT          786 a          817 c          801 g          714 t
ORIGIN      
        1 aaaccaattc ttcagcccga cttcttcagc ccgacttctt cagcccgatg tcttccgccc
       61 gatgtcttcc gcccgatgtc ttcagcccga agatttttca ttcttcctgc gcctgaagtt
      121 tagcggtaat ggcattgttg cgggccgttt cggcttcgag tttgtgagcg acacccgtgc
      181 gtccatcata gctgataaat ttggggagca gcaccgccag cacggctgaa ccagcaacac
      241 ataaaacccc accagaaacc accgaagttc gtaaactcaa cagacttgcg acaagacctg
      301 cctctgcatt gcccagatag gggccactca aataactcac catttcaatt cctgccattc
      361 gtccgcgcaa ataatctgga attgtctgat tccagatcgt cattcgaaaa ataccactga
      421 tattgtcggc agctccggca agtccaagga acaggagcgc cagccagaga ctgttggaaa
      481 gcccaaatcc aacaatggcc agtccccaga cagaagcagc aatcgtgacg gccagaccgt
      541 gacggtggat gcgttcggtc cagccggatg tgagtcctgc cagaagtgcc ccaaccgacg
      601 gtgcagcgta aaataatcca acggacgcat ttccaaactg ctgggcaatg gctggaaaca
      661 aggcgaaggg catgccgaaa aacatggcgt tgatgtctat caggtaggtg cccagtaatt
      721 cctgccggct gcgggcatat ttgatgcctt cgacaattga gcccagactc ggacgatctg
      781 cgtcagccgg ggcgggaacc gctcgcatca acatcacggt ggtgagtgaa accaggtacg
      841 tcgctccgtc aatcgcaaag gcgatggccg gaccaaaact ggccgcgatg atgcctgcca
      901 gcgccgggcc tgcaatatgg ccgaagttgt atttcaacgt gttgaggact gaaacagccg
      961 gaaggtgctc gggtgcgaca acgcgaggtg tcagggcttc gtgggctggc cgatggagcg
     1021 cgctcaggct ggcaaaaaag gcagcggcca catagagcac ccagagatgt ggttggggaa
     1081 gtacggcatt atagaccagc gcgccacacc ctgctgccag cccgatctca gttacaatca
     1141 agagtttctg gcgattgacc gcatcagcca gtgcgccacc aaccagtgcc aggctcagca
     1201 ttggaacaaa ttcagccacc ccgagcattc ccaccgcaaa tgatgaatgc gtcaactgat
     1261 atacctgcag cgggagcact acataactga tagctgttcc aaaagttgac acaaattgcc
     1321 cggtgtagag cagccgataa tcgcgagaag ctttgagcgg agttacatca atcgccagtg
     1381 cgccaatgat ccgattgaaa aaaagccaga gtgaagattg ggaactcata ttcttttggg
     1441 gttgagggat cacaacttag ggttcagggt tcagggttca ggggttgaaa ttagttggaa
     1501 gtgattgttt ctcaaccggt tgacattttt ctgtcaaaag aaatgattcc gctctggaat
     1561 gataaaggcg aaattgtcgg ttcagaattt gggtgtgaag ttggcacaaa cgccgtgaac
     1621 tgaaaaccag ttcctccgaa acctggaaag cctgtcgcat ctgtggcaga aaatgagtat
     1681 tctggtttct catccagcat ttgaagccag ccctgtctgc attggtgagt aggtatcgaa
     1741 atccaatgtc cgactttttc aactccattg cttttgatca acccctggtg cccgtatgtt
     1801 gagattccac atcaaagctg aaaaaccacc tgtgcgttgt gagatttgcc accagtcaga
     1861 ttgccttgac ccgatacaag atcaatgcct gcgttgttta gaaactgtga aagcattaac
     1921 tcagccccaa acgccttcca ttgcgagtct ggctggactg gtctggaagt gggctctggt
     1981 tgggtttgtg ttcggcggac tgctcggcgg cggaatcatc tttcgagcct gcgctggtta
     2041 cgttcataga ggcaaagtgt gcagtccttg tattatccag tcacctggct tttcacctaa
     2101 cctttcactg acaacgatat atgtgctcgg aattgcaact ggtggtttgc tgggagggat
     2161 cattggcggg atttttaaga tgagtgccgt gtcgccttgt ccgaattcat tacccacaaa
     2221 gaaacaaact ataatctctg gaattaccta cttgccataa tgcacaaccc tcaggccgta
     2281 aacgacctgc ctcaattcag ccatcgctac acggcgaatt ttcagaccct gaaccctgaa
     2341 ccctgaaccc tgaaccctga accctgaccc ctgatttatg tacacatcac tcaaacctga
     2401 aaagcacaac cattcgcatg ctccaaacaa attttcggtg ctcccgacgc cggtccggtt
     2461 aaatgcactg tttgagtata ccggcaaagg cgtcacgatt gcctttttgg attcagggtt
     2521 ttacccgcat cctgatttga cgatgccgac caatcggatt ctggcctatg tggacattac
     2581 caacccggag gccacgcttg acccggatgc tgaacccgag ggcattgcct ggcacggcac
     2641 ccaaacctgt gtcgcggcag tcggcaatgg atactactct gacggcgcct atcgcggcgt
     2701 ggcttcagat gccagagtgg tgctcatcaa agtcagccag gatggaaaaa ttcctgaaga
     2761 aaacatcgcc aaaggtattc gctgggtgat tgacaacaaa gagaaataca acatccgtgt
     2821 ggtcaatatt tcgcttggcg gcgatgaaga cgtttcctac aaagaaaata tcgttgacca
     2881 ggcggctgaa gaagctgtga gtaaaggact tgtacttgtg gtggcagccg ggaattcagg
     2941 ggattccgct gctcatttca ccgtccctcc ggccaattcg ccctcggtga ttacggttgg
     3001 cggctcggac gacaaaaatc gctttggaaa taaaactgtt gacctgtatc actccaactg
     3061 ggggccgacg gcagatggca ttctcaaacc tgaaattgtg gcgccggcga tttgggtg
//
LOCUS       DAOQDO010000517         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24530_length_3113_cov_2.958470, whole genome shotgun sequence.
ACCESSION   DAOQDO010000517 DAOQDO010000000
VERSION     DAOQDO010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24530_length_3113_cov_2.958470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="PKZ53_09400"
     CDS             complement(<1..1456)
                     /locus_tag="PKZ53_09400"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNJ40693.1"
                     /translation="MVALCTPDSVYWCDGSEEEYDRLCEQMVQAGTFKRLNQEKRPNS
                     FLALSDPSDVARVEDRTFICSSRKADAGPNNNWADPKEMKATLNGLFSGSMKGRTMYV
                     IPFSMGPLGSPMAQIGIEITDSPYVVVNMKIMTRMGRKVLDILGDGSFVPCLHSVGMP
                     LEPGQADISWPCNPTVKYIVHFPEERSIWSYGSGYGGNALLGKKCLALRIASVMGRDE
                     GWLAEHMLILGVEAPDGEKTYVAAAFPSACGKTNFAMLVPPKGFEGWKVTTVGDDIAW
                     IKPGPDGKLYAINPEAGYFGVAPGTSVKSNPNAMASIEKNTIFTNVALTPDGDVWWED
                     MTDEPPASCIDWQGNEWTPGCGRKAAHPNSRFTAPARQCPSIDKNWESPNGVPISALI
                     FGGRRATAIPLVYQAVNWNFGVYLAATVGSEMTAAAFGNIGQVRRDPMAMLPFCGYHM
                     GDYFNHWLQFGRDLPTPPRIFCVNWFRKDADGKFL"
     gene            complement(2664..>3113)
                     /locus_tag="PKZ53_09405"
     CDS             complement(2664..>3113)
                     /locus_tag="PKZ53_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ40694.1"
                     /translation="VNALDYLLKPIHPTRLERAIERLTNPEAIRTASPVRPLTYDDRL
                     FLEVDGQSRFLKVNAIVCIQAASDYSEIFTLDGRRLMVLKPLREWEERLPEQYFARIH
                     RSAIINLEFVEHIENWSHRACRVFLRNMAEPLVMSRRYVTRLKLKFG"
BASE COUNT          809 a          799 c          738 g          767 t
ORIGIN      
        1 agaggaactt gccgtcggcg tctttgcgga accagttgac acagaaaatg cgtggcgggg
       61 ttggcaggtc gcggccgaat tgcagccagt ggttgaagta atcgcccatg tgatagccac
      121 agaacggcaa catcgccatt ggatcacgac gaacctgccc aatgtttcca aaggctgcgg
      181 ctgtcatttc ggaaccaacg gttgcggcga gatacacgcc aaagttccag tttacagctt
      241 gatacaccag tggaatcgcc gttgcacgcc gcccgccgaa aattaaggca ctgatgggta
      301 cgccattggg actttcccaa tttttgtcaa tagatggaca ctgtctggct ggagcggtga
      361 aacgcgaatt tggatgcgcg gccttgcgcc cgcaacctgg tgtccattca tttccttgcc
      421 agtcaatgca gcttgcgggt ggctcatcgg tcatgtcttc ccaccacaca tcaccatctg
      481 gggtcagcgc cacattggta aagatggtgt ttttttcaat cgaagccatg gcattcggat
      541 tggacttgac cgaagtgcct ggggccacac caaagtaccc ggcttcaggg ttgatggcat
      601 acaacttgcc gtctggaccc ggtttgatcc aggcaatatc atcgcccacc gtcgtcactt
      661 tccagccttc aaaccccttg ggcggcacca acatggcaaa attggttttt ccacaggcgc
      721 tggggaaggc agcggcaaca taggtttttt caccatctgg cgcttcgacc cccagaatca
      781 gcatatgctc ggccagccag ccttcatcgc gtcccatgac ggaggcaatg cgtaaggcca
      841 gacatttttt gccaagcaag gcatttccgc cgtagccgct cccgtaggac cagattgagc
      901 gttcttctgg gaaatgaacg atatatttca cggtcggatt gcacggccac gaaatgtcag
      961 cctgtcccgg ctctaacggc atcccaactg agtgcagaca ggggacaaaa ctgccatcac
     1021 ctaaaatatc gagcactttg cggcccatgc gggtcataat cttcatattg accactacat
     1081 agggcgaatc ggtgatttca atcccgattt gagccattgg tgaaccaagc ggtcccatgc
     1141 tgaacgggat cacatacatg gtgcgtccct tcattgaacc tgaaaacaag ccgtttaagg
     1201 ttgctttcat ttccttcggg tccgcccagt tgttgtttgg accggcatcg gccttgcggg
     1261 agctacaaat aaacgtccgg tcttcaaccc gggccacatc gctcggatct gaaagggcca
     1321 gaaaactgtt gggtcgtttt tcctggttca atcgcttaaa agtacccgcc tggaccatct
     1381 gttcacagag ccggtcatat tcttcttctg aaccgtcgca ccagtacacc gaatcgggcg
     1441 tacacagcgc caccattttt tcgacccagg ctttgagctt gggattggtc acatagtctg
     1501 gaatcactga cgaaggttgg ggtgattcga attgagtttt ggtctccaca agaaaattcc
     1561 tccttgttcc aataccagga aaggacacct cgccccttcc gagaggcaac cggataagtt
     1621 caagttaata tgatgaagtg aaagtgaatg atgagtaagc atctttgaga atcttgtgag
     1681 ggattcacaa gtcagtggga aattatcacc attcgaaaaa gaaagaaacc caaatctttg
     1741 cgaaacagcc tccacacatg acaccacgtg taatggaaag catcggttac tgacatagag
     1801 gctatcccct ttggtcatca aacttccaaa cagaggattc aattccattt ccattggctg
     1861 agatgctttc tggtttactg aacgtgtcgc ttttggcgcc gggttcaggg gtgaaacaac
     1921 tgcttgtttt gcaacaaaaa tcgaacgtcg cacgccggtg cgatgagttc acaatgagaa
     1981 cctgtactcg ctgaaaccat catttccatc gagccagaag ttcctgacca gatgcaacag
     2041 accctggggg atccagtctc tggttacatc tctttttatg aactatgcaa atttcaagcc
     2101 accgaaccct ggatccagaa aaaaccagag gtatcagttc atattgagac atatgaaaca
     2161 atttgaggca atgaactgca acagagtaat ttaagtacct gaccgattca aggtgaccga
     2221 ttttttctaa ataaatgata aataacagat agacaagtac ttataggtaa taatcttatc
     2281 ttttaaagtt ctggcacgct gtttgaatat atctaagcca tcgagcatgt ggttttcctc
     2341 ctctcgggaa acagcagaaa aacacagcac ggacgcggat tacttggggt agaacaggac
     2401 agcaaccttt gaggtgcgta gaaatattcc gcgtccgttc ttcttttctt cgcaataatt
     2461 ttatcaattc aggtcttatg acgttttttt ccccaccccc taaatccaat ctgactgcct
     2521 gcctccgtaa gaggcagcat tgcaaggttc gcttaccttt atcgaacgca tagcttcttg
     2581 aaaagcattt cggcccagaa catccgtcat ccagatcaac atgacctgag tgacgaaggt
     2641 tctgggcctt tttttgtctt catctatcca aatttcaatt tcagtcgggt cacatagcga
     2701 cggctcatca caagcggctc agccatattc cgcaaaaaaa cgcgacaggc ccgatgtgac
     2761 cagttctcga tgtgttcaac aaactccagg ttgataatcg ctgagcgatg aatgcgggca
     2821 aaatattgct caggaaggcg ttcttcccat tcacgcaacg gtttcaacac catcagccga
     2881 cgcccatcaa gagtaaaaat ttcagaataa tccgaggccg cctgaataca cacaatggcg
     2941 ttgactttga gaaaccggga ctgaccatca acttccagaa acaagcggtc atcgtaggtt
     3001 agtggacgca ccggagaggc cgttcgtatc gcttcgggat tggtcaaccg ctcaatggct
     3061 cgctccagcc gggttggatg aatcggcttg agcagataat caagggcatt gac
//
LOCUS       DAOQDO010000518         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24570_length_3110_cov_2.844190, whole genome shotgun sequence.
ACCESSION   DAOQDO010000518 DAOQDO010000000
VERSION     DAOQDO010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24570_length_3110_cov_2.844190"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="argH"
                     /locus_tag="PKZ53_09410"
     CDS             complement(<1..816)
                     /gene="argH"
                     /locus_tag="PKZ53_09410"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNJ40695.1"
                     /translation="MTEQVLRGAFDKPIDELILQFVASIECDKALIEVDITGSLAHVW
                     MLEKTGVLSTEQAVKIRNGLLQILDEGIELKLEHEDVHMNIEKRLQELIGDDAKLLHT
                     ARSRNDQVALDLRLFVAQAQTDLISGLKALCQALVAQARKHHDVIMPGYTHLQRAQPV
                     LLAHVLLSFAEGLTRDITRLQPNLISPLGAGALAGTAIPINPGISAQLLGAKGHFANS
                     IDAVSDRDFAVEFVFACSLTAVHLSQLAENLILWCTSEFGFIKLPDELTTGSSI"
     gene            complement(1050..2240)
                     /locus_tag="PKZ53_09415"
     CDS             complement(1050..2240)
                     /locus_tag="PKZ53_09415"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNJ40696.1"
                     /translation="MEKIVLAYSGGLDTSVILTWLKETYNVPVVAYVGDVGQDEDLAA
                     VEAKAYRTGAESVVVLDLKDEFVAEYVFPAIQANAVYEGQYLMGTSLARPVIAKGMVQ
                     AALNSKASAIAHGATGKGNDQVRFELTVKALAPQLQIIAPWRDWSFVGRKDLLAYAAQ
                     KGIPVPVTAEKPYSMDANLLHISYEGGILEDPWAEPPADMFLWTKSPEQAPNKAETVE
                     IEFHKGIPVAINGEGYGPAKLLKVANDIAALHGVGRVDLVENRFIGIKSRGVYETPGA
                     TMLMQAHRAIESLTLDREVAHFKDALVPKFAELVYNGFWFSPEMELLLSTITKTQESV
                     TGVVRLKLYKGNVTVTGRKSPYSLYNPEVASFESKFVVNPKDSGGFININGLRLTTWA
                     SRRR"
     gene            complement(2240..2707)
                     /gene="argR"
                     /locus_tag="PKZ53_09420"
     CDS             complement(2240..2707)
                     /gene="argR"
                     /locus_tag="PKZ53_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNJ40697.1"
                     /translation="MKKPQDKTERQRLILTIITEHRIATQTELRDALREYGIACDQAT
                     VSRDIRELGLVRVADADGNTFYASLETAAPKRSGRIEILRQFLRSVQWSGNLLVIHTD
                     SANAHPVAEALDTLGFTEILGTVAGDNTIIAVVKEGTNAQALAERILKEAGKL"
BASE COUNT          685 a          822 c          829 g          774 t
ORIGIN      
        1 aatgctcgac ccggtggtta attcatcagg gagcttgata aagccaaatt ccgaggtaca
       61 ccacaaaatc aggttttcag ccagttgcga cagatgtacc gccgttaatg aacaggcaaa
      121 aacaaattcc accgcgaaat cacggtctga aactgcatca attgaattgg cgaaatgtcc
      181 tttggcgcca agcagttgcg cgctaattcc aggattgatc gggatcgctg ttcccgccag
      241 tgccccggcg cccagcggcg aaatcaaatt gggctgcagc cgggtgatgt cacgcgtcag
      301 cccttcggca aatgacagca gcacgtgcgc gagcaatact ggctgggctc tttgcaaatg
      361 cgtgtaccct ggcatgatga cgtcgtggtg cttccgcgcc tgggccacca gcgcctgaca
      421 cagtgctttc aagccagaaa tcagatcggt ttgcgcctgg gcgacgaaga gccgcaaatc
      481 caatgccacc tgatcgttgc ggctgcgcgc cgtgtggagc aattttgcat catcgccaat
      541 caactcctga agccgctttt caatgttcat gtggacatct tcgtgctcaa gcttgagttc
      601 aatgccttca tcaagaattt gaagcagccc gttgcggatt ttcacggcct gttccgtcga
      661 aagaacacca gttttttcga gcatccagac gtgagcgagc gaaccagtga tgtccacttc
      721 gatcagtgct ttatcgcact caatcgaagc cacaaactgc aaaatcaatt cgtcaatggg
      781 cttgtcaaat gccccacgga gtacttgttc ggtcatgatt ttagggttcc gggttccggg
      841 ttccgggttc cgggttccgg tattttaagg atgatttgtt tactcaggtc gcgtccaaat
      901 gaggtaaagt ttgtaccgac tttgtcagcg agcgggtttg gtttcctcaa acccggaacc
      961 cggaacccgg aacccggagc agaaagaaat atcccaggtc caaatgctga cacactcaca
     1021 gcaccgacct gggacgaagg gaaactaaat tatcgtcggc gcgaagccca cgtcgtcaag
     1081 cgcaatccat tgatgttgat aaagccgccg gaatctttcg gattcacgac aaacttgctt
     1141 tcaaaactgg cgacttcggg gttatagagc gaatagggcg acttgcgacc tgtcacggtc
     1201 acatttcctt tgtacagctt gagccggaca acgcccgtca cactttcctg ggttttggta
     1261 atggtggaaa gtaacagttc catttccggc gaaaaccaga atccgttata gaccagttcg
     1321 gcaaacttcg gcaccagcgc atctttgaag tgcgcgactt cgcgatccag cgtgagcgat
     1381 tcaattgccc gatgggcctg catcagcatc gtagcgcccg gagtttcata cacgccgcgt
     1441 gatttgatcc cgatgaagcg gttttcgacc agatccacgc ggccaacgcc gtggagcgcg
     1501 gcaatgtcgt tggcaacttt cagcaatttg gccgggccgt agccttcacc attgatcgcg
     1561 actggaatgc ctttgtgaaa ttcgatttcg accgtttcag ctttgttcgg ggcctgttcg
     1621 ggcgatttgg tccagaggaa catatctgcc ggaggctcag cccaggggtc ttcgaggata
     1681 ccgccttcat agctgatgtg cagcaggttg gcgtccatcg agtagggttt ttccgccgtc
     1741 accggcaccg gaatgccttt ttgtgctgcg tacgccagca aatccttgcg gcccacaaat
     1801 gaccagtcgc gccacggtgc aataatttgc aactgtggcg ccagggcttt gaccgtcagt
     1861 tcaaaccgca cctgatcgtt gcctttgccg gtggcgccgt gggcaatggc ggatgctttg
     1921 ctgttgagcg cggcctgcac cattcctttg gcaatcactg ggcgcgccag cgacgtgccc
     1981 atcaggtact ggccttcata gacggcattt gcctggatag ccggaaagac atattcggcg
     2041 acgaactcgt ctttgaggtc gagcacgacg accgattccg cgccggtccg gtaggctttg
     2101 gcttcgacag cggctaaatc ttcatcttga ccgacatcgc cgacataggc gacgaccggc
     2161 acgttgtagg tttccttgag ccacgtcaaa atgacgctcg tatcaagtcc cccactgtaa
     2221 gcaagtacaa tcttttccac tacagtttcc ctgcctcttt caaaattcgt tcggcgagcg
     2281 cctgagcatt cgtgccttcc ttcaccaccg cgatgatggt gttgtctccg gcgacggtgc
     2341 ccaggatctc ggtaaatcca agcgtatcaa gtgcctccgc gaccgggtgc gcattggctg
     2401 aatcggtatg aatcaccaga agatttccag accactgaac gctccgcagg aactggcgca
     2461 gaatctcgat gcgtcctgag cgttttgggg cagcggtttc gagcgatgca taaaaagtgt
     2521 tcccgtctgc atccgcgacc ctgaccagtc ccagttcccg aatatcgcgg gagactgtcg
     2581 cctggtcgca ggcaatgccg tattcgcgca gggcatcgcg aagctccgtc tgggtcgcga
     2641 tgcggtgttc agtgatgatt gtcaaaatca agcgttgacg ttctgtcttg tcttgaggtt
     2701 ttttcatatt tatgcagagt ttatgcaatt ttatgcacgt gtcaagatat tttttgggtg
     2761 agttcggaaa aaggcagttt cgaaagaatt tggagtgctg tgacttgtca cagctttggt
     2821 attgggcagc ttgttgccgg ttgacttgag ttgtgtggta acaggttacc gggatgaaag
     2881 cagcgacagg gtggcgcact ccaaaaagat agattggttg gttggtattt tgttccagat
     2941 atggctgaaa attcaatttt ttcaatgggt tgcttgtcct cgaataatcc tgatttcagc
     3001 ctcaattgtt tctgcctccg tcttacgccc catttcgcgg agcaaaacgg cataattttc
     3061 cattcctgtc agggtcacaa cgataaaccg gcggttgtgc tcaatacagc
//
LOCUS       DAOQDO010000519         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24579_length_3109_cov_3.675835, whole genome shotgun sequence.
ACCESSION   DAOQDO010000519 DAOQDO010000000
VERSION     DAOQDO010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24579_length_3109_cov_3.675835"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            612..>3109
                     /locus_tag="PKZ53_09425"
     CDS             612..>3109
                     /locus_tag="PKZ53_09425"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HNJ40698.1"
                     /translation="MEHKETEKKPSITELLQAHRRERESLHWEGKFREYFEMVVQNPK
                     LSQLAHARICEMILSAGVTKLNEGTRDEVTRYNFFSEELFGIDEAIEQLVEYFKSAAQ
                     RLEVRKRILLLMGPVGGGKSTIVTLLKRGLEKWTHTEPGAVYAIKGCPMHGDPLHLIP
                     ADLRKVIDKHYGIYIEGELSPQARYDLEHTYGGKHEDVLIERIVYSEKDRIGIGTFAP
                     SDPKCVVGNTILLTDQGMFRFDELQSRLQAEEDQFVPFNLTVSGRAGRETTSHFYNGG
                     IKPTRKIKTRLGFELEGSLVHPVLVLKDGEARWTKLADLNVGDCVALQRGQQMFGSMT
                     ALPAFEYAGPSSRGQNKNLTLPTELTSDLARLLGYIVAEGSLTDTGLWITNATPRVLD
                     DIRDICRQLFSVEPKTCTKAGTPAVSLSISSVKLVQWLETVCGITRGAASKRTPQIIS
                     SAPKELMLAFFEGLFWGDGTISARGRLGSNRFKLATASRVLATQLQVALLNMGIVAAV
                     HEERIKGKFTAYSVIVTGDAVVDLIELIPSLRDKCTDDPSRLALKRGRTNFNVIPEMH
                     SAVHALMQEARRQGQSLSTFRRYATTAAWGRNLTPQSLARLIEESEWLLGDASPALEL
                     LRERRDENLLWLEVQSITEGHAHVYDLTVPGTHSFCANGFINHNSQDISELTGSIDLS
                     SIGEVGVESDPRAYRFDGELNIANRGIMEFIEILKCIRGNSLIRTGLGVIQFDELWGN
                     MWGEKPPHPQDVRYQIGVQTRNGIEETTHLIYRGEGESLKIRTRKGYVLEGAPDHRIL
                     VLSIRGQFEWRALKDLEPEDRVCIDRKGYWTEQLP"
BASE COUNT          760 a          801 c          825 g          723 t
ORIGIN      
        1 gaatgaagaa ttgagaatga agaatgaaga attgagaatg aagaatgaag aatgagcctg
       61 gggcttgggg atttttcgaa agatttattc ttcagcccaa tttcttcagc ccgaagtctt
      121 cagccccgag cttgcgagtc ttcagcccaa tttcttcagc ccaacttctt cagcccgaag
      181 tcttcagccc gaagtcttca gcccggcttc ttcaaccctg tgtctggttg acgtcggctt
      241 gaattgagac gataattttt gcattcgaaa tttgcagaaa agttcaattt gttggctgga
      301 gatgcgagcc tcaggcagcc tttgcaccgt gtgtctgaat catgccaccg gtttttcggg
      361 ctaaagactg gcgaactgag cgctgaaagc acgggctgat gttacttttt atctcaccag
      421 gttacctcaa atccaatcgg acccagcccg atttcttcag cccccagccc gatttcctca
      481 gccccaaaag gctttaacca tcgcacaata tccgttgaaa aggtcattca caatcctgaa
      541 tttggtgaca aacgccaatt gtttgacttc actgtccaat ttttcgggca gcggaaaaga
      601 ggtggtcagt tatggaacac aaagaaactg aaaagaaacc cagcatcacc gaactgttgc
      661 aggctcaccg tcgcgaacgc gaatcactgc attgggaagg gaagtttcgc gagtactttg
      721 aaatggtggt gcaaaatccc aaactttcgc aattggctca cgcccggatt tgcgagatga
      781 tcctgtctgc cggggtcaca aagctcaacg aaggcacccg agacgaagtc acacgctata
      841 acttctttag cgaagaactc tttggcattg acgaagcgat tgagcaactg gtcgagtatt
      901 tcaaatcggc agcccagcgg ctcgaagtcc gcaagcgcat tctgttgctg atgggcccgg
      961 tcggcggagg caaatcaacg attgtgacgc tgctcaaacg cgggcttgaa aaatggacac
     1021 acaccgaacc tggtgccgtc tatgccatta aaggctgccc gatgcatggt gacccactgc
     1081 acctgattcc agccgacctc cggaaagtca ttgacaagca ttatggcatc tacattgaag
     1141 gcgaactcag cccacaggcc cgctatgacc tggagcacac ctatggcggg aaacacgaag
     1201 atgtgctgat cgaacgcatt gtctattctg aaaaggaccg gattgggatt gggacctttg
     1261 ctccgtcaga cccgaaatgt gtggtgggca acacgatttt gctgactgat caaggcatgt
     1321 tccgttttga cgaactccaa tcccggcttc aagccgagga agaccagttt gtaccgttca
     1381 atctgacggt cagtggtcgg gctggtcgtg aaactacgtc acatttctac aatggcggta
     1441 tcaagccgac ccgaaaaatc aaaacccgac ttggttttga actcgaaggc tcgctggtcc
     1501 atccagtgct ggtcttaaaa gacggagagg ctcgctggac caaactggct gatctgaacg
     1561 tcggtgattg tgtggcttta cagcgcggcc agcagatgtt tggttcgatg acggcgctcc
     1621 cggcttttga gtacgccggg ccctccagtc gtggccagaa caaaaactta accctgccga
     1681 ctgaactgac ctccgatctg gcgcgcttgc tgggctacat tgtggctgaa ggatcactga
     1741 ccgacacggg gctctggatc accaatgcca caccgcgcgt cctggatgac attcgtgata
     1801 tttgccggca gctcttttca gttgaaccaa agacctgcac caaagccgga acaccagcgg
     1861 tttcgctttc aatttcttcg gtcaagctgg tgcaatggct tgaaaccgtg tgtggcatca
     1921 ctcgcggggc agccagcaag cggacgccgc agattatttc aagtgcgccg aaagagctga
     1981 tgctggcgtt tttcgaaggt cttttctggg gtgacggcac catcagcgcc cgtggccgac
     2041 tgggcagcaa ccggttcaag ctggcaactg cctcgcgtgt actggcgacc caactgcaag
     2101 tcgccctgtt gaatatgggt attgtggcag cggtccatga agagcgtatc aaaggcaagt
     2161 tcacggctta ctccgtgatt gtcaccggag acgccgtggt tgacctgatc gagctgattc
     2221 catcgctccg agacaagtgc accgatgacc cgtcacggct ggcgctcaag cggggacgaa
     2281 ccaattttaa cgtcattccc gaaatgcact ccgccgtgca cgccctgatg caggaagccc
     2341 gccgccaggg gcaatcactt tcaaccttcc gacgttatgc cacgacagcc gcctggggcc
     2401 ggaacctgac gccccaatcg ctggcccgtc tgattgaaga atctgaatgg ttgctgggcg
     2461 acgcttcccc agccctggag cttttgcgcg agcggcggga tgaaaacctg ctctggctcg
     2521 aagtgcaaag cattaccgaa ggtcacgccc acgtctatga cctgaccgtt cccggaacgc
     2581 attccttctg tgccaacggc tttatcaatc acaacagcca ggacatttct gaattgaccg
     2641 gctccattga tctttcctcc atcggcgaag tcggggtgga aagtgatccc cgcgcctacc
     2701 gatttgatgg agaacttaac attgcgaatc gggggattat ggagttcatc gaaattttga
     2761 agtgcattcg cggtaactcg ttgataagaa cgggattagg tgtgattcag tttgatgaat
     2821 tatggggaaa catgtggggt gaaaaacccc ctcatccgca agatgttcga tatcaaattg
     2881 gagtccaaac gagaaatgga atagaggaaa caactcatct gatttaccgg ggtgaagggg
     2941 aatctttgaa gatccgaact cgaaagggat atgttcttga aggtgcgcct gatcatcgga
     3001 ttttggtgct ttccatccgt ggccagttcg aatggcgggc attgaaagat ttagagccag
     3061 aggatcgagt gtgtattgat cgaaaaggat attggactga acagcttcc
//
LOCUS       DAOQDO010000520         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24586_length_3108_cov_3.754668, whole genome shotgun sequence.
ACCESSION   DAOQDO010000520 DAOQDO010000000
VERSION     DAOQDO010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24586_length_3108_cov_3.754668"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..732
                     /locus_tag="PKZ53_09430"
     CDS             16..732
                     /locus_tag="PKZ53_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ40699.1"
                     /translation="MLPDDSPLLESKEPQMESSLHYLQALILYVSLEWLWRERTDFFI
                     GANLSIYFKTEQLKHKTMVGPDLFVVTGTDRRPRQSWAVWKEGKFPDLIVELLSDSTE
                     KNDRTTKKEIYQNQFHTPEYFWFSPITLEFEGFQLIGARYQSIPANEHGHRWSEVLGL
                     FLGIENRHLRFFSADGTLVPTPEEAAQQAIQRAEAETQRAESEAQRAIVEQNRADAEA
                     RRVQKLMEQLRALGVEPEVE"
     gene            complement(905..1681)
                     /locus_tag="PKZ53_09435"
     CDS             complement(905..1681)
                     /locus_tag="PKZ53_09435"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNJ40700.1"
                     /translation="MLLVIDVGNTNTVLGVYQGEKLTNHWRLTTERERTVDEYGILCR
                     NLFEFAHLEPDKVDGIIIASVVPPLNFTLFRMAMVYFRQEPLFVRPTENVGMPIRYNS
                     PTDVGADRIVNAVAAFQRYGGPCVVVDFGTATTFDAISAEGDYLGGIITPGVNISSEA
                     LFQRAARLPRVDIRHPEKVIGDSTVTSIQSGLYFGYIGLVEGILKRMEEELGPLKAVI
                     ATGGLARLIGKGTSVITEIDDNLTLEGLRLIYLKNKSDKI"
     gene            complement(1800..2582)
                     /locus_tag="PKZ53_09440"
     CDS             complement(1800..2582)
                     /locus_tag="PKZ53_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNJ40701.1"
                     /translation="MKILMIGDVVGNAGVRLLTDRLRDLRKKHKIDFVVANAENIANG
                     VGILPHLAEELLAAGVDVMTSGNHAFDRKEATEYFKSEPRMLRPANYPEGVPGAGMWL
                     GEHNSGVSIAVINLMGRANMPRTDCQFRKADNLMKELSNRADIILIDHHAEATAEKIA
                     LARYLDGQVSAVVGTHTHVATADEQVLPAGTAYITDMGMTGSHSGVIGMSLDSVLERF
                     LSGLPVRLGSAEGDVRMNGVLIDVDTSTGHSTAISRLCVRHF"
     gene            complement(2698..2885)
                     /gene="ssrS"
                     /locus_tag="PKZ53_09445"
     ncRNA           complement(2698..2885)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKZ53_09445"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          838 a          784 c          752 g          734 t
ORIGIN      
        1 caggatgagc ttgctctgct cccagatgat tcaccactcc ttgaaagcaa agaaccccag
       61 atggaatcgt cacttcatta ccttcaagcc ttaatcttgt atgttagtct ggagtggctc
      121 tggcgtgagc gaacagattt ctttattggg gccaacctca gcatttactt caaaaccgaa
      181 caactgaagc acaagacgat ggttgggcca gacctgtttg tagtcaccgg cactgaccgt
      241 cgtccgcgac agtcctgggc ggtttggaag gaaggaaaat tcccggactt gattgttgag
      301 cttctgtcag actcaaccga aaagaatgat cgaaccacca aaaaagaaat ttaccagaat
      361 cagtttcata caccggagta cttctggttt tcaccgataa cccttgaatt tgaaggattt
      421 cagcttatcg gagcccgata tcaatcaatc cctgccaacg aacatggtca ccgctggagc
      481 gaggtattag ggttgttctt aggtattgaa aaccgacacc tccgattttt ttcggcggac
      541 ggcacgctcg tgcctactcc agaagaagca gctcagcaag caattcaacg cgctgaagct
      601 gaaacccaac gcgctgaatc tgaagcccaa cgcgcgatag ttgaacaaaa ccgggctgat
      661 gctgaagcac gtcgggttca aaaactgatg gagcaattgc gagcattagg ggttgagcct
      721 gaggttgaat agaggcaaat taagtacctt accgaagctt cccagacgtt tttaaccacg
      781 aaatacacga aaagaaccaa ccacttccaa aattcaatca atcaggaaac ttttgaaaag
      841 cactgactga acccaaattt ttcgctgtgc ccactgtggt gaggtttgtt ttgacttgct
      901 ctgctcaaat cttgtctgac ttattcttca gataaatcag ccgaagtcct tccaacgtca
      961 gattgtcgtc aatctcagtg atgacagacg ttccttttcc gatcaatcgc gccagcccgc
     1021 ccgtggcaat cacggcttta agcggtccaa gttcctcttc catccgcttt aaaatgcctt
     1081 ccaccaatcc aatatagcca aaatacaatc ctgactgaat gctggtcacg gtcgaatcgc
     1141 caatcacttt ctctgggtgg cggatgtcta cgcgaggaag ccgcgccgct cgttggaaca
     1201 gcgcttcaga ggaaatattg acacctgggg tgatgattcc gccaaggtag tcgccttcag
     1261 ccgaaatcgc gtcaaaggtg gttgccgttc caaagtccac cacaacacag gggccaccat
     1321 atcgctgaaa agccgccact gcgttcacaa ttcggtcagc cccaacgtcg gttggcgagt
     1381 tataccgaat tggcatgcct acgttttccg taggccgcac aaacaacggc tcctggcgaa
     1441 aatagaccat tgccatccga aacagtgtga agttgagtgg cggcacaacc gaagcaatga
     1501 tgataccgtc taccttgtcc ggttcaagat gagcaaactc aaacaggttg cggcacaaaa
     1561 tgccatattc atccaccgtc cgctcgcgtt cggttgtcaa ccgccagtgg ttggtcagtt
     1621 tttcgccctg atagacaccg aggacggtat tggtatttcc aacgtcaatg acaagaagca
     1681 taaaggcagg gttcagggtt ttcggaaggg atgagggatg agggatgagg gatgaaaaaa
     1741 gtcactttgt catcctgtca ccctgtcatc ttgtcagggt tcagggtttg gccctggtac
     1801 taaaagtgcc gcacgcacaa acggctaatc gccgtcgaat gacctgtcga ggtatcaaca
     1861 tcaatcaaca cgccattcat tcggacatcc ccttcggcgc ttccgagccg gacgggaaga
     1921 ccgctcagga aacgctccag cacgctatca agcgacattc caatcacacc tgagtgcgag
     1981 cctgtcattc ccatatcagt aatataggct gttccagcag gtaaaacctg ttcgtcagcc
     2041 gtggccacat gggtgtgtgt tcccaccacc gctgagacct ggccatcaag atatcgcgcg
     2101 agtgcaattt tttcagccgt tgcttcggcg tggtgatcaa tgagaatgat atctgcccgg
     2161 tttgaaagct ctttcatcag attgtctgct tttcggaact gacagtctgt gcggggcata
     2221 ttggcacgtc ccatgaggtt aatcaccgca attgaaacgc ccgaattgtg ttcgccaagc
     2281 cacatcccgg cccctggcac cccttccgga taattggctg ggcgcagcat gcgaggctca
     2341 gatttaaagt actcggtggc ttccttgcga tcaaaagcgt ggtttccaga agtcatcaca
     2401 tccacgccag ccgccagtaa ctcctcagcc agatggggga gaattcctac tccgttggca
     2461 atgttttcag cattggccac gacaaaatca attttgtgct ttttgcgcaa atcacggagg
     2521 cgatctgtca gcagacgaac tcccgcattg ccgacaacat caccaatcat caaaattttc
     2581 atagcagtgg aacgaatcag gttggaaggg gtgaggcgat tgaaacaggc aagggatttg
     2641 tcggtttagt tcgtgatgta cgacttcgcc actcacaaag aaatactttt ggagggaggt
     2701 ctctccgcgc agccgttgta gcagtctgtt tgaacctgtt tttcaacagg tgggtaacct
     2761 ggcttctgtc gtggcatcca cgtgcgctga cgagtattgc actgacagaa tatggtgcat
     2821 cggtttcccg ttcttacaat atcggcccaa acgagcaatg cccttcacta actttgcaga
     2881 gagacacgac ccagacggac aacttgtaat ctgcctcaaa gataattgct taaagcagtt
     2941 acagtgtttt gaatgatcat ttgcagaaac caaaactcta ctgcgcggaa aaggaaaaag
     3001 caaccaggaa tacccagttg ccagggagtg aagaatgaag aatgaagaat gaggaatgag
     3061 gaatgaagaa tgaagaatga agaatgaaga atgaagaatg aagaatga
//
LOCUS       DAOQDO010000521         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24595_length_3108_cov_2.288569, whole genome shotgun sequence.
ACCESSION   DAOQDO010000521 DAOQDO010000000
VERSION     DAOQDO010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24595_length_3108_cov_2.288569"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKZ53_09450"
     CDS             complement(<1..610)
                     /locus_tag="PKZ53_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ40702.1"
                     /translation="MRKEVHRWFSPHLGQEMPLAVYGHYGKPLLFFPTAAADFEEYER
                     FLVIDVLEPFVDAGIVKIFSIDSINRQSWMNDKMHPAQRAARQTAYDKYVTYEVAPFI
                     THHCNNPNIGIATTGASFGAYHCANVALKHPDVFDCMIAMSGSYDIRGYADGFHNEDV
                     YFNNPIEYIRNLNDDYYLPLLQHRSQLYILTGQGAYEAPDRSR"
     gene            1091..2023
                     /locus_tag="PKZ53_09455"
     CDS             1091..2023
                     /locus_tag="PKZ53_09455"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNJ40703.1"
                     /translation="MTELSPTYVIGAGSIGLFVQYLLQPIVSSVLVARPASCDRLNQS
                     PLRLTGAVESSLQMVCQPWETLTRQTLPTTLVIATKAHEVPEVLQMARVLFPPGSTLI
                     LCQNGIGIFESARHQLPDWTILRLHCWMGIQRRAPDHIHVAGIYKFDLAGAPEAEAHL
                     TNWHTILRQTPVPITIGRDPHSAEWQKALWNITVNGLCSIVNAPNGAILDFPELRTVA
                     ESIIFEAVAVAQAAGIALTQADVTTVFDSLEKTRTNINATLQDLRAGRHPELEFFNQA
                     VVQTAALTGCPAPLNTLVVQLVNYLEKTRQRKEV"
     gene            2030..2599
                     /locus_tag="PKZ53_09460"
     CDS             2030..2599
                     /locus_tag="PKZ53_09460"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019497484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNJ40704.1"
                     /translation="MEVTLEFLEIPILEATRENTADYGLFVGTDVPNAGLTIPFYKGS
                     VEEGHNIPFECNGNAVVRSARIHKRSGEVTWLERHLNMTQLFIGLGDQPFAMVLSKPN
                     HADEEEEPNLAEVRCFIFPPGHGIMLHKGTWHDFPLCVNEPVTCLTMNSPEVVEALAS
                     MKEPAEMNHGDVYKINIRDRMNTVLHVSL"
     gene            2635..>3108
                     /locus_tag="PKZ53_09465"
     CDS             2635..>3108
                     /locus_tag="PKZ53_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HNJ40705.1"
                     /translation="MYVQAFNLSTSGPADVSQLETLIQQGTIHPADVVCIIGKTEGNG
                     GRNDFTRDLAMYAFEDLFARHLGLSRAEVTQRIIFSLSGGCEGVVTPHIVVFTRAGTP
                     QSERRSAKRLAIGTGFTREFRPEEIGRIEQVVETSRVIHSIMEGMHLDSPKDVHFI"
BASE COUNT          793 a          824 c          749 g          742 t
ORIGIN      
        1 ggcgcgaccg atccggggct tcatacgccc cctggccagt cagaatatac aactgtgacc
       61 ggtgctggag cagcggcaaa taataatcat cattcaagtt acggatgtat tcgattggat
      121 tgttgaaata cacgtcttcg ttatgaaatc cgtcggcata gcctcgaata tcataggaac
      181 cagacatggc aatcatacag tcaaatacat ctgggtgttt taaggcaaca ttggcacagt
      241 gataggcgcc aaagctggcg ccagtcgtcg caatgccaat atttggattg ttgcagtgat
      301 gggtgataaa tggcgccact tcatacgtga catatttatc ataagccgtt tgtcgggcgg
      361 cgcgctgggc tgggtgcatt ttgtcattca tccagctttg gcggttgatg ctgtcaatcg
      421 aaaaaatctt gacaatgcca gcgtcaacaa atggctcaag cacatcaatc accagaaatc
      481 gctcatattc ttcaaaatca gccgcagccg ttgggaaaaa taaaagcggt ttgccatagt
      541 gaccatacac agcaagtggc atttcctggc ccaaatgggg actaaaccag cgatgaactt
      601 cttttcgcac gaattcctcc tcctcgatag caggtcaaag ttcccaaaat tgatggtaca
      661 ccaaagacat gaaatgttgg ggagtcttga ccgcaaccaa aactgaattg ttgggatatg
      721 gaagtctggc tcggaaagga tatgatgagc cgactgaccg gtagatgtgg gccaccatat
      781 cattgccaca cagcttcgct caagtctttg tttcaggaat ctccgggttc acgccaccca
      841 cttctgtcaa aattgaagaa acagagacaa ccacctggcg agaaacaatg caggttcatc
      901 cgtcctgagt atggctcaac ctgaggaggt caaccagcct gtgactgacc aacccctgta
      961 ttcgcaacac tcaacaaaca cttggttcgc tcaaggtttg tgctaaactg ccgccattct
     1021 tccaggctca ctcacaattc cgagcgctta tctcactcat catctcttct tctgggcggg
     1081 ggaaattctt ttgactgaac tcagtccaac ctatgtgatc ggtgctggca gcattggttt
     1141 atttgtccag tatctgctcc aacccatcgt ttcaagcgtc ctggtggcgc ggccagcgtc
     1201 gtgtgacagg ttgaatcaat cgccgttacg cctgactggt gcggttgaat cctcgcttca
     1261 gatggtgtgt cagccctggg agacattgac ccgacagact ttgcccacaa ccctggtcat
     1321 tgccaccaaa gcgcatgaag tgcccgaagt gctccagatg gcacgagttc ttttcccacc
     1381 tggaagcacc cttatcttgt gccaaaatgg aatcgggatc tttgaaagcg ccagacatca
     1441 gctcccagac tggaccattc tgcggcttca ttgctggatg gggattcagc ggcgggcacc
     1501 tgatcatatt cacgtcgctg gcatctataa atttgatctg gctggtgccc ccgaggctga
     1561 agcacacctg accaactggc acaccatcct tagacaaaca ccagttccaa tcaccattgg
     1621 ccgggatccg cactctgcgg aatggcaaaa agcgctgtgg aatatcactg tcaatggatt
     1681 atgttcgatt gtgaacgccc ccaacggcgc cattctggat tttcccgaac tgcgaaccgt
     1741 ggccgaatca atcattttcg aagccgtagc tgtggctcag gcggctggta tcgctctgac
     1801 acaggcggat gtcacgacgg tttttgattc actggaaaaa acacgcacca atatcaatgc
     1861 cacattgcaa gacctccggg ctggacgcca cccggaactt gagttcttta accaggccgt
     1921 tgtccagaca gcggccctca ctggatgccc ggcaccattg aacacgctgg tggttcaact
     1981 ggtgaattat cttgaaaaaa ccaggcagcg aaaggaagtc tgaaccccca tggaagttac
     2041 ccttgaattt cttgagattc caattcttga agccactcgc gaaaacaccg ccgattacgg
     2101 gctgtttgtc ggcactgacg tcccgaatgc cgggttgacc attccatttt ataaaggatc
     2161 tgtcgaagaa ggacataaca ttcccttcga atgcaacggc aatgcggttg tcagatcagc
     2221 ccgcattcat aaacggagcg gcgaggtaac ctggcttgaa cgccatctca atatgaccca
     2281 attatttatc ggattgggtg atcaaccgtt tgccatggta ctgagcaaac ccaatcacgc
     2341 tgacgaggaa gaggaaccaa acctggccga ggttcgatgt tttatttttc cgccaggcca
     2401 cggaatcatg ctccacaaag gcacctggca cgactttccg ctgtgcgtca atgaaccagt
     2461 gacctgtctg acgatgaatt cgcccgaagt ggtcgaagcc ctcgccagca tgaaagaacc
     2521 agccgaaatg aaccacggcg atgtgtataa aatcaatatt cgtgaccgga tgaacaccgt
     2581 tctccatgtc tcactctaag ggaaaagcca cccacacctg gccgattttt gtctatgtac
     2641 gttcaagcat ttaatctttc aaccagtggc ccggctgatg tcagtcaact tgaaaccctg
     2701 atccagcaag gaacgattca tcccgctgat gtcgtctgta tcattggcaa aaccgaaggc
     2761 aacggaggtc gcaatgattt tacccgcgat ctggccatgt atgccttcga ggacctgttt
     2821 gcccgccacc ttggcctgtc acgagccgaa gtgacgcaac ggatcatttt ttccctgtcg
     2881 ggcgggtgtg aaggggtggt aacaccacat attgtggttt ttacccgggc tggtacaccc
     2941 caatctgaac gacgatcagc aaagcgcctg gcgattggca ctggctttac ccgtgaattc
     3001 cgccccgaag aaattgggag aatcgagcaa gtcgttgaaa catcacgggt gatccattca
     3061 attatggaag gaatgcacct tgactcccca aaagatgttc atttcatc
//
LOCUS       DAOQDO010000522         3104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24655_length_3104_cov_3.928501, whole genome shotgun sequence.
ACCESSION   DAOQDO010000522 DAOQDO010000000
VERSION     DAOQDO010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24655_length_3104_cov_3.928501"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1262
                     /locus_tag="PKZ53_09470"
     CDS             111..1262
                     /locus_tag="PKZ53_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40706.1"
                     /translation="MKEHQIQKSGTRNPEPGTLFLLLLLVLTGTSTALAKEQLPFPLT
                     ARTIEEFVPTDWTILEKAEGDLNGDNQPDVVLALAPDSTEDEEADRSRWLLIAFREGK
                     IWRRSIVSDQAILCRNCGGVFGDPFAGVRIERGSVVVEHYGGSAWRWAITDRFRWQQG
                     QWVRIGNTTASFHTFEPDDVEQLDVNLLTGFAEEQTNREKGSKTLSFFQIRAGKSTSS
                     PNLDGIISASEWPGTSVILKSQADIVSGRSGWRGKDDLSARLGALVVGKILYLRAEIT
                     DDQVTAKESVHLISSNREVIKPTSSQTKLVPGGYVFEAAYPLSLLTSDSNPEITVPLE
                     LRVSIEVVDSDPDEKTATILSTSRGGQTYPAMIYVTEKPGPEILEELAK"
     gene            1293..1985
                     /locus_tag="PKZ53_09475"
     CDS             1293..1985
                     /locus_tag="PKZ53_09475"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNJ40707.1"
                     /translation="MTKHIGIVACSTEGAALCYRTISLVGAELMGRHAHPQVSLHSHP
                     LSEYMKCIDADSWQGVANLMLSSTHKVAQTGADFAICPDNTIHQALDFFKETSPIPWL
                     HIAEEVGAEANRRGFKRLAILGTKYLMEGPVYPSRLEPLGLDYRIPNLEERIRINDHI
                     FDELVYGRLTDEARAFFVKVITRLQTEEGCDAAVLGCTEIPLLISDADSPLPTLDSTR
                     LLARAAVQYALR"
     gene            complement(2031..2909)
                     /locus_tag="PKZ53_09480"
     CDS             complement(2031..2909)
                     /locus_tag="PKZ53_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNJ40708.1"
                     /translation="MACLSLLVTVGLTLGITNEVHSFYLLIKEQSGQPGGFFETLSHT
                     FERARQWLGQYVDMAQFDLRATVLGQAEQISKLVLAQTANVLGNIVAVVVEAVITFLT
                     LFFFLRDGRAIWRRLSVWFPLRRDQVEKLSTGVSSMITASVYGMAAVGAAQGTLTCIA
                     FVALGLPSPILWGVVAGVFSLIPLVGSSVIWVPTAIILFLTGHWIKAVIMLAWGALVI
                     GMADNVIRPIILSGSTKTSYLLTFFALLGGVRAFGLIGLFLGPLVLALTQTLFQLLQE
                     EGNWWAPDTPDDNVTE"
BASE COUNT          812 a          855 c          762 g          675 t
ORIGIN      
        1 ttccgggttc cgggttccgg gttccgggtt ccgggttccg ggttccgggt tccgttcgaa
       61 agatccaatg attccagtca tttcaagcac cacgaagagg gagagaccaa atgaaagaac
      121 atcaaattca aaaatccgga acccggaacc cggaacccgg aaccctattc ctgctcctgc
      181 tcctcgttct caccgggaca tccacggccc tggcaaaaga acaacttccc ttcccactta
      241 cagcgcgcac gattgaagaa tttgtcccga cagattggac aatccttgaa aaagcagaag
      301 gcgacctcaa cggcgacaac cagccagatg tggttctggc gctggcacct gactcaaccg
      361 aagacgaaga agcggatcgc agccgctggc tgctgattgc ctttcgtgaa ggcaaaatct
      421 ggcggcggtc aattgtttcc gatcaggcaa ttttgtgccg taactgtggt ggcgtatttg
      481 gtgacccgtt tgccggcgtg cggatagaac gtggatccgt ggtggtcgaa cactatggcg
      541 gctccgcctg gagatgggcg atcactgacc ggtttcgctg gcagcaaggt cagtgggtac
      601 ggattggcaa taccaccgcc tcatttcata ccttcgaacc cgatgacgta gagcaacttg
      661 acgtcaacct cctgacgggc tttgccgaag aacaaaccaa tcgcgaaaaa ggctcaaaga
      721 cgctttcttt tttccaaatt cgagctggga aaagtacgtc gtcaccaaat ctggatggca
      781 tcatttctgc cagcgaatgg ccaggcactt cggttatact taaatcacag gccgatattg
      841 tcagtggacg gtcaggctgg cgtggaaaag acgatctttc agcacgactc ggagcgctgg
      901 tcgttggcaa aatcctctat ctgcgggctg aaatcactga cgatcaggtg accgccaaag
      961 agagcgtgca ccttatttca agcaaccgcg aagtcatcaa accaaccagc agccagacca
     1021 aactggttcc aggcggatat gtatttgaag ccgcttatcc actcagtttg ttgacatctg
     1081 attcaaaccc ggaaattacc gttccacttg aactccgtgt ttcaattgaa gttgttgatt
     1141 ctgacccgga cgaaaaaacg gctacaatac tttcaaccag ccgtggtggg caaacctacc
     1201 cggcgatgat ctatgtgacg gaaaaaccag gcccagaaat tcttgaagag ttagcgaagt
     1261 aataatctgc gcgatttcac tcctaaaacc ttatgactaa acacattgga attgttgcct
     1321 gcagcaccga aggagctgcg ctctgctatc gaaccattag ccttgtgggt gctgagttga
     1381 tgggtcgcca cgctcatcca caagtttcgc tccattctca tccgctcagt gaatatatga
     1441 agtgcattga cgctgatagc tggcagggcg tcgccaactt gatgctctct tcgacccaca
     1501 aagtcgccca aaccggtgca gactttgcca tttgtccaga taacaccatc catcaggcac
     1561 ttgatttctt caaagaaacc tcaccgattc cctggctgca cattgccgaa gaggtcggag
     1621 ccgaagccaa ccgacgcgga ttcaagcggt tggccattct cggcacgaaa tatctgatgg
     1681 aaggcccggt gtatccgagc cgattagaac cactgggcct cgactatcgc attcccaacc
     1741 tggaagaacg catccgcatc aacgatcaca tttttgatga actggtgtat ggacgtctga
     1801 cggatgaagc ccgcgccttt tttgtgaagg taattacacg gcttcaaacc gaggaaggct
     1861 gtgatgcggc agtattggga tgtactgaaa ttccgctttt gatcagtgat gctgattcgc
     1921 cgctgccaac gcttgattca acccggttgc tggccagggc ggcagtccaa tatgcacttc
     1981 ggtgactcat aacaaggtaa ctgggtgaca attgccattt ggtcacattg tcactctgtt
     2041 acattgtcat ccggggtatc gggtgcccac cagtttcctt cttcctgtaa aagttgaaac
     2101 agcgtttggg tcagcgccag aaccagcggc ccaagaaata atccaatcaa tccaaaggcc
     2161 ctgactccac ccagaagtgc aaaaaatgtc agcagatacg aagttttcgt cgagccactg
     2221 aggataattg ggcgaatcac attgtcggcc atgccaatca ccagggcgcc ccaggcaagc
     2281 ataataacgg ctttgatcca gtgcccggtg agaaacaaaa taattgccgt tggcacccag
     2341 atcacactcg atccaaccag gggaatgagc gaaaacactc cggccaccac cccccacaaa
     2401 atcggagaag gtaacccaag tgcgacgaag gcaatacagg tcagtgttcc ctgtgccgcc
     2461 cccacggcgg ccatgccata gacgctggca gtgatcatgc tgctgacacc ggttgagagc
     2521 ttttccacct gatctcgccg aagcggaaac cagacagaaa gtcgccgcca tatcgcccgc
     2581 ccatcacgca aaaagaaaaa gagcgtcaga aacgtgatga cggcttcgac caccaccgcc
     2641 acaatattcc cgagcacatt ggctgtttgt gccagcacca acttactgat ttgttcggcc
     2701 tgcccgagaa ccgtcgcccg cagatcaaac tgagccatat caacatattg acccagccac
     2761 tgtctggctc gttcaaaggt gtgcgagagc gtttcaaaga agcctcccgg ctggccactt
     2821 tgttctttga tcaataaata aaaggaatga acttcgtttg tgatgcccag ggtcagtccc
     2881 actgtcacaa gcaggctcag acaggcaatt gccagggttg aaaccagtgc cgcctgattt
     2941 ggattgcgaa tccggctgac gacccatcgg cgatgtgccg gttacacaac caccgccaga
     3001 ataatggcca gaaaaatcgg ctccagaaac ggcccggcaa tcacataaca aaacccaagg
     3061 gccacaatgg ccaaactcat taaaaatgcg actctgactt tttt
//
LOCUS       DAOQDO010000523         3104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24659_length_3104_cov_3.365038, whole genome shotgun sequence.
ACCESSION   DAOQDO010000523 DAOQDO010000000
VERSION     DAOQDO010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24659_length_3104_cov_3.365038"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PKZ53_09485"
     CDS             complement(<1..1297)
                     /locus_tag="PKZ53_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ40709.1"
                     /translation="MKIFFQKVSLLQQSLYTQIFSRSSFSPRTSRQILGITLVSIGLL
                     GSLGLWGGLISQAQTAPQITSLSPSQTSVGQVPKKMTIIGSGFAAGAQVEVSSSASTV
                     TIAAKVSGSTQLVIKGKKLPAAFFAQDGQLRVVVISNGQRSNAVTFQVGNGGSAGGGV
                     QFQSVDPVLQEGGTLQVRAQVVDSQGNPLPGTVTYESGSPEIATVEASTGLVRGVTRG
                     FATITARSGNLSAGLTLTVTRIENSSDTNNGDVVLIRDQAGRLYSSNPTRHTINRRDG
                     FGQASRLFAGRDQVPGNVDGTLTQSLFNSPIGLSIDDRSGRIYVADTANHVIRAMLPS
                     GNVITIAGIKNQAGSTDGAVTSATFRNPRAVEVDLGGNLYVADTENHTIRYIDFSTNT
                     VSTIAGVAGQAGLQDGVGSGARFSRPTGLRLSPDKRILNV"
     gene            1478..2296
                     /gene="proC"
                     /locus_tag="PKZ53_09490"
     CDS             1478..2296
                     /gene="proC"
                     /locus_tag="PKZ53_09490"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNJ40710.1"
                     /translation="MLRGKKIAIIGAGKLGEALVTGLLHRADVSREDITGTVGHEASL
                     TRVSNRLGIGTTLDNRLAVEGRDVILLAVKPQNMDRVLAEIRPVVNRTQLFISVAASV
                     TTSFIEERLPAETPVIRVMPNTPALLNEGMSVLCQGKHVTPDQLKIAETIFSAVGRTA
                     FLDEKLMDAVTGLSGSGPAYIYVVIESLAEAGVKLGIPRDISTLLVAQTMLGASRMAL
                     ETGQHPALLKDSVTTPAGCTIDGLLELEEGKLRVTLIKAVVKAAERARELVRTE"
     gene            2364..>3104
                     /locus_tag="PKZ53_09495"
     CDS             2364..>3104
                     /locus_tag="PKZ53_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40711.1"
                     /translation="MKRVQFRLIAASFCIAGLMGCTSPAVPSPAPPPVPAPPPVPSTS
                     RVDLPTQFDKVFTGTIGEKEATLYLNRSGEDLGGAYVFEEPRPLLHGRKNAFLLEGKL
                     QADGQFSLGIQQLEQPRGTLTGKLKVETQPQGKQLVLEGDWTSPGQSAPTKIKFAEEL
                     QVLGNGVELAEQVRNDENQPANYVFQARYPQLSGATDEISRQFNQKVELLVDEAYHSF
                     KAEVAKNYSHPPPSPTDSTVTSQLNSMGI"
BASE COUNT          758 a          813 c          824 g          709 t
ORIGIN      
        1 tcacatttaa aatgcgtttg tccgggctca gtcgcagccc cgttggacgt gaaaaccggg
       61 cgccgcttcc aaccccatcc tgcagaccag cctggccggc cactccggca atggtggaaa
      121 cggtgttggt cgaaaaatca atatagcgaa tggtgtgatt ttcggtatcc gccacgtaaa
      181 gattgccgcc aagatcaacc tcaaccgccc gagggttgcg gaacgtggct gatgtcacgg
      241 cgccatcagt cgagccggcc tgatttttga ttcccgcaat tgtgatgaca tttcctgagg
      301 gcagcatggc ccgaatgacg tggttggcgg tgtctgcgac gtaaatgcga ccactccggt
      361 catcaatact taacccaatg gggctgttaa acagggactg ggtgagtgtt ccgtcaacat
      421 tccctggtac ctggtctcgc ccggcaaaca agcgtgatgc ctgtccaaac ccatctcgtc
      481 ggttgatggt gtgccgggtt ggatttgagc tatagaggcg accagcctgg tcgcgaatca
      541 acaccacgtc gccgttattg gtgtcacttg agttttcaat gcgggtcacg gtcagggtca
      601 agccggctga tagattgcct gaccgggctg taatcgtggc aaatccacgc gtcacacccc
      661 ggaccagtcc agtactggcc tcaaccgttg caatttcagg gctcccagac tcataggtaa
      721 ctgtgcctgg aaggggattt ccctgtgaat caaccacctg agcccgtacc tgcaaggtgc
      781 cgccttcctg caaaactggg tcaaccgatt gaaattgaac cccgccaccg gctgaaccac
      841 cgttcccaac ttgaaaggtg acagcgtttg agcgctggcc gttgctgatc accacaactc
      901 ggagctggcc atcctgggca aaaaaagcgg ctgggagttt tttgcctttg atcaccagtt
      961 gggtgctgcc agagactttg gctgcaatcg tgaccgtcga agcagagctt gaaacctcaa
     1021 cctgagcccc ggcagcaaat ccggaaccaa tgatggtcat tttctttgga acctgcccaa
     1081 cgctggtttg cgaaggtgaa agactggtga tttggggggc cgtctgggcc tgggagatca
     1141 atcctcccca caagcccaga ctccctaaca gtccaatcga aaccagggta attcccaaaa
     1201 tttgacggct ggtgcgtggc gagaaagagg agcgtgaaaa aatctgtgtg taaagtgact
     1261 gctgcaataa cgatactttt tgaaaaaaaa tcttcataga aacctcaatc gtgtttctca
     1321 aatattggtc gaaaaccaaa gaataccatc ttcatgcgtg tcttggggta gaaagaaacc
     1381 tgactgtacg acaaatttac cggttctggc ttgttgtttt gctgaaaaat cagatagaaa
     1441 tgaaagtctt ttacatttcg cagggaaagg tcattcaatg ttacgtggta agaaaattgc
     1501 aatcattggt gctgggaagc tcggcgaagc cctggtcacc ggcctgttac accgggctga
     1561 tgtcagccgg gaagacatca ctggaacggt tggtcatgaa gcctcgctga cccgggtctc
     1621 aaatcggttg ggcatcggca ccacgctgga caatcgcctt gccgttgaag gacgcgatgt
     1681 cattttgctg gcagtcaaac cccaaaacat ggaccgggta ctggctgaga tccgacccgt
     1741 agtcaatcga acgcaactct ttatcagcgt tgccgcctcg gtcacgactt cttttattga
     1801 agagcggctg ccggctgaaa ccccggtcat tcgagtgatg cccaacacgc ctgccttact
     1861 caatgaaggg atgtcagtcc tttgccaggg aaaacatgtc acgcctgatc aattaaaaat
     1921 tgctgagacc attttttctg ccgtcggtcg aaccgcgttt ctggatgaaa aactgatgga
     1981 tgcggtgact gggctttcag ggagtggacc agcttatatt tatgttgtca ttgaatcgct
     2041 ggctgaagct ggcgtgaaac ttggaattcc tcgtgatatc tcgacgttgc tggtggcaca
     2101 aacgatgctt ggcgcgtccc gcatggcttt ggaaaccggt cagcacccgg cattgctcaa
     2161 agacagcgtg accaccccgg ctggatgcac cattgatggg ctgcttgaac ttgaagaagg
     2221 aaaacttcgg gtaaccttaa ttaaggcggt ggtgaaagcc gccgagcggg cccgcgaact
     2281 ggtccgaacc gaataaatag cgtcgttttt caccatcttc tgatccatcc cgcctgagcg
     2341 gtttttaacc ttcgaaaaaa agaatgaaac gtgttcaatt cagattgatc gctgccagtt
     2401 tctgcattgc tggactgatg ggctgtacct cgcctgctgt accatcaccg gcgccccctc
     2461 cggtgccagc gcccccacct gttccttcga cttccagggt ggatcttcca actcagtttg
     2521 ataaggtctt tactggaacg attggagaaa aagaagccac attgtacctg aatcgatcag
     2581 gtgaagacct gggcggggcc tatgtctttg aagagccccg accattgctc cacggacgga
     2641 agaacgcgtt cctgctcgaa ggaaaacttc aggccgatgg tcagttttca cttggcattc
     2701 agcaactcga acagccacga ggcactctga ccgggaagtt gaaggtcgag acccagcccc
     2761 aggggaaaca gcttgtgctg gaaggcgact ggacatcacc cggccagtcc gcacccacaa
     2821 aaatcaagtt tgctgaagaa cttcaggtgc ttgggaatgg tgttgaactc gccgagcagg
     2881 ttcgaaacga tgaaaatcag ccggccaact atgttttcca ggcccggtac ccgcaactgt
     2941 caggggccac agatgagata agccggcagt ttaatcagaa agttgaattg ctggtggatg
     3001 aggcatatca ctcattcaaa gcggaagtgg ccaaaaacta cagccatcca ccaccgtcac
     3061 caactgactc cacggtgaca agccagctca attcaatggg aatt
//
LOCUS       DAOQDO010000524         3104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24670_length_3104_cov_1.884224, whole genome shotgun sequence.
ACCESSION   DAOQDO010000524 DAOQDO010000000
VERSION     DAOQDO010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24670_length_3104_cov_1.884224"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKZ53_09500"
     CDS             <1..798
                     /locus_tag="PKZ53_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ40712.1"
                     /translation="LAQCEISYVPSATFASRLLQRESGPIHQAESMLVMADPVFTLAD
                     SRLSQVARVNGTKSERVEIADSTHAFRGLRFSRLPASSAEAALIERLFKGKVVKKLGF
                     DASRSILSDVKANQYQMIHLATHSVIREKDPALSGLLFSLLDAQGNAQDGFLTLTDIA
                     NLPLKADLVVISACQTADGKLIEGEGPLSFSRAFLLAGARTVIGTQWAVDDKATSELM
                     KLFYEQLLTRHLPPVKALRQAQLQMLKNTTYQSPEHWAAFECYGDWR"
     gene            complement(780..1688)
                     /locus_tag="PKZ53_09505"
     CDS             complement(780..1688)
                     /locus_tag="PKZ53_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40713.1"
                     /translation="MSIPSPRSVPLPADDDSMAPDPVVASFLRSEPFAEPPAALRDRV
                     LQAFEAHFEQYPITSDLETLEDDSEAELWKSAKPWMAPQPSMWLRAAPYAFGLGMGAL
                     ALAGSLLVWNARQPAQLVMVEFPHPALSLPDPMAVAPPTPVVKPAPLETNPKPASGKV
                     VKTEAHNYRSEVPRTLLATAAPADVKFFAGQLTLSVDGVEVTQTKSSTAGSAIRVSLG
                     GNGDYILMLSPKNDSFQPNGWVEGKQFWFEVNGQTVEIQCSEAIVSGSGQFQLWVSTI
                     PAGSDHTTGFGVRLYSSFDAAQRQSP"
     gene            complement(1719..2270)
                     /locus_tag="PKZ53_09510"
     CDS             complement(1719..2270)
                     /locus_tag="PKZ53_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ40714.1"
                     /translation="MITRPTDHQLLVRASRGDKAAFAELYDRYHRSVWGFLLKMTHDD
                     TLTSDLTQEVFLEVLKCPDRFDPTRGASMETWLCAIARNLYLMHYRKTGRETSEDTSI
                     PESAPMGTRSSSRSAFETVAIARIDVRRALSNLSFEQREVLVLADMEDHQVKEISFII
                     GISVAAVKSRLLRARENVRKLLI"
     gene            2903..>3104
                     /locus_tag="PKZ53_09515"
     CDS             2903..>3104
                     /locus_tag="PKZ53_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40715.1"
                     /translation="MLTSIQRLSLHNSGADWSRDILSAVVQAHNSLWVISGKRNRLDC
                     FAPTGGVHFKAMKSFDLDHFLAD"
BASE COUNT          776 a          744 c          790 g          794 t
ORIGIN      
        1 ctggcccaat gcgaaatcag ttatgttccg tccgcgacct ttgccagccg gttgcttcaa
       61 cgcgaatcag gaccaattca ccaggcagag tcaatgctgg tgatggcaga tccggtgttt
      121 acgctggctg attccagatt gtcccaggtt gcccgcgtga acggaacgaa gtctgagcgg
      181 gtcgaaatcg ctgactccac gcacgcattt cggggacttc gcttttcacg tctcccggcc
      241 tcaagcgccg aagccgcttt aattgagcga ttatttaagg gaaaagttgt caagaaactg
      301 gggtttgatg ccagtcggag tattttgtct gacgtcaaag caaatcagta tcagatgatc
      361 caccttgcga cgcatagtgt gattcgggaa aaggacccgg ctctttccgg attgctgttt
      421 tcgttgctgg atgctcaggg caatgcccag gatgggtttt tgacgttgac tgatattgcc
      481 aatctaccat taaaagctga tttagttgtg attagcgcct gtcagaccgc cgatggaaaa
      541 ttgattgagg gcgaaggacc gctttccttt tcacgtgcct ttttactggc tggggcccgc
      601 acggtgattg gcacgcagtg ggcagtagat gacaaagcca cttcagaatt gatgaaactc
      661 ttttacgagc aattacttac ccggcatctt ccaccagtca aggctttgcg tcaggcccag
      721 ttgcaaatgc tcaaaaacac tacctatcag tcacccgaac actgggcagc ctttgaatgt
      781 tacggtgact ggcgttgagc cgcatcaaat gacgaataga gtcggacccc aaatccggtc
      841 gtgtgatcag aaccggctgg aatggttgaa acccagagtt gaaactgccc tgatccactc
      901 acaattgctt cagaacattg aatttccact gtttgtccgt tgacttcaaa ccaaaattgc
      961 tttccctcga cccatccatt gggttgaaac gaatcatttt tgggagacag cattaaaata
     1021 tagtcaccgt tcccgcccaa tgagactcga atggcacttc cagccgtgga tgatttggtt
     1081 tgggtgactt caaccccatc aaccgaaagg gtcagttgcc cggcaaaaaa tttcacgtct
     1141 gctggtgctg cggttgccag caaggtgcga gggacttctg atcggtaatt gtgagcttca
     1201 gttttgacca cctttcctga ggctggtttc ggatttgttt cgagtggggc tggttttaca
     1261 accggagttg gtggtgccac cgccattgga tcgggtaatg agagtgcggg gtgtggaaac
     1321 tcaaccatga ccagttgagc tggctggcgg gcattccata ccagaagtga tccggcaagt
     1381 gccagcgcgc ccatccccag gccaaacgca tagggtgccg cccgaagcca catcgagggt
     1441 tgcggcgcca tccagggttt ggcgcttttc cagagttcag cttcggaatc atcttccagg
     1501 gtttccaggt cagatgtgat cggatattgt tcaaaatgtg cttcaaaagc ctgcaataca
     1561 cggtctcgaa gggcggctgg cggttcggca aacggctctg acctcaaaaa tgaggcaaca
     1621 actggatccg gagccatgct gtcatcatca gcaggtaatg gaaccgaacg gggtgatgga
     1681 atagacataa aaacagcctc tcctgggcaa attcagcgtt atatcaagag ttttcgaaca
     1741 ttttcacgtg cgcgtaaaag tcttgatttt actgctgcca ctgaaatgcc aataataaaa
     1801 ctaatttctt tgacttgatg atcttccata tccgccagga caagcacctc tcgttgttca
     1861 aaggacaggt ttgaaagtgc acggcggaca tcaatgcggg caatcgccac ggtttcaaac
     1921 gcagaacgac tggaactgcg cgttcccatc ggagctgatt ccggtatgga tgtatcttca
     1981 gatgtttcac ggcctgtttt tcgataatgc ataagataca gattccgtgc aatcgcacac
     2041 aaccaggttt ccatacttgc cccacgggtg ggatcaaacc ggtctggaca cttgagtacc
     2101 tccaggaata cttcctgtgt caggtctgaa gtgagggtgt cgtcatgggt cattttcaag
     2161 agaaatcccc agactgaccg atgataacgg tcatataatt ctgcaaacgc ggctttgtca
     2221 cctcgtgagg ctctgactaa taactggtgg tcagttggcc gggtaatcat tcaatcaact
     2281 cctctgatta tccaaattat gactcagcgc gggagacaaa caggttaaga catgcaatga
     2341 agccaggcgg caagtcaagg caatcgaaat tcgggtgaga tgaacccagt ggaatagtaa
     2401 gaataattga tgcacttggt acgcagaagt gtatctcagt gaaaaatcca gggaaagtcc
     2461 aagagagtac agaattccgg attcttccga gaaattaccg gaaatttaaa aaaaattttc
     2521 aaaagctgaa tcatttttgc cttctcgtgc aaataaccag taaacactga ggtcttccat
     2581 ttcatagttc gcgcatcact ctccccgggt gaaagttgcg gtggccaaaa agtggaggaa
     2641 gatttcagtg agctttttgg gtccagaaaa cgacgtgttc ctgccagtct ggattgattg
     2701 ctgcgtcctc atccagtggg gatcattcca gaaaggtcct gtccagtccc gtgattgggg
     2761 tgcccctccc acaggtgcct tccaaaatcg gatgtgttta ccaggttaaa ctcctggcac
     2821 tgagttggct ttaaccccca acctcccggt gccagtgaat caaatagaaa ggattcttcg
     2881 gtgcacctta gggggtgcat tcatgctgac atccatccag cggctttcgc tccacaacag
     2941 tggtgccgac tggtccagag acatactttc ggctgtggtg caggctcaca acagtttgtg
     3001 ggtaatttct ggaaaaagga atcgtctgga ttgttttgct ccaaccggag gtgtgcattt
     3061 taaagccatg aaatcatttg acttagatca ttttcttgct gatt
//
LOCUS       DAOQDO010000525         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24702_length_3102_cov_2.695110, whole genome shotgun sequence.
ACCESSION   DAOQDO010000525 DAOQDO010000000
VERSION     DAOQDO010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24702_length_3102_cov_2.695110"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKZ53_09520"
     CDS             <1..299
                     /locus_tag="PKZ53_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40716.1"
                     /translation="VAFNILEPEGPDSALRWGRFDSIFEQKEYFSDYVMEPIARKMLE
                     LNPKLREEFEQRLKNDAQFAADPQARLYFFYQRSPYFEADKDVYPVVRVMHAFK"
     gene            362..592
                     /locus_tag="PKZ53_09525"
     CDS             362..592
                     /locus_tag="PKZ53_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNJ40717.1"
                     /translation="MAATKITINPNGSLRIEGDFEIFDAQGRQFGLAGRTVIGLCRCG
                     HSVNKPFCDGAHGRNGFESVCEARELPAPVKK"
     gene            935..1285
                     /locus_tag="PKZ53_09530"
     CDS             935..1285
                     /locus_tag="PKZ53_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40718.1"
                     /translation="MARIQFLRLLASTRQNRFEMVHLLQQAIQHSGGWIVDFRQFSNQ
                     AGVIFFETEGRNAKALELEFLKVPVCWTEESLTEMKNLSAHPVPDSEVRGSIHLTFIH
                     TEPDVRIAVPAVPG"
     gene            complement(1296..2273)
                     /locus_tag="PKZ53_09535"
     CDS             complement(1296..2273)
                     /locus_tag="PKZ53_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ40719.1"
                     /translation="MKNQLSHLFCSLLSILSILPQPILSQTPIKPAPEPNKIAITFDD
                     LPLNGRQTSVVLQHKMTRRLIHTICANQIAAVGFVNEAQLFQYGEIDRRVELLKLWVD
                     AGLELGNHTYSHPSLHETPVDVFTEDIIRGETVTKMLLKPKQLPLRYFRHPYLRVGQS
                     METRTQIESFLKHHGYTIAPVTIENSDWMFNALYTPAKLAGNKQDMKSVSEAYLTYTE
                     TMFEYYEKLSQDILGRQVRHILLLHANELNADYFDELVSLMKKRGYSFISLEEALADP
                     GYLQTDTYVGKGGISWLHRWAISKGLKPGLLGKPEPPADIQKSFDQLGY"
     gene            2707..>3102
                     /locus_tag="PKZ53_09540"
     CDS             2707..>3102
                     /locus_tag="PKZ53_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNJ40720.1"
                     /translation="MFRHWFFSFLFIISLGLLLAGHTSVQAQYRQLEEPPPMQGSPEI
                     LKKIGIDQKLNAQIPLTLPFKDETGRDISLQEYFGTKPVILAPVYYECPMLCTLTING
                     VVQSLDQLKTVAGKDFLVVAVSFNPLETPD"
BASE COUNT          809 a          693 c          747 g          853 t
ORIGIN      
        1 gggtggcgtt taacatcctg gaaccagagg gacctgattc cgcgctgcgg tggggccggt
       61 ttgactcgat ctttgagcaa aaagaatact tctcagacta tgtgatggag ccgattgccc
      121 gcaaaatgtt ggagttaaac cccaaactgc gcgaagagtt tgagcagcga ctcaagaatg
      181 atgcacaatt tgccgccgac ccccaggccc gtctgtactt tttttatcaa cgctcaccct
      241 actttgaagc cgacaaagat gtgtacccgg tcgtacgggt gatgcatgcc ttcaaataac
      301 cagtcgacag ggttgaatgg cagttcaacc tcaatttcag ttcatcagga gaactcacac
      361 tatggcagcc accaaaatta caattaaccc caatggatcg ctccggatcg aaggcgattt
      421 tgaaattttc gatgcccagg gacgtcaatt tgggctggcc ggtcgcaccg tcattggatt
      481 atgtcggtgt gggcactcag tcaataaacc cttttgtgat ggagcccatg gccgaaatgg
      541 ctttgaatcg gtttgcgaag ctcgtgaact tccggcccca gtcaagaaat aataccagct
      601 tgtagtcagt agatcggagt cagtagttca ctaactctat ttgattgaat tacctgacta
      661 aaactctgaa ccctgattgg gataacacct gatcccgcta cgaagaaaaa aaatgagaat
      721 tgagttgtgg agacgagaaa cacgccgcat tttgatgttt caactcgggc cacaacccaa
      781 ttctcatcat taacacttct caggttcagt ggctgatgaa atcaagggaa aaaaattaca
      841 cccggcagag aatttttttc ggaaaaaagt ccacctcccc gcgaaaacct cctatatctt
      901 tgatttacat agactttaat tagttcgaaa tcccatggcc cgcattcaat ttctccggtt
      961 attagcctca acccggcaaa atcgctttga aatggttcat ctcctgcaac aggcaatcca
     1021 gcacagcggc gggtggattg tggattttcg gcaattttcc aaccaggctg gcgtgatttt
     1081 ctttgagacc gaaggccgca atgcgaaggc actcgaactt gaattcctga aagttccggt
     1141 ttgctggacc gaggaaagtc tgactgagat gaagaacctg tcagcgcacc ctgttcccga
     1201 tagtgaggtc agaggctcaa tccatctcac attcatccat actgagccgg atgtgcggat
     1261 tgcagtaccg gcggtgcctg gttgaaccag ggcatttaat atccaagttg atcaaatgac
     1321 ttctggatat cggctggtgg ttccggcttt cccagcagac ccggtttcaa tccctttgaa
     1381 atggcccacc ggtgaagcca ggaaatgcct ccctttccga cataggtatc agtctgcaaa
     1441 tagcctgggt cagccagtgc ctcctcaagt gagataaaag aatatccgcg ctttttcatc
     1501 aagctgacca gttcatcaaa atagtcagca tttaactcgt ttgcgtgcaa caacaagatg
     1561 tgacgaacct gtcggcccag aatatcctgc gaaagctttt cataatattc aaacatggtt
     1621 tcggtatagg tcaggtaggc ctcactcact gacttcatgt cctgcttgtt tccggcaagt
     1681 ttggctggag tgtacagggc attaaacatc cagtctgaat tttcaattgt gactggggca
     1741 atcgtgtatc cgtggtgctt gagaaaagat tcaatctgag ttctggtttc catagattgc
     1801 ccaacccgca ggtacggatg acgaaagtac cggagcggaa gctgctttgg ctttaagagc
     1861 attttggtaa cagtctcccc ccgaatgatg tcttcggtaa aaacgtcaac tggtgtctca
     1921 tgcaaagacg gatgcgaata ggtgtggttt cccagttcca atccagcatc aacccagagt
     1981 ttgagcagct caacccggcg gtcaatttcc ccatactgga aaagctgggc ttcgttgaca
     2041 aacccaacgg ctgcaatctg gttggcacag atggtgtgaa taagccggcg ggtcattttg
     2101 tgttggagca cgacactggt ttgtcgtcca ttgagaggaa ggtcgtcaaa ggtgattgca
     2161 attttgtttg gttcgggggc aggttttatc ggtgtctggc tgagtattgg ttggggaagg
     2221 atggatagga ttgatagtaa gctgcagaat aagtgactga gttggttctt catagatggc
     2281 ctggggttgg tttgatggta tgtcgttcag ataaatattt ttgagctggg aagtgtcaga
     2341 aacgattgaa ggttttggat ttagtttctg tgtattccgt gtattccgtg gtttctttat
     2401 ctgaaattct ataatggata gtctttgagg caaggtttcg tcaagaagtc tgagagggtg
     2461 attgggaaat gggttaggga aaatactgaa aaacaggaaa aaattattga ttacaggaaa
     2521 aaattttatt aaggttagag agtaaagtta tcttattcat tatcagacgt aaattagttt
     2581 aacaaccgca gattctgtga tagatgggat tccggggaac acaatttttc cagttgtttt
     2641 ttagagtata ctttcgacgg tcttccgctc cgttcccaat tcatttcctg aaagaagttc
     2701 aaaattatgt ttcgtcactg gtttttctcc ttcctcttta ttatcagtct cgggcttctc
     2761 ctcgctgggc atacctcagt tcaggcccag tatcggcaac ttgaagaacc gcctccgatg
     2821 caaggctcac ctgaaatcct gaaaaaaatt ggaattgatc aaaaactcaa cgctcaaatt
     2881 ccactcaccc tgccttttaa agatgaaacc gggcgtgata tttccttgca agaatacttt
     2941 ggaaccaaac cggttattct ggcacccgtg tactatgaat gccccatgct gtgtaccctc
     3001 accatcaatg gcgtggttca atcgctggac cagctcaaaa ccgttgcggg aaaagatttt
     3061 ctggtggtgg cggtcagttt caatccgctt gagactcctg ac
//
LOCUS       DAOQDO010000526         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24737_length_3099_cov_3.560775, whole genome shotgun sequence.
ACCESSION   DAOQDO010000526 DAOQDO010000000
VERSION     DAOQDO010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24737_length_3099_cov_3.560775"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /gene="priA"
                     /locus_tag="PKZ53_09545"
     CDS             <1..1399
                     /gene="priA"
                     /locus_tag="PKZ53_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNJ40721.1"
                     /translation="RLDEWERIREGEARIVIGTRSAIYAPLVELGLIVVDEEHDTSYK
                     QAETPRYHGRDTAVLRAHRKGCVVVLGSATPAIESAYHASTGKYRKLELNQRFANRAL
                     PTVEVVDMREVFKRHGKQVVFSDELLLALKETQAKGEQSMVLLNRRGFSSFLLCRRCG
                     ESIPCPDCDVTLTYHKADQRLVCHYCNHQAPVPKQCPNCNGLFIYFVGEGTEQLEELL
                     QAQFPDFRIGRLDRDTTRRRGVFERTMGEFASGSLDVLVGTQMIAKGHDFPNVTLVGV
                     VSVDAGLALPDFRSGERTFQLLAQVSGRAGRGELPGRVVLQTYHPEHYALQCARTQDY
                     TQFYNREINFRKQLWYPPFSVLTNIIVRHKDATQGSTLADKFGKFLRAAAQGDNTVRI
                     LGPSEAPLSKLKQEYRYQILIKARQRRKLRDVLDLAFTYLADDAAAKTGKAFTPENNP
                     DRYHIMVEVDPVDLL"
     gene            1486..2655
                     /gene="mnmA"
                     /locus_tag="PKZ53_09550"
     CDS             1486..2655
                     /gene="mnmA"
                     /locus_tag="PKZ53_09550"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNJ40722.1"
                     /translation="MEKIAVAMSGGVDSSAVAALLKEQGQDIVGLSMQLWNQRRINVG
                     PDGEPLPSRCCSLDDLYDARSVSAHLGIPFYVLNFEEDFEEHVVAPFVASYVNGNTPS
                     PCVACNSRLKFKTLVKLAEDIGAAKVATGHYARIHFNHETGRYELLKGLDPNKDQSYF
                     LFELTQEQLAVALFPLGEMSKPEVREIARRNGLPTAEKAESQEICFIPDGNYARFIER
                     YLQEAGGEVHAEPQAPVASALVQLGLRRTLPEPGEIVGVDGTVLGHHEGTHRYTIGQR
                     RGLKINSPDGQPLYVVGIDPIRKRVIVGSGDYLPGKQYTAARMNWITIPELTEPIRVR
                     ARIRYRHGEAAATVFPEENGTVRVVFDEPQRAITPGQATVFYDGDKVVGGGWITG"
     gene            2612..3004
                     /locus_tag="PKZ53_09555"
     CDS             2612..3004
                     /locus_tag="PKZ53_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40723.1"
                     /translation="MTETKLSVVAGSRANFFGVRWLDTAFQSGGLTPSCCGVRRLDAA
                     LNDMGSWLTCQCGNLIHKNLFCGTGISIAVTEDFLNQECQNIPAQDLISQMILQMDWL
                     LQCKTCGRLIILNEKENGKLEFYCKEDL"
BASE COUNT          753 a          797 c          848 g          701 t
ORIGIN      
        1 acgactcgac gaatgggaac gcatccgcga aggcgaagcc cggattgtga ttggcacccg
       61 gtcggcaatc tatgcgccgc tggttgaatt aggattgatc gtggttgatg aagaacacga
      121 cacctcatac aaacaagctg aaacaccacg ctatcacgga cgtgatacgg cagtgctccg
      181 ggcacatcgc aaaggctgtg tcgttgttct gggaagtgcc acgccagcca tcgaatcagc
      241 ctaccatgcc tcaaccggta aataccgaaa actcgaactc aatcaacgct ttgccaatcg
      301 agcgctccca acagtcgaag ttgtggatat gcgcgaagtc tttaagcgcc acggcaaaca
      361 ggttgtgttt tccgatgagc tcttgctggc actcaaagaa acccaggcca aaggcgaaca
      421 aagcatggtg ctgctcaacc ggcgaggatt ttcaagcttt ctgctctgcc gtcgctgcgg
      481 agaaagcatt ccgtgcccgg attgcgacgt gacactgacc taccacaagg ctgaccagcg
      541 gctggtgtgc cattactgta accatcaggc accagtcccc aaacaatgcc caaattgtaa
      601 cggcttgttt atttactttg tcggagaagg caccgaacaa ctcgaagagc tgctgcaagc
      661 ccagtttcct gactttcgaa ttggccgcct tgaccgggac accacccggc ggcggggtgt
      721 atttgaacga accatggggg aatttgcttc aggaagtctg gatgtgctgg tcggaaccca
      781 aatgattgcc aaagggcacg attttccaaa tgtcacgctc gtcggcgtgg tctcggttga
      841 tgccgggctg gcgctgccgg atttccgctc tggcgaacgc acgtttcagt tgctggctca
      901 ggtgtctggc cgggctggcc ggggtgaatt accaggccgt gtggtcctgc aaacctatca
      961 tccagaacac tatgcgttgc agtgtgcccg aactcaggat tacacccagt tttacaatcg
     1021 ggaaatcaac ttccggaaac aattgtggta tccgccgttt tcagtcctga ccaatattat
     1081 tgttcgccat aaagacgcca cccaggggtc aacactggcc gataaattcg gaaagtttct
     1141 ccgggctgcc gctcagggcg acaacaccgt acgcatcctg gggccatcag aagcaccact
     1201 ttcgaaactc aagcaggagt accggtatca aatcctgatt aaagcccggc aacgccgtaa
     1261 gttacgggat gttctcgatc tggcgtttac ctatctggct gatgatgcgg cagccaaaac
     1321 cgggaaagcc ttcacacctg aaaataaccc tgaccggtat cacatcatgg ttgaagttga
     1381 tccggttgac ctgctttgag aaatgagaat caaggacgta gaatgaaccc ccgcccgcta
     1441 acgcaggtgg tactgacttc attcccagtt agatagttgg acatcatgga aaagattgca
     1501 gttgcgatga gtggtggagt ggatagctct gccgtagcgg cgttgctcaa agagcagggc
     1561 caggacattg ttggcctttc gatgcagttg tggaatcagc ggcgcatcaa tgtcggaccg
     1621 gacggcgaac cgctcccttc gcggtgttgt tcgctcgatg atttgtatga tgcgcgttca
     1681 gtctcagccc atcttgggat tccgttttat gtgcttaatt tcgaagagga tttcgaagag
     1741 catgttgtag ccccgtttgt cgccagttat gtcaacggca acacgccaag cccctgtgtg
     1801 gcctgcaaca gccggctcaa atttaaaacg ctggtcaaac tggccgaaga cattggcgcg
     1861 gccaaagtgg cgacgggtca ctatgcccgc attcacttta accatgaaac cggacgttat
     1921 gagttactca aggggctgga tccaaataaa gatcaatcct actttttgtt tgaactgacc
     1981 caggagcaac tggccgtcgc tctctttccg cttggtgaaa tgtcgaaacc cgaagttcgc
     2041 gaaattgctc gccgcaatgg acttccgacg gctgaaaaag cagagagcca ggaaatctgc
     2101 ttcattccag atggcaacta tgcccggttt atcgagcgct atctgcagga agcaggtggc
     2161 gaagtccacg ccgaaccaca agcccccgtg gcatcagccc tggttcaact ggggttacgg
     2221 cgaacgctcc ctgagcccgg tgaaatcgtt ggcgtggatg gcacggtgct tgggcatcac
     2281 gaaggcacgc accggtacac gattgggcag cggcgcggac tgaaaatcaa ttcgccagac
     2341 ggtcagccgc tctatgtggt gggcattgat ccaattcgca aacgggtcat tgtggggtca
     2401 ggcgattacc tgcctggaaa acaatacacg gcggcccgga tgaactggat cacgattcca
     2461 gaactgactg aaccaattcg ggttcgagca cggattcgtt accggcacgg ggaagcggca
     2521 gccacggttt tcccagagga aaatggcaca gtgcgcgttg tctttgacga accacaacgc
     2581 gccatcacgc ctggtcaggc gaccgtcttc tatgacggag acaaagttgt cggtggtggc
     2641 tggatcacgg gctaactttt ttggagtgcg gtggcttgac accgcttttc aatcgggcgg
     2701 cttgacgccc tcttgctgtg gagtgcggcg gcttgacgcc gctttaaacg atatgggttc
     2761 ctggttgacc tgccagtgtg gcaacctgat acacaaaaat ctgttctgcg gaactggtat
     2821 ttcgattgcc gtaaccgaag actttttgaa tcaagaatgt caaaatattc cggctcaaga
     2881 tcttatcagc cagatgattt tgcaaatgga ttggctcttg cagtgcaaaa cctgtgggcg
     2941 gctgattatt ttaaacgaga aggaaaacgg caaacttgag ttttactgca aagaagacct
     3001 gtagccagca aatcgattgg aggaggaaag actatgtcaa acattgcaac cttaccggtt
     3061 gagcctccct gggaaagtga ttatgaattg gatgaacag
//
LOCUS       DAOQDO010000527         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24755_length_3098_cov_2.897798, whole genome shotgun sequence.
ACCESSION   DAOQDO010000527 DAOQDO010000000
VERSION     DAOQDO010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24755_length_3098_cov_2.897798"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..424)
                     /locus_tag="PKZ53_09560"
     CDS             complement(41..424)
                     /locus_tag="PKZ53_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ40724.1"
                     /translation="MSRQTIHTEHAPKAIGPYVQAVRIGQFVFTSGQIAIDPATSQVV
                     EGDVSAQTERVLTNITAVLEAAGASLKNVVKTTVFLKDMNHFQAMNEVYGRFFTENPP
                     ARSTVEVARLPKDVLVEIETIAWVG"
     gene            780..1196
                     /locus_tag="PKZ53_09565"
     CDS             780..1196
                     /locus_tag="PKZ53_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40725.1"
                     /translation="MSHPQLSTRSIDELVRTFEDGTLDPDTFSHADHLRVALFYVATL
                     PFEQARDQFRSTLLNFVKIHNISVYSETVTQFWMKKVFTFWHQNHETLTSSVLEAELV
                     RQLAHSKAIQRHFSPELLATERAKTEWVEPDREPIA"
     gene            1324..>3098
                     /locus_tag="PKZ53_09570"
     CDS             1324..>3098
                     /locus_tag="PKZ53_09570"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40726.1"
                     /translation="MSHAGCRLLKTTFLTVIALELMVCIGLGAWLTSQAAVYPSSSQA
                     KKSILIAGREESFSFEQMPIQSYTVDLTAGQLADIRVIQKGIDIQIKVTAPDGSLVVN
                     ANRMNALNGEEPVYILATAPGLYCLDISPVQGIAPGSSCLIQLLDVRQPTETDQARLQ
                     AQTYFAEAEAHFATGTAQGLQDAISSYGKAIPLYHQTGDRYGEANAVTNIGSMSMLLR
                     NLEQAVSYFERAVPMWENLNDYNSLTVTLTQLGQILLSNGQYEKARLNFERGLELSRQ
                     HQDRQTEGFSLYSLGLVQNALGDFESALSYFREAETIFDSLSLHQLKAEAKFRIGENY
                     FCLGEAPEAIKPLEDAIEMFKELKNRRYHSIALVVYANVFRVLGDYQKAFTVLQEALE
                     IKKQIGDQSGQAFVLNSLGLLYATIGDKSKALNCYQQALAIREAQKDLPGQASTLHNL
                     GNLCFIENKYAEALTLLQQSLVISKALNNTRLQLQTQELIGIVLLALEKNLEAEQIFQ
                     QTLSQAIELKQQDQLGGLYLQLGQIAYDKEDWKKASQWYELAYKATEDCPSPIEQAVV
                     LLGLARSEVPQGHYQHARELGLKSVA"
BASE COUNT          785 a          718 c          772 g          823 t
ORIGIN      
        1 ggaacccgga accctgtttt tcgaccctga accctaaatc ctacccaacc caggcgatgg
       61 tttcaatttc caccagcaca tctttgggta atctggcaac ttcgacggtc gaacgggctg
      121 gcgggttttc cgtgaaaaac cggccataga cttcattcat agcctgaaaa tggttcatat
      181 ctttgagaaa aaccgtggtt ttgacgacat ttttcaggct ggcaccagca gcctcaagca
      241 cagccgtgat gttggtcagc acccgttcgg tttgggcgga aacgtcgcct tcaaccactt
      301 gactggtagc cggatcaatg gcgatctgac ctgaagtaaa tacaaactgg ccgatacgaa
      361 ccgcctggac ataggggcca atcgcttttg gcgcgtgttc ggtatgaatg gtttgacgtg
      421 acataaactt ggttttcgtc tcctgatggt tttgatttcc gccgaggcgg ctgcctgcca
      481 ctttaaattg tgcggtggaa gattggaact ggtttgggat tccgggctcc gggttttcga
      541 aatgggtgca gttcaaggta acagcgccat cactccattt tgttcagtcg gcagattttt
      601 tcattgacaa ttctggcttc actgtggttt tttaaggctt caccgcgcaa ggcccatggc
      661 cttgcgcggt ttttcttttg cccggcagat tttgccgggc aggttacttt tttggtactg
      721 tgccgtcggc gccaatagaa gcgctggcgg cctttttctt tgtggaaagg aatttttcta
      781 tgtcgcaccc ccagctttca actcgcagca ttgatgaact ggttcgaacg ttcgaagacg
      841 gcacccttga ccctgacacc ttcagccatg ccgaccattt gcgcgtggcg ttgttttatg
      901 tcgcgacgct gccgtttgag caggctcgtg accagttccg atcaacactg ttgaatttcg
      961 tcaaaattca taacatttct gtctacagcg aaaccgtgac ccagttttgg atgaaaaagg
     1021 tttttacgtt ttggcaccaa aaccatgaaa ccttgacgag ttctgtcctg gaagccgaac
     1081 ttgtgcgcca gttggctcat tcgaaggcga ttcagcggca tttcagcccg gaattgctgg
     1141 ccaccgaacg cgccaaaacc gaatgggttg aaccagaccg cgagccgatt gcataaatta
     1201 ttcagtgttc aagctttagc ttgccaaatt cttcaaattc aggctaagtt tagagttcct
     1261 caaacggaac tctgaaccgt caaaatcatt cgttttcagc cgcattgtgg aggtgagaaa
     1321 gacatgtctc acgcaggttg tcgtttgctc aagactacgt tcctgactgt gattgcactg
     1381 gaacttatgg tctgtattgg gcttggtgct tggttgacca gtcaggctgc tgtttaccca
     1441 tcatcaagcc aggctaaaaa atcaatcctc attgctggtc gtgaagaatc attttcgttc
     1501 gaacaaatgc ccatacagtc gtacacggtt gacctcacgg caggtcaatt ggctgatatt
     1561 cgtgtgatcc aaaaagggat tgatattcag atcaaagtca cggcgccgga tggttcgctg
     1621 gttgtcaacg ccaatcgaat gaatgccctc aatggcgaag agccagttta cattcttgcg
     1681 actgcgccgg ggctgtattg tctggacatc agtcctgttc agggaattgc accaggaagt
     1741 tcctgtttga ttcaattgct tgatgtccga cagccaacgg aaaccgatca ggctcgcttg
     1801 caggcacaaa cctattttgc tgaagctgaa gcccattttg ccaccggaac ggctcaggga
     1861 ttacaagacg ccatttccag ttacggaaaa gccattcctt tgtatcacca gacaggtgat
     1921 aggtatggtg aggccaacgc tgtgaccaat atcggaagta tgtccatgct gttgcgtaat
     1981 ctggaacagg cggtaagtta ctttgaaagg gcagttccga tgtgggaaaa cctcaatgat
     2041 tacaacagct tgacagtaac gctgactcag cttgggcaaa ttttgctgtc aaacggtcaa
     2101 tatgaaaaag cacggttgaa ttttgaacgc gggcttgaac tgagtcgcca gcatcaggat
     2161 cgtcagaccg aagggttttc gctgtattca cttgggttgg tgcagaatgc tttgggcgat
     2221 tttgagtcag cgctcagcta ttttcgagag gcggaaacca tctttgactc acttagtctg
     2281 caccaactta aagctgaagc taaattcaga atcggagaaa actatttttg tttgggagaa
     2341 gccccagagg caatcaaacc tttggaagat gccattgaaa tgttcaaaga gttgaaaaat
     2401 cgtcggtatc actcgatagc gctggtggtt tatgcaaatg tctttcgtgt gttaggcgat
     2461 taccagaaag cttttaccgt cttgcaggaa gcgctggaaa tcaaaaaaca aattggagat
     2521 cagagcgggc aggcatttgt tttgaactct cttggactgc tgtacgcaac cattggcgat
     2581 aaatcaaagg cactgaattg ctatcaacag gcactggcta tccgtgaagc tcagaaggat
     2641 ttacctggac aggcttcaac tctccacaac ctgggtaatc tctgttttat cgaaaacaaa
     2701 tatgccgagg cgctgacatt gttacagcaa tcgctggtca tttcaaaagc actcaataat
     2761 acccggttgc aactccaaac ccaggaattg attggaatag tgttgcttgc actggaaaaa
     2821 aacctggagg ctgagcaaat ctttcaacaa acattgagcc aggcgataga actcaaacaa
     2881 caagaccagc ttggaggctt gtacctgcaa cttggccaga ttgcctatga caaagaagac
     2941 tggaaaaagg ccagccagtg gtatgaactg gcttacaaag caactgaaga ttgtccatca
     3001 ccaattgaac aggcagttgt attgcttggt ttagccagga gtgaggttcc gcaggggcat
     3061 taccagcatg cccgggaatt gggtttgaaa tcagttgc
//
LOCUS       DAOQDO010000528         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24882_length_3088_cov_3.805473, whole genome shotgun sequence.
ACCESSION   DAOQDO010000528 DAOQDO010000000
VERSION     DAOQDO010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24882_length_3088_cov_3.805473"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..386)
                     /locus_tag="PKZ53_09575"
     tRNA            complement(310..386)
                     /locus_tag="PKZ53_09575"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:His,seq:gtg)
     gene            complement(576..2789)
                     /locus_tag="PKZ53_09580"
     CDS             complement(576..2789)
                     /locus_tag="PKZ53_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNJ40727.1"
                     /translation="MSYRLEQTSFPKILCECHKQRLSGTLSINHNSINKSVYFEDGAT
                     VYASSDIISERFGERLRMSGKLSPQQFEEAILCMHLSQHQLGECLIDLGYLQEAEVKT
                     LLTEQVTQIIFSLFTWQEGQYDFKPGRIEIKGFQLAMPTAEIIFEGIRQLPDMQLLRR
                     WLGDFNRKVVPASDPYMLFQTVSLKPHEAYVISRMDAPLSINELLAISGIPEEQLLRT
                     ICALKMAGIVESLEGDTRPQLFANSVAESMQAPSDAQFQSVDAAAAAQLCFEVTEVVR
                     QIEQNADHYQVLGVSRRATPEEIKKAYRERAKKFHPDRHSQLAAFDFQIKSELERVFI
                     RVQEAYGTLGDEAKRRKYDAAVRFPTTPTAPPSSGVSTTAPRPPVTPSTTGSRPTSTF
                     TPTPTNPPPLRPAPPPPGPNVPYSSSNPYQNPAPRPSVPPASSYPPPARPPAPPAGTY
                     PPAPPAGQRPATPPGQPQYNTPPPVQPPPTGQRPAPPPGQPQYNTPPPVQPPPAGQRP
                     PTPPGGQPQYSTPKPFQSPPSGPNPVSTPGPSSTYPPPSATYPPQSRQPSGSYPPAGR
                     TTPRSGTPSPLPPSQTSGITPNLSASEYYMRCIEYLGNNDLEKAYQSIRRSVEMRPKN
                     SEYHAQLARVLMKMQGRNKQAEKEYLVAIELDAENPDLPDLYVELSDLYRKFGMELKA
                     NEMLGKALEINPDHYEAKEKAEKYTPPPEPTWKKASGRFGSLVGKVFHKGDKGGS"
BASE COUNT          729 a          733 c          873 g          753 t
ORIGIN      
        1 tccagctcca ttggcaccaa tataaccaaa gatttctccg cgttgaactg aaaaactgat
       61 gttcttgagc actggctggg tgccaaattg tttgctgatg tcctggagtt gaagaactgg
      121 gggcgatgca atatcaaccg ctccagattg ggcaaccggg gaaaaatcca tcataaaggc
      181 agccgacgtt ggaaaaattg cgtgccagca tgtgggttga gtgaatgctg gatttgagga
      241 ggaacggatc ttccagaaat gaaaagggcc acgcggcagt gtgcgcgtag ccctgatcca
      301 aattccaagt ggggcgattg atgggtctcg aacccacgac ctcctgggcc acaaccaggc
      361 gctctaacca actgagctac aaccgccata aaaagggaaa gcggcacaat agcgcaggtt
      421 ttgaaaaaac gcaatcagaa tgtatctgag cgggagccgt actttggggc tgaaggaaac
      481 aatccaaaaa ttcgcaaacc caggattgcc agggattgga ctgaaaaggt tgcgctttgg
      541 gcgaggtcca aaaccgttca gttacactcc aggggctaac ttccaccttt atcgcctttg
      601 tgaaagactt ttccaaccag cgaaccaaac cgtccactgg cttttttcca ggttggttcg
      661 ggtggcgggg tatacttttc agctttttct ttggcttcat aatggtctgg attgatttcc
      721 agagccttcc caagcatttc attggccttg agttccatcc cgaatttgcg atagaggtcc
      781 gagagttcaa catacaaatc tggcaagtca gggttttctg catccagttc aatggccacc
      841 aaatattctt tctcggcctg tttgttgcgg ccttgcattt tcatcagcac acgggccagt
      901 tgggcatggt attcagaatt cttcggacgc atctcaacag accgccggat tgattgatag
      961 gctttttcaa gatcattgtt ccctaaatat tcaatacacc gcatgtaata ttctgaagca
     1021 cttaaatttg gagtgatacc gctggtctgt gatggcggta aaggactcgg cgttccactg
     1081 cgcggtgttg ttctgccggc tggaggatag ctaccgctcg gctgacggga ttgtggcgga
     1141 taggtcgctg acggaggcgg gtaagttgat gaaggtcccg gcgtcgaaac aggatttggc
     1201 cctgacgggg gagactgaaa cggctttggt gtgctgtact gaggctgtcc gcctgggggc
     1261 gttggcggtc gctgcccggc tggcggcggt tgaaccggag gcggggtgtt gtactgaggc
     1321 tgccctggcg gtggcgctgg tcgctgcccg gtcggtggcg gttgaaccgg aggcggggtg
     1381 ttgtactgag gctgccctgg cggtgttgct ggccgctgcc cggctggcgg cgcgggtgga
     1441 taggttccgg ctggaggggc tggcggtctg gctggtggcg gataggaaga agctggtgga
     1501 acagagggac gaggagcagg gttttggtac ggattcgaag acgaatacgg tacatttggt
     1561 ccaggagggg gcggcgccgg gcgcaagggc ggtggattcg ttggggttgg ggtaaacgtc
     1621 gaggttggtc ggctccccgt tgtcgaggga gtgactggtg gtcgtggtgc ggttgtggat
     1681 acacctgatg acggcggtgc cgttggggtc gtcggaaaac gtacggcagc atcatatttg
     1741 cgccgcttgg cttcatcacc taatgtgcca taggcttcct gtacccgaat aaaaactcgt
     1801 tccagttcag atttaatctg aaaatcgaaa gcggccagtt gcgaatggcg atctgggtga
     1861 aactttttgg cccgttcacg atacgctttt ttgatttctt ctggtgtagc ccgacgactg
     1921 acacccagta cctggtagtg gtcagcgttc tgctcaattt gacgaaccac ctcagtcact
     1981 tcaaaacata actgggccgc tgccgccgca tcaacactct ggaactgggc atcagacggt
     2041 gcttgcatgc tctcagccac tgaattggca aacagttgtg gtcgggtatc cccttccagg
     2101 ctttccacaa tacctgccat tttcagagca caaatcgttc gcaacaattg ttcttcagga
     2161 atgccagaaa tggccagtaa ctcattgata ctgagcgggg catccatgcg tgaaatgaca
     2221 taggcctcat gaggcttcag tgacacagtt tggaacagca tgtagggatc agacgccggc
     2281 accaccttgc gattaaaatc tcccaaccat cgccgaagca gttgcatatc tggaagttgc
     2341 cgaatgcctt caaaaataat ttcggcggtt ggcatcgcta actgaaaccc tttgatttca
     2401 atccggccag gcttgaaatc atattgccct tcctgccagg taaagagcga aaagatgatt
     2461 tgggttacct gctcagtcag caaggttttg acttcagctt cctgcaaata accaaggtca
     2521 atcagacatt cgccaagctg gtgctggctc aggtgcatac agagaatcgc ttcctcaaac
     2581 tgttgcggag aaagcttccc agacatgcgc agccgctcgc caaatcgctc gctgatgatg
     2641 tcgctactgg catagaccgt ggcgccatcc tcaaagtaaa ccgacttgtt gatggaatta
     2701 tggttaattg acagagttcc gctgagccgc tgtttgtggc attcacacag aatcttcggg
     2761 aaagacgtct gctccagtct gtaagacatc ctctgagcct cccaatgcaa ttcgtaaaga
     2821 attgctcctt tgcctgatac aaatatgacc tgtgtgcggg attgataacg aacgaacttt
     2881 gatgaccagt gacatgttat ctactcaacg gacaaccctc tgaacaggag aaatcaagtg
     2941 ggctgtctgg ccaaactcct ctgaagggag cttctcaatc aaaaagtgag tttgattgtg
     3001 gcagagatgt ttgagtgtgt gcaaaacaga aagagcgcac atgaatacag aaccctacca
     3061 gatatttcca ggagaattca actctaaa
//
LOCUS       DAOQDO010000529         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_24916_length_3085_cov_3.118152, whole genome shotgun sequence.
ACCESSION   DAOQDO010000529 DAOQDO010000000
VERSION     DAOQDO010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24916_length_3085_cov_3.118152"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..2393
                     /locus_tag="PKZ53_09585"
     CDS             6..2393
                     /locus_tag="PKZ53_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNJ40728.1"
                     /translation="MAETSSSGVAPSVSKKSASPVAEPQPAAGKAKKPVLRQVSKEEG
                     LSEGDILKELYGDLKNDTSSAHQAAKPQPVAPVAARPTTPEFEPLLTPPPQIVQSSPA
                     PAEVKPEPAAPQTPASARKPKHISSLNEVVRPEPVVSPPNFAVPSTGDFDTPGSATRR
                     LLREKLAQKQRQNLQSFTDEPVQPVFEPPPTPTPRRPTGGSDTRQLIAEAKAAAEARQ
                     AAEARQAAEVRPIFDPMPLAKARPDPEIEKMVGSAHIVRHQTGEHRRDTLERAKSSML
                     ASTTPKPPPSMTLGFGGRSAISSSGGYQKPSILMLEMPPPRQEQEDAELWDRARLLAE
                     KCREFNVTGKIENIHPGPVVTTFEFKPDPGVKYSRVANLTDDLCLAMKAVSVRIDRLP
                     GKSTIGIEIPNLKREVIFLREVVESDDYERKHRDSGFSPLMIALGKTIEGNPYASDLA
                     KMPHLLIAGSTGSGKSVMVNALICSLLLQATPSDVKMILVDPKRVELGLYEGIPHLLT
                     KIIVDPKCAANALNWAVREMETRYKQLANFGVRNISQFNNEICGSGDVARFGLDEPPA
                     KLPYLVIVIDELADLMMVASSEVETAITRLAQMARAVGIHLVVATQRPSVDVITGLIK
                     ANFPARMSFRVSSKVDSRTILDANGAEGLLGQGDMLFLPPGTSRLERIHGSYVSEAEI
                     NRIVDHVKAQGQPQYNDSIQRSEDEGEGGENSLGERDELYDDAMRIIVQMGRASTSVL
                     QRRLSIGYGRAAKILDMMEIEGYVGPSEGSKPREVKRLAVEYVEMLDGHRLNGDD"
     gene            2380..2967
                     /locus_tag="PKZ53_09590"
     CDS             2380..2967
                     /locus_tag="PKZ53_09590"
                     /inference="COORDINATES: protein motif:HMM:NF017517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNJ40729.1"
                     /translation="MATTKSRWAEENGLEKSTSLQNASSMNIPLSLRVYWVHSVQPNL
                     AIMPCPEGGAQLESEIEALQLSGVNVLVSLLVPEEEPRLQVETEAILCQQKGLRFISF
                     PIPDFDIPTSFQDVDQLVVQLQAELLAGRKVVIHCRGGIGRSGMVAACLLIKQGISVS
                     DSLTALRTARRCAVPATRAQANWIEKYNEWRILSE"
BASE COUNT          763 a          822 c          804 g          696 t
ORIGIN      
        1 cagacctggc tgaaacttcg tcatctggcg ttgcgccatc cgtgtctaaa aaatccgcgt
       61 caccagtagc tgagccgcag cccgcagccg ggaaggcgaa aaagccggtt ttgcggcaag
      121 tttcgaaaga agaaggactc agcgaagggg atattctcaa agaactgtat ggtgatctga
      181 aaaacgacac cagtagtgcc catcaggctg ccaaacccca gccggtggcc cccgtggctg
      241 cccgaccaac gacccctgag tttgagcccc tgctcacgcc gccacctcag attgtgcaat
      301 caagcccggc gccagccgag gtcaaaccag agcctgccgc accccaaacg ccagcttcag
      361 ctcggaagcc caagcatatt tcttcgctta atgaggtcgt gcggcccgaa cccgtggtgt
      421 ctccgcccaa ttttgcggtt ccatcaaccg gagattttga tacacccggt tcggcaacgc
      481 gccgattact gcgtgaaaag ctggcccaga agcagcgtca gaatttgcaa tccttcaccg
      541 atgaaccagt tcagccagtg tttgagccac cacccacccc gacgcctcgc cgtccgactg
      601 gtgggtctga tacacggcaa ctgattgccg aagccaaagc tgctgccgaa gctcgccagg
      661 cggctgaagc ccgtcaggcc gccgaagtcc gaccaatatt tgacccaatg ccactggcga
      721 aagccaggcc agaccccgaa atcgaaaaaa tggttggttc cgcgcatatt gttcgccatc
      781 aaacaggtga acaccggcgt gacacgctgg agcgggccaa atcttcaatg cttgcatcca
      841 ccacacccaa accaccgccg tcaatgaccc ttgggtttgg tggcagatcc gcaatttcat
      901 catcaggggg gtatcaaaag ccttcgattc tgatgcttga aatgcctccg ccccggcagg
      961 aacaggagga cgctgaattg tgggatcgcg cccggttact ggctgaaaaa tgtcgtgaat
     1021 tcaatgtcac cggcaagatt gaaaacattc acccaggccc agtggtgacc acctttgagt
     1081 tcaaacccga tccaggcgtg aaatacagcc gggtggccaa tttgaccgat gacctgtgtc
     1141 tggcgatgaa agccgtatcg gtccgaattg atcggctccc aggtaagtca acgattggga
     1201 ttgaaattcc caatctcaag cgcgaagtga ttttcttgcg tgaggtggtt gagtcagatg
     1261 actatgagcg aaagcaccgg gactctggtt tttcaccttt gatgattgcc cttggaaaaa
     1321 caatcgaagg gaatccgtat gccagtgatt tggccaaaat gccccactta ttgattgctg
     1381 gttcaaccgg ttccgggaaa tcggtgatgg tcaatgccct gatttgctca ctgttactcc
     1441 aggccacgcc ttcggatgta aaaatgatcc tggttgaccc gaaacgggta gaacttgggc
     1501 tctatgaagg aattccgcac ctgttgacca aaatcattgt agatccaaaa tgtgcggcga
     1561 acgccctcaa ctgggccgtc cgggagatgg aaacccggta caaacaactg gccaatttcg
     1621 gagtccgcaa tatcagtcag ttcaacaatg aaatctgtgg ttctggcgat gtcgcccgct
     1681 ttgggctgga tgaaccaccc gccaaattgc cctatctcgt gattgtgatt gacgaactgg
     1741 ccgacctgat gatggtggct tcatccgaag tggaaactgc gattacccga ctggcccaaa
     1801 tggcccgtgc cgttggaatc catctggtgg tggctactca gcgaccctca gtggatgtca
     1861 tcaccgggtt gatcaaagcc aatttcccag ctcgaatgtc attccgggtc tcatcaaaag
     1921 ttgactcacg aaccattctt gatgccaacg gggccgaagg cttacttggc cagggcgata
     1981 tgctgttcct tccgccaggg acatctcgcc tggaacgcat ccacggttcc tatgtcagtg
     2041 aagccgaaat caaccgcatt gttgaccacg tcaaagcgca agggcaacct cagtacaatg
     2101 acagcattca gcgttcggaa gatgaaggcg aaggtggtga aaacagtctg ggcgagcggg
     2161 atgaactcta tgacgacgcc atgcgaatca ttgtccagat gggacgggca tcaacgtcgg
     2221 ttttgcaacg gcggctgagc attggctatg gtcgcgccgc aaaaatcctc gatatgatgg
     2281 aaattgaagg gtatgtcggg ccgtctgaag gcagcaaacc gcgtgaagtc aaacgccttg
     2341 ccgtcgaata tgtcgaaatg ctggatggcc accggctcaa tggcgacgac taaatccaga
     2401 tgggctgaag aaaacgggct ggagaagtca acctcacttc aaaacgctag ttcaatgaat
     2461 attccgctct cactccgagt ctattgggtt cattcggttc agccaaatct ggctatcatg
     2521 ccctgtcctg aaggtggtgc tcaacttgaa tctgaaattg aagcgcttca attgagtggg
     2581 gttaatgttc tggtttcatt gttggtgccg gaggaagaac cacgattgca agttgaaact
     2641 gaagcgatac tgtgtcagca aaaaggactc cggttcatct catttccaat ccccgatttt
     2701 gatattccga cttcttttca agacgtggac cagcttgtgg ttcaacttca agcagaacta
     2761 ctggctggtc gtaaagttgt gatccattgc cgaggtggga ttggtcgttc gggaatggtt
     2821 gcggcctgtc ttctcatcaa acagggaatt tccgtatccg acagcctgac tgcgcttcga
     2881 actgctcgcc ggtgtgcggt cccagccacc cgcgcacagg ctaactggat agaaaaatat
     2941 aacgaatgga gaatcctaag cgagtagcga atcctgctag ctttgtctga ctcctcagta
     3001 ctcataccat ttgtctctgg aatgaaatct tcgcattatg gaaaggctaa aatgttgaaa
     3061 atattgtatt ttcttcagcc cttag
//
LOCUS       DAOQDO010000530         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25003_length_3079_cov_3.096230, whole genome shotgun sequence.
ACCESSION   DAOQDO010000530 DAOQDO010000000
VERSION     DAOQDO010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25003_length_3079_cov_3.096230"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..976)
                     /gene="xerC"
                     /locus_tag="PKZ53_09595"
     CDS             complement(59..976)
                     /gene="xerC"
                     /locus_tag="PKZ53_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNJ40730.1"
                     /translation="MEQYLEDFKKHLMYERNVSEHTLRNYLSDLEQFHDYLCPADKQG
                     VRREVNIRDIDNITIREYMATLYSKNKKKTSIARKLATLRTFFKYLCREGILEMNPAR
                     LVSSPRLEKKLPNYLTVEEVIRFIEIPDLETVLGKRDRAILEMLYGTGVRVSELVNLN
                     LQDIDFTQMTVRVRGKGRKERIVPFGEYAKKAVELYLGVRGELMMNAPEDKRVPNAVF
                     YNYQGTRITTRSVGRMIDKYIKECADLHHISPHSLRHSFATHLLNAGADLRSIQELLG
                     HARLSTTQQYTHISQDRLMEVYDKAHPKA"
     gene            1115..2008
                     /locus_tag="PKZ53_09600"
     CDS             1115..2008
                     /locus_tag="PKZ53_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNJ40731.1"
                     /translation="MAKPWEIPDLHPGMHLDTAARHVILARFEQMASYRDAVLEDTDI
                     ENLHDMRVCSRRLRAAWRSFGTLFPGKRIARFQREARDITRTLGTVRDLDVMLESLEK
                     AERRAPGELLDGFDSFYGYIGSIRAEEFELLTTALQKWHPTQFIEFFDQAESDPFAHA
                     VLFEEQAHVVLSALAHEFLSHQPCLADPTASEEQHIMRIAGKRLRYAIEIFASCLSNA
                     GQLLHILKTVQEDLGTLHDCDVMVEFLNYRQRVCLTLERPTILWLIEYYRNRRETTYG
                     NLVTGWHKAVDEGFSNLLLAL"
     gene            complement(2062..2943)
                     /gene="prmC"
                     /locus_tag="PKZ53_09605"
     CDS             complement(2062..2943)
                     /gene="prmC"
                     /locus_tag="PKZ53_09605"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNJ40732.1"
                     /translation="MSESPTLKILLREGTALLRAAKIPAPHQTASLLVCGILKLDHTT
                     LIAHPEYTVSAVDATRLRDAFSRRAQGEPAQYLIGLQEFYGLEFKVSPAVLIPRPETE
                     FLVEVALEFCRHQNLTCPRIIDIGTGSGCLAVTLAVKLPAAQVLALDLSPDALAIARQ
                     NAVTHCVADRIQFLESNYLSAVRDLPPAERVDVVVTNPPYISQAEYADLQREVRDFEP
                     KMALVGGEAGTEGYAQILADLDRVLKPDGLFACEVGYTQAQAVAQLGQQAGWDDQRII
                     DDLQGIPRVVVLRKQPL"
BASE COUNT          714 a          788 c          795 g          782 t
ORIGIN      
        1 tcattcttca ttcttcactc ttcattcttc attcttcatt cttcattctt cattccgttc
       61 aggctttcgg atgggctttg tcatagactt ccatcagccg atcctgtgaa atgtgggtgt
      121 attgctgggt ggttgacagc cgggcgtggc caagcaattc ctggatggaa cgcagatcgg
      181 cgccggcatt gagcaggtgg gttgcaaacg agtgccgcaa actatgtgga ctgatgtggt
      241 gcaggtcagc gcactctttg atgtacttgt caatcattcg cccaacggag cgcgttgtga
      301 tgcgcgttcc ctggtaattg taaaagaccg cattcggtac gcgcttgtct tccggcgcat
      361 tcatcatcag ttcgccgcgc acgccaagat agagttccac cgcttttttg gcatattcac
      421 caaatgggac aatgcgttct ttccgacctt ttcctcgtac acggactgtc atttgggtga
      481 agtcaatatc ctgcaagttc aaattgacca gttccgagac gcgaacacca gtcccataca
      541 acatttccaa aatggcacga tcccgcttgc cgagtacggt ttccaaatct ggaatttcaa
      601 taaagcggat gacttcttca accgtgagat aattcggaag ttttttctca agccggggcg
      661 acgaaaccag ccgggccggg ttcatttcca ggattccttc gcggcataag tatttgaaaa
      721 aggttcggag tgtggcgagt ttgcgggcaa ttgaggtctt ctttttgttt ttgctataca
      781 gcgtcgccat atactcgcga attgtgatgt tatcaatatc gcggatattg acttcacggc
      841 gaacaccttg tttatcagcc gggcagagat aatcatggaa ttgttccaaa tcgctcaggt
      901 agttgcgcaa ggtatgttct gaaacgttgc gctcgtacat cagatgtttt ttgaagtcct
      961 ccaaatactg ctccattgca atcctccggg aaggccgaat gtcgaatgtt tgatttagaa
     1021 tgtagaatgg aaaccccacc cgctcacgca ggtggtactg acactgcatt cttcattctt
     1081 cattcttcat tttcaactcc tcattcttct ttctatggca aaaccttggg aaatccctga
     1141 tttacatcca ggcatgcacc ttgatacggc ggcacggcat gtgattctgg cccgctttga
     1201 acagatggct tcgtatcggg atgcggttct ggaagacact gacattgaaa acctgcacga
     1261 catgcgggtg tgttcgcgcc gattacgggc ggcgtggcgc agtttcggca cgcttttccc
     1321 cggaaaacgc atcgcccgct ttcaacgcga ggcccgtgac attacccgca cactgggcac
     1381 ggtccgtgac cttgatgtga tgctcgaaag tttggagaaa gccgaacgtc gggcaccagg
     1441 cgaattgctc gacgggttcg attcatttta tggatacatt ggctcaattc gagctgaaga
     1501 atttgagtta ctgaccacag cccttcagaa gtggcatccc actcagttta tcgaattttt
     1561 tgatcaggcg gaatctgacc cgtttgccca cgcggtcctc tttgaagaac aggcccacgt
     1621 tgtcttgtcg gccctggccc acgaattctt gagccatcag ccgtgtctgg cagatccaac
     1681 tgccagcgaa gaacaacaca tcatgcgcat tgcgggcaaa cgattgaggt atgccattga
     1741 aatctttgcc agttgcctct ccaatgccgg acaattactc catattctca agacggtgca
     1801 ggaagacctg ggaacgctcc acgattgcga tgtgatggtg gaattcttga actaccggca
     1861 gcgtgtctgc ctcacattgg aacgtccgac cattttgtgg ttgattgagt actatcggaa
     1921 tcgccgggaa acaacctacg gcaatttggt gacaggctgg cacaaagcgg tggatgaagg
     1981 attttccaat ctgctactgg cgctgtaaca cctggggctg caaaatcctc gggcctgggg
     2041 cttggggctg aaaaatccgg gctacagtgg ctgtttcctg agaaccacca cacgtggaat
     2101 tccctgcaaa tcatcaatga tgcgctggtc atcccaaccg gcttgttgtc cgagttgagc
     2161 aacagcctgc gcctgggtgt agccgacttc acaggcaaag agcccgtcgg gtttgagcac
     2221 tcgatccaga tctgccagga tctgagcgta cccctccgtt cccgcttcac ctccgacaag
     2281 tgccatcttc ggttcaaaat cacggacttc acgctgtaag tcagcatatt ccgcctgtga
     2341 aatgtatgga gggttggtga ccaccacatc aactcgttca gcaggtggga gatcccgcac
     2401 ggcgcttaaa taattgcttt ccaggaattg aatccggtct gccacacaat gagtcaccgc
     2461 attctgccgg gcaatcgcaa gtgcatctgg cgaaagatca agcgcgagga cctgagcggc
     2521 tggcagtttc accgccagcg tcacggccag gcagccggag ccagtcccaa tatcaatgat
     2581 gcgtgggcag gtcaggtttt ggtgacggca aaactcaagc gccacttcaa ccagaaattc
     2641 agtttccggg cgtgggatca aaacggcggg tgatacctta aactccagcc cataaaactc
     2701 ttgtaaccca ataagatatt gcgctggctc accctgagca cgccgggaaa aagcatctcg
     2761 caatctggtg gcatccacag cggatacggt gtattccgga tgggcaatca gtgtagtgtg
     2821 atccagtttc aaaataccgc acaccagtag tgatgccgtt tggtgcggcg cagggatttt
     2881 agcggcacgc aacagcgccg tgccttcacg aagaagtatt ttgagtgtgg gagattccga
     2941 catggaaaca atctcaagtg accagataaa aaaagaacga ggggctttat accggtctaa
     3001 agcccctcgt tgcaaactgt ttgagtttag ggttcagggt tcagggttca gggttcaggg
     3061 ttcagggttc agggttcag
//
LOCUS       DAOQDO010000531         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25026_length_3078_cov_2.552431, whole genome shotgun sequence.
ACCESSION   DAOQDO010000531 DAOQDO010000000
VERSION     DAOQDO010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25026_length_3078_cov_2.552431"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..3020
                     /locus_tag="PKZ53_09610"
     CDS             129..3020
                     /locus_tag="PKZ53_09610"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNJ40733.1"
                     /translation="MSSQIIFRYLSGVFFALTLCFASAVPAMALTMPPPAPTVSISPT
                     SDTLPFKGTRQFTATVTGTPMTTVIWSVNDIPGGNSSVGTISTSGLYTAPAALPPANP
                     VMVKAKSAADQTVSAVATVTVRYPTPVVQWFSPAKVPLGQSIFTLNGSQFYNGAVVML
                     DGTPLPTTYVSATQLKATSNVMQSSSGYITVANPGPAGATSTPRLIEFGQGITVTITP
                     STAMVAPAAQQQFTANVSGTTNTTVYWYVTGGSANGTITSSGLYKAPNSAPASPVTIR
                     AVCAVNSERSATATVTVTNGAPQTISVSISPTSASVQINAQQQFTASVTGTANTAVTW
                     QVNGVTGGNATTGTITDGGRYTAPATVPAGAVTVTAVSQADSTKKANATVTVTNQPQA
                     ISISISPTSASVQTGTTRQFTATVTGSANTAVTWQVNGVTGGNATTGTITASGLYTAP
                     GAIPAGTVTVRAVSVADPTKNASAGVNFVDPQAISVGRFLEQATFGPTPQLTAYVKQV
                     GIQQYLDEQFNAPESVYPNGIASSQGEMTDQFLWHMLTGQDQLRQRVKYALSEVIVIS
                     RNKNYYPNMLIPWQQILSKHAFGNYKNLLKEMTLDASMGNFLDMVNSTKPGVAGGANE
                     NYPRELLQLFSIGLYQLNPDGSQKLDGNGKPIPTYTQTDIRQLALALTGWTYATAGGT
                     PQSPNPNYYPGPMVPLPAYHDTSSKTFLGQTLPANQTMQKDLDDVIEIVFNHPNVGPF
                     IVTRLIRYLVTSNPTPGYVARMSAVFNDNGQGVRGDMKAVVRAILLDPEARNDNPGND
                     FGRLRTPLQHHIALLRVLGGTLSQPSQIAYAYTNMGESILDAPSVFGHYSPGFRVPKQ
                     SPPLFGPEFQIYGPGELVNRANLLWNWMNYYQTGIWDLQWLFTLGSDHVACVNAVDNL
                     LLYGRMSPGLRQQLLTALQSSQAAGADAKMRALTVLYLTAMSSEYQVAH"
BASE COUNT          723 a          916 c          798 g          641 t
ORIGIN      
        1 tggtcgttgt ttttccgcat caccttattg agcagacaag actgaagctt gacgcctggt
       61 tcaccagtag ctttatccaa aagcggtgtc aggccgccgc actccaaaaa cggcacagga
      121 gaaataacgt gagttcacaa attatatttc gatatctgtc aggtgtgttc ttcgcgctga
      181 cgttgtgttt tgcctcagcg gttccagcga tggcactgac gatgccccca ccggcaccaa
      241 ccgtcagtat tagcccgaca agcgataccc ttccattcaa agggacccgg caatttaccg
      301 caactgttac gggcacgccc atgacgacgg tcatctggtc ggtcaacgat attccaggcg
      361 gcaattcgtc ggttggcacc attagcacca gcggcctgta cacggcccct gccgccctgc
      421 cgccggccaa tccggttatg gtgaaagcga aaagtgcggc tgatcagaca gtgtcagcgg
      481 tcgcaaccgt cacggtgcgc tatccgaccc cggtggtaca gtggttttca ccagccaaag
      541 ttcctctcgg ccagtctatt ttcaccctca atggaagcca gttttacaac ggcgccgtgg
      601 tgatgctcga tggaacaccg ttaccgacca cctatgtttc cgcaacccaa ttgaaggcaa
      661 ccagtaacgt catgcaatcc agcagcggat acattacggt tgcaaatcct gggcctgcgg
      721 gggcgacgtc aacaccgcgt ctgatcgaat ttggacaggg catcactgtt acaattacac
      781 catcaacagc catggtggca cctgccgccc agcagcaatt tacggccaat gtctcaggga
      841 cgaccaacac caccgtttac tggtatgtca ctggtggcag tgccaatgga accatcacct
      901 ccagtgggtt gtacaaggct ccgaattcag ccccggccag cccggtgacg attcgggcgg
      961 tgtgtgctgt caatagcgag cggtcagcaa cggccaccgt gacggtcacc aacggagcgc
     1021 cgcaaaccat ttcagtttca atctcaccaa cgtcagcttc agtccaaatc aatgcccagc
     1081 agcaatttac cgcctcggtg accggcacgg ccaacacggc agtgacatgg caggtcaacg
     1141 gtgtcacagg tggcaacgca acgactggca ccatcacgga tggcgggcgc tacaccgccc
     1201 cagccacagt tcccgccggg gcagtcacgg tcacagccgt gagtcaggcc gattcaacca
     1261 aaaaggccaa tgccacggtg acagtcacca atcagccaca ggccatttcg atttcgatct
     1321 ctccgacttc ggcttcagtg caaactggca ccacacgaca atttacggct acggtcactg
     1381 gttcggccaa caccgccgtc acgtggcagg tcaatggtgt cactggcggc aatgcgacaa
     1441 ctggcacgat taccgccagc gggttgtaca cggcgccagg ggcaattccg gcggggactg
     1501 tgaccgtcag agccgtgagc gtggctgatc cgacgaaaaa tgccagtgcc ggcgtgaact
     1561 tcgttgatcc acaggcgata tcggtcggtc gtttcctgga acaggccacg tttggaccga
     1621 caccgcaact gacggcctat gtcaaacagg ttggcattca gcagtacctg gacgaacaat
     1681 tcaacgctcc tgagtcggtc tatccgaacg gcattgcttc ttctcaagga gaaatgaccg
     1741 atcagttcct gtggcacatg ctgaccggtc aggatcagct tcgccagcga gtgaaatatg
     1801 ccttgagcga agtcatcgtc atttcgcgca acaagaacta ttaccccaat atgctgattc
     1861 cctggcagca aatcctgagc aaacatgcct ttggcaacta caaaaatctg ctcaaggaaa
     1921 tgacgctcga tgcctcgatg gggaatttcc tggatatggt caacagcacc aaaccgggtg
     1981 tggccggcgg cgccaacgaa aactatccac gtgaattgct ccagctcttc agtatcggcc
     2041 tgtaccagct caacccggat gggtcacaga aactcgatgg aaacggcaag ccaattccaa
     2101 cctataccca gactgacatc cggcaactgg cgctggccct caccggatgg acctatgcca
     2161 cggcaggcgg cacaccacaa tcaccaaacc cgaattatta ccccgggccg atggtgccgt
     2221 taccggccta tcacgacacc tcgtcaaaga cctttctggg gcagacactc ccggccaatc
     2281 agaccatgca gaaagatctc gatgatgtga tcgaaattgt tttcaatcac ccaaatgtcg
     2341 ggccgtttat tgtcacccgg ctgattcgct acctggtgac cagcaatcca acgcccggtt
     2401 atgtggcacg aatgtccgcg gttttcaatg acaacggaca gggtgtacgg ggcgatatga
     2461 aagctgtcgt ccgggcaatc ctgcttgacc cagaagcccg caacgacaat ccaggtaatg
     2521 atttcgggcg gctgcgaaca ccacttcagc accacattgc cttgctgcgt gtgctgggcg
     2581 gcaccctttc ccaaccatcg caaattgcct atgcctacac caatatgggc gagagcattc
     2641 tggatgcacc atcagttttt ggccattact caccgggctt ccgcgttccg aaacaatctc
     2701 caccgctttt tggaccggaa tttcagattt acggccctgg tgaactggtc aatcgggcca
     2761 atctgctctg gaactggatg aattattacc agaccggaat ttgggacttg cagtggctct
     2821 tcacactcgg cagcgatcac gtggcctgtg tcaatgcggt tgataatctg ttgctctatg
     2881 gacggatgtc acctgggttg cgccaacaat tgctgaccgc cttgcaaagc tcccaggcgg
     2941 ctggcgccga tgccaagatg cgtgccttaa ccgtgctcta tctgaccgcg atgtcgagtg
     3001 aatatcaggt ggcacattag ggctgaaaaa ccctcgggct cagggcttgg ggctgaagac
     3061 tcgcaagctc ggggcaat
//
LOCUS       DAOQDO010000532         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25027_length_3078_cov_2.465762, whole genome shotgun sequence.
ACCESSION   DAOQDO010000532 DAOQDO010000000
VERSION     DAOQDO010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25027_length_3078_cov_2.465762"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..653)
                     /locus_tag="PKZ53_09615"
     CDS             complement(78..653)
                     /locus_tag="PKZ53_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ40734.1"
                     /translation="MTHTTDPKAITELLLKATHGNREAADQLIDAVYDELRQRARYYL
                     NREFHPDHTLQPTALVHEAYLRLIKQDQVVWQNRTHFFAMASEMMRRILVDYARTQQA
                     EKRGGEAIRLTLDAALDLAVPQPDINLIVLDDALNELSELDPRQAKLVELRYFGGLSV
                     EETAEVLQLSVATVMREWKITKMWLYRKLGG"
     gene            1223..>3078
                     /locus_tag="PKZ53_09620"
     CDS             1223..>3078
                     /locus_tag="PKZ53_09620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ40735.1"
                     /translation="MTPEHWQVIKGIIEQALDYEPAQRNSFLQQVCAHDPSLLTEVKQ
                     FLTLDSKLDQFLESFALVNLPPSTLVPADHLPTLYPEPVASFAPNDTFLGKYLVLRVL
                     GQGGMGTVYLARHLTLGTLFAIKVLKPHLIQSRDAIERFQREARITAHIEHPNVVRVF
                     DVGVEENQCYLVMEYLNGESLRQRLNRHKQFSVAQIAMWIDQVYAALQIVHQHGVIHR
                     DLKPDNIFFHRQGDQEVVKILDFGIAKPASFAETMTDPGSICGTPAYMSPEQCTGDKL
                     DEASDLYSLGVILFEMLTGTRPFESEKPLTVMYRHVNLPPPDVRHLVPTIPDAVARLI
                     QALLSKSPQKRIAVAHTLVATFQAAAQGHSLLDTTPHPVVDTHPNVTDSSRVTLPLQA
                     AFRQPRRLQKWMYGGLAGVILVALGIGVQTWKPGLSSSTAPTVVRHLPVAQPVPTLKK
                     DLGDRFVFISGGEVSIGANPGDCQKLPGCRIEPDELPAHRVELSPFYLCKFEVTNQEY
                     QEFIMATGHPSPPHWPKGTFPLESEDLPITNVSWLDATAYCSWCSTRDKIPFRLPTEA
                     EWEHAARGPNRTIFTWGDRWQSQWANTNPAPNTYPPLLSILQPPNNTTDCSA"
BASE COUNT          692 a          788 c          788 g          810 t
ORIGIN      
        1 atttcttcag cccattttct tcagcccgat ttcttcagcc cgatttcttc agcccgattt
       61 cttcagccct ggttttttca ccccccgagc ttgcgataca gccacatttt ggtgattttc
      121 cattcgcgca tcaccgtggc gactgaaagc tgcaggactt cagccgtttc ttcgacggag
      181 aggccgccga aatagcggag ttcgaccagt ttggcctgac gtggatcaag ttcgctcagt
      241 tcattgaggg catcatcgag cacaatcagg ttaatgtcag gttggggaac tgccagatcc
      301 agggcggcat ccagtgtgag ccggatggct tcgcctccgc gtttttccgc ctgctgggtg
      361 cgggcataat caaccagaat gcgccgcatc atctcagacg ccatggcaaa aaaatgagtt
      421 cggttttgcc agacaacctg atcttgtttg atcagccgga gataggcttc gtggacaagt
      481 gctgttggct gcagggtatg gtctggatga aattcacgat tcagatagta tctggcacgt
      541 tgacggagtt cgtcatacac ggcatcaatt aattggtcgg ctgcttcgcg attgccgtgg
      601 gttgctttga gcaacaattc tgtaattgct ttggggtcag tggtgtgggt catgtgagtt
      661 gtattcggtc aggttcaaaa aatcaggctg aggctggaaa attcaagtgt ttgatcgtgg
      721 atgaatcaca gggattcact cagcataggg atgttggacg tgagtagttg agcccaaaac
      781 gctgaaaaat agtggattgc gaaagtgaag gcaaggttca agttcattcc tggatcccgg
      841 atggtttcaa ccagtctcaa tttttttatt ctggaaataa cttattcact ttactggttt
      901 aagggcttgt cagtaccacc tgcgtgagcg ggtggggttg gaaacaagca aacccaccca
      961 ctgatgcggg tggtactgac tcagtggttt tggctgattc gtctccaaca caaaatcggg
     1021 tagaaataga caaattgaga tttgagaaag ccaaaactgc tttgcgactc gtagcccttg
     1081 agcattcaca cctgaatgtt gtcttcactc caggacaatt tcaacttcaa gcggttcctc
     1141 tcttcacaat catctgactc aatctggaag ccgttcaacc ctttctgatt cggtgtcaat
     1201 ttcgatttca ggagaccagg ttttgacccc ggaacattgg caggtcatta aaggcattat
     1261 cgaacaggct ttggattatg agccggccca gcggaattcg tttctccagc aggtctgtgc
     1321 gcatgaccca tcacttttga ctgaagtgaa gcaattcctg acgctggatt caaagctgga
     1381 tcagtttctt gaatcatttg cactggtcaa tcttccgcct tcaacgctgg tcccagccga
     1441 tcatttgccg acgttatacc ctgaacccgt ggccagcttt gccccaaacg acacatttct
     1501 cggaaaatat ctggtcctgc gggttcttgg tcagggaggc atggggaccg tgtatctggc
     1561 ccggcatctc acccttggga ccttgtttgc cattaaggtg ttaaagcccc atttgattca
     1621 aagccgtgat gcgattgagc gatttcagcg ggaagcccgg attaccgccc acatcgagca
     1681 tccgaatgtg gtgcgggtct ttgacgtcgg cgtcgaagaa aaccagtgtt acctggtgat
     1741 ggaatatctc aatggtgagt cgctcagaca acggctgaac cggcacaaac agttttctgt
     1801 cgcccagatt gccatgtgga ttgatcaggt ctatgccgcg ctccagattg tccatcagca
     1861 tggggtcatt caccgcgatt taaagcctga caatattttc tttcaccgcc agggtgatca
     1921 ggaggttgtc aaaatcctcg attttggaat tgccaaaccg gcctcgtttg ctgaaaccat
     1981 gaccgatccc ggttcgattt gtgggactcc ggcctatatg tctccggaac agtgcaccgg
     2041 agataaactg gacgaagctt ctgacctgta ttcgcttggc gtgattttat ttgagatgct
     2101 gaccggtacc cggccatttg aatctgaaaa accactgacg gtgatgtatc ggcacgttaa
     2161 cctgccgccg ccggatgttc gccatctcgt tccgacaatt ccagacgctg tcgccaggct
     2221 catccaggca ctgctttcca agtcgcctca gaaacggatt gcggttgcgc acactctggt
     2281 ggcgacgttt caggctgcag cccaggggca cagtttgctg gataccacgc cgcacccggt
     2341 ggttgataca cacccaaatg tgactgattc cagccgggtg acgctgcctt tgcaggctgc
     2401 ctttcgccag cctcgccgtc ttcagaaatg gatgtatggt ggactggccg gggtgattct
     2461 tgtggctttg ggaattggcg ttcaaacctg gaaaccgggt ttgtcatcca gcacagcccc
     2521 gactgtggtt cgacatctgc cagtggctca acccgttcca actctgaaaa aagacctggg
     2581 tgaccggttt gtttttattt caggggggga agtctcgatt ggagccaacc ctggtgattg
     2641 tcagaagctt ccaggctgtc ggattgaacc ggatgaattg cccgcccatc gagttgaatt
     2701 atctccgttt tacctctgca agtttgaagt gaccaatcag gagtatcagg aatttataat
     2761 ggcgaccggc catccatctc cgccacactg gccaaaaggg acattccctt tggagagcga
     2821 agacctgccc attaccaatg tgagctggct ggatgcaact gcctattgtt cctggtgctc
     2881 aactcgtgac aagattccat ttcggcttcc caccgaagct gagtgggaac atgccgcccg
     2941 cggtcccaac cgtaccattt ttacctgggg tgaccgctgg caatcacagt gggccaacac
     3001 caatccagcc ccaaacacct atcctccact gttatcaatt ttacagccac caaataacac
     3061 aacggattgc agtgcttt
//
LOCUS       DAOQDO010000533         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25046_length_3076_cov_2.856670, whole genome shotgun sequence.
ACCESSION   DAOQDO010000533 DAOQDO010000000
VERSION     DAOQDO010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25046_length_3076_cov_2.856670"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKZ53_09625"
     CDS             <1..525
                     /locus_tag="PKZ53_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40736.1"
                     /translation="QYQLLNHPAPVVALPAPAIDVLEDRSEVSVRTVRLHIASRRNAS
                     LVKLVLKKENGLRSCKLNGVPLDLNFPAWSGSNRWATVWYWGFPESGAELTFDLAPGS
                     PLDFLVTDVTFDLPQLPDRNWVPRNPNVMSMPHGFGVAERTLITSSFQLNRLAKTGTN
                     PGLAARASSFYHTR"
     gene            682..1110
                     /locus_tag="PKZ53_09630"
     CDS             682..1110
                     /locus_tag="PKZ53_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40737.1"
                     /translation="MNQRPEPLLPGTTPDKLDYWCQTDLPLDVIFARFATGFITRDSE
                     MDAENVWCWAEGTTEDGQLSFNISRKHHAGHEPERTQLPIEPVSIRFIRHQPNLNDSE
                     LGQKLANALQLEIFLGTMHYMGGDEFTFLAQTSFQPAQHH"
     gene            1125..1919
                     /locus_tag="PKZ53_09635"
     CDS             1125..1919
                     /locus_tag="PKZ53_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ40738.1"
                     /translation="MNHKEAGRFWNENAEAWTKLVRAGYDLYRNYFNTPAFFAMLPDI
                     NGLTGLDIGCGEGYNTRLLAQQGAKITAVDISELFIDYAQQAEAESPLGITYLVGSAV
                     ELPFADQSFDFATGFMSLMDVPELEKALSEAYRILKPGGFFQFSISHPCFSTAHRRNL
                     RGADGLTYAIEVGNYFHNLDGEIEEWIFGAAPPAMKEGLSRFKVPRFTYTLAQWINTL
                     IATGFVMERLEEPYPDEETVRAYPYLQDAQVVAYFLHVRVRKPAGA"
     gene            complement(1969..>3076)
                     /locus_tag="PKZ53_09640"
     CDS             complement(1969..>3076)
                     /locus_tag="PKZ53_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNJ40739.1"
                     /translation="KKVIPPFTATFVGVTWLARNFTRQHMILIFCVLFGVGFLTFAWL
                     LANPGAPVIWSFYIFGDMFNTVMLMLFWAFLSDIVTTSEAKRLYGVIGLGGVCGGLFG
                     AFFVATTVTKLGRENILLMCVIPMALIAVIVWLVNQQVEKEHASTIGMPAHPECTAAS
                     EGIQILLESKYLLAIAGMVIFYELISSIIDFQLSATVETKVLGSVAKTAYFSQIAIAQ
                     GFVSVFAQLFVTSYVMRKFGVGTALLILPVAILMGSVGYLIFANLLFVTVLSVSDNSL
                     SYSVNQSAREILYVPTSRCTKYKAKAIIDMSIQRFAKVLAVLVNLAAAWYLSLENIRW
                     LSLACLTVLLLWVAVIRYADRNFQQLTAEAEATA"
BASE COUNT          812 a          809 c          761 g          694 t
ORIGIN      
        1 cagtatcagt tgctcaatca cccggcgccg gtcgtggcgt tgccagcgcc ggcaattgat
       61 gtgctggaag atcgcagtga ggtcagcgtc cggacggttc ggctccatat cgcatcccgg
      121 cgtaatgcca gcctggtcaa actggtactc aagaaagaaa acgggcttcg ctcctgcaag
      181 ctcaatggtg taccgctcga tttgaatttt ccagcctggt cgggttcaaa ccgatgggca
      241 acggtctggt actggggatt tccagaatct ggtgccgaac tgacctttga cttagcaccg
      301 ggcagcccgc ttgacttcct ggtgaccgat gtcacgtttg atctgcccca actgccggac
      361 cggaactggg tgccacgcaa tccaaatgtc atgtcaatgc cgcatgggtt cggagtggct
      421 gaacgaacac tgatcaccag ttcttttcaa ttgaatcggc tggccaaaac tggaaccaat
      481 ccagggctgg cggccagggc ttccagtttt taccacacgc gatagccgtc accatcacga
      541 gttttgaggc gagccgcttt caaggtgcgg ctcgccgttt ctttccatat ccagatatgc
      601 ggcctgtgag ttcttttttt ctttgcaaaa tatgtttaag ctctgcccct ggaattgggg
      661 atgctctaca cgaggaaaag aatgaaccag cgacctgaac ctctcttacc aggcaccact
      721 ccagacaagc tggattactg gtgccagact gaccttccac tcgatgtcat ttttgctcga
      781 tttgcgaccg ggtttatcac ccgtgacagt gagatggatg ctgagaacgt gtggtgttgg
      841 gccgaaggaa caaccgaaga tggccaactt tcatttaaca tcagtcgaaa acaccatgcc
      901 ggccatgagc cagaaagaac acaactccca atcgaaccgg tttcgatcag attcatacgg
      961 caccaaccaa acctgaatga ctcagagctg ggacaaaaat tggccaacgc cctccaattg
     1021 gaaatttttc ttgggaccat gcactatatg ggcggcgacg agtttacgtt tctcgcccaa
     1081 acatcttttc aacccgctca acaccattga acacgaaaaa atttatgaat cacaaagaag
     1141 ccggacgctt ttggaatgaa aatgctgaag cctggaccaa actggttcgg gcaggctatg
     1201 acctctatcg gaattatttt aacaccccag cgttttttgc catgttgccg gatataaatg
     1261 ggcttacggg cctcgatatt ggctgcggtg aaggctacaa cacccggctc ctggcacaac
     1321 aaggggcaaa aatcacggcg gttgacatct cggaactctt tattgactat gcgcagcaag
     1381 ccgaagccga gtcgccgctg ggcattacct atctggtcgg cagcgccgtc gagttaccct
     1441 ttgccgatca atcatttgac tttgccaccg ggtttatgag cctgatggat gttccagaac
     1501 tggaaaaagc gctgtccgaa gcctatcgaa ttttaaaacc aggtgggttc tttcagtttt
     1561 ccatctcgca tccgtgcttt agtaccgcac accggagaaa tctgcgtggg gcggacggcc
     1621 tgacctatgc catcgaagtc ggaaactatt tccacaatct ggatggtgaa attgaagagt
     1681 ggatttttgg ggcggccccg cctgccatga aagaaggact ctccaggttt aaagtcccca
     1741 gatttaccta cacgctggca cagtggatca atactctgat tgcaaccggg tttgtgatgg
     1801 aacgactcga agaaccttac ccggatgagg aaaccgtccg ggcgtaccct tacctgcagg
     1861 atgcccaggt ggtggcctat tttctccatg tcagagttcg aaaacccgct ggggcatagg
     1921 tcatggaaaa agcagccgaa accagatgga gcgttttctt ccttggggtc aggccgtggc
     1981 ttcggcttcg gcagtcaatt gctgaaaatt gcggtcagcg taccgaatca cggccaccca
     2041 caacagcaaa accgtcagac aggccagtga cagccagcgg atgttttcga gacttaaata
     2101 ccaggcggcg gccaggttca ccaggacagc cagaaccttg gcaaagcgct ggattgacat
     2161 atcaatgatg gccttggctt tatatttggt gcatcgactg gtgggaacat acaaaatttc
     2221 ccgggctgac tggttgactg aataactcag gctgttgtca gaaactgata acaccgtgac
     2281 aaacaacaag ttcgcaaaaa tcaaataccc aaccgacccc atcaggatcg caactggtaa
     2341 aatcaggagc gctgtcccga cgccaaattt ccgcatgaca tagctggtga caaacaactg
     2401 cgcaaacacc gaaacaaatc cctgggcaat ggcaatctgg ctgaaatagg cagttttggc
     2461 aaccgatcct aaaacttttg tttcaacggt ggcgctcaat tgaaaatcaa taatgctgga
     2521 aatgagttcg taaaaaataa ccattccggc aatggcgagc agatactttg attcgagcag
     2581 aatttgaatg ccttcactgg cagccgtgca ctctgggtgg gccggcatgc caatggtgga
     2641 ggcatgttct ttttccacct gctggttcac aagccagaca atcaccgcaa tcaatgccat
     2701 ggggatcaca cacatcaaca agatgttttc acggccaagc tttgtgaccg ttgtggccac
     2761 aaaaaatgcc ccgaacaatc cgccacacac gccgcccaat ccaatgacac catacaaccg
     2821 tttggcttcg ctggtggtaa caatatcact caggaaagcc caaaatagca ttaacatcac
     2881 ggtgttaaac atgtccccga aaatataaaa cgaccagata accggagcac ctggattggc
     2941 gagcaaccac gcaaaggtga gaaacccgac gccaaacaac acgcaaaaga tcaaaatcat
     3001 gtgttgccgg gtaaaatttc gtgccagcca ggtgacgccc acgaacgttg cggtgaacgg
     3061 cgggatcact ttttta
//
LOCUS       DAOQDO010000534         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25251_length_3062_cov_3.569338, whole genome shotgun sequence.
ACCESSION   DAOQDO010000534 DAOQDO010000000
VERSION     DAOQDO010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25251_length_3062_cov_3.569338"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKZ53_09645"
     CDS             complement(<1..275)
                     /locus_tag="PKZ53_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40740.1"
                     /translation="MRPMEETELGVNLIFFPEESLVDQHTRHEIESVSRLSAELVDLY
                     LQVKSPIPLLRLQDETDASLTRHRLLAAGVRTAMIPDADLTLKQLPHR"
     gene            complement(570..2069)
                     /locus_tag="PKZ53_09650"
     CDS             complement(570..2069)
                     /locus_tag="PKZ53_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ40741.1"
                     /translation="MPNQAVNLPEPGVRSRWILVTVFSLSLTVGVVVGMLVMAVLGLP
                     HSRSLRQVWAAAWSPDQPAGSLSFSIARVAENVEPSVVHITTVDNPEGDLVGRGTGSG
                     FIVDKEGYILTNYHVVRDATRINVKLFNGIESPARLIAVDEETDIAVVKIASRTPLQP
                     VKFGDSDALKVGEWVVAIGSPFGLDQTVTAGIISAKERVTDKRRNLQQFIQTDAAINP
                     GNSGGPLLNLEGEVVGINTQIATQDGRFNGISFAIPSVTAKDVYRQLVTTGQVVRGYV
                     GVFLDQVSPQFAQVYNIPRGQGALVQYVEENGPAARSGLKSGDVIVAFDGHGVRDDRD
                     LVRWVASTPVGNEVKIEFYRDGRSQTTQLRVDERVMPSTKFPSVPPLLKRNYSRSIER
                     PAPREFGVQVGVLTRLKVQQMGLAGNLNESDPGVVVTGVEPLSVAADAKIRERDLIIA
                     INRKPISSEQDFYEVVKKLGPNDDVVVVVKRALPGERRAVVNFLSFTMP"
     gene            2140..>3062
                     /locus_tag="PKZ53_09655"
     CDS             2140..>3062
                     /locus_tag="PKZ53_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ40742.1"
                     /translation="MLHLASSRALIATPGLGGAHLQLIDYFVRKAGPMHLHRVNQVKQ
                     NHPERKKNRSRWVIVGVGILAALLLAQTACTPPPPSSPTTADSVPKKGPLADGKFRVG
                     FISTQTSTSVDVWNDQGNQALAIMEKEFGATIIKRDAVLTPKERERAFTALAEEGCNV
                     IIGHGREFMPEALLIAGSYPNTLFVVTNGDQEASNVASLVFELGQASYLAGAIAAKIS
                     KTGHVGAIGRKDNPDSRKILTAFRNGALKANPDIHFSLAYLDDPQDIAKAREATLALI
                     DRGSDLVAHDCGAAAPGVFEAVKERKVLVFGA"
BASE COUNT          754 a          848 c          764 g          696 t
ORIGIN      
        1 cggtggggca actgtttgag cgtgagatcg gcatcaggaa tcattgccgt ccgcacacca
       61 gccgccagca gtcggtggcg agtcaggctg gcgtcagttt catcctgcag cctcaaaagt
      121 ggtatgggtg atttgacttg caaatataaa tcaaccagtt cggctgatag ccggcttacg
      181 ctttcgattt cgtgccgcgt atgctgatca accaggcttt cttcaggaaa aaaaatcaga
      241 ttgaccccaa gttcggtttc ctccattggt cgcacaacag gcagcttgtg aaactcagag
      301 ccttcaataa ctggcagttg cccaccacag taaatgcata agagacgctg ggaattgtta
      361 tttcggtgac agagggggca ggccacaagg gtgacttcaa ccacgggaaa tacctcgctt
      421 tttgaatttt tgcagagttg aggtgttgag atttagatct gaggctgtac atggctgaag
      481 gagtgagcac tgaaggatga cataaaacca atttttcaac tctgagtttt cttcagccca
      541 cactcttaac ccggcgtctt cagccccact tacggcatgg tgaacgaaag gaaatttacc
      601 acagctcgcc gctcaccagg gagggctcgt ttcacaacca ccacaacatc atcgttggga
      661 cccagttttt tcacaacctc atagaaatcc tgttcagagg aaatcggctt gcggttgatg
      721 gcaatgatca gatcgcgttc gcgaatcttt gcatcagccg ccacactcaa tggttcaaca
      781 cccgtcacaa ccacaccagg gtctgattca ttgagatttc ccgccagtcc catttgctga
      841 actttcaacc gggtcaatac gccaacctgg acgccgaact cacgtggggc cgggcgctca
      901 atcgaacggc tatagttacg tttgagcaac ggaggcaccg acgggaattt ggttgacggc
      961 attacccgtt catcaacccg taactgggtc gtctgactgc gaccatcgcg ataaaattca
     1021 attttcacct cgttcccaac cggcgttgag gcaacccagc gaaccagatc acggtcatcc
     1081 cgtaccccat ggccgtcaaa cgcaacgatt acatctccgc tttttaagcc ggagcgggcc
     1141 gccggcccgt tttcctcaac gtactgaacc agggcgccct gtccacgagg aatgttatac
     1201 acctgggcaa attgcggtga gacctgatcc aggaataccc ccacatagcc gcgaacaacc
     1261 tgtcccgtcg taaccagttg gcgataaaca tcttttgcgg tcactgaagg aatggcaaaa
     1321 ctaatgccgt taaatcggcc atcctgggtc gcaatctggg tgttgatccc caccacttct
     1381 ccctccagat tcagtaatgg gccacctgaa ttcccaggat tgatcgcggc atcagtctgg
     1441 ataaattgtt gtaaattgcg ccgcttgtcg gtgactcgct ctttggcact gataatcccg
     1501 gcagtcactg tctgatccaa accaaaggga cttccaatcg caacaaccca ttcaccgacc
     1561 ttcaaggcat cggaatcacc aaatttgacg ggttgcaggg gtgtgcggga tgcaatcttg
     1621 accaccgcaa tgtcagtttc ttcatccaca gcaatcaggc gggcaggcga ctcgatacca
     1681 ttaaataatt tgacattaat ccgcgtggca tcgcggacaa cgtgataatt cgtcaggatg
     1741 tacccttctt tatcaacgat aaacccggaa ccggtaccac gcccaaccag atcgccttca
     1801 ggattatcca ccgttgtaat atgcaccact gaaggctcaa cattttcagc cacccgggca
     1861 atcgagaagg acagggagcc ggctggttga tcaggtgacc aggctgcggc ccatacctgc
     1921 cggagcgagc gactgtgagg caggccgaga accgccatca cgagcatgcc cacaacaact
     1981 ccgacagtca ggctcaggga aaaaacagtc accagaatcc aacgagaccg cacaccgggc
     2041 tctggtaggt taacagcttg gttcggcatg gttagacttt cctttttcaa agtagtgcag
     2101 ttatattttc cgggaagttg cgccggctcg caatagtgct tgctccatct ggcttcatca
     2161 cgtgccctga tcgcgacacc gggtctgggc ggtgcccatt tgcaactgat tgattatttc
     2221 gttcgaaagg caggccccat gcaccttcac cgggtcaacc aggtcaaaca aaaccatcca
     2281 gagcgaaaaa aaaaccggtc acgctgggtc attgtgggag ttggaatcct ggcagccctg
     2341 ctactggccc aaactgcctg tacccctccc cctccctctt caccaaccac agcagattca
     2401 gttccgaaaa aaggcccgct cgctgatgga aaattccggg ttgggttcat ttcgacgcaa
     2461 acctcaacgt cggttgatgt gtggaatgat cagggaaacc aggcactggc gattatggag
     2521 aaggaatttg gggccaccat tatcaaacga gatgcggtgt tgacccccaa agagcgggaa
     2581 cgggcattta cagccctggc tgaagaaggc tgcaatgtga tcattggcca tggccgcgag
     2641 tttatgccgg aagccttatt aattgctggt tcttatccca atacgttgtt tgtggtcacc
     2701 aatggtgacc aggaagcaag caatgtcgcc agtctcgtgt ttgaacttgg acaggcatca
     2761 tatctggctg gtgcgattgc agccaaaatt tccaaaaccg gccatgttgg ggcaatcggt
     2821 cgcaaggaca atccggactc tcggaaaatt ctgacagctt ttcgcaatgg cgccctcaaa
     2881 gccaatccag acatccattt ctccctggcc tatctggatg accctcaaga catcgccaaa
     2941 gcccgtgaag ccaccctggc tctgattgac cggggttctg accttgtggc tcacgattgt
     3001 ggcgcggcgg cccccggtgt gtttgaagcg gtcaaagaac ggaaagtact cgtctttggc
     3061 gc
//
LOCUS       DAOQDO010000535         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25256_length_3062_cov_2.802793, whole genome shotgun sequence.
ACCESSION   DAOQDO010000535 DAOQDO010000000
VERSION     DAOQDO010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25256_length_3062_cov_2.802793"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /gene="sufD"
                     /locus_tag="PKZ53_09660"
     CDS             <1..1077
                     /gene="sufD"
                     /locus_tag="PKZ53_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNJ40743.1"
                     /translation="AMARSPMGTTFLNHELSAFPVLPAGCVVGLLADVCQTHPEAVQK
                     YFGQVATPDQNGFTALNTALMGDAVVIYIPRGKVVETPINLFFVSDHAADFQATFPRV
                     LIVAEQASIATVVENYLALDDESGRFGERPYLTNVVTEIVLEDGAILEHYKVQKESLN
                     AYHIANTRARIQQAAVLNGYSFALGGQISRNQIDVAFTAPGGECALQGLYYVSGKQLA
                     DVHLSVDHAHPNCTSQILYKGMLDDKARGVFDGKVWVRTDAQGTNANQTNKNLLLSTS
                     ARVDTKPQLEIFADDVKCSHGATVGQLNKDEIFYLLSRGLPLEQARTMLTFAFAQEVV
                     DRIKIESIKSGLGRAISTRIAGRG"
     gene            1208..2467
                     /locus_tag="PKZ53_09665"
     CDS             1208..2467
                     /locus_tag="PKZ53_09665"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNJ40744.1"
                     /translation="MAVAQNSLAVAEPPTFNVEHIRADFPMLRQQVNGKPLVYLDNAA
                     TSQKPHVVIDRISRYYSEEYATVRRSIHKLSEKATIAYEHTREVAARFLNAPSTRQII
                     YTRGTTEAINLVARGYGAKFISAGDEVLISATEHHANIVPWQMICAEKGATLRVIPVN
                     DQGELILEEYEKLLTDRTRIVCVGHVSNALGTIHPIKHMIELAHAKNIPFLVDGAQAA
                     PHMKVDVQDLDCDFYAFSGHKAVGPTGIGILYGKADLLEKMNPFLGGGDMIESVTFEK
                     TTYAPIPHKFEAGTPAIAEVIGLGTAIDYLTGIGLDRIQAYEHELLEYGTQVLSAIPQ
                     VKLIGTAAHKAAIISFILDGIHPHDIGHFLDQEGVAIRAGHHCAQPVMKRFGVPATAR
                     ASFSFYNTREEIDELAKAILKTIEVFS"
     gene            2468..2920
                     /locus_tag="PKZ53_09670"
     CDS             2468..2920
                     /locus_tag="PKZ53_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNJ40745.1"
                     /translation="MSSYNNELYQQVILDHNKNPRNFKALENPTCMCEGHNPLCGDQI
                     TVFINLDGPVIKDITFTGVGCAICKASASMMTAFIKGKPKDEAELLFHEFHKMVKGEL
                     NVNSEAHHLGRLAIFSNVSHFPVRVKCASLPWHAMHSALEQKGSASTE"
BASE COUNT          788 a          755 c          795 g          724 t
ORIGIN      
        1 gcgatggcgc gctcgcccat gggaacgacc ttcctcaatc acgaactctc cgcgttccca
       61 gttctcccag caggatgtgt tgtgggtctg ctggctgatg tctgtcaaac gcatccggaa
      121 gcagtccaaa aatactttgg gcaggtcgcc actccagatc agaatgggtt tactgcgctc
      181 aataccgccc tgatgggtga tgcggtggtg atttatattc cgcgaggcaa agtggttgag
      241 acaccaatca atttgttttt cgtctcagat cacgcggctg attttcaggc gacgttccca
      301 cgggtgctca tcgttgctga gcaggccagc atcgcgacgg tggttgaaaa ctacctggcg
      361 ctcgatgatg aatcaggccg gtttggcgaa cgtccttacc tgaccaatgt cgtcactgaa
      421 attgtgcttg aagacggtgc catcctggag cactacaaag tccagaaaga aagcctgaac
      481 gcctaccata ttgccaatac cagggctcga atccagcagg cggcggtgct caacggctat
      541 tcttttgcgc tcggcggtca gatttcccgc aaccagattg atgtcgcgtt tacggctccc
      601 ggcggcgaat gtgcattgca ggggttatat tacgtgagcg ggaaacaact ggcggatgtg
      661 catttatcgg ttgaccatgc tcatccgaac tgcaccagcc agattctcta caaaggaatg
      721 ctggatgata aggcacgtgg cgtgtttgat gggaaagtat gggtccgaac cgacgcccag
      781 ggaacaaacg ccaaccagac caacaagaat ttgctgcttt caaccagtgc ccgtgttgac
      841 accaaaccac agcttgaaat ttttgctgac gacgtcaaat gcagccacgg cgctaccgtt
      901 ggtcagctca acaaagacga aattttctat ttactgagtc gcgggcttcc actggaacag
      961 gcccgaacca tgttgacttt tgcttttgct caggaagttg tagatcggat caagattgaa
     1021 tccatcaaat caggactggg ccgggccatt tcaacccgca ttgccggacg cgggtaagaa
     1081 ccctgaccga agagtgaaga atgaagaatg aagcattgag aatgaagaac agcgcacatg
     1141 ctttcttctc aatcaaagaa caaattcata attcatactt cgagaagact tttggagact
     1201 aagtgatatg gcagttgcac aaaattcttt ggcggtggct gaaccgccca cgtttaatgt
     1261 cgaacacatt cgggctgatt ttccaatgct cagacagcag gtgaatggga aaccgctggt
     1321 ctatctggac aatgccgcca cgagccagaa accgcacgtc gtaattgacc gcatcagccg
     1381 atattattcg gaagaatatg cgaccgtgcg gcgcagcatc cataaacttt ctgaaaaagc
     1441 caccattgcc tatgaacaca cccgcgaagt ggccgcccgg tttttaaacg ctccatcaac
     1501 ccggcagatt atctacacac gtggcacaac cgaagcgatc aatctggtgg cccgtggcta
     1561 tggcgcaaaa tttatttcag ccggagacga agttctgatt tcagccaccg aacaccacgc
     1621 caacattgtg ccctggcaaa tgatttgtgc tgaaaagggg gcgacgctaa gggtgatccc
     1681 ggtcaatgac cagggtgaat tgatcctgga agaatatgaa aaactgctca ctgaccgcac
     1741 ccgaattgta tgtgtcgggc acgtttccaa cgcgcttggg accattcacc cgatcaagca
     1801 catgatcgaa ctggcccacg ccaaaaatat cccattcctg gtggatggcg ctcaggccgc
     1861 accacatatg aaggttgacg ttcaggacct ggactgcgac ttttatgcgt tttcaggcca
     1921 taaagcggtt ggtccgacag ggattgggat tttgtatggc aaggctgacc tgcttgaaaa
     1981 gatgaatcca tttctgggcg gtggggatat gatcgaatcg gtcacgtttg aaaaaacgac
     2041 ctatgctccg attccacata agtttgaagc tggcacccct gcgattgccg aagtcatcgg
     2101 tctggggaca gccattgatt acctgactgg aattggcctg gatcgcattc aggcgtatga
     2161 acacgaattg ttggaatacg gcacccaggt gctgtcggca attccacagg tgaagcttat
     2221 tgggacagca gcccacaaag cggcgattat ttcctttatt ttagatggca ttcatccaca
     2281 cgatattggc catttccttg atcaagaagg agttgccatt cgtgctggtc accattgcgc
     2341 ccagccggtg atgaaacggt ttggcgttcc cgccacggct cgggcgtcgt tttctttcta
     2401 caatacccga gaggaaattg atgaactggc caaagcgatt ctgaaaacga tagaggtatt
     2461 tagctaaatg tcgagctaca acaacgagtt gtaccagcag gtcattctcg atcacaacaa
     2521 gaatcccagg aattttaagg cgcttgaaaa tccaacgtgc atgtgcgaag ggcacaaccc
     2581 gctctgtggt gatcagataa ccgtctttat caacctggat ggaccggtga tcaaggacat
     2641 tactttcact ggtgtgggat gtgcgatttg caaagcttca gcctcgatga tgacggcttt
     2701 tattaaggga aaacccaaag acgaggccga attactgttt cacgaatttc acaaaatggt
     2761 caaaggcgag ctgaatgtga attccgaagc gcatcatctg ggccggctgg cgattttttc
     2821 aaacgtcagt cattttccgg tgcgggtcaa atgtgccagt ctcccgtggc atgcgatgca
     2881 ctcggcttta gagcaaaaag gatctgcctc aaccgaatga ggcacaggag cctggcttgc
     2941 agaggccggc tctgacaacg gtgttcgtct cgtacaagca agtgggctgt atggaatgat
     3001 tgggtacatt ccatacagcc tttttttgaa ataaaaccag tgtgagcagg gatggattca
     3061 at
//
LOCUS       DAOQDO010000536         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25257_length_3062_cov_2.746924, whole genome shotgun sequence.
ACCESSION   DAOQDO010000536 DAOQDO010000000
VERSION     DAOQDO010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25257_length_3062_cov_2.746924"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1021
                     /locus_tag="PKZ53_09675"
     CDS             29..1021
                     /locus_tag="PKZ53_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNJ40746.1"
                     /translation="MSTANTTTGQITAFLKHHFRHFNAASLIDAAEAYQRHLDQGGKM
                     MVTLAGAMSSAELGISLAEMIRQDKIHIISCTGANLEEDLFNLVAHDYYERVPHYRDL
                     TPQDELALLERHMNRVTDTCIPEMEAMRRLETAMVDEWTRADQAGERYFPHEFMYRVL
                     KSGALEQYYQIDTRDSWMMAAMEKNLPMVVPGWEDSTMGNMYAGHCITGDVKNVHTVR
                     TGIEYMIELADYYTNTSADHSIGFFQIGGGIAGDFPICVVPMLHQDLQRTGVPVWGYF
                     CQISDSTTSYGSYSGAVPNEKITWGKLGVDTPKYIIESDASIVAPLMFAMILGQ"
     gene            1262..>3062
                     /locus_tag="PKZ53_09680"
     CDS             1262..>3062
                     /locus_tag="PKZ53_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40747.1"
                     /translation="MTHQITSSQFLRRAMAVLVGLCFWVVGCGMVTPVLAGIAHPTRF
                     ERLTIEQGMPSNWVRALHQDTHGFLWVGTQDGLARYDGYHFTIFRANPGDSTSLSDNA
                     VWDLQEDQSGRLWVATSGGLNLFLPDQETFIQYQHQPHQATSLSHDVVNTLFVDRLGQ
                     LWVGTNDGLNRFDVASGQFIRFNLDEPGSDPAARNRITAITQAGPDSLWVGTGDGLLE
                     FNPDTSRFRSFRHDPARPGSLGAREVRSLLVDRSGQLWVGTLGGGLNLYHPETSTFEV
                     FLFSPNGEGNNVSCILEDSLGQIWITSYLRSIEGGLHRFHRATKQFESFAYDPKNPYS
                     LSWNFGTTLLEDASGLLWIGTSRGLNKYDLRAKSFTSFLLHPENPFDLGDNYYAVEID
                     SRGDLWLGTDSPVLHQVNLKTGDVKTFEGNGTVAGLGETTGFYSIVEDRRGLIWFGTA
                     TSGLLQYDPQKKSFRAFSHDPKNPNSISSNYVGRILEDPSGDLWVGTDRGLNRFDPQT
                     GRFTQVAAEFVLPGAAGPFSVSALWRDRQGQLWIGTGPTISTNAGGGAGLLRLNPQTG
                     AVVAFRHDTHDAASLSSDIITSIVEDRSGTLWVG"
BASE COUNT          676 a          777 c          854 g          755 t
ORIGIN      
        1 accgcatttt tgatcttgag agggtcctat gagcactgca aacaccacca ccggccagat
       61 tacggctttt ttgaaacacc attttcggca ttttaatgcg gccagcctga ttgacgccgc
      121 cgaagcctat caacgccacc tggatcaggg tggaaagatg atggtcacgc tggctggcgc
      181 gatgagttcg gctgaactcg gcatttcgct ggcggaaatg attcgccagg ataaaatcca
      241 catcatttcc tgcacggggg ccaatcttga agaagacctc tttaacctgg tggcacacga
      301 ttattatgag cgcgtgccgc actatcgcga cctcaccccg caggatgaac tggcgctcct
      361 cgaacgccac atgaaccgcg tcactgatac ctgcattccg gaaatggaag ccatgcgccg
      421 gctcgaaacc gccatggttg atgaatggac acgtgccgac caggccggag agcgctattt
      481 cccgcatgaa tttatgtatc gggttctgaa atcaggcgcc ctggaacagt attaccagat
      541 tgataccagg gattcctgga tgatggcagc gatggaaaag aatttgccaa tggtggttcc
      601 gggctgggaa gattcaacca tgggcaatat gtatgccggg cactgcatta cgggcgatgt
      661 gaaaaacgtc cacacggtgc gcaccgggat tgagtacatg attgaactgg cggactacta
      721 cacgaacact tcagccgatc attcaattgg attcttccag attggcggcg gcatcgcggg
      781 cgatttcccg atttgcgtgg tgccgatgct acaccaggat ttgcagcgca cgggtgtccc
      841 ggtgtggggc tatttctgcc agatcagtga ttcgacaacc agttatggct cctattcggg
      901 cgctgttccc aacgaaaaaa tcacctgggg gaaactcggg gttgatacgc cgaaatacat
      961 tatcgagtca gatgcctcga ttgtcgctcc gctgatgttt gccatgattt tggggcagta
     1021 gtttcagagt gaaggagcga aggcgcgaag ggtgaaaccg gcatcagcac gtgtaacccg
     1081 tggaaaaaag tgggtagcac agagtaagat agagcgattc tgaaaacacc acagaaactc
     1141 tctaggacca cttaaagcat tttttcctgg ctatttaacc tgttgtgaac gcgttcacaa
     1201 tttgcctttt acacctggtt ctgtttcgct ccttcgctct ttcacgactt cactcatctc
     1261 tatgacccac cagatcacca gttctcaatt tttgagacga gcgatggccg tgcttgttgg
     1321 cctgtgcttt tgggtggttg ggtgtgggat ggtgacacct gtcctggctg gcattgcaca
     1381 tccgacgcgg tttgagcggc tcaccatcga acaggggatg ccgtccaact gggtgcgggc
     1441 cttgcaccag gatacccacg ggtttttgtg ggtcggcacg caggatggtt tggcccgcta
     1501 tgatggatac cacttcacca tttttcgggc caatcccggt gattcgacgt cgctgtctga
     1561 caatgccgtt tgggatttgc aagaggacca gtcaggcagg ctgtgggttg ccaccagcgg
     1621 tggattgaac ctctttcttc cagatcaaga aaccttcatt cagtatcaac accagccaca
     1681 ccaggccacc agtttaagcc acgatgtggt caacaccttg tttgttgatc ggttgggcca
     1741 gctctgggtt ggtaccaatg acggattaaa tcggtttgat gtggccagcg gccagtttat
     1801 ccggttcaac ttggatgagc ctggaagcga cccggcagcc cgaaatcgaa taacggccat
     1861 tacacaggct ggtcctgact cgctctgggt cgggactggg gatggactcc tcgaatttaa
     1921 cccagacacg agccggtttc ggtcatttcg ccatgacccg gcacgccctg ggagtttagg
     1981 tgccagagag gttcgttcgc tcctggttga tcgttcgggg caactctggg ttgggacact
     2041 tggtggcggg ttaaatctct atcatcctga aacgagtacc tttgaagtct ttttgttttc
     2101 acccaacgga gaaggaaaca atgtctcctg cattctggaa gacagccttg ggcaaatctg
     2161 gatcacgtcc tatctgcgca gtattgaagg tggtctgcat cgtttccatc gagcgacaaa
     2221 acagtttgaa tcgtttgcct atgatccgaa aaatccgtac agcctgagct ggaattttgg
     2281 cacgacgcta ctggaagacg cctctggact gctctggatt ggcacgtcgc gtgggctcaa
     2341 caaatatgat ttgcgcgcca aaagctttac cagttttttg cttcacccgg aaaacccttt
     2401 tgatttgggt gacaattact atgccgtgga aattgattcg cgcggcgact tatggctggg
     2461 gacggatagt ccggtcctgc atcaggtcaa cctcaaaacc ggcgatgtca aaacatttga
     2521 ggggaatggg acggtggctg gacttgggga gacgaccggg ttttattcaa tcgtcgaaga
     2581 ccgacgtggc ctgatctggt ttgggacagc caccagtggc ttgttgcaat atgatcctca
     2641 aaagaagtct tttcgagcgt tctcgcacga tccaaaaaat ccaaattcca tcagttcaaa
     2701 ctatgttggt cgaattcttg aggacccgtc cggtgactta tgggttggga ctgaccgtgg
     2761 cttaaatcgg tttgacccac aaaccgggcg ctttacgcag gtggcggctg agtttgttct
     2821 gcctggggcg gcggggccat tttctgtttc cgccttatgg cgtgaccgtc aggggcagct
     2881 ttggattggt acggggccca ccatcagcac caatgcgggt ggcggtgccg ggttgctccg
     2941 gctcaaccct caaaccggcg ccgtggttgc ttttcggcac gatacccacg acgcggccag
     3001 cctatcgagc gatatcatca cttcgattgt cgaagaccga agcggtacac tctgggttgg
     3061 ga
//
LOCUS       DAOQDO010000537         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25344_length_3056_cov_3.679107, whole genome shotgun sequence.
ACCESSION   DAOQDO010000537 DAOQDO010000000
VERSION     DAOQDO010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25344_length_3056_cov_3.679107"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PKZ53_09685"
     CDS             <1..1883
                     /locus_tag="PKZ53_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ40748.1"
                     /translation="AGNLWLSTNTGLCKFHTVTHTCHNYDLRDGLQSNEFNTGTCFQN
                     AQGEMFFGGINGFNVFHPETILDNPFIPPVVITSFRKLNQQMTGADLQQVIELRYFEN
                     LIAFEFAALNYTLPEKNQYAYRLEGFDKNWILCGDRRYASYTNLEPGTYVFCVKGSNN
                     DGVWNETGIKVRLRVIPPPWKTWWAYSFYLILISLAVFGGYRFQVNRVQARVRLHEAR
                     LRAETAEAQAQSAQARAEAIQIEARAAARLAEQNAELDRKNKELERKNEELVASQQQA
                     DRIFSALAEALPGTVLDGKYRLDEKIGAGGFGAVFQATHLQLNRLVAVKVFKPKPGND
                     SADAIERFRQEGISTSRLNHINAIQVLDSGISSEGIAYLVMELLQGHTLADEIRTHRL
                     LPLHRCAEINAQICDALAEAHRLGIIHRDIKPENVFLNQTPVGEVVKVLDFGIAKMLS
                     EDTDEEMAHLTATGGLVGTPLYMAPERLERKPYDGRSDVYSAGIMVYEMLTGKTPFDP
                     GSKGLVGIVMAHLRQTPPPLRQFFVDLPAEIESIVMSALEKNPQSRPTAKEFGEHLSR
                     AVLNLASSPEITIVVRPGTTTENTNLPTVSLQFSTISDIPTGTPLVAPRDIRRSLGQE
                     QE"
     gene            complement(2151..>3056)
                     /locus_tag="PKZ53_09690"
     CDS             complement(2151..>3056)
                     /locus_tag="PKZ53_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ40749.1"
                     /translation="WETLGYGVGSAPGQFRGPEAVVSMEDFFWPKIICCSAPIYVADT
                     LNNRIQWSTDGGTNWEVFASVGSGLDQVRAPQGLAIDNEGNLIVADTGNGRVLRFEEG
                     IPGFAVLLASKGSGSGQVGAPFGLAMDYFEGDTLYVCDQLNSRIVRIDDVDDVNVRDT
                     GVIVASLGVGLNKVKNPQGICVDFDGTVYIADTGNNRVLRWINGNPANSTALALLGSQ
                     LGQVNRPEGVTINYGFFSQPWRKEIFTGPMLIVSDTGNNRIQGRVLDSPGQWDLIGYP
                     NGVGSLTGQFRFPSKIHYSGFFFFD"
BASE COUNT          788 a          823 c          740 g          705 t
ORIGIN      
        1 gggctggcaa tttgtggctc agcaccaata ccgggctgtg caaatttcac accgtcaccc
       61 acacctgcca caactatgac ctgcgggatg gattgcaaag caatgaattc aacaccggaa
      121 cctgctttca aaatgcccag ggagaaatgt tttttggcgg catcaacggg tttaatgtct
      181 ttcaccctga gacaatcctg gataacccat ttattccacc ggttgtgatt accagttttc
      241 gcaaactcaa tcagcagatg acgggcgctg acctccagca ggtgattgag ttgcgatatt
      301 ttgaaaatct gattgctttt gaatttgcag ccctcaacta cacactgccg gaaaaaaacc
      361 agtatgccta tcgcctcgaa gggtttgata agaactggat cctgtgtggt gatcggcgct
      421 atgccagcta taccaacctg gaaccgggca cctatgtctt ttgcgtgaaa ggttccaaca
      481 atgatggcgt gtggaatgaa accgggatca aggttcggct gcgggtaatc cctcctccct
      541 ggaaaacctg gtgggcctat agcttttatt tgatactgat cagtctggcg gtgtttggcg
      601 ggtatcgctt tcaagttaat cgggttcagg cccgagtccg gttgcacgaa gcccggttac
      661 gggccgaaac cgctgaagcc caggcccaaa gtgcccaggc cagagccgaa gccatacaga
      721 ttgaagcccg ggccgccgct cgactggctg agcaaaatgc cgaactggac cgcaaaaaca
      781 aagaactcga acgaaaaaat gaagaactgg tcgcctctca gcagcaagcc gaccgtattt
      841 tttcagccct ggccgaggca ttgcccggta cggtcctgga cggaaaatat cggctggatg
      901 aaaaaatcgg cgctgggggg tttggcgctg tatttcaagc gacccatctc caactcaacc
      961 ggctggtggc cgtcaaagtg tttaaaccca aaccgggcaa tgatagtgcc gatgccatcg
     1021 agcgctttcg acaggaagga atttccacct cacgcctcaa tcatatcaat gccattcagg
     1081 tcctggattc agggatttca agcgaaggaa ttgcttatct cgtgatggaa ttgctccagg
     1141 gtcacacact ggcagatgaa atccgaactc atcgcctgtt gcctttgcac cgatgcgctg
     1201 aaatcaatgc ccagatttgt gacgccctgg ccgaagcaca ccgattggga attatccatc
     1261 gcgacatcaa accagaaaat gtcttcctca accagacacc agtcggtgaa gtggtcaaag
     1321 tcctggattt tggaattgct aaaatgttga gcgaagacac cgatgaagaa atggcacacc
     1381 ttaccgcaac gggtgggctg gtcgggacgc cgctctatat ggcccctgaa cggctggaac
     1441 gaaagcccta tgatggccgg tctgatgtct atagcgcggg cattatggtg tatgaaatgt
     1501 tgactggaaa gactccgttt gaccctggtt ccaaaggact tgtcggcatt gtgatggctc
     1561 acctgcggca aactccgcca cccttgagac agttttttgt ggatcttccg gctgaaattg
     1621 aatcaattgt gatgtcagcc ctggagaaaa atccacaaag ccggccaacc gccaaagaat
     1681 ttggcgaaca cttatccagg gccgtactca acctggcatc ttcacctgag atcacgattg
     1741 tggtccgccc tggcaccaca actgaaaaca ccaatctacc gaccgtcagc cttcagtttt
     1801 cgacaatttc cgacatccca accggaacac cgctcgtggc accacgtgat atccggcggt
     1861 cattgggtca ggagcaggag tagtaccatt ttaggctgag ggtacggggc taagggctga
     1921 gggaaataca attttttcaa taatttagcc ttcccatcat acgggaaata tagagggaaa
     1981 aggtggtgtg ctctggccgg gtatggtttt tgttttcagt aacttgccac tggtttgtct
     2041 ggcccgaaag accgcgcctc aaaacaatac ggggaaaaac gacgattgag ggtcaatcgt
     2101 cgtctttccc cgtcagtttg agcactcacc ttcaagtgag atttctttgg ttaatcaaag
     2161 aaaaagaagc cggagtagtg aatcttgctt gggaaccgga attgaccggt cagggagcca
     2221 actccatttg gatacccaat caaatcccac tgaccgggtg aatccagcac ccgtccctga
     2281 atccgattat tgcccgtatc actcacaatc aacattgggc cggtgaaaat ttcctttctc
     2341 cagggttgtg aaaagaagcc gtaattgatg gttacgcctt cagggcgatt cacctgtcca
     2401 agctgtgatc cgagcaaggc cagcgccgtt gagttcgctg gatttccatt gatccaccgt
     2461 aaaacccgat tgttgccggt gtcggcaatg tagacggttc cgtcaaaatc aacacagatt
     2521 ccctgtggat tcttcacttt gtttaaacca accccaagtg aagcaacaat aacccctgtg
     2581 tcccgaacat tgacatcgtc aacatcatca attcgcacaa tccgactgtt taactggtcg
     2641 cagacataga gggtatcccc ttcaaaataa tccatcgcca atccaaacgg cgccccgacc
     2701 tggccactgc cgctgccttt tgaagccagc agcaccgcaa atccgggaat gccctcttca
     2761 aaccgcaaaa ctcgcccatt cccggtgtca gccacaatca ggttgccttc attatcaatc
     2821 gccaatcctt gtggcgcccg cacctgatcc agtccagagc caactgaagc aaacacctcc
     2881 cagttggttc cgccatcggt tgaccactga atccggttat tgagggtatc agccacataa
     2941 atcggagctg aacaacaaat aatttttggc cagaaaaagt cctccatgct gaccacagct
     3001 tccggacctc ggaattgccc tggcgctgaa ccaacgccat agcccaatgt ttccca
//
LOCUS       DAOQDO010000538         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25416_length_3051_cov_3.571095, whole genome shotgun sequence.
ACCESSION   DAOQDO010000538 DAOQDO010000000
VERSION     DAOQDO010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25416_length_3051_cov_3.571095"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PKZ53_09695"
     CDS             <1..1853
                     /locus_tag="PKZ53_09695"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ40750.1"
                     /translation="EEETFLLVRIQPPATLKPGSKIELAAAADWLVCKEICLPGHADL
                     KLGLTVADGVAKPSTWSEAFSATRAKLPLEGADWKLQAAIQNDKILLKATQPADFKDQ
                     LKQIVFLPQEGLIIEDDAPQPVKRVENGYLIELKRSMIDPSKVPSKLEGILISEGWRG
                     EKSERSLRVNVEIGPMAALGNLAAVTETAKPGVAGTKPGAPASFLTILIAIGSAFVGG
                     LILNLMPCVLPVLSIKVLSFVQQAGEDKSKVWKHGAVFTLGVLTLFWGLAGSLLILRA
                     GGEQIGWGFQLQSPGFLIFICSLLFLLGLNLFGVFEIVLNVGGNLSSQSGYSGSFFSG
                     ALATLVATPCTAPFMGSALGFAIAQPPIVSMLIFTSLGLGMAFPYLMLSLFPALMRFV
                     PRPGVWMEAFKQFMGFLMMATVVWLAWVFGLQVGVNGLIALMGGFVVMGIGAWVLGRW
                     GNLMMASRTRLIAHVVAGVLIAGGIGLVWTNSRDLAPSASASTQSTTTAEGITWEAFT
                     PERFAEVRATGKPVFIDFTAAWCLSCQVNEKVALSKPEIAAEFKKRGIVTMKADWTSR
                     NEAITRKLAEFGRNGVPLYVLYTKDQNQPPMVLPEVITPSIVMDALQKVN"
     gene            1899..2498
                     /locus_tag="PKZ53_09700"
     CDS             1899..2498
                     /locus_tag="PKZ53_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ40751.1"
                     /translation="MTTKMKNLALLAILGVFLVIGCSPVSGAGDVAVGSAAPNFTGTD
                     SNGKNHSLTDFKGKFVVLEWFNPDCPFVKAQYDPKHMQELQKLFTKKGVVWLSVDSSA
                     PGKQGHLTGDQANAVLKEKGASPTALLLDPEGKIGRLYGAKTTPHMFVIDPSGKLIYA
                     GAIDDKQGKNYVKAALEEALAGKPVSVAATQPYGCSVKY"
     gene            2789..>3051
                     /locus_tag="PKZ53_09705"
     CDS             2789..>3051
                     /locus_tag="PKZ53_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ40752.1"
                     /translation="MIATARKTTRRRVGTSSRRARRQSPVQHTLTGNFRMHTAFPSRF
                     LPQTRNILVYLPPDYETNLTRHYPVFYMHDGQNLFDAATAYIPG"
BASE COUNT          702 a          750 c          807 g          792 t
ORIGIN      
        1 atgaagaaga aaccttcctc ctggtccgca tccaaccccc tgctacctta aaaccgggtt
       61 ccaaaatcga actggccgcc gccgccgact ggctggtgtg caaggaaatc tgcctgcccg
      121 gtcatgctga tttaaaactg ggcctgacgg tcgcggatgg ggttgccaaa ccatcaacct
      181 ggtctgaagc tttttctgcc acccgggcca aactcccgct tgaaggcgct gactggaaat
      241 tacaagccgc aattcaaaac gacaaaatcc tgctgaaagc aacccagccg gctgatttca
      301 aagatcagct caaacagatt gtgtttctgc ctcaagaagg gctgatcatt gaagacgatg
      361 ctcctcaacc cgtcaaacga gtcgaaaacg gctatctgat tgaactcaaa cggtcaatga
      421 ttgatccgtc aaaggtgccg tctaaactcg aaggaatttt gatttcagag ggctggcgtg
      481 gtgaaaagtc agaacgttca ttgcgagtca atgttgaaat tggaccgatg gctgcgcttg
      541 ggaatctggc tgccgtcact gagaccgcca aacccggtgt ggctggaacc aaacctggcg
      601 caccggcttc gtttctgacc attttgattg ccattggatc ggcctttgtc ggcgggttga
      661 ttctcaattt gatgccctgt gtgctgccgg tcctttcgat caaagtgttg agttttgtcc
      721 agcaggctgg tgaagacaaa agcaaagtct ggaaacacgg cgccgtgttt acgctcggtg
      781 tcctgaccct gttttggggg ctggccgggt cgttgctcat cctgcgggct ggcggagagc
      841 agattggctg ggggtttcag cttcagtcac cgggattttt gattttcatt tgttcattgc
      901 tgtttttact tggattgaat ctgtttggtg tctttgaaat tgtgctcaac gttggcggca
      961 acctgtcgtc gcagtccggg tattcgggat catttttcag cggtgcactg gctacgctgg
     1021 tggcaacccc atgcacggca ccatttatgg gatcagcgct tggttttgcc attgcccagc
     1081 caccgattgt ttccatgttg attttcacgt ctctggggct ggggatggcc tttccgtatt
     1141 tgatgctttc gctgttcccg gccttgatgc gatttgtgcc acggcccggt gtctggatgg
     1201 aagcgttcaa acagtttatg ggttttttga tgatggccac ggtggtctgg ctggcgtggg
     1261 tgtttgggct ccaggttggt gtcaatggct tgattgccct gatgggcggc tttgtggtga
     1321 tgggaattgg cgcctgggtt ctgggacgct ggggtaacct gatgatggct tcacggaccc
     1381 ggttgattgc ccacgttgtg gcgggtgtct tgatcgctgg gggcattgga ctggtgtgga
     1441 ccaattccag agacctggcg ccgtcagcat cggccagcac tcagtccacc acgacggctg
     1501 aaggcattac ctgggaggct tttaccccag aacgttttgc cgaagttcgc gctactggca
     1561 agccggtgtt tattgatttt acagcagcct ggtgtttgag ttgtcaggtc aatgaaaaag
     1621 tcgccctgag caaacctgaa attgcggctg agtttaaaaa gcgcggtatt gtgacgatga
     1681 aagctgactg gacatcgcgc aatgaagcca ttacccgcaa acttgccgag tttggacgca
     1741 acggtgtacc gctctatgtg ctctatacca aagatcagaa tcaacccccg atggttctac
     1801 ccgaagtgat tactcccagc atcgtgatgg atgccctgca aaaagtgaat taaatccaca
     1861 actcaaatct gtgaacctta gtttttagga gataaacaat gacaacaaag atgaagaacc
     1921 ttgcattact cgccatttta ggtgtgtttt tggtgattgg ctgcagcccg gtgagtggcg
     1981 ccggtgatgt ggcggttggt agcgctgccc caaatttcac cggtactgat agcaatggaa
     2041 aaaaccactc attaaccgat ttcaaaggta aatttgtggt tttagagtgg tttaacccgg
     2101 attgcccatt tgtaaaagcc caatatgatc caaagcacat gcaggaatta caaaagctct
     2161 tcaccaaaaa aggtgtggtc tggctttcgg ttgattcctc agcgcctggg aaacaaggcc
     2221 atctgaccgg agaccaggcc aatgccgtgc tcaaggaaaa aggtgcttcg ccaacggcat
     2281 tgctgcttga cccagaaggc aaaatcggac gtctctatgg tgccaaaacc acgccgcata
     2341 tgtttgtgat tgacccgagc gggaaactca tctacgccgg cgcaattgat gacaaacagg
     2401 gcaagaatta tgttaaagca gcactcgaag aagccctggc tggcaaaccc gtaagtgttg
     2461 ccgccaccca gccctatggc tgctcagtaa agtactaaaa tcattggggg ttcaggggtg
     2521 cggggaaact atcaggtttt gaatcgcttc gccttgaaat ttcaatttct ttcccacagg
     2581 tcgcgccagt cgcggccttt tccattcaaa cgggatagag ggcaggcgaa agcttgaact
     2641 caccagttct ttcatcacat ccatccttcg ttcccgtttg gtaccaatgt gaaatcggta
     2701 tttcgaaaag tgaataagct agatgattga ttgataatgc tttgtcttat tcattattca
     2761 tcattggtgt ttccaaggag aatttgcatt gattgccaca gcaagaaaaa caaccagacg
     2821 ccgcgttgga acttcctcac ggcgcgcacg gcgacagtca ccagttcaac atacgctgac
     2881 cgggaatttc cgaatgcaca cggcctttcc ctccaggttt ttaccgcaga ccagaaatat
     2941 cctggtctat ctgccgcctg actatgaaac caatctgacc cggcactacc cggtttttta
     3001 catgcacgac gggcagaatc tgtttgatgc cgccaccgct tacattcctg g
//
LOCUS       DAOQDO010000539         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25432_length_3050_cov_3.198664, whole genome shotgun sequence.
ACCESSION   DAOQDO010000539 DAOQDO010000000
VERSION     DAOQDO010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25432_length_3050_cov_3.198664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKZ53_09710"
     CDS             complement(<1..676)
                     /locus_tag="PKZ53_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ40753.1"
                     /translation="MTRQANLYGAVNLAQGMPDFEAPQELKDAACQAIQDGFNQYAIT
                     WGAPVLRKAIAEKAKQFNGIDCLPDENITVCCGATEAMMAAMLALVNPGDEVIAFQPF
                     YENYGPDGLLSGAKPVWVPMYAPDWKFDPEELRRAFGPKTKAIIINTPNNPTGRVFTR
                     EELTLIAELCQEHDVYALTDEIYEHIIYNDQPHISIATLPGMAERTVTISGLSKTYSV
                     TGWRLGY"
     gene            complement(718..1986)
                     /locus_tag="PKZ53_09715"
     CDS             complement(718..1986)
                     /locus_tag="PKZ53_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40754.1"
                     /translation="MLKDVKKATPWIFQRLLDRLENETDLETYEYDELETYPELEPLL
                     IQIATLPPQEQIAACWHFLKIGNDQFSEFPLGTVQFEQAIGSYVQAVQNARSSTQPLD
                     HYLDHLLEACEWDMCAFGSVLPKLKAGIDTLTTSEAGYRYLIGRLADSNIPDALDWMA
                     EYYLKLGEEEEFLNIRESHLETEAHFLQLADYWQSKRNTRRYVEVLERFVTELPVRIQ
                     IAQYAFAAEVEAPETSPVLLRLAAHYTARADNHNLCRILMTTAQYSQFTLELYQHIET
                     LATQLGIWPDQRRILHELARGNAQLLAEILLYEHRWDEVIAFANQDIAERAQILIAEK
                     IKDQFPLKAIGIYQKLVTDYLELGEDQHLERAWQHLSAIRSIFLSQTKDEPGWNAYVT
                     HLKETYAYHIELHEVLNQLVTSPLEKEFLC"
     gene            complement(2288..>3050)
                     /locus_tag="PKZ53_09720"
     CDS             complement(2288..>3050)
                     /locus_tag="PKZ53_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNJ40755.1"
                     /translation="TPMILTEHQGKVLTITLNNPAKSNSLHPDLVKSLLVVLKEAATN
                     QEVNAIVLTGAGKNFCAGLDLALLLTWTPDEKLAYLETVSSLFIQIWEQPQPVIAAVR
                     GAALAGGFDLAAFCDLRIASETAQFGQAEINIGLTQIIHPLYKSIGLARAKELSLTGL
                     PISAAEAYRIGLVNHVYPEVQVVSQSLELAQLMASKSRYALFETKRLTRELIDLDTKA
                     ALAEINRTFIGCLDSAEHKADLQRVYSKISKSKSS"
BASE COUNT          678 a          772 c          824 g          776 t
ORIGIN      
        1 agtacccgag ccgccagccc gttacgctgt aggtttttga cagtccgctg atggtcaccg
       61 ttcgttcggc cattcctggc agggttgcaa tgctgatgtg cggctgatcg ttgtagatga
      121 tgtgttcata aatttcatct gtcagcgcat agacatcgtg ttcctggcac agttcggcaa
      181 tcagggtcaa ttcttcgcgg gtgaaaacac gcccggttgg gttgtttggc gtgttgatga
      241 tgatggcttt ggtttttggc ccaaacgccc ggcggagttc ctctggatca aatttccagt
      301 ccggggcata catcggcacc cacaccggct tggcacctga aagcaagcca tccgggccgt
      361 agttttcata aaatggctga aacgcaatca cttcgtcacc agggtttacc agcgcgagca
      421 tggcggccat catcgcttcg gtggcgccac agcaaacggt gatgttttca tccggcaggc
      481 aatcaattcc attgaattgt ttggcttttt cagcaattgc tttgcgcagg accggtgcgc
      541 cccaggtaat cgcgtactgg ttgaagccgt cctgaattgc ctggcaggcc gcatctttta
      601 attcctgggg ggcttcgaaa tcaggcattc cttgagcgag attaacggcg ccgtacaaat
      661 tggcctgacg ggtcatttca cggatgaccg actcggtaaa aaacttgaca cgttcagcta
      721 acacaaaaac tccttttcca aaggggaagt caccagttga ttcaaaactt cgtgcaactc
      781 gatgtggtag gcataggttt ctttcagatg tgtgacatag gcgttccagc caggttcatc
      841 ctttgtctga gaaagaaaaa tgctgcggat agctgacagg tgctgccagg ctcgttccag
      901 gtgctgatct tcaccgagtt cgaggtaatc agtgaccagt ttttggtaaa tcccaattgc
      961 tttaagcggg aactggtctt tgatcttttc cgcaatcaag atctgggctc gttcggcaat
     1021 atcctggttg gcgaaggcaa tgacttcatc ccagcggtgt tcatagagca ggatttcagc
     1081 caggagttgc gcattgcctc gggccagttc gtgcaggatt cgccgctgat ccggccagat
     1141 tcccagttgg gtggccagtg tttcgatgtg ctggtacaac tcaagcgtaa actggctata
     1201 ttgagcggtg gtcatcaaaa tccggcagag attgtggttg tcagccctcg ccgtatagtg
     1261 cgctgccagt cgaagcaaca ccggactggt ctctggtgcc tcaacctcag cggcaaacgc
     1321 atattgagca atctggatcc gaaccggcaa ctcagtgaca aaccgctcca ggacttcaac
     1381 atatcgccgg gtatttctct ttgactgcca gtaatcggcc agttgcagaa agtgggcttc
     1441 ggtttccagg tgagactcgc ggatgttgag aaattcctct tcttcaccaa gcttcaagta
     1501 atactcagcc atccagtcaa gcgcgtccgg gatgttgctg tctgccaatc ttccaatcaa
     1561 ataacggtac ccggcttcag aagtggtcag tgtatcaatc ccggctttca gttttggcag
     1621 cacggaccca aacgcgcaca tgtcccattc acaggcttcc agcagatggt ccaggtagtg
     1681 atccagaggt tgagtgctgg accgggcgtt ttgaactgcc tgcacataac tgccaattgc
     1741 ctgctcaaac tggaccgttc ccagtggaaa ttcactaaac tggtcatttc cgattttgag
     1801 aaagtgccag caggctgcaa tttgttcttg tggcggtaag gtggcaattt ggattaagag
     1861 aggctcaagt tccggatagg tctccagttc gtcatattca tacgtctcaa ggtccgtttc
     1921 gttttccagc cgatcaagca gccgttgaaa aatccacggg gtcgcttttt ttacatcttt
     1981 gagcatggaa tgtagtactt ggtggttggt accatttacg tgcgtttgca tcccggaggg
     2041 atgaagcaag gttagccggt ggtcagcgtg gctttgcacg cgccaccacc ggatccaatc
     2101 agccccgatg tttttcccgc ttggcccgcg ccccacgggg cgcgggccgg gtgcggaaga
     2161 ggagatcttc aacttgtttt ttccggtggt agctcgcaaa gccttgcaac caaccggcta
     2221 agcttgctcc atcctttcag gatgagatcc atcggtcagg aaatgattcc tgttcagggt
     2281 tcaagcgtta gcttgacttc gatttgctga tttttgaata gacccgttgg aggtccgcct
     2341 tgtgttcggc tgagtcaagg caaccgataa acgtccgatt gatttcggcc agggcggctt
     2401 tggtgtcgag gtcaatcagt tcgcgagtaa ggcgcttggt ctcaaacagg gcatagcgtg
     2461 atttggaagc catcaactga gccagttcca gagattgaga cacaacctga acttctgggt
     2521 agacatgatt gaccaggcca atccggtacg cttctgcggc tgaaatcgga aggccggtca
     2581 gcgaaagctc tttggcccga gccagcccaa tggatttata aagcgggtga atgatctgcg
     2641 tcaggccaat attgatttcc gcttgaccaa actgagccgt ttcagaggca atgcgcaggt
     2701 cacaaaaggc agccagatca aatccgccag ccagggccgc accgcgcacg gcggcaatca
     2761 ctggctgtgg ttgctcccaa atttgaataa agagcgatga aaccgtctcc agataggcca
     2821 gtttttcgtc cggagtccag gtcaacagca atgccagatc cagcccggca cagaagtttt
     2881 tccccgcacc agtcaggaca atggcattta cctcctgatt ggttgccgcc tctttcaaaa
     2941 caaccagcag tgatttgacc aggtccggat gcagtgaatt actcttcgcc gggttattca
     3001 gggtgatggt gaggactttg ccttgatgtt cggtgaggat cataggagtg
//
LOCUS       DAOQDO010000540         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25539_length_3043_cov_3.187082, whole genome shotgun sequence.
ACCESSION   DAOQDO010000540 DAOQDO010000000
VERSION     DAOQDO010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25539_length_3043_cov_3.187082"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..2153
                     /gene="sppA"
                     /locus_tag="PKZ53_09725"
     CDS             336..2153
                     /gene="sppA"
                     /locus_tag="PKZ53_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNJ40756.1"
                     /translation="MRDFLKQVFASLTGLVLFSLLALVVVIFLIGSIISSTGGKPEPK
                     VDDKAVLVFNLSTNISDAAPDADPIKAFQDAAFGSRTKSISLSTVLDTLDKAAKDKRI
                     VGLYLYGNLQAEGYGSGFAALKEVREALERFRAAGKTITFYDMGIGEQEYYLASVANT
                     IALNPFGAVEMNGFASQPMFLADAFKKYGIGVQVTRVGKYKSAVEPYLLNKMSPENRE
                     QLENLLGDNWNEFLAAVGKSRNIPVADLQKIADEQALLIGEQAKQQKLVDKVVYFDEI
                     VTDLKKLTNTPDKDTTFRQISLAAYAKVSRESLGIAKESKNLIAVVYAEGEIVDGTGT
                     SDQIGGDRLAKQLRDLRQDPDVKAVVLRVNSPGGSASASEVIQREIILTKKAKPLIVS
                     MGSYAASGGYWISTYADHIFAEPNTITGSIGVFGLLLNVGQLANDHGVTWDTVKTGKY
                     ADLGTISRPKTDEELALIQKTVDQIYDQFLAKVSESRKLPREKVAEIAQGRVWSGIDG
                     KELGLVDELGGLEDAIKFAAKKANLGDNWKTTEYPKPTTTEEKIRELLTSDEQPDRLA
                     KTSPAVREFGRLQQELSILNSMNDPMHVYARLPFSIRID"
     gene            2295..>3043
                     /locus_tag="PKZ53_09730"
     CDS             2295..>3043
                     /locus_tag="PKZ53_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ40757.1"
                     /translation="MNEAELLTAKILIVDDIQANVLLFEAMLKSEGYCNVYGTTDSRK
                     AADLYRQYNPDLVLLDLDMPYLNGFDVMDQLKEIESESYLPVLVLTGLSDQSVRIRAL
                     EFGARDFLCKPFEHVEALTRIRNLVEVRLLHNAVREQNRILEEKVRERTRKLRETQLD
                     IIRRLGRAAEYRDNETGFHVIRMSHFSACLARTVGFSDTDCEILLYASPMHDVGKIGI
                     PDRILLKNAPLTSEEWTIMKTHTVIGSDLLAG"
BASE COUNT          794 a          775 c          751 g          723 t
ORIGIN      
        1 atttttttcc gtgttttccg tggtttccgt ggttaaaatg tctggaaatt ttcggcaaag
       61 tacttaccac ctcaaattga ttgaccactc actcctcgct cttaaaaagg tcaggcacag
      121 gtacaggttt ccaggaaagg tcaaccgaaa ccgcagttct gaacgtatgg ggaaatcccg
      181 tcgcatacaa aaatgccaaa cattacccac gttcacttgt ttttgggaga ctctggttga
      241 cccgttgaca acaaccgtgt aacccatgac acgaatcaaa gcgaggtttc tctcgcttct
      301 atcactgacc ttttggaaaa tgaggaagtt tcaacatgcg tgattttcta aagcaggtat
      361 ttgcgagtct caccgggctg gtgctcttta gtctgctggc cctggtggtc gtgattttct
      421 tgatcgggag tatcatcagc agcacaggcg gcaaacccga gccgaaagtg gatgacaaag
      481 cagttctggt ctttaatctt tcgaccaata tttcagatgc cgcaccagat gccgatccaa
      541 tcaaagcatt ccaggatgca gcctttggga gccgaaccaa aagtatttca ttgagcacgg
      601 tgctcgacac gttggataaa gcagctaaag acaagcgcat tgttggactc tatctctacg
      661 gcaacttaca agccgaagga tacggttctg gatttgccgc gctcaaggaa gttcgtgaag
      721 cactcgaacg attccgcgcc gccgggaaaa ctattacctt ctatgacatg ggaattggcg
      781 agcaggaata ctacctggcc tcagttgcca ataccatcgc cctcaacccg tttggcgccg
      841 tcgaaatgaa tgggtttgcc tcacagccga tgtttctggc cgacgctttc aaaaagtatg
      901 gaatcggcgt tcaggtcacc cgggttggaa aatacaaatc agccgttgag ccttaccttt
      961 taaataaaat gagtccggaa aatcgcgagc aactcgaaaa tttacttggc gataactgga
     1021 acgaattcct ggcggcggtt gggaaaagcc gtaatattcc agtggcggat cttcagaaaa
     1081 ttgccgatga acaggcgctc ttgatcggtg aacaggccaa acaacaaaaa ctggttgata
     1141 aagtggtgta ttttgacgaa atcgtcaccg acctcaagaa actcaccaac acgcctgaca
     1201 aagacaccac cttccgccaa atcagcctcg cggcatatgc caaagtctca cgcgagtctc
     1261 tgggcattgc caaagagtcc aagaatctga ttgccgtggt gtatgccgaa ggtgaaattg
     1321 ttgacgggac aggcacttca gaccagatcg gaggcgaccg gctggccaaa caactccgtg
     1381 atttacgcca ggatccagat gtcaaagccg tcgtgctgcg ggtcaacagc cccggtggta
     1441 gcgccagcgc ctcagaagtt atccagcggg aaatcatcct cactaaaaaa gccaagccat
     1501 tgattgtatc catgggatcg tatgccgctt ctggcggata ctggatttcg acctatgccg
     1561 atcacatctt tgctgaaccc aataccatta ccggttcgat tggcgtgttt gggttgttgc
     1621 tcaacgttgg ccaactggcc aatgaccatg gcgtgacctg ggataccgtc aaaacgggga
     1681 aatatgccga tttaggcacg atctcacgcc ccaaaaccga tgaagaactg gcgctcatcc
     1741 agaaaacggt tgatcaaatt tatgatcagt tcctggccaa agtcagcgaa tcgcggaaac
     1801 ttccccgcga aaaagtggct gaaattgccc agggtcgggt ctggtctggg attgatggca
     1861 aggaactggg tctggtggat gaacttggcg ggcttgagga tgcaatcaag tttgccgcga
     1921 agaaagccaa tctgggtgac aactggaaaa caactgaata tccaaaacca accacaaccg
     1981 aagaaaaaat ccgtgagttg ctgacctctg atgaacaacc tgaccggctg gccaaaacca
     2041 gcccggcggt acgtgaattt ggccgactgc aacaagaact ttcgattctc aactcaatga
     2101 acgacccgat gcacgtctat gcgcggttgc cattttcgat tcggattgac taactacgag
     2161 tgcttggtgc ttagtactta gtgcttagga atcaacgctt tccaagttcc aagcaccaag
     2221 tcgcaagcac caagccctaa gtactaagta ccagtcacct ctcaacttca cttcccgtct
     2281 cttcttaaac tccaatgaac gaagctgagc ttctaaccgc caaaatcttg attgtggatg
     2341 atattcaggc caatgtgttg ctgtttgaag ccatgttgaa gtctgaaggc tactgcaatg
     2401 tctacggcac gaccgattct cggaaggcgg ctgatttgta ccgccagtac aaccccgacc
     2461 tggtcctgct ggacctggac atgccgtacc tgaacgggtt tgatgtcatg gaccagttga
     2521 aagaaattga atctgagtcc tatcttccgg tcctggtttt gactggcctg tctgatcaga
     2581 gtgttcggat tcgggcgctt gagtttggcg cccgtgattt tttgtgcaaa ccctttgaac
     2641 acgtcgaagc cttaacccga atccgcaacc tggtcgaggt ccggctattg cacaacgccg
     2701 ttcgagagca aaatcggatt ttggaagaga aagtccgcga acgcactcgc aaactccgtg
     2761 aaacccagct tgatatcatt cgccggctcg ggcgggcggc tgaataccgg gacaatgaaa
     2821 ccggatttca tgtgattcgc atgagtcatt tcagtgcctg cctggctcgg acggtcggtt
     2881 ttagtgacac tgactgtgaa atcctgctct atgccagccc aatgcacgat gtcggcaaaa
     2941 ttgggatccc tgaccgcatc ctcttaaaaa atgccccgct gacgtccgaa gaatggacca
     3001 ttatgaagac ccacacggtc atcggttcgg acctcttagc cgg
//
LOCUS       DAOQDO010000541         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25571_length_3041_cov_3.700268, whole genome shotgun sequence.
ACCESSION   DAOQDO010000541 DAOQDO010000000
VERSION     DAOQDO010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25571_length_3041_cov_3.700268"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..2366)
                     /locus_tag="PKZ53_09735"
     CDS             complement(264..2366)
                     /locus_tag="PKZ53_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNJ40758.1"
                     /translation="MTAGNFIFIAVFLASISLFLFNSRKILEILKLGKSEPGNRLDNT
                     GERLKNMLSVAIAQQKILRDPLAGFLHLLIFWGFMVLTIGTIEAILEGFYSRFSYRLV
                     LGNDIFYVLTLLQEGFGACVLTAAVLLLARRYVSPPKRFSGVEMKPDSRMDATIVLLM
                     IITLMVSMFLANSFAPTEEKRITRDISGRTVSQQLAKVIEPKLSTSTTHILFEVSWWV
                     HIFTVFGFLNYLPFSKHLHVLTSVPNVFFTSLKSRGALKKMDFEAMEAEEEPVFGASD
                     IEHFTWKQLFDSLTCTECGRCTSVCPANLTGKPLSPRKIMMDIRDRLMEKGEVLIELK
                     KAGAHGAAENGNGDSKNGHSAKDILAEKLISEKFITPEELWSCTTCQACMQECPVNIE
                     HVPTILDMRRNLVMNEADFPGELQTLFTNLENKYSPWAFSHAARADWSEGMNIKTMAQ
                     AQNEDIEVLFWIGCAGSYDDRYRKVVQSVARLLQKANIKFAILGKEEKCTGDPARRAG
                     NEYLAQSLIQENVETMNGYGVKKVLTSCPHCFNTIKNEYPDFGGNYEVMHHSQYLSEL
                     VKSGRITLTQKIEATAVYHDSCYLGRSNDVYEAPRQTLVQIGGMKLVEMDRSRDKGMC
                     CGAGGARMFMEENVGTRVNVERAKQALETNPNVVASACPFCMTMLTDGVKAHENTQVK
                     VFDIAELLDQAAGGEKRV"
BASE COUNT          745 a          746 c          764 g          786 t
ORIGIN      
        1 caaaagaggc agccagcttt ggctacctct taaaaaatca gggctgaaga cttggggctg
       61 aagaaatcgg gctcagggct tggggctgaa aacttcgggc ctggggctga agacgtcggg
      121 ctgagggctg acgaattcgt tttatttcgt tcttttgcgc caggttcaat atccttagtt
      181 ccaagcccaa tgtcttccgc ccttagcccc aagcctgcga gtcttcagcc caacttcttc
      241 agcccaatgt cttccggcct gaactaaacc cgtttttccc cgccggctgc ctggtcgagc
      301 agttcggcaa tgtcaaacac tttgacctgg gtgttttcgt gggctttgac accatcggtg
      361 agcattgtca tacagaatgg acaggccgaa gcgaccacgt tcgggttggt ttcaagtgcc
      421 tgtttggctc gctcgacgtt gacgcgggtt ccgacatttt cttccataaa catccgggca
      481 ccgcccgcac cgcaacacat ccctttatca cgggagcggt ccatttcaac cagcttcatc
      541 cctccaattt gaaccagcgt ctggcgtggg gcttcataca catcgttgga gcggcccaaa
      601 tagcacgaat cgtgatagac ggcggtggct tcgatctttt gggtcaatgt gatccggcca
      661 gatttaacca gttcggaaag atactggctg tggtgcatga cttcatagtt gccgccaaag
      721 tccggatact cattcttgat agtgttaaag cagtgcggac aggacgtcag gacttttttc
      781 acgccgtacc cattcatggt ttcgacgttt tcctgaatca gcgattgggc cagatattcg
      841 tttccggcgc gacgggccgg gtcaccggta catttttctt ccttgcccag aatggcaaat
      901 ttgatattgg ctttttggag cagtcgggct acggattgga ccaccttgcg atagcggtcg
      961 tcataggacc cggcgcagcc aatccaaaac aagacctcga tatcttcgtt ctgagcctgg
     1021 gccatggttt tgatgttcat cccttcagac cagtcagcgc gagcggcgtg actaaacgcc
     1081 cagggagaat atttattttc aaggttggta aacaacgttt gcagttcccc tggaaagtcg
     1141 gcttcgttca tcaccaggtt ccggcgcata tcgagaatgg tcggaacgtg ctcgatgttg
     1201 accggacatt cctgcataca ggcctggcag gttgtacagg accagagttc ttcgggagtg
     1261 ataaattttt cgctgatgag tttttcagcc aggatatctt tggccgaatg tccatttttg
     1321 gaatcaccat tcccattttc agccgcccca tgagcgccag ctttcttgag ttcaatcagg
     1381 acttcgccct tttccatcaa ccggtcgcga atatccatca tgattttgcg tggggaaagc
     1441 ggcttgccgg tcagattggc tgggcagact gaggtacagc gtccgcattc ggtacaggtc
     1501 aggctgtcaa aaagctgttt ccaggtgaaa tgttcgatgt cagaagcacc aaagactggt
     1561 tcctcttcag cttccatggc ttcaaagtcc attttcttca acgcgccacg ggatttgagc
     1621 gaggtgaaga acacattggg cactgaggtc aggacgtgga ggtgtttgga gaagggaagg
     1681 tagttcaaaa agccaaaaac cgtgaaaata tgcacccacc acgacacctc aaacaggatg
     1741 tgggtggtgc tggttgacaa tttcggctca atcactttgg ccagttgttg tgaaacggtg
     1801 cggccagaga tatcgcgggt gatgcgtttt tcttctgttg gcgcaaacga gttggccaga
     1861 aacatcgaga ccatcaaggt aatgatcatc aggagcacga tggtggcatc catccggcta
     1921 tccggtttca tttcaacgcc actaaatcgt ttggggggac tgacatagcg tcgagcaagc
     1981 aacaaaacgg cggctgtcag aacacaggcg ccaaaccctt cctggagcaa tgtcagcaca
     2041 taaaagatgt cattacccaa aaccagccga taggaaaacc gactataaaa tccttcaagg
     2101 atggcttcga tggtgccgat ggtcagaacc ataaagcccc agaaaatcaa caagtgcaaa
     2161 aaaccggcca gcggatcacg caaaatcttt tgctgggcga tggcgacgct gagcatattt
     2221 ttcaagcgtt cgccggtgtt atcgaggcga ttgcccggtt cggacttgcc aagtttcaga
     2281 atctcaagga ttttgcgact gttgaagaga aacaaactga tcgaagcgag aaaaacagca
     2341 atgaaaatga agtttcctgc agtcatgcgg ggtactccgt cccttctcga attggattgg
     2401 attttgtagg ttccagggta gctgaatgat ggttcattca tcctactata aaccatcatt
     2461 gctcttccag gggcagtatt tgattttttt ccggaattga aactgtcgga gagtttttga
     2521 aacgcggcgc cactatacat cagcccgcat cggttgtcat acgaattatg aagtgtgaat
     2581 tatgcggtat ggattgcgaa actctttgcc tccaaaagag ttaagccata ttttcagtca
     2641 gagaatatct ggaaaaacac tgccggtttg gtgcaatatc ggtctcgtta tccagttttc
     2701 cactggctgc cagggcaaac ccggattcga cttgccagaa gcgcttgtca ccctgtactt
     2761 tagcttttct aagtcagtag aacgtgagtc agtggtcagc agtcaaatcc aggccttcaa
     2821 gaaaaccgcc tgttttcaaa aagacaatcc ataaataaca caaatagaac cacatccgtt
     2881 tgaagccaaa cctaaaaccc taacatttga ctactgactg ctcacttatc aaaacatttt
     2941 ttcttttttc aggaggattt ctatgtttcg tggcgtcaaa acgctcgtga cagcctttgg
     3001 cctgagcctg gctgttgttt tgagcacagg aactgcactg g
//
LOCUS       DAOQDO010000542         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25670_length_3035_cov_3.494295, whole genome shotgun sequence.
ACCESSION   DAOQDO010000542 DAOQDO010000000
VERSION     DAOQDO010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25670_length_3035_cov_3.494295"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKZ53_09740"
     CDS             <1..507
                     /locus_tag="PKZ53_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNJ40759.1"
                     /translation="SIFQALTCQAQAAECRVLVDIPPADLLSVLPAKPWLVKPNHHEA
                     GTALGQPVVTAADAIQAGRELWLRGAQNALITLGAQGAVLLTEAGDVWHLTVPDLSTS
                     GRLFHTGAGDATLAGIACGLQKKHLSLLEAVRFGLASGTTFARYGRTFDPAEIERMVN
                     EIRLKKMG"
     gene            complement(626..1432)
                     /locus_tag="PKZ53_09745"
     CDS             complement(626..1432)
                     /locus_tag="PKZ53_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ40760.1"
                     /translation="MENSRGNLKVRLVIAVVVAVGAIFGYFRSTQKNQITGEVQQVAL
                     TEDQEVALGQQAAPQMLQQYGGESSDPKENARVDQIGNRLVKQSAARNSKYQFEFHVL
                     NDQKTINAFALPGGQIFITRALLSQLKTDGQVAGVLGHEIGHVIGRHGAEHMAKQNLT
                     QGLVSAVGVGADSQQAAQLAAAIGNLVNMKYGRDDELESDTLGVRFMSEAGYNPQAMI
                     GVMEVLAQASKGNRNPEFFSTHPNPENRIDKIKAAIQEKFPQGVPATLEK"
     gene            complement(1502..1942)
                     /locus_tag="PKZ53_09750"
     CDS             complement(1502..1942)
                     /locus_tag="PKZ53_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40761.1"
                     /translation="MKSTQNLELIYQLLCDDVRLELGNKLSFMGVFQDIYVQQLPFQL
                     PRIAVVNHWRGNGKFLSEVRILYPGKQQVVAHSTPTLFEVPEGGYANNITFFMNLAFE
                     RPGEFIVQTLIDSNLFAERRMIVGVVQQAAHPMPESAADDELLN"
     gene            complement(2343..2708)
                     /locus_tag="PKZ53_09755"
     CDS             complement(2343..2708)
                     /locus_tag="PKZ53_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40762.1"
                     /translation="MLLALVFPGHQSLYLSLVGSTDTFEFKTPSHLKKPWSLGVPRQV
                     GALPWLKAAVIRFGKEWAVRFDVIGFNSNYQESLNYKVFDRKSFFISHQEIVPDISGC
                     SWLRMSIKNKTEFNTTNLQ"
     gene            2708..2941
                     /locus_tag="PKZ53_09760"
     CDS             2708..2941
                     /locus_tag="PKZ53_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40763.1"
                     /translation="MAKKRKRKQRTRDTITRAVTHIRLLSLEAANESKRQQLDELGGE
                     YQRVCQAYVTLFCTTHEPDQELSFQIETQLSAR"
BASE COUNT          754 a          724 c          803 g          754 t
ORIGIN      
        1 tccatttttc aggcattgac ctgtcaggcc caggcggctg agtgtcgagt gctggttgat
       61 attcccccgg ctgatctctt gagcgtccta ccggcaaaac cctggctggt caaaccaaat
      121 caccacgaag ccgggaccgc acttggtcag ccggttgtga ctgcggctga cgccatccag
      181 gctggtcgtg aattgtggct ccggggagcg caaaatgcct tgattaccct cggggcacaa
      241 ggggctgtgc tgctgactga agctggtgac gtctggcacc tgacggttcc tgatctctca
      301 acctccggtc ggctgttcca tactggcgcc ggagacgcca cactggccgg aattgcctgt
      361 gggcttcaga aaaagcacct gtcactgttg gaagccgtgc ggtttggtct ggcttcagga
      421 acaacctttg cccggtatgg gcgaacgttt gacccggcgg agattgagcg aatggtgaat
      481 gagattaggc tgaagaaaat gggctgaaaa atcctcgggc ttggggctga agacttgcaa
      541 gctcagggct gaagaaaatg ggttgaggaa ttagggaata gccaggtaat tcaatcaaat
      601 gggttgagtg aactactgac tgatattact tttccaatgt tgcgggtacg ccttggggga
      661 acttttcctg aatggctgct ttgattttat caatccgatt ttcagggttg gggtgggtac
      721 tgaaaaactc tggattacgg ttgccttttg aagcctgagc caggacttcc atcacgccaa
      781 tcatggcctg tgggttatag ccagcttcgg acataaagcg aacgccaagg gtgtcggatt
      841 cgagttcatc atcacgacca tatttcatat tgaccagatt gccgatggcg gcggccagtt
      901 gtgccgcctg ctggctatca gccccaaccc caacggcgga aacgaggccc tgagtgaggt
      961 tttgtttcgc catatgttcc gcaccgtgac gaccaatgac atgtccaatt tcgtgaccca
     1021 gcacacccgc gacctgccca tcggttttca attgtgacag caatgcccgc gtaatgaaga
     1081 tttgcccacc aggaagggca aaggcattga tcgttttttg atcgttcaac acgtgaaact
     1141 caaactgata cttgctgtta cgggcggcag attgcttcac cagccggttt ccaatctgat
     1201 caacgcgggc attttccttt ggatcagacg actcgccgcc atattgctgg agcatttgtg
     1261 gcgccgcctg ttgcccgagt gccacttcct ggtcttcagt cagcgcgact tgttgcacct
     1321 cgccggtaat ctggtttttc tgggttgacc gaaagtaccc aaagatggcg cccacagcca
     1381 ccaccacggc aatgaccagt cgaactttta aatttccccg gctattttcc atacagttgc
     1441 tccaaaaaat attggtgaga ggcttcaaaa acccggaacc cggaacccgg aacccggaac
     1501 cctaattcag caattcgtca tcagcggctg attccggcat tggatgggct gcctgttgca
     1561 cgacgccaac aatcattcgg cgttcagcaa agagatttga atcaatcagg gtttggacaa
     1621 taaattctcc tgggcgttca aacgccagat tcataaaaaa ggtgatgttg ttggcatacc
     1681 caccttctgg gacttcaaac aatgttgggg tcgaatgcgc cacgacttgt tgttttcctg
     1741 gatagaggat gcgtacttcg gacaaaaact tgccattccc ccgccagtga ttgaccacgg
     1801 caatgcgggg aagttgaaat ggaagttgtt gaacataaat gtcttgaaac acacccataa
     1861 agctaagttt attgcccagt tcaagccgta catcgtcaca cagaagttga taaattaatt
     1921 caaggttttg ggttgatttc acagtcaaag actccattgg gttaatcatc aggtcgtgcc
     1981 agtcaggtga tgccaggctc ctcaacgcag gctggctatc ggcatttttg gaaaagattt
     2041 gaagaataag ataaaggctg gtgaaaaacg gcgtttggaa atgcctgaaa actatgccac
     2101 ggcctgccgt gcaagtccag aaacggccca gatatcaaaa attctaccag tcagtcgtca
     2161 gtcgtcagtc gtcagtcgtt cgctaaactg agtgaactga atcagttgac tgtttgctaa
     2221 gtaccttgcc atcaattttc tgagctattg ggtttggtac gtggttgatt tttttccgtg
     2281 ttttccgtgt tttccgtggt gaaaatgtct ggaaattttc ggtgaggtac ttaatgagga
     2341 tctcattgca aattggtagt attgaattca gttttatttt ttatactcat ccttaaccaa
     2401 ctacaaccac ttatatcagg aacaatttct tgatgactga tgaaaaaaga cttgcgatca
     2461 aatactttgt aatttaaact ttcttgataa ttcgaattga accctatcac atcaaacctg
     2521 actgcccatt ctttaccaaa gcggatgact gctgctttca gccagggaag cgctccaacc
     2581 tggcgcggta caccgagcga ccagggtttt ttgagatggg agggcgtttt gaattcgaag
     2641 gtatctgttg aaccaaccag gctcaaatac aaggattgat ggccgggaaa aaccaaagcg
     2701 agaagcaatg gcgaagaagc ggaagcggaa acagcgaacc agggacacca tcacacgggc
     2761 ggtgacgcat atccgactcc tgtcattgga agcggcgaat gagagcaaac gccagcaact
     2821 ggatgagctg ggtggagagt accagcgggt gtgtcaggcg tatgtgacgc tcttttgcac
     2881 aacgcacgag cccgaccagg agctgtcatt tcagattgaa acccaactct cggcgcggtg
     2941 acatcgggat gcgattcgac aggcggcagg gatggcgcaa gggtggcgca gcaatcggga
     3001 gcgggagttg aacgactatc aggagcaggt tggat
//
LOCUS       DAOQDO010000543         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25720_length_3032_cov_3.143433, whole genome shotgun sequence.
ACCESSION   DAOQDO010000543 DAOQDO010000000
VERSION     DAOQDO010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25720_length_3032_cov_3.143433"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..280)
                     /locus_tag="PKZ53_09765"
     CDS             complement(14..280)
                     /locus_tag="PKZ53_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40764.1"
                     /translation="MRAKGNKEMPCIAEMSFPHRSLKKFHPTAEEKKKPAEKILTTER
                     GEGLIMWRAGFILAICTIAGFYAQATQLALQVGYSSVIKEHFPD"
     gene            366..2219
                     /locus_tag="PKZ53_09770"
     CDS             366..2219
                     /locus_tag="PKZ53_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNJ40765.1"
                     /translation="MSTVSPLDKSTRTVIEVESVTIRFAGDSGDGMQLTGTQFTNTAA
                     IIGNDISTLPDYPAEIRAPAGSLPGVSGFQLNFSSHDIRTPGDEPNVLVAMNPAALKV
                     NLADLEEGGILIVNTNEFNKANLEKAHYTTNPLEDGSLAGYRLIQIPLTDLTLNALSE
                     LTMLRKYKERCKNFFALGLVFCLYDRPLEPTMEWINANFGNKPEVAEANRLALKAGLD
                     YADTIELFSTHYRVKKASLKPGKYRKITGNEATALGFVTAAQIAGQPLFYGSYPITPA
                     SEILHELSKLKHFNVKTFQAEDEIAAIGATIGAAFAGNIGLTGSSGPGIALKSEAIGL
                     AVMTELPVVIVNVQRGGPSTGLPTKTEQADLFQAIFGRNSECPAVVLAPASPGDCFHM
                     AIESVRLAIEFMTPVFFLSDGYIANGAEPWKVPALSELPAIKFEYTTNPEGFMPYTRD
                     PNTFSRPWAIPGTPGLEHRIGGLEKQHITGNVCYDPDNHDFMVRLRAQKIANIANFIP
                     EVEVFGEPEGDLLVVGWGGTYGAITTAVETLQHEGHRGSSIHLQYLNPFPRNLGDVLK
                     RFQRVAVAELNLGQLCHILRGRFLIDAVSINKVAGQPFKVSELVRRFRELL"
     gene            2332..2922
                     /locus_tag="PKZ53_09775"
     CDS             2332..2922
                     /locus_tag="PKZ53_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ40766.1"
                     /translation="MSAEPNRWYTLEEYFELERRSDVRFEFVNGQIFAMSGAQPNHNR
                     VTRNIIRRVSNQFEGKTCEVFGSDQRVKVFVASPYLYPDVSIACPKPEFESINGLLAL
                     TNPTVLVEVLSPTTRNDDVKAKFIMSQTIPSLLEYVVVEPDKVKVVHFQKQTDGNWEP
                     ELLEFLTDTLTLPSIDCEVSLTDVYQSVEFSANPIV"
BASE COUNT          740 a          835 c          713 g          744 t
ORIGIN      
        1 tgagagcaac cgctcaatcc gggaagtgtt ccttgatgac tgaagaataa ccgacttgca
       61 aagcgagttg ggttgcctgg gcataaaacc cagcgatagt gcaaatggcc aaaataaaac
      121 ctgcgcgcca cataataagg ccctcccccc tctcggttgt caagatcttt tcagcaggtt
      181 tctttttttc ttcagcagtt ggatgaaact ttttcaagct ccggtgggga aatgacattt
      241 ccgcaatgca aggcatttct ttattcccct tggcacgcac tgttgaattg gcctactatc
      301 caaaattgcg accagctccc accgaactgg cctgatcttt tccaccttcc ggagactgtt
      361 tttatatgtc caccgtttcc cctctcgata aatcaactcg cactgttatc gaagttgaat
      421 ccgtaaccat ccgctttgct ggtgattccg gtgacggcat gcagctcaca ggaacccaat
      481 ttaccaacac cgccgccatc atcggcaacg acatcagtac gttgccggat tatccggcgg
      541 aaatccgcgc cccagccggg agcctccctg gggtttcagg ctttcaactc aacttttcca
      601 gccacgacat ccgcaccccc ggcgacgagc ccaacgtcct ggtcgcgatg aacccggctg
      661 ccctcaaagt caatcttgcc gatttggaag agggcggcat tctgattgtc aacaccaacg
      721 agtttaacaa ggcaaatctc gaaaaagccc actacaccac caacccatta gaagatggat
      781 cgctggccgg gtatcggttg attcaaatcc cgttaaccga tttaaccctc aatgcgcttt
      841 ccgaactgac catgcttcgg aagtataaag agcggtgcaa aaacttcttt gccttgggtc
      901 tggtgttttg tctgtatgac cgcccgcttg agcctaccat ggaatggatc aacgccaact
      961 ttggcaacaa acccgaggtg gccgaggcca accggctggc gctcaaagcc gggctcgatt
     1021 acgccgacac catcgaattg ttttcgaccc actatcgagt caaaaaagcg agtttaaaac
     1081 ctgggaaata ccgcaaaatc accggcaacg aagccactgc ccttggcttt gtgacagcgg
     1141 cacaaattgc aggtcaaccg cttttttatg gcagctaccc aatcacaccg gcttcagaaa
     1201 tcctccacga actgtccaag ctcaaacatt tcaatgtcaa aaccttccag gctgaagacg
     1261 agattgcagc catcggcgcc acgattggag ccgcctttgc cggcaatatc gggctcaccg
     1321 gttcgagcgg ccccggaatt gccctgaaaa gtgaggccat tggactggcc gtgatgactg
     1381 aacttcctgt ggtcattgtc aatgtccagc ggggtggccc ctcaaccgga ttgccaacca
     1441 aaactgagca ggcagattta ttccaggcca tctttggccg caacagcgag tgtccagccg
     1501 tggttttagc tccggcctct ccaggggatt gcttccatat ggccattgaa tcggttcgcc
     1561 tggccattga atttatgacc ccggtattct ttctctctga cggatatatt gccaatgggg
     1621 ccgaaccctg gaaagtcccg gcactttcag aacttccagc catcaaattt gagtacacaa
     1681 ccaatcctga aggatttatg ccctacacgc gtgaccccaa caccttctcg cgcccctggg
     1741 ctattccggg aacacctggg ttggaacatc gaattggcgg cctggaaaaa cagcacatca
     1801 ccggcaatgt gtgctatgac cctgacaatc acgactttat ggttcgcctg cgagcccaaa
     1861 aaattgccaa catcgccaat tttatccctg aagttgaggt ttttggcgag cctgaaggtg
     1921 acctgcttgt ggttgggtgg ggcgggacct atggggccat cacgacggcg gtagaaaccc
     1981 tccagcacga ggggcatcgg ggttcaagta ttcacctcca gtatttaaac ccgtttcctc
     2041 gaaacctggg cgacgtcctg aaacgctttc aacgcgttgc ggtggcggaa ctcaatctgg
     2101 gtcaattgtg ccatatcctt cggggccggt tcctgattga tgccgtcagt atcaacaaag
     2161 tcgctggcca accctttaag gtttcggaac tggtcagacg atttcgtgag ttgctttgat
     2221 caaacccaag ggctgggagc cgaagaagtc gggcgaaaga aattcagctt gtttcctcgg
     2281 aaattttgat tttcattcct ggccctttcc ctgacctcct gttcactcgt tatgtctgct
     2341 gaacccaatc gctggtatac cctggaagaa tatttcgaac ttgaacgccg gtctgatgtg
     2401 cgctttgaat ttgtgaatgg tcagatcttt gccatgtcgg gtgctcaacc aaaccataat
     2461 cgggtaacac gaaatatcat ccgcagagta tccaatcaat tcgagggaaa aacctgcgag
     2521 gtgtttggca gtgaccagcg ggtcaaagtc ttcgtcgcaa gcccatatct gtacccggat
     2581 gtttcaatcg cctgccccaa acctgaattc gaatcaatca atgggctgct ggcgctgaca
     2641 aacccaacgg tcttggtcga agttctttcg cccacaacac gcaatgatga cgtcaaagcc
     2701 aaattcatca tgtcccaaac cattccatcg cttttggaat atgtggtggt cgaaccagac
     2761 aaagtaaaag tagttcactt tcagaaacag accgatggga actgggaacc tgagctgctt
     2821 gaatttttga ctgacaccct cacgcttcct tcaattgatt gtgaagtaag cctgactgac
     2881 gtctatcaat cagttgaatt ttctgccaac ccaattgtgt gaaggttgga agtttttttt
     2941 aattcggaga accaactatg ccgactgaat tgcccaaaat tgcgggtgct gtcgccaacc
     3001 cgttaggtct gaagcgcgct gattttatct cg
//
LOCUS       DAOQDO010000544         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25824_length_3025_cov_2.949495, whole genome shotgun sequence.
ACCESSION   DAOQDO010000544 DAOQDO010000000
VERSION     DAOQDO010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25824_length_3025_cov_2.949495"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..681)
                     /locus_tag="PKZ53_09780"
     CDS             complement(157..681)
                     /locus_tag="PKZ53_09780"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNJ40767.1"
                     /translation="MPGLSTINFKQWIEEHRHLFQPPVGNAQIWKDREFMVTVVGGPN
                     SRKDYHIDPGEEFFYQLEGDMVLRIVEDGVHRDLPIREGDILLLPPFVPHSPQRGPKT
                     LGLVIERIRRPDELDQFQWYCEACGSVLHTVSVHVSDITTQLKPVFDAYWADLDARTC
                     NHCGVVQQPPTPIE"
     gene            complement(896..1342)
                     /locus_tag="PKZ53_09785"
     CDS             complement(896..1342)
                     /locus_tag="PKZ53_09785"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ40768.1"
                     /translation="MNGQAYILHERAKALARYPHMRRVGGLLYVSGTSSRRPDNSFDG
                     VTLKADGTVELDIRAQTRAVIENIRLILREAGADLTHLVDVTVFLVDMKDYTGYNEVY
                     NHYFQAETGPTRTTVAVHQLPHPHLLIEIKAIAIDPAHIQEDRAIL"
     gene            1564..2205
                     /locus_tag="PKZ53_09790"
     CDS             1564..2205
                     /locus_tag="PKZ53_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ40769.1"
                     /translation="MFCPNCGTEERQPSQYCRACGTDMRMVRTSLQRPDSVTAAAVSA
                     RDEIGKAIASKILELRTVKDLSHLAEDVLPEIEKFLESPEERRLRRIRIGITVASVGL
                     GAVVFMLLMSSVMGERIPWPAGFVPLFIGLGILINGWFFTTTPSPRRQEQEFQVDADK
                     QRQLEGGMRFSTSELQLPSGASQMIVPPSITEHTTEMLPPQAREAIPRARNTN"
     gene            2577..2978
                     /locus_tag="PKZ53_09795"
     CDS             2577..2978
                     /locus_tag="PKZ53_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40770.1"
                     /translation="MNNRWVPNNDVSDHVTLERMTMESLSVQGGDPTKGIQLIGELLQ
                     NESVSREEAAQLRCQLAELLELTGRYDEALAAITPVDLTGRQYVLPQTTIANLWYRLG
                     SIYRWLDNAPRSIYCATNALKLYTEIKEFIK"
BASE COUNT          804 a          753 c          686 g          782 t
ORIGIN      
        1 tccgccagct ttttgccact ttttcgtacc cttttaatgg ggacttgtac ttagggatca
       61 ataccttccc agaagcaaga actcagagcc agggattaag aaccaagaac taagaatcaa
      121 gagccaggtg tttcttcatt ctcaattctt cattcttcat tcaattgggg ttggtggttg
      181 ttgaactact ccacaatggt tgcaagttcg ggcgtcaagg tcagcccagt aggcatcaaa
      241 aaccggtttg agctgagtgg tgatgtctga cacatggacg ctgacggtat gcaaaactga
      301 tccacaagct tcgcaatacc attggaactg atccagttca tctgggcgtc gaatccgttc
      361 gatcaccaga cccagggttt tgggaccgcg ctgaggtgaa tgcggcacaa acggcggcag
      421 gaggaggata tctccctcgc ggatgggtaa atcgcgatga acgccatctt cgacaatccg
      481 caacaccata tcgccctcca gttgataaaa gaattcttcg cctggatcaa tatgatagtc
      541 ctttcgacta tttggccccc caactacggt gaccataaat tcccgatcct tccagatttg
      601 ggcatttcca accggtggtt gaaacaaatg gcgatgctct tcgatccact gtttgaaatt
      661 gatagttgat agtcctggca tagaaactcc ttttgaatga agaatgaaga attgagaatg
      721 aagaatgaag aatgaagaaa gtggttagtg cttggtgctt agtgcttagt gcttaggtat
      781 caatactttc gaatatgcac caagaaccaa gcatcaagaa tcagtcacca aaaaccaaac
      841 atcagcttca ttcttcattc ttcattcttc attcttcatt cttcattctt cactcctaca
      901 aaatggcccg atcttcctga atatgagccg ggtcaatggc aatggctttg atttcaatca
      961 agaggtgtgg gtggggtaat tgatgaacag ccacagtggt tcgagtcggt ccggtttcag
     1021 cctgaaaata gtgattatag acctcgttgt acccggtgta gtccttcata tccaccagaa
     1081 agacagttac atcaaccaga tgggtcaggt cagccccggc ttcgcgtaaa atcagccgaa
     1141 tgttttcaat caccgcccga gtttgggcac ggatatcaag ctcaaccgtc ccatcggcct
     1201 tgagcgtgac accgtcaaaa gaattatctg gccgacgtga cgacgttcct gaaacgtaga
     1261 gaagtccccc gacacggcgc atatgcgggt accgggcaag tgctttggca cgttcatgga
     1321 gaatataggc ttgtccgttc ataatatctc aacttggggt gagggtcctg gaagcaggta
     1381 taccaggggc aggaacgaaa atggctttcc cgatatatct ccaaaactac caggtgaaaa
     1441 gatttctcaa gaaggttgct ggacgggcca gcaatttcct attatgggaa ggctattttt
     1501 gcagcctagc ttcattgtgt caccaaacgc aatcaccaga ttgaaaatct tttgagggaa
     1561 gccatgtttt gcccaaactg tggaactgaa gaacgtcaac ccagtcaata ttgccgcgcc
     1621 tgtggaaccg atatgcggat ggttcgcacc tcgctccagc gccctgactc ggttacagca
     1681 gcggctgttt cggcccgtga tgaaattggt aaagcgattg cctcaaaaat ccttgaactt
     1741 cgaaccgtca aagatctctc ccatctggct gaagatgtgc tccctgaaat tgagaaattt
     1801 ttggaatccc ccgaagaacg ccgtctccgg cgcattcgga tcggtattac cgttgcatcg
     1861 gttgggcttg gtgctgtggt ttttatgctt ttgatgagca gtgtcatggg ggaacggatt
     1921 ccctggccgg ctggctttgt cccacttttt attggtcttg gaatcctgat caatggctgg
     1981 ttttttacca ccaccccctc ccctcgccgc caggaacagg aattccaggt tgatgctgac
     2041 aaacaacgac aactggaagg tggcatgcgg ttttcgacat cagaattgca gcttccctca
     2101 ggtgcatccc aaatgattgt tccaccatca attaccgaac acacaactga aatgttaccc
     2161 cctcaggctc gtgaagcaat cccccgcgca cgaaacacaa attaaatcag ggtgccggac
     2221 gtttatggca cgatagacga cctgatcaaa tctgactggc ctggcaagat ctcaatttca
     2281 caacccggtt tctgaatatt gttccccgtc agtgacgtta tttttcatct atttcattcc
     2341 aaaataagaa gttcttcaac ctcgaacctc aaacccaaaa ccccaaatct caatttccag
     2401 tcacgcgctt atttcacact cgattgagct ttgataaact ggcgtttcat caccataatt
     2461 gcttcaccca acctccacca ccctgataac tggaccaggt tttttcggcc agtttcccga
     2521 tgcccgtctg ttgcactggt tgatcacttt tcacggatac cacaaaggat aggtcgatga
     2581 ataatcgctg ggtgccaaac aatgatgtct cagaccacgt cacccttgaa cggatgacga
     2641 tggaaagtct gagtgtacaa ggcggagacc caaccaaagg aattcagtta attggcgaat
     2701 tgctgcaaaa tgaatctgtc tcacgagaag aagcagctca actcaggtgt cagttggccg
     2761 aattactcga acttaccggg cggtatgatg aagcactcgc ggccatcacc ccggttgatt
     2821 taacaggccg ccagtatgtc cttcctcaga ccacaattgc caacttgtgg tatcgcctgg
     2881 ggagtatcta tcgctggctc gacaatgcgc cacgctctat ttattgcgcc accaacgcgc
     2941 tcaagttata taccgaaatt aaagaattta taaaataagg tggaagtttt acaaacaaaa
     3001 accgaatcgt ttaaagtaac cttag
//
LOCUS       DAOQDO010000545         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25853_length_3024_cov_2.840687, whole genome shotgun sequence.
ACCESSION   DAOQDO010000545 DAOQDO010000000
VERSION     DAOQDO010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25853_length_3024_cov_2.840687"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PKZ53_09800"
     CDS             complement(<1..1637)
                     /locus_tag="PKZ53_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNJ40771.1"
                     /translation="MNVFDQFVSRFLNEYNRFHPVNATLLGHHDFNTQLDDLSTRAIE
                     IQRAWLESARQELAAIPIESLDTHRQVDHTIVSNKIFARQLDLDVLRVWRRDPCFYND
                     LATRSIHGLITRPYAPLDERAHAFIARLRQIPALLNLGRQHLEAELSDPPALPLHASG
                     LQPLVGIPHSFVEAALTEFDGGLWFFEHDVTRVLDNVSDSQLRRQGYDANDQVVDLYR
                     DLLTWLETSVRPRSSGSFALGRDVFAAKLRAEEHITTSVEEIVAVATEKLRQTQARMH
                     ELVARIGSCETVWECVKHLSERDQPTVHDVLPAYAHELGAARQFILDQKLVDVPPGEL
                     RMEEMPPFLRDITIAAIERTGPFEKAELPSFFFVTLPDPKACSNEQAEMLRAHSRLMR
                     QTTIVHEAYPGHFIQGLHWALTPSAVRKSFSSMTFVEGWAHYCEQMMAEQGFWDDDPQ
                     LFLFQEFDSLLRIGRVIVATRMHTEGMTVAEAEAFLQTEVWQEPVLARMEAHRYMRDP
                     FVLVYSWGKWQFQSLLEEFRRQQGSNFSLGDFHNQVLA"
     gene            complement(1799..>3024)
                     /locus_tag="PKZ53_09805"
     CDS             complement(1799..>3024)
                     /locus_tag="PKZ53_09805"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNJ40772.1"
                     /translation="MLWSSWLVYEIGKSWPEADADVAEAIDFMEFYAREAVRFGEPIG
                     VTPSPLPNEVNQAFYIPLGVGVVIPPWNFPLAIMAGMTTAAIAAGNTVVLKPSSDTPV
                     IAAKFAELLEQVSLPAGVLNFLPCSGGAVGDFLVEHPKTRFIAFTGSMEIGLRINALA
                     AKRSPGQLWIKRVVAEMGGKDTIVVDETADLDAAAEGIVAAAFGFSGQKCSACSRAVI
                     VEDVYDAVVERVVERTQKLTVGPTTDPRNFTGPVASKSAYASILEYIEIGKGEGRVVA
                     GGGKLEEAGNGYFIQPTVIADVDPQARISQEEIFGPVLALIKAKDFENALDIANNTEF
                     GLTGALYSQRRERLERARVDFHVGNLYLNRKCTGALVDVHPFGGFNMSGTDSKAGGRD
                     YLLLFLQAKSVAEKF"
BASE COUNT          733 a          857 c          781 g          653 t
ORIGIN      
        1 gccaggacct gattgtggaa gtcgcccagt gaaaagttac ttccctgctg acggcgaaac
       61 tcttcgagca acgactggaa ttgccatttt ccccaactgt aaaccaaaac aaacgggtca
      121 cgcatataac gatgagcttc cattcgagcc agcactggtt cctgccagac ttccgtttgt
      181 aaaaaggctt cagcttcagc cacggtcatg ccttcggtgt gcatccgggt ggccacaatc
      241 acccggccaa ttcggagcag cgaatcaaac tcttgaaata aaaacaattg tggatcgtcg
      301 tcccaaaaac cctgctccgc catcatttgt tcgcagtaat gggcccagcc ttcgacaaag
      361 gtcatactgc taaaggattt gcgaacggct gacggcgtca aagcccagtg caacccctgg
      421 atgaaatgcc ccggataggc ttcgtggaca atcgtggttt ggcgcatcag acggctgtga
      481 gcgcgtaaca tttcagcctg ctcatttgaa caggctttgg ggtccggaag cgtcacaaaa
      541 aagaaacttg gtaattcagc tttttcaaat ggaccggtgc gctcaatcgc agcaatggtg
      601 atgtcacgca aaaacggtgg catttcctcc atccgcaact cacccggcgg cacatccacc
      661 agtttttgat ccagaatgaa ctgtcgagcg gcaccgagtt cgtgggcata ggccggaagc
      721 acgtcgtgaa ccgtcggctg gtcgcgttcc gagagatgtt tgacacattc ccacaccgtt
      781 tcgcaggaac caattcgagc caccagctcg tgcattcgcg cctgggtctg acggagtttt
      841 tcagtggcaa ccgcgacaat ttcctcaacc gacgtggtga tgtgttcttc ggcccgaagt
      901 ttggcagcaa agacatcgcg ccccagcgca aaactgcctg agcttcgagg tcgaacgctg
      961 gtttcaagcc aggtgagcag atcccgatag aggtccacca cctgatcgtt ggcatcatac
     1021 ccctgacgcc gtaactgcga gtcagagaca ttgtccagga ctcgggttac atcgtgctca
     1081 aaaaaccaca gaccgccgtc aaattcggtt aaggcagctt ccacaaacga atgtggaata
     1141 ccaaccagcg gttgtaaccc cgatgcgtga agtggcaagg ctggcgggtc actcagttca
     1201 gcttcaaggt gctgccggcc aaggttcagg agtgcgggaa tctggcgcaa ccgggcaata
     1261 aaggcgtggg cacgttcatc aagcggtgca taggggcggg tgatcaatcc gtgaatcgag
     1321 cgcgtcgcca gatcattgta aaagcatggg tcacgccgcc agacccgcaa cacgtccaga
     1381 tcaagctgcc gggcaaagat tttgttggac acaatggtgt gatcgacctg ccgatgggtg
     1441 tccagcgatt caatcggaat cgcggccagc tcctgacggg ctgattctaa ccaggcacgc
     1501 tgaatctcaa tcgcgcgggt tgacaggtca tccagttgag tattgaagtc atgatgcccg
     1561 agcagcgtgg cattgaccgg atgaaaccgg ttgtattcat tcaaaaaacg actgacaaac
     1621 tggtcaaaaa cattcattag gattcagggt tcagggttca gggttcaggg ttcagggttt
     1681 ttgaagacat tgctttctcc tgcctgactt cctcagcctc aatgtcttca gttcaagttg
     1741 aaaaaagacg ggcacagcaa gctgtgccct tccatttttc aaaatctcag cctgccggtc
     1801 agaatttttc agcgactgat tttgcctgca agaacagcaa caaataatca cggccaccag
     1861 cttttgaatc ggtgccagac atattgaagc cgccaaacgg gtgcacatca accagcgcgc
     1921 cggtgcattt gcggttcagg tacagattgc cgacgtggaa atccacccgg gcacgttcca
     1981 accgttcacg acgctgcgaa tacagcgccc cggtcagacc aaattcggtg ttgttggcaa
     2041 tgtcgagcgc attctcaaaa tctttggctt tgatcagtgc gagcaccggg ccaaagattt
     2101 cttcctgtga aatccgcgcc tgcgggtcaa catccgcaat cacggtcggc tggataaaat
     2161 acccgttgcc ggcttcttcg agtttgccac cacccgccac cacgcggcct tcgccttttc
     2221 caatttcgat gtattcaaga atcgaagcat aggcgctctt gctcgcgacc gggccggtga
     2281 agttgcgtgg gtccgtggtg ggaccgactg tcaatttctg ggtgcgttcc acgacgcgct
     2341 caaccacggc gtcatagacg tcttcaacaa tcaccgcacg cgaacaggct gaacacttct
     2401 gacctgagaa cccaaacgcc gccgccacaa tcccttcggc tgccgcatcg agatcagcgg
     2461 tttcatccac gacaatcgtg tcttttccac ccatttccgc caccacgcgc ttgatccaga
     2521 gctgacccgg agagcgtttc gcagccaggg cgttaatccg caagccaatt tccatcgagc
     2581 cggtaaaggc aataaagcgc gtttttggat gttccaccag aaaatcacca accgcaccgc
     2641 cgctgcaggg taggaaattc aaaacaccag ccggaagcga aacctgttcg agcaattccg
     2701 caaatttggc ggcaatcact ggtgtgtcgc tggatggttt gagcaccacg gtgtttccag
     2761 ccgcaatcgc cgccgtcgtc atcccagcca taatcgccag tgggaagttc cagggcggaa
     2821 tcaccacgcc aactccgagc ggaatataaa atgcctgatt gacttcgttg ggtaatggcg
     2881 aaggcgtcac gccaatcggc tcgccaaagc gcactgcttc acgggcataa aattccataa
     2941 aatcaatggc ttccgccacg tcggcatcgg cttcgggcca gcttttgccg atttcataca
     3001 ccagccagga ggaccagagc attt
//
LOCUS       DAOQDO010000546         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25908_length_3020_cov_2.793592, whole genome shotgun sequence.
ACCESSION   DAOQDO010000546 DAOQDO010000000
VERSION     DAOQDO010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25908_length_3020_cov_2.793592"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKZ53_09810"
     CDS             <1..348
                     /locus_tag="PKZ53_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40773.1"
                     /translation="IPTMDVFYLVVAIIHNLLFYSAILLMDHSIFLQMRAIFGGFADW
                     VNIGMTLALLVSFLAGWSWGLLCTQNAWWLYQDNRAFRTFLISGFFLFWPSIQVIILF
                     TLVKVGLIPDYDC"
     gene            complement(414..>3020)
                     /locus_tag="PKZ53_09815"
     CDS             complement(414..>3020)
                     /locus_tag="PKZ53_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ40774.1"
                     /translation="RVYAVAISPDGELIAVGGWTGYSWETRHSVYLFERVTGKLVTRV
                     TGLPDVVHHLAFSHNGDRLAVLMGSGSGLRVYRMSDGARLVRDMDYGGSGNCVDFDRS
                     GWLVTSCDDGLIRLYDDKLKLVTKAEAPGGKRPFSVKFSPDCKRLAVGYTDSTRVDVL
                     AANDLALLYTPDISGVTNGNLGRVAWSNDGLQLFAGGKWNKSGKFWLRCWSERGRGSW
                     VDVPTCGNTILDLAALGQNSVVFGAFDPAWGVISGQGECVRRVTGETADFRSNWDGFQ
                     TNATGTVVQFSFGQRGHMPAVYDLMSRELTPGISASKLVGPRMTAPGLPITDWKDTTS
                     PKLNGVLLELKPNDRSRSLAITPNGNQFLLGSEWALTCYAKDGKVEWQTPTPGVVWAV
                     NVSGNGKLVLTALSDGTIRWYRATDGTELLAFYPHSDRSRWIAWTPSGFYDATPGSDT
                     LLGWQVSSGCDVEGEFYPIGQFRTVYYRPDVVNAIVRTLDEVAAVKEVFSDGGQMARS
                     VDPLKFRPPVVELLAGNDSIPATSGTVGLRYSVRMPSGEPVTRVKALVDGRPVVIEPI
                     SELQPGAATINREIQIPVPARDCEVSLIAENRFAPSQPVSIQVNWKGNDVVAPVTAEV
                     VKPRLWMLSVGVSDYPSKSWKLANAAKDAGDFAETLAHQEGGYYREVHTKVLTNEAAT
                     GETIRQGLQWLKQNAATGDIAVVFLAGHSLNDPLQRYFFLPFDARLDKLATTGVPFLD
                     VKNVIEVLPCKVLFLVDTSHNDAALGTKIKRGTADLAGVATEMAGADSGGVMITSSTQ
                     TTEESGTSSVFVKALVEGFNGKASASRNGTITVSSLTSYIIGRVQELTNGKQTPAAAQ
                     PQSVPDFAIAAK"
BASE COUNT          672 a          883 c          735 g          730 t
ORIGIN      
        1 ataccaacca tggatgtttt ctatctggtt gttgccatca ttcataatct tcttttttat
       61 agtgcgattc tgttgatgga ccattcaatt tttcttcaga tgagagcgat ctttgggggc
      121 tttgccgatt gggtgaatat cggaatgacc ctggcactcc tggttagttt tcttgcaggt
      181 tggtcctggg gtttgctttg tactcaaaac gcctggtggt tgtaccagga taatcgagca
      241 tttcggactt ttttgatcag cgggttcttc cttttttggc catccattca ggtgatcatc
      301 ctttttaccc tggttaaggt tggcctgatc ccagattatg attgttagat cagacaagga
      361 cataaaggac caaagagacc taaatgacct taactcgaag aacccggaac ccatcatttc
      421 gccgcaatcg caaaatccgg cactgattga ggttgtgccg ctgctggggt ttgttttcca
      481 ttggtcaatt cctggacccg cccgatgatg tagctcgtca acgaactcac cgtaatcgtt
      541 ccatttcggc tggcactcgc tttaccgttg aagccttcga ccagcgcttt gacaaagaca
      601 cttgacgtcc ctgattcttc agtcgtttgg gttgatgaag taatcatcac gccgccacta
      661 tcggcacccg ccatttcagt cgcaactccg gccagatcag ccgttccgcg tttgattttc
      721 gtcccaagcg ccgcgtcgtt atggctggtg tcaaccagaa acaggacttt gcacggcagg
      781 acttcgatta cgtttttcac atctaaaaat ggcacgccgg ttgtcgccag tttgtcgagc
      841 cgggcatcaa acggcaggaa gaaataccgc tggagcggat catttaaact atgaccggcc
      901 agaaaaacga cagcgatgtc tccggtggcg gcgttttgtt tgagccattg taatccttga
      961 cgaatagtct cgccggtggc ggcttcattg gtcaaaactt tggtgtggac ttcgcgataa
     1021 tatccacctt cctggtgcgc cagcgtttca gcaaagtcgc cggcatcttt ggcagcgttt
     1081 gccagtttcc agctcttgct tggatagtcg ctcaccccga ccgagagcat ccataaccgt
     1141 ggcttgacga cctctgccgt caccggtgcc acgacatcgt tccctttcca gttcacctgg
     1201 attgagaccg gctggctcgg agcaaaccga ttttcagcaa tcagcgaaac ttcacaatcg
     1261 cgggctggaa ccggaatctg gatttcccga ttgatggtgg ccgctccagg ttgaagttcc
     1321 gaaatcggtt caatcaccac cggacgccca tccaccagcg ctttgactcg ggtcactggt
     1381 tcaccagacg gcatccggac gctgtatcga agcccaacgg ttccggaagt tgctggaatt
     1441 gaatcatttc cagccagaag ctcaacaact ggtgggcgaa acttcaacgg gtcaaccgag
     1501 cgtgccatct ggccaccatc tgaaaaaact tctttgacgg cggcgacttc atccagcgtg
     1561 cgaacaatgg cattgacgac atcaggccgg taatagaccg ttcggaattg tccaattgga
     1621 taaaattcac cttcgacatc acatccagaa ctgacctgcc agccaagcag ggtgtcactc
     1681 cccggagtcg cgtcataaaa tcccgacggt gtccaggcaa tccaccgcga ccggtcagaa
     1741 tgtggataaa atgccagcag ttcggttcca tcagttgccc gataccagcg gatggtgccg
     1801 tcactgagcg ccgtgagcac cagtttccca tttccactga cattgaccgc ccagaccacg
     1861 cctggcgtcg gtgtttgcca ctccactttg ccatcttttg cataacaggt cagcgcccat
     1921 tcgctgccaa gcaaaaactg atttccattt ggggtaatgg ccagcgaccg cgaccggtca
     1981 ttgggtttga gttcgagcag aacaccattt aattttggcg acgtggtgtc tttccaatcg
     2041 gttatcggca atccaggggc cgtcattcgc ggcccgacca gtttggaggc cgagatgcca
     2101 ggtgtcagtt cccggctcat caggtcatag acagccggca tgtgcccacg ctgcccaaaa
     2161 ctgaattgca ccacggttcc ggtcgcattg gtctggaagc cgtcccaatt gctgcgaaaa
     2221 tcagccgttt cgcctgtgac ccgacgcaca cattctccct ggcctgaaat aacgccccag
     2281 gccgggtcaa aggcgccgaa caccacgctg ttctgtccca atgcagccag atccagaatc
     2341 gtattgccgc aggtgggcac atctacccag cttccacgtc cccgttcgga ccagcatcga
     2401 agccagaact tgccgctttt gttccatttc ccaccggcaa acaactgtaa cccatcgttg
     2461 gaccaggcga cccgccccag atttccgttg gtcacaccgc tgatgtccgg ggtatagagc
     2521 aatgccagat cattggctgc cagcacgtcc acccgtgttg aatcagtgta cccaaccgcc
     2581 agccgtttgc agtcaggtga aaatttgacg ctaaagggcc gttttcctcc cggtgcttca
     2641 gctttggtca ccagtttcaa cttgtcatca tagagtcgaa tcaaaccgtc atcacaactc
     2701 gtcaccagcc agccgcttcg gtcaaaatca acacagttgc cgctcccacc atagtccata
     2761 tcccggacca agcgggcgcc atcgctcatt cggtagactc gcaaaccgct cccgcttccc
     2821 atcagtaccg ccagccggtc gccattgtgc gaaaaggcaa gatgatgcac gacatctggc
     2881 aacccggtta cccgcgtgac cagctttccg gtgacacgtt caaacaaata caccgaatgc
     2941 cgggtttccc aggaatatcc ggtccagcca ccgacggcaa ttagttcgcc atcaggtgaa
     3001 attgccacgg catagacccg
//
LOCUS       DAOQDO010000547         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25916_length_3019_cov_3.520918, whole genome shotgun sequence.
ACCESSION   DAOQDO010000547 DAOQDO010000000
VERSION     DAOQDO010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25916_length_3019_cov_3.520918"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PKZ53_09820"
     CDS             <1..1609
                     /locus_tag="PKZ53_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNJ40775.1"
                     /translation="GTDAFLLTAMLAMIVRDHLQDQSFLDQRTIGFEAVRDTLANIPI
                     EEFIACAGVKLADVEQAVALMTGASSCAVRTDLGIEQTLNSTLNSYLRALLYLLTGNF
                     GKPGGNNFHTYLVPLIGHSEEGERQMRSAVTGFPFIANLLPPNILPLEIDTDHPDRTR
                     GLVIDSANPMVTAADTQAYEKAFAKLELLVVIDVAMTETARMAHYVLPASSQFEKWEA
                     TFFNLEFPSNAFHLRKPLLEPLPGTLPEPEIYTRLVQAMGALPAEFPELAAAAKMGRL
                     PFAMASQKYLKQHPELADLAPIILYRTLGPTLPEGAAAAAFLWATAHQFAARNPEAVK
                     RAGHRGDGPMLGEVLFEALLRSRSGVVFSVNEYEDNWKFIRHKDGKIHLEIPEMLAAV
                     RQLSATKNETVSNYPFVLVAGERRSYNANTIYRDPSWRKQDPDGALRIHPEDATQLGL
                     KTGDWVVCESKRGQVQVQVEVHPSVQRGVVTLPHGYGMEHPSNGAGRKRSGARINELT
                     ATHDMDPIAGTPYHKHVAVRISPLNLG"
     gene            1837..>3019
                     /locus_tag="PKZ53_09825"
     CDS             1837..>3019
                     /locus_tag="PKZ53_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40776.1"
                     /translation="MNLALRRIILLVLCFGLFSSSGFAASSPLPSNAQGITFNFYTTE
                     LSNLCYQLDAMAGLEMGSGEVFRELWKNKIGWSAEDEKLLTQWRECRQPYFESVKLGR
                     LPQEPLYQPNPPVQPYVDLSKKIRIASLIATHPDDYQSALAVTVLPADAYRLAAILET
                     FQLRFHPWWETEGKRITEPFVQQFTDLMAQKNLTEFSNQVARFYESQIAPGYAINIFL
                     MARPKSKNQHTSGEVFDNFSTVEILEGEKPEQRIDVVLHEVFHHFFSLSPVEKRIALV
                     NAFATHPDPDSLAVYHILDEVLATALGNGLVFQKVSTSQYFEKFVAIDQSFYNDPFID
                     KTAKAIMPAIRKRVESGQSLFDGFVDEYISTARQVLGNTMSDPQLRLFVRTSVFADDG
                     LR"
BASE COUNT          704 a          796 c          774 g          745 t
ORIGIN      
        1 tggaaccgat gcctttttgc tgacagcgat gctggccatg atcgtgcgcg atcacttgca
       61 ggatcaatca tttctggatc agcgcaccat cgggtttgaa gccgttcgtg atacgctggc
      121 caatattcca atcgaagaat ttattgcctg tgctggggtc aagctggcag acgttgagca
      181 ggcggtggct ttgatgaccg gggcgtcaag ttgcgccgtt cgcaccgacc ttggaatcga
      241 acaaacactc aacagcacgc tcaattccta tttgcgggcg ctgctctatt tattgacggg
      301 caattttggg aaaccgggcg ggaataattt ccatacctac ctggtaccgt tgattggtca
      361 ttccgaagag ggtgagcgcc aaatgcggtc agcggtcacg gggtttccat tcatcgccaa
      421 cctgttgcct cccaatattt taccgctcga aattgatact gatcacccgg atcgtacccg
      481 aggactggtg attgatagtg ccaacccgat ggtgacggcg gcagataccc aggcgtatga
      541 aaaagcgttt gccaaacttg agttgctggt ggtgattgat gtggccatga ccgaaaccgc
      601 ccgcatggcg cactatgtgt tgccggcttc gtcgcagttt gaaaaatggg aggctacatt
      661 ttttaacctt gagttcccga gtaatgcctt tcatttgcgc aaaccattgc tggaaccact
      721 ccctggaacc cttccggaac cagagattta cacccggctt gttcaggcaa tgggcgccct
      781 tccggctgaa tttcccgagc ttgctgccgc cgcaaaaatg ggtcgtctcc cctttgccat
      841 ggcctcccaa aaatacctca agcagcatcc ggaactggcc gatctggcac cgatcatttt
      901 gtatcggacg ctcggaccga ccctccctga gggcgcggcg gcagcggctt tcctgtgggc
      961 aaccgctcat cagtttgcgg cacgaaaccc tgaggccgtc aaacgcgccg ggcatcgcgg
     1021 agacgggcca atgcttgggg aagtgctctt tgaagccctg ctccgctcgc ggtcaggtgt
     1081 tgtctttagc gtcaatgaat atgaagacaa ctggaagttc attcgtcaca aggacgggaa
     1141 aatccacctg gagattccag agatgctcgc agccgttcgt cagctttcag ccacaaagaa
     1201 cgagacagtc tctaactatc catttgtcct ggtggctggt gaacgccgtt cctataacgc
     1261 caacaccatc tatcgtgacc catcctggcg caaacaggat ccggacgggg cgcttcggat
     1321 tcatcccgag gatgccaccc agttgggcct caaaactgga gattgggtgg tgtgtgagtc
     1381 caaacgcgga caggttcagg ttcaagtcga agttcaccca tcggttcagc gtggcgtggt
     1441 cacgctcccg cacggatatg gaatggaaca tccttccaat ggcgccgggc ggaaacgatc
     1501 tggcgcccgc atcaacgaac taaccgccac ccacgatatg gatccgattg ccggaacgcc
     1561 atatcacaaa cacgtcgcag tgcggatttc tcctctcaat ttgggttgaa gaagtcaggc
     1621 tgagggcttg gggctgaaga tatggggctg aaggtattaa acccgttgtc ttcagccccc
     1681 gagctggcga gtcgttctcc cctgagccct gagccatggt tctgtcggcc ctaaaaaaat
     1741 ggaactctcc aggcataaca ggttgtaatg aggttgctgt ttgcgctccc ccaacctcaa
     1801 catgaaatca aattcactct gtacctggag aaacttatga atctggcttt gcgccgaatc
     1861 atcttgcttg tcttgtgttt tgggctgttt tcaagttctg ggtttgccgc cagttccccg
     1921 cttccatcaa atgcgcaggg aattaccttt aacttttaca ccactgaact ttccaacctg
     1981 tgctatcagc ttgatgccat ggctggcctg gaaatgggct caggcgaagt atttcgcgaa
     2041 ctgtggaaaa ataaaattgg ctggtcagcc gaagatgaaa aactccttac ccagtggcgt
     2101 gaatgccgtc aaccgtattt tgaatcagtc aaacttgggc gactgcccca ggaaccactc
     2161 tatcaaccca acccaccggt ccagccgtat gttgatttga gcaagaaaat tcggattgcc
     2221 agtttgattg caacccatcc tgatgattat caatcagccc tggcggtgac ggtccttcca
     2281 gccgatgcgt accggttagc agctattctt gaaacctttc aacttcggtt tcatccgtgg
     2341 tgggaaaccg aaggaaaacg aatcactgaa ccgtttgtcc agcaatttac tgacctcatg
     2401 gctcagaaaa acctgactga attcagtaat caggtggcgc ggttttatga gtcccaaatt
     2461 gcaccgggtt atgcaatcaa tatttttttg atggcgcgtc caaaatccaa aaaccagcac
     2521 accagcggtg aagtgtttga caatttttca acggttgaaa tcctggaagg cgaaaaaccc
     2581 gagcagcgaa ttgatgttgt gctccacgaa gtcttccacc atttctttag cttatcgccg
     2641 gttgaaaaac gaatcgctct ggtcaatgcg tttgcaaccc accctgaccc ggattcgctg
     2701 gctgtgtatc atattctgga tgaagtgctg gcaacggctc tgggcaatgg cctggtcttt
     2761 caaaaagtct caaccagcca gtattttgaa aaatttgtcg caattgacca gtcgttttac
     2821 aacgacccgt ttattgataa aacggccaaa gccatcatgc cagcaattcg aaagcgggtt
     2881 gaatcaggcc agtcgctctt tgatgggttt gttgatgaat atatctcgac ggcacgtcag
     2941 gtactgggta acacgatgag tgacccgcag ttgcggctgt ttgtccggac cagcgttttt
     3001 gccgacgatg gcttgcgac
//
LOCUS       DAOQDO010000548         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25927_length_3018_cov_4.059737, whole genome shotgun sequence.
ACCESSION   DAOQDO010000548 DAOQDO010000000
VERSION     DAOQDO010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25927_length_3018_cov_4.059737"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1508)
                     /locus_tag="PKZ53_09830"
     CDS             complement(105..1508)
                     /locus_tag="PKZ53_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40777.1"
                     /translation="MKFFVPSFRQSVCVMGLTSALIISSLGEPGILAQSKPQTPPGSV
                     AKAPVTPVESVDKAAVLAATEEIFKAVSKLRGLEIKKDVKAGFRNREELEKDVLKDMD
                     ESTPASEFDAQAKLLGKLGLVPAKYKLREEMIKVLSEQIGGYYKPRTGEFFLTDRTDL
                     EEQKIVIAHELTHALQDQHFDLRRFENFPKGMGDKESAIHALIEGEATVVMFDYLLEP
                     LGGSVSKLPISITDMLQVMSGPGMDSQEKTQMLSKAPRCIRESLTFPYFTGAGFVQAL
                     VKEGGWASVSKAYTTLPESTEQIMHPEKYLKGESATRITLAPAESVLGKDWKVVTGDV
                     TGEFGYTLILSEYLDEADGSRGADGWDGDACRLYENAKTGQLLLVQHTSWDSADEAKE
                     FYDAYTERTRKRYAGSKFVETKQGFQGETKEGWVTVQIKGSEVLVLEGSTTAKDASTA
                     SAWFWKEDKCEKTKKQG"
     gene            complement(1732..>3018)
                     /locus_tag="PKZ53_09835"
     CDS             complement(1732..>3018)
                     /locus_tag="PKZ53_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ40778.1"
                     /translation="QQMARTMRWQGIHQWLKPGITLWFVALNIPSFWILGWLLSHGIP
                     YPYLAGPAIDRLPTLVALPFTVWQWSSLACIGVLITTQISHWLWKKSQIWRARRRLAI
                     RATAKEQPAAPAVSQPVPQVLEAVPTPIPTSVLPDEATPLIPRPAVGRREFIQAASLA
                     LTTSPFLVSTYGAYIARESYRLERLSLTLPGMPPELRGTTIVQLTDVHAGVFMDHHRL
                     MEFVDVANGLNPDLIVLTGDYVPYSSTCVPLFIGAFRHLRARYGVYATLGNHDLFTNS
                     VDALAEGFRNHNIRLLINEQETIWIRGAKLNLMGITYIPQSRHERLADLTFDAAMRRL
                     EVRDTSILLSHQPNVFPKALERNIPLTISGHTHGGQVKVEFLGQDIAPSRLMTDFTAG
                     LYQLDGSQLYVSRGVGTSGPPVRLGSVPEITQITLV"
BASE COUNT          697 a          811 c          765 g          745 t
ORIGIN      
        1 ccctagccct ggttttttca gccctgagcc cctagccctg gttttttcag ccctgagccc
       61 ctagccctgg ttttttcagc cctgagcccc tagccctggt tttttcagcc ctgttttttt
      121 gttttctcgc atttatcttc tttccagaac caggcagatg cagtactggc atcttttgcc
      181 gttgttgatc cttccagaac caggacttct gagcccttta tctgcacagt gacccagcct
      241 tctttggtct cgccctgaaa tccctgtttg gtttcaacaa acttgctgcc tgcatagcgt
      301 ttgcgcgttc gttcggtata ggcgtcataa aattctttgg cttcatcggc tgagtcccaa
      361 ctcgtgtgct ggaccagaag caactgcccg gttttggcgt tttcatacaa ccgacaggca
      421 tcaccatccc agccatcagc accacgtgag ccatcggctt catccagata ctcagacaga
      481 atcagggtat agccaaactc gccggtcaca tctccggtga cgactttcca gtcttttcca
      541 agcacgcttt cggctggggc cagtgtaatc cgggtggccg actcaccttt cagatacttt
      601 tcagggtgca taatctgttc ggtgctttcg ggcaacgtgg tataagcctt tgaaacactg
      661 gcccatccac cttctttgac cagtgcctga acaaacccgg caccagtaaa ataaggaaag
      721 gtcaacgatt cacgaatgca tcggggcgcc ttcgacagca tctgggtttt ttcctgcgaa
      781 tccatcccag gacctgacat cacctgcaac atatcggtga ttgaaatcgg gagctttgaa
      841 acactgcctc ccagtggttc cagcagataa tcaaacatca caacggtggc ttcgccttca
      901 atcagggcgt gaatggctga ttccttatcg cccatgcctt tgggaaaatt ctcaaaccgg
      961 cgcaagtcaa aatgctgatc ctgcaaggca tgcgtcaatt cgtgggcaat cacaatcttc
     1021 tgttcttcca aatcagttcg gtctgtcaga aaaaattcac ccgtgcgcgg cttgtaatac
     1081 ccgccaattt gctcactcag aactttgatc atttcttcgc ggagcttgta tttggctggc
     1141 accagtccaa gtttaccgag caatttggcc tgggcatcaa actcgctggc cggcgttgat
     1201 tcatccatgt ctttcagaac gtccttttcc agttcttcac ggttgcgaaa accagctttg
     1261 acatcctttt tgatttcaag tccacggagc ttgctcaccg ctttgaagat ctcctcagtt
     1321 gcagccagta cggcggcttt atcaactgat tcgactggag ttactggtgc tttggcaacc
     1381 gaaccgggcg gagtttgtgg tttggactgc gccaaaatgc cgggttcccc cagtgacgaa
     1441 attatcaagg cagaagtcag gcccatcaca cagacggact gacgaaagga cggaacaaaa
     1501 aatttcacgg aagacctcca gggtgatgct tgttttttgg tgcttggtgc ttggtgcttg
     1561 gtgcttggtg cttggtgctt ggtgcttggt gcttggtttc gaaaacccgg aaccctgaca
     1621 agatgactga ctgacaagat gatcgggtga tttttttcat ccctcatcct tcatccctca
     1681 tccctttaga aacccggaac ccggaacccg agatactgag tccggcctgg cctacactaa
     1741 cgtgatctgc gtaatttccg gcacggaacc gagcctgacc ggtgggccgc tggtaccaac
     1801 gccccggctg acatacaact gagagccgtc aagctggtaa agaccagccg taaaatctgt
     1861 catcaaacga cttggggcaa tatcctggcc gagaaattca actttgacct ggccaccgtg
     1921 ggtatgtccg gagatggtaa gtgggatatt gcgttccagg gcttttggga aaacgtttgg
     1981 ctgatgggaa agcaaaatgg atgtgtcgcg cacttccagt ctccgcatcg ccgcgtcaaa
     2041 agtcaaatcc gccaggcgtt cgtgacgtga ttgaggaatg taggtaattc ccatcaagtt
     2101 cagtttggca ccgcggatcc agatagtttc ctgttcattg atgagtaatc ggatgttgtg
     2161 attacggaag ccttcggcca gcgcatccac cgagttggta aacagatcgt gattcccaag
     2221 agtggcatag actccgtagc gagcccggag atgtcgaaaa gcaccaataa aaagtggaac
     2281 acaggtgctg gaatacggca catagtcacc ggtcagtaca atcaagtcag gattcagccc
     2341 attggcgacg tctacaaact ccatcaaccg gtgatgatcc ataaacacac cggcgtggac
     2401 atcggttaac tgcacaattg tcgttccacg caactccggc ggcattccgg gtaatgtcaa
     2461 cgataaccgt tcaagccggt aactttcgcg ggcaatatag gcgccatagg ttgagaccag
     2521 aaacggagac gtggtcaatg ccagcgatgc ggcctggata aactctcgcc gcccaactgc
     2581 cggacgtgga atcaggggcg tggcttcatc tggaaggaca ctggtgggaa ttggggttgg
     2641 aacggcttcc agaacctggg gaacaggttg agaaacggcg ggcgcggctg gttgttcttt
     2701 ggcggtagcc cggatagcca atcggcggcg ggcccgccag atttgacttt tcttccaaag
     2761 ccagtgactg atttgggttg taatcaggac tccgatacag gccagcgaac tccactgcca
     2821 gactgtaaac ggcaacgcaa ccagggttgg caaccgatct atcgccgggc cagccagata
     2881 cggatatgga atgccgtgag acagcaacca tcccaaaatc cagaacgagg gaatattcaa
     2941 agccacaaac cagagggtaa tgccgggttt gagccactgg tggattccct gccagcgcat
     3001 cgttctggcc atttgctg
//
LOCUS       DAOQDO010000549         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_25953_length_3017_cov_2.386901, whole genome shotgun sequence.
ACCESSION   DAOQDO010000549 DAOQDO010000000
VERSION     DAOQDO010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25953_length_3017_cov_2.386901"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKZ53_09840"
     CDS             complement(<1..1060)
                     /locus_tag="PKZ53_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004505476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40779.1"
                     /translation="MQANLIASLVSNLQDYAFLLLDHKGRILTWNSNAEKITKYTADQ
                     VCGQYLTYLQPSGHKTDLESEYDFITRFPQSQVEGERWFQRADHQLFLGYLAVAPVHD
                     TTGTLTNFAVMIHNITHYLHTEDRFRVAVEAAPNAMVMVDKQGKIVLVNSQTEILFGY
                     PRQELIGQRVEILVPERYRQSHPTYRNDFFSSPQARPMGAGRDLYGRRKDGQEMAIEI
                     GLNPLITEEGSFVLASIIDITERKRAEEKFRVAVEAAPNAMVMVDKQGKIVLVNSQTE
                     ILFGYPRQELINHHLEMLVPERYRQGHPTYRNDFFINPHARPMGAGRDLYGRRKDGQE
                     IAIEIGLNPLITEEGAFVL"
     gene            1254..2042
                     /locus_tag="PKZ53_09845"
     CDS             1254..2042
                     /locus_tag="PKZ53_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNJ40780.1"
                     /translation="MKLIDIGINLTHRSFESDREAVLRRAREAGVTTLILTGTSVKGS
                     REALRLARQHPHTLFSTAGVHPHDARHCTDETIEILRKLAESSEVVAIGECGLDYNRD
                     FSPRPDQNRWFEAQIKLASEVKKPLFLHERDAHTAFVQILKPYRDQLTAGVVHCFTGT
                     EAELKAYLDLDLHIGITGWICDERRGQHLQKLVKLVPLNRLMVETDAPFLIPRNLHPK
                     PRDTRNEPAYLPHIVHTIARCLGKTPEEVAAETTATATAFFQLS"
     gene            2269..>3017
                     /locus_tag="PKZ53_09850"
     CDS             2269..>3017
                     /locus_tag="PKZ53_09850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNJ40781.1"
                     /translation="MFIDSHAHIDGQLYDEDRTAILARAAEVGIELILEICNGDLEQG
                     SLEKGLALAAAHSHIYAAVGVHPHDARLYDDELEARLCQMCEHPKVLAWGEIGLDYYY
                     DHSPRDVQQAAFVRQMERARERNLPILLHIRDADEDTIELLREHWSDRGNRAGIFHCF
                     SGSAKLAQAGLDLGFLISFSGNLTFKNAQPLRDIAKSLPLESILIETDCPYLAPVPYR
                     GKRNEPAYVIEVARQLAQLHQTTIEEVGRITT"
BASE COUNT          716 a          781 c          762 g          758 t
ORIGIN      
        1 ccaggacaaa ggcaccttcc tcggtaatca gcggattgag gccgatttca atagctattt
       61 cctggccatc ttttcgccgt ccatagagat cacgccccgc gcccattggc cgggcatgcg
      121 ggttgataaa aaaatcattg cggtaggtcg gatgaccctg gcggtagcgc tcgggcacca
      181 gcatttcaag gtggtgattg attagttcct gccgtgggta gccaaacagg atttcagttt
      241 gcgaattgac cagtacgatt ttgccttgtt tatcaaccat caccatggca ttgggggcgg
      301 cttcgaccgc cactcgaaat ttttcctccg cacgtttgcg ttcggtgatg tcaatgattg
      361 aagccaggac aaacgaacct tcttcggtaa tcagtggatt gaggccaatt tcaatggcca
      421 tttcctggcc atctttgcgg cggccataca gatcacgccc ggcgcccatc gggcgggcct
      481 gcgggctgct aaaaaaatca ttgcggtagg ttggatgact ctggcgatag cgttctggta
      541 ccaggatctc aacccgctga ccaatcaact cctgtcgcgg atacccaaac aagatttcag
      601 tttgcgagtt gaccagtacg attttgccct gtttatcaac catcaccatg gcattggggg
      661 cggcttcgac cgctacccgg aaccgatctt cggtatgcag gtaatgggta atattgtgaa
      721 tcatgaccgc aaaattggtc agtgtgccag ttgtgtcgtg tactggtgcc actgccagat
      781 acccaagaaa aagctggtgg tcggcccgtt gaaaccagcg ttctccctca acctgtgact
      841 gcgggaagcg cgtaataaaa tcatattctg attcgagatc ggttttgtgc cccgatggct
      901 gcaaataggt cagatattga ccacacacct ggtcagcggt gtatttggtg attttttcag
      961 catttgaatt ccaggtgagg attctgccct tgtgatcaag caggagaaat gcatagtctt
     1021 gtaaatttga caccagcgat gcaatgagat tggcttgcat aattacctca cctgggaaag
     1081 gatctttttc agaattggat gaaagaaaag aagaacatca tgtcagtttt acttattcta
     1141 tcaaatataa acccaatttt aataacagtt agaatttctc taagataaca tgcctttaca
     1201 cgttagatga aagtgataga gcccctggca acaggaacca aactaaaatc tctatgaaac
     1261 ttattgatat tggaatcaat ttaacccatc gctcatttga atccgaccgg gaagcggtgc
     1321 tcaggcgagc gcgtgaagcc ggtgttacca ctctgattct gaccggcacc agcgtcaagg
     1381 gcagtcggga ggcgttgcga ctggcccggc agcatccaca caccttgttt tccaccgccg
     1441 gagttcatcc ccacgatgcc cgtcattgca cggatgaaac cattgaaatc ttgcgaaaac
     1501 tggccgagtc gtctgaagtc gtggcgattg gggaatgtgg cctggactat aatcgtgatt
     1561 tttcaccgcg tcctgaccag aatcgctggt ttgaagccca gatcaagctt gcctccgagg
     1621 tcaaaaaacc cttgtttttg cacgaacgcg atgcccatac ggccttcgtt caaattctca
     1681 aaccgtatcg tgaccagttg accgccggag tggttcactg ctttacgggt acggaagcag
     1741 agttaaaagc ctatctggat ctggatttac atattggtat caccggctgg atttgcgacg
     1801 aacgacgtgg acagcacctg caaaaactgg tgaaactggt gccgctcaat cggttgatgg
     1861 tcgaaactga cgctccgttt ttgattcctc gcaacctgca tcccaaaccg agagacaccc
     1921 gcaacgaacc agcctacctg ccgcacattg tgcacaccat tgcgcgctgt cttggaaaaa
     1981 ccccggaaga agttgccgct gaaacgacag cgacggcgac ggcatttttt caattgtcat
     2041 agggttccgg gttccgggtt ccgggttccg ggttccgggt tttcgaattc attctttttc
     2101 cgtgggcttc gcaccacggc tattgaacaa tgatcctgcg ggcctcaaac ccttatcctg
     2161 gcgcttatga ccctgaaccc tgaaagatga tcgggtgatt tttttcatcc ctcatccttc
     2221 atccctcatc ccttttgaaa accctgaacc ctgaaccttc aaggtactat gtttattgat
     2281 tcacacgccc acattgacgg gcaactctat gacgaagacc ggaccgccat cctggcgcgt
     2341 gcggctgagg ttggcattga attgatcctt gaaatttgca acggcgattt agagcaaggg
     2401 tcattggaaa aagggttggc gctggctgcg gctcattcac atatttatgc ggcggtgggg
     2461 gttcaccctc acgatgcccg actctatgac gatgaacttg aagcccgcct ctgccagatg
     2521 tgcgagcatc ccaaagtgct ggcctggggc gaaatcgggt tggattacta ctatgaccat
     2581 tcgccacgcg atgtgcagca agctgcgttt gtccggcaaa tggaacgtgc ccgcgaacga
     2641 aacctcccga ttttattgca catccgggat gctgatgagg acacaatcga gttacttcgc
     2701 gagcactgga gtgaccgggg caaccgggct ggaatttttc attgcttcag tggttcggca
     2761 aaactggccc aggccgggct ggatttgggg tttctgattt cattttcagg caatctcacc
     2821 tttaagaatg cccaaccgtt gcgcgacatt gccaaatcgc tcccgcttga gtccattttg
     2881 attgaaactg actgcccgta tctggcgccg gttccatatc gtggaaagcg caacgaaccg
     2941 gcctatgtga tcgaagttgc ccgccaactg gcccaactcc accaaacgac gattgaagag
     3001 gtgggccgca tcacaac
//
LOCUS       DAOQDO010000550         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26022_length_3012_cov_3.978695, whole genome shotgun sequence.
ACCESSION   DAOQDO010000550 DAOQDO010000000
VERSION     DAOQDO010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26022_length_3012_cov_3.978695"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKZ53_09855"
     CDS             <1..569
                     /locus_tag="PKZ53_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ40782.1"
                     /translation="QGKVRYLGCSNFAARHLAKALVLAGERGWDRFVSLQTYYSLVGR
                     DIEHELLPLCREEGIGVMPWSPLSGGFLTGKYRREGVVETGGRRTRGAFPPIDEDRGY
                     DAVEALDAIAKDRGVTIAQVALAWVLAQKGITSVIIGANKMSQLNDNLKAIEIQLTSE
                     EIATLSATTQPPTQYPGWMYIHQNQNRL"
     gene            755..2155
                     /locus_tag="PKZ53_09860"
     CDS             755..2155
                     /locus_tag="PKZ53_09860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ40783.1"
                     /translation="MIGKMIAQYRIVEALGKGGMGTVYKAQDTQLNRTVVLKLLSQNL
                     LGNDKARSRFLREARLASALDHPNICTIYEIQETGGYYYIAMQYIEGQTLKQAINRRP
                     LSNSTFLSIALQVADALASAHDRGVIHRDIKPQNIMITPRGQAKVLDFGLAKNINESS
                     GAASGAMVSGDLTDQGKTLGTPSYMSPEQARCERLDRRSDIFSFGTVLYEMITGQKAF
                     AGSNTVDIMYGVCNQDPKPISDLNSKAAPGFQELLARAMAKDPNRRYQSMQALITDLK
                     HITATTGGRVGIPDGLHQPFAPVERAGGWMDRLVPPAIRRVFFKSSASGSAELPPQRD
                     SSQQVLPSNESRSVQGADFSIHDDFPLIPGQHKTLAVLPFRNLGGSIDDSARVSLLEA
                     LIIELTKFKSLTIRPTSMVLQYLEEETNPTEVGRDLGVDAVLLGSCLGSANRMRVTTQ
                     LIDIIPATFSGLEKLM"
     gene            2188..2847
                     /locus_tag="PKZ53_09865"
     CDS             2188..2847
                     /locus_tag="PKZ53_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ40784.1"
                     /translation="MCQRIVGELSKKAAGSAFDLLVDEDQDVRMDAISALKFSNDPKA
                     IEALAEALKDRSQLVKAAAADALACFGRRATPILSLEIEDALARQDMGTARYGVQAAG
                     RIQSSELVPILLEALVSDDSFVASEAALALGNIRDQRACSDLVEALSRADANIRFAAV
                     QALGLIADPQSLAPLENRLRQDEDEGVQAKALWAINRIRRKPFGTQTLTIVHTPLLQR
                     H"
BASE COUNT          773 a          742 c          839 g          658 t
ORIGIN      
        1 gtcagggcaa agttcggtat ctgggatgct caaattttgc tgcccggcat ctggcgaaag
       61 cgctggtcct ggccggtgag cgtggctggg atcgctttgt gtcgctccag acctattatt
      121 cactcgtcgg gcgcgacatc gagcacgagt tgcttccgct gtgccgtgag gaaggcattg
      181 gggtgatgcc ctggtcaccg ctttcaggcg gatttttaac tggaaaatat cgccgcgaag
      241 gcgtggtgga aaccggtggc cggcgaaccc gtggcgcatt tcctccgatt gatgaagatc
      301 gtgggtatga tgccgttgag gcactcgacg ccattgccaa agatcgaggg gtgaccattg
      361 cccaggttgc cctggcctgg gtcttagccc agaagggaat tacttcggtg atcattgggg
      421 caaataaaat gtcccagttg aacgacaatt tgaaggcgat cgaaattcaa ttgacgtctg
      481 aagaaattgc cactttgtca gctaccaccc agccaccaac ccagtatccg ggatggatgt
      541 acatccatca aaatcaaaat cgtctgtaac tgaaaatggt caaccgtcag tgaatggtat
      601 gcacaaggca catcgggaca attgattgtc atgtgtagca acgaaaattt ccagccttga
      661 ttcaaagaaa cgcctcaggt ggaatatcac ttgctttatg ggttttacaa tggattgaga
      721 atgaactgac ggttgatcaa aaggagaaca acagatgatc gggaaaatga tcgcacagta
      781 ccgcatcgtc gaggcgcttg gaaaaggcgg catgggcacg gtgtataagg cccaggacac
      841 ccaactcaat cggacagtgg tactcaaact gctgtcccag aacctccttg gtaatgataa
      901 agcccgaagc cggtttttgc gcgaggcccg actcgcctca gccctcgatc acccaaacat
      961 ctgtaccatc tatgagatcc aggaaactgg gggctattac tacattgcga tgcaatacat
     1021 cgagggacag acactgaaac aggcgatcaa ccgacgcccg ctgtccaatt cgacgtttct
     1081 ctccattgcg ttgcaggtag ccgatgccct ggccagcgcc catgatcgag gcgtgatcca
     1141 ccgggatatc aaaccgcaaa acatcatgat caccccgcgt gggcaagcca aagttcttga
     1201 ttttggtttg gcgaaaaaca tcaatgaatc aagtggcgca gcgtcggggg caatggtgag
     1261 cggagactta accgatcagg gcaaaacgct tggaacgcct tcctacatgt ctccagagca
     1321 ggcccggtgc gaacgacttg accgcagatc cgatatcttt tcatttggca ccgtactcta
     1381 tgaaatgatc acgggccaga aagcctttgc cggcagcaat accgttgata ttatgtatgg
     1441 ggtttgcaac caggatccaa agccgatttc agacctgaac tccaaagcgg cgcctggctt
     1501 tcaagaacta ctggcccgag ccatggccaa agatccaaac cgccggtatc agtcaatgca
     1561 ggcgctgatt actgacctga aacatatcac ggcgacgaca ggaggccggg ttgggattcc
     1621 agatgggttg catcagccgt ttgcgccagt cgaacgggct ggtgggtgga tggatcgcct
     1681 ggtcccgcca gccattcggc gggtgttttt caaaagcagc gccagcggat cggctgaatt
     1741 acctccccag cgggattcga gtcagcaggt cctgcccagc aacgaatcgc ggtctgtcca
     1801 gggggcagat ttttcgatcc acgatgattt tccactgatt ccaggccagc ataaaacgct
     1861 ggcggtgctt ccgtttcgga acctgggcgg atcaattgat gacagcgccc gggtctcgct
     1921 cctggaagcg ctgattattg aattgaccaa gtttaaatcg cttaccattc gcccaacgag
     1981 tatggtgttg cagtatctgg aagaagaaac caatccaacc gaagttggtc gagatctggg
     2041 tgttgatgcg gtgttactcg gcagttgtct ggggtcagcc aatcggatgc gagtcacgac
     2101 tcaactcatt gacatcattc cggcaacatt ctctgggctg gaaaaattga tgtaacgaac
     2161 aatgatccaa tcagattact ggatcagatg tgtcagcgga tcgtgggtga attgagtaag
     2221 aaagcggcgg gttcggcctt tgaccttctg gttgatgaag accaggacgt acgcatggac
     2281 gcgatttcag cactcaagtt ttccaatgac cccaaagcaa ttgaagccct ggctgaagcg
     2341 ttaaaagacc ggagccaact ggtcaaagct gccgctgccg atgcgctggc ctgttttgga
     2401 cgccgggcga ctccgatttt gtcacttgaa attgaagacg cgctggcccg ccaggacatg
     2461 ggaacggccc ggtatggtgt tcaggccgca gggcggatcc aaagtagtga actggtgccg
     2521 attttgctcg aagcgctggt ttcggatgat agctttgtcg ccagcgaagc ggcactggca
     2581 ctggggaata ttcgcgacca gcgcgcctgc tcagatctgg tcgaagccct gtcccgagct
     2641 gatgccaaca tccggtttgc tgccgttcag gcactcgggc tgattgcgga tccgcaatcg
     2701 ctggcaccac tcgaaaaccg tctccgccag gatgaagacg agggagtcca ggccaaagcc
     2761 ctctgggcca tcaatcgaat ccgccgcaag ccctttggaa ctcagaccct gaccattgtg
     2821 cacacaccat tgctccaacg gcattgattg gccgaatcaa gaattgagaa tgaagaattg
     2881 agaatgaaga atgaagaatg aagaatgaag aatgaagaat tgagaatgaa gaatgaagaa
     2941 tgaagaattg agaattgaga atgaagaatg aagaattgag aattgagaat gaagaatgaa
     3001 gaatgaagaa tt
//
LOCUS       DAOQDO010000551         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26059_length_3010_cov_3.416920, whole genome shotgun sequence.
ACCESSION   DAOQDO010000551 DAOQDO010000000
VERSION     DAOQDO010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26059_length_3010_cov_3.416920"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKZ53_09870"
     CDS             complement(<1..548)
                     /locus_tag="PKZ53_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40785.1"
                     /translation="MFAFFAATILAVFGFFGGEPVQSPQVLPAVTSGMEYKLLATNKT
                     STMQKELNQAAGNGFRYDGVMGGETAFGGSETVVIMSRNPRENLAGQYEYRLLATNKT
                     STMQKELQAAADEGYEYRGQTVFNTTFGGEEVVVILERNKETKTRRFEYKLLATQKTS
                     TMQKELENAGKDGFTFVGITVG"
     gene            778..1173
                     /locus_tag="PKZ53_09875"
     CDS             778..1173
                     /locus_tag="PKZ53_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNJ40786.1"
                     /translation="MARKKEGSQLTPLELDIMQILWDRGPSTVQEIRERLGKELAYNT
                     VQTMLNLLVKKGKADRTLKEKAYQYHSTVNQQSALRQLMDDLVHRVFGGSAENLVMSL
                     VETDHLSPEKLAHLTELVNRKRNPDDQSN"
     gene            1157..2770
                     /locus_tag="PKZ53_09880"
     CDS             1157..2770
                     /locus_tag="PKZ53_09880"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNJ40787.1"
                     /translation="MISQISLTMVSYLVHCGWIVPVLVLIGWSFERILITSRPQARFQ
                     VWAGLIILSTGLPLAFVQFPVVQPNQVTITTPVAGVVTPRTVMPPSALAADDLKLKPI
                     HQSKTVELPQLVTFGLAGGYAVLVIWWLGRILVGLYQVRQFRKTLTPVELTDSLCQRL
                     HHCAEQINVPTPLVFSGPAETSPFTFGIWRPVVVIPQTILESATPELLESVFAHELAH
                     IRRRDFGVNLVLEFCTAFCGFHPLVWFLRKRLNLYREAACDEMVTEHLIDPLTFAQSL
                     IVVADTLSNRHALTPGMFHSNTLEERIMSLTHSVDQTGNLKVSLFQRLSFGLVLSCGI
                     GAFGLIPTLIPTLAETQSPQSPQGEPIILRQRSPAGVLKRTNTVDPDAEEAFHKKAKN
                     YLQNEYEQEKAKKAPQLYFSYQLRRIDSTRAQVVCLMAAQSDSNWVEVKVEPGKMVIG
                     PDGKMVIGENGLGLVNPDGSDGMIRSGSDSPEPESIITPVFPSITFAVQPDTDAVIIS
                     FKLKKDEEPTKLIMRLDDRETTGGAIIAR"
BASE COUNT          755 a          742 c          719 g          794 t
ORIGIN      
        1 tttccaacgg tgatgccaac aaaagtgaat ccgtcttttc cggcattttc gagttctttt
       61 tgcatggtcg aagttttttg ggtggcgagc aatttgtact caaaccgcct ggttttggtt
      121 tctttgttac gttccagaat caccacgact tcttcgccgc caaacgtggt gttaaaaacg
      181 gtttggccgc gatattcata tccttcatcc gctgcggctt gaagttcttt ttgcatggtc
      241 gaggtcttgt tggtggccag gagccgatat tcatattgac ccgccaggtt ttctctgggg
      301 ttgcgcgaca taatcaccac ggtttcagac ccgccaaacg cggtttcccc gcccataacg
      361 ccatcatagc gaaacccgtt gcctgctgcc tgattgagtt ctttttgcat ggttgaggtt
      421 ttgttggtgg caagtaattt gtactccatg cctgaggtta cggctggaag cacctggggt
      481 gattgaacgg gttcaccgcc gaaaaatccg aaaacagcca ggattgtggc tgcaaaaaaa
      541 gcaaacataa gaatctctcc gaagatgttg agtggatgaa taaattgaga caggcgaaac
      601 aaagtgttga ctcagttttg gctggtcagg ttgccacaac ggtttctgac gggcgactgt
      661 tccggtgaaa cgactgaagg ccggttttta aacctgccgg aagctacctc ctaaaaaatt
      721 aggttgacaa aaacaaggct tggaagtact ctcctaaaaa attaggagag tgtcatcatg
      781 gctcgcaaaa aagaaggctc acaactcact ccgcttgaac tagacatcat gcaaattcta
      841 tgggatcggg gcccgtccac ggttcaggaa atccgcgaac gactcgggaa agagctggct
      901 tacaacacgg ttcaaaccat gctcaacctg ctggtgaaaa aagggaaagc ggaccgaacc
      961 ttaaaggaaa aagcctatca gtaccattca acggtcaatc aacagagcgc gcttcggcaa
     1021 ctgatggatg acctggttca tcgcgttttc ggaggctctg ccgaaaatct ggtcatgagc
     1081 ctggtggaaa ctgatcacct gtctcctgag aaactggctc acctcactga actggtaaat
     1141 cgaaagagga accctgatga tcagtcaaat tagccttacg atggtatcct acctcgtcca
     1201 ttgtggctgg atcgtgcctg ttctggtcct gattggatgg tcatttgaac gaattctcat
     1261 caccagtcgt ccccaggccc ggtttcaagt ctgggccgga ctcatcatcc tttcaactgg
     1321 gctcccgctg gcgtttgttc agtttccagt tgttcagccc aatcaggtga ctataacaac
     1381 cccggttgcc ggcgtggtaa cgccacgaac ggtgatgccg ccctcggccc tggccgctga
     1441 tgatctcaag ctcaaaccca ttcaccaatc caaaactgta gaactgccgc aactggttac
     1501 ctttgggctg gcagggggct atgctgtgct tgtgatctgg tggctgggtc gaatcctggt
     1561 cgggttgtat caggtccgtc agtttcgaaa gaccttaacc ccagttgaac tgactgactc
     1621 actgtgtcaa cggttgcatc attgtgctga acaaataaat gtcccgaccc cgctggtttt
     1681 ttccggcccg gctgagacaa gtccctttac ctttggaatc tggcgtccag tggttgtgat
     1741 tccacaaacc attttggaat cagccacccc ggaacttttg gaatcagtat ttgctcacga
     1801 actggcgcac attcggcgac gcgactttgg ggtgaatctg gtgctggaat tctgcaccgc
     1861 tttttgtggc tttcaccctc tcgtttggtt tctccggaaa cgactcaatc tctatcgaga
     1921 agcggcctgt gacgagatgg ttactgagca tctgattgat ccactgacct ttgcccaatc
     1981 actgattgtg gtggctgata cgctatcgaa ccgccacgca ctcacccctg gcatgtttca
     2041 ctcaaatact ttggaggaac gcattatgtc gctgactcac tcggttgacc aaaccgggaa
     2101 cctgaaagtc tccttatttc aacgcctttc atttgggctg gttttaagtt gtggaattgg
     2161 tgcctttggc ctgattccga ctttgattcc aacactggca gaaacccaat ctccccaatc
     2221 acctcaaggc gaaccgatta tccttcgaca acgatctcct gccggagtct taaaacgaac
     2281 aaatacagtt gatccagatg ctgaagaagc cttccacaaa aaagcaaaga actacttaca
     2341 aaatgagtat gagcaagaga aagccaaaaa agcaccacaa ttgtatttta gctatcagct
     2401 tcgtcgaatt gacagtacac gggctcaggt ggtttgtctg atggccgctc aatctgacag
     2461 caactgggtt gaggtcaaag tcgaacctgg aaaaatggta attgggccag atggaaaaat
     2521 ggtaatcgga gagaatgggc ttggactcgt aaatcctgat ggatctgatg gaatgattcg
     2581 ttctggatct gattcacccg aacctgagtc aattatcacc cctgtctttc caagtattac
     2641 ctttgctgtt caacccgata cagatgctgt catcatcagt ttcaaactca agaaagatga
     2701 agaaccaacc aaactcatta tgcgcctgga tgacagggaa acaactggcg gagcaattat
     2761 tgcccggtaa ggttgtttct caattacctg gctccaggaa attttgtcca cgcgtggaca
     2821 aaccacctaa gtgttgaaat gatttgagat cattcaacat tcatggtggt tttttgtatg
     2881 tcggtattca agtttccgct atcgtaaaca gtcagtcttc agtcaaatag acattattgg
     2941 gattaaccat tttccaaaag tggagcttcc tcgccgtggc aacccacccg ctcacgcagg
     3001 tggtactgac
//
LOCUS       DAOQDO010000552         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26081_length_3009_cov_2.952945, whole genome shotgun sequence.
ACCESSION   DAOQDO010000552 DAOQDO010000000
VERSION     DAOQDO010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26081_length_3009_cov_2.952945"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKZ53_09885"
     CDS             complement(<1..222)
                     /locus_tag="PKZ53_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40788.1"
                     /translation="MASLMVVHLSGEERGKTLIFDQEAITLGPDEGCDLPLNIPAVHN
                     GDIPRRLIVAEIYRQKHNFDLFFRDPEHYS"
     gene            complement(423..1727)
                     /locus_tag="PKZ53_09890"
     CDS             complement(423..1727)
                     /locus_tag="PKZ53_09890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNJ40789.1"
                     /translation="MNIAIVGTGYVGLVTGACFAEFGVKVTCVDIDEAKIAGLNEGQI
                     PIYEPGLHELIEKNLRAGRIQFTTNLRAAVERALVVFIAVGTPPKSDGSPDLSQVEQV
                     ALQIAPALNGYKVIVTKSTVPTGTGQFIRRVIEEHASGEHRFSVASNPEFLREGDAIN
                     DFLRPERVVVGCQDSEAIAILQDIYSPLTQVGVPMVVTSLETAELIKYASNAFLATKI
                     SFINEMANLCDVLGCDVQDVARGMGLDSRIGNKFLYPGPGFGGSCFPKDTRALVHLAN
                     SAGYDLKIVKSVVEVNERQHEVALDKVLELLGREVDGKVAGILGLTFKPETSDLRESP
                     AMWVASELIKLGVQLRATDPVANQEARTCLTQAEFFEDPYETAAGCDVLIFATEWNIY
                     RKLELDRLKEAMRTPAIADLRNMYDARLVRKHGFRYLGIGRR"
     gene            complement(1797..2360)
                     /locus_tag="PKZ53_09895"
     CDS             complement(1797..2360)
                     /locus_tag="PKZ53_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HNJ40790.1"
                     /translation="MENNMYADQFETNGHCLIGDETVTVQAETGVHRVLSFSVLFAEQ
                     MTLHRTRHFQAGGRTDVIYKPYLYALAIDMAEQQAVWKPVRYLFRRPYEGKLIYVVTN
                     DNRRITVTDQHPMIVFEGDQLVVRPAASLKQGDRVPLYWQGTVATIPIKGLVTCTAST
                     TVYSMEVDDTHTFATSYGIYVHNCIPL"
     gene            2541..2960
                     /locus_tag="PKZ53_09900"
     CDS             2541..2960
                     /locus_tag="PKZ53_09900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNJ40791.1"
                     /translation="MANRLTLEIVTPDRLLLHKEADEITVPGLNGEMGILPGHTPLIS
                     QLSVAGLVTYRNGSEQGLAFVSQGFAEVMPDRVTILAERAQLPEEIDLAKAKAALQDA
                     ERVLKSAEKDASINIESALAALDSAIVQVQVAERTKR"
BASE COUNT          816 a          780 c          692 g          721 t
ORIGIN      
        1 ggagtaatgc tcggggtcac ggaagaagag gtcgaaattg tgtttttgcc gataaatctc
       61 agccacaatc agacgtctcg ggatgtcccc attgtgaacc gccggaatat tcaacggcaa
      121 atcacaacct tcgtcaggac cgagggtaat tgcttcttga tcaaaaatta acgttttacc
      181 acgttcttca ccggagaggt gaacgaccat caggcttgcc atgacggaac ttcaaaacct
      241 tgctcaaaaa caactttgaa taactgcgtg aaaaaattgg atgaaggaaa tcagataccg
      301 caacgacaca ctatactgat tcactccaaa catcacaagg gaaagatcac ctggaaattt
      361 tagaatgaag aatgaagaat ctcaccttgt caccctcgca ccctgtcatc ctgtcactga
      421 tcttagcgtc ttccaatccc caaataccgg aagccatgct ttcgaaccag cctggcgtca
      481 tacatgttgc gcagatcagc aatggccgga gtccgcatgg cttctttcaa acgatcaagc
      541 tccagtttgc ggtaaatgtt ccactcggtg gcaaagatca acacatcaca tccagccgct
      601 gtctcatagg ggtcttcaaa aaactcagcc tgggtaaggc aggttcgcgc ttcttgattg
      661 gcaaccggat cagttgcccg gagttggact ccaagcttga tcagttctga agcaacccac
      721 atcgcggggg attcgcgcaa atcactggtt tcaggtttga aggtcaatcc aagaatgccc
      781 gccacttttc catcaacctc ccggccaagc aattccaata ctttgtcgag tgccacctcg
      841 tgttgccgct cattgacttc aaccactgat ttgacgattt tcaagtcata cccagccgag
      901 ttggcaaggt ggaccagtgc ccgggtatct tttggaaaac aggagccacc aaatcccggc
      961 cctgggtaca aaaacttgtt gccaatgcga ctgtcaagcc ccatcccacg tgccacatcc
     1021 tgtacatcgc atcccaacac atcgcacaaa tttgccatct cattgataaa tgaaatcttg
     1081 gtggcgagaa atgcgttcga ggcatatttg atcaactcag ccgtttccag cgaagtcacc
     1141 accattggca cacccacttg ggtcaatgga ctgtaaatat cttgcaggat ggcaattgct
     1201 tctgaatcct gacatccaac aacaacccgc tctgggcgta aaaagtcatt gatggcatct
     1261 ccctcgcgta aaaactccgg gttcgaagcc acactgaagc gatgctcacc tgaggcatgt
     1321 tcctcgatta cccgccggat aaactgacca gttccggttg ggaccgtgct tttggtcaca
     1381 atgactttgt acccgttgag cgcgggtgca atctggagcg ccacctgctc aacctgtgat
     1441 aaatcaggac ttccatctga tttgggtggt gttccaaccg caataaacac caccagcgct
     1501 cgttcgacag ctgcacgcag attggtagta aattgaatcc ggccagcacg tagatttttc
     1561 tcaatcaatt cgtggagtcc tggctcataa attggaatct ggccttcatt caagccagca
     1621 attttcgcct catcaatgtc cacacaggtg accttgacgc caaattcagc aaaacatgcg
     1681 ccggttacca aaccaacata gcctgttcca acaattgcaa tattcataag tcgcgaagaa
     1741 agtccaaatt tggggtacag cggcttgatg ccgcgttgat cctgggccag ccagttttac
     1801 agcggaatac agttatgaac atatattcca taggaagttg caaatgtatg agtatcatcc
     1861 acttccatcg aatagactgt cgttgacgct gtacaggtta ccaacccttt gatggggatt
     1921 gtggccacag tcccctgcca gtacaatggg acacgatctc cctgtttcaa gctggcagcc
     1981 gggcggacga caagctggtc accttcaaaa acaatcatgg ggtgctgatc ggtcacggtt
     2041 atccggcgat tgtcattggt gaccacatag atcaatttcc cctcatatgg acgacggaac
     2101 aagtaccgaa ctggcttcca gacggcttgt tgttcagcca tatcaattgc cagtgcatac
     2161 agatagggct tgtaaatgac atcagttcga ccaccagctt gaaaatgcct ggttcgatgc
     2221 aacgtcattt gttcagcaaa caacaccgaa aacgacaaca ctcgatgaac accggtttcg
     2281 gcctgaaccg ttacggtttc atcgccaatc aggcaatgac cgttggtctc aaactggtct
     2341 gcatacatgt tgttttccat aggggagtga ctgattgtgg aatggttggt tgggcaaaga
     2401 agggcgatac tgacaaacca cggaagcgat gtcaacagag gatccagtgt gaaatggctt
     2461 gaaccaaaaa gcgttgactg gtatcttggc gggtcatttt ccaaccttaa tttatttcta
     2521 ttctttcagg aatcatactt atggccaatc gccttacttt ggaaattgtt acccccgacc
     2581 gattactgct ccataaagaa gcggatgaaa tcaccgttcc cggcctgaat ggggaaatgg
     2641 gcatcctgcc tgggcatacc cccttgatct cccaattgag tgtggccgga ctggtcacct
     2701 accgcaacgg aagcgaacaa gggctggctt ttgtcagcca gggatttgct gaagtaatgc
     2761 ccgaccgtgt tacgattttg gcagaacgag cgcaactccc tgaagaaatt gatcttgcca
     2821 aggcgaaagc ggcactccag gatgctgaac gtgtactcaa gagcgccgaa aaagatgcca
     2881 gtatcaatat tgaaagtgcc ctcgccgcac tcgacagtgc cattgtccag gtccaggttg
     2941 ccgaacgcac caaacgctaa ccctttgaga atgaagaatt gagaatgaag aattgagaat
     3001 gaagaattg
//
LOCUS       DAOQDO010000553         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26117_length_3007_cov_2.914973, whole genome shotgun sequence.
ACCESSION   DAOQDO010000553 DAOQDO010000000
VERSION     DAOQDO010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26117_length_3007_cov_2.914973"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKZ53_09905"
     CDS             <1..282
                     /locus_tag="PKZ53_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40792.1"
                     /translation="DGPKGPKYEAKPGAVMLARLSGAAILPMCVTTEKYWEFKSWDRF
                     RVPKPFSRARIAYGRPIVVTRQATEIEMAAQQAELQAALDTLEIQSQKW"
     gene            complement(283..453)
                     /locus_tag="PKZ53_09910"
                     /pseudo
     CDS             complement(283..453)
                     /locus_tag="PKZ53_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
     gene            complement(809..1222)
                     /locus_tag="PKZ53_09915"
     CDS             complement(809..1222)
                     /locus_tag="PKZ53_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNJ40793.1"
                     /translation="MSEHTPRTFSIGECASLSKTITETDVTLYAGITGDFNPVHIDAE
                     YAKTTRFGARIAHGMLTTGFISAVLGMKLPGPGAIYLGQELKFLKPVYIGDTITARVE
                     VLEFHAEKKILVAKTECLNHRGETVLTGTAKLLVP"
     gene            1552..1842
                     /locus_tag="PKZ53_09920"
     CDS             1552..1842
                     /locus_tag="PKZ53_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40794.1"
                     /translation="MELSEDLEALYRQLALKKYKGTLTDDEALIFADLTDQRNKVDTS
                     SAALGSLPTSMSKFMSDLGQFEGTMDDMSRKIQNKESYLDLAQRKPDSSEDR"
     gene            complement(1932..>3007)
                     /locus_tag="PKZ53_09925"
     CDS             complement(1932..>3007)
                     /locus_tag="PKZ53_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNJ40795.1"
                     /translation="SARRSIQIYLKDEYPPELITEQRESELVVKARHQGFFSYTLFAT
                     SRGAFTFGGIAARYLGPLGLVWRQTTFPATQTVKVYPNIQEARKNELIALRNRDLRLG
                     QRRMRHKGQGREFESLREFVTGDEVRHISWSATARRGKLVTREYQVERSQNIVVMIDC
                     GRLMTTRIGQLTKLDYAVNAALSIAYVATASGDNMGILAFCRQVTHYLPPKHGRTQLA
                     KVLDSLYDVKAELIEPSYARAFSYLNTNCRRRSLVVILTDLVDRDASAELLTHTATLF
                     PRHLPLIVTIGDKDLWGLVREVPKTSEAVFEQSVAEELLSERESALRRITQLGGLALD
                     VPTGKLSLELVNKYLEVKERGLL"
BASE COUNT          813 a          748 c          721 g          725 t
ORIGIN      
        1 gatggtccaa aagggccaaa atatgaagcc aaacctggag cggttatgct ggctcgcctg
       61 agtggagcag caattttgcc aatgtgtgtc accacggaaa agtattggga attcaaaagc
      121 tgggaccggt ttcgggtgcc aaagcccttt tcacgcgccc ggattgccta tggtcgcccg
      181 attgtcgtga cacggcaggc aactgaaata gagatggctg cccaacaagc agaactgcag
      241 gcagcccttg atacactcga aatccagagt cagaagtggt agttactcaa ccggatgcga
      301 tgccagccag ctttgaacgt cctcaaccga ggcaatttca aagagaacca gggcaaaggc
      361 ttcgagagac gaagctggta attgttcaat ttgcgaaatt agattttcag gcaaggtgcc
      421 cagtttctgg gtcagcattc gcaacaaaat aaatcgctct ccttgaacct gaccttattg
      481 caagccttgt tgcaagcctt gctgcagtcc ttcttctaaa atttcctgat aaattactga
      541 ttcctgcata acacgctcct gcaataagct tttgatgaga tttttgtcaa accgaagccc
      601 agccaggatt tgggcacaag ccagagtgtc tcgtcaggga ttgtcagtac cacccgcgtg
      661 agcaggtggg gttagaaaca aaaaggccca cccactgacg cgggtggtac agacttggca
      721 gtttcggctg attcgcctcc aacacaaaat ccggtagaag cagcaaaatt gaattgtcag
      781 ggatagacgg cctgatcctt caaatttttc atggcaccaa gagtttagca gttccggtta
      841 aaaccgtttc accacggtga ttgaggcatt cggttttagc caccaggatt tttttctcgg
      901 catgaaattc caaaacttca acgcgtgccg ttatggtgtc accaatatac accggtttga
      961 ggaacttcaa ttcctggcca aggtagatgg cccccggccc cggaagcttc atgccaagca
     1021 cggctgaaat aaacccggtg gtcagcatcc cgtgggcaat tcgagcgcca aatcgcgtcg
     1081 ttttggcata ttcggcatca atatgcaccg ggttaaagtc accagtaatg ccggcataca
     1141 gagtaacatc agtttcagtg atggtttttg acagcgaagc acattcgccg atggaaaaag
     1201 ttcgtggggt atgttcactc ataaatgtaa cttcaggaaa tcttgagagt taagctggat
     1261 ttaaagcttg cggctggaag attgcggcct ctatattaca ctagtttgta gtcagtagtc
     1321 agtggtcagt agttcactaa ctccattttg atgaattatt taactgcttt ctaatatgag
     1381 aattccattc caaagtgata taagacactt ttgcaatgaa taccttgcat tagaaaatag
     1441 ttaagtaatt caatcaaatg agcttaacaa actactgact actgactact gactactgac
     1501 tggtattatt cacaccttcc actgcaaaat cacgctcagg agcatctgat tatggaattg
     1561 tctgaagacc ttgaagccct ctatcgccaa ctcgcgctca aaaagtacaa aggcacactc
     1621 actgacgacg aagccctgat tttcgcggac ctgactgacc agcggaacaa agttgatact
     1681 tcatcagccg cgcttggctc gttaccaacc agtatgtcca aatttatgag tgatttaggg
     1741 cagtttgaag gcacaatgga cgacatgtcc cgaaaaattc aaaacaaaga aagttatttg
     1801 gatctggccc aacgcaaacc tgattcatcg gaagacaggt gaaccgggtt ccgggttgaa
     1861 aaaccagggc tgagggcttg gggctgaggg ctgaaaaatc agggttgaaa aaccaggtca
     1921 ccaggcactg gtcacaaaag ccctcgttcc ttcacttcca ggtatttgtt gaccagttcc
     1981 agcgagagtt ttccggttgg gacatcgagc gccaacccac caagctgggt aatccgacgc
     2041 agcgccgatt cccgttcgga caataattct tcagcaactg actgctcaaa cacggcttca
     2101 gaggtctttg gtacctcacg aaccagtccc cacaaatcct tatccccaat ggttacaatc
     2161 aatggcaaat gccgtggaaa cagggttgcc gtgtgggtca gaagttctgc cgaagcatca
     2221 cgatccacca gatctgtgag aatgacaact agcgaccggc gccgacaatt tgtgttgaga
     2281 taggaaaacg cacgggcata agacggttca atcaattcag ccttcacgtc atacaaacta
     2341 tccagaactt tcgccagttg tgtccgccca tgtttgggtg gcaggtagtg ggtgacctgt
     2401 cggcaaaaag ccaaaatccc catattgtca ccactggccg tggccacata cgcaattgaa
     2461 agtgccgcgt tgacggcgta gtcgagtttg gtcaactgtc caatgcgggt cgtcatcaat
     2521 cgcccgcagt caatcatcac gacaatattc tgactgcgct cgacttgata ctcccgagtc
     2581 actaactttc cacgccgggc tgtggctgac caggaaatat ggcggacttc atcaccggtg
     2641 acaaattccc gaagcgactc aaattcacgc ccctgcccct tatggcgcat tcgccgttgt
     2701 cccaaccgaa ggtcccgatt tcgaagcgca atcagttcat tctttctggc ttcctgaatg
     2761 ttgggataga ccttgacagt ttgggtcgcc gggaatgtcg tctgacgcca gaccagtccg
     2821 agcggaccaa gatagcgggc agcaatgcca ccaaaggtaa aggcaccccg cgaagtggca
     2881 aataaagtgt agctaaaaaa cccctgatgc cgcgctttga ccaccagttc actttcacgc
     2941 tgttcggtga tcaattctgg cgggtattca tctttcagat agatttggat tgaacgtctg
     3001 gctgagt
//
LOCUS       DAOQDO010000554         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26118_length_3007_cov_2.866870, whole genome shotgun sequence.
ACCESSION   DAOQDO010000554 DAOQDO010000000
VERSION     DAOQDO010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26118_length_3007_cov_2.866870"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKZ53_09930"
     CDS             complement(<1..816)
                     /locus_tag="PKZ53_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ40796.1"
                     /translation="MLSHLRPTEISRPFGIPVLLDPSWYVIFVVYLWLIGWVYLPGLV
                     PGLPMVTYLGFGLLTALLFFLSILIHELAHSLVARAEGVGIHNITLYVFGGLAHLTGE
                     PASPGAEFRIAAAGPAASCVLGVMFLVAEWLLQQIAHRAVATQICHYLGWLNVILAGF
                     NLIPGFPLDGGRIVRAGLWWRQGSFHQATLQVMRFSLWIANVLMVVGGALFFLRWNLG
                     LSIVVTGAFMRILTLRSLPRSPESSVKPVSPPARCGPIAQDAIATQLVSVTPDT"
     gene            complement(1059..2198)
                     /locus_tag="PKZ53_09935"
     CDS             complement(1059..2198)
                     /locus_tag="PKZ53_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40797.1"
                     /translation="MDNLIWANVQHRPTRSLATALGVAVSTVLILLTVGLARGVLHER
                     STREAKVGAEIQVRSSGTMTAGIPTNQPSLPIARAEEIRKIAGVKIVSPIIQYVQASD
                     SGLGFRAIEAVNFEEYAQISGIRLLEGNPPQTDEEVIIDDHLVKERKVKTGQEMEVMG
                     RMMKISGVYGPESGARIKMRLSTMQERLAAQNLCSMLLVKCESPAAQDAVAIQIQKNS
                     DERVRQDKAGTEVADQVILTRDLPNFYAKGLPALDTFLNVVVGVAMGISVLVTLLAMY
                     TTVSERTREIGILKSLGASNSFILRVIEQEALLISAAGALLGYVLAWSARFGITHATA
                     IKNIEFEPHWILITFVIALLGGAIGALYPAWRAVRLDPVKALSYE"
     gene            2625..>3007
                     /locus_tag="PKZ53_09940"
     CDS             2625..>3007
                     /locus_tag="PKZ53_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40798.1"
                     /translation="MKTPPNYDVLLIRAVFVVIFVATAWFLRPFSGNALLSAVIALSS
                     SLVIIFFEFRIRRASFKALIGGTLGIILGFLCATLVGSLLSIQDGIAGQSPALKSFVT
                     LSVVFSFGYIGLLIGATKFDFRDLIS"
BASE COUNT          761 a          809 c          720 g          717 t
ORIGIN      
        1 agtgtcaggg gtcactgaaa ccagttgggt tgcaatagca tcctgggcga ttgggccaca
       61 gcgggcgggt gggctgactg gcttcactga agattctgga gaccgaggca atgatctcag
      121 cgttaagatc cgcataaacg ccccagtcac tacaatcgaa agccccaaat tccaccgcag
      181 aaaaaataag gcaccaccca ccaccatcag cacattggca atccataagc tgaagcgcat
      241 gacctgaagc gttgcctggt gaaaactacc ctgcctccac cacaaaccag ctcgcacaat
      301 ccgcccacca tccagaggaa acccaggaat caggttaaac ccggccagaa tcacattcag
      361 ccagcccagg taatggcaaa tttgagtggc aacggcacgg tgagcaatct gttgcaacaa
      421 ccactcagcc accagaaaca taacgccaag cacacaactt gccgccggac cggctgccgc
      481 aattcgaaat tcagcgcctg gactggctgg ctctccggtt aaatgggcca ggccgccaaa
      541 cacatagagc gtaatattgt gaattccgac accttcggcc cgggccacaa gcgaatgcgc
      601 cagttcatga atcaaaattg acaggaaaaa taacagtgcc gtgaggaggc caaacccaag
      661 gtaggttacc atcggaagcc caggaactaa cccaggcaaa tacacccacc caatcagcca
      721 taaatacacc acaaaaatga cataccagct tgggtcaagc agcactggta ttccaaacgg
      781 gcgactaatt tcggtcggac gcagatgtga aagcattgaa aaaccagatt tgaagacatc
      841 gggttgaaga atatcgggct gaagacattg ggcttggggc tgaagaatat cgggctgaag
      901 aatatcgggc tgagggcttg gggctgaaga agtcgggctg aaagaaccag ggctgagggc
      961 ctggggctga agaagtcggg ctcaggtatg aaacccgttt tattcagccc cgagctggcg
     1021 agtcttcagc cctcagcccc cagcccctgg ttttccgcct attcatatga cagtgctttc
     1081 accgggtcga ggcgaactgc acgccaggcc gggtacagcg ccccaatcgc accacccagc
     1141 aacgcaatga caaacgtaat caaaatccag tggggttcaa attcaatatt tttgattgcc
     1201 gtggcgtggg taatgccaaa ccgggcactc catgccagaa catagccaag caaagcacct
     1261 gccgcactaa tcaacagcgc ttcctgctca atcacacgca aaataaaact gtttgaggca
     1321 ccgagtgatt ttaaaatccc aatctcgcgg gttcgttccg agaccgtggt atacatcgcc
     1381 agcaaggtta ccaggacact gatgcccatg gccaccccga ccaccacatt gagaaaggta
     1441 tccagggcgg gcaggccctt ggcataaaaa ttggggagat cccgagtcaa aataacctga
     1501 tccgccactt cagttccggc tttatcctgg cgaacgcgct catccgagtt tttctggatt
     1561 tgaatcgcta cggcatcctg ggcggctggt gactcacatt tgaccagcaa catcgagcac
     1621 aggttctgag ccgccagtcg ttcctgcatc gttgaaagcc gcattttgat ccgggcaccg
     1681 ctttcaggtc catagactcc ggaaattttc atcatgcggc ccatgacttc catttcctga
     1741 cctgttttta ccttgcgctc tttgaccagg tgatcatcaa taatgacttc ttcatctgtc
     1801 tgaggtgggt tgccttcaag taaccggata ccgctgattt gtgcgtattc ttcaaagttg
     1861 acagcttcaa ttgcccgaaa gcccagacca gaatccgacg cctgaacata ttgaataatc
     1921 ggggaaacaa tttttacccc ggcaatcttt ctgatttctt cagcccgggc aatggggagc
     1981 gaaggctgat tggtcggaat accagcggtc atcgtcccac ttgaccgaac ctggatttca
     2041 gcacccactt ttgcttcccg cgtgcttcgc tcgtgaagaa ccccccgagc taatccgact
     2101 gttaataaaa tcaacaccgt gctcacggca accccaaggg ccgtggccaa agaacgggtg
     2161 gggcggtgct gaacattggc ccagattaaa ttatccatga cgtactggta ctctcaatca
     2221 acccactctg agacaaagtg ggtctggttt tcaactttct ttaaaatctt tggatgtagg
     2281 agaaaagggg tcaatgtaca cgttgggcac ggtttgagca atcataagtt gacaaccagc
     2341 ggcatgttgt tgctcaaaga tcttgcaagc acacggtgaa tcgttcaaag taaggcagca
     2401 ttaattttca ttttccaaat aagatcatac ttatatcgag gttgtctggc tcgtttcatt
     2461 ccccctggaa cttcttccga ttcaacttgt cttgcatcca catccaagcc aagcctgata
     2521 ttttccatgc gctcatcgtg cgtggcgcct ttcctttttt cgaagcggag gattttgaac
     2581 caacgacctg tcgttacaga ggactcgcac ttgtgaaggg ctttttgaaa actccaccga
     2641 actatgatgt gttgctgatc cgggcagttt ttgtcgtgat ttttgtggct accgcatggt
     2701 ttcttcgtcc tttttctggg aatgcactgc tctctgccgt cattgccttg tcatcatcgc
     2761 tggttatcat cttctttgag tttcggatcc gtcgcgcttc attcaaagca ctcattggcg
     2821 gaacgctggg tatcatcctt ggttttttat gcgccacgct ggttgggtcg ctgctttcca
     2881 ttcaagatgg tattgccggc cagtcaccag cactcaaatc ttttgtgacg ctgagcgtcg
     2941 tttttagctt tggctatatc ggcttattga tcggcgcaac caaatttgat tttcgtgatt
     3001 tgatctc
//
LOCUS       DAOQDO010000555         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26152_length_3005_cov_2.858983, whole genome shotgun sequence.
ACCESSION   DAOQDO010000555 DAOQDO010000000
VERSION     DAOQDO010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26152_length_3005_cov_2.858983"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKZ53_09945"
     CDS             complement(<1..344)
                     /locus_tag="PKZ53_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40799.1"
                     /translation="MISVLISDLLDQIQGKPTSRQVSPEGTAVPSDSPILRAITQAGL
                     LLERYTFAIENEGFYQAMLTPDLLHHSLTEEESIALITALSELLKTDPTHAGMAAWAL
                     GRTSEDGAREALA"
     gene            935..>3005
                     /locus_tag="PKZ53_09950"
     CDS             935..>3005
                     /locus_tag="PKZ53_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNJ40800.1"
                     /translation="MKSQLRSDFARRVFNPLFMSCLILCFGLYLGGNAATGLVQAWQE
                     GRQSRASQLDAPAVPTEALPAPPQTDQEVASLTVNPQAVFTVSNTNDSGSGSLRQAIT
                     SALGAGAGPHFIEAGGVTGTISLQSALPTITNSTITINGPTSGTLTVTRGVAANFRIF
                     TVTNASGAASLTINRLTMTNGNPGTGTSDNGGAISVTDSALTLNFCTISGCSAGGQGG
                     GVNAEGTTGATLVINFCTISGNTAASSLGGGVCVRMNSVAPSPISTLLMTNSTVSGNT
                     VTGGGAGGVNLTYTAGTFRNCTISGNSSGAGNAGGISVASVSSLAMTNCTMTNNRCAA
                     GSLGGGIRSLSGNSVTLTNTLVVGNFNGPTGTTADDTSWTNGTGTFTAVNSVIGADGG
                     GGFDSSTASQVGTTASPKVVNLGALTNNGGPTQTHALLATVPELAINLGDNTSASSLA
                     SDQRGIGFNRFNGIVDIGAFENGSTSPSFTGVTALGAPAQVTGVTSLTYTVPPGTNRL
                     LVVTASNATSTTIGSVSFGASVMTMAVQKNDGFAMDSIWSLALGTSTSSDTRTITVTG
                     FSTAVNTVLTAQTFQNVDQTTPIDGTLNNAATGTSVGSTLTLLTTTANDLVFDLFDCF
                     VNSGAITATPGAGQAMVHGANLSIVSPSGGIGRYVTSIKPAVGSSTTMSWASNTLAII
                     QLAANINY"
BASE COUNT          668 a          807 c          776 g          754 t
ORIGIN      
        1 gccagtgcct cacgggcgcc gtcttcgctg gtccgaccaa gggcccaggc agccattccg
       61 gcgtgggtcg ggtcagtttt caagagttcg ctgagagcgg taattaaggc aatggattct
      121 tcttcggtca gtgaatggtg cagcaagtca ggcgtcagca tggcttgata gaagccttcg
      181 ttttcaattg caaatgtgta gcgctccagc agcagcccgg cttgagtgat ggctcgcaga
      241 atgggcgaat cactcggaac ggcagtgcct tctggcgata cctggcgact ggttggtttt
      301 ccctggattt gatcgaggag atcagaaata agaacgctga tcattccgaa tgaatccttt
      361 caatggacaa agttgaccgg gatttgccga agaatctggt ctgaattcga attccttcct
      421 ggtcactctg tagcatacct tgaatgtgtt tgtgatgggg attatttcac cgcattccag
      481 gctaccttgt ccaccagatt tcggttgggg aacagtgacc tcccgctggt atcgaattca
      541 gtagtgataa agaatgacct cacccataag ccaacaatgt gacaggtcta ttttatgaca
      601 ccaggccggg ttgttgttac tttccttgac ggattgtttt tgagagagta caacttgtca
      661 tcggctccaa acctgaaaaa tcttttccct atctgtactt ttcatattgg gttaccaggc
      721 tgccaggaat gaaaattaca gtttttgggc gattttccct tcttcaataa cgggtgtcat
      781 cggggtttta ttgtctacag gcaataaaag gactgaggtc cgtctgttgt ctacctcctg
      841 gtgatgctgg ctgattgtcc cctgtatcga attctctatc gttgaagcca atcacatctc
      901 ctgacttttt ctgacttttt caaggtgcat atctatgaaa tcccagcttc gatctgattt
      961 tgcccgtcgt gtgttcaacc ctctttttat gagttgtctc atcttatgtt ttggcttgta
     1021 tctgggtggt aatgccgcaa ctggactcgt tcaggcctgg caagaaggcc ggcaatcccg
     1081 tgcatctcaa ctggatgcgc ccgccgtgcc gactgaggct ttacccgcgc ccccacaaac
     1141 agatcaagag gttgccagcc tcaccgtcaa tccacaggct gtatttaccg tttccaatac
     1201 caacgatagc ggatcaggat cactgagaca ggccatcacg agtgcactcg gggcaggtgc
     1261 tggaccacac ttcatcgaag ccgggggcgt tactggcacg atcagcctgc aatcagcgct
     1321 ccccacaatc accaatagca ccattaccat caatgggcca acctccggga cattgacggt
     1381 gacacgcggg gtggcggcaa actttcgaat tttcaccgtc accaatgcct cgggcgccgc
     1441 ttcactcacc atcaaccggt tgacaatgac caacggcaat ccgggaactg gaacctcgga
     1501 caacggaggg gccatcagcg tcactgattc tgcgttgacg ctcaactttt gcaccatttc
     1561 cggctgtagt gctggcggcc agggcggagg ggtcaatgct gaaggcacga ctggggctac
     1621 gctggtgatc aacttttgta ccatctcagg aaacacggca gcctctagtt tgggtggtgg
     1681 cgtctgtgtc cgtatgaatt ctgtggcacc gtcccccatc tcgacactgt tgatgacaaa
     1741 cagtaccgtc tccggcaaca ctgtaactgg cggcggcgcc gggggcgtga acctgacata
     1801 tacggcaggc acgtttcgta actgcaccat ctctggcaat agttctgggg ccggtaatgc
     1861 cggcggcatt tcagttgctt cagtcagctc gctggccatg accaactgca ccatgaccaa
     1921 caatcggtgt gcggctggct ccctcggtgg cggtattcgc agcctgtctg gtaactcagt
     1981 caccctcacc aatacactgg tggttggaaa ctttaatggt ccaactggta ccacggcgga
     2041 tgatacaagc tggaccaatg gcaccggtac gtttacggct gtaaactctg tgattggcgc
     2101 cgatggcggt ggcggttttg attcaagcac ggcttcacaa gtcggcacga ccgcctcacc
     2161 caaggtggtc aacctcggtg cgcttaccaa caacggtggt ccaacacaaa cccacgcgct
     2221 gcttgccacg gtgccggaac tggccatcaa tctgggcgat aacacatctg cctcatctct
     2281 cgccagtgac cagcgcggca ttgggttcaa ccggtttaac ggtatcgtag acattggcgc
     2341 ttttgagaat ggctcaacct caccttcatt tactggcgta actgctttgg gggcgccggc
     2401 gcaagtgaca ggcgtgacaa gcctgaccta cactgttccg ccggggacaa atcggttgct
     2461 ggtggtaaca gcttcaaatg caaccagtac cacgattggc tcagtgagtt ttggggcgtc
     2521 agttatgacc atggctgttc aaaaaaacga tggatttgca atggattcca tctggtcgct
     2581 ggcacttggc accagtacct caagcgacac ccggacgatc accgtgactg gtttttcaac
     2641 tgccgtcaat acggtcctta ccgcacaaac atttcaaaat gtagaccaga caacaccaat
     2701 tgacggtacc ttaaacaatg ccgcaaccgg gaccagtgtt ggttccacgc tgacgctcct
     2761 gaccaccacc gctaatgatc tggtttttga tttatttgac tgttttgtca atagtggtgc
     2821 cataactgca acccctgggg ctgggcaggc gatggttcat ggagccaatc tttccatcgt
     2881 ttcaccatcc ggggggattg gtcgctatgt gaccagcatc aaaccggcag tgggatcttc
     2941 gaccacaatg agttgggctt caaacaccct cgccattatt cagttggcag caaatatcaa
     3001 ctatg
//
LOCUS       DAOQDO010000556         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26183_length_3003_cov_3.283582, whole genome shotgun sequence.
ACCESSION   DAOQDO010000556 DAOQDO010000000
VERSION     DAOQDO010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26183_length_3003_cov_3.283582"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..>3003)
                     /locus_tag="PKZ53_09955"
     CDS             complement(68..>3003)
                     /locus_tag="PKZ53_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ40801.1"
                     /translation="PRPATDQDRAKIDLDRLTIQITDLQNKEKLDEALPLALRVVEIA
                     ERIFGPEDLALADKLHDLALLVQEKGEYAKAEPFFVRAIAIQEKALGPDHPSVATTLN
                     NLARLYQDTGDYTRAEALYLRSLAIKEKALGPDHPSVVTRLENLAGLYQTQGEYDKAE
                     PLFQRVLAIAEKTTGPDDPSVGSALSELASLYCDKGDYARAEVLYLRALAIKEKALGP
                     DDPSVGKSLNDLALLYYEKGDYANAKPLYLRALAIDEKTSGLNASSAATTMNNLAIVY
                     MDEGDLAKAESLLRQALVIQEKVFGSEHPATARTINNLAWVYYNRADYARAEALFKQS
                     LAIKEKTVGPDHPSVATRLNNLGRIYQLQGDYTRAEPLYLRALAIWEKKLGPDHPSVA
                     NVLNHLAKLYWAKGELPQAIRYQIQTNEVVERDLCRNLVTGSENQKGLYLEQSGYYTD
                     QTISLHTQGAPQSLDALQTAFTVVLRRKGRELDAMASGIESLRRRATPEDQALLNELT
                     LVKKQLSNLTLKGPEKDTLEVYQARVKALETQGETIEAKISARSIEYRVQTQPITLEA
                     IQKLIPANAALVEFAIYQPYHVKTQVSGAPHYVVYLMTGSGSVNWVDLGEAEPIERVV
                     QDFRQLLANPEASPRTRKLSLGTQGVESNSLNRTAQKLDQLVMKPVRELIGKSTHLLI
                     SPDGALNLIPFSALRDETNHYLVEKYTVTYLTSGRDLLRLQAKIASQDPPLVLADPNY
                     MQGPGPNLFGQSFQPLSRLKGTHQEGNQLKAIFPEARLKLETEATEAVLKSAIKPQIL
                     HIATHGYFLQNAPQEKLTEATRLLEREDEMPSGNAEKIKEKNPLLRSWLFFAGANQGG
                     KEDNDGIMTALEASQLDLWGTKLVVLSACDTGVGDVKNGEGVFGLRRALVLAGSESQM
                     MSLWSVSDKATRELMVEFYTRLKAGEGRSEALRHVQLRMLRDQKWRHPFYWASFIQSG
                     DWGKL"
BASE COUNT          620 a          821 c          791 g          771 t
ORIGIN      
        1 agcccgatgt cttcagcccc cagcccgatg tcttcagccc ccagcccgat gtcttcagcc
       61 cccagcccta taatttgccc cagtcccccg actggataaa actggcccaa taaaacggat
      121 gccgccattt ctggtccctg agcatgcgca gttgtacgtg ccggagcgct tcagagcggc
      181 cttctccggc tttgagccgg gtgtaaaact caaccatcaa ctcccgagtg gctttatctg
      241 acactgacca gagactcatc atctggcttt ccgagccggc caggaccagg gcccggcgca
      301 atccaaacac gccttcacca tttttgacat cgcccacacc agtatcacag gctgaaagca
      361 ccaccagttt ggtgccccac aagtcgagtt gtgacgcttc aagcgcggtc atgataccgt
      421 cgttatcttc tttcccgcct tgattggcac ccgcaaagaa aagccaggag cgtaacaacg
      481 gatttttttc tttgattttc tccgcattgc cagatggcat ttcatcttcc cgctcaagca
      541 ggcgggtggc ctcagtcagt ttttcctgag gcgcgttttg caaaaaataa ccgtgggttg
      601 caatgtgaag aatctgaggt ttgatcgcag atttgagtac cgcttcagtg gcttcagttt
      661 ccagctttaa ccgggcttcg ggaaagatcg ctttgagttg atttccttcc tgatgagttc
      721 ctttcaggcg ggaaaggggt tggaacgatt gtccaaacag atttggtccg ggaccctgca
      781 tataattggg gtcagccaga accagcggcg gatcctgact ggctattttg gcctgcaatc
      841 ggagcaaatc gcggccactg gtgaggtagg tcaccgtgta cttttcaacc agatagtgat
      901 tggtttcatc cctcagggca gaaaatggaa tcaaattgag cgctccgtcc ggtgagatga
      961 gcaaatgggt agattttcca atcagttcac gcactggttt catcaccaac tgatccagct
     1021 tttgggcagt ccggttcaat gaatttgact caaccccttg tgttcccagt gacagcttgc
     1081 gagtgcgggg tgaggcttcc ggatttgcca gcaactgtct gaagtcctgg acaacgcgct
     1141 caatcggttc agcttcgccc aaatcaaccc agttgacact tccgctcccg gtcatcaggt
     1201 acacaacata gtgcggtgca cctgagacct gtgttttcac atggtacggt tggtaaatcg
     1261 caaactcgac cagtgctgca ttggccggga tcagtttctg aatggcttcg agggtaatgg
     1321 gctgggtctg gactcgatat tcaatactcc gggcactgat tttggcttcg atggtctctc
     1381 cctgggtttc cagtgctttg accctggcct gatacacctc aagcgtatct ttttccggtc
     1441 ccttgagagt caggtttgaa agttgttttt ttaccagggt cagttcattg agcaatgcct
     1501 gatcttcagg tgtggcccgg cgcctgagac tttcaatccc agacgccatg gcatccagtt
     1561 cccggccttt gcgacgcaaa acaactgtga acgccgtttg cagggcgtct aaactttggg
     1621 gcgcaccctg agtgtgtaat gaaatggttt gatcggtgta gtatcccgat tgctccagat
     1681 acagcccttt ttgattttca gaaccagtca ccagattgcg acacaggtca cgttcgacca
     1741 cttcgttggt ctgtatctga tagcggattg cctgggggag ctcacctttc gcccagtata
     1801 atttggccag atggtttaag acattggcca cggatggatg gtcaggccca agctttttct
     1861 cccagatggc cagtgcccgc agatacagcg gctcggcccg tgtatagtct ccctggagct
     1921 ggtaaatccg acccaggttg ttgagccggg tggcaacgga tggatggtca ggaccaactg
     1981 tcttctcctt gatggccagc gactgtttaa aaagggcttc ggcccgtgcg tagtccgccc
     2041 gattatagta gacccaggcc agattgttga tcgtgcgggc cgtcgctgga tgttcagagc
     2101 caaacacttt ctcctggatg accagtgcct gccgtaacag cgattcagct ttcgccaggt
     2161 cgccttcgtc catatagaca atggccaggt tattcatggt cgtggctgcc gacgatgcgt
     2221 tcagaccaga tgttttttca tcaatggcca aagcccgcag gtagagcggc tttgcatttg
     2281 cgtaatcgcc cttctcatag tagagcaacg ccagatcatt caggctcttg ccaaccgatg
     2341 gatcatcagg acccagtgct ttttctttga tggccagagc gcgcaggtaa agcacttctg
     2401 cccgtgcata gtcacctttg tcacagtaca gcgaggccag ctcactgagt gcggagccga
     2461 ccgatggatc atctgggccc gttgttttct cggcaatggc cagcacccgc tggaaaagcg
     2521 gctctgcttt gtcatattcg ccttgtgtct gatagagccc tgccaggttt tcgagccggg
     2581 taacaaccga tggatgatct ggccccagtg ctttttcttt gatggccaga gaccgcagat
     2641 acaacgcctc cgcccgtgta tagtcacctg tgtcctgata gagcctggcc aggttgttga
     2701 gcgtggtggc gactgatggg tggtccggac caagtgcttt ctcctgaatg gcaatcgccc
     2761 gcacaaaaaa cggctcggct ttggcgtact cgcctttttc ctgaacgagc agggccagat
     2821 cgtggagctt atcagccaga gccagatcct ctgggccaaa aatccgctca gcaatctcca
     2881 ctacccggag tgcgagcggc agagcttcat ccagtttttc tttattttgc aggtccgtaa
     2941 tctggatggt gagccggtct aagtcaattt ttgcccggtc ctggtcagtc gctggccgag
     3001 gcg
//
LOCUS       DAOQDO010000557         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26218_length_3001_cov_3.497624, whole genome shotgun sequence.
ACCESSION   DAOQDO010000557 DAOQDO010000000
VERSION     DAOQDO010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26218_length_3001_cov_3.497624"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..909
                     /gene="pheA"
                     /locus_tag="PKZ53_09960"
     CDS             55..909
                     /gene="pheA"
                     /locus_tag="PKZ53_09960"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNJ40802.1"
                     /translation="MIPLIAFQGEPGAYSEIAAGRLGRPEGRRRFVDVFEAVTAGQVA
                     LGAIPLENSLGGTIHENYDLLLKYPVRIVAETYVPINHCLMGLPQATLEQARQVLSHP
                     QALAQCDVFLASHPEWEIIPAYDTAGSAKMVREAGDPTRLAIASEQAAGHYQLQVLAR
                     NIASTTNNITRFGFIQAESNLSPLPVPQSSDLISTSRKTTLAFTLAHKTGSLFQALCV
                     FAFREINLTKIESRPFREQAFNYLFYADFIEPEDPKISARALDHLSEIAPFLKILGSY
                     PCVGTMEF"
     gene            complement(973..>3001)
                     /locus_tag="PKZ53_09965"
     CDS             complement(973..>3001)
                     /locus_tag="PKZ53_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ40803.1"
                     /translation="VTVEDTGSTNGVYVNGQRVSRCQVRPNDLVQIGPYTLQVDVSGI
                     VVFDSRSQSRIDVIDLTKEVKNRDGSGMIKLLDRIRLSIPANEFVGLIGPSGAGKSTL
                     MDALNGMRPADQGTVLVNNLNLYENINSFKQSIGYVPQDDIIHRELTVYRTLYYVAKM
                     RLSSDTSNAEIDNIINEVLDVTGLTSRRNVPISQLSGGQRKRVSIAVELITKPSIIFL
                     DEPTSGLDPGTEEKIMQLFRQISEAGHSVVLTTHAMENVKLFDKLVVMMRGRLVWYGP
                     PQECLEYFGINSIKHLFDTLGDPNSDQVAIGWQRKFEQSTAYQKYIIQPLSGLQRLPA
                     QPPRKATNDNTLWQSIRQMFVLARRYFEVMWADKFNLLILLGQAPVIALLMGLAVGND
                     WTRDFPYFILALSSLWFGCSNAAREIVKESNVYKRERMVNLGILPYIFSKLIVLVLIG
                     MMQCALLYGVGALVESVPGNPVLIYLCMLFSTMTGIGIGLLISAAVRTSEVATSLVPL
                     ILIPQILFGGLVLPNKGPSEAIGFAMPAMWSYDLLKHISDLDVMRGISDEYDEDGKIG
                     RVKAANDRAIKKFEDDLENYREEEQEKLTDYKRRADNAMYGGGGNPGPMPSLGDPPKA
                     PKIEYPPDDKSEFVGFRTTYGSIPLNFAILGIFFVVLLILTGLVLKSKDVL"
BASE COUNT          767 a          781 c          691 g          762 t
ORIGIN      
        1 attcttcatt ctcaattctt cattctcaat tcttcattct caattcttca ttccatgatt
       61 cctctcattg cctttcaagg tgaacccggt gcctatagcg aaattgcggc aggacgactt
      121 ggccgtcctg aaggccgccg ccggtttgtt gatgtctttg aagccgttac cgccggacag
      181 gtcgcgctag gtgctattcc gcttgaaaac agtctcggcg gaacaatcca cgaaaattat
      241 gatttgctgt tgaagtatcc ggtgcgaatt gtggcggaaa cttacgttcc aattaaccac
      301 tgtttgatgg gactgcctca ggccacttta gagcaagccc ggcaggttct ttcgcatccg
      361 caggcgcttg cccagtgtga tgtctttctg gcatcacacc cggaatggga aattattcca
      421 gcctatgaca cggctggaag tgccaagatg gtccgtgagg cgggtgatcc gactcgattg
      481 gctattgcca gcgagcaggc ggcggggcat taccagctcc aggtactggc tcgcaatatt
      541 gccagcacca ccaataacat cacccggttt ggctttattc aagctgaatc aaatctgtct
      601 ccgctccctg taccgcaatc atcagatttg atttcgacca gccgaaaaac gacgcttgcc
      661 tttaccctgg ctcataaaac cgggagctta tttcaagcgc tctgtgtgtt tgcctttcgt
      721 gaaatcaatc ttaccaagat cgaatccaga ccttttcgtg aacaggcgtt taactacttg
      781 ttctatgcag attttatcga acctgaggac cccaaaatca gtgcccgtgc ccttgatcac
      841 ttgagtgaaa ttgcaccgtt tctcaaaatt ctaggcagct atccctgtgt gggcacgatg
      901 gagttttgac aaggtgacaa ggtggctttt attcgaaacc ctgaaccctg aaccctgaac
      961 cctaaaccct gactaaagca catccttact ctttaaaacc agaccggtga gaatgagcaa
     1021 gacgacaaaa aagataccca ggatagcaaa attaagcgga atactgccgt aagtggttcg
     1081 aaatccaaca aattcacttt tgtcatcagg tgggtactca attttaggag ctttgggagg
     1141 atcgccaagc gagggcattg gacctggatt cccgcctcca ccatacatgg cattgtcggc
     1201 acggcgttta tagtcagtga gtttttcctg ttcttcctct cgatagtttt ccaaatcgtc
     1261 ttcaaatttc ttgatcgccc gatcattggc cgcttttaca cgaccaattt taccatcttc
     1321 gtcatattca tcactgattc cacgcatgac atccagatca ctaatgtgtt taagcaggtc
     1381 ataagaccac atcgccggca tggcaaaacc aattgcttcc gatgggcctt tatttggcag
     1441 gacgagtccc ccgaagagga tttgtggaat cagaatcaag ggcaccaggc tggtggcaac
     1501 ttctgacgtt ctgacagccg ccgaaatcag caagccaatc ccaatgcccg tcatcgttga
     1561 aaacagcatg cagaggtaaa tcagcaccgg gtttcctgga accgattcaa ccagagcgcc
     1621 aacaccatac aacaaagcgc actgcatcat accaatcaac accagcacga tgagcttgga
     1681 aaagatatag ggtaaaatcc ctaaattgac catgcgctca cgtttataga cattactttc
     1741 tttgacgatt tcgcgggcgg catttgagca tccaaaccag agcgacgaca aggccagaat
     1801 aaaatagggg aaatcgcggg tccagtcatt tccaaccgcc agacccatca acagggcaat
     1861 taccggagct tgtcccaaca agatcaacaa gttgaattta tctgcccaca tgacttcaaa
     1921 atagcgccgg gccagtacaa acatctggcg gatgctttgc cataacgtgt tgtcattggt
     1981 ggctttccgt ggtggctggg caggcaaacg ctgtaaccca ctcaatggct ggatgatgta
     2041 tttttgatag gcggtggatt gctcaaattt tcgctgccag ccaatcgcaa cctggtcgct
     2101 gtttggatca ccgagggtgt caaagaggtg tttaatgctg ttgataccaa aatattcgag
     2161 gcattcctgg ggtgggccat accataccaa ccggccacgc atcatcacca cgagtttgtc
     2221 aaacagcttg acgttttcca tggcatgggt ggtcaacaca accgaatgtc cggcctcgga
     2281 aatctgccgg aagagctgca taattttttc ttctgttcca ggatccagcc cggatgttgg
     2341 ctcatcgaga aagataatcg agggcttggt aatcaattcg actgcgattg aaacccgctt
     2401 gcgctgaccg ccggaaagct gggaaatcgg gacgttccgc cgtgacgtca aacctgtcac
     2461 atcaagcact tcgttgatga tgttgtcaat ttcagcattg gacgtgtcag acgacaaacg
     2521 cattttggcc acgtagtaca gcgtccggta gacagtcagt tcacgatgaa taatgtcatc
     2581 ctgggggaca tacccaatcg actgcttaaa gctgttgatg ttttcataca ggttgaggtt
     2641 attgaccagc accgtcccct gatcagccgg acgcatgccg ttgagggcat ccatcagggt
     2701 tgatttcccc gcgccagagg gaccaatcaa accgacaaat tcattggcag gaattgataa
     2761 gcgtattcga tctaataact tgatcatccc cgagccatcg cggtttttga cttctttagt
     2821 cagatcaatc acatcaattc gcgattggct tcgggagtca aaaaccacaa tccccgaaac
     2881 atcaacttgc aatgtatagg gaccaatttg gaccagatca tttggacgca cctgacatcg
     2941 gctcacccgt tgcccgttga catagactcc attggtgctg ccggtatctt ccaccgtcac
     3001 g
//
LOCUS       DAOQDO010000558         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26278_length_2998_cov_2.647978, whole genome shotgun sequence.
ACCESSION   DAOQDO010000558 DAOQDO010000000
VERSION     DAOQDO010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26278_length_2998_cov_2.647978"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..>2998)
                     /locus_tag="PKZ53_09970"
     CDS             complement(230..>2998)
                     /locus_tag="PKZ53_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ40804.1"
                     /translation="ATTLNNLAILHNQLGEFQAALTAYEEALKISIEFDDKESQGIIL
                     SNIGRVYDNQGNKQKALDYFRQAIPVKQVYGDKLSLAETVNNMGTVYIDLGETRLALE
                     HYTQALALCRESGQPFSEGILFNNLALAYNRLGEPQKALESYTQALAYNRKIGNRNAE
                     ALVLLNTGTVYLDLGESRKAIELFQQAQQIWHETENRQSEAMAFNNLGLGYSRISETS
                     LGIQYYLQSLELRKSLGDRFGQAVTLSNLGTVMVDAREYTKALEYLQQSLTIAQENDD
                     KESIALARQNIGAVLLRSGKPHEALASLTESVPLWQTTGNRSGTARALSLLAETEQIL
                     GRLLDAKAHFEQALEQVEFIRARAGSQDLRTSFFARIQNYYRLYINLLLQLHRENPLD
                     GYDRQAFQISEQARARSLLDLLAEAKLNIRQGADPQLLEHEQTLKKRITDKLERLSRL
                     LAGKHTETQKNEAEKEIGTLTDEYRQVQAEIRQKSPQYASLTQARIVTVEELQTQVLD
                     PDTIVLEYVLGAEQSHLFAVSQGNFAVYPLPGRDELTKAARQVYELLTKRSTALKELA
                     QERNRTDISPEDYQARVAAADRAYPQAAAELSRMLLGPVQKDIRGQRLVIVADGALQI
                     VPFAALPVPNPKPESKSTPYLIERHEIVVVPSASTLVALRRNRRSLGATINSVAVLAD
                     PVFTARDERAKGQNQSVAAQLPDTITRHLDQFSSPPATDESGNKVLQLRIPRLPGTRV
                     EAEAIRKITGDTSLIRLDFQANREAALNPELSKYRYIHLATHGYLNVSRPELSAVLLS
                     LINEQGETVDGFLRLEDLYNLNWPAEVVTLSACQTGLGTDIKGEGIVGLTRGLMYAGA
                     RRVVVSSWNVSDQATAQLMTRFYQGMLRDGNRPAAALRAAQIQMLGDKRWRAPYFWAA
                     FQLQGEWR"
BASE COUNT          595 a          799 c          824 g          780 t
ORIGIN      
        1 aatttatgag actttcctgt atacgattta atgatactca acagggttca gggttcaggg
       61 ttcagggaaa tacaattttt tcaatagttt cgcgttccca taatgcgaaa atttcatttg
      121 aaaatggtat gagaaccagg ttcttcagcc ccaagctctc agccccaagc ccttagccca
      181 atttcttcag cccccagccc ttagcccgat gttcttcagc cctgatcttt caccgccatt
      241 caccttgcag ttgaaacgcg gcccaaaagt acggcgcccg ccagcgtttg tcgccgagca
      301 tctggatttg ggcggcacgt aaggctgccg ccggacgatt cccgtcgcgg agcattccct
      361 ggtaaaaccg ggtcatcaac tgtgcggtcg cctggtcgct tacgttccac gaactcacca
      421 cgacgcggcg cgctccggca tacatcaggc cacgggtcag gcccacgatg ccttcacctt
      481 tgatgtcggt gccaagtccc gtctggcacg cgcttaacgt taccacttcg gctggccagt
      541 tgaggttata gaggtcttcc aggcggagaa atccatccac tgtctcaccc tgttcattga
      601 tcagcgacaa caacacggct gacaattccg ggcggctgac attcaaatag ccgtgtgtgg
      661 ccagatggat atagcggtat ttgcttagtt cagggttcag cgctgcttcg cgattggcct
      721 ggaaatccag ccggatcaaa ctggtgtcac ctgtgatttt tcgaatcgct tcggcttcga
      781 cgcgggtgcc gggcagacgg ggaatccgca gttgcagcac tttattaccg gattcatcag
      841 tcgctggcgg cgaactaaac tgatccagat gccgggtgat ggtgtctgga agctgggccg
      901 ccaccgactg gttctgccct ttggcccgtt cgtcccgagc ggtaaacacc gggtcggcca
      961 gcacggctac gctattgatg gtggctccca ggctgcgtcg gttccggcgt aacgccacca
     1021 gcgtcgaagc cgatggcacg accacgattt catgacgttc gatcaggtac ggcgttgatt
     1081 ttgattcagg tttgggattg gggacgggaa gggccgcaaa gggaacaatt tgcagcgccc
     1141 catctgccac aatcaccagt cgctggccgc gaatgtcctt ttgaaccggc cccagcagca
     1201 ttcgactcag ttctgccgca gcttgtggat aggcccggtc agcggcagcc acccgcgcct
     1261 gatagtcctc gggtgaaata tcggtgcgat tgcgttcctg ggccagttct ttgagcgccg
     1321 tgctgcgttt ggtcaagagt tcgtacacct gccgggccgc tttggtgagt tcgtcccgtc
     1381 ccggcagtgg atacacagca aaattgccct gggaaaccgc aaacagatgg ctttgttcgg
     1441 cgcctaaaac atattcgagc acgatggtgt cgggatccag cacctgggtt tgaagttctt
     1501 cgaccgtgac gattctggcc tgggtcagcg aggcatactg cgggcttttc tgacgaattt
     1561 cagcctggac ctgccggtat tcatcggtca gtgttccgat ttctttttcc gcttcgttct
     1621 tttgcgtttc ggtatgtttc ccagcgagca ggcgcgagag tcgttcaagt ttatctgtga
     1681 tccgtttttt gagggtttgt tcgtgttcga ggagttgcgg atcggccccc tgtcgaatgt
     1741 tcaactttgc ttcggccagc aaatccagga gcgaccgggc gcgggcctgt tcgctgattt
     1801 gaaacgcctg acggtcatag ccatccagcg ggttttcgcg gtgcagttgc agcaacaggt
     1861 tgatatagag ccggtaatag ttctgaatcc gggcaaaaaa cgaagttcgc aaatcctggc
     1921 tgccggcacg ggcacgaata aattcaacct gttcgagcgc ctgttcaaag tgagctttgg
     1981 cgtcaagcaa ccggcccagt atctgctcgg tttccgccag caaactgagc gcccgggcgg
     2041 tgcctgaccg gttgccggtc gtttgccaga gcggaactga ctcggtgagt gatgccaggg
     2101 cctcgtgtgg tttccctgac cggagcaaca ccgctccgat gttttgccga gcaagggcaa
     2161 tgctttcctt atcgtcgttt tcctgagcaa tggtcagact ttgctgaaga tactcaagtg
     2221 ctttggtgta ttctctggca tcaaccataa ccgtacccag gttactaagg gtgactgcct
     2281 gtccaaaccg gtctccaagg ctttttctga gttccagtga ttgcaggtaa tattgtatcc
     2341 cgagcgatgt ttccgaaatt cgggagtagc ctaagcccag attattgaag gccatcgctt
     2401 ctgactgccg gttttcggtt tcgtgccaga tttgctgggc ttgttgaaac aattcaattg
     2461 ctttgcgtga ttctcccaga tccaggtaga ccgtgccggt gtttaagagc accaatgctt
     2521 cggcattgcg atttccaatt ttgcgattat aggccagggc ctgagtgtaa gattccaggg
     2581 ctttttgtgg ctcacccagt cggttgtacg ccagggcaag attgttgaac aggattccct
     2641 ccgaaaaagg ctggccgctt tcccgacaca gggccagtgc ctgggtgtag tgttcgagtg
     2701 ccagccttgt ctcacccaga tcaatataca ccgtccccat attattgacg gtttcggcca
     2761 gactcagttt atcgccatag acttgtttga ctggaatggc ttgccggaaa tagtccaggg
     2821 ctttttgttt gttcccctga ttgtcataaa cccggcctat gtttgagaga atgatgccct
     2881 gggattcttt atcatcaaat tcaatcgaga ttttcagggc ttcttcatag gctgtgagtg
     2941 ccgcctgaaa ttcgccaagc tggttgtgta gaattgccag attgttgagt gtggtggc
//
LOCUS       DAOQDO010000559         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26296_length_2997_cov_2.852481, whole genome shotgun sequence.
ACCESSION   DAOQDO010000559 DAOQDO010000000
VERSION     DAOQDO010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26296_length_2997_cov_2.852481"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..317
                     /locus_tag="PKZ53_09975"
     CDS             57..317
                     /locus_tag="PKZ53_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNJ40805.1"
                     /translation="MRLGIVSALAVNESLTFNDLKKLMNTTDGNLSVHARKLEEANYI
                     TCTKFFDGRLPKTEYRLTQSGRDALERYLNHMETLIQAMRPR"
     gene            350..1084
                     /locus_tag="PKZ53_09980"
     CDS             350..1084
                     /locus_tag="PKZ53_09980"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HNJ40806.1"
                     /translation="MKQSTLIKTELHGIHVAIIMDGNGRWATQRGLPRVAGHQAGVET
                     VRRIVEAAPSLGINILTLYAFSSDNWKRPAPEVTALMQLLRIFLKREAMRCARQGVRL
                     EVLGRRDRLPAAVVEEITRAETLTRQGTRLHLRIAVDYSSRDAILQAVQQVATLPELT
                     RDTFATLLGQVHNSQSPAPDVDLLIRTSGEQRLSDFLLWECAYAEFFFTKSHWPDFQP
                     AELEAAVSDFRRRDRRFGALPQAVAS"
     gene            1201..2697
                     /locus_tag="PKZ53_09985"
     CDS             1201..2697
                     /locus_tag="PKZ53_09985"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNJ40807.1"
                     /translation="MDSKMRLGLEVLVISLLNGLTADLLLRELPWGANIFLWVAGFGG
                     SLLFLSYRWRQEKIPFDHGLVLMAVVFSAAGAWRDSPTLRGLNLMVTAIAVGLLARPQ
                     LLLHLRRAGVIEYFLNLILAWLSPMIGPTFVFSSEMEWKQIPRSKWTSQASAIVRGAI
                     IAFPLLLVFGGLLVSADATFERIVNHFFNFDPAELIVHAGLTLVVAWLTGGLIQRMFL
                     DNGPGLAPSTLLPLGLRNLGITEVATVLSLLNLLFLSFVLIQIRYFFGGAARVFTTSG
                     LTFAEYARRGFFELVTVAALVLPVLLALHWMLNQENPAHERLFRWLAGFQIVMLLVMM
                     TSALQRMMMYQKMYGLTELRVYTTAFMLWLAVVFVWFGMTVLRGQRQAFAFGALMMAI
                     IAIGTLNVINPDALIAGTNLARVSEGRTVDAVYLTRLSADAVPVVVNHLETIHLSQRV
                     RVADWLITRWTPDEKITWRSWNLSRSKAWQKVYDRQTELCALACQPVE"
BASE COUNT          630 a          783 c          791 g          793 t
ORIGIN      
        1 ggaaagcaac cactgaacca agcgcgcttg aactcgaccg attgattcac gaacgcatgc
       61 gcctggggat cgtgagtgca ctggcggtca atgaatcatt aacttttaac gacctcaaaa
      121 aattgatgaa caccacagac ggaaacctga gtgtccatgc tcgcaagctt gaagaagcca
      181 attacattac ctgcaccaag ttttttgatg gacgactgcc caaaactgaa tatcggctta
      241 cccagtctgg ccgcgatgcc ctggagcggt atctcaacca tatggaaaca ttaattcaag
      301 ccatgcgccc ccgttagggc gctggcaatt ttttttggag gtttacttta tgaaacaaag
      361 cactttgatc aaaacagaac ttcatggaat tcatgttgcc attattatgg acggcaacgg
      421 gcgctgggcc acccaacgcg ggctaccacg cgttgccggt catcaggctg gggttgaaac
      481 cgtccgccgg attgttgaag cagcgccatc gctcggcatc aacatcctga ccctctatgc
      541 cttctcgtca gacaactgga aacgcccggc gcccgaagtc acggctctga tgcagttgct
      601 gcggatcttt ttgaagcgtg aagccatgcg gtgcgcccgc cagggagtcc gccttgaagt
      661 tcttggccgc cgggatcggc tccctgccgc cgtggttgaa gaaatcaccc gggctgagac
      721 cctcacccgc cagggcaccc ggttgcacct gcggattgcg gttgattact cctcccgtga
      781 cgccattttg caggcggttc agcaggtagc cacccttcct gagttgaccc gcgacacctt
      841 tgccaccctc cttggccagg tccataacag ccagtcacca gcacctgacg ttgatttgtt
      901 aattcgcacc agcggcgaac aacggttgag cgattttctg ctttgggaat gcgcctatgc
      961 cgaatttttc tttaccaaaa gccactggcc tgatttccaa ccggctgaac ttgaagcagc
     1021 cgtgagtgat tttcggcggc gtgaccgccg attcggagcg cttccacagg cggtggccag
     1081 ctaattggcc ctgtaaatca ggcacctgca attgatttca aaagacgata gaactcattt
     1141 gaaatcaaca atttcagaat tcattgaatt gaactttggt tttgttagga gtaaccggtt
     1201 atggattcaa aaatgcgact tggattagaa gtactggtca tttccctgct caatggtctc
     1261 accgcggatt tgctgctccg ggaactcccc tggggcgcca atattttttt atgggtggcc
     1321 gggtttggag gatcgttgct ctttctgagc taccgctggc gacaggaaaa gatcccattt
     1381 gaccacgggc tggtgctgat ggcggtggtg ttttcagccg ccggggcctg gcgtgattcc
     1441 ccgaccctcc gaggactcaa tttgatggtt acggccattg cggtgggctt gctcgcccgg
     1501 ccacaattgc tgcttcacct gagacgggct ggtgtgattg agtattttct caatcttata
     1561 ctggcgtggc tctcgccaat gattggtccc acttttgttt tttcttctga aatggaatgg
     1621 aaacaaatcc cacgctcaaa atggaccagt caggcatcag ccatcgtgcg tggggccatc
     1681 atcgcgttcc ctttattgct ggtgtttgga gggttgctgg tctcagccga cgccacgttt
     1741 gaacgaattg tcaatcactt tttcaatttt gacccagccg agttgattgt tcacgccggg
     1801 ttgacgctcg ttgtggcctg gctgacaggt ggactgattc aacgaatgtt tcttgataac
     1861 ggtcctggac tcgcaccgtc aacgctgcta ccgcttgggt tgcgcaacct cggcattacc
     1921 gaagttgcca cggttctctc cttattaaac ctcttatttc tcagctttgt attgattcag
     1981 attcgctatt tctttggagg cgcggcacga gttttcacaa cctctggatt gacctttgcc
     2041 gaatatgccc ggcgtggttt ttttgaactg gtgacggtcg cggcactggt tctgccagtt
     2101 ttgctggccc tgcactggat gctcaatcag gaaaatccgg ctcacgagcg attgtttcgc
     2161 tggctggctg ggtttcaaat cgtgatgctg ctggtgatga tgacatccgc gctccaacga
     2221 atgatgatgt atcaaaaaat gtacgggtta accgaactgc gcgtctacac aacggcattt
     2281 atgctctggc tggccgtggt ttttgtgtgg tttggaatga cggtcttgcg tgggcaacgc
     2341 caggcttttg catttggggc actgatgatg gcaataattg ccataggaac gctcaatgtc
     2401 atcaacccgg atgccctgat tgccggcacc aaccttgctc gggtttctga aggccgtacg
     2461 gttgacgccg tttatctaac ccgactgagt gcagatgcgg ttccagtcgt ggtcaatcac
     2521 ctcgaaacca tccatctttc acagcgtgtc agggtggcgg actggctgat cacccgctgg
     2581 acaccagatg aaaaaataac ctggcgatcc tggaatctct cccgctcaaa agcctggcaa
     2641 aaggtatatg accgccaaac cgaactgtgt gctctggcct gccaaccggt ggagtgaaga
     2701 attgagaatg aagaatgaag aatgaagaat gaagaaaatt cttggttcct ggttcctggt
     2761 tcttggttct tggttcctgg ctcttggttc ttggctcttg gttcttggct cttggttctt
     2821 ggaaagcgtt ggttactagg ttctgttgac atttcttcgc cgcgtagcgg tggctgaatt
     2881 gagaccggtg cttcagcgcc gggaacgatc aaatttggga tccatcgccg ctccatcatg
     2941 gtcattttcg tttcccagcc ttgaaaggcc ggtctcaact cacggctccg cgacgcg
//
LOCUS       DAOQDO010000560         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26357_length_2993_cov_3.384275, whole genome shotgun sequence.
ACCESSION   DAOQDO010000560 DAOQDO010000000
VERSION     DAOQDO010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26357_length_2993_cov_3.384275"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKZ53_09990"
     CDS             complement(<1..611)
                     /locus_tag="PKZ53_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ40808.1"
                     /translation="MSHPQSRSQTPVQTETPPIIIQADGLKRVYATRPESLRIFEDLA
                     LNVVQGESLAITGPSGVGKSTLLHILGGLDRPTGGKVEIGGTSIWELSASALHRFRNQ
                     NIGFVFQFHHLLPEFTAQENIQMPMLIAGLSLKESARRATELLDRIGLKARAHHRPSE
                     LSGGESQRVALARALANNPKVLLADEPTGNLDERTGESIQELLR"
     gene            1008..>2993
                     /locus_tag="PKZ53_09995"
     CDS             1008..>2993
                     /locus_tag="PKZ53_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNJ40809.1"
                     /translation="MKWNQFQSFKRQILFIGCLLLAGILVFSHLTRQTDAAAYRRLSG
                     RVASGVQIPTIVPLVQEGAPAPCAQIQRFDTPTGVPLTIPDGGGGGCILQVVTSTITI
                     SGAGEYLQDLNLQTFIRHPFCSDLHITLTSPQGTTATVTSKNAGGGPRANVFNGTVWD
                     DQANPGGQVPYTNNDGLVTDRLYVTNVLASPLVPEEGFAVFRGENPNGVWTLTIGDDF
                     VGPTLDGLGTLDDWSIELTSLASAPMETTSGVFANATPMTIPVPPVTGPNVVTSTINV
                     ASVTGFLSDITLTTDISHTRCQDLDITLTSPGGTVVTITTDNAGSAQNVWGSIPLSGT
                     ARQTIWSSTADNRVANEPDGSSNCAGFQDGDFIECGLAGARCDELPFRSFRQGSGFFF
                     NVYYNNGTVADHRYVTNELASLLTPEESFGVFDGEDPNGTWTLTISDDTRTSGGTLNN
                     WSLQFITRDTADFNGCIELPVLYVADTVNHRIQTFDGAAWARIGQAGPGSLAGQFRSP
                     EAVTASVDGQTIYVADTGNNRIQVSTDAGATWAVIATLGSALNQVRSPSGIALDTQGN
                     LYIGDTGNNRVVRYTGGNFLQATILASLGSGPGQVRTPHGLVVDPNFRLFIADFGNKR
                     VLRLDGANLETLMNQAATVATSGAAPNQVNAPQGVGTD"
BASE COUNT          736 a          713 c          744 g          800 t
ORIGIN      
        1 cgcagcaact cttgaatact ttccccagtt cgttcatcca gatttccggt tggttcatcg
       61 gccagcagca cctttggatt attggccagt gcccgtgcca gtgccactcg ctggctttcg
      121 cctcctgaaa gttccgatgg gcgatgatgg gcacgcgcct tgagcccaat ccggtcaagt
      181 aattcagttg cccgacgggc gctttctttg agtgagagcc cggcaatcaa cattggcatt
      241 tgaatatttt cctgagccgt aaattccggt aataaatgat gaaactggaa gacaaaccca
      301 atgttttgat tgcgaaaccg gtggagcgcg ctggctgata attcccaaat cgaagttcca
      361 ccaatttcaa ctttgccgcc agttgggcgg tcaagtcccc ccaggatatg gagcaaggtt
      421 gattttccca cccctgacgg cccggtaatc gccagtgatt caccctggac aacattgagt
      481 gccagatcct caaaaattcg aagcgactca ggtcgggtgg catacacccg cttcaagccg
      541 tctgcctgga tgatgattgg gggagtctct gtctgaactg gtgtttgtga acgtgattga
      601 gggtgactca ttggataacc tcgctactat ttgggcaata agtaagatat taatttcgtg
      661 ataaatcgaa tgatttacta gctgatttct tctgaattat ggagatttga tttagtaaaa
      721 atacatcttg cctggagttt tttttaacca gcgatatgaa atttgcagtt taaaaaattg
      781 aaaataaatg tcttatttgt gaattcaaaa ttttagattt gaatagatta taagaatagt
      841 atgttttata tcaatccatt tttttgaatt tgcaccgata ttacctcttc gaattaccct
      901 attttatttt gagataattg tgatacatgt gaagtgccag ccgacaaatg aatcgaaaga
      961 tcaattcatt aaacttcata ccgaaactaa aaaggagtga tctgtgtatg aaatggaatc
     1021 aatttcaatc tttcaagcga cagattcttt ttattggttg cctgttgctg gctggcattt
     1081 tggtgttttc acaccttaca cgccaaaccg atgcggcagc ttaccggcga ctctctggcc
     1141 gcgtggcttc tggcgtacaa attccaacaa ttgttccatt ggttcaggaa ggagccccgg
     1201 ccccatgtgc acaaatacaa cgatttgaca ccccaacggg tgttccgttg accataccgg
     1261 atggaggcgg aggcggctgt attttacaag ttgtgacttc gaccataact atcagcggtg
     1321 ccggagaata cctgcaggat ctgaatttgc aaacctttat ccgtcaccct ttctgtagtg
     1381 atttgcatat tacgctcacc tcgccacaag gtacgacagc caccgtaacc agtaaaaacg
     1441 ctggtggagg acctcgtgca aatgttttta atggaacagt ttgggatgac caggcaaatc
     1501 caggtggtca ggtcccctat acgaataacg atgggcttgt aactgatcgt ctatatgtaa
     1561 ccaatgtcct tgcctcgcct ctggtaccag aagaggggtt cgccgttttt cgtggagaaa
     1621 acccaaatgg agtgtggacc ttaaccatcg gagatgattt tgttgggcct acgctggacg
     1681 gactcggcac tctagacgac tggtccatag aacttaccag tcttgcttcg gccccgatgg
     1741 aaaccaccag cggtgtgttt gccaatgcca caccaatgac aattccagtc cctcctgtaa
     1801 ctgggccaaa tgtggtgact tcgaccatca acgtcgcgag tgtcactgga ttcctcagtg
     1861 atataacctt gaccactgat atttcacaca cccggtgtca ggatttagat atcaccctga
     1921 catcaccagg tggcactgtg gttaccatca ccactgataa tgctggaagc gctcaaaacg
     1981 tgtggggaag cattcctctt tctgggacgg caaggcagac gatttggagt tcaaccgctg
     2041 ataatcgagt tgctaacgag cctgatggaa gtagtaactg tgccggattt caggacggtg
     2101 attttattga atgtggtctg gctggagctc ggtgtgatga acttccattt cggtctttcc
     2161 ggcaaggaag cggcttcttt ttcaatgtgt attacaacaa tggaaccgta gccgatcacc
     2221 ggtatgtgac caacgaactg gccagtctcc tgacgcccga agaatcattt ggggtgtttg
     2281 atggggagga tccaaatggc acctggacac ttaccatcag tgatgacacg cgcaccagtg
     2341 gtggaacact caacaactgg tcgctccaat ttatcacgcg tgacacggcg gatttcaatg
     2401 ggtgtattga gttacctgtt ttatatgtgg cagataccgt caatcatcga attcagacct
     2461 ttgacggagc cgcctgggcg cgtattgggc aagcgggtcc aggttcgctg gcgggtcagt
     2521 tccgtagccc cgaagcagtt actgccagtg tggatggcca aaccatttat gtggcagata
     2581 ccggaaacaa ccggattcag gtctcaaccg atgccggagc gacctgggcc gttattgcca
     2641 cgcttgggtc ggcccttaat caggtgcgat caccctctgg cattgcgctg gatacccaag
     2701 gcaatctcta tatcggagac actggaaaca accgggtggt gcggtataca ggtggcaatt
     2761 tcctgcaagc cacgattttg gcgtcacttg ggtctggtcc agggcaggtt cgaacaccgc
     2821 atggcctggt cgttgaccca aacttccggc tttttatcgc cgattttggt aataaacgag
     2881 ttctgcgact tgacggggcc aaccttgaaa ccttgatgaa tcaggcggca acagtggcca
     2941 cttcgggggc ggctccaaac caggtaaacg ctcctcaggg cgttggcact gat
//
LOCUS       DAOQDO010000561         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26494_length_2986_cov_2.664961, whole genome shotgun sequence.
ACCESSION   DAOQDO010000561 DAOQDO010000000
VERSION     DAOQDO010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26494_length_2986_cov_2.664961"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PKZ53_10000"
     CDS             <1..1190
                     /locus_tag="PKZ53_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNJ40810.1"
                     /translation="IGSLTQMASIRLGKRTEGRNPRIKDFVPLAELDDLVFGAWDIFE
                     DSAYEAAVHAGVLERTQLDQLKPALEAIKPMPAVFDQHYVKRLNGTNIKQGANKLELA
                     QQLIADIEDFRQKNDCSRLVLVWCASTEVFLQPDPVHDTIASFEQAMKDNHPAIAPSM
                     IYAYAALKCKVPFANGAPNLTVDLPAIQQLAAENLVPICGKDFKTGQTFMKTLLAPGL
                     KSRMLGLHGWYSTNILGNRDGEVLDDPESFKTKEESKLSVLEYILQPELYPELYKDFS
                     HVVRINYYPPRGDNKEGWDNIDIFGWLGYPMQIKINFLCRDSILAAPIVLDLALFLDL
                     AQRVGMKGIQEWLSFYFKSPMTAPGLYPEHDIFIQLMKLKNTLRDLRGEELITHLGKE
                     YYD"
     gene            1397..2716
                     /locus_tag="PKZ53_10005"
     CDS             1397..2716
                     /locus_tag="PKZ53_10005"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNJ40811.1"
                     /translation="MRTPVEINQLKRDGHTLSSEDITNLVNAYTRDEVPDYQIAAFLM
                     AVCCRGMVTEEIQALTNAMLHSGMVLDFSDIPGIKVDKHSTGGVGDKTSLVLAPLVAA
                     AGIPVPMISGRGLGHSGGTLDKLESIPGFQTGLPLTEFRRLLSTIGVGLIGQTLEIAP
                     ADRKLYALRGATGTVESVPLITGSIMSKKLAEGIDALVLDVKCGNGAFMKTTDRALEL
                     ARSLIATGEGMGKQVTALVTDMNQPLGWSVGNALEVIEAQEMLRGNLVGRFAQLTLEL
                     AAHMIQLGSKVPYLKQARDLAVDLITSGKAMEKWQQIIEAQGGDPRVTEDETRLPQAA
                     HQKIMRAEQSGILTAIETEAVGWASLLLGAGRLQLSSPIDPAVGIRIHVERGAEMKAG
                     DPLCTLYYNDSAKLALAEERLKTVFTIVPGADEFHRTTLAPEWFVVQ"
     gene            2761..>2986
                     /locus_tag="PKZ53_10010"
     CDS             2761..>2986
                     /locus_tag="PKZ53_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40812.1"
                     /translation="MTNDKVTNWPKNYFIRKFSCCVGWRWLVTLLLTVTLAGLGLTGC
                     GIRERINKDRAETILKKGKVYQVLAQQEPDT"
BASE COUNT          752 a          774 c          781 g          679 t
ORIGIN      
        1 caattggatc actcacccaa atggcgtcta tccgccttgg gaaacgcacc gaaggacgca
       61 accctcgcat taaagacttt gtgccgctgg ccgaacttga tgatctggtg tttggcgcct
      121 gggatatttt tgaagactct gcctatgagg cggcggtcca tgccggcgtt ttggaacgaa
      181 cccagcttga tcaattaaaa ccggcgttgg aagccatcaa acctatgcca gccgtgtttg
      241 accagcatta tgtgaaacgg ctgaacggaa ccaacatcaa acagggcgcc aacaagcttg
      301 aactggccca acagttgatc gccgatatcg aagattttcg ccagaaaaac gattgttccc
      361 gcctggttct ggtctggtgt gcttcaaccg aagtgtttct ccaaccggac ccggtccacg
      421 acacaattgc cagttttgaa caggcaatga aagacaatca cccggcgatt gcaccgtcaa
      481 tgatctatgc ctatgccgcg ctcaagtgca aagtgccatt tgccaacggg gcgccaaacc
      541 tgacggttga ccttccagca attcagcaac ttgcggctga aaatctggtg ccaatttgcg
      601 gcaaagactt taaaacaggc cagaccttta tgaagaccct gctggcaccg ggcctgaaat
      661 cacggatgtt gggtctgcac ggctggtatt cgaccaacat cctgggcaac cgcgatggtg
      721 aagttctgga tgacccggaa tcattcaaaa ccaaagaaga atccaaactg tcggtgctgg
      781 aatatattct gcaaccggaa ctctatccgg aactgtacaa ggatttttca cacgtcgtgc
      841 gcattaacta ttacccacct cggggtgata acaaagaagg ctgggataac attgatatct
      901 ttggctggct cggctatccg atgcagatca agatcaattt cctgtgtcga gattcgattc
      961 tggcggcgcc gattgtcctg gatctggcgt tgttccttga tctggcacaa cgtgtaggga
     1021 tgaaaggaat tcaggaatgg ctgagtttct acttcaagag cccaatgaca gcaccgggtc
     1081 tctatcctga gcacgatatt ttcattcaat tgatgaaact caaaaacacc ttgcgtgatc
     1141 tgcgcggtga agaactcatc acccacttag gcaaagaata ctacgattag ggcttagggc
     1201 ctgggggttt caacagcaga tttgatcggt tgttggtccc ccttgcgcct gcctgattct
     1261 tttccttctc cctcactcca ctctgaaatc atccttttgg atttagaaaa gacgaagcga
     1321 ttgataggat tgtctttctc tgaaccctga accctgaacc ctgaaccctc aagggtaagc
     1381 gtctagtccc cctaaaatga gaactccagt cgaaatcaac cagctcaaac gcgacggtca
     1441 caccctgtct tctgaagaca tcaccaatct tgtcaatgcc tacacccgcg atgaggtgcc
     1501 cgattatcaa attgcggcgt ttctgatggc ggtgtgttgc cgagggatgg tcaccgaaga
     1561 aatccaggcg ctgaccaacg ccatgcttca ttcaggcatg gtgctcgatt tttcggacat
     1621 tccgggaatc aaagttgata aacacagcac tggcggggtg ggtgataaaa cgtcactggt
     1681 gctggcaccg ctggtggcgg cggctggcat tccagtgccg atgatcagtg gtcgtggtct
     1741 tggccatagc ggtggaacgc tggacaaact ggaatctatt cctgggttcc aaacaggact
     1801 gccgttaacc gaatttcgcc gcctgctctc aaccatcggg gttggattga ttggacaaac
     1861 ccttgaaatt gccccggctg accgcaagct gtatgccctg cgtggcgcca ccggaacggt
     1921 tgaatcagtc ccgctcatta ccggcagcat tatgagcaag aagctggccg aaggaattga
     1981 tgccctggtg ctggatgtca aatgtggcaa tggtgccttt atgaaaacaa ccgaccgggc
     2041 gctggaactg gcccgctcgc tgattgccac cggcgaagga atggggaaac aggtaacagc
     2101 actcgtcacg gatatgaatc aaccgctggg ctggtcggtt ggcaatgccc tcgaagtcat
     2161 cgaagcccag gaaatgctgc gtggtaacct cgttggacgg tttgcccaac tgaccctgga
     2221 actcgctgcg cacatgattc aacttggcag taaggttccg tatctgaaac aggcccgtga
     2281 tctggcagtg gacctcatca cctctggtaa agccatggaa aaatggcagc aaattatcga
     2341 agcccagggc ggtgacccgc gcgtgaccga agacgaaaca cgattgcccc aggcagccca
     2401 tcaaaaaatt atgcgcgctg agcaaagcgg aatcctcacg gccattgaaa ccgaagcggt
     2461 tgggtgggcc tcactgctgc tcggcgctgg ccggttacaa ctcagttcac cgattgaccc
     2521 ggcagtcggt attcgcattc acgtcgaacg cggtgctgag atgaaagccg gtgatccact
     2581 ctgcacgctg tattacaatg acagcgctaa actggcactg gccgaagaac gcctcaaaac
     2641 cgtctttacg attgtccctg gcgcggatga atttcaccgc acaaccctgg cacctgaatg
     2701 gtttgtcgtc caatagagaa gttttgtatc gggggtggat gccgcgccca aagtgacaaa
     2761 atgacaaatg acaaggtaac aaactggcca aaaaactatt ttatccggaa attttcgtgc
     2821 tgtgtgggtt ggcgatggct ggtaactcta ttgctaacag ttactctggc agggttgggt
     2881 ttaacgggat gcgggatccg ggaacgaata aataaagatc gggctgaaac catcctcaaa
     2941 aagggaaaag tgtatcaggt gcttgcccag caggagcccg acacct
//
LOCUS       DAOQDO010000562         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26570_length_2980_cov_3.105299, whole genome shotgun sequence.
ACCESSION   DAOQDO010000562 DAOQDO010000000
VERSION     DAOQDO010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26570_length_2980_cov_3.105299"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..>2980)
                     /locus_tag="PKZ53_10015"
     CDS             complement(53..>2980)
                     /locus_tag="PKZ53_10015"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40813.1"
                     /translation="DRMLRKVADWMRVLLRPATVIGRIGGSEFGVLFLASGTKSAAES
                     ATQFTEACAAQVFAVEESDTEISLKTSVTLFPTESNHAFGLLAKGRKAVQVASHRPED
                     PASRHLRFEQFVGRALESEQLLHEFNLVKVGRGHPVFVSGAAGIGKTRLIQEFEWSLA
                     GQDVLFLTTRFYEAGGSLPYRAFYESLRGVIGYFLEVAENRLPALFGPMTPQVIQDFS
                     EGEWLTFASPSVQLSADQEKYRIFDYLTRLYLGVTRLRPVVLVIDDMHWADGLSLEFL
                     AYLMRNSIGHRLFLIATMREREATSLDFPITGWLRQMSRSGNYVLLTLRPLDDASMQF
                     VIEQTFGKEGIPPKVVATLLHEARGNPFYLREMLRLLVSDGAIKLERDHWVCDNIPEI
                     RLPETIVDLVDAYLSGLDGQTLEVLTQAAAIGGEFTYEVLEAVTEMKESALLPILEKA
                     LKNAILQEHPSRREDRYVFAHATFRKVLYERLSKRRRKRLHLHIGNVMAQVYAERSSF
                     IAAELARHYLAADDLEHALWYTIEAGNQSWSSLAISDAGRYYQQAEEIIERLGSAPLE
                     GDTPVLQMAALGLQVEGMKLAEYHLNFGCMCIQQGKVEKARLELDQAFGFGKQLDSNS
                     LMGRALTAMAELCHVLGDYATGMEHAADALDLLRQAGDNAGEIKALIAIAASHNFQGQ
                     YAQSRNVYEMVLDLAKAAGDRLSEAGALWGVGQSLYQQGHYESALKYGEQSLVITREI
                     GDRLGERRSLSLIGGVHLDLGNYDEALKYFESALRVVRGVGYRVSEGAMLNNIGEAMR
                     RQRRFTEALVYYQQSLELARETNDRGLQALVTLNIGLVQQGRGQHRQALEMFDQSLQE
                     ITYVGLWSLEYEAYICIGDSHLAIRNYAQARQAYETALQNCEVTGAKRHQWKALYGLA
                     HCERNLGNAPTALRLLRETVASIEALAAGLTSYTDRNRFLKDLHRVYNDLAELAVELG
                     EDI"
BASE COUNT          719 a          865 c          711 g          685 t
ORIGIN      
        1 cattctcaat tcttcattct caattcttca ttctcaattc ttcattctca attcaaatat
       61 cttcaccaag ttcaaccgcc agttctgcca aatcattgta cacacggtgc aaatccttca
      121 gaaaccgatt gcggtcggta taggaggtca aaccggcggc cagtgcttca attgaggcaa
      181 cggtttcacg cagtaagcgc aaggcggtgg gggcattgcc caaattccgc tcacagtgag
      241 ccagtccata cagggccttc cactgatgcc gtttggcccc ggtgacttca cagttttgaa
      301 gggcagtttc ataggcttgc cgggcctggg catagtttcg aatggcaaga tggctgtcac
      361 caatacaaat ataggcttcg tattcgagcg accagagccc gacgtaggta atttcctgca
      421 ggctctggtc aaacatttcc aatgcctgtc ggtgctggcc acgcccctgt tgaaccagtc
      481 caatattgag cgtcaccaga gcctgcaacc ctcgatcatt ggtttcgcgc gccagttcca
      541 gactttgctg ataatagacc agggcttcgg taaaccgacg ctggcggcgc atggcttcgc
      601 caatgttatt gagcatagcc ccttcgctga cacgataccc aactccacgc acaactcgca
      661 gggcagattc aaaatatttg agcgcttcat cataattgcc cagatccaga tgtactccgc
      721 caatcaggct caggctgcgt cgttccccaa gccgatctcc gatttcacga gtaatgacca
      781 gtgattgctc accatatttc aaggcggatt cataatgtcc ctgttgatag aggctttgtc
      841 caactcccca cagagccccg gcttcactca ggcgatctcc ggcggctttg gccagatcaa
      901 gcaccatttc gtagacattg cgcgactgag catactgccc ctgaaaattg tgcgaagcgg
      961 caatagcaat cagtgccttg atttcgccgg cattgtcccc agcctgacgc agcaaatcca
     1021 gggcatcggc ggcgtgctcc atcccggtgg cataatcacc aagcacgtgg cacagttcag
     1081 ccattgccgt cagcgcccgt cccatcagcg aatttgaatc gagttgtttt ccaaacccaa
     1141 atgcctggtc cagttccaga cgagcctttt caacttttcc ctgctgaatg cacatacaac
     1201 cgaagttgag gtgatattca gccagtttca tgccttcgac ttgtaacccg agtgccgcca
     1261 tttgcaacac cggggtatca ccttcaagcg gagcactccc cagacgctca atgatttctt
     1321 cggcttgctg gtaatatcgc ccggcgtcgc tgattgccag gctggaccag gactggttgc
     1381 cggcttcgat ggtgtaccag agggcatgtt ccagatcatc cgctgccaga tagtgacgtg
     1441 ccagttcagc agcaataaaa gaagatcgtt cggcgtaaac ctgggccatg acgttgccga
     1501 tatgcaggtg cagccgtttt cggcgacgct tgctcagacg ttcatagagc acttttcgaa
     1561 acgttgcatg agcaaacaca tagcggtctt cgcggcggct ggggtgttcc tgcaaaatgg
     1621 cattttttaa ggctttttcc agaatcggca acaaggctga ttccttcatt tcggtgacgg
     1681 cttcgaggac ttcataggta aattcacccc caatcgccgc cgcctgggtc agcacttcaa
     1741 gggtttgccc gtccagacca cttaaatagg catcaaccag atccacaatg gtttccggca
     1801 gcctgatttc cggaatgttg tcacaaaccc agtgatcgcg ctcgagcttg atcgccccat
     1861 cactgaccag caaccgcagc atctcgcgca gataaaatgg atttcctcgg gcttcgtgca
     1921 ggagtgtggc gaccactttg ggaggaatcc cctctttgcc aaaggtctgc tcaatcacaa
     1981 actgcattga cgcatcatcg aggggtcgca gtgtcagcaa cacataattt ccgctccgac
     2041 tcatctgccg aagccagccg gtaattggaa aatccaggga ggtggcttct cgctcgcgca
     2101 tggtggcaat cagaaacagc cggtgtccga tgctgttgcg catcagatag gccaaaaatt
     2161 ccagactcaa gccatcagcc cagtgcatat cgtcaatgac aaggaccact ggccgcaatc
     2221 gcgttacccc caggtacagc cgggttaaat agtcaaaaat ccgatatttt tcctggtcag
     2281 cactcaattg aaccgaagga gacgcaaacg tcagccattc gccttcggaa aaatcctgaa
     2341 tcacctgcgg cgtcatgggg ccaaacaacg ccggtaaccg gttttcagcc acttcgagaa
     2401 aatagccgat caccccccgg agtgattcat aaaaagcacg atatggcaat gaaccacccg
     2461 cttcataaaa ccgggtggtt aaaaaaagga catcttgccc ggccaggctc cattcaaatt
     2521 cctgaatcag ccgggttttt ccaattccag ccgcgccgct gacaaagacc ggatgtccgc
     2581 gtccaacttt gaccagatta aactcatgca gcaattgctc tgactcaagc gcccgaccaa
     2641 caaattgttc aaatcggagg tgacgtgacg ccgggtcttc aggccgatga ctggcaacct
     2701 gaaccgcctt ccggcctttt gccagcaagc caaacgcgtg gttactctcg gttggaaaaa
     2761 gcgtgacact ggttttcaag gaaatttcgg tgtcagactc ttcgacggca aagacctgag
     2821 ccgcacaggc ttcggtaaat tgagttgcgc tttcagccgc acttttcgta ccgctggcca
     2881 gaaacagaac tccaaattca ctccccccga tgcgtccaat cacggtggct ggacgcaaca
     2941 aaacgcgcat ccagtccgcc actttacgga gcatgcgatc
//
LOCUS       DAOQDO010000563         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26587_length_2979_cov_3.842339, whole genome shotgun sequence.
ACCESSION   DAOQDO010000563 DAOQDO010000000
VERSION     DAOQDO010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26587_length_2979_cov_3.842339"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PKZ53_10020"
     CDS             complement(<1..1018)
                     /locus_tag="PKZ53_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNJ40814.1"
                     /translation="MRKTVFSARRFVCLVLLGLLVVTPAFIQHIAVKADSRPGTEAQA
                     FIDEYTDTWLKLLYTYNLAEWESNTRIIEGDTTNAKATNAALEKLSAFCGSKGNIEKA
                     QAFLKQKDQLTPVQKLQLERILYLAADTPEIAKDVVSKRIAAQTTQVEKLYGFDYKID
                     GKSVTTNQIDDILRTSTSLTERRKAWEASKEVGKTLKDGLVNLRQLRNQSVKPLGYKS
                     YFDYQVSEYGMTSAEMMKLMDEINRQLRPLYRELHTYARYELAKKYNAPVPDTLPADW
                     LPNRWGQDWSSMVNVEGMDVDSALKDKSPEWIVKQAEQFYISLGWPALPPVFWEKSSL
                     YPPPP"
     gene            complement(1276..1446)
                     /locus_tag="PKZ53_10025"
     CDS             complement(1276..1446)
                     /locus_tag="PKZ53_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HNJ40815.1"
                     /translation="MWTLLGWLAIALSTVAVFDILGSSRTLGEKVMLIVFVVAFPLVG
                     STLYLTMLREKK"
     gene            complement(1665..>2979)
                     /locus_tag="PKZ53_10030"
     CDS             complement(1665..>2979)
                     /locus_tag="PKZ53_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40816.1"
                     /translation="TSPSRTFPQLSDILNNRATFGESLPTTSRTLNFRVTVRDNRATG
                     GGTNFDSTRVTVTSQSGPFLVREPNTAVQWVVGSTQNVVWDVASTSQSPVNCANVRIL
                     LSTDGGQSFPITLAAETPNDGTEAVTVPNNLTSTARIKVEAVGNIFFDISNANFQIAQ
                     SASNTPPTITAVPAITQAQGGSLTGVPIATVTDKETSVGNLIVQAIQIPAGLVISQIA
                     NSNGMITANLSTGCRATTGANNFTLQVTDSEGLKATARLTVNVTANLPPTIGEYQAAT
                     VTQNSSVTVSPSAPPVDDGSATVTATAEGFTGTFAADPQTGVVTVNNAGPVGTFTVTV
                     RIADTCGEAVTKTFSLTVNPAVQITSASYSKKLLSITGTGFGTSPTIIVNGTSLPASV
                     IQPGGTDISVTAKGGKKKLSLRKGENTIQVIGVGGVQSTAVTLTL"
BASE COUNT          725 a          706 c          766 g          782 t
ORIGIN      
        1 cgggcggagg cgggtagagc gacgattttt cccagaagac aggtggcaat gccggccagc
       61 caagactgat gtaaaactgc tcagcctgtt tgacaatcca ttccggagac ttgtctttga
      121 gtgctgaatc aacatccatg ccttcaacat tgaccattga actccagtcc tggccccaac
      181 gattggggag ccaatccgcc ggaagcgtgt caggaacagg cgcgttgtat tttttcgcca
      241 gctcataacg ggcataggtg tgaagttcgc gatagagtgg gcggagctgg cggttgattt
      301 catccatcag tttcatcatt tccgccgaag tcatcccata ttcagacacc tgatagtcaa
      361 aatatgattt atacccaagc ggtttgaccg actggttgcg gagttgtcgc aaattgacca
      421 gtccatcttt gagtgtttta ccaacttctt tggaggcttc ccaggcttta cgccgttcgg
      481 tcaggcttgt cgaagtgcgt aaaatatcgt caatttgatt tgtggtcacc gacttgccgt
      541 caattttgta atcaaagcca tacaatttct caacctgggt ggtttgagcg gcaattcgtt
      601 ttgaaacgac atctttggcg atttcgggag tatcagccgc cagatagaga atgcgttcca
      661 actgcaattt ttgaaccggc gtcaactggt ctttttgctt aaggaacgcc tgtgcttttt
      721 caatattgcc tttgctacca caaaaggctg agagtttttc aagcgccgca tttgtggctt
      781 tggcattggt tgtatcaccc tcgatgatgc gggtgttcga ttcccattcg gccagattat
      841 aggtatacaa cagcttgagc caggtgtcgg tatattcgtc aataaacgcc tgggcttcgg
      901 tccctgggcg actatctgct ttgactgcaa tatgttgaat gaatgctggc gttaccacca
      961 gcaatccgag cagcacaagg cagacaaatc gccgggcgga aaaaacggtt tttcgcatga
     1021 gtgagcagtc tcctgttttg gaaaattgtg atgagtgtca actgagatgg aaggaacggg
     1081 ctgtgggttg cgaaagcaca tcccgagttg gaggttatat caaactcaag actcaaaacc
     1141 cagtgtggga agctgctggg aaggcgtcaa ttcaggcagg ttcggggcaa aaaaaatgcc
     1201 cgctcaaaga ttgagcgggc caaatcacca gatgcaatgt gtgtacaatg aaaacgaatg
     1261 aaaggggtat aaaagctatt tcttttcgcg gagcatggtt aaatacagtg tcgagccaac
     1321 cagcgggaat gcaaccacaa acacaattaa catgactttt tcccccaggg tgcggctgct
     1381 acctaaaatg tcaaaaacgg caactgttga caaagcaatc gctaaccaac cgagcaatgt
     1441 ccacataatt gacaaccttt cgaacgggaa ttttgaagaa ggagtgaaat gactttggct
     1501 gatacgtgac tcaagcgaat gaagttcgca tcaatcttac aactttttta cttttgcctt
     1561 ctttgtatcg gtttcccatt tatcgagacc atcatttcag gtccattgcc cggcaggagt
     1621 atctctagca agctattgaa aagaaagagc ttcctcaatc atcttcataa ggtcagcgtc
     1681 acggcagttg attgaacccc gccaactcca atcacctgaa ttgtattttc acctttgcgc
     1741 aaactgagtt ttttctttcc acctttcgcc gtcacgctga tgtcagtccc accaggttga
     1801 atcacgctgg ctggcaaaga ggtgccattc acgataatcg ttggacttgt tccaaacccg
     1861 gtgccagtga tggaaagcag ttttttcgaa tagctggcac tggtaatttg caccgccggg
     1921 ttcacggtca aggagaaagt tttggtaaca gcttccccac aggtatcggc aattcgaacg
     1981 gttaccgtaa aggtgccaac tggtccggca ttgttgaccg ttacaacccc tgtctgcggg
     2041 tcagccgcaa aggttccagt gaatccttcc gccgtcgccg tgacggttgc cgaaccgtca
     2101 tccacgggag gcgctgacgg agaaaccgtc acggaactgt tttgcgtcac ggttgcagcc
     2161 tgatactcgc caatggttgg aggcagattg gcggttacat tgactgttaa cctggccgtt
     2221 gcttttaatc cttcagaatc agttacctga agcgtgaaat tgttggcacc agtggttgcc
     2281 cggcacccag ttgacagatt ggctgtgatc atcccattcg agttggcaat ctgcgaaatc
     2341 accagacctg ccggaatctg gattgcctgg acaatcaaat ttccgacaga tgtttcttta
     2401 tcggtgacag tggcaatggg aactccagtc aatgatccgc cttgtgcctg ggtaatcgcc
     2461 ggaaccgccg tgatggtcgg cggcgtgttg ctggcagact gagcaatttg gaaattggcg
     2521 tttgaaatat caaagaaaat atttcccacc gcttcaactt taatccgggc agtgctggtt
     2581 aaattgtttg gtacggtcac ggcttcggtt ccatcatttg gtgtttcagc cgccagggtg
     2641 attggaaatg attgaccacc atcggttgaa agcagaatcc gcacattggc acaattcact
     2701 ggtgattgtg aggtactggc cacatcccag acgacatttt gggttgaccc aaccacccat
     2761 tgaaccgccg tgttgggttc tctgaccaga aacggtccgc tttgactggt cactgtaacc
     2821 ctggttgaat cgaaatttgt gccgcctccg gtggcgcgat tgtcgcgaac ggtcacccga
     2881 aaattcaggg tgcggctggt ggtggggagt gattcgccga aggtcgcccg gttattcaaa
     2941 atgtctgaca gttgagggaa cgttcgggat ggactggtt
//
LOCUS       DAOQDO010000564         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26602_length_2978_cov_3.273349, whole genome shotgun sequence.
ACCESSION   DAOQDO010000564 DAOQDO010000000
VERSION     DAOQDO010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26602_length_2978_cov_3.273349"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKZ53_10035"
     CDS             complement(<1..288)
                     /locus_tag="PKZ53_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40817.1"
                     /translation="MSKRMIGICLTVLFLGIAGLLGVFESTVNAGTGSVVGPSSDDGL
                     RPGFKGIAQFRNEAQFYGSPRYGDSFERMAANNARVENQGRATGSAALSLIL"
     gene            complement(343..2118)
                     /gene="lnt"
                     /locus_tag="PKZ53_10040"
     CDS             complement(343..2118)
                     /gene="lnt"
                     /locus_tag="PKZ53_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNJ40818.1"
                     /translation="MPNLGTQLSGIQLRPYLLSALLFALSYPSFPWIRLEVLAWVWMV
                     PMLRALRSVESFWRFILLAGVTMLSGWTIAMSWLAWGNAVGAMAIVVLVSVVTTVPFA
                     VFYVIQKALGRRWALLTLPVVWTAWEWCYQNVDGMVAWPGIAVTQHKLTWLIQYIDIT
                     GMWGIVFWLVSLNVLIVMAVENRGQGTGDREQGIETEDQVPGLGREVAHSILHSSFSI
                     LHSQFFILNSSFSILHSSFIRRLGLIAVVMFGPPLLYAAFIFSQTDPSAGGQSIRVMV
                     VQPNIDPWQKSSPEIRQKVVGSLVDQTNQVLNTTTARPDLIVWPEVAVPVPLLQDEFA
                     REFVFRNVARWNIPVVTGTIDVRTVVPGNPQPTRIKASKPKNELFNAAVLLTPIQTEL
                     GHRQTVSSEPYRKRKLMPFTEEVPYAKRFPVLSKLAIDLNWNKEFNRGDRSTVFSFPT
                     HQGNQVTVATPICYEHFYPSLMAEFVRKGAQVITLLSNDAWFSKSHGEYQIAAFTTLR
                     AIETRRSIVRCTNTGLTCFIDRFGRISQTIPWWQEDVAVADIALSNQTSLYVRFPDAF
                     PKLCFCGVIVLGLAAGLNRMSFLNS"
     gene            complement(2364..>2978)
                     /locus_tag="PKZ53_10045"
     CDS             complement(2364..>2978)
                     /locus_tag="PKZ53_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40819.1"
                     /translation="VRTAVLENPALPWEEIQARINYLPESVLAQLARHPAIPGTFLQV
                     LAENPGRILRGAVTRHSQTPLSMLESWMDGETWLRAELAHNPKLPAVWLEKLAQDASA
                     AVRVGVACNPATPAGLLRQLACDPDRSVRLGVAKNHQSPGDVLEVLFQADPGNANLFE
                     ALLAHPHLPGSVLEDMATRWPRNRQIARHPNATSKLKEKILGKS"
BASE COUNT          812 a          788 c          726 g          652 t
ORIGIN      
        1 caatatcagg gacaacgcgg ctgaaccagt tgcccgcccc tggttttcaa ctcgcgcatt
       61 gttggccgcc atccgttcaa atgaatcgcc ataccgggga ctgccataaa attgagcttc
      121 atttcggaac tgggcaatcc ccttaaatcc tggccttaaa ccatcgtctg aagagggacc
      181 tacaacacta ccagttcctg cattcactgt ggattcaaat actcccaaca acccggcaat
      241 tcctaaaaac agaacggtta aacagatccc aatcattctt ttcgacatac tgtagctcct
      301 tcatcaatag ctggtagaaa aactatgcgc ataccaacgg aatcaactgt tcaggaaact
      361 catccggttt aatccggcag ccaatccgag aactatcact ccacaaaaac ataattttgg
      421 aaaagcatct ggaaaacgaa catacaggct cgtctggttg gaaagtgcaa tatcggcaac
      481 ggcaacatct tcctgccacc aggggattgt ttgagagatt cgcccgaagc gatcaataaa
      541 acaggtcaga cctgtgttgg tgcagcgaac gattgaccga cgggtttcaa tcgctctcag
      601 cgtcgtaaac gccgcaatct ggtattcacc gtgcgatttt gaaaaccagg catcgttact
      661 gagcagtgtg atgacctggg caccctttcg gacaaactcc gccatcaacg acgggtagaa
      721 atgttcataa caaatcggag tagccaccgt gacctggttc ccctggtggg tggggaagga
      781 aaacactgtc gagcggtcgc ctcggttgaa ttccttgttc cagttcaggt caatggccag
      841 tttggaaagc accggaaagc gtttggcata gggcacttcc tcggtaaagg gcatcaattt
      901 gcgtttgcgg tacggttcgg atgaaaccgt ttgccgatgc cccaactcag tttgaatcgg
      961 agtcaacagc accgccgcat taaataactc attctttggc ttcgaagctt ttatccgggt
     1021 gggttggggg ttaccaggaa ccaccgtccg gacatcaatg gtgccagtca ccaccgggat
     1081 attccatcga gccacattgc ggaagacaaa ttctcgggcg aattcgtctt gaaggagcgg
     1141 cactggaacc gccacctctg gccagacaat caaatcgggc ctcgctgttg tggtattcag
     1201 cacctgattg gtttgatcca caagactgcc cacaactttt tgacgaatct ctggtgaaga
     1261 tttttgccag ggatcaatat ttggctgaac caccatcact cgtattgact ggcctccagc
     1321 cgatgggtcc gtctgtgaaa aaataaaagc ggcatagagc aacggcgggc caaacatcac
     1381 gacggcgatc aaacccaacc gccgaatgaa tgaagaatga agaattgaga atgaagaatt
     1441 gagaatgaag aattgagaat gaagaattga gaatgaagaa tgaagaattg agtgagcaac
     1501 ttccctgccc aaacccggaa cttgatcttc agtttctata ccctgttccc tgtcccctgt
     1561 cccctgtccc ctgttttcaa ccgccatcac aatcaacaca ttcagcgaca ccagccagaa
     1621 gacaatgccc cacattccag tgatatcaat gtactgaatc agccacgtca gcttgtgctg
     1681 ggtgacagca attccgggcc aggcgaccat cccgtcaacg ttttgatagc accattccca
     1741 ggctgtccag accactggca acgtcaggag cgcccatcgt cggccaagtg ctttttgaat
     1801 cacataaaaa acggcaaacg gcaccgttgt gaccacactc accagaacga caatcgccat
     1861 tgcaccaaca gcatttcccc aggctaacca ggacatggca atcgtccagc cactcagcat
     1921 ggttactcca gccagcaaga taaaccgcca gaacgactca accgaccgga gcgcccgcaa
     1981 catcggcacc atccagaccc aggccagaac ttcaaggcga atccacggaa atgaaggata
     2041 agaaagcgca aagagcagcg cactcaaaag atatgggcgc agttgtattc cagatagctg
     2101 agttcccaga tttggcatga acgtatcgcc tccagaatga accccggcag tcacagtaaa
     2161 agaaaagaaa tgttttggtt tttggaagaa tacaaaggaa gggctgggtc aatacccagg
     2221 ccagaatggt ttacaacctc tcttattggg gtaaacaaga aaagacctgg aaaagggtca
     2281 gggtgtggaa aatatttttt tgtagctgcc gcggtcgatg aagatattga gcatgaaaaa
     2341 ggcagaagaa gttgagaggg tattcaactt tttcccaata tcttttcctt gagtttgctg
     2401 gtggcatttg gatgccgggc aatctgacgg tttcgcggcc accgggttgc catatcttcc
     2461 agtacagatc caggtaaatg gggatgagcc agcaaagcct caaacaggtt ggcattgccc
     2521 ggatccgctt gaaacaatac ctccaggacg tcacccggtg actggtgatt ttttgcaacc
     2581 ccaagccgaa cgcttcggtc tgggtcacaa gccagttggc gcaataatcc agccggggtc
     2641 gccggattgc aggcaactcc aacccgaacg gctgccgagg catcctgagc cagtttttca
     2701 agccacacgg ctggtagctt tgggttgtgc gccagttcag ctcgaagcca cgtctcgcca
     2761 tccatccagg attcgagcat tgaaagcggg gtttgcgaat gcctggtgac ggcgccacgg
     2821 agtatccgac caggattttc cgccagcacc tgcaaaaacg ttcccggtat ggctggatgt
     2881 ctggcaagct gcgccaatac tgattctggc aaataattga tccgagcctg gatttcttcc
     2941 caggggagtg ccggattctc aagcaccgcc gtgcgaac
//
LOCUS       DAOQDO010000565         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26613_length_2977_cov_3.351129, whole genome shotgun sequence.
ACCESSION   DAOQDO010000565 DAOQDO010000000
VERSION     DAOQDO010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26613_length_2977_cov_3.351129"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1757
                     /locus_tag="PKZ53_10050"
     CDS             279..1757
                     /locus_tag="PKZ53_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40820.1"
                     /translation="MKPDNQFATRKRKEIEPLLTDVVRAERNIERWPAIWTPANSRNT
                     AKVRILERQKFLEDGSRLACQVKITPLIEYGNLTTEDQKVFLGLIKLWEEAYKPEELN
                     FSLRQLAKCLDIASWSGEVGETLKKSLWKLRGTLFIWTNSYCDGVTKEFHKHLNSFTI
                     LSELEIFEKETAGSTNKQACRLKFYPLIEKNLRNRYTQPTFYNVVIGFQSGLAQLLYQ
                     YLELTMVDKTNFERTSKNLFEELAIEGKKYQYKSARKRALESAIRELDNVPIPTGVLR
                     VSLEETKKNDDWKLVIKKIAQMLLPVGQADKEAEAEPHITDLSDFPNKEAQNTRHQPI
                     EHQDQATELVQHFHKTFFSLDVEEPGAHELKQAADLIKKHGFEVAKYVVNYAYRQSQE
                     THFNIATFGGILHYVGRAKARYKKDQQAQERIRQTEERERSFETCPLGCWRNGGRVFW
                     VKIDDQGGSINQMDCLHDEVRHKEMEHKDKIKIAMTCADLKS"
     gene            1934..2698
                     /locus_tag="PKZ53_10055"
     CDS             1934..2698
                     /locus_tag="PKZ53_10055"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ40821.1"
                     /translation="MKLAVLNQKGGVGKTTVSVNLAYGFAHEGKRTLLVDLDPQAHST
                     IIYSSPPKKPEATVKDLLLSKTSDIRKAIIPASVNGQPVNGLFLIASNIHLAITAEQI
                     IASTHREKLLHNHFKKIEKDFDFIVMDCPPTLNVLTVNAIYTADLILIPTTYSVNALD
                     GIADLFKSVSDVKESEDFAFLIVRNGYDARNKDSNRFIEENLEPYRQYVLKTVIRRIE
                     AINQAPMNREPIFTFDPTSRGTEDFQALTSELIVYA"
     gene            2691..>2977
                     /locus_tag="PKZ53_10060"
     CDS             2691..>2977
                     /locus_tag="PKZ53_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40822.1"
                     /translation="MPKKKITGASKLLTEKEPSAPPTAPLNLTTPKVKKPRLKNFRLN
                     DVDQEQLRRIVDEVNRLSPYKKISEATIIKSLIFLGSKMHPEKILKATKEV"
BASE COUNT          956 a          646 c          635 g          740 t
ORIGIN      
        1 atttgccggt tttaaaccag cgccaagctg tggtgtatga aatcccttgt tgttttgccc
       61 aatctgataa tttcataata ggcaatagta taatatattt gcctataaat caagtgaatt
      121 attagcagac attctcagac gtgtgtgtgt attatgaaaa accacatagt cttgctattt
      181 tacctggcca aacccaaaaa ttttgagcgg tgaggaaaga aaaaacgcct gcttgattac
      241 aactcggcgc gtcatatact gtttaagatg gactgattat gaaacccgac aatcaatttg
      301 cgactcgcaa acgaaaagag attgaaccgc ttctaaccga tgttgttcgt gcagaacgaa
      361 acattgagcg atggcctgca atctggacac ctgcaaattc aagaaacacg gccaaagtca
      421 gaatcctaga acgtcagaaa ttccttgaag atggcagtag acttgcctgc caggtcaaaa
      481 taacaccact tatcgagtac ggaaatttaa caaccgaaga ccagaaggtg tttcttggtc
      541 tcataaagct atgggaagaa gcctataaac ctgaagagct caatttctcg cttcgtcagc
      601 ttgccaagtg tttagatatt gcatcctgga gtggtgaagt tggagaaact ctgaaaaaat
      661 cactctggaa acttcgcgga acactcttta tctggacgaa ttcgtattgt gatggcgtaa
      721 caaaagagtt ccacaaacac ttgaactcat tcacgatcct gtctgaattg gaaatctttg
      781 aaaaagaaac ggctggaagt accaataaac aagcctgtcg cctcaagttt tatccattga
      841 tcgagaaaaa cctgagaaac cgctataccc aaccgacctt ctacaatgtg gtgataggtt
      901 ttcaaagcgg ccttgctcaa ctcctgtacc agtaccttga gctaacgatg gtggacaaaa
      961 ccaattttga gcgtaccagt aagaatcttt ttgaagaact ggcgatagaa ggcaagaaat
     1021 accaatataa atctgcaaga aaaagagctt tggaaagtgc tatccgtgaa ctagataatg
     1081 ttcctattcc tactggagtt ttgcgcgttt cccttgagga aaccaaaaaa aatgatgatt
     1141 ggaaattagt tatcaaaaag attgcacaga tgttattgcc agttggccag gcagataagg
     1201 aagcagaggc cgagccacac atcaccgatc tttcagattt tccgaataag gaagctcaaa
     1261 acaccagaca ccagccgatt gaacatcagg atcaagctac cgaacttgtc cagcacttcc
     1321 acaaaacgtt tttcagtttg gatgttgaag aaccgggagc acacgagctg aagcaagccg
     1381 ccgacctcat caaaaagcac ggcttcgaag ttgctaagta cgtggtgaac tatgcctacc
     1441 gacaatccca ggaaacccat ttcaacatcg caacctttgg cggcatcctt cactatgtgg
     1501 gcagagcaaa agcccggtac aagaaagacc aacaagcaca ggaaaggatt cggcagacag
     1561 aggaacgaga acggtcgttt gaaacttgcc cacttggatg ctggagaaat ggcggtaggg
     1621 ttttttgggt gaaaatagac gaccaggggg gctccattaa tcaaatggac tgtctacacg
     1681 atgaagtcag gcacaaagaa atggaacata aagataagat aaaaattgca atgacctgtg
     1741 cagatctgaa aagctaaact gttgaaaata caaatctatt gttgtgaaaa ataagtcata
     1801 ggtgctttat aaattgcttt cgtattctct ttctacaata ctttctattg atctttacaa
     1861 aatagcttga ttttacccat atataaacta tagtgagatc gtagattctt ctcttcactc
     1921 tatgggtagc tgtatgaaac tggcggtact gaatcaaaaa ggcggagtgg gcaaaacaac
     1981 ggtttccgtc aaccttgctt atggttttgc tcacgaagga aaacggacac ttttggttga
     2041 tcttgaccct caagcacatt caaccatcat ttattcctca ccacctaaga agccagaggc
     2101 aacggtgaaa gacttgcttt tgagcaagac aagtgacatc aggaaagcaa tcattcccgc
     2161 cagtgtgaac ggtcaacctg tcaatggtct atttttgata gcatccaaca ttcatcttgc
     2221 tattaccgca gagcagatca tagccagcac ccaccgtgaa aaattgctcc acaatcattt
     2281 taagaaaatc gaaaaagatt ttgattttat cgtgatggat tgcccaccca cgttgaacgt
     2341 gttgaccgtc aatgcgatct acacggcaga cctgatcttg attccgacca cttacagtgt
     2401 gaacgccctg gatggcatcg ctgacctttt taaatcagtg agcgatgtca aagagagtga
     2461 ggattttgct tttttgattg tgcgcaatgg gtacgacgct cgcaacaagg acagcaacag
     2521 gtttatagag gagaacctgg agccctatcg ccagtatgtt ttgaaaacgg ttatccgaag
     2581 aattgaagca atcaatcaag cgccaatgaa ccgagaacca atctttacct ttgatccgac
     2641 ttccagagga acagaggatt ttcaagccct tacatctgaa ttgatagtct atgcctaaaa
     2701 agaagattac tggagcgagc aaacttttga ccgaaaagga gccgagcgca ccgccgaccg
     2761 cgccgctcaa tctgactacc cccaaggtga aaaagccccg cctgaagaat tttcgtctca
     2821 atgatgttga ccaagaacag ctcaggcgaa ttgtggacga ggtaaaccgt ctcagcccgt
     2881 acaagaaaat tagtgaagcc accatcatca agtctctaat ttttttaggc agcaagatgc
     2941 accctgagaa aatcctaaag gctaccaaag aagttta
//
LOCUS       DAOQDO010000566         2976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26640_length_2976_cov_2.597398, whole genome shotgun sequence.
ACCESSION   DAOQDO010000566 DAOQDO010000000
VERSION     DAOQDO010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26640_length_2976_cov_2.597398"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1355
                     /locus_tag="PKZ53_10065"
     CDS             93..1355
                     /locus_tag="PKZ53_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNJ40823.1"
                     /translation="MLIRRLLQVMSLIGWLLLSGAFVCQAHESKPPAKIPETIRIGLF
                     TLFQPRTLSIKPILSPAEIFAGQVRRQLQPAKTYRIRLGNGQLALFEGQIKILEGTSL
                     ELATTGSVPAAQSPAELELIAQSSTRRVAGKILIQVRENALQVILTQSLEDAVAVVTA
                     SEMKGVGLSEALKCQAIVVRSFFMTHLGRHQRLGYDVCDSTHCQLYFGTLFDQSYPPR
                     LIQSARQATHATRATVLWEAEHPVEAYYTACCGGHTTVSSVVWPLNDEIEPKQDVPTV
                     ICNWCRGANFYRWQRRIGVHRLLSTMTSIWGAKPLDKVDLQISKRTPDGIVTHLALSD
                     GQRTIEVANGEFRSVIGHTLGWNLVLSNVYQITRRGDFYEFTGHGFGHQVGLCVEGTV
                     AQAKAGRSYQEILRFYFPDATLNDRVTK"
     gene            1458..2879
                     /locus_tag="PKZ53_10070"
     CDS             1458..2879
                     /locus_tag="PKZ53_10070"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNJ40824.1"
                     /translation="MSTNGKELSFFENVISYFDQAASFTQHPKTLLEQIKTCNSVYSF
                     EFPIRSQDGIKSITAWRVEHSHHKLPVKGGIRYSIHANEDEVKALAALMTYKCAVVNV
                     PFGGAKGAIQINPKDFSTAELEQVTRRYTAELIKKNFIGPGIDVPAPDYGTGSREMAW
                     IADTYAAFHPGHIDAHACVTGKSISQGGIRGRFEATGRGVFYSLREACAHKDDMEALG
                     LTPGIEGKRVVVQGLGNVGYHSAKFCQEGGAVLVGLAEYEGAIHNPAGLDLEAVVKHR
                     RETGSILNFPGATNIATAHEALELDCDILIPAALENQITEANAPRIKAKIIAEGANGP
                     TTAAAEAILYSRGALIIPDIYCNAGGVVVSYFEWLKNLSHVRFGKMDKRFDQMVFGNI
                     LTTIERTTGKSLSDAEKTAIAHGADEIELVNSGLEEIMAIAYNEIHTIWKTRPGVTTL
                     RSAAFISAIDKIAISYAELGIFP"
BASE COUNT          754 a          706 c          779 g          737 t
ORIGIN      
        1 agttcaatca aataaactta gtgaactact gactactggc tactgactac aaactggtat
       61 tacattgttt gggaatctga ggcgttcaaa gaatgctgat acgtcgtttg cttcaggtta
      121 tgagcttgat cggctggttg ttgctctcgg gtgcttttgt ttgtcaggca cacgagtcaa
      181 aaccacctgc caaaatccca gaaacgattc gaattgggct ctttacacta tttcaaccgc
      241 gaacactctc aattaaaccg attctctctc cggctgagat atttgctggc caggtccgcc
      301 gccagcttca gcctgcaaag acctatcgca ttcgactggg aaacggacaa ctggcactgt
      361 ttgaggggca gatcaaaatt ctggaaggaa caagcctgga gcttgcaacc actggatctg
      421 tacccgctgc ccagtcgccg gctgaacttg agctaattgc tcagtcgagc acccggcgtg
      481 tcgccggaaa aattttgatt caagtcagag aaaatgccct gcaagtcatt ttgactcaat
      541 cccttgaaga tgcggtggct gtggtgactg cctcagagat gaaaggggtt gggttgagtg
      601 aagccctcaa gtgtcaggcc atcgtggtgc gaagtttttt tatgacacat cttggacgcc
      661 accaaagatt ggggtacgat gtgtgcgatt caacccattg ccagttgtac ttcggtacac
      721 tctttgacca atcctatccg ccgcgcctga ttcagtctgc ccgtcaagcc acacacgcca
      781 cacgtgccac ggtgctctgg gaagccgagc atccagtcga agcctattac actgcttgtt
      841 gtggtggcca taccaccgtt tcatcagtcg tctggccatt gaatgatgaa attgagccca
      901 aacaggatgt gccgacagtt atttgcaact ggtgtcgcgg ggcaaacttt taccgatggc
      961 aacgccgcat tggggtgcat cggctgttaa gtacaatgac atcaatatgg ggagctaaac
     1021 cattagataa agtggatttg cagatttcaa agcgcactcc tgatggtatt gtaactcacc
     1081 tcgccctctc tgacggccag cgaacaattg aggtcgctaa tggtgaattt cgaagcgtga
     1141 ttggtcacac gctgggttgg aatctggttt tgagcaatgt ctatcaaata actcgacgcg
     1201 gtgactttta tgaatttacg ggtcatgggt ttgggcatca ggttggtttg tgtgtcgaag
     1261 gaactgttgc tcaagccaaa gcgggtcgat cttaccagga aatcctgcgg ttttacttcc
     1321 cagacgccac gttgaatgac agggtgacaa agtgacaaag tgatatttct tcgaaaccct
     1381 gaaccctgaa ccctgaaatt ggcagtatcc cctcgcaatt gcgatcaacc tacgttttat
     1441 cacaggagga atcccccttg agtaccaacg gaaaagaact cagtttcttt gagaacgtga
     1501 tttcttattt tgatcaggca gcgtcattta cccaacatcc caaaacactg cttgagcaaa
     1561 ttaaaacttg taacagtgtg tattcctttg aatttccaat tcgttcgcag gatggaatca
     1621 aaagcattac agcctggcgt gtggagcata gccaccacaa actgccagta aagggtggta
     1681 ttcggtattc gatacatgct aacgaagatg aggtcaaagc gctggcagca ctgatgacct
     1741 acaagtgtgc agtggtcaac gtgccatttg gcggtgccaa aggtgctatc caaatcaatc
     1801 caaaggattt ttcaacggct gaactcgaac aggtcacccg tcgctacacc gcagaactga
     1861 tcaagaagaa ttttattggt cccggaattg atgtaccggc ccccgactat ggtacgggct
     1921 cccgagaaat ggcctggatt gccgatacct atgcagcatt ccaccctggc cacattgatg
     1981 ctcacgcctg tgttaccggc aagtccattt cgcagggagg gatccgggga cggtttgaag
     2041 ctactgggcg tggggtgttt tattctctgc gcgaagcctg tgcccataaa gacgatatgg
     2101 aagccctggg gttgactccg gggattgaag ggaaacgcgt tgtggtgcag gggcttggca
     2161 acgtgggcta ccattcggcc aaattctgtc aggaaggtgg tgccgtgctg gttggtctgg
     2221 ctgaatacga aggggccatc cacaatccag ccgggttgga tctggaagct gtggtcaaac
     2281 accgtcgtga aaccgggtca attttgaact tcccaggggc aaccaatatt gcaaccgctc
     2341 acgaagccct cgaacttgac tgcgatattc tgattccggc ggcgcttgaa aaccagatta
     2401 cagaagccaa tgcaccgcgc attaaagcca aaattattgc cgaaggggcc aatgggccaa
     2461 ccacggcggc ggctgaagcc attttgtaca gccgtggggc tttgatcatt ccggatatct
     2521 actgcaatgc gggtggtgtg gtggtgtctt actttgaatg gttaaagaac ctgtcgcacg
     2581 ttcggtttgg gaagatggac aagcggtttg atcaaatggt gtttggaaac atcctgacaa
     2641 ccattgagcg caccaccggc aaatcattgt ccgatgctga aaaaaccgcg attgcccacg
     2701 gcgccgatga aatcgaactc gtcaactctg gtctggaaga aattatggcg attgcctata
     2761 acgaaattca taccatctgg aaaacacggc ctggagttac aacgttgcga tccgccgcgt
     2821 ttatcagtgc cattgataag attgctattt cgtatgctga actcgggatt ttcccctaaa
     2881 acatcaggct gaagaagttg ggctgaagaa gttgggctga agaagttggg ctgaagacaa
     2941 cgggctgaag acaacgggct gaagaagtcg ggctga
//
LOCUS       DAOQDO010000567         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26671_length_2974_cov_2.741350, whole genome shotgun sequence.
ACCESSION   DAOQDO010000567 DAOQDO010000000
VERSION     DAOQDO010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26671_length_2974_cov_2.741350"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKZ53_10075"
     CDS             complement(<1..830)
                     /locus_tag="PKZ53_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40825.1"
                     /translation="MTYYKYPVLVWQDYQGMYTGRFVDQEIGSQMIAATGLTVESVLA
                     ELKELIQWQAAEWHQSIYPNFESAQLITFRVEVRPEHQFKNRRFPASETVALRAACVY
                     GEQHSGLLVAALPMLGIQFYYYQPGELKNLVLAYVQESFKGASTRQIVSLLDPKSLQL
                     LELSVNVGKVKYARPKVYSATQTLEMVAEPLGDPALRRQFGRAYERETEIQLLVKKLT
                     EETPNLILLGESGSGKSTVLVEAVRQAELYLKKAEAETDRSRPPRRYWLTSGSRLIAG
                     "
     gene            complement(872..1858)
                     /locus_tag="PKZ53_10080"
     CDS             complement(872..1858)
                     /locus_tag="PKZ53_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ40826.1"
                     /translation="MARDLEGRLNEREIFETEYQVLDEAESALKNEELSRDDLRKAFK
                     TLAKKYKSLLSQTVKITRIGDSTQSKLIKIQNQLDQQKSQLEAANLKLRELDQIKQNF
                     TAMLVHDLKSPLSVVKGTLEFLSMDESLEETGYGELIRASEGSVQKILNLINEVLEVY
                     KSEAQDMKLVLTPQNPQALIRECIVEAEVAGRAAGLTVVPNIVDDLPTISMDAGKLGR
                     VFSNLLSNAVKFTPKGGHIVVEARTTAGTGIEAGSKFLTVEITDTGEGIPAEELPFLF
                     DPYRQAKSTKAKLGVGLGLAIVKRIVAAHGGNIVVRSKVGVGTCFSVVIPAV"
     gene            2046..2966
                     /locus_tag="PKZ53_10085"
     CDS             2046..2966
                     /locus_tag="PKZ53_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ40827.1"
                     /translation="MKHCLLFLGLVVALSLTVVAVPRQSEKPSSVQELVAAEKAFAKY
                     SVENGMREAFIEFFTDEGINFVPQPVLTREFLKKRPPTPKPLPFILDWTPMTGDIARS
                     GDLGYNTGPFMVTVPSQKDQKPSFGYFFSIWKKQADGTWKVVVDAGIQSEEAGKRPLS
                     EVGYQQTPIKTVELPTKSIISEKEKEAFLVADKALTQATQTKGRAESYTAVLLPKSRL
                     HLNDLAPLTNRDQILAYLKDHPASMTGTVTHSDMSKAADFGYTVGSYELKPQAGDKTE
                     KGYYVRIWRQVAKKDWKLAFETFLPLPPEK"
BASE COUNT          730 a          771 c          730 g          743 t
ORIGIN      
        1 atgccagcaa tcagccgcga gccactggtc aaccagtatc gccgtggagg ccgtgatcta
       61 tcggtttcgg cttctgcttt tttcagatag agttcggcct gccggaccgc ctcgaccagg
      121 acggttgatt ttccgctgcc gctttcccca agcaaaatca ggttgggtgt ttcttcagtc
      181 agttttttga ccagaagttg aatttcggtc tcacgttcat acgcacggcc aaactggcgc
      241 cgcaatgcgg ggtctccgag cggttcggcg accatttcca gtgtctgcgt cgcactatag
      301 acttttggtc tggcatattt caccttaccg acattgactg ataactcaag cagttgcaat
      361 gatttcggat ccaggagcga tacaatttgg cgggtcgagg cgcctttaaa tgattcctga
      421 acataggcca ggaccagatt ctttaattca cccggctgat agtaataaaa ctggataccc
      481 agcattggca gtgccgccac cagaagccca ctatgttgtt caccataaac gcaggctgcc
      541 cggagcgcca cggtctcaga ggctggaaag cgccggttct tgaattgatg ttcaggtcgg
      601 acttcaaccc ggaacgtaat aagctgggct gattcaaaat ttggataaat actctggtgc
      661 cattctgctg cctgccactg gatgagttct ttgagttcgg ccaaaactga ttccaccgtc
      721 agcccagttg ccgcaatcat ttgcgatccg atttcctgat caacaaaccg gccagtgtac
      781 attccttgat aatcttgcca gaccaaaacc gggtatttgt agtacgtcat agaatacaaa
      841 agcggcgtca agccgcagca ctccgaaatt tctaaaccgc cggaatcacc actgaaaaac
      901 acgtgccgac gccaactttg ctccggacga caatatttcc gccgtgggca gccacaattc
      961 gtttcacaat cgccagtcct aacccaacgc caagcttggc cttagtggat tttgcctggc
     1021 ggtagggatc aaacaaaaat ggaagttctt cagccgggat gccttcgccg gtatcggtaa
     1081 tttcgacggt caggaacttt gagccggctt cgatgcccgt gccggcggtg gtgcgggctt
     1141 caaccacaat atgaccgcct ttgggggtaa atttcacggc attggagagc agattcgaaa
     1201 agacccgccc cagtttgccc gcatccattg aaatcgtggg caggtcatcc acaatgttgg
     1261 gcacaacggt taatccagcc gcccggcccg caacttcagc ttccacaata cattcgcgaa
     1321 tcagcgcctg agggttttga ggggtaagca ccagtttcat gtcctgggct tcgcttttgt
     1381 agacttcgag cacttcgttg atcagattca gaattttttg aacactgccc tccgatgccc
     1441 gaatgagttc gccatacccg gtttcttcca gcgattcatc catgctcagg aattcgagcg
     1501 tgcctttgac cacactgagc ggcgatttca agtcgtgaac cagcatcgcg gtaaagtttt
     1561 gcttgatttg atccagttcg cgcaatttca gattggcggc ttcgagctgg ctcttttgct
     1621 ggtccaactg attttggatt ttgatcaact tgctctgggt tgaatctcca atccgggtga
     1681 ttttgactgt ttgcgaaagc aggcttttgt actttttcgc cagtgtttta aaggctttgc
     1741 gcaaatcatc acgtgacagc tcttcatttt tcaatgctga ttcggcttca tccagaacct
     1801 ggtattcagt ctcaaaaatc tcgcgttcgt tgagtcttcc ttcaaggtcg cgtgccatgg
     1861 ccaaaagttc tcccggatca aacaggccgg cgcacaaggc tgagggtgcg ccggcgaaat
     1921 aaaatgaacc acagtggggt tggagggcgg tgaaacggtt gaatcaatca aaaggcgtgg
     1981 gtattgtagg gcgctcaaga aaaattgcca gtccatgccg acaaattccc tcaggaaaga
     2041 catctatgaa acactgtttg ctctttttag ggctggtggt tgccctttct ttgactgtcg
     2101 tggctgtgcc gcgccaatcg gaaaaaccat cttcggttca ggaactggtt gcggctgaaa
     2161 aagcttttgc caaatattcg gttgaaaatg ggatgcgcga agccttcatc gaatttttta
     2221 ctgatgaagg aattaacttc gtcccccaac cggttttaac ccgcgaattt ctcaagaaac
     2281 gccctccgac accgaaacca cttccattta ttctggattg gacgccgatg acgggtgaca
     2341 tcgcccgttc cggcgacctg ggctataaca ccgggccatt tatggtcacg gttcccagcc
     2401 agaaagatca aaaaccgtca tttggctatt ttttctcaat ctggaaaaaa caggcggatg
     2461 gtacctggaa agttgtggtt gatgcgggga ttcaatctga ggaagctggg aaacgcccat
     2521 tgtccgaggt tggctatcag caaacaccga tcaaaaccgt ggaactccca accaaatcca
     2581 tcatttctga gaaagaaaaa gaagcgttcc tggtggcgga taaagccttg acacaggcca
     2641 cccaaaccaa aggccgggca gaaagctata cagcggtgct gcttccaaaa tcgcggcttc
     2701 acctcaatga cctggcacca ctgacgaacc gtgatcaaat tcttgcctat ttgaaggacc
     2761 atccggcgtc aatgactggg acagtgaccc attccgacat gtcaaaagcg gctgattttg
     2821 ggtacaccgt cggcagttat gaacttaaac cgcaagccgg agataaaacc gaaaaagggt
     2881 actatgtccg gatctggcga caagtcgcca aaaaagactg gaagctggca tttgaaacct
     2941 tcctgccgct tccacctgaa aaataaaatt tgtc
//
LOCUS       DAOQDO010000568         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26686_length_2973_cov_3.361892, whole genome shotgun sequence.
ACCESSION   DAOQDO010000568 DAOQDO010000000
VERSION     DAOQDO010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26686_length_2973_cov_3.361892"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PKZ53_10090"
     CDS             complement(<1..950)
                     /locus_tag="PKZ53_10090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNJ40828.1"
                     /translation="MAKNAPNPNPMLDRSLPSSIEAERGVIASILIDNALCYQAIELL
                     KPEEFFRDSHRRIYEKMIVLAEQRMPIDPVTLQSALARAGELEQVGGLAFLTSFLDGV
                     GHSSNVESYARIIKGKAMLRRMISVCQQGIASCLDQEDDPESIVDQIEKQIFDIGEDR
                     IRAGFVSVAEVAGPELQRIEDASKRSALLTGLMTNFVEFDRLTNGLQNSDLVIIAARP
                     SMGKTSFCLNLAENIAIQGSKSVGIFSLEMSKESLVTRMLCSQGRVDAQRMRGGYLNK
                     DEWKALGEALQVLAQARIFIDDAPGITVMEMRAKARRLMS"
     gene            complement(1000..1137)
                     /locus_tag="PKZ53_10095"
     CDS             complement(1000..1137)
                     /locus_tag="PKZ53_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40829.1"
                     /translation="MLPVPGNVSLLLFLARLITVCFGGGFEQDRKPVALALQVAHPNV
                     T"
     gene            complement(1184..1669)
                     /gene="rplI"
                     /locus_tag="PKZ53_10100"
     CDS             complement(1184..1669)
                     /gene="rplI"
                     /locus_tag="PKZ53_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNJ40830.1"
                     /translation="MATMELLLKVDVENLGTRGQIVRVRAGYGRNYLLPRSLAVEATP
                     TNVNLIERQRQHLLKLAAQELATAKTSADQLTSTTLTFARKVGAHGLLYGSVTAMDIA
                     EALKAQGTEIERRKILLKDPIKEPGEYEVAVKLHAEVKPLLKIVVVPEETSTTPEAAA
                     K"
     gene            complement(1908..2315)
                     /gene="rpsF"
                     /locus_tag="PKZ53_10105"
     CDS             complement(1908..2315)
                     /gene="rpsF"
                     /locus_tag="PKZ53_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNJ40831.1"
                     /translation="MSSNRIFEVMFILFPGLEEEEIDKIINQLTHVISDKGGKVAKTD
                     KMGRRRLAYEIRKGNQTFREGYYVLLTIEGSGAEIAETERRLRVLDPVIRYLTVRIDE
                     DLKRAKKVQDHRSSKTTRRSQGGDDADLDDANA"
     gene            complement(2484..>2973)
                     /gene="pth"
                     /locus_tag="PKZ53_10110"
     CDS             complement(2484..>2973)
                     /gene="pth"
                     /locus_tag="PKZ53_10110"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNJ40832.1"
                     /translation="VKLNECEALTGRITLAGEPVLLVKPQTYMNLSGQSVAQLCTKYQ
                     ADPADDLMVVVDDISLPFGKLRLRPKGSAGGHNGLKSLIARLHTQEFSRLRVGILPEH
                     PVGNLADFVLAAFTSREREGLEDILSQSCRAVELWVAQGIEPAMAQFNGQGIEPAPQS
                     GK"
BASE COUNT          729 a          792 c          711 g          741 t
ORIGIN      
        1 tgggacatca accgtcgggc tttagcgcgc atttccatga cggtaatgcc tggcgcatcg
       61 tcaataaaaa tccgagcttg agccagaacc tgcagcgcct cgcccagcgc tttccattca
      121 tctttgttga gatatccgcc gcgcatccgc tgggcgtcaa cccggccctg ggaacacaac
      181 atgcgcgtga caagtgattc tttcgacatt tcaagcgaga aaattccaac cgatttgctg
      241 ccttgaatgg caatgttttc agccagattc aaacaaaaac tggtttttcc catcgagggt
      301 cgggcggcaa tgatgaccag atccgagttt tgcaacccat tggtcaatcg gtcaaattcc
      361 acaaaattgg tcatcaaccc ggttaacaaa gccgagcgtt ttgaagcgtc ttcgatgcgt
      421 tgcaactcag gtccagcgac ttctgccacc gagacaaacc cggcacgaat ccggtcttcc
      481 ccaatgtcaa aaatctgctt ttcaatttga tcaacgatgc tttcagggtc atcttcctgg
      541 tccagacacg aagcgatccc ctgctggcaa acactgatca tccgacgcag catggccttg
      601 cctttgataa tccgggcata ggattcgaca ttggaagaat gcccgacacc gtccagaaaa
      661 ctcgtaagaa aggccaggcc gccaacctgc tccagttcgc ccgcccgggc aagtgcggat
      721 tggagtgtga ccgggtcaat cggcattcgc tgctcagcca gcacaatcat tttttcatag
      781 atgcggcggt gactatcacg aaagaactcc tctggtttga gaagttcaat tgcctgataa
      841 cacagggcat tatcaatcaa gattgatgcg atcacacccc gttcggcttc aatacttgaa
      901 ggcaaagaac ggtcaagcat tggattcgga ttgggcgcat ttttggccat gagactacct
      961 gtaactctat gattggggaa agtggattaa agaccggcat caggtcacgt tggggtgtgc
     1021 aacctgaaga gcaagtgcca cgggtttgcg atcttgctca aagcctccac caaaacacac
     1081 tgtaatgagc cgtgccagaa acagcaagag cgagacgttg cctgggacag gcaacatttc
     1141 gctctcaaaa gatctatcga gttttcaaca ggttaaagtc aacctatttt gcggctgctt
     1201 ctggtgttgt ggacgtttct tctggaacaa ccacaatttt gagcaacggt ttgacttcag
     1261 cgtggagttt gaccgccact tcatattcac ccggttcttt gatcgggtct ttcaacagga
     1321 ttttccgacg ttcaatttcc gtgccctgag ctttgagcgc ttcagcaata tccatggcgg
     1381 tcactgaacc atagagcagg ccgtgagcac cgactttccg ggcaaaagtc aacgtggtgc
     1441 tggttaactg gtcggctgag gtcttggcag tggccagttc ctgagcggca agtttcagca
     1501 aatgctgccg ttgacgttca atgaggttga cgttggtcgg cgtggcttcg accgccaggc
     1561 tgcgaggcag gaggtagttc cgcccatacc cggcacggac gcgcacgatc tgaccgcggg
     1621 ttccaaggtt ttcaacatcg actttcagta acaattccat cgtagccatg acacatcacc
     1681 cttacgttca gagcttctcg cttctgagag aattcggtca accagattga ccgacaacca
     1741 ggaaattcac cgccagccca gagcacgtgt ttgcaatcac gaagtatgaa tgatgaaaac
     1801 gcgatgccgg ctgaatatca ggcaggtcat tgcattctgg ttcttcattc ttcatttttc
     1861 attcccatgc ctgcggcggg aaccaacttt gccgcatcag cgcagcatta cgcatttgca
     1921 tcatcgaggt cagcatcatc gccaccctgt gatcggcggg tggtttttga cgaacgatga
     1981 tcctgaactt ttttggctcg tttcaggtct tcatcaatgc gaacggtcag gtagcgaatc
     2041 accggatcca acacacgcaa ccgacgctcg gtttccgcaa tttcagcccc gctgccttcg
     2101 atagtcagca gcacgtaata cccttcgcgg aaggtctgat ttcccttgcg aatttcatat
     2161 gccagccggc ggcggcccat tttgtcggtt ttggcgactt tcccgccttt atcactgatc
     2221 acgtgggtca actggttgat aattttatca atttcttcct cttccaaacc tggaaaaagg
     2281 ataaacatca cttcaaaaat tcggttcgaa ctcacaacaa aaactccttc tggatttagc
     2341 tgtaacagcc aaagcctccg tctgacgaac atcgttcgca aaaaacggaa gcaagggagg
     2401 gagcaaaatt gaataaagaa ttaagaatta agaattaaga attcaatcca ctggggttgt
     2461 tcattcttca ttcttcattc gcttcacttc cctgattgag gagcaggttc gattccctgc
     2521 ccattaaact gtgccattgc tggctcgatg ccttgagcca cccagagttc aacagcccga
     2581 caactttgac tgaggatgtc ttccagccct tcgcgctcgc gactggtaaa agccgccagg
     2641 acaaaatcag ccagattccc aactggatgt tcaggtaaaa tacctacccg taagcgtgaa
     2701 aactcttgag tgtgcaatct cgcaatgagt gatttcagac cgttgtgtcc accggcgctg
     2761 ccttttggcc gcagtcggag tttgccaaac ggaagtgaaa tatcatcaac cactaccatt
     2821 aaatcgtcag ccgggtctgc ctgatatttg gtgcagagtt gcgccaccga ctgcccgctc
     2881 aaattcatgt aggtctgggg ttttaccagt aaaactggct cgccggccag ggtaatgcgt
     2941 ccggtaaggg cttcacattc gttcagtttc acg
//
LOCUS       DAOQDO010000569         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26770_length_2967_cov_2.854739, whole genome shotgun sequence.
ACCESSION   DAOQDO010000569 DAOQDO010000000
VERSION     DAOQDO010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26770_length_2967_cov_2.854739"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKZ53_10115"
     CDS             <1..402
                     /locus_tag="PKZ53_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40833.1"
                     /translation="AGAAACVMTAGTGYFGHRVAADVGERSAYMYHNYTVALSDLGSM
                     ASDVARIDDLVRSARSSQDAAEVRSLQARSDKLAEKVERAIIDKNARIGNNVQLLNDK
                     GIQEATAENYVIRDGIIIIPKGAIVPDGTVV"
     gene            729..1910
                     /locus_tag="PKZ53_10120"
     CDS             729..1910
                     /locus_tag="PKZ53_10120"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNJ40834.1"
                     /translation="MSYPVILSAVRLPIGKFQGSLKSFRAPELGALAVRAAIERAGID
                     AAQVDEVVMGNVLQAGLGQNPARQAALGAGCPTTVAALTVNQVCGSGLRSVMLAAQSI
                     QTGDAQFVVAGGMESMTNAPYLLQRARDGYRLGHGEMLDSMIQDGLWCAFDNWHMGNT
                     GEYVAEHYNVTREDQDNFAAGSHAKAVAAVNAGKFTTEIQAVSIQQKKGDPIVFSTDE
                     GPRADTTASSLVKLRPAFKKDGTVTAGNASSINDGAAAVVVTSEQQAAALGRAPMARI
                     VAQAMSGVDPKLVMMAPVEAVKKVIVKAGWKLDDVDLFELNEAFSAQAVAVTREIGAD
                     PARVNVNGGAVALGHPIGASGARVLVTLLHALKDRGAKRGVASLCLGGGNAVALAVEC
                     L"
     gene            2159..2812
                     /locus_tag="PKZ53_10125"
     CDS             2159..2812
                     /locus_tag="PKZ53_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNJ40835.1"
                     /translation="MSSVHFHRTTHHKLFWFIVGGLFFLSSACTTSKHPTMNPTRTEL
                     SAAEIRLREAARQAHVSYPLQNPEIKIFKGARRIELWAEKKLVKAYKIGLGTSPVEDK
                     EREGDFRTPEGKFYVCTRNEKSKFHLFLGVSYPNQEDAQRGLASGLITKEQYDAIVDA
                     QNKQERPPWNTPLGGEVGIHGHGSNSDWTWGCIAVENQEIEELWLACPMKTPITIAR"
     gene            2871..>2967
                     /locus_tag="PKZ53_10130"
     CDS             2871..>2967
                     /locus_tag="PKZ53_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014274975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ40836.1"
                     /translation="MSTVIHPQPQIEIVYPESDGKPMADNTEQFEW"
BASE COUNT          672 a          808 c          816 g          671 t
ORIGIN      
        1 gctggcgcag cagcgtgtgt catgaccgcc gggaccggct atttcgggca ccgggtcgcg
       61 gccgacgtcg gcgagcgttc cgcgtacatg taccacaact acaccgtggc gctcagcgac
      121 ctcggctcca tggccagcga cgtggcgcgc atcgatgacc tcgtgcgcag cgcgcggtcg
      181 agccaggacg ccgccgaggt gcgctcgctc caggctcgca gcgacaagct cgcggagaag
      241 gtcgagcgcg ccatcatcga caaaaatgcc cgcattggaa acaatgtcca gttactcaat
      301 gacaaaggca ttcaggaagc cacggctgaa aattacgtca tccgggatgg catcatcatc
      361 atcccgaaag gcgctattgt tccggatggg acagtggtgt agggttggca cgtggtgctt
      421 ggtgcttcgt tcttggtgct ttgtttttgg tgcttcgtgc ttggtgcttc gttcttggtg
      481 ctttgttctt ggtgctttgt tcttggtgct tcgtgcttgg tgcttggtgc ttggtgcttc
      541 gttcttggaa agcattggtt cctaagtacc aggtaccaag taccaagcac caagcaccaa
      601 gtaccaggta ccaggtacca ggtaccaagc accaagtaca aagtaccaag caccaagcac
      661 caagtaccaa gtacaaagtg ttaagtactc atccctgtca tacatcatcc agaggtacca
      721 acatttttat gagttatcca gtcattctta gtgcagttcg cttgccaatc ggcaagtttc
      781 aaggaagtct taaatcattt cgtgcgcccg aattaggcgc cctggcggtt cgtgccgcca
      841 ttgaacgggc cggcattgat gccgcccagg ttgacgaagt cgttatgggc aatgtgctcc
      901 aggcgggttt gggtcagaac ccggcccgtc aggcagcact tggcgccgga tgtccaacca
      961 cggttgccgc gttaaccgtc aatcaggtct gcggttccgg gttgcgttcg gtcatgcttg
     1021 ctgcccagtc catccaaacc ggagacgccc agtttgtggt tgccggcggg atggaatcca
     1081 tgaccaatgc cccgtatttg ctccagcgag cccgcgatgg ctatcgtttg ggacacggtg
     1141 aaatgcttga ttccatgatt caggatggat tgtggtgtgc ctttgacaac tggcacatgg
     1201 gcaataccgg tgaatatgtc gccgaacatt acaacgtcac ccgtgaagac caggacaatt
     1261 ttgccgctgg ttcacacgcc aaagcggttg ccgctgtcaa cgctgggaag ttcacaaccg
     1321 aaattcaagc cgtctcaatc cagcagaaaa aaggcgatcc gattgtgttt agcactgatg
     1381 agggcccacg ggctgacacc actgccagca gtctggtcaa actgagaccg gccttcaaga
     1441 aagatggcac agttactgcc ggcaatgcct caagcatcaa cgacggcgcc gccgccgtgg
     1501 ttgttacgtc agaacaacag gctgccgcac tcggtcgagc cccgatggcc cgcattgtgg
     1561 ctcaagcgat gagcggtgta gatcccaaac tggtcatgat ggccccggtc gaagccgtca
     1621 agaaagtcat tgtcaaagcc ggatggaagc tcgatgacgt ggatttattt gaactcaacg
     1681 aagcgttttc agctcaggcc gtggctgtca cccgtgaaat cggcgctgat ccagccaggg
     1741 tcaacgtcaa tggcggtgcc gtggccctgg gtcacccaat tggtgccagc ggtgcccgcg
     1801 tcctggtcac gctcttgcac gcactgaaag accgtggtgc caaacgtggt gtcgcttccc
     1861 tctgccttgg cggaggcaat gcggtcgccc tcgcagttga atgcctttaa aacctcaggg
     1921 cttggggctg aagaaaacgg gctgaagaag acgggctgaa gaagacgggc ttggggctga
     1981 agaaaacggg cttaggatat gaaacccgtt ttcttcagcc ctgagccccg agcttgcgag
     2041 tcttcacccc gatgtcttca gcccgatgtc ttcagccccg agccccgagc ttgcgagtct
     2101 tcagcccgaa gttttcagcc ccaggccctg agccccggtt ttttcagccc caggccctat
     2161 gtcttcagtt cactttcatc gaaccaccca ccataaattg ttctggttta tcgtgggtgg
     2221 tttgtttttt ctctcctcgg cctgtaccac gtcaaagcat ccgaccatga accccactcg
     2281 aaccgagctt tccgctgctg aaattcggct ccgggaagcc gcccgtcagg ctcacgtttc
     2341 ttacccgctt caaaatcctg aaatcaaaat ctttaaaggt gctcgccgga ttgaactctg
     2401 ggctgagaaa aagcttgtca aagcgtacaa aattggactg ggaacgtctc cggttgaaga
     2461 caaagagcgg gaaggcgatt tccgaacccc ggaaggcaag ttttatgtct gcacccgcaa
     2521 cgaaaaaagc aaattccatt tatttctggg cgtgagttat cccaaccagg aagatgctca
     2581 gcgcggtttg gcttcagggt tgattacaaa agaacagtac gacgccattg ttgatgccca
     2641 gaataaacag gaacgccccc cgtggaacac accgttaggt ggcgaggtcg gtatccacgg
     2701 tcatgggtcg aactctgact ggacctgggg ctgtattgcg gttgaaaatc aagagatcga
     2761 agagttgtgg cttgcctgcc caatgaaaac cccgatcacg attgccaggt aatacacaag
     2821 tctttaaact tatagcctgt tcaatatcca gttgggagag gaattctggt atgtccacgg
     2881 tgattcatcc acagcctcaa attgaaatag tctatccgga aagcgatggg aaacccatgg
     2941 cagacaatac cgaacaattc gaatgga
//
LOCUS       DAOQDO010000570         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26787_length_2966_cov_3.162487, whole genome shotgun sequence.
ACCESSION   DAOQDO010000570 DAOQDO010000000
VERSION     DAOQDO010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26787_length_2966_cov_3.162487"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2809
                     /locus_tag="PKZ53_10135"
     CDS             <1..2809
                     /locus_tag="PKZ53_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ40837.1"
                     /translation="EDGEYRIPALDVGTYDISIKVEGFAPQTVRTELLIGRTSIVNFA
                     LTVGSSGGEIINVTADAASIVETTRTQQSANVNDRAIRELPVNGRNFLEFVRLTPGVN
                     TDPRGGDISFGGLRGTANSLLIDGADNNNTFFGQTIGRTGSGRGPYQFSQSSVREFQV
                     NTNSFSAEFGRAGGAVINVVTKSGTNEFHGDANIYFRDRSLNAEDAIAKAARRAKPRN
                     RIYQFGATLGGPIAKDRAFFFFNYDGQRNTEPQPVILGVPVPNDPVSQAVANQLRAFT
                     GTYNRQFNQDVFMGKVDWRINDKNQYSVRYNRQNFTGTNLENSGPQSAFEHTGNSLVT
                     TDTLTNQFTTIVKPNLINELRVLFSRDKEPGQSNSTAPEADIRQGGVPILALGRNNFS
                     PRETTEKRFQIIDTMSYLRGAHNMKFGVDVVINRIFNFFPGLFTGVYSFTSYADFADG
                     KPAGGYRQAFAGDGTNGPVSRPNSNEFAAFFQDDWSVTPRLKLYLGVRYDVQRKAEPP
                     VANADPQLAALNISTQGINQDTNNFAPRLGFAWQPFDDGGKTVIRGGYGIFYGRTPSI
                     MLGTGHTQNGISVRTLNFFGDNVPTYPGNFTTPPTTGVPATPALFFFAKDYVDPMIHQ
                     GSFGIERELTKDLSLSVSWLTVRGMRQSRSRDINFSSPVSQLVNVRTSTAINFANGQP
                     LGTLTGTLFIPRYGLRPATRFDRLTQFESTGDSIYHGLVVALNKRFSQNFQGLISYTW
                     SHAIDNKPDQTSVVVGADDAKNLEDPLNFGNERGDGESDTRHRFVLSYSWDLAYARNL
                     SNPVAKAILDGWSLSGIVTATAGRPYSATVGGDLNGDRNARTDRAPGTSRNVFTTDKF
                     VNVDMRLTRTINLTEKYRLQFFAEGFNLFNRFNVGTVNTNQFLFAVIQGVPTIAPNRD
                     FGAPLTSLGARQFQLAARFVF"
BASE COUNT          703 a          836 c          737 g          690 t
ORIGIN      
        1 cgaagatggg gagtatcgga tcccggcact ggatgtcgga acctatgata tctcaatcaa
       61 agtggagggc tttgcacctc aaaccgtccg aactgagttg ttgattggaa gaacttccat
      121 cgtcaacttt gcattgacag ttggttcatc aggtggagaa atcatcaatg ttaccgctga
      181 tgcggcgtcc attgttgaaa ccacccgtac ccagcaatca gccaacgtca atgatcgtgc
      241 cattcgcgaa ttaccagtca atggccgtaa ctttctggaa ttcgtacggt taactccggg
      301 cgtcaacacc gatccacgcg gcggtgatat ttcctttgga ggcttgcgcg gaacggccaa
      361 cagcctgctc attgatggtg ctgacaacaa caacaccttc tttggacaga cgattggacg
      421 aaccgggtct ggacgtggtc cataccagtt tagccaaagc tcggtgcgtg aatttcaggt
      481 caacaccaac tccttctcgg ctgaatttgg ccgggctggg ggggcggtga tcaatgtggt
      541 gaccaaatcg ggcaccaatg aatttcacgg cgatgccaat atctactttc gtgatcggtc
      601 actcaacgct gaagacgcaa ttgcaaaagc agctcgccgc gccaagccac gcaaccggat
      661 ttatcaattt ggggcgacgc tgggtgggcc gattgccaaa gatcgcgcct ttttcttctt
      721 taactatgac ggacaacgca acaccgaacc acaacccgtg attctgggag ttccagtccc
      781 aaatgacccg gtttcccagg cggtggccaa tcagttgcgc gccttcaccg gcacctataa
      841 ccggcaattt aaccaggatg tgtttatggg taaagttgac tggcggatta atgacaagaa
      901 ccagtactcg gttcgttata accgtcagaa ctttaccggg accaacctgg aaaactcagg
      961 cccacagagt gcctttgaac acaccggtaa ctcgctggtg acaactgata ccttaaccaa
     1021 tcagttcacc actatcgtca agccaaacct gatcaatgaa ttacgggtgc tcttctcccg
     1081 tgataaagag ccaggtcaat ctaacagcac cgcacctgaa gccgatatcc gccagggtgg
     1141 cgttccgatt ctggcactcg gacgcaataa cttcagtccg cgcgaaacca ctgaaaaacg
     1201 gtttcagatt attgacacca tgtcatacct gcgtggcgct cataatatga aatttggggt
     1261 ggatgtggtc atcaaccgga ttttcaactt cttccctggc ctgttcaccg gggtgtatag
     1321 ctttaccagc tatgccgact ttgctgacgg caagccagca ggcgggtacc gtcaggcatt
     1381 tgccggtgat ggaaccaacg gcccggtttc acgcccaaac agcaatgaat ttgcggcctt
     1441 cttccaggat gactggtcag ttaccccacg cttgaagttg tatctgggtg tccgctatga
     1501 cgttcagcgc aaagctgaac cacccgtggc caatgcagat ccacaactgg cggcactcaa
     1561 catttcaacc cagggaatta accaggatac caataacttt gccccacgcc ttggctttgc
     1621 ctggcaaccg tttgacgatg gtggcaaaac ggtcatccgt ggtgggtacg gtattttcta
     1681 tggccgcacc ccatccatta tgctcggcac tggccatact caaaatggga tcagtgttcg
     1741 gacattgaat ttctttggcg ataacgtccc cacctatcca gggaacttca ccaccccacc
     1801 aaccactggg gtgccggcca caccggcgtt gttcttcttt gccaaagact atgttgaccc
     1861 gatgatccat cagggcagct ttgggattga gcgtgaactg accaaggacc tgtccttgtc
     1921 agtttcctgg ttgacagttc gtggcatgcg ccaatctcgg agccgggata tcaactttag
     1981 ttcgccagtc tcacaactgg tcaacgtccg aaccagcacc gcaatcaatt ttgccaacgg
     2041 tcagccgttg ggaaccttga ccggaacgct gtttattcca cggtatgggc tccgtccagc
     2101 cacccggttt gatcgcctga cccagtttga aagcactggt gattcgatct accacggcct
     2161 ggtggtcgcc ctcaacaaac ggtttagcca gaacttccag ggattgattt cctacacctg
     2221 gtcccatgcg attgacaaca aaccagatca aacctcagtt gttgtcggag ccgatgacgc
     2281 caagaatctg gaagacccac tcaacttcgg caatgaacgc ggcgatgggg aatcagacac
     2341 ccgccaccgg tttgtgttga gttattcgtg ggatctggcc tatgcccgca acctcagcaa
     2401 cccagttgcc aaagccatcc tcgatggctg gtcactgagc ggaatcgtga cggccactgc
     2461 cggacggcca tattcggcca ctgtcggcgg cgacctcaat ggtgaccgca atgcccgaac
     2521 tgaccgtgca cctggcacca gccgcaacgt gttcacaact gataaatttg tgaatgttga
     2581 tatgcgcctg acccgcacga tcaatctgac cgaaaaatac cgcctccagt tctttgctga
     2641 aggctttaac ctcttcaacc ggtttaacgt cggaacggtc aataccaacc agttcctgtt
     2701 tgccgtgatt caaggtgttc cgaccatcgc gccaaaccgc gatttcggag ccccactcac
     2761 cagcctcggc gctcgccagt tccagttggc tgcccggttt gtgttttaag atctggggct
     2821 gaaaatactc gggctgaaga aaccgggctt ggggctgaag acatcgggct gaagaaaatg
     2881 ggttgaagaa tcaaggttct gacccacctt cttcagcccg agtattttca gcccgatgtc
     2941 ttcagcccga gtattttcag cccgag
//
LOCUS       DAOQDO010000571         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26817_length_2964_cov_2.352011, whole genome shotgun sequence.
ACCESSION   DAOQDO010000571 DAOQDO010000000
VERSION     DAOQDO010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26817_length_2964_cov_2.352011"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..421)
                     /locus_tag="PKZ53_10140"
     CDS             complement(8..421)
                     /locus_tag="PKZ53_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40838.1"
                     /translation="MYTFADSKPQTDQHNWARSWIDFCTFFVLTDLALAVLYPVHAGY
                     LAHSLNQLTGLKFAEFIDWPYADLSVCRFGWATPFGILFLANLLPAFVLSCLSLIFIV
                     NSIQSARKKPLFLDHHPGKYHSQTQLNSSQNVCVA"
     gene            complement(425..2017)
                     /locus_tag="PKZ53_10145"
     CDS             complement(425..2017)
                     /locus_tag="PKZ53_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ40839.1"
                     /translation="MPNFQIKAEKQPTRCEICHQADQFDPKTSTCRRCLENVVVINVP
                     TRPVVGSILPLTRTERGWLVVGGFVVGDSLMTLILPFRGGVITTAACQFFGLTGVVEI
                     NALTPSDCGMFGIFREYPIFFNHITLSLFLIWCLAFFIEQAAGKSPTRLFVYPPIKDL
                     IFSQSFRLKLWKYWYPKFAVFALGILINLVVFSGFVLPKYTLADAVRNDYQFLAKALR
                     AKGDSIDQVDHHQWTPLMYACANGDLAMVHDLLDHGANVNAQSIEGQTPLMCAVIPGY
                     AEITELLLQRGAHINHQDEWGRSALMHALEKYRSNIGHFRLDPDNTRALQNGVGSSID
                     QTIQVLLAYIPDLVGPKDVGGQTIAMKAASSGNTEILTLARSQISWELDEKDHSGKTV
                     LMYAVEGNNPEVVREVLSYQHNIDAQDNFGMTALMYALSNEHQHALIFFEMLIEAGAN
                     IHVKDSMGHTALVYAIQNKQLEVTRWLIEVESDLNLQSKIDLSLVGLIQAHFSRKQAD
                     ELVSLINQKQQAVTSQSAPSRK"
     gene            complement(2027..>2964)
                     /locus_tag="PKZ53_10150"
     CDS             complement(2027..>2964)
                     /locus_tag="PKZ53_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ40840.1"
                     /translation="LVHFQPLFTTAMMYAIADGNTAMVNELISRGANVNTTNHLGQTP
                     LMCAAWAGKPDLVKRLLGHGAQVNAQDHQGKSALLTVLTTRSIDRVLDRLDGSDSFDH
                     LYFSHPDEFLQKETLEILLDCPESRLNLTDFQGSTLTSYAVRTGNLELVQRVLSKLPN
                     QLDQPDKAGTTPLMLACKNKLPKLVEYLLGQGASPLHKDRSGRTALFYAAHVQFYNQH
                     ASVQFIDQLIAQGAEINVQDIEGKTPLMEALDQGNHEMVRTLLAHNPNIQVRDNRGLR
                     ALEHFTLRTAQFSGKAYRGLYQRLSVVENKPQPKH"
BASE COUNT          795 a          685 c          721 g          763 t
ORIGIN      
        1 ggaaatctca ggccacgcac acattttgag aagagttgag ctgagtttga gaatggtact
       61 tgcctgggtg atggtccaaa aacagaggtt tttttcgggc tgattgaatg gagttgacga
      121 taaaaatgag ggagagacag ctcaacacaa aggcaggaag caggtttgcc agaaataaga
      181 tcccaaatgg agttgcccac ccaaagcggc agacactgag atctgcatag ggccaatcaa
      241 taaattcagc aaatttcagc cctgtgagct gattcaaact gtgagccaaa taaccagcat
      301 gaactggata gagcaccgcc agagccagat ccgtcaagac aaagaaggta cagaaatcaa
      361 tccagcttct cgcccaattg tgctgatcgg tttgaggttt tgaatcggca aacgtgtaca
      421 tatcctattt tctggaaggc gctgattgag aagtaacggc ctgttgtttc tggttgatga
      481 gcgacaccag ttcatcggcc tgttttcgag aaaaatgtgc ctggattaac ccaactagac
      541 tcaggtcgat ctttgactga aggttgagat cagactctac ttcaatgagc catcgagtta
      601 cttcaagttg tttattttga atggcataga ccagagctgt atggcccatg gagtctttta
      661 catggatatt tgcaccggct tcaatgagca tttcaaagaa gatcaatgcg tgttgatgtt
      721 cgtttgaaag ggcatacatt agcgccgtca tcccaaaatt atcttgcgca tcaatgttgt
      781 gttgatatga aagcacttcg cgtaccactt cggggttgtt tccttcaacg gcatacatca
      841 gaaccgtctt tccactgtga tctttttcat ccaattccca acttatttgt gatctcgcta
      901 acgtcaaaat ttcagtgttg cctgagctgg cggctttcat ggcaattgtt tgcccgccaa
      961 catctttcgg cccaactaaa tcagggatat aggccagtaa aacctgaatc gtttgatcta
     1021 tggatgaacc aacgccgttt tgtaaggctc tggtgttatc tggatccagc ctgaaatgcc
     1081 caatattaga ccggtatttt tccagggcat gcatcagcgc cgatctcccc cattcatcct
     1141 gatggttgat gtgagcacct cgttgaagca gtaattctgt aatttctgca tatcctggaa
     1201 taactgcaca catcagcggt gtttggcctt caatactttg ggcattgacg ttggcgccgt
     1261 gatcgagcaa gtcgtgaacc atggccagat caccgttcgc acaagcatac atcaagggtg
     1321 tccattggtg atgatccacc tgatcaatgg aatctccctt tgctcgtaaa gctttggcta
     1381 agaactgata atcatttcgg acagcgtctg ccaaggtgta tttgggaagg acaaacccac
     1441 tgaagaccac aagattgatc agtatcccga gtgcgaacac cgcaaacttg ggataccagt
     1501 atttccacag cttcaatcga aaggattgag agaaaatcag gtctttaatt gggggataga
     1561 caaataatcg agttgggctt ttacctgctg cctgttcaat gaaaaatgcc agacaccaaa
     1621 tcagaaagag ggaaagcgtt atgtggttga aaaaaattgg gtactctcga aagatgccaa
     1681 acatgccaca atcactcggg gtcagtgcat taatttcaac aactccggtc aatccaaaaa
     1741 attgacaggc cgcagttgtg atcactccac cccgaaatgg aagaatcaga gtcatcaacg
     1801 aatcaccaac cacaaaccca ccgaccacaa gccagcctct ttcagttctg gttaatggaa
     1861 gaatgctccc cacaactggc cgagtcggta cgttgatcac aaccacattt tctaaacacc
     1921 gccggcaggt actggtcttt gggtcaaact ggtcggcctg atgacaaatc tcacatcggg
     1981 tcggttgttt ttcagcttta atctgaaaat tcggcatcgt gacctcttaa tgtttgggct
     2041 gcggtttgtt ttcaaccaca gaaagtctct ggtatagccc tcgatacgct ttgcctgaaa
     2101 actgggctgt gcgcaaggtg aagtgttcga gcgcccgaag tccacggtta tctcgaacct
     2161 gaatattcgg gttatgagcc agcaacgtgc gaaccatctc gtgattgccc tggtcaagtg
     2221 cttccatcaa tggagttttg ccctcaatat cctgaacatt gatttcagcc ccctgtgcaa
     2281 tcagttgatc aatgaactga accgatgcat gttggttgta aaactggaca tgcgctgcat
     2341 aaaacagggc tgtccggcca gaccggtctt tgtgaagtgg tgatgctccc tggcctaata
     2401 aatattcaac cagtttgggc agtttatttt tacaggcaag catgagcggt gtggtaccag
     2461 ccttatccgg ttgatcaagc tggttgggaa gtttggagag tactctttga acaagctcca
     2521 gattgccggt ccgcaccgca taactggtga gggtggagcc ttgaaaatca gtgagattga
     2581 gtcgggattc tggacaatca aggagtatct caagtgtttc cttctgcaga aattcgtccg
     2641 ggtgagaaaa ataaagatga tcaaaggaat cagacccatc gagccggtcg aggacccggt
     2701 caatggaccg ggtagtcaag acagtcagta acgctgattt tccctgatgg tcctgggcgt
     2761 tcacctgggc accgtgcccc aggagccgct tcaccagatc gggtttaccg gcccaggcgg
     2821 cacacatcag cggagtttgg cccaggtgat tggtggtgtt gacattcgct ccgcgagaaa
     2881 tgagttcatt gaccatggca gtatttccgt cagcaatggc atacatcatc gccgtcgtga
     2941 acaaaggctg aaagtgaacc agcg
//
LOCUS       DAOQDO010000572         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26857_length_2961_cov_3.144873, whole genome shotgun sequence.
ACCESSION   DAOQDO010000572 DAOQDO010000000
VERSION     DAOQDO010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26857_length_2961_cov_3.144873"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKZ53_10155"
     CDS             complement(<1..512)
                     /locus_tag="PKZ53_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ40841.1"
                     /translation="MGFHLRQNLRFAFRMLIKMPGFSVITLLTLALGMGANTAIFSVL
                     NSVVLRPLPYQAPEHLFELTERSNQLGELSVSYPNFLDWQARSQSFQSLAAHRFDNFK
                     LSDGQIPERVNGRQVSGQFFETLGVKPLLGRLISAVDDQRTSEPATVISYGLWQRRFA
                     AEPGVLGRVI"
     gene            947..2389
                     /locus_tag="PKZ53_10160"
     CDS             947..2389
                     /locus_tag="PKZ53_10160"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ40842.1"
                     /translation="MAKLQIATKKALYLLVLLFILLPGLPLSAFPRFQKPPTDFGELE
                     RVILAELQEKNCPGAALAIVKGDQLIYSRGFGVANIETQTPVTPDTLFQIGSITKTFT
                     AVGVLGLVTDGKLKLDVPIGKYVQGLNPKLAQVTLHQLLSHTAGIIDEPDEFGSQDES
                     LMSGYLRSWNDDYALFPRGELFSYSNSGFALAGFVAAEALGTRYTGLMAERVFQPLGM
                     TRTTFQPTVAMTYPLAVGHQTQDKKPVVVRPLAHDARLYPAGTFYTSAHDMARFVTAI
                     LNGGKLDSKQVLAPSIFPQMQSPVARLLSTTDGTSYGYGLFMNQYRGVQQYWHEGSMT
                     GYVAMMAVFPEQKVGYILLCNGNGVDLSKSKEKIMEVMLPLKSKLINTPAPSQPLTQE
                     EMNFYAGQYFQPNRFRIEILIKNQQLFIKELGQEMPLTKIGEHRFSFQFPLAPQPWEI
                     YIQPGKDGKPLAAHQYVWGFKKIAPASTNQ"
     gene            complement(2625..>2961)
                     /locus_tag="PKZ53_10165"
     CDS             complement(2625..>2961)
                     /locus_tag="PKZ53_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ40843.1"
                     /translation="QLDLWGTKLVTLSACETGIGEAKNGDGVYGLRRALVLAGSEAQL
                     MSLWSVSDQATRKLMVDYYTRLKAGEGRSEALRNVQLEMLKDPRRQHPFYWAAFIQSG
                     EWANLDGKR"
BASE COUNT          753 a          766 c          704 g          738 t
ORIGIN      
        1 ttgatgaccc gccctaagac accaggttct gcggcaaacc ggcgttgcca taacccgtag
       61 ctgataacgg ttgctggctc gctggtgcgt tgatcatcaa ccgccgagat caaccggcca
      121 agcagcggtt tgacacccag tgtttcaaaa aattgaccag aaacctgtct tccgttcacc
      181 cgttcgggta tttgcccgtc tgagagctta aaattgtcaa accggtgtgc tgccagactt
      241 tgaaatgatt gagaccgggc ctgccagtcg agaaagttcg gatacgaaac cgataattcg
      301 ccaagctgat ttgaacgctc agtcaattca aagagatgtt ctggggcctg atagggcaat
      361 ggccgcaaga ctacgctgtt taagacactg aaaatggcag tgttcgcccc cattcccagc
      421 gccagggtca gcaaggtgat aaccgaaaag cccggcattt tgatcaacat gcggaaggca
      481 aagcgaaggt tttgtcgaag atggaatccc atagcggtgt gtttcctttt tcagaattgc
      541 gacccgattt ggacaaggaa gatcagaagg tcagatcaac ccttcacatg ccaagtcgcg
      601 tgccacaaac cggatctgat accagtttgg gctgagggta tcgggctaag cgctgaggga
      661 aatacaatat cttcaatatt ttagcttttc ataaggcgag gcttcattca aaaataggat
      721 gagccggctc ccggtccgag gccgaggttc cagatcatgc cggaactctg gctgagacgt
      781 gttgttcgaa attgggaagg tgacttccca caagtgaact caaaaccggg gtcgaatgaa
      841 caagaagatc acagcctgaa attctgcgtc tgaaaaactt gcattcccca gaatagtgtc
      901 tgagagtgtg ccaccccgga attccacctg aatggataaa atctttatgg ccaaacttca
      961 aattgcaacg aaaaaagcac tctacctact ggtactcttg ttcattcttc ttcctggtct
     1021 tccactttca gcctttccta gatttcaaaa accacctaca gattttggtg aactggagcg
     1081 agtgatcctg gctgaactcc aggagaaaaa ttgccctggt gccgctctgg cgattgtcaa
     1141 aggtgatcaa ctcatatatt ccagaggttt tggcgtggca aacatcgaaa cccaaacccc
     1201 agttacaccc gacaccttgt ttcagattgg gtcaatcacg aaaactttta cggcggttgg
     1261 ggttcttggt cttgtcactg atggcaagct caaactggat gtcccgattg gaaaatatgt
     1321 tcagggactc aatcccaaac tcgctcaagt gaccttacat cagcttttga gccataccgc
     1381 tggaatcatt gatgagccgg atgagtttgg cagtcaggat gaaagtctga tgagtggcta
     1441 tctgcgttcc tggaatgacg actatgccct gtttcctcgc ggagaattgt tttcctactc
     1501 aaattccggt tttgcactgg ctggatttgt ggctgccgaa gcgctgggaa cacgctatac
     1561 ggggttgatg gcagagcggg tatttcagcc gcttggaatg acccgcacaa ccttccagcc
     1621 caccgtcgcc atgacctatc cgctggctgt cggccatcag actcaagaca aaaaaccggt
     1681 cgtggtcaga ccccttgcac atgacgcacg gctctatcca gcagggacgt tttacaccag
     1741 tgctcatgat atggcccggt ttgtgacggc cattttgaat ggaggaaaac tcgattcaaa
     1801 acaggttctt gccccgtcaa tcttccccca aatgcaatca cccgttgcgc ggcttttgag
     1861 cacaactgat ggtaccagtt atggatacgg tctgtttatg aatcagtatc gaggtgtaca
     1921 gcagtactgg cacgagggct caatgaccgg gtatgttgcc atgatggctg tctttccaga
     1981 acaaaaagtt gggtacattc ttttatgtaa tggaaatgga gttgatcttt caaagagcaa
     2041 ggaaaaaatc atggaagtga tgcttccgct caaatccaag ctcatcaata ctccagcccc
     2101 atctcaacct ctgacccagg aagagatgaa cttctatgca ggtcagtatt tccagccaaa
     2161 ccgctttcgg attgaaattc tgatcaaaaa tcagcagttg ttcatcaaag aacttggtca
     2221 ggaaatgccg ctgaccaaaa tcggggagca tcggttttct tttcaatttc cgttggctcc
     2281 tcaaccgtgg gaaatctaca ttcagcctgg aaaagacgga aaaccccttg ccgctcacca
     2341 gtatgtttgg ggatttaaga aaattgcccc agcatccacc aaccagtaat gcaaagtata
     2401 tcatttcgga ttcaggactg aggggaaatg caattttttc aacaagaccg tgggcttcgc
     2461 gccacggcta ttacacaatg tgaccctgcg ggcctaaaac ccttatcctg gcgcttatgg
     2521 ggcttggagc tgactgaaga agcgggctga agaactggtt ttatttcatc cctcatcctt
     2581 catccctcat cccttcaatt gccctcagcc ccacatagtt tgatctatcg tttcccgtcc
     2641 agattggccc attctcctga ttgaatgaaa gctgcccaat agaacggatg ctgccgtctg
     2701 ggatctttga gcatttccaa ctgcacattg cgcaaggctt ctgatcggcc ttcacccgcc
     2761 ttcaaccgcg tgtagtagtc caccatcaat ttccgggttg cctggtctga caccgaccac
     2821 aggctcatca gttgcgcttc gctcccggcc agaaccagtg cccggcgcaa cccatacacg
     2881 ccatcgccgt ttttggcctc gccaatcccg gtttcacagg ctgaaagcgt gaccagtttg
     2941 gtaccccaca aatccagttg g
//
LOCUS       DAOQDO010000573         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26906_length_2958_cov_2.216672, whole genome shotgun sequence.
ACCESSION   DAOQDO010000573 DAOQDO010000000
VERSION     DAOQDO010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26906_length_2958_cov_2.216672"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="cysS"
                     /locus_tag="PKZ53_10170"
     CDS             complement(<1..896)
                     /gene="cysS"
                     /locus_tag="PKZ53_10170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNJ40844.1"
                     /translation="MLKFFNTLTSQLEAFTPLEDNIVRMYSCGPTVYDFAHIGNFRSF
                     LFTDVLHRYLKYRGYQVTFVMNITDIDDKIIERANQTGQNIRTFTDEYLQYFLEDMKQ
                     LDMQFPHHIVRATDHISEMVELVHSLIHNGHAYESEGSVYFRINSFDGYGKLSGAKLE
                     GNKAGARISTDEYEKDDVRDFVLWKGVKPNEPSWPADFGEGRPGWHLECSAMSMKYLG
                     ESFDIHCGGVDLVFPHHENEIAQSEGASGKPFVKYWLHAEHLQINGGKMAKRLGNFFT
                     VRDLLEKGYHPRAIRLALISVP"
     gene            complement(1089..>2958)
                     /locus_tag="PKZ53_10175"
     CDS             complement(1089..>2958)
                     /locus_tag="PKZ53_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40845.1"
                     /translation="AETNAYLQSLVYHVPDGVVTFDERGIIESFNPSAEQILGFSASE
                     AIGKSIQYILGLPSQTSFVPSSTRQETFARRKDGSVFNLEVSVSEFDFRENKRFTAIL
                     RDITSRKQAEIELRNSQQKFEALVNSIDGIVFEVDIETFRFQFVSRQVELLLGYPQQR
                     WIDEPGFWESLLHPDDREEAVRYCKSETTQGRDHEFEYRMIAADGRELWFRDLVRVVC
                     EDGKPRLLRGVMVDITKQRQTEVALHRSQKMEAVGRLAGGVAHDFNNLLTVIKGYSQL
                     LLKSQPPDSIPRQYVEEIKKSADRSSALIRQLLAFSRRQVLQPHVIDLNALILDIREM
                     LERLISEDIELGLSLDPALGRVKADPGQMEQVIMNLVVNARDAMPGGGILSVITGNVE
                     LDEAVVHGRLDAHSGSYVMLATSDTGVGMDEYTKSHIFEPFFTTKAERGTGLGLSTVY
                     GIVTQSNGFIEVESTAGEGTAFSIFLPRVDDDISLIPVSYSEPAHIEGTETILIVEDE
                     EQLRKLVCEALRRCRYQVLEANNGEMALRIYKEHASQIDLLVTDVVMPRLSGPDLAQQ
                     VLAENPAMKVIFMSGYSKNIPLDSGLPLKSIAFLWKPFEPYDLIQKVREVLDSRRR"
BASE COUNT          757 a          772 c          686 g          743 t
ORIGIN      
        1 tagggcaccg aaatcaatgc cagccgaatc gcccgtgggt gatagccttt ttcgagcagg
       61 tcccgcacgg taaagaaatt cccaagccgc tttgccatct tgccaccatt gatctgcagg
      121 tgttcggcgt gcagccagta tttgacaaat ggtttgccgc tcgcaccttc ggattgggca
      181 atttcgtttt cgtggtgtgg aaaaacaagg tccaccccgc cgcaatggat gtcaaatgat
      241 tcgccaagat atttcatgga catcgccgag cattcgagat gccagccagg gcggccttca
      301 ccaaaatcag ccggccagct tggttcgttg ggtttgacgc ctttccagag cacaaaatca
      361 cggacgtcat ctttttcata ttcgtcggtg ctgatgcggg caccggcttt gttgccttcg
      421 agcttggcgc ctgaaagctt gccataacca tcaaaggaat taattcggaa gtagaccgag
      481 ccttcgcttt cataagcatg cccgttatgt atcagtgaat gaaccagttc gaccatctcc
      541 gaaatatgat ccgttgcgcg gacaatatgg tgcggaaact gcatatcgag ctgtttcatg
      601 tcctccagaa aatactgcag gtattcatcg gtaaaggtcc gaatgttctg cccggtctgg
      661 ttggcacgct caatgatttt gtcgtcaata tccgtgatat tcatcacaaa cgtcacctga
      721 tacccacgat atttcaggta ccggtggagc acatcggtaa acaaaaacga gcgaaaattg
      781 ccgatgtggg caaagtcata caccgtcggg ccgcagctat acatgcgaac gatgttgtct
      841 tcgagggggg taaatgcttc gagttgactg gttaaagtat tgaaaaattt cagcatacgt
      901 ttgttttttt gccattgtca gtcgtgagtc ccagggctgg caactcaccg gtttttgggg
      961 aacctgtctg atcaccgctt tttacaattc tgtccacggc gcaacccagg tcagttcagg
     1021 tgactgaatc cctaccgaca acaaccaaaa aacaactggc aattatggag aatgggccac
     1081 ttcaagtttt agcgcctcct tgaatcgaga acttcacgaa ctttttgaat taagtcatac
     1141 ggttcaaaag gtttccataa aaaggcaata cttttcaagg ggagaccact gtcaagcggg
     1201 atgttttttg aataccccga cataaagatc accttcatgg ctgggttttc agccagtacc
     1261 tgctgggcca gatcggggcc actcaacctg ggcatcacaa catccgtcac cagaagatca
     1321 atctgactgg catgctcttt gtaaatccgg agtgccattt ccccattgtt ggcttccaaa
     1381 acctgatatc ggcaccgacg cagggcttca cagaccagtt ttcggagctg ttcctcatct
     1441 tccacgatca agattgtttc ggtaccttca atatgtgctg gttcagaata actgaccggg
     1501 atcaaactga tatcgtcatc cactcggggc agaaagatac taaacgccgt gccttcgcca
     1561 gcagtgctct caacctcaat aaatccatta ctttgggtaa cgattccata cacggttgaa
     1621 aggccaagac ccgttcctcg ttcggctttg gtggtaaaaa acggctcaaa gatgtgggat
     1681 ttggtgtact catccatccc caccccggta tcagaagttg ccagcatgac atatgacccg
     1741 gaatgggcat caaggcgtcc gtgaaccacg gcttcatcca gttcaacatt gccagtgatc
     1801 accgagagaa ttcccccacc aggcatggca tcacgcgcat tgaccaccag attcatgatg
     1861 acctgttcca tctgccccgg atcagctttg actcgtccca gcgccgggtc aagcgagagt
     1921 ccaagttcga tatcctcact gatcaggcgt tccagcattt cgcggatgtc caaaatcaac
     1981 gcattcaaat caatcacatg aggttgcagc acctgacgcc ggctaaacgc caggagctgg
     2041 cggatcaagg cacttgaccg atcagcactt ttcttgatct cttcgacata ctgacgtgga
     2101 attgagtctg gaggctgtga ctttaaaagc aactggctat agcccttaat cactgtcagc
     2161 aggttgttaa aatcatgggc cacgccacct gccagtcttc caaccgcctc cattttctgt
     2221 gatcggtgca gggcaacttc tgtttggcgt tgcttggtga tatcaaccat aaccccgcgc
     2281 agcaacctgg gctttccatc ttcacagacc acgcggacca ggtcgcgaaa ccacagttca
     2341 cgcccatctg cggcaatcat tcgatattca aactcatggt cacgcccctg agtcgtttcg
     2401 gatttacagt accgaacggc ttcttcgcgg tcatctgggt gcaaaaggga ttcccagaaa
     2461 cccggttcgt ctatccaccg ctgttgcgga tacccaagta agagttcaac ttgccggctc
     2521 acaaactgaa aacgaaaggt ttcgatatca acttcaaaga caatgccatc aatcgagttg
     2581 accagtgcct caaatttctg ttgagagttg cgtaactcaa tttcggcctg cttgcgcgaa
     2641 gtaatatccc gtaaaatagc ggtaaagcgt ttattttcac gaaaatcaaa ttcactgact
     2701 gagacttcaa ggttaaaaac cgagccatct ttacggcggg caaatgtctc ctggcgggtt
     2761 gatgacggca caaaactggt ctgactgggt aaccctaaaa tatactgaat tgatttcccg
     2821 atagcctcgc tggccgaaaa cccaaggatt tgttcggctg aaggattaaa cgattcaata
     2881 attcctcgct cgtcaaaagt gacaacgcca tctggaacgt ggtagacaag tgattgtaaa
     2941 taggcattgg tttcagcc
//
LOCUS       DAOQDO010000574         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26955_length_2955_cov_2.700345, whole genome shotgun sequence.
ACCESSION   DAOQDO010000574 DAOQDO010000000
VERSION     DAOQDO010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26955_length_2955_cov_2.700345"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..682)
                     /locus_tag="PKZ53_10180"
     CDS             complement(89..682)
                     /locus_tag="PKZ53_10180"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNJ40846.1"
                     /translation="MKPEITDRVTHLLASRSKQIRADWDYIPAAVLVPIFSKDNIPHL
                     LLTKRSTKLRDHSGEVAFPGGRVDPEDQTPQVTALREAEEEIGLNRQDVRLLGEMDEF
                     LTMSGYLITPVVGWIPYPYDFQINAHEIEYLIEVPIPVIDQPEALEVRQFQVLGRTRP
                     VYFYHYGDRDPIWGVSGRIVNQFLSLVYPILREAVAL"
     gene            775..1449
                     /locus_tag="PKZ53_10185"
     CDS             775..1449
                     /locus_tag="PKZ53_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40847.1"
                     /translation="MTLLAHCFLFSTGRQELRTCSLYFGLTLALMLTGTATTLAQERP
                     AHEIATRISPQNTRPASRPTPPSTQPSEAASPANESPELAIAKELRALVKAVESMRDQ
                     QQAQVAVGFLQVQQARLGALETQIASARAQVAQLKRQQGQNQARSGNIRNELLGRTIL
                     DQAEGERIIRAEIQAEETRLQQEIFEAEQQLTLMQQEYDLKKLETDQIRDQIWNYLRA
                     TPTSNE"
     gene            1826..>2955
                     /locus_tag="PKZ53_10190"
     CDS             1826..>2955
                     /locus_tag="PKZ53_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ40848.1"
                     /translation="MYSLIIRSPGYPPQKVRLVRPQNTIGRSVRADIAIPDSFASRLH
                     ARLRAEENGYILEDLQSANGTYYNGMRVSVPIRLKPGDIIRIGETDLEFASDTIHSVS
                     NLSRTAVFLADSMPATAPEATIGMAGFRATDEILGRASGPLGGKTSVVAPKMPTSASA
                     IEVSGPPTDPKRRLLALVSKVGVALLSNSDLDEVLDQVMNIVFESLPVERGYLMMSNE
                     NQSEPMAAGSLICKVARVGGKAISGEVTLSRHITEQVLVARTAVLTLNAQQDPRFAGQ
                     ESIVLSGTRSVMAVPLALGDQVHGLIYVENPYEQRFTPDDLEVLTTIASVAVIKIENA
                     RLLEERLEKQRLEEEVALAGRIQASMLPRCDPELPGFELAGLS"
BASE COUNT          731 a          816 c          748 g          660 t
ORIGIN      
        1 tatcccttgc cctgctctgc gtcttcagcc cgcgtgattg gtccactttc cgcacaccca
       61 ccttcatcaa accttccatc aaaagatctt acagcgccac ggcttcgcga agaatagggt
      121 acaccagcga caaaaactgg ttcacaattc gccctgaaac accccaaatc ggatctctgt
      181 caccatagtg gtaaaaataa acgggtcgcg tccgacccag cacctgaaac tggcgaactt
      241 ccagcgcttc aggctgatca atgaccggaa ttggcacttc gatcagatat tcaatttcgt
      301 gggcattgat ttggaagtca tacgggtacg gaatccaccc aaccaccggc gtaatcaagt
      361 acccgctcat ggtcagaaat tcatccattt cccccagcaa tcgcacatcc tgacgattga
      421 gtccaatttc ctcttcagcc tcgcgaagcg cagtaacctg tggggtttgg tcttccgggt
      481 caacccgacc gccaggaaac gccacttcgc cactatggtc gcgcagtttt gtggagcgct
      541 tggtcagcaa tagatgtggg atattgtctt ttgaaaaaat tggcacaagc acggctgccg
      601 gaatatagtc ccaatcagca cgaatctgtt tggaacgcga tgccagcagg tgcgtgacac
      661 gatccgtgat ttccggtttc atggcatttc cttcacaagg gttcggtttc cggataccaa
      721 tgtccacagc gaaaccattt caattcaaga atttccgacc aaaaaggatt ccttatgaca
      781 cttttggccc actgctttct tttttctact ggacggcaag aactcaggac ctgctcactg
      841 tatttcggct tgacactggc gttgatgctt accggcacgg ccacgacctt agcacaggaa
      901 cgaccagcac acgagattgc cacgcgaatc tcgcctcaaa atacacgccc agcctcacgt
      961 ccaactcccc catcaaccca acccagcgaa gcggcgagtc cggccaatga atcacccgaa
     1021 ctggcgatag ccaaagaact tcgtgcgctg gtcaaagccg tggaatccat gcgcgaccag
     1081 caacaggccc aggtcgccgt cggctttttg caggtccagc aggcacgtct gggggcattg
     1141 gaaactcaaa ttgcatcggc tcgcgcgcag gttgcgcaac tcaagcggca gcaaggacag
     1201 aaccaggccc gatcaggtaa tatccgaaat gagctgcttg gccggacaat tctggatcag
     1261 gctgaaggag aacggattat ccgcgctgaa atccaggctg aagaaacccg gctccaacag
     1321 gagattttcg aggctgaaca gcaactgacc ttgatgcagc aggagtatga cctcaagaaa
     1381 ctcgaaactg atcaaattcg cgatcaaatt tggaattatc tgcgggcaac tcctacatcg
     1441 aatgaatgaa gattgatgac atttgacact cagccagact acaatcaggg cttctacaca
     1501 atctgcctga ccttcgttcg catcattcaa aaagagagtc acccgtcaat ctggccaggt
     1561 caatattctt tgactgccag cacaacgtct gtctctgtaa aatgtcaggt agcagatttt
     1621 taaatcagtc cacaccggat tggaacactc aggcttgagt tgacggttca gaccatcggg
     1681 ttgagcctgg ctgaatcgct ggccagttcg acggcttcag atcaaaccct gaaccctgaa
     1741 ccctgaaccc gatgtgttcg aatctcctgt ggtttgattg agacattctt catttctaaa
     1801 gacctggagt tgaatcctcg ccgccatgta ctcactgatc attcgttcgc cgggttatcc
     1861 gcctcaaaaa gttcgtctgg tccgtccaca aaatactatt ggacgttcgg tccgggctga
     1921 tattgcgatt ccagattcgt ttgcctcccg tcttcatgcc cgacttcggg cagaagaaaa
     1981 cgggtatatt ctggaagatt tgcaaagcgc caatggcacc tactacaacg gaatgcgggt
     2041 gagcgtgccg attcggctca agccggggga tatcattcgg attggtgaga cggatctcga
     2101 atttgccagt gataccattc actcggtcag caatctgtcg aggacagccg tgtttctggc
     2161 cgatagcatg ccggccaccg caccagaagc gacgattggc atggccggat ttcgggcaac
     2221 ggatgaaatt ctggggcgtg cctcgggacc attgggcgga aaaacttcgg tggtggcacc
     2281 aaagatgccc acgagtgcga gtgcgattga ggtcagcgga ccaccaactg atcccaaacg
     2341 ccggcttctg gcgctggtca gcaaggtggg ggtggcgctc ttatcaaact ctgatttgga
     2401 tgaagtactc gatcaggtga tgaatatcgt ctttgaatcc ctgccggttg agcggggcta
     2461 cctgatgatg agtaatgaga atcagtcaga accaatggcc gcaggctcgc tgatttgcaa
     2521 agtggcccgc gtgggaggga aagccatcag cggcgaagtc accctgagtc gtcacatcac
     2581 cgaacaggta ctggtggccc ggacagcagt tctcacatta aatgcccagc aggatccacg
     2641 ctttgccggt caggaatcca ttgtcctttc tggaacgcgg tccgttatgg ccgtgccgct
     2701 ggcacttggc gaccaggtcc atgggctcat ttatgttgaa aacccatatg agcaacgctt
     2761 tacgcccgat gatctggagg tgttaaccac tatcgccagc gtggcggtaa tcaaaattga
     2821 aaatgcgcga ctgcttgaag aacggctgga aaaacaacgc ctcgaagaag aagtggccct
     2881 ggccggacga atccaggcgt caatgctgcc ccgttgcgac ccggaactgc ccggctttga
     2941 actggccgga ctctc
//
LOCUS       DAOQDO010000575         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_26996_length_2953_cov_2.474810, whole genome shotgun sequence.
ACCESSION   DAOQDO010000575 DAOQDO010000000
VERSION     DAOQDO010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26996_length_2953_cov_2.474810"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2904
                     /locus_tag="PKZ53_10195"
     CDS             <1..2904
                     /locus_tag="PKZ53_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNJ40849.1"
                     /translation="ATDLTVFLGSKPNIFIPTELIPPRPAIRLEHPVLNLQQIAELKA
                     LTTDHSLEVGIRAVVLDTTFDVTEGVAGFDLAIDELTRKGLTAVEHGTAVVILSDRQA
                     GNHRLPIPSLLALRALVNALNESGLKLKASLVVESGEIRSSHHVAALIGFGATAVCPY
                     LALEIARFESSAKLEGIAPEQREANLITALRSGLLKVMAKMGISVLRSYQNARLFTIL
                     GLGRNLVNFYFPGMESVVGGLELEHIVDLVRLNATRSEAAMAVGSGKLLHTYQFREHT
                     QGTAGEKHAMTTTRARMIHKLVRETGHSLSSPELYDQYLKQGEDSAPVTFRHLFRLKT
                     ELPTLGLDQVEPMSDILWRFGTGGMSFGAISAESQRDLITAMREVRARSNSGEGGENP
                     FYFAEGITATTKQVASGRFGVTAEYLISAQEIQIKIAQGAKPGEGGQLMAVKVPPEIA
                     RARHSPVNIDLISPPPMHDIYSIEDLKELIYELKQLHPSAKVSVKLVSGVNIDTIAVG
                     VAKAGADIIHISGGDGGTGAAGLSSMKHAGLPWELGLVAVHRALAANHLRQSITLRVD
                     GGLHTGKDLVLAAALGAEEFDFGKLLLVAEGCIMARICETNRCPAGIATHDPKFKAKY
                     QGTPVHVVRMLEYLAEDVRRHLARLGFATLTDLVGRIDLVETDPRFTQLVTERNLDLS
                     FFFQTVPSGSKPAFDLTTSLAFVPVSPLNEFLVNRAQPALDHQTPLRIECGIRNTDRA
                     ALATLSGVMAETMYRQRGHSEPGTQPPVTSRIQVDFVGSAGQGFGVFLVDGIETRLFG
                     EANDSVCKGMSGGKAVIRPHPHSKLDPEKNTIIGNCALYGATGGKLFINGLAGDRFAV
                     RNSGALAVVEGVGLHACEYMTNGLVVILGKIGLNLGAGMTGGKIYLYGQPDLSRINQN
                     YVQIQQLTNQDTDELTWLLGEYAAATDSLRVQAMVRSFAAHRSEFWKITPGKA"
BASE COUNT          667 a          787 c          842 g          657 t
ORIGIN      
        1 gccaccgatt tgacggtgtt tctgggttcg aaacccaata tttttattcc aaccgaattg
       61 attccgccac gcccggccat ccgacttgaa catccggttc tgaaccttca gcagattgca
      121 gaattaaaag ccctgaccac cgatcattca cttgaagtcg gcattcgggc cgtggtgctc
      181 gataccacct ttgatgtgac cgagggcgtg gccggatttg atttggcgat tgatgaactc
      241 acccgtaaag gattaaccgc ggttgagcat ggcacagcgg tggtgatcct gagtgatcgc
      301 caggcgggga atcatcgcct gccgattcct tccctgctgg ccttgagggc gctggtcaat
      361 gcactcaacg aatcgggatt aaagctcaaa gcgtcgctgg tggttgaatc aggggaaatt
      421 cgcagttcgc atcacgtcgc ggcactgatc ggatttggtg ccaccgccgt gtgtccgtat
      481 ctggccctcg aaatcgcccg gtttgaatcc agtgccaaac ttgaaggcat cgccccggaa
      541 cagcgcgaag ccaatctgat cacagcactt cggtccgggt tgctgaaggt catggccaag
      601 atgggaattt cggtgttgcg cagctatcaa aatgcccggt tgtttaccat tcttgggctg
      661 ggccggaatc tggtgaattt ttatttccca gggatggaaa gcgtggtggg tgggcttgaa
      721 ctggagcaca ttgtggatct ggtgcgactc aacgccaccc ggtcagaagc ggccatggcg
      781 gtcggaagcg ggaaactgct ccatacctat cagttccgcg aacacactca gggtacggca
      841 ggtgaaaaac acgccatgac cacaactcgg gcgcgaatga tccacaaact ggttcgtgaa
      901 accgggcatt cgttgagttc gccagaactc tatgatcaat acttgaaaca gggtgaagac
      961 agcgctccgg tgacgtttcg tcatctgttc cgcttaaaaa ccgaattgcc cacgcttgga
     1021 cttgatcagg ttgagccgat gtcagatatt ctgtggcgat ttggaacggg cggaatgtcg
     1081 tttggtgcca tcagcgccga atcacagcgc gatttgatca ccgccatgcg cgaggtccgc
     1141 gcccgcagca acagcggcga aggtggcgaa aatccctttt actttgctga aggcatcacc
     1201 gccaccacca aacaggtggc ttccgggcgg tttggcgtga cggctgaata tttgatttca
     1261 gcccaggaaa tccagatcaa aattgcccag ggggccaaac ctggcgaagg cggccagtta
     1321 atggcggtaa aagtcccccc tgaaattgcc agggcacgcc attcgccagt caatattgat
     1381 ctgatttccc caccgccgat gcacgacatc tacagcatcg aagacctgaa agaactcatt
     1441 tacgaactca aacagcttca tccgtcggcc aaagtcagcg tcaaactggt gtccggagtc
     1501 aatattgaca ccattgccgt gggtgtggcg aaagctggcg ccgacatcat ccacatctct
     1561 ggcggagacg gtgggacggg tgcggcaggg ctcagttcaa tgaaacacgc cggactgccc
     1621 tgggaattgg gtctggtcgc cgtgcatcgg gcactggccg ccaatcattt gcgccagtca
     1681 attacgcttc gggtagatgg cggcctgcat accggcaaag atctcgtgct ggcagcggcc
     1741 cttggtgcgg aggagtttga ctttggcaag ctcctgctcg tcgccgaagg gtgcattatg
     1801 gcgcggattt gcgaaaccaa tcgctgcccg gcaggcattg ccacccacga cccgaaattc
     1861 aaagccaaat atcagggaac acctgttcac gtggtgcgga tgctcgaata tctcgccgaa
     1921 gacgtccgtc gtcatctggc ccggcttggg tttgcaacgc tcaccgatct ggtgggccgg
     1981 attgatctgg tcgagactga tccacgcttt acccaactcg tcactgaacg caacctggat
     2041 ctgagctttt tctttcaaac cgtgccttcg ggttcgaaac ccgcgtttga cctgaccacc
     2101 agcctcgcgt ttgtcccagt cagcccgttg aatgaatttc tggtcaaccg cgctcaacca
     2161 gcgcttgatc atcaaacgcc gctccggatc gaatgtggca ttcgcaatac tgaccgggcc
     2221 gcactggcaa ccctgtccgg ggtcatggct gaaaccatgt atcgtcaacg gggtcactca
     2281 gaaccaggaa ctcaaccacc agtcaccagc cggattcagg tggattttgt tgggagtgcc
     2341 ggtcaaggat ttggggtatt tctggtggat ggcatcgaaa cccggctctt tggtgaagcc
     2401 aacgattcgg tctgcaaagg aatgtccggc ggaaaagcgg tgattcgccc ccatccacat
     2461 tccaaattag accctgagaa aaataccatc atcggcaact gtgcgctcta tggtgccact
     2521 ggtgggaagt tgtttatcaa tgggctggct ggtgaccggt ttgctgttcg caacagcgga
     2581 gcgctggccg tggtcgaagg cgtcggcctg cacgcctgtg aatatatgac caacggcctg
     2641 gtggtgattt taggcaaaat cgggttgaat cttggcgcgg gaatgactgg cgggaaaatc
     2701 tatctttacg gccagcccga tctttctcgg attaaccaga actatgttca aattcaacag
     2761 ttaaccaatc aggatacaga tgaactgacc tggctcctcg gcgaatatgc ggcggcaact
     2821 gacagcctcc gggttcaagc catggtgcga tcatttgccg cccatcggtc agagttttgg
     2881 aaaatcactc ctggaaaagc ataggggata gggagtgggg gatagggaat gggggacagg
     2941 ggatagggga cag
//
LOCUS       DAOQDO010000576         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27017_length_2951_cov_2.797652, whole genome shotgun sequence.
ACCESSION   DAOQDO010000576 DAOQDO010000000
VERSION     DAOQDO010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27017_length_2951_cov_2.797652"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKZ53_10200"
     CDS             <1..284
                     /locus_tag="PKZ53_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNJ40850.1"
                     /translation="QTAHLLLRLSADALRIGLALAAPVIIVSLMVEFMLAIAGRTIPQ
                     LQILLSGFPIKILICLWLILLTLNFIPGSFRSVAGRTRSAIARVLATSQ"
     gene            322..1383
                     /locus_tag="PKZ53_10205"
     CDS             322..1383
                     /locus_tag="PKZ53_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNJ40851.1"
                     /translation="MSERTEKPTPKRREDARRKGQIARSPKLASAVGFLVAFGIFRVT
                     AQDWLHRVEYLLVSLVPALKATTPLTLQSVHQLFIQAILVIALPVLIVTVAIAIASLA
                     AYVFQGGIVLSTHGLESGPERLNPVSNLKKIFSPQTAIESLKSILELIALIWVCYGVV
                     IQGIVEAPTFIGAPVMVTLATLSALLNSLGLRVGGVLLISSALDYGYKWYSLEKSLRM
                     SKQEIKQEFRDQEGDPMVKGQRRRAARAMLQRRNLAQVATADVVITNPTHFAIAIRYD
                     QNQFAAPIVVAKGVDFVAMRIRELALESKIEIVENPPLARALYVAVEPGQMIPPEFFR
                     AVAEVLAYIYRKNGKQPEL"
     gene            1728..>2951
                     /locus_tag="PKZ53_10210"
     CDS             1728..>2951
                     /locus_tag="PKZ53_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40852.1"
                     /translation="MSFSLINNLSSTGSQQRLSSTTSKLNQTLQRLSSGLRINKSGDD
                     AAGLAIANSYRNDVTTITQGVRNANDGVSTLQIIDGGLNTISGLLDRATTLAAQSASD
                     TFTGNRDTLNSELTKVFDEITRQAENIGLGGASGTNEGRYNKNIGVYIGGGTDASAAN
                     NQVNLDLTNSRVDKTGLNLNNLNIGTGTGSVTGNQSLASGLATAETLTFQKVGSSGNL
                     ESFSIDLSAGASASNVVDTINNDANIQAAGIKAEIDTNNKLVLKSANLFTVSSSDADG
                     ANQTGIAGAGAAANDIAITSAVHSVTKSVGAATAAGSQTLSFSGDEIGVDTGSHSVSF
                     TTSTTAATGASNAATAINSDTTLRSSGVFAVVNKANGSEVSFISLKSFDLAVSNDATA
                     GANNNISSQSPTTAGA"
BASE COUNT          728 a          794 c          730 g          699 t
ORIGIN      
        1 cgcagacggc tcacctgctg ttgcggcttt cggctgatgc cctccgcatc ggactggcgc
       61 tggcggcacc ggtgattatt gtctcgttga tggtggaatt tatgctcgcc atcgctggcc
      121 gcactatccc ccagcttcaa atattgttga gtggctttcc gattaaaatt ctgatttgtc
      181 tgtggttgat cctgctcacg ctcaatttta tccctggctc gttccgaagt gtggctggtc
      241 gaacccgaag cgccattgcc cgtgtgctcg ccacttcaca atgagcgtca gtgaacagga
      301 agacgatttg gtggacctat catgagcgaa cgcaccgaga aaccaacccc gaaacggcga
      361 gaggacgcgc gccgcaaagg acaaattgcg cgtagtccca aactggcaag cgccgttggg
      421 tttctggtgg cattcgggat ttttcgggtc accgcccagg actggctaca ccgggtcgaa
      481 tacctgctcg tgtcactggt tccagctctg aaagcaacca caccgctcac cttacagagc
      541 gtccatcaac tgtttattca ggctatcctg gtgattgcct tgccggtgtt gatcgttacc
      601 gtggcgattg cgattgccag tctggccgcc tatgtttttc aaggaggaat cgttctgagc
      661 acccatggcc tggaatcagg cccggaacga ctcaacccgg tatctaattt aaaaaaaatc
      721 ttcagtcccc aaacggcaat tgaatcactc aagagcatcc tcgaactcat cgccctgatc
      781 tgggtttgtt acggtgtggt cattcaggga attgttgagg caccgacctt tatcggggca
      841 ccggtgatgg tcaccctggc cactttgagt gcgctgctaa actcgcttgg cctgagagtg
      901 ggaggcgtcc tgcttatctc gtcggcactg gattatggct acaagtggta ttcacttgaa
      961 aagtcgctgc gcatgagcaa gcaggaaatc aaacaggaat ttcgcgatca ggaaggcgac
     1021 cccatggtca aaggccagcg ccgtcgggcc gcccgtgcga tgctccagcg tcgaaacctg
     1081 gcccaggtgg ccaccgccga tgtcgttatt accaatccaa cccattttgc aattgcgatc
     1141 cgatatgacc agaaccagtt tgcagcaccg attgtggtgg cgaagggcgt ggattttgtc
     1201 gctatgcgga ttcgtgaact ggccctggaa tcaaagattg aaattgtcga aaatccgcca
     1261 ctggcacgcg cgctctatgt ggctgtcgag cccggccaga tgattccacc tgaattcttc
     1321 cgcgcggtgg ctgaagtcct ggcctatatt tatcgaaaaa atggaaaaca acctgagttg
     1381 taatcaatcc ggaactcaga acactcccag aatttccaaa tacactcaaa tgatttaaat
     1441 caggcagata aaaagcaggg acaggtcagg tttggtcctt gaattgcgtt agaaaaagta
     1501 gctttttact tctccttaat acaaaggctc tctcagtcgg tctcggtcag gccgactttt
     1561 tctttgtctg gaagcctgtt tttccaaata ttccggataa agttctcaag taccctaaag
     1621 tttttgagcc tgagttcgat aagtaaaagt gtagagagca aagtgatcca caaacatcac
     1681 agcgttctta ccaattttga ataacaaaca aaggtggagg gaagaatatg agtttctcat
     1741 taatcaacaa cttgagttca acagggtcac agcaacggct ctcttcaaca acttcaaaat
     1801 taaaccagac acttcaacga ttgtcctcag ggttgcgcat caacaaatca ggcgacgatg
     1861 ccgccggttt ggccattgcc aattcgtacc gcaacgacgt tacaaccatc acccagggtg
     1921 ttcgcaatgc caatgacgga gtctcgacac tccagattat tgacggtggg ttaaacacca
     1981 tttcaggctt gctcgaccgg gccacgactc tggccgcaca atcagcctcc gataccttta
     2041 ctggaaaccg ggacacgctc aactctgaat taaccaaagt gtttgatgaa attacgcgcc
     2101 aggctgaaaa cattggtctt ggtggcgcct ctggaacaaa cgaaggccga tacaataaaa
     2161 acattggtgt gtatatcggt ggcggaactg atgcgtcagc cgctaataat caggtgaatc
     2221 ttgatttgac caattcccgg gttgataaaa ccggactcaa cctcaacaac ctcaatatcg
     2281 gaacgggtac gggcagcgtg actggcaacc agagtctggc ttcaggcctg gccaccgccg
     2341 aaaccttgac cttccagaaa gtgggttcga gcggcaacct ggaatcattt tccattgatc
     2401 tctcagccgg tgcctcggct tcaaacgtgg ttgatacgat taataatgac gccaacatcc
     2461 aggccgccgg gatcaaggcc gaaattgaca ccaacaacaa gttggttttg aaatcagcca
     2521 atttgtttac cgtttcgtca agcgacgctg atggtgccaa ccaaaccggc attgccgggg
     2581 caggcgccgc cgccaatgat atcgccatca ccagtgccgt tcacagcgtc accaagtcag
     2641 ttggggctgc cacagcagcc ggctcacaaa ccctcagttt cagcggtgat gaaattggtg
     2701 tggatactgg gtcccatagt gtgtcattca ccaccagcac cactgccgcc actggcgctt
     2761 ccaatgccgc cacggctatc aacagcgaca cgaccctgcg aagttccggt gtgtttgccg
     2821 tcgtcaacaa agccaatgga tctgaagttt ccttcatctc attaaagagt tttgacctgg
     2881 ctgtttccaa cgacgccacg gcgggtgcca acaacaatat ttcgtcccaa tcaccaacca
     2941 cggcgggtgc c
//
LOCUS       DAOQDO010000577         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27055_length_2949_cov_3.454734, whole genome shotgun sequence.
ACCESSION   DAOQDO010000577 DAOQDO010000000
VERSION     DAOQDO010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27055_length_2949_cov_3.454734"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..2783
                     /locus_tag="PKZ53_10215"
     CDS             219..2783
                     /locus_tag="PKZ53_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ40853.1"
                     /translation="MSTFGVPAQTEKQPDRIDQPIEIQNYKFQKGDDRRWAAPDFDDS
                     GWSSITITKASTAERLTDWQGYVWFRTEVTIPPAMIGQNIAFSYQKNYAHQTADIFLN
                     GVKVGSCGQFPPAPGFENPTTIVIPIPPQVVNTQGKVSLAFRMWAPEDPIFGTARLQF
                     PVKVWWTSAMETQVLAWKRGAMMRHIVQFFLSLMFTLVGIYHYVLYRRRQELTEYFWF
                     SLAALGFAGNTFITSFWAHFWVNSPRLEMRLMTLSQTLTILMGLQFILVFFQYPTSRL
                     IRWYQVALIGVSGLVIILPDRWFHANILFLFYFSVIGLILIGGIIYQKVMQRYVDSIP
                     LGIGIVFVVMSEVLEVVKQSNFRSFLPDIDNYAQYGFASFIFMMAFSLSNRFSRVYSE
                     LDALNQDLEQKVLERTEQITRQRDEITSQKEELEHRKTEVEAKNQELDEKVAELIAAQ
                     KQANRIFSALAEALPGTILDGKYQLAEKIGTGGFGAVFRATQLNLNRLVAVKVFRPAA
                     GNDSEDAIERFRREGVSASRVNHPNAISIVDSGVSSDGIAYLVMELLQGHTLTDELRS
                     TSRLSLSRTLKILIPVCDALSYAHSVGIIHRDIKPDNIFINQSPEGEVVKVLDFGIAK
                     LIGAESGVIAGEQLTGTGLIVGTPAYMPPERLSGKPYDGRADVYSVGIIMFQMITGQL
                     PFAEAQNSLWELILAHTTREIPSLRAYNKNIPEELETLVYSALAKAPESRPSARELAH
                     QLSIVLGNLDDSQVHQVTAPRELRRISTFETTALATGHQAEPRPTSFEGMSDAITVLA
                     ENPGGAATVVAENPGGTATIETQGRANQTQELASLQPHHVANMETIVTERPAPTEE"
BASE COUNT          731 a          730 c          722 g          766 t
ORIGIN      
        1 gtgctatttt ttttacgtga cataggcttt tctaaaatgc ttgaatcaag atgaaggtcg
       61 ttgtgaaggg cacatcctaa agagagtacc gttcagaagc aattccagac tgctgatcaa
      121 tgattcaagg ttgaggaaac aaaatgatga ggttacagca cgtgctcaaa ccattccgtt
      181 cctcgttcat ttgcacaggc tggttgttcc tcgtgtggat gagtacgttt ggcgtacctg
      241 cccaaactga aaaacagcca gaccgaattg accagccgat tgaaatccaa aactacaaat
      301 ttcaaaaagg ggatgatcgc cgatgggcag cccctgactt tgatgattct ggctggtctt
      361 caatcaccat caccaaagcg tcgacggctg aacggttaac tgactggcaa ggatatgtct
      421 ggtttcggac cgaggtgacc attccaccag ccatgattgg tcagaatatt gccttctcgt
      481 atcaaaaaaa ttatgcccat cagacggcgg acattttttt aaatggggtc aaggttggaa
      541 gttgcggtca gtttccaccc gcacctggtt ttgaaaaccc aaccaccatt gtcatcccga
      601 ttcctcccca ggtagtcaac actcagggaa aagtgtcact ggcttttcga atgtgggccc
      661 cggaggatcc gatttttggg accgcacggc tccagttccc agtcaaagta tggtggacca
      721 gtgccatgga aacccaggtt ttagcctgga aacggggcgc gatgatgcgt cacatcgttc
      781 aatttttcct ttccctgatg tttacgctgg ttggtatcta tcattatgtt ctgtaccgcc
      841 ggcggcagga actgactgaa tatttctggt tctcactggc ggcccttgga tttgccggga
      901 acacgtttat tacttccttt tgggctcact tttgggtcaa ttcccctcgt ttggaaatgc
      961 gattgatgac cttaagccaa acgctcacga ttttgatggg gttacaattc atactggtct
     1021 tctttcagta tcccacatcc cggttgattc gctggtatca ggtggctttg atcggagtct
     1081 caggtttggt gatcatcctt ccagataggt ggtttcatgc caatatactc tttctatttt
     1141 atttttcagt gattggcctg atcctgatcg gaggcatcat ttatcaaaag gtgatgcagc
     1201 gatatgttga ttcgattccg ttgggaatcg gaattgtctt tgtggtgatg agtgaagtac
     1261 tggaagttgt aaagcaatca aattttcggt ctttcctccc tgacattgac aactatgccc
     1321 agtatggctt tgcgagtttc atctttatga tggcgttttc actttcaaat cggttcagcc
     1381 gggtttattc agaactcgat gcgctaaatc aagatctgga gcaaaaggtt cttgagcgaa
     1441 ccgaacaaat cacccgtcag cgcgatgaaa tcacaagcca aaaggaagaa ctggagcaca
     1501 gaaagaccga ggttgaagca aagaaccagg aactggatga aaaagtagct gaactcatcg
     1561 cggcccaaaa acaggcgaat cgaatctttt cagctctggc tgaggcactt cctggcacca
     1621 ttctggacgg gaaatatcag cttgcggaaa agattggaac cgggggcttt ggggctgtct
     1681 tccgcgcaac ccagctcaac ctcaaccgcc tggtggccgt caaagtgttt cggccagccg
     1741 caggaaatga ttctgaggat gccattgagc gatttcgccg cgaaggcgtc tcagcctcac
     1801 gtgtcaacca tccaaatgcg atttcgattg ttgattccgg agtttcaagc gacggaattg
     1861 cctatctcgt catggaatta ctccaggggc ataccttaac cgacgaactt cggtccacct
     1921 cacggctttc gttgtcccga acgctgaaaa tcctgattcc ggtgtgcgat gcgctcagtt
     1981 atgcccattc cgtggggatc atccatcggg atatcaaacc tgacaatatc tttatcaatc
     2041 aatcacctga aggagaagtg gtcaaggtgc ttgattttgg gattgccaag ttgatcgggg
     2101 ccgaatcggg ggtcattgcg ggcgagcaac tgaccggcac cggtttgatt gtcggtaccc
     2161 cggcctatat gccgcctgag cgattgagtg gaaaaccata tgatggacgc gctgatgtct
     2221 acagcgttgg tatcatcatg ttccaaatga tcaccgggca actccccttt gccgaagcgc
     2281 aaaacagtct ctgggagtta atcctggccc ataccacacg tgagatccca tcccttcgag
     2341 cctacaacaa aaacatcccg gaagaactgg aaacgctggt gtattcagct ttggcaaaag
     2401 ctcctgaatc ccgaccatca gcccgcgaac tggcgcatca actcagtatc gtgctgggga
     2461 atttggacga tagccaggtg catcaggtca cagcaccccg ggaactgcgg cgtatttcga
     2521 cctttgaaac gactgccttg gccacggggc atcaagctga gcctcggcca accagtttcg
     2581 aaggcatgtc ggatgcaatt accgtcctgg ccgaaaatcc aggcggtgct gccaccgtcg
     2641 tggccgaaaa cccaggcggt acggccacca ttgaaaccca ggggagggca aaccagactc
     2701 aggagcttgc cagcctacag ccccaccacg tcgccaatat ggaaacaatc gtcactgaac
     2761 gaccagctcc aactgaagaa taacgtatag tattgaatac caatgtatta gaccattctt
     2821 cattcttcat tcttcattct tcatttcaaa aatttgtact cgcattgagg gcgatttccg
     2881 tgtataacca ggatggtttg agggtgccct acgaagattg tttcctttca tcctcgtcat
     2941 ggatcttct
//
LOCUS       DAOQDO010000578         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27059_length_2949_cov_2.882170, whole genome shotgun sequence.
ACCESSION   DAOQDO010000578 DAOQDO010000000
VERSION     DAOQDO010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27059_length_2949_cov_2.882170"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            546..1382
                     /locus_tag="PKZ53_10220"
     CDS             546..1382
                     /locus_tag="PKZ53_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HNJ40854.1"
                     /translation="MKPSLLVAKSDPSRQTQIPLGSSVVGSSELVVIAGPCTIESYTQ
                     ARLVADTLVANGISCMRGGIFKPRTSPYSFQGLHEEGFEIIQAIRRETGLAIVSEVMS
                     IEQLDLAVDQFDCIQIGSRNMQNFELLKALGKINRPVLLKRGLAATIEEFLLAAEYVL
                     ASGNPNVILCERGIRSFDPATRNVLDLASVALLKEQTHLPVIVDPSHATGKRSLILPA
                     SRAAVAVGADGLIVEIHPVPEQSVSDADQAISLEEFTRLLTQIEPIASAVGRTMFECV
                     AG"
     gene            1443..2897
                     /locus_tag="PKZ53_10225"
     CDS             1443..2897
                     /locus_tag="PKZ53_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNJ40855.1"
                     /translation="MSKQNSALQSPTELPFMVTVLSDVETPASLFHKLCSDEPTAFLF
                     ESAEGDTRLARYSLIGMDPVLTVTFHNGTATVIRTETKTTTEFCFDNPLNVLERLLAQ
                     EQQRFTATLPADFPFAGGFVGYLGYAATQYFDRIPQPETRPFGIPEGFYGLYDTFIVF
                     DHLYRKIKFISYRSQSHLNHLLEKLSSTPHSLRPLIAPQPPTTEPDLFEGVQTVFDRE
                     RFCNVVKKCKEFISAGEVFQIVPSQRFFVPIESQPFDIYRNLISLNPSPYAYFLKFPG
                     FTYLGSSPETFVECRQGKVVLRALAGTRPRGTTPEADEALAQELQSNEKELAEHFMLI
                     DLERNDLGRVCEVGSVAVGEIAQIIRYSHVMHLATEVSGTLRADKTVFDVFQSCFPRG
                     TVSGAPKIRAMQLLAQLEPEQRGIYSGVVGYIDFNGNADGAIAIRSATVKDGIAHLNA
                     GAGVVYDSDPEAEYEETRNKAKSVLRAIVSTKIQ"
BASE COUNT          719 a          771 c          730 g          729 t
ORIGIN      
        1 cacacaccgt tgacttgtca cctcaaaaat tggtttacac cagtaacttt ttcatcaggc
       61 gctcaatttt cacattattt tgtccctggc ctgtctgaaa ctgcggggcg gaactttttt
      121 cagggcgtta ccagatatat ttgagatacc ccattgtgag gatataagcc gctgatgttg
      181 aatggaaaga ggcagttaat atcttcactt ttccaggttg cggagggttg aagtcccact
      241 cagtctcagg aaaatactgg ttgacaaatc cgtgaaagtt tccttatatc ttggccagat
      301 tttcaacccg ttttcataca ttttcaggac tcaaatgaac acgcaattct ttacggcaca
      361 atattggtgg tattactggt ttagcccgcc ggaggcctgc ccaattgtgt caacctgaag
      421 cgttgccgtg tcgagatcga aacaccaacg aaattcaaaa gaccgcaggc ccccaaactc
      481 cggggcctgc ggtttttgct ttttccgaac actttttcag cccatagaaa gcgagatcaa
      541 acacgatgaa accctccctt ctcgtcgcaa agtccgaccc gagccggcaa acccagatac
      601 cgctgggaag ttcggtcgtc gggagttcag aactggtggt gattgccggc ccctgcacca
      661 tcgagtctta cacccaggcc cgcctcgttg ctgacaccct cgttgccaac ggcatttcct
      721 gtatgcgcgg cggcattttc aaaccccgaa cttcccccta tagcttccag ggactccacg
      781 aagaaggctt cgaaatcatc caggccatcc gccgcgaaac tggactggcc atcgtcagcg
      841 aagtgatgag cattgaacag cttgacctgg ccgtggacca gtttgactgt atccagattg
      901 gttcgcgcaa tatgcagaac ttcgaactgc ttaaagcact ggggaaaatc aatcgcccgg
      961 tgctgctcaa acgcggactg gccgccacga ttgaagaatt cctgctggcc gctgaatacg
     1021 ttctcgcctc tggcaatcca aacgtgattt tgtgcgaacg gggcatccgc agttttgatc
     1081 ctgccacccg caatgtgctt gatctggcct cagtggcgct gctgaaagaa caaacccacc
     1141 tgcccgtgat tgttgatcca agccacgcca ccggaaaacg gagtttgatt ctgccggctt
     1201 cgcgggcggc tgtggcggtt ggggctgatg ggttgatcgt cgaaatccat ccggttccag
     1261 agcaatcagt ttcggacgct gatcaagcca tttcactgga agagttcacc cgactcctga
     1321 cccaaatcga accaattgcc agtgcggttg gccgaacgat gtttgaatgt gtcgctggct
     1381 aaaaaatgag cgaacagtca gtaccacctg cgtcagcggg tgggtaaaag cgagaaatgt
     1441 gcatgtcaaa gcaaaattca gccctacaat cacctactga actcccattt atggtcacgg
     1501 ttttgtcaga cgttgaaaca ccagcgtcgc tctttcacaa actgtgttct gacgaaccga
     1561 cggccttttt atttgaaagt gctgaaggcg atacccgact ggcacggtat tcattgattg
     1621 ggatggatcc ggtgttgacg gttaccttcc acaacggaac ggcaaccgtc attcgaactg
     1681 aaacaaaaac cacaactgaa ttttgctttg acaatccttt gaacgttttg gaaaggctgc
     1741 tggctcagga gcagcaacga tttacggcaa cgctaccggc tgactttccg tttgccgggg
     1801 gattcgtcgg atatttgggc tatgcggcaa cgcaatattt cgaccgaatt ccacagccgg
     1861 aaacgagacc gtttggaatt ccggaagggt tttatgggct gtatgacacc ttcatcgtgt
     1921 ttgatcacct ctaccggaaa atcaagttta tctcgtaccg aagccaatca caccttaatc
     1981 acctgcttga aaaactctca tccactcctc actcgctccg tccgctgatt gctccccaac
     2041 ctccgacaac tgaacctgat ctttttgaag gcgtccaaac tgtgtttgat cgcgagcggt
     2101 tttgtaacgt cgtcaaaaaa tgcaaagaat ttattagtgc cggtgaagtg tttcagattg
     2161 tgcccagcca gcggtttttt gtcccaattg aaagccagcc atttgatatc taccgcaatt
     2221 tgatcagctt aaacccttcg ccgtacgcct attttctgaa attccctggg tttacctatc
     2281 ttggttcatc gccagaaacc tttgttgagt gccgccaggg aaaagtcgtg ttgcgggcgc
     2341 tggccggaac gcgtccacgc ggcacaaccc cggaagctga tgaagccctg gcccaggaac
     2401 tccaatcaaa tgaaaaagaa ctggccgagc atttcatgtt gattgacctg gaacgcaacg
     2461 acctgggacg ggtctgtgag gtcggaagcg tcgccgtcgg cgagattgcg caaatcatcc
     2521 gctatagcca cgtcatgcat ctggccacgg aggtcagcgg cacgcttcgc gctgacaaaa
     2581 cggtgtttga tgtcttccag agttgttttc cacgcggtac ggtttctggc gcacccaaaa
     2641 tcagagccat gcagcttctg gctcagcttg aacccgaaca gcgcgggatt tactcaggcg
     2701 tggttgggta cattgatttt aatggaaatg cggacggtgc gattgcgatt cggtcagcga
     2761 cagtcaaaga cggtattgcg catctgaatg caggtgcagg cgtggtctat gattctgacc
     2821 cggaagccga atatgaagaa acccggaata aagccaaaag tgtccttcgg gcaattgtgt
     2881 caaccaaaat ccagtagggt tcagggttct tcgaagggat gaaggatgaa agaaatcatc
     2941 ttttttcac
//
LOCUS       DAOQDO010000579         2946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27110_length_2946_cov_3.044967, whole genome shotgun sequence.
ACCESSION   DAOQDO010000579 DAOQDO010000000
VERSION     DAOQDO010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27110_length_2946_cov_3.044967"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..280
                     /locus_tag="PKZ53_10230"
     CDS             113..280
                     /locus_tag="PKZ53_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40856.1"
                     /translation="MVKKIGVYVWIACLTCAAGQPVIELTHHIAKPGHIAKPGHIVRP
                     GTGSSVLGMQA"
     gene            330..1694
                     /locus_tag="PKZ53_10235"
     CDS             330..1694
                     /locus_tag="PKZ53_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40857.1"
                     /translation="MRKYVASLLLLFSLLTVYMPLPAVTADQSGTTANVEVLMPGGLL
                     ERTNTVAYTANGRYGVIASAQARPGDTDPNSGQYAYAFDASTGAITDQYWLGPTPSRT
                     VLHKLTSQVAIVHQSKEPETFGVGCISLIAIDGAGQFNQSPHLNSLLPEPIQIFLPAC
                     GNPDQYAPEVLDEIWMHPQTPVAIVSNKAALFAVSLIDGTILNRIEMIPPDLFEDGWR
                     ISDFDVNVQTGRIAVLKRSNDGRFSLSFVRYTHPGSFTFLGETLRVVDIVGDQSNVAL
                     NEDGTEAFVAASGSGSLYRFTLQSIRISTFRLPFMQAESSEEAEVTPLIVSSLANSTR
                     LAIQKPGHIQKPGHIQKPGHITLINDTKGTINFVAEQTFSSPLTNLSFDRSGTLGFVG
                     TLNGEVFQFSSVDGQFTQMPMGLQDAISLTYDDTHQRLSIGTNLAQTPTGTAVSAVRL
                     VDLQ"
     gene            1829..2704
                     /locus_tag="PKZ53_10240"
     CDS             1829..2704
                     /locus_tag="PKZ53_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ40858.1"
                     /translation="MLLCRLIFLITLVLIACVQVGAGVLQRHTLTGDIRVHPQFHSSF
                     LPQDRDILVYLPPNYEKHTTKRYPVLYMHDGQNLFDGATSFIPGAEWRVDETAQTLIT
                     AGQIRPVIIVAIYNTRDRIAEYTPTPDPEYPSGGKADLYARMLVEEVKPFIDRTYRTK
                     PDACNTGLAGSSLGGLVSLHISLKYPGTFGRVAVISPSVWWNNRGIVNEVSSLSAKVP
                     LRIWLDIGTQEGTSSIPNTRLLRDALTTKGWMLNKDLRYFEAEGAGHNEGAWALRVEP
                     ILKFLFGPGSTKLRC"
BASE COUNT          715 a          709 c          762 g          760 t
ORIGIN      
        1 aggttgtaaa cgataagcct ctttttgctt atcgttgatg ggtgccgacc acgaccaggc
       61 ttttttgttt ggtcaagctc aaactgttga aaaataaaga tttgaggatt gtatggtaaa
      121 aaaaatcggt gtttacgtct ggattgcctg cctgacctgt gcggctggcc agccggtgat
      181 tgaactcact caccatatcg ccaagccggg acatattgcc aaaccaggtc acatcgtgcg
      241 accaggtact ggatcgtcag tcttggggat gcaagcctag tgcatctggc gggcgggtcc
      301 cgcgagcaag agtgggcatt ggggggaaca tgcgaaaata tgtagcgtcg cttttattgc
      361 tattcagttt gctgacagtc tatatgcctt tgccggcagt aaccgccgac caatcgggga
      421 ctacggccaa tgtggaagtc ttgatgccag gtgggttatt ggaacgaacc aataccgtgg
      481 cctataccgc taatggtcgg tatggcgtga ttgcgtcagc tcaggcccga cctggggata
      541 cagatccaaa ttctggtcaa tacgcctatg cgtttgatgc gtcaactggt gccattaccg
      601 atcagtactg gttggggcca acaccaagcc gaacagtttt gcataaactg acgtcacagg
      661 tggcgattgt tcatcagagc aaagagccag aaacgtttgg ggtcgggtgc atcagcctga
      721 ttgcaattga tggcgccggc caatttaatc aatcaccaca tctcaactca cttttaccag
      781 agccgatcca aattttcctt ccagcttgtg gaaatccaga ccagtatgca cccgaagtcc
      841 tggatgaaat ctggatgcac ccgcaaacgc cagttgcgat tgtttcaaat aaggccgcct
      901 tgtttgccgt cagtttaatt gatggcacga ttttgaaccg aatcgaaatg atacctcccg
      961 acctttttga agacgggtgg cgaatttctg attttgatgt caacgttcaa actggccgca
     1021 ttgccgtctt gaagcgatca aacgatggac ggttcagcct gagctttgtg cgctacactc
     1081 atcccggcag ctttaccttt ttgggtgaaa cgttgcgggt ggtggatatt gtgggcgacc
     1141 agagcaatgt ggctttgaat gaagacggca ccgaagcttt tgtggctgcc agcgggtctg
     1201 ggtcactgta tcggtttact ttgcaaagta tccggatttc aaccttccgc ctgccattta
     1261 tgcaggctga aagcagtgaa gaagctgaag tcaccccgtt gatcgtctcc tcactggcca
     1321 acagtacccg cctggcgatt cagaagccgg gtcatatcca gaaaccaggt catatccaga
     1381 aaccagggca cattacgttg atcaacgata cgaagggcac gatcaacttt gtggcagagc
     1441 agacgttttc gtcaccgttg accaatttat cttttgatcg gagtggcacc cttgggtttg
     1501 tggggacgct caacggcgag gttttccaat tttcgtctgt tgacgggcaa ttcacccaaa
     1561 tgccaatggg gctgcaagat gccatcagtc tgacctatga cgacacccat caacggttaa
     1621 gcattggtac caatctggca cagaccccaa ctggaactgc ggtcagtgcc gttcgtctgg
     1681 ttgacctcca ataaattgaa tcaatttttt tgagttttgg accccgtttc ttctgaccgc
     1741 aatggttggc aggaaatggg tttcaaaact caaattcatt ttgtagaggc tcaggtttca
     1801 gcactccaaa aatagataag attcatctat gttgctctgc cgattgattt tcttgattac
     1861 tctggttttg atagcctgtg ttcaggtggg tgctggagtc ctccagcggc acacactgac
     1921 gggggatatt cgggtccatc ctcaatttca ctccagtttt ttacctcaag accgggatat
     1981 tttggtgtat ttgccaccaa attatgaaaa acacactaca aaacggtacc cggttctcta
     2041 catgcacgat ggccaaaacc tctttgacgg agcaacgtcg tttatccccg gtgctgaatg
     2101 gcgcgttgac gaaacggctc aaactttaat cactgccggc cagatccggc cagtcattat
     2161 tgtggctatc tacaacactc gggatcgaat tgctgaatac acgccgactc ccgatccgga
     2221 ataccccagc ggtggcaaag cggatttgta tgcgcggatg ctggtggaag aagtgaaacc
     2281 cttcattgac cggacctatc ggactaaacc tgacgcctgt aacaccgggc tggcaggttc
     2341 ctcgctcggt ggtctggttt cattgcatat cagcttgaaa tatccgggta catttgggcg
     2401 ggttgccgtg atttcaccat ctgtttggtg gaataatcgt ggtatcgtca atgaagtttc
     2461 atctctgtct gccaaagtgc ctctgagaat ctggcttgac atcggcaccc aggaaggcac
     2521 cagcagcatt ccaaatacaa ggttgttgcg tgatgctctg accaccaaag gctggatgct
     2581 taacaaagat ttgcggtatt ttgaagcaga gggtgccggg cacaacgaag gtgcctgggc
     2641 gttacgagtc gaacccattt taaaatttct gtttggacca ggaagcacca aattaaggtg
     2701 ttaggcagga ctggggttga gggctaaagg ggaacccctg gttgtcttca ctaccaaacc
     2761 ttgagcccga aaccctgagt cccaaatccg gaggggacta tggctatcaa accaactgat
     2821 gcaaaaaaac tcgccgatca gtttaatacc cgtgaactct cgcatctgat tgaagtctca
     2881 gatgaattga tgcgcgtggt gatggaagcc ggtgagtttg acaaagctga aggtgccgcc
     2941 caggcg
//
LOCUS       DAOQDO010000580         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27143_length_2944_cov_4.348910, whole genome shotgun sequence.
ACCESSION   DAOQDO010000580 DAOQDO010000000
VERSION     DAOQDO010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27143_length_2944_cov_4.348910"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1406
                     /locus_tag="PKZ53_10245"
     CDS             36..1406
                     /locus_tag="PKZ53_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNJ40859.1"
                     /translation="MSSFKSLSITGWGRYPVVTSEVYRPERVREVSAILADLHDKALI
                     ARGYGRSYGDASLNAGKRVVVSERLNRMLAFDDQTGLLKCEAGVQLKDILDVFVPRGW
                     FLPVTPGTKFPSLGGSFACDVHGKNHHRDGSISRHTHEIELMLASGDIVRCTPTENSE
                     LFWATAGGMGLTGILLTVTIRMQRIESAYISVDFRKTRNLAETMDLCESEDDRYHYAV
                     CWIDCLASGKSIGRSVLMHGEHARRDQVPAKFAKDPLRLHKKTALTIPMDFPGWAINP
                     LSVKLFNFGYYHKHPQNKMGVITDYDTYFYPLDFVLEWNRIYGRRGFLQYQCVFPLET
                     SRQGMFKVLEILSQQQIASFLAVLKRFGPQEGLLSFPMPGYTLALDIPISNQRMFDTL
                     NRLDELVVAEGGRLYLGKDARMSAAHLARMYPKLDEWKRIKHQVDPEGIFSSDLSRRV
                     GLCGVR"
     gene            1476..2234
                     /locus_tag="PKZ53_10250"
     CDS             1476..2234
                     /locus_tag="PKZ53_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ40860.1"
                     /translation="MTPQLSQKRYLILGASSAMARAVAEELARRGNALDLAGRDLEDL
                     ERTAADLAIRYEVQARAISFDALDLKSHTSFAQKILTEPGSTLEGVILLFGTMTDQKE
                     AEQDFRQAELMIAGNYTGAASILGYFANAFEVRKSGVIVGVSSVAGDRGRQSNYIYGS
                     AKGALSLYLQGLRNRLFHSGVRVITVKPGFVDTAMTYGLKLPPIVAPPSRVACDICRA
                     MDGQADVVYTPFFWRYIMLIIQHIPEFVFKRLKL"
     gene            complement(2259..2705)
                     /locus_tag="PKZ53_10255"
     CDS             complement(2259..2705)
                     /locus_tag="PKZ53_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ40861.1"
                     /translation="MDTFEERIIIRALPDQIMPYLIEPELIELWSPTEVKVTLLSRRK
                     TGPGTKFQFEFLEHGLDPITYVVVTQTPTLLVCSFEGRMVGEDRWELTKQRGNSTELY
                     NRMEFRAPDTLTWIGWKAAGRAIAFQTAREKMPLIKEVIEEACAKS"
BASE COUNT          696 a          696 c          796 g          756 t
ORIGIN      
        1 ttcttaattc ttaattctta attcttaatt cttaaatgtc ttccttcaaa tcgctttcga
       61 tcaccggttg gggacgctac ccggtggtaa cttctgaagt ctatcggcca gagcgagttc
      121 gcgaagtctc agctattctg gccgatctgc acgacaaagc cctgattgca cggggctatg
      181 gccggagcta tggtgatgct tcgctcaatg ctggaaaacg tgtggtggtg agtgagcgcc
      241 tcaaccggat gcttgccttt gacgatcaaa ccggattgct caaatgtgaa gccggggtgc
      301 aattaaaaga catcctggat gtgtttgtgc cgcgtggctg gtttttacct gtcacgccag
      361 gaacaaaatt tcccagtctt ggtggctcgt ttgcctgcga cgtgcatggg aaaaaccatc
      421 accgggacgg ctcgatttcg cgccataccc acgaaatcga actgatgctg gcctcaggtg
      481 acattgtgcg ctgcacgcca actgaaaaca gcgagttgtt ttgggctacg gctgggggaa
      541 tgggactgac gggaatcttg ctgacggtaa ctatccgaat gcagcgcatt gaatcagcct
      601 acatcagcgt tgattttcgc aagactcgga atctggcaga aacaatggac ctctgcgaat
      661 ccgaagacga ccggtatcac tatgcggtgt gctggattga ttgtctggct tcgggcaaat
      721 ccattggacg cagtgtcctg atgcacggcg aacacgctcg acgtgaccag gtgccagcaa
      781 aatttgccaa agatccgctc cgattgcaca aaaaaacggc gttgaccatc ccgatggact
      841 ttcccggctg ggcaatcaat ccgctttcag tcaagttgtt caatttcggc tattaccaca
      901 aacatccgca gaataagatg ggtgtcatta ctgactacga tacctatttt tacccgctgg
      961 attttgtgct ggaatggaat cggatttatg gccggcgagg ttttttgcag tatcagtgtg
     1021 tctttccact tgaaaccagc cgccagggaa tgtttaaggt gcttgaaatt ttgagccagc
     1081 aacaaattgc ttcgtttctg gcggtgctca aacgctttgg cccccaggag ggcctgcttt
     1141 catttcctat gccgggctac acgctggcgc ttgatattcc aatcagcaat cagcgcatgt
     1201 ttgacaccct gaaccggctc gatgaactgg tggtggcgga aggtggccgt ctttacctgg
     1261 gcaaagatgc ccggatgtct gccgcccatc tggcgagaat gtacccaaaa cttgacgagt
     1321 ggaagcgcat caagcatcag gtagacccgg aaggcatttt ttcatctgat ctttcccgcc
     1381 gggttggctt atgtggagtg cggtgacttg acaccgcttt gtattttatg tggagtgcgg
     1441 cggcttgacg ccgctttcca gcaaggacgg acactttgac tccccagtta tctcaaaaaa
     1501 gataccttat tttgggcgct tcatcggcga tggcacgggc tgtcgctgag gaactggcgc
     1561 gccgtggcaa tgcacttgat ctggctgggc gtgatctgga ggacctggag cgaaccgccg
     1621 ctgatcttgc gatccggtat gaggttcagg caagagctat ttcatttgat gcgctggatc
     1681 tcaaatccca tacctctttt gctcagaaga ttctgactga acctggctcg acattggaag
     1741 gggtcatcct gctctttggc acgatgaccg atcaaaaaga agctgagcag gattttcgac
     1801 aggcggaatt gatgattgcg ggaaattata ccggggcagc ttcgattctc gggtattttg
     1861 ccaatgcctt tgaagtgcgc aaatcagggg tgattgtcgg ggtgtcatcg gtggccggag
     1921 atcgtggccg gcagagtaat tatatctatg gatcagccaa aggggcgttg agcctgtatt
     1981 tgcaggggtt gcgcaaccgg ctctttcaca gcggcgtgcg ggtgattaca gtcaaacctg
     2041 gatttgttga taccgctatg acgtatgggt taaagctccc gccaattgtg gcgccgccga
     2101 gccgggtagc ttgtgacatt tgccgggcga tggatggcca ggcggatgtg gtctacacac
     2161 catttttttg gcgctacatc atgctcatca tccagcacat tccggaattt gtgtttaagc
     2221 gccttaaatt gtgataggga ttgatacaac tggcaaaatc aggattttgc gcaggcttct
     2281 tcaatcactt ctttgatcaa aggcattttc tcccgagctg tttggaaagc aatggcccga
     2341 ccagcggctt tccagccaat ccaggtcagg gtatcaggtg cccgaaactc catccggtta
     2401 tagagttctg tcgaatttcc cctctgtttg gtcaactccc accggtcctc ccccaccatt
     2461 cggccttcaa acgaacacac cagcaatgtc ggggtctgag taaccaccac ataagtaatt
     2521 ggatccaggc catgttcaag gaattcaaat tggaattttg ttccgggccc ggtcttccgc
     2581 ctggaaagca gcgtgacttt gacttcggtt ggtgaccaga gttcaatcaa ttccggttcg
     2641 atcaggtagg gcatgatctg gtccggcagg gcgcgaatga tgatgcgttc ttcaaaagta
     2701 tccatgatgt tgagggggaa ggggaaaaga atgaagaatt gagaatgaag aatgaagaat
     2761 tgagaatgaa gaatgaagaa agtggttcgt ggttagtttc tggtgcttag tacttcgtgg
     2821 ttgaaaatca ataccttcga agaagaatca agaaccacag agtggtgagt ggtgagaaat
     2881 caatgctttc caagaaccaa gaaccaagaa ccaagcacca aaaaccaaaa actaacccgt
     2941 ttct
//
LOCUS       DAOQDO010000581         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27144_length_2944_cov_3.895466, whole genome shotgun sequence.
ACCESSION   DAOQDO010000581 DAOQDO010000000
VERSION     DAOQDO010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27144_length_2944_cov_3.895466"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKZ53_10260"
     CDS             complement(<1..625)
                     /locus_tag="PKZ53_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNJ40862.1"
                     /translation="MCGRYTLSVEEPVVLDRFGLTTTELILTPRYNIAPTQPIPVVLN
                     TSPETLSAAKWGLIPAWAKDAKMAATMINARSETLLEKPSFRTILKSQRCLVVADGFY
                     EWKKNEDGTKTPYRVQLDSGEVFAMAGLWDTWKAPDGTLLRTCTIITTTANDVLAPLH
                     ERMAVILPPETERLWLEQNTPNTLLDLLQPYPSEKMMAYEVSSRVNSI"
     gene            819..1835
                     /gene="hpnH"
                     /locus_tag="PKZ53_10265"
     CDS             819..1835
                     /gene="hpnH"
                     /locus_tag="PKZ53_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HNJ40863.1"
                     /translation="MGISLRQAVTVGAYVLKQRFKGVKRYPLVLMLEPLFRCNLECAG
                     CGKIQYPEEILKKNLSPEECFRAAEECGAPVVTIAGGEPLIHPKIDEIAKGLVERKKF
                     VYLCTNAILLERHMHKFTPSPYFTFSIHLDGMKKRHDELVCREGVFDVAVRAIKAAKA
                     AGFRVTTNTTVFDGENPEEMHEMFDFLADLGVDGMTISPGYSYAWAPDQEHFLRREQT
                     HALFRQVLSPMRTERGKRWQFNQSSFFLDFLMGERDYECTPWGNPNYNVFGWQKPCYL
                     LNEGYAKTFGELLEKTKWEEYGRKSGNPKCQDCMVHCGYEPTAVIDATANLKNMLISA
                     KSLR"
     gene            complement(1916..2587)
                     /locus_tag="PKZ53_10270"
     CDS             complement(1916..2587)
                     /locus_tag="PKZ53_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40864.1"
                     /translation="MPVLKRALTLMVIAVVGWGIFSVPVVGKSDSPSLPAAPDLVQQV
                     VQSFDALTDYRCQMYSSRLRKDGTMAVDTNNYFFKKPKLIRLEVTSGGDKGSKVVLNK
                     KGKVRAKAGGVLGVFTITMEPTDKRLQDGDSSFVTTDLGSTLKDLQLTMNGAKATVTL
                     IEGPRRAYQLVLERPGKKDTILIDAELKLPLQWEVFRNGKFDSKTEWKNLTKDVGLAD
                     ELFEL"
     gene            2783..>2944
                     /locus_tag="PKZ53_10275"
     CDS             2783..>2944
                     /locus_tag="PKZ53_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HNJ40865.1"
                     /translation="MKTLLLNPPSFEGFDGGASSRWPATREIASYWYPVWLAYPAGMI
                     PNSRLLDAPP"
BASE COUNT          734 a          725 c          724 g          761 t
ORIGIN      
        1 gaattgagtt cactcgcgat gagacttcat aggccatcat cttttctgaa ggatagggtt
       61 gcaacaaatc taacagggtg ttgggcgtat tttgttctaa ccacaggcgt tctgtctcag
      121 gcggtaaaat aacagccatt cgctcatgaa gcggtgccaa cacatcattg gccgttgtgg
      181 taataatggt gcaggttcgc aggagcgttc catctggtgc tttccaggta tcccacaacc
      241 cagccatggc aaaaacttct ccgctgtcga gttgtacccg gtacggggtt ttggtcccat
      301 cttcattttt cttccactca taaaagccgt cagctaccac caggcaccgc tgagatttca
      361 gaatggtgcg aaatgatggt ttttcgagca gcgtttccga ccgggcatta atcatggtgg
      421 ctgccatttt ggcatctttg gcccaggcag ggataagccc ccatttggcg gctgaaagtg
      481 tttccggcga ggtatttaac accacgggaa tcggctgggt tggggcaatg ttgtagcggg
      541 gtgtcaagat caactcggtt gtggtcaatc caaaccgatc caggacaaca ggttcttcaa
      601 ctgacagggt atagcgtcca cacataatga atgaagaata tagaaatgaa gaattaggaa
      661 atcggatcgg ggatatcgtc ctcaaacaat tgaacgattg accacccaac ccggcaatat
      721 gcgtttgtca aacccctcgg atttgatacc ttcttgcccc atcataccca aatttccaag
      781 aaagttcaga ttatcctcac tgttttacct tcatcgttat gggcatttca cttcgacaag
      841 cagtcaccgt tggtgcatac gttttaaagc aacgttttaa aggagtaaag cgctatccgc
      901 tcgtgctgat gcttgaacct ttattccggt gtaatcttga gtgcgccggt tgtggcaaaa
      961 tccagtatcc ggaagaaatt ctgaaaaaaa acctctctcc ggaagaatgt tttcgtgccg
     1021 ctgaagaatg cggcgccccg gttgtcacca tcgcaggtgg cgaaccgctg attcatccca
     1081 aaattgacga aattgccaaa gggctggttg agcggaaaaa atttgtttac ctgtgtacca
     1141 atgccatttt gcttgaacgg catatgcaca aattcacccc gtcaccttat ttcacgttta
     1201 gtatccacct cgatggcatg aaaaaacgcc atgatgagct ggtatgccgc gaaggtgtct
     1261 ttgatgtggc cgtccgggca attaaagctg ccaaagctgc cggcttccgg gtgaccacca
     1321 acaccacagt gtttgacggt gaaaaccctg aagaaatgca cgaaatgttt gatttcctgg
     1381 ctgatctggg ggttgatgga atgaccattt ccccagggta cagctatgcc tgggcacctg
     1441 atcaggaaca ttttctgcga cgtgaacaaa cccatgcctt attccggcag gtcttgtcac
     1501 caatgagaac cgaacggggc aaacgctggc agtttaacca gtcctcgttc ttccttgatt
     1561 tcctgatggg cgaacgtgac tatgaatgca cgccgtgggg caatccaaac tacaatgtct
     1621 ttggctggca aaaaccctgc tacctgctca atgaaggcta tgccaaaacc tttggtgaat
     1681 tgcttgagaa gaccaaatgg gaagaatatg gccggaagag tgggaatccg aaatgtcagg
     1741 attgcatggt tcactgtgga tatgagccaa cagcggtaat tgatgcgacc gccaacctga
     1801 agaacatgct catttcggca aagagtctgc gataaggtca gtaccactcg cctcagcggg
     1861 tgggtaatgt gcaaacaaaa cccactcgct ggcgcaggaa aaactgacaa agtagttata
     1921 attcaaagag ttcatctgcc aggccaacat ctttggtcag atttttccac tctgttttgg
     1981 aatcaaattt cccattgcga aagacctccc actggagcgg gagtttgagt tcggcatcaa
     2041 tgagaatcgt gtctttcttt ccaggccgtt ccaggaccag ttgataggct cgccgaggac
     2101 cttcaatcag ggtgacagtg gctttggcac cattcatcgt cagttgtaaa tctttgaggg
     2161 tagaccccaa atcagtggtg acaaaactgc tgtcgccatc ctggagccgt ttgtcagtgg
     2221 gctccatggt aatggtaaac acccctaaaa ccccaccggc tttggcgcga accttgccct
     2281 ttttattcaa aacgactttt gaacctttgt cgccgccgct ggtgacttcc agacgaatca
     2341 gttttggctt tttgaaaaag taattgttgg tatctactgc catggttcca tctttgcgca
     2401 agcgggatga atacatctgg cagcgataat cggtcagggc atcaaagctt tgcacgacct
     2461 gttgcaccag gtccggagca gcaggaagcg atgggctatc actctttccc acgactggga
     2521 cagagaaaat tccccaaccg acaaccgcaa ttaccatcag tgtcaaagct cgtttcagca
     2581 caggcatgtt gaaaagttcc tttcaattcc agagattgtt cgatattctg gcgctcccct
     2641 gcggggtcaa ttacaccact ggttcctgac ttgacagtca ctgggctagt ttacagggtg
     2701 cgaccccgga cccacaggct ggttttgatc gcgccaggac tcatatttcg tttcatttaa
     2761 agcgaggcag tgcatgaatt tcatgaagac gttgctctta aacccacctt cgtttgaagg
     2821 atttgacggt ggcgccagtt cccggtggcc ggcgacccgc gagattgctt cctactggta
     2881 tccagtgtgg ttggcttatc cggcaggaat gattcccaat tcacggcttt tggatgctcc
     2941 tcca
//
LOCUS       DAOQDO010000582         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27154_length_2944_cov_2.452060, whole genome shotgun sequence.
ACCESSION   DAOQDO010000582 DAOQDO010000000
VERSION     DAOQDO010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27154_length_2944_cov_2.452060"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKZ53_10280"
     CDS             <1..699
                     /locus_tag="PKZ53_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40866.1"
                     /translation="LLCFTVSCKDGARLPVESDQPTSLHNQVERGEVSVDGIVLTTNP
                     EQTIYGRNIDFKITNQTDHPIAVAIETGTVLRSDNQQYYTNLVVSSGEQALIAPKSLA
                     IITVEVFSINFQKFSPVRSTRYSLGNLIEGDSKTFLDCFARRRPETGVAPSALSQSAP
                     APTQTGKVDLAPVQLAIWLMTDNMDRAALRKAVSSNPQLKGGQYSQSVKYYDAMAPFV
                     QKLLDDCGLGKYKF"
     gene            841..1770
                     /gene="fabD"
                     /locus_tag="PKZ53_10285"
     CDS             841..1770
                     /gene="fabD"
                     /locus_tag="PKZ53_10285"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNJ40867.1"
                     /translation="MGSIAFLFPGQASQYAGMGKALAEAFSEARTVFEEADASLGFSI
                     SQLCFEGPEADLALTANTQPAILTVSVAAYRVLASQGLTPAFVAGHSLGEYSALVAAG
                     TLSLTDAVVAVRRRGTYMQEAVPPGVGAMAAVLKADLATIEAICAEAAQDQVCSPANM
                     NSPAQIVIAGHAEAVTRAAALLKAKGARAVMLPVSAPFHCALMKPAEERLAVDLNQLD
                     FADPAFPVVANVSADLERTGAEARANLIAQVCSPVRWVGSIQKLVAEGVTTFVEVGPK
                     TVLSGLVRQINNQVKVLNFEQPDQLEAIREACS"
     gene            1940..2185
                     /locus_tag="PKZ53_10290"
     CDS             1940..2185
                     /locus_tag="PKZ53_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNJ40868.1"
                     /translation="MSAIEEKVRQIIVEELGVDESEVTPKARFIDDLGADSLDTVELV
                     MRFEEEFGIEIPDEDAEKITSVREAVTYIEAHLESLK"
     gene            2207..>2944
                     /locus_tag="PKZ53_10295"
     CDS             2207..>2944
                     /locus_tag="PKZ53_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNJ40869.1"
                     /translation="MKRRVVITGVGIVSPVGNTAEETWAALLAGRSGISQITHFDTTG
                     FSVHIAAEVKNFDPAKFIEKKEIKKMDPFIHYAIAASQEALDDSGLKITDDNSERVGI
                     FVGSALGGFTIIEREQTKLFTGGPDRISPFFIPSSIINLAAGQMSIRFGIKGPNSAVS
                     TACAAGAHSVGDAYRLIRRGECEAMLCGGAEATITPMGIGGFAAMRALSTHNDRGPQA
                     SRPFDRLRDGFVMGEGAGILVLEELEHA"
BASE COUNT          718 a          784 c          771 g          671 t
ORIGIN      
        1 ttgctgtgtt ttaccgtcag ttgtaaagat ggtgcccggc ttccggttga gtccgatcag
       61 ccaaccagtc tccacaacca ggttgaacga ggtgaggttt ccgtagatgg cattgtgtta
      121 accaccaatc ctgaacagac catctatgga cgcaacattg actttaaaat caccaatcaa
      181 actgatcacc cgattgccgt ggccattgaa actggcacgg tactgcgaag cgacaatcag
      241 cagtattaca ccaacctggt ggtgtcatcc ggtgaacagg cactgattgc gccaaaaagt
      301 ctggcaatca ttacggttga ggttttttcg atcaatttcc agaagttttc accagttcgc
      361 tcaaccaggt acagtttggg aaacctgatc gaaggcgatt ccaaaacctt tcttgactgc
      421 tttgcccgac ggcggcctga aactggcgtt gcaccatcag cactttcaca atcggcacca
      481 gccccaactc aaactgggaa agttgacctg gcaccagtcc agcttgccat ctggctgatg
      541 actgacaata tggatcgcgc tgccctacgc aaagcagttt caagcaaccc tcagttgaaa
      601 ggtggacaat acagccagag cgtcaaatat tatgacgcca tggcaccgtt tgttcagaaa
      661 ctgctcgatg actgtgggct gggcaaatac aagttttaag ggaccgagaa gaaaataaaa
      721 tcccaacgaa atcgggttct gggtttgaaa agacgaaagg gacaaaaagg acaaaaattc
      781 gaacccctga accctgaacc ctgaaccctg actaccacct gaaatcaaca atcactcatt
      841 atgggatcaa ttgcattttt atttcctgga caggcttcgc agtacgccgg catgggcaaa
      901 gcactggctg aagccttttc cgaagctcgc actgttttcg aagaagccga cgccagttta
      961 ggattttcca tcagtcagct ttgctttgaa ggccccgagg ctgacctggc gctgacagcc
     1021 aacacccaac ccgcgatttt gaccgtttcg gtcgccgcct atcgggttct ggccagtcag
     1081 ggcctgacac cagcctttgt ggccggtcat tcactcggcg aatacagcgc gctggtggcc
     1141 gctggaacac tgtcattaac agatgcggtg gtggcagtcc ggcggcgtgg cacctatatg
     1201 caggaagcag ttcctcctgg cgttggcgcg atggcagcag tcctcaaagc tgatctggcc
     1261 actatcgaag ccatctgtgc cgaagccgcc caggatcagg tctgcagccc tgcgaatatg
     1321 aattccccgg cccaaatcgt catcgctggc catgccgaag ccgtcacccg tgccgccgcc
     1381 ttgctcaaag ccaaaggtgc ccgcgccgtg atgctccccg tgagcgcccc cttccattgt
     1441 gcgctgatga aaccggctga agaacgactg gcggttgatt taaaccagct tgattttgct
     1501 gacccggcct ttccagtggt ggctaacgtc agtgccgacc tggaacgaac tggtgctgag
     1561 gctcgcgcca atctgattgc tcaggtttgc tcacccgtca gatgggtcgg ctcaattcag
     1621 aaactggtgg ccgaaggtgt gacgactttt gttgaagttg ggccgaaaac agtgctcagc
     1681 ggcctggttc gtcaaatcaa caatcaggtc aaagttctca attttgaaca gccggatcag
     1741 ttggaagcga tccgtgaagc ctgttcctaa acgggtcagc gggattggca acacgcttgc
     1801 aaccaggcac gttcccgctt ccctggcagc tttcgcaagt tgaatctttc gattgtgact
     1861 tgcttctttg aaccaaaaaa aataaggtac gcaaaatttt tttcacaaat caggaggaag
     1921 gacctttttt atgtcagata tctcagctat tgaagagaag gtgcggcaaa tcatcgtcga
     1981 agaattgggg gttgatgaaa gcgaagtaac accgaaagct cgctttattg atgacctggg
     2041 tgccgattca ctcgacacgg tcgaactggt catgcgtttt gaagaagagt tcggaatcga
     2101 aattccggac gaagatgctg aaaaaatcac cagcgtccgt gaagccgtga cctatatcga
     2161 agcccacctc gaatcactca aatagtcctg ttttttggag gcccctgtga aacgtcgcgt
     2221 tgttatcacc ggagtcggca ttgtcagccc cgtggggaac accgctgaag aaacctgggc
     2281 agcgttgttg gcgggtcgaa gtgggattag tcaaattacc cacttcgata ccaccgggtt
     2341 ttcagtccac atcgcagccg aagtcaaaaa cttcgatccg gcaaaattca tcgagaaaaa
     2401 agaaatcaag aaaatggatc ccttcatcca ttacgccatt gcggcatcgc aagaggcatt
     2461 ggatgacagt ggtctcaaaa tcaccgacga caacagcgaa cgagtcggca tttttgtcgg
     2521 ttcggcgctg ggtgggttca ccattatcga acgtgaacag accaaactgt ttaccggtgg
     2581 cccagaccgg atttcgccct tctttattcc atcatccatc atcaacctgg cagcaggcca
     2641 gatgtcgatc cgatttggca tcaaaggtcc aaactcggcg gtttcgacgg cctgtgcggc
     2701 tggggcgcat tcggtcggag atgcctatcg cctcatccgc cggggcgagt gtgaggccat
     2761 gctctgtggc ggcgccgaag ccaccatcac gcccatgggc attggcgggt ttgctgccat
     2821 gcgtgccctg tcaacccata atgaccgggg tccacaagcc agccggccat ttgatcggtt
     2881 gcgggatggg tttgtgatgg gcgagggcgc tggaatcctg gtgcttgaag aactggagca
     2941 cgcc
//
LOCUS       DAOQDO010000583         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27164_length_2943_cov_3.629848, whole genome shotgun sequence.
ACCESSION   DAOQDO010000583 DAOQDO010000000
VERSION     DAOQDO010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27164_length_2943_cov_3.629848"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2943)
                     /locus_tag="PKZ53_10300"
     CDS             complement(<1..>2943)
                     /locus_tag="PKZ53_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ40870.1"
                     /translation="YTSGSTGNPKGVVVTHRNVVRLFTATDDWFHFGPGDVWTLFHSF
                     AFDFSVWELWGALLFGGRLVVVPYLTSRSPEAFYHLLLTEQVTVLNQTPSAFRQLQHE
                     DQHHSADALTLRLVIFGGEALEPGSLRPWVDRHGLEQPQLINMYGITETTVHVTYRPI
                     TLADVERGSVSPIGTAIPDLQLFVLDQHGQPVPVGVPGELYVSGAGVARGYLNRPELT
                     ADRFGFRVPGSGFRVSDQETALSSEIQPDEVLTVDSEDLSRFQAMIPGTRNPEPGTRF
                     FKPGTRNPEPGTRLWYRTGDLVRWLPSGELEYLGRIDQQVKIRGFRIELGEIEAALHQ
                     HPQVRESVVLLREDVPGDRRLVAYVVAQSVEGKPGESLRGELLEYLRLKLPDYMVPAA
                     IVFLETIPLTSHGKVDRKALPEPVATTEALEEIALPQTQTEEILAALWAEVLGLEQVD
                     IHTSFFDLGGHSLLATQVLSRMKETFEVTLPLRVMFENPTVVAIATAIDEARNAQNAS
                     AETVPPLVRISRDNPLPLSFAQERLWLIDQLEPGRVHYSIPAVFRVKGDLNLDALEQA
                     FRALVERHEILRTTYATDNRTPVQIIQSEFPAESWTVEPETDPGLIKQRVVADILKPF
                     DLANGPIFRAKITQLSENEWILVIVIHHIAFDGWSIGVLIRELSALYQAFANRTPLNL
                     PALPIQYADFAHWQRMWLQGEVLESQLAFWKNQLSGAPTVLELPTDFARPPVQSFQGA
                     LRQISLSPELSNQIKLLARNEQTTLFVVLLAVFETLLYRYTGQDDVLVGTPVAGRTRC
                     ELEGLIGFFVNTLVMRARFEPGLTFRQFVAQVRETVVSGLAHQDVPFEKLVEALQPDR
                     DLTRTPVFQVVFVLQNAFNTPNLKRRFGSLEIEPVEAENLNQAGFSKFDLTLIAREQA
                     GSIRLGMQFNTELFRPETIDRMLKHLHQLMESAVTTPDLPLAVLPMLSQTELNQLLVQ
                     WNPTRTFES"
BASE COUNT          729 a          780 c          789 g          645 t
ORIGIN      
        1 ggattcaaag gtccgggttg gattccactg aaccagcaat tgattgagtt cggtctgact
       61 gagcattggc aagacggcca acggaaggtc aggagtcgtt accgcgcttt ccatcaactg
      121 gtgcaggtgt ttgagcatcc ggtcaatggt ttcaggccgg aacagttcgg tattaaactg
      181 catcccaagg cgaatgcttc cagcctgttc gcgagcaatc agcgtgagat caaatttcga
      241 gaagcccgcc tgattcaggt tttcagcttc gaccggttca atttcgagtg acccaaatcg
      301 tcgtttcaaa ttcggcgtgt tgaaggcatt ttgcagcaca aacaccacct ggaagactgg
      361 tgtccgggtc aaatcgcggt ctggctgcag ggcttcgacc agtttttcaa acggaacatc
      421 ctgatgcgcc agcccggaaa cgacggtttc ccgaacctgc gctacaaact gtcgaaaggt
      481 cagcccaggt tcaaatctgg ctcgcatcac cagcgtattg acgaaaaacc caatcaagcc
      541 ttcgagttcg cagcgggtcc gtcccgccac cggagtcccc accagcacat catcctgccc
      601 ggtatagcga tacagcaagg tttcaaacac agccagtaac acgacaaata gcgtcgtttg
      661 ctcgtttcga gccagaagtt ttatctggtt ggaaagttcg ggcgaaagtg aaatctgtcg
      721 aagcgcccct tgaaacgatt gtactggcgg gcgggcaaaa tcagtcggca gttcaaggac
      781 cgtcggagcg cccgaaagct ggtttttcca gaaggccagt tgtgattcca aaacttcgcc
      841 ttgcaaccac atccgttgcc agtgagcgaa atcagcatat tggattggta atgccggcaa
      901 gttcaatggt gttcgattgg caaacgcctg atacagtgca ctcaattcac gaatgagaac
      961 tccgattgac catccatcaa aagcaatatg atgaataaca atcaccagaa tccattcatt
     1021 ttctgaaagt tgagtgattt tcgctcggaa aatggggccg ttcgctaaat caaacggttt
     1081 gaggatgtca gcgacaactc gttgtttgat caatcctggg tctgtctctg gctcaaccgt
     1141 ccagctttcg gctggaaatt cagattgaat gatttggact ggcgttcgat tgtcggtcgc
     1201 gtaggtggtt cgcaaaattt cgtgccgttc aaccagggcg cggaatgcct gttcgagtgc
     1261 gtccaggttt aagtcacctt tcacccgaaa cacggctgga attgagtaat gcacccgacc
     1321 tggttcaagc tggtctatca gccagagccg ttcctgggca aatgacaacg gtaacgggtt
     1381 gtcacgcgaa attcgaacca gcggaggcac cgtttcagca ctcgcatttt gagcattccg
     1441 ggcttcatca attgcggtgg caatagcgac cacagttggg ttttcaaaca tcacccgaag
     1501 cggcagcgtg acttcaaagg tttctttcat tcgcgaaaga acctgcgttg ccagtagcga
     1561 atgcccaccg aggtcaaaga agctggtgtg aatgtcaacc tgttcgagtc caaggacttc
     1621 ggcccaaagt gcggctaaaa tttcttcggt ttgagtctga ggcagggcaa tttcttcaag
     1681 tgcttcagtc gtcgcgaccg gctctggcag tgcctttcga tcaactttgc cgtgagaagt
     1741 cagcggaatg gtttccagaa agacaatcgc cgctggaacc atgtagtccg gcagttttaa
     1801 ccgcaaatat tccagtaact caccgcgaag agactcaccc ggtttacctt caaccgattg
     1861 agccacgacg taagccacca gccggcggtc tccgggaaca tcttcccgca gcagaacaac
     1921 cgattcgcga acctgtggat gttgatggag cgcggcttca atttcaccca gttcaatccg
     1981 gaagccacga atcttgacct gctgatcaat ccggcccagg tattcaagtt cgccagatgg
     2041 cagccagcgg acgagatccc ctgtgcggta ccaaagccgg gttccgggtt ccgggttccg
     2101 ggttccgggt ttaaaaaacc gggttccggg ttccgggttc cgggttccgg gtatcattgc
     2161 ctgaaatctc gacaaatcct ccgaatccac tgtgaggact tcatctggtt gtatctcaga
     2221 agacagagct gtttcttggt cagaaacccg gaacccggaa cccggaaccc ggaacccgaa
     2281 tcggtcggcg gtcaactccg gacggttcaa atagccacgg gcgaccccag cgccggaaac
     2341 atagagttcc ccagggacac caaccgggac gggctgtccg tgctggtcaa gaacaaacag
     2401 ttgcaggtca ggaatcgccg ttccaatcgg actcactgaa ccgcgttcga catcagccag
     2461 cgtgatgggc cgataggtga cgtgaaccgt ggtttcggtg atgccataca tgttgatgag
     2521 ttggggttgc tccaatccat gccggtcaac ccagggacgg agcgagccag gttcgagggc
     2581 ttcgccaccg aaaatgacca gtcgaagggt cagcgcatcg gctgaatgat gctggtcttc
     2641 gtgttgaagc tgacgaaagg ccgaaggggt ctggttcagc accgttacct gttcagtcag
     2701 cagaaggtga tagaaggctt cgggtgaacg tgaagtcagg taagggacaa ccaccagcct
     2761 cccgccaaag agcaacgctc cccagagttc ccagaccgaa aaatcaaaag caaacgaatg
     2821 aaacagcgtc cagacatcac ctggaccaaa gtgaaaccag tcatcagtgg cagtaaacaa
     2881 ccggaccaca ttccgatggg taaccacgac gccctttgga ttgccagtcg aaccggaggt
     2941 gta
//
LOCUS       DAOQDO010000584         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27188_length_2942_cov_2.692761, whole genome shotgun sequence.
ACCESSION   DAOQDO010000584 DAOQDO010000000
VERSION     DAOQDO010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27188_length_2942_cov_2.692761"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..2441)
                     /locus_tag="PKZ53_10305"
     CDS             complement(87..2441)
                     /locus_tag="PKZ53_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNJ40871.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIDAEHILLGLMREDKGL
                     TNRFLVRAQISIESIRRDIESRTIPRERTSPTVDLPLSPEAKRVLTFAAEESERLSHR
                     HIGTEHLLLGLLREENSVAARILYERGLRLNAIRDELSRTSGSDRHATQKKETPHLAE
                     FSRDLTESARQGNLDPLIGRTNEVERVIQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVSGEVPHFLADKQILALDLSLVVAGTKYRGQFEERLKTIMRELVENPQYIIFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PAPSEDEAIRILEGIKEKYEEFHHVTYTQDAIETAVFQSNRYIPDRFLPDKAIDIIDE
                     AGSQVKLRVSHLVNSRTSRRPGPGPNATANLDAELKMWDQSVAMVDKEDIESVISRWT
                     GIPMNSIKEEEMEKLMRLEGELHKRIVSQDQAISALSRAIRRSRAGLKNPNRPVGSFL
                     FLGPTGVGKTEVARSLAEFLFGSERAMIRFDMSEFMEKHSVSKLIGSPPGYVGHEEGG
                     QLTERVKRNPYSVLLFDEIEKAHPDIFNILLQVFEDGQLSDSLGNTIDFKNVIIIMTS
                     NIGARFIQKRGSLGFQPSAGAQQTRIEDAVMSEVRHTFNPEFINRLDEIIIFEPLSED
                     DLEQIVHLLVNQLNHTMVRRHLSLSLASEAAKWIVTRTASERSYGARPLRRAIQRYIE
                     DPLSESVIQGRFETGTHISVLLEDGEIQFRPVPVEEPGGALVTH"
     gene            complement(2718..2879)
                     /locus_tag="PKZ53_10310"
     CDS             complement(2718..2879)
                     /locus_tag="PKZ53_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40872.1"
                     /translation="MFDLVILYSIKSPEIGTHRVQGSGFDPKQSTWPATQQGLTQWSE
                     SEVFQSRVD"
BASE COUNT          715 a          712 c          785 g          730 t
ORIGIN      
        1 agaaccaaga accaagaacc aagaaccaag aaccaagaac caagaaccaa gaacgaacta
       61 ccaggtacga agcaccaggc acgttcttaa tgggttacca gcgcgccacc aggttcttcg
      121 actgggacgg gccggaattg gatttcgcca tcttcgagca aaactgaaat gtgagttcca
      181 gtttcaaacc tgccttgaat cactgattca gacaatgggt cttcgatata gcgctgaatt
      241 gcccgacgta agggacgagc accataggag cgttcggagg cggttcgggt gacaatccat
      301 ttggcggctt cggaggccag tgacaggctc aaatgtcgtc gcaccatggt gtggttcagt
      361 tgattgacca gcaggtgcac aatttgttcc agatcgtctt ccgacagggg ttcaaagatg
      421 atgatttcgt ccaaccggtt gatgaattcg ggattaaagg tgtgccggac ttcgctcatc
      481 acggcatctt caattcgggt ttgctgggca ccggctgagg gctggaagcc caaactgccg
      541 cgtttttgga tgaaacgggc gccgatgttt gaggtcatga tgataatgac gtttttaaag
      601 tcaatcgtgt tgcccaggct gtcagataat tgcccatctt caaaaacctg gagcaggatg
      661 ttgaaaatgt cgggatgggc tttctcaatt tcgtcaaaga gcagcactga ataaggattg
      721 cgtttgacgc gttcggtgag ctgaccgcct tcttcgtggc cgacgtagcc tggaggcgag
      781 ccaatcagct ttgacacgga atgtttttcc ataaattccg acatatcaaa gcgaatcatg
      841 gcgcgttcgc tcccaaacaa aaattcagcc agtgaacggg caacttccgt ttttccaacc
      901 cccgtgggcc ccaggaacag gaagcttcca accgggcgat tcgggttttt taagccggca
      961 cgtgaccgcc gaatggcgcg tgaaagggca gaaatggcct gatcctgact gacaatgcgt
     1021 ttgtgtaatt caccttcgag ccgcatcagt ttttccatct cttcttcttt gatggaattc
     1081 atcgggatac cggtccagcg tgaaatcaca ctctcgatat cttctttatc aaccatggcc
     1141 acactttgat cccacatttt caactcagca tcaaggttgg cggtggcatt tggaccaggg
     1201 cctgggcggc gtgatgtccg cgaattgacc agatgtgaaa cccgaagttt cacctgggat
     1261 ccagcttcgt caatgatatc aatggcttta tcggggagga accggtcagg aatatagcgg
     1321 tttgattgaa agaccgccgt ttcgatagcg tcctgggtgt aggtaacgtg gtgaaactct
     1381 tcgtactttt ctttgatgcc ttccagaatc cgaattgctt catcttcgga gggtgctgga
     1441 actttgacgg cttgaaagcg gcgttcgagt gaacggtctt tctcaataga tttgcgaaat
     1501 tcagcaggtg tggtggcccc aatgcactga atttccccac gagaaagcgc tggttttaag
     1561 atattggcgg catcaagcga cccttctgcc gacccggcac caaccagcgt gtgcaactca
     1621 tcaataaaaa tgatgtactg cgggttttcg accagctccc gcataatcgt tttcagtcgt
     1681 tcttcaaact gtccgcgata tttggtgccg gccacgacca ggcttaaatc cagggccaga
     1741 atttgtttgt cagccagaaa gtgcggtact tcgccgctga caatgcgctg ggccagtcct
     1801 tcaacaatcg ctgttttccc aacccctggt tcgccaatca gaaccgggtt gtttttggtg
     1861 cgacggcaca aaatctgaat cacccgttcg acttcattgg tgcgaccaat gagcgggtcc
     1921 agatttccct ggcgggcaga ttcggtcaaa tcccggctga attcagccag atgaggtgtt
     1981 tctttctttt gggtggcgtg tcggtcagac cctgaagtcc gggacagttc atcacggatg
     2041 gcattgaggc gcaggccacg ctcatacaaa atccgggccg cgacgctgtt ttcttcccgc
     2101 agcaatccca aaagcaagtg ctcagtgcca atatgacgat gggatagccg ttccgattct
     2161 tcagcagcaa aggtcaaaac ccgtttcgcc tcaggggaaa gcggtaaatc aacggtcgga
     2221 gaggtacgtt cgcggggaat ggttcgcgat tcaatatcgc ggcggatgga ttcaatcgaa
     2281 atttgtgcac gcaccaggaa gcggttggtt aagcctttgt cttcgcgcat cagaccgagc
     2341 aggatatgtt cggcatcgat ggcaggcgcc ccgaactgac tcgcctcgta acgagcgaaa
     2401 aagataacgc gacgggcttt ttcagtgtat ctctcaaaca ttctttattc cttattgtct
     2461 gctagggtac caagttgcct ctggtggctc tggaatcgtg gctggcaggg gtctttgaag
     2521 agcggaaatt catcaggcaa cttcagcaag atgaaaaatt ataacgacag gctggcaaaa
     2581 ggcaaaaggg aattatccaa aatctggcag gttgctggtg gtggccagga tggtttgggg
     2641 ttcggggttc agggttcagt ctttaataag actagaagtc tttgtagttc aaagaattat
     2701 agggatttgt acctgaatca atccacacgg gattggaaca cttcagattc cgaccattgg
     2761 gttaagcctt gctgagtcgc cggccaggtt gattgtttcg gatcaaaccc tgaaccctga
     2821 acccgatgtg ttccaatctc cggtgatttg attgagtaca ggatgaccag atcaaacata
     2881 tcccacggat gaagcggtat caggccagcc ggccatgttc aaaatggagt tgtcgagtac
     2941 ac
//
LOCUS       DAOQDO010000585         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27309_length_2935_cov_2.965278, whole genome shotgun sequence.
ACCESSION   DAOQDO010000585 DAOQDO010000000
VERSION     DAOQDO010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27309_length_2935_cov_2.965278"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="PKZ53_10315"
     CDS             complement(<1..1784)
                     /locus_tag="PKZ53_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40873.1"
                     /translation="MPINKISDQTKLEFDDFDLDRAQKLADYIFRGQSQTLSRKIAWQ
                     SVNLTVINVAQGTEGARMFRRLQKRVIKPVKTKSQVLQNMVYAESEIYERYQEGEDLA
                     ELKAKAKTSDPFANLLAELRRLPLSQEDRIIRYFKFLTKWSVELKAVHVFLALEHLAR
                     NTTVKEVAGIYQEVFDPELEIKHISTALNAMAEKVLIRFRRNLLPNGITSIPSKKDCY
                     SFLGSLLSEVSDPTDLQVVSHCRTKFVPWDTRCIRPDILRSIRLVNPQLTEKAVETVQ
                     PILQSSINQSNPGSPAEHEIKLRQLLAWALGQPHLTLEDIRRIQYHLKDEIVQAIERI
                     LKDVQEGDQKEVTFSHVVICPDCADIIQNLYKRRQPVMIPKPFLSQKDTPSAGTSKPP
                     ATQASGDQSHSDSLQDFLDHQAKRRRLARPGPVTVFSDGRQVALLDPARESVARFQID
                     RLDQLVKVTASDSEGILELASLLVCPKEIEDSFEFRWLKRKSYCRQLENAERLVFVIR
                     PDQSTGETDEYCIELKIQAPQMSKNWLGRNLFQPVPRWVWVPIALAIIFGVYGIVQTL
                     FLPNPKPGPVVGLPPKPSPVSPPQSGTP"
     gene            2102..2308
                     /locus_tag="PKZ53_10320"
     CDS             2102..2308
                     /locus_tag="PKZ53_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40874.1"
                     /translation="MKQHEEAQGYELTPRTVTSLVAISTIPTTASSADGVIKGNILSR
                     VEAVRQQQGGSVGESCPWSASWPD"
     gene            2438..>2935
                     /locus_tag="PKZ53_10325"
     CDS             2438..>2935
                     /locus_tag="PKZ53_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40875.1"
                     /translation="MTIPMTLNKGLKLRLPADMPVESVTINSGTYLVPSQGNEPRCIL
                     PKPDWRQLTLEEYDRLVSVQDVPLQESVSVINVDEHVRTIFEDHIARQLFGQPQLAPD
                     QAKAVLEGFAQQAAQSLQIAGWSVRPVVCNATINPPNLPSTAFDFEHGSLVGLHFDNR
                     ETNFHL"
BASE COUNT          735 a          716 c          724 g          760 t
ORIGIN      
        1 tggggcgtac ctgattgagg tggagaaact ggactgggtt tgggtggcag ccccactaca
       61 ggacccggtt ttggattcgg cagaaagagg gtctgcacga tgccatatac cccaaagatt
      121 atagcgagtg cgataggaac ccatacccat ctgggcactg gctgaaaaag attccgtccc
      181 agccaatttt tactcatctg tggagcctga atcttgagtt cgatgcagta ctcatctgtt
      241 tcaccagtag attgatcagg tcgaattaca aaaaccaggc gttctgcgtt ttccaattgc
      301 ctgcaatagc tctttcgctt gagccaacga aactcaaaag agtcttcgat ttctttgggg
      361 caaactaaaa gtgatgccaa ctcaaggatt ccttcagaat cggatgccgt tactttcaca
      421 agctgatcca ggcggtcaat ctgaaacctg gcaactgatt cacgcgccgg gtcaagcaaa
      481 gcaacttgac ggccatcact aaatacggtc acgggccctg gcctggcgag acgacggcgt
      541 ttcgcctggt gatcaaggaa gtcctggagt gagtcagaat gagattggtc acctgaggct
      601 tgtgtggctg gaggcttgct ggtaccagcg gagggcgtat ccttctgact cagaaacggt
      661 ttgggaatca taactggctg cctccgcttg tacaggtttt ggatgatatc cgcacaatcc
      721 ggacagataa cgacgtgtga aaaggtcact tccttttgat ccccttcttg cacatccttc
      781 agaattcgct cgatagcctg cacaatctca tctttgaggt gatattgaat tcttcgaatg
      841 tcctccaatg ttaagtgtgg ctgacccaaa gcccaggcca gcaactgccg gagtttgatc
      901 tcatgttcag ccggtgaacc tgggttggac tgatttatag atgattgcag gattggttgt
      961 accgtttcaa ctgctttttc agtgagttgg ggattgacta atctgattga tctcaagata
     1021 tcaggtctta tgcatctggt gtcccagggc acaaacttcg tgcgacaatg gctgactacc
     1081 tgcaaatcgg tggggtcaga aacttcactc aacaaactcc ccaaaaaact gtagcagtct
     1141 ttttttgaag gaattgatgt gatcccattg ggcagcagat ttcggcgaaa gcgaatgaga
     1201 actttttccg ccatcgcatt cagtgccgtt gagatatgct tgatctcaag ttctggatca
     1261 aacacttcct ggtaaatacc tgcgacctct ttgaccgttg tattccgggc cagatgttcc
     1321 agggccaaaa agacatgaac ggcctttaac tcaactgacc atttggtcag aaatttaaaa
     1381 tagcgaatga tgcgatcttc ctgggaaaga ggaagtcgcc tgagttctgc caggagattg
     1441 gcaaacgggt ctgaagtttt tgcctttgcc ttgagttccg caaggtcttc gccttcctgg
     1501 taccgttcat agatctcaga ctcagcatac accatattct gtaaaacttg acttttcgtc
     1561 ttgactggtt tgatgaccct tttttgcagc cgccggaaca tacgggcacc ctctgttcct
     1621 tgcgcaacat tgatgaccgt cagattcaca gattgccagg cgattttgcg ggaaagtgtc
     1681 tgactttgac ctcggaaaat ataatcggca agcttctgag cccggtctaa atcaaaatcg
     1741 tcaaattcaa gttttgtttg atcggaaatc ttattgattg gcatagattt gcttccgttt
     1801 ggtcagatcc gtgcaacgac ccaactgtaa gaactggttg tggcaaaaca tctgtttctg
     1861 atggtcaacc agaactgaac tccctcaact tctcactgca ggggagaggg gactcccgtt
     1921 ttataagggc tggctgacga ttgtgtgacc aaacttgtcg gtgcaagttt ggttggtgta
     1981 cctgaatttc aggtgtacaa ccctcgtgcc gagtttccgg tatctgcctg ttctccaaaa
     2041 tacagcagag aacaggctcc taacaatcca aaaattcatc aatgaaaatg aggtgttgaa
     2101 catgaaacaa catgaagaag cacaaggtta cgaactcaca ccgcgaacag tgacctcgct
     2161 ggttgccatt tcgacgattc cgaccacggc gtcgtctgca gacggagtga taaaagggaa
     2221 catcctttca cgagtggagg cagttcgcca gcaacaaggt ggttccgtgg gagagagctg
     2281 cccctggagc gcctcctggc ctgactaaaa ctcagattgt gtcacctatc caccggtgaa
     2341 gacgacaaga acccctggtt tcattggcaa tgaatctgtt tgggtggttt gccagactgg
     2401 cagataatgg ccaatgaaac cgggcctttc aaaaccaatg acgattccaa tgacgctgaa
     2461 caaagggctc aaactccgac ttccggctga catgccggtt gaatcagtga cgattaactc
     2521 tggaacgtac cttgtcccca gccagggtaa cgaaccccgg tgcattctgc caaagccgga
     2581 ttggcgacag cttaccctgg aagaatatga tcggctggta tcagttcaag atgtgccgct
     2641 ccaggaatca gtttcggtaa tcaatgttga tgagcacgtg cgcaccatat ttgaagatca
     2701 catcgcccgg cagttgtttg gccaacctca gttggcccct gatcaagcga aagcggtgtt
     2761 agaagggttt gctcagcagg ccgcccaatc attacaaatc gccggttggt cggtccggcc
     2821 cgtcgtttgt aatgcgacca tcaatccgcc aaacttgcct tcgacagcct ttgacttcga
     2881 acatggctct ttggtcggac tccatttcga caatcgggaa actaactttc acctc
//
LOCUS       DAOQDO010000586         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27325_length_2934_cov_4.334491, whole genome shotgun sequence.
ACCESSION   DAOQDO010000586 DAOQDO010000000
VERSION     DAOQDO010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27325_length_2934_cov_4.334491"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKZ53_10330"
     CDS             complement(<1..184)
                     /locus_tag="PKZ53_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40876.1"
                     /translation="MFYPALICTRRDSRRAEIMAALRFYKNQQGAATYESFDLPVYFV
                     AGIGVSSLFWGFGAMCR"
     gene            complement(342..1106)
                     /locus_tag="PKZ53_10335"
     CDS             complement(342..1106)
                     /locus_tag="PKZ53_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNJ40877.1"
                     /translation="MKQYSFLNSDRLQQIHLTTMRGQQPCPWVLFHQLITPDGFEQLC
                     QDFPSLDWFEKHQDQPRKSNQRPHNRYYLAYQQSFFSYQKARTSAFIKHSQLALSWQT
                     LIEELQQPQYLQFLTHFFGTSAFQLQFSWHIGLTGSEVSPHCDWVGKLGTHLFYFNTS
                     QEWSESWGGELIFLSNKQTDVPNPDFEDFETHQALPFLDNRSTLFQNNPSAWHGVRPL
                     TCPEGNYRRLFNVIATRLSRPERLLQQVQRLIPGRG"
     gene            complement(1122..1937)
                     /locus_tag="PKZ53_10340"
     CDS             complement(1122..1937)
                     /locus_tag="PKZ53_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40878.1"
                     /translation="MQTLLDGIYPHQILTDPFPHLVVTDALPETLHQQLLATYPPLEV
                     LTSGAQFTSNQRFNLMGSEVIHHPAIDPVWREFVKLHISELFLRQMIQLFGTHLRAQY
                     PDFEKTAGSLDLLRPGLRLVDDFQTADVLLDAQLAVNTPVTDMPSSVRRGHVDWPNKL
                     FVGLYYLRHPGDTSQGGELELYRFAGSRHRMHKASIEDQYIEVVKTIPYRSNTLVMFL
                     NGIDALHGVTTRYLTPFPRCFFNLVAEVERPLFNLDQYQSRWWKRAKAMILFQ"
     gene            complement(1996..>2934)
                     /locus_tag="PKZ53_10345"
     CDS             complement(1996..>2934)
                     /locus_tag="PKZ53_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40879.1"
                     /translation="FLHLESGEMSESPMSLFENIEPGTRNPEPGTRKSFLSTLSPSQI
                     IHEPFPHIVVDKMLPEPLCQQLIREFPRLDVFTKGQPYGDNQKRYYHAIHALAEGHLS
                     PAWKTMVSDFLFQSTWVEAYRLFRSAILTEYPDFEAQYGNPEDFRIGTRFRDDPAACD
                     MLLNSFLLIHTPIHGQACVERGPHYKMFKTLYVGYLFLRPEEDDSEGGDLELFSIKPG
                     AKIQLNQRHTVDRELLQLEKVIPYRANTFVLFLNTSRSIQGVSLRSPTRFPMMHLHFN
                     AYFRKPLFHVKDKPGTVARDFLERNIKRVTRLVSNK"
BASE COUNT          767 a          676 c          756 g          735 t
ORIGIN      
        1 gacggcacat tgcaccgaag ccccaaaaca ggctggaaac accgatacca gcaacaaagt
       61 acacagggag atcaaaactt tcataggtgg cagctccttg ttgattttta tagaatctca
      121 aagccgccat tatttctgct cgtctgctat cgcgtcttgt acaaatcaaa gccgggtaga
      181 acaaatcaaa gtttgcatac cagtttgtcg tcagtcgtca gtggtcagtc gtcaggtgat
      241 aagtgttggt ttttttaact tgtgtcaggt ctatctgatt ctggttaagt catttatgag
      301 gtgttctttt caaaaactct tcaaccttga gcccggcttt ttcagcccct gcctgggatc
      361 agtcgttgaa cctgttggag caatcgttct ggacgactca accgggttgc aatcacatta
      421 aacagccgcc gatagttgcc ttccgggcag gtcaacgggc gcaccccatg ccaggcagaa
      481 gggttatttt gaaacagcgt gctccggtta tccaaaaagg gaagagcctg atgggtttca
      541 aaatcctcaa agtctgggtt tggaacatcg gtttgtttat tgctgagaaa gataagctct
      601 ccgccccagc tttctgacca ctcctgactg gtgttgaaat aaaacaaatg ggttccaagt
      661 tttccaaccc agtcacaatg cggagaaact tcagaaccag tcaggccgat atgccaggaa
      721 aattgaagtt gaaaggctga agttccaaag aagtgcgtca gaaattgcag gtactgaggc
      781 tgttgaagct cctcaatcaa tgtctgccag ctcaaagcaa gttgactatg tttgatgaag
      841 gcagaagtcc gagctttttg ataggaaaaa aacgattgct ggtaggccag ataatagcgg
      901 ttgtgtgggc gttgattaga tttacgtggt tgatcctggt gtttttcaaa ccagtcgagc
      961 gagggaaaat cctgacacag ttgttcaaac ccatctggag taatgagttg gtgaaataaa
     1021 acccaggggc agggctgttg cccgcgcatt gttgtcagat gaatctgctg caaacggtct
     1081 gaattcagaa atgaatactg cttcacggca gtctcccctg gtcattgaaa aaggatcatc
     1141 gctttggccc gtttccacca gcgtgactgg tactggtcca gattgaacaa tgggcgctcg
     1201 acttcagcga ccagattgaa aaagcagcgt ggaaatggcg tcaggtaccg ggttgttacc
     1261 ccatgaagtg catcaatccc gttcaaaaac atgaccagcg tgttgctgcg gtacgggatg
     1321 gttttcacca cctcgatgta ctgatcttca attgacgctt tgtgcattcg atgacggctt
     1381 ccagcaaacc gatacagttc gagttcacca ccctgtgagg tgtctcccgg atgccggagg
     1441 taatacagac cgacaaagag cttattgggc caatccacgt gcccccgacg cactgacgat
     1501 ggcatatcgg tgaccggggt gttgaccgca agctgggcat cgagcagcac atctgccgtt
     1561 tgaaaatcat ccaccagccg taaccctggc cggagcaagt ccagtgagcc agccgttttc
     1621 tcaaaatctg gatattgtgc ccggagatga gttccaaaaa gctggatcat ctgacgcaaa
     1681 aagagttctg agatgtggag cttcacaaat tcccgccaca ccggatcaat cgccggatga
     1741 tgtatcacct ctgatcccat caagttaaac cgctgattgc tcgtaaactg agcaccagac
     1801 gtcagaacct caagcggcgg ataggtcgcc agcagttgct gatgcagcgt ttcaggcaag
     1861 gcatctgtca ccaccagatg tggaaacggg tcggttaaaa tctgatgtgg gtaaatccca
     1921 tccagaaggg tttgcatacg attggaacgt tgaaggttgg tatctcctta actgaggtaa
     1981 cgaactgcgg agcttctatt tatttgaaac taaacgggtt actcgcttga tattccgttc
     2041 aagaaaatcc ctggccacgg taccgggttt gtctttcacg tgaaacaatg gttttcgaaa
     2101 ataggcatta aagtgaaggt gcatcattgg aaacctggtc ggcgaccgca gtgacacacc
     2161 ctgaatggag cgcgaggtat ttaaaaacag gacaaatgta ttggcacgat acggaataac
     2221 tttttcaagc tgaagcagtt cccggtcaac cgtgtggcgc tggttaagct ggatttttgc
     2281 cccaggtttg atcgaaaaca attccaggtc gccaccttcc gaatcatcct cctctggacg
     2341 gagaaacagg tacccgacat agagggtttt aaacattttg taatgtgggc cgcgttcaac
     2401 gcacgcctgt ccatgaattg gggtgtgaat cagcaggaac gaattcaaca acatatcgca
     2461 ggctgccggg tcatcgcgaa atcgggtccc aatacgaaag tcctcaggat ttccatactg
     2521 tgcttcgaag tctggatatt cagttaaaat tgcggatcga aagaggcgat acgcctccac
     2581 ccaggtggat tggaacagga aatctgaaac catggttttc caggctggtg aaaggtgacc
     2641 ttcagccagg gcatgaatcg cgtggtaata ccgtttctgg ttgtctccat agggttggcc
     2701 tttggtaaac acatccaacc gtggaaattc gcggatcaat tgctgacaaa gcggctctgg
     2761 gagcatcttg tccaccacga tgtgtggaaa cggttcgtga atgatctgac tgggtgaaag
     2821 tgttgagagg aaggattttc gggttccggg ttccgggttc cgggttccgg gctctatgtt
     2881 ttcaaataga ctcattggtg attcactcat ttcgccagac tccagatgga ggaa
//
LOCUS       DAOQDO010000587         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27327_length_2934_cov_3.452240, whole genome shotgun sequence.
ACCESSION   DAOQDO010000587 DAOQDO010000000
VERSION     DAOQDO010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27327_length_2934_cov_3.452240"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..699
                     /locus_tag="PKZ53_10350"
     CDS             1..699
                     /locus_tag="PKZ53_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40880.1"
                     /translation="MDDAQRRIGELERENAALRAELDRFCQELRRVVGNLGEIIQSGN
                     TITVRLKSDILFPEGVWLLTVDRDLNKDARPRLAQLALLLQMFYSNSQFAFVGHTDTV
                     DEPDYNQWLSEQRALEVMRFFYIQRAQFMDSGDPLYGDYQQKISVSDQLLDNRLFPEW
                     LSSRRKGGLGADRRKQEERAVLLDQLRDVVRGMGETQLAVPTADNTTEARNRRVEIQI
                     TLPPQSALPYCNSR"
     gene            994..2829
                     /locus_tag="PKZ53_10355"
     CDS             994..2829
                     /locus_tag="PKZ53_10355"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ40881.1"
                     /translation="MIAGHASAYLISIVLIFQMLFGLGGSTDRKPISGPTGAAVITED
                     TIRKHVVYLASDELSGRLPGTPGAEKAARYIADNFKQAGLKPSGTKGFFQEFPFVSKL
                     KLGKKNQLAMTLAGTKSAAKLNQDFVPVGFSSPGMVSGDVVFAGFGISAAEDAKYDDY
                     AGLDVKGKLVLVLPYSPTGANPHAKFGAYSTLRYKAATAKSKGATGILIIAEDEDFKS
                     APSARLVYDESYGDSGIPAVVVSRALGNQFLKSKNQDVAGLEKRLAETLTPEAFALPQ
                     TQVVLQTEVIKERQTGRNVVGWLEGSDPQLKNEIVVIGAHYDHLGLGGANSLAPKEGE
                     IHNGADDNASGTAGVIELARAFASAPERPKRSLMFMAFSAEEKGLLGSAYFTENPMKP
                     LSQIVTMINMDMVGRLRDNNLMIQGMGTATEWKGIIEKVNQTASFNLKLQDDGFGPSD
                     HSSFYRKQIPVLFFFTGNHEDYHRPSDDADKINYEGERNILSFVFHTATAISNQPVKP
                     TYQKAKSDDQGRRGGGFRVYVGSIPDYAAEVEGVKLSGVREGSPAEKAGIKAGDVIVK
                     MAGRDVKNVYDYTYVLQELKPDQEVEVVVLRGTERVTLKLTPGKR"
BASE COUNT          755 a          706 c          800 g          673 t
ORIGIN      
        1 ctggatgatg cccagcgtcg cattggagag ctggaacgtg aaaatgccgc cctgcgtgcc
       61 gaactggatc gcttctgcca ggaactgcgc cgggtggttg gaaatctggg tgaaatcatt
      121 caatcgggaa acaccattac ggttcgcctc aaatcagaca ttctgttccc cgaaggcgtc
      181 tggctgctga cggttgaccg cgatttgaac aaagatgcgc ggccacgatt ggcccagctc
      241 gcactcttgc tccagatgtt ttattccaat tcacaatttg cgtttgtcgg ccacaccgac
      301 acggtggatg aacctgatta taaccagtgg ctttcagaac aacgggccct ggaagtgatg
      361 cggtttttct acatccagcg ggcacagttt atggactctg gagacccgct gtatggcgac
      421 tatcagcaga aaatctcagt ttcggatcag ttgcttgata accggctgtt ccccgaatgg
      481 ttgtccagtc gccgcaaagg tggccttggc gctgaccgcc gcaaacagga agaacgggcg
      541 gtcttgcttg atcaattgcg ggatgtcgtc cgtggtatgg gcgaaactca actggccgtt
      601 ccaactgctg acaacacaac cgaagctcgg aaccggcggg ttgaaatcca gattacactg
      661 cctccgcaat cggccttgcc atactgcaac agccggtaat cactccagga gatggaggat
      721 tggggatgga ggatagcgtt taaggataat ttgtctaatt catctttggg gtttggtggg
      781 ttttgtttta aagttgcccg ccaaacccca atgtcatttt taaagcaggg ttcacaaaag
      841 gacttaaagg actcaaattc gaaaactctg aaccctgaca agctgaccaa ctgacaaggt
      901 gacaaggtga caaggtgatc gggtgatttt tttcatccct catccctcat ccctcatccc
      961 tttagaaacc ctgaaccctg aaaggatcaa gctatgattg caggtcatgc gtcggcgtat
     1021 ctcatttcga tagtgttgat tttccaaatg ctttttggtt tgggaggttc cactgaccga
     1081 aaaccaattt ccgggccaac gggcgcggcg gttatcaccg aagataccat tcgaaaacac
     1141 gtggtatatc tggcttcgga cgagttaagt ggacggctgc cagggacacc tggcgccgaa
     1201 aaagcagccc gctacattgc cgacaatttc aaacaggcag gactcaaacc atctggtacc
     1261 aaagggtttt tccaggaatt cccgtttgtg tcaaaactca aactgggcaa gaaaaatcaa
     1321 ctggcgatga cgctcgcggg cacaaaatca gcagctaaat tgaatcagga ttttgttccg
     1381 gttgggtttt cgtctccggg aatggtcagc ggagacgttg tgtttgctgg gtttggcatc
     1441 agtgccgccg aagatgcaaa gtatgacgac tatgccggtc tcgatgtcaa aggcaaactg
     1501 gtgctggtac tgccttacag cccgactggc gccaatccac acgccaaatt tggggcctat
     1561 tcgacgttgc gctataaggc tgccacggca aaatccaaag gtgccacagg tattttgatc
     1621 attgcggaag acgaagattt caaaagtgcg ccatcagccc ggctggtcta tgatgaaagc
     1681 tatggcgatt cggggattcc ggcggtggtt gtgagtcggg cattgggcaa tcagttcctg
     1741 aagtcaaaga atcaggatgt ggcgggactt gaaaaacgcc tggccgaaac gctgacccct
     1801 gaagcctttg ccctccctca gacacaggtt gtcctccaaa ccgaagtcat caaagaacgc
     1861 cagactggtc gaaacgtagt tggctggctt gaaggcagcg acccacaatt gaaaaatgaa
     1921 atcgtggtga ttggggcgca ctatgaccat ctcggtttgg gtggcgccaa ttcactggcg
     1981 ccgaaagaag gcgaaatcca taacggtgcg gatgataacg cttctgggac ggctggtgtg
     2041 atcgaactgg ctcgggcttt tgcttcggct cccgaacgcc cgaaacgcag cctgatgttt
     2101 atggcgttct ccgccgaaga aaagggcttg cttgggtcag cctactttac cgaaaacccg
     2161 atgaagccgc tttcacaaat cgtgactatg atcaacatgg atatggtggg ccggttacgc
     2221 gacaacaacc tgatgattca gggaatgggc accgctaccg aatggaaggg aattattgag
     2281 aaggtcaacc agaccgccag ttttaacctg aaattacagg atgatggatt tgggccaagc
     2341 gaccattctt cattttatcg caagcaaatt ccggtgctgt ttttctttac cggaaaccac
     2401 gaagactatc accgcccatc agacgatgcc gacaagatca actatgaagg cgaacgcaac
     2461 attctgagct ttgttttcca cacggcgacg gctatctcta accagcccgt caaaccgacg
     2521 taccaaaaag cgaaaagcga tgatcaggga cggcgtggcg gtgggttccg agtgtatgtt
     2581 ggttcgattc cagactatgc ggctgaagtc gaaggcgtga aactgtcggg ggttcgtgaa
     2641 ggaagtccag cagaaaaagc tggtatcaaa gccggcgatg tgattgtcaa aatggccgga
     2701 cgggatgtga aaaacgtgta tgactacacg tacgtcttgc aggaactcaa acccgaccag
     2761 gaagttgaag ttgtggttct gcgtggcaca gaacgagtga ccctgaaatt aactccagga
     2821 aaacgataat cagatgaaga atgaagaatg aagaatgaag aatgaagaat gaaaaaacca
     2881 attccgggtt ccgggttccg ggttccgggt tccgggttcc gggttccggg ttcc
//
LOCUS       DAOQDO010000588         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27364_length_2932_cov_3.481752, whole genome shotgun sequence.
ACCESSION   DAOQDO010000588 DAOQDO010000000
VERSION     DAOQDO010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27364_length_2932_cov_3.481752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PKZ53_10360"
     CDS             complement(<1..1375)
                     /locus_tag="PKZ53_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ40882.1"
                     /translation="MIEFAPPSTPHPLPQQPLVMVVDDDFQIQRLLQITLEREGYKVI
                     LAGNGEDALMLFQQMRPDLVLLDIMMPGLDGIETCRRLRQFPGAEKIPILMLTALADE
                     AAVENAFAAEATDFLIKPIHFTVLRHRVRRLVKARQAEIQLHNTEARMHSIVFHALDA
                     IINFTWEGTVEFLNPAAERMFGLSGSETRGVAIQKLLDIEDPKLLIHFGIPLESQGIR
                     QDGSTFPAEISMSRFQTDHYDKFTVIVRDITERKQAEVALRTSHQIVQKTIDALKAHI
                     AILDDKGTILSVNAAWRDFADSNQMVGNYGIGVNYLAVCDSACGPNADEASSVATGIR
                     NVLTGKQREFVLEYPCHSPTRQRWYSLLVTRITDVSPAIGTRLVVMHQDTTLRRLTED
                     ALRQTYRQSQERIRQAEIEWVATVDAISDMILLVDQSGHIRRCNSAVVRLLDLPYTRL
                     IGRLIDEI"
     gene            complement(1727..2593)
                     /locus_tag="PKZ53_10365"
     CDS             complement(1727..2593)
                     /locus_tag="PKZ53_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ40883.1"
                     /translation="MKTLGLTFAGGGNRAFYQLGLMNQWGSQIWPHVGAVAACSAGAC
                     VVTFILSGRQHEAGEFWKQRREGVVKNFVWSNLLKGQRPTPHAPIYRDTLLHAFAEGG
                     LARVQNQPFPIWVLTTKILPWLPALATVMLGLSAYSLEKALRKEMIHPSFGRYLGFTP
                     FAYDARQCRTPAELADLILASSATPPFTPLGKFSGHRLLDGGIIDNAPAFLLDQDGSI
                     EQTVVFLTRPYPVHVLGIQGPRLYIAPTTDTPVDRWDYTRPELIEATIAMGESEAQLH
                     EPALRQFLKHLV"
BASE COUNT          746 a          740 c          757 g          689 t
ORIGIN      
        1 agatttcatc aatcaagcga ccaatcaggc gcgtgtaggg aagatcgagt aacctgacca
       61 cggcactatt gcaccgccgg atgtgtccgc tctgatcaac cagcaaaatc atgtcggaaa
      121 ttgcatccac ggtggcaacc cattcaattt cagcctgccg gatgcgttcc tgagattgcc
      181 ggtaggtctg gcggagggcg tcttcggtca gtcgtcgcag ggttgtatcc tggtgcatga
      241 ccaccagccg tgtcccaatc gcaggtgaga cgtcggtgat cctggtcacc agcaggctat
      301 accatcgctg tcgggttggg ctatgacagg ggtattccag gacaaattca cgttgtttgc
      361 cagtcaaaac attccggatc ccggttgcca ctgacgaggc ttcgtccgca tttggcccac
      421 aggcagaatc acacaccgcc aggtaattga ccccaatccc atagtttccc accatctggt
      481 tggaatcagc aaagtcacgc caggccgcat tgactgataa aatcgtgccc ttgtcatcca
      541 aaatggcaat gtgggctttg agggcatcaa tggtcttttg cacgatttga tgtgaggtcc
      601 ggagcgccac ctcagcctgt ttgcgttcag taatgtcgcg aacaatcacc gtaaatttgt
      661 cataatggtc ggtttgaaac cggctcattg aaatttcagc cggaaatgtc gagccatctt
      721 gtcggatccc ctgggattcg agcggaattc caaagtgaat caacagtttc gggtcttcga
      781 tgtccagcag tttctggatc gcaacccccc tggtttctga tccggataag ccaaacatcc
      841 gttcggcagc cgggttcaaa aattcaacag tgccctccca ggtaaaattg ataatcgcat
      901 ccagggcatg aaagacaatc gagtgcatcc gcgcttcggt attatgcaac tggatctcag
      961 cctgacgggc tttgaccagt cgccgaactc gatgccggag cacggtaaag tgaatcggtt
     1021 tgatcaaaaa gtcagtcgct tcagccgcaa aggcattttc cacggctgct tcgtcagcta
     1081 aagctgtcag catcaaaatt ggaatttttt ccgcccctgg aaactgtctc aatcgccgac
     1141 aggtctcgat accgtcgagt ccaggcatca taatatccag caaaaccagg tccggtcgca
     1201 tttgctgaaa caacatcagg gcatcttcgc cattcccagc cagaatgacc ttatagcctt
     1261 ctcgttcaag cgtaatttgg aggagccgct ggatttgaaa gtcatcgtcc acgaccatca
     1321 ccagcggttg ctggggcaaa gggtggggtg ttgagggtgg ggcaaactca atcatatgga
     1381 gtacattcct tggctttgga aggcggcaat acgggatgag aggttttacc cttatcgttt
     1441 caaccggatt gaaaaaatgt tggggtttaa tagggctgag ggtaccgggc caggggttga
     1501 gggaaataca taattttcaa ctgtttagcc tttttctcac gcgatcagat cattgaaatg
     1561 gagtgacata ccgaccgatc acccgtcacc gctctgtttc ttgccccggt ttgcaagacc
     1621 tgagaaaatg aagggcatca actttgcaaa cgactggttc tacaatagtt tcagtttgag
     1681 cgagtacaga ggatcgactg gatgaaccca aaaagaaaag cacgcactat accaaatgct
     1741 tgagaaactg acgaagagca ggttcgtgaa gctgcgcttc tgattcaccc atggcaatgg
     1801 tggcttcgat cagttcgggg cgagtgtaat cccaccggtc aaccggagtg tcggtcgttg
     1861 gtgcgatata taatcgcggt ccctggattc caaggacatg aaccggatag ggacgtgtca
     1921 agaaaacgac agtttgctca attgaaccgt cttgatccag taagaaggcg ggggcgttgt
     1981 caataatgcc cccatctagc agtcggtgac cactaaattt tccgagaggg gtaaagggcg
     2041 gggtggctga agatgccaga atcaagtcag ccagttcagc aggggtccgg cattgccggg
     2101 catcataggc aaaaggggta aaccccagat accggccaaa cgatgggtgg atcatttcct
     2161 tgcgcagggc tttttcaagc gaataggccg aaagacccaa cataacggtt gccagggctg
     2221 gcagccaggg caaaattttg gtggtcaaaa cccaaattgg aaagggttgg ttctgaactc
     2281 gcgctaatcc accttcagca aacgcatgca acagcgtatc gcggtaaatt ggagcatggg
     2341 gggttggtcg ctgccctttg agcaggtttg accagacaaa atttttgacc acgccttcgc
     2401 ggcgttgttt ccagaattca ccggcctcgt gctggcgacc acttaaaatg aacgttacga
     2461 cacaggcgcc ggcgctgcag gctgccacgg cacccacgtg tggccagatc tgtgatcccc
     2521 actggttcat cagacccagt tgataaaacg cacggtttcc accaccggca aaggtcaatc
     2581 caagagtttt catggtgtgc aatttgttct cgacctgctg aggaatcggt accaaataac
     2641 ttcatcaact tgaaacgcat atgtacgaag ccaaaatctc accacagagg acacagagga
     2701 acgcagaggg aaatcaatca gcttcaacct ctgtgttcct ctgtattctc tgtggtgaaa
     2761 aaatgatgaa gttattttat ttccaaccct tcattgatac cggttcgtca tcaaacaaaa
     2821 atcggcggac tccataactg ttggtacaaa ctcaactgtg attcctatct ggcggctgaa
     2881 acttcccgaa tccatccatc gcgccgggtt ttccaggctg gagtcaccga ag
//
LOCUS       DAOQDO010000589         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27389_length_2930_cov_4.680696, whole genome shotgun sequence.
ACCESSION   DAOQDO010000589 DAOQDO010000000
VERSION     DAOQDO010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27389_length_2930_cov_4.680696"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKZ53_10370"
     CDS             <1..764
                     /locus_tag="PKZ53_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40884.1"
                     /translation="QTRREHWRYYTGPSISIGTPESNDPNFLKNLPGSRWFFDQTQPV
                     TRENLIYRCLAPARYLNKLLFYHYGFDRVVYEFEVSNLLWKKFDFRTLASYPTLYFRA
                     WQFFHSSDSSPAAQFRLVAELRDRVAAETAHFSDYTVGLHIRRTDHVRAIANSTTEKF
                     IAAIQAEIDQHSSARFFLATDSPEEETTLRRLFPDRILTYQKRTLDRNSPEGIQDAVV
                     DLYCLARTQKILGSIGSSFSRTASRLGDCPLTMVD"
     gene            806..2515
                     /locus_tag="PKZ53_10375"
     CDS             806..2515
                     /locus_tag="PKZ53_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ40885.1"
                     /translation="MTQFTEYRGIEGINYFDQDRGLQLLLRDWVPEALREEIFASLQN
                     CAGHVTDRWFTLAQDGNRPEQLPRIVNFDRVGNRVDAIDFGLHTRQLRREVAQETGLF
                     TRTRNDLHHFALIYFLAHNGEASLTCPISCTDGLIRAIAAKGSEYLKATYLEPLCSAH
                     TPFAGAQFVTERVGGSDVGAIEMEARPNEDGTWSLTGDKWFCSNPDEFFAVAARPTGA
                     PAGTDGVALFMVPRVLPDGTQNRIVYHRLKDKLGTRSLPTAELVFDGATGWLIGEAHD
                     GFKTLMHYVLNTSRIHNAVNATGFLRRAFLEGRNYARQRTAFGQAIENYPLVQETLVT
                     LLERVWRYRLLTFKLISLVDEHGLSPAEAEQSMWQRFLVNLAKYRTAENLTASIHDAT
                     MLFGGNGIVEDFTILPRLVRDAMIIETWEGAHNTLCLQILRDAARSTLLDRWQVEIQR
                     VVDLWPTDFLAQTRTRFTAVLAQVKAVLDQKANRDRQWVAVHARRMVNTLGSLLEVAW
                     MVELAVRHQGEDATPAVLAAVAARDVFTSDHRFESLLPETLWPHVTTLIDETPFQMDV
                     SNI"
     gene            2657..>2930
                     /locus_tag="PKZ53_10380"
     CDS             2657..>2930
                     /locus_tag="PKZ53_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNJ40886.1"
                     /translation="MSARLLDGKLVGQKLKQDAAEKAAFLTERGVKPGLAAVLVGDNP
                     ASRTYVGSKVKTCAELGLYSEKIVLPVETTTAELLAVVHTLNERTDI"
BASE COUNT          640 a          748 c          804 g          738 t
ORIGIN      
        1 ggcagactcg acgggaacac tggcggtact ataccgggcc gagtattagc attgggactc
       61 ctgaatcaaa tgatcccaat tttttgaaaa acctgcctgg aagtcgctgg ttttttgatc
      121 aaacccagcc agttacccga gagaacctga tttatcggtg cttggcacca gcccggtacc
      181 ttaataagct gctgttttat cactatgggt ttgaccgggt tgtctatgaa tttgaagttt
      241 caaacttgct ctggaaaaag tttgattttc ggacactggc cagctaccca accctgtatt
      301 ttcgggcctg gcaattcttt cattcatcag atagttcccc ggcggcccag tttcggctgg
      361 tcgctgagct tcgagatcgg gttgcggctg aaaccgcgca ttttagcgac tacaccgtgg
      421 ggcttcatat tcgacgaact gatcacgtcc gggccattgc caatagcacc acggaaaagt
      481 ttattgccgc catccaggct gagattgacc aacattcgtc agcccgtttt tttctggcga
      541 ctgattcgcc agaggaagaa accaccttgc gccggttgtt tccagaccgg attctgacct
      601 atcaaaagcg cacgctggat cgaaattcgc cggaaggaat tcaggatgca gtggttgatc
      661 tctactgcct ggcccgaacc caaaaaatcc tcggaagcat cgggagttca ttttcacgta
      721 ccgcttcccg gcttggcgac tgtccattaa caatggttga ttgatttccg ggcgtaccgc
      781 agacgccttc tggtaaggtg aacttatgac acagtttact gagtatcgag ggattgaagg
      841 cattaactac tttgatcagg accggggtct tcagcttttg cttcgtgact gggtaccgga
      901 ggcgctccgg gaagaaatct ttgcgtcctt gcaaaactgt gccgggcacg tgaccgaccg
      961 ctggtttacg ctggcccagg acggaaaccg gcctgaacag ttgccgcgaa ttgtgaattt
     1021 tgatcgggtt ggaaatcggg ttgatgcgat tgattttggg ctgcacacgc gacagttgcg
     1081 gcgcgaagtg gcgcaggaaa caggactgtt tacccgaaca cgcaacgatt tacaccactt
     1141 tgcattgatc tattttcttg cccacaatgg cgaagcatct cttacctgtc cgataagctg
     1201 taccgacggg ctcatccggg ctattgccgc caaagggtcg gaatatttaa aagccaccta
     1261 cctggaacca ttgtgttcgg cgcacactcc gtttgcgggc gcccagtttg tcaccgaacg
     1321 agttggggga tctgacgttg gcgccattga gatggaagcc cggcccaatg aagacggcac
     1381 ctggtcgctc acgggtgata aatggttttg ttccaacccg gatgaatttt ttgccgtggc
     1441 ggctcgtcca accggtgcgc cggctggaac tgacggtgtg gccttgttta tggtgccgcg
     1501 tgttttgcct gatggtacgc agaatcggat tgtttaccat cggttaaaag ataaattagg
     1561 gactcgctca ctccccacgg ctgaactggt ctttgacggg gccactggct ggttgattgg
     1621 tgaagctcac gatggcttta aaaccttgat gcactatgtg ctcaatacct cgcgcattca
     1681 caacgccgtc aatgccactg gctttttgcg ccgggctttt ctggaaggcc gaaattatgc
     1741 tcgccagcga accgcttttg ggcaggcaat tgaaaactac ccgctggttc aggaaacact
     1801 ggtcacgctc cttgaacggg tctggcggta tcggttacta acgttcaaac tgatttcgct
     1861 ggtagatgaa catggacttt cgccagccga agctgaacaa tccatgtggc agcggtttct
     1921 ggtcaacctg gcgaaatatc ggaccgctga aaacctgacg gcttcgattc acgatgccac
     1981 tatgctgttt ggaggcaatg gcattgtcga agattttacg attttaccgc gtctggtgcg
     2041 tgatgcgatg attatcgaaa cctgggaagg tgcgcataac acattgtgtc tgcaaatctt
     2101 acgcgatgct gcccggtcaa cgttgcttga tcgctggcag gttgaaatcc agcgtgtggt
     2161 ggacctctgg ccaacggatt ttctggccca aaccagaact cgctttaccg ctgtgctggc
     2221 gcaggtgaaa gcggttctgg atcagaaagc caatcgagac cgtcagtggg tggctgtaca
     2281 tgcccgccga atggtgaaca cgcttggcag tctcctcgaa gtcgcctgga tggttgaact
     2341 tgccgtgagg catcagggtg aggatgccac tccagccgtg ctggccgcgg tggctgcccg
     2401 tgatgtcttt acgtctgacc accgctttga aagcctgctg ccggaaacgc tctggccgca
     2461 cgtgaccaca ctcattgacg aaacaccatt tcagatggac gtttcgaata tctgagggct
     2521 cagggcttgg ggttgaagaa atcgggctga agaagacggg ctcaggggcg tacacccgtt
     2581 ttcttccgcc ccgagcttgc gagtcttcag ccccaagccc tgagccctca ttcttcttcc
     2641 aaagggagtt ttctctgtgt ctgctcgatt gcttgatgga aaactggttg gtcaaaaact
     2701 caaacaggat gcggctgaaa aggccgcctt tttaactgaa cgtggggtaa aacctgggct
     2761 ggccgccgtg ctggttggtg ataatccggc ttcgcggaca tatgttggca gcaaggtcaa
     2821 aacctgtgct gaactgggtc tgtattcgga aaaaattgtc cttcccgtcg aaaccacgac
     2881 ggcggaatta ctggctgtgg ttcacacctt gaatgaacgc actgacatcc
//
LOCUS       DAOQDO010000590         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27460_length_2925_cov_3.053310, whole genome shotgun sequence.
ACCESSION   DAOQDO010000590 DAOQDO010000000
VERSION     DAOQDO010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27460_length_2925_cov_3.053310"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKZ53_10385"
     CDS             <1..827
                     /locus_tag="PKZ53_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ40887.1"
                     /translation="HGVQVEFDNISVRVSGHTILEKINVTIEPGSHVAIVGPSGAGKS
                     SLVGLLLGWHRVASGQIRIDRKPLEGSRLDKLRQVTAWVDPSVFIWNRPLLENLRYGT
                     SPEIIQPIGQIIDQANLHSVLETLPDGLQTSLGEGGALVSGGEGQRVRFGRALFRSGV
                     RLVILDEPFRGLDRQQRQTLLARARAWWNKSTLLCITHDVGETRDFDRVLVIEQGRLV
                     EDGNPRQLSQQAGSRYQALLEAEAEIRKGLWSSADWRHVWLEKGVVSEKKGEHPEP"
     gene            961..>2925
                     /locus_tag="PKZ53_10390"
     CDS             961..>2925
                     /locus_tag="PKZ53_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ40888.1"
                     /translation="MARSQTTEIEKLSWPVSKLSEAVEVLARKCGLPVQTREAPLLPE
                     QSLRADAAQLGWWIETLAQWLGLEAEPVETTYAEINRLLVSAGPAVLAIPSEVGPRIL
                     LLLESRGGVIWLITQKHTIEKRTVEELRPLLCQTLEAPFVTRIDSLLRDAGVSKRRMA
                     QARQTLLREQLSAARIGHCWMLRLHPGSPMTEHLRQALLHRYFLTLLGIYALQYLFLI
                     GSWWMIGRGMFQGQLDPAWVFAWALLLITMIPFRAWATWTQGLLSVGAGRILKQRLLY
                     GALQLAPEAIRHQGAGQLLGRVNESQAIEGLVLSSGFTSVSALVELVMALLVLTTSPG
                     GMLMAWLLAGWIVLTVILSWQYFRYRQEWTENRVQLTNDLVERMVGHRTRVAQELRQF
                     WHDGEDQAVERYHRLSRKLDHAGVRQSFVTRGWLILGIAGLGPAFVTGSATPASLAIG
                     LGGVLLAQRALAKIVSSIGQVTGVLIAWKQVSPFFQAAENTEVPSSFFALLSSRKLES
                     VPQGQMVLEADDLVFRYREYSTPVVKECTLHVRSGDRILLEGPSGSGKSTFASLLIGL
                     RSPESGLLLLHGIDRKTLGIESWRKRVASAPQFHENHILVGTFSFNLLLGKDWPPSPE
                     DLKEAETLCHELGLGDLLNRMPAGLLQMVGE"
BASE COUNT          633 a          775 c          841 g          676 t
ORIGIN      
        1 gccatggtgt gcaagttgaa tttgacaaca tcagtgttcg ggtttccgga cataccattc
       61 ttgaaaaaat caacgtcaca attgagcctg gcagccacgt tgctatcgtg gggccatcgg
      121 gtgccggcaa atccagcctt gtcggattgt tgttgggatg gcaccgggta gccagtggcc
      181 agattcgaat tgaccgcaaa ccgctcgaag gctcacgtct ggataaactc cgccaggtga
      241 ccgcctgggt tgacccatcg gtatttatct ggaatcgtcc gctcctggaa aatctgcggt
      301 acggcacctc accagaaatc attcaaccaa ttggccagat tattgatcag gccaatctgc
      361 acagtgtgtt ggaaaccttg ccggatggat tgcaaacatc attgggcgaa ggtggcgcgc
      421 tggtttcagg cggcgaagga caacgggttc ggtttggccg ggcactgttt cgttctggtg
      481 ttcgactcgt cattctggat gaaccatttc gtggccttga tcgccaacaa cgccagacct
      541 tgctggcccg cgcccgcgcc tggtggaaca agtcaacgct cctgtgcatt acccacgatg
      601 tcggtgaaac cagggacttt gaccgggtgc tggttattga acagggccgt ttggtcgaag
      661 acggaaatcc aaggcaactc agtcagcagg ccggctcgcg ctaccaggcc ctgctcgaag
      721 ccgaagccga aatccgcaaa ggcttgtggt ccagcgctga ctggcggcat gtgtggcttg
      781 aaaaaggcgt tgtttctgaa aaaaagggtg aacaccctga accctgaacc ccatcatacc
      841 aattagggtt ccgggtagcg ggttcagggt ttaaaaagac gaaagagaca aaaacgacga
      901 aaaggacaaa aattcgaaca ccctgaaccc tgaaccctga accctgaacc ctgaaccctc
      961 atggctcgct ctcaaaccac tgaaattgaa aaactaagct ggccggtctc aaaactgagc
     1021 gaggcagtcg aagtcctggc gcgaaaatgc gggctgccag tccagacccg cgaagcacca
     1081 ttgttgccag agcaatcgct gcgtgctgat gcggcccaac tggggtggtg gattgaaacg
     1141 ctggcccaat ggctgggact tgaagccgaa ccagtggaaa cgacctacgc cgaaatcaac
     1201 cggctgcttg tgagtgccgg accagcggtc ctggcgattc cgtctgaggt tgggccgcgc
     1261 attctgcttt tgttagagag tcgtggtggc gtcatctggc ttatcactca aaaacacacg
     1321 attgaaaagc gcacggtcga agaacttcgc ccgctgctgt gccagacgct ggaagcacca
     1381 tttgtaactc gcattgattc gctgctacgc gacgcgggtg tttccaaacg ccgaatggcc
     1441 caggcgcggc agaccttact tcgagaacaa ttgtcagccg cccggatcgg gcattgctgg
     1501 atgttgcggc tgcatccagg gtcaccaatg actgagcatc tacgtcaggc actcctgcac
     1561 cgatatttcc tgacattgct gggcatttat gcgctgcaat acctttttct gattggctcc
     1621 tggtggatga tcggacgtgg catgtttcaa ggccagctcg atccggcctg ggtgttcgcc
     1681 tgggcgttac ttttgattac catgattccc tttcgggcct gggcgacctg gacccaaggg
     1741 ctgttatccg ttggggcagg tcggattctc aaacaacggc tgctctatgg cgcacttcaa
     1801 ctggctccag aagccatccg acatcagggg gccggtcagt tgcttggtcg ggttaatgaa
     1861 tcacaggcca tcgaaggtct ggtcttaagc agcggattta ccagtgtttc agccctggtc
     1921 gaactggtga tggcgttgct ggtactgaca accagcccag gcgggatgct catggcgtgg
     1981 ttgctggcag ggtggattgt gctgacggtg attttaagct ggcagtattt tcgctatcgc
     2041 caggagtgga ccgaaaaccg ggttcagctc accaacgatc tggttgaacg catggtcggt
     2101 catcggaccc gcgtggcaca ggaactccgg cagttctggc acgatggcga agatcaggcg
     2161 gttgagcgct atcatcggtt gtcacgcaag ctggatcacg ctggtgttcg tcagtcgttt
     2221 gtgacccgtg gctggttgat tttgggcatt gccggactcg ggcccgcgtt tgtgactggt
     2281 tcagcaacac cagccagtct ggccattggg cttggtggtg tgctcctggc gcaacgggcg
     2341 ctggctaaaa ttgtttccag cattggacag gtcaccggcg tgctgattgc ctggaaacag
     2401 gtgtcaccgt tttttcaagc ggctgaaaac actgaagttc cctccagttt ctttgcttta
     2461 ctctccagtc gtaaactgga atcagttcca cagggtcaga tggtccttga agcagatgat
     2521 ctggtctttc gctatcgcga atattccact ccagttgtga aagagtgcac gctgcacgtt
     2581 cgttccggag atcgcatcct gctcgaagga ccgtccggga gcggcaaatc cacttttgct
     2641 tcccttttga tcggcctgag gtcgccagaa tccggattat tgctgctgca tgggattgat
     2701 cggaaaacgt tgggcatcga aagctggcgc aaacgggtcg cgtcagcccc tcaatttcac
     2761 gaaaaccata ttctggttgg taccttctcc tttaatctat tgcttgggaa agactggccg
     2821 ccttcgccag aggatctcaa agaagccgaa accctgtgcc acgaacttgg cctcggagac
     2881 ctgctcaacc ggatgccggc aggactcctg caaatggtcg gggaa
//
LOCUS       DAOQDO010000591         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27477_length_2924_cov_2.800976, whole genome shotgun sequence.
ACCESSION   DAOQDO010000591 DAOQDO010000000
VERSION     DAOQDO010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27477_length_2924_cov_2.800976"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..1408
                     /locus_tag="PKZ53_10395"
     CDS             443..1408
                     /locus_tag="PKZ53_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNJ40889.1"
                     /translation="MELTHLKTFRAVAETLNFTRAAERLHLTQSAVSHQIQMLEAELG
                     EPLFIRAKRGVKLSQAGKTALEYAERILNEAEELKERIRGTEHVPIGRVRVAAATQAF
                     VSLFAPLFESFMRTQGGIELLFRTTVSTHQTIEDILNGACDVGFASLPVSSPALQVTR
                     LFEDELVLVVGGNHRLRKKTTASVADVQRERLILFERGASIRRATDEFFAESGIRPKL
                     ALESNDTYFVKLMVAHGWGISLLPSWAVREEVAAGKLAQLKISGHRLRRVVNMVSLGR
                     FQSSATRTFLDFILEHKDTLQQMANCQEEVRSEDVKERRSEIVQP"
     gene            complement(1500..>2924)
                     /locus_tag="PKZ53_10400"
     CDS             complement(1500..>2924)
                     /locus_tag="PKZ53_10400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNJ40890.1"
                     /translation="HVANMFQLLGKDQKTAASHAKIVMAIETKLAEASMTQVELRDPK
                     ATYNLKNLAELKKLTPNFSWPAYFKAINAPGVKEVNVGQLKFFQAVNTLLTEIPIPEW
                     KIYLHWHLVSEASPFLSSKFVDEHFAFYSAYLEGKKENQPRWKRVVGMTDTALGEALG
                     QMYVEKYFSAQAKQRVLEMVANIKAAFQEKLSGLPWMSPQTRKQAIAKLNKIVTKIGY
                     PDKWQIYKGLEVSRESYLANIMASQQFEFARQLRKIGKPVDRTEWGITPPTVNAYYDP
                     LMNEIVFPAGILQPPFFDPNADDAVNYGGMGAVIGHEIIHGFDDQGRQFDKDGNLKDW
                     WTSDDETKFIERATCVEQQFSGYEVEKGVSVNGKLVLGESIADLGGLTISFAALQRAM
                     AGKPRPAEIDGFSPEQRFFLGWAQVWRTNMRPEFARYMVQVDPHPPAKFRVNGPLSNM
                     PEFTKAFGCKSGESMVRASQCQIW"
BASE COUNT          684 a          706 c          743 g          791 t
ORIGIN      
        1 cacatattcc aggcgaaatg gaattggcat ctgttcctcc ttttggctct cccacacgac
       61 agttgaactg tttctgccgg cagtggactg tttcaggcgg tatgccctga aacaaagccg
      121 caagacctag ccgtcttacg ggtggcctga gccaggccag gcatccgaac cagagttcga
      181 atggaatcag gaatgaggcc cgagattgtt ttagagttct ttcaaagtgt cggcacattg
      241 gctttcattt tgaatcaatc ccaagcactc ttgtcacgac agttcaggtg aacctgatct
      301 atctcacggt gattacattt tgttgttttg gaaatgcaaa tcgagcgtga tcatagcggt
      361 tcacctgcga tgcgtccaat tgattgttct aattcaatca atcaaaaatt tcgatgtctg
      421 gaacctatac caggaaaagc agatggagtt aacacacctc aaaacctttc gtgctgttgc
      481 cgaaactttg aactttaccc gggcggcgga gcgcctgcat ttaacgcaat cagccgtcag
      541 ccatcaaatc cagatgctgg aagctgagct tggcgagccg ctctttatcc gtgcgaaacg
      601 cggggtgaag ctatcacagg cgggaaaaac agcgcttgag tatgctgagc ggattttaaa
      661 tgaagccgaa gaactcaaag aacgcatccg cggaacagaa catgtcccga ttgggagagt
      721 ccgcgtggca gccgcaaccc aggcgtttgt ctcattgttt gctccactgt ttgaatcgtt
      781 tatgcgcacc cagggcggga ttgaattgct ctttcgaacc acagtcagca cccatcagac
      841 catcgaagac atcctcaatg gcgcctgtga cgttggtttt gcctcgctcc cggtgtcttc
      901 cccggcgctg caggtgacgc ggctttttga agatgaactg gtgcttgtcg ttggtgggaa
      961 tcaccggttg cgcaaaaaaa cgacggcttc agtggcggat gtgcagcgcg agcggttgat
     1021 tttgtttgaa cggggagctt cgattcggcg ggcgacggat gaattttttg ctgaaagcgg
     1081 aatccgccca aaactggcat tagaatcaaa tgatacgtac tttgtaaaac tgatggtggc
     1141 gcatggctgg gggatttctc tgctgccttc ctgggctgtt cgtgaagaag tcgccgccgg
     1201 aaaactggct caactcaaaa tttcaggcca tcggctgcga cgagtcgtga atatggtctc
     1261 attggggcgg tttcaatctt cagccacacg gacctttctg gattttattc tagaacataa
     1321 agataccttg caacagatgg ccaattgcca ggaagaggta aggagtgaag acgtgaaaga
     1381 gcgaaggagt gaaatcgttc agccttgatt tctgattttg atctatccct ctgaaaatca
     1441 aggattttga ctccttcact ccttcactct ttcattcctt tactcttttg ctgatgtggc
     1501 taccaaatct gacattgaga tgcccggacc attgattcgc ctgatttgca gccgaaggct
     1561 ttggtaaatt ccggcatgtt tgaaagcggg ccattgaccc ggaattttgc tggcgggtgc
     1621 ggatctacct ggaccatata gcgggcaaat tcgggacgca tattggttcg ccagacttga
     1681 gcccagccga ggaaaaaccg ttgttcaggt gaaaatccgt caatttcggc aggacgtggt
     1741 tttccagcca tcgcgcgttg gagggcggca aaggaaatgg tcagcccgcc aagatcggca
     1801 atactttcgc ctaaaaccag tttcccgttc acgctcacgc ctttttcgac ttcatagccc
     1861 gaaaactgct gttcgacaca ggtggctcgt tcgataaact tggtctcatc atcacttgtc
     1921 caccagtctt tcagattgcc gtctttgtca aactgacggc cctggtcatc aaagccgtgg
     1981 ataatttcgt ggccgatgac ggcgcccatt ccaccataat tgactgcatc atccgcgttt
     2041 ggatcaaaga acggaggctg caggattccg gctggaaaca cgatttcatt catcagcggg
     2101 tcataatagg cgttgaccgt cggaggtgta atcccccatt cggttcggtc tacgggtttc
     2161 ccgatttttc gcaactgacg ggcaaattca aattgctgcg aggccataat attggccaga
     2221 tatgattccc gagaaacttc cagtccttta tatatctgcc atttatcggg atacccaatc
     2281 ttggtgacaa ttttattgag tttggcaatc gcctgtttgc gggtttgtgg actcatccag
     2341 ggcaggcccg agagtttttc ttgaaaggcc gctttaatgt tggcaaccat ctccagcaca
     2401 cgttgctttg cctgggcact gaagtatttt tcgacataca tctggccaag tgcttcaccc
     2461 agagctgtgt ccgtcatccc taccactcgt ttccaccggg gctgattttc ttttttgcct
     2521 tcaaggtagg cactgtagaa agcaaaatgt tcatccacaa attttgagga taaaaacggt
     2581 gaggcttcac tcacgaggtg ccagtgcaaa tagattttcc attctggaat cggaatttca
     2641 gtcagcaggg tgttgaccgc ctggaaaaat ttgagttgac caacattgac ttctttgact
     2701 ccgggcgcat tgatcgcctt gaaataggcg ggccaggaga aattcggtgt caattttttc
     2761 aactcggcca ggtttttcag gttgtaggtc gctttagggt cgcgtaattc aacctgagtc
     2821 atcgaggctt cagccagttt ggtttcaatg gccatcacaa ttttagcgtg gctggctgca
     2881 gttttctggt ctttgccaag cagttggaac atattggcta cgtg
//
LOCUS       DAOQDO010000592         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27504_length_2922_cov_3.625392, whole genome shotgun sequence.
ACCESSION   DAOQDO010000592 DAOQDO010000000
VERSION     DAOQDO010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27504_length_2922_cov_3.625392"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKZ53_10405"
     CDS             <1..213
                     /locus_tag="PKZ53_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001908059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40891.1"
                     /translation="SSFFILNSSFFILHSSFFILNSSFFILNSQFFILHCPKPIFLKD
                     LLTFWTQNKLCQPSGLVWVLGALEDT"
     gene            complement(172..732)
                     /gene="modA"
                     /locus_tag="PKZ53_10410"
                     /pseudo
     CDS             complement(172..732)
                     /gene="modA"
                     /locus_tag="PKZ53_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
     gene            1050..1520
                     /gene="ribH"
                     /locus_tag="PKZ53_10415"
     CDS             1050..1520
                     /gene="ribH"
                     /locus_tag="PKZ53_10415"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNJ40892.1"
                     /translation="MPREIIGKLNADGMRCAIVVSRWNDLVVNRLLSGATDTLERLGG
                     SPDLWAVVRVPGSFEIPLVAKKLAVSGKWDTIICLGALIRGETPHFDYIAAEVTKGIA
                     AVSLESNVPITYGVLTADTVEQALNRAGLKAGNKGVEAAMAAVELFNLYKELSS"
     gene            1629..2093
                     /gene="nusB"
                     /locus_tag="PKZ53_10420"
     CDS             1629..2093
                     /gene="nusB"
                     /locus_tag="PKZ53_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNJ40893.1"
                     /translation="MGVRRRARECALQMLFQFDLAKPTLPELLKTYWEELNDTSVDAQ
                     EFATNLTLGVIAHLDEVDALIRRRTEHWRISRMAVVDRNLLRLAVYEFLYEAETPKTV
                     VINEALEIARRFSTYEATQFVNGILDGIKRDLEGTEEDPAPLTPQSSSATTA"
     gene            2118..>2922
                     /locus_tag="PKZ53_10425"
     CDS             2118..>2922
                     /locus_tag="PKZ53_10425"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ40894.1"
                     /translation="MSLAGTLEELPLADILQIISLGRRTGILTLLTADGRFTMSFRDG
                     LVVGATEAQGRPLGQVLIDSGFITAAQLAAALDLQISGDWKPLGAILVQQGVIIYDQL
                     EDVARRELERITRRIVQLQKGNFSFQVAEVPTYGSCRLNAQEMLLETARQHDELRHHQ
                     ALHSTSDPHPRKGYILIINPHGELVLPLRHALANLEVEVHAVSNLAPEALVAGFDELV
                     HSGLPALVIADLEEISPQPGGEPKLAWKCLKDIGERSITLITAEYPAQVT"
BASE COUNT          656 a          736 c          775 g          755 t
ORIGIN      
        1 tcttcattct tcattctcaa ttcttcattc ttcattcttc attcttcatt cttcattctc
       61 aattcttcat tcttcattct caattctcaa ttcttcattc ttcactgccc caaacccata
      121 tttctgaagg atttgctgac cttctggact caaaacaaac tctgccagcc gtctggactg
      181 gtctgggtgt tgggtgcgct tgaggataca taaggtctga ttgagcggtt gatagagtcc
      241 tgaatcaatc ggaaacacgt gtccttcggt gcggctgggt accagggatt tggccaccag
      301 acccacatcg gcattccccg tgctgacata ttggaacgcc tgactgatat tttccgccag
      361 aatcaacttt gattggactt gatcatagat tttcgcggct tgcagcgcct gaatcgctgc
      421 tttgccatag ggagcatggt ccgggttggc aatcgcaatg cggcggatgg ctgaattggt
      481 gagttcggcg agcgaggtta ctcgccgtgg agcatctgcc cgttgccaac cagccagata
      541 tccttgagca tagggagttg ttgatttttc taggaccaat ccttgtttac gaagttgatc
      601 ggtatagctt tgatcggcgg cgaaaaagag atcaaccggg gcaccttgct caatttggcg
      661 ggtgagcaag ccggtcgagc cgaaattgat ttccacgggt tgaccagttc tttgttgaaa
      721 gaaggtcgtc atttcctgca aggccggatt aagatcggcg gcagccgcaa cccgtaatgg
      781 ctcgggggcc cggttgtcgg gtgggagggt acatcccggc tccgcccagg ccaaaaccag
      841 tatgagaagg caaaggagat ttttcatagg tcggttgtca atcagggttt cgggaagctt
      901 gattgaaagt gtctttgcgt ggttcaaccg gctatgttat aaccaatgcc agcctttgac
      961 acctcgttcc tcacctgagg tagaaaagaa tcccaagttt gtcacaactt tcatactgat
     1021 ctggtttttc gccagccaag gaaaaatctg tgccacgtga aattatagga aaattaaatg
     1081 ctgatggcat gcggtgtgcc atcgtggtca gtcgctggaa tgatctggtt gtcaaccgat
     1141 tgctttcggg cgcaactgat acgctggaac gccttggagg atcacctgac ttatgggcgg
     1201 ttgtccgggt gccaggctcc tttgaaattc cgttggtcgc caaaaaactg gccgtcagtg
     1261 ggaagtggga taccattatt tgccttggtg cgttgattcg aggtgaaaca ccgcactttg
     1321 actatattgc cgcagaagtc acaaaaggaa tcgcagcggt ttcccttgag tcaaacgttc
     1381 cgattaccta tggggtctta acggctgaca ccgttgagca agccctcaat cgggctggtt
     1441 tgaaagctgg gaacaaaggg gttgaggctg cgatggccgc ggtggagtta ttcaacctct
     1501 acaaagaact ttcaagttag ggtgcagagt gcatctggaa ccgattccgc tggtgtgtat
     1561 gccgactcca gtgctttcca agttccagcc tctttaattt ttttcccgtc tgtaggattt
     1621 gaaatattat gggtgttcgt cgtagggcta gagagtgcgc cttacaaatg ctctttcaat
     1681 ttgatttagc aaaaccaact cttccagaac ttctcaaaac atattgggaa gaactcaatg
     1741 acaccagtgt tgatgcgcag gaatttgcca ctaacctcac cctgggggta atcgcccatc
     1801 ttgatgaggt tgatgcgttg attcgtcggc gcaccgaaca ctggcgaatt tcccggatgg
     1861 cggtggttga ccggaacctc cttcggttgg cggtctatga gtttttgtat gaggctgaga
     1921 cgccaaaaac tgttgtcatc aatgaagcac ttgaaattgc tcgccgcttc agtacctatg
     1981 aagcgaccca gtttgtcaat ggaatcctgg atggcatcaa gcgggactta gagggtactg
     2041 aagaggaccc ggcgccattg acgcctcaaa gttcgtctgc aacgaccgca taaacaacct
     2101 gtgatggaga ccaaccgatg agccttgccg ggacacttga ggaacttcct ctggcggaca
     2161 ttcttcaaat tatcagcctt ggccggcgca ccggaatcct tacgctgctc actgcagatg
     2221 gtcgcttcac gatgtccttt cgtgatggat tggtggtcgg ggcaaccgaa gcccaggggc
     2281 gtccactcgg gcaggtgctg attgattcgg ggtttattac ggcagctcaa ctggcagccg
     2341 ccctggactt gcaaatttcc ggtgattgga aaccgctcgg cgccatcctg gtccagcagg
     2401 gggtgattat ctatgaccag ttggaggatg ttgcccgccg tgaactggag cgcattaccc
     2461 gccgcatcgt ccagctccaa aaaggaaatt ttagttttca agtcgccgaa gttcccacct
     2521 atggcagttg ccgcctgaat gcccaggaaa tgctgctcga aaccgcccgg caacacgacg
     2581 aactccgtca tcatcaggcc ctccactcca ccagcgaccc ccatccccgc aaaggttaca
     2641 ttttgattat caatccccac ggggaactgg ttttgcctct gcgccacgcg ctggcgaatc
     2701 ttgaggtcga agtccatgcg gttagcaatc tggcacccga agccctggtc gccgggtttg
     2761 atgaactggt gcacagcggg ctacctgccc tggtgattgc cgatcttgag gaaatcagcc
     2821 ctcaacctgg cggagagcca aaactggcct ggaagtgttt aaaagatatt ggggagcgct
     2881 cgatcacctt gattacggct gaatacccag cccaggtgac tc
//
LOCUS       DAOQDO010000593         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27551_length_2919_cov_4.326816, whole genome shotgun sequence.
ACCESSION   DAOQDO010000593 DAOQDO010000000
VERSION     DAOQDO010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27551_length_2919_cov_4.326816"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PKZ53_10430"
     CDS             complement(<1..896)
                     /locus_tag="PKZ53_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40895.1"
                     /translation="MNARPARSTKSIQPWSILIALIALTVGLVGKYAPSSQATSRQTA
                     SQTQEASFTVEPNACTITVNSTAQEVTIAAPEGVRNSECTLGEAILVANGNPNITECP
                     CVGGAPVTIQLQAGMTYTLNTPATTLYGPTGLPAISSTIVIEGNGAVIERSSVGGTPN
                     FRLFSIAGTINGQPSLETNPNSIVPAPNAVLPAGNVTLRNVTLRNGLARGGNGGAKGG
                     GGLGSGGAIFNAGSLTLDASTVTSNQAVGGTGSTLCCSGGGGGMGGNGGNGFNAGGGG
                     GGMGGNGASTVEFDTGAGGGTVT"
     gene            complement(1141..>2919)
                     /locus_tag="PKZ53_10435"
     CDS             complement(1141..>2919)
                     /locus_tag="PKZ53_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40896.1"
                     /translation="RTAATWSHQQKLIAVDGAASDGFGNSVGISGELITIGARLDDEA
                     FADQGSAYVFERAGTIWSQDQKLVLPDGSDTDRFGTSVATQDGTILVGCPLDELGGSL
                     NVGSVRVFVRDCPQCSSPTGTVTGGATICAGGSATVSVVVSGGTPPYTVTLDNGGGTQ
                     TGASPLMFTVSPVANTTYAIQSGTDTGGCPIIGSGSAAITVTAAPTVTAGPDQTVCAG
                     SPQTLLAGSFGGSAVSCSWSGGGGAFSPNNTTTTATYTPTPAEVAAGTVTLTLTALTA
                     GGCSPVTDSMTITLTACPSAYALMVADTTNNRIQGFNGTNWSVLGPGTVGTGPGQFRV
                     PEAVTFDSRNWIYVADTGNNRIQWSTDGGATWAIFAALGSGFNQVRAPQGVAVDTAGN
                     LYVADTGNGRVLRFHGGVPGNGVVLSTQGTASHQVGSPRGLAIDSTFRLFVTDESNSR
                     ILRILSANTVTSATTGTLIATSGTALNKVKNPQGIAIDSAGTLYIADTGNSRILQWAN
                     ANPTSASTMALMGTGLGQVNRPEGITVTQFTSGPYAGGPLLVIGDTSNNRIQGRLLPT
                     GIWMLIGTPNGNGTGVGQFRAPSKIR"
BASE COUNT          619 a          892 c          773 g          635 t
ORIGIN      
        1 gtaaccgtgc cgcctccggc accggtatca aactccacgg ttgacgcccc gttgccgccc
       61 ataccgccac cgccaccgcc ggcattaaat ccattccctc cattgccacc catgccgccg
      121 ccgccaccag agcagcaaag agttgatccg gtccctccga cagcctgatt gctggtcacc
      181 gtactggcat caagcgtgag tgaacccgca ttgaaaatgg caccgccact ccccaaacca
      241 ccaccgcctt tggcccctcc attgccacct cgggccaaac cattgcgcaa ggtcacattc
      301 cgcaaggtta cattcccagc cggaagcacc gcatttggcg cgggaacaat tgaatttggg
      361 tttgtttcaa gtgagggttg gccattgatc gtgccggcaa tggagaaaag ccgaaaattt
      421 ggggttcctc caacactgct gcgctcaatc acagcgccgt tgccttcgat cacgatggtg
      481 cttgaaatag ccggaagccc tgttggacca tagagtgtcg tggccggagt gttcagtgtg
      541 taggtcatcc ccgcctgcaa ctggattgtc actggcgccc caccaacaca ggggcattcg
      601 gtaatgtttg gattgccatt ggccaccaga attgcctcac ccaacgtgca ttcgctgttg
      661 cgaacacctt ccggcgcggc aatggttact tcttgtgccg tgctattgac tgtaatcgtg
      721 caggcattgg gttccaccgt gaatgacgct tcctgcgtct ggctggcagt ttgtctggat
      781 gttgcctgtg acgacggagc gtactttccg acaaggccaa cagtcagggc aatcagagca
      841 atcaggatgc tccagggctg aatgcttttc gttgagcggg caggtcgtgc gttcatgaga
      901 atctcctcta tgtggctgcg aagttgaggt gacggcagga aagccactga ctcggattca
      961 attaatttga accatcaagc cgaagcggtt tccttcacgc cacctcatgc gcaaagccga
     1021 ggcgatttca ctcagtttat ttgatgaatc actcccgttg atgtggagtg cggcggcttg
     1081 acgccgcttt tccatcaacg tgccatttct gaaagctccg tcaagccgga gcactccaca
     1141 ttatctgatt ttgctcgggg ctcggaactg cccaaccccc gtaccgttgc catttggtgt
     1201 tccaatcagc atccagattc cggtcgggag caaccggccc tggatccgat tgtttgacgt
     1261 gtcgccaatc accagcaatg gtccgcccgc atatggaccg ctggtgaatt gtgtcacggt
     1321 gattccctct ggacgattga cttgccccaa tccggttccc atcagagcca tcgtggaagc
     1381 actggtcgga ttggcatttg cccattgcag gattcgcgaa ttcccggtgt cggcaatgta
     1441 gagtgtcccg gcactatcaa tcgcaattcc ctgtggattt ttgaccttgt tgagcgcggt
     1501 tcccgaggtg gcaataaggg tgccggtcgt ggcactcgtc acggtgttgg cactgagaat
     1561 gcggagaatg cgactgttac tttcatcggt tacaaacaac cggaatgtgc tgtcaatcgc
     1621 cagtccacgg ggacttccaa cctgatggct ggctgtgccc tgggttgaca acaccacacc
     1681 attgcctgga acaccaccat gaaagcgcag gacgcgacca ttgcccgtgt cggcaacata
     1741 taaattccca gccgtatcca ccgccacgcc ttgcggcgca cgcacctgat tgaacccgga
     1801 tccaagcgcg gcaaagattg cccaggtggc cccgccatcc gttgaccact ggatgcggtt
     1861 attccccgta tccgccacat agatccagtt tctcgaatca aaggtcaccg cttctggaac
     1921 ccggaattgt cccggtccag taccaacggt tccaggccca agcaccgacc agttcgtacc
     1981 gttaaagccc tggattcgat tattggtcgt gtcagccacc atcaaagcat aggctgaagg
     2041 acaggcggtc aaagtaatgg tcattgaatc cgtcaccggc gagcaccccc ctgccgtgag
     2101 cgccgtcaag gtcaaggtga cggttccagc ggcaacctca gccggagtcg gggtataggt
     2161 ggccgtggtg gtcgtattgt tgggtgaaaa ggccccgccg ccgccgctcc agctacaact
     2221 cacggcgcta ccgccaaacg aaccagccag caacgtttga ggactgcccg cacacaccgt
     2281 ctgatcgggc ccggcagtga ccgttggcgc ggcagtgact gtgattgcag cacttccgct
     2341 tccgatgatt gggcatccac cggtatctgt cccggattga atggcatagg tggtattggc
     2401 cacaggcgaa accgtgaaca tcagcgggct ggccccggtt tgtgttccac cgccattatc
     2461 aagcgtgacg gtatatggtg gcgttccccc agagaccacc acgctgacgg ttgcactgcc
     2521 tccggcacaa atcgttgccc cgccagtgac ggttccagtc ggacttgaac actgtggaca
     2581 atcccgaaca aacacccgga ccgatcccac attcaagctt ccgcccagtt catcgagggg
     2641 acatccaacc agaatcgttc cgtcctgcgt ggcaacggaa gttccaaacc gatcagtatc
     2701 actcccatct ggcagcacaa gtttctgatc ctggctccag atcgtaccgg cccgctcaaa
     2761 aacataggcc gaaccctgat cagcaaatgc ttcatcatcc aggcgtgctc cgatggtaat
     2821 gagttcgcca ctgatgccga ctgagtttcc aaaaccgtcg gaggcagcac catccacggc
     2881 aatcagcttc tgctggtgac tccaggtggc ggcagttcg
//
LOCUS       DAOQDO010000594         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27554_length_2919_cov_3.176676, whole genome shotgun sequence.
ACCESSION   DAOQDO010000594 DAOQDO010000000
VERSION     DAOQDO010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27554_length_2919_cov_3.176676"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKZ53_10440"
     CDS             complement(<1..223)
                     /locus_tag="PKZ53_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40897.1"
                     /translation="MTINFIDYRQETPRLIADECRPIFCLRVSPNHNTLSFLTRDNLP
                     GDPVRNSIWSNSGRACREAARGGNYGDPNE"
     gene            300..1118
                     /locus_tag="PKZ53_10445"
     CDS             300..1118
                     /locus_tag="PKZ53_10445"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNJ40898.1"
                     /translation="MKHEEIKELLELYTLGSLDSKDSRQVEGHLLECNECRQAVAVWR
                     DVMAELGFATEPVAPRSELRSQLLTAIQSEAQILPFRRPGGSPDISRQAIAPPQVGGQ
                     WLRWWALVASIGFISFAGGSLTLWQQLGGQQSQAHQIQQQLAQVQRELLQEREKAAWL
                     ASPQAVNARLLGTKESPAAQAKLSYDPQTGRTVLIVYNLPPPPPGKAYQLWFIADGVP
                     VSGGVFKIDQRGKAEMEGQVPNPGRAASLFAVTLEPEQGVPSPTGEKYLLSTTL"
     gene            1530..2735
                     /locus_tag="PKZ53_10450"
     CDS             1530..2735
                     /locus_tag="PKZ53_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HNJ40899.1"
                     /translation="MHRFSFRKFLFTSLTLLLIASTLLIPTPQINAADHGDAPLADED
                     RSCDINDVFAFLDPNDNTKLVIAVTIQGFIVPGEAVNFGAFDPNVRYRFALETSGDVK
                     ADQFFDLRFTERRTAGTEPQTATITLPGNRTFQAQTTPANLSPTASEPKITTDPTSGV
                     TFFGGVVDDPFTFDIPGFNRFFASAVAGNANPDLLKRGRDSFAGYNTLGIALSIPLSF
                     LKATGKDIGVAFFAQRQVKMTFKNGAYVGKGDFTTIDRMGNPAVNVALIPFPKKNSYN
                     FATPEDDARATFAADIINSLKTLGTTEANINILASVVLQRGDYIRVDPAKANSGPGGG
                     NNPEAAYPNGRRLGDDTVDTILFFIANQNVLSDNANKNDVPLRDAFPFFGASQQPRDP
                     GVVDDNTRN"
BASE COUNT          710 a          727 c          694 g          788 t
ORIGIN      
        1 cttcattggg atctccatag ttccctccac gcgcagcttc acggcaagcc ctgccgctgt
       61 ttgaccaaat cgaatttctt accgggtctc cggggagatt gtcgcgtgta agaaaagaaa
      121 gcgtattgtg atttggcgaa actcgcaagc aaaaaatcgg acggcattca tccgcgatca
      181 atcgcggggt ttcctgccga taatctataa aattgattgt caaccccaca aactcaaggc
      241 aagatcactc tttgcctgag tattttatca ccatttttct ttttggcatt ttttagggaa
      301 tgaagcacga agaaatcaaa gaattacttg aattatatac acttgggtca cttgacagca
      361 aagactcaag acaggttgaa gggcatctgc ttgaatgcaa tgaatgccgt caagccgtcg
      421 ctgtttggcg tgacgtgatg gctgagcttg gatttgccac tgagccggtt gccccgcggt
      481 cagaactccg ctcgcagctc ctgactgcta ttcaatctga ggctcaaatt ctaccatttc
      541 ggcgacctgg tggttcgcct gatatcagcc gacaggccat cgctccaccc caggttggag
      601 gtcagtggct gaggtggtgg gccctcgtcg cttcaatcgg gtttatctcg tttgcgggtg
      661 gcagcctgac attatggcaa caacttgggg gacagcagtc acaggcacac cagatccagc
      721 aacaactggc gcaggttcaa cgggagctct tgcaagaacg ggaaaaagcg gcctggctgg
      781 cgtctcctca agctgtaaat gcccgcttgc tgggcaccaa ggaatcacct gctgcccagg
      841 ccaagctcag ttatgacccg caaaccgggc gaacggtttt gattgtctat aatctgccac
      901 caccaccacc cggcaaagcc tatcagctct ggtttattgc cgatggggta cctgtctcgg
      961 gcggtgtttt taaaatagat cagcgtggaa aagctgaaat ggaaggccag gttccaaatc
     1021 cagggcgagc tgcgtcactc tttgctgtaa ccttggaacc agaacaaggg gttccttcgc
     1081 caactggtga aaaatatctc ttgagtacaa ctttgtaagt gatacattct tcgccagcag
     1141 tcacgttttc ttcgaccaaa cccgttccac cacattgagt catttactga agatacggat
     1201 ttttgaatga aaatcctgga ttttgacaat cttagctgct accagagagt tgtttggagc
     1261 aaactttcca ctccgtgaaa tccaaacaat tcttgtgggc cgtagcggac ttccgacttt
     1321 tcaccctttc aaatttcaaa aagctttttt gtattcctga tcaaggacct gtgtctgatc
     1381 cttattctga agacttgatc gcgagttttt gagtgaatgc gatgacactt ccttgagaaa
     1441 atcagtttga aactgtgaag cgagactcta ttttgacaac ctgaagtata gaattcacaa
     1501 ccggagacat aatccggagg aggaccccca tgcatcgttt ttcgtttcga aagtttttgt
     1561 ttacgagttt gaccctgctc cttattgctt caacactgtt gatccccaca ccccaaatta
     1621 atgctgccga tcacggtgat gcgccattgg ccgatgaaga tcgttcgtgc gatatcaatg
     1681 acgtttttgc ttttctggat ccaaatgaca ataccaaact ggtgattgcc gtgacgattc
     1741 aaggctttat cgttcctggt gaagctgtca atttcggggc ctttgacccc aacgttcggt
     1801 atcggtttgc attggaaacc agcggcgatg taaaggctga tcagtttttt gacctgcggt
     1861 ttactgaacg ccggactgcg ggtaccgaac cacaaaccgc caccatcacc ctgcctggga
     1921 atcgaacctt ccaggcacag acgacccctg ccaacctcag cccaactgcc tccgaaccaa
     1981 aaattacgac tgatccaacc tcgggcgtga cgttctttgg tggggtggtt gatgatccgt
     2041 ttacctttga catccctggt tttaaccgct tctttgcttc ggctgttgct ggaaatgcca
     2101 acccagattt gttgaagcgt ggtcgcgata gttttgccgg gtataatacc cttggcattg
     2161 ccctgagcat tccgttgtca tttctcaaag cgaccgggaa agatattggc gttgcctttt
     2221 ttgcccaacg tcaggtgaaa atgaccttta aaaacggcgc ctatgttggc aaaggtgatt
     2281 ttacaacgat tgaccggatg ggcaaccccg ccgtcaatgt ggcattgatc ccattcccca
     2341 agaaaaattc ctacaacttt gcgacgcctg aagatgatgc ccgcgcgacg tttgccgcag
     2401 atatcatcaa ctcattgaaa actttgggaa ccactgaagc caatatcaat attctggctt
     2461 ccgtggtgct ccagcgcggt gattatatcc gggttgaccc agccaaagcc aatagtggcc
     2521 caggtggtgg taacaatcca gaagcggctt atccaaatgg ccgccgcctt ggtgatgata
     2581 cggttgacac cattttgttc tttatcgcca accagaatgt cctgagcgat aatgccaaca
     2641 aaaatgatgt gccgttacgt gacgcgttcc cattctttgg ggcgtcccag cagccacgtg
     2701 atccaggtgt ggttgatgat aacacccgga actaattcct tttcgattgg ttaaacttga
     2761 gaaagaccac aaagaccagc gttgagcttt caattatgcg ctttgtggtc tttcttcttt
     2821 ccaaaatgaa cacaagtttt cattcggttt tttgtatgac gctctttgga accagattga
     2881 aatattgggc tggcgggatt ctggtagtag gagtattga
//
LOCUS       DAOQDO010000595         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27623_length_2915_cov_1.942657, whole genome shotgun sequence.
ACCESSION   DAOQDO010000595 DAOQDO010000000
VERSION     DAOQDO010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27623_length_2915_cov_1.942657"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2915)
                     /locus_tag="PKZ53_10455"
     CDS             complement(<1..>2915)
                     /locus_tag="PKZ53_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ40900.1"
                     /translation="IIPDAVTDSRFSENPKVAGPPHIRFYAGVPLTMPNGHRVGTLSI
                     IDDQPRQFSGEECERLQDLAVLVQDQIELRTNRLAPGYSEATLKKYNQVLVDLTSSQV
                     LSRGDLKASVQKLTCTTAQTLRVARVSLWMYADNDSKIRCIDLFELDTHRHSEGFELL
                     AKHYPTYFNSLEIERVIAAHDASSDPRTKEFSDSYLFPFGITSMLDAPVRLGNRKLGV
                     LCLEHIGPPRQWTLEEQQFAASLADLITVAIEAAEHQRAEIALRTSEQYLHHMLEHIG
                     LIAVQLDQQGTVTFCNRYLLEVTGYQKAQVIGKNWFEQFLPADQRVQAATRFRNNLAQ
                     QKITLHKEQEIETLTGNRRLISWYNTLLFGPAGDVEGTASIGIDITEKKQAEDTLRNS
                     EARNRILLDSIPDVMFRFSQLGNTLEYRPGKDLDPFGVTEDIIGTNVFEWMPDEVGQQ
                     VMYQMERALQTGEVQSLEFQTTIGALVRDYEARFVVSGQNEVLVIVRDIVKRKQIENE
                     LRQARNELELRVQERTAELEKANQALRHEISERKQFELALSESEERYRELFQNSPLGI
                     YRSTAEGQILMANPAMTRMLGYVSFDELKSCNLNNKSDFSPEFSRSQFLKMLLQTNEI
                     TGLETSWQRRDGTKIFVRENARAVFTPDGMLLHVEGTVEDITEHKLAELELIHSQERY
                     QILFESNPFPVFVFDAETLAFLAVNHAAVNHYGYSSEEFLAMTIKDIRPDEDVPKLLT
                     LLETIPPKTAIQGVWQHRKKDGTLIDVELTTHTMLFEGRPARSVIVNDVTDRKQAEEA
                     LRQSEERYRDLYENANDMFYTHDLQGRFTSCNKATERVSGYSRAEALTMSIAQVVAPE
                     DVEFAREMMLAKLSMNTPTTYELNFITKDGRCIPTEVSSRLIFKDGKPIGVQGTARDI
                     TERKQAEEMIKVARDSALESARLKSQFLANMSHEIRTPLNGVIGMTRLLADTKLTPK"
BASE COUNT          646 a          733 c          753 g          783 t
ORIGIN      
        1 tgtttggggg tcaatttggt atcggccagc aggcgtgtca ttccaatgac accattgagc
       61 ggcgttcgga tctcatggct catgttggcc agaaactgtg atttgagccg ggctgattcg
      121 agcgcgctgt cacgggcgac tttgatcatc tcttcggcct gtttgcgttc ggtaatatca
      181 cgcgctgtcc cctgaacccc gatgggtttg ccgtctttga aaatcagacg tgaactcact
      241 tcggttggaa tgcaccgacc atccttggta ataaagttga gttcataggt cgtcggggta
      301 ttcatcgaga gcttggcgag catcatttcc cgggcaaact cgacatcttc gggcgccacc
      361 acctgggcaa tgctcatcgt cagggcttcg gcccgggaat agccggaaac tcgctcggtc
      421 gctttgttgc acgaggtaaa tcgcccctgt aaatcgtggg tgtaaaacat gtcgttggca
      481 ttttcataca gatcccggta ccgctcttca ctctggcgca aggcttcttc agcctgtttg
      541 cggtcagtca cgtcgttgac gatgacgctc cgggctggcc ggccttcaaa caacatggtg
      601 tgagtggtca attcaacatc aatcaaggtg ccgtcttttt tgcgatgttg ccacacgccc
      661 tgaatggcgg ttttaggtgg aattgtttcc agcagagtga gcagtttggg gacatcttca
      721 tctgggcgaa tatctttaat tgtcatcgcc agaaattctt ccgacgagta cccataatga
      781 ttgaccgcgg cgtgattgac cgccagaaaa gccagggttt cagcatcgaa tacaaacacc
      841 ggaaatggat tgctctcaaa caaaatctga tagcgctcct ggctgtgaat gagttccagt
      901 tcggccagtt tgtgttcggt aatatcttcg actgtacctt caacgtgcag gagcatgcca
      961 tctggtgtaa aaacggccct cgcattttcc cgaacaaaaa tcttggttcc gtcacgccgt
     1021 tgccaggagg tttccaggcc ggttatctcg ttggtttgta acagcatttt cagaaactga
     1081 ctgcgtgaaa attcagggct gaagtcgctt ttattgttta agttgcagct ttttagctcg
     1141 tcaaacgata catatcccag catgcgagtc atcgctggat tggccatcag aatctggccc
     1201 tcggcggttg agcgataaat gccgagcggg gaattttgaa acagttcacg ataccgctct
     1261 tcactttcac ttagcgccag ttcaaattgt tttcgctctg aaatttcatg gcgcagggcc
     1321 tgattggctt tctccagttc ggcggtgcgc tcctgaaccc ggagttcgag ttcattgcgt
     1381 gcctgccgga gttcgttttc aatttgcttg cgtttgacaa tatcgcggac aatcaccagt
     1441 acttcattct ggccgctgac cacaaatcgg gcttcgtaat cacgaaccag ggccccaatg
     1501 gtggtttgaa actccagact ttgtacttct ccagtctgga gcgcgcgctc catctgatac
     1561 ataacctgct ggccaacttc atcaggcatc cattcaaaca cgtttgtccc aatgatgtcc
     1621 tccgtcacgc caaatggatc aaggtctttt ccaggtcggt attcgagcgt atttcccaat
     1681 tgactaaacc gaaacatgac atccgggatt gaatccagca gaatgcggtt tcgggcttca
     1741 ctgttgcgga gggtatcttc ggcctgtttc ttttcggtaa tatcaatgcc gatgctggcg
     1801 gtaccttcga catcgcccgc aggtccaaac aagagggtgt tgtaccatga aatcaaccga
     1861 cggttgccag tgagtgtctc aatttcctgt tctttgtgca gagtgatttt ttgctgggcc
     1921 aggttgttgc gaaaccgggt ggctgcctga acccgctggt cagccggtaa aaattgctca
     1981 aaccagtttt ttccaatgac ctgtgccttt tgatatccgg tcacttcgag caaatagcgg
     2041 ttgcaaaaag tcactgtgcc ttgctggtcc agttgcactg caatcagccc gatgtgttcc
     2101 agcatgtggt gcaaatattg ttcagatgtg cgcagggcaa tctctgcccg ttggtgttcg
     2161 gcagcttcaa tcgcgaccgt aatcaggtcg gccagcgacg ccgcaaattg ttgttcttca
     2221 agcgtccact ggcgcggtgg cccaatatgc tcaagacaga gaaccccaag ttttcgattg
     2281 cccagcctga cgggggcatc cagcattgaa gtaattccaa atggaaaaag atagctgtcg
     2341 gaaaattctt tggtgcgagg gtctgaactg gcatcgtggg cggcaatgac tcgttcaatt
     2401 tcaagtgaat tgaaataggt tggatagtgt ttggccagca attcaaagcc ttcactgtgt
     2461 cggtgggtat caagttcaaa caggtcaata cagcgaattt tcgaatcatt gtcggcatac
     2521 atccacaggc tcacgcgtgc cacacgaagc gtttgtgccg tggtacaggt gagcttttgg
     2581 acactggctt tgagatcgcc tctgctcaga acctgactgc tcgtcagatc aaccagcacc
     2641 tgattgtatt ttttaagggt cgcttcggaa tagcctggcg ccagacgatt ggttctgagt
     2701 tcaatttgat cttgaaccag aaccgccaga tcctggagcc gctcgcattc ctcgccagaa
     2761 aactggcgcg gctgatcatc aataatactg agcgtgccga ctcgatgtcc gttgggcatt
     2821 gtcagcggaa ccccggcata gaatcggata tgcggggggc cagccacttt tggattttca
     2881 gaaaaccgcg agtcggtaac cgcatcagga ataat
//
LOCUS       DAOQDO010000596         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27656_length_2913_cov_3.633660, whole genome shotgun sequence.
ACCESSION   DAOQDO010000596 DAOQDO010000000
VERSION     DAOQDO010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27656_length_2913_cov_3.633660"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKZ53_10460"
     CDS             complement(<1..366)
                     /locus_tag="PKZ53_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40901.1"
                     /translation="MTLLSLAAHLASAMADDQTWFRHPLIRRQGAPRITSFSPQSGPP
                     GTTVTIRGSNFVGVTAVRFNGFAAQSFQIDSASQITAVVSQNATTGLITVLSPLGTGT
                     SSDNFTVQSNNPTITSFTPT"
     gene            complement(556..1206)
                     /locus_tag="PKZ53_10465"
     CDS             complement(556..1206)
                     /locus_tag="PKZ53_10465"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ40902.1"
                     /translation="MSAVQSQLNPMPRINDDAPDFEAKTTQGMLRLSDLRGKWVVLFS
                     HPADFTPVCTTEFIEFARRTDDFVARNVQLIGLSIDSVFSHLAWVRNIEQNFNVQVKF
                     PVIADLDMKVASKFGLIHPNASETATVRAVFVIDEKGKVRAVIYYPMSCGRNMDEVLR
                     VIDALQTADKHGVATPANWCPGNDVIVPPPQTQEDAEKRLKSDYEITDWYFAKRKL"
     gene            1651..2778
                     /locus_tag="PKZ53_10470"
     CDS             1651..2778
                     /locus_tag="PKZ53_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40903.1"
                     /translation="MSTPSRKQTKSKFKLNTWHLIVPVIVIFLLGLWVLFGSANRARS
                     ELRILQDPDTIPPVQAQSATVPKPMAAPRAVAPLIPAQSNSAPTGDTSISAAPISTPQ
                     PIVASLPIAPAAPISGPATVPASCMPSAPVPPTPPVTVTALSSTEQELVRHSMVYAVL
                     PEDRQSNRNRGQLVYRGVPLRLSATISQPVIGTPGLVIQATLTQGIPGLSEAGALLTG
                     RVVQTVKNRVLVEFFRLTTRDGTQYRMTGTALDTTDGFNGLVGISKNSREWSKLLGQV
                     AGQALPFLKRGGTIVNNNGGSAQPAPTTPANVTSFLGGPVIVQIVTIDGIELPPPVNA
                     PSAPISPQPMPPPATPPTTLQPGGLGMMATSQSVPVPGVNQ"
BASE COUNT          768 a          743 c          708 g          694 t
ORIGIN      
        1 ggtcggggta aatgaggtaa tggttgggtt atttgattga acagtgaaat tgtctgaact
       61 ggtcccggtg ccaagtggcg acaggaccgt gatcaacccg gtcgttgcat tttgagagac
      121 gaccgctgta atttgactgg ccgagtcaat ttgaaaggat tgggcggcaa acccattgaa
      181 cctgaccgcg gtaacaccaa caaaatttga accacggatg gtcacggttg ttccaggagg
      241 acctgattgg ggagaaaaac tggtgattcg cggagcgcct tgacgccgaa tcagaggatg
      301 tctgaaccag gtctgatcat cagccattgc cgaagccaga tgggcagcca gagaaagaag
      361 cgtcaacacc aacccaagga aaacagtggg aaggggacga gaggaggcag gtaacgtgcg
      421 gaacataaac tgaagccaaa acttgataga ggaaaagtgg ctccccacct caactccagc
      481 atttcttaca tcaatctaaa gcgagatgga acagacaact ggtgtctgac cgacaccagt
      541 tgtcccagag ctaaattaga gtttccgctt ggcaaaatac cagtcggtga tttcatagtc
      601 ggatttcagc cgtttttcgg catcttcctg agtttggggc ggcggaacaa tcacgtcgtt
      661 tcctggacac cagttggctg gtgtggcaac accgtgttta tccgcggttt gcaacgcatc
      721 aatgacccgc aacacttcat ccatgtttcg tccacaggac atcgggtaat aaatcactgc
      781 ccgaactttg cctttttcgt caatgacaaa cacggcacgc acagtggcgg tttcactggc
      841 attggggtga atcaatccaa atttggatgc caccttcata tccaggtcag cgatgaccgg
      901 gaatttcacc tgaacattaa aattctgttc aatatttcga acccaggcca gatgtgaaaa
      961 cacactgtcg attgacagac caatcaattg cacattgcgg gcaacgaaat catccgtccg
     1021 ccgggcaaat tcaataaatt cggtcgtaca caccggtgtg aaatcggctg ggtgagaaaa
     1081 gaggacaacc catttgcccc gaaggtctga cagcctgagc attccctgag tcgtttttgc
     1141 ctcaaagtcg ggggcgtcgt cgttgattct tggcattgga ttgagttgag attgaacagc
     1201 actcatgatg aatttatctc cttgaaaagt aaggttccct aagtatctag aaggttcaat
     1261 ttttggtgcc ggaactggca cattgaactg gcgataaagc gacttgctga tttgggttga
     1321 gcacaatatc agtgaacgat acccaacgca atagaaccca atcgagcccc ttacctcatt
     1381 tcaagctccc atttctgccc tatttatgca ctctggtgtt cggtcaatgt cccggtttat
     1441 cgtccgagat cggggctggt tccatttttt tcccacctct cccaccttgc ttgtttctgg
     1501 tacaccgatt gctctgatta tcccatattg aaaaaattga attgaaatta aatttctatt
     1561 caccaaacag cgcttccaca ggtgaccaga tacgtttcca tctgtgaata gtctcgatca
     1621 ggccagactt gattttgaag gatgatgtgt atgagtacac catcaagaaa acaaactaaa
     1681 tcgaaattta aattaaatac ctggcacctt atcgtaccgg tgattgtgat ttttctactg
     1741 gggttgtggg ttttgtttgg atcagcgaat cgggcccggt cggaactgcg cattctgcag
     1801 gatccagaca ccatcccacc agtacaggcc cagtcagcca cggtgccaaa accaatggcg
     1861 gcgccacgtg cggtcgcgcc gttgattcca gcccaatcaa attcagcccc aacaggtgat
     1921 accagtattt cagcggcgcc gatttctacg ccacaaccaa ttgtggcatc gcttccgata
     1981 gcaccggctg cccccattag tggccccgcc acggtaccag cttcatgcat gccatcggca
     2041 ccggtaccac cgacaccacc agttacagtc accgctctgt cttccactga acaggaactg
     2101 gtacgtcatt caatggtcta tgctgtgctc ccagaagatc gccaatcaaa tcgcaaccgg
     2161 ggacagttgg tctatcgagg tgttccgctc cggctctcgg ctaccatcag ccagccagtg
     2221 attggcacac cgggactggt cattcaggca acattaaccc aggggattcc gggtctctct
     2281 gaggctggtg ctcttctgac gggtcgggtg gtccaaactg taaaaaaccg ggttttggtt
     2341 gagttcttcc gactcacaac ccgtgatggg acacagtatc gaatgactgg aactgccctc
     2401 gatacaaccg atgggttcaa tggactcgtg ggcatttcaa aaaattccag ggaatggtcc
     2461 aaattgttag gtcaagtcgc aggccaggca ctgccctttc tgaaacgagg tggaaccatt
     2521 gtcaataata atggaggcag tgcccagcca gcacctacca caccggccaa tgtcaccagc
     2581 ttcttgggag ggccagttat tgtccagatt gtcacgattg acggaattga attgccacct
     2641 ccagtcaatg cgccatcggc accaatcagt cctcagccaa tgcctccgcc agcgacaccg
     2701 ccaaccacat tacaaccagg gggactgggg atgatggcca ccagccaatc ggttccagtt
     2761 ccaggggtaa accaataatg ccattttgga gtgatccggt tggagaacag tcaaataatt
     2821 caattaaatg gagttatgga acaactaacc aggtactact gacgactgtc cactggctaa
     2881 aaactggtat aaacatatga caaacagtaa act
//
LOCUS       DAOQDO010000597         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27675_length_2912_cov_4.026951, whole genome shotgun sequence.
ACCESSION   DAOQDO010000597 DAOQDO010000000
VERSION     DAOQDO010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27675_length_2912_cov_4.026951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..558)
                     /locus_tag="PKZ53_10475"
     CDS             complement(61..558)
                     /locus_tag="PKZ53_10475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ40904.1"
                     /translation="MEKPKGLKFFVADMMEADLEEAVELEETTGLSRWGYEGYHWDLH
                     YNPMAIMLVARGDEPGAMMRRILGFIVGRVVPSPVEDYDELHINNIATHPDFRRFGVG
                     WALMEEAMSRGRIYGAKSVRLEVRASNTAAQLLYYQLGFQAVTRRRNYYYGPTEDAVV
                     MERWL"
     gene            complement(569..1390)
                     /gene="tsaB"
                     /locus_tag="PKZ53_10480"
     CDS             complement(569..1390)
                     /gene="tsaB"
                     /locus_tag="PKZ53_10480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNJ40905.1"
                     /translation="MTVSSPPDSPVLLLLDTTSTQFSVALVRGSQILAEFGVVTRQTS
                     SLVISDIDFVLQRAGCALAGLDGFGVLVGPGSFTGLRVGLATIQAMSQALNRPVVTAT
                     TLEVLTATIPVFLNPVWVWVVHTSYRDEVYAQCFRLEPGTGPQPITEPFAGHKHQIAD
                     IITVFLRNPPTFAPAGFFTGDAIPVLSARLQAEADEQQVQFFPLDQTSQMNYLIESGG
                     WYCIQSPTFLAPTAAGLFLNRWREGKQIGAHEIDACYVRPSEAELKLIQPAESQA"
     gene            1742..2758
                     /locus_tag="PKZ53_10485"
     CDS             1742..2758
                     /locus_tag="PKZ53_10485"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNJ40906.1"
                     /translation="MTGGGDAPGLNAALRAVVRRAMQAHLRVLGVHHGWKGLIQGKVE
                     PLTRYSISGILPRGGTILGTSRINPLESDDTLHRIQENWKRFGLDALITIGGDGTLSA
                     ALRMWRDQGYPIVGIPKTIDNDVRGTDFTLGFDTAVSIATEAIDRLHSTAESHDRVMV
                     VEVMGRHAGWIAATSAIAGGADMVLVPEYPFRISRICEIINQRKSLGRFFSIIVVAED
                     AHPHPDENFLSEQDAQEVFEHTRLGGIGSLLGKKIEELTGIVTRVTVLGFVQRGGGPT
                     AFDRMLASRMGVKAVDMVLNHEFGSMTAIQGTRMVSVPLEHAVSGIKLLDDSIYRDCE
                     VFFG"
BASE COUNT          698 a          778 c          745 g          691 t
ORIGIN      
        1 ttcattcttc attcttcatt ctcaattctt cattcttcat tctcaattct tcatttagcc
       61 ttacagccag cgttccatca cgacggcgtc ttctgttggt ccataataat aattgcggcg
      121 gcgggtgaca gcctgaaaac caagctggta atagagcaac tgtgcggctg tgtttgaagc
      181 ccgaacttcc agccggacac ttttggcgcc atagatgcga cctcgtgaca tcgcctcctc
      241 catcagtgcc cacccaacac caaatcgcct gaagtctgga tgtgtcgcga tgttgttaat
      301 atggagttcg tcataatctt cgaccggaga gggtacgact cgaccgacaa taaagccaag
      361 aatgcgacgc atcatggctc ctggttcatc accacgggcg accagcatga tggccatcgg
      421 gttgtaatgc agatcccaat gatagccttc atacccccaa cgactcagcc cggtggtttc
      481 ttcaagctcg accgcttctt ccaggtcagc ttccatcata tccgccacaa aaaacttgag
      541 tccctttggt ttttccattt tcgacacctc acgcctggct ttcggctggc tggatcaatt
      601 tgagttcggc ttcagatgga cgaacatagc aggcgtcgat ttcgtgggct ccaatctgtt
      661 ttccctcccg ccagcgattg aggaaaagcc ccgctgccgt tggtgccaga aaggttggag
      721 attgaataca ataccaaccg cctgattcaa tcaggtaatt catttgcgaa gtctggtcaa
      781 gtggaaaaaa ttggacctgc tgctcgtcag cttcagcttg tagacgggca gacaggacag
      841 gaatcgcatc tccggtaaaa aaccctgcag gagcaaacgt cggagggttt cggagaaaca
      901 cagttatgat gtcggcaatc tgatgtttgt gaccagcaaa aggctcagtg attggctgcg
      961 gtcctgtccc cggctcaagt cgaaaacact gcgcatacac ttcgtcacga tacgaagtat
     1021 gcacgaccca gacccaaacc gggtttaaaa agactggaat cgtggccgta agcacttcga
     1081 gcgtcgtggc cgtaaccact ggccgattca gggcctggct catcgcctgg atggtcgcca
     1141 gtccaacgcg aagaccagta aagcttcccg gaccaaccag cacaccaaac ccatccagcc
     1201 cggcgagcgc acagccagca cgctgcagga caaaatcaat gtcactgata accagagatg
     1261 aggtttgtct ggtaacgacg ccaaattcgg ccagaatctg gcttccccga accagcgcaa
     1321 cgctaaattg ggtggaagtt gtatccagaa gcagaagtac aggggagtca ggaggtgagg
     1381 aaacagtcat gagaatttgt ggtgaggtgt aataccattt agggttcagg gttcagggtt
     1441 caggaaaaga caaaaaggac ggaaaggacg aaagttcgaa acccctgaac cctgaaccct
     1501 gaaccctgtc agtttgggta aaatggtgat gtttgacaca tcttgtcgct caactatata
     1561 gttgcacgac ttgctctgac aatcctgcgg ctcttcaacc ttttactcct caccttcttt
     1621 ttgaagtgaa acccaggtta gcgaccttct tccagagcag gtgattgtac aatcagtgag
     1681 ccatcagcag tactcttttt cagaggacct tcacgtgaaa gacattcgaa ctattggtgt
     1741 tatgacaggc ggtggcgatg caccggggct gaatgcagcg ttacgcgccg ttgttcgccg
     1801 ggcgatgcaa gctcacctcc gggtgctcgg ggtccatcac ggctggaaag gactgattca
     1861 aggcaaagtt gagccgctga cacgatattc aatttcaggg attcttcctc ggggcggcac
     1921 aatccttggt acctcgcgca tcaacccact ggaatcagac gataccctgc atcgtattca
     1981 ggaaaactgg aagcggtttg ggcttgatgc cctgattaca attgggggtg atggcacact
     2041 gagtgccgcc ctgcgcatgt ggcgcgatca agggtaccca atcgtcggaa ttccaaaaac
     2101 gattgataac gatgtgcgcg ggactgactt tactcttggt tttgataccg ctgtgtcaat
     2161 cgccaccgaa gccattgacc ggttacacag cacggctgaa tcccacgacc gggtcatggt
     2221 ggtcgaagtg atgggccgtc acgccggctg gattgccgcc acttcagcca ttgccggagg
     2281 tgccgacatg gtgctggtac cggaatatcc gttccggatt tcccgaattt gcgaaatcat
     2341 caaccagcgc aaatcgctcg ggcgcttttt ttcaattatc gtagtggccg aggatgctca
     2401 cccgcatccg gatgaaaact ttctttctga acaagatgcc caggaggtat ttgaacatac
     2461 ccgactgggc gggattggct cactgcttgg aaaaaaaatc gaggaattga ccgggattgt
     2521 tacccgcgtc acagtgctgg gatttgtcca gcgcgggggt gggccaacgg cgtttgaccg
     2581 aatgctggcc tcacggatgg gggtcaaagc cgtggatatg gtgctcaacc atgaatttgg
     2641 ctcgatgacg gccatccagg gaacgcgtat ggtctcagtt ccactcgaac acgccgtctc
     2701 tggtattaag ctcctggatg acagtattta ccgcgattgt gaagtctttt tcgggtagcc
     2761 cgctgctcac cacctggctg cctgaccttc cttccctgct gacaacaagc agccacaatc
     2821 catcgcttgt cttaaactca aaacaactcc tcaaatcctt ttttttcaga gagatcccct
     2881 gggttcccca tccaggcggc gccatgtctt gt
//
LOCUS       DAOQDO010000598         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27680_length_2912_cov_2.934547, whole genome shotgun sequence.
ACCESSION   DAOQDO010000598 DAOQDO010000000
VERSION     DAOQDO010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27680_length_2912_cov_2.934547"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKZ53_10490"
     CDS             <1..642
                     /locus_tag="PKZ53_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40907.1"
                     /translation="KKIGNGDDPPNNLYWGCEFGVKTHLKRAQEWKVVSEIQSPKPGV
                     LERVIFQHRTKPVFLVADAYQGREIKQCTLDLLRFAAGGGTETIAIPGASAPVYAGGS
                     SHLLAYVGHDGLMDFQLDAIPQRQDQSKREVIILACASQQYFSDPLKQTGAQPLLWTT
                     GLMAPEAYVLKAGIDGWIATESGEAIRSRAAKAYTTYQNCGMKGAMRLFATGW"
     gene            complement(789..1910)
                     /locus_tag="PKZ53_10495"
     CDS             complement(789..1910)
                     /locus_tag="PKZ53_10495"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNJ40908.1"
                     /translation="MNSATKKQGIGLIMFVVLLSAGVFWLVMPQLTLAQKPQPRKLFP
                     ISKKGKCGFIDVTGKIRIAPVFDNVENFSEGLAAAEVNRKWGYIDEAGKWVIKPKFDM
                     ALQFSEGLAQVRIGGRWSAEPVYLTGGQAAYIDRSGKIVFTLAQAPLGTAYNEYPFSE
                     GRLPFEHGPKYGYLDQTGRTVIAPTFAYAGDFSEGLAGIVLNEKFGFIDKNGKVVIAL
                     NYLPNHWGNPEGFSEGLVSVRMENGKLGYLDKTGAVAIAPQFYWAESFSEGLAVIQFT
                     QDGKCGYIDKSGKLVISPEFELAESFSEGLAAVRIDCNLWGYINRTGKLVIEPQFRSV
                     KRFSGGLAEITIGERWGYINQSGKVVWMSGPPTSQPKHQ"
     gene            complement(1931..2266)
                     /locus_tag="PKZ53_10500"
     CDS             complement(1931..2266)
                     /locus_tag="PKZ53_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40909.1"
                     /translation="MGRYNSFYFDSECTFCGTRELRDFQAEIGVLVWDNYELGDEIRW
                     ELLASRPIPIGPNGERPDGHYWATGIAHCRHCREDLIGRVLIRNNQYVGVEIPDVPDG
                     LYDWGTLPE"
     gene            complement(2493..2621)
                     /locus_tag="PKZ53_10505"
     CDS             complement(2493..2621)
                     /locus_tag="PKZ53_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40910.1"
                     /translation="MESSRWSRSFGRYHRITAQFHLAPGWALEDFVGQVSSILQTR"
BASE COUNT          732 a          773 c          668 g          739 t
ORIGIN      
        1 aagaaaattg gaaatggcga cgatcctcca aacaatttgt actggggctg tgaatttggg
       61 gtcaaaaccc atttgaagag agcccaggaa tggaaagtgg tttccgaaat tcagtcaccg
      121 aaacctggcg tgcttgaacg ggtgattttt cagcatcgaa ccaaaccagt gttcctggtt
      181 gcggatgcct atcaaggccg tgaaatcaaa cagtgtacac ttgatctttt gcgctttgcc
      241 gcaggaggtg ggacggagac gattgccatt ccaggtgcat cggcaccagt ttatgctgga
      301 ggctcatccc atttactggc gtatgttggc cacgatggac tgatggattt tcaacttgat
      361 gccattcctc agcgccagga ccagtcaaaa cgggaggtga tcattctggc ctgtgccagt
      421 cagcagtatt tttcagatcc cttgaaacaa accggcgccc agccacttct atggaccact
      481 ggactgatgg cccctgaagc ctatgtcttg aaagccggga ttgacggatg gattgcaact
      541 gaatcaggtg aggccattcg aagccgggcg gcaaaagcct ataccaccta tcagaactgc
      601 gggatgaaag gcgcgatgcg gttatttgca actggctggt gaggttcagg acctgggagc
      661 atcagcacca ggataccagc caatgtgtca tctgaaaaag aaacacgata gcaattactc
      721 tcaatatccc tctcactgga atcactggtt aggtctgttt cgaatcaggc atcagaaaaa
      781 ctccttgctt actgatgttt tggctgactg gttggcggac ctgacatcca gacgactttt
      841 ccgctctggt tgatatatcc ccatctctct ccgattgtga tctccgccag cccgcctgaa
      901 aatcgcttta cacttctaaa ttgtggctca attacgagtt tgccggttcg gttgatatat
      961 ccccataggt tacaatctat tcgcacggca gccagtcctt ccgaaaaact ttctgccaac
     1021 tcaaactctg gtgaaattac cagtttccct gacttatcaa tatatccaca cttcccatcc
     1081 tgggtgaact gaatcaccgc cagcccctcg gaaaaacttt ctgcccagta aaactgtgga
     1141 gcgatggcaa ccgccccggt tttatccagg tagcccaatt ttccattttc cattcttact
     1201 gaaaccaatc cttctgagaa gccttctggg ttgccccaat gattgggtaa gtagtttaga
     1261 gcaatcacca ctttcccatt tttatcaatg aatccaaact tctcattcag cacaataccc
     1321 gccagccctt ccgaaaaatc accagcgtat gcaaatgtgg gagcaatcac cgtccgtccg
     1381 gtctgatcca gatagccata ttttggccca tgttcaaacg gcaaccggcc ttcagaaaaa
     1441 ggatactcat tgtaggccgt ccccaacggg gcttgtgcca gggtaaaaac aattttgccg
     1501 gagcgatcaa tatacgctgc ctgccccccg gtcaaataga ccggttcggc tgaccatcgg
     1561 cctccaatcc tgacctgagc cagtccttcc gaaaactgaa gtgccatgtc gaattttggt
     1621 ttaatcaccc acttcccagc ctcatcaatg tagccccatt ttcgatttac ttcagccgct
     1681 gccagtcctt cagagaagtt ttcgacgttg tcaaaaactg gagcaatccg aattttcccg
     1741 gtcacatcaa tgaacccaca tttccctttt ttactgatgg gaaataactt ccggggttga
     1801 ggtttctgag ccaaggtgag ttgtggcatc accaaccaga acacaccagc gctcaacaac
     1861 accacaaaca ttatcagccc gattccctgc ttttttgttg ctgagttcat ttcgtatctc
     1921 cattcttctt tcattcagga agcgtccccc agtcatacag cccatctgga acatctggaa
     1981 tttcaactcc gacatactgg ttgtttcgga tgagcacccg gccaatcaga tcttctcggc
     2041 aatgcctgca atgagcaatc ccggttgccc agtagtgtcc atctggccgc tccccgtttg
     2101 gtccaattgg tatgggtcgt gatgccagta gttcccaccg aatttcatca cctaactcat
     2161 aattatccca taccaacaca ccgatctcag cctggaaatc acgtaattct cgtgtgccac
     2221 aaaatgtgca ttcggaatca aagtaaaacg aattgtagcg ccccatcctg gcctccgttc
     2281 aaggaatatc tgtttataaa tcaaatattt cgctgaggtt ccgcctgctc aatcaaggtt
     2341 ctgaagtact caaaatctgt ttctgtttcg gaaaaattgg gtttcgcccg gatgggcgcc
     2401 ggatagtagc cggagggaag cctgctttga ggcgcgccct ccggaatgcg ggctgaggca
     2461 attcgcgccc ggatgggcgc tggatcaaaa tctcaacggg tttgcagaat tgaggatacc
     2521 tgaccgacaa aatcttccag cgcccatccg ggcgcgagat gaaactgggc cgtgatccgg
     2581 tggtagcgcc caaagctgcg cgaccaccgg ctactctcca tcgtcctccg gacgggaaag
     2641 gtcaagatta aacagtctct ccacaactgg gcgcttttat aaaatagagc ttgaaatgag
     2701 tcgccgattg ccggtaagac ttgagtctca gcatatttac aacaggagta tcgcctatgg
     2761 gagtccgcaa tatcattttg tcaattgttc caaaatcctg ggctatttca atggaagccg
     2821 agtctcgtgc ctggatgatg aaatgtgaag gttgcaacca cgaaatatca gtctgggatg
     2881 cgggcggtct ccgttggaaa gcgaagggaa at
//
LOCUS       DAOQDO010000599         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27685_length_2912_cov_2.647182, whole genome shotgun sequence.
ACCESSION   DAOQDO010000599 DAOQDO010000000
VERSION     DAOQDO010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27685_length_2912_cov_2.647182"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2912)
                     /locus_tag="PKZ53_10510"
     CDS             complement(<1..>2912)
                     /locus_tag="PKZ53_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNJ40911.1"
                     /translation="TTGTGAGVWNSTAASFGTGVENVNTSSAPGRDVIYQFTAPSAGD
                     YSFSVTNGQVLSTGLNLVTYLVNGPTCPTAGLVTILAGANRTTAHEQIACVPLTSGQT
                     VFLIVDDNTGANEDGFFVQVTQCSKEPEFAPSALNGTNDSTGTATAFVNGITGQIGAA
                     GDVDFYPIPNQAVGSRVFAIVNGAFSASTNFDMRITNATDTLEFDNLDNVTMFGGSAP
                     NIAGRPLTAASGQFFRVNQNSASTASAPYQLFAVTRPMGTEVAETAAANDTIDDAGIQ
                     TGNYFSGSFSSTADVDLYKFPVTAGDLIFVSFDGSPDRTGTLATDALLKLLDPAGNAL
                     VQTDDFTNSSSTTSGAGSLTATTPNSPAEALLFQARTSGTYYVRVNPFTGTTYLLSIA
                     TISNADVSITKTDGVTSVNQGGTLTYTITATNNGPGNATGTIITDNFPAQITTANWTA
                     SYGGGASGPASGTGNINATIGAFPSGGTATFTVTATVGSTLGLITNTATATTPVGVTD
                     PTPGNNSATDNNTNVIATPCILTCPSNIVTNNSAGQCGAVVTFGNPTTTGSCGTVTAV
                     PPSGSFFSVGSTMVTFTSTAGPSCSFTVTVNDTEPPVVTCPTNITVLASSSAGSVVTF
                     TPTASDNCPGVVVTSDPASGSTFPVGTTIVTVTATDASNNTATCTFDVNVEGVERAGI
                     YVADTQNNRCQMSNGFVWGVVPGAPGTVGSGNGQFRLPEAIVANFPGQRIYVADTGNN
                     RIQYTTNGGGTWQNFATLGSGANQVRAPQGLAIDNLGNLYVSDTGNNRVLRFDGGVPG
                     NAVILAAAGSTAGKVNSPRGLAIDLDFNLYVAEFQNNRIQRFANANTASPTASLVATV
                     GSSLSPGQVRAPEGVAVDNAGNLYVADTGNNRIIMFTANAPSTLSTAATLICSSGSAL
                     GQVRAPEGVVIGAGEILGGGPTSVAIFISDTGNNRVQGSFLPTGPGSFVLVSTPNN"
BASE COUNT          694 a          755 c          791 g          672 t
ORIGIN      
        1 cattgtttgg tgtactgaca agcacaaacg aacctgggcc ggttggcaag aaactgcctt
       61 ggacccgatt gttcccagtg tcgctgataa agatggcgac cgaggttggt cccccaccaa
      121 ggatttctcc cgcaccaatg acaacgcctt caggcgcccg gacctgaccc agcgccgagc
      181 cggagctgca aatgagcgtt gccgcggtcg aaagggttga tggcgcattt gcggtaaaca
      241 tgatgatccg attgttgcca gtatcggcca catagaggtt tccggcatta tcaaccgcca
      301 ccccttcagg tgcccgaact tgaccagggg acagcgatga gccaactgtc gcaaccagac
      361 ttgccgtggg tgaggctgta ttggcattgg caaaccgttg aattcgatta ttttggaact
      421 cggccacgta caaattgaaa tccaggtcaa ttgccagccc acgcggactg ttgactttac
      481 cagccgtcga accagccgcg gccaggatca cggcattgcc tggaacaccg ccatcaaagc
      541 gtaagacccg attgtttccg gtatctgaca catacaaatt ccctaaatta tcaattgcca
      601 gaccttgtgg cgcccgaacc tggttggctc cggatccaag tgtggcaaag ttttgccagg
      661 tgccaccgcc attggtcgtg tactgaatcc ggttattccc agtatcagcg acataaattc
      721 gttggccagg gaagtttgcc acaatggcct ccggaagccg gaactgtcca ttcccagagc
      781 caactgtacc cggcgctcca ggaacgactc cccacacgaa cccatttgac atctgacacc
      841 gattattttg agtgtcagct acataaatcc cggcacgttc cacgccctct acgttgacgt
      901 caaaggtgca ggttgccgtg ttgtttgaag catcagtcgc ggtgacagtg acgatggttg
      961 ttccgacggg gaaggttgat ccagaagccg ggtcagatgt caccacgaca cccggacagt
     1021 tatccgaggc ggttggcgtg aaagtcacta ccgacccagc acttgaagaa gccaaaacgg
     1081 taatattcgt cggacacgtc accactggtg gttcggtatc attgactgtc accgtaaatg
     1141 agcagcttgg ccctgccgtt gaagtaaagg tcaccatggt tgatccaaca ctaaagaacg
     1201 agcctgacgg tggcactgcg gttacggttc cacacgatcc ggttgtggtc ggattgccaa
     1261 aagtcaccac ggcgccgcat tgtccagcac tattgttggt cacaatgttg gatggacacg
     1321 tcaaaataca tggcgttgcg atcacgttgg tgttgttgtc agtggcgctg ttatttcctg
     1381 gggtcgggtc tgttacacca accggggtgg tggcagtggc agtatttgta atcagaccca
     1441 gggtactgcc aaccgtagct gttacggtaa aggtcgcagt acctcctgac gggaaagcgc
     1501 cgattgtggc gttgatgttt ccagttccgc tggccggccc actggcacca ccgccatagg
     1561 aggcagtcca gttagctgtt gtaatttgag ccgggaaatt atcggttatg atggtacccg
     1621 ttgcattgcc aggtccatta ttcgtcgcag tgatggtata ggtcagagtg ccaccctggt
     1681 tgacagatgt tacaccatcc gttttggtga tgctcacatc agcgtttgag atcgtcgcaa
     1741 ttgacaacag gtaggttgtc ccagtaaaag gattgactcg aacatagtag gtcccggaag
     1801 ttcgagcctg gaaaagtaag gcttcagccg gtgaatttgg ggttgtggcc gtcaagcttc
     1861 cggctcccga ggtggtactg gatgaattgg taaaatcatc ggtttgaacc aacgcatttc
     1921 cggctggatc caaaagcttc aacagagcat ctgtagccaa tgtcccagtg cggtctggtg
     1981 aaccatcaaa gctgacaaaa atcaggtctc cagccgttac cgggaattta tacaggtcaa
     2041 catcggcggt cgaactgaaa cttcctgaaa aatagtttcc agtctggatt cccgcatcat
     2101 caatcgtgtc attggcagcc gcagtctcag ccacttcagt tcccattggt cgggtaacgg
     2161 caaagagctg gtagggagcc gaggctgttg aggctgagtt ttgattcacc cggaaaaact
     2221 gacccgaagc tgccgtcaat gggcgacctg caatatttgg ggcggaacca ccaaacatcg
     2281 tgacgttgtc gagattgtca aattccaggg tatcggtggc attggtgatc cgcatatcga
     2341 aattggtgct ggctgaaaaa gcgccattga cgatggcaaa aacccgcgac ccaacagcct
     2401 ggtttggaat tgggtaaaaa tcaacgtcgc cggcagcccc aatctggccg gtgatgccat
     2461 tcacaaaagc cgtggcagtt ccggtcgaat cattggtccc gttcagggca ctcggtgcaa
     2521 attcaggttc ttttgaacat tgggttacct ggacaaagaa gccgtcttca ttggccccag
     2581 tgttgtcatc cacaatcaga aataccgttt gcccggaagt taacggaaca caggcgatct
     2641 gttcgtgggc tgtcgttcgg ttcgcaccgg ccagaatcgt taccagaccg gcagtcgggc
     2701 aggtgggtcc attcacgagg taggttacaa ggttgagacc agtcgaaaga acctgcccgt
     2761 tggtgacact aaatgaataa tcccctgccg aaggcgccgt aaattgataa atgacgtctc
     2821 ttccaggagc tgaggaagta ttgacgtttt ccactccggt tccaaaactg gcagccgtgg
     2881 aattccagac accagcacca gttccagtag tg
//
LOCUS       DAOQDO010000600         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27739_length_2908_cov_3.710130, whole genome shotgun sequence.
ACCESSION   DAOQDO010000600 DAOQDO010000000
VERSION     DAOQDO010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27739_length_2908_cov_3.710130"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..670)
                     /locus_tag="PKZ53_10515"
     CDS             complement(245..670)
                     /locus_tag="PKZ53_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40912.1"
                     /translation="MKSRLLFAATIIFSLGLTLNPVYGGLPKSAAFTPTMTIDNPALD
                     FTIVNKTGYGIKALSIGATGTGDWAPEDEVLHGRAFANGSALEVKFHPRATAAKWDIL
                     VVWADGSGGAEWEGLDLTTINKLTLVYDADSDTTSAEAE"
     gene            complement(1016..1441)
                     /locus_tag="PKZ53_10520"
     CDS             complement(1016..1441)
                     /locus_tag="PKZ53_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40913.1"
                     /translation="MKSRSLFAATIILSLGLTLNPVYGAPTGPAAFTPTATIENPALD
                     FTLVNRTGYSIKAVYIGASGTGDWTRDDEVLKGRTFRNGAALDITFHPRATAEEWDIM
                     VQWSDGSGSEEWLNLDLTEISKVTLVYDGEKDVTSAIIE"
     gene            complement(1761..2186)
                     /locus_tag="PKZ53_10525"
     CDS             complement(1761..2186)
                     /locus_tag="PKZ53_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40914.1"
                     /translation="MRFLPLFAASVVLFFGLTVPPCHAALPDSPSPSALAVTQNANLD
                     FTLVNRTGYSIKAVYIGASGTGDWTRDDEVLKGRTFRNGAALDITFHPRATAEEWDIM
                     VQWSDGSGSEEWLNLDLTEISKVTLVYDGEKDVTSAILE"
     gene            2609..>2908
                     /locus_tag="PKZ53_10530"
     CDS             2609..>2908
                     /locus_tag="PKZ53_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40915.1"
                     /translation="MGIVRWVNRIEPARYEQVCAILTGKDVRNIDDVKQILTVLETPV
                     DEEYLEQLEYWFDEDSSESQSHILFSILERAIIEESWELDKALDSGLDTVFRVLPG"
BASE COUNT          786 a          626 c          760 g          736 t
ORIGIN      
        1 taagtacctt accgaaaatt tccagacatt ttgaaccacg gaaaacacgg aaaacacgga
       61 aaaaatcaag cacttaccaa acccaatatc tcaggaaact tatgataaag tacttatgta
      121 tgtagccaaa acaaaaaagg ccgccaggaa gataagtcgt cgttactttc tcgctggcgg
      181 ccatcattgt aagagaaact ggataaaacg atcactcaaa ggtcaaaaac aaaagggact
      241 ggtgttattc ggcttcggca ctggttgtgt cagagtcggc atcatacacc agggtaagtt
      301 tgttgatggt ggtcaggtcc agaccttccc attcggcccc gccggagcca tcggcccaaa
      361 caacgagaat atcccacttg gccgctgttg cgcgggggtg gaatttgact tccagagctg
      421 agccgttggc aaaggctcgc ccgtggagca cttcgtcttc cggcgcccaa tctcccgtac
      481 ccgtggcccc aatcgagagg gctttaatgc catagccggt tttgttcaca atcgtgaaat
      541 caagggccgg gttatctatg gtcattgtgg gagtaaatgc tgctgacttg gggagtccac
      601 cataaactgg attaagcgtt aatccaagac taaagataat ggttgctgca aacaataatc
      661 gtgacttcat gaggttctcc taaaggtaaa atacacaaac accagggaat ttgccctcca
      721 ctataatttt ggaggtgcag acacaaagag ggtcaagcct gtaggctgga tttggtgtgg
      781 tcttagagag aagtcacgaa acgaacggtg tgtgttggtc ctgaaaacac tggcttcctg
      841 aagtgatgaa ggacaggaag gtttgattgc gacttctctt ttaccattgg gttaattttg
      901 ggtcaagctt tatattttcc atcgaaaagc tgaccacaat gaaacgcagg atggaggtcc
      961 gctcaacaaa gatgctgtcg tcggactcgt ggttaatgac cgtaacggcc agggtttatt
     1021 cgataatggc gctggtgaca tctttttccc cgtcatagac cagcgtaact ttggaaattt
     1081 cagtcaggtc cagatttaac cactcttcgc tgcccgaacc atcggaccat tggaccatga
     1141 tgtcccattc ttcagccgtg gctcgcggat ggaatgtaat atccagggcc gcaccattgc
     1201 gaaaggttct tcctttgagc acttcatcat ctctggtcca gtcaccggtt ccgctggcgc
     1261 caatgtagac ggctttaatg ctgtatccgg ttcggttaac gagtgtgaaa tcaagggccg
     1321 ggttttcgat ggtggccgtg ggagtaaatg cggctgggcc ggtcggtgca ccgtaaacag
     1381 ggttcagggt taacccaaga ctgaggataa tcgttgcagc aaacaaagat cgtgacttca
     1441 tgaggttctc ctaacggtaa actgcacaaa caccagagga tttgccctcc actataattt
     1501 tggaggtgca aacacaaaaa gagtcaggcc tgtagagggc tagagttggt atggttgtgg
     1561 agagaagtca cgaaacgaac gaggtgtttt ggtcctgaaa acactggctt cctgaagtga
     1621 tgaagaacag gaaggtttga tgacaaaacc ccttttacca tcgagtctgt tttgggtcaa
     1681 gccgtttgtt ttgaggccac aatcgccttc acaaacagcc gagccagtat gtttgagggt
     1741 gctttttcga cagatagccg ttattcaaga atggcgctgg tgacatcttt ttccccgtca
     1801 tagaccagcg taactttgga aatttcagtc aggtccagat ttaaccactc ttcgctgccc
     1861 gaaccatcgg accattggac catgatgtcc cattcttcag ccgtggctcg cggatggaat
     1921 gtaatatcca gggccgcacc attgcgaaag gttcttcctt tgagcacttc atcatctctg
     1981 gtccagtcac cggttccgct ggcgccaatg tagacggctt taatgctgta tccggttcgg
     2041 ttgacaagag tgaagtcaag gttggcattt tgggtaacgg cgagtgccga tggcgagggg
     2101 ctgtcaggca aagcagcgtg acacggaggt actgttaagc cgaaaaatag aacaactgat
     2161 gccgcaaaca gaggcaggaa tctcatggaa atctccttag aacatggaag gaaccaggca
     2221 caactgtacc ggacctgaaa gggaatgtgt gaatgcggcg tgaacgagac aggtaaaacc
     2281 aggagacgta ttgagaagtg atagacaata aataaacttt gacgtgtgga cttcatatag
     2341 catcaggtca gtccgtgggt caaggttcaa tctgtttttg ttatgcctgc caggattcaa
     2401 ccagtcctta cgtggttgcc tgcaggaaaa ttaatttttt attgtcaaac gagaggctta
     2461 tttctaagat ggggtttggc ggaatactat caggtaaacc tttgccattt gcataggaaa
     2521 gtccgcaaga taatgtgttt tctatcagtt atgccggata ggaaaagctc tgtatttgat
     2581 ggttaatcgg tttttcaaag gaggaaaaat ggggattgtc cgatgggtga atcgaattga
     2641 accagcccga tatgaacagg tttgtgcgat tcttacgggg aaagacgttc gaaacattga
     2701 cgatgttaaa cagattctca ccgtacttga gacacccgtt gatgaagaat atctggagca
     2761 actggaatac tggtttgatg aagattcgtc cgaaagccag agccatatcc tcttttcaat
     2821 tctggaacgg gcgattattg aggaaagctg ggaactggac aaagcactgg atagtggact
     2881 ggatactgtt ttccgggtgt tgccaggg
//
LOCUS       DAOQDO010000601         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27785_length_2906_cov_3.303402, whole genome shotgun sequence.
ACCESSION   DAOQDO010000601 DAOQDO010000000
VERSION     DAOQDO010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27785_length_2906_cov_3.303402"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PKZ53_10535"
     CDS             complement(<1..1645)
                     /locus_tag="PKZ53_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40916.1"
                     /translation="MSLLVLMAALGLGSFGLGDWLCLVQAQPRMRGIGSQPGFRVIGR
                     LVQTELPENIVTTTDFLCESGLADPKNGEYRDIEITTATVWGNSGPTKTHGWVLPSGS
                     NPDIPRVAIAWNGQLYPVLAIGEKASLADDIKQLLDQDKALRQKFKQTSPTAVFSRPT
                     YATPESQAVSTQELTPLKMCLLLRVGERELATQVWNVWKEGVTPEANQTSGSATDPFL
                     RLAGDWLWMRFDRGINAHLRGEDDLARTIFVELETIVPRIEAEARRRGLLPTGETHAG
                     QASSFFPFLKQIPALVRDHARRLTDHPASSGAASSLQARLEALPDKLAQITALVADLD
                     LVAARQKAAGAGVSLTEDPIVRALYRQGEDAVPALLNVIETNDPRLTRSITFYRDYDR
                     DRRALSVQEVAYFLITRLLEIDFGSGSELVIGTAESRTALVTHLREYWSKAKSLTAPE
                     RWFQVLADDTASPDQWLQVVENIVSRDLPSSVPDEMTAGRALRGAVLRAKASPSVSDL
                     VARRVESLMEQFQTSEEFGVLEQATQLVLFLTEWDEASAR"
     gene            1724..>2906
                     /locus_tag="PKZ53_10540"
     CDS             1724..>2906
                     /locus_tag="PKZ53_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40917.1"
                     /translation="MKNNRFVIGWMLFTISFNILTAIAQTSQPPAQQLPSPYKIAFDA
                     RQTLDPGKLLVAMTELRHQENAYLNSKLASSYIQNEAWYCSLTGDYQQAIAWMDSMQQ
                     PPKPKSRDDIAKQAAVLENFKPVDAIKAIETIADQHQVIMINEAHHVALHRQFMIDVL
                     KVLRRKGFTHFAAETFDHLEMTGLSKRGFPLYTKTGFYSDEPVFGNLIRTAVQLGYIP
                     IGYEELSPCEFDAADPERCIRERETGQAKNLKDRILVKNPKAKIVIYAGYAHIQKGGF
                     SATAPSMAQKFQEFTGITPFTIDQIKMMEHSGPDFEVAVYRYAVEAGLVKTPTVFQNS
                     EAQFFVSASSKANFDMQVFHPRTGYRQSRPDWLLRDKSKHLWTIPEDWLKSSLPVLIQ
                     AF"
BASE COUNT          723 a          789 c          739 g          655 t
ORIGIN      
        1 gtcgggccga agcctcatcc cattccgtca aaaacagcac cagttgggtg gcttgctcca
       61 gaacgccaaa ttcctcgctt gtctgaaatt gctccatcag cgattccacc cgacgcgcaa
      121 ccagatccga caccgatggt gaagctttgg cacgcaggac cgctccgcgt aaggctcgcc
      181 cagcagtcat ttcgtcaggt acacttgagg gtagatcgcg ggaaacaata ttttcaacaa
      241 cttgcagcca ctggtcaggc gaggccgtat catcagccaa cacttgaaac cagcgctctg
      301 gcgccgtcag tgatttggcc ttgctccagt actcccgcag gtgagtcacc agcgccgtgc
      361 gggattcagc cgttccgatc accagttcag atccagaacc gaaatcaatt tccagaaggc
      421 gagtgatcag gaaataggcc acttcctgaa cactcagggc tcgccggtct cgatcatagt
      481 cccggtaaaa cgtgatggac cgcgtcagac ggggatcatt ggtttcgatg acattgagca
      541 acgccggaac agcgtcctct ccctgccggt aaagcgctct gacaatcgga tcttccgtga
      601 gtgaaacgcc tgcaccagcc gctttctgac gggcagcgac caggtcaagg tcagccacca
      661 gggctgtgat ctgtgccagt ttatccggaa gtgcctccaa ccgggcctga agtgaactgg
      721 ccgccccgga actggctgga tggtcggtca gcctccgagc gtgatcacgt accagcgccg
      781 gaatctgctt cagaaatgga aaaaatgaac tggcttgacc agcgtgggtc tcgccagtgg
      841 ggagcaaacc tcgccgccgg gcttcggcct cgatacgggg tacaatcgtt tctaattcga
      901 caaagattgt gcgcgccagg tcatcctcac cacgcaggtg ggcattgatg ccgcgatcaa
      961 accgcatcca gagccagtca ccagccagcc tcaaaaatgg atccgttgcg gaacctgaag
     1021 tttgattggc ctctggggtg acaccttctt tccagacatt ccacacctgc gttgccagtt
     1081 cccgttcccc aacgcggagc agaagacaca ttttgagtgg cgtcaattcc tgggttgaaa
     1141 cggcctggga ctctggagtc gcgtaggttg gacgggagaa aacggctgtc gggctggtct
     1201 gcttgaattt ctggcgtagc gctttgtcct ggtccagcaa ctgtttgata tcatcggcaa
     1261 gcgaggcttt ttcaccgata gccaggaccg gatacagttg gccattccag gcaatggcaa
     1321 cccgtggaat gtctgggtta gaaccggacg gcaaaaccca gccatgggtt ttggttgggc
     1381 cgctattgcc ccagacagtg gccgtcgtga tttcaatgtc acggtattcg ccgtttttcg
     1441 gatcagccag gccagattca catagaaaat cggtagtggt aacgatattc tcgggaagct
     1501 cagtttgaac cagccttccg atgaccctga aacctggttg tgatccaata cctctcattc
     1561 ggggttgagc ctggaccagg cagagccagt ctcccagacc aaaactccca aggccaagtg
     1621 cggccatcaa tacaagaagg ctcacgcggt atccaatcaa accagtgctc ctgcctggta
     1681 tccacaattg ataacttgct gaaataaaag gtgttctttt catatgaaaa acaatcggtt
     1741 cgtgattggc tggatgctgt tcaccatttc cttcaatatt ctgactgcaa ttgcccaaac
     1801 cagtcagcca ccagcccagc aacttcccag cccgtataaa attgcgtttg atgcccgtca
     1861 gacactcgat cctggcaagt tactggtggc gatgactgaa cttcgccatc aagaaaacgc
     1921 ctatttgaac tcaaagctcg cttcgtcata tatccaaaat gaagcctggt attgcagcct
     1981 gactggtgac tatcaacaag ccatcgcctg gatggactcg atgcagcagc ctccgaaacc
     2041 aaaatcgcgt gacgatatcg ccaaacaggc ggcggttctg gagaacttca aaccggtgga
     2101 tgcgatcaaa gcaattgaaa cgattgccga ccagcaccag gtgataatga tcaacgaagc
     2161 ccaccacgta gccctgcacc ggcagtttat gattgatgtg ttaaaagtgc tgcgacgaaa
     2221 aggatttacc cacttcgccg ctgagacatt cgatcatctt gaaatgactg gtttatccaa
     2281 acggggcttc ccgctctata ccaaaactgg attttactcg gatgaaccag tttttggaaa
     2341 tttaattcga acggcggttc aattgggcta cattccgatt ggctatgagg aattgtctcc
     2401 ctgcgaattt gatgctgcag atcctgaaag atgtatcagg gagcgggaaa ccgggcaggc
     2461 gaaaaacctc aaggatcgga ttctggtcaa aaacccaaag gccaaaattg tgatttatgc
     2521 ggggtatgcc catattcaaa aaggtgggtt ctcagccacg gcgccgagca tggctcagaa
     2581 atttcaggaa ttcaccggca tcacgccgtt taccattgac cagattaaga tgatggaaca
     2641 cagcggccca gactttgaag tcgccgtcta tcggtatgcc gttgaagccg gactcgtcaa
     2701 aacaccgact gtttttcaaa attcagaggc acagtttttt gtgagcgcca gttccaaagc
     2761 caatttcgat atgcaggtgt ttcatcctcg aaccggctat cggcaaagcc gtccggactg
     2821 gttgctacga gacaaatcca aacatctgtg gacaattcca gaggactggc tcaagtcatc
     2881 gttaccagtt ctaattcagg catttc
//
LOCUS       DAOQDO010000602         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27850_length_2902_cov_3.722866, whole genome shotgun sequence.
ACCESSION   DAOQDO010000602 DAOQDO010000000
VERSION     DAOQDO010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27850_length_2902_cov_3.722866"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="PKZ53_10545"
     CDS             complement(<1..1485)
                     /locus_tag="PKZ53_10545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNJ40918.1"
                     /translation="MESIFGPKGLLARHHPNFEYRSNQIEMAEAVHDALQRGGHLCVE
                     AGTGTGKTLAYLIPALALGKRVIISTATKSLQEQLYQKDIPFLEKALGRKLRVAYLKG
                     RSNYLCLRRIKQSDTALTFAGVEDVTYFDTVRRWSGKTETGDRAELVDLPENLAFWHD
                     IDARSEICLGSKCPDFEKCFVTLARQRAEDADVVIVNHHLFFADLSARNNDYGAVLPD
                     YTIVIFDEAHEIEDIAAEYFGIQVSNYRMQELVQDILRVPIPQDDVVTDVMRCGARIT
                     EQADMFWGGFFRKYGTGDGRFTLEMTHFARRNEEGKFEMTPAGEQYLILDNLLAKLRE
                     LLKKVKEPPPEIERLVRRIDALRFDLEFVVTSNDPTFVYWIERRGRGCFIQATPINLS
                     ELLSDRLFTQMESVVLTSATLTSSGKFDFIRSRLGIESCAELIIDSHFDYRTQSILYL
                     PKIMPDPRSPQFTAAAVEEIVKLLNISHGRAFVLSTSAQHMRELF"
     gene            2016..>2902
                     /locus_tag="PKZ53_10550"
     CDS             2016..>2902
                     /locus_tag="PKZ53_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNJ40919.1"
                     /translation="MKERFQGCLLGVALGDALGAPAKGLTKEEIQTQRSLPVRAAEAS
                     THGHDPVSSPQLPWKMAGGTDIDECNLMMLTLESICTQRSIKTSDMVRCLKGFYRTHP
                     QTLTPLTAHVLSRLNEGERWEEASEGASLDSDFNPPDGGNLPRTIPVGLYRARNIEKL
                     VADTIEVSRLTHWDDRATHSAVAINYLITRLAQNDKQALNSLDTFLADKNAGVRAAVK
                     KGAEGAEADLDTSGLATSVVTVAVWALKNAPSFLEGLEMVINLGGQTDLNAAVAGGLL
                     GAKFGRLVLPEAWLFQLEG"
BASE COUNT          765 a          767 c          711 g          659 t
ORIGIN      
        1 aaacagttcg cgcatgtgct gggcgctggt tgaaagcaca aaggcccgtc cgtgactgat
       61 gttgagcagt ttgacgattt cctcgaccgc tgctgccgta aactgaggag agcgcggatc
      121 aggcataatc ttcggaaggt acaaaattga ctgtgtgcgg tagtcaaaat gtgagtcaat
      181 gatcagttcg gcacacgact caatgcccag acgggaacga ataaagtcaa attttccaga
      241 tgaagtcagg gtagctgatg tcaaaaccac tgattccatt tgggtaaaga gccgatccga
      301 aaggagttct gacagattga tcggtgtggc ctggataaaa catccacgcc cgcgtcgctc
      361 aatccagtag acaaaagttg ggtcattact cgtaacgaca aactcgaggt caaaccgcag
      421 ggcatcaatc cggcgcacca gacgttcaat ctccggcggt ggctccttca cctttttcag
      481 gagttcgcgc aatttggcca gcaggttatc gagtatcagg tattgctcgc cagcaggtgt
      541 catttcaaat ttgccttctt cattccggcg ggcaaaatgg gtcatttcca gcgtaaagcg
      601 cccatcaccg gttccgtatt tgcggaaaaa cccgccccaa aacatatccg cctgttcagt
      661 aatgcgtgcc ccacaccgca taacatcagt cacaacatca tcttggggaa tcggcacccg
      721 taaaatgtcc tgaaccagtt cctgcatgcg gtagttgctg acctgaatgc cgaaatattc
      781 agccgcaata tcttcaattt cgtgggcttc gtcaaaaatg acaatcgtgt agtcaggcag
      841 caccgcgcca taatcgttgt tgcgagccga caaatcagca aaaaacaggt gatggttgac
      901 aatcaccacg tcggcatctt cagcgcgttg ccgggcaagg gtgacaaaac atttttcaaa
      961 gtcagggcat ttcgaaccca aacaaatttc gctccgggca tcaatgtcgt gccagaacgc
     1021 cagattttca ggtaaatcaa ccagttccgc ccggtcaccg gtttctgttt tgcctgacca
     1081 gcgccggacg gtatcaaaat acgtcacatc ttcaacgcca gcaaacgtca gcgccgtgtc
     1141 agattgtttg atccgacgca aacagaggta attgctccgt cctttcaaat acgccacccg
     1201 gagcttgcgt ccaagtgctt tttccaaaaa agggatatcc ttttgataaa gctgctcttg
     1261 aagacttttg gtcgccgtcg aaataatcac ccgcttgccc agtgccagcg ccggaatcaa
     1321 ataggccagg gttttgccgg ttccagttcc agcttcgacg cacaaatgcc cgccacgctg
     1381 caacgcatca tgaacggctt ctgccatttc aatctgattt gaacgatatt caaagttcgg
     1441 atggtgacgg gccaaaagcc ctttcggacc aaaaattgat tccatgagtt cctcaaatac
     1501 aggaaaaaag caaagacaag aattcaacgg tccccgattg taccctattg gcaaatccac
     1561 aaaccactgt gtcttggccg cctgggatag acaatcacca aaaatgcgtg atacaagtgt
     1621 ctctcttcac acaccatcta tagcatttca gataaatatt tcctgggagc gccggcatct
     1681 tgccggcatc aatcggttgc ggttcaagca gttgccggca ggatgccggt gttcccaggg
     1741 gggaatcttt ttctgaaaaa ctatagcctg aatcaagcaa taccagtttt tcgtcagtag
     1801 tccagtagtc agtcattgat acgtcagtag tcagtagaca aaacccggac agttgaatcc
     1861 ttcgggttct tgaaaaaaat tattcctaaa tgattcaaat agaactaaat agacttgaca
     1921 ggcacttcaa aatcccaaca ctcgacgact gacgactgac gactgacgac tgacgactga
     1981 tttgacctga ataccacaca gtttttggag gatgcatgaa agaacggttt caagggtgtc
     2041 ttttgggggt agctctcggc gatgccctcg gagcaccagc caaaggtctc accaaagagg
     2101 aaattcaaac ccaacgttca ctccccgtgc gtgcggctga agccagtacc cacggccatg
     2161 acccggtgag tagcccacaa cttccctgga agatggctgg tggaactgac attgatgagt
     2221 gcaatttgat gatgctcacc ctggaaagca tttgcaccca gcgatccatc aaaacatccg
     2281 acatggtgcg ttgcctgaag gggttttatc gaacccatcc ccaaacattg acaccactta
     2341 cagcccatgt gctgtcccgg ctcaatgaag gcgagcgatg ggaagaagcc tctgaaggag
     2401 catcgcttga ttcggatttt aacccaccgg atggtggtaa cctgcctcga acaattcctg
     2461 tcggactcta ccgggcgcgc aacattgaaa aactggtcgc tgacacgatt gaggtctccc
     2521 ggttgaccca ctgggatgac cgggcaacgc attccgccgt ggccatcaat tatctgatca
     2581 cccggctggc ccaaaatgac aaacaggcac tcaattcact cgacaccttc ctcgccgaca
     2641 aaaacgctgg ggttcgtgcg gcagtgaaaa aaggggcgga aggggcggaa gccgacctcg
     2701 atacgtcagg actggccacc agcgtggtta ccgtcgccgt ctgggcgctt aaaaatgccc
     2761 cgtcatttct cgaaggtctg gaaatggtga tcaacctcgg tggacagacc gatttgaacg
     2821 cagccgtggc ggggggcttg ctcggtgcca aatttggacg gctggttctg cctgaagcct
     2881 ggctgtttca gcttgaaggc aa
//
LOCUS       DAOQDO010000603         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27912_length_2899_cov_2.561885, whole genome shotgun sequence.
ACCESSION   DAOQDO010000603 DAOQDO010000000
VERSION     DAOQDO010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27912_length_2899_cov_2.561885"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKZ53_10555"
     CDS             complement(<1..267)
                     /locus_tag="PKZ53_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HNJ40920.1"
                     /translation="MLSNEYHFITQWCVDGTVQEVSDVIGDVTGLVRWWPSVYLDVMV
                     VEPGNSEGVGKLVELYTKGWLPYTLRWRFRVTESRKPYGFSLEAI"
     gene            555..2798
                     /locus_tag="PKZ53_10560"
     CDS             555..2798
                     /locus_tag="PKZ53_10560"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNJ40921.1"
                     /translation="MTPIDNFFKAVSYTLITSGFATLALTGKVDGFSIVVYLVAIILS
                     WRADKPDSGLQIQATTANRFVLGFLPFLWVDYRYVSNSWILVLIHFFLFVSIFKLFQV
                     KEDRDWVFLYLLAVFEVLLAAGLTIDALFIVCLLVFVMSGLAALEAFEIKRAQQGIRP
                     TKMERAFSHDGLHLKKSARPTRLLIVATGVMGVLIVCLSVPVFFLIPRVNTGLLAYNA
                     PESSITMSGFSDSVELGSVGKIQMSRKVVMYVRVQGSADATRRLRWRGLALSTFTGHS
                     WIKNAFTHKKQHLLPDSRGIFLLDNRPVPLERRSTQTFYLEPMGTDVLFAAGKPLLLN
                     SQFDHLAVDHTNSLYTGFHSAQRLSYTVVAESVALTEEAMRNDPGNYTPDIRAFYLQL
                     PDTFDPRIRQLAQQVVGSAQTPYAKATAIEKYLKSKLTYTLDQTPTPGETDPLIDFLF
                     LRQKGHCEYFASAMTIMLRSVGVAARVVNGFQTGEYNSINNSYIVRQSDAHSWVEVYF
                     PNTQTWSEFDPTPPAGPEATDISFSGYFNRYLDALRLFWIDWVVTYDTDRQKSLAQTA
                     QETVIGYQSKYQLWMKSYRERVSAFLRQFDQVAAGTLHHPMFPAALWSIAFLTVALIL
                     VMQFVRLIRRLRTSPETFFASWWSRWFLVPWIRLTAPKNPQGAAVLFYNEMLSIMARF
                     GWKKPKYLTPLEFAAQTHMPEVLAVTEYYNRVRYGRDEQAALDVKRMQEMLKRVKEAV
                     RQRRKKS"
BASE COUNT          705 a          736 c          682 g          776 t
ORIGIN      
        1 gatggcttcc aatgaaaaac catatggttt tcgcgattca gtcactcgaa accgccagcg
       61 gagcgtatag ggcagccagc ctttggtata taactcaacc agtttgccaa ccccctcaga
      121 attacctggc tccacaacca tcacatccag atatacggaa ggccaccagc gcaccagtcc
      181 agtgacatct ccgatcacat cggaaacttc ctgaacagtc ccgtccacac accactgcgt
      241 gataaaatga tattcatttg acaacatatc tgatttcctt tgatggtttg agtttgagat
      301 gatgcctcgt ctttccgaaa acaactggca cgatagatgt cagcagttgg ggattgcagt
      361 tggattcttt gattgaggct cttacaatca aaggcaggta gcgaagtagt caactcgcga
      421 agtcgcccag gttgacttct gctatgtacc tgtcagtcac taaatatctg aaaagagcgg
      481 ttttgttcca tctcagtctt tcactctttc actctttcac tcttttactt tcttttgacc
      541 ggagcaccac ccatatgacc ccgattgata atttttttaa agccgtttct tacaccttga
      601 tcacctctgg gtttgccaca ctggcactga cgggaaaagt tgacgggttt tcaattgtgg
      661 tttatctggt cgcgatcatt ctcagttggc gggctgataa acctgattcc ggactccaaa
      721 ttcaggcaac cacggccaat cggtttgtgc tggggttttt gccctttctg tgggttgatt
      781 accggtatgt gagtaattcc tggatcttgg tgttgattca ctttttctta tttgtctcga
      841 ttttcaaact cttccaggtc aaggaagacc gagactgggt ttttctctat ctccttgctg
      901 tatttgaggt tctcctggcg gcgggattaa cgattgatgc cctctttatt gtctgccttc
      961 tggtgtttgt aatgtctgga ttggcagcgc tggaagcctt tgaaattaaa cgggcacagc
     1021 aggggatccg cccaaccaaa atggaacgcg ccttttcaca cgatgggtta cacctcaaaa
     1081 aatcagccag gccaacccgc ctgctcattg ttgcaacagg tgtcatgggc gttctgattg
     1141 tgtgtctgag tgtgccagtt ttctttttga taccacgggt gaatacaggg ttactggcat
     1201 acaatgcccc agagtcatca atcaccatga gtggatttag cgattcggtt gaacttgggt
     1261 cggtcggaaa aatccagatg agccgaaaag tagtcatgta cgtccgggtt cagggatcag
     1321 ccgatgccac ccggagatta cgctggcgcg gacttgcact ttcgaccttt actggccata
     1381 gctggatcaa gaatgccttc acccataaaa aacaacacct tctccccgat tcacgaggaa
     1441 ttttcttgct ggataatcgg cccgtaccgc ttgagcgccg gagcacccag acattttatc
     1501 tggaaccgat gggcactgat gtactgtttg cagctggcaa accactcctg ctcaatagcc
     1561 agtttgatca tctggctgtt gaccatacca attctctgta taccggtttt cactcagcac
     1621 aacgtctttc ctatacagtc gtggctgaat cggtggcatt gaccgaagaa gcaatgcgaa
     1681 atgatccagg taattatacc cctgatattc gggccttcta tcttcaactc ccagatacgt
     1741 ttgatccccg tattcgtcaa ctggcgcaac aagtcgttgg ttcggcgcaa acgccatatg
     1801 ccaaagccac cgcaattgaa aaatatctaa aatccaaact cacctatacc ctcgaccaga
     1861 ctccgacccc tggtgaaaca gacccactca ttgatttttt gttccttcgc cagaaaggac
     1921 actgcgaata ttttgcttca gccatgacaa ttatgctgcg gtcagtcgga gttgcagccc
     1981 gcgtggtcaa tggatttcaa actggggagt acaactcgat caacaacagc tatatcgttc
     2041 gtcaatcaga tgcccactcc tgggttgaag tctatttccc gaatactcaa acctggagtg
     2101 agtttgaccc aacaccgccg gctggacccg aggccacaga catcagtttc tctggctatt
     2161 ttaatcgcta tctggatgcc ttgcgcttgt tttggattga ctgggttgtg acgtatgaca
     2221 ctgaccgaca gaaatcactg gcccaaaccg cccaggaaac agtcatcggg tatcaatcca
     2281 aatatcaact ctggatgaag tcataccgcg agcgggtttc tgcatttctt cgccagtttg
     2341 atcaggttgc ggcagggaca ctccaccatc caatgtttcc agccgcactc tggtcaattg
     2401 cctttctgac agtcgccttg attcttgtta tgcagtttgt tcgactcatc cgccggttga
     2461 gaacatcacc tgaaaccttt tttgcttcct ggtggagccg ctggttcctg gttccctgga
     2521 ttcgattgac ggccccaaaa aacccacagg gtgcagccgt gctgttctac aatgaaatgc
     2581 tgtccatcat ggcgcgcttt ggctggaaaa agccaaagta tttgacaccg cttgaatttg
     2641 cggcccagac gcatatgcct gaagtgctgg ccgtcaccga gtattacaat cgggttcggt
     2701 atggacgcga tgaacaggcg gcacttgacg tcaagcggat gcaggaaatg ctgaagcgcg
     2761 taaaagaagc cgtccggcaa cggcggaaga agagctgaaa aatctcgggc tgaaaaactt
     2821 cgggctgaag aaaacgggct cagggcttgg ggcaatcgaa gggatgaggg atgaaggatg
     2881 agggatgaac taaaaccaa
//
LOCUS       DAOQDO010000604         2895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_27979_length_2895_cov_3.345423, whole genome shotgun sequence.
ACCESSION   DAOQDO010000604 DAOQDO010000000
VERSION     DAOQDO010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27979_length_2895_cov_3.345423"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PKZ53_10565"
     CDS             <1..1856
                     /locus_tag="PKZ53_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40922.1"
                     /translation="VIFFIPHPSSLIPSETPDPYPMIQLPTALQPWAEHLELFPAELQ
                     LSLGQLVRRLDQLIGPLHVNTHQLSGEPDGFDGIVRRGSYERLLLSEWVLADEVPDEF
                     IRRAAVGEHSFFNLARRSPAGATVSVALFDAGPNQLGQPRLVHLAALIVLARRAAQAR
                     TPFQWGIFQSPETPLLTGVTEASVLTLLRARSHREITEQDLETWFSTLGISSAPEDFW
                     LVSGPRSRQFSRLSNVSRLEVNDLVSTPNRVLVTIATRTRTIPEIELELPDDQASVRL
                     LRDPFQVSRLPSKLVLGSQYVPASNFVFSFNHTKLFARSTQGELIAYPIPNSPRAPVG
                     KPRLYHSKSKLTIAAVGRIKKTMWILTSTGKVISSESLRGGWDGFLDTDFAVPDWVEP
                     FQTPVNAGPLHQIFYLTSPQAELLTLDGEGRLFSLTLQNGQGQAALHGPYVKAITQLG
                     SKIIYIRQLQKKGWGVVSVETDQKFTHIVEGEGNQAFFGFGYGDGDSKVGLFAAHRGG
                     QEWVVMNRTGEQIYRQPTGTTVIGIIGPIARSNRETPALVVLDDDRRTIHLLGRNWSH
                     SLPRFAHQIDQVTLSTTHPMMAYSTVAGEVGLYSFFDEKVFTLLRGVEKSG"
     gene            2032..>2895
                     /locus_tag="PKZ53_10570"
     CDS             2032..>2895
                     /locus_tag="PKZ53_10570"
                     /inference="COORDINATES: protein motif:HMM:NF039753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bpX6 domain-containing protein"
                     /protein_id="HNJ40923.1"
                     /translation="MMTNSLYPRNLVHRGVVEAAGILIPINLCGVAEAQRRVIGHWET
                     GARVFRVAGGYLVQFSASRLIDCRLADGSPVVALGKTLSTVPLTSQELKTLEPQPHSL
                     ITAQGGQIHIEPLAEQKLETISTWIDLSNFQAVEVTSLGSPPPAVIEAAPALAFDPRQ
                     QLDGVPPASPQMAEVIAALTRPVRVQSAPPSTATTSTQSQSPGFLARLLAQIKLLTTF
                     LSSKEVTTSATARSNSAKSGSKTSPQPSALSARPWWRQLADTIGQLFGSKQSEPPDDQ
                     LRVLPPAPQSML"
BASE COUNT          629 a          862 c          755 g          649 t
ORIGIN      
        1 gggtgatttt tttcatccct catccttcat ccctcatccc ttccgaaacc cctgatccct
       61 accccatgat ccagcttcca actgcccttc aaccctgggc cgaacacctc gaattgtttc
      121 cggctgaact ccaattgagc cttggtcaac tggtgcgccg cctggatcaa ctcattggac
      181 cgctacacgt caacacccat cagttgagtg gcgaaccaga tggctttgac ggcatcgttc
      241 gtcggggaag ttacgaacgg ctgctgctgt cagaatgggt gctcgcggat gaggtcccgg
      301 acgaatttat ccgccgggcg gcagttggtg aacattcatt tttcaacctt gcccgccgct
      361 ctcccgccgg ggccacagtt tcagtggcgt tgtttgatgc cggccccaat caacttggac
      421 aaccacggct ggtccatctg gcagcattga tcgttttagc ccgtcgcgcg gcccaggccc
      481 gcacaccgtt tcaatgggga atttttcaat ctccggaaac gccgctcctg actggcgtta
      541 ccgaagccag cgttctgacg ctgctccggg cgcggagcca ccgcgaaata accgaacagg
      601 acctggaaac gtggttttca acccttggga tctcatccgc acctgaagat ttctggctgg
      661 tcagcggtcc ccgaagtcgc caattttcgc gactgtcaaa tgtttccagg ctggaagtca
      721 atgatctggt cagcacaccc aaccgggttc tggtcacgat tgcaactcgc acccggacga
      781 tcccagaaat cgaacttgaa ctacctgatg accaggcatc ggttcgcctg ctgcgcgacc
      841 catttcaggt ctcgcggcta ccatccaaac ttgttctagg gagtcagtat gttccagcgt
      901 caaattttgt ctttagcttt aaccacacca agctgtttgc ccgctcaaca cagggcgagt
      961 tgatcgccta tccaattccg aattcaccac gcgccccggt tgggaaaccg cgcctctatc
     1021 acagcaaatc caaattgacg attgccgcgg ttgggcgcat caagaaaaca atgtggattc
     1081 tgaccagcac cggaaaagtc attagctcag agtcgttgcg tggcggatgg gacggctttc
     1141 tggatactga ttttgcggtg ccggactggg ttgaaccgtt tcaaacacca gtaaacgccg
     1201 gcccactcca tcagattttt tacctgacca gcccacaggc tgaactcctg acgctggacg
     1261 gggaggggcg acttttttcc ctgacgctcc agaacggtca gggacaggct gcactgcacg
     1321 ggccgtatgt caaagcgatt actcaacttg gctcgaaaat tatttatatt cgccagcttc
     1381 agaaaaaagg ctggggcgtg gtgtcggttg aaaccgatca gaaattcacg cacattgtcg
     1441 aaggcgaagg caaccaggcg tttttcggat ttggctatgg tgatggagat tcgaaagtcg
     1501 gactcttcgc cgcccatcgg ggcggccagg aatgggttgt tatgaaccgc acgggagagc
     1561 aaatctatcg ccagccaacc ggtacgacgg tgattgggat catcggcccc attgcccggt
     1621 caaaccgtga aactccggcc ctggtggtgc tcgatgacga ccggcgaacg atccatctcc
     1681 tgggcagaaa ctggagtcac agcctgccac gctttgccca tcagattgat caggtcacgc
     1741 tttcgaccac gcacccaatg atggcctatt caaccgtggc cggcgaagtc ggactgtatt
     1801 cattttttga tgagaaggta tttacgttgc tacgcggggt tgaaaaatcg ggctgaagaa
     1861 accgggctga gggctaaggg ctgagggctt ggggtattaa acccgttttc ttctgccccg
     1921 agcttgcgag tcttcagccc ccagccccaa gcccgacgtc ttcagccccg agcttgcgag
     1981 tcttcacccc ctggcccgac gtcttcagcc cctagccctg agcccgagtg tatgatgacc
     2041 aactcccttt atccccgaaa cctggttcac cgtggtgtgg tcgaggcggc ggggatcctg
     2101 attccaatca atttgtgtgg tgtcgccgaa gcccagcggc gggtcattgg ccattgggaa
     2161 accggggcac gtgtgtttcg cgtggctggg ggctatctgg tgcaattttc ggcttctcgt
     2221 ctgattgatt gccgtctggc agatgggtcg ccggtggttg cgctgggaaa aacactgagc
     2281 accgttccac ttaccagcca ggaactcaaa actcttgagc cacagcctca ttcgctcatc
     2341 acggcccagg gcgggcaaat tcatattgaa ccgctggccg aacaaaagct ggaaaccatt
     2401 tcaacctgga ttgacctgag caactttcaa gcggttgaag tgacatcgct gggaagtcca
     2461 ccgccggctg tcattgaagc cgctccggca ctggcctttg acccccggca gcaactcgat
     2521 ggcgtcccgc cggcctcacc acaaatggct gaagttattg ccgcactgac gcgaccggtc
     2581 agagtgcaat cggcgccacc ttccaccgca acaacttcaa cacagagcca gtcaccagga
     2641 tttttggcac gcctgcttgc tcaaatcaaa cttctgacaa ctttcctgag cagcaaagaa
     2701 gtcaccacat ctgctacagc ccgttcaaat tcagccaaat cgggatcaaa aacatcacca
     2761 cagccatctg ccctttcggc tcgtccctgg tggcggcaac tggctgatac aatcgggcaa
     2821 ttgtttggaa gcaagcagtc cgaaccacct gatgatcaac tccgggtgtt gccaccagcg
     2881 ccacaatcaa tgctc
//
LOCUS       DAOQDO010000605         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28060_length_2891_cov_3.231664, whole genome shotgun sequence.
ACCESSION   DAOQDO010000605 DAOQDO010000000
VERSION     DAOQDO010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28060_length_2891_cov_3.231664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..>2891)
                     /locus_tag="PKZ53_10575"
     CDS             complement(35..>2891)
                     /locus_tag="PKZ53_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40924.1"
                     /translation="ESHDKSLWISTELGGLMQFKDRQWRIYTHADGLPDGGINTVAED
                     QFGALVVGTEKGLMKLENNRFRPVSRPDGTGIPQINTLLRDGNRLWAGTAAGLYRIEN
                     NQVEFESSIPPIQVTTLLLDQEGHLWVGGFDELIHRSPLGEIQRLVPPGRQHPTFRWI
                     SHVIQTRSGILWAATDLGLFQIQKHLTEYRLVFTETVPQLNVLSLMEDHEGTLWAGSS
                     GNGLFSFRNGVVSAYTATEGISDQEIKTVGGDRRGNIWVGVGDQLKWIAPDKQISALP
                     LPDNLKVSDIFALCEDLETNLWIGTAQGVLRFKDGRFTRFTEADGLSNNNVNAISLGP
                     TGTLWVGTWRGFSRWDNGRFVSFIPNETIAGNWIRSILETPDGTIWLATDNGLTQFQN
                     GRFTRFTTRDGLSDNLTRSLFLDNDGTVWVGTYGGGLNRLKDSRFTQFTPSNGLFDNV
                     VSAIFEDSDGSFWLSGNRGICRVSKRELNDYADGKRTSISSVGYDKADGMAVSETNGG
                     FQPAGWKATDGTLWFPTLRGVVSVDPGKLNRVPPVVRIERLTIDGTSRLPQDHLMFEP
                     GQTKSVEIHYTGLSFAVPSKVKFRYQLEGWDPTWVEAGTRRTAYFTSLPPGNYRFRVV
                     ACNNDSIWNETGATLTFTIKPRFYQTTWFYAGCGLTLFGFGLGIQALRVRQIRRRAEI
                     LERIVRQRTETLRQEKEKTEVARLEADIQRRAAEAADHLKTELLSIAAHDLKSPLYPI
                     KGFASITQDTLQHIDQARQCDEYLSFIDSESQRLLNLINALLDMAALESGKVRLEKKQ
                     VDLSTLVKSAISTFQPQAAAKHQTLNVTTTGHCQIEGDAERLSQVLENLLSNAIKFTP
                     PGKEIEIALECCAHLVRTSIKDAGPGLTADDQTRLFTRFQRLSARPTGGESSTGLGLS
                     IVKDLIELHGGRVGAKNHPHGGSIFWFELPRGMKNEE"
BASE COUNT          677 a          757 c          728 g          729 t
ORIGIN      
        1 cattcttcat tctcaattct tcattctcaa ttcttcattc ttcattcttc attcctctcg
       61 gaagctcaaa ccaaaaaatg cttccaccgt gaggatgatt tttcgctccg acgcgcccac
      121 cgtgcaattc aatcagatct ttgacgatgg aaaggcccag cccggttgat gattcgccgc
      181 cggttggacg cgcactcagt cgttgaaagc gggtaaagag ccgcgtttga tcgtctgcgg
      241 tgagtcctgg gccagcgtcc ttgatggagg ttcggaccag gtgggcacag cattcaagcg
      301 cgatctcaat ttctttgcca ggcggtgtga atttgatggc attgctcaat aaattttcca
      361 gaacctgact caatcgttct gcatcgccct cgatctggca atgtccagtt gttgttacat
      421 tcaaggtttg gtgtttggct gcggcttgcg gttgaaatgt ggaaatagct gatttgacaa
      481 gagttgataa gtcaacctgt tttttttcaa gccgaacttt cccgctttca agtgctgcca
      541 tatcgagtaa ggcattgatc agattgagca atcgttgaga ctcggaatca ataaatgaca
      601 ggtattcgtc gcactggcgg gcctggtcaa tgtgctgcaa ggtgtcctgt gtaattgagg
      661 caaagccttt gatgggatag agcggacttt tcaggtcgtg ggccgcaatg ctcaagagtt
      721 cagtcttgag atggtcagcc gcctcggcag cccggcgctg gatatcggct tcaaggcggg
      781 cgacttcggt tttttctttt tcctggcgga gcgtttcagt gcgttgacgg acaatgcgtt
      841 caaggatttc agcccgccgc cgaatctgac gcacccggag cgcctggatg cccagcccaa
      901 acccaaacaa ggtcaatcca catccagcat aaaaccaggt cgtctggtaa aagcgtggtt
      961 tgatggtaaa ggtcaatgtc gcgccggttt cattccagat gctgtcattg ttgcaggcca
     1021 cgacccgaaa gcgataattc ccaggcggca gactggtaaa ataggctgtt cgtcgcgtgc
     1081 cagcctcaac ccaggttgga tcccatcctt cgagctggta tcgaaatttg actttactgg
     1141 ggacggcaaa actgagcccg gtgtaatgaa tttcaactga tttggtttga cctggctcaa
     1201 acatcaggtg gtcttgaggc aaccgcgaag tgccatcaat ggtaaggcgt tcgattcgca
     1261 ccactggggg cacccgattg agttttcctg ggtcaaccga gaccacaccg cgcaaggtcg
     1321 gaaaccacaa ggtgccatcc gttgccttcc agccagccgg ttggaatcca ccattggtct
     1381 cactcaccgc catgccatcg gctttgtcat agccgacact tgaaatcgaa gtccttttcc
     1441 cgtcggcata atcatttaac tcacgtttgg aaacccggca aatgccgcga tttccgctca
     1501 accagaatga cccgtccgaa tcttcaaaaa tagctgaaac cacattgtca aagaggccat
     1561 ttgatggggt gaactgggtg aaccggctat ctttgaggcg atttaatcct ccaccatagg
     1621 ttccaaccca aaccgttcca tcgttgtcga gaaagagcga ccgggtcaaa ttgtcagata
     1681 gcccatcgcg agtggtgaac cgtgtaaatc ggccattctg aaattgcgtg agcccgttat
     1741 ccgttgcgag ccagatggtg ccatcgggag tttccaaaat ggaccgaatc cagttcccgg
     1801 caattgtttc gtttgggata aacgacacaa accggccatt gtcccagcgg ctgaatccac
     1861 gccaggtccc gacccagagg gttccggttg gaccaagcga aatggcattc acgttattgt
     1921 ttgagagccc atcggcttcg gtaaagcgag taaaccgtcc gtctttgaac cgcaacacac
     1981 cttgagcggt tccaatccag agattggttt ccagatcttc gcaaagggca aaaatatctg
     2041 agactttaag attgtctggc agcggcagag cggaaatctg tttgtccgga gcaatccatt
     2101 tgagttggtc gccaactccc acccagatat ttccgcgacg atctccgccg acggttttga
     2161 tttcctgatc ggaaataccc tcggttgcgg tgtaggctga aacgacacca tttcgaaagc
     2221 taaacaatcc attgccactg gagccagccc acagcgtccc ttcgtgatct tccatcagtg
     2281 acagcacatt gagctggggg actgtttctg tgaagaccag gcgatattca gtcaaatgct
     2341 tctgtatctg gaacaaccct aaatcggtgg cagcccacag gatacctgac cgggtttgaa
     2401 tcacgtgact gatccagcga aacgtagggt gttgccttcc cggaggcacc agacgctgaa
     2461 tttccccaag cggagagcgg tgaatcaatt cgtcaaaacc accaacccac aggtggcctt
     2521 cttgatctaa aagtagggtt gtgacttgaa tcggaggaat ggaggattcg aactcaacct
     2581 ggttgttttc aatgcgatag agtccagcgg cagtgccagc ccataacctg ttcccatcgc
     2641 gcaacagtgt attgatttgt ggaattccgg ttccatccgg tctggaaact ggtcgaaatc
     2701 ggttgttttc caatttcatc agcccctttt ccgtaccaac cacaagtgct ccaaattggt
     2761 cttccgccac tgtgttgatg cccccatccg gtaacccatc cgcgtgagta taaatcctcc
     2821 actggcggtc tttgaactgc atgagcccgc cgagttcggt tgaaatccat aaacttttgt
     2881 cgtgggattc a
//
LOCUS       DAOQDO010000606         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28210_length_2883_cov_2.812235, whole genome shotgun sequence.
ACCESSION   DAOQDO010000606 DAOQDO010000000
VERSION     DAOQDO010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28210_length_2883_cov_2.812235"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..649)
                     /locus_tag="PKZ53_10580"
     CDS             complement(368..649)
                     /locus_tag="PKZ53_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40925.1"
                     /translation="MALEGISASESPTISSAVINGKQLIVTGSNFDSGASLLVNGETQ
                     KKTTNDATNPTSILIAKKAGKKIASGQTVTLQVRNPGGLISSEFQFTRP"
     gene            complement(638..1390)
                     /locus_tag="PKZ53_10585"
                     /pseudo
     CDS             complement(638..1390)
                     /locus_tag="PKZ53_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
     gene            complement(1764..2582)
                     /locus_tag="PKZ53_10590"
     CDS             complement(1764..2582)
                     /locus_tag="PKZ53_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNJ40926.1"
                     /translation="MKKQLLFLFGSVAVLGAVLLSTFLPQTFAFGQHPERRGMKDRRA
                     QFAQELNLTDAQKSEMKTLMAREHQALKPIFQQLSQFQDQIRNVNADGQFHEAEVRPL
                     AEQQAKLMTSLQLRHIETRSQMQKILTAEQLQKAESLRPNFDRGFGPMPFMGAHQREE
                     QMARVLGLTPEQEAQIQTNLEADRPVVSGLLQQLKTQQQQIQTLTTTGADQTQINALL
                     DQQTRTLTDLIVQRERTRLKNDAILTPEQRATAEALHDRMKMMMGERFGRPDVD"
     gene            2703..>2883
                     /locus_tag="PKZ53_10595"
     CDS             2703..>2883
                     /locus_tag="PKZ53_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40927.1"
                     /translation="MATLTFLYRKSGTSRVLSGIGYLVLGDWCFKINPLKKSEEQRTK
                     NQEPRTKNQERRTKNE"
BASE COUNT          718 a          630 c          765 g          770 t
ORIGIN      
        1 ttctgacgga agcacaaaca gatcaccttt ctggaagaat gaagaatgaa gaaagcatct
       61 ggtgcttggt gggactgtcc aaattgttct gtaatcaggc aatttaacct ggaaaaatca
      121 aatggtttgc gcttcttttt agctgtcaag tgttgaatac aaacagcttg cctgaattaa
      181 gaaaagcgag tcggaaattc cagtttggtc atttacacaa aaaattgaaa agactctggt
      241 gcttggaaga cattggttcc aaagcactaa gtaccagtca cctgcctttt cttcattctt
      301 cattctcaat tcttcattct caattcttca ttcttcattc ttcattctca attcttcatt
      361 ccccaggtca ggggcgggta aactggaatt cactcgaaat cagcccgcct ggatttctga
      421 cctgtaaggt aacggtttgc ccggaggcaa tttttttgcc ggcttttttg gcaatcagga
      481 tgctggtggg attggtggca tcgttggtgg ttttcttctg ggtttcgccg ttgaccagca
      541 gggaagcccc gctgtcaaaa ttgcttccag tcacaatcaa ttgtttgccg ttgatgacgg
      601 cactggagat ggttggtgat tcagaggctg aaattccttc aagcgcaata tcaaaagtgg
      661 cgagataccc ttgttcattc attgtaactg gaaggagcaa ctggtctccg ttattgcgat
      721 aaatgccgtc ctgggtgttg cgggtattgc gggttccttt tgaactatag ggtgactggg
      781 aatgcaccag atcggtcacc gtatcgtcaa aatagagttg tgaggtgaat tggtgggtga
      841 cactggaacc gttaaagagt cggattttaa aatggatatg aaccgtgcgc ccggtgtacc
      901 agcccggata aatagtaatg aactcaactg caccattggc atcggtgacc tgatacccac
      961 gcaggaattt ttgaccgacg gtattaaatc cttcgccggt atccagcacg tcggaataaa
     1021 cccccagcgc atcacagtgc cagatatcaa ccaccgcacc aggcaatgga acacaacttc
     1081 cggtcgttgt tcgggtgaca ttgattttta atcggagcgg cgtgccagcc tttaccgtgt
     1141 tattggtggg atccattcga atatcagagc ggttgagcat ttcatcaaca aagtaagggc
     1201 cttcggtttg ttctggcctg acaatacagg acagagcatc tgctttgacg gttgagagag
     1261 gtttggaact ggaccagaat ccaggaggca aggtttccca tccggcgagc atggccactc
     1321 cggtggctcc aaaaagacgc agtagttcac ggcggcttga aaaacggttg gtcaggtgtt
     1381 ttttggccat aaaggattcc tttacaattg ggatgagatg gcggggaatg tgccagtatt
     1441 ttgatttagt cgtctgaagg aattgttgtt tgagccacag gtgcaatctc agtgtgaaaa
     1501 aatcccgctg ccatcagaca gcgggagaga cgtgcgtaga gaagttaact ggaaggaaag
     1561 agtcgtgaaa gagagcggga ggagccggtc aaccggctcc agaagctgtt ttggattcgg
     1621 cctggtacgt tcacaaaaca gcctgtccca actgcaagtt ataccgtttt ggaatgagcc
     1681 tatcgtgtta tgggaaagcg aaatcattga aaaaattgca tttccctcag cccttagccc
     1741 gataccctca gccctcaatg gtattagtcc acgtccggac ggccaaaccg ttcgcccatc
     1801 atcattttca ttcgatcatg gagcgcttca gccgtggccc gttgttctgg ggttaaaatc
     1861 gcatcatttt tgagccgggt gcgttcgcgt tgcacaatca ggtcggtcag ggtgcgagtt
     1921 tgttgatcaa gcagggcatt gatctgagtt tggtcggcgc cagtggtagt cagcgtctga
     1981 atctgttgct gttgggtttt tagttgctgg agcaggcccg aaacaaccgg gcggtcagct
     2041 tcgagattgg tttggatctg ggcttcctgt tctggagtca ggcccagcac tcgggccatt
     2101 tgctcttcac gctgatgcgc gcccataaac ggcattggtc caaagcctcg gtcaaagttg
     2161 gggcggaggc tctcagcttt ctgaagttgt tcagccgtca aaattttctg catttgactg
     2221 cgggtttcaa tgtggcgcaa ttgcaggctg gtcatcaatt tagcctgttg ctcagccagg
     2281 ggacggactt cagcttcgtg aaactgacca tcggcattga cgttgcgaat ttgatcctgg
     2341 aattgagaga gttgttgaaa gatgggcttg agggcctgat gttcacgtgc catcagggtt
     2401 ttcatctctg acttctgagc gtcggttaaa ttcaattcct gagcaaattg agcccgtcgg
     2461 tccttcatgc ctcgtcgctc tggatgctgg ccaaaagcaa aggtctgtgg aaggaacgtg
     2521 ctcaacaaaa cggcacccaa aacggcaacg ctgccaaaaa gaaaaagaag ttgttttttc
     2581 atggttgatt ctcctgcggg gtaagatctg tgattgcctg agccattcac ggtgtattgg
     2641 aagatacgcc agttttgtca gatgtgcggt ctaggtatgt caacggtgcg tcaggatttg
     2701 tcatggcgac gctgaccttt ctttacagaa aaagtggaac cagtcgagta ttatcaggaa
     2761 ttgggtactt ggtacttggt gactggtgct tcaagatcaa tcctttgaaa aagagcgaag
     2821 aacaaagaac aaagaaccaa gaaccaagaa ccaagaacca agaacgaaga accaagaacg
     2881 aag
//
LOCUS       DAOQDO010000607         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28264_length_2880_cov_4.320000, whole genome shotgun sequence.
ACCESSION   DAOQDO010000607 DAOQDO010000000
VERSION     DAOQDO010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28264_length_2880_cov_4.320000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1039
                     /locus_tag="PKZ53_10600"
     CDS             5..1039
                     /locus_tag="PKZ53_10600"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HNJ40928.1"
                     /translation="MRHTITLIPGDGIGPEVTAATLRVIEAAGVEIRWEKYAAGAEAL
                     GQYGTPLPDNLLASIRRTRVALKGPVTTPIGTGFTSVNVGLRKALDLYANLRPVKSLP
                     GVKSRYENIDLIVVRENTEDLYSGLEHVVVPGVVESLKVITEVASTRISRFACDYARR
                     HKRRKVTAVHKANIMKLSDGLFLDCFRKVSQHYPEIEPDEKIVDNACMQLVMYPEKYD
                     VLLMENLYGDILSDLCTGLVGGLGVVPGANIGDNMAVFEAVHGSAPDIAGQGLANPTA
                     LILTANMMLEHIGEGEAAQNIEKALFEVLAEGKQVTRDLGGTASTMQFTEALASRLEA
                     WRKEKADEAK"
     gene            complement(1249..1725)
                     /gene="tsaE"
                     /locus_tag="PKZ53_10605"
     CDS             complement(1249..1725)
                     /gene="tsaE"
                     /locus_tag="PKZ53_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNJ40929.1"
                     /translation="MPNWETGTFHSDSPEETFELGYELGEAMTSEMARVCFLILLEGD
                     LGAGKTCFIKGLAAGLGVDEREVSSPTFTLVNRYEEGRLLFHHVDLYRLPEGTNLLHS
                     LGLEEVLDQPGVLAIEWSERLKGFSWPNGARITFRVVDELRRDIQVERNGSEGAKE"
     gene            complement(1898..>2880)
                     /locus_tag="PKZ53_10610"
     CDS             complement(1898..>2880)
                     /locus_tag="PKZ53_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ40930.1"
                     /translation="GGDFHGLVGHVTLGYRKIEKPDPCLGNVAPTVTLSADKLAVKEK
                     SKDVVTATVTATDPDAADSTLTYRWFATSGDLKGEGTSVTWTPGITAPGNVLITVNVA
                     DSCGHSVNKEVSVIVEKVNHCPTVTVSASPTSIKEGSDQPFTFTAVGKDEDDDKLTYK
                     WTTSRGRIEGRGSKVTVNTQGLSAGTITVSVVANDKTCDSAPASTTVEIIAPPPPPPV
                     RTFTCDTMMFKKNISRIDNQCKAVLDEVASTLQSDPTAVCLIDGHSETGEKAGTAQAR
                     AEKARDYLTSKGIDANRIEVRNFDDSRPDAAGNHRRIIISVVPEGAKRPE"
BASE COUNT          687 a          739 c          729 g          725 t
ORIGIN      
        1 ctttatgcga catacaatca ctttaattcc cggagacgga attggacctg aggtgacggc
       61 ggccactctg cgtgtgatcg aggcggctgg agttgaaatt cgatgggaga aatatgccgc
      121 cggggctgaa gccctgggtc agtatggaac accactcccc gataatctcc tggcctcaat
      181 tcgccgcacc cgagttgcgc tcaaaggccc ggtgacaaca cccatcggaa ctgggtttac
      241 cagcgtcaac gttgggttgc ggaaagcgct tgatttatat gccaacctgc ggcccgtcaa
      301 atcattgccc ggtgtcaaat cccgatatga aaatattgat ctgattgtgg ttcgtgaaaa
      361 caccgaagac ctgtactcag gacttgaaca cgttgtggtg ccaggagttg tggaaagtct
      421 gaaggtgatt actgaagttg cttcgacccg catttcccgc tttgcctgcg attatgcccg
      481 caggcataag cgccgcaagg taactgccgt ccataaagcg aacattatga agctctcgga
      541 tggtctcttt ttggattgct ttcgtaaggt gagccagcac tatcccgaaa tcgaacctga
      601 tgaaaagatt gtggataatg cctgtatgca gttggtgatg tatccagaaa agtatgatgt
      661 cctgttgatg gaaaacctgt atggagacat tctttccgac ctgtgcactg gattagttgg
      721 tggccttggt gtcgttccag gtgccaatat tggcgacaac atggcagtgt ttgaagccgt
      781 tcacggcagc gctcctgaca ttgccgggca gggactggcg aatccaaccg ccctcatcct
      841 gaccgccaat atgatgcttg aacacatcgg cgaaggtgaa gcggctcaaa acattgagaa
      901 agctctcttt gaagtactgg ctgaaggcaa gcaggtgacc cgtgatttgg gtggtactgc
      961 cagcactatg caatttaccg aagccctggc ttcacgtctt gaagcctggc gaaaagagaa
     1021 ggctgacgaa gcgaaataat gaagagcgaa agcgcgaggg agtgaaagat aaagagtttc
     1081 agtgtcgtca gaaaccaaat ttgagatata tagcatttca gataaatatt tcctgggagc
     1141 gccggcatct tgccggcatc aatcggttgc ggttcaagca gttgccggca ggatgccggc
     1201 gttcccaggg gggaatcttt ttctgaaaaa ctataacact gaaagatctc actccttcgc
     1261 tccttcgctc ccatttcgct caacttgaat atccctcctg agttcatcca ccactcgaaa
     1321 cgtaatccgc gcgccatttg gccaggaaaa tcctttcaac cgctcgctcc attcgatggc
     1381 caatacccca ggctgatcga gtacttcttc caacccaagg ctatggagca gattggttcc
     1441 ttccggcagg cgatataaat caacgtgatg aaagagcaat cgcccctctt catagcgatt
     1501 gaccagggta aaggttgggc ttgatacttc gcgttcgtcc acacccaacc cagccgctaa
     1561 ccctttgata aagcacgttt tccccgctcc caaatctcct tccaacaaaa tcaaaaagca
     1621 gactcgggcc atttcagaag tcatcgcctc accaagttca tagcccaact caaaagtttc
     1681 ttcaggcgaa tctgaatgaa acgttccagt ctcccaattt ggcatagtgt gaattctcaa
     1741 ttgaaagaaa aaggtacagc gttcaagctt tcgattgaac tctgtacccg tgacattgtc
     1801 gaacaatgag ggaagtgatg aataaatgat ctgattgcta aaccagtggt ttgcattctt
     1861 cattcttcat tcttcattct tcattctcca ctctgatcta ttcgggccgt ttggcacctt
     1921 ccggaaccac cgaaatgatg attcggcgat gattgccagc cgcatccggg cgcgaatcat
     1981 caaagttacg aacttcaatc cggttggcgt caatgccctt cgaagtcaga tagtcacgcg
     2041 ctttttcagc ccgagcctga gccgttccag ctttttcacc cgtctcagag tgcccgtcaa
     2101 tcaggcacac ggcggttggg tcggactgca aggtgctggc gacttcatca agcactgctt
     2161 tgcactggtt atcaatgcgc gagatgtttt tcttaaacat catggtgtcg caggtgaagg
     2221 tccgtactgg tggtggcgga ggcggtgcaa tgatttcgac tgtggtcgaa gccggtgctg
     2281 aatcacaggt tttgtcgttg gccaccactg atacagtaat cgtgcctgcc gacagccctt
     2341 gcgtattgac ggtgactttg gatcctctgc cttcaatgcg cccacgtgat gtggtccatt
     2401 tgtaggtcaa cttgtcatca tcctcatctt tgccaactgc tgtgaaggta aacggctggt
     2461 ctgaaccttc cttgatcgaa gttggtgacg ccgagaccgt cacggttggg caatgattca
     2521 ctttttcgac gatgaccgag acttctttat tgaccgaatg accgcacgaa tccgcgacgt
     2581 tgacagtgat caacacgttt ccaggagcgg taatgcctgg tgtccaggtg acggatgtgc
     2641 cttcgccttt caagtcgcca ctggtggcaa accagcgata agtcagcgtg gaatcagccg
     2701 catctgggtc ggtcgccgtc accgtggcgg tcacgacatc tttgcttttt tctttgacgg
     2761 cgagtttgtc agccgagagt gtgactgtcg gagccacatt acccaggcag ggatcaggtt
     2821 tttcgatctt gcgataaccc agggtcacat gaccaaccag cccgtggaag tcaccgccat
//
LOCUS       DAOQDO010000608         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28302_length_2878_cov_3.486716, whole genome shotgun sequence.
ACCESSION   DAOQDO010000608 DAOQDO010000000
VERSION     DAOQDO010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28302_length_2878_cov_3.486716"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2106)
                     /locus_tag="PKZ53_10615"
     CDS             complement(97..2106)
                     /locus_tag="PKZ53_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ40931.1"
                     /translation="MTSAVLFSVVMAPSVTAATQKPTKTASPIQRPTPDAPTPREQRM
                     RSYMLYLQARDYEAQGDYPKAINAYKEIIQLDPRAASPHISLSEIYFQNRNLKDAETE
                     ARQAVSLEPENPAGRRLLGNILAVQSLSGVLNKEKVNEAILQFQEVARLDKADPEVYR
                     IMGRLYQATGDADKAIEHFQKLMGSGVAKGPDFELMARLYYEKGRYRDAAQVARQAYI
                     LSDQNLRLGALLAQSLLRSGQTTEALEVYQQILTDNPNQPELLLDYAETLMLAGKYPE
                     ALQGAQKILEMDPTNVQALSILAQTQRRSGRREEAVKVLEQALKGQDVTESLQLQFEL
                     AETLIELGRVEDGVKAYQRALNALLNPDGTVGERDKRNAGVILRRIAMAYREAGQRAK
                     AVETFGYMRKVLGDKDTLPDGMEIDLLRSEGNYAEALKIARDAQKRFPNDRQFKYLEA
                     FSLSKLGEVNKAVELLEKMLGSNAEDADVYQTLGSLLMEAGRLEDAEKAARRALQFDQ
                     KNTESLVLLSSIQDRRKMYKESEETLRQVLAIDPDNPTALNNLGYFLTERGEKLDEAL
                     LLIQRAVNIEPTNSSFLDSLGWLYFKRSQYDQARQYIEQAIGYDGRSATQHDHLGDVY
                     EKLGKLDQAREMWKKAVDMATDPAEIERIKTKLNASQASQKSPNK"
     gene            complement(2269..>2878)
                     /locus_tag="PKZ53_10620"
     CDS             complement(2269..>2878)
                     /locus_tag="PKZ53_10620"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNJ40932.1"
                     /translation="NSSFSILHSSFLTMILYLVGFMGSGKSTIGNALAEKWRIPLIDL
                     DAEIIRAAGCSIPEIFEREGELGFRQRERETLQAVCATQVTPESVPDGQPECVISLGG
                     GAFTQEAIRQQIATTGVSVWLKASFETCAARLRETTDRPLFQSPATAQTLFESRLPVY
                     ALANIEIETDGQPISVICAQIQQAFLGYSSRITPLSSEALDL"
BASE COUNT          688 a          736 c          695 g          759 t
ORIGIN      
        1 gttctttttt ctccaaatag gacgcggcag aaagatagag aggtcaaact ccgttgtgga
       61 aaggttggtt ttccgcaacg gagtttgcac aaagcattat ttatttgggc ttttctggct
      121 ggcttgtgac gcattcaatt tggttttgat ccgctcgatt tcagccggat cggttgccat
      181 atcaactgct tttttccaca tttcacgtgc ctgatccagc tttccaagtt tttcatatac
      241 atcgcccaga tgatcgtgtt gggtggcgct ccgaccatca tagccaatgg cttgttcgat
      301 gtactgacga gcctggtcat actgacttcg tttgaaatac agccagccaa gactatccag
      361 gaacgaactg ttggttggtt caatattgac cgcccgctga atcaggagca gggcttcgtc
      421 aagtttttca ccgcgttcgg tgagaaaata ccccaagttg ttcagagcag tcgggttatc
      481 ggggtcaatc gccaaaacct gccgcaaggt ttcttctgat tccttgtaca ttttgcgtcg
      541 gtcctggatc gaactcagca aaacgagcga ttcggtattt ttttgatcaa attgcagggc
      601 acgacgggcc gccttctcag cgtcttccag tcgtccagct tccatcagga gcgagccaag
      661 tgtttgatac acgtcagcat cttcggcatt gctgccaagc attttttcca ggagttcaac
      721 tgccttgttt acctcgccta acttggacaa tgaaaaggct tccagatact taaactgacg
      781 gtcatttggg aatcgcttct gggcatcacg cgcaattttc aaggcttcgg cataattgcc
      841 ttcagaccgc aggaggtcaa tttccatgcc atctggcagc gtgtctttgt cgccaaggac
      901 tttccgcata tagccaaagg tctcaacagc tttggctcgc tgaccggctt cacgatacgc
      961 catggcaatg cgccgtaaaa taacgccagc attgcgttta tcacgttcac caaccgttcc
     1021 gtccggattc agcaacgcat ttaaggcccg ttgataggct ttgaccccat cctcaacccg
     1081 gcccaattca atcagggttt cagccagttc aaattgcaac tggaggcttt ccgtcacatc
     1141 ctgacctttg agggcctgtt ccagcacttt gacagcttct tcccgtcgtc cgctccggcg
     1201 ctgggtttgg gccagaatgc tcagggcctg gacgttggtc ggatccattt ccaggatttt
     1261 ctgagcccct tgcagcgctt ccgggtattt tccggccagc atcagggttt cggcataatc
     1321 cagcagcaat tcaggctggt tgggattgtc agttaaaatc tgctgataga cctcaagggc
     1381 ttcagtggtt tggccagaac gcagcaaaga ttgggccagc aacgccccaa gccgcaaatt
     1441 ctgatcactt aaaatatacg cctgccgggc cacctgagcc gcatcacggt accggccttt
     1501 ttcataatac agccgggcca tcagttcaaa atccggacct ttggcaacgc cgctccccat
     1561 caacttctga aaatgttcaa ttgctttatc tgcatctcca gtggcctgat acagccgtcc
     1621 cataatgcga tagacctctg ggtcagcttt atctaaacgg gcaacttcct gaaactggag
     1681 gattgcttcg ttcacctttt ctttgttcaa gacacctgac aacgactgaa ccgccagaat
     1741 attgcccagt aaccgccgac cagccgggtt ttcgggttct aaagacaccg cctgacgcgc
     1801 ttcagtttca gcatctttga gattgcggtt ttgaaaataa atctcactga gtgaaatatg
     1861 cggagaggct gcccgagggt cgagttgaat gatttcctta taggcattaa tggctttggg
     1921 gtaatcgccc tgagcttcat aatcacgtgc ctgcagatag agcatatacg aacgcatccg
     1981 ctgttcacgt ggtgttgggg catccggcgt tgggcgctgg attggcgatg ccgttttggt
     2041 tggcttttga gtcgctgctg taacagatgg tgccatcacc actgaaaaga ggacggcgga
     2101 ggtcaaaggg gtggagaact tcatgtgagg tgaaccctgt tcctttactt caatgtgggt
     2161 tgataaaaaa tcttagtata acaaccaggt attgtgccat cggtgaagcc agttacgaat
     2221 tatgaactat aaaaacctaa ccacctgata ttcaatgaga taacaatttc acagatcaag
     2281 agcttcggaa gaaagtggtg taatccggct tgaatacccc aagaaagcct gttgaatctg
     2341 agcgcaaatg acggatatgg gttgtccatc agtctcaatc tcaatgtttg ccagggcgta
     2401 aaccggcaat ctggattcaa ataaagtttg tgcggtggcc ggtgattgaa acagtggccg
     2461 gtcagtggtt tcacgcaatc gagcggcgca ggtttcaaag ctggctttga gccacaccga
     2521 aacacccgtc gttgcaatct gctggcgaat cgcctcctgg gtaaaagccc cgccccccag
     2581 tgaaataaca cattccggtt gaccatccgg cactgattct ggtgtcacct gcgttgcaca
     2641 tacagcctgt aatgtttccc gttcacgctg acgaaatccc aattcgcctt cgcgttcaaa
     2701 tatttccggg atcgagcatc cagcagcccg aataatttca gcatccaggt caatgagggg
     2761 aatccgccac ttttcagcta acgcattccc aatcgtagat ttgccgctgc ccataaaccc
     2821 aaccagataa aggatcatag tgaggaatga agaatgaaga attgagaatg aagaattg
//
LOCUS       DAOQDO010000609         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28309_length_2878_cov_2.822883, whole genome shotgun sequence.
ACCESSION   DAOQDO010000609 DAOQDO010000000
VERSION     DAOQDO010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28309_length_2878_cov_2.822883"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PKZ53_10625"
     CDS             complement(<1..1175)
                     /locus_tag="PKZ53_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNJ40933.1"
                     /translation="MQQSVRFSQSLELALHRVARQVARQLGLDYPPEQWRDLYYRLGL
                     VARDLGLPDAETAIQVLGDLPHFPHSIVDALICHLTITETYFFREKPVFDVLEQEILP
                     DLIRSRQHTGRFLRIWSAGCSTGEEIYSVAILLTRLLPDLAQWNLTLIGTDVNPAVLK
                     QARGGIYRDWSFRMTPEAIRSRFFTRTSDQTYQLDPQVCSLVRFERHNLASDAFPPPL
                     AGPGSFDLILCRNVLMYFAPETRRRLLQQFTQCLTAPGWFFSSLNEVASINTPALERK
                     YSPNAIIFHKSGRELTFIQPALPLNPEPVPPGFEPVLATTLPAAIQAATPRLTSEPVL
                     RLTPARDWQKLPAGGNISATSAHSQSLFEADKLDPVSHFLEAVTQSEQGDTEAALRG"
     gene            complement(1276..1770)
                     /locus_tag="PKZ53_10630"
     CDS             complement(1276..1770)
                     /locus_tag="PKZ53_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ40934.1"
                     /translation="MTRWLSEAFWSGGGILEHEYLVFSIDNRQCAIDLALVERVTPMV
                     TLIPFSGAASPVVGLTNYKGDLLPTVSLRWWWGFPPPFVAPQDFLLVIRHSKQLLVLV
                     VDKVIGVVPIAQDTQLEAHPEGPEWLQHTKIMTNRTTTVYLPDLHRLFQTVQAMNLKN
                     QFLC"
     gene            2165..2734
                     /locus_tag="PKZ53_10635"
     CDS             2165..2734
                     /locus_tag="PKZ53_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNJ40935.1"
                     /translation="MEHQQILIACIGNIFFGDDAFGVEVARALSQRCLPEEVVVVDYG
                     IHGLDLAFALLDGYEAVILVDAVRRGGQPGTLYLIEPDSTDIKASADQSLIIESHRLN
                     PMRVLAFAQSMGARLNRILLIGCEVTSPDLLEEMTMELSPAVQAAIPSAVEFIELLIT
                     KILDEDFDAWYPHATQGKFGSLPISGWKA"
BASE COUNT          750 a          702 c          744 g          682 t
ORIGIN      
        1 aaccctcgaa gcgccgcttc ggtgtcaccc tgttcagatt gcgttaccgc ctccagaaaa
       61 tgggataccg gatcaagttt atcagcctca aacagcgatt gactgtgggc cgaagtggct
      121 gaaatatttc cgccagcggg cagtttttgc cagtcacgcg ctggggtcag ccgcagcaca
      181 ggctcggagg tgagtcgtgg cgtggcagcc tgtatcgcgg ctggcaaggt agtcgccaaa
      241 actggctcaa atccgggcgg cactggttca ggattgaggg gaagcgcggg ttgaatgaaa
      301 gttaactccc gacccgattt gtgaaagata atcgcattgg gtgagtattt gcgctccagg
      361 gccggtgtat tgattgaagc aacttcattc aggcttgaaa aaaaccagcc gggcgctgtc
      421 aggcactgag taaactgctg gagcagccgg cgccgggtct ccggagcaaa atacatcaac
      481 acattgcgac ataaaatcaa atcaaagctg cccggtccgg ccagtggcgg agggaacgca
      541 tcgcttgcca gattgtggcg ttcaaacctg accagtgagc aaacctgagg atctaactga
      601 taggtttggt ctgaagttct ggtaaaaaac ctggatcgga tggcttccgg agtcatccga
      661 aatgaccagt cacgatatat tccacctctg gcttgcttta aaaccgctgg attgacatca
      721 gtgccgatca acgtgaggtt ccattgagcc agatctggaa gtaaccgcgt gagcaatatg
      781 gcaactgagt atatttcctc tccggttgag caaccagcgc tccagatgcg taaaaaccga
      841 cctgtatgtt gccgcgagcg aattaaatcg gggaggattt cctgttccag tacatcaaac
      901 actggtttct cacgaaaaaa ataggtctcg gtaatcgtca gatgacagat caatgcgtca
      961 actatcgagt ggggaaaatg tggcaggtca ccgaggacct gaatggcggt ctcggcgtct
     1021 ggcaacccca gatcacgagc caccagacca aggcggtaat acaaatcccg ccattgttcg
     1081 ggtggataat ccagaccgag ttgccgggcg acctgtcggg caacccgatg gagcgccagt
     1141 tcaagtgact gagaaaaacg aacggactgt tgcattgagc tttccatctt tcaaaaaacc
     1201 agacaggacc aaatgacaca ccaattcaga tttcttcatc tgtgacaggg atttcgccac
     1261 tatctgcctg agcggttaac ataaaaactg attcttgaga ttcatcgcct gaacggtctg
     1321 aaacaggcgg tgcagatctg gtaaatagac ggtggtagtg cgattggtca tgattttggt
     1381 gtgttgtaac cactcggggc cttcagggtg ggcttcgagc tgagtgtcct gagcgatagg
     1441 gaccactccg atgaccttgt caaccaccaa aaccagaagc tgtttggaat gcctgatcac
     1501 caacaaaaaa tcctggggtg ccacaaaggg cggcggaaat ccccaccacc atctcaagct
     1561 caccgtgggc agcaaatcac ctttgtaatt tgtcaatcca accaccggcg aagcggctcc
     1621 agagaaagga atcaacgtca ccatcggtgt tacacgctcg accagtgcca gatcaatggc
     1681 acattgtcgg ttatcaatcg aaaaaaccag atactcgtgc tccaaaatac ctcccccgct
     1741 ccaaaatgcc tcactcaacc agcgggtcat cttttcaacg atcttttcgg gtctaaattg
     1801 tcctggatcc cgactgtcta aagtacaaaa actgtgccac agaaatagag gcttgacggc
     1861 ctgatcgcga acgaaccaac caggctcgat caatcgtgca tctgttcgac gggggcggga
     1921 aagaacgatt ccaaaacagt gaatcctgat ctgctacccg ttgcaggtga tcaaaaaatc
     1981 gtcggtgcaa tggcagcagt gtcaggaata tgacaaaaat tttatatttc ttcaattatg
     2041 aacagctccc aaaatattga caatagtgat tttctgccca caatagagtc aagacgttag
     2101 catttgattg tcttctgccc attatcgttt gaccagaatg acactggttt gaggttgacc
     2161 aggtatggaa caccagcaaa tcctcattgc ctgtatcgga aatatctttt ttggggatga
     2221 tgcctttggg gtcgaagtgg cccgagcatt gtcacagcgg tgtttgcccg aagaagtggt
     2281 ggtggttgat tacggcatcc atgggctgga cctggcattt gcgctccttg atggatatga
     2341 agcagttatt ttagtggatg ctgtccggcg aggtgggcaa ccgggcacac tctatctgat
     2401 cgaaccggat tccactgaca tcaaagcttc agccgatcaa tccttgatca ttgaatcaca
     2461 ccgcctgaat ccaatgcgtg tcctggcttt tgcccagtca atgggtgccc ggctcaaccg
     2521 catcctgctg atcggatgtg aagtgacgtc accagatttg cttgaggaaa tgaccatgga
     2581 gttaagccct gcggttcagg cagcaattcc ttcagccgtt gaatttattg aattactgat
     2641 taccaagatc ctggacgaag atttcgacgc ctggtatccg catgccaccc aggggaaatt
     2701 tgggagtctt ccgatttcag gctggaaagc ctgacctgtc ccggccataa gcgccaggat
     2761 aagagtttta ggtctgaagg ggcgtcgttt aataatacca gtttgtagtc agtagccagt
     2821 agtcagtagt tcactaagtt tatttaattg aatcacttga ctgtttgcta atacgggg
//
LOCUS       DAOQDO010000610         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28334_length_2877_cov_3.026931, whole genome shotgun sequence.
ACCESSION   DAOQDO010000610 DAOQDO010000000
VERSION     DAOQDO010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28334_length_2877_cov_3.026931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PKZ53_10640"
     CDS             complement(<1..1189)
                     /locus_tag="PKZ53_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNJ40936.1"
                     /translation="MAIVRPFRAVRPPVDKVELVASVPYDVVNTEEARALAAGNPYSL
                     LHVSRPEIDLPEETDIHSDEVYAKGLENLKQLEADLPLLTEETPHLYIYRQIMDGRAQ
                     TGVVGCCAVDQYDNDIIRKHERTRKDKEDDRTRHIITLRMQTGPVFLTYRKQDTINEL
                     VTRYCAETPLYDFVAPDKVRHTVWSVPDSDALADAFSQVPQLYIADGHHRAASASRTR
                     AHFRAENQFHTGTEEYNFFLTVMFPDEQLKILPYNRVVKDLAGRTPEQFLAAVKEVVE
                     VSTSPFPAPTQKWTFSMYLQGRWYGLYLDTDTIDKDDPIKSLDVSLLQDRILAPLLNI
                     EDPRTDKRIDFVGGIRGTDELERLVNEGKAAVAFSMYPTSMDELMRVSDANEVMPPKS
                     TWFE"
     gene            complement(1327..1995)
                     /locus_tag="PKZ53_10645"
     CDS             complement(1327..1995)
                     /locus_tag="PKZ53_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ40937.1"
                     /translation="MEPTIYETTDLSLSELSSLYVLGMLDATEQAQYEAAFLDHPAQA
                     RTELASAEAHLGYLAFAAPPCDPSPSLRSRLMDAVQAEVSQTSPPPKATEVPASFRKP
                     DFMPETVVVRSNELPWKPFLAPGSEARHLYTDAHKREITYLVRAQQGVSFPPHRHADC
                     EEFFMLKGELEVDGIAYAAGDYVRAAGGSVHGPNHALSDCMFLLKVSIDNQILSQEEY
                     QTLS"
     gene            complement(2007..2636)
                     /locus_tag="PKZ53_10650"
     CDS             complement(2007..2636)
                     /locus_tag="PKZ53_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ40938.1"
                     /translation="MPVQENHTAQGGFIVPSANDKAELRAKEQGFLELIRQIARGDQA
                     ALGQLYDTTHRQIFGFILRILGDPGAAEEVTLEVFMQIWRQAADYDLQRGTPTAWMMM
                     MARSRAIDRFRSGDQERRRAEALDTVAAVRSKERSPEESAAQAERRHIVQQALSSLPS
                     EQRQCIELAYYSGLSHSEIADKLNQPLGTVKTRIRLAMGRLRDALQVFE"
BASE COUNT          709 a          712 c          760 g          696 t
ORIGIN      
        1 gctcaaacca ggttgatttt ggcggcatga cttcgttggc atcagagact cgcatcaatt
       61 cgtccattga cgtcgggtac atcgaaaacg ccacggcggc tttcccttca ttgaccaggc
      121 gttccagttc gtctgtaccg cgaattccac ccacaaaatc aatgcgttta tccgtacgcg
      181 ggtcttcgat gttcagcaac ggtgccaaaa tgcgatcctg gagcaaactg acatcgaggg
      241 atttgatggg atcatctttg tcaatcgtgt cggtgtcgag atagagccca taccagcgac
      301 cttgcaaata catgctaaag gtccatttct gggttggtgc cgggaatgga ctggttgaaa
      361 cttcaaccac ttccttgacc gccgccagaa actgttctgg tgtccggccc gccagatctt
      421 tgacaacccg gttatagggc aggattttta actgttcatc cgggaacatg acggtgagaa
      481 agaagttgta ttcttccgtt ccggtatgga attgattttc agcccggaaa tgagcccgag
      541 tccggctggc actggcggct cggtgatgac catcggcaat gtagagttgt ggtacctgtg
      601 aaaaggcatc ggccagggca tcagagtcag gaaccgacca cactgtatgc cgcaccttat
      661 ctggggcgac aaaatcataa agcggcgttt cggcacagta ccgggtgacc agttcattga
      721 tggtatcttg tttgcggtag gtcaaaaaga ccgggccagt ttgcatgcgg agggtaatga
      781 tgtggcgcgt ccggtcatct tccttatctt tgcgtgtgcg ttcgtgtttc cgaatgatgt
      841 cgttatcata ctgatccacg gcacagcatc cgacgacgcc ggtttgagcc cgtccgtcca
      901 taatctgacg ataaatatag aggtgtggtg tttcttcagt cagcagcggg agatcagctt
      961 ccagttgctt gagattttcc agccctttgg catagacctc atcactatga atatcagtct
     1021 cttcagggag atcaatctca gggcgtgaaa cgtggagcag gctgtaggga ttcccggctg
     1081 ccagcgcacg cgcttcttcg gtatttacca catcatatgg aacgctggcg acaagctcca
     1141 ccttgtcaac tggaggccgc acagcacgaa atggacggac aatagccata aagttctcct
     1201 cagcaaatga agaatgaagg aataaaaatg tgtgttgaaa gcaatgctga acgatgactt
     1261 gggtccaggc actctctttg ggaggataag cggttaggaa tgcacagatt agggcaaacc
     1321 agacgattaa gaaagagtct ggtattcttc ttgagataag atctggttat caattgagac
     1381 tttgagtaaa aacatgcaat cgctcagagc atgatttggg ccatggacac ttccaccagc
     1441 ggctcgcaca taatctccgg cggcataggc gattccatca acttcaagct cacccttgag
     1501 cataaaaaat tcctcacaat cagcatggcg gtgcggagga aatgaaaccc cttgttgagc
     1561 tcgaaccaga taggtgatct cccgtttgtg ggcatcagtg tacagatgcc tggcctcgga
     1621 tcccggagca agaaagggtt tccagggcaa ttcgtttgaa cgaaccacca ctgtttctgg
     1681 cataaaatct ggttttcgga aagatgccgg tacttctgtc gccttcggag gtggcgaagt
     1741 ctgagagacc tcagcctgaa cggcatccat cagacgtgaa cgaagtgatg gtgatggatc
     1801 acagggcggg gcggcaaacg ccagataacc taagtgagct tcagcgctgg caagttcagt
     1861 ccgggcctga gccgggtgat caagaaacgc tgcttcatac tgtgcttgct ctgttgcgtc
     1921 gagcattccc agcacataga gcgaactcaa ttcgctcaaa gacagatcgg ttgtttcata
     1981 tattgtcggt tccacaagca caccccctat tcaaaaacct gcagtgcatc acgaaggcga
     2041 cccattgcca ggcgaattcg tgttttgact gtgccaagcg gctggttgag tttgtcggca
     2101 atttcgctgt gactcaggcc gctataataa gccagttcaa tacattgccg ttgctcagag
     2161 gggagtgagg aaagtgcctg ctggacaata tggcggcgtt cagcctgggc ggcggattct
     2221 tccgggctgc gttctttgga tctgacggcg gccactgtat ctaaggcttc ggctcttcgt
     2281 cgttcctggt cgcctgaccg gaagcgatca atggcccggc tccgagccat catcatcatc
     2341 caggcggttg gtgttccacg ttgcaggtca taatcggcgg cttgccgcca gatttgcata
     2401 aagacctcaa gggtcacttc ttcagccgct ccaggatctc ctaaaatccg caggataaac
     2461 ccgaaaattt ggcggtgtgt ggtgtcataa agttgaccaa gagcggcctg gtcgcctctg
     2521 gcaatctgac gaatcagttc cagaaatccc tgctctttgg cacgaagttc agctttgtca
     2581 ttcgctgagg gcacaataaa ccccccttgc gcggtgtgat tttcttgcac aggcattgag
     2641 aattaagaat gagatgcgaa cacaaaatga tgccacaaga aaaagaatgt gatgaattga
     2701 agtctaatga gcgagaacca accgtaatgg cccaagaccc ttgcaaagta gtatggattc
     2761 ccaggctcgt caagattgtc cgtcaccaac tcacttgaca tcctccccgc aataaattgc
     2821 aaggattccc ttgtgtgaca actcaatgcc gttactgaca caaaggaacg gggtgac
//
LOCUS       DAOQDO010000611         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28335_length_2877_cov_2.980865, whole genome shotgun sequence.
ACCESSION   DAOQDO010000611 DAOQDO010000000
VERSION     DAOQDO010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28335_length_2877_cov_2.980865"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1264
                     /gene="lpxD"
                     /locus_tag="PKZ53_10655"
     CDS             197..1264
                     /gene="lpxD"
                     /locus_tag="PKZ53_10655"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNJ40939.1"
                     /translation="MKLSEIASQLNCTIEGDPECEITGVAGLDEAGPTDLAFLSNPRY
                     TPKLQTTHAAALILNQTASVNWTKPILRAPDAYLAFAQVLGLFYQPPDLAPGIHPTAV
                     IAATAQLGQDVRIGAHVVIGDQALIGDRVTIHPNCTIYPGVKIGEDSYLHSNCVVREF
                     VEIGPRCRLQNQVTLGSDGFGYARQANGSWYKIVQSGLVILEADVEIGAGTQIDRASI
                     GETRIRRGAKIDNLVQIGHGSTVGEDTLLCAQVGLAGSTTVGNRVTLAGQVGVAGHLT
                     IGDGVLATAQSGIPNSVEAGKRVSGYPAIENRQWLKASAIFPKLPDLLKQLHQLQERV
                     ATLENQIQSGESSERVNETHD"
     gene            1381..>2877
                     /locus_tag="PKZ53_10660"
     CDS             1381..>2877
                     /locus_tag="PKZ53_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40940.1"
                     /translation="MPEPENRFPEEEQPKRKRTLAGELASKLAELGDEDISAAIDGLM
                     SPSGAYAAVTPQERLAHDRQTDAELKEMFSNIVLQFLEPVGYAIRQLTEIVPQAVTNP
                     SLMTTIRGTIDACTGSLKPLINAADTIKYHDIYDVLKAIERPLTDIQQGKRKTLTERD
                     AKNLNRDFTELTRLIQQSLGQEPQSLQSQQGSDVKERFRTASNYLISEMLPYYKDVSP
                     ADIQKIHAAGITRLNDLVMASAQEISVASSVSVEAAEHIKLCTFRALMNTQSPAQAVL
                     PPTRPATTTAPSHPPMAANLGSSYPPPAGPISAHLPPMEAALEAEAAPTLPEMPPIEA
                     LLGETSSIPTHFFEPAVNPGEQERLLATLEVIKKSVSTYSQSATNLNVELERTHRALL
                     SLRVERERMRSELEFHREELKQFFEQAGTETEENQDVLAVHKAMVASLRQVTEIIVFA
                     MKKIDIIYTQMEDSVRDVRDLETDLVELRRKRRSPRVGRSRQPNLEGDS"
BASE COUNT          747 a          799 c          704 g          627 t
ORIGIN      
        1 tggggcttct tcgaaagtat tgattcctaa gcactaagca ctaagcacta agcactaagc
       61 acaagcacta agcaccaggc acccatcgtc cttcgatttc attatttttg gctgatttca
      121 cggcacttgg cacaacctga accaggcgtc tggtcttttg agacgcgggt tcaggttcaa
      181 agtgccgctg ttttttatga agctgtctga aattgcatct caactcaact gcacaattga
      241 aggtgacccg gagtgtgaaa tcacaggcgt cgccgggctg gatgaagcgg ggccgacgga
      301 tttagcgttt ctttcgaatc cacgctacac tcccaaactg caaacaactc acgccgcagc
      361 tttaatttta aaccaaaccg cttcggtgaa ctggaccaaa cctatcctcc gggcaccgga
      421 tgcctatctg gcctttgccc aggtccttgg tctgttttac cagccacctg atctggcccc
      481 cggtattcac ccaaccgccg tcattgccgc caccgcccaa cttggacagg atgtccggat
      541 tggtgcccac gttgtgattg gcgaccaggc actgattggt gaccgggtga ccatccatcc
      601 aaattgcacg atttaccctg gcgtcaaaat tggtgaggac agctatctcc actcaaattg
      661 cgtggttcgg gaatttgttg aaatcggccc tcgctgccgg ctacaaaatc aggtaaccct
      721 cgggagcgac ggctttggct atgcccgcca ggcaaatgga agctggtaca aaatcgtcca
      781 gtcgggtctc gtgatccttg aagccgatgt tgaaatcggc gcggggactc aaattgaccg
      841 ggccagcatt ggcgaaaccc gaattcgacg gggtgccaag attgacaacc tggttcaaat
      901 cggccacggt tcaacggttg gggaagacac attactctgt gcccaggtgg ggctcgcagg
      961 cagtactacc gttggaaacc gggtcacgct ggcgggtcaa gtcggagtcg ctggccacct
     1021 gacgattggc gatggggtac tggccacggc ccaaagcggt attcccaact cggtcgaagc
     1081 tggaaaacgg gtctcaggct acccggccat tgagaaccga cagtggttaa aagcatctgc
     1141 catttttccc aaattgccag acctgctcaa acaacttcat caactccaag agcgggttgc
     1201 caccttagaa aatcagattc aatccggtga aagtagcgaa cgagtgaacg agacacacga
     1261 ctgaatttca atggttgaca gaaacatcaa ggcacttcac gacttcgcaa ccttgttctt
     1321 tcgctctttt gctctttcgc tcccttgctc atggtcagct accccaagga gtcttgcagt
     1381 atgcccgaac cagaaaatcg atttcccgaa gaagaacaac ccaaacggaa gcggacgctc
     1441 gccggggaat tagcttcaaa gctggcagaa cttggagatg aagatatctc agccgccatt
     1501 gacgggttaa tgtccccgtc tggcgcctat gcggcggtca caccacagga acggctggcg
     1561 catgatcgtc aaaccgatgc cgaactgaag gaaatgttta gcaatatcgt gcttcaattc
     1621 ttggagccag ttggctatgc cattcgtcag ttgaccgaaa ttgtccccca ggccgtcacc
     1681 aacccgagcc tcatgaccac gattcggggc accattgatg cctgtactgg ctcattaaaa
     1741 ccgcttatca atgcagcaga tacaattaaa taccacgata tttatgatgt tttgaaggca
     1801 attgaacgcc cgctgactga tattcaacaa ggcaaacgga aaaccctgac cgaacgagac
     1861 gccaaaaatt taaatcgcga ttttactgaa ctcacccgat tgattcagca gagcctcggc
     1921 caggaacccc aaagtctgca atcgcaacaa gggagcgacg tcaaagaacg gttccgaaca
     1981 gccagtaatt acttgatttc agagatgttg ccttactaca aagatgtcag cccagcagat
     2041 attcagaaaa tccatgcggc tggaattacc cgactcaatg acctggtgat ggcatccgcc
     2101 caggaaatct cggtagcttc aagtgtgtca gtggaagcgg ctgaacacat taaactctgt
     2161 acctttcggg cgttaatgaa tacccaatca ccggctcaag cggtactccc gccaacccgt
     2221 ccggccacca ccacggcgcc atcacatccc ccgatggctg ccaatcttgg atcatcgtac
     2281 ccacctccag ccggcccgat ttcggcccat cttcccccga tggaggcagc cctcgaagcc
     2341 gaagcagcac caaccttgcc agagatgccg ccaattgaag ccttgctggg cgaaaccagt
     2401 tcgattccaa cccatttttt tgaaccagca gtcaatcccg gcgaacagga acgattactg
     2461 gccacactcg aagttattaa aaagagtgtt tcaacctata gccagtcggc aacaaattta
     2521 aacgttgaac tcgaacgcac acatcgtgcc ttgctgagcc tgcgagttga gcgcgagcgg
     2581 atgcgcagtg aactcgaatt tcatcgcgaa gaactcaagc aattttttga acaggcgggc
     2641 accgaaacag aagaaaatca ggatgttctg gcagttcaca aagccatggt ggcctcactg
     2701 cgccaggtca ctgaaatcat tgtgtttgca atgaagaaaa ttgatattat ctacacccag
     2761 atggaagatt ccgtgcgaga tgtacgcgat ttggagaccg acctcgttga gctgcgccgg
     2821 aaacggcgaa gcccccgcgt gggacgttca cgtcaaccca acctggaagg cgatagt
//
LOCUS       DAOQDO010000612         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28340_length_2877_cov_2.410347, whole genome shotgun sequence.
ACCESSION   DAOQDO010000612 DAOQDO010000000
VERSION     DAOQDO010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28340_length_2877_cov_2.410347"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..849
                     /locus_tag="PKZ53_10665"
     CDS             169..849
                     /locus_tag="PKZ53_10665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ40941.1"
                     /translation="MSYPRVSVIVAVRNGERFLEQALQSIEKQTLEPFETLVIDGHST
                     DRTADIARSFSQVSYHLQAEKGISDAYNCGIQLARGELIAFLSHDDLWTPDKLETQAT
                     FMVDHPEVDFSLTHMKFFLEPGELPPPGFRESWLVGDQAGQIMETLMVRPKVFERTGL
                     FDTALSTGEDLDWFARATDMGMSKAVIPQVLLHKRIHSTNLSLTTTENNQNVLAALRR
                     SILRKRHG"
     gene            842..1549
                     /locus_tag="PKZ53_10670"
     CDS             842..1549
                     /locus_tag="PKZ53_10670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNJ40942.1"
                     /translation="MAELLSLPKPNPSLISVVIPVYNGELYLAEAIQSVLDQAYQPLE
                     IIVVDDGSTDRSVEIAAGFPHIQVVSQPKAGPGAARNTGVQTATGNWLAFLDADDVWT
                     RQKLIRQLAAFEQDPELDVVFGYVQQFVSPELGDEVRTRLKCPPNPMPGLHPGTMLIK
                     RKSFFQIGLLNSALVLGDFLDWHGRATDAGLKMLMLDEVVMCRRLHTTNLTLREKALQ
                     TDYVKMLKSTLDRRRAR"
     gene            1503..2771
                     /locus_tag="PKZ53_10675"
     CDS             1503..2771
                     /locus_tag="PKZ53_10675"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNJ40943.1"
                     /translation="MSKCSNQPLTVAVPDKILSDFSPTQNLIEAELYVFGNFERHNLI
                     MSHPYPSHLKINRPGAEQELLLQAALLNGEQALAAWKTWCSKVDLQALDTSSYRLLPL
                     LYHNLNRLQVKHPWLPIMRGVHRHTWVKNQTLLYEMGQLLTQFQDAGIEPLLLKGTAL
                     IVKYYDNFGLRPMDDFDVLVPRCQVNQAIEVLTKAGWEAGFRAPHAQSFRKGEELECD
                     LHWNILFENCRQAIDDPFRQMADQAYVNGVAVKVLNPTDQLLHVCVHGARWSYLPPLR
                     WLADAVVILNHSMIDWERLVQQAIRVGVVLPLRDALAYLKWLLGLEIPETVLSQLNQY
                     PVPLALHLDFRAWTIPADQRSASLTLWAYWREFVQWSYGANQSPVFWRFPLFLQHLWE
                     VDSIQKVPWMVVSGGLRRVGNRFLGRGLNS"
BASE COUNT          708 a          674 c          752 g          743 t
ORIGIN      
        1 aatgaagaat gaagaatgaa gaatgaagaa tgaagaatga agaatgaaga atgtggttag
       61 tacctggtgc ttggtactga gtgcttggga tccaatactt tcgaagaacc aagaaccaag
      121 aaccaagaac caatacaaaa attcataatt cacatctgga gtatccacat gagttatccg
      181 cgtgtgagcg tcattgtggc tgtcagaaat ggtgagcgat ttttggagca agcattgcaa
      241 agcatagaaa aacaaacgct tgaaccattt gagacgctgg tgattgatgg tcattcaact
      301 gaccggacgg ctgacattgc tcgttctttc tcccaggtca gctaccattt gcaggctgag
      361 aagggaatta gtgatgccta caattgcggg attcaactgg cccgagggga attgattgcc
      421 tttttgtcac acgacgacct gtggacacct gacaaactgg aaacccaggc cacgtttatg
      481 gttgatcatc cggaagtgga cttttcactc actcacatga aatttttcct ggagccaggt
      541 gagttgccac cacctggttt tcgcgagtcg tggctggtgg gcgaccaggc gggccagatt
      601 atggaaaccc tgatggttcg accaaaagtg tttgagcgca caggcctgtt tgacactgcg
      661 ctttcaaccg gagaagatct tgactggttt gcccgggcaa ccgatatggg catgtccaaa
      721 gcggtgattc ctcaggtgtt gctgcacaaa cggattcatt caaccaacct ttcgctcacg
      781 acgaccgaaa ataaccagaa tgtgcttgcg gcactccgcc gctcgattct tcgcaaacga
      841 catggctgaa cttttgtcac ttcccaaacc aaatccgtca ctgatcagtg tggtcattcc
      901 ggtctataac ggtgaacttt acctggctga agctatccaa agtgtgcttg atcaagcgta
      961 tcaaccgctt gaaatcattg tagttgacga tggttcaacc gaccgaagcg ttgaaattgc
     1021 ggctggcttt ccacacattc aggtggtatc tcaaccgaag gctgggccag gggcggctcg
     1081 caacaccggg gtccagaccg caaccggcaa ctggctggca tttctggatg cggatgatgt
     1141 gtggacccgt cagaaattga ttcggcaact ggctgcgttt gaacaagatc ctgagctgga
     1201 tgttgtcttt ggatatgttc agcagtttgt cagtccagaa ctcggcgatg aagtgagaac
     1261 acgattgaaa tgccctccga atccaatgcc agggttacac ccaggaacga tgttgatcaa
     1321 gcgcaagtca ttttttcaga tcgggctcct taattcggcg ctggtcctgg gagattttct
     1381 tgactggcac ggtcgggcga ccgatgccgg gctcaaaatg ttaatgcttg atgaggtcgt
     1441 catgtgccgt cggttgcaca ctaccaattt gactttgcgt gaaaaagcat tacaaaccga
     1501 ttatgtcaaa atgctcaaat caacccttga ccgtcgccgt gccagataag atcctttctg
     1561 atttctctcc aacacaaaat ctgatagaag ccgaactata tgtgtttggt aactttgaac
     1621 gccacaattt gattatgtcc catccgtacc catcacacct caaaatcaat cggcctggtg
     1681 ccgagcagga attgttactt caggccgcat tgctcaacgg tgaacaggct ctggctgcct
     1741 ggaaaacctg gtgttcaaag gttgatttgc aagcccttga caccagttcc tatcggctat
     1801 tgccattgct gtatcacaac ctgaaccggc tccaggtcaa acatccgtgg ttgccgatca
     1861 tgagaggagt tcatcgccat acctgggtta aaaatcaaac gttgttgtat gagatggggc
     1921 agctcctcac gcaatttcaa gacgcaggca tcgaaccgct tctgctgaaa ggaacggctt
     1981 taattgtaaa atattacgac aatttcggtt tgcgcccaat ggatgatttt gatgttttgg
     2041 tcccacgttg ccaggtcaat caggccatcg aagtgctcac caaagctggc tgggaggccg
     2101 ggtttcgggc accacacgct cagagttttc gcaagggcga agagttggag tgtgacttgc
     2161 actggaacat tttatttgaa aactgtcggc aagcgattga tgatccgttc agacagatgg
     2221 cagaccaggc gtatgtcaat ggtgtggcag taaaagtcct caacccaact gaccagcttt
     2281 tacatgtgtg tgttcatggc gcacgctgga gctatcttcc tcccttacgt tggttagctg
     2341 atgcggttgt gattctgaat cactcgatga ttgattggga gcgactggtt cagcaggcca
     2401 ttcgagttgg ggtggtcttg ccgttgcgtg atgcgttggc gtacttgaaa tggttactgg
     2461 gtctggagat tccagaaact gtgttgagcc aactcaatca gtacccggtt ccgctggctt
     2521 tacaccttga ttttcgagcc tggacaattc cggcggacca gcggagtgcc agcctcaccc
     2581 tctgggcgta ctggcgtgaa tttgtacagt ggtcctatgg tgccaatcaa tcaccagttt
     2641 tctggcgatt tccgctcttt ttgcagcatc tttgggaagt tgattcaatc caaaaagttc
     2701 cgtggatggt ggttagtggg ggacttcgac gggtggggaa ccggttcctg ggccgagggc
     2761 tgaacagtta gaaaagggcg gtgaagcaca acgcacaaaa tgccagtccc caacattttt
     2821 cagcctctcg atggtggttc cgcgttggtt aagtcaggaa ttctttgagc cgatcat
//
LOCUS       DAOQDO010000613         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28390_length_2874_cov_3.762327, whole genome shotgun sequence.
ACCESSION   DAOQDO010000613 DAOQDO010000000
VERSION     DAOQDO010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28390_length_2874_cov_3.762327"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2843)
                     /locus_tag="PKZ53_10680"
     CDS             complement(<1..2843)
                     /locus_tag="PKZ53_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ40944.1"
                     /translation="MHNLSALISGKNQPPVHLGGLHVVPAPIADQIAQFDLGLAVVET
                     RDTLLLSFEYNTDLFHCPTIERLAAHYQILLDSAITNPTQPIDQLNFISEAEYQSLKD
                     NWNLSRSVEFPAVAVHQLIELRVATQPEAIAVVFGTKSLTYHQLNTQADQVAWYLQQQ
                     GVGPATRVGICLERSLEMMIGLLAILKAGGAFVPLDPALPPERLAFMVADADLSIIVT
                     DQTSQPALPSNQAQLINLTNLDGSSFQCLPRAVPSLTPDHPAYVIYTSGSTGTPKGTV
                     IAHQAIVNLTCAAQQYLHLTPTDVVLQFASLSFDAALEEIFPTLASGAVLVLTKDPRF
                     LSFAEFHTLIETHAITVLDLPTAFWHHWVAELSRSDSNPINQSVRLVVIGGEAAQLDK
                     YVEWKQLTSGQNITWLNTYGPTEATVTALTYLPPDKEAFPTGSLPIGRPIANVSAVVL
                     DPWLQPVPVGVPGELCLGGLGLATGYLNRPELTAEKFIDWPAAPTPQLARLYRTGDLV
                     RLLADGNLQFLGRIDTQVKLRGFRIETGEIEAVLRSHPDVEQAVVILWDDPAVGQHLV
                     GYVATHTIGLQSQHLHEFLSSRLPAYMRPSALVVLTSLPLTTSGKVNRRALPAPDQLQ
                     INQHDPAARPLSELEVLLAGIWRDVLGNKPLTRHSNFFELGGHSLLATQIISRVRSFL
                     NLELPVRSVFEAPTLETFAQLVASIHRPADARGTPSVVPVSRVQKLPLSFAQQRLWFL
                     DQLEPDSPMYNLPGVLRLSGPLEVEALAQSLNEIISRHEALRTTFAQVDGQPVQVIAP
                     VGDQQLFITDLSSLSPPDQESQVQTLAIREAHQPFSLTTGPVIRFSVLRLAPDQHILL
                     VTIHHIAADGWSLSVFLTELHTLYQAFLHHQPSPLPPLTIQYPDFAVWQRQWLTDERL
                     RPQIDYWKQHLAGLESLNLPTDRPRPAVQSFRGT"
BASE COUNT          688 a          706 c          869 g          611 t
ORIGIN      
        1 gttccccgaa aactctggac ggctggtcgc ggtcggtcgg tggggaggtt gagcgattcg
       61 aggcccgcca ggtgttgttt ccagtaatca atctgaggtc gcagccgctc gtccgtcagc
      121 cactggcgtt gccagacggc aaagtcggga tattgaattg tgagcggcgg caggggggat
      181 ggctggtggt ggagaaaggc ttgatacaga gtgtggagtt cagtgaggaa cacactcaac
      241 gaccagccat cggctgcgat gtgatgaatg gtcaccagga gaatgtgctg gtctggtgcc
      301 agacgcagca cggaaaagcg aatcaccgga ccggtggtga gggagaacgg ctgatgagct
      361 tcccgaatcg caagggtctg aacctgggat tcctgatcag gtggtgacag ggaagaaaga
      421 tcagtgatga agagctgttg atcaccaacc ggcgcaatca cttgcactgg ctgaccatca
      481 acctgagcaa aggtggtgcg cagggcttcg tgacgggaaa tgatttcatt caaactctgg
      541 gccagggcct caacctcaag tggtccgctg agtcggagaa cgcccggcag attgtacatt
      601 gggctatccg gctcaagctg atcgagaaac cagagtcgtt gctgggcaaa cgaaagcgga
      661 agtttctgaa ctcgtgacac cggaaccact gacggggtgc ccctggcatc tgccggtcgg
      721 tgaatactgg ccacaagctg ggcgaaggtt tccaatgttg gtgcttcaaa cacggaccgg
      781 accggaagtt caagattgag aaagcttcgc acccgcgaaa tgatctgagt tgccagcagt
      841 gagtgtccgc ccagctcaaa gaagttactg tggcgggtca ggggtttatt tcccaataca
      901 tctcgccaga taccagccag taagacctcc aattccgaaa gaggcctggc ggctggatcg
      961 tgctgattga tctgcaactg gtcaggtgcc gggagcgccc ggcgattgac ttttccactg
     1021 gtggtcaggg gcaagctggt caagaccacc agggctgagg gacgcatgta tgctggcagt
     1081 cgtgagctta aaaactcgtg caggtgctga ctctggagtc caatggtgtg ggtggcaaca
     1141 tatccaacca gatgttgacc cactgctgga tcatcccaca ggatgaccac ggcctgttca
     1201 acatccgggt gcgaccgtaa caccgcctcg atttcacctg tttcaatgcg aaacccgcgc
     1261 agcttgacct gtgtgtcaat tcgaccgaga aactggaggt tcccatcggc caggagtcga
     1321 accaggtcac cagttcgata caagcgggca agctgtggcg tgggtgccgc gggccagtca
     1381 ataaatttct cggcagtcag ttctggacgg ttaagatagc cagtcgccag tcccaatccc
     1441 cccagacaca gctcccccgg cacacccact ggaacgggtt gcaaccaggg atcaaggact
     1501 acggctgaaa catttgcaat tggccgacct atcggcaggc tcccagttgg gaatgcttct
     1561 ttgtcaggtg ggagataggt caacgctgtg acagtggctt cggtgggacc ataggtattg
     1621 agccaggtga tattttgccc ggatgtcagt tgtttccact ccacatactt atcgagttgc
     1681 gctgcctcgc cgccaatcac caccagtcgg actgattgat tgatcggatt gctgtcggac
     1741 cggctcaact ccgctaccca atggtgccag aatgcagtgg gcaggtccaa caccgtgatt
     1801 gcgtgagttt caatcagcgt gtgaaattcg gcgaaggaca aaaacctggg atctttggtc
     1861 aacaccagca cggcaccgct ggcgagtgtg ggaaaaattt cttccaacgc cgcatcaaaa
     1921 ctgagcgatg caaactgtaa aacaacgtcg gttggggtca ggtgaaggta ctgttgggcg
     1981 gcacaggtga gattgacgat ggcctggtgt gcaatcacgg ttcccttggg ggtaccggtg
     2041 gatcccgatg tatagatgac atatgcggga tggtcaggag tgagggacgg aaccgcgcgc
     2101 ggaagacact ggaagctgga tccgtcaaga ttggtgagat tgatcaattg agcctgattg
     2161 gagggcaggg ctggttgaga tgtttggtcc gttacaatga ttgaaaggtc agcatccgct
     2221 accataaacg ccagccgctc aggtggaagc gccggatcca gtgggacaaa ggcaccacca
     2281 gctttgagaa ttgccaggag cccaatcatc atttccagcg aacgttcaag acaaattcca
     2341 acccgtgttg ctggcccaac tccctgttgt tgaagatacc aggcaacctg atcggcctgg
     2401 gtgttgagtt ggtgataggt gagtgacttg gttccaaaaa caacggcaat ggcctcaggt
     2461 tgagtggcaa cccggagttc gatcaactga tgaaccgcca cggctggaaa ctccactgag
     2521 cgagatagat tccaattatc cttcaagctc tgatattcag cctcagagat aaagttcagt
     2581 tgatcaattg gttgagttgg gtttgtgatg gctgagtcaa gcaagatctg gtagtgagcg
     2641 gccagtcgct caatggttgg gcagtgaaac agatccgtat tgtattcaaa cgacaacagc
     2701 agcgtatctc gggtttcaac gacggctaat ccaaggtcga attgcgcaat ctggtcggct
     2761 atcggagctg ggacaacgtg caaccccccc agatgaacgg ggggctggtt tttcccggaa
     2821 ataagtgctg acagattatg caatccctgg tcctggaggc ggtcaaagac aaat
//
LOCUS       DAOQDO010000614         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28395_length_2874_cov_2.759844, whole genome shotgun sequence.
ACCESSION   DAOQDO010000614 DAOQDO010000000
VERSION     DAOQDO010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28395_length_2874_cov_2.759844"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKZ53_10685"
     CDS             <1..775
                     /locus_tag="PKZ53_10685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ40945.1"
                     /translation="WLTAKERLHCPQAVVVTDFDVHAMWLCHHYQQFFVALDETRVHL
                     EKMGIPGEKITVSGIPIDPIFSQTKDKTAMRAKHGLEPQLTTILISAGGFGVGNMEHL
                     LTSLFELRHPVQIIAVCGKSAELKQRVEEVAAKRPTASQVRLKVLGFTTDMDELMSAS
                     DILLGKPGGLTTSEALAKGLVFVIVNPIPGQEERNSDHLLEEGVAIRCNNLPALAYKI
                     DRLLDNPERFAQMQQAALGMAHPDAARTIVTRLLELNQK"
     gene            complement(762..2435)
                     /locus_tag="PKZ53_10690"
     CDS             complement(762..2435)
                     /locus_tag="PKZ53_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40946.1"
                     /translation="MSEPIYPAATERELVLQREVEALRQEVVRLKQQNEGLQIALETT
                     AEHGDIIEDQLQNEILERERAELTLQSVLEVVSKKNTDLEIILQTTAEHGDMIENELR
                     RANQAKTMFLSNMSHELRTPLNAIIGFAQLLSRDPHQSLEHRRNIEIIVRSGEHLLNL
                     ISDILSLSKIEAGKLVLTSQTFDPRQMLKNLKDMMQGRARTKGIELTFEVSPSFPVLV
                     VGDEGRLRQILLNLLSNAIKFTQSGRVILRASWMEGRGYFEVEDTGQGIAPEELDSLF
                     IPFSQTQTGINLREGTGLGLAISRDLVELMGGAIQVRSQVGEGSTFWFEIDLPRSKDY
                     QIPLLSMRVLRLVPDQPVPKILVVDDNADHRRLLSQLLESAGFDVFEARNGKEAITFW
                     QTKRPQLLLMDIRMPKMNGDEATRTIRELEAQLNLPATPIVAISASVFDQDRALMAEV
                     GANGFLPKPFRESEVFQKLAELLDVNYEMVDTSADFDDEDILTPERYQQLSEEWRLQF
                     ESAVTIGDIVEVLGLVEEIKPLDPELAVALGRAASAVDFHLLERLFTSD"
     gene            complement(2432..2776)
                     /locus_tag="PKZ53_10695"
     CDS             complement(2432..2776)
                     /locus_tag="PKZ53_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010874259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40947.1"
                     /translation="MELKNNDYQVWFDPALNTVFCKGFLRLDGVDGYQEIMDLLLFGV
                     TAAHPKVTVDLSQLQFLNSSGISMLSMFVIKVREKGDLQLIVRGSEKILWQTKSLRNL
                     KRLLPAMTLEFI"
BASE COUNT          708 a          753 c          699 g          714 t
ORIGIN      
        1 gtggctgaca gccaaagaac gtctccactg cccgcaggcg gtggtggtga cagattttga
       61 tgtccatgcc atgtggttat gccaccacta tcagcagttt tttgtcgcgc tggacgaaac
      121 acgcgtccat ttggaaaaaa tgggaattcc aggtgagaaa atcacggtgt ctgggattcc
      181 aattgaccca atcttctcac aaaccaaaga caaaaccgcg atgcgggcca aacacgggtt
      241 ggagccacag ttgaccacga ttctgatttc ggcgggtggg tttggtgttg gcaatatgga
      301 acacctgctc acatcactct ttgaactccg ccatccggtg cagattattg ccgtttgtgg
      361 aaaaagtgcg gaactcaaac aacgggttga agaagtagcc gccaaacggc ccaccgcaag
      421 tcaggttcgg ctcaaagtgc ttggatttac caccgacatg gatgaattga tgtcagcttc
      481 ggacatcttg ctgggcaaac ccggtgggct gaccacgtcc gaagcgcttg ccaaagggct
      541 ggtatttgtc atcgtcaatc cgattcctgg tcaggaagag cgcaactctg atcatttgct
      601 tgaagaaggc gtcgccattc ggtgcaataa cctccctgcc ctggcttaca aaattgaccg
      661 gctactcgac aaccccgaac gctttgctca aatgcagcag gcggctctgg gtatggctca
      721 tccagatgcg gcccgaacga ttgtcacccg gttgctcgaa cttaatcaga agtaaacaac
      781 cgctccagaa gatgaaaatc aaccgcgctg gcggcccgcc ccagggcgac cgccagctct
      841 ggatccaggg gtttgatttc ttcgaccagg ccgagaactt caacaatgtc accgatggtc
      901 acggctgatt caaactgaag tcgccactcc tctgataact gctgatagcg ctctggggtg
      961 aggatgtctt catcatcaaa atcagcgctg gtatcaacca tttcatagtt gacatccagc
     1021 aattcagcca gtttttgaaa gacttcgctt tctcgaaacg gttttggcag aaatccattg
     1081 gcgccgacct ctgccatcag ggctcgatcc tggtcaaaca cactggctga aattgccaca
     1141 atcggggtgg cgggcagatt gagctgggct tcaagttcgc gaatagtacg ggtggcctca
     1201 tcgccattca ttttcggcat tcgaatgtcc atcagcaaca actgcggacg tttggtttgc
     1261 cagaaggtaa tcgcttcttt tccgtttcgg gcttcaaaca cgtcaaaccc cgcagattca
     1321 agcaactggc tcaacagccg ccggtggtcg gcattgtcat caaccaccag aatttttgga
     1381 actggctgat ctggcaccaa ccgcagcaca cgcattgaca gaagcggaat ctgatagtcc
     1441 tttgaccggg gaagatcaat ttcaaaccag aaagtcgaac cttctccgac ctgacttcga
     1501 acctggatcg ccccccccat caattcaacc agatcgcgac tgatggccag accgagtccg
     1561 gtgccctcgc gcagattgat tccggtttgg gtttgtgaaa acgggataaa taatgagtca
     1621 agttcttcag gagcaatgcc ttgaccggtg tcttcaactt caaaatagcc acggccttcc
     1681 atccaggaag cccgtagaat gacccgaccc gattgggtaa atttgatggc attgctcaac
     1741 aggttgagca aaatctggcg gagtcggcct tcatccccca caaccagaac tggaaaagaa
     1801 ggcgacactt caaaggtcag ctcaatgcct ttggttcgag cccgcccctg catcatgtct
     1861 tttaagtttt tcagcatctg gcgcggatca aaggtctgac tggtcaatac cagctttccg
     1921 gcttcgattt tggaaagcga taaaatatcg ctgatcaaat tcagcaggtg ctccccactt
     1981 cgcacaatga tctcaatatt tcgccggtgt tccagggatt gatgtgggtc acgactcagg
     2041 agctgggcaa acccaatgat tgcattaagc ggcgtgcgca actcgtggct catattcgac
     2101 aaaaacatcg ttttggcctg attggcacgg cgcagttcat tttcaatcat atcgccatgt
     2161 tcagcggtgg tttgcagaat gatttccagg tcggtatttt ttttggaaac gacttcgagc
     2221 acagattgca gcgtgagttc cgcgcgttcg cgttcgagaa tttcattctg gagctgatct
     2281 tcaataatgt cgccgtgttc agccgtggtt tcgagcgcaa tttgcaaccc ttcgttttgc
     2341 tgtttcaagc gcacgacttc ctggcgcaac gcctcaacct cgcgttgcaa taccagttca
     2401 cgttccgtgg ctgctggata aatcggctcg ctcatataaa ttccagtgtc atggctggta
     2461 gcaaccgttt gaggtttcgc agtgatttgg tttgccacaa aattttttcc gacccccgga
     2521 caataagctg taaatctcct ttttcacgaa ctttgatcac aaacatagat aacatgctaa
     2581 tgccggagct gtttaaaaat tgaagctggc tgaggtcaac cgttactttt ggatgggctg
     2641 ccgtgacgcc aaacagcagc aaatccataa tttcctgata cccgtccacg ccatccagcc
     2701 gtaaaaaccc tttacaaaac acggtgttga gcgccgggtc aaaccaaacc tggtaatcat
     2761 tgttttttaa ttccaacgtt gacatgtgtg gtgattcctt gtcttcaata aacctggacc
     2821 aggtgaagat ggcattcctg atcaagttct gcaattgatg taccagccag gaga
//
LOCUS       DAOQDO010000615         2873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28414_length_2873_cov_2.473385, whole genome shotgun sequence.
ACCESSION   DAOQDO010000615 DAOQDO010000000
VERSION     DAOQDO010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28414_length_2873_cov_2.473385"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKZ53_10700"
     CDS             complement(<1..813)
                     /locus_tag="PKZ53_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNJ40948.1"
                     /translation="MKAWEIRDNFGLENLKLADRAELQPGPGQVLVRVRAVSLNYRDL
                     MVSKGWYNPKQKLPLVPCSDGAGEVVAIGEGVKSVKPGDRVMSCFFQRWQNGELSDVD
                     ARSTLGSPADGMLREFAVLEEFGVIPTPSFLTDVEASTLPCAALTAWQALVTLGKIKA
                     GDTVLVQGTGGVSIFALQFAVKSGARVIATSSSDEKLQRAIELGASDGINYKTTPNWE
                     KRVRELTGGIGVDHVVEVGGAGTLSKSVIAVRRSGTVSLIGVLSGPGDFNPLP"
     gene            1057..1746
                     /locus_tag="PKZ53_10705"
     CDS             1057..1746
                     /locus_tag="PKZ53_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40949.1"
                     /translation="MKPLCRRGLQVTGQWMLLIVIGVWCVGVQDPVWAQKKQKGGPKP
                     KPAASKPTTSKPVASKPVTPQPPKDIKLMTDAEADKEMDRIDKEAEKKVKLIEADYDR
                     RIAKAEKIRDEEAREQAIADLEVEQEDKINQIEEDADIRILEIRTRKLNAEQKAQVYK
                     IKADYDAKIQTLEDEYDALIQEAEETEEEETVDDKIAQLEEELEAKIEDLEDEMDQKI
                     DEFIEKALKTK"
     gene            1938..>2873
                     /locus_tag="PKZ53_10710"
     CDS             1938..>2873
                     /locus_tag="PKZ53_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HNJ40950.1"
                     /translation="MSKLSSQPLPEPIWIEAEGGYWLALDGSKLMARNPKGQILQSLP
                     KQVKDLPLTEQLRNVASWLARHQAEGVEQLEQWMLRSLPIPTSLVIHLWDDSAWNQSF
                     KNTVIVPVDASSRAEFDQAGLLKAVDPVRGLGVVGLDGETHWLFPEQIIIPHPVHLLD
                     LQDWRELCVELGEKQALQQLFREIWIKPDVVQPDPFRFHGFANAEFEQLNHAFSRCRS
                     LGIRIRQGFASQTVWDRGTPVEVRFWIGEGYPENPTSTGYLGWCDNRGRLMPVTSVGS
                     VAFSEGCRLAQTIVSTAKQGVPEPPATAHSKLDIAS"
BASE COUNT          760 a          763 c          700 g          650 t
ORIGIN      
        1 cggaagcggg ttaaaatctc ctggcccaga aagtacacca atcaaactca ccgtaccgct
       61 ccggcggacg gcaatcactg atttggaaag ggttccggca ccgccgactt caaccacgtg
      121 atcaactccg ataccaccgg ttaattcacg cacccgtttt tcccagttcg gagtggtttt
      181 atagttgatg ccatctgaag cgcccagttc aatcgcccgc tgcaattttt catcgctgct
      241 cgacgtggca atcactctgg cgcctgattt caccgcaaac tgaagcgcaa aaattgaaac
      301 gcctcccgtt ccctgaacca gcaccgtgtc tccggctttg atttttccga gtgtgaccag
      361 cgcctgccag gcagtcagtg cggcacaggg caaggtagac gcttcaacat ccgtcaaaaa
      421 gctgggggtt ggaatcacgc caaattcttc caacactgca aattcccgca gcattccatc
      481 tgcgggagaa ccaagcgttg agcgggcgtc cacatcactg agttccccgt tttgccagcg
      541 ctgaaaaaaa caactcatca cccggtcacc gggcttgacg cttttgacgc cttcgccaat
      601 tgccaccact tcaccggcac catctgaaca aggcaccagc ggaagctttt gtttcgggtt
      661 gtaccagcct ttggaaacca tcaaatcccg atagttgagt gaaacggccc gaactcgcac
      721 caaaacctgc ccagggccgg gttgtaactc agcccggtcg gccagtttca ggttttcaag
      781 tccaaaatta tctcgaattt cccacgcttt cattgctcct ccttcagtat ttggagggcg
      841 gcagtcagcc gccgctttaa aatgcaacgg gtcaccgctg cgccttaact ctaccctaaa
      901 accatttcat cgtgcatctt cacaccgaaa ttcgcatcta tgtgcctgcc cctcagtttc
      961 tttccgcaga gttcttgcaa agctgaacct gaactattag aaagcagttt gctttcatct
     1021 ttatttttcc gtgtgtcgaa ccaaaaagga gccagtatga aaccgttgtg tcgccggggt
     1081 ttgcaagtga ccggccagtg gatgttgttg attgtaattg gagtatggtg tgttggtgtt
     1141 caggacccgg tgtgggccca aaaaaagcaa aaaggaggcc ctaaacccaa accggcggct
     1201 tccaagccca caacctctaa accagtcgct tccaagccgg tcacgcctca gccacccaag
     1261 gacattaaac tcatgaccga tgctgaagct gacaaggaaa tggatcggat tgataaagaa
     1321 gccgaaaaga aggtgaaact gatcgaggcg gattatgaca ggcggattgc caaagctgaa
     1381 aaaatccgcg atgaagaagc ccgcgaacag gcaattgcgg accttgaagt tgaacaggaa
     1441 gacaagatta accagattga agaagatgcg gatattcgaa ttttagaaat tcgaacccgt
     1501 aagctcaacg ctgagcaaaa agcccaggtc tataaaatca aagctgacta tgatgccaaa
     1561 atccagaccc tcgaagatga atatgatgcg ttgattcagg aagccgaaga gaccgaagag
     1621 gaagaaacgg ttgacgataa aatcgcccag cttgaggaag aactcgaagc caaaattgaa
     1681 gacctcgaag atgaaatgga tcaaaagatt gacgaattca ttgaaaaagc gttgaaaacc
     1741 aaatagaacc agccagtcgt cagtcgttca ccaggttggt ttggtgaaat cacatcacga
     1801 gctggccctt ctttttttcg caatcctgcg ttcgaaatca aaaaagttca gagtatcggc
     1861 ttgagacgag aaacaacaaa acctctcgcc agaaggcggt atcctgaact tttttcatgt
     1921 ctgaggaacc tgatcgcatg tcaaaattgt catcccaacc acttcccgag cccatctgga
     1981 tagaagccga aggcgggtac tggcttgctt tggacggatc gaaactgatg gcgcgcaacc
     2041 ccaaaggtca gattctgcaa tcgttaccca aacaggtaaa agaccttccg ttgactgagc
     2101 aactgcgcaa cgtcgcaagc tggctggcac gccaccaggc agaaggcgtt gaacaactcg
     2161 aacaatggat gctgcggtcg cttccgattc cgacatcact ggtaatccac ctctgggacg
     2221 attcagcctg gaatcagtcc tttaaaaata cggtgattgt tccggtggat gcctcgtcca
     2281 gagctgaatt tgatcaggcg ggattactta aagccgttga tccagttcgt gggctcggtg
     2341 tggttgggct tgacggagaa acccactggt tatttccaga acaaatcata attccacatc
     2401 cggttcacct gctggacctc caggattggc gtgaattgtg tgttgaactc ggcgaaaaac
     2461 aggcccttca gcagcttttt cgggaaatct ggatcaaacc agatgtggta caaccagatc
     2521 catttcggtt ccacggattt gccaatgctg aatttgaaca gctcaaccac gccttctccc
     2581 gctgtcgttc gcttggaatt cggattcgcc agggatttgc cagccaaacg gtgtgggatc
     2641 gcggaacccc ggttgaggtc aggttttgga ttggtgaagg ataccccgaa aacccaacct
     2701 ccactggcta tctgggctgg tgtgataacc ggggccggtt gatgccggtt acatccgtcg
     2761 gctcagtcgc tttttccgaa ggctgccgcc tggctcagac gattgtttca accgccaaac
     2821 agggtgtccc cgaaccacca gccactgcgc attcaaagct ggatattgcg tcg
//
LOCUS       DAOQDO010000616         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28430_length_2872_cov_2.782748, whole genome shotgun sequence.
ACCESSION   DAOQDO010000616 DAOQDO010000000
VERSION     DAOQDO010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28430_length_2872_cov_2.782748"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PKZ53_10715"
     CDS             <1..1276
                     /locus_tag="PKZ53_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNJ40951.1"
                     /translation="SVLEKKFVDTLQEFVHRSQGTWDKTIVRGRPGFRFTLPPSSRLW
                     ELELQPTLGSEQGVLITSKPDFMLRCDDESIKPIAIFTDGFQFHCHPHNRLADDMRKR
                     RAILESDRYQVWNLTWNDLEPAGAGQIMVAGPQLLAIMQKYIAGNPHRHKFPGLEQAF
                     GNGWQQLKAFLMKPDPKIWRNLAQSLVFLPLLSLASAKTVGITPLRGALETWKTGASM
                     PPVPSEQDSEWVCFHRITTTDDFVGCIAVPDALNNNQQKALMLGRLGDTEPERTDGSF
                     TDRWRRYLASLNLFQFCEQFRFWATSEIADCTAPEIVLTSTSLSFAPEWASVCDSVIP
                     SLRSLVQELAEADLPPAALPLVEFFNDEINDDAFAELAWPAGTVRIAVLAGEQTGFAT
                     RWQSLGWKVFTPEDVQAGRTGELIQFVREAHT"
     gene            1448..>2872
                     /locus_tag="PKZ53_10720"
     CDS             1448..>2872
                     /locus_tag="PKZ53_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNJ40952.1"
                     /translation="MAKLMVHRNILQKFSKLPLKVQKRIPELIDEFQKDPFSESIGMH
                     PVSESMLDSKVRGIKKLPDGYRAIVIAPERGDTYLLIYIDKHDEAYQWARNKRFEVHE
                     MTGVFQIFDAEELQSATTVTPSPQTGDYPLSRLSDDELFQAGVPKPLIPAVKSIQSDE
                     ALEALGQYLPPDCRDVLFGLAAGMALDDALAEMLGAAPRDTTTPAQGPGDFTNIEAAP
                     NFDLVLVEGHEHLKAILQGSIEEWRIFLHPYQRKLVEMNTTGPICITGSAGTGKTVAL
                     MHRAVNLARQLKDSQARVLVTTYTTNLSVTIKQHLQRLSPEAAPRIEVTNLHALARTI
                     CARAGWRGRVAEDEDMDQIWEEIWVKEKGDLPLSKEEMRKEYNQIVDPNGIEDEETYL
                     TTVRTGRPRLSRDQRKKVWSVFRLLQSGLKKRNLLTFEGAVHQARLAIEQGKSTRYAH
                     VLVDEIQDFGLEALRLIRAMSPITD"
BASE COUNT          738 a          742 c          735 g          657 t
ORIGIN      
        1 gagtgtactc gaaaagaaat ttgttgatac gttgcaggaa tttgtccacc ggagccaggg
       61 cacctgggat aaaacaattg ttcgcgggag accagggttt cgctttacac ttcctccttc
      121 cagccggtta tgggaattgg aactccagcc aacgctggga tcggaacagg gtgtcctgat
      181 aacctcaaaa ccagatttca tgctccggtg cgatgatgaa tccatcaagc caatcgcaat
      241 ttttacagat gggtttcaat tccattgcca tccccataac cgattggctg atgatatgcg
      301 caaacggcgt gcgatcctgg aaagtgaccg ctatcaggtt tggaatttga cctggaatga
      361 ccttgaacca gccggagccg gtcagataat ggtggctggg cctcaactcc tggcgataat
      421 gcagaaatat atcgccggca acccgcaccg ccataagttt cctggattgg aacaggcgtt
      481 tggcaacggc tggcagcaac tcaaggcatt tttgatgaaa cccgatccca agatctggag
      541 aaatttggcc caatcgctgg tgtttcttcc actcttgtct ctggccagtg ccaaaaccgt
      601 aggcatcaca ccgctccgtg gagcacttga aacctggaaa actggggcct caatgccacc
      661 agtgccgtcc gagcaggatt cagagtgggt ctgttttcat cgaataacca ccaccgatga
      721 ctttgtgggg tgtattgctg ttccagatgc actcaacaac aaccagcaaa aggcactgat
      781 gctgggaagg ttgggggata ccgaacctga acgtaccgat ggaagtttca ccgaccgctg
      841 gcggcgatat ttagccagtc tcaatctatt tcagttttgt gaacaatttc gattttgggc
      901 cacctccgaa attgctgact gcacagcgcc agagattgtg cttacctcaa cttcgctgag
      961 tttcgcgccg gaatgggcct ccgtttgtga cagcgtgatt ccaagccttc ggtctctggt
     1021 acaggaactg gctgaggccg atttgcctcc agccgcgttg ccgctggttg aatttttcaa
     1081 tgacgaaatc aatgatgatg cctttgccga gctggcctgg cccgctggta cggttcggat
     1141 cgccgttctt gccggagagc aaaccgggtt tgccactcgc tggcaatcac ttgggtggaa
     1201 agtctttacg cccgaggatg tgcaggcagg caggacaggc gagctgattc aattcgtccg
     1261 agaagctcac acatagtgat cagaacgaca atcaacaaca ggctatacca ttttgcaagg
     1321 aaaccttcgt attacgggaa agctaaatta ttggaagaat tggatttccc tgaaccctga
     1381 acccgatacc ctgaacccca aatggtatta tcactggtga aatgtactca acttgacgag
     1441 gaaaattatg gcgaaactga tggttcaccg gaatattttg cagaaatttt caaagctccc
     1501 gttaaaagtt cagaaacgaa ttccagagct gattgatgag tttcaaaagg accccttttc
     1561 ggaatccatt gggatgcacc cggtctcgga atccatgctc gattcaaaag tgagaggaat
     1621 taaaaagctc cccgatgggt atcgggcaat tgtgattgct ccagaacgcg gtgataccta
     1681 tctgttgatc tatatagaca aacacgacga ggcttaccaa tgggcacgca ataaacggtt
     1741 tgaagttcat gaaatgaccg gtgtatttca gatttttgat gcggaagaac tccaatccgc
     1801 aaccactgtg acaccctcgc cccaaactgg tgactatcca ctttcccggt tgtctgacga
     1861 cgaattgttt caggccggag tccctaaacc actgattcca gcagtcaaat ccatccagtc
     1921 agatgaagcc ctcgaagccc ttggtcagta tttaccaccc gattgccgtg atgtgctgtt
     1981 tggtctggcg gcgggaatgg cgctggatga tgcactggcg gaaatgctgg gggcggcccc
     2041 cagagacacg accacaccgg cgcaaggccc aggcgacttc actaatatcg aggcggcacc
     2101 aaactttgat ttagttctgg tcgaaggcca cgaacatctg aaagccattc tgcagggttc
     2161 gattgaagaa tggcggattt tcctccatcc ctaccagcgc aaactggttg aaatgaacac
     2221 cacgggacca atctgcatca ctggttcggc tggtaccggc aaaacggttg ccctgatgca
     2281 ccgggcagtc aatctggctc gccagctcaa ggactcacag gcacgggtgc tggtcaccac
     2341 ttacaccacc aatttatccg tcaccatcaa gcaacatttg cagcggctgt ctccagaggc
     2401 cgcaccccgg atcgaggtca ccaatttaca cgcactggcc cggaccatct gtgcccgggc
     2461 cggatggcgt ggacgggtgg ccgaagacga agatatggat caaatctggg aagaaatctg
     2521 ggtaaaggaa aaaggagact tgcctttgtc caaagaagaa atgagaaaag aatacaacca
     2581 gattgtggat ccaaacggca ttgaagacga agagacctat ctgaccaccg ttcgtactgg
     2641 tcgtccccgt ctgagccgtg accagcgcaa gaaagtatgg tcggtttttc gcctgttgca
     2701 aagcggcttg aaaaaacgca atttactcac atttgaaggt gccgtgcatc aggcccggct
     2761 cgccatcgaa caaggcaaat ccaccaggta tgctcacgta ctggtagacg aaatccagga
     2821 tttcggcctc gaagccctcc ggcttatccg ggccatgagt cccatcacag ac
//
LOCUS       DAOQDO010000617         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28432_length_2872_cov_2.220447, whole genome shotgun sequence.
ACCESSION   DAOQDO010000617 DAOQDO010000000
VERSION     DAOQDO010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28432_length_2872_cov_2.220447"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKZ53_10725"
     CDS             <1..197
                     /locus_tag="PKZ53_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40953.1"
                     /translation="LCESEGVIQARSVSTSVRINELLRQKTPRRRCRECAQVLPIGHR
                     YRLCEPCFQAQREDWYFDDY"
     gene            422..>2872
                     /locus_tag="PKZ53_10730"
     CDS             422..>2872
                     /locus_tag="PKZ53_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ40954.1"
                     /translation="MDSFCLSRVRIFACIFFLLLVIAAAAGLEPTSSLADGLTIKIRQ
                     RHPVVNEGNQIQLTVMDANGQSLDDVRWGSGSPDIARIDPQTGVVSGVRQGFATITAF
                     HGNQSTSVFVVVARVKKGKAAAIPGDTKIDQGGQLYISNPLKNIILKASSALVSEPQV
                     FAGQQGVPGFRNGNPLQSLFAGPTALAIDNQVSGGIFIADTLNHSLRRISFTNQVETV
                     LGTGSPGITPFGSNRMAAFEAVQLHSPRGVASESNGNVFVADTENHALYFVNLATRTV
                     QLLAGTPGEPGKGDGPGQTARFSRPAGLALSRDGRVLAVADQDNNCVRLLELIKGSNA
                     QMLCQVSTVSATGPQPRATSNPEPDEFEFNHPQSVSFDSSGNIIVVDESSVYVIARPL
                     DPTRFVIQLAQLESYSKAVSATVRGTETFVLDAEAVNPSEAITVVTVGAPEITGLSQE
                     SVRIEGGAEIILTGRNFAPETLVFIGDTQVENFQVESATQIRLIVPRQSSHGDRTLTV
                     QTRGGVAQAKLTVLPKPFAELVDGEITTLAGGVPYVGDGGLATAPGVALNPFQTIVDG
                     AGNFYIADGFSNRIRRIAQDSGIITTVAGNGNYGFTGDGGPATTAGLAGPTGIAFDRQ
                     GNLHILDSGNLRIRRVDATTGVITTVAGDGRFNVGDDNGDGHVATQASFRKPVAIAFD
                     SSDNLYIADALDYRVRRVDAQTRVITTVAGSGELPSSGEPNGDGGPAIQAKFVEIKGI
                     AVDGQGNLLITDGFAHRVRQVDFKTGIITTIAGVGTRNGIPVFDGDGGPATNANLNAP
                     GAVAIDSFGNILIADTRNR"
BASE COUNT          671 a          704 c          790 g          707 t
ORIGIN      
        1 agttgtgcga atcagaaggg gtcatccagg ctcggagtgt cagtaccagt gtgcgaatca
       61 acgagttgct tcggcaaaag accccacgtc ggcggtgtcg tgaatgtgcc caggtgctgc
      121 cgattggcca ccgctatcgg ctgtgtgagc cctgttttca ggcgcaacgg gaagactggt
      181 actttgatga ctattaattt gatttgggcc ggctgaagat ctgaagcgcc gaaattgaat
      241 gggcactttt cactggttga cccatcgccc aaaaataatt ttttttcgtg agacaatttt
      301 tgacttccca ctccgcgttt aaccgtagaa gaccaaatac cctctcaatg gtattcttcc
      361 tgtctttctt gccttacccg ttcagacgaa gccagccata atttttttca gaggtccttg
      421 tatggattct ttttgtcttt ccagggtcag gatttttgcg tgtattttct ttcttttgtt
      481 ggtaatcgcc gccgccgcag gattagaacc gacgtcttct ctggcggatg gtctaaccat
      541 caaaatcagg caacgacatc cggtcgtcaa tgaagggaac cagattcaac tcacggttat
      601 ggatgcaaat gggcaatcac ttgatgatgt caggtggggg agcggaagcc cggacattgc
      661 ccgcattgac ccacaaaccg gggttgtcag cggagtccgt cagggctttg ccaccattac
      721 ggcgttccac ggcaaccaga gtacttccgt ttttgtagtc gtggcgcgag tcaagaaagg
      781 caaagcggcg gcgattcctg gtgataccaa aattgaccag ggcgggcagt tatatatcag
      841 caatccattg aaaaacatca tcctcaaagc ctcctctgcg ctggtttccg agccgcaggt
      901 ttttgctggt cagcaggggg tgccaggatt tcgcaatgga aacccgctgc aatcattgtt
      961 tgctggaccc acggcgctgg caattgacaa ccaggtatca ggcggcattt ttattgcgga
     1021 cacgctcaat cacagcctcc gccggatttc ttttaccaac caggttgaaa ctgtgcttgg
     1081 aactggctca ccaggaatta ccccgtttgg gtcgaaccgc atggctgcct ttgaagccgt
     1141 gcaattgcat agtcccaggg gcgtagccag cgaaagtaat ggaaatgtat ttgtggctga
     1201 tacagaaaat cacgcgctgt actttgtgaa tcttgccaca cgcacggttc agttgctggc
     1261 cggcaccccc ggagaaccag gaaaaggcga tggccctggg caaacggcgc gattctcgcg
     1321 cccagccgga ctggctttga gccgcgatgg acgagtgctg gcggtcgctg accaggataa
     1381 caattgcgtc cgattgcttg aattgatcaa aggatcaaat gcccagatgt tgtgtcaggt
     1441 aagcactgtg agcgccaccg gacctcaacc acgggcaact tcaaatccgg aacccgacga
     1501 atttgagttc aatcatccac agtcagtgag ttttgatagc tccggaaata ttattgtggt
     1561 tgatgaatca agcgtctatg tgattgctcg acctctggat ccgacccggt ttgtgattca
     1621 actggcacaa cttgaaagct actcaaaagc agtcagcgcg acagttcggg gaaccgaaac
     1681 ctttgtcctg gatgccgaag ccgtcaatcc ttccgaagcc atcacggtag tcacggttgg
     1741 ggcgccggaa atcactggct tgagccagga aagtgtccgc atcgagggag gggcggaaat
     1801 cattttgacc ggaaggaatt ttgccccaga aacgcttgtc tttattgggg atacccaggt
     1861 tgagaatttt caggttgaaa gtgcaaccca aattagactg atcgttccca ggcaatcttc
     1921 ccatggtgat cgaaccttga ctgtacaaac ccgaggtggt gttgcccagg ctaaattgac
     1981 ggtgcttcca aagccatttg ccgaattggt tgatggtgag atcacaactc tggcaggcgg
     2041 agtgccatac gttggggacg gcggtctggc cacagcgcca ggggtggcct taaatccatt
     2101 tcaaaccatt gtggacgggg ctggcaattt ttacattgcc gatgggtttt ccaatcgaat
     2161 ccggcggatc gcccaggatt caggaatcat caccaccgtg gccggaaatg ggaactatgg
     2221 atttactgga gatggcggcc cggccacaac ggctggattg gcaggcccaa ctggcattgc
     2281 ttttgaccgt cagggcaatt tgcatatcct ggattctggc aatctaagga ttcgccgggt
     2341 tgatgccacg accggcgtta tcaccactgt ggccggagat ggcaggttca atgtgggtga
     2401 tgataatggt gatgggcatg tagcaaccca ggcctcattc agaaagccag tggcgattgc
     2461 gttcgactca tctgacaatc tctatattgc cgatgctttg gattaccggg tccggcgggt
     2521 tgatgcacaa accagagtga ttactacggt tgccggttct ggtgaattgc catccagcgg
     2581 ggaaccaaat ggtgacgggg gacctgccat ccaggcaaaa tttgttgaaa tcaaaggaat
     2641 tgcagttgat gggcaaggaa atctgctgat tacagatggt tttgcccacc gggttcgcca
     2701 ggtagatttc aaaaccggta tcatcaccac cattgccgga gttggaactc gcaatggcat
     2761 tccggttttt gatggtgatg gtggcccggc gacaaatgcc aatttgaacg cccccggtgc
     2821 agtggcaatt gatagttttg gaaatatcct catcgctgat actcggaacc gc
//
LOCUS       DAOQDO010000618         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28458_length_2870_cov_4.387211, whole genome shotgun sequence.
ACCESSION   DAOQDO010000618 DAOQDO010000000
VERSION     DAOQDO010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28458_length_2870_cov_4.387211"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1135
                     /gene="miaA"
                     /locus_tag="PKZ53_10735"
     CDS             164..1135
                     /gene="miaA"
                     /locus_tag="PKZ53_10735"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNJ40955.1"
                     /translation="MNSIKPSQLQLIPVIVGPTCSGKSDLGIAIARHFGGEIINLDSV
                     QVYRGLYVATAKVPLEEQQGIPHHFIDMVEPTENYTAGRYAVDAARCLAEIEARHQMA
                     IFVGGTGFYLNALRGRLISEEEATDLTLRERLKSILTRRGPEHLHRMLTRLDPVSASR
                     LAPRDWSRVTRALEFFFQNRKRLSEQQPNLPDPPEFAARMRIIVLNPPRDVLYDKINR
                     RVDVMEQNGLLDEIKNLLAAGVPHDAKAFGAHGYRRIIEFLRGERTYESAIEQMKTDT
                     RHYAKRQITWWRREPDAAWFQGFGADSEIQQAVMDHLRVQGFGRDEG"
     gene            1405..1941
                     /locus_tag="PKZ53_10740"
     CDS             1405..1941
                     /locus_tag="PKZ53_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008278334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HNJ40956.1"
                     /translation="MPSGKTHDLITYILAIPTAVAFFLLTRNPGLTLLGTGAMLFSGL
                     MFGPDLDTRSVQYFRWGIFRGIWIPYQRLCGHRSRLSHGILFSTFFRIFYFLAILTAI
                     LAITLFIQNIWVLGMPPTADQGVKVVQESLFRMLKLIQSFDKRILILTVAGLWIGAMT
                     HSVSDFLGSTFKSASKFL"
     gene            2030..>2870
                     /locus_tag="PKZ53_10745"
     CDS             2030..>2870
                     /locus_tag="PKZ53_10745"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ40957.1"
                     /translation="MHLFALSGIRLGILCLALASAVSVCAADLMNPTALLPTGRPFFH
                     TCTDKTGLPQNSIQCMAFDQKGYLWIGTQDGAAVFNGRVWTVVNMPNRLESNFVRALL
                     TASDGSVWFGTDGGGVLHLKDGTWKTYGKSDGLPHNQVKCLIEATLPGGQRTILVATR
                     GGVSAFENGRWKPFDTPEKELAERGTLCLLESKDSTGTSTLWVGTQGKGLFRLENGSW
                     TRFDTSTGLIHNIVFCLLETRAANGAKTLWAGTYGGGLARFENGQWTGWNTSSGLPNN
                     IVLS"
BASE COUNT          656 a          698 c          776 g          740 t
ORIGIN      
        1 gaccaggaca aagaggaaaa aatatttatc caattgattt cagttccttt ttcgctaaag
       61 ttcggagtca atttggcgag agatgggagc ggcatcatac aagaactttc aattgtgcgc
      121 tactcgctcc tttgctcctc agtatttctg ttgctccact cccatgaatt caatcaaacc
      181 gtctcaactt caacttattc cagtgattgt cgggccgacc tgttccggga aaagtgatct
      241 tggaattgcg attgcccgac attttggcgg tgaaatcatc aatctggatt cagttcaggt
      301 ctaccggggg ctgtatgtcg cgacggccaa agttccactg gaagaacaac agggcatccc
      361 gcaccatttc attgatatgg ttgagccgac ggaaaactac acggcgggcc gctatgccgt
      421 ggatgccgcc aggtgtctgg ctgaaatcga agctcgtcac cagatggcga tttttgttgg
      481 cggaactggc ttttatctca atgcgttgcg tggaaggttg atttctgagg aagaagccac
      541 tgatttgact ctgcgtgagc gcctcaaatc tattctgact cgacgtgggc cagagcacct
      601 gcaccggatg ttaacgcggc tcgacccagt ttctgccagc cggctggcac cacgcgactg
      661 gtcgcgggtc acccgagcac tcgaattttt cttccaaaac aggaagcgtc tttccgaaca
      721 gcagccaaat ctcccagacc cacctgagtt tgctgcccga atgcgaatta ttgtgctcaa
      781 tccgccacgt gacgtgctct atgacaaaat caatcggcgg gtggatgtga tggagcaaaa
      841 tggcttactg gacgaaatca aaaatctgct ggcggctggt gtgccacacg atgcaaaggc
      901 ttttggtgcc cacggatacc ggcggatcat tgaatttcta cgcggagagc gcacctatga
      961 atccgccatt gaacagatga aaaccgatac tcgccactat gccaaacggc agataacctg
     1021 gtggcggcgc gagccagatg ctgcctggtt tcagggtttt ggtgcggact ctgaaattca
     1081 acaggcggtg atggatcatc tgagggttca gggtttcgga agggatgagg gatgagggat
     1141 gagggatgaa agaaatcacc ttttcaccct gttgttttat cctggttttc gccgcctagc
     1201 ggtggttgaa ctgagacagg tcgtttacgg cctgaaagat agaaaggaca taaattcgaa
     1261 aacccggaac ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccgaaata
     1321 atgacaaaat gataaaatga tgggatgatg ctatttcatt ttacatttcc cacccactga
     1381 cgcaggcggt actgactcct ccttatgcct tctggaaaaa cacacgattt aatcacctac
     1441 attctggcca ttcccacagc ggtggctttt tttctgttaa cccgaaatcc gggactgacg
     1501 ctgcttggaa ccggggcgat gttgtttagc ggtctgatgt ttgggccgga ccttgatact
     1561 cgaagcgtcc agtattttcg gtgggggatt tttcgcggga tctggattcc ttaccagcga
     1621 ctgtgtggtc accggtcacg gttgtcgcac ggaattttat tttcgacgtt ttttcgaatc
     1681 ttctattttc tggcgatatt gaccgctatt ttggccatca ccttgtttat ccagaatatc
     1741 tgggtgcttg gaatgccccc aactgctgat cagggagtga aagtggtgca ggaatcattg
     1801 ttccgcatgc taaaactcat tcaatcgttt gataaacgca ttctgatatt gacggtggcg
     1861 ggtttgtgga tcggagcgat gacccattct gtgagcgact ttcttggatc aacctttaaa
     1921 agcgcctcaa agtttctgtg atctgtgaga tacagttcat ccgagttgta tccaaagctt
     1981 gccattttct ttctgcacat cctgacttca tctttctttt tttcaggcca tgcacttgtt
     2041 cgcactgtct ggcatcaggc taggaatttt gtgtcttgct ctggcaagtg ccgtcagcgt
     2101 gtgtgcggct gacctgatga accccaccgc gttgcttcca accgggcgac cgttcttcca
     2161 tacctgcacc gataaaaccg ggcttccaca aaattcgatc cagtgcatgg cctttgacca
     2221 gaaaggctat ttatggattg ggacacagga tggggccgct gtttttaatg gccgggtgtg
     2281 gacggtcgtc aatatgccca accggctgga atcaaatttt gtacgggcgt tgctcaccgc
     2341 cagcgatggc agtgtctggt ttggcaccga tggtggtggc gtgttgcacc tcaaggatgg
     2401 gacctggaag acctatggga agtcagatgg cctgccgcat aatcaggtga aatgcctgat
     2461 cgaagcgacg ttacctggcg gccagcgaac gattctggtg gcaactcggg gcggggtatc
     2521 ggcttttgaa aatggccggt ggaaaccttt tgatacgcct gagaaggaac tggccgagcg
     2581 tggaaccctg tgtctgcttg aatcaaaaga ctcaactgga acctcaacgc tgtgggttgg
     2641 tacgcaaggc aagggcctgt ttcgattgga aaacgggtcg tggacccggt ttgataccag
     2701 cacaggtttg atccacaaca ttgtgttttg tctgcttgaa accagggctg ctaatggcgc
     2761 caaaaccctg tgggctggaa cctatggtgg cgggctcgcc cggtttgaaa acggtcagtg
     2821 gacgggatgg aacacttcgt ccgggctgcc caacaacatt gtgctgagcc
//
LOCUS       DAOQDO010000619         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28466_length_2870_cov_3.047602, whole genome shotgun sequence.
ACCESSION   DAOQDO010000619 DAOQDO010000000
VERSION     DAOQDO010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28466_length_2870_cov_3.047602"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKZ53_10750"
     CDS             <1..664
                     /locus_tag="PKZ53_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40958.1"
                     /translation="IRLDAPLDYYRNRLLVNAMVNFNMIDTIGSGVKRMFRKQRERNF
                     PLPDYDLSETGRVKVRIIGKVLDERYTRMLMLRSELNLLDVIALDKVQKEKSLTENEF
                     KSLKTKHLIEGRRPNLFISASVAAAIDMKDDYIKKRAFDKDHYKKMIVGYLEKFGEAS
                     RSELNKLLLNKLSDALNEEQKIQFVTNLLQDMRREGSVQSVGITRWAKWSLNKLSQKD
                     KN"
     gene            941..>2870
                     /locus_tag="PKZ53_10755"
     CDS             941..>2870
                     /locus_tag="PKZ53_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ40959.1"
                     /translation="MRLSPIRIFHLSLIFVFTLQSIGPLVVSARSISKTSPARNTVSP
                     PTRQSSTIRIRENNPVINENNQFTLTAIDANGQPVTGVTWESGSPDVASVDPQTGVVR
                     GVLRGYATVTARRGSETFSVFVTVVRVQGGNGAKVPGDTKTDTGGRLYISDPNGSIIL
                     RKDGFTSPATVFAGQRGANGRVDGPRLTARFNTPTAVAVDNNAQGGVYIADTANHSIR
                     KIGFDDRVLTTLGTGSPGMMSGDQTPFRAAVFRAPRGVASDTGGNLFIADTENHAIFY
                     VDFARQEVRLLAGAPGQSGKADGRGRAARFNRPTGVTMSTDGRFICVADTGNNRVRLI
                     TRDGQVATLGRNSTAKGSFFFPGQYALSTVKQVFDGDELVFDTPQSVGIDGLENIYVV
                     DKSGVQVVIRPAGQSPQVAQLAQIGTFQAAASVVVRGNESFVLDTSATTEAEAVKVVT
                     LGAPEITSLSQDNDRLEGGAEIVISGRNFAPESQVVLGDAVAEDATVESATQIRFRVP
                     AQTVPGLRTLSVQTRGGVAQRVFSIVAKSLSELKPGEITTVAGGVQFFGDGGSALNAG
                     FNFGAVESQFGGTVAIDGAGNFYVADHRNHRIRRVDQSGIITTIAGTGLRGFSGDNNL
                     AIRAALNNPNCVALDGAGN"
BASE COUNT          725 a          645 c          758 g          742 t
ORIGIN      
        1 gattcgtctt gacgccccat tggactatta ccggaatcgt cttttagtca acgccatggt
       61 gaatttcaat atgattgata ccattggaag tggggtgaaa cggatgtttc gaaaacagcg
      121 ggaacgtaac tttccactgc cggactatga tttgagtgaa acaggacgtg taaaagtacg
      181 aattattggg aaagtgcttg atgagagata tactcggatg ttgatgctga gatcagagtt
      241 gaatttactg gatgtgatag ctttagataa ggttcagaaa gaaaaatctt taacggaaaa
      301 tgaattcaaa tctttgaaaa caaaacactt gattgaggga agacgcccaa atctgtttat
      361 ttcggcatct gtcgccgcag ctattgatat gaaagatgat tatatcaaaa agagagcttt
      421 cgataaggat cactacaaaa agatgattgt cgggtatttg gagaaatttg gagaagcctc
      481 tcgaagtgaa ctcaacaaac ttcttctgaa taaactttca gacgccttga atgaagaaca
      541 aaagattcaa tttgtcacca atttacttca agacatgcga cgggaaggaa gcgttcaatc
      601 agttggtatt acccgttggg cgaaatggtc tttaaataaa ttgtctcaga aagacaaaaa
      661 ttagacatag atttatatat agactgaatt atgtaagtgg ataagtgatt tatttagaat
      721 aagacacgta tatataaatt ggatgtgatt cgtaaaaagc agcgcttgtt ataaggccct
      781 tctctcataa cacaggtaaa tttcatcagc actactctaa aattgcctcc tgaaaaaatt
      841 gctttacttt atctggattc taaatctgat attctaaatc tctttaaatc ttattgtttt
      901 cattgctagt tcctaacgcc cgcttaatag aggtccatct atgcgactat cacctatcag
      961 aattttccat ttaagtctca tttttgtttt cacccttcaa tcaatcgggc cacttgttgt
     1021 aagcgcccgg tcaatttcaa aaacttcgcc cgctcgaaac actgtttcac ctccaacccg
     1081 ccagagttcc acaattcgga ttcgcgaaaa taatccggtg atcaatgaaa ataatcagtt
     1141 taccctcact gccattgatg caaatgggca gccagtgact ggtgtgacct gggaatccgg
     1201 cagtccagat gtggcttcgg ttgatccgca aactggtgtg gtacgtggcg ttcttcgcgg
     1261 atatgccacg gtaacggcac gacgcgggag cgaaacgttt tccgtgtttg tgaccgtggt
     1321 cagagtgcag ggtgggaacg gagccaaagt ccctggcgat actaaaactg acacgggtgg
     1381 acgcctctac atcagcgacc caaacgggag cattattctc cgcaaggatg ggtttacttc
     1441 gccggccacg gtttttgctg gtcagcgggg cgcaaatgga cgggttgacg gcccacggct
     1501 gaccgcccgc tttaacaccc cgacggcagt ggccgtggat aacaatgctc agggcggtgt
     1561 ctacattgcg gatacggcca accactctat tcgcaaaatt gggtttgatg accgcgtgtt
     1621 gaccacgctc ggtactggct ctccagggat gatgtcaggt gatcaaacac catttcgtgc
     1681 ggctgttttt cgtgctccac gaggtgttgc cagtgacacc ggtggcaatt tgtttattgc
     1741 cgacactgaa aaccacgcca ttttctatgt agattttgcc cgccaggaag tccgcttgct
     1801 tgccggagca ccagggcaga gcggaaaggc ggatggtcgc ggacgtgcgg cgcgatttaa
     1861 ccgtccaacg ggtgtgacga tgagcacaga tggccggttc atttgcgtgg ccgataccgg
     1921 caacaaccgg gtgcgactca ttacccgtga tgggcaggtc gcgacgctgg ggagaaactc
     1981 cacggcgaaa ggctcattct tctttccagg tcagtatgct ctctccaccg taaagcaggt
     2041 gtttgacgga gatgaactcg tctttgatac gccgcaatcc gtcggtattg atggccttga
     2101 aaatatctat gtggttgata aaagtggagt tcaggtggtt attcggccag ccggccagtc
     2161 tccgcaggtg gctcaactgg cgcaaatcgg gactttccag gctgccgcga gcgttgtggt
     2221 tcgtggaaat gaaagttttg tactcgatac cagtgccacc accgaagccg aggccgtgaa
     2281 agtcgtgacg ctcggcgctc cggaaattac cagcttgagc caggacaatg accggcttga
     2341 aggcggcgcc gaaattgtga tttcaggtcg caactttgcg ccggaaagtc aggtggtgct
     2401 gggtgatgcc gtggccgagg acgcaacggt tgaaagtgcc acccagattc ggtttcgcgt
     2461 tccagcccag acggttcctg gtttgcgaac gctctcggtt caaacccgtg gtggtgtcgc
     2521 gcagcgagtg ttttcaattg ttgcgaaatc actttcagaa ttaaaacctg gcgaaatcac
     2581 cacggtggcg ggcggtgtcc agttttttgg ggatggcgga tccgccttga acgccgggtt
     2641 caattttggc gcggttgaaa gccagttcgg cggcacggtg gcgattgatg gcgccgggaa
     2701 tttctatgtt gcggaccatc gtaaccatcg catccgccgg gttgatcaat ctgggattat
     2761 caccacgatt gccggaacgg gattgcgtgg ttttagcggc gacaacaatc tggcaattcg
     2821 ggcagcgttg aataacccca attgtgtggc gctcgatggt gccggaaatt
//
LOCUS       DAOQDO010000620         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28489_length_2869_cov_2.998934, whole genome shotgun sequence.
ACCESSION   DAOQDO010000620 DAOQDO010000000
VERSION     DAOQDO010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28489_length_2869_cov_2.998934"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..931
                     /locus_tag="PKZ53_10760"
     CDS             83..931
                     /locus_tag="PKZ53_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40960.1"
                     /translation="MEHIAPVETERPVGNWVRQVLAIFRLETQKFLWGRRAFLMYALA
                     VLPVFILVLMVSFPFTSARLARSVVATEVFAWMFETLILRIMVFFGCAWIFMNLFRGD
                     VVDRCLHYYFLSPVRREVLVVGKYISGVVAATGLFVTSTAISLFLLYVPRGFHQGMDY
                     LLNGPGLKQAVLYLGIVTFACVGYGAVFLLVGLIFRNPIFPAIAFLGWETIGFLLPPI
                     LKKLSVLHYLKSLTPVPLSEGPFAIVAEPTPAWISIPGLLIVTAITLFFASILLRRME
                     IRYGGE"
     gene            1342..2802
                     /locus_tag="PKZ53_10765"
     CDS             1342..2802
                     /locus_tag="PKZ53_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNJ40961.1"
                     /translation="MNSIAEFVAAIDDSSVLLSHCLERLAVRNTQLADSYFEQVAIHT
                     DTIALAAERFGHQLKSHPMPIYDEIAVAMMIDLGELVRVAGWRLSEVEDDFFAKLRQC
                     LGEASAIFRGLAARAQGFERGIVPAQAIPTINLLRKVMEVPSRTEQLAALSAEPPPRE
                     SFPHDDPFDLLTIGEDDIGNFLEGIGESLDGSFDASFQGDDAQTEQDDETVRELFTEI
                     AMVFVRPMREFVAQVRLGRGGRDWIEPCLAACKSVRSAAQGVGYNSLSGHLEDFESVL
                     RKAENSYGNDIDTDALLRIEITDAYSELENFLPKVFSIPDDWNTPESVIISSILKQVQ
                     GVGRPAIRRLFSAGVSTLSAYVTAKPTDMAAAAGLPLAVAERIVERFRRYATEPRHRM
                     TVEDAQKARIEVIQSIVNELHSEQSRFLEAVSKEWHDPNEAPAKNESRRRRQEMMWEV
                     TVVLAEMGDTDLIDKLIRLPFDRRIELLEERLAELI"
BASE COUNT          619 a          717 c          747 g          786 t
ORIGIN      
        1 atttcaccat ttatcattca tcattcataa ttgatttcac tgcggaggat tcgtgagtag
       61 cacaccagta caggttaccc caatggaaca tattgcaccg gtcgaaacag agcgcccagt
      121 tggcaactgg gtgcggcagg ttctggccat tttccggctt gaaactcaga aatttttatg
      181 gggccgacgg gcgtttctga tgtatgccct ggcggtgtta ccagtattta ttctggtttt
      241 aatggtttcg tttccgttca catcggccag gctggccagg tcggtggtgg caactgaagt
      301 ttttgcctgg atgtttgaaa ccttgatcct gcgcatcatg gttttcttcg gctgtgcgtg
      361 gatttttatg aacctctttc gcggggatgt ggttgatcgg tgtttgcatt attattttct
      421 gtctccggtg cgacgcgaag ttctggtggt ggggaaatac atttccggcg tcgtggcagc
      481 caccgggctg tttgtgacgt caaccgccat ttcattattt ctgctctatg tcccacgcgg
      541 atttcatcag ggaatggact atttgctcaa tgggccgggg ttaaagcagg cggtgttgta
      601 tctcgggatt gtgacatttg cctgtgttgg ctatggcgcg gtgtttttac tggtcggttt
      661 gatctttcga aatccgattt tcccggccat tgccttcctt ggctgggaaa cgattggatt
      721 tttattacca ccgatcctca aaaaactgag cgtgttacat tatctcaaat ccttaacgcc
      781 cgttcctctt tccgaaggac cctttgcgat tgttgctgag ccgacaccgg cctggatttc
      841 cattcctgga cttttgattg tgactgccat tacgctgttt ttcgcgtcga tcctgttacg
      901 ccgcatggaa atccgctacg gaggggaatg aaaaatgaag aattgagaat ggaaaagtgg
      961 tgagcggtga gcggtgagtg gttagaaatc aatacgttcg aagaaaagtc agaagctgag
     1021 aaccaaccac tcaccactca ccgttcacca ctcaatggtt tcttcagtct caattcttca
     1081 gtctcaattc ttcagtctcc atcctccatt cctcattctt tattcctcat tctttattcc
     1141 caattctcaa ttcttcattc ttcattcttc atgagttgtg ctattttcat tgcaccactg
     1201 tgctgaacgt gttgaactat gctagttttg ggtagttcta aagcagcaat tgcaccactc
     1261 ttcgcagaaa ccatcttcga caagaacaaa gggagggcgt gataaccggt tttcgaactg
     1321 gaatatcttc gccggtttgc tatgaacagt atcgcagaat ttgtcgccgc cattgatgac
     1381 agttcagttc ttctttccca ctgcctggag cgtctggcgg ttcgcaatac tcagctcgcc
     1441 gattcatatt tcgaacaagt ggcgatccat acggatacga tagcacttgc tgctgagcgg
     1501 tttggccacc aactcaaaag ccatccgatg ccgatttatg acgaaattgc cgtggctatg
     1561 atgattgacc ttggcgaact ggtgcgcgtg gctggctggc gattaagcga agtggaagat
     1621 gatttttttg ccaagcttcg ccaatgtctg ggtgaagcat ccgccatttt ccgtggtttg
     1681 gccgcccggg cccagggatt tgaacgcgga atcgtgccgg cacaggcgat tccgacgatt
     1741 aacctgctcc ggaaggtgat ggaagttcca tcgcgtaccg aacaactggc ggctctctca
     1801 gccgaaccac cgccccgcga aagcttcccg catgacgacc cgtttgacct gttgacgatt
     1861 ggggaagatg acatcggtaa ttttctggaa ggaattggcg aatcactcga cgggtcattt
     1921 gatgcctcgt ttcaaggaga tgacgcccag accgagcagg atgacgaaac cgtgcgcgaa
     1981 ctgtttaccg aaattgccat ggtttttgtc cgaccaatgc gtgagtttgt cgcccaggtt
     2041 cgacttggac gcggcggacg cgactggatt gaaccctgcc tggcggcctg caaatcggtc
     2101 agaagcgctg cccagggtgt tgggtacaat agcctttcgg gtcacctgga agactttgaa
     2161 tcggtcttgc gcaaggctga aaactcgtat ggaaacgaca ttgacacgga cgccttgtta
     2221 cggattgaaa ttacggatgc ctattccgag cttgaaaact tcctgcccaa ggtcttttca
     2281 attcctgatg attggaacac gcctgagtca gtgattatta gctcaatcct caagcaggtt
     2341 cagggcgtcg gtcgcccggc gattcgtcgc ctgttttcgg ctggagtttc caccctgtca
     2401 gcttatgtca ctgccaaacc caccgatatg gccgccgctg ccgggctgcc gctggcggtt
     2461 gccgagcgga ttgtggaacg cttccggcgg tacgcaaccg agcctcgtca ccgcatgacg
     2521 gtcgaagatg cccaaaaagc ccggattgag gtaattcaat ccattgtgaa cgaactccat
     2581 tcagaacagt cccgcttcct cgaagcggtt tccaaagaat ggcatgatcc caacgaagca
     2641 ccagccaaga atgaaagccg tcgccgccgt caggagatga tgtgggaagt gactgtcgtc
     2701 ctggctgaaa tgggcgatac cgacctgatt gataaattga ttcgactgcc atttgatcgc
     2761 cgcattgaac tcctcgaaga acggctggcc gaactgatct gatgagctga gtgatggatc
     2821 agccttgaat ctcccctccc taccgctatg cggttgaggc gggggattc
//
LOCUS       DAOQDO010000621         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28522_length_2867_cov_3.563656, whole genome shotgun sequence.
ACCESSION   DAOQDO010000621 DAOQDO010000000
VERSION     DAOQDO010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28522_length_2867_cov_3.563656"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PKZ53_10770"
     CDS             complement(<1..490)
                     /locus_tag="PKZ53_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40962.1"
                     /translation="MRISLCFALLLCVLTSSASLLAQQPPSSQPPQTDPQVAKLVESI
                     RELQQYNTFVGVDERMFVVMAALNAAGFDVETPGMPPSSVRAQLRKDLGTIDPQLLIK
                     LQSFFKVHVQLEREPEAQATGYIALAFVLGAPPTFTPPADREFLANDVNELFEFAPLV
                     AEF"
     gene            complement(681..1805)
                     /locus_tag="PKZ53_10775"
     CDS             complement(681..1805)
                     /locus_tag="PKZ53_10775"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNJ40963.1"
                     /translation="MKSRILLLLGFCFLGLVVACTVDGQPATAQSPASTPAAKEPPAQ
                     SVPAATSASNIVFMGVGDIMLGSTYPDESGLPPNDGADLLKEATPILTSADLAFGNLE
                     GPMLEGGTTTKCGPKSTRCFAFRVPTRYGKHLKDAGIDVMSLANNHANDFGSQGMASS
                     KKVLDELGIAHAGEIGDIAYMTVKGRKIAIIGFATNAVSYNVNDIEIARRVVADTTKK
                     ADLVIVSFHGGAEGTANLHVPDQVEIFLGETRGHLRKFARTVIDAGADLVIGHGPHVC
                     RGMEVYKDRFIAYSLGNFATYGKFNLAEELGKTMILEVHIDPKDGAFRGGKIHPFKQI
                     KPGGPLADSSKAIIPLMKQLSESDFGDTSVKISDDGTISF"
     gene            complement(2078..2425)
                     /locus_tag="PKZ53_10780"
     CDS             complement(2078..2425)
                     /locus_tag="PKZ53_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ40964.1"
                     /translation="MSLKITQRTSGNVSILDLSGKITIGEGSVQLREAIRTLLDGNSK
                     YILLNLGDVSYVDSSGIGELVSSYTTVNKQQGSLKLLKLTKKIQDLLAITKLLTVFET
                     FDDEEKALASFPQ"
     gene            complement(2529..>2867)
                     /locus_tag="PKZ53_10785"
     CDS             complement(2529..>2867)
                     /locus_tag="PKZ53_10785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ40965.1"
                     /translation="VGLAVRESVINAIKHGNQLDSQKPVDVRFAIFEQHIDVSIRDRG
                     RGFDASHLPDPLDPSNLLNPNGRGIFYMRTFMDEVNFSTHPQGGTVVRMIKRKPETPQ
                     DADSDGLSAD"
BASE COUNT          707 a          707 c          763 g          690 t
ORIGIN      
        1 aaaactcagc gaccagcggt gcaaattcaa agagttcgtt gacatcgttg gccagaaact
       61 cgcggtcagc cggcggtgtg aacgtgggcg gagcaccaag cacaaaagcc agtgcaatat
      121 aaccggttgc ctgggcttcg ggttcacgtt ccagttgaac gtggacttta aaaaaactct
      181 ggagcttgat caggagttgc gggtcaattg tccccagatc tttgcggagc tgtgcccgaa
      241 cgctactcgg tggcatgcct ggggtttcaa cgtcaaatcc agcggcattc agtgcggcca
      301 tgaccacaaa catgcgttcg tctaccccga caaacgtgtt gtattgctga agttcacgaa
      361 tggattcaac cagtttggca acctgggggt cggtttgggg tggttgagag gatggtggtt
      421 gttgagccag cagggaagcc gaactcgtca gaacacacag gagcaaggca aaacaaagag
      481 aaatacgcat ggaaaccttg tacatgaggg ttcagggttc agggttcagg gtttggaaaa
      541 gaaaaggaca aaaggacaaa agattcgaaa acccggaacc tggaatcctg acaaggtgac
      601 aggatgacag ggtgacacgg tgatatttca tccctcatcc ttcatccctc atcccttctg
      661 aaaacccgga acccggaacc ctaaaatgaa atcgttccgt cgtcgctgat tttgacgctt
      721 gtatcgccaa aatcgctttc agagagttgc ttcatcagtg gaatgatggc tttggacgaa
      781 tcagcgagtg gtccgcccgg tttgatttgc ttgaatggat gaattttccc acctcgaaat
      841 gccccatctt tgggatcaat atggacttcc agaatcatgg ttttcccaag ttcttcagcc
      901 agattgaatt tgccataggt ggcaaagttc cccagtgaat aggcaatgaa gcgatctttg
      961 tacacttcca tccctcggca gacgtgaggt ccgtgcccaa tcaccaaatc agcaccagca
     1021 tcaatcaccg tgcgcgcaaa tttgcgaagg tggccacgag tttcacctag aaagatttcc
     1081 acctgatctg gaacatgcag gtttgctgtg ccttcggcgc cgccgtgaaa tgaaacaatt
     1141 accagatcgg cttttttggt ggtatcagcc acgactcttc gggcaatttc gatgtcattg
     1201 acattgtagg aaaccgcgtt ggtggcaaac ccgataatgg caattttgcg acctttgact
     1261 gtcatatagg caatgtcacc gatttcgcca gcgtgggcaa tcccgagttc atccagtacc
     1321 tttttggaac tggccattcc ctgcgagcca aaatcattgg cgtggttatt ggccaggctc
     1381 atgacgtcaa tcccggcatc tttgaggtgt tttccatacc gggttggcac tcggaaggca
     1441 aagcagcggg ttgatttggg gccacatttg gtggtggtgc caccttcgag catcgggcct
     1501 tcgagattcc caaacgcgag gtcagccgag gtcagaatcg gtgtggcttc tttgagtaga
     1561 tcagccccat cgttgggcgg cagcccagat tcgtccgggt aggttgaccc caacataatg
     1621 tcgcccacac ccataaacac gatatttgaa gccgacgttg ctgccggaac cgattgagct
     1681 ggtggctcct tcgcggctgg ggttgatgct ggcgactgag ccgtggcagg ttggccgtca
     1741 actgtgcagg caacaaccag tccaagaaaa caaaatccga gcaaaagcaa aattcgtgat
     1801 ttcatacaat tccagagtga cagtagtagg cggaagacat cgggcggaag acatcgggct
     1861 cagggctgag ggctgaagat tcgcaagctc agagcggaag acatcgggta taaaaagccc
     1921 tgagcccatt ttcttcagcc cgacgtcttc agcccgacgt cttcagcccc aggccagttg
     1981 tctttcattc acaaacacac aaacagcgag gcaggaaaaa tctcccaagc cccgctgtct
     2041 tagtcgccgt aaaaatgaaa tgtgatgcct ggcgtggtta ctgcggaaag ctggccagag
     2101 ctttttcttc gtcgtcaaag gtttcgaaga cggtcaaaag cttggtaata gccagcaggt
     2161 cctggatctt tttggtgagc ttcaacaact tcaaactgcc ttgttgtttg ttgacggtgg
     2221 tgtagctgct gacgagttcg ccgataccgg aactgtcaac atagctcaca tcgccaagat
     2281 tcagcaggat gtatttcgaa ttgccatcga gcagggtccg aatcgcttca cggagttgga
     2341 cactgccctc gccgattgtg attttcccgc tcaaatcgag gattgagacg ttgcctgagg
     2401 ttcgttgtgt gatcttcaga gacatgggcg ctgctcctta aaaaaggttg agtatgtgtt
     2461 aacttttttg aaagccctct gaccagtact cagactgggt gagaacagac ctgagtccgt
     2521 ttcacctttc aatcggctga aagaccgtcg gagtcggcgt cttgaggcgt ttcgggcttg
     2581 cgtttgatca ttctgaccac cgttccacct tgcggatggg tcgaaaaatt gacttcgtcc
     2641 ataaatgtcc gcatgtagaa aataccgcgt ccattggggt tcagaagatt tgagggatca
     2701 agcggatccg gtaaatgact tgcatcaaac ccccggccac ggtcccggat cgagacgtca
     2761 atgtgctgtt caaaaatcgc aaaccgcaca tcgactggct tttgtgaatc aagttgattg
     2821 ccatgtttga tggcgttgat gactgactca cgcaccgcaa gccctac
//
LOCUS       DAOQDO010000622         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28602_length_2863_cov_3.496439, whole genome shotgun sequence.
ACCESSION   DAOQDO010000622 DAOQDO010000000
VERSION     DAOQDO010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28602_length_2863_cov_3.496439"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKZ53_10790"
     CDS             <1..575
                     /locus_tag="PKZ53_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diketo-5-methylthio-1-phosphopentane
                     phosphatase"
                     /protein_id="HNJ40966.1"
                     /translation="TPNYWEQYFAGQMTHFDAMRGFFSHITCDEARLHEIARAMKPDP
                     DLAQAVARLRQTGWDIVVASAGCLWYIKYLLDQAGVSLIVHANPGFFEPGRGLVLELP
                     TDSPFLSTADGIDKTAVVQDALARYDIVAFAGNGPPDLSPAKCVPANLRFARGWLAET
                     LTKEGLGFRPYQRWAEVANFLAHHSDKGEL"
     gene            569..1621
                     /locus_tag="PKZ53_10795"
     CDS             569..1621
                     /locus_tag="PKZ53_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HNJ40967.1"
                     /translation="MVIRSVIRVPEVVRIKPGALDRLGLYLYRASHFQITLFLSAGAP
                     EGPVNQALKSLDQGMIRVQCRIEVVDASVEEAVEQLSNLPLHCQAIVGLGGGKALDTA
                     KYVASLAGLPYYAVPTSLSNDGFCSPQSSLTLAGKRKSLVSAMPFAVVIDTAVCLQAP
                     VELWCSGIGDLAAKFTAVTDWKLAFHAQNTPVNDLAALLSDATVFQFIGNPERTVKGV
                     KLLATALMLNGMAMEMAGSSRPASGSEHLISHALDVISTRPRLHGLQVGVAAYLVSHL
                     QRGPSKIIAELFEKTGFWDVIRQDPFQWNEWIGAINLAPILKEGFYTVLSSRDCLPEL
                     EQLIETDPWLQGVFIK"
     gene            1811..2362
                     /locus_tag="PKZ53_10800"
     CDS             1811..2362
                     /locus_tag="PKZ53_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40968.1"
                     /translation="MTFRSAVVSFFCVILISSAACRASQSATEPSKETSPVKPVVEMK
                     APDASASPAIVADDQKAATDPEKAWKPFLASFRAAVKKQNVASLKTMMVKSFEYVGGT
                     IAPEEFLEDFEDDEDWGELQKTVALEDKPYAHPTSKRPSRIIKNLNPCGGKKPCKYEL
                     WAVFEFGEDNQWRWTAYLAPEKK"
     gene            complement(2532..>2863)
                     /locus_tag="PKZ53_10805"
     CDS             complement(2532..>2863)
                     /locus_tag="PKZ53_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40969.1"
                     /translation="ANLTQNIDRTILESEQEALAQVIQSGNQPETVPINRNRAQENLI
                     PIVRRLQEKHGPDTVDLLENPTAPGIDKARVCRILIDLYREILALPQENSAEISRMML
                     SEAFLGE"
BASE COUNT          672 a          684 c          733 g          774 t
ORIGIN      
        1 atacgccaaa ctattgggag caatatttcg ctggtcagat gactcacttt gacgccatgc
       61 gcggtttttt ctcacatatc acctgcgacg aagcccgcct ccacgagatt gcccgtgcga
      121 tgaaacccga tccggatctg gctcaggccg ttgccaggct tcgacagacc gggtgggaca
      181 ttgttgttgc ctcagccgga tgtctctggt acatcaagta tttattggat caagccggcg
      241 tatcactaat cgtgcatgcc aatcccggct tttttgaacc aggacgcgga ctggtacttg
      301 agcttcccac cgattcgccc tttctttcca ccgccgatgg gattgataaa acggctgttg
      361 ttcaagatgc gcttgctcgc tatgacattg tggcatttgc tggcaatgga ccacccgatt
      421 tgtcacccgc caaatgtgtt cctgccaatc tgcggtttgc ccgtggctgg ctggccgaaa
      481 cattgacgaa agaagggctt ggctttcgac cgtatcaacg ctgggctgag gttgcaaact
      541 tccttgccca ccattcagat aaaggtgaat tgtgattcgc agtgtgattc gtgttcctga
      601 agttgtgcgc atcaagcccg gcgcactgga tcgattgggg ctttatttgt atcgggccag
      661 tcattttcaa atcacattgt ttctgagtgc tggtgctccg gaagggcctg tcaatcaggc
      721 gttaaaaagc ctggaccagg gtatgatcag agttcaatgc cggattgaag tggttgatgc
      781 cagcgttgaa gaggccgttg agcaactgtc aaatctccct cttcattgtc aggctattgt
      841 tgggttaggc ggaggtaaag cccttgatac agcaaaatat gttgccagcc tggctggttt
      901 gccgtattac gccgttccga cttcgctttc aaacgatggg ttttgttcgc cacaatcaag
      961 cctgactctg gctggcaaac gcaaatctct ggtttcagcc atgccgtttg cagtggttat
     1021 tgacacggct gtttgcctgc aggctccggt tgaactctgg tgttcgggaa tcggtgatct
     1081 ggcggccaaa tttactgcgg tgacggattg gaaactggcc ttccacgccc agaatacacc
     1141 agtcaatgat ttagcggcat tgctgtcaga tgcgactgtg tttcaattca ttgggaatcc
     1201 agagcgaacg gtcaaaggag tgaaattgct tgccactgcc ttgatgctca atggcatggc
     1261 gatggaaatg gccggttctt cgcgcccagc cagcggtagc gagcacctga tttctcatgc
     1321 ccttgatgtg atcagcactc gaccccggtt gcatgggctt caggttggtg tggctgctta
     1381 tctggtcagc catttacaac gcggaccttc aaaaataatt gcagaactct ttgaaaaaac
     1441 agggttttgg gatgtcatcc gtcaggaccc ctttcaatgg aatgaatgga taggggccat
     1501 taatcttgcg ccaattctca aagaagggtt ttataccgtg ctttcatcgc gcgattgcct
     1561 tccagaactg gagcaactga ttgagactga cccgtggcta caaggtgttt ttatcaaata
     1621 ataccggtca gtcgtcagtc gctcgccaaa cttatttgat tgaataactt caatgttttc
     1681 tcatccaggg tattcattgt caaaatagta catagctgaa aaatatgtat ttcctcatac
     1741 cctggtccga taccctcaac ccgaaatggc aaacagcgat aaaattatga aacgaaagga
     1801 tttttgtact gtgacttttc gatctgcagt cgtgagtttc ttctgtgtga ttttgatttc
     1861 aagtgcggcc tgtcgtgcca gccagagtgc gacggaaccg tccaaagaga cctcaccggt
     1921 caaaccggtt gttgagatga aagctcctga cgcttcagcc tcacccgcta tcgtggctga
     1981 tgatcaaaaa gcggcaaccg acccggaaaa agcctggaaa ccttttctgg cttctttccg
     2041 cgccgccgtc aaaaagcaga atgtcgcctc cctcaaaacc atgatggtta agtcatttga
     2101 atatgtcggc ggcaccatcg cgccggagga atttttggaa gattttgagg atgatgaaga
     2161 ttggggtgag cttcaaaaaa cagtggcgct tgaagacaag ccgtatgccc atcccacgag
     2221 caaacgtccc agccggatta tcaaaaatct caatccgtgc ggcgggaaaa aaccttgcaa
     2281 atatgaattg tgggcagttt ttgagtttgg cgaagacaac caatggcggt ggacagccta
     2341 tctggcaccg gagaagaaat agcgcatacc agtcagtcgt cagtcgtcga gtgttgggat
     2401 tttggaattg gtgtcaagtg tatttggttc tatttgagtc agttatgaat aattcttttc
     2461 aagaattcca cacactacaa tatccgggtt ttatcgactg acgactgact attgactaca
     2521 aactgatatc actactcacc caggaaagct tcagacagca tcatgcggga aatttcagcc
     2581 gagttttcct ggggaagcgc caggatttca cggtacaggt caatcaatat ccggcagaca
     2641 cgcgctttat caattccagg tgcggttgga ttttcaagca gatcaaccgt gtccggccca
     2701 tgtttttcct ggagccggcg gacaatcggg atgaggtttt cctgtgcccg gttccggtta
     2761 atcggaaccg tttctggctg gtttccactt tgaatgacct gagccagtgc ttcttgttcc
     2821 gattcaagaa tggtgcggtc aatattttgg gtgagattgg cgg
//
LOCUS       DAOQDO010000623         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28627_length_2862_cov_3.050588, whole genome shotgun sequence.
ACCESSION   DAOQDO010000623 DAOQDO010000000
VERSION     DAOQDO010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28627_length_2862_cov_3.050588"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2862
                     /locus_tag="PKZ53_10810"
     CDS             <1..>2862
                     /locus_tag="PKZ53_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNJ40970.1"
                     /translation="PQSILAVTFTNKATEEMRHRLRRMVPADAQRLSVGTFHSFCIRV
                     LRQHYEALNMPKHFAVTDEKGQLSVLQKVVPTLRDTNDTKRLLGQLSSWRMSETTQRS
                     SLTEFQEETLKKYLTELRRQALIDFDDILLLTFKLFHKHADVLKHQQAQYQSILVDEF
                     QDTDLLQYRILKQLTGLQTPVFAVADDDQSIFAWRGANPENLRQFVADYLSSDSEPHV
                     IKLEANYRCSGSIVAAANRMIAPSLRLFDKVPTAVNETGGPVEVREFDTDQEEAEAIA
                     QEIEDLVQDPGQNLTYRDLAILYRRHQIGEVLELTLLSHGIPCQVVRGASLFDLPRIQ
                     RVLALMRVQLNPKDEISLQALVKEYLDDLLFHKVEALARSCGSLRLALWKRLNASDGD
                     HAERRQIGRVVGMLSTAKALYEMQPGLSLSEWVQALNLYLEAEVVQRVQDTASQQDLQ
                     RISDPLGIPALETAARWLERLGMRQGVLVIAATDEANELAAAFLLKPLLASAPFSIST
                     WRAIEANPVPKGKPMLLLALDPEAEKEVLHQHQFIAILAVTTVDSPLPKSNPPKRNLP
                     TKLIPAGEAGPQPSMFVTLWKLGQAYQGLRSNVFLPDYTAVDIETTDLDLERCDIVEL
                     AAVRVRNGQVVAEFSELLKPSVPISPQAEAVHHISASMVAAAPTFEQVAAEFRAFIGS
                     DTLVAHNGLGFDFPIIMRRLRTAAQAAQQPAQGLENPFFDTLPMARQLYPEAKKATLE
                     SLAARFEVDPGSSHRALDDTRTLALVFERMKTDYAEAQRARNGFDTLGCLAAAMLLEM
                     DELSAEKSPLFRLGRRQWTSENTEDLVKTLATAAEDRDDGSGEQLQAAWHMVIQECAD
                     SQLSRDAAQETESYLRFLELLKRYDGRPLAEVIQEFIDFTRLFQQQDSWKERNAVTLM
                     TIHAAKGLEFPWVWIAGVEQGVIPTYQAIKAEGKLRAAKV"
BASE COUNT          649 a          769 c          817 g          627 t
ORIGIN      
        1 cctcagagca ttctcgccgt taccttcacc aacaaagcga ctgaggaaat gcgccaccgg
       61 ctacgtcgga tggtgcctgc tgacgctcag cggctctcgg tgggaacgtt ccactcgttt
      121 tgtattcggg tgctgcggca gcattatgaa gccctcaata tgccaaaaca ctttgctgtc
      181 accgatgaaa aaggacagct cagtgtgctc caaaaagtgg tgccgacgtt gcgcgatacc
      241 aatgacacaa aacggttgct tggacaactc agctcatggc ggatgtcaga aacgactcag
      301 cggtcttcgt tgactgagtt tcaggaagaa acactcaaaa aatatctcac cgagcttcgc
      361 cgtcaggcac tgattgattt cgatgacatt ttgctgctga cctttaagct ctttcacaag
      421 cacgccgacg tcttaaagca tcagcaggca cagtaccaat ccattttggt tgatgaattt
      481 caggacaccg acctgcttca gtaccgcatt ctgaaacaac tcacgggttt gcagacccca
      541 gtgtttgctg tggctgacga tgaccagtcc atttttgcgt ggcgtggggc caatccggaa
      601 aaccttcggc aatttgtggc agattattta tcgagtgaca gtgaaccgca cgtgatcaag
      661 ctcgaagcca actatcgctg cagtggctca attgttgccg ccgccaaccg gatgattgcc
      721 ccaagcttgc gtctgtttga caaagtgcca acggcggtca atgaaacagg tgggccagtg
      781 gaagttcgcg aatttgacac cgaccaggag gaagccgaag ccattgccca ggaaatcgaa
      841 gacctggttc aggatccagg ccagaacctg acctatcgcg atctggcgat tttgtaccgt
      901 cgccaccaga ttggcgaagt gctggaactg acgcttctga gtcacgggat tccctgtcag
      961 gtggtgcgcg gggccagttt gtttgacctg ccgcgcatcc agcgtgtgct ggccttaatg
     1021 cgagtgcaac tcaatccgaa agatgaaatc agtttacagg cactggtcaa ggaatatctt
     1081 gacgatctgt tgtttcacaa agtcgaagcg ctggcacggt catgtggctc gcttcgtctg
     1141 gcgctgtgga agcggttgaa tgccagcgac ggcgatcatg ccgagcgacg ccagattggc
     1201 cgggtggttg ggatgctttc gacggccaaa gcgctctatg aaatgcagcc cggactttcg
     1261 ctcagcgaat gggtccaggc gctcaatctg taccttgaag ccgaagttgt acagcgggtt
     1321 caggatacgg ccagccagca ggatttgcag cggatttctg acccactcgg cattccggcg
     1381 cttgaaacgg cagcgcggtg gctcgaacgg cttggaatgc gtcagggcgt gcttgtgatt
     1441 gccgccaccg atgaagccaa cgaactggct gccgcgtttc tgctcaagcc gctgctggcg
     1501 agtgccccgt ttagtatcag cacctggcgg gcgattgagg ccaatccggt accaaaaggc
     1561 aaaccgatgt tgctgctggc actcgatccg gaagccgaga aagaagtgct gcaccagcat
     1621 cagtttattg ccattctggc cgtgacgacg gttgattcac cacttccaaa atccaacccg
     1681 cccaaacgca atctgccgac caaactgatt ccagccggtg aagccggacc acaaccttcg
     1741 atgtttgtca cactgtggaa actgggccag gcgtatcagg gcttgcggag caatgtcttt
     1801 ttacctgatt acaccgccgt ggatattgaa acgactgacc ttgaccttga gcggtgtgac
     1861 attgtcgaac tggcggctgt gcgggtgcgc aatggccagg tcgtggctga attttccgaa
     1921 ttgctcaagc cgtccgttcc tatcagccca caggctgagg cggtgcatca catcagtgcc
     1981 tcgatggtgg cagccgcccc cacctttgaa caggtcgcgg ctgaatttcg ggccttcatc
     2041 ggttctgaca cgctggtggc acacaacgga cttgggtttg atttcccgat cattatgcgg
     2101 cggctccgga cggcggcaca ggcggcacaa cagccagccc agggacttga aaatccgttt
     2161 ttcgacacgc ttccaatggc gcgccagctt tatccggaag ccaaaaaagc cacgctggaa
     2221 tcgctggctg cccgatttga agttgacccc ggctccagtc atcgtgcgct ggacgatacg
     2281 cgcacgctgg cgctggtttt tgaacgaatg aaaaccgact atgccgaggc ccaacgggct
     2341 cgcaacggct ttgacacgct cggatgtctg gcggcggcga tgctgctgga aatggacgaa
     2401 ctttcagccg agaaatcgcc actctttcga cttggccgcc gccaatggac ctcggaaaac
     2461 accgaagatc tggtcaaaac cctggcgact gcggctgaag atcgggatga tgggagcggc
     2521 gagcaattgc aggcggcgtg gcacatggtg attcaggaat gtgccgacag tcagttgtca
     2581 cgtgatgccg cgcaggaaac agaaagttat ttgcggtttt tggaacttct gaaacgctat
     2641 gacggacgac cgctggccga ggtgatccag gaattcattg attttacgcg tctctttcag
     2701 caacaggata gctggaaaga acgcaatgcc gtgacattga tgaccattca tgcggcgaag
     2761 ggcctggaat ttccctgggt atggattgcg ggtgtcgagc aaggggtgat tccgacctat
     2821 caggcaatca aggccgaagg caagctccgg gcggccaaag tt
//
LOCUS       DAOQDO010000624         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28644_length_2861_cov_3.501069, whole genome shotgun sequence.
ACCESSION   DAOQDO010000624 DAOQDO010000000
VERSION     DAOQDO010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28644_length_2861_cov_3.501069"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKZ53_10815"
     CDS             <1..400
                     /locus_tag="PKZ53_10815"
                     /inference="COORDINATES: protein motif:HMM:NF025808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HNJ40971.1"
                     /translation="SEISKIVKGSKNMANTDSSQQNDRIEELNEQLIEAEEIIEELDI
                     EATSKWKCKPQARIYRIRVDKHYFKVDRSQVTGKEILTIAGKVPPKNFLLYQVDCAGK
                     SDEISLNETVDLSKPGIEKFRTLPRDQTEG"
     gene            405..803
                     /locus_tag="PKZ53_10820"
     CDS             405..803
                     /locus_tag="PKZ53_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HNJ40972.1"
                     /translation="MRRDFMLPETDRVFLEREFKTWETILQDHKRWLILRDQSVPDGY
                     QQKTVDLALMIETNYPIGQLDMIYVYPTLIRMDGKTISNTQAKQPLEGKQYQRWSRHR
                     TKENPWRPGEDCVETHLNLVHDWFRRELEK"
     gene            888..2291
                     /locus_tag="PKZ53_10825"
     CDS             888..2291
                     /locus_tag="PKZ53_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNJ40973.1"
                     /translation="MTNLSLSMTESQRQILHQHLFDKDGNEGVAIALCGHHQEGDRQR
                     VLVQQIYLIPHNACHVRKPDLISWPTKLIVPFLEEAAKRSLSVVKFHGHANAWPFFSQ
                     TDNTSDLSLHESLSGWTVPGVFHGSAILLQDGRIFGRWYGDDDNFHWMDRITVVGEMI
                     EIFFGKDFSSQPSDCTQSLGLDRHAKAFGWKTTQLLQQLNIGVVGCSGIGSPVIEQLA
                     RLGVGKLVLVDPDCVEERNLNRILNATKADADAHRPKVQVLTEAIQKIGFGTTVIGLE
                     ASLFERRAVLALAGCDLIFGCMDRAEGRALLNRLATFYILPYFDLGCKIEADGNGEIQ
                     SVTTAVHYLQPGKSSLLSRGAITRETIEAEGLQRTDPDRYEELKREKYIIGVQEERPG
                     VISVNLAAAAFGVNEMLSRLHDFRTDGNSGFAQCRLSLSHMALYPGPETDDCPALKRD
                     IGRGHIEPLLDYPQLSL"
BASE COUNT          779 a          653 c          697 g          732 t
ORIGIN      
        1 ttctgagata tcaaagatag ttaaaggatc aaagaatatg gcaaacactg actctagtca
       61 acaaaatgac cgaattgaag aactcaatga acaactcatt gaagcagaag aaatcatcga
      121 ggaacttgac attgaggcta cttctaaatg gaaatgtaaa cctcaagccc gtatttatcg
      181 aatccgggtt gataagcatt atttcaaagt ggatcggtcc caggtcaccg gcaaggaaat
      241 tctcactatc gccggaaaag ttccacccaa gaatttcctt ctttaccagg ttgattgtgc
      301 cgggaaatca gacgaaatca gtctgaatga gacagttgat ctatcgaagc cgggaattga
      361 aaaattccga actttgccaa gagaccaaac ggagggatag ttgtatgcgg cgtgacttta
      421 tgcttcccga aacggatcgg gtatttctcg aacgtgagtt caagacctgg gaaaccattc
      481 tccaggacca caagcgttgg ttgattttac gggatcaatc ggttccagat ggctaccaac
      541 aaaagacagt tgacttggct ttaatgatag aaacgaacta tccgattggc caactcgaca
      601 tgatttacgt ctatcctacc ctgatccgaa tggatggaaa aaccatttca aacacccagg
      661 ccaaacaacc cttagaagga aaacaatacc aacggtggtc acggcatcga actaaagaga
      721 atccttggcg accaggggaa gattgtgtcg aaacccattt gaacttagtt cacgattggt
      781 ttcgaaggga gcttgaaaag tgaagcaccc cgaattcgtt gaaagggata ccgtcaaagt
      841 gtgccgttct gtatcggaat cccttttggc ttacgagata tggaaatatg acgaatcttt
      901 cgctctccat gaccgagtcc cagcgccaaa ttctccacca gcacctgttt gacaaagacg
      961 gaaacgaagg cgttgcaatt gccctttgtg gccatcatca ggaaggtgac cgacaacggg
     1021 ttctagttca gcagatttat ctcatccccc acaacgcctg ccatgtgcgt aaacccgatt
     1081 tgatttcctg gccaaccaaa ttgattgttc cgtttttgga agaagcggcc aaacgcagtc
     1141 tcagtgtggt taagtttcat gggcatgcca atgcctggcc attcttttcc caaaccgata
     1201 acacgtcaga tttaagcctc cacgaatcac tgtcgggatg gactgtccca ggcgtgtttc
     1261 acggtagtgc cattctgctc caggacggtc ggatttttgg ccgatggtat ggcgatgatg
     1321 acaattttca ctggatggac cgaattactg ttgtgggtga aatgatcgaa atctttttcg
     1381 gaaaagactt ttcctcccaa ccatccgatt gcactcagtc acttggcctt gaccgccatg
     1441 caaaggcttt tgggtggaag accacccagt tgctccagca gttgaatatt ggagtggttg
     1501 gttgttccgg aatcggaagt cccgtaattg aacagttggc tcgcctgggc gttggtaaat
     1561 tggttctggt tgatccggat tgtgtcgaag aaaggaatct caaccggatt ctgaacgcta
     1621 cgaaagccga tgccgatgct catcgtccca aagtccaggt cctgactgaa gcgattcaaa
     1681 aaattgggtt cggaaccacc gtcatcgggt tggaagccag tttgtttgaa agacgggcgg
     1741 ttttggcact ggcagggtgc gaccttattt tcggctgtat ggatcgggct gaagggagag
     1801 ctttattaaa tcggctcgca accttttata tcctgcccta ctttgatctc gggtgcaaaa
     1861 ttgaagctga cggaaatggc gaaatacaat ccgtcaccac cgccgttcac tatttacaac
     1921 ctggaaaatc tagtcttctc agccggggtg ccattacccg tgaaacaatc gaggctgagg
     1981 gcttgcaacg gaccgatcca gaccgttatg aagaattgaa acgggaaaag tacattatcg
     2041 gagttcagga agaacgacct ggcgtcataa gtgtaaattt ggcggcagcg gcctttggag
     2101 tcaacgaaat gctttctcgg ttgcacgatt ttcggacaga cgggaattca ggttttgccc
     2161 aatgtcggct aagcctttcg catatggcac tttacccagg cccggaaaca gatgattgcc
     2221 ccgctttaaa acgcgatatc ggacgtggcc acattgaacc gctccttgac tatccccaac
     2281 tgagcttatg aaggttattt tgaatccaat caaacgattt tgggagtggg tgtgggaaac
     2341 tatccgaagt tggtttcgaa aacggcaacc cggttttcgt ttccttgaag ttgaggaggt
     2401 tccggaaaaa atgaacgact caattgtgta tttggtgggg gaaaaagggc acatctggtt
     2461 tgccgccatg aaatgtcctg gaggttgtaa tactgttatt gaacttaatt tggttcgcac
     2521 atctagaccc tgctggacaa tcgaaaaaca tccagaccga acccttacca ttcatccttc
     2581 aatcttgcgg atcagaggct gtcactgcca tttttggctt cgcaatggcc aaattacttg
     2641 gtgttcctcg ccagaggttc caggaacagc ctcacaccgc cgctgataac tgccttcggc
     2701 cattttgctc aaggtaggaa attaggggtt aacgtgtatt gtcccaaagc aggtaccaga
     2761 cacatctaca ggaaacttct acagggaaag gtcaattttt atcaactatt tgcagggaat
     2821 caggttttcg agggtagcgg tcagtgacct tcccgataat t
//
LOCUS       DAOQDO010000625         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28646_length_2861_cov_3.427655, whole genome shotgun sequence.
ACCESSION   DAOQDO010000625 DAOQDO010000000
VERSION     DAOQDO010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28646_length_2861_cov_3.427655"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2833)
                     /locus_tag="PKZ53_10830"
     CDS             complement(<1..2833)
                     /locus_tag="PKZ53_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNJ40974.1"
                     /translation="MVFRGNPETFAPSNARTQKLKAEGTFTESPLLETGVVIDLPNRP
                     DFLGAGDFNGDGTFDLVTAARGESQLHLLPGNGTGDFGNPTTVEVAGPVTALTVGDVN
                     RQDGLADVLVSIADTTAARLLVFEGPDGALRHEPEVFTLPAPATAIAVGELGGSPFSD
                     IAVAAGNTLLVAEGRDRKLSVSDKRIEPLRINQRTFDFEIKSLALGDFEADDLTDVAV
                     LSTDGQVEVLSREMPAALRQSASLDYDIVNNGQDEVKTTVSVPSGVVAATKALRAPVR
                     LSQWNATHFADVGTQANQLLRIKSSSLSHDDLAVIGEASDIRLLGNLDQDQSSANKRA
                     SAPASLQVTLPSRTGAPVAILPMLLNGDGLQDLVVVTRNTARPGVIFTQPLVVYTVNS
                     TTDTGTGVGTSGDLRYCITQANLSAGADSINFAIPGSGVQTITLASSLPFITQPLTID
                     GYTQTGASANTQAVGSDAVLQIEINADALVVNTPAVPFNNGSTGSRIRGLVINRANDN
                     QASAITFNQSSTGIVDGNFLGTSADGTTSLPNSRGVSIFNASTITIGGTTPQTRNVIS
                     GGASSIGINVTGGTGHLIQGNYVGLNAAGTAAIDAGFTTGMFLLGAPTTNGFVGGTAA
                     GAGNVVGARGIGVYVADFSSNMTVQQNLVGLNAAGTASLAATAIGMQVEDTASALIGG
                     TTAAARNVVGGTSSIGITVVDTSTSAQVQGNYVGTNSAGTTAIANSVGVSITRSTTGH
                     TIGGTAAGAQNVISGSSFEGIQITQTNGPHTVQGNLIGRNAANTAPLGNGTGIGLGTS
                     VFGANNILVGGTGAGEGNIIAHSTGVGVRIEGGTSNRILGNTISGSASLGIDLVPAGG
                     NGVTPNDTGDPDAGPNNVQNFPVITPISATSVSASLDSLVANTAYPVRLEFFANAACK
                     SVGTSEGEVFLGTTNLAGPGSVTFNFTAVP"
BASE COUNT          639 a          821 c          772 g          629 t
ORIGIN      
        1 ctggaactgc ggtaaagttg aaggtaacac ttccgggccc ggccagattc gttgtaccaa
       61 gaaatacttc gccttcactt gtccccaccg acttacaggc cgcattggca aagaattcaa
      121 gccgtaccgg gtaggcggtg ttggcgacca gactatcaag cgaagcactc accgatgtag
      181 ctgaaatcgg ggtaatcacc ggaaagtttt gcacgttgtt tggcccggca tccgggtcgc
      241 cggtatcgtt tggtgtcacg ccgttgcccc cggctggaac caagtcaatg cccagactcg
      301 ccgagccaga aatggtattc ccgagaatgc gattactggt gccaccttca atccggacac
      361 caactccggt gctatgggca atgatgtttc cttcaccagc cccagttccg ccaaccagaa
      421 tattattcgc gccgaacact gatgtcccaa ggccgatccc ggtgccattt ccaagcggcg
      481 cggtattggc ggcattgcgc ccgatgagat tgccttgaac ggtatggggg ccgttggtct
      541 gagtgatttg gattccctca aaactactgc cgctgatgac gttttgggca ccggcagccg
      601 ttccaccgat ggtgtgacct gtggtacttc gggtaatgga aaccccaacg gaattcgcaa
      661 tcgcggttgt cccggcagaa ttggtcccga cgtaatttcc ctgaacctgg gcactggtac
      721 ttgtgtcaac cacagtgata ccaattgagc ttgtgccgcc gacaacgttg cgggcagcag
      781 cggtggtccc tccgatgagg gccgaagcgg tatcttccac ttgcatgcca atggccgtcg
      841 ccgccaatga tgctgttcca gcggcgttga ggccaaccag gttttgctgt accgtcatat
      901 ttgacgagaa atcagccaca tacaccccaa tgcctctcgc tccgacgaca ttccctgccc
      961 cagcggcggt cccgccaaca aagccgttgg tggtgggtgc acccagcaga aacataccag
     1021 tcgtaaaacc agcatcaatg gctgcggtcc cagccgcatt gagccccaca taattgccct
     1081 ggatcaaatg accagtacca ccggtgacat tgataccaat cgaactggca ccaccactga
     1141 tgacatttcg ggtttgaggt gtcgtgccac caatggtaat tgtgctggca ttgaaaatgg
     1201 aaacaccccg gctatttggc aaggaggtcg ttccatcggc ggatgtgcct aagaagttcc
     1261 catccactat cccagttgaa ctctgattga aggtaatggc tgaagcttga ttgtcattgg
     1321 cgcgattgat caccagccca cggatccgac tgccagtgct cccattgtta aatggcaccg
     1381 ccggcgtgtt gacaaccagg gcatcggcat tgatctcaat ttggagcacc gcatcgcttc
     1441 caaccgcctg ggtgttggct gaagccccgg tttgggtata accgtcaatc gtcagcggct
     1501 gggtgataaa gggcaagctg gaggccagag taatcgtctg gacaccgctt cccggaatcg
     1561 caaagttgat gctatcagcg ccagcgctga gattggcctg agtaatgcag tatcgtaaat
     1621 caccactggt ccccaccccg gtgcccgtgt cggtcgtgct gttgacggta tagaccacca
     1681 gtggctgggt gaaaatcacg cccggacggg cggtattgcg cgtcacaacc accagatcct
     1741 gcaaaccatc tccgttgagc aacatcggca gaatcgcgac cggggcgccg gttcgagacg
     1801 gcaaggtaac ctggagcgaa gctggtgccg aggcacgttt gtttgcggac gattgatcct
     1861 ggtccaggtt tccaagcagt cgaatgtctg aggcttcgcc aatgactgcc agatcatcgt
     1921 gcgacaggct ggaacttttg attcgaagaa gctggttggc ttgcgttccg acgtcggcaa
     1981 agtgtgtggc gttccactgg gataatcgaa ccggggcgcg caaggctttg gtcgccgcaa
     2041 caactcccga aggaactgac actgtggttt tcacctcgtc ttgaccattg ttcacaatgt
     2101 catagtcaag tgaagctgat tgacgcaggg ctgccggcat ctcgcgtgag agcacctcaa
     2161 cctggccgtc agttgacagc actgccacat cggtcaggtc atcggcttcg aaatcgccca
     2221 aagccagcga tttaatctca aaatcgaacg tccgctggtt gattcgcaat ggttcaatcc
     2281 gtttgtcact cacggaaagt tttcggtcgc gaccttcggc aacgagcaac gtgtttccag
     2341 ccgcaacggc aatgtcagaa aaaggacttc cgcccagttc gccaacggca atggcggtgg
     2401 ccggagccgg caatgtgaac acttccggtt catggcgtaa ggcgccgtct ggtccttcaa
     2461 acaccagcaa acgcgcggct gtggtatcgg caatcgacac cagcacatcg gccaaaccat
     2521 cctgacgatt gacatcaccc acggtcaacg ccgtcaccgg gccggcaact tcaaccgttg
     2581 tgggattccc gaaatcgccc gttccatttc cagggagcag gtgcaactgg ctttcacctc
     2641 gcgccgccgt taccagatca aaagtaccgt ctccgttaaa atctccagca ccaagaaaat
     2701 ccggacgatt tggaaggtca atgacgacac cagtttccag cagcggtgac tcggtaaacg
     2761 ttccttcagc tttgagtttc tgggttctgg cattgcttgg ggcaaaggtt tctggatttc
     2821 ctcgaaacac cataaccgtg ccaccagttg ccgtttcata c
//
LOCUS       DAOQDO010000626         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28675_length_2859_cov_3.233951, whole genome shotgun sequence.
ACCESSION   DAOQDO010000626 DAOQDO010000000
VERSION     DAOQDO010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28675_length_2859_cov_3.233951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..>2859
                     /locus_tag="PKZ53_10835"
     CDS             43..>2859
                     /locus_tag="PKZ53_10835"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ40975.1"
                     /translation="MHAFFRFSAFFLTLSLVVVNVATSQPVFAQRKAGGLNLRGLILD
                     ESGAGLAGAEITLTTSAGKNLQSVSGEKGGFAFDGLAPGEYLVKVSAPGFAPYQSEVI
                     QLGEGQPTPLVIQMAIETVAEQVTVDANQPLSLSADNNASAVVFKGADLETLPEDPDE
                     LASALQELAGPGGGPGGAQLFVDGFSGIRLPPKSSIREIRINSNPFSAEYDRLGFGRI
                     EILTKPGIEQLRGEAFFNFNDESLNARNAFAPFRGPLQVRRYGGNLSGSIIPKKASFF
                     VDFERRDTDENASVNATILDANLQPVSFSDVIVTPRRFTTFSPRVDWQVNDKTTLVVR
                     YNLNNNSSQNQGIGNFSLETRGFDTTNRTHTLNFTATTIFSPQVINEVRLQMTRQSTR
                     SLGDNSLPSINVSGSFNGGGAQVGENERSEQRFEFQDYVTIALNAHSIKTGVRLRGIR
                     SSDSNTANFGGAYTFAGDVNRDPVTGQPIGGTITSLEQYRRVLLGLPGYRPSQFTLNA
                     GDPFVSVNQYDAGAFFQDEWRVRDNLTLSMGLRYEAQTNLSAKYNFAPRVAFAYAPRQ
                     GTNSSGKTLGTVIRGGFGIFYDRFDESLTLGTRRLDGNHVTQFFTTQPDFFPNIPDPS
                     FLNAQQPIRRQIAEGLEAPYIMQAGIGIEQQLPFNLTTSVNYVWSRGIHQLRSRNVNA
                     PLPGTFTGIPGSGTRPFGNIGDIYLYEATGRSETHQIRVGVNRRLANNFSIFSNYTWT
                     NAKSDTEDAGAFPVSSYDLSTEWGRTGFVAPHQLFMGGSTTLPWGIRLNSFINLRSGR
                     PFNITLGRDLNGDTVFTERPSFAQPGTPGSITTDFGTFSLNPKPGETLIPRNYGDGPG
                     FASVNVNLSKTFNLGAKSKNETAGGQTGETRIPGGFGGMGGGGRGGGFGGGGPRGGGP
                     GGGFGGDGGGDTRYSLTFSIRVSNL"
BASE COUNT          648 a          789 c          751 g          671 t
ORIGIN      
        1 gaacccggaa cccggaaccc ggaaccccaa tttggaaata ccatgcacgc attctttcga
       61 ttttctgctt tttttctcac tctttcactg gtcgtggtaa atgttgcgac cagccagccg
      121 gtgtttgccc agcgcaaagc tggtggtctc aatctgcgag gcttgatcct tgacgaatcc
      181 ggtgccggat tggccggtgc cgaaattaca ctcaccacca gtgccggaaa aaatttgcaa
      241 tcagtttcag gcgaaaaagg cgggtttgcc tttgatggat tagcaccggg tgaatatctg
      301 gtgaaggtct cagcgccggg gtttgctccc tatcagagcg aagtcattca acttggggag
      361 ggacaaccaa cacccctggt gatccaaatg gctattgaaa ccgtcgccga acaagtcacc
      421 gttgatgcca accagcccct gagcctgtcg gctgacaaca atgccagcgc cgtggttttt
      481 aaaggtgctg atttagaaac gcttccggaa gatcctgacg aactcgccag cgcactccag
      541 gaactggctg gtcccggcgg cggtcccggc ggcgcccagc ttttcgtgga tggattttct
      601 ggtattcgat tgcctccgaa aagctccatc cgtgaaatcc ggattaactc caacccgttt
      661 tcagccgaat atgaccgtct gggctttggc cgaattgaaa tcctgaccaa acctggaatc
      721 gaacagcttc gcggcgaagc ttttttcaac ttcaatgacg aaagtttgaa tgcccgcaat
      781 gcgtttgctc catttcgtgg accgttgcag gtccgacggt atggcggcaa cttgtcaggt
      841 tcgatcattc ccaagaaagc ttcgtttttt gtggattttg aacgccgtga cactgatgaa
      901 aacgcttcgg tcaatgcaac gattctggac gccaacctcc agccagtgag cttttcagat
      961 gtcattgtga caccgcgccg gtttaccact ttttcacctc gggttgactg gcaggtaaat
     1021 gacaaaacaa cgctggtggt tcgatacaac ttgaataaca actccagcca gaatcaggga
     1081 atcggcaatt tttcacttga aacccgtggc tttgacacca cgaaccgaac ccacaccctg
     1141 aattttaccg ccacgaccat ttttagcccg caggttatca atgaagtccg gctgcagatg
     1201 acccgacaat ccacccggtc gctgggtgat aattccctgc catccatcaa tgtttcaggg
     1261 tcttttaatg gcggcggtgc ccaggttggc gaaaatgaac ggtccgaaca gcggtttgaa
     1321 tttcaggact atgtgacgat tgcgctcaat gcccattcga tcaaaaccgg agttcggctg
     1381 cggggcattc gttcgtcaga tagcaacacg gccaattttg gtggcgccta cacatttgcg
     1441 ggcgatgtca accgggaccc ggtaaccggc caacccattg gcggcacaat taccagcctc
     1501 gaacaatacc gccgggtgct gcttgggctt ccggggtatc gtccgtcgca gttcacgctc
     1561 aatgccggag acccgtttgt ttccgtcaat caatatgatg ccggggcgtt tttccaggac
     1621 gaatggcggg tccgtgacaa cctgacgctt tcgatggggt tgcgctatga agcccagacc
     1681 aacctgagcg ccaaatacaa ttttgctccg cgagttgcgt ttgcctatgc accgcgccag
     1741 gggacaaaca gcagcggaaa aacgctggga acggtcattc gtggtggatt cggaattttc
     1801 tacgaccggt ttgacgaaag cctgacgctc ggaacgcgtc gtctggacgg caaccacgtc
     1861 actcagtttt tcacgacgca acctgacttc ttcccaaata ttcccgaccc gtcgttcctc
     1921 aatgcccagc agccaatccg acgccagatt gccgaagggt tagaagcccc gtacattatg
     1981 caggcgggaa ttggcattga gcagcaactc ccatttaatt tgacgacgag tgtcaactac
     2041 gtctggtcgc gtggcattca tcaacttcgg tcacgcaacg tcaatgcgcc gcttccgggg
     2101 acatttacag ggattcccgg atctggaacc cgcccgtttg gtaacattgg cgatatttac
     2161 ctgtatgaag cgacgggccg ctctgaaacc catcagattc gagtcggtgt caatcgccga
     2221 ttggccaata atttctcgat tttttcaaat tacacctgga cgaatgccaa aagcgacacc
     2281 gaagacgccg gggcatttcc ggtcagtagc tatgacctga gcactgaatg gggacgcacc
     2341 ggatttgtcg caccacacca gttgtttatg ggcggttcga ccacgttgcc ctggggaatc
     2401 cggctcaatt cgtttatcaa cctgcgttcg ggccgaccat ttaacattac ccttgggcga
     2461 gatttaaacg gagacacggt ctttaccgaa cggccttcgt ttgcccaacc tggaactccg
     2521 ggcagcatca cgacggactt cggaacattt agcctcaatc ccaaacctgg cgaaaccttg
     2581 atcccacgca attatggaga cggtcccggc tttgcctcag tcaatgtcaa cctgagcaag
     2641 acctttaacc tcggtgccaa atccaaaaat gaaacggctg gtggccagac cggtgaaacc
     2701 agaattcctg gcggatttgg cggaatgggc ggcggcggac gcggcggcgg atttggtggt
     2761 ggcggtcccc gtggaggtgg tcccggcggt ggctttggtg gtgatggcgg cggcgatacc
     2821 cgctatagcc tgacgttctc gattcgagtt tcaaacttg
//
LOCUS       DAOQDO010000627         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28703_length_2858_cov_2.434534, whole genome shotgun sequence.
ACCESSION   DAOQDO010000627 DAOQDO010000000
VERSION     DAOQDO010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28703_length_2858_cov_2.434534"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKZ53_10840"
     CDS             complement(<1..504)
                     /locus_tag="PKZ53_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40976.1"
                     /translation="MRLAFIALLTWWLALPTPVFPKKVLASQTITGQITQLEAYLSPA
                     ETRRANGILTLVGQILTKCGEDWYSNHPINEFDELCFKLKAFGSPASRFLWVRNQIGV
                     LNNSYQTFEPNQPKQAQPVGFLEESDYNSFSEIIVTSSADSGPGTLREAVEQANRVGG
                     SRRIIFRL"
     gene            complement(523..1707)
                     /locus_tag="PKZ53_10845"
     CDS             complement(523..1707)
                     /locus_tag="PKZ53_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40977.1"
                     /translation="MKKLNAHRPQFLNRRHRGYILPCTLLICFLILSWLLVLGLETTK
                     NVQIIRHERYRVQAQSVSFAVQPDLKVAAEAFFVRTACALEEEWQQKFAKQTPPTREE
                     VRQFIDDRFPFYQPIEMFYATLVQGKPEVVASGYRQVYGQGMIKLDFDAIEVRTVGGQ
                     PNRVVIPFTMVIPYRISFASRDLAPSPQAFLDTYGSIWHPPTGVLFDAKTVDKTNDIS
                     VLTKPNYDNVIQYRAELVLVLEKPGVEPPVWNPPAGIRPGKPGSCPVTVQRNPDGTCE
                     IVNCNGDNVSGGGTFSDCEEAQAVADAYNQNPAQGQEAFQTAVSEKSFKSFCINNFGC
                     AQPTLVDSSQYTNLEDYGETVDGDVPIDNLGVPPGSLIREVKVRFPKEGFHRGIPRRM
                     RF"
     gene            complement(1791..2231)
                     /locus_tag="PKZ53_10850"
     CDS             complement(1791..2231)
                     /locus_tag="PKZ53_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNJ40978.1"
                     /translation="MKRQRRISHTAGYTLLELVIAIGILLVTVSLVGSFMYTDLRGHQ
                     TIEDSLKLNRLATQVAQRLAVQKIKADAAGQITIDSDGNIVAFGESKEETRRPVNPGP
                     GRKNPGEMVYGYTVSVRAISGGITHVDVFLNAGQGRDVFASATY"
     gene            complement(2329..>2858)
                     /locus_tag="PKZ53_10855"
     CDS             complement(2329..>2858)
                     /locus_tag="PKZ53_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40979.1"
                     /translation="AGGAGIPFPHHQRQEMGLSLLELVIVLGGLAALSIGILGIVQQY
                     GRGQNDHEVKAAEAIITNLEKARLNARLGIDQSADRTVTPSAIRIPASVELVTKVGDG
                     ELPAGVTLLNPRQAIVFDPQSGLLNGGSGMIVVQDRVTKRARGILIPKVYGPLQRYVK
                     FPRDQRFTLMNSVVY"
BASE COUNT          674 a          790 c          642 g          752 t
ORIGIN      
        1 aagtctaaaa ataattcgtc tactcccccc aactcgattg gcttgctcga cagcttctcg
       61 taatgtccca ggcccagaat cggcactact tgtaactata atttctgaaa aagaattata
      121 atctgactct tccagaaatc cgactggttg tgcttgctta ggttgattgg gctcaaaggt
      181 ttgataggaa ttattgagaa ctccaatttg atttcttacc cataaaaatc gacttgctgg
      241 tgaaccaaag gcttttaatt taaaacacaa ctcatcaaat tcgttgattg ggtgatttga
      301 ataccaatct tcaccacatt tggttaggat ttgtcccact aatgtcaaaa tcccgtttgc
      361 tcttcgcgtt tccgcagggg aaagataagc ttcaagttgg gttatttggc cagtgatagt
      421 ttgtgacgcc aaaacctttt tggggaatac cggagttggc aaagcaagcc accaagttaa
      481 gagagcaata aaagccaatc gcatagattt tcctaattac aattaaaacc gcattcttct
      541 tggaattccg cgatgaaacc cctctttggg aaatcgaact ttcacttctc tgataaggga
      601 gccagggggt acacccaagt tatcaattgg aacatcgcca tctacagttt ctccataatc
      661 ctccaggtta gtgtattgtg atgaatcaac taaggttggc tgagcacatc caaaattatt
      721 gatacaaaag gatttaaaag atttttcaga gaccgccgtt tgaaaggctt cctgcccttg
      781 tgctggattc tgattatagg catcagccac agcctgggct tcttcgcagt cgctgaatgt
      841 cccgccacct gagacattgt ccccattgca gttcacaatt tcacacgtcc catccgggtt
      901 acgctggact gtcaccggac acgatcctgg tttgccaggt cgtatcccag ccggtggatt
      961 ccaaaccggt ggctcgactc ccggtttttc caacaccagc accagttctg cccggtactg
     1021 gatcacattg tcgtagtttg gtttggtcaa gacactgatg tcatttgtct tatcaaccgt
     1081 tttggcgtca aacagcactc ccgtcggtgg atgccagatt gatccgtatg tgtccagaaa
     1141 tgcctgtggc gatggcgcta aatcccttga tgcaaacgag atccggtacg gaatcaccat
     1201 cgtgaacgga atcaccaccc gattcggctg gcctcctact gttcggacct caatcgcgtc
     1261 aaagtccagt ttgatcatcc cttgaccgta cacctgtcgg tagccgctcg ccaccacttc
     1321 cggcttcccc tgcaccagcg tcgcgtaaaa catctctatt ggctgataga atggaaaccg
     1381 gtcatcaatg aactgccgca cttcctcccg tgttggtggt gtctgtttcg caaatttctg
     1441 ctgccattct tcttccagtg cgcaggctgt ccgtacgaaa aacgcttctg ccgccacttt
     1501 caaatctggc tgcaccgcaa atgataccga ttgcgcctgc acccgatatc gttcgtggcg
     1561 gataatttgc acgttcttcg tggtttccaa tcccaacacc agtaaccagc tcaggatcaa
     1621 aaagcagatt aacaacgtgc agggcaaaat gtatccgcga tgacgccgat tgaggaactg
     1681 agggcggtgt gcattgagct ttttcataaa ttatcgtctc catccggagc caatggtctg
     1741 taaatacttg taaatgagtg ttttgactca gagttgattc ttcgactgat ttaataggtt
     1801 gctgaggcaa acacgtcacg tccctgcccg gcgttcagga aaacatccac atgcgtgatg
     1861 cctcccgaaa tggcccgcac tgaaaccgtg tagccataca ccatttcgcc cggattcttt
     1921 cggccaggac ctggattgac tggcctcctg gtctcttctt ttgactcccc aaacgccaca
     1981 atgttgccgt cagaatcaat cgtaatctgt cccgccgcat cggctttgat cttttgcact
     2041 gccagccgct gggcaacctg tgtcgccagc cgattgagtt tgagcgaatc ctcaatcgtt
     2101 tgatgaccac gcagatcggt gtacataaag ctccccacca gcgacaccgt caccagcaaa
     2161 ataccaattg caatcaccag ttccagcagc gtgtacccag ccgtgtgaga aattcttctt
     2221 tgacgtttca tcagttcctc ctcaaaactc gcctgctctg gcagggagtc ctgatgttga
     2281 cccgtgtttt gggtttgtcc tcaagccctg agccccaagc cccaggattt aatacacaac
     2341 cgaattcatc agcgtaaagc gttgatccct gggaaatttc acgtagcgtt gcagtggacc
     2401 atagactttt ggaatcagga ttccacgggc gcgtttggtg acccggtcct ggaccacaat
     2461 catcccgctc cctccattca gcaggccgct ttgtggatca aacacaatgg cctggcgtgg
     2521 gttgagcaag gtgacacctg cgggcaattc gccgtccccg actttggtca ccagttccac
     2581 cgaagccgga atccggatcg cggagggcgt taccgttcga tccgcactct gatcaatccc
     2641 caaccgggca ttgagccggg ctttttcaag gttggtgatg atcgcctctg ccgctttgac
     2701 ttcatggtca ttctggccac gcccatattg ctggacaatc cccaggattc caattgacaa
     2761 cgccgccagt ccgcccagca cgatcaccag ctccagcagg ctcaacccca tttcctgccg
     2821 ttgatggtga ggaaacggta tgcctgcgcc cccggctt
//
LOCUS       DAOQDO010000628         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28757_length_2855_cov_3.095000, whole genome shotgun sequence.
ACCESSION   DAOQDO010000628 DAOQDO010000000
VERSION     DAOQDO010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28757_length_2855_cov_3.095000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKZ53_10860"
     CDS             complement(<1..1043)
                     /locus_tag="PKZ53_10860"
                     /inference="COORDINATES: protein motif:HMM:NF020846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HNJ40980.1"
                     /translation="MKWGSILCLILGVSWCWSPSVAHLATGSADRGRAVVPTDAAVVL
                     RQATRLSGMSPEVQLQPVTLVLSPRDEVELFHRLTELQTPGSPRYHQWLTPEEFGARF
                     GRTEVEYQQLIDWLEAQDLEVVATWPNRLQITVIGSVEVVERAFRVTMNFYQLGDLEG
                     YAADREASLPVSLVPLVQGIVGLQNFPRVEAVQQPAAPGNILGPADLATVYRLKPLFD
                     KGLDGTGTTIAIVARADFSLDDVRQFRSQFGLPPKEVSKVFPFGPITNPDPLDMVEAL
                     VDCEWAGAAAPDANITAVIAPELYLSVQSIVNDLPQAQCINISFAGCELLNAKPLADL
                     FQALFAQATAQGQT"
     gene            1089..>2855
                     /locus_tag="PKZ53_10865"
     CDS             1089..>2855
                     /locus_tag="PKZ53_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ40981.1"
                     /translation="MKIACQMRPAERHLSDQLWNLSGSGRMRLLPVIYFVSYSQSFII
                     ITGVATAAPCFHSVGELPVQTIQQPEKYHGLQPQELVEMYRTMYLSRRIDDKEIQLKG
                     QNKIYFQISGAGHEAILVAAGKVMKPGYDWFYAYYRDRALCLQLGMTALEMLQAAVGA
                     EADPNSGGRQMPSHWGHKDLNIVSKSSCTGTQFLQAVGCAEASFRHSELKYTDLPLKT
                     SFHADEVVYCSSGDGTTSEGEFFEALNTACNLKLPVVFVIEDNGYAISVPIEVQTAGG
                     SISKLVRGFPNLLILEVDGTDPLASYAVMREAVGYARARRGPALVHAQVIRPYSHSLS
                     DDEKLYRLPGERESESKRDPIKTFADFLVTENILTAEGLAALKAQVEKELTEATDAAL
                     ASPQPAPETATQYVFSPDVDPTSADFDSPATFDADKPLTVVDLLNTCMHDEMARDPRI
                     VVFGEDVADASREEVLTEVKGKGGVFKVTHNLQRKFGGTRVYNSPLAEANIIGRAIGL
                     ATRGFKPVVEIQFMDYIWPAFMQLRNELALMRWRSNNAFAAPVVVRVPTGGYLKGGAI
                     YHSQCAEALFTHTPGFRVVFPSN"
BASE COUNT          682 a          798 c          739 g          636 t
ORIGIN      
        1 gtttgccctt gcgcagtcgc ctgggcaaag agtgcctgaa acagatcagc cagcggtttg
       61 gcatttaaca attcgcaacc ggcgaagctg atattgatgc actgggcctg gggcaggtca
      121 ttgacgatgc tttggactga taaatagagt tccggagcaa tcaccgccgt aatgttggca
      181 tcgggtgccg cggccccggc ccactcacaa tccaccagag cttcgaccat atccagcggg
      241 tccgggttgg tgatcggccc aaacgggaaa accttgctga cttccttggg gggcaaccca
      301 aactggcttc ggaattgacg cacatcatca agactgaaat cagcccgcgc cacaatggca
      361 atggtggtcc cggttccgtc aagccctttg tcaaaaagcg gctttagccg atagacggtg
      421 gccagatcag ccggccccaa aatatttcct ggagctgccg gctgctgaac ggcctcaacg
      481 cgcgggaaat tttgtaatcc gacaatcccc tgcaccagcg gtaccaaact gactgggagc
      541 gatgcctctc gatctgctgc atagccttcg agatcaccga gttgatagaa attcatggtc
      601 acccgaaaag cacgctccac aacttcaacc gacccaatga ctgtgatttg aagccgattg
      661 ggccacgtcg ccaccacctc caaatcctgg gcttcaagcc agtcaatcag ttgttgatat
      721 tcaacttcgg ttcggccaaa acgcgcgcca aattcttcgg gtgtcagcca ttgatgatag
      781 cgtggcgagc ctggtgtttg gagttcagtc aatcggtgaa acaactcgac ttcgtcgcgg
      841 ggtgataaaa ccagcgtcac gggctggagc tggacttcag gtgacatacc tgaaagccgg
      901 gtcgcctgtc gaaggactac ggcggcgtca gtcggcacca cagctctgcc ccggtcagcc
      961 gacccggtcg ccagatgagc aactgagggt gaccagcacc agctaactcc cagtatcaag
     1021 cacaggatac ttccccattt catagtttcc cccagataac tgagcttgaa aaacgagttg
     1081 ttgcgtcaat gaaaattgcc tgccagatgc gcccggcaga gcgacatcta tcagaccagt
     1141 tgtggaacct gtctggttcc ggtaggatgc gacttcttcc cgtcatttat tttgtttcat
     1201 acagccaatc attcatcatc atcactggtg ttgcgacggc agcaccatgt tttcattccg
     1261 taggagaatt acccgtgcaa acgattcagc aacctgaaaa ataccacggc ttgcagccac
     1321 aggaactcgt cgaaatgtac cgcacgatgt atctctcacg tcgcattgac gacaaagaga
     1381 tccagctcaa aggtcaaaac aagatctatt ttcagattag cggtgctgga cacgaagcca
     1441 ttctggttgc agccggaaaa gtcatgaagc caggctatga ctggttttat gcctattacc
     1501 gtgaccgcgc gctgtgctta caactgggaa tgacagcatt ggaaatgctg caggccgcag
     1561 ttggcgctga agccgatcca aattccggcg gtcgtcaaat gccgtcacac tgggggcata
     1621 aagatctcaa cattgtctcg aaatcaagct gcaccggcac ccagtttttg caggcggtgg
     1681 gatgtgccga agccagcttt cggcactcgg agttaaaata caccgatctg ccgttgaaga
     1741 ccagttttca cgcagatgaa gtggtgtatt gctcatctgg cgatggcacc accagtgaag
     1801 gtgaattctt cgaggcgctc aatacggcct gcaacctcaa acttccggtt gtgtttgtca
     1861 tcgaagacaa cggctatgcc atttcagtcc cgattgaagt tcaaaccgcc ggcggcagca
     1921 tttccaagct ggtgcgaggc ttcccaaatt tgctcatttt ggaagttgac ggaacggacc
     1981 cgctggccag ctatgccgtg atgcgtgaag ccgtgggcta tgctcgtgcc cggcgtggcc
     2041 cggcgctggt gcatgcacag gtcatccggc cttattcgca ttccctgtca gatgatgaaa
     2101 agctctatcg tctgcctggt gaacgtgaat cagaatccaa acgcgatcca atcaaaacct
     2161 ttgctgattt tctggttacc gaaaatattt tgaccgccga gggtctggca gccctcaaag
     2221 ctcaagtcga aaaagaattg actgaagcca ccgatgcggc gctggcctct ccgcaaccag
     2281 cccctgaaac ggcaacccag tatgtttttt cacctgacgt agacccaacc tcagccgatt
     2341 ttgacagccc ggccaccttt gacgccgaca aacctctgac cgtagttgac ctgctcaata
     2401 cctgtatgca cgacgaaatg gcacgcgatc cccggattgt ggtctttggc gaagatgtgg
     2461 ccgatgccag tcgtgaagaa gtcctcactg aagtcaaagg caaaggcggc gtgtttaaag
     2521 tcacccacaa cctgcagcga aaattcggcg gaactcgtgt ctataactcg ccactggctg
     2581 aagccaacat tattggtcgg gctatcgggc tggcgactcg cggattcaaa cccgtggttg
     2641 aaatccagtt tatggattac atctggccag cttttatgca actgcgaaac gaactggccc
     2701 tgatgcgctg gcggtcaaac aatgcgtttg cggcacccgt cgtggttcga gtcccaaccg
     2761 gcggctatct caaaggcggc gcgatttatc acagccagtg cgccgaagcc ctctttaccc
     2821 acacaccggg ctttcgggtt gtgttcccaa gcaat
//
LOCUS       DAOQDO010000629         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28784_length_2853_cov_3.396712, whole genome shotgun sequence.
ACCESSION   DAOQDO010000629 DAOQDO010000000
VERSION     DAOQDO010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28784_length_2853_cov_3.396712"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKZ53_10870"
     CDS             complement(<1..274)
                     /locus_tag="PKZ53_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ40982.1"
                     /translation="MSVSLQKGQKISLAKEAGATLTRVIMGLGWDAKKKGGFFGFGAK
                     TQEIDLDASCLLFDQNKQVVDVVWFRQLRSRDGSIQHTGDNRTGQGE"
     gene            complement(304..882)
                     /locus_tag="PKZ53_10875"
     CDS             complement(304..882)
                     /locus_tag="PKZ53_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ40983.1"
                     /translation="MPISLSKGGNVSLSKAEPGLKKVLVGLGWESRATDGAGFDLDAS
                     VFLLADSGKVRADNDFIFYNNLSSADGSVVHSGDNRTGAGAGDDEVVRVNLGGVPADV
                     KRIVFAVTIHDAEARRQNFGMVSNAFIRVVNDDNTNEIARYDLSEDSSTETAMIFGEL
                     YRHGEEWKFKAVGQGYNGGLGPLAQSFGVNIG"
     gene            1190..>2853
                     /locus_tag="PKZ53_10880"
     CDS             1190..>2853
                     /locus_tag="PKZ53_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40984.1"
                     /translation="MYLRFTLRLFAILWVLGSGVICLAAPQSVKTRFAAQGTTQVFQL
                     KIPLADIAPNSAWDITARASDGTAFAVAVDRPFPTQVATEWTSPEYQKQYEAYQERVN
                     QVSIKLNQVNEQISTLEPQQKALADLLKDTTIKAETRTVLQAKLNRVEAEHKALLKKR
                     SDLEAEMARVQESAPKFDAEPKSESKPEPQWLEVYGRLHGTEPASVILATVPRSGEPA
                     RVVATFKLSPPQKFPTDAELVKQWATTWTNQLGFELVNSGDPWMHLQLIRQTTQKYQI
                     PMPDIGQYVQMQRRGDTRFQDDVDLFSITTGGLAIQESLQYKRLLNISENRGGQTVPI
                     TKLEGPAVKSHPFEKMLEGRQPVQFELAKLVPHDQYYVHFSTIKSEIELSDLLDQWGT
                     TLLQSVEVTSHDRQVKERLLTQLCLELSSLTRLFGSFVVDDLALTGNDPFVTDGNDVS
                     VIFNVKNGPIFDQSMAAHRAEAKKQRPDAVESTARYGTFEIGTLTTPDRTISSYWTAV
                     DSYRIYSNSRSALERILDAAQGKRPSLKAAAEFRYMRTIFPGTASE"
BASE COUNT          732 a          822 c          662 g          637 t
ORIGIN      
        1 cttcgccctg accggtgcgg ttatcgccag tgtgctggat gctgccgtca cggctgcgca
       61 actggcggaa ccaaacgaca tccaccacct gcttattctg gtcaaacaac aaacacgagg
      121 catcaaggtc aatttcctgg gttttggcgc caaagccaaa aaagccgcct ttctttttgg
      181 catcccaccc aaggcccatg ataaccctgg tcagcgtggc accagcttct ttggccaatg
      241 aaattttctg tcctttttgt aaactgacgg acatggttca ccttccttta tcgagtttga
      301 gtcttatcca atattgacac caaaggactg tgccagcggc ccaaggccac cgttgtaacc
      361 ctgaccgacc gccttgaatt tccattcttc gccgtgacga tagagttcac caaagatcat
      421 cgcggtttca gtgctggaat cttccgacag gtcatagcgc gcaatttcgt tggtgttgtc
      481 atcattgacg acccgaataa acgcatttga gaccattccg aagttttgcc gacgggcttc
      541 agcatcgtga atcgtcaccg caaaaacaat gcgtttgaca tccgctggaa cgccacctag
      601 attcacccgc acaacttcgt cgtcaccagc cccagctcca gtccgattgt cgccgctatg
      661 caccactgaa ccatctgcgg aacttaaatt gttgtagaaa ataaaatcgt tatctgcccg
      721 gacttttccg gaatcagcca gcaaaaagac actcgcatcg aggtcgaaac ctgcaccatc
      781 agtggctctg gattcccatc caagtcctac caatactttt ttcaaacccg gttccgcttt
      841 gctcagcgat acatttccgc cttttgaaag tgaaattggc ataaacttct ccctaatgtg
      901 aaagttttta aagtaagtta tccggcctcc aaccgatgtg ggaaaaaccg gtaattttcg
      961 gagcacgaaa gtaccccaac tgttacaggt tggcaagtcg cttcttgact gaacccggtt
     1021 tcccaagcga caaatccagc aagaaccacc accactcaac tatttttcaa tcaacacgcc
     1081 gcaattccgg atagataagt cattcaaaac agtacatttg ccagccaatt cactcgtttt
     1141 gaccgactca aaactggaat atttcaccac cagactttgt gaggatccta tgtatctgcg
     1201 gtttacgctt cgattgtttg ccattttatg ggtgcttggc agcggtgtga tttgcctggc
     1261 agcaccacaa tccgtcaaaa cccggtttgc ggcgcaagga acgacccagg tttttcagct
     1321 caaaattcca ctcgctgaca ttgccccaaa cagtgcctgg gacattaccg cccgtgcctc
     1381 agacggaacc gcctttgcgg ttgccgttga ccgcccgttc ccgacccagg tcgccacaga
     1441 atggacatca cctgaatatc aaaaacagta tgaagcctat caggaacgag tgaaccaggt
     1501 aagcatcaaa ctcaatcagg tcaacgaaca gatttctacg cttgaacctc agcaaaaagc
     1561 cctggctgat ctcctcaaag acaccacgat caaggctgaa acccgcactg tgttacaggc
     1621 caaactgaat cgggtggaag ccgagcataa agcgcttctc aaaaaacgga gtgacctcga
     1681 agctgaaatg gccagggtgc aggaatcagc ccccaaattt gatgcggagc ccaaatccga
     1741 atcaaaacca gagccgcagt ggcttgaggt gtatggtcgg cttcacggaa cagagccagc
     1801 ctcggtcatt ctcgccacgg tgcccagatc aggcgagcca gcgcgggtgg tggcgacctt
     1861 caagctgtca ccgccccaaa aattcccgac cgacgccgaa ctggtcaaac agtgggccac
     1921 gacctggacc aaccagcttg gatttgaact ggtcaacagt ggcgatccct ggatgcacct
     1981 tcaactcatc cgccaaacca cccagaaata tcagattcca atgcctgaca ttggccagta
     2041 tgtgcagatg caacggcgag gtgacacccg atttcaggat gacgttgatc tgttttcgat
     2101 caccacgggc ggcctggcca tccaggaatc actccagtac aaacgattgc tcaatatctc
     2161 agaaaatcgg ggcggtcaga ccgtgccgat taccaaactg gaaggcccgg cggtcaaatc
     2221 gcatccattt gagaaaatgc tcgaaggtcg ccagccagtc cagtttgaac tcgccaaact
     2281 ggtgccgcat gaccagtatt atgtccattt ttcaaccatc aaaagcgaga ttgagctgtc
     2341 tgacctgctc gaccagtggg gtacaacgct cctgcaatcc gttgaggtga cgtcgcatga
     2401 ccggcaggta aaagaacgcc tgttgaccca gctctgtctc gaactctcca gcctgacccg
     2461 gttgtttggc agtttcgtgg tggacgacct ggctctgacc ggaaatgacc cgtttgtgac
     2521 cgacggcaat gatgtgagcg tcatctttaa cgtcaaaaac ggtccgattt tcgatcaatc
     2581 aatggcggcc caccgggctg aggccaaaaa acagcgtccg gatgcggtcg agtcaaccgc
     2641 ccgctacggc acctttgaaa ttggcacgtt gaccacacca gaccggacga tttcttccta
     2701 ctggacggca gttgacagct accgcatcta ttcaaattcg cggtcggcac tggaacgcat
     2761 cctggatgca gcgcagggta aacgaccatc actcaaagcg gcagccgaat ttcgctatat
     2821 gcgcacgatc tttccgggca ccgcaagcga gga
//
LOCUS       DAOQDO010000630         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28813_length_2852_cov_2.686092, whole genome shotgun sequence.
ACCESSION   DAOQDO010000630 DAOQDO010000000
VERSION     DAOQDO010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28813_length_2852_cov_2.686092"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKZ53_10885"
     CDS             <1..465
                     /locus_tag="PKZ53_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40985.1"
                     /translation="DYEKRFTLLEEKLLKESGELRTEVKKRLDGLEAYVKKELESLND
                     RLKAEHDERTTSTKAIIQELRDFSQNTEKRISQLDEFTTKTQRELRHEMLNQHKELSE
                     DIRQKAEALSGALEKAFKELRGDKTDRFALAALFTEMAMRLNNQFKLPGSEG"
     gene            472..2382
                     /locus_tag="PKZ53_10890"
     CDS             472..2382
                     /locus_tag="PKZ53_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ40986.1"
                     /translation="MAGSSGESHFQSDSSPLPGSSSDDLETLRRLLFQTSSETTAAPD
                     DLEALRALLLTMDQTSAPSELTELRHLLMGPEQARLDQLQHQLEDPALLAETVGSVLP
                     EAILLRSTADKRLLKALSPMIAEVLQVSVKKNPKFLADILFPILGPLIRKSISAALSS
                     MIQTLNQALEHSFSAKGLKWRFEAWSTGRSFAEVVLVNTLVYRVEQLFLIHKQTGLLL
                     CHVANTDTAPRDPDLVSGMLTAIQDFAQDSFTVEQGAQLETLQVGDLNVWIEQGPKAV
                     LAAVIRGNAPHTIRNTFQDALETIHLEQSQTFDEFQGDVSFFENCRPTLEACLLAQFG
                     DHKSSSTTLPWPVLAAAGVLLVLAAGWGGYEFWQYRKWNRYLDRLRAEPGIVVISTGT
                     KEGKFTVTGLRDPLAIQPEALLKETGLGPHQVLAQWEAYLASSPPFVLHRATKLLNPP
                     STVTLEFSNGTLVATGTASHRWIQDANRFGSVLPGVVGIQTSRVIDADLVQFDTLKAK
                     IEHTELFFGEGTTLLIPGQEQLLAELTRNLQQLNTTAMTSGFMVSLEIFGHTDQTGTD
                     ELNQRLSLERADVIRLHLIQQGVITTQLASTGAGTSLSQSVEATPQPNEAHRKVTFRV
                     KFEEISLSPRSK"
     gene            2447..>2852
                     /locus_tag="PKZ53_10895"
     CDS             2447..>2852
                     /locus_tag="PKZ53_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HNJ40987.1"
                     /translation="MIQKKVCLLGAYAVGKTSLVARFVKSIFSEKYHTTVGVKIDKKT
                     IQIDQQTLSCILWDLHGEDEFQKVRMSYLRGSSGYLLVVDGTRRTTLQTAIELQRTVE
                     EAVGKVPFILVLNKSDLIYEWELDESELSELTA"
BASE COUNT          726 a          763 c          715 g          648 t
ORIGIN      
        1 gattatgaaa agcgctttac cctgcttgaa gagaaactgc tcaaggaatc gggtgagttg
       61 cgaaccgagg tcaaaaaacg gcttgatgga ctggaagcct atgtcaagaa agagcttgaa
      121 tcgctcaatg atcgcttaaa ggccgaacat gacgagcgaa ccacatcaac caaggctatt
      181 attcaggaat tgcgcgactt tagccagaac actgaaaaac gcatctctca gcttgatgaa
      241 ttcaccacca aaacccagcg tgaactccgg cacgaaatgc tcaatcaaca caaagagctg
      301 tcggaagaca tccgccaaaa agccgaagcc ctctctggcg cgctggagaa agcatttaaa
      361 gaacttcgcg gcgataaaac cgaccgcttt gccctggcag cactctttac cgaaatggca
      421 atgcggctca acaatcagtt taaactgcca ggttcggaag gataaatcgt catggctggg
      481 tcatctggcg aatcccactt tcagtcagat tcttccccgc tgcctggttc atcatccgac
      541 gacctggaga cattgcgccg cctgctcttc cagacctcat ctgaaaccac ggcggcgccc
      601 gacgacctgg aagccttgcg ggcgctgttg ctcacaatgg accagacgtc ggcacccagc
      661 gaactcaccg aattacgaca tctcttgatg ggaccggaac aggcccggct cgatcaactt
      721 cagcaccaac tcgaagaccc cgcactgctg gctgaaaccg tgggtagcgt gctgcccgaa
      781 gcaattttgt tgcgatcaac ggctgacaaa cggctcctca aagccctgtc ccccatgatt
      841 gctgaagtat tgcaggtttc tgtcaaaaaa aaccctaagt tcctggccga tatcctgttt
      901 ccaatcctgg ggccgctgat tcgcaagtca atttcagccg ccctgagcag catgattcag
      961 accttgaatc aggcgctgga gcatagtttt tcggccaaag gactcaagtg gcgctttgaa
     1021 gcctggtcca ccggtcgctc gtttgccgaa gtggttctgg tcaacacact cgtctatcgg
     1081 gtggaacaac tgtttctgat ccacaagcaa actggattgt tgttgtgtca cgtcgccaat
     1141 accgacacag caccacgtga cccggatctg gtttcgggta tgctcacggc cattcaggat
     1201 tttgcgcagg attcgtttac ggttgagcaa ggggcccaac ttgaaacctt gcaggtcggt
     1261 gatttgaacg tctggattga gcagggcccc aaagccgtac tggccgccgt gattcgcgga
     1321 aatgcaccgc acacaatccg caataccttc caggatgccc tggaaaccat tcatcttgaa
     1381 caaagtcaga cgtttgatga atttcaaggt gacgtttcgt tctttgaaaa ctgccgacca
     1441 acccttgaag cctgtttgct ggctcaattt ggtgaccaca agagttcatc caccacatta
     1501 ccctggccag ttctggcagc ggcaggagtc ctgctggtcc tggctgccgg gtggggcggg
     1561 tatgaattct ggcaatatcg aaaatggaac cgctatctgg accgactgcg cgccgagcca
     1621 ggaattgtgg tgatctcaac cggaaccaaa gagggcaaat ttacggtcac tggcctgcgg
     1681 gatccgctcg ccatccagcc agaagccctg ctcaaggaaa ctggattggg tccgcaccag
     1741 gttctggccc agtgggaagc gtacctggcg agttccccgc cttttgtgct gcatcgcgcc
     1801 accaaattgt taaatccgcc ctcaacggtc accctggaat tttccaacgg cacgcttgtg
     1861 gcaaccggaa cagcgtccca tcggtggatt caggatgcca atcggtttgg gtctgttctg
     1921 ccgggcgtgg tggggatcca aaccagtcgg gtcattgacg ctgatctggt tcaatttgac
     1981 acgttgaaag ccaaaatcga acataccgaa ctcttttttg gtgaaggcac cacccttttg
     2041 attcccggtc aggaacaact ccttgccgaa ctcacccgga atctgcaaca actcaatacc
     2101 acagcgatga cttcggggtt tatggtctcg ctcgagattt ttggccacac cgaccaaact
     2161 ggcacggatg aactcaatca aagacttagc ctggaacgcg cagatgttat tcggttgcac
     2221 ctgattcaac agggcgtgat tacgactcaa ctggcttcaa ccggagctgg aaccagtctg
     2281 agccagtcgg ttgaagccac accgcaaccc aatgaagccc atcgtaaagt gacgtttcga
     2341 gtcaaatttg aagagatcag cctgtcacca cggtcgaaat aactatggac acacgaagaa
     2401 gggacgattg cgctcattca ttttgaggaa cacgggattt ttccaaatga tccagaaaaa
     2461 agtctgcctg cttggcgctt atgcagtggg aaaaaccagt ttggtggcac gttttgtgaa
     2521 aagcattttc tcagaaaagt accacaccac cgtcggtgtc aagattgaca aaaaaacaat
     2581 tcagattgat cagcaaaccc tttcctgtat tttgtgggat ctgcacggcg aagatgaatt
     2641 tcaaaaagtc cgcatgtcgt atttgcgtgg ctcatccggg tatttgctgg tggttgatgg
     2701 cacccgacgt accacgcttc aaacggcaat tgagttgcaa cgcaccgtag aggaagccgt
     2761 tgggaaggtt ccctttatcc tggtgttaaa caaatctgac ctgatttatg aatgggaact
     2821 cgatgaatca gaactcagtg aattaacggc tc
//
LOCUS       DAOQDO010000631         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28938_length_2844_cov_2.200430, whole genome shotgun sequence.
ACCESSION   DAOQDO010000631 DAOQDO010000000
VERSION     DAOQDO010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28938_length_2844_cov_2.200430"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKZ53_10900"
     CDS             complement(<1..382)
                     /locus_tag="PKZ53_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40988.1"
                     /translation="MSKNISSKSTKNEILDVLNEVTEEKEQLEQNLSEAEGAVEQLTK
                     ENKSLKSQLEALRKEKKSTEPTPSTPQEPPARGDRKPMPEIQTGATIPQIINDLTSLK
                     ARFGTAVSELSTRLTSEVSTLQQLR"
     gene            complement(462..1460)
                     /locus_tag="PKZ53_10905"
     CDS             complement(462..1460)
                     /locus_tag="PKZ53_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40989.1"
                     /translation="MVANGTPADNKTQIVKSFQQLLESRKRAASQVATKEIAAEKQKD
                     AAILAQASTYTIETTVKGLADLQLDFGHTIDAVATKLTTETGKLDELRRAIEVERENL
                     VDLNNTQIAADALDILTQEHREQLRAFEEDQARRTTELETEISNTRTNWKKEQESFDR
                     LAQEQAELIQKERQQAEADYQYEVENRRKIEANNYEDRKRKLEIELTETEKEKEKRWT
                     ERETILAERQPQIEEHKAKVETMPAELEKAIKTAREDAIKDTHEAEKQKALLLEKETE
                     ATHKIFDLKIQSLEATVTHQTEEIAHLTTQLQAALKQVQELAMRAIEGTTKNKLSA"
     gene            complement(1686..2123)
                     /locus_tag="PKZ53_10910"
     CDS             complement(1686..2123)
                     /locus_tag="PKZ53_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNJ40990.1"
                     /translation="MKVIAIALLTTSLWVSQSALAQEKSEPQPTTKPAAESQKNPEIP
                     EGMTVYYMGLLSRGPKWTPEKTAETAKIQEQHMAHIRSMGESGKLVIAGPFLDGGQLR
                     GIFVFKVSTLEEARAMAEADPAVKAGRLQVEIHPWMVDKGILP"
     gene            complement(2162..>2844)
                     /locus_tag="PKZ53_10915"
     CDS             complement(2162..>2844)
                     /locus_tag="PKZ53_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HNJ40991.1"
                     /translation="AFPPGSKEPGLHAEDRMKFRVVFCAILVFIGAELMSANMLAQEK
                     QAPSNSPAPSQAQMEEMMKKYQELSMPGPAHERFKSLVGTWNVTNRAWMDPKGEPVIS
                     KGTCEYKLILGGRYLVQEFHSTFMGQPFHGMALVGYDNFRKQYTSTWVDSMSTAIMVS
                     TGTGDASGKVFTYTGTMDDPVLGIKDKVCKSIERIIDQDTHVFEMYDTIPDQGEFKMM
                     EITYTRKK"
BASE COUNT          571 a          625 c          730 g          918 t
ORIGIN      
        1 tgcggagttg ctgcagggtt gaaacttcac tcgtcaggcg ggttgaaagc tcgctgacgg
       61 cagtgccaaa acgggctttt aaactcgtta aatcattgat gatttgggga atcgtcgctc
      121 cagtttgaat ttcgggcata ggttttcggt ctccgcgggc gggtggctcc tgaggggttg
      181 agggtgttgg ttcagttgat tttttctctt tgcgcagggc ttcgagttgg gatttgaggg
      241 atttattttc tttggtcaat tgctcaacgg caccttcggc ttcgctgagg ttttgctcaa
      301 gctgctcttt ttcctcagtc acttcgttga ggacatccag aatttcattt ttggtgcttt
      361 ttgaagagat atttttgctc atggcacgat cctttcggtg aatgcggatg tcggatgtga
      421 tgtgacgtca acgacatcag ttccgcttga gtggaggact ctcaggctga gagtttattt
      481 ttggttgtgc cttcaattgc cctcatggcc agttcctgaa cctgtttcaa tgcggcctgg
      541 agctgagtgg tcaggtgtgc aatctcttca gtttggtgag tgacggttgc ttcaagcgac
      601 tgaattttaa gatcaaaaat cttgtgggtc gcttcggttt ctttttcaag caggagcgct
      661 ttttgttttt cggcttcgtg ggtgtcttta atggcatctt cacgagcggt tttgattgct
      721 ttttcaagct cggcgggcat ggtttcgact ttggctttgt gctcttcgat ttgtggctgg
      781 cgttcagcca ggatggtttc gcgttcggtc cagcgttttt ctttttcctt ttcagtttcg
      841 gtcagttcaa tttcgagttt gcgtttgcgg tcctcatagt tgttggcttc gattttacgg
      901 cgattttcaa cttcgtactg ataatcggcc tcagcctgct ggcgttcttt ttgaatcaat
      961 tcggcttgtt cctgggccag tcggtcaaaa ctctcctgtt cttttttcca gttggtgcga
     1021 gtgtttgaaa tttcagtttc cagttcagtg gtgcggcgag cctggtcttc ttcaaatgct
     1081 cggagttgtt cgcggtgttc ctgggtcaaa atatcaagtg catcagcggc aatttgtgtg
     1141 ttgttgaggt caaccaggtt ttcgcgttcg acctcaatgg cccgacgcag ttcatcgagc
     1201 tttccagttt ccgtggtcaa tttagtggca accgcatcaa ttgtatggcc aaaatccagt
     1261 tgcaggtcgg ccagtccctt gactgtggtt tcaatggtgt aagtcgaagc ctgcgccaaa
     1321 attgcagcat ctttttgttt ttccgctgca atttctttcg tggcgacctg ggaagccgcc
     1381 cgttttcgtg attccaaaag ttgctggaag gatttaacga tttgggtttt attgtctgct
     1441 ggggtaccgt tcgcaaccat gatggatgct tctccttttg gaaaatattg attgttgagg
     1501 gtttatgaac cagtgccttt cttgtttcaa ggcaaaaaaa tatcctttgc acatcagtgt
     1561 gttgtttgaa ctgagacttt gttgaaaatc aaaaaatcag tcaagtcaca aactcaggcg
     1621 caaaggataa gagttgattc ttcaggagtc gaagagtcgt tatggaaatg ggtttggatc
     1681 accggtcacg gcaaaatgcc tttatcaacc atccaggggt ggatttcgac ctgcaatcgc
     1741 ccggctttga ctgccggatc agcttcagcc atggccctgg cttcttctaa ggtgctgact
     1801 ttaaagacaa aaatcccacg taattgacca ccatccagaa atggacccgc aatgacgagc
     1861 ttgccggatt cacccatgct gcgaatatga gccatgtgtt gctcctgaat tttggcagtt
     1921 tcggctgttt tttcgggtgt ccatttgggg ccacgactga gaagccccat ataatacact
     1981 gtcattcctt cagggatctc cgggtttttt tgactttcgg cggctggttt ggtggttggc
     2041 tgtggttctg atttttcctg agccagagcg gattggctaa cccagaggct ggttgtaagc
     2101 aatgcaattg caatcacttt catacttaat attccttgaa acgaagtgtg tttccctggc
     2161 tttatttttt ccgggtgtag gtaatctcca tcattttgaa ttcaccctgg tctgggattg
     2221 tgtcatacat ttcaaagaca tgggtatcct ggtcaatgat ccgctcaatg gatttgcaga
     2281 ctttgtcttt aatgcccaaa accgggtcat ccattgtccc agtgtaggta aacactttcc
     2341 cggatgcatc gcctgttccg gtgctgacca taatggccgt actcatcgaa tcaacccagg
     2401 ttgaggtgta ttgcttgcgg aaattgtcat atcccaccag ggccattcca tgaaatggct
     2461 ggcccataaa ggtactatga aattcctgaa ccagataccg gccacccaga atcaatttat
     2521 attcacacgt ccctttggag atgactggtt cgccttttgg atccatccag gcccggttgg
     2581 tgacattcca ggttccaacc agggatttga atcgctcatg agccgggcct ggcatggaga
     2641 gttcctggta ttttttcatc atttcttcca tttgggcttg agatggggct ggggaatttg
     2701 atggggcttg tttttcctgg gcaagcatgt tggctgacat caattcagcg ccgataaaca
     2761 ccaagatagc gcaaaataca actcgaaatt tcatcctatc ttcggcgtgg agacccggtt
     2821 ctttagagcc gggaggaaac gccg
//
LOCUS       DAOQDO010000632         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_28948_length_2843_cov_2.492468, whole genome shotgun sequence.
ACCESSION   DAOQDO010000632 DAOQDO010000000
VERSION     DAOQDO010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28948_length_2843_cov_2.492468"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PKZ53_10920"
     CDS             complement(<1..1288)
                     /locus_tag="PKZ53_10920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNJ40992.1"
                     /translation="MSGFTSVTNGQATDYHHIPRLDFATFRDGLLAGVRQGHRVASLF
                     GKAGEESPGIELVLVLADDQQNCLFIGKTFIPDNQFPSLTPDCPQVHLFEREIAEQFN
                     LQPIGHPWLKPVRFAPPLNDSAGGISPIQPPTIGQMDFYRVLGEEIHEVAVGPVHAGV
                     IEPGHFRFQCHGEKVFHLEISLGYQHRGVESALIGGPHPLTLRQIETIAGDTTLGHSW
                     AYCQNLEALSQTRVTAREQVIRAIGLELERLANHTGDLGALAGDVGYLPTQSFCGRIR
                     GEFLNMTATLCGNRFGRSLLRPGGVRFDLEPGQIETLIDQLDAAETDLISAVELLWET
                     PSVMGRFENTGVVTRDQGLQLGFVGPTARACGIERDVRTDHPTGVYQFVNLPVAMTHY
                     GDVNSRAYVRWLEIRRSLRFIRERLTAMPEPRQTSTP"
     gene            complement(1285..2688)
                     /locus_tag="PKZ53_10925"
     CDS             complement(1285..2688)
                     /locus_tag="PKZ53_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ40993.1"
                     /translation="MAAGIAYVIRSNWARPMLLPVVALAHLVMVVLALDQETIQIETG
                     WLQLDPLGKIILLSISILFFCCSLYAPGYLRLRQDRPNQVFCACLLILLGMMSLVAVT
                     HHLGLMWVAIEATSLASAPLLFFNRTPHSLEAAWKYLLICSVGIALALLGSFFLAYSS
                     LYGGLESTLLFDDLVKSAPQLSKPWLHAAFVTLLVGYGTKMGLAPMHTWKPDAYGEAP
                     GIVGALLAGGVTSCAFIAIFRVTTILNAAQESRFASKILIGIGLLSMAVAAVFLVRQK
                     DYKRMLAYSSIEHMGILVLGIGLGGPGIFGALLHLLNNAFAKGVLFLSSGNIHRAYGS
                     KSTDQVRGALVRLPASGALFLGGFIAITGSPPFGPFMSEFIIVRQAFQTGRFGVVGLV
                     LVLLFAIFIGMGNTVLAMVQGTPEPTGSPGNFHDTPQTVIPIILLFLLVLGLGIFLPP
                     SLAHLLTEAAVYLEGHV"
BASE COUNT          775 a          763 c          739 g          566 t
ORIGIN      
        1 caggggtgct ggtttggcgc ggttcgggca tagcggtgag tcgttcccgg ataaagcgca
       61 gggaccgacg gatttcaagc catcggacgt aggcccgtga attcacatct ccataatgcg
      121 tcatggccac cggcaaattg acaaactgat aaactccggt cggatgatca gtccgcacat
      181 cgcgctcgat gccacaggcg cgcgcggttg gaccgacaaa ccccaactga aggccctggt
      241 cgcgagtgac cacacctgta ttttcaaagc gacccatcac cgatggcgtt tcccagagca
      301 attcaacggc tgaaatcagg tcagtttcag ccgcgtcaag ctggtcaatc aacgtttcaa
      361 tctgacctgg ttcaagatca aagcgaactc cccctggacg cagcagactt cttccaaacc
      421 ggtttccaca cagcgttgcc gtcatattca gaaattcccc ccgaatccgc ccgcaaaatg
      481 attgggtggg cagatacccg acatctccgg ccagtgctcc caggtcgccg gtgtggttcg
      541 ccaatcgttc aagctcaagc ccaatcgccc gaatgacctg ttcgcgggcg gtgactctgg
      601 tttgggacag ggcttcaagg ttctggcaat aggcccaact gtggcccagc gtggtgtctc
      661 ccgcaatggt ttcgatctgg cgcagggtca atgggtgcgg cccgccaatc agcgctgatt
      721 caaccccccg gtgttgatac ccaagtgaaa tttccaggtg aaacactttt tcgccgtgac
      781 attgaaaccg aaaatgcccc ggttcaatga caccagcgtg gaccggacca acggcgacct
      841 cgtggatttc ttctccaaga acccgataaa agtccatttg tccaatggtt ggtggttgga
      901 tggggcttat tccaccagca gaatcgttca acggcggtgc aaatcgaact ggtttcagcc
      961 acggatgtcc aatcggctgg aggttgaact gttcggcaat ttctcgttca aacaggtgaa
     1021 cctgggggca atcgggcgtc aaggatggaa actggttgtc tggaatgaac gtcttcccga
     1081 taaagaggca attctgctga tcatcagcca gcaccagaac cagttcgatg ccgggagatt
     1141 cttcgccagc ttttccaaac aacgacgcga cgcgatgccc ttgccgaaca cctgccagca
     1201 gcccatcacg aaaggttgca aagtccagcc gtggaatatg gtgataatcg gttgcctgac
     1261 cgttggttac ggaggtgaac ccgctcatac gtggccctcc agatagactg cggcttcggt
     1321 gagtaaatgg gccagcgatg gcgggagaaa tatcccaagt cctaaaacca gcaaaaacaa
     1381 taagataatc ggaattaccg tttgcggtgt atcatggaaa ttccctggtg aaccagttgg
     1441 ctcaggtgtg ccttgaacca tcgccaaaac ggtgtttccc atcccgataa aaatggcgaa
     1501 caagagcacc agcaccagtc cgactacgcc aaatcgcccg gtttgaaagg cttgtcggac
     1561 aatgatgaat tcactcataa agggcccaaa cggcggcgaa cccgtgatgg caataaatcc
     1621 gcccaaaaat aaggcacctg aagctggaag cctcaccaat gcaccacgaa cctgatcggt
     1681 tgatttgctg ccataggccc gatggatatt gccggatgaa agaaataaca cccctttcgc
     1741 aaaggcatta tttaacaaat ggaggagcgc cccgaatatt cctggtccgc caagtccgat
     1801 tccaagcacc agaatgccca tatgttcaat gctcgaatag gccagcattc gcttatagtc
     1861 tttctggcgt accagaaaca cggcggcaac cgccattgaa agaaggccaa ttccaatcaa
     1921 gattttgctg gcgaagcgtg attcctgagc ggcatttaaa atcgtcgtga cccggaaaat
     1981 ggcaataaag gcacaactgg taacacctcc ggccagtaag gcgccgacaa ttccgggggc
     2041 ttcaccgtag gcatccggtt tccaggtatg cattggcgcc agacccattt tggtgccata
     2101 cccaaccaga agtgtgacaa acgccgcgtg caaccagggt tttgaaagct gaggcgcaga
     2161 tttcaccaga tcgtcaaaca acagggttga ttccaatccg ccgtacagtg aggaataagc
     2221 caggaaaaaa gacccaagca gcgccagggc aatgccgacc gaacaaatca gaagatattt
     2281 ccaggcagct tcgagcgagt gcggggttcg attaaaaaat agcaagggag cactggccag
     2341 cgacgtggct tcaatggcaa cccacatcaa ccccaggtga tgggtaaccg ccacgagcga
     2401 catcattccc agcaaaatga gcaggcaggc acaaaacacc tgattcggtc ggtcctgtcg
     2461 gagtcgaagg tagcccggcg catagagcga acaacaaaaa aacaggatgc tgattgagag
     2521 cagaataatt tttccgagtg ggtccaactg caaccagcca gtttcaatct gaatcgtctc
     2581 ctgatctaaa gcgagaacca ccatcaccag atgggccaat gcaacaacgg gtaacagcat
     2641 tggccgggcc caatttgacc ggatgacata ggcaatgcct gccgccatca gtggaattgt
     2701 aaaaataaag atgagtaaca cggtaatacc ttttaggggt cagggttcag ggtttggggt
     2761 ttggggacag acaaagacaa aaaggacgaa aaggacgaaa aggacataaa ttcgaaaacc
     2821 ctgaaccctg aaccctgaac cct
//
LOCUS       DAOQDO010000633         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29045_length_2838_cov_2.523895, whole genome shotgun sequence.
ACCESSION   DAOQDO010000633 DAOQDO010000000
VERSION     DAOQDO010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29045_length_2838_cov_2.523895"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="PKZ53_10930"
     CDS             <1..1770
                     /locus_tag="PKZ53_10930"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNJ40994.1"
                     /translation="AAWRHSPIGAGRADLPGGTMQSLTNWLQYRRRAIYWEAEGVESS
                     VALSIADGFAFVVNGKTDGHVRADAGTQILSGLLGAMVHPHPKKSLVVGLGTGCTAGW
                     LATVPTMERVDVIELEPAILEVARACAPVNMNAMDNPKVKIHIGDAREVMITTPESYD
                     LIFSEPSNPYRAGISSLFTRECYLGVAQRLNENGIFMQWVQGYEVDSKTIRTTYATLA
                     SVFPTVETWQTQDGDLLLFASKKPIVYDLPAIRTRIKEEPYKTSLMQVWRVTSVEGLF
                     SHYLANARFAQAVAAAVGPNLNTDDRNNLEFGFARNVGSQDNFRMIDLIHTATRRKEC
                     FPLVTGGELNWKLVEDMHMTRLTNENKPPESLPSHTPEQKIRLLAQSHYVKQEFKEAL
                     AMWRLQPRYPQDLTELTMLAECLADAGDEGAFEYLNLLKQLQPVEAQAILARLRWRQG
                     RLDEATAALESVFQVYHSNPWPSLWLMTRTVGVAESVAKSDPGKQFARRLYTALKTPF
                     CACLINETRLSAQLEIARYVEDKALLVETFTAYEPNFPWQASYLVERYQLYRELNHPL
                     AAQAEHDIHQFLRYEASPFDEGL"
     gene            complement(1788..>2838)
                     /locus_tag="PKZ53_10935"
     CDS             complement(1788..>2838)
                     /locus_tag="PKZ53_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ40995.1"
                     /translation="VFRLGLLISGIVACVGLLGGFYSNWLPDQALVLCAVHFGLLSVL
                     WLSCGILTVCTPTPAIAGVFTISVGLYLVLVLFTSVPVLTAQTASIVVAGLSTAWMAR
                     RALPSLNAQEPSAAFPDPVVLGYLLVPYFCVGCGYFAFLFTDRWLAGTVVAFGFGTPF
                     VAHPEYQLGIDLALLLFFLLVGFVEVCAFRFTQGIFEQAAQMDLLQGNFHKQVYTQYL
                     RFLFGLASLVAIILILVWNLPESTWVLLTASAAQAPIVRSIALLGTLSYGCLALALFQ
                     GLILFSFSQPSAVVWSLSWGLAINLLTSYFLSNLLGFEFTVCGVLCGAVIWMWVGIQS
                     VHKLIRELEYSYFAM"
BASE COUNT          802 a          706 c          697 g          633 t
ORIGIN      
        1 gcagcctggc ggcatagccc tattggtgct ggtcgagctg atctgcctgg cggcactatg
       61 cagagcttaa caaactggtt gcagtatcgg cggcgagcaa tttattggga agctgaaggt
      121 gtggaaagta gtgtcgcctt atccattgcc gatggttttg ccttcgttgt gaatgggaag
      181 accgatggtc atgtccgagc ggatgccgga acccagattt tgagtggatt attgggggca
      241 atggttcatc cacaccctaa aaaatcactg gttgttgggc ttggtaccgg gtgtactgct
      301 ggctggttag ctactgttcc aaccatggaa cgggttgatg tcattgaact ggagcctgcc
      361 attttggaag ttgcccgggc gtgcgctccc gtaaatatga atgccatgga caacccaaaa
      421 gtcaaaatcc atatcggtga tgcgcgtgag gtgatgatca ccacaccaga gtcctatgac
      481 ctgattttct cagagccttc aaatccatat cgggctggaa tctcaagcct gtttacccgt
      541 gaatgttatc tgggggttgc gcaacggctc aacgaaaatg ggatttttat gcagtgggtg
      601 caaggctacg aagttgattc caaaactatt cgaacaacct atgccacact cgccagcgtg
      661 ttcccaacgg ttgaaacctg gcaaactcag gatggagatt tgctgctctt tgcgtcaaag
      721 aaaccaattg tgtatgacct gcccgccatt cgtacccgaa tcaaagaaga gccctataaa
      781 acctcactca tgcaggtttg gcgagttaca agtgtggaag gactgttcag tcattattta
      841 gcgaatgccc ggtttgctca ggccgtagcc gctgcagtcg gtccaaatct gaatactgat
      901 gatcgcaaca accttgaatt cggatttgct cgcaatgttg gaagccagga taattttagg
      961 atgattgact tgattcacac tgcaacccga cgaaaggaat gtttcccact ggtaacagga
     1021 ggtgaattaa attggaagct ggttgaggat atgcatatga cccggcttac caatgaaaat
     1081 aaaccccctg aaagtctgcc gtcccacacc cctgagcaaa aaattcggtt gcttgctcag
     1141 tcccactacg tcaaacaaga gttcaaagag gcgctcgcaa tgtggcgact tcaaccaaga
     1201 tatccacagg atctcaccga gttgaccatg ctggctgagt gtttggccga tgctggtgat
     1261 gagggggctt ttgagtacct caatctattg aaacaactcc aacccgttga agcccaggcc
     1321 attctggcaa gacttcgctg gcgacaggga agacttgatg aagcgactgc ggcacttgaa
     1381 agtgtttttc aggtatatca ttccaatccc tggccgtcac tttggttaat gacccggacg
     1441 gtaggagtgg cagaatcagt cgccaaatct gaccctggta aacagttcgc acgccgactc
     1501 tacactgcct tgaaaacacc tttttgcgcc tgcctcatta atgaaacccg attatccgca
     1561 caattagaaa ttgcacgtta tgttgaagac aaagcgctgc tggtggagac atttaccgct
     1621 tatgagccaa atttcccatg gcaggcctca tatcttgtcg aacggtatca actctatcgt
     1681 gaattgaatc atccactggc tgctcaggct gaacacgata tccatcaatt tttgcggtat
     1741 gaagccagtc cctttgatga aggactgtaa ggtgatcatt gggtgcttta catggcaaag
     1801 taagaatact caagttctcg aatgagctta tgtactgatt gaatgcccac ccacatccag
     1861 atcacagcac cgcaaagaac accacatact gtaaactcga atccaagaag attgctcaga
     1921 aaataacttg taagaagatt gatggctaac ccccaactca aagaccatac cacggctgac
     1981 ggttgtgaaa aactaaacag gattaagcct tgaaataacg ccagtgccag acagccatag
     2041 ctcaatgttc caagcagggc aatactgcgc acaattgggg cttgagccgc cgaagccgtg
     2101 agcaagaccc aggttgactc tgggagattc cacaccagga tgaggatgat ggcgacgaga
     2161 ctggctagcc caaataaaaa ccgtaagtac tgtgtataaa cctgtttgtg gaaatttcct
     2221 tggagcaggt ccatctgggc ggcctgctcg aaaattccct gggtaaatcg gaaggcacag
     2281 acttcgacaa atccaaccag tagaaagaac agcaggagag ccagatcaat cccaagttga
     2341 tactcggggt gcgcgacaaa aggcgtccca aatccaaaag ccacaaccgt tccagccagc
     2401 cagcggtctg taaacaggaa ggcaaaatac ccgcacccaa cacaaaaata gggtaccagt
     2461 aaatacccaa gcactaccgg gtctggaaag gcggctgaag gttcctgtgc attcagagaa
     2521 ggtaaggccc ggcgtgccat ccaggccgta ctcaggccag ccaccacaat cgaggctgtt
     2581 tgagccgtca acaccggtac tgaagtaaaa aggaccaaca ccaggtacag accgactgag
     2641 atagtaaata ccccggcaat ggctggcgtt ggcgtacaaa ccgtcagaat cccacagctt
     2701 aaccacaaaa cactgagtag cccaaagtga accgcgcaca ataccagcgc ctgatccggt
     2761 agccagtttg aataaaatcc gccgagtaac ccaacacagg ccacaattcc tgaaatcaga
     2821 agccccaggc gaaagact
//
LOCUS       DAOQDO010000634         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29061_length_2837_cov_3.056075, whole genome shotgun sequence.
ACCESSION   DAOQDO010000634 DAOQDO010000000
VERSION     DAOQDO010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29061_length_2837_cov_3.056075"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..222)
                     /locus_tag="PKZ53_10940"
     CDS             complement(58..222)
                     /locus_tag="PKZ53_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HNJ40996.1"
                     /translation="MANCIECDAPVTVPVDVLDGEIIPCGDCGAELEVLALEPLSVGL
                     APAVQEDWGE"
     gene            complement(499..1758)
                     /locus_tag="PKZ53_10945"
     CDS             complement(499..1758)
                     /locus_tag="PKZ53_10945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNJ40997.1"
                     /translation="MRRYFLFAFLNLVLVGTAIVQIIDQPQVAVYGKIYLALGDLEQA
                     AIEKSSQAVNTRIKELKTLDLMQNVHSEVYQAADKASGLTRKAQEADFEPLVKICRTE
                     IDRLSTLNAQEASHRVHQKQPIWIVAALLCVINSVSFTYVGLRHRRESQLRSELELEL
                     SDKESALAQAMFHNRDRIITMLQQGRENRKLMTLLVKEREEALLDAMTGIYGVYSKKG
                     MSKFYQVIASQLGRQPIGVAMLDIDFFKRVNDTLGHPAGDVVIKALAKILSKNIRTQD
                     DFVIRFGGEEFVVVFPNVDPAELDTIIGRIQSEVRSTKIYVKDLNGQEVLVKSTIDPD
                     CSITISCGYAQASESKQYQYLITHLREHPPTSLEGDVTPETWELLHDFTEELKTLVDK
                     RLYVAKQSGRNQTCGPKTRVTGSLISG"
     gene            complement(1813..>2837)
                     /locus_tag="PKZ53_10950"
     CDS             complement(1813..>2837)
                     /locus_tag="PKZ53_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNJ40998.1"
                     /translation="TCDEEGNEIKGRYRVHVYRSEPVSPLGSGLCLSFRRIWPVLPTL
                     KQLRFSPAAGSLVYDLMNGTISSGLILVVGSTGSGKSTTVGAMLEAVNDRLPVNITLF
                     EDPPEYRFTNRRARFRQIYVGRDVASYTEGAKHALRQDPDIIVVGEIRDEDMANQALA
                     MAKTGHLVIGTMHSSTNAPAALLKFLQMTGDDFFSISNHLQAIIAQKILPPKHRYSLD
                     ENGVQQATLPVLIQEVMRPTKNKQIQALLRTQSDDPYAYYLAIGTENTETLILEESFG
                     TALKNAVEQSLIYNSMVLKAAERDEDAAIGGSYRKSGRLSGKHRAPEFLRNRNQKTGP
                     LDTSDQ"
BASE COUNT          663 a          734 c          714 g          726 t
ORIGIN      
        1 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc cggctcttca
       61 ctccccccaa tcttcctgga cggcgggtgc cagaccaacg gaaagtggtt ctaatgcaag
      121 gacttccagc tcagccccgc aatcgccaca ggggataatt tcgccatcaa ggacatcaac
      181 tggaacggtt acgggtgcgt cacattcgat acaattcgcc atggtgttcg agcctccaaa
      241 tcagggtatg ggtgggtgga aaaagaaaac gcccgccaga ccggtttgtg gtctggcggg
      301 cgtttgaggg agcaacaaca caaaaaaaca ggcaaacgca actatccaga cctcttgtcc
      361 ggtttcttca cgcgcttgct ttgcttcttc aggtttgtaa cttgcatgag ttctattttg
      421 aaaatggtag aaaatgaagt aatgacgtgg gcatcctagc ggtattacaa aaaggtgtca
      481 aatacttttt tcggtgcctt atccagaaat aagcgaaccc gtcacccgtg ttttaggccc
      541 acaggtttgg tttcgccctg actgtttggc aacatacagc cgtttatcca ccagtgtctt
      601 taactcttcg gtaaagtcgt gaaggagttc ccaggtttct ggggtgacgt caccttcaag
      661 cgaggttggc gggtgctcgc gaaggtgagt gatcaggtac tggtactgct ttgactctga
      721 agcctgagca tagccgcagg aaatggtgat ggagcagtct gggtcaattg tgcttttgac
      781 cagaacctcc tgaccattta agtccttgac atagattttg gtgctacgga cttcagattg
      841 gattcgccca atgatggtat caagttcggc tgggtccacg ttaggaaata ccacaacaaa
      901 ttcttcccca ccaaaccgga tcacaaaatc atcctgggtc cgaatatttt ttgagaggat
      961 tttcgccagc gctttaatca cgacatctcc ggctggatga cccagcgtgt cattgacccg
     1021 cttaaaaaaa tcaatatcca gcatggcgac cccaattggc tggcgaccaa gctggctggc
     1081 aatcacctga taaaatttgg acatcccttt tttggagtac accccataga ttccagtcat
     1141 ggcgtccagc aatgcctctt cccgttcctt caccagcaac gtcatcaatt ttcggttttc
     1201 acggccttgt tgcaacatcg tgataatccg atcccgattg tgaaacatcg cctgggccag
     1261 cgcgctttcc ttgtctgaaa gttccagttc gagttctgac cggagctgtg attcacgacg
     1321 gtgccggagc cccacataag taaaactgac tgagttgatg acacataaca gtgcagccac
     1381 aatccagatt ggttgttttt ggtgaactct atggctggcc tcctgggcat tgagggtact
     1441 gaggcggtca atctcggttc tgcaaatttt gaccagtggc tcgaaatcgg cttcctgggc
     1501 tttccgggtc aaccctgatg ccttatccgc tgcctggtac acttcagaat ggacattttg
     1561 catcagatca agggttttta attccttgat tctggtgttg accgcctggg atgatttttc
     1621 aatcgcagcc tgctctaaat cacctaatgc gagatagatc tttccataga ctgcgacctg
     1681 gggttggtca atgatttgaa cgattgcggt ccccaccaac accaaattca gaaatgcaaa
     1741 caaaaaatag cgacgcatgc cttgacccca atctggcaat ttttgccctt gtgtctggcc
     1801 cagccgtggg acttactggt cggatgtatc cagcgggccg gttttttgat ttcggttgcg
     1861 gagaaattct ggtgcccggt gttttcctga gaggcgacct gatttgcgat aggacccacc
     1921 aatagccgca tcttcgtctc gttcggcggc cttcaaaacc atgctgttgt aaatcaggct
     1981 ctgctcgacc gcatttttta aagcagtgcc aaatgattct tccagaatca acgtctcggt
     2041 gttttcagta ccaatcgcca gataataggc atacggatca tcgctttgag ttctgagcag
     2101 cgcctgaatt tgtttatttt tggttgggcg catcacttcc tggatcaaca ccggcaatgt
     2161 cgcctgctgg actccgtttt catcaagtga atagcgatgt ttcggcggca gaatcttttg
     2221 ggcaataatg gcctgcaaat ggttgctgat tgaaaagaaa tcatcaccgg tcatctgaag
     2281 aaatttcagg agtgctgccg gggcattggt tgaggaatgc atggtgccaa tcaccagatg
     2341 cccggttttg gccatggcca gcgcctgatt ggccatatcc tcatcgcgga tttcacctac
     2401 cacgatgatg tctggatcct gacgaagcgc gtgtttggcg ccttcggtgt agctggcaac
     2461 gtcgcgaccc acataaatct ggcggaaccg tgcccggcga ttggtaaacc gatattccgg
     2521 cggatcttca aagagcgtga tattgactgg taaccgatca ttgacggctt cgagcatggc
     2581 gcccaccgtg gttgacttgc ctgagccggt tgacccaacc accaggatca acccactgct
     2641 gatggtgcca ttcatcaaat catacaccag tgacccggcg gcgggtgaaa accggagttg
     2701 tttgagggtc ggcagcactg gccagattcg ccgaaatgac aggcataacc cggagccaag
     2761 cggagaaacc ggctctgatc ggtagacatg cacccgatag cgccctttga tctcgttgcc
     2821 ttcttcatca caggtca
//
LOCUS       DAOQDO010000635         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29112_length_2835_cov_2.874820, whole genome shotgun sequence.
ACCESSION   DAOQDO010000635 DAOQDO010000000
VERSION     DAOQDO010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29112_length_2835_cov_2.874820"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKZ53_10955"
     CDS             complement(<1..1040)
                     /locus_tag="PKZ53_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNJ40999.1"
                     /translation="MRSDPISTLEGNTLGLKANQIKRLEKLYQRRLSPRALVTQEFAR
                     QLTEISSEIRRQIGVLINRQGQVEYVIVGDAKGIVIPDLKRHRTGQGRFRGLRCIHTQ
                     LAGEGLTQDDLTDLALLRLDMMAVLSITSDGLPGWIEAAHLLPASPKLTSAQLPPPDP
                     LDDLLTESASSDEVTAGEASSAPWAILPKTPPSQLSVDFLELIEQLEAEFARTAGRRS
                     AKDRRDRAILVTVTTQSLLQAQESIEELHELAVSSGLVVVDMILQKRQQLDPRTVLGK
                     GKLQELIIRSLRQSADMIIFDQDLSPSQVRSIEGATDLKVIDRTQLILDIFAQRAKSR
                     EGKIQVELAQLK"
     gene            1521..1982
                     /locus_tag="PKZ53_10960"
     CDS             1521..1982
                     /locus_tag="PKZ53_10960"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNJ41000.1"
                     /translation="MKDEEIIYQITRSVYRKLGQYADRTLVEDMVTDIFRSIRPALGD
                     PSSSAPAPIVSGAPPEVGGSNRAVISIFGQDGAGIIAAVARVLGDAECSIVDINQTVL
                     RGKFAMMLIADISTIRGSLTDLQQKLKTEASQLGVKAYVQREDLFHAMHRV"
     gene            2166..>2835
                     /locus_tag="PKZ53_10965"
     CDS             2166..>2835
                     /locus_tag="PKZ53_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41001.1"
                     /translation="MPQHGVSPQFQLSVARSPAHFTLKLYRTPTWGISFLVIWLVIGL
                     FPLSVLQMAVQATPQLPPLSPPSDSSATPQLPFSELLASNSIRFSTFTDQDGLPQNLV
                     HAMAMDDKGHLWVGTQNGVAFYNGRDWRRVSLPDEVNSNIINQNALCATPDQSLWFGT
                     RDSGAVRLQNGNWTIFNTQQGLASNNVNCIIPQAGQPKGGSSVWVGTYGGGVSYFEQG
                     KWVTY"
BASE COUNT          676 a          752 c          715 g          692 t
ORIGIN      
        1 tacttgagct gggccagttc aacctggatc ttcccttccc ggctcttggc ccgctgggca
       61 aagatgtcaa ggattaactg ggttcggtca atgactttta agtcggtggc accttcaatt
      121 gatcgaacct gagatggaga taaatcctga tcaaagatga tcatatctgc tgattgccgg
      181 aggctgcgaa tgatgagttc ctgcagtttc ccttttccaa ggacggttcg aggatcaagt
      241 tgctggcgtt tttgaaggat catatccacc accaccagtc cgctcgacac ggccagttca
      301 tgaagctctt cgattgattc ctgggcttga agtagacttt gagtggtcac ggtgaccaga
      361 attgcccgat cccggcgatc tttggcactt cgccgcccag ccgtacgggc aaactcagct
      421 tcaagctgtt caatcaattc aagaaaatca accgataatt gactgggtgg cgttttgggg
      481 aggatggccc agggcgccga actggcttcc ccggctgtca cctcatcgga ggaggccgat
      541 tcggtcagca ggtcgtcaag tggatcaggt ggggggagct gggcagaggt tagtttcgga
      601 gatgccggaa gtaaatgagc ggcctcaatc caacctggca atccgtctga agtgatactc
      661 aggacggcca tcatatcaag tcggagtaag gccagatctg tcaggtcatc ttgagtcagt
      721 ccttcgccag caagctgggt atggatacat cgcagacctc gaaatcgccc ctgccccgtt
      781 cggtggcgtt tcaaatccgg gatgacaatc cctttggcat cgcccacaat gacatattcc
      841 acctgacctt gccgattgat caaaacacca atctggcgac ggatttcaga tgaaatttca
      901 gttaactgac gcgcaaactc ctgggtaacc agggctcgcg gtgacaggcg acgttgatac
      961 agtttttcga gacgcttgat ctgatttgct ttgagaccaa gggtgttgcc ttcaagagtt
     1021 gaaataggat cactcctcac caactgaatc aaacccacca tccaatatca acccaaacca
     1081 catcagccca gatgggttga caggaccaga ctgaagcggt ggatagttgt tcgggttagg
     1141 gctttaaaag ccggtcgaaa ttgaatacac gggatgggga gaacgacttt tggaaaaccg
     1201 gctgatttta ctctcgacca aactcgtgag caagggcagg ctgaaaaacc caggactgag
     1261 ggctaagggc ttggggctga agaagacggg atgaagacaa tgggttcaat cccctgagcc
     1321 cgatgtcttc cccccaagcc ctcagccctg gtttttgtca gcccgaagtt ttcatgacaa
     1381 aaccaggcaa gttaagttag catagcgacc gttgacttcc atttcgctcg ctcagaaacg
     1441 tttctgttcc ttccccaacc cacaaccaca gaccagcctg gccctgtggt aacaccatca
     1501 ggtttgattc tgactgcgcc atgaaagacg aagaaatcat ttatcaaatt acccgctctg
     1561 tgtatcgcaa acttggccaa tatgctgacc gcacgctggt tgaggatatg gtgaccgata
     1621 tttttcgctc gattcggccc gctcttggcg acccctcatc atcggcgcca gcaccgatag
     1681 tgtcaggcgc cccacctgaa gtgggcggaa gcaaccgcgc cgtcatttcc atttttggtc
     1741 aggatggtgc cgggattatc gctgccgtcg ccagggtttt gggcgatgcc gaatgcagta
     1801 tcgttgatat caatcaaacc gttctccgcg gaaaattcgc catgatgctg attgcagata
     1861 tcagcaccat ccgggggtca ttgactgatt tacagcaaaa attaaaaacc gaggcttctc
     1921 aactgggtgt taaagcctat gtccagcggg aagacctgtt tcatgccatg catcgggtct
     1981 aggtgggaac tgggcaactg aagggatgaa gcatgaaata aaatcacttc ttcattctgg
     2041 agttttcaac tctgagcgta tgaattttta gccctcagcc ctggcccttt cagtgaattt
     2101 tccagccatc tgtgaaaatt caccacgcag tttttggctc cctcaaatga ctcaaccaac
     2161 tgcatatgcc ccaacacggc gtctcccccc aattccagct ttctgtcgcc aggagcccag
     2221 cccatttcac cctgaaactg tatcgaaccc cgacctgggg aatcagtttc ctggtgatct
     2281 ggcttgtgat cggtcttttt ccactttcag ttttacagat ggccgtccag gcaacacccc
     2341 aattgccgcc cctatctcct cccagtgatt cctctgccac ccctcagctt ccattttctg
     2401 aactcttagc ctcaaatagc atccggtttt cgacatttac tgatcaagat gggctgccgc
     2461 aaaacctggt tcatgcgatg gcaatggatg acaaaggcca cctgtgggtt ggcacccaaa
     2521 atggagttgc gttttacaat ggacgagact ggcgccgggt ctcacttcca gacgaggtca
     2581 attccaacat catcaaccag aatgccctct gcgccacccc tgatcaaagt ttgtggtttg
     2641 gcactcgcga tagcggtgca gtccgcctgc agaatggaaa ctggacgatc ttcaacaccc
     2701 agcaagggtt agcgagcaat aatgtcaact gtattattcc ccaggcgggt cagccgaaag
     2761 gtggttcgtc ggtatgggtg ggtacctatg gcggcggggt ttcttatttt gagcagggaa
     2821 aatgggttac ctacg
//
LOCUS       DAOQDO010000636         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29144_length_2833_cov_3.598992, whole genome shotgun sequence.
ACCESSION   DAOQDO010000636 DAOQDO010000000
VERSION     DAOQDO010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29144_length_2833_cov_3.598992"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1222
                     /locus_tag="PKZ53_10970"
     CDS             74..1222
                     /locus_tag="PKZ53_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ41002.1"
                     /translation="MAIKGETVHTMAGAPIKNGVVLIRDGKIEQVGPAEQVKIPTGYR
                     TLSAKVVTPGLIDAHTVIGLSGYLNQPQDQSQLDQSEAMQPELRAIDAYNAQERLVEW
                     VRGFGVTTLHTGHGPGALISGQTMIVKTTGDTVESALVNPTSMVAVTLGSGSLAREGK
                     SPGTRAKQIALLRSELIKAQEYVTKRDTAPDDKKPARNLRMETLGQVIKGELRLLVTV
                     HRAEDILSALRVAKEFKVQIVLDGAAESYLVKDQIKAAGVPVIIHPTMFRSNGDTENL
                     SMETASALRKAGIPVALQSHFEGYVPKTRVVLFEAAVAAANGLSFEEALGTITIEAAR
                     ILGIANRVGSLEAGKDADVVLFDGDPFEYASHTTGVVINGKIVSEEAR"
     gene            1425..>2833
                     /locus_tag="PKZ53_10975"
     CDS             1425..>2833
                     /locus_tag="PKZ53_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41003.1"
                     /translation="MAHGNQDLFQSVTRGLTILFGLLIGAVWSSLTVLALDPHKAVSQ
                     YIHHAWRDELPQGSVLCITQTQDGYLWFGTYEGLVRFDGVRFVVFNKQNTPAIQSNRI
                     VSVYEDHQRRLWIGTIGGGAVCYADGRFTSYTTREGLLNNTVRAFFQDRNETLWIGTD
                     GGLTTWKDNRFSSFTTAQGLSSNLILTFLQDSTGKLWIGTQGGGINLFDGKQFRIFST
                     EAGLPDPSIRAMCELQDHSVWIATEKGLCRFANGCFSPVSSQPGLLSSFIWAVYQDRD
                     QNIWDGTNGGGVSRAVNGVFSSYTETHGLSQNFVRCFFEDREGNLWIGTNNGLNRLQN
                     GKFTPLTTTEGLSGNYVRSISQDPAGRIWIGTDGGGVNCLENGKVTRYTTADGLLSNL
                     VKSVFPGRTGQVWIGTDRGLHCLENGKLRVYTRKEGLSYEVVHSILEDRAGRLWIGTD
                     GGGLDVFENNIFTAYTTAQG"
BASE COUNT          676 a          716 c          773 g          668 t
ORIGIN      
        1 attctttttt cttttctttt tatgtgtgtt gagtgtggct gggtctcccc tgttgaaccc
       61 ggtccaggcc caggtggcca tcaaaggcga aacggttcac accatggctg gcgccccaat
      121 taaaaatggc gtggttttga tccgggatgg aaaaatcgaa caggttggac cggctgaaca
      181 ggttaaaatt ccaaccggct atcgaacact gagcgccaaa gtggtgacac cggggctcat
      241 tgatgcccac accgtgattg gactttctgg ctacctgaat caaccccagg atcaatccca
      301 gcttgaccaa tcagaggcga tgcaacctga actccgggca attgatgcct ataatgccca
      361 ggaacgactc gttgagtggg tccgtggatt tggcgtcacg accctccata ccggtcatgg
      421 ccctggagcc ctcatttcag gtcaaaccat gattgtcaaa accacgggcg acaccgtgga
      481 atcagcgctt gtcaacccaa ccagtatggt ggcggttacc ctgggaagcg gatctctggc
      541 ccgggaaggt aagtcacccg gtacgcgggc caagcaaatt gcgttgctcc gcagcgaatt
      601 gatcaaagct caagaatatg taaccaaacg cgataccgcc ccagatgaca aaaaaccagc
      661 ccgaaacctt cggatggaaa ccctcggtca ggtcatcaaa ggcgaacttc gactgctggt
      721 tacagtccac cgtgcagaag atatcctgtc agcactccgg gtggccaaag aattcaaggt
      781 gcaaatcgtt ctggatgggg cggctgaaag ctatcttgtc aaagaccaga tcaaagctgc
      841 cggagtgccg gtcatcattc accccaccat gttccgatca aacggagata ctgaaaacct
      901 cagcatggaa accgcttcgg ccctgcgaaa agctggaatt ccggtcgcgc ttcaaagtca
      961 ttttgaaggc tatgttccta agactcgggt cgtgttgttt gaagccgccg tggccgccgc
     1021 caatggcctt tcttttgaag aagcacttgg caccatcacg attgaagccg cccgaatttt
     1081 gggcattgcc aaccgggttg gatccctgga agccggcaaa gatgccgacg tggtgctctt
     1141 tgatggtgat ccatttgagt acgccagcca tacaactggc gttgtaatca atgggaagat
     1201 tgtcagcgag gaagcccgat aggaggttgg ggctggttcc tggtgcctgg ttcttggttc
     1261 ttggttcttg gttcttcatt cttcgtacct ggttcctggt tcttggaagg tattggtttc
     1321 caagcaccag gcaccaagca ccaagcacca ggcaccaagc accaagcacc aggcaccaag
     1381 catcaggcac taagcaccag gcaccaagca ccaaacactt gatcatggcg catggcaatc
     1441 aggatttatt ccaatctgtc acacgaggtc tgaccattct ttttggcctg ctgataggtg
     1501 ctgtttggag ttccctgacg gtgctggcgc tcgacccgca caaagcagtt tcgcagtaca
     1561 ttcatcacgc ctggcgcgat gaactgccac aaggttcggt cctgtgcatc acccaaactc
     1621 aggacgggta tttatggttt ggcacgtatg aagggctggt ccggtttgat ggcgtgcggt
     1681 ttgtggtttt taacaaacaa aacacacccg cgattcaaag caatcgaatt gtctccgtct
     1741 atgaagatca ccagcgccgg ctctggattg gcaccattgg cggcggtgcc gtgtgttatg
     1801 ccgacggcag gtttacgagc tacaccacga gggaaggact acttaacaac acggtccggg
     1861 ctttcttcca ggatcggaat gaaaccctct ggattggaac ggatggcggt ctcactacct
     1921 ggaaagacaa ccggttctca agctttacca cggcacaggg gttgtcgagc aacctgattc
     1981 tgacattcct gcaggactca actggaaaat tgtggattgg aactcagggc gggggcatta
     2041 acctgtttga cggaaaacaa ttccggatct tttcgactga agctggcctg ccggatcctt
     2101 cgatccgcgc catgtgtgaa cttcaagacc acagcgtgtg gattgccacc gaaaaggggc
     2161 tctgtcgctt tgccaatgga tgtttttcgc cggtttcctc acaacctggg ttgttgagtt
     2221 cttttatttg ggctgtttat caggaccggg atcagaatat ctgggatggt accaatggcg
     2281 gaggcgtgag ccgggccgtg aatggagttt tttcctcata cactgaaact cacgggttgt
     2341 cacaaaattt tgtccgatgc ttttttgaag atcgtgaagg caatctctgg attggcacca
     2401 ataacgggtt gaatcggctg caaaacggaa agtttactcc gttgacgacg accgaaggac
     2461 tgtccggaaa ctatgttcgc agcatcagcc aggatccggc aggccgaatc tggattggaa
     2521 cggatggtgg cggggtcaat tgtcttgaaa acggcaaagt gacccggtat accaccgccg
     2581 atgggctgct gagcaatctg gtgaaatcgg ttttcccagg tcgaaccggt caagtctgga
     2641 ttggaacgga tcgcggcttg cactgtctgg aaaatggaaa attacgggtt tacacccgca
     2701 aagaaggtct cagctatgaa gtcgtccact cgattttaga agaccgggct ggcagactct
     2761 ggatcgggac ggatggtggt gggttagatg tctttgagaa taatatattt acagcctaca
     2821 ccacggctca agg
//
LOCUS       DAOQDO010000637         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29162_length_2832_cov_2.533669, whole genome shotgun sequence.
ACCESSION   DAOQDO010000637 DAOQDO010000000
VERSION     DAOQDO010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29162_length_2832_cov_2.533669"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /locus_tag="PKZ53_10980"
     CDS             complement(<1..2487)
                     /locus_tag="PKZ53_10980"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41004.1"
                     /translation="MFLLSSTVTFSSARSSLQSPLPQTLNPVFQIPNPEPRTLNPGEK
                     HTHTFTLKANDYLKLVVEQLGIDVVVRLMGPDGKVVIEVDSPNGTQGPEPLAVIVEQA
                     GTYTLEIESLEKTAPPGKIELKLEPIRPATEQDKTELEVNRLNKECENLFIAGKIDQA
                     QSTIQQAVDKAEQALGPQHPALAESLNNLASLYKVRGDYPKAESLVQRALAINEKAWG
                     PNHPTVAVSLNILATFYLERGNFPKAEPLFRRALDIREHAPNPNQQEIAQTLSNLGTL
                     FASKGDFVSAEQFYQRAVPLFEQSVGPDHPELAVTIVNLAEIYQRKSDYAKAEQLYLR
                     GLAIQEKWFGPNHPNLAFTQISLATLYRNLGNTTKAEPLFQQGLATLEKTLGPNHPTL
                     AQALNNFAVVYRMKGDFSKTEAIYERSLAILEKSFGSNNPSVAFTLNNLALLYCDKGD
                     YTKAESVFQRSLAIREKVLGPDHIDVAQSLINLGNLYSQLTGDSAKTEECMHRALSIL
                     EKMPDPAYLMIGICYNTLAGINRTKGDYAQAEALYNRALSTLEKGLGPESPFVAVSLF
                     DLGALYQRTGDLAKAEPLLQRAVSIQETATGAAHPTSTRYLNGLSLFYQLKGDLTQAV
                     QYRKQANDGTERDLALNLVSGSEHQKALYLKKTQNYTDQTISLHVQAAPHNLEALKAA
                     VTVVLRRKGRSMDAMTSELAILRNQSNPEIQKLLDDYAGLAGQISVLTLRGPDKKQPK
                     EHLAEIQALENQKDALENEISRRSLEFKIQTAPVTLAGIQTLIPADTALVEYAVYRPF
                     DVKTATFGKLRYVAYVVKPGSGFRVPGSGSF"
BASE COUNT          612 a          686 c          791 g          743 t
ORIGIN      
        1 aaaagacccg gaacccggaa cccggaaccc ggaacccggt tttaccacat aggccacata
       61 ccggagcttt ccaaaggttg ctgtcttgac gtcaaaaggc cgatacacag catattcaac
      121 caatgccgtg tccgccggga tcagcgtttg gatgccggcc agtgtgaccg gggccgtttg
      181 gattttaaat tccagggatc ggcggctgat ttcgttttcc agcgcatctt tctggttttc
      241 cagcgcctgg atttcagcca gatgttcttt gggctgtttt ttgtcagggc ctctcagggt
      301 gagaaccgag atttgaccag ccaggccagc atagtcatcc agcagttttt gaatttcagg
      361 attgctctga ttccgcaaga tcgccagttc gctggtcatg gcatccatgc tgcgcccttt
      421 tcggcgcaaa accaccgtga ctgcagcttt aagtgcttcc agattgtgcg gcgccgcctg
      481 aacgtgcagc gaaatagttt ggtctgtata gttttgtgtt tttttcagat agagcgcttt
      541 ttggtgttca gagccagaaa caagattgag cgccaggtcc cgctcggtgc cgtcgttggc
      601 ttgttttcga tactgaacgg cctgtgtgag atcacctttc aattgataaa acaaggacaa
      661 tccgttgagg tatcgagtgc ttgtcgggtg ggcggcgcca gtggctgttt cctgaatcga
      721 caccgctcgc tggagaagcg gttcagcttt tgccagatcg cctgtgcgtt gatagagggc
      781 cccgaggtca aagaggctaa cggcgacaaa gggactctct gggccaagcc ctttctccaa
      841 ggtggaaaga gcccggttat agagggcttc ggcctgtgca tagtcacctt tggtccgatt
      901 gatcccggcc agcgtgttat agcaaatccc aatcatcagg taagccgggt caggcatttt
      961 ttcaaggata gagagggccc ggtgcataca ctcttcagtt ttggcgctgt cgccagtgag
     1021 ttgactatag agattgccca ggttgatcag gctttgagcg acatcaatgt ggtcaggtcc
     1081 aagtactttt tctcgaatgg ccagggatcg ttgaaagact gactcggctt tggtgtaatc
     1141 gcctttgtcg caatagagca acgccaggtt gttgagggtg aaagcaactg atggattatt
     1201 ggaaccaaac gacttttcca ggatggccag cgaccgttca tagatggctt cggttttgga
     1261 aaagtcgcct ttcatccgat acaccaccgc aaagttgttg agcgcttgag ccagggttgg
     1321 atgatttggt cccagggtct tttccagggt tgccagccct tgctgaaaga gtggttcggc
     1381 tttggtggta tttcccagat ttcgatacag agttgccagg ctgatttggg tgaatgccag
     1441 atttggatgg tttggcccaa accatttctc ctggatggca agaccacgca ggtagagttg
     1501 ttcggccttc gcatagtcgc tttttcgctg gtagatttca gccagattga caatggtcac
     1561 ggccagttcg gggtgatccg gacccacaga ttgttcaaag agcggcacgg cccgctggta
     1621 aaactgttcg gcactcacga aatcaccttt actggcgaaa agtgtcccca ggttgctgag
     1681 tgtttgggca atctcctgtt gatttggatt gggggcatgt tcacgaatat ccagcgcccg
     1741 gcgaaagagc ggctcggcct ttggaaagtt ccctcgctcc aggtaaaagg tcgccaggat
     1801 gttgaggctc accgcaactg tcgggtggtt tggtccccag gctttctcat tgatggccag
     1861 tgctcgttga accagggatt cggcttttgg atagtcgccg cggactttat acagcgaagc
     1921 caggttgttg aggctctcag ccagggctgg atgttgcgga cccaaagctt gctcggcctt
     1981 gtctacggcc tgttgaatag ttgactgcgc ctgatcaatc ttcccagcta taaacaggtt
     2041 ttcacattct ttgttgagtc ggttcacctc aagttcggtt ttgtcttgtt cggtggcggg
     2101 gcggattggt tccagtttga gttcaatttt tccaggcggc gccgtttttt ccaacgattc
     2161 aatttcaagt gtgtaagtgc cggcctgctc gacaatcact gcaagcggtt ctgggccctg
     2221 agtgccgttg gggctgtcta cctcaatcac aactttccca tccgggccca tgagccgcac
     2281 cacaacgtca attcccagtt gctcgaccac cagtttcagg tagtcattgg ctttgagcgt
     2341 gaacgtgtgc gtgtgttttt caccggggtt cagggttcgg ggttccgggt ttgggatttg
     2401 gaagaccggg ttgagggttt ggggcagggg gctttgaagg gatgacctgg ctgaggagaa
     2461 agtaacggta ctgctcagaa ggaacattgc tcctgacact acccataaca aaagcctgca
     2521 agatgatttt tgcggcatac aacccctctc agctttcaag ccaatcagtt gaagcaattt
     2581 ggaaaaagga aatgagttga ggaaacccaa aaaaattgta actacagaga gtggtacatt
     2641 atcaaaaatc agcgagacag tgacaagaaa acaaaaatga caaagcgacc attgatcact
     2701 ttgtcatctt gttacccgtg aactgctgcc gcaacgagtc tgtgctgtaa cctatccccg
     2761 ctgttcgtcc gccgaccccg accgcgatgt cttcgccgaa ggggaagaag tcccttcgat
     2821 tgacgacgat gc
//
LOCUS       DAOQDO010000638         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29210_length_2829_cov_3.210526, whole genome shotgun sequence.
ACCESSION   DAOQDO010000638 DAOQDO010000000
VERSION     DAOQDO010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29210_length_2829_cov_3.210526"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2503)
                     /locus_tag="PKZ53_10985"
     CDS             complement(158..2503)
                     /locus_tag="PKZ53_10985"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNJ41005.1"
                     /translation="MSKPVASLSQPVQSIGINAQTVIARRYARKDINGQPLETWPEIV
                     SRVVKYVAKAESDPDHRHQFHQEATQLLLNRQFIPNTPCLVNAGHERAQLAACFVLPI
                     ADSIASIMEHAKQCAIIHQTGGGTGMTYELLRPAGAIVKSTHGVASGPVSFMNIVNAT
                     TETIRQGGVRRGANMGILAIQHPDILRFIHAKNDQTSLTNFNISVTVTDAFLQAVERN
                     EWFQTEFDGKPWVQPIIDPLTGQNYVFEGTEPPQPGMVFAPDVWQRIVFSAWKYAEPG
                     IIFIDEVNRRNHVLKSLGRISASNPCAEQMLHAYNACNLGSLDVAKFANEAGEIDWAG
                     LQKAIRTCVRFLDNVIEMCFWPLPEVKQMVQRTRPIGLGVMGFADLLLKQKIRYASPE
                     SLKCAEALMSFVRREAWRASCELGKEKGVFPEFESNRDAYESFFENELRVPATECTPR
                     NYEVTTVAPTGTISLVAETSSGIEPNFSWAYVRHDTIGTRVYVHPLAARALGIEFDPQ
                     DPTSMERAATVVCERQRQLPAEFITSHEISPDDHLQILAAFQKHVDNSISKTVNGPAS
                     HTVEQIDQLFRTAARLGVKAVSYYRDSSREGQVLTQLASSKPSEAPAPVSTPPEETAT
                     VIEPTTKNRPKELQGWTWQIPFEGQNLYVTVNHDGTNVLEVFATGPVSGSVGMLVSRM
                     LRGGFTPPEVARILNKVTGTHATWFNRRCLSSPEQAIAECILLTMNRLEGKIEPPSGK
                     VASVKATTLRTGYRACPECGSEVNHASNCDICPVCGWSKCT"
BASE COUNT          665 a          696 c          752 g          716 t
ORIGIN      
        1 atcgggctga agaattggtt ttatttcatc cctcatcctt catccctcat cccttcaatt
       61 gccccgagct tgcgagtctt cagccccaag cccaggtttt ttcagcccca agcccaggtt
      121 ttttcagccc ccagccctgg gtttttcagc cctggctcta agtgcatttc gaccagccac
      181 aaaccgggca gatatcacag tttgaggcat ggttgacctc gctgccgcat tccggacagg
      241 cgcgataccc ggtacgcaaa gtggttgctt tgacagacgc gacttttccg gatggaggct
      301 cgatttttcc ttccaatcgg ttcattgtca ggaggataca ttcagcaatc gcctgttccg
      361 ggcttgaaag acagcgtcgg ttaaaccacg tagcgtgggt tcccgtgacc ttatttaaaa
      421 tgcgggcgac ttcaggtggc gtaaacccac cgcgaagcat ccgcgaaaca agcattccaa
      481 ctgaaccgct caccgggcca gtggcaaaaa cttcaagcac gttggtgccg tcgtggttga
      541 ctgtgacata gaggttttgg ccttcaaatg gaatttgcca cgtccagccc tgtaactctt
      601 tcggtcggtt tttggtggtt ggttcaatga ccgtcgcggt ttcttcgggt ggagttgaaa
      661 ctggtgctgg tgcttcgctg ggttttgaac tggccagttg ggttaaaacc tgtccttcgc
      721 ggctactgtc tcgatagtag gaaaccgctt tgactcccag acgggcagcc gtccgaaaaa
      781 gctggtcaat ttgttcgacc gtgtgggatg ccggcccgtt gacggttttc gaaatactgt
      841 tgtccacatg tttttgaaag gccgccagga tctgcaagtg atcatccgga gaaatctcgt
      901 gtgaagttat aaattcggct ggaagctggc gttgtcgttc acaaacaacc gtggccgccc
      961 gttccatcga ggttggatcc tgcggatcaa attcaatccc cagtgcacgg gctgccagcg
     1021 gatggacata aacccgtgtt ccaatcgtat cgtgccgcac ataggcccaa ctgaaatttg
     1081 gttcaatgcc tgaactggtt tcggcgacaa gcgaaatcgt accggttggg gcaacggtgg
     1141 tgacttcata gttacgtggc gtgcattcgg tagctggaac tcgcagttca ttctcaaaaa
     1201 atgactcgta ggcgtcgcgg tttgattcaa actcagggaa gacgcctttt tctttgccca
     1261 gttcgcaact cgcccgccag gcttcgcggc gaacaaagct cattaaggct tcagcgcatt
     1321 tgaggctttc gggactggca taccgaatct tttgcttgag cagcaaatcg gcaaatccca
     1381 tcactccaag cccaatcgga cgggtgcgtt gaaccatctg tttgacttcg ggaagcggcc
     1441 agaagcacat ctcaatgaca ttatcgagaa accggacgca ggtgcggatt gctttttgaa
     1501 gcccggccca gtcaatctct ccagcttcat ttgcaaattt cgccacatca agtgagccaa
     1561 gattacaggc attataagca tggagcattt gttcagcaca gggattgctc gccgaaatcc
     1621 gtcccaaact tttgaggaca tggtttcggc gattgacctc atcaatgaaa ataattccag
     1681 gctcagcata tttccaggct gaaaagacaa tgcgctgcca gacgtcgggg gcaaacacca
     1741 tgccgggttg tggtggttca gttccttcaa acacgtaatt ttgcccggtg agcgggtcaa
     1801 tgatgggttg cacccagggt ttgccgtcaa attcggtttg aaaccattcg ttgcgttcaa
     1861 ctgcctgtaa aaacgcatcg gtcaccgtca cggaaatatt aaagttggta agactggtct
     1921 ggtcattttt ggcgtggata aaccgcagaa tatctggatg ttgaatggcc agaattccca
     1981 tattcgcgcc acgtcgaacg cctccctgtc ggatcgtttc ggtggtggca ttgacaatat
     2041 tcataaacga aaccggaccg gaggccacgc catgggttga tttgacgatg gcaccagcgg
     2101 ggcgaagcaa ctcataggtc attccggttc cgccgcccgt ttgatgaatg atggcgcatt
     2161 gtttggcgtg ctccatgatg ctggctatcg aatcggcaat ggggagcaca aaacaggccg
     2221 cgagttgagc gcgttcgtgg ccagcattta ccagacaggg agtgttcgga ataaactggc
     2281 gattgagcaa caactgggtc gcttcctggt gaaactggtg acggtggtcg gggtctgatt
     2341 cagctttggc gacatatttg accacacggg agacaatctc cggccaggtt tcaagcggtt
     2401 gtccattaat atctttgcgg gcatagcgtc tggcaatgac cgtttgagca ttgatcccaa
     2461 ttgattgaac tggttgagag agcgaggcaa ctggcttgga cataatggtt ttcatctgta
     2521 aacaaggaca ggttgagtag atttccgcag cacgctaacc agttaaaaga aagcaatgat
     2581 ttgataagtc atttaaaata aacttattag cgtgttgatg agtaaaatgg aaaaagaacg
     2641 aatgtacaca gagcaatttc ctggccgaag tcgaagccag aaaaaaggtg tttacagggt
     2701 agttggatcc ggggtcttct ggatgtgact taaatcatcg agggttgagg gagtgaaaag
     2761 ccgggttccg ggttccggtt cccgctcatc agcgccacca gttatcttca gtcccaaaat
     2821 ccttccacc
//
LOCUS       DAOQDO010000639         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29343_length_2822_cov_2.744489, whole genome shotgun sequence.
ACCESSION   DAOQDO010000639 DAOQDO010000000
VERSION     DAOQDO010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29343_length_2822_cov_2.744489"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKZ53_10990"
     CDS             <1..189
                     /locus_tag="PKZ53_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004882565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41006.1"
                     /translation="RQQEGSDHHTPITALTAGALQTDADKCYQAGMDDFLAKPFKPEE
                     LRIILQRWLNQAETNLEL"
     gene            complement(372..2138)
                     /locus_tag="PKZ53_10995"
     CDS             complement(372..2138)
                     /locus_tag="PKZ53_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ41007.1"
                     /translation="MNFSSLTRKKSISLIQKDAAAGFSDGEGHGGGGLVRTLTTLDLT
                     ALGIAAIIGAGIFSTIGNAAFNGGPAVVFLFIFTAIACGFSALCYAQFASMIPVSGSA
                     YTYAYAAFGELAAWIIGWDLLMEYAIGNIAVAISWSDYFTALMASFGIQIPQYLTMDF
                     LTASRGAEAVTQLLAKGQTLTQIQTESKMLYEAYLAWTTAPHLVGPLRLVCDLPALVI
                     TLLITTLVYVGIRESKKASNAMVALKVTIVLFVIVLGAFYVKTENWHPFAPNGIPGVL
                     KGISGVFFAYIGFDAISTTAEECRDPQRDLPRGMIYSLIICTILYVLISLVLTGMTSY
                     KTLQVGDPLAFVFGPQVANIHWVEKIVAVSAVIAMATVLLVFQLGQPRIWMSMSRDGL
                     LPKIFSAIHPRFHTPWFSTIITGFLVAIPALFLNLTEVTDLTSIGTLFAFILVCGGIL
                     MLDSSKIEAKFKVPYVNAKFIIPVLFILFWVVGFVKYPTELTRFFTGVDLADPGLSGW
                     EVIQHRIPTYAFILISAGLSVMCFLKNLSLIPVLGLLSCGYLMSELGITNWVRFLVWL
                     AIGLMLYFTYGYRNSKLARASA"
     gene            2365..>2822
                     /locus_tag="PKZ53_11000"
     CDS             2365..>2822
                     /locus_tag="PKZ53_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNJ41008.1"
                     /translation="MSHLHPLKFSIPFVTGNIMLISYQGKLPVFDETVWIAESAQVIG
                     DVRVGSESSVWFGCVIRGDVEPIRIGHHTNIQDASVLHVTGGRFPLTIGNYVTMGHRV
                     LAHGCTIGDRCLIGMGAIILDGAVIGDESVVAAGAVVPEGMIVPPRTLVAG"
BASE COUNT          787 a          721 c          698 g          616 t
ORIGIN      
        1 cgccagcagg aaggctctga ccaccataca ccgatcactg ccctgaccgc tggcgccttg
       61 caaaccgatg ctgataaatg ttaccaggcc ggaatggatg actttctggc taaaccattt
      121 aaacctgaag agttgcggat tatccttcaa cgctggctga atcaagctga aacgaatctg
      181 gagttatagg tttccagaat cagaagccac aattccctac agctggttct agccagttca
      241 atttcatttt ggcagggaac gtaccggcca acgcaaccga gcctgagttt gccctcaagg
      301 agatgccgga acgttgtttc tcgcatcccc tctggcacca gccacccgag atcttttttc
      361 ttcctttttc ttcaggcact tgccctggca agttttgaat tgcggtagcc atacgtgaaa
      421 tagagcatca atccaatagc cagccagacc aggaatcgca cccagttggt aattccgagt
      481 tcggacatca aatatccgca acttaacagc cccaaaactg gaatcaacga tagatttttt
      541 aagaaacaca tcaccgacaa tccggctgaa attagaataa aggcataggt cggaattcga
      601 tgttgaatca cttcccaccc tgacaggcct gggtcagcca gatcaacacc ggtgaaaaaa
      661 cgggtcaact cggttggata tttcacaaaa ccaaccaccc aaaacaagat aaagagcacc
      721 ggaatgatga atttagcatt cacatacggc accttgaatt tggcttcgat cttgctgcta
      781 tccaacatca aaatgccacc gcataccaga atgaaggcaa acaatgtccc aatgctggtc
      841 aggtcggtca cttcggtcaa attcaggaag agcgccggaa tggccaccag gaatccagtg
      901 atgatcgtcg aaaaccacgg ggtatgaaac cggggatgga tggcggaaaa aatcttaggg
      961 agcaatccat cacggctcat tgacatccag atacgtggct gaccaagttg aaacaccagc
     1021 aacacggtgg ccatcgcaat cacggcactg acggcaacaa ttttttcgac ccagtgaata
     1081 ttggcaactt gtggaccaaa cacaaacgcc agcggatcac ccacctggag ggttttgtaa
     1141 ctggtcattc cagttaacac cagtgaaatc aacacataca aaattgtgca gataatgagc
     1201 gaatagatca ttccgcgtgg caggtcgcgc tgtgggtcgc ggcattcttc agccgtggtg
     1261 gaaatggcat caaatccgat ataggcaaag aacacacccg aaatgccttt aagcacgcca
     1321 gggatcccat ttggagcaaa cgggtgccag ttttcggttt tgacataaaa cgcacccaac
     1381 acaatcacaa acagcacaat ggttactttc aaagcaacca tggcatttga agcctttttt
     1441 gattcacgaa tgcccacata caccagcgtc gtaatcaata aggtaatgac cagcgccgga
     1501 aggtcacaca ccaggcgtaa cgggccaacc aaatgcggcg ccgtggtcca ggcaagatag
     1561 gcttcataga gcattttgct ctcagtttga atctgggtca gggtttgccc tttcgccagc
     1621 aattgcgtga cagcctcagc cccacgtgaa gcagttaaaa aatccatggt caggtattgc
     1681 ggaatttgaa tcccgaaact tgccatcagg gccgtgaagt aatcgctcca cgaaatggcc
     1741 acggcaatat ttccgatggc atattccatc agcaggtccc agccgataat ccaggcggca
     1801 agttcaccaa aggctgcata cgcataggta taagcagaac cagataccgg aatcatcgaa
     1861 gcaaactggg cataacacaa tgccgaaaat ccgcaggcaa tcgcggtaaa aataaacaaa
     1921 aatacaacgg ctgggccgcc gttgaaggct gcattgccta tcgtggaaaa aatcccggcg
     1981 ccgataatcg cggcaattcc aagcgccgtg agatcaagcg tggtcagtgt ccgaaccagt
     2041 ccgcctccgc catgaccttc cccatcagaa aatccggcgg cggcatcttt ttgaatgagc
     2101 gaaatagact ttttgcgtgt cagtgatgaa aagttcatgg tctaatgtct ggactcgtgg
     2161 taaaggcgcc agagcgcgaa ataaaaagac ctgaggcggc agcttcagga gaaaccagat
     2221 caagtgatca attgtgaacc gtgattgatg aaaaagtgag gcggaggcaa tgctacttgt
     2281 cacctcaccg aacgtcaact aagtgtttat cctgaccagc gttgagaagt ccttttcttg
     2341 ctctactgtg ggatcaatgg caagatgtcg caccttcacc cgctgaagtt ttcaattcca
     2401 tttgtgacag gcaacatcat gttaatcagc tatcaaggga aactcccggt ttttgacgaa
     2461 accgtgtgga ttgcagaaag tgcacaggtg atcggtgatg tcagggttgg ttcagaatca
     2521 agcgtctggt ttggctgcgt gattcgcggc gacgtggaac caatccgcat cggccatcat
     2581 accaacattc aggatgcttc ggtgctccac gtgaccggcg ggcgatttcc gctcaccatt
     2641 ggaaactatg tcacgatggg gcatcgcgtg ctggcacacg gttgcacgat tggcgaccgg
     2701 tgcctgattg gcatgggcgc gatcattctg gatggtgcgg tgattgggga cgaatccgtc
     2761 gtggcggctg gcgcggttgt gcccgaaggc atgattgtcc caccacggac actggtggca
     2821 gg
//
LOCUS       DAOQDO010000640         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29469_length_2815_cov_3.542391, whole genome shotgun sequence.
ACCESSION   DAOQDO010000640 DAOQDO010000000
VERSION     DAOQDO010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29469_length_2815_cov_3.542391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1736
                     /locus_tag="PKZ53_11005"
     CDS             216..1736
                     /locus_tag="PKZ53_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41009.1"
                     /translation="MDENPQSSQKTTDEGQEQRSQIIRAELNIERWPSIWLPSKAMSR
                     ALDSQIIRVLEKADVLDNGDTLSAKVRISADQEYGPLNTEDQKVFYALIYLWENQGKP
                     KEFTFSIVQLATLLKKDWGEFTREAIKKSLKKLRLTNFSWENSYYDKSQRKTLKNLEF
                     LTLLSNLKISEVQTDGRTNKQSCKASFFPEIEKNLKENYSRPTRFKLIIGFRSGISQL
                     IYKYLELKLYKNILHERKSKDLFDELGLISNDYKKLSHRKRTLDSALKELQNIPIQDG
                     LLSVQIETTVDKKDVKIVASKQLIEIEDSTLFKDQDIVDLNLNDQSLQDTLSSEMISP
                     STITPSALVGYFHEIFNVKRKKVNKKELEHAHKLIETYSLSKEKGQFIVEFAWEEARK
                     TNFSIQQFGGILQYVDVALTEYEKQFQNEIQGKMIAEAVLEVPEEEKEPYSSEAAIEK
                     LLSLNEDGQNDWCKRISAVIDSEKLKVILRYKPEVARETLLHIASRRFTKEILEID"
     gene            complement(1790..2059)
                     /locus_tag="PKZ53_11010"
     CDS             complement(1790..2059)
                     /locus_tag="PKZ53_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNJ41010.1"
                     /translation="MTRCWRNGAPQDRSGSPSTYPGSHGSLHAKCAIVDGRRIFISSA
                     NLTEFALNLNMEMGVLIESSDLAQQAEKHIKHLITYGLLRCLESS"
     gene            complement(2363..>2815)
                     /locus_tag="PKZ53_11015"
     CDS             complement(2363..>2815)
                     /locus_tag="PKZ53_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNJ41011.1"
                     /translation="FPGLRYLLIHSFAHALMRELALECGYTAASLRERIYSRYPQEDD
                     GPQAGVLIYTAAPDSEGTLGGLVALGEPEILGRHIERAIEALHLCASDPLCAEHDPAQ
                     VTATLHWAACHSCLFSPETSCERGNRYLDRAVISSTVKFPALAYFRSR"
BASE COUNT          873 a          635 c          616 g          691 t
ORIGIN      
        1 tgttattact ttatttcaaa aacaattcta atcaaaactc gccttaaaac accggcttga
       61 gaaactggga atcaacttct caacccagga aacctgccgt ttacaaaccc aggtggtcaa
      121 agtgaaattc aatgaatcat ttcgaggagg aaggcgcttt ctttcaaacc aaccactgaa
      181 attaccggtt tagggaatcc gcactgattt taagaatgga cgagaatccc caatcttccc
      241 aaaaaacaac tgatgagggt caagaacaac gatcccagat tatcagagct gaacttaaca
      301 ttgagagatg gccatctatc tggctaccaa gtaaagcaat gagccgggca cttgacagcc
      361 agatcattcg tgtattggaa aaagctgatg tactggacaa tggagataca ctttcagcaa
      421 aagtgagaat cagtgcagat caggagtacg gacccttaaa cactgaagac caaaaagtgt
      481 tttatgcgtt gatttacctc tgggaaaatc agggaaaacc caaagaattc actttttcga
      541 tagtccaatt agcaacttta ctcaaaaagg actgggggga atttacacgg gaagctatca
      601 agaaatcgct caaaaaactc agattgacca acttcagttg ggaaaattcc tattacgata
      661 agtcccaaag aaaaacgttg aagaacctgg agttccttac acttttatca aaccttaaaa
      721 tcagtgaagt tcaaacagat ggccgaacaa ataagcaatc ctgtaaagct tcattttttc
      781 ctgagattga gaaaaacctt aaagaaaatt atagtcgccc cacccggttc aaattgatta
      841 ttgggtttcg gagtgggatt tctcagttaa tctataagta cctggaacta aaactatata
      901 aaaacattct gcatgaacgt aaaagcaaag acctctttga cgaactgggt ctgatatcga
      961 atgactacaa aaaactttct catcgcaaaa gaacacttga ttccgcttta aaagaacttc
     1021 aaaacatccc aatccaggat gggctgttgt ctgtccaaat tgaaacaaca gttgataaga
     1081 aagatgtcaa aattgtcgct tccaagcaat tgatcgaaat cgaagattct acacttttca
     1141 aagatcagga cattgtagac ttgaacctga acgaccaatc actacaagat accttatctt
     1201 cggaaatgat ctctccctca accataaccc catcagcgtt ggttggatat ttccatgaga
     1261 tttttaatgt caaaaggaaa aaggtaaata aaaaagagtt agagcacgcc cataagctta
     1321 ttgaaaccta ttctctttcc aaagagaaag ggcaattcat agttgagttt gcctgggaag
     1381 aagcaagaaa aactaatttc tcaattcagc agtttggtgg aattctgcaa tatgttgatg
     1441 ttgctctcac cgaatatgaa aaacaattcc agaatgaaat ccagggtaaa atgattgctg
     1501 aagccgtatt ggaagtccct gaggaagaga aagaaccgta ctccagtgag gcagccattg
     1561 agaaactact tagtttaaat gaggatggcc aaaacgattg gtgtaaaaga attagtgccg
     1621 ttattgattc cgaaaaactg aaagtgattt tacggtataa gccagaggtt gcgcgagaaa
     1681 ctctacttca cattgcttcg agacgtttta caaaggaaat cctggaaatt gattaggttc
     1741 ggttgatatt tgctcactgg atggtttgaa ttccttttct tggttctctt cagcttgatt
     1801 ctaaacatcg aagaagccca tacgtgatga ggtgtttgat gtgcttctca gcctgctggg
     1861 ccaggtcgga gctttcaatc aagacgccca tttccatatt gagattcaga gcaaactcgg
     1921 tcagattggc gctggaaata aagatccggc gtccgtccac aatcgcgcat ttggcatgaa
     1981 gtgatccgtg gcttcctgga taagttgaag gagaacctga cctgtcctgc ggagcgccgt
     2041 tcctccagca tctggtcaag tgcagacccg ttcaactgaa agctcctggc gaaaagcaac
     2101 ctggcaatag gcgaaagagt gagagttaac catcacccta cctggttctc ctgctgtttc
     2161 aacctgccag aacttcatgc aagttttcga tgacctgacc aggcaaggca gtcatcgttt
     2221 ctcatcgcaa ttacacagac atccagatca cgattagaaa ccctgtcatt gcgcctaaaa
     2281 tcggttgcca gggcttttaa ccggagcgga tgaagtttta ctcatcctgg acgaatttga
     2341 gccaacaccg gggatgacag cattagcggc tcctgaaata ggccagcgcc gggaatttga
     2401 ccgtggagga gatcacggcc cggtccaaat acctgtttcc acgctcacaa gacgtctcag
     2461 ggctaaacaa acaggaatga caggcggccc aatgcaaggt cgccgtgacc tgggccgggt
     2521 cgtgttcggc acataatgga tccgaggcgc acaaatgcag ggcttcaatc gcccgttcga
     2581 tatgccgacc caggatttcc ggctccccaa gcgccactaa tcctccgagg gttccctcgc
     2641 tgtcaggtgc ggcggtgtag attaacaccc ccgcctgggg tccatcgtcc tcctgggggt
     2701 atctggaata tatccgttca cgcagcgaag ccgccgtgta tccacactcc agtgccagtt
     2761 cccgcattaa ggcgtgggca aaagaatgga tcaacagata tcgcagcccc ggaaa
//
LOCUS       DAOQDO010000641         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29472_length_2815_cov_3.255435, whole genome shotgun sequence.
ACCESSION   DAOQDO010000641 DAOQDO010000000
VERSION     DAOQDO010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29472_length_2815_cov_3.255435"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..688)
                     /locus_tag="PKZ53_11020"
     CDS             complement(359..688)
                     /locus_tag="PKZ53_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010317240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNJ41012.1"
                     /translation="MDFNEYQKQALKTAIYPNLGANLQYPTLGLCGETGEVAELVKKS
                     IRDEGGEVSPERREKLIKELGDVLWYCAMLAHELRVPLSTVAEHNIQKLADRKARNTI
                     HGDGDER"
     gene            complement(738..2693)
                     /locus_tag="PKZ53_11025"
     CDS             complement(738..2693)
                     /locus_tag="PKZ53_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ41013.1"
                     /translation="MSFLFLSIGVLLSATLLCGMAAILARGESSTSPSSRILPWLLAL
                     GTAWYLTMAGQYAIYPLLDELVKDHNRVIPAGLLRPALLLFLAGLAHCYVSELPPLIQ
                     RKIPVWLIYLPTLTMLVILAGFRLTSQYFFPVVGPFRVESKPILLMALGIVLFDLGIV
                     MFCHWQLTKSARRSLIWLGIGGLEALLLYFSFHQWPRVLAESSSYYDLLSMLLPIPLT
                     LWLAYNRARYQFFDVFIKNGALVLAIALAMGLSISGGIFYFQWFFPNAFFQQHDVTVA
                     ALVQFCLGCTFLSVPLVTVVIRWRPRLDAWVDSVLLRRPGYSQALKELLPLLQQATDE
                     ASAIDRVCVHLTQVMQAHRVEFVIGTIPETDSLTDLTQNAGGLPEIKSEVLTGDQYFG
                     FVHVVGSSQGLRYLSEDVIYCDRVASLLAGMLLTLRTQAERTRELQQAQQLRELANQA
                     HIRALQAQINPHFLFNCLNLLNSLVRTDPERARALMQRLAAIFRYTLDSTRKETVTLA
                     EELKFVEDYLEIAQARFGKRLSTSIEIEAAVQGTQVLPMLVQPLVENALHHGIEPKVG
                     PARLDIRAFAENGWVVIEVTDDGIGFATTDLSHKGQTHSKNPDTHAGIALANINDRMK
                     SQFGTTLVIESQPQAGTKVELRFPGQV"
BASE COUNT          737 a          730 c          700 g          648 t
ORIGIN      
        1 gcatcacgtg gggctgaggg cttggggctt ataccaggtt ggagccaggg ctcaggatca
       61 gcaaccagaa aagacaactc tttcaacaat ttaacctttt tatcattcga ggattttatt
      121 cctcaatggt acggaccttg tcaaaagttt cctgagcgaa tgagttttgg aagtggttgg
      181 tttttttcgt gtattttgtg tatttcgtgg ttcaacatgt ctggaaattt tcggtaaggt
      241 acttatcagg tatcgaaacc agtagagttg aagcctgatc cagaccaatc gcgtttagga
      301 tgagctaaaa tggtgcgggc caccctgtca ccctgtcaca ggaatcatcc gtcagggttt
      361 atcgctcgtc cccgtcaccg tgaatggtat tgcgtgcctt tcggtcagcc agcttctgga
      421 tgttgtgctc agccactgtt gaaagtggaa ctcgaagctc atgggccagc atggcacaat
      481 accagaggac gtcacccagt tctttgatca atttttcacg gcgctcgggt gagacctcgc
      541 cgccttcgtc acgaatgctt tttttgacca gttcggccac ttcgccggtt tcgccgcaca
      601 acccaagtgt cggatactga agattggcgc ctaaatttgg ataaatcgct gttttgagtg
      661 cctgcttttg atattcatta aaatccatga tggttcccag tctgaaggtt aaattgaata
      721 aaaaacctcg ccagttttca cacttgtcca gggaaacgca actcgacttt ggtgccggcc
      781 tgcggctgac tctcgatgac cagggtggtg ccaaactgac ttttcattcg gtcattgata
      841 ttggccagag caataccagc gtgggtatca ggattttttg aatgggtctg acccttgtgt
      901 gacaggtccg ttgtggcaaa accaattccg tcgtcagtta cctcaatgac gacccaccca
      961 ttttcagcaa acgcccggat gtcgagtcga gctggaccaa ctttgggctc aatgccgtgg
     1021 tggagcgcgt tttcaaccag cggttgaacc agcatcggta acacctgagt tccctgcaca
     1081 gcagcctcga tttcgatact ggtcgaaagt cgttttccaa atcgagcctg tgcaatttca
     1141 agataatctt cgacaaattt cagctcttca gccagggtga cggtttcttt gcgggtactg
     1201 tcaagggtgt accgaaaaat tgctgccagt cgctgcatca gtgcccgtgc ccgttccggg
     1261 tcagtacgga ccagcgaatt cagaagattg aggcagttaa ataaaaaatg gggattgatt
     1321 tgggcctgta aggctcgaat atgggcctga ttggccagtt cgcgcagttg ttgcgcctgt
     1381 tgcagttcgc gggttcgctc ggcctgtgtg cgcagcgtca acaacatccc cgcgagcaga
     1441 cttgccacac ggtcacaata aatgacgtct tcgcttaaat atcgcagccc ctgggaactc
     1501 ccgaccacgt gtacaaaccc gaaatactga tctccagtca atacctctga tttgatttcg
     1561 ggcagaccgc cagcattctg agtcagatcc gtgagtgagt cggtttcagg tatggtccct
     1621 atgacaaatt caactcgatg ggcctgcatc acctgggtca aatgaacgca aacccggtca
     1681 atggctgatg cttcgtcagt cgcctgctgg agcaagggaa gcagttcctt caatgcctgg
     1741 ctataccctg gccgtcgtaa caacactgaa tccacccagg catcaagccg tggccgccat
     1801 cggatcacca ccgtgaccag tggcaccgac agaaacgtac atcccaaaca aaactgcacc
     1861 agcgcggcaa ccgttacatc atgctgctga aaaaaagcat ttggaaaaaa ccactgaaaa
     1921 tagaaaatcc cgccgctgat tgatagtccc atggccaggg caatcgccag caccagggcg
     1981 ccatttttaa taaagacatc aaaaaattgg taccgtgccc gattgtaggc cagccagagc
     2041 gtgagcggga tcggcagcag catactcaac aaatcataat aggaactgct ttcagccagc
     2101 actcggggcc attgatgaaa cgaaaagtac aggagcagcg cttctaatcc accgatgcca
     2161 agccagatca gtgaccgccg ggcgcttttg gtcaactgcc agtggcaaaa cattacaatt
     2221 cccagatcaa acaacacaat gccgagcgcc atcagcaaaa ttggttttga ctcaactcga
     2281 aacggcccga caaccggaaa aaaatactgg ctcgtcagcc gaaacccggc caggatcacg
     2341 agcatagtca gggttggaag gtagataagc cagaccggga tctttcgctg aatcagcggc
     2401 ggcaattcgg aaacgtaaca atgtgccagc ccggccagaa ataataacaa cgccgggcgg
     2461 agcaacccgg cgggaatcac gcggttatgg tctttcacca gctcatccag cagcggataa
     2521 attgcatatt ggccagccat ggtcagatac caggccgtac ctaatgccag taaccacggg
     2581 aggattcggc ttgaaggcga agttgacgat tctccacggg ctaaaatcgc cgccataccg
     2641 cacaacaatg tggctgaaag caacacgcca atgctcaaga ataaaaatga catctcagac
     2701 aacatcagcc accctgcact tgcaaaacgg aaattgccaa ccggggttga aacaccggct
     2761 ggcaaggaaa aattatgctg gagaaatctt caacaggatg cagtataccc aggtt
//
LOCUS       DAOQDO010000642         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29481_length_2815_cov_2.249638, whole genome shotgun sequence.
ACCESSION   DAOQDO010000642 DAOQDO010000000
VERSION     DAOQDO010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29481_length_2815_cov_2.249638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKZ53_11030"
     CDS             complement(<1..657)
                     /locus_tag="PKZ53_11030"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ41014.1"
                     /translation="MKIMKWLTGIALTLLAFFPTVHAGTGRPAKPDLIGIPAALIIGN
                     ANYPTNRLRNAVNDANSMSEVLKEAGFTVTTLKDATSDQMKHEIERFTASLRGSQIGL
                     IYFSGFGFLQDEDVSLLPVEVDKSKAQTNPKAVKLSWVLDCFQRSKRKQNIVILDTCL
                     NDPFQKNNRNQIPPFRIKIPEGTLLALGTHFGGIASDGENAHGLYTQELLEVMRTPNL
                     P"
     gene            complement(756..2150)
                     /locus_tag="PKZ53_11035"
     CDS             complement(756..2150)
                     /locus_tag="PKZ53_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ41015.1"
                     /translation="MLLWPAMVCGSVPGKTQVSFIEVKKQGILNYHSKKYDEAVLYFK
                     KAVSLQPDTECYHYLALIYDILKEPQQAQRYRQLAGESTASGNQTTPAGSGPARVSTP
                     QIEILSPLAGSTVCSQYLTLRYRTSAPPETGLEVWVNNQMVERGQRVKTPAASTSIAG
                     ADGGGELQIVIPPHDTQVMLRATLPDGSKVQTSVSLRWCQEPLVTTAPENNEYKPKLY
                     ILGIGVSAYADSSLILNYPAKDATDFVGVLKQQRGLLYEDVVVRLLTDSAATHNNILD
                     GFQWIKTQTTTKDVAMIFLAGHGVDEDDTYYYLPQDADTNNLLKTGISFSSIKTTINS
                     MAGKKFLFVDTCHSGNVFGSSNRRGAPNPDINLVVQELRAAENGTVIFTASTGRQASL
                     ENSRWGNGAFTKALVEGLSGQADLRNKGKVTVSGLENYISERVKELTNGKQTPTTAKP
                     VTVIDFPLSVRINK"
     gene            complement(2298..>2815)
                     /locus_tag="PKZ53_11040"
     CDS             complement(2298..>2815)
                     /locus_tag="PKZ53_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41016.1"
                     /translation="DFQAAEPKLAQALTYYTDARTKNPAEPYFQEGASASGFLMERIS
                     RSQKECTGWREYKVTKAAMDEAEAAALAATAAKVEPPLEKQLSGDGVLRNQDVLVMVK
                     RKLSADFIRNAIVQAERTQFDLSLQEQFKLIDGGVSEAIVEVMKSNNNKPKPAPPPPA
                     PPKPINRRRRG"
BASE COUNT          643 a          709 c          721 g          742 t
ORIGIN      
        1 cgggagattc ggggttcgca tcacctccaa cagttcctgg gtatagagtc catgagcatt
       61 ttcaccgtca ctggcgattc caccgaaatg ggttcccagg gcaagcagcg ttccttcggg
      121 aattttgatc cgaaaaggtg ggatttgatt ccggttgttt ttttgaaacg ggtcattcag
      181 acaggtatcc agaatcacga tgttttgctt gcgctttgac cgttggaaac aatccaaaac
      241 ccaactcagc ttcaccgctt tcggatttgt ttgagctttg gacttgtcaa cctcaaccgg
      301 caataatgaa acatcctcgt cctgaagaaa cccaaagccc gaaaaataga ttaaaccaat
      361 ctgggagccc cttaaagaag cagtgaaccg ttcaatttca tgcttcattt gatctgaggt
      421 tgcgtctttg agcgtagtca cggtaaaccc ggcctccttg agaacctcgg acattgaatt
      481 ggcgtcattg actgcattcc ggagccggtt ggtgggataa ttggcgtttc caataatcaa
      541 ggctgctggt atgccgatta aatcgggctt cgccgggcgg ccggttccag catgcactgt
      601 cgggaaaaac gccaggagtg tcaaagcaat tcctgtcagc catttcatga ttttcatgga
      661 tgttctcctg atttggttgg tggcagccag tcagtggcag ccctgctgtt aggttcgccc
      721 gcactgtcct gtggttggtc ctgggtgggc aggcatcatt tattgattcg cacggaaagc
      781 ggaaaatcaa tgacggttac aggcttggca gtggttgggg tttgcttccc gttggtcaat
      841 tccttcaccc gttcggaaat ataattctcc aggccgctga cagttacctt tcctttattc
      901 ctcaagtctg cctgcccgct gagaccttca accaaggctt tggtgaacgc gccattaccc
      961 catctgctgt tttcgagcga ggcttgtcgc ccggtggaag cggtaaaaat aacggtccca
     1021 ttttcagccg ctcgtaattc ctggaccacc aggttgatgt cgggattggg cgcgccccgc
     1081 cggttggaac tgccaaagac attcccggag tgacaggtgt caacaaagag gaatttcttt
     1141 ccggccatac tgttgatggt ggttttgatg gaggaaaatg aaatcccggt ctttaacaaa
     1201 ttgttggtgt ccgcatcctg tggcaggtag taataggtat catcctcatc caccccatgc
     1261 cccgccagaa aaatcatggc gacatctttg gtggtggtct gggtttttat ccactgaaac
     1321 ccatccagaa tattgttatg agtggctgcg ctgtcggtca acaacctgac caccacatcc
     1381 tcataaagca atccccgttg ctgcttcaaa acccccacaa aatcggttgc gtccttggcc
     1441 ggatagttga gaatgagaga cgaatcagca tatgcgctga ccccaatccc caggatatag
     1501 agtttcggtt tgtattcatt gttttcaggc gcggtggtga caaggggttc ctgacaccag
     1561 cgcagactga ctgatgtttg cactttggag ccatcaggga gagttgccct cagcatcacc
     1621 tgtgtgtcat ggggcggaat gacaatctga agttccccgc cgccatcggc tccggctatg
     1681 gaggtagaag cggcaggggt tttcactcgc tgcccacgct cgaccatttg gttattgacc
     1741 caaacttcta atccggtttc cggtggagcg gaggtccgat accgcaacgt gagatattgc
     1801 gaacagaccg ttgaaccagc caagggggat aggatttcga tctggggtgt ggaaacccgg
     1861 gccgggccag agccggctgg ggttgtttgg tttcctgaag ccgttgattc gccagctagc
     1921 tgacggtacc gctgagcctg ttgcggttct ttcaaaatgt cataaatcag ggccagatag
     1981 tggtaacatt cagtatctgg ttgaaggctg accgctttct taaaatacaa caccgcttcg
     2041 tcatattttt tcgaatggta gtttaaaatt ccctgctttt tgacctcaat aaatgaaacc
     2101 tgggttttcc caggcaccga accgcaaacc atggcaggcc acaacaacat ggtggcacca
     2161 agaagagtgg ataccaaaag cctctggaat ttgaaaaagg aaagcatgtt tccctctgat
     2221 ggtgccagga aaattgagcc aggcaactca ctcaattttc ccgattaact tgcaagtcaa
     2281 agtattgatg ttcctggcta gccacggcgg cggcgattta tgggtttcgg gggggcaggt
     2341 ggaggcggtg ccggtttcgg tttgttattg ttggatttca tgacttcgac aatggcctca
     2401 ctcactcccc catctatcaa cttgaattgt tcctgaagcg acaaatcaaa ttgggttcgt
     2461 tcagcctgga caatcgcatt cctgataaaa tcagcggaga gctttcgctt gaccatcacc
     2521 aacacatcct ggttacgcaa caccccatct ccggataact gtttttccaa gggaggctcc
     2581 actttggccg cagtggcagc caaggcagca gcctcagcct cgtccatggc ggccttggtc
     2641 actttatatt ctctccaccc ggtacattcc ttttggctcc gcgaaatacg ttccataagg
     2701 aacccgctgg cagatgctcc ttcctgaaaa tatggttcag ccggattttt ggtacgagca
     2761 tcggtataat atgttaacgc ctgggctagt ttgggttccg ctgcctggaa atcct
//
LOCUS       DAOQDO010000643         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29518_length_2813_cov_2.801305, whole genome shotgun sequence.
ACCESSION   DAOQDO010000643 DAOQDO010000000
VERSION     DAOQDO010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29518_length_2813_cov_2.801305"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..2406)
                     /locus_tag="PKZ53_11045"
     CDS             complement(277..2406)
                     /locus_tag="PKZ53_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41017.1"
                     /translation="MAKQSKKKTELTIKDATTSAQPQKTTKSSATGFQTWLRPYQTEL
                     GIALGLLVCIGIVYGNVVTFGFVDFDDLQFVTANADIKKGFTLETVKWAFTTTYASNY
                     WMPVSWFSHILDWSLFGANAGGHHFTNVLFHAANTLLLFGLLRYLTGQAALSGVVAFF
                     FAVHPMQVEPVAWVAARKDLICTFFGFLTLWAYVWFAQPKRNWLAYGLTLVCFTLSLM
                     AKPLGIVVPPVLVLLDIWPLNRLPEPFRFSWPTLQSHILPLILEKIPFGLFALVIVFF
                     TRASGKVSGGSELQIRRPSTTLIWDGFSGYVYYIKKLLLPTNLAIFYDVPLTPYWLGL
                     LCLAVLVCVSGWVVLLSRHQRAVLVGWLWFGATLLPMVGFANLNTPRSDRYAYFSFIG
                     LFLIVVWGGQVWFQSNQAHKKMLQLGLGLVMLVCIGLSWAQTQVWKNNLTLFQHAVDC
                     GASNFVTESNLGVEYLKNNQLVEAEAHLKEAYRLDPSFVTTLSALGRIYEQRGQHAEA
                     IKYYEQALRKKPDMPDLLFNYGNALVNLNRGDEALEYLQEVLKHDPKHAKTYCALGTY
                     YFNKRMYPEAIQHYSKAIELQPDFFEARLNLANVLTSSKDPNTAIVHLNYALGLHPES
                     AIGFQILGNALNAANRPKDAIDAYQKSLHRDPNQADVHYRLAILLNSNGKQPDAIGHL
                     QTALKLKPGLTDAQNLLNSLQGAGKQG"
     gene            2622..>2813
                     /locus_tag="PKZ53_11050"
     CDS             2622..>2813
                     /locus_tag="PKZ53_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41018.1"
                     /translation="MSTVLPTTREIEVLAPQASLPAIRPKAEAPSRLEYGFRLLYVIW
                     VLAGFGLYLYCDLRGWLRKR"
BASE COUNT          719 a          686 c          714 g          694 t
ORIGIN      
        1 tcgggctgaa gaaggtgggc ttagggctga agactttggg cttggggctg aagacgtcgg
       61 gctgaagaaa atgggcttgg ggctgaagac tttgggcttg gggctgaaga cgtcgggctg
      121 aagaaggtgg gcttagggct gaagactttg ggcttggggc tgaagactcg caaactcggg
      181 gttgaaaaac ctgggttcaa tcccctgagc ccgatttctt cagcccgtct tcttcagccc
      241 gtctttttca gcccgatttc ttcagccctg ctttcttcag ccctgctttc ctgctccctg
      301 aagtgagttg agcaggtttt gggcatcagt taaccccggt ttgagcttca gcgcggtttg
      361 caaatgacca atcgcatcag gttgttttcc gttcgaattg agcaaaatag ccagtcggta
      421 atgaacgtca gcctgattgg gatcgcggtg gagtgatttt tgataggcat caattgcatc
      481 tttgggacga ttggccgcat ttaacgcgtt tcccaggatt tgaaatccaa tcgcggactc
      541 gggatggagc ccgagagcat agttcaaatg aacaatggca gtgtttggat ccttgcttga
      601 agtcagaaca ttggccagat taagacgggc ttcaaaaaaa tcaggctgga gttcaatcgc
      661 cttcgagtaa tgctgaatcg cttcggggta cattcttttg ttgaagtaat aagttcccaa
      721 cgcacaatag gttttggcat gttttggatc atgcttgagt acctcctgga gatattccaa
      781 cgcctcatcc cctcgattca ggttaaccag cgcattgcca tagttaaaca acagatcagg
      841 catgtcaggt ttttttcgga gggcctgttc ataatatttt atggcttcag cgtgctggcc
      901 tcgctgttca tagattcttc ccagagccga gagcgtcgtc acaaaagatg gatccagccg
      961 ataggcttcc ttgaggtggg cttcggcctc gacaagctgg ttatttttca agtattccac
     1021 acctaaatta gactcagtta caaagttact ggcgccacaa tcaacggcgt gctgaaataa
     1081 ggtcaggttg tttttccaaa cctgagtttg tgcccaggac aacccgatac agaccagcat
     1141 gacaagtcct aaacccagtt gcagcatttt tttatgagcc tggtttgact gaaaccacac
     1201 ttgccctccc caaaccacaa ttaaaaataa gccaataaat gaaaagtagg cataccgatc
     1261 agaacgaggc gtattgaggt tggcgaaccc aaccatgggc aacagtgtgg ccccaaacca
     1321 gagccagccg accaaaactg cccgttgatg acgactcaat aacaccaccc atccagagac
     1381 acacaccagc actgccagac acagaagacc aagccagtat ggagttaatg gaacatcata
     1441 aaaaatagcc agattggttg gtaacaagag ctttttgatg tagtagacat acccgctgaa
     1501 tccatcccaa atcaatgtgg ttgagggtct tcgaatctgg agttctgaac cgccagaaac
     1561 tttgccgctg gccctggtga aaaacacaat caccagcgca aagagtccaa atggaatttt
     1621 ctccagaatg agcgggagaa tgtgtgattg aagggtcggc caggaaaaac ggaatggttc
     1681 gggcaatcgg ttgagcggcc agatatcgag caacaccaga actggcggca ccacaatccc
     1741 caacggcttg gccatcagcg agagcgtaaa gcacaccagt gtcagtccat aagcgagcca
     1801 gttgcgcttt ggctgagcaa accagacata agcccagaga gtaagaaagc caaaaaacgt
     1861 gcagatcaaa tccttgcgtg cggcaaccca ggccaccggt tcaacctgca tcgggtgaac
     1921 cgcaaagaaa aaagccacca ctccacttaa agctgcctgc ccggtgagat atctcagcaa
     1981 cccaaataac aacaaggtat tggcggcatg aaagagcacg ttggtgaaat gatgaccgcc
     2041 agcatttgcc ccaaacaacg accagtccaa aatatgtgaa aaccacgaca cgggcatcca
     2101 gtagtttgaa gcataggtgg tggtaaacgc ccacttaact gtttccaggg taaatccctt
     2161 tttgatgtcg gcgtttgccg tcacaaattg aaggtcatca aaatcaacaa acccaaacgt
     2221 gaccacattg ccatagacga tgccgatgca aaccagaagg ccgagcgcaa tcccaagctc
     2281 tgtttgatat ggccgcagcc aggtctgaaa cccagtcgcc gaagatttcg tcgttttttg
     2341 cggttgcgcc gatgtcgtcg cgtctttgat tgtcagttcc gtcttttttt tggattgttt
     2401 cgccatattt ttagggttca gggttcaggg ttttcgaaga gggcgtgatt caaagcaacg
     2461 tcgcccttac accgggttgt cactttgtca ccctgtcacc ttgtcatttg ccaccgtgca
     2521 tcaggtacca gggtcggcac cttttcgtgg tcgtgctttg gtgctttttt tgttttttgg
     2581 tatagtggtg agtcctccaa gcattcaaag gaacgatcat catgtcaact gttttgccca
     2641 caacccgtga aatcgaggtc cttgcgccac aggcgtccct tcctgccatc cggcccaaag
     2701 cagaggcgcc gtcccgactg gagtatgggt tccgactcct gtatgtgatc tgggttttgg
     2761 ctggatttgg gttgtatttg tattgcgatt tgcgtggctg gctgcgaaaa cgt
//
LOCUS       DAOQDO010000644         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29558_length_2811_cov_3.477504, whole genome shotgun sequence.
ACCESSION   DAOQDO010000644 DAOQDO010000000
VERSION     DAOQDO010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29558_length_2811_cov_3.477504"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1228)
                     /locus_tag="PKZ53_11055"
     CDS             complement(92..1228)
                     /locus_tag="PKZ53_11055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ41019.1"
                     /translation="METTETPVGIMATSNAPASYPIEWMVEPYLHCEDGYLYNPLTDK
                     TLGIQEVGYHELRSVLDKEVAVAQVISLDLQLKLAEAGWLVPPSPRLDQRFRLKYVSL
                     EAHTVCNQSCFFCPVSVAPREAHFMPSEQYRDIVHQLVDYRDTIEAVFMLSYNEPTLD
                     RRFVEQVALLKANGLPPAINTNATGLTPQRVDALLEMGGVRYLSVNLSTLSASRYEHD
                     RGKDQLDLVLRNLDYLKDLPVADQMDVAVLGTGDLTHKFEFWRISHHFRGSRLQVKFF
                     KVMDRAGFLDVGLKVVSTSVRLCGCENVGSRPLQHLHITPHGQCVMCCQDYQARHLVG
                     DLNRQTVAEVLVGPEMAQVRRWTYGLDDAPADFLCRRCIFARIR"
     gene            complement(1215..>2811)
                     /locus_tag="PKZ53_11060"
     CDS             complement(1215..>2811)
                     /locus_tag="PKZ53_11060"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41020.1"
                     /translation="RVRFKVMLEGYDRNWIETGDQRRVSYTNLPPRSYQFRVLACNND
                     GVWNETGAAFRFKIRPYWYQTPWAYLAGLALVAGSVYGGVRLRLRALNRRAAQLEAVV
                     VERTTELATKNSELDRKNAELAENIEQVRLAKLETERKNTELDQKVAALDRANQELVI
                     SHQQADRIFSALAEALPGTVLEGKYRLEEKIGSGGFGAVFRGTHLVLNRPIAVKVFRP
                     APGNDSADAVERFRLEGVSSSRINHPNAIQVFDSGISAEGIAYLVMELLDGCSLSEEL
                     QTRKRFSVRRTLAVIRAVCEGLAEAHRLGIIHRDIKPANIFLHRSPEGEVVKVVDFGV
                     AKMLDDPEASGKKLTMTGGIVGTPAYMAPERLLDSQYDGRSDIYSVGIVFYECLCGRP
                     PFVSEGGSAYQVIMAHMNELPPSTRSINPTIPPEVDEAVLKSLVKRPDERPTAREFVD
                     LLTTLAEQFAEYDADAEIDFLPQRLADQPTVGLDSLHPTLDMPLAEQITQTGTVPSRS
                     TSETSLSDQPTAEIVNPYSDGNN"
BASE COUNT          667 a          787 c          725 g          632 t
ORIGIN      
        1 gaaccctgaa ccctgaaccc tgaaccctga accctgaacc ctgaaccctg aatcctgaac
       61 cctgaagacg gcgaaaacgc tgtcatcttc cctatcggat gcgggcaaaa atgcaccgcc
      121 ggcagagaaa atcggctggt gcgtcgtcaa gaccataggt ccaccgccgg acctgggcca
      181 tttccggacc aactaaaact tcggcgactg tttggcgatt gagatcaccc accagatggc
      241 gagcctgata gtcctggcag cacatcacac actgaccgtg cggtgtgata tgtaaatgtt
      301 ggagcggacg cgagccaaca ttttcacaac cgcatagtct gactgaagtt gagacgactt
      361 tgagtcccac atccagaaac ccagcgcgat ccatgacttt gaaaaatttg acctgcaacc
      421 gtgagccacg aaagtggtgt gaaattctcc agaattcaaa tttgtgggtg agatcaccag
      481 ttcccaaaac tgccacatcc atctggtcag caaccggtaa atctttcaaa tagtcaaggt
      541 tgcgcagcac aaggtcaagc tggtctttgc cccggtcgtg ttcataccgg ctggcactca
      601 aggtcgaaag gttcaccgaa agatatcgta cgccgcccat ttcaagcagg gcgtccactc
      661 gctgaggcgt caaccccgtg gcattggtgt tgatggctgg cggaagccca tttgctttaa
      721 ggagtgccac ttgctcgaca aatcgccggt caagggtcgg ttcgttgtaa ctcagcataa
      781 agaccgcctc aatcgtgtca cgatagtcca caagctgatg cacgatgtca cgatattgct
      841 cagatggcat aaagtgggct tcacgcggag cgaccgagac cgggcagaaa aaacacgact
      901 gattgcacac cgtatgggct tcgagcgaca cgtacttgag tcgaaatcgt tggtcgagtc
      961 ggggagaagg cggcaccagc cagccagctt ctgccagctt gagctgcagg tccagcgata
     1021 taacttgagc aacagcaacc tctttatcca acacactccg gagttcgtga tacccaactt
     1081 cctgaatccc caacgtcttg tcggtcaatg ggttgtacag ataaccatct tcacagtgaa
     1141 ggtatggttc aaccatccac tctattggat atgacgctgg ggcatttgaa gttgccataa
     1201 ttcccaccgg ggtttcagtt gtttccatct gagtacgggt tgacgatttc ggcagtcggt
     1261 tgatcactca gggacgtttc tgaagtcgaa cgcgagggta cagtaccggt ttgggttatc
     1321 tgctcagcca gtggcatgtc cagtgttggg tgcaagctgt cgagaccaac ggtgggctga
     1381 tcggccagtc gttgagggag aaaatctatc tcggcatcag catcatattc ggcaaattgc
     1441 tcagccagtg tcgtcaataa atcaacaaac tcgcgggctg ttggacgctc atctggtcgc
     1501 ttgaccagtg atttgagaac ggcttcatct acttcgggcg gaatggttgg attgattgag
     1561 cgggtcgaag gcggcagctc attcatatgc gccatgatga cctgataggc gctgccaccc
     1621 tcgctgacaa atggcggacg accgcataaa cattcgtaaa acacgatccc cacgctataa
     1681 atatctgacc gtccatcata ctgactatca agcaaccgtt cgggtgccat ataggccggg
     1741 gtgccgacaa tgccgccggt cattgttaat ttcttgccgc tggcttcggg gtcatcgagc
     1801 attttggcga ctccgaaatc caccactttg acaacttcgc cttcgggtga ccggtgtaag
     1861 aagatattgg ctggtttgat gtcacgatga atgatcccca gacggtgggc ctcagccagt
     1921 ccttcgcaca cagcgcgaat caccgccagg gtacgtcgca cactaaaccg ttttcgagtc
     1981 tgaagctcct cgctgagcga acatccatcg agcaactcca tcaccagata agcaatgcct
     2041 tcagccgaaa ttcccgagtc aaacacctga atggcattcg gatggttgat gcgcgacgaa
     2101 gaaacacctt ccaggcggaa ccgctcaacc gcatcagccg aatcgtttcc tggcgccggg
     2161 cgaaagactt tgaccgcaat cggacgattg agcaccagat gtgttccacg aaacacagcc
     2221 ccaaatccac cagagccgat tttttcttca agccggtact tcccttccaa aacagttcct
     2281 ggcagggctt ctgccagggc cgagaaaatc cggtccgcct gctgatgaga aatgaccagc
     2341 tcctgattgg cccggtcgag tgccgcgact ttctgatcca gttcggtgtt tttgcgctcg
     2401 gtttcaagct tggccagtcg aacctgctca atattttcag ccagttcggc atttttgcgg
     2461 tcaagttcgc tgtttttggt cgccaactca gtcgtgcgtt ccaccacaac ggcttcaagc
     2521 tgggctgctc gccggttgag cgcccggagg cgcaaccgaa ctccgccata cacgctgccg
     2581 gcaaccagag ctaagccggc cagataggcc cacggcgttt ggtaccaata gggacggatt
     2641 ttgaacctga acgccgcccc ggtttcattc cagacaccgt cgttgttgca ggccaggacc
     2701 ctgaactggt aggaacgtgg cggcaagttg gtgtaactga cccgtcgttg gtcgccagtt
     2761 tcaatccagt tccgatcata cccttcgagc attaccttaa atcgcaccct g
//
LOCUS       DAOQDO010000645         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29586_length_2810_cov_2.850091, whole genome shotgun sequence.
ACCESSION   DAOQDO010000645 DAOQDO010000000
VERSION     DAOQDO010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29586_length_2810_cov_2.850091"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(452..772)
                     /locus_tag="PKZ53_11065"
     CDS             complement(452..772)
                     /locus_tag="PKZ53_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41021.1"
                     /translation="MQKLGRKTFKQIVAEVAPINIRRLIAKAILANRIAKLQMIRGRS
                     QAYRLKGSVLWKLQRLVPSRVFPDSERTSADILVFVIPRYRFQIHIGSWQLSPPGQPD
                     DVNY"
     gene            1230..2792
                     /locus_tag="PKZ53_11070"
     CDS             1230..2792
                     /locus_tag="PKZ53_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41022.1"
                     /translation="MPKKKKQTRQANAEELAQLKKIIDDISHSADQKHLVSEARKLVR
                     QFEQQANSVWDHLLTDLEKTFFDYSELQTLIHTVREKTHTKSDATRQPTNSAKLDDLL
                     KYPANSIAKATVEALLPRKLMENQYFNGDTFWKAYPFQAGVRTKNGEINGLIGYRSPA
                     EAIWEYLQRTGPLAIQVQFALWSKAYGCTGGTPGQFVTVNINQFLDELGYSRKKGSHK
                     RETREAVVAALEAVTSLEIEAEWYARDGRHYQFRGPLWLRGGTIRIAGEQGDLFRKIN
                     QPQWDPYTFEYAPGRLFENAEWRKYNAEIAYVGEGLLQLTAENRDRNAVYIGGYLATL
                     ARMNGYRRSILKASTLAEKSALNESYPNEPSRIIEKLTLALNRLVKVKVIRKWQWLKE
                     IQDDINFDDFDSYETLDRLREGEAWRLRGDQAVEIIWPERLQVLGTRLEERKETRIKA
                     ERKRGRPAKTPIPEPSISASQPESQGPFNQLPLFSNEPPKRKRGRPPKAISQVLIPQI
                     PKRPRGRPKKEK"
BASE COUNT          834 a          687 c          593 g          696 t
ORIGIN      
        1 ttgagagcaa attctaattc ttctagctga tccaaaatcc ctttcagaag ctgccgtgga
       61 ctgttttcaa agcccaagga atgcggttcc cccgggagtg agaaacaccc tcaaaatagt
      121 ctcggaggtg cttccgtgcg ttatgccgat acaataatcg cagcccttcc acgattttcc
      181 cggaactcaa cttctgctcg gcctgtatct gcccaacaag ctgatgcaaa agcaatgccc
      241 aactgcttat tgtttttcga tttgtcaatc cgccaaataa ccgccggata atccgatgtt
      301 gatttccccg ttcatttgtt cgaacagact cgataaccac ccaaattacc attcccacaa
      361 tcatcaaaat ccccacactt atcatcttct tgctcgactt tcttgagagc ctgtcttctg
      421 aagagactcc atatagtttg agtcagaagt tctagtagtt gacatcatcc ggctgcccag
      481 gcggtgataa ctgccagctt ccgatatgaa tttggaatcg gtatcgagga atgacaaaaa
      541 ccagaatgtc cgctgaagta cgctcgctat caggaaaaac ccgtgagggc acaagtcttt
      601 gcaacttcca caacactgaa cctttcaacc tgtaggcttg acttcgacct cggatcatct
      661 gcaacttggc aattcggttc gcaagaatgg ctttggctat caaccggcga atattgatcg
      721 gagcaacttc agccacaatt tgtttgaacg tctttcgtcc aagtttctgc ataccctcct
      781 ccctggattg tgatttgcta cctgatcgcc catagatagc tcgggtttca atcaatagtt
      841 cacttcaccg cgaggttttt ctggactgct gcatttccca acagtggcaa aggctatttc
      901 cttagcaact tgtagcccaa ctgagcctca ctggacctca tattggggac attggggaca
      961 ttggagacat gcgacattga actatcaaaa gtggacaacc acgtaccttc aggttcacca
     1021 acaatcttga agttgatttg atatcaaaat agtattaaaa aggtactata tcagtacata
     1081 aaatatacaa atatgaattt atcttaatat ttatatagta ctggaagagt attatcatgt
     1141 ttcagtttgg tcagttccct tattaccttc accaaccact gacatatcag gcataatgcc
     1201 tcgtttgaga ttgtcaggga gtcattcata tgccaaagaa gaaaaaacaa accaggcagg
     1261 ccaatgcgga agaactggct caactcaaaa aaatcattga cgacatcagc cactctgcag
     1321 atcaaaaaca cctggtatct gaagctagaa aacttgtccg tcagtttgaa caacaggcaa
     1381 attcggtgtg ggatcatttg ctcacagatc tcgaaaagac gttttttgac tattctgaac
     1441 tccaaacact gattcacact gtcagagaaa aaacacatac gaaatctgat gcaacgcgtc
     1501 agcccacaaa ctccgccaaa ttagacgact tgctcaaata ccccgccaac agtattgcca
     1561 aagcaaccgt tgaagcgctt ttacctcgta aactgatgga aaatcagtat ttcaatggtg
     1621 atactttttg gaaggcatat ccatttcagg ctggtgttcg cacgaaaaac ggagagatca
     1681 atgggttgat agggtaccga tctccagcag aggcaatttg ggagtacctt caacgtacag
     1741 gaccgctcgc cattcaggtc cagtttgcgc tctggagtaa agcgtatggc tgtacggggg
     1801 gtacccccgg acaattcgtc accgtcaaca tcaaccagtt tctcgatgaa cttggctatt
     1861 cgcgtaaaaa aggaagccat aaacgcgaaa ctcgtgaagc ggttgttgcg gcgcttgagg
     1921 cagttacatc acttgaaatc gaagccgaat ggtatgcccg agatggacga cactaccagt
     1981 ttcgaggacc actttggctt cgcggaggca caatccgaat tgcaggtgag caaggggatc
     2041 tgttcagaaa aatcaaccag cctcagtggg atccttacac cttcgaatat gcccctggaa
     2101 gactctttga gaacgctgag tggcgcaaat acaacgctga aattgcctat gtcggtgaag
     2161 gattgctcca gttgacggct gaaaaccggg atcgaaatgc tgtttacatc ggtggatatt
     2221 tagccacact tgcccggatg aacggatatc gccgcagcat tctcaaagcc agcacccttg
     2281 ctgaaaaaag cgctctcaat gagagttatc caaatgaacc cagccggatt attgaaaaat
     2341 taaccctggc cctcaaccga cttgtcaaag tcaaagtcat tcggaaatgg caatggctca
     2401 aagaaattca ggatgatatt aattttgacg attttgactc gtatgaaacg ctggatcggc
     2461 tgcgcgaagg tgaagcctgg agacttcgtg gggatcaggc agttgaaatt atctggccgg
     2521 aacgattaca ggttttaggt actcgacttg aagaacgaaa agaaacgcgt atcaaagctg
     2581 aacgcaaacg tggacgacca gccaaaacac caatccctga accatccatt tcagcaagcc
     2641 aaccagaatc tcaaggtcca tttaatcagc tcccactttt ctctaatgag cccccaaaac
     2701 gaaaacgcgg aagacctccc aaggcaattt ctcaggtgct aatccctcag attccaaaac
     2761 gtcctcgtgg aagaccaaaa aaagaaaaat aatccgaaaa ttcaggtaaa
//
LOCUS       DAOQDO010000646         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29616_length_2809_cov_2.659768, whole genome shotgun sequence.
ACCESSION   DAOQDO010000646 DAOQDO010000000
VERSION     DAOQDO010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29616_length_2809_cov_2.659768"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKZ53_11075"
     CDS             complement(<1..372)
                     /locus_tag="PKZ53_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HNJ41023.1"
                     /translation="MASNGDEVEKAMKSIRAETLRAHVRFLADDLLEGRGPGTRGAEL
                     AARYIASQFETYGLEPAVNGGYYQNVPIVNIKAEAPTAKMEAAAGGKKEVLTYGEEFT
                     VQSGLGETDVDIHRAEVVFVGY"
     gene            744..1775
                     /locus_tag="PKZ53_11080"
     CDS             744..1775
                     /locus_tag="PKZ53_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ41024.1"
                     /translation="MELFESQKQLDEGNRKITRRVFVGVIVGGVAAAIGKRFYDDLDK
                     PPSGIKTDFISQIGEFYTIYFNEYPTNLTTDGWSMRFAGPEGKSFSLDYNGLKALPNK
                     LIYKTFECIENGVGENIINNAEWRVTPLQPLLDRILPTEFSSLNVIFRAYDGYYTGLP
                     IADVYTEESYLAYEMNGQPLPLQHGRPARVLLPGKYGYKQPKWLKEIEVTAGPLVGYW
                     ESKGWAVSGEVKPMSRIDTVGYDPKPAGDMPGDALITGIAYGGRFAVSKVEVSLDNGS
                     SWQPAEVTSPTGPNHWSLWKYVWPNAPRGRHKILVRMTDANGKLQPEARKGYYPAGSS
                     GWHRVVVKL"
     gene            complement(1898..>2809)
                     /locus_tag="PKZ53_11085"
     CDS             complement(1898..>2809)
                     /locus_tag="PKZ53_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNJ41025.1"
                     /translation="VVGLDGCSSFRGKSGNAPEGFSTAPASPSPASKRVSQPGLAVQS
                     DTISTDPQVPNAPATTVEPQSRLIIRNAELSLEVDSPSEAMRRVTQIAEAQKGFVVTS
                     ESREVRSSQTQSSQIVTMVFRVPSATFGDTLEKVRQVGQRVLQEKVTGQDVTEEFLDL
                     EARIRSKKALEAQFLEIMKTARTVSDALEVQRQLAEVRTQIEQLEGRRRYLENQSALS
                     TFTVTLNEPSPIVTTSPTGFLQEVKQTIADSIDLAANIVLGIIRLVILLIPISILIFL
                     PFGLLIRWFIRRFFRSRKAISEAVKTE"
BASE COUNT          683 a          682 c          747 g          697 t
ORIGIN      
        1 atagccaaca aaaacaactt cggcccggtg aatatcaaca tccgtctcac ccagaccact
       61 ctggacggta aattcttcgc cgtaggtaag tacttctttt ttgccaccag ccgcggcttc
      121 cattttggcg gttggggctt cggccttgat gttgacgatg ggaacattct ggtaataacc
      181 accattgacg gctggttcta atccgtaggt ttcaaactgt gacgcgatat agcgagccgc
      241 aagctctgcc ccacgtgtac cggggccacg gccttcgagc aggtcatcag ccagaaagcg
      301 aacatgcgcc cggagcgttt cggcacgaat tgatttcatt gctttttcaa cttcatcgcc
      361 attgctggcc agaacgtggg cgacgcccga tggtaacgtt gtggtcagac agacaaacag
      421 ggcaaaaaat actgtcagcc ggcgaagaat gggggaaacc gggttccggg ttccgggttc
      481 cgggttccgg gtttcagaac ctgaagcttg atttttgagg acattccttt gtgtcacggg
      541 caaactctcc ttctcagtgg aatttaggac tggggttgag ggttcagggt caggtatttt
      601 gaatgtttgg cctttttatc tgttcttgct gaaaacccgg aacccgaaag attcggtgaa
      661 attgattgtg agcggcctga aactctgaca atactcagtt tgtcagcggg aacgcaacct
      721 gaccgaagtg gagcacgcaa ccgatggaat tatttgagtc acaaaagcag ttggatgaag
      781 gaaatcgcaa aataacccgc cgggtttttg tcggtgtgat tgtgggcggt gtggcggcag
      841 cgattggaaa acgattttat gatgatctcg ataaaccgcc gagcggaatc aaaaccgatt
      901 ttatttcgca gattggcgag ttttacacga tttatttcaa tgaatacccg accaacctga
      961 caacggatgg ctggtctatg cggtttgctg gtccggaggg aaaatcgttt tcgttagatt
     1021 acaatgggct caaggctctt ccaaataaac tgatctataa aacctttgaa tgtatcgaaa
     1081 acggcgttgg tgaaaacatc atcaacaatg cggaatggcg cgtcacaccg ctccaaccgc
     1141 tgctggatcg cattctccca actgaatttt caagcctgaa tgtgattttc cgcgcctatg
     1201 atgggtacta caccggattg ccgattgccg atgtctacac cgaagaaagc tatctggctt
     1261 atgaaatgaa cggccagccg ttgccgctcc aacacggacg accagcccgc gtgctcttgc
     1321 caggaaagta tgggtataaa caaccaaaat ggcttaagga aattgaagtc accgccggac
     1381 cactcgtcgg atattgggaa agcaaaggct gggcagtctc gggcgaagtc aaaccgatgt
     1441 ctcggattga caccgtcggc tatgacccaa aaccggcagg cgacatgcca ggagatgcac
     1501 tgattaccgg gattgcctac ggcggcagat ttgccgtgtc taaagtcgaa gtcagcctgg
     1561 ataatggctc aagctggcaa ccggctgaag tcacatcacc aaccgggccc aatcactggt
     1621 cactgtggaa atatgtctgg cccaatgccc cacgcgggag acacaaaatt ctggtgcgaa
     1681 tgactgatgc caatggaaaa ctgcaacccg aagcccgcaa aggctattat ccggcggggt
     1741 cttcgggctg gcatcgggtg gtggtgaagc tgtaggaatt ggggttcagg gttcagggtt
     1801 cagggttcag ggttcagggt tcaggggttt cgaattttgg tcctttttgt cctttctgtc
     1861 cttttcgtct ttttgttggc cagaacctaa cttgccatta ttcggttttc acagcctcag
     1921 aaattgcttt ccgtgaccgg aaaaatcgcc ggatgaacca tcgaatcagc aatccaaacg
     1981 gcaaaaaaat caaaatactg attgggatga gcaagatcac cagccgaata atccccagca
     2041 caatatttgc agctaaatca atactatcag cgatagtctg tttaacctct tgtaaaaatc
     2101 cagtcgggct ggtggtgaca atcggcgatg gctcattgag cgtgacagtg aacgttgaca
     2161 gggctgactg gttttccagg tagcgccgac gtccctcaag ctgttcgatt tgggttcgta
     2221 cttctgcaag ctggcgctga acttcgagcg cgtccgacac ggttcgggcg gttttcataa
     2281 tttccagaaa ctgggcttca agggcttttt tgctgcggat gcgggcttcg aggtcaagaa
     2341 attcttcggt gacatcctgt cctgtcactt tttcctggag tacacgctgc ccaacctgac
     2401 ggactttttc aagtgtgtca ccaaacgtcg ccgacggcac ccggaaaacc attgttacaa
     2461 tctgactgga ttgggtttga ctgctgcgaa cctctctgga ttcagaagtt acgacaaatc
     2521 ctttttgggc ttcggcaatc tgagtcaccc ggcgcattgc ctcggaagga gaatcaacct
     2581 caagagataa ttcggcattg cggatgatca ggcgtgattg gggttcaacg gttgtggctg
     2641 gggcatttgg tacctgagga tcagtactga tggtgtcact ttgaaccgca agccctggtt
     2701 gagacacccg tttactggct ggtgatggtg aagccggggc cgtactgaaa ccttcggggg
     2761 cattcccact cttccctcta aatgaagagc acccatccaa tcccactac
//
LOCUS       DAOQDO010000647         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29663_length_2806_cov_2.968739, whole genome shotgun sequence.
ACCESSION   DAOQDO010000647 DAOQDO010000000
VERSION     DAOQDO010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29663_length_2806_cov_2.968739"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PKZ53_11090"
     CDS             complement(<1..1391)
                     /locus_tag="PKZ53_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ41026.1"
                     /translation="MNPESLISKGQERLAALEQYGLLGELASDATFDRVTGLAVRLFQ
                     VPLAIICFVGQDRKQIISRAGTDPGSVESEVGFCGLTVDTDSPVVLTDPAHTAVTLGI
                     MLPPDFPFPIGFYVGVPLRLPNGLAVGTLSLIDQHTRVFSETDLASLIDLALVASDHL
                     ALKIVTQSLSRTEEILKQHSRVLVQLSSREAAHRGELTAELQAITEVAAQTLKVDRCG
                     IWLLNDDQTQLISQDMYNWRTLMHGQNPNLLTTNYPHYVKALAEERIIAASDAVRDFR
                     TQEFTPGYFLPHKVASTLDAPIRLGGQIIGVICLEHCGQIRHWTIEEQQFAGSLADLA
                     ALAVEASERREAEEALRKSEEHYRKLFENADDIIYSHDLTGKILTANGATERLIGYTV
                     PEVLTKNIFDLVAPEYQLPIRKTMSPRRVRPESPTNNTSLVTFFQFEVLTKDGRRTWV
                     EANTQPIIEGNRIV"
     gene            complement(1743..>2806)
                     /locus_tag="PKZ53_11095"
     CDS             complement(1743..>2806)
                     /locus_tag="PKZ53_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ41027.1"
                     /translation="TKEAANKQGVEQVTLDELFARADFITVHTPYTPETANLLNRTAF
                     SKMKRGVRIVNCARGGLIDELALVDAIKEGIVAGAALDVFENEPPPADHPLLKLDQVI
                     YTPHLGASTQEAQVSVAVAIAHQMVDFFRTGAVFGAVNAPSVSAEILTEVGPYITLGE
                     KLGSFQGQAFGHNLKRVQIEYSGNVAGFDVRPIAQAILVGLLGPTSDRINFVNALLIA
                     EERGITVTESKQHKSTDFASLITITAETTDQESTIAGALFGQKDLRIVRVNGFPLEAI
                     PQGNMLLCTNRDLPGVLGRICARLGEEHINIARLYLGRKEIGGKAILLAQVDSELSDE
                     VITALNQVPDVLSARKIQL"
BASE COUNT          664 a          696 c          783 g          663 t
ORIGIN      
        1 acaatgcggt tgccttcaat gatgggctgg gtgttggcct cgacccaggt ccggcgacca
       61 tctttggtta aaacttcaaa ctggaaaaat gtcaccaggg acgtgttgtt ggtgggtgac
      121 tcgggtcgaa cgcgcctggg cgacatcgtt ttgcgaatcg gcaactgata ttcaggggcc
      181 accaggtcaa aaatattctt ggtcagtact tcaggcacgg tgtacccgat caagcgttcg
      241 gtggcgccat tggccgttaa aatcttcccg gttaaatcgt ggctgtaaat gatatcgtcg
      301 gcattttcaa agagtttgcg atagtgttct tcgcttttgc ggagggcttc ttcggcttca
      361 cgtcgttccg aagcttccac ggccagagcg gccagatcag ccagcgaacc agcaaattgt
      421 tgttcttcaa tcgtccagtg gcgaatctgg ccacaatgtt caagacagat gacaccgata
      481 atctgtccgc ctaaccggat gggggcatca agggttgagg ccactttgtg cgggagaaag
      541 taacctggag taaattcctg tgtccgaaaa tctcgaacgg cgtcactggc ggcgataatt
      601 cgttcttcag ccagagcttt gacatagtgc ggatagttgg tcgtgagcag atttgggttc
      661 tggccatgca tcagcgttcg ccagttgtac atatcctggc tgatcaactg ggtttgatcg
      721 tcgttgagaa gccagattcc acaccgatca accttgagtg tttgggccgc gacctcggta
      781 atggcctgca gctcagccgt caactcacca cgatgggccg cctcacggct ggaaagctgg
      841 accagaaccc ggctatgttg cttgaggatt tcttcagttc gactcaagga ctgagtgaca
      901 attttgagcg ccaggtggtc tgacgccacc aatgccagat caatcagcga tgccagatcg
      961 gtttcagaaa agacgcgggt gtgctgatca atcaaactca aggtgccaac cgccaggcca
     1021 tttggcagtc gaagcggaac acccacataa aacccaattg gaaatgggaa gtccggggga
     1081 agcataatcc ccagggtgac agcggtgtgg gccggatcgg tcaacaccac tggggaatcg
     1141 gtgtcaaccg tcaatccaca aaacccaacc tctgattcaa cagagccagg atctgttcca
     1201 gcccgcgaaa taatttgttt tcggtcctgt cccacaaaac aaatgatggc caggggcacc
     1261 tgaaataacc ggacagccag accagttacc cgatcaaagg tggcatctga cgccagttcg
     1321 cccagcaatc catattgctc aagcgccgcc agtcgctctt gtccctttga aatgagtgat
     1381 tctggattca tagcgtaaat cctggtcagg tagatatccc ggcgtggatg gtgggcagag
     1441 gtgttgccgg aggagaacct gggtcgttcg caatcagact gggaaacggt ttcggatgaa
     1501 attatggaag tatccagcca atgacaagcg acttacttac ctggctgaag cctgcttttc
     1561 tgattattca ataatatggc gtgagggaat ttgcattcat tcgccaacat ctaaccacgg
     1621 acaaggtggg aaggctgaac ctgatgaggg gagagggttg gggttcgatg ttgatggttg
     1681 aatcgcaaac tcgccgaagg tgtgggtgac gagtttgcga ttattgctga aatagaagtg
     1741 gtttacaact gaatctttcg tgctgaaagc acatcaggca cctgattgag cgccgtgatt
     1801 acttcatctg acagttcact gtcaacctgg gccagcaaaa tggctttgcc gccaatttct
     1861 tttcggccaa ggtacagccg ggcgatattg atgtgttctt cacccagtct ggcacaaatc
     1921 cggcctaaca cgcctggcag gtcacgattg gtacacagga gcatattgcc ctgggggatg
     1981 gcttcgagcg gaaagccgtt gacccggacg atgcgcaaat ccttttgtcc aaatagcgca
     2041 ccggcaatgg tgctttcctg atccgtggtt tcagccgtaa tggtaatcag gctcgcaaag
     2101 tcggttgatt tgtgttgttt ggattcggtg accgtaatgc cacgttcttc agcgattaac
     2161 agggcattga caaagttaat ccggtcactg gtcggaccta acaacccaac cagaatggcc
     2221 tgggcaatgg ggcggacatc aaacccagcc acatttccgc tatattcaat ctgaacccgc
     2281 ttgaggttat gcccaaacgc ctgaccctgg aaggagccaa gtttttcgcc cagcgtgata
     2341 taggggccga cttcggtcaa aatttcggca ctgaccgatg gggcattgac cgcgccgaaa
     2401 acggcacccg tccggaagaa gtccaccatc tggtgagcaa tggccacggc tactgaaacc
     2461 tgtgcttcct gggtggaggc gcccaggtgc ggtgtgtaaa tgacctgatc aagcttcaag
     2521 agcggatgat cagccggcgg tggttcattt tcaaagacat ccagtgcggc gccagcgaca
     2581 atgccttctt tgatggcgtc aaccagtgcc agttcgtcaa tcaggccgcc acgggcacag
     2641 ttcacaattc ggacgccgcg tttcattttt gaaaaagcgg tgcggttgag aaggttcgct
     2701 gtctcagggg tgtatggggt atgaacggtg ataaagtctg cgcgggcaaa gagttcatca
     2761 agggtgacct gctcaacgcc ctgtttattg gcggcttctt tggtca
//
LOCUS       DAOQDO010000648         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29666_length_2806_cov_2.818611, whole genome shotgun sequence.
ACCESSION   DAOQDO010000648 DAOQDO010000000
VERSION     DAOQDO010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29666_length_2806_cov_2.818611"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2602)
                     /locus_tag="PKZ53_11100"
     CDS             complement(<1..2602)
                     /locus_tag="PKZ53_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ41028.1"
                     /translation="MCILVLCLTQVVLASAQQSNPIKLDSGSFGAVEARSIGPATMSG
                     RISYIDGVNSNPLILYVGTASGGVWKTVNGGTTFQPIFDKHAFSIGAIAVDQSKPDTV
                     WVGTGESWVRNSVSVGTGLYKSTDAGETWQMMGLEKTERIGKIVIDPKNPDVVYVAAL
                     GHLWDGNEDRGLYKTTDGGKTWNKMLYVNADTGCSDVAIDPQEPSIVYAGMWQFRRYP
                     WSFNSGGPGSGLHKSTDGGKTWAKLTKGLPEGDLGRIALAVAKNRPNMVYACVEAKKT
                     GLYRSDNLGETWELMNSSFTTSVRPFYFSHLVADPKDYKVVYKAGLIMAVSKDSGKSF
                     TNLGGSSHGDQHTIWIDPNNTAHLYVGTDGGVYESRDGGRSYNFLRNLPVSQFYHVTY
                     DLEAPYNVYGGLQDNGTWVGPSRSPGGIQIKDWDSIGFGDGFWVFPDPADASLVYVES
                     QGGGLQRLYRKTGEFKDIKPRPEAGDPELRFNWNTPIEVGAKNQKQLFIGSQFLFQST
                     DRGESWQKISPDLTTNNPEKLKQEESGGLTIDNSTAENHCTIYSISSSPVDENVIWVG
                     TDDGNVQVTRDDGKTWANVTQNIPNLPKNTWVSCVEASHHAAGTAYATFDGHNTGDMK
                     AWVYQTTDFGQTWKPIMTDDIKGYAHVVREDLVNPNLLFVGTEFGLFISVTGGTQWAP
                     LTGKFPAVAVRDLSIHPRESDLLIATHGRGIYIVDDITPLRKLTNDVLNSEVVVLDGR
                     PSVMRFGGAIQEFPGDDEFAGPNATDSAIITYYLRERHVVGDFKVEVFDPQGKLVSTL
                     PGSKRRGINRVEWPMRLKPPKAIPSSAALGGTPPGAFVGPIAPEGTYTVKITKGQKTF
                     DGKFQLVPDPR"
BASE COUNT          668 a          750 c          707 g          681 t
ORIGIN      
        1 accgtgggtc gggaactaac tgaaacttcc catcaaatgt tttctgcccc ttggtgattt
       61 tgaccgtgta ggttccttct ggcgcaattg gtccgacaaa cgcacccggc ggcgtgccgc
      121 ctaaggccgc cgaacttggg attgccttcg ggggcttaag ccgcattggc cactcgaccc
      181 ggttgattcc ccgtcgtttt gaaccgggga gcgtcgaaac cagttttcct tgtggatcaa
      241 aaacttcaac cttgaaatct ccgaccacat ggcgttcccg cagataatag gtaatgatcg
      301 ccgaatcagt cgcgttgggc ccggcaaact cgtcatcgcc tggaaattcc tgaatcgccc
      361 caccaaatcg catcaccgaa ggtcggccat ccagcacaac aacctctgag ttcagaacat
      421 cattggtcag tttgcgcaac ggtgtgatgt catccacgat atagattccg cgtccgtgcg
      481 tggcaatcaa caaatcggat tcccgtgggt ggattgagag atcgcgaacg gccacggcgg
      541 ggaatttccc ggtcaggggc gcccattggg tgccaccggt gacactgata aacaggccaa
      601 attccgtccc gacaaacaac aagtttggat tgaccagatc ttctcgaaca acgtgggcat
      661 agcccttgat gtcgtcagtc atgatcggtt tccaggtctg gccaaaatcg gttgtttgat
      721 agacccaggc tttcatatcg ccagtgttat gtccgtcaaa ggtggcatag gccgtaccag
      781 cagcgtgatg actggcttcg acacaactca cccaggtatt tttgggaagg ttgggaatgt
      841 tttgggtcac attggcccag gtctttccgt catcgcgagt gacctgaaca ttgccgtcat
      901 cggtgccaac ccagattaca ttttcatcca ccggcgaaga actgatgcta tagatggtgc
      961 agtggttttc agccgttgaa ttatcaatgg tcagtccgcc ggattcttcc tgtttgagct
     1021 tttcagggtt gttggtggtc aggtcaggtg aaattttctg ccaggattcg ccacgatccg
     1081 ttgactggaa caggaattga gacccgatga agagctgctt ctggtttttg gcaccgactt
     1141 caatcggggt attccagtta aagcgcaatt ccgggtcacc ggcttctgga cgtggtttga
     1201 tgtccttgaa ttcgcctgtt ttccgataga gccgctgcag gccgccgccc tgtgattcaa
     1261 catagaccag tgaggcatct gctggatccg gaaacaccca aaacccatcc ccaaacccga
     1321 tgctgtccca gtctttgatt tgaataccgc cggggctgcg cgaagggcca acccaggtgc
     1381 cattgtcctg caacccgcca tagacgttgt atggggcttc caggtcatag gtcacgtggt
     1441 aaaactgtga cacgggcaag ttacgcagga aattataact tcgccccccg tcacgtgatt
     1501 catacacgcc accgtcggtc ccgacataca ggtgggccgt attatttggg tcaatccaga
     1561 tcgtgtgctg atcgccgtgg gaactgcccc ccaaattggt aaatgatttg ccgctgtctt
     1621 tactcaccgc cataatcagc ccggctttgt agacaacttt ataatctttg ggatcagcca
     1681 ccagatgcga aaaataaaac gggcgcaccg aggtggtaaa tgacgagttc atcaactccc
     1741 aggtctcacc cagattatct gagcgataca gccccgtctt tttggcttcg acacaggcat
     1801 agaccatgtt ggggcggttt ttggccacgg ccagagcaat ccgtcccagg tcgccttcgg
     1861 gcaacccctt ggttaatttc gcccatgtct taccgccatc cgtgctcttg tgcaatccgc
     1921 tccctggtcc accggagtta aagctccagg gatagcgccg aaattgccac atcccggcat
     1981 agacaatgct gggctcctgt ggatcaatcg cgacatcgga acatccggta tcggcattga
     2041 catacaacat cttattccac gttttgccgc catccgtggt tttgtacagt ccgcgatctt
     2101 cgttcccatc ccacaaatgc cccaatgcag ccacatacac cacatctgga tttttgggat
     2161 caatgacaat tttcccaatc cgctcggttt tttcgaggcc catcatttgc caggtttcac
     2221 cagcatcagt ggatttatag agtccggttc caaccgaaac gctattgcga acccacgatt
     2281 cccctgtgcc aacccagacg gtatcaggtt tggactgatc aaccgcaatc gcaccaattg
     2341 aaaatgcatg cttgtcaaaa atgggttgaa aggtggttcc tccattcacc gttttccaga
     2401 caccgccgct cgcagtgccg acatagagaa tcaaagggtt ggagttcaca ccatcaatgt
     2461 aggaaatgcg accactcatc gtggccggtc cgatagagcg agcttcgacg gcaccaaaac
     2521 tgccggaatc aagcttgatg gggtttgatt gctgggctga agccagcaca acctgggtta
     2581 aacacaacac aagaatacac atgaagcgtg caaaagagat acatacctta ggcttgcgca
     2641 cggatgccct cccaaatgaa gtttggatcg ttcgtttttt taatgaatcg gttttggcaa
     2701 ctgactgcag ggggtaatgt ttggcgacag ggagacacaa aattgagaat gaagaattga
     2761 gagtgaagaa ttgagagtga agaatgaaga atgaaaaaac gggttc
//
LOCUS       DAOQDO010000649         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29669_length_2806_cov_2.741549, whole genome shotgun sequence.
ACCESSION   DAOQDO010000649 DAOQDO010000000
VERSION     DAOQDO010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29669_length_2806_cov_2.741549"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="PKZ53_11105"
     CDS             <1..1750
                     /locus_tag="PKZ53_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ41029.1"
                     /translation="SVILTHSTLVETLPAQTAQILCLEQLQLPAYTTPIEQVKQPAAS
                     PVYVIYTSGSTGMPKGVAIKHQSVVNLACALAPKFGLTPADRVLQFASLSFDTAIEEI
                     FPTLQAGATLVCVKDVRMSSAVELLETITATGITVLDLPTAYWHLLVREMVKGKLPVP
                     PSLRLVILGGEQASNEVLDLWFGLVGDRVRLINTYGPTEATVSATLYEARPDQKLTSK
                     VPIGRPIANTQVLVLDQHLKPVPTGVPGELFIGGDGVAIGYLNRKDLTEDRFVIRAQG
                     LGLGAEDRAQGSQSRAEGSGLRAEDRAQGFQSSMISTLQPSAPSPMSSALSPRFYQTG
                     DLVRWLADGNLEFLGRVDDQVKIRGYRVEPGEIEKVLATHPDIAEVIVLAKSDRQGNQ
                     QLIAYVVPRSGPVSVETAHHLEHQIQAFLHDRLPEFMIPSVFVVLERFPLTPNGKIDR
                     TALPQAEAHAETTFVAPRNPVEAELAQLWAEVLGREQVGVHDTFFSLGGHSLLAAQIV
                     FEIRERFQVDIPLRLIFERPTIADLALAILQLQAEKADAAEMETMLAELDQLSDDEVR
                     LLLASESHNGSKDPEE"
     gene            1747..>2806
                     /locus_tag="PKZ53_11110"
     CDS             1747..>2806
                     /locus_tag="PKZ53_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ41030.1"
                     /translation="MSDLRNRIAGLSPEKQALMALRLKQKQSEGTSTQTIPHRTPAER
                     YRLSFAQQRLWFLDQLEPNNPAYNVPIALRLRGPLDAPALERALTELIARHESLRTSF
                     ASGEGDPVQIIHPPQPFHLAVDSIEAEHISDARLVELAAIEARLPFDLASGSLLRARL
                     IRLSDTDHVVLVTIHHIVFDGWSAGIFIRELALLYHAFARGEASPLPKLAIQYADFAE
                     WQRRWLTGETLQTQLDFWKDHLGQEHPLLELPTDFPRPALKRFQGATETIQLSRELLT
                     RLKQLASETDATLFMTLLAAFQTLLFRYTGQTDIRVGTPISGRTSAETKNIIGLFANT
                     LVLRAELSEKPTFRQLLAK"
BASE COUNT          617 a          830 c          730 g          629 t
ORIGIN      
        1 atcggtcatt ttgacccact caacactggt tgaaacctta ccagcacaaa cagcccaaat
       61 cctgtgcctg gagcagttgc aattaccggc ctacaccacg ccaatcgaac aggtcaagca
      121 accggcggcc agtccggtgt atgtcattta cacctcgggt tcgactggaa tgcccaaagg
      181 cgtggccatt aaacaccagt ccgtggtcaa tctggcctgt gcgctggcac cgaaatttgg
      241 cctgacaccc gctgaccggg tgttgcagtt tgcctcgctc agctttgaca cggctatcga
      301 agaaattttc ccaacgctcc aggctggcgc cacgctggtt tgtgtcaaag acgtgcggat
      361 gtcttccgcc gtggaactcc ttgaaacaat taccgcaacg ggcattacgg tgcttgattt
      421 gccaaccgct tactggcatt tgctggttcg ggaaatggtc aaaggcaagc ttccagtgcc
      481 accatcgctc cggctggtga ttttgggtgg cgagcaggcg tccaacgaag tcctcgacct
      541 ctggtttgga ctggttggcg accgggtacg gctcatcaac acctatggtc caaccgaggc
      601 cacggtgtcc gccacgctct atgaagcccg cccagaccaa aaactgactt cgaaagtccc
      661 tatcggtcgt ccgattgcca atacccaggt gctggtgctc gatcaacacc tcaaacccgt
      721 cccgactggc gtccccggtg aacttttcat cggcggcgac ggagtggcca tcggctacct
      781 gaatcgaaag gatttaacgg aagatcggtt cgtcatcagg gctcagggtt tagggctggg
      841 ggctgaagac agggctcagg gctctcaatc cagggctgag ggctcagggc tgagggctga
      901 agatagggct cagggttttc aatcatcgat gatttctacc cttcagccct cagccccaag
      961 cccgatgtct tcagccctca gccctcggtt ttaccaaacc ggcgaccttg tccgctggct
     1021 ggcggatggg aatctggagt ttttgggacg ggtggacgat caggtcaaaa ttcgtggcta
     1081 tcgggtcgaa cccggtgaaa ttgaaaaggt actggcgacg catcccgaca ttgccgaggt
     1141 aattgtcctg gccaaatctg accggcaggg caatcagcaa ttgattgcct atgttgtgcc
     1201 acgttccggg ccagtttctg tcgagaccgc acaccatctg gaacatcaga ttcaggcgtt
     1261 tttgcacgac cgattgccag aatttatgat tccgtcggtg tttgtcgtcc tcgaacgctt
     1321 cccgctgacc ccaaatggca aaattgaccg caccgcgctg cctcaggccg aagcccacgc
     1381 cgaaacaacc tttgttgccc cgcgcaaccc ggttgaagcc gaactggccc aactgtgggc
     1441 cgaagtgctt ggccgcgaac aggtcggcgt tcacgataca tttttctcac ttggtggaca
     1501 ttccttactt gctgcccaaa tcgtgtttga aatccgggag cgttttcagg tagatatacc
     1561 gcttcgtttg atcttcgaac gccccacaat cgccgatttg gccctggcca tccttcaact
     1621 tcaagccgag aaagccgacg ccgccgaaat ggaaaccatg ctggcggagc tggatcaact
     1681 ttccgatgac gaagtccgat tgctcctggc cagtgaatcg cacaatggta gcaaggaccc
     1741 cgaagaatga gtgatttacg caatcggatc gccgggctgt cgcctgaaaa acaggccctg
     1801 atggcgctcc gcttaaaaca aaagcaatcc gaaggaactt ccacccaaac cattccccat
     1861 cgaaccccag ccgaaaggta tcgcctgtcg tttgcgcaac agcgcctgtg gtttctcgat
     1921 caacttgagc caaacaaccc ggcatataac gttccgattg cgctcagact ccgtggtcca
     1981 cttgatgctc cggccctgga acgggcactc actgaactga ttgcccgtca cgaaagcctg
     2041 cggacgtcct ttgcctctgg cgaaggcgac ccggtccaga tcattcatcc accccagccc
     2101 tttcacctcg cggttgactc aatcgaagcc gaacacatct ccgacgccag actggtcgaa
     2161 ctggccgcca tcgaggcccg cctcccattt gatctcgcca gcggatcgtt gctccgggcc
     2221 agattgattc ggttaagtga caccgatcat gtggtactgg tcaccattca ccacattgta
     2281 tttgacggct ggtccgccgg aatttttatc cgtgaactgg ccctgctcta tcatgccttt
     2341 gcgcgtggcg aagcctcgcc actgccgaaa ctggcgattc agtatgcgga ttttgccgag
     2401 tggcagcgta gatggctgac cggtgaaaca ctgcaaacgc aactcgattt ctggaaggat
     2461 catttgggtc aggagcatcc gctgctcgaa cttccaaccg atttcccacg cccagctctc
     2521 aagcggtttc agggtgcaac cgaaaccatc cagctttcgc gtgagctgct gacccggtta
     2581 aaacaactcg cttcagagac ggatgccacg ctgtttatga cgctgctggc agcgtttcaa
     2641 accttacttt tccggtatac ggggcagact gatattcgtg ttggaacgcc aatttctgga
     2701 agaacttcag ccgaaaccaa aaacatcatc gggctctttg ccaacacgct ggtattgcgg
     2761 gctgaattgt cggaaaaacc aacgtttcgc cagttgctgg ccaaag
//
LOCUS       DAOQDO010000650         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29675_length_2805_cov_6.156000, whole genome shotgun sequence.
ACCESSION   DAOQDO010000650 DAOQDO010000000
VERSION     DAOQDO010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29675_length_2805_cov_6.156000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKZ53_11115"
     CDS             <1..488
                     /locus_tag="PKZ53_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ41031.1"
                     /translation="AARQQALQVDPTQPVHSIVVMDQLLAKWNIPDQFAMVLMSLLAV
                     LALVMAATGLYAMLTFLVSSRTREIGVRIALGAHSRDIFRLIFHESSKLLVAGVVIGL
                     AVASVCARLVMSLLFQLSPADPITLGMVVVFLMALGLFAAYWPARRAVKIDPLIALRN
                     E"
     gene            691..2055
                     /locus_tag="PKZ53_11120"
     CDS             691..2055
                     /locus_tag="PKZ53_11120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNJ41032.1"
                     /translation="MTQTRSSVVVCGAGMAGVAAAYHLVVNHGIRDVLLLDERPPLTL
                     TSDKGTGAYRNWWPGPGHQMVQFINRSIDLLEELAQLSNNVFELNRRGYVFLTGDPEQ
                     AQTLRETASAISALGAGPVRDHSDLSTYVPSPAEGFEHLPAGADFLTSPDAIQHLFPY
                     LTSEAQAMLHVRRAGWMIPVRLGQWMLDQITAQGGRFRQGKVSRILTSGNRVQGVEVD
                     GKDTVASDRVVIAAGPLLNRVAALAGLELPVTCELHGKIALNDTRGVLPDDAPLLIWN
                     DPVMLPWTDAECDAIRARGETHLLERFPAGVHVRPRGKSPNHELLIIWTYDTHTQEAT
                     FPPIFPNEYPEVLIRGLSRLIPGLTAYFGQGATAYTDGGYYCKTSENRPLIGPLPVEG
                     MYVIGALSGFGIMASQAAAELLAAHVTGTSLPDYAPVFRLERYADPAYQALLETGSAW
                     SGQL"
     gene            2329..2757
                     /gene="bamD"
                     /locus_tag="PKZ53_11125"
     CDS             2329..2757
                     /gene="bamD"
                     /locus_tag="PKZ53_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNJ41033.1"
                     /translation="MKIFQLALILVALTASLLIGGFTSSAFAQSNAQNPLEEYGTRDP
                     ELEMKASKNVEVAKYYLGQKKYKQSISRLVEIVEIYPQYSRFDQVLFYLAESYLANGE
                     LGEAKNRYGELIEKFPESELFKKAEKQLKKVEEKQKQAKG"
BASE COUNT          670 a          700 c          724 g          711 t
ORIGIN      
        1 aggcagcccg tcaacaggcg cttcaagttg acccaaccca accggttcat agcatcgtgg
       61 tgatggatca gttactggct aagtggaata ttccagatca atttgcgatg gttttgatgt
      121 cgctgctggc ggtgctcgca ctggtgatgg cggccacggg gttatatgcc atgctgacat
      181 tcctggttag ttcccgaaca cgtgaaattg gtgtccgcat tgcccttggt gctcacagcc
      241 gcgatatttt ccgcttaatt ttccacgagt catccaaact gctggtggct ggtgtcgtga
      301 ttggtctggc tgtcgcgagt gtgtgtgctc gattggtgat gtcactgtta tttcaactca
      361 gtccagcaga tccgatcacc cttggaatgg tggtggtctt tctgatggca ttgggactct
      421 ttgccgccta ctggccagcc cgaagagccg tcaaaattga cccactgatt gcgcttcgaa
      481 atgagtgaca ccatttaggg ttcagggttc agggttcaga gaaatacagt cagtgaagaa
      541 acagttaaac aactcaatta actaaactta gcggactact gattattaaa tactgactac
      601 tgactggcag aaaactcggc agaaaaaaga aagtgcgcta cacttggagc ttcctcaact
      661 actctcaatt tctccaggtg agcttttctc atgacacaaa cacgcagttc agtggtcgtg
      721 tgcggtgccg gtatggccgg agttgccgcc gcctaccatc tggttgtaaa ccacggcatt
      781 cgcgatgtcc tcctcctgga tgaacgccca ccgctgacac ttacaagcga taaaggcact
      841 ggtgcctatc gcaactggtg gcccggcccc ggtcaccaga tggtgcagtt catcaatcgc
      901 agcattgact tgctcgaaga actcgcccag ctcagcaaca atgtgtttga actcaatcgc
      961 cgtgggtatg tcttcctgac cggagatccc gagcaagccc aaaccttgcg ggaaacggcc
     1021 agtgccattt cggcactcgg tgcgggtccg gtgcgcgacc attccgacct ttccacgtat
     1081 gttccgtctc cagccgaagg gtttgaacac cttccagccg gcgcggattt tctgacgagt
     1141 ccagacgcga tccagcatct ttttccctat ctcaccagcg aagcacaggc catgctccac
     1201 gttcgacggg ctggatggat gattccagtc cggttgggcc agtggatgct cgaccagatt
     1261 acggcgcagg gtgggcgatt cagacagggg aaggtcagtc ggattcttac atcgggaaac
     1321 cgcgttcaag gcgtcgaagt ggatgggaaa gatacagttg ccagtgatcg ggtggtgatt
     1381 gcggcaggcc cattgttaaa tcgagtggcg gcgcttgctg gcctggaact tccggttacg
     1441 tgtgagctgc acgggaaaat tgcactcaac gacacccgtg gcgtgcttcc ggacgatgcc
     1501 ccgcttttga tttggaatga cccggtgatg ctgccctgga ccgatgccga atgcgatgca
     1561 attcgggcac gcggcgaaac acatttgctg gaacgcttcc ccgcgggtgt gcatgtgaga
     1621 ccacgtggaa aaagcccaaa ccatgagctt ttaatcatct ggacctatga cacccacacc
     1681 caggaagcaa ccttcccacc cattttccca aacgaatatc cggaagtcct gattcgcgga
     1741 ctttcacgtc tcatacctgg actcacagct tattttggcc agggcgccac cgcctatacg
     1801 gatggcggat attattgtaa aaccagtgaa aatcgcccgt tgatcggccc gcttccggtc
     1861 gagggaatgt atgtcatcgg agcgctctcc gggtttggga ttatggcctc gcaggctgcc
     1921 gccgaactgc tggccgcgca tgtgacgggc acttccctac ctgactacgc accggttttt
     1981 cgtctggaac gctatgctga cccggcctat caagcactgc tggaaacggg cagtgcctgg
     2041 tcaggacagc tttgagaatt gaggatgaag aactgagaat gaagaattga gaatgaagaa
     2101 tgaagaatta gaggaaacct ggaactattt cccggttggt acattgtttc aatgaatttt
     2161 taaatagctt aaaaacacag gcttaacgaa tcacagagtt gcctgatttc tttattctca
     2221 attcttcatt cttcattctt cattcttcat tcttcattct tcattcttca ttcttcattc
     2281 tcagttcttc attctcagtt cttcattcat ctttagggag tttttgttgt gaagattttt
     2341 caactcgcac ttattttggt cgcactcacg gcaagccttt tgattggtgg ctttacatcg
     2401 agtgcctttg cacaatccaa tgcgcaaaat ccgcttgagg aatatggaac gcgcgatccg
     2461 gaactggaaa tgaaggcttc gaagaatgtt gaagtcgcca aatactatct ggggcagaaa
     2521 aaatacaagc agtccatcag ccggctggtc gagattgttg agatctatcc gcagtattca
     2581 cggtttgatc aggtgttgtt ttatctggct gaatcgtacc tggccaatgg ggaattgggt
     2641 gaagccaaaa accgctatgg cgaattgatt gaaaaattcc ccgaaagcga actcttcaaa
     2701 aaagctgaaa aacaactcaa aaaagttgag gaaaagcaga aacaggcaaa gggatgactg
     2761 atgacaggaa tgagagggtg actctgtcat tcctgtcatc agttg
//
LOCUS       DAOQDO010000651         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29686_length_2805_cov_2.874182, whole genome shotgun sequence.
ACCESSION   DAOQDO010000651 DAOQDO010000000
VERSION     DAOQDO010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29686_length_2805_cov_2.874182"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="PKZ53_11130"
     CDS             <1..1941
                     /locus_tag="PKZ53_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41034.1"
                     /translation="KEFDLVLLDILMPEMDGFQVLKRLKADQRMRYIPVIMISALTEI
                     ESVARCIEMGAEDYLPKPFNSMLMRARIDACLEKKRLHDQELQYLQLVSKLTNAAAAV
                     ESGTFHPSQLEEVTAHPGELGRLARVFTQMAREIAARETMLEAKIQERTLELARLVER
                     LNLTVDQLQLSERKALEASRAKSVFLANMSHELRTPLNAIIGFVQMLQRKEPRDADDR
                     EHLGIIRRSSEHLLGIINDVLSISKIEAGKVTLTETVFKLPRLLKTLEDMFRIRAQAK
                     RLVINFELSKDLPRYVYGDEGKLRQILINLLNNAVKFTAYGGITLRAQWLADKLQCEV
                     TDTGLGMSPDEIQTLFEPFTQTESGVKSAEGTGLGLTITRNYINLMNGNIRIQSSSGR
                     GTTVTFEVTLVPASKDKFYQEARRVVCLGDNQMVPKMLVVDDQNDSRLLLKEILTPIG
                     FEVREARNGLEAVEAYKTWHPDVIWMDVRMPESDGYEATRAIRCLELTNSSSSLPRTA
                     IIAISAGVFDTDREAILAAGCDAFLAKPFHEIELFELLEQYLGLEFIYEEDVSYSPDL
                     FGIINSDPMPIQAESPMLPDTWIDRMNNALDEGDIERAMSLLELIKSENEQLVARLKL
                     LLSSYKVEEIQDFLKEIKQQKG"
     gene            2058..2753
                     /locus_tag="PKZ53_11135"
     CDS             2058..2753
                     /locus_tag="PKZ53_11135"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ41035.1"
                     /translation="MSHTEFYFAKIAAAFVRGTRSGPILIPDQPEELWTAPLDVLLDS
                     ELEAIVQCGLDQGLRLHRFKRTMGLARVQRVLGLLKGMSPATLLDIGSGRGAFLWPLI
                     EEFPEMPVTAIDCDPKRVADIQAVATGGFSQLAAHEMDVTSLEFPDNTFDVVTALEVL
                     EHIPAFDQAIHECCRVAQRFVIVSVPSHEDDNPEHIHLFDQTRLTEALWAAGAHRVSC
                     EYVLNHLIVVAGV"
BASE COUNT          675 a          677 c          740 g          713 t
ORIGIN      
        1 aaagagtttg acctggtctt gcttgatatt ttaatgcctg aaatggatgg gtttcaggtt
       61 ctgaaacggt taaaagccga tcagcgaatg cgctacattc cggtcatcat gatttcagcg
      121 ttaaccgaaa tcgaaagcgt tgcccggtgt attgaaatgg gggcagaaga ttatctgcca
      181 aagccattta attccatgct gatgcgggcc cgtattgatg cctgccttga gaaaaagcgc
      241 cttcacgatc aggaattgca gtatttgcaa ctggtttcga aactgaccaa tgccgctgct
      301 gcggttgaat ctggcacttt tcatccaagc cagttggagg aagtcaccgc tcatcctggt
      361 gaactcggtc gcctggcccg tgtgtttacc cagatggctc gtgaaattgc ggctcgtgaa
      421 accatgctgg aagccaaaat ccaggagcga accctcgaac tggcgcgact tgtcgaacgc
      481 ctcaacctca cggttgatca gttgcaactc tctgaacgca aagcccttga agccagtcgt
      541 gccaaaagcg tgtttctggc caatatgagc cacgaattgc gaacacccct gaacgccatt
      601 attggatttg tgcagatgct gcagcgtaaa gaaccgcgtg atgctgatga ccgtgagcac
      661 ctgggaatta tccggcgcag cagcgagcat ttgctcggaa tcatcaacga tgtgctttca
      721 atttctaaaa ttgaagctgg gaaagtgacg ctgaccgaaa ctgtctttaa gttgccacgc
      781 ctgctcaaaa cgctggagga catgtttcga attcgcgccc aggccaagcg actggtgatt
      841 aactttgagt tgtcaaagga cttacctcgt tatgtctatg gcgacgaagg aaagctccgt
      901 cagattttaa tcaatttact caacaatgcg gtcaagttta cggcttacgg tggaatcacg
      961 cttcgggcac aatggctggc tgataagttg caatgcgaag tcactgacac cggactcggc
     1021 atgagtcctg acgagatcca gacgttgttt gagccattta cgcagaccga aagtggcgtc
     1081 aagtcagccg aaggcaccgg gttgggactc accatcaccc gaaactatat caacctgatg
     1141 aatgggaaca ttcggattca aagctccagt ggacgtggaa caaccgttac ctttgaagtg
     1201 acgctggtcc cggcttcaaa agataaattt tatcaggaag cgcgccgggt ggtttgcctg
     1261 ggcgataacc agatggttcc aaagatgctg gtcgtggatg atcaaaatga ctcccggttg
     1321 ttattaaaag agattctgac acccattggg tttgaagtcc gggaagccag aaatggcctg
     1381 gaagcggttg aagcctacaa aacctggcat cctgatgtca tttggatgga tgtgcggatg
     1441 ccagagtctg acgggtacga agccacccgc gccattcgct gcctggaatt gaccaattcc
     1501 agtagctcgt tgccccgaac ggcgattatt gccatttcag ccggtgtgtt tgatactgac
     1561 cgggaggcaa ttttggccgc cgggtgcgat gcgtttctgg cgaaaccgtt tcacgaaatt
     1621 gagttgtttg agttacttga gcaatacctt gggctcgaat ttatctatga agaagatgtt
     1681 tcgtattcgc cagatttgtt tggaatcatc aattcagacc ccatgccgat tcaagctgaa
     1741 tctccgatgc tgccagatac ctggattgac cggatgaaca atgcccttga tgaaggagac
     1801 attgaacgag ccatgagttt gctcgaactc atcaaatctg aaaacgaaca gcttgtcgcc
     1861 cgtttgaaat tattgctcag cagctataaa gtcgaagaaa ttcaggactt tctcaaggaa
     1921 atcaaacagc aaaaggggtg atccaaatcc gggttccggg tttttggaag ggatgaagga
     1981 tgaggatgaa atacaaccta ttcttcagcc cgaattcttc agtccgaatt cttcagcccg
     2041 ctttcttctg ccccaccatg agtcacaccg agttttattt tgccaaaatc gcggcggcct
     2101 ttgttcgtgg gacacgctca gggccaatcc ttattcctga tcagcctgaa gaactctgga
     2161 ccgcgccgct tgatgtattg cttgactcag aacttgaagc aatcgtgcag tgtggtctgg
     2221 accaggggtt gagactccat cgctttaagc gaaccatggg gctggcccgt gttcagcgtg
     2281 ttttgggtct tttaaaagga atgtcaccag cgacgttgct tgatattgga agtggtcgtg
     2341 gcgcgttttt gtggcccctc atcgaagaat ttccagaaat gccagtcacc gccattgatt
     2401 gtgaccccaa acgggtggct gatatccagg cggtggcaac gggtggcttt tcccagcttg
     2461 ccgcacacga aatggatgtc acctccctgg agtttccaga taataccttt gatgtggtca
     2521 cggcccttga agtgctggaa cacattccgg cctttgacca ggccatccac gaatgttgtc
     2581 gtgtcgcaca gcggtttgta attgtgagcg tcccttccca cgaagatgac aacccggagc
     2641 acatccattt atttgaccaa acccgactca ctgaagcact ttgggccgct ggtgcccatc
     2701 gtgtttcgtg cgaatatgta ctgaaccatc tgattgtcgt ggcgggggtg tgagttccgg
     2761 gttccgggtt ccgggttccg ggttccgggt tccgggttcc gggtt
//
LOCUS       DAOQDO010000652         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29723_length_2803_cov_2.973799, whole genome shotgun sequence.
ACCESSION   DAOQDO010000652 DAOQDO010000000
VERSION     DAOQDO010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29723_length_2803_cov_2.973799"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKZ53_11140"
     CDS             complement(<1..825)
                     /locus_tag="PKZ53_11140"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNJ41036.1"
                     /translation="MSFQSDGLGAASNEVQTERDLTAPQVGMADSHPEALNGIDLEYQ
                     KLFQLFGCDIDGTISRDDLFAQLQGCGIWVNDPRLLKTVETLQALEANHRITFSQFKE
                     ICHQNSSLIKKAIQGNLIIPEFQEMVAVFQEMFTRVRKNTSGKVANYIPQLSRVNPEQ
                     FAVSICTVDGQRLSLGDAQVDFCLQSVSKPVNYCLALEEHGETHVHRHVGREPSGRGF
                     NELTLNNDGLPHNPLINSGAIMCCSMIKPGLEPADRFDYVLNTWGRLTGGQRVGFNN"
     gene            1138..1992
                     /locus_tag="PKZ53_11145"
     CDS             1138..1992
                     /locus_tag="PKZ53_11145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41037.1"
                     /translation="MTQSDYTIKTMTRQELDIAVAWAAAEGWNPGRHDVDSFYAADPS
                     GFLIGYLGNEPIASISAVKYGDSFGFIGFYIVRPEYRGRGYGIQIWNAAMAYLSGRTI
                     GLDGVLAQQENYQKSGFTLAYRNIRYQGTGGGVRPTDSSLVPLSTIPFDEVCAYDRPF
                     FHDNRTSFLKRWIAQPESIALGVLGADANLAGYGVIRPCQSGFKIGPLFADSPQLAED
                     LFLALKAGAPGEAPVFLDIPEVNPAAQELVQRHTMVSMFETARMYTQTCAALPINRLF
                     GVTSFELG"
     gene            2363..>2803
                     /locus_tag="PKZ53_11150"
     CDS             2363..>2803
                     /locus_tag="PKZ53_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41038.1"
                     /translation="MFLSIFLWIHHQVDSDLTRTRKLISNQRLIAFHHKPLGPHEHSN
                     IRTIFTDSHVTDVTRFQNTLVAATTGGLVQIGNPARHFTLRDGLPEHNLTSLAVFHDT
                     LWIGTNEHGLITFDGVQFQHYTWPNHNQRITALLADAHSLLIGTQ"
BASE COUNT          682 a          740 c          680 g          701 t
ORIGIN      
        1 attgttaaag ccgacgcgct ggcctccggt cagccgtccc caggtgttga gcacataatc
       61 aaaccggtca gccggttcaa gtcccggctt gatcatcgaa cagcacataa tcgcaccgga
      121 attgatcaat ggattatgcg ggaggccgtc gttgttgagc gtcagttcgt taaacccgcg
      181 cccgcttggt tcgcgcccaa cgtgccggtg gacgtgcgtt tcgccgtgct cttcgagcgc
      241 caggcagtaa ttaaccggtt tgctgactga ttggaggcaa aaatccacct gagcatcgcc
      301 cagcgacaat cgctgaccat caacggtgca gattgagacc gcaaattgtt cgggattaac
      361 ccggctgagt tgtggaatgt aattggcaac ttttcccgaa gtgttcttcc gaacgcgggt
      421 gaacatctcc tgaaagaccg ccaccatttc ctgaaactcg ggaatgatca aattcccctg
      481 aatcgctttt ttgatcagac tgctgttctg gtggcaaatt tctttgaact gggaaaaggt
      541 aatccggtga ttggcttcga gtgcctgcag cgtttcgact gttttcagaa ggcgcggatc
      601 attgacccaa atgccacagc cctgaagctg tgcaaacaga tcatcacgcg aaatcgtgcc
      661 gtcaatatcg catccgaaga gctggaacag tttttgatat tcgagatcaa tcccattgag
      721 tgcctcaggg tgactgtccg ccattccgac ttgtggagca gtgaggtctc gttctgtctg
      781 gacttcattt gaagccgccc ccaggccgtc tgattgaaac gacatagcaa gtcctttcag
      841 aagttcaggt ttgaaatggt tcagatggac gaaaaagagt ggatgtcaag tcagtaccac
      901 ctgcgtgagc gggtgggttg ccgcatggac ttcattcaat tcccggtaaa tgcttaaccc
      961 tgatactgtc tcatcaactg gataggcaaa tcttgtcttg cttgagatcc ttttgcaagt
     1021 aagagttgtg ctgaccggaa ctggcgacgc gcttgccaga actcaggttt gtccttacat
     1081 tgcacaattc aaccaaaatc ctgaaaacca actcaccaaa ggcagtgaaa acgacgtatg
     1141 acccaatcgg actacacgat aaaaaccatg acccgtcagg aactcgatat tgcggtggcc
     1201 tgggcagcgg cagaaggctg gaatccaggg cgccacgatg tagacagttt ttatgcggca
     1261 gacccgagcg gattcctgat cggatacctt ggaaacgaac ccatcgccag catttcagcc
     1321 gtgaaatatg gcgattcttt cggcttcatt gggttttata ttgtcaggcc cgagtatcgc
     1381 ggtcgagggt atgggattca gatttggaat gcggccatgg cctatctgag tggtcgcacc
     1441 atcggacttg atggggttct ggcccagcag gaaaactacc agaaatcagg atttaccctg
     1501 gcttatcgca acatccgcta tcaggggacc ggcggtggcg tgcgtccaac cgattcaagc
     1561 cttgttccgc tgtcaacgat tccatttgat gaagtctgcg cttatgaccg accatttttc
     1621 cacgacaatc gcacgtcgtt cctgaaaaga tggattgccc aacccgaaag catcgcgctt
     1681 ggggttctgg gagctgacgc aaaccttgcg ggatacggtg tcattcgccc ctgtcaatct
     1741 gggttcaaga ttggcccatt gtttgccgat agtccgcaac tggcggaaga cctgttcctg
     1801 gcattgaaag ctggtgcccc aggtgaagcg cccgtctttc ttgatattcc ggaagtcaat
     1861 cctgccgcgc aggaactggt ccagcgacac accatggtca gcatgtttga aacagcccgg
     1921 atgtacactc aaacctgtgc cgcattaccc atcaaccggc tgtttggtgt gacgtcgttt
     1981 gaacttgggt aggacctgta taagccattt ccaaccacgg aaaacacgga aaacacggaa
     2041 aaaaatcaaa cgcctatcaa aaccggcttc tcagattttt tcaattcgac ccgttcatcc
     2101 tctttccaca ctccccatat gtggttgatc tacctggagt tggtgtactt tccaacgctc
     2161 tggctggggc ttgccacaca gtttgatttg gaacccggga ccataatgga cacctctgtt
     2221 gccaattcca atcaaaactg gtcaaaccac aatgagtttt caacttcggc ccaggcgatt
     2281 ttctttccag tggagcaccg tcaaaacccg gcttcacaca cgttcgaaac ttattacatt
     2341 tgtgttttca gcaattgtaa tgatgttcct ttcaatcttt ctctggattc atcatcaggt
     2401 tgattccgac ctgacccgta ccagaaaact catttcaaat caaagactta tcgcctttca
     2461 ccacaaacct cttgggcctc acgaacattc aaacattcga acaattttta ctgactcaca
     2521 cgtgaccgat gtcaccaggt ttcaaaatac actggtggcg gcgacaacgg gcgggctggt
     2581 tcaaatcgga aatccagccc ggcatttcac cctgcgagat ggcctgccag agcacaatct
     2641 gaccagtctg gcggtttttc acgacacatt gtggattggc acaaatgaac acgggctcat
     2701 tacttttgat ggggtacagt ttcaacacta tacctggccc aatcacaatc aacgcattac
     2761 ggccctgctg gctgacgctc attccctgtt gattgggaca cag
//
LOCUS       DAOQDO010000653         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29805_length_2799_cov_2.899052, whole genome shotgun sequence.
ACCESSION   DAOQDO010000653 DAOQDO010000000
VERSION     DAOQDO010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29805_length_2799_cov_2.899052"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKZ53_11155"
     CDS             complement(<1..805)
                     /locus_tag="PKZ53_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ41039.1"
                     /translation="MGVLFGKSKVLGYQIGVVLIWLIWFGLISTAQAQVLPLPSDATT
                     PRNPKPTPATQEDPSELLIRSDLVTLLVTVETTEKKSVPSLQRDEFELLEDGVPQKIE
                     SLVQEVRTPLRMVVLLDISSSVRNRLKFEQEATLRFFREVLRPGDQIAFYAFNHDVVM
                     RQDMTGDLKQLEAATQGLKAKGGTAVYDAVYQAAQKLGRENGRRIIILVSDGSNTISR
                     VSQAKAIDEVRRSEAVLYGIYSAAQTSYDNFMTPRGRFTLQALCNETGGR"
     gene            complement(825..2177)
                     /gene="trmFO"
                     /locus_tag="PKZ53_11160"
     CDS             complement(825..2177)
                     /gene="trmFO"
                     /locus_tag="PKZ53_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNJ41040.1"
                     /translation="MVLTQVSIIGGGLAGSEAAWQVAQRGINVTLYEMRPVQPTPVHT
                     TSNLAEIVCSNSFKSDESNSAPHLLKEELRRAKSLLIEIAEQVRVPAGAALAIDREKF
                     SAEVTRRLSDHPHITIKREELTRIPADGPVIIATGPLTSPALVSEITRLTGDEQLYFF
                     DAISPVVDAETIDYSVVFRAARYGKGGDDYLNCPMSKDQYEVFVDALLAAEKVPHKAF
                     EADSAKYFEACLPIDVAVTRGRETLRFGPLKPVGLIDPRTGEMPHAVVQLRQENLLAD
                     SYNLVGFQNSIKWGPQKQVLQLIPGLERAEFLKFGQVHRNTYINAPNILTNTLQVRHE
                     PRIFFAGQISGVEGYVESLATGLVAGRYAADLVQHRSPVPVPRASAIGSLIHYISHCE
                     PKHFQPVNITFALLPPLDEAERKKLKRKPDRHARQIAIGLAAFEEWLNKQTQAKAVAE
                     "
     gene            complement(2334..>2799)
                     /locus_tag="PKZ53_11165"
     CDS             complement(2334..>2799)
                     /locus_tag="PKZ53_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ41041.1"
                     /translation="ELQKVAIEMRAQTNANQSAEAAAQTRVELSRVEASLDTAIKRYE
                     SRAQQNNLVVRNETTVLGIVTTDLTRQLAAFFGVAQTGVLVNSIDARGFLSQIGIQAG
                     DIIIELNGQPVISHNQLNWMLNNQPLVLESISIVRKKQTLKFALNRLHQPSR"
BASE COUNT          683 a          717 c          705 g          694 t
ORIGIN      
        1 cccgcccccc agtttcatta cacaatgcct gtaatgtaaa ccgcccccgt ggcgtcataa
       61 agttgtcata tgaggtctgg gccgccgagt aaatgccata taaaacagcc tcggaccgcc
      121 gaacttcatc aatggctttg gcctgtgaga cccgactgat ggtgtttgat ccgtctgaga
      181 ccagaatgat aatccggcga ccgttttctc gaccgagttt ttgagcagcc tgatagaccg
      241 cgtcatacac cgcagtccca cctttggctt tgagcccctg ggtcgccgct tcaagttgtt
      301 tcagatcccc agtcatatcc tgccgcatca caacatcgtg gttaaacgcg taaaaggcaa
      361 tctgatcccc agggcgaagc acctcgcgga aaaaacgaag ggtggcttcc tgctcaaact
      421 tcaggcgatt gcgcacactt gaactgatat caagcaaaac aaccattcga agcggagtcc
      481 tgacttcttg aaccagagac tcgatttttt gagggacccc atcctccagc aactcaaact
      541 catcccgttg gagagaaggt acggattttt tctccgttgt ctcaaccgtg accagcagcg
      601 tgaccagatc agatcgaatg aggagttcac tcggatcctc ctgggttgcg ggagtgggtt
      661 tggggtttcg gggagttgtg gcatctgaag gaagtggcaa cacctgtgcc tgcgccgtac
      721 tgatcaggcc aaaccatatc aaccagatca atacaacccc aatctgatat ccaagcacct
      781 ttgattttcc aaacagtaca cccatgccgt aatctctcct ggtctcattc agcgacggcc
      841 ttcgcttgtg tttgtttatt gagccactcc tcaaaggcag ccaacccaat tgcaatttgt
      901 cgggcgtgac ggtcaggctt gcgtttcaac ttcttccgct ctgcttcgtc aagtgggggc
      961 aacaaagcaa atgtaatatt gacgggttgg aagtgttttg gctcacagtg ggagatatag
     1021 tggatcaggc ttccaatcgc tgatgcccgt ggaactggaa ctggtgatcg gtgttgaacc
     1081 agatctgccg cataccgacc agcaaccaga cctgtggcca gggattcaac atagccttca
     1141 acgccagaga tttgaccggc aaagaaaatt cggggctcat gcctgacctg caaggtattg
     1201 gtcaaaatat tgggcgcatt gatataggta ttgcgatgga cctgaccaaa tttaaggaat
     1261 tcagcgcgtt caaggcctgg aatgagttgc aatacctgtt tttgggggcc ccatttaatc
     1321 gaattctgaa atccaaccag gttatagctg tcagccagaa ggttttcctg gcgcaattgc
     1381 accaccgcat ggggcatttc cccagtacgg ggatcaatca atccaactgg cttcagcggc
     1441 ccaaaccgca gcgtctcgcg cccacgggtg acagccacat caattggcaa acaggcttca
     1501 aagtatttgg cagaatcggc ttcaaaggct ttatggggaa ctttttccgc tgccagcaag
     1561 gcatccacaa agacttcata ctgatctttg gacattgggc aattgagata atcatcaccg
     1621 cctttgccat accgggccgc tcgaaacaca actgaataat caatggtttc ggcatcaacc
     1681 actggggaaa ttgcgtcgaa aaagtacaac tgctcatcgc ctgtcaaccg ggtaatttca
     1741 ctgaccaggg caggtgaagt cagggggccg gtggcaataa tcactggtcc atcggctggg
     1801 atccgggtta gttcctcacg tttgatggtg atgtgtgggt gatcagacaa ccggcgggtg
     1861 acctcggctg aaaatttttc gcgatcaatg gccagcgctg cacccgctgg cacccgaacc
     1921 tgttcagcaa tttcaatcag taacgatttg gctcgacgca gttcttcttt gaggaggtga
     1981 ggtgctgaat ttgattcgtc tgacttgaag gaattgctgc agacaatctc cgccagattg
     2041 gaagttgtat gcactggggt cggctgaacg ggacgcattt cataaagcgt gacattgatg
     2101 ccgcgctgag caacctgcca ggcagcttca cttccagcca aaccgccacc gatgatggaa
     2161 acctgagtta aaaccataga cgagaaatcc gttcttgagg tcagagttgg gaaaacctgg
     2221 attgtaggtg agcggtcttc tgacaagcaa gaaagtccgg aggaacttct gtgtgatgaa
     2281 gttcctttcc ggacttttac ccactgccga ttaccagttt gcactggtca acatcacctg
     2341 gaaggttgat gcagccgatt cagagcaaac ttgagcgttt gttttttacg cacaatcgaa
     2401 attgattcaa gcaccagtgg ctgattgttg agcatccagt ttaattgatt gtggctaatc
     2461 accggctgac cattgagttc aatgataata tctccggcct gaatgccaat ctggctcaaa
     2521 aaccctctgg catcaatact attgaccagc acaccggttt gggcaacccc aaaaaaggca
     2581 gccagttgcc gggttagatc ggttgtgaca atccccagta cggtggtttc gtttcgaacg
     2641 accaggttgt tttgctgtgc tcgtgattca tagcgtttaa tcgcggtgtc gagtgaggcc
     2701 tcaactctgg acaattcaac ccgggtctgg gccgcggctt ctgccgactg attggcattc
     2761 gtctgggctc gcatttcaat tgccactttt tgcaattca
//
LOCUS       DAOQDO010000654         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29806_length_2799_cov_2.848032, whole genome shotgun sequence.
ACCESSION   DAOQDO010000654 DAOQDO010000000
VERSION     DAOQDO010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29806_length_2799_cov_2.848032"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..993
                     /gene="grpE"
                     /locus_tag="PKZ53_11170"
     CDS             241..993
                     /gene="grpE"
                     /locus_tag="PKZ53_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNJ41042.1"
                     /translation="MSASDLDQDLDHSDDELEDEEAEETLEDLIANLAQELRRVGREV
                     FKVNRAVDRNQELFEDALKELRRINRDATKAASSNDSGAIAQAIFETKASLCKELLGV
                     VDGLELSLTSAKEIVEQLETALDENQTLAEEVAVNFETLSHSLPPEVLAWFSLARTFR
                     WDVTDTINSFHQWLEGQNLIIRRFLTVLAAVGVVPIDSVGNRFTPDRHKAVSVEIRTD
                     VPVGTIIDEEVRGYTLDGKILRFAEVIVTRYE"
     gene            986..2716
                     /locus_tag="PKZ53_11175"
     CDS             986..2716
                     /locus_tag="PKZ53_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNJ41043.1"
                     /translation="MSKTVGIDLGTTNSLVAILENGVPRILERNGERLIPSVVGLSDA
                     GEIMVGQAALNQYVLAPERTIRSIKRKMGSTEKVQLGDKSYLPEEISAFILTYLKKMA
                     EELLGEPVTEAVITVPAYFNDLQRQATKRAGELAGLNVIRIINEPTAAALAYGIDRQE
                     DQYLLVYDLGGGTFDVSVIEQQGEILEVRASNGNVNLGGDDFDEKLLEHLLLYLANDH
                     RYDFKADRKAMARLSRAAERAKVALSNAPYAQATEEFLARYDGNIAHLNLEVNRDEFE
                     DLIADQLDSTCDLIDKALTDAELKAEQLKRVLLVGGSSRIPLVRELLEEHLGLEPSME
                     VNPDEAVALGAAVQAGIIKGDPIAAMLIDVAPHSLGIEVAELFMTNMVPGRFAPLIHR
                     NTTIPTTKSERFFTITPAQDTVEVKVFQGEKMLCAENTSLGSFQFTDIPPSEDPERPR
                     EIIVQFSYNLDGILEVSAFDRHGKRKESMVLNTLATQRAASEPEQTTHFEPELERTIK
                     DALNDATRMETQLETDGKKKAAEKIRTVRHKLEKAHAEGKEPQARKALEKLEDMLYEY
                     EYSLGMKNAE"
BASE COUNT          732 a          737 c          703 g          627 t
ORIGIN      
        1 ccgggaccgt cattttgaat tccaacagcc agattgtcgg ctttgtggtc tgtgaccagg
       61 cgatcttgaa cagcaattcg caactcaaac gcattggcca atgaccaaaa gcgcgataca
      121 ggaccgctac cactccgcta tccgacctca gcctatacaa cctcgaaaga aaatgataag
      181 gtggcgcgtt tcaattgact tcccgaattt caagctacat ccacctgaaa gagtctgctt
      241 atgtctgctt cggatcttga ccaagatctt gaccattccg acgacgaact tgaggacgaa
      301 gaggcagagg aaaccctcga agacctgatt gccaatctgg cccaggagtt acgccgggtt
      361 ggccgcgaag tgtttaaagt caaccgggcg gttgaccgca atcaggaact ttttgaagat
      421 gccctcaaag aattgcgccg aatcaaccgt gacgcgacca aagctgccag ttcaaatgat
      481 tccggtgcga tagcacaagc catttttgaa accaaagctt cgctgtgcaa agagttgctc
      541 ggtgtggttg atggccttga actcagcctg acttcggcca aagaaattgt tgaacagctt
      601 gaaaccgctc tggatgaaaa ccagacttta gctgaagaag tggccgtgaa ttttgagaca
      661 ctttcccatt cccttccgcc tgaggtgctt gcctggtttt cactggcccg cacattccgc
      721 tgggatgtca ccgacaccat caattcattc catcaatggc ttgaaggcca gaacctgatc
      781 atccgccgtt ttctgacggt tttagccgcc gtcggtgttg taccaattga ttcagttgga
      841 aatcgcttta cacctgaccg acacaaagcc gtgtcggtcg aaatccgaac tgatgttccg
      901 gttggaacca tcattgatga agaagtgcga gggtacaccc tggacgggaa aattcttcgc
      961 ttcgcagagg taattgtaac ccgttatgag taaaacagtt ggcattgatc ttggcaccac
     1021 caattcactg gtggccattt tggaaaacgg cgttccgcgc atcctggaac gcaacggcga
     1081 acgcctgatt ccgtccgtgg tcggtctttc tgacgccgga gaaatcatgg ttggacaggc
     1141 agcgctcaat cagtatgtcc tcgcaccaga acggaccatt cgctcaatca agcgcaaaat
     1201 gggttcaacc gaaaaagttc aactcggcga caaatcgtac ctgccggaag aaatttcagc
     1261 gtttattttg acctatctca agaaaatggc tgaagaacta ctcggtgagc cggtcaccga
     1321 agcggtgatt acagttccgg cctactttaa cgacctgcaa cgccaggcta ccaaacgcgc
     1381 tggtgagctg gcgggtctca atgtcattcg catcatcaac gaaccgaccg ccgccgcgct
     1441 tgcctatggc attgaccgtc aggaagacca gtacctgctg gtctatgatt tgggtggcgg
     1501 tacgtttgat gtgtcggtga ttgaacaaca gggcgaaatc ctcgaagttc gtgccagcaa
     1561 tggcaatgtc aatctcggtg gcgatgattt cgacgaaaaa ttgctcgaac acctgctgct
     1621 gtaccttgcc aatgaccacc gctatgactt taaagcagat cggaaagcca tggcacggct
     1681 gagtcgggct gccgaacggg ccaaagtcgc cctgtctaat gccccctatg cccaggcaac
     1741 tgaagaattt ctggcccgct atgatggcaa catcgcccac ctcaacctcg aagtcaatcg
     1801 cgatgaattt gaagatctga ttgctgacca gcttgattcg acctgcgatt tgattgacaa
     1861 ggcgctgacc gatgccgaac tcaaagccga gcaactcaag cgagtcttgc tggtgggtgg
     1921 ttcatcgcgg attccgctgg tgcgtgaact gttggaagaa cacctgggcc tggaaccctc
     1981 gatggaagtc aatccggacg aagccgttgc ccttggggct gccgttcagg ccggaattat
     2041 caaaggcgac ccaattgccg ccatgttgat tgacgttgct ccgcacagtc tgggcatcga
     2101 agtcgccgaa ctctttatga ccaacatggt accaggtcgg tttgcaccgt tgattcaccg
     2161 caataccacg attcccacga ccaaatctga gcggtttttc acgattactc ctgcccagga
     2221 cacggttgaa gtgaaggttt ttcagggtga gaaaatgctc tgtgcggaaa acacatcact
     2281 cggaagcttt caatttaccg acattccacc ttcggaagac cctgaacgtc cccgcgaaat
     2341 tattgtccag tttagctata acctggatgg aatccttgaa gtctcagcct ttgaccggca
     2401 tgggaaacgc aaggaatcaa tggtcctcaa cacgcttgcc acccagcggg cagcctctga
     2461 acccgaacaa accacgcact ttgaacctga actggagcgc accatcaaag acgccttgaa
     2521 tgatgctacg cggatggaaa cccagcttga gacggatggg aaaaagaaag cggctgaaaa
     2581 aatccgcacc gtgcgccaca agctggaaaa agcccacgct gagggcaagg aaccgcaggc
     2641 tcgcaaagca ctcgaaaagc ttgaagatat gctttatgag tatgaatact ccttaggaat
     2701 gaagaatgca gaatgaagaa ttgagaatga agaattgaga actgatagtt tttcagaaaa
     2761 agattccccc ctgggaacgc cggcatcctg ccggcaact
//
LOCUS       DAOQDO010000655         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29864_length_2796_cov_2.897483, whole genome shotgun sequence.
ACCESSION   DAOQDO010000655 DAOQDO010000000
VERSION     DAOQDO010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29864_length_2796_cov_2.897483"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PKZ53_11180"
     CDS             complement(<1..1123)
                     /locus_tag="PKZ53_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41044.1"
                     /translation="MAKNQRTHFWRQALFSGLLLLLLTSGMPAQSPASGTSESDVVLK
                     AMQDELARTLGQLQVKDLEKPYFVEYVITDQEIFSTSATFGALVYTNRNRYRTLQTQV
                     RVGDYALDNSGFLSQQDLFSINLPSEVPVDDEYNALRHSLWLGTDAAYKQAVEAFARK
                     QAFLRNRSDEEPLPDFSREDPVRLMVRRQPMQVDQAHWEQQVREWSAVFRQFPDIQES
                     NVSLQATQVHTYVVNSEGTVTRQPQLIVCVEARASTQAADGSRIRHAVPVYAARFDQL
                     PPASEIASRIRTMAEELSRLRTAPVLADPYSGPVLFTGQAACEMFAQVLPPQFSGQRP
                     PLVEQEEYAAALGLAKSELAARLNRPVFPPTVSIVDDPTQ"
     gene            1377..>2796
                     /locus_tag="PKZ53_11185"
     CDS             1377..>2796
                     /locus_tag="PKZ53_11185"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNJ41045.1"
                     /translation="MAFRRVLHWFRRDFRLEDNTALLTACTQAEEIIPVYIFDPGWLS
                     RPDASPERGVFLLDSLHSLQQKLSQVGSYLVVRQGDPAQVLDQLLNETQAEAIFANRI
                     VDPYFRTQGRQLDQRLRQRGCEVRSCQDVMLHPPQSVLTKAGTPYTVFTPYKNNALTV
                     PVQTPQPAPHRITTPAGIASEPIPTIQELGFSTSAILPKGGIEEAHRRLDEFAASRLN
                     QYANQRDLPYLEGTSRLSPYLHFGCVSPRRVFWTAFQALHQTQPATAPRESVETWISE
                     LLWRDFYLQILYHFPHVETGAFKPVYNDLVWDTSEENFARWCQGQTGFPIVDAGMRQL
                     NTTGWMHNRVRMIVASFLTKDLLINWQWGERYFMQHLVDGDLAANNGGWQWAASTGTD
                     AQPYFRIFNPISQGQKFDPKGEYVRRYIPELKHVPEKWIHTPWDIPAKYQDSLRCRVG
                     IDYPKPVVDHARQRMLALDMFQR"
BASE COUNT          673 a          771 c          701 g          651 t
ORIGIN      
        1 cctgggttgg atcatccacg attgaaaccg taggcggaaa cactggacgg ttcaaccggg
       61 cagccagctc acttttggcc aggccaaggg ccgctgcata ctcttcctgc tcgaccagag
      121 gtggacgttg tccggaaaat tgtggcggca acacctgagc aaacatttca caggccgcct
      181 gcccggtaaa cagcacgggc ccggaataag gatccgccag aaccggtgcg gtgcggagcc
      241 ggctgagttc ttcggccatg gtgcgaatgc gactggcaat ttccgaagcc ggcggcaact
      301 ggtcaaaccg ggcggcatat actggcaccg cgtgacgaat gcgggatccg tcagccgcct
      361 gggtgctggc ccgggcttca acacagacaa tcaactgggg ctgacgcgtc acagtgcctt
      421 cgctattcac cacataggta tggacttgtg tcgcctgcag actgacattg gactcctgaa
      481 tatccggaaa ctgacgaaac accgccgacc attcccgcac ctgttgttcc caatgggcct
      541 gatcaacctg cattggctgg cgacgcacca tcaatcggac cggatcttca cgcgaaaaat
      601 caggcagggg ttcttcatcc gagcgattgc gtaaaaaggc ctgcttccgg gcaaaggctt
      661 cgaccgcctg tttgtacgcc gcatcggttc caagccagag gctatgccgc agggcattgt
      721 attcatcatc aaccggaact tccgatggca aattgattga aaagagatcc tgctgactca
      781 ggaacccgct gttatcgaga gcataatccc caacccgtac ctgggtttgc agggttcgat
      841 accggttgcg gttggtatag accagagcgc caaacgtggc agaggtgcta aaaatctcct
      901 ggtcggtaat gacatactcg acaaaatatg gtttttcgag atcttttacc tggagctgcc
      961 caagcgtgcg cgccagttca tcctgcatcg ccttgagcac cacatccgac tcactggtcc
     1021 cagatgcggg tgactgtgcc ggcatacccg aggtcaataa aagcaacagc aatccgctga
     1081 acagtgcctg tcgccaaaag tgggttcgct gattttttgc cataaatatt aagctatccg
     1141 gttgagatca gttgggccaa aaaaaagtgc tcatctctac actaaatcga gggcagttaa
     1201 agtatagagc gtttcatttt gtagcagaag ttctccgatt gaaataaatc catcttgctt
     1261 tggaaaaaat tggaaatttt tgatgacttc cgttcaacac tgctgctaga atacaaagaa
     1321 tttcccttca gtcacctcat tttaccaccc atccatccta tatcactgaa cgccctatgg
     1381 catttcggcg agttcttcac tggtttcggc gcgactttcg acttgaagat aacacggcgt
     1441 tgctgactgc ctgtacccag gcagaagaga tcatcccggt ttacattttt gacccaggct
     1501 ggctttcacg ccccgatgcc agccctgaac gaggagtgtt tttgctcgac agcttacaca
     1561 gcctgcagca gaaactcagt caggttggaa gctatctggt tgttcgccag ggtgacccgg
     1621 cacaggtact tgaccaactt ctgaatgaaa ctcaagcaga agcgatcttt gccaatcgaa
     1681 ttgttgatcc gtattttcga actcaaggtc gtcaactcga ccagcgcttg cgtcagcgtg
     1741 ggtgcgaggt acgaagttgc caggatgtga tgttgcatcc gccacaaagt gttctgacca
     1801 aagccggaac gccctatacc gtctttacac cgtataagaa taatgccctg acagttccag
     1861 ttcaaacacc gcaacctgca ccgcaccgca tcacaacccc agccggcatt gcctctgaac
     1921 caatcccgac gatccaggag cttggatttt caacttcggc catactcccc aaaggtggaa
     1981 ttgaggaagc ccatcgccgc ctggacgaat ttgcggcctc acgtctcaac cagtatgcca
     2041 atcagcgcga cctgccgtac ctcgaaggga catcgcggct atcaccatac ctgcactttg
     2101 gctgtgtgtc gccacgccgt gttttctgga ccgcctttca ggcgttgcat cagacccaac
     2161 cggccactgc ccctcgggaa tcagttgaaa cctggatctc agaacttttg tggcgagatt
     2221 tctatctgca aattctctac cacttcccac acgttgaaac tggtgccttc aaacctgttt
     2281 acaacgatct ggtctgggat acctcagaag agaattttgc tcgctggtgt cagggtcaga
     2341 ccgggtttcc aattgtagat gccgggatgc gccagttaaa cacaaccggc tggatgcata
     2401 accgggttcg catgatcgtg gcgtcatttc tcaccaaaga tttgctgatc aactggcagt
     2461 ggggcgaacg ctattttatg caacatctgg ttgatggcga cctcgcggcc aacaatggtg
     2521 gctggcagtg ggcagcctca accggcaccg acgctcagcc ttatttccgg atcttcaacc
     2581 cgatttcaca aggacagaaa tttgacccca aaggagagta tgtgagacgt tatatccctg
     2641 aattaaagca tgttccggag aagtggattc atactccgtg ggatataccg gctaaatatc
     2701 aagattcatt gcgatgccgt gtgggcattg attaccccaa accagttgtt gatcatgccc
     2761 ggcaacgcat gctggcactg gatatgtttc aacggg
//
LOCUS       DAOQDO010000656         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29893_length_2795_cov_2.222628, whole genome shotgun sequence.
ACCESSION   DAOQDO010000656 DAOQDO010000000
VERSION     DAOQDO010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29893_length_2795_cov_2.222628"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PKZ53_11190"
     CDS             complement(<1..791)
                     /locus_tag="PKZ53_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41046.1"
                     /translation="MNDSTHTSQVEREALLQELQALREENEQLRALESGYKRAMELLR
                     ERETRARMSAARKIVEATPIVENNFQSTPIGELLNAVLRVLPIGVWIIDRGGSILYEN
                     LAGQQMWAGVKQASSNIKGPQAVWSTETTRQVKAHEWAIARAITQSETSLNEEVVTIE
                     YLNGNRRSILNSAIPIWTESHTFAAALVMNQDVTDAQQASENLQRLNEFLEYRVYERN
                     QQFEVIYRDLKVEIAERKRTEEQLHLFRSAVVHAQDSIYILEPSLT"
     gene            complement(791..>2795)
                     /locus_tag="PKZ53_11195"
     CDS             complement(791..>2795)
                     /locus_tag="PKZ53_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ41047.1"
                     /translation="TPAASLIRGFFSEISAVWIWYLPQRRQWRTVGVLASSTVLALAA
                     GTSLNPASRHVVLLILCLFGVWLLIELIRLQVNQVPFAFRTTTEKFRYWGIICFTSVI
                     ICGLTLIGSTSLNLISRNLFRVFGWLESASGSGLDSFSDQMGASGSLILSRQSNLRLE
                     PTIVATVDGVKPPGYLRTQVFSTYTERGWETEKGQAQPIPDEWITTTDTTQEVSFPQT
                     PVPNQDPTPQTSPSILTRQVQVFADLHGVLPLSYGTTGFEVGKSINLKYARGPSIWCF
                     PGTSLNQYRISAPFPVQPNQVPQPPLSAVELTLNTALPPALATQLQPIAHQVVGSHRH
                     SPLEAARQVETFFQTQFVYSLDVKLNPQGDPIVDFIQNRRPAYCKYYASGMVLLLRSI
                     GIPARVAAGFYSQELNHTSRQWIIRQHHAHAWCEVYDQESHGWIAFDPTPPAAINYER
                     EQGFARWWKEKLTWLSLTGKQLIQAIAHLHLVNLLQKPWNYMKNAGLKVWGIGLTFAI
                     AGFLLYRLRFQISLNTLNLTWFLNPLRWIHPQPTALTAEDLLGEVFNAFQTAGLSIQE
                     SETITEFFHRVEQTLRVTIEPGPSTQNRDSVLDVPDALNQIRTFFETYQAIRYSPMTQ
                     AMLTTDHQQRLQMQAHLAVETIRRLNPDLRTARTAETLPITPS"
BASE COUNT          645 a          693 c          776 g          681 t
ORIGIN      
        1 gtcaggctgg gttcaagaat atagatgctg tcctgggcgt gaaccacggc tgatcgaaag
       61 agatggagct gttcttcggt gcgtttgcgt tcggcaattt ccactttcaa gtcacgatag
      121 atgacttcaa attgctgatt ccgttcatag acccgatatt ccagaaattc attgagccgc
      181 tggaggtttt cactggcttg ttgggcgtca gttacatcct ggttcatcac cagcgccgcc
      241 gcaaaggtat gggactcggt ccagatggga atcgctgaat tcaaaatcga ccgccggttg
      301 ccattgaggt attcaatcgt gacgacttct tcgttgagtg atgtctcact ttgggtaatg
      361 gcacgggcaa tcgcccactc atgggctttg acctgacggg tggtttcagt tgaccacacc
      421 gcctgtgggc ccttgatatt gctcgacgcc tgtttgacgc ccgcccacat ctgctgcccg
      481 gccaggtttt catagagaat gctcccccca cggtcaatga tccagacgcc tattgggaga
      541 actcgtaaga cggcattgag caactcgcca attggtgttg attggaaatt gttttcaacg
      601 atgggggtgg cttcgacaat tttccgggct gccgacatgc gagcgcgggt ttcccgttca
      661 cgcagcaatt ccatagcccg cttatagccg gattcaagcg cccgcaattg ctcgttttct
      721 tcacgcagtg cctgaagttc ttgtagcaac gcttctcgtt caacttgaga ggtgtgggtt
      781 gaatcgttca tcagctcggg gtgatcggta gagtttccgc ggtgcgggcg gtgcggaggt
      841 cagggttcaa gcgccgaatc gtttcaacag ccagatgcgc ctgcatctgt aaccgctgtt
      901 ggtgatcagt ggtcaacatc gcctgggtca tcggtgagta acgaattgcc tgataggttt
      961 caaaaaacgt gcgaatctgg ttgagtgcgt ccggtacatc aagcactgaa tcccggtttt
     1021 gggttgatgg tccaggctct atggtaaccc ggagcgtttg ttcaacccga tgaaaaaact
     1081 ccgttatcgt ttcagactcc tgaatactca gcccggcggt ctgaaacgcg ttgaatactt
     1141 ctcccaataa gtcttcggct gtcagtgcgg tcggttgagg gtggatccag cgaagcgggt
     1201 tcaaaaacca ggtcagattg agtgtattga gcgaaatttg aaatctcagt ctatataaca
     1261 gaaaacccgc gatggcaaaa gtcaggccga ttccccagac cttcaagcca gcatttttca
     1321 tgtagttcca gggtttttgc aacagattca ccagatggag gtgagcaatc gcctggataa
     1381 gctgtttccc ggtcaggctc aaccaggtaa gtttctcttt ccaccaccgg gcaaacccct
     1441 gttcccgctc atagttgatt gcggctggtg gcgttgggtc aaacgctatc cagccgtgtg
     1501 actcctgatc atagacttca caccaggcat gggcatggtg ctggcggata atccactgcc
     1561 gactggtgtg attgagttcc tggctgtaaa aaccagccgc cacccgggct ggaatcccga
     1621 ttgaccgtag cagcaacacc atcccggaag catagtattt acaataggct ggccgccgat
     1681 tctgaataaa atcaacgatt ggatcccctt gtggattgag tttgacatcg agtgaataca
     1741 caaactgggt ctggaaaaat gtttctacct ggcgggcggc ttccagtggt gagtgccggt
     1801 gagaaccaac gacttgatgg gcaatgggct gcaattgtgt ggccagtgca ggcggaagag
     1861 cggtattgag tgtcagttca acggctgaaa ggggtggttg tggtacctgg ttgggttgaa
     1921 cgggaaacgg ggctgaaatc cggtactggt tgaggctggt gcctggaaaa caccagatgg
     1981 atggtccccg ggcatatttt aagttgatgc ttttcccaac ctcaaaccct gtggtgccat
     2041 aactcaatgg caataccccg tgtaaatcag caaatacctg aacctgccgg gtgagaatgc
     2101 tgggtgaagt ctggggagtc ggatcctggt ttgggacagg cgtttgtggg aatgaaacct
     2161 cttgtgtcgt gtcagtcgtg gtaatccatt catctggaat gggttgtgcc tgaccctttt
     2221 cagtttccca gccgcgctcc gtatacgttg aaaagacctg ggtgcgcaaa taccctggtg
     2281 gctttacacc atcaaccgtg gccacgatgg tcggttcaag gcgcaggttg ctttggcgcg
     2341 aaagaatcag acttcctgac gcacccatct ggtccgaaaa gctatccaga ccggatccag
     2401 aagctgattc aagccatcca aacactcgaa acagatttcg gctgatcaga ttgagcgagg
     2461 tacttccaat gagcgtcaac ccacagatga tcacactggt gaaacagatg atgccccagt
     2521 atctaaattt ttcagttgtg gtgcgaaaag cgaaaggcac ctggttgact tgcagccgga
     2581 tcaactctat caggagccat accccaaaaa ggcaaaggat caagaggaca acgtgccggc
     2641 tggcaggatt caaagacgtt cccgctgcca gagccagaac tgtgctgctg gccagcactc
     2701 ccacagtgcg ccactgccgg cgttgtggga ggtaccaaat ccacaccgca ctgatttcag
     2761 agaaaaaacc ccgaatgagg ctggcggctg gggta
//
LOCUS       DAOQDO010000657         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29941_length_2792_cov_3.994885, whole genome shotgun sequence.
ACCESSION   DAOQDO010000657 DAOQDO010000000
VERSION     DAOQDO010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29941_length_2792_cov_3.994885"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PKZ53_11200"
     CDS             complement(<1..1229)
                     /locus_tag="PKZ53_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HNJ41048.1"
                     /translation="MSIKPILAPKNEQRLGLVLTPQMRQRIEMLSMTSLELSDMVSTE
                     MVANPVLEEVLPNEPAEGLTVTDEQLAFGERPIDGAPQATDPTLDFPLDYQSESVSLY
                     AAEMPNTAPEPQIDEINNVQEPEVNPDPFQEIDFGAEFENYLDPGYKTFEHEDSDQPS
                     FENLLTKKPTLAEHLLWQLNLTRTPEEIRAAAEAIIGSLNTNGHLADPLEEIAALGPW
                     SMTVVEKAKRLVQSLDPVGVGSTNVLDCFLIQLELKGFANHLATQLVQHHFNRLQQYR
                     WPDLAKELGVSIDEIAKAVTLIVKLEPYPGRQFAHDEAYPIQPEIFIEKIDDEYYIHF
                     NDDGIPQLRINSHYRRLLESNQASKEEREYVRECFRSAVDLLKNIEHRRQTIYKVCQA
                     IVERQRAFLDGGVEYLKP"
     gene            complement(1296..2111)
                     /gene="lptB"
                     /locus_tag="PKZ53_11205"
     CDS             complement(1296..2111)
                     /gene="lptB"
                     /locus_tag="PKZ53_11205"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNJ41049.1"
                     /translation="MSDTHHKTSSNATSSDSSNQTPTQHTDQPTPGDSLRVEGLNKAY
                     RGREVVRDVSIHIQQGEVVGLLGPNGAGKTTTFYMIVGLEQPDKGVITLGDQAITSLP
                     MYLRARKGISYLPQEPSIFRKLTVEKNILAILETLRVPGQIRAEILESLLSELDIAHV
                     RHTPGYALSGGERRRAEIARCLVIEPKFILLDEPFAGIDPKAVVEIQNIISRLKARNI
                     GVLITDHNVRETLAITDRAYIISEGKIFRSGTPQSLTSDEEVRKIYLGENFRM"
     gene            complement(2131..>2792)
                     /locus_tag="PKZ53_11210"
     CDS             complement(2131..>2792)
                     /locus_tag="PKZ53_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNJ41050.1"
                     /translation="SQESTGRATPFGKTKSPVFLTAQQARVNTQTGKATYQGEARAWQ
                     DDNFIRGDVIELFQTEKRMTATGHVSSALYQMERTNPKGQKQVIPIFGSSEFFSYNDS
                     KRQARYERNSRLIQGEDTLSADSTDIYLAATTNQVEHLIAQGKVVLTQPQRRGTGDQA
                     EYTASDDKFVLVGNMARVEDSAQGTTTGPRLTFLRSDGSVLATDQRQTQRIRTTHKIS
                     K"
BASE COUNT          667 a          625 c          674 g          826 t
ORIGIN      
        1 ggcttgaggt attcaactcc accatcaaga aaggcccttt gtcgttcgac aatagcctga
       61 caaactttat agatggtttg tcgccggtgt tcgatatttt tcagcagatc gactgccgag
      121 cgaaagcatt ctctcacata ttcccgttct tctttcgaag cctggtttga ttcgaggagc
      181 cggcgataat gggaattgat gcgaagctgt ggaattccat catcattgaa atggatgtaa
      241 tactcgtcgt cgatcttttc aataaagatt tcaggttgga ttgggtacgc ttcatcgtga
      301 gcaaattgcc ttccgggata gggctctaac tttacaatca gcgtaactgc cttggcgatt
      361 tcgtcaattg aaactcctaa ttcttttgcc aggtccggcc accggtattg ttgtaatcgg
      421 ttaaagtggt gctgaaccag ttgagttgcc aggtggttgg caaatccttt cagttcaagc
      481 tggatcagaa aacaatcaag aacatttgtt gatccaacac caactggatc aagtgattgg
      541 accaggcgtt tggctttctc caccacggtc attgaccagg gacccaaagc cgcaatttct
      601 tcaagtgggt cagccagatg cccgtttgta ttgagactac cgataatcgc ttcggctgca
      661 gccctgatct cctcaggtgt tcgagtgaga tttaattgcc agaggagatg ctcggccagt
      721 gttggttttt ttgtcagtaa attttcaaaa gatggctggt cggaatcttc gtgctcaaat
      781 gttttgtatc ctggatctag atagttttca aactcagcac caaagtcaat ttcttgaaat
      841 ggatcaggat tgacttcagg ctcctggacg ttgttgattt catcaatctg gggttcaggg
      901 gctgtatttg gcatttcagc ggcatacaac gaaactgatt cagactgata gtcaagtgga
      961 aaatccaggg tggggtctgt cgcctgtggt gccccatcaa ttggtcgttc gccaaacgcc
     1021 agttgctcat cagttacggt tagcccttcg gctggctcgt ttgggagtac ttcctctaaa
     1081 accggatttg ccaccatttc agtcgatacc atgtcgctca attcaagtga cgtcatgctt
     1141 aacatttcaa tgcgctgtcg catttgagga gttaagacca atccaagtcg ttgttcgttt
     1201 ttgggagcaa gaatcggctt aatagacata ggacatcttc acttgatcaa aaaaatggaa
     1261 aagcagatta atgaaaggga ggtgcttgag ggaggctaca ttctaaaatt ttcgcctaga
     1321 taaatcttac gcacctcttc atctgatgtc aggctctggg gggttccaga tcggaatatt
     1381 ttgccctcgc ttattatata ggcccggtca gtaattgcta gagtttcgcg aacattgtgg
     1441 tcagtaatca gaactccaat attgcgagcc ttcagccggg agatgatatt ttgaatttca
     1501 acgactgctt tggggtcaat tccggcaaac ggttcatcca gcaatataaa tttgggttca
     1561 atgacaaggc aacgggcaat ttcagctcgt ctacgttcgc ctccagaaag ggcatatcca
     1621 ggtgtatgcc ggacatgggc tatatcaagc tcactgagaa gtgattccag gatttcagcg
     1681 cggatttgac caggaacacg cagtgtttca agaatggcca ggatattttt ttcgacggtt
     1741 agtttccgga aaatggatgg ttcttggggg agataactga tgcctttccg tgctcgaaga
     1801 tacattggca gggaggtgat tgcctgatca cctagtgtaa tcaccccttt atctggctgc
     1861 tcaaggccta cgatcatgta gaaagtcgtt gtttttccag ctccatttgg acccagcaat
     1921 cccacgactt cgccctgctg aatatggatt gaaacgtcgc ggacaacctc acgtccccga
     1981 tacgctttat ttaatccttc tacacgaagg gaatcgccag gtgtgggctg atcggtatgc
     2041 tgagttggag tttgatttga agaatcggag gaagttgcgt tagatgatgt tttgtgatga
     2101 gtatctgaca caaggcccac ttcaatctga ctatttgctg attttgtgag ttgtacggat
     2161 tcgttgggtt tgtcgttgat cggtggctaa cacactacca tccgacctta agaaagtcaa
     2221 tcgaggtcct gttgtcgtac cctgtgccga atcttcaacc ctagccatat taccaactaa
     2281 gacaaatttg tcatctgatg ccgtgtattc tgcttgatca ccagtcccac gacgttgagg
     2341 ttgggtgaga actacttttc cttgggcaat gaggtgttcc acctggttag ttgtcgccgc
     2401 aagataaata tccgtactat cggcgctcag ggtatcttct ccctggatca ggcggctatt
     2461 ccgctcataa cgcgcctgac gttttgagtc gttataagaa aagaactcac tgctcccaaa
     2521 aattgggatc acctgttttt gtccctttgg attcgttcgt tccatctggt aaagagcgga
     2581 tgacacatgt ccagttgctg tcatgcgttt ctcggtttga aaaagctcaa tgacatcacc
     2641 tcgaataaaa ttatcatcct gccaggcccg cgcttcaccc tggtaggttg ccttcccggt
     2701 ttgtgtgttt acacgggctt gttgtgccgt taaaaaaact ggcgactttg ttttcccaaa
     2761 tggtgtcgct cggccagtac tttcctggct at
//
LOCUS       DAOQDO010000658         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_29985_length_2790_cov_2.986837, whole genome shotgun sequence.
ACCESSION   DAOQDO010000658 DAOQDO010000000
VERSION     DAOQDO010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29985_length_2790_cov_2.986837"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1113
                     /locus_tag="PKZ53_11215"
     CDS             178..1113
                     /locus_tag="PKZ53_11215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ41051.1"
                     /translation="MKLFTTFLAISLIISTLSPGQPSVLAHSNSPHRQKGGSIEDAII
                     DAVGVAVPLGKEAVRSLVKPILDTVTITPQQEMQIGNQFFQNIKKKLGSKLDRDQREV
                     AYVTSIGNVLATNVKRKGIKYKFHVVEDRLPNAFAIAGGHVFIYRGLLETVIENEAQL
                     AAVIGHEISHVDAEHTVDFYKPVVAASQLPFADVSVLIAALGSKLLTFSYSEVQESEA
                     DNLGTVLAFKAGYEPAEGKNMQQRLGSLKSTGATDPLTGLADTVFRTHPPSQKRAAAI
                     DALSKKLHTQKPNQKTYVGEANYRQRIPMTQKILE"
     gene            1281..2582
                     /gene="eno"
                     /locus_tag="PKZ53_11220"
     CDS             1281..2582
                     /gene="eno"
                     /locus_tag="PKZ53_11220"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNJ41052.1"
                     /translation="MLTIEHIHGREILDSRGNPTVEAEVQLSDGTWGMAAVPSGASTG
                     ENEALELRDGDKGRYLGKGVLKAVANVNEQIAKALYGFNALDQIGVDRTMLQLDGTKT
                     KAVLGANAMLAVSLATARAAANALEIPLYRYLGGAHARTLPVPMMNILNGGAHADNNV
                     DFQEFMIVPMGASSFAEALRWGAEVFHTLKGVLKKRGYSTAVGDEGGFAPSVKSNVEA
                     IEVVLEAITQAGYKPGDQIFIALDPAASEFYIDGSYVFKKSDGSKKTSEEMVDYWVNW
                     VDQYPIISIEDGMGENDWDGWSLMSQKLRDRIQLVGDDLFVTNVEYLRKGIDLNVANS
                     ILVKVNQIGTLSETMDTVELARTRRYGAIISHRSGETEDTFIADLAVALNAGQIKTGS
                     ASRTDRVAKYNQLLRIEEELGASALYAGCGGFYQLQTKKSE"
BASE COUNT          740 a          708 c          686 g          656 t
ORIGIN      
        1 ttggttcttg gttcttggtt cttggttctt ggttcttggt tcttcgaagg tattgattcc
       61 aaatcactaa gcaccaggta ttaagcacca agcaccaagc accaagcacc aagttccaag
      121 taccaagcac caaacaccaa gttccgaggc acgtacattc atccaggagg gaattttatg
      181 aaactcttta ccacattttt agccatttcc ctcatcatca gcacactcag cccaggccaa
      241 ccttctgttt tagctcattc caactcacca catcgccaaa aaggtggatc cattgaagat
      301 gccatcattg atgcggttgg cgtcgccgtt ccacttggca aagaagccgt ccggtcactg
      361 gtcaaaccaa ttctggatac cgtcaccatc acgcctcaac aggaaatgca gatcggcaac
      421 cagttttttc aaaatattaa gaaaaaactt gggtcaaagc ttgatcgaga tcaacgcgaa
      481 gtggcctacg tgacctcgat tggcaatgtg ctcgcgacca atgtcaaacg caaaggcatt
      541 aagtacaaat ttcatgtggt tgaagaccgt ctccccaatg cctttgccat cgccggggga
      601 cacgttttta tctatcgtgg attacttgaa acggtgattg aaaacgaagc ccaactggcg
      661 gcagtgattg gacatgaaat ttcacatgtg gatgccgagc ataccgtgga tttctacaag
      721 ccagtggtgg cagcttcaca attaccgttt gctgatgtct cggttctgat tgctgcactt
      781 gggtcaaaac tgcttacctt ttcttatagc gaagttcagg aaagcgaagc tgacaatctt
      841 ggaacggttt tggctttcaa agccgggtat gaacccgccg aaggcaaaaa tatgcagcaa
      901 cggctggggt cactcaaatc cacgggtgca actgatccct tgaccgggct ggccgatacg
      961 gtttttcgaa cccaccctcc gtcacaaaaa cgcgcggcgg ctattgatgc actttcgaag
     1021 aaacttcaca cccaaaagcc aaaccagaag acctatgttg gcgaagccaa ttatcgccag
     1081 cgcatcccga tgacccagaa gattttggag tgacgggatc tggtgcttag tacctggtgc
     1141 ttggtgctta gtacctggcg cttagtgctt tgggatcaat acttaccaag taccaagtac
     1201 caaaaaccag gcaccaagca ccctttctga acgacaaatt gccctctcaa ctcaatactt
     1261 tcaaggagtc acatcctctt atgctgacaa ttgaacacat tcatggacgg gaaattcttg
     1321 attcccgtgg aaatccaacg gttgaagccg aagtccagct ttcagatgga acctggggaa
     1381 tggccgccgt gccgtcgggg gcttcgacag gtgaaaacga agcgcttgaa ctccgggacg
     1441 gcgacaaagg ccgctacctt ggaaaagggg tcctcaaagc cgttgccaat gtgaatgaac
     1501 aaattgccaa agcgctgtat ggtttcaacg ctctggacca aattggcgtg gatcgaacca
     1561 tgctccagct tgacggaacc aaaaccaaag ccgtacttgg cgccaacgcc atgctggccg
     1621 tttcgctggc aaccgcccgg gcagcagcca atgcactgga aattccactc taccgctatc
     1681 ttggtggcgc ccacgcccga acgctgccag tacccatgat gaacattctc aacggcggtg
     1741 cccacgccga taacaatgtt gattttcaag agtttatgat tgtcccaatg ggagcgtctt
     1801 catttgctga agcgcttcgc tggggcgccg aagtctttca taccctgaaa ggcgtgctca
     1861 aaaaacgtgg ctattcaacg gcagttggcg atgaaggcgg atttgctccg agcgtcaaat
     1921 caaacgttga agcgattgaa gtcgtccttg aagccatcac gcaggctgga tataaacccg
     1981 gtgaccagat tttcattgcg ctcgacccgg cagcgagcga gttttacatt gatggctcat
     2041 atgtgtttaa gaaatcagac ggttccaaga aaacatccga ggaaatggtt gactactggg
     2101 ttaactgggt tgaccaatac ccgattattt ccatcgaaga cgggatgggt gaaaatgact
     2161 gggatggctg gtcactgatg agccagaaat tgcgtgaccg cattcaactg gttggtgatg
     2221 acctgtttgt gaccaacgtt gaatacctgc gcaaagggat tgacctcaat gtggccaatt
     2281 ccattctggt caaggtcaat caaatcggaa cattgagcga aacaatggac acggtcgagc
     2341 ttgcccggac ccgccgctat ggcgcgatta tttcacatcg ttccggtgaa accgaagaca
     2401 cgtttattgc cgatctggcc gtcgcgctca atgccggtca aatcaaaacg ggttctgcca
     2461 gccggactga ccgggtggcc aaatacaatc aattgctcag aattgaagaa gaacttggtg
     2521 cttcagccct ctatgctggc tgcggtgggt tctatcagtt gcagaccaag aagagcgaat
     2581 agccaaacag ggctgagggc ggcgatgaaa gagatgaggg atgaaggatg agggatgaaa
     2641 ccaagggatg agggatgaaa taaaaccaat tcttcagccc gatgtcttca gcccgatgtc
     2701 ttcagcccca agcccgtctt cttcagccct cagttttcag caaatcggct tcaaccagtt
     2761 gatgaatgcc tggcaaattt gtcttcaact
//
LOCUS       DAOQDO010000659         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30017_length_2788_cov_2.844859, whole genome shotgun sequence.
ACCESSION   DAOQDO010000659 DAOQDO010000000
VERSION     DAOQDO010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30017_length_2788_cov_2.844859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKZ53_11225"
     CDS             <1..658
                     /locus_tag="PKZ53_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNJ41053.1"
                     /translation="LDDIRQTRVDEWADELASIDDDEALKDLWPLAKNYVYSALHQKV
                     EERWQAGLSPEAERNPALHPFVKSEMSFALRTDAAMILGSVDKLLISEHQDGLHVHVI
                     DFKTNTLWKSRAGYDTELAEKAAMYRIQMQAYSLAAWWLLPNVKRVEATLHFLHPNLE
                     YHFPADQLSEDACHRAVLNVVKKIRNVRVMESTQFEANPGARCLNCRFAEVCTDAVRM
                     "
     gene            744..1139
                     /locus_tag="PKZ53_11230"
                     /pseudo
     CDS             744..1139
                     /locus_tag="PKZ53_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018398236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XisH family protein"
     gene            complement(1298..1453)
                     /locus_tag="PKZ53_11235"
     CDS             complement(1298..1453)
                     /locus_tag="PKZ53_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41054.1"
                     /translation="MACLSSVEDHHADAVSSLPVSVCLWFGTETSLVEDSRTGRITRF
                     HQFTHTG"
     gene            1465..1617
                     /locus_tag="PKZ53_11240"
     CDS             1465..1617
                     /locus_tag="PKZ53_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41055.1"
                     /translation="MSKRPAEAFPVKVEIQGMRAGVWEAVAHISAAGGGRQTVPGRTQ
                     DTGRPP"
     gene            1797..2135
                     /locus_tag="PKZ53_11245"
     CDS             1797..2135
                     /locus_tag="PKZ53_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XisI protein"
                     /protein_id="HNJ41056.1"
                     /translation="MDSKLTHYQQLLKTIVTRQSQVISSDPNCELLPVCDLENNQFLV
                     IDTGWNVAGRVHNLVFHARIKNDKIWIEWDGFENGVTEELLDAGVPHQDIVLAFYRPE
                     RRKLTDFAVA"
     gene            2173..2505
                     /locus_tag="PKZ53_11250"
     CDS             2173..2505
                     /locus_tag="PKZ53_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012142898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41057.1"
                     /translation="MVSVCHRFQVQPIFPDPAEKVGIALQTIGTLPIHALRIFPEGGT
                     SGWYIWAGGDMSTDPDFFQPLHICHLDQYCPELVPYLGLPPGWRVLLAPNHEDVWFDP
                     ALVKAEKS"
BASE COUNT          724 a          682 c          738 g          644 t
ORIGIN      
        1 gttggatgac atccgacaga cccgcgtaga cgaatgggcc gacgaactgg cctcaattga
       61 cgacgacgaa gcgctcaaag acctctggcc gctggcgaaa aattacgttt acagcgcact
      121 ccatcaaaaa gtcgaagaac gctggcaggc agggttgtcg cccgaagccg aacgcaaccc
      181 ggcgctccat ccatttgtga agagcgaaat gagttttgcc ctgcgcaccg atgcggcgat
      241 gattttgggt tcggtagata aattgttgat ttcagagcat caagatgggc tgcatgtcca
      301 cgtgattgac tttaagacca acacgctctg gaaaagtcgc gccggatacg acaccgaact
      361 ggccgaaaaa gccgcgatgt atcgcattca aatgcaggca tattcgctgg cggcgtggtg
      421 gttgctgcca aacgtgaagc gcgtcgaagc aacgctccat ttcctgcatc cgaacctcga
      481 ataccatttc ccagccgacc agctttccga agacgcctgt caccgcgccg tgctcaacgt
      541 cgtgaagaaa atccgcaacg tccgggtcat ggaaagcacg caatttgaag ccaaccccgg
      601 cgcccggtgc ttaaactgcc gttttgctga agtctgcacc gacgccgtgc ggatgtagac
      661 tacagaagaa aaaatagttg ttggttagat aaataaagat acagcaaaat gacggtggaa
      721 tgttttttac cgagtgaata cctatacctc aacgtgatct ttaccacgag aatgtcaaaa
      781 atgcgcttat caaggatggc tggacaatca cggacgatcc attccggatt caatataagg
      841 gagacacact ttttgctgac cttggggcgg agaaacccat tgccgcgcag ctcgggacac
      901 ggaaaattgt ggttgaaata aagatgttca atgctccttc actctacaca gagtttgaac
      961 gggcgcttgg tcaatacatt ctttacagga cactaatggc cgttgtttat ccaaaccatg
     1021 agttattttt aggtgttcct gaagatatcc aggccgactt tttcaaacgt gaagcagttc
     1081 ggttgacact gacggctcaa cgcatcaatg tgcttgtgtt caatcctgaa caggaggagc
     1141 gattagatag cctaaaatgt gatgaaagct gttcaaacac gctcttgatg aagatgtttt
     1201 ctgaatcgcc tccagtgttt gaggtggact tgatctttca aaatcaatgg tttatggtgg
     1261 ctggcggagg gatgttccct tcgtgagcag gttcagttca gccggtgtgg gtaaactggt
     1321 ggaagcgggt gattcgtccg gtgcgggaat cctcgacgag actggtttca gtgccgaacc
     1381 acaagcacac cgataccgga agtgaactga ccgcgtcagc gtggtgatct tcaaccgaag
     1441 acagacacgc catcgccggt tggaatgtcg aagcggccag ccgaagcctt tccagtcaaa
     1501 gtggaaatcc aggggatgcg agccggtgtg tgggaagcag tggcgcacat ctcggcggcg
     1561 ggtgggggga gacagactgt tccgggcagg actcaagaca ctggacgacc accgtgaccg
     1621 gacgtggtgg gaggcggcag cgccgggaga tgggagcgat gagaaaactt gtgggtaagc
     1681 gaagcatcgc gtgaaaccat ttcagtcaat ctgagagcga aacttccggc ttcccaggcc
     1741 ggcaaaacat agtctgaaca catttgagta tgttttgagg aacaggtaat tacacaatgg
     1801 attcaaagtt aacccactat cagcaactcc tcaaaacgat tgttacccgg cagtcccagg
     1861 tgatttcgag cgatcccaat tgtgaattac tcccagtgtg tgatttggaa aacaaccagt
     1921 ttttggtgat tgatactgga tggaacgtcg ctgggcgagt ccataacctt gtgttccacg
     1981 cccggatcaa gaacgacaaa atctggatcg aatgggacgg ctttgaaaat ggagtcaccg
     2041 aagaactact tgatgctggc gttccacatc aagacatcgt tttagccttc tatcgtcccg
     2101 aacggcgcaa gttgaccgat tttgctgtgg cctgagttga tttgaaaaaa agaacgtact
     2161 ccaatggaat caatggtttc tgtttgccac cggtttcaag tccagccgat atttcctgat
     2221 ccagccgaaa aggtgggaat tgctttgcag accatcggaa ccttaccgat tcatgcgctt
     2281 cggatttttc cggagggtgg cacttcaggc tggtacatct gggcaggcgg agacatgtca
     2341 actgacccgg acttctttca accactgcat atttgccacc ttgatcaata ttgtcccgaa
     2401 ctcgtgccct atcttggatt accacccgga tggcgagtgt tattggcacc aaaccacgaa
     2461 gatgtctggt ttgatccggc gcttgtcaaa gcggaaaagt catagcttga tcttggggac
     2521 atggttgaaa tggcgcatca atccagccgc ctcaatactg gaattcactg aaaatagatg
     2581 tcaaccgctg ccgtagcctg accacaggga cctgacatgc gctgcattcg ccacactttc
     2641 tgtttctcct gaatgctgaa gagaaagaca ccttcccgct cacaaacccg gtcgtaagtc
     2701 tcaatggaga ctctcccttt gaaccgaacg acaagcgcaa tctcggcctt tcaaggccga
     2761 gtcaagcgag tccgatcagg tgttgaga
//
LOCUS       DAOQDO010000660         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30031_length_2787_cov_2.909224, whole genome shotgun sequence.
ACCESSION   DAOQDO010000660 DAOQDO010000000
VERSION     DAOQDO010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30031_length_2787_cov_2.909224"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKZ53_11255"
     CDS             complement(<1..620)
                     /locus_tag="PKZ53_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HNJ41058.1"
                     /translation="MLAANHKNSDLPPIWVISLRRSTERRAHITQHLKEHNLPFEFFD
                     AIDGQTLTPDQLACYDAQQACQCMGRELVPSEIAIALSHLTLYQRMVDQNLDEVLILE
                     DDVVLKPEFLEILSRKSSFPKGWELVLLCHTWGILSAWYRQPIFRTTEVVRFSRSAVS
                     SAAYLLRQSGARKLLVAGYPIRVPSDHLTGGKIKTNVNLYGISPACA"
     gene            complement(836..>2787)
                     /locus_tag="PKZ53_11260"
     CDS             complement(836..>2787)
                     /locus_tag="PKZ53_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41059.1"
                     /translation="ATGDFQTAGNSPARSIARWNGQRWSPVGAGLTDKGRCLTVFDGK
                     LVCGGNFRGIGDQYFRRVAAWDGETWTPLGRGLNNWVEGLTVWNNHLVAGGWFNQSRE
                     GGPVGLAVLSSDSPPVPGQWGETDTSTPDKIIWPDTPYRYEDQVIQWVGAHNPRAQWM
                     KLGCENPRNWDFLRHPMGKPPDTTYLIWRYDRPLPEGTKLLIEGDFPHARFFSIGVNS
                     PSDTNFPVIGDGRGIQDVMIVDEDIIPDPGHFNPFVPGAYRQTPKRHYHVTVELQTGL
                     PETLNRDAVIPPYRDSGNLRFGGPYSINESGVSGPYLWVRIVQPDRYDPFGGVEPPVI
                     RIQFPGAEPVLAPVSRLMAVDEGVFLPRYSLQENPAQADGFSRQELEARAQQEQRARL
                     ELTQAGPVGRSSQEIHRIFSEGQTVRLFKVFQRPYYLGWLSRYGDEPWACTAWPNQYL
                     SLYRLGPYQPVPLNSETSSGANVCASVLATSVNLQSLDEVVVIEGKAPTSPQTLSGNK
                     LVQAGTQVRYWSVVFLAGSSADRKVPVISLTDEDIVLDREGKFKIVIGEAGHRPANAF
                     PRFGVTWRPWPVGSTLDVVFQYGVTSPQVWENAPQRILWSEADYCFGDPAGTGAANRQ
                     LLKARMGVFCPQIRYVTKDMFERMH"
BASE COUNT          682 a          789 c          712 g          604 t
ORIGIN      
        1 gcacaggccg gtgatatgcc atacagattc acattggtct ttattttgcc tcccgtgaga
       61 tgatcggaag gaacacgaat gggatagcca gccaccagga gtttccgggc gccgctctgc
      121 ctgagcagat aagcagccga actcaccgca cttcgggaga accttacaac ttctgtggtg
      181 cgaaaaatcg gctggcgata ccaggctgag aggatccccc aggtatggca taacaacacc
      241 agttcccatc cttttggaaa actggatttc cggctcagga tttccagaaa ttccggcttc
      301 aaaaccacat catcttccaa aatcaagact tcatccagat tttggtcaac catgcgctgg
      361 tacaacgtca gatgtgacag cgcaatggca atttcgctgg gcaccaactc cctgcccata
      421 cactggcaag cctgttgggc gtcatagcaa gccagttggt caggggtcag agtttgccca
      481 tcaatcgcat cgaagaattc aaatggcaga ttgtgttctt tgaggtgctg ggtgatatga
      541 gcccggcgct cagtcgaacg acgcaggctg atcacccaaa ttggagggag atcagagttc
      601 ttatggtttg cggcaagcat ttcagaactt cctttgtaca cattgaacta cagggctgac
      661 ctggaaaacg gtgcagccac tcaactttaa acccggtcca ggaatatgga ttatagattc
      721 tttccttttt ttccaggtca aggtgaggaa tggtggggaa cgttcaggta ttcagaatgc
      781 tattgggtcc gctcatcaaa acgcaacaca ttttccagat gccggagttg catccttagt
      841 gcattcgctc aaacatatcc tttgtaacat accgaatctg aggacaaaac acccccattc
      901 tggcttttaa gagctggcga ttggcggcac cggtcccggc tgggtcgcca aaacaataat
      961 cagcttcaga ccataaaatc cgctgcgggg cattttccca aacctgtggt gacgtcacac
     1021 catactggaa aaccacatcc agggtactcc ccaccggcca gggccgccac gtgacgccaa
     1081 agcgcggaaa ggcattggct gggcgatgtc ccgcctcgcc aatcacaatt ttgaacttcc
     1141 cttcccggtc caggacgatg tcttcatccg tcagactgat gaccggaact ttccgatctg
     1201 ccgaactgcc agccagaaaa accacagacc agtatcggac ctgtgttccg gcctgcacaa
     1261 gcttgttccc tgacagtgtt tgaggcgacg ttggggcttt tccctcaatc acgaccactt
     1321 cgtcaaggct ttgtaagttg accgaggtag ccagcaccga agcgcacaca ttggcaccgc
     1381 ttgacgtttc agaattcaac ggcacgggct gatagggacc caggcgatag aggctcaaat
     1441 attggtttgg ccaggcagtg caggcccacg gttcatctcc gtaccgggac agccagccca
     1501 gatagtaggg tcgctgaaaa actttaaaca gcctgacggt ttgaccttct gaaaaaatcc
     1561 gatgaatttc ctggcttgaa cggccaacag gtccagcctg ggttagttcc agccgggctc
     1621 tttgctcctg ttgggcacga gcttcgagtt cctggcgtga aaaaccatcc gcctgagccg
     1681 ggttttcctg gagcgaataa cgcggcagaa agacaccttc atcaaccgcc atcaaccggg
     1741 aaactggcgc cagtacaggt tcggccccag gaaactgaat ccgaatcacc ggcggctcga
     1801 caccaccaaa cgggtcatag cggtcaggct ggacaatgcg aacccagaga tacggcccgg
     1861 aaacaccact ttcattgatt gaatacggac cgccaaatcg cagattgcca gagtcgcgat
     1921 agggtggaat caccgcatcc cgattgagcg tttcaggcaa cccagtttgc aattcaacgg
     1981 tgacgtggta atggcgcttt ggggtctggc gataggcccc tggcacaaag ggattgaaat
     2041 gaccaggatc cggaatgatg tcttcatcca caatcatcac atcctggatt ccccgcccat
     2101 caccaatcac cgggaagttt gtgtcagatg gagaattcac cccgatactg aaaaaccggg
     2161 catgggggaa atcaccttcg atcaataatt tggtaccttc agggagcggt cggtcatagc
     2221 gccagatcaa ataggtggta tccggaggct tgcccatcgg gtgccggaga aaatcccagt
     2281 tgcgcgggtt ttcacacccc agtttcatcc actgagcgcg tgggttgtga gccccaaccc
     2341 attgaatcac ctgatcttca taccgatagg gcgtgtcggg ccagatgatt ttgtccggtg
     2401 tcgaagtatc agtttcgccc cattgtcctg gaacgggtgg cgagtctgaa ctcaaaaccg
     2461 ccagccccac cgggccgcct tcgcgtgact ggttaaacca gccgccagcc accagatggt
     2521 tattccagac cgtcaagcct tcaacccagt tgttcaatcc acgtccaagc ggcgtccagg
     2581 tttccccatc ccaggcggcg actcgtcgga agtactgatc accgatgccc cgaaaattcc
     2641 cgccacaaac aagctttcca tcaaacacgg tcaaacatcg gcctttgtca gtcaacccgg
     2701 caccgactgg tgaccagcgc tggccgttcc accgggcaat agaacgggcc gggctgttgc
     2761 ctgcggtttg aaaatcgccc gtggcat
//
LOCUS       DAOQDO010000661         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30038_length_2787_cov_2.511713, whole genome shotgun sequence.
ACCESSION   DAOQDO010000661 DAOQDO010000000
VERSION     DAOQDO010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30038_length_2787_cov_2.511713"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKZ53_11265"
     CDS             complement(<1..429)
                     /locus_tag="PKZ53_11265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41060.1"
                     /translation="MNFTFRPYRPGDEHQINQGFEAVFKTTRSLAEWQWKFSASNHGA
                     WILLAVDVQDFVVAQYAAIKRSIHFQGQFFDAGHVVDSYCRRIPQAVRQQVFPKLVLE
                     FYRYFGDAGEFQLLFGFPGDRHQKLGQLRMDYSQPVPLQCW"
     gene            complement(519..1658)
                     /locus_tag="PKZ53_11270"
     CDS             complement(519..1658)
                     /locus_tag="PKZ53_11270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ41061.1"
                     /translation="MKQSPVSCWLELPNWQKVKHMRLAVIFVHYHTPELLARSVAAIE
                     CELAALAVPSECLVVDNGSTPADRSRLQKLPVRLIEPGANLGYAGGINLGVGQTAADI
                     LIFLNPDVLVQTGCLRALLTVLDQGAAVAGPRFFWDETKRFFLPPTELRTKWAEVSKR
                     LAPFDVRLARWTRQAWRAHAQSHWLATQPFPSFTLSGALLAVRRDAWEQVGPFDTGYQ
                     LYFEETDWLARLEHKGGRACYVPDAMAVHFYNQSAAREPLALQWFAASEKRFLSCRHG
                     QFFFKTLGWLDSLPNRLHPDQDCFGQPQLDLAQLAASDDYPVWIEFSPDARGIPAAAE
                     LISTPPDKMWSFPAISWENLSPGRYFLTVVNREGLELTRLGCHRR"
     gene            complement(1558..2658)
                     /locus_tag="PKZ53_11275"
     CDS             complement(1558..2658)
                     /locus_tag="PKZ53_11275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ41062.1"
                     /translation="MLERVRLWINQTLSRPAGSPRRIAYVLDAPELCGGVKVVFQHAA
                     LLAAQGIEVVICGRGPCPDWISIPGRYVDLSKRVPRLGDFELVIATYWTTVAVAHHLK
                     CGPVAHFCQGYEGFLDHLLPQLSEIESVYRLPLPTLTVTPFLADFLKQRFGRESVLVP
                     PPVDAQFGAPLRSGPHAPARIVIPGIFECELKRVRVALDAIVQLQASGVSCVVTRLST
                     LPLSSSEAAILKPDRYLCCVPPRDVAQLLHQTDILLFPSSAGEGFGLPVLEALAAGVP
                     VIASRIPSMEFVGAQSLTLVSPDDSTGMASAAREILSNPVRWHMLAERGQLAAQRFSS
                     EAITRQLLAGIAQLAESKAHAAGRDLRSLPHA"
BASE COUNT          749 a          790 c          703 g          545 t
ORIGIN      
        1 ccagcattgc agtgggactg gctgactata gtccatccgc aattgcccca gtttttggtg
       61 acgatctccg ggaaacccaa acagtaactg aaattcaccc gcgtctccga agtagcggta
      121 aaactccagc accagctttg gaaacacctg ctgccgaacg gcctgtggga ttcgccggca
      181 ataactatca accacgtgac cagcgtcaaa gaactgtccc tgaaaatgga ttgatcgttt
      241 gatggccgca tactgagcta cgacgaaatc ctgcacatca acggccagca atatccaggc
      301 accatggttg gaagctgaaa atttccactg ccactcggcg agtgagcgcg tggttttaaa
      361 aaccgcttca aatccttgat tgatttgatg ttcgtcgcct ggacgatagg gtcgaaaagt
      421 gaagttcata gttgcaggtt agggttccgg gtctaaaagt caaaaaggac gaaaaggacg
      481 aaaaggatca acgctcgaaa acccggaagc gtggaaccct aacggcgatg acaccccaaa
      541 cgggtaagtt ctaacccttc ccgattgaca accgtcaaaa aatagcgacc aggtgacaga
      601 ttctcccaac tgatagctgg aaaagaccac atcttgtcag gtggagttga aatcaactca
      661 gcagcggctg gaatcccacg tgcatctggc gaaaattcaa tccacaccgg atagtcatct
      721 gacgctgcaa gctgggccag atcaagctgc ggctgaccga aacaatcctg atcagggtgc
      781 aaccggtttg gaagcgaatc aagccagcca agtgttttga aaaaaaactg accgtgacga
      841 cagcttagaa atcgtttttc cgatgcggca aaccattgaa gtgcaagtgg ttcacgggca
      901 gcactttggt tatagaaatg tactgccatt gcatctggaa cataacaggc ccgcccgcct
      961 ttgtgctcca ggcgcgccag ccagtcagtt tcctcaaaat agagttgata cccggtgtca
     1021 aaaggtccga cctgttccca ggcatcacgc cgaaccgcca gtaaggcccc gctcagggta
     1081 aaacttggaa acggttgtgt tgccagccag tgcgattgcg cgtgagcacg ccaggcttga
     1141 cgagtccagc gtgccagtcg cacatcaaat ggagccagtc gtttgctgac ttccgcccat
     1201 ttggttctga gttcagttgg tggcagaaag aaccgtttcg tttcatccca gaaaaaacgt
     1261 ggcccggcaa ctgccgcgcc ttgatctaaa actgtcaaca aggcacgcaa acaacctgtc
     1321 tgcaccagca catcaggatt gagaaaaatc agaatgtcgg cagcagtctg cccaacaccc
     1381 aggttgattc cgccggcata tcccaggttt gcgcctggct caatcaaccg aactggcagt
     1441 ttttgcaatc ggctgcggtc agccggcgtg ctgccgttgt ccaccaccag acattcagac
     1501 ggcacagcca gcgccgccag ttcgcattca atcgccgcca ccgaccgggc gagtaactca
     1561 ggcgtgtggt aatgaacgaa gatcacggcc agccgcatgt gctttacttt ctgccagttg
     1621 ggcaattcca gccagcaact gacgggtgat tgcttcactc gaaaaccgtt gcgcggcaag
     1681 ttgtccacgc tctgccagca tgtgccacct gaccggattg gaaagaattt ccctggcggc
     1741 gctggccata ccggttgaat cgtccggtga aaccagcgtc aacgactgtg ctccgacaaa
     1801 ctccatggag ggaatgcgcg aggcgatcac aggcacgcca gccgccaacg cttcgagaac
     1861 cggcaggcca aacccttcac ctgcggatga aggaaacaaa agtatatccg tctggtgcag
     1921 aagttgtgca acgtccctgg gcggcacaca acacagatag cgatccggct tgagaatggc
     1981 cgcttcagaa ctggaaagtg gcagggttga aagtcgagtc accacacacg aaaccccact
     2041 ggcctgcaat tgaacaatgg cgtcaagcgc cactcgaacc cgttttaact cgcattcaaa
     2101 aatccctgga atcacaatcc gggccggagc atgcgggcca ctccggagcg gtgccccaaa
     2161 ttgcgcatca actggcggcg gcaccaggac cgactcacgt ccaaaccgct gcttgagaaa
     2221 atcagccaga aatggtgtca cggtcaaagt tggtagtggc aaccgataaa ccgattcaat
     2281 ctcagagagt tgcggtaaaa gatgatcaag aaatccttca tatccctgac agaaatgagc
     2341 caccgggccg cacttgaggt ggtgggcaac cgcaacggta gtccagtagg tggcaattac
     2401 cagttcaaaa tcacccaacc ggggaacacg ttttgaaaga tcaacatagc ggcctggaat
     2461 gctgatccag tcagggcagg ggccacgtcc acaaatcacc acctcaattc cctgtgccgc
     2521 aagcaacgcc gcgtgttgaa acacaacctt gacaccgcca cataattcag gcgcgtccag
     2581 cacataggca attcttcgcg gtgaaccagc cggtcgggat agagtttgat tgatccacaa
     2641 acgaacgcgc tccagcatct ctttccttac cttctggtta caggctgtaa ccaacctgcc
     2701 tcaattcaac cacggctccg cagtgaaatc cacagccagg agtattcgaa aaccctgaac
     2761 cctgaaccct gaaccctgaa ccctgaa
//
LOCUS       DAOQDO010000662         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30160_length_2780_cov_3.642936, whole genome shotgun sequence.
ACCESSION   DAOQDO010000662 DAOQDO010000000
VERSION     DAOQDO010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30160_length_2780_cov_3.642936"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..467)
                     /locus_tag="PKZ53_11280"
     CDS             complement(66..467)
                     /locus_tag="PKZ53_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ41063.1"
                     /translation="MPEPGKEMIKAAKTGNLLKIKDLLATDTTLVAARDSDGSTPLHC
                     ATWKGHLEIVIWLLEAGADVNAHNQNDHWGTTPLHAAAHANQAAIAQVLLDHGADVNA
                     QDLNGKTPLFHTTIHKAKAVEKLLQKHIGKV"
     gene            complement(569..955)
                     /locus_tag="PKZ53_11285"
     CDS             complement(569..955)
                     /locus_tag="PKZ53_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41064.1"
                     /translation="MKIPKAIRILLAAIAVTLLGAGVFWHDNYGPTAFDPVAWRQGEQ
                     AEFSPDALRLRMADGLVQSKVLVGKPKAEVLAMLGAPSQTDKFKEYDLVYWLGAERGF
                     ISIDSEWMVLQCTETGIVKEACIVRD"
     gene            complement(1081..1773)
                     /locus_tag="PKZ53_11290"
     CDS             complement(1081..1773)
                     /locus_tag="PKZ53_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41065.1"
                     /translation="MKNLLLMTGFFLWTQLALAQEFPSPSDFEIEGSTKQVQNVTLTT
                     AIIDLDQNGIDDALTLQLSSGAEVVDEAPCAGCGHRFEGKFVVLVQMNGAKTSRTQLN
                     QFFQRSELSFWAKPWSISFADYNHDGILDFNLGQFENSNNWEYRLFTVTSGGKVKSLS
                     IPDGAIYVCDDSNSTSRIFQTQAGFWYKAFSNGEEAGWHQFFLNWNTRHKQFELVQHR
                     HSMDEPESPASY"
     gene            complement(1948..2541)
                     /locus_tag="PKZ53_11295"
     CDS             complement(1948..2541)
                     /locus_tag="PKZ53_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41066.1"
                     /translation="MIGKLSTVIARWYSTPVMICFLVLAFCLVWVFNFSSLPISNSEL
                     IKLSGGPGLLDLRFYYSGQEATTTLASYGDAGRAFYRNYLIFDFFFPVMYGFGLAWML
                     TLIARKAFGDNSRWMQVNLLPVGIASADYVENLCILWMLTSFPDNNIWTGTLAGMATV
                     TKHILAIGTLRCMAVGSGALVARRYGFFQRWLKPQNS"
     gene            complement(2565..>2780)
                     /locus_tag="PKZ53_11300"
     CDS             complement(2565..>2780)
                     /locus_tag="PKZ53_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41067.1"
                     /translation="VPALQKNANYFDTEILEYYWISGCKKNGQDALYSTTIEVDDDVR
                     EEYWTHIRNRPEMKNVRTIRSTGKYSR"
BASE COUNT          704 a          647 c          683 g          746 t
ORIGIN      
        1 ggcaatgatt ttatttcctt tggcttgcgg agaggtcgca tcaccctgga gatatgtgat
       61 agcgatcata cttttccgat gtgtttctgc agcaactttt ccacggcttt ggctttatgg
      121 atggtggtgt gaaacaaagg tgttttccca tttaaatcct gagcattcac atcggctccg
      181 tggtcgagta acacctgggc aatcgcagct tgattggcgt gggctgccgc atggagcggg
      241 gttgttcccc agtggtcatt ttgattatga gcgttgacgt ctgcgccagc ttcgagaagc
      301 cagatcacta tttccaggtg tcctttccag gtggcacagt gcagaggagt tgaaccatca
      361 ctgtcgcgag ccgcaacgag tgtggtatca gtcgccaaaa ggtcttttat tttcagcaga
      421 ttacctgtct ttgctgcttt gatcatttct ttacctggtt cgggcatact tgactccttt
      481 gcaaactcag aatttttcaa ttgaatgagt ttgtataact gagttatgtg aggtggtcaa
      541 gatagttcaa ttgaggacgg tggaatcttt agtcccggac aatgcaggct tctttcacaa
      601 tgccagtctc tgtgcattga aggaccatcc attcgctgtc aatactgatg aagcctcgct
      661 ccgcgccgag ccagtagacg agatcatact ctttgaattt gtctgtctga gaaggcgcac
      721 cgagcattgc caggacctcg gcttttggtt tcccgaccag tacttttgat tgaaccagtc
      781 catccgccat ccgcagtcgc agagcatctg gtgaaaactc agcctgctcg ccttgtcgcc
      841 aggcaactgg atcaaaagca gttgggccat agttgtcgtg ccaaaaaaca cctgcaccaa
      901 gcaaagttac tgcgattgca gccagaagga tgcgaatagc ttttggtatt ttcatgggtg
      961 tgacctctgg gaacaaatca actcagaagt aactggaaag tcaaaggttc tgcggtttga
     1021 gccagggttg aaagaacagc cagaagttgg ttgcccgtct ggtatttctt ttgaagcccc
     1081 ttaataactg gctgggcttt caggttcatc catcgagtga cggtgttgta ccagctcaaa
     1141 ttgtttgtgc cgggtgttcc agttaagaaa aaattgatgc cagccggctt cctctccatt
     1201 tgaaaaagcc ttgtaccaaa agccagcttg agtttgaaaa atgcggctgg tggaattgga
     1261 atcatcacaa acgtaaatcg cgccatctgg aatggataac gattttactt ttccgcccga
     1321 tgtaactgtg aagagtcggt actcccagtt attgctattc tcaaactggc caagattaaa
     1381 atccagaatc ccatcgtgat tgtaatcagc aaaagaaata gaccacggct ttgcccaaaa
     1441 tgacaattcc gaccgctgga agaactgatt gagttgggtc ctgcttgttt ttgctccgtt
     1501 catctgaacc aggactacaa attttccttc gaagcgatgc ccacagccgg cgcaaggagc
     1561 ttcatcaaca acttcagccc ctgaggaaag ttgcaaggtg agtgcatcgt caatgccatt
     1621 ttggtcgaga tcaatgatcg ccgttgtcag tgtcacattc tggacctgtt tggtactgcc
     1681 ttcgatctca aagtcggatg gtgatggaaa ctcttgagcc agagcaagtt gagtccacaa
     1741 aaagaaacca gtcatgagca ataggttttt cattgtattt tcctcatgag gtgaaataag
     1801 aagggataga aaagatatac aatgaaggcg cgatcttcag gcgtatcaca cacactgatt
     1861 caacggtaat caccacattt atttcaagct aaatcacctc aatgagggat aggcttccaa
     1921 tgcgtggggt cttttgttgt ttcttgatca ggaattttgc ggtttgagcc accgctgaaa
     1981 aaatccatac cggcgagcga ccagtgctcc gctcccgacg gccatacacc gcaaggtgcc
     2041 aatggcgagg atgtgtttgg tcactgttgc cattcccgcc agtgttccag tccagatgtt
     2101 attgtccgga aatgacgtca acatccacag gatacacagg ttttccacgt agtcagcgct
     2161 ggcaattccc actggcagga ggttgacctg catccagcgc gagttgtcgc caaaggcttt
     2221 gcgggcaatc agcgtcagca tccacgccaa accgaagcca tacatcactg gaaagaaaaa
     2281 atcgaaaatc aggtagtttc gatagaatgc gcggcccgca tccccatagc tggccagtgt
     2341 ggtggttgct tcttgaccag aataataaaa tctcaggtcc agcaaccctg ggccaccact
     2401 gagcttgatc aactcggaat ttgagatggg gagacttgaa aagttaaaaa cccaaaccag
     2461 acagaacgcc aggaccagaa agcaaatcat aactggtgtt gagtaccagc gagcaataac
     2521 ggtgctcaat tttccaatca tcggattctc cctgggtaaa gcttttatct ggagtacttg
     2581 ccggtagatc gaatggtccg gacgtttttc atttcggggc ggttacggat gtgagtccag
     2641 tactcctcgc ggacatcgtc gtcaacttca atcgtggttg agtacagtgc atcttgtcca
     2701 ttctttttgc aaccagagat ccagtagtat tcgaggattt ctgtgtcgaa ataattagca
     2761 ttcttttgga gtgcggggac
//
LOCUS       DAOQDO010000663         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30234_length_2776_cov_3.377435, whole genome shotgun sequence.
ACCESSION   DAOQDO010000663 DAOQDO010000000
VERSION     DAOQDO010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30234_length_2776_cov_3.377435"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKZ53_11305"
     CDS             <1..181
                     /locus_tag="PKZ53_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbitol dehydrogenase"
                     /protein_id="HNJ41068.1"
                     /translation="VSSYSCGPDDTQEALNLIESGVITAEKLVTHRFPLHETTQAFRK
                     MAEARDVLKALVVFP"
     gene            complement(351..1988)
                     /gene="ccsA"
                     /locus_tag="PKZ53_11310"
     CDS             complement(351..1988)
                     /gene="ccsA"
                     /locus_tag="PKZ53_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNJ41069.1"
                     /translation="MSTNPVNLEVPFSAASVKPHAGLTSSKSFTYIIPLILALGLVML
                     RWNNIAPNILKEGNLTVVALIAYLSASVVFVSYLLGLGDKQLLQRIGLWTMAFGFVLN
                     FAGWGIRWIEIVDHFKQQPAIGPIWASMPWTDKVSHTYPLTGLYDVGIAFTSIAVFSS
                     LVIASRKKYDFIGFVTMPISALVMILCVFLGNEISTLQPILRSYWRPIHVSLAAISYG
                     VCLVSFGVAVLHLLKEKVKIESMGFWVAVLGILTYIIIGDFKVLTTGSYGLGVRAMGS
                     GLNLAGGGNLRAELPGVGHLMQLGTLVYLAAAICLALFVLQAKEQMQIWGNRLMMAAL
                     VIQVLTFGALYTQMKAITGSVMSHINQSQFQAFGAWLVKNSDQDPTQFAPLQLVGFAQ
                     DFLNTQSATLSVAFASNPIEMAAILTLIACTGFIVLFVWKGQTLMASLPSTEALDDLT
                     YKTVSVCFPMLAMMLITGAVWANESWGTYWSWDPKETWALITWLAYAGYLHARIVHGW
                     KGKSSAYFAVIGFLFVIFTYLGVSFVLPGLHSYAGVD"
     gene            2387..>2776
                     /locus_tag="PKZ53_11315"
     CDS             2387..>2776
                     /locus_tag="PKZ53_11315"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41070.1"
                     /translation="MTPTEFVASIQGGKIEPVYLVMGPETYLQSECLRLLTRQLIPDP
                     EAQQFGYSEHSVAERSLGTVLGIAGQLPMMSSAQVVVAREFDKVSDAEIEALKNYLRR
                     PNPSTVLVFQTTDLDKRRTLSTILLKGC"
BASE COUNT          732 a          707 c          697 g          640 t
ORIGIN      
        1 ggtgtcgagc tattcgtgcg ggccggatga tacccaggaa gccctgaacc tgattgaatc
       61 tggtgtaatt acggctgaaa aactggtgac ccaccggttt ccgcttcacg aaaccaccca
      121 ggcgtttcga aaaatggccg aagccaggga tgttctcaag gcgctggtag tttttcctta
      181 ggaagagggc tgaagaaggt gggctgaaga aaatgggctt ggggctgaaa acttcgggct
      241 gaagacatcg gggtgagatt gcacattgac ccgttttctt cagcccaact ttcttcagcc
      301 ccgagcttgc gagtcttcag ccccaagccc attttcttca accctgtttt ttagtctaca
      361 ccggcgtatg agtgcaggcc cggaagaaca aaactcacgc caagataggt aaagatcaca
      421 aataaaaagc cgataaccgc aaagtacgcc gagcttttac ctttccatcc gtgaacaatc
      481 cgggcgtgca ggtatccggc ataggccagc caggtaatca gtgcccaggt ctctttcggg
      541 tcccagctcc agtatgtgcc ccacgattca ttggcccaga cggcgccggt aatcaacatc
      601 atggcgagca ttgggaagca aaccgaaacc gttttataag tcaaatcatc aagggcttcg
      661 gttgaaggca aagatgccat cagtgtttgc cctttccaga caaataaaac gataaatccg
      721 gtgcaggcaa tgagggtcaa gatagcagcc atttcaatcg gattggaggc aaatgcaacc
      781 gataatgtcg ccgactgagt attgaggaag tcctgggcaa agccaaccag ttgtaacggc
      841 gcaaactggg tcggatcctg gtctgaattt ttcaccagcc aggcgccaaa tgcctggaac
      901 tggctttgat tgatatgact catcacgctg ccagtaatgg ctttcatctg ggtatagagt
      961 gctccaaatg tcagtacctg aatcaccagg gccgccatca tcaggcggtt tccccagatt
     1021 tgcatttgct ctttggcctg gagtacaaac agcgcgagac aaatcgcggc agccagatag
     1081 accagcgttc ccagttgcat caggtgcccc acccccggca actcagcccg cagattccca
     1141 ccgccggcaa ggtttaatcc gcttcccatc gcccgaaccc cgagaccata ggacccggtc
     1201 gtcaatactt taaaatcgcc aataatgata taggtcagga tgccgagtac tgccacccaa
     1261 aaccccatgc tttcgatttt gactttttct ttgagcagat gcaggacggc aacgccaaaa
     1321 ctcaccaggc agactccata gctgatggct gccagactga cgtggattgg acgccagtag
     1381 ctgcgcaaaa tcggctgaag cgttgaaatt tcattgccca gaaacacgca caaaatcatg
     1441 accagcgctg aaatcggcat ggtcacaaag ccgatgaagt catacttttt ccggctggca
     1501 atcaccagac ttgagaagac cgcgatactt gtaaacgcga taccaacgtc atagagcccg
     1561 gtcaatggat aggtatgcga gactttatcg gtccacggca tggaagccca aatcggacca
     1621 atcgccggct gttgtttgaa atggtcaaca atttcaatcc agcgaattcc ccatccggca
     1681 aaattgagta caaacccaaa ggccatggtc cagaggccaa tgcgctgcaa aagctgttta
     1741 tcccccagac caagcaggta actcacaaac accacgctgg cgctcaggta ggcaatcaag
     1801 gccaccaccg tcagattgcc ttctttcaaa atgttgggag cgatgttatt ccagcgcaac
     1861 atcacaagcc cgagggccag gatcagcggt atgatgtagg taaaactttt gctcgaagtc
     1921 aggccggcgt gtggctttac gctggcagcc gaaaatggga cttcaagatt gacagggttg
     1981 gttgacataa caaaaacctc ttgcagagtg atcttatggc ggaaagagcc gaatcaggtt
     2041 ggagaacaaa attgatttgg gtgttcttgg ggtgaagcct cacgagtata ggcgaaccag
     2101 acggacaacg gcaaggattc cacgacgttc gattgcacct cagcgcccat tggtggcgaa
     2161 tgaatccggt ctttttgaat gcctgagctg gatttttgtt ggattacaac ttctgtgaaa
     2221 aagacgggaa acctttcaac ctgaatcaaa ttcttcgggt tctctgaatt ttggcaggtg
     2281 caaaaggtgg tcaggccgtt caaatcagtg ttactctccg ccttcaattc caggatcaat
     2341 tgatcttcac gcagtcggaa ctcactccat aagtcttgct cttcatatga cgccaactga
     2401 gtttgttgct tcaattcaag gtgggaaaat cgaaccagtc tatctggtga tgggaccgga
     2461 aacctatctg cagtcagaat gtttacgtct gttgacccga cagctcattc ctgatccaga
     2521 ggcacagcaa tttggctatt ctgagcattc ggttgctgaa cggtcacttg gaacggtgct
     2581 ggggattgcc gggcagttac caatgatgtc atcagcccag gtggtggtgg ctcgtgaatt
     2641 tgacaaagtg tcagatgctg aaattgaagc gctcaaaaac tatttacgcc ggccaaatcc
     2701 ttcgacggtt ctggtgtttc aaaccacgga tctcgataag cgccgcacgc tgtctaccat
     2761 tctgctcaaa ggttgt
//
LOCUS       DAOQDO010000664         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30314_length_2772_cov_3.813029, whole genome shotgun sequence.
ACCESSION   DAOQDO010000664 DAOQDO010000000
VERSION     DAOQDO010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30314_length_2772_cov_3.813029"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1228)
                     /locus_tag="PKZ53_11320"
                     /pseudo
     CDS             complement(83..1228)
                     /locus_tag="PKZ53_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            1910..>2772
                     /locus_tag="PKZ53_11325"
     CDS             1910..>2772
                     /locus_tag="PKZ53_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNJ41071.1"
                     /translation="MDLSQLEVFLAVAQEKSFSRAAQKLYRTQAAVSQSIRKLEEDVG
                     EVLFDRAFKDGRLTDAGELLFDFAQEMLNVRREATNALKELRSLHQGKLQIGANEYTV
                     MYLLPVLAEFRRLYPRIKVEVKRSRASEIPMQIIHREVDMGVISFPPQEPGLQSIEVG
                     TDSLVLVVGPTHPLASKDQVSITELEMESFAAHNVVSPYRQKVMETFQRYKTPLNITL
                     ELPSIEAIKRFIQFGSGVCILPKLAVQNELDHKLLVGIPIKEMKVERKLYLVHRRKSG
                     LSHAGKAFLRLA"
BASE COUNT          683 a          686 c          736 g          667 t
ORIGIN      
        1 acccggaacc cggaacccgg aacccggaac ccggaacccg gaacccggtt cttcaagctg
       61 tcatccgtga aatttcgtgg acatcttcag ccagagaaat caggtcgctg tcagtgattt
      121 ctttcttctg gtcagccagc gaagtgaagt gacgatagag ccggtcaatt tcaaaccgat
      181 taaaccgata gccgagccgt tcaaaatgtt taatcaaagc gtgacgcccg gaatgtttcc
      241 ccagcaccag ccggttttca ggaacaccaa ctgattcggg tgtcataatt tcgtaggtca
      301 gccgattttt gagcacgccg tcctggtgga tgcccgcttc gtgggcaaac gcattgcgtc
      361 cgacaatggc cttgtttggc tgaacgggaa tgtttgtaat ttcagacaaa agctgactgg
      421 ttggaaataa caattccgat gcaatttggg tttgataggc cagcacgtcg cgtcgcacgg
      481 aaagagccat tacgagttct tcgagcgagg cattgccggc gcgttcgcca atgccgttga
      541 tggtgcattc gacctgacgg gcaccagctt cgatggcggc cagtgaattg gccaccgcca
      601 gccctaaatc gttgtgacaa tggacgctga tcgccgcctg atggatgttg tccacgcgct
      661 caaacagcgt tttgatgatc actgacattt cagagggaat cgtgtagccc accgtgtcgg
      721 gaatgttcac gaccgttgcc ccggcggcga tcacttcgcg cacaacctgg cacagatatt
      781 caagatcagt gcgggtggcg tcttcaggtg aaaattcaac gtcatcggca aaggtgcgcg
      841 ccagttttac ggcctgacag gcttgatcga gaacctgttc ccggctgcgt cgcaatttgt
      901 aagtcagatg aatgtcggat gtggcaatga aggtgtgaat gcggggatga tgggcaggag
      961 ccagtgcttt tccagcgcag gtaatgtctt cgtcgcaggc ccgggccagt gcggcaatcg
     1021 ttggccgacg gaagttgata gatacagcgt tgacagcagc ccaatcgcct tcggatgctg
     1081 ccggaaagcc ggcttcgata acgtctacac tcagacgatc aagctgatga gccatccgca
     1141 gcttttcgtt gatcgtcata ctacaccccg gagattgctc tccgtcgcgc agcgtcgtgt
     1201 cgaagactat gacacgtgcg gtatccattg cacgaactcc ttttctatca agatgataca
     1261 gggtaaacct gaagaaatcc gcttcgctgg caaaacacac ctctcccagg tctgggcaca
     1321 cggtgccctg acgagctcag acgggtgcga atgaagtgga tttcctggaa aaatgccacg
     1381 cggcagattg cctgacctga cgcgtttgaa gaaatggtaa cgataccgat gaattatgag
     1441 aaagttatct cgctgcaatg agatgcactt cgatgttcat aattcagata tatctagcca
     1501 gggcgaaggt tgggctcaca ttccccctct acctacgtac cccaacctgg cccagtctag
     1561 ttattgagtc agccaatgcc aaaggcaggg ctttggaaag cctttccctg ttgggaattg
     1621 cagcccttca acagatgaag ggcggaatga cttattggcg agggctacgg tagaggaaaa
     1681 aaaaactata gtcaaattta taattcttgt aatatcataa gaattgctta tgatataagt
     1741 gtaattgaat tacacttcag tttttgctaa tactgatgag cattgttttt gtggttttga
     1801 gcacgtgtga gcgtgacttc aacgacttgc aaaatccttt tctcttcaga gttttacaag
     1861 caccagtgac cgagttgaaa caggacgttg cggaggcacc aaccagggta tggatctttc
     1921 tcaattagag gtttttttag ccgtcgctca ggagaaaagt ttttcccgag cggcccaaaa
     1981 actgtaccgg acgcaggcgg cggtcagtca gtccattcgt aaattggaag aggatgttgg
     2041 cgaagtcctg tttgaccgtg catttaaaga cggtcgcctg acagacgccg gcgagttgct
     2101 ctttgatttt gcccaggaaa tgctcaatgt tcggcgcgag gccaccaatg cgctcaagga
     2161 actccgcagc ctgcatcagg gaaagctcca aatcggggcc aatgaataca ccgtgatgta
     2221 tttgcttccg gtgttggctg aatttcggcg gttatatccg cgtatcaagg ttgaggtcaa
     2281 acgcagccgg gcgagtgaga tcccaatgca aatcattcat cgtgaagtag atatgggggt
     2341 catctcgttt ccgccgcaag agcccggatt gcaatcaatc gaagttggaa ctgattcact
     2401 ggtgctggtc gttgggccaa cgcatccgct ggcttcaaaa gaccaggttt caatcaccga
     2461 actggaaatg gagtcatttg ccgcgcacaa cgtagtctct ccgtatcgtc agaaagtgat
     2521 ggaaacattc caacgctaca aaacgccgct caacatcacg cttgaacttc cctccattga
     2581 agcgatcaaa cgctttatcc agtttggcag cggcgtctgt atcttgccga aactggccgt
     2641 tcagaatgaa cttgaccaca agctgctggt gggtattccg atcaaagaaa tgaaagttga
     2701 gcgcaaactg tatctggtgc atcgccgcaa gtcaggactt tcgcatgccg gcaaggcatt
     2761 tctgcggctg gc
//
LOCUS       DAOQDO010000665         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30397_length_2769_cov_2.058585, whole genome shotgun sequence.
ACCESSION   DAOQDO010000665 DAOQDO010000000
VERSION     DAOQDO010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30397_length_2769_cov_2.058585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2769)
                     /locus_tag="PKZ53_11330"
     CDS             complement(<1..>2769)
                     /locus_tag="PKZ53_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ41072.1"
                     /translation="RLSAVFRPGTLEVTIPYLTSQKLGRGTLNVEILGPDNKVIASHS
                     ESDQLNAGEQGVWKAKLAIPATLQREDIVWHRLQYRFSSPQSTADAEPAGIHAISSIL
                     RFPVVRVLGQQQYGVGSPSAVRLVAVDGFDGSPLTQGTLELALKTEKTEFQLGKADLD
                     THGTVNLPLNFPAETVGKASLKINVDTPVGTQELVQPITLVRRESILITTDKPMYQPG
                     QMIHLRALALDRFTRAAANNRQSIIEIEDAKGNKVFKRRTQTDAFGVAFADFQLADEV
                     NMGAYKLRVILGAENDPNSVTQERTVTVDRYVLPKFKIEVNFDGDAQGQKRRYYAPGE
                     TVAGTITAKYLFGKPVSNGKVTLKLSTFDVSAVELGNLELQTDADGKAHFSSQLPSSF
                     AGSSFTQGSAPVACEISVTDTANHTESKSEQLLVSSNPILILAVPESGKLVPNLENKV
                     YILTSSPDGVPVSATVTAKGLTPGVIQTDANGFAVATVSAKAQAVELALEARDAGGKT
                     GKATLSLSPETLTEDSLILRTDRTTYKVGETVAITGISTKQKGSLYLDIIKDRQTVLT
                     TALEFENGKVEFPLDLSANLFGTLELRAYTFGRNADPISDRKLIFVDPADDLKVAVST
                     PKESFKPGESAVLNFTVTDAAGRPKRAVIGVEIVDEAVFALSEKQPGFEKIFFYLEQE
                     LLKPRYEIHSFSSDEIVPASLTEPVQMVRRETAAKVLLAAATEINPYTVKTDAGRDFL
                     SAKQNEYASSYYQINYAQSTKLVEGLNRYFAEQHITRFELPGDLPKAVKARYVKQSDL
                     LDPWGKPYQFGNQYSYNYGAQRYSYGYVQSFGHDGVQGGPTDFSLLYYLTRPASELKT
                     GDGFNGKVKIETNANLRNGESFVVGKVTEPNSNSIAQASVIISRLNNKGQAKQIIRSM
                     ATDSSGTFT"
BASE COUNT          595 a          684 c          771 g          719 t
ORIGIN      
        1 ggtgaatgtt ccacttgaat cagtggccat tgagcggatg atttgtttgg cctggccttt
       61 gttgttgagc cgggaaatga tgaccgaagc ctgggcaata ctgttgctat tgggttcggt
      121 aactttgcca accacaaacg attcgccatt gcgcaaattg gcattggtct caattttgac
      181 tttgccgtta aacccgtcac cggttttgag ttctgacgct ggacgagtga gataatatag
      241 cagggaaaaa tcggttggcc caccctgtac tccatcgtgg ccaaagctct ggacatagcc
      301 gtaactgtaa cgttgcgcgc cgtaattgta tgaatattga tttccaaact ggtatggctt
      361 gccccacggg tcgagtaaat cgctttgctt cacgtaccgg gctttgacag ctttcggtaa
      421 atcgccgggg agttcaaacc gggtgatatg ttgttcggca aagtaccggt tgagcccttc
      481 aaccagtttt gtggactgcg catagttgat ctgatagtaa cttgacgcat attcgttttg
      541 tttggccgac aggaaatcac gcccggcatc ggttttgacc gtgtacggat tgatttcggt
      601 cgcagcggcc agcagcactt tggccgctgt ttcgcgccgc accatctgga caggttcggt
      661 caggctggct ggaacaattt catcgctcga aaagctatgg atttcatagc gcggtttgag
      721 cagttcctgt tcgagataga agaaaatctt ctcaaaaccg ggctgttttt ccgagagcgc
      781 aaacacggct tcgtccacga tttcaacacc aatgaccgcc cgtttcggac gaccagcggc
      841 atcggtcacg gtgaagttga gcacggcgct ttcgccgggt ttaaagcttt cttttggtgt
      901 cgaaaccgct actttcaggt catcagccgg atctacgaaa atcagcttcc ggtcactgat
      961 cgggtcagca ttacggccaa aggtgtacgc ccgtaactcc agcgttccaa ataaattggc
     1021 tgacaaatca agtggaaatt ccactttgcc attttcaaat tcaagtgccg tggtgagcac
     1081 tgtctggcgg tctttgataa tgtcgagata gagcgagcct ttttgtttgg tggaaattcc
     1141 ggtaatcgcc acggtttcac cgactttgta ggtcgtgcgg tccgtgcgca aaatcaggct
     1201 atcttcggtc agcgtttcag gtgacagcga aagcgtggct ttcccggttt tgccgccggc
     1261 gtcacgggct tccagtgcaa gttcaacggc ctgggctttg gccgaaacgg tggccacggc
     1321 aaacccattg gcgtcggtct gaatgacacc cggtgtgagt cctttggccg tcaccgtggc
     1381 tgaaaccggc acgccatcgg gggaagacgt caaaatgtag accttgtttt ccagatttgg
     1441 caccagtttg ccgctttccg ggacggccag tatcaaaatc ggattggacg aaaccagtaa
     1501 ctgttcgctt tttgattcgg tgtgattggc ggtgtcggtg accgaaattt cacaggccac
     1561 gggggcgctc ccctgggtaa aactgcttcc ggcaaaactg gaaggtagct ggctgctgaa
     1621 atgggctttc ccgtcggcat cggtctggag ttcaaggttg ccaagttcca ccgccgacac
     1681 gtcaaacgtg gaaagtttga gtgttacttt gccatttgaa accggtttgc caaacaaata
     1741 tttggcagtg attgtaccag caaccgtctc gccgggagca taatagcgcc gtttctgacc
     1801 ctgggcatcg ccatcaaagt tgacttcgat tttaaatttg ggcagcacat agcgatcaac
     1861 cgtgacggtt cgttcctggg tgacgctgtt cgggtcgttt tccgcaccca aaatcacccg
     1921 gagtttatac gcccccatat tgacttcatc cgccagttga aaatcggcaa aggcgacacc
     1981 aaaggcatca gtttgcgtgc ggcgtttgaa gactttgttg cctttggcgt cttcaatttc
     2041 aatgattgac tgacgattgt tggcagcggc acgagtaaac cggtcaagcg ccagggcgcg
     2101 gaggtggatc atctggccgg gctgatacat cggcttatcg gttgtaatca gaatgctttc
     2161 gcggcgaacg agcgtgattg gttgcacgag ttcttgcgta ccaaccggtg tatctacgtt
     2221 aattttcaga ctggctttgc cgacggtctc ggctgggaaa ttgagcggca gattcactgt
     2281 tccgtgggtg tcgagatcgg ctttgccaag ctgaaattcg gttttttcag ttttgagcgc
     2341 cagttccaac gtgccctggg tcagcggcga gccatcgaac ccgtcaacgg ccaccagtcg
     2401 gacagcggag gggctcccaa cgccatactg ttgttgaccc agtaccctga ccaccgggaa
     2461 gcgcaaaatg gatgaaatcg catgaattcc agcaggttcg gcatcagcgg tagactgagg
     2521 acttgaaaac cgatactgga ggcgatgcca gacgatgtct tcccgctgga gcgtggctgg
     2581 aattgcgagt ttggctttcc agacaccctg ttcaccggcg ttcaattgat cgctttcgga
     2641 atgactggca atcactttgt tgtctgggcc gagaatttct acgttgagtg tgccacgtcc
     2701 aagtttttga ctggtcaggt aggggatggt gacttcgagt gttcccggtc gaaaaacggc
     2761 tgacagcct
//
LOCUS       DAOQDO010000666         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30428_length_2767_cov_3.386799, whole genome shotgun sequence.
ACCESSION   DAOQDO010000666 DAOQDO010000000
VERSION     DAOQDO010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30428_length_2767_cov_3.386799"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            690..>2767
                     /locus_tag="PKZ53_11335"
     CDS             690..>2767
                     /locus_tag="PKZ53_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ41073.1"
                     /translation="MRSSWAAPRQSNPLTLKISQRRPVVNEGNQITLTATDDQGNPLS
                     EVQWSSGSPDIAAVDPVTGKVQGRQRGFATITAFQNGHSASVFVTVVRVEKRKGEKVR
                     GETRLDGKGQIYLSNPLQNVIMRADSALNASFEVFAGSKNVPGIRDGLRYQSLFAGPT
                     AIGIDSSTTGGVYIADTLNHSLRKITFTNEVQTIVGAGAPGIPRFDSTGALPLSQLRL
                     SSPRGVAVEAGGNLFVADTDNHSIYYVDFATHQARLVAGNPGESGKVDGPGADARFFR
                     PAGLTLSSDGRTLGVADEGNNRIRLIELARASDGSFSGQVTTIGVASSTRGVAIQSLR
                     QMDNPRDEIVFRQPSSLSFDTVGNLNVVDQSGVYVILRPLGKLPEVVELAQPEVTFMK
                     PVSVTVNQAGSYVLDQNALEEESLKVVAVGAPRILSLSRETARIDGGTEITITGENFA
                     PESIVTLGSTAIQEAEVVTATEIRFRVPPQFLPGERALSVQTRGGISQAKLIILPKQL
                     EEMSPGESSFIVGGTPYNGDGAIATDPSVVLETQLFTVDTLGNIYTADGSRTRRIDAG
                     TGIVTTIIGGDIAGYSDDGDLAQTAKLSDAHEVVLDRAGNLFIADNGGRRIRRVDALT
                     GVITTVVGGGRLRSDNAPADKFLLVKAQGLTIDHNNRLYFEDLNQIFSLDPETQTVHI
                     VAGTGQRGASG"
BASE COUNT          672 a          648 c          754 g          693 t
ORIGIN      
        1 gacccaccac cgttcctgtg acggtttgag ccctgaccat tgtgccgcca gtcagatggt
       61 gaaaatacag aatggtactg gtaagtccaa taaataaaaa gagttgaaaa agaaagcctt
      121 tgattgtttc tgacatgatt tgcaaaaaaa tgaagacacg tcgaatgagt tggaatacac
      181 cggttggatc ataaatggcg atgaaccaaa gttcaccgat aacgcgtgaa aaccagacga
      241 acattcgcac acattgaaaa ataaatgagg aatcgttgaa ccaggcgagg ccttactgaa
      301 aaagaatcta ttgacacggc gctggccgta tccgccatcc gttctcaacg accgtcattt
      361 gattcttgct caggcgatga cttcattctc tcgtttccat ctcttgaaaa actatcttcc
      421 gtgagaatga atctccattt tttacgcgtt ctaaggcaaa caagccagca ttgtattttt
      481 ggaacactgg ctcccaatct ttttcaccca atgtggttcg caccagttat ctcaatgatt
      541 gacgcttttg gtcgtcaatc gctctaaatt tttccaagaa gcgaagtgta atctgtatgt
      601 accgtcgaat cataccttta gggttgatat cagtcatagc tctatgcctg gggtgtctgg
      661 cgccaccatc ggtatggatg aattctgtct tgagatcatc ctgggcggca cctcgtcagt
      721 ccaatccatt gacgctcaaa attagccagc ggcggccagt ggtgaacgaa ggaaaccaga
      781 ttacactcac ggccacagac gaccagggga accctctgtc ggaagtacag tggagttcag
      841 ggagtcctga tattgctgcg gtggatccgg tgacgggaaa agtgcagggg agacaacgcg
      901 gatttgccac cattaccgct tttcaaaatg ggcattccgc ttcagttttt gtcacggtgg
      961 tgcgggttga gaaaagaaag ggagaaaagg tccgtgggga aacccgttta gatggcaagg
     1021 ggcaaatcta tctgagtaac ccattacaaa atgtgattat gcgggccgat agcgcgctca
     1081 atgcttcatt tgaggtcttt gccgggagta agaatgtccc tggtattcgg gacggtttgc
     1141 gataccagtc gctttttgct ggacccacgg ccattggaat tgatagcagt accactggcg
     1201 gggtgtatat tgccgataca ctgaaccata gtttgcgcaa aatcacattt accaatgagg
     1261 ttcaaacgat tgtcggtgct ggtgcacctg gaatccccag gtttgactca actggcgctc
     1321 tgccgctctc tcagttgcgg ttgtcgagcc cgcgcggcgt ggctgttgaa gccggaggca
     1381 acctgtttgt cgctgatact gacaaccaca gcatttatta tgtggatttt gcaacgcatc
     1441 aggcacgact cgtcgctgga aaccctggtg aatcaggaaa agtggatgga ccaggggccg
     1501 atgctcgctt tttccgtccg gctggcttaa cgctgagtag tgatggtcgt actctgggtg
     1561 tggctgatga aggaaataac cggattcgcc tgatcgaact cgcccgagcg agtgatggaa
     1621 gtttctcggg tcaggtaacc acgattgggg ttgcgtcgag tacgcgtggt gtcgcgattc
     1681 aatccttacg ccagatggat aacccccgtg atgaaatcgt atttcgtcag ccgagcagcc
     1741 tgagctttga cactgtgggc aatctgaacg tcgttgatca atccggggtg tatgtgattt
     1801 tgcgtccgtt gggcaaattg ccggaagtgg ttgaactggc tcaacctgaa gtcactttta
     1861 tgaaaccggt gagcgtcacc gtcaatcagg ctggaagcta tgtgcttgac cagaacgccc
     1921 tggaggaaga atcactcaaa gttgtggccg tgggtgcccc tcggattctg tcgctgagtc
     1981 gggagactgc ccgcattgat ggcggcaccg aaatcaccat cactggtgaa aattttgcgc
     2041 cggaaagtat tgtcacgctt gggagcaccg cgattcagga agccgaagtg gtgacggcaa
     2101 ccgagattcg atttcgggtc ccgcctcaat ttttacctgg tgaacgggcc ctgagtgtgc
     2161 aaactcgcgg agggatttcg caggccaaat tgatcattct tcccaaacag cttgaggaaa
     2221 tgtcacctgg ggaatcaagt tttatcgtgg gtggcacgcc ttacaatggt gatggtgcca
     2281 ttgccactga cccatccgtt gttttggaaa cccagttgtt tacggttgac accctgggca
     2341 acatttatac ggcggatggt tcacgaacgc gtcgaattga tgctggcacc ggaattgtca
     2401 ccaccatcat cggaggcgat attgccgggt acagcgacga cggcgatctg gctcagactg
     2461 caaaactatc agatgcccat gaagtcgttt tggatcgggc tggaaacctg tttattgctg
     2521 acaatggtgg tcgccggatt cgccgggttg atgcgctcac cggtgtcatt accactgtgg
     2581 ttggtggagg gcgcttacgt tcagacaatg cacccgctga taagtttttg ctcgttaaag
     2641 cccaggggtt gaccattgat cacaacaacc ggctgtattt tgaagacctg aatcagattt
     2701 tctcgctgga tcctgaaaca caaacggtgc acattgtggc tggaaccggc caaagaggag
     2761 cgagtgg
//
LOCUS       DAOQDO010000667         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30464_length_2765_cov_3.981181, whole genome shotgun sequence.
ACCESSION   DAOQDO010000667 DAOQDO010000000
VERSION     DAOQDO010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30464_length_2765_cov_3.981181"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PKZ53_11340"
     CDS             <1..590
                     /locus_tag="PKZ53_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41074.1"
                     /translation="YQRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLV
                     KTSRLMVQELGREPSPEEIAQRMEIELSKVRKIMKIAQQPISLETPIGEEDDSHLGDF
                     IEDRTIVNPAETLTLVKLRDTIDDLLKTLTPREEKVIKMRFGLDPGGSEHTLEEVGKH
                     FAVTRERIRQIEAKALRKLRHPSRSRKLKSFMEGI"
     gene            complement(781..1359)
                     /locus_tag="PKZ53_11345"
     CDS             complement(781..1359)
                     /locus_tag="PKZ53_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41075.1"
                     /translation="MDKRKFERYLKTKSKKELMAEIILLSNEYETVQEHFEAKMQADA
                     IVRQLKRYKKAISEVFKVQSVTDLPNLELVQDVITQFQKSAKTPEYIAELMVYALEES
                     VKFARKFGPTDESYTEKLQHLYQDTVLFVEKQGLGNHIALRLKTIVVQLSSLFFRWDY
                     FIPASIAKYFTRSELKEIEPKKRAKAKTTKKR"
     gene            1692..>2765
                     /locus_tag="PKZ53_11350"
     CDS             1692..>2765
                     /locus_tag="PKZ53_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41076.1"
                     /translation="MVVQTLKLSLRCVLMVVALCLTLLQVWATPPTPPVKFHHLSIEQ
                     GLSQSSILCIWQDQQGFLWFGTQDGLNRYDGYTFLVYRNDPQLLTTLSSNRVGTLFQD
                     QSKQLWVGTNNGLNRFDPSTGTFTRFLNQPQDPTTVSSNRISAICEDQTGRLWIGTAD
                     QGLNCLNPTTGFKRFQHQPHNANSLSGDQITALFCDRAGVLWIGTAQDGLNRFDPTTG
                     NFIRVPINSQNPSENISGQIRVISEDQTGALWVGTADQGLFQIDRTTGTTTHFFNDPA
                     NPLSLSNNSVRALLLDRKGAVWIGTDTGLNRFDPSSRTFSRFVNDPRNPLSLSHNDIR
                     SVYEDQAGSLWVGTLGGGVNRLDQ"
BASE COUNT          708 a          734 c          652 g          671 t
ORIGIN      
        1 aataccagcg ggggtataag ttctcaacct atgcgacctg gtggattcgc caggccatca
       61 cccgcgcgat tgccgatcag gcccggacga tccgtattcc ggtgcatatg atcgagacca
      121 tcaacaaact ggtcaaaacc tcacgcctga tggtgcagga actcgggcgc gaaccatcgc
      181 ctgaagaaat cgcccagcgc atggaaatcg aactttccaa agtgcgcaaa atcatgaaaa
      241 tcgcccagca gcccatcagc ctggaaacac cgattggtga agaagatgac tcgcacctgg
      301 gggacttcat cgaagaccgg acaattgtca atccggctga aacactgacg ctggtgaagt
      361 tgcgcgatac cattgatgat ctgctcaaaa ccctgacacc ccgtgaggaa aaagtcatca
      421 aaatgcggtt tggtctcgac ccagggggct cagagcatac actcgaagaa gtcggcaaac
      481 actttgccgt cacccgcgaa cgcattcgtc aaattgaagc caaagccctg cgcaaattgc
      541 gtcatccttc gcgtagtcgc aaactcaagt cgtttatgga aggaatttga cgagggatca
      601 ggggtttggg gaaaaaacaa aagggaccaa aaggaccaaa aggacataaa ttcgaaaacc
      661 ctgaacccga aaccccgaac ccaaaaccct gacaagatga ccgactgaca aggtgacggg
      721 tgattttatc atccttcatc cctcatccct tccgaaaacc cccgaacctg ccctgcttgc
      781 tcatcgcttt ttcgtggttt ttgcctttgc tcgtttcttc ggctcaatct ctttcaactc
      841 gctacgtgtg aaatattttg caattgaggc tggaataaaa taatcccacc gaaagaacaa
      901 acttgataat tgcaccacaa ttgttttcag ccgaagcgca atgtggttgc caagtccctg
      961 tttttccaca aacaagacag tatcctggta cagatgctga agtttttcag tgtaggattc
     1021 gtctgtcgga ccaaactttc gggcaaattt gacgctttcc tccagggcat agaccatcaa
     1081 ctcagcaatg tattcaggcg ttttggccga tttctgaaac tgcgtaatga catcttgaac
     1141 cagttccaga ttggggagat cagtcacgga ttggaccttg aatacttcgc tgatcgcttt
     1201 tttataccgc ttcagttgcc gaacaatggc atcagcctgc attttggctt caaaatgctc
     1261 ttgaaccgtc tcatattcgt tcgaaagcag aataatttcg gccatcaatt ctttctttga
     1321 cttggtcttt aaataccgct caaatttgcg cttatccata atgcttccat agagttgatt
     1381 cagatttttt ggcgtgcggt caggcgaggc cgcgttggga tcctgaaaac ggaggcggta
     1441 ccaggtttga gaaaatgaac cggatgagct tattcaatct gctgagcctt gagaagacaa
     1501 aatcaaagcc tggatatctc ccggccagtc tcaaccctgg agttcgaccg tgatgtggat
     1561 ggcacaaagc gagccgtcca ggtcatatca gagttcccaa aacttgcgaa aatttcacgt
     1621 aaccctatac tgtcctccac ttaagtcaat tcctgacctt ccggtttcct tcttttcccg
     1681 agacccagtt tatggttgtg caaacgttga agctgagcct gcgctgtgtg ctcatggtcg
     1741 tggcactgtg cctgaccttg cttcaggttt gggccacacc gccaaccccg ccggtaaagt
     1801 ttcaccacct ctcaatcgaa cagggacttt cccaaagttc aatcctctgt atctggcagg
     1861 atcagcaagg ctttctctgg tttgggaccc aggatgggct caaccgctat gatggataca
     1921 cgtttctggt ctaccgcaat gatccgcaac tccttaccac tttgagcagt aaccgggtgg
     1981 ggactctatt tcaggatcaa tcaaaacagc tctgggttgg taccaacaat ggactgaatc
     2041 gctttgatcc atcaactggc acatttaccc gatttttgaa ccagccacag gatccaacca
     2101 cggtgagcag taatcggatt tcagccattt gcgaagatca gactggacgg ctctggatcg
     2161 gcaccgctga tcagggattg aactgcctca acccgacgac tggattcaaa cgatttcaac
     2221 accagcctca caatgccaat agtctgagtg gtgatcaaat cacggcgtta ttttgtgatc
     2281 gggctggggt gctctggatt gggacggccc aggatggact caaccggttt gacccaacga
     2341 ccggaaactt cattcgcgtc ccaatcaact ctcaaaaccc gtcggagaac atcagcgggc
     2401 agattcgggt gatcagtgaa gaccagaccg gcgccctctg ggttggcacc gccgatcagg
     2461 ggctttttca aattgaccga accaccggca cgaccaccca cttttttaat gatccagcca
     2521 atccgctcag tttaagcaat aacagcgttc gagcactcct ccttgatcga aaaggcgcgg
     2581 tttggattgg caccgatacc gggctcaatc ggtttgaccc atccagccgg acgttctcac
     2641 ggtttgtcaa tgatccaaga aatccattga gcctcagtca caatgatatt cgctctgtgt
     2701 atgaagacca ggctggctcg ctctgggttg ggacgctcgg tgggggtgtc aaccggttgg
     2761 atcaa
//
LOCUS       DAOQDO010000668         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30468_length_2765_cov_3.085240, whole genome shotgun sequence.
ACCESSION   DAOQDO010000668 DAOQDO010000000
VERSION     DAOQDO010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30468_length_2765_cov_3.085240"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2765
                     /locus_tag="PKZ53_11355"
     CDS             <1..>2765
                     /locus_tag="PKZ53_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41077.1"
                     /translation="GVVALAPDAPFGLPVFRTITERDGLPQNSIQKLAFDPKGYLWVA
                     TQDGPAYYNGRVWVRVDLPNRAVSNNVRAMVVASDGSIWFGTDGGGVLQHKNGEWHSF
                     TEASGALPSNQVRCLVETWSISGQSMIWVGTRNGLACLEAGKWTHYTTKSGLPNNWVL
                     SLLETRGANGNRQLWVGTDGGGLACFEQGEWSVYSVESGALPHNGVLSLLETTASTGT
                     RTLWVGTSGGLTRFENGQWTTFDVKSGLPNNIVLTLLETRSVTGQSTLWIGTYGGGLI
                     RLDEKQAGESAFSIPLSAAGQTTLQKSYTVSVYDTRSGLPNNVVYSLLESKAETGIRT
                     LWIGTLGGGLARLEQAKWTAFNTRTGLPNDLVYSLLETKSADGNSIVWAGTDSGLARL
                     ERGQWTLFNSGAGLPTNVAYSVLETTSREGTKSLWVGTYGGGLACLENGKWTIYNTTN
                     GLPHNIVRGLHQSVLPGGERYLWVATDAGVARLSLQGATSPSVKTGSLGQRPPWLIFD
                     MQTGLPNSVARCLTETVAASGERSVWVGTDSGLARLDFQSEIKFTAVTDGPTRPVLSL
                     LSTVSADGSYWLWAGTEGGGVWRCNVARPGSKWESLTKEQNPRMLSNTVNQLREDSQK
                     RLYLFTKKGITRLTPQASRPNQLAEFDCYTFTTDDGLPSNECTRGASLVDSHGRIWAG
                     TLAGVALYDPAQEVNDRRKKPLFIETTLVNNHPVSLEQLTGQSLSYSQNNLVFEYALL
                     SFFRESDTRYQTQLVGYDAAVTDWSATPQRVFTNLPENTYTFRVWGRDYAGNISGPVD
                     VSFQIRPAPWRTWWAYLIYLAGVIGLAWWMMQWQVRSFQRRNEELESKVAARTAELTL
                     TNDELDRKNAEIAKKNAELALNIEQLSLSESRALHSEQEALAAKEEALQASRANAAQS
                     AAQAAER"
BASE COUNT          634 a          713 c          805 g          613 t
ORIGIN      
        1 cgggggtggt tgctctggca ccagatgctc catttgggtt gccggttttt cgaaccatca
       61 ccgaacgtga tggattgcct cagaattcaa tccaaaaact ggcttttgac ccgaaaggct
      121 acctctgggt cgccacccag gatggaccag cttattacaa tggacgggtc tgggtcaggg
      181 ttgatcttcc aaatcgagct gtctcaaaca atgtccgggc catggttgtc gccagcgatg
      241 gcagtatctg gtttggcacc gatggcggcg gtgtattgca acacaaaaat ggcgaatggc
      301 attcctttac cgaagcgagt ggggccttgc ccagcaatca ggtgcggtgc ctggtggaaa
      361 cctggtctat ctctggccag agcatgatct gggttggaac tcgcaacggg ctggcctgcc
      421 tggaagccgg gaagtggacg cattacacca ctaaatctgg attgcccaat aactgggtgt
      481 tgagcttgct tgaaacgcgc ggggccaacg gcaaccgaca actctgggtt ggtactgacg
      541 gtggcggatt ggcctgtttc gagcagggag aatggtcggt ctattcagtt gaatcaggcg
      601 ccttgccaca caacggcgtg ctcagtttgc ttgaaaccac ggcttcaacc ggtacccgga
      661 cgctgtgggt tgggacctct ggtggcttaa cccggtttga aaatggtcag tggacgacat
      721 ttgatgtgaa atcagggttg cccaacaaca ttgtgctgac gttgcttgag acccggtcag
      781 tcacgggaca aagcaccctc tggattggaa catatggggg cgggttgatt cgcctggatg
      841 agaaacaggc cggagagtcg gcattttcca ttcctttatc agcagccggt caaaccacac
      901 ttcaaaagag ttacacggtt tcggtgtatg atacccggtc tggcttgccc aacaatgtgg
      961 tctacagttt attggaaagc aaagctgaaa ccgggattcg gaccttgtgg atcggcaccc
     1021 ttggtggtgg gttagcccgc ctggagcaag ccaaatggac tgcgtttaat acccggaccg
     1081 ggctccccaa tgatctggtg tacagcctgc ttgaaacaaa gagtgctgat gggaattcca
     1141 tcgtttgggc tggtactgat agcggactgg cccgactgga gcgcggacaa tggacgctct
     1201 ttaactctgg cgcagggttg cccaccaatg ttgcctacag cgtgctcgaa accacctcac
     1261 gtgaaggtac caaatccctc tgggttggaa cgtatggggg aggactggcc tgcctggaaa
     1321 atggaaagtg gacgatttac aacaccacca atggattacc acacaatatc gtgcgtggac
     1381 tgcatcagag tgtgctccca ggtggcgaac ggtacctgtg ggttgccact gacgctggag
     1441 ttgcccggct ctcattgcaa ggcgccactt caccgtcggt taaaaccggg agtttaggtc
     1501 agcgacctcc ctggctgatt tttgacatgc agaccgggct tccaaatagt gttgcccgat
     1561 gtttgaccga aacggtggct gcctctggtg agcggagtgt ctgggttggc acggatagtg
     1621 gtctggcccg gctggatttt caatccgaga taaaatttac ggcagtgacc gatggaccaa
     1681 cccgcccggt cctcagtttg ttgagtactg tctcggcaga tggcagttac tggttgtggg
     1741 ccggaacaga aggcgggggc gtctggcgct gtaatgtcgc tcgacctgga agtaaatggg
     1801 aatcgctcac caaggaacaa aacccacgga tgttgagcaa taccgtgaat cagctccgcg
     1861 aagacagcca gaaacggctg tatctcttta ccaaaaaagg aattacgcgg ctaaccccac
     1921 aggcttcgcg cccaaatcaa ctggctgaat ttgactgtta cacctttacc actgatgacg
     1981 gcttgccaag caatgagtgt acccgtgggg cgtcgctggt tgatagccat ggtcggattt
     2041 gggcgggaac gctggctgga gtggccctct atgacccggc ccaggaagta aacgaccgcc
     2101 gcaagaaacc gctgtttatt gaaaccacac ttgtcaataa ccatcccgtc agtctggagc
     2161 agctcactgg ccagtcgctc agctatagtc agaacaatct ggtttttgaa tatgccttgc
     2221 tcagtttttt ccgagagtcg gatacacgct accagaccca actcgtcggc tatgatgctg
     2281 cggttaccga ctggtcagcc acgccacagc gtgtgtttac caatttgccg gaaaatacct
     2341 atacctttcg agtttggggg cgtgactacg ctggaaatat cagcggaccg gtggacgtct
     2401 catttcaaat ccggccagct ccctggcgaa cctggtgggc ttatttaatc tatcttgccg
     2461 gagtgattgg gttggcctgg tggatgatgc agtggcaggt acggtctttt cagcgacgga
     2521 atgaagaact ggaaagcaaa gtggccgcgc gaaccgcgga actgaccctg accaacgacg
     2581 aacttgaccg caaaaatgcc gaaattgcca aaaagaatgc cgaactggca ctcaatatcg
     2641 agcagttatc cctctcagaa agccgggcgc ttcattcaga gcaggaggca ctggcggcaa
     2701 aagaagaagc cctgcaggcc agccgcgcca acgccgccca gagcgccgcc caggccgccg
     2761 agcgc
//
LOCUS       DAOQDO010000669         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30639_length_2756_cov_1.794891, whole genome shotgun sequence.
ACCESSION   DAOQDO010000669 DAOQDO010000000
VERSION     DAOQDO010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30639_length_2756_cov_1.794891"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKZ53_11360"
     CDS             <1..653
                     /locus_tag="PKZ53_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41078.1"
                     /translation="ESALGHLPGLAPAPSGTNANSFGYSVHARILPYIEQENLGRTFD
                     INTTPLFTGTFPVSLTLNPAIAATAATPVKTFLCPADGQSPLYTVITGGGTHAGTNYV
                     VNLGSDGVTRSLIYPDPGKPARVLPDEPMTIGPLRIDPPAGTDKLTVLFSKTRIASFE
                     DPTKDLNKAFLEELSSRTYTTIEEEEIYLPSKEILAMYFANKGDLLKAEIDVRHFE"
     gene            801..2681
                     /locus_tag="PKZ53_11365"
     CDS             801..2681
                     /locus_tag="PKZ53_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41079.1"
                     /translation="MSSNAFEFKPNDKLGDRYVIQAKLGEGGMGTVYRALDERNLNRP
                     VAIKLINAKANQRFAVKARQRLLREREAYLRITHPNVVQVIDSGVTDDGNLFLVQEYI
                     AGESLDKLIRRKGQLPKNEILSMVGQMSSALEAIHSEGVLHRDLKPTNIMLQNLSGGG
                     KQVKIIDLGIARVEDSVIEDSLTVEGASIGTPWYSSPEQLTGAVATPLVDLWALAVIA
                     YQLVTGVAPFREPTIVLLVRRQLSGPISPKQLRPDLPEAVSTVLMKALSYEPSKRYQT
                     VREFSTALNLAFAGSSGTQTSNQPGLNPIEVVEEVEKDTDPTLAPANKKETEAAKVAQ
                     MAALRETAEKPLSIAANQEVAQAGDLSIRQESSQPAMSSLGGVTPQSGLSVPSQIVVS
                     PSQIQVQVVLPTPSWRHPRMWFFIIAVVAVIGVAAWMGRQPERPPVPPPHQGTGTNPI
                     VTPATSSVSTSLSYSLIVDDQRGAPFNASGREIYRSGDSFKFVVETTETGYLYLLNES
                     TSGDLVQLFPARHLNQENSMVGGNQKLSLPQQWYQFDKSVGAERLILVFSRTPVELLE
                     KLQTKKLTSPEGIITSNQSLQIREWIKSNQTDIKQTNLDFETRLESATNPLVAQITLQ
                     HR"
BASE COUNT          694 a          730 c          671 g          661 t
ORIGIN      
        1 atgaatcggc gctggggcac ttacctggcc tcgcgccggc tccgtcgggc accaacgcga
       61 actcgtttgg ctattcggtc catgcccgca ttttgcccta catcgagcag gagaacctcg
      121 gtcggacatt cgacatcaac actacgccgt tattcacggg aaccttcccc gtaagcctga
      181 cactcaatcc cgccatcgct gcgaccgctg ccacgccggt gaaaacgttc ctctgtcccg
      241 ccgatgggca atcgcccttg tacacggtga ttaccggcgg aggcacgcat gcggggacga
      301 actatgtcgt caaccttggt tccgatggtg ttacccggtc attgatttac ccggatccag
      361 gaaaacctgc ccgcgttctg cctgatgaac caatgacgat tggtccatta agaattgatc
      421 ctccggctgg aaccgataaa ttaacggtgc tgtttagcaa aacccggatt gcttcgtttg
      481 aagacccaac caaagacttg aacaaggcat ttcttgaaga attaagttca cgtacctata
      541 ccacgattga ggaagaggaa atctatcttc cttcaaaaga aatcctggcg atgtactttg
      601 ccaataaggg tgatttactt aaggctgaga ttgatgtccg gcactttgag taaagagttc
      661 ttgatttatt gttgaagaaa agttcagggt gcaggtgtgc ccacgtctgt accctgaacc
      721 ggtttttttc ggtcattgcc tcgcatcaga ccagattgat tgatctatct ccatctccct
      781 cgatagctgc ttcagttcgc atgtcatcaa acgccttcga attcaaacca aatgataagt
      841 taggtgatcg ttatgttatc caggccaagc tgggcgaagg tggcatggga acggtctatc
      901 gagccctgga tgagcgaaat ttaaatcggc ctgtcgccat caaattgatc aatgccaaag
      961 ccaatcaacg ctttgccgtc aaagcccgtc agcgattgct cagagaacgt gaagcctatc
     1021 tccgaattac ccaccccaat gtggttcagg tcattgatag cggagtgacc gatgatggaa
     1081 acttgttttt ggttcaggag tacattgccg gcgagtcgtt ggataaactc atccgtcgca
     1141 agggtcagct tcccaaaaac gaaatcctct cgatggttgg ccagatgagt agcgctctcg
     1201 aagccattca cagtgaaggt gttctccacc gcgatttaaa gccgactaat atcatgctcc
     1261 aaaatctttc cggaggcgga aagcaggtga agatcattga tttagggatt gcccgggttg
     1321 aagactcagt gattgaagac tcgctcacag ttgaaggcgc cagcattgga accccctggt
     1381 attcctcccc ggaacagtta accggcgctg ttgccacccc gctggtggat ttgtgggcgc
     1441 tggctgtgat tgcttaccag cttgttacgg gggtggcgcc gtttcgagaa cccacgattg
     1501 ttctgctggt tcggcgtcag ctttcaggcc caatttcacc taaacagctt cgccctgatt
     1561 tgcccgaagc ggtcagtaca gtgttgatga aggcgctgag ttatgaaccc agcaagcggt
     1621 atcagacagt ccgcgagttc tcaacggctt taaaccttgc ctttgctgga agttcaggaa
     1681 ctcaaaccag caaccagcct ggacttaatc caattgaagt ggtcgaagag gttgaaaaag
     1741 atactgaccc aaccctggcg ccagccaaca aaaaagagac cgaagcggca aaagtagctc
     1801 aaatggccgc gctccgcgaa acggctgaaa aaccgctgtc tattgccgca aatcaagaag
     1861 tggcccaggc cggtgacctc tcaattcgtc aagaaagttc ccaacccgcc atgtccagtt
     1921 tgggcggagt aacgccccaa agcggtttat ccgtaccgag tcagattgtc gtttcacctt
     1981 cgcaaatcca ggttcaggtt gtacttccaa ccccgtcctg gcggcaccct cgcatgtggt
     2041 ttttcattat tgccgtggtg gctgtaattg gggtggcggc ctggatggga aggcaacctg
     2101 aacgaccacc agttccgccc ccgcatcagg gaacgggaac aaatccaatc gtgaccccgg
     2161 cgacttcatc ggtctcaacg agtctgagtt attcgctcat tgttgacgac cagcgcggcg
     2221 ctccatttaa tgcgtcaggt cgcgaaattt atcgctcggg tgactcattt aaatttgtgg
     2281 ttgagacaac agaaacaggc tacctgtacc tgttgaatga atccaccagt ggtgatctgg
     2341 ttcaactctt tccagcacga cacctgaatc aggaaaactc catggtcggt ggtaatcaga
     2401 aactttcact gcctcagcag tggtaccagt ttgacaaatc agttggtgca gaacggttga
     2461 ttttggtttt ttcacgaacc ccagttgaat tgctcgaaaa attgcaaacc aaaaaactta
     2521 cctctcccga gggcatcatt acttcgaatc agtcactcca aatccgtgag tggatcaaat
     2581 ccaatcaaac tgacatcaag caaacaaatc ttgattttga aacccgcctg gaatcagcaa
     2641 caaacccact ggttgcacag ataaccctcc aacaccggta gatgacaagg tgacaaggtg
     2701 taagggcgtt gttgctttga accacgtccc cttcgaaaac cctgaaccct gaaccc
//
LOCUS       DAOQDO010000670         2755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30649_length_2755_cov_3.311852, whole genome shotgun sequence.
ACCESSION   DAOQDO010000670 DAOQDO010000000
VERSION     DAOQDO010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30649_length_2755_cov_3.311852"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..1311
                     /locus_tag="PKZ53_11370"
     CDS             493..1311
                     /locus_tag="PKZ53_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ41080.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLSASLAVGEVKTLVVDLDPQAN
                     STSGLGIQRGSFRRSLYNVVILGDMMNNVILPTEIDSLQVGPADRNLAGAEVELVDIA
                     QREYVLKKALDPLRDRYDYIIIDCPPSLGLLTVNALSAADSVLVPIQCEYFALEGVSE
                     LLDTLVRIRRSYNPTLAVEGFLLTMFDERTNLSTQVMNDLRDFYGSQVFETAIPRNVR
                     LAEAPSHGKPIILYDVKSRGAESYLRLANEVMSHDKKGIGARAERSASSEFVSR"
     gene            1247..2110
                     /locus_tag="PKZ53_11375"
     CDS             1247..2110
                     /locus_tag="PKZ53_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ41081.1"
                     /translation="MTRKALGRGLSALLPANSYQGDDFLEIDIDRISPNAQQPRTTFR
                     EESLEELAQSIRANGVVQPILVRKHGLGYELVAGERRWRAAQRAGLHKIPAVVKDVPD
                     EKLLELALIENIQREELNPIEEANAYQRLIQDFKLRQEDIARQVGKERSSIANMLRLL
                     RLSPDVQQLVEDSRISMGHARAILSLEDETLQRQVAEDIIAKGLSVRETERIVRRLID
                     GPPQPTAQPKEADPNLKAAETKLQRTLNTKVRITTGAKGGRIEIDYYSDEDLDRIYHQ
                     LMGNHPLDVSE"
     gene            2336..>2755
                     /locus_tag="PKZ53_11380"
     CDS             2336..>2755
                     /locus_tag="PKZ53_11380"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ41082.1"
                     /translation="MRFGKQQPVEHGGMILGDTEIRGEVTFKDLISIEGRLYGRIQSE
                     TGRLVLAEKGFVEGNIEVGSASISGEVQGNVFARERIDIHGTGKIDGDICAPVLTVDA
                     GAKITGRVETVAPGAGIRVGKPNQPQSPKAVEKDEKGG"
BASE COUNT          685 a          716 c          737 g          617 t
ORIGIN      
        1 gatgctggcg gttccaagtt gggcaaatcc ggtcatggac cacagcagca caagaagtac
       61 agtgccaaga aacagagcga tgcgatggga ggtcatctgg gttctctctc tttctcaagt
      121 gaattatttt tgtggaaggt gagctgatcg aaggtggagc cgaaatggaa ggagccttcc
      181 tgtcttaaca caagttcttg accagaacaa tcaagaactt gaatccatca gacccatttt
      241 tgaatcgggg ctggctcaca gccttgtggc ctggagttgc cagaccggcg taccactgtt
      301 ccacgtggaa cattttcctg atgaaacctt gggtttgtgt tccacgtgga acattttgtt
      361 ctcaaaaagg gtgttccctg tcacctgagc ctttcctggg atggggtgct gtggtagatt
      421 cgctccttcc ctcataacgc atctacacgt cacactcaac tcttgaattc acgaacgaac
      481 ctggggggtc gcatggggaa aattattgca attgccaacc aaaaaggtgg agtgggcaaa
      541 accaccactg ccattaacct ttcggcgagt ctggcggtcg gcgaggtaaa aacactggtg
      601 gtcgatctgg atcctcaggc aaattcaacc agcggtcttg gcattcagcg tggctcgttt
      661 cgccgcagtc tctataatgt cgtcatcctg ggcgacatga tgaacaacgt gatcttgccg
      721 actgaaattg attcgctcca ggttgggccg gcggaccgga atctggctgg cgccgaggtg
      781 gagctggttg atattgccca gcgtgaatat gtgctcaaaa aagcgcttga cccgcttcgc
      841 gaccgctatg actacattat tattgattgt ccaccaagtc tggggctcct gaccgtcaat
      901 gccttgagcg cggctgattc agtgctggtg ccgattcagt gcgaatactt tgccctcgaa
      961 ggtgtctctg aattgctcga taccctggtc cgaatccgtc gcagctataa cccgaccctg
     1021 gcagtcgaag gatttttgtt gacgatgttt gacgagcgga caaacctctc gacccaggtg
     1081 atgaatgacc tgcgcgattt ttatgggagt caggtgtttg aaaccgcgat tccccgcaat
     1141 gtgcggttgg ccgaagctcc cagtcacggc aaaccgatta tcctgtatga tgtgaaatcc
     1201 cgtggggccg aaagctatct tcgattagcc aatgaggtaa tgagtcatga caagaaaggc
     1261 attggggcgc gggctgagcg ctctgcttcc agcgaattcg tatcaaggtg acgatttcct
     1321 tgaaattgat attgaccgga tcagcccaaa cgcgcagcaa ccgcgaacca cctttcgcga
     1381 agagtcactt gaggaactcg cccagtcaat tcgcgccaac ggggtcgttc agccaattct
     1441 ggttcgtaaa catggcctgg gctacgaact ggtcgccggg gaacgtcgct ggcgggcagc
     1501 tcaacgggct ggattacaca aaatcccagc cgtggtgaaa gatgtccctg acgaaaaatt
     1561 attggaactg gccctcatcg aaaacattca acgtgaagaa ctcaacccaa ttgaagaagc
     1621 caatgcctac cagcggttga tacaggattt caagctgcgt caggaagata tcgcccgcca
     1681 ggtcggcaaa gaacgatcct cgattgccaa tatgctacgc ctgctgcgtc tctctcccga
     1741 tgtgcagcag ttggtcgaag actcccgcat cagtatggga cacgccagag ctatcctcag
     1801 cctggaagat gaaaccctgc aacggcaggt cgccgaagat atcattgcca aaggactttc
     1861 ggttcgggaa accgaacgga ttgtccgacg cttgattgat gggcctcctc agccaactgc
     1921 tcaacccaaa gaagcagatc ccaacctcaa agctgccgaa accaagctgc agcgcaccct
     1981 caataccaaa gtccgaatta caaccggggc caagggcgga cggattgaaa tcgactatta
     2041 cagcgatgaa gatctggatc gcatttatca tcaattgatg ggcaatcacc cgctcgacgt
     2101 cagcgaatag cacccctgag cgcgttgtga gccgtcagtc ccacctgcct gcgcgggtgg
     2161 gcaattcaat gtgtgagttt gtccttcctc atccgaaaca ctgggagaat gatcaggtgg
     2221 aaggtgacac gatgaatttt ttcatccctc atccttcctc cctcatccct tccgaaaccc
     2281 ctgaccccaa cccctgaaca aaacccttta acctttcaaa aaaggagacc tgtgtatgcg
     2341 cttcggcaaa cagcagccag tcgaacatgg tgggatgatt ttgggagata ccgaaattcg
     2401 cggcgaagtg accttcaagg acctgatcag cattgaaggc cgactctatg gtcgcatcca
     2461 atctgaaacc ggcaggctgg tattggccga gaaagggttt gtcgaaggca acattgaggt
     2521 tgggtcagcc tcgatttcag gcgaagtgca gggcaatgtg tttgcccgtg aacgaattga
     2581 catccatggc accgggaaga ttgacggtga tatttgcgcg ccagtcctga cagtggatgc
     2641 cggggccaag attaccggac gggttgaaac tgttgcccca ggggccggca ttcgagtcgg
     2701 gaaacccaac cagccacaaa gtccaaaagc ggttgaaaaa gatgaaaaag gcggc
//
LOCUS       DAOQDO010000671         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30690_length_2753_cov_2.917717, whole genome shotgun sequence.
ACCESSION   DAOQDO010000671 DAOQDO010000000
VERSION     DAOQDO010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30690_length_2753_cov_2.917717"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKZ53_11385"
     CDS             complement(<1..666)
                     /locus_tag="PKZ53_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ41083.1"
                     /translation="MLLSLISPLALMLTMSSDGAEVKTLLSLFVIFTAAKLAAELFER
                     LHQPAVVGEILAGVIIGPSVLGWIHPTEVTQVLAEIGVILLLFLVGLEINPTSLFKVG
                     RRALLIAISGVILPFLGGWGLMWGLGYTQVESAFIGAALVATSVGITARVLGAMKVLN
                     AQASQMILGAAVIDDILALLVLSGVSSFAQGQVRFLDLAISASISIGFTLFVLFFGSR
                     LMRK"
     gene            827..1519
                     /locus_tag="PKZ53_11390"
     CDS             827..1519
                     /locus_tag="PKZ53_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ41084.1"
                     /translation="MSQHPVPEETLIFEPVVSRPELIKREPTTDLGHRIVSATVEEFA
                     TKGIVGARVAEIARLAGTTDPAFYRYFHGLRQAALFIMSEYYWKPLNIRVSHYLQVTQ
                     DPVGLVEAVVQALIYSSADDPVRPWLAESKVFQIVVAQMRNPFLLPDSILDREYRAFL
                     EKLTGILTVGQNEGLFQTGVRPDILASLLVTTLHAMLMHRIVCPESTQATEEEIKLVA
                     ARLIGRTEKSSG"
     gene            1851..2048
                     /locus_tag="PKZ53_11395"
     CDS             1851..2048
                     /locus_tag="PKZ53_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41085.1"
                     /translation="MAYLMYFLLFVYGVVGVGTMLYVLNIGRNKMATALLAGAFWPLF
                     WITGIDEQNKRMTGSNRQFPR"
     gene            2082..>2753
                     /locus_tag="PKZ53_11400"
     CDS             2082..>2753
                     /locus_tag="PKZ53_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41086.1"
                     /translation="MASHLANLGVSVLFFCLFYSLGTICRRKLLRSDDRLFLLPFDIG
                     IGAILISLVMFGLGILHLFTPVVGNVWLGLGIVSVFNPMLWKTVQQARAKLREIPKPA
                     YPFIALSGILFALAVITALGPPSTKDDLVYHLQVPKAYLQHHGFLEFPSNIYSYFPYL
                     AEMLYLAGMMAGSDRVPALLHCGFGLCVFLVTYLFARQLEVSKLGSWVAVAAVSPPVR
                     MMWMRV"
BASE COUNT          661 a          711 c          669 g          712 t
ORIGIN      
        1 cttgcgcatc aaccggcttc caaaaaacaa aacaaacagg gtaaacccaa tcgaaatact
       61 ggccgaaata gccagatcca gaaatctgac ttgtccctgg gcaaagctac tgacaccgct
      121 caggacgagt aaggccagaa tatcgtcaat aactgccgcg ccaagaatca tctggctggc
      181 ctgagcattg agaaccttca tcgcgcccag gacccgcgcc gtgatgccca cactggtggc
      241 aactaaagcc gcaccaataa aagcactttc aacctgggtg tagccaagcc cccacatcag
      301 gccccagcca cccagaaatg gcaggatcac accggaaatt gcaatcagca gcgcccgccg
      361 gccaaccttg aacagagagg ttgggttaat ttcaagccct accagaaaca ggagcagaat
      421 cacgccgatt tcagccagga cctgggtgac ttccgttggg tgaatccacc ccagcacact
      481 gggaccgatg atcacgcccg ccaggatttc accgaccacc gccggctggt gcagccgttc
      541 aaacagttca gccgccagtt tggcagcggt aaaaatgaca aagagactca gcagagtttt
      601 cacttcggcg ccatctgaag acatcgtcag catcagtgcc agtggtgaaa tgagtgaaag
      661 cagcattgat gactcctttc tgaattgcga taaaccagct cggatggctc aaaactggaa
      721 atggaacaag aaacttgtat ttttataagt aacacttaca tatttgacag caactttcta
      781 acagattaaa cttggcccgt caatcatttt cgatgtggag tcttctatgt cacagcaccc
      841 ggttcctgaa gaaacactca tctttgaacc agttgtttcc cgacctgaac tgatcaaacg
      901 cgagccaaca actgatctcg gccatcggat tgtcagtgcc acagttgaag aatttgccac
      961 gaaaggtatt gtcggcgccc gagtggccga aatcgcccgg ctggctggaa caaccgatcc
     1021 ggcattttat cggtattttc acggactccg gcaagcggca ctcttcatca tgagtgagta
     1081 ttattggaaa ccgctcaata tccgggttag tcattatcta caagtgacac aagacccggt
     1141 tggcttggtt gaagccgtgg tccaggcact gatctattct tcagccgatg acccagttcg
     1201 gccctggctg gcagaatcta aagtttttca aatcgtagtc gctcaaatgc ggaatccatt
     1261 tttgcttccg gactcaattc tggaccgcga atatcgggct tttctcgaaa aactgactgg
     1321 aattttaacc gttgggcaaa acgaggggct gtttcagact ggggttcgtc ccgatatcct
     1381 ggcgtcactg ctggtcacaa ccctgcacgc aatgttgatg cacaggatcg tttgcccaga
     1441 gtcaactcag gcaactgaag aagaaataaa actggtagcc gcacgcctga tcggtcggac
     1501 tgaaaaatcc tcgggctgaa gaagtcgggc ttggggctga agacatcggg ctgaagaagt
     1561 caggcttggg gctgaagaca tcgggctgaa gaagtcgggc ttggggctga agacatcggg
     1621 ctgaagaagt cgggtggaag aactggtttt agttcatccc tcatccttca tccctcatct
     1681 cttggtttca tccctcatcc cttcgattgt cccaagcccg ttttcttcag ccctgagctt
     1741 gcgaatcttc agcccgtttt cttcagcccg aagtcttcag ccctggtttc cctggcaggg
     1801 atagcgcata attgaccttc ccgtctgaat ttttccagtg aggttcttct atggcatatt
     1861 tgatgtactt tttgcttttt gtgtatggcg tggtcggtgt gggaacgatg ctctatgtcc
     1921 tcaatatcgg tcgcaataaa atggcaaccg ccttgcttgc aggcgccttc tggccattgt
     1981 tctggatcac tggtattgac gagcaaaaca aacgaatgac cggaagtaat cgtcagtttc
     2041 caagataaaa acctctcttt tctctggata cccatactgc tatggccagc caccttgcca
     2101 atcttggtgt ttcggttctc tttttctgcc tgttttattc ccttggaacc atttgccgga
     2161 gaaaacttct tcgctcagat gaccgacttt ttttgcttcc ctttgacatc gggattggtg
     2221 ccattctgat ttcactggtg atgtttgggt tggggatctt gcacctcttc accccagtgg
     2281 tggggaatgt ctggcttggg ctcggcattg tttcggtctt caacccaatg ctctggaaaa
     2341 ccgtccagca agcacgtgca aagctgagag aaatccccaa accagcctat ccatttattg
     2401 ctttatccgg aattctcttt gccttggcgg tgataactgc cttaggacca ccaagtacca
     2461 aagatgatct ggtctatcac ctgcaggtgc caaaagcgta tttacagcat catggttttt
     2521 tggaatttcc atcaaatatt tactcttact ttccctatct ggcggaaatg ttgtatctgg
     2581 ctggcatgat ggctggcagc gaccgggttc cagcccttct acattgtgga tttggacttt
     2641 gtgttttcct ggtaacctat ctttttgccc gtcagttaga ggtttcaaag cttggaagct
     2701 gggtggcggt ggcggcagtc tcgccgccgg tgagaatgat gtggatgagg gtc
//
LOCUS       DAOQDO010000672         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30747_length_2750_cov_5.105751, whole genome shotgun sequence.
ACCESSION   DAOQDO010000672 DAOQDO010000000
VERSION     DAOQDO010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30747_length_2750_cov_5.105751"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKZ53_11405"
     CDS             complement(<1..742)
                     /locus_tag="PKZ53_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNJ41087.1"
                     /translation="MCEQVYPSDLTDREWEIVEPELPLKQSRRGRPRVHSLRKVVNGI
                     RYFNREGIRWRSLPKEYPPWKTVHDYLREWGLNGTWQRMHDRLREQVRRAEGRAGQPS
                     AALLDSQSVKTTQEARGDCGYDAGKKIKGRKRHLLVDTLGLLLVVVVHSAAIQDRDGA
                     KLVLTGLRTAWTGLRLIWADGGYSGQLEQWVGEQYPDDPVRLEIVKRSDEQKGFVLLP
                     RRWVVERTFAWLGRFRRLSKDYERRAATS"
     gene            complement(840..2189)
                     /locus_tag="PKZ53_11410"
     CDS             complement(840..2189)
                     /locus_tag="PKZ53_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ41088.1"
                     /translation="MLTLPEEFMNVIVTFAPLFTQPVWTHVQVLLTGAILTPRKRTVS
                     SCLRVMGLGEERHFGNYHRVLNRVRWSGLAASQRLLGLIVTAFAPQGELVCGLDDTIE
                     RRRGDRIAAKGIYRDPVRSSHSHFVKASGLRWLCLMALVRIPWADRVWALPFLTVLCP
                     SERYHQERGRKHQKLTDRARQLLRLVARWLPERQVVVVADSSFAALELLHAVAAKVTV
                     VTRLRLDAALYEPARPRQPGQKGRPQLKGKRLPILAQVATNPATEWTSLTLSRWYNRR
                     NREVEITTGTAVWYHTGKPVVPIRWVLVRDPKGEFEPQAFLSTKLATSPAQVLAWFVQ
                     RWQVEVTFAETRAHLGVETQRQWSQPAIARTTPALMALFSIVTLTAHAMQHQTPIPVE
                     RSAWYTKALPTFSDALAVVRSRLWWEAHFSMSLESKEMEKVPISLLKRLADTLCFAA"
BASE COUNT          576 a          843 c          737 g          594 t
ORIGIN      
        1 cactggtagc ggcgcggcgt tcataatcct tactcaggcg gcgaaaccgg cccagccagg
       61 caaaggtccg ctccaccacc cagcggcgcg ggaggagcac gaagcctttc tgctcgtctg
      121 atcgcttgac gatctccagc cgcaccggat catccggata ctgctcgccg acccactgct
      181 ccaactgccc gctgtacccg ccgtccgccc agatcaggcg cagtccggtc caggcggtgc
      241 gcaggccggt gagcaccagt ttggctccgt cccgatcctg aatggctgct gaatgaacca
      301 ccaccacgag cagcaagccc agtgtatcca ccagcaggtg ccgtttgcgg cctttgatct
      361 ttttgccggc gtcatatccg cagtccccgc gcgcttcctg ggtcgttttg acgctctggc
      421 tgtcaagcag cgctgcgctc ggttgtcctg cccttccttc tgcccgccgg acttgctccc
      481 gcaaccgatc atgcatccgt tgccaggttc cattcaatcc ccattcacgc agataatcgt
      541 gaacggtttt ccacggtggg tattctttcg gcagcgaccg ccagcgaatc ccttcccgat
      601 tgaaatatcg aatcccattc acaaccttcc gcagactatg gacgcgtggt cgtccccgcc
      661 gactttgttt gagcggcagt tccggctcca cgatttccca ctcgcggtcc gttaggtcgc
      721 tcggatatac ttgctcacac atggcagtct ggtttccttg gttcagattt gatcactttt
      781 ccaaagggta ccagactgca actgattttc cggacagatt ctcagctcga ctttggccat
      841 taggcagcaa agcagagcgt gtcggctaaa cgctttaaaa gggaaatggg gactttttcc
      901 atctcctttg attccaacga cattgaaaaa tgagcctccc accacaaacg cgaacggacc
      961 acagccaggg catccgagaa agtggggagc gctttggtgt accaggccga acgctcaacc
     1021 gggatgggag tctggtgctg catggcgtga gccgtcaggg tcacgatgga aaacaaggcc
     1081 atgagagcgg gcgtggtgcg ggcaatggcg ggctgcgacc attgccgctg ggtttcgact
     1141 ccgagatggg cgcgcgtttc ggcaaaggtg acctcaactt gccagcgttg gacaaaccag
     1201 gccaggacct gcgccggaga ggtggcaagc ttggtcgaga ggaaagcttg cggctcaaat
     1261 tcgcctttcg ggtcacggac cagcacccac cgaatgggga cgaccggttt tccggtgtgg
     1321 taccagacgg cggttccggt ggtgatttca acctcccgat tgcgccggtt gtaccagcgg
     1381 gagagggtca gactggtcca ctcggtggcc gggttggtgg cgacctgtgc caggatgggc
     1441 aaccgtttgc ccttgagttg agggcgtcct ttctgccctg gttgacgtgg tcgggccggt
     1501 tcatacaagg ccgcatccaa ccgcaggcgg gtcacgaccg tcaccttggc ggcgaccgca
     1561 tggagcaact ccagcgcggc gaaactgctg tcggccacca cgaccacctg ccgctcaggc
     1621 agccaccgcg ccaccaaccg cagcagttgc cgcgcccggt cggtcagttt ctgatgtttc
     1681 cgcccgcgct cctggtgata ccgttcagac ggacacagta cggtcagaaa gggcaaggcc
     1741 cagacccggt cggcccaggg aatccgcacc aacgccatca ggcacagcca ccgcaaccca
     1801 ctcgctttga caaaatggct gtgggaagac cgcaccggat cccgataaat ccctttcgcc
     1861 gcgatgcggt ctccgcgccg tcgttcaatt gtgtcatcga gtccgcaaac caattccccc
     1921 tgcggggcaa aggcggttac gatcaaaccc agcagccgct ggctcgccgc cagacccgac
     1981 cagcggaccc ggttcaaaac ccgatgatag ttcccgaaat ggcgttcctc acccaatccc
     2041 atcacccgca gacacgaact caccgttcgt ttgcgcggcg tcaggatggc tcccgtcagc
     2101 aggacctgaa catgcgtcca gacgggctgg gtaaacaacg gcgcaaatgt gacgatcacg
     2161 ttcatgaatt cttcaggcag ggtcagcata ggatctcttt cgcaaaaggt tttcccaaac
     2221 ttattctgcc tgattcattt tttcaaagat caaaaatggc caaagtcgag ctaagtccct
     2281 ctcttttcag aaggaaacag gcagtggccg aatgacgcac tgtgccttta caagtgacag
     2341 gccgaagcat aaaattttcc aatggctact attgcatttt ctaatgattt ttcagtcgtt
     2401 tgaagatttg cacggaagct ttgggatttt ctcccaaacc cataatgttt tgggcgcgtc
     2461 ataccggttg ttcacctcga tggtattttt gacgcacctc aaaagtttca aaaaagcaaa
     2521 cagtttcgca atattttcgc aatttttcag tgataacctc accaagccgt tgtacgttgc
     2581 gggggtgatt gcccccacgc ttttcccgca ctgaatgtgc aatgtgcagt cattcatgaa
     2641 aggcaggttg actgtatggc ctctttgaca cagcctccca caactgggaa acccccttcc
     2701 acacccgcat ggaaacgcaa ttcgggtaaa tttctctcca gcacggccct
//
LOCUS       DAOQDO010000673         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30753_length_2750_cov_2.803340, whole genome shotgun sequence.
ACCESSION   DAOQDO010000673 DAOQDO010000000
VERSION     DAOQDO010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30753_length_2750_cov_2.803340"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1073
                     /gene="ric"
                     /locus_tag="PKZ53_11415"
     CDS             321..1073
                     /gene="ric"
                     /locus_tag="PKZ53_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNJ41089.1"
                     /translation="MTVSALTLNPNQTVRDIVVENPQVTLVFDKHGIDYCCGGGKSLA
                     EACQTAGIAVETVQATLDGTLQEVSAREQITDWSKATPGDLIQHIIETHHAFTRFEIA
                     RLGKLLDKVVAVHSANHPELRQVQNIFYALSQDLMPHLMKEEQVLFPFIDRLEKTVTS
                     QMPVPMSCFGTVANPIRMMEYEHDQAGSFLKELRQITHDFTVPEDGCFSYQALYNGLK
                     AFEADLHQHIHLENNILFPKAKQLEGQVFGKQ"
     gene            complement(1096..2319)
                     /locus_tag="PKZ53_11420"
     CDS             complement(1096..2319)
                     /locus_tag="PKZ53_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ41090.1"
                     /translation="MISVHEFSKSIQHARFFRWKLAVVVFLIVTAILCFNPINGYFLL
                     TDADDKATRIAIGVQGLPSLAGVGDFNGDHHPDLLLVGSGADDGHQVDFWSVSFGDGK
                     GGFVLESEFRTMADATSLAIGDFTGDGKDDYAVTYTTTLFLAIHASNGNGGFPFLSGV
                     ELDSLPVGVGAADLNQDRKLDLVVLEAGFGAGSNVAILLGNGDGSFQDPQRITIGRGS
                     RSLVIADINGDQHPDIATANTGSNDVTLLINTTPQPAASPLVFDQTTIRNIGNSPSTI
                     TVGNFNQDGIPDLVVGTTEGVVFLAGNASGLFADAQIVLSGKKVQTGQLYLNSGDFNG
                     DGITDVVASPFGNRTSKIVILFGSASKPLQQKLKLTAGNNPLQVVTGNMDGNHQTDLI
                     VFNRQPQQVTVVLNI"
BASE COUNT          691 a          667 c          680 g          712 t
ORIGIN      
        1 caagtcagta ttttgggcga aatttgggga atgaagaatg aagaattgag aatgaagaat
       61 gacctcaatt tcactgaaat cgagccttcg cggtgacgaa actgacttcc accctgtcct
      121 ttatgttcct tctgtccttt ttgtcctttg atttgggctc aatcaaccac atcagaaacc
      181 acaactgagg cagatgctgg ccggaaagct caatcagatt cttcattctc tgggtgtgac
      241 ttaagtcaat cccaattcca cctgatcggt tgaaaatgag ttccaaccga tattcgatag
      301 tttaaggctg gaggtgaagt atgacggttt ctgcattgac tctgaacccg aaccaaactg
      361 ttcgtgacat cgtggttgaa aacccacagg taacgcttgt gtttgacaag catgggattg
      421 attactgttg tggcggagga aagtccctgg cggaagcctg tcaaacggca ggcattgcgg
      481 ttgaaacggt acaggctacg ctggatggga cgctccagga ggtatccgct cgtgaacaaa
      541 taactgactg gtcaaaggca acccctggcg acctgattca gcatattatt gaaacgcacc
      601 atgcttttac ccgatttgaa attgcacgac ttggaaaatt gctcgataaa gttgtggcgg
      661 ttcacagtgc aaatcatcct gaattgcgtc aggtgcaaaa tattttttat gccttgtcac
      721 aagacctgat gcctcatttg atgaaggaag agcaggttct ttttccattt attgatcgcc
      781 ttgaaaagac agtgacctca cagatgccgg tgccgatgtc ctgttttgga accgtggcca
      841 acccaattcg catgatggaa tatgaacacg atcaggccgg gagctttttg aaagaactga
      901 ggcagatcac gcatgacttt acggttccag aagatggttg ctttagctat caggcgctct
      961 acaacgggtt aaaggcgttt gaagctgacc tgcaccagca tattcacctc gaaaacaata
     1021 ttctgtttcc aaaggcaaaa cagcttgagg gtcaggtgtt tggaaagcaa tgagcccgga
     1081 acccggaacc caacctcaaa tattcaatac cactgtcacc tgctgaggct ggcggttgaa
     1141 aacaatcaaa tctgtctgat ggttgccatc catgtttcct gtcacgacct ggagcggatt
     1201 gtttccagcc gtcagtttca gtttctgctg gagtggtttt gatgcgctcc caaacaaaat
     1261 cacgattttc gaagtccgat ttccaaacgg agatgccacc acatcggtga taccgtctcc
     1321 gttaaaatcg cctgaattga gatacaactg accggtttgt acctttttac ctgacagtac
     1381 aatttgggcg tcggcaaata aaccactggc atttcctgcc agaaaaacca caccctctgt
     1441 ggtgccaact accaggtccg gtattccatc ctggttgaaa tttccaacgg taatggtcga
     1501 aggactgttt ccgatgttcc gaatggtggt ctggtcaaaa acaagggggc tggcggctgg
     1561 ttgcggcgtg gtgttgatga gcaacgtcac atcatttgat ccagtattgg cggtggcgat
     1621 atctggatgc tggtcaccgt tgatgtcggc aatgaccagt gatcgcgacc cgcgtccaat
     1681 ggtgatccgt tgcgggtcct gaaatgaacc atctccgttt cccaggagaa tcgcaacatt
     1741 gctgccggct ccaaaaccag cttcaagcac aaccagatcc agtttccggt cttgattcag
     1801 atcggctgcc cctactccaa ccggcaggct gtcaagctcc acaccactca gaaacggaaa
     1861 gcccccattg ccgttactgg cgtggatggc cagaaacagg gttgtggtat aggtcacggc
     1921 ataatcgtct tttccatccc cggtaaaatc cccaattgcc agcgacgtgg catcggccat
     1981 ggtccgaaac tcgctttcaa gcacaaaccc gcctttgcca tcaccaaaac tcactgacca
     2041 gaaatcaacc tggtgaccgt catcggcgcc gcttccaacc agaagcaaat ctggatggtg
     2101 gtcgccgtta aaatctccga cgccggccag gcttggtaat ccctgaacgc cgattgcaat
     2161 ccgggtggct ttgtcatcgg catcggtcag gaggaaatat ccattgatcg ggttgaaaca
     2221 taaaatcgcg gtcacgatca aaaaaaccac gaccgccagc ttccaccgga aaaaacgcgc
     2281 gtgctggata gatttggaaa attcatggac tgaaatcatg gtgctctctt ccttgcttct
     2341 tttggttctg gttgtttccg gtgatcgagg aggttgccat cctatccctt tttaacgcag
     2401 gagttgttag aacgacgcaa aaagaacgtt aagaatgtta aatgtaagat cagccagtcg
     2461 tcagtcgtca gttgtttacc agggtggagt ttttgaaccc tggaactcct atctttcacg
     2521 cgtggttatt ctgatttcct tccgtcgaga ggggacaggt tgggatttgg attgagcatc
     2581 cgaacggcaa ctcttcgagg tgcaaatcac aaaactacac gtgacagtgg gctctcttgg
     2641 ggacttttat tcagcacttg cttcgtagcg aatgcagagt tgctgtgttg agtaacccgc
     2701 ttccactggt tgccttggtg gttgggactg gattatggtg cgccgtttta
//
LOCUS       DAOQDO010000674         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30842_length_2746_cov_2.725381, whole genome shotgun sequence.
ACCESSION   DAOQDO010000674 DAOQDO010000000
VERSION     DAOQDO010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30842_length_2746_cov_2.725381"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKZ53_11425"
     CDS             complement(<1..565)
                     /locus_tag="PKZ53_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41091.1"
                     /translation="MTLLKPWRNLFSTARHTPWRWKLVLVLIVSLGLFGSAQSARGKA
                     SPFELANALPRGAFVYGQFQDLPEFIKRWNESGLKARYQESVNYQQFQKRHLGLKLME
                     RYVEFNSALGFELDSMTMSNSAEKKAAFAVYDIGRMEMVFVAPMSDEKIAATQFFSNQ
                     DRFEPTELPDGTTCYSLEVEADRGRQKQ"
     gene            complement(659..>2746)
                     /locus_tag="PKZ53_11430"
     CDS             complement(659..>2746)
                     /locus_tag="PKZ53_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41092.1"
                     /translation="IASNFNLESSERYVRILTEHGLSEQGRARLFPILVKELKRIEKE
                     NDEGYESDPNRQFEPLKSLLHTLSASFHPELKDDTPLSPAVAQAQAAYFNQLCLELPK
                     NSWLAETVIGDSLVSETEHARFYQILIDQSSRLDRYSYDYQFTDQLKTTFNPVRAEEI
                     LDHKNDYRVEEPQSDRLKWQQMLLEYQLSQQQTAAALNTVETIENELKTGYPRPEWLR
                     LTRFRLEAQTRTTTLFEELTHFCGIETYPETLTVQGPSLRRVNDVVALLQRENRTDLA
                     QNLLQAAYERMLALEQLEPSAFIGLARLAFERNDAHTGLQFLNFLTSLDEDETRETTQ
                     AAVARLPFLANRVITDPVVEKPDPVLSVSRGEALSLGAELAAEFGQIELAIQYRQRFR
                     TIHPENEINRLELARLLALANHTSEAIAELAAVMGDRSVLRKTRWLAVWLAPELIGTN
                     TARWTTLEQMVQTKGDTEMLTAIQAKKLASINQPSEALQRLNQHQTVLANPYGWFFRA
                     LLEKATHQPGQAIADFSRAQTNTPHFSMSESFGLTEADPQIQQIGLYLDGNQPRAAFS
                     IADKLTSPEVRGQGSGVGGQGSGIKILEDFLEPGARSQGQTLQTLTQNRQRQIQLEIL
                     ERLSLVAEQINAFDIALKYENQRLALLADAAQQLTSEARIEKLSALLAAQPAPPAPAL
                     VIDQRFVTQMNEE"
BASE COUNT          607 a          697 c          749 g          693 t
ORIGIN      
        1 tctgtttttg acggccccgg tcggcttcga cttcgaggct gtagcaggtt gttccatcag
       61 gaagctcagt cggttcaaaa cgatcctggt tgctaaaaaa ttgagtggcg gcaatttttt
      121 catcactcat cggtgcgaca aacaccattt ccattcgacc aatgtcatag acggcaaagg
      181 ccgctttctt ttctgccgaa ttgctcattg tcatcgagtc cagttcaaac ccaagggcag
      241 aattgaattc aacgtatcgt tccatcagct tgagcccgag gtgacgtttt tgaaactgtt
      301 gataattcac gctttcctgg tagcgagctt tcaaacctga ttcattccag cgtttgatga
      361 actccggcaa atcctgaaac tgtccgtaga caaacgcccc acgcggcaag gcattcgcca
      421 gttcaaatgg actggctttt cccctcgcgg actgcgccga accaaaaagc cccaggctca
      481 caatcagcac cagcaccagt ttccaccgcc agggtgtgtg tcgcgccgta gaaaacaaat
      541 ttctccaggg tttcagaagt gtcataaact tgcctcaatt acctcaatca aaaggacata
      601 aaggacaaaa atgaaaagaa ggtgagactg attttcttca ttcttcattc tcaattcttc
      661 attcttcatt catctgagtc acaaatcgct gatcaatgac gagggctgga gcgggcggag
      721 caggttgagc cgccaggagt gccgaaagct tttcaatgcg agcttcactc gttaactgct
      781 gggcggcatc agcaaggagc gccagtcgct gattttcata cttcagcgcg atgtcaaagg
      841 cgttgatttg ttccgccacg agtgacagcc gttcgagaat ctcaagttgg atttggcgtt
      901 gacgattttg agtgagggtt tgcagtgttt gaccctggga tcgagcacct ggttccagga
      961 aatcttctaa aatcttgatc cctgatccct gacccccgac ccctgacccc tgacccctga
     1021 cctctgggct ggtcagtttg tcagcgattg agaaggcggc tcgtggctga ttcccgtcaa
     1081 ggtacaaccc aatttgctgg atctgcggat cggcttcggt caatccaaaa ctctcactca
     1141 ttgagaagtg cggggtattg gtttgagcac gggaaaaatc agcgatagcc tgaccgggct
     1201 ggtgggtcgc tttctccaga agggctctga aaaaccagcc atacgggttt gccaacacgg
     1261 tctggtgctg gttgaggcgt tgaagcgcct ccgatggctg attgatcgaa gccagttttt
     1321 tcgcctggat ggcggtcagc atttcggtat cgcctttggt ttgaaccatt tgttcaagtg
     1381 ttgtccaccg tgcagtgttt gtcccgatca actcgggcgc cagccagacg gccagccacc
     1441 gggtttttcg caagacggag cgatctccca tcacggcagc cagttcagca atggcttctg
     1501 aggtatggtt ggccagggcc agcaagcggg ccagctcaag gcggttgatt tcattttctg
     1561 gatgaatggt tcgaaaccgc tgccggtact gaatcgcaag ctcaatctga ccgaactctg
     1621 cggccaactc agcaccgaga gacagggcct ctccgcgaga aacggaaaga accggatcgg
     1681 gtttttcaac caccgggtcg gtaatgaccc gattggcaag aaatggaagt ctcgccaccg
     1741 ccgcctgggt cgtttctcgg gtctcgtctt cgtccagact ggtcaggaaa ttcagaaatt
     1801 gaagaccggt gtgagcgtcg tttcgctcaa aggccagccg ggcgaggccg atgaaggctg
     1861 aaggttcaag ttgttccagt gccagcatcc gttcataggc ggcctggagc agattttgag
     1921 ccaggtcagt gcggttttct ctttgaagca gcgcaaccac atcattcacc cggcgcaacg
     1981 acggtccctg gacggtgagc gtttccggat aggtttcaat cccacagaag tgggtgagtt
     2041 cttcaaaaag ggtggtcgtt ctggtctggg cttccaggcg gaagcgggtc agccggagcc
     2101 actccgggcg cgggtaaccg gttttgagtt cgttttcgat ggtttcaact gtgttgaggg
     2161 cggcagccgt ctgttgctgc gaaagctggt attcaagaag catctgctgc catttcaatc
     2221 ggtcagattg tggctcctca acccgatagt catttttgtg gtcgaggatt tcttcagccc
     2281 gcaccggatt gaatgtcgtc ttgagctggt cggtgaactg gtagtcatag ctataccgat
     2341 ccagccggga actctggtca atcaaaatct gataaaaccg agcgtgttcg gtttcgctga
     2401 cgagtgagtc accaatcacg gtttctgcca gccagctatt ttttggaagc tcaaggcaca
     2461 attggttgaa gtaggccgcc tgtgcctggg ccacggctgg tgacagcggc gtgtcgtcct
     2521 tcaattcagg gtgaaaggaa gccgaaagtg tgtgaagcag agatttgagc ggttcaaact
     2581 ggcgattggg gtcgctttcg tagccttcgt cattttcttt ctcgatccgt ttgagttctt
     2641 tcaccagaat cgggaaaagc cgtgcccgac cctgctcgct gagtccatgt tcggtcagga
     2701 tgcgaacata gcgttcactt gattcaagat tgaagtttga ggcaat
//
LOCUS       DAOQDO010000675         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30909_length_2743_cov_2.892113, whole genome shotgun sequence.
ACCESSION   DAOQDO010000675 DAOQDO010000000
VERSION     DAOQDO010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30909_length_2743_cov_2.892113"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1541
                     /gene="glmU"
                     /locus_tag="PKZ53_11435"
     CDS             111..1541
                     /gene="glmU"
                     /locus_tag="PKZ53_11435"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNJ41093.1"
                     /translation="MHSPHILIMAAGLGTRMKSPRAKVLHELAGRSLLGHVCHTAIDL
                     SPVQLILIVGHQAQAVEAKIRQELQQFAPDIEQKTKLEFVLQTEQRGTGHAVQMAEPV
                     LAGQNGTVLVLSGDVPLVRPGTLRRLLEVHAENNFAATVLTTDIPDPTGYGRILREAD
                     GTFSRIVEHRDATPQQRTIPEINSGIYAFEINLLLPALARLSPANAQGEYYLTDVLGL
                     LKSDGHRVGIVLHSPPEEVLGINSRIELAETGARLRQETLRHLMRQGVTIVDPASTFI
                     DADAQIGMDTVIYPNVTIEGPTIIGEECVINPGARLVNARLGNKVTVRDYSLVFDSTL
                     EDNTSVGPFAHLRMGAQIASNAVVGNFVEVKKSTLGPGSKAMHLTYLGDATIGSKVNI
                     GAGTVTCNYDGKNKHATIIEDGVKIGSDTMLVAPVRVGKNSVTGAGSVVTRDIPDHSL
                     AAGVPATVKKTFPSDPLAGEHSQTKD"
     gene            1583..>2743
                     /locus_tag="PKZ53_11440"
     CDS             1583..>2743
                     /locus_tag="PKZ53_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNJ41094.1"
                     /translation="MSAEDVAAQLPAKSTIGTSGFTTAYPKLIPGAFAKRIEEESKAG
                     KEFSFNLYTGASTGEMMDGVLARTGAMKKRLPFQSTPDVRDRVNHGDIEFLDMHLSHV
                     PLYIEHGFLEPIDVAIVEAVDVTEDGRIYLTTSSGMSPTFIHYAWKVYIELNEHHPLE
                     LKGFHDIYMPELPPHGRPIHITNVDDRIGVPYVYCPPEKIAGIVLTNTPDEQHDFKPA
                     DETCIRIANHVLEFLQHEIRKGRIPKSYLPFQSGVGNVANAVLAQMARSKHVSRIQMY
                     TEVIQDSIFDLLDAEKLDCASTCALTFSRAGEERFYREINELRQKFIIRPQEISNNPE
                     VIRRMGIISMNTALEVDIFGNVNSTHVMGSKMMNGIGGSGDFHGRQGINIKRD"
BASE COUNT          716 a          737 c          660 g          630 t
ORIGIN      
        1 ccttgttata ctgatttgca ataaaatgaa aacagtcaag taattcaatc aaatcaactt
       61 agtgaactac tggctactga ccactgacta caaactgata ttaggtcact atgcactctc
      121 cacacattct cattatggcg gctggattag gaacccggat gaaatcccct cgggccaagg
      181 tcctgcacga acttgctgga cgcagccttc tgggtcacgt ttgccacaca gccattgatc
      241 tttccccagt tcaactcatt ctgattgtcg gacatcaggc ccaggcagtc gaagccaaaa
      301 tccgccagga acttcagcaa tttgcacctg acatcgagca aaaaacaaaa ctggaatttg
      361 tgctccagac cgaacagcgt ggcaccgggc atgccgtaca aatggcagaa cccgtgctgg
      421 ccggtcaaaa tggaacggta ctggttttgt caggtgatgt gccactggtc cgtcccggca
      481 cgttgcggcg actgctggaa gttcacgctg aaaacaactt tgcggcgacg gtgctcacaa
      541 ccgatattcc cgacccaacc ggatatggcc ggatcctacg cgaagccgac gggaccttta
      601 gccgcatcgt tgagcaccgc gatgcgaccc ctcagcaacg aactattcct gaaatcaatt
      661 ccgggattta cgcctttgaa atcaatctgt tactcccagc tctggctcga ttgtcaccag
      721 ccaatgcaca gggtgaatat tatctgaccg atgtcctggg tctgctcaaa tctgacgggc
      781 atcgggtcgg aattgtgctg cattcaccac cggaagaagt tcttggaatt aattcccgaa
      841 ttgaactggc cgaaaccggc gcccgacttc gtcaggaaac gctccgccac ctgatgcgcc
      901 agggcgtcac aattgtggac ccagccagta cgttcattga tgctgatgcc caaatcggaa
      961 tggataccgt gatttaccca aatgtcacga ttgagggccc gaccatcatc ggtgaagagt
     1021 gcgtgatcaa tccgggtgcc cggctggtca acgccagatt gggaaacaag gtgaccgttc
     1081 gcgattattc gctggtattt gacagcacac tggaagacaa cacgtccgtt ggcccatttg
     1141 cacatttgcg gatgggtgcc cagattgcgt ccaatgccgt agttggaaac ttcgtcgaag
     1201 ttaaaaaatc aacactcggt ccgggcagta aagctatgca tttaacctac ttaggcgatg
     1261 ccaccattgg aagcaaggta aatattggtg ccggaaccgt tacctgtaac tatgatggca
     1321 agaacaaaca cgccaccatc attgaagatg gcgtgaaaat tggaagtgat accatgctcg
     1381 ttgcccccgt ccgagtcgga aagaactcag tcaccggagc tggctcggtc gtaacgcggg
     1441 atattccgga tcacagcctc gctgctggcg taccagcgac ggtgaaaaaa acattcccct
     1501 ctgatccgct cgcaggtgaa cactctcaga caaaggattg atttttatgc gcaggtacaa
     1561 ctcgtttgta cagtcaaagc ttatgtcggc agaagatgtg gcggcacaac ttccagccaa
     1621 aagcacgatt ggcacgagtg gttttaccac tgcctatccc aaactgattc ctggtgcttt
     1681 tgccaaacgc attgaagaag aatcaaaagc cggtaaggaa ttttcattca atctctatac
     1741 cggtgcctcc actggtgaaa tgatggatgg cgttctggcg cgcactggcg ccatgaaaaa
     1801 acgacttccc ttccaatcca caccggatgt tcgagatcga gtcaaccatg gggacattga
     1861 atttctcgac atgcacctca gccacgtccc actctatatc gagcatggtt ttctggaacc
     1921 aattgatgtg gccattgtcg aagccgtgga tgtgaccgaa gatggtcgca tttacctcac
     1981 cacgtcatcc ggaatgagtc cgaccttcat tcactatgcc tggaaggtct atatcgaact
     2041 caacgaacat catccgcttg aactcaaagg gtttcacgac atttacatgc cggaactccc
     2101 accacacggc aggccgatcc acatcaccaa cgtggatgac cggatcggag tgccttacgt
     2161 ttactgcccg cctgaaaaaa tagctggcat cgtgctcacc aatacccctg acgaacagca
     2221 cgactttaaa ccggcggatg aaacctgtat tcgaattgcc aaccatgtgc tcgaatttct
     2281 acagcacgaa atccgcaaag gtcgcatccc caaatcttac cttccctttc agtctggtgt
     2341 cggaaacgtg gccaacgcgg tgctggctca aatggcccgg agcaaacatg tttcccgaat
     2401 tcagatgtat accgaggtca ttcaggattc gatttttgat ttgctggatg ctgagaaact
     2461 ggactgtgcc tcaacctgcg ccctgacttt ttcacgtgcg ggtgaagagc gcttttatcg
     2521 tgaaatcaac gagttgcggc agaaattcat catccgcccg caggaaattt caaacaaccc
     2581 ggaagttatc cgccgcatgg gcattatttc catgaatacg gcacttgaag ttgatatttt
     2641 cggaaacgtc aattccaccc acgtcatggg cagcaagatg atgaacggca ttggcggcag
     2701 cggcgatttt cacggccggc aggggataaa tatcaagaga gac
//
LOCUS       DAOQDO010000676         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30963_length_2741_cov_2.894639, whole genome shotgun sequence.
ACCESSION   DAOQDO010000676 DAOQDO010000000
VERSION     DAOQDO010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30963_length_2741_cov_2.894639"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1920)
                     /locus_tag="PKZ53_11445"
     CDS             complement(208..1920)
                     /locus_tag="PKZ53_11445"
                     /EC_number="2.7.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerone kinase"
                     /protein_id="HNJ41095.1"
                     /translation="MKKLINAPQSVVPEMLEGLVLLYPGLDYVPGQPVLIRSDAADVC
                     QQQVALISGGGSGHEPAHAGYVGRGMLSAAVAGDVFTSPGPDAVFAAMQATAGAPGAL
                     LIVKNYTGDRLNFGLAAERFRAQGNAVEMVIVADDVALADSTDHAGRRGLAGTILVHK
                     IAGAAAEAGASLAEVAAEARAAAAAVRTIGVALTPCIVPAAGKPGFSLAPDEIELGLG
                     IHGESGVRRETLQSADRLVDELLEPLVADLHLRSGERVVVLVNNLGATPTMELAIVAR
                     RALTELERREIIVERAYAGTFLSALEMAGVSLSLMHVDDQQLSRLDALTTAPAWPNAA
                     AQPHRPLRVASATLAETSPPLSTHHPTTHAGRLLKQILTAVFEALIGTADRLTELDRE
                     VGDGDLGISLARGAQAMLNKLPMIDVDDPAQTFYELGSILQQVMGGTSGALYSVFLLR
                     ASRLLHEENQGLAAWVRALHGATEAIREFGGAEPGDRTMLDALLPAGAALQAGFEQGL
                     TVTQALDQAARAATQGAEATQTMSPRRGRSSYVGDRVRGFQDPGAVAVAVWFGVVASE
                     IRQL"
     gene            2364..>2741
                     /locus_tag="PKZ53_11450"
     CDS             2364..>2741
                     /locus_tag="PKZ53_11450"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41096.1"
                     /translation="MQIAACATGFVYRLRWSVGWWVILSIVVMACLPLPVAALNPRKA
                     ITQYIHRTWSKDHGLPSNAILTLLQTRDHYLWIGTYDGLVRFDGASFTIFNKSNTPQM
                     KNNGVWALAEDAEGTLWIGTNGGG"
BASE COUNT          612 a          795 c          721 g          613 t
ORIGIN      
        1 gaaatacaat tctttcaatc atttcgcctt tccataatgc gagaacttca gtccacaatg
       61 gtcttatacc aatttggact gagatccttg tatgagaaaa cagtcaagta attcaatcaa
      121 atagagttag tgaactactg actacgggct actgactaca aactggtatt atttccactt
      181 gagttcatca gtgctttggc tatgggttta aagctgccgg atttcggaag caaccacacc
      241 aaaccacacg gccacggcca cggcacctgg gtcctggaat ccacgcaccc ggtcgccgac
      301 ataacttgac cgtccgcgtc tgggagacat cgtttgggtc gcttcagcac cctgtgtggc
      361 ggccctggct gcctgatcaa gcgcctgtgt cactgtcagc ccttgttcga aaccagcctg
      421 gagggcagca cctgctggaa gcagggcatc gagcatggtt cggtcaccgg gttcggcgcc
      481 accaaactcg cgaatggctt cagttgcacc gtgcaatgcc cgtacccagg ccgcgagtcc
      541 ttgattttct tcgtgcaaga gtcgcgaagc ccgcagcagg aaaaccgaat acagggcccc
      601 ggaggtgcca cccataacct gttgcagaat acttccaagc tcatagaacg tctgggctgg
      661 atcatcaacg tcaatcatcg gcagcttgtt gagcatggcc tgtgcgcctc gtgccaggct
      721 gattcccagg tcaccatcac caacttcccg atccagttca gtcagccggt cggctgtacc
      781 aatcaaggct tcaaaaactg ctgtgaggat ctgtttcaac agacgtccag cgtgagtcgt
      841 ggggtgatgc gttgacagcg gaggactggt ttctgccagc gtggcggagg cgaccctcaa
      901 cgggcgatgc ggctgggcag cggcattggg ccaggctggc gccgttgtca acgcatccag
      961 ccgactgagc tgctggtcat caacatgcat cagcgagagc gaaaccccag ccatttccag
     1021 ggcagaaaga aatgttcccg cataggcgcg ttcgacaatg atttcgcgtc gctcaagctc
     1081 ggtcagggcc cgccgggcca caatcgccag ttccatcgtc ggagtggcgc caagattatt
     1141 gaccagcaca accactcgtt ccccagagcg caggtggaga tccgccacaa gtggttccaa
     1201 gagttcatcc accagccggt ctgctgattg gagtgtttca cgtcggacgc ctgattctcc
     1261 gtgaatgccc aacccgagtt caatttcatc aggcgccaga gaaaatccgg gcttgccagc
     1321 cgctggcaca atgcacggtg tgagcgccac gccaatggtt ctgacggcgg cggcagcagc
     1381 ccgtgcttca gcggcaacct cggccagcga ggcgccagct tcggcggcgg caccggcaat
     1441 cttatgcacc agaattgtcc cggccagccc tcggcgccca gcgtggtcag tcgaatcagc
     1501 cagcgccaca tcatcggcca caatcaccat ttcgaccgcg ttaccctggg cccgaaaccg
     1561 ttcagccgct aacccaaaat tcaatcggtc gccggtgtaa tttttgacaa tcaacagggc
     1621 gcctggtgct ccggcggtgg cctgcatcgc ggcaaaaaca gcgtctggac ctggcgaggt
     1681 aaagacgtca ccagccacgg cagcgctgag catgcctcgc ccgacatacc cggcgtgagc
     1741 tggttcgtgc ccgcttccgc cgcctgaaat caacgcgact tgctgctggc aaacatcagc
     1801 cgcatccgac cgaatcagga ccggctggcc aggcacataa tccagacctg gatagagcag
     1861 gaccagccct tcaagcattt cgggcaccac agattgtggg gcattgatca attttttcat
     1921 gattcaacct ttctgaatgc aaaacttaac ccaattgaca ccaaacccag gtgacgaacc
     1981 ctgactctgg ttgaattcca gatctaaatc aagtcaaagg gtttgatggt ggcttgagtt
     2041 caaccagcag tcaccagata aatatttcct gggagcgccg gcatcctgct ggcaactgat
     2101 tggaaatcaa ccgattgatg ccggccttct caggaaggaa tcttctgctc aggcagttgg
     2161 ctctcaagaa cttccctcct ggctatccag ggtccagaat ctgccccatc atgttctgtg
     2221 ggtgaattct ttcaggctta attcttcatt ctgaggtgct tctggtatga tggactcaca
     2281 ccgctcttca cgttggtgcg tgtgcccttg ctaaaattat tcacgtttct ggattcaatt
     2341 tgatcattcc cataggaatt ctcatgcaga tagcggcttg tgcaacgggg tttgtctatc
     2401 gcctcaggtg gagtgtcggg tggtgggtca tcctgtccat cgtggtgatg gcctgtcttc
     2461 cattgccagt tgcagcgctg aatccgcgta aggccatcac ccaatacatc caccgtacct
     2521 ggtcaaaaga tcatgggtta ccctcaaacg cgattctgac cttgctccaa acccgcgacc
     2581 attacctctg gattggtacc tatgacgggc tggttcgatt tgatggggct tcgtttacca
     2641 tttttaacaa atccaacact ccgcagatga aaaacaatgg cgtgtgggcc ctggccgaag
     2701 atgctgaggg cacgctctgg attggtacca atggtggtgg a
//
LOCUS       DAOQDO010000677         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_30981_length_2740_cov_2.653259, whole genome shotgun sequence.
ACCESSION   DAOQDO010000677 DAOQDO010000000
VERSION     DAOQDO010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30981_length_2740_cov_2.653259"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..591)
                     /locus_tag="PKZ53_11455"
     CDS             complement(103..591)
                     /locus_tag="PKZ53_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41097.1"
                     /translation="MLGLNHYESPLPSPETQYQNDLHQAQTNMRSGVMAGLWFAILAS
                     LGEILSFFLIQSEGVELEGGTLGLIFYVVFVILEISSILTLTAGLKFFHSRFCAVSLL
                     VLFCIIQLFSIVARPTGLVIRIFITIAFLRAFSNGIKGSFEYQRLQKANPALPDWVKT
                     PG"
     gene            complement(1010..>2740)
                     /locus_tag="PKZ53_11460"
     CDS             complement(1010..>2740)
                     /locus_tag="PKZ53_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ41098.1"
                     /translation="NSIARTLAQSFPKENTGYGVTMIPLLDSVFGKVGIALKVLLGAV
                     ILVLLIACVNVANFQLVRMIHRRKELTLRVLLGASRWRLVRMLMFESLTLALVGGLAG
                     LVIALLGVDVLKALAPVDIPRIQTVSVDARVVGFTLLISVLTTLIFGLVPALRVSKTN
                     LEESLRETSAKLAGSSQGTWLRNWFVISEIALALVLMVGAGLMVRTFHQLQHLDPGFN
                     PNQVLTFRLPLSQAKYQTSQDQKQFFEKTLDQVRQLPGVVSAGVVLQRPLSGTVGWDY
                     PFTVEGQSLEEHDQNPYSNYECISPGYLKTMGIPVLAGRDFTDFDRGDQATVVIVGES
                     FAKRYWPGENPVGKRLQFGKPGSKSPMMAVVGVVKDVRYREWDDTRLDIYVPIFQKAE
                     FRADFVLKLSPEVTQAQQADLVKRIQQIVFSIDKDQVISAVTTLNSLVAETLARPKFN
                     AVLLGLLAGFAIVLAVLGIYGVMSYSVTQRTQEIGIRLALGARTSDVIGLVVNHGMRL
                     AVVGITLGLLGSYVLSRFLTSLLFGVQATDPATYILLSLTLLVVARLACYLPARRAAR
                     IDPMVALRCE"
BASE COUNT          749 a          752 c          675 g          564 t
ORIGIN      
        1 gatgagggat gaaggatgag ggatgaaaga aaaccagttc attcttcagc ccgctttctt
       61 cagcccgcag ccccgagctt gcgagtcttc agccccggtt tttcagcccg gcgtcttcac
      121 ccaatcaggg agcgccgggt ttgctttttg caatcgttgg tactcaaacg agcctttaat
      181 cccattggaa aatgcccgga gaaaggcaat tgtaataaag atccgaatga ccaacccggt
      241 aggccgggcc acaatgctaa agagctggat gatgcaaaac aaaaccagca acgacaccgc
      301 acagaatcgg ctgtgaaaga actttaatcc ggcggtcaac gtcaaaatcg agctgatttc
      361 aagtattaca aacaccacat agaaaatgag ccccagcgtc ccaccctcca attcaactcc
      421 ctcagattga attaaaaaga aggataagat ttctcccagt gaagccagga tggcaaacca
      481 cagcccggcc atcacgcctg agcgcatatt ggtctgtgcc tgatggaggt cgttctgata
      541 ttgggtttcg ggactcggca aaggcgattc atagtgattg agaccaagca tggggaactt
      601 ttctccgttc cctggtgcaa aagaggtctt tgaaccaggt tcaatgtctg gctgtaacca
      661 ttgtgtatca agggcttcct gcactcaggc caaaggcatt gcccagtggg actgaacgaa
      721 aacaggttgt cctgaaaagc gattgagcga caaaccaaaa ctggcaaaaa cccggtgttc
      781 gggcaaaagg aaaaagctgc cagccaacgg agtctaaagt gagaacgtat tttgagcaag
      841 cgtgattctg tacaaaaaga gatcaaatga accgggccaa cgactgacga ctgacgggta
      901 caatgccatt ttggaatgca cctccgccgt atcaccaaaa agctcactca gtgaaaaaaa
      961 attgtatttc cctgaaccct gaaccctgaa ccctgaaccc tcaatggtat tactcgcacc
     1021 gaagcgccac cattggatca atccgtgctg cccggcgggc tggcaggtaa caggccaatc
     1081 tggcaacgac cagcagcgtt aatgacagca aaatatatgt cgccgggtcg gttgcttgaa
     1141 cgccaaataa aagtgaagtc agaaaccggc tcaacacata agacccaagc aagcccaggg
     1201 taatcccaac caccgccagc cgcatgccgt ggttgaccac taacccaatc acatcgctgg
     1261 ttctggcgcc aagcgcgagt cgaataccaa tttcctgggt tcgctgggtc accgaatacg
     1321 acatcacgcc gtaaatcccc aggacggcga gcacaatggc aaacccggcc aggagcccga
     1381 gcaacacggc attaaatttg ggtcgtgcca gggtttcagc caccagggaa ttgagtgtcg
     1441 ttacggcgct gataacctga tctttatcaa tcgaaaagac aatttgttga atgcgtttga
     1501 ctaaatcggc ttgttgggcc tgggtgactt cgggcgacaa tttgaggacg aaatcagccc
     1561 ggaactcggc tttttgaaag attggaacat aaatatccag ccgtgtgtcg tcccactcgc
     1621 gatagcggac atccttgacg accccaacca cggccatcat cggtgacttt gagccgggtt
     1681 ttccaaattg caatcgcttg ccgaccgggt tttctcctgg ccagtaccgt ttggcaaatg
     1741 actcaccaac gattactacg gtcgcctgat cgcctcgatc aaagtcagta aaatcacgtc
     1801 ctgccagcac cggaatcccc atcgtcttca agtaccctgg ggaaatgcac tcataattgg
     1861 aatatgggtt ctggtcgtgt tcttcgaggg actggccttc gaccgtgaac ggataatccc
     1921 agccaactgt cccggaaagc gggcgttgaa ggaccacgcc ggctgaaaca acccccggta
     1981 gttgccgcac ctgatccagc gtcttttcaa aaaactgctt ctggtcctgg ctggtctggt
     2041 atttggcctg tgacagcggc aaccgaaacg tcaatacctg gtttgggtta aaacctggat
     2101 ccaggtgttg aagctgatga aaagttcgca ccatcaaccc cgcaccaacc atcaacacca
     2161 gtgccagtgc aatttcagaa atcacaaacc agttgcgcaa ccaggtgccc tggctggaac
     2221 cggcgagttt ggcagaggtt tcacgcaatg attcttcaag gttggttttt gaaacgcgca
     2281 gagccggaac cagtccgaaa atcagcgtcg tcagaaccga aatcaacagc gtgaatccca
     2341 cgactctggc atccactgaa actgtctgaa tccggggaat atcaaccggg gccagtgctt
     2401 tcaatacatc aacgccaagc agcgcaatga ccagtcccgc caggcccccg accagtgcca
     2461 gcgtcaggct ttcaaacatc aacatccgaa ccagtcgcca gcgactggcg ccgagcagca
     2521 cccgtaaggt cagttctttg cggcggtgaa tcatccgaac cagttggaaa ttggctacat
     2581 tgacacaggc aatcaagagc accaggatca ccgccccaag caacaccttg agcgcaattc
     2641 caacctttcc aaataccgaa tcgagcaacg gaatcatcgt cacgccgtac ccggtgtttt
     2701 ccttgggaaa cgactgggca agggtacggg cgatgctgtt
//
LOCUS       DAOQDO010000678         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31016_length_2739_cov_2.451937, whole genome shotgun sequence.
ACCESSION   DAOQDO010000678 DAOQDO010000000
VERSION     DAOQDO010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31016_length_2739_cov_2.451937"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKZ53_11465"
     CDS             complement(<1..576)
                     /locus_tag="PKZ53_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ41099.1"
                     /translation="MPTEVLFDNGVHKCFLLEDFGHGEMVQANQYLIVHDGAGMILDP
                     GGHKVYNKALSETTGLLKGGKLTTIFFSHQDPDIIAAANGWLMTTDAQAYISALWVRF
                     VPHFGLDRLVLDRLHPIPDEGMRYDLAGYQLAFVPGHFLHSPGNFHVYDPESKIYFSG
                     DLGASLGMDYRAVTDFDQHLQYMEGFHRRYMA"
     gene            complement(772..1137)
                     /locus_tag="PKZ53_11470"
     CDS             complement(772..1137)
                     /locus_tag="PKZ53_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNJ41100.1"
                     /translation="MSRTDELNKILRALQSSTPDIEAASIMTDDGLVIASALPQHIEE
                     IRVAGISATILSLGTRATAELERGHLEQVLIRGANGYIVMAAASQGTLLMVMTTREAK
                     LGLIFLDMSRAIQEIKKVL"
     gene            complement(1124..2254)
                     /locus_tag="PKZ53_11475"
     CDS             complement(1124..2254)
                     /locus_tag="PKZ53_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41101.1"
                     /translation="MESRKSVQVLDTLPAGVLVTDVDGVIVYANQKSETILHRSRDQL
                     IGFLLKEILPGMAPHLTMSETRLEEDHQPRCVYKQPDKGDITIGFQIARTGFAEASPR
                     AEFFTVLFQDITGFERLRDERDRLLQLAAVGEVLPGILHELKNPMAAITTAVEVLIED
                     VPEGYVQTELHAILSEVRRMKLAFEGIGLLDLTMCTRKATAIDFAIEEAFRVLEAQMK
                     RNRIVSRCEIQSLPLLYLDPAAIRAIVFNLVNNAIHACKAGDEISVEASLVDHDRVLK
                     IIIRDTGIGMSQDTLRRCRELFFTSKPNGSGMGLALCETVVKRAHGKLLIESREGIGT
                     QVTVLLPVPVPRQPPLGTPLSVQFATTTLESEYSGGRKNVSN"
BASE COUNT          700 a          668 c          686 g          685 t
ORIGIN      
        1 agccatatac cggcggtgaa acccttccat atactgaagg tgctggtcaa aatcagtcac
       61 tgcccgataa tccattccta aggatgctcc caggtcgccg ctaaaataga ttttcgattc
      121 ggggtcgtag acgtggaaat ttcctgggga gtgcaaaaaa tggccgggaa cgaaagccag
      181 ttgatatccg gccagatcat agcgcattcc ttcatcagga attgggtgaa gccgatcaag
      241 taccagccgg tcaagcccaa aatgtgggac aaaccggacc cacagggctg aaatatacgc
      301 ctgagcatca gtggtcatga gccagccatt ggcggcggca atgatgtctg gatcctgatg
      361 tgaaaaaaag atcgtggtta atttcccacc tttgagcagc ccggtggttt cactgagcgc
      421 tttgttataa accttgtgcc ctccggggtc caggatcatt cccgcaccat catggacgat
      481 aagatattgg ttggcctgta ccatctcacc gtgaccaaaa tcttcaagca ggaaacattt
      541 gtgcacccca ttatcaaaca atacttcggt tggcatgatt tttctccttg tcacaatgtg
      601 ctgccacttt ttgaatcaaa ctttcagaca gtcgggcaaa ctcaaaaaaa accgcctcga
      661 aaaacaattg taatatgtag taaggaaaac aatggctcgt aaggaattgt tgaacatatt
      721 gtttttcaaa gggttacgta gtttttttcc tgactgtatg ttcggttaga attacaacac
      781 ctttttgatt tcctgaatgg cacggctcat atcaaggaaa atcagtccca gtttggcttc
      841 acgggtggtc atcaccatga gcaatgttcc ctgcgaggca gcggccatga cgatatagcc
      901 atttgcccca cggatcaaaa cctgctcaag atgcccgcgc tcaagttcag ccgtcgcccg
      961 ggtaccaagg ctgagaatcg tggcactaat tccagccact cgaatttcct caatatgttg
     1021 aggcagcgca ctggcaatga ccagcccatc gtcggtcatg attgaagcgg cttcaatatc
     1081 aggcgtactt gattgcaacg cccgcagaat tttgttgagt tcatcagttc gagacattct
     1141 tccttcctcc tgaatattca ctttccaagg tggtggttgc aaattggact gaaagcggag
     1201 tccccagagg cggctggcgt gggacaggga ccggcagtaa aactgtgacc tgggttccga
     1261 ttccttcgcg actttcaatc aacaatttgc cgtgtgcccg tttgaccacg gtttcgcaca
     1321 gggcgagccc catgccactg ccgttaggtt ttgaggtaaa aaacagttcc cggcaccggc
     1381 gcaatgtgtc ttgactcatt ccgatgcccg tgtcacgaat aatgattttt aagacccggt
     1441 cgtggtccac caggctggct tcaaccgaaa tttcatctcc ggctttacac gcatggatgg
     1501 cattattgac cagattaaac acaatggcgc gaatcgcggc tggatccagg tagagcaacg
     1561 gtaacgactg gatttcgcac ctggatacaa tccgattgcg tttcatttgg gcttccagaa
     1621 cccgaaaggc ttcttcaatc gcaaaatcaa tcgcggtggc cttgcgggta cacatggtta
     1681 aatccagcag accaattcct tcaaaggcga gcttcatccg gcggacttcg ctcaaaatgg
     1741 cgtggagttc ggtttggaca tagccttcag ggacatcttc aatcaggact tcgacggcgg
     1801 tggtaatggc ggccattggg tttttgagtt catgcaggat tccgggcaag acttcgccca
     1861 cggcggccag ttgcagcagg cggtcgcgct catcgcggag gcgttcaaac ccggtgatgt
     1921 cttgaaacag cactgtgaaa aattccgcac gcggtgatgc ttcagcaaat ccagttctgg
     1981 caatctgaaa cccgatggtg atatcgccct tgtcaggttg tttataaaca caccggggtt
     2041 ggtggtcttc ctcaagacga gtttcagaca tggtgaggtg aggtgccatt ccaggcagga
     2101 tttctttgag cagaaacccg attaactgat cccgtgagcg gtgcaaaata gtttctgact
     2161 tttgatttgc gtagacaatc acgccatcta catctgtcac caggacacct gcagggagtg
     2221 tgtctaagac ttgaacagat tttctggatt ccataatgcc atcgggtacc accctctgtt
     2281 gtcgttaaag ccaattttga gaaaacactc gaagcgacag cgactgagac agagggagca
     2341 ggattttctt caatggggat gggtatttca ttggttgcgg caaccttgaa gcgaaaggat
     2401 atgacatcca tgtgcaactc aaaggccaaa actgacgatt attcggtttt tttcggcgaa
     2461 tcaattgaat cgtcagaaac caggccatat caggtggatt ctagtgttag gaatagttcc
     2521 aacctgttga tcccaataga agagccctgc gtgcgattta tgatcctcgg gcgctgaatg
     2581 agtggggaaa aattcgcttc tatgtgcaaa tgttcaaaaa atcgcacctg tactgagaga
     2641 aaattcgagc aggctggaaa aatccacagg ccagaaacgc ctttcttcat cctgtcttcg
     2701 gcgtggagac ccggtccttc agggccggga ggaaacgcc
//
LOCUS       DAOQDO010000679         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31042_length_2737_cov_3.555183, whole genome shotgun sequence.
ACCESSION   DAOQDO010000679 DAOQDO010000000
VERSION     DAOQDO010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31042_length_2737_cov_3.555183"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..404)
                     /locus_tag="PKZ53_11480"
     CDS             complement(18..404)
                     /locus_tag="PKZ53_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ41102.1"
                     /translation="MPRICHFEIHAENPERAIAFYTSVFDWKFQQWGGQPYWLIETGP
                     KEQSGIDGGLLPRHGAPPVGGEPVNAFVCTVDVDDLDAYIVKVTEHGATVAVPKMPIP
                     TVGWLAYYKDTEGNIFGMMQSDPEAK"
     gene            1158..1748
                     /locus_tag="PKZ53_11485"
     CDS             1158..1748
                     /locus_tag="PKZ53_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41103.1"
                     /translation="MDSLPPLPEGFFQGTFSWLSEQNIVSADQYSELEFTLTKMNSLI
                     QQAEQMSFALPPEFVTFFSSSLLPGKIRSCTDCCFALSSRIQPCAGDLGGWVIPVLYD
                     SQGVLFWNLYIGPDGDSCVVYSEEWYDVQPDDEDDEDDEIILAPPTAEDLLYCSPSFE
                     AFVYRFWIENEIWFSLYQNVPLTDLQKKYVSHYRSE"
     gene            1884..>2737
                     /locus_tag="PKZ53_11490"
     CDS             1884..>2737
                     /locus_tag="PKZ53_11490"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNJ41104.1"
                     /translation="MYRPSIRLLATPVEDDLPAGVSKLGGRPDLPDGVDWPTTQIICP
                     RPSEGFLKAHPNERRLPEDGRIHLSFVGQINLKEIQPFDVRSQLPNSGLLSFFYNPQV
                     FASDTNTGSSSGIQDHITGYRYDLFGWDHLANWQVLFTDSDPSELRRREFPNTLYEAI
                     QYKSNRLAFRVENCLPSLETSYFPRPESEDGHIRLTQEEWETYRTLRYDLRANIGIHQ
                     MLGYADQWAATTDEESYWRWRDHLFPELPPEEALSTAERNTEAASIRLLLQVNTFDDT
                     AEWWGRGGT"
BASE COUNT          708 a          628 c          673 g          728 t
ORIGIN      
        1 tctgtggttt ggcctggtta tttggcttcg ggatcagact gcatcatccc aaaaatattg
       61 ccttcggtgt ctttgtagta agccagccag ccaaccgttg gaattggcat tttcgggacg
      121 gccaccgtgg caccgtgttc ggttactttg acaatataag catccagatc atcaacatca
      181 accgtgcaga caaaggcatt gacgggctca ccgccaaccg gcggagcgcc gtgacgcggc
      241 agtaaccccc cgtcaattcc tgactgctct ttgggtccgg tttcaatcaa ccagtacggc
      301 tggcctcccc attgctggaa tttccagtca aacacgctgg tgtaaaaagc gatggcccgt
      361 tcagggtttt cagcatggat ttcaaaatgg caaattcgcg gcatagacac gtctccttac
      421 aagaacaaaa gttcggcctg gaacgatgaa attgtcgaga tggtgaacca gttgagaacg
      481 ctactggtta agcgcgcgaa acataacaca gtaatgcgat tttgtataca aggaatgcag
      541 aatgaagaat tgagaatgaa gaattgagaa tgaagaatga ggaatgaaga attgagaatg
      601 aagaattgag aatgaagaag caaattggtt cttggttctt ggttcttggt tcttggttct
      661 tggttcttga aaagtatcct tctgtaaatt tttccgtgat ggatttcttt ggaacaagct
      721 ggaaatccag gtttagcctg cgaagcaggt accagagagt agcccccggt gcaaccgggg
      781 gaatcgaatg ccgccgcatc cgagcctgcg cagcaggtga cagagagaaa cgaacaccct
      841 ccaggtcacg tgttttcacc ggtatacccc cgattggtgt gaacttcctc aggcactctc
      901 tgccacctgc tgcgcaggct gagaggaaca cccgaccaac cccccggttg caccgggggc
      961 tactgtctcc cacctgcttc gcaggttgaa tcccctgaat ttccagttca tcgaatcgat
     1021 cacgggaaaa tttacagaaa gaaaaatatg aggtttttat gagacgatgt ggagagcttt
     1081 tcaaaaaggg ctggtattca caagaactta aagggtatcg ggatactgag caccacagca
     1141 catatcactt ttttgagatg gattctctcc cacctcttcc tgaaggtttt ttccagggaa
     1201 cctttagctg gctttcagag caaaatatcg tttctgctga tcaatattct gagcttgaat
     1261 ttactttgac caaaatgaat tcgttgattc aacaggcaga gcagatgagt tttgcacttc
     1321 caccagagtt tgttaccttc ttttcgtcga gtttattacc gggcaagatc cgatcttgta
     1381 ctgactgttg ctttgccctg tctagccgga tacaaccctg tgcgggagat ttaggcggat
     1441 gggtaatccc agttctctat gattctcaag gtgttttgtt ctggaacctc tatataggcc
     1501 cggacggtga ttcgtgtgtt gtgtattctg aggagtggta tgacgttcaa ccagacgacg
     1561 aagatgatga agatgacgag atcatcttgg cacccccaac cgctgaagac ttgctgtatt
     1621 gttcgccatc ctttgaggca tttgtgtatc ggttttggat agagaatgaa atctggtttt
     1681 cactgtacca aaatgttccg ctgacagatc ttcagaaaaa atatgtaagc cattacagat
     1741 cagaataatc tatcctttca gttgttcaat tttagcaaaa ggcatcaatc accatgtcct
     1801 cggataatca ccactctggt acggactgtt caagtattcc tgatcaccca ggtttgaaaa
     1861 actgcgctga ggaactgcgt gctttgtatc gtccgagtat cagactgctg gccacgccag
     1921 ttgaagatga ccttccggct ggtgttagta aactcggtgg gcgacctgat ttgccagatg
     1981 gtgttgattg gccaacgaca caaattatat gtcccaggcc ctcagaagga tttttaaagg
     2041 cccatccaaa tgaaagaaga ttgccagaag atggaaggat ccatctttcc tttgtggggc
     2101 agatcaacct gaaggaaatt cagccgtttg atgtcagatc ccagttgccg aactctggat
     2161 tgctttcttt cttctataac ccgcaagtct ttgcatcaga tacgaatact ggttcaagct
     2221 caggtatcca ggaccacatc actggatatc gttatgacct gttcgggtgg gatcatcttg
     2281 ccaactggca ggtgcttttt actgattcag atccatctga attgaggcgg cgcgaatttc
     2341 ccaataccct gtatgaagca attcagtaca aatccaatcg tcttgctttt cgggttgaaa
     2401 attgtttgcc ttcgcttgag acaagctact ttccgaggcc agagagtgaa gacggccata
     2461 tcaggttaac tcaggaagaa tgggagacct atcgaacgct tcgctatgat ctgcgggcca
     2521 acattggaat tcaccaaatg ctgggctatg cggatcaatg ggcggcgacg acagatgaag
     2581 aaagctactg gcgttggcgt gaccatctgt ttcccgaact tcctccagaa gaagcccttt
     2641 caactgctga gcgaaacact gaggccgcaa gtatcagact tttgctccag gtcaacacat
     2701 ttgacgacac ggctgaatgg tgggggcgcg gcggaac
//
LOCUS       DAOQDO010000680         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31053_length_2737_cov_2.530574, whole genome shotgun sequence.
ACCESSION   DAOQDO010000680 DAOQDO010000000
VERSION     DAOQDO010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31053_length_2737_cov_2.530574"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKZ53_11495"
     CDS             <1..821
                     /locus_tag="PKZ53_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNJ41105.1"
                     /translation="AVSGVAMQGLFRNALADPSLIGVSGGAALGATGIIVVSDLAGIS
                     LGTFPGFFALPIAAFVGGLGTTYLILRIAGHTGTTNVSTLLLVGIAINALVQALIGLL
                     TFFATDTALRNLTFWRLGSLGGATWTTVGVMTPFVVMPLVVFPQMGQLLNIYLLGESE
                     AFHLGLNLERLKQTVIILVAVAVGATVSVTGIIGFVGLIVPHLLRQIIGPDHRFLFPA
                     SALLGASLLVLADMIARTIVVPAELPIGIITALIGAPVFLWMLVNDRNLVGTHA"
     gene            814..1602
                     /locus_tag="PKZ53_11500"
     CDS             814..1602
                     /locus_tag="PKZ53_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HNJ41106.1"
                     /translation="MLEARHLTVLLKGNTILSGVSLAVHPGDFIAVVGPNGAGKSTLL
                     KCLSGELAPQSGVVWLNGKPITQVAPRNRAQTLAVLPQQSALQFPFTVCEVVRMGCLP
                     QARSQSRVTEDEIIRQTLEAVEMSHTLHRMYTTLSGGERQRVQLARVLAQLWNRERQH
                     PSFLLLDEPTTSLDLAQQQCILTVARKLSQQGVGVFAVLHDLNLAAQYATKILILHQG
                     KVQQQGTPAQVFTPETIEATFGIPVWVSPHPIGNDLVIVPVANR"
     gene            1775..>2737
                     /locus_tag="PKZ53_11505"
     CDS             1775..>2737
                     /locus_tag="PKZ53_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ41107.1"
                     /translation="MDLFQAQRAKDIEAESPLAARMRPRTLEEFTGQEHILGEGKLLR
                     RAIQADQLSSLIFSGPPGTGKTTLARIIANSTRAAFTSINAVLAGVKEIRDAVSAAQE
                     RRAYQGQRTILFVDEVHRFNKAQQDALLPWVENGTVIFIGATTENPYFEVNKALVSRS
                     RIFQLRSLTTDDLRKIALRALRDADHGYGKVNVQLTEEALEHLVNVANGDARALYNAL
                     ELAVETTQPDAERVIHITLAVAEESIQQRAVLYDKEGDAHFDTISAFIKSLRGSDPDA
                     ALYWLAKMVYAGEDPRFLLRRMLILACEDVGMADPQALGVITACA"
BASE COUNT          578 a          711 c          746 g          702 t
ORIGIN      
        1 tggcggtttc aggggtagcg atgcagggcc tgtttcggaa cgcgctcgcg gatccaagcc
       61 tgattggggt gtcaggcggc gcggcattgg gggccaccgg catcattgtt gtcagcgatc
      121 tggccggaat cagtctggga acgtttccgg gcttttttgc tctgccaatc gctgcttttg
      181 ttggtggact tggcacgacg tacctgattt tgcgaattgc tggccacacc ggcaccacca
      241 atgtcagcac cctgttactg gttggaattg cgatcaatgc actggtgcag gcgctgattg
      301 gtctgctcac gttttttgcg accgatactg ccctgcgaaa cctgactttt tggcggcttg
      361 gaagccttgg cggtgcaacc tggaccacgg tgggcgtaat gactccgttt gttgtgatgc
      421 cgctggtggt ctttccacag atggggcaat tgctcaatat ctatttgctg ggtgaatccg
      481 aagcttttca ccttggatta aatcttgaac ggctcaaaca aaccgtgatt attttggtgg
      541 ctgttgcggt tggggccacg gtttcagtga ctggaatcat tgggtttgtc ggtctgattg
      601 tgccccattt gcttcgacag atcatcggac cggaccatcg gtttctcttt ccggcctctg
      661 ctctgctggg ggcttcgctt ctggtgctgg ccgatatgat tgcccggaca attgttgttc
      721 cggctgaact tccaattgga attatcaccg ctttgattgg tgctcctgta tttctgtgga
      781 tgctggtcaa cgacaggaat ttggttggaa ctcatgcttg aagcacgtca tctcaccgtt
      841 cttttgaaag gaaacaccat tctcagcggc gtttccctgg cagtacatcc aggcgatttt
      901 attgcagtgg ttgggccgaa tggtgccggg aaatctacat tattaaagtg tttaagcggt
      961 gaattggcac cacaatctgg tgtagtctgg ctcaacggaa agccgattac ccaggtggcg
     1021 ccacgcaacc gggcgcaaac cctggccgtg ctgccccaac agtcagcctt acagtttcca
     1081 tttactgttt gcgaagttgt ccgcatggga tgcctgcctc aagcgcgttc tcagtcacgt
     1141 gttaccgaag acgagattat tcggcaaacg ctggaagctg tggagatgag tcacaccctg
     1201 catcgaatgt acacaacact ttctggggga gaacgccagc gagttcagct tgcccgggta
     1261 ttggcccagc tctggaacag agagcgccag catccatcat ttctcctgct ggacgaaccg
     1321 accacaagcc ttgatctggc acaacaacag tgcatcctga ccgttgcccg aaagctgtct
     1381 cagcagggag tcggtgtttt cgctgtcctt catgacttaa atctcgccgc ccaatacgcc
     1441 accaaaattc tgattctcca tcaagggaaa gtgcagcagc agggcacccc agcccaggtc
     1501 ttcactccgg aaaccatcga agccaccttt ggaattcccg tttgggtgtc acctcaccca
     1561 attggaaatg atctggtcat tgttcccgtg gcaaaccgat aaaggtgtgg acaagggttt
     1621 ctggtcattt tcttttgcca gacctgatct acctgtgcct gaatgaggtt gctccaccat
     1681 tccttcgtct gttattactc cagcatgctc tttattctga acaccaacca accccgcctc
     1741 aaaatccaac cttgagcccc gctgtgggat acctatggat ctttttcaag ctcagcgcgc
     1801 caaagatatt gaagctgaat cacctctggc ggcccggatg cggccccgca ccctcgaaga
     1861 attcacgggt caggaacata ttttaggaga agggaagttg ctgcgccggg ctattcaggc
     1921 cgatcagctt tcctcgttga ttttttcagg gccacccgga actggaaaaa ccacactggc
     1981 gcgaattatc gccaattcaa cccgggcggc gtttacttcg atcaatgcgg tgctggctgg
     2041 cgtcaaagaa atccgggatg cggtttcggc agctcaggaa cggcgggcct atcagggaca
     2101 gcggaccatt ttgtttgttg atgaagtgca tcggtttaac aaagcccagc aggatgcgtt
     2161 actcccctgg gtcgaaaatg gaaccgtgat ctttatcggt gccaccactg agaatcctta
     2221 ttttgaagtc aacaaagcac ttgtgagtcg gtcccggatt tttcaattgc ggtccttaac
     2281 cactgatgat ttgcggaaaa ttgcccttcg ggcgcttcgg gatgctgatc acggctatgg
     2341 aaaggtcaac gtgcagctta cggaagaggc acttgagcat ctggtcaatg ttgccaacgg
     2401 ggacgccaga gccttatata atgcgctgga actggccgtt gaaacgactc aaccagatgc
     2461 tgaacgagtc atccatatca cgctggcagt agccgaagaa tccattcaac agcgggcggt
     2521 gctctatgac aaagaagggg acgcgcattt tgataccatc agcgccttta ttaagagttt
     2581 gcgggggtct gacccggatg ccgcactcta ctggctggcg aaaatggtat atgccggcga
     2641 ggatccccgg tttttgctcc ggcgaatgtt gattctggcc tgtgaagacg ttgggatggc
     2701 cgatccacag gcgctggggg tgataacggc ctgtgcc
//
LOCUS       DAOQDO010000681         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31068_length_2736_cov_3.197687, whole genome shotgun sequence.
ACCESSION   DAOQDO010000681 DAOQDO010000000
VERSION     DAOQDO010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31068_length_2736_cov_3.197687"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKZ53_11510"
     CDS             complement(<1..666)
                     /locus_tag="PKZ53_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HNJ41108.1"
                     /translation="MALKPQILHVHPQVGIPGGEVIVTCQDFDTSNFTQCQAFIGSTR
                     ARIISASTTRVVIGIPEISNAADLSAGIRLKTNQQESNVAAFQVGALLASDLHPVANP
                     AFDPDSGNTYTTLSGARGKKVEVSVWKISSSGKVSKFLSDIINPTGLTFDQEGTLYIS
                     SRYDGNVYRVSPFSESEAFVRDLGVATGIIFNRQGELFVGDRQGIVYRVDELGKAHKF
                     AMLE"
     gene            896..1435
                     /locus_tag="PKZ53_11515"
     CDS             896..1435
                     /locus_tag="PKZ53_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41109.1"
                     /translation="MPLYFTSHHKSTSSVVAAGLALWLSGVGCLLCLMCCWNQGCAGL
                     SDQSCQAETDIVASDATKSESCHSQAESSASHCSASGGGCCASSEESVDDDQGDSLTV
                     ESLVAFNTSQSGCEWCACEGKIELLPASRPSDTPVDQLPAKKAPSVFTLSLKAFSPAP
                     QVYLPNRGHTYLKCCVFLI"
     gene            1545..1898
                     /locus_tag="PKZ53_11520"
     CDS             1545..1898
                     /locus_tag="PKZ53_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41110.1"
                     /translation="MKNIHVIILGFGLVLAGLLYSIPGAAAAENTKAECCKQKEACCA
                     TQQAGCCKAGEQAKAEGCCAAKKECCTATEKPGCCKADAAKKQASANADGSCCFPGSP
                     CCTPGSACCGNKAAA"
     gene            2089..>2736
                     /locus_tag="PKZ53_11525"
     CDS             2089..>2736
                     /locus_tag="PKZ53_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNJ41111.1"
                     /translation="MPKSNRTLLMVLFLTGLLFAQGCHIGRSPSSQESKDKARPTSPV
                     TSAPAQRPSGTGVPASPPAFAAKEASSSMPADADQSNTEDYRDYGVNQMTSAKQDPLS
                     TFAIDVDTASYPIPRRKLNEGQLPPPAAVRVEEFINYFNYNYPQPATAHPFSVTLEAA
                     PSPFQANRHLMRVGIHGKEIPVTNRPPAHLTFLVDTSGSMQAADKIGLLKQSLKLL"
BASE COUNT          680 a          703 c          665 g          688 t
ORIGIN      
        1 ctctaacatc gcaaatttat gtgctttgcc cagttcatca actcgataga caattccctg
       61 gcggtcacca acaaataatt caccctgacg gttaaaaatg attccggtgg cgacacctaa
      121 atcacgaaca aaggcttctg attcgctgaa cggggatacg cgatagacat tgccgtcata
      181 gcggctggaa atatagagcg ttccttcctg atcaaaagtc aggccagtcg gattgatgat
      241 atctgagaga aatttcgaga cttttccact cgaagaaatc ttccataccg aaacttcgac
      301 ttttttgccg cgggcgcctg acagcgtggt ataggtattt ccactatcgg ggtcaaacgc
      361 cggattggca accggatgca ggtcgctggc tagcagggcg ccaacttgaa acgccgccac
      421 attgctttcc tgttgattgg tcttcaatcg gatcccggct gagagatccg ccgcattgct
      481 gatttcagga atgccaatca ccactcgtgt ggttgaggca ctgatgattc gggcccgagt
      541 cgaaccaata aaagcctgac attgggtgaa atttgaggta tcgaaatcct gacaggttac
      601 aatgacttcg ccgccaggaa ttccaacctg tggatgaacg tgtaaaattt gaggtttgag
      661 cgccattcgc tttcgatatc agaatgaaaa tttaaggttg aagagaacgc ctcaccttca
      721 gtgcagaaag gcgtagaaac gcaacatgtt ttgaacctgt tgagcataac acatttgggg
      781 agcaagctgg aacgtcctgc cgagtcccct acctgaggaa aagttcgatc aaacgttcga
      841 actttgctaa aaagtagctg gacattcctc tgattcaaag cgtaagatgc cacctatgcc
      901 tctctatttc acttcacatc acaaatcaac atcgtctgtg gtcgctgccg gattagccct
      961 ctggctgagc ggtgtcggat gtttgttatg tttgatgtgc tgctggaacc aaggctgtgc
     1021 tggactttca gaccagtcgt gtcaggctga gactgacatc gtcgccagtg atgccaccaa
     1081 atcggaatcc tgtcattcac aggctgaatc atcagcatcg cattgttcgg cttcgggcgg
     1141 tggctgttgc gcttcatctg aagaatcggt tgatgatgat caaggagatt ctctcacggt
     1201 cgaatcgctg gtggcattca acacgagtca aagcggttgt gaatggtgtg cctgtgaagg
     1261 aaaaatcgaa ctccttccag cttcgcgtcc atctgataca ccggttgacc agttacctgc
     1321 caaaaaagcc ccatccgttt ttaccctctc gctaaaagcc ttttcgcctg cccctcaggt
     1381 ctatctgcca aaccgagggc atacctacct gaaatgctgt gtcttcctga tttaaatctt
     1441 tgttgatcaa gcacttccgt ccttgtatga ctgagtctgt aaggctcagt gtgtctgaca
     1501 acaaactgta tccgggtttt tacccacaca tcaggagact ttgtatgaaa aacattcacg
     1561 tgatcatttt aggatttggt ctggttctgg ctggtttact ctattcaatt ccaggtgcgg
     1621 cggcagcgga aaacaccaaa gctgagtgct gcaaacaaaa agaagcctgc tgcgctacgc
     1681 aacaggctgg ttgctgcaaa gctggtgagc aggccaaagc ggaaggctgc tgcgctgcga
     1741 agaaagaatg ctgtaccgct acggaaaaac caggttgctg caaagctgat gcggcgaaaa
     1801 aacaagccag tgctaatgcc gatgggagct gctgctttcc tggctcacca tgctgcacgc
     1861 ccggttccgc ttgctgtgga aataaagccg cagcctagtt ggtgaatgtt tggtgcttgg
     1921 tgctttgaat gagtattttc caagccccca agccctaagt tttaatgaag gggtgaatgc
     1981 ctgatgtgtc aggcattcac cctttttcat ttttcaggga acctttcagc ggctgtcgag
     2041 acaatcagca ccatatacca catcaaattt tcggagtgca ttacgtttat gccaaagtca
     2101 aatcgtacgc tgctgatggt cttgtttttg acagggttac tctttgctca aggttgtcac
     2161 atcggacggt caccgtcttc ccaagaaagt aaggataagg ctcgcccgac atcaccggtg
     2221 acctcggcac ctgcccagcg gccatctggc actggggttc cggcatcgcc gccagcattt
     2281 gctgccaaag aagccagctc aagtatgccg gctgacgctg accagtccaa caccgaagat
     2341 tatcgcgact atggcgtgaa ccagatgact tcagcgaaac aggaccctct ctcgacgttt
     2401 gctattgatg ttgacacggc ttcgtacccc atcccccgcc gaaaattgaa tgaaggtcag
     2461 ctcccgccac cggcggcagt gcgcgtcgaa gaattcatta actacttcaa ctataattat
     2521 ccgcaaccag ccacagccca tcctttttca gtgacccttg aagctgcacc ctcaccattt
     2581 caggccaacc ggcatttgat gcgcgtcgga attcacggca aagagattcc ggtgaccaat
     2641 cggcccccgg ctcatttgac ctttctggtt gataccagtg gctcaatgca agcggctgac
     2701 aaaattgggt tgctcaaaca aagcctgaaa ttgctg
//
LOCUS       DAOQDO010000682         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31198_length_2730_cov_2.907290, whole genome shotgun sequence.
ACCESSION   DAOQDO010000682 DAOQDO010000000
VERSION     DAOQDO010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31198_length_2730_cov_2.907290"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKZ53_11530"
     CDS             <1..949
                     /locus_tag="PKZ53_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ41112.1"
                     /translation="VWNHGLFKALLFLAAGSVIHATHTRDIDHLGGLAKRMPNTALCF
                     LVGAVAICGLPPLNGFVSELLIYLGLFKVLISPGSAAWGVATVAIPALALIGALAVAC
                     FVKVYGVVFLGEPRSSACQTGHESSPLMLHPMRVLVGLCVLIGMIPFLVVPLLQTVIQ
                     SWSASVTSTTLSQLAPFEWLSGVGIFLAAGFLLGGWFLARRIPRSIGTPTGTWDCGYA
                     APASTMQYTASSFAQILVSLLSRALLPKIPRLELTDTFPRPVTFHSHVPDTVLDRGLL
                     PTFQRLAQASVRWQALQNGHTQTYIFYIFVTLLILLIMK"
     gene            1086..2006
                     /locus_tag="PKZ53_11535"
     CDS             1086..2006
                     /locus_tag="PKZ53_11535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNJ41113.1"
                     /translation="MLPILLQALIHVIVVLIVPPLLLGMINKTKAFFGGRTGQPLLQG
                     YFDLIKLFQKEMVLSQTTTWVFLAGPVVALVTLVGASLVVPFGHHPAPFSFDGDMVLM
                     AYLLGMGRFFTTSAALDTGSAFEGMGAAREVSFACLAEPALFLGLMALARLTHSISLT
                     GMFANNGIGNWGLAAAPLVLVTASWFLVLLAENSRIPFDDPNTHLELTMIHEVMVLDH
                     SGPAFGLIVYGAAIKLFLFAALVLNLAVPIKPAHPVAGWGLFLVSVGFLAVLIGMVES
                     IMARLRLTNIPKLLVSACLLSAFGVILLIR"
     gene            2006..2656
                     /locus_tag="PKZ53_11540"
     CDS             2006..2656
                     /locus_tag="PKZ53_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNJ41114.1"
                     /translation="MITTDFILNSLLVLVVLLNFPMLGSSRMQAVIQTVALQGFLLGL
                     MPLVGHNQIRWELLIICLLTMAIKGAFIPGLLNRALREVRIQREVAPVLGFTPSILLG
                     GLGTGLAVLFANTLPLAPNQANTLILPASFSTVLTGFLILTTRQKAITQVVGYLTLEN
                     GIFIFGLLLLDAMPFLVETGVLLDLSVGVFVMGIILRYIQRTFSSLEAAPLTTVRE"
BASE COUNT          503 a          670 c          761 g          796 t
ORIGIN      
        1 cgtctggaat cacgggttgt ttaaggcact gctgtttctg gctgccgggt cggtgattca
       61 tgcgacgcat acccgagaca ttgatcacct gggcggattg gcaaaacgga tgccgaacac
      121 cgccctgtgt tttttggtag gagcggtggc aatctgtggc ctgccaccac tcaacggttt
      181 tgtaagcgaa ctgttgattt acctggggct gttcaaagtt ctgatcagcc caggttcagc
      241 ggcctggggg gttgcgaccg tggccattcc agccctggct ttgattggtg cgctggctgt
      301 cgcctgtttt gtcaaagtgt atggcgtcgt ttttctgggt gaaccgcgaa gctcagcctg
      361 tcagacaggc cacgaatcaa gcccgctgat gcttcatcca atgcgtgttc tggtgggtct
      421 gtgtgttctg attggaatga ttccgttcct ggtggttcca cttttacaaa cggtcataca
      481 aagctggtcg gcctcagtca cgtcaaccac gctgtcacaa ctggcaccgt tcgagtggct
      541 gagcggtgtc ggaatctttc tggcggctgg ttttctgctt ggcggctggt ttttggcgcg
      601 gcggattcct cgttcgattg gaaccccaac tggtacgtgg gattgcgggt acgcggcacc
      661 agcttcgacc atgcagtaca ccgcctcatc gtttgctcaa attctggtgt cgctcttgag
      721 tcgggcactt ttgccaaaaa taccacggct ggagcttacg gatacgtttc ccagaccagt
      781 cacttttcat agccatgtgc ctgacaccgt gcttgatcga gggctgttac caacatttca
      841 acggctggct caggcgtctg tccgctggca agcactgcaa aacggacaca cccagactta
      901 cattttttat atttttgtca cactgctaat actgctgatt atgaaatgaa tgaatgacag
      961 tcagtagtca gtcgtcttac cattttgcaa tgagtacttc gtattttgga aagctaaact
     1021 attgaaaaga ttgtatttcc ctcagccctt agccccatac cctcagccca aattggtata
     1081 aacttatgct gccaatactc ttgcaagcct tgatacacgt gattgtggtt ttgattgtgc
     1141 cgccgctcct gttgggcatg atcaataaaa ccaaggcatt ttttggcggg cgaacgggcc
     1201 aaccgttgct tcaagggtac tttgatttga tcaagctttt tcagaaggaa atggtgctca
     1261 gtcaaactac cacctgggtg tttctggccg ggccagtcgt ggcactggtc accctggtgg
     1321 gggcatcact cgtggttcca tttggacacc atccagcgcc gttttccttt gacggagaca
     1381 tggtgttgat ggcctatctg cttggaatgg gacgcttttt cacaacctcc gcagcacttg
     1441 atacagggtc ggcgtttgaa ggtatgggcg cggcgcgtga agtctcgttt gcctgtctgg
     1501 ctgagccggc cctgtttctg gggttgatgg ctctggcgag attaacccac tccatcagcc
     1561 tcaccggtat gtttgccaat aacggaattg gaaactgggg gttggcggcg gcaccgctgg
     1621 ttttggtgac ggcaagctgg tttctggtac tgctggcgga aaattcccgg attcccttcg
     1681 atgatccaaa tacccattta gaactgacca tgatccacga agtcatggtg ctggatcaca
     1741 gcgggccggc gtttggactg attgtgtatg gtgcggcgat caagcttttt ctctttgcgg
     1801 cgctggtgct caacctggcg gtccccatca agccggcgca tccggtcgct ggttggggac
     1861 tctttttggt gtcagtgggt ttcctggctg ttttgatcgg aatggttgaa tccattatgg
     1921 ctcgactccg actgaccaat attcccaaat tgctggtttc tgcctgtctg ctttcggcat
     1981 ttggcgtcat tttgttgatt cgatgatgat tacaacagat tttatcctca attcattgct
     2041 ggtgctggtg gtattgctca actttccgat gcttgggtcc agccggatgc aggccgtgat
     2101 tcaaacggtt gcactccagg ggtttctgct gggattgatg ccactggttg gacataacca
     2161 gattcggtgg gaactgctga tcatctgtct gctcaccatg gccatcaagg gcgcttttat
     2221 tccggggttg ctcaatcggg ctctgcgcga agttcgcatt caacgcgaag tcgccccggt
     2281 acttgggttt acgccttcga ttttgcttgg tggcctggga accggtttgg cggtgttgtt
     2341 tgccaatact ttaccgctcg cccccaatca ggcgaacact ttgattttgc ccgcttcatt
     2401 ttcgacggtt ttgaccgggt ttctgatcct gacaacgcgg caaaaagcta ttactcaggt
     2461 ggtcggttat ctcacgcttg aaaacgggat ttttattttt ggcttgctgc tgctcgatgc
     2521 catgcccttt ctggtggaaa cgggtgtgtt gcttgacctg tcggtgggcg tctttgtgat
     2581 gggcattatt ttgagataca tccagcggac cttttcttct ctggaggccg caccattgac
     2641 gacggtgcgg gagtgaggaa atttagggtt cagggttcag ggttcagggt tcagggctca
     2701 gggttcaggg ttcagggtct tgtcattacc
//
LOCUS       DAOQDO010000683         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31241_length_2728_cov_2.751590, whole genome shotgun sequence.
ACCESSION   DAOQDO010000683 DAOQDO010000000
VERSION     DAOQDO010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31241_length_2728_cov_2.751590"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKZ53_11545"
     CDS             <1..286
                     /locus_tag="PKZ53_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41115.1"
                     /translation="AGNGIEQGNYDVPFRTIRVGDDGIVKTVGTQGALPPGFKPDFDQ
                     QTGRRIFAVGPLANFSITPRTIVTGGVLFAVDGRNYPAGQFWILGVSRVF"
     gene            457..1539
                     /locus_tag="PKZ53_11550"
     CDS             457..1539
                     /locus_tag="PKZ53_11550"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ41116.1"
                     /translation="MSTGLHVLAQTPPPVPATLPPAKIEQIDALFAQWNKPDSPGCAL
                     AVIKDGQIVYKRGYGMADLERNVPISPHSVFDVGSMSKQFTAACILILEQQGKLSVND
                     DIRKYISELPKYDPPVTIRHLLHHTSGIQDYLQLMIRAGMRLENEYSEEDVLKMIQSV
                     PKTEFVPGEQHKYSNSGYFLLSVIVERATGIPLSEFAEQQIFKPLGMTQTHIHDDFTR
                     IVKNRAIGYAPVRSGGFRLEMSLLDTTGDGGVMTTVEDLFLWDQNFYQSKLVSGQDLI
                     TRLITPGVLNSGRKLEYACGIMVGDWKGHQIIHHNGAWAGYRAEMIRFPADRLSVICL
                     ANLGSINPSRLTRQVADLLLTNQGQK"
     gene            1567..2631
                     /gene="rsgA"
                     /locus_tag="PKZ53_11555"
     CDS             1567..2631
                     /gene="rsgA"
                     /locus_tag="PKZ53_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNJ41117.1"
                     /translation="MNKEALGWNESFETSFQPYAQQGMIVGRVYSQDRLGSLIYTDTG
                     EVHAEVSGRLRYLAESRADYPAVGDWVAMTPPTATSQALIEAVLPRKSKFSRKVAGAK
                     TEEQIVVANIDWIFLVSGLDQDFNVRRIERFLTLVRESGAQPLIVLNKADKCADRDQR
                     LREVGAVAICVPIVCISAKHGAGLGALEPFLKKGETISVLGSSGVGKSTLVNRLLGKE
                     LQRTQDVRDGDDRGKHTTRRRELMMLPSGAIMIDTPGMRELQLWATGDGIRETFEDIQ
                     TIAEDCYFRDCRHESEPSCAVLSALQNGTLDPSRFSSYRQLQSEIQLLEVRQNHRAQA
                     VERQRVKRLTQIPKQKRKGW"
BASE COUNT          654 a          682 c          721 g          671 t
ORIGIN      
        1 ggccggaaat ggaattgagc aaggaaatta tgacgtgccg tttcgcacga ttcgggttgg
       61 ggatgatggg attgtcaaaa cggttggtac gcaaggtgcc cttccaccgg ggttcaaacc
      121 ggattttgac cagcaaaccg gacgtcggat ttttgccgtc ggtccgctgg cgaatttttc
      181 aatcacccca cgcacgattg tgactggcgg tgtgttattt gcggttgatg ggcgcaatta
      241 cccggctggt cagttctgga ttttaggagt gagtcgagtg ttttgaacat tttacctttg
      301 acattcgtac ccgcgttttc caccacaaaa tgtcaaaggt atctcctcaa tctctcgggg
      361 ctcgatacct gattcctcca ggcacaaaag gaccatttca tgaccgccgc tttcgttcgg
      421 gtacgagccc tgctggctct ttgcattgtt ttctgtttgt cgactggact tcacgtcctg
      481 gcccagacgc caccgccagt tcctgccacc ctcccaccag ccaaaatcga gcaaattgac
      541 gccttgtttg cccagtggaa caagcctgat tcgccaggat gtgcgctggc tgtgatcaag
      601 gatggtcaaa ttgtctacaa acgcggctat ggcatggctg atctggaacg caatgtcccc
      661 atttcaccac acagcgtgtt tgacgttggt tcgatgtcaa aacaatttac cgccgcctgt
      721 attttgattt tggaacagca agggaagctc tccgtcaacg atgacattcg aaaatacatc
      781 tccgaactcc ccaaatacga cccacctgtc acgatccggc atctcctcca ccacaccagc
      841 ggtattcagg actatctcca gcttatgatc cgggcgggga tgcggttgga aaatgaatat
      901 tccgaagaag atgtcctgaa aatgattcaa tcggttccca aaaccgagtt cgttccgggt
      961 gagcagcaca aatacagcaa ttccggctat ttccttcttt cagtgattgt tgagcgtgca
     1021 acgggtattc cgctcagtga atttgccgag cagcaaatct tcaaaccatt aggaatgaca
     1081 caaacccaca ttcatgacga ctttacccgg attgtcaaaa accgggcgat tgggtatgct
     1141 ccggttcggt ctggaggatt tcgacttgaa atgtcgttgt tggataccac aggtgatggt
     1201 ggtgtgatga caacggtaga agatttattc ctctgggatc aaaattttta tcagagtaaa
     1261 ctggtgagtg gtcaggatct gattacgcgt ctgattacgc caggtgtttt gaactccggt
     1321 cgaaaactag agtatgcttg tggcattatg gttggtgact ggaaggggca tcagattatt
     1381 catcacaacg gcgcctgggc tgggtatcga gcggagatga ttcgatttcc cgctgatcgg
     1441 ctgtcagtga tctgtctggc aaaccttggg tcaatcaatc catcacggct gacacgtcag
     1501 gtggcagacc ttttgcttac aaaccagggg caaaagtaag tttcgaggcg catgagggaa
     1561 gtgaacttga acaaagaagc acttggttgg aatgaatctt ttgaaaccag ctttcagccc
     1621 tatgcccagc aagggatgat cgttgggcgg gtctattccc aggaccgact tggttcgctg
     1681 atttatactg acactggcga agtgcacgcc gaagtcagtg gtcggttgcg atatctggcc
     1741 gaaagccggg ccgattatcc agcagttggt gactgggtgg ccatgacgcc gccaaccgcg
     1801 acgtcacagg ccctcattga agctgtcctg ccgcgcaaaa gtaaattctc ccgcaaagtc
     1861 gctggtgcca aaaccgaaga acaaattgtc gtcgccaaca ttgactggat ctttttggtt
     1921 tcaggattgg accaggattt caatgtgcgt cggattgaac gatttctgac actggttcgg
     1981 gaaagtggtg cccagccgct gattgtcctc aacaaagccg ataaatgtgc tgaccgtgac
     2041 cagcgcctgc gtgaagttgg cgccgtggcc atctgtgttc caattgtgtg tatttccgcc
     2101 aaacatggcg ctggactcgg cgcgcttgag ccctttctga agaaaggcga aacgatttcg
     2161 gtgttagggt cctctggggt cggcaaatct actctggtca accgcctgct tggaaaagag
     2221 cttcaacgaa cccaggatgt ccgcgatggt gacgaccgtg gcaaacacac cacccgccgc
     2281 cgtgaattga tgatgcttcc ttcgggggca attatgattg acacgcctgg catgcgtgaa
     2341 ttgcagttgt gggccacagg tgacggcatc cgcgagacct tcgaagatat ccagacgatt
     2401 gccgaagatt gctatttccg tgattgtcgc cacgaaagcg aaccctcatg tgccgtgctg
     2461 agcgcgctcc aaaatggaac tcttgatccc agccgttttt ccagttatcg ccagctccaa
     2521 agtgaaattc aactcctcga agttcgccaa aaccaccggg cgcaggcggt cgaacgccag
     2581 cgcgtcaaac gactgaccca aattccaaaa caaaagcgca aaggctggta ggaagaatgt
     2641 gcttggtgct tggtgcttgg tgcttggtga ctgggaatca atgctttcga agtagaacca
     2701 agaaccaaga accaagaacc aagaacca
//
LOCUS       DAOQDO010000684         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31246_length_2728_cov_2.265619, whole genome shotgun sequence.
ACCESSION   DAOQDO010000684 DAOQDO010000000
VERSION     DAOQDO010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31246_length_2728_cov_2.265619"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2584)
                     /locus_tag="PKZ53_11560"
     CDS             complement(<1..2584)
                     /locus_tag="PKZ53_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ41118.1"
                     /translation="MQKNTVLQLKCRLIKLLCLLPWNTVKRVISALLWSVAFSHLVLA
                     QAGKAELTGIVFDSQSVPVAGVRIWLTEQQTQQMVEFTTDSTGTFTFPALRPGRYLAR
                     FETQGFKPVIQGPFRLGTGERVRLDVKLEPGTVEELIIVEGDAMLLRTESATVGQVIS
                     ERTIKELPLNGRNFISLATLAPGVALPPGSFFPRINGGRPRTNEYLFDGISVLQPEPG
                     QVVFFPVVDAIQEFKIESNTPSAEFGRFNGGVVNLTTKSGSNELHGTGFEFFRNEALN
                     ARTLFAPKTAANPNKPLFRRHQFGFVLGGAIIKNRTFFFTDYQGTQQAIGRVLISTVP
                     TPLQRQGVFTESIAGIMPRIYDPATTRPKTGGGFERDPFPGNQIPGNRLDPVAVALLN
                     RYPLPNLAGTANNFRRIGTESQDQNQFDIRLDHQVSPKDQLFGRYSLAKDTVRPVTPL
                     PEGSGSITLGAIAPTQTTGQSFAASYIHLFNDRMVNEVRVGYTRRAIDRTALLLDAPP
                     SQSLGLPGIPSNGAFETELPTFVIDGFQQLGPPANTNSDFRTDVTQVVDIFSLQRGRH
                     SLKLGFDGRWSRLDVTQPPSPTGNFRFSSLFTDLPGTAGTGFSWASFLLGQVQTFSID
                     LQEKVLRPRAHTLEFFAQDDWKVTNRLTLTGGLRYTLNFPSTEAENQGAVFNLTTQQL
                     DYLGQNGVSQSARRLHKLNFGPRVGLAYRIGEKTAIRAGYGLTWIEQTGITTPFTIPQ
                     FPFIQTVSERSLDSINPAFVLSTGPRAAPLPLSPDAGLGQGVFSVERELGSGYVQQWN
                     LAIQREIGHNWTVEVAYVGSKITRLGIPDTNLNQLTVDQLALGSKLLERVPNPFFGQI
                     PRSSS"
BASE COUNT          680 a          701 c          753 g          594 t
ORIGIN      
        1 gagatgacga ccgtggaatt tgtccaaaga agggatttgg cacccgttcc agtaactttg
       61 acccaagcgc cagttggtcc acggttaatt gattgagatt ggtgtcggga atgcccagcc
      121 gtgtgatttt cgacccgacg taggcaactt caaccgtcca gttgtgacca atttcccgct
      181 ggatggccag gttccactgc tgcacgtacc cggagccaag ttctcgctcg actgaaaaca
      241 cgccctgacc cagtccggca tccggagaaa gtggcaacgg ggcggctcgc ggaccagttg
      301 acagaacaaa cgccgggttg atgctatcga gcgaccgctc agaaacagtt tgaataaaag
      361 gaaattgcgg aattgtgaac ggcgtcgtga tgccggtctg ctcaatccag gtcagcccat
      421 atccggcccg aatggcggtt ttttcgccaa tccgataggc aaggccgacg cgtggcccaa
      481 agtttaattt gtgcaggcga cgggcgctct gcgaaacacc attttgccca aggtagtcaa
      541 gctgttgggt tgtgaggtta aatactgcgc cctgattctc agcttcggtc gaaggaaaat
      601 tcagtgtgta ccgaagcccg cccgtgagcg tcaaccggtt ggtcactttc cagtcatcct
      661 gggcaaagaa ttccagcgtg tgcgctcgtg gccgtaaaac cttttcctgc aaatcaatcg
      721 agaaggtctg aacttgccca agcaaaaaac tggcccacga aaagccggtt cccgccgttc
      781 ccggcaaatc ggtgaataag ctgctaaatc gaaagtttcc ggtcggcgat ggcggctggg
      841 tcacatccag tcgcgaccag cgtccatcaa aaccaagttt caacgaatgc cttccacgtt
      901 gaagcgaaaa gatatccacc acctgggtga cgtcggttcg aaaatctgaa ttggtgttgg
      961 ccggcggccc cagttgctga aacccatcaa tcacaaaggt tgggagttcg gtttcaaaag
     1021 caccatttga gggaatcccc ggcaaaccaa ggctttgcga cggtggggca tctaaaagta
     1081 acgcggtgcg gtcaatcgcc cgacgggtgt acccgacgcg gacttcattc accatccggt
     1141 cattgaacaa atgaatataa ctcgcggcaa atgattgccc ggtcgtttgg gtcggggcaa
     1201 ttgctcccaa agtgatactc ccactgcctt ccggcaatgg cgtcaccggg cgaacggtat
     1261 ccttagccag cgaatagcgt ccaaagagtt gatctttggg tgaaacctga tggtcaagcc
     1321 ggatgtcaaa ctgattctgg tcctgtgact cagtgccgat tcgccggaaa ttgttggccg
     1381 ttccagcaag atttggcaac gggtagcggt tcaaaagcgc aacagccacc gggtccaggc
     1441 gattacctgg aatttgattg ccgggaaatg gatcgcgttc aaaaccgccg ccagttttgg
     1501 gtcgagtggt ggccgggtcg taaatgcgcg gcataatccc cgcaattgat tcagtaaaaa
     1561 ctccctgtct ctgtaacggt gtcggtaccg ttgagatcaa cacccggcca atcgcctgct
     1621 gtgttccctg gtaatcggta aaaaagaacg tgcggttttt gataatcgct ccgccaagga
     1681 caaagccaaa ttgatggcgg cgaaagagcg gcttgtttgg gttggcagca gtttttggag
     1741 caaacagggt acgggcgttg agtgcttcgt tgcggaaaaa ttcaaagccg gtgccgtgaa
     1801 gctcgttgga acctgatttg gtcgtcaaat tgaccacgcc accattaaac cggccaaatt
     1861 cagccgacgg tgtgttgctt tcaattttga attcctggat ggcatcaacc actggaaaga
     1921 acacaacctg tcctggttcg ggctggagta cggaaatgcc atcaaacaag tattcgttgg
     1981 ttcggggccg tccgccgttg atgcgtggga aaaatgaccc aggcgggagc gccacacctg
     2041 gtgccagggt cgccagtgaa atgaaattgc gtccgttgag tggtaattcc ttgatggttc
     2101 gctcagaaat tacctgcccg acagtggcag attcggtccg aagcaacatg gcgtcacctt
     2161 cgacaatgat gagttcttcg acggttccag gttcgagttt gacatccaat cgaacccgtt
     2221 ccccggtccc taaccgaaat ggcccctgga tcaccggttt aaagccctgc gtttcaaagc
     2281 gtgccagata ccggcctggt ctcagtgccg gaaaagtaaa cgttccagtc gagtcagttg
     2341 taaattcaac catttgctgg gtttgctgct cagtcaacca aatgcgcaca cccgccaccg
     2401 gtaccgattg agaatcaaaa acgatgccgg ttaattctgc ttttccagcc tgggccagta
     2461 ccaggtgact gaaggcgacc gaccacaaca gggcgctgat gacccgtttt acggtgttcc
     2521 aaggcaggag acacagtaac ttgatcaggc gacattttaa ttgaaggaca gtgttttttt
     2581 gcataaatca taccattgag ggtcccgagg tagtgaaaag gacgaaagag acggaaaaga
     2641 cggaaaggac gaaagggacg gaaaggacga aagggacata aattcgaaga cccggaaccc
     2701 ggaacccgga acccggaacc cggaaccc
//
LOCUS       DAOQDO010000685         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31305_length_2725_cov_2.982772, whole genome shotgun sequence.
ACCESSION   DAOQDO010000685 DAOQDO010000000
VERSION     DAOQDO010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31305_length_2725_cov_2.982772"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..991
                     /locus_tag="PKZ53_11565"
     CDS             440..991
                     /locus_tag="PKZ53_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41119.1"
                     /translation="MILKITSTSKVRTLLTFGLLDALFVWCLFLIPVLVVAFLSGRHV
                     LSDLPRFLEMYWAIGAPLMVPLVVFGAWRGAFDAVRTKFGTPSLTRVTAEGFVLGFCY
                     CLGACAFGLFQAVYAAGTIYDEVAAHPTHPWAWIGVLGIMLPFCIASGIPGSCFGALI
                     HIFNQTVLRGLDGIFHRKIEATL"
     gene            954..1460
                     /locus_tag="PKZ53_11570"
     CDS             954..1460
                     /locus_tag="PKZ53_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41120.1"
                     /translation="MGYSIEKLKRPYDYKVLVEAYVRTQRKDYVHPDFSDYYQSLCAA
                     LEKHFEICFSPTHLSGSNRVLWILFDSTMRSLLRITTPWDGFLEETLIVSTLQKSGDA
                     GKRIDQASQTIWQATRESRQAHLEMLHELFALMFGKTNMIVTSEQLALAGFDDSQEPD
                     INDYFDYF"
     gene            1583..2236
                     /locus_tag="PKZ53_11575"
     CDS             1583..2236
                     /locus_tag="PKZ53_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HNJ41121.1"
                     /translation="MPHYLDISQWARRDLFEFFREYDNPYFNICTRLDITKLKPTVAQ
                     VPEATIFLGYQYITLRLVNEIESFRYRLEDGKVRVYDVIHGGTTVLMEDGSFTFAYFD
                     YDVRFSKFVTGAREELARVKAGDGRLHPKHEDNARIHSTTLPWISFTSFTNARKWRQE
                     DSIPKFAFGRFTKEGDRVWLPISIEVHHALMDGYHVGQYLNRFEEILEDPASVLLEG"
     gene            2427..>2725
                     /locus_tag="PKZ53_11580"
     CDS             2427..>2725
                     /locus_tag="PKZ53_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ41122.1"
                     /translation="MTFSTNSTSLDLTSTILRLGVDTGGTFTDFVLLEGATLRVHKVS
                     STPDDPSRAILQGVVDLLGKTSQTCPVVDLVHGSTVATNALLERKGAAVALITTAG"
BASE COUNT          650 a          652 c          688 g          735 t
ORIGIN      
        1 cagtccgcac tacgcggttt ggctgcacca atgacaccac gaccaatctt tccaaagaag
       61 ccttccagct tctgaagaaa aaatacccca ccgacccctg ggcacagaag acgaagtacc
      121 attattaggg ctgaagactt cgggctgaaa acttcgggct gaagactttg ggcttggggt
      181 ttttgaaggg tgaaggatga gcaattgtcc tttattaggg cttggggctt agggctgagg
      241 gcttggggtt tttgaagggt gaaggatgag caactgtcct ttattagggc ttggggctga
      301 gggctgaggg cttggggttt ttgaagggtg aaggatgagc aactgtcctt catccttttg
      361 cccaaattga gatttgagga gggcgattga ccctggagtt atttgcccct gtagctaaag
      421 gtgattggag ggtttggtca tgatcttaaa aattacctca acttcaaaag ttcgaactct
      481 tctgacattt ggtctgctgg atgccctttt tgtatggtgt ctttttctga ttccagtttt
      541 ggtagtggcg ttcctgagtg ggcggcacgt tttaagcgat cttcctcgct ttcttgagat
      601 gtactgggca attggcgctc cactgatggt gccgttggtg gtgtttggtg cctggcgtgg
      661 tgcctttgac gcagttcgga caaagtttgg aactccatca ctcacacgag tcacggctga
      721 aggatttgtc ttgggattct gttattgcct cggggcgtgt gcctttggat tgttccaggc
      781 agtgtacgca gccgggacga tttatgacga ggtggcagct caccccactc atccctgggc
      841 ctggattgga gttcttggaa ttatgctgcc cttttgtatc gcaagcggaa tccctggaag
      901 ttgtttcggc gccctgatcc acatttttaa tcagacagtt ttgagaggat tggatgggat
      961 attccatcga aaaattgaag cgaccctatg actacaaagt gctggtggag gcgtatgtcc
     1021 ggactcagcg gaaagattat gttcacccgg actttagtga ctattaccaa agcctttgtg
     1081 ctgccttgga aaagcacttt gagatttgct tttcacccac acatctctct ggttccaacc
     1141 gggtgctctg gatattgttt gactcaacga tgagatcttt gctccgaatt acaactccgt
     1201 gggatggatt tcttgaagaa accctgattg tctccacatt acaaaaaagc ggagatgctg
     1261 ggaaaaggat agatcaagcc agtcaaacca tctggcaagc aaccagagag agtcggcagg
     1321 ctcatttgga gatgctgcat gaactgttcg ccctgatgtt tggcaaaacc aatatgattg
     1381 tcacgtctga gcaacttgcg ctggccgggt ttgatgattc acaagaacct gacatcaacg
     1441 actattttga ttatttttaa gcctgtcact tctagcttca aagaacactg gcatactcct
     1501 gaaaccctga accctgtttt tcaaaccctg aaccccgaac cctgaaccct gtttttcaaa
     1561 ccctgaaccc tgtttttccc ctatgcccca ctatctcgac atttcccaat gggcccggcg
     1621 cgatttattt gaattttttc gcgaatatga caacccgtac tttaatatct gcacgcggct
     1681 tgacattacc aaactcaaac ccacggttgc ccaggtacct gaggcaacga tttttctcgg
     1741 gtatcaatac atcacgctcc ggctggttaa cgaaatcgaa tcgtttcgct atcggctcga
     1801 agatggcaaa gttcgtgtgt atgacgtgat ccacggcgga accaccgtgc tgatggaaga
     1861 cgggagcttt acgtttgctt actttgacta tgatgtgcgg ttttcgaagt ttgtgactgg
     1921 tgcacgtgaa gaactcgccc gcgtcaaagc cggtgatggc aggctgcatc ccaaacacga
     1981 agacaacgcc cggattcact cgacgaccct gccctggatt tccttcacca gtttcaccaa
     2041 tgcccgcaaa tggcgacagg aagattcgat tccgaaattt gcctttggcc gctttaccaa
     2101 agaaggcgac cgggtgtggc ttccgatttc tatcgaagtt caccatgcgc tgatggatgg
     2161 ctaccacgtt ggccaatacc tcaaccggtt tgaagaaatt ctggaagacc cagccagtgt
     2221 gctgcttgaa ggatgaagaa ccaggaaaaa tatcaggttt tactgatatt tggggtagtg
     2281 tatcgagtta ccgtaatgga atcgtaactg gtgcctcttc aaccaccact tctgcagcaa
     2341 agaactatca accgaacctg atatgttgaa ccagaaccag gccagtccta cgatagaaaa
     2401 agcaaaaacc aaaacggaat gtgatcatga ccttttcaac gaactccacc tcacttgatc
     2461 taacttccac catacttcga cttggcgtag acaccggcgg cacatttacc gattttgttt
     2521 tgcttgaggg agcaacactt cgagttcata aagtcagttc gaccccagat gacccgtcgc
     2581 gggcaattct tcaaggggtc gttgacctgc tcgggaaaac gtctcaaacc tgcccggtgg
     2641 ttgatctggt gcatgggtca accgtggcca ccaatgcctt gttggaacgt aagggggctg
     2701 ctgtcgcact catcacgaca gcagg
//
LOCUS       DAOQDO010000686         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31327_length_2724_cov_3.054702, whole genome shotgun sequence.
ACCESSION   DAOQDO010000686 DAOQDO010000000
VERSION     DAOQDO010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31327_length_2724_cov_3.054702"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PKZ53_11585"
     CDS             <1..1483
                     /locus_tag="PKZ53_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41123.1"
                     /translation="TLTVYRCRFVDPTVPASQTQTSSTPQSVEPPVSFPEIQTRLRTF
                     TDTSLQEHPASTVTNQFGTASFWVRREGQYVLAVLRITPASKFKKVVLTTPSWTLGET
                     TVQPKFTQQPLPETMKKASVTGSFLFDLSESSEALQTTTGSSQPVLGVQMGVQTALIS
                     LQASPASVVDQTGPVGTDAKAFPMLSSPASESQREPSVGIPQRSDSPLTPAQQDQVQR
                     LNQITSGTTGSISPVAYRSDESLPAERQSFSLAQERLDFLTQTIGIRSDQVKRVTVGE
                     VLAGIYTEQFQGITLAIVCLERFRGSTPREISPLQWDTGSQVVTARAIFNPFPRTLTT
                     VPLISGFIFDAPSISSDRFKCVFKVGNDSVKIQAGSELTQGSTDSTEKIFQAGSVEFS
                     VSRLRLENIGPILRVKARNPGTQPIEKIELLVVTAGKASSGTQLAQLTQLDPIQSQIE
                     PVSLRGTRSIDFRIPESLQPDQLEIRWAHQGTWTSVRVSFHSK"
     gene            1561..>2724
                     /locus_tag="PKZ53_11590"
     CDS             1561..>2724
                     /locus_tag="PKZ53_11590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41124.1"
                     /translation="MKRAGKYELLEQIGTGGMGTVWKAFHPRFEKYVAVKEILAHSAN
                     DQSVKQRFEHERQVLTQLPEHHHLVSVLDAFEWQGRYFVVMDYIEGHTLREIICLGPT
                     SPETAVELFKPVLTGLLALHQAGFVHRDLKAENVMVGLDGSVVIIDFGLAESITSPPQ
                     NQLGTAKYTAPELIDPQKNRGADRVQADLYAAGILAYELIVGEKRFRDTFRQIYRGSS
                     DSLNQRWLEWHCNFNTEAPVLHEIDPHIPVFLSRTVESLMAKHTNNRFRSASDALRAL
                     GHRPGPEQNTTTHSRSARNEPSRQRVTADQNVRIGSLRSTTFHSVSPTLSRPSKSRFF
                     SSAPQWLVWAVGAVWITLVTICIGLIFLADPVGFTVTVQGAPPGSTVLVDNTPR"
BASE COUNT          693 a          736 c          651 g          644 t
ORIGIN      
        1 aacactcacc gtatatcggt gtcgctttgt ggatccgacc gtcccagcca gccagaccca
       61 gaccagttca actcctcaat ccgttgaacc acccgtgagc tttcccgaga tccagactcg
      121 attgcggaca tttactgaca cgtcacttca ggaacatcca gccagtaccg tgaccaatca
      181 atttgggacg gcttcatttt gggttcgtcg cgaaggccag tatgtgctgg cagttttgcg
      241 gataactcca gcgtcaaaat tcaaaaaagt tgttttgacc actccaagct ggacgctcgg
      301 agaaaccaca gtccaaccaa agttcaccca acaacccctg ccggaaacaa tgaaaaaagc
      361 atcggtgaca ggaagctttc tgtttgatct gagtgagtca tccgaagccc tccagaccac
      421 cactggttct tcacagccag tcctcggggt tcaaatgggc gtgcaaacgg ccctgatttc
      481 actccaggca tcacccgcct ctgtggttga ccaaactgga cctgtgggaa cagacgccaa
      541 agcgtttccg atgctttctt caccagccag tgaatcacaa agggaaccat cggtgggaat
      601 tccacaaagg agtgactctc cgctgacccc agctcaacaa gatcaggtgc aacgactcaa
      661 tcaaattacc agtggcacaa cgggttcaat ttccccagtt gcgtaccggt ccgacgagtc
      721 tctccccgct gagcgccagt ctttttcgct ggcccaggag cgtctcgatt ttttaactca
      781 aacgattggg attcgctcag atcaggtcaa acgggtgact gtgggggaag ttctggcagg
      841 tatttacacc gagcaatttc agggaataac actggcgatt gtctgcctgg aacgttttcg
      901 aggaagcaca ccacgtgaaa tctcacctct gcaatgggat accggtagtc aagtggtaac
      961 agctcgtgcc atcttcaacc catttcctcg aacactcacg acggtaccat tgatctctgg
     1021 attcattttt gatgcaccgt ccatttcatc tgaccgcttc aagtgtgtgt ttaaggttgg
     1081 aaatgacagt gtcaaaatcc aggctggatc ggaactcacc cagggcagca cagacagcac
     1141 agaaaaaata tttcaagctg gctcagttga attttcagtg agtcggcttc gtctggaaaa
     1201 catcggcccg attttacgag tgaaagcccg gaaccctggc actcaaccga ttgaaaaaat
     1261 tgaacttctg gttgtcactg ccggcaaggc ttcatctgga acgcaactgg cccaattaac
     1321 ccagcttgac ccgatacaaa gtcaaattga accggtgagc ctgcggggaa cgcgatcaat
     1381 tgattttcgc attcctgaat cgctccagcc tgaccagctt gaaattcgct gggcacatca
     1441 gggtacctgg accagtgtca gagtttcatt ccattccaaa tagcatcaac cacactcttt
     1501 ttttcaagga gttcaaccag ttcctccttt gacaacccct ctcacactgg gacaggtaat
     1561 atgaaacgag ctgggaaata tgaattactg gaacaaatcg gcactggcgg aatgggaacg
     1621 gtttggaaag cgttccaccc cagatttgaa aagtatgtcg cggtgaaaga aattctggca
     1681 cacagtgcca atgaccagag tgtcaaacag cggtttgaac atgagcggca agtcttaacg
     1741 caactccccg agcaccatca cctggtgtcg gtgctcgatg cctttgaatg gcagggacgc
     1801 tattttgtgg tgatggacta tattgagggt cataccctgc gagaaatcat ctgcctggga
     1861 ccaaccagcc ctgaaaccgc cgttgaatta ttcaaacccg ttttaaccgg gttactggca
     1921 cttcatcagg caggatttgt ccatcgcgat ttaaaagccg aaaacgtcat ggttggtctc
     1981 gatgggagcg tggtgatcat tgattttgga ctggctgaat caatcaccag tcccccgcaa
     2041 aaccagcttg ggacagccaa atacacggca ccagaattaa ttgatcctca gaaaaaccgt
     2101 ggggcagatc gggttcaagc tgatttatat gcggctggga ttctggctta tgaattgatc
     2161 gtaggcgaga aacggtttcg agatacattc cgccagatct atcgcggaag ttccgacagt
     2221 ctcaatcagc gatggctgga atggcactgt aatttcaaca ccgaagcgcc ggtcctccac
     2281 gaaattgacc cacacatacc agtatttctt tccagaacgg ttgagagtct tatggcgaaa
     2341 cacaccaaca atcgttttcg atccgccagt gatgccttgc gtgcgcttgg gcaccgtcca
     2401 gggcctgagc aaaatacaac cactcattca agatctgccc gtaatgaacc ctccaggcag
     2461 cgggtcacgg ctgatcaaaa tgtcaggatt ggttcacttc gatcaaccac atttcactct
     2521 gtttcaccaa ctctgtcacg cccctccaaa tcaaggttct tttcctctgc cccgcaatgg
     2581 ctggtgtggg ccgttggggc tgtttggatc acgctggtca ccatttgcat cgggctgatt
     2641 ttcctggccg acccggtggg ctttactgta acggttcagg gcgcaccgcc tggaagcacc
     2701 gtgctggtag ataatacccc gcgc
//
LOCUS       DAOQDO010000687         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31359_length_2723_cov_2.413418, whole genome shotgun sequence.
ACCESSION   DAOQDO010000687 DAOQDO010000000
VERSION     DAOQDO010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31359_length_2723_cov_2.413418"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..>2723)
                     /locus_tag="PKZ53_11595"
     CDS             complement(178..>2723)
                     /locus_tag="PKZ53_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ41125.1"
                     /translation="GQELCHLVKSIPEDHDLVIDFYLWNPDRSQSADELQQDIQRHLN
                     EVPFPRPIRRIVVSVASLATGHEMEGTWHFTFRPSPEGYQEEKIYRSLHPMMGKRLHL
                     WRLSNFNLERLPSVEDVYLFRVLAKDNPKDERLIAIAEVRDLTIVRDGNGQIIQIPYL
                     EHMLFEAAAGLRHFLAQRPPRERPQWNRLLLYIWPTLTLNREEINQVSRKLSAATEGL
                     GLERMVIRCTLPDAETGEIKDTVWRISNPGGHEPVVSFHTPAEKPMRPLGDYERKVLQ
                     LRRRGLVYPYEIVEMLTSSQGEKIQFPPGEFQEYDLNEQNELVPVNRPYGLNKANLVV
                     GVITNKTAKYPEGMTRVIMLGDPSKSMGSVAELECRRILACIDLAQKMNVPLEWFPVS
                     AGAKISMESGTENMDWIADVLRALIEFTQAGCEVNIVVNGINVGAQPYWNAEATMLMH
                     TRGILIMTPDGAMVLTGKRALDFSGSVSAEDNFGIGGYDRVMGPNGQAQYWAADIVEA
                     SEILLRHYDHTYVVPGERFPRDTPSTDPTDRDIRSFPYSGNKADGFELVGEIFTEEKN
                     AGRKRPFDIRSVMRAVVDQDHQPLERWFGFRDAEIPVVWDAHLGGHPVCMIGMESRPL
                     PRLGFISADGPYQWTAGTLFPQGSKKVARAVNSASGNRPLVVLANLSGFDGSPESMRK
                     TQLEFGAEIGRAVVNFKGPIVFCVVSRYHGGAYVVFSKTLNKNLEVAALEGSFASVIG
                     GAPAAAVVFAGEVEKRTKADDRLTTLEKAIAAAAGPDKGRLRAEYTDLYNVVYSEKLG
                     QVAEEFDTVHSVHRALAVGSLDRIVPSSQLRPYLIDAVERGMKKALESR"
BASE COUNT          568 a          796 c          754 g          605 t
ORIGIN      
        1 aacccggaac ccggaacccg gaacccgaaa cccgaaaccc ggaacccgga acccgaaacc
       61 cgaaacccga aacccggaac ccggaacccg aaacccgaaa cccggaaccc ggaaccctaa
      121 accctaaacc cggaacccta aaccctaaac ccggaaccct aaacccggaa cccggtctta
      181 cctgctttcc aacgctttct tcattccgcg ttcgaccgca tcaatcagat atggacgaag
      241 ctggcttgac ggaacgatcc gatccagcga cccgaccgcc agtgcccggt ggacactgtg
      301 gacggtgtcg aattcttcgg ccacctggcc gagtttttcg gaatagacca cgttgtagag
      361 gtcggtgtat tcggcccgca ggcggccttt gtctggaccc gccgctgccg caatcgcttt
      421 ttcaagcgtc gtcagccggt cgtcggcttt ggttcgtttt tcaacttcac cggcaaacac
      481 gacggcggct gccggagcgc ctccgatgac cgaagcaaac gagccttcga gtgcggcgac
      541 ttcgaggttt ttgttcaagg tctttgaaaa gaccacatac gccccgccgt gatagcgcga
      601 caccacgcag aacacaatcg gtcccttgaa gttcacgacc gcccggccaa tttcagctcc
      661 gaattcaagc tgggtcttgc gcatggattc cggtgagccg tcaaacccgg acaggttcgc
      721 cagcaccacc agtggccgat tgccgctggc cgaattgacg gctcgggcga ctttcttgga
      781 gccttgtggg aagagcgtcc ctgccgtcca ctgataaggt ccatccgctg agataaagcc
      841 gagccgtggc aatggacgtg attccatgcc gatcatacac acgggatgtc cgccaaggtg
      901 ggcatcccag accaccggaa tttccgcatc ccggaaccca aaccagcgtt caagcggctg
      961 gtggtcctga tcaaccaccg cccgcatcac ggaccgaata tcaaatggcc gtttgcgtcc
     1021 ggcgttcttt tcttcggtaa agatttctcc gaccagttcg aagccgtccg ctttgttgcc
     1081 ggaatatggg aagctgcgga tgtcgcgatc cgttgggtcg gttgatggtg tgtcacgtgg
     1141 aaatcgttcg ccgggaacga cataggtatg gtcataatgc cgaagcagga tttctgaggc
     1201 ttcgacgata tccgccgccc agtattgggc ctgtccgttg ggacccatca cccgatcata
     1261 gccgccgatg ccgaagttgt cttctgccga aacgctcccc gaaaaatcga gggcgcgttt
     1321 gccggtgagg accatcgctc cgtcaggcgt cataatcagg atgccacggg tgtgcatcag
     1381 catggtggcc tcggcgttcc agtacggctg ggcgccgacg ttgatgccgt tgaccacgat
     1441 gttgacttcg cacccggcct gggtgaattc gatcagggcc cgcagcacgt ccgcgatcca
     1501 gtccatgttt tccgtgccgc tttccattga aattttcgcc ccagccgaga ccgggaacca
     1561 ttcgagcgga acgttcattt tttgggccag atcaatgcag gccagaatcc gccggcattc
     1621 gagttcggca actgagccca tgctcttgct tgggtcgccc agcataatca cgcgggtcat
     1681 gccttcggga tatttggcgg ttttgttggt aatcacgccg acgaccagat tggctttgtt
     1741 caagccgtat ggccggttga ctggaaccag ttcattctgt tcgttgaggt catattcttg
     1801 aaattcgccg ggcgggaact gaattttctc gccttgcgat gaggtgagca tttcgacgat
     1861 ttcatagggg tacaccagcc cacggcggcg aagttgcagt actttgcgtt cataatcgcc
     1921 cagcggacgc atcggttttt cagccggcgt gtggaatgaa accaccggct cgtgtccgcc
     1981 agggttggaa atccgccaca cggtgtcttt gatttcgcca gtttccgcat ctggcagggt
     2041 acagcggatc accattcgtt cgagtcccag cccttcggtt gccgctgaaa gtttgcgcga
     2101 gacctgattg atctcctcgc ggttcaaggt cagcgttggc cagatataca ggagcaatcg
     2161 gttccactgt ggtcgttctc gcggaggccg ttgcgccagg aaatgccgca atccggcagc
     2221 cgcttcaaag agcatatgtt ccagatatgg aatctggata atctggccat ttccatcacg
     2281 cacaatggtc aggtcgcgga cttcggcaat cgcaatcagc cgttcgtctt ttggattgtc
     2341 tttggccagc acccggaaga gatagacgtc ttcaaccgag ggcaggcgtt cgaggttgaa
     2401 attgctcaac cgccagagat gcaaccgttt acccatcatt gggtggaggc tgcgatagat
     2461 tttttcttcc tgatagcctt ccggtgatgg ccggaaggta aaatgccagg tgccttccat
     2521 ttcgtgtccg gttgccaggc tggcgaccga aaccacaatt cgccgaattg gacgcgggaa
     2581 gggcacttcg ttcaaatggc gctggatatc ctgttggagt tcatctgccg actgactacg
     2641 gtctggattc cagaggtaaa aatcaatcac caggtcgtga tcttccggga tagatttgac
     2701 caggtggcac agttcctgac cgg
//
LOCUS       DAOQDO010000688         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31377_length_2722_cov_2.915261, whole genome shotgun sequence.
ACCESSION   DAOQDO010000688 DAOQDO010000000
VERSION     DAOQDO010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31377_length_2722_cov_2.915261"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKZ53_11600"
     CDS             <1..692
                     /locus_tag="PKZ53_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ41126.1"
                     /translation="PYQTSSDWQAEAFAPGDGLKLPESLNRSQIPPTDVLDTLFQLLP
                     ENAIVTTDVGQHQMWTAQRARPIDPRNFITSGGLGTMGFGVPSAIGAQFACPDRLVVA
                     VVGDGGFQMSIAELATLKRHKLPVKVLVMDNKYLGMVRQWQELFYQGRYSAVDLRDNP
                     DFAAIAVAYGLTSFKLTEPEAVESTLREWLATPGPALLHCECHIAENVFPMIPAGAAI
                     DEMMEATPTRP"
     gene            863..1171
                     /locus_tag="PKZ53_11605"
     CDS             863..1171
                     /locus_tag="PKZ53_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41127.1"
                     /translation="MTTQLKFILTLRHVRVLPKLFMLIERLGAECTDMQGHLVPGAGG
                     RLHLTLDCPQSRAHRLRPQIERLVDVTELYDAEQATLHEELLFQSDMVPVSALVEGSL
                     "
     gene            1168..2127
                     /locus_tag="PKZ53_11610"
     CDS             1168..2127
                     /locus_tag="PKZ53_11610"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNJ41128.1"
                     /translation="MSFQNATTVWKNGQLVPWEDATVHLSAHGLHYGTGVFEGIRCYD
                     TPEGPAVFRLREHIARMFASAWLHNIPIPFTQGELVQATLETVAANGLKSAYVRPIVF
                     FGSGSLGLLPNACPTEVAIMAWEWALLLGADSAEKGIRATISSWQKFDSNAMPATAKA
                     CGQYLNSVLATREARTRGYHEALLLNSAGNLAEGAGENLFLVKNKTLITNGQADDVLL
                     GITRDTVIQLARENGYQVTTRSLTKLDLLAADEAFVTGTAAEIVPLCEVDGYEIGDGH
                     RPVTRVLQQAFRDAVQGRDPNHTDWLSFVPSRESAQSEVEVRA"
     gene            2194..2622
                     /locus_tag="PKZ53_11615"
     CDS             2194..2622
                     /locus_tag="PKZ53_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ41129.1"
                     /translation="MVGHMNSFSESPESDCPVPPEFTSNDHALHIAWKNVPAIQFNDR
                     FTGKRVEGENVLIVCTEAQEGFIVPKHKHESEQISMVQSGSWHFNVAGNEFVAKAGDV
                     VHIPAGWEHSAVALETSTIIEIFTPPRRDWNDDEEYLWGV"
BASE COUNT          621 a          748 c          737 g          616 t
ORIGIN      
        1 aaccctatca gacctcctcc gattggcagg ccgaagcctt tgcccccggt gatgggttaa
       61 aactccccga atcgctcaac cgcagccaga tcccgcccac cgatgtcctc gataccctct
      121 ttcaacttct gcctgaaaac gcaatcgtca cgaccgatgt cggccagcat cagatgtgga
      181 ctgcccagcg ggcacgtccg attgatccgc gaaatttcat tacttccggt ggactcggca
      241 ccatggggtt tggcgttccg tcggctatcg gagcccagtt tgcctgcccg gaccggctgg
      301 tcgtggccgt ggtcggagac ggcggtttcc aaatgtcaat tgctgaactt gcgacgctca
      361 aacgccataa actgcctgtc aaagtgcttg tgatggacaa caaatacctt ggcatggtcc
      421 gtcagtggca ggaacttttt tatcaaggcc gctattcggc ggttgatttg cgcgacaacc
      481 ctgactttgc cgcaattgcg gtggcctacg ggctgacgtc cttcaaactc actgagcccg
      541 aagcggttga atctaccctt cgcgagtggc tggcaacgcc gggtccggcg ttgctgcact
      601 gtgaatgcca catcgctgaa aacgttttcc ccatgatccc cgctggcgcg gcgattgatg
      661 aaatgatgga agcaacccca actcgaccat aaacagggta tagggctgag ggctaagggc
      721 tgagggctaa gggctgaggg tgtaggactg agggctgagg gttgaaaaac aaatacccta
      781 atcccgatgt cttcagcccc aagcccttag ccccaagccc tcagccctac acccttagcc
      841 caaacataag gagaacccac ccatgacgac acaactgaaa ttcatcctga cgctgcggca
      901 cgtgagagtg ctgcccaagt tgtttatgtt gatcgaacgt ctcggcgcag aatgcaccga
      961 tatgcagggc cacctggtgc caggtgcggg tggtcggttg cacctgacgc tggattgtcc
     1021 ccaatcacgg gcccatcggc tgcgaccaca gatcgagcga ctggttgatg tgaccgaact
     1081 ctatgacgcc gaacaagcga cgctccacga agaactgctg tttcaatcag atatggtgcc
     1141 ggtgagtgca ctggttgagg gttcgttatg agttttcaga atgcaaccac tgtttggaaa
     1201 aatggccagc ttgtgccgtg ggaagacgcc acggttcatc tttccgccca cgggctgcac
     1261 tatggcaccg gcgtttttga aggcatccgt tgttatgaca cgcccgaagg accagcggtt
     1321 tttcggttgc gcgagcatat tgcccggatg tttgcctcgg cctggctgca caatattcca
     1381 attccattta cccagggcga gcttgtccag gcgacgcttg aaaccgtggc tgccaatggt
     1441 cttaagtcag cgtatgtgcg tccaatcgtc ttttttggaa gcggctcgct cggacttttg
     1501 cccaatgcct gcccgaccga agtggccatt atggcctggg aatgggcgct cctgctgggt
     1561 gcggacagtg ccgaaaaagg aatccgggcg acgatttctt cgtggcaaaa gtttgattcc
     1621 aacgccatgc cggcaacggc caaagcgtgc ggtcaatatc tcaattcagt cctcgccacc
     1681 cgtgaagccc gcactcgggg ctatcacgaa gccttacttt tgaattcagc cggaaatctg
     1741 gccgaaggcg cgggtgaaaa cctgttcctg gtcaaaaaca aaaccttgat taccaatggt
     1801 caggctgatg atgtcctgct cggcattacc cgcgacaccg tgattcaact ggcgcgggag
     1861 aacggctatc aggtcaccac ccgatcattg accaaacttg acttgctggc cgctgacgaa
     1921 gcctttgtca ctggtacggc agccgaaatt gtgccgctct gcgaagtaga cggctatgaa
     1981 attggtgacg gccaccgacc ggtgacgcga gtgctgcaac aggcatttcg tgacgcggtt
     2041 cagggtcgtg accccaacca cactgactgg ctttcgtttg tgccaagccg cgaatcagct
     2101 cagtcagaag ttgaggtcag ggcatagggt tgggtttgaa ttttccaacc acgaaccctc
     2161 atcttccacc ccttatatat aaaggagcat tttatggtag gccacatgaa tagtttttct
     2221 gaatcaccgg agtctgactg tccggtacca cctgaattca ccagcaacga tcatgcgctg
     2281 catattgcct ggaagaatgt tccagccatt caattcaatg accggtttac cggcaaacgc
     2341 gtcgaaggcg aaaacgtgct gatcgtctgc accgaagccc aggaaggctt catcgtgcca
     2401 aaacacaagc acgaaagtga acagatttcg atggtccaaa gcggatcctg gcacttcaat
     2461 gttgctggta atgaatttgt cgccaaagcc ggcgatgtcg tgcatatccc ggctggttgg
     2521 gaacacagtg ccgtggcact cgaaacttca accatcatcg aaatttttac tccgccgcgc
     2581 cgtgactgga acgacgacga agaatatttg tggggagtgt aattttaggg ctgagggctt
     2641 ggggctaagg gcttggggtt tagcgtttaa gccatagctt gcctgatgac taagattctc
     2701 accaaaacaa gttcaaaatg at
//
LOCUS       DAOQDO010000689         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31506_length_2715_cov_3.399248, whole genome shotgun sequence.
ACCESSION   DAOQDO010000689 DAOQDO010000000
VERSION     DAOQDO010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31506_length_2715_cov_3.399248"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKZ53_11620"
     CDS             complement(<1..341)
                     /locus_tag="PKZ53_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41130.1"
                     /translation="MKKYLALFSLVCGLFVLMSGTVYGQGTVVIAGGGAEGDQGDQTA
                     WSYQLYKKLVENGDKNGDGIIKVAILTSDPADQAAFIEGYFQWIGTTLGVTVQAKQYT
                     VQNRTDAGSSA"
     gene            complement(666..2483)
                     /locus_tag="PKZ53_11625"
     CDS             complement(666..2483)
                     /locus_tag="PKZ53_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ41131.1"
                     /translation="MANKKKPTKILYTILGVVLSLVFIPLVIVVWQLVEINREAVRSA
                     ERFSQIQVTEDVANQVETYVNGHRDQIAGIAQALEASNGIAELFKQTRAEQNLRLQRF
                     LTADPNLVLICIIPLTNLEGQGAFAFDPNRINNEEAGQVIANTVSKLTQKPNEPYLSE
                     PISFQSSNESAISMAEPVIAFREGEIPSVVIAVASMEPVFRYVNPPVRGKTNQKMLKD
                     GNRIVFVVNEKGMVVVHPDREMVYSKPDVNSWGIVQSWIQNKDLANQAEQFELNLNGE
                     KFSMLGSFNTVALSKDIKLGVISVINEESAYPSVPIMIRQAVKISVVIAVIAVGIGVL
                     LAFWVTLPVKKLAEGARAIATGEFSKRIHVNTQSEIGQLADAFNSMAEQIQRYINDLK
                     FAANENRQLFLGTVRALAEAIDGKDPYTRGHSERVMQYSVLIAEYMGMNPEEIEDIRI
                     AAILHDVGKIGIEDRILKKPAALTDEEYTIMKGHPQKGANIMSQIPQMKKYVPGMYYH
                     HECMDGKGYPLGLRGDQIPMMARIISVADTFDAMTTNRPYQRAMEPDFAVSRIYTFSG
                     TRYDPAVVKALDESLKSGRLSAIIQSYQQSQVQSSETAK"
BASE COUNT          671 a          652 c          670 g          722 t
ORIGIN      
        1 ttggctgacg agccggcatc agttcgattt tgaaccgtat actgtttggc ctgaaccgtg
       61 acacccagcg tagtgccgat ccactgaaaa tatccttcga taaatgccgc ctgatcagcc
      121 ggatccgaag tcagaatggc aactttgatg attccgtcgc catttttgtc gccattttca
      181 accagctttt tataaagctg atacgaccag gccgtttgat cgccttgatc gccttcagcg
      241 ccgccgccgg caatgaccac ggtgccttga ccatagactg tgccgctcat caagacaaac
      301 agcccacaca ccaaactaaa aagcgccaga tactttttca tgagaaccct cacgtacatt
      361 gaaactaagc acccggcatc gtgagccaga tgcagcgcag ttggtcgttg cctttgaaga
      421 agcagggatt gattctccaa cggcgaaata tttaattagt attgattgtt acagtgaaga
      481 gaaaaagagg taatagacgt tacaagcgta tccagatctg aataacttgt caacaatttg
      541 ttacaggaat gagacaaaat ttttttcggg gtgaaagctg tgggctacag aatcgtgaaa
      601 atgcaaaaag ggcacagcga actgtgccct tgagaggttg ttcaggatat caatgggtca
      661 ccagttcatt tcgccgtctc tgaactctga acctgtgact gttgatagct ctggatgatg
      721 gctgacaggc ggcccgattt gagtgattcg tccaatgctt tgaccactgc cgggtcatac
      781 cgggttccag aaaaagtgta gatccgcgat accgcaaaat caggttccat ggcccgctgg
      841 taggggcgat tggttgtcat ggcatcaaat gtatcggcca cgctgatgat gcgggccatc
      901 attggaattt gatcgcctct cagacccaac ggatagcctt ttccgtccat gcattcgtgg
      961 tgatagtaca tgcctgggac atattttttc atttgtggaa tttgcgacat gatattggca
     1021 cccttttgcg ggtggccttt cataatcgtg tattcctcat cggtcaatgc ggctggtttt
     1081 ttcaaaatgc ggtcttcaat gccaattttg cctacatcat gcaggatagc cgcgatgcga
     1141 atgtcttcga tttcctccgg gttcatgccc atgtattcag caatcaagac cgaatattgc
     1201 atcacgcgtt cagagtgccc tcgggtgtaa gggtcttttc cgtcaattgc ttcagccagg
     1261 gcgcggactg ttccgagaaa gagttgccgg ttttcatttg ccgcaaattt caagtcgttg
     1321 atatagcgct ggatttgctc agccattgag ttgaaggcat cggccagttg accaatttct
     1381 gattgggtat tgacgtggat gcgttttgaa aactcgccgg tggcaattgc ccgagcacct
     1441 tccgccagtt tcttgaccgg gagggtgacc cagaaagcga gtaagacccc aattccaacc
     1501 gcaatgacag caatcaccac gctaattttc actgcctgcc gaatcatgat cggtacggat
     1561 gggtaggctg attcttcatt gatgactgaa atgacaccca gtttgatatc tttactcagg
     1621 gcgacggtgt tgaaactccc cagcatcgag aacttttcac cgttcaggtt gagttcaaac
     1681 tgctcagcct gatttgccag gtctttgttt tgaatccacg attgcacaat gccccagcta
     1741 ttgacatcag gtttgctata gaccatctcg cggtccgggt gcaccaccac cattcctttt
     1801 tcattcacga caaacacaat ccgattgcca tccttgagca ttttctgatt agttttgcca
     1861 cgtaccggcg ggttcacata gcggaagact ggttccatgc tggccacggc aatcaccacc
     1921 gatggaattt caccctcgcg aaaggcaatc acgggttccg ccatcgaaat agctgattca
     1981 ttgcttgatt gaaaggaaat cggttcactc aggtagggtt cgttcggttt ttgagtcaat
     2041 ttggagaccg tgttggcaat gacctgtcca gcctcttcgt tattgatccg atttgggtca
     2101 aacgcaaagg caccctgacc ttccaggttt gtcagcggga taatgcaaat cagaaccaga
     2161 ttgggatcag ccgtcagaaa tcgctgcaga cggagatttt gctctgcacg tgtttgtttg
     2221 aagagttcgg caattccatt ggaggcttcc agggcctggg cgatgccagc aatctggtcg
     2281 cgatgcccat tgacataggt ctcgacttga ttggcgacgt cttcagtgac ctggatttgt
     2341 gagaaccgtt cggcactgcg tacggcttca cggttaatct cgaccagttg ccacaccaca
     2401 atcaccagcg gtataaacac cagcgataag acaacgccga gaatggtgta gagaattttg
     2461 gttggcttct ttttattcgc cacacgtcac ctcacatcct gacttgattt gcggggcagg
     2521 gaaagtgatc gagttcaggg caacacctgc ctgactccag attgctttgc gataacttct
     2581 gaaaattcga tttgatatgg tactgaaaga agtgggcagt gtagcacacc tgctcaaggc
     2641 tttgctctgg ggactttccg aacagaatga agaatgaaga atgaagaatg aagaatgaag
     2701 aatgaagaat gaaga
//
LOCUS       DAOQDO010000690         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31508_length_2715_cov_3.218797, whole genome shotgun sequence.
ACCESSION   DAOQDO010000690 DAOQDO010000000
VERSION     DAOQDO010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31508_length_2715_cov_3.218797"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PKZ53_11630"
     CDS             complement(<1..1453)
                     /locus_tag="PKZ53_11630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ41132.1"
                     /translation="METSDVQFQDMPQALTSTGIFTWKQTFVCLALLTCLAFGLRVAN
                     LGAAGFAEDEVHKVEAIAAYRQGDFAHNLEHPMLMKLAMGASLSVSEQWNQRVPVSCS
                     IPPEAAIRFPNCVFGALTTLVLGLLAAELFNPLIGILAGMFWATGMLSLAINRIGKED
                     TFLVFFLLLAYYAYLHAKHLGMSPLSPRHRQVQAHWYTLSGASFGLMIASKYFPHYWG
                     LLFLWEYLDHKLRPTNPLTNPIPRFVFPRFYGAMVGCFLIGNPIVWMSPIFSYVINYV
                     SEETVTHHGYMMMDMLFYNTPSHTAFTGGLPLYFYGLMLGIKTQPLVLMGLVVGVIVT
                     LRRIGQSQYFFIVFMTAFWLVPFSIIGAKWMRYTLSLLPFVYLVAAIGIYEIGNWGYR
                     LYRLLSSTRTCEHSFLEHHPDTQEHLVAAALAVITAIVLIPCLQQMPFYSLYTNTFGG
                     GEARAGTYFPHDEFYDAGVREALASIAQLAPPGA"
     gene            complement(1811..>2715)
                     /locus_tag="PKZ53_11635"
     CDS             complement(1811..>2715)
                     /locus_tag="PKZ53_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41133.1"
                     /translation="TVVAELKPEIKVEKPAPPVQSFEPLATSPPATPTSHTPLSSVTL
                     ASSASSLETNWQQMLQSARAAGDRGAEKRALTLMGSSLHSQGHTARARDNYQQALAIG
                     KETQSRQGEAALLNNIGETFRQEGRYADALAYYRLALRSAREFRDDRASEIALVNAGL
                     MYMRLGQIKEALSMLNQAQASNEVTQDQEMRAEALQALGEVYWIQKEYAKALDSCQEA
                     LKLAQDVGNSDLEWRAQWVIGRCRWLRQEKPLAQAALQASLAAIDRRFSELAGEDLRR
                     RLLSERQEISSLHEEWQRESGIRG"
BASE COUNT          708 a          710 c          687 g          610 t
ORIGIN      
        1 acgcaccggg cggtgccagt tgggcaatac tggccagggc ttcacgcact ccggcgtcat
       61 aaaattcatc gtgtgggaag taagttccag cgcgggcttc cccaccacca aatgtgttgg
      121 tatagaggct ataaaacggc atttgctgca aacacgggat taacacaatt gcagtgataa
      181 cagccagggc cgccgcaacc agatgttcct gggtatctgg gtgatgctcc aaaaacgaat
      241 gctcgcaggt tcgggtcgaa ctgagcaacc gatagagtcg gtatccccag tttccaattt
      301 catagattcc aatggctgct accaggtaga caaaggggag taacgacagc gtgtagcgca
      361 tccatttggc cccaatgatg gaaaatggca ccaaccagaa ggctgtcata aaaacaatga
      421 aaaagtactg gctttgccca atccggcgca gcgtcacaat caccccaacc accaggccca
      481 tcagcaccag cggctgggtt ttgattccca acatcaatcc ataaaaatag agcggcaacc
      541 ccccggtaaa ggcagtgtgt gatggcgtgt tgtaaaacaa catatccatc atcatatacc
      601 cgtgatgggt caccgtttcc tcactgacgt aattgatgac ataggaaaag atgggtgaca
      661 tccacacaat agggtttcca atcagaaaac aacccaccat ggccccgtaa aatcggggaa
      721 agacaaaccg gggaatggga ttggtcagcg gattggttgg acggagctta tgatcgaggt
      781 attcccacaa aaacaacaat ccccaatagt ggggaaagta tttagaagca atcattaagc
      841 caaaggacgc gcccgaaagg gtgtaccagt gagcctgaac ctggcggtgg cggggagaca
      901 gtggactcat gcccaggtgt ttggcatgga ggtaggcata gtaggcgagc aacaaaaaga
      961 agaccagaaa cgtgtcttct tttccaatcc gattgattgc cagtgatagc attcccgtgg
     1021 cccagaacat cccggccaaa ataccaatca gcggattgaa caactccgcc gccaggagcc
     1081 caagcacaag cgttgttaag gcaccaaaca cacagttggg aaaccgaatg gcggcttccg
     1141 gaggaattga acagctcact ggaacacgtt gattccattg ctccgacaca ctgagactgg
     1201 cgcccattgc cagtttcatc agcatcggat gttccaggtt gtgggcaaaa tccccctggc
     1261 gataggcagc aatcgcttca actttgtgga cttcgtcttc agcaaaacca gcagcaccta
     1321 aattggccac acggagccca aacgccaaac aggtcaggag tgccagacac acgaaggttt
     1381 gcttccaggt aaaaatgccg gttgatgtca gggcctgcgg catgtcctga aactgaacat
     1441 ctgatgtctc caaagatctc acgtagtatt ggtcctcaaa cagtatccgg ttggacagaa
     1501 tcagaaacgt ataccagtca gtagtcagta atcagtagtc agtagttcac taagtttatt
     1561 taactgaata acttgactgt cttctcatac aaccacttca ttccaaaatg gtatgacaag
     1621 ttatgaagcg atgaacggag aagaatcaaa aaccagggta tttgcttttc aacaagatag
     1681 gttttcttca ttcttcattc ttcattcttc attcgtttcc actgaaagtg tgcttttcag
     1741 gggtctacaa agagaatgat tgggtgtgtt ccccaacgaa ccagtcagtc ccttccctca
     1801 agcattttcc ttatccacgg ataccagatt cacgctgcca ttcttcgtgc agcgagctaa
     1861 tttcctgacg ttctgagagt aaacgccgcc gcaagtcctc acctgcgagt tcactgaagc
     1921 ggcggtcaat cgcagccaga ctggcctgta gcgcagcctg agcaagtggc ttttcttgcc
     1981 ggagccagcg gcaccgaccg atcacccact gagcacgcca ttcgagatca gaatttccaa
     2041 cgtcttgagc cagttttaat gcttcctggc acgaatcaag tgccttggcg tattcctttt
     2101 gaatccaata gacttctccc aatgcctgca aggcttcagc ccgcatttcc tggtcctggg
     2161 tgacctcatt gctggcctga gcctggttga gcatcgaaag cgcctctttg atctgaccca
     2221 gccgcatata catcagacca gcattgacca gggcaatttc ggatgcccga tcatcacgaa
     2281 attctcgggc gctccgcagt gccagccggt aataggccag cgcatccgca taccgtccct
     2341 cctgtcgaaa ggtctcgccg atgttgttga gcaacgccgc ttcaccctga cgcgattggg
     2401 tttctttgcc gatggctaaa gcttgctgat aattatcgcg ggcacgggcc gtgtgaccct
     2461 ggctgtggag cgaagaaccc atcagtgtga gggcacgctt ttcggccccg cgatctccgg
     2521 cagcccgggc tgattggagc atctgctgcc agttggtttc aagcgaactt gcggaactgg
     2581 caagagtaac ggaagagagt ggggtatggg aagtcggtgt ggcaggtggc gatgtcgcca
     2641 gtggttcaaa gctctgcact ggcggggcag gtttctctac cttgatttca ggctttagtt
     2701 cagccacaac agttg
//
LOCUS       DAOQDO010000691         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31517_length_2715_cov_2.484962, whole genome shotgun sequence.
ACCESSION   DAOQDO010000691 DAOQDO010000000
VERSION     DAOQDO010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31517_length_2715_cov_2.484962"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="PKZ53_11640"
     CDS             complement(<1..1596)
                     /locus_tag="PKZ53_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNJ41134.1"
                     /translation="MKQAALFNFAPIPSAIEVREAVPLPTYRYVNRAAEFVPFVSELC
                     AAPVLAIDTETTGLDPLTDRIRLLQIAAPGLPVFVLDLFHLTDLAPVQEVLASNHVKV
                     FQNAKFDLKFLSRAGLEVGGQLFDTMLAGQIISCGLSNQRHSLEALVANYLKQSLSKT
                     EQVSDWSARELSSEQLRYAALDAAVLLPLREALISKLKAADVIKCAKIEFDCVRAVAE
                     MELAGISLNLDRWEALRKQISTVRDTLAIRLREAFGGGFLGEINLDSPTQVQRALAMR
                     GIQVSGTSRWELARFSDHPEVRLFQEYRKASKLCSAFLDTLPKFIHPQTGRLHPVYEQ
                     CLVVSGRFSCHSPNLQQLPHDREFRACFIPAEGHSFVIADYSQIELRVAAQLSQDERM
                     MDAYRANQDLHLLTASLITGKPMGSVTKADRQAAKAVNFGLLYGQGAQGLKSYAKQSY
                     GVDLTLADAERFRDRFFLAYQGIAAWHRQTARNQKKIRETRTLIGRRRLIEKPLELPT
                     LLNLPVQGTAADITKLALAKILPQ"
     gene            complement(1837..2325)
                     /locus_tag="PKZ53_11645"
     CDS             complement(1837..2325)
                     /locus_tag="PKZ53_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41135.1"
                     /translation="MLEIRRAVETDFDQIWEIFHPVVKRGDTYMYAPDTTKDQARQIW
                     MSKDMATYVACDGAEIVGTYILRPNQPALGAHVVNAGYMVKPNTQGKGIGRAMCGHSL
                     REARQAGFLAMQFNAVVSTNENAVALWKKMGFSIVGTVPKAFNHQELGLVDIFIMHQF
                     LD"
BASE COUNT          674 a          717 c          676 g          648 t
ORIGIN      
        1 ctgcggcagt attttggcca gcgccagctt ggtgatgtcg gctgccgttc cttgaaccgg
       61 caggttgagc aatgtcggga gttccagcgg tttttcaatt agccgtctcc gaccaatcaa
      121 cgtccgtgtt tcacgaattt ttttctggtt gcgtgctgtc tggcggtgcc aggcagcaat
      181 gccctgatag gcgagaaaga accggtcgcg gaagcgttcg gcatcggcca gcgtcaaatc
      241 tacgccatag ctttgtttgg cgtaactttt caacccttgc gcaccctgcc catagagcaa
      301 cccgaaattc accgccttgg ccgcctgccg gtcggctttg gttactgatc ccatcggttt
      361 gccagtgatt aaactcgcgg tcaacaaatg caaatcctgg tttgcgcgat aggcatccat
      421 cattcgctcg tcctgactca actgcgccgc aactcgaagt tcaatctgcg aataatcggc
      481 aattacaaac gaatgacctt ccgccggaat aaaacaggcc cgaaattcac ggtcgtgcgg
      541 caattgttgt aagtttggcg aatgacagct aaaccgtccc gacaccacca ggcactgttc
      601 atacaccggg tgcagccttc cggtctgcgg atggataaac ttcggcagcg tgtcgagaaa
      661 ggcgctgcag agctttgacg ctttgcggta ctcctgaaac aaccgcactt ccggatggtc
      721 actaaaccgg gccagctccc aacgactggt acccgaaacc tggatgccgc gcatcgccag
      781 cgctcgttga acctgggttg ggctgtcaag attgatttcg cctaaaaacc cgccgccgaa
      841 ggcttcgcgc aatcgaatgg caagcgtgtc acgaaccgtc gagatttgtt tgcggagcgc
      901 ttcccaccga tccagattca ggctaattcc ggccagttcc atttcggcaa cggctcgcac
      961 acagtcaaat tcgattttgg cgcatttgat gacgtcagcg gctttgagct ttgaaatcaa
     1021 cgcttcccgg agtggcaaca ggactgccgc atcgagtgcc gcgtagcgaa gttgctctga
     1081 tgaaagttcg cgggctgacc agtcgctgac ttgctcggtt ttggaaagcg attgttttaa
     1141 ataatttgcc accagggctt cgagcgaatg acgttgattg gacaaaccac aactgatgat
     1201 ttgcccggcc agcatcgtgt caaaaagctg cccgccgacc tccagtccag cgcgtgacaa
     1261 aaatttcagg tcaaatttgg cattctgaaa cactttgaca tggtttgatg ccagcacctc
     1321 ttgaaccggc gccaggtctg tcagatgaaa caaatccagc acgaacaccg gcaagcccgg
     1381 cgccgcaatt tggagcaggc gaatccgatc tgtcagcgga tcaagtcccg tggtttcggt
     1441 atcaatcgcc agtactggcg cggcacacaa ttcagaaaca aatggaacaa actctgctgc
     1501 ccgattcaca tagcggtatg tagggagtgg aacagcttcc cggacctcga tggctgaggg
     1561 gataggcgcg aagttgaaca aggcggcttg tttcatagca aggcttcaat tccagggctc
     1621 agggcttggg gctgaagata caagggttgg gttgagttga acctgtctca tcaacaaaaa
     1681 caacgtccat ttttattatt aagttatatc actaaacgat gactcagcaa aacaaaactc
     1741 accacagagg acacagagga acacagaggc aacctgagac tcctctgtgt tcctctgtgt
     1801 cctctgtggt gaaaaaagaa aggttttcac ccgctctcaa tccaaaaact gatgcatgat
     1861 gaaaatatct accaatccta actcttgatg attaaatgct ttgggtacag ttccaacaat
     1921 tgagaacccc atttttttcc acaacgcgac tgcattttca ttggtgctca caaccgcatt
     1981 gaactgcatc gccagaaaac cagcctggcg ggcttcccgg agcgaatgtc cacacattgc
     2041 ccggccaatt cccttgcctt gggtgttggg cttgaccata tagcccgcat tgaccacgtg
     2101 ggcgccaagc gccggctggt tgggcctcaa aatgtaggtt ccgacgattt ccgctccgtc
     2161 acaggcaaca taggtcgcca tatcttttga catccagatt tggcgggcct ggtcttttgt
     2221 cgtgtcgggg gcatacatat aggtatcgcc acgtttaacg actggatgga aaatttccca
     2281 aatttgatca aaatcagttt caacggctcg gcgaatttca agcatagtat ttagggttca
     2341 gggttcaggg ttcagggaaa tgcaattttt ccagttattg agcgatagat cagaaaaagg
     2401 ttcagcgtat cattctgaga caagaacgtt tctgcttcta ccggattttg tgttggaggc
     2461 gaatcagaag aaccgataag tcagtaccac ccgcgtcagt gggtgggtcg gtcagctttc
     2521 caatcccacc cgctcacgcg ggtggtactg acaagtcctt tatttccaac actgacttga
     2581 cgaaacacaa aatccggtag aagcagaacg attgatctga aaggctatac aatgttcaga
     2641 acgacaccca acgacacctc ccattttaaa gaggagaact ttcatgctgc tatttccgac
     2701 tgaacgcata ccctc
//
LOCUS       DAOQDO010000692         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31570_length_2712_cov_2.865638, whole genome shotgun sequence.
ACCESSION   DAOQDO010000692 DAOQDO010000000
VERSION     DAOQDO010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31570_length_2712_cov_2.865638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKZ53_11650"
     CDS             <1..911
                     /locus_tag="PKZ53_11650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ41136.1"
                     /translation="LVEASSENISISTPVSGLVTRVAVKVGDFVKAGAPLFSLDDRDL
                     QAELMVRKAALDVARQKLKRLEESPRPEEIPPAEARVREAESALGDAKIQLQLIESVT
                     DKRAIRDEDLQRRRFAVQAAQARLEQEQTKLALLKSGSWKPDLEVARSEVQQAEAQVK
                     RIQTDISRLTVQALTDGRILQCNVRPGEYAQSGQLPKPLMVVGDVGTLHIRTDVDEND
                     AAKVKAGAQAYAYLRGKTDRQIPLEFVRFEPFVIPKKSLTGDSTERVDTRVLQVVYRV
                     VDQSAPIFVGQQMDVFIDGSTGQKTE"
     gene            1004..2425
                     /locus_tag="PKZ53_11655"
     CDS             1004..2425
                     /locus_tag="PKZ53_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ41137.1"
                     /translation="MNRKLATLLAIAILGGGCAIPPKRVRIEVEPPAQWSAASPQTPP
                     ETAELKTWWKTFQDPTLDSLVERALTSNFDLKLAAARIREARGARGVTAAARYPAINQ
                     TDSFQRIRGGFAQGVTRVGSSGGGTLVTPFETNNFQIGFDATWEIDVFGGVRNSVNAA
                     QADVNVAIESQRDVLVSLLGELARTYVELRGLQRRVAVVQKNIAVQQETVHLTKVRSQ
                     AGLATELDVSRAEAQLATTEAVLPQLEATMAQAIHRLGVLTGQTPGALIAELTPVEPV
                     PSRPPSVPVGLPADLLRRRPDIRRAEAEIVAATARLQVAQAERFPKFSLTGSFGRQAT
                     SVSGFTLGAGNFFAIGPGIRLPIFTGGRIKSNIEIQDARLEQCALQYQQTLLIALEET
                     ENALVAYAHEEARRQKLVAAVQSSQQAAEFANELYVRGLSDFLSVLAAQQTQYTAEDE
                     LAQSETSTVESAVRLYKALGGGW"
BASE COUNT          626 a          677 c          762 g          647 t
ORIGIN      
        1 ggctggttga agccagttca gaaaatatct cgatcagcac gccggtttca gggctggtca
       61 cacgagtcgc ggtgaaagtt ggagattttg tcaaagccgg ggcgccgttg ttttcccttg
      121 atgatcggga tttacaggcc gaattgatgg ttcggaaagc ggcgcttgat gtcgcccggc
      181 aaaagttgaa gcggttggaa gaatctccgc gcccggaaga aatcccaccg gctgaggccc
      241 gcgtccgcga agcggaatct gcgctcggag atgccaaaat ccagcttcag ttgattgaaa
      301 gcgtgaccga caaacgggcg attcgcgacg aagatttaca gcgacggcga tttgcggttc
      361 aggcagccca ggctcgattg gaacaggaac aaaccaaact ggcgttgctt aaatccggat
      421 cctggaagcc agatttggaa gtggcccgtt ctgaagtcca gcaagctgag gctcaggtca
      481 aacgaatcca gactgatatc agccggttaa cggttcaggc cctgaccgat gggcgcattc
      541 tgcaatgtaa tgttcgtcct ggcgaatacg cccagagtgg tcagctcccg aaaccgttga
      601 tggtcgtggg cgatgtcgga acactgcaca tccgcaccga tgtggatgaa aatgatgcgg
      661 cgaaagtaaa ggctggggcc caggcgtatg cttacctgcg tggcaaaacc gaccgtcaaa
      721 ttccacttga atttgttcga ttcgagccgt ttgtgattcc aaagaaatca ctcacaggcg
      781 atagcaccga acgggttgat acacgggtgt tgcaggtcgt ttatcgagtg gttgaccagt
      841 ctgctccgat ctttgtcggt cagcaaatgg atgtgtttat tgatggaagc accgggcaaa
      901 aaacagaata gggttccggg ttccgggttc cgggttcttc aaaatgggtt tcttttggtc
      961 ccttttgttt tttccaagcc cagaaccctc aaagaggcat tttatgaatc gtaaactggc
     1021 aacgcttctg gcaattgcaa ttttaggtgg cggctgtgcg attccaccca aacgggtcag
     1081 aatcgaagtt gaacctcccg ctcagtggag tgcggcttca cctcaaacgc cccctgagac
     1141 cgccgagttg aagacctggt ggaagacttt tcaggatcca accctggact cgctggttga
     1201 acgagcgctc acttcaaatt ttgacctcaa actggcagcg gcacggattc gcgaagcccg
     1261 tggtgcccgt ggtgtcaccg ccgccgcccg gtatccagcc atcaatcaaa ctgattcgtt
     1321 ccagcggatt cgcggaggct ttgcccaggg agtgacacga gttggttcat ctggtggcgg
     1381 aacactggtg acgccgtttg aaaccaacaa ttttcagatt gggtttgatg caacctggga
     1441 aattgatgtc tttgggggtg ttcggaattc agtcaacgcg gcccaggcgg acgtcaacgt
     1501 ggcgattgaa agccagcgcg atgtgctcgt gtcgctttta ggcgaactgg cccgaaccta
     1561 tgttgagttg cgtggtcttc agcgtcgggt ggctgtggtt cagaaaaaca ttgccgttca
     1621 acaggaaact gttcatttga cgaaagttcg ttcacaggct ggtctggcga cggaactcga
     1681 tgtttcgcga gctgaagctc aactggcgac caccgaagcg gtcctgccac aattggaggc
     1741 taccatggca caggccattc accggttggg ggtactgact ggtcaaactc caggggcact
     1801 catcgccgaa ctcaccccag ttgagcctgt gccgtcgaga ccaccttcag tgccagttgg
     1861 attgccagcc gatttgctac ggcggcgccc agatattcgg cgggcagaag ctgaaattgt
     1921 ggcagccacc gccagacttc aggtggccca ggccgaacgc tttccaaaat tttcattgac
     1981 tggctccttt ggccgtcaag ccacgtcggt cagtgggttt acgcttggcg ccgggaattt
     2041 ctttgcgatt ggtcctggca tccgattgcc gatttttacc ggaggacgaa tcaaatccaa
     2101 tatcgaaatt caagacgccc gtctcgaaca atgtgccctt cagtatcagc aaaccctgtt
     2161 gatcgcgctg gaggaaactg aaaacgccct ggtggcctat gctcacgaag aagcccggcg
     2221 acagaaactg gtggcggcgg ttcaatcaag tcagcaagct gccgaatttg ccaacgagtt
     2281 gtatgtgcgt ggtctctctg attttctttc ggtgctggcg gctcaacaaa cccagtatac
     2341 ggcggaagat gaacttgccc agagtgaaac aagcacggtt gaatccgctg tccgactcta
     2401 taaagcgctt gggggcggat ggtgatggac aaaagctcgg gctgaaggac atcgggcttg
     2461 gggcttgaag ctgaagaagt tgggctgaag agctggtttt attcgtcatt ccagcagcta
     2521 ccctgagccc atcatctcct gtttttattt cgatatttgt gaacgcgttc acaacggtct
     2581 aaaatattgt aaataaatat tttaaatggt cctagccagt tcaggtcata tagtttcccg
     2641 ctaaattcaa gatttaatta ttctgctttc acgccgaacg cctctggttg gcaatttaca
     2701 tttcacgtga ca
//
LOCUS       DAOQDO010000693         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31598_length_2711_cov_2.606928, whole genome shotgun sequence.
ACCESSION   DAOQDO010000693 DAOQDO010000000
VERSION     DAOQDO010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31598_length_2711_cov_2.606928"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKZ53_11660"
     CDS             complement(<1..695)
                     /locus_tag="PKZ53_11660"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ41138.1"
                     /translation="MAKLIVYGIQPQPVVIPLRGRFTIGRDRNCTLRLEDACISRLQA
                     VIETSGGEAVICDGGSSNGTWLNGQLIQFEEKLSDGDEVIFGGGIRATFTHHSTLPHL
                     APSRTSLARSKPEVTSSLGPKPSQAISQSVASDLPQPVAKIWLAGFGALLCLINFPII
                     WFITQRPQNSAPLSNNQSSVSPQSPRPPARSNPINEVQENSKPTPTAPVSASYPDVQQ
                     QLESVSAVVTHSHL"
     gene            complement(750..1406)
                     /locus_tag="PKZ53_11665"
     CDS             complement(750..1406)
                     /locus_tag="PKZ53_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ41139.1"
                     /translation="MSRRIFIYLCLIITVLVGTLGVLAAPQQNRGLQPFNLQFKEPVT
                     VLLKQGGTFIPVSRDYPFRQGDQIKFKIQSKLNGFVSILNITPTGQARLLSDQSQLSI
                     PVFSDQSCEIPAGQQAFQFDEERGVELIRFYLSPAPLPNLNTWIENLNPQQTDSQPVI
                     AYSPASRSVTFQPKKADKTPPPSSSTLPSNTSPAQLRTVNRTQPQVEVIELQLKHVGR
                     "
     gene            1703..>2711
                     /gene="mgtE"
                     /locus_tag="PKZ53_11670"
     CDS             1703..>2711
                     /gene="mgtE"
                     /locus_tag="PKZ53_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNJ41140.1"
                     /translation="MSAKTKNTMTPERLAKLRDELRSNPQAVSAVIDRLKAPDVAEVL
                     NDFLLDDAILAIMTLDPDEIVAVFNEPSFERRPALIQLLHIEQAARIITGLSSDERVD
                     IIQKLVPEFRARLMPYLPDKIQRETNLLLEFDPATAGGIMSTEYVRLDPSQTVAQALE
                     FVRSISKNRLHVYSAYVLDPQDRLLGAISLRDLVVSDPEQLVSQIMRKYPISVHFKED
                     QELVARKLAKYNLLAVPVVDDSQRILGFVTVDDALDVMVEEQTEDLQKLGGMQALEDG
                     YMNTPLWQLAQKRAGWLVVLLLGEMLTATAMSYFEGEIQKAVVLALFVPLIISSGGNS
                     GS"
BASE COUNT          675 a          619 c          708 g          709 t
ORIGIN      
        1 aggtgagagt gcgtaaccac ggctgatacc gattcgagtt gctgctgtac gtcaggataa
       61 ctggctgata ctggtgcggt tggtgttggt ttggagttct cctggacctc gttgattggg
      121 tttgaacggg caggtggacg tggtgattgg ggtgaaacag atgattgatt attgctcaac
      181 ggcgcagaat tttgtggccg ttgagtgata aaccagatga tggggaagtt gataagacac
      241 agcagggctc caaagccagc cagccagatt ttcgcaacgg gttgtggtaa atctgaggca
      301 actgactgac ttatggcctg gcttggtttc ggtcccagtg acgatgtgac ctcaggtttc
      361 gaccgtgcca gactggttcg agacggagca agatgcggta gtgttgaatg atgagtgaat
      421 gtggcacgga ttccgccgcc aaaaataacc tcatctccgt ctgataattt ttcttcgaat
      481 tgaatgagct gcccatttaa ccacgttcca ttactgcttc caccatcgca aatcactgct
      541 tcaccaccac tggtctcaat gacggcctgg agtcgtgaaa tacaggcgtc ttcaagccgc
      601 aaggtgcaat tgcggtctct gccgatggtg aatctccctc gaagagggat caccaccggc
      661 tgtggttgaa ttccatacac aatgagtttc gccattttag ataaaatcag acgcttgaaa
      721 gggcctgttt cagccgtgag caggaatcct tatcggccaa catgcttgag ctgaagttca
      781 atgacttcaa cctgaggctg ggtccggttg accgtgcgaa gttgagctgg actggtattt
      841 gaaggaaggg tactgcttga cggaggcggt gttttatccg ctttttttgg ttgaaacgtt
      901 acactccgcg aggccggact ataggctatc actggctggg aatctgtctg ctgaggattg
      961 agattctcaa tccaggtatt gaggtttgga agtggcgccg ggctcaaata aaaccggatc
     1021 aattcgaccc cacgttcctc atcaaattgg aacgcttgct gaccagccgg tatttcacaa
     1081 ctttgatcag aaaatactgg aatcgaaagc tgggactgat cagataacag ccgggcctgg
     1141 ccagtcggag tgatattcaa aattgaaaca aaaccattca attttgattg aattttgaat
     1201 ttaatttggt ctccctgtcg aaatggatag tcgcgagaga cagggataaa cgtcccgcct
     1261 tgtttgagta aaacagtgac cggttcttta aattgaaggt tgaatggctg aagacctcgg
     1321 ttttgttgtg gggcggccag tacccccagc gtcccaacca aaacggtgat gatcaggcac
     1381 aggtaaataa atattcggcg cgacataaca gatcaatcct cctgagaatg caaatgggcg
     1441 taagcttttt gtcaggaagg gcaatctttg tgccagagat acgttaaatt gagttaataa
     1501 attgagtctg ataagattga ttatgtcatt gactgcatta cagcaccttc ctgtagacag
     1561 cactctgaat caggagtaaa agcaaccaca catcccgttc tatccaacaa cctgtcccgt
     1621 gtttattcaa tccacactgg ttcagccgtc tttctcttct tgaacccaaa tcctgtcttt
     1681 tttgagtgag gagcccagat ctgtgtccgc aaaaaccaag aacacaatga ctccggaacg
     1741 actggccaaa ctccgggatg aactacgatc aaacccgcag gccgtaagcg ctgtgattga
     1801 ccgtctcaag gcgccggatg tggctgaagt cttaaatgat ttcttgctcg atgatgcgat
     1861 actggccatc atgacgcttg atccagacga aattgtggcg gtttttaatg aacccagttt
     1921 cgagcgacgt ccagctttga tccagttgct gcatatcgaa caggcagcca ggatcatcac
     1981 cggtctttct tctgatgaac gggtggatat tattcaaaag ctggtgcctg agttccgggc
     2041 gcggttaatg ccctatctcc cggacaaaat tcagcgggaa accaatcttt tgcttgagtt
     2101 tgacccggca accgctggtg gaattatgtc cactgaatat gttcggctgg acccatccca
     2161 aaccgtagct caggcccttg agtttgttcg gtccattagt aaaaaccgac ttcatgtcta
     2221 ctcggcctat gttttggatc ctcaggaccg cttattaggc gccatttcac tgcgtgatct
     2281 ggttgtgtct gacccggaac aactggtttc ccaaatcatg cgcaagtacc caatcagcgt
     2341 tcatttcaaa gaagatcagg aactggtggc ccgaaaactg gccaaataca atctgctggc
     2401 ggtcccggtt gtggatgatt cgcaacgaat cctgggattt gtgaccgttg atgatgctct
     2461 ggacgtgatg gtcgaagaac aaaccgaaga cttacaaaaa ctgggtggga tgcaggcgct
     2521 cgaagatggg tatatgaata cgccactctg gcaacttgcc cagaaacgcg ctggctggct
     2581 ggtggtgttg ttacttggtg aaatgctgac agcaactgcc atgtcatact tcgaaggcga
     2641 aatccagaag gcggtggtgc tggcgttgtt tgttcccttg atcatttcca gcggaggcaa
     2701 ttcaggatct c
//
LOCUS       DAOQDO010000694         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31723_length_2705_cov_3.187170, whole genome shotgun sequence.
ACCESSION   DAOQDO010000694 DAOQDO010000000
VERSION     DAOQDO010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31723_length_2705_cov_3.187170"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKZ53_11675"
     CDS             complement(<1..545)
                     /locus_tag="PKZ53_11675"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNJ41141.1"
                     /translation="MKNVVVIGMQWGDEGKGKIVDLLAPHFDIVTRYQGGHNAGHTLI
                     INGRKHVLHLVPSGIMHDGKMCVIGNGLVIDPAELMKEITELESHGIEVRDRLLISNR
                     AHLILPYHRALERLSEETRGAQKVGTTLRGIGPSYEDKYGRRGIRAGDVLYPEIFKDK
                     LESNVAEANQVLAARSASKAEP"
     gene            544..879
                     /locus_tag="PKZ53_11680"
     CDS             544..879
                     /locus_tag="PKZ53_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41142.1"
                     /translation="MTEIVLSQLSSVSENTRTHRSIAHNAPQRLNVRYDDHQRMVGEE
                     GVCGTLWYGGFSKVHSRMSQNEISTQVLFLTSPTQSYFQTPALIPLVQTSYNTHTKNR
                     IRYFIFYKM"
     gene            892..2547
                     /locus_tag="PKZ53_11685"
     CDS             892..2547
                     /locus_tag="PKZ53_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ41143.1"
                     /translation="MQSSQIKETSSQNHQVIVVGLDSCDPVLVQRWAREGKLPFLSSL
                     MKTGVWSPLVSAHGLFPDMPWVSCTTGVVPAKHGFFNFMQLKRGTTEFVRRSPSSIRH
                     YPFWWLLRGVGKRVAIFDVPKTYPLANIDGVQVAAWGEHYPLIEQSSLPKELIEDLVA
                     KFGTYRHAKEVVSPRTVKTERHIYDLLRSGIEQRTQAIAYLLKQENWDLFFTVFSESH
                     YAGHQFYHHHDTSHWAHDAEWAAKLDDPLPASYTRLDRALAELFQQRSADVTLILVSV
                     HGIATNYSGNHFMPDILEKLGYQVRAEHPAAPTSTTGQLLNLTASIRNLIPNVAREWI
                     NEHLVPEAVHDRAYFGQFSSRIDWRQSKAFFMPSDHFEGFISINLHGREPFGTVQPGA
                     EYDQVCTALISDLKQLTNPDTGRSAVRDVIQVSKLMTGPFLFNLPDLVVQWADDAYIT
                     SVHHPRFGILSDEHFDLRKSQHTTDGFLIATGKHINHQASLINATTLDIAPTLLYLMG
                     QPIPNDYDGRVLTELLDPEFVNRTPIQSCDRPLIIPEEMVLSG"
BASE COUNT          679 a          706 c          639 g          681 t
ORIGIN      
        1 ggttccgctt tagaggcact tcgggcggcc agcacctgat tggcttcggc aacgttggat
       61 tcgagcttgt ctttgaaaat ttccgggtag agcacatctc cggcccggat tccccggcgg
      121 ccatatttat cctcgtaact tggcccaatt ccacggagtg tggtccccac tttttgggca
      181 ccacgggttt cttcacttaa ccgttcgagg gctcgatgat acggcaaaat caaatgggca
      241 cgattgctaa tcagcaaccg gtcgcggacc tcaatcccat gggattcaag ttcagtgatt
      301 tctttcatca attcagccgg atcaatcacc agaccattgc caatcacaca cattttgccg
      361 tcgtgcataa tccctgacgg caccaaatgg agcacatgtt tgcgtccatt gataatgagg
      421 gtgtggccgg cgttgtggcc gccctgatac cgggtaacga tgtcaaagtg tggcgccagc
      481 aaatcaacaa tcttcccttt gccttcatcg ccccactgca ttccaataac aacaacattt
      541 ttcatgacgg aaattgttct ttctcaactc tcatcagtgt ctgaaaacac acgaacccac
      601 agaagcatcg cacacaacgc cccccaacgc ctgaatgtgc gttatgatga ccatcagaga
      661 atggttgggg aagaaggtgt gtgtggtact ttgtggtatg gtggtttttc caaagtacat
      721 tcaagaatgt cacaaaatga gatttcaacg caagttctct ttctcaccag cccaactcaa
      781 agctattttc aaacgccagc cttgattcct ttggttcaga ccagttacaa cacccacaca
      841 aagaaccgca tacgatactt cattttttac aaaatgtgag cgattctggt catgcaatct
      901 tctcaaatca aagaaacctc cagccaaaac catcaagtca ttgtggtagg tcttgattcc
      961 tgtgatccgg tgcttgtaca gcgttgggct cgtgaaggaa aattgccgtt tttaagttcc
     1021 ttgatgaaaa ccggggtctg gtcgccgctg gtttcagccc acggattatt tccggatatg
     1081 ccctgggttt cctgcacaac cggcgtggtg ccagccaaac atggtttttt caactttatg
     1141 caactcaagc gggggacaac tgagtttgtc cggcggagcc cctcttcgat tcggcactat
     1201 ccattctggt ggctgctgcg tggcgtgggg aaacgggtgg caatttttga tgtccccaaa
     1261 acctatccgc tggccaatat tgatggggtt caggtggccg cctggggtga acattacccg
     1321 ttgattgagc aaagttcgct tccgaaagaa ttgattgagg acctggtggc gaaatttggc
     1381 acctaccgac acgccaaaga agttgtttcc ccccgaactg tcaaaactga gcgtcacatc
     1441 tacgatctgc ttcggagcgg aatcgaacag cgaacacaag ccatagctta tctgctcaaa
     1501 caggaaaatt gggatttatt ctttaccgtt tttagtgaat cccactatgc cggacaccag
     1561 ttttaccatc accacgacac ctcacattgg gcacatgatg ccgaatgggc ggccaagcta
     1621 gatgatccgt taccagcaag ctatacccgc ctggatcggg cactggccga gttgtttcaa
     1681 cagcggtcgg ctgatgtcac actgattctg gtttcagtcc atggcattgc aaccaattac
     1741 tctggcaatc actttatgcc agacattctg gaaaaactcg gctatcaggt ccgggctgag
     1801 catccagccg caccgacctc aaccacgggt caacttctga acttgacggc gtccattcga
     1861 aatctgattc ccaatgttgc gcgcgaatgg atcaatgaac atctggtacc ggaggcagtt
     1921 cacgaccggg cctattttgg acaattcagt agtcggattg actggcgcca aagcaaggcg
     1981 ttttttatgc ccagcgacca ctttgaagga tttatcagta tcaatctgca cggtcgtgaa
     2041 ccttttggga cggttcaacc tggtgctgaa tatgatcagg tctgcacagc cttgatctca
     2101 gatctcaagc aattgaccaa ccctgatacc gggcggagtg cggttcgcga cgtcattcag
     2161 gtctcaaaac tgatgaccgg cccattttta ttcaacctgc cggatctggt tgtccagtgg
     2221 gcggacgatg cctatatcac atcagtgcat caccccaggt ttggaatcct ttccgatgaa
     2281 cactttgacc tgcgaaaaag ccagcacacc actgatggat ttctgattgc gaccggaaaa
     2341 cacatcaacc atcaggccag tctcatcaat gccaccacac tcgatattgc cccaacattg
     2401 ctgtatctga tgggacaacc aattccgaat gattatgatg gacgggtact gactgaactc
     2461 cttgacccgg aatttgtgaa tcgaacccca atccagtcct gcgaccgacc actgatcatc
     2521 ccggaggaaa tggtgttatc gggctgaaaa aaccagggct gaagaaaacg ggctgggagc
     2581 taaagaaacc agggctgggg gctgaagaaa acgggttgaa gaaatcagcc atgaattaat
     2641 ttcctgtcac gctgtcacct tgtcattgtg tcatcatcac ggtttggaat aaggctatga
     2701 agatt
//
LOCUS       DAOQDO010000695         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31751_length_2704_cov_3.091733, whole genome shotgun sequence.
ACCESSION   DAOQDO010000695 DAOQDO010000000
VERSION     DAOQDO010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31751_length_2704_cov_3.091733"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..696
                     /locus_tag="PKZ53_11690"
     CDS             448..696
                     /locus_tag="PKZ53_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41144.1"
                     /translation="MHKIIRSIRWYDKQGEDFKGELELREIPVEELQTLFGESSENPM
                     YDCYPITPKNASFFQELAQQDLRFDVYDYFLECEEDTD"
     gene            complement(743..>2704)
                     /locus_tag="PKZ53_11695"
     CDS             complement(743..>2704)
                     /locus_tag="PKZ53_11695"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNJ41145.1"
                     /translation="SPSDLPGMVRFGSWIGGDRDGNPFVTAARTEDALRMARGVILEY
                     YIRQIHELLKYLSSSVGQVDASTELTSAVAAYRERFKQVNPANPARSSHELYRCFLDF
                     VLHRLDATRTTPTHIDAYADTSEFREDLQLLRRSLAANRGERIARLELEPLLRQVQTF
                     GFHLVTLDIRQHGRVHSTAVKELSQGNQFHLEASTALPAPSATTLELLETLRTIARLK
                     SSYPSETMERYIISGSESEADILNCRWLCELGGIRTGGNPARGDQGLMPVPLFESIED
                     LRNSPRICRALWTSPAYQSLLNAWGREQEVMLGYSDSNKDGGMMTSSWELYKAHRELH
                     RVAGETGVKLRLFHGRGGTVGRGGGPTHRAIISQPVGAFTGHIKITEQGEVLNWKYSD
                     PILAERNLELMIAASLEALTRTGTPSTPPGPEIEAAMDTMSGLAFEFYRTHIVENPDI
                     LTYFEEATPVSELQHARIGSRPARRSERRGLQDLRAIPWVFGWMQSRHVVPGWFGVGT
                     ALEQFARSQPENLTLLRQMMQDFVLFTDMIRNVEMSLAKADLNIARRYAALVSNRDIR
                     ERVFSLIETEFHRTRQMVLDITEQSRLLEANPVLARSIQLRNPYVDPMSFLQIELLRR
                     KRAGEASEELNYALATTINGIAAGLRNTG"
BASE COUNT          673 a          726 c          687 g          618 t
ORIGIN      
        1 ccgaggtcac aactgatttt gacaatattc cacgaccaca aggcgggaag tacgatattg
       61 gcgcatttga atttttcacc gcgctcaaac ccgagtctca ttctgacgaa atcacccctg
      121 agttgatccc acaggacggt cttgaaaact ccgacacaga tcggtaatga caaatcagcc
      181 cggtttgaca acctgaccca tttacccaaa cccgcaggag acaattcttg cgggtttgat
      241 tttttggcct ttcccagtac gaatgagaaa aaccaccgat gaaacaagaa ctttccattt
      301 ttgatcacat tcgggccagc gatatggagc atctcacttt tgaaaaatat aacaagtgtg
      361 gctcctactt ggtaagtttg atccaactgc cagagaacca aaccagcaaa tcctaaacgg
      421 aaattgagcc ataaaaccga ggcaacgatg cacaaaataa tccggtcgat caggtggtat
      481 gacaaacaag gtgaagattt caagggggag cttgaacttc gtgaaattcc agttgaggaa
      541 ctccaaactt tgtttggtga atccagtgaa aatccgatgt acgattgcta cccaattact
      601 ccgaagaatg cctctttctt tcaagaactt gcccaacagg atctgagatt cgatgtgtat
      661 gactatttct tagaatgcga agaagacaca gattgaaagg gtactttcac gatcatcaat
      721 cccggctggt ctttgatcct tgtcaacctg tgtttctcaa cccggcggca attccattga
      781 tggtggttgc cagggcatag tttagttctt cgcttgcctc accagcccgt ttgcggcgca
      841 ggagttcaat ttggagaaaa ctcatcgggt caacatacgg attacgcaac tggatcgaac
      901 gagccagaac cgggtttgct tcgagcaacc ggctttgctc agtgatgtcg agcaccatct
      961 ggcgtgtgcg gtgaaattcg gtttcgatca acgaaaaaac ccgttcccgg atgtcacggt
     1021 ttgaaaccag ggcggcatac cggcgggcaa tgtttaaatc ggctttggcc aggctcattt
     1081 ctacgttgcg gatcatatcg gtaaacaaca caaaatcctg catcatctgg cgcaacaagg
     1141 tcagattctc tggttgtgag cgagcaaact gttcaagcgc cgttccaacc ccaaaccagc
     1201 cgggcacaac atggcggctt tgcatccagc caaaaaccca gggaatggct cgcaaatcct
     1261 gtaagccgcg ccgttcactt cggcgtgccg ggcgcgagcc aatccgtgcg tgttgtaatt
     1321 cgctcaccgg ggtggcttct tcaaagtagg tcaaaatatc aggattttcg acaatgtggg
     1381 ttcggtaaaa ctcaaacgcc agccccgaca tagtatccat tgccgcctca atttcagggc
     1441 ccggtggtgt gctgggggtt ccggttcggg tcagggcttc taacgaagcg gcaatcatca
     1501 attccaggtt ccgttcggcc aggattggat ccgaatattt ccagttgaga acttcaccct
     1561 gttcagtaat tttgatgtgt ccggtaaagg caccgaccgg ctgtgaaata atcgcccgat
     1621 gcgttggacc gccgccgcgc cccaccgtgc cgccgcgtcc gtgaaacaac cggagcttga
     1681 cgccggtttc accagccacc cgatgcaatt cacgatgggc tttatagagt tcccaggaac
     1741 tggtcatcat gccgccgtct ttgtttgaat cagaatagcc gagcatgact tcctgttcgc
     1801 gtccccaggc gttgagcaac gattgatagg ccggagacgt ccagagcgcc cggcagattc
     1861 gcgggctgtt gcgcaagtcc tcaattgatt caaacagtgg aaccggcatc aatccctggt
     1921 cgccccgcgc tggattacca ccggttcgaa tgcctcccag ttcgcagagc caccggcaat
     1981 ttaaaatatc agcctccgac tcagaaccgc tgatgatata gcgttccatg gtttccgaag
     2041 gataactgga tttgagacgg gcaatcgtgc ggagcgtttc cagaagctct agcgttgttg
     2101 ccgatggggc cggtagcgcg gtggacgctt caagatggaa ctgattcccc tgggagagtt
     2161 ctttgaccgc ggttgaatgg acccgcccgt gctgacggat atccagcgtc accaggtgaa
     2221 acccaaaggt ttgaacctgc ctgagcagcg gctccagttc cagtcgggcg attcgctcgc
     2281 cacgattggc ggccaggctg cgccgcaaaa gctgcagatc ctcacgaaat tcgctggtgt
     2341 cagcatacgc atcaatatga gtcggtgtgg tgcgcgttgc gtccagccga tggagcacaa
     2401 aatcaagaaa acaccggtag agttcgtgcg acgaacgagc cgggttggcc ggattaacct
     2461 gtttaaatcg ttcacgatac gccgccactg ccgaagtcaa ctctgtcgag gcatcaacct
     2521 gcccaaccga cgaactcaaa tattttaaaa gttcatggat ctggcgaatg taatactcca
     2581 aaatcacgcc acgggccatc cgtagcgcat cttcggtgcg ggctgccgtg acaaacgggt
     2641 ttccatcccg gtcgccgcca atccaggacc caaaccggac catccctggt aagtccgaag
     2701 gtga
//
LOCUS       DAOQDO010000696         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31775_length_2702_cov_3.681526, whole genome shotgun sequence.
ACCESSION   DAOQDO010000696 DAOQDO010000000
VERSION     DAOQDO010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31775_length_2702_cov_3.681526"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..2262
                     /locus_tag="PKZ53_11700"
     CDS             211..2262
                     /locus_tag="PKZ53_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ41146.1"
                     /translation="MLAPGLVLQDRYRIVRQLGKGGMGAVYEAQHVKLGHTVAVKETF
                     FSEDHLTKAFEREARLLAGLSHPALPKVSDYFSQDSGHFLVMEFIPGKDLSAYLAEHG
                     TALPLQQVLHWADHLLDALEYLHNQQPPVIHRDIKPANLKVTAEGRIFLLDFGLAKGL
                     HTQMSQVPGSSGASVQAYTPGYAPVEQMQGSGTDARSDVFSLSATLYHLMTGAPPVDA
                     LTRAMKVAKRLPDPLPLASEINWMIPVSVARVLQQALDLDEDLRPTTAADFRRMLRQA
                     STGSIEQPGTVLVAGDATNPGASFHNDVTRQASQPINWSESQTVVDPSKSVPPVMSPP
                     ESFQTRPVAPPPAHPTLPPTVHISPPEPERPAPESSKKWMLPVLALVMLVLVVGVIGA
                     RMMWQARKNNLASSSAPTQAEKKNKPSTPSPKKSDETPMVVATPLSPTFTNRIGMEFV
                     LISPGSFLMGSLESEADRETDEGPQYKVTISQPFYLGKYEVTQAEWKVVMEDNPSEFP
                     GERLPVDSVSWDDCQEFLKKLNARNDGYTYRLPSEAEWEYACRAGSKTPFSFGEKVTM
                     KQVNFSGNFPYSDDPETDGQGKPVSVGSYPANAWGLYEMHGNVREWCQDWLQVDYTDA
                     PTDGRPWVSGPETQYRVLRGGAWNVNARDCRSATRSGNTPDATDNSYGLRVVAEKVEK
                     N"
BASE COUNT          663 a          714 c          721 g          604 t
ORIGIN      
        1 cagttgtcat cgtagggatt cctccaccgt aatatgaccc aaacttgcca caaaccacct
       61 caacccgcaa ccacgaaaga atgtgaagga aatcgctgct ccagtttttt tccgttcagt
      121 gaccttccca tcgggcggtt gaatcgcgta ttgttcgccc catcaaatcg aacaggctgt
      181 tctatcccaa gtttttgaat tttctgatct atgcttgcac ctggacttgt tttacaggat
      241 cgctatcgga ttgtgcgtca gttagggaaa ggcggaatgg gcgcggtcta tgaagcccaa
      301 cacgtcaaac tcggccacac cgtggcggtc aaagaaacct ttttttccga agaccacctg
      361 accaaagcgt ttgagcgcga agctcgactg ctggccgggc tgagccaccc ggcactcccc
      421 aaggtcagtg attattttag tcaggacagc ggccacttcc tggtcatgga gtttattcca
      481 ggcaaggatc tgagcgcgta tctggccgaa cacggtacag cattgccgct tcaacaagtg
      541 ttgcactggg ctgatcattt gctggacgcg ctggagtatt tgcataacca gcaaccgccg
      601 gtgattcatc gggatatcaa acccgccaac ttaaaagtaa ccgcagaagg gcggattttt
      661 ctgctggatt tcgggctggc aaaaggactt catacccaga tgtcacaggt ccctggatca
      721 agcggggcca gcgttcaggc atacaccccc ggctatgcgc cagttgaaca aatgcagggc
      781 agtggcaccg atgcccggag cgatgtgttt tcactcagcg ccacgctcta tcaccttatg
      841 accggggccc cgccggtgga tgcgctcacc cgtgccatga aggttgccaa acggttacct
      901 gatccgcttc cactggccag cgagatcaac tggatgatcc cggtgtcggt ggccagggtg
      961 ttgcagcagg cacttgatct ggatgaagac ctgcgtccga caacagcggc agattttcgg
     1021 cggatgctgc gccaggcaag tactggcagc attgaacaac ccggaaccgt cctggtcgcg
     1081 ggcgacgcga ccaatccagg cgcttcattt cacaatgatg tgaccagaca ggcatctcaa
     1141 ccgatcaact ggagtgaatc acagacagtg gttgatccca gcaaatcagt tccgccggtg
     1201 atgtccccgc cagagagttt tcagacgcgc ccggtggcgc cgccgccagc tcatccaacc
     1261 ttgccgccaa ccgttcatat ttcaccgccc gagccggaac gtccagcgcc ggagtcttcg
     1321 aaaaaatgga tgcttccggt actggcgctg gtgatgctgg tgctggttgt cggagtgatt
     1381 ggggccagaa tgatgtggca ggcacggaag aacaatctgg cctcctcgtc agctcccacc
     1441 caggctgaaa agaaaaataa accgtccaca ccctctccaa aaaagagtga cgagacgcca
     1501 atggtggtag cgacaccgct cagtccaact ttcacaaacc gaattggtat ggagtttgtc
     1561 ttgatttcac cgggaagttt tttgatgggg tctctggaaa gtgaagcgga ccgcgaaaca
     1621 gatgaaggcc cacagtacaa agtgacgatt tcccagccgt tttatctggg caaatatgaa
     1681 gtgacccagg ctgagtggaa agtggtcatg gaagacaatc cgtcagaatt tccaggcgag
     1741 cgattgccgg tggatagtgt ttcctgggat gattgccagg aattcttaaa aaagctcaac
     1801 gcccgcaatg atggctatac ctatcggctg cccagcgagg ctgagtggga atatgcctgt
     1861 cgggcgggaa gtaaaacacc cttttcgttt ggcgaaaagg tgacgatgaa gcaggtaaac
     1921 tttagtggaa atttcccata tagcgacgac ccggaaacgg atggccaggg gaaacccgtt
     1981 tcggtgggaa gttacccggc caatgcctgg ggactctacg aaatgcacgg taacgttcgc
     2041 gaatggtgtc aggactggct acaagtagat tacaccgatg ccccgaccga tggccgtccc
     2101 tgggtttctg gaccagaaac ccagtatcgt gtactgcgcg gcggagcgtg gaatgtcaac
     2161 gcccgtgatt gtcgctcggc cacccggagc ggaaacactc cggatgctac ggataactca
     2221 tacgggctgc gggttgtagc tgaaaaggtc gagaagaatt aagccagaag gtttgagcct
     2281 tcctttcttg agcttgtgga ataataccaa ttagggttca gggtatcggg ttcagggttc
     2341 agggaaatac aattttttca atattttagc cttcccgtaa tacgaggatt tcattgcaaa
     2401 atggaataag tcatggaaca aactgagcgc caaatccatt tactctgtca ccaaattgcc
     2461 gaaaaatttc atccagaaca aatcattctc tttggatcac acacttgcgg atatcttcct
     2521 ctgaaccttg aaccctgaac cccgaaccct gatttgcttt atggaagctt cgaaacctga
     2581 cgccatccaa cctgcactta tggacgctcc gaagtcggag gcgctggcgc cgtcggctct
     2641 ggtgattgaa caaattgagt ttcaggaaca acttgccgtt caatccgtgc cggaaacgct
     2701 cc
//
LOCUS       DAOQDO010000697         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31792_length_2701_cov_7.691610, whole genome shotgun sequence.
ACCESSION   DAOQDO010000697 DAOQDO010000000
VERSION     DAOQDO010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31792_length_2701_cov_7.691610"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKZ53_11705"
     CDS             <1..730
                     /locus_tag="PKZ53_11705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ41147.1"
                     /translation="RARTAYRLGQMRCASAIETLTTALRDPSQEVRLMAIWALTEIGD
                     DRAVSPIVVSLADANGWELMHAANCLLGMTGELTLPLLDLLRASGPKRDRRERISATV
                     LDLLSEFGQRARNRLNPRACRQAADDLLKSDSVDIRARAVRALTALGIESHDELEVIL
                     RCLKDPAWEVRAVTARALGEMQVLDAIPLLAQAVSDEAYWVRHNAAFALKQFGEFGYE
                     ALDHLQRSQDRFAREMARQVMEQG"
     gene            888..2537
                     /locus_tag="PKZ53_11710"
     CDS             888..2537
                     /locus_tag="PKZ53_11710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ41148.1"
                     /translation="MENLGLRTENFPHRDTSFAGRFSRFGGWLFLLSPLILGVVFFAL
                     ELSLGSRQATSSAFSRAIHDLFVVLLGFILVAGLIRMIQMIVSVVQIRVYSLSQQLGQ
                     GWLLPYSLRVPPISLIVWVHNEAGTITERMERLLKLEYPTFELVVVNDGSTDATQQIL
                     QSSFDLHPVNKVICRTLPTRTDGNVYTSALYPNLTVVDKPWSGRGDSLNFALNLTQYP
                     LVLAEDSDTILQSRALVRLAKGFIEDHTNTVAVSSLAALTGDINLEIPDSEIGLGRNQ
                     DSSESPDVPVVESSPITRYAPTVSIFGATFAEDDLSTWIQNLQRVDRMSAFHSSWLAR
                     NWLGSVPVSGGIPRLLRKSDVVAAGGYDPMSEEVSFNLLLHIQYQDKRKLPRRVVFLS
                     DILARIEVPEDLNVIGQAQRNWQRLAFRSLITHWKLIFKSHGDFWWRSLSYPVFFLID
                     ILGPVIELIGFGLVFVMGVLGAIEPSEMMFFIFAMMTFALVQSIGAVLSEEFSSRSIR
                     KPGRLLKLILVASTHAIGYRQMSAYWKLRGIIDYALGKGTY"
BASE COUNT          620 a          639 c          720 g          722 t
ORIGIN      
        1 gcgggcacgc actgcctatc ggcttgggca aatgcgatgt gcgtcggcca ttgaaacttt
       61 gacaaccgcg ttgcgtgatc catctcaaga ggttcggttg atggcgatct gggcattgac
      121 tgaaatcgga gatgatcgtg cggtttcccc gattgtcgtt tcactggctg atgccaatgg
      181 ctgggaattg atgcatgccg caaactgttt gcttggaatg actggcgaat taacgcttcc
      241 gttgcttgat ctcctgcggg cttccggtcc caaacgtgat cggcgcgagc ggatttcagc
      301 cacggtcctt gatttgctgt ctgaatttgg tcaacgggcc cgcaatcggc tcaacccacg
      361 tgcctgtcgc caggcggcgg atgatttact caagtctgac tcggttgata ttcgcgcccg
      421 ggcggtccga gccttaaccg cactcgggat cgaatcccat gatgaacttg aggtcattct
      481 gcgctgttta aaagatccag cctgggaagt tcgggctgta acggcacgtg ctcttggcga
      541 aatgcaggtg ctcgacgcaa ttccgttgct agctcaggcg gtgtctgatg aagcatattg
      601 ggtccggcat aatgccgctt ttgccttaaa acaatttggt gaatttggat acgaagcact
      661 ggaccacctt caacgaagtc aagatcggtt tgctcgcgaa atggccaggc aggtaatgga
      721 gcagggatag ctgagcaatc gggaaacact tcaatctgtt tgaagaccgc tccccctcgt
      781 cacccgaacc caggtactcg ccaccacgtg ccacttaaca atggttcctc tcactaccga
      841 gttcttttga ccgacggaaa actcatttcg tcaaaccata cttgcccatg gaaaatcttg
      901 gactgcgaac tgaaaatttt cctcaccggg ataccagctt tgccggacgg tttagccggt
      961 tcggtggctg gttgtttctg ttaagcccgc tgattttggg agtggtattt tttgcactgg
     1021 aactcagttt ggggagccga caggcaacca gttcagcctt ctcccgtgct attcatgacc
     1081 tgtttgttgt cttgcttggc tttatcctgg ttgctggcct gatccgaatg atccagatga
     1141 tcgtgtcagt ggttcaaatc cgggtctata gcctgagcca gcaacttggt cagggatggt
     1201 tattgcccta ttcactccgt gtcccgccta tttcactcat tgtctgggtt cataatgaag
     1261 cgggaaccat tactgaacgg atggagcgtc tgctcaagct tgaatatccc acctttgaac
     1321 tggtcgtggt taatgatggg tcaactgatg cgacacagca gattctgcaa tcatcatttg
     1381 atctgcaccc ggtgaataag gtgatttgtc ggactctacc aacgcgaact gatggcaatg
     1441 tgtacacgtc agcactttac ccaaacctga ccgtcgttga taaaccctgg agtgggcgtg
     1501 gtgattcact gaactttgcg ctgaacctga ctcaatatcc tctggtgctg gctgaagact
     1561 cagatacaat tttgcagtcc cgggcgctgg tcagattggc aaaagggttt atcgaagacc
     1621 acaccaacac ggtggcggta agcagcctgg ctgctctgac tggggatatc aacctggaga
     1681 taccggattc agaaatcggg ctgggccgca atcaggactc ttcagaatca cctgatgtcc
     1741 cggtggttga gtcgtcaccc atcacacgat atgccccaac cgtcagcatt tttggggcta
     1801 cgtttgccga ggacgacctt tcaacctgga tccagaacct gcaacgcgtt gaccgaatga
     1861 gcgcttttca ttcaagctgg ctcgctcgga actggttggg tagtgttccc gtctcaggtg
     1921 gcattcctcg attgctccgc aagtcagacg ttgtggctgc cggtgggtat gatccaatga
     1981 gcgaggaagt gagctttaac ctgctgctgc atattcagta tcaagacaaa cgcaagctgc
     2041 cgcgtcgggt cgtgttcctc tctgatattc tggccaggat tgaagttcca gaggatttaa
     2101 atgtcattgg acaggcccaa cgaaactggc agcgcctggc ctttcgatcc ctgataactc
     2161 attggaagtt aatttttaaa tctcatggcg atttttggtg gcggagcctg tcctacccgg
     2221 tctttttcct gatcgatatt ttgggtccag tgattgaatt gattgggttt gggctggttt
     2281 ttgtgatggg tgtgttggga gcgattgaac catcagaaat gatgttcttt atttttgcga
     2341 tgatgacatt tgccctggtc cagtcaattg gcgcggtctt aagtgaagaa ttttcaagtc
     2401 gttcgattcg aaaaccagga agattactga agttaatcct tgtagcctca acgcatgcga
     2461 ttggataccg ccagatgtcc gcttactgga agctccgggg aatcattgat tatgcgttgg
     2521 gaaaaggaac ttactgaatg aagaattgag aatgaagaat gaagaattga gaatgaagaa
     2581 tgaagaatga agaatgaaga atgaagaatg aagaatgaag aatgaagaat tgagaatgac
     2641 ctcaattttg cccaacatag caccacctgg ctcacatatc agtgccggtc agttcgggat
     2701 c
//
LOCUS       DAOQDO010000698         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31801_length_2701_cov_3.221466, whole genome shotgun sequence.
ACCESSION   DAOQDO010000698 DAOQDO010000000
VERSION     DAOQDO010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31801_length_2701_cov_3.221466"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..>2701
                     /locus_tag="PKZ53_11715"
     CDS             287..>2701
                     /locus_tag="PKZ53_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41149.1"
                     /translation="MTLDQLEQEYQFKLSQVTLVAEINITVEQWESLAQELRELVARR
                     KELFGSEKKALSILEKHPNCVLVFLVAQGIFSYRSGDFWTGVATRLGLETKAHCQSVL
                     GEIADNQFKKLDLPVLDKELGALRFVAPILFHGGIPRDYLPDFFSSFLKGNTSGFIDP
                     GSLIEEWLLLTATMPVLKPVRRFLEYGGYLARNFVARCIETHRYFLQHRRLPLPEEAG
                     LPQWILDAYREWNTKSPAVTTGNTPHLIFRRPEFELDPWEPQIWVILPEQQMPFDQRP
                     VLEWKMIADENPSLICVPSCQRGETGYHSQLITERIESPAREYRIHLNFNGLSKSWTI
                     PGISVTQPFLVFDGATRRLIRWEHELPASPVWIVYQENQECVSQKGYCLEKGSQFWDG
                     WQGFQVECWDLTAARQIKIGHTSFGVGRKPVPQFIEGTLARYQPRATEPPFYVGTPPA
                     LFIPINSKAEVYQFLQPSMLRIGRGNRPGTFIQTIQFQYVYDEVLRGVKIDLTSNKPH
                     GLAHNEFGEIEVSFRGKSLKQDFLGVFAFISQLNISGNDFCQLPNTDGSYASRTIQLD
                     IDPQVTCTLEIAGTRLIPEKRKDGQITFEVAEPGGELVLRRGYQFGVSQIQFRVPCPQ
                     LYWKLTSNGIELSRNRQFCQSALWFEQQIDLKIEVGVTASIQSFEVWLHLIDETDQVV
                     IQELRGKGPTQGGIQFHLSEAYDTVRAHVSSQMCFRLVLNPDFVIGEGDSHQISVLTL
                     SRPELVEVVADPEPWAEIVENLISVEIQPPSLTTNLLEPVAEEKIPSKDPILEIKEWC
                     ELCREGK"
BASE COUNT          723 a          602 c          673 g          703 t
ORIGIN      
        1 atgccgaatt tcccaaaggg gtttctgacg agatcaaacg ggcaatttca gaaaatgcca
       61 gtatcctgaa gttcaagaac aaaacctggg agtaagagaa ctaaaagata tcggcttcga
      121 ccagagttgg tgatatgcca ggcagttttg ggataggttt taagtctgat aatataatgc
      181 cagtttgtag tcagcttttc aggtctgaag ccggtgccag ttgccccaat gtttcaccga
      241 tcaaatctgg tctcaaccac ctttttgtca aaaatcgagt gcgatgatga cgctagatca
      301 actggagcaa gagtatcagt ttaaactctc acaagtaacg ctcgttgctg aaatcaacat
      361 caccgttgag caatgggagt cgctggctca ggaattgcgg gaacttgttg ccagacgcaa
      421 agaactcttc gggtcagaaa aaaaagccct gtctattctg gagaagcatc caaactgcgt
      481 gcttgttttt ttggttgccc agggcatttt ttcatatcgg agtggtgatt tttggacggg
      541 agtggcaacc aggctgggtc tggaaaccaa ggcgcactgc cagtcagtcc tcggtgaaat
      601 agctgataat cagtttaaaa aattggattt accggtactc gacaaggaac tgggagcgct
      661 gaggtttgtg gccccaatcc tctttcatgg tggtattccc cgcgactatc tgcccgattt
      721 cttttcctca tttctcaaag ggaatacatc aggttttatt gacccaggtt ccttgatcga
      781 ggagtggtta cttttaactg cgactatgcc ggtcttaaag ccagtgcgac ggtttttgga
      841 gtatggggga tatttagcca gaaatttcgt cgcccgctgt attgaaactc atcggtactt
      901 tttgcaacac cgaagacttc cacttccaga agaagctgga cttccgcaat ggattttgga
      961 tgcgtatcga gaatggaata ccaaaagccc tgcggtgaca accgggaata ctccacacct
     1021 gatctttcgg cgtccggaat ttgaattaga cccctgggaa cctcagattt gggtgatttt
     1081 gcctgaacaa caaatgccct ttgaccagcg gcccgtcctg gaatggaaaa tgatcgctga
     1141 cgagaatcca tctttgatct gcgtgccttc gtgtcaaaga ggagaaactg ggtatcactc
     1201 acagcttata actgaacgaa ttgaatcacc cgccagggaa taccgcatcc acttgaattt
     1261 caacggatta tcaaaatcgt ggacgattcc tggaatttcc gtaacccaac cgttcctggt
     1321 ttttgatgga gcgacccggc ggttgattcg atgggaacat gagttgccgg ccagtccggt
     1381 gtggattgtc taccaggaaa accaggaatg tgtgagccag aaggggtact gcttagaaaa
     1441 gggcagtcag ttttgggatg gatggcaggg ctttcaagtt gaatgttggg atttgacggc
     1501 agctcgacag ataaaaatcg gccacaccag ctttggtgtt ggccgcaaac ctgtccctca
     1561 gttcatcgaa ggaactctgg ccaggtatca acccagagca actgagccac cattctatgt
     1621 cggaactcca ccagctttat ttattccgat caactcaaag gccgaagtct atcagttcct
     1681 tcaaccaagt atgctccgaa tcgggcgcgg caaccgacca ggtacgttca tacaaaccat
     1741 tcaatttcaa tatgtttatg atgaagtatt acgaggcgtg aaaattgacc tgacttcaaa
     1801 taaaccacat gggctggctc acaatgagtt cggtgaaatt gaagtttcgt ttcgagggaa
     1861 atcactgaag caagattttc tgggagtgtt tgcctttatt tcccagttga atatttctgg
     1921 aaacgacttt tgccagctac ctaatacaga tgggagctac gcatcacgaa caatccaact
     1981 ggacatagat cctcaggtaa cctgcacttt agaaattgct ggaacccgac tgattccaga
     2041 aaaaaggaag gatgggcaga ttacatttga agtggcagaa ccaggtggag agcttgtact
     2101 gcgtcgaggc tatcaatttg gagtcagtca aatccaattc agggtgccct gtccccagct
     2161 ttattggaaa ctgaccagca acggaattga actctctcga aatcgccagt tttgtcagtc
     2221 agccctgtgg tttgaacaac agatagacct gaaaatcgaa gtcggcgtca ccgcaagtat
     2281 ccaatcattt gaggtttggt tacacctcat agacgagacc gatcaggttg ttattcagga
     2341 attgcgtggc aaaggcccaa ctcaaggggg gatccagttt catttgtctg aagcttatga
     2401 cacggtgaga gcccacgttt catcacaaat gtgttttcgg ctggtattga atccagactt
     2461 tgtgattggt gagggtgact cacatcagat ttcggtgttg acactctcca ggccagagct
     2521 tgttgaggtc gttgctgacc cggaaccctg ggcggagatc gtggaaaatt tgatttcggt
     2581 tgaaattcaa ccaccttctc tgactaccaa tctgcttgag ccggtggctg aggagaagat
     2641 cccatccaaa gacccgatcc tggaaatcaa agaatggtgc gagctttgtc gagaaggaaa
     2701 g
//
LOCUS       DAOQDO010000699         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31806_length_2701_cov_2.733938, whole genome shotgun sequence.
ACCESSION   DAOQDO010000699 DAOQDO010000000
VERSION     DAOQDO010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31806_length_2701_cov_2.733938"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..683
                     /locus_tag="PKZ53_11720"
     CDS             90..683
                     /locus_tag="PKZ53_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNJ41150.1"
                     /translation="MDRLKAIQDTIQTSIELKQRFFAEHSETVLKAARQTAEAIRRGG
                     KILIFGNGGSAADAQHFAAELVHRLDHTRPERPGIPALALTTDSSTITAIANDASFTQ
                     VFSRQIVALGRAEDVAIGISTSGNSPNIVEAIRQANQMGMVTIGLLGKDGGQLRELVA
                     IPLIVPHHITARVQEVHLIIEHVLCELIEEELFPESQ"
     gene            1090..2565
                     /locus_tag="PKZ53_11725"
     CDS             1090..2565
                     /locus_tag="PKZ53_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNJ41151.1"
                     /translation="MFELFDSSLSTGTQIILILSLLIACGFEFVNGFHDTANAVATVI
                     YTNTLKPWIAVIWSGLCNFAGVLLGGISVAMGIIYLLPSELLVNKGTGAGLAMVMALL
                     LAAILWNLGTWYYGLPASSSHTLIGAILGVGLANSMLPGHTFGDGVNWGKATEIGLSL
                     LVSPLTGFSVAFLLLWLSKKLIPNEKLYTVPTEAEPPPLWIRLILIVTCTGVSFAHGS
                     NDGQKGVGLIMLILIGILPASYALNATLTQTQLTQTVEAARQLDLVLQRYDDSKPLLQ
                     IGFAPDSSGTPTPNTPTLGTPISNLRAELADIQTVLAGKNSVKEIAKDSRWTVRKDIL
                     LVVSSLTKLEKGGKIKFSEEDSKLVKKNLSVLRGATDYAPLWVFMAVAISLGIGTTVG
                     WKRIVITVGEKIGKSHLTYAQGASAELVAMSTIGLASTFGLPVSTTHVLSSGVAGTMV
                     AQKAGLQAATVRNIALAWILTLPVSICLSAVLFLIFHSLFA"
BASE COUNT          654 a          700 c          646 g          701 t
ORIGIN      
        1 attgatttgg taaggtgctg agtttccctc tttctgaggg aaatccatct taatcagatt
       61 catggctgag agggcaagag gttctttgct tggacagact aaaagcaatt caagacacaa
      121 ttcaaaccag tatcgaactc aaacaacgtt tttttgctga acactccgaa accgttctga
      181 aagccgcccg gcaaacggcg gaggcaattc gacgcggcgg aaaaattttg atcttcggca
      241 atggcggaag cgcggcagat gcccaacact ttgccgccga actggttcac cgactcgacc
      301 acacccgccc ggaacgacct gggattccag ccctggcgct gacgaccgat tcctcaacga
      361 ttacagccat tgccaacgat gcctcgttta ctcaggtatt tagccgccaa attgtggccc
      421 tggggcgagc cgaagatgtg gcaattggga tttccaccag tgggaattcc cccaacatcg
      481 tcgaagccat tcgacaagcc aatcaaatgg gcatggtcac catcggcctg cttggcaaag
      541 acggaggcca gttgcgcgaa ctcgtagcta tccccttgat tgtgccccac catatcacgg
      601 cccgtgttca ggaagtccac ctcatcattg agcatgtgtt gtgtgaactg attgaggaag
      661 agctttttcc ggagagtcag tagtcagtcg tcaggagtca gcagtcagat gttaggattt
      721 tgaattgcct gtcaagtcta tctggttcta tttgagttgg ttccgaacaa ttcttttcaa
      781 gaattccctg gattcaacga tttgggtttt atctactgac aactgactca tcaacgactg
      841 actcatcaac aattgactcc cttgtgatta acctcaattt aacatcctgc acacatactg
      901 cgggcaaatc catttctcct gatccaaaat ccggctctta tgccccatgc cttccacaca
      961 agcactgaag ttctctcact catctccctt gtggctgttg attttcgtca gaaaactccc
     1021 ctgaaaggaa tgtttcccat ttccagactg gctttgccct gcggggtata gccagcggaa
     1081 tccgatttta tgtttgaact ctttgactcc tcgctcagca ccggtaccca gattattctt
     1141 attttgtcgt tgctcattgc ctgcggtttc gaattcgtca atgggtttca cgacacagcc
     1201 aatgcagtgg caactgtcat ttacaccaac actttaaaac cctggattgc ggtcatctgg
     1261 tcaggacttt gtaactttgc aggtgtgctg ctgggaggaa tctcagttgc catgggaatt
     1321 atctatctgc tgccgtctga gttactggtc aacaaaggta cgggggccgg tctggcgatg
     1381 gtgatggcgc tgttgctggc ggctattttg tggaatctgg gtacatggta ctacgggttg
     1441 ccggcctcaa gctcacatac cttaattggt gcaattttgg gagtcggatt ggccaattca
     1501 atgcttcctg gccatacctt cggcgatggc gttaactggg gcaaagccac cgaaattgga
     1561 ctttcgctgc tggtttcacc attgactgga ttttcagttg cctttctgct gctgtggctt
     1621 tccaaaaagc tcattccgaa cgaaaaactg tataccgttc caactgaagc cgaaccgcca
     1681 ccgctgtgga tccgcctgat tttgattgtg acctgcactg gcgtcagctt tgcccacgga
     1741 tcaaatgatg gccaaaaagg tgtcgggttg attatgttga tcctgattgg gatcctcccg
     1801 gccagctatg cactcaatgc cacgctcacc cagacgcaac tgacccagac cgtcgaagct
     1861 gcccgtcagc ttgatttagt cttacagcgc tatgacgact caaaaccgct gcttcaaatt
     1921 gggtttgccc cagacagcag tggaacaccc acaccaaaca caccaactct gggaactccg
     1981 atttctaatc tccgggcaga actggctgat attcaaaccg tgctggctgg aaaaaactca
     2041 gtcaaagaaa tcgctaagga ttcccggtgg acggttcgca aagatatttt gctggtagtg
     2101 tcgagtctga ccaaactgga aaaaggcggc aaaattaaat tctcagaaga agacagcaaa
     2161 ctggtgaaaa agaatctgtc cgtgcttcga ggcgcaacag attatgcccc actatgggtc
     2221 tttatggcgg tggcgatttc acttggcatc ggaacgacgg tcggctggaa acgcatcgtc
     2281 attaccgtcg gtgaaaaaat cggaaagagc cacctgacct atgcccaggg tgccagtgcc
     2341 gaactggtcg ccatgtccac cattggtctg gcatcaacct tcggattgcc cgtcagcacc
     2401 acccacgtct tgtcatctgg ggttgcaggc acgatggtgg cccaaaaagc gggactccag
     2461 gcagctacgg tgcgcaacat cgccctggcc tggattctga ccttacccgt ttcaatctgt
     2521 ttatcggctg tgctgttttt gatcttccat tccctcttcg catagacact gaatgatgcc
     2581 agtcagtcgt tgatgagtca gtagtcagta gtcagtagat aaaacccgga tattgcagtg
     2641 tgtaggattc ttgaaaagaa ttattcataa ctgactcaaa tagaaccaaa tacacttgac
     2701 a
//
LOCUS       DAOQDO010000700         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31808_length_2701_cov_2.708617, whole genome shotgun sequence.
ACCESSION   DAOQDO010000700 DAOQDO010000000
VERSION     DAOQDO010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31808_length_2701_cov_2.708617"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1435)
                     /locus_tag="PKZ53_11730"
     CDS             complement(47..1435)
                     /locus_tag="PKZ53_11730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNJ41152.1"
                     /translation="MNTQNLDSVSKERVLNSLFQESYSPEQFYEAICQGSPDVIVIVD
                     RQGNMVFVNDRSYELLGYAPEELIGKNVECLVPPQFTEHARLRADYHRSPEPHIMGNR
                     PILSAWHKSGVEIPVDIALSPLPEIGGRVGMVLAAIRNALPRWSAYQEQYVQSVALNA
                     AANGIIITDCHGVICWANPSVSRMTGYSLKELVGHHTRILKSGQHEPSFYKDIWDTVL
                     AGKTWFGSITNKRKDGSLYYEEQHIAPVISEKGEVTHFIAIKQDITSRKIAELALKTA
                     NEELNLKIAQIESLHYQLREQAIRDPLTGLFNRRYLEETMVRERARVRRERKTLSLVL
                     IDVDNFKQVNDTAGHAAGDLVLKALAELLLRTTRTSDVACRMGGDEFLVLLPGASLKI
                     ATQKAEHWRTAFYQDQHEILSQTGHIHCSLSLGVTELKTSDESTDSLLNRADEYLYLA
                     KKQGRNCVVSSP"
     gene            1831..2481
                     /locus_tag="PKZ53_11735"
     CDS             1831..2481
                     /locus_tag="PKZ53_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNJ41153.1"
                     /translation="MTEQSHDHHPYTPAEELANWLSHGAGFVGSLIALPILVASALGQ
                     SNLQIVAVSVFAASMMLLYLASTIYHVLPPSKHKHLFRIVDHAAIYVLIAGTYTPFTL
                     GVLRGAWGWTLFGIVWSMAIAGIVFKALGGIRFDKLSTALYLAMGWLVVIAIKPLITL
                     VPMAGLVWLFVGGIFYTGGVAFYAATRLKYHHFLWHLCVLAGTFCHFFAVLWYSQG"
BASE COUNT          615 a          683 c          677 g          726 t
ORIGIN      
        1 tcatccctca tcccttcgat tgccccaggc ccttaaccca agggttttat ggcgacgaga
       61 caacacagtt tcgcccctgt tttttggcca ggtagaggta ttcatccgcc cggttgagca
      121 atgaatcagt tgactcatct gaggttttca gttcagtcac tcccagggaa agcgaacagt
      181 ggatgtgacc tgtctggctc aagatttcgt gctgatcttg ataaaaggcg gtgcgccagt
      241 gctctgcctt ttgagtcgca attttgagcg aagcacccgg caataacacc agaaattcgt
      301 cgccgcccat tcggcaggca acatcactgg ttcgggtagt tcgaagcaac agttccgcca
      361 gcgctttgag aaccaggtct ccggcagcat gacccgctgt atcgttgacc tgtttgaaat
      421 tgtctacgtc aatcagcacc aacgacaggg tttttcgttc gcgccggacc cgagcccgtt
      481 cacgaaccat cgtttcttca agataccgcc ggttaaaaag tccagtcagt ggatcacgga
      541 tggcctgttc tcggagctgg taatggagtg attcaatttg agcaattttg agattcagtt
      601 cttcgttggc cgtcttgagc gccagttcag caatcttgcg ggatgtaata tcttgtttaa
      661 tggcaataaa atgagtcact tcgccttttt cactgatgac aggagcaata tgttgctctt
      721 cataatagag gctcccgtct ttgcgtttgt tggtaatcga accaaaccac gttttgccgg
      781 ccagaaccgt gtcccaaatg tctttataaa atgacggctc gtgctggccg gatttgagga
      841 ttcgagtgtg atgaccgacc agttccttca aactataccc ggtcatccgc gaaaccgaag
      901 gatttgccca gcaaataact ccatgacagt cagtaatgat aatgccgttg gcggcagcgt
      961 tgagggcaac gctctgaaca tactgttcct gataggctga ccatcgaggc aacgcattgc
     1021 ggatggccgc taaaaccatt ccgacccggc ctccgatttc aggcagtggg ctgagcgcaa
     1081 tatcaaccgg gatttcaacc ccggatttat gccaggctga caaaattggc cgatttccca
     1141 taatgtgggg ttccggcgac cgatggtagt ccgctcgtaa ccgggcatgc tcggtgaact
     1201 gtggcggcac caggcattca acattttttc caatcagttc ttccggagca tatcccaaca
     1261 attcatatga tcggtcattt acaaacacca tatttccttg ccgatcaaca ataacgatga
     1321 catcgggact gccctggcaa atcgcttcat aaaattgttc tggagaatag gattcctgga
     1381 ataatgaatt cagaaccctc tcctttgaaa cggagtctaa attttgggta ttcaaaatga
     1441 gattctccca aagtggaatc aatcgcaacc agtgatgggc tatttttagg ggtaaggcaa
     1501 gcaagaagaa tgaggacgat gattgagtaa agatgttata gcagaactac ttatctgtcg
     1561 aaaagtattc tatggctgaa acctgggaaa ttgagaattg cctttggaaa ttcagggtgg
     1621 gtcaccaatt ttagggatat tcaactggaa tgaacccggc tgccaacccg gctttttcca
     1681 aatctgtttg cacctgaacc aatttcagct tagggtgcag cttcaaagtg acaatctcaa
     1741 tctgttgccc atattgccgg caggatttcg gtgaaccaca gcaaatcttt cggcacgaca
     1801 cccaactctt tgtaaggaag ttccaccggc atgactgagc aatcccacga ccatcacccc
     1861 tacactccgg ctgaagaact cgccaactgg ctttcccacg gtgccggatt tgtcggcagt
     1921 ctgatcgccc ttccaatttt ggtcgcttcg gcgttgggac aaagcaatct gcaaattgtg
     1981 gcggtgagcg ttttcgccgc gagcatgatg ctgctgtatc tggcttcgac gatttatcac
     2041 gttttaccac caagcaaaca taagcatctg tttcgaattg tggaccatgc tgcgatttat
     2101 gtgctgattg ccggaaccta cacgccgttc acgcttgggg tcttgcgtgg cgcctggggc
     2161 tggaccctct ttggcatcgt ctggagcatg gcgattgctg gaattgtctt taaagcattg
     2221 ggcggcatcc ggtttgacaa gctttcaacg gcgttgtatt tggcgatggg ctggctggtg
     2281 gtgattgcca tcaagccctt gattaccttg gtgccgatgg ctggactggt ctggctgttc
     2341 gtcggtggta ttttttatac cggcggcgtg gcgttttatg ccgcaaccag attgaaatac
     2401 caccacttcc tctggcattt gtgcgtgctg gccgggacat tttgtcattt cttcgcggtg
     2461 ttgtggtatt cgcagggctg aaaaaaccag ggctgaagac ttcgggcttg gggctgaggg
     2521 ctaggggctg aagaagtcgg gttgaagaac tggttttatt tcatccctca tccttcatcc
     2581 ctcatccctt caatcgcccc gagcttgcga gtcttcagcc ccaagccctg agccctggtt
     2641 ttttcagccc cgagcttgcg agtcttcagc cccaagccct gagccctggt tttttcagcc
     2701 c
//
LOCUS       DAOQDO010000701         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31827_length_2700_cov_2.753119, whole genome shotgun sequence.
ACCESSION   DAOQDO010000701 DAOQDO010000000
VERSION     DAOQDO010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31827_length_2700_cov_2.753119"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..>2700)
                     /locus_tag="PKZ53_11740"
     CDS             complement(405..>2700)
                     /locus_tag="PKZ53_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41154.1"
                     /translation="DMYTLRSDWKQAIRYQTEAISILEKVFGEKHPQTILFHLNLGEM
                     LVQTHDYEAAVLYYRKAITNAESTLGPTHPYYLIGLAGLAGFQLKTSQLSQAEATLTK
                     ALPLIEKVFGPTHPRLMMPLFFLAQLNQRQRKYQEAEAALLRAISIQEKNTNVRFTEP
                     GMYLSALSYIYQMSGKIEPALAYEMKSNDVVEQDFLKNISLGSERQKVLYLNRTKWGA
                     NHLLALQTQYAPHNQAAINAAVTSVLRRKGRALDVMADTLRLIQARATAQDQAIFEKL
                     IDAQNRLSNLTVKGPGTTKPERFQALVQELETEVDQLEAEISRQSAEYRVQTQPVTLE
                     NKQAAIPENTALIEFTLYQPLQNAQSSLNTKPRYLAYIIEPKGDPKWVDLGEVEKIDA
                     TTKEFRGVLQQSPGTRNRLIKAESRNAKKSNSGSVVLPDQQLRVLAQSLHQTVIKPVL
                     KMVKPGTALLLAPEGELNLVPFAALMDEDGTYLIEKQPLHYLTSGRDLLRLQVKTNSH
                     EPPLVVADPDYAVGSGPQVAGIQLAQLKRLIETAQEGVQIAKILPGSRLKVRGEATEQ
                     TLKQVNGPQILHVATHGYFLDRSSDVTLSADPIEEAEKRDIQIKGSGTKLDLIQFQSG
                     IFLAGANRGESGTEDGTLTALEAAHLNLSGTKLVVLSACNTGVGQVKTGEGVYGLRRA
                     MVLAGSETQMLSLWPVSDLGTRKLMVDYYTRLKAGEGRAEALRQVQLEMLKSRRFRHP
                     YYWASFVVSGEWSSPGSIGTLGVY"
BASE COUNT          628 a          746 c          644 g          682 t
ORIGIN      
        1 cccaaataac ctaccaggca gcagaaggca aaggaaattt tgccaccaat ctctcagacc
       61 tggctcaggc cgggtacatt gacaactcaa ttgcccaggc tcaatccact cccaaaaacg
      121 gctatcggct gagcgagata ataacgacac cagctacgat gaaagccgca gcaacctatt
      181 ggcttaccag ttccccgatt gtcacagaag gagcgacacg gactggaaat gattgtctgt
      241 atctcgatga aacggggatt attcgtcatt cgaaaagccc caaaatcaag cctggtgcca
      301 caagtgaacc aatctcgaaa ctggaaccga cctcgcttcc atctcatctc atccgagaaa
      361 aagaaacgat ccaatctgct cactcacaac tccacgctgg cggctcaata cacgcccaaa
      421 gtgccaatag aacctggact cgaccattcg ccggagacca caaaactggc ccagtaatac
      481 ggatgccgaa accgccggct cttgagcatt tcaagctgaa cctggcggag tgcttcggcc
      541 cggccctccc cggctttgag tcgggtgtaa taatccacca tcagttttcg ggttcccaga
      601 tccgacactg gccacagact cagcatttgc gtttcgctcc cggcgagtac catcgcccgg
      661 cgcagaccat agacaccttc gccggttttg acctggccaa ccccggtatt gcaggcggag
      721 agcaccacca gcttggtgcc gcttaaatta aggtgggcag cttccagcgc ggttaaggta
      781 ccgtcttcgg ttccgctctc ccctcgattg gcgcctgcca ggaaaatccc tgactggaac
      841 tgtatcaaat caagttttgt accactccct ttgatttgaa tatcccgttt ttcagcttcc
      901 tcaatcggat cagcggatag agtcacgtcg gaagaccgat ccagaaaata cccgtgcgtc
      961 gcaacatgga gaatctgtgg cccgttcacc tgtttgaggg tctgttcagt ggcctcgccc
     1021 cggactttca atctggaacc tgggagtatt tttgcaattt ggacaccttc ctgagcggtt
     1081 tcaatcaggc gttttaattg ggccagttgg attccagcca cctgtggacc actcccgact
     1141 gcataatccg gatcagccac caccagtggt ggctcgtggc tgtttgtttt cacctggagc
     1201 cgcaataaat cacgaccact cgtcaaataa tggagcggct gtttttcaat caggtaggtt
     1261 ccatcctcgt ccatcagcgc agcaaacggc accagattga gttccccttc tggagcaagc
     1321 aagagcgccg taccgggctt caccattttt aaaactggtt tgatgacagt ctggtgcaaa
     1381 ctctgcgcca gcaccctgag ttgctggtct ggcagaacga ctgatccact gttcgacttt
     1441 ttggcatttc gggattcagc cttgatcaac cggtttcggg tgccaggtga ctgttggagc
     1501 accccccgaa attccttcgt ggtggcatca atcttttcga cttcccccag gtcaacccat
     1561 ttcggatcac ctttcggttc aatgatatag gccagatacc ggggttttgt gttcagtgaa
     1621 ctttgggcgt tttgcagggg ctgatacagg gtaaattcaa tcagtgccgt attttcagga
     1681 atggcggcct gtttattttc cagcgtcaca ggttgggttt gaactcgata ttcggcgctt
     1741 tgccgactga tttcagcctc aagttgatca acctcggttt ccaattcctg aaccagtgcc
     1801 tgaaagcgct caggtttggt cgttccaggc cctttcaccg tcaggtttga aagccggttt
     1861 tgggcgtcaa tcaatttttc aaatatcgcc tgatcctggg ccgtagcccg tgcctgaatc
     1921 agcctgagcg tatcagccat cacatccaga gccctgccct tgcgtctcaa gactgaagtc
     1981 accgccgcgt tgatcgctgc ctgattgtgc ggggcatact gagtttgcag cgcaagcagg
     2041 tgattcgccc cccatttggt ccgattcaaa tacaatactt tttgccgctc agaaccaaga
     2101 ctgatgtttt tgagaaaatc ctgctcgacc acgtcattac ttttcatttc ataggccagt
     2161 gccggttcaa tcttgccgct catctggtaa atataggaca gagcactgag atacattcct
     2221 ggttcagtaa atcgaacatt tgtatttttc tcctgaatcg aaatagcccg cagcaaggcc
     2281 gcttcagctt cctggtattt ccgttgtctc tggttgagct gagccaggaa aaaaagcggc
     2341 atcatcaatc gcgggtgggt gggcccaaat actttctcta tcaaggggag cgctttcgtc
     2401 aacgtggcct cagcctggga tagctgactg gttttgagtt ggaaaccggc cagcccggct
     2461 agcccaatca gataataagg atgtgtcggt cccagagtgc tctcagcatt ggtgattgcc
     2521 tttcggtaat acaaaactgc agcctcatag tcgtgagttt ggactaacat ttcccccaaa
     2581 ttgagatgaa ataaaatggt ttgtgggtgt ttttcgccaa aaaccttctc caggatcgaa
     2641 atggcctctg tctgatagcg aatggcctgc ttccaatccg accgcaacgt atacatatca
//
LOCUS       DAOQDO010000702         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31878_length_2698_cov_2.338630, whole genome shotgun sequence.
ACCESSION   DAOQDO010000702 DAOQDO010000000
VERSION     DAOQDO010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31878_length_2698_cov_2.338630"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..514)
                     /locus_tag="PKZ53_11745"
     CDS             complement(38..514)
                     /locus_tag="PKZ53_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41155.1"
                     /translation="MKLFRIAMVAFVLCLTLSLQVSAKIWEHPAGFSIFIPDTYSVDI
                     DGDVLVAKDPTQELGIRLYAVENQESMEKAVDEAFELIKQNVTDLDLGEGQESKLNGL
                     DTLSFDGKGKVGGTPADLTVTLFHKNGKILVMYGAVNAAKASQFEADIVKILKSIK"
     gene            complement(626..1330)
                     /locus_tag="PKZ53_11750"
     CDS             complement(626..1330)
                     /locus_tag="PKZ53_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-tRNA-protein transferase"
                     /protein_id="HNJ41156.1"
                     /translation="MKTHSLSIMSLDGQYFRASHVEPEHMDLLWANGWRHFGSFFFRY
                     STTVWNGQVCTVLPLRIDLERFCLSKSQKRVLARNRDLEVVIRDTVIDDEKEELFFRH
                     VVRFDHNIPNSIFDFLSTQPATIPCQNQEICVYGTDGALLAASFLDLGQQATSAVYAM
                     FDPEETRRSLGIFTMLIAIRYSIERGCQFYYPGYAYREPSFYDYKKRFSALNYFDWWG
                     DWIPYQAEETVTSEFS"
     gene            1527..2639
                     /locus_tag="PKZ53_11755"
     CDS             1527..2639
                     /locus_tag="PKZ53_11755"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNJ41157.1"
                     /translation="MQKKLRVGVIFGGRSGEHEISLRSAESVLNALDKDKYDVIPLGI
                     TKAGKWLMAGDSQQLLPKEVVTEGEVSLAMMGDPTQSPLVRTNHNQIEGDVPTLDVVF
                     PVLHGTYGEDGTIQGLFEMANLPYVGCGVLASAAGMDKVIMKHLFRQAGLPIVDFTWF
                     TRLAWEKNPEPILNSVIESIGFPAFVKPANLGSSVGISKAKDRESLAKAITLAARFDR
                     KVVVEKGIDAREIELSVLGNDEPVASLPGEIVAGAEFYDYQDKYLSNTAELVIPAELT
                     EAQVADFQQMAIRAFQAIDGAGLARADFFLERSTGNIYVNELNTLPGFTSISMYPKLW
                     EASGLTYPQLLDRLIELAIDRHREKSRSVTSYDDQE"
BASE COUNT          695 a          626 c          703 g          674 t
ORIGIN      
        1 cttcaacccg acgtcttcag cccacgtatt ttcagtccta cttgattgat ttgaggattt
       61 tcacaatatc ggcttcgaac tggctggctt tggcagcgtt gaccgcgcca tacatcacca
      121 ggatttttcc gtttttatga aagagcgtaa cggtgagatc tgctggtgtc ccgccaactt
      181 tccctttgcc atcaaaggaa agcgtgtcta agccattcag cttgctttct tgaccttcac
      241 caagatctaa atcagtgaca ttttgcttga tgagttcaaa cgcttcgtca acagcttttt
      301 ccattgattc ttgattttca acggcataga ggcggatgcc aagttcttga gtggggtctt
      361 tggcaactaa aacatcaccg tcaatgtcaa ccgagtaggt atcaggaatg aaaatactga
      421 atccagccgg gtgttcccaa atcttcgccg aaacctgaag actcagagtc aggcacaaga
      481 caaaagcgac cattgcaata cgaaacagtt tcataaaaat tgttcctctt tcaaatgtgt
      541 gagcacgtga atgcagtgta agttgtgaat cagcggcaag atgtgagacg tgagaaatta
      601 gttggtcaga gcagactgat tctgctcagg agaactcgga ggtgacggtt tcttcagcct
      661 gatagggaat ccagtcaccc caccagtcaa aataattgag tgccgaaaac cgctttttat
      721 agtcatagaa ggacggctcc cggtacgcat agcctgggta gtaaaactgg cagccccgtt
      781 ctatcgagta ccgaatcgca atgagcatgg tgaaaatacc gagactgcgt ctggtttctt
      841 ctggatcaaa catggcatag accgctgagg ttgcctgctg ccccaggtcg agaaagctgg
      901 ctgccaggag cgccccatct gttccataaa cacagatttc ctggttttga caggggatgg
      961 tggccggctg ggttgagaga aagtcaaaga tggagttggg aatattgtga tcaaatcgaa
     1021 cgacgtgacg aaaaaagagc tcttcttttt catcatcaat gacggtatct cggatcacaa
     1081 cctcaagatc acgattgcgg gccagcaccc gtttttgact tttcgagagg caaaagcgct
     1141 ccaggtcaat gcgcagaggc aagacggtgc atacctgacc attccagaca gtggtggagt
     1201 agcggaaaaa gaagcttcca aaatgtctcc agccatttgc ccagagcagg tccatgtgtt
     1261 caggttcgac gtgcgaagca cgaaaatatt gtccatcaag cgacataatt gagagtgagt
     1321 gagtcttcaa acacactgtc ggaattcaaa aagtcggggt tttcattctg aagcacaatg
     1381 ccggaaatga aaatcaaaat ttttggcatc cagacaggaa agttcaaccg gcagcaaggt
     1441 ctggaacact cacccttgtg ttcaagctca aagaaaaata ttgtttcggt ttccattttt
     1501 taactgattt gtttctggag gcacctgtgc agaaaaaact acgggtcggg gtcattttcg
     1561 gtggacgctc cggcgagcat gaaatctcgt tgcggtcggc ggaatccgtc ctcaatgccc
     1621 tcgacaaaga caaatatgac gtgattccgc ttgggatcac caaagccgga aaatggctta
     1681 tggctggcga ttcgcaacag ttgttgccca aagaggtggt cactgaaggc gaagtcagtc
     1741 tggccatgat gggtgacccg acgcagtcac cgctggttcg aaccaaccac aaccagattg
     1801 aaggcgatgt cccaaccctg gatgtggtgt tcccggtgtt gcacggcaca tatggcgaag
     1861 atggcacaat tcaggggctg tttgaaatgg ccaatctgcc ctatgtcgga tgcggtgtac
     1921 tggcttcagc cgccgggatg gacaaagtca tcatgaagca cctgtttcgt caggctggtc
     1981 ttccaattgt tgattttacg tggtttacgc gtctggcctg ggaaaagaac ccggaaccta
     2041 tcctcaattc cgtgattgaa tccattgggt tcccagcctt tgtgaagccc gcaaacctgg
     2101 gatcatccgt tgggattagc aaagccaaag atcgtgaaag tctggccaaa gccattaccc
     2161 tggcagctcg atttgaccgc aaagtggtgg ttgaaaaggg aattgatgcg cgtgaaatcg
     2221 aactcagcgt gcttggcaat gatgaaccag ttgccagttt gccgggtgag attgtcgccg
     2281 gagcagaatt ttatgattac caggataaat atctcagcaa cacggctgaa ctggtgatcc
     2341 cggctgaact gaccgaagcc caggtggctg actttcagca aatggccatc cgggcatttc
     2401 aagcgattga cggggctggg ctggcacggg ccgatttctt cctggaacgt tcaactggaa
     2461 acatctatgt caatgaactc aataccttgc cggggttcac ttcgatcagc atgtatccaa
     2521 aattgtggga agccagtggg ttgacctatc cacaactttt ggaccggttg attgaactgg
     2581 cgattgatcg tcatcgcgaa aaatcgcgct cagtcaccag ttatgacgat caggaatagt
     2641 gcctggtgcc tggtgcttgg tgcctggtgc ttggtgcttg gtgcttggtg cttggtgc
//
LOCUS       DAOQDO010000703         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31879_length_2698_cov_2.328415, whole genome shotgun sequence.
ACCESSION   DAOQDO010000703 DAOQDO010000000
VERSION     DAOQDO010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31879_length_2698_cov_2.328415"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..516
                     /locus_tag="PKZ53_11760"
     CDS             118..516
                     /locus_tag="PKZ53_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41158.1"
                     /translation="MSTGKGEGDYGSLTKAQFDALIQAGTRVVFSGHEHNFEYAKAAN
                     YNLHCIISGAGGALRDNQLSHDSLQKASIQSWSNQAHFLIVTIKAPDRGVMEIRVIGA
                     NANGTPNEAVPVWKTASSPVPEPMPLKIRL"
     gene            complement(691..1011)
                     /locus_tag="PKZ53_11765"
     CDS             complement(691..1011)
                     /locus_tag="PKZ53_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41159.1"
                     /translation="MPFFARLIVFCVLVGLAFIILPIQYRYIQGYITMLGNPLRLLVG
                     HLFAVIGMVGAVGVSAYWMVASLRQNQKSKDQTPAPRSLHPTESRVDQPSLNLQRGRS
                     ERQK"
     gene            complement(1031..2557)
                     /locus_tag="PKZ53_11770"
     CDS             complement(1031..2557)
                     /locus_tag="PKZ53_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ41160.1"
                     /translation="MKEFIRGQKSKLSDLTPAQTVDVDIQLTFPGSRVVDISCFGVDA
                     SNQLSDDRYFIFYNQKQSPEGALTLAGNLGGDRERFTVDVSRLPQTIRKLVFTATLDG
                     DGTMAELSQGQFQIVAQGQPIVMFRFAGSDFGTEKAIIVAEIYFKDVWRFGTVGQGFS
                     GGLSALLKHFGGSETSQPAPPLPSIQAPPPAPSFAPPPPPISPPPYNPPAPTAPFQSP
                     AATTPFYSPPSYPPPPAPVTQPLGPPAGPPSYPPPPVMSPYPAPPPPAYPPPPAYPPP
                     AYPAPPIPPAYPPPPPMMGIPPMAPPQPPPPAGPTKVTLDKKGDRKAVSLKKEAGARP
                     IHINLNWDNPNANKGFFGFGKAAAAPDLDLGCMFELINGDKGVIQPLGGYFGARNEPP
                     FIFLDKDDRSGAAADGENLYIYRPDLINRVMIFGLLYEGADDFRSVNGRVTIRDQHGN
                     EIFMWLNNPEPNQILCAACLIQRTPNGLEIIKEERYFQRASQADQHYQFGFQWSRGSK
                     "
BASE COUNT          644 a          657 c          715 g          682 t
ORIGIN      
        1 ccttgttcat aagttccttc tgtatttaaa gatgtaacgc cccagggttt aaaaggaatc
       61 ccatttttcc atcacccacc gttttgtgcc gggccaaccc atgcctctcg tctcaatgtg
      121 tcaactggaa aaggtgaagg cgactatgga tccctgacca aggcacaatt cgatgcgttg
      181 attcaggcag gaactcgggt tgtttttagt ggtcacgagc acaactttga atatgcaaaa
      241 gcggcaaact acaacttaca ttgcattatt tcaggtgctg gcggcgctct gcgagataat
      301 cagctctctc acgattcatt gcagaaagcc tcgatccaaa gctggtcaaa tcaggctcac
      361 tttctgatcg ttaccattaa agctcctgat cggggagtta tggaaatcag ggtgattggc
      421 gccaatgcca acggtacccc taacgaagca gttccagtct ggaaaacagc cagttcgcca
      481 gttcctgagc ctatgccatt gaaaatcagg ctctgactgc tcggaaccag acggtgttat
      541 agatcttcag aaaaaggttc agagtatcgc cttcaggcca gagggttttc tctgtcgtcc
      601 cactcgtcaa acctcacgcc tgaaggcgtt actctgaaca gaaatcttta tctgaaaggc
      661 tatagatatg gattgaagaa tggaaaagcg tcatttttgt cgctcagatc tcccgcgttg
      721 aagattcaag gaaggttgat caacacggga ttcagtcggg tgtaaagacc tgggtgcagg
      781 tgtttgatct ttagactttt gattttgtct caggctggca accatccagt atgcagagac
      841 tccgactgcc cctaccatac ctatcacagc aaataaatgt ccgaccagga gccgcaatgg
      901 atttccaagc attgtgatat agccttgaat gtagcggtac tgaattggca aaataataaa
      961 agccaacccc accagaacac aaaacacaat caggcgagca aaaaaaggca tataagttcc
     1021 tacgctggcg ctatttagat ccccgcgacc attggaatcc aaactgataa tgctgatcgg
     1081 cctgtgaggc gcgctggaag taccgctctt ccttaatgat ttcgagcccg tttggtgtac
     1141 gctgaattaa acaggccgca cagagtattt gattgggctc tggattattg agccacataa
     1201 agatttcatt tccgtgctga tcccggatcg tcacgcgacc gttgacggat cgaaaatcat
     1261 ccgcaccttc atacaggagc ccaaaaatca tcactcgatt gattaaatcc gggcgataaa
     1321 tataaagatt ctctccgtca gccgcagctc ccgaacggtc atctttatcc aggaaaataa
     1381 aaggcggctc attgcgcgcc ccaaagtacc ctccgagcgg ttgaatgacc cctttgtctc
     1441 cattgatcag ttcaaacata cacccgagat ccaggtccgg tgcggctgcg gcttttccga
     1501 atccaaaaaa tcccttgttg gcattggggt tatcccagtt gagattgata tggattggcc
     1561 gcgcaccggc ttctttcttt aaactcacgg ctttgcggtc gccttttttg tccaacgtaa
     1621 ctttggttgg accagccgga ggtggaggct gcggcggcgc catcggtgga attcccatca
     1681 ttggcggtgg cggtgggtaa gctggcggaa tgggtggggc cggataggct ggaggtggat
     1741 aggctggcgg aggaggataa gccggaggtg gtggcgccgg atacggtgac atcactggcg
     1801 gtggtggata agacggaggc cctgccggtg gacctaacgg ctgagtcacc ggcgctggtg
     1861 gcggcggata cgaaggcggt gaataaaatg gcgtggttgc cgcaggtgat tgaaatgggg
     1921 cggtcggggc tggtggattg tatggtggtg gactgatcgg cggtggtggt ggtgcaaacg
     1981 agggtgcagg aggtggcgct tgaatcgaag gcagcggcgg tgctggttgt gatgtttcgc
     2041 tgccgccaaa atgcttgagt aacgcgctca gtccaccgct aaacccctgg ccgaccgtcc
     2101 caaatcgcca gacgtccttg aaataaattt cagcgacaat gatggccttc tcggtaccga
     2161 aatctgaccc ggcaaaccga aacatcacaa tgggctggcc ctgcgcaaca atctgaaact
     2221 ggccttgtga cagctcagcc attgttccat cgccgtcaag cgtcgcggta aacacaagtt
     2281 ttcgaatggt ttgagggagt cgtgagacat ccacggtgaa ccgttcgcga tcaccgccaa
     2341 ggttcccagc caacgtcaaa gcaccttccg gcgactgctt ctggttatag aaaatgaaat
     2401 accggtcgtc tgaaagctga ttgctggcat ccacaccaaa acaactgatg tccacgactc
     2461 tcgaacctgg aaaagtcagt tgaatgtcaa cgtccacggt ttgagcaggc gtcaggtccg
     2521 acaattttga tttctgccca cggataaact ctttcatagt caccgccttt ttttgaaaga
     2581 ttctatcggt tcctggtttt tggtttcctg gtgcttggtt cttggttcct ggtttttggt
     2641 ttcctggtgc ttggttcctg gttcttggtt cttggttctt ggttcttggt tcttggtt
//
LOCUS       DAOQDO010000704         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31948_length_2695_cov_2.586364, whole genome shotgun sequence.
ACCESSION   DAOQDO010000704 DAOQDO010000000
VERSION     DAOQDO010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31948_length_2695_cov_2.586364"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            596..2641
                     /locus_tag="PKZ53_11775"
     CDS             596..2641
                     /locus_tag="PKZ53_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ41161.1"
                     /translation="MPPKVFQTLLILVQNNQKLVTRDEIINYIWPNTFVEDHNLNANI
                     STLRRILNECVPGVKFIETVPKRGYRFVADVQMVPSTTSRQEINDASTISTLSPMQKA
                     AELSIEAGESFGPDSDLPDSFPPLISDPPVELPVVEFQTVEPEADLNAEEPLQVETKP
                     EPITEKAPRPVVLRMPLSEKPAVPHPVRAFQNLKFVAILSLVVFLGLISTPSTLLTSK
                     ASFLAELQATNIAVLPFKVIGSGDPALGVGAADTLITQLGQVPGFTILSGQAVENVLE
                     NRELSPTEAARLLRVQAVIEGSLQLLDGRLRATVRMISPRSSSPVWTEVIDFTGENKA
                     SHQDQIARRVARILLDATSPSTHPTGTSNETAYRYVLNGRYHWSRRDSQSLAESEACY
                     RRALQLDSQYAEAYLGLADNLIFEEVGSERALEAWEHLQQALKLNPKLGEAYATLGFI
                     QGFHQRNWNEAEVAFRRALELSPNSTKCHHWYAYHLAVTGRINEAIREMKITLALEPT
                     SPNFLADLGQMCYFERDFEGGRRYCRRAWQIDPYFYYVYSNLNPILAKQGNYAKAMRA
                     YELEKSSFYERVHNTEDVFASDSGDPADITRFREALRKEAEMILARPDHESLSYKLST
                     IYAQLGETEQSCFWLAKAIAKKQLLTPFANIDPIFDPLRDQPEFSTLLAQMNLGKGN"
BASE COUNT          695 a          686 c          629 g          685 t
ORIGIN      
        1 taggaggctt ttttccgcca ctcgtcctcg gaaatatcaa acagttgacc ggatcattta
       61 ccggcggatt tatcctgctg gcaatctttg cctttgtctg cctgatcgtg gtttggaaaa
      121 ctgattcgca gggtgattcc aaggtaacgc aaccagcctg aagaacgctc gcgaattgct
      181 agaaaagaaa acgcatcctg gagctgtcgg taatctgaca ctccaggatg cgttttcttt
      241 tctacggcaa atgaagactg gtgacgtgac ggctgactga aaaaaagaaa atttcgtgat
      301 gttgggagac atctttcctg gctgatttag ccgtatcagc caagttattc aatccggtta
      361 cttcttgcta accttacccg ttcaggaaaa acatctccaa catcgttgaa ggccattttg
      421 gatattttct cggaaatatc ctcaataaaa catcattttt atctcaaaat tcttgtggag
      481 aagctcgatg ttaagctctg aggttcataa tcaagaaaca gctttgtttc tctttggcaa
      541 ctttcgaatt gatctttcac aacaatccct tgcatacaat aatgaattta taccgttgcc
      601 tccaaaagtg ttccagactt tattgatttt ggttcaaaac aaccagaagc tggtcacaag
      661 ggatgaaatc atcaattaca tctggccaaa cacctttgtt gaagaccaca acctgaatgc
      721 caatatttct accttgcggc gaatcttaaa tgagtgtgtc ccaggggtaa aatttattga
      781 aacggttcca aaacgcgggt accggtttgt ggccgatgtc cagatggtcc catcaacgac
      841 ctcacgccag gaaatcaacg atgcctccac catttcaacg ttgtcgccga tgcaaaaggc
      901 agcggaactt tccatcgaag cgggtgaatc ctttgggcca gacagtgacc tgcccgactc
      961 gtttccgcca ctgatctctg acccaccagt ggaattgcca gttgtcgaat ttcagacagt
     1021 tgaaccagaa gctgacctca acgctgagga accactccag gtggaaacca aacctgagcc
     1081 gatcacagaa aaagcgccca ggccagtggt tctcagaatg ccactttcag agaaaccagc
     1141 cgtccctcat ccggttcgag cctttcagaa tctcaaattt gtggccattc tcagtcttgt
     1201 cgttttcctg ggcctgattt caaccccatc cacgcttttg acatcaaaag ccagtttttt
     1261 ggctgaactc caggccacca acatcgcggt tcttccattt aaagtcattg ggagcggcga
     1321 tccagcactg ggggttggcg ccgcagatac cctcattact cagttgggtc aggtgcctgg
     1381 gtttaccatc ctttcaggcc aggcagttga aaatgttctt gaaaaccgtg agttgtcccc
     1441 aactgaggct gcccgcttgc tgcgagttca agccgtcatc gaaggcagtt tgcaactcct
     1501 tgatggacgt ctccgggcta ccgtgcggat gatttctccc cgctcgtcgt cgccagtctg
     1561 gacggaagtt attgatttca caggtgaaaa caaagcttcg catcaagatc aaattgccag
     1621 gcgggtggcc agaattctgt tagatgccac cagtccttcg acccacccga cgggaacctc
     1681 caatgaaaca gcctatcggt atgtgctcaa cggacgctat cactggtctc gtcgagattc
     1741 gcaatccctg gctgaaagcg aagcctgtta tcggcgggcg ctccaacttg actcacagta
     1801 tgctgaagcc tacctcggac tggcggataa cttgatcttt gaggaagtcg ggtccgaacg
     1861 agcattggaa gcctgggaac atctgcaaca ggcactcaag ctcaacccaa agctgggtga
     1921 agcctatgcc acgcttggtt ttattcaggg gtttcatcag cggaactgga atgaagctga
     1981 agttgccttc cgacgcgccc tggaactgtc accaaactca accaaatgtc accactggta
     2041 tgcctaccat ctggccgtga caggaaggat caacgaagca atccgggaaa tgaaaatcac
     2101 gctcgcactg gagccaacct caccaaactt tttggctgac ctggggcaga tgtgctactt
     2161 cgagcgggat tttgaaggtg gtcggcgcta ttgtcggcga gcgtggcaga ttgatcctta
     2221 tttttattat gtctattcca acctcaatcc aatcctggcg aaacaaggca attacgccaa
     2281 agccatgcgg gcttatgaat tggaaaaatc ttcgttctat gagcgggttc acaacaccga
     2341 ggatgttttt gccagtgatt caggtgaccc ggcggatatt actcgattcc gagaggcact
     2401 ccgcaaagag gccgagatga ttctggctcg cccagaccac gagtcattga gctataaact
     2461 gtcaaccatc tatgcccagc tcggagaaac tgaacagtcc tgtttctggt tggccaaagc
     2521 gattgcaaaa aagcaattgt tgaccccgtt tgccaacatt gatccgattt ttgatccact
     2581 gcgggatcaa cctgaatttt ccacgcttct ggcccagatg aatcttggca aaggaaacta
     2641 ataccaattt gggctgaggg tatcgggcta agggctgaag aaaatacaat atttt
//
LOCUS       DAOQDO010000705         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_31957_length_2694_cov_4.059871, whole genome shotgun sequence.
ACCESSION   DAOQDO010000705 DAOQDO010000000
VERSION     DAOQDO010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31957_length_2694_cov_4.059871"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..1069)
                     /locus_tag="PKZ53_11780"
     CDS             complement(314..1069)
                     /locus_tag="PKZ53_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNJ41162.1"
                     /translation="MKLTLYVWRQKGPNEKGQMVKYEAPNVNEHMSFLEMLDVVNEDL
                     IAKGEDPIAFDHDCREGICGMCGVVINGMPHGGHRGTTTCQLHMRHFRDGESVYIEPW
                     RSKGFPVLRDLVVDRSAFDRIIGAGGYVSVPTGAVPDANAILVSKPDADRAMDAAACI
                     GCGACVAACPNGSAALFTSAKIGHLGVLPQGQPERYKRVLAMVDQMDSEGFGHCTSHR
                     ECEAACPKEISFEFITRMNRDYLKASLTDKNGG"
     gene            complement(1071..>2694)
                     /locus_tag="PKZ53_11785"
     CDS             complement(1071..>2694)
                     /locus_tag="PKZ53_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNJ41163.1"
                     /translation="YDTVKGGDFRAREANVYRLAQVSVNIIDQCVAQGVPFAREYGGL
                     LDNRSFGGAQVSRTFYARGQTGQQLLIGAYQALERQIHLGNVKMFSRTEMLDLVVVNG
                     VARGIVTRDLVTGKIDSHVGDAVILGTGGYGNVFFLSTNAKGSNVTASWRAHKKGAAF
                     ANPCFTQIHPTCIPVSGDYQSKLTLMSESLRNDGRIWVPLKKGDSRSPDQIPEGERDY
                     YLERKYPSFGNLVPRDIASRAAKDVCDQGRGVGETGLGVYLDFGDAIKRVGEKVISEK
                     YGNLFDMYERITGENPYKVPMRIFPAVHYTMGGLWVDYNLMSTIPGLFVLGEANFSDH
                     GANRLGASALMQGLADGYFVIPYTIGNYLAQAKLDKLDKAHTDFQKTEADVKGRINKL
                     LNLKGKRTVDSFHRELGKIMWDYCGMARTEKGLTHALAKIPEIKEEFWKNVSVLGSGE
                     ELNQSLEKAGRVADFIELGELMCRDALNRNESCGGHFRVEYQTEEGEAMRNDEAYSHA
                     AAWEYTGENKAPILHKEPLVFEYVKPSQRSYK"
BASE COUNT          566 a          769 c          688 g          671 t
ORIGIN      
        1 gtcatccgtc atccttcatc ccttatccct tcgattaccc caagccctaa actgagctga
       61 agagatcggg cttggtgtga ctcaagggat aaattcttca gccctgagcc cagcccaacg
      121 tcttcagccc tgagcttgcg aatcttcagc cctcagtctt caggttacct gttatcgcgt
      181 ggttttcctg aaattcagct cagttggaaa agaaaaggac ctgctcacgc aggtccctgt
      241 tggtttcttt ctcactggtt gactgttccc gatttcttca gccccgagcc ctcagcccca
      301 agccctggta ttttcagccc ccgttcttat ccgtcaggct ggccttgagg taatcccgat
      361 tcatgcgggt aatgaattca aagctgattt cctttggaca ggccgcttca cattcacggt
      421 gactggtgca gtggccaaat ccttcgctat ccatctgatc aaccatggcc agaacgcgtt
      481 tgtaccgttc gggttggcct tgtggcaaga cacccaggtg accaattttg gccgaggtaa
      541 acaaggcagc cgagccattt ggacaggccg ccacacaggc accgcacccg atacaggccg
      601 ccgcatccat ggcccggtcc gcatctggtt ttgaaaccag gatcgcgttg gcatccggaa
      661 cggcgccagt tgggacagaa acatagccgc cagcgccaat aatccgatca aaggcgctac
      721 ggtcaaccac cagatcgcgc agaaccggaa agccttttga ccgccagggt tcgatataga
      781 ctgactcacc atcgcggaag tgccgcatgt gaagctgaca ggtggttgtt ccacgatgtc
      841 caccgtgcgg catgccattg atgaccacac cacacattcc acaaatcccc tcgcggcagt
      901 cgtggtcaaa tgcgattggg tcttcccctt tggcaatcag gtcttcgttg accacgtcaa
      961 gcatttccag aaaggacatg tgttcattga cgttgggtgc ttcgtatttc accatctggc
     1021 ccttttcgtt cgggcctttt tgtcgccaaa cgtaaagcgt cagtttcatg ttatttataa
     1081 ctccgttggc taggtttcac atattcaaag acgaggggtt ctttgtgcag gattggggcc
     1141 ttgttttcgc cggtatactc ccaggctgcg gcatgggaat acgcttcgtc attgcgcatt
     1201 gcttcccctt cctcagtctg gtattcaacc cggaagtggc cgccacacga ctcattgcgg
     1261 ttgagtgcat cacggcacat caattcgcca agctcgataa agtccgccac acggcctgct
     1321 ttttcaagcg actggttgag ttcttcaccg ctcccgagga cgctgacgtt tttccagaat
     1381 tcctctttga tttcaggaat tttggccagt gcatgcgtca agcccttttc agtgcgggcc
     1441 atcccacaat aatcccacat gattttgccg agttcgcggt ggaaggagtc aactgtgcgc
     1501 ttacccttga gattgagcag tttgttgatc cggcctttga catcggcttc tgttttttgg
     1561 aagtcggtgt gggccttatc cagtttgtcg agtttggcct gggccagata atttccaatg
     1621 gtgtacggaa tgacaaaata cccgtccgcc agtccctgca tcagggcgct ggcacccaat
     1681 cggttggcgc cgtggtctga gaagttggct tcacccagga caaacagacc tggaatcgta
     1741 ctcatcagat tgtaatcaac ccagaggccg cccatggtgt agtgcaccgc cgggaaaatc
     1801 cgcatcggga ctttgtatgg gttttcgccc gtaatccgct catacatatc aaacaggttg
     1861 ccatattttt cgctgatgac cttttcgcca acccgtttaa ttgcgtctcc aaagtcgaga
     1921 tagacgccaa ggccggtttc gccgacgccc cgtccttggt cgcacacatc tttggcggcg
     1981 cgtgaagcaa tgtcgcgcgg caccaggttt ccaaagcttg ggtatttgcg ttccaggtag
     2041 taatcgcgtt caccctctgg gatttgatca ggcgaccgtg aatcaccttt tttgagcggc
     2101 acccagatgc gaccgtcgtt gcgcaacgat tcgctcatca gggtcagttt tgattgatag
     2161 tcaccactga ccggaatgca ggtcgggtga atctgggtaa agcaaggatt ggcaaacgcg
     2221 gcgcctttct tgtgggcgcg ccagcttgcc gtcacgtttg agcctttggc gttggttgac
     2281 agaaaaaaga cgtttccata tccgccagtg cccaaaatga ccgcatcgcc tacgtgggag
     2341 tcaattttcc cggtgaccag atcgcgggtc acaatcccac gggccacgcc gttaaccacc
     2401 accagatcca gcatttctgt ccgggaaaac attttgacgt tccccaggtg aatctggcgc
     2461 tctaatgcct gataggcgcc aatgagcaac tgctgaccag tctgaccacg ggcatagaag
     2521 gttcgcgaga cctgggcgcc gccaaaggac cggttgtcga gcagtccgcc gtattcacgg
     2581 gcaaacggga caccctgggc cacgcactgg tcaatgatat tgacgctcac ctgggccagc
     2641 cgatagacgt tggcttcacg ggcgcgaaag tctccacctt ttactgtgtc gtaa
//
LOCUS       DAOQDO010000706         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32038_length_2691_cov_2.854704, whole genome shotgun sequence.
ACCESSION   DAOQDO010000706 DAOQDO010000000
VERSION     DAOQDO010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32038_length_2691_cov_2.854704"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKZ53_11790"
     CDS             complement(<1..1033)
                     /locus_tag="PKZ53_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41164.1"
                     /translation="MRSKIFLALWLGWLCAVTGCGRVGDPVAPPSRSMENTQLVEVIQ
                     RGDRVLVRWPRPNLKNPQTSGVSRVDIYRLIDEPKTQRFLREEIFLEQAQLVGYLTGE
                     QLAASDGAFIVFEDRLDPADQSWQNQRFRYAIRYTDTRNRQGALSNFAFVEPSATIAL
                     QPTGLTSTFNQSAISLEWKAPSQTLAGTAIGTAPRYNIYRRGPKGEFSRRALNPTPLT
                     EARFQDTQFQFENTYVYTVRTVTEVGGVLTESDDAEVTTVSPKDTFAPAAPQNVTGAA
                     AAGVTSLFWPSNSEADLKGYLVYRAESSDAPTWQRLTPNPISTTTFRDLSAEVGKVYL
                     YRLTAVDVFA"
     gene            complement(1227..2621)
                     /gene="hslU"
                     /locus_tag="PKZ53_11795"
     CDS             complement(1227..2621)
                     /gene="hslU"
                     /locus_tag="PKZ53_11795"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNJ41165.1"
                     /translation="MVIYLSGDIAEDQGPRLDDMTPRQIVVELDKHVVGQRAAKRAVA
                     VALRNRIRRQKLPPDLAQDVMPKNIIMIGSTGVGKTEIARRLARLANSPFLKVEASKF
                     TEVGYVGRDVEAIIRDLVEIGIELVRQEKTDEIQDKADLNAEERLLDILLPNPPRVED
                     SLEPTGEDPMTRTREKLRQQLREGKLDQRMVEIEVQEKNFPAGIEIITPQGIEEMDIN
                     LRDMIPGFFPGSKTKKRQMTVEEAMEYLIQEEEQKLVDMEQVTRIALERVENSGIVFL
                     DEIDKIAGRESGHGPDVSREGVQRDILPIVEGTTVSTRYGMVRTDHILFIAAGAFHVS
                     KPSDLIPELQGRFPIRVSLDPLTREDFKRILTEPKNSLIRQYTALMETEGVTLEFTPD
                     AIESVADFAFSVNQQTEDIGARRLHTVMEKLLDEISFNAPDLEEKHQVINSAYVTEML
                     AGIVQNEDLSRYIL"
BASE COUNT          605 a          699 c          704 g          683 t
ORIGIN      
        1 tggcaaacac atccactgcc gtaagccgat agaggtacac ctttccaact tcagccgaca
       61 ggtcacgaaa cgtcgtggtg gaaattggat ttggcgtcaa ccgctgccag gttggagcat
      121 cgctgctttc ggcccggtac accagatatc cttttaagtc cgcttcactg ttcgaaggcc
      181 aaaacaggct tgtcactcca gccgccgcgg cacccgtcac gttttgaggt gcggctggcg
      241 caaaggtgtc tttgggcgag accgtggtga cttcggcatc atcactttcc gtcaacacac
      301 ctccgacctc ggtcaccgtg cgaaccgtgt agacataggt gttttcaaat tgaaactggg
      361 tatcttgaaa acgggcttca gtcagtgggg tggggtttaa tgcccggcgg ctaaattcgc
      421 ctttcgggcc acgccgatag atgttgtagc gtggggcagt tccaatcgcg gttccggcca
      481 gtgtttgcga cggtgctttc cattcaaggc tgattgccga ctggttaaaa gtgctggtta
      541 acccggttgg ttgcagggca atcgtcgccg acggctcaac aaatgcaaaa tttgagagtg
      601 cgccctgccg gttacgggtg tcggtatacc ggatggcata gcggaagcgc tggttttgcc
      661 agctttgatc agccggatcc agccggtctt caaacacgat aaaggcaccg tctgaagccg
      721 ccagttgctc gccggtcagg tatcccacca gttgggcctg ctccaggaaa atttcttcgc
      781 gtaaaaatcg ctgggttttt ggttcgtcaa tcaatcggta gatgtcaacc cgtgaaactc
      841 ctgaagtttg cgggtttttg agattggggc gcggccagcg aaccagcaca cgatccccac
      901 gctggatgac ttcgaccagt tgcgtgtttt ccattgaccg cgaaggtggc gccaccgggt
      961 caccaacgcg accacagcct gttacggcac acaaccaccc cagccagagt gcaagaaaaa
     1021 tttttgagcg catgaagtac tgagaatacc agtttgtagt cagtagtcag tagtcagtag
     1081 ttcactaagt ctatttgatt gaattacttg actgttttcc gatgcgaggt tctattccaa
     1141 aatgggatac ggagcaaaga agcttcagtg ctgatgatca gcctgggttc agtcaaccct
     1201 taaatttctt gtctttcgct ccgcggctaa agaatatacc gacttaaatc ttcattctgg
     1261 acaatcccgg ccagcatctc ggtgacatag gcactgttga tcacctgatg tttttcttcg
     1321 aggtccgggg cgttaaatga aatctcatca agtaactttt ccatcaccgt atgcaaccgg
     1381 cgggcgccga tgtcttcggt ctgctggttg acggaaaagg caaaatccgc caccgattca
     1441 atcgcgtctg gtgtaaattc aagcgttacg ccttcggttt ccatcaacgc cgtgtactga
     1501 cgaatgagtg aatttttcgg ctcggtcaga atgcgtttaa agtcttcacg ggtcagtgga
     1561 tccaggctca cacggattgg aaatcggcct tgcagttctg gaatcagatc tgatggtttg
     1621 gaaacatgaa acgccccggc ggcaataaac aaaatgtgat cagtccgcac catgccatac
     1681 cgggtgctga cggtggtgcc ttccacaatc gggagaatat cgcgctgcac gccttcccgt
     1741 gagacatcgg ggccgtggcc ggattcacga ccggcaattt tgtcaatttc atcaaggaaa
     1801 acgatcccgg aattttcgac tcgttcgagc gcaatccggg tcacctgctc catgtccacc
     1861 agcttttgtt cttcttcctg aatcaagtat tccatggctt cctcaaccgt catttgacgc
     1921 tttttggttt ttgagccagg gaaaaaaccg ggaatcatat cgcggagatt gatgtccatt
     1981 tcctcgattc cctgtggcgt gatgatttca ataccggcgg gaaaattctt ctcttgaact
     2041 tcgatttcaa ccatgcgctg gtccagcttg ccctcgcgga gttgctggcg aagtttttca
     2101 cgcgtgcgtg tcattggatc ttcgccagtt ggttccagcg aatcttcaac ccgaggcggg
     2161 ttgggcagga gaatatcaag cagccgctct tcggcgttga gatcggcctt gtcctgaatc
     2221 tcgtcagttt tctcctggcg caccagttca atgccaattt caaccaggtc acggatgatg
     2281 gcctccacat cacgcccaac atagccgact tcagtaaatt tcgacgcttc gacttttaaa
     2341 aacggagagt tggccagacg tgccagacga cgggcaattt cagtttttcc gaccccagtt
     2401 gaaccaatca tgatgatgtt tttgggcatc acgtcctggg cgagatcagg cggcagtttt
     2461 tgacggcgaa tgcgattacg gagggcaacc gccaccgctc gcttggccgc ccgctgacca
     2521 acaacgtgct tgtcgagctc caccacaatt tggcggggtg tcatatcatc aagtcggggg
     2581 ccttgatctt cagcaatatc accagataaa taaataacca tagaatggat cgtcagcagt
     2641 aaaagtctct gggttaaatg atgaactgcg gttgtgcgtt tgggtcgcag g
//
LOCUS       DAOQDO010000707         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32056_length_2690_cov_3.488805, whole genome shotgun sequence.
ACCESSION   DAOQDO010000707 DAOQDO010000000
VERSION     DAOQDO010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32056_length_2690_cov_3.488805"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKZ53_11800"
     CDS             complement(<1..657)
                     /locus_tag="PKZ53_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HNJ41166.1"
                     /translation="MRRRGADVGSGFQEALNRTNEAYEKAGLACITRKAIPGKYTVSR
                     ENKRRGLSLPSVEGLNVLQSGARLSAGDLQSILKSEGARDPRQFVPESRAEPDYGGCI
                     APTGRAIYYDAKSTSREQLDFDNLHPHQVSYLERTANVGAVAGFLVEFSAHQKVFFIP
                     IQVLMKFKASTTRKSVPYRFFEANLIPVKPGKGLLIYDYLQTIADQEAQYGKDFSGLK
                     I"
     gene            complement(862..2220)
                     /locus_tag="PKZ53_11805"
     CDS             complement(862..2220)
                     /locus_tag="PKZ53_11805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41167.1"
                     /translation="MKECPTCNSCYDDSARFCIQDGSSLAESLPGSRTFHNRYQLIRM
                     IGRGRLGDVYQATDLTNQRQVSIKIIPPNLFTTREAETTFQQEVQRLINISSPYVAST
                     FGYGLLPNAASYLVSEYIEGRTLKDELEEEKILQTPRALEVIRQSAIGLGVAHDAKVL
                     HLDLKPGNLLLMADEVTRELQVKVVDFGLACLKDSLGGAVVTDTGSVIFRMPHYTSPE
                     ECTGQATDQRSDIYSLGIILYEMLAGRVPFSEKQPMKVILAHATKAAQPLRDLNSDVP
                     PPVEALVMKMLAKDPAYRPQSMAAVIAAIEQVQPSKKESVMYKGAVNPQAESSPPKVE
                     PDPSSLNLPLRLTIIDTDDEGNKSRTIPGVVHDASPQGMRIQTGTVATGHLNIVKDHT
                     VAFKNRLDIEVDIPTGTVHMDGFAVRYNRAPDGINWIVYIYIKEMPRADRRLYEAYLK
                     TV"
     gene            complement(2246..>2690)
                     /locus_tag="PKZ53_11810"
     CDS             complement(2246..>2690)
                     /locus_tag="PKZ53_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNJ41168.1"
                     /translation="GAWCLVLGAWYLVLGAWCLVLGAWYLVLGAWCLVLGAWYLVLGA
                     WCLVLGALEPIPSKNKERRTKNKERRTKNEEQRTKNKERRTKNQELGSGQDATRADCE
                     RQVNFRLRQPKAVATLCANVVGIQQKKHKRKKFNSPLVKPAYLVV"
BASE COUNT          638 a          689 c          679 g          684 t
ORIGIN      
        1 gattttcagc cctgaaaagt ccttcccgta ctgtgcctcc tggtcggcaa tggtttgcag
       61 gtagtcataa atcaacaacc cttttccagg tttgaccgga atcagatttg cttcaaaaaa
      121 gcgatagggg acgcttttgc gggtggttga agctttaaac ttcatcagca cctgaattgg
      181 aatgaaaaaa actttctgat gcgcgctaaa ttcgaccaga aagccagcca cagccccaac
      241 gtttgcggtg cgttccagat aactcacctg atgtggatga aggttgtcaa aatcaagctg
      301 ttcgcggctg gtgcttttgg cgtcgtaata aatcgccctt cccgttggcg caatacatcc
      361 gccgtaatcg ggttcggcac gcgattcggg gacaaactgg cgtgggtcgc gcgcgccttc
      421 cgacttgagg atactctgta aatcgcccgc actcagcctg gccccagatt ggagcacatt
      481 gagcccttca accgacggga gtgatagccc acgtcgctta ttttcacggg aaaccgtgta
      541 tttgccagga atggctttgc gggtgatgca ggccagaccc gctttttcat aggcttcgtt
      601 ggtgcggttc agagcttcct gaaatccgct gccaacatcg gcgcctcgcc gtcgcatggg
      661 gtgagacatt tgagaaacct cgggctgaag aaaacgggct gaagaaaacg ggcttggggc
      721 tgaagaaaac gggctgaaaa aaacgggttt aataccctgg gcccaatgtc ttcagcccaa
      781 tatcttcagc ccgaagtctt cagcccgaag tcttcagccc gaagtcttca gccccaggcc
      841 cgttttcttc agccctaaaa atcaaaccgt cttcaggtac gcttcataca gccgccgatc
      901 agcacggggc atttccttga tataaatgta aacaatccag ttgattccat ctggcgcccg
      961 gttgtatcgg acagcaaagc catccatgtg aaccgttccg gttggaatgt ccacctcaat
     1021 gtcgagccgg tttttaaagg ctacggtgtg atctttgaca atgttgagat ggccagttgc
     1081 cacggtaccg gtctggatgc gcatgccttg tggcgacgca tcgtggacca caccggggat
     1141 ggttcgtgat ttgttgcctt catcatcggt atcaataatg gtcaaccgga gcggcaaatt
     1201 gagcgaagat gggtcaggtt cgacttttgg cggagatgat tcagcctggg ggttcacggc
     1261 acctttgtac atcactgatt cttttttgct gggctgcacc tgttcaatcg cggcaatgac
     1321 cgccgccatt gattgcggac gataggctgg atctttggcc agcatcttca tcaccagggc
     1381 ttcaactggg ggtggaacat cagagttgag atcacgcagc ggctgagcgg ctttggtggc
     1441 atgcgccaga atgactttca tgggctgctt ttcagaaaat ggcacgcgcc cggccagcat
     1501 ttcatagaga ataatcccca ggctgtaaat atctgaccgc tggtcggttg cctgcccggt
     1561 gcattcttcc ggtgaagtat agtgaggcat ccggaaaata acggatcccg tgtcagtaac
     1621 caccgctcca ccaagtgaat ctttcaaaca ggccagccca aaatcaacca ccttgacctg
     1681 taattcacgt gtaacttcat ccgccatcaa caacagatta cctggtttga gatcgaggtg
     1741 caggactttg gcatcgtggg cgactccaag cccaattgca gactgtcgga taacttcaag
     1801 cgcccggggt gtttgaagga ttttttcttc ctcaagctca tctttcaatg ttcggccttc
     1861 gatgtattca ctgaccagat aactggcggc attgggcaac aagccatagc caaaggttga
     1921 agcgacataa ggcgaggaaa tattgatcag tcgctgtact tcctgttgaa aggtcgtttc
     1981 agcttcacgc gtggtaaata aattgggtgg aatgattttg attgaaacct ggcgctgatt
     2041 ggtcaaatcg gtggcctggt atacatcccc gaggcgtcca cggccaatca tccgaatcaa
     2101 ttgataccga ttgtgaaagg ttcgacttcc ggggagtgat tcagccaggg aagaaccatc
     2161 ctggatacag aatcgagccg agtcgtcata gcaggaattg caagttgggc attctttcat
     2221 tcgaacaaaa acctttatag aaaattcaaa ctaccaggta cgccggtttc accaggggtg
     2281 agttgaactt ttttctttta tgttttttct gctggatacc taccacgttc gcgcaaagcg
     2341 tcgcaactgc tttaggttgc cggagtcgaa aattcacttg acgctcgcaa tcggctcgcg
     2401 tagcgtcctg gccagagccg agttcttggt tctttgttct tcgttctttg ttcttcgttc
     2461 tttgttcttc gttctttgtt cttcgttctt tgtttttggt tcttcgttct ttgttctttg
     2521 aaggtattgg ttccaaagca ccaagcacca agcaccaagc accaagtacc aagtaccagg
     2581 caccaagtac caggcaccaa gcaccaagta ccaagtacca ggcaccaagt accaggcacc
     2641 aagcaccaag taccaagtac caggcaccaa gtaccaggca ccaagcacca
//
LOCUS       DAOQDO010000708         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32134_length_2687_cov_3.327128, whole genome shotgun sequence.
ACCESSION   DAOQDO010000708 DAOQDO010000000
VERSION     DAOQDO010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32134_length_2687_cov_3.327128"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PKZ53_11815"
     CDS             complement(<1..1136)
                     /locus_tag="PKZ53_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNJ41169.1"
                     /translation="MEFKRVLPFGLLMVVSCAAAFVPTSAAVVKGGPINTGDVAWMLT
                     ATALVLLMTPGLSFFYGGMVQRKNVISTMLQSFIAMGLISILWVVVGFSLAFGDSIKG
                     VIGNPLTYFMFRGVTGATHPDLSPTIPLVLFALFQLKFAIITPALITGSFAERVKFTS
                     YMLFICLFTLFIYTPLAHWTWHPQGFLRQMGVLDFAGGSVVHMSAGFAALAGAMVLGR
                     RQVHKGGDHGMPAHIPYVLLGTGMLWFGWFGFNAGSALAASELAAQAFLTTNTASAAA
                     MLAWIFFDMLKGRAPSALGACIGAVVGLVAITPAAGFVTVGASIFIGSISSVISNAAV
                     HWKSKSTLDDTLDVFPCHGVGGIVGMILTSVFADKVGLMSGQVTT"
     gene            1694..2431
                     /locus_tag="PKZ53_11820"
     CDS             1694..2431
                     /locus_tag="PKZ53_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNJ41170.1"
                     /translation="MNRLFAFYRTTTGKKAVMAITGLIGFGFVIVHMLGNLQVFLGPE
                     QLDAYAKLLKSNPLVLWGARATLLGAVAVHIVAALQLTHQSTSARPVGYQQWKPQRSS
                     FASRTMKWTGPMLFFFIVYHLLHLTNGMVHSQAFHDHQVYRTVVESFSNPLISATYII
                     AMLLLSFHLYHGAWSLFQSLGLNHPKYNPLIRSVATLGTVVVIVGNISIPTAILLNRE
                     YFERKLQKPIVQQKVHETRFQDSIRTN"
     gene            2394..>2687
                     /locus_tag="PKZ53_11825"
     CDS             2394..>2687
                     /locus_tag="PKZ53_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ41171.1"
                     /translation="MKLDSKIPSGPIEQKWDKHRFDMKLVNPANKRKFTLIVVGTGLA
                     GAAAAASLAELGYKVLCFCFQDSPRRAHSIAAQGGINAAKNYRNDGDSIYRLFY"
BASE COUNT          728 a          748 c          631 g          580 t
ORIGIN      
        1 gttgtcacct gacctgacat caggccaact ttatcggcaa acaccgaggt caggatcatt
       61 ccgacaatcc ctccgacccc gtggcaggga aagacatcga gcgtatcgtc aagggttgat
      121 tttgacttcc agtgaacagc ggcattactg atcacacttg aaatcgaacc gataaaaata
      181 ctggcaccaa ccgtcacaaa accggcggct ggcgtaatgg caaccagtcc aaccacggcg
      241 ccgatacagg cgccaagggc tgacggagca cgtcctttga gcatatcgaa gaaaatccag
      301 gccagcatgg cggcggctga tgccgtgttg gtggtcaaaa atgcctgagc cgccagttcc
      361 gaagctgcca gcgctgatcc ggcattaaat ccaaaccagc caaaccagag catgcccgtt
      421 ccgagcaaca catagggaat gtgggcgggc ataccgtggt caccgccttt gtgaacctga
      481 cgccgcccca ggaccatcgc cccagccaga gcggcaaaac ctgccgacat atgcaccaca
      541 gagcccccgg caaagtcgag cacccccatt tgccgcaaaa atccttgcgg atgccaggtc
      601 caatgcgcca gcggtgtata gataaacagg gtgaacaggc agataaacag catatagctg
      661 gtgaatttga cccgttcggc aaaagatccg gtaatcagcg cgggtgtgat aatcgcaaat
      721 ttgagctgga agagggcaaa taagaccagt ggaatcgtcg gagacaaatc cgggtgggtt
      781 gccccagtga ctccgcgaaa cataaagtag gtcaacggat tgccgataac ccctttgata
      841 ctatcgccaa aggccagact aaatcccacc acgacccaca aaatactgat caatcccatg
      901 gcaataaaac tttgcaacat ggttgagatc acattcttgc gctggaccat gcctccataa
      961 aaaaaagaaa gcccaggcgt catcaataac accagcgccg tagccgtcaa catccaggcc
     1021 acgtcaccgg tgttaattgg cccacctttg acaacagcgg cggaagttgg aacaaaggct
     1081 gccgcacaac tgacaaccat caataaccca aatggaagca cacgtttaaa ttccattatc
     1141 tgtctttcac tcctcaatct tgaatgcagt catagaaatc tcttcaacca gaaaagtaga
     1201 cgcagtccac ttgaaaagag gaagtgcccc tcaacgagcg atcatttctg gttcaaactg
     1261 gtggtctgaa aatccaaacg ggttggaata tgaagagaga acagggggaa gagatggcag
     1321 attgtacctt ccaggcccag ggagtgacaa acccgaaatc atctggattc acaccaggat
     1381 tgcatcaatg atagataaac tatcaattcg atcaatgatt tacctgtttc cattttttga
     1441 cgcaaagtgt catttcacat caaaatcccc ggcaaaaaac cacaaaaacg gcttgaatcg
     1501 gctccgactc gaatccatac tgccgttgct ctcctcgttg gcccacctca gaatgctcta
     1561 tatcaattag ttgtggaatt ctccccttaa atctgataaa caatgggttg ccctctcaaa
     1621 cacacgcttt tttctgcaac ccaaaccgat tgaattcatc cctgcaagtt tttattttct
     1681 ggagaaaaac cacgtgaatc ggctattcgc gttttatcga acgacaactg gaaagaaggc
     1741 ggtgatggca attaccggcc tgatcgggtt cgggtttgtc atcgtccaca tgctcggaaa
     1801 ccttcaggtt ttccttggcc ccgaacaact cgacgcttac gccaaactcc ttaaatccaa
     1861 tccactggtg ctctggggcg ctcgcgccac cttgcttggc gccgtggcgg tgcatattgt
     1921 ggcggcgctg caactcactc atcagagcac cagtgcgcgc ccggtcggct atcagcagtg
     1981 gaaaccacag cgatccagct ttgcctcacg caccatgaag tggaccggcc cgatgctgtt
     2041 ctttttcatc gtgtatcacc tgttgcacct gaccaatggc atggttcaca gccaggcgtt
     2101 tcacgatcat caggtctatc gcactgtggt tgaaagtttt agcaatccgc tgatttctgc
     2161 cacctatatc attgccatgc tgctgctgag cttccatctc taccacggag cgtggagtct
     2221 ttttcaatca ctggggttga atcacccgaa atacaaccca ctgattcgct cagtcgccac
     2281 cctcggtacg gtggttgtca ttgtcggaaa catctcgatc ccgacagcaa tcctgttgaa
     2341 ccgggagtac tttgaacgca agctccaaaa accaatcgtc cagcaaaaag tccatgaaac
     2401 tagattccaa gattccatcc ggaccaattg aacagaagtg ggataagcac cgctttgaca
     2461 tgaagctggt caatcccgcc aataagcgaa aattcacgct gattgtggtc ggcaccggcc
     2521 tggctggtgc ggcggcggca gcatcgttgg ctgaactggg ctacaaggtc ttgtgcttct
     2581 gctttcagga cagcccacgc cgggcgcata gcattgcggc gcaaggcggt atcaacgctg
     2641 cgaaaaacta ccgcaacgac ggcgacagca tttatcggct cttttac
//
LOCUS       DAOQDO010000709         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32136_length_2687_cov_3.114742, whole genome shotgun sequence.
ACCESSION   DAOQDO010000709 DAOQDO010000000
VERSION     DAOQDO010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32136_length_2687_cov_3.114742"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKZ53_11830"
     CDS             complement(<1..764)
                     /locus_tag="PKZ53_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ41172.1"
                     /translation="MISNSIARSICQFFLCVFSLLAISCQYQSPDSSIPQAKTGQEFL
                     TISLDKTGNVVSRKWLESQSEIVDLGDGIKLELVELPAGQFLMGMSKPEYEFYRSQSV
                     NESWLKTEYPPREISVSSFLIGKYEVTRKQWKHVAQFPKVNLDLVAIPYFENPTNETL
                     PITWVDRLEAEEFCARLSVKTGRKFRLPSEEEWEYACRAGTSTPFAFGESITSQFVNF
                     NGDSDFPTPRTDFDHGGLLPVGCLHVANAFGIYDMHG"
     gene            995..1879
                     /locus_tag="PKZ53_11835"
     CDS             995..1879
                     /locus_tag="PKZ53_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNJ41173.1"
                     /translation="MAKSAFTFGLMAVVLSLSLGLTACIPPEPPSDNKKGGTEKESST
                     DTSGGNTKAGANSNLALGNPSNAKPDTSSPANFLSERKQFAMSYNRDKGIPNWVSWHL
                     DKNDLGDVERSQFKADPELPAGWYKVTPQDYTNSGYDRGHMCPSADRDKTEKDNEAVF
                     VMTNIIPQAGGNNQGPWANLEKYSRRLVEDGNELYIICGGSGEKEKIAKGKVSVPKKT
                     WKVIVVLKNGSDDLKRINENTRVIAISMPNEEDVRTRDWQEFRTTVAAIEKDTGYKLL
                     SNLPANVQKALKSKVDKE"
     gene            2051..>2687
                     /locus_tag="PKZ53_11840"
     CDS             2051..>2687
                     /locus_tag="PKZ53_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNJ41174.1"
                     /translation="MTKTYKNIETKLIHAGEPHPKIEGAVAMPVFQSSTFEYEGTSSY
                     DDLKYIRLNNTPNHTAIHAKLAALENAEAALVTASGMAAISSALLTVLSPGGHVLAQN
                     CLYGGTHNLVTEDLGEFGLSYDFIQPDTPDEWESLVRPNTRAIYVEAMTNPLLQVIDH
                     RKVVEFARTHGLISIIDSTFASPINFRPAEIGYDLVLHSCTKYLNGHSDIVA"
BASE COUNT          765 a          664 c          633 g          625 t
ORIGIN      
        1 ccgtgcatat catagatccc aaaagcattt gccacgtgaa gacacccaac cggtaacaac
       61 cccccgtggt cgaaatcagt tcgaggagtg gggaaatctg aatctccatt gaaattaaca
      121 aattgactcg tgattgattc accaaaggca aagggggttg aagtaccagc ccggcaggca
      181 tattcccatt cctcttcgct tgggaggcga aactttcgcc cagttttgac ggatagccgt
      241 gcgcagaatt cctctgcttc caggcggtca acccaggtaa ttggaagggt ttcgtttgtt
      301 gggttttcaa aataagggat ggcaactaaa tcaagattaa cttttggaaa ttgtgcgacg
      361 tgtttccact gtttgcgggt cacttcatat tttccaatca aaaaagatga aactgaaatt
      421 tctcgtggcg gatactcggt tttgagccaa gattcattca ctgattggct gcgatagaat
      481 tcatactctg gtttcgacat ccccattaaa aattggccag ccggaagttc aaccaactca
      541 agcttgatac catcacccaa atccacaatt tcagattgcg attcgagcca tttgcgagaa
      601 actacgtttc cggttttatc caatgaaatg gtaaggaatt cctgaccggt tttggcttga
      661 gggattgatg aatctgggga ttgatactga caggaaatag ccagcaacga gaagacacac
      721 agaaagaatt gacaaataga gcgtgcaatt gaatttgaaa tcataagatt caagtctcca
      781 ggctccgagc ctttactctg agttgagttg ttcccttaga cccaaaatca aaatgattgt
      841 tccggttcaa atccggcagg tgctttgatt tttggactca accggagctt tgcgctagag
      901 tgtgaaaaat caattccctg tgcagcctca tccgtttcat ttctgcccaa ctggctgcaa
      961 accttcttct tcaaccagga tcgagacatt acctatggca aagtctgcat ttacctttgg
     1021 actgatggct gttgtattga gcctttcact tggattaacg gcctgtatcc cacccgaacc
     1081 cccttcagac aacaaaaaag gcggtacaga gaaagagtct tcaactgaca cttcaggtgg
     1141 aaacaccaaa gctggggcca attcaaacct ggcgcttggg aacccaagca atgccaaacc
     1201 ggatacgtcc agcccagcca acttcttgag cgaacgcaaa caatttgcga tgagctataa
     1261 ccgcgataaa ggaattccca actgggtttc ctggcatctg gacaaaaacg atttaggcga
     1321 tgttgaacgc agccagttta aagctgatcc tgagcttcca gcaggctggt acaaggtgac
     1381 gccacaggac tacaccaaca gtggctatga tcggggtcat atgtgcccgt cagccgaccg
     1441 ggacaaaact gaaaaagaca acgaagccgt gtttgtgatg accaatatca tccctcaagc
     1501 tggcggaaat aatcagggcc cctgggccaa tctggaaaaa tactctcgcc ggctcgtcga
     1561 agacggaaat gagttgtaca tcatctgcgg tggaagcggc gaaaaagaga aaatcgccaa
     1621 agggaaagtg tcagtgccca agaaaacctg gaaggtgatc gtggttctga aaaacggaag
     1681 cgacgatctg aaacgaatca atgaaaacac ccgcgtgatt gcgatttcaa tgccgaatga
     1741 agaagacgtc agaacccgcg actggcagga atttcgcacc acggtcgccg ccattgaaaa
     1801 agacaccggg tacaagttgc tgtccaatct tccagccaat gttcaaaaag ccctcaaatc
     1861 aaaggtggat aaggaatagg gatgaagaca acgggctgag ggctgaagac attgggctga
     1921 agacaacggg ctcggggtat tgaacccatt atcttcagcc ccgagcttgc gagtcttcag
     1981 ccccaagccc gttgtcttca gcccaatgtc ttcagcccga cgtcttcagc cctaaaaaag
     2041 agagatgacc atgaccaaaa cttacaaaaa tattgaaacc aaactcatcc acgctggcga
     2101 gccacaccca aagattgaag gtgcggttgc gatgcccgtt tttcaatcct caacgtttga
     2161 atatgagggc acaagttcgt atgacgactt gaaatatatt cgtctcaata acacacccaa
     2221 tcacacagca atccacgcca aactggcggc ccttgaaaat gccgaagccg ccctggtgac
     2281 agccagcggc atggcggcaa tttcctcagc cttactgacg gtgttgtctc ctggtggaca
     2341 cgtgctggct caaaactgtt tgtatggcgg aactcataat ctggtgactg aagacctggg
     2401 tgaatttggg ctctcgtatg attttattca gccagatact ccggacgaat gggaatcact
     2461 cgtcaggcca aatacccgtg ccatttacgt cgaagccatg accaacccgt tgctccaggt
     2521 gattgaccat cggaaggtag ttgaatttgc ccgcacccac gggttgattt cgattattga
     2581 cagcacgttt gccagcccaa tcaactttcg ccccgcagaa atcggctatg acctggtgct
     2641 gcatagctgc accaaatatc tcaacggaca ctctgacatt gtggcgg
//
LOCUS       DAOQDO010000710         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32166_length_2686_cov_2.522235, whole genome shotgun sequence.
ACCESSION   DAOQDO010000710 DAOQDO010000000
VERSION     DAOQDO010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32166_length_2686_cov_2.522235"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKZ53_11845"
     CDS             <1..344
                     /locus_tag="PKZ53_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41175.1"
                     /translation="RISPELNAQARLLMVEATAENNEDLLKAGMFAKVYVKTTNKIPV
                     VLVPVSSITSIAGLNKIFVVEQNKVVERQVKLGLRDGDMIEITEGVKKDEQIAVSNLG
                     QLSNGVAVATK"
     gene            462..1232
                     /locus_tag="PKZ53_11850"
     CDS             462..1232
                     /locus_tag="PKZ53_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ41176.1"
                     /translation="MKLKDSIVVITGASSGIGAAAARELARQGAVVVLAARRAEDLTR
                     LADEIVREGGRALAIPTDISRYADIQTLFEKTLAAYGRVDILINNAGIGGGKPLADMS
                     DVALENTVAVNLIGPARCARAAIPQMRRQGSGLILNIGSVAGEIATLGIYSATKFGLR
                     GLNDALRRELWQDNIDVVLIAPGFIQTPMTSGLKIPMPGPEAVVNAILSAIRRPRRKI
                     VVPWIYRCLMYFGKLAPWLVDRIIGSNQGRKAYTERNQ"
     gene            1410..>2686
                     /locus_tag="PKZ53_11855"
     CDS             1410..>2686
                     /locus_tag="PKZ53_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ41177.1"
                     /translation="MQKLAEICIRRPVFATMLVTSLFVIGAFAYFRLGVDLFPKVDLP
                     TVTITTRLDGASPEEVESQISKKVEEAVNTISGIDELRSVSAEGVSQVFVTFALERDV
                     DEAAQDVRDRVGRIVGDLPTDVDPPVIEKLDPDAQPVLSLAISANRSPREITDTADKM
                     IKQNLESLSGVGQVIFIGDRKREIQVVLDAQKVASYNLSIDSIKQALAAQNVEIPGGR
                     IDQGKQEIILRTLGRITQVTDFNNVILGARDGLPIYVKDVGYVEDGVEEPRSLARLDG
                     QQAVVLLIRKQSGTNTVAVVEHIKERLETIKSILPPDYRIQIAQDQSVFIESAFHSVQ
                     EHLVLGGILAAIVVLVFLGNWRSTIISAIAIPVSIISTFALMYAMGFTLNRMTMLALT
                     LSVGIVIDDAVVVLENIFRFVEEKNMSPMEAAK"
BASE COUNT          647 a          665 c          721 g          653 t
ORIGIN      
        1 cgcgcatcag cccagaatta aatgcccagg cccggttatt gatggtcgag gccactgccg
       61 aaaataatga agacctgctc aaagccggaa tgtttgccaa ggtttatgtc aaaaccacca
      121 ataaaattcc ggtcgtctta gttccagttt cttcaatcac gtccattgcc ggcctcaaca
      181 aaatctttgt cgtcgaacaa aacaaagtcg tcgagcgaca ggtcaaactg ggcctgcgtg
      241 atggcgatat gatcgaaatt accgaaggtg tgaaaaaaga cgaacagatt gccgtttcaa
      301 accttgggca actctcaaac ggggttgcag tggcaacgaa ataatacgag gttgtagtca
      361 gtagtcagta gtcagcagtt cgctaagttc ctttgattgg gttacttaac gggtttctaa
      421 tacgaacact tctttctgtt tatattgatg agggacggca tatgaaactg aaagatagca
      481 ttgtggtcat taccggggct tcaagtggaa ttggggcggc tgcggctcgt gaactggctc
      541 gacagggtgc cgtggtggtc ctggcggccc gtcgagctga ggacctgacc cggctggctg
      601 atgaaatcgt gcgtgaaggc gggcgggccc tggcgattcc gactgacatc agccgctatg
      661 ccgacattca gacactcttt gaaaaaactc tggcggctta tggtcgggtt gatattttga
      721 tcaataacgc tggaattggt ggcggaaaac ctctggctga catgtcagac gtggcccttg
      781 aaaataccgt cgccgtgaac ctgatcggac ccgcgcgttg tgcccgggca gcaattcccc
      841 agatgcgacg ccagggaagt ggtctgatcc tcaatattgg ctctgtggct ggggaaatcg
      901 cgaccctggg gatttactca gctaccaaat ttggattacg cgggctcaat gatgccctcc
      961 ggcgcgaact ctggcaggac aacattgatg tcgtgttaat tgctcccggc tttattcaaa
     1021 ccccgatgac cagtggactc aaaattccaa tgccgggccc ggaagccgtt gtcaatgcca
     1081 ttctcagtgc cattcgtcgg ccacggcgaa aaattgtcgt gccctggatc tatcgctgct
     1141 tgatgtattt cgggaagctt gccccctggc tggtggatcg aatcatcggc agcaaccagg
     1201 ggcgcaaagc ctacacggaa cgaaaccaat gacctgggct gagggtattg ggctgagggc
     1261 taagggatta agaaaaatga tccgaaaatc gcttcatacc aggctgaatg atcgggagcc
     1321 agattttccc tcagccctca gccccaagcc ctcagccttt cagaaatcct gaaccctgaa
     1381 tctccattga acgaaagcat tcaagttcta tgcaaaaact tgctgaaatt tgtattcgtc
     1441 gcccggtctt tgcgaccatg ctggtcacgt cattatttgt gattggggcc tttgcctact
     1501 ttcgactcgg tgtggatttg ttccccaagg ttgatttgcc gacggtgacc atcaccaccc
     1561 ggctggatgg tgcttcacct gaagaagttg aatcccaaat cagcaagaaa gtcgaagaag
     1621 ccgtcaacac catcagcggg attgatgaat tgcggtcggt ttcagccgaa ggggtgtctc
     1681 aggtgtttgt cacgtttgcg ctggaacgcg acgtggatga agccgcccag gatgtccggg
     1741 accgggttgg acgaatcgtt ggcgacctgc cgaccgatgt ggacccgcca gtgattgaaa
     1801 aactcgatcc tgacgctcaa ccagtcttat cgctggccat ttctgccaat cggagtccac
     1861 gtgaaatcac cgacacggct gacaaaatga tcaagcaaaa cctggaatca ctgagtggtg
     1921 tcggacaggt catttttatt ggcgaccgca agcgtgaaat ccaggtcgtc ctcgatgccc
     1981 agaaagtggc ttcctacaac ctgagtattg attcaatcaa acaggcgctg gccgcccaaa
     2041 acgttgaaat cccaggtggt cggattgatc aggggaaaca agaaatcatt ttgagaaccc
     2101 ttggccgcat cacccaggtt accgatttta acaacgtcat tctgggtgcg cgtgatggct
     2161 tgcccatcta tgtcaaagat gtcggttatg ttgaagacgg ggttgaagaa ccccggtcac
     2221 tcgcccggct tgatggtcag caggccgtgg tgttgctgat ccgaaaacaa tctgggacca
     2281 acacggtggc cgtggtcgaa catatcaagg aacggctcga aaccattaag tctattttgc
     2341 cgccagatta tcgaatccaa attgcccagg accagtcagt cttcattgaa tccgctttcc
     2401 attcggttca agaacacctt gtcctgggtg gaatcttagc cgcaattgtg gttttggtgt
     2461 ttcttggaaa ctggcgctca accattatct cggcgattgc gattccggtt tcgatcattt
     2521 ccacctttgc cttgatgtat gccatgggct ttacgctcaa ccgaatgacg atgctggcgt
     2581 tgacgctttc ggtcggaatt gtgattgacg acgcggtagt ggtgctggaa aacatcttcc
     2641 ggtttgtgga agagaaaaat atgtctccga tggaagccgc caagga
//
LOCUS       DAOQDO010000711         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32173_length_2686_cov_2.076397, whole genome shotgun sequence.
ACCESSION   DAOQDO010000711 DAOQDO010000000
VERSION     DAOQDO010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32173_length_2686_cov_2.076397"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2511
                     /locus_tag="PKZ53_11860"
     CDS             <1..2511
                     /locus_tag="PKZ53_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNJ41178.1"
                     /translation="QVNLQCISTLSFFGTDQAADRLIEKLAVFRKFQDPVSVDLTTKI
                     ALALANFESDKALETAIEFAQKNWSNEFAERFSQATLPNKVVANLTNKIRAEVKKMKF
                     SFSLLGNVDSALNLLKALAYTRSDDVANLCKEITTTLPASNTLHTKAASILSQAAVSP
                     PPIFSPDKSLNRLAIAKNIPEMALYVHEAGMTGKLRVRTVDQVDCSVDFGEGEALYAG
                     ISAQYVDQENAFFWTFLLEPRDVAQVTFGPKSGGTGTLSGLQLEKATERLIHEGLVQK
                     GEVQQIVGSEILPDSRFSQRPVGAYFLQFKDADDPAKCRLVWQALKNNPDIVTLQQTT
                     RLGKYDLYKVLFYMLKQNMIEIDAHKKSHEISSAINDSLSMLITYLQRIERKPVLFNA
                     YKAASEVCEDIVTNTQDEVVRFAMDVMRKYFWNAYATRQIFVAANLAVCQQVINLVET
                     YAKTKSADSKEALLSYIEFSFQEQDTALAPPPPPEPEPNILQKIENIEFANDAFDEFE
                     IGFDEDSINDMFGVLENQLHATGADLPTFGQDAGLTEHEEQMLMELYDNIAVAYVKPL
                     KDFIREIKLNLKINRQTSLEWIEMVEPSVNLLFSSAEKMGYTKICDSLGEMQRVFQDQ
                     KMVPDLTYLLDDAVQGLLFYYDRLSMLLPKTFALDLSDNDLNSKKEVLIVKFILKQIP
                     EINEKMMNRIILAGLSTFDKFMHSSPDEISAVTGLSKRHGEDVFMKFYQYRHIYYRHD
                     DDEYKDKFLQMFDVKFNILREIHLEIESILASNQADKEENKARVNSLKMDRQRTLWAI
                     FILLCIKEEYDLIEAVQQAVYDVRLQKLEEYLNHLTTL"
BASE COUNT          752 a          695 c          636 g          603 t
ORIGIN      
        1 caggtcaacc tgcagtgtat ttcaaccctc agtttctttg gcacggatca agcggctgac
       61 cggctgatcg aaaaattggc agtgtttcgc aaattccagg atccagtttc agttgacctg
      121 accaccaaaa ttgcactggc gctggcaaac ttcgaatctg acaaagccct ggaaaccgcg
      181 attgagtttg cccagaaaaa ctggtccaac gaatttgctg aacgcttttc acaagccacc
      241 ctgcccaaca aagtggtcgc caatctcacc aacaaaattc gagctgaagt caagaaaatg
      301 aaattttcat tttcgctgtt gggcaacgtt gattcggcgc tcaacctgct caaggccctg
      361 gcctataccc gctcggatga tgtcgccaac ctgtgcaaag aaatcaccac gaccttgccc
      421 gccagcaata cgctccacac caaagccgcc agtattttgt cccaggctgc cgtgtcgccg
      481 ccaccgatat ttagcccaga taaatcattg aatcggctgg cgatagccaa aaatattcca
      541 gaaatggcac tctatgttca tgaggccgga atgactggaa aactccgggt ccgcacggtt
      601 gatcaggtgg attgctcggt tgattttggt gaaggcgaag cgctctacgc cggaatcagt
      661 gcccagtacg ttgatcagga aaacgccttt ttctggacgt tcctgctcga accacgcgat
      721 gtggcacagg tcacatttgg gcccaaaagc ggtgggacgg gaaccttgag cggattgcag
      781 cttgaaaaag caaccgagcg cctgattcac gaaggactgg tccaaaaagg cgaggttcag
      841 caaattgtcg gcagcgagat tttgcctgac tcccgcttta gtcagcgccc ggttggcgcc
      901 tacttcctcc aattcaaaga tgctgacgat ccagccaaat gccggctggt ctggcaggcg
      961 ctcaagaaca acccggatat tgtgaccctg cagcagacga cccggctggg caaatacgat
     1021 ctctacaaag ttctgtttta tatgttgaaa cagaacatga tcgaaattga tgcccacaaa
     1081 aagtcacacg agatcagcag tgccatcaac gattcactgt caatgctcat tacctatctg
     1141 cagcgaatcg aacgcaaacc tgttttgttt aatgcctaca aagcagcctc cgaagtttgc
     1201 gaagacattg tcaccaacac tcaggacgaa gtggtcaggt ttgccatgga cgtcatgcgc
     1261 aagtactttt ggaatgcgta tgccacccgg caaatctttg tggcggccaa tctggcagtc
     1321 tgccagcagg tcatcaatct ggtggagacc tatgccaaaa ccaaatcggc tgacagcaaa
     1381 gaagcactgc tcagctatat tgaattttcc tttcaggaac aggatacggc actcgccccg
     1441 ccaccaccgc ccgagccaga accaaacatt ctgcaaaaga ttgaaaacat tgaatttgcc
     1501 aatgatgcct ttgacgaatt tgaaatcggc tttgacgaag attcaatcaa cgacatgttt
     1561 ggtgtgctgg aaaatcaact ccatgcgacc ggggctgatc ttccgacctt tggtcaggat
     1621 gccgggttga ccgaacacga agagcaaatg ttgatggagc tgtatgacaa cattgcggtt
     1681 gcctatgtca aaccgctcaa agactttatc cgtgaaatca aattaaacct caagatcaat
     1741 cgccagacat cgctcgaatg gattgaaatg gttgagccat cggtcaacct gctgtttagt
     1801 tcagccgaaa aaatggggta caccaaaatc tgtgattcgc tgggtgaaat gcagcgcgtg
     1861 tttcaagatc agaaaatggt gccggatctg acctatttgc ttgatgatgc ggtgcagggg
     1921 ctgttgtttt actatgaccg gctctcgatg ctgctcccca aaacgtttgc gcttgatttg
     1981 tcagacaacg acctgaacag caaaaaagaa gtcctgatcg tcaagttcat cctcaagcaa
     2041 attccggaaa tcaatgaaaa gatgatgaac cggatcatcc tggccggact ttcgaccttt
     2101 gacaaattta tgcacagcag cccggatgaa atctcagccg tgacgggcct ctcaaagcgg
     2161 cacggtgaag atgtgtttat gaagttctat cagtaccggc acatctatta ccgccacgac
     2221 gatgacgagt ataaggataa gttcctgcag atgtttgacg tcaaattcaa catcctgcgc
     2281 gaaatccacc tggaaattga aagcattctg gccagtaacc aggctgacaa agaagaaaac
     2341 aaggcccggg tcaactcact caaaatggac cgccagcgaa cgttgtgggc aattttcatt
     2401 ttgctgtgta tcaaagaaga atacgacctg atcgaggccg tccagcaggc agtctatgat
     2461 gtccgattgc aaaaactgga agaatacctc aaccacctga caaccctgta atcgaatgaa
     2521 aagtcagtac cacctgcgtg agtgggtggc ggaaatataa aacccacccg ctcacgcagg
     2581 tggtactgac ctttcaggga atatccgatt gagcgcccaa ctgagcaacc acatactgac
     2641 gaccaccacg cccattgaag ccagccagta ctgccagtac accgga
//
LOCUS       DAOQDO010000712         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32237_length_2683_cov_2.094749, whole genome shotgun sequence.
ACCESSION   DAOQDO010000712 DAOQDO010000000
VERSION     DAOQDO010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32237_length_2683_cov_2.094749"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKZ53_11865"
     CDS             <1..467
                     /locus_tag="PKZ53_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41179.1"
                     /translation="SNGSKGVYFQANDARKVLELSTHLYSTSGSIIIELDQTGAELQK
                     LNPGESCSERFTLQFPLYEKLLPYLVLSLPKKIDLEQIKFIQAAVGVIPFDTGIEEIL
                     NESASRICVTGNTTLKKGPFKGKNLIDLQKVIHSDLNPVSAFFQSAYPNTTK"
     gene            complement(740..1222)
                     /locus_tag="PKZ53_11870"
     CDS             complement(740..1222)
                     /locus_tag="PKZ53_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ41180.1"
                     /translation="MQKQPTPTWFFVLVVVRLGHRFLLVHERKHGQLWYLPAGRVEPG
                     ESLVQAAQRETLEEAGIPIEVDGVLRFEHSPSLEGSTRFRVIFTAHPVDDTPPKTTPD
                     ADSLEAAWLTLEEIRQLPLRGEEVVNILRQVAYGAAIYPLTILTAEGQPFLNQTRKIN
                     "
     gene            complement(1238..2086)
                     /locus_tag="PKZ53_11875"
     CDS             complement(1238..2086)
                     /locus_tag="PKZ53_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41181.1"
                     /translation="MNPEALKPVLVADQSLARRLELAEARSCAEFVETRARLFPEIQA
                     EWIEVAGAYALFDGVASPITQTFGLGLFQNVERCDLERIEAFFTSQGAPVYHEVSPLA
                     EPALLTLLNERGYHPIEFTSVMFCPIHGDIQLEVPRNERVQVRLITPDEHDLWAQTAA
                     DGFRGFQNVDDQILELQQLNARRPGAFSFLAELDGCAIGAGILYLCNGVALLGGASTI
                     PEARQQGAHVALLQARMRFAAEHGCDVAMISAQPPGGGSQRNAERHGFKIAYTRIKWH
                     LVFEGP"
     gene            complement(2183..>2683)
                     /locus_tag="PKZ53_11880"
     CDS             complement(2183..>2683)
                     /locus_tag="PKZ53_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41182.1"
                     /translation="KVIGVISGNPATGDLLGEMLSGIGNFIQWFSTAAITDQAVPGLA
                     AMEPDGDFIRNINQTQPGQPTVENSPYFAVIADFEPKLFGRHEPKEFSTRLLQWVANG
                     VVDELMRGTANDLVVDTPSMTCIDPQAGNCIKDKLDFGKTPEVYHTVYFTRKEVTDAL
                     TRWFGL"
BASE COUNT          691 a          694 c          633 g          665 t
ORIGIN      
        1 ggagtaacgg gtcgaaaggt gtttacttcc aggcaaatga tgcccggaag gttcttgaac
       61 tcagcacaca cctgtattca acttcgggtt caatcattat cgaacttgat caaaccggtg
      121 ctgaactcca aaagttaaac ccaggtgaat catgttcaga gcggttcacg ctccagtttc
      181 cgctgtatga aaagcttttg ccttatctgg tactctcact tccgaaaaaa atcgacctgg
      241 agcaaatcaa atttatccag gcagcagtcg gagtcatccc ttttgatacc gggattgaag
      301 aaattttgaa cgaatcagct tccagaatct gtgtcacggg aaacaccacg ctaaagaaag
      361 gtccattcaa aggaaaaaac ttgattgatc ttcaaaaggt catccattcc gacttgaatc
      421 cagtgtcggc tttctttcaa tcagcttatc caaacacaac taaataaaac attttgtcca
      481 cgcgtggaca aaccacttaa atctttgaat tgatttgagt tcttcaaaca ttcgcgacga
      541 gtttttgtat gtgttgcccg tggacaaaag gctaacaaat tgataccgct gtgggattga
      601 ataagaatcc agcattttca aaccacagag gacacagagg aatccacaga gaaaatcagg
      661 ttattttttt tcattcttca ttcctcttcg gttttcctct gtgttcctct gtgtcctctg
      721 tggtgagatt ttgtttccct caattgatct tcctggtttg attcagaaaa ggctgacctt
      781 cggcggtgag aatcgtcagc ggataaatcg ccgctccata ggccacctgc cggagaatgt
      841 tcacgacttc ctcgccgcgc aacgggagct gacgtatttc ctccagggtc aaccaggcag
      901 cttcgaggga gtctgcatca ggtgtggtct tcggcggagt gtcatcaact ggatgagccg
      961 taaaaatcac tctgaaacgg gtgctgcctt ccagagaagg tgagtgctca aatcgcagca
     1021 caccgtcaac ttcgattgga attccagctt cttcgagtgt ttcacgttgg gcggcctgca
     1081 ccagggactc acccggctcg actcgtcccg ccggaaggta ccataactgg ccgtgtttgc
     1141 gctcgtgaac caataaaaaa cgatgtccga gtcgaaccac caccagcaca aaaaaccagg
     1201 ttggagtcgg ctgtttttgc ataaaaacct ccaaattcta aggtccctca aacaccaaat
     1261 gccacttgat gcgggtatag gcaattttga acccgtgccg ttcggcgttg cgttgcgaac
     1321 cgcctccggg tggctgggcg ctgatcatgg cgacatcaca tccgtgttca gccgcaaaac
     1381 gcattcgagc ttgaagcagc gccacatggg caccctgctg tcgggcttca ggaattgtgc
     1441 tggcgccacc caatagcgcc acaccattac agagatacaa aatcccggcg ccgatagcac
     1501 atccatcaag ttccgccaga aatgaaaatg ccccaggacg tcgggcattg agttgctgca
     1561 attccaaaat ctggtcatct acgttctgaa atccacgaaa tccatccgct gctgtttgag
     1621 cccacagatc atgttcatcc ggtgtgatga gccgaacctg aactcgttca ttccggggga
     1681 cttccaattg aatgtcacca tgaatcggac aaaacatcac gctggtaaac tcaattggat
     1741 ggtacccgcg ttcgttcaat aaggtgagga gtgctggttc agcaagcggg ctgacttcgt
     1801 gataaaccgg tgcgccctgc gaagtgaaaa acgcttcgat ccgttccaga tcacagcgtt
     1861 ctacgttttg aaataaccca agtccaaaag tctgggtgat tggcgaagcc accccatcaa
     1921 aaagagcata agcccccgcg acttcaatcc attcagcctg aatttcagga aaaagtctgg
     1981 ctcgggtttc gacaaattcg gcacaactgc gcgcttcggc cagttcgagc cgacgtgcca
     2041 gagattgatc ggcaaccaga actggtttga gagcttcagg gttcatcgtt cgattcttcc
     2101 tcgcagataa aagctttcgg agtgctgcag cttgatgcag ctttggtata cagtgacaac
     2161 aagttgcagc actccaaaat ggttacagcc caaaccagcg cgtcaaggcg tcggtgactt
     2221 cttttcgagt aaaatagacg gtgtggtaga cctcgggggt tttgccaaaa tccagcttgt
     2281 ctttgataca gtttccagcc tgcggatcaa tgcaggtcat ggagggtgta tcaaccacca
     2341 gatcattggc cgttccacgc atcagttcgt caacgacgcc atttgccacc cattgcaaca
     2401 accgcgttga gaattctttg ggttcgtggc gcccaaacag ttttggttcg aaatcagcaa
     2461 tgaccgcaaa gtaaggacta ttttcaactg tcggctggcc cggctgggtt tgattgatgt
     2521 tccgaataaa atcgccgtcc ggctccatcg cagccaatcc tggcacggct tgatccgtga
     2581 tggccgctgt ggaaaaccat tgaatgaaat ttccaatccc ggacagcatt tctcctagca
     2641 gatcgccggt tgccggattt cctgaaatca cgccaatgac ctt
//
LOCUS       DAOQDO010000713         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32277_length_2681_cov_2.404037, whole genome shotgun sequence.
ACCESSION   DAOQDO010000713 DAOQDO010000000
VERSION     DAOQDO010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32277_length_2681_cov_2.404037"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKZ53_11885"
     CDS             <1..863
                     /locus_tag="PKZ53_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNJ41183.1"
                     /translation="GCRTNQPSAPFTAGSVNTPPGVINTERIVTIGASISESVCALGS
                     EKQIVGADDTSLFPESVARLPKVGYQRTLQPEGILSLKPTVVMVSSEAGPAETFEQIR
                     RAGVPVFVIPSDYSAEGVKNKIRQIGQILDKKAATEALVSRLDQEFSAVANLASQSLS
                     RPKVLFFYSRGQGAQMIAGTETPADAMIRLAGAENAVKTFTGYKPMTPEATVAANPDV
                     ILIPAKTMELIGGVEALIQLPGIEQTEAGKHCRVVAIDDAELLGFGPRTADALNRLVK
                     AFHPEPGKQP"
     gene            860..1942
                     /locus_tag="PKZ53_11890"
     CDS             860..1942
                     /locus_tag="PKZ53_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNJ41184.1"
                     /translation="MSAQGILPRIRGTDRFPYWPVIGVLVFLLVLVGIAALGAGAVHL
                     SWTQIIDVFAKYLGLTQSELSLSRQEEIVLVSIRLPRIVAGMVIGAALALAGAATQGL
                     FRNPLADPGLIGVSSGSALGAVVAIVAHTKVRLFLPEISGIGLPMVTAFLGGLLVSMI
                     VHQGSVRQNQPSMSRMLLVGIAVNALCSAITGWLTFQASEAQLRSITFWSLGSLANLN
                     WNITLMLLAVSLPAAVMVIRCAQPLNVLLLGEAEAHHLGINVRYVRQTVVIATALLVG
                     SSVAAVGMVAFVGLVVPHLGRLLVGPHHRILFPVAALIGAVLLPAADILARTVIAPAE
                     LPIGIVTAVMGAPFFLWLIWKNDHGV"
     gene            2163..>2681
                     /locus_tag="PKZ53_11895"
     CDS             2163..>2681
                     /locus_tag="PKZ53_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ41185.1"
                     /translation="MMLQVEAVSYQVNGISILKDISLQVDAGQVLAVIGPNGAGKSTL
                     FRLVCGDIKPNHGLIRIGGTLLDQWKPGVLAKVRAVLRQQTILQFPFTVEEVISLGLS
                     PYHHELTQTEKQSIIEKMIKLAEISHLRHRRYTTLSGGEQQRTHFARVLAQLWPAIGT
                     VPVLLMLDEPTAS"
BASE COUNT          583 a          693 c          734 g          671 t
ORIGIN      
        1 ctggatgccg gacaaaccag ccgtcggcgc cgttcacggc aggttcagta aacacacccc
       61 caggcgtaat caataccgaa cggattgtaa cgataggggc cagtatttct gaatcagtct
      121 gtgcgttagg aagtgaaaaa cagattgtcg gcgccgatga taccagcctg tttcctgaaa
      181 gcgtggcccg gttgccaaaa gttggctatc agcgaacttt acaaccagag ggaattctct
      241 cgctgaaacc aaccgtggta atggtgtctt ccgaggctgg tccagccgaa acgtttgaac
      301 aaattcgacg ggctggagtt ccggtctttg ttattccttc ggactacagc gcggaaggcg
      361 tgaagaacaa aattcgtcag attggccaaa ttctcgacaa aaaagccgcc accgaagcac
      421 tggtttcccg gctggaccag gaattctctg ccgtggccaa tcttgccagt caaagtttga
      481 gtcgtcccaa agtccttttt ttctattcgc gtggtcaggg agcacaaatg attgccggga
      541 ctgaaactcc ggcggatgcc atgattcgcc tggctggagc tgagaacgcg gtcaaaacgt
      601 tcaccgggta taagccaatg actccggaag caacagttgc cgccaatccg gacgtgattt
      661 tgatacccgc caaaaccatg gaattgatag gcggtgtcga agctctgatt cagcttcctg
      721 ggattgagca aaccgaagcc gggaaacatt gtcgagtggt ggccattgat gacgctgagt
      781 tactcggatt tggccctcga accgctgatg cgctcaaccg actggtgaag gcatttcacc
      841 ctgaaccagg gaaacagcca tgagtgcgca aggtattctg cccagaattc gaggaactga
      901 ccgctttcca tactggcctg tcattggcgt gctggtattt ttgctggtac tggttggaat
      961 tgcggcactt ggcgcaggag cggtccatct ttcctggacg caaatcattg atgtttttgc
     1021 aaaatatctt gggctcacac agagcgaatt gagtctttcg cgccaggaag aaatcgtgct
     1081 ggtctcaatc cgactgccgc gaattgtggc tggaatggtc attggtgcgg cactggcact
     1141 ggcaggggct gccacacagg ggctgtttcg aaacccgttg gcggatccgg gcttaatcgg
     1201 cgtttcaagc gggtctgcgc ttggcgccgt ggtggccatc gtggctcata ccaaagtccg
     1261 cctgttcctg ccggaaattt cagggattgg actgccgatg gtgaccgcct ttctcggcgg
     1321 gttacttgta tcaatgattg ttcatcaggg aagtgtccgc caaaatcagc cgtcaatgtc
     1381 ccgaatgctc ctggttggca ttgcggtcaa tgcgttgtgc tcagccatta ccgggtggct
     1441 cacctttcaa gccagcgaag cccagttgcg gtcaatcact ttttggagtt tgggcagcct
     1501 ggctaacttg aactggaaca tcacgctgat gttactggct gtttcacttc cggcagccgt
     1561 gatggtgatc cgatgtgccc aacctttaaa tgtactcctg ctcggggaag ccgaagccca
     1621 tcacctcgga atcaacgtcc ggtatgtcag acaaaccgtc gtgattgcca cggcgcttct
     1681 ggtcggctca agcgttgccg ccgtcggcat ggtcgccttt gtcggactgg tggtgccgca
     1741 cctgggaaga ttgctggttg gcccgcacca ccggatttta ttccctgtgg cggcgttgat
     1801 tggtgcggtt ttgctcccag ccgccgatat cctggcccgg acggtaattg cgccggctga
     1861 actcccgatt ggaatcgtga ccgccgtgat gggcgcccca ttttttctgt ggttgatttg
     1921 gaaaaatgac catggggtgt gaattttggg gttcggggtt tcaaaaccag ggttcagggt
     1981 tcagggttca gggttcaggg tcttcgaaaa aattgttttt atgtcctttt tgtcccttat
     2041 gtcctttatg tccttttctc aaaacccgga accctgacaa ggtgacaagg tgattttttc
     2101 atccctcatc cttcatccct catcccttta gaaacccgga acccgaaaga ggaaagatca
     2161 ctatgatgtt acaggttgag gccgtcagtt atcaggtcaa tggcatatca atcctcaaag
     2221 atatttccct gcaggtggat gccgggcagg tacttgccgt gattggtcca aatggcgccg
     2281 ggaaatccac gctgtttcgc ctggtgtgcg gagacatcaa gcccaatcac gggttgattc
     2341 ggattggcgg gaccttgctc gatcagtgga aacccggtgt actggccaaa gtcagagccg
     2401 tcctgcggca gcaaaccatt ctgcagtttc catttaccgt tgaggaagtt atctcgcttg
     2461 gattatctcc ctatcaccac gaattaaccc aaactgaaaa acaatccatc attgaaaaaa
     2521 tgattaaact ggccgaaatc agccatttgc ggcatcggcg ctacacgacg ctttctggtg
     2581 gtgaacagca gcgaacccat tttgcccgtg ttctggcgca actttggccg gccatcggca
     2641 cggtgcccgt tttgcttatg cttgatgagc caaccgccag c
//
LOCUS       DAOQDO010000714         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32297_length_2680_cov_2.606857, whole genome shotgun sequence.
ACCESSION   DAOQDO010000714 DAOQDO010000000
VERSION     DAOQDO010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32297_length_2680_cov_2.606857"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>2680
                     /locus_tag="PKZ53_11900"
     CDS             35..>2680
                     /locus_tag="PKZ53_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ41186.1"
                     /translation="MTEAEQTQLDQWVGTTENFPLDQCIHQLFAAQVEQTPEAIALVQ
                     IAEDDPTQIEATVTYQELNARADALASNLQALGIQPDTLVGLCLEPSIEMIVGMLGIL
                     KSGGAYLPLDPTYPGERLAFMIEDAGLSVIVTDTRSGQVLPVADQITRLNLNDLKDIR
                     DTKGINSEPGTRNPEPGTLAYAIYTSGSTGTPKGVLIEHRSLVNLACAAAQAFDLTPD
                     DAVVQFASISFDTAAEEIFPALISGARVVVKRNIRRLSFADFHRVCNTAGVTIFDPPT
                     AYWHEWTRELTRTNTALPETLRLVVVGGERALPEVFADWQQLAGNQVRWINTYGPTEA
                     TVSATLYEPATDPNFETNRTRLPIGRPLPNVQALVLDRHQQPVPPGFPGELNLGGVGV
                     ARSYLGDRGQGTGDSSKPASPAFIYPPSPIPHPLSHPPSSILHPLSHPPSPIPHPRYR
                     TGDLVRWLPDGNLEFLGRVDSQVKIRGFRVEPGEIENTLNRHQLVAESVVMVREDEHG
                     NKHLVAYVTAKSEAKSAPLSQTLSEYLEMTLPAFMMPSAILVLEAFPLTPNGKIDRKR
                     LPQFDLTGISSDAVTPLSTPTEELLAGLYAQGLKLETVGGNSHFFALGGHSLTATQLI
                     SRVRDTFAVEVPLRQLFEFPTLSGFAAVIDAARLRQASSGEQVSEVVLTRTSREHTLP
                     LSFAQERLWFLHEFEPTSTAYNLPAAFRITGPLNITALEQALIELTSRHEVLRTTFHT
                     IDGKPAQKIHSSIAENFITKKRIIGNAEYQKSKVMAEVVTEARAPFDLSRGPLLRVKV
                     FQLGETDHVLMVTLHHIISDGWSSSILVRELAALYRAFSNDLPSPLAELPVQYADFAA
                     WQRAWLQGERLDRQIAYWRTQLSDVP"
BASE COUNT          616 a          788 c          668 g          608 t
ORIGIN      
        1 cgaccctggc caacctgtaa gtacgctttc atttttgact gaagcagaac aaacccagct
       61 tgatcaatgg gttggcacaa ctgaaaactt cccgcttgat caatgtatcc accagctttt
      121 tgctgcccag gtcgagcaaa caccagaggc aatcgcactg gttcaaatcg cggaggacga
      181 cccgacccaa attgaagcaa ccgtcactta tcaggaactc aacgcccgcg ccgatgccct
      241 ggctagcaac ctgcaagccc ttggaattca accagatacc ctggtcggat tgtgcctgga
      301 accatcaatt gaaatgattg tcgggatgct cggcatttta aaatccggcg gcgcctacct
      361 gccgcttgat ccaacctacc ctggcgaacg actggcgttt atgatcgagg atgccgggct
      421 ttcagtaatt gtcaccgaca cccggtctgg ccaggtatta ccagttgctg accaaataac
      481 ccgactcaac ctgaacgact taaaggacat acgggacaca aagggcataa attccgaacc
      541 cggaacccgg aacccggaac ccggaaccct tgcttacgcc atttacacct ccggctcaac
      601 cggaacaccg aaaggtgtgc tgatcgaaca ccgctcgctg gttaatctgg cctgtgcggc
      661 tgcgcaagct tttgacctca cgcctgatga tgcggtggta cagtttgcct caatcagttt
      721 cgacacggct gccgaagaaa tcttcccggc gctgatttcc ggggccaggg tggtcgtcaa
      781 acggaacatt cggcggcttt catttgcaga ttttcaccgg gtttgtaata ccgctggcgt
      841 tacaattttt gatcctccga cggcatactg gcacgaatgg acgcgtgaac tcacccgtac
      901 aaacacggcg cttccagaaa cgttgcgatt ggttgtggtt ggtggcgagc gggctctccc
      961 cgaagtcttt gccgactggc aacaactggc tggcaaccag gtgcggtgga tcaacaccta
     1021 tggcccaacc gaagctacgg tgtcagctac actttatgaa ccagcgacag acccaaattt
     1081 tgaaaccaac cggacgcggc ttccgattgg gcgaccactt ccaaatgtgc aggcactggt
     1141 tttggatcgt caccagcaac cagttccgcc cggcttccca ggcgaactga atcttggcgg
     1201 cgtgggggtt gcgcggagct atttagggga taggggacag gggacagggg acagttcaaa
     1261 acctgcttca cctgctttca tctatcctcc atcccccatc ccccatcccc tatcccatcc
     1321 cccatcctcc atcctccatc ccctatccca tcccccatcc cccatccccc atccccgcta
     1381 ccgcactgga gatctcgttc gctggctgcc ggatgggaat ctggaattcc tgggacgggt
     1441 ggattcacag gtcaaaattc gtggcttccg ggttgaacca ggcgaaatcg aaaacacgct
     1501 caatcgtcac caactcgttg ctgaatcagt ggttatggtc cgcgaagatg agcatggaaa
     1561 caaacatctg gttgcctatg tcaccgcgaa atccgaagcc aaatcagcac ccctcagcca
     1621 aaccttgtcc gaatatctgg aaatgacgtt gccagcattt atgatgccgt cggccattct
     1681 ggtgctagag gcatttccgc taactccaaa tggaaaaatt gaccggaagc gcctgcccca
     1741 atttgatttg acagggattt catctgatgc tgttaccccg ctttccactc ccaccgagga
     1801 actgctggcg ggtttgtatg cccagggtct caagcttgaa accgttggcg gaaacagtca
     1861 tttctttgca ctcggtgggc attcattaac ggccacgcaa ctcatttcac gggtgagaga
     1921 tacgtttgcg gtcgaagttc ccctccgaca gctttttgaa tttccaacgc tgtccgggtt
     1981 tgcagcggtg attgatgcgg cccggcttcg ccaggccagc agtggtgagc aggtcagcga
     2041 ggtagtgttg acccgcactt cgcgagaaca cacgttgccg ctctcgtttg cccaggaacg
     2101 gctctggttt ttgcacgaat ttgaaccgac cagcaccgcg tacaatctgc cggcggcgtt
     2161 tcgaatcacc ggtccgctca atatcacagc cctcgaacag gcattgatcg aactgaccag
     2221 ccgtcacgaa gtcctgcgta ccacctttca cacgattgat ggcaaacctg cccagaagat
     2281 tcattcgagc attgctgaga atttcattac aaagaaacga attattggca atgctgaata
     2341 ccagaagtca aaagtaatgg ctgaagtggt gactgaagcc cgcgccccgt ttgatctttc
     2401 ccgagggcca ttgctacggg tcaaggtctt tcaacttggt gaaactgacc atgtgttgat
     2461 ggtgacgcta caccacatta tttccgatgg ctggtcaagc agcattctgg tgcgcgaact
     2521 ggcggcactc tatcgcgcct tttccaatga cctgccgtca ccgctcgctg aacttccagt
     2581 tcaatatgcc gactttgctg cctggcaacg ggcgtggctg cagggcgaac gacttgaccg
     2641 gcaaattgcc tactggcgca cccagttaag cgatgtgcct
//
LOCUS       DAOQDO010000715         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32302_length_2680_cov_2.010667, whole genome shotgun sequence.
ACCESSION   DAOQDO010000715 DAOQDO010000000
VERSION     DAOQDO010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32302_length_2680_cov_2.010667"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKZ53_11905"
     CDS             complement(<1..1043)
                     /locus_tag="PKZ53_11905"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ41187.1"
                     /translation="MFKHLQILFIITVLVSTTVWGQKTRDPFDQSNPTAPAQDQVPER
                     SEPQDRAYIGDTKPGRKPNTGRKSAKNTKTIVSPPSTLTWPDGAGRFAIIIGVNDYQS
                     EQINDLKAAVNDARLLAKTLAQNAGFEPDHIKLLTTDQGSEGQPTQGTILEKTSNFVS
                     GMPRQGLLLFFFAGHGVVNEGKAYLLPSDARMGSAALLKKTAISLEDIRNEVNQAGIE
                     QVMFILDACRNDPSAGARGNVDNLLSDDFSRGLDFRDAAFFDNKNRGIKAFVTLYATG
                     KGQRAYEDSAKAQGYFTLAIAEALTGQGNNITNQKGEVTLGNLLSYVQHKVAERVKLD
                     TGKDQVPFYDVSEG"
     gene            complement(1090..>2680)
                     /locus_tag="PKZ53_11910"
     CDS             complement(1090..>2680)
                     /locus_tag="PKZ53_11910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41188.1"
                     /translation="VPKVRDHFSDQGGQFLVMEFVPGDDFSQLFRQQYGGFPVNDVLG
                     WADQILDALDYLHTHQPPIIHRDIKPENLRLTPRGQVVLIDFGLAKETELSKSLPGFS
                     LLYTSPEQLEGQGTGPESDLYSLGATLYHFLTGCAPVDAMARTLGLARTQTDPLKPLG
                     TILPALDPQVAGVIHQAFAIERTHRWSSALEMRTALRAVMPPGLLTGRFQVPVGVGST
                     GRQLAQTGSYSPTVIPASQTSGGYSPTIVPVPAVPNNTGASPLISSTSSLTSPAPHPV
                     ANQPSVNTSNDLVPASFQSREVEIGLFQRLKRTYGLTTLLGALVGIILLGLVGYSLVK
                     PKPTSPTASQPVSATEVKMVEILQQSIEVLPDNQPPGTLQAEVYAVRRADSIRIHIRT
                     TRPGFLYVIGRDQGKWVTMLTNRPSLKNPDRTINQISTNQVFVFPSKKSIQFRSTDEP
                     RQVFQLLFSPTQIQKPLFLDQLPEYVLSEPDTEAFNRLVKSASQLSPQHSGNAAFVSL
                     QSPDNQSAQPLYFSIIEFTLQ"
BASE COUNT          667 a          619 c          696 g          698 t
ORIGIN      
        1 ccttcagaaa catcataaaa ggggacctgg tcttttccgg tatcgagttt tacccgttcc
       61 gcaactttat gctgaacata gcttaacaaa ttcccaaggg tgacttcgcc tttttgattg
      121 gtaatattgt ttccctgtcc ggtcagcgct tcggcaatgg cgagagtaaa atatccttgt
      181 gctttggctg aatcctcgta ggctcgctgg cctttacccg tggcatacaa ggtcacaaaa
      241 gctttgatcc cacggttttt gttatcaaag aaggcggcat cccgaaaatc aagtccacgt
      301 gaaaaatcat ctgagagcag attatcaaca tttccacggg cacccgccga aggatcgttg
      361 cggcaggcat ccagaatgaa catgacctgt tcgattccag cctgattgac ctcattgcga
      421 atatcttcga gtgagatggc ggttttcttc aataaggccg cactccccat tcgggcatca
      481 gacggtaaca aataggcttt tccctcgttc accactccgt gcccggcaaa gaaaaacagc
      541 aataagccct ggcgtggcat gccactcaca aaattggagg ttttttccag aatagtgccc
      601 tgagtcggct ggccttcaga tccctggtct gtagtgagca gcttgatatg atccggttcg
      661 aatccggcat tttgggccag ggttttggcc agcaaccggg catcattgac cgcagctttg
      721 agatcattga tttgttcgct ttggtaatca ttgactccaa taataatggc aaaccgcccc
      781 gctccgtccg gccaggtgag ggttgaaggt ggggaaacaa ttgttttggt gtttttggca
      841 cttttgcgac ctgtgttagg tttacgacct ggcttggtat cgccaatgta cgcccggtct
      901 tgaggttctg atctttctgg gacttgatcc tgggcaggtg ccgtcgggtt gctttgatca
      961 aatgggtctc tagttttttg cccccaaaca gttgtagaaa ccaaaacagt aattatgaat
     1021 aagatctgaa gatgtttgaa catacaccac actcctcctc ggattgaacc agccagaaat
     1081 accgaaattc tattgaagcg taaattcaat aattgaaaaa taaagtggct gagcgctttg
     1141 attgtctggt gattggagtg agacaaaagc agcatttcca ctatgttgag gtgaaagttg
     1201 agaggcactt ttgactaatc tgttgaatgc ctcagtgtca ggttcactga gcacatactc
     1261 aggtaactga tctaaaaata aaggtttttg gatttgggtt ggtgaaaata ataattgaaa
     1321 gacctggcga ggttcatctg ttgatcgaaa ctgaatactt ttctttgatg ggaacacaaa
     1381 cacctgattg gtagagattt gatttatcgt tcgatctggg tttttgaggc tgggccgatt
     1441 ggtcaacatt gtgacccatt tcccctggtc acgcccaata acatataaaa aacctggtcg
     1501 agtagttcga atatgaattc ggatgctatc cgcacgtctc acggcataaa cctcagcctg
     1561 gagggtacct ggtggttggt tgtcaggaag tacctcaatg gactgttgaa gaatttcaac
     1621 cattttgact tctgtagctg agactggttg agatgcggtg ggagaagtgg gttttggctt
     1681 gaccaaacta tacccaacca gccccaaaag aatgatccca accagtgccc caagcaatgt
     1741 tgtcagccca taagtccgtt tgagtctttg aaaaagtcca atttccactt cccggctttg
     1801 gaacgaagct ggcacgagat cattgcttgt gttgacagat ggctgattgg caactggatg
     1861 cggagccggt gatgtcaggg atgaagtgga actgatgagg ggtgaagctc cagtgttgtt
     1921 tggcacggcg ggtaccggaa ctatcgtggg cgagtacccg cctgaagttt gagaagctgg
     1981 aatgacggtt ggtgaatagc tacccgtttg tgccagttgg cgaccagtgc ttcccactcc
     2041 caccggaacc tgaaaccgac cggttaaaag ccctggtggc atcactgccc ggagggccgt
     2101 gcgcatttcg agggctgaac tccaccgatg ggtacgttcg atggcaaatg cctgatggat
     2161 cactccagca acctggggat ccagggctgg taatatcgta cccagtggtt ttaacgggtc
     2221 ggtctgggtt cgggccaggc caagggttcg ggccatggca tcaaccgggg cgcatccggt
     2281 cagaaaatgg tagagcgtcg cgcccagcga atacaaatcg ctttcgggtc cggtaccctg
     2341 gccttcgagt tgttcagggg aggtataaag taatgaaaaa ccaggcagac tttttgagag
     2401 ctcagtctct ttcgccagtc cgaaatctat caaaaccacc tgtccccgag gggttaaccg
     2461 cagattttca ggtttgatgt cgcgatggat gatcggtggc tggtgggtgt gcaggtaatc
     2521 aagggcatcc aaaatctgat cagcccatcc aagcacgtca ttgactggaa atccaccgta
     2581 ttgctggcga aacaactggg aaaaatcatc tccggggaca aattccatca ccagaaattg
     2641 cccgccctgg tcagaaaaat gatcccgaac cttcggtaca
//
LOCUS       DAOQDO010000716         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32340_length_2678_cov_3.228746, whole genome shotgun sequence.
ACCESSION   DAOQDO010000716 DAOQDO010000000
VERSION     DAOQDO010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32340_length_2678_cov_3.228746"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PKZ53_11915"
     CDS             <1..149
                     /locus_tag="PKZ53_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNJ41189.1"
                     /translation="FEWAEGLTPRFGLVEVDYQTQHRRIKPSGHLYAQICKANGLSPD
                     LVKT"
     gene            189..1103
                     /locus_tag="PKZ53_11920"
     CDS             189..1103
                     /locus_tag="PKZ53_11920"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ41190.1"
                     /translation="MAQHKLLKRLKHHYLHPAGAGWDRLGWRYLYATSKLKARAYDKA
                     FATVQNYCWFLGYPRSGHTLVGSLLDAHPEIIIAQELHALKYVDKGFSRNQLFYLCLK
                     RSEDFTKAGRIWSGYDYNIPNQWNGRFRELKVIGDKKGGASAGTLMRHPHLVSKLVET
                     LHMPMRVIHVVRNPYDNITTIKRRAAERLDRLGVDRESMSIEDAIKMYFKFSKTIQAV
                     KQEIPPQDMYDLRHEDLIANPKQTVADLCRFLGVEPTDDYLTDCASIVYASPNKSRNS
                     FKWTPEQIQIVADEIAKYPFLKGYTFDS"
     gene            complement(1184..>2678)
                     /locus_tag="PKZ53_11925"
     CDS             complement(1184..>2678)
                     /locus_tag="PKZ53_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNJ41191.1"
                     /translation="VNSVGISAMTVVCCALIGVPLAIFLSLFDFPGRRMLAVMANLPL
                     VLPPLVGVLAFLFLYDETGLATRLVMKMFHLETPPWSLRGRAAIIAVHTYSMYAYFYS
                     FTSAALERVDFSLVEAARSLGGRPWVVWRRVVLPLLSPAFAGAALLTFMTSMASFSAP
                     YLFGGGVRYLTIEIFNAYANRQFELAMAESVLLAAISIPMLIFLQRFEGTRRFASVSK
                     GTVRRRPLVSRVSQVLALSLSTIVVLFLMLPPAMLILLSVVGEGSWRTTLLPTEYTLT
                     HYHSTLTEAITYQPILNSLAMSLPAAVAAGILGSLVAYMVVRLRFPAQRFLSGLVLVP
                     WVLPGTVVALCVIAAYRTPHWYSAGQVLVGTFWILPFIYFLRTWPLATRAVQTNFEQF
                     DASQEEAARTLGAGWLYAMWRVVLPQVWPGLVNGVTLAFVTLLGEFAASVLAFVFRNR
                     PISVEIASQLRAANLGRAAVYGVVLMILMGAALGAGEVLKQKWRPKK"
BASE COUNT          763 a          737 c          655 g          523 t
ORIGIN      
        1 attttgaatg ggctgaaggg ttgacaccac ggtttgggct ggtcgaagtt gactatcaaa
       61 cccagcaccg gcgaatcaaa cccagtggtc acctctatgc tcaaatttgt aaggccaatg
      121 gtctctcacc tgacctggtg aaaacataag cccaattctt acctttgaat tcaggaaagt
      181 ccgcttcaat ggcacagcac aaacttctca aacgtctgaa acaccattac ttacacccag
      241 ccggagctgg ttgggaccgg cttggctggc gatatctgta tgccacctca aaattgaaag
      301 ccagggccta tgacaaagcc tttgccacgg tgcaaaacta ctgctggttt ctcggctacc
      361 cccgaagcgg gcacactctg gttgggtcat tgctcgatgc ccatccggaa atcatcatcg
      421 cccaggaact acatgctctg aaatatgtgg ataagggctt ttccagaaac cagctttttt
      481 acctgtgttt aaagcgctca gaagacttta ccaaagctgg tcgaatctgg tcagggtatg
      541 actacaatat cccaaaccag tggaatgggc gatttcgtga actgaaagtc attggggata
      601 aaaaaggtgg agcatcggcg ggaacgctta tgcgccaccc gcatctggtg agcaagctgg
      661 tcgagaccct gcacatgccg atgcgggtca ttcatgtggt ccgaaacccg tacgataaca
      721 tcacgaccat caaacgacgg gcagcagaac gacttgaccg acttggcgtt gatcgtgagt
      781 caatgtctat cgaagatgcc atcaaaatgt attttaaatt cagcaagacg atccaggcag
      841 tcaaacagga aattccaccc caggatatgt atgacctccg gcacgaagat ctgattgcca
      901 atcctaaaca aacagtggca gacctgtgcc gctttttagg ggttgaacca actgacgatt
      961 atctgactga ttgcgccagt atcgtgtatg cctcacccaa taagtcccgc aacagcttta
     1021 aatggacccc agaacaaatc cagatcgtcg ccgacgaaat tgccaaatac ccatttctga
     1081 agggctacac gtttgacagc taaagatttg tcagtaccac ctgcgtaagt gggtggatgg
     1141 aaacgaagcg aacccacccg ctcacgcagg tggtactgac tccttatttt ttgggtcgcc
     1201 acttttgttt gaggacttcc ccggcaccta acgcggcgcc cattaaaatc atcaaaacca
     1261 caccatagac agcggctctt ccaagattgg cggcgcgcag ttgtgaggca atttcaaccg
     1321 agatgggccg atttcggaat acaaaggcca gaaccgaagc ggcaaattcg ccaagcaatg
     1381 tcacaaaagc gagcgtcaca ccgttgacca atccgggcca gacctgtggc aacaccacac
     1441 gccacatggc atacaaccag ccagcgccaa gcgtacgggc ggcttcttcc tgtgaagcgt
     1501 caaattgttc aaagttggtt tgcacagcac gggtcgcaag cggccaggtt cgcaaaaaat
     1561 aaatgaaggg taaaatccag aacgtcccaa ccagtacctg cccggctgaa taccagtgcg
     1621 gcgttcgata ggcagcaatc acgcacagtg ccaccaccgt gcccggcaga acccacggca
     1681 caagcaccag tccagagaga aagcgttgtg ctgggaaccg taacctgacc accatatagg
     1741 cgaccagact tcccaatata cctgcagcaa cggcagccgg gagcgacatc gccagactat
     1801 tgagaattgg ttgataggta atggcttcgg tcaaggttga gtgatagtga gtcagcgtgt
     1861 attcagttgg cagcaacgtc gtgcgccagc ttccttcacc cacaacgctt aagagaatca
     1921 acatcgccgg cggcagcatc aaaaacaaaa ccacgatagt ggacagactc aaagccagca
     1981 cctgagacac ccgcgaaacc agtggtcgtc gccgaaccgt gcctttgctg acgctggcaa
     2041 accgccgtgt tccttcaaac cgctggagaa agatcagcat aggaattgag atcgccgcca
     2101 gcaaaacgct ttccgccatg gccagttcaa actggcggtt ggcataggca ttgaagattt
     2161 caatagtcaa ataccggacc ccgccgccaa acaaatacgg ggcactaaaa ctggccatcg
     2221 aagtcataaa agtcagcagg gcggcgccgg caaaggctgg actgagcaat ggcaatacca
     2281 cccgccgcca gaccacccag ggtcgcccac caaggctgcg ggcagcttca accagtgaaa
     2341 aatcaactcg ctcaagtgct gccgaggtaa agctataaaa ataggcatac attgaatagg
     2401 tatgcacggc gataatcgct gcgcgaccac gcaacgacca gggaggcgtc tccagatgaa
     2461 acatcttcat taccagccgt gtggctaatc cagtttcatc atagagaaac aaaaacgcca
     2521 ggaccccaac cagcggtggt aaaaccagtg gtaaatttgc catcacagcc agcatccggc
     2581 ggcctgggaa gtcaaagaga ctcaggaaaa tggctaatgg cactccgatc aaggcacaac
     2641 acaccacggt catcgccgaa atgccaacgc tattgacc
//
LOCUS       DAOQDO010000717         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32359_length_2677_cov_2.624333, whole genome shotgun sequence.
ACCESSION   DAOQDO010000717 DAOQDO010000000
VERSION     DAOQDO010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32359_length_2677_cov_2.624333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1055)
                     /locus_tag="PKZ53_11930"
     CDS             complement(366..1055)
                     /locus_tag="PKZ53_11930"
                     /inference="COORDINATES: protein motif:HMM:NF040041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HNJ41192.1"
                     /translation="MTHKRNVEGIRLNAKRKHQEALTRADEAIRLLVNEKRPVNFKSV
                     AETAGISIPFLYQNQALKERIIHLRSQTTTRVQLKVKAHPSDASKDAMIEALQLRAKE
                     AEAENRSLKKQLEVVYGQLLHQGTPEQPAGGDPVSSVSATATNLQHLLNQANSEKNEL
                     AVINQQLKQQNHEGQEKLLVLNEREREVEILKRQNQSLINEIILLKSGQPGASPPVTK
                     PRPKGRTKSAK"
     gene            complement(1039..>2677)
                     /locus_tag="PKZ53_11935"
     CDS             complement(1039..>2677)
                     /locus_tag="PKZ53_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ41193.1"
                     /translation="KQNLVHSLVDWMKSCLPASTRSLLERDLNDWERSYCEFRLGQSR
                     VVDKKHIFLLASQQFKECTRVNEAISFLRHLYREVEKLYDTRPEHEKDIWDFRQLGVS
                     VSAANSHFRANFTLIQQPWLRQLAKTCLYYRLAIYSVSDCLCKLNALKAFSSFLAEYY
                     PNVGPQHVTRKLMVGYFGFLLSKKLSPATRGDYIVHLRAILELSVRMGWGDLPKSQVI
                     YDEDIPRASNPVPRFIPDQVLAQIFLHLENLETRWQRMFRVAYEVGMRISELINLPFN
                     CLLQDSTGDWWIKYHQVKLKQEHTQPISKETAVLIQTQQQEIQKRFDHPPKLLFPNPK
                     GNRISGDLFRVAINRFIVEHNIRDGAGALWRFQPHQLRHSFGTRHINSGTPHLIVMRL
                     MGHRSEGMTARYAYIHDQTLKQEFEKSLSNRKLIDISGRARTEHSPADAIELQWLKKH
                     TNARTLPNGYCGRPILLEECPTPNCCLTCPHFLTDKTHLSIHENQLAETVKLVQITRS
                     KGWTVQAENNERVAQNLKTIIASLKEDTKEVSNDTQT"
BASE COUNT          668 a          615 c          620 g          774 t
ORIGIN      
        1 cgaaccagag tttcgtgcaa cagcaacgaa tactcttgct cggcggccag cgcgcggagt
       61 aaactctcca ccaggtcgcg ttttgaataa tcatagttat ctacagtgaa tttttgtaaa
      121 atctacatat acaatggatt ttactatatt tacatataaa ataagtagga ctctattcag
      181 tgagattcgg ggctcatttg gtggaaaccc cgacccttga acacgtcccg acgacaacaa
      241 gttgccgagg cgaagggaaa aaacggaaga acgtgttttt taccctttgc ctcggcaatc
      301 aattgtgcca gaacgcgcaa gggcaacggt gtttatgtgc ctcagctcag ctaaaagccg
      361 gaagctcact tggccgattt ggttctgcct tttggtcttg gctttgtaac cggcggggaa
      421 gctcccggtt gcccggattt gagcaggatt atttcgttga tgagagattg gttttgtctt
      481 ttcaggattt caacttcccg ctccctttca ttcagcacca acaatttttc ctgaccttcg
      541 tggttctgtt gtttcaactg ctgattgatg acagccaatt cgttcttttc tgagtttgcc
      601 tgattgagca ggtgttgaag gtttgttgct gttgcggaaa ctgacgaaac gggatcaccc
      661 cccgccggtt gctccggtgt tccttggtgg agcaattggc catagacgac ttcaagctgc
      721 tttttcaaac tccggttttc ggcctcagcc tcttttgctc ggagttggag ggcctcaatc
      781 atagcatcct tggaagcatc ggaaggatgc gctttcactt tgagctgaac gcgggtggtc
      841 gtttggctcc ggagatggat gatgcgttcc ttcagggcct gattctggta aagaaatgga
      901 attgagatcc cggccgtttc cgccacgctc ttaaaattga ccggtcgttt ttcgttcacc
      961 aacaggcgaa tggcttcatc cgcacgggtc aatgcctcct gatgtttgcg tttggcattt
     1021 aggcgaattc cttcaacatt acgtttgtgt gtcattgctg acttccttcg tatcttcctt
     1081 gagtgaggca ataattgttt tcaaattctg agcgactcgc tcattatttt cagcctgcac
     1141 ggtccacccc tttgagcgag tgatttgaac taacttgacc gtttccgcaa gctggttttc
     1201 atgaatcgat aaatgggttt tatcagtaag gaagtgaggg caggtcaaac agcaatttgg
     1261 agtagggcat tcctctaaaa gtattggccg cccacagtag ccatttggca aagttcgtgc
     1321 attcgtgtgt tttttgagcc attgcagttc aatggcatct gccggtgaat gctcggttct
     1381 ggcacgaccg ctaatatcaa tcaattttcg gttgctcaag cttttttcaa attcttgttt
     1441 tagggtttgg tcgtgaatgt aggcatacct tgccgtcata ccctcactcc ggtgacccat
     1501 caggcgcatt acgatcagat gaggcgtacc agaattgata tgcctggttc cgaatgaatg
     1561 cctgagttga tggggttgga aacgccacaa cgcacctgca ccatcacgaa tgttgtgctc
     1621 tacaatgaac ctattgatgg caactctgaa caaatctcca gaaatcctat tccctttggg
     1681 gtttggaaac agcaatttcg gtgggtgatc aaaacgtttc tgaatttcct gctgttgtgt
     1741 ttgaatgagg actgccgttt cctttgaaat cggctgggta tgttcttgct tgagtttcac
     1801 ctggtgatac ttaatccacc aatcaccggt cgaatcctgc aaaaggcaat taaatggcag
     1861 gttgattaat tcgctaatcc gcataccaac ttcataagcc actctgaaca ttctctgcca
     1921 gcgggtttca agattctcca gatgcaaaaa aatctgcgct aacacttggt cgggaataaa
     1981 acgcggaact gggttgctcg cacggggaat atcctcatca taaataacct gtgatttagg
     2041 tagatctccc cagcccattc ggaccgaaag ctccaggatg gctcgcaaat gaactatgta
     2101 atcccctcta gttgcgggtg acagcttctt ggaaaggaga aagccaaaat aacccaccat
     2161 cagttttcgg gttacatgct gaggaccaac atttgggtaa tactcagcta aaaaacttga
     2221 aaaagctttg agagcattca atttacagag acaatctgaa accgagtaaa tcgctaagcg
     2281 atagtagaga cacgttttag caagctgtct gagccagggt tgttgtatta acgtaaaatt
     2341 agccctgaaa tgactattgg ctgcactaac gctaacccct agctgacgaa aatcccatat
     2401 gtctttttca tgttcagggc gagtgtcata caatttctca acttcacgat ataggtgcct
     2461 gagaaaagaa atagcttcat tcacgcgtgt gcattctttg aattgctgtg aagccaaaag
     2521 aaaaatgtgt tttttatcaa ccactcgact ctgaccaagc cgaaattcgc aatatgacct
     2581 ttcccaatcg ttgagatctc gctccagaag tgatcgggtt gaagctggta gacaagactt
     2641 catccagtcc accaaactat gaaccaggtt ctgctta
//
LOCUS       DAOQDO010000718         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32437_length_2674_cov_1.525391, whole genome shotgun sequence.
ACCESSION   DAOQDO010000718 DAOQDO010000000
VERSION     DAOQDO010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32437_length_2674_cov_1.525391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKZ53_11940"
     CDS             complement(<1..584)
                     /locus_tag="PKZ53_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41194.1"
                     /translation="MDAAQHQRLSELFLAALEVDAANRANWVLNACENDRALSDQLEQ
                     LLAAHVCSAGLLDQTPVFDAGLELIARELPTQPATYPIGHRVGKYRLMRQIGQGGMGV
                     VFAAHHEGVPGGKPVAVKILKRGMDTEAIVRRFQTEYRILASLEHPFIARFYDGGTTS
                     DGLPYFVMELVSGLPIDEFCRQHRLSISDQLRLF"
     gene            complement(682..1254)
                     /locus_tag="PKZ53_11945"
     CDS             complement(682..1254)
                     /locus_tag="PKZ53_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41195.1"
                     /translation="MEFSPVGITQLLIAWSNGDETARDRLMPLVYDELRRLASRQLKR
                     ERSDHLLQTTALVHEAYLRMVDFQGVDWKNRAHFFGLAAHLMRQILVDYARARDASKR
                     GSGTVQISVAETPEILDSKNFELLALNDALESLAQLSPRQSQIVELRFFGGLNTDEIS
                     EVLGVSPRTVHGDWSVARAWLANEIRRTSP"
     gene            1781..2389
                     /locus_tag="PKZ53_11950"
     CDS             1781..2389
                     /locus_tag="PKZ53_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005196028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41196.1"
                     /translation="MAQLKFDHTTFFNAYRDEYGRLTQEQVDGLESLLTAIEDDAAIS
                     DIRWAAYMLATVKHECADRWQPITEYGSVSYFDKYDAGTELGRQLGNTQPGDGYNYRG
                     RGYVQLTGRANYAKMDGELNLGTTLIDTPDKALDPGIAYQVMSVGMRNGFFTGRKLSD
                     YISGSKCDYDNARRIINGLDQYQLIAGYAVQLQRILNQALVG"
BASE COUNT          743 a          684 c          620 g          627 t
ORIGIN      
        1 tgaaacaacc gaagctgatc cgaaatactc aaccggtgtt gccggcaaaa ctcgtcaatt
       61 ggcagccctg acaccagttc catcacaaag tagggtaatc cgtcgctggt cgtgcctcca
      121 tcataaaatc gggcgataaa gggatgctcc aaactggcca gaatccgata ctcggtctga
      181 aaccggcgca caatcgcctc ggtatccatt ccacgtttga gaattttcac cgccacaggt
      241 tttccaccgg gaacgccttc gtgatgggct gcaaacacca cacccattcc gccctggcca
      301 atctggcgca tcaaccggta tttcccaacc cggtgcccaa tggggtaagt agctggttga
      361 gtcgggagct ctcgcgcgat caattccaga ccagcgtcaa ataccggtgt ctgatcaagc
      421 aacccggcgg aacaaacatg cgcagcaagc aattgctcca actggtcaga taatgctcga
      481 tcattctcac aggcgttgag aacccaattg gctcgattgg cagcgtcaac ttcgagggct
      541 gccagaaaca attcgctcag tcgttggtgt tgtgcggcat ccataacaac cttgaatctg
      601 ggggtcaggg ttcggggaaa agacaaaaaa gatcaaaaag acataaagga cataaatttc
      661 gaaaccccgg aacccggaac cctatggcga ggttcgtcga atttcattgg ccagccaggc
      721 acgagctacg ctccaatcgc cgtgaaccgt gcgcggactg acgccaagga cttcactgat
      781 ttcatcggta ttcaaaccgc caaaaaaacg gagttccaca atctggcttt gtcgggggga
      841 aagctgggcc aggctttcga gagcatcgtt gagcgcaagc aattcaaagt tctttgaatc
      901 aaggatttca ggtgtctcag ccaccgagat ttgaacggtg ccactcccac gtttggatgc
      961 atcacgagcg cgagcgtaat ccaccaaaat ctggcgcatc aagtgggcgg ccaatccaaa
     1021 aaaatgagcc cggtttttcc aatccactcc ctgaaaatcc accatccgaa ggtaggcttc
     1081 gtgaaccaac gccgtggttt gcaacaaatg atcagaccgt tcccgcttga gctggcggct
     1141 ggctaaccgg cgcaattcat catataccag cggcatcaaa cgatctcggg cagtttcgtc
     1201 accattgctc caggcaatga gcaactgggt tatccctacg ggtgaaaatt ccaccacact
     1261 tcctccaatc aaaaaaatat ctggattgaa aaattttttg cgaatgagtt gcagtttttg
     1321 ccaggggttt gcgcatgact tagtgaatgc agaaattcaa agtgtcagtt catattcgtg
     1381 ccatcaatcc caatgtgtct tttactatct gatcctgaaa aaatacctgg atgaagcaat
     1441 aatttctttc tttttatacc agcggtaaga aaatggaatt cgcaaaaacc cggcttgtga
     1501 cacttgccag taactccatg aaatatcaca ctaactcaat cacatttttc cagtttcatc
     1561 catcctggtg cagtttgggc ctctcaactt agccaacact tctcaccggc aaaaggctct
     1621 cagcagagcc ttttctgggg aaaaggagga acagtagaaa aaagaattga atggtttaag
     1681 aaggtacaac tgacttataa tacggaaacg catggtctgc aagcgttttt gaaatcagtt
     1741 ttcgaacctt aaatcccact taaggagcac acccacggct atggcacaac tcaaatttga
     1801 ccacactact ttttttaatg catatcgtga tgagtatggc cggttaactc aggaacaggt
     1861 tgacggattg gaaagtctcc tgacggccat tgaagacgat gccgccatca gtgacatccg
     1921 ctgggcggcc tatatgctgg caaccgtcaa acacgaatgt gctgaccgct ggcagcccat
     1981 cacggaatat ggttcggtct cctattttga caaatatgat gccgggacag agcttggccg
     2041 ccagttggga aatactcagc ccggagacgg gtacaactac cgtggtcgtg ggtatgtgca
     2101 actgactggt cgcgccaatt acgccaaaat ggatggggaa ttgaatttgg gaacaaccct
     2161 gattgacacc ccggataaag ccctcgaccc aggaattgct tatcaggtga tgtcagtcgg
     2221 catgcgaaat ggctttttta cgggccggaa actcagtgac tacatttccg gttccaagtg
     2281 tgactatgac aatgcccggc gcattatcaa cggcctggat cagtaccagt tgatcgccgg
     2341 atatgccgtc cagctccaac gaatcctcaa ccaggcactg gttggataaa tcaaaccatc
     2401 caaatctcat tttatactgt tttgggctaa gggtattggg ctaagggcta agagaaatac
     2461 aatattctca atatgtgagc ctttatccca aacaaagaat tgctgcgcct cacataaaaa
     2521 agcagtcaag ttattgaatc aaataagctt agcaagctat taactactga ctactgactc
     2581 ctgactcctg actgaaaacc gatttttcag gtgaatccaa tgaacttcct ggctgaattg
     2641 gacgccctgt ggcaccactc gacgggtgat ccgc
//
LOCUS       DAOQDO010000719         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32448_length_2673_cov_3.171123, whole genome shotgun sequence.
ACCESSION   DAOQDO010000719 DAOQDO010000000
VERSION     DAOQDO010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32448_length_2673_cov_3.171123"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2585
                     /locus_tag="PKZ53_11955"
     CDS             <1..2585
                     /locus_tag="PKZ53_11955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ41197.1"
                     /translation="AALQTNLNLAGLFTLHRVRLSLKYGNSASSVTGYIAFAQILATR
                     LKQIQAAETFGRLALDLVEKLQATQFRTRTLLLYNLYIRMYWRPFRESFQPFTETFLS
                     GFETGDFEFATFALISISMGKIYIGEPLGQLEEELTRYLEISRKYRQSRAHQVFLLQR
                     QYVRNLTGQTADPCSLSLESESEESLLAHFTARRDGSFLGAIYYLKLVLLFLFGHTQA
                     VLKICETTRGYMLLTYANLSGVTLFWCGALAQLRLYDAQSATAEADWARIEAYTKELE
                     AARNTCPEMVHSKFHLVRAETHRVKGNLSSAMFEYEQAIGQARTHQLLLDEAIACEYA
                     AAFYATIDQRRIARAYLEDAWSAYSRLGATAKLRHLQETFADIPIQVTERRMSGPSLA
                     TTQATMISTSSRRSTGVLDVHTLLRAAQVLSGELKLERLLAKMIRLAMENAGAQRAVL
                     LLPRDGQWFIAAQCSGQTITVLESIALTDTRDWSEAVVNLATRTREIVLSDNAAQDRR
                     FVTDAYIQRVKPASVLCLPVQNQGKLSAILYLENQTVAGAFTAERVEVMNMLSAQAAM
                     SIENALLYANLEEKVQERTIELSRTVKQLQQSEAEIKAKNTQILDSIRYAETIQQAIL
                     KADEPVVGTVGEYFILFKPRDIVSGDFYWFQDFAEGVLVGIADCTGHGVPGALMSMIG
                     TTLLNQIVVEKGITEPGRILELLHEGIRRALRQGEAGTETQDGMDIALCQINVTHRRL
                     VFAGAKRPLYVVWNDGSFEEIPGNRKPIGGQQREEIRRYQSQELELQSGMMLYMCSDG
                     FTDQPNLERQKYGTKRLRTLLQTVAGIPVEIQHQHIEQALAAHQGSELQRDDITVFGL
                     KISL"
BASE COUNT          647 a          648 c          724 g          654 t
ORIGIN      
        1 gtgctgccct ccaaaccaac ctgaacctgg ccggtttgtt tactttgcat cgggtcaggt
       61 tgtcgctgaa atatggcaac tcagcctctt cggtgacggg ctacattgcg tttgcccaga
      121 tactggccac ccggttaaag caaattcagg cggctgaaac ctttggccgg ctggcactgg
      181 atctggtcga aaaactgcag gccactcagt ttcgaacccg caccctcctg ctctataacc
      241 tctatatccg aatgtactgg cggccttttc gcgaaagttt tcaaccattt accgaaactt
      301 tcctgagtgg gttcgaaact ggagactttg aatttgcgac gtttgctttg atctcgattt
      361 cgatgggaaa aatctatatc ggcgaaccac tggggcaact cgaagaagaa ctcacccgct
      421 atcttgaaat tagccgcaag taccgacaga gccgggcgca tcaggtgttt ctcttgcaac
      481 gccagtatgt gcggaacttg actggacaga ctgctgatcc ctgctcgctc tcacttgaat
      541 cagaatctga agagagcctg ctggcccact ttaccgctcg acgcgatggg tcgtttttag
      601 gggcgattta ttacctgaaa ttggtgctcc tgtttctatt tgggcacacc caggcggtgc
      661 tgaagatttg tgagacgaca cgtgggtata tgcttttgac ttatgccaat ttgtctggag
      721 ttacactgtt ttggtgtggc gcgctggctc aattgcggct ctatgatgcc caatcagcga
      781 cggctgaagc cgattgggca cgcattgaag cctataccaa ggaactggaa gccgctcgca
      841 acacctgtcc ggagatggtc catagcaaat ttcatctggt tcgggccgaa acacatcggg
      901 tgaaaggaaa tctaagttcg gccatgtttg aatatgaaca ggcaatcggt caggcccgca
      961 cccaccagct ccttttggat gaagcgattg cctgtgaata cgcggcggcc ttttacgcca
     1021 cgattgatca gcggcggatt gcacgggcct acctcgaaga tgcctggtcg gcctatagtc
     1081 ggcttggggc gacggccaag ctccgacatt tgcaggaaac atttgcagat attccgatcc
     1141 aggtgactga gcggcggatg agcggaccat ctttggcgac gactcaggcc accatgatca
     1201 gtacttcgtc gcggcgttcg accggcgtgc ttgatgtcca cacgctgttg cgggcggcac
     1261 aggtgttgtc gggtgaactc aagctggaac gcttactggc caagatgatc cggctggcga
     1321 tggagaacgc tggtgcccag cgggcagtct tgttgcttcc acgagatgga caatggttta
     1381 ttgctgctca atgtagcggg cagaccatca cggtcctgga atcaattgcg ttgacagata
     1441 ccagagactg gtcggaagcg gtggtcaatc tggcgacccg aacccgggaa atcgttcttt
     1501 ccgacaatgc ggctcaagac cgccgatttg tcacagatgc ttacattcaa cgggtgaaac
     1561 ctgcgtccgt gttgtgttta ccggttcaaa atcaaggcaa gctctcagcc attttgtacc
     1621 ttgaaaatca gaccgtggct ggcgccttta ccgcagaacg ggttgaagtc atgaatatgc
     1681 tctcggccca ggcggcgatg tcaattgaaa atgctttgct ctatgccaat ttggaagaga
     1741 aagttcagga gcgaactatt gaactcagtc gaacagtcaa acaactccag caatccgaag
     1801 ccgaaatcaa agccaaaaat acccagattc tcgatagtat ccgctatgca gagaccatcc
     1861 agcaggcgat tttgaaagcc gacgagcctg tggttggaac tgtgggcgag tacttcattt
     1921 tgtttaagcc gcgtgacatt gtgtcgggcg atttttactg gtttcaggat tttgctgaag
     1981 gagtgctggt tggaatcgct gattgcactg ggcatggcgt tccaggtgcg ctgatgtcaa
     2041 tgattggaac gacactgctc aatcaaattg tggtcgaaaa gggaattacc gagccagggc
     2101 gaattctgga gttactccac gaagggattc gacgggcgct ccgacagggt gaggctggga
     2161 ctgaaacgca ggatggaatg gacattgccc tgtgccagat caatgtcacc caccgccgac
     2221 tggtatttgc tggggccaaa cgcccattgt acgtggtgtg gaatgacgga agctttgaag
     2281 aaattccagg gaatcgcaag ccgattggcg ggcaacaacg tgaagaaatc cgacgctatc
     2341 agagtcagga actggagtta caatccggaa tgatgctgta tatgtgctct gatgggttta
     2401 ccgatcagcc caatttagaa cgacagaaat atgggacgaa acggttaaga acactcttgc
     2461 aaactgttgc tggaattcca gttgagatcc aacatcagca catcgaacag gcactggcag
     2521 ctcatcaagg cagcgagctt caacgggatg atatcaccgt tttcggattg aaaatcagcc
     2581 tttaactgta ctcagtacgt tcattttgtg tttgttataa tttgttacaa aacagaatga
     2641 gacattcctg tgctctctgt ggttcaaaaa agc
//
LOCUS       DAOQDO010000720         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32478_length_2672_cov_2.356133, whole genome shotgun sequence.
ACCESSION   DAOQDO010000720 DAOQDO010000000
VERSION     DAOQDO010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32478_length_2672_cov_2.356133"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKZ53_11960"
     CDS             <1..202
                     /locus_tag="PKZ53_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41198.1"
                     /translation="RSRAEEVGLKNWFYFIPHFSSLIPSIAPSPEPDFFSPWFFSPIN
                     FESNFPAFFISILNRICVLENS"
     gene            269..1246
                     /locus_tag="PKZ53_11965"
     CDS             269..1246
                     /locus_tag="PKZ53_11965"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNJ41199.1"
                     /translation="MNHPLKSILRLSGLVTVLLVLVQFPTEQPSVLAETPRRASTRST
                     SRAIPFPAPVEFREDDSRGLQIKTWINDRGPFIFLIDTGAGLTLISRSVAGQARVQAT
                     GKTLAIQGPGNSGPIRTEEAILQRLAIGGIDNPLPNRGRVAVTTSLPAGVDGILDPTE
                     AFQPLGFVLDFPNRRLGALDPVSESLRNRQSPAEGAIVRWVSDPQGRRPFVQLNGKVT
                     ALLDTGGRFGLAVSSTAASRLGINMTSQRRTTTLNDITGGGFLVHRIQPATVWLESMV
                     LHKIPTDLLENVPGDTPVLFGRDALRPFRVSFDPRSRLIEFAPAAEIED"
     gene            complement(1261..1893)
                     /locus_tag="PKZ53_11970"
     CDS             complement(1261..1893)
                     /locus_tag="PKZ53_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNJ41200.1"
                     /translation="MISISTVIFDYGNVLCQPQPFEIIQEMAGLCGIPTPQFQASYYQ
                     FRHQYDLGALDGIGYWNAFGAVAGFQISESTVEKLIALDNRSWAEPNFPVLDWVTRLQ
                     TAGIQTAILSNMPVDMRRELSSLCYWLPKCDVEVFSCELGIAKPDPAIFEHCLGHLRV
                     VPKEALFLDDRLENVKAAQALGLHGLVFESVELLGAELAGRFHLPPIFSK"
     gene            1999..>2672
                     /locus_tag="PKZ53_11975"
     CDS             1999..>2672
                     /locus_tag="PKZ53_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNJ41201.1"
                     /translation="MNILVLNCGSSSVKFQLIETDLDLIAANQDRKLAQGVIERIGGE
                     AIITTRVTGRESERQTLPVRNHKTAVEWILRWLCSDEAAIPDISTLGDVHAVGNRVVH
                     GGDYFHESVRISDDVLRKIEECIELAPLHNPANLKGIAAVRDVLGKGIPQVAVFDTAF
                     HQTLPDYAYLYAVPYQFYRRHRIRRYGFHGTSCRFVAYRYRQLLGIEREQVNIVALHL
                     GNGCSIT"
BASE COUNT          648 a          681 c          662 g          681 t
ORIGIN      
        1 aagaagtcgg gctgaagaag tcgggctgaa gaattggttt tatttcatcc ctcatttttc
       61 atccctcatc ccttcgattg ccccaagccc tgagcccgac ttcttcagcc cttggttttt
      121 cagccccatc aattttgaat caaacttccc tgcgtttttt atatcaattt tgaataggat
      181 atgtgtatta gaaaacagtt aaataattca accaaataaa cttaactgac tactgactac
      241 tgactactga ctacaaactg atattactat gaatcaccca ctgaaaagca tcttgcggtt
      301 gtccgggttg gtaacggtac ttttggttct ggtgcagttt cctaccgaac agccgtcagt
      361 actggctgaa acgccgcgcc gggcctcaac cagatcaacc tcccgtgcta ttccttttcc
      421 agccccggtt gaatttcgcg aagatgacag ccggggtctc caaatcaaaa cctggattaa
      481 tgatcgcggg ccttttattt tcttgattga caccggtgcc gggttgacct tgatttccag
      541 atccgtggcg ggtcaggcac gagtgcaggc aaccgggaaa acactggcaa tacaaggtcc
      601 aggaaactcg ggacccattc gaacagaaga agcgattctc caacggctgg cgattggagg
      661 aattgacaat ccgcttccta accgtggccg ggtggccgtg accacatcgc tgcccgccgg
      721 ggttgatggg attttggatc caaccgaggc atttcaaccg ctggggttcg tgctggattt
      781 tcccaatcgc cggctcgggg cgcttgatcc ggtttctgaa tcattgcgaa atcgtcagtc
      841 gccggcggaa ggggcaattg tgcgctgggt atcagaccca cagggtcgcc gtccatttgt
      901 tcaactcaat gggaaggtca cggccttgct tgataccggt ggacggtttg ggttggccgt
      961 atcatccacc gccgccagcc gattggggat caatatgaca tcacaacgaa gaaccaccac
     1021 gctcaatgac atcacaggcg gcggtttttt ggtccatcga attcaacccg ccaccgtctg
     1081 gctggaatca atggtccttc acaaaattcc aactgatctt ctggaaaatg ttcccggaga
     1141 cacaccagtc ctctttggac gtgatgccct gcgaccattt cgagtcagtt ttgaccctcg
     1201 cagccgactc attgagtttg cgccagcggc agaaatcgaa gattgagtca ggttcgctcc
     1261 ctactttgaa aaaatcggtg gtaagtgaaa ccgacctgcc agttctgccc cgagcagttc
     1321 aactgattca aaaacaaggc cgtgcaggcc cagcgcctgc gcggctttga cattttccag
     1381 gcgatcatca agaaacagag cttctttggg aacaacccgc agatggccca ggcaatgctc
     1441 aaaaatggcc gggtcgggtt tggcgattcc caactcacac gaaaagacct ccacatcaca
     1501 tttcggcagc cagtaacaca gactggaaag ctcgcgccgc atatcaaccg gcatatttga
     1561 cagaattgcg gtttgaatcc cggcggtctg cagccgtgta acccaatcaa gcaccgggaa
     1621 attcggttca gcccagcttc gattatcaag cgcaatcagt ttttccacgg ttgattctga
     1681 aatttgaaat ccggcaaccg ctccaaaggc attccaatac ccaattccgt ccagggcgcc
     1741 aaggtcatat tggtgtcgaa actggtaata gctggcctgg aactgcgggg ttggaatacc
     1801 gcatagaccg gccatttcct gaattatttc aaatggttgt ggctgacaaa gaacatttcc
     1861 ataatcgaaa ataacagttg agattgaaat cataggcttg tgtatcggca acggtgtgac
     1921 tcgccacctg caatttttcc tgatatgttt ggtgggcacc atttcagttc cccccacttt
     1981 attttcagga tttgtatcat gaatatcttg gttttgaact gcggcagttc atccgtcaag
     2041 tttcaactga ttgagactga tctggattta attgccgcca atcaggatcg aaagcttgcc
     2101 cagggtgtca tcgaacgcat cggcggcgaa gcaatcatta caactcgagt cacgggccgg
     2161 gaaagtgaac gtcaaacact cccagtcaga aaccataaaa ctgcggttga atggatcttg
     2221 cgctggctgt gttcggatga agctgcaatt ccagatattt caaccttagg tgatgtccat
     2281 gcggttggaa atcgagtggt tcacggtgga gactattttc acgaatcggt cagaatttcc
     2341 gatgacgtgc ttcgaaaaat agaagagtgc attgagttgg cgccacttca taacccggcc
     2401 aatctcaagg ggattgcggc ggttcgcgat gtcttgggaa aaggaattcc gcaggtggcc
     2461 gtgtttgata cagcatttca tcaaacgctc cccgactatg cctatctgta tgcggtaccc
     2521 tatcaatttt atcggcggca tcggattcgt cggtatgggt ttcatggaac gtcttgccgg
     2581 tttgtcgctt atcggtatcg gcaactgctc gggattgaac gcgagcaggt caatatcgtg
     2641 gcgctccatt tggggaatgg ttgttccatc ac
//
LOCUS       DAOQDO010000721         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32486_length_2671_cov_3.753823, whole genome shotgun sequence.
ACCESSION   DAOQDO010000721 DAOQDO010000000
VERSION     DAOQDO010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32486_length_2671_cov_3.753823"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1256)
                     /locus_tag="PKZ53_11980"
     CDS             complement(81..1256)
                     /locus_tag="PKZ53_11980"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ41202.1"
                     /translation="MTSVLYYLLLLFLGVTSLGGLVYYLLAIFSIRRLRRLQPDTAVE
                     ATIPLPPISLLKPLNGADPGLEVYLRTFFAQDYPQFEILFAVRHESDPAVPIVRNLMA
                     EFSQIPARLILTGEPPFANAKVYSMELMARAARFDILVITDSDASVRKDYLRQMAKTF
                     YPAEVGAMTNLYRGVGELDFWSKLEALGMSTEFMAGVVVAERLEGMKFALGPSMAIRR
                     ECLEKIGGFARMAEYLADDFVLGNWADGAGYKVALSTHVINHHASTTGFTRTFVHRLR
                     WNRSSRFSRPDGYVGQGFTYGLVWAIGLAVIAPFPWNLMVLGSILLIRFWLAIELGTF
                     LLQDRSVLGRLWMIPLQDVISWATWLGAFFGREIVWRQERYRLEFGGRFVPVVPRSK"
     gene            complement(1397..2554)
                     /locus_tag="PKZ53_11985"
     CDS             complement(1397..2554)
                     /locus_tag="PKZ53_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HNJ41203.1"
                     /translation="MSVFSQSRNIQRLRWLAVGLAVAGVALQIGAPHRPQLVEILYGQ
                     MVYPVLARGLRFTSTWLPFSLAELIVGLVIILALAGTGWLLRTMWTQRADWKALVKTG
                     VVNVLVAGGILLLGFQLCWGLNYSRLPVSQRLALPSHQATPAEVKNLCQFLIAEANRA
                     YAQAQTQTGPKPPGTLALSLDEVRADLEASFRRATWIGPTSQGSYSPAKPVFFSPVLS
                     YAGISGVYFPFTSEPNFNADMPGCDLPFTLAHEMAHQRGFAPEDEANFVAFLICITAE
                     QPYCQYSGYLLGARYLMGDACETRECTKELLAQFATGPQADLKAVGAFWARYRGIWQD
                     LSQRVNHTYLRANNVTAGVGSYNLVVRLITDYYHADHLADRSSPQNSISHQ"
BASE COUNT          744 a          737 c          651 g          539 t
ORIGIN      
        1 agttgttttc ctttttcgta aacacctaac tccgataatg tctattcttc attcttcatt
       61 cttcattctt cattcttcat tcattttgac cgtggcacca caggcacgaa ccggccacca
      121 aattccaggc gatagcgttc ctgtcgccac acgatttcgc gtccgaaaaa agcgccaagc
      181 cacgtggccc agctaataac atcctggagc gggatcatcc agagacgccc cagaacagat
      241 cggtcctgta ataaaaaggt gccaagttca atcgccagcc agaaccgaat cagcaggata
      301 cttcccaaaa ccatcagatt ccagggaaaa ggtgcgatca cagccagccc aattgcccac
      361 accaatccat aggtaaaccc ctgtccgaca tatccatccg ggcgcgagaa ccgcgaactt
      421 cggttccagc gcagccgatg gacaaaagtc ctggtaaatc ccgtggttga ggcgtgatgg
      481 ttgatcacat gggtggagag agctactttg taacccgcac catctgccca gtttccgagt
      541 acaaaatcat cagccagata ttcggccatg cgggcaaagc caccgatttt ttccaggcac
      601 tcgcgccgga tggccatgga cggacccagg gcaaatttca tgccttccag gcgttcagcc
      661 accaccacac ccgccataaa ctcggtactc atccccaggg cttcaagttt tgaccagaaa
      721 tcaagctcgc caaccccacg atacaggttt gtcatagcgc caacttcagc cggataaaag
      781 gtttttgcca tctgccgtaa ataatctttc cgaaccgaag catcgctatc agtaatcacc
      841 agaatatcaa accgtgccgc ccgagccatc aattccatgc tatagacctt ggcattggca
      901 aagggcggct cacccgtcaa aatcaaccgg gctggaatct gggaaaattc ggccatcagg
      961 ttgcggacaa tcggtactgc cgggtcagat tcgtgtcgaa ccgcaaacag aatttcaaat
     1021 tgtgggtagt cttgtgcaaa aaacgtccgc aaatagactt ccaaccccgg atcagccccg
     1081 ttcaagggct tgagcagcga aattggcggc agcggaatgg tcgcttcaac agcggtatca
     1141 ggttgcaatc gccgcagacg acgaatcgaa aaaattgcca gcaggtaata caccagcccc
     1201 ccaagactgg tcactcccaa aaacaacaac agaaggtaat aaagaacaga cgtcatagaa
     1261 aaaatgaaga atggaaagca tttgtcagta ccacctgcgt cagcgggtgg gataggaatg
     1321 aagaattgag aatagaaagc attctttttc aatcgtttcc gtggtcaaag tccacgacct
     1381 tcattccaac ccggcttcac tgatgtgaga ttgagttttg tggtgaagag cgatctgcaa
     1441 ggtgatcggc atgataataa tcggtgatta atcgcacgac cagattatag ctgccaaccc
     1501 cagcagtcac gttgttggcg cgcagatagg tatgattgac acgctggctg agatcctgcc
     1561 agatgccacg ataccgagcc caaaacgcac ccaccgcttt taaatcagct tgtggtccgg
     1621 tggcaaactg cgccagcaac tctttggtgc actcacgggt ctcgcaggca tcgcccatca
     1681 aataccgcgc gccaagcaga taccccgaat actggcaata gggttgttca gccgtgatac
     1741 agatcagaaa agccacaaag ttggcttcat cttcgggtgc aaatccacgc tgatgggcca
     1801 tttcatgggc cagagtgaac ggtaaatcac accctggcat atccgcattg aagtttggtt
     1861 cactggtaaa aggaaagtac acccctgaaa tgccggcata cgaaagcact ggagagaaaa
     1921 aaactggctt ggccggactg taggatcctt gagaagttgg accaatccag gtcgcacgcc
     1981 gaaaggatgc ctccaggtca gctcgtactt catccagaga aagagccagg gtgccaggtg
     2041 gttttggtcc tgtctgagtc tgggcctgag cataggcccg attcgcttcg gcaatcaaaa
     2101 actggcagag atttttcact tcggctggcg tcgcctgatg cgagggaagc gccagccgct
     2161 gactcactgg aagccgtgaa taattcaacc cccagcacag ttgaaaccca aggagcaata
     2221 ttccaccagc aaccagcaca ttcaccacac cagttttgac cagtgctttc caatcagcac
     2281 gctgtgtcca catggttcgc agcaaccacc cggtgccagc cagagccagg atgatcacca
     2341 gcccgacaat cagttcagcc agtgaaaagg gcaaccaggt actggtaaac cgaagcccac
     2401 gcgccagaac tggatatacc atctggccat acaggatttc gaccagttgc ggacggtgcg
     2461 gtgcaccaat ctggagcgca actccagcca cggccaatcc cactgccagc cagcgaagac
     2521 gttgaatatt gcgactttgc gaaaaaaccg acatagaaaa atgcctcaaa gtagggctga
     2581 aagaaccagg gctgaagaaa gcgggctgaa gacatcgggc tgaagacatc gggcttgggg
     2641 ctgaagacat cgggcttggg gctgaagaca t
//
LOCUS       DAOQDO010000722         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32556_length_2668_cov_2.986605, whole genome shotgun sequence.
ACCESSION   DAOQDO010000722 DAOQDO010000000
VERSION     DAOQDO010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32556_length_2668_cov_2.986605"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2295)
                     /gene="feoB"
                     /locus_tag="PKZ53_11990"
     CDS             complement(61..2295)
                     /gene="feoB"
                     /locus_tag="PKZ53_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNJ41204.1"
                     /translation="MSSSRPDPALSSPPELTALRTVALAGNPNCGKTTVFNALTGLRQ
                     KVGNYPGVTVEKKEGRLRVEQVDLTLVDIPGTYSLSARSLDEQVARDVLLGLMEGSTR
                     PDLIVSIVDASNLERNLYLTTQLMELGRPVIVALNMMDVAAQNGLDLDAQLLAQHLGV
                     PVIPLVASRNIGFEQLRQSLIGTLPPLPKRQWAPPREVEHEIQKLAGLLVQQGLQSEA
                     TEGEAIRLLLNDTETSPVKQELTPEVESQIAESRAYLESLDIDWWSVEAEGRYGWIKN
                     VAVAAGVVHQKFRRTTSDKIDSVLTHRVLGPVIFAVVMAVVFQSIFKWAELPMNYIDQ
                     LTGQASNWVRTLIPDGDLQGLVAEGVIPGVGGVVKFLPQILILMFFISLLEDTGYMAR
                     AAFIMDRIMRRVGLNGKSFIPLLSSFACAIPGIMATRTIENSKDRLVTILIAPLMSCS
                     ARLPVYTLMIGAFFPADQTLFGVITLAGLVLFAMYLLGIVAAISMGWLFKNTLIKSGT
                     TPLIMELPPYRLPSWRNIFSSLLSRTGQFLHRAGTVILAISMILWFLAAYPKNLVLSK
                     NYTTERQQVETLFTAQKISLQEKETQLAQLSFEETSERSRMSYLGQIGHLIEPVIAPL
                     GFNWKIGVGLVSAFAAREVLVSTLAIVYSVGDDSDEGASSLHQALRNDRYPDGRPVYS
                     PLVAVSLMVFFVLALQCMSTFAIVRRETNSWRWPFFMLGYMTLLAYLGSLTVFQGGRL
                     LGLQ"
     gene            complement(2536..2667)
                     /locus_tag="PKZ53_11995"
     CDS             complement(2536..2667)
                     /locus_tag="PKZ53_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNJ41205.1"
                     /translation="MIPGTTVRVLKFAPLGDPIELMLRGYHLTLRKEEASFIEVTLK"
BASE COUNT          713 a          680 c          675 g          600 t
ORIGIN      
        1 tccctcatcc ttcatccctc atccctttag aaacccggaa cccggaaccc ggaacattct
       61 tcattgcaat ccaagcagcc gcccaccttg aaacactgtc aatgaaccaa ggtaagcgag
      121 caaggtcata tagccgagca taaaaaaggg ccagcgccag ctattggttt cgcggcggac
      181 aatggcaaag gtggacatac attgcagcgc cagcacaaaa aagaccatga gtgaaacggc
      241 caccagtggg ctgtatacgg gtctcccatc tggatagcgg tcgttgcgca gggcctgatg
      301 caggctggag gctccttcgt ctgagtcatc gccgacgcta tagacaatgg ccagcgtact
      361 gaccaggact tcgcgggcgg caaaggctga aaccagaccg acaccgattt tccagttaaa
      421 tccaagtggg gcaatgaccg gttcaatcag gtgaccgatc tgcccaagat agctcatccg
      481 gctgcgttcg ctggtttctt caaatgagag ttgagccagt tgggtttctt tctcctggag
      541 cgaaattttc tgggcggtaa acagtgtctc aacctgttgt cgctcagtgg tgtaattttt
      601 tgaaagcacc aaatttttgg gataggctgc caaaaaccac aaaatcattg agatagccag
      661 aatgaccgtt cctgcccggt gtaaaaactg cccggtccgg ctcagcaagg aagaaaaaat
      721 attgcgccag gacggcaggc gatagggtgg aagctccata atcaacgggg tggtgccact
      781 tttgatgagg gtgtttttaa atagccagcc catcgaaata gctgccacaa ttccaagcag
      841 gtacatggca aacaacacca gcccggccag ggtgatgacg ccaaacagag tttgatccgc
      901 tgggaagaag gccccaatca tcaacgtgta caccggtagc cgggcgctgc aactcatcaa
      961 cggcgcaatc aaaatggtca cgagacgatc ttttgagttt tcaatcgttc gcgtcgccat
     1021 aatgccgggg atggcacagg caaatgaact gagcaatgga ataaaggatt ttccattgag
     1081 cccgacccgc cgcatgatcc gatccataat gaacgcggca cgggccatgt agccggtatc
     1141 ttccaggagc gagataaaaa acatcaaaat cagaatttgg ggcagaaact tcaccacgcc
     1201 gccaacgcca gggatcaccc cttcggccac cagtccttgc aggtcaccat ctggaatgag
     1261 cgttcgcacc cagttactgg cttgacccgt gagctgatca atatagttca tcggaagctc
     1321 agcccactta aagatgctct ggaaaacgac ggccatcacc acagcaaaaa tcactggtcc
     1381 aagcacacga tgggtcagca cactgtcaat tttatcgctg gtcgtgcggc gaaatttctg
     1441 gtgaactacg ccagccgcaa ccgcgacatt cttaatccag ccatagcggc cttcggcttc
     1501 aaccgaccac cagtcaatgt ccaatgattc aagataggcg cgcgattccg cgatttggga
     1561 ctcaacttcg ggtgtcagtt cctgtttgac tggggaagtt tcggtgtcat tgagcaacag
     1621 acgaatggct tcaccttccg tcgcttcaga ttgaagaccc tgttgaacca gtaaaccagc
     1681 cagcttttga atttcgtgtt caacctcgcg agggggtgcc cattggcgtt tgggaagtgg
     1741 aggaagtgta ccaatcaggc tttgtcgcaa ttgttcaaac ccaatatttc ggctggcaac
     1801 cagcgggatg actggaacgc caaggtgttg agcaaggagt tgcgcatcca gatcaagtcc
     1861 gttttgagcc gccacgtcca tcatattgag cgcgactatg acagggcgac ctagctccat
     1921 taactgggtt gtcagataca gattgcgttc caaattgctg gcatcaacga tggagacaat
     1981 caaatcaggg cgggtgcttc cttccatcag cccaagcaat acgtcgcggg caacctgttc
     2041 gtcaagcgag cgggctgaca gactgtaggt gccggggata tcaaccagtg ttaaatcaac
     2101 ctgctcaacc cgcaggcggc cttccttttt ttcgacggta acgccgggat aattgccaac
     2161 tttttggcga aggccggtta aggcattgaa cacagtcgtt ttgccacagt ttggattgcc
     2221 ggccagggcg actgttcgaa gggcagtcag ttcaggcggg gacgacaacg ccgggtcagg
     2281 acgggatgaa ctcataaaaa caactaaagc caggaaatga agaatgaaga atgaagaatg
     2341 aatttggttt ttggaaggta ttggatgcta tggcatttct tgaatgacca aagaacagat
     2401 aggctctgga aaccagttga attcaagaac tctttgcact gcgttcaact aaaaaccgct
     2461 atacaaacca ggcagtcttt tcatcctcaa ttcttcattc tcaattctta attctcaatt
     2521 cttcattctt cattcttact tgagggttac ttcaataaag gaggcttctt ctttccgaag
     2581 ggttaaatga tagccacgga gcatcaattc gatgggatca ccaagcggag caaatttcag
     2641 tacccgaacc gtcgtgccgg gaatcaaa
//
LOCUS       DAOQDO010000723         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32592_length_2667_cov_2.617917, whole genome shotgun sequence.
ACCESSION   DAOQDO010000723 DAOQDO010000000
VERSION     DAOQDO010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32592_length_2667_cov_2.617917"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>2667)
                     /locus_tag="PKZ53_12000"
     CDS             complement(83..>2667)
                     /locus_tag="PKZ53_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41206.1"
                     /translation="GKEHQPEFPADLPVLAKLFPVLRQLAPIAEASQTVDIPDSQELR
                     RRAFGALRAVLSQLAALAPLVIHIDDLQWGDVDSATLLMELVRPPDPPPMFLIAGYRS
                     EEAETSPFLNIFLPKLQDKTLIAAQVREVRVGELSLDEARKLVQAILGNDPNSADRVE
                     AIIRESAGSPFFVNELARYAQNHSISLVGTGARDTLDAVIRERVSQLPEIARRILEVA
                     AVAGQPVGRLVVKRVAEAGADEQASVILLRSQHLIRVRRAHHMEEIEPYHDRIREAVE
                     HGLDADQLKSHHYRLAVELEAFGHADPETLAVHYRDGGDRPKAAHYALVAAEQAETAT
                     AFDRAVRLYRLALELRDETADHRQLLVKLGNALANAGRGAEAAETYLKASDLTSTGLS
                     TETALELRRRAAEQFLNSGHIEKGLTYTDQVLKAVGLRLAPTPRHAMASLILRRIWLM
                     IRGFGFTERTEAEVPPEELIRLDICWSVAAGLVFVDNIRSAEFQTRYLLFALKAGEPF
                     RIARGLNLFTGGSSVTGGRSLARTQKLLEAAISMADRVQHPYTSGMVKVTAGLVAFYN
                     GGWKESIELLDQGEDILRARCTGVTWELNTSRYYALRAMFFLGDLRQLNRESAALVQD
                     ARERGDVYALAGLRLRGGYLSYLILDNVEQARLEAEEGIRQWTSQGHHIQHFFYLAAQ
                     VEIELYVGNGIKAWDMLAARWKAFSKSLLFRVQTLLIEFHFLRARSGLSAFGQETDIK
                     RQALYLKQAEADAQRIEKEHMEWCQGIPEVIRAGIAACEGRRDDAIRLLTDAETNFQN
                     ANMWLHSTAARYRRGQLLGGSKGKTLMESARVWMNEQEIKSPEHTVDMLIPGIQPKSN
                     QGTK"
BASE COUNT          563 a          752 c          770 g          582 t
ORIGIN      
        1 tttacagcta ggggtttgca ttttgaacat aaaaaagccc acccgtgagg ctcactgttc
       61 cagattgatg gctcgcgatc tgttacttcg ttccctggtt cgattttggc tgaatacccg
      121 gaatgagcat atccacggtg tgttccgggc tcttgatttc ctgttcgttc atccagacac
      181 gggctgactc catcagggtt tttccctttg agccaccgag cagttggcca cgccgatagc
      241 gggccgccgt ggaatgaagc cacatattgg cattctggaa attggtttcg gcatcggtca
      301 gcaaccgaat cgcatcatcg cggcggcctt cacaggcggc gatcccggcg cgaatgactt
      361 ccggaatccc ctgacaccat tccatgtgtt ctttttcaat gcgctgggca tcggcttcgg
      421 cctgcttgag atagagtgcc tggcgtttga tgtcagtttc ctggccaaaa gcagacaggc
      481 cgctgcgggc gcgcaggaaa tgaaattcaa tcagcagggt ttgcacccga aacaggagcg
      541 attttgagaa cgctttccac cgggcggcga gcatatccca ggctttgata ccgtttccaa
      601 catacaattc gatctcgacc tgggccgcca gataaaagaa gtgctggatg tggtgtccct
      661 ggctggtcca ctgccggatg ccttcttcgg cttcaagccg tgcctgctca acattgtcca
      721 aaatcaaata ggagagatat ccgccccgta accgcaagcc agccagggca tagacgtcgc
      781 cacgctcgcg ggcatcctgg actaaagccg ctgattcgcg attgagttgc cgcaggtcgc
      841 ccaggaaaaa catggcgcgg agggcataat agcgcgacgt gtttaattcc caggtgacgc
      901 ccgtgcagcg agcgcgcagg atgtcctctc cctgatcgag gagttcaatg gattctttcc
      961 agccgccatt gtaaaaagcc accagcccgg cggtcacttt gaccatacct gaggtgtatg
     1021 ggtgctgtac ccggtcggcc attgaaatag cggcttcgag cagtttttgg gtccgggcga
     1081 ggctccgacc tccggtcacg gacgacccac cagtaaacaa attgagcccg cgtgcaattc
     1141 gaaaaggttc ccccgctttg agggcaaaca acaggtatcg ggtctgaaat tcagccgaac
     1201 gaatgttgtc cacaaagacc aggccggcgg cgacggacca gcaaatatca agtcggatga
     1261 gttcttcggg cggaacttcg gcttcagttc gttcggtaaa cccaaaccct cgaatcatca
     1321 accagatccg tcgcaaaatc agtgacgcca tggcatgacg tggggttggt gccagcctga
     1381 gtccgactgc tttcaaaacc tgatcggtat aggtaagtcc tttttcaata tgcccgctgt
     1441 tcaaaaactg ctcggctgcc cggcggcgaa gttcgagcgc ggtttcagtt gagagcccgg
     1501 tggaggtgag gtctgacgct ttcagatagg tttcagccgc ttcggcgcca cgtccggcat
     1561 tggccagcgc gttccccagt tttaccagga gttgccggtg gtcggcggtt tcatcccgaa
     1621 gctcaagcgc caaccggtac aaccgaactg ctcgatcaaa cgccgtggcc gtttcggcct
     1681 gttcggcggc caccagggca tagtgggcgg ctttgggtcg gtctccacca tcgcgatagt
     1741 gaaccgccag cgtttccgga tcggcatggc caaaggcttc aagctcaacc gcgagccggt
     1801 aatggtggga tttgagctga tcggcatcga gtccatgttc gaccgcttcg cgaatccggt
     1861 cgtgataggg ctcgatttcc tccatgtggt gcgctcgacg aacccggatc agatgttgtg
     1921 agcggagcaa gatcaccgac gcctgttcgt cggcgccggc ttcagccacg cgcttgacca
     1981 ccagtcggcc cacgggttgg cccgccaccg ctgccacttc gagaatccgg cgggcgattt
     2041 caggcaactg gctgacccgt tcgcggatca cggcatcgag cgtgtcgcgg gcgccagttc
     2101 caaccagtga aatcgaatgg ttttgggcat agcgggcgag ttcattgaca aaaaatgggc
     2161 ttccggctga ctcgcggata atcgcttcga cccgatcagc cgaatttgga tcattgccaa
     2221 gaatggcctg taccagcttg cgcgcttcat ccagtgaaag ctctccgacg cggacctcgc
     2281 gcacctgagc ggcaatcaag gttttatcct ggagctttgg taaaaaaatg ttcaaaaatg
     2341 ggctggtttc cgcttcttca cttcggtagc ccgcgatgag aaacatcggt ggtgggtctg
     2401 gcgggcgaac cagttccatc aaaagggtgg cgctatcaac gtcgccccat tgcagatcat
     2461 caatgtggat caccagcggg gccagggccg ccagttggga taaaacagca cgcagggcgc
     2521 caaatgcccg gcggcggagt tcctgggaat ctggaatatc aacggtctgg ctggcttcgg
     2581 caatcggagc aagctggcgt aagactggaa agagcttggc cagtaccggc aggtcagccg
     2641 gaaactcagg ttgatgctct tttcccc
//
LOCUS       DAOQDO010000724         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32710_length_2662_cov_2.613732, whole genome shotgun sequence.
ACCESSION   DAOQDO010000724 DAOQDO010000000
VERSION     DAOQDO010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32710_length_2662_cov_2.613732"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1652
                     /locus_tag="PKZ53_12005"
     CDS             135..1652
                     /locus_tag="PKZ53_12005"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNJ41207.1"
                     /translation="MNPQTPNPEPDFSADAASIRFKMNKQPPQVTSGIKSHLAQSFQK
                     VRPKYLLCLLAVWLLALSMSVSGQKERPRVQLKSGKVVGVESRKAATSGKVMIVSDPP
                     EATVLIDGRAVGKTAADGSLKKPVVLPAGKHQIEITRAEYEPWRQEMIITGGETRPVR
                     ASLKARFGWLQLNFNDAATEAQIQIDHQPIATSALIRHTDQAFRLKVAPGEHQVQVTQ
                     PGKTVFTTPVTIEVDQPTVVMVALRSEPAVLLVKSLPGARVYVNGLDIGIIPTTGMLK
                     TTVSPDIPAQIHLEQDGYEPFQTSVTLSAGEQTSLDATLTLLPTTVEFSDSFQEGLKY
                     WNAPKEWTADQELVRVQGENTFGTPRNKRFCDSDVVFGLRLVDGKGAAWAIRIQNETN
                     YYLFCLNGPDGHSPNKLQTFLCRNGKLDLNDALIPAQPIIPALKVGEDYRVRIRIQGN
                     RVQHWITSAQTGEESSIGFFEDGLNWFPCGNIGFATPNGETFLINGFTVRPIQNK"
     gene            complement(1640..>2662)
                     /locus_tag="PKZ53_12010"
     CDS             complement(1640..>2662)
                     /locus_tag="PKZ53_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41208.1"
                     /translation="FFVGSGFGAGITLPARDLQSPLTHQYALSFEHVIAHNLVASLAL
                     VGTHGRKLLRVRTPNGGQFSPIGLRLAPNADPLVVPLLNRPNPDLGPVTTFENSATSD
                     YHALQASVVQRFHRGFQFQIAYTYAHALDDVSDIFDVAGSSSLAQDETERSSSLRTER
                     ASANFDVRHRLTSAWVWSLPFRKWKVAQGIEWSGIVTVQTGQPFTVITSLDPNLDGNR
                     TDRLATTTGLLIHNQGRTRLSLISGLDPLTLVVPFNPLSPINGQVGRNTFRAAGVATV
                     DLALTKSIGLPNQHTLGLRIEVFNLMNRTHYGVPVRVLESPAFGSSVTTSVPARTIQF
                     ALRYLF"
BASE COUNT          615 a          685 c          718 g          644 t
ORIGIN      
        1 gttccgggtt ccgggttccg ggttccgggt tccgggttcc gggttttcga aaactttgtt
       61 cttttcgtcc tttttgtcct ttttgtcctt tttgtccttt ttgtcctttt tgtccttttt
      121 gtccttttcg tcctttgaac cctcaaaccc caaaccccga acccgatttt tcagccgatg
      181 cggccagcat tcgatttaaa atgaacaagc aaccaccaca ggtgaccagt gggatcaagt
      241 ctcatctggc tcaatccttc caaaaagtac gtccgaaata ccttttgtgc ctgctggccg
      301 tctggctcct ggcactgagt atgtctgtat ctgggcagaa agaacgtccc cgcgtccagt
      361 taaaatcagg caaagtggtt ggtgtggaga gccgcaaagc tgccacatca ggcaaagtga
      421 tgattgtttc agacccaccg gaggcaaccg tcctgattga tggccgtgcg gttgggaaaa
      481 cagcggctga tggctcactc aaaaaaccgg ttgtgctccc cgccggtaag caccagattg
      541 aaatcaccag ggctgaatat gaaccctggc ggcaggaaat gatcatcaca ggcggtgaaa
      601 ccagacctgt cagagccagc ttgaaagcca ggtttgggtg gttgcaactc aattttaatg
      661 atgctgccac ggaagcccag attcagattg atcaccagcc gattgcaact tcagccttga
      721 ttcgccacac cgaccaggca ttccgtctca aagtcgcacc cggtgagcat caggtgcagg
      781 ttacccagcc tggaaaaacc gtgtttacaa ctccggtgac cattgaggtt gaccagccaa
      841 ccgtggtgat ggttgcgctt cgttctgaac cggcagtgct gctggtcaag tcgcttcctg
      901 gtgcccgggt ttatgtcaat ggtttggata ttggcattat tcccaccact ggtatgctca
      961 agacgacggt ttccccagat attccggcgc aaatccatct tgagcaggat gggtacgaac
     1021 cttttcaaac ctccgtcacc ctgtcggctg gggagcaaac ttcgctggat gccaccttga
     1081 cgctgttgcc caccacggtg gaattttccg attcgtttca ggaagggtta aaatactgga
     1141 atgctccgaa agaatggacg gcagaccagg aactggttcg ggttcagggg gaaaatacgt
     1201 ttggcacacc gcgcaacaaa cggttttgtg attctgatgt ggtgttcgga cttcggttgg
     1261 tagacgggaa gggggccgcc tgggcaatcc ggattcaaaa tgagacaaat tactatttat
     1321 tttgtctgaa tggccctgat ggacacagtc caaacaaact ccagacgttt ttgtgccgca
     1381 atggcaaact tgatctgaat gacgctttga ttccagccca gccaattata ccggcgttga
     1441 aagttggtga agattaccgg gttcgcattc gcatccaggg aaatcgagtt cagcactgga
     1501 ttacttcggc gcaaaccggt gaagagagtt ccatcgggtt ttttgaggat gggctgaatt
     1561 ggtttccctg cggaaatatc gggtttgcca cccccaacgg cgaaaccttt ctgatcaatg
     1621 gctttactgt cagaccaatt caaaacaaat aacgcaacgc gaattgtatc gtccgggctg
     1681 gaacggacgt ggtgaccgaa cttccaaacg caggtgactc cagcacgcga accggcacgc
     1741 catagtgggt tcgattcatc agattaaata cctcgattct caggcccagt gtgtgctgat
     1801 tgggaagtcc aattgatttt gtcaacgcca gatcaacggt tgccacgcct gctgctcgaa
     1861 aggtattgcg tccaacctgg ccgttgatgg gcgaaagcgg attgaacgga accaccaatg
     1921 tcagcggatc caggcccgaa atcagagaca agcgtgttcg gccttgattg tggatcaaaa
     1981 gtccagtggt ggttgccagc cggtcggttc ggttaccatc cagatttgga tcaaggctgg
     2041 tgatgaccgt aaatggctgc ccggtttgca cggtcacaat gccagaccat tctatcccct
     2101 gggcgacctt ccattttcga aagggaagcg accataccca ggccgacgtc agccgatgtc
     2161 gcacgtcaaa atttgcgctg gcgcgctcag ttcgcagtga cgaactccgt tccgtttcat
     2221 cttgtgccag tgacgaactt cctgccacgt caaaaatatc tgacacatca tccagggcat
     2281 gcgcataggt gtaggcgatc tgaaactgga agcctcgatg aaaccgttgc actacggagg
     2341 cttgcaaggc gtgataatcg ctggtggctg aattctcaaa ggtagttact ggtcccaggt
     2401 ccggattggg gcgattcagt aacggcacca cgagcggatc ggcattgggt gccagccgga
     2461 ggccaatcgg tgaaaactgg cctccgttgg gggttcgtac ccgcaggagt tttcgcccgt
     2521 gggtaccgac cagcgccagg cttgccacca gattgtgagc gatgacgtgc tcaaacgaca
     2581 gggcgtactg atgggtcaat ggggattgta agtctctggc tggcaatgtg attccagcgc
     2641 caaagcccga tccgacaaag aa
//
LOCUS       DAOQDO010000725         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32722_length_2661_cov_2.987337, whole genome shotgun sequence.
ACCESSION   DAOQDO010000725 DAOQDO010000000
VERSION     DAOQDO010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32722_length_2661_cov_2.987337"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PKZ53_12015"
     CDS             complement(<1..1403)
                     /locus_tag="PKZ53_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ41209.1"
                     /translation="MFQKVLFGLGCVCLSGAMSLTGQIISQPSPPRAGGPAQSAVSSL
                     ASNVVIPQTSRVFSTTSARYAPVEISDIEANISLIEQVASTTLTIALKNNSSVPQQTE
                     LVVPVPDGAIIRSLTLEPVAKSGEPTPPPAKLTGKVLPREEAKTLYLSIVNKMRDPAL
                     VEFAGYNLIRSNVFPVPASGNVKLKLGFEHLCKVDGDRVDYVLPRSERLEAAQIPWKI
                     EAVIKSQKTIATVYSPSHPLTTEQSDPGLVFARTAGDGKGIPGPFRLSYLLEGKGLSA
                     TLLAYPNPNGQGGYFMLLAGVPITARSMISSEIPSLVRETTFVVDRSGSMGGEKINQA
                     RAAALQVLESLPMGEAFNIVDFSDQVERFSPKPVSKTAETLKLARDYISGIQSNGGTN
                     IDGALKMALKQPVSASVSVPVIIFLTDGCPTVGETQEAKIRQNAIEANQSKRRIFTFG
                     VGYDVNSPLLDGLATDSR"
     gene            1746..2333
                     /locus_tag="PKZ53_12020"
     CDS             1746..2333
                     /locus_tag="PKZ53_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41210.1"
                     /translation="MFHVLLCALALWFPFSTPSQKHRDHLNPQEADLVREAQEIDRRT
                     EVFLKIADRRLKALEVLADPTKAEAPLPTDPKKAKKEQERRAEEAEKWGPPPTGTRAE
                     LLANYQHVIEELEDKLDDAFDRDPKNALLNKAVGILKNGLEAEMNRLLLLRKSYGTSL
                     TREEDRAMTTAYTIAQEALNGARTYLEQNPAPKKQ"
BASE COUNT          677 a          666 c          671 g          647 t
ORIGIN      
        1 cgcgaatcgg ttgccagccc gtcgagcaat ggcgaattga cgtcataccc aacaccaaaa
       61 gtaaaaattc ggcgtttgga ctgattggct tcgattgcgt tctgacgaat ctttgcttcc
      121 tgggtttcac cgacggttgg gcagccgtcc gtcaagaata tgatcaccgg cactgagact
      181 gaggcggaaa cgggttgttt caacgccatt ttcagtgccc catcaatatt ggtcccccca
      241 ttactttgga ttccagaaat atagtcccgg gccagtttta aggtttcagc cgtctttgag
      301 actggttttg ggctaaagcg ctcaacctga tcggagaaat caacgatgtt gaaggcttca
      361 cccatcggca gagattccaa cacctggagt gccgccgccc gggcctgatt gatcttttct
      421 ccgcccatgc tccccgagcg gtccaccaca aacgtggttt ccctgacgag tgaaggaatt
      481 tccgaactga tcattgatct ggccgtgatt ggaactccgg ccagcagcat aaaatagcca
      541 ccctggccat ttggattcgg ataggccagc aatgtcgctg aaagcccttt tccttcaagt
      601 aaatatgaca atcgaaatgg tccgggaatg ccttttccat cgccggcggt gcgagcaaaa
      661 accagtccag ggtctgattg ttcggtagtg agaggatgtg aaggtgaata aaccgtggca
      721 attgtttttt gggatttgat caccgcttcg attttccagg gaatttgagc ggcttcgagt
      781 cgctcacttc gcggcagcac ataatccacc cggtcaccat caactttgca caggtgttca
      841 aagcccagct tgagttttac gttaccgctg gcggggactg gaaagacgtt tgatcgaatg
      901 agattatatc cggcaaattc gaccagcgct ggatcacgca tcttattgac aatagacagg
      961 tacagggttt tggcttcttc cctgggcaac acttttccgg tgagtttcgc cggtggcggt
     1021 gttggttctc cgctttttgc aaccggctcc agggttaaag atcggatgat ggcgccatcc
     1081 ggtacaggta ccaccagctc cgtttgttgt ggaacagaac tgttattttt gagcgcaatg
     1141 gttaaggtcg ttgaggcaac ctgttcaatc aaggaaatat ttgcctcaat gtcgctgatt
     1201 tcgaccggag catatcgcgc tgaggtggta ctgaaaactc gcgaggtctg tggaattact
     1261 acattggagg caagtgagga gaccgcactt tgagcgggtc caccagctct gggtggagac
     1321 ggctgactga tgatttgtcc ggtcaatgac attgcccccg acaggcaaac acaacccaac
     1381 ccaaataaca ctttttgaaa catatatggg ccctttgaat gataaaacat cgacaggttg
     1441 atcattccca agattcacgt gagcgtgaga cttgagtatt ttgcgaggca tgacactcag
     1501 gcggagacac cagacaggct gaaagtgctg aagtattatt tttcacgtgg attcagattg
     1561 agagtttcaa aaccttgaaa acgatgctgg cttttcgact ggagcgaagt gattttttct
     1621 ggtggcaggt ttttacagtt ttgactttca agtcaacccc tctgacgggc aacacattac
     1681 ggtgcgaaag actctggtgt ggtatcttca atttacttca cctccacatt tcaaaggatt
     1741 gatctatgtt ccatgttttg ctttgcgccc tggctttgtg gtttcccttt tccacacctt
     1801 cccaaaaaca ccgtgaccat ctcaatccgc aggaagccga tctggtccgt gaagcccagg
     1861 aaattgaccg ccgaaccgaa gtctttttga aaattgctga ccggcgcttg aaagctctgg
     1921 aagtactggc cgatccaacc aaagccgaag ccccattgcc aacggatccc aaaaaagcca
     1981 aaaaagaaca ggaacgccgg gccgaagagg ctgaaaagtg gggaccgccg ccgactggaa
     2041 cacgagccga actcctggcc aactaccagc acgtcatcga agaactcgaa gacaaactgg
     2101 atgatgcttt tgaccgtgac cccaaaaatg ccttgctcaa caaagcagtt ggaatcttaa
     2161 agaacggtct ggaagccgag atgaaccggt tattgctgct ccgaaaatcc tatggcacca
     2221 gtctgacccg cgaggaagac cgcgccatga ccacggccta caccattgct caagaagcct
     2281 taaacggcgc ccgaacctat ctggagcaaa acccagcacc aaaaaaacag taggaagcga
     2341 aacagtgacg gagtaagatc accagcaatc ttgacggaac acaaagattc cgatttcaat
     2401 tcagtttgtg ggaaaaattt ggagtgcggt gacttgtcac tgctttggta ttgatccagt
     2461 tctcagcgac attttgctga gagagcaagc gcctgtatcc aattgcggcg tttacccaca
     2521 aatgatcgag aggaaagcct atgctccgta ttgcgttgct ggcagtggtg tgcgtactgg
     2581 ttcagggaat gccggtatgt gcccaggaag cgcctcccgc ttcacccgat accagatttt
     2641 ttcaggccca aaagctcgaa a
//
LOCUS       DAOQDO010000726         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32790_length_2658_cov_2.988859, whole genome shotgun sequence.
ACCESSION   DAOQDO010000726 DAOQDO010000000
VERSION     DAOQDO010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32790_length_2658_cov_2.988859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1948
                     /locus_tag="PKZ53_12025"
     CDS             143..1948
                     /locus_tag="PKZ53_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNJ41211.1"
                     /translation="MKKEETPLVDRQGATKAALQLFGKYGKPYYGTFFLAFICLLLAG
                     TIALMFPYFVGRLLDSAFTLKDTRELNRTLLFLVGMLTVQSLFSFGRTYFLVYIGERL
                     VADLRRDLFDHLLKLSLSFYSNQRIGELTSRVSSDITLIQSGTTLNLAEALRQVIVFT
                     GGVTLMCVTNFRMTLTLLVIIPFLVGAAAFFGRGIRRISTRVQDQVAEASAILDEAIA
                     NIRIVQSFVREEFEVSRYKKHIESSLGHALRRALARGGFSAFIVFILFNGIVLLLWFS
                     LQQVVAGKMTLGQITSFFLYTSVVGTALGAFTELYGEFNQTLGASQRVVELFQTIPEI
                     KDAPDARQVSPVQGFVEFRDVTFAYPTRPDVTVLQNLTITANSGEIIALVGPSGAGKT
                     TVISLLPRFFDVNAGSILVDGIPVTQWKLKELRTNIGIVPQETTLFSGTIRENIAYGR
                     LDATSEEIERAAQASNAHEFIMSFPDGYETVVGERGVKLSGGQRQRIAIARALLKNPR
                     ILILDEATSSLDSESEGLVQDALETLMEGRTTFVIAHRLSTVRRADRILVLNEGRIVE
                     SGSHQELIEQNGLYQKLYTMQFRDDSPTTDVLASV"
     gene            2077..2367
                     /locus_tag="PKZ53_12030"
     CDS             2077..2367
                     /locus_tag="PKZ53_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41212.1"
                     /translation="MSSEDPTRKYPSPEDYQTRNNLEELLTRVVREVVIQEVTPQFAQ
                     LNQRLDRLEAAFQALRSDFKELRKDFRHFWAEAQRDLVELEDRIEVLETPKS"
     gene            complement(2373..>2658)
                     /locus_tag="PKZ53_12035"
     CDS             complement(2373..>2658)
                     /locus_tag="PKZ53_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41213.1"
                     /translation="RFQSVVHFPMPGHQQRLQLWEDNFKNKPYPLAADVNFSRLAREY
                     ELSGGSIINVLRYACLKAVVRNPPMIHSQDLLSGISRELHKEGKSLQHQH"
BASE COUNT          615 a          668 c          688 g          687 t
ORIGIN      
        1 gttccgggtt ccgggttccg ggttccgggt tccgggttcc gggttttcga atttaggtca
       61 ctttcgtccc ttttgtcctt agtgtccttt ttcatcattt caaaacccct gacccctatt
      121 gatattcaat ggatttttgc ctgtgaaaaa agaagaaaca ccgctggttg atcgtcaggg
      181 agctacgaag gccgcacttc agctctttgg gaaatatgga aagccctatt acggcacctt
      241 tttcctggct tttatctgtt tgctgctggc aggcacgatt gcactcatgt ttccctactt
      301 tgtcggtcgc ctgctcgact cggcatttac cctcaaagac acccgcgaac tcaaccggac
      361 gctcctgttt ctggttggca tgctgacggt gcaatcgctg ttcagctttg gtcggactta
      421 ctttctggtt tacatcggtg agcgactggt ggctgacctg cggcgtgatc tctttgatca
      481 cttgctcaaa ctgagcctca gtttttacag caaccagcga attggcgaac tgacctcccg
      541 cgtttcatct gacatcacgc tgattcaatc tgggaccacg ctcaatctgg ccgaagccct
      601 gcggcaggtg atcgtgttta ccggtggggt gacgttgatg tgtgtcacca attttcgaat
      661 gaccttgacg ctgctcgtca tcatcccgtt tctggttggc gccgccgcat tttttgggcg
      721 tgggattcgc cgaatcagca cccgcgtcca ggatcaggtc gccgaagcat ccgccatcct
      781 cgatgaagcc attgccaata tccgaatcgt gcaatccttt gtccgcgaag agtttgaggt
      841 gtcacgctat aaaaagcaca ttgagtcttc actcgggcat gcgctccgac gggcgctggc
      901 acgaggcggg tttagtgcgt ttattgtctt tattttgttt aacgggattg tgttgctgct
      961 ctggtttagt ttgcagcagg ttgtggccgg gaaaatgacc ttgggtcaga taacatcatt
     1021 ctttttgtat acatcggtgg tgggaacggc gcttggcgct tttaccgaac tctacggaga
     1081 gttcaaccag acacttgggg cttcgcaacg agtggttgag ttgttccaaa ccatcccgga
     1141 gatcaaagat gctccggatg ccagacaggt ttcgccggtg caagggtttg tcgagtttcg
     1201 cgatgtcacg tttgcgtacc caacccgccc ggacgtgacc gtattgcaaa accttacgat
     1261 taccgccaat tccggagaaa tcatcgctct ggttggaccg agcggcgctg gaaaaaccac
     1321 ggtaatttcg ctcttgcccc gattttttga tgtgaatgct ggttcgatcc tggtggatgg
     1381 aatcccggta acccaatgga aactcaaaga attgcgtacc aatatcggga ttgtgcctca
     1441 ggaaacaacc ctctttagcg ggacaattcg cgaaaatatt gcctacggac ggctcgacgc
     1501 gaccagcgaa gagattgaac gagcggctca ggcgtcaaat gcccacgaat tcatcatgag
     1561 ttttcccgat ggctatgaaa ctgtggttgg tgaacgtggc gtcaagctct ctggcgggca
     1621 acgtcaacgc atcgccattg ccagagccct gctgaagaac ccacgaattt taatcctcga
     1681 cgaagcgacc agttcgcttg attcagagtc ggaagggctg gtccaggacg cccttgaaac
     1741 cttgatggaa ggccgcacca cgtttgtgat tgcgcatcgg ctctcaaccg tgcgccgggc
     1801 agatcggatt ctggtcttaa acgaaggccg catcgtcgaa tctggctcac accaggaatt
     1861 gattgagcaa aacgggttgt accaaaagct gtacaccatg cagttccggg atgacagccc
     1921 gacaaccgat gtactggcat cagtttaagg ggttgggtca aaaaagattg agaaagaaat
     1981 tgctggccag attcgtttca gttgaggttc gacaaactca acccgatcat cagtcaaaac
     2041 cagttctaca gacagaactt tgaaaggtga ttcacgatga gcagtgaaga cccaacccgg
     2101 aaatacccgt caccagaaga ctaccaaacc cgaaataatc ttgaggaatt attgaccaga
     2161 gttgttcgag aagtggttat tcaggaagta actccccagt ttgcccaatt gaatcaacga
     2221 cttgaccgat tggaagcagc gtttcaagca ctcaggtctg attttaagga gttgagaaaa
     2281 gacttccgcc atttttgggc cgaagcacaa cgtgatctcg ttgagcttga agatcgaatt
     2341 gaagtacttg agacaccgaa gtcttgatga cctcagtgtt gatgctgtag cgatttccct
     2401 tccttatgca gctcgcggct gatgcctgac agcaaatcct ggctatgaat catcggcgga
     2461 ttccgcacca cagcttttaa acaggcgtat cgcagcacgt tgatgatgct cccgcctgaa
     2521 agttcatatt cacgggccag tcgggagaaa ttgacatcgg cggctaacgg atacggcttg
     2581 tttttgaaat tatcttccca caactgaagg cgttgttgat gcccgggcat tggaaaatga
     2641 accactgact gaaaccgg
//
LOCUS       DAOQDO010000727         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32815_length_2657_cov_2.631053, whole genome shotgun sequence.
ACCESSION   DAOQDO010000727 DAOQDO010000000
VERSION     DAOQDO010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32815_length_2657_cov_2.631053"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1651
                     /locus_tag="PKZ53_12040"
     CDS             425..1651
                     /locus_tag="PKZ53_12040"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNJ41214.1"
                     /translation="MSKANGSSSTSSQTAPTVRPAPVRISRSGFGLRTEVQDEVTSDY
                     DSPLVAQIRLQGGTLEAGRLTFRLAKEFGFCYGVDHALGLAYETHRRFPDKQLFLTGE
                     IIHNPSVNQRLQEMGFRFLKPGDPVSASDVVLIPAFGTPVGDLLKLQETGCLLVDTTC
                     GSVVHVWKRVERYAREGFTAIIHGKFYHEETEATRSQTELYPDGKFLVVRDRDEAQLV
                     CDFITCKLNREQFLAKFAPVASPDFDPDRDLQKVGLANQTTMLSSESLEIAEMIRQAM
                     MASYGVEELKDRFRSFDTICSATQERQDAVLELIEEPLDLMLVIGGYNSSNTTHLTEI
                     AVERCPTYHIDSPGCLVSAEIIRHQPVHSKNEVEASNWLPGGNLRIGVTAGASTPNRV
                     VGDCIERIISLAGEKL"
     gene            complement(1877..>2657)
                     /locus_tag="PKZ53_12045"
     CDS             complement(1877..>2657)
                     /locus_tag="PKZ53_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNJ41215.1"
                     /translation="RHNQKVVTMKDFEWAKDKVIMGSERRSMVMSNEEKRNTAYHEAG
                     HALVGMKVPNADPVHKITIIPRGMALGLTQQLPEADRYSHTREYIEGQIAILMGGRLA
                     EEVFLNHVTTGAANDLERATDLARKMVCEFGMSLLGPLTFGKKEEQIFLGREIAQHQD
                     YSEDTAIKIDQEVKRIVMDQYNRARQIILENRDALVRLAESLLEFESLDAVQVRRLIA
                     GLPLDDDSTDKKEDTRQQVDSPRPSVLKPILPPLPDNPATA"
BASE COUNT          654 a          650 c          631 g          722 t
ORIGIN      
        1 gactgcctga tcctcaaatc acgatcagtt cgcactttct taatttcacg caggtctaaa
       61 aaaccgctcg actgttttcc cactcatcgc gccgtacttc tcctccggcc aatgagtggg
      121 attttttttg aatgaagaat tgagaatgaa gaatgaagaa ttgagaccag caatagtcag
      181 tgatccgctc agattctttg attgaattac gtgaccattt tctcagccga ggattccatt
      241 acaacctgat atcagtttat ggtggtggtt gaaattcact ctaaccatca aacgacgctc
      301 agcactgcac cctgagtcag aagacgcccg ccatcccgtc ttggctttgt ttttcgccaa
      361 atgatacagt gcgcgggtcg tttgactctc tcaactccga aactccatca ggaataaagc
      421 catcatgtcg aaagcaaatg gatcaagctc tacgtcgtcg caaacagctc caaccgtccg
      481 accagcccca gttcgcattt ctcgaagtgg atttgggctt cgcaccgaag ttcaagacga
      541 agtcacgtct gattatgaca gtccgcttgt cgctcaaatt cgacttcaag gcggcacact
      601 tgaagctgga cgactgacct tccgcttggc taaggaattt gggttttgtt atggtgttga
      661 ccatgctctg ggtctggcgt atgagaccca tcgtcggttt cctgacaaac aactgttcct
      721 gaccggggaa attatccata acccatctgt aaaccagcgt ttacaggaaa tgggatttcg
      781 gtttcttaaa cccggagacc cggtttctgc cagtgatgtg gttttaattc ccgcctttgg
      841 aacgccagtt ggtgatttac ttaagctcca ggaaaccgga tgtcttttgg ttgataccac
      901 ctgcggctca gtggttcacg tctggaaacg ggttgagcgc tatgcccgcg aaggatttac
      961 agccattatc cacggtaaat tttatcacga ggaaaccgag gcgacccgat ctcaaaccga
     1021 actctatcct gatggcaagt tcctggtggt acgggatcgg gatgaagctc aactggtgtg
     1081 cgatttcatt acctgcaaac tcaaccggga acaattcttg gctaaatttg cccccgtagc
     1141 gtcacctgat tttgatccag accgggattt acagaaagtt ggactggcca atcaaaccac
     1201 catgctctca agcgaatcgc ttgaaattgc ggaaatgatt cgccaggcaa tgatggcttc
     1261 gtatggtgtc gaagagttga aagaccgatt tcgctccttt gatacaattt gcagtgccac
     1321 ccaggaacgt caggacgcag tcctcgaact gattgaagaa cctctggatt tgatgctggt
     1381 catcggtggg tataacagca gtaacacgac ccatttgact gaaattgccg ttgagcggtg
     1441 tccgacatat cacatcgaca gccctggttg tctggtctca gccgaaataa ttcgccatca
     1501 gcccgttcat agcaagaacg aagttgaagc ctcaaactgg cttccgggtg gaaatctgcg
     1561 cattggagta accgccgggg cctccacacc caatcgggtt gtgggtgatt gtattgagcg
     1621 aatcatcagt ctggccggtg agaagcttta aaccagtcag tcatcgagcg ttgggatttt
     1681 gagttacctg tccagtgtat ttggttctat gtgtgcgagt tacaaactgt ttttctcagg
     1741 aatgaattca ttgaaatatc aggattgggt ctactaatca ctgacaactg actctgccga
     1801 caaaaacaaa aaaccccaga ttcatacaga atctggggtt ttctctttga gaattttttc
     1861 tgatcaaaaa accagctcag gcagtggctg gattgtctgg caatggcggc aagattggct
     1921 ttaaaaccga tggtcgaggt gaatcaactt gctggcgggt atcttctttc ttgtcagttg
     1981 aatcatcatc gagcggcaga cctgcgatca gacggcggac ctgcacggca tcgagggatt
     2041 caaattccaa aagagattca gccaaccgaa ccagcgcatc ccgattttca aggataatct
     2101 gacgcgcccg gttgtattga tccatcacga tccgtttcac ttcctggtca atcttaattg
     2161 ctgtatcttc gctatagtct tgatgttggg caatttcacg tcccaggaaa atttgttctt
     2221 cttttttccc gaaggtcaat ggtccaagca gtgacatccc aaattcacag accattttcc
     2281 gggccagatc ggtggcacgt tcgaggtcat tggcagcccc agttgtcacg tggttgagaa
     2341 aaacttcttc agccagccgg ccacccatca aaatggcaat ctggccttca atatactctc
     2401 gggtgtgtga ataccgatca gcttctggaa gttgctgggt caatccaagt gccatgccgc
     2461 gaggaataat tgtaattttg tggactggat cggcgttggg aactttcatg ccaaccagag
     2521 catgaccagc ttcgtgataa gcggtgttgc gtttttcttc gttggacata accattgatc
     2581 ggcgctcaga ccccataatc actttgtctt ttgcccattc gaagtctttc atggtcacga
     2641 ctttctggtt atggcgg
//
LOCUS       DAOQDO010000728         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_32886_length_2654_cov_2.320123, whole genome shotgun sequence.
ACCESSION   DAOQDO010000728 DAOQDO010000000
VERSION     DAOQDO010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32886_length_2654_cov_2.320123"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKZ53_12050"
     CDS             complement(<1..1117)
                     /locus_tag="PKZ53_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ41216.1"
                     /translation="MNHQSATGSETQTESSGKNAFQKPIFLPGTGSATAILAIDYNQD
                     GVTDLIEKGGNVITIHLANSDGSFSDNKMYQLNLEEFFPTNESLPLLAAADLDDNHQT
                     LELILPVPGSFVVIFNLGFKEDTGYVFTLRPNPSTPSPSSIETTDFDNDGDIDIVLTD
                     DTANLVLVMFNNGAGIFGNGMVFETGLKPKKVAVDDFNSDGTKDLVIANVGSNSINIV
                     LGSGPTSTVQSISLPMSPDTVLVNDLNGDHNLDVMVASKGPLGKFGVLFGDGTGEFNL
                     VLSSVDLPPVSGAVIANFVGDQKPEIVFTHPESKKLTLVTFSESGMEASSLQFEGIKS
                     PISLKTNDLNQDGLPDLILSTTNKKKSIVILLNAGTSK"
     gene            complement(1434..>2654)
                     /locus_tag="PKZ53_12055"
     CDS             complement(1434..>2654)
                     /locus_tag="PKZ53_12055"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNJ41217.1"
                     /translation="ATPTASQLYFSTDSTITPTDTLVGTLNTPAIESSASVTVTGTVT
                     VPEALPAGNYFLGVMVDANRQLNETSETNNTAAVRFTVGGLADLSVPEMTVTPTLVSV
                     GDEVTVSATLQNQGMLAAPASRYVLVVSADATIGDEADAIVLTQEVASIPSNQTVQVS
                     AQVRIPAVPAGSGTLFLGLVADSENAVEESSETNNTASAQLSLKDTTPPMVRILKPNG
                     GEAIAAGRTFETIEWTASDNLAVVSQEIRLSTNGGTSFPIVILTGLSSEVRSLAWDVP
                     ADLNTTTARIQVVIQDAEGNEGQGISLADFTVARPPVIVSPPKLKKTGKLVFQATNAN
                     ILAGAQLLVTVAENTETFDLSVSSKTVLVKPTTRSVPGDRPLSQVLTKGTTATLIIRN
                     PNGVTSTPLTFTVQ"
BASE COUNT          708 a          644 c          617 g          685 t
ORIGIN      
        1 cttttgacgt accagcattg agcaggatca caattgattt cttcttattc gtggttgaca
       61 gaattaaatc cggtagccca tcctggttca aatcatttgt tttcaatgag atcggtgact
      121 taatgccttc aaactgaaga cttgacgcct ccatcccgga ctctgaaaaa gtaaccagcg
      181 tcaatttttt actctcggga tgagtaaaga caatctctgg cttttggtcg ccgacaaaat
      241 tcgcaataac cgccccgcta actggcggca gatcaactga actgagaacc aggttaaact
      301 caccagttcc atcaccaaat aaaacgccaa atttgcccag aggacctttt gacgcaacca
      361 tcacatccag gttgtggtcg ccattcaaat cgttgacaag aacggtatct ggtgacatcg
      421 gaagggaaat tgattgtacg gttgaagttg ggccacttcc caatacaata ttgatggaat
      481 tacttccgac attggcaatc accagatctt tggttccatc cgaattaaaa tcatcaaccg
      541 ctactttttt tggtttcagt ccagtctcaa agaccatgcc gttgccaaat ataccagctc
      601 cattattgaa catgaccagc accaggttgg ccgtatcatc cgtcaataca atatcaatgt
      661 ctccatcgtt atcaaaatca gttgtttcaa tagaactagg agaaggagtt gaaggatttg
      721 gacgaagggt aaaaacataa ccggtatctt ccttaaaccc taaattgaat atcacgacga
      781 aagaaccagg tacgggaagt atcagttcga gagtttgatg gttgtcgtcc aaatcagccg
      841 ctgccagtaa tggaagactt tcattggtgg ggaaaaattc ttccaggttt aactgataca
      901 tcttattgtc tgaaaaagag ccatcgctgt ttgccaggtg aattgtaatg acattccccc
      961 ctttttcaat cagatcagtc actccatcct gattataatc aatagctaag atcgccgttg
     1021 cggatccagt gcctggaaga aaaatcggct tttggaaggc attttttccg gatgattcag
     1081 tttgagtttc tgagccggta gccgactggt ggttcacgcc agaaacggat tgccattccc
     1141 ttttttgcat tgcccagcta ttggtacaaa ataaaacaac aaaacccagg cagataaaaa
     1201 ttcttacaaa atgctttatt ttttgactct ctctcaactg ttgggacata tggaaactcc
     1261 ttacaaactc aatattccga cctgccacaa aacgcgaaat atttgggttc ccatgctcac
     1321 aatcttttac tctggcaaaa tcgagagaaa atctgattgg tgaaaaaaga gtcgccaaac
     1381 agaaaggggg cattcagatg cagaattccc tctgaatgcc ccctatctga cgattactga
     1441 acagtgaagg tcaacggtgt tgaagtcaca ccatttggat ttcgaatgat aagcgtggcc
     1501 gttgtacctt tggtcagaac ctggctcaag ggccgatccc cagggacact ccgggtggtt
     1561 ggtttgacga gtacggtttt tgaactcact gaaagatcaa aggtttcagt attttcagcc
     1621 acagtgacca gaagctgagc gccagccaga atattggcat tggtggcttg aaagaccagc
     1681 ttcccggttt ttttgagttt tggcggcgaa acaatgaccg gcgggcgagc aaccgtgaag
     1741 tccgccaaac tgataccctg cccctcattg ccttcagcat cttgaatcac aacctggatc
     1801 cgagcagtgg tggtattgag atcagccgga acgtcccagg ccaggcttcg cacctcagat
     1861 gacaacccgg ttaaaatcac gattggaaac gaagtgccgc cattggttga aagcctgatt
     1921 tcttgtgata ccacagctaa attgtcactg gctgtccatt caattgtctc aaaggtccgt
     1981 ccagcggcaa tggcctctcc accatttggc ttaagaatcc tgaccattgg gggcgtggtg
     2041 tctttgagcg aaagttgggc gctggcggtg ttgttggttt cgcttgattc ctcaactgca
     2101 ttctcagaat cagcaaccag ccccagaaag agggttcctg aaccggctgg tacggctgga
     2161 atccgaactt gagcgctcac ctgaacggtc tgatttgacg gaatcgaagc cacctcctga
     2221 gtcagcacta tcgcatctgc ctcgtcgcca atggtggcgt cagccgatac aaccagcacg
     2281 tatcgagagg ccggggccgc caacatcccc tggttttgca acgtagccga aacggtcact
     2341 tcatcaccaa ctgagaccag agttggggtt accgtcatct caggcacact gaggtcggcc
     2401 agccctccga cggtaaaccg tactgccgcc gtattattgg tttcactggt ttcgttcagt
     2461 tgccgattgg catccaccat cacccctaaa aaataattgc cggcgggtaa ggcttctggg
     2521 acggtcacgg ttccggtgac agtgaccgac gctgatgatt caatcgcggg tgtattgagc
     2581 gtgccgacca gagtgtcagt cggagtaatg gttgaatcag tcgaaaaata aagttgactg
     2641 gccgttggag tggc
//
LOCUS       DAOQDO010000729         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33017_length_2648_cov_2.784034, whole genome shotgun sequence.
ACCESSION   DAOQDO010000729 DAOQDO010000000
VERSION     DAOQDO010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33017_length_2648_cov_2.784034"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..499
                     /locus_tag="PKZ53_12060"
     CDS             152..499
                     /locus_tag="PKZ53_12060"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HNJ41218.1"
                     /translation="MSSYQLDELKQQVTDLTAAEMRQLASYLLGKAEEIDQTPLSQSS
                     LQPAQKRELVMQWLTAHRSEYGGKYVALDGDRLLAVGTTYREAVEAARQSGVVNPFID
                     YVSPLDSVGFGGW"
     gene            503..919
                     /locus_tag="PKZ53_12065"
     CDS             503..919
                     /locus_tag="PKZ53_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41219.1"
                     /translation="MVHHLSFQHLTQFDPGQPGITVPIKLQVVGKEVTCDAKLDTGAT
                     YCIFARSIGEQLGLVIEAGLNQRIETVTGSFLTFGHTITLSIFDILIDATIYFADDEN
                     FSRNVLGRFGWFDRLRVGLIDYDGELYLSQYHDPIE"
     gene            complement(1028..2131)
                     /locus_tag="PKZ53_12070"
     CDS             complement(1028..2131)
                     /locus_tag="PKZ53_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ41220.1"
                     /translation="MYHSRFLCLALCCFLIASEWSVSAFAVHRSSQELPPAKSQPTDD
                     KKKVIPGQEDTVLRVRTEGALPDEGTLLRLNTQLVVVPFTVTDRQNRYINTLKAEDLR
                     LIENGQEQEVASLGRISDTPLTLALLVDFSGSMRSRLSIAKRAALQFLNQVLRLKDDK
                     AALMVFQQDVVQAAPLTNDLEVLRRGIAEADYHVPTPIGQVMPFDPGNNQPAGTALNA
                     AIYLAVDDLLSQPAASNSRRVIVLLTDGYDGEGSVQLREAIDRAWRSSVSIYAIGITS
                     PTEENAKDVNREVLERLCAATGGRAFYPRQDREFIAAFDQIEQDLRQQYVLSYTPSTR
                     DDSFRTIKIEVKDRVDLQAHHRLGYYATGDGGK"
     gene            2416..>2648
                     /locus_tag="PKZ53_12075"
     CDS             2416..>2648
                     /locus_tag="PKZ53_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ41221.1"
                     /translation="MATLNRTASPSSSRTNGKHQVILRAATKVFARNGFFNSKVADVA
                     REAGVADGTGYLYFKNKDDLLLSIINEALEIAH"
BASE COUNT          679 a          705 c          627 g          637 t
ORIGIN      
        1 atgttgacac cagtgcccgc caaatgcgaa aagccgtgga tgaagtgctt gcagaacaaa
       61 gtgtttcagg agaaattaca actcaactca attaaaaact ttacacgcga aacaaataat
      121 agagaacttg ttgaaaggaa gtgatttgat catgtcgagc tatcaactag atgaattaaa
      181 acagcaagta actgatttga cggctgccga aatgcggcaa cttgccagtt atttgcttgg
      241 aaaagcagaa gaaattgacc agacacctct atctcaaagc tccctccaac cagcacaaaa
      301 acgagaactg gttatgcagt ggctaacagc ccatcgaagt gagtacggcg gaaagtatgt
      361 cgccctggat ggtgatcggc tccttgcggt cggcacaaca taccgcgaag cggttgaagc
      421 agcacgccaa tcaggagtcg taaatccttt tattgactat gtttcaccac tagatagtgt
      481 tggtttcgga ggatggtaag ttatggtaca tcatctctcc tttcagcatt tgacacaatt
      541 tgaccctggt caacctggga tcacagtgcc aatcaaactt caggtagttg ggaaagaagt
      601 gacttgtgat gcaaaactgg acactggcgc aacttactgt atttttgcac gatctattgg
      661 ggagcaactt ggactggtaa ttgaagcagg gctcaatcaa cgaatcgaga ctgtgacagg
      721 aagtttcctc acttttggcc acaccataac actttcaatc tttgatatcc tgattgatgc
      781 aaccatttac tttgcagatg atgaaaactt ctctcgaaat gtacttgggc gatttgggtg
      841 gttcgaccgg ttgcgcgttg gcctgattga ctacgatggt gaactttatc tcagccaata
      901 tcacgaccca atcgaataac aattttaaac ttcttgatca atccctcttt tctttcctta
      961 caccttcttc ttcagccccg agcttgcgag tcttcagccc aatttcttca gccccaggcc
     1021 ctcagcccta tttccctcca tcccccgtgg cgtaataccc aagccggtga tgcgcctgta
     1081 aatcaacgcg gtctttgact tcgattttga tagtgcggaa ggaatcatca cgcgtggatg
     1141 gcgtgtagga aagcacgtac tgttgccgca aatcctgctc gatttggtcg aaggcagcaa
     1201 tgaactcccg gtcctggcgt ggataaaacg cccgtccacc agttgccgca cagagccgtt
     1261 ccagcacttc gcggttgaca tccttggcat tttcttccgt cgggctggta atcccaattg
     1321 cataaatgct cacgctcgac cgccaggccc ggtcaatcgc ttcgcggagc tgaacgctcc
     1381 cttcgccatc atacccatcc gtcaggagca caatcacccg ccgtgaattg gacgccgccg
     1441 gttgtgacaa caaatcatcc accgccagat aaatcgccgc gttgagcgct gttcctgccg
     1501 gttggttgtt gcctggatca aacggcatca cctgcccaat cggagtcggg acgtggtaat
     1561 cagcttcggc aattccccgc cggagcactt ccagatcatt ggtcaacggc gccgcctgaa
     1621 ccacatcctg ctgaaaaacc atcaaggccg ccttgtcgtc ctttaaccgc agcacctgat
     1681 ttaaaaactg aagcgctgcg cgtttggcaa tcgaaagccg gctccgcatg ctgccgctaa
     1741 aatccaccag caacgccagc gtcagcgggg tgtcagagat tctccccagc gaagcaactt
     1801 cctgctcctg gccattttca atcagccgta agtcttccgc tttcaatgtg ttgatatagc
     1861 gattttgccg gtcggtcacc gtaaacggaa caaccacaag ctgggtgttg agccgcagca
     1921 gggtgccttc gtccgggagc gcgccttcgg ttcgcacccg cagaacggtg tcttcctggc
     1981 cgggaatgac cttctttttg tcatcggttg gctgcgattt ggcagggggt aattcctgag
     2041 aggaacgatg aaccgcaaaa gcagagacac tccactcact tgcgatgaga aagcagcaca
     2101 acgccagaca caaaaatcgg gaatggtaca taacaaggtt cacaacttgg aaaatgaagc
     2161 tgcctgtgca gcaggttgga tgcagagtaa aagattcctt ttgaaaagaa aagcgggtga
     2221 aatttcggaa gcagggtctt gcgcactgcg tcaatcttca gtaagatgaa tgacgattca
     2281 ttcagtctcc aaaacaatca aacaagtgct taccgtgtgt gtcaaccagg cagcgaacgg
     2341 cttcatccct cctccctcga accggcgctt cctcgcttcc aaacccttac ttcctcaaga
     2401 tctttgcgca tggctatggc cacattgaac cgaactgctt cgccttcttc cagccgtacg
     2461 aacggaaaac atcaggtcat cttacgggcg gccaccaaag tctttgcccg caacggtttt
     2521 ttcaattcaa aagtggcaga tgtggctcgt gaggccggag tggccgacgg aacggggtat
     2581 ctgtacttca aaaacaaaga tgacttgctg ctttcgatca tcaacgaagc ccttgaaatt
     2641 gctcacga
//
LOCUS       DAOQDO010000730         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33042_length_2647_cov_3.282793, whole genome shotgun sequence.
ACCESSION   DAOQDO010000730 DAOQDO010000000
VERSION     DAOQDO010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33042_length_2647_cov_3.282793"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKZ53_12080"
     CDS             complement(<1..396)
                     /locus_tag="PKZ53_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNJ41222.1"
                     /translation="MVESDLSPRETLKSLGWHWLKFNAVGAVGMALQTLMLEILSHWF
                     GLRSSVAMVIAVELAVIHNFFWHEKWTWSDRPSLTWKDRLLRCVNFNLTNGLVSILGA
                     IVFMELLHERLHLPVQLVNLISIIACSLIN"
     gene            complement(396..1070)
                     /locus_tag="PKZ53_12085"
     CDS             complement(396..1070)
                     /locus_tag="PKZ53_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNJ41223.1"
                     /translation="MEKSDFKDAKRQLAGLTAPLERRAIQFCVQKLPAWVNSDHLSLL
                     GFLALVGAGALYSISKSNPVLYLTLVNVCLLLNWFGDSLDGSLARYRQKQRPRYGFYV
                     DHIIDTIGFFFLIGGMAVSGYMSERVAFGLLTVYYMLMINSNLSAYTLSKFNVSYGLF
                     GPTELRIVLAIGNFFLITHPFSTILGHKYLLYDVGGVCSIIGMGITFLIAVLKNIVTL
                     YNLERV"
     gene            1293..2570
                     /locus_tag="PKZ53_12090"
     CDS             1293..2570
                     /locus_tag="PKZ53_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41224.1"
                     /translation="MKLLSLSLFVVLLFSTAVVAQNSPTASQSGAPASTTEATSSPTG
                     STLAEFDRLRLEGFTAVYNIDYATAKKDFRQMIELDPEKPAGYLYVANALWLEQLNKS
                     RRLQIGVYNDDSFYSETKETIDPKFDKEFRDLLAKAIAKAEARLKTTPNDTQTLYFLG
                     AAHGVLAAYEATVARSFISALKNGDKSVDLHRKVLELDPKCMDANLTIGMYNYVVGSL
                     PLPVKVLAAMGGFRGSKKKGLALLEQVTEQGTYVPDDARVILTALYKREKRYADALRQ
                     LEVLGKKYPANYLVKMETADALIRLGKYDESSKLFAELLADPRTATSRDLIEYQFAEA
                     LAAQDRDADAAEHYAAVIAFKNANADLVTLSHLKLGQINDLLSKRQEAVAHYNKVLSR
                     KNVFDSQDRAKEWLKKPYVRSRNEAKPDTPAEP"
BASE COUNT          743 a          658 c          634 g          612 t
ORIGIN      
        1 attgatcaat gagcaggcga taattgagat caaattaacc agttggacag ggagatgcag
       61 tctttcgtgt agcaactcca taaacacaat tgcccccagg atggatacca atccattggt
      121 caagttgaag ttgacacagc ggagcaggcg atccttccag gtcaggcttg gtcggtcgct
      181 ccaggtccat ttctcatgcc agaagaagtt gtgaatcact gccagttcaa cggcgatgac
      241 catggcgacc gatgaccgca ggccaaacca gtgggacaat atttcaagca tcagcgtttg
      301 gagcgccatt ccgaccgcgc cgacggcatt gaattttaac cagtgccagc caagtgactt
      361 cagggtttca cggggagaca ggtcactttc aaccatcaca cacgctccag attatagagg
      421 gtcacgatat ttttgagcac tgcgatcagg aatgtgatcc ccatgccgat gatgctgcat
      481 accccaccca catcatagag caggtatttg tggcccaaaa tggttgagaa gggatgtgtg
      541 atcaagaaga aatttccaat ggcgagcaca atccgcagtt cggtcgggcc aaataaccca
      601 taggaaacat tgaatttact taaagtatat gctgaaaggt tcgagttgat catcagcatg
      661 tagtagaccg taagcaaccc aaaagcgacg cgttcgctca tataccccga aactgccatt
      721 ccgccaatta agaagaaaaa accaatcgtg tcgatgatgt gatcaacata aaacccatat
      781 cgtggacgtt gtttttgccg gtatcgagcc agtgatccgt ccaaactgtc accaaaccag
      841 ttgagtaaca ggcagacgtt gaccagggtg agatagagca ccgggtttga tttactgatc
      901 gaatacaggg cgccggcacc gaccagggcc agaaacccca gcaacgacag gtgatcagaa
      961 ttaacccagg ctggcaattt ttgtacacaa aactgaattg cccggcgttc aagtggtgcg
     1021 gttaaccccg caagctgacg tttcgcatct ttgaagtctg atttttccat gatttttctt
     1081 gggtgaaact ggattgagac caatgatgag gagtgaatga tgaatgacca gagagctaac
     1141 tatctgtttt tcaggcagat ggcattgtca caactcatta tgcctcatgc atcattcgtg
     1201 ttgaaaaccc aggtctgatg tcatcaacca gaacacggtt gtccttttct acaattccac
     1261 attgctttga ccaaaaggcg atttcaaaca ttatgaaact cttgtcactg agcttgtttg
     1321 tggtcctcct gttttcaacg gctgttgtcg cacaaaactc acctactgca tctcaatctg
     1381 gcgcgccagc atccacgact gaagccacgt cgtcacccac tgggtcaacc ctggctgaat
     1441 ttgaccgact ccggctggaa ggctttactg cggtgtacaa cattgactat gccaccgcga
     1501 aaaaagactt ccgccagatg atcgaacttg acccggaaaa acccgccggc tatttgtatg
     1561 tggccaatgc cctctggctg gaacaactca acaaaagccg tcgcctgcaa atcggtgttt
     1621 ataatgacga ttcgttttat tcagaaacca aggaaaccat agaccctaaa tttgataaag
     1681 aatttcgcga cctgctggcc aaagccattg ccaaagctga agcccggctc aaaacaacac
     1741 caaacgacac gcagacgttg tatttccttg gtgctgccca tggtgtcctt gcggcatatg
     1801 aagccaccgt cgcccggtca tttatttcag ccctcaaaaa cggagataaa tccgtagacc
     1861 tgcatcgcaa agtcctcgaa ctggacccga agtgtatgga tgccaatttg accattggca
     1921 tgtacaacta tgtcgttggg tcgctcccac tgccggtcaa agtgctggcg gcgatgggag
     1981 ggtttcgcgg ttcaaagaaa aaagggctgg ccttgctcga acaggtcact gagcagggaa
     2041 catatgtccc ggatgatgcc cgcgtgattt taactgcatt gtataagcgc gaaaaacgct
     2101 atgcggacgc gctccggcaa cttgaagtcc ttgggaagaa atacccagcg aactatctgg
     2161 tcaaaatgga gaccgccgat gctctgattc gacttggaaa gtatgatgaa agttcgaaat
     2221 tgtttgccga attactggct gatcctcgaa ctgccacctc acgcgattta atcgagtacc
     2281 agtttgccga agccttagcc gcacaggatc gcgatgccga tgcggctgaa cactatgctg
     2341 ccgtgattgc tttcaaaaat gccaacgctg acctggtgac gctctctcat ctcaagcttg
     2401 gtcagatcaa tgatcttttg agcaagcgcc aggaggcggt ggctcattat aataaagtgt
     2461 tatcgcgtaa aaacgttttt gactcgcaag atcgagccaa agaatggttg aaaaaaccct
     2521 atgttcgctc ccggaatgaa gccaaaccgg acaccccagc cgaaccctag ccgaaccctg
     2581 gccgaaccct ggccgaatga agaatgaaga atgaagaatg aagaatgaag aatgaagaat
     2641 gaagaat
//
LOCUS       DAOQDO010000731         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33100_length_2644_cov_3.724218, whole genome shotgun sequence.
ACCESSION   DAOQDO010000731 DAOQDO010000000
VERSION     DAOQDO010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33100_length_2644_cov_3.724218"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKZ53_12095"
     CDS             complement(<1..928)
                     /locus_tag="PKZ53_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ41225.1"
                     /translation="MPALLSLQDLKVHFPVRSPGLKSVFSRQKARVVHAVDGVTFEIQ
                     GGETLGLVGESGCGKSTTGRAILRLVEATGGKVLFQGQDVCSLGASQLRQTRRHMQMV
                     FQNPYASLNPRMTVGDIIGEPLKTFGQGGKDLAVQVQTLMQTVGLNPGVVRQYPHEFS
                     GGQRQRIGIARALALNPQLVVADEPIAALDVSIQAQILNLLKRLQQERKLTYLFISHD
                     LRAVRHISNQIAVMYLGKIVELGDAKTVYTQPQHPYTQALISAVPVPHPKIEKVRQRI
                     VLQGDVPSPINPPTGCRFHTRCPHVMPMCREAE"
     gene            complement(1063..1956)
                     /locus_tag="PKZ53_12100"
     CDS             complement(1063..1956)
                     /locus_tag="PKZ53_12100"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ41226.1"
                     /translation="MPTTPPSIFILGITQRSGTNYLHDLLALHPDCRPGGVIFEDFLI
                     HYSDPLARYVSTIYDHWSPEWNVLEKIGPEEVLFECLGNGLLEFLARQWKSASTELNP
                     RQRLLSKTPSVVNLRHFFRFFPHAHLLIVVRDGRAIAESAHRSFNTAYERSFQELAAG
                     AREILRFKRVTNQETHPFSIVRYEDLYPDPEPRLRELFPFLGLDPQTFDFDKARNLPV
                     RGSSDVKQTEGQLHWKPVEKKADFNPLNRFAHWPPALHERFVWVVGPYLAELGYESAQ
                     VMVPGFLKKLRHRWLDWRWKE"
     gene            complement(2331..>2644)
                     /locus_tag="PKZ53_12105"
     CDS             complement(2331..>2644)
                     /locus_tag="PKZ53_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41227.1"
                     /translation="LVTLSACETGVGEAKTGDGVYGLRRALVLAGSEAQLMSLWSVSD
                     QATRKLMVDYYTRLKAGEGRSEALRNVQLEMLKDPRRQHPFYWASFIQSGEWANLDGK
                     R"
BASE COUNT          654 a          676 c          674 g          640 t
ORIGIN      
        1 gttcggcttc gcggcacatt ggcatcacgt gcgggcagcg agtatgaaac cggcaccctg
       61 tcggagggtt gatcgggctc ggaacatcgc cctggagcac aatccgttgc cggacttttt
      121 caattttggg atgaggtacc ggcacggctg aaatcagcgc ctgggtgtag ggatgttgcg
      181 gctgggtata cacggttttg gcgtctccaa gctcgacaat ttttcccaga tacatcacgg
      241 caatctggtt gctgatatgc cggacagctc gcaagtcgtg tgaaataaac aaataggtta
      301 attttcgttc ctgttgtaat cgcttcagga ggttgagaat ctgcgcctgg atcgaaacat
      361 caagtgccgc aattggctca tcagccacca ccagttgcgg attgagtgcc agtgcccggg
      421 caatgccaat tcgctgccgc tgaccacctg aaaattcgtg cgggtactgc cgcacaaccc
      481 caggattgag cccaaccgtt tgcatcaggg tttgtacctg cactgccaga tcttttccgc
      541 cttgcccaaa tgttttcaag ggttcgccaa tgatgtcacc cacggtcatt cgcggattga
      601 gcgacgcata gggattctga aaaaccattt gcatgtgccg tcgtgtctgg cgtaactgac
      661 tcgcacccaa cgaacacaca tcctgccctt gaaagagaac ctttccacca gtcgcctcaa
      721 ccagtcgtaa aatggcacgt ccggtggttg attttccaca cccggattca ccaaccagcc
      781 caagcgtttc accaccctga atttcaaacg tcacaccatc aacggcgtgc acaacacgtg
      841 ctttttggcg gctgaacaca ctcttgagcc caggcgaacg cacgggaaaa tgaactttta
      901 aatcctgaag cgagagaaga gcaggcatag caaagtagtg aagtagtgaa gtagtgaagt
      961 agtgaagtag tgaagtagtg aagtagtgaa gctttgactg ttagatcaat tctgtcaact
     1021 tcttaatttc cctggttttg actatttcgt tatttcgctc tttcactctt tccatcgcca
     1081 atccagccag cggtggcgga gttttttcag gaagccaggt accatcactt gagctgactc
     1141 atacccaagt tcagccaaat atggcccgac aacccagaca aatcgctcgt gcagcgcagg
     1201 cggccagtgg gcaaaccggt ttaacgggtt aaaatctgct ttcttttcaa ctggtttcca
     1261 gtgcaactgg ccttcggttt gtttcacatc cgatgagcca cgaaccggca ggtttcgcgc
     1321 tttgtcaaaa tcaaaggttt gtggatccaa tcccagaaaa ggaaagagtt cgcggagcct
     1381 gggttctgga tcaggataga gatcttcgta acggacaatt gaaaaaggat gggtttcctg
     1441 atttgtaact cgtttgaatc ggagaatttc ccgtgcgccg gctgccagtt cctgaaatga
     1501 ccgttcatac gcggtattga aactgcgatg ggctgactca gcaattgccc gtccatcacg
     1561 caccacaatc agaagatgag cgtgcggaaa aaatcggaaa aagtggcgca aattgactac
     1621 gcttggggtt tttgaaagca gccgttggcg tggatttagt tcagttgacg ctgatttcca
     1681 ctggcgggca agaaactcaa gcaacccatt tccaaggcat tcaaacaaca cttcttccgg
     1741 gccgattttt tcaaggacgt tccattccgg actccagtgg tcatagatag ttgagacata
     1801 tctggccagt gggtcagaat aatgaatgag aaaatcttca aaaatcacgc cacccggacg
     1861 gcaatcaggg tgtagcgcca gcaggtcatg caggtaattg gtgccgctac gctgggtaat
     1921 tccgaggata aaaatcgatg ggggagtggt gggcatacgt cagggctgaa aacttcgggc
     1981 ggaagaatta gttttatttc atccctgagg gcgggaaaat tttgaaacca taataaacca
     2041 tcacacctga ttattaagga ggttcctgct tctaccggat tttgtgtttc gccaggtcgg
     2101 tgttgaaaat aaagggctgg tcagtaccac ctgcgtgagc gggtggggtt ggaagccagg
     2161 agacccaccc actgacgcgg gtggtactga cacatcggtt cttctgatcc gcctccaaca
     2221 caaaatccgg tagaagcaga atcactcaca gtagagaaca gtcgagtaat tcaatcaaat
     2281 gggcttagcg aactactgac tactgactac tgactacaaa ctggtgtttg ctatcgtttc
     2341 ccgtccagat tggcccattc gcccgattga ataaagcttg cccagtagaa tggatgctgc
     2401 cgcctgggat ctttgagcat ttccaactgc acattacgca aggcttccga gcggccttca
     2461 cccgctttga gtcgggtgta gtagtccacc atcaatttcc gcgttgcctg gtccgacacc
     2521 gaccacaggc tcatcagttg ggcttcgctt ccggccaaaa ccagtgcccg ccgcaaccca
     2581 tacaccccat ccccggtttt ggcttcgccg actccggttt cacaagccga gagggtgacc
     2641 agct
//
LOCUS       DAOQDO010000732         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33113_length_2644_cov_2.761684, whole genome shotgun sequence.
ACCESSION   DAOQDO010000732 DAOQDO010000000
VERSION     DAOQDO010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33113_length_2644_cov_2.761684"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKZ53_12110"
     CDS             <1..271
                     /locus_tag="PKZ53_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41228.1"
                     /translation="NLTVVDETPDQHLIARLYSIERQHQSAELASVELPFTPDRAFAV
                     ELDVSELEEDSYILKVFDADDAQAVPVERFVFTLLFEERDHSLKG"
     gene            complement(442..1824)
                     /gene="fliD"
                     /locus_tag="PKZ53_12115"
     CDS             complement(442..1824)
                     /gene="fliD"
                     /locus_tag="PKZ53_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNJ41229.1"
                     /translation="MSGITNLSGVGSGIDFGVITDSLVAIRSRPITQLSSTQSGYRGR
                     ADVLKQLNTRLITLKEAIGGLTDRTLSTGRFTASSTPTVTTATAATTASLGTVQVEVL
                     RLASQFSQATAGFPTTETAILANGATTATFELRKGGATTGTPLTITTANNSLAGLRDA
                     INSAKAGVSASIVDVAGNGTQNQLVLTSTETGAAGSVELVETTSTGTGTALNLRSVGL
                     AGANLDASLKINGLEISRPGNTISDALTGVSLSLKSTGTTTISITSNTSTLKSKIENF
                     VTAYNAAQDLMNDQYKPDSKGRASGILAGNPTLRQVQYELRNLLQTNATGNGGNLTGL
                     SQIGLGRDATGKITIDQETLNTKLTDSLADVQALFTGKTETQTGLAQFFETSVSPIVS
                     TIEQAITGFQSSIQQLDKNIADQQTRLLTYRKALEHRFSVVDAAIGQINGQSAALTSV
                     IKSLDSSKTD"
     gene            complement(1906..2280)
                     /locus_tag="PKZ53_12120"
     CDS             complement(1906..2280)
                     /locus_tag="PKZ53_12120"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNJ41230.1"
                     /translation="MYPEFATLPGLFRLEPSEKIHGTGLRNSQPAEPISAGQKTGIET
                     DVPVPGIQKGPASAHQTITEITDALNISLRFRRDEATHQIVVELMDRNSGELIRQVPS
                     ETMLRLSTALSELQGPFINLAG"
     gene            complement(2385..>2644)
                     /locus_tag="PKZ53_12125"
     CDS             complement(2385..>2644)
                     /locus_tag="PKZ53_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNJ41231.1"
                     /translation="AIKNAISTLGTIQGKVGAGQNNLTQAIDLATSQLSNYQAAESAI
                     RDSDIASEASNLARLTTLQQAGVSALAQANQSNQALLSLLR"
BASE COUNT          547 a          656 c          730 g          711 t
ORIGIN      
        1 caatttgacg gttgtggatg aaacgccaga ccagcatttg attgcccggc tctatagtat
       61 cgagcgccag caccagagtg ccgagctggc ttcggttgag ttgccattta caccagatcg
      121 ggcatttgcc gtcgaactgg atgtgtcaga actggaagaa gattcttata ttctgaaggt
      181 ctttgacgcc gatgatgctc aggcggtacc tgttgaacgc tttgttttta ccctgctgtt
      241 tgaagaacgt gaccattctt taaagggttg acgacgtcgg gtttggggct tggggctaag
      301 ggctaagggc tgggagttga ggaattggtt ttatttcatc cctcatttct catcccttcg
      361 attgctccga gcttgcgagc cctcagccca gtttcttcag cccccagccc gttttcttca
      421 gccctcagcc caattctttc atcagtcggt tttgctggaa tcaagcgact taataaccga
      481 cgtcaaagct gcggattggc cgttgatctg gccgatagcg gcatccacaa ccgaaaaacg
      541 atgttcaagc gctttgcgat aggtcaacag ccgggtttgt tgatcggcaa tatttttatc
      601 aagttgctgg attgaggatt gaaacccagt gattgcctgt tcgatggtac tgacaattgg
      661 gctgactgag gtttcgaaaa attgtgccag tccagtttga gtctcggttt ttccggtaaa
      721 taaagcctgc acatcagcca gtgagtcagt cagttttgtg ttgagtgttt cctgatcaat
      781 ggtgattttt ccagttgcat cgcggcccag tccaatttgt gacagccctg tcaggttccc
      841 gccattcccg gttgcgtttg tttgaagcag gttgcgaagt tcgtattgaa cctgccgcaa
      901 cgttgggttt ccagccagta tcccactggc tctccctttt gaatccggct tgtactggtc
      961 attcatcagg tcctgcgccg cattgtacgc cgtcacaaaa ttctcaattt tacttttgag
     1021 ggtgctggta ttgctggtaa tgctaatggt ggtggtcccg gtgcttttca atgacaggga
     1081 aactccagtc agagcatcgg aaatcgtatt gccagggcgg gaaatctcca ggccgttaat
     1141 tttgagtgag gcatccaggt tggctccagc cagaccgacg gaacgcaggt tgagcgccgt
     1201 gccagtgcca gtactcgtcg tttcaaccag ttccacgctc cccgccgccc cggtctcggt
     1261 tgaggtcaac accagttgat tttgagttcc attgccggcg acatccacaa ttgaggctga
     1321 gaccccggct ttggcgctgt tgatggcgtc gcgtaatcca gccagggaat tattcgccgt
     1381 ggtaatcgtc agcggtgtgc cggtcgtggc cccgcctttg cgcagttcaa acgtggcagt
     1441 ggtggcgcca ttggccaaaa ttgccgtttc tgtagttgga aacccggcgg tggcctgtga
     1501 aaactggctt gccaggcgga gaacttcaac ctggacggtg cccagtgacg cggtcgtggc
     1561 cgccgtggct gtggtaacgg taggggtgga cgacgccgta aagcgtccag tgctcagtgt
     1621 gcggtcagtt aagcctccga tggcttcttt gagcgtaatc agccgcgtat tcaattgctt
     1681 gagcacatca gcccgtccgc gatacccgga ctgggtactc gataattgag taattggtcg
     1741 gcttcggatg gccaccagtg aatcggtaat cacaccaaaa tcaatcccgc ttcctactcc
     1801 gctgagattt gttattccag acatacatac ctcaattggt ccacatcagt tggcagtcat
     1861 ttcaagtggc ttgcccgacc ctgcttgcgg tgattgatga gaaccctatc cagccaggtt
     1921 gataaatggc ccttgcagtt cgctcagcgc agtcgaaagc cgtagcatcg tttcactcgg
     1981 aacctgtcga atcaactcac ctgagtttcg atccatcagc tcaacgacaa tctggtgggt
     2041 ggcttcatcg cgtcgaaacc gaagcgaaat attaagggca tcagtaattt cagtgatggt
     2101 ttggtgtgcc gacgcgggtc ctttttgaat tccgggtacc gggacgtccg tctcgatgcc
     2161 tgttttttgg cccgcgctga ttggttcggc aggttgtgaa tttcggagac cggtgccatg
     2221 gattttttcc gatggctcaa gtcgaaagag cccagggagc gttgcaaatt cggggtacat
     2281 acttcactcc attgaaacaa gaatcaattc cagctttgat tcgcgccctg ctgatgcgaa
     2341 acaaaactgg aattgttctt cggagaaaag tcaggcgaaa ggacttaccg cagcagcgag
     2401 agcagagctt gatttgattg gtttgcctgc gctaaggccg aaacaccagc ttgttgcaag
     2461 gttgtgagtc gggccaggtt cgaagcttca gaggcaatgt ctgaatcgcg gatggctgat
     2521 tcagccgcct gatagttgga cagttgtgag gtggccagat caatggcctg ggtcaggttg
     2581 ttttggccag ccccgacttt gccctgaatc gttcccaggg tcgaaatggc gttcttaatg
     2641 gctt
//
LOCUS       DAOQDO010000733         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33257_length_2638_cov_2.907472, whole genome shotgun sequence.
ACCESSION   DAOQDO010000733 DAOQDO010000000
VERSION     DAOQDO010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33257_length_2638_cov_2.907472"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKZ53_12130"
     CDS             complement(<1..1061)
                     /locus_tag="PKZ53_12130"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNJ41232.1"
                     /translation="MMLVLGTGEQVLAQGINYPVSNPAGTSGTGNARTDPDNLFIRNN
                     IAGMTEIAVNDTEEADGKLGESGKGGWRFLGELQGAYFRYERERNAGPFQGVKSNATI
                     YPPGLAGEITYTSGNHKYAFGVGVYSVFAFQSKVVDPATLGPRALFFDSKVGSTDVAF
                     GGAARLNKYVSVGGSFIFGRGFVDLKAPNSALLAAGILQDSRVDVTDIGAPGFSVGVN
                     LRPNDKINFGFNYKSRREYDMTGVLTTAIPRPVSGGIQLVPFKADITTQFKLPQVVES
                     GIQFKATKNFTFVADYRFYDYPNALETVVVKSEQSGQPLLSQTINAKNVHSVRFGSIY
                     TVNDTTRLNFGLAFTSDGFR"
     gene            1544..2317
                     /locus_tag="PKZ53_12135"
     CDS             1544..2317
                     /locus_tag="PKZ53_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HNJ41233.1"
                     /translation="MKFDELDTKMRIFETAHDHCVLPGLFMVARLDGRNFTRLTREVC
                     NFEAPFDVRFRDMMTSTVEHLMQCGFRVVYGYTQSDEISLLFHREIDVFQRKLRKYNS
                     VLAGEASAKFSILLGQIGCFDCRISQFPGLEQVYDYFLWRSEDAHRNALNGHCYWLLR
                     KEGETPEAAANVLFGMSVPEKNEFLFDRGVNFNEIPAWQKRGAGVYWETYYKQGLNPI
                     TGESVQAERHRLKVELDLPWRDNYVRLLQQLIVRSEVAK"
     gene            complement(2389..>2638)
                     /locus_tag="PKZ53_12140"
     CDS             complement(2389..>2638)
                     /locus_tag="PKZ53_12140"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNJ41234.1"
                     /translation="YDPGVDTVYLGSALLTLFLWMVFFFSHQRLWILIESRPDGTSVI
                     SITGNTNRNRPFFERTFTRLANYLTGKSTTLDPQPEAE"
BASE COUNT          680 a          636 c          678 g          644 t
ORIGIN      
        1 cggaatccat ctgaggtaaa cgccaaccca aaattcaggc gcgtggtatc gttgacggtg
       61 tagattgagc caaaacgcac ggaatgcaca ttcttggcat tgatcgtctg tgacagcagt
      121 ggctgtccac tctgctcact tttcaccacg acggtttcca atgcgttcgg gtagtcatag
      181 aaccgatagt cagccacgaa ggtgaagttt ttcgtggctt tgaactggat accgctttca
      241 acaacctgcg gcagtttaaa ctgggtggtg atgtctgctt tgaatggaac cagttgtata
      301 ccaccgctaa caggtcgcgg aatcgccgtc gtcaagacac ccgtcatgtc atattcgcgt
      361 ctgctcttgt agttgaaccc aaaattgatc ttgtcatttg ggcgaagatt cacgccgaca
      421 ctaaacccag gggcgccgat atcagtcaca tcaacccggc tgtcctgcaa aattccagcc
      481 gctaacaacg cggagttggg tgctttgagg tcaacaaacc cacgtccaaa gataaaacta
      541 ccaccgacag agacgtattt gttgagtcgg gcggcaccac caaaggccac atcggttgag
      601 ccaaccttgg aatcaaaaaa cagagcacgt ggtcccaggg tagctgggtc tacgactttg
      661 ctttggaagg caaatactga atacactcca accccaaacg catatttgtg gtttccactg
      721 gtataggtga tttcaccggc cagacctggg gggtagatgg tcgcgttgct cttgacgccc
      781 tggaacggcc cggcgttgcg ctcgcgctca tatcgaaaat aggcaccttg taattcaccc
      841 agaaaccgcc atccaccttt gccgctctcg ccaagttttc catcagcttc ttcggtgtca
      901 ttgactgcaa tttcggtcat acccgcaatg ttattgcgga taaaaagatt atccgggtcg
      961 gttcgggcat ttcccgtgcc gctcgtcccg gctggattac tcaccgggta gttaatacct
     1021 tgagccagga cctgctcacc tgtacccagg acgagcatca tgcctaaccc tgcgaacaag
     1081 accaaaagct tgatcttgtt atgcaacaga aggggacaaa aagttgaatt catttttttt
     1141 ctccacggaa tgcaacacaa ggatgatgaa gaactcagtt cctgggaaaa aggggacaga
     1201 caagcaggcg ggtgtgtaga aatcccctga caggaacaga acattgatac cagagcaatg
     1261 cgcattccac aaattttcta ttcaatccat ttcaaaggat gtgtcagtgc tgccattttg
     1321 ccatcaagtc gccggattat ggacgtgtca gtaccacctg cgtgagcggg tgggagtgga
     1381 aaccagccga cccacccact cacgcgggtg gtactgacca agacgtatat tgacgataac
     1441 cctgggaatt ggcgcagttc agccacacaa gagagtacac tttgaagatt gagccctgat
     1501 tgaataagga tatgaaaatc gaaaaggtga ggaatttaca ccaatgaaat ttgatgaact
     1561 ggataccaaa atgcggattt ttgaaactgc ccacgatcat tgtgttttgc cagggctgtt
     1621 tatggttgca cggctggatg ggcggaattt tacgcgtctg acccgcgaag tgtgtaactt
     1681 tgaagccccg tttgatgtgc gttttcgcga tatgatgact tccacggttg agcacttgat
     1741 gcagtgtggg tttcgcgttg tgtatggata tacacaaagt gacgaaatct cattgctgtt
     1801 tcatcgcgag attgatgtgt ttcaacgcaa actccgcaag tacaactcag ttcttgccgg
     1861 agaagccagt gctaaatttt caattttgct ggggcaaatc ggatgttttg attgccgaat
     1921 ctctcaattt ccagggcttg agcaggtcta tgactatttt ctgtggcgga gcgaagatgc
     1981 ccatcgcaat gccctcaacg gacattgcta ctggctattg cgcaaagagg gcgagacccc
     2041 ggaagcggct gccaacgttt tgtttggaat gtcagtcccg gaaaagaacg aatttctctt
     2101 tgaccgtggg gtgaatttca atgagattcc cgcctggcaa aaacgtggtg ccggggtgta
     2161 ttgggagacc tactacaaac aaggcttgaa tccaatcact ggtgaatcgg tccaggccga
     2221 gcgccaccga ctcaaagtcg aacttgatct gccgtggaga gacaattatg tcaggctgtt
     2281 gcaacagttg attgtcagga gcgaagtcgc gaaatagcga aatagcgagt cgtcaatcca
     2341 gaaagcctgt gagtcaagtt atcatcttgt taccgtgtca cttaatcatc actcagcttc
     2401 gggttgtggg tccagtgtcg tggatttacc tgtcaggtaa ttggccagtc gggtgaaggt
     2461 gcgctcaaag aacggtcggt tgcgattggt attcccggta atggaaatca cgctggtccc
     2521 atctgggcgt gattcaatca aaatccacag gcgctggtgc gagaaaaaaa acaccatcca
     2581 cagaaacaac gtcagtagcg ccgagccaag gtagacggtg tctacgccag ggtcatac
//
LOCUS       DAOQDO010000734         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33341_length_2634_cov_2.964327, whole genome shotgun sequence.
ACCESSION   DAOQDO010000734 DAOQDO010000000
VERSION     DAOQDO010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33341_length_2634_cov_2.964327"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2634
                     /locus_tag="PKZ53_12145"
     CDS             <1..>2634
                     /locus_tag="PKZ53_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41235.1"
                     /translation="VFRLSSELFASYNTTGLTRGMVTGVGEASDGTLWVASMTTGLSS
                     LSPSGEMRRYTSKDGILEENNLWCLLVDRRDRVWVGGYQGVSCFDGQRFVQFPREKMG
                     AQDRITAIGEDRQGHIWFGSATSTSNGVVRYDGQTFQRFTDQDGLSSNQVSGIRLDQK
                     GDIWFCTENGLTRYDGQRFQAFTQKDGLPSRRVRTLYQDHDGTYWVGTTDGLCRFDGT
                     RVTQIFKEKEGLPGSLIRTIQRFDKTLWIGTQRGIAEFDGQQVVRVYSVRDGLVNDEI
                     INNACLQRRDGSVWFGTTEGVVRYRVAQEIAHPMPPKVHLTGIRVTGAGAPNLVAPGN
                     NQSAPLPTLDYDQNSLTFEFLGLTFLDETAVRYTYRLEPFDREWSAPSPERTARFTNL
                     PPGRYRFAIKACSVRETWSEPQYFEITIRPPFWQTWWFRALSVAALITLISALYTWRI
                     HALNQRQQQKLEHLRQLQEQRMMVMRQLLESIQVINSQLETTAVLQNIAEESARLVDG
                     KPGGIGLVQDDRVVFKRQWVNGSWQAGDVSFQFGEGTPGKVARSAKAIIVNDPDDEPV
                     SFVPEQARKNTSDGWMDVPILTRAGEVAAVLDIRHRPGRPPFTNEDISLIESLASQAA
                     VAIENASLYGELEEKNLMIAESFRELERLYQQEQEVSRTLQELNQMKTNFIVVTSHEM
                     RTPLTVIRGYNEALLGEFFGELNPMQHKSLSVTQRMIDRMVNSFNDILEMLKINEGQF
                     TLKREPANLKDLVQAVLEELQPFVEKRKQTLTIVGPDVMATVDREKFGLVLLNVVQNA
                     IKFTQDTGTIQISLGREGESAHIRVIDSGIGIDASELERIFDKFYTTPDPSTHSSGRY
                     EFSARGTGLGLAIAKSYVEAH"
BASE COUNT          616 a          652 c          776 g          590 t
ORIGIN      
        1 gggtttttcg attgagcagc gaactctttg ccagctataa caccacgggt ttgacccgtg
       61 gcatggtgac cggggtgggc gaagcttcag acggaacatt gtgggtggct tcgatgacca
      121 ccgggctttc gtcgctcagt ccatctgggg aaatgcgccg ctacaccagc aaagacggca
      181 ttctggaaga aaacaacctg tggtgcctcc tggttgatcg gcgagatcgc gtgtgggtcg
      241 gtggttacca gggtgtttcc tgttttgatg gtcagcggtt tgttcagttt ccgcgcgaaa
      301 agatgggggc tcaagaccgg attaccgcaa ttggcgaaga tcgccagggc catatctggt
      361 ttggatcggc caccagcacc agcaatggcg tagtgcggta tgacgggcaa accttccagc
      421 gatttactga ccaggatgga ctcagcagca atcaggtctc aggaattcgg ctcgatcaaa
      481 aaggagacat ctggttttgt accgaaaatg gattgacccg gtatgacgga cagcggtttc
      541 aggcatttac ccagaaagat ggacttcctt cgcgtcgtgt cagaacgctc taccaggacc
      601 atgacggcac ctattgggtt gggaccacgg atggtttgtg ccggtttgac gggacgcggg
      661 tgactcagat cttcaaggaa aaagaagggt taccggggag tctgattcga accatccagc
      721 gctttgacaa aactctgtgg attggcaccc agcgggggat tgccgaattt gacggacagc
      781 aggtggtccg ggtgtattcg gttcgggatg ggctggtcaa tgatgaaatt atcaataatg
      841 cctgtctcca gcgacgcgat ggcagtgtct ggtttggcac gaccgaaggc gtggttcggt
      901 atcgagtggc gcaggaaatt gcgcatccga tgcctcctaa agttcacctg accgggattc
      961 gtgtgaccgg tgcgggggcg ccgaatctgg tcgcaccagg gaacaaccag tcagcaccgc
     1021 tcccaaccct tgattatgac cagaatagcc tgacgtttga gtttctgggg ttgacctttc
     1081 ttgatgaaac agccgtgcgt tatacctacc ggcttgagcc ctttgatcgg gagtggtcag
     1141 cgccaagccc ggaacgtacg gctcgattta ccaatctgcc gcctggccgc tatcgctttg
     1201 cgattaaagc ctgttccgtg cgcgaaacct ggagcgagcc acagtatttc gaaatcacga
     1261 ttcgcccgcc cttctggcaa acctggtggt tccgcgccct gagtgttgca gcgctgatca
     1321 cacttatttc ggcactgtat acctggcgca ttcatgcctt aaatcagcgg caacagcaga
     1381 aacttgagca tttgcggcaa ttgcaggaac agcgaatgat ggtcatgcgg caattgctcg
     1441 aaagtatcca ggtgatcaat tcacaactgg aaaccaccgc tgtgctgcaa aacatcgccg
     1501 aggaaagcgc ccggctggtg gatggcaaac ctggcggcat tggactggtt caggatgacc
     1561 gggtggtgtt taaacggcag tgggtcaatg gaagctggca ggccggtgat gtttcgttcc
     1621 agtttgggga aggcacgccg ggaaaagtcg cgaggtcagc caaagcgatt attgtgaacg
     1681 acccggatga tgagcccgtc agttttgtgc cggaacaggc ccgcaaaaac acatctgatg
     1741 gatggatgga tgtgccgatc ctcacccgtg ctggcgaagt ggcagcggtg cttgatattc
     1801 gtcaccgacc agggcggcct ccgtttacca atgaagacat cagcctgatc gaatcgctgg
     1861 cgagtcaggc ggctgtggcg attgaaaacg ccagtttgta tggcgaactg gaagaaaaga
     1921 atttgatgat tgccgaatcg ttccgcgaac tggaacggct ctatcaacag gaacaggaag
     1981 tgtcgcgcac cctgcaggaa ctcaaccaga tgaagaccaa tttcattgtg gtcacctcgc
     2041 acgagatgcg aaccccgctg acagtcattc gcggctataa cgaagcctta ctgggtgaat
     2101 tttttggtga attaaacccg atgcagcaca aatcgctttc ggtcacccag cggatgattg
     2161 accgcatggt caatagtttc aacgacatcc tggagatgct caagatcaac gaaggccagt
     2221 ttacactcaa gcgcgagccc gccaatctca aggacctggt tcaggcggtt ttggaggaac
     2281 tccaaccttt tgttgaaaaa cgaaagcaaa ccctgacgat tgtcggaccg gatgtgatgg
     2341 ccacggttga tcgggagaag tttggtctgg tgttgctcaa tgtggtgcaa aacgccatta
     2401 aattcaccca ggacaccggc acgattcaaa tttcattggg gcgcgaaggc gaatcagctc
     2461 acattcgggt gattgattcc gggatcggaa ttgacgccag tgagttagaa cgcatcttcg
     2521 acaagtttta caccacgccg gatccttcga cgcatagttc cgggcggtat gagttttcag
     2581 cgcgcggcac agggctcggg ctggcaattg ccaaaagtta tgtcgaagcc cacg
//
LOCUS       DAOQDO010000735         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33488_length_2628_cov_3.010882, whole genome shotgun sequence.
ACCESSION   DAOQDO010000735 DAOQDO010000000
VERSION     DAOQDO010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33488_length_2628_cov_3.010882"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PKZ53_12150"
     CDS             complement(<1..1271)
                     /locus_tag="PKZ53_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41236.1"
                     /translation="MGRRILVADDSITVRKSLQAFLIEQGHEVVAVGNGDLATRRAGE
                     MRPELALIDILMPGKNGYEVCRHIKQTLGLPIPVMLLRSDFEPFDTRESVRVGADQVL
                     LKPIDQDQLVAAIRDAWIAYGFTDDEEPPPPEASWSDVAEEVESIPEAEDGFSPEVFA
                     SHQDLDGFLSPDVVAAGLNLAPVPTSDFSETADSDNPSLLAPPPLESSWETTPATTAS
                     EYVSSSWMSPIPESPWSEPGTAAASSYAVASETPASASSVLTLTSTAESVSPAYLGPP
                     TAELEDPFTVVSRGEYKSFFKDLLRTTGAHDAVSLPTDPAFIEQALSELSSEDAALFA
                     AMMGTSVEGLRQRAAGVSDPHPTPNPFAPLPPVSIPTQDFVVPLNQTTEIVVDPTGGA
                     TISFDQTQGTVQIHGLVQEDGSSLPVSEVEIP"
     gene            complement(1573..>2628)
                     /locus_tag="PKZ53_12155"
     CDS             complement(1573..>2628)
                     /locus_tag="PKZ53_12155"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HNJ41237.1"
                     /translation="ISPDLVRKVLSLIDGTRTIGDCVARCDGDLDQLRILAALWSPEI
                     RKTLLPPEPQAPPPPPPPLVAQPLQPNPSGGYPMPGPEPLTPPAPPMNPGYGGGMPPM
                     AVPPMSPQPGPPQPGGWAQSGMFPSADLFPQAPPQQPGNWGQSGTYPTAPDQQPYPPQ
                     GYPGYPQGYPPSPYPGYSGGYPSPYGSPYGAPPPYPPSQMPQYPAYPPGTGYPGVPQQ
                     PGYPGGYPGGTVPQPPGYPGQPPQMPSVTPSSPPQSPMNTPSKPAGGQSSGPVDVSIV
                     FQDPQSEVQLAQKLYEAVVLAKRQCGEAVDEFTFQRFHRILVEQSSKIKGQLNCPRVV
                     FSVQVEGGRVKFIGKGA"
BASE COUNT          603 a          710 c          744 g          571 t
ORIGIN      
        1 gggatttcaa cctcgctcac gggcagtgaa cttccatctt cctgcaccag gccgtggatt
       61 tgaactgtcc cctgggtttg atcaaaagaa atggtggcgc cacccgtcgg atcaacgaca
      121 atttcggtcg tttgattgag cggaacaaca aaatcctggg ttggaatcga aaccggtggc
      181 aacggagcaa atggatttgg cgtaggatgc ggatcactga caccagcggc ccgctggcgc
      241 agcccttcaa ccgacgtgcc catcatggcc gcaaacagtg ccgcatcttc cgaagataac
      301 tcacttaatg cctgctcaat aaaggctgga tccgtgggca atgacaccgc atcgtgggca
      361 ccagttgtgc gcagcaaatc cttaaaaaat gacttgtact ctccgcgcga aaccaccgta
      421 aaggggtctt ccaattcagc cgttggcggc ccgagatagg ctggggagac tgattcggca
      481 gtcgaggtga gggttaaaac cgagcttgcc gaagccgggg tttcgctggc aacggcatac
      541 gaggaagcag cggcagtgcc gggctcactc cagggagatt caggaatcgg cgacatccag
      601 gaactggaaa catactcact ggcggttgtg gcaggtgtcg tttcccagga tgattcaagc
      661 ggtggcggag caagcaagga cggattgtca gaatcagccg tttccgaaaa atcactggtc
      721 ggcacgggtg ccagattgag accagccgcg accacatctg gcgataaaaa cccatccagg
      781 tcctggtggc tggcaaaaac ttctggagaa aacccatctt cagcttccgg aattgattca
      841 acctcttcgg caacatctga ccaggaagct tccgggggtg gtggctcttc atcatcagta
      901 aacccatagg caatccaggc atcccgaatg gccgccacca gttggtcctg gtcaatcggt
      961 ttcaataaca cctgatcagc accgacacgg acagattccc gagtatcaaa cggctcaaag
     1021 tcgctccgta ataacatcac cggaatcggg aggcccaggg tttgtttaat gtgacgacag
     1081 acctcatagc cgtttttgcc gggcatcaga atatcaatca atgccagttc cggcctcatt
     1141 tcacctgccc gccgggtcgc gagatcgcca ttgccaaccg ccacaacttc gtgaccctgt
     1201 tcgattaaaa atgcttgcag acttttgcga actgtaatgc tgtcgtctgc gaccaaaatt
     1261 cgtcttccca tacccacctg ttcggacttc ccttgaaaat atcccttcgt tcaacacttc
     1321 caggcacagc catccccaaa gacaatctgg gttccgctca agcctacgta gttcacctgt
     1381 tgggaatctt gaaaaagaga tgcagacttg tttgtatcaa aaaaatgagg ggagattcaa
     1441 tcttatacca gttcggagtc agtagtcagt agtcagtagt tcattaagtc tatttggttg
     1501 aatcacttga ctattttcta gcggcaactg gtgaaagcgg tgtcaggcgg ccacgctcca
     1561 cacctgctgg catcaggcac ctttcccaat aaacttgacc cgacctcctt caacctgaac
     1621 cgaaaacaca acccgagggc aatttaactg gcctttgatt ttggaggatt gctcgaccag
     1681 gatgcgatga aatcgctgga aggtaaactc gtccaccgct tctccgcact gccgtttggc
     1741 cagaaccact gcttcataga gtttttgagc cagttgtact tcgctttgtg ggtcctgaaa
     1801 tacaatactg acatcaaccg ggccggatga ttgcccgcct gccggtttcg atggggtgtt
     1861 catcggggat tgaggcggcg atgacggtgt cacggatggc atttgggggg gctgcccagg
     1921 ataccccggc ggttgaggaa cggttccacc cggatagccg cccggatagc ctggttgctg
     1981 gggaacacct gggtatccag tacctggtgg ataggctgga tactgcggca tctgacttgg
     2041 cggatatggc ggaggtgcac catacggtga accataaggc gaaggatagc cgcccgaata
     2101 gcccggatat ggcgacggcg gatacccctg cggatacccc ggataccctt gcggaggata
     2161 tggttgctgg tctggcgccg tcggataggt gccagattgc ccccagtttc ctggctgctg
     2221 gggtggtgcc tggggaaaca aatcagccga cgggaacatc ccggattggg cccatccacc
     2281 tggctgcggt ggtcctggct ggggtgacat gggcggcacc gccatcgggg gcattccccc
     2341 gccataccca gggttcatcg gaggcgcagg tggggtcagc ggttcgggtc ccggcatcgg
     2401 atatccaccc gatggatttg gctgcagtgg ttgggcgaca agcggtggcg gtggcggtgg
     2461 cggcgcctga ggttcaggtg ggagcaatgt ttttcgaatt tcaggcgacc agagcgcggc
     2521 caaaattcgc aattgatcca aatcaccatc gcagcgcgcc acacaatcac cgatggttcg
     2581 ggttccatca atcaggctga gaacttttcg gaccagatct gggctgat
//
LOCUS       DAOQDO010000736         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33507_length_2627_cov_3.917963, whole genome shotgun sequence.
ACCESSION   DAOQDO010000736 DAOQDO010000000
VERSION     DAOQDO010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33507_length_2627_cov_3.917963"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2250)
                     /locus_tag="PKZ53_12160"
     CDS             complement(88..2250)
                     /locus_tag="PKZ53_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41238.1"
                     /translation="MSEHLNFTHLLRQRLFLALTVLCTSIFFTPSLFAQNPTTIDLGV
                     KIPELGIYGASANDATGNSVAVGDLNGDGLNDVVVGSPRERGPANDPTGRLGSGVVRV
                     FLAPFAANDQVDLNQRQAPVTIYGQQTGDGLGSNLLIADINADNRPDLIMQAANADFG
                     GTRSDAGKVLVLFGPITPQTRDLANPQTPPNVQFIGMSAGDRVGNSLAAGDFDSDGRT
                     DLIIGSPFSTVNGAPQGGAIFIIYGRPSASFPASYDLTQFAQNQDDAFGFQTADTQFG
                     FSVGFGDVNGDRRGDIIAGAPRFDGANGARLNSGAVVVLFNSELQNPFIGFGIDTNDL
                     MGSSISVGDVTGDSLADVIASAPGAFSLSNQRSGAGEVYMLLGGRFAPGQIRDLLIQG
                     QGPEATFFGAAIGDRLGFPFGTAVGDINGDTIGDILMVANGGDGPNNSRIDAGNVYVV
                     LGNAGLAGPRDLATPSTSNVIVYGARSQDQIGSPIETAPSNRTGTPVVIGDISGDRKG
                     DLVLGCPFSDGQIAAPKTDAGVVYALTQLSGPPPVNQPPVITPVANQSVVEGRSLSVA
                     ISATDPNGDTIRFSLTGPAFVQVFDNFNGTGTIQIMPPINTPKQTYSIVLTATDAGTP
                     ALNSAVTFTVTVTSAGPVINSVMYDGKFMTISGVGLTPSPTVRVNGNVLATKFLKTIT
                     DQQLKVKGKPAKLKLQSGDNQIIVVVNGLESNVAILRL"
BASE COUNT          636 a          691 c          675 g          625 t
ORIGIN      
        1 gataattttc aagtaccttt ttatttatga tactttcata attttcggaa ttttcggagt
       61 gcggcggctt gacgccgctt ttgttttcta aagtctcaaa atagcaacat ttgactccag
      121 cccgttgacc accacgatga tctggttatc acctgattga agcttcagtt tggctggttt
      181 gcctttgacc ttgagttgct ggtcggtgat ggttttgagg aatttggtcg ccagtacatt
      241 gccattaacc cgcacggttg ggcttggcgt caacccgacg ccgctgatgg tcataaactt
      301 gccgtcatac atcaccgaat tgatcaccgg tccggctgag gtgacggtga cggtgaaggt
      361 cacggcgcta ttgagggccg gagttcccgc atcagtggcc gtcaaaacga tgctgtaggt
      421 ttgtttcggt gtgttgattg gcggcatgat ctggatcgtc cctgtgccgt taaagttgtc
      481 aaacacctgt acaaacgccg ggccagtcag gctaaaccga atggtatcac catttgggtc
      541 agtcgctgaa atcgcgacac tcagcgatct tccttcaacc accgattggt tggctaccgg
      601 agtgatgacc ggtggctgat tgactggcgg tggcccgctc aattgggtca gtgcatacac
      661 cacgccggca tcagtttttg gcgccgcgat ttgtccatcc gaaaacggac atcccagcac
      721 caggtcacct ttgcggtcac ctgaaatgtc cccgatgaca accggggtac cggttcgatt
      781 gctcggtgcg gtttcaatcg gagacccaat ctgatcctgt gagcgggcgc catacacgat
      841 cacatttgaa gttgacggtg tcgccagatc acgtggccca gccagtccgg cgttcccaag
      901 caccacatac acgtttccag catcaatccg gctgttgttt ggcccgtctc cgccattggc
      961 aaccatcaaa atgtcgccaa tggtatcgcc gttgatatcg ccaacggcgg ttccaaatgg
     1021 aaacccaagg cggtcaccaa tcgccgcgcc aaaaaatgtc gcttctggtc cctggccctg
     1081 gattaacaga tcccgaatct gacccggagc aaagcgtcca ccgagtaaca tatagacttc
     1141 gccagcccca cttcgctgat tactgaggct aaaggcaccg ggggcgctgg cgatgacatc
     1201 ggccagactg tcgccggtca catcgccgac cgaaatgcta ctgcccatca ggtcgttggt
     1261 atcaattcca aagccgataa acgggttttg tagttcacta ttaaagagca cgacgaccgc
     1321 gccgctgttg agtcgggcac cattcgcccc gtcaaaacgc ggtgctccgg caatgatatc
     1381 cccacgccgg tcaccgttga catcaccaaa cccgactgaa aagccaaact gggtatcagc
     1441 ggtttggaat ccaaaagcgt cgtcctgatt ctgggcaaac tgggttaagt catagctggc
     1501 ggggaagctg gcacttggac gaccatagat gatgaaaatc gcgccaccct ggggggcgcc
     1561 attcacggtt gaaaagggtg atccaatgat caggtcggta cgtccgtcgc tgtcaaaatc
     1621 acctgctgcc agtgagtttc caacccggtc accggctgac atcccaataa actgaacatt
     1681 cggaggggtt tggggatttg ccagatctcg cgtttgtggt gtgatcgggc caaacagaac
     1741 cagcactttg ccggcgtcgg atcgagtgcc accaaaatcg gcattcgcgg cctgcataat
     1801 caaatccggg cgattatcag cattaatatc cgcaatcaac agattgcttc caagaccatc
     1861 gccggtttgc tggccataaa ttgtgaccgg cgcctggcgc tgatttaaat caacctggtc
     1921 gttggcggca aatggtgcca aaaagacccg aacgacacca gagcccagcc gtccggttgg
     1981 gtcgttggct gggccgcgtt cacgtggact cccaaccacc acatcgttca agccatcgcc
     2041 attgaggtct ccgaccgcaa cactattgcc cgtcgcatca ttagccgaag cgccatagat
     2101 accgagttcg gggattttta cccccagatc aattgtggtt ggattttgag caaaaagtga
     2161 tggtgtgaaa aaaatactgg tgcaaagcac ggtcagagcc aggaacagcc gctgacgcaa
     2221 taggtgtgtg aaattcagat gttcagacat agggtgggat cctcctcgat tggttcaatt
     2281 gacccaagac gcaaggggcg aaatcaaatc agttgagttg catcagtgcg gagcgcacgt
     2341 gagaaaagtg tgagaaaaac tgaggtgtga gtgattttct gctttttatg cgtgtagaag
     2401 caatcaaaaa ttaactgaac caggaatttt aagggcaaag ttcacgaaaa cacgattctt
     2461 ctctcttgta tgcgccaaac ccgtgtttct cagctcatcg ggtgcctcct gctataaaga
     2521 tttgtgcaaa aaaataacct tccgccactt cacccagttg taaaaagtaa aatcggattt
     2581 ataataattt tctgcagtat actgatagct attacaccga cattgac
//
LOCUS       DAOQDO010000737         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33529_length_2626_cov_3.605601, whole genome shotgun sequence.
ACCESSION   DAOQDO010000737 DAOQDO010000000
VERSION     DAOQDO010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33529_length_2626_cov_3.605601"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKZ53_12165"
     CDS             <1..846
                     /locus_tag="PKZ53_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ41239.1"
                     /translation="KPFTATELFNALQQAIECETGFRGSVHGLSLTDVLQMFHFGRRS
                     VVIQVGGRVPGRLFFQDGELIHAQIADVVGEAAFKMILQLPSGSIQTSVFTEAPTTIR
                     KPFDNLLLDSLRTIDEEAVGSESEDQVDDELDRSFAPSSVPPAQNGQMTSQTQSSHQQ
                     SPDEIVEKWNQACTTLGFEFERMQAIAFTTGNEHPIVLQGPADMVSDREAVVGLVASV
                     AQLCPDSTTGIFEYVTHGVGCALIWNVEDQFIVMVSNTVQEPSEVAFFRRNITILGRA
                     LTQEN"
     gene            855..1922
                     /locus_tag="PKZ53_12170"
     CDS             855..1922
                     /locus_tag="PKZ53_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ41240.1"
                     /translation="MNHQNCDNQPGIVLTKEAVCAFELIPFPVMVVSLDSVIAYTNPA
                     LQALVEQIYPDGTPQRLDDFGFSELTEALHQFAASGDQSAKIALSAWGHHPAQSFWLL
                     HLSRQECTFLSGPGVCVIFECAPGPESGSNLPTDFLGGLIHQIRNPIFAISSTLDAFE
                     ARFGVSPDQEPFVAVLRAEQRRLMTILHEVLEFDSPLPVEFSEAPFLSTLQCAIDHCV
                     LLASDRNVTLELVVSGDLPSFHMDSQALQQAWILLIEGAIHASTPNQQVTIRTQPEIY
                     NSQPGVAVEVFDQGPFLKECDLDRFCEPFVSKRCGESGLGFSIVKRVIESHCGKLTAA
                     NLPNGLVQIKTWLPVRYGKAQ"
     gene            2197..>2626
                     /locus_tag="PKZ53_12175"
     CDS             2197..>2626
                     /locus_tag="PKZ53_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41241.1"
                     /translation="MEFINFQWARHKACQGWLSLICLGVCVPGWIYPVLADQDPVLAT
                     RKRIIGDAPQSAPTQNQDPQGIEATAGGIITVEAESRQVTFSVLDRNHRLVKDISASD
                     FELYDGGERQEVDVFTTGKGLPVMLAVVVDMSASQESLLPV"
BASE COUNT          599 a          620 c          694 g          713 t
ORIGIN      
        1 aagcctttta ccgcgacgga actgtttaat gcactccaac aggcgattga atgtgaaacg
       61 ggatttcggg gaagtgtgca tggcttgtca ctgaccgatg tcttgcagat gtttcatttt
      121 ggtcggcggt cagtggtgat tcaggttggc gggagagtgc ctgggagatt gttttttcag
      181 gatggagagt taatccacgc ccaaattgcg gatgtggttg gtgaagcagc ctttaaaatg
      241 attttgcagc ttccatcggg ttcgattcaa acctcagttt ttaccgaagc gccaacaacg
      301 atccgcaaac cctttgataa tttattactt gactctcttc ggaccattga tgaagaagcc
      361 gttggaagtg agtctgaaga tcaggttgat gatgaattgg atcggagttt tgcaccctcg
      421 tcagtcccac cagcccagaa cgggcagatg acttctcaga ctcaatcctc acatcagcaa
      481 agccctgatg aaatcgtgga gaaatggaac caggcctgca ccacgctcgg ttttgagttt
      541 gagcggatgc aggcgattgc atttaccacc ggaaacgagc atccaattgt tttgcaggga
      601 ccagccgata tggtttcaga tcgtgaggca gtggttgggc tggtcgcttc agttgcccag
      661 ttatgcccgg attccacaac cgggatcttt gaatatgtga ctcacggtgt ggggtgtgcc
      721 ttaatttgga atgtggagga ccagtttatt gtcatggtga gcaatactgt ccaggagccg
      781 agtgaagtgg cgttctttcg gagaaatatc accattttgg gtcgggctct gactcaggag
      841 aattagctgg ctatatgaac caccagaatt gcgataacca gcccggtatt gtgttgacga
      901 aggaggcagt ttgcgccttt gagcttattc cgttccctgt gatggtggtg agtttagatt
      961 ccgttattgc ctacaccaat ccagcgcttc aagccctggt tgagcagatt tacccggacg
     1021 gcactccgca gagattggat gatttcggtt tttcagaatt gactgaggct ctgcaccaat
     1081 ttgctgcctc tggagatcaa tccgcgaaaa tcgccctttc tgcctgggga caccatccgg
     1141 ctcaatcttt ttggttgctt cacctctcgc ggcaggaatg caccttttta tctggcccag
     1201 gggtgtgtgt gatctttgaa tgtgcgcctg gcccagaatc tggctcaaat cttccaaccg
     1261 acttcctggg tggtctgatt caccagattc gaaatcccat ttttgccatc agctcaaccc
     1321 tcgatgcttt tgaggcacga tttggcgttt ccccagatca agaaccgttt gtggctgttc
     1381 tgcgagctga acagcggcga ttgatgacga tattgcacga ggttttggag tttgactcac
     1441 ccttacccgt tgaattttcc gaagcaccat ttctatcaac cctccaatgt gccatagacc
     1501 attgtgtact gctggcgagt gaccgcaatg ttaccctcga acttgttgtc tcaggtgatc
     1561 ttccatcgtt tcacatggat tcgcaggcac tccagcaagc ctggattttg ctgatcgagg
     1621 gcgccatcca tgcctcaact cccaatcagc aagtgacgat tcggacgcaa ccggaaatat
     1681 acaactcgca accgggtgtg gcggttgaag tgttcgacca gggtccattt ttgaaggaat
     1741 gcgacctgga tcgtttttgt gaaccctttg tctcaaaacg atgtggtgag tccgggcttg
     1801 ggttttcgat tgtgaagcgg gtgattgaga gtcattgcgg aaagctgacg gcggccaatc
     1861 tccccaatgg tctggttcaa atcaaaacct ggcttccggt gaggtatggc aaagcgcaat
     1921 aacaggttgg aaaacacagt ctcagcctgc cttgcttttc accaccagca tacctgcccg
     1981 tctcgaatct gaatttcttc ctcgcggctc aatgtaatca atccccacct cagcgcatgt
     2041 tcatctgttc aagggaaaca accgatttgc gatatctgtg aatcagagtg aacgcatttc
     2101 tcaaatcggt tgacgtttag cctgtcatac caatccaaat cagaacgtgt tgtttcctga
     2161 aaccagattt cgggcctggg tgaggtgtcg atcaatatgg aatttataaa ctttcaatgg
     2221 gccagacaca aagcctgcca ggggtggttg agtctaatat gtttgggggt atgtgttcca
     2281 ggctggatct atccggtact ggctgaccag gatccagtac tggctacccg caaacgaatc
     2341 attggtgatg cgccacaatc tgcacccact caaaatcagg atccgcaggg gattgaagcc
     2401 acagccgggg gaatcattac ggttgaagct gagtcccgtc aggtcacttt ttcggtattg
     2461 gaccgcaatc atcggctggt gaaagacatt tcagcctcgg attttgaact ctatgatggt
     2521 ggcgagcgcc aggaagtaga tgtgtttacc actggcaaag gactgccggt gatgctggca
     2581 gtggtggtgg atatgagtgc cagtcaggag tctttattgc cagttg
//
LOCUS       DAOQDO010000738         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33536_length_2626_cov_2.592377, whole genome shotgun sequence.
ACCESSION   DAOQDO010000738 DAOQDO010000000
VERSION     DAOQDO010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33536_length_2626_cov_2.592377"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..>2626)
                     /locus_tag="PKZ53_12180"
     CDS             complement(43..>2626)
                     /locus_tag="PKZ53_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ41242.1"
                     /translation="DGISNNDNYSGTVAQGQEGVNVKSGGFLPVDAVQEFTLTYAPSA
                     QYGSKAGGLVNVAIKSGTNEFHGSLYEFFRNDKLDARAFFNATRDSNTGAPLAKNPLR
                     MNQFGFTLGGPIVKDKLFFFGNYEGQRVRQAESLTVFSPGAASIARATPNGGINPLSA
                     RLLSLFPLGDANDSAVVTFPSFSDIDNYIVKLDYNINANNTINGKYLLGNTSQSEVDN
                     FYLRPEDIASNDQRSQSLGVSYTSVIKPEIVNEVRFGYTRLFQEIAPLNKDFNPADIG
                     FVTGITDPTRGGMPRIRISGFDTFGGRATNLISDPSETYSILDNITWTRGRHSFKFGG
                     DFRYDRAVNTRDLAARGEFRFRSLGDFLAGRVDRASVLTGSTLREVSQKSWSLFAQDD
                     FKIRPNLTLNFGVRYEFFGVLKEANNLLANFIPGVGLRQVGAGLDKPYENDLNNFAPR
                     LGIAWDVKGTGRTVVRANWGMYYDPPALSAFIGQTGTVNAISPTLGLNFNPVGSINSY
                     IVRPRGSSLNWRLGQQIFPSLEIPDSFLDVLGIDQNIRTPFTHSYSLNIQQKLWDNGS
                     LEVAYVGNRGTRLYSLSDINQIFDVENELRPFDNQLLNADGDPQFQYVNFLTSTGNSN
                     YNALQATFTQRNWRGVTALIGYTYAKSMDTASSNRPVNPQNSNNIRAERARSDFDVKH
                     RFTGSVSYEIPTMTFLPTVLGKGWAVNTLMTFQTGKPVDVFYGDDFSGFFEFNDRPNI
                     IGDLSLIKFQPGQRLDRNILNQVFAAPDFGTFGNAGRNILTAPGFTIVDFSVTKNTQL
                     TERLKMQFRAEFFNIANNPEFAQPRGDLLNSQFGRLGDTADRGNPLSSGGPRRIQFAL
                     KLTF"
BASE COUNT          629 a          691 c          697 g          609 t
ORIGIN      
        1 ttttttctca aaccctgaac cctgaaccct gaacccttga ttttagaaag ttaacttcaa
       61 ggcaaactga atccgacgcg gaccacctga ggacagtggg ttgccgcgat cagccgtgtc
      121 gcctaaccga ccgaactgcg agttcagtaa gtcgccacgt ggttgggcaa attccggatt
      181 gttggcaatg ttgaagaatt ctgcccggaa ctgcatcttt aaccgctcag tgagttgggt
      241 gtttttggtc accgagaaat ccacaatcgt gaagcctggt gcggtcagaa tgttgcgacc
      301 agcattacca aaggttccaa aatcgggggc ggcaaacacc tggttcaaaa tattgcgatc
      361 cagccgttga ccgggttgga atttgatcag gctcaaatcg ccgatgatgt ttggacggtc
      421 gttgaattca aagaatcctg agaaatcatc accataaaac acatccactg gcttcccggt
      481 ctggaacgtc atcaacgtgt tgacggccca gcctttgccc aaaacggtcg gcagaaacgt
      541 catggtcgga atttcatatg acacgctgcc ggtaaagcga tgtttgacgt caaaatccga
      601 gcgagcgcgt tcagcgcgga tgttgtttga attctgtggg ttcactggac ggtttgacga
      661 tgccgtgtcc attgatttgg cataggtata cccaatcagg gccgtcaccc cacgccagtt
      721 acgctgggta aaggtggctt gcaacgcgtt gtaatttgaa ttgccagtcg aggtcaggaa
      781 gttgacatac tggaattgcg gatcgccatc ggcgttgagc aactgattgt caaacggacg
      841 gagttcgttt tcgacatcga aaatctggtt gatatctgac agcgaataga gccgcgtccc
      901 acgattgcca acataggcca cttcgagcga gccattgtcc cacaattttt gctggatgtt
      961 gaggctgtag ctgtgggtaa acggcgtgcg gatgttctga tcaatcccga gcacatccag
     1021 gaagctgtct ggaatttcca gcgatgggaa gatttgctgg cccagtctcc agttcaggct
     1081 cgaaccacgt ggacgcacaa tgtagctatt gatcgaaccc accgggttga agttcagacc
     1141 cagggttggt gaaatcgcat tgaccgtgcc agtttgtccg atgaaggccg agagtgccgg
     1201 cggatcataa tacatccccc agttggcacg aactacggtc cggccagtcc ccttcacatc
     1261 ccaggcaatc ccaaggcgtg gcgcaaagtt gttcaaatca ttttcatacg gcttgtcgag
     1321 accagcccca acctgtcgca aaccgacccc tggaatgaaa ttcgccagca ggttgttcgc
     1381 ttccttgagc acaccgaaaa attcatagcg caccccaaag ttcagggtca ggtttggacg
     1441 gattttgaaa tcatcctggg caaacagcga ccagctcttc tggctcactt cgcggagcgt
     1501 gctcccggtc aaaactgacg cccggtctac ccgaccggcc agaaaatcac ccagcgaacg
     1561 gaagcgaaat tcaccacgcg ccgccaggtc acgggtattg accgcccggt catagcggaa
     1621 atcaccaccg aacttgaacg agtgacgacc acgcgtccag gtaatgttgt ccagaattga
     1681 ataggtttcg cttggatccg aaatcagatt ggtggcacgt ccaccaaagg tatcaaaccc
     1741 gctgattcga atccgaggca tcccaccccg ggttggatcg gtaatcccgg tcacaaatcc
     1801 gatgtctgct gggttgaagt ctttattgag cggagcaatt tcctggaaca gccgggtgta
     1861 gccaaaccga acttcgttga cgatttctgg tttgatgacc gacgtgtagc tgacgcccag
     1921 actctgtgac cgctgatcat tggacgcaat gtcttccggg cgcagataaa aattatccac
     1981 ttcggactga ctggtgttgc ccaacaggta tttgccgttg atcgtgttgt tggcgttgat
     2041 gttgtagtca agcttgacaa tgtagttgtc aatatcgcta aacgatggaa atgtcaccac
     2101 ggcgctgtcg ttggcatcac ccagcggaaa cagtgacagc aaccgggcgc ttaaggggtt
     2161 gattccacca tttggggttg cccgggcaat tgaagccgca ccgggtgaaa acaccgtcaa
     2221 actttcggcc tgacgcacgc gctggccttc atagttgcca aaaaagaaca gcttgtcttt
     2281 gacaatcgga ccacccaggg taaagccaaa ctggttcatc cgcaggggat tcttggccag
     2341 tggcgccccg gtgtttgaat cgcgggtggc attaaaaaac gcccgggcat cgagtttgtc
     2401 gttgcggaag aattcataga gcgaaccgtg gaactcgttg gttcccgact tgatcgccac
     2461 gttgaccaat ccaccggctt tcgacccata ttgggctgat ggagcatagg tcagggtgaa
     2521 ttcctgaacc gcatccaccg gcaaaaaccc gcccgacttg acgttgacgc cttcctgccc
     2581 ctgggcgacg gttccagaat agttatcgtt gttgctgatc ccatcc
//
LOCUS       DAOQDO010000739         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33552_length_2625_cov_3.715953, whole genome shotgun sequence.
ACCESSION   DAOQDO010000739 DAOQDO010000000
VERSION     DAOQDO010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33552_length_2625_cov_3.715953"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKZ53_12185"
     CDS             <1..439
                     /locus_tag="PKZ53_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ41243.1"
                     /translation="GWSASDTVRHLEKSTKPVVTALHLPGLALRATFGMATNPSISYN
                     ELVARYQEALKNGGTAGRFSPSEQELPENLQTWQSDVITNCRKALADLSSVLEGWTEK
                     KLDQYQLPHPLIGKLTVREMMLFTIYHYRHHQGIIARKVQSKG"
     gene            648..1199
                     /locus_tag="PKZ53_12190"
     CDS             648..1199
                     /locus_tag="PKZ53_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNJ41244.1"
                     /translation="MELDHAQPYIIKIRNLLSSDECQQLINRFEADTPSLATVNTVYG
                     AQVRLGVRNNERVMFTDFELATMLFQRAEPHVPDEIQGLKLFGVNELFRCYRYKPGMR
                     FAPHPDGAFRRNECEQSCFTFMVYLNDDFEGGHTTFLVTPEVSVKPLTGMALFLQHPL
                     IHEGSLVTKGVKYVLRTDVMYHI"
     gene            1232..1804
                     /locus_tag="PKZ53_12195"
     CDS             1232..1804
                     /locus_tag="PKZ53_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41245.1"
                     /translation="MTSQDVPTKSTPASQIWYSISGIWILLVFVIANWLSLTIGFELS
                     EALIPVQLWETTPWGVIRQDYIQGLCHAVALLFCLSLGLAMLERYRAIASITIWMSFM
                     TFSPNVWKMMVIRSRCQYVMSTGNATSSWLSFDSYFQDPLIQRGPLVAIFIVFVLAMI
                     LIFLEYRHNIKNIAKPVEKLTPSEPIPPQL"
     gene            1832..2383
                     /locus_tag="PKZ53_12200"
     CDS             1832..2383
                     /locus_tag="PKZ53_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41246.1"
                     /translation="MNLDNIYPYLMPGLIGPEFETVSIPLGHDVYVVIFEDHEADAGI
                     VHSIVTPYKLQVSGLDIAHLHQRAMQNLEQFAERDDIGIKMLGQPGEEVNFLLFHGHA
                     RASACLLLSNLYGLAQNWLESDELCACVPQRETLVVFPKRDRAYREFLIGKLKEIEGD
                     ARRPITFELFELTPDGVVPFVEP"
     gene            2399..>2625
                     /locus_tag="PKZ53_12205"
     CDS             2399..>2625
                     /locus_tag="PKZ53_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41247.1"
                     /translation="MKTLDELLWPDDSNATLHDAELLNLTIDYVQGMAEFTLSICVGD
                     PESEDALIYKARQTGILTVSGLEMLSIEPPT"
BASE COUNT          681 a          640 c          619 g          685 t
ORIGIN      
        1 cggatggtcg gcatccgaca cggttcgtca ccttgaaaaa tccaccaaac cagtggtgac
       61 agcgctgcat ctgcctgggc ttgccttgcg ggctacgttt ggaatggcga ccaatccatc
      121 catttcatat aacgaacttg tcgcccgata tcaggaagcg ttgaaaaatg gcggcaccgc
      181 cggacggttc tcgccgtctg aacaagaact ccccgaaaat cttcagacct ggcaatctga
      241 cgtcattacc aattgtcgca aagccttggc tgatttgtct tcagtactcg aaggctggac
      301 agaaaagaag cttgaccagt atcagttgcc acacccactc atcggaaaac tcacggttcg
      361 ggaaatgatg ctgtttacca tttaccatta ccgtcaccat cagggaatca ttgcgcgcaa
      421 agtgcaaagc aagggctgaa aaaaccgggg ctgagaaaac cgggactgaa gacttcgggc
      481 tgaagaagtc gggctgaaga agtcgggctg aaaaacatcg ggctgaagaa gtcgggctga
      541 agaagtcggg ctgaagaagt cgggctgaag aactggtttt ctttcatccc tcatcccttc
      601 gattgccctg agcttgcaaa tcttcaaccc tcacaaagag gtttatcgtg gaactcgatc
      661 acgcacagcc ttatatcatc aagatcagaa acctcctgtc gtcagacgag tgtcaacagt
      721 tgatcaatcg ctttgaagcg gacactccgt ccctggccac ggtcaacacc gtctacggcg
      781 cacaggttcg gctgggagtt cgcaacaatg agcgggtgat gtttactgat ttcgagctgg
      841 caacgatgct ttttcaacgg gctgagccgc atgttccaga tgaaatccag ggcctgaagc
      901 tgttcggggt gaatgaatta tttcgctgtt atcgctacaa acccggaatg cgctttgccc
      961 cacatcctga tggggcgttt cgccgcaatg aatgtgaaca aagctgtttc acttttatgg
     1021 tttatctgaa tgatgatttt gaaggcggtc acacgacatt tctggttacg ccagaagtct
     1081 cagtcaagcc actgaccgga atggcactgt tcttgcaaca tccgttaatt cacgaaggca
     1141 gcctggtgac caaaggtgtc aaatatgtcc ttcgaactga tgtgatgtat cacatttaac
     1201 ttgatgaatt ttaacgtgaa aggagccaac gatgaccagt caagatgttc caactaaatc
     1261 aactccggca tctcaaatct ggtactcgat ttccggtatt tggattttac tggtattcgt
     1321 gatagcaaat tggctgagtt taaccattgg gttcgaactt tcagaggcat tgattccagt
     1381 tcagctttgg gaaaccaccc cctggggtgt tatccgccag gattacattc aggggctatg
     1441 tcatgctgta gccttattat tttgtcttag cctgggactg gcaatgcttg aacgataccg
     1501 agcgatagct tctataacga tctggatgag cttcatgacg tttagtccaa acgtatggaa
     1561 aatgatggtc atccggagtc ggtgtcaata tgttatgagt actggtaatg ccaccagtag
     1621 ctggctctct tttgacagtt attttcagga tccgcttatt cagcggggtc cccttgttgc
     1681 cattttcata gtctttgttc tggcaatgat tttgatattt cttgagtacc gacacaacat
     1741 caaaaacata gccaagccgg ttgaaaaatt gacaccttct gaacctattc ccccacaact
     1801 ctgaaatcct gctttgatga aagttggttt tatgaatctt gacaacatct acccatattt
     1861 aatgcctgga ttgatcggtc cagagtttga aaccgtcagt atcccgcttg gacacgacgt
     1921 gtatgtagtg atttttgaag accacgaagc tgacgccggg attgtgcatt ccatcgttac
     1981 cccatacaaa cttcaggtta gcggccttga tatcgcgcac ctccaccagc gagcaatgca
     2041 aaatctggag caatttgccg aacgcgatga cattggcatc aaaatgcttg gtcagccagg
     2101 agaagaagtc aattttcttc tgtttcatgg ccatgcgcga gcctcagcct gtttgctgct
     2161 gtccaatttg tatggccttg cccagaactg gcttgaatca gacgaattgt gtgcctgtgt
     2221 cccgcaacgt gaaacactgg tggttttccc caaacgcgac cgagcctatc gtgaatttct
     2281 gattggcaaa ctgaaagaaa tcgaaggcga tgcccgacga ccaatcacct ttgaactctt
     2341 tgaactgaca ccagacggcg tagtgccatt tgtcgaaccg taaaaggctc atcacataat
     2401 gaaaacactt gatgaattgc tctggccaga tgattcaaac gccacgctcc acgatgccga
     2461 gctcctgaac cttacgattg actatgtgca gggaatggct gagtttaccc tttcaatctg
     2521 tgtcggagat cctgaatcag aggatgcact tatttacaaa gcccgccaaa caggaattct
     2581 taccgtcagt ggcctcgaaa tgctatctat cgaaccacca accag
//
LOCUS       DAOQDO010000740         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33610_length_2623_cov_2.712617, whole genome shotgun sequence.
ACCESSION   DAOQDO010000740 DAOQDO010000000
VERSION     DAOQDO010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33610_length_2623_cov_2.712617"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PKZ53_12210"
     CDS             <1..1449
                     /locus_tag="PKZ53_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41248.1"
                     /translation="PAATVNAGPDQTICAGSTVSLAGSLGGSATTATWSGGSGTYSPG
                     FGALNATYTPSAAEVAAGSVTLTLTTNDPAGPCGTGSDTVTPTINPAAGVNAGPDQAV
                     CASNASVILAGILSGSATSGTWTGGSGTFVPNNTTLNATYTPTAAEIQAGTVTLILTT
                     NDPAGVCGAKTDSMTISISPSCSELAVLYVADTTNNRIQKFDGQAWSLIGGGAGSAAV
                     GQFKRPEAVVANVAGTRIYVADTGNNRIQWSTDGGATWNLFATNGIKRNQVKGPQGLA
                     LDAQGNLYVADTLNNRVLRFNGGVPGLGIEVALSGSGPGQVKLPGGLAVDAGFTLFIT
                     DVANSRILKLTNANTVPGSGNATPLATAGTGASQVRFPQGVAVDNAGNLYVADTGNSR
                     ILQFTGGQSGTGVILATVGSSLGQVRDAEGITVSAFTTGNLAGGSFLVIGDTNNNRIQ
                     GRFLAAGDWVLVGSPNGQGGNIGQFRTPSKIR"
     gene            1786..>2623
                     /locus_tag="PKZ53_12215"
     CDS             1786..>2623
                     /locus_tag="PKZ53_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008056917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNJ41249.1"
                     /translation="MTEKDSALLSELESLRKENARLKHRLAASQRSEVLLQAFLDTVP
                     FLVWISDRSGTCILQSQLALDQWGDLRGKTLADFEVPQDVASQWVDREQRVWAGEIIR
                     EEVFYTFQEQTRWVEEIIAPVKNNQEIIGLVGISLDISERKLFESELKHARDAALESS
                     RAKSEFLANMSHEIRTPMNAIIGLTGLLLDTHLTPDQLDFVETIRASGDNLLTIINDI
                     LDFSKVESGKLTLEMQPCNLRDCLEGALDLVATQALQKGLNLAYLIEESTPAVVMTDM
                     TRL"
BASE COUNT          599 a          677 c          710 g          637 t
ORIGIN      
        1 ccagccgcca cggtgaatgc cgggccagac cagaccatct gtgccgggag caccgtttcg
       61 ctggcaggtt ctctgggtgg cagcgccaca accgctacct ggagcggtgg cagcggtacc
      121 tattcgccag gttttggcgc tctcaatgcg acctacacac cgtcagccgc agaggttgcg
      181 gctgggtccg ttactttaac tctgactacg aatgatccag ccggaccctg cggaaccggt
      241 tctgacactg taactcccac gatcaacccg gcggcgggtg tcaatgccgg ccctgatcag
      301 gctgtatgtg ccagtaatgc ttctgtcatt ctggctggaa ttctaagcgg cagcgccact
      361 tccggcacct ggactggcgg cagtggtacg tttgtgccaa ataacaccac gctcaatgcc
      421 acctacaccc caacagcggc tgaaatccag gccggaaccg ttaccctgat tctgaccacc
      481 aatgatccag ccggagtgtg tggcgccaaa actgactcga tgaccatcag catcagtcca
      541 tcgtgtagcg aactggcggt gctgtatgtt gctgacacaa ccaacaatcg aatccagaaa
      601 tttgacggtc aagcctggag cctgatcggc ggtggtgctg gaagtgctgc cgtgggacag
      661 ttcaaacgac cagaagcggt tgtggccaat gttgctggaa cccgcattta tgtggctgat
      721 accggcaaca accggattca atggtcaact gatggtggag caacctggaa cctgtttgca
      781 accaacggca ttaagcgcaa tcaggtgaaa ggtccacaag gtctggcgct tgatgctcaa
      841 gggaatttgt atgtggctga tacgttaaac aaccgtgttc tgcggtttaa tggtggtgta
      901 cctgggcttg ggattgaagt tgctctgtct ggttctggcc ccgggcaggt caaattgccg
      961 ggaggactgg ctgtggatgc cgggttcact ctgttcatca ccgatgtggc caacagccgc
     1021 atcctcaagc tgaccaatgc caacaccgtc cccggatccg gaaatgcgac cccactggcc
     1081 accgctggaa ccggcgccag tcaggttcgg ttcccacaag gtgtggccgt tgataatgca
     1141 gggaacctgt atgtagccga cactggaaat tcacggattt tgcaatttac gggcggccaa
     1201 tcagggacag gcgtgattct ggccacggtg ggttcgtctc tgggtcaggt gcgcgatgcc
     1261 gaggggatca ctgtcagtgc ctttaccacc gggaatctgg ccggtggttc gttcctggta
     1321 attggcgata ccaacaataa tcggattcaa gggcgattcc tcgctgctgg tgattgggtt
     1381 ctggttggga gcccaaatgg acagggagga aatatcggtc aatttcgaac cccaagcaag
     1441 attcggtaat tttcttagaa cctgcggaat aagaagttgt tgaggatggg ttctaccaga
     1501 atctagcttt ctgcaagctt cttcatagtc tccaggccga accggtggtc aatttggact
     1561 ctgatgcctc agcgcttcag cgtctaaatt gatcaccggt ttttttcttt cccaaaagca
     1621 ggtagctcct ttcagatttg gagccgatgt tgtatttttg gccaacattg ctcgggtcat
     1681 aaatctatca gagttcattc tttatctttt ctggacacta ctttctccgt caccgtcaag
     1741 acacttgatt tgaggtttga accagggtgt tgacgaattt tggttatgac tgaaaaggat
     1801 tctgccctcc tgtcggaact tgaatctctg cggaaagaaa acgcacgtct caaacatcgg
     1861 ctggcggctt ctcaacgcag cgaggtcctg ttgcaggcat ttcttgatac tgtcccattt
     1921 ctggtctgga tttcagaccg gagcggcacc tgtattttgc aaagccagct tgcattggat
     1981 caatggggag acttaagggg caaaaccctg gcggactttg aggttccgca agacgttgcc
     2041 agtcaatggg ttgatcggga acagcgggtc tgggccggtg aaattattcg cgaggaagtg
     2101 ttttacacct ttcaagaaca gacccgatgg gttgaagaaa ttattgcccc agtcaaaaac
     2161 aaccaggaaa ttattgggct ggttgggatc tcgctcgaca tttccgaacg caaactcttt
     2221 gaatcagagt taaagcacgc ccgtgacgcc gccctggaat cttcacgagc caaatctgaa
     2281 tttctggcca atatgagcca tgaaatcagg actccaatga atgctatcat cggattaacc
     2341 gggttgttgc tcgataccca tctgacccca gaccagcttg attttgttga aaccatccgt
     2401 gccagtggcg acaacttatt aaccatcatc aatgatattc ttgatttctc aaaagttgag
     2461 tcgggcaagc tgacccttga aatgcagccc tgcaacctcc gggattgcct tgaaggtgcc
     2521 ctggacctgg tcgcaaccca ggcgttgcag aaagggctca accttgccta cctgattgaa
     2581 gaatcaaccc cggcggtggt gatgacagat atgacccggt tgc
//
LOCUS       DAOQDO010000741         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33624_length_2622_cov_4.362680, whole genome shotgun sequence.
ACCESSION   DAOQDO010000741 DAOQDO010000000
VERSION     DAOQDO010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33624_length_2622_cov_4.362680"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..831)
                     /locus_tag="PKZ53_12220"
     CDS             complement(448..831)
                     /locus_tag="PKZ53_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41250.1"
                     /translation="MRIPAMIRRRYSERGEGKGQFYITMAIMGIIGFALFKIVPVWYA
                     TTEFNNKVSELASVAALRGYKDQKIKDEFVKIRNEARMPEETEFIIEHKGNDLIVRVK
                     YTLHIDFIVYAYDWEENKSFTYGAV"
     gene            complement(1048..2199)
                     /gene="aroB"
                     /locus_tag="PKZ53_12225"
     CDS             complement(1048..2199)
                     /gene="aroB"
                     /locus_tag="PKZ53_12225"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNJ41251.1"
                     /translation="MTENSVVQVNLGERSYPVWVAANVCRQVGQMVTDRLGKYARTVV
                     IVSNPKVWGLYGPTVEASLQQAGIRTLVFLMGDGERFKTIQTATRIWSFLIEQKVERE
                     ASLLALGGGVVGDMTGFVAATYLRGIPYVQMPTTLLAQIDSSVGGKTAVNHPQGKNLI
                     GAFHQPALVLIDPVTLQTLPRREWNAGWCEALKYGVIRDASFFTELEQFLPFPTRRAS
                     IWTSEDMTFLSQAIARCCRIKADIVAADEREGGLRRILNFGHTFGHAFEAVTRYRWFR
                     HGEAVGYGMIAVCELAYQVGICSDETGNRVMNTILSLGHLPGLDPLNFDAVLLALQLD
                     KKSTGGTVRFILPRSIGDVIEVPSPERTVLAASLAAFCQKVKNWKLQAE"
     gene            complement(2196..>2622)
                     /locus_tag="PKZ53_12230"
     CDS             complement(2196..>2622)
                     /locus_tag="PKZ53_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41252.1"
                     /translation="LPQRILSPVRRAKMWELREAATCLVCAARYFHLTPGLPRPSLEQ
                     IASRFSELMQIEYTPSEALFFLNRGFEAGATVRAHCFEAQFRDYVKTRFGYWWRSRRA
                     GDVVIDLWNTGHRYTLEELAAQIGLGLVTAEALSEDWNA"
BASE COUNT          689 a          723 c          597 g          613 t
ORIGIN      
        1 atcgttcccg gcgctcaagc accggtctca attcagccac cgctacgcgg cgaaggaatg
       61 tcaccagacc ctcgcaagaa agagctggaa gtgaactgga ccaaccactc tgaaccagtt
      121 tcaccaggga cctgatgcaa atcaaaaaag tctccagcct gtttctcaaa ctcgtgatgg
      181 gtgcaaccct atgagagggt gctgatgcca ggttgcgata aaccttttgt gtcaggaagc
      241 agtcaagtca ttcaatcaca tacactttgc gagctactga ctccaaaccg atctgacctg
      301 accaccggga atttagggta cctgtacccg gtggtctttg gtcgtgaact taaaaacttc
      361 aactcaagga accgtgattt ttctgttacc tcagctcaga agagcacaat ccccaaaggg
      421 actgatcaca gtgaagtcgt accttttcta aaccgcccca tatgtaaatg acttattttc
      481 ctcccagtca taggcataaa caataaagtc aatatgcaag gtgtacttga ctctgacaat
      541 caggtcattt cctttatgtt caatgatgaa ctcagtttcc tcaggcatgc gggcctcgtt
      601 tcgaattttg acaaactcat ccttgatttt ttgatcttta tacccacgta aggcagccac
      661 actcgccagt tcactcactt tgttattaaa ttcagtcgtg gcataccaaa ctggaacaat
      721 tttgaagaga gcaaagccaa taatccccat gattgccatc gtgatgtaaa actggccctt
      781 tccttctcct cgctctgaat accgccgtct tatcattgct ggaatgcgca tgcccaagct
      841 cctttttcaa gttgaaattt cgaagaaggt cgtcttccct cgataagatc agaagacttc
      901 ccagaacccc tgaaagatcc gattgctttc agagttgtcc ctgattgatt tgagtgggtt
      961 ttgtgaatga agtactgagt ggtaactcga tagagaactc aatattggca taagttcagg
     1021 gtgactgcca gatgaaaaat aaagcggtca ctcagcctgt aacttccagt tcttcacttt
     1081 ttgacagaat gcagcaaggc tggcagcaag cacagtgcgt tcaggagaag gtacttcaat
     1141 cacatcaccg atactcctgg gcaaaataaa ccgaacagtg ccaccagttg attttttatc
     1201 aagttgaagt gccagcaaga ccgcgtcgaa attgagtgga tcaagccccg gcagatgtcc
     1261 cagactcagg atggtattca tcactcgatt ccctgtctca tccgagcata ttcccacctg
     1321 atatgccagc tcacagacag caatcatgcc atatccaacg gcttctccgt gacgaaacca
     1381 ccgatagcgg gtcacggctt caaaggcgtg gccgaaggta tggccgaagt tcaaaatccg
     1441 ccgcagtcca ccttcccgct catccgccgc cacaatatca gctttaatgc ggcagcatcg
     1501 ggcaatagcc tgactgagaa acgtcatatc ttcagaagtc cagatgctgg ctcgacgggt
     1561 tggaaacggg agaaattgct ccaactcggt aaaaaacgag gcatcccgaa tcaccccata
     1621 cttgagtgct tcacaccacc cggcgttcca ttcccgtctc gggagtgttt ggagggtgac
     1681 tggatcaatc aaaaccagtg ctggctgatg aaaggcccca atcaggttct tcccttgcgg
     1741 gtgattgact gcggtttttc caccgacaga actatcaatc tgggccagca atgtggttgg
     1801 catctggaca tagggaatcc cgcgcagata ggtggctgcc acaaaccccg tcatatcacc
     1861 cacgactccg ccaccgagtg cgagcagtga agcttcacgt tcgactttct gttcaatcag
     1921 aaaggaccag attcgggtgg cagtctgaat ggtcttaaag cgctccccat cccccatcaa
     1981 aaagaccagc gttcgaatac cagcttgctg gaggctggct tcaacggttg gaccatagag
     2041 tccccacact ttagggtttg aaacaatcac cactgttcgc gcatattttc caagccggtc
     2101 ggtaaccatc tgaccaacct ggcggcacac attggcagcc acccaaaccg ggtaggaccg
     2161 ttcccctaaa ttcacctgca ccacactatt ctcggtcatg cgttccaatc ctcactgaga
     2221 gcctcagccg tcaccagccc caacccaatc tgggcagcca gctcttccag ggtgtaccga
     2281 tgcccggtgt tccataaatc aatcaccaca tcgccagccc gacgggatcg ccaccagtag
     2341 ccaaaacggg ttttgacata atctctgaac tgcgcttcaa aacaatgggc tcgaacagtc
     2401 gccccggctt caaaaccccg attgaggaaa aagagcgcct cagatggggt gtattcaatc
     2461 tgcatcaact ctgaaaaacg gctggcgatt tgttcgagcg atgggcgggg gagtccaggt
     2521 gtcaaatgaa aatacctggc ggcacacacc aggcaggtgg cagcttcgcg gagctcccac
     2581 atctttgccc ggcgaactgg gctcagaatg cgttggggta at
//
LOCUS       DAOQDO010000742         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33655_length_2621_cov_3.095869, whole genome shotgun sequence.
ACCESSION   DAOQDO010000742 DAOQDO010000000
VERSION     DAOQDO010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33655_length_2621_cov_3.095869"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKZ53_12235"
     CDS             complement(<1..898)
                     /locus_tag="PKZ53_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41253.1"
                     /translation="MVLNLFSWLVLCQVLLTLSLSASAQTSQQPLPPAQQCEATQLTP
                     PASKIFNVQLKAGQFFRCVIEQGALDVAVVLYSPEGKQLTEVDSPNGGFGLEMVSWIA
                     DQDGTYRIEAKALDARAQPGKVCYRVVELREKSPADLTRLEIQSKLQEAIKSWNQQTP
                     ETQARAIEAGQAGFKLAQLIDDPTDRAQCLTYLGNFYLATNRQKLALEAYDQAVSEWE
                     KTTLSAELARLHPLLAQLYYVSRDSGKALTHATAAAQILTPHGDQDSLAQVLNILGNL
                     SADQGKNQEALVQYEQALAIYQK"
     gene            complement(1034..1879)
                     /locus_tag="PKZ53_12240"
     CDS             complement(1034..1879)
                     /locus_tag="PKZ53_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNJ41254.1"
                     /translation="MEQPAPLQTKPTPIPVSSQPTPSKNRPGAFLGELTWPDAEARLK
                     TSPVVIIPFGAGSKEHGPHLPMNADQKVMEYLIQQAVEHHPVLVAPPVLHGWFPGFRG
                     YPGTEVADPQVFQKYMFEIAMSLVKVGAKRIVFLNTGISLATGLPISQAAREIRVQTG
                     VPTLVLSWGDLETPELKALLKQKAGGHADELETSINLYLQPELVHMDRAVVDYTNSAD
                     KGYPGYKPGLYSRDPQDPQYSQTGMTGDPTLATAEKGKKALEIMTAQFMKALDGFSKE
                     PLKTN"
     gene            complement(1987..>2621)
                     /locus_tag="PKZ53_12245"
     CDS             complement(1987..>2621)
                     /locus_tag="PKZ53_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41255.1"
                     /translation="DILAAYGLRQAHAKATLSRSFAYATGPAYGCLLDSVNPVWRKQL
                     NTNRDLAHLAGTEFGIKLKQLDEPKLRAVATAYFGEELFEFESVRATRRRTQLAQIQS
                     QLIDGPTVELPLDETMNYSFDPNTVVTLDDNRTVYPSLQLSSAWGVLTASKGVMLCRE
                     KRKFSVALVSAHQIETGPAIEGDGWHLELKPGWRLEPAEKKASFRVVKHQ"
BASE COUNT          597 a          648 c          698 g          678 t
ORIGIN      
        1 gtttctgata gatggccagt gcctgttcat actgaaccag agcttcctga ttttttccct
       61 ggtcggctga aagatttccc agaatgttga ggacctgggc cagcgaatcc tgatcgccat
      121 gtggagttaa tatctgggcg gcggcggtcg catgggtcag agctttgcct gaatctcttg
      181 aaacataata cagttgtgcc agcaatgggt gcagacgggc caactcggcg gataaggttg
      241 ttttctccca ctctgaaaca gcctggtcat acgcctcaag ggccagtttt tgacggttgg
      301 tagcaaggta aaagttacca aggtaggtca aacactgagc ccgatcagtt ggatcatcaa
      361 tgagttgtgc gagtttgaat cctgcctgac cagcctcgat agctctggcc tgggtctctg
      421 gggtttgttg attccagctt ttgatggctt cctggagctt tgattggatt tcaagtcggg
      481 tcaaatcagc cggagatttc tcgcggagtt caaccactct gtagcaaact tttcctggtt
      541 gagccctggc atcgagggct ttggcttcaa tccggtaggt tccatcctgg tcggcaatcc
      601 agcttaccat ttcaaggcca aacccaccat ttgggctatc aacttcggtc agttgtttac
      661 cttccggtga atacagcacc accgcgacat ccagggcacc ttgttcgatc acacacctga
      721 aaaactgccc ggctttaagc tggacgttga agattttgga agctggtggc gtcaattgtg
      781 tcgcttcgca ctgctgtgcc ggggggagcg gttgctggct ggtttgagct gatgccgaaa
      841 gcgaaagggt caacaggacc tggcataaaa ccagccatga aaacaggttt aataccattt
      901 tgcaatgcag tccccgtatt accggaaggc taaagtattg aaaaaattgt atttccctga
      961 accctgaccc cgataccctg aaccctaatt ggtataaacc ccggaaatca gccattgtgc
     1021 ctcgtcatac agatcaattg gttttgagtg gctctttgga gaacccgtca agtgctttca
     1081 taaactgagc ggtcataatt tcaagcgctt ttttgccctt ttcagccgtg gccagagtgg
     1141 ggtctccggt catgccagtt tgtgaatact gtgggtcctg cggatcccga gaatacagcc
     1201 ctggtttata tccagggtag cctttgtcgg cagaattggt gtaatccacg acggctcgat
     1261 ccatgtgcac cagctcaggt tgcaaataga ggttaatcga agtctctagc tcatcggcgt
     1321 gccctccggc tttttgtttg agcaaggctt ttaattccgg agtttcaagg tcaccccagg
     1381 acaggaccag ggttggtacc ccggtctgaa cacggatttc acgcgcggcc tgggaaattg
     1441 gcagcccggt ggctagcgaa atgccagtgt tcaagaaaac aattcgtttg gcccccactt
     1501 tcaccagact cattgcaatt tcaaacatat acttttgaaa gacctgcggg tcagctactt
     1561 ctgtccctgg atatccgcga aacccaggaa accagccgtg cagcaccggc ggggcaacca
     1621 gcaccgggtg gtgttccaca gcctgctgaa tcagatattc cattaccttc tggtcagcat
     1681 tcatcggcaa gtgagggcca tgctctttcg atccggcgcc aaatggaatg ataacaacag
     1741 gtgatgtttt gagtctggct tcggcatcgg gccaggtgag ttcgccgagg aaggcgcctg
     1801 gccggttttt tgaaggcgtt ggttgactgg atacgggaat gggggttggt ttcgtttgga
     1861 gtggagcggg ttgttccata caaatgaagg ctgaactcaa aaagaagggg aggactgctt
     1921 gaagaattga cattatcaga ctccagctaa aataatttgg agagccgaat ccgatgttga
     1981 caggttttac tggtgtttca caaccctgaa acttgctttt ttctctgctg gttcgagtct
     2041 ccatcccggc ttgagttcca ggtgccaacc gtccccctca atggctggcc cggtctcaat
     2101 ctgatgtgcc gataccagtg ccaccgaaaa cttcctcttc tctcgacaca gcatcacccc
     2161 tttggaagcg gtgaggacgc cccaggctga cgatagctgc aacgatggat aaaccgtccg
     2221 attatcgtca agcgtcacca cggtatttgg atcaaacgag taattcatcg tttcatcaag
     2281 cgggagttcg accgttggac catcaatcag ttggctctga atttgtgcca gttgggtgcg
     2341 gcgtctggtc gcccgaaccg attcaaattc aaagagttct tcgccaaaat aggcagttgc
     2401 gacagcccgg agttttggct cgtcgagttg tttcaacttg attccaaact ctgtgcctgc
     2461 cagatgggcc agatcacgat tggtgttgag ttgttttcgc caaaccgggt taactgagtc
     2521 aagtaaacaa ccataggcgg gtccagtggc ataggcaaaa gatcgcgaaa gtgtagcttt
     2581 tgcgtgcgcc tgacgaagcc cataggcagc caggatgtcg g
//
LOCUS       DAOQDO010000743         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33706_length_2619_cov_3.466459, whole genome shotgun sequence.
ACCESSION   DAOQDO010000743 DAOQDO010000000
VERSION     DAOQDO010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33706_length_2619_cov_3.466459"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..2058
                     /locus_tag="PKZ53_12250"
     CDS             496..2058
                     /locus_tag="PKZ53_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41256.1"
                     /translation="MDEAEGASVSWPNLVGEATIHSVDLKPGQECLIISGYTDSPPKV
                     GDEIQITFPCYLRALHSAWSQSDWASLCLERLDQITRANQFDPETVLNPDGFAKLRTQ
                     QKTAFQLAGWSTSFLWGPPGTGKTTVTGALIGQFLVQFPHSRILVISNTNHAVDQLLV
                     MVDKALEHMSQTQTEVQAIRPRCIRIGTHFNPVSYKKREHLICRGKWQGSPLPKFDNP
                     FAQARLVALTTTRAIQSLNDLRQAGEFDWLVFDEAGQIGLSQAITLLPLAKKALFAGD
                     PRQLGPIHKARNPEAEVWLGTSVFDRVHFDQPWICFLDEQSRMASPICAVVSELFYGN
                     QLNLAADCEGSSMWHQGRQLDPALLFNRSVQQLSVTGSAVNSIRYGGYIRSESARVIS
                     QAVQDLKAAGLPEKHMVVVTPYRAQQKLIREYLRQKECQCVAVLTIHRAQGGEWHTVL
                     FDPVDGKNSFFHGKSACRLVNVAMSRAKARFLVTLSQTDCQNVILRELSVAIVEQEIL
                     AASRPADPAVAA"
     gene            2160..>2619
                     /locus_tag="PKZ53_12255"
     CDS             2160..>2619
                     /locus_tag="PKZ53_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41257.1"
                     /translation="MFFSFCGESGLPAVQYPEKWESHHQVELWRVSDDGPFKPLANHV
                     SNNGSPPNRGSSIAKTNRGGLVPADLGYDVAHASSSKFDKNLSNLSHPGRLLRVTEIH
                     MSFASCVQTFNSVENFLHRCGLTAKKVGGKYFISCPNPGHPDHNPSCTVSE"
BASE COUNT          706 a          647 c          587 g          679 t
ORIGIN      
        1 aactagccga acaaaaaggc attcggacaa tcaaggagct cgcagaccgt tctcacatgg
       61 cctatagcca ggtccacgct tactggcacg gtcacgttct tcaactcaat gtccgcaccc
      121 tccaacgcct tgccgaagct ctggaagttc gggtcaaaga cctctttatt gattaaccat
      181 tttcgtttcc aaattaaatt gacaaaatat agcactctag ctatagtact cttgtagtgt
      241 agttgcacga tgccttggta tcctgctgct ctgtcgtttg cctggtgcag gcattttttt
      301 tgctttgaat tatagtgctc tagctatagg gctatatcac ctgaggtcat catgattgaa
      361 aaacaaattc ttgccggtat tgaagcggaa atgaaaacta ctcctgaacc cctgaaggtt
      421 tcggccatcc ggtacatagt agaacaaaat tatttgaaaa tatttttcaa ccctcgaacc
      481 ctaaatcacc aaaagttgga tgaggctgaa ggagcatctg tttcgtggcc aaatttagtc
      541 ggtgaagcca ccatccattc agttgatcta aaaccaggac aggaatgtct cataatttct
      601 ggatacaccg actcaccacc taaggtagga gatgaaattc agatcacgtt tccctgttac
      661 ctccgggcat tacattcagc ctggtctcaa tcggattggg cttcattgtg tcttgaacgg
      721 ttagaccaaa taactcgtgc caaccagttt gaccctgaaa ctgtgctcaa cccagatgga
      781 tttgcaaaat tacgaaccca gcagaaaacc gcgttccaac tggccggttg gtcaacttcc
      841 ttcctttggg gaccgcccgg aacagggaaa acaactgtta ctggcgccct gattggacag
      901 tttttggtgc agttcccaca cagccgaatc ctggtaattt ccaataccaa ccatgcggtg
      961 gatcagctcc tggtcatggt tgataaagca ctcgaacata tgtcccaaac ccaaacagaa
     1021 gtacaggcca ttcgacctcg gtgtattcgg atcggaacgc atttcaaccc ggtatcttac
     1081 aaaaaacggg aacacttgat ttgtcgcggc aaatggcaag gcagtccgtt accaaaattt
     1141 gataacccat tcgctcaggc acggttagtc gctttgacta cgacacgggc cattcagtcc
     1201 ctgaatgact tacgccaagc tggtgaattt gactggttgg tgtttgatga agcagggcaa
     1261 attggcctgt cccaagcaat tacccttctc ccattggcca aaaaagctct atttgccggg
     1321 gatccgcggc aacttggtcc aattcataaa gccagaaatc ctgaagctga ggtttggctt
     1381 gggacttcag tatttgatcg agtccatttt gaccagccct ggatctgctt tttggatgag
     1441 caatcccgaa tggcctctcc gatctgcgcg gtcgtcagcg agttatttta tggaaaccaa
     1501 ttgaacctag cggctgattg cgaaggaagt agcatgtggc accagggacg ccaattggac
     1561 ccagcattgc tcttcaaccg atctgttcaa caactatcgg tcactggaag cgccgtcaac
     1621 tcaattcggt atggaggata tatccgttct gaatcagctc gggttatcag tcaagctgtc
     1681 caagatctca aagctgcagg actgcctgag aaacatatgg tggtggtaac gccgtatcgg
     1741 gcacagcaaa aattgatccg agaatatttg cgccagaaag aatgtcaatg tgtagccgtg
     1801 ctgacgattc atcgggccca gggcggggaa tggcacaccg tactgtttga tccagttgac
     1861 ggaaaaaata gtttctttca cgggaaatct gcctgccgcc tggtcaatgt cgcgatgagt
     1921 cgggccaaag ctcgttttct ggttactctc tcacaaacgg attgtcaaaa tgtaatattg
     1981 cgtgaattgt cagtggcaat tgtagaacaa gaaatactcg cagcatcgag accagccgat
     2041 cctgccgtag ccgcgtaatg ccataacttt ttgaacaaaa ataattaact caaaaaatca
     2101 cacacccttg atttttcatc agagaaagtt ttattttggg acccattagt tctcaaaata
     2161 tgttcttttc attttgcggt gaatcggggt tgccggctgt ccaatacccc gaaaaatggg
     2221 aaagccatca tcaggtagaa ctttggcgag tctctgatga tggccctttc aaaccgttag
     2281 caaaccacgt cagtaacaac ggtagccctc ccaatcgcgg gagttcaatc gcaaaaacta
     2341 accgtggtgg cttagtcccg gcggacttag gttatgacgt agcgcatgca tcgtcaagta
     2401 aatttgacaa aaatttgtca aacctaagtc atccaggacg cttgctaagg gtaacggaga
     2461 ttcacatgtc cttcgcctct tgcgtccaaa cgttcaattc agttgaaaac ttcctgcatc
     2521 gctgcggcct cacggctaag aaagtcggcg gcaaatattt catttcctgc cccaatcctg
     2581 gtcatcctga tcacaaccca agctgcacag tatctgaac
//
LOCUS       DAOQDO010000744         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33715_length_2619_cov_2.689158, whole genome shotgun sequence.
ACCESSION   DAOQDO010000744 DAOQDO010000000
VERSION     DAOQDO010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33715_length_2619_cov_2.689158"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2573)
                     /locus_tag="PKZ53_12260"
     CDS             complement(<1..2573)
                     /locus_tag="PKZ53_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ41258.1"
                     /translation="MKSRIAGTGLVAGSKVLFNQREVTGKVKPTTKKLIIKDLPADLL
                     AEDRTYEVAVVTPEGLRSNTVSFKVGTGIGRIPSDIKITEPDSLLINANVSTQLRVKV
                     LDGNGVEIPNPSLTFETEDQTLASITADGRLTALGPGVAAIRIRSGELSKTFSFQISS
                     VATVSSGIFAESDMVVQGTTILASDFANHVIRRSQAFQPMSDFAGRSGLSGDADGAGD
                     SARFNAPLGIGIRNGNVYLVDTANRAIRQVDVASGAVKTILTEADLATAGLATSWAPR
                     DIEIDGNEDLFITDAANHVIWQASPKGSGYRLSLLAGTLGQAGTQDGTGVSAQFDTPQ
                     RLTLVGRILSVTQATDQIRQIALPGGAVKTIQSQAGRQLRNLPLTDPGGITADADGNL
                     YVTVPGNVNVVTIMDEQVTTRDLLPPGTVIQPVAVALDETSFYILDRANGQLVRATFG
                     KPAITTLAPTQINAGSGSTEITIQGKNFSPETQVFAGNEPISNVNVLNPTRLQFTLPP
                     QLFGESVTIRVRTPGGVATTTLNITGPNRPGGVSLEGYPENRSLKPGEQTTFTINLQR
                     VNFTGSVLLSVSGLPNGVTAQFLPNPAVAETAKLVLTASQTVSGGTFPLQVRGTASGV
                     TVQSAVLSLGVNVDARVILGATPTFQTVTQGKGTFFNISLSRTNYTGTVNFSVTGAPA
                     GSNPFFTLNEITGNSTSLRLELRPDVVPGTYQLKIGGTGAGEINVVPVVVTMRVLPIQ
                     PSVRIIIEPPQWSVLAGQGTTYIAILSRTEYLLPVRLTLEQIGGSQDAGVKAILGAND
                     TTAFRFPVLVTSTPETPPGAYRFRITGKADGAIVYDSNDFVFNVVPPPPIVQLGVDPL
                     SR"
BASE COUNT          624 a          690 c          684 g          621 t
ORIGIN      
        1 cgacttaacg ggtctacgcc taactgaaca attggcgggg gcggaacaac attaaataca
       61 aaatcatttg aatcataaac aattgcacca tcagcctttc cggtaatccg aaaccgatag
      121 gcgccaggcg gagtttcagg agttgatgtc acaaggactg gaaaccggaa ggcagtggtg
      181 tcattggctc caagaatggc tttcactccg gcgtcctggc ttcctccaat ttgctcaagc
      241 gtcaatcgaa ctggcaacaa atattcagtt cgactcaaga tcgcaatata ggtggttccc
      301 tgtccggcca ggaccgacca ttgcggcggt tcaatgataa tcctgacgct tggttggatc
      361 ggcaataccc gcatggtcac aaccactggc accacattga tttcgccagc gcccgttcct
      421 ccgattttaa gctgataggt gccaggtacg acatctgggc gcaactcaag ccggagcgac
      481 gtggaattgc cagtgatttc gttgagcgtg aagaatggat ttgaacctgc cggtgcccca
      541 gttaccgaga aattgaccgt gccggtgtag tttgtccggc tgagcgaaat attgaaaaat
      601 gtgcccttcc cctgagttac agtttgaaaa gttggtgtgg cgcctaaaat caccctggcg
      661 tccacattga ccccaagtga aagcacggct gattgaaccg ttaccccaga cgcggtgcca
      721 cgaacttgca agggaaaggt cccgccagaa acagtttgtg aagctgtcaa caccagcttc
      781 gccgtttcag ccactgccgg gttgggcaaa aattgagcgg tcacgccgtt tggtaagcca
      841 ctgacagaaa gcaacacact accagtgaaa ttgactcgct ggaggttaat ggtaaaggtt
      901 gtctgttccc ctggtttgag tgaccggttt tccggatacc cttcaagtga aacaccacct
      961 ggccggtttg gtccggtgat attgagagtg gttgtggcga caccgcctgg ggttcggacc
     1021 cgaatcgtca ccgattcacc aaagagttgc ggaggaaggg taaattgaag ccgggttgga
     1081 ttgagtacat tgacgtttga aatcggctca tttccagcaa aaacctgggt ctcgggcgag
     1141 aaattttttc cctggatggt gatttcggta ctcccacttc cggcattgat ctgggttggc
     1201 gcgagcgtgg tgatagctgg ttttccaaaa gttgcccgaa cgagctgccc attggcacga
     1261 tccaggatat aaaaactggt ctcatccagc gcaaccgcaa ccggctggat cacagttccg
     1321 ggtggcagca aatcccgcgt tgtcacctgt tcatccataa tggtcacgac gttgacgttc
     1381 cctggaaccg tcacatacag atttccatcc gcatcagccg taatgccgcc cggatcggtt
     1441 aacgggaggt tgcgtaattg acgaccggcc tgtgattgaa ttgttttgac agctccaccg
     1501 ggcaaggcga tctggcggat ttggtcggtg gcctgggtga ctgacagaat tctgcccacc
     1561 agagtaagcc gttgaggggt gtcaaactga gcggaaacac cagttccatc ctgtgttcca
     1621 gcctgcccta aagtcccagc cagcaaactg agacgatagc cactgccttt gggacttgcc
     1681 tgccagatga catggttagc ggcatcagtg atgaaaagat cttcgtttcc gtcaatttca
     1741 atatcacgcg gcgcccaact ggttgccagt ccagcagttg ccaggtcggc ttcggtgaga
     1801 atggttttta cagcgcccga agcaacgtca acctggcgaa tcgcacgatt ggcggtatcc
     1861 accagataaa catttccatt gcggatccca attccaagcg gtgcattaaa tcgggcagag
     1921 tcacctgctc catcagcatc acccgagagt cctgaccgcc cggcaaaatc gctcatcggt
     1981 tgaaaggcct gactacgccg aatgacgtga ttggcaaaat cagaggccaa aatggtggtt
     2041 ccctgcacca ccatatcgct ctcggcaaaa atccctgacg atacagttgc caccgaactg
     2101 atttggaatg aaaaggtctt gctcaattcc ccagagcgaa tgcgaatcgc cgccacgccg
     2161 gggccgagag ccgtcagccg tccgtcagcc gtgatgctgg ccagtgtctg gtcttcggtt
     2221 tcaaacgtca gtgatggatt tggaatttca acgccattgc catccagcac cttgacccga
     2281 agttgggtag atacattggc attgatgagg agcgaatctg gctcggtaat tttaatatca
     2341 gacggaatcc tgccaattcc cgtgccaact ttaaagctaa ccgtgtttga tcggagtccc
     2401 tctggggtga ccacagccac ctcataggtc cggtcttcgg ccagcaaatc agccggaagg
     2461 tctttgataa tcaacttttt ggtggtgggt tttaccttgc cggtgacttc acgctggtta
     2521 aaaagtactt tcgaaccagc caccaaacca gtcccggcaa tccgactttt catcgttcag
     2581 tttctcgtat tgcagaatgg cttcttccgc cattttttg
//
LOCUS       DAOQDO010000745         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33728_length_2618_cov_3.219274, whole genome shotgun sequence.
ACCESSION   DAOQDO010000745 DAOQDO010000000
VERSION     DAOQDO010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33728_length_2618_cov_3.219274"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1536)
                     /locus_tag="PKZ53_12265"
     CDS             complement(139..1536)
                     /locus_tag="PKZ53_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNJ41259.1"
                     /translation="MTPQPSSASVSSPKQRTKGGVHAPVAFDADLYKARRAQVLERLG
                     RNTLVLFSPPEAHRTHDLNYRYRPDSDVYYLTGFDEPETVVVLTNDHPEHKFVMFVRP
                     RDAERETWDGFRAGLEGAVSRYGADVAFPISEFSSKIADYLNNGQTLYYRFSRDEVFN
                     QHIFNAMKAARWRSHRSGHAPDTIRDTGCLLGEMRLRKDPSELNRMRVAGEIAAEAHC
                     EAMLAAAPGKYEFEIEAVIEYVFRRRGALGPAYTSIIGSGANATILHYNTNDRLMEDG
                     DLLLIDAGCEFGYYASDITRTFPVNGKFTDPQRKIYEAVLKSQLASIDMVKPGNRFID
                     YHNKATEVLVEELLKLGLLEGTVEKNLEDKAYMKFYMHRAGHWLGSDVHDACSYVVEQ
                     EDDFVSRIFEPGMVVTVEPGLYFQTSLEGVPAEYLGIGIRIEDDVVVTETGNEILTAG
                     VPKFIDEIEALMARG"
     gene            1818..>2618
                     /locus_tag="PKZ53_12270"
     CDS             1818..>2618
                     /locus_tag="PKZ53_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ41260.1"
                     /translation="MVQQLVTGGAGFIGSHLVEALLGRGYQVRVVDSLITGHKENLAG
                     FLSDIEFIEGDLADPEVAARAVQGVECVFHEAAIPSVPRSVRNPLQTQRSGEISTLNL
                     LDAAVRADVRRVIFAASSSAYGNTEVLPKVETMPPRPLSPYAASKIAGEGYMSAFAAC
                     HDLDTVSLRYFNVFGPRQDPTSQYSGVIAKFTDIMPRGIRPVIFGDGEQSRDFCFVKN
                     VVHANLLAMDCPERLGGEVMNIGCGGRITLNELVAILNRIFGTDHTPIY"
BASE COUNT          593 a          669 c          699 g          657 t
ORIGIN      
        1 gaagacatcg ggctcagggc ttggggctga agactcgcaa gctcggggct gaagaaaatg
       61 ggttcaatac cctgaaccca ttttcttcag cccactttct tcagcccgat gtcttcagcc
      121 cccagccctg agccctggtt atcctcgggc catcagggct tcaatttcat caataaactt
      181 cggaacaccc gccgtcagga tctcattgcc agtttcagtg accacgacat catcttcgat
      241 acggatgccg attccgagat attcggctgg aacaccttcg agtgaggttt ggaagtagag
      301 ccctggttca accgtgacca ccattcccgg ctcaaagatg cgcgacacaa aatcgtcttc
      361 ttgttcgacc acatagctgc aggcatcgtg cacatcgctt ccaagccagt gcccggcgcg
      421 gtgcatatag aacttcatat aggctttgtc ttccagattt ttctcaaccg tgccttccag
      481 caagccgagt ttgagcaatt cttcaaccag tacttcggtg gccttgttgt gatagtcaat
      541 aaagcggttt cctggtttga ccatgtcaat tgaagccagt tgggatttca aaacggcttc
      601 ataaatcttg cgctgtggat cggtaaattt cccattgacc gggaacgttc gggtgatgtc
      661 tgaagcgtag tagccaaact cacaaccggc atcaattagc aacaagtcgc catcttccat
      721 gagccgatca ttggtgttgt agtgcaaaat cgtagcattg gcgcctgacc caatgatgct
      781 tgtataggct ggccccagcg cgccacgccg tcgaaagaca tattcaatca cggcttcgat
      841 ttcaaattca tatttgcctg gggccgcggc cagcatggcc tcgcagtgag cttcagcggc
      901 aatttcgccg gcaactcgca tccgattcag ttccgatgga tcttttcgga ggcgcatttc
      961 gccaagcagg catccggtgt cgcgaatcgt atctggcgca tgtccgctcc gatgtgatcg
     1021 ccatcgggca gctttcatgg cgttgaaaat atgctggttg aagacttcat cgcggctaaa
     1081 ccggtagtac agagtctggc cgttgttgag gtagtcggca attttcgatg aaaactcact
     1141 gatcggaaag gcgacatccg ccccatatcg gctcaccgcg ccttccagcc cggcccggaa
     1201 gccatcccag gtttcgcgtt ccgcgtcgcg tggtctgaca aacatcacga atttgtgttc
     1261 cggatgatca ttggttagca ccacgacggt ttctggctca tcaaatccgg tcagataata
     1321 cacatcgctg tccggacgat agcgataatt caaatcgtgg gtgcggtggg cttcaggtgg
     1381 gctaaaaagc accagcgtat tccggcctaa ccgttccaaa acctgcgccc ggcgggcttt
     1441 gtacaggtcg gcatcaaatg caaccggagc gtgaactccg cctttcgtgc gttgtttggg
     1501 tgaagagaca cttgcgctgg atggttgtgg cgtcatgcaa tacctcgcgt gatctggatt
     1561 aatatagaaa tgaaggattc cccactatac cattggcctt gggaaaacgg ctacctggga
     1621 aaagtttctg gttggaaagg tctttggaaa aggaattgcc tttgctgata gcattgtaac
     1681 cgggctaagg gttgctggcg cttggtacct ggtgactggt atttagtacc tggtgcttgg
     1741 gatccaatac cttccaagaa ccaaaaacta agcaccaaga accagaaaac tcgaaaaagg
     1801 agaaagcaga aatccatatg gtacaacaac ttgtgacggg cggagcgggg tttattggga
     1861 gtcatcttgt cgaggcattg ctggggcgtg ggtatcaggt acgggtggtg gattcgctca
     1921 tcaccggcca caaggaaaat ctggctggat ttctgagcga tattgagttt atcgaaggcg
     1981 atttagctga tccggaagtt gccgcccggg ctgttcaggg ggtggaatgt gtgtttcacg
     2041 aagcggcaat tcccagcgtt ccgcgttcgg tccgcaaccc gctccagacc cagcggtcag
     2101 gtgaaatttc cacgttgaat ctgcttgatg ctgccgtgcg ggccgatgtg cgacgggtga
     2161 tttttgcggc ttcctccagt gcctatggca acaccgaagt gttacccaaa gtcgagacca
     2221 tgccgccgcg tcctttgagt ccgtatgccg ccagcaaaat tgccggagaa ggatacatgt
     2281 cagcttttgc ggcatgtcac gatctcgaca cggtttcgct tcgctatttc aatgtctttg
     2341 ggccgaggca agatcctacc agtcagtatt caggggtgat tgccaaattt accgacatca
     2401 tgccgcgtgg cattcgtcca gtgattttcg gagacggcga acaatcccgt gatttctgct
     2461 ttgtcaaaaa cgtggttcac gccaatttgc tcgccatgga ttgccccgag cggttaggtg
     2521 gagaagtgat gaacattggc tgcggtggcc gaattactct gaatgaactg gttgctattt
     2581 tgaatcggat atttggcacc gatcacacac cgatttat
//
LOCUS       DAOQDO010000746         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33750_length_2617_cov_3.322404, whole genome shotgun sequence.
ACCESSION   DAOQDO010000746 DAOQDO010000000
VERSION     DAOQDO010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33750_length_2617_cov_3.322404"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKZ53_12275"
     CDS             complement(<1..285)
                     /locus_tag="PKZ53_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41261.1"
                     /translation="MVCSTCGATLQPDQMVCGICGKPNAKLPTSILGSRPNATEPLEY
                     FFLSQSGTANGALPTAVSRTAAPLQGETLVKDIPNPTTALPPTSHIQVCRR"
     gene            complement(579..1049)
                     /gene="greA"
                     /locus_tag="PKZ53_12280"
     CDS             complement(579..1049)
                     /gene="greA"
                     /locus_tag="PKZ53_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNJ41262.1"
                     /translation="MKVKLILEEEIRKLEQELKIELPRELQRAAALGDLRENGEYQAA
                     RERQRYVQARLGALHQRLAALSMLNIDQLPRDRAAYGSTLEVLDLDRDTEITYRLVTP
                     EEVDVPNGLISTTSPLGRCFMGKEEGDEVQAQTPQGVRRFEILKLRTIHDELGA"
     gene            1681..>2617
                     /locus_tag="PKZ53_12285"
     CDS             1681..>2617
                     /locus_tag="PKZ53_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41263.1"
                     /translation="MKIRDFVDAFRRKPANQESSVPPKNKGEDEPLPLVEVADVVSKA
                     ERPPVPILSAPVVQTLMGSVPIPQRFDPRRGRDSALSSAGMVSQPVPLEPSGVPPAVP
                     DPEPDASNILEIGLEPGPAVSLEQMDTEPTLGQSASWAEADPLGDLVPTAPLPATPLV
                     PPPPPPSPLAASLADPLADFHTPPTPTPSPSTQHMANPFASPPPSPPPAPSSPPPRMV
                     PPAPPLADPWFSSPPPPPPLSPPSRPTPVGEMTGLDALLSSSSEGIPTFKPVSSLPPA
                     ARPTPVRQPALDTRLIEVFQKLAIFTGDLAETTSSE"
BASE COUNT          592 a          732 c          688 g          605 t
ORIGIN      
        1 gcgccgacaa acctggatgt gtgatgttgg tggcaatgcg gtggtggggt ttggtatgtc
       61 tttgaccaga gtttctccct gaagcggtgc tgccgttcgg gaaaccgctg tgggcagggc
      121 gccgttggct gtgcctgatt gggaaagaaa aaaatactcc aacggttcag ttgcatttgg
      181 gcgagaaccc aggatggatg tcgggagttt ggcattgggc tttccgcata tgccgcacac
      241 catctgatcc ggctggagcg tggctccgca cgtagaacac accataaaat ttcctcactt
      301 gagcaaaaaa actgtgtcgg ggatttgagc caattgccgt gaccatcagc attgaggcca
      361 tggccacgtg aagtacggga ggcttgacag ttttccagat gaggaaatcc aaatgagcgt
      421 tggttgtacc gtaaggaaat aaaccaggag ggaatggtgt caaagcgggc agtttcctca
      481 gagaaaagcc agtgagccag ggcagttggg gaaagggaag tcttgattct ttacccgttc
      541 aagcccccaa ttcccaatct ggtcactgac ttaccctgtt atgctccgag ttcgtcgtga
      601 attgtccgca acttgaggat ttcaaaccgc cgcacgccct gtggggtttg ggcctgcacc
      661 tcatcacctt cttcttttcc cataaagcac cgacccagag gagaagtcgt tgaaatcaaa
      721 ccatttggca catcaacctc ttcgggagtg acgagccggt aggtgatttc tgtatcacgg
      781 tccaggtcga gaacttcaag cgttgatcca taggcggccc gatcacgggg taattgatca
      841 atattgagca tggacaacgc ggccaggcgt tgatgcaggg cgcccagacg ggcctgaaca
      901 tagcgttgcc gttcccgagc cgcctggtac tctccgtttt cgcgcaagtc accgagtgcg
      961 gcagcccgct ggagttcgcg agggagttcg attttgagct cttgctcaag tttgcggatt
     1021 tcctcttcca gtatgagttt tactttcatt caatcaaagg cgtggatccc cagcgtgtga
     1081 tactgagtgg ggccgcctga ctcctttcaa agataatttc ccacgagact caatcttctc
     1141 aaacatgacg tactcggacg aggggttgat ggggaatccg gttttcagaa aaaaagaaac
     1201 cccggtcagg ccaaatcgaa cctgactggt gtgacgaaac ctctcggacg ttggtggatg
     1261 aacgaagaat cctgaatctt tcaggggaga gagtttctgg cattttttcc ctaaaaaatc
     1321 aagatgcctt ggataagaaa ccgagacttt gcacagtggg agtcttgtcg cctggagatg
     1381 ctttagacta tactttcaac tcgtcggttc tggctaaaac acttcgtagc accatttaca
     1441 tttcgttaac ccagcacaac acgtcccgaa aaagagttta tccggataag tcttttttct
     1501 gtaccactat acgcttcaga aagaagccac cggtaagccc tgattttcag gaaactcctg
     1561 tcgcaacagg aggatttgtg gcactggcac cccaccgatt tcactgtgaa ttcgcttgtg
     1621 gatgctggtc atgggtctgg tagggcatcg gctcagatct tcggaatcat cagtgacatc
     1681 atgaaaatac gcgatttcgt tgacgctttt cggcgcaagc ctgccaatca agaaagttcg
     1741 gtgcctccta aaaacaaagg ggaggacgaa ccgcttccgc tggttgaagt ggcagatgtt
     1801 gtctcgaagg ctgaacgacc cccggtacca attctatccg ctcccgtggt ccaaacactc
     1861 atgggatccg tgccgattcc acaacgattt gacccacgtc gagggcgaga ctccgctctt
     1921 tcatctgcgg ggatggtgag tcagcctgtc cccctggaac caagtggggt gccaccagca
     1981 gtaccggatc cagagccaga tgcctcaaat attttagaga tcggattaga gccaggccct
     2041 gcggtgagct tagaacagat ggatactgag ccaactctgg ggcaatctgc cagttgggcc
     2101 gaagctgacc cactgggaga cctggtccca acggcccccc ttcctgccac accattggtc
     2161 ccaccgccgc caccgccatc accactcgcc gcatcactgg ctgatccgct ggctgatttt
     2221 cacacaccgc caaccccaac cccttcacca tcaacccagc acatggcaaa cccctttgcc
     2281 agcccgccgc cgtcgccacc gcccgcccca tcttcaccgc cgcccaggat ggtacccccg
     2341 gcgccgcctc tggctgaccc ctggttttcc tcgccaccgc cgccaccgcc actgtcaccg
     2401 ccatcacggc caacgccagt cggggaaatg accggattag atgctttgct gagttcttca
     2461 tccgaaggga ttccgacttt taaacctgtg tcgtcgttgc cgccggctgc ccgtcccacc
     2521 ccggttcgtc aaccggctct ggatacgcgg ttgatcgagg tttttcagaa actggccatt
     2581 tttacaggag acctggctga aacgacctct tcggagg
//
LOCUS       DAOQDO010000747         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33832_length_2614_cov_2.892927, whole genome shotgun sequence.
ACCESSION   DAOQDO010000747 DAOQDO010000000
VERSION     DAOQDO010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33832_length_2614_cov_2.892927"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKZ53_12290"
     CDS             complement(<1..811)
                     /locus_tag="PKZ53_12290"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HNJ41264.1"
                     /translation="MFGLMKARTCAQPPAQRREHRLHYCGTCKTIGRQYGQSARFLLN
                     FDTVFLAEVLTALGDQPASLEPWAPAYQSFNCLTLPRTVDEFPLPLHFAATATVVLAE
                     FKVQDHLQDNSSWSGRVAATTFSTAFQTAAQQLASWAFPVDELRQTLGSQTQREGELK
                     QAGSATDPEAGLSLVAEPTAHATGLFFRHGARLLGATEAESTMYSLGQAFGTLIYLLD
                     ALEDFEKDQRRGDFNAFQRVFAIDQPKLTGPHRAQAIAQVRNAEARMIEFFH"
     gene            complement(891..1619)
                     /locus_tag="PKZ53_12295"
     CDS             complement(891..1619)
                     /locus_tag="PKZ53_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41265.1"
                     /translation="MSVQRSPQVSLYYEDLTIGEMLKTMINQHPLPVFGMVLGLLVLA
                     GGLGFEVGRNLVSNQGGKPVGTQNTASDSGQLRQDLTQCQTELSNLKASSSAQIAVLQ
                     RKVETEAKAMQMRYEYDLDFLNRRVDEYREIVGRTPEARKREAFLNLLISYQTATDFA
                     RRQESPQTVQNRERTIEELQKFVLTNLKLTPTERTLFDFSLMAEPNGATYRVTAQAGN
                     SRRLIPAEVIDPIAAQIRANLPKK"
     gene            complement(1818..2498)
                     /locus_tag="PKZ53_12300"
     CDS             complement(1818..2498)
                     /locus_tag="PKZ53_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ41266.1"
                     /translation="MRRVLIVEDDPSITVALRDGFEYEGYSVLIARDGVEGLRMATEK
                     EIDLVILDVMLPKMSGFDVCKQLRAASNSTPIIMLTARGQEIDKVLGLKMGADDYITK
                     PFSFMELMARVEAVLRRVSRQNTQADFYEFGDITLDLKKYEARKKGEVLDLSPREIKI
                     LKYFIDHRGEVVTRDHLLDVVWGYNSAPLTRTVDMHIAKLRQKIEDTPGDPKYIITVH
                     RVGYKFTS"
BASE COUNT          618 a          647 c          688 g          661 t
ORIGIN      
        1 ggtggaagaa ctcaatcatt cgggcttcgg cgtttcgcac ctgagctatg gcctgggccc
       61 gatgaggtcc ggttaatttc ggttgatcaa tcgcaaagac cctttgaaag gcgttgaagt
      121 caccccggcg ctggtctttt tcaaaatctt caagtgcatc aagcagatag atgagcgttc
      181 caaacgcctg acccagactg tacatcgtgg attcggcttc ggtggcgccg agcaaccgtg
      241 cgccgtgacg aaaaaagagg cccgtggcgt gggccgtggg ttcggccacc agggaaagcc
      301 cggcttcggg gtcagttgca ctgccggctt gtttgagttc gccttcacgt tgtgtctggc
      361 ttccaagtgt ctggcgaagc tcatcaaccg gaaatgccca ggaggcaagc tgttgagcgg
      421 cagtctgaaa cgcagttgaa aaggtcgtgg cggccactcg accagaccac gatgaattgt
      481 cctgaaggtg atcctggact ttaaattctg ccaaaaccac ggtggcggtg gccgcaaaat
      541 gaagtggtag cgggaattca tctacggtgc gcggcagggt cagacaatta aacgattgat
      601 aggccggcgc ccacggctca agcgaagcag gttggtcacc cagagccgtc aacacttcgg
      661 ccagaaatac cgtgtcgaaa tttaaaagga agcgggctga ttgcccatac tgacggccaa
      721 tcgttttaca cgtgccgcaa tagtggagtc ggtgttcgcg gcgttgagcg gggggctgag
      781 cacaggttcg tgctttcatc aatccgaaca tgattcactc ccgtggagtg cggtggcttg
      841 acaccgcttt cgatatggag tgcggtggct tgacaccgct ttcgatcttg tcacttcttc
      901 ggcaggttgg cccggatctg agccgcaatc gggtcaatca cttcggcggg gatcagtcgc
      961 cgtgaattac cggcctgggc tgtcacccgg taagtggcgc cattgggttc ggccattaag
     1021 gagaaatcaa acaacgtacg ctcggttggg gtcaatttca ggttggtgag gacaaatttc
     1081 tgcagctctt ctattgttcg ctctcgattt tgcactgtct gtggagattc ctgccggcgg
     1141 gcaaaatcag tggcggtctg gtaggaaatc aaaaggttta aaaaggcttc ccgttttcga
     1201 gcttcagggg tccgaccgac aatttcccga tactcatcaa cccggcgatt caaaaagtcg
     1261 aggtcatatt cataccgcat ctgcatggct ttggcttcgg tctcaacttt gcgctgcaaa
     1321 accgcaattt gggcagatga actggctttg agattgctta attccgtctg acattgggtc
     1381 aggtcctgtc gaagttgtcc agaatcactg gcagtgtttt gcgttccaac tggttttccc
     1441 ccttgattgc ttaccagatt gcgtcccacc tcaaacccca gtcccccagc cagaaccagc
     1501 aaaccaagaa ccataccgaa gaccggcagt ggatgctggt ttatcatcgt tttcaacatt
     1561 tcaccgatag ttaaatcttc atagtacaaa ctgacttgcg gagatcgttg gacactcata
     1621 ttgggggtta agcgctcctt aatcgtaaag ttgcggcctg gactaacaat agccgagaag
     1681 ccgattggcg acaattagca gttcgacaaa aaatctctac ttcaaaagcc tttgactgga
     1741 aaaacaaatg gcggaagcgt tgttgcttcc gccatttccg ctcaatctgt tttcgaaatc
     1801 ggagttgagg ttgtcgctta gctggtgaat ttatacccga ctcgatgaac cgtgatgatg
     1861 tattttggat cacctggggt atcttcgatt ttttgacgaa gcttggcgat atgcatatcc
     1921 acggtgcgcg tgagaggggc gctgttgtat ccccaaacga catcgagcaa gtggtcacgg
     1981 gtgacaactt cgccacgatg atcaataaag tacttcaaaa ttttgatttc tcgcggagac
     2041 aggtcaagca cttcgccttt tttgcgagcc tcgtacttct tcaaatccag cgtaatgtca
     2101 ccgaattcat aaaagtcagc ctgggtgttt tgacgtgaaa cacgacgcag aacggcttcg
     2161 actcgggcca tcaactccat aaaactaaag ggtttggtaa tgtagtcatc agctcccatt
     2221 ttcaagccca gcactttgtc aatttcctgc cctctggccg ttaacataat gatgggggta
     2281 gagttgctgg cggcacgaag ctgtttgcaa acgtcaaaac cgctcatctt gggtaacatc
     2341 acatcgagga tgaccagatc tatttccttt tcggttgcca tccgtaagcc ttcgacgcca
     2401 tcccgggcaa tcaggacgga atacccttca tattcaaacc catcccgtaa ggcgacggtg
     2461 atggagggat catcttctac gatcaagact cttcgcataa taaacacgtg ggggcagtgg
     2521 aatgattttt tgttgcagtg tggttgagaa ttgtgaacat gcctagtacc tggttcaagt
     2581 caaaaaagtt ttcaaattct ccgcctgaaa cctc
//
LOCUS       DAOQDO010000748         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33835_length_2614_cov_2.786635, whole genome shotgun sequence.
ACCESSION   DAOQDO010000748 DAOQDO010000000
VERSION     DAOQDO010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33835_length_2614_cov_2.786635"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKZ53_12305"
     CDS             <1..655
                     /locus_tag="PKZ53_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41267.1"
                     /translation="VRVFVFSIDTDANLANAQINNVKQADFTITKDFSDPAQITSYSN
                     YNEFDTPDFTIDRGSFVVLIKLISTGSSQLSRDGRDFFFMADIRQPSKAVISPDDGRS
                     LTGVGVQVNNLQVDFPVLARVTLANTSVPPPSIEKVRFAKNKLSVFGKQLSLTGQLRI
                     NNKVVVPPLLARYVQETGRLRVQATQQQLNLKEGENEISFTEGKLTSASFRFKYSPK"
     gene            834..>2614
                     /locus_tag="PKZ53_12310"
     CDS             834..>2614
                     /locus_tag="PKZ53_12310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HNJ41268.1"
                     /translation="MKQILIPFLVACFLAPGSMWVSSVSPVSQISAVHIPQRQVPGGL
                     PFVADLVKTQARVPAFRHIGPLTVVLASRDPKGLQDLLAAQQNPASPEYHQWLTPQEF
                     GNRFGRLPQEYAQLQEWLRSTGLTVSQQWPNRLQLTVEGPAEAVERAFDIQLDEYQLN
                     AEVGFAANRMPSVPAIFPFIKGISGLHSFTRFQPMVSETSLTPGVAHTGNTFSHRGPS
                     NLMAPADIATVYNLKPLLDQGLDGSGVTIAVAARCDFNLSDPVAFRSQFNLPPKDPIK
                     VFPFGPRPNRGGVEEVEAVLDVEWAGATAPDATVKSVIAPDIDFSIQAITNNMPEAKV
                     ISVSFGGCERFNGLEVAEFIEMLFAQAATQGQSILVSSGDNGVADCLRIDGTTAPSVN
                     VLASPPQATGVGGTNVDPQFDTNGNATRNGGETGWTGSGGGDSIFFPKPAFQTGVGVP
                     DTTVRTVPDVALLAGSPAYAYIIRGALAGVGGTSVSTPCWAGLCAILNEARKTNGLGN
                     INPEIYRLGNAQATGAGAQVFFDTLTGNNSFGAVQGFTCQAGYDRVTGWGSYNAALLV
                     ENFRAQPVTDTVAPTISLQSPVGGEEIL"
BASE COUNT          607 a          711 c          658 g          638 t
ORIGIN      
        1 ggttcgagtc tttgtttttt caatcgacac agatgccaat ctggccaatg ctcaaattaa
       61 caatgtcaag caggctgact ttacaatcac gaaggatttc tctgacccgg cccagattac
      121 gtcttattca aactataatg agtttgacac cccggatttc acgattgacc gggggagttt
      181 tgttgttctc atcaaactga tttcaactgg ctcaagccag ctcagccgtg atgggagaga
      241 ttttttcttt atggctgata tccgtcagcc aagcaaagcg gtgatctcgc ctgacgatgg
      301 ccgcagcctg actggggttg gcgtccaggt caataatctg caggttgatt ttccggtctt
      361 agcccgagtc accctggcca acaccagcgt tccgccacca tctatcgaga aagtgcgctt
      421 cgccaaaaat aaactttccg tcttcggaaa acagctttcg ctcaccggcc agcttagaat
      481 taataataaa gtggtggttc cgccattgct cgcccgctat gtgcaggaaa ctggtcggtt
      541 acgggttcag gccacccagc agcaattaaa tttaaaagaa ggcgaaaacg aaattagttt
      601 tactgaagga aaattgactt cagccagctt ccggttcaag tactcaccca aatagccatc
      661 aaagtgagtc acgagtcatc aggcaaagat ttctttgatc aagatgtcgt cagacagagt
      721 gagtattcaa accagaaggt ggaagaaaca caaatctgct gcctgagatt tgatttcttc
      781 ccacaaacac aggctgaacc ttcttcaatt tcaattccaa atggcaatga tctatgaaac
      841 aaattttgat tccttttctt gtggcgtgct ttttggcacc tggctcaatg tgggtttcgt
      901 cggtttcacc ggtttcccaa atctcggcag tccacatacc tcaacgacag gttccagggg
      961 ggctgccttt tgtggctgac ctggtgaaaa cacaggcccg tgttccagcc tttcgccaca
     1021 tcggcccact cacggtggtc ctggcttcac gtgaccccaa aggacttcag gacttgctgg
     1081 cggcccagca aaacccggcc tcgccagagt accatcaatg gttgacacca caggaattcg
     1141 gtaatcggtt tggccgtctg ccacaggaat atgcccagct tcaggaatgg ctccgctcga
     1201 caggactcac cgtgtcccag caatggccca atcgcctcca attgacggtt gaaggcccag
     1261 cagaagctgt cgaacgggcg tttgacatcc agctcgacga atatcaactc aacgccgaag
     1321 tcgggtttgc cgccaatcga atgccatccg tcccagcgat ctttccattt atcaaaggga
     1381 tcagcgggct gcacagtttt acccggtttc aaccaatggt ctctgaaacc agcctcacac
     1441 ctggtgtcgc tcacactgga aatacttttt cgcaccgtgg cccatcaaat ttaatggctc
     1501 cggctgatat tgccacggtt tacaacttaa agcccctgct tgaccagggt ctggacggca
     1561 gcggcgtcac gattgccgtt gcggcccgat gcgactttaa cctcagcgac ccggttgcct
     1621 ttcgctcgca attcaacttg ccgccaaagg acccaatcaa agtgtttccg tttggccccc
     1681 gccctaatcg tggtggagtc gaggaagtag aagccgttct tgatgtcgaa tgggccggtg
     1741 ccaccgcccc agatgccacc gtcaaaagcg tgattgcccc ggatattgat ttctcaatcc
     1801 aggccattac caacaatatg ccggaagcca aagtcatcag tgtcagtttt ggcggctgcg
     1861 agcgatttaa cggccttgaa gtcgctgaat ttattgaaat gctttttgcc caggccgcga
     1921 cacaaggtca gtcaattctg gtctcatctg gcgataatgg cgtggctgat tgtctgcgga
     1981 ttgatggaac caccgcgccg tcagtcaatg tcctggcctc tccaccgcaa gccaccggag
     2041 tcggaggcac caatgtggat ccccagtttg ataccaatgg aaatgcaacc cggaacggtg
     2101 gtgaaaccgg atggacgggc agcggtggcg gtgacagtat ctttttcccc aaaccggcct
     2161 tccaaactgg cgttggcgtg cctgacacga cggttcgcac cgttccagac gtcgcattgc
     2221 tcgctgggag cccggcttat gcctatatca ttcgtggagc actggccggt gtgggaggaa
     2281 ccagtgtttc aaccccttgc tgggccgggt tgtgcgccat tctcaacgaa gccaggaaga
     2341 ccaacggcct tggcaatatc aatcctgaaa tctatcggtt gggaaatgcc caggcgacgg
     2401 gtgctggcgc acaggtcttt tttgatacct tgactggcaa taatagcttt ggtgcggttc
     2461 aaggatttac ctgtcaggcc gggtatgatc gggtaaccgg gtggggctcg tacaatgcgg
     2521 ctctgttggt tgagaatttt cgggctcaac ccgtaaccga taccgtggcg ccaaccattt
     2581 cactccagtc tccggttggt ggcgaagaaa tcct
//
LOCUS       DAOQDO010000749         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33850_length_2613_cov_3.288116, whole genome shotgun sequence.
ACCESSION   DAOQDO010000749 DAOQDO010000000
VERSION     DAOQDO010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33850_length_2613_cov_3.288116"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PKZ53_12315"
     CDS             complement(<1..1128)
                     /locus_tag="PKZ53_12315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41269.1"
                     /translation="MKQCPVCQKQYPDDIAFCSADGTALKLVSPDAAMTVAGVGPALV
                     PVSMTSALEGLVGQVIDGKVQVESVLGRGGMGAVYRARHLLLDRVVAVKVLRADLLGT
                     QNLGDRFLREARTAARIEHPNIITVYDFGILGDGNAYLVMEYVQGQSLRDLLKTQGAL
                     PLSTTFQLIQQICAGVDAAHTKNIIHRDLKPENIMLKDNEQGDVLVKILDFGLAKLIE
                     SSDSIANLTGTGEILGTPAYMSPEHCDGGELDHRADIYSLGVIFYEMLTGQPPFRGRV
                     VSVLSAHLTKKPEPPVQVNPHIPVDISDAIMHALEKEKDARPASVRKFFEQIEQAFAR
                     TRTHATRAIESDDTPVAARPASSSLAPPESFLTVQGNGAAAL"
     gene            1627..>2613
                     /locus_tag="PKZ53_12320"
     CDS             1627..>2613
                     /locus_tag="PKZ53_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNJ41270.1"
                     /translation="MASHVDPDSRSKMVDHSHFKPFVSSATILPEFTLRTVILGSILG
                     VIFGASSVYLALKVGLTVSASIPIAVLSITIFRAFGRASILENNMVQTIGSAGESVAA
                     GVVFTIPALLIMGYSLEISRVALIALTGGWLGVLMMIPLRRALIVKEHGKLTYPEGTA
                     CAEVLIVGEEGGSQAKTVFSGFGLGIIYKVLMSGLHLWKEVPEKVIRSFPGASISAEV
                     SPELMGVGYIIGPKVASVMLAGAVLAYLVLMPAIKFFGSLVATPLFPGTIPIAEMTSD
                     QLRDAYILYVGAGAVATGGIISLLRSMPMILSAFRSSLGGLKAGLTGGVTEAR"
BASE COUNT          562 a          663 c          726 g          662 t
ORIGIN      
        1 gagggcggct gccccattgc cctgaaccgt caaaaaagat tccggtggcg ccagcgagct
       61 tgaggccggg cgggctgcta ccggggtgtc atcactttcg atagcccgag tcgcatgcgt
      121 tcgggtccgt gcaaaggcct gttcgatttg ctcaaagaac tttcgaactg aggctggtcg
      181 ggcatctttt tctttttcga gcgcatgcat aatcgcatcg ctgatatcaa ctggaatgtg
      241 tgggttgacc tgaactggtg gctctggttt tttggtcagg tgggcactta aaacgctcac
      301 cacccggcct cgaaagggtg gttgtccggt cagcatttca tagaaaatca cgcccaggct
      361 atagatatcg gcccggtggt caagttcgcc accatcgcaa tgttccggcg acatataggc
      421 cggggtgccc agaatttccc cggttccagt cagattggca attgaatctg aagattcaat
      481 gagttttgcc agtccgaaat cgaggatttt caccaggaca tcgccttgct cattatcttt
      541 gagcatgatg ttttccggct tcaggtcgcg gtggataatg tttttggtat gggcggcgtc
      601 aacacccgcg caaatctgct gaatgagttg aaatgtggtt gaaagcggga gtgccccttg
      661 agtctttaat aagtcacgca atgactggcc ctgaacatat tccatgacca gataggcgtt
      721 gccatcaccc agaattccaa aatcatagac cgtaatgatg tttgggtgtt cgatccgggc
      781 agccgtgcgg gcttcgcgca ggaaccgatc accgagattc tgtgtcccca gcaaatcagc
      841 gcgcagtact ttcacggcca caacacgatc cagcaagagg tgacgcgccc gataaacagc
      901 ccccatgccg ccacgcccaa ggaccgattc aacctgaacc ttgccatcaa taacctggcc
      961 caccaatcct tccagtgccg aggtcattga gactggtacc agggccggac ccacaccagc
     1021 cacagtcatg gccgcatctg gtgagacgag cttgagtgct gtcccatcag cggagcagaa
     1081 ggcaatatca tcagggtact gtttctgaca gacagggcac tgtttcatga ttgatgctaa
     1141 ccgatttgaa ttggataaag ttggggaaat tgtaagtgcc agaatatcac caaagcggga
     1201 ttttgaacac tttgaactcg gagaccagac tttctttcgg cgttgcttgc cagaatacat
     1261 cctgtcggct aaaacaacac atcctaaaaa tcttggggct gaaaaaacca gggctcaggg
     1321 ctgagggctg aagacatcgg gctgaagaca tcgggctggg ggctgaagat tatcgggctg
     1381 aagacaccgg gctgaagaca tcgaactcag ggctgaggga aatacagttc tttcaagtcg
     1441 tttagccttc ccgtaatatg aggacttcat tccaaaatgg tattaaaccc gttttcttcc
     1501 gccccgagct tgcgagtctt tcagccccaa gccctgagcc ctgggttttt ccgccccgag
     1561 cttgcgagtc tttcagcccc aagccctgag ccctggttga agcaaatctt actccagaga
     1621 ggttgcatgg catctcacgt tgaccccgat tcacgttcga aaatggttga tcattcccat
     1681 ttcaaaccat ttgtcagtag tgcgacaatt ttgccagaat ttaccctgcg aaccgttatt
     1741 ctgggttcga tcctgggtgt tatttttggc gcctcgtccg tgtatctggc gctgaaggtt
     1801 gggctgaccg tcagtgcttc aattccgatt gcagtgctct cgatcactat ctttcgtgct
     1861 tttgggcggg catcaattct ggaaaacaat atggtccaaa ccattggctc ggctggcgag
     1921 tcggtggccg ccggggtggt ttttaccatt ccggcgttgt tgattatggg ctattcgctt
     1981 gaaatctcac gcgtcgcgct cattgcgttg accggcggct ggctcggcgt gctcatgatg
     2041 attccgctcc gccgcgcctt gattgtcaaa gagcacggga aactgaccta cccggaaggc
     2101 accgcctgtg ccgaggtgct cattgtaggc gaggaaggcg gaagtcaggc gaaaacggtt
     2161 ttttcagggt ttgggctggg aattatctat aaggtattga tgtcgggcct tcatttgtgg
     2221 aaagaagttc ctgagaaagt catccgcagt tttcctgggg cttcgattag tgccgaagtc
     2281 tctccggaat taatgggggt ggggtacatc atcggcccta aagttgcgtc ggtgatgctg
     2341 gccggcgctg tgctggccta tctggttttg atgccagcaa ttaagttttt tggcagcctg
     2401 gtggcaacgc cgctctttcc cgggacgatt ccgattgctg aaatgacctc agaccagctt
     2461 cgggatgcgt atattttgta cgttggcgct ggcgccgtgg caaccggcgg cattatcagt
     2521 ttactgcgct cgatgccgat gattttgtcg gctttccgtt ccagtttggg cggattaaaa
     2581 gccgggctga cgggtggcgt gacggaagcc cgg
//
LOCUS       DAOQDO010000750         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33853_length_2613_cov_3.048866, whole genome shotgun sequence.
ACCESSION   DAOQDO010000750 DAOQDO010000000
VERSION     DAOQDO010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33853_length_2613_cov_3.048866"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKZ53_12325"
     CDS             <1..708
                     /locus_tag="PKZ53_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNJ41271.1"
                     /translation="YWDTEPYLIQLGFNYTYHKRLYDVLLSESPAQAVNHHLRHTSDA
                     FLQHAVHFIENHDEDRAAARFSPQAGQRAAALIGLLPGMLLIHQGQMEGMTEKLPVQR
                     LTPLYPHTPDKELQAYYRTLLSVAAHPLFRNGRWAWTDSNWKDGVCFWRQDGETLALV
                     VVPIGRKSIRLPQYLKVDLPPREWGTSVPSTARSWWKNSKPFPVGIESDTLTIKRADV
                     RKSLHPTLEAIVIEAVS"
     gene            740..1636
                     /locus_tag="PKZ53_12330"
     CDS             740..1636
                     /locus_tag="PKZ53_12330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ41272.1"
                     /translation="MKVPVTRYERFQKVLAVLRCPGCQKIGFEFEFDAEKPEAKAQLT
                     GIIRCLHCVKTFVSEEGIVDLLPDGPTGLTVAQWSGQTGAVSWLYERIWRKRALGLMT
                     GQAFPPEREYQVLAEYLQSSLAATSHLFLDLACATGYYGRALVDFLQKNHRGDGADSV
                     IGLDISRPMLQQALEYARESQTLDHTIFIRADATSLPLADGCLAGIACGGSLNEYRDA
                     FRVLVEGKRVLERQKGRYFVMNLLKPETAGEKILQSTLTFGSGLTFFTKAEVKDLFYR
                     AGLELGRHETVGLVAFSELNRK"
     gene            1673..1936
                     /locus_tag="PKZ53_12335"
     CDS             1673..1936
                     /locus_tag="PKZ53_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41273.1"
                     /translation="MAFRLASTMILERKKWHQFCLEHQALIDMTGLPMTVFETWERFE
                     DFLLHGYLDHHPDPAAATIDALTETQKEVLTLLKQQFEDTWYT"
     gene            2343..>2613
                     /locus_tag="PKZ53_12340"
     CDS             2343..>2613
                     /locus_tag="PKZ53_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ41274.1"
                     /translation="MLNQLSEKPFFFRCVLAIGVIVAGYYLFALVMIGRYGTLTKDFG
                     WKAVPQAAGVIVTEVDAMGPAAGKLQVKDRILTINGESRIQGGARD"
BASE COUNT          626 a          637 c          705 g          645 t
ORIGIN      
        1 tattgggata ctgaacccta tctcatccag ctcgggttta actacacgta tcacaagcgg
       61 ttatatgatg tgctgctgag cgagtcacct gcccaggctg ttaatcatca cttacggcat
      121 accagtgacg cttttttgca gcatgccgtc cattttattg aaaaccacga tgaagatcgg
      181 gcggcggccc gcttttcacc tcaggctggt caacgggccg ctgccctcat cgggttgtta
      241 ccggggatgc ttttgattca tcaggggcaa atggaaggaa tgacggaaaa acttccggtg
      301 caacggctca cgccgcttta cccacatacc ccggataaag aactccaggc gtattaccgc
      361 actttattaa gtgttgcggc ccatccgtta tttcgcaacg ggcgctgggc ctggactgac
      421 tcaaactgga aggatggggt gtgtttctgg cgtcaggatg gtgaaacact ggctctggtg
      481 gtggttccaa tcgggcgaaa atcaatccgg cttccacaat atttaaaggt tgatctgcca
      541 ccgcgtgagt ggggaacatc tgtgccttcc actgctcgaa gctggtggaa aaactcaaag
      601 ccatttccag ttggcattga atccgacacc ctgaccatca aacgggcgga tgtccgcaaa
      661 agcctgcacc cgacgctgga ggcaattgtg atcgaggctg tctcatgagt ccaaccaacc
      721 aatgggaaac gagggagacg tgaaagttcc tgtgacgcga tatgagcgat tccaaaaagt
      781 ccttgcggtg cttcgctgcc cgggttgcca gaaaatcgga tttgagtttg agtttgatgc
      841 cgaaaagcca gaggccaaag cccagttgac ggggattatc cgctgcctgc actgtgtcaa
      901 aacttttgtc agtgaagaag gaattgtgga tttgctccca gatggaccaa cggggttgac
      961 ggtagcccag tggtcaggcc agacgggcgc cgtttcctgg ttgtatgagc gcatctggcg
     1021 caagcgagcc ctggggttga tgaccggcca ggcatttcct ccagaacggg aatatcaggt
     1081 actggccgaa tatttacagt catcactggc agccacttca cacctgtttt tggatcttgc
     1141 ctgtgccaca ggctactatg ggcgtgccct ggttgatttt ttacagaaaa accatcgggg
     1201 agatggggct gatagtgtga ttggacttga tatttcacgc ccaatgcttc aacaggcgct
     1261 ggaatatgcc cgagaaagcc agacattgga tcacacaatt tttatccggg cggatgccac
     1321 cagccttcca ttggctgacg ggtgtctggc tggaattgcc tgtggtgggt cgctcaatga
     1381 gtaccgcgat gcctttcggg tgctggtgga agggaagcgg gtcctggagc gccaaaaagg
     1441 tcggtacttt gtgatgaatt tgttgaagcc ggaaacggcg ggggaaaaga tccttcagtc
     1501 aactttgacg tttggaagcg gcctgacatt tttcactaaa gctgaagtga aagacctgtt
     1561 ttatcgggcc ggacttgaac tgggccgcca cgaaacagtg ggactggtgg ctttttctga
     1621 attaaatcga aagtaaccag agagtctttc aacctgttga ttttcaagag acatggcctt
     1681 ccgacttgcc tcaaccatga tcctcgaacg aaaaaaatgg caccagtttt gtctggagca
     1741 ccaggcgctg attgatatga ctggtttgcc gatgacggtg tttgagacct gggagcgatt
     1801 tgaagatttt cttctccatg gctatctgga ccatcatcca gatccggcag ccgccaccat
     1861 tgatgctctg acagaaactc agaaagaggt tctcaccctc ttgaaacaac agtttgaaga
     1921 cacctggtac acgtgaaacg gcccagaaaa ccctatgccc aggcttcacc cggtgtcagg
     1981 tgcaggaaat tccaggaatc cttttccttt ttcctctgcc ccagtatcat ttttccggaa
     2041 taaaccgatc aaaggtctaa accattggtt tgctcccacc cgtaagaacc gataaccttc
     2101 aaaaacccaa ccgaacgctt caaaaatatg tgctggtcaa accatcaggc agtctcgaac
     2161 gtgcgtgatg gctctatcag gacagtctct cacacgtgta ctgggtacca ctgtatcttc
     2221 tggaaaattc ggtgctggaa gcgcgctacg ccagagattt caggtttttt ttggagccgg
     2281 tgattgattg aaaagaacaa cattcccgtt gcccggtggt tcccaggtca tttaagaaca
     2341 caatgttgaa tcaactttca gaaaagccct ttttttttcg atgcgtcctg gcaattgggg
     2401 tcattgtggc cgggtattat ctgtttgcac tcgtcatgat tgggcgatat gggacactga
     2461 caaaagattt tggctggaag gcagtgccgc aagcagcggg tgtgattgtc actgaggtcg
     2521 atgcgatggg gcccgcggct ggaaagctcc aggtcaaaga ccggattctg accatcaacg
     2581 gtgaatcccg gattcaaggt ggggcccgtg acc
//
LOCUS       DAOQDO010000751         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33875_length_2612_cov_4.027376, whole genome shotgun sequence.
ACCESSION   DAOQDO010000751 DAOQDO010000000
VERSION     DAOQDO010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33875_length_2612_cov_4.027376"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PKZ53_12345"
     CDS             complement(<1..911)
                     /locus_tag="PKZ53_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNJ41275.1"
                     /translation="MTIAQRIFWWLALIALVTGIGWSLPETHPSLAHTSGQESVSDLP
                     PVEAFTLKMSPKHRFLAKKRPTVRGMKTVAMTPPEKVRLFTDESTLMLQIPIQQPTDS
                     DIFRASLLSAHGKTLQTFDCLPLVVNQGEPTLEVSLETAMLIDGTYRLQVFQVLSNGE
                     VVPTTDIAFSVEQGFQKLLGTPNSTGYWHTSGNKILDAANQQVRICGVNWFGLETNTY
                     IPHGLWTRGYQEMMNQMKALGYNTIRLPFSNEVLDAGRMPSNYIDPSKNPDLIGLSSL
                     QIMDQIVTYAGQIGMRVILDRHRPTAAA"
     gene            complement(1107..2201)
                     /locus_tag="PKZ53_12350"
     CDS             complement(1107..2201)
                     /locus_tag="PKZ53_12350"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNJ41276.1"
                     /translation="MYRRETITRMQVNVQGIIPVFHKTQHRNLTMMVIGIVMAKSVQL
                     TKIAPEVAIDHISHEAYVQRFERLVACEKFEVMKVMKAIASRILKYVARWQEPIVIVM
                     DRSMINDTLNLLHVAVAFGKRALPLGWVEVPHEGSSDLKLQKELLGWVKECLPRGVEV
                     VVVADREFHSIHLAKWIETEMGAEYVLRIKAKTWVEVDGQWQRAGSLAVKGESHWYQN
                     VLVTKQKGLTYRVNFVAYWDQTEDEPWLLITACGCAEQTRRWYEQRFWIEEMFSDQKS
                     RALNLESSRMTDSNRLQRLLVAVCLAYLWIMELGAIVVRGGYLYLVNSRGSKRTISLC
                     LVGLKWVHRLATQNIQPQLLSCCFDPVEES"
     gene            complement(2221..>2612)
                     /locus_tag="PKZ53_12355"
     CDS             complement(2221..>2612)
                     /locus_tag="PKZ53_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41277.1"
                     /translation="IYNGSQNWIRGFIFGSDESLSANSWGGPQVCHFKSDTTQLSSNN
                     KITNNWFGLSSTGDSIESNLTTPGISLEQGASFNLIEQNTICAYGLPLILSGPGEINS
                     VKGNLIDWGLLFLRGGMVHFLRSKIIR"
BASE COUNT          676 a          718 c          556 g          662 t
ORIGIN      
        1 gctgctgccg ttgggcggtg gcgatccaga atgacccgca taccgatttg cccggcatac
       61 gtcacaatct ggtccataat ttgcaggctg gaaagcccaa tcaaatcagg gttttttgac
      121 ggatcaatgt aattgctcgg catccgtcca gcgtccagca cttcgttgct gaatggaagc
      181 cgaatggtat tgtaccccag ggctttcatc tggttcatca tttcctgata cccacgcgtc
      241 cagaggccat gagggatata ggtatttgtt tcaagcccaa accagtttac accacaaatc
      301 cggacttgct ggttggcggc atccaggatt ttgttgccgc tggtgtgcca gtacccggtg
      361 ctattggggg tacccagcag tttttggaat ccctgctcaa ccgaaaacgc aatgtccgtt
      421 gtcggcacaa cctccccatt ggaaagaact tgaaacactt gcagccgata ggttccgtca
      481 atgagcattg cggtttcaag actgacttca agcgtcggtt ctccctgatt gaccaccagc
      541 ggaaggcagt caaaagtttg cagtgtcttc ccgtgggcag aaagcaagct ggctcgaaaa
      601 atgtcgctat ccgtaggctg ttgaatcggg atttgcagca tcagggtgga ttcatcggtg
      661 aaaagccgga ctttctccgg cggggtcatg gcaaccgttt tcattccgcg aaccgtgggg
      721 cgttttttgg ccagaaaccg atgtttcggc gacattttca gagtaaaggc ttccaccggg
      781 gggagatcgg aaaccgattc ctgccctgat gtatgagcta atgatggatg ggtttcaggc
      841 aaactccatc caatcccagt cacaagggca atgagtgcca gccaccagaa tatccgttga
      901 gctatagtca taatttcgac tccacgatga agaatcatca aattgaaaat tgaaaggtgc
      961 tgccttactt tcaaaaggcg caaaccagca aaaaaaagct cattgattta gaaaatggag
     1021 ggtgaacgtt ccacaaccaa taaacccaga tgaatccagg ctggaggaac tgaatggtgt
     1081 ctggtcttga ctggggatta ttattttcag gattcctcga ccgggtcgaa gcaacagcta
     1141 agtaattgag gttgaatatt ttgggtggct aaacgatgaa cccacttgag accgaccaaa
     1201 cacagactga ttgtgcgctt tgagccgcga ctgttgacca gatacaaata ccctccccgg
     1261 accacaatcg cacctaattc cataatccat aaataggcca gacaaacggc caccagtaag
     1321 cgctgaagtc ggtttgaatc agtcatccgg gaggattcca gattcaaggc ccgacttttc
     1381 tggtctgaaa acatctcttc aatccaaaat cgctgttcgt accagcgccg ggtctgctcg
     1441 gcacagccgc aggcggtgat caacaaccac ggctcgtctt cggtttggtc ccagtacgcc
     1501 acaaaattca cccgatacgt caaccctttc tgtttggtca ccaggacgtt ctgataccaa
     1561 tgcgattcac ctttgaccgc cagacttccc gcccgctgcc attgcccatc cacttccacc
     1621 cacgttttcg ctttgatgcg cagcacatac tccgccccca tctccgtctc aatccatttt
     1681 gccaaatgaa tcgaatgaaa ttcccgatcc gccaccacca ccacctcgac cccgcgcggg
     1741 agacattcct tcacccaccc caacaattcc ttctgcaact tcaggtccga actcccctcg
     1801 tgcggcactt ccacccaccc caatggcaat gcccgtttcc caaacgccac cgccacgtgc
     1861 aacaaattca acgtgtcatt gatcattgat cggtccatta cgatcacgat tggttcctgc
     1921 caccgcgcca catacttcaa gatccggctc gcaatcgctt tcatcacttt catcacctca
     1981 aatttctcac acgccaccaa ccgttcaaat cgctgcacgt aggcttcatg gctgatgtgg
     2041 tcaatcgcca cttccggcgc gattttcgtc aactggactg atttcgccat cacgatcccg
     2101 atcaccatca ttgtcaaatt ccgatgctgc gttttatgaa acacgggaat gattccttgc
     2161 acattgacct gcattcgggt gatagtttct cgacggtaca ttttttgcct ccatttgttt
     2221 ctatctaatg atcttagatc gcaagaaatg taccataccc cctctcaaaa ataataatcc
     2281 ccagtcaatt aaattcccct taacagaatt aatttcacca ggaccagaca ggattaatgg
     2341 aagcccatat gcacaaatag tattttgttc tattaaatta aaacttgccc cctgttccaa
     2401 agatattcct ggcgtagtca aattgctttc aattgaatct ccagttgagg acaagccaaa
     2461 ccagttattc gtaattttat tatttgacga taattgggtt gtgtcgcttt taaaatgaca
     2521 aacctgaggc cctccccatg aatttgcgga gagagactca tcagagccaa aaataaaacc
     2581 acgaatccaa ttttgacttc cattatagat ac
//
LOCUS       DAOQDO010000752         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33877_length_2612_cov_3.823621, whole genome shotgun sequence.
ACCESSION   DAOQDO010000752 DAOQDO010000000
VERSION     DAOQDO010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33877_length_2612_cov_3.823621"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKZ53_12360"
     CDS             complement(<1..187)
                     /locus_tag="PKZ53_12360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100113.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNJ41278.1"
                     /translation="MEPIKADEISQIIRQQIENFQVGVTVSEVGTIIKVGDGIAEIHG
                     LERAMAGELLDLPNNVKG"
     gene            complement(464..1006)
                     /gene="atpH"
                     /locus_tag="PKZ53_12365"
     CDS             complement(464..1006)
                     /gene="atpH"
                     /locus_tag="PKZ53_12365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNJ41279.1"
                     /translation="MSITALANRYARALVDVTAAKGEMKEVFTEISAFQTLVVTHAEL
                     HEVVVNPTIPQNQKEVLLVTLTTRMKLRPTTVNFLSLLLRNQRLAYLGEIVEALRREI
                     DIRTNVVAADVTTAGALAETQKVTIEKRLQQVTGKAVRVNYTVDPTIIGGVVSRIGSR
                     IYDGSIRNQLDSYRSVLSQN"
     gene            complement(1207..1731)
                     /locus_tag="PKZ53_12370"
     CDS             complement(1207..1731)
                     /locus_tag="PKZ53_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNJ41280.1"
                     /translation="MMFPLLTSLLASVELLSSMPLGYRFINFAIFAIALGAVLYKPLT
                     QGLQARANSIEEELQRAGKEIAAAEAKMKELDTRISRLDDEISDIKKQSERDAKAERE
                     RIAQTTKEDAERLRAMARLEIEGAMKAARTELRAFAASQAVEMAEAIIRRELKDTDRD
                     QLVNRYVDRMERVN"
     gene            complement(1728..2210)
                     /locus_tag="PKZ53_12375"
     CDS             complement(1728..2210)
                     /locus_tag="PKZ53_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNJ41281.1"
                     /translation="MIDACLLPQLLLFAELLSVDYSLVVIIVLFIVLAILLNSLIFNP
                     ILKVLEERERLTSGSISGARHLLEEYDRRLKDYESALKDARLAAYEQMEKHRSAAMKE
                     RTDMLNTVRTETTAEIAKAKNEIEAQVKKARTTLEKDASVMAATISTSILGRQVGGPR
                     "
BASE COUNT          636 a          619 c          678 g          679 t
ORIGIN      
        1 tgcctttcac gttgttgggc aaatcgagca attcgccggc catggcgcgt tccaggccgt
       61 ggatttcagc gattccgtcg ccgactttga tgatcgtgcc aacctcagaa accgttactc
      121 ccacctggaa gttttcaatc tgttggcgaa ttatctggct aatttcgtcc gctttaattg
      181 gttccatagt ctcttcctgt cagggaaatc ccgcaaataa ttcaattcaa aaaactaacc
      241 gtgttttgcc aggcattggc gatgagtgga gttcaccaaa agagtgtctg gagatgaggg
      301 taaggggaat cccctgaaaa aaaatgaatg cgtggaaaag aaaagctctc tatccaaaca
      361 caacctctgt gtgaaacccg actcgaatga agaattgaga atgaagaatg cagaatgaag
      421 aaaattttgg tttttcattc ttcattcttc attcttcatt ctcctagttt tggctcaaaa
      481 ctgagcgata gctgtccaac tgattgcgaa tggagccgtc gtagatgcgg cttccaatcc
      541 gtgagaccac gccaccaata atcgtcggat ccaccgtgta gttgacccga acggcttttc
      601 cggtgacttg ctggaggcgc ttttcgattg tgactttttg ggtttcagcc agggcgccag
      661 ccgtcgtgac atcggcggcg accacattgg tccgaatgtc aatttcgcgc cgtaatgcct
      721 ccacaatttc gcccagatag gccagacgtt gattgcgcaa cagcagactc aggaaattca
      781 ccgtcgtcgg gcgcagcttc atgcgggtgg tcagtgtgac cagcagcact tctttctggt
      841 tttggggaat ggttggattg accaccactt cgtggagttc ggcgtgggtt acgaccaggg
      901 tctgaaacgc gctgatctca gtaaatactt ctttcatttc gcctttggcc gcagtcacat
      961 caaccaaagc acgggcataa cgattggcaa gggcggtaat actcataaat aactcagttt
     1021 gtggaaatga agaccttttt cttgggctga agacttgcaa tcttcggggc tgaagacacc
     1081 gggcttgggg ctgaaaaaac cagggcttag ggctgaaaac tcgcaagctc agggctgaag
     1141 aaaacgggta aaaccaatct caagcccgat attttcagcc cgaagtcttc agccccgagc
     1201 ccccaatcag ttcacccgct ccatccgatc aacatagcga ttgaccagtt gatcgcggtc
     1261 ggtgtctttc aactcacggc ggataattgc ttcggccatt tcgactgcct gtgaagcggc
     1321 aaaggcgcgg agttcggtcc gggcggcttt catcgcgcct tcaatttcaa ggcgggccat
     1381 ggcgcgcaac cgttccgcat cttctttggt ggtttgggca attcgttcgc gctcggcttt
     1441 ggcatcccgc tcagattgtt tcttgatgtc gctaatttcg tcatcaagtc ggctgatccg
     1501 ggtatctagc tctttcattt tggcttcggc ggcggcaatt tcttttccag ctcgctgaag
     1561 ttcttcttcg attgaattgg cgcgagcctg caaaccctga gtgagcggtt tgtagagaac
     1621 agccccaagt gcaatcgcaa agatggcaaa gttgataaag cgatagccga gcggcattga
     1681 ggagaggagt tcaactgagg ccaacaggct ggttaataga ggaaacatca tcgtggacct
     1741 ccaacttgac gaccaagaat tgaagtcgaa atcgtggcgg ccatcactga agcatctttt
     1801 tcaagtgtcg tgcgggcttt cttgacctgg gcttcgattt cattcttggc tttggcaatc
     1861 tcagccgtag tttcggtgcg cacggtgttg agcatatcgg ttcgttcctt cattgcggct
     1921 gaacgatgtt tttccatttg ttcataggcc gcaagacggg catctttgag tgccgattca
     1981 tagtctttga gccggcggtc gtattcttcg agcaggtgcc gggcaccgct gatggaaccg
     2041 ctggtcaggc gttctcgctc ttcgagcact ttcaggatcg gattgaagat caggctgttg
     2101 agcagaatcg ctaaaacaat gaaaaggacg atgataacga cgagagaata atcaacgctc
     2161 aacaattcgg cgaagagcag aagttgaggt aataggcatg cgtctatcat agcgaatgag
     2221 gttcaacctt tagagacgga aaagtatgag ggatgaaact ggtcctaaaa agaagagcgt
     2281 tgatagcata gcggggtagg agtgtcaagg acagagtcct tttcagtgtg gataagtaaa
     2341 agcatttgca gcacttggct tgaggactgg ctcaactttc agtcaacctg ggtcgaaacc
     2401 ccagagccgg cgattcccga aatgccctgt ttgtgggaga ctcgatattt taagtgctca
     2461 ggcaagtcta agaaccagtg aaagtggttg gactttcgca gtccagttca atcaaaattt
     2521 gggcagcttt ggaaaaatct tcgttctctc aagatcgctt caaatccgcc tctctccaaa
     2581 tcattcatat ctgtatttaa ggagaaaacc ct
//
LOCUS       DAOQDO010000753         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33924_length_2610_cov_3.523288, whole genome shotgun sequence.
ACCESSION   DAOQDO010000753 DAOQDO010000000
VERSION     DAOQDO010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33924_length_2610_cov_3.523288"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..216
                     /locus_tag="PKZ53_12380"
                     /pseudo
     CDS             1..216
                     /locus_tag="PKZ53_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            359..505
                     /locus_tag="PKZ53_12385"
     CDS             359..505
                     /locus_tag="PKZ53_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41282.1"
                     /translation="MRSGVGSNGFTRFGKPGWHGSERMLVFSTRCGSGTSALAPRPQY
                     VVAR"
     gene            541..2394
                     /locus_tag="PKZ53_12390"
     CDS             541..2394
                     /locus_tag="PKZ53_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ41283.1"
                     /translation="MALDRDLSSIQQARDLVEQAHRAQAILATYSQEQIDTIVQKMAE
                     AGQAHSTRLAEMAVQETGFGIASDKDIKNRFAAKDVYEFFKSLKTVGVVRETDTLIEV
                     ADPRGVVCAIIPSTNPTSTAIFKAIISIKSRNSVVLSPHPSAANCIGETARIVREAAE
                     AAGMPQGAIGCLTVSTQGGTEALMKHEKTAVILATGGIGLVRAAYSSGKPAFGVGPGN
                     VPAMIERSADVPKAVADILAGKTFDNGTVCASEQAVVVEKVLDAEVRAQFAAQGGYFL
                     NRDQAEKLAKVVVLPSRGLNPGIVGKSVQKIAEMAGISVPKETRALMVELTGVGRDFP
                     LSLEKLSPILAYYVVPDFETGATVCSQILRFGGMGHSIGIHSQNRNKIREFGMRQPAS
                     RILVNTPTTHGAIGFSTELAPSMTLGCGTWGGNVTSDNISPLHLMDIKRIAFETFAVT
                     RPTSRPSSPPAVKASVIPAPSPGILAGGNSPSAIVHQVAPGSAPKAAPPGSATKLPSS
                     LDRASIAALVDQFLAERKPALNQALKAPASAPVAAPAPAAPPPPAPAPAPPAKVEKAE
                     SPKAAEFVCEDDVRRALVENRKIVTDKKTIITPAARDLGREYSIFAGYAES"
BASE COUNT          580 a          697 c          729 g          604 t
ORIGIN      
        1 attggcgcag tcgaagcatc tgacgccatg gtcaaagccg ccaatgtgat cctggttggc
       61 aaagaatatg ttggtgccgg gtacgtgaca gtttttgtcc ggggcgatgt tggtgctgtc
      121 aaagcggcca cagatgctgg cgccgctgcc gcacgacggg ttggtgaact cattagtgtt
      181 cacgtcattc cgcgaccaca cgccgaagtt gagcgtgcct ttgaggatat gggttggggc
      241 ctgaccaaac acctcgaagg gaataatccc aaagaactga cggatggtgg tggaggtaag
      301 cgagcactgc cttcatcaaa agaagacacc aaaaaatcat agcgaacctg tgatgtagat
      361 gcgctctggt gtggggtcaa acgggtttac caggtttggg aagcccggat ggcacggctc
      421 agagcgcatg ctggtttttt cgaccagatg tggttcgggc acctccgcac tggcaccgcg
      481 cccgcagtat gtggttgcca ggtagtgatt gaacccgcca tctggtgagg agattgattc
      541 atggctttgg atcgcgattt atcttccatt caacaagcgc gtgatttggt ggagcaagcg
      601 catcgcgccc aggcaattct ggcaacctat tcgcaggaac aaatcgacac gattgttcaa
      661 aagatggctg aggctggcca ggctcattct acgcgtctgg cagagatggc tgtccaggaa
      721 accgggtttg ggattgcctc ggacaaagac atcaaaaatc ggtttgcggc caaagatgtc
      781 tatgaatttt tcaaatcgct caagacggtt ggtgtggtgc gtgagactga cacgctgatt
      841 gaagtcgcgg atccacgtgg cgtcgtgtgc gccatcattc cttcgaccaa ccccacgtca
      901 actgccatct ttaaagccat tatttcaatt aaatcacgca attcggttgt gttgagccct
      961 catccatcgg cggcgaattg catcggcgaa acggcgcgaa ttgtccgcga agccgccgaa
     1021 gccgccggca tgccgcaagg tgccattgga tgtctgacgg tttcaaccca gggtgggacc
     1081 gaagccctga tgaaacacga aaaaaccgct gtcattctgg cgactggcgg gattgggttg
     1141 gtccgggctg cctatagttc tggaaaacca gcctttggtg ttggtccggg aaatgttcca
     1201 gccatgattg agcgtagtgc cgatgttccc aaagcggtgg ccgatattct ggccgggaaa
     1261 acctttgaca acggtacggt gtgcgcttca gagcaggcag tggtggttga aaaggtgctt
     1321 gatgcggaag ttcgagccca gtttgcggct caaggcggtt attttctgaa ccgtgatcag
     1381 gctgaaaaac tggccaaagt ggtggttttg ccaagtcgag gattaaaccc agggattgtc
     1441 ggtaagtcag tccagaagat cgctgaaatg gctggcatta gtgtccccaa agaaaccagg
     1501 gccctcatgg ttgaattgac cggtgttgga cgcgactttc cactctcgct cgaaaaactg
     1561 tctccgattc tggcttatta tgttgtgccc gattttgaaa ccggcgccac ggtctgttca
     1621 cagattctgc ggtttggcgg gatgggccat tcaattggta ttcactccca aaaccgcaac
     1681 aagatccgtg aatttgggat gcgccagcca gcttcgcgaa tcctggtcaa tactccgacc
     1741 actcacggcg ccatcggatt ttcaactgaa ctggccccca gcatgacgct cggatgcggt
     1801 acctggggcg ggaacgtgac ctccgacaat atttcgccac tccatttgat ggacatcaag
     1861 cggattgcct ttgaaacctt tgccgtcaca cgtccgacgt cgagaccttc atcgccacca
     1921 gcggtcaagg cgagtgtgat tccggcacct tcaccgggaa ttcttgctgg cgggaacagt
     1981 ccatcagcga ttgtgcatca ggttgcacct ggctcggccc caaaagccgc gcctccgggc
     2041 tctgcgacca aattgccttc ttctctggat cgagcctcga ttgcggcact ggtcgatcaa
     2101 tttctggctg agcgcaaacc cgcgctgaac caggcactca aagccccagc cagcgccccg
     2161 gttgctgcac cggcgccagc cgccccgcca ccaccggcgc cagccccagc cccgccagcc
     2221 aaagttgaaa aggctgaatc tcccaaagcg gctgaatttg tgtgcgaaga tgacgttcgg
     2281 cgggcgctgg ttgaaaatcg caaaattgtg accgacaaga agacgatcat caccccggct
     2341 gcccgtgacc tcgggcgtga atacagcatc tttgccggtt atgccgagtc ctgaggcaaa
     2401 acgaattctg gttgctgcat ctatctcaat aaatctcccc acacaacttt gaggacgttt
     2461 ccaaagcttt gtcgcacgag cggcccgtgg ggagattttt gtcttcctca aaccctcgac
     2521 ccggtactgc ttccattttt tcagtttccc caaactcttc tgtaatccaa gctggatttt
     2581 tacgattagc agggtgcgcg tttttttaag
//
LOCUS       DAOQDO010000754         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_33976_length_2608_cov_2.502938, whole genome shotgun sequence.
ACCESSION   DAOQDO010000754 DAOQDO010000000
VERSION     DAOQDO010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33976_length_2608_cov_2.502938"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKZ53_12395"
     CDS             complement(<1..376)
                     /locus_tag="PKZ53_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41284.1"
                     /translation="MKHAGKLIVLGVGALVVLIVVLILLRNVNYAEEGTVKVLTQWGS
                     IRRVYAPSDGWFTTWLPGQRAYEVNLRSFTETATPRVTSKDNAALQVPISVTAFTESA
                     KVADYVRKYGFSDEERHKRRNEI"
     gene            complement(631..>2608)
                     /locus_tag="PKZ53_12400"
     CDS             complement(631..>2608)
                     /locus_tag="PKZ53_12400"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNJ41285.1"
                     /translation="TLSYRRLRARISPPTLTISQLEKKLPGLAQADWHRHFTTRMSPR
                     FFPGLEQLKATADLINRQCPLATEATLNAATALCQHQFYFFFHQLGHESDEINWLDGW
                     PRKHYTEIVYGAALTPPRDIKFTWEIGRFLHTNTLGRAYALTNDEQFATEFFSQLESF
                     DRASPCEFGPHWLCAMEVGIRAVNLVTGFYFFRHSPGCTPERVARFLRMLYEHACFVE
                     QNLENTHHITSNHYLSNLVGLLFIGVMFPEFDQSHHWREFAWREILTEFDKQVYPDGA
                     DFEASTSYHRLVAELFACSFWLGEHNELQLPDEARSKLTRMFEFTRTCLRPDGLIPQI
                     GDTDNGQLLTFAERHPIDHAYLPFLGVALGLEVVLPNLNPEYRQHIPAALPGQSSAPE
                     PEAEAIWFGGRVPEGIVEPSISTAFPDAGIYVLRQADDFLIVNCGDNGIGGFGSHGHN
                     DALAVEVWSKGRAWLVDAGTYSYTGNLEARHLFRSTEFHNTARIDGEEIKPILKSAPF
                     SLGANVKPHIRTWKTGEIEDLLEAEHDGFKRLPDKIHHQRRILFNKPDREWVVEDRFI
                     SEPSQTKHLIELFFNFDAGITIESATGSQIIAKDDQNRSFYIQTVHSSVVLNAEVVNR
                     WVSYGYGHRTNSQGVVWSVESKLPVILTWRMFAI"
BASE COUNT          635 a          682 c          685 g          606 t
ORIGIN      
        1 aaatttcatt gcggcgtttg tgacgttctt catcgctaaa cccatatttc cgaacgtagt
       61 ccgcaacttt ggccgattcg gtaaaggccg tcacactaat cgggacctgg agtgcggcgt
      121 tatcttttga agtcacccgt ggtgtagctg tttcggtaaa tgaccgcaaa ttgacttcat
      181 aggcacgctg gccgggcagc caggttgtaa accacccatc cgaaggggca tacacccggc
      241 gaatagatcc ccattgggtg agcactttga ccgtaccttc ttcggcataa ttgacattgc
      301 gcaacaagat taaaaccaca atcagtacca ccagagcacc tacacccagc acaatcagct
      361 ttccagcatg tttcataaac aaactccaaa gttgtgaatt tcagggttga agacttcggg
      421 ctcagggctt ggggctcagg gcttggggct cagggctgaa gaaaacggga cgtaactctt
      481 cagccccaag ccctcagtct tgagccccga gcttgcgagt cttcagcccg tggtcttcag
      541 ccccgagctt gcgagtcttc agcccttagc cccaagccct cagtcttcag cccttagccc
      601 caagccctca gtcttcagcc ccaatttcaa ttaaatcgca aacatccgcc aggtgagaat
      661 caccggcagt tttgattcca ctgaccagac gacgccttgt gaattggttc ggtgaccata
      721 tccatatgat acccagcggt ttacgacttc ggcattcaag acgactgaac tatgaaccgt
      781 ctgtatataa aaactccgat tttgatcatc tttggcgatg atttgagaac cggtggcgga
      841 ttcaatcgtg ataccggcat caaagttgaa aaacaattcg atcaaatgtt tagtttggct
      901 cggttcggaa atgaagcggt cttcaaccac ccattcccga tcaggtttat tgaacagaat
      961 ccgccgctgg tgatggattt tatcgggaag gcgtttaaac ccatcgtgtt cagcttcaag
     1021 caggtcttct atttcgccag ttttccaggt ccgaatgtgt ggtttgacgt ttgcgccaag
     1081 cgaaaatggg gcgcttttca aaatcggttt aatttcctct ccatcaatcc gggctgtgtt
     1141 gtgaaactcc gttgagcgaa aaagatgacg agcttcaagg ttgcccgtgt aactgtatgt
     1201 tcccgcatca acaagccacg cccgaccttt agaccagact tcaacggcca gcgcatcatt
     1261 atgcccgtgc gaaccaaacc cgccaatccc gttgtctcca caatttacaa tcaggaaatc
     1321 atcagcctgg cgcaggacat agatcccggc atcaggaaag gcggtcgaaa tggaaggttc
     1381 gactattcct tccggcaccc gtccgccaaa ccagatggct tcagcttctg gttccggggc
     1441 ggaggattgt ccgggcagtg ccgctggtat gtgctggcgg tactcggggt tgagattcgg
     1501 caataccacc tctaacccaa gcgccacacc caggaaggga agataagcat ggtcaatcgg
     1561 atggcgttcg gcaaaggtca aaagctgtcc gttgtcggtg tctccgattt gtggaatcag
     1621 cccatctgga cgcaggcacg ttcgggtaaa ttcaaacatt cgggtcagtt ttgaacgggc
     1681 ctcatccgga agttgaagct cattgtgttc cccaagccag aaactgcagg caaagagttc
     1741 cgccaccagc cggtgatagc tggtggaagc ctcaaaatcg gccccgtccg gataaacctg
     1801 tttgtcaaat tcggtcagga tttcacgcca cgcaaactcg cgccagtgat gtgattggtc
     1861 aaattcggga aacatcacgc caatgaaaag cagcccaacc agattagaaa ggtagtgatt
     1921 gctggtgata tgatgagtat tttcaaggtt ttgctcaaca aagcaggcat gttcatagag
     1981 catgcgcagg aagcgggcaa cccgttccgg cgtgcagcct ggtgaatgcc gaaaaaaata
     2041 aaacccggtg acaaggttca ccgcccggat gccgacttcc atggcacaca gccagtgtgg
     2101 gccaaattca cagggagacg cccggtcaaa cgattcaagc tgcgaaaaaa actctgtggc
     2161 aaactgttcg tcgttggtta acgcgtaggc ccggcccagt gtgttggtat gcagaaatcg
     2221 tccaatttcc caggtgaatt tgatgtcacg cggaggcgtc agtgccgctc catacacgat
     2281 ttcggtgtag tgttttcgcg gccagccatc gagccagttg atctcatccg actcgtgccc
     2341 aagttggtgg aagaagaaat aaaactgatg ctggcacaag gcggtggcgg cgttgagggt
     2401 tgcttcggtt gccagcgggc actgccggtt gatcaggtcg gcggtggcct tgagttgctc
     2461 caggccagga aaaaaccgtg gcgacatccg ggtggtgaaa tggcggtgcc agtcagcctg
     2521 ggccagcccc ggcaatttct tttcaagttg cgaaatggtc aatgttggcg gggaaatccg
     2581 ggcccgaagc cgtcggtatg acaatgtg
//
LOCUS       DAOQDO010000755         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34002_length_2607_cov_2.453762, whole genome shotgun sequence.
ACCESSION   DAOQDO010000755 DAOQDO010000000
VERSION     DAOQDO010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34002_length_2607_cov_2.453762"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..2516
                     /locus_tag="PKZ53_12405"
     CDS             315..2516
                     /locus_tag="PKZ53_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41286.1"
                     /translation="MKNEPSSTTQTAPAPWLYHPALDLIIGCGAWSLPLLLLDYGIGP
                     GKPIAVLVGFYALALVFNYPHYMATLYRAYRTHEDVTKYKYFTLYFTGLIVLTAILTH
                     WSYELLPWVFTTYVTWSPWHYTGQNYGLAMMFARRAGAQPPTWQRNLLYTAFHSSFFI
                     LFFTFHSGPSSDPVLVSLGFPIGFGYWARVILVGLFLVTSLWSIWLLVRQSSWKAMLS
                     PIVLLSTQFLWFVAPTLVEFGSGVSIAQTRYSSGVLAVMHSAQYLWITSYFARREAQA
                     SATSPGWSLAAYFGTLIVGGIALFLPGPWLISYVFHYDFAASFLIFTAIVNIHHFMMD
                     GVIWKLRDGRIAALLLTPSTGKAAESSQYKVALFDAVRWMTGPTSGARVFRVATAIGL
                     LFLAGLDLVKFYCTTNEQNLPNLVRAAELNPYDSVAQRRLAQAQLRQNNTDQTIIALQ
                     KAVKVNPHDPNTQHLLGKMLLERDRLTEAYAHYQQIVVYLPNDANALMNYAILEAQFG
                     DTDKAIASWEQVLQLDPSQVNAHRYLAENYKQQGNFQKAMFHAERFLESARKLPPDQV
                     TPVDVMMITLRLAQSEAQLNAQPAAITHFEAVIAQSRQLGNRDVEALALVQCGEAYGQ
                     LKQFDKALSVYQQGLQLERPNSSQADLAVDWFNYGQLLQSCQAPNRLILACFLKAETL
                     LLDGGAEVQSSLNVVRRARIEVESRPDTDAAQTRRQVEVVLQEALRFRKNG"
BASE COUNT          589 a          670 c          655 g          693 t
ORIGIN      
        1 ggcatcaatc ggttgcggtt caagcagttg ccggcaggat gccggcgttc ccaggaggga
       61 atctttttct gaaaaactat aggaatgaag aatgtggttt gtgattcgtg attcgtgatt
      121 cgtgattaga aatcaatgct ttccaagaag aacaaggtca gtatggccag cacaccttca
      181 ttgaaagtaa cttcccgtca gtccttttcg tcctttctgt cctttttgtc ctttgattcg
      241 ggctcaatca cctatcgttg aggctaaagc tgagcaggca gccagtattg tgggcgaaat
      301 ttggggaatg aaaaatgaag aatgaaccct cctcaacaac ccaaacggcg ccggcgccgt
      361 ggctgtatca tccggcgctt gacctgatta tcggctgtgg ggcctggtcg ttgcccctgc
      421 tcttgcttga ttatggaatc ggaccgggaa aaccaattgc tgttctggtt gggttttatg
      481 cgctggcgct ggtgtttaac tacccgcatt acatggccac gctgtatcgg gcctatcgca
      541 cccacgaaga cgtcaccaaa tacaaatatt tcaccctcta cttcaccggg ttgattgttt
      601 tgaccgcgat tctgacgcac tggtcatatg aactgctgcc atgggttttc acgacgtacg
      661 tgacctggag cccctggcac tacaccgggc aaaattacgg actggccatg atgtttgccc
      721 gacgggctgg ggctcagcca cccacctggc aacggaatct gttgtacaca gcctttcact
      781 cttcgttttt tattctcttt ttcaccttcc acagcggtcc atccagtgat ccggtactgg
      841 tctcccttgg ctttccgatt gggtttggat actgggcacg ggtaatactg gtggggctgt
      901 ttctggtcac aagtctctgg agcatctggc ttttggtccg acaatcgagc tggaaagcga
      961 tgctgtcacc aattgtgttg ctttcgacgc agtttctctg gtttgtggca ccaaccctgg
     1021 tggaattcgg atcaggtgtc tcaattgccc agacccggta cagttctggc gtcctggccg
     1081 tgatgcactc ggctcaatat ctatggatta ccagttattt tgcccgccgc gaagcccagg
     1141 ccagtgcaac ctctccaggc tggagcctgg ctgcctattt tggaacgctg attgttggtg
     1201 gaattgcgtt gtttctgcct ggtccgtggc tgatcagtta tgtgtttcac tatgactttg
     1261 ccgccagctt tttgatcttc acggcgattg tcaatattca tcactttatg atggatggcg
     1321 tgatctggaa attgagggat ggacgcatcg ccgctttgct cctgacacca tcaactggaa
     1381 aagcagccga gagttcacaa tataaagttg cgttatttga tgctgttcgc tggatgaccg
     1441 gcccaacttc cggagcacga gtctttcgcg tcgcaactgc catcgggttg ctctttcttg
     1501 ccgggctgga tctggtcaag ttttactgta caaccaatga acagaacctt ccaaatctgg
     1561 tccgggcggc agaactcaac ccgtatgatt cagtcgctca acgacgactg gcccaggctc
     1621 aacttcgaca aaacaatact gatcaaacca ttattgccct gcaaaaggca gtgaaggtca
     1681 accctcacga cccaaatacc cagcatttgc ttgggaaaat gctgcttgaa cgggatcggc
     1741 tcaccgaagc ctatgcccac tatcaacaaa tagtggttta tctccccaat gatgccaatg
     1801 cgttgatgaa ttatgccatt ttggaggccc aatttggcga tacagataaa gcgattgcca
     1861 gttgggaaca ggtcctgcaa cttgacccga gtcaagtgaa cgcccatcgg tatctggccg
     1921 aaaactataa acaacagggc aatttccaaa aggccatgtt tcacgctgaa cggtttctgg
     1981 aatcggccag gaaactcccg ccagatcagg tcactccagt tgatgttatg atgatcactt
     2041 tgcggctggc ccaatctgaa gcccagttga atgcccaacc ggcggccatc acccactttg
     2101 aagcggtcat cgctcagtcc cggcagcttg gaaaccgtga tgtcgaagcc ctggcactgg
     2161 ttcaatgtgg ggaagcctat ggccagctca aacaattcga taaagccttg agtgtgtatc
     2221 aacaaggact gcagcttgag cgtcccaaca gcagccaggc ggacttagcc gttgactggt
     2281 ttaactacgg gcagttgtta caaagctgtc aggcgcccaa ccgcctgatt ctggcctgtt
     2341 ttctcaaagc tgagacgctg ttgttagatg gtggagcgga agttcaatca tctttgaatg
     2401 tcgtgcgccg ggcccggatc gaagttgaat cacgacctga cactgatgcc gcccagaccc
     2461 gccgtcaggt ggaagtcgtt ttacaagagg cacttcggtt tcgaaaaaat gggtagaatg
     2521 agttgtcctc ttgaataaca tatatttagt cgattcttca ttccccaaat ttcgcccaaa
     2581 atactgactt gttccccagt tttggta
//
LOCUS       DAOQDO010000756         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34020_length_2606_cov_2.671501, whole genome shotgun sequence.
ACCESSION   DAOQDO010000756 DAOQDO010000000
VERSION     DAOQDO010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34020_length_2606_cov_2.671501"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKZ53_12410"
     CDS             <1..527
                     /locus_tag="PKZ53_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41287.1"
                     /translation="IGSKDAFPMICQRCRTLNAEVTNVCVECGLDLGKTPPAPARVPR
                     ATARSKQAISPAPGTTALPGLTCPLCHTPFRLSWTRFLTSPWSIYQCPQCLNRLKLRD
                     KGLWVGFLRILLVGIVTPPVGIGLMKYSNGDILISGIASLIVCCAICVPCDKYLNEHL
                     RELTPLQSPPPPKN"
     gene            539..1417
                     /locus_tag="PKZ53_12415"
     CDS             539..1417
                     /locus_tag="PKZ53_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNJ41288.1"
                     /translation="MRYFIPLQPHSFDPALTLLGGQTFRWRYSPEDNTYQGIVNGQVW
                     RLSVSYGGMRVHQDEPSPTGNPPFPIQVFHYFDLRRDYAQALAILHQDPIFLQATRTI
                     TGLKLLNQNWFEVIVSFVISANNHIKRISSIIDRLCTTYGTPIPGQPLACAFPTPQQL
                     AEAQLDVLREQCGTGYRDQYLVGLAQHITDHSSHWQQASQLSTAELRDLLLGLPGVGP
                     KVSDCILLFGFGRWDVFPVDTWIRRIMIDLYFPGQSPSDQVIRQFALEQFGQLAGLAQ
                     QYLFEAARTQKATPAR"
     gene            1708..>2606
                     /locus_tag="PKZ53_12420"
     CDS             1708..>2606
                     /locus_tag="PKZ53_12420"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ41289.1"
                     /translation="MQGDLTTICLPDLLRSIYVERRTGELIFSQGEQRKQIFFELGNI
                     VFSASNQAQDRIGEVLLRHGLLSPQDFDRAVEATKSGKRFGKALVEMGIMGERELITN
                     VTFQVLDIIYSLFAWTTGSYQFIEGENRVADELKLKLTTARIILEGVRRIEDFEIIRR
                     GLGDINRLVAPSSSMLLRMQTTSLKPVERKVVDLVTQPTDLLKILITTDATPELTLKS
                     IYGLLSAGILEQTQVAELSHRTGKFAVPNEIAAQPTPPPPPTVTRTTTSAPPDFRVFL
                     QEMEMIKGRIAQHNPYIILNISPA"
BASE COUNT          606 a          766 c          600 g          634 t
ORIGIN      
        1 tcatcggtag caaggatgcc ttccccatga tttgccagcg ctgccgaaca ttaaacgctg
       61 aagtcaccaa tgtatgtgtg gagtgtgggc tggacctggg gaagacacca ccggccccag
      121 cccgggtacc cagggcgact gccagatcga aacaagcgat ttccccagct cctggcacca
      181 cggcgctccc tgggttaact tgtccgttgt gtcacacccc tttcagatta tcctggaccc
      241 gctttctcac ctctccctgg agcatctatc aatgcccaca gtgcctcaac cgcttgaaat
      301 tgcgggataa agggctctgg gtgggatttc ttcggatcct gctggttgga attgtgaccc
      361 cgccggttgg aattggattg atgaagtatt cgaacgggga tatcctcatt tcagggattg
      421 cgagtttaat tgtgtgttgt gccatctgtg ttccctgtga caaatatctc aatgaacact
      481 tacgggaatt gacccccctt caatctccac ctccaccaaa aaattaatta tcagaccaat
      541 gcggtatttt atccctctgc aacctcattc atttgaccca gcactgacct tgctgggagg
      601 ccagaccttt cgctggcgct actcgcccga agacaacacc tatcagggga ttgtcaatgg
      661 acaggtttgg cggctctctg tcagttatgg gggaatgcgg gttcatcaag acgaaccgtc
      721 gccaaccgga aaccctccct ttcccattca ggtctttcat tatttcgatc ttcggcgcga
      781 ctatgcccag gcgctggcca tccttcacca ggatccaatc tttttgcagg ccacccgcac
      841 cattaccgga ctcaaactcc tcaatcaaaa ctggtttgag gtcattgtga gctttgtgat
      901 ctcagccaat aaccacatca aacgcatttc cagtattatt gaccggctct gcaccaccta
      961 cggaaccccc atccccggcc agccactggc atgtgcgttc ccaacaccgc aacaacttgc
     1021 cgaggcacaa ctcgacgtat tgcgggaaca atgcggcact ggataccgcg accagtacct
     1081 ggttggactc gcccaacaca ttaccgatca ctcatcccac tggcagcagg caagccagct
     1141 ctcaaccgcc gaattacgcg acctgctgct tggtttacct ggagtcggcc ccaaagtctc
     1201 ggattgtatt ctcttattcg ggtttggccg gtgggatgta ttcccagttg atacctggat
     1261 caggcgtatc atgattgacc tctacttccc aggccaatcc ccttcggatc aggtgattcg
     1321 gcaatttgct ctcgagcagt ttggccagtt ggcagggttg gcccaacaat atctttttga
     1381 agcagctcgc acccagaaag ccacacctgc cagatgaagc gctgccccct ggagttcggg
     1441 gctgcttttt ctgcttgaac gttagccagg tcgggtttaa ccagcttctc ccaattcacc
     1501 tcaccgacac cgatcagtcg gggagaccag acgcaatgtc ggcgcgccga catcagcctt
     1561 tatggccgcg aactgcctgg tttaccgctt tttggatgat ttacttttca cctgcccacc
     1621 ggtatgatga aggctcattt ggtacaagat tcgtcgcccc aaactgcacc ttaactccaa
     1681 cctcctaacc caaacgagtt gaatcccatg caaggtgacc tgacgactat ctgccttcca
     1741 gatttgctgc ggtcaattta tgttgaacga cgaaccggag aactgatctt ctctcaaggt
     1801 gaacaacgca agcagatctt ttttgaactt ggcaatattg tattctcggc cagcaaccag
     1861 gcccaggacc gcattggtga agtcttgctg cggcatggct tgctctcacc acaggatttt
     1921 gaccgcgcgg ttgaagcgac caaaagcggc aaacggtttg gaaaagccct cgttgaaatg
     1981 ggcattatgg gtgaacgcga actcatcacc aatgtcacat ttcaggtcct cgacattatt
     2041 tattcccttt ttgcctggac cactggcagt tatcaattta ttgaaggtga gaatcgagtc
     2101 gcggatgaac tcaaactcaa actcaccacg gcgaggatca ttttggaagg cgttcgccgg
     2161 attgaagact ttgagatcat ccgccgtggg ttaggcgata tcaaccggct ggtggcacca
     2221 tcctcttcaa tgctgctccg gatgcaaacc acttcgctca aaccagtcga gcgaaaggtg
     2281 gtcgacctgg tgacccaacc cactgattta ttgaaaattc tgattacgac tgatgccaca
     2341 cctgaactca ccctcaaatc aatttatggc ttactctcgg ctggaatcct ggaacaaacc
     2401 caggtagccg aactctccca ccgaaccggc aaatttgcgg taccaaatga aattgccgct
     2461 cagccaactc caccgccgcc gccaaccgtg actcgaacca caacctcggc gccaccggat
     2521 tttcgagtct tcctccagga aatggagatg atcaaaggac ggattgccca gcataacccc
     2581 tatatcattc tcaatatctc gccggc
//
LOCUS       DAOQDO010000757         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34032_length_2605_cov_3.115686, whole genome shotgun sequence.
ACCESSION   DAOQDO010000757 DAOQDO010000000
VERSION     DAOQDO010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34032_length_2605_cov_3.115686"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKZ53_12425"
     CDS             <1..706
                     /locus_tag="PKZ53_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ41290.1"
                     /translation="SELRALRAQINPHFLFNALNTIRYFVRIDPEAARSLLLSLSEVF
                     QRALRAGEFVQLRSEISYVEAYLSLEKARLDKRLRVVWVVQGVEGQVQDSPLFEYTVP
                     TLILQPIVENAIIHGVAKKPEGGIVRIGIDQADGHLLLQVSDTGVGMSPERITEILGP
                     DQSKTSSIGLRNVDRRLRVLYGEEFRIEIQSEPKRGTWVQIKLPLETRQAGSFELRGR
                     FDSLPKFRVTEPKMKL"
     gene            917..1690
                     /locus_tag="PKZ53_12430"
     CDS             917..1690
                     /locus_tag="PKZ53_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ41291.1"
                     /translation="MRILIVDDEMPARGELRYILGTLIPEAIFSEATNGEEALEMIER
                     EPIEVVFLDINMPGMNGLATAATMLDGPEPPLIVFATAYDDYALQAFELAALDYIVKP
                     FDERRLVQTVERIKQTLYERALLTRKQTVLREFLVKNEALSEMTKLWAERENEIQVLV
                     DYRNILWIEAEDKMVYLQTSNQERLLVRQTLKDLEARLSKHNFARVHKGYIVNLNYIA
                     EVVTWFSGSYVIRLADKPRTEIPMSRRYAAQLKKVTGLH"
     gene            1870..>2605
                     /locus_tag="PKZ53_12435"
     CDS             1870..>2605
                     /locus_tag="PKZ53_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41292.1"
                     /translation="MVDGQIKTKLLQMLKGEEPETVSDLAPKLARIPSERLIFVLSIG
                     AGLAGMSLKTAKEFLTVAPEVGRLLNTFELKTWGEAGKRLASTNIDAAHDFFVASPET
                     LAALPQSYRLRALTLCSRQASLSTKVAVDCFKIMPQVVGSMGDTPTLQRVFTIANEVA
                     RHSVKHSYDLLRQAPRVTAHLATFDTPDGAMTSRVLELTSAFVHRSGGTAAEFFLALP
                     DAFHDNTRTSTERLLHHTSHFLDRSGG"
BASE COUNT          634 a          709 c          653 g          609 t
ORIGIN      
        1 atcggaactt cgtgccctgc gtgcccagat caatccgcat tttctcttta atgcactcaa
       61 tacgattcgc tattttgttc gcattgatcc cgaggccgcc cgcagcctgt tactgagttt
      121 gtctgaagtt ttccaacggg cgctccgcgc tggtgagttt gttcagcttc gaagcgaaat
      181 cagctatgtc gaagcctacc tttcgcttga gaaagctcga cttgataaac gattgcgggt
      241 ggtatgggtc gtgcagggtg tcgaaggcca ggttcaagac tctccgttgt ttgagtacac
      301 agtcccaacc ttgattctgc aaccgattgt ggaaaatgcc attatccatg gcgtagccaa
      361 aaaaccagaa ggcgggattg tccgcattgg aattgatcag gcggatggtc atttgttatt
      421 gcaggtcagc gataccggtg tgggcatgtc acccgagcga attactgaaa ttctgggccc
      481 agaccaaagc aagacgagtt caattggtct tcgaaatgtt gaccgacgat tgcgggtgct
      541 ctatggcgag gaatttcgaa tcgaaatcca atctgaaccc aaacgcggaa cgtgggtgca
      601 aatcaagctt ccgcttgaaa cacgtcaggc cgggtcgttt gaattgcgtg gccggtttga
      661 ctcgctgccg aaattccggg ttacggagcc gaagatgaag ctttaacgcc tggggctgaa
      721 gacaacgggt tcaataccct gacccgactt cttcagcccg atgtcttcag ccctgagctt
      781 gcgaatcttc agcccaactt cttcagcccg acttcttcag cccgatgtct tcagccctaa
      841 gtttggttta tcttgtcccc aaagtttgat aaaaactcag accaggcact cacccccttc
      901 acaaggcagg aatactatgc ggattttgat cgttgatgat gaaatgcccg cacgcggaga
      961 actccgctac attctcggaa cgctgattcc cgaagccatt ttttccgaag ccaccaacgg
     1021 cgaagaagcg ttggaaatga ttgagcgcga gccaatcgaa gtagttttcc ttgatattaa
     1081 tatgcctgga atgaatggtt tggcaacggc tgccactatg cttgacggtc ctgagccccc
     1141 gctgattgtg tttgccacgg cctatgacga ctatgccctc caggcatttg aactggcagc
     1201 gctcgactac atcgtcaaac cctttgacga gcgccgcctg gttcaaaccg ttgaacggat
     1261 taaacaaaca ctctatgaac gcgccctgct gacccgcaaa caaacggtgc tgcgtgagtt
     1321 cctggtcaaa aatgaagccc tcagtgaaat gaccaagttg tgggccgagc gcgaaaatga
     1381 aatccaggtc ctggttgatt accgaaacat tctctggatc gaagccgaag acaaaatggt
     1441 ctacctgcaa acttcaaatc aggagcggct cctcgtccgt cagacgctca aagacctcga
     1501 agcccgcctt agcaaacaca actttgcgcg agtccataaa ggatatatcg tcaacctgaa
     1561 ttatattgcc gaggttgtga cctggttttc aggttcgtac gtgattcggc tggctgataa
     1621 acctcggact gaaattccaa tgtctcgtcg ctatgcagca caactgaaga aagtgacggg
     1681 attgcattaa tttacggggt tgaagattcg caagctcagg gctgaagaaa acgggctcag
     1741 ggcttggggc aattgaaggg atgaaggatg agggatgagg gatgaaagaa aatcagttct
     1801 tcagcccgac ttcttcagcc caacttcttc agcccatgtt cttcagcccc aggctcttca
     1861 taaaaaacca tggtagatgg acaaatcaaa accaaactcc tgcaaatgct caaaggcgaa
     1921 gagccagaaa ccgtcagcga cctggcgccc aaactcgccc gcattccgtc tgaacgcctg
     1981 atttttgttc tcagcattgg cgccggcctg gccgggatgt cactgaaaac tgccaaagaa
     2041 tttctgaccg ttgcgcctga agtcggtcgg ttgctcaaca cctttgaact taaaacctgg
     2101 ggcgaagccg ggaaacgact ggcctcaacc aatattgacg ccgctcatga tttttttgtc
     2161 gccagcccgg aaacgctggc agccctcccg caaagctacc ggttgcgagc cctgaccctg
     2221 tgttcgcgac aggcatcgct ttcaaccaag gtcgcggtgg attgctttaa aattatgccc
     2281 caggtggtcg gcagcatggg tgatacacca accctgcaac gggtttttac cattgccaat
     2341 gaagttgccc gccattcggt caaacatagc tacgacttgc tccggcaggc gccgcgcgtc
     2401 acggcgcacc tggccacctt tgacacacct gacggcgcga tgaccagtcg tgtcctggag
     2461 ttaacctcag cctttgtgca tcgctcggga ggaacagccg ctgaattctt cctggccctc
     2521 ccagatgcct ttcatgacaa cacccgcacc agcacggaac gactcctcca ccacacatcg
     2581 cattttctag accgcagtgg cgggc
//
LOCUS       DAOQDO010000758         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34059_length_2604_cov_2.654374, whole genome shotgun sequence.
ACCESSION   DAOQDO010000758 DAOQDO010000000
VERSION     DAOQDO010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34059_length_2604_cov_2.654374"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..509
                     /locus_tag="PKZ53_12440"
     CDS             99..509
                     /locus_tag="PKZ53_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41293.1"
                     /translation="MSHSSSSRSVQWKAWLVLGCVFGLGCLTGLSLGGVFTSRAAKPA
                     AVPAPNIRDGEAYFATLQRELNLNPDQAGTIKTIISEAGTEYKAVCTEVRPRYDQVRD
                     RARSRVRVLLSADQQKKFDVLVTQENCNCPELKK"
     gene            748..1200
                     /locus_tag="PKZ53_12445"
     CDS             748..1200
                     /locus_tag="PKZ53_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ41294.1"
                     /translation="MFSKVLTAFAISLTIFVSSSFVAQAAFSPAVLEEKGNVTYTEAD
                     YPRISVDELKTKLAKGEEVIIIDVRGKDFDASATKIKGALRFNGDLKTAAASLPRDKE
                     IVTYCACSTDGGSVKAAMMLREAGFTNVRALKGGWTAWNAAQGAVESK"
     gene            1391..2041
                     /locus_tag="PKZ53_12450"
     CDS             1391..2041
                     /locus_tag="PKZ53_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNJ41295.1"
                     /translation="MSHERTAFTNRITAGQALARKLVSYHNQPGLLVLALPRGGVPVA
                     AEVAKALNAELEVIPVRKLGVPGNSELAMGAIALGGQHVFNQEIIEYLHLTRPILQAA
                     IDRETKELTRRNEIYRQGRPMPDLANRTVILVDDGLATGATMKAAIAAVRAGNAARIV
                     VAVPVGAEETCQSLAQDVDELVCLLKPEPFYGVGFGYKDFSQLTDQQVLDCLPDQS"
     gene            2272..>2604
                     /locus_tag="PKZ53_12455"
     CDS             2272..>2604
                     /locus_tag="PKZ53_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41296.1"
                     /translation="MRPLVLTFLVMLCLFTLNQPSSQLSSNAQSSVTIVLGEAAASSP
                     IRPLLGVNAGPAPVGEPGNPDLTAAYQSAGVTMVRSHDFYGPLDMTTMYPNQAADPGL
                     ASSYDFSAS"
BASE COUNT          618 a          706 c          632 g          648 t
ORIGIN      
        1 atgagggatg aaagaaaacc acttcttcag cccgacttct tcagcccgat gtcttcagcc
       61 cgatgccttc agcccgactt cttcagccct ggaattttat gtctcactct tcttcttctc
      121 gatcagttca atggaaagcc tggctggtgc tgggttgtgt gtttggtctt ggatgcctga
      181 cgggactttc acttggcggc gtttttacct cccgggcggc caaacctgcg gcagtccccg
      241 cacccaacat tcgggatggc gaagcttatt ttgcgacact ccagcgtgaa ctcaacctca
      301 atccagatca ggcgggcacg atcaaaacca tcatcagcga agccggaacc gaatacaaag
      361 ctgtgtgtac cgaagtccgc ccgcgttacg atcaggtccg cgaccgcgct cgctcccgcg
      421 tgcgcgtgtt gctgtctgcc gaccagcaaa agaagtttga cgttctggtt acacaggaaa
      481 actgcaattg tcccgaatta aaaaaataat ttcttttctg tgtaaccaat cgttttttcc
      541 ttccgttaat ggaaagtaag caagtcaaaa atactcaatt tccttcttct tcaattcaaa
      601 aatactgatt gaattttgta tggtttaatc agtacaaatc tgattggttt ctattggaac
      661 cagttgaagg aattgtgttt gtaaaattgc atatttgtgt caaacagttc actcaaccac
      721 acttaaattt tgtaaagagg agcatctatg ttttcaaaag ttctgactgc attcgccatt
      781 tctttgacga tttttgtttc ttcaagcttt gttgctcagg cagcattttc accagcagtt
      841 cttgaagaaa aaggcaacgt tacgtacacc gaagccgatt atccacgcat ttcagttgat
      901 gaattaaaga ccaaactcgc caaaggcgaa gaagtcatta ttattgatgt tcgcgggaaa
      961 gatttcgacg ccagtgccac caagatcaaa ggcgcgctcc gattcaacgg cgatttgaag
     1021 acagccgcgg cgagccttcc cagggacaag gaaatcgtga cctattgcgc ctgctcaacc
     1081 gatggtggtt cggtcaaagc ggcgatgatg ctccgtgaag ccgggtttac caatgtccgg
     1141 gcgttaaagg gcggctggac agcctggaac gcggctcaag gggccgttga aagcaagtag
     1201 ggctgaaaac ttcgggctga agaaaacggg ccgaagatgt cgggctgaaa gcttcggact
     1261 gaagaaaacg gattggggca aacaaaatct gtttctgcta aactgttatc tcagcccaaa
     1321 tttttcagcc cgaattcttc agccccaagc ccaatgtctt cagcccgctg tcttcggccc
     1381 gaagtgttct atgagtcacg aacgaaccgc tttcaccaac cgcatcaccg ccggtcaggc
     1441 actggccaga aaactggtgt cctaccacaa tcagcctgga ctgctggtcc tggccctgcc
     1501 gcgtggcgga gtccctgtcg ccgccgaagt tgccaaagcg ctcaatgctg aactcgaagt
     1561 catccccgtg cgcaaactgg gcgtgccggg aaattctgaa ctggccatgg gggcgattgc
     1621 cctcggcggc cagcacgtct ttaaccagga aatcattgag tatctgcatc ttacacgccc
     1681 cattctgcag gcggcgattg accgtgaaac caaagaactg acccgccgca acgaaatcta
     1741 tcgccagggg cgtccaatgc cggacctggc caatcgaacg gtgattctgg tagatgatgg
     1801 actggccacc ggggccacga tgaaagcggc aattgctgcc gttcgggccg gaaacgctgc
     1861 ccggattgtg gttgccgtcc cggttggtgc cgaagaaacc tgccagagtt tagctcaaga
     1921 cgtagacgaa ctcgtgtgcc tgctcaaacc tgaacccttt tacggcgtcg ggttcggata
     1981 taaagatttc tcacaattga ccgatcagca ggtgcttgat tgtttaccag atcaaagctg
     2041 agaccatttt gcagtgaaat tatcgtatta aaggaaagct aaattattga aagagttgta
     2101 tttccttcag ccctgagccc gataccccca gcccaagatc gtctgaccct cgccattcca
     2161 ggcaaaacac aatttccgct actttttcag actttttcaa ctggtcgctt cgtctgaaca
     2221 cctgttcgtc ccttctcatc attcccatcc cactgcaaag agtttcggcc tatgcgacca
     2281 ctggtgttga ccttccttgt gatgttgtgt ctgttcacac tcaatcagcc ttcctcccag
     2341 ctttcatcga atgcccaatc atccgtcacc attgttttgg gtgaggcggc ggcatcatcg
     2401 ccaattcggc ccttgcttgg cgtcaatgcc gggccggcgc cggttggcga gcctggaaac
     2461 cctgatttga cggctgccta tcagtcagcc ggcgtcacga tggtcagaag ccacgatttt
     2521 tatggcccgc tcgatatgac gacgatgtac cccaatcagg ccgccgaccc aggtctggcg
     2581 tcgtcttatg acttcagcgc cagc
//
LOCUS       DAOQDO010000759         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34100_length_2602_cov_3.182175, whole genome shotgun sequence.
ACCESSION   DAOQDO010000759 DAOQDO010000000
VERSION     DAOQDO010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34100_length_2602_cov_3.182175"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..360
                     /locus_tag="PKZ53_12460"
     CDS             172..360
                     /locus_tag="PKZ53_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41297.1"
                     /translation="MSKSKFVGIALMFSGLIFWGLAFAVNQGIILGGSNPRLIFLAFC
                     AVGAFDFLIGLAIFFLKR"
     gene            493..2541
                     /locus_tag="PKZ53_12465"
     CDS             493..2541
                     /locus_tag="PKZ53_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNJ41298.1"
                     /translation="MVYPPAKKVDQVDDYHGTKVPDPYRWLENPDSEETRAWVEAENK
                     VTFGFLEEIPSRARIKERLTKLWNYEKYGVPAKFGDRYIYSKNNGLQNQNVLYTTTSL
                     GGQAQVLLDPNTLSTDGTVALSNYEVSEDGKLFAYGLSASGSDWMEWRVRDIATGKDL
                     PDLIKWVKFSSVSWLKDASGFFYSRYDEPNESTKLQSVNYFQKLYFHKLGTSQSDDVL
                     VYERPDEKEWQFEGTVSEDGKFLIITVTQGTSPNTRVFYKELSNGNGKVTELLNKYDA
                     SYEFLGNDGAIFWFKTNQDAPRGRVIAIDTQNPQPESWKELIAEAPETMQSVSVVNNQ
                     FVVNYLKDARTQVKLFQLDGKFDREVTFPGIGTATGFKGKRQDSDTFYSFTSFTTPTT
                     IYRYDLKTGKSSVFRQPKVGFKPGLYETKQVFYTSKDGTKVPMFISHKKGLKLDGNNP
                     TVLYGYGGFNISLTPTFSVSNLVWMEMGGIFAQPNLRGGGEYGEEWHLAGTKLRKQNV
                     FDDFISAGEWLIANKYTSTPKLAIYGGSNGGLLVGACMTQRPDLFGACMPAVGVMDML
                     RFQKFTIGWAWTSDYGSSDNPEEFKALYAYSPLHNIKPGTAYPPTLVTTADHDDRVVP
                     AHSFKFAAALQAAQSGPNPVLIRIDTKAGHGAGKPTAKIIDEAMDRFAFLVRVLNIKM
                     "
BASE COUNT          685 a          624 c          623 g          670 t
ORIGIN      
        1 aatggtccgg gattccctgg tggcagccgg attccgaact gaccggcact gatatgtgag
       61 caagataatg ctattttggg tgaaattgag gtcattcttc attctcaatt cttcattctt
      121 cattcttcat tcttcattct caattcttca ttcttcattc ctgagttact tatgagcaaa
      181 tcaaaatttg tcggcattgc attgatgttt tcagggttga ttttttgggg actggctttt
      241 gcagtcaacc agggaataat tcttggtggg agtaaccctc gcctgatttt tctggctttt
      301 tgtgccgttg gcgcttttga cttcctgatt ggacttgcca ttttcttctt aaaaaggtga
      361 tagtccaggt ttcttctatt tacatttttg aagcacttct ctgcccgaat aaccagtttg
      421 atcctggtac tcgggttgtt tgctccactt ctttcttccc ctgcttattc ttcaccccaa
      481 tccaacaaat ccatggtgta tccaccagct aaaaaagtag atcaggttga tgattaccac
      541 gggaccaaag tcccagatcc gtatcggtgg cttgaaaatc cagactcaga agaaacacga
      601 gcctgggttg aagccgaaaa caaagtgacc tttgggtttc ttgaagaaat tccgtcacgc
      661 gccaggatca aagaacgcct gaccaaactc tggaactatg aaaagtatgg ggttccagcc
      721 aaatttggtg atcggtatat ctattcgaaa aacaatggat tgcaaaacca gaacgtcctg
      781 tatacgacca cgtcgctcgg cggacaggcg caggtactgc ttgaccccaa cacgctttca
      841 actgacggaa cagtggcact gtccaattat gaagtcagcg aagacggcaa attgtttgcc
      901 tatgggctgt cagcctccgg gtcagattgg atggagtggc gggttcgcga tattgccacc
      961 ggcaaagacc tcccggattt gatcaaatgg gtcaagtttt catcggtttc ctggttgaaa
     1021 gatgcctccg gtttcttcta cagccggtat gatgaaccaa atgaaagcac caaactgcaa
     1081 agcgtcaatt acttccaaaa gctctatttt cataaacttg gcacgtcaca gtcagacgat
     1141 gtcctggtct atgaacgccc agacgaaaaa gagtggcagt ttgaaggaac cgtttcagaa
     1201 gacggcaaat tcctgatcat caccgttacc cagggcacca gccccaatac cagggtgttc
     1261 tataaagaat tgtccaacgg aaacggcaaa gtcaccgaac tgctcaacaa atatgatgca
     1321 tcctatgagt ttttaggcaa tgacggcgcc atcttctggt ttaagaccaa ccaggacgcg
     1381 ccgcgaggtc gggtgatagc cattgatacc cagaatccgc aacctgaaag ctggaaggaa
     1441 ctgattgccg aagctcctga aaccatgcaa tcagtctcag tggtcaataa ccagtttgtg
     1501 gtcaattatt taaaagatgc ccgaacccag gtgaaattat tccaactgga cggcaaattt
     1561 gatcgcgaag taacctttcc agggattgga accgccactg ggtttaaagg gaaacggcaa
     1621 gacagcgata ccttttattc atttaccagc tttaccacac caaccaccat ctatcgctat
     1681 gacttgaaaa ccggcaaaag cagcgttttc cgtcagccga aagttgggtt caaacctggt
     1741 ttgtatgaaa ccaaacaggt gttttacacc agcaaagatg gcaccaaggt gccgatgttc
     1801 atcagccaca aaaaaggctt aaagcttgat ggaaacaacc caacagtcct ctatggctac
     1861 ggcggattca atatctcact gacacccacg ttttcagtgt ccaacctcgt gtggatggaa
     1921 atgggtggga tttttgcaca accgaacctc cgtggtggtg gcgaatatgg cgaagagtgg
     1981 catctggccg gcaccaagtt gcgcaaacaa aatgtgtttg atgattttat cagtgctggt
     2041 gaatggttga tcgcgaacaa atacacctct acaccaaaac ttgccattta cggaggcagt
     2101 aatggtggct tactcgtcgg cgcctgtatg acccagcgcc cagacctgtt tggtgcctgt
     2161 atgccggcgg tcggggtgat ggatatgctt cgctttcaga agtttacgat tggctgggcc
     2221 tggacctcag attatggttc atcagacaat ccggaagaat ttaaggcgct gtatgcctat
     2281 tcaccattgc acaatatcaa gccgggcaca gcctatccgc caaccctggt gaccacggct
     2341 gaccatgacg accgggtcgt tccggcgcat agcttcaaat ttgctgccgc tctgcaagcc
     2401 gcccaatcag gaccaaatcc ggttttgatc cgcattgaca ccaaagccgg acatggcgct
     2461 ggcaagccaa cggccaagat cattgacgaa gccatggatc ggtttgcctt cctggtgcga
     2521 gtgctcaata tcaagatgtg aggattgggg ctgaagaagt tgggctgaag aagttgggct
     2581 gaagaagttg ggctgaagaa gt
//
LOCUS       DAOQDO010000760         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34163_length_2600_cov_2.739489, whole genome shotgun sequence.
ACCESSION   DAOQDO010000760 DAOQDO010000000
VERSION     DAOQDO010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34163_length_2600_cov_2.739489"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1990)
                     /locus_tag="PKZ53_12470"
     CDS             complement(<1..1990)
                     /locus_tag="PKZ53_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNJ41299.1"
                     /translation="MLTSIFATLLMSFLPFGPQSNEKAATPQPGVPIELAQHRASHYH
                     KVQYLLTLNLTPNSEKLTGTAEILLTLDKDVPELVLDWRPQTFADQSQARVWNIEVND
                     QPVSDARFENEHLIIPKTYLESGINRVSLKFESAVKRSGSAVTRYVDQQDKSEYIYTL
                     FVPSDASTLFPCFDQPDLKARFILELTVPEDWNVVSNTDVAWSNVETESNRLRVRFHK
                     TEPISTYLFAFAAGPFEILQDSKVKDPTRLFVRKSKMARARQDADEVLRLNREAVLYF
                     ESYFKYRFPFSKYDLVLIPEFPYGGMEHAGATFLRENAILFLSTPSGNDLVGRAQLIF
                     HETAHQWFGDLVTMKWFDDLWLKEGFANFMAAKATEKILPQYNAWNAFHAAKTNAYRT
                     DITPGTTPIYQVIPNLSAAKSAYGNIVYNKAPSVLRQAEFYLGAKVFQTAVQQFVKQH
                     AFANAEWKDLVHSFEQASKRELDTWADAWVKRRGLANIRAGWELDQHQRIRNFTLTQE
                     NALGEAGVWPMRLQILAHWRNDQTKTFPVILDGPKTVVSELNGLPEPLFVYANAGDYG
                     YGRFLLDVHSRKSLLTEFQHVEDPFLRSLLWGALWDAVLDADLAPRDYLNLVLANLPI
                     EQDEVTISALLGRTFTLCEKYLSPAQRQQMAEPVEKMLT"
     gene            complement(2065..2520)
                     /locus_tag="PKZ53_12475"
     CDS             complement(2065..2520)
                     /locus_tag="PKZ53_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41300.1"
                     /translation="MTNYIPLTPETFFTARQFVLDRWRARARELGRPEPLDLSRACLF
                     ASLFAYQVFGGTLQGNRFHQYTIKSGHLIDLTIGSEDLQRLREAGIDPHEHDPRWWAN
                     PVHLEAMTSCLPRVERWVEEFLMEMNAEDGEFQPPQFSEPNRDLNSYRR"
BASE COUNT          652 a          607 c          689 g          652 t
ORIGIN      
        1 ctgtcagcat tttttcaacc ggttcggcca tctgctggcg ttgggccgga gacaggtatt
       61 tttcacagag ggtaaacgtg cgtccgagca aggctgaaat ggtcacttca tcctgttcaa
      121 tcggtagatt ggccagaacc aggtttaagt agtcacgcgg tgccaggtca gcatccaaaa
      181 ccgcatccca gagcgctccc cagagcaacg accgtaaaaa tgggtcttca acgtgctgaa
      241 actcagtcag gagcgatttt cgactgtgca cgtcaagcaa aaatcggcca tacccataat
      301 caccagcatt cgcatacaca aaaagcggct ctggcaagcc gttcaactct gaaactaccg
      361 tctttgggcc atctaaaatc acagggaagg ttttggtctg gtcgttacgc cagtgggcca
      421 ggatttgaag ccgcattggc cacacaccgg cttcacccag cgcattttcc tgagtgaggg
      481 taaaattgcg gatccgctgg tgttgatcga gttcccagcc agcgcggata ttggccagtc
      541 cacggcgttt tacccaggca tcggcccagg tgtcgagttc acgttttgaa gcctgttcaa
      601 atgaatggac cagatctttc cattccgcat tggcaaaagc atgctgtttg acaaattgtt
      661 gaactgcggt ttgaaagact tttgctccca gataaaactc agcctggcgc aacacgcttg
      721 gcgccttgtt gtagacaata tttccataag ccgatttggc cgctgaaagg ttgggaatta
      781 cctgataaat tggcgtggtg cccggtgtga tgtcagttcg ataggcattg gtcttggcgg
      841 cgtgaaaggc attccaggca ttgtattgcg gcaggatttt ttcggtggct ttggcagcca
      901 taaaattggc aaagccttct ttgagccaca aatcatcaaa ccacttcatg gtgaccagat
      961 caccaaacca ctgatgagcc gtttcatgaa agatcagttg tgctcgtccc accaggtcat
     1021 ttccactggg cgttgagaga aatagaatcg cattttcccg caggaaggtc gctccggcgt
     1081 gttccatccc accataagga aattcaggaa tcagcaccag atcatatttg gaaaacggaa
     1141 accggtattt aaagtagctc tcaaagtaca gtactgcttc gcgattcagg cgcagcactt
     1201 cgtcggcatc ctggcgggca cgcgccattt tggattttct gacaaacagt cgagtggggt
     1261 ctttgacttt ggaatcctgc aatatttcaa atgggccggc ggcaaaggca aacagatagg
     1321 tactgatggg ttcggtttta tgaaatcgga cacggagccg atttgattcg gtttccacgt
     1381 tggaccaggc cacgtcagtg ttggaaacaa cgttccagtc ttccgggacc gtgagttcga
     1441 ggataaatcg ggctttcaaa tcaggttgat caaaacaggg aaacagggta ctggcgtcag
     1501 atggaacaaa cagcgtgtag atatattccg atttgtcctg ttgatctacg tagcgtgtca
     1561 cggctgatcc ggaccgtttt accgctgatt caaacttcag tgagactcga ttgatgccgg
     1621 attcaaggta ggtttttgga atgatgaggt gttcattttc aaatcgggca tcggaaacag
     1681 gctgatcgtt gacttcaata ttccagactc tggcctgact ctggtcggca aatgtttgtg
     1741 gtcgccagtc aagcaccagt tccggcacgt ctttgtccag tgtgagcaga atttccgcgg
     1801 tgccggtcag tttttctgag tttggtgtca gatttaaggt cagtaaatat tgaactttat
     1861 ggtagtggga tgcgcggtgt tgagcgagtt cgattggaac ccccggttgc ggagtagcag
     1921 ccttttcatt tgattggggt ccaaatggaa gaaatgacat gagcaaggtc gcaaagatgg
     1981 aagtcagcat tgattgaggt tttatttaaa cttatcagac tcaaaaattc aatattggcc
     2041 ggtgaagccc cgtctgaact ggctttaccg acgatagctg tttaaatccc ggtttggttc
     2101 agagaattgc gggggttgaa attcgccatc ctcagcgttc atttccatca ggaactcttc
     2161 aacccagcgt tcaacccgag gtaaacacga cgtcatggct tccaaatgca ccgggttggc
     2221 ccaccagcgt gggtcatgtt cgtgtgggtc aatgccagct tcgcggagcc tttgtaaatc
     2281 ctcggatcca atcgtcaaat caatcagatg cccggacttg atggtgtatt ggtggaagcg
     2341 attcccttgc aacgttcccc caaaaacctg gtaggcaaac aaggaggcaa ataaacaggc
     2401 ccgtgaaaga tccaagggct cgggacgacc aagctcgcgt gcccgggcgc gccagcggtc
     2461 taaaacaaat tgacgagcag tgaaaaatgt ttctggtgtc agtgggatgt aattggtcac
     2521 gggatgaagt cctatcaaac aacatcagac aatttccctg gatagaggaa catgcggcga
     2581 gaaagccaaa gccggggatg
//
LOCUS       DAOQDO010000761         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34180_length_2599_cov_2.704403, whole genome shotgun sequence.
ACCESSION   DAOQDO010000761 DAOQDO010000000
VERSION     DAOQDO010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34180_length_2599_cov_2.704403"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKZ53_12480"
     CDS             <1..485
                     /locus_tag="PKZ53_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNJ41301.1"
                     /translation="RLEALSPPLAGVWSRLYQTGWLPLVQGIQSLTPGRHLALAGVLS
                     GVYLSTQVVAFWGALRACQIQVALPTALLVFLLVQLGTMLPNTPSNIGTYQFFCTLGL
                     TLVGIEKNAAVAASMLVFLILTVPLWILGCIATSQCQLSGAQLNQIASQQATEAPDAI
                     "
     gene            574..>2599
                     /locus_tag="PKZ53_12485"
     CDS             574..>2599
                     /locus_tag="PKZ53_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ41302.1"
                     /translation="MSKFFACYQPGSSHSFVHLFAETLHSRSLAEHILLTSDYCHITP
                     PDRLPQVLVIAAVTVPWLTQAVTSFPGNEIIVFVPPDLDPMAVRLMAEGQIVDIVHTD
                     RPARLPWAIKSRTPRTKETLAGGTPVQHISSTESPIDSSFAQVNVSGKDQLRPPLHQT
                     IESIPGILYRYQVFPDGSNRYEYISPGVKHLLGLEPEDVLKDPMVFQSRLLPDSVRFL
                     VQKRYDMLRNPSDWSGEFEVFNHQGTKVTLQAHASPMRQPNGSLIWHGILIDVTEKER
                     LTEQLLENESIRRVFFDNAPFRLGVIELTPDEDIQFILVNSRFAQLCGKTPEEIIGKK
                     TTELGTSQESLRKWVQAYRQAQSTGQLAKIDMFEADTNRWVQGVVAQIPSSSILNPRF
                     LFIIEDVTEHRQSIERLRLLESAVERANDVILITEAEPINLPGPRIVYVNPAFERMTG
                     YTAQEAIGNTPRMLQGPKTSDETRERIVRHLKEWRSVEVEVLNYRKDGTEFWAELNIV
                     PVADETGWYTHWVAIQRDITARKMAELALRESESRFRILADTAPVMIWVTNEAGQTTW
                     FNRQWMEFTGKPLESHLGTGWKECLHPDDQGKLLGTCQQAALDQIKFQAECRLKRFDG
                     QYRIMLDVGVPRFLETGQYVGHIGTCIDITDVKQAEAERGQVARLESLGTL"
BASE COUNT          618 a          685 c          658 g          638 t
ORIGIN      
        1 ccaggctgga agccctgtca ccgcccctgg ccggagtctg gtccaggctg tatcaaaccg
       61 gatggcttcc tctggtccag gggattcagt cactgacacc cggacgacat ctggccctcg
      121 ccggtgtgct ttccggcgtg tatttatcaa cccaggttgt ggctttttgg ggggcgcttc
      181 gggcgtgtca gattcaggtt gccctgccga cggctctgct cgtttttctg ctagttcagc
      241 ttggaacgat gctcccgaat acaccttcaa atattggaac ctatcaattt ttttgcaccc
      301 tgggcctgac acttgttgga atagagaaaa acgcggcggt ggccgcgtca atgctggttt
      361 tcctgatttt gacggttcca ttatggatcc tgggttgtat cgccaccagt cagtgccagc
      421 tttcaggtgc tcaactcaac cagattgcat cccaacaggc aactgaagca ccggacgcaa
      481 tctagctgca gagatggtaa ttttgtggaa catatggtgt aatgagcggt tcttgccctc
      541 gatctgacct cttgccgcaa tcgcaccttg cccatgtcaa aattctttgc gtgctatcag
      601 cctggtagct cccactcttt cgttcatctt tttgcagaga cgcttcactc ccggtccctc
      661 gccgaacaca tacttctgac atcagattat tgccacatta cgcctccgga taggcttccc
      721 caggtactgg ttattgcggc agtgaccgtg ccctggttga cccaggccgt taccagcttc
      781 cctggtaacg aaatcattgt atttgttcct ccagaccttg atccgatggc cgttcgactg
      841 atggctgaag gtcagattgt tgatattgtc catactgacc gaccagccag attgccctgg
      901 gccatcaaat ccaggacgcc ccgcacgaaa gagacactgg cgggtgggac cccagtgcaa
      961 cacatcagtt caaccgaatc acccattgat tcgtcatttg ctcaggtcaa tgtgtcaggc
     1021 aaagaccagc tccgcccccc tcttcatcaa accatcgaat ccattcccgg cattctctat
     1081 cggtatcagg tgttcccgga cgggtctaac cggtatgagt atatcagccc tggggtcaag
     1141 cacctgctgg ggctggaacc agaggatgta ctgaaagatc cgatggtatt tcaatcccga
     1201 cttcttccgg attcagtccg atttctggtt caaaagcggt atgacatgct tagaaatccg
     1261 tcagactggt ctggtgaatt tgaggttttt aaccaccagg gcaccaaagt cacactccag
     1321 gcacatgcct ctccaatgcg ccagccaaac ggttcactga tctggcatgg gattttgatt
     1381 gatgtaactg aaaaagaacg cctgaccgag caattgcttg aaaacgaatc aatccggcgt
     1441 gttttttttg ataatgcgcc gttccggttg ggcgtcattg aactcacacc agatgaagat
     1501 attcaattta tcctggtaaa cagtcgtttt gcccagcttt gtggaaaaac cccggaagag
     1561 attatcggga aaaaaacaac tgaattgggc acctctcagg aatcactccg aaaatgggtt
     1621 caggcgtacc gacaggcaca atcaaccggg caactggcca aaattgatat gtttgaagcc
     1681 gatacgaacc gctgggttca gggcgtggtg gcgcaaattc catcctcctc aatcttaaac
     1741 ccacgatttt tatttatcat cgaagatgtc accgagcacc ggcagtccat cgaacgcttg
     1801 cgattgctcg aatcagccgt tgagcgggcc aatgatgtga tcctgattac tgaagccgaa
     1861 ccgattaacc ttcccggccc tcggattgtg tatgtaaatc cggcgtttga acgcatgacc
     1921 gggtacacgg ctcaagaagc aattgggaat actccccgga tgctgcaggg accaaaaaca
     1981 tccgatgaga cacgagaacg aattgtcagg catttgaaag aatggcggtc tgtcgaggtt
     2041 gaagttttaa attaccgcaa agatggcacc gagttttggg cagaactcaa tattgtgcct
     2101 gtcgccgatg aaaccggctg gtatacccat tgggttgcca tccaacgtga tattacagcc
     2161 cgaaaaatgg ccgaactggc actccgcgag agcgaaagcc ggtttcggat tttagccgac
     2221 accgcacctg ttatgatttg ggtcacgaat gaagccggtc aaaccacctg gtttaatcgt
     2281 caatggatgg aatttacagg taagccgctt gaatcccacc tcggaactgg gtggaaagaa
     2341 tgcctgcacc ctgatgacca ggggaaactc ctggggacct gtcagcaggc ggctcttgac
     2401 cagataaagt ttcaggctga atgccggctc aaacgatttg acggtcaata tcgcatcatg
     2461 ctggacgttg gggtacctcg gtttttagaa accggccagt atgtcgggca catcggcacc
     2521 tgcattgaca ttacggatgt caaacaggct gaagccgaac gtggtcaggt ggcccggttg
     2581 gaaagcctgg gaacactgg
//
LOCUS       DAOQDO010000762         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34217_length_2597_cov_3.704957, whole genome shotgun sequence.
ACCESSION   DAOQDO010000762 DAOQDO010000000
VERSION     DAOQDO010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34217_length_2597_cov_3.704957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1272
                     /gene="lpxC"
                     /locus_tag="PKZ53_12490"
     CDS             361..1272
                     /gene="lpxC"
                     /locus_tag="PKZ53_12490"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNJ41303.1"
                     /translation="MRPQTTLARAVHTTGIGLHTGTPVELTIFPAPPDTGYVFRRADL
                     NGFELSATPHHVAHVSYATTLMRKGVMVSTVEHLLSALHGCQVDNAIIELNSFEVPIL
                     DGSALPFVELIEAASVVELPAIRQTLRILRSVEIIDGARRIAIHPYQGFRIESTIDFP
                     HPVIGRQTSALDLTPENYRREIAPARTFGFIHEAEALRKSGLVRGASLKNAVVLDKEK
                     VLNPEPLRFSDEFGRHKVLDIVGDLALLGMPIEGRIVAERSGHGLHSALMAKLIRETD
                     AWEVTEADVDLPAILAANGFFREAVRA"
     gene            complement(1367..1750)
                     /locus_tag="PKZ53_12495"
     CDS             complement(1367..1750)
                     /locus_tag="PKZ53_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ41304.1"
                     /translation="MSLTDKYSSVYNLAQQLGCDPLSATEDGGKLVLKANAPHQYAAN
                     QLWDAIKEIDPNLNHGDLVFDWSVARDDIFGEYTVKSGDTLSGIAKKVTGNKLSYQQI
                     FEANRHILNDPNKIQVGQKLTIPKH"
     gene            1969..2511
                     /gene="cobO"
                     /locus_tag="PKZ53_12500"
     CDS             1969..2511
                     /gene="cobO"
                     /locus_tag="PKZ53_12500"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNJ41305.1"
                     /translation="MNTKRTPLVMINTGNGKGKTTAALGLLMRAAGRNFKTIMLQFIK
                     TTEDPRFGVYGEILSAHKLGIEILSLGDGFTWDTKNPDQDKQTARKAWEICVEKINSG
                     VYDIVALDEITYAFHFGWLTVQEVIDVLKNRPANLHVVLTGRNAPPELIEFADLVTEM
                     TEIKHPFTEKKVVAQLGIEF"
BASE COUNT          652 a          665 c          621 g          659 t
ORIGIN      
        1 gaaccaagaa ccagttttct tcattcttca ttcttcattc ttcattcttc attctgttga
       61 cttcctgcca caccaaatgt ccacctttgt ggcagaacca caacaggcag ggatcagaga
      121 tttttgagac ttctgagaag tgcctgtatt tggcgagttt gcaaattttt ggcatgagtt
      181 caaattttgg tatgcaatat gctgggcttt caacggtttg tatttttcat tccaatcaca
      241 atctaaagga acgagcgaaa ggatccatcc agagattttg ccagggaaac gtcttcccaa
      301 gttttttcaa ccaagcccca ctgcccatga aaaaacctgg ctccagctcg tgaggaatca
      361 atgagaccac aaacgacgct tgctcgcgcc gtccatacca ccgggattgg tttacatacc
      421 ggtactcctg ttgaactgac cattttccca gccccacccg ataccgggta tgtttttcgc
      481 cgggctgacc tcaacggctt tgaactctcc gcgacgcccc atcacgtagc ccacgtcagt
      541 tacgccacca ccttgatgcg caaaggcgtg atggtctcca ccgtcgaaca tctcctttcc
      601 gcattgcacg gctgccaggt tgacaacgca atcattgaac tcaacagttt tgaagttccg
      661 atcctggatg gcagcgcgtt gccgtttgtc gaactcatcg aagccgcctc agtcgttgaa
      721 cttcctgcca ttcgacaaac gctccggatt ttgcgctcag ttgaaatcat tgacggcgcc
      781 cgccgaattg ccattcatcc gtaccagggt tttcggattg aaagcacgat tgattttccc
      841 caccccgtga ttggtcgtca gaccagtgcc cttgacctca ctccagaaaa ttatcgccgt
      901 gaaattgctc ccgcccgaac ttttgggttc atccacgaag ctgaagccct tagaaaaagc
      961 gggcttgtgc gtggcgcgtc actcaaaaac gccgtcgtac tcgacaaaga aaaagtcctg
     1021 aatccagagc cattgcggtt tagcgatgaa tttggacggc ataaagttct tgatatcgtt
     1081 ggagatctgg ctttattggg aatgccgatt gaagggcgaa ttgtcgctga acgctctggt
     1141 cacgggcttc attctgccct gatggccaaa ctgattcgcg aaacagacgc ctgggaagtc
     1201 accgaagccg atgttgacct gccagccatt ctggccgcaa atggcttttt ccgcgaagcc
     1261 gttcgggcct gaagtgctgg gtaaaagctt atttggcaaa tgaaaagggc aggtagttga
     1321 gaaactacct gccctttcta tttcgcgact ttgctatttc actccctcag tgcttgggaa
     1381 tcgtcagttt ttgtccaacc tggatcttat ttggatcatt caggatatgg cgattcgctt
     1441 cgaagatttg ctggtaggag agtttgtttc cggtcacttt tttggcaata ccagagaggg
     1501 tatcacctga cttgacggtg tattcaccga aaatgtcatc acgggcaacc gaccagtcaa
     1561 ataccagatc accgtgattg aggttagggt caatttcttt gatcgcatcc cagagctggt
     1621 tggctgcata ttgatgcgga gcattggcct tgaggaccag tttgccgccg tcttcagtcg
     1681 cgctcagcgg gtcgcaccca agttgttggg ccaggttgta cacactgctg tatttgtcgg
     1741 tgagtgacat acttcaaaaa gctccttaaa taggtgtgat tttgaaaagg gaagtggtaa
     1801 aagatccacc ttgatcaaac gggaggccaa ctctagctgg ttcttgggtt gagatcaacc
     1861 tgtcatattt cacaaaacac caaagcggcg tcaagccgcc gcactccaaa gcggcgtcaa
     1921 gccgccgcac tccaaagcgg cgtcgagccg ccgcactcca cataatatat gaacaccaaa
     1981 cgaacaccgc tggtgatgat taacaccggt aatggaaaag gcaaaaccac ggcggcactt
     2041 gggcttttga tgcgtgccgc tgggcgcaac ttcaagacca tcatgctcca gtttattaaa
     2101 accaccgaag acccacggtt tggcgtctat ggcgaaattc taagcgccca caaactggga
     2161 attgaaattt tatcactggg cgatggcttc acctgggata ccaaaaatcc tgaccaggac
     2221 aaacaaaccg cccgcaaagc ctgggaaatc tgtgttgaaa aaatcaattc cggggtctat
     2281 gacattgtgg cgctggacga aattacctac gcgtttcact ttggctggct caccgtccag
     2341 gaggtcattg acgtcctcaa aaaccgtccg gcaaatctgc atgttgtctt gaccgggcgc
     2401 aacgccccgc cagaactgat tgaatttgct gatctggtga ctgaaatgac cgaaatcaaa
     2461 catccgttta ctgaaaagaa agtcgtggct cagttgggga ttgagtttta acgaatgaag
     2521 aattgagaat gaagaatgta tctggtgctt ggtgcttgga ccaatgtttt cgaagaacca
     2581 agaaccaaga accaaga
//
LOCUS       DAOQDO010000763         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34257_length_2596_cov_2.355765, whole genome shotgun sequence.
ACCESSION   DAOQDO010000763 DAOQDO010000000
VERSION     DAOQDO010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34257_length_2596_cov_2.355765"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKZ53_12505"
     CDS             complement(<1..677)
                     /locus_tag="PKZ53_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ41306.1"
                     /translation="MTLIFQSLPLTRTSADFTSRAFPGAGRTPARQQATLRIRERNPV
                     VNETKQLTLTAVDTSDQPLTGVTWESGSPDIASVDPQTGVVRGVKQGFATVTARRDGE
                     SFSTFVVVARVRNGNGAKVPGDTKTDTGGRIYISDPTGSVILRKGSFAESASIFAGQR
                     GETGVADGQRLSARFNAPTAVTVDNSARGGVYVADTANHKIRKIDFNDQVTTILGSNS
                     PGLMTADV"
     gene            complement(1093..>2596)
                     /locus_tag="PKZ53_12510"
     CDS             complement(1093..>2596)
                     /locus_tag="PKZ53_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41307.1"
                     /translation="ADFFNSRVRRINASSLIIDTFAGNGGSGLIGDGGPATQAEIADV
                     TGVAIDGTGNLFLTDVGSDRVRRVDAQTSTINTVAGSGRQTFQGDGGPANRAGLYIPR
                     AVTLDQTGNLFISDQGNGYIRRVDATTGSIQSIAGDGDTFGGGADGDLALESSILNCF
                     GVAVDPTGENLYLTLGDGGRVWRVTNGRVTTVAGGGQNGLGDGGPATQAQVFDPVGVH
                     LDAAGNLLITELTSHRIRRVNVQNGIITTVAGSGPIGHDSGSFSGDGGPATSALLNLP
                     SAVRTDAAGNLLIADTLNFRIRRVAAQSGTISTIAGGGNDDLGENVPATQVGLLPWDL
                     ALDAAGNIFVAGQDRIRRIDARTGIITTLAGVFQSPGYSGDGGPGTVAQVQDVRSLAF
                     DREGNLYFADVEANVIRVLKGVGVAAPSIAISNATYTRPTLTITGSGFGSTSARVSIN
                     GTDVSRFLKTQTDTTLTLKGNQKKLKLVKGSNQIVVTANGVTSNTFVLPL"
BASE COUNT          643 a          730 c          649 g          574 t
ORIGIN      
        1 acatcagccg tcatcaatcc cggtgaattg ctccccagaa tggtcgtcac ctgatcattg
       61 aaatcaattt tgcggatttt gtggttggca gtatccgcca catagacgcc gccacgcgcg
      121 ctgttatcca cggtcactgc cgtcggagcg ttaaaccggg cgctcaatcg ctggccgtcc
      181 gccaccccgg tttcaccacg ctgaccggca aaaatagagg ctgattcggc aaaactgcct
      241 ttgcgcagga tgacgcttcc ggtcgggtcg ctgatgtaga tgcgtccgcc agtgtcggtt
      301 ttggtatcgc cgggcacttt ggcaccgttg ccgttccgaa cacgtgccac aaccacaaag
      361 gttgaaaagc tttcgccatc acgtcgagcc gtcacggtgg caaatccctg tttgacacca
      421 cgcaccacac cagtttgtgg gtcaaccgag gcaatgtcag ggctgccgga ttcccaggtt
      481 acgccagtca acggctggtc gctcgtatcc actgccgtga gggtcagttg tttggtttca
      541 ttcaccaccg ggttgcgttc gcggatgcga agggtggctt gttgtctggc tggagtccga
      601 ccagcaccgg gaaaagctcg tgatgtgaaa tcagcactgg tgcgagtcag ggggagagat
      661 tgaaatatga gggtcaaaat cagactgaaa tagagaattc tcaaagagga tcggcacata
      721 taaaacctct aacatcataa gtttagggcg gggcaataga gaccggcaaa ataagaagaa
      781 ccgtaaaaaa agctcaatca gggtcaggtg cgaaactcag gttcaaaagg caggctccgc
      841 accaggagat gtcgaaaaat caaaataaaa aagtgtaatt gttttaagga aaatgccggg
      901 ccagaatagc ggagccagaa tctgatgtga acctattttt tgaaagtagt gaacgcaact
      961 gattggaaaa tcctcagaac gcattttcac cacagaggac acagaggacc acagaggatt
     1021 tgactgattg aattcctccg tgttcccctg tgtcctctgt ggtgagctta tttgtccagt
     1081 accggttttg gattacaacg gcaacacaaa tgtatttgaa gtcaccccat tggcagtaac
     1141 cacgatctga ttgcttccct tcaccagttt cagctttttc tgattgcctt tcagtgtcag
     1201 ggtggtgtcg gtttgagttt tgagaaaccg gctgacatcc gttccattga tactgactct
     1261 ggcgctggtt gaaccaaacc cactcccggt aatcgtcaaa gtaggcctgg tataggtcgc
     1321 gtttgaaatc gcgatagaag gtgcggccac accgacacct ttgagcaccc gaatcacatt
     1381 ggcttccaca tcggcaaaat acagattgcc ttcgcggtca aaggcgagac tgcgcacgtc
     1441 ctgcacctga gcgactgtcc caggtccacc gtctccgctg taccctggcg attgaaagac
     1501 cccagccagc gtcgtaataa ttccggttcg ggcatcaatt cggcgaattc gatcctgacc
     1561 ggcgacaaag atgtttccgg cagcatccag agccagatcc cagggcaaca atccaacctg
     1621 ggtggccggg acattttcac caaggtcgtc gttccctccg ccagcaatcg tcgaaatagt
     1681 gccggattgg gctgccaccc gccgaattct gaaattcaaa gtatcggcaa tcaacaaatt
     1741 acctgccgcg tcagttctca cagccgaggg caagttcaga agagcactgg ttgcggggcc
     1801 tccgtcacca ctgaaactac cactgtcatg accaatcggg ccgcttccgg caactgtcgt
     1861 aatgattcca ttctgaacat tcacccggcg aatgcgatgc gaagtcaatt cggtaatcaa
     1921 gagatttccg gcagcatcca gatgaacacc aaccgggtca aagacttgag cctgggttgc
     1981 gggaccaccg tcaccaagcc cgttttggcc tccacccgcc acggttgtta cccgtccgtt
     2041 ggtgacacgc cagacgcgac ctccatcccc gagcgtcagg taaagatttt caccggttgg
     2101 atcaaccgcc accccaaaac aattcaagat acttgattca agcgccagat caccgtctgc
     2161 tcctccaccg aaggtatctc catctcccgc aatagattga atactgccag tggtggcatc
     2221 cactcgacgg atatagccat tcccctggtc tgaaataaac aaatttccag tttggtcaag
     2281 cgtcacggct ctaggaatgt aaagtccggc tcgatttgcc ggaccgccat ccccctgaaa
     2341 ggtctgccga ccactgcccg ccaccgtgtt gatggtgctg gtttgggcat ctacgcgccg
     2401 gacacgatca ctgccgacat ccgtcagaaa cagattgcca gtcccatcaa tcgccacgcc
     2461 agtgacatcg gcgatttccg cctgagttgc cggaccaccg tcaccgatca aacccgatcc
     2521 cccatttccg gcaaaagtat caataatcaa tgagctggca ttgatccgac ggacccggct
     2581 attaaaaaag tcagcg
//
LOCUS       DAOQDO010000764         2593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34334_length_2593_cov_1.810087, whole genome shotgun sequence.
ACCESSION   DAOQDO010000764 DAOQDO010000000
VERSION     DAOQDO010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34334_length_2593_cov_1.810087"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2461)
                     /locus_tag="PKZ53_12515"
     CDS             complement(<1..2461)
                     /locus_tag="PKZ53_12515"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNJ41308.1"
                     /translation="MSLLCPITAQPSQRHTQIFKIHQIWLLIFLVSALSLWPLTRLHA
                     YDQSSTCSQLRACSVTESTVQGSLAKLSTVPRTAGDNVFYFLDQFSVGSQGVQLDLGE
                     GFPSAQVKQVFIVVTGPEASQIEGAVNVKNAFPEANLWIINPNGLIWEKNATIEAGGS
                     VVCSTAPSVEFANGHILKARYPQAQPGEPVGLRLNGERQATIRVAGTLQVAAGKWLAL
                     AANRVEQVGHLVSQGGYISVVALAGSARIGVAGGSRFQVPQISSCSTATGLDGMAELS
                     GTLDVSNPTPGGVGGQVQILSPITEILGTIQASGFSGGGEVNLHGHKLMWAGDIQADA
                     TDGNGGTLSIDLTGRGIQTDPASATVRGGQFGNGGKIVLQSRTEVDSQPHGFFLSGDL
                     VASAGQAGKTGGSITVLGNQLDFIDARLEANGPTGGGSIQIGRLINDVQSAHQDLNVE
                     TSQNAIFTRLSASTQISANAGVNGNGGQVQVWSEQETESTASISARGGSQRGSGGQIE
                     VSSRHAVSLNGEIDVDATAGESGKLVLDPKNIVIQDTTGTFPQFNLVDPNSGAGSGFG
                     NIMTPLQNGNIVVVKTNDEFIANTNTVLYLYNGHTGALISTLRAWVTSGFPGFAEVVE
                     LNNSNFVVRCPGWGDSPVFTRGAVTWVNGTTGLSGTVNASNSLVGASSLDQVGSNRIV
                     VLKNGNYVVVSPYWDNGAIQDVGAITWGNGATGTVGPVSSSNSMIGSTPDDFEDETIA
                     QYVNPLVVPLENGNYVVDLPFWDNGAIVDAGAVTWGNGATGTVGVIGPDNSLVGGSTG
                     DRLGLYVVEGIGLYRLTALTNG"
BASE COUNT          671 a          732 c          581 g          609 t
ORIGIN      
        1 ttccattggt caaagcagtg aggcgataca acccaattcc ttccacgaca tacaacccaa
       61 gccggtcccc agtggatccg cccaccaaac tgttgtctgg cccaatgacc ccaacggtgc
      121 cagtagcccc attgccccag gtcaccgcac cagcatccac aatggccccg ttatcccaga
      181 aaggaagatc aaccacatag ttcccatttt ccaacggtac aaccaggggg ttgacatact
      241 gtgcaatcgt ttcatcttca aaatcatcag gcgtacttcc gatcatactg ttgctggaac
      301 tcactggacc aacggttccg gtggcgccat ttccccaggt gatggcacca acatcctgga
      361 ttgccccatt gtcccaatat gggctgacaa caacataatt gccgttcttt aagacaacaa
      421 ttcgattgct cccaacctga tctaaagaag aggctccaac taaactattg gaagcattca
      481 cagttccgga gagaccagtc gttccattca cccaggtgac agctccccga gtaaaaacag
      541 gactatcacc ccaccctggg caacggacca caaaattgct gttgttgagt tcaaccactt
      601 ctgcaaaacc aggaaatccg ctcgtgaccc aggctctgag ggtactgatt aaagccccgg
      661 tatgaccatt atagagatac aggaccgtat ttgtgttcgc gataaactca tcgtttgttt
      721 tcacaaccac aatgttcccg ttctggagtg gtgtcatgat attcccaaat cctgatccgg
      781 ctccagaatt tgggtcaacc agattaaatt ggggaaaagt acctgttgta tcctgaatca
      841 caatgttttt agggtcgaga accagtttcc cgctttctcc tgccgttgca tccacatcaa
      901 tctcgccatt gagcgagacc gcatgtcggc ttgaaacttc aatctgacca ccgcttccgc
      961 gttgactccc acctctggca ctgattgaag cagtgctttc ggtttcctgt tctgaccaca
     1021 cctgtacctg gccaccgttc ccattgacac cagcattggc cgaaatttga gtactggcac
     1081 ttaatctggt gaaaatggca ttctggctgg tctcaacgtt caggtcttga tgagcggatt
     1141 gaacatcatt gatcaatctt ccaatctgaa ttgagccacc accagttgga ccattggctt
     1201 ccaatcgggc atcaatgaaa tccaattggt ttcccaggac cgtgattgac cctccagtct
     1261 ttccagcttg cccggcagat gccaccagat cgcctgaaag aaagaagcca tggggttgag
     1321 aatcaacctc agtccggctt tgcagaacaa tcttgccacc atttccaaac tgaccgccac
     1381 gaacggtggc actggctggg tcagtctgga ttccacgtcc ggtcagatct atggaaagtg
     1441 tacctccatt gccatcggtg gcatcggcct ggatatcccc cgcccacatc agcttatggc
     1501 cgtgaaggtt cacttcccca ccccctgaaa acccactggc ctgaatggtt cccagaattt
     1561 cagtgatcgg tgacagaatt tgaacctgac caccaacacc accaggagtt gggtttgaaa
     1621 catccagggt tccactcaat tcagccatcc cgtccagtcc cgttgccgtt gaacagcttg
     1681 agatctgagg aacctggaat ctcgatccac cagcgactcc aattcgtgcg gaaccggcca
     1741 gggcaacgac tgagatatac ccaccttgcg aaaccaggtg accaacctgt tcaactcgat
     1801 tggccgccag cgccagccat tttccagccg caacctggag tgttccagcc actcggatcg
     1861 tcgcctgtct ttcaccattg agccgtaacc caactggctc accaggctgg gcttgtggat
     1921 agcgtgcctt caagatatga ccattggcaa attcgacgga gggagcagtt gaacatacca
     1981 ccgagccacc ggcttcaatc gttgcatttt tttcccaaat cagaccattt ggattgatga
     2041 tccacagatt agcctctgga aaggcatttt taacgtttac cgcaccttca atctgacttg
     2101 cctccggccc agtgaccacg ataaatacct gtttgacctg tgcggaggga aagccctcgc
     2161 ccagatcaag ttggactccc tgagaaccaa ccgaaaactg atccagaaaa taaaacacat
     2221 tgtctccagc ggttcttggc accgttgaaa gtttggccag tgaaccctga acggttgatt
     2281 cagtcaccga acaagccctc aactgggaac aagtcgatga ctgatcatag gcgtgcagtc
     2341 gggtgagcgg ccaaagtgac agcgcgctta ccaggaaaat caagagccaa atctggtgga
     2401 ttttaaaaat ctgagtatga cgctgactgg gttgggcagt gattgggcaa agcaggctca
     2461 tattttatcc ttagaggaaa ccggttgaaa aatcaggaaa cagaattgtg gatacgtgtt
     2521 ggccaaactc tatccaggcc aggggactta cctggaatca gatattgaaa cgattcttga
     2581 aagggaaaaa ctc
//
LOCUS       DAOQDO010000765         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34350_length_2592_cov_3.096965, whole genome shotgun sequence.
ACCESSION   DAOQDO010000765 DAOQDO010000000
VERSION     DAOQDO010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34350_length_2592_cov_3.096965"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PKZ53_12520"
     CDS             complement(<1..1044)
                     /locus_tag="PKZ53_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNJ41309.1"
                     /translation="MNSIPLYPRLRRWIATLFSVCLILGPGLSIWATEKRRSVETVDI
                     ILRGGQVVTMDAERHIYENGLVAIKGDKIVAVGDASDLGSRYRAKTLIDTTGKAVIPG
                     LINTHTHVPMSLFRGVADDLLLQDWLQNFIFPAEAKNVTAEFVRAGTRLGCLEMILGG
                     TTCFVDMYYFEDHIADETQKAGMRAVLGETVIDFPVPDAKTPQDGLKYAEAFIKNYKG
                     NPLITPAIAPHAPYTCSPDLLKAAKALSDRYEVPLVTHLAEDYSEVDIIKNRYQARPV
                     EHVEKMGLLGPRVIAAHVIQVTPEEIQLLKKHGVGVAHNPQSNMKLAAGVSPVPDMLK
                     AGLGVGLGTDGAAS"
     gene            complement(1159..1818)
                     /locus_tag="PKZ53_12525"
     CDS             complement(1159..1818)
                     /locus_tag="PKZ53_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41310.1"
                     /translation="MELLTYGILAIAGLQLCILIGMLVALMKVATIIQSLHGQTEPLI
                     AQTRTTVESLKPLLENLNATVIETKPLVEQARASVMAVTPVLEQTQQILATTNETTLL
                     AKQAVTTLKTEAEACMAAVTTTTREITRMTEEEAREIRDLVQDTADKIHLQIERFDRV
                     ASRTALRIDDTAGLVQRDVLKPISEITAVLAATKGFLEVLFAEERKQIDQAYQDEEMF
                     I"
     gene            complement(1949..2353)
                     /locus_tag="PKZ53_12530"
     CDS             complement(1949..2353)
                     /locus_tag="PKZ53_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNJ41311.1"
                     /translation="MAEDSASTKLTFFLVGAGIGAVVALLFAPKSGRELRGDIADASK
                     RSVEYTRDNARRLGSKASELYETGREKATHLNEQVKERTSGLIGAGRERVEEQRQRLA
                     AAIDAGKRAYQDKKAEVQALEAALEETEPEEV"
BASE COUNT          590 a          693 c          678 g          631 t
ORIGIN      
        1 tgaagcagcc ccatctgtcc cgagcccaac cccgaggcca gcctttaaca tatctggcac
       61 gggtgagacc ccggcggcga gtttcatatt gctctgaggg ttgtgcgcca ctccgacgcc
      121 atgttttttg agaagttgga tttcttccgg ggtgacctgg atcacgtggg cggcaatcac
      181 gcggggcccg agcaggccca ttttctcaac gtgttcgacc ggacgagcct gatagcggtt
      241 tttgatgata tcaacttcgc tatagtcttc ggccaggtgg gtcacgagcg gtacttcata
      301 gcggtcagac aatgcttttg cggctttgag taaatccggt gaacaggtat agggcgcgtg
      361 cggcgcaatg gctggtgtaa tcagtgggtt ccctttgtag ttcttaatga aggcttcggc
      421 gtatttcagg ccgtcctgcg gcgttttggc gtctggcact ggaaaatcaa tcaccgtttc
      481 gcccaaaaca gcccgcatcc cggccttttg ggtttcgtcc gcaatgtggt cttcaaagta
      541 gtacatatcc acaaagcagg tggtaccgcc cagaatcatt tccagacagc caagccgggt
      601 tccggcacga acaaattccg ccgtcacatt tttggcttcc gctgggaaaa taaaattctg
      661 caaccagtcc tggagcaaca agtcatccgc cacgcctcgg aaaagagaca tcggcacgtg
      721 ggtatgggtg ttgatcagac ccggaatcac agctttgcca gtggtatcaa tcagcgtttt
      781 ggcccgatag cgcgacccaa gatcgctggc atcacccacc gccacgattt tatcgccctt
      841 gatggcaacc agtccgttct cataaatgtg gcgttcagcg tccatggtta ccacttggcc
      901 gccacgcaaa atgatgtcaa ccgtttcgac cgaccgcctt ttctcggttg cccaaatgct
      961 caaacctgga ccgaggatca agcagacgct gaacaaggtc gcgatccagc gacgcagccg
     1021 aggatacaaa ggaattgagt tcatagattt gctcactctg caattcaatt tttcaagaaa
     1081 cagacacgaa agcggtcagc ctcaacacac tcaggctgac cgcttcgctg gatgagggtt
     1141 cgaccattcg gtacgtgctc agataaacat ctcttcatcc tggtaggcct ggtcaatttg
     1201 tttgcgttct tcggcaaaca gaacctccag aaagcctttg gttgcggcca gaacggctgt
     1261 gatttcgctg attggcttga gcacatcgcg ctggaccagg ccggctgtat cgtcaatgcg
     1321 caatgctgtc cgcgaagcaa cccggtcaaa acgttcaatc tgaagatgaa tcttgtcagc
     1381 cgtatcctga accagatcac ggatttcacg ggcttcttct tcagtcatcc gggtgatttc
     1441 gcgtgtggtt gtggtgactg ccgccatgca ggcttcagct tcggttttga gcgtggtgac
     1501 agcctgtttg gccaggagtg tggtttcatt ggtggtggcc agtatctgct gggtttgttc
     1561 cagaaccgga gtcaccgcca tcaccgaagc ccgcgcctgc tcaaccaggg gtttcgtttc
     1621 aatgaccgtt gcatttaagt tttcgagcaa tggtttgaga ctctcaaccg tcgtgcgtgt
     1681 ttgggcgatg agtggctcag tctggccatg taaactctgg ataatggtgg ccactttcat
     1741 caaggcaacc aacataccaa ttaagatgca gagctgaagg ccagcaatcg ccagaattcc
     1801 atatgtcaag agttccatat caatgacacc cgtttactca atcttaaacc accgtgagta
     1861 cgtgaagaaa aaaggaggcc caggagccct ggctccaatg cctcctcttg gctaagttgt
     1921 caaacacaac gtgatgaaaa cgagagggtt atacttcttc tggttcggtt tcttcgaggg
     1981 cggcttcgag ggcctgaact tcggcttttt tgtcctgata cgcccgtttg ccggcatcaa
     2041 ttgccgctgc cagtcgctgc cgctgttctt cgacccgttc gcgtccggca ccaattaagc
     2101 cggaagttcg ttctttgacc tgttcattga ggtgagtggc tttctcacgc ccagtttcat
     2161 acaactcaga tgccttgcta ccaagccgac gggcattgtc ccgggtatat tcgacgctac
     2221 gcttgctggc atcagcaatg tcgccgcgga gttcacggcc tgattttggc gcaaacaaca
     2281 gcgcaacgac cgcgccgatt ccggcaccga ccagaaaaaa ggtaagtttt gttgaggctg
     2341 aatcttcggc catgggtaaa catcctccta aaaaatatgc ttggttcgat caaaaatggg
     2401 tgctgaaacg aaaagacaag ggattgcact tgcggactct accacaagaa ccctgaagca
     2461 aaatagcgaa atagggaagc cggtagtggg tcgcacggag cgagtcgttg aaaacacacc
     2521 gcttgccagg tgatttgccc cccattgaaa cccgatccgc gccatatgta gacgggatta
     2581 aacttcgtcg tc
//
LOCUS       DAOQDO010000766         2589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34446_length_2589_cov_2.731255, whole genome shotgun sequence.
ACCESSION   DAOQDO010000766 DAOQDO010000000
VERSION     DAOQDO010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34446_length_2589_cov_2.731255"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            517..>2589
                     /locus_tag="PKZ53_12535"
     CDS             517..>2589
                     /locus_tag="PKZ53_12535"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41312.1"
                     /translation="MRILFCGLFGLILILTCGTTPVFGLDPAKAITQYNQRVWQDGLP
                     HTSVTTIVQTRDGYIWLGTYEGLVRFNGFDFTAFDSRNTPAFSVNGILTLVEGQDGSL
                     WIGTNGGGVVRYSQGRFSTYSTLQGLSNNYIFCTTLDKSGNLWVGTDGGGVNCIQANG
                     TITKITSKDGLSSDYVRSLTVDSKGVLWVGTTNGGLNRIHNGTITMYTTREGLADNSL
                     LSLFETRDGVLWIGTGRGGLCSFKDDKLETLNVTSGVLSDPILSFLEDRDGNFWIGSG
                     GSGLLRFSNGKLSVFTMKEDLPNNVVRALWEDREGNLWAGTDGGLVRFKDGKLTTFGT
                     KEGLSSNDTRAIFEDSQHRIWVGTIGGGLNCYEKGVWRAYTTQDGLGRNIVRALCEDK
                     DGNLWVGTDVGGLCRFRDGKFTVYTTANGLNHNTIRALCVDHEGTLWVGTYGGGINRY
                     RDGVFTSVTTENGLARNVIFAIYEAHDHTLWISTFGGGVTCVKDGKYSTLTTKDGLAS
                     DQVWSFHEDADGVMWIATNAGISRYRDGKFTSINSKDGLINDAVFQILEDNLGRLWGS
                     GSRGVFSINRQALADYLDKKISQLPILAYGKSDGMASSQCTAAAYPAGFKAHDGRLWF
                     PTTGGVSMVNPASIKLNETPPPVIIEKFIVDLQPVERADSLVLSPGKNKFEFHFAGLS
                     FAAPEKVRF"
BASE COUNT          631 a          578 c          708 g          672 t
ORIGIN      
        1 actgggaaag ggtaatacca ttgagggttt agggttcagg gttcagggtt ttcaccgcgt
       61 agcggtggtt gaattgaggc aggtcgttta cggcctgtaa ataaagacat aaatttcgaa
      121 aaccccgaac ccggaacccg gaactacatt tttttgcgtt ttttctggaa atttctttaa
      181 cgaactccca ccttctgagt agaatggctc ccgccacctt actcgcaact ttgattgccg
      241 aacacattct gaaattgatt ttgtctgcca acagtgactt ctctggtgga aagaactttc
      301 ctgtggttgc tggtgaaaaa accttgcctc tcagttgaga cctgcggtct ggtcgtgccc
      361 agttgttggt tccaatgttt gtggaaagat agaaaattaa ggtttttcga gtcacaaatg
      421 cagcctgaac gaaagtcacg gtctacctga gtcaaaacct tcagctagac caaatttgcg
      481 atcacgcacc accattgtca cggcaacacg gtcaaaatgc gcattttatt ttgcggactc
      541 tttggactga tcctcatact gacgtgcgga acgacaccag tttttggatt agatccagcc
      601 aaagccatca ctcaatacaa ccagcgggtc tggcaggacg ggctgcctca tacttcagtt
      661 accaccattg tccagacacg ggacggatac atctggttgg ggacgtatga agggttggtt
      721 cggtttaacg ggtttgattt tactgcgttt gacagccgca acacaccagc tttttcagtg
      781 aatggcattc tgacgctggt ggaaggtcag gatggctcgc tgtggattgg aaccaacggt
      841 ggcggggttg tgagatatag tcaggggcga ttttcaacct actcgacgtt gcaagggctt
      901 tcgaataact acattttttg taccacgctc gataaatcag gaaatttgtg ggttggaacc
      961 gatggcggcg gcgtgaattg cattcaagcc aatgggacga ttaccaaaat cacgtccaaa
     1021 gatgggcttt caagtgatta tgtccggtcg ctcacggttg attccaaagg ggttttatgg
     1081 gtcggaacca ccaacggcgg gctcaatcgg atccacaatg gcacaatcac gatgtatacc
     1141 acccgcgaag ggctggccga taattcgctt ttatctttgt ttgaaactcg tgacggggtg
     1201 ctgtggattg gtaccggtcg aggtggcctg tgctcattca aagatgacaa actcgaaacg
     1261 ctcaatgtga ccagcggtgt tttgagcgat ccaatccttt cctttttaga agatcgggac
     1321 ggcaatttct ggattgggag cgggggcagc gggctcttac ggtttagcaa cggtaaactc
     1381 tcggttttca ccatgaaaga agacctgccc aacaatgtcg ttcgggcatt atgggaagat
     1441 cgcgaaggca atctctgggc tggaaccgat ggcgggctgg tgagatttaa ggatggaaag
     1501 ctcaccacct ttggtaccaa agaaggttta tcaagcaacg atacccgggc catttttgaa
     1561 gacagccagc accgaatatg ggttggcacg attggcggtg ggctcaattg ttatgaaaaa
     1621 ggcgtctggc gggcctacac cactcaggat ggcctgggcc gcaatattgt ccgggcactt
     1681 tgtgaagaca aagacggcaa tctctgggtt ggaactgatg ttggcgggtt atgtcgtttt
     1741 cgtgatggca aatttacggt gtacaccacg gccaacgggc tcaaccacaa tacgatccgc
     1801 gccttgtgcg ttgatcacga gggaaccctc tgggttggca cgtatggcgg cgggatcaat
     1861 cggtatcggg atggtgtgtt tacttcggtc acgactgaaa atgggctggc ccgcaatgtc
     1921 atttttgcaa tctatgaggc acacgatcat acgctttgga tcagtacctt tggcggcggc
     1981 gtgacctgtg tgaaagatgg gaaatattcg accctgacca ccaaagatgg gctggcgagc
     2041 gaccaggtat ggtcatttca tgaagacgct gatggtgtga tgtggattgc aacgaatgct
     2101 ggtatctcac gctatcggga tggaaagttt accagtatca attcaaaaga tgggttgatc
     2161 aatgatgctg ttttccaaat tctggaagac aatctggggc ggctgtgggg aagtggcagc
     2221 cgcggagtgt tttctatcaa ccgtcaggcc cttgcggact atttggacaa aaaaatcagt
     2281 cagttaccta ttcttgccta tggaaaatcg gatggaatgg ccagcagtca atgtaccgcg
     2341 gcggcttatc ccgccgggtt taaagcgcac gatggccggt tgtggtttcc aaccactggc
     2401 ggtgtcagca tggtcaatcc agccagcatc aagctcaatg aaaccccgcc tcccgtcatt
     2461 attgaaaaat ttattgttga tttgcagcca gttgagcggg ctgactcact ggtgttaagc
     2521 cctggaaaaa acaagtttga attccatttt gccggattga gttttgccgc acctgaaaaa
     2581 gtccggttt
//
LOCUS       DAOQDO010000767         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34533_length_2585_cov_3.371542, whole genome shotgun sequence.
ACCESSION   DAOQDO010000767 DAOQDO010000000
VERSION     DAOQDO010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34533_length_2585_cov_3.371542"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1509
                     /gene="folP"
                     /locus_tag="PKZ53_12540"
     CDS             160..1509
                     /gene="folP"
                     /locus_tag="PKZ53_12540"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNJ41313.1"
                     /translation="MPTNQLKALRVPQSVQIFVVRQTAPDQSIPNFEVLLLRRIQSRG
                     HFWQPITGRVEPGETITETARRELFEETGLTPQDGILFDAGYEHSYPLQEEWLVNYPA
                     GTTHNDEFAFGFLASSSAPIHIDPSEHESAIWVPFEEALTYLIWHGNQVALRHVQTRL
                     TPYRRPFQWHLPNHTLQLGHRTLLMGIVNITPDSFSDGGKYYEPSRAIEHALQLTQDG
                     ADIIDLGAESTRPGADPIGAEEELRRILPVLEGLRPKLKIPISVDTYKASVAEKVLTA
                     GADIINDITGLNYDPAICHIISKTRAGVVLMHSRGTPGQLHTLPPVANILENVRTGLT
                     SSIAQARQAGISAQSIVLDPGIGFGKSGNDNLLLHAHLNYFAELGFPLLVGTSQKSFL
                     GTILNQQTPSERGWGSAASVTLAIQQGVHLVRVHDVHQMRQCCLVADAILSRAHLFL"
     gene            1777..>2585
                     /locus_tag="PKZ53_12545"
     CDS             1777..>2585
                     /locus_tag="PKZ53_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ41314.1"
                     /translation="MTHSRRYAWYVLLILTLTQFVNYVDRQVVAPLLPLIQQDLHLSD
                     SQLGLLGTGFMIVHSLMAIPLGVLADRWERRKIVSMGLGFWSVATVFSGLANSFWQLM
                     GARAAIGIGEAAYAPAATSMLNDLFPKAEWGKVIGIFNAGLFVGGACGLVVGGILGKY
                     LGWQACFFVVGLPGLALTVVNWLVKEPARGQEYKEKHHFSTVLSIPSLWVVILAAACV
                     TFSAGALVHWLPTFLSRYHGFDVGKGAITLGAVGISAGLLGVVSGGVIADWL"
BASE COUNT          577 a          727 c          607 g          674 t
ORIGIN      
        1 ccagggacat cgtcatcaag aatccacccg gcctgaaggg cgatgccgcc gaccccaatt
       61 ttcagatcgc caagacccgc taacccccgg attgcaaccg tctacctcac gcttaccaga
      121 aacgaccctg tctatccttc cttcgaagca acttcttcaa tgccaaccaa tcagctcaaa
      181 gcccttcggg tccctcaatc cgttcaaata tttgtcgtcc gtcagacggc ccccgatcaa
      241 tccatcccaa attttgaagt tcttctgctc cgacggattc aatcccgggg gcatttctgg
      301 cagcccatca ctgggcgcgt tgaaccagga gaaaccatta cggaaactgc ccgtcgggaa
      361 ctgtttgaag aaaccggcct taccccacag gatggaattt tatttgatgc aggatatgaa
      421 catagttacc cgctccagga agaatggctt gtgaattacc cggctggcac aacccataat
      481 gatgaatttg catttgggtt tttggcctcc tcgtctgcgc caatccacat tgaccccagt
      541 gagcatgaat cagccatctg ggtaccattt gaggaagctc tcacgtattt gatctggcat
      601 ggaaatcaag tggcactccg ccatgttcag acccgtctta ccccctaccg ccgccctttc
      661 cagtggcacc tccccaatca cacacttcag cttggtcacc ggactcttct catggggatc
      721 gtaaatatta cacctgactc tttttctgac ggagggaaat attacgaacc gtctcgcgcc
      781 atcgaacatg cattacagct tacccaggac ggagctgaca taattgattt aggcgccgag
      841 tctacccgcc caggtgccga tccaattgga gcagaagaag agttacgccg gatcctcccc
      901 gttcttgagg gcttgcgacc aaaactcaag attccgattt cagttgatac ctataaagcg
      961 tccgtagctg aaaaagtgct gacagcgggg gcagacatta tcaacgacat tacaggactt
     1021 aattacgacc cagctatatg tcatatcatc tcaaaaacca gggccggggt tgttctgatg
     1081 catagccggg gaacgccagg ccaactccat accctccctc ccgtggcaaa tatcctggag
     1141 aacgtgcgca caggcctgac atccagtatt gcacaggccc gtcaggcagg aatttcagct
     1201 cagtcaattg ttcttgatcc cggaattgga tttggaaaat cagggaatga caacttgctc
     1261 cttcatgctc atctcaatta ttttgctgaa ctcggctttc cgcttctggt cggtacgtct
     1321 caaaaatcat ttctggggac aatcctgaat cagcagactc catctgagcg aggctggggt
     1381 tctgccgcca gcgtgaccct ggctatccag cagggagtcc atctggtccg agtgcacgac
     1441 gtacaccaaa tgcgacaatg ttgcctggta gccgacgcca tcctttcccg ggcccacctc
     1501 tttttatgat ttccgctctg catcaaagtt tcaacttcag gacacaacgg atactgaatc
     1561 tgtcgtttct caatccgcat ttaccttcct gaggtaccaa gcaaccttgc accagcctga
     1621 atgatcaacc tacaatgcga caacattgac cttgcatccg agatttcacc tggtactgac
     1681 ggcttcctgt catcccttca ctttttggga attcggtgac tgccctccct ggccctgcaa
     1741 gaacaatcta tcccagcctc tgtaatatat tgaactatga ctcacagtcg ccgctatgcc
     1801 tggtatgtcc ttctcatttt gactctgacc caatttgtca actatgtaga ccgccaggtt
     1861 gttgccccct tactcccctt aattcagcaa gacctgcacc tttcagatag tcaacttgga
     1921 ctgcttggta ctgggtttat gatcgtccac tcattgatgg cgattccact cggagttctg
     1981 gctgatcgct gggaacgccg gaagatcgta tccatgggcc tgggcttctg gagcgtggcg
     2041 actgtcttct ctggactggc aaattccttc tggcaactca tgggtgcccg ggccgccatt
     2101 ggaattggcg aagccgctta tgctccagcg gcaacctcaa tgttaaatga cctgttccca
     2161 aaagctgaat ggggaaaagt tatcggcatt tttaatgctg gcctttttgt tggtggcgcc
     2221 tgtgggctgg tggttggagg aattctcggc aaataccttg gctggcaagc ctgttttttc
     2281 gtggttggct tacccggact ggcattgact gtcgtaaact ggctcgtcaa agagcctgcc
     2341 cgtggtcaag agtataagga aaagcaccat ttttcgacgg tgctctcaat cccttcattg
     2401 tgggttgtca ttttagcggc ggcctgcgtt accttttcgg ctggggctct ggttcactgg
     2461 cttccaacat ttctttcccg gtaccatgga tttgatgttg gaaaaggggc cattaccctg
     2521 ggagccgttg ggattagcgc tggattgctg ggggttgttt ccggaggcgt cattgctgac
     2581 tggct
//
LOCUS       DAOQDO010000768         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34588_length_2583_cov_3.653085, whole genome shotgun sequence.
ACCESSION   DAOQDO010000768 DAOQDO010000000
VERSION     DAOQDO010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34588_length_2583_cov_3.653085"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..603)
                     /locus_tag="PKZ53_12550"
     CDS             complement(64..603)
                     /locus_tag="PKZ53_12550"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HNJ41315.1"
                     /translation="MKRYWKIIVFSLIAIGVVGIIVVAGAAYYVTGTPVYSLYVLRQA
                     LREQDRDQFYHHFDVRRVIEHTLEQKVSPKIKSYLPQMGRETALDLAESIVRRKIDER
                     LADPESISFGKMTLASINQQGQTAQVTLRNPKDGSTTVVGLEQMSDRHWKIVEIDLSQ
                     ADASVSPEELLQGIRKNEE"
     gene            complement(600..1811)
                     /locus_tag="PKZ53_12555"
     CDS             complement(600..1811)
                     /locus_tag="PKZ53_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNJ41316.1"
                     /translation="MTPKIYLDNAATTAVAPEVVEAMLPYLTQAYGNASSVHSFGQQA
                     RAAIERARRQVAMLIGAAESSELIFVSGGTEANNLAIQGIAKSYGRPGQHIITTAFEH
                     PAVLGPCAALENRGFRVTRLPVYETGLIRLEDVRAALTDDTILMTIMMANNEVGTLQP
                     VAEIGKLVQEVREHRPHLYFHVDAVQAAGKVPINVKELGVDLLSISGHKIHAPKGVGA
                     LYLRKGVRIAAQQLGGHHERDRRAGTENVAGIVAFGKAVELASRQIAEITEVARLRDH
                     LEQSLIETIPGLQVNGDPNSRLPNLTNLSFDTIDAESLLIALDLKGIAVSTGAACSSG
                     STEPSHVLTAMGFSRERVRSSLRLSLSRFTTPAEIDRVIEVLPELVKRQRQKTSGANL
                     ECGGLTPLSSR"
     gene            2053..>2583
                     /locus_tag="PKZ53_12560"
     CDS             2053..>2583
                     /locus_tag="PKZ53_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HNJ41317.1"
                     /translation="MLGKRLVWLALLFLAGTIGVRAEEGRLLRYPDVHQDQVVFVYSG
                     DLWIASTQGGPARRLTTDEGQELFPKFSPDGKYIAFTGEYDGNRDVYVMPATGGEPKR
                     LTYSLDMDALPDRMGPDNLVLGWTPDSKRVLFRSRRASLNAWTGQLYTVSLEGGLPEQ
                     LPVPRGGFTSYSPDGSK"
BASE COUNT          615 a          706 c          633 g          629 t
ORIGIN      
        1 accaagaacc aagaaccaag aaccaagaac caagaaccaa gaaccaagaa ctaagccaat
       61 tcttcattct tcattttttc ttattccttg cagcagttct tcgggcgaga cgctggcatc
      121 agcctgggaa aggtcaattt caacaatttt ccaatgtcgg tcactcattt gctccaggcc
      181 cacaaccgta gttgaaccat cctttggatt gcgcaacgtg acctgagccg tttggccctg
      241 ctggttgatc gaagccagcg tcatctttcc aaaggaaatt gattcagggt ctgccagtcg
      301 ttcgtcaatt tttcggcgga caatggattc agccagatcc aaagcggttt cccttcccat
      361 ctgaggcagg tagcttttta tcttgggact cactttttgt tccagggtat gttcaatgac
      421 ccggcggacg tcaaaatgat gataaaactg atcacgatcc tgttcgcgca gtgcctgacg
      481 aagcacatag agcgagtaca ctggtgtccc ggtcacataa taggcagccc cagccacaac
      541 gatgataccg accacaccaa tggctatcag gctgaaaaca atgatcttcc agtatctctt
      601 catcgtgatg aaagcggcgt caagccgccg cactccaaat ttgcgccgct ggtcttctgg
      661 cgctgacgtt tgacaagttc tggtaaaacc tcaatcacac ggtcaatttc tgccggtgtg
      721 gtaaagcggc tcaggctcaa ccggaggctg ctccgaactc gctccctgga aaaccccatc
      781 gcggtcaaca cgtggctcgg ctcggttgaa cctgacgaac aggcagcccc ggttgaaacc
      841 gcaatccctt ttaaatcaag cgcaatcaac aaactttcag catcaatcgt gtcaaaactc
      901 agattggtca aatttggcag gcgtgaattg ggatcaccat tgacctgaag cccagggatt
      961 gtttcgatca gagattgttc cagatgatcc cgaaggcggg caacctcagt tatttcagct
     1021 atttgccgcg aagccagttc cacggctttc ccaaacgcga cgattccagc cacgttttca
     1081 gttccggccc ggcgatcccg ttcgtggtgg ccaccgagtt gttgggccgc aatccgcaca
     1141 ccttttcgca ggtatagcgc gccaacgcct ttcggcgcat ggattttatg acccgagatg
     1201 gagagcaaat ccacgccaag ttcttttaca ttgatcggca ctttccccgc cgcctgaacc
     1261 gcatccacat ggaaatacag gtgggggcgg tgttcccgca cttcctgaac cagctttcca
     1321 atctctgcaa ccggttgcag agttccaact tcattattgg ccatcatgat cgtcatcaaa
     1381 atagtgtcat cagtgagtgc tgcccgaaca tcttcgagcc gaatcagtcc agtttcatat
     1441 actggaagcc gggtcacccg aaacccacgg ttttccaggg cggcacaggg gccaagcacg
     1501 gcaggatgtt caaaagcggt ggtgatgata tgctgtccgg gtcggccata acttttggca
     1561 atcccctgaa tcgccaggtt gttggcttca gtgccgccgc tgacaaaaat gagttcagag
     1621 ctttcagccg caccgatcag catcgcgacc tgccgccgcg cccgttcaat cgctgcacgg
     1681 gcttgctgac caaatgaatg cactgatgat gcgttcccat acgcctgggt caggtacggc
     1741 agcattgctt caacaacttc aggggccacg gcagtcgtgg ccgcattatc aagataaatt
     1801 tttggggtca taggctgaga agagtgagtc gcgaagagtt caaccacaca atagtgacta
     1861 gctttcttcg gataaagcaa atcccttcgg cgtatctcac gctaaagaat aaaactttct
     1921 tcgattgcat atttcaagaa tgctctggtg ggtgtaaaag gtagtaactg actacttgct
     1981 actcgctttc tcacaattga taactgccgc actcaacaac aatagaattt tatttcctgg
     2041 aggtttaact tcatgcttgg caaacgcctg gtttggcttg cgctcttgtt tttagccggc
     2101 acgattggtg ttcgtgctga ggaagggcgg ctgcttcgat atcctgatgt gcatcaggat
     2161 caggtcgtct ttgtgtattc cggcgatttg tggatcgcct caacccaggg cggtcctgcc
     2221 cgccgactga ccacagatga aggtcaggaa cttttcccga aattctctcc tgatggaaaa
     2281 tacattgcct ttaccggtga atatgacggc aatcgcgatg tttatgttat gccagccaca
     2341 ggtggcgaac ccaaacggct gacctacagt ctcgatatgg atgccctgcc agatcgcatg
     2401 ggaccagata atctggtgtt gggatggacg ccggacagca agcgcgtctt gtttcggtca
     2461 cgccgcgcca gcctcaatgc ctggaccggg caactctaca cggtcagcct cgaaggcggc
     2521 ttgccagaac aacttccggt gccacgtggc ggctttacgt cttattcacc agatggctca
     2581 aaa
//
LOCUS       DAOQDO010000769         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34643_length_2581_cov_3.041964, whole genome shotgun sequence.
ACCESSION   DAOQDO010000769 DAOQDO010000000
VERSION     DAOQDO010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34643_length_2581_cov_3.041964"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..>2581)
                     /locus_tag="PKZ53_12565"
     CDS             complement(161..>2581)
                     /locus_tag="PKZ53_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41318.1"
                     /translation="DQLLPTALSELNDWKAQTGPVLPLGDITLDTIPELARDFPHTWR
                     KLEALVPEGMHQRLKIGMHRLVVEASRAVSRLARLVGPAYFHKVSAPQPAPAVEFPPR
                     YNFERAFKQIIPHNYAWRFCRDLNQELVTIHAYLAGLEPADVLQPRLPQGYNGTGIGW
                     IDLIGNLGMVGFLVATAYQSPEEKVDYYQYRETRLKFLEAWSDTIFASEPDTIRVLYG
                     RITVPEATAAEWQKLDQATLIEHEGNRYFVRTYHEYYIEHSGISYTAIEYAPDGNFKE
                     LPGTSVVNARRNGGWLGRDNIRKLVNLVKTRGPVAWDSKIADVLSRETGLSWNAAAVL
                     WLGFPGFEGHDESRFFPASLREQLGMKVLDVNSARIELEKMCPVPIRLPLWSDVLPDE
                     IETLWTPLGTGETDPHSVVQRLIAAWNRHVGKQIEIPAELVATITKEKYYTDIKAVEL
                     LTMLANPAQSEWLHDDQEWRLFFPSTWLTYSYPVGCDWAKYNNSKFFRREVVTSWSIL
                     LPYLFVTLPVGDPLRSNLPAVMHLIWERLRNPNFFLSLDYKYFHASHGALQPQVDAFF
                     DQFGDVPYVAPQTPANEQHHGIDAGDSVALLRKQDDRSAEVEVFVRTGRIPNFERLEA
                     IAAMVKMSPRLESLFPFTQGCRDLIARIEQTPVPAGQYECNPVYSAPHVVSEIAEAFR
                     LSLDAATLCLQTLALIEPSTRNICLWNGWNSTRYKKAASELVAAEIVIEAKRERAGRN
                     IFLPGVWISPKPPDEPIEQWKLELYGAKSYGPGSYWKPLKRLLPLRPIHEIFELAWTR
                     IKERDHPG"
BASE COUNT          584 a          663 c          699 g          635 t
ORIGIN      
        1 ggtcaagctg aaacgagccc tcagtgcata cagactcgac gctatgatga cttcaagcgg
       61 aagtaactta tttcccggtc tgattttcgc ggggcattat agtttgatct ttttcggata
      121 tttcggggtt caaaatgtct ggaaattttc ggtaaggtac ttaaccgggg tgatctcgtt
      181 ccttgatccg cgtccaggcc agttcgaaaa tctcgtggat tggtcgcaac ggaagcaagc
      241 gcttgagcgg cttccagtat gagcccggcc catacgattt ggcgccgtac agctccagtt
      301 tccactgctc gattggttcg tcaggtggtt tgggggaaat ccagacgccg ggcaggaaaa
      361 tattccgccc ggcgcgttcg cgtttggctt cgatgacgat ttctgctgca accagttcgc
      421 tggcagcttt cttgtatctg gtgctattcc agccattcca tagacagatg ttgcgggttg
      481 acggctcaat caatgccagc gtttgcaagc acagcgttgc cgcgtccaga ctcaatcgaa
      541 aagcttcggc aatttctgaa acaacgtgcg gtgctgaata caccgggttg cattcgtatt
      601 gacccgccgg aactggtgtc tgttcaatcc gggcaatcag atcacggcaa ccctgggtga
      661 atggaaacag agactccaga cgaggtgaca tttttaccat ggcggcgatt gcttcgagtc
      721 gctcaaaatt tgggatacgg cctgtccgaa caaacacctc gacttcggct gatctgtcat
      781 cctgctttct cagcagcgcg accgagtcgc cagcatcaat tccatgatgt tgttcattcg
      841 ccggggtttg tggtgcgacg tagggaacat ctccgaactg gtcgaaaaac gcgtctacct
      901 ggggctggag cgctccgtgt gacgcatgga aatatttata gtcgagcgac aggaaaaaat
      961 tcggatttcg gagccgttcc caaatcaggt gcatcactgc cggcaggttt gaccggagtg
     1021 ggtctcccac gggcagtgtt acaaacaaat acggcagcaa gattgaccac gacgtaacca
     1081 cttcgcgcct gaaaaacttt gaattgttat attttgccca gtcacagcca accggataac
     1141 tataggtcag ccaggtgctt gggaaaaaca accgccattc ctggtcgtcg tgcaaccatt
     1201 cggattgggc tggattggcc agcatggtca ggagttcgac tgccttgatg tcggtgtaat
     1261 acttttcttt tgtaatggtc gccaccagtt cagccgggat ttcgatttgt ttgccgacat
     1321 gccgattcca ggcggcaatc aggcgctgaa ccaccgaatg tggatctgtt tcgccagttc
     1381 cgagcggcgt ccacagcgtt tcaatctcat cgggcagtac gtcagaccac aacggcagcc
     1441 ggatgggcac cgggcacatt ttttccagtt caattcgtgc tgaattgaca tcaaggactt
     1501 tcattccaag ctgttcacga agcgatgctg gaaaaaagcg tgattcgtcg tgcccttcaa
     1561 agcctgggaa ccccagccag agtacagcgg cggcattcca gctcaagccc gtctcacgtg
     1621 aaaggacgtc ggcaattttt gaatcccagg caaccgggcc acgggttttg accagattga
     1681 ccagctttcg gatattgtcg cggccaagcc agccaccatt gcgccgggcg ttcacaaccg
     1741 aagttcccgg caattcttta aagtttccat cgggggcata ttcaatcgcg gtgtaggaaa
     1801 tcccggaatg ttcaatgtaa tactcatgat aggttctgac gaaatagcgg ttgccttcgt
     1861 gttcaatcag cgtggcctga tccagttttt gccactcggc tgccgttgct tctggaaccg
     1921 tgatgcggcc atacagaacg cggattgtgt caggttcact ggcaaaaatg gtatctgacc
     1981 aggcttcaag gaattttagt cgggtttcgc gatactggta atagtcaacc ttttcttcag
     2041 gggattgata ggctgtcgcg accaaaaagc ctaccatccc caggtttcca atcaggtcaa
     2101 tccagccaat tccggttccg ttatatcctt gtggaagcct gggttggagc acatcagccg
     2161 gttccagccc cgccaggtag gcgtgaatcg tcaccagttc ctgattgaga tcgcggcaaa
     2221 atcgccaggc atagttgtgt ggaatgattt gcttaaacgc ccgttcaaaa ttatatcgtg
     2281 gaggaaactc aacggcgggt gccggttgcg gtgctgagac cttgtgaaaa tacgccggac
     2341 caaccagccg ggcgagccgg gaaactgcgc gtgacgcctc aaccaccagc cggtgcattc
     2401 caattttcaa ccgctggtgc attccctcgg ggaccagtgc ttcaagtttt cgccacgtgt
     2461 gcgggaaatc tctggcaagt tcgggaatag tgtccaaagt gatgtcgccg agtggaagaa
     2521 ccggtccagt ctgggctttc cagtcgttga gttcagaaag ggcggttggc aaaagctgat
     2581 c
//
LOCUS       DAOQDO010000770         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34652_length_2581_cov_2.403009, whole genome shotgun sequence.
ACCESSION   DAOQDO010000770 DAOQDO010000000
VERSION     DAOQDO010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34652_length_2581_cov_2.403009"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKZ53_12570"
     CDS             <1..970
                     /locus_tag="PKZ53_12570"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNJ41319.1"
                     /translation="PPQAPENLINLSRVGKFLNTALNPLGGIQPGIAYLGPQSQFQCG
                     MLRNMPVRIVNDVLDLDRDNNRTEEAFPIRGNRDLSASEVETILAQAIAQAYRTRGAI
                     RQPIGSFAEVNVTVVGVDGEILGIRATPDAPIFGLDVSAQKARSSCLFSRSDAAQLLR
                     RAGAGNYVDALANEGIRLDGSVAFSDRGIGFLHRPFFPDGITGSENAPLSSSIKNFSP
                     FNVGLQLDILAPSIIDILNVKAPRPLLPNIRNGLQIFAGGLPLYKGGKLVGGIGISGD
                     GIDQDDIICANGAKGFEAPADMRCDRVFVRGVRLPYQKFPRHPELP"
     gene            complement(1047..1862)
                     /gene="truA"
                     /locus_tag="PKZ53_12575"
     CDS             complement(1047..1862)
                     /gene="truA"
                     /locus_tag="PKZ53_12575"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNJ41320.1"
                     /translation="MANLNFTYRLVIEYDGTRYHGWQEQANARTVAGELRGVACEVCN
                     EKVDFGGAGRTDAGVHALGQVAHLRTSKRHNPVELQRSINNLLPADINLVRVEIAPPE
                     FHARHDALARYYLYQLATQRTAFGKKYVWWIKDHLDLRQMRKAAEHFVGLHDFAHFSQ
                     PDPSVMGNPRKSESTRVDIHRLTIDTGRGMVYVRIGASHFLWKMVRRIVGTLVVVGQG
                     QVSPDAIPELMRRGSPEIAAHTAPASGLFLERIEYPGDPKSNPTAPKLNIPSL"
     gene            2281..>2581
                     /locus_tag="PKZ53_12580"
     CDS             2281..>2581
                     /locus_tag="PKZ53_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNJ41321.1"
                     /translation="MTESRPLTILCLASYEKGQEFMRECKRQGCRTFLVTSESIADAD
                     WPRESLDDIFLIPDDNKKWKRDDVIKGISYLARTQTIDRIVALDDFDVETAAALRE"
BASE COUNT          606 a          677 c          657 g          641 t
ORIGIN      
        1 gccaccccag gcgcctgaga atctgatcaa tctcagtcga gttggtaaat ttctcaacac
       61 ggccttaaac cccttgggcg ggatccagcc tggaattgca tacttaggtc cacaaagtca
      121 gtttcaatgc ggaatgctcc ggaacatgcc tgttcgaatt gtcaatgatg tccttgatct
      181 tgatcgggac aataaccgga ctgaagaagc ctttccaatc cgtggaaacc gtgacctctc
      241 agccagcgaa gtcgaaacca tcctggccca ggcgattgcg caggcgtatc gcacccgtgg
      301 tgccattcgt caaccaattg gcagctttgc cgaagtgaat gtcacggtcg ttggggtgga
      361 tggtgaaatc cttggaattc gcgcgacacc tgatgcccca atttttggac ttgatgtctc
      421 ggcccaaaaa gcccggtcat cgtgtttgtt tagccgatct gatgcggccc agctcctgcg
      481 ccgggcaggc gcgggcaatt atgtggatgc ccttgccaat gaaggcatcc ggctcgatgg
      541 ctcggtcgcc tttagtgatc ggggcatcgg ctttctgcat cgcccatttt tcccggatgg
      601 cattaccggg tcggaaaatg ccccgttgag ttcatccatt aaaaacttct cgccgtttaa
      661 tgttgggctt caactcgaca ttctggcccc atccatcatt gatattttga acgtcaaagc
      721 ccctcgccca ctgcttccaa atatccgcaa tggcctccag atttttgctg gcggacttcc
      781 cctctataaa ggtgggaaat tagtcggtgg gattggcatc agcggtgatg gaattgacca
      841 ggatgacatt atttgcgcca atggcgccaa aggctttgaa gctccagccg acatgcgatg
      901 tgatcgcgtg tttgtgcgtg gagtgcgcct gccctatcag aagttcccac gtcatccgga
      961 actgccctag tttgtgacac aatgaccggg tgacaggatg acaaatctgt tgattgtcac
     1021 cctgttaccc ggccacctta tcaactttaa agcgagggaa tattgagttt cggtgcggtc
     1081 gggttcgatt tcgggtcgcc tggatattca attcgctcta aaaacaaacc tgaagccgga
     1141 gcggtgtggg ccgcgatttc cggtgaccca cgccgcatca attccggaat tgcatctgga
     1201 ctcacctgtc cctgacctac cacaaccagg gtgccaacga ttcgtcgcac catcttccac
     1261 agaaaatgcg aagccccgat tcggacatag accatacccc ggccagtgtc gattgtcagt
     1321 cgatggatgt cgacccgggt tgattctgat tttcgaggat ttcccatcac acttggatca
     1381 ggctggctaa aatgggcaaa atcgtgaagc ccgacaaaat gttcagccgc tttgcgcatt
     1441 tgtctgagat ccaggtgatc tttaatccac cacacatatt ttttcccaaa cgcggtccgc
     1501 tgggttgcca gttgatagag ataatatcga gccagggcat catggcgggc atggaattca
     1561 ggtggtgcga tttccactcg aaccagattg atatccgccg ggagcaaatt gttgattgat
     1621 cgttgtaact caaccgggtt gtggcgtttg gaagttctca ggtgagcaac ctggcccagg
     1681 gcgtgaaccc cggcgtcagt tcgtccagcg ccaccaaaat caaccttctc attgcagacc
     1741 tcacaggcaa ccccgcgtaa ctctccggcc acagttcgag cattggcctg ttcctgccat
     1801 ccgtgatagc gggtcccatc atattcaatt acaagtcggt aggtaaaatt caagtttgcc
     1861 atagtatatc cagtcagttt caggaatggg acgctcagaa ggcttgccga tcagagggca
     1921 attcagattc aggaatcaac cgagacaatc ctggggttca aagtccaaat gtggaccatc
     1981 agggaaaaac agccagtata gcggagcctg gtacagcatt ggcaaccgga gttgggcaat
     2041 tttggttctt ggcgaatagg aaatttcttg ctaacgtaag cgctcgctgc aaaaagtcat
     2101 cattttcttc ccgttgcaat cttttatcat atcagtgttg tggttcaaca accgggtatc
     2161 cactgggcaa ttccgagtgg cacacctctg acgaaccctc tcaatcagtg ccagttgcgg
     2221 gtagcaagtg gctgtcccct gttatatttc tatattattt ctcttgtgaa ggagtcgccc
     2281 atgacagaat cacgtccttt gacgattctc tgcctggcca gctatgaaaa aggacaggaa
     2341 tttatgcggg aatgcaaacg gcaggggtgc cgcacctttc tcgtcacttc agaaagcatt
     2401 gccgatgctg actggccgag ggaaagcctc gacgacatat ttctgattcc agatgacaac
     2461 aaaaaatgga aacgggatga tgtcatcaaa ggcatcagtt acctggctcg aacccaaacg
     2521 attgaccgga ttgtggccct tgatgacttt gacgttgaaa ccgccgcggc gctccgtgag
     2581 c
//
LOCUS       DAOQDO010000771         2577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34745_length_2577_cov_2.556305, whole genome shotgun sequence.
ACCESSION   DAOQDO010000771 DAOQDO010000000
VERSION     DAOQDO010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34745_length_2577_cov_2.556305"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2577
                     /locus_tag="PKZ53_12585"
     CDS             <1..>2577
                     /locus_tag="PKZ53_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41322.1"
                     /translation="LDGIVGTYDSSLFYFYDSLIALAGWYETAASEQGSLLKRVAANQ
                     RKLKGWARHAPQSYLHKHALVQAEYYRVLGQPQQAGMWYETAIDLAKKHNFPHEEALA
                     CELTARFYLGQRRMNIAFGYLMDARYGYQRWGAVAKVKHLDEQYSPLMARKIETRNTS
                     TTFIALPVFSTTSTGSASAGVLDLTSVVKASQALSSEIVLEKLLSKLMRILIENAGAQ
                     KGVLLFPQGGELIIEAECEAGKPARNVLQSLPSRTSRDLPQTVVNYVARACETVLLEN
                     APIDRRFGHDPYIQRNQVRSILCQPVLHQGKLTAVLYLENQTSIGAFTPQRVEMLNLL
                     SAQAAVSLENAMLYANLERKVTERTAELSILNIELGERNRQLEETRIEAEKQRERAED
                     ASLAKSRFLASMSHELRTPLNAILGFTQLLARKTKPNDEAYEHLSTILASGEHLLGLI
                     NDVLSISKIEAGQMFLNEGDFDLHLLLQSVENMFRLRAEAKGLEFHFDLMSEVPHLVT
                     GDEGKLRQVLLNLLSNAFKFTESGRIVVRTAWADQRAMFEVEDTGVGIAPEEMSRLFE
                     AFVQTQAGLKTQEGTGLGLVISRNFARLMGGDITVTSQVGKGTTFRVEISLPRMNTTI
                     IRSTPDLGRVVRVASGQRLFKMLVVDDSWENRTVLSQLLESVGFEVVTARNGREAITC
                     WQIVHPDVVWLELDMPELNGYETARAIRRLEQASVVNRHPSLEPPNRDQTILVALSAS
                     VFEQDREAILNAGFQEFIAKPYREAVIFGAMAQYLGVMYEYESPEITAERDSKSREST
                     KLIDWANHLMALPSALVSQLRETLVVGDTVAALQLTEDIRQMNPLLADEVERMVRSYK
                     FD"
BASE COUNT          603 a          652 c          726 g          596 t
ORIGIN      
        1 tgctagatgg aattgttggc acctatgatt catcgctgtt ttatttttat gattcactga
       61 ttgctctggc cggctggtat gaaaccgctg ccagtgaaca aggcagtctt ttgaaacggg
      121 ttgccgccaa tcagcgcaaa ttaaaaggct gggcccggca cgcgcctcag agttacctgc
      181 acaagcacgc actggtccag gccgaatact atcgggtcct gggccagccg cagcaggccg
      241 ggatgtggta tgaaacggcg attgatctgg ccaaaaaaca taatttcccg cacgaagaag
      301 ccctggcctg tgagttgaca gcccggtttt atcttggtca gcggcggatg aacattgcct
      361 ttggttacct gatggatgcc cggtatgggt atcaacggtg gggtgcggtg gccaaagtga
      421 agcacctgga tgaacagtac tcgccactga tggcgcgcaa gattgaaacc cgcaatactt
      481 cgaccacttt cattgcatta cctgtgtttt caacaacttc gaccggcagt gcctcagccg
      541 gcgtcctgga tctgacctca gtggtgaaag catcacaggc gttgtcgagc gaaattgttc
      601 ttgagaaatt gctttccaaa ctgatgcgga tcctgattga aaatgccggg gcacaaaaag
      661 gagtgctgtt gtttccgcag ggaggagaac tgatcattga ggccgaatgt gaagccggga
      721 aacccgcccg aaatgtgctc cagtctcttc catcccgtac ctcccgtgac ctgccgcaga
      781 cagtggtcaa ttatgtcgcc cgtgcctgcg aaacagtttt gttagagaac gccccaattg
      841 accggcgatt tggccacgat ccatatatcc agcgcaatca ggtgcgttca attttatgtc
      901 agcccgtgct gcatcaggga aaactgaccg cggtgctcta cctggaaaat cagacttcaa
      961 tcggtgcctt caccccgcaa cgggtggaga tgttaaattt gctttcggcc caggctgcgg
     1021 tgtcgcttga aaacgcaatg ctctatgcca atctggaacg caaagtgacc gaacgtacgg
     1081 ctgaactttc gattttgaat attgaactgg gcgagcgaaa tcgccagctt gaagaaaccc
     1141 ggattgaagc cgaaaaacaa cgcgaacgtg ccgaagatgc cagtctggcc aagagtcggt
     1201 tcctggccag catgagccac gaattacgca ccccgctcaa tgccattctt ggctttaccc
     1261 agttgctggc gcgcaagacc aaacccaatg atgaagccta tgaacacctg tcaaccattc
     1321 tggccagcgg cgaacacctg ctggggctca tcaatgacgt gctctcgatt tcaaagattg
     1381 aggccgggca gatgtttctc aacgaaggtg attttgattt acaccttttg ctccaaagtg
     1441 ttgagaacat gttccggttg cgggccgaag ccaaggggct ggagtttcac ttcgacctga
     1501 tgtctgaggt gccgcatctg gtgactggcg atgaagggaa gttgcgccag gtcctgctga
     1561 atttattgag caatgccttt aaatttactg aatcaggccg gattgtggtg cgcactgcct
     1621 gggcagacca gcgggcgatg tttgaagtcg aggatactgg cgtggggatt gcccctgagg
     1681 aaatgagccg gttgtttgag gcctttgtcc aaacccaggc aggactcaaa acccaggagg
     1741 gaactggcct ggggctggtc atcagccgga actttgcccg gttaatgggt ggcgatatta
     1801 ccgtcaccag ccaggtcggg aaaggcacga cgtttcgcgt cgaaatcagc ctgccacgta
     1861 tgaataccac gatcattcga tctacgccgg accttgggcg agtggtgcgg gtggcatcgg
     1921 ggcaacgcct gtttaaaatg ctggtggtgg atgattcgtg ggaaaaccgg actgtcttgt
     1981 cacaactcct tgaatcagtt gggtttgaag ttgttacggc tcgcaacgga cgcgaagcca
     2041 tcacctgctg gcaaattgtc catcccgatg tcgtctggct ggaactggat atgcccgaac
     2101 tgaatggata tgaaacagca cgggccattc gacggctgga acaggcatcg gttgtcaacc
     2161 ggcatccttc gctggaacca cctaaccgtg accagacgat actggttgcg ctctcggcca
     2221 gtgtgttcga gcaggaccgg gaagcgatcc tcaacgccgg atttcaggaa tttattgcca
     2281 aaccgtatcg cgaagccgtc atttttgggg cgatggcaca gtaccttggc gtcatgtatg
     2341 aatatgaatc gcccgaaatc accgccgaac gtgattcaaa aagccgtgaa tccaccaagt
     2401 tgattgattg ggccaatcat ttaatggcgc tgccgtcagc actggtgtcg cagcttcgcg
     2461 aaactctggt ggttggcgat accgtggcgg cgctccaact caccgaggat attcgccaga
     2521 tgaacccgtt gctggctgat gaagtcgagc gtatggtgag aagctataag tttgatg
//
LOCUS       DAOQDO010000772         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34772_length_2575_cov_3.744444, whole genome shotgun sequence.
ACCESSION   DAOQDO010000772 DAOQDO010000000
VERSION     DAOQDO010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34772_length_2575_cov_3.744444"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKZ53_12590"
     CDS             complement(<1..290)
                     /locus_tag="PKZ53_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41323.1"
                     /translation="MDTLALNIRIRYATLILGLLITVVVTVWVYKTTDTPQPKDIVPV
                     AAALIAMTALVYAAMSFHVNVQLNEIKLLQDKKQFSLSLISEWHKPDMAKLT"
     gene            443..1855
                     /locus_tag="PKZ53_12595"
     CDS             443..1855
                     /locus_tag="PKZ53_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ41324.1"
                     /translation="MQVPLVAPAPIVEQHAQAFRDLFENRCQFDHFQNYVTGLMVLEN
                     KSLANISRCVVESADKTNLSRFFSQAPWEAKEINDRRVEYMVEQTQGKRRGVKESCLA
                     IDDTLCEHVGSLFEYVARHYDHGENRYPLGHNLVTSHFVSGAVRFPIDVRLYRRYEEQ
                     TDWENTVRKHFPGREIPTKAKERKQLHKEVDAILLTDEAFRQRHEAFQTKIELAMELI
                     EDAIERRLPVGVLLMDSWYLAPELITKLKFHHLDWVSLLKKNRNLETASFTLRDGEGK
                     PIALAGPHIQVEELVKLIPRTAFRKETVAERDFWCFALNLRIPDLGRVRLVISYGTSD
                     LTGAYAVLVSNRSDWSAKKIIQTYLQRWPIETFYQDSKGHLGLDEYRMRTAEAFQKHW
                     CLVFVAYSFLHLDCLPASPPKRSSLARPIKTIGEVCRLQGQLLLHKLILKAHDLFQQG
                     SSPTQVFAQLFAKLQPALIC"
     gene            1933..2067
                     /locus_tag="PKZ53_12600"
     CDS             1933..2067
                     /locus_tag="PKZ53_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41325.1"
                     /translation="MQIGIKAVVGRGQLETKLVKQTTCVLLQTAFLVPPCLLYKFGIT
                     "
     gene            2218..>2575
                     /locus_tag="PKZ53_12605"
     CDS             2218..>2575
                     /locus_tag="PKZ53_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41326.1"
                     /translation="MNNLPNKPQLHRWQNAVLDDLKQYLINKTPFGSRAIGYLIFEHL
                     KSDPTWAYPERWLDALNTFGKSVIDPQTVQMIGYLDWMDQRPRFGGDDLFDATFRLVQ
                     TPEGEALDYIIRFCAEG"
BASE COUNT          663 a          602 c          653 g          657 t
ORIGIN      
        1 attgtcagtt ttgccatgtc aggtttgtgc cattcactaa tcaagcttaa tgaaaattgc
       61 tttttatctt gaagaagctt tatctcattt aattgaacat ttacgtgaaa actcatcgct
      121 gcataaacta acgcggtcat agcaattaat gccgctgcaa ctggtactat atctttgggt
      181 tgaggagtat ccgtggtttt gtatacccag actgtaacta caacggtaat gagtaacccc
      241 aatataaggg ttgcatagcg tatgcgaatg tttagtgcca aggtatccat tttaccctct
      301 ccttcgggaa aatattaaga atgttacccc atacggttag atttaaaggt ctcgagtgtt
      361 gtgttttaga tttacccccg accaggagta gggtaaaaag gagtgaaaag cgaaaaagaa
      421 cgaaaagaaa ggggtttttc cgatgcaagt cccactggtc gcgcccgcgc caattgtcga
      481 gcagcatgcc caggcgtttc gagacctgtt tgagaaccgg tgtcagttcg atcactttca
      541 aaattatgtg acgggcttga tggtgttgga gaacaaaagt ctggccaata tcagtcggtg
      601 tgtggtggaa agcgcggata aaacgaatct gtcgcgattt ttcagccagg cgccgtggga
      661 agcaaaggaa atcaacgacc gacgggtgga atatatggtg gagcaaacgc aaggaaagcg
      721 gcgaggggtg aaagagtcgt gcctggccat tgacgacacg ttgtgcgagc atgtgggaag
      781 tttgtttgag tatgtggcgc ggcattatga tcacggtgaa aaccggtatc cattgggaca
      841 caatctggtg acgagtcatt tcgtgagtgg ggcggtgaga tttccaatcg atgtgagatt
      901 gtaccggcgc tatgaagagc aaactgattg ggaaaacacg gtcaggaagc attttccagg
      961 gcgggaaata ccgaccaaag ccaaggaacg caagcagttg cacaaggaag tggatgcgat
     1021 cttgttgacg gatgaggcgt ttcgccagcg gcacgaagcg ttccaaacca agattgagtt
     1081 ggcgatggaa ctcatcgagg atgcaatcga acgccgactc ccagttggcg tcctgctgat
     1141 ggatagctgg tatctggcgc cggagttgat caccaaattg aagttccatc acctggattg
     1201 ggtgagcttg ctcaaaaaga accgcaacct ggaaacggcc agtttcaccc tgcgtgacgg
     1261 ggagggaaag cccattgccc tggccggacc gcacattcaa gttgaggaac tggtcaaact
     1321 gattccacgc acggcatttc ggaaagaaac cgtggctgag cgggactttt ggtgttttgc
     1381 gcttaacctc cgcattcccg acctcgggcg ggttcgactg gttatttcct atggcacctc
     1441 cgatctgacc ggggcgtatg ccgttctggt ctcgaaccgc agtgactggt cggccaagaa
     1501 aatcattcaa acctatcttc aacgctggcc gattgaaacg ttttatcagg acagcaaagg
     1561 ccatctgggt ctggacgagt atcgcatgcg aactgccgaa gcctttcaaa aacattggtg
     1621 tctggtcttc gtggcgtatt cgtttctgca tttggattgt cttcccgcgt caccgccgaa
     1681 acgttcctcg cttgcccgcc ccatcaaaac cattggggag gtctgccgac ttcaaggcca
     1741 gcttttgctt cacaaactca ttctcaaagc ccatgacctg tttcaacagg gaagttctcc
     1801 cacccaggtg tttgcccaac tctttgccaa acttcaaccg gccctgattt gttgactttt
     1861 tggctttgta atctcaaaac acaacactcg agtgattaaa ttacttgact accttctcat
     1921 acgaggattt cattgcaaat tggtatcaaa gcagtcgtcg ggcgcggcca gcttgaaaca
     1981 aagctggtca aacaaacgac gtgtgtcttg ttacaaacgg ccttcctggt gccgccttgc
     2041 ctgttataca aatttggaat aacttgatcg ccttttcgag gagtatagtt gctgatcaaa
     2101 atgtatttcc ctcagccctt agccctcaac ccttagccca aactgatatt accggcgaag
     2161 ccacccgttg ctcgccttcc cgtgtcttta cgagtagaca ctggtaaacc aaagccgatg
     2221 aataacttgc cgaataaacc ccagttgcac cgatggcaga acgctgttct tgatgacctc
     2281 aaacaatacc tcatcaacaa aactcccttt ggaagccggg cgattggata tttgattttc
     2341 gaacatttga aatccgatcc aacctgggcc tatccagaac gctggttgga tgcactgaac
     2401 acttttggta aatcagtaat tgacccgcag actgtccaga tgatcgggta ccttgactgg
     2461 atggatcaac gacccagatt tggaggtgat gatttgtttg atgctacgtt ccgattagtg
     2521 caaactcctg aaggagaagc acttgactac atcatccgat tttgtgctga ggggg
//
LOCUS       DAOQDO010000773         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34802_length_2574_cov_2.832473, whole genome shotgun sequence.
ACCESSION   DAOQDO010000773 DAOQDO010000000
VERSION     DAOQDO010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34802_length_2574_cov_2.832473"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /gene="ptsP"
                     /locus_tag="PKZ53_12610"
     CDS             <1..1771
                     /gene="ptsP"
                     /locus_tag="PKZ53_12610"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNJ41327.1"
                     /translation="PAPATTVASAPPAPHPKNQPDDPNVLVGVSASPGIAVGTVFQLH
                     RQEVEVEEDGTTPDHEWIQLTQALEKGKAQLQALQAKLHGTADPAKAAIFAAHQELLE
                     DPDLLDITASMIDKGKSAAFAWKSAVNLHAERLAQLRNQLLAQRANDLRDVGQRVLVC
                     LTGVSPEPLKIPANSILIAEDLTPSDTATLDRKVVRGFCTSLGGATSHVAILARSLDI
                     PSIAGIDPKALTLANGTPVILDGSKGTLRLNPLPEEMERIQQRIQSREAKRKSDLSHA
                     HEPAVTRDGHHIEVVGNIGGLSDARNVPSLGGEGVGLLRSEFLFMERATAPTEDEQFQ
                     AYKEIAEAVGADRPLIIRTLDVGGDKPLAYLPIAREDNPFLGERGIRIGLDRPVILRT
                     QLRAILRASKFGKISVMFPMIAMLSELRDAKAIFLEECQALGVHPIPMGIMVEVPSVA
                     ILAEQFAREADFFSIGTNDLTQYTLAMDRGNPKMASKVDALNPSLLHLIDQTVRGAQA
                     HGRWVGVCGGIASDPHAVPILIGLGVTELSVSLPVIPAIKAQIRNLSVTECRELAGQA
                     LKLDNGAQVRELVPINEDSGEMK"
     gene            1768..>2574
                     /locus_tag="PKZ53_12615"
     CDS             1768..>2574
                     /locus_tag="PKZ53_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HNJ41328.1"
                     /translation="MSATAQAIETPSAFGPLIMLWRNAFSVLQKIGKAMMLPVSVMPV
                     AGILLGVGSAHLFFIPESVSNIMAQSGGAIFGNLPLIFAIAVAVGLTENDGVAAVAAT
                     VGFVVLLATMGTTAKMMNVETKPIMGIDSIETGVFGGILIGLIAAWAFNRFYRITLPS
                     YLGFFAGKRSVPIITAFAAIGTGIVMSFIWPPIGAGINTACRWAASERPDLAFSLYGF
                     VERLLIPFGLHHIWNVPFFFEVGKYLDPSTGKYVTGEINRFVAGDPTAGNM"
BASE COUNT          569 a          701 c          697 g          607 t
ORIGIN      
        1 accggcaccg gccaccaccg tggcatcggc gcccccggca cctcacccga aaaatcagcc
       61 tgacgaccca aatgttctgg tgggggtcag tgcctcacca gggattgcgg ttggcactgt
      121 ttttcagttg catcgccagg aagttgaagt cgaagaagac ggaaccactc cggatcacga
      181 atggatccag ctcactcagg cactcgaaaa aggcaaagcc caactgcagg cccttcaggc
      241 aaaacttcac ggcacggcgg atccggccaa agccgccatt tttgcggccc atcaggaact
      301 gctcgaagac cctgacctgc tcgatatcac cgcctcaatg attgataagg ggaaaagtgc
      361 ggcgtttgcc tggaaatcag ccgtcaacct tcatgctgaa cgcctggctc aactccgaaa
      421 ccagcttttg gcccaacgcg ccaatgacct gcgtgatgtt ggccagcgcg tgctggtttg
      481 cctgaccggg gtttcgcctg aaccactcaa aattccagcc aactcaatct tgattgctga
      541 agatttgacg ccatccgata ccgcaaccct tgatcgcaaa gtcgtgcgtg ggttctgtac
      601 ctcactcggc ggagcaactt cgcacgtcgc catcctggct cgatccctgg atattccctc
      661 gattgctgga attgatccaa aagcactcac gcttgccaat ggcacgccag tcatcctcga
      721 tggatccaaa ggcacgctcc ggcttaaccc cttgcctgag gaaatggagc ggattcaaca
      781 acggattcag tcccgcgaag ccaaacgcaa gtccgacctg tcccacgccc atgaaccagc
      841 cgtaactcgc gatggtcacc atatcgaagt tgtggggaat atcggcggcc tgtccgacgc
      901 cagaaatgtc ccatcactcg gtggtgaagg cgttggattg ctccggtctg aatttctgtt
      961 tatggaacgg gccacggcac ccaccgaaga cgaacaattt caagcctata aagaaattgc
     1021 cgaagcggtt ggggctgacc gccccttgat tattcgcacg cttgatgtcg gcggcgataa
     1081 accgctggcc tatcttccga ttgccaggga agacaatcct tttttgggtg agcgtggaat
     1141 ccgcattggc ctggatcgtc cggtgatttt acggacacaa ctacgggcga ttttgcgggc
     1201 gtcaaagttt gggaagatca gcgtgatgtt cccaatgatt gccatgctct cggaattaag
     1261 ggatgcgaaa gccatttttt tggaggaatg tcaggcactc ggagttcacc caatccccat
     1321 gggaattatg gttgaagttc catccgtggc aattctggcc gaacagtttg cccgcgaagc
     1381 cgattttttc tcgattggaa ccaatgattt aacccagtac acactggcca tggatcgtgg
     1441 caatcccaaa atggcatcca aagttgatgc attaaacccc agcctgctcc acctgattga
     1501 tcagacggta cggggcgccc aggcacatgg cagatgggtt ggcgtttgcg gtggaattgc
     1561 cagtgatccg cacgcggtgc cgattttgat tggactcggg gtcacagaac tcagtgtcag
     1621 cctgccggtc attccggcca tcaaagccca aattcggaac ctcagtgtga ccgaatgtcg
     1681 cgaactggcc ggacaggccc tcaaactcga caatggagcg caggttcgtg aactggtccc
     1741 tatcaatgaa gattcaggag aaatgaaatg agcgccacgg cacaggcaat tgaaacacct
     1801 tcggcctttg gtccattgat catgttgtgg agaaatgcct tttccgtcct tcagaaaatc
     1861 gggaaagcca tgatgcttcc cgtgtcggtg atgcctgtgg ccgggatttt acttggtgtc
     1921 ggcagcgccc atctgttttt cattcccgag agtgtttcca acattatggc ccaatccggc
     1981 ggggctattt ttggcaacct gcctctgatt tttgcgattg ccgtggcggt cgggttaact
     2041 gaaaatgacg gagtcgcggc ggtggcggca acagttgggt ttgtcgtgtt gctggccacg
     2101 atgggcacca cggccaaaat gatgaacgtt gagaccaaac ccattatggg tattgactca
     2161 atcgaaaccg gcgtctttgg cggtatcttg attgggttga ttgccgcctg ggcatttaac
     2221 cggttttatc gcatcaccct tccctcctac cttggttttt ttgccgggaa acgctcggtg
     2281 ccgatcatta ccgcctttgc ggcgattgga accgggattg tgatgagttt tatctggccg
     2341 ccaattgggg cgggcatcaa tacggcctgt cggtgggcgg ccagcgaacg acccgatctg
     2401 gcgtttagtc tctatggctt tgttgaacgc ctcctgattc cgtttgggct gcaccatatc
     2461 tggaatgtcc catttttctt tgaagttggg aaatacctgg atccttcgac aggaaagtat
     2521 gtgactggcg aaatcaaccg gtttgtggcc ggcgatccaa ccgccgggaa tatg
//
LOCUS       DAOQDO010000774         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34809_length_2573_cov_9.884035, whole genome shotgun sequence.
ACCESSION   DAOQDO010000774 DAOQDO010000000
VERSION     DAOQDO010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34809_length_2573_cov_9.884035"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1336)
                     /locus_tag="PKZ53_12620"
     CDS             complement(17..1336)
                     /locus_tag="PKZ53_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41329.1"
                     /translation="MSLRFKFLLYLILAHVLFAVIGARVFSKYGLWVFAVEGVFVISL
                     VIGLRLVGELFNTLNLIRSGAQFIKERDFTTKIREVGQPELDQLVAVYNRMIDSLREE
                     RLRLQENNYFLEKVLSASPSGILTLDFDNRISMVNPAAERLLQAEKSTLIGLTLEAVD
                     STFTRELGELKPGEARVIPYLGRRKVKCHKSEFIDRGFPRHFIVMEELTEELRQTEKA
                     AYEKLIRLMSHEVNNTIGAANSLLHSCLNYKDQLRPDDQEDYEMALQVVIDRTDQLNA
                     FMRSFADVVRLSQPKREACEIQVLLETVARLLKVECESRAIMLTWEIEDQLGRVNLDR
                     GQIEQVLVNLMKNAIEAIGENGRITIRTGKNLGRPFVTIEDTGAGFPPEVKANLFSPF
                     FSTKEHGQGIGLTLVQEILDNHGFDFALESEPGSPTRFTLFLSQKAG"
     gene            complement(1595..>2573)
                     /locus_tag="PKZ53_12625"
     CDS             complement(1595..>2573)
                     /locus_tag="PKZ53_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ41330.1"
                     /translation="VPTRDELDAQYDFHDLIGQDAKLLRILDLVGRVSATDASALITG
                     ESGTGKELIAEAIHRNSRRRNAAFVKVNLGGIAASLFESEMFGHVRGAFTDARTDRKG
                     RFEMANGGTIFLDEIGDLDPNSQVKMLRVLQDRTYEVLGSSVTRTVDVRVISATNRNL
                     PELVETGAFREDLLYRLNLIAIHLPALRERPSDIPLLARHFVQSMARVYRRSELSISD
                     GALNWLQTLNWPGNIRQLKQLLERTILITSKDTLEVEDFMVPVQMESKEKGKTTLPEV
                     GSMTLDDIEKAMILKTLKHHSGNLTKVAEALGLSRPALYRRLEKFGIQV"
BASE COUNT          628 a          704 c          612 g          629 t
ORIGIN      
        1 cccaacttct tcagccctaa ccagccttct ggctcaaaaa gagagtgaac cgggttgggc
       61 ttcccggttc actctccaaa gcaaaatcaa acccgtgatt atcgaggatt tcctgaacca
      121 gcgtgagtcc aattccctgc ccatgctctt tggtgctgaa aaatggtgaa aacaaattgg
      181 ctttcacttc gggaggaaac ccggcgccgg tatcttcaat cgtcacaaac gggcgtccca
      241 gatttttccc ggttcgaatc gtaatccgac cgttttcccc aatggcctca attgcatttt
      301 tcatcaggtt gaccaggacc tgttcgattt gcccccgatc cagattcact cgacccaact
      361 ggtcttcgat ttcccacgtc agcatgattg cgcggctctc acattcgact ttgagcaacc
      421 gggccaccgt ttccagcaat acctggattt cgcatgcctc acgtttcggc tgtgacagcc
      481 gcaccacatc ggcaaaactc cgcataaaag cgttgagctg gtcagtccgg tcaatcacca
      541 cctggagcgc catttcataa tcttcctggt catcgggtcg gagctgatct ttgtagttta
      601 gacaggaatg caggagtgaa ttcgctgcac caattgtgtt gttgacttca tggctcatca
      661 atcgaatgag tttttcgtaa gccgcttttt ccgtctggcg aagctcttca gtcagttctt
      721 ccatcacaat gaaatggcgt ggaaatcccc ggtcaataaa ttccgacttg tggcatttca
      781 cttttcgacg gccaagatac gggatgacgc gtgcctctcc gggtttcaac tctccaagtt
      841 ctctggtaaa agtcgagtca acggcctcca gagtcagccc aatcagggtt gatttctcag
      901 cttgcaacaa ccgctcggcg gcagggttga ccattgagat ccggttgtca aagtcgaggg
      961 ttaaaatccc agaaggcgat gccgacaaca ccttttccag aaagtagtta ttttcctgaa
     1021 gccgcagccg ctcttccctg agcgagtcaa tcattcggtt atagacggca accagttgat
     1081 cgagttcggg ctggccgact tcgcgaattt tggtcgtaaa gtcacgttct ttaatgaatt
     1141 gcgcaccaga ccggatcaaa ttgagggtgt tgaataactc tccgaccagc cgcagtccaa
     1201 tcacaaggga aatcacaaac accccctcca cggcaaaaac ccataaccca tattttgaaa
     1261 acacccgcgc cccaatgacg gcaaacagca cgtgggccag gatgagatag agcagaaatt
     1321 tgaatctgag ggacataaga tcagggttca gggttcaggg ttcaggattc aggattcagg
     1381 attcagggtt cagggtgtac cctgacgaca aaaaagcgac agaggaaaat catctgtcgc
     1441 tgcccaaatt gctttgaagg gagtatgtat atgacacggt ggcaaaaagt aagggcgccg
     1501 ttgctttgaa ccgcgcctct tcgaaaaccc ggaacccgga acccggaacc cggaacccgg
     1561 aacccggaac ccggaacccg gaacccttat gttttcagac ctgaatgcca aatttttcga
     1621 gccttcgata gagcgctggc cggcttaacc caagcgcctc agcgactttg gtcaggttgc
     1681 cgctatggtg cttgagagtc tttaaaatca tggctttttc gatgtcgtcg agcgtcattg
     1741 atccaacttc gggtaacgtc gtttttccct tctctttgga ttccatctga accggaacca
     1801 taaaatcctc gacttcgagt gtgtcttttg aggtaatcaa aatggttcgt tccagcaatt
     1861 gctttaactg acggatattt cctggccagt tcaaagtttg aagccagttt aaggccccat
     1921 cgctgatcga aagttcactc cgacgataga cacgggccat actttggaca aaatgccggg
     1981 caagcaatgg aatatctgat ggtcgttcac gtagcgccgg caggtgaatc gcaattaagt
     2041 tgagccgata gagcaggtct tcccgaaaag ccccggtttc gaccagttcc ggcaggtttc
     2101 gattggtggc tgaaatcacc cgcacatcca ccgtgcgggt cacgctcgaa cccagcactt
     2161 cataggttcg atcctgcagg acccgcagca ttttcacctg tgagtttgga tccaaatccc
     2221 caatctcatc caggaatatc gttccgccat tggccatttc aaagcggcct ttccgatcag
     2281 ttcgcgcatc ggtaaaggcg ccgcgaacgt gcccaaacat ctcgctttca aacaaactgg
     2341 ccgcaattcc acccaaattg actttcacaa acgcggcatt tcggcggcga ctgttgcggt
     2401 ggatggcttc ggcaatcagt tctttaccgg tcccgctttc accagtgatc agagccgaag
     2461 catctgtagc actgacccga ccgaccaggt ccagaatgcg aagtaatttc gcatcctgtc
     2521 cgatcaagtc gtgaaaatca tactgggcat ccagttcgtc gcgggttgga act
//
LOCUS       DAOQDO010000775         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34822_length_2573_cov_3.115171, whole genome shotgun sequence.
ACCESSION   DAOQDO010000775 DAOQDO010000000
VERSION     DAOQDO010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34822_length_2573_cov_3.115171"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="PKZ53_12630"
     CDS             complement(<1..2212)
                     /locus_tag="PKZ53_12630"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41331.1"
                     /translation="MSKPLFVRPLTVEERYQLEISVDADNHELQRRAQAILDSADGKT
                     AHDIASQLKTSAENVKRWLRQFNTGGLEGIQIRKRGPEPRFTEAQIEAIRALAARSPL
                     ILGYPFEHWSSQKLAEAARQQGIVDNISHVTIQKILTDLPNYRQSATASSLPARNGTT
                     ATPRTTSPQVNGSVRSRITQLESRLRRGHLLPVDEAAVRCALSEAFENIGGYAETLTA
                     VEHYDHAQPPDLSNDLLWQAHTRLRLGWGYTWHGNQPRALARMNEALRLFLDGGCLDG
                     VGAAHYAIGRTYAEINEFKIGRDRIWQGLRLLMKQIETGAEYKSWNPEWVEDTVSTWR
                     TRLMARMHLSLGVIDYCEGNFDAAKKNYVRALELATSAAHDRNLLGLVAMNLALIHDQ
                     VGERRDAKKYYEQAIEDFTKGGHEGFLARCHNNLGESLMHHGLWVQAAQHLEKALDLA
                     ERHQVRENEALTLITLAELRLLQGQIAVAEHHLSRAVQMLIDGDKWAEAYAQRIFGKI
                     LLAQGKEIDGLGKLQLALQMTTDIGDQYGQCLSYLELSDYYLHHEKLDQSESSLEMAR
                     ELLQSNSGFYAAGVAQRITGKLEAARHRYPEAQQHLLQSISIFTANDDQYEVGVSRLE
                     VGRLFQELQDFPQAHLHFDQAETLFVQLGAETAIGRVRELRQKLVTTPRATVTPANVA
                     IPPSESLLLQRLIEACNARELLLQELAAILQENFAAQQVVVWERDEYGAVRVLLA"
BASE COUNT          546 a          664 c          723 g          640 t
ORIGIN      
        1 gtgccagcag cacccgcacc gcgccgtatt catcgcgttc ccaaaccaca acctgttgag
       61 ccgcaaagtt ttcctgcagg atggccgcca gttcctgcaa caggagttcg cgggcgttgc
      121 aggcttcgat caatcgctga aggaggaggg attcggatgg tggaatcgcg acattggctg
      181 gcgtgacggt tgcgcgcggc gtggtgacca gcttctggcg caattcgcgt acgcgtccaa
      241 tggctgtttc ggcgcctaac tgcacaaaga gtgtttcagc ctgatcaaag tggaggtgag
      301 cctgagggaa gtcctgcagt tcctgaaaca accgaccaac ttccagacgg ctgaccccaa
      361 cttcatactg gtcgtcattg gcggtaaaaa tcgaaatact ttgcagtaaa tgttgctgtg
      421 cctcgggata gcgatgacgg gcggcttcga gttttccggt gatgcgttgg gccaccccgg
      481 cggcataaaa cccggagttg ctttgcagga gttcgcgcgc catttcaagc gatgactcgg
      541 attggtcaag tttttcgtgg tgaaggtaat aatccgacag ttcaaggtag gaaagacact
      601 gtccatactg gtcgccaata tcagtggtca tttggagcgc cagttggagt ttccctaacc
      661 catcaatttc ttttccttga gccagtaaaa tcttgccgaa gatgcgctgg gcataggctt
      721 cggcccattt gtcgccatca atgagcatct ggacggctcg tgaaagatga tgctcggcaa
      781 cggcaatctg accttgcaag aggcgcagct cagctagagt aatcagggtc agggcttcat
      841 tttcgcgcac ctgatgccgt tcagccagat cgagggcttt ttccaggtgc tgggcggcct
      901 gaacccagag cccatggtgc atcaggcttt cgcccagatt gttgtggcac cgggcgagga
      961 atccttcgtg accgcctttg gtaaaatctt caattgcctg ttcataatat tttttggcat
     1021 cgcgtcgttc gccaacctgg tcatgaatca acgccagatt catggcaacc agtccgagca
     1081 gattgcggtc atgggcagcg ctggtggcga gttcgagcgc ccggacataa ttttttttgg
     1141 ccgcgtcaaa atttccttcg caatagtcaa tcacgccgag gctgaggtgc atgcgtgcca
     1201 tgagtcgggt gcgccaggtg ctgacggtat cttcgaccca ctctggattc cacgatttat
     1261 attcggcacc agtctcaatc tgtttcatca agagccgcaa cccctgccag atgcggtcgc
     1321 gcccgatctt gaattcattg atctcggcat atgtccggcc aattgcatag tgagccgcgc
     1381 cgacaccatc cagacatcct ccgtccagaa acagccgcag ggcttcgttc atgcgcgcca
     1441 gggcccgggg ctgattgcca tgccaggtat atccccatcc aagccgcaag cgggtatgtg
     1501 cctgccacag caaatcgttg gacaggtcag gtggttgcgc atggtcataa tgttccaccg
     1561 ccgtgagggt ctcggcatac ccgccgatat tttcaaaggc ttcactgagc gcacagcgga
     1621 ccgccgcttc atcaaccgga agcagatgtc cgcgccggag tcgggattca agttgggtga
     1681 tccggctccg aaccgagcca ttgacctgcg gagacgttgt ccggggcgtc gctgtcgtgc
     1741 cattccgggc cggcagggaa gaggctgtcg ccgattgccg gtaatttggg agatccgtca
     1801 gaattttctg aatggtgacg tggctgatgt tatccacaat cccttgctgg cgcgccgctt
     1861 cggccagttt ttgggatgac cagtgctcaa atggataccc taaaatcaat gggctgcggg
     1921 cagccagagc acgaatggct tcaatctggg cttcggtaaa gcgtggctcc gggccgcgtt
     1981 ttcggatttg aatgccctcc agcccgccag tgttaaactg tcgcaaccag cgcttgacgt
     2041 tttcggcaga ggtcttaagc tgactggcaa tatcgtgcgc ggtttttcca tcggctgaat
     2101 ccagaatggc ctgggcacga cgctgaagtt catgattgtc agcgtcaacc gaaatttcca
     2161 attgataccg ttcctcgacc gttaagggac gcacaaaaag tggtttggac atcagtgcgt
     2221 ggtctcctgg gaagagatga aagtatttga gtgcggagag gggtaagaat gactggtcag
     2281 gaatgctttc gtcaatcgaa ttatgaatga tggattctga attctgaaac gttgaagagc
     2341 gattcttcca gacccagtct tgagtgtgtc agaatcaggg ccctgagagc tatatagttg
     2401 aaaaataagc agtactccaa tgttgcagcc ggctttggtt ttcactgcct tgcgctctat
     2461 cattcattct tcattcttta ttcttcattt tccattcttc attcttggat agtctggtgc
     2521 catttggtcg gtgtggaagg taattacgta cggagtgtct ctgtgtacag ttg
//
LOCUS       DAOQDO010000776         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34824_length_2573_cov_3.000794, whole genome shotgun sequence.
ACCESSION   DAOQDO010000776 DAOQDO010000000
VERSION     DAOQDO010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34824_length_2573_cov_3.000794"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKZ53_12635"
     CDS             <1..415
                     /locus_tag="PKZ53_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNJ41332.1"
                     /translation="VGCLTTTHPGYALGIAASFAAAASDTVSGEIGRVYGRHPRLITT
                     WKPAVTGENGAITLVGTLTGIVCSVGMATLAWTMGLLPSLLALGCVAGAGMAGNLIDS
                     FLGATLEHQGFLDNEGVNLCCTTSAALLVQLYWFS"
     gene            720..2495
                     /locus_tag="PKZ53_12640"
     CDS             720..2495
                     /locus_tag="PKZ53_12640"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNJ41333.1"
                     /translation="MTPAIAIVLVLLVLAVVLFSTERIPIDIVAIVIVVVLIVTKTLT
                     VDDAFAGFGNTIVITIAGLFIITGGLVKTGVVDTVGRYLHQMAGNDVTRLTLWVMVLA
                     GASAAVLKNTTTTAMLLPVVLGVADRAQIQPSKLLMPLAFGAILGGTCTLIGTSTNLA
                     VSGLLPRYGLVPFSMFELTPVGIGLVGAGLAYMIFLGAKLLPEHKSQASLADEYHIRE
                     YLSEVIVLPNSKLVGKTLADSNLRERLDLNVVGIVRDHTKEISPEADFRIASGDLLLV
                     QGRVEDILRIKNEVGIEIRADFKLSDKDLESGKIELFELMLLGSSDVVGSTFKASQFR
                     DRFGLTVLAINRHSTMVLSKISRLVLRFGDVLLVQGTRANVNRFVAEGNLLLSDVTDQ
                     SIRHGKRRFAIAALAVFLICSITQIVPLSVAVLVSVLILVATRAVLTQEMYEMIEWRL
                     LVLIAGMMSFGAAMEKTGTDKYLADWIVHHVGGLGGNAVLAGFFLLTVILTQPMSNQA
                     AALVVLPVAIKTAQTLGLDPRTFAITITYAASCSFLTPLEPACVLIYTPGRYRFFDFI
                     KIGFGLTVIMFVVVMWLVPYFWPLK"
BASE COUNT          553 a          568 c          713 g          739 t
ORIGIN      
        1 ggtcggatgt ttgacgacca ctcatcctgg gtatgcgttg gggattgcgg caagttttgc
       61 cgctgccgct tctgacacgg tttctggtga aatcgggcgg gtttatggtc gccatccacg
      121 attgattact acctggaaac ctgccgtgac cggtgaaaat ggcgccatta cgctggttgg
      181 aacactgacg gggattgtat gttcggtcgg aatggctact ctggcctgga caatgggctt
      241 actgccatca cttctggccc tgggatgtgt cgccggggct ggaatggccg gcaatctgat
      301 tgatagtttt cttggcgcga cgcttgaaca tcaaggcttt ctcgataacg aaggcgtcaa
      361 tctgtgttgc accacttcgg ctgccctgct ggtccaactg tactggttca gttgaatcga
      421 gtgaaatcgc gaaatagcca caaccacaaa acttgaggag ttgacagccc aaagctagaa
      481 atcaatgctg aagaacctgg ccactttgcc attctgtaat ttactggaat aatctctcat
      541 taaacaagcg ttgtgaaaag acagtttcct tttctgaaga gccgctgcag gtatcaactt
      601 cctgcagcgg tcatttttca atcgcatttg cgaaagactg actgattgaa aagatttgat
      661 ttcatcaggt cttgaatccc tatcctttaa cctgatttcc aaacaaagga gcccccgcca
      721 tgactccagc gattgccata gtactggtct tgttagtcct ggcggttgtt ttattttcaa
      781 ctgagcggat tccgattgat attgtcgcca ttgtgattgt ggtggtgttg atcgtgacca
      841 aaacgctcac cgtagatgat gcctttgccg ggtttggcaa cacaattgtg attacgattg
      901 ccgggttgtt tatcattacg ggagggctgg tcaaaaccgg tgttgttgac accgtgggcc
      961 ggtacctgca ccagatggct ggcaatgatg tgacccgatt aaccctgtgg gtgatggtgt
     1021 tggcgggtgc ctcagcggcg gtgctcaaaa atacaaccac cacggccatg ctcttgccag
     1081 tggtgcttgg tgtggcagat cgtgctcaga tacaaccttc taaattattg atgccactcg
     1141 ctttcggagc aattcttggc ggtacctgca ccttgattgg aacctcaacg aatctggccg
     1201 taagtggatt gctcccccgg tatggcctgg tgccattttc gatgtttgaa ctgacaccgg
     1261 ttggtattgg actggtgggt gccggcctgg catacatgat ttttcttggt gcgaaattgc
     1321 tgccagagca taaatcccag gcgtcactgg ctgatgagta tcacattcgc gaatatcttt
     1381 ccgaagtgat tgtgctgccc aattcaaaac tggttggaaa aaccctggca gattcaaacc
     1441 tgcgggaacg cctggatctc aatgtggttg gaattgtgcg cgatcacacc aaagagattt
     1501 ctcctgaagc cgatttcagg attgccagtg gcgatttgct gctggttcag gggagggtag
     1561 aagatatttt gcggattaaa aatgaagttg gcattgaaat cagagccgat ttcaaactga
     1621 gtgacaaaga tctcgaatct gggaaaatcg agttatttga attgatgttg ctcgggagtt
     1681 cagatgtcgt tgggagcacg tttaaagcat cgcaatttcg tgaccggttt ggattgaccg
     1741 tactggccat taaccgacac agcacaatgg ttttatcgaa aatcagtcgg ctggttttgc
     1801 gatttgggga tgtgctgctg gttcaaggca cccgtgccaa tgtcaaccgg ttcgtcgctg
     1861 aaggaaatct gttgttaagt gatgtgaccg accagtctat ccgccatgga aagcgtcggt
     1921 ttgcgattgc ggcactggct gtgttcctga tctgttcgat tacccaaatt gttccacttt
     1981 cggtggccgt tttggtcagt gtgctgattc tggttgctac gcgggcggtg ttgacgcaag
     2041 aaatgtatga aatgatcgaa tggcggttgc tggtcttaat tgcaggaatg atgagttttg
     2101 gagctgccat ggaaaagacg ggtactgaca agtacctggc ggattggatt gtgcatcacg
     2161 tcggaggatt gggtggaaac gccgtcctgg ctggtttttt tctgctgacc gtgattctca
     2221 cgcaaccgat gtcgaatcaa gccgcggccc tggtggtatt gccggtggcg atcaaaactg
     2281 cccaaaccct tgggctagat cctcgaacct ttgccattac tattacttac gctgcttcgt
     2341 gttcgtttct gaccccgctt gaaccagcct gtgtgttgat ttacacccct gggcgatacc
     2401 gattttttga tttcatcaag attggatttg ggttaacggt gattatgttt gtggtggtga
     2461 tgtggctggt gccctatttc tggccgctca aataaagatg aagaatgaag ccctggtccc
     2521 tggtgcttgg tgcttggtgc ttggtgcttg gtgcttggtg cttggtgctt ggt
//
LOCUS       DAOQDO010000777         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34849_length_2572_cov_2.791418, whole genome shotgun sequence.
ACCESSION   DAOQDO010000777 DAOQDO010000000
VERSION     DAOQDO010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34849_length_2572_cov_2.791418"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PKZ53_12645"
     CDS             <1..1584
                     /locus_tag="PKZ53_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNJ41334.1"
                     /translation="ARSLGFMDWDNQPDPFRRYAGAPFTELPLSETDESPACEALYTS
                     GSIPVSPVTIASIGLFFERSLAISAWKEYRGNRWALRINPSSGNLHPTEGYAVLPPVS
                     GIGDQPGVYHYAPREHGLECRTHFSIDHWNALSQGFPENTFFVGLTSIFWREAWKYGE
                     RAFRYCQIDLGHAIGTIAFAARSLGWEAIVLATPSDATLATLLGLDRKQEFHIEEPEH
                     PETLLAIVPSTTTNNLPDTIPVSPISEIAAGPWFGKANRLSKDHADWPIIGEVAAACQ
                     KPETPGFTTKLQTQPEHFQPVPPTVDLSAHEIFKQRRSAVSFDGVTSLTADQFFRILA
                     RTMPWAGSLPWNTFGGPVFVNLVLFVHRVEGLEPGLYFLVRNPAHEATLREKMLAGHL
                     WEKPQNCPDWLPLYLLDADKYQRIAARISCGQDIAGDSAFSLGMIAEFESPIARHGAW
                     LYRRLFWETGLIGQVLYLEAEAASVRGTGIGCFFDDPVHSLLGVGVQGMQFQSLYHFT
                     IGGPTDDGRLTTLPAYPNR"
     gene            1734..>2572
                     /locus_tag="PKZ53_12650"
     CDS             1734..>2572
                     /locus_tag="PKZ53_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41335.1"
                     /translation="MTAFRCMLGVWFVLGLGLSVQAAEPTIQVNEAGSKATTHSQFIH
                     LELNVENPGYREIPVRVKAMLLEVQAETEVTQTLPPGQSWVTLDLPLSGDLGSNQNLE
                     RLLTYRIHYRITPTADTGEDIATSGIFSVSLVARDVFRLRVLPQFRVTTWSEFCPKIL
                     AVHPISLTPVPHVQIKAEILSGTELISKSILITNQDGLVEPKFQFPAGFEKITEATLR
                     ISGKAGSTFTTRDFQIQIEQSPLILVTTDKLTYTAGEPLEINWFVARTDCTVVAGQKW
                     QIE"
BASE COUNT          578 a          706 c          658 g          630 t
ORIGIN      
        1 gcccgctcgc ttgggtttat ggattgggac aaccagccgg atccatttcg acgctatgcg
       61 ggtgctccgt tcaccgagct gccgctttca gaaaccgacg aatcgccggc ctgtgaggca
      121 ctttacacct ctggttcaat tccggtttca ccagtcacca tcgccagcat tggtctattt
      181 tttgaacgct cactggcaat ttcggcctgg aaagaatatc gcgggaatcg ctgggcgttg
      241 cgcatcaatc catcaagcgg caatttgcac ccgacggaag gctacgcagt actccctcca
      301 gtgtctggaa tcggcgacca gccgggtgtg tatcactatg ccccacgcga acacggtctg
      361 gaatgccgga cccacttttc cattgatcac tggaacgcat tgtcccaggg ctttcccgaa
      421 aacacctttt ttgtcggatt gacttcgatt ttctggcgcg aagcctggaa atatggcgag
      481 cgtgccttcc gctattgtca gattgacctt ggccatgcga ttggaaccat tgcctttgcg
      541 gctcgctcgc ttggctggga agccattgtt ctggcaactc ccagtgatgc aacgctggca
      601 acattgcttg gattggaccg caaacaggaa tttcacatcg aagagccaga gcacccggaa
      661 actctgctgg cgattgtgcc ttcaaccaca acgaacaacc tgcctgacac aattcccgtt
      721 tctccgattt cagaaattgc ggctggtccc tggttcggaa aggccaaccg gttgagtaaa
      781 gaccacgccg actggccgat cattggcgaa gtggccgccg cctgtcagaa accggaaact
      841 cctggattca ccaccaagtt gcagacacaa cctgagcatt ttcaacctgt tccacccacg
      901 gttgaccttt cagcccacga gatcttcaaa caacgccgaa gcgccgtctc ttttgatgga
      961 gtgaccagtc tcaccgcaga ccagtttttc cgaatcctgg cacggaccat gccctgggca
     1021 ggatcactgc cgtggaacac atttggtggc ccggtctttg tcaatctggt gttgtttgtg
     1081 catcgggtcg aaggacttga acccggactg tattttttgg ttcgcaatcc ggcccatgaa
     1141 gccacgctcc gggaaaaaat gctggctggg cacctgtggg aaaagccaca aaactgcccc
     1201 gactggctcc ctttgtattt acttgacgcc gataaatatc agcgaattgc cgcccggatt
     1261 agttgcggcc aggacattgc cggagacagc gcgtttagtc ttgggatgat tgccgagttt
     1321 gagtcaccaa ttgcccggca cggcgcctgg ttgtatcggc gactcttttg ggagactggc
     1381 ctgattggac aggtgctcta tctcgaagcc gaagccgcta gcgtacgcgg caccgggatc
     1441 ggctgtttct ttgatgatcc ggtccattcg ctgctgggtg tcggggtaca agggatgcag
     1501 ttccaatcgc tctaccattt cacaattggt gggccgacag atgatggccg gctgacaacc
     1561 ctgcccgcct atccaaaccg gtgaaagtgg cgtcaagccg ccccacatca caaaactcaa
     1621 caattccccg aagttctgct attgtgctct tctcagtgcg cgtttgcgct tcgaagccgc
     1681 aattgcaggc ggttggcccg gtagaacttt aaatagttgg gagaggtgag tttatgacag
     1741 ccttccgatg tatgcttggt gtgtggtttg tgctgggatt gggtctatca gtccaggcgg
     1801 ctgaaccaac gattcaggtc aacgaagccg gttcgaaagc caccacccac agtcagttta
     1861 tccatctgga attaaatgtc gaaaacccag gataccgcga gattccggtt cgggtgaagg
     1921 ccatgctttt agaagttcag gcggaaaccg aagtcaccca gacactgcca cccggacagt
     1981 cctgggtaac gctggatctt cccctgtcag gtgatcttgg gtccaaccag aatctggaac
     2041 gcttgttgac gtatcggatt cactaccgga ttactcccac tgccgacacc ggagaggaca
     2101 tcgccaccag tggtatcttt tcagtatcac tcgtcgctcg ggatgtcttt cgattgcggg
     2161 ttttgcctca gtttcgagtc accacctggt cagaattttg tccaaaaatc ctggcagttc
     2221 acccaatttc actgactccg gtgccgcatg tgcaaatcaa agcagaaatc ctttccggca
     2281 ctgagctcat ttccaaatca atcctcatta caaatcagga tggtttggtt gagccaaagt
     2341 ttcaatttcc ggctggcttt gaaaaaatca ctgaagccac acttcggatt tcggggaaag
     2401 ctgggagtac ttttacaaca cgtgattttc agatccaaat cgaacagtcg ccattgatcc
     2461 tggttaccac tgataaatta acctacaccg ccggagaacc tctggaaata aactggtttg
     2521 tagctcgcac tgattgtact gtggtcgcag ggcaaaaatg gcaaattgaa tg
//
LOCUS       DAOQDO010000778         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34899_length_2570_cov_3.801193, whole genome shotgun sequence.
ACCESSION   DAOQDO010000778 DAOQDO010000000
VERSION     DAOQDO010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34899_length_2570_cov_3.801193"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKZ53_12655"
     CDS             <1..228
                     /locus_tag="PKZ53_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNJ41336.1"
                     /translation="VTVRWQDNSTNETNFIVERAPKSTGRFTQAASLVANTTSFIQNL
                     VPGTYLYRVRAYNRTTKKYSAYSNTAEVTVR"
     gene            581..1828
                     /locus_tag="PKZ53_12660"
     CDS             581..1828
                     /locus_tag="PKZ53_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ41337.1"
                     /translation="MNTARPGAKKIGLMILVAFCLGYATFALQSYLSRPKPKKQRKPS
                     QNQNSKQPSWARNNPSGRKNKPIPADLTVPADEDPVFLETVLQTIFPQGRKLLSDEEK
                     TLEILRYVASYLKDKTTSKSRATDILREGFSFCGGKGQVFVILARKVGIPARIVNGVY
                     LPTLSSHVATEVFYAGNWHFIDPTFGIFFYSRPEYDQQGTIISFQELVLDASFGTPFK
                     VVKEPWKGEYTNRIRSFGVAHIEPDFLEYKYGSSFISLYREELAGAYPIFSGDSSFVS
                     YPVDADFEKQDAFFLGEINQSGDDLIRFRARNSAEYGTRYFGNWAIGQGAPATCHTWL
                     IHSPPQSLIQIEYYGLQSSPPKLKLFPLRTAHLVETRYEGAKTTFLVRVIDHDAIVSV
                     NCPKGIFEIDAMKAARIRRKAEG"
     gene            complement(2006..>2570)
                     /locus_tag="PKZ53_12665"
     CDS             complement(2006..>2570)
                     /locus_tag="PKZ53_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNJ41338.1"
                     /translation="ENIHRVQQSSLSPLKINCVVMRGVNDHEILDFVNFARETRVAVR
                     FIEYMPLNGPDQWRPEVMVSGDEILERIQHQFQLIPVAPVSPSETALRFRFADGGAGE
                     IGIIASVTRPFCNACSRIRLTADGKLKTCLFAHQEYDLRGRLRAGASRSVLKDLICNA
                     VFHKGSGHTINSPEFLAPARTMSCIGG"
BASE COUNT          664 a          599 c          646 g          661 t
ORIGIN      
        1 gtaaccgttc gatggcagga taattcgacc aacgaaacca attttatcgt ggaacgggcg
       61 ccaaagagta ccggacggtt tacccaggcg gcttcactgg tggccaatac cacgtcgttt
      121 atccagaatc tggtaccggg cacctacctg taccgggtac gggcatataa tcgaactacc
      181 aaaaagtatt cggcttattc aaacacggct gaagtcacag taaggtaagg gcgattccaa
      241 attttttggc ttgcaatttt gctgagatga gagagatagt tggtttgcga tttttcgtgg
      301 ttccctcatt cggttaagtt ttacgcttcc aatctattca acagaatctt gtcctgacgg
      361 tgaaatcaga cgtcactttt tcggaggtgg ctccactgat attggccact ctttcaaagc
      421 tggtggctga tgtcagaaaa gccattgccg gagagattgt ctacgattgg cattgtgatg
      481 atttcacccg agattcagga ttcagagaaa tacaagtatt tcaaaggttt agccttgaaa
      541 taaaccaaaa cagactgttg atgattgact gtaaacttgt atgaacactg cccggcctgg
      601 cgccaaaaaa ataggtttga tgatcctggt tgctttttgt ctgggatatg caacctttgc
      661 cttgcaatcg tatctctcac gtcccaaacc aaaaaagcag cggaagccca gccaaaacca
      721 gaattcaaaa cagccatcct gggccagaaa caatccttca ggccggaaaa ataaaccgat
      781 cccagcggat ttgaccgtgc ctgccgatga agatccagtc tttttggaaa cggttttaca
      841 aacgatcttt ccacagggaa gaaagctgtt gtcagatgaa gaaaaaacgc tggaaattct
      901 gcggtatgtt gccagttacc tcaaagataa gacgacctca aagtctcggg cgactgatat
      961 tttgcgggag gggttttcat tttgtggtgg caagggacag gtgtttgtga tcctggctcg
     1021 caaggttggc attccagccc gaattgtgaa tggggtgtat ttgccaacct tgagcagtca
     1081 tgtcgccacc gaagtctttt atgccgggaa ctggcatttt attgacccca cgtttggaat
     1141 tttcttctat tccaggcccg agtatgacca gcagggaacc atcatttcat ttcaagaact
     1201 ggtgcttgat gccagttttg gcacgccgtt taaggtggtc aaagaaccct ggaaaggtga
     1261 gtacacaaac cggattcggt catttggtgt tgcgcacatc gaacccgact ttttggaata
     1321 taaatatgga agttcgttta tttccttgta tcgtgaggaa ttagctggcg cctacccaat
     1381 tttttccgga gactcaagct ttgtttccta ccccgtagat gctgatttcg aaaaacagga
     1441 cgccttcttc ctgggtgaaa tcaatcagtc aggtgatgac ctgattcgat ttcgagccag
     1501 gaattccgct gaatatggaa cccgttactt tgggaattgg gccatcggcc agggagcacc
     1561 cgcaacgtgc cacacctggc tgattcattc accgccgcaa agcctgattc aaatcgaata
     1621 ctatggcttg cagtcatcac ctcccaaact caaattgttt ccacttcgaa ccgctcatct
     1681 ggttgaaacc cggtatgagg gagcgaaaac aacgtttctg gttcgggtca ttgaccacga
     1741 cgccattgtt tcagtgaatt gcccaaaagg tattttcgag attgatgcga tgaaagccgc
     1801 aaggataagg cggaaggctg agggttgaag acggcgggtt gaagaaatcg ggctgaaaac
     1861 aacgggcaga agatttcggg cagaagacat tgggcagaag aagtttgggc agaagacggc
     1921 gggctcaggg gattgaaccc gtttttttct gccctgagct tgcgaatctt cagccctcag
     1981 cccgtcgtct tctgccctgg ttttctcagc ctccaataca ggacatcgtg cgtgctggag
     2041 ccagaaattc tggtgagttg attgtatgac cagatccttt gtggaaaaca gcgttacaaa
     2101 tgaggtcttt taacacactt cgtgaagctc cagcccggag ccgtccgcgc aggtcgtact
     2161 cttgatgggc aaacaggcag gttttgagtt ttccatccgc cgtgagtcga atccggctgc
     2221 aggcattaca aaacggacgg gtgaccgaag caatgattcc gatttcaccg gcgcctccat
     2281 cggcaaaccg aaaccgaagc gcggtttcac ttgggctgac cggcgccaca ggtatgagtt
     2341 gaaactggtg ttgaatgcgc tccaggattt cgtcacctga aaccatcact tcaggccgcc
     2401 actgatctgg cccattgagc ggcatatatt cgataaatcg gacagcgact ctggtttcac
     2461 gggcaaagtt cacaaaatca aggatttcgt ggtcattgac accccgcatc acgacacaat
     2521 tgattttgag tggagacaaa ctggattgct ggacccggtg aatattttca
//
LOCUS       DAOQDO010000779         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_34912_length_2570_cov_2.207157, whole genome shotgun sequence.
ACCESSION   DAOQDO010000779 DAOQDO010000000
VERSION     DAOQDO010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34912_length_2570_cov_2.207157"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKZ53_12670"
     CDS             complement(<1..581)
                     /locus_tag="PKZ53_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41339.1"
                     /translation="MLTAINYYHQLIQNDPQSALRQCDLLEQKFIERNIMFAGKPSPF
                     HLRPHLMSSVVRQNLENAAHTVLEASLKAELGLFGGDAEKLYDALFVSENERILLRVE
                     PGYRERVIWSRLDAFLGAGDDDIKFLEFNNDAPAGIGYSALMAQSFLELPIMEEFQTR
                     YSVAAEDARPQLLKALLDTYKVWGGSEHPQIAIV"
     gene            complement(601..1404)
                     /locus_tag="PKZ53_12675"
     CDS             complement(601..1404)
                     /locus_tag="PKZ53_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41340.1"
                     /translation="MKFSRRIFLQTGVLAALGSGLPDRKNTAFGAQHQHSGDHPAVHG
                     MVVMGEKTIFLSHLPLFKTATYNSPHDYQVILQATFRHPTQNAIQIYQKDRKQTKTRL
                     YTLEPEKFVLPTLAGPVSDVPHRTEFQGTLYRGHFERGGEPILKDLTVQVSRVVHFRK
                     FDPQAVKPNQLQYLMFGNTSEQFLAHFISKPPDFDQILAVRSLTPKISEADLRQCPIL
                     SIADRPDAAASKLAEGFHGPIRLQTGKTAVTGQIAIQTEIYFEAGELSP"
     gene            complement(1512..2225)
                     /locus_tag="PKZ53_12680"
     CDS             complement(1512..2225)
                     /locus_tag="PKZ53_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526802.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNJ41341.1"
                     /translation="MKIHSLQHVPFEDLASIADWAQARGHQVSITHLYQNTSLPDVSD
                     LDVLVVLGGPMNVDDEHLFPWLAAEKRLIAQAIQAGKLVLGICLGSQLIASVLGARVH
                     PNIHKEIGWFPVSLTSEGSQSPILAGFPATFPAFHWHGDTFELPPGAVHLAQSEACQH
                     QVFSFGHNVLGLQFHLESTIESLTRMLDHEADDLTDGPFVQTQDAILTAGHHVETSVR
                     LMHHLLDTFTASTSESQAH"
     gene            complement(2222..>2570)
                     /locus_tag="PKZ53_12685"
     CDS             complement(2222..>2570)
                     /locus_tag="PKZ53_12685"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNJ41342.1"
                     /translation="GLLAYAPCCIGLIASLAFIFTEKNNRFVRFHAVQSLFLHGVFFV
                     LGIGFSVLFTILGLIPVINVIAAIASIPLSLLLLAVQLGVTIYLMIKAYGGEMTKLPT
                     IGDLAEKNINLGL"
BASE COUNT          643 a          594 c          712 g          621 t
ORIGIN      
        1 acaatcgcaa tctggggatg ttcagagccg ccccagactt tgtaggtatc aagcaaggct
       61 ttgagcaact gtggacgagc gtcttcagcc gcgacactgt agcgggtctg gaattcctcc
      121 ataatcggca attcgagaaa actttgggcc atcaacgccg aatagccgat tcctgccggg
      181 gcgtcattgt tgaattccag aaacttgatg tcatcgtcgc cagcgccaag aaacgcatcg
      241 agccgcgacc agatcacgcg ttcgcggtac cctggctcaa cccggagcaa aatccgttca
      301 ttttcactca caaacagagc gtcatagagc ttttccgcat cgccgccaaa gaggccaagt
      361 tcggctttga gggaagcttc aagcacggta tgtgctgcat tttcaaggtt ttgccggact
      421 actgatgaca tcaggtgggg acggagatga aacggcgatg gtttcccggc aaacatgatg
      481 ttgcgctcga taaatttttg ctcaagcaga tcacattgtc tgagcgctga ttgtgggtcg
      541 ttttgaatga gctggtgata gtaattgata gctgtcagca taggttttat tccaaaagat
      601 ttaaggagag agttcgccgg cttcgaaata aatttccgtt tggatggcaa tctgtccagt
      661 gactgctgtc ttaccggtct gaagccggat tgggccgtga aagccctcgg cgagtttgct
      721 cgccgccgca tcaggtcggt cagcaatgga gagaatcgga cactggcgta agtcggcttc
      781 tgaaattttt ggtgtgagcg accgaaccgc cagtatctgg tcaaagtccg gtggtttgga
      841 aatgaaatgg gccaggaact gttcgctggt attgccaaac atcaggtatt gcaactggtt
      901 gggcttgact gcctgcgggt cgaatttccg aaaatggacc acgcgcgaga cctgaaccgt
      961 gaggtctttt aaaatcggct cgccgccgcg ctcaaagtgt ccacgataga gtgtgccttg
     1021 aaattctgtc cgatgcggga catctgaaac tggtcctgcc agtgtcggga gcacaaattt
     1081 ctcaggttcc agcgtgtaga gtcgggtttt ggtttgcttg cggtctttct ggtaaatctg
     1141 gatggcgttt tgggttgggt gacggaacgt agcctggaga atgacctgat aatcgtgggg
     1201 cgaattgtac gtggcggttt taaagagcgg caggtgcgag aggaaaatag tcttttcacc
     1261 catgaccacc atgccatgca ccgccgggtg gtcgcctgaa tgctggtgct gcgcaccaaa
     1321 ggcagtgttc ttcctgtcag gcagacccga tccaagtgct gcgagcacac cggtttgaag
     1381 gaaaattcgg cgggaaaatt tcataagtac tttatcaacg gtttattgag aaattgattc
     1441 tggtaagtat ttgatttttt tcgtgttttt cgtggttaaa actgtctttg gatttctggc
     1501 gggtgcttat ctcaatgagc ctgggattca gatgttgaag ctgtaaaggt atcaagcaag
     1561 tggtgcatca acctcacgct ggtttcaacg tgatgaccag ccgtcaagat tgcgtcttgg
     1621 gtctgaacaa acggaccatc cgtcaggtca tcagcttcgt ggtcgagcat ccgagtcagg
     1681 ctttcaatgg ttgattccag atgaaactgg aggcccagca cattgtgacc aaaactgaat
     1741 acctgatgct ggcaggcttc gctctgtgcc agatgaactg cccccggagg tagttcaaac
     1801 gtgtcgccat gccagtgaaa cgccgggaag gtggctggaa atccagccag aattggtgac
     1861 tggcttcctt ccgatgtcag tgaaaccgga aaccagccga tttctttatg gatattggga
     1921 tgaaccctgg ctccgagcac gctggcgatt aattgcgagc caagacaaat gccaagcaca
     1981 agttttccag cctggatggc ctgtgcaatc aatcgcttct cggcagcgag ccagggaaag
     2041 aggtgttcgt catccacgtt catcgggcca ccaaggacaa ccagcacatc gaggtcagaa
     2101 acatcgggaa gggaggtgtt ctgatagagg tgcgtgattg aaacctggtg gccccgagcc
     2161 tgtgcccagt cagcgatact ggccagatct tcaaatggaa cgtgttgcag cgaatggatt
     2221 ttcataatcc gagattgata tttttctcag cgagatcacc gatggtcggc agtttggtca
     2281 tttcaccgcc ataggctttg atcatcaggt aaatggttac gccaagttgc acggccagca
     2341 gtaggaggct caatggaata gaggcaatcg cggcaataac gttgatcacc ggaataagcc
     2401 ctaaaatggt aaacaaaact gagaaaccaa ttccaagcac aaagaaaacc ccatgcagga
     2461 acaaagattg gacggcatga aaccgaacaa aacgattgtt tttctcagtg aaaataaacg
     2521 ccagtgaagc gatcaagccg atgcagcaag gggcatatgc cagcaaccct
//
LOCUS       DAOQDO010000780         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35028_length_2565_cov_2.856574, whole genome shotgun sequence.
ACCESSION   DAOQDO010000780 DAOQDO010000000
VERSION     DAOQDO010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35028_length_2565_cov_2.856574"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKZ53_12690"
     CDS             complement(<1..571)
                     /locus_tag="PKZ53_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ41343.1"
                     /translation="MTNHNQLSHPVHPQFTVAQSLSRVEPSSDQPQSIHDEEFILRAL
                     VEGTATAIGDDFFRSLVQQLALVLGVRYAFVAEFAEVNTRARTLAYFGNQEFRPNIEF
                     LLAGTPCEDVVRGTVCHYPERVQQLFPADKALIHLGVESYLGVPLLDANGEVLGHLAV
                     FDDKPMPKESRLMSIFRIFASRATAELDRK"
     gene            589..957
                     /locus_tag="PKZ53_12695"
     CDS             589..957
                     /locus_tag="PKZ53_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41344.1"
                     /translation="MQTGNLDRKETIVTGKTFGEDVHSLLPPEELIWNQPQLIDFGEV
                     QEDNWGNSEYRKISFGATLNSLHKSIRLDFVIQPAWFVICTLGIVRKIAVEIQWLRIS
                     FDFFPGFSGGPIRVRRRQYG"
     gene            950..2440
                     /locus_tag="PKZ53_12700"
     CDS             950..2440
                     /locus_tag="PKZ53_12700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ41345.1"
                     /translation="MAENQKSTQAQSISQSISQFGIELDAFQAEQRKLQRTYFLNVIY
                     IPSMRLLGYILVSVVVFLHNRYLLPGMGDVPALWFAGISTLHGLVSWIILFLFFSRSK
                     FIHLGDLFLWLDFVIFGLGVYCSGGDKSWLFMIMMIRAGDQNVTTLKRTTIFAAGGWV
                     SYLVLMGYLELVEHRQVNWPGEFTKALFMIAASFNAVMIARMSERLRISVTSAIRMAR
                     GFIQEMRHQTEELELARASLEKLYLREQEVTRTLTELNQMKTNFLIVTSHEMRTPLTV
                     IKGYNEALLNQFFGSLSDAQKKSLEACQHVVDRLTMTLEDILEMLKINEGHIALKLRT
                     FELIELITQVSQDFVPFLEKRHQHLSFEPVDRVDLHADQEKIHLILLNLLQNAIKFTP
                     DGGKITIQVQMEAPNVHILISDTGIGVESSEIEKIFDKFYINDDPSYHTSGKFQFMAR
                     GTGLGLSIAKSYVEAHGGKIWAESQGRGHGSCFHVILPLNQEKNDE"
BASE COUNT          630 a          583 c          650 g          702 t
ORIGIN      
        1 gttttcggtc gagttctgcg gtggcacggg aggcaaaaat gcggaaaatt gacatcaggc
       61 gcgattcttt cggcattggc ttgtcatcaa agaccgccag atgacccagg acttcgccgt
      121 tggcatcaag cagcggcaca cccagatagc tttcaacccc caaatgaatc agcgctttgt
      181 cagccggaaa caactgctgt acccgctctg ggtagtggca gactgtaccg cgtaccacat
      241 cttcacaggg ggttcccgca agcaaaaatt caatattggg ccgaaactct tgatttccaa
      301 aataggccag ggttcgggca cgagtgttga cctcggcaaa ctcagccaca aaggcatacc
      361 tgaccccgag aaccagagcg agttgttgca ccagtgatcg aaaaaaatca tcaccaattg
      421 cggtggcggt tccctcaacc agtgcccgga ggataaattc ttcatcgtga attgattgag
      481 gttggtcaga cgagggctca acgcgggaga gagattgagc caccgtgaat tgaggatgaa
      541 ctggatgtga aagctggttg tggttggtca tatgggcctc cgccggcaat gcaaacaggc
      601 aatcttgatc gaaaagaaac tatcgtcacg ggcaaaacct ttggtgaaga tgtccactca
      661 cttctaccac cggaagaact tatttggaat cagccgcaac ttattgattt tggcgaagtt
      721 caagaagata attggggcaa ctcagaatac cggaagatct cttttggcgc gaccctcaat
      781 tcacttcaca aatcaattcg cctcgatttt gtgattcaac ctgcctggtt cgtcatttgc
      841 actttgggaa ttgttcggaa aattgctgtt gaaatccagt ggttgcggat ttcattcgat
      901 tttttccctg gattttcagg cgggccgatt cgtgtgcgga gaagacaata tggctgaaaa
      961 ccaaaaatca acccaggcgc aatcaatttc ccagagcatc tcacaatttg gaattgagct
     1021 tgatgcgttc caggctgaac agcgaaaact acagcgaacg tactttttga atgtcatata
     1081 cattccgtca atgcggcttc tggggtacat tctggtgtcg gttgttgtgt ttttacacaa
     1141 tcggtatttg cttccaggaa tgggagatgt gccggcgctc tggtttgcag gaatttcgac
     1201 cctgcacggt ctggtttcct ggatcatttt gtttctgttt ttcagtcgct cgaaatttat
     1261 tcatttaggc gatttgttcc tctggctgga ttttgtcatt tttggactcg gcgtgtactg
     1321 ctcaggtggt gataaaagct ggttgtttat gattatgatg attcgcgccg gagatcaaaa
     1381 tgtcaccact ttgaagcgga cgacgatttt tgccgcaggt ggctgggtga gttacctggt
     1441 gttgatgggg tatctggaac tggttgagca ccgtcaggtc aactggcccg gagagtttac
     1501 caaagccttg tttatgattg cggccagttt taatgccgtg atgattgccc ggatgtcaga
     1561 acggttgcgg atcagcgtca ccagtgccat ccgaatggca cgtggattta tccaggaaat
     1621 gcgacatcag accgaagaac ttgagttggc gcgcgccagc ctggaaaagc tgtatttgcg
     1681 cgaacaggaa gtcacccgta ccctgaccga actcaatcaa atgaaaacca actttctgat
     1741 tgtgacttct cacgaaatgc gaacgccgtt gaccgtgatc aaagggtaca acgaagccct
     1801 gctcaatcag ttttttggtt cgctctctga tgcccagaaa aaatcgctcg aagcctgcca
     1861 gcacgtggtt gaccggctca ccatgacgct cgaagacatt ctggaaatgc tcaaaatcaa
     1921 tgaagggcac attgccttaa agctgcggac ctttgaactg atagagttaa tcactcaagt
     1981 gagccaggat ttcgttccat ttttggaaaa acgccatcag catctttcct ttgaacccgt
     2041 tgaccgggtt gacctgcacg ccgaccagga aaaaattcat ctcatcctgc tcaatctgct
     2101 gcaaaatgcg atcaagttca cccccgatgg cggcaaaatc accatccagg ttcagatgga
     2161 agcgccaaac gtccacattc tcatttctga taccggaatt ggggttgagt cctcagaaat
     2221 tgaaaagatt ttcgataaat tttatatcaa tgatgacccg tcctatcata cgtcgggtaa
     2281 atttcagttt atggcgcgtg ggacgggcct tggattatca attgccaaaa gttatgtcga
     2341 agcccacggt ggaaaaatct gggctgaaag ccagggcaga ggtcacggaa gttgttttca
     2401 cgtcattttg ccgctcaatc aggaaaagaa tgacgaatga agaagcactg gttcttggct
     2461 cctggttctt ggttcttggt ttttggttct tggttcttgg ttcttggttc ttggttcttg
     2521 gttcttggtt cttggttttt ggttcttggt tcttggttct tggtt
//
LOCUS       DAOQDO010000781         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35029_length_2565_cov_2.689243, whole genome shotgun sequence.
ACCESSION   DAOQDO010000781 DAOQDO010000000
VERSION     DAOQDO010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35029_length_2565_cov_2.689243"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKZ53_12705"
     CDS             complement(<1..351)
                     /locus_tag="PKZ53_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41346.1"
                     /translation="MPLTDAQLVSQAQAGDFAAFEELVNRHEQRLYRLALRITRNVSD
                     AEDVLQTALMQALESLGDFRGDAAFGTWVTQIATHEALKVLRRRATHPDVPLTLGNDD
                     DDQPIPFPEFIADWR"
     gene            916..1551
                     /locus_tag="PKZ53_12710"
     CDS             916..1551
                     /locus_tag="PKZ53_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ41347.1"
                     /translation="MHLLHPATTNEDLLQLMLTGDETALETLYARLHPVIYRFAFRMG
                     GSAALADDVSQETFLQLIQEGARFDPSRGNATAYLLGIARHVLWNRLDRDQKFVPLAE
                     TSDESDLSPTTSIDLTTPLTSVLQHETVENVRLAITGLPAHYRDVVVLCDLQELSYQD
                     VATILGCPIGTVRSRLHRAHDVLCQRLFQFRNASLDVSVQLHPAERNHRCA"
     gene            1711..>2565
                     /locus_tag="PKZ53_12715"
     CDS             1711..>2565
                     /locus_tag="PKZ53_12715"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNJ41348.1"
                     /translation="MKATKLLLFVALVSFFIAIQGMKTPVLKAQTDDPALQGTGVEAQ
                     TGFDHESNGFLDQTSFEEARDGFDAVEDLEDGVGPVFNGKSCGECHGNPISGGNSQVA
                     VIRAGHFDGTSFLDHPGGSLIHSRAIDPRIQERIMPGNETRSFRMSLSILGDGFIEAI
                     DDNALLAIAQAQKTATNGKIQGQAILVPVLEAPGVSRVGRFGWKAQHASLLSFAGDAY
                     LNEMGITTPMFPVENTANGRDVSQFDTVADPDEADNDDLELFANFMRSTKAPSRDERM
                     MNAPEGRNG"
BASE COUNT          601 a          655 c          655 g          654 t
ORIGIN      
        1 gcgccagtca gcaataaatt ccggaaatgg aattggctgg tcatcgtcat cgttccccag
       61 agtcaacggg acatctggat gagttgcccg ccgccggagc actttgagtg cttcgtgggt
      121 ggcgatttgg gtaacccagg tgccaaaagc ggcgtctcct cgaaaatctc ccagcgactc
      181 aagggcctgc atcaatgcgg tttgcagcac atcttccgca tcagaaacgt tgcgcgtgat
      241 gcgcagcgcc agccggtaga gccgttgctc gtgccggttg acgagttctt caaaggctgc
      301 aaagtcacca gcctgtgcct gagaaacaag ttgggcgtct gtaagtggca taagtgaaga
      361 gcgagtagcg agtcgtgaat ggcgaatagc gggtagaatg ccacgtcaaa tgtaggccat
      421 ttatcgccag agtaaaaggc aatttgtgaa cgcgttcaca acgggttaaa taagagaaat
      481 caaatgcttt aggtggtcct agagcttttt ttatagattt tcacgagctg gttcagtgtc
      541 tcgtcttcag aaaaaagatt tttctttggt tcaactcgtc actttccgtg ctggaaggcg
      601 ttcctctgaa ccgaaatcct gaggcaggtt gcccgaaccc tgatgttttc agtctctttt
      661 ggttttttcc tgggtttttt gaaaaaaaat tggaaccaat cggctatgat ggcccaatac
      721 tgatcaagtg cacacctcac catcatcttt tagcaacgag tcaactttcc ttattcggct
      781 cttcctgttg tggaagcgca aaatttgatt gcgttgatct gccatttttc ctgtttatct
      841 ggccagcgtg cgttcggttt catttttcac cgttcctttt caggaaaaca aatcagatca
      901 gcagactctt gctccatgca cttgctccac ccggctacga ccaacgaaga cctcctccag
      961 ttgatgctga ccggagatga aaccgcgctt gagacactgt atgcacgcct gcacccggtt
     1021 atctaccggt ttgccttccg catgggaggg tcggctgccc tggccgatga cgtgtcacaa
     1081 gagactttcc tccagcttat ccaggaaggt gcgcgatttg atccgtcgcg cggaaacgca
     1141 acggcgtatc tgctcgggat tgcccggcat gtgctctgga atcgtctgga ccgggatcaa
     1201 aagtttgttc cgctggctga aaccagtgat gaatcagacc tttcgcccac cacatcaatt
     1261 gacctgacca cgccgctgac ttccgttttg cagcatgaaa ccgttgaaaa tgtccgcctg
     1321 gccattaccg gtttaccggc tcactaccgg gacgtcgtgg tgctgtgtga tttgcaggaa
     1381 ctcagttatc aggatgtcgc cacaatttta gggtgcccga ttggcaccgt tcgatcccgg
     1441 cttcatcggg cccacgacgt gttgtgtcag cggctgtttc agtttcgaaa tgcgagtttg
     1501 gatgtatcag tccagttgca tccggctgag cgaaatcatc gctgtgcctg atcaaaccca
     1561 ttgttctttc agaaaaaaag caacgctcat caccttttga acagaaggta atactcccac
     1621 tgaattccac gaaaaatatc tttcccaaca tcaggaaaac cggttttggg aaccactttt
     1681 gtacagattc ttttaaagga gacgccgcac atgaaagcca ccaaactgct tttgtttgtt
     1741 gcccttgtca gtttttttat cgccattcag ggaatgaaaa cacctgtgtt gaaagcccaa
     1801 accgatgatc cggctcttca aggaaccggc gttgaagcgc aaaccggttt tgaccacgaa
     1861 tcgaacggct ttttagatca aacttcattt gaagaagcca gagacggatt tgatgccgtc
     1921 gaagatctcg aagacggcgt gggccctgtg tttaatggaa aatcctgcgg tgaatgccat
     1981 gggaacccga tctcaggcgg gaatagccag gttgccgtga ttcgggcggg tcattttgat
     2041 ggcacgagct tcctggatca tcccggcggg tcgttgatcc atagccgggc cattgatccc
     2101 cgaatccagg aacggattat gccgggaaat gaaacccggt ccttccgcat gtcgctcagt
     2161 attttgggtg atggttttat cgaagccatt gacgacaatg cattgctggc cattgcccag
     2221 gctcaaaaaa ccgccaccaa tggaaaaatt caaggtcagg ccattctggt gccggtttta
     2281 gaagcaccag gggtttctcg tgttggtcgc tttgggtgga aagctcaaca tgccagcctg
     2341 ctctcgtttg ccggagatgc ctatttaaat gaaatgggaa ttaccacccc gatgttcccg
     2401 gttgaaaata cggccaatgg ccgggatgtc agccagtttg atacggtggc tgatcccgac
     2461 gaagctgaca acgacgacct cgaacttttt gccaacttta tgcgctcgac caaggccccg
     2521 tcacgcgacg aacgcatgat gaatgccccc gaaggacgca acggc
//
LOCUS       DAOQDO010000782         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35050_length_2564_cov_2.772818, whole genome shotgun sequence.
ACCESSION   DAOQDO010000782 DAOQDO010000000
VERSION     DAOQDO010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35050_length_2564_cov_2.772818"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKZ53_12720"
     CDS             <1..374
                     /locus_tag="PKZ53_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41349.1"
                     /translation="REALKSAQEQKNAPKEVKPEVADKPKSNIDPDIQKIEELSKPKS
                     DQTATTTTPSEPETDPVAEAERRLQRLLAQGQDKGFRETPPSPPQPKVEEVKKEEPKK
                     EEKKKEEKKIEETPVEEPISQ"
     gene            562..1941
                     /locus_tag="PKZ53_12725"
     CDS             562..1941
                     /locus_tag="PKZ53_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ41350.1"
                     /translation="MADILVVEDKDSLREMLCMTLERAGYSVAEAGDAARARRALQDQ
                     LPQLVLTDLRMPHGGGLEVLRATKALDPDLPVIILTAYGSIDEAVQAMKDGALDFLQK
                     PVDSEHLLLVVGRSIEAQRLQREAILLREEYSKRYGFPRIVGDSSVMQQVSRQIQQVA
                     VTNTTVLLLGESGTGKELFARAVHHLSPRANRPFIALNCAAIPETLIENELFGHEKGA
                     YTGADSRRAGKFELAQGGTIFLDEIGELPLAVQSKLLRVIEERTITRIGGSAEIVVDV
                     RIVTATNRDLNQAVADKTFREDLFFRLSIFPVRIPSLRERRSDIPQLAESFVQHYGRE
                     IRRSTIKISDEAMRSMVAYDWPGNVRELKSCIERACILCNGKVIEARDCSLFTPSQQK
                     SREDRFLEGLDFGGTLSEVTDRVTRMVEKRKIELTLEETSFNKTRAAELLGISLKTLA
                     SRMKELAIE"
     gene            complement(2119..>2564)
                     /locus_tag="PKZ53_12730"
     CDS             complement(2119..>2564)
                     /locus_tag="PKZ53_12730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNJ41351.1"
                     /translation="DAHPCQLPEKLMERVIQLTTNPGGLVFDPFGGAGTTALAARKLG
                     RDFVMLDLDPNYVELARRNLALMEAQAQTAGVLEVPRARVAKPKSNGSKKEVEMLLQS
                     LARQLGRVPLEEDIAAHHPTLLETIDGLYPNRSAAIKRSRVALVE"
BASE COUNT          656 a          686 c          663 g          559 t
ORIGIN      
        1 cgcgggaggc attgaaatca gcccaggagc agaaaaatgc ccccaaagaa gtgaaaccgg
       61 aagtcgcgga taagccgaaa tccaacattg atcctgatat tcagaaaatc gaagaacttt
      121 ccaaacccaa atcagatcag acagccacca cgaccacacc atcggaacca gaaacagacc
      181 cggttgctga ggctgaaaga cgtcttcaac ggttgctggc ccaaggccag gataaagggt
      241 ttcgtgaaac cccaccatcg ccacctcaac ccaaagtaga agaagtcaaa aaagaagagc
      301 cgaaaaaaga ggaaaagaaa aaagaggaga agaaaataga agaaacacct gtcgaggaac
      361 caatcagtca gtaactccaa ttgggtgtga ggttcgatca ttccaatgta tccgaagaat
      421 gagcttgtgg ggtatttcgg aaaggtgttt cattgaaaaa attatgccgt cccccaatct
      481 tcagaaatga gtgaacctct cgcttcataa ccattacctg ccagactttc caaaacttcc
      541 gactccccaa ttccagctta tatggctgat attctggttg ttgaagacaa agattccctc
      601 cgtgaaatgc tctgtatgac gctcgaacgg gccgggtaca gcgttgccga agctggcgac
      661 gccgcccggg cccggcgggc gttacaagat cagctcccac aactggtcct gaccgacctg
      721 cgcatgccgc acggcggagg gttggaagtc ctgcgcgcca ccaaagcgct tgatccggac
      781 ctgccagtga tcattttgac cgcgtatggc tcgattgatg aagcggttca ggcgatgaaa
      841 gatggcgccc tcgatttcct gcaaaaaccg gttgactccg aacatttgct gctggtggtt
      901 ggccgctcga ttgaagccca gcgattgcaa cgcgaagcca tcttactgcg cgaggaatat
      961 tcaaaacgct atggctttcc acgcattgtg ggtgattcgt cagtgatgca gcaggtcagc
     1021 cgacaaatcc agcaagtcgc cgttaccaat accaccgtgc tcctgttggg cgaatcaggc
     1081 accggcaagg aactctttgc tcgtgccgtt catcacctca gcccacgtgc caaccggccc
     1141 tttattgccc tcaactgtgc cgcaattcct gaaacactga tcgaaaacga actctttggc
     1201 cacgaaaaag gggcctacac tggggcggat agccgccggg ctgggaaatt tgaactggcg
     1261 cagggcggca cgatttttct ggatgaaatc ggtgaattgc cactggccgt tcagtccaaa
     1321 ctgctgcgag tgattgagga gcgcaccatt acccgtattg gcggaagtgc cgaaattgtg
     1381 gttgatgtgc gcatcgtgac cgcgaccaat cgtgacctga atcaggcagt ggcggacaaa
     1441 acctttcgtg aagatttgtt ctttcgactc tcgatttttc cggtgcggat tccttccctg
     1501 cgggaacgcc ggtctgatat cccacaactg gctgaaagct ttgtccagca ctatggtcgt
     1561 gaaattcgcc gtagcaccat caaaatttcg gacgaggcca tgcgttcgat ggtcgcctat
     1621 gactggccgg gcaatgtccg cgaactcaaa agctgtatcg aacgcgcctg cattttgtgc
     1681 aatggaaaag tgatcgaagc cagagattgt tcgctcttta ctccgtctca gcaaaagagc
     1741 cgtgaagatc gctttttgga aggacttgat tttggcggga cgctttccga agtcactgac
     1801 cgggttaccc ggatggttga aaaacgcaaa attgaactga cgctcgaaga aaccagcttt
     1861 aataaaaccc gcgcggctga actgctcggc atcagcttga aaaccctggc gtcacggatg
     1921 aaagaactgg cgattgagta atctttgagg gctgagggcc tggggctaag ggctgaaatc
     1981 tttcacatgt ggttttttgg gtttgcacct cgaagagtga caattcaaaa tgctcaatct
     2041 acgtctcaac ccgtctcaga ccaatgagag gcaatcaaaa cctacctgag aaagagcccc
     2101 acctcactca atttgtcttt actcaaccag ggccacccga ctgcgtttaa tggctgccga
     2161 acggtttgga tacagcccgt caatcgtttc aagcagcgtc ggatgatggg ccgcaatgtc
     2221 ttcttcaagc ggaacccgcc caagctgacg cgccagtgat tgcaacagca tctcaacttc
     2281 tttctttgag ccattcgact ttggcttggc gacgcgagcg cgtgggactt caagcacacc
     2341 agccgtttgg gcctgggctt ccatcaatgc cagattgcgc cgggccagtt cgacatagtt
     2401 tggatccaga tcaagcatta caaaatctcg tcccagcttt cgggcagcca gcgccgtcgt
     2461 cccggccccg ccaaatggat caaacaccag cccgccgggg ttggttgtca attggatgac
     2521 tcgctccata agtttttccg ggagctggca ggggtgcgca tccc
//
LOCUS       DAOQDO010000783         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35222_length_2558_cov_2.889732, whole genome shotgun sequence.
ACCESSION   DAOQDO010000783 DAOQDO010000000
VERSION     DAOQDO010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35222_length_2558_cov_2.889732"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1244
                     /locus_tag="PKZ53_12735"
     CDS             96..1244
                     /locus_tag="PKZ53_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ41352.1"
                     /translation="MINRERMLNHVLELIQIDSHSREERDVAMKLKADCEALGGTVYI
                     DHAGEKVGGNTGNVIVHFKGTNPNAPPFFLSSHMDTVVPGKGVKPIREGNIIRTDGTT
                     VLGGDDKTGCSIIVECIRTLQEQNIPYSDLQIVFTICEEVGLLGAKNCEIDRVSAKFG
                     LVLDSDDPGHLITKAPSSNRMRFTIYGLEAHAGVCPEKGISAIKIASEAIAEMKLGRI
                     DHETTSNIGTINGGVAVNVVPNRVDLYCEARSHNDDKLETQTSHMVDCLERAVSHHEV
                     TNDGHTVKARLEKHIERDYHRMDLADDSMIVRLVKQAAHNLGHDCEPLPTGGGCDANI
                     FNRKGIECANLGTGMHNIHTVKEYMSIDDFYQSADIVFEVLKLHGQNN"
     gene            1376..2377
                     /locus_tag="PKZ53_12740"
     CDS             1376..2377
                     /locus_tag="PKZ53_12740"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNJ41353.1"
                     /translation="MIIEAKAPTRIDLAGGTVDLWPLYLLHQEAQTINVAIDLLATCR
                     VEERLDEQIILVSEDINLSETYESLGEIRLDTPLQLLARMVDYFKPYTGVKVTTRCAA
                     PPGSGLGGSSALAVALAGALDHLTGNRFSDSQLLKIAQNIETQVIEVPSGVQDYYPAM
                     YGGTNSLHLEVIGVVREALSIDLAKLQDHVVLCYTGKPHFSGTNNWEIFKAHIDNRDG
                     VRERFASLCEVTNRMRIAVLEQNLPGIATCLTEEWELRKGFSEHVTTDTIEQLISVAK
                     EQGALAAKVCGAGGGGCVVFLVETGRKADVSRALTEAGGEILEVNLIDRGLRVEQVG"
BASE COUNT          675 a          636 c          636 g          611 t
ORIGIN      
        1 accctgaacc ctgaaccctg aaccctgaac cctgaaccct gaaccctgaa cccggcatca
       61 ctcaccttca cctctttaaa ggaattcctc ctcatatgat caatcgcgaa cgcatgctca
      121 atcacgtttt ggaactcatc caaattgata gtcactcgcg tgaagaacgg gatgtggcaa
      181 tgaaactcaa ggctgactgt gaagctttgg gaggaacggt ctacattgac catgccggtg
      241 aaaaagttgg tggaaatact ggcaatgtca ttgtccattt caaaggaacg aacccgaatg
      301 ccccgccatt ttttctgtcc tcacatatgg ataccgtcgt gccaggaaaa ggggtgaagc
      361 caattcgaga aggcaacatc attcgcaccg atggaacgac tgtccttggt ggcgacgaca
      421 aaactggatg ctcaattatt gtggaatgta tccgcacgct tcaggagcag aatattccgt
      481 acagcgatct tcagattgtc tttaccattt gcgaagaagt cgggttactg ggcgcgaaaa
      541 actgcgaaat tgaccgggtc agcgcaaaat ttggactggt gcttgatagt gatgatccgg
      601 gtcatttgat taccaaagcc ccatcgtcaa accgaatgcg gtttaccatt tacggactcg
      661 aagcccatgc gggtgtctgc cctgaaaaag gaatctcagc catcaagatt gcctctgaag
      721 cgattgctga aatgaagctg gggcgcattg accatgaaac aacttccaac atcggcacga
      781 tcaatggtgg agtggcagtc aatgtcgtcc ccaaccgggt ggatctgtac tgtgaagcgc
      841 gcagtcacaa tgacgacaag ctcgaaaccc aaacttctca catggttgac tgcctggaac
      901 gagcggtttc ccatcatgaa gtgaccaatg atggacacac cgtcaaagcc cggcttgaga
      961 aacacattga gcgcgactat caccgcatgg atttggccga tgattcgatg attgtcagac
     1021 tggtcaaaca agcagcccac aatcttgggc acgattgcga accattgccg actggcggtg
     1081 gatgtgatgc aaatatcttt aaccgtaagg gaatcgaatg cgccaatctt ggcaccggaa
     1141 tgcataatat ccacacggtc aaagaataca tgtcaattga tgacttctat caatcagcgg
     1201 acattgtgtt tgaagtcctc aaactccacg ggcagaacaa ctgatctggt atctggtacc
     1261 tggtgcttgg tttttgattt ttggttcagg gttcttgtaa agtatttttt tctaagcact
     1321 aaacaccaag cactaaacac caagcactaa acaccaagca ccaagccctc atcccatgat
     1381 tattgaagca aaagctccga cgcgaattga cctggcaggt ggaacggttg atctctggcc
     1441 actctatctg ttgcatcagg aagcccaaac tatcaacgtg gcaattgacc ttctggcaac
     1501 ctgccgggtt gaagaacgac ttgatgaaca gattattttg gtttcggaag atatcaatct
     1561 aagtgaaacc tatgaatcac ttggggaaat tcggcttgat accccactcc aactccttgc
     1621 gcggatggtt gattacttta agccatatac tggggtaaaa gtgaccactc gatgcgcggc
     1681 accacccggc tctggtctgg gtggctcttc agcgctggcg gtggcactgg caggcgccct
     1741 ggatcatctc accgggaacc gatttagtga cagccagctt ttgaaaattg cccaaaatat
     1801 cgagacccag gtcattgagg tgccgtcggg tgtgcaggat tactatccgg cgatgtatgg
     1861 cggaacgaac agtttacacc tggaagtgat tggggttgtc cgcgaggcac tctccattga
     1921 tctggcaaaa cttcaagatc atgtggtgct gtgctacacc ggaaaacctc attttagcgg
     1981 tacaaacaac tgggaaattt tcaaagctca tattgacaat cgagatggcg tccgcgaacg
     2041 atttgccagc ctgtgtgaag tcaccaatcg catgcgcatt gccgtgctcg aacaaaattt
     2101 gccaggtatt gccacctgcc tgactgaaga atgggaactc cgcaaaggat tttcagaaca
     2161 cgtcaccact gacaccattg aacaattgat ttctgtggct aaagaacagg gtgccctggc
     2221 cgccaaagtc tgtggggctg gtggcggcgg atgtgtggtg tttttggttg aaactggccg
     2281 caaagccgat gtcagtcgag ccttgactga agccggagga gaaatcctgg aagttaattt
     2341 gattgatcgt ggcctgcgag tcgaacaggt aggataaaac cagggccagg ggctcagggc
     2401 taggggctga agaaaacggg ctcagggtgt tgaacccgtc ttcttcagcc cttaaccttc
     2461 agccccaagc ccaatgtctt cagccctact tcttcagccc taaactccta tgattcgtac
     2521 ctggctttcc cgaatctggt cttctccctc ggcaatgg
//
LOCUS       DAOQDO010000784         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35249_length_2557_cov_2.497202, whole genome shotgun sequence.
ACCESSION   DAOQDO010000784 DAOQDO010000000
VERSION     DAOQDO010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35249_length_2557_cov_2.497202"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PKZ53_12745"
     CDS             complement(<1..1635)
                     /locus_tag="PKZ53_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41354.1"
                     /translation="MIPSRRLSLFIFLLSVVFLYFPRYLGEVSAQKQSPSPTAFPSPG
                     QSSISIQAAGRGTPWVNFQDGYALPSFYPPSDSPTKAPTAFQPTTLTTADFDEDGVPD
                     LVTGMRSTSGGRLILCRGNVDSIFPNSPDAQRRRATGTLIESPFLPPVQEVTVLDAPD
                     FVGGGDFDADGHADLVVLTRGKSELLLISGDGQGGLHPTQRIDLPGLVTTAAVSQLSL
                     ATGSASLAIALQGSDGDELLFLSHSQQVGMSDFQRMKLPGTVTACTFGQLTQTSTADL
                     IVAVGNQLVLFQEKSLLAGNKFNADQVQSFPFVTKAIATGQFYHNQPPQLALLTDQGH
                     LMVGAIGSSDFSSRGQWTGATSLVTARVSTTGSDDLVVLDQQNQLHIVLDQESESVTK
                     RTISPVAPVTFDTQRAPVAVLPMRLNIDGLNDLVILHAGDPQPAVTLTAPVHTFVVSS
                     TDASSSSGDMNPGDGICRDFNGNCTLGAAIGEANASAGADLITFNIAPGGPQTIQGDV
                     FFGAIFNQTYDFSETATLDATTQPGFAGTPIISFTGL"
     gene            2228..>2557
                     /locus_tag="PKZ53_12750"
     CDS             2228..>2557
                     /locus_tag="PKZ53_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013796716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNJ41355.1"
                     /translation="MLERLFQLQERNTTVRTELIGGVTTFVTMAYIIVINPAILSFAG
                     LPIGPSTVATILAAVFGCALMGLYANRPIAVAPYMGENAFIAFGLVALGITWQQRLGA
                     VFISGIGF"
BASE COUNT          649 a          658 c          660 g          590 t
ORIGIN      
        1 cagaccagtg aaagaaataa tcggagtccc cgcaaatcca ggttgtgtcg tggcatcaag
       61 cgttgcggtt tctgaaaaat cgtacgtttg attaaaaata gccccgaaaa agacatcgcc
      121 ctggatggtt tgtgggccgc caggcgcaat gttaaaggta atcaaatcgg cgccggcact
      181 ggcattggct tcgccaatcg cggcaccaag cgtgcaattg ccattaaaat cccgacagat
      241 tccatctcca ggattcatgt ctccagaact tgacgaggca tcggtactgc tgacaacaaa
      301 ggtgtgaact ggggcggtta acgtaaccgc tggctgggga tctccggcgt ggagaatcac
      361 cagatcgttc aacccatcaa tgttgagccg catcggtagc acggccaccg gggcacgttg
      421 ggtgtcaaag gtcaccggag caaccgggct gattgtccgt ttggtcactg actcactttc
      481 ctgatccaaa acaatgtgga gctgattctg ttgatccaaa accaccaggt catctgaccc
      541 cgtcgttgac acccgcgcgg tcaccagcga tgtcgctccg gtccattgcc cgcgtgacga
      601 gaaatcactg gacccaatgg ctccaaccat caggtgcccc tgatcagtca aaagtgccag
      661 ttgtggtggc tggttgtgat aaaactgccc ggttgcaatg gctttggtca caaatggaaa
      721 tgattggacc tggtcggcgt tgaacttatt cccagcaagt aagctctttt cctggaacaa
      781 taccaactgg tttccaaccg ccacgatcaa gtcagccgtt gaggtttgcg tgagctggcc
      841 aaacgtacag gccgtcaccg ttcctggaag tttcatccgt tgaaaatcag acatcccaac
      901 ttgttgcgaa tgactcagaa acagaagctc atctccatcg ctcccctgga gagcaatcgc
      961 caaactggct gacccggtcg ctaaactgag ctggctcacg gcagcggttg tgaccagccc
     1021 tggcaggtca atccgctggg ttgggtggag cccgccctga ccatcaccag agatcaaaag
     1081 caactcgctt ttcccacggg tcaggaccac caggtcagcg tggccatcag cgtcaaagtc
     1141 tccgccacca acaaaatcag gcgcatccag tacggtcact tcctggactg gcggcaaaaa
     1201 cggagactca atcaatgtac cagtggctct tcgacgctgg gcgtccgggc tgtttggaaa
     1261 aatcgaatcc acattccccc ggcacaaaat caggcggcca ccactcgtgg aacgcattcc
     1321 ggtgactaaa tcaggcacac cgtcttcatc aaaatcagcg gtggttaacg tggtgggctg
     1381 aaatgcagtt ggagctttcg ttggtgagtc tgatggcggg taaaaggacg gtaacgcata
     1441 tccatcctgg aaattgaccc agggcgttcc ccgccctgca gcctgaatcg agattgatga
     1501 ttgaccaggg ctgggaaagg cagtcgggga tggtgattgc ttctgggcgc ttacttcacc
     1561 cagatacctc ggaaagtaga gaaaaacaac gctcaataaa aagataaaaa gtgagagacg
     1621 gcgtgacgga atcatttgaa tatactcctg aatacaaatt gattggccca caatttttct
     1681 gtatgggaaa gagggaggca gaaaagttta ggagtcaaaa tgcgaaatca gattggaagc
     1741 gctgggaatt cagcatcgtc aatatcacaa ggaatcaacc tgcgcttccc aggtcatatg
     1801 gtgggctcaa cctgaacgcg caaaaacaag caacgtgctc tcatgtggaa atagagaaaa
     1861 cccaccttga ctacacaaaa ctccagttcc aaacactcct ggcaactttc aggaccaggg
     1921 ttgaaacagg caatggcttt cgtcatcttt gaagtgagga gaagcactgc cgaaaatcgc
     1981 tgtcttccag gatacccaac aactttttct tacctgggta ctaattgtta ccagagacgt
     2041 gattcaaggt gggtgagata ggtcaaacag taccgttggg tgcgctcagc aatcctcaag
     2101 aaaagctcaa ttttttatca gttttgagtt ttcgaaacat ggctcctcca gttgcggaat
     2161 ctgcgggttg agataaactc gcagccgctc gtccctcatg actttcactc aaggatcatt
     2221 cacttcaatg cttgaacgac tgtttcaact tcaagagcgg aataccacgg ttcggacaga
     2281 gttgattggc ggtgtcacca cgtttgtcac catggcctat attatcgtca tcaacccggc
     2341 aattctgagt tttgcgggtc ttccgattgg ccccagcacg gttgccacga ttttggcggc
     2401 tgttttcgga tgtgccttga tggggctcta tgccaatcgc ccgattgccg tggcgcccta
     2461 catgggtgaa aacgccttta ttgcctttgg gttagtggct cttggcatta cctggcaaca
     2521 gcgtctggga gcggtattta tcagcggcat tggattt
//
LOCUS       DAOQDO010000785         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35314_length_2554_cov_3.687875, whole genome shotgun sequence.
ACCESSION   DAOQDO010000785 DAOQDO010000000
VERSION     DAOQDO010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35314_length_2554_cov_3.687875"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKZ53_12755"
     CDS             <1..718
                     /locus_tag="PKZ53_12755"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ41356.1"
                     /translation="HWFDGVQWQDFASFGSGLNQVRAPQGVTLDATGKFLFVADTSNN
                     RILRFDALSPFSNATVIATAGTGAGQVRAPQGLAIDLNNTLYVADTGNNRVVKFANAT
                     TTPVFSVVAISGTTLNRVRGPQGLAVDNELNLYIADTANSRVLFFPLGTTAGAQAIST
                     VGSGPGQVRLPEGITVVTGPEGKRLVISDTGNNRIQSTSLVSTAPFTPGDWRLVAGPG
                     SQTGAIGSLIGQFRTPGKIR"
     gene            1023..2168
                     /locus_tag="PKZ53_12760"
     CDS             1023..2168
                     /locus_tag="PKZ53_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ41357.1"
                     /translation="MNFDLTEEQLQVKYSVREFAEAEIKPHIMEWDEEQVFPLELQPK
                     LADLGLLGIIIPEEFGGAGMGYVEYATILEELARVDPSITLSVAAHNSLCTNHIYQMG
                     NKQQREEFVPPLARGEQMGAWALTEPGSGSDAAGMRTMAIRENGSWVINGSKNFITNA
                     SHAAVTVVMAITDKSKGNHGVSAYIVKNDTPGFTVGKKENKLGMRASNTASLNFDDCH
                     VPVESMVGEYNHGFVDSLKILDGGRISIAALSLGIAQGAYESALKYAKQRQAFGRVIA
                     EYQAIQWKLADMATQIEAARLLLYQAAVLKDRGKVTTRQSAMAKLYASEVAVKVSEEA
                     IQIHGGYGYTKDYPPEKFWRDSKLCTIGEGTSEVQRLVIAREILRAS"
     gene            2176..>2554
                     /locus_tag="PKZ53_12765"
     CDS             2176..>2554
                     /locus_tag="PKZ53_12765"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNJ41358.1"
                     /translation="MRRLDAALVFKSRRTLDEIPQVVLSQLPLHKRLIYLALPLGWMV
                     CLFFFSTDVLSSNQTGSVIEPILHKVVHTIDPTASEKAVWVGHLLLRKLGHLSEYACL
                     AVLWAIGLRKTSGWSRRRVLMMAL"
BASE COUNT          612 a          660 c          642 g          640 t
ORIGIN      
        1 ccactggttt gatggagttc agtggcagga ttttgccagc tttggttctg ggttgaatca
       61 ggtgcgtgcc ccacaggggg taacccttga tgcaactgga aaattcctgt ttgtggctga
      121 taccagcaac aaccgaatcc tgcgatttga cgcattgtcg ccattttcaa atgccaccgt
      181 gatagcaacc gctggcaccg gtgccgggca ggtccgagca ccacaaggac tggcaattga
      241 tcttaacaac acgctgtatg tggcggatac cggcaacaat cgggtggtga agtttgccaa
      301 tgccaccacg acaccggttt tcagtgtggt cgccatctct ggaacaacac tcaatcgagt
      361 tcgtggacca cagggactgg cagttgataa tgaactgaac ctctatatcg ccgacacagc
      421 caatagccgg gtgttgttct tcccacttgg aaccactgct ggtgcccagg ccatttccac
      481 cgttggctct ggtccgggac aggttcgatt gccagaaggc atcaccgttg tgactggacc
      541 tgaaggaaaa cgactggtta tcagcgatac cggcaataat cgaatccaga gtacatcact
      601 tgtcagtacc gctccgttca caccgggtga ctggcgactg gtggctggcc ccgggagcca
      661 aaccggcgcc atcggctcgt tgattgggca attcagaacc ccaggcaaaa tcagataaga
      721 cgatttccag atttttgaaa aaggcgcggc tgctttgagc tgcgccttta ctctttaata
      781 cctctgtcac tttgtcattt tgtcacctgc cacctgtcat ctccaccgct ttttttcctc
      841 cctccattgc caattcctga ctaactcata taatcggggc acgtctttat tttctttttc
      901 ctcagcacct ttcatcgcat tttttcttca gcctgcactc tggcatctct caagattgac
      961 cagatcaaat cgaaggtgcc gtcttctttt ttcgttcaaa cgagggccct gatatgtcaa
     1021 ttatgaattt tgatctaacc gaagaacagc ttcaggtaaa gtattctgtt cgtgagtttg
     1081 ctgaagctga aatcaagccg cacattatgg agtgggatga agagcaagtc ttcccacttg
     1141 agttacaacc caaactggcc gacctgggct tgctcggcat tatcattcct gaggagtttg
     1201 gtggcgccgg aatgggctat gtcgaatatg ccaccattct cgaagagctt gctcgcgttg
     1261 atccgtcaat caccttgagt gtagctgccc ataactcatt gtgcaccaat cacatttacc
     1321 agatgggtaa caagcaacag cgcgaagaat ttgtcccacc actcgcccgt ggcgaacaaa
     1381 tgggcgcctg ggcgctcacc gaacctggct ctggaagcga tgccgcagga atgcgcacca
     1441 tggcgatccg ggaaaatggt tcgtgggtga ttaatggcag caaaaatttt attaccaatg
     1501 cgtcacacgc tgcagtaaca gtcgtgatgg ccattaccga taaatccaag ggcaatcatg
     1561 gagtttcggc ttacattgtg aaaaatgaca cgccgggatt tacggtggga aagaaagaaa
     1621 acaaacttgg aatgcgtgcg tcaaatacgg ccagtcttaa ctttgacgac tgtcatgttc
     1681 cagttgaaag catggtcggt gaatacaacc atggctttgt ggacagcttg aaaattctgg
     1741 atggcggacg catttcgatt gcggcgctgt cactgggcat tgcacagggc gcctatgaat
     1801 cagctctgaa atatgccaaa caacgtcagg cgtttggccg cgtcattgcg gaatatcaag
     1861 cgattcaatg gaaactggcg gatatggcca cccaaattga agccgcccga ctgctgttgt
     1921 atcaagccgc cgtgctgaaa gatcggggta aagtcaccac ccgtcagtct gcgatggcga
     1981 aattgtacgc ctcagaagta gccgtgaagg tttcagaaga agccatccag atccatggcg
     2041 gatacggcta taccaaggac tatccacccg aaaaattctg gcgtgattcc aaactctgca
     2101 ccattggcga aggaacctcc gaagtccaac gcctggtgat tgctcgcgaa attctgcgcg
     2161 ccagctaaat gtggagtgcg gcggcttgac gccgctttgg tttttaaaag ccgccgcact
     2221 ctggatgaaa tcccgcaggt cgtcttgtcc caactcccgc tccacaaacg attgatctat
     2281 ctggcacttc cacttggatg gatggtctgc ctgtttttct tctcaaccga tgtcctgtcg
     2341 tccaatcaaa ctggctcggt gatcgaaccc attctgcata aagttgttca caccattgat
     2401 ccaaccgctt ctgaaaaagc agtctgggta ggacacctgc tgcttcgcaa acttggtcat
     2461 ctctcagaat atgcgtgcct ggcagtattg tgggctattg gattgaggaa aacgagtggg
     2521 tggtcgcgtc gccgtgtctt gatgatggca ctga
//
LOCUS       DAOQDO010000786         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35387_length_2551_cov_3.995192, whole genome shotgun sequence.
ACCESSION   DAOQDO010000786 DAOQDO010000000
VERSION     DAOQDO010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35387_length_2551_cov_3.995192"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..2113)
                     /locus_tag="PKZ53_12770"
     CDS             complement(77..2113)
                     /locus_tag="PKZ53_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNJ41359.1"
                     /translation="MTVDNLITIRHLERRALIYVRQSTPQQVLTHQESLRLQYALHQR
                     AQELGWPASRIEIIDTDLGLSAAAASHRSGFQALVAQVTLGQVGIILSIDVTRLSRNC
                     SDWYPLLDLCGFKQCLIGDRDGVYDPGTTNGRLLLGLKGQISELELRTIRLRLTTGLL
                     QKAQRGELVQKLPVGLERDELNRVMKDPNLEVQHRIELVFEIFLEVGAACRTVKQLQA
                     LGLALPRRNAAGDVVWKRATVEAVLAILHNPAYAGVFVYGRSRQVRPDLSRRPWHRPL
                     PISEWNIQVAGKYPAYIDWSTYERIQQMLTENRAEYSRRATPGIARNGQALLQGVAWC
                     GECGHKLCLTYRPIPHYCCVALHHRYGDPMCQRIAAQVVDGSVVQAFFEALSPAELNL
                     YEEVMAGERVSAEQAAQAQAQQLQRLEYQARLAERQFCRVDPENRLVAAELERRWETA
                     LRAVKAAGALTEPTPVQDQPLTPDLKAALLDLGQKLPELWNTGWLTLAQKKKLIRCLI
                     EKVVLQRQPRDRVQVRLVWRGGAVTEWNLPLPVGSFSDLPNASQLEQRVLELVRTGAS
                     DQAIAHQLTGEGFRSPRHSILLESTVRRLRLQHRLLINRHHPQPGRPANSWSVSDVTT
                     QLRLPPHWIYYQIQCGRLQIPRDPDTGLYFLPAHSSTLEQLEQLKNGFVQTVYF"
BASE COUNT          602 a          708 c          665 g          576 t
ORIGIN      
        1 agacggcccg gttcttgatg attttgcgca aactgtaatt gagcgattca attgagtcgc
       61 catgatggga cacctcctaa aaataaactg tttgaacgaa gccgtttttt aattgctcca
      121 attgctccaa agtggatgaa tgagccggca aaaaatacaa cccggtatct ggatcacgtg
      181 ggatttggag acgcccacac tgaatttgat agtaaatcca atgaggtggg agtcggagtt
      241 gggtggtcac gtcactgacc gaccaggaat tggctgggcg accaggctga ggatgatggc
      301 gattgatgag aagccggtgt tggagtcgga ggcgtcggac ggtactttcc agcaggatcg
      361 agtgacgtgg cgagcgaaag ccctcaccag tcaactgatg ggcaatggct tgatccgaag
      421 ctccagtccg caccaattca aggactcgct gttccaactg tgaagcattg ggtaaatctg
      481 aaaacgatcc gaccggaagc gggagattcc attctgtcac ggcgcctcca cgccagacaa
      541 gacgcacctg aacccgatcc cgaggctgac gctggagcac aactttctca atcaaacagc
      601 gaatcaactt ctttttttga gccagggtaa gccaacccgt gttccaaagc tcaggcaatt
      661 tttgtcccaa gtcaagcaag gctgctttca ggtcgggagt caggggttga tcctggacgg
      721 gagtcggctc agtgagtgct ccagccgctt ttacagctcg gagtgctgtt tcccatctcc
      781 gttccagttc agcggcaacg aggcggttct ccgggtcaac ccgacaaaac tgccgctctg
      841 ccagtcgggc ctggtactca agccgctgaa gttgttgcgc ctgggcctgg gctgcttgtt
      901 cggcactcac ccgctcgccg gccatgactt cttcataaag attgagttca gcaggggata
      961 atgcttcaaa aaacgcctgc accaccgacc catcgacaac ttgagctgca atgcgctggc
     1021 acatcggatc cccataacga tggtgcaggg ccacgcaaca ataatgagga atggggcgat
     1081 acgtcaggca gagtttgtga ccacattccc cgcaccaggc tactccctgg aggagcgcct
     1141 gcccattgcg ggctatccct ggcgttgcgc gacgggaata ctcggcacgg ttttcggtta
     1201 acatttgttg aatccgttca taggtagacc agtcaatata agccggatat ttgcccgcaa
     1261 cctgaatgtt ccattcggag atcgggagtg gacgatgcca gggccgccgg ctcaagtccg
     1321 gtctaacttg ccgactccgt ccatagacaa agacaccggc ataagccgga ttatgcagta
     1381 ttgccagcac cgcttccact gtcgctcgct tccaaactac atccccagcc gcatttcgcc
     1441 ggggaagcgc caatcccaaa gcctgcaact gcttaaccgt tcggcaagcg gctccaacct
     1501 ccaaaaagat ttcaaacacc agctcgattc ggtgttgcac ctccagattg gggtccttca
     1561 tcacccggtt gagttcatcg cgttccaaac caaccgggag tttttgcact aactccccac
     1621 gttgcgcttt ctgaagcaac ccggtggtga gccgtaaccg aatcgtgcgc aactccagct
     1681 ctgaaatttg tcccttcaat cccaacagca gccgaccatt ggtcgttccc ggatcataca
     1741 ctccatcccg gtcgccgatc aaacattgct taaacccgca caggtccaag agtggatacc
     1801 aatccgagca gttgcgtgac aaccgcgtga catctatcga caggataatt ccaacctgac
     1861 ccagggtcac ctgcgccact aacgcctgga atccagaccg atggctggct gcggcagcgc
     1921 ttaaccccaa gtccgtatca ataatttcaa tccgggaggc gggccatccc agttcctggg
     1981 ctcgttggtg cagtgcatac tgcaaccgga ggctctcctg gtgggtgagc acttgctgtg
     2041 gggttgattg ccggacataa attaatgccc ggcgttccaa atggcggatg gtaatcagat
     2101 tgtcgactgt catgcactac ctccttcaag actcggatga gttcaactcg gatggattga
     2161 cgctgctctt cggggagact ggcccacacg atgcctgggg taatcgacat attccctcgc
     2221 tttctctcgc caggtgaatc agatccatta tcccttcagg tgtgaactta gttctgactt
     2281 ttggacgaga aacccccaga ttggtcgctg ccagcacgat ccgcaagcat ctggattccc
     2341 gatacgcaac ccagacatga ccgtcctcaa tgagtgatga agtttggttc agcaacggaa
     2401 aacgccgacc ataaagagga tgggttgggt cggttacttc aatttcaggg gagggttcaa
     2461 agacttcttt tttaccagga gtagcttgtg tgggattcaa ttgaatcgct caattacagt
     2521 ttgcgcaaaa tcatcaagaa ccgggccgtc t
//
LOCUS       DAOQDO010000787         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35407_length_2551_cov_2.181891, whole genome shotgun sequence.
ACCESSION   DAOQDO010000787 DAOQDO010000000
VERSION     DAOQDO010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35407_length_2551_cov_2.181891"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..788)
                     /locus_tag="PKZ53_12775"
     CDS             complement(207..788)
                     /locus_tag="PKZ53_12775"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNJ41360.1"
                     /translation="MKIVGIPRALYRVLIVGLILCGLTGSASAQVDPETLVGLRSVWS
                     VDKARPGDVIHLAVQVTIQDGYHINAHQLKDEFLIPTELKITPSKGLQAANPIYPEAI
                     SHQVSFSETPLSVYEGNVHILVPVTIGRKLRPGKYTVTGSFRYQTCNDESCFPPHTVK
                     LQIPLEIVRTGTVVQPQHPELFPQAAPSKAIGH"
     gene            complement(809..>2551)
                     /gene="recJ"
                     /locus_tag="PKZ53_12780"
     CDS             complement(809..>2551)
                     /gene="recJ"
                     /locus_tag="PKZ53_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNJ41361.1"
                     /translation="DSSLSLTHSSWKLLEVDQATIRLLIQELSIPPLLAQCLANRGIS
                     TPTQAERFLNPSLSDIPDPRGMRNLQEAVQVVSGALARREKVRIVGDYDCDGTTGLIT
                     LLNVFRILSPAADEFLSFHVPDRERDGYGLNAGIVERAATDDVRVLISVDIGITAYKE
                     WAMVRERGMTGICLDHHTVLGSSVPPDAIIVCPKQSGCDYSEKDLAACGLSLQFGRAL
                     LADHPKRDRIVESLTKLVAIGTVADLVPLSSPSNRAIVANGLKGLSSGSSNPGLAALL
                     EVARLSGKQIAASDLGFRLGPRINAAGRIDGSTLSVVDLFDSKSLDEAKRRAAQIDTW
                     NSERQDIQRNLVDMLHQEIGNQDPNDLVFVLAGEHQAGWHQGVVGIAASKVVEQYHRP
                     ALVCSIRGSHAHGSARSIPQFNMVDALQHIHDGLFHRYGGHAAAAGFSLPAEAIPELR
                     SRINEYARSILSQEDLIQTRRYDGTTSLGQITPQLIESLARLAPYGINNPAPRLVVTA
                     TVTDRKILREHHLKLWLIDGNVRMEAIWWQRADLSDQIPVGVPIQVLGRIELNEWNGM
                     SRPQMIIEDIRLAS"
BASE COUNT          585 a          663 c          700 g          603 t
ORIGIN      
        1 agcctgtcaa atgggggatg caagaccatt gatttttcag aacagacagt cagtatgctt
       61 ccaagtggcg acaaagcaca ctgccaccaa acggctcatt aacacgctcg tttcgttttt
      121 ggttactgca gtaaaggttt ttagtcagga tatttagcgg tagaattcag atttgtaaga
      181 gatcgcaatc gggcattgag ttttcctcaa tgcccgattg cttttgaggg ggcagcttgg
      241 gggaagagtt cagggtgttg cggttgaacg actgtgccgg ttcgaacaat ttcaagtgga
      301 atttgaagct tgaccgtgtg tggcgggaaa caggattcat cattgcaggt ttggtatcgg
      361 aatgagccgg tcaccgtgta ttttcctgga cgcagttttc ggccaatcgt aactgggaca
      421 agaatatgga cgttcccttc atagaccgat aagggagttt cagaaaacga aacctggtgt
      481 gagattgctt ccggatagat tgggttcgct gcttgaagcc ctttggatgg agtgattttc
      541 agttcagtcg gaatcagaaa ctcatctttg agttgatggg cgttgatatg atagccatcc
      601 tgaatcgtca cctgtacggc caggtggatc acatctccag ggcgggcctt atccactgac
      661 cacaccgacc gaagccccac cagcgtttcc gggtcaacct gggctgacgc tgaaccggtc
      721 aatccgcaca ggatcaatcc cacgatcaac acccggtaca gggcacgtgg tattccaact
      781 atcttcattg aaaagccttc tttctgtgtc aactggccag ccggatgtct tcaatgatca
      841 tctgtggtcg gctcattccg ttccattcat tgagttcgat gcgccccagg acctgaattg
      901 gaactccaac tgggatttgg tcagataaat cagcccgttg ccaccagatt gcttccatgc
      961 gcacatttcc atcaatcagc cagagtttta aatggtgttc gcgcaggatt ttgcggtctg
     1021 ttaccgttgc ggtgacgacc agtcgtggcg ctggattatt gatcccatat ggtgccagtc
     1081 gggccagaga ttcgatcaac tggggtgtaa tctggccaag gctggtggtc ccatcataac
     1141 gacgggtttg aatcagatct tcctgtgaca aaatggatcg cgcgtattcg ttgatgcggc
     1201 tccgcaattc cgggattgcc tcagccggta gcgaaaatcc ggcggcggcg gcatgtcccc
     1261 catagcgatg gaacagtcca tcgtgaatat gttgcagggc atcaaccata ttaaactggg
     1321 ggatactgcg cgctgaccca tgggcgtggc tgccacgaat cgagcacacc agcgccgggc
     1381 ggtgatattg ctccaccact ttggaggcgg caatgcccac gacaccctga tgccatcccg
     1441 cctgatgctc acccgccagg acaaagacca ggtcatttgg gtcctgattc ccaatttcct
     1501 ggtgcagcat atcaaccaga ttgcgctgaa tatcctgtcg ttcgctattc caggtatcaa
     1561 tttgagccgc ccggcgtttg gcttcgtcca gcgactttga gtcaaagagg tcaacgacac
     1621 ttaacgtaga accatcaatc cggcctgcgg cattgattcg ggggccgagt cggaacccaa
     1681 ggtctgacgc cgcaatctgt tttccgctaa gacgtgccac ctccagaaga gctgccagcc
     1741 ctggattgct tgaaccactg ctcagtcctt tcaagccatt ggccacaatg gcgcgatttg
     1801 aagggctgga aagcggtacc aggtccgcca ctgttccaat ggccaccagt ttggtcagcg
     1861 actcgacaat tcggtcacgt ttcggatggt cagccagcag ggcccgtcca aattgcaggc
     1921 tcaagccaca cgccgccaga tctttttcag aatagtcgca gccagattgt ttcgggcaga
     1981 cgataatggc gtccggcgga accgagcttc ccagcacggt gtggtggtca aggcaaatcc
     2041 cggtcatgcc tcgttcacga accatcgccc attctttata ggcggtgatt ccaatatcaa
     2101 ccgaaatcag gacccggaca tcatctgttg ctgcgcgttc gacaatgccc gcgttgaggc
     2161 catagccatc acgctcacgg tccggcacgt gaaacgacaa aaattcatca gcggcgggcg
     2221 acaaaatccg gaagacattg agcaatgtga tcaaccccgt ggtgccgtca cagtcatagt
     2281 cgccgacgat acgaactttt tcccggcggg ccagcgcccc actgacaacc tgaacggctt
     2341 cctggaggtt gcgcattcca cgggggtcag gaatatctga caaacttggg ttgagaaaac
     2401 gctcggcctg ggttggggtg gaaattcccc ggtttgccag gcactgggcg agtagtggtg
     2461 ggatggagag ttcctggatg agtaaacgga tggtggcctg gtcgacctca agcagtttcc
     2521 aggacgaatg ggtgagggat aatgaagaat c
//
LOCUS       DAOQDO010000788         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35427_length_2550_cov_2.767936, whole genome shotgun sequence.
ACCESSION   DAOQDO010000788 DAOQDO010000000
VERSION     DAOQDO010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35427_length_2550_cov_2.767936"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKZ53_12785"
     CDS             complement(<1..247)
                     /locus_tag="PKZ53_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41362.1"
                     /translation="MKIPINAFRIRLICFLMTFAIGLACVEWYERLCCQSHQPKQHCV
                     NITKPLPVLPQAPVSFKSAELAQEVPVWFQNDLGASKT"
     gene            complement(406..2037)
                     /locus_tag="PKZ53_12790"
     CDS             complement(406..2037)
                     /locus_tag="PKZ53_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41363.1"
                     /translation="MTFKPTSLRLKVLCFLMTFGIGLACVEIYQTIPSQEVAEPISFE
                     SPELAHEIPDWFRYNPNDPYAVFQAWRNLTTREAIISQRDGWWVLAGILKDEAGRPVP
                     FTPIRSGCTTPIPIRGVDNGRCITDQNGYFIIYGTCDLDADMGTIERDDGLILRGGVY
                     FSVCPGYPATNQGQNFADYKKAWMGTTPQLAYRLEDRAFYVVTVAEKIPFDEQEFARF
                     LADSSEYDNKIRALTETHHQSTEVPLRQRPDFSPEKLVTYHFRLISPTGQPVPQAIVE
                     GNFLNPDSPPFDKKQTVMTDDEGKGQFEVLVPDGYQKEIFKALSRNLTIQAGKYGEGL
                     ISCQLRADRATTLKLKRPAYIYGKIIDHHGNPEKATVYLKSAKTEFEYSSGFSRQDYG
                     SLRSPSVTSKSDGTFSLGPVFSDKKVFAQTGSYYDGYVESEPFRLLPGSRKEINMTIP
                     LMASVRALVVDEASRPVNPNGNASFSIELEVAPGRTKVLDLSLGNGRLVWVDLVPKPF
                     RVTGDVPGYEPYCSDQMVLEPGEVKLLTIQLTRRK"
     gene            complement(2160..>2550)
                     /locus_tag="PKZ53_12795"
     CDS             complement(2160..>2550)
                     /locus_tag="PKZ53_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41364.1"
                     /translation="SWSRDFILALTHHPLSWLPEWDERLCRQQIETRCDFHLSGHLHE
                     THFNLRGVGGPQRCSEIAAGSAYGGSQWYNGYNLVELDFQTGKGRATLRRFEPTITAW
                     KPDIRFERLQPGQFIPFWNGVLVLQEG"
BASE COUNT          666 a          627 c          586 g          671 t
ORIGIN      
        1 tagtttttga agcccccagg tcattttgaa accagactgg cacttcctgc gccagctcgg
       61 ctgatttgaa agaaactggt gcttgcggaa gcacaggaag tggcttcgtg atattgacac
      121 agtgttgttt gggttgatga gattgacagc acaatctctc gtaccactca acacaggcta
      181 atccaatcgc aaaggtcatc aggaaacata tcaacctgat tcggaaagca ttgatcggga
      241 ttttcatcag tcaacctctt tcattgccca catatttccc aggttcaatg acactgatac
      301 cagcttgcaa tggagccctc gtcttagaaa acagtcaagt aattcaatca aatgaagtta
      361 gtgaaccact gactacgatc tactgactac aaactggtat tactttcatt ttctccgggt
      421 gagttggatt gtcagcagtt ttacttcacc gggttctaac accatctgat ctgagcaata
      481 tggctcatac ccaggaacgt ctcctgtcac tcgaaatggc ttcggaacaa ggtctaccca
      541 gaccaatcgc ccgttgccca aagataagtc taagaccttc gttcgccctg gggccacctc
      601 cagttcaatt gaaaaactgg cattcccatt tggattcaca ggccgactag cctcgtcaac
      661 gacgagggcc cggactgacg ccatcaacgg gatcgtcata ttgatttctt tccggcttcc
      721 tgggagcagt ctgaacggct ctgattcgac atacccatca taataagaac ctgtttgggc
      781 aaagaccttt ttatctgaaa acactggtcc caatgaaaaa gtaccatcag attttgaggt
      841 cactgagggt gatcgcaacg agccgtaatc ctgtcttgaa aaacctgatg aatattcaaa
      901 ctccgttttg gctgatttca ggtaaacggt agctttttcg ggatttccat ggtgatcaat
      961 tattttccca taaatgtacg ctggccgttt caatttgaga gtggttgccc ggtcagccct
     1021 gagttgacac gaaatcaacc cttctccata tttgccagcc tggatggtga gatttcttga
     1081 caatgctttg aatatttctt tttggtagcc gtcgggaaca agcacctcaa actggccttt
     1141 cccctcgtca tcagtcatta ccgtttgttt tttatcaaaa ggtggagaat ctggattcag
     1201 gaaattccct tcgacaatgg cctgcggaac tggctggcca gtcggagaga tcagacgaaa
     1261 gtgatacgtc acgagttttt ccggtgaaaa atctgggcgc tggcggagcg gaacttcagt
     1321 tgattggtga tgagtttccg tcagggcacg aattttatta tcatattcag aggaatcagc
     1381 cagaaatcga gcaaattcct gctcatcaaa gggtattttc tcggctacgg tcacgacata
     1441 aaacgcccgg tcttccagtc ggtacgcaag ttggggcgtt gttcccatcc aggctttttt
     1501 gtaatccgca aaattttggc cctgattggt ggccggatat ccagggcaaa cagagaaata
     1561 aacgccacct cgaaggatca agccatcatc tctctcgatt gtccccatat ccgcgtcaag
     1621 atcacaggtt ccataaatga taaaataccc attttgatca gtgatacacc tgccattgtc
     1681 gacacctcgg attgggattg gagttgtgca gcctgaacga atcggcgtga atggcactgg
     1741 acgtccagct tcatctttga gaattccggc cagaacccac cagccatctc tttggctgat
     1801 aatggcttca cgagtcgtta aatttcgcca cgcctgaaat acagcgtacg gatcgttggg
     1861 gttataccga aaccagtcag gaatttcatg cgccagttca ggtgattcaa aagaaatcgg
     1921 ttctgctacc tcttgggacg gaatcgtttg gtaaatctca acacaggcta atccaatccc
     1981 aaaagtcatc aggaaacata aaaccttgag ccgcaatgag gtgggtttaa acgtcatgtt
     2041 tcctccagtt caggtgattc aaaagaaatt ggttttggta cagattgaga tggaataata
     2101 ccgttttggg atcaggggct cggggtcatg ggtcagggaa atacaatctt ttcaatgatt
     2161 taaccttcct gtaatacgag gactccattc caaaatggta taaattgccc cggttgcagg
     2221 cgttcaaaac gaatgtccgg tttccaggcc gtaatcgtcg gctcaaaccg ccgcaacgtc
     2281 gcccgccctt ttccagtttg aaaatccagt tcgaccagat tgtagccgtt gtaccactgg
     2341 cttccgccgt aagctgaacc agcggcaatt tccgaacagc gttgcggtcc gccaacaccg
     2401 cgcaaattaa aatgggtttc gtgcagatgt ccgctcaaat ggaagtcgca ccgggtttca
     2461 atctgctgcc gacacagccg ttcgtcccat tccggcagcc acgagagcgg atgatgggtc
     2521 agggcaagga taaaatcacg actccaactc
//
LOCUS       DAOQDO010000789         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35472_length_2548_cov_3.539110, whole genome shotgun sequence.
ACCESSION   DAOQDO010000789 DAOQDO010000000
VERSION     DAOQDO010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35472_length_2548_cov_3.539110"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..>2548
                     /locus_tag="PKZ53_12800"
     CDS             301..>2548
                     /locus_tag="PKZ53_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ41365.1"
                     /translation="MNTIWMKPFLGRTGYWLGCVLGLVFLLTQPAWAQGTSGRLIVTA
                     KDQTGAVVAGATVTITNIGTSTAVTNTANELGVAIFPQLLVGQYTVTVEAPGFKKAAR
                     EDVKIDVGQEYSLTVGLEVGSEGEVVTVSAGEELVQTSTAEVKNTVNEKQVQDLPING
                     RDVLQLIQLQAGVASGSNSAQDNAYTVINGQRAGTASVTQDGINIQDNYFRENSLDFT
                     PNRPTVAQVAEFTVTTQNGDVDVSGGASAVRTISPSGTNEFHGQVFEYHRNSALGAND
                     FFNNLQELEKPQLIRNQFGFTLAGPAIKDKLFFFMFYEGVKERRSVPQTNTILLPNAR
                     QGVFTYRDNSGQTRTINLLGLRDFDADPAMAAIINQLPTSGNSTAFGDGLNTTGFSLN
                     RSFNRDRNSGGFRVDYVINPSHRIEGIYNRAYEMVDRPDADLLFTQKPLVNNVTDTNF
                     AVGAWTWVVNPRLTNELRIGGNRPYGVFNSKVDLNSVYYDLPLISNPVNTFPAQGRTT
                     TPISFIDSASYTIGAHFMRFGGQINRVRTDPFIFNNGTPPLFTIGFGAGAPDDAPLTA
                     QDFPGGIDASQLSTANGLLALLAGVVSEGVVGYEVTSQTSGYQTGIPNRRNLEYKEYS
                     GYFTDSWRIHPRVTLNLGLRWDYRSPLKERDNLLLAPVLNGRNVLDAVLDPNGTLDFV
                     KGGVNNPDRDNFAPNISFAWDIPGLGRQTVLRGGYSISYVNDQSFIGPYNALRGNDGL
                     QAAVNINDL"
BASE COUNT          595 a          627 c          693 g          633 t
ORIGIN      
        1 tggcgtttgc caatgtggcg agcccctcgt ttaacaaccc gcgtgtgttt gcgggacgtc
       61 cgcggacgat tcagtttggg ctcaaactga tcttttagga tgtgaggcag tgttggggac
      121 gtgaacttca agctaaagcc aggtccatcc gggctggagt caaggtgttg attgggtttc
      181 tgagttgatt gtgggatgtc agaaaagaaa tccacaccct tcctctggtc tggtgatctg
      241 gtcactggtt gcaaggaatc gtgtttgacc tgcctcctcc tttttttcaa tggagggaat
      301 atgaatacaa tttggatgaa acccttcctg ggacgcaccg gatactggtt gggatgtgtt
      361 ctgggactgg tgtttttgct gacacagcct gcctgggcgc agggtacaag cggacgtctc
      421 attgtgacgg ccaaagacca gaccggcgct gtcgtggctg gtgccaccgt aactattaca
      481 aatattggga cgtcaactgc ggttacaaac accgcaaatg agcttggcgt cgccattttc
      541 ccgcaattgc tggtggggca atataccgtg accgttgaag ctcccggctt taagaaagcg
      601 gcccgcgaag acgtcaaaat tgacgtgggt caggaatata gcctgacggt tgggcttgaa
      661 gtcggctctg aaggcgaagt ggtaacggtt tcagccgggg aagaactggt gcaaacctca
      721 accgccgaag tcaaaaatac agtgaatgaa aagcaggtcc aggatttgcc aatcaacggt
      781 cgggacgtgc tgcaactcat tcaattgcag gcgggtgtgg ccagtggcag caatagcgcc
      841 caggataatg cctataccgt catcaatggc cagcgggcag gtacggcgtc agtgacccag
      901 gatggaatca acattcagga taactatttc cgtgaaaatt cactggattt caccccgaat
      961 cggccaacgg tggctcaagt agctgaattt accgtgacaa cccaaaacgg tgacgtggat
     1021 gtctcgggag gtgcgtcagc ggttcgaacg atttcaccat ctggaacgaa cgagtttcat
     1081 gggcaggtct ttgaatatca tcgcaacagc gcgcttggtg ccaacgactt tttcaataac
     1141 ctgcaggaat tggaaaagcc acaactcatc cgcaaccagt ttggctttac gctggcaggc
     1201 ccggccatta aagacaaact gttcttcttt atgttttatg aaggtgtaaa ggagcgtcgg
     1261 agcgttcccc aaaccaacac catcctgttg ccaaatgccc gccagggagt ctttacctat
     1321 cgggacaata gtggccagac tcgaacgatt aacttattgg ggttacgtga ttttgatgct
     1381 gatccggcga tggcggcgat catcaatcag ctcccaacaa gcgggaattc aaccgccttt
     1441 ggggatggac tcaacaccac cgggttttct ctgaaccggt cctttaaccg ggatcgaaat
     1501 tcaggtgggt tccgagttga ttatgtgatt aatcccagcc atcgaattga agggatctat
     1561 aaccgggcat atgaaatggt ggatcggcct gatgctgatt tgctgtttac ccaaaaaccg
     1621 ctggtcaata atgtcactga caccaatttt gccgttggcg cctggacctg ggtagtcaat
     1681 ccgcgattga ccaatgaact gcggatcggc ggcaaccggc catatggagt gtttaattcc
     1741 aaggttgatt tgaattcggt ctattatgac ctgccgctca ttagcaaccc ggtcaataca
     1801 tttccggctc aggggagaac caccacccca atttcattta ttgacagtgc ctcctacaca
     1861 atcggggcgc actttatgcg gtttggcggc caaatcaacc gtgtccgaac cgacccgttc
     1921 atcttcaaca acggcacccc tccgttgttt accattggat ttggggcggg ggcgccggat
     1981 gatgcgcctc tcacggccca ggatttcccc ggcggaattg atgccagtca gctttccacc
     2041 gcaaacggtt tgctggcgtt gctggctggg gtcgtttcgg aaggtgtggt tgggtatgaa
     2101 gttacatccc agacctcggg ataccagact ggaattccca atcgccgtaa ccttgaatat
     2161 aaggagtatt ccggctattt caccgattcg tggcgcattc acccgcgtgt gacactcaat
     2221 ttggggttgc ggtgggatta tcgttcccct ctgaaagagc gtgataacct gttgctggca
     2281 ccagttctca atggccgcaa tgtacttgac gccgtgcttg atccaaacgg aacccttgat
     2341 tttgtgaaag gaggcgtcaa taacccggat cgggacaact ttgcgccaaa tatcagcttt
     2401 gcctgggata tcccaggtct tggccgccaa accgtattgc gcggtgggta ttcgatttcc
     2461 tatgtcaatg accagtcatt tattggaccg tacaatgccc tgcgaggaaa cgatgggttg
     2521 caggcagccg tcaatattaa tgacttgt
//
LOCUS       DAOQDO010000790         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35538_length_2546_cov_3.485347, whole genome shotgun sequence.
ACCESSION   DAOQDO010000790 DAOQDO010000000
VERSION     DAOQDO010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35538_length_2546_cov_3.485347"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="PKZ53_12805"
     CDS             <1..1973
                     /locus_tag="PKZ53_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ41366.1"
                     /translation="PTLEPGTRNPEPGTLAFAVFLGDQLPEYMIPVAFVVLEQFPMLP
                     NGKVDRKALLRVPVAIEAPAKEVTLPQTQIEETLARIWAQVLRVPQVGVHDNFFELGG
                     DSILTIQIVARAGQAGLKLSPKDIFQRQTIAELATIVEAGALISNEQGLISGTVPLTP
                     IQHWFFEQPLSNPHHWNQAFLFEVPLALETEKLKLAVKHVLDHHDAVRMRFTKTDTGY
                     VQTNLSAEPGEVVRFHDLSDVPDEGLASEIEQIAQAEQASLHTSDGPLVRVTYLATGL
                     GRTNRLLVVIHHLVIDAVSWRILLEDLISAYRQLSQNQSVQLPAKTTSFKDWAERLHI
                     YAQSPALTQEAPFWFNTLKPPVVRIPVDFIKSENTVASAAEVVGALDEAQTTALLRQV
                     PKAFGTQINEVLLTAVTRALSDWILETLPTSTTFNNVTDFPPATRNPEPGTRNPALLI
                     DFEGHGREELFPDVNLSRTIGWFTTLFPVRLELSTTQPVPEHLKTIQQRLQQIPNRGI
                     GYGILRYLNQDHSLQAQFSAAAHAEIAFNYFGQLDAHPGTATGVPGFRVAPESPGSPA
                     DAQGIRSHLIEVQASITGGKLKMVWTYSQNLHSPVIISRLSHLCLDTLTTLISQTQLA
                     AQSRMVQPEDFPLANLDKNKLGKVLSQLGKKK"
     gene            2058..>2546
                     /locus_tag="PKZ53_12810"
     CDS             2058..>2546
                     /locus_tag="PKZ53_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ41367.1"
                     /translation="MHTLKITHRPMMFLAAFFLLMSPLFVQAAPVQEEAKELQEHYSQ
                     LDKAIKDKKIDAMASVMTEDFKFQSKDGKSFTNKQTLQLTEQMFSALQTVHESVSKVE
                     KVEEGDTATTLVATVKQTLKGTITGPDGKPHELVSITVSKDQWVDTEDGWKMKASTTI
                     EET"
BASE COUNT          695 a          699 c          583 g          569 t
ORIGIN      
        1 ctccgactct ggaacctgga acccggaacc cggaacccgg aaccctggct ttcgcagttt
       61 tccttggcga ccagttacca gagtacatga ttccggtggc atttgtggta cttgagcaat
      121 ttccaatgct cccgaatgga aaagttgatc gaaaagcatt gctccgggta ccggttgcca
      181 ttgaagcacc agccaaagaa gttacgcttc cgcaaaccca gattgaagaa accctggccc
      241 ggatttgggc acaggtgctg cgggtaccgc aggttggggt tcacgataat ttctttgaac
      301 tcggcggaga ctcgatcctg accattcaaa tcgtggcgcg ggcaggtcag gccggcctga
      361 aactgagtcc aaaggatatt ttccaacgcc agacgattgc cgaactcgcc acaattgtcg
      421 aagctggcgc cttgatttcc aacgaacagg gactgatttc gggaacggtg cccctgacac
      481 caatccagca ctggttcttt gaacaaccct tgtcgaaccc ccatcattgg aatcaggcat
      541 ttctctttga agtgccgctc gccctcgaaa ctgaaaagct caagcttgcg gtgaagcacg
      601 tgctggatca tcacgatgcc gtgcgaatgc gctttaccaa aactgacacg ggctatgtcc
      661 aaaccaacct gtcggcagaa cctggagaag tcgtccgatt ccacgatctt tcagatgtac
      721 cagacgaggg gctggcttct gaaattgaac agattgccca ggcggagcag gcatcgcttc
      781 atacctcaga cggtccgctg gtacgggtca cctacctggc aaccggcctt ggacgaacca
      841 atcggctgct ggtggtcatc catcatcttg tgatagatgc tgtctcgtgg cgaatcctgc
      901 tcgaagacct gatttcggcc tatcgccaac tcagtcaaaa ccagtcagtt caacttccag
      961 ccaaaaccac atcgtttaag gactgggccg aacggttgca catatatgct caatcaccag
     1021 ccttgaccca ggaagccccc ttctggttca acacgctcaa accacctgtg gttcgaattc
     1081 cggttgattt tattaaaagt gaaaacacgg tcgcatcggc ggctgaggta gtgggggcac
     1141 ttgatgaagc gcaaaccacc gccctgttgc ggcaggtgcc caaagccttt ggaactcaga
     1201 tcaacgaagt gttactgaca gcagtcacca gagccctttc ggattggata cttgaaacgc
     1261 ttcccacatc aacaactttc aataatgtga ctgacttccc cccagcaacc cggaacccgg
     1321 aacccggaac ccggaacccg gctctcctga ttgatttcga aggccacggt cgtgaggaac
     1381 tcttccccga cgtcaatctt tcgcgaacca tcggctggtt tacgacgttg ttcccggttc
     1441 gtctggaatt gagcaccacc caacccgttc ctgagcacct caaaacgatt cagcaaaggc
     1501 ttcagcaaat tccaaaccgt gggattggtt atggaatcct ccgatacctg aatcaagatc
     1561 actcactgca ggcacaattc tcggcagccg cccacgctga gattgccttt aactattttg
     1621 gtcaactgga tgcacaccct ggcacggcaa caggtgtacc tggattcaga gtagcaccag
     1681 agtcaccagg atcacctgcc gacgcacagg gtattcgaag tcatttgatt gaagttcagg
     1741 ccagcatcac aggtggaaag ttgaaaatgg tgtggaccta tagccagaat ttacatagtc
     1801 ctgtaattat ttcgcgactt tctcatctgt gtctggatac gctgacaaca ctcatttccc
     1861 aaacccaact tgctgcccaa tcccgaatgg ttcaaccaga agacttcccg ctggccaatc
     1921 tggataaaaa caaactggga aaggtgttgt cccagttagg taagaaaaaa tgaactcaat
     1981 aaaatgaatt tctcattccc cctgtttgtt taaaaggaac caaacgtcac aactcacaca
     2041 aggagtcatc cgcacctatg cacacactga aaattacaca ccggccaatg atgtttctcg
     2101 ctgcgttctt tctgcttatg tcacctcttt ttgtccaggc agcacccgtt caagaagaag
     2161 cgaaagaact acaagaacat tattctcaac tcgataaggc cattaaagat aaaaaaattg
     2221 atgccatggc ctcggtcatg accgaagatt tcaagttcca atccaaagat ggcaaatcct
     2281 ttaccaacaa gcaaaccctg caattgacag aacaaatgtt ttccgctctg caaaccgtcc
     2341 acgagtcagt gagcaaggtt gaaaaagttg aggaaggtga cactgcaaca acactcgttg
     2401 caaccgtgaa acaaacgctc aaaggaacca tcactggccc cgatggaaaa ccacacgaac
     2461 tggtttcgat cacagtttcg aaagatcagt gggttgatac cgaagatgga tggaaaatga
     2521 aggccagcac caccattgaa gaaacc
//
LOCUS       DAOQDO010000791         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35573_length_2545_cov_2.568273, whole genome shotgun sequence.
ACCESSION   DAOQDO010000791 DAOQDO010000000
VERSION     DAOQDO010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35573_length_2545_cov_2.568273"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKZ53_12815"
     CDS             <1..1020
                     /locus_tag="PKZ53_12815"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HNJ41368.1"
                     /translation="GLLKQSLKLLVENLKPGDTVAITTYAGGTSTVLTPTGIDRRNEI
                     LAALDRLQAGGSTAMESGIDLAYRQAASVNRPGMINRIVICSDGDANVGRTSPDEILK
                     TIEGYVKEGITVSTIGFGMGNYKDTMMERFADKGNGNYFYIDTFDQARRVFVDELTGT
                     LQVIAKDVKVQVEFNPAAVSQYRLIGYENRDVADEDFRNDRVDGGEIGAGHRVTALYE
                     LELAPNPAPKLATVRLRYKQPEGQSAQEVLAEFATSRIPAQFAQTSEDFRFAVAVAAF
                     AEVLRGSEQARNWSLQTVAQLARDSSASDNAERQELIGLIAKAQTLQDRSKTAPASPS
                     GYPLQ"
     gene            1227..2045
                     /locus_tag="PKZ53_12820"
     CDS             1227..2045
                     /locus_tag="PKZ53_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ41369.1"
                     /translation="MAKELILLEIENKVAVLKLNRPTVLNALNLEMWKILDSSLSELE
                     QLGDEVRAVIITGVGERAFCAGLDLSPDNPIVWDLLSQANHDRRAQIADELNWLRDTF
                     NRLADLPMPTIAALNGLAYGSGLELAMCCDVRIAAENIQLCLPEVSVGVIPDKGGTQR
                     LPRLIGLARAKEMILTGLPVTSDEGARIGLVNRVVPADQLMNAAVEYAHRIAQMSPLA
                     IRAAKQALNQTQSLPLADGFRFENRMALEAILSEDLAEGIRAFQEKRPPVFRGV"
     gene            2302..>2545
                     /locus_tag="PKZ53_12825"
     CDS             2302..>2545
                     /locus_tag="PKZ53_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41370.1"
                     /translation="MSDKACFSCGRMNPESADVCVFCRASFNTIPFEPVPVPEPDEPA
                     ASHRQPFFLPVSPLSEQYYHSSGKAPVSGVVLSLMGG"
BASE COUNT          583 a          611 c          705 g          646 t
ORIGIN      
        1 gggttgctca aacaaagcct gaaattgctg gtcgaaaact tgaagccggg cgatacggtg
       61 gccattacga cgtatgccgg aggcacgagc accgttttga ctccgactgg gattgatcgc
      121 cgcaatgaaa ttctggcggc gctggaccgt ctccaggctg gtggctccac cgcgatggaa
      181 tccgggattg accttgccta ccgtcaggca gcctcggtca atcgtccagg tatgatcaac
      241 cggattgtga tttgctcaga cggagacgct aacgttggcc gaaccagccc tgacgaaatt
      301 ctcaaaacca ttgaaggcta tgtcaaagaa ggcattaccg tcagcaccat cggttttgga
      361 atgggcaatt ataaagacac aatgatggag cggtttgccg ataaaggaaa cgggaattac
      421 ttctacattg atacttttga tcaggcccga cgggtatttg tggatgaatt gactggcacg
      481 ctccaggtga ttgcgaaaga tgtcaaagtt caggttgagt ttaatccagc cgctgtttca
      541 cagtatcgcc tgattggtta tgaaaaccgt gatgtggctg acgaagattt tcgcaatgat
      601 cgcgttgatg gtggcgaaat tggtgctggt caccgggtaa cagcgctcta tgaactggaa
      661 ttggcaccta acccggcgcc aaaactggcg acggttcggc tgcgatataa acaaccagaa
      721 ggtcagagtg cgcaagaggt tttggccgaa tttgcgacct cgcgcattcc agcccagttt
      781 gctcagacgt cagaggattt tcggtttgct gttgccgtcg cagcttttgc cgaagtgttg
      841 cgtggtagcg aacaggctcg gaactggtcg ctccaaactg ttgcccaact ggctcgtgac
      901 tcgtcagcgt ctgacaatgc agaacgccag gaactgattg ggttgattgc caaagcccaa
      961 actttgcagg atcgcagcaa aactgctccg gcgtctccgt caggttatcc acttcagtaa
     1021 gggaggccgg gttttcaaaa gagcatcctg cactgcacct gcgtcattac ctcatccagt
     1081 cgctttgtca ttgagtcatc ggttcagggg gttgcaagtt gggcaccggc ccatataact
     1141 tgcagccctc ttcttttggt actgtttcca cacgtgatgc tttggttcct gtttcccaat
     1201 ctgatgccgt tgtttggggt gtttttatgg cgaaagaatt gattttgctt gagattgaaa
     1261 acaaggttgc ggttctcaaa ctgaatcggc caactgtcct caatgccctc aatcttgaaa
     1321 tgtggaaaat tctggactca agtctgagtg agttggaaca actcggagac gaagtccggg
     1381 cggttatcat cactggtgtt ggtgagcgtg cgttttgcgc tggacttgac ctgtcgccag
     1441 acaatccgat tgtgtgggat ctgctgagtc aagccaatca tgatcggcgt gcccagattg
     1501 ccgatgaact caactggttg cgtgatacct tcaatcggct ggctgacctg cctatgccga
     1561 cgattgcagc gctcaatggg ctggcctacg gctctggatt ggaactggcg atgtgctgtg
     1621 atgtgcggat tgcggctgaa aacattcagt tgtgcctgcc tgaagtttca gtcggcgtga
     1681 tcccagataa gggtggaacc cagcggttgc cacgcctgat tggtctggcc agagccaaag
     1741 aaatgattct cactggattg ccggtgacca gtgatgaagg agcccgcatc ggactggtca
     1801 atcgggtggt gccagccgat caattgatga atgccgctgt cgagtacgcc catcggattg
     1861 cccagatgtc acccctggcg attcgggctg ccaagcaggc cctcaaccag acacaatctt
     1921 tgcccctggc tgatgggttc cggtttgaaa atcgaatggc actcgaagcc attttgtctg
     1981 aagacctggc tgaaggcatc cgggcatttc aggaaaagcg accacccgta tttcgagggg
     2041 tgtagtctca gagttggatg aaactttttt cttccaaacc agaataaaag atagacaaac
     2101 ccggcgttgt gcagtaaaga aacactattc ctcacttgtg tttcaattct gaagaaaggg
     2161 ttagccgtac agcgcgccgt ctatgacgat caacccttaa aaaagcgcgt ttagtgagca
     2221 aaatcgttca ttttggtact gatagccagg ttttacagcg agttgggagc caggaatgcg
     2281 cctgctgtac gctcgagtgc tatgtctgac aaagcctgtt tttcctgtgg taggatgaat
     2341 cctgaaagcg ctgatgtgtg tgtattctgt cgggcttcat tcaatacaat tccatttgaa
     2401 ccagtgccgg tacctgagcc tgatgagcca gccgccagtc accggcaacc attctttctg
     2461 ccagtttcgc ctctgagcga gcagtattac cacagcagtg ggaaggctcc agtttctggg
     2521 gttgtactca gcttgatggg tggtg
//
LOCUS       DAOQDO010000792         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35580_length_2545_cov_2.306024, whole genome shotgun sequence.
ACCESSION   DAOQDO010000792 DAOQDO010000000
VERSION     DAOQDO010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35580_length_2545_cov_2.306024"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..664
                     /gene="purN"
                     /locus_tag="PKZ53_12830"
     CDS             59..664
                     /gene="purN"
                     /locus_tag="PKZ53_12830"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNJ41371.1"
                     /translation="MSLPNIAVLISGRGSNMMALASAIDRGELHARLACVVSNRADAP
                     GLKLAADRQIPTCVLPHQGLSRQAHGRQIREALAPHQISLICLAGFMRLLDGEFIQAY
                     KNQIVNIHPSLLPAFPGLHAQAQAFEYGVKVTGCTVHFVDEELDHGPIIAQTAVLSQD
                     TDTQETLAQRILDAEHRLYPAAVEKILHSRWTIQERRVIFG"
     gene            1048..2262
                     /locus_tag="PKZ53_12835"
     CDS             1048..2262
                     /locus_tag="PKZ53_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41372.1"
                     /translation="MKLCPKCNTQWDDLKRFCPYDGTFLPSLAHLPVESTSEPTNSTS
                     DFRASQIGYAVHQPQPTEALSEGTPVLAMTGTPISVSATPSAQPPASPPSPALWTTDD
                     TPTVKASHPHAVWPTSYQPGQTPREGIPAIPANDDQLFPLPEASVSNSGPVTRETPAA
                     PVTAPEMPFTPGAWGGDSPEDVGFLFDAQHQAPAKNPDAVPTVPAAAPGELPGGIIHR
                     DLEATAKASPAEIPKTVSVFEVAREPRDRMRRSQGMPALVEPSRPKRTHAEYFQLLNE
                     RNRIIQQFVEVLVKQGFSYKSSYSNKDDHLIYRFDLSFGDADDHRTFPVSVALHRKPS
                     YSVLVSIDLFEIGRTLPERTKRTELIGGRVERTDHGMYFHIDATEDLPNEYLMQWLDE
                     NLKSIFRITYET"
     gene            complement(2365..>2545)
                     /locus_tag="PKZ53_12840"
     CDS             complement(2365..>2545)
                     /locus_tag="PKZ53_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41373.1"
                     /translation="CGGLTLHKLVQKKGIGVTLLKIESGVKPPHCKSIPLILVVTNSF
                     LGTFFIQRQDERLTG"
BASE COUNT          614 a          767 c          567 g          597 t
ORIGIN      
        1 ttcttcattc ttcattctca attcttcatt cttcattctc aattcttcat tctcaggtat
       61 gagtctcccc aacattgcag ttctgatttc aggtcgcgga tcaaatatga tggcgttagc
      121 cagcgccatt gatcgcggtg aactgcatgc ccggctcgcc tgtgtcgtga gcaaccgggc
      181 agatgccccc ggcctgaaac tggcagccga ccgacagatt cccacctgtg tccttccgca
      241 tcaaggactt tcccgtcagg cccatggccg ccaaattcgc gaagccctgg ccccccatca
      301 gatttcactg atttgcctgg caggttttat gcgacttctt gatggagagt ttatccaggc
      361 gtacaaaaac cagattgtga atatccaccc atccctctta ccggcctttc caggcttaca
      421 cgcccaggcc caggcatttg aatatggtgt caaagtgacg ggctgtaccg ttcattttgt
      481 ggatgaagaa ctcgatcacg gccccatcat tgcccaaacc gccgttctgt cccaggatac
      541 ggatactcaa gaaactctcg ctcaacgcat tcttgacgct gaacaccgcc tctaccctgc
      601 cgccgttgaa aaaatcctcc attccagatg gaccatccag gaacggcgtg tcatttttgg
      661 ataaccgttt ctgactgagg aaagtccaac tcacactggt cagaccgatc accagccaca
      721 cccaaccgcc aatttcgtct ggcagcacac aatcttcttg aaaaaaagtg aaaatcaatg
      781 aaaatacctg cacccaggta ttttcaatcg ttcgcactca cattcaactc caactcaatt
      841 tcccgttccg ccgctttgtt tttcaagtca ggtaccgtgc gtatcgtttt gaccagcctt
      901 gagcagcaag tgatggaatt actttgggtt tgatggactc tggtccagag cacacaaggt
      961 gggtgttatg tcgtctcacg ttttctcaac taatgaagga cagctatcca ctcacccctt
     1021 ttcgattggt ggatggctga aagcgcgatg aagctgtgtc caaagtgcaa cacgcaatgg
     1081 gatgatttaa aacgattttg tccatacgac ggaacatttc tcccgtcact ggctcacctg
     1141 cctgtggaat ccacgtccga gccgaccaac agcaccagcg atttccgcgc ctctcagatt
     1201 gggtatgccg ttcatcagcc ccagccaaca gaagcgctct ctgaaggcac tccggttttg
     1261 gcaatgacag gtaccccgat ttcagtttca gccactccct ctgcccagcc accagccagc
     1321 ccaccatcac cagccttgtg gaccaccgac gataccccaa cggtgaaggc cagccatccc
     1381 catgccgtct ggcccacgtc ctatcaaccg ggtcagactc cgagagaagg gatccccgcg
     1441 atcccggcta acgatgatca gctctttcca cttccagaag catccgtttc caattcgggg
     1501 ccagtcacca gagaaactcc agccgccccg gtgaccgcgc ctgaaatgcc attcacacct
     1561 ggggcctggg gcggggactc acccgaagat gtcggatttc tctttgacgc acagcatcag
     1621 gcaccagcca aaaatccaga tgcagttcca acggtaccag ccgcagcccc cggagaatta
     1681 cctggaggaa tcatccaccg tgacctggaa gcaaccgcca aagcttcccc ggctgaaatc
     1741 ccgaaaaccg tgagcgtttt tgaagtcgcc agagaacccc gagaccggat gcgccgctcg
     1801 caaggtatgc cagccctggt ggaaccgagt cgtccgaaac ggacgcatgc cgagtatttc
     1861 cagctcctga acgaacgaaa ccggattatc cagcagtttg ttgaagttct ggtcaaacaa
     1921 ggcttttcat acaaaagcag ttatagcaac aaagatgatc acctgattta tcgctttgat
     1981 ctttcattcg gagatgccga cgatcatcgg acgtttcccg tctcagttgc cctgcaccga
     2041 aaaccgtcgt attccgtcct ggtttcaatt gatctgtttg aaattggccg aacacttcca
     2101 gaacgcacca agcgcacaga attgatcggt ggacgggtgg aacgaaccga ccatggcatg
     2161 tactttcata ttgatgccac tgaagatctt cccaacgaat acctgatgca gtggttggat
     2221 gaaaatctca agagtatctt tcgcatcacc tatgagactt gaaaaagagc gaaagagcga
     2281 aaggacccgg gtagccagga atcaatctcc agccccttgt tatcttgtta ttctgccacc
     2341 tgtcacctgc caccctgtca cctgtcagcc tgtcagcctc tcatcctgcc tctgaatgaa
     2401 gaatgtcccg agaaatgagt tggtgacgac caaaatcagc ggtatacttt tgcagtgcgg
     2461 cggcttgacg ccgctttcga tcttcagcaa cgtcacacca atcccctttt tctgtaccag
     2521 cttatgcagc gtcaagccgc cgcac
//
LOCUS       DAOQDO010000793         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35590_length_2544_cov_3.304942, whole genome shotgun sequence.
ACCESSION   DAOQDO010000793 DAOQDO010000000
VERSION     DAOQDO010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35590_length_2544_cov_3.304942"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..463)
                     /gene="ndhC"
                     /locus_tag="PKZ53_12845"
     CDS             complement(59..463)
                     /gene="ndhC"
                     /locus_tag="PKZ53_12845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNJ41374.1"
                     /translation="MLAFWFALSDELVQSTASINYLPILLLLGIAVLIPVGALTVGRF
                     LRQTNETPEKMMAYECGSEPVEDARGRFSVRYYMVAMLFLVFDVEILFLFPWAIIYDQ
                     LAWFGLIEMVVFLGILVVGYYYAWRKGALQWG"
     gene            complement(796..1182)
                     /gene="rplS"
                     /locus_tag="PKZ53_12850"
     CDS             complement(796..1182)
                     /gene="rplS"
                     /locus_tag="PKZ53_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNJ41375.1"
                     /translation="MNQLVDSIEKAQMRQHPEFIAGDTIRVHVRIKEGDKERIQLYEG
                     VVIARKHSGVRETVTVRKISFGIGVERIFPLHATVIEKIEVTKRGRVRRAKLYYLRAL
                     RGKAARIRERDTRVTAQAKAAAQAKR"
     gene            complement(1249..2040)
                     /gene="trmD"
                     /locus_tag="PKZ53_12855"
     CDS             complement(1249..2040)
                     /gene="trmD"
                     /locus_tag="PKZ53_12855"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNJ41376.1"
                     /translation="MRFDILTIFPKFFEGFLTHGIVRRAHEADRIQVGIHDIRDYTFD
                     RHHVVDDRPFGGSDGMVLKPEPIFRAVEAITHPEWSLPSDTNPNRDQSGVSIILLTPQ
                     GRLFTQAEATRLSQKSQIVLICGRYEGVDERVARHVATEEISIGDYVLSGGEVAAMVV
                     VDAVTRLLPETLGSSTSAVNESFSAGRLDYPVYTRPASYRGYDVPKELLTGHHGEIDK
                     WRRRKALEKTLAYRPDLLSQRDCLDEVDQKILQDLLARKSNHTTE"
     gene            complement(2054..>2544)
                     /gene="rimM"
                     /locus_tag="PKZ53_12860"
     CDS             complement(2054..>2544)
                     /gene="rimM"
                     /locus_tag="PKZ53_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNJ41377.1"
                     /translation="LRGEVITHLLTDFPEHFDELEDVWLQYPSGDVKPVKLEESWFQN
                     GTLILKFEGIDTRTQAEALAKCKVQIPSDERVELEDDEFFVDSLIGCQVELADGQRVG
                     QVVDVLAVGGGDTLVVHSPGTDASDEEREHLIPFVESICSQVDVEARRIVVTPPEGLL
                     EL"
BASE COUNT          672 a          689 c          604 g          579 t
ORIGIN      
        1 aaccctgaac cctgaaccct gaaccctgaa ccctgaaccc tgaaccctga acccaaggtt
       61 acccccactg caaggcacct tttcgccagg catagtagta cccaaccacc aaaattccaa
      121 gaaagacaac catctcaatc agcccaaacc acgcaagctg gtcatagatg attgcccagg
      181 gaaacagaaa caagatttca acgtcaaaca ccaaaaacaa catcgcgacc atgtagtatc
      241 gcaccgaaaa tcggcctcgg gcgtcttcca ctggctctga accacactcg taagccatca
      301 ttttttcagg cgtttcgttg gtctgtcgca aaaaacgccc aacagtcaag gcaccaactg
      361 gaatcaatac agcaattccc agcaaaagaa gaatgggcaa ataattgatt gaagccgttg
      421 actgaaccag ttcatcagag agtgcaaacc aaaaagccag catagcgaca aaatctcctg
      481 caatggggaa taaaagcgat aatggacacg atctggagtt ggggcacttg tttgcggatt
      541 ggacttgaat tgtaaatgga attctatcaa cgaattgcag aaagaaacaa gaaaactggc
      601 tgattttttt gcgggatgaa ggacggaaag cggccagggt accgaaattc aggttaaccg
      661 ggcaagccgt ttgcgaccag caacgaaaaa agactccaaa aaccaaatca gtttttggag
      721 tctttttggg ttcatcctgg tcgtgatttg cccagaattc cagcgtggtc gccacgccgg
      781 gatttctgat ctgacctagc gcttggcctg ggcagcagcc ttggcctgag cggtaacgcg
      841 ggtatcgcgt tcccggatac gcgctgcttt tccgcgcaag gcacgcaggt aatagagttt
      901 ggcgcgacgc acgcgaccac gcttggtgac ttcaatcttt tcaatcacgg tggcatggag
      961 cgggaaaatg cgttcgactc cgattccgaa gctgatttta cggaccgtga ctgtttcacg
     1021 aactccgcta tgcttacggg caatgacaac tccttcgtaa agctgaattc gttctttgtc
     1081 accttcctta atgcgaacgt gaacgcgaat tgtgtcaccg gcaataaact ccggatgctg
     1141 acgcatctgc gctttctcaa tgctatcaac caactggttc atcatgatgg cggtacctcc
     1201 ctagtagtgc tgtctcaaat ccggcgtcat ctcatccgga aaaattattt attcagtagt
     1261 gtggttactt ttgcgggcta acaaatcctg cagtattttt tggtctactt cgtccaggca
     1321 atcacgctga ctgagcaaat caggtcggta ggccagcgtt ttttcaagag ctttgcggcg
     1381 acgccattta tcaatttcac catggtgccc ggtcaggagt tctttgggga catcatagcc
     1441 acgataactg gcaggccggg tgtaaaccgg ataatcgagt cgtccggcgc taaatgactc
     1501 gttgacggct gaggtcgaac tccccagcgt ttcaggtaga agccgggtaa cggcatctac
     1561 tactaccatt gcagccactt ccccgccgga aagcacatag tcgccaatgg atatttcttc
     1621 agtggcaacg tggcgtgcaa cccgttcgtc aacgccttca tagcgaccgc aaatcaaaac
     1681 aatctgtgat ttctgactga gccgggtggc ttctgcctga gtaaacaggc ggccttgcgg
     1741 ggtgagcaga atgattgaca ccccagattg atcacggttg gggttggtat cagacggcag
     1801 agaccactcg gggtgggtaa tcgcctcaac ggcccgaaaa atcggctctg gtttgagcac
     1861 catgccatca cttcccccaa aggggcgatc atcaaccacg tgatgacgat caaacgtgta
     1921 gtcacggatg tcgtggattc ccacctggat ccgatctgct tcgtgcgccc gccgcacaat
     1981 gccatgcgtc agaaaccctt caaagaactt tggaaaaatt gtcaggatat caaaccgcat
     2041 gccgcgcaca tctttaaagt tcaagcaacc cttcgggtgg cgtgaccaca attcgtcgcg
     2101 cctcgacatc aacctgcgag caaatgcttt cgacaaaagg aatcagatgc tcgcgttctt
     2161 catccgaagc gtcagttcct ggggaatgaa ccaccagtgt atccccgccg ccgaccgcca
     2221 gaacatccac cacctggcca acccgctgac catcggccag ttcaacctgg cagccaatca
     2281 gtgaatcaac aaaaaactcg tcgtcttcaa gctcaactcg ttcatccgaa gggatttgca
     2341 ctttgcactt ggcaagggct tcagcctggg ttcgggtgtc aattccttca aatttgagaa
     2401 tcaacgttcc gttttgaaac cacgattctt cgagtttgac aggtttgaca tcaccagatg
     2461 gatactgcaa ccagacgtct tcaagctcgt caaaatgctc gggaaaatcg gtcagcagat
     2521 gagttatcac ttccccgcgt aacc
//
LOCUS       DAOQDO010000794         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35601_length_2544_cov_2.698674, whole genome shotgun sequence.
ACCESSION   DAOQDO010000794 DAOQDO010000000
VERSION     DAOQDO010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35601_length_2544_cov_2.698674"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKZ53_12865"
     CDS             <1..212
                     /locus_tag="PKZ53_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41378.1"
                     /translation="AATLVQLGRWEGKQYKFGMREDILRFAPFRGAVRPAEEAVFHKV
                     QTDILTGSLNISTEIGALSSEVQGN"
     gene            212..2209
                     /locus_tag="PKZ53_12870"
     CDS             212..2209
                     /locus_tag="PKZ53_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008319192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ41379.1"
                     /translation="MPQSVQPSPLAVSINPWSSIANQLRFGLVVIVALALLVTGSLLI
                     YSSFQTLIQETNSLQRERSRVAAETIDAYLDDLLRKLNYLARVRGLTDLPTETKQTML
                     EGLARHNQAYELIAILDREGNVQSSVSPYGGAKPEPMRNSSLFIRTFLKHEDFVADVT
                     IDPDLNQPVTTFAVPIRNAQDSVDGVLLARINLKFLNFVTSQTEVGRTGYTYVVDARN
                     RLISRKGTTGQKIHLEDVSRLFFLQDLTITKARSLAVYHGLYDAEVLGAVAPVRNVNW
                     LVVVELPTAEAYAPVRRQILTMGLSLLVAVIGATGVGFLFAQRVVVPFQRLTHAAQRI
                     RDGNLEVQVHIPDRNELGVLGQAFNTMAAQLQASFQALEQMNEDLEARVVLRTAELQA
                     AFQEIETLNQQLKAENLRMGAELEVTRQLQQMLLPREAEIQTIGDLDIAGFMEPADEV
                     GGDYYDVLQHNGRIKIGIGDVTGHGLESGVVMMMAQTAVRTLLVNNETDPVKFMNTIN
                     RVIYENTHRMRSARNMTFALLEYQAGVFRLSGQHEDVLVVRHSGQVEKIDTFELGFPL
                     GIEPDISPFVAQLDIELAGGDVLVMYTDGITEAVNSDQEQYGIDRLCAVLIAHREASA
                     VELQQQVIADVKAFIGSQKIFDDLTLLIMKKKSADLNTSHL"
BASE COUNT          611 a          665 c          673 g          595 t
ORIGIN      
        1 aagccgcaac gctggttcaa ttgggacgct gggaggggaa acagtacaag tttggaatgc
       61 gcgaagatat tttgcgtttt gccccctttc gaggcgcggt gcgtccggcg gaagaggctg
      121 tgtttcacaa agtgcaaacc gatattttga cgggttcact caacatcagc actgaaatcg
      181 gtgcgctttc gagcgaggtc cagggaaact gatgccacaa tcggttcaac cttcaccgtt
      241 agcggtctcg atcaatccct ggtcgtcaat tgccaaccag ttgcggtttg gactggtggt
      301 gattgtggcg ctggccctgc tggtgacggg cagcttgctg atttactcca gttttcaaac
      361 gctgattcag gaaaccaaca gcttgcagcg cgaacgatca cgggttgcgg ctgaaacgat
      421 tgatgcctat ctcgatgatt tgctgagaaa attaaattac ctggccaggg ttcggggact
      481 cacggattta cccaccgaga ccaaacaaac gatgcttgag gggcttgccc ggcataatca
      541 ggcgtatgag ttgattgcca tcctggatcg cgaaggaaac gtccagtcat cagtgagtcc
      601 ctatggtggt gccaaaccag aacccatgcg gaattcctcg ctgtttatcc gcacgttcct
      661 caagcacgaa gattttgtgg ccgatgtcac gattgaccca gacctcaacc agcccgtgac
      721 gacgtttgcg gtgccgattc gcaatgccca ggatagcgtg gatggggtat tactggcccg
      781 gatcaacctc aaatttctga actttgttac ctctcagacc gaagttggcc gcaccggcta
      841 tacctatgtg gttgacgctc gaaaccggtt gatttcacgc aaaggcacca ccgggcaaaa
      901 gattcacctg gaagacgttt cacggctttt ctttctccag gatctcacca ttaccaaagc
      961 cagatcactg gctgtctatc atggcctcta tgacgctgag gtcctgggcg ccgtggcccc
     1021 ggtccgcaat gtcaactggc tggtggtggt tgaactcccc accgccgaag cctatgcccc
     1081 ggttcgacgg caaattctca ccatggggct cagtttactg gtcgcagtga tcggtgccac
     1141 cggggttggg ttcctgtttg cccagcgcgt cgtggtgcct tttcaacgcc tgacccatgc
     1201 tgcccagcga atccgggatg gaaacctgga ggtgcaggtt cacattcctg accgcaatga
     1261 actgggggtg cttggccagg catttaatac catggccgcg caattacagg cgtcatttca
     1321 ggcactggaa caaatgaacg aagatctcga agcgcgtgtg gttttgcgaa ctgccgaact
     1381 ccaggctgcc tttcaggaaa ttgaaaccct caaccagcaa ctcaaggccg aaaatctccg
     1441 catgggcgcc gaacttgaag tcacgcggca actacaacaa atgcttttac ccagggaggc
     1501 cgagatccag actattgggg acctcgacat cgccgggttt atggaaccgg cggatgaagt
     1561 tgggggcgat tattatgatg tcctgcaaca caacggacgg atcaagatcg ggattggcga
     1621 tgtcaccggg catgggctgg aaagcggcgt ggtcatgatg atggcccaaa cagcggtgcg
     1681 aaccctgctg gtcaacaatg aaaccgaccc ggtgaaattt atgaacacca ttaaccgggt
     1741 gatttatgaa aatactcacc ggatgcgaag cgcccgcaac atgacgtttg cgctcctgga
     1801 atatcaagcc ggggtgtttc gactcagcgg ccagcacgaa gatgtgcttg tggtgcgcca
     1861 ttctgggcag gttgaaaaaa ttgacacctt tgaacttggg tttccgcttg gaatcgaacc
     1921 ggatatttca ccctttgtgg cacaacttga tattgaactg gcaggcggag acgtgctggt
     1981 gatgtatacc gacggcatta cagaggcggt caattccgac caggaacagt acggaataga
     2041 ccggctgtgt gccgtcctga tagctcatcg cgaggcttcg gcagttgaac tccagcagca
     2101 ggtaattgct gatgttaaag cctttatcgg ctcacagaag atcttcgacg atctcaccct
     2161 gctgatcatg aagaaaaagt cagccgacct gaacacctcg cacctgtgag cggattgccg
     2221 gagcccaact ggaatgaaag gcttggcggg cctgtcagat atatgctaaa gcaataacag
     2281 acagtaatca gattttcccc aggctacttt tcccaatgct aataccactt agggttcagg
     2341 gtatcgggtt catggttcag ggaaatacag ttttttcaat ggattagcct tcctataata
     2401 cgaggctttc attccaaaat ggtataaccc ggttttcttg tcataccatg ttggaaaaga
     2461 tatctctcct aacccctaat ccctaatccc taacccctaa cccctaaccc ttaaccccta
     2521 acccccaaat gtttgcatcg agga
//
LOCUS       DAOQDO010000795         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35617_length_2543_cov_3.817524, whole genome shotgun sequence.
ACCESSION   DAOQDO010000795 DAOQDO010000000
VERSION     DAOQDO010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35617_length_2543_cov_3.817524"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1062
                     /locus_tag="PKZ53_12875"
     CDS             97..1062
                     /locus_tag="PKZ53_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41380.1"
                     /translation="MAVVLIIEDEAVLRASMARGISKLSGIVTIEAGTLTEALTHLDE
                     QPPDLIFSDLDLPDRSGIEILGELGKRGWKIPVVFISAYLKAYAPQIPRHAAVEVRDK
                     PVSLEELRQIVVNKVGVAKQQVADPPFSAVDFVQLACLGRHSVTIDMSQNGTPVGNIQ
                     VFQGTVWNAHDPLGHGEDAFRRLLFGREFRVTCRTLIESPQERNIHLGWETLLMEAAR
                     IHDEMKEAGALQQELDDLEQATLEPCETVPESVPPPQALSTEEAFETAWETGINALLN
                     KEYQAALRAFLLANQIQPGNRRVEANVKRLHEMGIVVESPPALTS"
     gene            1087..1905
                     /locus_tag="PKZ53_12880"
     CDS             1087..1905
                     /locus_tag="PKZ53_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ41381.1"
                     /translation="MSRRICIASPKGGVGKTTIALNLAVAMAERGMSTLLVDLDPQGG
                     IGHSLGKGDAELSGLADLLMGQATPAQAVIQTKMPKLRMLPRGKLDPVDAGEYEQALF
                     RPGVLEDALAKVEDAVDITLFDTPSGLGLITRAALANADFVLVPFQTEALALRSISQI
                     LRVIEHVQTNENPKLQLIGLVLTLVEKHQPNALSILSEAWGNFSGVLDTIIPRVDLFA
                     EASQKGLPVGFLAGPISPEARRFEFLAAELDYLMKRLSLMSEVTHVERQQRELL"
     gene            1874..2452
                     /locus_tag="PKZ53_12885"
     CDS             1874..2452
                     /locus_tag="PKZ53_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41382.1"
                     /translation="MSNASNVNCSDLEKARQISHKLSLESRLALPPPRPQPTPPNYIR
                     FHLENVAGQSAKAEVAPPATPVLPPLPPLPDDLYGKETWERLLQWCREAVNGSGAFLI
                     DKEGFVVANHGLITPEKAQEIGARLTIALDQAQQLPDLPETSPIVAFEQATGWITGFS
                     ITIGQLGTLQLGILGPVVAPRMVVDQVRKALG"
BASE COUNT          617 a          657 c          677 g          592 t
ORIGIN      
        1 gatgtcttca gccccaagcc cgatgtcttc agccccaagc ccgatgtctt caacctatat
       61 cttcattcca atcccctcca gtctgaggag tatgttatgg cagttgtatt gatcatcgaa
      121 gacgaagcgg tcctgagagc gtcaatggcc cgagggatca gcaaactatc cgggattgtc
      181 accattgaag ctggcacctt gaccgaggca ttgacccatc tcgacgaaca accgcccgat
      241 ttgatttttt cagaccttga tttacctgat cggtcaggca ttgagatcct gggggagttg
      301 ggaaaacgag gatggaaaat tccggtggtc tttatttcgg cctacttgaa ggcatatgcc
      361 ccccaaatcc cccggcatgc ggcagtcgaa gtccgtgata aacctgtcag cctggaagaa
      421 ctccgccaga ttgtggtcaa taaagttgga gtcgcgaaac agcaagtcgc cgaccctcca
      481 ttttcagccg ttgattttgt ccaactcgcc tgtctggggc gacattcagt cacgattgat
      541 atgtcccaaa acggcacccc ggtgggcaac attcaggttt ttcagggtac ggtgtggaat
      601 gcccatgacc cgcttggcca tggcgaagat gccttccgcc ggttgctttt tggtcgagag
      661 tttcgggtga cttgccggac gctgattgaa tcgccccagg aacgcaatat ccatcttggc
      721 tgggaaacgt tgctgatgga agccgcccgg atccacgacg aaatgaaaga agccggtgcg
      781 ttacagcagg aattagacga cctggaacag gccacgcttg aaccctgcga aacggttcct
      841 gaatcagttc ccccgcctca agctttatca accgaggagg cgtttgaaac ggcctgggaa
      901 accgggatca atgcgcttct caataaagaa tatcaagccg cactccgagc ttttcttctg
      961 gcaaatcaaa ttcaacctgg gaatcgccgg gtggaagcca atgtgaaacg cctccatgaa
     1021 atgggaatcg tggtggagtc cccacccgcc ctcaccagtt aaacaatcca tagaggagaa
     1081 tcagtaatgt cacgtcgaat ttgtatcgca agtcccaagg gcggcgtcgg gaaaacgacg
     1141 attgccctca acctcgcggt tgcaatggcc gagcgaggaa tgagcacact gctcgttgat
     1201 ctggatccac aaggtggaat cgggcattca ttgggaaaag gagacgctga gttatcaggg
     1261 ctggctgatc tgttgatggg ccaggcaaca ccggcccagg ccgtgatcca aaccaaaatg
     1321 cccaaactgc gcatgctccc ccgtggaaaa ctcgacccag ttgatgctgg tgaatatgaa
     1381 caggccttgt ttcgtcccgg tgttttagaa gatgcgctgg cgaaagttga agatgcggtg
     1441 gatatcaccc tgtttgatac gccctcgggt ctgggcctga ttacccgggc ggcattggcc
     1501 aatgcggact ttgtcctggt gccgtttcaa actgaagctc tcgccctgcg ctcaatctcc
     1561 caaattttgc gggtcattga acacgttcaa accaatgaaa atccaaaatt gcagttgatt
     1621 ggactggtgt tgacgctggt tgaaaagcat cagccaaatg cactctccat tttgagcgag
     1681 gcctggggaa atttttctgg tgtcctcgat acgatcattc ctcgcgttga tttgtttgcc
     1741 gaagccagcc agaaagggct tccggttggg tttctcgctg gtccgatttc gcctgaggcc
     1801 cgtcgttttg aattccttgc cgctgaactt gattatttga tgaagcggct cagcctgatg
     1861 agtgaggtga cccatgtcga acgccagcaa cgtgaattgc tctgatttgg aaaaagcgcg
     1921 gcaaatcagt cacaaactaa gccttgaatc acggttggcg ctaccgccac cccggccaca
     1981 acccacacca ccaaactata ttcgctttca tttggaaaac gttgctggcc aatcggccaa
     2041 agctgaagtg gcaccgccgg ccacgccagt tctcccacct ctcccaccat tgcccgatga
     2101 tttgtatggg aaggaaacct gggaaaggct cctccagtgg tgtcgtgaag ctgtcaacgg
     2161 ttctggcgct ttccttattg ataaagaagg gtttgtggtt gctaaccacg gtttgatcac
     2221 ccctgagaaa gctcaggaaa tcggtgccag attgaccatt gccctcgatc aggcccaaca
     2281 attaccggac cttcccgaaa cgtctccgat tgtggccttt gaacaggcaa ccggctggat
     2341 tacggggttc agcatcacga ttggccagct tgggacctta caacttggaa tcctcgggcc
     2401 agttgttgct ccccggatgg tggtggatca ggttcgtaag gcattgggct gaaaaattca
     2461 gggctgaaga agccaggctg gagaatgaag aatgaagaat tgagaatgaa gaatgaagaa
     2521 tgaagaattg agaatgaaga atg
//
LOCUS       DAOQDO010000796         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35620_length_2543_cov_3.674035, whole genome shotgun sequence.
ACCESSION   DAOQDO010000796 DAOQDO010000000
VERSION     DAOQDO010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35620_length_2543_cov_3.674035"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..690)
                     /locus_tag="PKZ53_12890"
     CDS             complement(115..690)
                     /locus_tag="PKZ53_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41383.1"
                     /translation="MKRHLVNPAIDWRIQWLGVVPTLFFLVHFGFHWKSGTPGNGLWM
                     CNASSAALGIGMMCRIPLLIRVATIWLIPGLPLWIYDATRYGMGPATTILEHVGGVVV
                     GLYALHHIRAYRWMWLHGIGWFLLIQQAGRWLTDPKLNVNTAHFPHASVAKSFPVYWQ
                     YWLASMGVVVVSMWLLSWALNRIFPEQQNEE"
     gene            1060..2433
                     /locus_tag="PKZ53_12895"
     CDS             1060..2433
                     /locus_tag="PKZ53_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNJ41384.1"
                     /translation="MALLSLPPGPGPISSDDHVALFRQGPLSFLHDLAQQYGDIVYFK
                     RGTENVFLVNNPQYINNVLVTHHRNFTKGRGLQRAKHLVGEGLLTSETELHRRQRRLA
                     QPAFHRERVAGYAAVMVEKAIRMRERWQPNQVVDMSIDMTAITLEIVTQTLFGTNIEA
                     ETAQISQACADAMAVIKAMNVVDLRRMIGPNIEDQSEYHAQEVQFEEIKNQLDSVISA
                     IIHQRRQSTIDHGDLLSMLLLAQDVEGDETGMTDEQLRDEIVTLFLAGHETTANALTW
                     TWYLLAQHPEIEAQVHAELDRVLGNRVPSARDYPHLSLLNMVFAEAMRLYPPVWMIDR
                     RNLEEYQIGDYTLPPQSVVVMSQWVTHHDPRFFPDPERFDPFRWTPEAKEQRPKFSYF
                     PFGGGPRQCIGEGFAWMEGVLLLATFAQHWKFKLVPGHPIEPIPMMTLRPRFGMPMQL
                     ERRNTQG"
BASE COUNT          628 a          708 c          601 g          606 t
ORIGIN      
        1 gttcttggtt cttggttctt ggttcttggt tcttggttct tggttcttgg aaagtcttga
       61 ttcctaagca ccaagcacca aggacaaagc actaaacact caattcttca ttcttcattc
      121 ttcattctgc tgttcaggga atatccgatt gagcgcccaa ctgagcaacc acatactgac
      181 gacaacaacg cccatcgaag ccagccagta ctgccagtac accggaaatg atttggccac
      241 tgaagcatgc gggaaatgcg ccgtgttgac attgagcttg gggtcggtca gccagcgccc
      301 agcctgctgg atgagcaaaa accagccgat gccgtgcagc cacatccagc gataggcccg
      361 aatatggtgt aaggcataga ggccaaccac aacaccgccg acgtgctcca ggatggtcgt
      421 tgccggtccc attccgtacc gggtggcatc ataaatccac aacggaagcc ccggaatcag
      481 ccagattgtg gccactcgaa tcaagagtgg aatccgacac atcatgccaa tgccaagtgc
      541 agccgatgac gcattgcaca tccagagtcc atttcccggc gttccagatt tccaatgaaa
      601 cccaaaatgg accaggaaaa acagggtcgg aaccacacct aaccattgta tccgccaatc
      661 aatcgccggg ttgaccagat gccgtttcat catgaatttc ccctcaattc ccaggtaatc
      721 aagccgttcg gccaatgaat aaataccaac aatttaatcc cgctgcaaaa ttttattgaa
      781 tgaactaaat atttctttta cagatactta aaaaagcttt aacatctgga tttctgacat
      841 caatactgag caggattttc ccgaaaccgt ctctcaatag acaactccag tcaaagatgt
      901 cataattcca gtttgtttgc caaaatctga ttcgcgtctc tacatctttt tcattcgcgc
      961 ttcaattttc tttccaccat cttaaccagt cgagcttcct ggtgtcatct gacctgaggt
     1021 gtatcaccaa ttgggatttt tgctgttagc cggatgtcta tggccttact gagtctccca
     1081 ccgggcccag gcccaatttc atctgacgac cacgtggcgt tgtttcgcca ggggccgctc
     1141 agctttttgc acgatctggc tcagcaatac ggagacatcg tctacttcaa gcgcggcacc
     1201 gaaaacgtgt ttctcgtcaa caaccctcaa tatatcaaca acgtgctggt tactcatcat
     1261 cgtaacttca caaaaggcag agggttacag cgagccaaac atctggttgg ggaaggcttg
     1321 ctcaccagcg agactgagct tcatcgccgt cagcgccgac tggctcaacc agcttttcac
     1381 cgcgaacggg tggccgggta tgcagcggtg atggttgaaa aagcgatccg catgcgcgaa
     1441 cgctggcagc ccaatcaggt ggttgacatg tcaattgaca tgacggccat caccctcgaa
     1501 attgtgacgc agaccctgtt tggaaccaat atcgaagctg agacagccca gatcagccag
     1561 gcgtgcgctg atgccatggc ggtgattaaa gccatgaatg tcgtggattt acgccgcatg
     1621 atcggaccga atattgaaga tcagagcgaa tatcacgcgc aggaagtcca gtttgaagaa
     1681 attaaaaacc agcttgattc ggtgatttca gccatcatcc atcagcgccg ccagagcaca
     1741 attgaccatg gtgacttgtt gtcaatgttg ttgctggccc aggatgtcga aggtgacgaa
     1801 accgggatga ctgacgaaca gctccgtgat gaaatcgtca cgctgttttt ggccgggcat
     1861 gaaaccactg ccaacgccct cacctggacg tggtatttgc tggcacaaca ccccgaaatt
     1921 gaagcccagg tccacgctga gctggatcga gtgctgggca accgcgtccc ctctgcccgt
     1981 gattacccgc acctgtcgtt gctcaatatg gtctttgccg aagccatgcg actttatcct
     2041 ccagtgtgga tgattgatcg ccgcaacctc gaagagtatc agataggtga ctataccctg
     2101 ccgccccaat cggttgtggt catgagtcag tgggtcaccc accacgatcc acgatttttc
     2161 ccggatcccg aacgctttga cccgttccgc tggaccccgg aagccaaaga acaacgacca
     2221 aaattttctt atttcccatt tggaggcggc ccccggcaat gtattggtga aggttttgcc
     2281 tggatggaag gcgtcctcct gctggccacc tttgcccagc actggaaatt caaactggtt
     2341 cctgggcatc cgattgagcc gattcccatg atgacgctcc gccccagatt cggaatgcca
     2401 atgcaactgg aaagacgcaa tacccagggc tgagggcttg gggcttgggg ctgaagattt
     2461 tcgggctgaa gaactggttt tctttcatcc ctcatccctc accccttcga ttgctccagg
     2521 ccctcagccc gaaaatcttc agc
//
LOCUS       DAOQDO010000797         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35638_length_2543_cov_2.160370, whole genome shotgun sequence.
ACCESSION   DAOQDO010000797 DAOQDO010000000
VERSION     DAOQDO010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35638_length_2543_cov_2.160370"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKZ53_12900"
     CDS             <1..572
                     /locus_tag="PKZ53_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ41385.1"
                     /translation="EALVYAAQLHKNQLRKGSHTPYVAHLLAVAAIVIEHGGDENQAI
                     AALLHDAIEDQGGDTTRQEIRRRFGDDVTELVDGCTDAETIPKPPWRARKEAYIEHLH
                     TASARIRLVSAADKLHNARAILTDYRFQGEEVWEYFRGGKEGTLWYYRTLLTVFQAGE
                     PNRLIDELEHTVRELEALVGQNEELRMKN"
     gene            complement(813..1310)
                     /locus_tag="PKZ53_12905"
     CDS             complement(813..1310)
                     /locus_tag="PKZ53_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNJ41386.1"
                     /translation="MAQQNTFDITSKIDHTEVNNAVQQALKEIGQRFDFKGSKCDIQL
                     EPKEIILEADDAYKLKSLTEVLEARLVKRNVPLKGLSYGKIEPAAGDTVRQRIEIQQG
                     IPTEKCKDIVKFIKETKLKVQATINGDFVRVAGKDRDILQEVIGRLKTQDFGIDMQFT
                     NYRST"
     gene            complement(1499..>2543)
                     /gene="gcvPA"
                     /locus_tag="PKZ53_12910"
     CDS             complement(1499..>2543)
                     /gene="gcvPA"
                     /locus_tag="PKZ53_12910"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNJ41387.1"
                     /translation="QGTVHSIFEFQTMICQLTGMDVANASMYDGSTALAEAMMMAVRV
                     TGRRKVVCAGTVHPEYLQVIKTYAYNSGITVETIGWGDTGTVEMETLGVDKETAAVIV
                     QSPNFFGSIEDLSQLATAARAAGALLAVCVTEAISLGILKTPGESGADIVVGEGQSLG
                     LAPNFGGPFVGFFAAREKYQRQMPGRLVGQAFDKDGNRAFTITMATREQHIRREKATS
                     NICTNEALCALVASVFLATLGRRGMQELAIRNAQKTAYALSEICKVDGYSRTFSAPVF
                     NEFAIRTPIPAKQVVDLLLPHNILAGLPLSHYYPDRTHDLLVCVTENTSRAAIDQLVS
                     ALASLSQKAAAAS"
BASE COUNT          616 a          649 c          601 g          677 t
ORIGIN      
        1 aagaagccct ggtgtatgca gcccagcttc acaaaaatca gcttcgtaaa ggttcacata
       61 caccctatgt cgcgcattta ctggcagttg ctgctattgt catcgaacat ggcggcgatg
      121 aaaaccaggc cattgcggcc ctgttacacg acgctatcga agaccaggga ggcgacacca
      181 cccggcagga aatccgccgg cgctttggtg atgacgttac ggaactggtg gatggctgta
      241 ctgatgccga gacgatcccc aaaccaccct ggcgtgcccg gaaagaggcc tatatcgagc
      301 acctgcatac agcttcggcc cgtattcggc tggtctcagc cgccgacaaa ctgcacaatg
      361 cgcgtgcgat cctgaccgat tatcggtttc aaggtgaaga ggtatgggaa tatttccgag
      421 gcggcaaaga ggggacgctg tggtactacc ggacgctcct cacagtcttt caagctggtg
      481 aacccaatcg cctgattgat gaattggaac atacagtccg tgagcttgag gcactggttg
      541 gtcagaatga agaattgaga atgaagaatt gagaatgaag aatgaagaaa aaattggttc
      601 ttggttcttg gttcttgatc cttggttctt ggttcttggt tcttggtcct tggttcttgg
      661 ttcttggttc ttggaaggta ttcgatccta agcactaagc tccaagtacc agatacaggt
      721 ttgcctcttc attcttcatt cttcattctt cattcttcat tcctaattct tcattcttca
      781 ttcttcattc ttcattcttc attcttcatt cactaagtgg agcgataatt ggtgaactgc
      841 atatcaatcc caaagtcctg ggttttcagc cgaccaatca cctcctgcaa aatatcgcga
      901 tccttgccgg ccacccggac aaagtctccg ttgattgtgg cctgaacctt gagcttggtc
      961 tctttgataa atttcacgat atctttgcat ttttcagttg gaatgccctg ctgaatctca
     1021 atccgctggc gaacggtgtc gccggcggct ggctcaattt ttccatagct cagccccttg
     1081 agcggaacat tgcgttttac cagtcgggct tccagaactt cagtcaggct tttgagttta
     1141 taggcatcat ccgcttcgag gataatttcc ttgggttcga gttgaatgtc acatttgctc
     1201 cctttgaaat caaagcgttg accgatttct ttgagggcct gttgaaccgc gttattgacc
     1261 tcagtgtggt caatttttga agtgatatca aaagtatttt gttgcgccat atcttgagaa
     1321 agaaaacggg cggtcactca gccagtggtg aagtgagcgc ccggaccctg gatgtgaata
     1381 ccaattttaa agagtaaatt atgagttatg aaccagcaaa ttacttcata ttcaatgaga
     1441 tagcctgttc atgattcata atttctatca gttgacagtt tcaacggtgg ttttttcctt
     1501 atgaagctgc cgccgctttc tgggaaagag aggccagcgc cgaaaccagt tgatcaatcg
     1561 ctgcccgact ggtgttttca gtcacacaga ccagcaagtc atgcgttcga tccgggtagt
     1621 agtgcgaaag cggtaatcca gccaggatat tgtgtggcaa taacagatca accacctgtt
     1681 tggctggaat cggagtgcga atcgcaaatt cgttaaaaac gggcgccgaa aatgttcgtg
     1741 aatacccatc cactttgcaa atttcactca aagcgtaggc agttttttgg gcattacgga
     1801 tcgccagttc ctgcattccc cgtcgcccaa gcgtagccag aaaaacactg gcgaccagcg
     1861 cacacaaggc ttcgttggta cagatattgg aagtcgcttt ttcacggcgg atgtgttgtt
     1921 cccgggtggc catggtaatg gtaaatgccc ggttcccatc tttatcaaaa gcctgtccaa
     1981 ccagccgacc tggcatctga cgttgatatt tttcacgggc tgcaaagaat ccgacaaacg
     2041 gaccaccaaa atttggtgcc aaccccagtg attgaccttc acccaccaca atatcagccc
     2101 cggattcgcc tggagttttg agaattccca gactaatggc ctcggtgaca caaactgcca
     2161 gcaaggcacc agccgcacgg gcagccgtcg ccagttgcga caaatcttca atactgccaa
     2221 agaaatttgg agactgcaca atgaccgcgg cggtttcttt atcaaccccc agtgtctcca
     2281 tctcgacggt gccagtgtct ccccagccga tggtctcaac cgtaatccca ctattgtagg
     2341 cataggtttt gatcacctgc aggtattcgg gatggacggt accggcacaa accacttttc
     2401 gccgtccggt gacccgaact gccatcatca tggcttccgc cagtgccgtc gaaccgtcat
     2461 acattgacgc attggccaca tccattcccg tcaactggca aatcatggtt tgaaattcaa
     2521 aaatcgaatg caccgtaccc tgg
//
LOCUS       DAOQDO010000798         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35731_length_2539_cov_2.678341, whole genome shotgun sequence.
ACCESSION   DAOQDO010000798 DAOQDO010000000
VERSION     DAOQDO010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35731_length_2539_cov_2.678341"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKZ53_12915"
     CDS             <1..625
                     /locus_tag="PKZ53_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41388.1"
                     /translation="QATDQLVVYSNTESYPVMACWIFDGRTGKRLFSLPTTNGFSKTF
                     VLSDGRYLQAIPTYQTVVTHKVIHGQVTYILKVQFNLFPADGKKHSSFELALEVKETK
                     SNSFFFCAETKLGEVLCLVNNESGKSQLYKIDCDTGQVTPRMSFEFTSSPVSTSQDEY
                     ITISAAACMLRKEWPRDDVEVPYYHIDPTTWNRRQLYATNFSRSFYY"
     gene            1112..2521
                     /locus_tag="PKZ53_12920"
     CDS             1112..2521
                     /locus_tag="PKZ53_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNJ41389.1"
                     /translation="MKSIARLFQITILACLICGASVAATAQSRDTYVISARAGLVNLV
                     SGTVTYQHAGEMRWRSMASVEELGPGDRVRTSAGGRVEILLNPGSYLRLNENSEFELT
                     NPSFDDLQISLLSGNAIIEMTGTDGSEVFMKVLTPKQTFSIIDDGLYRVNVLPDGISE
                     LLIRKGKAALASDPSKKIKDGRKIIASAAEPTIEKFDKKKDQDMFDMWSGQRAELLAT
                     ANSRLTDNRILTGINAYQAAGNYGFGYRPFYGLWFYDPFLSGYSFFPFYSDWFSPYGF
                     NYTICYGLPWWYYRPLPYGYFPNGYGYCCNGGTGAGTGTGTGTGTGTGTGTTAGNDSK
                     FTVAPPVRTRPTLTGGTPGVTTTKNNKTTLPEISGKPMPGVSGSGASVASTRPTANKQ
                     PKEFPTPAKAISIRTTLNSLADSERGYGGGGGYGGASSTSSGRGGGGGAGRVTAAPSA
                     GSTPRPTSPSVSPGRTRNN"
BASE COUNT          657 a          703 c          578 g          601 t
ORIGIN      
        1 ccaggccacc gatcaactgg ttgtttactc aaatactgaa tcctacccag tcatggcttg
       61 ctggattttt gatggtcgaa ccggaaagcg actcttttca cttcccacaa ccaacggatt
      121 ttcaaaaacc tttgtgctct cggatgggcg atatcttcag gccattccaa cataccaaac
      181 cgtcgtcact cataaggtta tacacggtca agtcacttat attttaaaag tacaatttaa
      241 tctttttcct gccgatggaa aaaaacactc ctccttcgaa ttggccctgg aggtcaaaga
      301 aacaaaatcc aactcatttt tcttttgtgc cgaaaccaaa ttaggagagg tactctgtct
      361 ggtcaataat gaatctggca aatctcaact ctacaaaatc gattgtgata caggacaagt
      421 taccccaaga atgagttttg aatttacctc atcacccgtt tctacgagtc aagatgagta
      481 tattacaata agcgcagcag cctgtatgct gagaaaagaa tggcctcggg atgacgtcga
      541 ggttccctac tatcatattg acccgacaac ctggaatcgc cggcaactat atgccaccaa
      601 cttttccagg tcgttttact actgagagat tcactacacc atatcgtgta gtcccttccg
      661 cagatcgttg ctgttttatc gctcgacctc ttgcccttca ccgaatctat cgtttgttca
      721 gcagggggtt ggatttttcc tgagtcacgt aattcctgcc tctgtactat caacttgcaa
      781 tgggatcccc gtaagagaaa ccggtcaagt gattcaatca aaaaatctta gcgaactact
      841 gactactgac tactgacaac aaactggtat cagcaattga ccgggctccc cttcccaacc
      901 cggaattcca tctgacctga atggcctgct ggcacgaaaa ttgttcgctt tttcaaatgc
      961 ttcgcgtcac aatggttcgc actcacaatt tcgccgaagc acattctctc aatccacttg
     1021 atcaaatttg tcagaaccgc cccccagatc attcagccgc tcagagtgac gtgaggtggt
     1081 tccttccagg aagaaaggta gggtcgcgct tatgaaatca atagctcgac tatttcagat
     1141 tacaatcctt gcgtgtttga tttgcggagc ttccgttgcc gcaactgccc aaagccggga
     1201 cacctatgtg atttccgccc gggctggact ggtaaatctt gtatccggaa cagttaccta
     1261 ccagcacgcg ggtgaaatgc gctggcgctc aatggcctcc gtcgaagaac tcggacccgg
     1321 tgaccgggtg cggacttcgg caggtgggcg ggttgaaatc ctgctcaacc ccggctccta
     1381 tttgcgcctg aacgaaaatt ctgaatttga actaaccaat ccttcatttg acgaccttca
     1441 aatttcactg ctcagcggaa atgcgatcat cgaaatgacc ggcaccgatg gcagcgaagt
     1501 ctttatgaaa gtcctcaccc caaaacaaac cttttccatc attgatgacg gcctttatcg
     1561 ggtcaacgta ctcccggatg gaatcagcga actcctgatc cgaaaaggca aagcagctct
     1621 ggccagcgac ccgtccaaaa aaatcaaaga tggacgaaaa atcattgcca gcgcggcaga
     1681 accaaccatt gaaaaattcg ataagaaaaa agaccaggac atgtttgata tgtggagcgg
     1741 ccagcgggct gaactcctgg ccacggccaa ttcccgcctg accgacaacc ggattctgac
     1801 cggaatcaat gcctatcagg cagccggaaa ctatgggttc ggctatcgtc cgttttatgg
     1861 cctctggttc tatgacccgt tcctgagcgg ctattcgttc ttcccgtttt atagtgactg
     1921 gttttcaccc tatgggttta actacacgat ctgctatggc ctgccgtggt ggtattaccg
     1981 accattgcca tacggatatt tcccgaatgg atacggctac tgctgcaatg gcggaactgg
     2041 tgcaggcacg ggaaccggta cagggaccgg caccggaact ggcactggca ccactgccgg
     2101 aaatgactcg aaattcacgg tcgccccgcc agttcgcacc cggccaaccc tgactggcgg
     2161 aaccccaggc gtcacaacca ccaaaaacaa taaaaccacg ttgcctgaaa tctctgggaa
     2221 gccaatgcca ggtgtaagcg ggagtggggc ttcagtagca tcgacccgcc caacggccaa
     2281 caaacaaccc aaagagttcc ccacaccggc caaggccatc tcgattcgca ccacgctcaa
     2341 tagcctcgct gactcagaac gaggttatgg cggtggtggt ggatatggtg gggcatcaag
     2401 cacttcaagc ggtcgtggcg gtggtggtgg cgcaggtcgc gtcacagccg caccttcagc
     2461 cggaagcacc cctcgcccaa ccagcccatc tgtgagccca gggcggaccc gcaacaatta
     2521 ataccagttt gtagtcagt
//
LOCUS       DAOQDO010000799         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35754_length_2538_cov_2.646798, whole genome shotgun sequence.
ACCESSION   DAOQDO010000799 DAOQDO010000000
VERSION     DAOQDO010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35754_length_2538_cov_2.646798"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1384)
                     /locus_tag="PKZ53_12925"
     CDS             complement(143..1384)
                     /locus_tag="PKZ53_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ41390.1"
                     /translation="MEVRVEPRAEWKQMFYEIWRHERDFFYDPNLHGLNVEKVVKTYE
                     PFLASVNHRTDLNYLFIEMLNQLTVGHMFISGGDVPDVKRVPGGLLGADYTIDQNRYR
                     FAKVYNGENWNPQVKAPLTQPGVNVKAGEYLLAVNGRDLTARENVYAAFESMAGKSVL
                     IKVGPTPDGVGAREVTVVPVGSEAPLRNLAWIEGNRRKVEQLSGGKLGYVYIPDTGFG
                     GYNSFNRYFFSQTNKDGAVIDERFNGGGLLADYIVSYLSRPQLNFIRYRHGADVNVPA
                     GAIYGPKVLLINELAGSGGDALPWYFRKMNVGQMIGKRTWGGLIAAFGAPTLMDGGRV
                     TAPDAALYGLKGEWEVENAGVAPDIEVEFDPAAWRQGRDPQLEKGVEVLLEDLKKNPR
                     PQYPQPKYPNYHQNTNLGGKP"
     gene            complement(1591..>2538)
                     /locus_tag="PKZ53_12930"
     CDS             complement(1591..>2538)
                     /locus_tag="PKZ53_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41391.1"
                     /translation="AAISPTGARAVFEARGEIITVPAEKGDPRNLTNSPGVMERDPSW
                     SPDGKWIAYFSEASGEYALHVSDQKGGEVKRFPLGANPSFYYSPTWSPDSQKIAFYDT
                     ANTLWYLQLDKGTLTKVDTNPFGLRDNVVIPNWSPDSRWIAYVKQLPNLLRAVFIYSL
                     EDNKFRQVTDGMSDARHVNFDKSGKYLYFSASTNIGPSLSFADLSGIAHQVTRSLYLV
                     VLRSDLPSPLAPESSDENDPEAKSDKKPNGDGPKPDGPSGPVPRKSRWSQSGLILTAL
                     NSASWRCPCRRQTMRIWWLGKPTHCLSSKHHRRVQAPLV"
BASE COUNT          516 a          690 c          730 g          602 t
ORIGIN      
        1 gcgccggcgg tgatgtcggc tttgtacgtg atcaaggaga gatctccttt tcaggcagga
       61 tgtttttgtc agtaccacct gcgtgagcgg gtgggttggt acgaactggg cccacccgct
      121 cacgcaggtg gtactgacca gatcagggtt tgccgccgag gttggtgttt tgatggtaat
      181 tcgggtactt cggctgcgga tattgcggac gtgggttttt cttgaggtct tccagcaaga
      241 cttcgacccc tttctcaagc tgtgggtcac ggccctggcg ccaggctgcc gggtcaaatt
      301 cgacttcgat atccggggcc acgcccgcgt tttcaacttc ccactcgcct ttcagtccat
      361 agagagcggc atcgggtgcg gtgacccggc ctccatccat caacgtcggg gcgccaaagg
      421 ccgcaatcaa cccgccccag gtgcgcttgc cgatcatctg accgacgttc atcttgcgga
      481 aataccaggg cagggcatca ccgccgcttc cagccagttc gttgattaac agaactttcg
      541 ggccgtaaat cgctccggcg gggacattga catcggcacc gtgacgatag cgaatgaaat
      601 tgagttgcgg acggcttaaa taactcacga tgtaatcagc cagcaagccg ccaccgttaa
      661 agcgctcgtc aatcacggcg ccgtctttgt tggtttggga aaagaaatac cggttgaaac
      721 tgttataacc accaaacccg gtgtctggaa tgtagacata cccgagcttg ccaccactca
      781 attgttcgac tttgcggcgg ttgccttcga tccaggccag gttgcgcagg ggggcttcgc
      841 tcccaactgg aacgacggtg acttcgcggg caccaacgcc atcgggggtt ggaccgactt
      901 tgatcaggac acttttcccg gccatgcttt caaatgcggc atagacgttc tctctggcgg
      961 tcaggtcgcg accattgacg gccagcaggt attctccggc tttgacattg acgccaggct
     1021 gggtcagtgg cgctttaacc tgcggattcc agttttcgcc gttatagacc tttgcaaagc
     1081 gatagcggtt ctggtcaatc gtgtaatcag cgccaagcaa tcctccgggg acgcgtttca
     1141 catccggcac atcaccgcca gaaatgaaca tatgcccgac ggtcaactgg ttgagcattt
     1201 cgataaagag gtaattgagg tctgtacgat ggttgacgct ggccagaaag ggttcgtagg
     1261 ttttgacaac tttctctaca ttgaggccgt gcaggtttgg gtcataaaag aaatcgcgtt
     1321 cgtgacgcca gatttcataa aacatctgtt tccattcggc acgtggttca acccggactt
     1381 ccatggcggc gacattgagg ctgccttcgc cgggcttgcc ggggccgtca ccaggtcggc
     1441 caccgccgcc gggcattccg gtcggggcaa tcatccattg gggaccggtc tggtacagca
     1501 ttttttcgcc attggccgaa acggcaaagc tcccgacatt ggccagaagg cgttcggttt
     1561 tgcgcttttc caggtcaaac ttgtgaacaa tcaaaccaaa ggcgcctgga cccggcggtg
     1621 gtgcttcgag gacaaacaat gtgttggctt tcccagccac cagatccgca tagtttgacg
     1681 tcggcagggg caacgccagg atgcgctgtt caatgccgtc aaaatcaatc cggactggct
     1741 ccaccggctt tttctcggca ccgggcctga tggcccgtcg ggcttcggtc cgtctccgtt
     1801 tggttttttg tcggatttcg cttccgggtc attttcatcg ctgctttctg gcgccagagg
     1861 ggacggcagg tctgagcgca gcacgaccag atacaggctc cgggtgactt gatgggcaat
     1921 gcctgacaga tcagcaaaac tcagcgatgg gccaatgttg gtgctggctg agaaatagag
     1981 gtatttcccg cttttatcaa aattgacgtg acgggcatcg ctcatgccgt cggtgacctg
     2041 ccgaaatttg ttgtcttcca gcgaatagat aaacaccgcc cgcaacaaat tgggcaattg
     2101 tttgacgtag gcaatccatc ggctatccgg cgaccagttg ggaatcacga cattgtcgcg
     2161 cagcccaaac gggttggtgt caactttggt gagggtgcct ttgtcaagct ggaggtacca
     2221 cagggtattg gccgtgtcat aaaaggcgat tttctggctg tctggcgacc aggttggcga
     2281 gtaataaaac gacgggttgg ccccgagcgg aaatcttttc acttcgccgc ccttttgatc
     2341 actcacgtgg agggcatatt cgccagacgc ttctgaaaaa taggcaatcc attttccgtc
     2401 tggtgaccac gacgggtcgc gttccatcac gccagggctg ttggtcaggt tgcgtgggtc
     2461 acctttttca gccgggacgg taatgatttc acctcgggct tcaaacacgg cacgggcacc
     2521 ggtgggagaa atcgccgc
//
LOCUS       DAOQDO010000800         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35767_length_2537_cov_3.462127, whole genome shotgun sequence.
ACCESSION   DAOQDO010000800 DAOQDO010000000
VERSION     DAOQDO010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35767_length_2537_cov_3.462127"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..683)
                     /locus_tag="PKZ53_12935"
     CDS             complement(48..683)
                     /locus_tag="PKZ53_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3859 domain-containing protein"
                     /protein_id="HNJ41392.1"
                     /translation="MSSRLTQTQLSQVVTEVTRLAHQREELERSTLERAEVERILREL
                     DLPAELLDDAMVELERRELVAQQKRSKNRIVMAVAAVVLVLVLGVGWIFYLQSAALGR
                     VNAEPGQITLGANDAQAVQVINKQANREVFNQVVLRDTPEGKELNLKCKWYDPTGKVY
                     RENSYRTKTIDKSVWPTHCKCQLGSDAASGTWKVELSLEGRVISTTTFKVE"
     gene            complement(708..1727)
                     /locus_tag="PKZ53_12940"
     CDS             complement(708..1727)
                     /locus_tag="PKZ53_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ41393.1"
                     /translation="MSQSNRGADHLSTGQMTGVYAALLAVQLFFGINYYASKVVLSEI
                     PPQAWAGIRVTSAAVVLLLIHSLFVRRYPAREDLRWFVGFALFGIVINQILFVEGLAR
                     TTPTHSAIIGTTIPVMTLIFAVLRREESLRMLKILGVVLSLSGVCWLLGIHRFQFESQ
                     QQFGDALTFLNCASFAFFLVLSRKVVARYDALTSTTLLMVFGAIGVDLVCAKPLFLFF
                     STQVSKVSTSTWWHVAYVIIFPTILTYGLNYWALKRVDASLVAVFIYIQPVVAASLSV
                     LFLGETITFKLVGSTVLVFTGVLLTMQQTQHWVSRWGLAFRNEADGFSDTETGEHSKN
                     PSFSA"
     gene            1836..1911
                     /locus_tag="PKZ53_12945"
     tRNA            1836..1911
                     /locus_tag="PKZ53_12945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1869..1871,aa:Arg,seq:tcg)
     gene            complement(1999..2310)
                     /locus_tag="PKZ53_12950"
     CDS             complement(1999..2310)
                     /locus_tag="PKZ53_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ41394.1"
                     /translation="MQSHSNHQFVASVVGFPNCVAEGQTREEAIAKVKSAIEAQLSQG
                     ELIVVEVVSKSDPSLPNPWLKYMGIFADDPTFDDFLEEVSAYRRQMDTPEIEFPGHVL
                     S"
BASE COUNT          685 a          658 c          582 g          612 t
ORIGIN      
        1 attcttcatt cttcattctc aattcttcat tcttcattct tcattcttca ttctactttg
       61 aaggtggtcg tactgatgac ccggccttcg agtgagagtt caaccttcca ggtccctgag
      121 gcagcatctg aaccaagctg acatttgcag tgtgtcggcc agactgattt atcaatcgtt
      181 ttggtgcggt aggaattctc ccgatagacc ttgcccgtcg gatcatacca tttgcatttc
      241 aggttgagtt ctttgccttc gggagtgtcg cgcaagacaa cctgattgaa cacttcacgg
      301 ttggcctgtt tgttgatgac ctgcaccgcc tgtgcatcat tggcaccaag ggtaatctgg
      361 cctggctcgg cattaacacg cccaagtgcg gcgctttgga gataaaagat ccatccaacg
      421 ccaagcacca gcaccagtac taccgctgca acggccatca caattcgatt tttggagcgt
      481 ttttgctgag cgaccagttc acggcgttcc agctcaacca tggcatcatc gagcaattcg
      541 gctggcaggt ccagctcacg caaaatccgc tcgacttcgg cgcgttccag cgtagagcgt
      601 tccagttcct cacgctgatg cgccagccgg gtaacttcgg taaccacttg cgataactga
      661 gtttgtgtta agcgactcga catagcacct caccaaatgc tttttgatca agctgaaaat
      721 gaagggtttt tcgaatgttc tccggtctca gtgtctgaaa acccgtctgc ctcgttcctg
      781 aatgccaaac cccatcggct cacccagtgc tgtgtctgct gcatcgtcag gagcactccg
      841 gtaaagacca gaactgtact tcccaccagt ttgaacgtga tggtttcgcc caggaagagc
      901 accgacagac ttgcggcaac cactggctga atatagataa acaccgcgac cagtgaagca
      961 tccacgcgtt taagtgccca gtaattgagt ccgtaagtca aaattgttgg gaaaatgatg
     1021 acataggcca cgtgccacca cgtgctggtt gataccttcg acacctgagt tgagaagaaa
     1081 agaaaaagtg gtttagcgca aaccagatca acaccaatgg ccccaaaaac catcaggagc
     1141 gtggtcgaag tcagggcgtc gtacctggcc acgactttgc ggctgagaac cagaaagaag
     1201 gcaaatgaag cacagttcag aaaggttaaa gcatcgccaa attgttgttg tgactcaaat
     1261 tgaaaccgat gaatccccag cagccagcac acaccactca gagaaagcac aactcccagg
     1321 attttcaaca tccgcaagga ttcttcccgg cgaagcaccg caaagatcag ggtcatcact
     1381 ggaatggtcg ttccgatgat cgccgaatga gtcggcgtcg tgcgggcaag tccttcgaca
     1441 aacaagattt gattgatgac aatgccaaaa agcgcaaacc caacaaacca ccggaggtct
     1501 tcgcgtgctg gataccgccg aacaaacagc gaatgaatca gcaacagcac taccgccgcc
     1561 gatgtgaccc gaatgcccgc ccacgcctgc ggaggaattt ccgagaggac gactttcgaa
     1621 gcgtaataat tgatcccaaa aaagagctgc accgccagca gcgcggcata cacgccggtc
     1681 atctggcccg ttgaaaggtg gtcggcgcca cggtttgact gactcacaag atttagttcc
     1741 ttctgaatga agcgaaaaag atgattgaca ggtgatcaat agtacagtag aaacccagtt
     1801 caatcactga ctgttcccaa aaagtcacat catctgcacc cgtagctcaa tggatagagc
     1861 atcagacttc ggatctgagg gttgggagtt cgagtctctc cgggtgcgcc aatcaaatca
     1921 aaagcccagc tttccggctg ggcttttgat ttttaagaca ctgccttata tctttgaaag
     1981 tagtttaatg ctcgtgtttt aagataaaac atggcctggg aattcaattt caggggtatc
     2041 catttgccgt cgataagcgg aaacctcctc caaaaaatca tcgaaagttg ggtcatcagc
     2101 aaatatcccc atgtatttca accaggggtt gggcaaagat gggtcacttt tagaaacaac
     2161 ttcaaccaca atgagttccc cctgggaaag ttgtgcttca atcgccgatt tgactttggc
     2221 aatggcttcc tcacgagttt gtccttctgc gacacagttg ggaaatccaa cgactgaagc
     2281 cacaaattga tgattcgaat gactttgcac aaaaactgga tattacataa tgtaacctct
     2341 tgactcattt ttactgataa caatcaactg ttcacctggg ttgtactctg tcaagcagta
     2401 attctcaagt ctgaggaatt tccttgcatg aaaactattc caaaattgac cgaagccacc
     2461 attcgtgacc gcattgacag ccggagtttt gaacgcgggc aacaatactt tcgtgaaaat
     2521 gccatcttca acactcg
//
LOCUS       DAOQDO010000801         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35860_length_2534_cov_2.755950, whole genome shotgun sequence.
ACCESSION   DAOQDO010000801 DAOQDO010000000
VERSION     DAOQDO010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35860_length_2534_cov_2.755950"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKZ53_12955"
     CDS             <1..431
                     /locus_tag="PKZ53_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41395.1"
                     /translation="EVFWPKNRGAFLYAAKVARNPYACALGPTVTGLQVKPPVVKAGQ
                     SASVSATLDDKQFGTFGVRRPKAQKIQAAEYYIDTPPWAGGTARQCEAADGKFNRPSE
                     LVLATLPVSLSAGKHTIYVRGQDREGNWGPVSAVFVTVEP"
     gene            675..>2534
                     /locus_tag="PKZ53_12960"
     CDS             675..>2534
                     /locus_tag="PKZ53_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ41396.1"
                     /translation="MPSRQSFVGLLLPGMRIFLFSISVIVLFLGVLPRVSAQAVTITS
                     IKPNQTGLGQSPPKIVIKGTGFVSGARVLFNNTQVDATIKPSGRKIVIKQSPQSFFQT
                     SGVILVKVVLPGGQESNTVQLVVGSLNSIVINEPASLAVNTGSTIRIRASVVDQAGNP
                     VPNAPVTFQSETPDVATVDTTGTVMGKASGSATLLLSSSDTTRRVIVAVSQVINTVPS
                     GIFGDGDIKVNAGTVYASDLRNHLMKSAAIGSPLGNLAGSSGAAGDVNGSFAASRFNG
                     PLGIGFGNNVLLLADTANRAVRRLNLNTRQVDTIITLADVRANVSTVTDWGPRGVVQA
                     ADGSMYITDQLNHVLWRARLSGAQVSVNVFAGAIGETGLRDELGQAARFNGPQELEFG
                     SANVLAVSDRLNKVVRLVALPSGLVSTIRSTANRPASPHRGQFESQQTNVAFSDPFGV
                     DLDLAGNIYVADGATVRVVSLQDGNAEVNDLAQPGTFQKAVGVSVSNNTVFVLDSGKG
                     QVLQIGIGAPTIQSATPSEVTINQSVEVTLKGANFLPGTQVQVNGQLVSTVVESASQL
                     RFLLPPQTLGGSLGLTVLTRGGSAQTTINVVGPPAAPSNLNARAISGKQVDLTW"
BASE COUNT          569 a          640 c          710 g          615 t
ORIGIN      
        1 atgaggtttt ttggccaaag aatcgagggg cgttcctcta tgcggccaaa gttgcccgta
       61 atccatacgc ctgtgctttg ggtccaaccg ttactggcct ccaggtcaag cccccggtgg
      121 tgaaggcggg ccagtccgcc agtgtgagtg ccaccctgga tgacaaacag tttggcactt
      181 ttggggtccg tcgtcccaaa gcccaaaaaa tacaggctgc cgaatattat attgataccc
      241 cgccctgggc tggcggtaca gcgcggcaat gtgaggcggc agatggaaaa ttcaatcgtc
      301 cgtcagaact ggttttagct acattaccag tcagtttaag tgctggaaaa cacaccattt
      361 atgttcgtgg gcaggaccgg gaagggaatt gggggccggt ttcggctgtc tttgtcaccg
      421 ttgaaccctg atagctcaca cggaagcagg ccaaatcgca ggttgatcac caccggggct
      481 gggagtcgtt tcacgaggaa acctcccggc cctttggtgt atgagcctgc aggctcgcat
      541 tccagatgaa aaatattcaa attcccgaaa gactttaaat ttccagactg gagcattttg
      601 gggttgttct ggtagagtag acgctgcttc atccaaggaa atcccaaaca ttctcataat
      661 caccgaggtg agctatgccc tcacgccagt cgtttgtcgg actcttactg cctgggatgc
      721 ggatttttct cttcagcatc agcgtcattg tactgtttct aggcgtgttg ccacgggttt
      781 cagctcaggc cgttacgatt acctcaatca agccaaatca aactggatta ggtcaatccc
      841 cacccaaaat cgtgatcaaa ggaactggat ttgtctcagg ggccagggtt ttgtttaata
      901 acacccaggt ggacgctacc atcaagccca gtggccgcaa aattgtgatc aagcagtcac
      961 ctcagagctt tttccagacg agcggggtta ttttggtgaa agttgtttta ccaggggggc
     1021 aggaatcaaa cacggtccaa ctggtggtgg gtagtctcaa ttcgattgtg atcaatgaac
     1081 cggcgagtct ggctgtcaat actggaagca caattcgaat tcgagccagc gtggtggatc
     1141 aggccggaaa tccagtgcca aatgcgccag ttacttttca aagtgaaacc ccggatgtgg
     1201 caactgttga caccactgga accgtgatgg ggaaggcgag tggctcagcc acgcttctgc
     1261 taagttcgag cgataccacg cgacgggtga tcgttgctgt cagtcaggtc atcaatactg
     1321 tgccttctgg aatttttgga gacggggata ttaaggtcaa tgccggtacg gtgtatgcct
     1381 ctgacctgcg caatcatttg atgaaatccg cagcgattgg cagtccactt ggaaatctgg
     1441 ctggatcaag cggtgctgct ggggatgtca atggaagttt tgccgccagt cggtttaatg
     1501 gcccgctcgg gattggcttt gggaataatg tgttactgct ggctgatacc gccaaccgtg
     1561 ccgttcgccg gttaaatctc aacacccgtc aggttgacac catcattacc ctggctgatg
     1621 tgcgagccaa tgtttccact gtgactgatt gggggccgcg cggtgtggtg caggctgccg
     1681 atggcagtat gtatattacc gaccagttaa accatgtgtt atggcgagca cggctcagcg
     1741 gggcccaggt ttcggtcaat gtttttgctg gagccatcgg agaaaccggg ctccgtgacg
     1801 aacttggaca ggctgcccgc tttaacggac cacaggaact ggaatttgga tcggccaatg
     1861 tgctggcggt ttctgatcgg ctcaacaaag ttgtgcgact ggtggctttg ccctccgggc
     1921 ttgtttctac catccggtca actgccaatc gcccggcatc cccgcaccgt ggccaatttg
     1981 aatcacaaca aaccaacgtc gccttttccg atccgtttgg ggttgacctt gacctggctg
     2041 gcaatatcta tgtggctgat ggggcgactg tgcgtgtggt ttcacttcag gacggtaatg
     2101 ccgaggtcaa tgatcttgcc cagccaggaa cctttcaaaa agcagttgga gtctctgtgt
     2161 ctaataacac agtgtttgtg cttgactcag ggaaagggca ggtgctgcag attgggatcg
     2221 gggccccgac gattcaatca gcaactccca gcgaggtaac catcaaccag tcggttgaag
     2281 tcacactcaa aggggcaaat ttcctaccgg gaacgcaggt tcaggtcaat gggcaactgg
     2341 tttcaaccgt ggtggagagt gcttcgcagc ttcgatttct cctgccgcca caaacgcttg
     2401 gcggaagcct tggcctgacg gtcttgaccc gaggggggag tgcccaaaca accatcaatg
     2461 tggttggccc gcccgccgca ccatcaaatt tgaacgcccg ggccatttca ggcaaacagg
     2521 ttgatctgac ctgg
//
LOCUS       DAOQDO010000802         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35862_length_2534_cov_2.702299, whole genome shotgun sequence.
ACCESSION   DAOQDO010000802 DAOQDO010000000
VERSION     DAOQDO010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35862_length_2534_cov_2.702299"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKZ53_12965"
     CDS             <1..491
                     /locus_tag="PKZ53_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098853.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNJ41397.1"
                     /translation="LLPDMVISTDIIVGFPGETEEDFQETLSLYDEVGYDLAYMFIYS
                     EREGTPAARHFEDMPQRIKSERIMRLVDKQKDWSLRRYREWIGREVEVLVKGDAFQEE
                     EFVQGHTRGNHVMMVSRQLASQPGIYRVQVTHATPHRLYAEAAPRDSIESLLGSEMVC
                     PS"
     gene            793..1971
                     /locus_tag="PKZ53_12970"
     CDS             793..1971
                     /locus_tag="PKZ53_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNJ41398.1"
                     /translation="MSQPAKELAAAVPALTMLTDEEQLFRSSIADFAKEKVWPKVAEM
                     DRAAVYDRSLIKEFFDLGIMGIEVPTEYGGAGGTFFMSVLAVEEMSRVDGSTGVLVDV
                     QNTLVNNAVLRWGSPEQKKKYFPRLCGDTVASYALSEAGSGSDAFALACRAVDKGDHY
                     SLTGQKLWITNAAEAGFYIVFANINPELGYKGITAFIVERDFPGFSVGKKEDKLGIRA
                     SSTCELILEDCRVPKENVLGEVGKGYKVAIETLNEGRIGIGAQMVGVAAGSLEHGMKY
                     AKERKQFGKRIADFQAIQFQIAELATDVEAARLLVYNAARLRDAGQPFLKEAAMCKYF
                     TSQVAERVTSQVIEIFGGYGYTKEYPVEKYFRDSKIGKIYEGTSFMQLQTIAKLLLTD
                     "
     gene            2098..>2534
                     /locus_tag="PKZ53_12975"
     CDS             2098..>2534
                     /locus_tag="PKZ53_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNJ41399.1"
                     /translation="MQSFSSTDFGAVSSPRSTESKNAQFRWLIIATLLTVVLSLIPYA
                     DILVYPIRLFVTLIHESGHALATVLTFGDVKGLTVSPDESGLTWSQGGFRPLISSAGY
                     LGSMLFGAGLLLLAQRESYARNLLYACGALVFGVTFFFVNGSAS"
BASE COUNT          617 a          612 c          641 g          664 t
ORIGIN      
        1 agctgttacc ggatatggtg atttcgacag atattattgt cggattccct ggtgaaaccg
       61 aagaagattt tcaggaaacg ctctcactct atgatgaagt gggctatgac ctggcctata
      121 tgtttatcta ttccgaacgt gagggcaccc cagcagcccg ccactttgag gatatgcccc
      181 agcgaatcaa atctgaacga atcatgcgac tggtggataa acaaaaagac tggagtctcc
      241 ggcgatatcg tgaatggatt ggccgggaag tcgaagtcct ggtcaaaggc gatgcgttcc
      301 aggaagaaga atttgtccag ggccatacac gcggcaatca tgtgatgatg gtctcgcgtc
      361 agcttgcctc acaacctggg atctatcggg tgcaggtcac ccatgcgacg ccacatcgcc
      421 tgtatgccga agccgcacca cgagactcaa tcgaatcctt gctcggatca gaaatggttt
      481 gccccagcta acaattgaga aatcaggatt caggaggaga caacaggttg gtttgaaaac
      541 acataacctc cctaccgttc tcaaaaggtg gttatttcca aaatcgaacc ccaggttctt
      601 tcatatgaca agaacctggg gtttgttttc tcagaaaact gaactttctt aaaccctgac
      661 ccctgccccc tcgactctca tttgccccat tcctgatatc cccttaaacg tcttcaggct
      721 tttgatactg cgtgatataa atgaaccgac cttccagtgt cttaatttca atcatttcaa
      781 tggagtattc atatgagtca accagcaaaa gagttagctg cagccgttcc cgcacttacc
      841 atgctgaccg atgaagaaca gcttttccgg tcaagtattg ccgactttgc caaagagaaa
      901 gtctggccga aggtggccga aatggaccgg gctgcagtgt atgatcgctc cctgatcaaa
      961 gagttttttg atctgggcat tatgggaatc gaagtcccaa ctgaatacgg tggcgctggc
     1021 ggaacctttt ttatgtcagt gctggcggtt gaagaaatgt ctcgggttga tggttcaacc
     1081 ggcgtgctgg tggatgtcca aaacacgctg gtcaacaatg cggtactccg atggggttca
     1141 cctgagcaaa agaaaaaata tttcccccgt ctgtgtggcg ataccgtcgc gtcctatgcg
     1201 ttgtcagaag ccggttctgg gagcgatgct tttgcccttg cctgccgggc tgtggacaaa
     1261 ggtgaccact atagcctgac cggtcaaaaa ctgtggatta ccaacgcggc tgaagcagga
     1321 ttttatattg tgtttgccaa tatcaatccg gaattgggct ataaaggcat caccgcattt
     1381 attgttgagc gtgatttccc tggattctcg gttggcaaaa aggaagacaa acttggtatc
     1441 cgggcttcgt caacctgtga actgattttg gaagattgcc gtgttccaaa agaaaatgtg
     1501 ttgggtgaag ttggcaaggg ctacaaagtt gcgattgaaa cgctcaatga aggccgaatt
     1561 gggatcggcg cccagatggt gggtgtggct gccgggtcac ttgaacacgg tatgaaatat
     1621 gccaaagaac ggaaacagtt tgggaagcgt attgccgatt tccaagccat tcagttccag
     1681 attgccgaac ttgcaaccga tgtcgaagcc gctcgtttgc tggtctataa cgccgcccgg
     1741 ttacgcgatg ccggccagcc gtttttgaaa gaggcagcga tgtgtaagta cttcacatcc
     1801 caggtggcgg aacgtgtgac ctcgcaggtc attgaaattt ttggtggtta tggctacacc
     1861 aaggaatatc cagtcgagaa atatttccgt gattcaaaga tcggaaaaat ttatgaagga
     1921 acttccttta tgcagctcca gaccatcgcc aaactcttgc tgacggatta gaacttgatg
     1981 cctggttctt ggtgcttggt gcttggttct tcgaagatat tgattcctaa gcaccagtca
     2041 ccaggtacga agcactctcc agtaagaatt ccccgctttt ccccaaagga ggccactgtg
     2101 caatcgtttt catcaactga ctttggagcg gtatcttcgc cgcgttcaac cgaatcgaaa
     2161 aatgctcaat ttcggtggtt gattattgca acactgctga cagtggtctt gagtctgatt
     2221 ccctatgcag acatcctggt ctatccaatt cgactctttg tgacgctcat tcacgaatca
     2281 ggccatgcac tggcaaccgt gctgaccttt ggggacgtaa aagggctcac cgtttcgcca
     2341 gatgaaagtg gactgacctg gtcccaaggt ggattccggc cactcatttc cagtgccggc
     2401 taccttggct caatgctttt tggtgctggc ctcctgctcc tggcccagcg cgagtcctac
     2461 gcccgtaacc ttttatatgc ctgtggcgct cttgtgtttg gagtgacgtt cttttttgtg
     2521 aacggaagcg cctc
//
LOCUS       DAOQDO010000803         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_35902_length_2532_cov_3.205894, whole genome shotgun sequence.
ACCESSION   DAOQDO010000803 DAOQDO010000000
VERSION     DAOQDO010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35902_length_2532_cov_3.205894"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKZ53_12980"
     CDS             complement(<1..672)
                     /locus_tag="PKZ53_12980"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HNJ41400.1"
                     /translation="MNPTASDHSKLALIFSKIFTIERGSPSPFGATLRPNGINFAIFS
                     KNATAVTLVLFVTGEYEPIAQIPLDPHINRTGHVWHIFIRGLDTKIRYGYRMDMEPNP
                     EPDLHKFNPATILVDPCAKALSGASVWGIPYHRIGDADSGWKVRKRRSLIVDNHFDWG
                     HDQPLNIPLSETILYELHVRGFTCHQSSKVQHPGTFVGLTEKIPYLKSLGVTAVELLP
                     IIEFEE"
     gene            922..>2532
                     /locus_tag="PKZ53_12985"
     CDS             922..>2532
                     /locus_tag="PKZ53_12985"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNJ41401.1"
                     /translation="MAKRSLKFNKKPDPIQEAANAERAYNAFMKLLALAPDELHGEFF
                     DLLGDEDRLTNQDQTRERLNGLISQLDLESNRAEIVEVLLELIPVEELVPEVYQKYKP
                     MVVDAGNIILSRLSRERLQTKLVEQLMTPLEASVQERLLTLIARMPTLQKLGQIIARN
                     HNLDPEFRHRLQELENSIRDVTHESIVQRVQEELKSQIESYKIKVSPRILAEASVCAV
                     VPFTWKAPGGERKRGVFKVLKPFIMKYWEEELKTLEALAIHFDRNRASYGLPTVGFKE
                     LLREVRDLLKREVKLNFEQERLRHATRFYSDHEDVRVPELLPMRTKAITGMEFLSGMK
                     VTIAAKKNPHLRDRIAQLVIDKLILSVIFYPQEEALFHADPHAGNLFYEPETDELALF
                     DWGLSCVIDRETRRYIVQLILGFFLRDPKRVFKATCKLTTGKQGPEQQEIIRRKVQEI
                     IDELPLFPLVRIDALTRLLDELILEGIRFPAALLMFRKSLFTIIGVIHDVDSTFHLDW
                     HVTRYLLGQIIGEIPERLRHLPWSRNYPS"
BASE COUNT          659 a          637 c          653 g          583 t
ORIGIN      
        1 ttcttcaaat tcaataatcg gcaggagttc aactgcagtg accccaagtg atttcaggta
       61 gggaattttt tcagtcaatc ccacgaatgt accagggtgt tggaccttcg atgattggtg
      121 acaggtaaaa ccccggacat ggagttcata caaaatggtt tccgagagcg gtatattcag
      181 cggctggtca tgaccccaat caaagtgatt gtcaacaatc aatgaccggc gtttgcggac
      241 tttccatccg ctatcggcat ctccgatccg gtgataagga atgccccaaa cagaggcccc
      301 cgacaacgcc ttagcgcacg gatcaactaa aatcgtggcc gggttgaact tatggaggtc
      361 cggttccgga tttggctcca tatccattcg atacccatac cggatttttg tatccagccc
      421 acgaataaag atatgccaga catggccagt tcgattgata tgcgggtcca gcggaatctg
      481 ggcaattggc tcatattcac cggtcacaaa cagcaccagg gttaccgccg ttgcattttt
      541 tgaaaaaatg gcaaaattga tgccatttgg cctcagagtt gcaccaaaag gggacggact
      601 gccgcgttca atggtgaaaa tctttgaaaa aatcagggca agcttgctgt ggtcagaagc
      661 agttggattc attggtgttt ggtcgtttcg gaagaaaagc ggataaatgg tatggcaaca
      721 ggaacaaatt gcaggtaggg gcgtcactac cttaccagaa gctgggaaag ttgactattt
      781 cccaggttta ctttcgatga aaaacagccg acttgccacc agaaccgttc accaagaagc
      841 acgccatatg gtagagtgag gctacccctt atgatttcca cccacgatca atttccaccc
      901 cactcagcag aggagactca gatggccaaa cgttcgctca aattcaataa gaaaccagac
      961 ccaatccagg aagcggccaa tgccgagcga gcttataatg catttatgaa attgctggca
     1021 ctggcacccg acgagttaca tggggagttt tttgatctgc tgggtgatga agaccgattg
     1081 accaaccagg atcaaacccg ggaacggtta aacgggctga tttcgcagct tgatctggaa
     1141 agcaaccggg cggaaatcgt cgaagtgctg ttggaactga ttccggttga agagttggtc
     1201 cccgaagtgt atcaaaaata caagccgatg gtggttgatg ctggcaacat cattttgagc
     1261 cggttgagcc gcgagcggct gcaaactaag ctggtcgaac agttaatgac cccgctcgaa
     1321 gcatcagtcc aggaacggtt gctgacattg attgcccgga tgcctacgct ccagaaactg
     1381 ggtcagatta ttgcccgcaa ccataacctc gatcctgaat tccggcatcg gttacaggaa
     1441 ctggaaaaca gcatccgcga tgtcacgcat gaatcaatcg tccagcgggt tcaggaagag
     1501 ctgaaatccc agattgagtc ctacaaaatc aaagtcagcc cccggatttt ggccgaagcc
     1561 agtgtctgtg cggtggtgcc ctttacctgg aaagctcctg gaggcgaacg taaacgtggg
     1621 gtgttcaaag tcctcaagcc gttcattatg aagtactggg aagaggagtt gaaaaccctt
     1681 gaagccctgg ccatccactt tgaccgcaat cgggccagct atggtttgcc aacggttggg
     1741 tttaaagaac tgttgcgcga agtccgcgat ttgctcaagc gtgaggtcaa gctcaatttc
     1801 gaacaggaac ggctccgcca tgccacccgg ttttattccg atcatgaaga cgttcgtgtt
     1861 ccggaactgc taccgatgcg caccaaagcc attaccggaa tggaattcct ctcagggatg
     1921 aaagtcacga tagctgcgaa aaagaacccg catctgcgtg accggattgc acagcttgtg
     1981 attgataagc tgatcttatc ggtgattttc tacccgcagg aagaagcgtt gtttcacgct
     2041 gacccgcacg ctggcaattt gttttatgag ccggagacgg atgaactggc gctctttgac
     2101 tggggcttga gttgtgtgat tgaccgcgaa acccgccgct atatcgtgca actcatcctg
     2161 gggtttttcc tccgagatcc caaacgggtc tttaaagcca cgtgcaagct gaccacgggc
     2221 aaacagggac ccgaacaaca ggaaatcatc cggcgcaagg ttcaggaaat cattgatgaa
     2281 ctgccgctct tcccactggt gcgcatcgac gccctgaccc ggttgctgga cgaactgatt
     2341 ttggaaggca tccgatttcc agccgctctg ctgatgttcc gcaagagcct gttcacaatt
     2401 atcggggtga tccacgacgt agactcaacc ttccacctcg actggcatgt gacccgatat
     2461 ctgctcggac aaattattgg agaaatcccc gaacggttgc ggcatctgcc ctggtcgcga
     2521 aactacccca gc
//
LOCUS       DAOQDO010000804         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36096_length_2524_cov_3.935196, whole genome shotgun sequence.
ACCESSION   DAOQDO010000804 DAOQDO010000000
VERSION     DAOQDO010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36096_length_2524_cov_3.935196"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..807)
                     /locus_tag="PKZ53_12990"
     CDS             complement(73..807)
                     /locus_tag="PKZ53_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41402.1"
                     /translation="MKYKGLSTLMRGVVCTSRMQSFMTFTRVALVSLVGLLSGVACQL
                     PSAPPQTPVTPPNQSNQIDLFTCLDQFLPQFSGKLAASTPGKTAFLRQEAEQKLTGAQ
                     VIQPWKPAMPPNTPDDTFKAKIPDMTLGNTHYQCEVTLRTAPGNQTLLGTTFTLASID
                     GQEIAETVDNFSEIVTKHYGEPTLTYEDAEGAEWLFANGKLVMLIERGNDGNTFSGVK
                     SRITFRFDPLSPDDQVPGEITDRDRD"
     gene            1157..2410
                     /locus_tag="PKZ53_12995"
     CDS             1157..2410
                     /locus_tag="PKZ53_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ41403.1"
                     /translation="MNSSTQTMPYREAIMLAYDSIWAHKLRSFLTLLGVILGVMAVVA
                     VASFIEMANKSVLNTVTNDFGANTITLDKYGLITSFDDFIDAQKRNKDITQDDFRALK
                     EGVTLAEDIGIVISQSVETLRVGSQELYGVAINGETPDMANIMVNGTTVEEGRFITDI
                     DNDQRRNVVFIGSKLKETFFPTVNPIGKELRILGEPFEVIGVAKAIGSTFGQERDTYA
                     VIPFQTHLKMFGARQSLTIYFKAPSPEKQEPLQDQIRVIMRARHHLMFSEKDNFGFIS
                     ADALNELWQSLSSLIAAVALAVTSISLVVGGIVIMNIMMVSVTERTREIGIRKSLGAR
                     QKDILLQFLVESSMLSGFGGLLGIILIGTILLIVAQAFSLSVSMPLWAVFLSLFVSLS
                     VGIIFGLYPAYRASRLDPIVALRQE"
BASE COUNT          656 a          594 c          630 g          644 t
ORIGIN      
        1 accaagaacc aagaaccaag aaccaagaac caagaaccaa gaaccaagaa ccaaccacat
       61 tctcaattct ggtcagtctc ggtcccggtc ggtgatttct cctggaacct gatcatcagg
      121 ggaaagtgga tcaaaccgaa acgtgatgcg agacttcaca ccagaaaagg tattgccatc
      181 atttccgcgc tcaatcagca tgaccagctt accgttggca aacagccact ccgcgccttc
      241 ggcgtcttca taggtcagcg tcggttcgcc atagtgtttg gtgacgattt cagaaaaatt
      301 atcaactgtt tcagcaattt cctgaccgtc aatactggca agggtaaagg tggtgccgag
      361 taatgtttga ttccctggcg cggttcgaag ggtgacttcg cactggtaat gggtattccc
      421 aagtgtcata tcagggattt tggctttaaa ggtgtcgtcg ggggtgttgg gaggcatggc
      481 tggtttccag ggctgaatca cctgggcgcc tgtcagtttt tgctctgctt cctgacgtaa
      541 aaacgcggtt ttgccagggg tactggccgc aagttttccg ctaaactgcg gcagaaactg
      601 gtcaaggcag gtaaaaagat caatttggtt tgattggttt ggtggtgtaa ccggggtttg
      661 gggtggggct gatgggagtt gacaggcaac tccactgagg agaccaacca gagacaccag
      721 cgcaaccctg gtgaacgtca taaaactttg cattcgagag gtacacacca caccacgcat
      781 cagagttgat aatcctttgt atttcaaaag ctgaattgac cttgaagacg cgattgacca
      841 tgacttgtgc aaattacact actggctaca aagttgaaaa cgggaaatcg gaatcagcct
      901 gtttgcggtg tcgtccggag tcagagttta cttgaaaaat caatatgtta gaggggttct
      961 cacccggcga tgaaggtgca tccagaaacc aaagataaac ttgaaaccac agccgccttt
     1021 gattcgttct aggcggcaac ttcaaccctt caaatcaaat cacaaaaatt tggatttact
     1081 cagcacctgc gattcagtct gaatcaaaaa caactgaaga cctgtctgga gcagcatttt
     1141 tacgaggtta cttgagatga acagcagtac gcagaccatg ccctatcgcg aagcaattat
     1201 gctggcctat gattcaatct gggcacataa gttgcggagt tttttgacgt tgctcggtgt
     1261 gatcttgggc gtgatggcgg tggtggcggt ggcctcgttt attgagatgg ccaacaaatc
     1321 cgtgttaaat accgtcacca atgattttgg tgccaacacc atcacccttg ataaatatgg
     1381 tctcatcacc agctttgatg attttattga tgcccaaaaa cgcaataaag acatcacaca
     1441 ggacgacttc cgggcactga aagaaggcgt gacactggcc gaggatatcg gcattgtgat
     1501 tagccagtca gttgaaacgc tccgtgttgg ctcccaggaa ctctatggcg ttgccatcaa
     1561 cggtgaaaca ccagatatgg ccaacatcat ggtcaatggc acaaccgtcg aggaaggccg
     1621 atttatcact gacattgaca atgaccagcg gcgcaacgtg gtttttattg gcagcaaact
     1681 caaagaaacg ttctttccca cggtcaaccc gattggcaag gagttgcgaa ttctgggtga
     1741 accatttgaa gtcattggcg ttgccaaggc cattggctcc acgtttggcc aggaacgcga
     1801 tacctacgcc gtgattccat tccaaacgca tttgaagatg tttggcgccc gtcagtcact
     1861 caccatctac tttaaagccc ccagccctga aaagcaggaa ccattacaag atcaaattcg
     1921 ggtgattatg cgtgcccgtc accacctgat gttttctgaa aaagataatt tcggttttat
     1981 cagtgccgat gcgctcaacg aactctggca gagcctgtca agtttgattg ccgccgtcgc
     2041 tctggcggtg acttcaattt cgctggtcgt cggcggcatc gtgattatga acattatgat
     2101 ggtgtcggtg acggaacgaa cccgggaaat tgggattcgc aaatcactgg gcgcccgtca
     2161 aaaggatatt ttgcttcagt ttctggtcga gtcctcaatg ttgtcaggat ttggcggatt
     2221 gctggggatt atcttaattg gaacgattct gctgattgtg gcccaggcat tttcactctc
     2281 ggtttcaatg ccattgtggg ctgttttttt gtcactgttt gtttccctga gcgtcggaat
     2341 tatttttggc ctctatcctg cctatcgagc ctcaagactt gatccaattg tggccctccg
     2401 gcaagagtag tggttggtgc ctggttcttg gtgcttagta cttaggaacc aattctttcc
     2461 aagaaccagg caccaagaac caagaaccaa gaaccaagaa ccaagaacca agaaccaaga
     2521 acca
//
LOCUS       DAOQDO010000805         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36125_length_2523_cov_6.797407, whole genome shotgun sequence.
ACCESSION   DAOQDO010000805 DAOQDO010000000
VERSION     DAOQDO010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36125_length_2523_cov_6.797407"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKZ53_13000"
     CDS             <1..242
                     /locus_tag="PKZ53_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41404.1"
                     /translation="FPATLQVGVRTSEVRTRSFMLEYVVLQPVGEQFEVVADGTTAVV
                     WINIATGKAVALPEELQQAIHHVEAQRTGPVSTET"
     gene            353..862
                     /locus_tag="PKZ53_13005"
     CDS             353..862
                     /locus_tag="PKZ53_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNJ41405.1"
                     /translation="MNTRVLVLNASFEPINICTVRRAVVLILKGVARVEEHTEQFLHS
                     PSAVVKAPSVIRLLEYIHIPFERKSLSRRNVLLRDHNRCQYCGHIFPPIELTLDHVVP
                     RSRGGASTWDNLVACCRKCNNRKGSRTPEEASMRLLKKPQSFNLHVNRQIIRYLGSTD
                     EAWRKYLYF"
     gene            1116..1616
                     /locus_tag="PKZ53_13010"
     CDS             1116..1616
                     /locus_tag="PKZ53_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNJ41406.1"
                     /translation="MSLIFSICVGIALSAACGFRVFVPMLVMSIAAKSGHLGLNPEFQ
                     WIGTWYALTAFAVATGFEIAGYYIPWVDNMLDWIMTPVAVIAGVLASASLMVDASPFF
                     KWSVAIIAGGGAAGAIQGTTVVLRGASTATTAGLGNPALATVEWVLSTIVSALAVLIP
                     MAMLAF"
     gene            complement(1618..>2523)
                     /locus_tag="PKZ53_13015"
     CDS             complement(1618..>2523)
                     /locus_tag="PKZ53_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ41407.1"
                     /translation="IQIQIRPAPWQTIWAYAVYTFVLASTIYLSFRLYTRALEHRNQM
                     LEAKVEARTRDLQEANLKLKELDQLKANFTATLVHDLKSPLTVVNTTLELIKDDEDLN
                     MGPLKPLVEAAERNVQKILNLVNEVLDFYRADSTEKKYKFQVMSPVQFLNDCAESARL
                     TAAPLGITFEKKIPTSLPLLCADFEQLDRVFSNLLSNSIKFTPSGGTICVEVTIIQGK
                     GVETGLTLLAVSITDTGEGIPAEDIPYLFEPYQQAQSNKKRAGVGLGLAIVKRIVAAH
                     GGNISVRSQLGVGSTFTVVLPTSPR"
BASE COUNT          603 a          612 c          675 g          633 t
ORIGIN      
        1 ggttcccggc gaccttgcag gttggtgttc gcacatctga ggtccgcacg cgcagcttta
       61 tgctcgaata tgtggttttg caaccagtcg gtgaacagtt tgaggtcgtc gccgacggca
      121 ccaccgccgt agtctggatt aacatcgcca ctggaaaagc cgtcgcactc cctgaagaat
      181 tgcagcaggc aatacaccac gtagaagccc aacgcacagg ccccgtctca accgaaacct
      241 gaatcaagaa ttgagttccg gctgattttt caagaccaca agacgattca tcacccgaat
      301 gattcagttc tgatcaggtg tcaaaagaac aacactcttg aacggaggtg gcatgaacac
      361 tcgtgttttg gttctgaacg cgtctttcga gccgattaat atttgcacgg ttcgaagagc
      421 cgtggtctta attctcaaag gggttgcccg ggttgaagaa cacactgaac agtttttaca
      481 ttcgccatca gcagtcgtga aagcaccctc agtgatccgc ctgctggaat atattcacat
      541 tccatttgaa cgaaaaagcc tgtcccgccg caatgttctg ctgcgcgacc ataaccgatg
      601 ccagtattgc ggccatatct tcccgcccat cgaattgacc cttgatcatg tggtgccgcg
      661 ttcccgaggc ggcgctagca cctgggataa cctggtggct tgctgtcgaa agtgcaataa
      721 ccgcaaggga agtcggacac cagaggaggc ttcgatgagg cttctgaaaa aaccgcagtc
      781 gtttaacctg catgtcaatc gtcagattat ccgctatctg gggagtaccg acgaagcctg
      841 gcgcaagtac ctctattttt gaatgaagaa tgaagaatga agaatgaaga atgaagaatg
      901 aagaatgaag aatgaagaaa acatcgggtt ccgggttccg ggttccgggt tccgggttcc
      961 gggttccggg ttccgggttt tcgaaaccaa gaaccaagaa ccaagcacca agaaccaaga
     1021 accaagaacc aagaaccaag caccaagcac caagcaccag gaaccaagta ccaagcacca
     1081 ggaaccaagc accaaacccc agaaaccaat cccttatgag ccttatcttc agcatctgtg
     1141 ttggaattgc attaagcgcc gcctgcggtt ttcgggtatt tgttccgatg ctggtgatga
     1201 gcattgcggc caaaagcggt catttagggt taaatcccga gtttcaatgg attgggacct
     1261 ggtatgccct gactgcattt gccgtggcca ctggatttga aattgccggc tactatattc
     1321 cctgggttga caatatgttg gactggatta tgacgccagt tgccgtcatt gccggggttt
     1381 tggcgagtgc ctcgttgatg gttgatgctt cgccgttttt caagtggtca gtggcgatta
     1441 tcgctggcgg tggtgctgct ggtgccattc agggcacgac cgttgtgctg cgtggtgctt
     1501 cgacggcaac cacggctggt ctgggaaatc ccgccctggc gacggttgaa tgggtgctct
     1561 caaccattgt gtcagctctg gcagttctga ttccaatggc catgctggcg ttttaggtta
     1621 tctgggtgaa gtcggtagaa ccactgtaaa ggtacttcct acgccaagct ggctacgaac
     1681 cgaaatattt ccgccgtggg cggcgacgat gcgcttcacg atagccagtc cgaggccgac
     1741 cccggcgcgt tttttgttgg attgggcttg ctggtatggt tcaaacaaat agggaatatc
     1801 ttcagcggga atgccttcgc ccgtgtccgt gatcgaaact gccagcaggg ttaacccggt
     1861 ttcaacccct tttccctgga taatggtgac ttcgacgcag atggttccac cagatggggt
     1921 aaattttatt gaattggaca gcaggtttga aaagacccga tccagttgtt caaagtcagc
     1981 acataggagt ggcagactgg tcgggatttt tttctcgaag gtgataccca gtggtgccgc
     2041 ggtcaatcgg gctgattcgg cacagtcatt gagaaattga actggcgaca tgacctggaa
     2101 tttgtatttt ttctcagttg agtctgcccg ataaaaatcc aggacttcgt tgacgaggtt
     2161 cagaattttc tgcacgttgc gttctgcggc ttcaacaagt ggcttgagtg ggcccatgtt
     2221 gagatcttca tcatccttaa tcaattccag ggttgtgttg accacggtca gcggagactt
     2281 taaatcatga accagcgtcg cggtgaaatt ggctttaagc tgatccagtt ctttgagctt
     2341 caggtttgct tcttgcagat cacgggttcg tgcctcgact ttggcttcca gcatctggtt
     2401 tcggtgctca agcgcccggg tatagagtcg aaatgaaaga taaatcgtcg aagccaaaac
     2461 aaatgtatag actgcgtaag cccagattgt ttgccagggt gccgggcgaa tttgaatctg
     2521 aat
//
LOCUS       DAOQDO010000806         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36130_length_2523_cov_3.523501, whole genome shotgun sequence.
ACCESSION   DAOQDO010000806 DAOQDO010000000
VERSION     DAOQDO010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36130_length_2523_cov_3.523501"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2340
                     /locus_tag="PKZ53_13020"
     CDS             <1..2340
                     /locus_tag="PKZ53_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ41408.1"
                     /translation="ISLVRDITERKQAERALLESEERYREMFEADLTGNYISQPDGTL
                     ITCNQIFATMFGFETPEEAKQHSMASWYSTLRDHDAFLTDLCLNKRLFYPEVEVFRKD
                     GQSIFIAEIVAGVFDQSGTLTQLRGVCLDVTERKLASQALLDSQRQIALLVETIPYGI
                     LEIQPDGLITFANAYAHNIFDYTGGGEVVGHSAWLLLQPYGSPTLKAQFESFLDDQPI
                     PFTLEIETLTKFDRPIDLQVDWNYKRNQNDQVIGFVLVITDITERKRAKSALLKTEQR
                     LELVLQGANLGLWDWNLESDKVTYSQRWAEMLGYSIDEIEPTFQYQFHLTHLDDRPQV
                     EESIAAHLNNLIPFFQTEQRLQAKNGAWKWFLLSGKVIERSASGKPVRAAGTHLDITE
                     YKLLQGQLTQSQKMESIGRLAGGIAHDFNNLLTIILGYTQLLKRRFQHEEDATATSSL
                     LSIISSAERGATLTRQLLAFARQQTIEPHILNLNERITGMQSTILPLIGEHIEVKTIL
                     APALPKTKIDASQFEQILLNLIVNARDAMPTGGTLVIETQLVELGPEFNRQTTGLIPG
                     PYVMLAVSDNGVGIAPHNFEHIFEPFFTTKEQGKGTGLGLSTCYGIVKQNQGHITFYS
                     EPTVGTVFKVFFPVWQETNRRGTLPSLPLQIFRGTETILLVEDEPAVRNLARDILSDL
                     GYKVFEAGDGSLARDMAKNYPEPIHLLIADIVMPRLGGIELSHQIREFRPDMKVIYIS
                     GYTEGLVSVQKLLTEGTAFLPKPFSITQLLSRVRELLDE"
BASE COUNT          674 a          632 c          611 g          606 t
ORIGIN      
        1 atcagcctgg ttcgcgatat caccgaacgt aaacaggccg aacgggcact gttagagagt
       61 gaagaacgct atcgcgaaat gtttgaggcc gacctcactg gaaattatat ttcgcagcca
      121 gatggcacac tcattacctg caaccagatt tttgccacca tgtttgggtt tgaaacgccc
      181 gaggaagcca aacaacacag catggcgtcg tggtattcaa ccttgagaga ccatgatgcg
      241 ttcctgaccg atttatgttt gaacaagcga cttttttacc ctgaagttga ggtctttcga
      301 aaagacggtc agtcaatatt tattgctgaa atcgtggctg gggtgtttga ccaatctgga
      361 accctgaccc aattgcgagg ggtttgcctc gatgtgaccg agcgcaagct ggcgagtcag
      421 gcgctgttag acagtcagcg gcaaattgcc ctgctggttg aaacgatccc ttatggaatc
      481 ctggaaatcc aacccgatgg gctcattacc tttgccaatg cctatgcgca taacattttt
      541 gattataccg gcggcggcga agtggtcggt cacagtgcct ggctcctgct ccaaccctat
      601 ggttcaccca cactcaaagc tcagtttgaa tcatttctcg atgaccaacc gattccattc
      661 acgctggaaa ttgagacgtt aaccaaattt gaccgtccga ttgacctgca ggtggattgg
      721 aattacaaac gcaaccagaa tgatcaggtg attggatttg ttctggtcat tactgacatc
      781 accgagcgca aacgggccaa gagtgccttg ttaaaaaccg agcagcgcct cgaactggtg
      841 ttgcaggggg ccaatctggg gctatgggat tggaaccttg aaagcgataa agtgacctat
      901 agccagcgat gggccgaaat gcttggctat tcgattgatg agattgaacc tacctttcag
      961 taccagttcc atctgaccca cctggatgat cgcccacagg ttgaagagag catcgcagct
     1021 cacctcaaca acctcattcc atttttccaa accgaacaac gactgcaggc gaaaaatggc
     1081 gcgtggaaat ggtttctcct gagcggcaag gtcatcgaac gttcagcgag tggaaaacca
     1141 gttcgggccg ccggcaccca cctcgacatc actgaataca aactcttgca aggccaactg
     1201 acccaatccc agaaaatgga aagcatcggg cgactggctg gcgggattgc ccatgatttc
     1261 aacaacctgc tgacaatcat cctggggtac acccagttgc tcaaacgccg gtttcagcat
     1321 gaagaggacg caacggccac gtcatcattg ctcagcatca tcagttcggc tgaacgaggg
     1381 gccacgctga cccggcagtt actggctttt gcccggcaac aaaccatcga accacatatt
     1441 ctgaacctca atgagcgcat cacaggaatg cagagcacga tccttccgct cattggtgag
     1501 catatcgaag tcaaaaccat tttggcacca gccttgccga aaactaaaat tgatgccagc
     1561 cagtttgaac aaatcctgct caatctcatt gtcaacgccc gtgacgcgat gcccacaggt
     1621 ggtaccctgg tcatcgaaac ccaactggtt gaattgggtc ctgagtttaa ccggcaaacc
     1681 actggtctga tacctggccc ttatgtcatg ttagcggtga gtgataacgg agttgggatt
     1741 gctccacata actttgaaca tatttttgaa ccctttttca caaccaaaga gcagggaaaa
     1801 ggtacggggc ttggcctctc tacctgctat gggattgtca aacaaaatca agggcacatc
     1861 actttttaca gcgaaccaac cgttggaacc gtcttcaaag tgttttttcc agtctggcag
     1921 gaaaccaatc gccgggggac cctgcccagt ttgccacttc agatctttcg tgggacggaa
     1981 acgatcctcc tggttgaaga tgaacctgcg gtgagaaacc tggcgcgcga tattttgtca
     2041 gatttgggat acaaagtttt tgaagccgga gacggcagtc tggcccgaga tatggccaaa
     2101 aattatccag aaccaatcca tttgttgatt gccgatatcg tcatgccccg actgggcggg
     2161 atcgaattaa gtcaccagat cagggaattc cggcctgaca tgaaagtgat ttatatttcc
     2221 ggatataccg aaggtctggt ctcggttcaa aaactcctga cagaaggcac cgcgtttctg
     2281 ccgaagccat tttccattac tcaattgtta tcacgtgtcc gtgaactgct tgatgaataa
     2341 taccattttg gggtcatggt atcggggtca caggtcaggg aaatccaatt ctcccaatga
     2401 tttagccttt tgctaatacg agagcttcac tccaaaatgg tataaagagc attgagaata
     2461 aagaatgtgg tgagtggtta atgattagaa atcaatactt ttgaagaagc cccaagcccc
     2521 aag
//
LOCUS       DAOQDO010000807         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36223_length_2520_cov_2.432454, whole genome shotgun sequence.
ACCESSION   DAOQDO010000807 DAOQDO010000000
VERSION     DAOQDO010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36223_length_2520_cov_2.432454"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKZ53_13025"
     CDS             complement(<1..380)
                     /locus_tag="PKZ53_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ41409.1"
                     /translation="MSFTLLQPDELAPAVKLAEKVLSELDQILLGRTELHRLVLVGIF
                     SRGHILLEGLPGVGKTALVKALGQLLSLDFKRVQFTPDLMPGDILGSHILQDAADGTR
                     DMVFHQGPIFTNILLADEINRASP"
     gene            complement(602..2056)
                     /locus_tag="PKZ53_13030"
     CDS             complement(602..2056)
                     /locus_tag="PKZ53_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41410.1"
                     /translation="MSVHPLPKRSLFPATYEQELTSFRRQLARVLFLKYALLFLTMWA
                     FGYGILVLAIRVASQTPRTWLAWGLLGVLPVLVLAIIATRKQIPPIEKLRAVLDRESA
                     CGGLLMASHETKLEGWDGYVPHLTLPRVTWEGQRVLSLSGIGVVFLAASFLVPISSTV
                     MGMNQTLDVGKEVSQLNEQIETLKEEKLIDEPKADTLEEQLSKIQQEAKGDDPVKTLE
                     AIDHLDQNLKKTAQEAAEKDVQATERLSQQEALAETLSKEGAQMDDQLMAEATEELAE
                     MAKESLGENAPESQALKKQLSQDLKKALDSGKLTPEELKELAKALKNSKQSLADQLKK
                     LKESGLIDQKTLQQCEKAGQCNSQGLKDFLEKNKGDGQGEGSGKSVKESMKEWGRGGT
                     SRGEGNAPMSWTEGTTEEGAKFKEQTLPSNSLNPEDSQMTGLSVSEPKVENAGPQTGG
                     ALTGSTTGNASANTQKILPRHKGAVKRYFERKNK"
BASE COUNT          545 a          648 c          628 g          699 t
ORIGIN      
        1 ttgggcgatg cccggttgat ttcgtccgcc agcaggatat tggtaaaaat tgggccttga
       61 tgaaacacca tgtcccgcgt tccatctgcc gcatcctgca aaatatgtga tcccaaaatg
      121 tcaccaggca tcaaatctgg cgtgaattgc acccgtttaa agtcaagact gagcaattgc
      181 cccagcgctt tgaccagtgc cgttttccca acccccggaa gcccttcgag cagaatatgc
      241 ccccggctga aaataccgac caggaccagg cggtgcaact cggtccggcc cagcaagatt
      301 tgatcaagtt cagacagaac tttctccgcc agttttacgg ctggcgccaa ttcatcaggt
      361 tgtaagagtg taaaggacat aggttcaggg tgaaaaccag ggttcagggt tcagggttca
      421 gggttcaggg ttcagggttt agggttttag ggttcagggt tgacgaataa agaccaaaag
      481 gacatatggg acaaaaagga cgaaaattct aaaccctgaa tccgacatgg tttggctgtt
      541 tttaaaaacc ccacccgcta acgcaggtgg tactgactac tcttgatgca ggtggtactg
      601 actacttgtt tttgcgctca aaatagcgtt tgaccgcacc tttgtggcgg ggaaggatct
      661 tctgcgtatt ggctgaggca ttgcccgtgg ttgagcccgt gagtgcgcca ccggtttgcg
      721 gtccagcatt ttcaactttt ggttcactga cgctgagccc ggtcatctgg ctgtcttccg
      781 ggttgagcga atttgatggc agcgtttgtt ctttaaattt cgcgccttct tcagtggtgc
      841 cttcggtcca ggacattgga gcatttcctt cgcctcggga tgttccgccg cgtccccact
      901 ctttcattga ttccttgact gattttcccg acccttcgcc ctggccgtct cctttatttt
      961 tctctaaaaa gtccttgagt ccctgactgt tacactgacc tgctttttca cattgctgga
     1021 gggttttctg atcaatcaga cctgattcct tcagcttttt aagctggtca gccaggcttt
     1081 gcttgctgtt tttcaaggct ttggcgagtt ctttgagttc ttcaggagta agtttgcccg
     1141 agtcgagcgc ttttttcagg tcctgggaca gttgtttttt gagtgcctgt gattccggag
     1201 cattttcccc gagcgattct ttcgccattt cggccagttc ttcagtggct tccgccatca
     1261 gttgatcatc catttgagcg ccttctttcg aaagggtctc agccagcgct tcctgttgtg
     1321 acaggcgttc agtcgcctgg acgtcttttt cagcagcttc ctgagcggtt tttttcaggt
     1381 tctggtcaag atgatcaatg gcttcaaggg ttttgacggg gtcgtcacct ttagcttcct
     1441 gctggatttt ggagagttgt tcttcgaggg tatcagcttt gggttcgtca atgagctttt
     1501 cttctttcaa cgtctcaatt tgttcgttga gttgagagac ttcttttcca acatcaaggg
     1561 tctgattcat tcccatcacg gttgaagaga tcggcaccag aaaactcgct gccagaaaca
     1621 caaccccaat gccagacagc gagagtacgc gttggccttc ccaggtcacg cggggcagcg
     1681 tcaaatgtgg aacatatcca tcccagcctt caagtttggt ttcgtgcgag gccatcagca
     1741 atccgccaca ggcgctttcg cgatccagca ccgcccggag tttttcaatt ggggggattt
     1801 gttttcgagt cgcaataatt gccaggacca gcactggaag taccccaagc agtccccacg
     1861 ccaaccaggt gcgcggtgtc tgcgaagcca cccgaattgc cagaaccaga attccatacc
     1921 caaaagccca catggtcaaa aagagcaggg cgtatttcag aaagagaacc cgcgccagtt
     1981 ggcgccgaaa actggttaat tcctgttcat acgtcgccgg aaacagcgaa cgctttggca
     2041 aagggtggac agacatcaca gacgaccttt ctttggggct gaaaaaaaca gggctgaaga
     2101 cttcgggctg aagacttcgg gctgaagact tcgggctgaa gattcgcacg ctcagggctg
     2161 aaaacgtcga gctcagggct tggggctcag ggtattgaac ccgttttctt cagccccgag
     2221 cctgcgagtt ttcagcccca agccccaaac cctaaattgg gctgaagaat tggttttctt
     2281 tcatccctca tccttcatcc ctcatccctt tatgctgcgg tgagtcgcaa aaaatagcaa
     2341 ctcttttcaa ctcacgaaac tgactggtat tcgggtgagg ctgcggattg aacactcgtt
     2401 cgtttttcgg taacacaggt gtaagagtgt tgtaaccgtt cctcgtaaga tacagcttct
     2461 tgccttattt cactttcaac agttctctat tgacacatca gaactcaatt ggttccagct
//
LOCUS       DAOQDO010000808         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36266_length_2518_cov_2.760861, whole genome shotgun sequence.
ACCESSION   DAOQDO010000808 DAOQDO010000000
VERSION     DAOQDO010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36266_length_2518_cov_2.760861"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKZ53_13035"
     CDS             complement(<1..824)
                     /locus_tag="PKZ53_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ41411.1"
                     /translation="MGIQKKTRIIILGAGFGGLYTALNLEHSLARDPNVEITLVNREN
                     FIVFTPMLHEVAASDLDITHAVTPIRKLLRRVSFFNGDVEAIDLQNRKVVVSHGQEHH
                     HHDLEFDHLVIGLGSITNFYNLPGLEQHGMTMKTLGDAIHLRNHMISNLEEADFECCP
                     YVREPLLNFVIAGGGFAGVETIAGLNDFLRDAIKFYPHLREEMLRVILVHSGDVILPE
                     LGEQLGTYAQKKLAERKVEIHLKARVAEVTDREVILNNGTRIVTNTVVWTAGTSPNP"
     gene            complement(1141..1425)
                     /locus_tag="PKZ53_13040"
                     /pseudo
     CDS             complement(1141..1425)
                     /locus_tag="PKZ53_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1732..2238)
                     /locus_tag="PKZ53_13045"
     CDS             complement(1732..2238)
                     /locus_tag="PKZ53_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ41412.1"
                     /translation="MSENKLLHTVTTLYTRLVSIETLLQSPLLLIMRLYWGWQFFNTG
                     KGKLMHLDKTTEFFATLNLPAPYLNAMMAGSVECIGGLLLLLGLGSRLVTIPLTFTMI
                     VAYLTAHREELMGVFSNPDGFTEAAPFLFMLTAVIVLAFGPGIFSVDALIKKYVFNNT
                     NSESGSKA"
     gene            complement(2301..>2518)
                     /locus_tag="PKZ53_13050"
     CDS             complement(2301..>2518)
                     /locus_tag="PKZ53_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ41413.1"
                     /translation="ATLFLEGWIVGSWVEELTRECEQKLSQTERIILDVSQVSFVDDN
                     GVEVLKTLKQNRVHLTGCSLFLDELLK"
BASE COUNT          615 a          613 c          628 g          662 t
ORIGIN      
        1 gggttggggg atgtacccgc ggtccagacc acggtgttgg tcacgattcg ggtgccgttg
       61 ttgagtataa cttctcgatc cgttacctcg gcaaccctgg ctttgagatg aatctccact
      121 tttcgttcgg ccagcttttt ctgagcataa gtgccgagtt gttcgccgag ttccggcaaa
      181 atgacatctc cagagtgaac cagaatcacc cgcagcattt cttcgcgcag gtgtgggtaa
      241 aatttgatgg cgtcacgcaa aaagtcattg agtccagcaa tggtttcaac cccggcaaag
      301 ccaccgcccg caatcacaaa attcaacagt ggttcacgga cataggggca acattcaaaa
      361 tcagcttctt ctaaattgct gatcatgtgg ttgcgtaaat gaatcgcatc gcccagcgtc
      421 ttcatcgtca tgccgtgctg ttccaaacca ggaagattat aaaaattggt aattgagccc
      481 agtccaatca ccagatgatc aaattcaaga tcatggtggt ggtgttcctg accgtgagac
      541 accacgactt ttcgattctg taaatcaatt gcttccacat ccccattgaa gaaactcact
      601 cgccgtaata atttgcggat gggcgttacg gcatgagtaa tatcgagatc gctggcggcg
      661 acttcgtgca acatgggggt gaaaacaatg aagttctcgc ggttgacgag tgtgatttca
      721 acatttgggt cgcgggcgag cgaatgttcc aggttgagcg cggtgtagag tccgccaaaa
      781 ccagcaccta aaatgataat gcgtgttttt ttctgaatgc ccatcgcagt gttctcctga
      841 agtcaacaat gaagctacaa aagcccgagt tgtcaaaaag ggggtgaagc ttttgcagat
      901 ccattgtgag ataagctggt aggtcagggg gttgagacct gggtataccc aggtcctgat
      961 ccaaaacccc cgctgaaacc actcatctgg ttaattgccg cagccgtttt tgccaccaca
     1021 gctatttttt tctttagatg acttggattc attcttgctt ccgcagccat ctttcccacc
     1081 acagccattt ttgtccttgg atttatcttt tgaggtatct gctgctggtg ctgctgtttc
     1141 aggtggtttg ctgccgtcgg tggcacatcc accatggcct ttgcaggaat ttttactggc
     1201 acagccatta tccccggctt tgcagccgcc ctgtccttta cattcatttt tgcctttgca
     1261 cgaatgcttg gctgccgccg gagttgctgc gggttgtgac gttgcctgtt gcgcgggtgc
     1321 cgtggcgggt ttcgccgtat ctgaagatgg tgaagccgtg ctacatccgg ttaacacgcc
     1381 tgccacagcg gcgcctaaaa caaaggtaaa ttctctacga ttcatggttc ctccagggaa
     1441 aaattaaggg ttatccaccg ggtcaaaagt tgatcaccag cgggttgtga gcaatgtaac
     1501 tacttgacct gattcagttt tgctgcgctt gtgccccagc tttaccagcg tgcacaccaa
     1561 tcctgtttac ggattggggc acatccgtct caacggtgtc ggaatgctga gggggagcat
     1621 ttgtaaaaac ggggtgaaga aaggactggc caatgtagtg agcggtttat ccagtcatcc
     1681 cttcagaccc cagagcgact atggtttcga gcgtggttga atcaaaccgg gttacgcttt
     1741 tgagcctgat tcagagttgg tgttgttgaa cacatacttt ttaatcaggg catccaccga
     1801 aaatattccc ggtccaaaag ccagcacaat caccgctgtc aacataaaca ggaatggagc
     1861 cgcttcggta aacccatccg ggtttgaaaa cacacccatc agttcttcac ggtgggcagt
     1921 caggtaggca acaatcatcg taaacgtcaa gggaatggta accagtcgtg atcccaggcc
     1981 cagcaggagg agcagccctc caatacattc aaccgacccg gccatcatgg catttaaata
     2041 gggggcggga agattcaaag tggcaaagaa ctcggttgtt ttgtccagat gcatcaattt
     2101 tcctttgcct gtattgaaaa attgccagcc ccaatagaga cgcataatca gcaacagggg
     2161 gctttgcagc agggtttcaa ttgagaccag cctggtataa agcgttgtga cagtgtgtaa
     2221 taatttattt tctgacattg taagggatcc tctcgcggtt tcacggttgg ggaaaagagt
     2281 ggccaggcgt tgcgactggc ttatttgagg agttcatcca gaaacaacga acatccagtc
     2341 agatgaaccc ggttttgttt caaggtcttc aatacttcaa ctccattgtc atccacaaat
     2401 gaaacctgcg aaacatctaa aataattcgt tcggtttgtg acaatttttg ctcgcactcg
     2461 cgggttagtt cttcgaccca gctcccgaca atccatccct ccaggaaaag agtggcgg
//
LOCUS       DAOQDO010000809         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36285_length_2517_cov_3.003249, whole genome shotgun sequence.
ACCESSION   DAOQDO010000809 DAOQDO010000000
VERSION     DAOQDO010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36285_length_2517_cov_3.003249"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKZ53_13055"
     CDS             complement(<1..1202)
                     /locus_tag="PKZ53_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41414.1"
                     /translation="MLSQSKYPVRLFTFMTLLVGLACFWELALMTGSAAQSTPQARQL
                     TIEDRVRCQRAIEQVYWNHRVWPDENQLPKPSFSEVMPDSMLREQVETTLRQSNALEK
                     YWQRPITGEQLQAELTRIFTDSKDTTVLAEISTQLNHDPFLLAECLARPVLTERLIQS
                     WYARDVRIHGKQKAAIEAELKPGARLSELSGHYTETEWELSAESTQPGTSESFSTQSK
                     RKMSASEWKTETAKLTQLVGSKQPGSLPLGRLSGLQETDGSYFVVKVLAQTETSLKLA
                     RVEWPKHQFQDWWEQKQSEFSTMLTLPKNSYTISSNPVKGQFEPNAGLCESWTQINYG
                     FGSARINQTQVWTGTEMIVWGGHDLNGGIGTESFLPGVRYNPATDTWKPVRRSGSPAF
                     RLGSTAIWT"
     gene            1902..>2517
                     /locus_tag="PKZ53_13060"
     CDS             1902..>2517
                     /locus_tag="PKZ53_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ41415.1"
                     /translation="MPNFAPIAKSAEVLAYASVPKAISLSASDAEGSALQFELLTFPT
                     SGTLSGTVPNVQYTANSGFSGTDSFSFKVNDGQRDSARAFVTLYVIKDPFSANAGLDS
                     DRDGTQDVQVSHDETFLTIRSQGFPNHPTATFPNSKNPNSIRKQNHQFKIPLNPRKND
                     RVTGLPMGPIGVALNGIPFFNPFNAEGQVAVDGYSEEWLDACCGH"
BASE COUNT          586 a          644 c          607 g          680 t
ORIGIN      
        1 gtccatatcg ctgtgcttcc gagacggaaa gccggcgacc cgctccttct cacgggtttc
       61 caggtgtcag tggctgggtt atatcgcact ccgggcagga aactctccgt cccaatcccg
      121 ccgttgagat cgtgtccacc ccagacaatc atttcagtcc cggtccagac ttgtgtttga
      181 ttgattcggg cagatccaaa tccataattt atttgtgtcc aactctcgca caaaccggca
      241 tttggctcga attggccttt gaccgggttt gacgaaatgg tatagctgtt ttttggaagc
      301 gtgagcatcg ttgaaaattc agattgtttt tgctcccacc aatcttgaaa ttggtgtttg
      361 ggccattcca ctctggccaa cttgagggag gtttcagtct gtgccagaac tttcaccacg
      421 aaataacttc catcggtttc ttgtaatccg ctcaaacgcc cgagaggaag cgaccccggt
      481 tgttttgagc caaccagttg ggtaagtttg gcggtctcag ttttccattc tgaagctgac
      541 attttgcgtt tggattgagt ggaaaatgat tcggaagttc ctggttgggt agattcggct
      601 gacaattccc actccgtctc agtgtagtgc ccgctcaact cagacaatcg agcgcctggc
      661 ttgagttcgg cttcaatcgc cgctttttgt ttcccgtgaa tccgcacatc acgggcatac
      721 cagctttgaa tcagtcgctc tgtcaacact ggccgcgcca gacattccgc cagcaggaac
      781 gggtcatggt tgagttgtgt tgagatttca gccaacaccg tcgtgtcttt tgaatcggtg
      841 aagattcggg tgagttctgc ctggagctgc tcgccggtaa tggggcgctg ccagtatttt
      901 tccaaggcgt ttgactgacg aagtgtcgtc tcaacctgtt cccgaagcat cgaatccggc
      961 ataacctctg aaaatgatgg tttgggcaat tgattctcgt ctggccaaac ccgatgattc
     1021 caatagactt gttcaattgc gcgttgacag cgtacccggt cctcaatggt gagttgacgt
     1081 gcctggggtg ttgattgggc tgccgagccg gtcatcagtg ccagttccca aaaacacgcc
     1141 aatccaacca gcagggtcat gaaagtaaaa aggcgtacgg ggtactttga ttgagataac
     1201 attgctgttt ctcctgattt gatttctttt gtagcctgcc tgaagcagag ctgacaccgc
     1261 tccctaacct gaacaaatcc tgttccacat ttggtcagat ccagaaataa tcaataaagt
     1321 taagatgaac tcgttgagct ggttgtattt acatcaacag gtaatcgctg ggccgggatt
     1381 gggaaacgag tcaggtcatc tcaaaaagct atctcatcgg atagaggatc ttaccaaaaa
     1441 gatataccag gctctggctg actcgtttga cagtcggcct tatttttgaa cctctcctct
     1501 ctaccactgc gcagttgagg atggtgcttt ctcgcatttt ttctggtaaa aagtcaggtg
     1561 catcgctgat ccaaacataa tctttacaat tctcttcaac cgcccacata aaccctttac
     1621 attcgtttgg tagcctcggc tcaaattgac accaatgttg gttgcaggga ggccaaacca
     1681 gtatgaaaca gggttcaggg tttagggttc agagttcagg gttcagggct cggggttcaa
     1741 ggttcgggaa aaggcacaat cttcaagccg atcaaagatt caacttttta ttcacctacc
     1801 gaaatttcat tcacgtcatc agtctggttt gtttcacaac actttgtatt tccatcggat
     1861 ttccactccc gctcgcttct gccagttcat tcagggaaaa agtgccaaac ttcgcaccga
     1921 ttgcgaagag tgctgaagtt ctggcctatg ccagcgtgcc gaaagccatt tccctttcag
     1981 ccagcgacgc tgaagggagc gcattacagt ttgagttgct cacgtttccc accagtggaa
     2041 cactctccgg tacggtgcca aatgtccagt acacggccaa ttccggattt tccggaaccg
     2101 atagtttttc attcaaggtc aacgacgggc agcgtgacag cgctcgggcg tttgtcaccc
     2161 tgtatgtcat caaggatccg tttagcgcca atgctggcct cgatagcgac cgcgacggca
     2221 cccaggatgt gcaagtcagt catgacgaga catttctcac gattcgcagc cagggatttc
     2281 caaatcaccc aaccgcgaca tttcccaata gcaaaaaccc caattcgatt cgcaagcaaa
     2341 atcaccagtt caaaatccca ctcaatccca ggaaaaatga ccgggtcacc ggattaccga
     2401 tgggcccgat tggtgtggcg ctcaatggta ttccattctt caacccgttt aatgccgaag
     2461 gccaggttgc cgttgatggc tattctgaag aatggcttga tgcgtgctgt ggtcatc
//
LOCUS       DAOQDO010000810         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36305_length_2516_cov_3.340106, whole genome shotgun sequence.
ACCESSION   DAOQDO010000810 DAOQDO010000000
VERSION     DAOQDO010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36305_length_2516_cov_3.340106"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKZ53_13065"
     CDS             <1..256
                     /locus_tag="PKZ53_13065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ41416.1"
                     /translation="TVEAMSAGAVPVVINAGGQRESVCHARNGFLWNTLDEMLELTQS
                     LILDRELLERLGSQAMVDSNQFSREAFLHRIDRIIERLST"
     gene            295..1602
                     /locus_tag="PKZ53_13070"
     CDS             295..1602
                     /locus_tag="PKZ53_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNJ41417.1"
                     /translation="MRVLTWGLGYVGLVSSACLAHLGHEVIGIDSDTTKIQALNQGKC
                     LLKEPGLNEMINWNLTAGRLKGMKEDDVPANWADVLLICVGTPGAANGNYVLDDLKRV
                     VRTIGHRLRRIPSFQVVVVRSTLFPGTTRELVISLLEEYSGKKAGDDFGVVFNPEFLR
                     ESTAVKDFFDPPYILIGELDARSGRMVERLYESINTRKIRVGIEEAECLKLTSNAFHS
                     LKVGFANEIGRLCQQLNLDSQTVMDLVCADTRLNISSAYLSPGFAFGGSCLPKDLRSL
                     TTGARQMGITLPILEAVLPSNDLHLEMVLRKVRTHKFQSVGVLGLSFKSGTDDLRESP
                     AVELIDRLLQDGLNVRVFDPDVNLELLTGKNKAFLLERLAEIHSIWCTDINLIQSECD
                     GIVVSRAQPEFQAFIHQLKRTHPEILVIDLGAKNIGNSFGNEE"
     gene            1651..2109
                     /locus_tag="PKZ53_13075"
     CDS             1651..2109
                     /locus_tag="PKZ53_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41418.1"
                     /translation="MKTASWVVLALAGVLTLVFSLISVARAYGTYNDHIGGVSVSKLS
                     EGRPEVDTAMRARRATAAAYSAGFATLFLLITLVPYRRGEIWAWWALLVGMLVVSGLI
                     LLRIPLLDTGLAGPSGGGTAAASLIQLLLVGIGLALGAGRLRHKPADNPA"
     gene            2279..>2516
                     /locus_tag="PKZ53_13080"
     CDS             2279..>2516
                     /locus_tag="PKZ53_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HNJ41419.1"
                     /translation="MEELAILKKHILEGRYTDALAVVEDLEGMSRKAILMTIKSFLTR
                     LVSHLIKNDAEQRLTNSWSGSIRNSIVQIQGWNVM"
BASE COUNT          616 a          588 c          694 g          618 t
ORIGIN      
        1 aacggtggaa gccatgtcag caggtgccgt gccagttgtg attaacgcag gaggccaacg
       61 ggaaagtgtg tgccacgccc ggaatggttt tttatggaat accctggatg aaatgctgga
      121 actgacccag tcgctcatcc tggacagaga actgctcgaa cggcttggtt cgcaagcaat
      181 ggttgatagc aaccagttca gccgcgaagc ctttctccat cgaattgacc ggattattga
      241 gcgactttcg acttgaaact ggtcaacagg gactgaaaca ggggagaaaa acgaatgcgt
      301 gttttgacgt ggggattagg ctatgttggg ctggtttcat ccgcctgcct ggcgcatctg
      361 ggccacgaag tcattggaat tgattcagat accaccaaaa ttcaggcgct caaccaggga
      421 aaatgtttat tgaaagaacc tggattgaac gaaatgatca actggaacct cactgccggt
      481 cggttgaaag gaatgaagga agacgatgta ccggcaaact gggcagacgt tctgttgatt
      541 tgtgttggaa cacccggagc cgccaatggc aactatgtac tggatgacct caaacgtgta
      601 gtcaggacca ttgggcaccg gttgcgtcgg ataccttctt ttcaggtcgt tgttgtgcga
      661 agcactctgt ttcctggaac cacccgtgaa cttgtgatct cgctgcttga agaatattcg
      721 ggcaaaaaag cgggtgatga ctttggagtt gtgttcaatc ctgaatttct ccgtgaatca
      781 actgctgtca aggatttttt cgatccgcct tacattctga ttggtgaact tgatgctcgt
      841 tccggacgga tggtggaacg gctctatgaa tcaatcaaca cccggaaaat tcgggttgga
      901 attgaagaag ccgagtgcct gaaactcacc agcaatgcct ttcatagcct gaaagtagga
      961 tttgccaatg aaattggtcg cctgtgccag caactcaacc ttgacagcca gaccgtcatg
     1021 gatctggttt gtgccgacac ccggctcaat atttcatcag cctacctgag tccgggtttt
     1081 gcattcggcg gttcctgtct accgaaagat ttacggtcat tgacgaccgg agcacgtcag
     1141 atgggcatca ccctgccgat tctggaagcc gtgttgccca gtaatgacct tcaccttgag
     1201 atggtgctca ggaaagttcg gacccacaaa tttcagtcgg ttggtgtgct gggactcagt
     1261 ttcaagtcag gcaccgatga cctgcgggaa agcccagccg tcgaactgat tgaccggctt
     1321 ttgcaagacg ggctcaacgt cagagtgttt gacccggatg tcaatctgga acttctcacc
     1381 gggaagaaca aagcctttct cctggaacga ctggctgaaa ttcattcaat ctggtgtact
     1441 gacatcaacc tgattcaatc tgagtgtgat ggaatcgtgg tcagccgggc ccagcctgaa
     1501 tttcaggcat ttatccacca actcaaaaga acccatcctg aaattttggt gattgacctt
     1561 ggcgccaaaa acatcggcaa cagctttggc aatgaggaat aggtaccaaa aatagaatca
     1621 aaaacgtggt tgcagaaatg gagaagcaca atgaaaaccg caagctgggt ggtgctggct
     1681 ttggctggcg tgttgacact ggtgttcagt cttatttcag tggcaagagc atatggaacc
     1741 tacaacgacc acattggtgg ggtgagcgtg agcaaactat cggaaggacg acccgaagtg
     1801 gatacggcga tgcgggcgcg gcgggcaacg gcggcggcat acagcgctgg cttcgcgaca
     1861 cttttcctgc tgattaccct tgtcccctat cgtcgtggag aaatctgggc ctggtgggcg
     1921 ctactggttg gaatgctggt tgtttcgggg ctgatcctgc tcagaattcc actgctcgac
     1981 actggtttag ccggaccatc cggaggcgga acagcggcgg catccctgat tcaactgctc
     2041 ctggttggca taggtttagc gttgggagcc ggacgtttac gacacaaacc agccgacaac
     2101 ccggcgtgaa cagaggcaac gtgcgttcct tgtttggagt gtgctgacaa agtcagtgct
     2161 ttcatttttg gagtgcgctg acttgtcagc gctttcattt gcttgtgttg acgctggttg
     2221 gcggatggtc taattcgtgc gacaatccaa aacatcttgt ttccaaggag tttcgcgcat
     2281 ggaagaactg gcaatattga aaaaacacat tcttgagggc cggtacacgg atgcgcttgc
     2341 agtcgtggag gatctggagg gaatgagtcg aaaagccatt ttgatgacca ttaagtcgtt
     2401 tttgacccgt ctggtttcac atctcatcaa aaacgacgcc gaacagcgcc tcaccaattc
     2461 ctggtcaggc tcaatccgaa actcaattgt gcaaattcag gggtggaatg tgatgg
//
LOCUS       DAOQDO010000811         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36345_length_2515_cov_2.747561, whole genome shotgun sequence.
ACCESSION   DAOQDO010000811 DAOQDO010000000
VERSION     DAOQDO010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36345_length_2515_cov_2.747561"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /gene="gmd"
                     /locus_tag="PKZ53_13085"
     CDS             <1..1080
                     /gene="gmd"
                     /locus_tag="PKZ53_13085"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNJ41420.1"
                     /translation="AFITGITGQDGSYLAELLLAKGYTVHAMIRRASTFNTRWIDHIY
                     QDPHDPDARLFLHYSDLASTEWLADIMYQIRPNEMYHLAAQSHVRVSFDLPEYTADIT
                     GTSTVRILEAVRRSGVKTRFYQASSSEMFGNAPAPQSERTPFYPQSPYAAAKVYSYWM
                     TRMYREAYGLFACNGIFFNHESPRRGETFVTRKITRGLALILAGRQKKLYLGNLKAQR
                     DWGYAPEYVEAMWKILQQETPEDFVIGTGETHSVEEFVAEAFAYVGRDWREYVEIDPR
                     YYRPLEVDTLQADTQKARTQLDWTPSVGFKDLVKIMVDADLEANGIESPGEGKRILAE
                     KFTTWHHWDSAVTKLVQSTEGKASQ"
     gene            1094..2032
                     /locus_tag="PKZ53_13090"
     CDS             1094..2032
                     /locus_tag="PKZ53_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNJ41421.1"
                     /translation="MMKHSKRIAVTGGGGFLGSFLVERLKQAGYDNVVAPRKVEFDFF
                     DSAQVSRFYETVRPHIVIHLAAVVGGIGANRRHPGRLFYDNAIMGIQLIEQGRRFGLE
                     KFVCAGTVCAYPKFTPVPFREADIWNGYPEETNAPYGLAKKMLLVQLQAYRQEYGFNG
                     IYLIPVNLYGPRDNFDPQSSHVIPALIRKCVEAKRLNLPTITVWGTGAATREFLYVED
                     AAAAIVAAMEQYDRPAPMNLGSGQEMAIRELVMLIRELVGYTGDVVWDASQPDGQPRR
                     CLDTSLATQELGWTAQTPFREGLQRTIDWYLAHSTQ"
     gene            complement(2072..>2515)
                     /locus_tag="PKZ53_13095"
     CDS             complement(2072..>2515)
                     /locus_tag="PKZ53_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ41422.1"
                     /translation="GLVLCCSFLNCPISKQVHPLLKLIGRVVFQLPVPVIAVHHWMIG
                     WEADWKLAKEICQVLESVPGEVVFQRVNAVLDVEVSQIFQNLCQPILVINAEQDQLIP
                     SLNIGLPSKSSLITVRNIPGPHLLLQTHPAQAWEQIHSFLKQHHL"
BASE COUNT          641 a          619 c          663 g          592 t
ORIGIN      
        1 gcttttatca ccggcattac cgggcaggat gggtcatatc tggcagaact gcttctggcc
       61 aaaggttata cagtgcatgc gatgattcgg cgggcttcga cattcaatac ccgctggatt
      121 gaccatattt atcaggatcc gcacgacccc gacgcccgtc tgtttctgca ttattctgat
      181 ctggcaagca cggagtggct ggctgatatc atgtatcaaa tccggcccaa cgaaatgtac
      241 caccttgccg cacaaagcca cgtcagggtg agttttgact tgcctgaata caccgccgac
      301 attactggaa ccagcacggt gcggattctt gaagccgtcc gccggagcgg tgtcaaaacg
      361 cgattttatc aggcatccag ttccgaaatg tttggcaacg cgccagcgcc tcagagtgaa
      421 cgaacgccgt tttacccaca gagcccctat gcggcggcca aagtctattc gtactggatg
      481 acccgcatgt accgcgaagc ctatggcctt tttgcctgca atgggatttt tttcaaccat
      541 gaaagcccac ggcgcggcga aaccttcgtg actcgaaaaa ttacccgcgg actggcattg
      601 atcctggctg gccgtcaaaa aaagctttat ttgggaaatt taaaggccca gcgggattgg
      661 ggatatgctc ccgaatatgt cgaagccatg tggaaaattc tccagcaaga aactcctgaa
      721 gatttcgtca tcggaaccgg tgaaacgcat agtgttgaag aatttgtcgc cgaagcgttt
      781 gcctatgtgg gccgcgattg gcgcgagtat gtcgaaattg accctcgata ctaccgcccg
      841 ctggaagttg atacgttgca ggcagatact caaaaagcca gaacacaact cgattggaca
      901 cccagcgttg gttttaagga tttggtaaag atcatggttg acgctgacct tgaagcaaac
      961 gggattgagt ctcccggtga aggcaaacgc atcctggccg aaaaattcac gacctggcat
     1021 cactgggatt cagcggtgac aaagcttgta caatcaacag aaggaaaggc gagtcaataa
     1081 accataagga taaatgatga agcactcaaa acgcattgct gttaccggtg gaggtggttt
     1141 tctcggctcg tttttggttg aacgattaaa acaggcgggg tatgacaacg tagttgctcc
     1201 gcgcaaagtc gagtttgact tttttgactc cgcacaggtc agccggtttt atgaaacagt
     1261 acgcccacac attgtcattc atctggcggc ggtggttggc ggtatcggtg ctaaccgccg
     1321 gcatccaggg cgattatttt acgacaacgc catcatgggg attcagttga ttgaacaggg
     1381 gcgacgcttt gggcttgaga aatttgtttg tgccggaacg gtgtgcgcct acccgaaatt
     1441 cacgcccgtg ccgtttcgag aggccgatat ttggaatgga tacccggaag aaaccaacgc
     1501 cccgtatggg ctggccaaga aaatgcttct ggtgcagttg caagcctatc ggcaagaata
     1561 cggttttaat ggcatctact tgattccagt gaatttatat ggtccacggg ataatttcga
     1621 cccgcaaagc tcacatgtca ttccggcgtt gattcgcaaa tgtgtcgaag ccaaacgact
     1681 gaacctccca accatcactg tctggggaac gggggcggcc actcgcgaat ttttatatgt
     1741 tgaagatgcc gccgccgcta ttgtggcagc gatggagcaa tatgatcgcc cggcgccgat
     1801 gaatcttggg tcaggccagg agatggcaat tcgggaactg gtgatgttga ttcgggaact
     1861 ggttgggtat accggagacg tggtgtggga tgcgtcgcaa cctgacggcc agccacgccg
     1921 ctgtctcgat accagtttgg caactcagga actagggtgg accgcacaaa ccccttttcg
     1981 ggaaggttta caacgaacga ttgactggta tctggctcac tcaacccagt aaaagcaaag
     2041 cgatgtcaag ccatcacact cgacagcact cttacaggtg gtgttgtttc aaaaaggaat
     2101 gaatctgttc ccaggcctga gctggatgag tttgaaggag taaatgcggg ccgggaatgt
     2161 ttcttactgt tatcagactt gatttggacg gcaacccgat gttaaggctt ggaatcaatt
     2221 gatcttgctc ggcattgatg accaggatcg gctgacaaag gttttgaaaa atttgactga
     2281 cttcgacatc aagcaccgca ttgactcgct gaaaaacgac ctcacccggc actgactcca
     2341 gcacctgaca aatttctttg gccagcttcc agtcggcttc ccagccaatc atccagtgat
     2401 gaacggcaat cactggaact ggaagctgaa acaccacccg tccaatcagt ttcaggagtg
     2461 gatggacctg cttactgatt ggacagttca aaaatgaaca acacaacacc agtcc
//
LOCUS       DAOQDO010000812         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36497_length_2509_cov_2.632029, whole genome shotgun sequence.
ACCESSION   DAOQDO010000812 DAOQDO010000000
VERSION     DAOQDO010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36497_length_2509_cov_2.632029"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKZ53_13100"
     CDS             <1..516
                     /locus_tag="PKZ53_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41423.1"
                     /translation="KFIRILFFSVVWVVIGTGLGMAQEVPKAEVFAGYSFTRLNGTAP
                     GEGVNANGASVSVAATVHKAVSLVADFGGYAGEGTTMYSYTFGPRFAYHSDAKITPFA
                     HFLFGGAKLSNPGNSSGFAFIGGGGLDYKMTDRVSVRVIQGELFHTNLLDSRQSHLRL
                     STGIVFRFGKR"
     gene            624..>2509
                     /locus_tag="PKZ53_13105"
     CDS             624..>2509
                     /locus_tag="PKZ53_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ41424.1"
                     /translation="MQFFRNLTGNHLSGKGWPVLSLALHCGWILAALLFFSTAIQAQS
                     TNGRLVITAKDQSGAVVAGAEVQLLNLGTGQQLTSTTNEDGFVIFPQLPVGFYTVSIE
                     VPGFKKSVTENVKVDVGQEYGLSVSLEAGGATDVVTVAAGEELVQTTNAEVKNTVNQK
                     QVQDLPLNGRDPLQLIQLQAGVAGGASSARANVTTTINGQRASTATVTQDGINIQDNL
                     FRENSLDLAVNRPTVAQVAEFSVISQNAGAEVSGGASAIRTVTPSGTNEYHGSLYEFH
                     RNSALGSNDFFNNLQGVAKPQLIRNQFGGTVSGPIKKDKIFIFGVYEGYRERTASPTT
                     TTTFLPDARNGIFTYRDNQGQIRKINLLGLRNLSVDPFSQGLLGQIPTNANATTAGDG
                     LNTSGYSFNQSSDWDRNTMTVRTDVLLNSHHRLEGVYNRASEQVERPDIDTTFALNPG
                     INNTNVTHFGVGAWIWTITNNLNNEFRVGGNRNEPLFNSNADTSSFQVVIPAIVTNPV
                     NLFTNQGRSTSTMSFIDSASWAKGEHFVRFGGQYDRIRTNPFFFSAGSPPAFTLGFGV
                     KTPAEIPLVGRDFPGGIDATQLNTANNLLAFLAGVITAGTVTYEVTSKDSGYTQGAVN
                     QRHYL"
BASE COUNT          589 a          651 c          643 g          626 t
ORIGIN      
        1 aagtttatac ggatcctttt tttctccgtt gtatgggtag tgattggaac tggattggga
       61 atggcccagg aagttccgaa agccgaagtg tttgccgggt attcatttac ccgactcaat
      121 ggcacggctc ccggagaagg cgtcaacgcc aatggggcca gtgtttcagt ggctgccaca
      181 gtgcataaag cggtatcgct ggtggccgat tttggcggct atgccggtga agggaccacg
      241 atgtattcct atacattcgg gccacggttt gcctatcact ctgacgcaaa aataacaccc
      301 tttgcgcatt ttctgtttgg cggtgccaag ttgtcaaacc ctggaaacag ctcaggtttt
      361 gcctttattg gcggcggcgg gctggattac aaaatgacgg atcgggtttc agttcgggta
      421 attcaaggtg aattgtttca tacaaatctg cttgatagcc ggcaatcaca cctgcgtctt
      481 tcaaccggga ttgttttccg atttggtaaa cgataaccgc tcactttgga gtgctgtgac
      541 ttgtcacagc tttggtctcc agcggcttga cgctgaaaag tgaattcacg ctgcgggctg
      601 gttttttcac cgagggaggt tttatgcagt ttttcagaaa tttgacaggg aatcacctgt
      661 cagggaaagg atggcctgta ctttctctgg cgttacattg cggctggatt ctggccgcac
      721 ttttattttt ttcaacagcg atacaggctc aaagtaccaa cgggcggcta gtcataaccg
      781 ccaaggatca atccggagcg gttgtggccg gtgccgaagt ccagttgctc aatcttggca
      841 ccggtcaaca actgacatcc accaccaacg aagatggatt tgtcattttt cctcagcttc
      901 cggttggatt ttacaccgtt tcaatcgaag ttccgggctt taagaaatca gtaactgaaa
      961 acgtcaaagt tgacgtcggt caggaatatg gactttcggt ttcgctcgaa gccggtggtg
     1021 ccactgatgt ggtaaccgtg gccgccggtg aagaactggt ccagaccacc aatgccgaag
     1081 tcaaaaatac ggtgaatcag aagcaagttc aggatttgcc gctcaatggt cgtgacccgt
     1141 tacaactcat ccaattgcaa gccggtgtgg ctggtggagc cagcagcgcc cgtgccaatg
     1201 tgacgaccac gatcaatggc cagcgggctt cgacggcaac cgtgacccag gacggcatca
     1261 acatccagga caacctgttc cgagaaaact cgcttgatct ggcggtcaat cgtccaaccg
     1321 tggcgcaggt cgctgaattc agtgtgattt cacagaatgc cggtgccgaa gtttccggcg
     1381 gcgcctcagc cattcgaact gtgacgccat cgggcaccaa cgaatatcac ggttcactct
     1441 atgaatttca ccgcaacagc gcgcttggct ccaatgactt tttcaacaat ctgcagggcg
     1501 tggccaaacc gcagttgatc cgcaaccagt ttggcggcac agtttccggt ccaatcaaaa
     1561 aggataaaat ctttattttc ggtgtgtatg aaggctatcg tgaacgaacc gccagcccga
     1621 ccaccacgac gacctttttg cctgatgccc ggaatggcat ttttacgtat cgcgacaatc
     1681 aggggcagat tcgaaaaatc aatctgcttg gtttgcgaaa cctgtcagtt gatccgttta
     1741 gccagggatt gctcggtcag attccgacca acgccaatgc cacaaccgct ggagatggct
     1801 taaatacatc aggatattcg tttaatcagt cttcagactg ggatcgcaac acgatgacgg
     1861 ttcgcaccga cgtcctgctc aattcccatc atcgcctgga aggcgtgtat aaccgcgcca
     1921 gtgaacaggt cgagcgacct gacattgaca cgacgtttgc gctcaatcca gggatcaaca
     1981 acaccaacgt tactcacttt ggtgtcggcg cctggatttg gacgattacc aacaacctca
     2041 acaatgaatt tcgcgtaggc ggaaaccgaa acgaaccgct ctttaattca aatgctgaca
     2101 ccagttcgtt tcaggtcgtc attccggcga ttgtgaccaa cccggtcaat ttgtttacca
     2161 accagggccg atcaacctca accatgtcct ttattgactc ggcgtcgtgg gccaaaggcg
     2221 aacattttgt ccgctttggt ggccagtatg accggattcg gaccaatccg tttttcttta
     2281 gtgcgggctc gccgccagcg tttacgcttg ggtttggtgt gaaaacaccg gctgaaattc
     2341 cactcgtggg ccgtgacttc cccggcggca ttgatgcgac ccagttaaat accgccaaca
     2401 acctgcttgc ctttttggcc ggcgtgatta cggctggaac cgttacctat gaagtcactt
     2461 caaaagactc agggtacacc cagggggcag tcaatcaacg ccattacct
//
LOCUS       DAOQDO010000813         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36534_length_2507_cov_3.080343, whole genome shotgun sequence.
ACCESSION   DAOQDO010000813 DAOQDO010000000
VERSION     DAOQDO010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36534_length_2507_cov_3.080343"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..>2507)
                     /locus_tag="PKZ53_13110"
     CDS             complement(121..>2507)
                     /locus_tag="PKZ53_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41425.1"
                     /translation="HTFEANTRNSLGRVYLVQGKFQPALDELKLALKLATQTTNLGLQ
                     SMILTTIGNLYRLTGEQAKALATLNQALALSKESGDRRQTAGALLILARLHQTEGNWP
                     LTRSVCLQALEIVKQLGLKRLEAEGYQIIAAVEDLEGNTLTAQEQYQKALEIRQAIED
                     RIGTGNTLINLAGIQTRLGNTQKALDYQKQALEVFTQIEDPQGQVRAFMVLAQTHARI
                     GDTQTAIANLEQAIHRIETLRSSIADIETRASFFTTVQRTFKLYISLLMQPGNSPDAI
                     ARAFEANETSRARGLLDLLTAAQAKINVGIEPNLIAQEEFIRQKMIDQSSYLSRLFNQ
                     PRPDTQKIELVRTEIQRLSEAFTEVEAQIRDKYTRYAELTQPTPLSVKVLQQQILDPE
                     TTLVEYCLGEEESYAWVVTQTEIKAFRLPKQSDIEAQARAFCQALSFQPGPTRDLKLA
                     NLTPLKTSLKQAGKNLSQAILRPLLPALKTRRVLLVADGALNFVPFSALPIEHPGQPG
                     QQRPGKLRMLEQYEIVNLPSASILTRLLSEPQPQSVIQKRLTIWADPVFSALDPRLSQ
                     TKHKTQASNPARPRNQSTPINKLDETPLWNELNEIEIPRLPGTQTEVQFLKQLIPASN
                     LFVASGFQANRQTLMETDLSQFQIVHFATHGYVSSEHPENSGLIFALRNEQGEKINGF
                     LPLADVYQLKLNADLVVLSACETGLGKVLLGEGLVGMTRGFMYAGTRRVVVSLWAVSD
                     RGTAELMKRFYRRMLTRQESPAAALRGAQLELLRQTKYKSPFYWAPFVIQGDFR"
BASE COUNT          560 a          610 c          666 g          671 t
ORIGIN      
        1 attgctcggg cttcgggttc agccattccc aaatcaaaag cacttgaaat catcgttgag
       61 gctctggagc agcaacacct gacactgtgg agtgcggcgg cttgacgccg ctttatgccc
      121 ttaccgaaaa tcgccctgaa tcacaaacgg cgcccagtaa aaaggtgact tatatttcgt
      181 ttgccgcaaa agttcgagct gggcgccccg gagtgcagcc gctggagatt cctgccgggt
      241 cagcattcgt cggtaaaagc gcttcatcaa ctctgcggtt ccccggtcgc tgactgccca
      301 caaactgacg accacccggc gggtgccagc atacataaac ccacgagtca tccccaccag
      361 accctctcca agtaaaacct tgcccagccc agtttcacaa gccgaaagca cgaccagatc
      421 ggcattgagt tttaactggt agacatctgc aagcggcaag aacccattga tcttttcacc
      481 ctgttcgttc cgaagtgcaa aaattaagcc tgaattctca ggatgctctg agctgacata
      541 cccgtgagtc gcaaaatgca caatttggaa ctggctcaaa tcagtttcca tcaaggtctg
      601 ccgattggcc tgaaaaccgc tggcaacgaa caggttggaa gccgggatta gctgtttcaa
      661 aaattgcact tcggtttggg tccctggtaa tcgcggaatt tcaatttcgt tcaattcgtt
      721 ccagagaggg gtttcatcca gtttatttat tggggtgctt tggttgcgcg gacgggctgg
      781 attggaagcc tgggtcttgt gctttgtctg gcttaaacgg gggtcaagag ctgaaaagac
      841 cggatcagcc caaatcgtca gccgtttttg gatgacagac tgtggttgtg gttcagaaag
      901 gagcctggtc aggattgaag ccgatggtaa attgacaatt tcatactgtt ccagcattcg
      961 aagttttcct ggtctttgct gaccgggctg tccggggtgc tcaatgggta aggcactaaa
     1021 cgggacaaaa tttaatgccc catctgcaac cagcaaaacc cgtcgggttt ttaaagccgg
     1081 gagaaggggc cgcagaattg cctgactgag gtttttcccg gcttgtttca aggaagtttt
     1141 gagcggggtg aggttggcaa gttttaaatc gcgggttggc ccaggttgaa aagacagcgc
     1201 ctgacaaaag gctctggcct gggcttcgat atcgctctgt ttcggaagcc gaaacgcttt
     1261 tatttcagtt tgggtcacaa cccaggcata actttcttct tcgcccagac agtattcgac
     1321 gagggtggtc tcaggatcta aaatctgttg ttgcaatact tttactgaaa gtggcgttgg
     1381 ctgggtcagt tcggcatagc gggtgtattt gtcccggatt tgtgcctcaa cctcggtgaa
     1441 agcttcgctc aatcgctgga tttctgttcg tacgagttcg attttttgag tgtcaggtct
     1501 gggctggttg aacaaccttg acaggtaact cgattgatca atcatttttt gccggataaa
     1561 ttcttcctgg gcaatcaggt tgggttcgat tccgacattg attttggcct gggccgccgt
     1621 taataaatca agcagtccgc gtgcccgaga cgtttcgttg gcttcaaagg ctctggcaat
     1681 ggcgtcgggg ctgtttccgg gctgcatcaa gagggaaata tataacttga acgttctttg
     1741 aaccgtcgta aaaaaactcg ctcgggtctc gatatccgca atactggacc gaagggtttc
     1801 aatgcgatgg attgcttgtt cgagattggc aatggcggtt tgggtgtcgc cgattcgggc
     1861 atgagtctgg gccagaacca taaatgcccg cacctgaccc tggggatctt caatctgggt
     1921 aaacacctca agtgcctgtt tctgatagtc aagtgccttt tgggtatttc ccagcctggt
     1981 ttgaatccca gccagattga tcaaggtgtt tccagtaccg attcgatctt caattgcctg
     2041 gcgaatctcc agtgcctttt gatattgctc ctgcgcagta agcgtatttc cttctaaatc
     2101 ctcaaccgca gcaataattt ggtacccttc agcttctaac cgcttcagcc cgagttgttt
     2161 gacaatttca agtgcttgca ggcacaccga gcgggtgagt ggccagtttc cctctgtttg
     2221 atgaagccgg gcgagtatca gcaacgcccc ggctgtttgg cgacggtcac cggattcttt
     2281 tgagagggcc agggcctgat tgagtgtggc cagggctttg gcctgttcgc cagtcaaccg
     2341 gtacaaattc ccaatcgtgg tcaggatcat ggactgcaat cctaaattag tggtctgggt
     2401 cgccagtttc agtgccagtt ttaactcatc caatgcaggt tggaattttc cctgaaccag
     2461 gtacacgcgg cccaggctat tgcgggtatt ggcttcaaag gtatgat
//
LOCUS       DAOQDO010000814         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36578_length_2505_cov_3.885306, whole genome shotgun sequence.
ACCESSION   DAOQDO010000814 DAOQDO010000000
VERSION     DAOQDO010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36578_length_2505_cov_3.885306"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKZ53_13115"
     CDS             complement(<1..194)
                     /locus_tag="PKZ53_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41426.1"
                     /translation="MSAVCVTTQLQFKSVSAEKQFTEPGAAQVMRAIPATDAEEPSLL
                     PAIQDELIFDTNRLTVDANAT"
     gene            complement(396..2141)
                     /locus_tag="PKZ53_13120"
     CDS             complement(396..2141)
                     /locus_tag="PKZ53_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNJ41427.1"
                     /translation="MSVQAGESAMFQANLNHTGVYSTKAAAQFTGIKWKFVTNGPVRS
                     SPTLFDGVVYFGSSDQFCYAVQSQTGRQVWKFQAGGAVHSAPACTTTDVYFTDSKGTL
                     YDLGRADGKLKWKLETANHPYIGGWDFLISSPIVTNTLVYFGSGDGYLYAVEKATGKQ
                     KWRFQTGEVIRSTPAVADGLLYIGGFDGKLSALDAETGQARWEFKTIGNQFFPKGEIQ
                     GSPAIVDGIVCFGSRDYHLYALDAKTGKELWKVMHENSWVITTPVIHNGVVYAGSSDG
                     KFVQAVDLKTGVEKWHVATTSNVLASPALAEDVVYFSQMDGVFLALDATTGKEKWKFT
                     ATDPIFSSPVVADSVVYVGCDDGGLYALSGEPADTFQPAVWKAVYWDEKVVYHWFEHH
                     VPVRDHFVKAGYTQIDSVGLYRFMNRRIKDKTPSVIVFAMDCVPGFLASEISEKALLR
                     QYLNAGGKIVWLGEPPLAVIKDENTGLPYNFNPERTTKVLGFNDKDADSGLYAMKATP
                     EGKKWGLPDSWVGSWPIDPKEVTTVLGTDEFGRPMAWVKNYGGPEGTGFVRLWGRQRA
                     IDNLPAIQAIAEYGL"
     gene            complement(2319..>2505)
                     /locus_tag="PKZ53_13125"
     CDS             complement(2319..>2505)
                     /locus_tag="PKZ53_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41428.1"
                     /translation="GGGEIVFPEPVPEVVIIKRFQRIAPKFTGPLAGQVVDISNYSRK
                     EIDLPGVCTMKMLARQF"
BASE COUNT          620 a          702 c          587 g          596 t
ORIGIN      
        1 gtggcattgg cgtccacggt caatcggttt gtatcaaaga tcagttcatc ctggattgcc
       61 ggcaaaagtg acggctcttc tgcatctgtt gcaggtatcg cccgcatgac ctgagcagcg
      121 cctggttcgg tgaactgttt ttcggcactc accgatttga attgaagctg tgtggtgacg
      181 catacagcac tcacaatgaa aaaaccaaca ctcgccaacc tgtttcttcg tgacttccgc
      241 atagcaacct ccatcgtgac ccgacaagct ttttgacgag gttaaagcta tgcgaaaatt
      301 gagcttattc aattcttttg cgacggatga cacgcagacg cgacgaagag cacacccacg
      361 gtgaagagtt ccgccttttc ggcggaacgc ttgacctaaa gtccatactc cgcaatcgcc
      421 tgaatcgccg gcaagttatc aattgccctc tggcggcccc acagtcggac aaagcccgtg
      481 ccttcgggtc cgccgtagtt cttgacccag gccattggac ggccaaattc atcggtgccg
      541 agaaccgtag tgacttcctt cggatcaatc ggccaggaac caacccacga atcaggcagg
      601 ccccattttt tcccttcggg tgtggccttc atagcgtaaa gcccggaatc ggcatccttg
      661 tcgttgaatc caagcacttt cgtcgttcgc tcagggttga aattataggg caacccggtg
      721 ttttcatctt tgatcactgc cagcggaggc tctccgagcc agacaatttt cccgccagca
      781 ttcagatatt gacgcagcaa cgctttctct gaaatttccg atgccagaaa acctggaaca
      841 caatccatcg caaagacaat cacgctcggg gttttgtctt tgattcgccg attcataaac
      901 cgatacagtc cgacggaatc aatttgcgtg tacccggcct tcacaaaatg gtcgcggacc
      961 gggacgtgat gttcaaacca gtgatagacg actttttcat cccagtacac ggctttccac
     1021 acggctggct ggaaggtgtc tgctggttca ccggacagag cgtagagtcc tccgtcatca
     1081 cacccgacat agaccacact gtcggcgacg actggcgaag aaaaaattgg gtcagtcgcc
     1141 gtaaacttcc atttctcttt ccccgtggtc gcgtccagcg ccagaaaaac accgtccatc
     1201 tgggaaaaat acaccacatc ttcagccagt gccggcgaag ccagcacgtt tgaagttgtt
     1261 gccacatgcc atttctccac gccggttttg aggtcaaccg cctggacaaa tttcccgtct
     1321 gaactgccgg catagacaac tccattgtga atgaccggag ttgtaatcac ccacgaattc
     1381 tcgtgcatta ccttccacag ctcttttccg gttttggcat caagcgcata taaatgatag
     1441 tcacgactgc caaaacacac aattccgtct acaatcgcgg gtgaaccctg gatctcacct
     1501 tttgggaaaa actggttccc gatggtttta aattcccagc gagcctgccc ggtttcggcg
     1561 tccagtgccg aaagtttccc gtcaaatccg ccaatgtaga gcagcccgtc cgccaccgct
     1621 ggtgtcgaac gaatcacttc gcccgtctga aaccgccatt tttgtttccc cgtggctttt
     1681 tcaactgcat acagataccc atctccgctg ccgaaataga ccagcgtatt tgtaacaatc
     1741 ggcgacgaga tgagaaagtc ccagccaccg atatatgggt gatttgccgt ttccagcttc
     1801 catttcagct tgccgtccgc tcgacccaga tcatagagcg tgccttttga atcggtgaag
     1861 tacacatcgg ttgtggtaca ggctggggcc gaatgaaccg cgccaccagc ctgaaacttc
     1921 cagacctgac gtccggtttg ggattggacg gcataacaaa actgatcgct gctgccgaaa
     1981 tacacaacgc cgtcaaacag cgtcggcgag gaccgcaccg gaccatttgt aacaaacttc
     2041 catttgatgc ccgtaaactg ggcggcagct ttcgttgagt aaacgcctgt gtggttgagg
     2101 tttgcctgaa acatggcgga ttcgccagcc tgaacagaca aaaaactcac cagcaggcac
     2161 aatatggcca agccaggtga atatcgaagc atagtgtggg attgattttt ggtgaaaact
     2221 gagaggtggg cctggaattg tcgcccaaaa cggcaaaaag cgaactaaag ttcaccaaat
     2281 cacttcaaaa cctgattttt tgaagttcga gctattcatc aaaactgccg cgcaagcatc
     2341 ttcatggtac acacacctgg cagatcaatt tcttttcgtg agtagttcga aatatcaacc
     2401 acctgtccgg ccagcggacc agtgaatttt ggcgcaatgc gttgaaaacg tttgataatc
     2461 acaacttcag gaactggctc tggaaatacg atttcgccac ctccc
//
LOCUS       DAOQDO010000815         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36580_length_2505_cov_3.412245, whole genome shotgun sequence.
ACCESSION   DAOQDO010000815 DAOQDO010000000
VERSION     DAOQDO010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36580_length_2505_cov_3.412245"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2505
                     /locus_tag="PKZ53_13130"
     CDS             <1..>2505
                     /locus_tag="PKZ53_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNJ41429.1"
                     /translation="ARTGINRYQIAPHTVFVSHETYTPARGGSAAAEVHALRQVFGDK
                     ASQLVIANTKGFTGHPMGVGIEDVVAIKAVETGIVPPVANFKEVDPDLGVLNLSKGGA
                     YPIHYALRLGAGFGSQISMTLIRWIPTADGMHRAPEALGYEYRVYDRTQLNQWLSTIS
                     GYPTTQLEVINRTLRIKDQGAPALPLTTAVPKPVVAAPVTSWAQPAAVPTPVAIPPAP
                     AKVEPATVVAKVEAPPAAPAFTSVPTPVPVFDAVKEKVLSLAAQKTGYPVDMLDLDLD
                     LEADLGVDTVKQAEMFAAIRGAYDIPREENLKLRDFPTLKHVIKFVYDRRPDLKAVEL
                     PPLPTKVVAMASGVGAAPALAPVIAVATIDPVREEVLKLTAQKTGYPIDMLDMDLDLE
                     ADLGVDTVKQAEMFAAIRGAYTIPRDENLKLRDFPTLKHVVKFVYDRRPDLKAPEPAV
                     AAVAVTAQAPVATPAAPAVDEVTQKVMALAAEKTGYPPEMLDLDLDLEADLGVDTVKQ
                     AEMFASVRAMFDIPRDENLKLRDFPTLKHVIGFVYAKRPDLKVVAAPAPVATAPVQVT
                     APAAAPAAPVMDEVTEKVLALAADKTGYPSEMLDLDLDLEADLGVDTVKQAEMFASVR
                     AMFDIPRDENLKLRDFPTLKHVIGFVYDKRPDLKVAAAPAPVQPVAAPVAEAAPAVDE
                     VTQKVLDLAVEKTGYPAEMLDLNLDLEADLGVDTVKQAEMFASVRGLFDIPRDENLKL
                     RDFPTLNHVIGFVYDKRPDLKGAAATQATAATAGAAASAVAAPAVPQTMSLDAAAAIP
                     RRVPVPILRPALADCKPTGVKLEAGTRVVVMPELGA"
BASE COUNT          570 a          755 c          678 g          502 t
ORIGIN      
        1 aagcccgcac cggtatcaat cggtaccaaa tcgcccctca taccgtgttt gtctcgcacg
       61 agacctatac tccggcccgt ggcggaagcg ccgcggctga agttcacgcc ctgcgccagg
      121 tctttggcga caaagccagc cagcttgtga ttgccaatac caaaggcttt accggccacc
      181 cgatgggtgt tggaattgaa gatgtggtgg cgatcaaagc ggttgaaacc ggcatcgtcc
      241 cgccagtggc gaatttcaag gaagttgacc cggatttggg tgtgctcaat ctgtcaaaag
      301 gtggcgccta tccgattcac tatgccctcc gactgggcgc cgggtttggt tcgcaaatca
      361 gcatgacctt gattcgctgg attccaacgg ctgacggtat gcaccgcgcc cctgaagcac
      421 tgggctatga atatcgcgtc tatgatcgca ctcagttaaa tcagtggctg agcaccattt
      481 cgggctatcc gaccacacag cttgaagtga tcaaccggac gttgcgaatc aaagaccagg
      541 gcgctccggc attgccattg acaaccgccg ttccaaaacc tgtggttgcc gcgcctgtga
      601 cgtcctgggc acagccagcc gctgttccaa cgccagttgc cataccacca gccccagcca
      661 aggtcgagcc ggccacagtg gtggccaaag ttgaagcacc accagccgca ccagccttta
      721 cttcagtacc aacaccagtt ccagtctttg atgccgtgaa ggaaaaagtc ctttcactgg
      781 cagcgcaaaa gaccggctat ccggtggata tgctcgattt ggacctggat ctggaagctg
      841 acctgggcgt ggacacggtc aaacaagccg aaatgtttgc ggccatccgc ggcgcctatg
      901 acattccacg cgaagaaaat ctcaaactgc gtgacttccc gaccctcaaa cacgtcatca
      961 agtttgttta tgatcgccgc ccagatttga aagcagttga actgccgcca ctcccgacaa
     1021 aggtggtcgc gatggcatcc ggcgttggtg ccgcaccagc tctggcacca gtcatcgcgg
     1081 tggcaacgat tgatccagtt cgcgaagaag ttctgaaact gacggctcaa aaaaccggct
     1141 acccgattga catgctggat atggacctcg atctggaagc cgatcttggc gttgacaccg
     1201 tcaagcaagc cgaaatgttt gcggccattc gcggcgcata taccattcca cgtgatgaaa
     1261 acttgaaact ccgcgacttc ccgaccctca aacacgtggt caaatttgtc tatgatcgcc
     1321 gcccagacct gaaagctcca gagccagccg tcgccgcagt ggccgtgact gcccaggcac
     1381 cagtcgccac gccagccgcg ccggcggtgg atgaagtcac ccaaaaggta atggctctgg
     1441 ctgccgaaaa gaccggctat ccaccggaga tgctggacct tgatctcgat ttggaagccg
     1501 acctgggtgt ggacaccgtc aagcaggctg aaatgtttgc ctcggttcgc gcaatgtttg
     1561 acattccacg cgatgaaaac ctgaaactgc gtgacttccc aaccctcaaa cacgtgattg
     1621 ggtttgtcta cgccaaacgt ccggatctga aagtcgtcgc ggcaccagca ccggttgcca
     1681 cagctccagt tcaggtcaca gccccggcag ccgctccagc cgcgccggtt atggacgaag
     1741 tcaccgaaaa agtcctggct ctcgcggcgg acaagaccgg gtatccatcg gaaatgctcg
     1801 atctggacct cgatttggaa gccgatctgg gtgtggacac cgtcaagcag gccgaaatgt
     1861 ttgcctcggt tcgcgcaatg tttgacattc cacgtgatga aaacttgaaa ctgcgtgact
     1921 tcccaactct caaacacgtg attgggtttg tgtatgacaa acgcccggac ctgaaagtcg
     1981 ccgcggcacc agcgccggtt caaccagttg ccgctccggt cgccgaagca gcgccggcag
     2041 ttgatgaagt cacccagaaa gttctggatc tggccgtcga gaaaaccggg tacccagccg
     2101 aaatgctcga tttgaacctg gatctggaag ccgatctggg cgtggacacc gtcaagcagg
     2161 ctgaaatgtt tgcctcggtt cgcggactct ttgacattcc acgcgatgaa aatttgaaac
     2221 tgcgcgactt cccaaccctc aaccatgtga ttgggtttgt gtatgacaaa cgcccggatt
     2281 tgaaaggtgc cgccgcaacg caagccactg cggcaacagc aggtgccgcc gcatcggcgg
     2341 tcgcagcacc cgcagtacca caaaccatgt cgctggatgc ggctgccgct attccacgac
     2401 gcgtcccggt tccgatcctg cgaccggcac tggctgattg caaaccaacc ggcgtcaagc
     2461 tcgaagccgg cactcgcgtg gtggtcatgc ctgaactggg tgccg
//
LOCUS       DAOQDO010000816         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36587_length_2505_cov_2.915918, whole genome shotgun sequence.
ACCESSION   DAOQDO010000816 DAOQDO010000000
VERSION     DAOQDO010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36587_length_2505_cov_2.915918"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PKZ53_13135"
     CDS             complement(<1..1268)
                     /locus_tag="PKZ53_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41430.1"
                     /translation="MSVGMFAAGAGWTIAHADWQKARVQPGQKKSKAPRLETQSNTAH
                     GAKSPASTVKSRSTRQSSQADFPPVKLQELPGYGQKTIAGIPGVAIRAAEVDFTELAL
                     EEARQPLARRPKIEREKGPVPLDVIRPDEIVVPSPPATSVTPGTKALAPSPTPSSSFL
                     GLEDNGPSFPPDTHGAAGPNHLMVTLNTEIRIQDRQGKALRTVSLDGFWSAIGNPNSF
                     DPKVLYDPYQNRWMMTSTADSFSPTSSVLIGVTQTDDPTGKWNLYNIDADSTDVAWAD
                     YPSFGFNKDWIVVTVNMFGNESGGFRGTNAFVFNKADLYARGTGRYTLLQTPAFTLTP
                     AITHSDTLDTIYLVQNVGSGLRLYSITGSVGAEQLNTLSIVSTGDGWSSSEPGFEDSA
                     PQKGTTRKISANDGRIQNVVFRNGTIWATHT"
     gene            complement(1471..>2505)
                     /locus_tag="PKZ53_13140"
     CDS             complement(1471..>2505)
                     /locus_tag="PKZ53_13140"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ41431.1"
                     /translation="VRVTAADPVNNRASDASDANFTISRPALSPPTELSAAINGPQVR
                     LSWKAAANTPGASLTGYSIYRSRTSPVAAIGSNRLGTFPASLLAFSDRPNPADGKTQF
                     FYVVTAVYNTGESTPSNEVSAIPEPNNDTTPPQVTVIAPNGGETVNNGEPLQIRWQSS
                     DPGGGVASHNVDLSLDGGTTFGTSVAAGLGATAQSFVFQIPTTIASATARIRVTARDA
                     GGNNGTDSSDANFTIKSSDASPPTVTITSPTAGQKAKITASAGFNVTWSTADNVGVTS
                     QEVFLAKDGTTFQSLASGLSGSTTSFTLTQALAGPKGKNKAAKVRVVAKDAAGNSGTG
                     DSGAFKLIVK"
BASE COUNT          529 a          658 c          701 g          617 t
ORIGIN      
        1 gtgtgggtgg cccagatggt gccgttgcgg aaaaccacat tctgaatccg gccatcgttg
       61 gccgagattt tgcgtgtagt gcctttttga ggggctgaat cttcaaagcc tggctctgaa
      121 gatgaccacc catcgccggt gctcacaatc gaaagtgtat tgagttgctc ggcgccgact
      181 gagccagtaa tcgaatacag gcggagtcca gaacctacat tttgaaccag atatatggta
      241 tcgagggtat cactgtgtgt aatggctggc gtcagtgtga aggccggggt ttgcagcaag
      301 gtataccggc cagtgcctcg cgcatacaaa tccgctttgt taaacacaaa cgcgttggtc
      361 cctcgaaacc caccgctttc atttccaaac atattgaccg tgaccacaat ccagtctttg
      421 ttgaatccaa aacttggata gtccgcccag gcaacatctg tcgaatcggc atcaatattg
      481 tagagattcc attttccggt tggatcatcc gtttgggtta cgccaatcaa gaccgatgac
      541 gtaggcgaga acgagtcggc agtgctcgtc atcatccagc ggttttgata ggggtcgtac
      601 aacacctttg gatcaaagga gttgggattt ccgatagcac tccagaatcc atcgagggaa
      661 accgttctca gggcctttcc ctggcggtcc tggattcgaa tctcagtatt gagcgtcacc
      721 atcaaatgat tggggcccgc ggcgccatgc gtgtcaggcg ggaacgaagg gccattgtct
      781 tccagtccaa ggaaactgga agaaggtgtt ggcgagggcg ccagggcttt ggtcccaggt
      841 gtcacactcg ttgctggagg actggggacc acaatttcat ctggtcgaat gacatcaagc
      901 ggaacgggtc ctttttcacg ctcaattttt ggccgacgtg ccaggggctg tctggcttct
      961 tcaagcgcaa gctcagtaaa atccacttcg gcggctcgaa tggcaactcc aggaattcca
     1021 gcaatcgttt tctgaccata acccggtaac tcctgcaatt tgactggcgg gaagtcagcc
     1081 tgagacgact gccgggttga cctggatttc acggttgaag ccggtgactt tgcaccgtga
     1141 gcggtgtttg actgggtttc aaggcgcggt gccttcgatt ttttttgacc cggttggact
     1201 ctggcctttt gccagtcggc gtgagcaatc gtccaaccag ccccggcagc gaacatcccc
     1261 acactcatga aaatgagtgc aagatgagca aaacaaatac ctttcctcat gtgtgtacaa
     1321 ctccctggtt cagaatacag gtgttctacc acccttatcc tggtgtcgat gtgtcgtttt
     1381 tgaaactgaa ttggattgaa gagaatgaag tcgcgaagga cgtgtcagta ccacccactg
     1441 acgtaggtgg tactgactca ttggttcaag ttacttcaca atcagcttga aggcgccgct
     1501 gtcacccgtg ccgctgttgc cagccgcatc tttggcgacc acccgcacct tggcggcctt
     1561 gtttttgcct tttggtcctg ccagtgcctg cgtcaaggta aatgacgtcg tgctgcctga
     1621 caaaccgctc gccaggcttt ggaacgtcgt tccatccttg gccagaaaga cttcctggct
     1681 ggtgactcca acattgtcag ccgttgacca ggtcacgtta aagccggcgc tggctgtgat
     1741 tttggctttc tgtccggcgg ttggtgacgt gatggtcacg gttggcggac tggcatcact
     1801 cgatttgatc gtgaaattgg catccgaact atctgtgccg ttgtttccac cagcatcccg
     1861 ggccgtgacc cgaattcgag ccgtggctga ggcaatcgtg gttgggatct ggaacacaaa
     1921 cgactgggct gtcgcaccca aaccagcggc aaccgatgtt ccaaaggttg ttccgccatc
     1981 gagcgacaga tccacgttgt gagaagccac accgcctccc ggatctgacg actgccaccg
     2041 aatttgcaac ggttcgccat tattgacggt ttcaccaccg tttggtgcaa tgacagtcac
     2101 ctgtggtggc gtcgtgtcgt tgttgggttc aggaatagct gaaacttcat ttgacggagt
     2161 actttccccg gtgttgtaca ctgcggtcac aacatagaag aactgggttt tcccgtccgc
     2221 cgggtttggc cggtcgctaa acgccagcag cgatgccgga aatgttccca gtcggtttga
     2281 cccaattgcg gccacaggtg aagttcgcga acgatagatg ctatagccgg tgagcgaggc
     2341 cccaggggtg ttggcggcgg ctttccagct caaccggact tgtgggccgt taatggcggc
     2401 actcagttca gttggtggtg acaatgctgg tcgcgaaatc gtgaaattgg catcgctggc
     2461 atcgctggcc cggttgttga ccggatcagc cgctgtgacc cgcac
//
LOCUS       DAOQDO010000817         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36696_length_2501_cov_3.051104, whole genome shotgun sequence.
ACCESSION   DAOQDO010000817 DAOQDO010000000
VERSION     DAOQDO010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36696_length_2501_cov_3.051104"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>2501)
                     /locus_tag="PKZ53_13145"
     CDS             complement(136..>2501)
                     /locus_tag="PKZ53_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ41432.1"
                     /translation="FYTTGASVSSPSWFVLDTGQPPMIHGNIEFDRNTGQVNDFLNIL
                     SFHYQAARNQAVYPPTVETLDTLGVPLLSDAFAFERRETGIQLFRRGSSFLEFLKIGL
                     GPIQTPNTPVTERIGDLMFGVDYPRAFKLTTQATFLKALKNPEIRYIDFYNPSFDKHI
                     HDDIDPQSILGQLQELDTMLGQTYAALVESGSLDRTILVVVSDHGTTFDSQGRYSQGI
                     NLVNYLRRSEFGGHHVLTKFGTLSAYSLAGSILSPKVPPAAVSKSKESPYLGEKPAQV
                     TCAVDHDGNQQALIHFRHPDLNRLQILRSQAQKQSPSSPLWPQLEKQTREILTRAQPT
                     WAIQAGQIKQELAALRQVAQQTRSELEQIEPQVKAIKKRKGTPPTFANEADRFHSRSR
                     LHTLNPELDVLQHHKELTVVKFQTEGLIKEYQVVAGALDLLAGATSLAELVKLPEARL
                     FPPRMLGDHLEYQDLVKYPIGFQLIGNHDAAPTPGFQTINYLEALCGLQVKNSVRSDI
                     GTHPVEFVTCRMPARQVAAALVRAGYIPSEKAQNLSQAWLLYGGADQQVLEVALNGEV
                     PGGEVAYALFPVRKISLSSSNNSQIEIEFGDWKANLPLKLFEDPALMIHPEARAKWLS
                     AFHSGREWLTAVHLTEEGLGVLNLPQVLNDTYRQRLNAQIQATTDPVQKKWLEFQLRR
                     REAVEPDLFLHANKHWNFDSRDFNAGSNHGGFFRESMQSTLWLFGGEATPLRPGPVVV
                     HPPYEALDFVPTLFFALGYSHTGKVLPDFQKAGYGPFPGRVIEEIFR"
BASE COUNT          568 a          640 c          670 g          623 t
ORIGIN      
        1 aaaagtggag cttcctcgcc gtggcaaccc acccgctcac gcaggtggta ctggcttgac
       61 gccatccttt tttcggtggc ccggttttat ctccgataat atctaatgta tcaaaaacag
      121 gctctgtatt ctttctcacc gaaagatttc ttcaatgacc ctgcccggaa atggaccata
      181 cccggccttc tgaaagtcag gcaaaacctt gccagtgtgt gaatacccca gagcaaaaaa
      241 taatgttggc acaaaatcca gggcttcata cggtgggtgt accacgactg gccctggtcg
      301 aagcggggtt gcctcaccgc caaatagcca caaggtagat tgcatggatt cccggaaaaa
      361 tcctccgtgg tttgaaccgg cattaaaatc acgcgaatca aaattccagt gtttgttggc
      421 gtggagaaac agatcgggtt ccaccgcttc tcgtcgtcga agttgaaatt ccagccattt
      481 tttttgaact gggtccgttg ttgcctgaat ctgggcgttg agtcgctggc gataggtgtc
      541 attgagaacc tggggcaggt tgagcacgcc caatccttcc tccgttaaat gcaccgccgt
      601 cagccattcg cgacctgagt gaaaggctga aagccatttt gctctggctt cagggtgaat
      661 catcagggct ggatcttcaa agagtttgag cggcaagtta gctttccagt cgccaaattc
      721 aatttcgatt tgggaattat tgcttgatga aaggctgatt ttccgaaccg gaaacaacgc
      781 atatgccacc tcaccgcccg gtacttcacc gttgagtgca acttcgagca cttgttgatc
      841 ggcaccgcca tagagcagcc acgcctgtga caggttttga gccttttcag aaggaatgta
      901 cccggctcgc accagggctg ccgcgacttg cctggctggc attcgacagg tcacaaattc
      961 gacgggatgg gttccgatgt cagaacgaac cgaatttttg acctgcaacc cacacagcgc
     1021 ctccagatag tttatggtct gaaagccagg tgtcggagca gcatcgtggt ttccaatcag
     1081 ttgaaatcca atggggtact tcaccaaatc ctgatattca aggtggtcac ccagcattcg
     1141 cggagggaac aacctggcct caggcagttt caccagttcc gccagcgatg tggcacctgc
     1201 cagcaggtcc agggccccgg caaccacctg gtattctttg atcaagcctt cggtttgaaa
     1261 tttcaccacg gtgagttctt tatggtgttg caggacatca agttctggat tcagggtatg
     1321 gagtcgcgaa cggctgtgga atcggtctgc ttcattcgcg aaggttggtg gggtgccctt
     1381 tcgctttttg atcgccttga cctgcggttc gatttgctca agctccgacc gggtttgttg
     1441 cgccacctgc cggagcgccg ccagttcctg cttgatctga ccagcctgga tggcccaggt
     1501 tggttgggca cgtgtcagga tttctcgggt ttgtttctca agctgaggcc agagcggact
     1561 tgatggagac tgcttctggg cctgagaccg caggatttgc agccgattga ggtccgggtg
     1621 acgaaagtga attaacgcct gctgattgcc gtcatggtcc actgcacagg ttacctgtgc
     1681 cggcttctcc ccgagatagg gtgattcctt tgattttgag actgccgccg gaggaacttt
     1741 cgggctcaag atcgaacccg ccagcgaata ggccgaaagc gtgccaaatt tggtcaatac
     1801 atgatgacca ccaaactcgc tccgacgaag ataattgacc agattgatgc cctggctata
     1861 gcgtccctga ctgtcaaacg tcgtgccatg atcggaaacc acgaccagta tggttcggtc
     1921 gagtgacccg ctttccacca gtgcggcata cgtctgcccc agcatggtgt ccagttcctg
     1981 aagctgtcca agaatgctct gtgggtcaat gtcgtcgtgg atatgtttgt caaagcttgg
     2041 gttatagaaa tcaatgtacc tgatttccgg atttttcagt gctttgagaa atgtggcctg
     2101 cgtggtgagt ttaaacgccc gtggataatc aaccccaaac atcaaatctc cgattcgttc
     2161 ggtgacgggt gtgtttgggg tctggatcgg cccaagtcca attttgagaa attcaaggaa
     2221 acttgaacct cgtcgaaaaa gctgaatccc cgtttcacgg cgttcaaacg caaacgcgtc
     2281 tgaaagcaac ggcaccccaa gggtatcgag ggtttcgacc gttggcggat agaccgcctg
     2341 attgcgtgcc gcctgataat gaaagctgag aatattcaga aaatcgttga cctgcccggt
     2401 atttcggtca aattcgatgt tgccgtggat cattggtggc tggccggtat cgagcacaaa
     2461 ccatgacgga gacgagaccg aagccccggt ggtgtaaaaa t
//
LOCUS       DAOQDO010000818         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36697_length_2501_cov_2.892478, whole genome shotgun sequence.
ACCESSION   DAOQDO010000818 DAOQDO010000000
VERSION     DAOQDO010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36697_length_2501_cov_2.892478"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKZ53_13150"
     CDS             <1..411
                     /locus_tag="PKZ53_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41433.1"
                     /translation="EASKLGTWSEARTKFFEFAKLDLETQARIYLEEQNWWEALRVAQ
                     SAQHHESLKVLVADGIKHHLPNEAMAIYEKLVTDYIGRQKRESYKSAATYAEKMKDIY
                     LTVLKDQTTWKTWIAKLREENKRRPALQDEFRKL"
     gene            complement(590..1339)
                     /locus_tag="PKZ53_13155"
     CDS             complement(590..1339)
                     /locus_tag="PKZ53_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ41434.1"
                     /translation="MNGKPNPPNCNHKDDLVTYLYGEATPAESRAFEHHIKECASCRE
                     ELEAFTGVRRVMQTWEVETVPRIEIDVRPTFWQTLKQFFVVTPVWGKVVMAGACGLVL
                     LACLNVRVAIGRDGVTFSAGFQPAPQVVETRILPAKPVEVQPTLQNPTPTTVAQYDEK
                     AIRVLVEELIKASESRQDGKLAAQFEVLATQLKARNDAELIKAVSALKREQRQQLLNV
                     LHEVDRQQQAPDLLDLLGQAQTRDTSTDLEP"
     gene            complement(1502..2068)
                     /locus_tag="PKZ53_13160"
     CDS             complement(1502..2068)
                     /locus_tag="PKZ53_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41435.1"
                     /translation="METTDAELVERVLLGETEVFGILVRRWERPIYSLAVRMLGRDED
                     AYDICQETFLSAFRNLNSFRGEAKFSSWLYRIAINCCHSRLRKHSEKANTSIDEQVEE
                     HGLEPSDHRVDLHDELYRREAADLVRKALAALPPEMRQVIVMKEYEGLKFHEIADILG
                     VPVSTVKTRLYTGLTYMRKRLEHVKDAL"
BASE COUNT          631 a          620 c          645 g          605 t
ORIGIN      
        1 gaagcttcca agctgggcac ctggtcggaa gctcgcacga aattctttga attcgccaaa
       61 ttagacttag agactcaggc ccggatttat ctggaagagc aaaactggtg ggaagcgctc
      121 cgcgtggccc aatcagccca gcatcacgaa tcgctcaaag ttctggtggc agatggcatc
      181 aaacaccacc tccccaacga agcaatggcc atctatgaaa aactggtcac ggattacatt
      241 ggccgccaga aacgtgaatc ttacaaatct gcggcgacct atgctgaaaa gatgaaagat
      301 atttacctga cggtgttaaa ggaccagacc acctggaaaa catggattgc aaagctgcga
      361 gaagaaaaca aacgacgtcc ggcactgcag gatgaattcc ggaaactgtg aaaaaccagg
      421 gctgaagaag acgggcttag ggatgaagac tcgcaagctc ggggttgaag aaaacaagtt
      481 aaagaacatt gggctgaaga agttgggatt gacaattcaa tatccttttt cttcagcccc
      541 aagccctcag cccaatgtct tcagcccgcc ttcttcagcc ctggttttat tacggttcca
      601 ggtcagtcga agtatcgcga gtctgagcct ggccaagcag gtcaagcagg tcgggtgcct
      661 gttgctggcg gtcaacttcg tgcaggacat tgagcagttg ctgccgctgt tcccgtttaa
      721 gcgcactcac ggctttgatg agttccgcgt cgttacgcgc tttcaactgc gtagccagca
      781 cttcaaattg agcggcgagc ttgccatcct gacgtgactc actggccttg atgagttctt
      841 caaccagaac ccgaattgct ttctcgtcat actgggcaac cgtggttggg gttggattct
      901 gcaatgtggg ttgaacttca actggtttgg cgggcagaat acgggtttca accacttgcg
      961 gggccggttg gaacccagcc gaaaacgtaa cgccatcacg gccaatggct acccgaacgt
     1021 tcaaacaggc cagcagcacc aatccacagg cgccagccat caccactttg ccccacaccg
     1081 gtgtcaccac aaaaaactgt ttgagggttt gccaaaaggt aggccggaca tctatttcaa
     1141 tgcgcggcac ggtttcgact tcccaggttt gcatcactcg ccgcacacca gtaaaggctt
     1201 ccagttcctc tcgacaggaa gcgcactctt tgatgtggtg ctcaaaggct cgactttcgg
     1261 caggtgtcgc ctcgccatag agataggtca caaggtcatc tttgtggttg cagtttgggg
     1321 gatttggttt gccgttcatg gtaggtctcc gaactctttc agggtgggat tggattgttc
     1381 gtttcgatct aaattattga aataaaagat agtatccagt cagtagtcag tagttcgcta
     1441 agttcatttg gttaaatcac ttgaagatat caaaataaga aatactcatt ttaaaatagc
     1501 attacagagc atctttgacg tgttccagcc gtttgcgcat ataggtcaat ccggtgtaaa
     1561 gccgggtttt caccgtgctg acgggaacgc ccaaaatatc ggcaatttcg tgaaatttca
     1621 gtccttcata ttctttcatc acaatcacct gccgcatttc cggagggagg gccgccaggg
     1681 ccttgcgaac cagatcagcc gcttcccgcc gatacagttc atcgtgtaaa tcaacccgat
     1741 gatccgatgg ctccagacca tgctcttcaa cctgttcatc aattgaggta ttggcttttt
     1801 cggaatgctt tcgcaatcgc gaatggcaac agttaatcgc aatccgatac agccaggatg
     1861 aaaatttggc ctcacctcga aaagaattga gattgcgaaa cgctgagaga aaggtctcct
     1921 ggcagatgtc ataggcatct tcgtcccgcc caagcatacg cactgccagg ctgtaaatcg
     1981 gacgctccca ccgacgcacc aggatgccaa acacctcagt ttcgcctaat aagacacgct
     2041 caaccagttc agcatcagtc gtttccaagg ttcgtttctc ctcacttcgc atttacctcc
     2101 gggatgtggt gttttcggtg gcactgagtt ctttttgaat tctgggattg ttttttcagt
     2161 tgtgccttgt gttttgccag taagagccac gagtcagaaa aatagtttgg agaatttcag
     2221 ggttcagggg tcaggggtca gggttcaggg gtcagggttc agggttcagg gttcaggggt
     2281 cagggttcag ggttcagggt tcagggttca gggttttcga atcttcgtcg attatagttt
     2341 tcgccgcgta gcggtggttg aattgaggca ggtcgtttac ggcctgctgt aaaaccaggg
     2401 cttggggaaa agacaaaaag gacacaaagg acacaaagga ccaaagttcg aaaacccgga
     2461 acccggaacc cggaacccgg aacccggaac ccggaacccg g
//
LOCUS       DAOQDO010000819         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36733_length_2500_cov_2.655215, whole genome shotgun sequence.
ACCESSION   DAOQDO010000819 DAOQDO010000000
VERSION     DAOQDO010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36733_length_2500_cov_2.655215"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKZ53_13165"
     CDS             complement(<1..498)
                     /locus_tag="PKZ53_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41436.1"
                     /translation="MKHKAVFTKVRKLIDQREVVEENLDFAEDALVELVEKDPNCDWA
                     LGLLSEIYYWRGEVAPASEKLELYQQGVEYGEQGVELNENSLEANFWLGVNYGLYGAE
                     KGVLKSLELIKPIQACMERALEVDERYFYGGPWRVLGRLYNKVPSWPISIGDNRKSIE
                     CLETAL"
     gene            complement(603..1133)
                     /locus_tag="PKZ53_13170"
     CDS             complement(603..1133)
                     /locus_tag="PKZ53_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41437.1"
                     /translation="MHRKQVQCLIVVGLWMLVTTVSVHSEAGKLPSVIKTSVDSVPPT
                     TSKDELLNWLKAEKYKAEFIAEPEVHPSSGPHGGNVRTYFNSILTEDLRAGKTRFRKN
                     AAMVKELYFGGTETVIGYAVMVKVKKKSKQNGKEWLYYETFDGTNGSVFYGRGVRLCV
                     DCHQAGTDYLLSPFRP"
     gene            1536..>2500
                     /locus_tag="PKZ53_13175"
     CDS             1536..>2500
                     /locus_tag="PKZ53_13175"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNJ41438.1"
                     /translation="MALSRSKKILVIGGIVVLLGGITAASVLSRNRNAVPVQFDTVKR
                     FEILESKVSASGEIRPKELYNLTAEVSGRVTSIKVTEGQLVKKDQPLIEVDPTQQATA
                     KASAEANYQAAIQEKLQVQQQLFSAENNVNQTKNLLAGAQADLRRARATQDLNQKELN
                     NLSKLVETGIVSKIEIDRASANLETAKAAVEAQEEQVKRLQTQVKDAEISVDRMKAAL
                     AGSEQREKSSKANLDSANDILNKTIKLAPIDGVVASLPVRVGEFALANLSSTPLMTIA
                     DMSEIFVEVKVDETDIANVKAQPQQSAKVKVDALNNIEIDGFVVEV"
BASE COUNT          724 a          628 c          545 g          603 t
ORIGIN      
        1 gagagccgtt tcaagacatt caatagattt gcggttgtca ccaatcgaga ttggccagct
       61 tgggaccttg ttatacaacc gaccaagaac tcgccagggc ccgccataaa aatagcgctc
      121 atccacttca agggcccgtt ccatgcaggc ttgaattggc ttaatcaatt ccagactttt
      181 taaaacgcct ttttcagcgc catagagccc ataattgaca ccgagccaga aattggcctc
      241 aagtgaattt tcattcagtt caacgccttg ctcgccgtat tcaaccccct gctgatacaa
      301 ctccagtttt tcgcttgccg gtgccacctc acctcgccag taataaattt ccgacaaaag
      361 tcccagggcc cagtcacagt tgggatcttt ttccaccagt tcaacaagtg catcttcagc
      421 gaaatcaagg ttttcctcga ccacttcacg ttgatcaatc aatttgcgca cttttgtaaa
      481 aacagccttg tgtttcattc gttcgtccta attcgaaaaa tcaaaatacc cgcgccgggt
      541 gcagtgtatg ctttgaaagg gatgatggaa attgtaattg agacggatgg gaaaccacct
      601 tgtcaaggcc gaaatgggga gagcaaatag tcagttccag cctgatgaca gtctacgcag
      661 agtcgaaccc cgcgcccata aaaaactgag ccgttggtgc catcaaaagt ttcataatac
      721 agccattctt tcccattctg cttgctcttt ttcttcactt tgaccatcac agcatagcca
      781 atcaccgtct cagttccacc gaaatagagt tccttcacca tagccgcatt ttttctaaac
      841 ctggttttcc cagcgcgcaa atcctcagtt aaaatcgagt taaagtaggt tcgaacgttt
      901 ccgccgtgtg gtcctgacga tggatgaact tcaggctcag caataaattc tgctttgtat
      961 ttttctgcct tgagccagtt taacaactca tcttttgatg tcgttggcgg gacagaatct
     1021 acagaagtct tgataacaga aggaagtttg ccagcttcag agtgcacgct gaccgttgtc
     1081 accagcatcc aaagaccaac cacgataagg cattgaactt gttttcgatg cataaacaca
     1141 actccttatt caaaattttg gaaagataaa ccgggttgaa ttcagtaaaa cggggttgca
     1201 tcagcttcaa ggacttcagg tatgatcaca gattaccatt tttcagcatt tttggatgcc
     1261 aggagactga gaactggacc ctagtcccac gctctatccc tggttgcaaa caaacgaaag
     1321 cgttctggtc catcattccc tgcactgccg agtaaaaggt tcattcaact gatcttggaa
     1381 agaggcacca ggggtttggt tcaaggaaaa ttttcaagaa aatgtaaaag attgcacgaa
     1441 acttgtctgg aaaggtagac gtagaaggct cacaactcac actccaaaca gtcttcacat
     1501 catccaaaaa attcaataat cgagaggcag tcacaatggc attaagtcgt tccaagaaaa
     1561 tccttgtcat cggagggatt gtggttctcc tcggcggaat caccgccgcc agtgttctca
     1621 gtcggaaccg aaacgcagtt cccgttcagt ttgataccgt caaacgattt gaaatacttg
     1681 aatcaaaggt ttctgcctct ggcgaaattc gccccaaaga gctgtataac ctgacggcag
     1741 aagtttcagg ccgggtcacc tcaattaaag tgactgaagg ccaactggtc aaaaaggatc
     1801 aaccgttaat cgaagttgat cccacccagc aagccactgc caaagccagt gctgaagcca
     1861 actatcaggc cgcgattcaa gaaaaactcc aggttcaaca acaactcttc agcgctgaaa
     1921 acaacgtcaa tcaaaccaaa aatctgttgg ccggcgccca ggctgacttg cgtcgggcac
     1981 gggcaaccca ggacctcaac cagaaggaac tcaataacct gtcaaaactg gttgaaactg
     2041 gcatcgtttc caaaattgaa attgaccggg cgagtgccaa tttagaaacg gccaaagcag
     2101 cggttgaagc ccaggaagaa caggtcaagc ggctccaaac ccaggttaaa gatgctgaaa
     2161 tcagtgttga tcgcatgaaa gccgccctgg ctggaagcga gcagcgtgaa aagtcatcca
     2221 aagccaacct cgatagtgcc aatgatattt tgaacaaaac catcaaactg gccccgattg
     2281 atggcgtggt ggccagcctt ccggtccggg tcggtgaatt tgcgctggca aacctgagca
     2341 gcaccccgct gatgaccatt gccgatatgt cagaaatctt tgtcgaagtc aaagttgatg
     2401 aaacggacat cgccaatgtg aaagcacaac cacagcagtc agccaaagtc aaagttgatg
     2461 cgctgaataa cattgaaatt gatgggtttg tggtcgaagt
//
LOCUS       DAOQDO010000820         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36805_length_2498_cov_2.524355, whole genome shotgun sequence.
ACCESSION   DAOQDO010000820 DAOQDO010000000
VERSION     DAOQDO010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36805_length_2498_cov_2.524355"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="PKZ53_13180"
     CDS             <1..1843
                     /locus_tag="PKZ53_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ41439.1"
                     /translation="GSYALVIGASEYRNGWDRLPGVKQDVSAVKETLESQGFEVRVVL
                     DPTRENFDRAMKQFIDDYGYVEKNRLLIYFAGHGHTEKGSDDRVFGYIVPVDAPLPDR
                     DRVGFKRSAVSMDAIEAYARLIETRHALFVFDSCFSGSLVSRSTVVPPEISDATAKAV
                     RQFITSGAANQEVPDRSVFRDLFVRGINGAADLNGDRYVTGSELAMYLKSQVTEYSRG
                     AQTPQYGKIRDTKLDLGDYVFMVTGALKPPPKMEVITEPVPVGTLRVTAFNKGMEVYV
                     NGEKAGTAAYGGQKFTIKNVPARGTVEIRGRTGDLETVKRVAVGEGQETPVELMFDRI
                     VNSLGMELKFVPGGTFMMGCTAEEADRAWGLFKQGYPDTPKEWFTNATPRREVTISQG
                     NYVGMFEVTQEQWKRVMGTAPSYFQSPKYADCPDCPVEQVSWDEVQEFIKKLNELEGC
                     NGCYRLPTEAEWEYYARAGTTGDYAGDLDRLAWYGNNAGNKVFDAQALWAQYKSAGYM
                     TKILENGNRTHSIVETKQSKQPNGFGLYNLHGNVWEWCEDWYGSDSYQKGPSVDPRGP
                     SGGSSRVMRGGGWSSSAVHCRSAYRDFHDPGFRLIILGFRLLRIADR"
     gene            complement(2005..>2498)
                     /locus_tag="PKZ53_13185"
     CDS             complement(2005..>2498)
                     /locus_tag="PKZ53_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41440.1"
                     /translation="KTMETKGEAETRREWKFRIVRSLYERGYAREDVRKLFRLVDWFM
                     KLPPEVETKFQKDLEEYEEQHHMPYVTAIERMAEARGKAEGKAEGKAEGKAEGKAEGR
                     LLSKIETLFLTLETRFGDVAPDAAATIQAIDDEAFLTRLQKKAILAATIEEFYEFLQK
                     QDG"
BASE COUNT          570 a          608 c          758 g          562 t
ORIGIN      
        1 cggcagttat gcgctcgtca ttggcgccag cgagtatcgc aatggatggg accgacttcc
       61 gggggtgaaa caggatgttt ctgccgtgaa agaaacactt gaaagtcaag gatttgaagt
      121 cagggtggtc cttgatccaa cgcgggaaaa ttttgaccgg gccatgaagc agtttattga
      181 cgattatggg tacgtggaga aaaaccggtt gctgatctat tttgccggtc atggtcacac
      241 ggaaaaagga tcggatgatc gggtcttcgg ctatatcgtt ccggtggatg ccccgttgcc
      301 agatcgggat cgagtggggt tcaaacggtc agccgtcagt atggacgcca tcgaagccta
      361 tgcgcggttg atcgaaaccc ggcatgcgtt gtttgtgttt gacagttgtt tttcaggatc
      421 gctggtgagc cgctccaccg tggtgccgcc ggaaatcagc gacgccacgg ccaaagcggt
      481 tcgccagttt atcacgtcgg gtgcggccaa ccaggaagtt ccagaccgga gcgtcttccg
      541 ggatttgttt gtgcgcggga tcaacggtgc cgctgattta aacggagacc gatatgtgac
      601 cggaagcgaa ctggccatgt atttgaaatc acaggtcacg gaatattcac gcggggccca
      661 gacaccgcaa tacggcaaga ttcgggatac caaactggat ctgggagact acgtgtttat
      721 ggtgaccgga gcgcttaaac caccgccaaa gatggaagtc atcaccgaac cggttccagt
      781 cggtacactc cgggtgacag ccttcaacaa aggcatggaa gtttatgtca acggcgaaaa
      841 agccggcacg gcagcctacg gtggccaaaa gttcacgatc aaaaatgtgc cggcccgggg
      901 aacggttgag attcggggac gcaccggaga tctggaaacc gtcaaacggg ttgcggtcgg
      961 cgaagggcag gaaaccccgg ttgaattgat gtttgaccgg attgtcaata gccttggaat
     1021 ggaattgaaa tttgtccccg gtggaacctt tatgatgggc tgcacggctg aggaagctga
     1081 ccgggcgtgg ggtttattca aacaaggata cccagataca ccaaaagaat ggtttaccaa
     1141 tgcaaccccg cgccgtgagg tcacgatcag tcagggaaac tatgtgggta tgtttgaggt
     1201 gacccaggaa cagtggaaac gggttatggg gactgctccc agctattttc aatcgccgaa
     1261 gtatgctgac tgcccagact gtccggttga gcaagtcagt tgggatgagg tgcaggaatt
     1321 catcaaaaaa ctcaatgagt tggaagggtg taatgggtgc taccggttgc caacggaggc
     1381 ggagtgggaa tattacgccc gggcgggaac cactggcgac tatgctggtg atttggacag
     1441 gctggcttgg tatggaaaca acgccgggaa caaggtgttt gatgcacagg cactgtgggc
     1501 acaatataaa agtgccggct atatgactaa gatactggag aacgggaatc gaacccattc
     1561 aatcgttgaa acaaagcaat caaagcagcc aaacgggttt ggactgtaca acctgcatgg
     1621 gaacgtgtgg gagtggtgtg aagattggta tggaagtgac tcctaccaga agggtccgag
     1681 cgtggacccg aggggaccga gtggaggctc gagccgggtc atgcggggcg gcggttggag
     1741 cagcagcgcc gtgcactgcc ggtcggcgta ccgcgacttc cacgaccctg gcttccgcct
     1801 catcatcctc ggcttccgcc ttttgaggat agcggatcgg tagcggtgtg cacttttaca
     1861 cttttaccat ttgccgggtg ggcgcgcggc gagcgagaac gaagctgagg agcgagggcg
     1921 aaagcgaagc cgcccgcgac gaagcgaaga gagagccgcc gcgcgaaaaa tttttttcgg
     1981 agtgcggctc acttgacgcc gctgtcagcc gtcctgtttt tgcagaaact cgtagaattc
     2041 ctcaatcgtc gccgccagga tcgctttctt ttgaagccgg gtcagaaagg cttcatcgtc
     2101 aatcgcctgg atggtcgcgg cggcatctgg ggccacgtca ccaaaccggg tttcaagggt
     2161 caggaacagg gtttcaattt tcgagagtaa tcggccttcg gctttgcctt cggctttgcc
     2221 ttcggctttg ccttcggctt tgccttcggc tttgcctcgg gcttcggcca tgcgttcaat
     2281 cgcagttaca tatggcatat ggtgttgctc ctcatattct tccaggtctt tctggaattt
     2341 ggtttccact tccggcggta acttcataaa ccagtccacc agccgaaaca gtttgcgcac
     2401 atcctcacgt gcatatcccc gttcgtagag tgatcgcacg atgcgaaatt tccactcccg
     2461 gcgcgtttcc gcttcacctt tggtttccat cgttttca
//
LOCUS       DAOQDO010000821         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36826_length_2497_cov_2.997133, whole genome shotgun sequence.
ACCESSION   DAOQDO010000821 DAOQDO010000000
VERSION     DAOQDO010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36826_length_2497_cov_2.997133"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKZ53_13190"
     CDS             <1..907
                     /locus_tag="PKZ53_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41441.1"
                     /translation="MKPHRKHLLLATDGELNLIPFSALIDEHQKFLVERYTVTNLTSG
                     RDLLRLQVKVDAHSPPMILADPDYATGNGPTLFDKQLKPLSRLIGTKQEARIIQTELP
                     EFVVKLGAEATKSVLESVDQPKLLHIATHGYFLENAGPSFVGTTDQRSLAALTDTPVD
                     FDQLKQNTPLLRSFLFLAEANKSEIGIMTAAETAQLNLWGTKLVVLSACETGLGDVKN
                     GEGVYGLRRALVLAGSEAQMISLWAISDRGTQELMAEYYHRLKAGEGRSQALRNTQLK
                     LLKDPKRQHPFYWASFIQSGEWRPL"
     gene            1138..1467
                     /locus_tag="PKZ53_13195"
     CDS             1138..1467
                     /locus_tag="PKZ53_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ41442.1"
                     /translation="MTHSLDTTFAALADPTRRAILARLALGEATVMELAQPFAMTQPA
                     ISKHIKVLEKAGLISRGRDAQRRPCKIKPEALDEAMTWLEEYRRLWEANYERLDALLD
                     ELKRNQP"
     gene            1505..1996
                     /locus_tag="PKZ53_13200"
     CDS             1505..1996
                     /locus_tag="PKZ53_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ41443.1"
                     /translation="MKNTLQITTPSDREIVMTRAFDAPRHLVFDCFTKPELLKRWMTG
                     PDGWTFAVCEIDLKVGGRFRYVWRQVDGTEMGMTGEFREIVVPERIVNTEQFDQDCTG
                     GEALGTLVLTEQNGKTTTTNTVLYSSQTARDGALKSGVEDGVAAGYDRLDEILGSPLA
                     AQA"
     gene            complement(2147..>2497)
                     /locus_tag="PKZ53_13205"
     CDS             complement(2147..>2497)
                     /locus_tag="PKZ53_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017781428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ41444.1"
                     /translation="GGRIICTSTGLTRFAIPGYAAYASMKGAIEVFIKYLAKELGPRG
                     IAANVVAPGAIETDFTRSAFEHAGVKDFLASQTALGRVGVPDDIGGVVAFLCSEEGRW
                     VNAQRLEASGGMFL"
BASE COUNT          649 a          663 c          635 g          550 t
ORIGIN      
        1 catgaaaccg catcgcaaac acctcctgct ggcaactgac ggcgaattaa acctgattcc
       61 tttttcggcc ctgattgatg aacatcagaa attcctggtt gagcgctata cagtgactaa
      121 cctgacgagt ggacgtgacc tcttgcggtt acaggtcaaa gttgacgccc attcgccacc
      181 aatgattctt gcggatccag attatgcgac tggcaatggc ccaaccttgt ttgacaagca
      241 gttgaaaccc ttgtcaaggc tgattggcac caagcaggaa gcccgcatca tccagactga
      301 actccctgag tttgttgtca aactgggtgc tgaggcgacc aaaagcgtac ttgaatcagt
      361 tgatcagcca aaattgctcc atatcgcgac ccatgggtat tttttggaaa atgccgggcc
      421 gtcattcgtt gggaccaccg accagcggtc actggcagca ttgactgaca ctccagtgga
      481 ttttgatcag ttaaaacaaa atacaccgtt gcttcgctcg tttctttttc tggctgaggc
      541 gaataaaagt gaaattggaa ttatgacggc ggctgaaacc gctcaactca atctgtgggg
      601 caccaaactg gtggtgctct cagcctgcga gaccgggtta ggcgatgtca aaaacggaga
      661 aggggtctat ggcctgcgcc gggcgctggt tttagctggc agcgaagcgc agatgatcag
      721 tctatgggca atctcagacc gagggactca agagttaatg gccgaatact atcaccgtct
      781 caaagcgggg gaaggccgca gccaggcgtt gcgcaacacg caactcaagt tactcaaaga
      841 cccaaaacgc cagcacccat tctactgggc ttcattcatt caatccggcg agtggagacc
      901 gctgtaaaac cattctgggt tgaggacctg gggcaatcga agggatgagg gatgaaataa
      961 aatcaggtct tcagccccaa gccctcagcc ctcagcccgt tttcttcagc cctcagcccc
     1021 aaaccctgaa cccgatgtct tcagccccaa acgcaatgct ctcaaaatct acttgacgag
     1081 aaaatattac tagcgtatat tcctgaaagg ttatttaacc acctggttat ttctatgatg
     1141 acacattcgc ttgacaccac ctttgcggca ctggctgatc caacgcggcg ggcaatcctg
     1201 gctcgtctgg cattgggtga agccaccgtg atggaactgg cacagccatt tgccatgacc
     1261 caaccggcaa tctccaaaca tatcaaagtg ttggaaaagg ctggcttaat ctctcgtgga
     1321 cgtgatgccc aacgacggcc ttgcaaaatc aaacctgaag ccctcgatga agccatgact
     1381 tggctcgaag agtatcgccg actttgggaa gccaattatg agcggcttga cgccctgctc
     1441 gatgaactga aaagaaatca accgtgacca aaagaccgaa agaaagattt aaggagacga
     1501 ccgaatgaaa aatacacttc aaattacaac ccccagtgat cgtgaaattg tgatgacccg
     1561 cgcttttgac gctccgcggc atctggtttt tgattgtttt accaaacccg aattgctcaa
     1621 acggtggatg accgggcctg atggctggac gtttgccgtt tgcgaaattg atctcaaggt
     1681 tggtggccgc tttcgctatg tctggcgtca agttgacgga accgagatgg gcatgaccgg
     1741 tgaattccgg gaaattgtgg tgccggaacg cattgtcaac accgaacagt ttgaccagga
     1801 ctgcacgggt ggcgaagccc tggggacgct ggtccttact gagcaaaacg gcaaaacaac
     1861 taccacgaat acggtgctct attcatcgca aaccgcccgc gatggcgcgc tcaagtcagg
     1921 agtggaagac ggcgtggccg ccggatatga ccgacttgat gaaattctgg gttcacctct
     1981 ggctgcccag gcttaggcgc aaagaatatt gattggtaag ggggcgtcag tcgtttgctc
     2041 atctggtttg attgaatgac ttgaccctgg ttccatctca ggatttcagt ggccaaaagt
     2101 agagcgacat caaaaagtga gctgactact gacgcaaccc caatcttcac agaaacatcc
     2161 cgccggatgc ctccagtcgt tgggcattga cccagcgacc ttcttccgaa cacagaaacg
     2221 caaccacgcc accaatgtca tctggaacac ctacccggcc cagggccgtc tgcgaagcca
     2281 gaaaatcctt gactccggcg tgttcaaaag cgctccgggt aaaatcagtt tcaatggcgc
     2341 caggtgccac aacattggcg gcaataccac gtggccctaa ttctttggcc agatacttga
     2401 taaacacctc aatcgcgccc ttcattgagg cataggcggc ataaccgggg attgcaaagc
     2461 gggtcaagcc ggtcgaagtg cagataatgc gcccgcc
//
LOCUS       DAOQDO010000822         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36830_length_2497_cov_2.490172, whole genome shotgun sequence.
ACCESSION   DAOQDO010000822 DAOQDO010000000
VERSION     DAOQDO010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36830_length_2497_cov_2.490172"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..474
                     /locus_tag="PKZ53_13210"
     CDS             1..474
                     /locus_tag="PKZ53_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNJ41445.1"
                     /translation="MAIPVAGGAISVILQKNPLYGAFSLILSFLGLSAIYIMLGAHFL
                     GVIQIVVYAGAIMVLFVFVIMLLNVRREATHIDHHAYLKPLAGILGLVLLAQLVFVVA
                     RVAIPQSPTVSPGVGTVETVGKELLTTYLLPFEATSVLILMAIIGSMHLAKREKE"
     gene            655..957
                     /gene="nuoK"
                     /locus_tag="PKZ53_13215"
     CDS             655..957
                     /gene="nuoK"
                     /locus_tag="PKZ53_13215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNJ41446.1"
                     /translation="MVPLSWYLLLSAFLFTVGAVGVFVKRNVISVLLCLELMLNAANL
                     ALIAFSSFFQQADGQLFVFLVITVAAAEAGVGLAIVISVFRNRETLDVDESHLLKM"
     gene            1093..>2497
                     /gene="nuoL"
                     /locus_tag="PKZ53_13220"
     CDS             1093..>2497
                     /gene="nuoL"
                     /locus_tag="PKZ53_13220"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNJ41447.1"
                     /translation="MLKLIVLAPLVGSILLAFIGKRLSERLVGLLACSTVGISAITAF
                     YTFFTALLPAKEGADGIRRVTEHVATWMQVGTFKADFGLALDPLSGIMVLFVTGVGFL
                     IHVFATGYMHGDKSFHRFFCYLNLFMFAMLTLILADNFLLMFVGWEGVGVCSYLLIGY
                     YFLKQEAGDAAKKAFIFNRIGDFGVVLGVLLIFNNFGTINFAEVMEKAAQLPTEPFGM
                     AAVMAGGLTTIALLLFIGATGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVTAGVYM
                     VARCNAIYSKAPTALLVVAVIGGLTALFAATIGLGQWDIKKVLAYSTVSQLGYMFLAC
                     GVGAYTAAIFHVMTHAFFKALLFLGSGSVIHGMHHEQDMRYMGGLKKYMPVTRWTMIC
                     GWIAIAGIPPFAGFFSKDEILWKTFSTTVLPGDAGKILWGLGVVTALLTAIYMTRLMV
                     VTFEGGERYKESHGHDDH"
BASE COUNT          513 a          603 c          690 g          691 t
ORIGIN      
        1 atcgccatcc ccgtggcggg tggcgccatc agcgtcattc ttcagaaaaa cccgctctat
       61 ggggcttttt cactgattct gtccttcctt ggtctgtcag ccatttacat tatgctaggc
      121 gcccacttcc tgggggtcat tcagatcgtg gtgtatgccg gtgcgatcat ggtgttgttt
      181 gtctttgtga tcatgctgtt gaacgtgcgt cgcgaggcaa cccatattga tcaccacgct
      241 tacctgaaac ctctagccgg tatccttgga cttgttttgc tggcacagct tgtatttgtg
      301 gtggcccgag tggccattcc ccagtcgcca accgtcagtc ctggtgtggg aacggtcgag
      361 actgtcggga aagagttgtt gacgacctat ctgttaccgt ttgaagccac atccgtgctg
      421 attttgatgg ccattattgg ttcaatgcac cttgccaaac gagagaaaga atagctttct
      481 gggccaagcc gaagaaatca gaatgaagaa ttgagaattg agaatgaaga aaccagagta
      541 aaggattgag catgaaaagt tgaaaggttt gagctgctaa ctttcttcat tcttcattct
      601 ttattctcag ttcttcacta acccaaaaac ttccaacccc atacccaaaa aactatggtt
      661 ccactgtcct ggtatttatt gttaagcgca ttcctgttca cggtgggggc ggtcggtgtg
      721 tttgtcaaac gcaacgtgat ttcggttctg ttgtgtcttg aactgatgct caatgccgcc
      781 aatctggcgc tgattgcctt ttccagcttt tttcaacagg cggatgggca gctttttgtg
      841 ttcctggtga ttaccgtggc tgccgctgaa gccggtgtcg ggctggcaat tgtgatttcg
      901 gtgttccgca accgtgaaac actcgacgtt gatgaatctc atttgttaaa gatgtgatct
      961 gggtccacag tactttgtgc ttggtacttg gtgctttgta aatcctattt aatgaatcgt
     1021 ttaaatagag caagtgccaa aaagtaaaaa gcgacgataa gaaacttcgg gatccaaaac
     1081 aacggactct ttatgcttaa actgattgtt cttgccccgc tcgtgggctc gattctcctc
     1141 gcctttatcg gaaaacggct gtcggaacgg ctggttggtt tgctggcctg ctcaaccgtt
     1201 ggtatctctg ccatcacggc cttttacacc tttttcaccg cattgctgcc ggccaaagaa
     1261 ggcgctgacg gcattcgtcg ggtgaccgaa cacgtggcga cctggatgca ggtgggaacg
     1321 ttcaaagccg atttcggcct ggcgttggac ccgctctctg gcattatggt gctgtttgtg
     1381 acgggtgtgg gctttctgat tcatgtgttt gccaccggct atatgcatgg cgacaagagc
     1441 ttccatcgct ttttctgcta cctcaacctg tttatgtttg cgatgttgac gctcatcctg
     1501 gcagacaact tcttgttgat gtttgttggg tgggaaggcg tcggtgtgtg ctcatacctg
     1561 ttgatcggct actactttct gaagcaagag gctggcgacg ccgcgaagaa agcctttatt
     1621 ttcaaccgaa tcggtgactt tggcgtggtg ctgggcgtgc tgctgatttt taacaacttc
     1681 ggcacgatca actttgccga agtgatggaa aaagcggccc agttaccaac cgaacccttt
     1741 ggaatggcag ccgtgatggc tgggggattg accacgattg cgttgctcct ctttattggt
     1801 gcgaccggga agagcgccca gattccgctc tttgtctggc tgccggacgc gatggctggt
     1861 ccaacgcccg tttccgcact gatccacgct gcaacgatgg tcaccgctgg ggtgtacatg
     1921 gtggcacgct gcaacgcaat ttacagcaaa gctccaacgg ccttgctggt ggtggcggtg
     1981 attggcgggt tgacggcact gtttgccgcg accatcgggc ttggacagtg ggatatcaaa
     2041 aaagttctgg cgtattcgac cgtttcgcag cttggctata tgttcctggc gtgcggagtt
     2101 ggggcctaca cagcggcgat tttccacgtg atgacccacg cgtttttcaa agcgctgctc
     2161 ttcctcggtt ccggaagcgt gatccacgga atgcaccacg aacaggatat gcgctacatg
     2221 ggcggcctca agaaatacat gcccgtgacg cgctggacga tgatctgcgg ctggattgcg
     2281 attgcgggga ttccaccgtt tgccggtttc ttcagtaagg acgaaatctt gtggaagacc
     2341 tttagtacca ctgtgttacc aggagatgcc gggaagattc tgtggggact cggggtggtg
     2401 acggcgctgt tgaccgcgat ttacatgaca cgattgatgg tggtgacctt tgaaggcggc
     2461 gaacgctata aggaaagcca cggtcacgac gaccacg
//
LOCUS       DAOQDO010000823         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36850_length_2496_cov_3.714871, whole genome shotgun sequence.
ACCESSION   DAOQDO010000823 DAOQDO010000000
VERSION     DAOQDO010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36850_length_2496_cov_3.714871"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PKZ53_13225"
     CDS             <1..621
                     /locus_tag="PKZ53_13225"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ41448.1"
                     /translation="VVYDGLEHFQSAQAQGKGVLVLTGHFGSWELSAFSHALNGYPMN
                     ILVRRIDNPLIEALVNRYRTCSGNHTIDKNGSARAILTALRNRETVGVLSDLNMVRNQ
                     GVFCDFFGLSACSTPALATLALRTGAPVVPGFLIWEAAQKRYRLQFDPAVELIETGDS
                     KHDIEENTARFMKIIEHKVRAYPEQWLWIHRRWKTRPEGDPGLYQG"
     assembly_gap    681..780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1055..1573
                     /gene="nrdR"
                     /locus_tag="PKZ53_13230"
     CDS             1055..1573
                     /gene="nrdR"
                     /locus_tag="PKZ53_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNJ41449.1"
                     /translation="MKCPFCGHLEDKVVDSRESREGDVIRRRRECLKCERRFTSYERI
                     DEIPYMVVKKDGRREPFDRQKVLAGLLRACEKRPISMAKLEGIVVAVETYVQESRDRE
                     RSTEKIGEMIMRKLKELDKVAYVRFASVYLEFKDVTEFMAELKSLMKNPTSLEPPPPP
                     AKSSRKTKKSES"
     gene            1819..2412
                     /locus_tag="PKZ53_13235"
     CDS             1819..2412
                     /locus_tag="PKZ53_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41450.1"
                     /translation="MPLLIYKDNQELGPFDEADIADRLRRGELSPNDLGRYSQSSQWM
                     PLSTFFPPDPMAQPGSSGWSEAATQQIPYPSSSPYSTNPTGSSPYMPPPAPNLVYPSG
                     PMPVGSTGSPTRLPQIGMVLGIMTASLMVVGLVPCLGWVNWLTLLVGLVSLVICLIPL
                     ATEKDPQARNKAMIGILLTGGALVLGMIRLFFGGGCF"
BASE COUNT          564 a          616 c          608 g          608 t
ORIGIN      
        1 gttgtgtatg atggcctgga gcattttcaa tcggctcagg cacaaggaaa aggagttctg
       61 gttttaaccg gtcactttgg aagctgggaa ctcagtgcgt tttcacatgc cctgaatggg
      121 tatccgatga acattctggt tcgccggatt gacaatccgc tgattgaagc gctggtcaat
      181 cggtaccgga cctgttccgg caaccacacc attgacaaaa atgggtctgc ccgcgcgatt
      241 ttgactgctc tgcgcaaccg ggaaaccgtt ggggttctgt ctgatttaaa tatggtccgg
      301 aatcagggcg tcttttgtga tttttttggc ctttctgcct gctcaactcc ggcactggca
      361 acacttgcat tgcggactgg ggcaccagtt gttccagggt ttttgatctg ggaggccgct
      421 caaaaacgat accgcttgca gtttgacccg gctgtcgagc tgatcgaaac tggtgattcg
      481 aaacacgata tcgaagaaaa tactgcccgg tttatgaaaa tcattgagca taaagtgcgg
      541 gcttaccctg agcaatggct atggattcat cgccgatgga aaacccgtcc tgaaggcgac
      601 cccggtttgt accagggctg aagacatcgg gctgaagaag tcgggctgaa gacgtcgggc
      661 tgaaggcatt taaccctttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 tcttcagccc caagcccaat gtcttcagcc ctgagccctg gtttcttcag ccccaagccc
      841 aatgtcttca gccctgagcc ctggtttctt cagccccatg tcttcaacct ccaaatcaaa
      901 actgttcaaa ctgctcttcc gcgattacac ttccctcacc aggtctgtgt tccttccacc
      961 tcaccacgat tgatcgaaat cacccggcag acagcagtct tcctgccatt cctttgtcag
     1021 cacaggctgc tgaacagaaa gggattcctg tagtatgaag tgcccatttt gcggacacct
     1081 ggaggataaa gttgtcgatt cccgcgaaag tcgtgaaggc gatgtgattc gtcggcgccg
     1141 tgaatgcttg aagtgtgagc ggcggtttac gtcgtatgag cggattgatg aaattcccta
     1201 catggtcgtc aaaaaagatg gtcgccgtga accttttgat cgccagaagg tgctggctgg
     1261 cttgctccgc gcctgtgaaa aacggccaat cagtatggcc aaactggaag gcatcgtggt
     1321 ggccgtcgaa acctatgttc aggaatcacg tgaccgtgag cgatcaaccg aaaaaatcgg
     1381 ggaaatgatt atgcgcaaac tcaaggaact cgacaaagtg gcctatgtcc gctttgcgtc
     1441 ggtttacctt gaattcaaag atgtcacaga gtttatggcc gaactcaaat ccttgatgaa
     1501 aaacccgacc tcgctcgaac ccccaccccc accagccaaa tcaagtcgga agaccaaaaa
     1561 gagcgaatcg tgaatcaaac gtgagtcgcg agtagtaaaa agcgatttga cacccgaagc
     1621 cgcgccttgt gaagtatgaa ctgtgaaaaa caggttggat cccaggcaat tttgcctgat
     1681 cagattgaat ttgggccagt aaccttgaat actgagtcat aacctgatct tcacaaaaaa
     1741 cagttatttt gctactcgct attcgctacc cagggttcgc tttagttcgc ttgtatctcc
     1801 aaaggagctt caaccccaat gcctctcctt atttataaag ataatcagga acttggccct
     1861 tttgatgaag ctgatattgc cgacaggttg cggcgaggag agctttctcc aaatgatctg
     1921 gggcggtatt cccaatcatc acagtggatg ccgttgagta cctttttccc gccagaccca
     1981 atggcacaac ctgggtcatc tggatggagc gaagctgcca cccagcaaat tccgtatcca
     2041 tcatcgtctc cctactcgac caaccccacc gggtcgtcac cctacatgcc gccaccggcg
     2101 cccaaccttg tctatccttc tggaccaatg ccagttggtt caactggtag cccaaccagg
     2161 ctccctcaga ttggaatggt gcttggaatt atgaccgctt cactgatggt ggttgggttg
     2221 gtgccatgtc tggggtgggt caactggctt acacttttag ttggtctggt ctcactggtg
     2281 atttgtttaa ttccgctggc cactgaaaaa gacccccagg cccgcaacaa agccatgatc
     2341 ggcattttat tgactggcgg agcactggtt ctcggtatga ttcgcctgtt tttcggcggc
     2401 ggctgttttt gaatgaagaa tgaagaatga agaatgaaga aatgggttcc gggttccggg
     2461 ttccgggttc cgggttccgg gttccgggtt ccgggt
//
LOCUS       DAOQDO010000824         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36894_length_2495_cov_2.225820, whole genome shotgun sequence.
ACCESSION   DAOQDO010000824 DAOQDO010000000
VERSION     DAOQDO010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36894_length_2495_cov_2.225820"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKZ53_13240"
     CDS             complement(<1..489)
                     /locus_tag="PKZ53_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41451.1"
                     /translation="MSYHTLQRLVVRMLFDDQFVSQVYANPDQALANLELTPIEQQQL
                     IQVDQRAWGYDPLRRKRTLRTLVEEFKASTTLALAETRSLALLDQFFSSSFFHTSVQN
                     RGSMGLAFSQFLSDLTKQGTLTSPQLPDVLKLETLMAGCRRELDAAGGPVQNALPGSI
                     DEH"
     gene            complement(486..1382)
                     /locus_tag="PKZ53_13245"
     CDS             complement(486..1382)
                     /locus_tag="PKZ53_13245"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNJ41452.1"
                     /translation="MASSFFQTHLHTLPRLGVGISGEYGSASKGIDACAMKELYPELL
                     HFFEYGCDIDRGLDEHVRHWAGSGLPTTYHFLDINLEEKIDSDRHWLTRTQMLASEIN
                     AAWLCGDAGRWHFGPRERGHQMLMPPILCEESATQTAESIQWIQSETGRLVLPENPPA
                     VVYLGDMHILDYFGKVADQADCGILLDCAHLSIFQHSRGHSPLTGFDGFPFERVIEIH
                     VAGGTPVESNGFGYIDDTHSPEPIPETWEILEHVVKHATNLRAIVYECEHNVLEACLD
                     NFARLNALFPASTQISAEVAPL"
     gene            complement(1667..2353)
                     /locus_tag="PKZ53_13250"
     CDS             complement(1667..2353)
                     /locus_tag="PKZ53_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ41453.1"
                     /translation="MTTIGILGTGRMGVRLALLFAQTGNQVLLASRDVARAQRITDSL
                     DLPNLRPATYSEALTAPVIVPSIFVRDGLLDELVTWKTQLAGKLLIDITNPFNDDYSD
                     FILPWDTSSAEQIQHRLPETKVVGAFKNVWWEVFDQPQFGTEISDVYVVSDHPEAKQV
                     FFKLVEGSSFRYVDAGRLANARTVERMTLLSGEMGQRLGIKPRLNYKLLGTPWEVGRG
                     NPELDALIAR"
BASE COUNT          610 a          634 c          662 g          589 t
ORIGIN      
        1 gtgttcgtca attgaaccgg gcaatgcgtt ctgaactggc ccaccagccg cgtcaagttc
       61 gcggcgacac ccggccatca gtgtttcaag cttgagcacg tctggcaact ggggcgacgt
      121 caatgttcct tgtttggtca aatcggacag aaattgtgag aaggcgaggc ccattgaacc
      181 ccggttctga actgaagtgt gaaaaaaaga actcgaaaaa aactggtcaa gcaacgccag
      241 tgaccgtgtc tcggccagag ccagtgtggt cgaggcttta aactcctcaa ccagcgtgcg
      301 cagggtgcgc tttcgtcgga gcggatcata tccccaggcc cgttgatcaa cctggatgag
      361 ttgctgctgt tcaatcgggg tcaattcaag attggccagt gcctgatccg ggttggcata
      421 cacctgactg acaaactggt cgtcaaagag catgcgcacc accagccgtt gtaacgtgtg
      481 atagctcata gcggtgcaac ctccgctgaa atctgggttg acgccggaaa cagagcattc
      541 aggcgggcaa agttatcaag gcaggcttcg agcacgttgt gttcgcattc atacacaatg
      601 gcgcgtaaat tagtggcgtg tttgacgacg tgttccagga tttcccaggt ttctggaatt
      661 ggttctggtg agtgggtatc gtcaatgtag ccaaacccgt tggattcaac cggagtgcct
      721 ccggcaacgt ggatttcaat gacccgttca aatgggaatc catcgaatcc ggtcagcggt
      781 gaatggcccc ggctgtgttg aaagattgag aggtgggcac aatcaagtaa aattccacaa
      841 tcggcctgat ccgccacctt cccgaagtaa tccagaatgt gcatatcgcc cagatatacc
      901 accgctggcg ggttttccgg caggaccagc cttccggttt cagattgaat ccactggatg
      961 gactctgccg tctgagtggc tgattcttcg cacagaattg gcggcatcag catctggtgg
     1021 ccgcgttcac gggggccaaa atgccagcgc ccggcgtctc cgcacaacca ggcggcgttg
     1081 atttcggaag ccagcatctg agttcgagtc aaccagtggc ggtccgagtc aattttttct
     1141 tccaggttaa tatcgagaaa atggtaggtg gtcggaagac ctgacccggc ccagtgtcgg
     1201 acgtgttcat ccagcccacg gtcaatgtcg cagccatact caaagaaatg aaggagttcc
     1261 ggatacagct ctttcattgc acaggcgtca attccttttg aagccgagcc atattcaccc
     1321 gaaataccaa cgcccagccg gggaagagtg tggagatgtg tttgaaaaaa tgaagatgcc
     1381 ataaaacagg gttcagggtt tgaaaccagg gttcagggtt tgaaaccagg gttcagggtt
     1441 cagggttcag ggtttgaaac cagggttcag ggctcaggga agaaaaggat tttagcccgg
     1501 aacaggcatc gtgcaatagc cgtggtggga aaatccgggt tccgcgcttc cgcacttccg
     1561 ggctccgcac ttccgggttt tcgaagttat gtcctttatg tcctttatgt ccctttggtc
     1621 ctttgaaccc tcaaccctca accctcaacc ctcaactctg ttttcctcag cgtgcaatca
     1681 gggcatcaag ctcgggattc ccgcgaccaa cttcccacgg tgttccaagg agtttgtaat
     1741 tgaggcgtgg tttgattccc aatcgctggc ccatttcgcc gcttaacagc gtcatgcgtt
     1801 cgacagttcg ggcattggcg agccgccccg catcaacata ccgaaacgaa gagccttcga
     1861 ccagtttgaa aaacacctgt ttggcttcgg ggtggtcact gacaacatag acatcactga
     1921 tttcggttcc aaactgcggc tggtcaaaaa cttcccacca gacattttta aatgcaccaa
     1981 ccactttggt ttctgggagg cggtgctgga tttgttcggc gctgctggtg tcccagggaa
     2041 gaataaaatc tgaatagtca tcattaaacg ggttggtgat atcaatcaga agctttccgg
     2101 caagctgtgt tttccaggtt accagttcgt ccagtaatcc gtcgcgaaca aagatagacg
     2161 gcacaatcac cggagccgtc agtgcttccg aataggttgc cgggcgcaga tttgggaggt
     2221 caaggctgtc ggtaattcgt tgagcccgcg ccacatcacg ggaagccagt aaaacctgat
     2281 ttccagtttg cgcaaagaga agagccagtc tgacgcccat ccgtccagtt ccaagaattc
     2341 caattgtggt cataagttgc tcctttggct aaaaactacc tatcagaagg tagagaatca
     2401 aaagtgaaaa atacctacct tctacaaggt aggtttgttc caaaaaaatt aaatttcgtc
     2461 gagtaagagt ttcagcaaac catcccggac atccg
//
LOCUS       DAOQDO010000825         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36971_length_2492_cov_3.202708, whole genome shotgun sequence.
ACCESSION   DAOQDO010000825 DAOQDO010000000
VERSION     DAOQDO010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36971_length_2492_cov_3.202708"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..>2492
                     /locus_tag="PKZ53_13255"
     CDS             41..>2492
                     /locus_tag="PKZ53_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41454.1"
                     /translation="MPFQNPSESPVGEIFGLSEDNSQTLWMTTATGLYSLIGDLIFKA
                     EPGAVNVPLTRCVTDALGNIWCVPTGKGVVVYDPLRREAAELLATDQVLTLAVDREGS
                     LWFGTNEGAIYHDVFSFVGFDTSRGLTDSDVYAIAPDIARKHLWVGTSRGVSEFDGVQ
                     FRPLSQLPPDIMVRSLMVAPDGALWIAASTELFRWDGVNLLKTEATVFPKLELQDIVI
                     DTKRPLVWVATTNAVIPINPTTLKPEGEPVRIDGKVRFLTQASSGLLWIATDRGVYRY
                     DPTKAELVVIGTDQGLKSLDVRWILEQPTKGQFWFATGHSVESYDGNRFDLEPISGAT
                     KGADVRCLFLDRDGFLWVGLGDGNIRKFLPQIPGLTKTYKRDDYDLTGTQIRTITQDE
                     AGIVWFATDQGVTRHVPNRLSPPIDIKLEVDGAEINQPSPVYVSSGQHRLKFQYVGIS
                     MMSDVAYLYRLVGDKTGNDEWKLIDADRPNKTESPVMDLPPGEYTFEVRALNRDLYGW
                     NALPTQLTVRIDRPIYTKPWFLALGVTLLASGGTGVFLIRRHQRREYILPPHLKTFRA
                     IEPNPYIVGNPIRQPQMFFGREDDFNYVRKKLEGAAQGLVIVFCGERRTGKSSILYQI
                     QNGRLGTRFVPVFIDMQEMIIESDGEFFQRMARLIAESVHRYLGPESTLEFPFHSQSG
                     TPRYDSKRYPFTQVGTNPFDLFREFINEVLRMIGDRRLILLVDEYELLETKVDNKKLN
                     REIFAFLAGLIDSHDQLSFIFTGSKKIEQRDRRYWREMLRRSLFRKVGYLSQRDTFRL
                     TTEPVRDQVVYGRGVVERI"
BASE COUNT          578 a          607 c          681 g          626 t
ORIGIN      
        1 tgatcggggc ttgttccgtt ttaatggcca atggtctcaa atgccgttcc agaatccatc
       61 tgagagcccg gttggtgaaa tttttggctt gagtgaggac aattcacaaa cgctgtggat
      121 gaccacggcc acggggctgt actcgctgat tggggatctt atttttaaag ctgaaccggg
      181 tgccgtcaat gtccccctca cccgatgtgt gactgatgcc ctgggaaaca tctggtgtgt
      241 accaactgga aaaggcgtcg tcgtttatga cccactccgg cgagaagcag ccgaactgct
      301 tgcgacggat caggttttga ccctggcggt ggaccgggaa ggcagcttat ggtttggtac
      361 caatgaaggg gcaatttatc acgatgtctt tagttttgtt gggtttgaca ccagtcgtgg
      421 tctaacggac agtgatgtgt atgccattgc cccagatatc gcccgcaaac acctctgggt
      481 tggaacctcg cgcggcgttt ccgaatttga cggggtccag tttcgtccgc tcagccagtt
      541 accgcccgat atcatggttc gcagcctgat ggtggcgccg gatggggcac tctggattgc
      601 agcttccacc gaattatttc ggtgggatgg ggtcaatctt ctcaaaactg aagcgacggt
      661 cttccccaaa cttgagctgc aagacattgt gattgacacc aagcgaccgc tggtgtgggt
      721 tgccaccacc aatgcggtga ttccaatcaa cccgacgacg ctcaaacccg aaggtgaacc
      781 ggtccgaatt gatggaaaag tcagatttct gacccaggcc tcgtctggat tgttgtggat
      841 tgcgacggat cggggggtgt atcggtatga tccgaccaaa gccgaactgg tcgtcattgg
      901 caccgaccag ggcctgaaga gtctcgatgt acgctggatt ttagagcaac ccaccaaagg
      961 tcagttctgg tttgccactg gacatagcgt tgaaagttat gacggcaatc gctttgacct
     1021 ggaaccgatc agtggtgcca ccaaaggcgc agatgttcga tgtttgtttt tggatcggga
     1081 tggatttttg tgggttgggc tcggagatgg aaatatccgc aagttcctgc cgcaaatccc
     1141 tggactgacc aaaacctaca aacgcgacga ttatgatctg actggcaccc agattcgcac
     1201 gattactcag gatgaagcgg gtattgtctg gtttgccaca gaccagggag taactcgtca
     1261 cgtccccaac cggctatcac ctccgattga tattaaactt gaagtggatg gtgctgaaat
     1321 caaccagccc tcgccagtgt atgtgagttc gggtcagcac cggcttaaat ttcaatatgt
     1381 tggcatcagc atgatgagtg acgtggcgta tctctatcgg ctggttggcg ataagaccgg
     1441 aaatgatgaa tggaaactga ttgatgcgga tcggccaaat aaaactgagt caccagtgat
     1501 ggatttgccg ccaggggaat atacctttga agttcgggcc ttaaatcggg atctctatgg
     1561 ctggaatgcc ctgccaaccc agttgaccgt acgcattgat cgtccaatct ataccaaacc
     1621 ctggtttctc gccttagggg ttaccttgct ggccagcggg ggaacgggcg tctttctgat
     1681 tcgacgccac cagcgccgtg aatatatcct ccctccacat ctcaaaacat tccgcgccat
     1741 cgagcccaac ccatacattg tgggaaatcc gattcgccag ccacaaatgt tttttggacg
     1801 cgaagatgat ttcaattatg tccgcaaaaa actggaagga gccgcccagg gactggtgat
     1861 tgtgttttgt ggcgaacgcc ggaccggaaa aagctcgatt ttgtatcaaa tccagaatgg
     1921 ccggttggga acgcggtttg tgccagtgtt tattgatatg caggaaatga tcatcgaaag
     1981 cgacggtgag ttttttcagc gcatggcccg cctgattgcg gaatcagttc atcgctacct
     2041 gggaccggaa agcacccttg aattcccctt tcattcccaa tccgggacgc ctcgctatga
     2101 ctccaagcgg tatccattta cccaggttgg caccaatccg tttgatttgt ttcgggaatt
     2161 catcaatgaa gtgttgcgca tgattggtga ccgacgattg atcctgctgg tggatgaata
     2221 tgaattgctc gaaaccaaag ttgacaataa gaaactcaac cgtgaaattt ttgcctttct
     2281 ggctggtctg attgatagcc acgatcaact ttcgttcatt tttaccgggt cgaagaaaat
     2341 cgaacagcgg gaccggcgct actggcgtga aatgttacga cggtcgctct tccgcaaagt
     2401 cggatacctg agccagcgcg atacgttccg gttgacgaca gagccagttc gcgaccaggt
     2461 ggtgtatgga cgtggggtcg tcgagcgaat tt
//
LOCUS       DAOQDO010000826         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36973_length_2492_cov_3.104637, whole genome shotgun sequence.
ACCESSION   DAOQDO010000826 DAOQDO010000000
VERSION     DAOQDO010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36973_length_2492_cov_3.104637"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PKZ53_13260"
     CDS             <1..867
                     /locus_tag="PKZ53_13260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNJ41455.1"
                     /translation="RVVQVPKSIDNDLSLPVSVETLGFETARHVGVEMVKNLMVDART
                     TGRWYFGVTMGRPTGHLTLGIGKAASAACTIIPEEFEQETISLSDICDIVEGVIIKRR
                     AMGRPDGVILLSEALNERFHPDEVAELQDVDRDAQGNIRVTEIDLGRKVKNEVQLRFE
                     RRKIKVTIVDKTIGYELRCAPPVPYDAEYARDLGYFAVNYLLKGGSSSMITIQGGEFK
                     PIPFSEILERSGGHGRRRYVDITADNYKVARDYMLRIGPRDFANPDWVGQLAAAGGLS
                     VDDFRQRFEKFA"
     gene            complement(875..1771)
                     /locus_tag="PKZ53_13265"
     CDS             complement(875..1771)
                     /locus_tag="PKZ53_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41456.1"
                     /translation="MPISFRISTWLVGLVLLCLLTSCAENKEKQFETALKAAEKGRNV
                     RQLYTLLKTHPEFQARTRQVLKTYYTLAQQKLGQSPAEAKIKTALSQILTDLEKQQTT
                     VVRVSFSRDDYRETQRKADADARERVNAQNQADTSGTSPYKYHQIQIELQMLADAETF
                     FCQTVSDELQKFFESNVIEFERTTDTVRPVNSEVKLHYQIKWTGDLYKFDSTPPDTSF
                     HVYPGLEVENKIDLIAAGNQVTFQSKEKSSSTFQVETDSVKAAQSPPDPSKVYLTMFS
                     NAFIESGKHYANFLSGAETKMK"
     gene            1880..2461
                     /locus_tag="PKZ53_13270"
     CDS             1880..2461
                     /locus_tag="PKZ53_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ41457.1"
                     /translation="MSLQLPGDLNGFIAALETISRDARQTFGDLTHDQINWKPTAQQW
                     SIGQCFDHLITTNRTYFPTYEAILRGDKKSTIWEKLPVLPGFWGSWLIKTLLPDSGKK
                     LKAPPAFAPTQSTVEAGIIRRFQDEQAQLINFLKQMAHIDLKKTIITSPAAAIVTYSL
                     FDTCTITVVHEHRHFQQAKRVMDVAGFPLKNQG"
BASE COUNT          583 a          653 c          643 g          613 t
ORIGIN      
        1 cgggtcgttc aggtaccaaa aagcattgat aacgatttat cactgccggt ttcggttgaa
       61 acgctcgggt ttgagaccgc tcgccacgtc ggggttgaaa tggtcaaaaa cctgatggtg
      121 gacgcccgaa ccactggccg ctggtatttt ggcgtgacca tgggccgacc gaccggacat
      181 ttgacgctgg ggattggaaa ggcagcaagt gccgcctgta ccatcattcc cgaagaattt
      241 gaacaagaaa ccatttcgct cagtgacatc tgcgacattg tcgaaggcgt gatcatcaag
      301 cggcgggcaa tgggccgacc tgacggcgtg attttgctgt cagaagccct caacgaacgc
      361 tttcaccccg acgaagttgc cgaactccag gacgtagacc gtgatgctca gggcaacatt
      421 cgggtcaccg aaattgacct gggccgcaaa gtcaaaaacg aggtccagct ccggtttgaa
      481 cgccgcaaaa tcaaagtaac aattgtggat aaaacaattg gctacgagtt gcggtgtgcg
      541 ccgcctgtac cgtatgacgc cgaatatgcc cgtgatctgg gatatttcgc cgtcaattac
      601 ctgctcaaag gtggttccag ctcgatgatt accatccagg gcggggaatt taaaccaatt
      661 ccgttctcag aaatcctgga acggtccggc ggtcatggtc gtcgccgcta cgttgacatc
      721 acggctgaca attacaaagt ggcccgtgat tatatgcttc gcatcgggcc acgtgacttt
      781 gccaatccag attgggttgg gcaactggcg gcggctgggg gactgtcagt tgatgacttc
      841 cgtcagcggt ttgaaaagtt tgcctgaaat caattcactt catctttgtc tcagcgccgc
      901 tcaggaagtt cgcatagtgc ttgccggatt caataaaagc gttgctaaac atcgtcaaat
      961 acacttttga cggatcaggt ggtgattggg cggctttcac tgagtcggtt tcgacctgaa
     1021 acgtcgagct gcttttctcc ttcgattgaa atgtgacctg gttgccagcg gcgatcaggt
     1081 caattttgtt ttcgacttcc agaccagggt agacgtgaaa ggacgtatcc ggcggggtgc
     1141 tgtcaaattt gtacagatct ccggtccact tgatttgata gtggagcttg acctcggaat
     1201 tgaccggtcg cacggtgtcg gtggtgcgtt caaattcgat cacattgctt tcaaagaact
     1261 tctggagttc atcgctgacg gtctggcaaa agaaggtctc ggcgtctgcc agcatctgca
     1321 attcaatttg aatctggtgg tacttgtacg gagacgttcc actggtatca gcctgattct
     1381 gggcattgac ccgttcgcga gcatcggcat cggctttgcg ctgggtttcg cgatagtcat
     1441 ctcttgaaaa actcacgcgc accaccgtcg tctgctgctt ttccaggtcc gtcagaatct
     1501 ggctcaaggc cgttttgatt ttggcttcgg cgggggattg ccccagcttc tgttgtgcca
     1561 gcgtgtaata ggttttcaac acctgtctgg ttcgagcctg aaactcggga tgtgttttga
     1621 gcaacgtgta aagctgtcgg acatttcgac ctttttcagc cgctttgagc gccgtttcaa
     1681 actgcttttc tttattttca gcgcagcttg tgagcaggca caggagaacc aatccgacca
     1741 gccaggttga gatgcgaaac gaaatgggca tttgatatgg atagaatcgt caaaattgga
     1801 taagcttgcc gcaagcttgt ccaatttcca atcaatcagt caagaccggc gccatcttct
     1861 tttccaaatc ggagtcccta tgagtcttca actccctggt gacctcaacg gtttcattgc
     1921 cgcacttgaa accatcagcc gcgatgcccg gcagacgttt ggcgacttaa cccacgacca
     1981 gatcaactgg aaaccaactg cgcaacagtg gagcatcggc caatgttttg atcatctcat
     2041 caccaccaat cgcacctact ttccgaccta tgaagcgatt cttcgtggcg acaaaaagag
     2101 caccatctgg gaaaaactcc ccgtgcttcc tggtttttgg ggaagctggc tgattaaaac
     2161 actcctgccc gattcaggaa aaaaactcaa agccccgccg gcatttgccc caacccaaag
     2221 taccgttgag gcgggcatca tccgccggtt tcaggatgag caggcccagc tcatcaactt
     2281 cctgaaacaa atggctcaca ttgacctcaa gaaaaccatc atcacctcgc cggcggcggc
     2341 catcgtgacc tacagtttgt ttgatacctg caccatcact gtggttcacg aacaccgtca
     2401 tttccagcaa gccaaacgcg tgatggacgt cgccggattc cccttgaaaa accagggctg
     2461 aagactcgca agctcggggc tgaagaagtt gc
//
LOCUS       DAOQDO010000827         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_36978_length_2492_cov_2.740254, whole genome shotgun sequence.
ACCESSION   DAOQDO010000827 DAOQDO010000000
VERSION     DAOQDO010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36978_length_2492_cov_2.740254"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PKZ53_13275"
     CDS             <1..1183
                     /locus_tag="PKZ53_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41458.1"
                     /translation="LDLLYSLTPYVAVAIENSLLYQSELERTQELALLKEFNENIIES
                     INVGILTVDPQGLITNCNSAFEELFSVSREQAEGQSVEQLFDADLVQTMRDVTGSVSW
                     ALSDVRNIYRYRATTRTEEPLVLNINLSPLKTKDEAVTGALIAFEDITSRVRLEEQLR
                     ENDKLSSIGLLAAGVAHEVNTPLTGISSYTQMLLTQTPSSDVKHSILEKIHRQTIRAS
                     DIVNNLLNFSRTGNASFSQLSIAKLLDDTLQLLELQIRNTRIEIVREYSHTLPPVLGQ
                     AGKLQQVFLNLILNARDAMPDGGTLRISASVHEDSLVVDFADTGEGIPSEYIAKIYDP
                     FFTTKEVGRGTGLGLAVSYGIIQEHSGRIFVESEPGHGTCFRVKLPCQVHHASFQAVG
                     D"
     gene            1448..2434
                     /locus_tag="PKZ53_13280"
     CDS             1448..2434
                     /locus_tag="PKZ53_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ41459.1"
                     /translation="MKKSPLLKLKQKLREAHLVAKGLLSTSHPILVHIIPMRRCNLAC
                     TYCNEYDKTSDPVPLDVMYRRIDKLAELGTTIVTESGGEPMMHPNIYDIIARVRQHGM
                     MSGIITNGYYLSPERIKLLNEAGLEYLQISIDNVTPDEVSQKSLKVLDKKLQNLAQYA
                     QFEVNINSVIGGGIKNPQDAITVGRRAAELGFSVSVGIIHDGHGHLKPLTPDEAEVYR
                     QLKKIGKSGHSQITQFQDALAEGRPNQWKCRAGARYLYICEDGLVHYCSQQRGYPGIP
                     LENYTRDDIIREYNTPKPCADYCTIACVHKVSKMDFWRDPQYHQPTMVETGK"
BASE COUNT          643 a          708 c          561 g          580 t
ORIGIN      
        1 ccttgattta ctctactcgc tgacccccta tgtggccgtc gccattgaaa acagcctgct
       61 ctatcaatcc gagttggaac gaacccagga actggcactc ctcaaggaat ttaacgaaaa
      121 tatcatcgaa agcatcaatg tcggcattct caccgttgac ccccagggcc tgatcaccaa
      181 ttgtaacagc gcctttgaag aactcttctc ggtcagtcgc gaacaggccg agggccaatc
      241 cgtcgaacaa ctctttgatg ctgatctggt tcaaaccatg cgcgatgtga cgggaagcgt
      301 gagctgggcg ctctctgatg tgcgcaatat ctaccggtat cgggccacca cccgcactga
      361 ggaaccactg gttctcaata tcaacctctc ccccctgaag accaaagatg aagccgtcac
      421 tggcgccttg attgccttcg aagacattac cagccgggtc cgcctcgaag aacaactccg
      481 tgaaaacgac aaattatcgt ccattggctt gctggctgcc ggtgtcgccc acgaggtcaa
      541 tacaccattg accggtatct ccagctatac ccagatgctg ctgacccaaa ccccatcctc
      601 cgatgtcaag cactccattc tggaaaaaat ccaccgtcag accattcgtg cctcagatat
      661 cgtcaataac ctcctgaatt tttcgcgaac cggaaacgcc agcttttcac aactcagcat
      721 cgccaaactg ctcgacgata ccttgcagtt gcttgaactt caaattcgca atacccgcat
      781 tgaaatcgtt cgtgagtata gccataccct gccaccggtc ctcggtcagg ccggcaagct
      841 ccagcaggtc tttctcaacc tgattctcaa tgcccgcgac gccatgcccg acggaggcac
      901 cctgcgtatc tcagcttctg tccacgaaga ctcactggtt gttgactttg ccgataccgg
      961 tgaaggaatc ccctctgaat acatcgccaa aatttatgac cccttcttca ccaccaaaga
     1021 agtagggcgt gggacaggcc tcggcctggc cgtcagctat ggcatcattc aggaacattc
     1081 tggccggatc tttgtggaga gtgaacccgg ccatggcacc tgttttcggg tcaaactccc
     1141 ctgccaggta caccacgcct catttcaggc agttggagat taggaggact ggagctgggg
     1201 gctgaaatac tcgggctgaa agtctcgcaa gctcggggct caagaaaacg gggagacaga
     1261 gtccgtaacc cgctgtcttc cgccccagat cttaaggcgt ttcgattaga cttttcagaa
     1321 gggttggttt ataacttgcg gcttgtttcg ctcgatccat cgatctcgtt tctcgataga
     1381 ccaactccga caatccaaat tttcctttcg tggaatgggg cttccccccc ggttcccttg
     1441 ctggtgcatg aaaaaaagtc cattactcaa actcaaacaa aaattgcggg aagcccacct
     1501 ggtagccaaa gggcttcttt caacctctca tcccattctg gttcatatca tcccaatgcg
     1561 ccggtgtaat ctggcctgca cctattgcaa tgaatacgat aagacttctg atcctgtccc
     1621 attggatgtg atgtaccgcc gaatcgacaa actggccgaa ctgggcacca ccattgtcac
     1681 cgaaagtggg ggtgaaccca tgatgcatcc caatatctac gacatcattg cccgggttcg
     1741 tcaacacgga atgatgtctg gcattattac caatgggtat tatctctcac ctgagcgcat
     1801 caagttgctc aatgaagctg gcctggaata ccttcaaatc agtatcgata atgtcactcc
     1861 ggacgaagtt tcccagaaaa gcctgaaggt tttggataaa aaattacaaa acctcgctca
     1921 gtacgctcaa tttgaggtca atatcaattc ggtcattggc ggtggaatca aaaatccgca
     1981 ggacgccatt accgttggtc gtcgagccgc ggaactcggg ttttcagtct cggttggcat
     2041 catccatgat ggccacggtc atctcaagcc actcaccccc gacgaagccg aagtgtaccg
     2101 gcaactgaaa aaaatcggaa agtccggcca ttctcaaatt actcaatttc aggatgccct
     2161 ggccgaagga cgccccaacc agtggaaatg tcgagctggc gcccggtacc tctacatctg
     2221 tgaagatggg ctggttcact attgctccca acaacgaggc taccctggaa tcccccttga
     2281 aaattacacc cgtgacgata ttatccgcga gtacaatacc ccgaaaccct gtgccgatta
     2341 ctgcacgatt gcctgtgttc acaaagtctc gaaaatggat ttctggcgtg accctcaata
     2401 tcatcaacca accatggttg aaaccggaaa atagctcttt gaatgaagaa ttgagaatga
     2461 agaatgaaga atgaagaatg aagaatgaag aa
//
LOCUS       DAOQDO010000828         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37049_length_2490_cov_2.166324, whole genome shotgun sequence.
ACCESSION   DAOQDO010000828 DAOQDO010000000
VERSION     DAOQDO010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37049_length_2490_cov_2.166324"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..719)
                     /locus_tag="PKZ53_13285"
     CDS             complement(84..719)
                     /locus_tag="PKZ53_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNJ41460.1"
                     /translation="MPVPEHMEFFIDPNRCIGCQSCVQACGECDTHKGHPMIHLEYVD
                     RALSVQTTPVVCMHCNTPTCAEVCPADAIKVTEDGVVQTARKPRCIACSNCVLACPFG
                     VPKMKTEFELMMKCDMCYDRTSVGKKPMCATVCPSQALFFGTREEIEQIRPRSVPNNR
                     FQFGHQVITTKVNMLFPKDNPIQHLDVTDAMNEKPVDQSFTLEMLMDAIGI"
     gene            complement(818..>2490)
                     /locus_tag="PKZ53_13290"
     CDS             complement(818..>2490)
                     /locus_tag="PKZ53_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNJ41461.1"
                     /translation="DNTRTPFGLQIWISGANVAECAPITTNYVWQAREHGAKIIVVDP
                     RITPIARTCDLFLPIKPGRDIALFNGILHLMIENDWLDHDFIRNSTVGFEAVAGHVKA
                     WTPKHTAEVTGIAEKSIRQAAEWWGTAQTSFLMHARGIEHHSHGVQNVLGAINMVLAS
                     GRIGRENCGYATITGQGNGQGGREHGQKCDQLPGGRDISNPEHRAYVAKVWGVSPDEL
                     PQAGVDAYEIFRKIDRGEIRGLLSLCFNPKVSLPDNAFVTKALEKLEFYVAIDFFLNE
                     TAHHADIVLPGSLHEEDEGIVTQIEGRVIKINKVIDPPGEARQDWRIIQDIARQMGAE
                     KGFTFASPKEMLDELRIASKGGVADYSGITYEKVERQHGVFWPCPSDDHPGTPRLFEP
                     GSWNPIAKGAGPFYFPDGKARFVVAPYTPPTEDVDTEYPIILTTGRVISHFLSGSQTR
                     RIGPLVDQYPEPRVEIHPRLAEKLGIANGDLTTVESRRGNCTLKAHVVTTIRPDTVFI
                     PYHWANRQSANQLTISAQDPISKIPEYKVCAVKVFKHRAEDTGLKTSG"
BASE COUNT          562 a          625 c          687 g          616 t
ORIGIN      
        1 tgaagaatga agaatgaaga atgaagaatg aagaatgaag aatgaagaat gaagtccgtc
       61 atcaagtcat ctggtcagtt ctgtcaaata ccaatcgcgt ccatcagcat ttcaagtgtg
      121 aagctctggt caaccggttt ttcattcatg gcatcggtca catccagatg ttgaattggg
      181 ttgtcttttg gaaacagcat attgactttg gtggtaatca cctgatgtcc aaactgaaag
      241 cggttattag gaactgaccg tggtcgaatc tgctcaattt cttcacgtgt cccgaaaaac
      301 agcgcctgac ttgggcaaac tgtggcgcac attggctttt tcccaaccga ggtgcggtca
      361 tagcacatat cgcacttcat catcagttca aattcagtct tcatcttggg gacgccaaat
      421 ggacaggcca gaacacagtt gctacaggca atacaccggg gtttgcgtgc cgtttgaacc
      481 acgccatctt cagtcacctt gatcgcatca gccggacaga cttcagcaca ggtcggcgtg
      541 ttgcagtgca tacacacgac cggcgtggtc tgaactgata gtgcccgatc aacatattca
      601 agatggatca tcgggtgtcc tttgtgggta tcacattcac cacacgcctg gacgcaagat
      661 tgacatccga tacagcgatt cggatcaatg aaaaattcca tgtgttcggg cactggcata
      721 ggggggcatc ctttgttttg aggcttgggg cttggggttg aagactcgca agctcggggc
      781 tgaagaaaac gggttcaata ccccgagccc aacttcttca gcccgacgtt ttcagcccgg
      841 tgtcttcagc ccggtgttta aacactttca ccgcacagac cttgtattcc gggattttgg
      901 aaattgggtc ctgggcagaa atagtgagct ggttggcact ctggcgattg gcccagtgat
      961 agggaataaa cactgtatca ggccgaatcg tggtcacgac gtgggctttg agggtgcaat
     1021 ttccacgacg tgattcgacg gtggttaaat cgccattggc gattcccagt ttttcggcca
     1081 gccgggggtg tatttcaact cgtggctccg ggtattgatc aaccagcggg ccaattcgtc
     1141 gggtttgcga gccagaaaga aaatgactga tcacccggcc tgtggtcaga atgattggat
     1201 attcggtatc aacgtcttcg gttggcggtg tgtagggggc gacgacaaac cgggcttttc
     1261 cgtcagggaa gtaaaatggc ccggcgcctt tggcaatcgg attccacgaa cccggttcaa
     1321 acaggcgcgg ggttccaggg tggtcatcgc tcggacaggg ccaaaatacc ccgtgctggc
     1381 gctctacttt ttcataggta atgcctgaat aatctgccac cccgcctttg gacgcaattc
     1441 gcagttcatc cagcatttct tttgggctgg caaaggtaaa gcctttttct gctcccatct
     1501 gacgggcaat gtcctggatg atgcgccagt cctgacgggc ttcgccgggc gggtcaatga
     1561 ctttattgat tttgatgacc cggccttcga tttgggtgac gattccttca tcttcttcgt
     1621 ggagcgaacc agggagcaca atgtcggcgt gatgcgcggt ttcattcagg aaaaagtcaa
     1681 tcgccacata aaattcaagt ttttcgaggg ctttggtgac aaacgcattg tctggcaggg
     1741 acactttggg gttgaaacag agcgacaaca agccacgaat ctcacccctg tcaattttgc
     1801 ggaaaatttc ataggcatca acgcccgcct ggggaagctc atccggtgaa acaccccaga
     1861 ctttggcgac ataagcacga tgttcggggt tgctgatgtc gcgtccgcca gggagttgat
     1921 cgcatttctg gccatgctcg cgtcccccct ggccattgcc ctggccggta atggtggcat
     1981 atccgcagtt ttcgcgtccg attcggcctg aagccagcac catgttgatg gcaccgagga
     2041 cgttctgaac gccgtggctg tggtgctcga tcccacgggc atgcatcagg aagctggttt
     2101 gggccgtgcc ccaccattcc gctgcctgac gaattgattt ttcggcaatg ccggtgactt
     2161 cagccgtatg tttgggcgtc cacgctttga cgtgcccggc aacagcttca aaaccaaccg
     2221 ttgaattccg aataaagtca tggtcgagcc agtcgttttc aatcattaaa tggaggattc
     2281 cgttaaagag cgcaatatcc cgacccggtt tgattggcag aaacaggtca caggtgcggg
     2341 caatcggcgt gatccgggga tcaaccacga tgattttggc gccgtgttcg cgagcctgcc
     2401 agacataatt ggtcgtgatg ggggcgcatt cggccacgtt ggcgccgctg atccagatct
     2461 gcaggccgaa cggcgtccgg gtgttatcaa
//
LOCUS       DAOQDO010000829         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37056_length_2489_cov_3.796220, whole genome shotgun sequence.
ACCESSION   DAOQDO010000829 DAOQDO010000000
VERSION     DAOQDO010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37056_length_2489_cov_3.796220"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..384)
                     /gene="trxA"
                     /locus_tag="PKZ53_13295"
     CDS             complement(58..384)
                     /gene="trxA"
                     /locus_tag="PKZ53_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNJ41462.1"
                     /translation="MSGSIVELTDGTFKNEVLASDIPVLVDFWAPWCAPCRMLSPTIE
                     ALADDFQGRAVVGKMNVDENNVTPGNYKVQGIPTLILFKAGVEQERLVGATSKDNIAR
                     MINKHL"
     gene            complement(629..2068)
                     /gene="glgA"
                     /locus_tag="PKZ53_13300"
     CDS             complement(629..2068)
                     /gene="glgA"
                     /locus_tag="PKZ53_13300"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNJ41463.1"
                     /translation="MHILLAVSEVAPYAKTGGLADVASALPKALAALGEQVTVVMPRY
                     GFIHTGEEVWYNLPVPFAHTIKHIRLFRENLGKATIYWIDAPEYFSRGKAIYGESDDP
                     ERFAFFCRAVLEVARWLGTPPDIIHCNDWMTGLIPVYLRTVYATDPYFAGTATLLSIH
                     NLAFQGLFSPSQLPRFGLSMELNTTDGGLEFRGTASVLKGGLVATDAISTVSKKYAQE
                     IQTAEYGFKMEGLLHARRHDLIGILNGVDYDIWNPATDKNIAANYSMEDLSGKRVCKA
                     DLLSYFHLPHELDRPLIAVVSRISDQKGFDLIREVSGRMLQAGAFFVLLGSGDSQYED
                     FFQRLRDFAPHRVGVYFGFNNELAHRIEAGADIFLMPSWFEPCGLNQIYSLKYGTVPI
                     VRATGGLDDTIQDFDRITRTGNGFKFRAYRADRLIEKIYEALLLYNDQDLWKSIMLNG
                     MRANHSWEVAAHEYLDVYRILKHTIRERM"
     gene            complement(2211..>2489)
                     /gene="rho"
                     /locus_tag="PKZ53_13305"
     CDS             complement(2211..>2489)
                     /gene="rho"
                     /locus_tag="PKZ53_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681801.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNJ41464.1"
                     /translation="MDDVIFEEFKGTGNMEIHLDRKLVEKRIFPAIDINKSATRKEEL
                     LIPKEDLNRIFVMRRVLSPLSSVESMELITERLERTKSNTEFLASMNT"
BASE COUNT          627 a          607 c          620 g          635 t
ORIGIN      
        1 agatcgcaat tacgggcggt tttcgaaaac ttactgcaag ggcacaaacg tctgatttta
       61 caggtgcttg ttaatcatac gggcaatatt atcttttgat gttgctccaa ccagtcgttc
      121 ttgttcaaca ccagctttga aaagaatcag ggttggaatc ccttgaactt tataatttcc
      181 tggggtgacg ttgttttcat caacattcat ttttccaacc accgcgcggc cttgaaaatc
      241 atccgcgaga gcttcgattg ttggggacag catgcggcaa ggcgcacacc agggcgccca
      301 gaagtccacc aacaccggaa tgtctgacgc taacacttcg tttttgaagg tgccgtcagt
      361 aagttctaca atgctaccac tcatggttct tcctcctaat attgttcaaa tccaaattga
      421 ttgcgaattg ctcacctgcc actcagacgc tgtgcggttg ctctggaatc caggtcggtt
      481 gtgaaatgca aacgagccga tggaccagaa aaaccaggtt gtcactgagc cttcaccaag
      541 gtcaacctcc tcaggttttc tcaatggcgg ttgtgcatga tgttgcatac aagatttaat
      601 gaatgaacgc aagaagtgcc cgcaggcctt acatgcgttc gcgaatcgta tgtttcaaga
      661 tccggtagac atctaaatac tcatgagctg ccacttccca ggagtgattg gctctcatcc
      721 cattgagcat aattgacttc cagagatcct gatcgttata gagcagcaag gcttcgtaaa
      781 ttttttcaat cagccgatct gctcgatagg cacggaattt aaacccattt ccggtccggg
      841 ttattcgatc aaaatcctgg attgtgtcat caagcccgcc agttgcccgc acaatcggga
      901 cagtgccata tttgaggctg taaatctggt tgaggccaca gggttcaaac caggatggca
      961 tcaagaagat atcggcgccg gcttcgattc gatgtgccag ttcattattg aagccaaaat
     1021 agaccccaac gcggtggggg gcaaagtccc gcagtcgctg gaagaagtct tcatattggg
     1081 aatccccaga ccccagcaaa acaaagaacg ccccagcctg gagcattcgg ccagatactt
     1141 cccggattaa atcaaaccct ttctggtcac taatgcgcga taccactgca atcagtgggc
     1201 gatccagttc gtgcgggagg tgaaaatagc tcaggagatc agccttgcac acccgtttgc
     1261 cagacaaatc ttccattgag tagttggccg cgatgttttt gtcggtggct gggttccaga
     1321 tgtcataatc cacgccattc aggatgccta ttaagtcatg gcgacgggca tggagcagtc
     1381 cttccatttt aaacccgtat tcagccgtct ggatttcctg ggcatatttt ttgctcacgg
     1441 tcgagatggc atcggttgcc accagtccgc ctttgagcac gctggcggta ccccggaatt
     1501 caaggccacc atccgtggtg ttgagttcca tggaaagacc aaacctgggc aattgtgagg
     1561 ggctaaacag tccttgaaac gccaggttgt ggatgctgag cagggttgct gtcccggcaa
     1621 agtatggatc tgtggcatat accgttcgca aatataccgg aatcagacca gtcatccagt
     1681 cattgcaatg aatgatgtcg ggtggtgttc ccagccagcg ggcaacctca agcacggccc
     1741 gacagaagaa agcaaaccgt tccggatcat ccgattcacc atagattgct tttccacgtg
     1801 agaaatattc tggggcatca atccagtaaa tggtggcttt tcccaggttt tcccgaaaga
     1861 ggcgaatgtg tttgatggta tgggcaaatg gtaccggcag gttgtaccag acttcttcgc
     1921 cggtatggat gaacccatag cgaggcatga ccaccgtgac ctgttcaccc agagcggcca
     1981 gtgctttggg caatgccgag gcaacatcag ccagcccacc agtttttgca tagggagcaa
     2041 cttcagaaac cgcaagtaaa atgtgcatag gtggtgtttt gtacttggta cttggtgctt
     2101 ggtgcttggt gcttggtgct tggtgcttga aggggaagaa cgtatatgtt cgaagaacaa
     2161 aaacaaaaaa ccaggagcca agaaccgaga accaagaacc aagcaccagt ttaggtattc
     2221 atggaagcca gaaattcggt attgctcttg gtgcgttcca gtcgttcggt gatcaattcc
     2281 attgattcga cgcttgaaag cgggctgagc acccgacgca tcacaaagat ccggttgaga
     2341 tcttccttcg gaatcagcag ttcttcctta cgggttgccg acttgttgat atcaatggct
     2401 gggaagatgc gtttttcaac cagtttgcga tcaagatgga tttccatatt gcctgtgcct
     2461 ttgaattctt cgaagatcac atcatccat
//
LOCUS       DAOQDO010000830         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37062_length_2489_cov_3.071898, whole genome shotgun sequence.
ACCESSION   DAOQDO010000830 DAOQDO010000000
VERSION     DAOQDO010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37062_length_2489_cov_3.071898"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKZ53_13310"
     CDS             <1..346
                     /locus_tag="PKZ53_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41465.1"
                     /translation="KNTLRVARDGVEALALLRQEGPFANACIPDLILLDLNLPKKDGR
                     EVLAEIKKDNRLRSIPVVVLTTSDAEQDILRSYDLHANCYVTKPVDLDQFIQVVESIE
                     GFWLSIVKLPTL"
     gene            359..2185
                     /locus_tag="PKZ53_13315"
     CDS             359..2185
                     /locus_tag="PKZ53_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ41466.1"
                     /translation="MKIETIQVLLIEDHPGDARLVREMLAEAKDIVFSVTYVRLLSEG
                     VTILKQAHVDVVLLDLSLPDSNGLSTFESIQDQFPEIPMVLLTGFEDVSVAIHALNLG
                     AQDYLVKGQVDGSLLERSLRYAIERKRVEEALKKSERRLRSMVENLPAGAVYREGDSI
                     FFNKAVEKITGYQPHEITTISEWFSSLYGGEGKRFAAEYEADRATQFADSRIQAIVRK
                     DGQIRFVEFAGYSYEQGEVWLLYDITERLQAEQLIREQATLLNQSQDAIWVLDRDNRV
                     QFWNQGAEQLFEWKAAEVMGKPLEAVFWDCCQTELVVIQKEIAEKGGWRGELHMATKS
                     GRELIVESRWTVSYDSQSHPQSKLTVNTDISDKKRLENEFMRATQLSLIGELAAGLAH
                     EIKNPLAGIQGAVDILIRRRSPGDPERTALEAVRREVGRIDGTVRELLNRARPRALTQ
                     VPTLLNEIVRHAVVLSRHQATVGPNADRRVAIDLELPTDPIVASIDTAQMEDAILNLI
                     LNALDAVGDRGQITVRLKLVKLESPNRPLEAVIEVEDNGRGIAESDLAKIFHPFYTTS
                     PRGTGLGLPAVRRIARAHGGQVDVKSEIAKGSVFTLHIPLGH"
BASE COUNT          578 a          593 c          708 g          610 t
ORIGIN      
        1 caaaaacaca ttgcgggtcg cccgcgatgg ggttgaagcc ctggccctgc tgcgccagga
       61 agggccattt gctaatgcct gtattcctga cttgattttg ctggatctca atcttcccaa
      121 aaaagacggg cgcgaagtcc tggccgagat caaaaaggat aaccggttgc gaagcattcc
      181 agtggtggtg ttgaccacct cagatgccga gcaggatatt ctgagaagtt atgatctgca
      241 cgccaactgc tatgtcacca aacccgttga ccttgatcaa tttattcagg tggttgaatc
      301 aattgaaggg ttttggctca gcattgtgaa attaccgaca ctgtaaagga agtggtttgt
      361 gaagattgaa accattcagg ttttattgat cgaagaccac ccaggtgacg ctcgcctggt
      421 tcgtgaaatg ctggccgaag ctaaagatat tgtgtttagt gtgacctatg ttcgcttgct
      481 ttccgaaggc gtcactattt taaagcaagc gcacgtggat gtggttctgc ttgatctttc
      541 gttgccagat agcaatggat tgtcaacctt tgaaagcatt caagaccagt ttcctgaaat
      601 accgatggtt ttgctgaccg gatttgaaga tgtttcagtt gccattcatg ccctgaatct
      661 gggggctcag gattatctgg tcaaggggca ggtcgatggc agtttgcttg agcggtcact
      721 caggtatgca attgaacgca aacgggttga ggaagctttg aaaaaaagcg agcgtcggct
      781 tcgatccatg gtcgaaaacc tgccagccgg ggctgtctat cgtgaaggcg attcgatttt
      841 ctttaacaag gctgtcgaaa aaatcacggg ctatcaacca cacgaaatca ccacgatttc
      901 agaatggttt tcctcgctct atggtggtga aggcaaacga tttgccgcag agtatgaggc
      961 agaccgggcg actcagtttg ctgattcgcg aatccaggcg attgtgcgca aggatggaca
     1021 gattcggttt gttgagtttg ccgggtacag ctatgagcag ggtgaggtgt ggttgttgta
     1081 tgacattact gaacgcctgc aagccgaaca attgatccgt gaacaggcga cccttctgaa
     1141 tcagtcacag gatgccattt gggtgctgga ccgggacaat cgggttcagt tttggaatca
     1201 aggtgctgag caattgtttg agtggaaggc cgccgaagtc atgggaaaac cactggaagc
     1261 tgttttttgg gattgctgcc agactgagct ggtcgtcatc cagaaggaga ttgcggaaaa
     1321 agggggctgg cggggcgagt tgcatatggc aaccaagtcc ggaagggaac tgatcgtcga
     1381 aagtcgctgg acagtttcct acgatagtca gagccatccg caatcgaaac ttaccgtcaa
     1441 taccgacatt tccgacaaaa agcggcttga aaatgaattt atgcgggcga ctcagctttc
     1501 gttaatcggc gaactggctg cggggctggc acacgaaatc aaaaacccac tggctggaat
     1561 tcaaggcgcg gtggatattt tgattcggcg gcgctcacca ggtgaccccg aacgtaccgc
     1621 cctcgaagcc gtccgacgtg aagttggtcg aattgatgga acggtgcgtg agctactcaa
     1681 tcgggcccgc cctcgtgccc tcacccaggt tccaaccctg ctcaatgaaa ttgtccggca
     1741 cgccgtggtg ctctccagac atcaggcgac agttgggcca aatgcagatc gtcgggtggc
     1801 cattgatctg gaattgccaa cggacccaat tgttgcctca attgacacgg ctcaaatgga
     1861 agatgccatc ctcaatctga ttttgaatgc cctggatgcg gttggcgacc gtgggcaaat
     1921 tacggttcgg ctcaagctgg tcaagcttga gtctcccaac cgtccactcg aagcggtgat
     1981 tgaagtcgaa gacaatggcc ggggcattgc cgaatcggat ttagccaaga tttttcaccc
     2041 attttacacc acttctccac gcggcaccgg tctgggcctc cccgcggttc gccggattgc
     2101 ccgcgcccac ggcggtcagg ttgatgtgaa atcagaaatt gccaaaggct cagtttttac
     2161 actgcatatt ccacttggcc actaactgac cggatatcaa aaagatgagg aaccgggtgt
     2221 ggagtgcggc ggctcgacgc cgctttcgta ttcggcggct tgacgcctgt ggagtgcggc
     2281 ggctcgacgc cgcttttcag aacggcatcg tcgtcaaccg aagggacttc ttccccgtcg
     2341 gcgaagacat cgcggtcggc gtcggtggac gaacggcggg aatgggttac gggatggact
     2401 cgttgcggca gtgcttttcg gggtaacaag gtgaggtttt ctcacccttc tttgaatgga
     2461 tgacttgtta tttcgctcct tcgcttatt
//
LOCUS       DAOQDO010000831         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37088_length_2488_cov_3.565146, whole genome shotgun sequence.
ACCESSION   DAOQDO010000831 DAOQDO010000000
VERSION     DAOQDO010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37088_length_2488_cov_3.565146"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1187
                     /locus_tag="PKZ53_13320"
     CDS             306..1187
                     /locus_tag="PKZ53_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41467.1"
                     /translation="MGSTDAPQLNIGQHWLCPEPTTTATFTHYRPAVFLECQLRFHHL
                     RAHIKHTAEKRYTAWYPRGQASVDWDIPALGDTEVLNIAMGPPHHLPHKTENFDFSDQ
                     NFREFIEEKLNKLVRTARLKVFHNAHFDVFSEPDETRDHFITRLAEKAPDSIENDLNN
                     LIRRVNMKISQVRETQERRGRKLEIPEMFINDILMERRHELFASQYRLEALFSSGEKL
                     LVTSSDGIASFPINDPEYIEMMETLQLIEDETKRELNELCTRCIQKVSECDLFEIGLQ
                     RNQIHILRKGVLWVPTA"
     gene            complement(1364..2104)
                     /locus_tag="PKZ53_13325"
     CDS             complement(1364..2104)
                     /locus_tag="PKZ53_13325"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41468.1"
                     /translation="MNENLNEDVKTPVSAEPELGAEPEPEAESGIDPELDAVSALAAK
                     IEADTEPLPPSTNPKGDQDLVLRCRAGDAAAWEGLVRLYSRRIYNLAYRFVSRHEQAE
                     DLTQDVFMRIYKSLEQYNPEMGDLSNWLMRLARNLIIDDYRRRQRQPLDGGDDLEDHT
                     HYLRSRTDNPHHSIERKERRLQVFDAINKLSPDLRQCVIMRDIDELTYQEIVDRLQIP
                     EGTVKSRINRGRIELAKVLRRMKVVSID"
BASE COUNT          654 a          634 c          605 g          595 t
ORIGIN      
        1 accaagcacc aagcaccaag taccaagtac caagcaccaa gcaccaagca ccatcacgcc
       61 ttgcaataat tgcccggagt cgccaaactg aaggtgtttt ctgtctcttc tcttgccact
      121 taaagccttc ttcccctcgc gctttcaccc aggcgaaccc aaatgagtgg cctccatcta
      181 tttgaaatca aaagggtttc atctcgaaac ctggatcgcg tcaaacgaac caaaaatttt
      241 aattgatcga gagcgggatt ggtgtatccg catctggtct ttcaaccagg agtgagcaag
      301 gaattatggg gtcaacagat gcaccgcaac tcaacatcgg gcaacactgg ctttgccctg
      361 agccaacaac aacagcaacc tttacacact atcgcccggc ggttttttta gagtgtcaac
      421 tccgatttca tcacttgcgg gcccatatca aacatacagc agaaaaacgc tataccgcgt
      481 ggtatcccag gggacaggca tcggttgact gggatatccc agctctcggc gatacggagg
      541 tcctgaatat cgccatgggg ccacctcatc atctgcccca taagactgaa aactttgatt
      601 tctctgacca aaacttccgc gagtttatcg aagaaaaact caacaagctg gtgcgtaccg
      661 cccggctgaa agtttttcat aatgcacact ttgatgtttt ttctgaaccg gatgaaaccc
      721 gcgaccattt catcacccga ctggctgaaa aagcgcccga cagtattgaa aatgacctga
      781 ataacctgat caggcgggtt aatatgaaaa tttctcaggt tcgggaaacg caggaacgac
      841 gcggacgcaa acttgaaatc cccgaaatgt tcatcaatga catcctgatg gaacgtcggc
      901 acgaattgtt tgccagccag taccggctgg aggctttgtt ttcgagtggg gaaaaattgc
      961 tggtcaccag tagcgacggg attgccagtt tcccgatcaa tgacccggaa tacattgaaa
     1021 tgatggaaac gctgcaactt atcgaagacg aaaccaaacg cgaactcaat gaactctgca
     1081 cccggtgcat tcaaaaagtt tcggaatgtg acttgtttga aattggtttg cagcgcaatc
     1141 aaatccatat tttgcgcaaa ggcgtgctgt gggtgccaac ggcttaacgg catcgggctg
     1201 aagactttgg gctaggggct gaagaagtca ggctgaaaga catcgggctg aagaagttgg
     1261 ggttcaaccc attttcttca gcccgttttc tttaacccga tgtcttccgc cccgagcttg
     1321 cgagtcttca gccccgagcc ctcaaccctc agcccaggga tttttaatca atactcacaa
     1381 ctttcattcg ccggagcact tttgccagct caatccgtcc ccgattgatt cgtgatttga
     1441 cggttccttc tgggatttgc aaccgatcta caatttcctg ataggtcaat tcatcaatgt
     1501 cacgcatgat gacgcactgg cgcaggtcag gcgagagttt attgatggcg tcaaagacct
     1561 gaagtcggcg ttctttgcgt tcgatactgt ggtgaggatt gtcggttcgg cttcgcaggt
     1621 aatgggtgtg gtcttctaaa tcatcgccac cgtcgagcgg ctggcgctgg cggcggcggt
     1681 aatcatcaat aatcaaattg cgggccaggc gcatcaacca gttgctcaaa tcgcccattt
     1741 ccggattgta ctgttcgagc gatttgtaaa tccgcataaa cacatcctgg gtcagatctt
     1801 cagcctgttc gtggcggctg acaaaccggt aggccagatt atagatgcgt cgtgaatata
     1861 accggaccag cccttcccag gcagccgcat ccccagcccg acagcggagt accagatcct
     1921 gatctccctt tggattggtg cttgggggaa gtggctcggt atcagcttca atttttgctg
     1981 ccaatgcgga cacagcgtcc aattcaggat caatccctga ttctgcttcc ggttctggct
     2041 cagcccccag ctctggttca gccgaaaccg gggttttcac atcctcattg agattctcat
     2101 tcataaccag ttggcgatgc ggctgcgctc tatagaaatt atgaatgatg agttacgaat
     2161 tatgaagaaa ccacctgatt gacgaccaga tggttcgcgg tttcatcatt caaaattcat
     2221 cattcgaaat cagtatcgtg gtggaaagaa gatttgtttg tgttccctgt gcaataagag
     2281 cttaaaatat cacgcttgtc gagtgcaaac aaagtggttc ttcacgctga aagtgaagat
     2341 agttgagaag gagggcgaaa ggctggcgcg tatggggact gaggctgaga gtaggaaaag
     2401 gacagaaaca gcagaaaaaa tggcaaggca atcagttcga aaacccggaa cccggaaccc
     2461 ggaacccgga acccggaacc cggaaccc
//
LOCUS       DAOQDO010000832         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37158_length_2486_cov_2.538050, whole genome shotgun sequence.
ACCESSION   DAOQDO010000832 DAOQDO010000000
VERSION     DAOQDO010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37158_length_2486_cov_2.538050"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PKZ53_13330"
     CDS             complement(<1..761)
                     /locus_tag="PKZ53_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008278051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNJ41469.1"
                     /translation="MPASGRAFAIGLSLNVLFVMVEFLFGFWSGSLALVADAGHNLTD
                     VAGLLLAWAGIWLARQKPTVNHTYGFRRSTILAAFFNALLLIVTVGAIVWEALSRLKS
                     PQAIHEGIVIGVAALGIVINTTTALFFATGQKNDLNLRGAFIHMAADAVVSLGVVLSG
                     LLIWKTNWYWLDPVVSLVISVVILVSTIGLLRDSLNLALDAVPGTINIVEVRAYLLNL
                     PTCREVHDLHIWAMSTTEIALTAHLVMDRTACNDVL"
     gene            complement(1234..1434)
                     /locus_tag="PKZ53_13335"
     CDS             complement(1234..1434)
                     /locus_tag="PKZ53_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001909668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41470.1"
                     /translation="MKHSQFFILHSSFFILHSSFLILNSSFFILNSSFFILNSSFFIE
                     IPGPKRFDFTNRFQPKGFPWPG"
     gene            complement(1491..1964)
                     /locus_tag="PKZ53_13340"
     CDS             complement(1491..1964)
                     /locus_tag="PKZ53_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41471.1"
                     /translation="MNKKGFWSWLSLTGMALTLATGLGYVHFAEVVSVDAAGKPAKPA
                     VSAQKPGDWKELKAFHSVMSNTFHPMEEGDYKPIRARASEMAQKAKEWADSTPPKDFD
                     KASIKETLKKLVVETKALSDKIAKNASDEEIKKDLTALHDRFHEVVGLCRAEDHE"
     gene            2055..>2486
                     /locus_tag="PKZ53_13345"
     CDS             2055..>2486
                     /locus_tag="PKZ53_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41472.1"
                     /translation="MHTIVESPILINRVFFSGTVRMLLAVTWMLFLAGQSVQAGPKLP
                     SPNDLLKRYTKASGGDKLLKPLRQTSYQGTALTADGQKGTFSLDLEAPNRRRFELNFD
                     ADNRLELATGTSVWMRSTRGGDTTLIDEAARQLKAEVTVLAN"
BASE COUNT          679 a          608 c          587 g          612 t
ORIGIN      
        1 aggacatcgt tgcacgccgt tcgatccata accagatgcg cggtcaatgc aatttcagtg
       61 gtgctcatgg cccagatgtg cagatcgtga acttcccgac aggtcggcag attcagcaga
      121 taggctcgca cttcaacaat gtttatcgtg ccgggaactg catccagcgc cagatttaac
      181 gagtcacgca ataacccgat ggtactgacc aaaataacaa cggaaatgac cagactgacc
      241 actggatcca gccagtacca gttggttttc caaatcaata gccctgagag aacgacacca
      301 agtgaaacca ccgcatcggc ggccatgtgg ataaacgctc cacgcaggtt gaggtcattt
      361 ttttgcccgg tggcaaaaaa cagcgcagtc gtggtattga ttacaattcc aagggcagcg
      421 accccgatta caatgccttc gtgaatggcc tgtggagatt tcagccgact cagagcctcc
      481 cagacaatag cgccaaccgt cacaattaac agcaaggcat tgaaaaacgc ggccagaatc
      541 gtagaccgtc gaaagccata agtgtgattg acagtcggct tttgtcgtgc cagccagatt
      601 ccagcccagg caagcagtaa cccggcaacg tccgtcagat tgtgtcccgc atcagccacc
      661 aacgccagcg agcctgacca aaagccaaag agaaactcaa ccatcacaaa caagacattg
      721 agactcaacc caatggcaaa ggctcggcca gaggcaggca tagatgaatg gtggtgatgg
      781 tgctcatgtg ccatgaaaaa accggcttct cctgaatcaa aatttttgat caacccaaac
      841 ctgaatattt ctcactttga tcggtaccgt ccagcacttt caaccatcac tgcctgattt
      901 tcaccttgat cacttcattt ttttcgaaga cggcgcagaa actccgggtc aaatgtagcc
      961 ttgctgttac aaagtgttcg atttctgtta catgttgaac ctgattgcac tgtaataatg
     1021 ggaaaccctc tgctttgttt ggtttccagg aatattccag aggaaatagt tgaagccagc
     1081 caaacctttt cgattatttc ctttaagtag gttatatgga acgttttacc aacattgaac
     1141 aaatcagaat tctcaacttt tcgagcatgg gttagcagga atagtgtcgg ggttatcctg
     1201 gctagaaaaa gcttaccagg aatagtgtcg gggttatcct ggccagggaa accccttggg
     1261 ctgaaaccgg ttggtaaagt caaaccgttt tggtccagga atttcaatga agaatgagga
     1321 attgagaatg aagaatgagg aattgagaat gaagaatgag gaattgagaa tgaggaatga
     1381 agaatgaaga atgaagaatg aagaatgaag aatgaagaat tgagaatgtt tcatccctca
     1441 tccttcatcc ctcatccctt cgattgctct gagcttgcga gtcttcagcc ttactcgtga
     1501 tcttcagccc ggcacagacc gacgacttcg tggaatcggt cgtggagggc ggtcaaatcc
     1561 tttttgattt cttcatcact ggcattttta gcgattttgt cagaaagggc tttggtttcc
     1621 acaaccagtt ttttcagggt ttctttgatg ctggctttat caaagtcctt gggaggtgtt
     1681 gaatcagccc attctttggc tttttgggcc atttcactcg ctcgggcgcg aatgggctta
     1741 tagtcgcctt cctccatggg atggaaggtg tttgacataa cactgtgaaa ggctttgagt
     1801 tctttccaat caccgggttt ttgcgccgaa actgccggtt tggctggttt cccagccgca
     1861 tccactgata caacctctgc gaaatgaacg tatccaagac ctgtggccaa tgtcagggcc
     1921 attccggtca agcttaacca actccaaaat ccttttttat tcatggggaa gaagttcctt
     1981 tactgcgaat ttttcaagaa ataaggggca aagtgttcgc gatgatcaat cacgaaacta
     2041 aagtgagaaa aagaatgcac acaatagtgg aatcaccgat tttgatcaac cgggtgtttt
     2101 tttcaggcac tgtccggatg ctgctggccg taacctggat gcttttcctg gcaggccaat
     2161 ctgtccaggc aggacccaaa ctcccgtctc caaatgacct gctcaaacgc tacaccaaag
     2221 ccagtggtgg cgacaaactc ctcaaaccac tccgccagac cagttaccag ggaacagcgc
     2281 tcacggcaga tgggcaaaaa ggcacatttt cccttgatct agaagccccc aaccgtcgcc
     2341 ggtttgaact caattttgat gctgacaacc ggttggaact tgcaacggga acatcggtct
     2401 ggatgcgctc gactcgtggc ggagacacca cattgattga cgaagccgcc cgccaactca
     2461 aagctgaagt cacggttttg gccaat
//
LOCUS       DAOQDO010000833         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37302_length_2480_cov_2.607835, whole genome shotgun sequence.
ACCESSION   DAOQDO010000833 DAOQDO010000000
VERSION     DAOQDO010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37302_length_2480_cov_2.607835"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PKZ53_13350"
     CDS             complement(<1..2071)
                     /locus_tag="PKZ53_13350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ41473.1"
                     /translation="MSHRPLHSSDLFQSGHPWRTIRRILVGFACFLVVTINPVSAQKP
                     APQKPPVAPRPKNAAEPTTDKRSTPAPKTTEAQTELETIEAQFSKKSCTELLAEVGNA
                     NPLMRLTAIRFLKDCLGPAETREEVEKVLLEQLKSDAWMIRGEAAFSLGFGTVSPVVS
                     TALVQALKDDHWFVRARAVQAVGQLKIQAAVPDLVQLFGKESWDTRARVVYALGQIGD
                     SRRIDVLLTAMNSEDESLRLVALQALKNLRGERAKSALLQAIATEKTAEGKLQLVRAV
                     GNLRLVEAKDQVTSWRGESLEFDRELTLTRYKLGETGETEALIRDFGLYPMAVQQELI
                     ADWTTKREALAGPLMLTLLADTASPHRQAAASALEALGEKVPVSGLLQALVDVDPPTR
                     EILVRMLRATHDSSLPELIIARLKDEPPPPEREALLTVLDDFKTSQTIDALLAARLDD
                     KQKPRQAINLALERFNVTLEVLAGRAANAELSPEQRIEATAWLGQLGEATAVTKLTEL
                     LKSSDTRQRLAAVEALGKVEDRTAIDALIVSLEDPVLEIRTAARTGLSRFGVTPERMV
                     ADLKSENWQIRYEALRLLSQLGGAEHSPAIVPLLNDPESLIRRTAATVVGEFKETRAI
                     EPLLALLRDSDLTVRAAAASALGVIGDHRAATGLLAALKTTTDATVSLEIASALAKLK
                     NTQAVPVL"
     gene            complement(2091..>2480)
                     /gene="folK"
                     /locus_tag="PKZ53_13355"
     CDS             complement(2091..>2480)
                     /gene="folK"
                     /locus_tag="PKZ53_13355"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNJ41474.1"
                     /translation="YLDQPWFLNTVVELTFSEPVSPFIFLAECLALETEFGRVRTVPK
                     GARTLDIDLLLCRTAQHYVTCDEYRDGVWLTLPHPRLHLRRFVLEPLCDLCPDAIHPG
                     LGKSFRTLLSELEDESIVRRVTDDLFC"
BASE COUNT          554 a          714 c          660 g          552 t
ORIGIN      
        1 gcaaaaccgg caccgcctgg gtgtttttca atttggccag ggccgacgca atttcaaggc
       61 tcaccgtggc atcggtggtg gtcttcaacg ctgccagcaa tcctgtggcg gcccgatgat
      121 cgccaatcac ccctaaagct gaggccgccg ccgctcgcac ggttaaatca ctatcgcgca
      181 aaagcgccag cagcggttca atcgcacgcg tttctttgaa ctcacccacc acagtggcgg
      241 ctgtgcgccg gatcagactt tccggatcat tgagcaacgg cacaatcgcc ggactgtgct
      301 cggccccacc aagctggctc aacagccgca atgcttcata gcgaatctgc cagttttctg
      361 atttgagatc agccaccatc cgttctggag tgaccccaaa ccgtgaaagc ccggttctgg
      421 ctgcggttcg gatttctagc actggatctt caagactcac aatcagcgca tcaatcgcag
      481 tgcgatcttc aacttttcca agggcttcga cagccgccag ccgttgtcga gtgtcgcttg
      541 atttgagcaa ctcagtcagt ttggtcacgg cggtggcttc accgagttgc ccgagccacg
      601 ccgtagcctc aattcgctgt tcgggtgaaa gttcagcgtt cgctgcccgg ccagccagca
      661 cttcgagtgt cacattgaat cgttcaagtg cgagattgat cgcctgacgt ggcttttgct
      721 tgtcgtccag gcgggctgcc agcagggcat caatcgtttg cgaggtcttg aaatcatcca
      781 gtacagtgag caatgcctcg cgctctggcg gtgggggttc atctttgagc cgtgcaatga
      841 tgagttcggg gagcgatgaa tcgtgggtgg cgcgcagcat ccggaccaga atttcccgcg
      901 tcggtggatc aacatccacc agcgcctgga gtaatccgga cacgggaact ttttcaccaa
      961 gggcttcaag tgccgaagcc gctgcctgtc ggtgtggtga agccgtatca gccagcaagg
     1021 tcaacatcaa cggcccggcc agcgcctcgc gtttggtcgt ccaatcagca atcaattcct
     1081 gctgaaccgc cattggatac agtccaaaat cccgaatgag cgcctcggtt tcgcctgttt
     1141 cacctaattt gtagcgtgtc agggtcagtt cccggtcaaa ttcaagcgat tcacctcgcc
     1201 acgaagtcac ctgatctttt gcttcaacca gcctgagatt tccaactgcc cggacaagct
     1261 ggagttttcc ttcagccgtt ttttcagtcg caattgcctg caaaagcgca ctttttgccc
     1321 gttcgccacg caggtttttg agtgcctgca gggccaccag tcgcaggctt tcatcttcgc
     1381 tgttcatggc cgtcaataac acgtcaatgc gacgcgagtc gccaatctgg ccaagggcat
     1441 acaccacccg cgcccgtgta tcccaggatt ctttgccaaa caattgaacc aggtcgggca
     1501 cagcagcctg gattttgagc tggccgacgg cctgaaccgc ccgcgcccgg acaaaccagt
     1561 gatcatcctt gagtgcctgc accagtgcgg tgcttactac cggcgaaacg gtgccaaatc
     1621 cgagtgaaaa cgcagcttcg ccacggatca tccaggcatc actcttaagt tgctccagca
     1681 ataccttttc gacttcctca cgggtttcag ccgggccaag acaatccttc aaaaaccgaa
     1741 tcgcggttaa tcgcatgagt ggattggcat taccgacttc ggccagcaat tcggtacagg
     1801 actttttgga aaactgagct tcgatggttt cgagttcggt ctgggcttcg gtggttttcg
     1861 gagccggcgt tgaacgtttg tcagtggtgg gttccgccgc attttttggg cgcggcgcaa
     1921 ccggcggctt ttgcggggct ggcttctgtg ccgacaccgg gttgatcgtc acaaccagaa
     1981 aacaggcaaa tccaaccagt attcgccgaa tcgttcgcca cggatggccg gactgaaaaa
     2041 gatcagagga gtgcaaagga cgatgtgaca tcacagaaaa acaacttctt tcagcagaat
     2101 aaatcgtctg tcacccggcg cacgattgac tcatcctcaa gctcggaaag caacgtgcga
     2161 aatgacttgc ccagccctgg gtgaattgca tctggacaca gatcacagag cggttccaga
     2221 acaaaccgcc gcaggtgcaa acgtgggtgc ggcagtgtca accagacacc gtcgcggtat
     2281 tcgtcacatg taacatagtg ctgtgctgtt cgacaaagca gcaaatcaat atcgagtgtc
     2341 cgggccccct tgggaacggt gcgaacccgg ccaaattcgg tttccagggc caggcattca
     2401 gccagaaaaa tgaacgggga aacaggttca gaaaaagtca gctccaccac tgtatttaag
     2461 aaccagggtt gatcaagata
//
LOCUS       DAOQDO010000834         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37379_length_2477_cov_3.266309, whole genome shotgun sequence.
ACCESSION   DAOQDO010000834 DAOQDO010000000
VERSION     DAOQDO010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37379_length_2477_cov_3.266309"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="PKZ53_13360"
     CDS             <1..1879
                     /locus_tag="PKZ53_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNJ41475.1"
                     /translation="LDPYALEMSHDPTTPTNTDGTIYASGATYRNINSGPKAPKGIVL
                     SLDTTSFGTKPTRALKDDIVYEVHVRGLTKNDPSIPAAYQGTYKGAALKAQALADLGI
                     TAVEFLPVQETQNDTNDVNPTTTAGDNYWGYMTLNYFAPDRRYSSDKTPGGPTREFKE
                     MVKAFHDRGIKVFIDVVYNHTGEGGAWNGNDPTVFNVMSWRGLDNPTYYCLTSDKQFS
                     WDNTGVGGNYNTFNPVAQDMIVDSLAYWRDTIGLDGYRFDLASVLGNTCEHGCFNYDK
                     LNSGTALNRIARELTPRPAGGGAGVDLIAEPWAIGGNSYQVGNFPSGWSEWNGIYRDV
                     IRKDQNQMGLESVTPGQLATRFAGSSDLYADDGRKPWNSVNFIVAHDGFTLKDLYSYN
                     SKVNNQAWPYGPSDGGEDNNHSWDQAGWAHDQRKAARNGLALMMLNAGVPMITGGDEF
                     LRTQFGNNNAYNLDSNANWLNYTLSTDQQNFKAFTQGMMAFRKAHPALRPANFYASND
                     TNGNVMEQMRWFRPDGGVADGAYMDNANNHCLAYRVDGTEFGDSASAIYVAYNGWSGD
                     VNFTLPWPGAGKNWYRVTDTSVWAEGATQVRTPGAEDFIGGEYYQYSLKARGILVLIA
                     K"
     gene            complement(2079..2387)
                     /locus_tag="PKZ53_13365"
     CDS             complement(2079..2387)
                     /locus_tag="PKZ53_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41476.1"
                     /translation="MTHEEMERTMEFILAQQARFSVDIDLLKESIVEMKIQAELDRQS
                     AKEDRELMKAMFKHLSENADADRRVMREMIQDLKDTIYRVESKADDALWLARKKEDNA
                     "
BASE COUNT          612 a          653 c          621 g          591 t
ORIGIN      
        1 gctcgatcca tatgcgctgg aaatgagcca cgatccgacc accccgacca acactgacgg
       61 cacgatttat gcctccggcg ctacgtatcg aaatatcaac agtggcccga aagcacccaa
      121 agggattgtc ctctcacttg ataccaccag ctttggcacg aagccaactc gtgcgctcaa
      181 agatgacatc gtgtacgaag tccacgtccg gggcttgacg aaaaatgacc cgagcatccc
      241 ggctgcctat caagggacgt acaaaggggc cgcgctgaaa gcacaggcgc tggcggatct
      301 cggaattacg gcggttgaat tcctgcccgt ccaggaaacc caaaacgaca ccaacgatgt
      361 caacccgacc acgacggcgg gcgataacta ctggggctat atgacgctca attactttgc
      421 cccagaccgt cgctattcat cagataaaac gccgggcgga cctacccgcg aattcaaaga
      481 aatggtcaaa gccttccatg atcgtggcat caaagtcttt attgacgtgg tgtataacca
      541 cacgggcgaa ggcggcgcat ggaacggcaa tgacccaact gtgtttaatg tgatgtcgtg
      601 gcgcggatta gacaacccga cctactactg tttgacgagc gataagcaat tttcctggga
      661 taacacaggt gtcggcggaa actacaacac gttcaacccg gtggctcagg atatgatcgt
      721 tgattcgctg gcttactggc gagacaccat cgggctcgat ggctatcggt ttgacctggc
      781 ttcggtgctt ggaaatacct gtgaacacgg atgttttaac tacgacaaat tgaattccgg
      841 aaccgcgctc aaccggattg cccgcgaatt aaccccacgt ccagcgggtg gtggtgccgg
      901 tgttgatctg atcgccgaac cctgggcaat tggcggtaat tcctatcagg taggcaattt
      961 cccttcgggc tggtccgaat ggaacggcat ttatcgcgat gtgatccgca aagaccagaa
     1021 ccagatgggt cttgaatcag tgacgcccgg acaactggcc acccgctttg ctggttcatc
     1081 ggacctgtat gccgatgatg gtcgcaaacc gtggaattca gtcaatttca ttgtggctca
     1141 cgacggcttt accctcaaag atctctattc atacaacagc aaagttaaca atcaggcgtg
     1201 gccgtatggt ccatcagatg gtggtgaaga caacaaccat agctgggatc aggccggatg
     1261 ggcgcacgat caacgcaaag cggcccgcaa tggactggcc ttgatgatgc tcaatgctgg
     1321 tgttccaatg attactggcg gtgacgagtt cctccgcacc cagtttggca acaacaacgc
     1381 gtacaacctt gattcaaacg ccaactggtt aaactacacc ctgtcaaccg atcagcagaa
     1441 tttcaaagct tttacccagg gaatgatggc tttccgcaaa gcccacccag ccctgcgacc
     1501 agccaacttt tatgccagca atgacaccaa cggcaatgtg atggaacaga tgcgctggtt
     1561 ccgccccgat ggtggcgtgg cagatggcgc ctatatggac aatgccaaca accattgcct
     1621 ggcctatcgg gttgatggca ctgaatttgg agactccgcc tcggccattt atgtcgctta
     1681 caatggctgg tcgggagatg tcaacttcac cctgccgtgg ccaggtgctg gcaaaaactg
     1741 gtatcgcgtc acagatactt cggtttgggc ggaaggcgcc acccaggtgc gcaccccagg
     1801 cgccgaagat ttcatcggcg gcgaatatta ccagtattcg ttgaaagcac ggggcatcct
     1861 ggtattgatt gcgaagtagt tcctgacggt tatttggttc gcttttgaat cacaagagcc
     1921 tgggtttaag gaattaatct cacctggaga ttaaagaaaa ttcctgaagt cccaggttct
     1981 tttttatgag gggaaaaaag gttccgagct gaaaagagaa cctggaaggt cgtgaattaa
     2041 gtcctgtttt ttggtaaatg tacccaaaaa taaactcgtc aggcgttgtc ttcctttttc
     2101 cgggcaagcc atagcgcatc gtcagctttg gattcaaccc ggtaaatggt gtccttcaag
     2161 tcctggatca tttctcgcat tactcttcta tcagcgtctg cattctcaga aaggtgctta
     2221 aacattgcct tcattaactc ccgatcttct ttagctgatt gtcggtccaa ttcggcctgg
     2281 attttcattt cgacaatgga ttccttgagc aaatcaatat ccacactgaa cctggcctgc
     2341 tgggccagaa taaattccat tgtgcgctcc atttcctcgt gagtcatttt ctgttctcca
     2401 tagattgtct ttcttaaaag aattccttct gtcacaaatc ctgtgagtca gtaccacccg
     2461 cgtcagtggg tgggccg
//
LOCUS       DAOQDO010000835         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37388_length_2477_cov_2.567713, whole genome shotgun sequence.
ACCESSION   DAOQDO010000835 DAOQDO010000000
VERSION     DAOQDO010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37388_length_2477_cov_2.567713"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="PKZ53_13370"
     CDS             complement(<1..2379)
                     /locus_tag="PKZ53_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ41477.1"
                     /translation="MIDKIIEWCAHNKFLVLVFIVLAVLAGVHAMRNIRLDAIPDLSD
                     TQVIIYSRWDRSPDIMEDQVTYPIIRSMLGVPKVKDIRGFSDFGFSYVYIIFEEGTDI
                     YWARSRTLEYLNNILPKLPPGVNVELARDETAVGWVYQYVLVDETGKRNLAELRSLQD
                     WHLRYELQSVPGVAEVAPLGGFVRQYQINLDPNSLLGYKIPIDKVIETIREGNNDIGG
                     RLIEFSGREYMVRGRGYIRSVADIENIVVGTNPETGTPILMKNLGTVVLGPDIRRGVG
                     EHNGRGEAVGAIVIMRVGENALKVIDRVKSKLADIESSLPPGVKIVATYDRSELIERS
                     IETLKGTLIEELIIVSLVILIFLWHIPSAIIPIITIPVAIIIAFIPMQQMEITANIMS
                     LGGIAIAIGAMVDAAIVVVEQTHKKLEHWQAEGRPGDFHTVVISAVKEVGGPSFFALL
                     VIAVSFIPVFALEAQEGRLFKPLAYTKTFSMVIAAVLAITLDPAVRLMFTRMDNFTFR
                     PRWLCRLVNSVLIGKIHSEETHPISRPLMRIYHPVVEFVLRHQWMTIVSAVLIVALTV
                     PIYYRLGSEFMPALDEGSLLYMPTMLPGISVTEAQKLLQVQDKLIKSFPEVQSVHGKA
                     GRAETATDPAPFSMMETVIVLKPPSEWPKIPRWYSDWSPTWLQDWLRRAWPDHKSTQE
                     LIYGPGGLNETLQIPGTVNAWTMPIKARIDMLTTGVRTPIGIKVLGPDLNKIEEIGQH
                     IEAVVKEVPGTTSVFAERTAGGYFLDFNLKREELARYGLSVGQAEMVIMSAIG"
BASE COUNT          626 a          620 c          591 g          640 t
ORIGIN      
        1 tccaatggct gacataatca ccatttcggc ttgtccaacc gacaacccat agcgggcaag
       61 ttcttcccgc tttaaattga aatccagaaa atatccacca gcggttcgtt cggcaaaaac
      121 actggttgta ccaggaactt ctttgacaac agcttcaata tgttgcccaa tttcttcgat
      181 tttgtttaag tctgggccaa gaactttgat tccaattgga gtgcgaactc cagtcgtcag
      241 catatcaatg cgcgctttga tcggcatcgt ccaggcattc acggtccctg ggatttgcaa
      301 ggtttcattg agccctccag gaccatagat caattcctga gtgcttttgt ggtcaggcca
      361 ggctcggcga agccaatcct gtaaccaggt tggtgaccag tcagaatacc agcgtggtat
      421 tttgggccat tcagagggtg gtttaagcac aatgacggtt tccatcattg aaaaaggggc
      481 cggatcggtt gcggtttccg cacgccctgc tttgccatgc acactttgca cttctggaaa
      541 cgacttgatg agtttgtctt ggacctggag aagtttttgg gcctcagtca cggaaatgcc
      601 tggaagcatg gtgggcatat agagcagtga gccttcatcc aaagctggca tgaattctga
      661 tcccaaccgg taatagattg gcacggtaag tgctacaatt aataccgccg atacgatggt
      721 catccattga tgccgaagca caaactcgac caccggatga taaatccgca taagcgggcg
      781 gctaatggga tgagtttctt cactgtgaat ttttccaatc aggacgctgt tcaccaggcg
      841 acacagccaa cgtgggcgaa aggtgaagtt atccatgcgg gtgaacatca atcgtaccgc
      901 agggtcaagt gtaattgcca gcaccgccgc gatgaccatg gaaaatgttt tagtataggc
      961 caggggttta aagagtcgcc cttcctgggc ttccaaagca aagactggaa taaaactaac
     1021 agcgatcacg agtagggcaa agaagctggg gccaccgact tccttcacgg cacttatgac
     1081 aacggtatgg aaatcccctg ggcgtccctc agcctgccag tgttccaatt ttttgtgggt
     1141 ttgctccacc acgacaatcg ccgcatccac cattgcaccg atggcaatgg cgattccgcc
     1201 caatgacata atgttggctg ttatctccat ctgctgcatc ggaataaagg caataatgat
     1261 tgctacgggg atggtaatga taggaataat agcgctggga atgtgccaaa ggaaaattag
     1321 aatcaccagg ctgacgatga tgagttcctc aatgagcgtg cctttcaatg tttcaatgga
     1381 tcgctcaatg agctctgacc gatcataggt agcgacaatt ttcaccccgg gtggaagact
     1441 cgactcgata tcagccaatt ttgattttac gcgatcaatg actttgagtg cattttcccc
     1501 gactcgcatg atcacaatgg cgccgaccgc ttctccccgt ccattgtgtt ctccaacgcc
     1561 gcgccgaata tcgggtccca agactaccgt gcccaggttt ttcatcagga tgggggttcc
     1621 ggtttcagga ttggttccga cgacaatgtt ttcaatatca gccaccgagc gaatgtagcc
     1681 acgaccacga accatgtatt cccgccccga gaactctatc aagcgcccac cgatatcatt
     1741 attgccctcc cgaatggttt caatcacttt atcaatcggg atcttgtatc cgagcaatga
     1801 gttgggatcg agattaattt ggtattgtcg aacgaaccca cccagcggag caacttcagc
     1861 cactcctgga acggattgca attcataccg caaatgccaa tcttgaagtg agcgtagttc
     1921 cgccaggttg cgttttccag tttcatcaac caacacgtat tgatacaccc atccgacagc
     1981 cgtttcatct cgtgcgagtt caacattgac gcctggtggt agtttgggga ggatgttgtt
     2041 gagatattcc aatgtgcgtg accgtgccca gtaaatgtcc gttccttctt caaaaataat
     2101 ataaacgtat gagaacccaa aatcagaaaa ccctcgaata tccttgacct ttggcacgcc
     2161 gagcattgat cggatgatcg ggtatgtgac ctgatcctcc atgatatctg gacttcgatc
     2221 ccaccgtgaa taaataatga cctgcgtatc cgacaaatcc ggaatggcat ccagccgaat
     2281 atttcgcatc gcgtgtaccc ctgcaagcac ggcgagcaca atgaaaacca gcaccagaaa
     2341 tttattatgg gcacaccatt cgattatttt gtcaatcata ggttctcgcc tttattggga
     2401 tttatgtcca gaatggtcgg ctgttttttt gtcccgttgg ggctctgatg gtttgggctg
     2461 ggaatggttc attccag
//
LOCUS       DAOQDO010000836         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37413_length_2476_cov_2.767451, whole genome shotgun sequence.
ACCESSION   DAOQDO010000836 DAOQDO010000000
VERSION     DAOQDO010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37413_length_2476_cov_2.767451"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKZ53_13375"
     CDS             <1..180
                     /locus_tag="PKZ53_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41478.1"
                     /translation="PDELPTTDPQASAAKQDFFKLVSSSNEVGILFTGLPPAGDQLSR
                     FQRFGKIIQFQSLVN"
     gene            complement(309..1103)
                     /locus_tag="PKZ53_13380"
     CDS             complement(309..1103)
                     /locus_tag="PKZ53_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNJ41479.1"
                     /translation="MDDLYQILGVSRTATEREIRSAFRRLARQHHPDVSHAPEAKATF
                     QKVAEAYQVLGNPQSRRQYDRGEYIPRRGGRRSNQPEVDPDRERAQRAAAYNSRINRV
                     VDEIIEEERREMRKRADAISVVATLFASAFVVAAARPSTVEPFGLVGHVLVFMLACVA
                     FWQVVKFLREVLIHYTYSPDLILSITSSDDSPHQPFTRASAVVFLALGYVIAIVIGNA
                     IGSVTFVMPEEKVTFLDMVRTGLVYPPIAVCIVVYFRRIGQFLDEW"
     gene            1412..>2476
                     /locus_tag="PKZ53_13385"
     CDS             1412..>2476
                     /locus_tag="PKZ53_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41480.1"
                     /translation="MKRTYGNLLFFWSILLLIAGWSVPVQAENQFWIIGPAITDPAIN
                     TATESHYLYLSRSVQHRNQLFLFFPGTGAIPRNYLKICNTAADMGYHVINLRYPNAEA
                     VNQLCGTRADLDCYEQVRAEVIDGTDRSPLVQVNRANSIENRLIKLLQYMERTYPGDG
                     WGQYLDADGQPRWDLIVTSGHSQGGGHAALLAKYHRVARVVMFAAMDFNFLLGKPANW
                     IGTNNATPTSEYYAFSHERDELVSFSILSNSIYPAYGMTAFGPVTSVEATPLPFENTH
                     LLSSNRTVPNNPSINEYHSVIVFDPVVPTVDGRPAYESVWKYLLETDITPPPPPTDTT
                     APTVQVFSPSSGQIIESAPNA"
BASE COUNT          623 a          670 c          581 g          602 t
ORIGIN      
        1 cctgacgaac tgcccactac ggatccgcaa gccagcgcag ccaaacagga ttttttcaaa
       61 ctggtgagtt cctcaaatga agtcggtatt ttgtttaccg gccttccacc tgccggagat
      121 caactcagcc ggtttcaacg atttgggaaa attattcaat ttcaatcgct ggtgaattga
      181 ttgaggatgg aggacgtcag gtttggggct gaaaacttcg ggctgaagaa gtcgggctga
      241 aggtattgaa cccggttttt ttgccccgag cgtgcgagtc ttccgcctca agccctcagc
      301 cctggttttc accattcatc caaaaactgg ccgatccgcc ggaagtacac cacaatacag
      361 acggcaattg gcggataaac caacccggtt cggaccatat ccaggaacgt cactttttct
      421 tcgggcatca caaaagtgac tgatccaatg gcatttccaa tcacaatggc aatgacataa
      481 ccaagggcca aaaataccac ggctgaggct cgggtaaagg gctggtgcgg tgaatcgtcc
      541 gatgaagtaa ttgacaggat caggtctggt gaataggtgt agtgaatgag cacttcccgc
      601 aaaaacttta cgacctgcca gaaggcaaca caggccagca taaacaccag cacgtggccc
      661 acaagaccaa acggttcaac ggttgaaggt cgagcggcgg ccaccacaaa cgccgaagca
      721 aacaacgtcg ccacaactga aatggcatct gcccgcttac gcatttcccg ccgttcctct
      781 tcaataattt catcaacgac ccggttaatt cgtgaattat aagcggctgc ccgttgcgcc
      841 cgttcgcgat cagggtcaac ttccggttga tttgaacgtc ggcctccgcg acgcggaata
      901 tactcgcccc ggtcatattg ccttcgggat tgggggttgc ctaaaacctg ataggcttcg
      961 gccacttttt gaaacgttgc cttggcttct ggagcgtggc tgacatccgg atgatgttgg
     1021 cgtgccagcc gacgaaacgc agaacgaatc tcgcgttcgg ttgccgttcg cgagacaccc
     1081 aaaatctggt acaggtcatc catacgtgct ctagcgacct ccttatcagg aaaatatgga
     1141 ttttgggaag gtagaaattt gcggtttgcc agccgcatta taaaagcgaa aaggaaaata
     1201 gcgaagcgtg aatagcgatg aacaagaaga aaaaaccgct gtatgttgcg ttttttccaa
     1261 aacttatttc acctctccaa actcacattg gcggcaccac tttttgatcc aggggcaacc
     1321 ttgctattct ggatttcaaa ctggcactgg ctttcactca atcccaccaa ttttatgatt
     1381 ttgtgacgtc gctatttcac tctctccaca tatgaaacga acttatggca acctgctgtt
     1441 tttctggtca atcctcctgc tcatcgctgg ctggtcggtg cccgtacagg ctgaaaatca
     1501 attctggatt attggaccag ccattacgga tccggccatc aataccgcca ctgaatccca
     1561 ctatctctac ctcagccgat ccgttcaaca tcggaaccag ctcttccttt tctttccggg
     1621 tacgggtgca attccacgca actatctcaa gatctgcaat acagcggctg atatggggta
     1681 ccacgtgatc aatctccgct atccaaatgc tgaagccgtc aatcagcttt gtgggaccag
     1741 agcggatctg gattgttatg agcaggttcg agctgaagta attgatggca ctgaccgatc
     1801 accgctcgtt caagttaatc gagccaattc aattgaaaac cggctgatta agctcctcca
     1861 atatatggag cgaacctatc ctggagacgg ctggggtcaa tatcttgatg ctgacggtca
     1921 gccacgatgg gacctgattg ttaccagcgg tcactcacaa ggtggcggac acgctgccct
     1981 gctggcgaaa tatcaccggg tggcccgggt cgttatgttt gccgccatgg attttaattt
     2041 tttgcttggc aaacccgcca actggatcgg gaccaacaat gccacgccga catctgagta
     2101 ctatgcgttt tcccacgaac gcgatgagct ggtcagtttt tcgattcttt caaactcaat
     2161 ttatccagcc tatggaatga cggcctttgg tccagttacc agtgttgagg caacaccgct
     2221 gccgtttgag aacacccatc tcctgtcaag caatcgcaca gtcccaaaca acccttctat
     2281 caacgaatac catagcgtta ttgtctttga cccggtggtt ccaactgttg atgggcgacc
     2341 agcctatgaa tcggtttgga aatacctgtt ggaaactgat atcacaccac caccgccacc
     2401 gaccgacaca accgcaccaa ccgttcaggt ctttagcccg agttccgggc aaatcattga
     2461 atccgccccc aatgcg
//
LOCUS       DAOQDO010000837         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37414_length_2476_cov_2.748038, whole genome shotgun sequence.
ACCESSION   DAOQDO010000837 DAOQDO010000000
VERSION     DAOQDO010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37414_length_2476_cov_2.748038"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKZ53_13390"
     CDS             <1..319
                     /locus_tag="PKZ53_13390"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNJ41481.1"
                     /translation="YTGPKAALRPVHDKVMAEISKFGEFEISPKKTYVSLRRKKQFAM
                     VGPATKTQIEVGINSKTLTATDRLVEMPPNSMCNFKVRLSTVNEVDDALISWIKQAFE
                     SAG"
     gene            485..1204
                     /locus_tag="PKZ53_13395"
     CDS             485..1204
                     /locus_tag="PKZ53_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41482.1"
                     /translation="MSKKLHYIVLLILVLLIPETGYSQGKGKARTSKPSVDFSYLVGK
                     FSTQPDYVADMEVNAMNHIIHQTFAKKSGKIRNEFAPFEDSVTPVSNRARMYKVIVIA
                     SPDQPIIVLDPQAKTFTELQDAPMLQAFDISKYLQTLNKFARQVIVQNAGQAQTNGYQ
                     TTKYEMRFKRDPHGINFFFASDYQDLLIKVETSNPREKFSFSLTNVSLEVPDTLFEVP
                     KGYQEIDQAELKERFQALMAK"
     gene            1255..1632
                     /locus_tag="PKZ53_13400"
     CDS             1255..1632
                     /locus_tag="PKZ53_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HNJ41483.1"
                     /translation="MQKIISAMLIVVGIIHILPLTGVFGVQQLSNLYGLAFTEPNLVI
                     LMRHRAVLFGVLGVFCFYAAFRPAVQPLAFIAGFVSVVSFLWLAWSVGGYNPLVGRGV
                     LADLVALVCLLVATGLYWVAPKP"
     gene            1847..2467
                     /locus_tag="PKZ53_13405"
     CDS             1847..2467
                     /locus_tag="PKZ53_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNJ41484.1"
                     /translation="MIDWTAVATVFSVYCVGVISPGPNFVAVSHRAAATTRSQALALV
                     AGIVLVNLFWAASAILGIGIVFTAFPWVALLVKFTGAAYLIWFGWKLLTSTHPLSTSE
                     AEEAQKTNLTQAFVHGIATNIANPKSVAFYAAVFSSAAPAQVNFGTLLAMLSVVGVIA
                     FIWYGSVAVVLSHAPFAAAYRAQKRWIDRTCGVLIMALGIRQGLNK"
BASE COUNT          622 a          593 c          585 g          676 t
ORIGIN      
        1 ttacaccggt cccaaggcgg cgcttcgacc ggttcacgac aaggtaatgg cagaaatttc
       61 taaattcggc gagtttgaaa tttcaccgaa gaaaacctac gtcagcctgc gccgcaaaaa
      121 gcagttcgcc atggttgggc cagccaccaa aactcagatt gaagtcggca tcaattccaa
      181 aactctcacg gctacggatc ggctggtcga aatgccgccc aacagcatgt gcaacttcaa
      241 agtcaggctt tcgactgtta atgaagtgga tgatgccctg attagctgga tcaaacaggc
      301 gtttgagagc gcgggatgat agttgacttg tcaataattc catttgtact taccatctcc
      361 cgaccagtcc aaatttcaaa ggattacttt tcccaggtta aagtacaccc atccaacttt
      421 caggattctt gtttttccgt tgtgattgga tctggcactg cctgggaaaa ggaaagaaca
      481 gccaatgtca aaaaagctgc actacattgt tcttctgatt ctggtgcttt tgattccaga
      541 aaccggttac tcacaaggga aaggcaaagc cagaacctca aagccttccg tggatttttc
      601 atatctggta ggcaaatttt ctacccagcc ggattatgtg gcagatatgg aagtgaatgc
      661 gatgaaccac attatccatc aaacatttgc caaaaaatcc ggaaaaatca gaaatgagtt
      721 tgctccattc gaagactcag taacaccagt cagtaaccgg gcccggatgt ataaagtgat
      781 tgtcattgca tcacctgatc aaccgatcat tgtgcttgac ccgcaagcca aaacgttcac
      841 tgaactccag gatgcaccaa tgctgcaggc tttcgatatc agtaagtacc tgcaaacact
      901 caataaattt gccagacagg tcattgtgca aaatgcgggt caggcccaaa caaacggcta
      961 ccaaaccaca aaatatgaaa tgcgcttcaa acgtgacccg catggtataa atttcttctt
     1021 tgcaagcgat taccaggatt tgctgattaa agttgaaact tcaaatccac gggagaagtt
     1081 ttcattttcg ctcacgaatg tttccttaga ggttccagat actctctttg aagtaccaaa
     1141 aggatatcaa gaaattgacc aggctgagct taaagaaagg ttccaggctt taatggcaaa
     1201 gtaaccagtt tgggcaaaac cagatttgta cttatgcctt caaagagaga gatcatgcaa
     1261 aaaatcattt cagccatgct gattgtggtg ggtatcattc acattttgcc gctgacgggt
     1321 gtgtttggcg tgcaacagct ttcaaacctg tatggactgg catttaccga accaaatctg
     1381 gtgattctga tgcggcatcg ggccgtgtta tttggggtac ttggagtctt ttgcttttat
     1441 gccgcgtttc ggccagccgt ccagccactg gcgttcattg ctggttttgt cagcgtggtt
     1501 tcatttttat ggcttgcctg gtcggtcgga ggctacaacc cgctggttgg gcggggcgtc
     1561 ctggccgatc tggttgctct ggtctgtttg ctggtggcaa ctggcctgta ctgggttgcc
     1621 cccaaaccct gaaccctgag tctgaaatgg tgctgagacc aattagggtt cagggttcag
     1681 ggaaatacaa ttttttcaac agtttagctg tttgcaaata cgaggtcatc atcccataat
     1741 ggtgttattc cattttgcaa tgctcccatt caaagacagc caggtaattc aatcaaataa
     1801 actgagtgaa ttactgacta ctgactactg actactgact ggaattatga ttgattggac
     1861 cgccgttgct actgtttttt cagtgtattg cgtcggagtt atcagccctg gccccaactt
     1921 cgtcgccgtt tcacatcggg ccgctgccac cacacgctct caggcactgg cgctcgtggc
     1981 tggaattgtg ctggtcaacc tgttttgggc ggcttcggcc attctgggaa ttggcattgt
     2041 gtttacggct tttccctggg tcgccctgct ggttaaattc acaggtgccg cctatttgat
     2101 ctggtttggc tggaagttat tgacttcaac tcacccccta tcaacctcag aagctgagga
     2161 agctcaaaaa actaatctca cccaggcatt tgtccacgga atcgccacca acatcgccaa
     2221 tcccaaatca gtggcctttt atgcggcggt gttttcatcg gcggctccgg ctcaggtcaa
     2281 ttttggcacc ttgctggcca tgctgtctgt cgttggcgtg attgccttca tctggtacgg
     2341 atcagtggcc gttgtgctct cgcacgctcc ctttgcggct gcctatcgag cacaaaaacg
     2401 atggatagac cgcacgtgtg gtgtcctgat tatggcgctt ggcattcggc aaggattgaa
     2461 taaatagatg gaaaag
//
LOCUS       DAOQDO010000838         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37501_length_2472_cov_10.779479, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010000838 DAOQDO010000000
VERSION     DAOQDO010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37501_length_2472_cov_10.779479"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKZ53_13410"
     CDS             <1..747
                     /locus_tag="PKZ53_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41485.1"
                     /translation="DFNVLILADGSPYAYKEHFGEGGIQKLKAWCEEGGTLICLGNAS
                     AFAADKDVALTSARVVDGEDSKPDDASTDSKSDDAEDSKSKDAEKPEKSKNKKDSDKK
                     DSDAEKSDEKPKGEKKAKKPEPLPVPGAIMKAKVNRDHFLTFGYDQDVLPVLVNTGNF
                     FKLTDTGNNVLTFEGKNLYLSGYIWEKNTEQFIQDTAYVVEEQVGGGHVILFATEPNF
                     RFIWHASTKMFLNGLIYAPGLSGVSAGYRH"
     gene            948..1481
                     /locus_tag="PKZ53_13415"
     CDS             948..1481
                     /locus_tag="PKZ53_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41486.1"
                     /translation="MARTIWLMASMVGLILSFSLMTTGQEPVGRQVVKGYLVDKQTAL
                     DFLQYENPESKAKGYTKQQALSKEAQKKGYGILSGFTFLPFDGDGSKQAKKLFERTSK
                     QAGLYVEAEIVRREKVETFSRTKDTMDPLNNQYNLPPGTAIPSKESESGASIRLGGSD
                     RDFEVWSLKEVVAGEGK"
     gene            complement(1615..2361)
                     /gene="larB"
                     /locus_tag="PKZ53_13420"
     CDS             complement(1615..2361)
                     /gene="larB"
                     /locus_tag="PKZ53_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNJ41487.1"
                     /translation="MTRSELETLLREFQQGHHQLEDVVGRLEGWPSEDLGIATVDHAR
                     SARHGFPEVIFGASKSKDQVVTIAKSLLGRSPNVLITRTTFEVYEAIQHLSPAAQFHE
                     AAQAISIRRDQTMRGKGLILVVCAGTSDIPVCEEAVVTCETMGNRVERLFDVGVAGIH
                     RLFKAQDRLREARVVICVAGMEGALPSVVGGLVAVPVIAVPTSVGYGAAFGGLSALLG
                     MLNSCASNVTVVNIDNGFGAGYVASMINRL"
BASE COUNT          678 a          633 c          627 g          534 t
ORIGIN      
        1 gatttcaacg tcttgatact ggcggatggc agcccctatg cctacaaaga acactttggc
       61 gaaggcggaa tccaaaaact gaaagcctgg tgcgaagaag gcggaaccct catttgcctg
      121 ggaaatgcgt cagcctttgc cgctgacaag gacgtggccc tcaccagcgc ccgcgtcgtg
      181 gacggagaag attccaaacc cgacgacgcc agcaccgatt caaaatctga cgacgccgaa
      241 gattcaaaat ccaaagacgc tgagaagcct gaaaaatcaa aaaacaaaaa agattccgac
      301 aaaaaggact cagacgcgga aaaatctgac gaaaaaccaa aaggtgagaa aaaggcgaaa
      361 aaaccagagc cgcttccagt ccctggtgca attatgaaag ccaaagtgaa ccgtgatcac
      421 ttcctgactt ttggatatga ccaggatgtg ttgccggtgc tggtcaatac cggaaacttc
      481 tttaaattaa ccgacactgg aaacaacgtg ctgacctttg aaggaaaaaa cctgtacctg
      541 tcaggctaca tctgggagaa aaacaccgaa cagtttatcc aggatactgc ctacgtggtt
      601 gaagaacagg ttggtggtgg acacgtgatc ttgtttgcaa ccgaacccaa tttccgattc
      661 atctggcatg catcaaccaa aatgttcctc aacggattga tttatgcccc aggcttgagc
      721 ggcgtcagcg ccgggtaccg gcattgaaaa acagggctga agaagtcggg ctaagagctg
      781 agggctgaag acttcggact gaaaacagcg gggaattgag attggtttta cccactttct
      841 tcagccctga gcccaacttc ttcagcccga cttcttcagc cccgagccct gagcccagat
      901 accccaggcc cccaaaaaca aaatattcaa aggaaagtga gcacgctatg gcacgcacaa
      961 tatggctaat ggcctcaatg gtgggtctga tcttgtcgtt ttccctgatg acaacaggtc
     1021 aggaacctgt tggacgccag gtagtgaaag gctacctggt tgataaacaa accgccctgg
     1081 attttctgca atatgaaaat cctgaatcga aagcaaaggg ctataccaaa caacaggcgc
     1141 tctcaaaaga agcccaaaag aaaggctacg gcattctttc cggcttcacc tttcttccat
     1201 ttgatgggga tgggagcaaa caggcgaaaa agctctttga acgaaccagc aagcaggcgg
     1261 gtctctatgt tgaagctgaa atcgtcagac gcgagaaggt cgagacgttt tcacgaacga
     1321 aagacaccat ggatccactg aataatcagt acaatctgcc tccaggaaca gccattccgt
     1381 caaaagaatc tgagagcggt gccagtattc ggctcggcgg cagtgatcgg gattttgaag
     1441 tctggtcact caaggaagtt gttgcagggg aagggaaata gggttccggg ttccgggttc
     1501 cgggttccgg gttccgggtt ccgggttccg ggttccgggt tttcgaattt acgtcctttt
     1561 cgtccctgat gtcccgtttg tccttttttg ttttagtttc ccggaacccc aacctcacag
     1621 ccgattgatc atcgaagcca catacccggc gccaaacccg ttgtcaatat tgaccacagt
     1681 cacattggac gcacagctat tgagcatccc aagtaaggct gaaagcccac caaaggcggc
     1741 gccgtaccca acactggtcg gaacggcaat caccggaacc gctaccaatc cacccacaac
     1801 cgacggtaag gcgccttcca ttccagccac acaaatcacc acacgggctt cacgaagccg
     1861 gtcttgtgct ttgaaaagcc ggtgaattcc agccacgcca acatcaaata accgctcgac
     1921 tcgattcccc atcgtctcac acgtcaccac ggcttcttca cagactggaa tgtctgaggt
     1981 tccagcgcac accaccagaa tcaacccttt tccccgcatc gtttgatcac gtcgaatgct
     2041 gattgcctga gccgcctcgt gaaattgggc ggctggggaa aggtgctgga tagcctcgta
     2101 tacctcaaaa gttgttcggg taatcaggac atttgggctt cgaccaagga gtgatttcgc
     2161 aattgtcacc acctgatctt ttgatttcga agcgccaaaa atgacctccg gaaatccatg
     2221 gcgagccgag cgggcatggt caacagtggc gataccaaga tcctcagatg gccagccttc
     2281 caaccttccg acaacgtctt cgagttggtg atggccttgt tgaaattccc ggaggagtgt
     2341 ttcgagttca gagcgagtca ttgtattgag ggttcaggag ttagggttca ggagttaggg
     2401 tttagggttc agggtttagg gcttctgaag ggatgaggga tgagggatga gggatgaaaa
     2461 aaatcaccct gt
//
LOCUS       DAOQDO010000839         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37512_length_2472_cov_3.503103, whole genome shotgun sequence.
ACCESSION   DAOQDO010000839 DAOQDO010000000
VERSION     DAOQDO010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37512_length_2472_cov_3.503103"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKZ53_13425"
     CDS             <1..1179
                     /locus_tag="PKZ53_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41488.1"
                     /translation="RGQGTGGASQNPQSETQSPVSPPGTRNPEPGTLSFVDLGEAEPI
                     DQVVVTLRKMLSDRSKSVSTQVKPTAQVLDQLVMKPVRALVGNTKHLLISPDGALNLI
                     PFAALVDERGQFVVERYTLTYLTSGRDLLRLGVKFASHDAPFVIADPDYATGEGPHLL
                     GQIFPPLRRLIGTHMEGEKLKEIFPNAQLKLKGEANETILKQVNRPELIHIATHGYFL
                     KDAAEETSAKQGTRVLTRSEEPPIDPEKLRANNPLMRSWLFFAGANKGGSEDNDGTMT
                     ALEAAQLNLWGTKLVTLSACETAVGEAKTGDGVYGLRRALVLAGSEAQMMSLWSVSDQ
                     GTRELMVDYYTRLKNGEGRSEALRNTQLKLLKDPKRQHPFYWASFIQSGEWANLDGKR
                     "
     gene            complement(1255..1344)
                     /locus_tag="PKZ53_13430"
                     /pseudo
     CDS             complement(1255..1344)
                     /locus_tag="PKZ53_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1862..>2472
                     /locus_tag="PKZ53_13435"
     CDS             1862..>2472
                     /locus_tag="PKZ53_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNJ41489.1"
                     /translation="MPLRLQRVILVFVVGGLIMSQLSFASPMVAQQVPPTSRTSNPMS
                     TGIQKGFKAERNLSGGEQHTYPLTLKVGDYLKLVVEQRGIDVVVRLLGPDGNQLAKVD
                     SPNGTEGPEPLSLIAEQAGTYTLEVASFEKTARAGKYELNQEAVKPATEQDRARFEIE
                     ALVSKVEKLRQAGTYDEALTLATQALEKSEHVFGQEDLLVATSL"
BASE COUNT          653 a          621 c          669 g          529 t
ORIGIN      
        1 aggggacagg ggacaggggg ggcaagccaa aacccacaat ccgagaccca aagtccagtc
       61 tcgccgcccg gaacccggaa cccggaaccc ggaaccctgt cttttgtgga tttaggggaa
      121 gccgaaccga ttgatcaggt ggtggtgacg ttgcgcaaaa tgttgagtga cagatccaaa
      181 tcggtcagca ctcaggtcaa gccgacggcc caggtactgg atcagttggt gatgaagccg
      241 gtgcgcgccc tggttggaaa cactaaacac ctgctgattt cgccggatgg ggcattaaat
      301 ctgattccat ttgcggccct ggtggacgaa cgaggccagt ttgtggttga acgatacacc
      361 ttgacctatc tgaccagcgg acgggattta ctgcggctgg gagtgaagtt cgccagtcac
      421 gatgcgccgt tcgtgatcgc cgacccggat tatgccactg gcgaggggcc acacctgttg
      481 ggacaaattt ttccaccttt acgacggttg atcgggacac atatggaagg ggaaaagttg
      541 aaagaaatct ttcccaatgc gcaattgaag ttgaaaggag aggccaacga aaccattttg
      601 aaacaggtga accgacccga actgatccac attgcgaccc atggatactt tctgaaagac
      661 gctgccgaag agacttcagc caaacaggga acccgggtgt tgaccagatc tgaggagccg
      721 ccaatcgatc ctgagaaact gagggcaaat aacccgttaa tgcgctcgtg gctgtttttt
      781 gcgggagcga acaaaggtgg ttcggaagac aatgatggca caatgacggc gttggaagcg
      841 gcgcaactca acctgtgggg cacaaagctc gtcacgctct cagcctgtga aactgcggtt
      901 ggcgaagcca agactggcga tggtgtgtat ggattgcgcc gggcactcgt cctggccgga
      961 agcgaagcgc agatgatgag tttgtggtcg gtgtcggatc aaggaacgcg ggaattgatg
     1021 gtggactact acacccggct caaaaacggt gaaggccgat ctgaagccct gcgcaacacc
     1081 caactcaaac tgctcaaaga ccccaaacgc cagcatccgt tttactgggc gtcgtttatt
     1141 caatcaggcg aatgggcgaa tctggatggg aaacgataaa atggagaatg atttaactcg
     1201 aactcaaagt ataatttgcg ctcgaaaaag cggtaatcga gcagcggtag catcaggctg
     1261 gctgacgtag cacgcttcgg cagccttccc aaagtgcttg aggtcaacca cggccagaaa
     1321 ataggtcaaa tcacggatgt tcataccttg gtgcttggtg cttgggactt ggtgcttggg
     1381 acttggtgct tgggacctgg cagagaactt atatcatttt caaaccgtgg ttgggcactg
     1441 gcatttagct caatgattga aaaaccggta tttccccaca accctgaacc ctgaaccctg
     1501 aactctgaac cctaaaccct gaaccctgaa ccctatgaat ttcaatctcc tgtagctcga
     1561 ttgagagtca gtcccctgtt cgatcaggga tttgacagtt tccagatgac cagcccttgc
     1621 ggaatagtgt catgacattg ctccaaactg gtcgaatgcc gggtaaaaaa cgcagtcatg
     1681 acggtactga tatgatattt tggttctttc ctgaatcata atcacaaccg accacctgtt
     1741 ttggccaaaa cctgagtcaa aaagcctggt atgacctggg ttacgcacgt cagatcattg
     1801 ttcctgaccg cgcaacagct acagattttt catcaatcaa cccaaacaag gagaatcccc
     1861 gatgcccctt cggttacaac gcgtcattct ggtgttcgtg gttggtggct tgatcatgtc
     1921 ccaactttct ttcgcatcgc ccatggttgc ccagcaggtt ccgccaacgt cacggacctc
     1981 aaacccaatg tcaacgggaa ttcaaaaagg gtttaaagcc gaacgtaact tatcaggtgg
     2041 tgaacaacac acctacccgc tcaccctcaa ggtcggtgat tatctgaaac ttgtggtgga
     2101 gcaacgcggg attgatgttg tggtgcgatt gctcggtcca gatggaaacc aattggccaa
     2161 agtggatagt cccaacggca cagaaggccc agagccgttg tccctgattg cagagcaggc
     2221 aggcacctat acactcgaag tcgcctcatt tgaaaaaaca gccagagccg gaaaatatga
     2281 attgaaccag gaagcggtga aaccggccac cgagcaggat cgagccaggt ttgaaattga
     2341 ggcactcgtg tcgaaagttg aaaaactccg ccaggcaggt acatacgatg aagcattgac
     2401 tctggcaacc caggcgctgg aaaagagcga acacgtcttt ggtcaagagg acctgcttgt
     2461 cgccaccagc ct
//
LOCUS       DAOQDO010000840         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37521_length_2472_cov_2.664046, whole genome shotgun sequence.
ACCESSION   DAOQDO010000840 DAOQDO010000000
VERSION     DAOQDO010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37521_length_2472_cov_2.664046"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..446)
                     /locus_tag="PKZ53_13440"
     CDS             complement(96..446)
                     /locus_tag="PKZ53_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41490.1"
                     /translation="MALDFGIVSRVEKHLESGLIRGFFTNEKFRFQRSQLIDFPLPFQ
                     ADLFSSYPKETHFFFEPDENRSVKKVSRAWAHINQIPRAMVEGFFRTALDRYQKEAMV
                     SPIIGNALLQRLVQ"
     gene            complement(496..939)
                     /locus_tag="PKZ53_13445"
     CDS             complement(496..939)
                     /locus_tag="PKZ53_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41491.1"
                     /translation="MMIFREATHADIPAMSEIRLLVKENVLSNPGRITRQMYEDYLGP
                     LGKGWVCELDGTVVGFSYAACEDHSIWALFIHPDHEGNGIGSRLLKLATDWLFERGAS
                     RVILSTEVNTRADRFYAAQGWHRGDIKGEFPEVVYTLERPAQVTR"
     gene            complement(960..1460)
                     /locus_tag="PKZ53_13450"
     CDS             complement(960..1460)
                     /locus_tag="PKZ53_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41492.1"
                     /translation="MRNPVESLLRLPGVIDVAQLLVLRVTSQIFPARAYRLTRQPQTT
                     DPDLKNAEGYIQGFRLTETWALQPSVAQSLWKTIFQLKLYGNRSAFGMRCFRPGTAIE
                     FGQGAGSIQVVICLGCHWVYFHRGNWRLALALTPRGVTELRDALSNRLLRRVEMKAAE
                     FSQVKA"
     gene            complement(1457..1957)
                     /locus_tag="PKZ53_13455"
     CDS             complement(1457..1957)
                     /locus_tag="PKZ53_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41493.1"
                     /translation="MRLIRLQKLDVLIVLIFFGVLVSFSSQTQASQQSSDSKQNQSTQ
                     KVTLKFLRLEKVQDTYNKEIVAFFKLANLTSHPFRYETEAAQPVFPVHERLYRSWFGW
                     KNHLLYIDDSGWGEHELQPGESIEVQVRLESQDYNRALRIAIYGRNNNQVIRVVSQTF
                     EYRQHK"
     gene            complement(2013..>2472)
                     /locus_tag="PKZ53_13460"
     CDS             complement(2013..>2472)
                     /locus_tag="PKZ53_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41494.1"
                     /translation="VRYCTRCGSNFEVVTRPAPVLGEPTMLVGISQVSWLLACGALLG
                     VSIFFLVFLIFDFIRDRFHPGRPEQLVLLLMTCLGLAVGMVGTACYLASKLVLSFAKA
                     GQASLLKSPPINPPEIEAPRQTTGLDEPAWFSGKVPPRSVVEPVTNRTQG"
BASE COUNT          645 a          634 c          608 g          585 t
ORIGIN      
        1 tattttcgtt ccaggaagcg acaggtgtaa tcgggacaga ccgaaaaata tcggcatgcc
       61 agaagttctc gaagtttatt ttcagtgagt tcttctcact gcaccaaccg ttgtaacaac
      121 gcattgccga taattgggct caccatggct tccttctggt accgatccag tgccgttcgg
      181 aaaaatcctt ccaccatggc tctcggaatt tgattgatgt gcgcccaggc cctggaaacc
      241 tttttcacgg accggttttc gtccggctca aaaaagaaat gggtttcttt cggataactc
      301 gaaaacaaat cagcctggaa gggcagtgga aaatcaatga gttgagaccg ctgaaacctg
      361 aatttttcat tggtgaaaaa accgcgaatc aagccggact caagatgttt ctccaccctg
      421 gaaacaattc caaaatcaag cgccatcgtg cctccttttt ccgccacgtc gctcaccaat
      481 caactggcca ccctgtcacc ttgtcacctg cgccgggcgt tccagggtgt agaccacttc
      541 gggaaattca cctttgatgt caccccggtg ccagccctgg gcggcataaa atcggtcggc
      601 acgggtattg acttcggtgc ttaaaataac ccgcgatgcg ccacgttcaa acagccagtc
      661 agttgcaagt ttcaagagcc gtgacccgat gccgttgcct tcatggtccg ggtgaataaa
      721 caaggcccag atggaatggt cttcgcaggc agcgtaggaa aacccgacca ccgttccatc
      781 aagttcgcac acccagccct ttccaagcgg gccaaggtaa tcttcataca tttgtcgagt
      841 aatccggcct ggattggaaa ggacattttc tttcaccagt aagcgaattt ctgacatcgc
      901 cggaatgtca gcgtgtgtag cttcccgaaa aatcatcatc ataccgcctt ttatcaagtt
      961 caagctttca cttgtgaaaa ttctgccgct ttcatctcaa ctcgccggag caaacgattg
     1021 gataaggcat ctcgcaattc ggtaacaccc cgaggagtta aagccagtgc caatcgccag
     1081 ttgcctctgt gaaaataaac ccagtggcac ccaaggcaaa tcacaacttg tattgaacca
     1141 gctccctgcc caaactcgat ggccgtgcct ggacggaaac agcgcattcc gaaggcgcta
     1201 cgattcccat agagcttcaa ctgaaaaatt gttttccaca gtgattgcgc cacggatggt
     1261 tgaagcgccc aggtttcagt caatcgaaac ccctgaatat acccttccgc gtttttcaga
     1321 tccggatcgg tcgtttgtgg ctggcgggtt aaacggtagg cccgagccgg gaaaatttgt
     1381 gaggtgacac gcagtaccag gagttgagcg acatcaatca cgccgggcag tctcaatagt
     1441 gattcaaccg gatttctcat ttgtgctgcc gatattcaaa ggtctggctg acaacccgaa
     1501 taacctgatt gttgttacga ccataaatgg caatcctgag tgcccggtta tagtcttgag
     1561 attcaagtct gacctggact tcaattgatt cccctggttg gagttcatgt tctccccagc
     1621 ctgagtcgtc aatataaagc aggtgatttt tccaaccaaa ccaggaacga tagagccttt
     1681 catgaactgg gaaaactggc tgggcggcct ccgtttcata ccggaatggg tggctggtca
     1741 aatttgcaag tttaaagaag gcaacaatct ctttgttgta tgtgtcctgg actttttcaa
     1801 gcctcaaaaa ttttaatgta accttttgag tagattgatt ttgtttgcta tcggatgatt
     1861 gttgactggc ctgggtctga gaagaaaagg aaacgagtac tccgaaaaag atgagaacaa
     1921 tcaaaacatc gagcttctgc aggcgaatga gacgcatgat gtggtctcca cagcggaaag
     1981 tctcaggaaa atatggtcag agaggattta ttttatccct gggtgcgatt ggtgaccggc
     2041 tcgaccacgc ttcgcggagg cactttcccg gaaaaccagg ctggctcgtc aaggccagtg
     2101 gtctggcggg gagcttcgat ttcgggggga ttgatgggtg gtgacttcaa taacgacgcc
     2161 tgcccggctt tggcgaaaga caggaccagt ttagatgcca gatagcaggc ggttccgacc
     2221 attcccaccg ccagacccag gcaggtcatc agcaacagca cgagttgttc gggtcttcct
     2281 ggatgaaacc ggtcacggat aaaatcaaaa atcagaaaca caaggaaaaa gatcgaaacc
     2341 cccagcaagg caccacaggc cagaagccag cttacctgtg agatcccaac cagcatggtg
     2401 ggctcaccca ggacaggtgc gggacgggtc accacttcaa aatttgaccc gcaacgggtg
     2461 caatatcgga cc
//
LOCUS       DAOQDO010000841         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37522_length_2472_cov_2.647083, whole genome shotgun sequence.
ACCESSION   DAOQDO010000841 DAOQDO010000000
VERSION     DAOQDO010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37522_length_2472_cov_2.647083"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKZ53_13465"
     CDS             complement(<1..287)
                     /locus_tag="PKZ53_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41495.1"
                     /translation="MRLTENLSSRAKRLTGFLMVLTLLGFAAMPFLPAPSSLASSGKG
                     LINKLIAPKKQEPAQKQESATKKEATSEEKTLTYDEIQKIKEDRVKEENRLA"
     gene            complement(697..1104)
                     /locus_tag="PKZ53_13470"
     CDS             complement(697..1104)
                     /locus_tag="PKZ53_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41496.1"
                     /translation="MKSSTAFLATLSKLNEVSDMVRAFVTRVMLVVVMVGLTVVPAMA
                     RNVSKKMTLPRACVIQGQTINAGTYTAKFVDDQNSELVILDGKKELARLPYKLTSLEK
                     SAASDVVVFNNQNGTRSLSRIEFKGLNAALQFE"
     gene            1603..2274
                     /locus_tag="PKZ53_13475"
     CDS             1603..2274
                     /locus_tag="PKZ53_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ41497.1"
                     /translation="MNSHFPEPAPLTSAEIGNRPKQERSRRTREKIIEAALKLFDERG
                     FEKTTSNDIALAAGVSIGSFYVYFTDKRQVLLLVLERLLSDRIDAVFSNFQADDHTLP
                     SLRKQIRQAVARAFYNKCLTPGLTRVIQELAGKDEEIAQLRKKFVEKSIHQLRDILAT
                     CEHQGLTNHVDVNIASRVINHAVDAVAYEYMLSGNRSEEEKHVLIEELTDMIFRYVFK
                     PIPVE"
     gene            2292..>2472
                     /locus_tag="PKZ53_13480"
     CDS             2292..>2472
                     /locus_tag="PKZ53_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41498.1"
                     /translation="MKNEELRIENEELRMTSILPKIPTCSLVLVPDETKIKGHKGHKG
                     RKGPVRSRFYRSDEEN"
BASE COUNT          682 a          574 c          583 g          633 t
ORIGIN      
        1 gctaagcgat tttcttcttt gactcggtct tctttgatct tctgaatctc atcataagtc
       61 agagtttttt cctctgatgt ggcttctttc ttcgtagccg actcttgttt ttgagccggc
      121 tcctgtttct ttggcgcaat gagtttattg atcaatcctt tgccgctact ggcaagggat
      181 gaaggtgcgg gcagaaacgg catggcggca aagccaagca atgtcagcac catcaggaat
      241 ccagtcaggc gtttggcgcg gctggatagg ttttctgtaa gacgcataat tctcctcccg
      301 gggtcgaact gcatttttct ggcacaaatg tattggtgga ttgaaaattc cctggcccaa
      361 acactggact cctcgaaacg gcgggtctgt gatctgcagg tgtgtggaaa aatgcacaac
      421 ttctggtgac cttcgctggc ggggagcgaa gacgaaaaat tcgattgaaa agtgacgtgt
      481 ggttgaaaaa tcgagttcgg aatgaacaca gagtaagggt ttcagatttg ttacattatt
      541 ataaccatac tgtcaaccta acaatctgac ttcagaatga tttcgatgaa aaaagaaagg
      601 gacgttttcc tggtggaaaa cgtcccgtaa agaatccaaa aaaatgtagc gctacatgtt
      661 taaggaggag tggctacgac tcagaacatt cagtgcttac tcaaactgca aggccgcatt
      721 cagcccttta aattcaattc ggctcaggct gcgggtacca ttctggttat tgaagacgac
      781 gacatcgctg gcggctgatt tctcaagcga agtgagttta tagggcaggc gggccagttc
      841 ctttttgccg tccagaatca ccagttcgct attttggtca tcaacaaact tcgcggtgta
      901 ggttcccgca ttgatggttt ggccttgaat cacacaggcg cggggcaagg tcatcttctt
      961 gctcacattg cgggccatcg ccggcacaac ggtcaatccc accatcacca cgacaagcat
     1021 cacacgggtc acaaacgcac gcaccatatc actcacctca ttaagtttgg aaagagttgc
     1081 cagaaaagct gtggatgatt tcaacgtaaa ttcatccaaa atgaacttga acttttattc
     1141 agttttgata aacataaaat atgagctcaa gttcattttg tcaagaaaaa aaaccggaga
     1201 taaaagctgg ggctgaagac actgggctca gggctagggg ctgagggctg aagaactcgg
     1261 gttgaggaac tggttttatt tcatccctca tcccttcgat tgccctgagc ttgcgagtct
     1321 tcagcccgaa gtcttcagcc ccaagccctg agccctggtt ttcagcccgc tgccttctga
     1381 tgcacagggt caacgcgtca tgacagatcg aaaattgtct agtaccattt gattatccac
     1441 gggttaccga tatcaccttc atttgtcacc tgattctctc cgaaagattt gtgtctgaaa
     1501 cgtccaggca gcttgttacc caatgacagg catcaccacc ttgtcggctt tttttgccca
     1561 gaagatcaaa aagaaagtat tcgccaaaat tgttgttgtt ttatgaattc acacttccct
     1621 gaacctgccc cactcacctc agccgaaatt ggaaatcgcc cgaaacagga gcgtagccgc
     1681 cgaactcggg aaaaaatcat cgaagccgcc ctgaaactct ttgatgaacg tggctttgaa
     1741 aaaaccacat ccaatgacat cgcactggca gccggggtca gtatcggctc gttttatgtg
     1801 tatttcaccg ataagcggca ggtcttgctg ctggtcctgg agcgactgtt aagtgaccgc
     1861 attgatgctg tattcagcaa ttttcaagcg gatgaccaca cgctgccaag cttgcgcaaa
     1921 cagattcggc aagccgtggc acgtgctttc tacaacaaat gcctgacacc aggactcaca
     1981 cgtgtcatcc aggagttagc tggtaaagac gaagaaatcg cccagttacg gaaaaaattt
     2041 gttgaaaaat caattcatca actgagggat attttggcga cctgcgaaca tcaaggcttg
     2101 accaaccacg tggatgtcaa tattgcctca cgcgtgatta accacgccgt tgatgccgtg
     2161 gcatatgaat acatgctctc aggcaaccgc tctgaagaag aaaaacacgt cttgatcgaa
     2221 gaactcactg acatgatctt tcgctatgtg ttcaagccca ttccggttga ataaagaatg
     2281 aagaatgaag aatgaagaat gaagaattga gaattgagaa tgaagaattg agaatgacct
     2341 caattttgcc caaaataccg acctgttccc tggttttggt acctgatgag accaaaatca
     2401 aaggacacaa aggacacaaa ggacggaaag gaccggttcg aagtcggttt tatcggagtg
     2461 acgaggaaaa tt
//
LOCUS       DAOQDO010000842         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37582_length_2470_cov_2.660870, whole genome shotgun sequence.
ACCESSION   DAOQDO010000842 DAOQDO010000000
VERSION     DAOQDO010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37582_length_2470_cov_2.660870"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>2470
                     /locus_tag="PKZ53_13485"
     CDS             36..>2470
                     /locus_tag="PKZ53_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNJ41499.1"
                     /translation="MEGFFDVCARLYMPHLVSALRSIDFLYSDTKRSAQALDYEDLQL
                     FARDLLVNAPDVRRRAAARYRYFLVDEFQDTNRLQREIIGLLALDTTSRSAEGQSLFL
                     VGDRKQSIYNFRGAEVEVFEEAIQEIQRRRGEHIALDVNFRSDSRLIRFFNGFFSRLM
                     RLEAGEDRAHFLSLGFVEHEPGIPFRAQLDDGPAVELLYYVPEPETDEEKKEADPDDE
                     SLREIEARRLAARVKQIVENGEELVRTKDGGKRGAIYKDFAVLLGAFTQVKAYEQAFQ
                     RAGVPYYVVAGRGFYSRPEVLDLLTLLEFLDNRTDEISLAAILRSPLFGISDETLYSL
                     RWAEELTPGVPPIVPPNESFMPPTEPAVPTVIAIKRKAGRPMFAALLNHANLANITGE
                     QHALLAAASETLQDLMYVRNRLPLSELIERAIRRTQYDVMCAAAEDGPQRLSNLDKLI
                     GQARGFEQHEARLLRDFVEYIRNFRRLEGREAEAQLELGLNAVAILTVHKSKGLEFPI
                     VVIPDLNHKRRPYRDVFLFDRERGLAFQVPDQRGGKVATALYAEIQERLELREQFESM
                     RQLYVGMTRAEDYLILSAASKQMKPTEKLIREQDSWLRWLCTVLGTHDMPGESVVECG
                     ETQALVRGREIELSVEPIVVESATPDAGSKRTPMRTPQQQRERQRNLAALQSQLEQQM
                     EPVPASEETQSALHYRYSVTQLVNYLNCPRQYYFARFLQMPDMELKAGSGRADDAEAR
                     EGRARLSPTLRGTIVHRFCELFREGDDLFDTLRQALDDIRQTRVDEWADELASIDDEE
                     ALKDLWPLAKNYV"
BASE COUNT          554 a          664 c          702 g          550 t
ORIGIN      
        1 tctttggaca caaagacttt ggcgcaggga aactgatgga agggtttttt gacgtttgtg
       61 cccggctcta tatgccgcat ttggtttcgg cgcttcggtc aattgatttt ctctatagcg
      121 acaccaagcg cagtgcccag gcgcttgatt atgaagacct ccagcttttt gcccgtgatt
      181 tgcttgtcaa cgcacccgat gtccgccggc gagcagcagc ccggtatcgc tattttctgg
      241 tggacgaatt tcaggacacc aaccggctgc aacgcgaaat cattggttta ctggcccttg
      301 ataccacttc gcgaagcgct gaggggcaga gcctgtttct ggtcggagac cgcaaacagt
      361 ccatttacaa ctttcgcggc gctgaagtcg aagtttttga agaagccatc caggaaattc
      421 agcgtcgtcg aggcgaacat attgccctcg atgtcaattt tcggagcgat agccggttga
      481 ttcggttttt caacgggttt ttctcgcgcc tgatgcggct tgaagctggc gaagaccgtg
      541 cccattttct gtcgcttggg tttgtcgagc acgaaccggg gattccgttt cgggcccagt
      601 tggatgacgg tccggcagtg gaactcctct actatgtacc tgagccagaa accgatgaag
      661 aaaagaaaga agccgatccg gatgacgaat cgcttcgcga aattgaagcc cgccggctgg
      721 cggctcgtgt caaacagatc gttgaaaacg gcgaagaact ggtccgcacc aaagatggcg
      781 gcaaacgtgg agcgatttac aaggattttg ccgttttgct tggcgccttc actcaggtaa
      841 aagcctatga acaggcattc cagcgcgccg gagtgcccta ttacgtcgtg gctggtcgcg
      901 gattttattc gcgcccggaa gtgctggatt tattgacctt gctggaattt ctcgacaatc
      961 gaaccgatga aatcagcctg gcggccattt tgcggtcacc gctctttggc atttcggatg
     1021 agacgctcta ttcacttcgc tgggctgaag aactgacccc aggcgtgccg ccgattgtgc
     1081 cgccaaacga aagttttatg ccgccgaccg aaccggctgt cccaacggtg atcgccatca
     1141 agcgcaaagc cgggcgtcca atgtttgccg cactcctcaa tcacgccaac cttgccaaca
     1201 ttactggtga gcaacatgcc ttgctggcgg cagcttcgga aacgctccag gacctgatgt
     1261 atgtccgcaa ccgactgccg ctttcggaat tgatcgaacg tgccatccgt cgcacgcaat
     1321 acgacgtgat gtgtgccgcc gccgaagatg ggccacaacg gttgtcaaac ctcgacaagt
     1381 tgattggcca ggcccgtggg tttgaacagc acgaggcgcg gttgctacgt gattttgtcg
     1441 aatacattcg caatttccga cgactcgaag gccgcgaagc cgaagcccag cttgaacttg
     1501 gcctcaacgc ggtggcgatt ctcacggttc acaaatcgaa ggggctggaa tttcccatcg
     1561 ttgtgattcc agacctgaac cacaaacgcc ggccctatcg cgatgtgttt ttgtttgacc
     1621 gtgaacgtgg gctggcgttt caggtgcctg accagcgtgg agggaaggtc gcgacggcac
     1681 tctatgcgga aattcaggag cgactggaac tccgtgaaca attcgagtct atgcgtcagt
     1741 tgtatgtcgg gatgacccgc gccgaggatt acctgattct gtcagccgct tcaaagcaga
     1801 tgaaaccaac cgaaaagctg attcgtgaac aggatagctg gcttcgctgg ttgtgtacgg
     1861 tgctgggaac acacgatatg ccgggggaat cagtagttga atgtggagaa acccaggcac
     1921 tggttcgagg tcgtgaaatt gaactcagcg tcgaaccgat tgtggttgaa tcagccactc
     1981 cggatgcggg cagcaaacgc acgcccatgc gtacgccaca gcaacagcgt gaacgtcagc
     2041 gcaatctggc cgcgctccaa tcccagcttg aacagcaaat ggagccggtg ccagcgtcgg
     2101 aagagacaca atcggcactc cattaccgct acagcgtgac acagttggtc aactacctga
     2161 actgcccgcg ccagtattat ttcgcccggt ttttgcagat gccggatatg gaactcaaag
     2221 ccgggagcgg acgggccgat gatgccgaag cgcgcgaagg ccgcgcccgg ttgagtccga
     2281 cgctgcgtgg aacgattgtg catcggtttt gcgaactgtt ccgcgaaggc gacgacctct
     2341 ttgacacgct tcggcaggcg ttggatgaca tccgacagac ccgcgtagac gaatgggccg
     2401 acgaactggc ctcgattgac gacgaagaag cactcaaaga cctctggcca ctggcgaaaa
     2461 attacgttta
//
LOCUS       DAOQDO010000843         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37650_length_2467_cov_3.046849, whole genome shotgun sequence.
ACCESSION   DAOQDO010000843 DAOQDO010000000
VERSION     DAOQDO010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37650_length_2467_cov_3.046849"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PKZ53_13490"
     CDS             <1..1000
                     /locus_tag="PKZ53_13490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNJ41500.1"
                     /translation="KNTGSLATLIMVRPAEEIGATFVHHANPLPPHLRISVVIPTHNR
                     PAFLAEALASVMAQTMPPFEVIVVENGSTPSTQAELQKLVVQYPEITLHHLSENLGCS
                     AARNLGLDVATGDLIFFFDDDDLVAPTLFETALHHFCQHPDSDALVVSYQNLIHHAEL
                     DSPYFPTSLFDYRTKGPELERNAFRVFLRSCPPIHSVVIRRQAIGDLRFVEHLEVGED
                     WLFWLQLARQNCHFAYCPQPLVYYRRHAGSKSHHQRYFFSELEQFLTYLQTTSNLLES
                     RHDRFFIESKLFFTCWSLRNPRFFWKGLRILMYPEWIVHYSLWLIATWMRRLTHGK"
     gene            990..2234
                     /locus_tag="PKZ53_13495"
     CDS             990..2234
                     /locus_tag="PKZ53_13495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ41501.1"
                     /translation="MESNRPNLLFLSLVEPSLHSNGPAQRAYFTLQAFSRFYDIHLVI
                     LEVGFYEKPRSTGPDPLPCQSMTNIQVPMGLDCGYFTRRGIFRLSPQLFFSLYQQPSS
                     ERFFSEMTAVQLATKLAGTPFDILHGFRLYTLPWAKIIATRLKIPLLQIDLDDLESHT
                     RKRLADLYRSRHLRHLAAQFEADAQEFLKVEHTLDRSCRRVFVCSELDRHHLQRQLST
                     LDVVVLPNVVRLPEVQPLQRKHRSFTFLFVGTLGYFPNQDALEYFCATSWPMIVSRAQ
                     GAVELCIVGDGLPSHIKTWLARQPGVVLSGWVANLEDVYARADAVVIPLRAGGGTRIK
                     LLEAMAYGVPVVSTSLGAEGIEVQPGTHAILADTPEAFAASCLRLLEDTALRTHLAQS
                     GKALVNALYTEDALIRQIGALG"
BASE COUNT          533 a          673 c          602 g          659 t
ORIGIN      
        1 aaagaacact ggttccctgg ccactctcat tatggttcga ccggctgaag aaattggcgc
       61 cacatttgtc caccatgcca acccattgcc gccccacctg cggatttcag ttgttattcc
      121 aacccataat cgccctgctt ttttggccga agccctcgcc tcagtgatgg ctcagacgat
      181 gccacctttc gaagttattg tggttgaaaa tggctcaacc ccatcaaccc aggcagaact
      241 tcaaaagctc gtggttcaat atccagagat taccctgcat cacctttctg aaaatctggg
      301 ctgttccgcc gcaagaaacc tgggtctcga cgtggccact ggtgacctga tcttcttctt
      361 cgatgatgac gatctggtgg cgccaacttt gtttgaaaca gcgctccacc atttttgtca
      421 gcatcctgac tctgatgcgc tggtggtttc atatcagaat ctgatccatc acgctgaatt
      481 ggattcaccg tattttccaa cctcattgtt tgattaccgg acgaaggggc cggaacttga
      541 acgcaatgcg tttcgggtct ttctacggtc gtgcccgcca atccactcgg ttgtgattcg
      601 acggcaggca atcggagacc ttcgttttgt cgagcatctg gaagtcggtg aagactggtt
      661 gttttggctt cagcttgcca ggcagaattg tcattttgcc tattgcccgc agccgctggt
      721 gtattatcgg cggcatgccg gctcaaaaag ccaccatcag cgctactttt tttccgagtt
      781 ggagcaattt ttgacttatc tccaaaccac gagcaacctg ctggaaagtc gccatgatcg
      841 tttttttatt gaatcaaagc tttttttcac ctgctggtcg ttgcgcaacc cacgattttt
      901 ttggaaggga ctccggatcc tgatgtatcc agagtggatc gttcactata gtctgtggtt
      961 gattgcaacc tggatgcggc gcctcacgca tggaaagtaa ccgtccaaac cttttatttt
     1021 tgtctctggt tgagccttcc ttacactcaa acggcccggc gcaacgggcc tattttaccc
     1081 ttcaggcatt ttcccgattt tacgacattc atctggtcat tctggaggtt ggtttttatg
     1141 aaaaaccacg ctcaaccggg ccggacccac tcccctgtca atccatgaca aatatccagg
     1201 tgccgatggg gcttgattgt ggatatttca cccgcagagg gatctttcgc cttagcccac
     1261 agctcttttt ttccctgtac cagcaacctt cgagcgagcg attcttctca gaaatgacgg
     1321 cagtccagtt ggccaccaaa cttgctggca ctccgtttga tatccttcat ggattccggt
     1381 tgtatacatt gccctgggca aagatcattg ccaccaggtt gaaaattccc ttgcttcaaa
     1441 ttgatttgga cgatctggag tcacacacca gaaagcgtct ggctgatctg tatcgaagcc
     1501 gacacctgcg acacctggct gcacagtttg aggccgatgc ccaggaattt ttgaaggtag
     1561 agcacaccct tgatcgctct tgccgccggg tctttgtgtg ttctgaactc gaccggcacc
     1621 acctgcaacg ccagctctca accctggacg tcgtggtgct cccaaatgtt gttcgcttgc
     1681 ccgaggttca acctctacag aggaaacacc ggtcatttac ctttttgttt gtcgggacat
     1741 tggggtattt cccgaatcag gatgccctgg aatacttttg cgccaccagt tggccaatga
     1801 ttgtatctcg tgcccagggt gcggttgaat tgtgcattgt tggtgatggt ttgccctccc
     1861 acatcaaaac ctggctggcc agacaacccg gcgtcgtgtt gagcggctgg gtggcgaatc
     1921 tggaggacgt ctacgccaga gctgatgcgg tggtaatccc acttcgggct ggcggcggaa
     1981 cccgcattaa actcctggaa gctatggctt atggcgttcc ggttgtttca acgagccttg
     2041 gtgctgaagg cattgaggtt cagcctggaa ctcacgccat tctggccgat accccagaag
     2101 cgtttgccgc cagttgtctc cggttgcttg aggataccgc cttacgcacc catcttgctc
     2161 agagtgggaa agcactggtg aatgccttat atacagaaga cgccctgatc cgacagattg
     2221 gtgcattggg ctgaaaagac cagggttgat tgcctcaacg tcttcagccc caagccctca
     2281 gccctggtct ttccagctat tcatcccgaa gcgtccgcat tggatcaatc tgaatagcac
     2341 gttgaactgg aaggtaacag gcagagagcg cgaccagtcc caaaaccacc acgacactgc
     2401 caatcgtcat cggattggtt gggctgattt caaaaagcag gctggtgaga aacctggaaa
     2461 aaccaaa
//
LOCUS       DAOQDO010000844         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37676_length_2466_cov_3.788055, whole genome shotgun sequence.
ACCESSION   DAOQDO010000844 DAOQDO010000000
VERSION     DAOQDO010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37676_length_2466_cov_3.788055"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /gene="bamA"
                     /locus_tag="PKZ53_13500"
     CDS             complement(<1..1798)
                     /gene="bamA"
                     /locus_tag="PKZ53_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNJ41502.1"
                     /translation="MALLSLGVVTPLQVQAAASHLSSTLRSPQDESAQQRIVEDVEFR
                     GNRRIPTDTIRLRVSTKPGDIYNRDQVERDYQAIVSEGFFDEFESRVTVEEGFRGGVI
                     IVFVLRERPIIRDIQYEGLKSVQLSDVLTRYREKGISLTKDSQFDPVKVRRAEDELER
                     MLSEKGRPEADVKADIEDISATTVVIVFNVDEGPRVRVTKIDFEGNKVFSDRQLKGAM
                     KLTKQSSLITIFTSKDVYDPGKFENDMLLVRQFLLDRGYLRPVIGEAKVEEAGMVRSR
                     WIFFGKPSRALKITIPIDEGILYRFGDLSVEGSTIFTPEQVLAVSGMKKGDIASGKTI
                     REGVYERLKKAYGSQGYIQAETSLNQDFKPVAPDAKEGVVDFTLNVTEGSVFTIRRIE
                     FLGNSVTRDTVLRRELLVDEGTPYNQELWEYSILRLNQLGFFEEIKKEDAQIQTDERN
                     KEVDINLRVKEKGRQQIQFTGGVSGIGGSFIGITYSTNNLFGYGQSVTVDIQAGNRQR
                     NVVLSYSEPYLFGKPIGLGVSFFSSRLNFVNGIASSGQLVNAGNNLFGLSGETLFTQS
                     TTGASLSLSAPLSVLTRRFSRFARFSRVGISYS"
     gene            complement(2239..>2466)
                     /locus_tag="PKZ53_13505"
     CDS             complement(2239..>2466)
                     /locus_tag="PKZ53_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41503.1"
                     /translation="WFLVLGSWFLVLGSWFFESIGDQVPSTKYQVPDVVKRKKEKEKT
                     GKNSTRQIGEPEEKCTIGNSQVQGDNIKQIF"
BASE COUNT          645 a          605 c          597 g          619 t
ORIGIN      
        1 acgagtagga aatcccgacg cgggagaagc gggcaaaccg ggaaaagcgg cgggtcaaaa
       61 ccgaaagcgg tgcactcaac gagagtgagg ccccggtggt tgactgggta aagagtgttt
      121 caccactcag tccaaacagg ttgtttccag cattgacgag ctgacctgac gatgcaatcc
      181 cgttcacaaa attcaaccga ctgctaaaga aggacacccc caacccaatt ggttttccaa
      241 agagataggg ttcgctatat gacagcacga cgttgcgttg ccggttcccg gcctggatat
      301 caactgtcac cgactggcca tacccaaaca ggttgttggt ggaataggtg atcccgataa
      361 acgaaccacc aatccctgag actccgccgg taaactggat ttgctgacga cctttttctt
      421 tgacgcggag gttgatgtct acttctttat tgcgttcgtc ggtttgaatc tgggcgtctt
      481 cttttttgat ctcttcaaag aatccaagct gattgagccg caaaatactg tattcccaca
      541 attcctggtt gtacggtgtt ccttcatcaa ccagcaattc gcggcgcagg acggtgtcac
      601 gggtaaccga gttccccaaa aattcaatgc gtcgaatcgt aaacaccgag ccttcagtga
      661 cgttgagggt aaaatccacc acaccttcct tggcatcagg tgcaactggt ttaaagtcct
      721 gattgagtga ggtttcagcc tggatgtatc cctggctgcc ataggctttt ttcaggcgtt
      781 catacacccc ttcgcggatc gttttgccgc tggcaatgtc accttttttc atgcctgaaa
      841 ccgccagtac ctgttccggg gtgaagatgg ttgagccttc gaccgacagg tccccaaacc
      901 gatacagaat gccttcatca atcggaatgg tgattttcaa ggcgcgactg ggtttgccaa
      961 agaaaatcca gcgactgcga accattccag cttcttcgac tttggcttct ccgatcactg
     1021 gtcgcaaata gccacgatcc agcaaaaact gacgcaccag gagcatatca ttctcaaatt
     1081 tgcctgggtc atagacgtct ttgctggtga aaatcgtgat caggctcgac tgcttggtca
     1141 gcttcatcgc gcctttcaac tggcggtctg aaaacacctt gttgccttca aaatcaattt
     1201 tggtcacccg gacccgggga ccttcgtcca cgttgaagac aatgaccacg gttgtggctg
     1261 aaatatcctc aatatccgct ttgacatcgg cttcaggtcg gcctttttcg ctcaacatgc
     1321 gttcgagttc atcttcagcg cgtcggacct tgaccgggtc aaactgggaa tctttggtca
     1381 aggagatgcc tttttcgcga tatcgggtga gcacatcgct caactgaacc gatttaagtc
     1441 cttcatactg aatatcgcga atgatagggc gctcgcgcag aacaaacaca atgatgacgc
     1501 cgccgcgaaa cccttcttcc acggtcacgc ggctctcaaa ttcatcgaaa aagccttcag
     1561 atacaatggc ttgatagtcg cgttcaacct gatcgcggtt ataaatatca ccaggtttgg
     1621 ttgagacccg caatcggatg gtatccgttg gaattcgacg gttcccacgg aattctacgt
     1681 cttcaacgat acgttgttga gccgattcat cttgtgggga acgaagggtg gaggaaaggt
     1741 gggaggctgc ggcttgaacc tggagtggag tgacgactcc cagcgaaaga agtgccaaga
     1801 aaaaccagcg tatgcgcata aaagtagagc tgtgtgccat ttgatcgtgt gctgccgttg
     1861 cagtgaacgt tcctgagctg attgccacca aaagtggtac gaccagcaaa aaaatgatca
     1921 ggatgaaagt gctgtttgat gatggtggcg ggtctttgga atcaagccca cctttgtgat
     1981 ttcaggttgt cgagcgaaca aagacgccag agtttaccca aatgccgctg acaatcaagg
     2041 gcaagatggt ttcaatcgaa ctccctgccc gaaaagaaaa aagaaaaagg aaaaaggaaa
     2101 aaggaaaaac atcgaaaaaa ccgtaatcgg gaattcggtg aggaggttca agtgactgaa
     2161 aatgaatcat tttcccaata ctgaagaccc ggtatcagcc ggagctgtgc cagtgtgatc
     2221 agaaaaaata tcgcattttc agaaaatttg cttgatgtta tccccttgca cttgagaatt
     2281 tccgattgtg cacttctctt caggttcacc tatctgacga gtgctgtttt ttcctgtttt
     2341 ttctttttcc tttttccttt tgactacatc tggtacttgg tacttggtac ttggtacttg
     2401 gtcaccaata ctttcgaaga accaagaacc aagaaccaag aaccaagaac caagaaccaa
     2461 gaacca
//
LOCUS       DAOQDO010000845         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37677_length_2466_cov_3.678557, whole genome shotgun sequence.
ACCESSION   DAOQDO010000845 DAOQDO010000000
VERSION     DAOQDO010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37677_length_2466_cov_3.678557"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKZ53_13510"
     CDS             <1..847
                     /locus_tag="PKZ53_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41504.1"
                     /translation="HAVFRRKLPHLETLSPAQFKLLEAVFQAMTEAIPENVQLNGEQI
                     TGSLATVYLTSPESGTESKEVVTVVNDKNNPMGVLKTETSDKAAPPGQNILEERDKNA
                     SIKPTVTAASPNAESKTGIIKTEGQTANQSSNVLPVNLILVNGQWKIGDAETQALVNS
                     FKDQYFFYELNGAFQQMQSNEGSIYNLLTTLVNVEMVYARSNNGFCASIPELVQEGIL
                     KDNVKATQMFGYRYEVVLSADRTSFVIYAEPMNYPLTGRFSYFADQTGVQMKEIGGAR
                     LQPGK"
     gene            1265..2029
                     /locus_tag="PKZ53_13515"
     CDS             1265..2029
                     /locus_tag="PKZ53_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ41505.1"
                     /translation="MKQALLNTIEYTVNPTAFSQMMIEPVEMNAEPESVELESEAEAI
                     ELETAPEGFDLVADLIEEPVVNQESLTGTLEEIYHKHYRRVYSLCLRMTSNAADAEDL
                     AHDVFIQLQRKLSSFRGESAFTTWLHRITVNQVLMHFRKKSYRSETVTEDGELPEVVQ
                     PSTKRPSMMPIIDRIALLRAIEQLPRGYRIVFIMHDMQGYEHEEIGQILGVSAGTSKS
                     QLHKARMRLRELLLKRVGVEAIEGLDDASLALLAQL"
     gene            complement(2193..>2466)
                     /locus_tag="PKZ53_13520"
     CDS             complement(2193..>2466)
                     /locus_tag="PKZ53_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ41506.1"
                     /translation="RDISAIVRRTLGLGTQTRGVLVTKVERGLPGDDAGIRREDIILA
                     IDSTPVVSSGQFNRLIARKNPGTKVNIRIWRNEQEYTVSAAVVEKK"
BASE COUNT          644 a          611 c          577 g          634 t
ORIGIN      
        1 gcacgcggta ttccgcagga agctgccaca ccttgaaact ctgtcaccag ctcaatttaa
       61 actgttggaa gccgttttcc aggccatgac cgaagccatt ccagaaaatg ttcaactcaa
      121 tggcgaacaa atcactggca gcctagccac cgtctatttg acctctcctg aatcgggaac
      181 cgaatcaaaa gaggtggtaa cagttgtaaa tgataaaaac aacccgatgg gcgtgctgaa
      241 aacagaaacc tccgacaagg cggctcctcc aggtcagaac attctggaag aacgcgacaa
      301 aaatgccagt atcaaaccaa cagtaaccgc tgcctcaccc aacgctgagt caaaaaccgg
      361 gattatcaaa actgagggtc aaacggcgaa ccaatcgtcc aatgtgcttc cagtcaatct
      421 gattctggtc aatggtcaat ggaaaattgg ggatgccgaa acccaggcac tcgtcaatag
      481 tttcaaagat cagtatttct tttatgaact gaacggggcg tttcagcaaa tgcagtccaa
      541 tgaaggcagt atttataatt tgctgacgac actggtcaat gttgaaatgg tctatgccag
      601 gtcaaacaac gggttttgtg cttcgattcc tgaactggtt caagaaggca tcctgaagga
      661 caatgtgaag gcaacccaaa tgtttggtta tcgctatgaa gtcgtgctgt cggcggaccg
      721 gacatccttt gttatttatg ccgagccgat gaactatccg ctgacaggtc gtttttccta
      781 ttttgccgat cagactggcg tccagatgaa agaaatcggt ggcgcccgat tgcagccagg
      841 caaataagtc ctctcatttc ccgcagtacc cgcatctctc ctcagctcct ctccaactcc
      901 aatctgtaca atttgagccg cacctgtctg cgtatgaatg cagacaggtg ctatcccata
      961 tcgtgtaatc ggacgaaagc cagtacacca aacacggtgt gcttttggcc tcactgaggc
     1021 aacctggtca gttcaggttg cttgtggtga cgggatgtaa gtatttacta actttttctt
     1081 ttttctgtaa tccactgagg aatcagccat ttagccagta tacgactcaa tccttacaaa
     1141 attttttcct gatttcatca gttttttgga gtttgtgcac caggtttgca ctaggtaaag
     1201 acactggctg atgaaaacac acacttattt tgatggcttt tatttcacat cggaggaaaa
     1261 caatatgaaa caggctctat tgaatacgat tgaatacact gttaatccaa ccgctttttc
     1321 acaaatgatg attgaaccag tcgaaatgaa cgctgagcct gaatcagttg aacttgaatc
     1381 cgaagcggaa gcaattgaac tggaaacggc gccggaaggg tttgatctgg tggcggatct
     1441 gatcgaagaa cccgtcgtga accaggaatc gctcaccggc acgctcgaag agatttatca
     1501 caaacactat cgccgggtgt acagcctctg tttgcggatg acttcaaatg cggctgatgc
     1561 tgaagatctg gcccacgacg tatttatcca gcttcagcgt aaattgagca gcttccgtgg
     1621 cgaatcagcc ttcaccacct ggttacaccg cattacggtc aatcaagtgt tgatgcactt
     1681 ccgcaagaaa tcctatcggt ctgaaaccgt gaccgaagat ggtgaactgc ccgaggtcgt
     1741 gcaaccgtca accaaacgac cgtcaatgat gccgattatt gatcgaattg cattgctcag
     1801 agcgattgag caattgcccc gtggttaccg aattgtgttc atcatgcacg atatgcaagg
     1861 gtatgaacac gaagaaatcg gacagatttt aggtgtgagc gctggcacct ccaagtctca
     1921 gttgcacaag gcccggatgc gcctgcgaga gttgctgctc aagcgggttg gtgtcgaagc
     1981 catcgagggc cttgatgatg ccagcctggc gctccttgcc cagttataat gacacacggc
     2041 acctgacggg ttctgcgatg agccattgat tgtatctggt ctgtgacttt ctccttgtaa
     2101 cgcgattcct ttcccttcct cttcatgtca aaagctgtgc ctcgactgta ggcacagctt
     2161 ttttttgttc agagttttct gacattgtct ggctatttct tttcaactac agccgccgaa
     2221 accgtgtatt cctgctcatt tcgccaaatt cgaatattga ctttggtacc ggggtttttg
     2281 cgggcaatca ggcggttgaa ttgtccactt gaaaccaccg gcgtgctatc aattgccaaa
     2341 ataatatctt cacgacgaat tccagcatca tcgccgggca gcccgcgctc gactttggtt
     2401 accaatacac cgcgagtctg ggttccaagc ccgagcgtgc ggcggacaat ggctgaaata
     2461 tcacgg
//
LOCUS       DAOQDO010000846         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37737_length_2464_cov_4.415940, whole genome shotgun sequence.
ACCESSION   DAOQDO010000846 DAOQDO010000000
VERSION     DAOQDO010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37737_length_2464_cov_4.415940"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKZ53_13525"
     CDS             complement(<1..286)
                     /locus_tag="PKZ53_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41507.1"
                     /translation="MTLENTNSEKTVLIRMYAQTDVGMVRQGNEDNFLVVDLSTAATW
                     TADHDAPPTELLSYTQGRYGSLFAVSDGMGGALAGEVASQLAVTAVRDWML"
     gene            complement(1137..2282)
                     /locus_tag="PKZ53_13530"
     CDS             complement(1137..2282)
                     /locus_tag="PKZ53_13530"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNJ41508.1"
                     /translation="MGIATDVIHAGQAPDPTTGSITVPIYQTSTYVQEGLGKHKGYEY
                     ARTHNATRAALEENIAILEKGVGACAFASGMAAIHAVMTTLLKAGDHVVVSDNTYGGT
                     YRLFDKVLTKFGLRFSYIDTTDPGVVADAIQPNTRMMFLETPTNPVMRLCDLRALSEI
                     ARPREIQVVVDNTFMSPYLQRPIECGADIVVHSTTKYLNGHSDSVGGVAVVTSKEVHE
                     KLAYIQNAAGAIISPFDAWLTLRGIKTLAVRMKQHDINGRAVAAYLSTHPKVTRLYYP
                     GLPTHPQYELACRQMDGFGAMLAFEASGGIDQARKVLEGLRVFALAESLGGVESLVCH
                     PASMTHASVPDEDRQRLGITDTLIRLSVGIEDGEDLIADLDQALALL"
BASE COUNT          629 a          627 c          591 g          617 t
ORIGIN      
        1 gaagcatcca atctcgaacc gcagttaccg ccaactggct ggcaacctca ccggctaatg
       61 ccccgcccat cccatcagac acggcaaaca gtgatccata tcggccttga gtataagaaa
      121 gcaactcggt gggcggtgca tcatggtctg cggtccaggt cgcagccgta ctcagatcaa
      181 ccaccagaaa attgtcttcg tttccctggc gtaccatgcc cacgtccgtt tgagcataca
      241 tcctgatcaa cacagttttt tcactatttg tattttcgag tgtcataggc aggcaagcct
      301 tacgtcaaat gattgcggtg tcagtgatcc ataaacagtg acgttattcc cacggtacat
      361 ggtgggaatt ccttagttga cctcatgccg atcaatctgg gagtcaggca aattttgacc
      421 tctgcctgca atttgacaaa aacaaccccg gcattgatgc gtcatcaagt cctgagtgaa
      481 ttaccctcag tgtgcagcca cggtgagggc aactctgatc ccttgaagtc agtgagttct
      541 ttttacctat ggttgtctga ttaggcaacc ctaaaaagag gagacagtct tcatttcagc
      601 ctgaaggtgc agaaacttca aacctgtctt tctttcaagc aaaacccgtg gatagtgcaa
      661 ctggcagatg cgagtgatct aaaacttccc tggtgatgaa gagtgcccag gtacattctg
      721 cttttcctga aaaaaccaga gccgatttga cctgtttttt tctatcattt gatctcaaac
      781 aaggttggat catcgagatt tggaaaaaaa gaacctgctg ggacggaaac gtgatgttgg
      841 aaatgatctc aaaatgaatg tagagctggc ttcaggttga gcagtgtgaa gagtatgtca
      901 ccctattcgc cgccgattat agccaagctt tttctgggag cctagcgtga aatcagttca
      961 cattgaaaaa cagttgcctc tggcggttct aaagagctga ctggctggct gatcagatga
     1021 aactcaccct gagttgtggc caaattcgtg gtgagttgag gttatatcag acggctggaa
     1081 ctcgatgaac tgaacgcatt ttctcgaaac aaccgactgg cgaacaagag cagattctaa
     1141 agcaatgcca gcgcttgatc cagatctgca atcaagtctt ccccatcttc aatcccaacc
     1201 gataagcgaa ttaaggtatc agtgatgcca agccgttggc gatcttcatc aggcacactg
     1261 gcatgggtca ttgacgccgg atggcaaacc aggctttcaa cccctcccag agattctgcc
     1321 agtgcaaaaa ccctcaaccc ttcaagcact ttccgggctt gatcaatacc gcctgacgcc
     1381 tcaaaagcca gcatggcccc aaacccgtcc atttgccgac aggccagttc atactgtgga
     1441 tgggttggaa gtcccggata atacaggcgg gttacttttg gatgggtgct cagataggcg
     1501 gcaacggccc gtccattgat atcgtgttgc ttcatgcgaa ccgccagggt tttgatcccc
     1561 cgcaaggtca accaggcatc aaatggcgaa ataatggccc cagccgcgtt ctgaatatag
     1621 gccagttttt cgtgaacttc tttggaagtc accaccgcca cgcctcccac actatcgctg
     1681 tggccattga gatatttggt cgttgaatga accacaatat cggcaccaca ctcaattggt
     1741 cgctggaggt acggtgacat aaaggtgttg tcaaccacca cctgaatttc ccgtggtcga
     1801 gcaatttctg aaagcgcccg caagtcacac aaccgcatca ccgggttggt tggtgtttcc
     1861 aaaaacatca tgcgggtgtt tggctggatg gcatcagcca caaccccagg atcagtggta
     1921 tcaatataac tgaaccgcaa tccaaatttg gtcaatacct tgtcaaacaa gcgataggtg
     1981 ccgccatagg tattgtctga aaccaccacg tgatcgcccg ctttgagcaa tgtcgtcatg
     2041 acggcgtgga tggcagccat ccctgaagca aacgcgcagg ccccaactcc tttttccaga
     2101 atggctatat tctcttcgag cgctgcccgc gtggcattgt gtgtccgggc atactcatag
     2161 cctttgtgtt tgcccaggcc ctcctggaca taggttgagg tttgataaat tggaacagtg
     2221 atcgaaccag tggttgggtc tggggcttga cccgcgtgaa taacatctgt tgcgattccc
     2281 ataattcatt ctttcgccag cgactgaagg tcaacctgga tcaacgccaa ttgttgagag
     2341 agaacacagg ttacttctgc tggctggtgg aggggggtct cattcatcaa aaagataatt
     2401 cgagatgagg tggttttgac accaggttat tcgtcaaaaa tacccttgct catataccgt
     2461 tcag
//
LOCUS       DAOQDO010000847         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37781_length_2463_cov_3.279070, whole genome shotgun sequence.
ACCESSION   DAOQDO010000847 DAOQDO010000000
VERSION     DAOQDO010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37781_length_2463_cov_3.279070"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..2248
                     /locus_tag="PKZ53_13535"
     CDS             128..2248
                     /locus_tag="PKZ53_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNJ41509.1"
                     /translation="MAIHFEKDAQNIVTLTMDMPNRSANVINQEFGEALATALQKLKA
                     EDALAGVIVTSAKKTFLAGGDLEMLMAATDPGEAFTAAEALKACFRQLETLGVPVVAA
                     MNGAALGGGCELALACHYRIALTTPDVQIGMPEVTLGLLPGAGGVTRLTRLLGLQEAF
                     PYLMEGKKVSAKSALKAGIIHELASDRDDMVTKARAWIAANPNAAQPWDKSGYKMPGG
                     DPRNPKVAQMISVAPAMLLQKTHGNYPAPLAIMSAAVEGALVDFDTASRIESRYLAQI
                     ATGKVAKNMITAFWTQLNQINAGGSRPAGFAPSKVKKLGVLGAGMMGSGIAYAAAICG
                     IPVVLKDVSLETAEKGKGYSAKLLEKKVSQGRMKPEERDGILALIHPTADLEDVRGCD
                     LIIEAVFEDRAIKAKVTEEAESRIEETAIYASNTSTLPITGLAQASSRPANFVGLHFF
                     SPVDKMQLVEIIRGKETSPETLARSFDFVRQIKKIPIVVNDSRGFYTSRVFGTYVNEG
                     LALLAEGNHPQAIEAAGIQAGMPVGPLAVSDEVSMSLMLHIRTQTEKDFAAEGKTAPS
                     HPGHRVVDRMVKEHGRAGKAAGKGFYEYPKEGKKYLWPELKTLFPATGEPLSQAEMID
                     RIRFAQVVETVRCLDENVVTTVADANIGSIFGWGFAPFKGGTLQFINDYGVSAFVARA
                     TELAARYGDRFAPPTEARLRELLG"
BASE COUNT          590 a          653 c          695 g          525 t
ORIGIN      
        1 cgatttggtc tctattcccc ggttgaatct gttttgtcca caaggcattg catgatgtcc
       61 ttgcaggact caatccttga tctggcacat atttgctgaa ccctgaaccc tgaaccctac
      121 tttccttatg gccattcatt ttgaaaaaga cgctcaaaac attgttacct tgactatgga
      181 catgccgaac cgctcggcca atgtcatcaa ccaggaattt ggcgaagccc tggctacggc
      241 cctccaaaag ctcaaagcgg aagacgcgct ggccggggtg attgtcactt cggccaagaa
      301 aacgtttctg gctggcggcg atttggaaat gttgatggcg gcaacggatc caggcgaagc
      361 gtttactgcc gccgaagcct taaaagcctg ttttcgtcaa ctcgaaacgc tcggggttcc
      421 ggttgtggcg gccatgaacg gcgcggcgct tggcggaggc tgcgaactgg cactggcctg
      481 tcattaccgg attgctctga ccacgccgga tgtccagatt ggaatgccgg aagttacgct
      541 gggtctcctg cctggtgccg gtggcgtcac ccgattgacc cggttgctcg ggttacagga
      601 agctttcccg tatttgatgg aaggcaagaa ggtttcagcc aaatcagccc tgaaagccgg
      661 aatcatccac gaactggcat cagatcgcga cgacatggtg accaaagccc gagcctggat
      721 tgcggccaat ccaaacgcgg ctcaaccgtg ggataaatcg ggctacaaaa tgccgggcgg
      781 tgacccgcgc aatcccaaag tcgcccagat gataagcgtc gcaccggcga tgttgcttca
      841 aaaaacgcac ggtaactatc cggcgccgct ggcgattatg agtgcggcag ttgaaggggc
      901 gctggtggat tttgatacgg cttcgcggat tgagtcacgc tacctggccc agattgcgac
      961 cggcaaagtc gccaaaaaca tgatcaccgc gttttggacg caactcaacc agatcaacgc
     1021 tggtggcagt cgtcccgccg ggtttgcccc atcgaaagtc aaaaaactgg gggtgctggg
     1081 tgctggaatg atggggtctg gcattgccta cgctgcggct atttgtggca ttccagtggt
     1141 gctcaaagac gtctcactcg aaacggctga aaagggcaaa ggctatagtg ccaaattgct
     1201 tgaaaagaaa gtcagccagg gtcggatgaa gcccgaagaa cgcgatggaa ttctggcatt
     1261 gatccatccg acggcggatc tagaagatgt gcgcggctgt gatttgatca ttgaagccgt
     1321 gtttgaagat cgtgcgatta aggccaaagt caccgaagaa gccgaaagtc gaatcgaaga
     1381 aaccgccatt tatgcttcaa acacttcgac gctgccgatt acggggctgg cccaggcgtc
     1441 ttcccggcca gccaacttcg tcgggctgca cttcttttcg cccgtggaca agatgcaact
     1501 ggtggaaatc attcgcggca aagaaacttc acctgaaacg ctggcccggt cctttgattt
     1561 tgtccgtcaa atcaagaaaa tcccgattgt ggtcaatgac agccgtgggt tctataccag
     1621 ccgcgtgttt gggacctatg tcaacgaagg gctcgccctc ctggccgaag gaaaccaccc
     1681 tcaggccatc gaagccgccg ggattcaggc cggaatgccg gttggcccgc tcgcggtctc
     1741 tgatgaagtc agcatgtcgc tgatgctgca cattcgaact caaaccgaaa aggattttgc
     1801 ggcagaaggg aaaacggctc cctcacatcc tggccatcgg gtggttgata gaatggtcaa
     1861 agagcacgga cgcgctggaa aagccgccgg aaaaggattt tacgagtatc caaaggaagg
     1921 caaaaaatat ctctggccag agttgaaaac actctttcct gccactgggg aaccactttc
     1981 acaagctgaa atgattgatc ggattcggtt tgcacaggtg gttgaaaccg tgcggtgcct
     2041 ggatgaaaac gtcgtgacca cagtggcgga cgccaatatc ggcagcattt tcggctgggg
     2101 gtttgctccg tttaaaggcg gcacgttgca attcatcaac gattatggcg tttcggcttt
     2161 tgtcgcacgg gccactgaac tggcagccag atacggagac cggtttgcgc ctccgaccga
     2221 agcccgcctg cgtgaattat tgggctgaga aaaccagggc tgagaaaacc agggctgaaa
     2281 aaaccagggc tgaaaaaacc agggctgaag actcgcaagc tcggggctga agacatcggg
     2341 ctcggggctg aaaaaaacgg gttcaatccc ctgagcccca agcccgacgt cttcagccca
     2401 atgtcttcag cccgatgttt tcagcccgac gtcttcagcc caatgtcttc agcccgacgt
     2461 ctt
//
LOCUS       DAOQDO010000848         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37808_length_2462_cov_3.691732, whole genome shotgun sequence.
ACCESSION   DAOQDO010000848 DAOQDO010000000
VERSION     DAOQDO010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37808_length_2462_cov_3.691732"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKZ53_13540"
     CDS             <1..591
                     /locus_tag="PKZ53_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ41510.1"
                     /translation="RMVDRLMASFNDILEMLRINEGRIALRPASFDLSNLVKKVLEEL
                     EAFIEQRRLQVTVEVPPELYVTADPEKIELVLINIIQNAIKFTFDDGDIVIRATHNGH
                     NEAHILIRDSGIGIDQVEIKRIFETFYTSSDPSTHTSGKYEFSARGTGLGLSIARSYI
                     EAHGGRVWAESEGKGHGSTFHIILPVEVTTKPKNEE"
     gene            786..2177
                     /locus_tag="PKZ53_13545"
     CDS             786..2177
                     /locus_tag="PKZ53_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41511.1"
                     /translation="MSSSLDQTPSTGGSYPQCTTCGRKLREWSRFCTFCGARMEQIPT
                     GDLRLPQISQKSGDLPSTEVFASTNARVPSEPLPHTSEVFIVPGYQTNGLAQTEPMGQ
                     PVAPSRERTTAPIPQLIIADRPGQLRQMVAIVCVCLAVGLTLAGVFFSRKGPHSNSPS
                     VQPSIVNSEFEHVPSPEELAAAHQKGAGVPAKSGTSANPTGTQSDSGTSQPGGGTDSE
                     ETMGADPTSPGTGGGSSSGPSQQSPAGSTSGTSTSSQPSSSTGQSSIPDGNNEPPSKP
                     SASQKSTSGGTIIPAGSNSRSNPTATPANDSSSPSRRSKRSESDANSVIVATNRRQDE
                     ETEVPSPMKRRRSQDAESSDPDDEYSSDAHSRRGRDWPRERRRDEDSRQDVMSYPRNG
                     IAVWTGAAGRSQRVTVYGFPGIPISVNRPAVRGGHARMITPPSPSNGWKYAVVEFERG
                     EGTCEVAIPWQKL"
     gene            complement(2245..>2462)
                     /locus_tag="PKZ53_13550"
     CDS             complement(2245..>2462)
                     /locus_tag="PKZ53_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41512.1"
                     /translation="EISAFEVKRLAKIGYPYNGQERPIIRLACKAICSGSVTIVIPPW
                     NGQIAPLYRNNQPEPPVAEPATAKMTK"
BASE COUNT          605 a          683 c          631 g          543 t
ORIGIN      
        1 cgcatggtgg accgcctgat ggccagtttt aacgatattc tggaaatgct gcgcatcaat
       61 gaaggccgga ttgcgctccg cccagcttcg tttgatctga gcaacctggt gaagaaggtg
      121 ttggaagaac tcgaagcctt tattgagcaa cgtcggttac aggtcaccgt ggaagtaccg
      181 cctgagttat atgtcaccgc tgaccccgaa aaaattgaac tggtcttgat caacattatt
      241 caaaatgcca tcaaattcac ttttgatgac ggagatattg ttattcgagc cactcacaat
      301 ggccacaacg aagctcatat cctgattcga gactctggga ttgggattga ccaggttgaa
      361 atcaaacgca tttttgagac gttctacacc agttctgacc catcaaccca cacttcagga
      421 aaatatgaat tctcagcccg tggtacagga ttaggactct caattgccag aagttacatc
      481 gaagcccacg gaggccgcgt ctgggctgaa tctgaaggca aaggccacgg cagtaccttc
      541 cacattattc tgccggttga ggtcaccact aaaccgaaga atgaagaatg aagaacgctt
      601 cccgtttccc gctctgggca acctttcaca tcacaccagg cttctcacgc ccagtcattc
      661 acgcctcctc atttttctca catacatctt tatagtcccg gcaacactgc gtgggcacct
      721 ggtcacggtg gcaactcggt gcggcaggca agcccggagt caaaaacaca aggtcagggt
      781 gttctatgtc aagctcattg gatcaaacac catcaacagg cggatcttat ccacaatgta
      841 ccacgtgcgg tcgcaagctc cgtgaatggt cacggttttg cacattctgt ggtgcccgga
      901 tggaacaaat tccgaccggt gatcttcggc ttccccaaat ctctcaaaaa tctggggact
      961 tgccatccac tgaggttttc gcttcaacca acgccagggt tccaagcgag ccactccctc
     1021 acacctctga ggtgtttatt gtaccggggt atcaaaccaa cggtctggct caaacggaac
     1081 cgatgggcca gccggtggcg ccctcgcgcg aacgaacgac ggcgccgatt ccgcaactga
     1141 tcattgctga tagaccaggt cagttgcggc agatggtggc gattgtatgt gtgtgtctgg
     1201 cggttggact gacgctggca ggtgttttct tctcgcgaaa aggcccacat tcaaattccc
     1261 cgtcggttca gccatcaatt gtgaacagcg agtttgagca cgtcccatca cctgaagaac
     1321 tggccgccgc ccatcaaaaa ggggctgggg taccagccaa atccggtact tcggcaaacc
     1381 caaccggaac tcaatccgat tcaggaacgt cacaacctgg aggcggaacc gattccgaag
     1441 aaacgatggg ggctgaccca acctcccccg gcacgggtgg cggctcatcg tctgggccat
     1501 ctcagcaaag cccagccgga tcaacttccg gcacatcaac gtccagccaa ccgtcatcct
     1561 ccacagggca gtcatcaatt cctgacggaa acaacgagcc gccatccaaa ccttctgcct
     1621 ctcaaaagtc aacctctggc ggtacgatca tcccggcggg aagcaactcc agatccaatc
     1681 caaccgcaac cccagccaat gattcatcat cgccctccag gcgctctaaa cgctctgaat
     1741 ctgatgccaa ctcggtcatt gttgcaacca atcggcggca agacgaggag actgaagttc
     1801 catcaccgat gaaacggcga cggtcacagg atgcagagtc atctgatccg gatgatgaat
     1861 attcctccga cgctcattcg cgccgtggtc gagactggcc acgtgaacgc agacgggatg
     1921 aagactcgcg tcaagacgtg atgagttatc cgcgaaacgg aatcgcagtg tggaccgggg
     1981 cggctggacg cagtcaacgg gtcacggtct atggatttcc aggaattcca atttcagtca
     2041 accgtccggc tgttcgtggc ggccatgccc gaatgattac cccgccatca ccaagcaatg
     2101 gctggaaata tgcagttgtg gagtttgaac gcggtgaggg aacctgtgag gtggcaattc
     2161 cgtggcagaa gctgtgatga tcacaactga taaactaacc gggtgaccaa ctgacacatg
     2221 gtcacgtggt cacccggtca gttgttattt tgtcatctta gcggttgcgg gttcggcaac
     2281 aggtggttct ggctggttgt tgcggtacag gggtgcaatc tggccattcc agggcggaat
     2341 gacaattgtg accgagccac tgcaaatcgc tttacaggcc agtcgaatga ttggacgttc
     2401 ctgaccgttg tatggatacc caatcttggc cagtcgtttg acttcaaagg ctgagatttc
     2461 at
//
LOCUS       DAOQDO010000849         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37838_length_2461_cov_2.988778, whole genome shotgun sequence.
ACCESSION   DAOQDO010000849 DAOQDO010000000
VERSION     DAOQDO010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37838_length_2461_cov_2.988778"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1293)
                     /locus_tag="PKZ53_13555"
     CDS             complement(238..1293)
                     /locus_tag="PKZ53_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNJ41513.1"
                     /translation="MRRLWKPTWRPVRNLSIALMLGLTLAALTACGGSGKTTLLVYTP
                     HGPELLKEFEKRFETANPTIDVQFQDLASQDILNRLTLERANPQADVWWGASSLTFDL
                     GVQEGVLETYKPTWGGLVKPTMHDAQDRWYATYLTPEVIVYNKELVPEAEVPKDWDDL
                     LSERWQGKVLIRQPLQSDTMRTIFGAMILRFWATTNGPQGGYDWLKKLDANTKSYPTD
                     GTQLMEKIGRGEGLVTLWNLPDVVLHQQKKLPLAFRYPESGTPIVTDGIGIVKGTRNL
                     EAAKRFYEFVTTPESLIFAANQFARVPVRRSDLDLNQLPESMRTEIKELPLDYSLLQK
                     EVKNWMQHWENNIRKRS"
     gene            2068..>2461
                     /locus_tag="PKZ53_13560"
     CDS             2068..>2461
                     /locus_tag="PKZ53_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41514.1"
                     /translation="MFHKSLVLLSLLLLLVSVSFGQSGRGRPQPPQPKPPVSTKPTPT
                     PPTGTTPSADGKIAPGGQLSDISQVGLTNRYVFRNGLTVILSEDHSTPIVSVLAFVKA
                     GTTDEPETARGVARVTGEVLLRGTTAHPG"
BASE COUNT          582 a          594 c          590 g          695 t
ORIGIN      
        1 atgaagaatg aagaatgaag aatgaagaat gaagaatgaa gaatgaagaa actgttggtg
       61 tttggtattg agcgcctggt acttagtgct tagaaatcaa ggttttaaaa actaataacc
      121 agttgccagt caccacgtat attcttcgtt ctcaattctt cattttcaat tcttcattct
      181 gcattctcaa ttctgcattc tcaattcttc attctgcatt ctcaattctt cattcaatca
      241 gcttcgcttt cggatgttgt tttcccagtg ttgcatccag tttttcactt ctttttgaag
      301 caggctgtaa tccagtggca gttccttgat ttcggtgcgc attgattctg gcaactggtt
      361 gagatccaga tcgcttcggc ggacaggaac gcgggcaaac tggttggcag cgaaaatgag
      421 actttccggc gtggtgacaa attcatagaa gcgtttggcg gcttccagat tgcgggttcc
      481 tttcacaatt ccaatgccat cagtaacaat cggtgtgccg ctttccgggt aacgaaaggc
      541 cagcggcaat tttttctgct ggtgtaaaac cacatccggc agattccaga gggtcaccag
      601 cccttcgcct cgcccgattt tctccatcag ttgagttcca tctgttgggt agcttttggt
      661 attggcatcg agttttttga gccagtcata accgccctgc gggccgttgg tcgtagccca
      721 aaaccgcaaa atcatagcgc cgaaaattgt ccgcatggta tcggattgga gtggctgccg
      781 gatcaagacc ttgccttgcc agcgttcgct caataaatca tcccaatctt tggggacttc
      841 ggcctcaggc acaagttctt tgttatatac aatgacttct ggtgtcaaat aggtcgcata
      901 ccagcgatcc tgcgcatcgt gcatggtcgg tttgaccagt cctccccagg ttggtttata
      961 agtttcaagg acaccttcct gaacacccag gtcaaaggtc agggacgatg ctccccacca
     1021 gacatcggcc tgcgggttgg ctcgttcaag cgtgagccga ttgagaatat cctgagaggc
     1081 cagatcctga aactgaacat caatggttgg gttggcggtt tcaaaccgtt tttcaaactc
     1141 tttcaggagt tcagggccgt gtggggtata aaccagcaag gttgttttcc cacttccacc
     1201 gcaggcagtc agtgcggcaa gggtgagtcc cagcatcaag gcgatgctga gatttctgac
     1261 tgggcgccag gtgggtttcc aaagtcgtct cattttttta tgtctcctct ttcgtgttgg
     1321 ggctgagaaa accagggctg ggggctgacg actcgcaagc tcagtactga agaaaatggg
     1381 ttcaataccc tgagcccgac atcttcagcc cgatgttttc cgcccgaggt tttcagttcc
     1441 aagtaaatgt caattacgaa ccgcttgtcg aatcatagtg ccggatgtca ccaaagactt
     1501 ttcaacttgc accggctggc ggcattatcg ccacgcattg tgaacagtga tttcctctga
     1561 attgattgtt tcaggtgtaa tgcttgtgag ttagtgtaag aaagtttttg gtgtaacagc
     1621 gagttctcct ctcggttcta cactgtcaat tttaagagac ctgccgcctc caaacaggta
     1681 atggaaggct ggcaggtctc attttttttt gataagtcaa ttgtcagtcg tcagtcgtca
     1741 gtcgtcagtc gtcgagtgtt gggattttga agtgtctgtt aagtctattt agttctattt
     1801 atgcaattta tctatgcttc ttcccaggaa atcatcaaac tgaagtacct gggtttggtc
     1861 tactgactta tcaacaactg acgactgact tatcaacgac tgactggtat aagtactgac
     1921 cactaacgaa aaactgttat aattcacaac ccacaatcta tcattgaaat caaagtttgt
     1981 gagttgagtc tccaaccctg aacccaaacc cccaaatcct aacctccaaa ttcgtgagcc
     2041 ctgaatccca ttccgaggtc aacgcccatg tttcacaaat cgcttgtttt actgtcattg
     2101 ttgttgttgc tggtatccgt ttcatttggt cagagcgggc gtggccgacc tcaaccacca
     2161 caaccaaagc caccagtttc gacaaagcca acacccacgc ctcccaccgg gaccacacct
     2221 tccgctgatg ggaaaattgc accaggtggg cagctttccg acatttccca ggttgggttg
     2281 accaaccggt atgtgtttcg caatggattg acggtgattt taagcgaaga ccacagcacg
     2341 ccgattgtca gtgtgctggc ttttgtgaag gctggaacga ccgatgaacc ggaaacagct
     2401 cgtggcgtgg cacgggtgac gggtgaggtg ctgttgcgcg gtacaacggc acatcctggt
     2461 g
//
LOCUS       DAOQDO010000850         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37840_length_2461_cov_2.803408, whole genome shotgun sequence.
ACCESSION   DAOQDO010000850 DAOQDO010000000
VERSION     DAOQDO010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37840_length_2461_cov_2.803408"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKZ53_13565"
     CDS             complement(<1..244)
                     /locus_tag="PKZ53_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HNJ41515.1"
                     /translation="MNMNLDPEICYTALKSRDARFDGKFFTAVKTTGIYCRPICPAIT
                     PQKQNCTFYTSAAAAQEAGFRPCLRCRPEVAPGMALQ"
     gene            549..1388
                     /locus_tag="PKZ53_13570"
     CDS             549..1388
                     /locus_tag="PKZ53_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ41516.1"
                     /translation="MENILGTHQFLEHKGCRLAFRVSGAGIPVLLIQGVAVHGDGWIP
                     QIKGLADRFQCLSFDNRGMGKSQPIAVSLTVEQMAEDAQVLVDAQGWKDVHVVGHSLG
                     GPIALQFALTNPKRVRSLSLLCTFARGKDAAPPTPWMMWVGMRTRVGTRRQRQRAFLQ
                     LVMPPEALADANHDALAEELEPLFGHDIADQPPATMKQLAAMRAYDATSRLGELSGIP
                     TLVVNARHDPIAPPKLGRRLAEGIPDATFVELADASHGVTIHQATRINGMLREHFLKA
                     DSK"
     gene            1437..1955
                     /locus_tag="PKZ53_13575"
     CDS             1437..1955
                     /locus_tag="PKZ53_13575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNJ41517.1"
                     /translation="MTQALLVIDLQNDYFPDGKFPLWNTEAVLNNIEQAISKASARQI
                     PVIIIQHIASSERGIAPFFNQGTTGAEVHPRILAAAPDAPIVVKEFADSFVNTTLEET
                     LSRLGVTELLVCGMMTQNCVTHTAISKSAEKYQVTILPDCCTTVNEMLHQIALHAVST
                     RVPLVPASEVFA"
     gene            2089..>2461
                     /locus_tag="PKZ53_13580"
     CDS             2089..>2461
                     /locus_tag="PKZ53_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41518.1"
                     /translation="MKSLEQISDFITDRIAAIYERPLPFGGTPDSVELVLHCYHELWA
                     EIHDTKEEYNSVRRKIYAEQDCGSMSFAFRHKRLNQGATTDEIAAYTVGQWQRISQEL
                     GIPISRQPASGGDVADAAQDIG"
BASE COUNT          565 a          624 c          682 g          590 t
ORIGIN      
        1 gctgaagggc cattccagga gcgacttctg ggcggcagcg cagacaaggc cggaatccgg
       61 cttcctgggc cgccgccgcg cttgtgtaaa aagtgcagtt ttgtttctga ggcgttatcg
      121 ccggacagat cgggcgacaa taaattccgg tggttttgac tgccgtgaaa aactttccat
      181 caaaccgcgc atcgcgcgac ttcaaggcgg tgtagcaaat ttctggatca aggttcatat
      241 tcatcttgtg tgtagccaat cgtgaggacc aggtagtaca gtggcaacct tctgattgcc
      301 ggctcttgtt tcgatgtcag agttatactc gatttcttct caggaaactg gccattttcg
      361 gacctgtaac ctgtggagtg cggcggcttg acgccgcttt ctgtgtggag tgcggcggct
      421 tgacgccgct ttctgtgtgg agtgcggcgg cttgacgccg ctttctgtgt ggagtgcggt
      481 ggcttgacac cgctttctgt gtggagtgcg gtggcttgac accgctttgg tataaggaga
      541 attgaacggt ggaaaatatt ctgggaaccc accagtttct tgaacacaaa ggctgccggc
      601 tcgcctttcg cgtttctgga gccggaatac cagtgttgct gatccagggc gtagcggttc
      661 acggggacgg ctggatcccg caaatcaaag ggcttgctga tcggtttcag tgcctttcgt
      721 ttgataatcg cggaatggga aagagtcagc cgattgccgt ttccttgacc gttgagcaga
      781 tggcggaaga tgcccaggtc ctggtagacg cccagggatg gaaagatgtg cacgtagttg
      841 ggcattcgct gggtggcccg attgcactcc aatttgccct caccaatccg aagcgggtgc
      901 ggagtctttc attgctctgc acgtttgcac ggggaaaaga tgcggcccct ccgacgccgt
      961 ggatgatgtg ggtgggcatg cgaactcggg tggggacgcg gcgtcagcgg cagcgggcat
     1021 ttttgcaact ggtgatgcct cccgaggctc tggccgatgc caatcacgat gcgctcgccg
     1081 aagaacttga accactcttt ggacatgaca ttgctgacca gcctccggcg accatgaaac
     1141 aactggcggc catgcgagct tatgacgcca cctccagatt gggagagctt tccggaatcc
     1201 ctacgctggt ggtcaatgcc cgacacgacc cgattgcacc gcccaaactg ggccgacgcc
     1261 tggccgaagg aatcccagac gccacatttg ttgaactggc tgatgcctcg cacggcgtga
     1321 ccattcacca ggcaactcga atcaatggta tgttgcgcga acattttctc aaagctgact
     1381 caaagtgagt ttgagaatta aatgaaaaaa cataaatcca actaggaatt aattttatga
     1441 cccaagcact tctcgtaatt gaccttcaaa acgactactt tccagatgga aaattccctc
     1501 tgtggaacac cgaagccgtc ctcaacaaca ttgagcaggc gatttccaaa gccagtgccc
     1561 ggcagattcc ggtgatcatc attcaacata ttgccagcag tgagcgggga attgctcctt
     1621 tcttcaacca gggaacaacc ggcgccgagg ttcaccctcg gattctggct gccgcgcctg
     1681 atgccccgat tgtggtcaag gaatttgcag atagctttgt caacacaacc cttgaagaaa
     1741 cgctttcacg ccttggcgtg actgaactcc tggtgtgcgg gatgatgacc caaaattgtg
     1801 tgacccacac tgccatttca aaatcggctg aaaaatatca ggttacgatt ttgcctgact
     1861 gctgcacgac ggtgaatgag atgcttcacc agattgcgtt gcatgctgtt tcgactcggg
     1921 tgccgctggt tcccgcgagc gaagtgttcg cctaaagtca aaaaatttgg agtgcgatga
     1981 cctgtcatcg cttttatatg aggcgacttg tcgctggaaa ctaaagtgct gacaagtcag
     2041 cgtactccaa aagatgcctg cctgaaatat ggaaactgac tgacgagtat gaaaagctta
     2101 gagcaaattt cagattttat aactgaccga attgctgcta tttacgagcg acctttgccc
     2161 tttggcggta caccggatag tgttgaactg gtccttcatt gttatcacga attgtgggca
     2221 gaaattcatg atacaaaaga ggagtacaac tcagtcaggc gaaagattta tgctgagcaa
     2281 gactgtggct ccatgagctt tgctttccga cataaacggc tcaaccaggg ggcaacaacc
     2341 gacgagattg ccgcttatac cgttggccaa tggcagcgta tttcccagga gcttggtatc
     2401 cccatttcac gtcagccagc cagcggaggt gacgtcgccg acgccgcgca ggacataggc
     2461 g
//
LOCUS       DAOQDO010000851         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37842_length_2461_cov_2.640898, whole genome shotgun sequence.
ACCESSION   DAOQDO010000851 DAOQDO010000000
VERSION     DAOQDO010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37842_length_2461_cov_2.640898"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2461)
                     /locus_tag="PKZ53_13585"
     CDS             complement(<1..>2461)
                     /locus_tag="PKZ53_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41519.1"
                     /translation="SHYYDVTIWNLAESGRIGTYNYSSGANTSYARGTFKLVPPVWWH
                     FFKVRDEAGSLALRRVSDETAHQLFDLGKENVSNPEADLQQRLDSLVEKLPQYLPELT
                     HSNLKRGVAGIILEAATLNTKLHKLVSPPNPAPVAETENLDLIPFPLDAKVNLALKPF
                     GIDSTPNQGKVIGKHIGEISDWFRAAYLGNQDELEKLETRSLTWSTFHFIRLMGKIGA
                     PAFVAAVNYQSLLDGDQVHCGEILDFLEGWATTIFADDSLQFRFFKGTCTGSGEEDAK
                     WQKPGSFWTFQQHGSIYFTRVAREWYANEYKMTFSALELTTTGEFNPLLHTITHESVP
                     ITGWSGTERIRTLVELVRQRGRIPWDEAIAQTLSQKTGLSRTAAILLWFGCLHFDVSW
                     YESNFLPQAFLTQFGLKKGAASAAREEELRAACSLEHSIALYTGIMPEAVETLWHPLG
                     NGPDDETSLVSRLACAWNDRFGKRIPISEALRTLIQKKLRGVPFKNSLMLSLISTPEA
                     SGYLRTDLIWEIQSNWSYYSNVCKATRPLLPDEHLLNPDWRTRHFSNEVATAVAHWLP
                     FLFENLPVGDPLRTNCAQVFNLTLERLKNPEFLVDLGFRQISSQNEGNIALRAFGDTP
                     YAGFENQPVAGLTGRDSGAMVAYVKMQPTGDSGYFGVCFRPAMVTDFEALTRLSTSTD
                     FIARPHHIGILKSAGMAAMAERIGSTPVPAGQWEPNPFYSAPELVTDVKTTLAVSEDA
                     ATLYLQVLALAEPTTTNLKKWNGWEAKRIKKAVAELVAKSLLIEARRPRAGRTFFLPG
                     GWTVLTGANLPLETWKLPLYAI"
BASE COUNT          566 a          626 c          663 g          606 t
ORIGIN      
        1 gattgcatag agcgggagtt tccaggtttc aagcgggaga ttggcgcccg tcaagacggt
       61 ccagccacca ggaaggaaga aggtgcgtcc ggctcgcggg cgtctcgctt caatcagaag
      121 cgactttgcg accagttcgg ctaccgcttt tttgatgcgt ttggcttccc agccattcca
      181 ttttttgagg ttggtggtgg ttggctcagc cagcgccaga acctgcaggt acaacgtcgc
      241 ggcatcttcg ctgactgcaa gtgtggtttt gacatcagtc accagttccg gagccgaata
      301 gaacggattg ggttcccatt gtcccgccgg caccggcgtt gacccgatgc gctcagccat
      361 cgcggccatg ccggctgatt tgagaatacc aatgtgatgt ggtcgggcaa taaagtcggt
      421 gctcgttgaa agccgggtca gggcttcaaa atcggtcacc atggctggtc ggaaacaaac
      481 cccaaaatac ccactgtccc cggttggctg catcttgaca taggcaacca tcgcccctga
      541 atcacggcca gtgagaccgg caactggctg attttcaaat ccagcgtagg gtgtatcacc
      601 aaaggcccga agcgcaatat ttccttcatt ttgtgagctg atctggcgga atcccaggtc
      661 tacgagaaat tcagggtttt tgagtcgttc cagcgtcagg ttgaaaacct gagcacagtt
      721 tgtccgcagc ggatcgccaa ccggcaggtt ttcaaacaaa aacggcaacc agtgggccac
      781 ggcagttgcc acttcattgc taaaatgtct ggttcgccag tcgggattca acaggtgttc
      841 gtcaggcaac agcggtctgg ttgctttaca cacattggaa taataggacc agttgctttg
      901 aatctcccag atgaggtctg ttcgaaggta cccggaagcc tcaggcgttg aaatcagact
      961 cagcatcagg ctatttttga acggtactcc cctcagtttt ttctgaatca gtgttcgcaa
     1021 tgcctcagaa atgggaatgc gttttccaaa ccggtcgttc caggcacaag ccagacgcga
     1081 caccaggctg gtttcatcat ctggtccgtt accgagtgga tgccacaatg tctcaaccgc
     1141 ttccggcatg atcccggtat agagggcgat actgtgttca agggaacagg cagcacgtaa
     1201 ttcttcttca cgggcggcac tggcggctcc ttttttcaaa ccaaactggg taaggaacgc
     1261 ttgaggaaga aaattgctct cgtaccacga tacatcaaaa tgcaaacacc caaaccagag
     1321 caaaatggct gccgttcggc tgagcccggt tttctgggac agggtttggg caattgcctc
     1381 atcccagggg atccgtccac gttgacgaac cagctcaacc agcgttcgga tgcgttctgt
     1441 cccgctccac cccgtaattg gaacactttc gtgggtaatg gtatggagca acgggttaaa
     1501 ttctccggtt gtcgtcaact ctagagctga gaatgtcatt ttatattcat tcgcgtacca
     1561 ttccctggca accctggtga aataaatgct gccatgttgt tgaaaagtcc aaaaacttcc
     1621 tggtttctgc catttggcat cttcttctcc agaaccggta caggtgcctt taaaaaatcg
     1681 aaactggagg ctgtcatcgg caaagatcgt ggtggcccac ccctcaagaa agtccaggat
     1741 ttcaccgcag tgaacctgat cgccatccag aagggactga tagttcacgg ctgcgacaaa
     1801 ggctggtgcg ccgattttcc ccatcagtcg aatgaaatgg aaagttgacc aggtcaatga
     1861 ccgggtttcc agtttttcca gttcatcctg gtttccaagg taggcggccc gaaaccagtc
     1921 gctgatttcc ccaatatgtt tgccaatgac tttgccctgg ttaggagtgg aatcaatgcc
     1981 aaacggtttc aacgccaaat tcacctttgc atccagtgga aatggaatca gatccaggtt
     2041 ttcagtttca gcgaccggag ctgggttggg aggtgagacc agtttgtgta atttggtgtt
     2101 caacgtcgcg gcttccagga tgatgccggc aaccccgcgt ttcaggtttg agtgtgtcag
     2161 ttccgggagg tattggggga gtttctcgac cagtgagtca agacgctgct ggagatcagc
     2221 ttcagggttg gatacatttt ctttgcccag gtcaaacaac tggtgagcgg tttcatctga
     2281 tacccggcga agggccagtg acccggcttc atcccgcact ttgaagaagt gccaccacac
     2341 aggaggcacg agtttgaatg ttcctcgggc atagctggtg ttggcgcctg atgaatagtt
     2401 gtaggtgccg atgcgtcctg attccgccag gttccagatg gtaacgtcat aatagtgtga
     2461 g
//
LOCUS       DAOQDO010000852         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37864_length_2460_cov_3.531809, whole genome shotgun sequence.
ACCESSION   DAOQDO010000852 DAOQDO010000000
VERSION     DAOQDO010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37864_length_2460_cov_3.531809"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..>2460
                     /locus_tag="PKZ53_13590"
     CDS             387..>2460
                     /locus_tag="PKZ53_13590"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ41520.1"
                     /translation="MNPTNLTRRCLSVFVLVSTLLCLPILLGTSFAASAQTTAGRLVV
                     TVKDPTDAVVAGATVTATNEGTTQSITVTTNEKGVAIFPQLLVGNYTVVAEAEGFQKT
                     QTEGLKIDVGQEYGAVLVLQPGVAENIITVTAGEALVQTTNAELASTVNQKQVQELPL
                     DGRNPLALIQLQAGVNQSGNTTTVINGFRTSTSTLTQDGINIQDNFIRANGLDFTPNR
                     TSVAQTQEFTVITQNPDASIAGSSSVRGVSPSGTNELHGSAFEFHRNDAVGANTFFNN
                     ASKVERPKLIRNQFGFTLSGPVVIPKVVNGKDKLFFFGFYEGFRERAGNPSLITTLLP
                     NARQGVFTYRDNQGQTRTINVLGLRDLSADPFMQNIISRLPGANTIEGGDGFNTAGFR
                     LNQSDFRNRNVGGGRIDYSINASHRLEGVYNYTNEKDARTDIDSINQIPKASTDSNVN
                     FAVGAWNWQVNNNLNNEVRIGGNRSSVNFNTSEDPFDFFTVSNLFTDPIVQFQPQGRT
                     TTILSFIDNAAFTKGDHFIRFGGQFDRTRVRSFNFGGVTPVFGLGFSANSDIDALTSR
                     DFPGGIDAFQLSIANGLLADVAGVLANSARTFNVTSQNSGFVPGAGEVRNFQTRAYSS
                     YITDSWRIHPRLTVNAGLRYDYYTPLREENNLALLPVTNGRSGRETVLDPNGTYDFVG
                     TDLIPIELI"
BASE COUNT          612 a          663 c          618 g          567 t
ORIGIN      
        1 cacgaactaa actccgagtt tcaattctct gaaaatccag ccggcacaaa cggagaaaat
       61 tccgctgtgc tggcttcttt tttctggaaa acccgaaaaa aaagaaatga attggaaaat
      121 ttggattttt tattcagatt tttggaaggt ttccggaaag tgatgtattt gaataacttc
      181 aaaaaactgt ctccggaatt ctaagtattg aattcaaaag ccctcccttc aaaatcggaa
      241 attctggttg taactggcac aggagttgcc gctcccaaag ggatatctat ggccaacaac
      301 cattccggtt ctcatcggtc atatctcaaa gcgaactgaa cacaaaaaaa actgttgaaa
      361 cacctcacac ttgaagggag aagtttatga atccgacgaa tctgacacgc aggtgtctga
      421 gtgtgtttgt gctggtgagt acgctgcttt gtttaccaat tctgttggga acaagctttg
      481 cggcttcggc gcagaccact gcaggccgcc tggtggtaac cgtcaaggat ccaactgacg
      541 cggttgtcgc cggtgcgaca gtaactgcca ccaatgaagg gaccacccaa tcgatcaccg
      601 tcacgaccaa tgaaaagggc gtggcgattt tcccgcagtt gctcgttgga aactacacgg
      661 ttgtcgccga agccgaaggg ttccaaaaaa cgcagacgga aggtctgaaa attgacgttg
      721 gtcaggaata tggcgccgta ttggtgctcc agcccggcgt cgctgaaaac atcatcaccg
      781 tcacggcggg tgaagccttg gtgcagacca ccaatgccga actggcctca acggtgaacc
      841 aaaagcaagt acaggaacta ccgctggatg gtcgcaaccc gctggcgctg atccagttgc
      901 aggctggtgt gaaccagtcc gggaacacca cgaccgtcat caatggcttc cgtacgtcaa
      961 cctcaacctt gacccaggat gggatcaaca ttcaggacaa cttcatccgc gccaacgggt
     1021 tggacttcac acccaaccgg acgagcgtgg cccagacgca ggaattcacc gtcatcaccc
     1081 agaacccgga tgcttcaatc gccggttcat cttcggtgcg tggtgtgagc ccctctggaa
     1141 cgaatgaact ccacggttca gcgtttgaat tccaccgcaa cgatgccgtc ggtgccaaca
     1201 ccttcttcaa caatgcgagc aaagttgaac ggccaaaatt gatccggaat cagtttgggt
     1261 tcacgctcag tggaccagtg gtgattccaa aggtcgtcaa cggcaaagac aagctgttct
     1321 tctttggttt ttatgaaggt ttccgtgaac gggccggcaa ccccagcctg atcaccacat
     1381 tgctgccaaa tgcccgccag ggtgtgttta cctaccgtga caaccagggc cagacccgca
     1441 cgatcaacgt gctcgggctg cgcgatttgt cagccgatcc atttatgcag aacatcatct
     1501 cccgcctgcc tggtgccaat accattgaag gtggagatgg atttaacacg gctgggttcc
     1561 gtctgaatca gtcagatttc cggaatcgca acgtgggcgg cgggcgcatt gactatagca
     1621 tcaacgccag ccatcgtctg gaaggtgtct ataactacac caatgaaaaa gatgcccgga
     1681 ctgacattga cagcattaac cagattccaa aagccagcac cgattccaac gtgaattttg
     1741 cggttggcgc gtggaactgg caggtcaaca acaatctgaa caacgaagtt cgcatcggcg
     1801 gcaaccgcag ttcagtgaat ttcaacacga gcgaagatcc atttgatttc ttcaccgtgt
     1861 caaacctgtt tacggatccg attgttcagt tccagccaca gggccggacc accacgattc
     1921 tcagctttat tgacaacgct gccttcacca agggcgacca cttcatccgc tttggtggtc
     1981 agtttgaccg gacgcgggtt cgctccttca actttggcgg tgtgacccca gtctttgggc
     2041 tcggctttag cgccaattct gacattgacg cgttgacatc acgtgatttc ccaggtggta
     2101 ttgatgcctt ccagttgtca attgccaacg gcttgctggc tgatgtggcc ggtgtgctgg
     2161 ccaactcggc ccggaccttc aatgtgacct cacagaactc aggttttgtg ccaggtgccg
     2221 gcgaagtccg taacttccaa acccgggcgt attcaagcta catcactgac tcgtggcgta
     2281 ttcatccccg tctgaccgtc aatgccggtc tgcggtatga ctactacacg ccgcttcgcg
     2341 aagaaaacaa cctggcgttg ttgccagtga ccaatggacg aagtggcagg gaaacggtgc
     2401 ttgatccaaa tggcacctat gatttcgtgg gcaccgatct cattccgatc gagctgattc
//
LOCUS       DAOQDO010000853         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37898_length_2459_cov_3.437188, whole genome shotgun sequence.
ACCESSION   DAOQDO010000853 DAOQDO010000000
VERSION     DAOQDO010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37898_length_2459_cov_3.437188"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="PKZ53_13595"
     CDS             complement(<1..1614)
                     /locus_tag="PKZ53_13595"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ41521.1"
                     /translation="MRSFQRNLKLLCIGVFLLGWSAVAYAFSSGPPAGRTGAPGELTC
                     TNCHGGSPNTAGGRLSITGLPASYTPGTKYSVTVTVAQNGRQRWGFQITSLTDDGSFA
                     GSFTLTNPSQTQQVSTSVSGKQRTYVQHTSAGTQRGTQNQASFTFDWTAPASNVGAIT
                     FYSSGNAANGDGTSSGDSIYTASVKVNAPAAAQPPTVTTLNPNRGSTVGGTSVTISGT
                     NFANGISATFDGLAAQTTFVDNKTIRVTTPAHAAGAVDVVVKNSDGLSGTLKGGFTYE
                     APQQSPTLTGITPTFGPTTGGTDVTLTGTNFAQGLSVSFGSRELTPVSVSATEIKVRT
                     QPNSAGSVDVIVRNPDGQQAVQANAFTFEGGNPGTTVEVIAPNGNEVLSAGGLPFNIT
                     WNVVTDSTAVQNIELSVDGGATYTTQIATGLASSAMSFAFAVPAGTTSEQGRIRVSVQ
                     QGDAIVSDESDANFRILAAPVISSISPTSAAASSTVKKLIINGSGFQTGAIVEVDGVA
                     TTAKVKVKPTSIQVKKFPAGAAGTRKVRVR"
     gene            2008..2172
                     /locus_tag="PKZ53_13600"
     CDS             2008..2172
                     /locus_tag="PKZ53_13600"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ41522.1"
                     /translation="MHSDNRKGEKFLCRHCGHTADADTVGAQNILARTLATFGSVESP
                     ELQREVSQSV"
BASE COUNT          562 a          613 c          697 g          587 t
ORIGIN      
        1 gcgaacacgc actttccggg tacccgcggc cccggctggg aatttcttga cctgaatcga
       61 agttggcttg actttgactt tggcggttgt tgccacaccg tcaacttcga caatcgcgcc
      121 ggtttggaag ccgctgccat tgatgatgag cttcttcacg gttgaactgg ctgcggcgga
      181 ggttgggctg atggatgaaa tcaccggggc ggccagaatt cggaaattgg catcgctttc
      241 atccgagaca atggcatcgc cctgctgaac cgaaacccgg atgcgtccct gttcggacgt
      301 tgttccggct ggaactgcaa aggcaaacga cattgctgaa gaggccagcc cggttgcgat
      361 ctgagtggtg tatgttgcgc caccatccac tgaaagttca atattttgca cggcggttga
      421 atcagtgacc acattccagg tgatgttaaa tggaaggccg ccagcactca ggacttcatt
      481 gccatttggg gcaatgactt cgaccgttgt gccggggttg ccaccttcaa acgtaaaggc
      541 attggcctgt accgcctgct gcccatctgg attgcggacg atgacatcca cgcttccggc
      601 tgagttgggc tgggttcgaa ccttgatttc ggtcgctgaa acggaaaccg gagtcagttc
      661 gcgcgagcca aatgagacgg acaatccctg ggcaaaatta gttcccgtca aggtgacatc
      721 cgttccgcca gttgttggcc caaaggttgg ggtgattcca gtcaaggttg gtgactgttg
      781 aggtgcttcg taggtaaatc cgcctttgag tgttcctgag agaccatccg aatttttgac
      841 caccacatca acggcgccag cggcgtgagc tggtgtggtc acgcggatgg ttttgttgtc
      901 cacaaaggta gtttgggcgg cgagtccgtc aaacgtggcc gaaataccat tggcgaaatt
      961 ggtacccgaa atagtgactg aggttccgcc cacggttgaa ccacgatttg gattgagtgt
     1021 ggtgacggtc ggtggttggg ccgccgccgg ggcattgacc ttcacactgg ctgtgtaaat
     1081 gctgtctcca gaactggtgc catcgccgtt ggcggcattt ccagatgaat agaaggtaat
     1141 ggcgccaacg ttggaggctg gcgccgtcca gtcaaaggtg aaggatgcct gattttgcgt
     1201 tccacgctga gtcccagcac tggtgtgctg aacataagtg cgttgttttc cactcacgga
     1261 cgtgctgacc tgctgggttt ggctggggtt ggtcagggta aatgacccgg caaatgatcc
     1321 atcatccgtg aggctggtga tctgaaaacc ccagcgttgg cgaccgtttt gagccaccgt
     1381 cacggtcacg gagtatttcg tgccaggtgt ataactggct ggaagtccag taattgacag
     1441 gcggccaccc gctgtgttgg gcgaaccacc atgacagttg gtacaggtca attcgcctgg
     1501 tgctccggtc cgtccggcgg gcgggccaga ggaaaaggca taggccacag ccgaccagcc
     1561 aagaagaaac acaccaatac aaagcagttt tagatttcgc tggaatgagc gcataacaat
     1621 attcaccttt aataaaattg ccctgggggt aatgaggagg tgcgattctg aggttgaatc
     1681 agccccgaag cctaccatta ctctgtccac tagcaaataa atcctgcctg accggttgca
     1741 tatagatgac aagcggtctt taaacatttt ggttgattta tagactaata caatgtaata
     1801 tagtcagtta tgaaccgatc agattgaggt gaatcaacca aacggctcat tcatcacaac
     1861 ctgggatggg ttcacaatat caagcaagtg catcgtggac ctaccggcac ggactcaccc
     1921 ggatctccaa actcgcccaa gtgaaccgtg ttcagcttgc ggcgtcgatc cagccaatcc
     1981 ggcccggact tttcccagtg tggtacggtg cattcggaca accgcaaggg agagaagttt
     2041 ttgtgtcggc attgtggtca caccgccgat gccgataccg tcggcgcaca aaacattctc
     2101 gctcgtacct tggcgacttt cgggagtgta gagtccccgg agcttcaaag ggaggtgagt
     2161 cagtcggtgt aatatctgac tattcatttc tcaactcttg atttagggtt cagggttcag
     2221 ggttttcgga agggatgaga gatgagggat gaaaaaaatc acctttttca cccggccatt
     2281 ttatcacctg tcacctgtta acatatcctg gttttcgccg cgtagcggtg gttgaattga
     2341 ggcaggtcgt ttacggcctg taggaaggac caaaattcga aaaccctgac ccctgacccc
     2401 tgacccctga cccctgaatg tgtcagagac aaaaatggca caaagtggca ggtgaatcc
//
LOCUS       DAOQDO010000854         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37959_length_2457_cov_3.082015, whole genome shotgun sequence.
ACCESSION   DAOQDO010000854 DAOQDO010000000
VERSION     DAOQDO010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37959_length_2457_cov_3.082015"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKZ53_13605"
     CDS             <1..788
                     /locus_tag="PKZ53_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41523.1"
                     /translation="NRPLAEKILAVVADPVFGGPDDDRLNPHIRNLVRPALNLTAQRI
                     LDRIQSHPPAPTSSEAAAETTGQVLIPRLPGTRAEANQIANLLPVQNVLKITDFEATR
                     ELITSNQLSRFQYVHFATHGFLDTARPQLSALVLSMVDKAGTPKDGFLRAFDVYNLNL
                     PAELVVLSACETGLGKSVNGEGLVGLTRGFMYAGTKRVIVSLWSVSDTATSDLMTRFY
                     KKLLVEKQRPAAALRAAQLELMRNTKWKEPFYWAPFIIQGEWK"
     gene            1017..>2457
                     /locus_tag="PKZ53_13610"
     CDS             1017..>2457
                     /locus_tag="PKZ53_13610"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41524.1"
                     /translation="MTLPHHLSQNLILNWLLMWALVPGPFLIFVTRMQQVDQLEPGKL
                     VARELKGGEKHTYPLQLNANDYLKLVVNQNGIDVVVRLIGPDGKMAQEVNSPNGTQGE
                     EPFFYITDHPGTYTLEIESLRKTAQAGSYELKSESVKPATEQDRAEVEIKKLNDELTQ
                     LWQAGKYDQGVLLAQQVVEKSEKCLGRDHSLLADSLNYLGLFYKAKGEYLKAEPLYLR
                     AIAIGEKTIGLQHPHIAAMRNNLANLYAARGDYLKAKDELQRAVAIFEKTLGPEHQNT
                     IFCLNNLAELYVNLGDNTTAERLFRQTLAIREQVFGPDHPTTADTLQNLAFLHRIKGE
                     YIKAEGLYQRVVTIREKASGPEHPETSNALNELAELYRDQGNLPKAESLHQRALAIRE
                     KVFGPDHIAVARSLNNLGLLYRDKGDFGKAEQLLLRSQAIREKKLGMNHPSIAVGFNN
                     LALIAKHKGDYAQAESLYQRALEIQEKAVG"
BASE COUNT          657 a          658 c          608 g          534 t
ORIGIN      
        1 ggaatcggcc actggctgaa aaaatacttg ccgtggtggc ggatccggtc tttggtggac
       61 cagacgacga ccgcctcaat ccgcacatcc gcaacctggt gcgaccagcc ttgaatctaa
      121 ccgctcaacg cattctggac cggattcagt cacacccacc ggcacccaca tcatccgaag
      181 cagcagccga aaccaccgga caggttctga ttccacgctt gcctggcacc cgcgccgaag
      241 ccaaccagat tgccaatctg ctgccagtcc agaatgtgtt gaaaattacc gattttgagg
      301 ccacccgcga actgattacc agcaatcaac tgagccgctt tcagtatgtc cattttgcta
      361 cccacggttt tttggatacc gcgcgccctc aactctcggc gctggttcta tcaatggtag
      421 acaaggctgg aacgcctaaa gatggatttc tacgggcctt tgatgtgtac aacctgaatc
      481 ttccagcgga actggtggtc ctttcggcct gtgaaacggg acttggcaaa tcagtcaacg
      541 gcgaagggct cgttggactc actcgcggct ttatgtatgc cggaaccaaa cgagtgatcg
      601 tcagtctctg gagcgtcagt gataccgcca cttctgatct gatgacccgg ttttacaaga
      661 aactgctggt tgaaaaacaa cgtcccgccg ccgccctccg ggctgcccag cttgaactga
      721 tgcgcaatac aaaatggaaa gagccctttt actgggcgcc gtttatcatt cagggagagt
      781 ggaaatgaag gttcgggatt ttcggaaaga atgaggaatg aaggatgagg gatgagggat
      841 gagggatgag ggatgaaaga aaaccaattc ttcaacccct ggtccaattt cttcagccct
      901 catccccaag ctctcagccc caagccccta gcccttagcc cattttcttc agccccaacc
      961 cctcatcccc aagtcctcat cccgttttct tcaaccctga ttttctggaa ttcaccatga
     1021 cacttcccca ccatttgagc caaaacctga ttttaaactg gttgctgatg tgggcactgg
     1081 tacctggtcc ttttttgatc tttgtgacca gaatgcaaca ggttgaccag ttggaacctg
     1141 gaaaactggt tgcacgcgaa ttgaaaggcg gcgaaaaaca cacctaccca ctccagctca
     1201 atgccaacga ttacctgaaa ctggtggtga accagaacgg cattgatgtc gtggtgcggc
     1261 tcattgggcc ggatggaaag atggctcaag aagtaaatag ccccaatggc actcagggtg
     1321 aagaaccatt tttctacatc actgaccacc ccggcactta tacactcgaa attgaatcac
     1381 tgagaaaaac ggcccaggct ggcagctatg aactgaaatc agagtccgtc aaacctgcca
     1441 cggaacagga ccgtgccgaa gtggaaatca aaaaactgaa tgacgaattg acccaactct
     1501 ggcaagccgg aaaatacgat caaggggtgc tcctggcaca gcaggtggtg gaaaagagcg
     1561 aaaaatgctt aggcagagac cattccctgc tggctgacag cctcaactat ctcggtctgt
     1621 tttataaagc caaaggcgag tatttgaaag ccgaaccgct gtatttgcgt gcgatagcaa
     1681 ttggtgaaaa aacaatcggc ctccaacatc ctcacatcgc ggccatgcgc aacaacctgg
     1741 caaacctcta tgccgccagg ggggactatc tgaaggccaa agatgaactt cagcgcgcgg
     1801 tagccatttt tgagaaaaca cttgggcctg aacatcaaaa cacgatcttt tgcctcaaca
     1861 atctggctga gctgtatgta aacctgggcg ataacaccac tgcggaacgt ctgtttcggc
     1921 agacattggc cattcgggaa caagtgttcg gtccagatca tccaacaaca gcagatacac
     1981 tccaaaatct ggcgtttctt catcgcatca aaggcgaata tatcaaggca gaagggttgt
     2041 atcagcgagt ggtgacgatt cgtgaaaaag catctggccc ggaacaccct gaaacatcca
     2101 atgccctgaa tgagttggcc gagttgtacc gggaccaggg caatctgccc aaagcagagt
     2161 cgctgcacca acgcgctttg gccattcgtg aaaaagtatt tggccctgat cacatcgccg
     2221 tggctcgcag cctgaataat ctcggcttgc tctatcgtga caaaggggat ttcggaaagg
     2281 cggaacaact cctgctgcga tctcaggcaa tccgcgagaa gaaattgggg atgaatcatc
     2341 cttcgatagc cgtgggattc aataacctgg cgttgatagc caagcacaag ggtgattatg
     2401 cgcaagctga atcgttgtac cagcgtgccc tggaaatcca ggaaaaagcg gttggtc
//
LOCUS       DAOQDO010000855         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_37961_length_2457_cov_2.934638, whole genome shotgun sequence.
ACCESSION   DAOQDO010000855 DAOQDO010000000
VERSION     DAOQDO010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37961_length_2457_cov_2.934638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2431)
                     /locus_tag="PKZ53_13615"
     CDS             complement(<1..2431)
                     /locus_tag="PKZ53_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ41525.1"
                     /translation="MISSSQPLAPSPRFYKTGDRVRWLPDGNVEFLGRLDDQVKVRGH
                     RIELGEIETILQQHPEVQAAAVRVLKTGQPDAFLVAYVTTRSESNIGQAGVETELRAY
                     LKGQVPAFMVPSVFVHLDQMPLTPSGKIDRHRLPAPESVGSQETGERIAPRTATETLV
                     AELFAELLFQPKIGIHHNFFELGGHSLLATRLATRVREAFAIEFPLQQVFSHPTVAEL
                     AGAIDLERAQTQVRASAPPLQTVSRNQKLPLSFTQQRLWFLDQLEPGSAAYIMPAALR
                     LTGELNLEALRNAFSELVKRHEILRTTFVTTDGEPALQISELARFTFAIEDASTRSID
                     LADTQSATIQQFKTEMLQFLWRPFDLAAGPLLRVQIVKLAKTDHLLLISMHHIVSDGW
                     SIGVMLREVAALYRAGVTGQPSSLPALPVQFADYAAWQRDFLNSERLEAELAYWKQKF
                     AAPLPVLSLPTDRPRPEVQTFQGSDETFSLSPELSEKVRALSRQHGSTLFMTLLAAFQ
                     TLLLRYSGQEDIVVGSPIANRNHLETEGLIGPLINTLALRTDFSGQPTFIDVLNRVRQ
                     TTLEAFAHSEVPFEKIVDAVQPERDMSRTPLFQVMFDFGNQARNRQKGQTLPGLSITP
                     VEFESRIARLDLTLSMVDTGHSLAGNLEFNTDLFDRVTIRRMLGHFQTLLENLVANPE
                     QPVTTVQLMTATELHQILVEWNDTQLEYPKDKCLHQLFEAQAVRAPLATAVVFGDAEL
                     TYGELNARADALAGYLCSLGVGPDTLAGICVERSLEMVVGLLGILKAGGAYVPLDPTY
                     PLERLIFMIQDA"
BASE COUNT          581 a          660 c          677 g          539 t
ORIGIN      
        1 gggcatcctg gatcataaag atcagacgtt ccagcggata ggttgggtca agcggaacgt
       61 aggcgccgcc agctttgaga attcccagca gcccgaccac catttccagc gaccgttcca
      121 cacagattcc agccagcgta tctggcccaa cacccagcga gcacaggtat ccggccagcg
      181 catcggcccg ggcattgagt tcgccatagg tcagttctgc atcgccaaag acaacggcgg
      241 tggccagtgg cgcccgcact gcctgggctt caaacaactg gtgcaggcat ttgtcttttg
      301 gatactcaag ctgcgtgtca ttccattcaa ccagaatctg atggagttcc gtcgcggtca
      361 tcaactgaac ggttgtcacc ggctgttccg gattggcgac cagattttcc agcagagttt
      421 ggaaatggcc aagcattcgc cgaatcgtca cacggtcaaa caagtcagtg ttaaattcga
      481 ggttcccggc cagtgagtgg ccggtatcca ccatcgaaag cgttaaatca aggcgggcaa
      541 tccggctttc aaactccact ggtgtgatcg agagtcccgg caacgtctgg cctttttgac
      601 gatttcgggc ctgatttcca aagtcaaaca tgacctgaaa cagtggtgtc cggctcatat
      661 cgcgttcggg ttgaacggca tccacgattt tttcaaaggg aacttcgctg tgggcaaagg
      721 cttcgagcgt ggtctggcga acgcggttga ggacgtctat aaacgtcggc tggcctgaaa
      781 aatcagttcg gagtgccagg gtgttgatca acggaccaat taacccttcc gtttccaggt
      841 ggttgcggtt ggcaatcggt gacccaacca caatgtcttc ctgcccggag taccgtaaaa
      901 gcaaggtctg aaaagccgcc agcagcgtca taaacagcgt gctgccatgc tgacgactca
      961 gcgcacgcac tttctcggaa agttcaggcg acaggctgaa agtttcatcc gaaccctgga
     1021 aggtctgtac ttcgggccgt ggccgatctg tcggaagcga taaaaccgga agcggagccg
     1081 caaacttttg tttccagtag gcaagttctg cttccagccg ttccgaattt aaaaagtcac
     1141 gttgccaggc ggcataatca gcaaattgaa ctggcaacgc cggcagcgat gatggttgcc
     1201 cggtcacgcc agcccgatag agtgctgcca cttcgcgcaa catcacaccg attgaccatc
     1261 cgtctgaaac aatgtggtgc attgaaatca gcagcaggtg gtcggttttg gccagtttca
     1321 ctatctggac tcgcagcagc ggcccggcag caaggtcaaa cgggcgccac aaaaattgca
     1381 gcatttcggt tttaaactgt tgaatggtcg ctgattgcgt atccgccaga tcaatcgagc
     1441 gggttgacgc gtcttcgatt gcaaaggtaa acctggctaa ttcagagatc tggagtgctg
     1501 gttcaccatc agtggtaaca aacgttgtgc gcaggatttc atggcgcttg accagttctg
     1561 aaaatgcgtt tcggagggct tccagattga gttcgccagt caatctgagg gccgcaggca
     1621 taatataggc cgcgctgcct ggttcaagct gatccaggaa ccagagccgt tgctgagtga
     1681 aggaaagcgg caatttttga ttgcgggaaa cagtctgtaa cggtggcgca ctggctcgaa
     1741 cctgggtttg cgcgcgctca agatcaatcg ctccggccag ttcagccacg gttggatgcg
     1801 aaaaaacctg ctgtaatgga aattcaatcg caaatgcttc gcgcacacgg gttgccagcc
     1861 gcgtggccag cagggaatgc ccgccaagct caaagaaatt gtggtgaatg ccaatctttg
     1921 gctggaagag aagctcggca aagagttcgg ccaccagggt ttcggttgct gttcgcggag
     1981 caatcctttc gccagtttct tgtgaaccca cactttctgg cgctggcagg cgatggcgat
     2041 caattttccc gcttggagtc aacggcattt gatcaagatg aacgaaaacc gacggcacca
     2101 taaaggctgg tacctgacct ttgagatagg ctcggagttc agtctcgacc ccagcttgac
     2161 caatgttcga ctcagaccgg gtggtcacat aggccaccag aaacgcatct ggctgaccag
     2221 ttttcagtac ccggacggcg gcggcctgaa cctcagggtg ctgctgcaaa atggtttcaa
     2281 tttcgcccaa ctcgatacgg tgtccacgca ccttgacctg atcatccagc cgtccaagaa
     2341 attcgacatt tccatccggg agccagcgga cacggtcccc agttttgtaa aaccgagggc
     2401 ttggggctaa gggctgagag ctagaaatca tcaatgattg aaccccccca gccccca
//
LOCUS       DAOQDO010000856         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38061_length_2453_cov_2.350292, whole genome shotgun sequence.
ACCESSION   DAOQDO010000856 DAOQDO010000000
VERSION     DAOQDO010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38061_length_2453_cov_2.350292"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PKZ53_13620"
     CDS             complement(<1..1673)
                     /locus_tag="PKZ53_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41526.1"
                     /translation="MEVLLNGKRTKLNPAQSIGKGGEADVFNIGQNRALKIFKNASHP
                     DYHGLPHEQQAAQERLAVHQEKLKAFPRSLPARIVKPEDLATDLSGKQIVGYSMKFLA
                     GGELLLRYSDRTFRQAGVTNESVVSIFKDLHTTVGDLHRSGVVIGDFNDLNVMVLGTE
                     ANLIDADSFQFGKFFCQVFTQRFVDPLLCDPQAQSLMLFRPHTADSDWYAFAVMLMQC
                     LLFVDPYGGIYKPKGGSKPAVPHDLRPLHRITVFNPAVRYPKPAIPYKVLPDELLQYF
                     HLVFEKDSRGQFPADLLDTLRWTTCTACNTEHARMVCPNCAQATPAAVKSVTVIRGTV
                     TATQLFTTRGRILAVEYQNGDLRWLVHENGTYRREDGGTVLHGGLDPKMHFGLCGNRT
                     LLARDGLLLTLTPDNPQIDRLSVDMYHNQPAVAATENARYWVTSGQLMRDGSYGPELV
                     GEVLTGQTQFWVGSKFGFGFYRAGELSVAFTFHTAQPGLNDNVTLPKFRGQLLEANCL
                     FTDDRAWFFTSENIQGRTVNRCTVIRPNGTIEATTEAEAGDGSWLSTLRG"
     gene            complement(1789..>2453)
                     /locus_tag="PKZ53_13625"
     CDS             complement(1789..>2453)
                     /locus_tag="PKZ53_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41527.1"
                     /translation="TQAILKQTEPHEAEALSTAIMAGDFWHTVREATLSQLQTVATAL
                     GGSLSQVVSDYLLFTVVGVIVTPLKTVIFSLGDGVYAINGDVKTIGPFPDNMPPYLAY
                     GLVNSSLATSNPERLHFCIEAVRPTAEVQSVLIATDGLEELLRSTGCKLPGKNDVVGA
                     VSQFWEADHYCSNPDAIRRKLSLMNAESRRPDWENRRLVKEPGLLSDDTTLVVIRKRQ
                     TS"
BASE COUNT          586 a          658 c          629 g          580 t
ORIGIN      
        1 ccgcgaagcg tcgaaagcca gcttccgtca ccggcttcgg cttccgttgt ggcttcaatg
       61 gttccgttgg gccggatgac cgtgcagcgg ttgacagtgc ggccctgaat gttttctgaa
      121 gtgaaaaacc atgcccggtc atcagtgaac aggcagttgg cttccagcaa ttgtccgcga
      181 aatttcggca gagtgacatt gtcgttgagt cctggttggg cagtgtgaaa agtgaaggcg
      241 acactcagtt caccggcccg ataaaaacca aaaccgaact ttgacccaac ccaaaactgg
      301 gtttgcccgg tgaggacttc gccgaccagt tccgggccat agctgccatc gcgcatcaac
      361 tgcccactgg taacccagta ccgggcattt tcggtggccg cgactgctgg ctggttgtgg
      421 tacatatcca cgctcaaccg atcaatctgt ggattatcgg gggtcagtgt cagcaataat
      481 ccatctcggg cgagcaaggt gcggttaccg cacagaccaa aatgcatttt gggatccagc
      541 cctccgtgta gcaccgtgcc accatcttcc cggcgatagg tgccgttttc gtgaaccagc
      601 cagcgcaggt caccattctg gtattcaacc gctaaaatcc ggccccgcgt tgtaaacaat
      661 tgggtggccg tgacagtccc acgaatgaca gttactgatt tcacggctgc cggcgtggcc
      721 tgggcacagt ttggacagac catccgggcg tgttcggtgt tgcaagccgt gcaggttgtc
      781 cagcgtaacg tatcaagcag gtcagccggg aactgaccac gcgaatcttt ttcaaagacc
      841 aggtgaaaat actgtaacaa ctcatctggc aataccttat aagggatggc cggtttggga
      901 taccgcaccg ccgggttaaa caccgtgatc cgatgcaagg gtcgcaaatc gtgcggaacg
      961 gctggtttgc ttccgccctt gggtttatag atgccaccat acggatcaac aaacaacaaa
     1021 cactgcatca gcatgaccgc gaaggcatac cagtctgaat cagccgtgtg cggacgaaat
     1081 aacatcaggc tttgtgcctg tgggtcgcac agcagcgggt ctacgaatcg ctgagtgaac
     1141 acctgacaga aaaatttgcc aaactgaaag ctgtcggcgt caatcaaatt ggcttctgtt
     1201 ccaagcacca tcacgttgag gtcattaaag tcgccaatca caacgcctga gcgatgcaaa
     1261 tcacccaccg tggtgtgtaa atctttgaaa atactgacca ctgattcatt ggtgacacca
     1321 gcctggcgga aagtgcggtc actatagcgc agcaacaatt cgcctccggc cagaaatttc
     1381 atcgaataac caacgatttg ctttccactc aggtcagtgg ccagatcttc cggtttgaca
     1441 attcgggctg gtaacgaacg tggaaaggct ttgagttttt cctgatgaac tgccagtcgt
     1501 tcctgggcgg cctgttgttc gtgaggtagc ccgtgatagt cagggtgcga agcgttttta
     1561 aaaatcttga gtgctcgatt ttgcccaata ttgaacacat cagcttcgcc acctttacca
     1621 attgactggg ccggattcaa tttggttcgc tttccattta agaggacttc catactgcct
     1681 cacttccact gcctcagatc ttcttttcaa atcgtcgctt tgcatcaacc agaagcgaaa
     1741 gatgaaaaag ttgtatttcc ctgaaccctg aaccctgaac cctgaaccct aactggtttg
     1801 tcgtttccga atcaccacca gcgttgtgtc atcagagagc aggccgggtt ctttcaccag
     1861 gcgccggttt tcccaatctg gacggcggct ttcagcattc atcagcgaca gtttgcggcg
     1921 gatggcatct gggtttgaac aatagtggtc agcttcccaa aactgactga cggcgccaac
     1981 gacatcattt tttccaggca atttacagcc agtcgaacgc agcaattctt cgaggccatc
     2041 ggttgcgatc aacactgatt ggacttcagc cgttggtctg accgcttcaa tgcagaagtg
     2101 cagccgttct gggttagaag ttgccagcga cgaattgacc agcccatagg ccaggtacgg
     2161 aggcatattg tcaggaaacg ggccgatggt ttttacatcg ccattgatgg catacacgcc
     2221 gtctccgagg gaaaaaatca cggttttgag cggtgtcacg atgacgccga cgacggtaaa
     2281 caacaaataa tcactcacga cttgagacaa tgacccaccc agggccgtcg ccacagtttg
     2341 aagctgactc aaagttgctt cccgaacggt atgccagaaa tcccctgcca taatcgccgt
     2401 gctcaacgct tcggcttcgt gaggttcggt ttgtttgaga atcgcctgcg tta
//
LOCUS       DAOQDO010000857         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38130_length_2450_cov_3.958664, whole genome shotgun sequence.
ACCESSION   DAOQDO010000857 DAOQDO010000000
VERSION     DAOQDO010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38130_length_2450_cov_3.958664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKZ53_13630"
     CDS             <1..378
                     /locus_tag="PKZ53_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41528.1"
                     /translation="PSPAAVIPVETPMFLRSYVDPDQQADEVFSERFVMATVTPYMLF
                     TFGVLSKELGFPRSIGALLAQLAFGLFLVLPVVGIMALGKGSDQRKSQRNLFFGTLIA
                     SGPLIFLFYLIFVAILFGGSCGN"
     gene            383..793
                     /locus_tag="PKZ53_13635"
     CDS             383..793
                     /locus_tag="PKZ53_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41529.1"
                     /translation="MPVTSMVKGSPYTTGMNLARHVHLGLALWPWIWLVLLGMLLWYS
                     SHLLGHWPVPYRDDPFLIGSGWFYQGLVGSVMTGFFTSVIYVPSWPIVTIVLAPMLTR
                     QEYKRRWGLFIFGVVAVVGCLGMNSGYLLNWLSD"
     gene            925..1566
                     /locus_tag="PKZ53_13640"
     CDS             925..1566
                     /locus_tag="PKZ53_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41530.1"
                     /translation="MLKYKWNHGVVTFIVSLLASLIVPWITDDPACRPFGLGLALFLM
                     FVVAGVEYWPPGVSDRFWMTWIFFTMMSVWAIVAWWGFPPGGLAVLVLLVLAAFFRDC
                     VVWTKIKWWVWLTVFLGMQLVARPVFDDILNCINHLPGAESFARPYWRPFTEVPFSFF
                     HGAELMKMRRRGGPLRPEELAVLNIALVGLFIAGCWKPVRNWLHLNSDPAHHS"
     gene            1618..2157
                     /locus_tag="PKZ53_13645"
     CDS             1618..2157
                     /locus_tag="PKZ53_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41531.1"
                     /translation="MAAHPGFSIRQETLARRCEICHQSDSFIPETNHCSRCDGVPTAP
                     EPSPAAVIPVETPMFLRSYADPDQQAYEIFSERFVMAAVTPYMLFTLGIISREVRFPD
                     EVVGLLIILAAALFFILPLIGMATLIDLYKKGKSIRTLFLGTLIASGPMIWVLLSVFL
                     SDFFGMGGTGTQTINCAGG"
     gene            2267..>2450
                     /locus_tag="PKZ53_13650"
     CDS             2267..>2450
                     /locus_tag="PKZ53_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41532.1"
                     /translation="MAAHPGFSIRQETLARRCEICHQSDSFIPETNHCSRCDGVPTAP
                     EPSPAAVIPVETPMFLR"
BASE COUNT          535 a          537 c          638 g          740 t
ORIGIN      
        1 ccatcacctg cggcagtgat accggttgaa actccaatgt ttctcaggag ttatgttgac
       61 ccagaccagc aggcagacga agttttctct gagcgttttg tcatggccac agtaactccc
      121 tatatgcttt tcacgtttgg ggttctgagt aaggagcttg ggtttccccg aagtatcgga
      181 gcacttctgg cccaactggc gtttgggtta tttttggttt tgccagttgt tggaattatg
      241 gctctcggaa aaggatctga tcaaagaaag agtcagcgga acttattttt tgggacactt
      301 attgcttcag gcccgctgat attccttttt tatctgatat ttgtggcgat tttgtttggg
      361 ggcagttgtg gaaattaatg aaatgcctgt cacctccatg gtgaagggat cgccatacac
      421 caccggaatg aaccttgccc ggcatgtcca tcttggtctt gcgctttggc cgtggatctg
      481 gttggtattg ttggggatgt tactctggta cagctcacat ttgttgggac actggcccgt
      541 gccgtatcgt gatgatccat ttctgattgg gtcagggtgg ttttatcaag gactggtggg
      601 aagtgtgatg acgggatttt ttacgtcggt gatttatgtc ccgtcgtggc caattgtgac
      661 gattgttctg gctccaatgt tgacccgcca ggaatacaaa cggcgctggg ggctcttcat
      721 ttttggcgtc gtggctgtgg ttggatgcct gggaatgaac tctggatatt tgttgaactg
      781 gctgagtgat taataccaat tgagaatgaa gaatgaagaa tgatttaaac tcagttattt
      841 cagatattta atgatttcct ggtacaagaa gttcattcca gacatagtat aagaacccat
      901 gtgatgaaag gcagtaaccc tgcaatgctg aaatacaaat ggaatcacgg ggtagtcacg
      961 ttcatcgtca gcctcctggc ttccctgatt gttccgtgga taaccgatga tccggcctgt
     1021 cggccatttg ggcttgggct tgcgctgttt ctgatgtttg tggtggccgg agtcgaatac
     1081 tggccgcccg gtgtatcgga tcgtttctgg atgacatgga tctttttcac catgatgagt
     1141 gtctgggcca ttgtggcctg gtggggattt ccgccgggag gtctggctgt tctggttttg
     1201 ctggttctgg cggctttttt tcgcgattgt gtggtgtgga caaagatcaa atggtgggtc
     1261 tggcttactg tttttctggg aatgcaactt gtcgctcgac cggtatttga tgacatcttg
     1321 aattgtatca atcatctacc cggtgctgag tcctttgcgc gtccgtattg gaggccgttt
     1381 acagaagtcc ctttttcatt tttccacggc gcggaattga tgaaaatgcg acgcagaggc
     1441 ggcccacttc ggcccgaaga gctggctgtt ctcaatatcg cattggttgg gcttttcatc
     1501 gctggttgct ggaagccggt tcgaaactgg ttgcatctca attcagatcc ggctcaccat
     1561 tcataaaaca cctggataca aagggtttct accctcaatc aagaaaggtt actgcttatg
     1621 gcagcgcatc ccggtttttc aatcaggcaa gaaactctgg cgcgacggtg tgaaatttgc
     1681 catcagtcag acagctttat cccagaaacc aaccattgct cccggtgtga tggagttcca
     1741 accgcgccag agccatcacc tgcggcagtg ataccggttg aaactccaat gtttctcagg
     1801 agttatgccg acccggacca gcaggcgtat gaaatcttct ctgagcgttt tgtcatggcg
     1861 gcagtaactc cctatatgct tttcacgctt gggattatca gcagggaagt cagatttcca
     1921 gacgaagtgg ttggattgct tataatactg gcagccgcct tattttttat tttgccatta
     1981 attggaatgg ctaccctcat tgacctttac aaaaagggga aaagcattcg aacactcttt
     2041 cttgggactt tgattgccag tgggccaatg atctgggttt tgctttctgt ttttttgtca
     2101 gatttctttg gaatgggggg aactggtact caaaccatta attgtgctgg tggttaattt
     2161 gctctagttc tcactgaaga ggattacctg ttcgaatcca actcaccatt cataaaacac
     2221 ctggatacaa agggtttcta ccctcaatca agaaaggtta ctgcttatgg cagcgcatcc
     2281 cggtttttca atcaggcaag aaactctggc gcgacggtgt gaaatttgcc atcagtcaga
     2341 cagctttatc ccagaaacca accattgctc ccggtgtgat ggagttccaa ccgcgccaga
     2401 gccatcacct gcggcagtga taccggttga aactccaatg tttctcagga
//
LOCUS       DAOQDO010000858         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38146_length_2450_cov_2.540710, whole genome shotgun sequence.
ACCESSION   DAOQDO010000858 DAOQDO010000000
VERSION     DAOQDO010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38146_length_2450_cov_2.540710"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1153)
                     /locus_tag="PKZ53_13655"
     CDS             complement(2..1153)
                     /locus_tag="PKZ53_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNJ41533.1"
                     /translation="MKTKAAVLYEMGRSQPFAHSLPMVIDELTLAGPESGEVLVEIVG
                     AGLCHSDLSVVDGSRPRIMPMVMGHEASGIVREVGPEVTEFAPGDHVVFSFVPVCGHC
                     LYCASGRGALCEFGARANIAGTLLSGGCRFTDSESRRCYHHLGVSAFSQFTVAAQESL
                     VKIDPSVSLEMAALFGCAVMTGVGAVVNTARVEPGTSAAVFGLGGVGLSVVMGLRAIG
                     AYPIIAVDRVDDKLTLAHELGATHTVNAGQDDPVVAIKEITRGGAAYAFESVGAETVL
                     IQAYQATGRGGTTITIGLPHPGKQFSISAVSLTLEERTVKGSYMGSSVPRRDIPRFIN
                     MYQAGILPVDKLHTHTLKLDEINLGFDRLAQAQAVRQIVTFNQSVVSSQ"
     gene            1489..2259
                     /locus_tag="PKZ53_13660"
     CDS             1489..2259
                     /locus_tag="PKZ53_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNJ41534.1"
                     /translation="MFSKAIVRKPGATFAQGITTSTAGTPDLEMALTQHQRYREALHA
                     CGLSLTVLEADDQFPDSTFVEDTAILTPYFAVISRPGASSRQGEAQRMAEVLADHIHT
                     LYAITAPGTVDGGDICQVGHHFFIGISERTNEAGAKQLAEFLRLAGYSSSTIDIRPIP
                     GLLHLKTGLSYLEHQTVLVTRAMLSCHQFAGFKVICVPEDEEYAANCVYVNGRVLIPA
                     GYPKVAALLTEARYQPVPLEMSEFQKMDGGLSCLSLRF"
BASE COUNT          590 a          648 c          614 g          598 t
ORIGIN      
        1 actactgact actgactact gactgattga aagtaacgat ttggcggaca gcctgcgcct
       61 gggccagccg gtcaaatcca aggttgattt cgtcgagttt caaggtgtgg gtgtgcagtt
      121 tgtcaaccgg caggattcca gcctgataca tattgataaa ccggggaata tcgcggcgcg
      181 ggacggatga gcccatgtaa gaacctttga ccgtccgttc ttcaagcgtc aggctgactg
      241 ccgaaatcga gaattgttta cctgggtgtg gaagcccaat cgtgatcgtg gttccaccac
      301 gcccggttgc ctgatatgcc tggatcaaca ccgtttcggc gcccacgctt tcaaacgcat
      361 aggcggcacc gccacgggtt atttctttaa ttgccaccac tggatcatcc tgaccggcgt
      421 tgaccgtatg ggttgcccca agctcatgcg ccagtgtcag tttgtcatct acgcgatcaa
      481 ccgcaatgat gggataggcg ccaatagctc tgagccccat cacgacactg agcccaacgc
      541 cgcccaatcc aaacacggca gccgatgttc caggctcaac ccgggctgtg ttgaccacgg
      601 caccgacgcc ggtcatcacg gcacatccaa aaagggctgc catttcgagt gaaacggaag
      661 ggtcaatttt caccagtgat tcctgggcgg caacagtaaa ctgtgaaaag gctgagaccc
      721 caaggtggtg ataacaccgg cgagattccg aatcggtaaa ccggcagcct cctgagagaa
      781 gtgtcccggc gatgttggcc cgggcaccaa attcacacag cgcccctcgt cccgaagcac
      841 aatagaggca gtggccgcag accggaacaa aactaaacac cacatggtct cccggcgcaa
      901 attcggtgac ttccggccca acttcgcgga caatcccgct ggcttcgtgg cccatcacca
      961 tcggcataat gcgtggccgc gagccatcca cgactgacag atccgaatgg cacaacccgg
     1021 cgccaacaat ttcaaccagg acttcgcctg attcaggccc agccagggtc agttcatcaa
     1081 tcaccatcgg cagactgtgg gcaaaaggct gtgaccggcc catttcatag agaacagcag
     1141 cttttgtttt catggttttc cttgtgattc aaaaaagaag atgtcggctg gcttttcaat
     1201 tgcaaatcag gggttggaac aaatagcatt ttgagggagt caaactcaaa ttgaatacca
     1261 gttagtagcc agtagtcagt agtcagtagt tcgctaagtt tatttgattg aattacttag
     1321 ttgttgacaa atataaaagt ttcattgcga attggtaagc accctgtcct aagtttcctg
     1381 agcgattgaa tttggtaagt gcttgatttt ttccgtgttt tccgtgtttt ccgtggttaa
     1441 aatgcctgaa aattttcggt aaggtactta taactctaag gatcaattat gttttcaaaa
     1501 gcaattgttc gcaaaccagg ggccaccttc gcccagggaa ttaccacttc aaccgccggc
     1561 acacccgatc ttgagatggc gttgactcaa catcagcggt atcgcgaagc attgcacgcc
     1621 tgtggcctgt cgttgacggt attggaagca gatgaccagt ttcctgattc gacgtttgtt
     1681 gaagatacag caattctcac gccgtacttt gctgtcatct ctcgccctgg cgccagcagc
     1741 cggcagggtg aagctcagag aatggcagaa gttctggccg accacatcca taccctgtat
     1801 gccatcactg cccctggaac ggttgacgga ggcgatattt gccaggtcgg ccaccatttt
     1861 ttcattggta tttccgaacg taccaacgaa gccggggcga aacaactggc cgaatttctg
     1921 cggctggctg gctatagttc ttcgacgatt gatattcggc caatccccgg cctgttgcat
     1981 ttgaaaacgg ggctttccta tctggaacac cagaccgtgc ttgtcactcg ggccatgctg
     2041 agctgtcatc aattcgctgg atttaaagtg atttgtgtgc cggaagacga ggaatatgcc
     2101 gccaactgtg tgtatgtcaa cggtcgagtg ctcattcccg ccggataccc caaagtggca
     2161 gcactcttaa ccgaggccag ataccagcct gtaccgcttg aaatgtcaga atttcaaaaa
     2221 atggacggtg gattgagttg tttgtcgctc cgattttaga actgatctgg ggagacactc
     2281 cctgagtttg ttcagcttcg cacaagccaa atcccaaaag cggacagttt ctcgccgggt
     2341 caccgctttg ttctatttcc tttctctttg accgtgttga agcctgatcc aaacctgtcg
     2401 cttataccag cttgcagtga acctttcgta tcagcaagga gtcaaatagt
//
LOCUS       DAOQDO010000859         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38151_length_2450_cov_2.425470, whole genome shotgun sequence.
ACCESSION   DAOQDO010000859 DAOQDO010000000
VERSION     DAOQDO010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38151_length_2450_cov_2.425470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="PKZ53_13665"
     CDS             <1..1843
                     /locus_tag="PKZ53_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41535.1"
                     /translation="QPAGWKSRDGKLWFPTIKGLVEIHPTQIRRNEIAPPVVIEQLQF
                     GTAQLPLNASPELSWDKETLEIRYAGLSFLAPERVRFRFRLEGYNHNWIEADTRRVAY
                     YTSLPPGKYTFQVIACNNDGVWNEQGATYRFSVKTPLSQTWWAYLIYATGLILIGYVV
                     VRYRTIQLQRSNDLLESRIRARTRELAELVEHFKTAEQTALALRDKAMTSEVKALEAS
                     RAKSMFLATMSHEIRTPMNGIIGMSYLLLDSKLTRDQREQAETIQNSANSLLTILNDI
                     LDFSKIEAGKLEFEVIDFDLRKLIKDIVELLGKLATDKGLTLTTIIAPDVPTQCSGAP
                     TRIRQVLTNLLGNAVKFTEHGTVSLNVKAIEKNLARTSLRFEITDTGIGIDPAIQRHI
                     FDPFSQADESMTRRFGGTGLGLAICKQLVEMMGGEIGVDSALGQGACFWFTVQLTAHS
                     AQEHHHPSPAAPALAPQQVVAVPPADPKNLLFERPVLLVEDNVVNQKVVCRQLEKLGL
                     KITIANNGKEAVEATRHTAFALILMDCHMPEMDGFEATTKIRQQEGPDRHTPIIALTA
                     GALQTDADKCYQAGMDDFLAKPFKPEELRHVLQRWLNPLECGGLTPL"
     gene            1875..>2450
                     /locus_tag="PKZ53_13670"
     CDS             1875..>2450
                     /locus_tag="PKZ53_13670"
                     /inference="COORDINATES: protein motif:HMM:NF022970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNJ41536.1"
                     /translation="MKNKSVPFSIGNGVIVKKGVKDPDSGVKMDGWQGRVINIQDTNP
                     KEILLVIAWDSLTLQNLPESYIKESEEQGLDWSEMCLYPTDVEPAVARDTPRDVERTV
                     SEIRSNAHWYGLGDEGARIQSVLENVNPWDGRGALDAWYEHLEENLSFPFEAEITEHQ
                     SRGRLRLGDKVKVLDIIDQDDMYGILVAVKQG"
BASE COUNT          645 a          664 c          616 g          525 t
ORIGIN      
        1 acaaccagcc ggatggaaaa gtcgggacgg caaactctgg tttccgacca tcaagggcct
       61 ggttgagatt catccaaccc aaattcgacg caatgaaatc gccccgccag tcgtaatcga
      121 acaacttcaa tttggcacag ctcaactgcc gctcaatgcc tcaccagaac tctcctggga
      181 taaagaaacg cttgaaattc gctatgcggg cctgagtttt ttggcgccag agcgggtccg
      241 gtttcgcttt cggcttgaag ggtataatca caactggatt gaggccgata cccgacgggt
      301 ggcctattac accagtttgc cgcctggaaa gtatacattc caggtcatcg cgtgtaacaa
      361 cgatggtgtc tggaacgaac agggcgccac ctatcggttt tcagtcaaaa ccccactttc
      421 ccaaacctgg tgggcctatc tgatttatgc aacgggcctg attttgattg gatatgtcgt
      481 ggtcaggtac cgaaccattc agctccaacg aagtaatgat ttgcttgaaa gtcggatccg
      541 agcccgaacc cgtgaactgg ccgaactggt cgagcatttt aaaactgccg aacaaaccgc
      601 tctcgccttg cgcgataaag ccatgacctc cgaggtaaaa gcactcgaag ccagccgggc
      661 caaaagtatg tttctggcca ccatgagcca cgaaatccga accccgatga atggcattat
      721 cggcatgtcg tatctgctgc ttgattcaaa gttaaccaga gaccagcgcg aacaggctga
      781 aaccatccag aactcagcca attccctgtt gacgatcctc aatgacattc tggatttttc
      841 caaaatcgaa gctggcaaac tggaatttga agtcattgac tttgacctgc gtaaactgat
      901 caaggatatc gtcgaacttc tagggaaact ggccaccgac aaagggctca ccttaaccac
      961 catcattgcc ccggatgttc caacccagtg tagcggggca ccaacccgga ttcgccaggt
     1021 tctgaccaat ctgctcggca atgccgtgaa atttaccgaa cacggcacag tgtcactcaa
     1081 cgtgaaggcc attgaaaaaa atctggctcg aaccagtctc cgatttgaaa ttacagatac
     1141 cggaatcggc attgacccgg cgattcagcg gcacattttc gatccttttt cacaggcgga
     1201 tgaatctatg acccgtcggt ttggcggtac gggtctgggg ctggcgattt gcaagcaact
     1261 cgtggaaatg atgggcggtg aaatcggcgt tgacagcgcg ctggggcagg gtgcctgctt
     1321 ctggtttacg gtccaactga cagcacattc ggctcaggaa catcaccacc catcacctgc
     1381 tgcgccagcg cttgcacctc aacaggttgt tgccgtgccc ccggctgatc ccaaaaatct
     1441 tttgttcgaa cgcccggtgt tactcgtcga agataacgtc gtgaaccaaa aagttgtatg
     1501 ccggcaactt gaaaaacttg gattgaaaat caccattgcc aacaatggca aggaagcagt
     1561 tgaagccacc cgccacacgg catttgcctt aatcctgatg gattgccata tgcccgaaat
     1621 ggacgggttt gaagccacca ccaaaatccg ccagcaggaa ggccctgacc gccatacacc
     1681 gatcattgcc ctgaccgctg gcgcgttgca gaccgatgcc gacaaatgct atcaggccgg
     1741 gatggatgat ttcctagcca aaccattcaa accggaagag ttgcgccacg tcctccagcg
     1801 atggctaaac cccctggagt gcggcggctt gacgccgctt tagacttcca ccccaaacaa
     1861 agcgagaaaa cagtgtgaaa aataaaagtg tccccttctc catcggcaac ggtgtcattg
     1921 tcaaaaaagg tgtcaaagac ccggattccg gtgtcaagat ggacggctgg caaggccggg
     1981 tgatcaacat tcaggacacc aacccgaaag aaatcctgct ggtcattgcc tgggatagcc
     2041 tcacgctcca aaacctgccg gagtcttata tcaaggaaag cgaagaacaa gggcttgact
     2101 ggtccgagat gtgcctttat ccaactgacg tcgaaccggc tgttgctcgc gatacaccca
     2161 gggacgttga gcgaacggtt tcagaaatca gatcgaatgc ccactggtac ggccttggtg
     2221 acgaaggcgc ccggattcaa tcagtattag aaaatgtcaa tccgtgggat ggacgaggtg
     2281 ccctggatgc ctggtatgaa cacctcgaag aaaacctgtc ctttcccttt gaagctgaaa
     2341 ttaccgaaca tcagagccgg ggacggcttc gccttggcga caaagtgaaa gtcctcgaca
     2401 tcattgatca ggatgacatg tatggaatcc tggtagcggt caaacagggg
//
LOCUS       DAOQDO010000860         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38173_length_2449_cov_2.993317, whole genome shotgun sequence.
ACCESSION   DAOQDO010000860 DAOQDO010000000
VERSION     DAOQDO010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38173_length_2449_cov_2.993317"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PKZ53_13675"
     CDS             complement(<1..1152)
                     /locus_tag="PKZ53_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41537.1"
                     /translation="MTRIFKLGIVAILFVLGIGTSQAYGQQIVTDPELNQQLITFQQN
                     FPQFVSDHPGLTSGASDINIEGAQKKVSFSFQQQGQYNVTYGNLVFSPTTPISPRRFL
                     MSLDIQSITHTGSAAIDITDKKGRNFNMQCQDNSFAVGAVKFTSEVIVPESGTVVILN
                     RQVNFDPKTVVVSIPCLKARQTPDELTADAEFDDSWQEDLGQGLIDTALAERGSSPSK
                     GTLNTVNATMQALAAVPGSSVATSTTEAVTTNGTMTAASAPAPCNGTVQADIQLVITD
                     LSAISAQAQAQGFQTAVQTLTTDLRTIIPTLTAPSQSTVQKFIDDLNKYTASTSMGGT
                     TITAIEQAVLINDFNNVVLSTGITSTQLTTLQTDLVGVLNTLSGISTAQL"
     gene            1404..2384
                     /gene="meaB"
                     /locus_tag="PKZ53_13680"
     CDS             1404..2384
                     /gene="meaB"
                     /locus_tag="PKZ53_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNJ41538.1"
                     /translation="MSTAHTLAEGILKGNIRSIARGITAVENNTPDALSLMKQIFPHT
                     GKTTVIGLTGSPGAGKSSLVDTLAKLYRQRGEKVGILAVDPSSPFSGGAILGDRIRMQ
                     SLALDPGIYIRSMATRGNLGGLARATSDAALILDAAGYDRVIVETVGVGQDEVDIVKT
                     ADVTLVVMVPGMGDDIQSIKAGIMEIGDLFIINKSDREGVERIEKEIEAMLSLGHRPD
                     FWQPPILKTVATRHQGLDEVIEAIASYLTFLQQHQQARQDRQARIAELKLTELLRDKL
                     LSAARRTAATGTEWSTWASQIATRERDPYSIVEEILDRLNLSGDPKLLSK"
BASE COUNT          573 a          632 c          673 g          571 t
ORIGIN      
        1 aagctgggcg gttgaaattc cagacagggt gttcaggact ccgaccagat cagtttgcag
       61 cgtggtaagc tgggttgagg taatcccagt gctcagcacg acattgttga aatcattgat
      121 caaaaccgcc tgctcaatgg ccgtgatggt ggtgccgccc atgctggtcg aagccgtgta
      181 tttgttgagg tcatcaataa acttctgaac ggttgactgg ctcggtgcgg tcagggttgg
      241 aatgatggtt cgcaaatcag tggtcagcgt ttgaacggct gtttgaaatc cctgggcctg
      301 agcctgagct gaaatcgctg acaggtcggt aatcaccagt tgaatgtcag cctggacagt
      361 accgttacag ggggctggcg ctgacgcagc cgtcatcgtg ccattggtgg tcacggcttc
      421 tgtggttgag gtggcaacgc ttgaaccagg caccgccgcc agggcctgca tggtggcgtt
      481 gacggtattg agcgttcctt tgctggggct gcttccccgt tcagccagcg ccgtgtcaat
      541 cagcccctgc cccaggtctt cctgccacga atcatcaaac tcagcgtcgg cagtcaactc
      601 atctggcgtt tgcctggctt tcagacacgg gattgagacc accacggtct tcggatcaaa
      661 attgacctga cggttgagga tcaccacggt gccagactcg ggaacgatga cttcggaagt
      721 aaatttaacc gctcccacag caaaagaatt gtcctggcac tgcatgttaa agtttcttcc
      781 ctttttatca gtgatatcaa tcgcggcgct gcccgtgtgg gtaatgctct gaatatcgag
      841 cgacatcaga aaccgacgtg gactgatcgg ggtcgtgggg ctgaaaacca ggttgccata
      901 ggtgacattg tactggccct gctgttgaaa gctaaaggag actttctttt gagcgccttc
      961 gatgttgatg tctgaagcgc cggaggtcaa tcctggatgg tcagaaacaa attgcgggaa
     1021 gttttgctga aaagtgataa gctgctggtt taattccggg tcggtcacaa tctgttgccc
     1081 atatgcctga ctggtcccga taccaagaac gaacaaaata gcgacaatac caagtttgaa
     1141 aatacgtgtc atatagcttc tccttgtgcg atctgaactt attttgatga agtcttgttt
     1201 acaggaacgc actgtgccag agacgcgatg tgattcaaaa aacaacccgc ccactgacgg
     1261 ttttttgagg gttacagacg aatccaggtc actgagccgc aggaccaagc cggtcagata
     1321 cgtccggcag gacttgaatg ccggcgctgg attcctagaa tacagttctt cgtttttact
     1381 ttctcccgaa ggagtttcag gttttgagta ccgcacacac gctggctgaa ggcatcttaa
     1441 agggcaacat ccgctctatc gcccgcggca ttaccgccgt tgaaaataac acccccgatg
     1501 cactgtcgtt gatgaaacag atttttccac acaccgggaa aaccaccgtg attgggttga
     1561 cggggtcacc cggtgccggc aagagttccc tggttgatac actggccaaa ctctatcgcc
     1621 agcgcggcga aaaggtcggt atcctggcag ttgacccgtc cagcccgttt tcaggtggtg
     1681 ctatccttgg tgatcggatt cgaatgcaat ctctggccct ggatccaggg atttacatcc
     1741 ggagtatggc cacccgaggc aacctcggcg gtctggcccg ggcaacctct gacgccgcat
     1801 tgattttgga tgccgccggg tatgatcggg tgattgtcga aaccgtcggc gtcgggcagg
     1861 acgaggtgga tattgtcaaa accgccgatg tgacgctggt ggtgatggtg cctggaatgg
     1921 gcgacgatat ccaatctatc aaagctggga ttatggaaat cggcgacctg tttatcatta
     1981 acaaatctga ccgcgaaggc gtcgaacgga ttgaaaagga aatcgaggcc atgctcagtc
     2041 ttggccaccg ccctgatttc tggcaacctc cgatcctcaa gaccgtggcc acgcgccatc
     2101 agggtcttga cgaagtaatt gaggctattg caagctatct gactttcctg caacaacatc
     2161 aacaggcacg gcaggaccga caggcacgga ttgccgaact caagctcacc gaactgctgc
     2221 gagacaaact cctctcggct gcccgtcgca cagcggcgac gggtactgaa tggtcaacct
     2281 gggccagcca gatcgccact cgcgaacgcg acccatactc gattgttgag gaaatccttg
     2341 accgtctcaa cctctcaggt gaccccaaac tactctcgaa atgaagggat atgggcaaac
     2401 aacctatgaa aattgaccat ttgggaattg cagtcgaatc cctggaaac
//
LOCUS       DAOQDO010000861         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38199_length_2448_cov_2.975345, whole genome shotgun sequence.
ACCESSION   DAOQDO010000861 DAOQDO010000000
VERSION     DAOQDO010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38199_length_2448_cov_2.975345"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>2448)
                     /locus_tag="PKZ53_13685"
     CDS             complement(107..>2448)
                     /locus_tag="PKZ53_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ41539.1"
                     /translation="QSVVLFGQTEVPAKINVSRGKIVVRNAPGSFFQNSGAIDIRVAD
                     AQGNQSNAVRLVVGSGNSIRVDQPESMVVNVQNKVTIKATVLDVNGSPIPNSTLSFQS
                     ANPTQASVDATGQVTGLAAGAATIQITSGDAVRTVTFSVNGVSVVDSGIFGDGDILVV
                     PSLGRIFASDLRNHVIKGAQVGQPLANLAGSPGNPGLSDGPFTASRFSGPLGLGLGTQ
                     RIFLADTANQAIRRFNLQTNQVDTIIEVSDLASAGVTSWGPRGVVQATDGNLYITDAQ
                     NSVIWQARLLGTQVQMSVLAGGIGQPGLVDGAATGARFNNLQEIAITGNILTVTDRGN
                     KVVRLVALPSGAVSTIGRSNAGRPDQTTRLNTIPQADVVFDDPEGVDVDTAGNIYVTD
                     GATVKVLSIANNQINSITDLAQPGTFQKAVGVSVGTDSTFVLDSGKGQVIRVGTSAPT
                     ITSISPTQINAASPPEIVVVGTNFLTETSVTVGPIPAASVLVESSTRLRFTLPSLPFG
                     GTLQVVVQHRGGSAAGSLTVVGTPSAPANLVAKVISPTEVTLTWQDTSSNETGFVVER
                     KLGESGSFVQVSTTQPNTTSFSDRNLTQRISVTYRVRAVNTIGASANSNEATAVPRNL
                     VQITFSPRASTLSPGESLSTGVNFEVSTEPGDQPGTILLDIPLDPNRFDLAKATATPG
                     GLIPSEFYAPSDLVVAPFENGIRIILTSSRNPFNAQISGGKGIFCTISVPVLAGAPSG
                     SVSIDVSAEPPFATQFNESLKDGAKIIPFSSTPAPITIR"
BASE COUNT          593 a          633 c          653 g          569 t
ORIGIN      
        1 acctggactg aaaagaacga cgcagcatac caaatcctca tcaaaaggaa tgataaatta
       61 tgaatcacaa aaccagttca taattcataa ttcatcattg gttttattac cgaatggtga
      121 tcggtgccgg ggtactggaa aatggaatga ttttggcccc gtccttgagc gattcgttga
      181 attgagtcgc gaacggtggc tcagccgaaa cgtcaattga aactgagccg gaaggtgctc
      241 ccgcaagcac tggaacagaa atggtgcaaa agatcccttt tccaccactg atctgagcgt
      301 tgaacggatt gcgactgctg gtgagaatga tccgaatccc attttcaaac ggggccacca
      361 ccagatctga tggcgcgtaa aactctgaag ggatgagccc accaggggtg gcggtggcct
      421 tggcgagatc aaaccggttt ggatcaaggg ggatatccag caagatagtg cctggctggt
      481 ctccaggttc agtacttact tcaaagttca caccggtgga aagcgactca cctggtgaca
      541 gggttgatgc ccgaggtgaa aacgtgattt gaaccagatt tcgtggcacc gccgtggctt
      601 cgtttgaatt ggctgaggcg ccgatagtat ttaccgcccg gacgcgatag gtgaccgaaa
      661 tacgttgggt taaatttcga tctgaaaaag aagtggtatt gggttgagtg gtactgacct
      721 ggacaaatga accgctctca ccaagttttc gctcaaccac aaatccggtt tcgttggaac
      781 ttgtatcctg ccaggttaag gtgacttcgg tcggtgagat cactttggcg accagattag
      841 ctggtgccga tggggtcccg actacggtca gggaaccggc tgccgagccg ccgcgatgtt
      901 gaaccacgac ctggagcgtt ccaccaaacg ggagtgacgg gagggtaaat cgcaggcggg
      961 ttgaactctc aaccaaaacc gaagctgccg gaatcgggcc gacggtgact gaagtttcgg
     1021 tcagaaaatt tgtgcccacg accacaattt ctggtgggga agcggcgttg atttgggttg
     1081 gtgaaatgct ggtaatggtc ggagcgctgg tgcccacccg gatcacctga cctttgccag
     1141 aatccagcac aaacgttgag tccgtgccga ccgacactcc gaccgccttc tggaacgtac
     1201 cgggttgagc cagatctgta atcgaattaa tttgattatt tgcaatggat aagactttga
     1261 cggtggcccc gtcggtgaca taaatattac cggcggtatc cacatccaca ccctctggat
     1321 catcaaacac cacgtcagcc tggggaatcg tgttgagccg tgtggtctga tctggacgtc
     1381 cggcatttga ccttccaatg gtgctgactg cgccacttgg gagggccacc agtcgcacca
     1441 ccttgttacc gcgatctgtc acggtcaaaa tattgcctgt aatcgcaatt tcctgcagat
     1501 tgttaaatcg agcgccggtg gccgcaccat caaccagccc tggctggccg attcctccgg
     1561 ccagaacact catttggacc tgggttccca gcaatcgcgc ttgccagata acactatttt
     1621 gagcgtcggt aatgtataaa ttcccgtctg ttgcctggac gactccgcgt ggcccccagc
     1681 ttgtgactcc ggcactggcc aaatctgaga cttcaatgat ggtatcaact tgattggttt
     1741 gaagattaaa ccgccgaatg gcctgattgg ccgtgtccgc cagaaagatt cgctgcgtgc
     1801 ccagtcccag tccgaggggc ccgctaaacc tggatgcggt aaatgggcca tctgataatc
     1861 ccggatttcc tggtgagcca gccaggttgg ccagcggctg gccaacctgg gcccctttaa
     1921 tgacgtggtt gcgcaaatca ctggcgaaaa tccggcctaa actgggaaca accagaatat
     1981 ctccgtcacc aaagatccct gaatcaacca ctgagacacc gttgaccgaa aatgtaacgg
     2041 ttcgaaccgc atcgcccgag gtaatttgaa tcgtggcggc cccggcagcc agaccagtca
     2101 cctgtccggt tgcatcaact gaagcctggg ttggattcgc gctttgaaaa ctcagtgttg
     2161 aattgggaat cgggcttccg ttgacatcca aaacggttgc cttgatggtg actttgtttt
     2221 gaacattgac caccatcgac tcaggttgat caacgcggat tgaatttccg ctccccacga
     2281 ccagtcgaac cgcatttgat tgattgcctt gagcatcggc tactcgaatg tcaattgccc
     2341 cgctattttg aaaaaatgaa cccggcgcat ttcgaacgac aattttaccc cgactgacat
     2401 tgatcttggc cgggacttca gtctgaccaa agagcaccac tgattgag
//
LOCUS       DAOQDO010000862         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38219_length_2447_cov_2.841973, whole genome shotgun sequence.
ACCESSION   DAOQDO010000862 DAOQDO010000000
VERSION     DAOQDO010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38219_length_2447_cov_2.841973"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1794)
                     /locus_tag="PKZ53_13690"
     CDS             complement(190..1794)
                     /locus_tag="PKZ53_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ41540.1"
                     /translation="MAHFQVNNQSFPTRCEICHQADQFDPESNRCHRCAPLQAPKPRP
                     WIEKMMFPATRRELWAWKLMLVTTGFVLIDSLVCSLYPLRAGVLGFLVYRLLDWDFSV
                     SYLGAIYDERMCDWGRIHVEYLFLLLTNGITSLSLFAMNICSFLDFWSHPYKRLHFIN
                     LTQTPHQPGTEKKYHTLKMWRIPAFCLAAGGFLNLIVYGWLVFPTYTLGDAISYGYQP
                     HIELKLLFNKNLHQKCQFGWTPLLYATAFRDQAMMQRLIQRGASVDAPSSTGLTPLMV
                     AAALRSEELCSFLIDQGADTNRADTYGSTPIDFALKQTYLFDEPFSLSFSGAFSVSND
                     TPDQLNQTIALLRRHGARISGHTARRGKTTAQLAAENGLLELVKASVREDPTQINQPD
                     LNGETLLMSAAYKQNVDLVRFLIDQGADVNARTRFHYAVVHFATPNANSAEGEKTSIA
                     IFQLLVQHGADLNAQGYSGDTALFYAAGGNHPRLLKFLLDHGADPTIRNKANRSAVDE
                     AWHPAIHSDHTESIQILETAIAARSK"
     gene            complement(1815..>2447)
                     /locus_tag="PKZ53_13695"
     CDS             complement(1815..>2447)
                     /locus_tag="PKZ53_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ41541.1"
                     /translation="TNSRGETPLWISASRHCPDLCQLFLDAGARVDHPDFKGYSPLLA
                     ALDEYCYNYYITDHRPLDNPIKQRQKHQTVMTLVRAGAAVTGFQTPDRKTIADLAAEA
                     GEYEIFKLAISQKPSEFDQISFTGNSLLFKAIENDDLELVQLLLDKGLMINLENSAGK
                     SPLEIACQSHKPHAKEITSVLLDHGAVFFPVVAHEKPRWSPPFVSTFPQG"
BASE COUNT          646 a          562 c          621 g          618 t
ORIGIN      
        1 aagtcagtag tcagtagttc actcagttta tttgattgaa ttacctgact gtcttcagat
       61 acgaacactt cattgcaaaa tggtatggat cattttggac taaagccctc gcattataga
      121 aaagctaaaa tattgaaaat attgtatttc cctcagccct tagcccgata ccctcagccc
      181 aaattggtat tacttgctcc gagcagcgat tgctgtttcc aggatttgga ttgattcggt
      241 atggtcggaa tgtattgcgg gatgccaggc ttcatcaacg gctgaacggt ttgccttatt
      301 ccgaatcgtc gggtctgccc cgtgatccag caaaaatttc aacaaccggg ggtggttacc
      361 tccggcagca taaaacaacg ccgtatcacc tgaatacccc tgagcattga gatcagcacc
      421 gtgctgaacc agtaattgaa atatggcgat ggaggttttt tctccttcag cgctgtttgc
      481 attcggagtt gcaaaatgca cgacggcata gtgaaatcgg gtccgagcat tcacatcagc
      541 accttgatca attaagaatc tgaccagatc aacgttttgt ttataagccg cagacatcaa
      601 gagggtttct ccgttcaggt cgggctggtt gatttgggtg ggatcttcac gcactgacgc
      661 tttcaccagt tcgagtaacc cgttttcagc ggcgagctgg gccgtagttt tgccccttcg
      721 agctgtgtgc cctgaaattc gggcaccgtg tcggcgcaac aatgcaatcg tttggttcaa
      781 ctggtcaggg gtatcattgg aaacagagaa cgccccggaa aacgagagtg aaaaaggttc
      841 atcgaagaga tatgtctgtt tcaaggcaaa atcaattgga gtcgaaccat aggtgtcagc
      901 ccgattggta tcggcaccct gatcaatcaa aaatgaacac aactcctcgc ttcgcagggc
      961 agccgcgacc atcaaaggcg tcaatccagt gctggaaggc gcgtccacac tggcgccgcg
     1021 ctgaatcaac cgttgcatca tggcctgatc tcgaaaggcc gttgcataaa ggagcggtgt
     1081 ccagccaaac tggcatttct gatgtaagtt tttgttgaat aaaagtttaa gttcaatatg
     1141 aggctggtat ccatagctga ttgcatctcc taatgtatag gttgggaaaa caagccatcc
     1201 gtatacaatg aggttaagga agccaccagc cgcaagacag aacgccggga ttcgccacat
     1261 tttgagagtg tgatatttct tttcggtgcc cggttgatgt ggagtctggg ttagattgat
     1321 gaagtggagt cgtttataag ggtgagacca aaagtcgagg aatgaacaga tgttcattgc
     1381 aaaaagggaa aggctggtaa ttccattggt cagcagcaaa aacaaatatt caacgtgaat
     1441 tcttccccaa tcacacattc gctcgtcata gatcgccccc agataagaaa cagaaaagtc
     1501 ccaatccaaa agccgataga ccaaaaatcc aaggacgccc gcccgaagcg gatagaggga
     1561 gcacaccagc gaatcaatca gcacaaatcc agtcgtcaca agcatcagtt tccaggccca
     1621 gagttcccgc cgcgtggctg gaaacatcat tttctcgatc caggggcgtg gctttggtgc
     1681 ctgtaacggg gcacaccggt gacagcgatt ggactccggg tcaaattgat ccgcctgatg
     1741 gcagatttca caccgggttg gaaaagattg gttgttgacc tggaaatgtg ccattacgtc
     1801 ctctatctca tcgctcaacc ttgagggaag gttgaaacaa atggtggaga ccagcggggt
     1861 ttttcatgag ccacgactgg aaaaaaaact gctccgtggt cgagtaaaac gctggtgatt
     1921 tctttcgcgt gtggtttgtg gctctggcaa gcaatttcca gcggagattt gccggctgaa
     1981 ttctccaaat tgatcatcag gcctttatca agcaggagtt gaaccagctc caggtcatcg
     2041 ttttcgatag ctttgaacag cagggagttt ccagtaaatg aaatctggtc aaattccgat
     2101 ggcttttggg aaatagccag tttgaaaatc tcatattcgc ctgcctcagc cgccaaatca
     2161 gcaattgttt ttctatcagg ggtttggaag ccagttactg ccgctcccgc ccgcaccagc
     2221 gtcatcacgg tttggtgttt ttggcgctgt ttgattgggt tatcaagagg ccggtggtca
     2281 gtgatgtagt agttgtaaca gtactcgtca agtgccgcca gtaacggtga atatccttta
     2341 aagtcaggat gatcaacccg ggcaccggca tcaagaaaaa gttgacacaa gtctgggcaa
     2401 tgtcgtgagg ctgaaatcca taagggggtt tctccacgcg agttagt
//
LOCUS       DAOQDO010000863         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38283_length_2444_cov_2.822101, whole genome shotgun sequence.
ACCESSION   DAOQDO010000863 DAOQDO010000000
VERSION     DAOQDO010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38283_length_2444_cov_2.822101"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKZ53_13700"
     CDS             <1..380
                     /locus_tag="PKZ53_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41542.1"
                     /translation="KTQFIQLEICARRAEFSTKEQLRSLADSHLRNIVQAHQTNRLIN
                     LQLAQAIHQTIQVVLDTWEAKAEFHRWWLGGAIFYFAEVADDEPDFDSPLGFEDDTEV
                     LNACLKFAHCENLCLNPEDYDDV"
     gene            373..>2444
                     /locus_tag="PKZ53_13705"
     CDS             373..>2444
                     /locus_tag="PKZ53_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019953191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ41543.1"
                     /translation="MSEKAMFSPGMRVLCRDAEWLVTKVEVSDTSHSHFAVHCIGTDD
                     LVRGHESVFLTQLDHLEPVDPLKTRLVSDTSSGFRMSRLVLEAHLRQMPITGFEPDLS
                     GMGVFEPMKFQVQTVQRALEQLRPRLLLADSVGLGKTIQVGMILSELMRRGRADRILV
                     LAKKSMLAQFQSELWNRFALPLVRLDSEGIARLRLRIPANRNPFEVYHRIIISMDTLK
                     NVGSYRHFLEATRWDCVVIDEAHNVAGASVPERHLGYRLARLLSRRTDSLLLTTATPH
                     NGKRETFGRLISLLSLEAIPDPTLRQYSADDIKGYFFMRFKEDVREEAGENLTDRLVV
                     PRSQTTAQATVEEERVYAILAEFRRVAHNYRHSDQPWEHRMLLQYGLYKQFLSSPESC
                     RQTVVKRIKSLQNQPVTPELGFLRRLEKALESLSLQASSRYQVLKQQLKTIGWDGSTE
                     SPRVLVFTEYRETQDALAEALNKDFNLNYSKEFGDQPTNVLATIHGSLPDTHLMKTVE
                     GFGTGSSKMRMLLATDVASEGINLHHQCHLIIHYDLPWSIITLIQRNGRIDRFGQKQK
                     PVLRYLMVKTEQDIFRGDDVIFDRLIAKVEEINRSTRQGETVLKLYDPEAEERYIAES
                     GLLVGNTDVLESPPGVSDTEAAELELLLNAANLAGQDDFLKFLLGEETPSAVQVSPSK
                     STPSPTTRL"
BASE COUNT          613 a          602 c          605 g          624 t
ORIGIN      
        1 ccaaaacaca gtttatccaa ttggaaatct gtgcccgccg ggctgagttt tcaaccaagg
       61 aacaactccg ttcgctcgca gactcacatt tgaggaacat tgtccaggct caccaaacca
      121 atcggctgat caatcttcaa ttagctcagg caatccacca aacaattcaa gttgtgttgg
      181 atacctggga agccaaggct gaattccatc gctggtggct gggtggggcc attttctatt
      241 ttgctgaggt tgcagatgat gagccagatt tcgattcgcc acttggattt gaagacgaca
      301 cagaagtact caatgcctgc ctgaaattcg cccattgcga aaatctatgc ctgaaccccg
      361 aggattatga tgatgtctga aaaggcgatg ttctctcccg gtatgcgggt gctttgccgt
      421 gatgctgagt ggctggtaac caaagttgaa gtctctgaca cctctcattc ccattttgcg
      481 gttcattgca ttggcactga tgatctggtg cgtgggcatg aatcggtctt tctgacccag
      541 cttgaccacc tcgaaccagt tgacccactg aaaaccaggc tggtgagtga tacctcaagc
      601 gggtttcgca tgtctcgact ggtgcttgag gcacatcttc gccaaatgcc aataactgga
      661 tttgaacctg atctctcagg gatgggtgtg tttgaaccga tgaagtttca ggttcaaacc
      721 gttcaacgtg ctcttgagca gctccgacct cgattgttgc tggcagattc ggtcgggctg
      781 gggaaaacca tccaggttgg aatgattttg agcgagctaa tgcgtcgtgg gcgggcagac
      841 cgaattttgg tcctggcgaa aaaatccatg cttgcccaat ttcaatcaga actttggaat
      901 cggtttgccc tgcctttggt gcggcttgat tcggaaggca ttgcccggtt gcgtctgcga
      961 attccagcca atcgaaaccc ttttgaggtg taccatcgca tcatcatcag tatggatact
     1021 ttgaaaaatg ttggtagcta tcggcatttt ctcgaagcaa cccggtggga ttgcgttgta
     1081 attgatgaag cccacaacgt ggcaggggcc tctgttcctg agcgacatct tgggtatcgg
     1141 ctggcacgat tgttgtctcg tcgaaccgac agtttgcttt tgacaaccgc cacgccacac
     1201 aatgggaaac gcgaaacgtt tggaagatta atttccttat tgagcctcga agccatccca
     1261 gacccaactt tgcgccagta cagtgcagac gacatcaaag gatatttctt tatgcggttc
     1321 aaagaggatg ttcgcgaaga agctggggaa aatctgactg accggctggt ggtacctcgt
     1381 tcccaaacta cagcacaggc cactgttgag gaagagcggg tttatgccat tttggctgaa
     1441 ttccgtcgtg ttgctcacaa ttatcgccat tcagatcagc cgtgggaaca tcgaatgctg
     1501 ctgcaatatg gcctctataa acagttttta tcaagcccag agtcatgtcg gcaaacagtt
     1561 gtaaaacgca tcaaatcact gcaaaatcag ccggttacac cagagttagg gtttctcagg
     1621 cgtctcgaaa aagcgctgga atcactttca ttgcaggcat catcacgcta ccaggttttg
     1681 aaacaacagt tgaaaacaat tggatgggat ggctcgactg aaagcccccg cgtgcttgtt
     1741 tttactgaat atcgtgaaac tcaagatgca ctcgccgaag cactgaataa agattttaac
     1801 ctcaattata gcaaggaatt cggagaccaa cccaccaacg ttctggccac aatccatggg
     1861 tcgttgcctg atacccattt aatgaaaacg gttgaaggat ttggaaccgg cagctctaag
     1921 atgcggatgc tgctggccac cgatgttgcc agcgaaggca tcaacctgca ccatcaatgt
     1981 caccttatca tccattatga cttgccctgg tctattatca ccttgattca gcgcaatgga
     2041 cgcattgacc ggtttggcca gaaacaaaaa ccggtattgc gatatttgat ggtcaaaaca
     2101 gaacaggata tctttcgcgg cgatgacgtg atttttgatc gcctgattgc caaagttgag
     2161 gaaattaacc gctcgacccg acagggcgaa accgtcctca aactctatga tcctgaggct
     2221 gaggaacggt atatcgctga atctggatta cttgtgggaa atactgatgt cctcgaaagc
     2281 cctccgggtg tcagcgacac cgaagcggct gagcttgagt tgttgttgaa cgcagccaat
     2341 ctggccggcc aggacgattt tctaaagttt ttgcttggtg aagagacgcc ctcagcggtc
     2401 caggtctccc cgtcaaagtc caccccatcc ccaacaaccc ggct
//
LOCUS       DAOQDO010000864         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38343_length_2442_cov_2.872225, whole genome shotgun sequence.
ACCESSION   DAOQDO010000864 DAOQDO010000000
VERSION     DAOQDO010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38343_length_2442_cov_2.872225"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="PKZ53_13710"
     CDS             complement(<1..2019)
                     /locus_tag="PKZ53_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ41544.1"
                     /translation="MILLYPIWLILIIPLAVAWWNWKFPTRLLQGLRAAISLLIVLSL
                     CGLALKLPSQAGTVVIVADRSLSMPLETEAVQKEAIDLIQAARRSNDQVAVVSFGQKS
                     AVEQLPQGGRFGGFVTELGKDASNLNDGLEKALSLIPPDSPGKIVVLSDGKWTGKNPA
                     NAAAIAASRNVAIDYRAISRTSANDVAITQLDAPARVAPGEAFMLTVWVESPTPQELT
                     FELMNGAQVIGSGKREVSSGLNRLTFRDIAKEPGTQNYTVRVQGATQDPIPENNAARV
                     LVGVEGAKPLLCVTQNPASAYAQLLKSGGLNLKVLTPGECSWQLEELAKYSAVLLENV
                     PAQEIGLNGMENLAAWVKESGAGLMMTGGKNAFGPGGYFQSPIDPILPVSMELRREHR
                     KLTLAIVVTMDRSGSMAAPAGGGRIKMDLANLGAVQVYDLLSPFDEFGVIAVDSAPHT
                     IAELGPVDKTSRVRNDILRVDSQGGGIFIYEALSASANMLLKAKAGTRHIILFADAAD
                     SEEPGKYQDLLEQCKQANITVSVIGLGKPSDVDAKLLEDIAKRGNGRIYFTENADDLP
                     RLFAQDTFVVARSTFLEDPTTVKFTGGMVSVTGKQFPGTQLGGYNLCYLRPGATLGSV
                     TVDQYQAPIVATWQAGIGRVVAYTGEADGKFTGAIAGWPNVGDMFSSLA"
     gene            complement(2016..>2442)
                     /locus_tag="PKZ53_13715"
     CDS             complement(2016..>2442)
                     /locus_tag="PKZ53_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41545.1"
                     /translation="RAAQSPGVHQSTCRLGTDAAVTVDPETVSLEIVTPQKQTQSVIV
                     RDKTVFLPANQVGVFELRPKQKTSSGSPPHWYFAVNALSQTESNLMNCVTGQWGSWLK
                     SPGGLTEYRSIAWVCLLLAAAGLVLHSALIARNAQGHRA"
BASE COUNT          571 a          680 c          641 g          550 t
ORIGIN      
        1 tgccagactt gaaaacatgt cgcccacgtt tggccagccg gcaatcgccc cagtaaattt
       61 gccatcggct tcaccggtat aggccaccac ccggccaatt ccagcctgcc acgtggccac
      121 aatcggcgcc tggtattgat caaccgtgac gctccccagc gtggcgcccg gtcggaggta
      181 gcacaggtta tagcctccga gttgggtgcc tggaaattgt tttccagtca ccgaaaccat
      241 tccaccagtg aatttgacag tggttgggtc ttcgagaaag gtacttcggg ccacaacaaa
      301 agtgtcctgg gcaaagagtc gcggcagatc atcagcattt tccgtgaaat aaatccgccc
      361 gttgccacgt ttggcgatgt cttcgagcaa tttggcatct acgtcgcttg gtttccccag
      421 accaatcacg ctgaccgtga tatttgcctg tttgcattgc tcaagcaggt cctgatattt
      481 cccaggttct tcggagtcag ccgcatcggc aaacaaaatg atgtgccggg taccggcttt
      541 ggctttgagc agcatattgg ctgaggccga aagcgcctcg taaataaata tgccgccgcc
      601 ctgtgagtca acgcggagaa tgtcatttcg gacacgtgac gttttatcaa ccggacccag
      661 ttcagcaatc gtgtgtggcg cgctatcaac tgcaatcacc ccaaattcgt caaaaggtga
      721 caacaggtca tagacctgaa cggcacctaa attcgccaga tccattttaa tccggccacc
      781 accggctggc gccgccatac tgccagagcg gtccatcgta accacaatgg ccagggtcaa
      841 tttgcgatgt tcgcgccgca actccattga aaccggcaga atgggatcaa ttggcgactg
      901 aaaatagcct ccggggccaa acgcgttttt cccacccgtc atcatcagcc cggcgccaga
      961 ttccttgacc caggcagcca ggttttccat gccgttgagg ccaatctctt gagctggaac
     1021 attttcgagc agcactgctg aatatttcgc cagttcttcc aactgccagg aacattcgcc
     1081 aggggtcaaa acttttagat ttaagccccc agatttgagt aattgggcat aggcagaagc
     1141 tggattttgg gtgacgcaca gcaatggttt ggcgccttcg actccgacca gaacacgggc
     1201 ggcattattt tccgggattg ggtcctgggt cgcgccctga acccggaccg tataattttg
     1261 agtgccaggt tctttggcaa tatcacgaaa ggtcagccgg ttcaggccag atgaaacttc
     1321 gcgcttgccc gagccaatga cctgggcacc attcatcaat tcaaaggtta actcctgtgg
     1381 cgtcggagat tcgacccaca ccgtcagcat aaaggcttca cccggagcga ctctggctgg
     1441 ggcgtcgagc tgggtaattg ccacatcatt ggcggatgtc cgcgaaatgg cccggtaatc
     1501 aatcgcgaca ttgcgtgaag ccgcaatcgc agcagcgttc gccgggttct tgccggtcca
     1561 ttttccatct gagagcacta caattttgcc gggcgaatca ggcggaatca gcgagagtgc
     1621 tttttccaga ccatcgttga gatttgaggc atcttttcca agttcggtga caaaaccgcc
     1681 aaatcgcccg ccttgcggca gttgttcaac ggctgatttt tgtccaaatg aaacaacggc
     1741 cacctggtcg tttgaccgtc gagccgcctg aatcaggtca attgcttctt tttgaacagc
     1801 ctcagtttca agcggcattg atagactgcg gtcggcaacg attacaaccg taccggcctg
     1861 actgggcaac ttcaacgcca ggccacacag gctcagcaca atcaacaggc tgatagctgc
     1921 ccgcagtccc tggagtaacc gggttggaaa cttccagttc caccaggcaa cggccagcgg
     1981 aatgatcaat atcagccaga tcggatagag caaaatcatg cgcggtgtcc ttgagcgtta
     2041 cgggcaatga gtgcgctgtg aagaaccagg cctgcggcgg caagcaacag acatacccag
     2101 gcaatcgagc gatattcggt cagaccaccg ggtgacttga gccagcttcc ccattggccg
     2161 gtgacacagt tcatcaaatt tgattcggtt tgggacaggg cattgaccgc aaaataccag
     2221 tgtggaggcg accccgaact ggtcttctgt ttggggcgga gttcaaaaac acccacctga
     2281 ttggcaggaa ggaaaacagt tttatcccgg actatgaccg attgagtttg tttttggggt
     2341 gtcacaattt caagtgaaac cgtttcagga tcaacggtca ccgccgcgtc tgtacccaac
     2401 cggcaggtgg actgatggac cccaggcgat tgagctgccc gc
//
LOCUS       DAOQDO010000865         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38344_length_2442_cov_2.855467, whole genome shotgun sequence.
ACCESSION   DAOQDO010000865 DAOQDO010000000
VERSION     DAOQDO010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38344_length_2442_cov_2.855467"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKZ53_13720"
     CDS             complement(<1..352)
                     /locus_tag="PKZ53_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41546.1"
                     /translation="MQVTLTLPNLHRYQNWVANNSSRWNILCCGRRFGKTTLGIELLL
                     DPALDGFPTAWFAPNYKLLKEAWRELKRVASPIIKDKSESEHRIELLTKGVIEFWSLD
                     NADSVARGRKYKRAM"
     gene            complement(294..551)
                     /locus_tag="PKZ53_13725"
     CDS             complement(294..551)
                     /locus_tag="PKZ53_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41547.1"
                     /translation="MADLALAIKKGRVKTREDVANALYEECLKGNLGAIIWFEKTRFG
                     INDKVSSDSELTITIKREGEKPNASDPNLTEPPPIPELGGE"
     gene            complement(551..1399)
                     /locus_tag="PKZ53_13730"
     CDS             complement(551..1399)
                     /locus_tag="PKZ53_13730"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNJ41548.1"
                     /translation="MIDVVTTIEKNQLFGSYGHLSLLVPNRKLAIEPGQFGMLRPHQA
                     FEPILRRAMAIYRVKSSPAGTHVEFIYQVFGRGTEALRRLVVGQTVDCLLPLGKPFQL
                     DSVLVGGREAILVAGGVGSAALFLLAETLAKQGVSVRLFLGGRSAIDLIGLDDFIRIG
                     CKVHVATNDGSRGVVGFVTHPLERFLLDHAQDVKAGKYVLYTCGPPLMLRRVAEIADE
                     AQLLGYASLEERMACGFGVCVGCVVGIKEANAGLKGETDFDYQPLMKSRCHWEFQSGP
                     CTGGKP"
     gene            complement(1598..>2442)
                     /locus_tag="PKZ53_13735"
     CDS             complement(1598..>2442)
                     /locus_tag="PKZ53_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ41549.1"
                     /translation="GKEIAASYQTTFFQDYHELIDQVDAVSIAVPTVDHCRITCELLR
                     AGKSVLVEKPIAVTLAEADEMITTAQQNGCVLQVGHLERFNPAVQAARKIIKNPRFFE
                     IHRMSVFTMRSLDIDVVTDLMIHDLDILTSFVNSPLSSVHAVGIPILSAKIDIANARL
                     EFANGCVANLTASRISSEKVRKIRFFQPHDYVSIDYTEQRVGVWSLRPPNLATPHPQI
                     AEHFLDVVKDEPLRAQLAEFVRCVQTKDTPEVDGRTGRRALELGLKVVAEIQAHAART
                     GLTA"
BASE COUNT          593 a          621 c          629 g          599 t
ORIGIN      
        1 ccatggcccg tttgtatttg cgtcctcgtg cgaccgagtc ggcattgtcc agcgaccaaa
       61 actcgataac tccttttgtc aggagttcaa tccggtgttc tgattcgctt ttatctttga
      121 tgatcgggct ggcgacccgc ttgagttcgc gccaggcttc cttgagcagt ttgtaattgg
      181 gagcgaacca agcggtagga aatccatcca gggcaggatc gagcagcaat tcaataccga
      241 gggtggtttt tccgaatcgg cgaccacaac acaagatgtt ccaccggctg gagttattcg
      301 ccacccagtt ctggtatcgg tggaggttcg gtaaggttag ggtcacttgc atttggtttt
      361 tctccctctc gcttgatggt aattgtcaat tcactatctg aactaacttt gtcattgatt
      421 ccaaagcgag tcttttcaaa ccaaatgatg gcaccaagat tgcccttcag acactcctca
      481 taaagagcat tggcaacatc ctctcgggtc ttgacccttc ctttttttat tgccagtgct
      541 agatctgcca tcagggtttt ccgcctgtgc agggtccgga ttgaaattcc cagtgacagc
      601 gcgatttcat caagggctgg taatcaaagt cggtttcgcc tttgagtcca gcgtttgctt
      661 ctttaatccc aaccacacag ccgacacaga cgccaaatcc gcaggccatg cgttcttcaa
      721 gtgaagcata tccgaggagt tgtgcttcat ccgcaatttc tgccacccgt cgcagcatca
      781 gtggtgggcc acaggtatag aggacatatt tcccggcttt gacatcttga gcgtgatcaa
      841 gcagaaagcg ttcgagtggg tgcgttacaa acccgacaac gccccgtgac ccatcattgg
      901 tggcgacatg aactttgcag ccaatgcgga taaagtcatc gagtccaatt aaatcaatgg
      961 ctgagcgtcc accaagaaac agcctgactg agacgccttg tttggccagt gtttcggcta
     1021 gcagaaagag ggcggctgat ccgacgccac ccgcgacgag tatcgcttca cgtcctccga
     1081 cgagcactga atccagttga aatggtttcc caaggggtaa caggcaatca acggtttgcc
     1141 cgacgaccag gcggcgcaga gcttcggtcc ctcggccaaa tacctgataa ataaattcga
     1201 catgcgttcc agccgggctt gatttgaccc gatagatcgc catggcccgc cgcaggatgg
     1261 gttcaaatgc ctggtgtggg cggagcatac caaactgccc tggctcaatt gccagttttc
     1321 gatttgggac cagcaggctc aaatgtccat agctaccaaa aagctggttt ttctcaatgg
     1381 tggtgacaac atcaatcata aaaactaagg ggtcggggtt cagaggtcgg ggttcagggt
     1441 tcagggttca gggtttaaaa gacaaaaagg acaaaaagga cgaaaaggac ataaattcga
     1501 aaacccggaa tctggaccag atgacagaat gacctggtga tttcttttca tccctcatcc
     1561 ctcatccctc atcccttaaa ggacccggaa ccctatctca ggcggtgaga cctgttcggg
     1621 cagcatgggc ctgaatttcg gcgacaactt ttaatccaag ctcgagcgcc cggcgtccgg
     1681 tccgcccatc aacttctgga gtgtctttgg tttggacaca cctgacgaac tcagccagtt
     1741 gagcacgtaa tggttcgtct ttgacaacgt ccagaaagtg ttcggcaatt tgcggatggg
     1801 gtgtggccag gttgggaggc cggaggctcc acacgcctac ccgctgctca gtatagtcaa
     1861 tcgaaacgta gtcgtgaggt tggaaaaacc tgattttacg aaccttttca gacgagatcc
     1921 gactggcggt cagattagcc acacatccat tggcaaactc cagccgggca ttggcaatat
     1981 caattttggc cgagagaatc ggaattccaa cggcatgaac tgaactgagc ggagaattga
     2041 caaacgaggt caggatatcc aggtcatgga tcatcaaatc cgtgaccaca tcaatatcca
     2101 ggctgcgcat ggtaaagaca ctcatgcggt ggatttcaaa aaatcgaggg ttcttgatga
     2161 ttttgcgagc agcctggact gccgggttaa atcgttccag atgtccaacc tggagcacac
     2221 acccgttttg ctgggcggtg gtgatcattt catcggcttc agccagtgtg acggcaattg
     2281 gtttttcaac caggactgat tttccggccc ggagcaattc acaggtaatc cggcaatgat
     2341 caacggttgg cacggcaatg ctgacggcat caacctggtc aatgagttcg tgatagtcct
     2401 gaaaaaacgt ggtttgatag ctggctgcga tttcttttcc ag
//
LOCUS       DAOQDO010000866         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38385_length_2441_cov_2.321459, whole genome shotgun sequence.
ACCESSION   DAOQDO010000866 DAOQDO010000000
VERSION     DAOQDO010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38385_length_2441_cov_2.321459"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..541)
                     /locus_tag="PKZ53_13740"
                     /pseudo
     CDS             complement(293..541)
                     /locus_tag="PKZ53_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
     gene            671..1144
                     /locus_tag="PKZ53_13745"
     CDS             671..1144
                     /locus_tag="PKZ53_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ41550.1"
                     /translation="MLIGLYPETSGTQSAWPASRFAEVAGLLVNHYNARIALVGEAKN
                     SPVIKDVAAKLRNEYQHEVVMLAGIPVAERIAGLACCTLMVTAEAGWARLSAAVDTPT
                     VLLTDTRDEHLDPDSAGQRHHFLYQPQLEAISTDQVFDVICGMLRTSRTGALFNR"
     gene            complement(1210..2043)
                     /gene="lgt"
                     /locus_tag="PKZ53_13750"
     CDS             complement(1210..2043)
                     /gene="lgt"
                     /locus_tag="PKZ53_13750"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNJ41551.1"
                     /translation="MFPELIKLGSNFTINTYGVFIAIAFLAGVLLTAKLAEEDGLDRG
                     TMYDLCLYMLIASLVGSRVLLVITEWEQYRLNLKSLFSIDFLRSGGVFYGGLIGASLA
                     SIFLMRHYKLPWWKTADACAPGIALGQFFGRLGCFAAGCCWGKCTTSWVGVHFSTRAH
                     ELTGVPIDCALHPTQLYESISTLILCFGLLFLRKRRSFDGQIILAYFFCYAAIRFSIE
                     FFRDDPRGAVSLFGTQSQGMPLELSTSQLIALLFVVLSGLSLWFLRRRSNQERGSEGA
                     K"
BASE COUNT          624 a          665 c          638 g          514 t
ORIGIN      
        1 accacggttg agacatagca ttcctcttga aagaagatta aactccgcaa gcatcctgag
       61 cggagcaagc tgtagggcgg ctttcttagc cgccagttgc ggcgcgtatg gaaacgcgcc
      121 ctacgttacg atgatcccgt ttaagtcgga agagccaaag aaaattaaac tccgcaagca
      181 tcctgagcgg agttctgcgc gcttttcgca gaacgcagtc gaaggaggcg gctcgaacgc
      241 accaccgcgc tgcgtgacgc tacccgcatc cttcgactgc actccgttcc gctcacgccc
      301 tgtccgcgaa gcgggggcgc gctaggcggg atgcttgact cgctattcgc ttcagccact
      361 taatctgctc cgccacttga tacgtatcgc cgccctcgtg atgattgtac gcccactcga
      421 tgatgcttct cggcccggcg taatgattgt aagcggcaaa caccgtgcgc ggcgggcaaa
      481 tctcatccat caaacccacg gagaataacg ccggaacagt cgcccgcgcc gcaaagttga
      541 tcaatatgcc gaagcgattg aagcgattgg ggttcaggtc attgaccgca acattcgtct
      601 tccgattgcc ggcaagtttg acacggcgat tcaaaaacgg ctgaagcgat caccctggaa
      661 tccaggggaa ttgctgattg gtctctaccc tgaaaccagc ggtactcagt cagcctggcc
      721 tgcctcgcgc tttgccgaag tggctggatt gctggtcaat cattacaacg cccgcattgc
      781 cctggttgga gaggccaaaa acagtccggt gatcaaagac gtcgctgcaa aattgcggaa
      841 cgagtatcaa catgaagttg tgatgctggc cggcatcccc gtggctgaac gaattgccgg
      901 actggcctgc tgtaccttga tggttacggc tgaagctggc tgggccaggc tttcggcagc
      961 cgttgatacg ccgacagttt tgttgactga tacgcgtgac gaacaccttg atccggattc
     1021 tgctggacaa cgccatcact ttctatatca acctcagctt gaagccatct caaccgatca
     1081 ggtttttgac gtgatttgcg ggatgctccg caccagccgg actggcgccc tgtttaaccg
     1141 atagtcattt gagcaaaata aatcaggggt tgattgggtg acagcatcaa ctcgaaacgt
     1201 aacacgttgc tacttcgctc cttcgctccc tcgttcctgg ttgctccggc ggcgcaaaaa
     1261 ccagagactc aggccagaca gcacgacaaa gagcagggca atgagttgtg acgtcgagag
     1321 ttccagtggc atgccttgcg attgggttcc aaagagagaa acagccccgc gtgggtcatc
     1381 acggaaaaac tcgatagaga atcgaatggc ggcgtaacaa aagaagtaag ccaggatgat
     1441 ttgtccatca aaagaccgcc gttttcgcaa aaacaacagt ccaaaacaga gtatcagcgt
     1501 gctgattgat tcatacaact gggttgggtg gagggcacag tcaattggaa ccccggtgag
     1561 ttcgtgggcc cgggttgaaa agtgaacccc aacccagctt gtggtgcatt ttccccagca
     1621 gcatccagcc gcaaaacagc ccagccggcc aaaaaattgc cccagggcaa tgccaggggc
     1681 acaggcatct gcggttttcc accaggggag tttatagtgg cgcatcagaa agatgctggc
     1741 gaggctggcc ccgatcaatc cgccataaaa cacaccccct gagcgaagaa aatcaatgga
     1801 gaagagcgat ttcaggttga gtcggtattg ttcccactcg gtgatgacca gcaatacccg
     1861 tgatccaacc agggaggcga tgagcatata gaggcacaaa tcatacatcg taccccggtc
     1921 aagtccgtct tcttcagcca gtttggcggt gagcaggacg ccggccagga aggcaatggc
     1981 aatgaacact ccgtaggtat taattgtaaa attggagcca agtttaatca gttcaggaaa
     2041 catggtaact actttcactt ttggtcagac ttgaggcgcg gaacccgccg ccaaagccgg
     2101 gaatcaacaa aaatctgcaa gaagaatggc aaaaaaacat caaatgcggt actttcaagc
     2161 tgcccttgca cgctaccaca agcctttttc aaatcacaaa ttcctttcct gcagtaacaa
     2221 caaagaacgc gttccggtaa tagtttcaaa aaagctcgga cactgcgacc acaaacaagg
     2281 atccgcctat gcctgtgacg agttcacccc gaccgttgat tgggatttca acccggttaa
     2341 atccaactga cgatacaaat tacttacgtc aaacctatgg ccgggcggtt ttcgccgctg
     2401 gcggagttcc cgtgctggtg ccgatcatcc ctgacgccgt g
//
LOCUS       DAOQDO010000867         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38467_length_2438_cov_3.325640, whole genome shotgun sequence.
ACCESSION   DAOQDO010000867 DAOQDO010000000
VERSION     DAOQDO010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38467_length_2438_cov_3.325640"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKZ53_13755"
     CDS             <1..382
                     /locus_tag="PKZ53_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ41552.1"
                     /translation="FNAEVIGCMPGPDGEKIMHAELRIGDSPLMLCDEFPEMGSLSPE
                     SRGGTSVSLHVYVVNADETYQQAVDAGASVIMPLADMFWGDRYGIVADPFGHHWAVAT
                     HIQDLTVEEITAAGAAAMAAGGCQ"
     gene            530..2242
                     /locus_tag="PKZ53_13760"
     CDS             530..2242
                     /locus_tag="PKZ53_13760"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNJ41553.1"
                     /translation="MSLKPKAMGSIPEMTCRIGQQILKPENVYRQMGEKYADVVRDED
                     FVKMYSHLGQPAISPARLAMVVVMQAMEGVSDRVAAEMVRTRIDWKYALHLELEDHGF
                     DASVLSEFRQRIVSQEAERKVFDAFVNRFQEEGLLKGRGLQRTDSLGVVAAVRELNRL
                     ELVMETMRKALEGVVEANQEWGRGVVSARWIETYETWTQGERVVKGSGPEAKSETLRR
                     LEAVGRDGYQLLEMIEAEGTPKAVREARGVEILKQVWSQQYRLVNAPGVTRIELSTVA
                     SRAQDGVSELIQTPHDVEARYRIKRGVGQTGYTLQITEVAGEAAPAIITDVAIEAGNA
                     DDTKAVAAIHQRLTERGLTPTTHLVDSGYLSGNILVESAQRGVELLGPLQDDSSPAAQ
                     AGLGFGLEHFDLSFETRTAICPGGQPAARWTPFEDVTRKRELIQIAWTAATCGRCEFR
                     TACVSGQTGRFLKVSHRFPTIKARRQYQKTPEFRQRYRRRAGIEATFSTMVNTCGARR
                     TPYRGRHKTLVKYAALATAINLRRAIAWKTGVHPQRHRRSRLRHLLGIPQVNQSSQPE
                     ESAR"
BASE COUNT          600 a          538 c          793 g          507 t
ORIGIN      
        1 tttcaacgcc gaagtcattg gctgtatgcc gggaccggat ggtgaaaaaa ttatgcacgc
       61 tgaattacga atcggtgatt cacccctgat gttgtgcgat gaattcccag aaatgggcag
      121 cctgtcaccc gaatcgcgag ggggcacatc ggtctcgttg catgtgtatg tggttaacgc
      181 cgatgaaacc tatcagcagg cagtggacgc tggtgcgagc gtgatcatgc cgctggcaga
      241 tatgttttgg ggagatcgct acggaattgt tgcagaccca tttggtcatc actgggctgt
      301 ggcgacccat attcaggatc tgacggttga agaaattacc gcagcgggag cggcggcaat
      361 ggcggctggt ggatgccagt agtttataga ttaccggcag aaacccccgg aatttatgtt
      421 actgctggcg aagtctaaaa agttgatttc ctgatgaagc gatggaactg gacgaggtat
      481 attgtcgagg aaggagtgaa ggaagtggga agaagggagg agcaggcaga tgagtctcaa
      541 accaaaggca atgggatcga tcccagaaat gacctgccgg atcgggcaac agatcttaaa
      601 accagagaat gtgtatcgac aaatgggaga gaagtatgcg gatgtggtga gggatgaaga
      661 ttttgtgaag atgtattcgc atcttgggca accggcgata tcaccagcgc ggttggcgat
      721 ggtggtggta atgcaagcga tggaaggtgt gagtgaccgg gtggcggcgg agatggtgcg
      781 aacgcgaatt gattggaaat atgcgttgca ccttgaatta gaagaccatg gatttgatgc
      841 gagtgtgttg agcgaatttc ggcaacgaat cgtgagtcag gaagcggagc ggaaagtgtt
      901 tgatgcgttt gtcaaccgat ttcaagagga aggattgctc aagggacggg gtttgcagcg
      961 gacggacagt ttgggagtgg tggcggcggt gcgggagttg aatcggttgg agttggtgat
     1021 ggagacgatg agaaaagccc tggaaggagt ggtggaagcg aatcaggagt gggggagagg
     1081 ggtcgtgtcg gcaaggtgga tcgagacata tgaaacctgg acgcaagggg aacgagtggt
     1141 gaaaggaagc gggccggaag ccaaaagcga gacgctcagg cggttggagg cggtggggag
     1201 ggacgggtat cagttgttgg aaatgattga ggcggaaggg acgccgaaag cggtgcggga
     1261 ggcaagagga gttgagattt tgaagcaggt atggagtcaa caatatcggc tggtgaacgc
     1321 acctggagtg actcggattg agttgagcac ggtcgccagt cgggctcagg atggagtgtc
     1381 ggagttgatc caaacgccgc acgacgtgga ggcgagatat cggatcaagc gtggggtggg
     1441 acagaccggg tacaccttgc agattacgga agtggctggt gaagctgctc cagccatcat
     1501 cacggatgtg gcgattgaag ctggaaatgc ggatgacacc aaggccgttg cggccattca
     1561 tcaacggttg actgaacgcg ggttaacccc gacgactcac ctggtggaca gtggatacct
     1621 cagtggcaac atcctggtcg aaagcgcgca acggggtgtc gaattgctcg ggccactcca
     1681 ggatgattcg agtccggcgg cacaggccgg gttggggttt ggccttgagc actttgatct
     1741 cagctttgaa acgcggacgg cgatctgtcc cggaggccaa ccggcagcca gatggactcc
     1801 cttcgaggac gtgacgcgaa aacgggaatt gattcaaatt gcctggacgg ctgccacctg
     1861 tggcaggtgt gagtttcgta ccgcctgtgt ttccggccaa accggacgct ttctcaaagt
     1921 ttcacatcgc ttcccaacca tcaaagcccg ccgccagtac caaaaaacgc ccgagtttcg
     1981 ccaacgctac cgtcgccgag ccggcattga agccactttt tcgaccatgg tcaatacctg
     2041 cggcgcacgc cgaactccgt atcgggggcg gcataaaacc ctggtcaagt atgctgcctt
     2101 agccactgcc atcaacttac ggcgagccat agcttggaaa accggtgtcc acccacaacg
     2161 acatcggcgt tcacgattgc ggcatttgct tgggattccc caagtgaacc agagttcaca
     2221 accagaggaa tctgctcgat aaaggcgggt ttttccccaa aaacccttgc tcctaaccct
     2281 tttaagcttt ctgataacga gagtttggat ttttccaaac cgcgttgacc gacttcgcca
     2341 gcagtaacat aaattgcggg gattcccttg tgtgacaact cactgccgtt actgacacaa
     2401 aggaacgggg ccaccagtga cggattcccc aggttcac
//
LOCUS       DAOQDO010000868         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38474_length_2438_cov_3.025598, whole genome shotgun sequence.
ACCESSION   DAOQDO010000868 DAOQDO010000000
VERSION     DAOQDO010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38474_length_2438_cov_3.025598"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKZ53_13765"
     CDS             <1..363
                     /locus_tag="PKZ53_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41554.1"
                     /translation="GDGGLAESAILAGPSGITVDKDGTIYFIDSRNNRIRRIDPKTKR
                     IKTIAGSGSRKFSGDGGPATLAGLGFEYPTNYNVINNSDLVADGLGNLYVADTFNNCV
                     RKINLVTGVITTAAGIPL"
     gene            complement(513..1766)
                     /locus_tag="PKZ53_13770"
     CDS             complement(513..1766)
                     /locus_tag="PKZ53_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ41555.1"
                     /translation="MTQFHPIESLADLRHEFGEHGGVNLSIEASSTFTVMKAATLPAI
                     FKGQQGPENGGCYLYGRHYNPTVSVLAKQIAALEGAEAAYCTSSGMSAIAATILQLTS
                     QGDHIVSSDTVYGGTFALLKEFLPAKMGLGVSFVNTDDLAAVDAAMTPQTKLIFVETM
                     SNPTLRVADIPRLAEIAHRHGAKLVVDNTFCPLILSPIRLGADIVVHSLTKFINGASD
                     FIAGAICGPLDFIHTLMDLHMGSLMLLGPTMDPKVAHEISLRLPHLGLRMREHCQRAQ
                     VFAGRLQSLGFSVIYPGLPSHPNYQLMQDLHNDEYGFGGLVCLDLGTSQQANRLLEYL
                     QNEKGFGFMAVSLGYFDTLMTCPATTTSSELTDDELRRAGILPGLVRMSVGYTGSLEQ
                     RWKQLEEALQALELLPAQKTQLSAA"
     gene            1888..2367
                     /locus_tag="PKZ53_13775"
     CDS             1888..2367
                     /locus_tag="PKZ53_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNJ41556.1"
                     /translation="MENPELDRIDFRILAELQNNARLSNKELAAIVDLAPSTCLERVR
                     RLRSIGVLRGAHEQVAPAALGIGLQALIAVRLKRHSREIVESFQAHILGLREVVAVYH
                     LAGATDFLIHVAVKDDKHLRNLLLDSFTTREEVNQTETSLIFDHRWKPKMPNFVEEG"
BASE COUNT          639 a          612 c          652 g          535 t
ORIGIN      
        1 ggtgatggcg gattggccga aagtgcgatt ttggccgggc catctggcat caccgtcgat
       61 aaagacggca ccatttattt cattgactcg cgcaataacc gcattcgccg gattgaccca
      121 aaaaccaaac gtatcaaaac gattgcggga agcggatcac gaaagttttc tggcgatggc
      181 ggaccggcaa ctctggcggg gctgggtttt gaatacccga caaactataa cgtgatcaac
      241 aattcggacc ttgtggctga tgggcttggg aatctctacg tggcggatac ctttaataac
      301 tgtgtccgta agatcaacct ggtcaccggc gtgatcacga cagcagcggg aattccactc
      361 taatggcgtg acaccgccgg acctcaaagc ggcgtcaagc cgccgcactc cacaataaaa
      421 acgaaagcgg cgtcaagccg ccgcactccg aaaaaaattc ccgttccgga aattttccgg
      481 aacgggaaaa tttggactct caaacggttg tctcacgcag ccgagagttg ggttttctga
      541 gcgggcaaca actccagagc ctgaagagcc tcttcgagtt gcttccagcg ttgttccaga
      601 ctgccggtat aaccgaccga catccgcacc agtccgggga gaatcccagc ccggcgaagt
      661 tcgtcgtccg tcaattcgct tgacgtcgtg gtggccgggc aggtcatcag cgtgtcgaaa
      721 taccccaaac tcacggccat aaatccaaag cccttttcat tttgcaggta ctcaagcaac
      781 cggttggcct gctgcgaggt gcctaaatca agacacacca gcccgccaaa tccatattcg
      841 tcattgtgaa gatcctgcat caattgataa ttcggatgtg atggcagccc tgggtaaatc
      901 actgaaaatc caagtgattg caaccgcccg gcaaaaacct gggctcgttg acagtgttcg
      961 cgcatccgca acccaaggtg cggtaaccgg agactgattt cgtgggcaac ttttggatcc
     1021 atcgtcggcc caagcagcat caatgatccc atgtgcaggt ccatcagcgt gtggataaag
     1081 tccagcggcc cgcaaatcgc tccggcaata aaatctgacg caccgttgat aaacttggtc
     1141 agactgtgaa ccacaatgtc ggctccgagc cggattgggg acagaataag tggacaaaag
     1201 gtgttgtcca ccaccagttt ggcaccgtgg cgatgggcaa tttctgccag acgtggaatg
     1261 tcagccactc gaagtgttgg gtttgacatc gtctcgacaa aaatcaactt tgtctgcgga
     1321 gtcatggcgg cgtcaaccgc agccaggtca tcagtattta caaatgaaac gccaagtccc
     1381 attttggcgg ggaggaattc tttcaagagg gcaaacgtgc caccgtacac ggtatcgctc
     1441 gatacaatgt ggtcaccttg cgaagtcagt tgcaagatgg tggcggcaat cgcgctcatc
     1501 ccgctggagg tacaataggc ggcttcagca ccttcgagcg ccgcaatttg tttagccagc
     1561 acgctaacgg tcggattgta atgccgaccg tacaaataac agccgccgtt ttcgggtcct
     1621 tgctgtccct taaagatggc tggcagcgtg gcggccttca tcacggtaaa ggttgagctg
     1681 gcttcgattg aaaggttgac accaccgtgt tctccgaatt cgtgacggag atcggccaaa
     1741 gattcgatgg gatgaaactg ggtcatcacg tgaatcctcc aaattgtaag atgagtgaag
     1801 ggaagcgaaa gaaattctga ggcaactgat gaaaattact atatattcag aaaaacactt
     1861 tgtatatcga attgaattca gaaaaatatg gaaaatccag agctagaccg aattgacttc
     1921 agaattttgg ccgaattgca gaacaatgca cggttatcga acaaagaact cgcggccatc
     1981 gtggatttag cgccttcaac ctgcctggaa cgggtcaggc ggttgcggtc aatcggcgtg
     2041 ctgcgcgggg ctcacgaaca ggtggctcca gccgccttgg gcattggatt acaggcattg
     2101 attgctgtcc ggctcaagcg ccattcccgg gaaatcgtgg agagttttca ggcacatatc
     2161 cttgggctac gtgaggttgt ggcggtatat catctggctg gcgccaccga ttttctgatt
     2221 catgtggcgg tcaaagatga caaacacctg cgcaacctgc tgcttgattc attcacgact
     2281 cgcgaagaag tcaatcagac ggaaacatca ctgatttttg atcatcggtg gaaacccaaa
     2341 atgccgaatt ttgttgagga ggggtgaagg cttcgggttc cggggtcaga aaaagacaaa
     2401 aaggaccaaa aggacaaaaa ggaccgaaag gacataaa
//
LOCUS       DAOQDO010000869         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38519_length_2436_cov_3.065099, whole genome shotgun sequence.
ACCESSION   DAOQDO010000869 DAOQDO010000000
VERSION     DAOQDO010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38519_length_2436_cov_3.065099"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..>2436
                     /locus_tag="PKZ53_13780"
     CDS             333..>2436
                     /locus_tag="PKZ53_13780"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNJ41557.1"
                     /translation="MPSLNGKDAIQFLKQEGVYDSLNDALTKASFGANAIPTTQQAQL
                     VAPDGAASDTFGCSIAISGETVVVGSRFGDGGGVFNQGTVHVFVRTGTVWSLQQKLLP
                     VDGSPGQLFGGSVAIDGDTVVIGSPQATISGNMFQGAVYVFARTGSVWSQQQKLTAFD
                     GAAQDQFGQVVSISCDTLVVSARLADVGGNTDQGAAYVFVRTGVTWSLQQKITAADGQ
                     VSDNFGASVAIHHETLAIGAPGDDIGPALNRGSVTILVRTGTTWNVQQVVTTPDGISG
                     INFGIAVAIQSDTLAVGANGFFDSNTGSVTGTVFLFTRSSTNWSLQQRVGPSVATSSG
                     QFGISVVLSGETLVVGSPGATVGVNTNQGAAYVFVRTGATWTEQQRIVTSDGMSNNQF
                     GQSVGISGETVVAGALGKMIGSNPFQGAAYVFACTAINRTWTQQTGLVSIDGSLGDQL
                     GFSVAISGETAVVGVPPDDFGSNFDQGSAYVFLRIGTTWVQQQKLTAADGAMNDQFGF
                     SVAISGETIVVGAHLDDTGATSDHGSAYVFVRTGGTWSQQQKLTASSPASGDRFGAAV
                     SISDDTLVVGAVQDDVGANPDQGSASVFTRTGSVWSLQQQLMAPGGAASDLFGTSVAI
                     EYNTLVVGAPFDDAIGVNDGSVVVFVRSGTTWSQQQQLTAPDAADGDGFGSAVALNRE
                     TIVVGSPGDDSPANADQGS"
BASE COUNT          508 a          598 c          732 g          598 t
ORIGIN      
        1 cctcatccct tcgattgccc caagccccaa gcccggtttc ttcagcccga agtctccagc
       61 cccaagccct aagcccgaag tcttcagctc gaagtcttca gccctgagtc ctgatttatt
      121 cagccgcatt ccttatgcct tcacaggtat tacatttaca gaaagcgggt tgaaatatcc
      181 gtatgcgcag aatacacaat attgtctctc ctggaatttt attgactgtt cttgctttga
      241 gtttccttgg aggagttagt ctcatttatc ctcactcagc aactgctcaa gcagaactgg
      301 ttctcgcaaa atcttcacgt gatcaatctg gcatgccatc actcaatggc aaagacgcga
      361 ttcagtttct caagcaggaa ggcgtctacg attcattgaa tgacgcttta acgaaggcga
      421 gctttggcgc caatgcaatt ccaaccacac aacaggctca actggtggcg ccggacggtg
      481 cggcctccga tacctttggc tgttcaattg caatcagcgg cgagacagtt gtggttggaa
      541 gtcgctttgg agatggtggc ggtgttttca atcaaggcac tgtgcatgtc tttgtgcgga
      601 ccggtaccgt gtggagtctg cagcaaaaat tgcttcccgt ggatggcagc ccagggcagc
      661 tatttggcgg gtcggtggcg attgatggcg atacggtggt gattggctcg cctcaagcaa
      721 ccatcagcgg gaatatgttc cagggggctg tgtatgtgtt tgcccggaca ggttcggtct
      781 ggagtcaaca acaaaaattg acggcttttg atggagcggc acaggaccag tttggtcaag
      841 tggtttccat cagttgtgac accctggttg tgagcgcgcg gttggccgat gtcggtggca
      901 acactgatca aggggccgcc tatgtttttg tccggacggg ggttacctgg tcgctccaac
      961 agaaaataac agcggcagat ggccaggtca gcgacaattt tggtgcttcg gtggcgatcc
     1021 atcacgaaac actggcgatt ggggcgccag gagatgacat cggaccagct ttgaatcggg
     1081 gttcagtcac gattttggtg cgaactggga ccacctggaa cgtgcagcag gtcgtgacca
     1141 caccggatgg gattagcgga atcaattttg gtatcgctgt ggccatccag agcgacaccc
     1201 tggcggttgg ggccaatggt tttttcgatt ccaacacggg ttcagtcact ggcaccgtct
     1261 ttctctttac tcgaagcagt actaactgga gtttgcaaca gcgggtggga ccttcggttg
     1321 cgacttcaag cgggcagttt ggaatttcag tggttctcag cggtgagacg ctggtggtcg
     1381 ggtcacctgg ggccaccgtt ggtgtgaata ccaatcaagg ggcggcctat gtgtttgtca
     1441 ggaccggcgc cacgtggacc gagcaacaac ggatcgttac ttcagacggc atgagcaata
     1501 accagtttgg acagtcggtt ggaatcagtg gcgaaacagt ggttgccggc gccttgggga
     1561 aaatgattgg aagcaatccc tttcagggcg ccgcctatgt gtttgcctgc acggcgatca
     1621 accgaacctg gactcaacag accggtctgg tttcgattga cggaagcctt ggtgaccagc
     1681 ttggattttc ggtcgccatc agtggggaaa ccgcagtcgt cggagttcct ccggatgact
     1741 ttggcagcaa tttcgatcag ggctcagcct atgtgtttct ccgaattgga accacctggg
     1801 ttcagcaaca gaaattgacg gcggcagatg gggcgatgaa tgaccagttt gggttttcag
     1861 tggccatcag tggggagaca atcgtggttg gtgcccatct ggatgacact ggtgccacct
     1921 ctgaccatgg ttcggcctat gtgtttgtcc gaaccggtgg cacctggagc cagcagcaga
     1981 agttaacggc ttcaagcccg gcttccggtg acaggtttgg ggcggcggtc agcatcagtg
     2041 acgatacgct cgtggttggg gctgtccagg atgatgtcgg tgccaatcca gaccagggtt
     2101 cagcttcggt gtttacccgg actggttcgg tgtggagcct gcaacaacaa ttgatggcac
     2161 ctggtggagc ggcttctgat ctctttggca ccagtgtcgc catcgaatac aacacccttg
     2221 tggttggggc gccgtttgat gatgccatcg gggtcaacga tgggtccgtt gtggtgtttg
     2281 tccgttccgg aacaacctgg agtcaacaac aacagttgac ggcgccagat gctgccgacg
     2341 gtgatggatt tggaagtgcc gttgccctga accgggaaac gattgtggtt gggtcgccgg
     2401 gtgatgacag tccagcgaat gctgatcaag gttcag
//
LOCUS       DAOQDO010000870         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38594_length_2433_cov_3.610597, whole genome shotgun sequence.
ACCESSION   DAOQDO010000870 DAOQDO010000000
VERSION     DAOQDO010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38594_length_2433_cov_3.610597"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PKZ53_13785"
     CDS             complement(<1..141)
                     /locus_tag="PKZ53_13785"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNJ41558.1"
                     /translation="MITVFGGARCLPETLEYKQALQLGRSLAEAGYTVCTGGYAGVME
                     AIS"
     gene            complement(291..656)
                     /gene="pheA"
                     /locus_tag="PKZ53_13790"
     CDS             complement(291..656)
                     /gene="pheA"
                     /locus_tag="PKZ53_13790"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNJ41559.1"
                     /translation="MSVKPAGPERMFFLADQSLLWPEFMDIQHWREQIDAIDDELVKL
                     INQRAVFVAEIARLKQMAQLPVHIPEREAQIHARIRTTNPGPLRADALQRIFECIICE
                     SRQLEHQILEEADSSASHR"
     gene            1126..2316
                     /locus_tag="PKZ53_13795"
     CDS             1126..2316
                     /locus_tag="PKZ53_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ41560.1"
                     /translation="MRTFSFRQVLLIVLLAVAANTVVSNALIEYRTRPTLAASAVTTP
                     VEITSPTVASDEQNNVEIYRLASPGVVHITSTVDVQSFFGAYPQQGTGSGSIIDDKGH
                     ILTNYHVVRDANNLDVTLSDKSTYKARLVGADPDNDIAVIQIDAPANKLNVMQLGASG
                     NLEVGQKVLAIGNPFGLDRTLTTGVISGLNRPLQTEDGRTMENVIQTDASINPGNSGG
                     PLLNKFGQIIGINTAIYSPTGSSVGIGFAVPIDTAKQIIPELIASGKIRRGYLGVTTA
                     PLTPKIAEQFNVPTKQGVVVVSVQPNSPASQAGLLGVQQDQEQLGDVIVKADGKPVET
                     PDALVKTIGNRKPGDRIALTVLRDGRERQVDVTLADRPLQISQNRPQQRVQPRGNLFD
                     WFRK"
     gene            complement(2253..>2433)
                     /locus_tag="PKZ53_13800"
     CDS             complement(2253..>2433)
                     /locus_tag="PKZ53_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41561.1"
                     /translation="QAPGTFFILHSSFFILHSQFFILHSQFFILHSPYLHSQTYLRNQ
                     SNRLPRGWTRCCGRF"
BASE COUNT          645 a          576 c          627 g          585 t
ORIGIN      
        1 gctaatagct tccatcactc cagcataccc gccagtgcag acggtatatc cagcttcagc
       61 cagactccgg cctaactgga gcgcctgttt gtattcgagt gtttccggta aacagcgggc
      121 accgccaaaa acggtgatca tcgggggcgt ttttcgggaa tgtggatggc tcgtcatatc
      181 cgttcacctg ttgagaccag cagaagtaca ttgaagttat aggttatcaa gtgtctgtta
      241 ttcaatgaga tagcgtttct gtagcttgtt agatatgact cgtacctgtg ttaccgatga
      301 gaggcacttg agtcggcttc ttccaggatt tgatgctcaa gttgacggga ctcacaaata
      361 atacattcaa aaatgcgttg caaggcgtca gctctgagcg gccctgggtt ggttgtccga
      421 atccgggcat gaatttgtgc ttctcgctcc ggaatatgaa ccgggagttg tgccatctgt
      481 ttcaaccggg caatttcggc gacaaacacc gcacgctggt tgatcagctt tactaattca
      541 tcatcaattg catcaatttg ttctcgccaa tgttgaatat ccataaattc gggccacaaa
      601 agagactggt cagccagaaa gaacatccgt tctggacctg caggcttgac tgacaaagta
      661 aagaagatga ttgagaggga tgtggtcgcg ttttcaaacc atacgcaccc tggtttcaga
      721 aggcaagtcc gctgaaaaag tgacaggctg atcaataaca gaatgacatg tgaccagata
      781 cctgctggtc attctgtcat cctctgtggc atctcgcccc acccggtaca agtttgcctc
      841 agcctcaagg gtggataatc tgggcgcaag tctggctggg ttgcttaatt ctctaaatcg
      901 aagtggttat cgccgttagc cagtttttta agttattggc acagtagttg ctctgaccaa
      961 tgatgaaaga aaagttgatt tttggaaata ctgaagaaac ggccactggg ccgaaacctt
     1021 gagcctggtt tggtggtgta cctccgcacc aaacacaact caggtgagta cctgggccgt
     1081 caataaaaca aaatctttta acatcgtcga ggagatccaa attgtatgcg aacattttca
     1141 ttccgacagg ttttattgat tgtgttgctc gccgtggctg ccaatacagt tgtttcaaat
     1201 gcgctgatcg aatatcgaac ccggccaacg ctggcagcgt cagccgtgac caccccggtt
     1261 gaaatcacca gcccaacggt tgcctcagat gagcaaaaca acgttgaaat ctaccggctg
     1321 gccagtccag gcgtggttca cattaccagc acggtggatg tgcagagctt ttttggcgcc
     1381 tatccccaac aaggtaccgg gtcagggtca attattgatg ataaaggaca tatcctgacc
     1441 aactatcacg ttgtgcgcga tgccaacaac ctcgatgtta cgttgtctga caagagcacc
     1501 tacaaggcac ggctagttgg ggctgatcca gataatgaca ttgcggttat ccaaattgat
     1561 gctccggcca ataaattaaa tgtcatgcaa ctgggggcat ccggcaatct cgaagtcggt
     1621 caaaaggtgc tggcgattgg aaatccattt ggattagatc gaaccctgac cacgggtgtc
     1681 atcagcgggc tcaatcgccc gctccaaact gaagatggtc gcacgatgga aaacgtgatt
     1741 caaacagatg cctcgattaa ccctggcaat tccggcgggc cgctcctgaa caaatttggc
     1801 cagattattg ggatcaacac cgcgatctac agtccaaccg gctccagtgt tgggattggg
     1861 tttgccgtgc cgattgatac tgcgaaacag atcattccgg aactgattgc atcagggaaa
     1921 attcgacgtg gctacctcgg tgtgaccacg gcgccgctga ctccaaaaat tgccgaacag
     1981 tttaatgtcc caaccaaaca gggagtcgtt gtggtcagcg ttcaaccaaa cagcccggcc
     2041 agccaggcgg ggttgctggg agtccagcag gatcaggagc aactgggcga cgtgattgtc
     2101 aaagcggatg ggaaacccgt tgaaacccct gacgcgctgg tcaaaaccat tggtaatcgg
     2161 aaaccagggg accgaattgc gctgactgtg ctgcgtgatg gtcgtgaacg tcaagttgac
     2221 gttacacttg ccgaccgtcc acttcagatt tctcaaaatc gcccacaaca acgcgtccag
     2281 ccccgtggca acctgtttga ctggttccgc aagtaggtct gtgaatgaag atatggagaa
     2341 tgaagaatga agaattgaga atgaagaatg aagaattgag aatgaagaat gaagaatgaa
     2401 gaatgaagaa tgaagaaagt acctggtgct tgg
//
LOCUS       DAOQDO010000871         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38600_length_2433_cov_2.872161, whole genome shotgun sequence.
ACCESSION   DAOQDO010000871 DAOQDO010000000
VERSION     DAOQDO010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38600_length_2433_cov_2.872161"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKZ53_13805"
     CDS             <1..281
                     /locus_tag="PKZ53_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41562.1"
                     /translation="IPIITVGERSSDGSKRKLLPWVNAITPDASIVQIYWQMVLKRWN
                     PGFDLFRLHQFAHRAWIQKYHRPPPGLNIPVPEKNRQVPCDIIESPGH"
     gene            381..833
                     /locus_tag="PKZ53_13810"
     CDS             381..833
                     /locus_tag="PKZ53_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41563.1"
                     /translation="MEREELRQWLEVCWGTELFEKFLFAYNCENRRLNCLSYWQEQLL
                     QQFEAETSFHLDQFDLTHDEIFGICPVHRCQLLADEVPIRYGLLNRTPKMVELNRQFP
                     FANTHVSGGCEIQDEYQAAVVYCALCRDGLLKAQPPIHLKKSHPARKR"
     gene            892..1545
                     /locus_tag="PKZ53_13815"
     CDS             892..1545
                     /locus_tag="PKZ53_13815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ41564.1"
                     /translation="MNHLVLLGDSILDNSAWAYNGPEMCVHLRELIADDWQVTLLAVD
                     GHTTQDVVRQLELVPPTASFLVISAGGNNALRYLPVLMESVTTTAEAMEKLYQVIEEF
                     EVDYLEMLTAAQSFKCPVAVCTVYNPRFPDPKWQQAVLATLALFNDCIVRCAVRMNIP
                     VIDLRMVCTEETDFTNAIEPSAQGGRKIAEAIATLLGNVRVHTNKSEIFGVKWKTSG"
     gene            complement(1649..>2433)
                     /locus_tag="PKZ53_13820"
     CDS             complement(1649..>2433)
                     /locus_tag="PKZ53_13820"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41565.1"
                     /translation="GLLAHRTKGRWRNTQENVFVLLALDRYFTTFEKEAPDFIARTWI
                     GSAFAGETQYTGRTTDQHRLEVPMNWIAANQGPQTVTLSKEGTGRLYYRIAMNYVPVA
                     LDFKPYDAGFNVTRAYEAVDDPSDVVQVSASEWHIRAGARVRVVVTMQLPFRRYHVAL
                     VSPLAAGMEMLNPVFKTTQHPSSLEITRRFVHQWSEFQSLRNDRAEAFSTLLGQGTYQ
                     YTYLTLATTPGRFIVPPAKAEEMYAPETFGRGQTDVVVIKAE"
BASE COUNT          608 a          624 c          604 g          597 t
ORIGIN      
        1 ttattccaat cattacagtg ggagaacgtt cgtcagacgg ttccaaaaga aaattacttc
       61 cctgggttaa tgcaatcaca ccggatgcct ccatcgttca aatttattgg cagatggtgc
      121 tgaaaagatg gaacccgggg tttgatctat tccgcctcca tcaattcgca cacagagcct
      181 ggattcaaaa gtaccacagg ccacctccag gattgaatat tccggtgcct gaaaaaaatc
      241 gacaggtacc ttgtgatatc attgaaagcc cagggcactg aggtcaggac tctgcaaaat
      301 acaattttca ataatgcagc tcgattccaa aatgatatga gacccacaca ccttactgcc
      361 cagaaaggaa accagttcac atggaacgtg aagaactccg ccaatggctc gaagtctgct
      421 ggggaaccga actctttgag aaattcctgt ttgcctacaa ttgtgaaaat cgccgattga
      481 actgcctctc ttactggcaa gagcaattgt tacaacagtt tgaagctgaa accagttttc
      541 accttgatca gtttgatctg acccacgatg agatttttgg aatttgcccg gttcatcggt
      601 gtcaactgct ggccgatgaa gttcctatcc ggtatggact gttgaaccgc actccaaaga
      661 tggttgagtt gaaccggcaa tttccctttg ccaatacgca tgtttcggga ggttgtgaaa
      721 tccaggatga atatcaagcc gcggttgtgt attgtgctct gtgtcgagac gggcttctga
      781 aggctcaacc gcccattcat ctgaaaaaat cgcaccctgc caggaaacgg taacgccgtc
      841 ctcctgaata aaaaacctgt tttcctcaac tctgaacttg aaaagaatcc catgaatcat
      901 ctggttttgc ttggcgactc aattttagac aacagtgcct gggcttataa cgggcctgaa
      961 atgtgcgtac atttacgcga actgattgcc gatgactggc aggttaccct tctggcagtt
     1021 gatggacaca ccacccagga tgtcgtcagg caactggaac tggtgccgcc tacagccagt
     1081 tttctggtca taagtgctgg cggaaataat gcgctgaggt acttacccgt cctgatggaa
     1141 tcggtcacaa ccacggctga agcaatggaa aaactgtatc aagtcattga agaatttgaa
     1201 gttgactacc ttgagatgct cacagccgcc caatcgttca agtgcccggt ggcggtctgc
     1261 accgtctata acccacggtt tcctgaccca aagtggcaac aggcggtgct ggccacactg
     1321 gcgttgttta atgattgtat tgtccggtgc gccgttcgaa tgaacattcc ggtgattgat
     1381 ttgcggatgg tgtgtaccga agaaaccgat tttaccaacg cgattgaacc ttcggcccag
     1441 ggcggacgca aaatcgccga agcgattgcc accctgcttg ggaatgtcag agttcacacc
     1501 aataaatccg aaatcttcgg ggttaaatgg aaaacgagtg gctaattcca gtttgtcatg
     1561 aggccattgt attacagata aggctaagct gttgaaagaa tcgtatttcc ctcagccctt
     1621 agcgcgatac cctcaacccc aattggtatt attcagcttt gatcacaacc acatccgttt
     1681 gcccacggcc aaaggtttcc ggagcataca tttcttcggc tttggctggc gggacgataa
     1741 accggcccgg ggtggttgcc agagtcaggt atgtatactg ataggtccct tgcccaagca
     1801 gcgtggaaaa ggcttcggcg cgatcatttc tgaggctctg aaactcagac cattgatgaa
     1861 caaacctcct ggtaatttcc agagacgagg gatgctgcgt cgttttaaaa accgggttga
     1921 gcatttccat tcccgcggcg agcggggata ccagcgcgac atgatagcgc cggaacggca
     1981 actgcatcgt gaccacgacc cggaccctgg cgcctgcccg gatgtgccat tcagaggccg
     2041 aaacctgcac tacatccgat ggatcatcaa ccgcttcgta cgctcgggtc acgttaaacc
     2101 cggcgtcata tggtttaaag tccagagcca ctggcacgta attcatcgca atccggtagt
     2161 acaaccgacc cgttccctct ttgctcaggg tgacagtttg aggtccctga ttggctgcaa
     2221 tccagttcat gggcacttcg agtcggtgct ggtcggtcgt ccggcctgtg tactgggttt
     2281 caccggcaaa ggctgaaccg atccaggtcc gggcgataaa atccggtgcc tctttttcga
     2341 acgtggtgaa ataccgatcc agcgccagca gcacaaagac gttttcctgg gtgtttctcc
     2401 agcggccttt ggttcggtgg gccagcaagc ccc
//
LOCUS       DAOQDO010000872         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38626_length_2432_cov_3.734119, whole genome shotgun sequence.
ACCESSION   DAOQDO010000872 DAOQDO010000000
VERSION     DAOQDO010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38626_length_2432_cov_3.734119"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PKZ53_13825"
     CDS             complement(<1..1371)
                     /locus_tag="PKZ53_13825"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ41566.1"
                     /translation="MQYPRVSSVVPHQLASRIQRWAQAGGLPLLCLFMIGFVASHSIW
                     TVSADQPPLSSAVITSVSAKNGKLQVKGQNFATGDKIEVDGTVLDDTKAKKNKPSTVL
                     TAVSGGNLAAGTYVMTVRSVSGSRSAPFLFDNKVSNGSPQVTIKVEPPALQVVKNEQI
                     QLTAKALDATGSELAGVAYVWSVSNPAIASVDENGKVTGLATGQVTVSAVALRTTGLS
                     TIDVTEIQTGTAGVQILTSDLVVNEGQTLSLTAIVRDEAGQPIPGAVVTWESDSPEIA
                     TVDTTGRIQGIERGFATITARSENAPRNTSASVTLSVVRVESNSSGTPSSTGSVTLDS
                     SNRVYQTDLVRQVVRRGPFGQALELYAGLTDKPGKQDGARTAALFHGPLGITIDNRQG
                     NVYVADSINNAIRCIGQNDQVTTFTGKDQPGSTDGTLAEAQFRNPRGVLVDEAGNIFI
                     ADTDNHT"
     gene            complement(1560..>2432)
                     /locus_tag="PKZ53_13830"
     CDS             complement(1560..>2432)
                     /locus_tag="PKZ53_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ41567.1"
                     /translation="LTYLISGTLFMQRIFLILGCILAFSTSLVPASVASDEPQSPQAI
                     EIGKTYALPSKILGQTRRINVYLPPGYDKSSQRYPVVYLLDGGVAEDFLHIVGIASLA
                     ADFRNLREFIVVGIEGIDRYHDLTHPTTVETERKKLPTNGGSAAFRSFLADELQPFVK
                     KTFRVTDETVLMGESVAGLFVVETLLRQPDLFEGYIAVSPSLWWDGQSLAKDASALLK
                     KRPFPANRRLYLTIADEGGEMRQAVDLVAQAVKTQAPKDFNLTFAPKENETHGTIFHP
                     AALEALRKFFATTK"
BASE COUNT          570 a          615 c          665 g          582 t
ORIGIN      
        1 ggtgtggttg tcggtgtcgg caatgaagat attgccggct tcatccacca ggacgccgcg
       61 cgggttccga aattgagcct ccgccagcgt gccgtcagta cttcctggct gatcttttcc
      121 ggtgaaggtt gtgacctgat cgttttggcc aatacagcga atggcattat tgatcgaatc
      181 cgccacatag acatttccct ggcgattatc aatggtgatg cccagcggac catgaaacag
      241 tgccgcggtt cgggctccat cctgttttcc aggtttgtct gtgagccccg catagagttc
      301 aagggcctgg ccaaaggggc cgcgtcggac gacctgccgg accagatccg tctgatatac
      361 ccggtttgag ctatcgagtg tgaccgaacc ggtacttgat ggtgtaccgg aggaattact
      421 ttcaacccgt accacagaaa gtgtgactga ggctgaggta ttgcgtggtg cattctcaga
      481 acgggcggta atggttgcaa agccgcgctc gatgccttga attctccctg ttgtgtcaac
      541 cgtggcaatt tccgggctat ctgattccca cgtgacaacc gcacctggaa tcggctggcc
      601 agcttcatcc cgaacgatgg ctgtcaatga cagtgtctga ccttcgttga ccaccagatc
      661 cgaggtgagg atttgaaccc ctgctgtgcc ggtctgaatc tcggtcacgt caatggttga
      721 aagcccggtt gtgcgcaggg ccaccgcact caccgtgacc tgtccggtgg ccagtccggt
      781 aactttgccg ttttcgtcaa ccgaggcaat cgccgggttg cttacactcc agacataggc
      841 cacaccagcc agttcgcttc cagttgcatc aagggctttg gccgtcaact ggatttgctc
      901 gttcttgact acctgaagag caggcggctc aaccttgatc gtgacctgtg gcgacccgtt
      961 tgaaaccttg ttgtcaaaga ggaagggggc agagcgggaa ccactcactg agcgcacggt
     1021 cataacatag gtgccggcag ccagattccc accgctgacc gccgtcagaa cagtggaggg
     1081 cttatttttc ttggctttgg tgtcatccag gaccgttcca tcaacttcaa ttttgtcacc
     1141 agtggcaaaa ttttgtcctt tgacctgaag ttttccattt ttggctgaca ccgaagtgat
     1201 taccgcgctc gaaagcggcg gctggtcagc cgaaacagtc cagattgaat gacttgcaac
     1261 gaatccaatc atgaacaggc agagcagggg aagacccccg gcctgggccc atcgttgaat
     1321 gcgtgatgca agttggtgcg gcacaactga ggaaacacgt gggtactgca taggcaaacc
     1381 aaaatcggat gaattcaaat gaagacaagt caacgccaaa tacagaaagg ggttttctgc
     1441 attgtggcat ctgtgcgtgg atggtgggtg agtgtgtttc ttatttcaga gcaaactaag
     1501 agagaaattc aagtcaaata gtctgaaaac ttcaacttgt gaacctggat ctgctggttt
     1561 tatttggtcg tcgcaaaaaa tttgcgaagg gcttcgagtg cggctgggtg aaaaatcgtg
     1621 ccgtgagttt cattttcctt tggtgcaaag gttaaattga aatcctttgg tgcctgagtt
     1681 ttgacagcct gagccaccag atccacggcc tgacgcattt cgccgccttc atccgcaatt
     1741 gtgagataga gccgccgatt ggccgggaac ggacgtttct tcaacaaggc tgaagcgtct
     1801 ttcgccaacg actggccatc ccaccacagc gaaggactga ccgcaatata gccttcaaag
     1861 agatccggct ggcgtagcaa cgtttcgacc acaaagagcc cggcgaccga ttcacccatc
     1921 agcaccgttt catctgtgac tcgaaaagtt tttttgacaa acggctgaag ctcatccgcc
     1981 agaaaagagc ggaaagcggc tgatccacca ttggtgggga gtttcttgcg ttcagtttca
     2041 accgtggtcg gatgggtcag gtcgtgatag cggtcaatcc cttcgatgcc aaccacaata
     2101 aattcacgca gattgcgaaa atcggcagcc agagaggcaa tgccgacaat atgtagaaaa
     2161 tcttcggcca caccaccatc aagcagatac accaccgggt aacgctggct gcttttgtca
     2221 taccctggcg gtaaatagac attgatccgt cgggtctggc ccaggatttt tgacggcaag
     2281 gcatacgttt tgccgatttc aatggcctgt ggtgactggg gttcatcact ggcaactgat
     2341 gccggtacga gacttgtgga gaaggccagt atgcagccaa ggatgaggaa gattcgttgc
     2401 ataaagagtg ttcctgaaat gagataggtt ag
//
LOCUS       DAOQDO010000873         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38647_length_2432_cov_2.506100, whole genome shotgun sequence.
ACCESSION   DAOQDO010000873 DAOQDO010000000
VERSION     DAOQDO010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38647_length_2432_cov_2.506100"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKZ53_13835"
     CDS             <1..824
                     /locus_tag="PKZ53_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41568.1"
                     /translation="KLRLVTQQFAGGELTARISPAIGRRRDEIGQLAHDFDGMAERIE
                     GLVTAQRRLLSDISHELRSPLARLYVALGLARRRSGPDAKAALDRIEHETERLNELIG
                     QLLTLTKLESHSPLADAETFDLGRLVQEVAADVDFEAQAINRGVSVTAAHSCRLTGSY
                     ELVRRAIENVLRNAISYTPEQSEVKVSLETFSSGGNQLARITVRDYGPGVPEAELNRI
                     FQPFTRVGDDRDRKTGGIGLGLAITERAVNAHGGTVQAANLPDGFEVALTLPLSA"
     gene            complement(837..2099)
                     /gene="kynU"
                     /locus_tag="PKZ53_13840"
     CDS             complement(837..2099)
                     /gene="kynU"
                     /locus_tag="PKZ53_13840"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNJ41569.1"
                     /translation="MFLPTEAFALQLDQADPLARFRNQFHQPEPSVTGEPLIYFCGNS
                     LGLQPKGVRESVLQEIDDWAQLAVHGHFKEHNPWVSYEQALLGPMAELVGAQPEEVAI
                     MNGLTVNLHLLMVSFFRPTATRHQILLEANAFPSDQYALQSQIRFHGFDPESAFLEAH
                     PRPGEDTLHTEDIVALIEEHGHKIALVMFAGVNYLTGQVFDIAAITEAAHKQGCVVGI
                     DLAHAVGNVPLRLHDWDVDFAVWCSYKYLNSGPGAVAGCFVHERHRNAPELPRFTGWW
                     GTKPQIRFEMRPTFDLQPGAAGWQLSNLPILSMAALKASLDIFHEAGILALREKSEKL
                     TSYLLSLLDQNQSGKIAVITPRNLKDRGCQISIRVFDGARTVFQRLEEAGVIGDFREP
                     NVIRVAPVPLYNTFHEVWRFAQIWQALI"
BASE COUNT          598 a          627 c          671 g          536 t
ORIGIN      
        1 agaaattacg actggtgacc caacagtttg ccggaggcga attaaccgcc cggattagtc
       61 cggcaattgg acgcagacgg gatgaaattg gccaactcgc ccacgatttt gatgggatgg
      121 ctgaacggat tgaaggactg gtcaccgctc aacgacggtt gctgagcgat atctcacacg
      181 aactgcgctc tccgctggct cgtctctatg tggcgctcgg tttggcccgt cgtcgatcag
      241 gaccggatgc aaaagccgca ttggaccgaa tcgaacacga aactgagcga ctcaacgagt
      301 taattggaca gcttctgaca ctcaccaagc tggaaagtca ttcgccactg gccgatgctg
      361 aaaccttcga tttagggcga ctggtccagg aggtggccgc agatgtggat tttgaagcgc
      421 aggccatcaa tcgaggagtt tctgtcacgg ctgctcattc gtgccggttg accggttcgt
      481 acgaactggt tcggcgggca atcgaaaatg tgctccgcaa cgcgatttcc tacacaccag
      541 aacaatctga agtcaaagtt tcactggaaa ctttttcgag tggtggaaat caactggcac
      601 gaattacggt gcgtgattac ggtcccggcg tgccagaagc agaactcaac cggatttttc
      661 agccctttac ccgggttggc gatgaccgtg accgcaaaac tggcggcatc ggactcgggc
      721 tggcgattac tgaacgagcg gtaaacgctc acggaggaac cgtgcaggcg gcaaacctcc
      781 cggatgggtt tgaggttgct ttgaccttgc cactatcggc ttgaacatca caggcttcaa
      841 attaacgctt gccagatttg ggcaaagcgc cagacttcgt gaaaggtatt gtacagcggc
      901 actggcgcca cccggatgac gtttggttcg cggaaatctc caatcacccc ggcttcttcc
      961 agccgctgaa ataccgtgcg tgctccatca aaaacccgga ttgaaatctg gcagccgcga
     1021 tctttgaggt tacgtggagt aatgaccgcg atctttccac tctggttttg atcaagcaaa
     1081 gagagcagat agctggtcaa tttttcagat ttttcccgaa gtgccaggat tcctgcttcg
     1141 tgaaagatgt caagcgaggc tttgagggcc gccattgaca ggattggtag attgctcaac
     1201 tgccagcctg cggcaccggg ctgcaagtca aacgtaggcc gcatttcaaa gcgaatctgt
     1261 ggtttggtac cccaccaacc cgtaaaccgt ggcagttccg gagcatttcg atgacgctcg
     1321 tggacaaaac acccggccac cgcaccaggg cctgaattca ggtatttgta cgaacaccac
     1381 accgcaaaat caacgtccca gtcgtgcagg cgaagcggta catttccaac cgcatgtgcc
     1441 agatcaatcc ccaccacaca gccctgttta tgagccgcct ccgtgatcgc cgcaatatca
     1501 aaaacctggc cagtcaggta gttaacgccg gcaaacatca caagcgcgat tttgtgacca
     1561 tgctcttcga tcaatgcgac aatatcttca gtgtggagcg tgtcttcacc tggacgggga
     1621 tgagcttcga gaaaggccga ttccgggtca aatccatgaa accgaatctg actttggagg
     1681 gcatactggt ccgacggaaa agcattggct tcgagcagaa tctgatggcg ggttgcagtt
     1741 ggccgaaaaa acgagaccat caacaaatgc aggttcacgg tcaggccgtt catgatcgcc
     1801 acttcttcgg gctgggcgcc aaccagttcg gccattggtc caaggagggc ctgttcatac
     1861 gaaacccacg gattgtgctc tttaaagtgc ccgtggacgg ccagttgtgc ccaatcgtca
     1921 atttcctgca ggactgattc acggacacct ttgggctgca atccaagcga atttccacag
     1981 aaataaatca gcggttcgcc ggttacgctg ggttctggtt ggtgaaactg gtttcgaaaa
     2041 cgggcaagcg gatcggcctg atccagttgg agtgcaaagg cttcggttgg aagaaacata
     2101 agattttggg ctgaagacat cgggctgaag aagtcgggct gaagaaagcg ggctaagggc
     2161 tgaagacatc gggctgaaga atatcgggct gaagaatatc gggctgaaga aaatgggctg
     2221 aagaaaatgg gctgaagact tcgggctgaa gaatatcggg ctgaagaata tcgggctaag
     2281 ggctgaagac atcgggctga aggttattag gctgaagaac tggttttatt tcatccctca
     2341 tcccttcgat tgccccaagc ttgcgagtct tcaaccccaa gcccgatgtc ttcagcccga
     2401 agtcttcagc ccgctttctt cagccccgag ct
//
LOCUS       DAOQDO010000874         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38736_length_2429_cov_2.978517, whole genome shotgun sequence.
ACCESSION   DAOQDO010000874 DAOQDO010000000
VERSION     DAOQDO010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38736_length_2429_cov_2.978517"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PKZ53_13845"
     CDS             <1..950
                     /locus_tag="PKZ53_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41570.1"
                     /translation="STGERNKFMIIRGKYFALYLALVSLMCFSALKIDSVSGKTQLHQ
                     HLTAEIRDLSLKMVRQSAFHNEPIEVQFMKGKDGILAFNKQVRASDEWLSGFSIQVRN
                     TWNQPIRAMNLVIIVPSSAPQAGGDDEFRGVAQLSFGNTSDEKSRTEFDSVLYPSQAR
                     TLSLSPEAYQNFKSVLEMSGPISTVTRVFLSVQDVEFENGDVWLAGSMFAPEPGGRSW
                     IRKDLEGTKPYVAKASYASSPISRARRVDCGFLVRLEDARTCRSCGLQGQPYKSPTIS
                     YPGYGDVQTKSTTIRCVLQPVCESRITTYTACSSVINES"
     gene            1330..>2429
                     /locus_tag="PKZ53_13850"
     CDS             1330..>2429
                     /locus_tag="PKZ53_13850"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNJ41571.1"
                     /translation="MLTPAQAFGSGQDAPARVANGHKPVTKGNLKVVVLEGSPYNRGL
                     IHGRTLKKEIGEILQLWKADVEQTYKMKADEFTARFLKTPNFIPAIQKWTPELLDEVK
                     GISDGSGIDFNTILMLQLGDEQWAQGPDVTAEHCTSISVNKRGPNPTFVAQNMDIEPF
                     YQGYQTLLHIKDSQTSVESFVLTTPGLIGLNGLNNHSVAITCNTLLQLESSHDGLPVA
                     FIVRGVLEKTSFDLAVEFVKGIHHASGQNYIIGGTEKAVGIECSAKKTSQYVTDAGPG
                     ITYHTNHPLANDNYSTRYLERLKKLNRTVAEGAYYCYRFESLEKRFKHRSEVVGVDVI
                     KSALSSRDSKISPISNESTFGCTIMVLSDKPE"
BASE COUNT          682 a          533 c          575 g          639 t
ORIGIN      
        1 catcaaccgg agaaagaaat aaatttatga ttattcgtgg aaaatacttt gctttgtatc
       61 tggcgctggt gtccctgatg tgtttcagtg ctttaaaaat agattctgta tctggaaaaa
      121 cgcaactaca tcaacattta acggctgaaa tcagggattt gtcattgaaa atggtgcggc
      181 aatccgcatt tcacaatgaa cctatcgaag ttcaatttat gaaaggaaaa gacgggatac
      241 tggcctttaa caaacaggtg cgggccagtg atgagtggtt gagtggattt tccatccagg
      301 tcagaaatac ctggaatcag cccatccggg ccatgaatct ggtcattatt gttccttcct
      361 cagcaccaca ggctggcggg gacgatgagt ttcgtggggt ggcccagctt tcttttggaa
      421 atacatccga tgaaaaaagt cggactgaat tcgactcagt tttgtatccg tcgcaagccc
      481 gaaccctttc gttatcgcca gaagcttacc aaaatttcaa atcggtcctg gaaatgagcg
      541 ggccaatttc aactgtcacc agagtctttc tttcagtcca ggatgttgaa tttgaaaatg
      601 gagatgtgtg gcttgctgga agtatgtttg cgccagaacc aggcggtagg agttggattc
      661 gcaaagacct tgaaggtaca aaaccctatg tagcaaaggc cagctatgct tcttcaccaa
      721 tcagtcgggc caggcgggtt gattgtgggt tcctggttcg gcttgaagat gcacgtacct
      781 gccgtagctg tggattacag gggcaaccat ataaatcacc gactatttcg tatcccggat
      841 atggggatgt ccagacgaag tccactacga tcaggtgtgt attacaaccg gtttgtgaat
      901 caagaatcac tacatacaca gcttgtagct ctgttatcaa tgagtcctga accatctgag
      961 ctttcggtag cagctatcag ctattcctcg tttgattttt cacgactctt tggtacttcc
     1021 tgtggaaacg aacaaaactc caaaaatgaa tattgattct aaagaactta ccggtttgaa
     1081 ttttaatcaa aatgccaatt tttcacggca aataccaaag agcctttttc gcccaggtat
     1141 tcgctcatct tacaaccttc tggcagaacc tgattgacgc cgtggacctc aggtcagagc
     1201 gttctctggc aaaacaaaat gatggatgtt gcttgtgatt atccaggctc aaggatggtg
     1261 gttcagaaaa cgaggaggct tatgaaattt ctgatctttg tttgtagtct tattatagga
     1321 acaacaatga tgttgacacc tgctcaggca tttggaagtg gtcaggatgc gcctgcacgt
     1381 gtggcaaacg gacataaacc agtgaccaaa ggcaacctca aagttgtcgt gcttgaaggt
     1441 tcaccttaca accggggact gattcatggt cggacactga aaaaagagat tggcgaaatc
     1501 cttcaactgt ggaaagcgga tgtcgagcaa acctacaaaa tgaaagcaga tgaatttacg
     1561 gccagatttc tcaagacccc caatttcatc cccgccattc agaaatggac tccggaactt
     1621 ctggacgaag taaaaggcat ctcagacggt tcaggtatag atttcaatac aatattgatg
     1681 ttgcagttag gtgacgaaca gtgggctcaa ggaccggatg tgaccgccga gcattgcact
     1741 tcaatcagcg tcaataagcg cggtccaaac cctactttcg ttgcccagaa catggacatt
     1801 gaaccttttt accaggggta tcaaaccctg cttcatatca aagactcgca aacatcggtt
     1861 gaatcgtttg tgctgaccac acctggactg attggtctca atggcttgaa caatcattca
     1921 gttgccatta cctgtaacac cttgttgcaa ctggaatcct ctcatgacgg gttgccggtg
     1981 gcgtttatcg tgcgaggtgt tctggaaaag accagttttg acctggcggt tgaattcgtc
     2041 aaaggcatac accatgcgtc aggtcagaat tatattattg gcgggacaga gaaagcggtt
     2101 gggattgaat gttcggcgaa gaaaaccagc cagtatgtca ctgatgccgg accagggatt
     2161 acttaccaca ccaatcatcc cctggcgaac gacaattaca gcacccggta tctggagcga
     2221 ctcaagaagc tcaaccgaac agtcgctgaa ggggcatatt attgttatcg gtttgagtcc
     2281 cttgaaaaaa gatttaaaca caggtctgaa gtcgtaggcg ttgatgtgat caagtcagct
     2341 ttaagctcac gtgactcaaa aatatccccg attagcaacg agtcaacctt tggctgcaca
     2401 attatggtgc tgtcagacaa accagaatt
//
LOCUS       DAOQDO010000875         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38761_length_2428_cov_2.998736, whole genome shotgun sequence.
ACCESSION   DAOQDO010000875 DAOQDO010000000
VERSION     DAOQDO010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38761_length_2428_cov_2.998736"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..945)
                     /locus_tag="PKZ53_13855"
     CDS             complement(61..945)
                     /locus_tag="PKZ53_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNJ41572.1"
                     /translation="MQEFFRLLIGTVALRPYVFVFFAAYLFLAITSMGWRRTAVFTVL
                     AYVIAFVCEWSSVHNGFPFGLYYYIATTTNQELWVAGVPFMDSLSFTFLSYVSFEMAI
                     VLRSRLIVRPFDVQVVLTNRVRRSWFTTFVSALLMMYLDIVIDPVALQGEKWFLGKIY
                     YYPFGGSYFGVTIANFAGWFFVNAAIIRVYLMIERWLETWDRQGVWYYPFKGLSPAGL
                     YFGILIFNVSVTFWIGEITMGWASCFISATLLFLLVNHISQQPQPPVKTVIEEHLRDF
                     PESPLVPVYPQIFARGEL"
     gene            1211..1840
                     /locus_tag="PKZ53_13860"
     CDS             1211..1840
                     /locus_tag="PKZ53_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ41573.1"
                     /translation="MVKLQSQGDEVSPEHPAENHVPDESLVESVLNGDDSAFGVLFDR
                     HKRRMAQLAAKFFQRSERIEDVVQDIFTKLYFVLGEYSVREGSSFAAWLTRVATNVCY
                     DQLRSARRKPESNLLELTEDEADIVHQRIHQPHSSQHNLEHRLISHDLAKKLLARLDP
                     KDQMVLVLLEVEGLSITEIADVTGWSASNVKVRAHRARLALRKVVEDFV"
     gene            1873..2334
                     /locus_tag="PKZ53_13865"
     CDS             1873..2334
                     /locus_tag="PKZ53_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41574.1"
                     /translation="MFWRKKKSTVSDEALDRVSTEVLQALRDGVPESELDSLNLHYGQ
                     LRARIAEVKSQRSQSFFDFAAILTLAPRALTVMALITVFAMSAEFLVKPQGNVLPQTE
                     LAQSEQTFEENVVPITAPVTACSISTNPQCVVSTGDVWTLLMRQDTPLPTR"
BASE COUNT          657 a          646 c          593 g          532 t
ORIGIN      
        1 gatagtcata aatgagcgct ccaaacatca aaccaataaa ggcaattgtc accagccaga
       61 tcatagttca cctcgggcaa aaatttgtgg atacaccggc accaatgggc tctctggaaa
      121 atctcgaaga tgttcttcaa tcacggtttt gacaggcggt tgtggttgct ggctgatatg
      181 attgaccagc aggaacaaca acgtcgccga aataaagcaa ctcgcccaac ccatcgtaat
      241 ttcgccaatc cagaacgtga ctgacacgtt aaaaatcagg atgccgaaat aaagccccgc
      301 cgggctcaag cctttgaatg gataatacca gacaccctgg cggtcccacg tttccagcca
      361 tcgctcaatc atcagataaa cccggatgat agccgcattc acaaaaaacc agccggcaaa
      421 gttggcaatc gtgacgccga aataagagcc accaaaggga taatagtaaa ttttccccaa
      481 aaaccacttc tcaccctgga gcgccaccgg gtcaatcaca atgtcgaggt acatcatcag
      541 cagtgctgac acaaacgtgg tgaaccagga ccggcgaaca cgattggtca acaccacctg
      601 cacatcaaac gggcggacaa tcagccttga cctgagcaca atcgccattt caaagctgac
      661 ataagagaga aaggtaaagg aaagcgaatc cataaacggc acgcccgcga cccaaagctc
      721 ctggttggtt gtggttgcga tatagtaata caacccgaag ggaaacccgt tgtggaccga
      781 actccattca cagacaaagg caatcacgta ggccagcacg gtaaagaccg ctgttcgccg
      841 ccagcccatt gaagtgatag ccaaaaacag gtacgcggca aaaaaaacaa acacataggg
      901 ccgcaaagcg actgttccga ttaacagccg aaaaaattcc tgcatacttt ttgaaaaggg
      961 cttctttctt ggaatttaaa acaggtaagc tgaagcggtt ggaattgatt tccaaaaaat
     1021 ttgcgaacca gctaacttgt tgatttcaac tactttgctg attttattct gtttttgcaa
     1081 gggaaagctt caccctgaaa ctttttcctt ttccgctgag cgtgtaacca cgcggctgga
     1141 tggagcgtta acccctttgc actcaatacc agatccttct tttcctgaat cgttcaaaaa
     1201 ctcgatgcca atggtcaaac tccaatcaca gggggacgag gtgagcccag agcaccccgc
     1261 cgaaaaccat gttcccgatg aatcgctggt tgagtcggtc ctcaacggtg atgacagtgc
     1321 ctttggcgtc ctgtttgacc ggcataaacg ccggatggcc caactggcgg caaagttctt
     1381 tcaacgctca gagcggatcg aagatgtggt gcaggatatt ttcaccaagc tctattttgt
     1441 gcttggggaa tattcggttc gggaagggag ttcctttgcc gcctggctga cccgtgtggc
     1501 caccaatgtc tgctatgacc agcttcggtc tgcacggcga aaaccagaaa gcaacctgct
     1561 ggaactcacc gaagacgagg ctgacattgt ccatcagcgc attcatcagc cgcattcctc
     1621 ccagcacaac ctggagcatc gcctgatctc gcacgatctg gccaaaaagc tgcttgcccg
     1681 gctcgatcca aaagatcaaa tggtgctggt actgctcgaa gtcgaaggac tttcgatcac
     1741 tgaaattgct gatgtgactg gctggtcagc gtccaatgtg aaagttcgtg cccaccgggc
     1801 gcgtcttgcc ttgcgcaagg tggtcgaaga ttttgtgtga cgggttggct ctcaacccac
     1861 gaggtgtgat ctatgttttg gcgaaagaaa aaatcaaccg tgtctgatga agcccttgac
     1921 cgggtgagca ccgaggtact gcaggcactt cgagacggcg tcccagaaag cgaactggat
     1981 tctctcaacc tccactatgg tcagcttcgc gcccggattg ctgaagtcaa aagccagcgc
     2041 agccagtcct tttttgattt cgcggcgatc ctgaccctgg cgccacgggc actgaccgtg
     2101 atggccctga ttaccgtttt tgcgatgagt gcggagtttc tggtgaaacc ccagggaaat
     2161 gtgcttcccc aaactgaact ggctcaatca gagcaaacct ttgaagaaaa tgtcgtgccg
     2221 attacggcac ccgtcacggc ctgctcaatt tcaacgaatc cacaatgcgt ggtttcaact
     2281 ggcgatgtct ggacactcct gatgcgccag gacacgccac ttccgactcg ataataccat
     2341 ttggggcttg gggcttgggg ccatcgaagg gatgagggat gagggatgaa agaaaaccac
     2401 ttcttcagcc cgacttcttc agcccgac
//
LOCUS       DAOQDO010000876         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38783_length_2427_cov_10.601180, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010000876 DAOQDO010000000
VERSION     DAOQDO010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38783_length_2427_cov_10.601180"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKZ53_13870"
     CDS             <1..460
                     /locus_tag="PKZ53_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNJ41575.1"
                     /translation="IGAVKVEVLSPPSVSEPLFQSDNDNSLVLRISYGQHSFVFTGDI
                     EKAAEHWLVSQSNLQSCTVLKAPHHGSKTSSTVEFLEHTRPQHVVFCAPEHSIYHHPH
                     PDVVRCYQRLLPTTHLWQTGLNGAVTFETDGVWLSATGFIDSLPTELPKP"
     gene            complement(446..916)
                     /locus_tag="PKZ53_13875"
     CDS             complement(446..916)
                     /locus_tag="PKZ53_13875"
                     /inference="COORDINATES: protein motif:HMM:NF020177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HNJ41576.1"
                     /translation="MPLINLTSELIQILSTAGIGLLVGALLTEGCLLVPYWRSLSADR
                     FHALYRDLHPLLYRYFTPLTVAPLLLSLIAGVISMLVAGSKSWPTLTAGVLMVCVAAT
                     HEIYFKKANSQFATGSLNPNELSNELARWSAWNWFRVGLGLMAFGASMLGLWLG"
     gene            1139..2236
                     /gene="serC"
                     /locus_tag="PKZ53_13880"
     CDS             1139..2236
                     /gene="serC"
                     /locus_tag="PKZ53_13880"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNJ41577.1"
                     /translation="MSSPVPKRIFNFSAGPAIMPVSVLEQIRDEMMSLPGVGMSVLEI
                     SHRSKVFDEIIQTAEAKLRSLMGIPDNYHVLFLQGGASLQFSMVPINLLPANGSADYI
                     VTGVWSEKAVKEAKKVGTVKTAGTTKAENFSRIPNQSELTLDPDAAYVHFTSNNTIFG
                     TEWRTEPEVGNVPLICDASSDILSRPVDVSKYGMIYAGAQKNIGPSGVTLVIVRDDLL
                     AKIPDGLHTMLDYRTHVKDKSLYNTPPTFGIYVIGLVAKWLEGLGGLVAMEKMNQEKA
                     GILYDAIDASEFYRGTAEKNSRSLMNVCFRLPSEELEKDLIKQATAAGFDGLKGHRSV
                     GGLRASIYNAFPKEGVAALVEFMKEFERKHA"
     gene            complement(2233..>2427)
                     /locus_tag="PKZ53_13885"
     CDS             complement(2233..>2427)
                     /locus_tag="PKZ53_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41578.1"
                     /translation="RVQGLKTRVQGLKTRVQGLKTRVQGSGFRVFDKTIQDTPSKTRN
                     PEPGTRNPEPGTRNPEPFLP"
BASE COUNT          587 a          637 c          618 g          585 t
ORIGIN      
        1 catcggggcg gtgaaggttg aggttctttc gcctccatct gtatctgaac cgttatttca
       61 gtctgataat gataattctt tggtgcttag aatctcctat ggccagcact cttttgtatt
      121 cactggcgat attgaaaaag cggctgagca ctggctggtt tcccagtcga atctccaatc
      181 ctgcaccgtt ctcaaagctc cacaccacgg cagcaaaacc tcaagcacgg tcgagttttt
      241 agaacatacc cggcctcagc acgtggtgtt ttgcgccccg gagcattcga tttaccatca
      301 ccctcatccg gatgtagttc gttgctacca gcgcctcctc ccaaccaccc acctctggca
      361 gactggtctg aatggtgcgg tcacgtttga aaccgatggg gtctggcttt ccgccacagg
      421 gtttatagat tctttaccga ctgagctacc caagccatag cccaagcatt gaagcgccaa
      481 acgccatcaa tcccagacca acccggaacc agttccaggc tgaccagcgg gctaattcat
      541 tggaaagttc attggggttg agtgaaccgg tggcaaattg cgagttggct tttttgaagt
      601 aaatttcatg cgtggcggcc acacatacca tcagaacccc tgccgtcagt gtgggccagc
      661 ttttggaacc ggcgaccagc atgctgatga cgcctgcaat aagagaaagg agcaacggcg
      721 caacggtcaa tggtgtaaag taccggtaga gcaggggatg gaggtctcga taaagcgcat
      781 gaaagcgatc tgcggagaga ctgcgccagt atggcaccag cagacaccct tcggtcagca
      841 acgcacccac cagcaggcca atgcctgccg ttgacaggat ttggatcaac tcgcttgtca
      901 ggtttatcag aggcatttgg aaacacctga atgccacacg aacaggattt tggtttcacc
      961 taatgtagtc atgcgggaga ttcacttcaa ccgaattccc aaaaccggag agaagatttt
     1021 ctcagtccac gtgattgccc ttgattcttc gtttcagact tgaatactct ctgctttccg
     1081 caaattttaa ccttcactca tcacttcatt tttaagtctt tcgaaggagt aaacttccat
     1141 gtcgagtcca gtaccaaagc gaatttttaa tttcagcgcc ggcccggcca tcatgcctgt
     1201 gtccgtgctg gagcaaatcc gtgacgaaat gatgtcgttg cccggcgtcg gcatgtccgt
     1261 tctggaaatc agccatcggt caaaagtgtt tgatgaaatc attcaaaccg ccgaggccaa
     1321 actccgcagc ctgatgggga tccctgacaa ttatcacgtg ctgtttttgc agggcggcgc
     1381 cagtttgcaa ttctcaatgg tgcccatcaa cctgctgccg gccaacggtt ctgccgacta
     1441 catcgtaacc ggcgtctggt cagaaaaagc ggtcaaggaa gccaaaaagg tcggcacggt
     1501 gaaaacggct ggcaccacca aagccgagaa tttttcgcgt atcccaaatc agtcagaatt
     1561 aaccctcgac ccggatgccg cctatgtgca tttcacctcc aacaatacaa tttttggaac
     1621 tgaatggcgc accgagcccg aagtcggcaa cgtgccgctg atttgtgacg cgtcgtcaga
     1681 cattctgagc cgaccggttg atgtttcaaa gtatgggatg atttatgctg gcgcccagaa
     1741 aaacattggt ccttcaggtg ttacgctggt cattgtgcgc gatgacttgc tggcgaaaat
     1801 tcctgatggg ttgcacacca tgctcgacta ccggacccac gtcaaagaca aatcgctcta
     1861 taacacaccg ccaacctttg gaatttatgt gattggactg gtggcaaagt ggctggaagg
     1921 gctgggcgga ctggtggcga tggagaaaat gaatcaagaa aaggccggca ttctctatga
     1981 tgcgattgat gcctctgagt tctatcgcgg aacggctgaa aagaacagcc gctcactgat
     2041 gaatgtctgc ttccgtctgc caagtgaaga gcttgaaaaa gacctcatca aacaggcaac
     2101 cgccgctggg tttgatgggc tgaaaggcca tcggtcagtc ggcggtctgc gggcttcgat
     2161 ttacaacgcg ttcccgaaag aaggtgtcgc cgcgctggtt gaatttatga aggaatttga
     2221 gcggaaacac gcttagggaa gaaagggttc cgggttccgg gttccgggtt ccgggttccg
     2281 ggttccgggt tccgggttcc gggttttcga aggagtatcc tgtatcgttt tgtcaaaaac
     2341 cctgaaccct gaaccctgaa ccctggtttt caaaccctga accctggttt tcaaaccctg
     2401 aaccctggtt ttcaaaccct gaaccct
//
LOCUS       DAOQDO010000877         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38802_length_2427_cov_2.020236, whole genome shotgun sequence.
ACCESSION   DAOQDO010000877 DAOQDO010000000
VERSION     DAOQDO010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38802_length_2427_cov_2.020236"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKZ53_13890"
     CDS             <1..856
                     /locus_tag="PKZ53_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41579.1"
                     /translation="TAPDYICGGCGAVLCKGCVNSVASGAICTLCGQLCQKYAEVTKQ
                     MQRQAFQKSGFGMEDLIQSLTYPLKHFVMFAVAAVAITAFGFIHDGLANLMMVGYISI
                     IIRRIADGRVDEAVLLDFEDMRGVGFLGISIWLIIFGPAVVIRTTGIDETIGMSLGAA
                     PLFLWPFSFGLLGVLAILWAVFYYPMALLVAGFSQSFGAVLNPLVGLDTIKRMGGVYV
                     KVWLMYFGIEVVFVILKVVGAITGILGFPIVGYVVFKPLEFFFTSMIACVLGLSIFKC
                     ADRLGVHV"
     gene            898..2313
                     /locus_tag="PKZ53_13895"
     CDS             898..2313
                     /locus_tag="PKZ53_13895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ41580.1"
                     /translation="MNYRQQETFLNQYAQVGVGMIALNLVFFGVLVNVTRRLLSWGDI
                     TKLTLLAIALSVAVFYVPKYLFLTIEEHKKYSWTIRIRWILGGLFLLSTPLVGRSFKD
                     YGLMVGAAGFVLSTNWAVREVFRQVQRKQKKPELLAFIPFVYAAGDSLGLGLLLSNKV
                     LTLETGVLLTAMAVHLLILLLSQKQLALIPVIVMGVAGLLWFVGGQGPHIGSVTSWMI
                     VSLALCWMARDRHRKNLEEMLFSLVDFTGETPEHIQEALLTSTGALAANWNANPPTTP
                     EELETWYRQNSGYYIYDLAQFHLAYKHIVFTLDVISQARGRVLDYGAGIGDLSLELAA
                     RGDRDVTYFDVDGETQQFARWRARERKLRVNFASDRRRLESQYDTIILLDVLEHLADP
                     VETLEFLVNRLAPGGKLVASIAFGPTKAHPMHFDHDFDAKTWLRERGFFDAKTVSLRL
                     FGSEAMRRSGVIIYQKKWTPQ"
BASE COUNT          518 a          539 c          669 g          701 t
ORIGIN      
        1 aacggcgcca gactatatct gcgggggatg cggtgccgtc ttgtgcaaag ggtgtgttaa
       61 ctctgttgca tcaggggcga tttgtacact gtgtgggcag ctatgtcaga aatatgctga
      121 agtcacaaag caaatgcagc gccaggcttt tcagaaatct ggctttggga tggaggatct
      181 gattcaatcc ctgacatacc ctttaaaaca ttttgtgatg tttgccgttg cggcagttgc
      241 gattacagcg tttgggttta tccatgatgg tctggctaat ctcatgatgg ttgggtatat
      301 ctcaatcatc attcgacgaa ttgctgatgg tcgcgtagat gaagccgtac tgctggattt
      361 tgaagatatg aggggtgttg gctttcttgg tatcagtatc tggctgatta tatttgggcc
      421 agccgtcgtc attagaacaa ctgggataga tgaaacaatc ggaatgtcgc tgggagcggc
      481 cccattgttc ctgtggcctt tttcatttgg gttgttgggg gtgcttgcaa tcttgtgggc
      541 tgtgttttat tacccgatgg cattacttgt ggcagggttt agtcagtctt ttggggcagt
      601 cctgaaccca cttgtcggac ttgataccat taaacgaatg ggcggcgtgt atgtcaaagt
      661 ttggttgatg tattttggaa ttgaagtggt ctttgttatt ctcaaagtgg tgggtgccat
      721 aactggaatt ctggggtttc cgattgtcgg gtacgttgtt tttaaaccac ttgaattctt
      781 tttcacttca atgattgcct gcgtgttagg gctttcaatt ttcaaatgtg ctgaccgcct
      841 gggtgtccac gtctaattta tgggatatct gctggttggc taacctctaa agtcactgtg
      901 aattatcgtc agcaagaaac atttctgaat caatatgcac aggttggggt cggaatgatc
      961 gctctcaacc ttgttttttt tggggtcctg gtcaatgtca ctcgccgatt gttgagctgg
     1021 ggcgacatca ccaaactgac actgctcgcc attgcactca gtgtcgctgt tttttacgtc
     1081 cccaagtatc ttttcctcac gattgaagag cacaaaaagt attcctggac tatccggatc
     1141 cgatggattt tgggcgggct gtttttgctt tcaactccgc tggttggccg gtcattcaag
     1201 gattacgggc tgatggttgg cgcggcagga tttgtgttat caaccaactg ggcggtgcgc
     1261 gaggtctttc gacaggtcca gcgcaaacaa aaaaagccgg agttgcttgc cttcatcccc
     1321 tttgtctatg cggctggtga tagccttggc cttggattgc tcctcagcaa taaagtcctg
     1381 actcttgaaa ccggggtact gttaacggca atggccgttc atttgctgat tttgctctta
     1441 tctcaaaagc agttggccct gattccggtg attgtgatgg gggtcgcggg actcctctgg
     1501 tttgtgggag ggcaggggcc acacatcggt tccgtgacct cctggatgat tgtctcgctg
     1561 gctttgtgct ggatggcgcg ggaccgccac cggaaaaacc tggaagaaat gttgttttca
     1621 ctggtggatt ttactggtga aactccagaa cacatccagg aagcgctgtt aaccagcacc
     1681 ggggctttag ccgcaaactg gaatgccaat ccgccaacca cccctgagga actcgaaacc
     1741 tggtaccggc aaaattcagg ctattacatc tatgacctgg cccagtttca cctggcatac
     1801 aagcacattg tgtttacgct ggacgtgatc tctcaggccc gagggcgggt gctggattat
     1861 ggtgccggca tcggagattt atcgctggaa ctggccgctc gcggagaccg ggatgtgacc
     1921 tactttgatg ttgacgggga aacgcaacag tttgcccgct ggcgtgctcg tgaacgaaag
     1981 ctgcgagtta actttgccag tgatcgccgc cgtcttgaga gccagtatga caccattatt
     2041 ctgctggatg ttctggaaca tttagccgat ccggttgaaa cgctggaatt tttggtcaat
     2101 cggctagccc caggagggaa actggtggct tcaatcgctt ttggcccaac caaagcacac
     2161 cccatgcact ttgatcatga ctttgacgct aaaacgtggc tccgggaacg gggatttttc
     2221 gatgccaaaa cggtgtcact tcgacttttt ggctctgaag ccatgcgccg ctctggcgtg
     2281 atcatctatc agaagaaatg gacgccacag tgaaggggaa tgaagaaaat accgggttcc
     2341 gggttccggg ttccgggttc cgggttttcg aacccaggaa cttttttcat tcttcatagg
     2401 gcttacgcag agaaagtaag ctattaa
//
LOCUS       DAOQDO010000878         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38870_length_2424_cov_3.746306, whole genome shotgun sequence.
ACCESSION   DAOQDO010000878 DAOQDO010000000
VERSION     DAOQDO010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38870_length_2424_cov_3.746306"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="PKZ53_13900"
     CDS             complement(<1..1632)
                     /locus_tag="PKZ53_13900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ41581.1"
                     /translation="MSFSKLSAGILALCLLLPYGALFSPSVQAGPTTHPRFLIFQTTT
                     GTLLGLVLDQQSNPIMGSLVRFENTANGLARVARTDAQGQYRFDLVPPGIYNISASRE
                     GYQPNAILNYVVEVNREKLVKPPPIKLLPESAPPAPPPPPSDPTNPTQGIQANTADST
                     LRGSATSEFVSSLPLPGIRSFDVFALLSPGVAPPPATYGANGPGIGSGVGTAGQFSVN
                     GNRARANNFTIDGSDNNDQDVGVRRQGFTPAIPQTVESISEFQMSTLLADAEAGRNTG
                     GQVNVVSKSGTNQVHGSMYDLLTDSSLIARDYFDLTGGPSGKENSFTRNQFGGVLGGP
                     IIKNKLHFFAAFEKQDVNRSQETHFSVPTLGQRTAGLAFGQRFNPNGTRLGRDVFSFY
                     PLPNNTGGPYGANTYTEILPGSADGVVFSAKVDYQFKGFGKDMTFGSRYNITDDETRI
                     PTVGGALRSAITAQTRTQNLAFEHDTQLNDRDYNQIRFSFGRTALGFDEVPGNPFLFG
                     RGTQLGPVGRIVLSPFSSAGVDPFTFPQGRANNTFQ"
     gene            complement(1789..2310)
                     /locus_tag="PKZ53_13905"
     CDS             complement(1789..2310)
                     /locus_tag="PKZ53_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41582.1"
                     /translation="MSSYADPRSQVKAPAICLLVLGILNILIGGFYTLSSFAGALMKM
                     DQTVFSSEAEEIGYYFGRYGATGIAVFSVLFAPLIIVGAVKMMNLSGYTLAKTAAILT
                     IVPLTSCCCLFTVPFGIWAFLVLGKPEVKAAFLNPSQNSPGGGYGSGGYGGGTQPYGG
                     APPYGGPPGGYNR"
BASE COUNT          634 a          617 c          640 g          533 t
ORIGIN      
        1 ctggaacgtg ttgttggccc ggccttgcgg gaaagtaaac gggtcaactc cagccgaact
       61 aaacggcgag agcacaatcc ggccaaccgg ccccagttgt gttccccgac caaagaggaa
      121 cggattgccg gggacttcgt caaacccaag cgccgtacgc ccaaaggaaa accgaatctg
      181 gttgtaatcg cgatcattga gctgggtatc gtgttcaaaa gccagatttt gcgtccgggt
      241 ctgggcggta attgcagaac gaagggcgcc gccaacggtt ggaatccggg tttcgtcatc
      301 ggtaatgttg taccggctgc caaaggtcat gtcttttcca aaccctttga actggtaatc
      361 aaccttggct gaaaacacca caccatctgc cgatccaggc aaaatttcag tgtaagtgtt
      421 ggcaccatat ggtccgcccg tgttgttcgg aagtggataa aatgaaaaga catcgcgtcc
      481 caggcgcgtc ccattcggat taaatcgctg accaaaagcc aacccggcag tgcgctgacc
      541 cagggttgga actgagaaat gcgtttcctg tgaccgattg acatcctgct tttcaaacgc
      601 ggcgaagaaa tgcagtttgt ttttgataat cggtccgccc aggactccgc caaactggtt
      661 gcgggtaaag ctgttctcct tgcctgatgg tccaccagtc aggtcaaagt aatctcgggc
      721 aatcaatgac gagtcagtca gaagatcata catactgccg tgaacctgat tggtccctga
      781 tttggacacc acgttgacct gaccgccggt gttacgcccg gcttcggcat cggccagcaa
      841 agtggacatt tgaaattcac taatgctttc aacggtttgc ggaatcgccg gcgtaaatcc
      901 ttgccgacga acaccgacat cctggtcatt gttgtcagaa ccatcaatcg tgaagttgtt
      961 ggctcgcgcc cggtttccgt tgaccgaaaa ttgtccggca gtgccgacac cggaaccgat
     1021 tccggggcca ttggcaccat aggttgccgg cggtggcgca acacccggtg acaacaaagc
     1081 aaaaacgtca aagctccgaa tgccaggcag cggcagtgat gaaacaaatt ctgacgtggc
     1141 actcccgcgc agagtgctat ccgccgtatt cgcctggatt ccctgggttg ggttggtcgg
     1201 gtcagacggc ggtgggggtg gcgccggtgg cgccgattct ggaaggagct tgatgggtgg
     1261 tggcttgacg agtttttcgc ggttgacttc gaccacgtaa ttcaaaatcg cattgggctg
     1321 gtagccttcg cgggaagcac tgatgttgta aatccctggt ggcaccagat caaaccgata
     1381 ctgcccctgt gcatcggttc gagccacacg agccagaccg ttggccgtat tttcaaaccg
     1441 cacgagcgat cccataatcg ggttgctttg ctggtcaagc accaatccca gcagtgttcc
     1501 ggtggtggtc tgaaaaatga ggaaccgagg gtgagtggtt ggccccgcct gcaccgaagg
     1561 tgaaaaaaga gcaccatacg gcaagaggag acaaagggcc agaatcccgg cactgagctt
     1621 tgaaaaactc ataaacggac tccttatttg tttaaaaaca aactgaatac ctggttcaaa
     1681 tagagaaaag gcgaggaaaa gaatcttgaa agctggaacc gagcttaaac cagagtgagc
     1741 cgaaagtaca actggcggga aaaacccgcc agttgattca caatgatttt atcggttgta
     1801 tccgccaggt gggccaccat acggaggcgc tccgccatag ggctgggtgc cgccgccata
     1861 tcctccgctg ccatatcctc cgccagggga gttttgggat gggttcaaaa atgcagcttt
     1921 cacctcaggt ttacccaaca ccagaaaagc ccaaatccca aatggaacag taaataagca
     1981 acagcacgag gtcagtggaa caatcgttaa aattgccgct gttttggcca gggtgtaccc
     2041 tgacaggttc atcattttca ctgccccgac aatgatgagt ggggcaaaca agacactaaa
     2101 aacagcgatc ccagttgccc cgtatcgccc aaagtaatat ccaatttctt cagcttcaga
     2161 agaaaaaacc gtctggtcca ttttcatcag cgcgccagca aatgacgaaa gtgtgtaaaa
     2221 cccaccaatc aaaatgttga gaatcccaag caccagcaaa caaatagctg gagctttgac
     2281 ctgacttcga gggtcggcat aagacgacat acggatctcc ttcaggattc aaaaattgag
     2341 aatcaaaaag ctataagtac cttgtcaaaa gtttcctgag cgattgaatg ttggaagtgg
     2401 ttggtttttt tcgtgtattt cgtg
//
LOCUS       DAOQDO010000879         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38921_length_2422_cov_3.375581, whole genome shotgun sequence.
ACCESSION   DAOQDO010000879 DAOQDO010000000
VERSION     DAOQDO010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38921_length_2422_cov_3.375581"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKZ53_13910"
     CDS             <1..807
                     /locus_tag="PKZ53_13910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ41583.1"
                     /translation="MVTYLKVKSDKKDKTIAQKALEEIPVSSDLWASNPYLMLGALEQ
                     AGWKDRQEQYIQTILEKGASSNFKAAALFSGLSDADEAENEPLTRQYYDRLMKDFGDT
                     QMAQMAKMRFNPDRKVKAGQPLPAFLIKDIENQQVAYSNDSIKGKIYLIDFWAVWCGP
                     CVGEMENLHKAYDKFKGKNFEILSLSFDQKPEDVKKFREEKWKMPWKHAFVEGNFRSE
                     LAKLFEVNGIPKPILVDETGKIIAIEGKLRGETLEQTLGKVFADRQNAPK"
     gene            complement(966..2126)
                     /locus_tag="PKZ53_13915"
     CDS             complement(966..2126)
                     /locus_tag="PKZ53_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNJ41584.1"
                     /translation="MNEPTKHLGDILIVDDNPINLNLLSGMLAEHKYRVRVATSGKRA
                     LAAVRSCLPDLILLDITMPEMDGYEVCRQLKADPQTRDVPVIFISALDETMDKVKAFQ
                     IGGVDYVSKPFQFEEVLVRIENQLKISRLQHEMVQKNAELENANLKLKELDRIKANFT
                     AMLVHDLKSPLSVVKGTFEMFALEETLTEQGQILLTSADRSVDKILDLINEVLEVFRS
                     EAQDLTLSCKVVDPTQILQAAVEEIRLAATSQNISLTVNFGPNLPKVPVDVGKIERVF
                     SNLLSNAVKFTPPGGSISVDTEVSRGTGVESGSTFLVVNITDTGEGIPADVLPYIFDP
                     YQQATTKRSKLGVGLGLAIVKRIVAAHGGNVSVRSKVGVGSCFTVTLPTAGN"
     gene            complement(2229..>2422)
                     /locus_tag="PKZ53_13920"
     CDS             complement(2229..>2422)
                     /locus_tag="PKZ53_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41585.1"
                     /translation="APVSSQPPLRGEGMSTEPNHFLHSQFLILNSSFFIPSSFMPLLL
                     TGNLLQRNLDCEKRGLHRK"
BASE COUNT          652 a          567 c          617 g          586 t
ORIGIN      
        1 atggtgacct atttgaaagt aaaatctgac aaaaaggata aaacgattgc tcagaaagct
       61 cttgaggaga tcccggtaag ttccgacttg tgggcgagca atccatactt gatgttgggg
      121 gcccttgaac aggctggttg gaaagaccgc caagaacagt acattcagac aattttggaa
      181 aagggcgcca gctcaaactt taaagccgct gccctgtttt cgggcctgtc tgatgcggac
      241 gaagccgaaa acgaaccgtt gactcgtcaa tattatgatc ggcttatgaa agattttggc
      301 gatacccaaa tggctcaaat ggccaaaatg cggttcaacc cagatcgcaa agtcaaagcc
      361 ggtcagccac ttccagcctt tctgatcaag gacattgaaa atcaacaggt tgcatactca
      421 aatgactcca tcaaaggcaa aatttatctg attgattttt gggcagtctg gtgtggcccc
      481 tgcgttggag aaatggaaaa cctgcacaaa gcctatgaca aattcaaagg caaaaacttt
      541 gaaatcctga gcctgtcctt tgaccagaaa ccagaagacg tcaaaaaatt ccgcgaagaa
      601 aaatggaaaa tgccctggaa acacgccttt gttgagggca atttccgaag tgagttagcc
      661 aaactttttg aagtcaatgg gattccgaag ccaatcctgg ttgatgaaac tggcaaaatc
      721 atcgccattg aaggcaagct ccggggtgaa acccttgaac agacgttggg caaagtgttc
      781 gctgaccggc aaaatgcacc gaagtagggc tgaaaaaaac cagggctgaa gacgacgggc
      841 tgaagaaaat gggcttggga tttaagattg acccgttttc ttcagctccc agccccaagc
      901 cctcagcccc aagcccgatg tcttcagccc tcagccccaa gcccgatgtc ttcagcccga
      961 agttttcagt ttccagccgt tggcagggta acggtaaagc acgatccgac accaactttg
     1021 ctgcggactg acacattgcc accgtgggcg gccacgattc gtttgacaat ggccagaccg
     1081 agcccgacgc cgagttttga gcgtttggtg gtcgcctgct ggtatgggtc aaaaatataa
     1141 ggcaagacat ctgctggaat accttcgcct gtgtcagtga tgttgaccac caggaaggtt
     1201 gacccagatt cgacccccgt gccgcggctg acttcggtat caactgaaat tgagccgccc
     1261 ggaggggtga atttcacggc attggacagc aggtttgaaa acacccgttc aatttttccg
     1321 acatcaaccg ggactttggg cagatttgga ccaaaattga ctgtgagcga aatattctga
     1381 ctggtcgccg ccagccggat ttcttcaact gccgcctgta aaatctgggt gggatctacg
     1441 actttacagc tcaaggtcag gtcctgggct tcggatcgga agacttcgag gacttcgttg
     1501 atcagatcca ggattttatc cacgctgcgg tcagccgaag ttaagagaat ctgcccctgt
     1561 tctgtgagag tttcttccaa cgcaaacatc tcaaacgtgc ctttgaccac actcaacggg
     1621 gatttgaggt cgtggaccag catcgccgta aagttggctt tgatgcggtc cagttccttg
     1681 agtttgaggt tggcgttttc gagttcggcg tttttctgca ccatttcgtg ttggagtcgc
     1741 gaaattttaa gctgattttc gatgcgaacc aggacttcct caaattgaaa tggttttgag
     1801 acgtaatcca ccccgccaat ctggaaggct ttgaccttgt ccatcgtctc gtcgagcgcg
     1861 ctgataaaaa tcactggcac atcgcgcgtt tgcgggtcag cttttaactg gcggcagact
     1921 tcatacccat ccatttccgg catggtgata tcgagcagga tcaaatccgg gagacagctt
     1981 cgcacggcag ccagggcgcg tttcccgctg gtggccaccc gaacccggta cttgtgttcg
     2041 gcgagcatgc ctgataacag gttcaaattg atagggttat cgtcaacaat cagaatgtcg
     2101 ccaaggtgtt tggtcggttc attcatgcca gaaaggtcct atgtgtgaag aaatgaagaa
     2161 accaagaatg aagaatgaaa gatgaaaaat ccatagagga gtgttcaaac ctcaaaaaaa
     2221 ctttcaggtt actttctgtg taatcctctt ttttcacaat ccagatttct ttgcaacaag
     2281 ttaccggtca gcagtaaggg cataaaagac gatggaatga agaatgaaga attgagaatg
     2341 aggaattgag aatgaagaaa gtggttaggt tctgttgaca ttccttcgcc gcgtagcggt
     2401 ggctgacttg agaccggtgc tt
//
LOCUS       DAOQDO010000880         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38960_length_2421_cov_3.002113, whole genome shotgun sequence.
ACCESSION   DAOQDO010000880 DAOQDO010000000
VERSION     DAOQDO010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38960_length_2421_cov_3.002113"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKZ53_13925"
     CDS             complement(<1..932)
                     /locus_tag="PKZ53_13925"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ41586.1"
                     /translation="MLKPGRKLFSTFSWLTCGILLAGGTIATLRPVAANSAARSPLPE
                     AAPMVQQVAPLEFFPVPETGAVTFRAQTAKGPDFYLLDGRDLPEGSSVEAFRASSSNS
                     AQPLAMITADINGDGSPDILTSYGASDSRGYLTFRAQDGQGQFSETKVSSLPYTAPIA
                     FRVGDFNADGLADLAVVNANTNKLSLVAGDGQGNFSALTDLSVGKASTSLVVADLNHT
                     GLPGIAVGDASGEVRVIARKDSSFTYEATEAQILKAGAGVVALEAADVNADQRTDLVV
                     ATKAGVEIWVGDGAGKFSRQTTLASSEAVTAMALR"
     gene            complement(1612..>2421)
                     /locus_tag="PKZ53_13930"
     CDS             complement(1612..>2421)
                     /locus_tag="PKZ53_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNJ41587.1"
                     /translation="PFKPCSTIKLLTSLAALREGIADPDTPLAFGRTQFTMTQALARS
                     NNEYFQDLGEQIGLDRMLAYAREVGFGRPTEVNVPGESTGYLPTPANNLRRIFSHGDG
                     FGITAVQLAVFTAAIANGGTVYKPQILRTQDDIKHFKPEVAGTISLPEAARAKMIEGM
                     LAAVDYGTARRSNAASLAVAGKTGSCDCYCAQGTKLGLFTSFINPVDPSLVISVITTG
                     SRQRGSLASIVAGNIYNGLAETGGLAAAPRSAPLRVGRDAKGNLLLTNQQK"
BASE COUNT          594 a          640 c          619 g          568 t
ORIGIN      
        1 cgcaaggcca tcgccgtcac cgcttcactt gaagccagtg tggtttggcg tgaaaacttg
       61 cctgcgccgt cacccaccca gatttccaca ccggctttgg tggcaaccac caaatcggtg
      121 cgctggtcag cattcacatc agccgcctca agggcaacca ccccagcccc agccttcaga
      181 atctgggctt cggtggcttc ataggtaaag gaggaatctt tgcgggcaat gacccgaact
      241 tcacccgagg catcccctac ggcaatccct ggcagtcccg tatggttgag atcggccacc
      301 accagtgagg ttgaagcctt gccaaccgaa aggtctgtca atgcgctgaa gttcccctgc
      361 ccatcgccag ccaccaggga aagcttgttg gtgttggcgt ttacgaccgc caggtcagcc
      421 aggccgtcag cattgaaatc cccaacccga aaggcgattg gggcggtgta tggcagtgat
      481 gaaactttcg tttctgaaaa ctggccctgg ccatcttgtg cccggaacgt caggtacccg
      541 cggctgtcag atgcaccata actggtcaaa atgtcaggac tgccatcacc gttgatgtca
      601 gccgtgatca tggccagcgg ctgggctgaa ttgctggatg aagcccggaa cgcctcgact
      661 gaacttcctt cgggcagatc tcgaccgtca agtaagtaaa agtcggggcc tttggcggtc
      721 tgggcgcgaa atgtcaccgc tccggtttcg ggaacgggga aaaactccaa tggtgcaact
      781 tgctgaacca tcggagctgc ttcaggcagc ggactccgcg ctgcactgtt ggcggctacg
      841 gggcgaaggg tggcaatcgt gccaccagcg agcaggattc cgcaggtcag ccaactaaaa
      901 gtgctgaaaa gtttccttcc tggtttcaac attctggtcc tcctctatag gttggaacgc
      961 tctgaatgcg tcacatctct ttctcaagct ttgattgttt cgtctttaag caaaaaaact
     1021 tcaaaatgac agggttcttc agacaaaaga agctgaacgg gagagttcat gactcactca
     1081 tcatgtaaag actgtctttt ttggaaaaac ttctgattgg aaagtaaaag tgtgtagttt
     1141 ttgggttgtt actctggcgc agactttcct ctgcatttaa cgtgccatag tttttacttc
     1201 gcaccacgtc ccttgctttt ttgatctggg cttagaaatt actgcactta tcaaacccaa
     1261 gggcaagctt agcacagcaa cgaagtgggt cagatggata gcgtacacta gatagtaact
     1321 atacgtgaag cgctatccac tcgctcgtaa acaccaggca cccgtattaa atcatttcca
     1381 accggtcaaa ccaaaagaat aagcgagatt gaatattgaa cgtgaggtga agtccgccac
     1441 tttaccctaa ttttcacgtt acgcaacacc aaaaacagca gttgagcgat caaaggttgg
     1501 ggatacaggg tttaggggtt ggggatacaa cctgtttttt cctctgagag atctcgctga
     1561 tctcacgtcc tgaatccatc tgagcaacct gtgattccct cgcacaaggt tttacttctg
     1621 ttgattggtc agcagcaggt tcccttttgc atcccgccca acccgcaaag gtgccgaacg
     1681 aggggcggcg gccaatccgc cagtttcagc taaaccattg taaatattac ctgccacgat
     1741 agaagcgagt gagccacgct ggcgtgaacc ggtggtaatc accgaaatga caaggcttgg
     1801 gtcaaccggg ttgataaaag aagtaaacag ccccaatttt gttccctgag cacaatagca
     1861 atcacaactc ccggtctttc cggctacggc cagactggca gcatttgacc ggcgagcggt
     1921 gccgtaatca acggcagcca gcattccttc gatcattttt gcccgtgcag cctcaggcaa
     1981 agaaatggtt ccggcaactt caggtttaaa atgtttgata tcgtcctgag tgcgcaaaat
     2041 ctgtggttta tagaccgtgc ctccattcgc aatggcggca gtaaacacgg ccagttgaac
     2101 ggcagtgatc ccaaatccat caccatgact aaatattcga cgcaggttat tggctggggt
     2161 tggcaaatag ccggtgcttt cacctggaac attgacttca gtcggacggc caaacccaac
     2221 ttcacgggca taggccagca tccggtctaa cccaatctgc tcgccgagat cctgaaagta
     2281 ttcattgttt gagcgcgcca gggcctgggt catcgtaaac tgggttcgtc caaacgccag
     2341 cggggtatct ggatcagcaa tgccttctcg taaggcggcg agggaggtca acaatttaat
     2401 ggtggaacag ggtttaaacg g
//
LOCUS       DAOQDO010000881         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_38983_length_2420_cov_3.213953, whole genome shotgun sequence.
ACCESSION   DAOQDO010000881 DAOQDO010000000
VERSION     DAOQDO010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38983_length_2420_cov_3.213953"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKZ53_13935"
     CDS             <1..279
                     /locus_tag="PKZ53_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41588.1"
                     /translation="TKNQAPRTKHQEPRTKNQEPRTRRNGLSKHTSAHFRGFNFPLHF
                     PGQFLFISSNSLYFSYFTTGENWIAVNGCRGLPADAFCVWAVANSDNG"
     gene            236..2296
                     /locus_tag="PKZ53_13940"
     CDS             236..2296
                     /locus_tag="PKZ53_13940"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ41589.1"
                     /translation="MPSVYGQSPIQITADRFGGGNVVDLQSVPWRYHPGDQPEWAQIE
                     CDDGHWELISNTHFLSDHLPSSGWQGSGWFRLTVNVEPDVVHLPLNVTLQQLGASEVY
                     LNGKLIQRFGQVGTSSISEQPFNPGLVPFGFVFNRSGPNLIAVRLSNQAMADMRSGWG
                     RWLSRTGYGAGFRMQLRDAGQSQIEQEQDFTWNASLSLGTAGVFAAFGILHLLLFVFY
                     PKQRANLYFSLYALGVAWIVLAFFWIATGHQGAIGTAGLFFTVWFGIDLTNVAILAFL
                     YAAVSQPIPRYFWWVLTAWGVLLLSGIPFPNSQAGLWLYTGLSFFTAVESLRIVGKAL
                     IQKLDGGWIIAIAMVLVALNPLKEALVNAGLLEVPPMVDRVHGLTFGVIVSISIYLAR
                     TFAQTNQSLEQQLEQVKVLSEQQLESERQQAELRIQHEQEKARLAKIEAENERRAQEL
                     EEARQLQLLMLPKSVPQLKNLEIAVFMRPATEVGGDYYDFQVSADGTLTVAVGDATGH
                     GLKAGTMVTAAKSLFTNLAHEEDLEYIFKQSSRVLKEMNLRSLYMAMGMLKIKGWQAT
                     VCLAGMPPILHYRADSQTVEEILVEGMPLGSPVRFPYQQQELTLGPGDVVVLMSDGFP
                     ERFNAEDDIFGYPKVEEVFAALVHQSPDEIIRQLVKAGDDWAGDHPQDDDITFVVLKM
                     KAAG"
BASE COUNT          566 a          578 c          682 g          594 t
ORIGIN      
        1 accaagaacc aagcaccaag aaccaagcac caagaaccaa gaaccaagaa ccaagaacca
       61 agaaccaggc gcaatggatt gtcaaaacat acatcggccc attttcgggg cttcaatttt
      121 ccgcttcatt ttcccggtca atttcttttt atttcatcga actccttata cttcagctac
      181 ttcacaactg gtgagaactg gattgctgtt aatggttgcc gcggtctgcc tgccgatgcc
      241 ttctgtgtat gggcagtcgc caattcagat aacggctgac cggtttggtg gtgggaacgt
      301 ggttgatttg caatctgtcc cctggcgcta ccatccaggt gaccagccgg aatgggcaca
      361 gatcgagtgt gatgatggtc actgggaact catatcaaac acacactttc tgtctgatca
      421 tctcccctca agtggctggc agggaagtgg ctggtttcgg ttgacggtta acgttgagcc
      481 agacgttgtg caccttccat tgaacgtgac gctgcagcaa ctgggtgcct cggaagtgta
      541 cctcaacgga aagctgatcc agcggtttgg acaggtcgga acatcatcca tttctgaaca
      601 accgtttaat ccggggctgg ttcctttcgg gtttgtattt aatcggtcag gtcccaattt
      661 gattgcggtt cgactttcca atcaggccat ggctgatatg cgttccggat ggggacggtg
      721 gctttcgcga accgggtatg gcgccggatt tcgaatgcaa ttgagagatg ctggccagtc
      781 ccaaattgag caggagcaag attttacctg gaatgcaagt ctcagtctgg gaaccgccgg
      841 agtgtttgcc gcatttggga tcctccattt gctgctcttt gtattttatc caaaacagcg
      901 ggccaatttg tatttcagcc tgtatgccct gggtgtggcc tggattgtgc tggctttttt
      961 ctggattgcg actggtcatc agggcgccat tggcaccgcc ggtctgtttt ttacggtgtg
     1021 gtttgggatt gacctgacca atgtggccat tctggcattt ctctatgcgg cggtttcaca
     1081 accgattcca cgctatttct ggtgggtact gactgcctgg ggtgtcctgc ttctgtcagg
     1141 aattccattt ccaaactcgc aagccgggtt gtggctatac acagggctct cgtttttcac
     1201 cgctgttgaa tcgctgcgaa tcgttggcaa agccctgatt caaaagctag atggcggctg
     1261 gattattgcg attgcaatgg ttctggtcgc gctcaacccg cttaaagaag ccctggtcaa
     1321 tgccgggctc ctggaagttc caccgatggt ggaccgggtc cacgggttga cgttcggagt
     1381 gattgtttcg atttcaattt atctggcccg cacctttgcc caaaccaacc agagcctcga
     1441 acaacagttg gaacaggtga aagtgctttc agaacaacag cttgaaagcg aacgacagca
     1501 ggccgaattg cgaatccaac acgaacagga aaaagcccga ttggccaaaa tcgaagctga
     1561 aaacgaacgc cgcgcccagg aacttgaaga agcccgtcag ttacaactgt tgatgctgcc
     1621 taaatcagta ccgcaactca aaaacctgga aattgcggta tttatgcggc cagccactga
     1681 agttggcgga gattattatg attttcaagt gtcggcggac ggcacattga ccgtggcggt
     1741 gggagacgcc actggtcacg gcctgaaagc tggaaccatg gtcacggcgg ccaagagttt
     1801 gtttaccaat ctggctcacg aagaagacct ggagtacatc ttcaaacaat catcacgggt
     1861 gctcaaagaa atgaacctgc ggtcgctcta tatggccatg ggcatgctga aaatcaaagg
     1921 ctggcaggcc acagtgtgtc tggcgggcat gcctccgatc ctgcactatc gcgctgattc
     1981 tcaaaccgtt gaggagattt tggttgaagg catgccactg gggagcccgg tcagattccc
     2041 ttatcagcaa caggaactga cacttggacc aggggatgtg gtggtcttga tgagcgatgg
     2101 atttcccgaa cgctttaatg cagaagatga catttttggc tatccgaagg tcgaagaagt
     2161 gttcgctgcc ctggtgcatc agtcaccgga tgaaatcatt cgacaactgg tcaaagctgg
     2221 agatgattgg gccggagatc atccccagga tgacgatatt acctttgtgg tgttgaagat
     2281 gaaggcagcg ggctgaagaa cgtcgggctg ggggctgaaa acatcgggct gggggctgaa
     2341 ggaaacgggc ttgggggctg aaaacatcgg gctgggggct gaagaaaacg ggctgggggc
     2401 tgaagaaaac gggcttgggg
//
LOCUS       DAOQDO010000882         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39065_length_2418_cov_2.298773, whole genome shotgun sequence.
ACCESSION   DAOQDO010000882 DAOQDO010000000
VERSION     DAOQDO010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39065_length_2418_cov_2.298773"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /locus_tag="PKZ53_13945"
     CDS             complement(<1..2132)
                     /locus_tag="PKZ53_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ41590.1"
                     /translation="MPNPVPPIRTTLTSFLADFLNRGDETAFAVKRGVRVIRWSYRRT
                     AETAFQIARELTTQHTKPGDRILIWAENGPEWVAAFFGCLLVGGVAVPLDPQSTPEFI
                     RQIIRHTGAKLGFCDSTTHPQVPSEISWLRLETIQDAVSKHPPVPWHPVEIKPTDVAE
                     IIFTSGTTAEPRGICLSHRNLLVNLQPLEQEIHKYLKWERPFHPIRFLDLLPLSHVFG
                     QFMGVFVPQLLGGEVHFQSSVNPTEILETIRRQRISVCVIVPRLLETLRQKVERDWLA
                     SHPGQSLQKMLNECPKEHFARRWWRFRQVHNQFGWKFWAFISGGATLDPNLEAFWRHL
                     GFALVQGYGMTETASLVSVVHPFKLSQGSIGKALPGRELRLDEGGEILVRGENIAAGV
                     WQRGNIEPLSDDGWLRTGDLGFRDSEGNLFFRGRKKEVIVTGAGMNIYPEDLETALLR
                     QPEIRQATVFGIEGQTGPEPVAVVIVGDEKTAVDQVLATVNAGLNPYQHIRRWLVWPG
                     PDFPRTSTHKIRKRDVIAWAAAQLGAGQSQPPGDEFTSDFLFTAIEKITREKPVRFDP
                     EASLTTDCRLDSLGKVELLSWLEDHFHIELDEAAFTAATTVGQLQHLIEQVIGATSRP
                     GAPELKASPVEPQPVSDAPYPFPAWAMRAPWTWLRQLLFYSLVWPVCAVLCWTRVEGR
                     DHLAHLTGPVVLVANHITIADHALILAVL"
BASE COUNT          564 a          679 c          647 g          528 t
ORIGIN      
        1 agtaccgcta aaatcaaggc atggtcggca atggttatat gattggccac cagcaccacc
       61 gggccagtca ggtgagccag atggtcgcgg ccttccactc gcgtccaaca caacacggca
      121 cacaccggcc agacaaggct gtaaaacagc agttgccgca gccacgtcca cggagcacgc
      181 atcgcccagg ccgggaatgg atagggtgca tcagaaactg gctggggctc aacgggcgac
      241 gcttttaact caggcgcacc agggcggctg gttgctccaa tcacctgctc aatcaggtgc
      301 tgaagctggc ccactgtggt tgccgctgta aatgcggctt catccaattc gatatgaaaa
      361 tggtcttcaa gccagctcaa gagttcaacc tttccaagcg aatcaagccg gcaatcggtt
      421 gtcaaactgg cttctgggtc aaatcggact ggtttttcgc gggtaatttt ttcaatcgcc
      481 gtgaacagaa aatctgacgt gaactcatcg ccaggcggtt gactttggcc agccccaagc
      541 tgtgcggcgg cccaggcgat gacatcgcgc ttgcggattt tatgggtgct ggtccgagga
      601 aaatcaggcc ccggccacac cagccatcgt cgaatgtgct ggtacgggtt taatccagcg
      661 ttcacggtgg ccaggacttg atcaaccgct gttttttcat cccctacaat caccacggcg
      721 accggttccg gtccggtttg accctctatc ccaaacacgg tggcctgccg gatttcaggc
      781 tgccggagca gagcggtttc caaatcctcg gggtagatat tcattccggc acccgtcaca
      841 atgacctctt tttttcgtcc ccgaaaaaac aggttgcctt ccgaatctcg aaacccaagg
      901 tcacccgttc gcagccatcc atcgtcgctc agtggttcaa tattcccacg ttgccagacg
      961 cccgccgcaa tattttcacc ccggaccagg atttccccgc cctcatcaag cctgagttcc
     1021 cgacctggaa gcgcttttcc gattgagccc tggctgagtt tgaacggatg caccacgctc
     1081 accagcgaag ccgtttcggt cattccatag ccctggacta aggcaaatcc caggtgccgc
     1141 cagaaggctt cgaggtttgg atccagcgtg gcaccgcctg aaataaaggc ccagaacttc
     1201 cagccaaact ggttgtgaac ctgccgaaac cgccaccagc gacgggcaaa atgttccttt
     1261 ggacactcat tcagcatttt ctggagtgat tgaccaggat gcgaggccag ccagtctcgc
     1321 tctacttttt gccgcagggt ttcaagcagc ctgggaacaa tcacgcagac ggaaatccgc
     1381 tgccggcgaa tggtctccag aatttctgtt ggattgacgc tggattggaa atggacttca
     1441 ccaccgagca actgaggcac aaaaacgccc ataaactgcc caaacacgtg acttaacgga
     1501 agtaaatcca ggaatcgaat cggatgaaaa ggccgctccc attttaaata cttatggatt
     1561 tcctgttcga ggggctgcag attgaccagc agattgcggt gtgacaggca aatacccctg
     1621 ggttccgccg tcgtgcctga agtgaaaatg atttcagcca catcagttgg cttgatttca
     1681 accggatgcc agggcaccgg cggatgtttt gaaactgcat cctggatggt ttccaaccga
     1741 agccatgaaa tttctgaggg aacctgggga tgcgtggtcg agtcacaaaa gccaagtttg
     1801 gctccggtgt gccggatgat ttgcctgata aactcaggtg tgctctgggg atcaagcggt
     1861 accgcaacac cgccaaccag caaacatccg aaaaaagctg caacccactc agggccattc
     1921 tcagcccaaa tcagaatccg atctccaggc ttcgtgtgct gagtggtgag ttcgcgtgca
     1981 atttgaaacg cggtctcagc cgtccgccga tatgaccagc gaatgacccg cacgcctcgc
     2041 ttcaccgcaa acgccgtttc gtcgccacga ttgagaaaat cagccagaaa gctggtcagt
     2101 gtggttcgga ttggtggcac ggggttgggc ataacgggtt cctgattcca agttcttgga
     2161 agggatgagg ctgcggcctg gaattgccca aaagacggta atcatcaccc agtgctcgtg
     2221 gacgattgtt ccgactcaca ccgtgacatt caggacaggc gtgtgaggtg tccgccacct
     2281 ggtggttcgt tgtaccgcgc caggttggag cgcttccttg accgaaagcc gcggggtatc
     2341 cgcttcggtc acaactgggc aatcaagttt gaggtgatgg ttttcaactc gaatgagtga
     2401 ggaagtttaa attacgaa
//
LOCUS       DAOQDO010000883         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39082_length_2417_cov_3.641829, whole genome shotgun sequence.
ACCESSION   DAOQDO010000883 DAOQDO010000000
VERSION     DAOQDO010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39082_length_2417_cov_3.641829"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..>2417
                     /locus_tag="PKZ53_13950"
     CDS             206..>2417
                     /locus_tag="PKZ53_13950"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HNJ41591.1"
                     /translation="MTFRRTVWSLVLVVGGILGCSQSVLAQAGVVIPGDSSTPDVKQL
                     SLAEMSTDVRIDHQYARVKIVQIYANHTSQVLESRYIFQLPTTGAISDFAVWDGDIRI
                     PGVILEVKRATEIYEELKAQYIDPGLVTQEDEEGGSTAFTVRLTPIPAYGTKRVEMEY
                     TQLLPVEGSQTFFSLPLKPSEYGQQMAGLFSLSLDVMSRIPMSQLNVRSTQYPLAFSV
                     QQPDHIVGAFTGTNVSFGQDFAFDYTLQNTKTELSLLTYRAPEIISPLELRDPNRIKP
                     DNDGYFEIAAVFNERGAKPGEFIKSTTEKRSVVILLDTSLSMQWEKLDRAFQATELFL
                     RTLAAQDSFNVLLFNDDVQVLSSTPLTATPENIERALTFIKQSYLSGGTDFVGALKKG
                     LELAKQLPGDERDLILITDGNPTLTTTLSRRMVQDFSTANGSGTKRIARVYAFGIGSD
                     TNQQFLKELARTSRGYFDWGRETDELEFRLTAFFAKVGQKPIEALALTSSDAANIYSV
                     YPDVDTTSYDGSRFSFVGRYRRPAQSITFTLAGDQHGRPIKLAASTALPELDNTHSHL
                     PRMWARQRVDALLRLIALNGEDEALIAEIIALSKKYKFVTPYTAFLAAPRSLLRPRVI
                     KPGDPILRVRTDASIKSVVAVFPFGLTKPLTYLTAEDIWETRFLAPKEMQDGSYACRL
                     ILTDDKGNVFQEEKRFVIDSRPPQLKPTVTKSLAQAGDSIEIQVAADQDTRRISA"
BASE COUNT          577 a          647 c          605 g          588 t
ORIGIN      
        1 cccaggtttg atctactgac gactgacgac tgacgactga cttgaattgc tacttcgcta
       61 ttttccccat cagtggtaat ctgtgcaggc caacacgtca gattcttgta atttttttcc
      121 agtttgttca ccttcaaata cagaagcctc aacgcacaag tcagtcagga acccaggaca
      181 cattatgttt tgttgttcat ttgctatgac ttttcgaaga acggtgtggt cgttggtgct
      241 ggtcgtgggc ggcatactcg gctgttccca atcagttctc gcccaggccg gagtggtcat
      301 cccaggagat tcctcaaccc cagatgtaaa gcagctttca ctggccgaga tgtcaaccga
      361 tgttcggatt gaccatcagt atgcgcgggt caagatcgtt cagatctatg ccaaccatac
      421 cagtcaggtg cttgaatcca ggtatatttt tcaattgccg acaaccggag ccatttcgga
      481 ttttgcggtg tgggacggag atattcgcat tcctggcgtg attctggaag tcaaacgggc
      541 aactgagatc tatgaagaac tcaaagccca gtacattgac cccggcctgg tcacacagga
      601 agacgaagaa ggcggcagca cagcctttac cgtacgcctg acgccgattc cagcttatgg
      661 aaccaagcga gttgagatgg aatataccca gcttttgccg gtcgaaggca gtcagacatt
      721 tttctcgttg ccgctcaagc catcggaata tggccagcaa atggcgggtc ttttcagcct
      781 gtcgctggat gtgatgagtc ggattccaat gtcccaactc aatgtgcgaa gcacccagta
      841 cccgctggcc ttttcggttc agcaacccga ccacatcgta ggggctttta ctggcaccaa
      901 tgtttcgttt ggccaggatt ttgcctttga ctacacgctg caaaatacca aaaccgaatt
      961 gagcctgctg acttatcgcg ctccggaaat tatctcaccg ctggaattac gtgatccaaa
     1021 ccggatcaaa ccggacaacg atgggtattt tgaaatcgcc gccgtcttta atgagcgagg
     1081 ggccaagccc ggtgaattta tcaaatccac caccgaaaaa cgctcggtcg tgatcctgct
     1141 tgatacgtcg ctttcgatgc agtgggagaa actggatcgg gcgtttcaag ccaccgagct
     1201 atttttacgg acactggctg cccaggatag ttttaacgtg ctgctcttta atgacgacgt
     1261 gcaggttttg tcctccactc cactgaccgc gacgcctgaa aacatcgagc gagcactgac
     1321 atttatcaag caaagctatt tgtcaggtgg gacagatttt gtcggtgcgc tcaaaaaagg
     1381 actggaactg gcgaaacagt tgcccggtga cgagcgcgat ttgattttga ttactgatgg
     1441 aaacccaacc ctgaccacca cgctctcacg tcggatggtt caggatttct ccaccgccaa
     1501 cggaagcggc accaaacgaa tcgcccgggt gtatgctttt ggaattggct ctgacaccaa
     1561 tcagcaattt ctgaaagaac tggcacgcac cagccgtggc tattttgact ggggacgcga
     1621 aaccgacgag ttggaattcc gattgacggc cttttttgcc aaggttggcc aaaagccgat
     1681 tgaggcgctg gccctgacct ccagtgatgc ggccaatatc tattcggtct atcccgatgt
     1741 ggatacaacc agttatgacg ggtcacggtt ttcatttgtg ggacgctatc gtcgtccggc
     1801 gcaatccatc acgtttaccc tggctggcga tcagcatggc cgcccgatca agcttgccgc
     1861 cagcactgcc ttgccggaac tggataacac ccacagtcac ctgccacgaa tgtgggcacg
     1921 ccagcgcgtt gacgccctgc tccggttgat tgctctcaat ggcgaagacg aagccctgat
     1981 tgccgaaatc atcgcgctct ccaaaaaata caaatttgtg acaccgtaca cggcctttct
     2041 ggcggctccc cggtcattgc tcaggccgcg tgtcatcaag ccgggtgatc cgattctcag
     2101 ggttcgaact gatgcttcaa tcaagtctgt ggtggcagtg tttccatttg gcttgaccaa
     2161 gcccttaacc tatctcacgg ctgaagatat ctgggaaacc cgatttttag ctccaaaaga
     2221 gatgcaggat ggcagttacg cctgtcggtt gatcttgacc gatgacaaag gaaatgtatt
     2281 tcaagaggaa aaacggtttg tgattgacag ccgtccacca caactcaagc cgacagtaac
     2341 caaatcactg gcacaggctg gtgactcaat cgaaatccag gtggccgccg atcaggatac
     2401 tcgccgcatc tcagccc
//
LOCUS       DAOQDO010000884         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39215_length_2413_cov_2.380831, whole genome shotgun sequence.
ACCESSION   DAOQDO010000884 DAOQDO010000000
VERSION     DAOQDO010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39215_length_2413_cov_2.380831"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..956
                     /locus_tag="PKZ53_13955"
     CDS             147..956
                     /locus_tag="PKZ53_13955"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNJ41592.1"
                     /translation="MADDTLTPESPTPSGSEIRWRVSGASVCGSGHQRHHQPCQDACQ
                     WQVVNSRWLVAAVADGAGSATHAEIGARLVVETVVAHLTAQISANQFQIEADEVLQTS
                     LTNAFQLARTKLEQEAETHGIALRELATTLVVVVAHPEVVAVAQIGDGAAVVKPQRLD
                     PLALTTPHTGEYLNQTLFLTAPQALTQIQTVVWRGSLAQLALFTDGLQLLALKLPEAT
                     AHRPFFAPLFQFMAEETDPASATEQLVGFLNSNRIRERTDDDLTLVLATIE"
     gene            1288..>2413
                     /locus_tag="PKZ53_13960"
     CDS             1288..>2413
                     /locus_tag="PKZ53_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41593.1"
                     /translation="MRLQRKKSGDLVFLNDSQVLGTGGEGKVYAVPQDESLVAKIYHS
                     AADVPASKLLAMLDYSPVDPTREEHHASIAWPVDLLVDPAHPGTVLGYVMPRVEKMHP
                     IFAFYNPASRLTTSPLFTYTYLYRTARNLMAAVTALHEAGCVIGDVNESNILVSETAL
                     VTLVDTDSFQIRNLQTAEVYRCPVGKPQFTSPELQGVRFSEVDRTPEHDVFGLAVILF
                     QLLMEGTHPFDGVYQGAGDPPLQEERIAAGHFPYSSGPRIPYTPKPIAPPFEILPPAI
                     QSLFRQCFEAGHQDPQKRPSARLWRDALYDAEKNLIGCTARPLHTYSPHLPACPWCER
                     TEKLGGRDPFPTLEQAQERAALRKARARSTTGQTGSAGPYA"
BASE COUNT          546 a          659 c          638 g          570 t
ORIGIN      
        1 ggctggtcgt gcgcacgccg ctcaagctca aaggattgaa ttttaaggac atgttcacct
       61 ggctttcagt cagtatgcag cgggtgtcac actccaggct tgacgaacag gtggcgctgc
      121 caccgccagg atgggggaca gtttaaatgg cggatgacac tttgacgcca gaaagcccaa
      181 ctccatcagg ctcagagatt cgatggcggg tcagcggcgc ttcggtttgt ggttccggcc
      241 accagcgcca ccatcaaccc tgtcaggatg cctgtcagtg gcaggtggtc aattccaggt
      301 ggctggtggc ggcagtcgct gacggcgcgg gttcggccac acacgctgaa atcggtgctc
      361 gtctggtggt tgaaacagtt gtggctcatc tgactgccca aatcagcgcg aaccagtttc
      421 agattgaagc tgacgaagtg ctccagacct ccttaaccaa tgcctttcaa ctggcacgaa
      481 caaagctgga acaggaagcg gagacgcatg ggattgcgct tcgcgaactt gccacgacgc
      541 tggtggtggt ggtggctcat ccagaagtgg ttgccgttgc tcaaattgga gacggtgccg
      601 cggttgtaaa accccaacgt cttgaccccc ttgccctgac aaccccacac accggagagt
      661 acctcaacca gacgttgttt ctcaccgctc ctcaggccct gactcaaatt cagacggttg
      721 tctggcgcgg cagcctggca caactggcgt tatttaccga tggcctccag ttgcttgccc
      781 tcaagttgcc cgaagccacg gctcatcgtc cgttttttgc ccctttgttc cagtttatgg
      841 ctgaagaaac tgacccggcg tctgccacag agcaactcgt cggtttctta aattcgaacc
      901 gcatccgcga acggaccgac gatgacctga cactggtgct ggcaaccatt gaatgaagaa
      961 tgaagaattg agaatgaaga attccatttg ggaatggagt ggttgtatta ggttctgttg
     1021 acatttcttt gccgcacagt agaactgtga attgagaccg gggcttcagc gccggtaatg
     1081 accaaaatcc aggatcaaag gcggtcaaat cgtgatttgt ccagtttccc ggccttgaaa
     1141 ggccagtctt aactcacagc tccactatgc ggtttaatat acgcctcaaa atgtcaacag
     1201 aacctagaaa acggtcaaat aattcaatca aatgggctta gtgatctact gattactgac
     1261 cactgactat tcccataatc cactgaaatg cgccttcaac gaaaaaaaag cggggatttg
     1321 gttttcctga acgattcaca ggtgctgggc actggcggtg aagggaaggt ctatgccgtt
     1381 ccgcaggatg aatccctggt tgcgaaaatt taccattcag cggctgacgt tccagcgtca
     1441 aagttgctgg ccatgttgga ttattcccca gttgatccaa cccgtgagga gcatcacgcc
     1501 tcgattgcct ggccggttga cctgctggtt gatccggccc atccgggaac tgttctcggg
     1561 tatgtcatgc ccagggttga gaaaatgcac ccgatttttg ctttttacaa cccggccagc
     1621 cggctgacca ccagcccctt gtttacttac acctatttgt accggacagc tcgtaacctg
     1681 atggcggctg ttactgcgct acacgaggct gggtgtgtca tcggcgatgt caatgaatca
     1741 aacattctgg tctcagaaac ggcgctcgtg acgctggttg ataccgattc gtttcagatt
     1801 cgaaacctcc aaactgcaga agtgtatcgc tgtccggttg gaaaaccaca gtttacgtca
     1861 cccgaactcc agggcgttcg gttttctgaa gttgatcgaa cccccgaaca tgatgtgttc
     1921 ggcctggccg taattttgtt tcagctcttg atggaaggta cgcatccgtt tgatggtgtg
     1981 tatcagggcg cgggtgatcc tccgctccag gaagaaagaa ttgctgccgg gcattttccc
     2041 tatagcagtg gaccgcgaat tccatacacg ccgaagccaa ttgccccacc gtttgagatt
     2101 ttgcctccag ctattcaatc cctgtttcgc cagtgttttg aagccgggca ccaggaccct
     2161 caaaaacgac cttcggcccg gctctggcgt gatgcactct atgacgcaga gaaaaatctc
     2221 attgggtgta ctgcccgacc gctgcatacc tatagcccac atctgccagc ctgtccctgg
     2281 tgtgaacgca ctgaaaagct tggcggacgc gacccgtttc cgactctgga acaggcccag
     2341 gaacgagctg cgttacgcaa agccagagcc cgttcaacca cgggtcagac ggggtcagcc
     2401 ggaccttatg cct
//
LOCUS       DAOQDO010000885         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39227_length_2412_cov_4.022062, whole genome shotgun sequence.
ACCESSION   DAOQDO010000885 DAOQDO010000000
VERSION     DAOQDO010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39227_length_2412_cov_4.022062"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="tmk"
                     /locus_tag="PKZ53_13965"
     CDS             <1..525
                     /gene="tmk"
                     /locus_tag="PKZ53_13965"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNJ41594.1"
                     /translation="THSPAESGAISSSAIPQLPPTPLAEALLYAADRAQHVETVVRPA
                     LLAGRVVLCDRYIDSTLAYQGIGRQRDLALIQNLVTIATQGLKPALTLLFDLPVAESQ
                     VRLHGSAAHRPAGQLDRLDSEPLDFHERVRTGYLSLAAAEPDRFRVLDACCDIETLHQ
                     RVVACLPPELLNRG"
     gene            677..1612
                     /locus_tag="PKZ53_13970"
     CDS             677..1612
                     /locus_tag="PKZ53_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015191365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNJ41595.1"
                     /translation="MPDLSSPFVLIVPTGTQIVTRIACRALDSAHYYPPGAVGVIVNT
                     PVDHTHSYRIRFPDGQESTLLRHQFSLRKQFQSQGLTSNEASLGETDWSDYIIFRCVV
                     GSRAYGLDHAGSDTDRRGIYVPPADAQWSLFGVPEQLESKETEECYWEIQKFLILALK
                     ANPNVLECLYSPLIETATPLAEELLGMRSIFLSRLVYQTYNGYVMSQFKKLEQDLRSR
                     GSIKWKHAMHLIRLLLSGITTLREGFVPVQVAAFREQLLAIRNGELDWAEINAWRLSL
                     HQEFDRAFEASSLPERPDYEKANAFLIHVRRSQLL"
     gene            1634..>2412
                     /locus_tag="PKZ53_13975"
     CDS             1634..>2412
                     /locus_tag="PKZ53_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNJ41596.1"
                     /translation="MHQIAPDHLAQLQSILVIQPFPFLFVTVSGAHLYGFASPDSDFD
                     IRGTHILPLTEALRLEPKNETLELSTVYPNGLELDLVTHDVRKFFGMMLKRNGYVLEQ
                     LFSPLILQTTPEHDELKRIARHCLTRHHSHHYLGFAATQWKLFEKETPLRVKPLLYVY
                     RVLLTGIHLMETGEVEANLVHLNEVFGLPYLAELIARKQAGPEKGTLNDADVEFHRRE
                     FNRLVQKLEATQQTTHLPDRPGPTAQAELDDLLVQVRLQSQT"
BASE COUNT          547 a          638 c          600 g          627 t
ORIGIN      
        1 actcactcac cggcggaatc tggcgcgatt tcctcttctg ccattcctca acttccgcca
       61 acaccacttg ctgaggcact cctctatgcc gctgaccggg cccagcacgt cgaaacagtt
      121 gtgcgtcctg cgcttctggc aggtcgtgtt gtcttgtgtg atcgctatat tgactccaca
      181 ctggcgtatc agggaattgg ccgacaacgt gatctggcgt tgattcaaaa tttagttacc
      241 attgcaactc agggattaaa gccagcttta accttgttgt ttgatttgcc ggtggcagaa
      301 agtcaggtac gcctccacgg ctcagccgcc caccgtccag caggtcagtt agatcgcctt
      361 gactcagagc cacttgattt tcacgaacgt gtccggactg gttatctctc gctggctgcg
      421 gctgaaccag accggtttcg ggtgctggac gcctgttgtg acattgaaac attgcatcaa
      481 cgggttgtgg cctgtttgcc tcccgagtta ttgaatcggg gctgaaaaaa ccagggtcag
      541 gagctgaaga aagcgggctg agggctgaag actcgcaagc tcggggctga agaccagggg
      601 gaaaaaccac tcagcccgag ttcttcaacc ccaaattctt caacccgatg tcttcagccc
      661 gaaatgcttc agtttcatgc ctgatctttc ttccccattt gttttgattg ttccgactgg
      721 cactcaaatt gtgacccgga ttgcctgtcg agccctggat tcggcccatt actacccacc
      781 tggggccgtt ggtgtcattg tgaatacccc ggttgatcac actcattcct accggattcg
      841 atttccagac ggtcaggaaa gtacgctgct gcgccatcag ttttctctgc gtaaacaatt
      901 tcagagccag gggttaacct caaatgaggc cagtttgggc gagaccgact ggtcagacta
      961 tattattttc cgatgtgtcg ttggctcgcg cgcttatggc ctcgaccacg ctggttccga
     1021 tactgaccgc cggggaatct atgttcctcc agccgatgca caatggtcac tgtttggtgt
     1081 tccggaacaa ctggaaagta aagaaacaga agagtgttac tgggaaatcc agaagttttt
     1141 aatcctggcg ctcaaagcca atccaaatgt cctggaatgc ctttattctc cgttgattga
     1201 aactgccacc cctctggctg aagaactgct cggcatgcgc tcgatttttc tttcccgtct
     1261 ggtgtatcaa acctataacg gatatgtcat gtcccaattt aagaagctgg aacaggatct
     1321 tcgctcacgg gggagcatca aatggaaaca tgccatgcac ctgattcggc tgctgctttc
     1381 agggatcacg acgttgcgcg aagggtttgt tccggtacag gttgctgctt ttcgagagca
     1441 attgctggct attcgcaatg gggaacttga ctgggcggaa atcaatgcct ggcggttaag
     1501 cctccatcag gaatttgatc gggcatttga ggcatcatct ttgcctgaac gccctgatta
     1561 tgaaaaagca aacgcatttt tgattcatgt ccgacgaagt caacttttgt gaggctgaca
     1621 gacaaggaac cttatgcatc aaatcgcccc tgaccatctg gctcaacttc aatctatcct
     1681 cgtcattcag ccttttccat ttctgtttgt gacggtgagt ggcgcccacc tgtatggatt
     1741 tgcttcgcct gattcggatt ttgacatccg agggacgcat atcctgccac ttactgaggc
     1801 cttgcggtta gagccaaaaa acgaaactct tgaactatcc accgtctatc caaacggatt
     1861 agaactggat ctggtgaccc atgacgttcg gaagttcttt ggcatgatgt tgaaacgcaa
     1921 tggctatgtt ctggagcaac tgttttctcc cctgatcttg caaaccacac ccgagcatga
     1981 cgaactcaaa cgcattgctc gccactgtct cacccgtcac cacagccatc actatttagg
     2041 gtttgccgcc acacagtgga agttgtttga gaaggaaacc ccgttacggg tgaagcccct
     2101 gttgtatgtg tatcgggtgc tattaaccgg aattcatctg atggaaacgg gtgaagtcga
     2161 ggccaatttg gtgcacttaa atgaagtatt cgggttgccg tatctggctg agttgattgc
     2221 ccgcaagcaa gccggcccgg aaaagggaac tctgaacgat gccgacgtgg agttccaccg
     2281 gcgtgaattc aatcgcctgg ttcagaaact ggaagccact caacagacca cgcatcttcc
     2341 tgatcgtcca ggccccacag cccaggccga gctggatgac ttactggttc aggtgcgtct
     2401 tcaatcccaa ac
//
LOCUS       DAOQDO010000886         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39268_length_2411_cov_2.798387, whole genome shotgun sequence.
ACCESSION   DAOQDO010000886 DAOQDO010000000
VERSION     DAOQDO010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39268_length_2411_cov_2.798387"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..902)
                     /gene="ruvA"
                     /locus_tag="PKZ53_13980"
     CDS             complement(273..902)
                     /gene="ruvA"
                     /locus_tag="PKZ53_13980"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNJ41597.1"
                     /translation="MIAFLTGKLLEKNPSNFIVDVGGIGYDVFIPLSTFYQAGENGSP
                     ISLHIQMIVREDALILYGFSTKLEKEIFIRLISVSGVGPKLALTILSGLTIDELISAL
                     KNGDIRRLSGIPGIGKKTAERLVVELRDKLTALSVPETGGNSAPASTPESPPGQHQET
                     LLKSDLVSALVNLGWQTAAAEKAVTQCLKEESRREFSFLLKESMKRLYR"
     gene            1148..>2411
                     /locus_tag="PKZ53_13985"
     CDS             1148..>2411
                     /locus_tag="PKZ53_13985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ41598.1"
                     /translation="MKLLVNTILDVTVNPADWTSRVEIPCCLGKDGEDFKDQSQSDST
                     HKKMKLMKRILIVDDDIEFRQVLEQLMLHEGHWVRVAESSAKAIQLFREMANTPQPFD
                     LVISDMMLGDGSGLDVLSVVREVTTDMPFIFVTAFGSMESVAEAIRKDVFDYVAKPFD
                     AGELLRVVRRALSKLSTEIEPTLEVPDSVAGSLKIIGSSPPMIELYKAVARVSATDST
                     VLMSGESGTGKELVARAIHNNSLRAHRPFIGVNCGAFPETLLESELFGHAKGSFTGAN
                     TERIGIFEAAAGGTVFLDEISETSPNFQVKLLRVLQERELDRVGGEQPVKIDVRVISA
                     TNKKLGDEVAAGRFREDLFYRLQVVPIVLPALRERLEDLPLLVQHFIATLGPRTNPKV
                     RTIDEAALERLSAHHWPGNIRELGNVIEQ"
BASE COUNT          612 a          542 c          641 g          616 t
ORIGIN      
        1 ggcagggaag gtgtgaggtt cttcgaaggc tcccccgacc agaatcaggc aaattcaaat
       61 gccagatctt tgttcaggtt tttttggagg tggcctcagc aggtgtagtt gacaggttca
      121 gactttgggg atttcgaata aacgggtgac cctctgcgga gtggatcgtt ggaatcttca
      181 tctgccacca gtggtttggg tcataaggtc accgtcagga gacccaaaga cgaaagcgtt
      241 caaacctgga caccctgagc aagagggagg ctctagcggt agaggcgttt cattgactct
      301 tttaagagga aagagaactc acgtcgtgac tcttctttga ggcattgggt gactgctttt
      361 tcagccgctg cagtttgcca cccgagattg accagggctg acaccaggtc tgatttgagg
      421 agtgtttcct gatgttgacc ggggggtgat tccggggttg aagctggtgc tgagtttcca
      481 cctgtctctg gtacggagag tgcggtgagt ttatcgcgaa gttcaacgac cagccgttcg
      541 gcggtttttt tgccaatccc tggaatacca gaaagacggc ggatgtctcc atttttcaac
      601 gctgaaataa gctcatcaat agtgagtcct gagaggatcg tgagggctaa tttgggaccg
      661 acaccagaga cagatatgag tctgataaaa atttcctttt cgagttttgt gctaaacccg
      721 tacaggatca atgcatcttc gcggacgatc atttgaatgt gaagagagat cggtgatcca
      781 ttttcgccag cctgatagaa ggtagagagg gggatgaata cgtcgtatcc aatgcctccg
      841 acatcaacaa taaaattcga tggatttttt tcaagtaatt taccggtcag gaacgcaatc
      901 ataagaaaaa taaattgaag taaaaagaag gtaggtgata ggttggctac accctacttg
      961 aaaccggcaa tgaggatcaa gcgaaatcca ggctgttcga gagagaagcg gtcattgatt
     1021 tatttaatgt gattacatat gttaagatgt agaaggtata tctgtaagca taatttgtaa
     1081 tttgttaatt ttgcgtaatt tattgatatt aattttgtaa atagatggga atacagttat
     1141 gtaatctttg aaattacttg taaatactat attagacgtg acagtaaatc cggctgactg
     1201 gacatcccga gttgaaattc cctgttgttt ggggaaagat ggcgaagact tcaaagatca
     1261 atctcaatct gattctaccc acaaaaaaat gaagctcatg aaacgcattt taattgtcga
     1321 tgacgacatc gaatttcgac aggttttaga gcaacttatg ctccatgaag gtcactgggt
     1381 cagggtggcc gaatcctcag cgaaagccat ccagttattc cgcgaaatgg ccaacacacc
     1441 tcagccattt gatctggtga tttcagatat gatgctgggt gatgggtcag gcctcgatgt
     1501 gctcagtgtt gtcagagaag tgacaacaga catgccgttt atctttgtga ctgcttttgg
     1561 ttcgatggaa tctgttgctg aagcgattcg caaagatgtg tttgactatg tcgctaaacc
     1621 atttgatgct ggtgaattat tgcgggtcgt cagacgtgca ctatcaaagc tttcgactga
     1681 aattgaacca acacttgaag tcccggattc agtggctgga agtctaaaaa tcatcgggag
     1741 cagcccgccg atgattgagc tttacaaggc cgtcgcccgg gtttcggcaa cggactcaac
     1801 cgtattaatg tcaggggagt caggaacggg aaaagaactg gtagcccggg caatccacaa
     1861 taattcattg cgcgcccatc gtccgttcat tggggttaat tgcggtgctt ttcctgaaac
     1921 cctgctggaa agtgaattat ttgggcatgc caaaggttcg ttcactggtg ccaacaccga
     1981 gcgaatcgga atcttcgaag cggcggctgg tggtaccgtt tttttagacg aaatctctga
     2041 aacctcaccc aatttccagg tcaaactcct gcgcgttctg caagagcgcg agctcgatcg
     2101 cgtcggcggc gaacagcccg tcaagatcga cgtccgcgtc atctcggcga ccaacaagaa
     2161 gctcggtgac gaggtcgcgg ccggtcgctt ccgcgaggac ctcttctatc gtttgcaggt
     2221 cgtcccgatc gtgctgccgg cgctgcgcga gcgcctcgaa gatctgccgc tcctcgtcca
     2281 gcacttcatc gcaacgctcg gcccgcgcac caacccaaag gtccgcacga tcgacgaggc
     2341 ggcgctcgag cgcctctccg cgcaccactg gccgggcaac atccgcgagc tcggcaacgt
     2401 catcgagcag g
//
LOCUS       DAOQDO010000887         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39278_length_2411_cov_2.185059, whole genome shotgun sequence.
ACCESSION   DAOQDO010000887 DAOQDO010000000
VERSION     DAOQDO010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39278_length_2411_cov_2.185059"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..2298
                     /locus_tag="PKZ53_13990"
     CDS             334..2298
                     /locus_tag="PKZ53_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41599.1"
                     /translation="MPHPIVDAIRAGKAPKAARLAAAKGILPLPPEDLLTVLSVLIRD
                     QEEEIRAAVPETLAGFNPEVMLSLAHQANTPPDLLFFLCTTPGIPEAVVEAVILNRAT
                     PDEAIVKFALSTQSTHLIEALTINQQRLIRNPQLIDAILHNPKRSPEAERRARETKVE
                     FFEKEYGKQRISQEAAAQSRGTQADAQFTVVAAPPPKPPAGGSMFTGPVADLDLPPLV
                     TATPGAPLAPLPPPPPLATTPAAPPPPPKPAGPKKDIKLSSSPPVAMIQQGRAPRPAK
                     LMTAMGKIPLAPEDLLFAQVLLTQDTDPEIATAALASLNEKDPESLVPFAQNRETPEE
                     VLVFLMTWPKTSVSLGEALLVNASTPDQAVLAFAKMTQSGSLLEAVTINQQRLIRIPE
                     LIDAVLNNPSRTFEAERRAKEVRLEFFEKELGAARIAEEKKARADKFAGITETPFVED
                     EFEALLTEFEAEAGVPISDEAPSVEDALAIFNRMVKDVADTEVVEGVPLPQHLTKERQ
                     KGEEDISRLSIYQQLARMSIKERIFAAFRGNREVRAILIRDANKLISTAVIKNPKITD
                     LEVEAFAAIKGINEDVLRAIAMNRAWISSYNIMHNLIRNPRCPVSFSMQFLNRLQQRD
                     LINLSKSKGIPDMIRQAAGRLATKRKEGMG"
BASE COUNT          624 a          677 c          612 g          498 t
ORIGIN      
        1 ttaaaaaaga aatgacgtga tttcagataa ggttgcgata tacaactctg aatccgaaag
       61 agagtcaaga gatttttggg ctggggccag gggccagggg cagtcgaagg gatgagagat
      121 gaaggatgag ggatgaaata aagccagttc ttcagcccaa tgtcttcagc ccgacttctt
      181 ccgccccaaa attgtcattc ggccactgcc tggagttgtg gcataatgcc ctcttcaaaa
      241 ttccagtagt ccatcgccaa atttccattt tcaaccaaca cgtaccttga gcgacgacag
      301 ccgctcacag gtggaagatc tgactcaaca cctatgcctc atccaattgt tgacgccatc
      361 cgtgccggga aagcgccaaa agccgcccgg cttgccgccg ccaagggtat cctccccctc
      421 ccaccggaag atttgttaac tgtgctttcg gttctgattc gcgaccagga agaagaaatt
      481 cgggccgccg tgcctgaaac actggctgga tttaacccgg aagtcatgct gtcgctggct
      541 catcaagcca atacaccgcc ggatctgctc tttttcttat gcacaacgcc gggcatcccc
      601 gaggcagtcg ttgaagctgt cattttaaat cgcgcgacac ctgatgaggc gattgtcaaa
      661 tttgcgcttt cgacacagag tacacatctg attgaggcgc tgacgatcaa ccagcagcgg
      721 ttgattcgaa acccgcaact tattgatgcc attctccata acccaaagcg atcacctgaa
      781 gccgaacgcc gggcccggga aaccaaagtt gaattttttg agaaagaata tgggaagcag
      841 cgcatcagtc aggaagctgc cgcccaaagc cgtggaacac aggcagatgc tcaatttaca
      901 gttgtcgccg caccaccgcc caagcccccg gcaggtggct caatgtttac cggtccagtc
      961 gcggatttag accttccccc gctggtgaca gccacgccgg gcgcgccgct ggcgccattg
     1021 cctccgccgc caccactggc aacaactccc gcggccccgc caccaccgcc aaagccagcc
     1081 ggcccgaaga aagatatcaa actctcatca tccccacccg tggcaatgat tcagcaaggc
     1141 agagcacccc gtccagccaa gctgatgacc gcgatgggca agatcccgct ggcgccggaa
     1201 gatttgttgt ttgcccaagt gttgttgacc caggatacag atccggaaat tgccacggca
     1261 gccctggctt cactcaatga aaaagacccg gaatccctgg tgccatttgc ccaaaaccgg
     1321 gaaactcctg aagaagtcct ggtctttctc atgacctggc cgaagacctc tgtctcactc
     1381 ggcgaagcct tgctcgtcaa tgcttcaact ccagaccagg ccgttctggc ctttgccaaa
     1441 atgacccaaa gtgggtcact ccttgaagcc gtcacgatca accagcagcg tctgattcga
     1501 atacctgagt tgattgatgc cgttctgaac aatccatcca gaacctttga agctgagcgc
     1561 cgggcaaaag aagtccgact cgaattcttt gaaaaagaac tcggggccgc tcgaatcgcc
     1621 gaagaaaaga aggctcgcgc cgataaattt gctgggatta ctgaaacacc gtttgttgaa
     1681 gatgaatttg aagcactgct caccgagttt gaagctgaag ccggggttcc gatttcagat
     1741 gaagcgccct cggttgaaga cgccctcgcg atttttaacc ggatggtgaa agatgtcgcg
     1801 gatactgagg tagttgaagg tgttccgctt ccgcagcacc tcacaaaaga acgtcagaaa
     1861 ggcgaagaag acatcagtcg cctttcgatc tatcaacaac tggccagaat gtccattaaa
     1921 gagcggattt ttgccgcgtt tcgtgggaac cgtgaagtcc gggcgatttt gattcgggat
     1981 gccaacaaac tgatttcaac cgccgtgatc aagaacccca agatcaccga tctggaagtg
     2041 gaagcatttg cggcaattaa gggcattaac gaagacgtgc tgcgagcaat tgccatgaac
     2101 cgtgcctgga tcagttccta taacatcatg cataacctga ttcggaatcc acgctgcccc
     2161 gtgagcttca gcatgcagtt cctgaatcgc ctgcaacaac gtgacctgat taacctgagt
     2221 aagagtaaag gcattccgga tatgattcgt caggcggctg gacgactggc taccaaacgc
     2281 aaagaaggaa tgggatgaca ggtgactggg tgacaaggtg acaaggtgta agggcgctgt
     2341 tgctttgaac cgcgccccct tcgaaaaccc ggaacccgga acccggaacc cggaacccgg
     2401 aacccggaac c
//
LOCUS       DAOQDO010000888         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39385_length_2407_cov_3.507228, whole genome shotgun sequence.
ACCESSION   DAOQDO010000888 DAOQDO010000000
VERSION     DAOQDO010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39385_length_2407_cov_3.507228"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="PKZ53_13995"
     CDS             complement(<1..1592)
                     /locus_tag="PKZ53_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ41600.1"
                     /translation="MYTRIVKKQGFAAIGVFLVMSLLLVSSSLWPSAANPALTASSPR
                     TITPLLRGATPQGPAVIGQSDLQGTSISYRNAGGLALDSSVQPSRLYVADTSNNRVLG
                     WASVKDFQAGAAPGIVLGQPDFGTLAANSRGISSESLSHPVGVAVDKAGNLFVADAGN
                     NRVLRFDSPFTTDTAADLVWGQDSFTDNQPRSKRAGFDYPTGVAVGTTTANGEFLYVT
                     DQGNKRVVVYHTTDTTPVNVLENEAFKSPAGIAVTQFFEELFVTDSGTNKVFVYTDAS
                     TTPTLTRVIDKVGKKVSKDASKIPFKGIGAIAASPTGGFYLADSMNGRVIEFTTPTNE
                     EDFDADRIFGASDADLYSPFAKNFRGMPLAVAIDRDGSIYVSDASSGSIQVLPSPNAP
                     VSQSLPTDSSKGGLDVNPNAFPGTDTFNPAGSYVRVPTGINNLWQITISSAAAACTPG
                     TGGDPINNAGHTGPNCAGFRNNASCSIASPNVSISAGELRSGTGANVCTDYNLTGICG
                     GATLNLNYFLRTAPAAFGTRQA"
BASE COUNT          569 a          599 c          660 g          579 t
ORIGIN      
        1 aatgcctgcc gggtgccaaa cgccgccgga gcggtgcgca ggaagtagtt caggttcaat
       61 gtggcgccgc cgcagatacc ggtcaggtta tagtcggtac acacgttcgc gcctgttcct
      121 gaacggagtt caccagccga aattgagacg ttggggctgg caatcgagca gcttgcattg
      181 ttgcggaagc cggcgcaatt tggtccggtg tgacccgcat tattgattgg gtcaccaccc
      241 gtgcctgggg tacaggcggc ggcggctgat gaaatcgtga tctgccacag gttgttgatc
      301 ccggttggta cacggacata gctgccagcc gggttgaagg tatctgtccc tgggaaggca
      361 ttcggattga catccagccc gccttttgac gaatcagtcg gcaatgattg gctgactggt
      421 gcattcgggg aagggaggac ctggattgat ccggaactgg catcgctcac ataaattgag
      481 ccatcccggt caatcgccac ggccagtggc attccacgga agtttttggc gaaggggcta
      541 tagaggtccg catcgctggc gccgaaaatg cggtcggcgt caaaatcttc ttcgttggtt
      601 ggggtggtaa attcgatcac ccgtccattc attgaatcag ccagataaaa accgccggtt
      661 ggagatgcgg cgatggcgcc aattcctttg aatggaattt tggacgcatc cttgctgacc
      721 ttcttgccaa ccttgtcaat gacgcgcgtc agggttgggg ttgtggatgc atcggtgtaa
      781 acgaaaacct tgttggtccc ggaatcggtg acaaacaatt cttcgaagaa ttgggtgact
      841 gcaatgccag ccggagactt aaaggcttca ttttcaagca cattgactgg ggtggtatct
      901 gttgtgtgat agaccacaac ccgcttgttg ccttgatcgg tgacatagag gaactcacca
      961 tttgcagtgg tggtgccaac ggcaacaccg gtcggatagt caaatccggc ccgtttgctc
     1021 cgcggctggt tgtcagtaaa actgtcctgt ccccagacga ggtctgccgc agtgtcggtg
     1081 gtaaatggcg aatcaaaccg cagaacccga ttgttgccgg catcggccac aaacaggttg
     1141 ccggctttat caaccgcgac cccgactgga tgagagaggc tttcagaact aatgccacgg
     1201 ctattggcgg caagtgttcc aaaatcaggc tgtcccaaaa cgatgcctgg agcagcaccg
     1261 gcctggaaat ctttcacact tgcccaacca agtacccgat tattcgaggt atcagccaca
     1321 tacaatcggc tgggctggac acttgaatct aaggcaagcc caccagcatt gcgatagctg
     1381 attgaggttc cctgtagatc actttgacct ataaccgcag gtccttgcgg agtggcacca
     1441 cgcaaaagcg gggtgatcgt ccgtggggaa gaagcggtca aggccgggtt agcagcggag
     1501 ggccagaggc tgctgctaac cagcaatagg gacatcacca ggaagacccc tattgccgca
     1561 aaaccttgtt tcttcacgat acgtgtgtac attgacgttt ctcctctgtt gtttctgcga
     1621 cctaactccc aagaaagcag gactcaactg ccaggcagtc atcctgcgga ctttacctga
     1681 aagccgagca ggtcggactg atcggagttg aactgtggta ggttttcccc ttgtcgcatt
     1741 tcaattgaag ataccatcca agcatcacaa aaagagtatc ttattaaagg ctcactcttg
     1801 attgagctgg gccgaggatg cggggttttt gggtagaaca cgggtgatag ataccgcttt
     1861 caacttcagc cccgggacgt tctgtccagg accgtttaga aatagtacat gccctttggc
     1921 aggtgtttcc cgcctggggg aatttccctg ggttggtgct gaattggtct accacttcgg
     1981 gaaagggttt gatcttcccc tgacgaattg aagagaaggg gcacagatgg atgctgtgcg
     2041 agatgcaaga accaaaaagt gtgttttgaa tgagatggtt attgttaaaa ggctgaggtg
     2101 actacctagc gcaaccttct cacactcgca aattgcttgt caagcagtcc gaaaaagtca
     2161 gaaactgaga cgtttttcca gtctcagtcc ataaaaacca caaagtaact gtttcaggaa
     2221 ttgaaaagtg cgaatggagc atgttttgtt tagcaaacct cataccacgt ttgcccatca
     2281 cagcgcgtga aacaacttga cagcgccttc ttttcccttg tactttgcca ctctcgcttc
     2341 actttccatc cttttccgat caagctgaag cagcctgcat tcatttcaat cacagaaggt
     2401 ggtgata
//
LOCUS       DAOQDO010000889         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39417_length_2406_cov_3.286687, whole genome shotgun sequence.
ACCESSION   DAOQDO010000889 DAOQDO010000000
VERSION     DAOQDO010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39417_length_2406_cov_3.286687"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..970)
                     /locus_tag="PKZ53_14000"
     CDS             complement(50..970)
                     /locus_tag="PKZ53_14000"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNJ41601.1"
                     /translation="MLAGSKFTSQLFKTITSLVQWIDSDFASKKLKEMDLPDRVDLIR
                     TIPFIALHLMCLAVFIVGWSWTAVMTAMALYFVRMFAITGFYHRYFSHKTFHTSRIAQ
                     FLFAVLGASAVQRGPLWWAYIHRHHHQHSDKEGDFHSPIQRGFWWSHIGWITSPRNFP
                     TDYNRVRDLAKFPELVFLNRFDTLVPTVLAVGLFFFGKVLEWYVPGAGTTGAQMLVWG
                     FFVSTTVLFHATASINSIAHLFGRRRFQTGDWSRNNFLLALITLGEGWHNNHHKYMST
                     MRAGIRWWEIDITFYVLKMMSWMRIIWNVK"
     gene            1278..2168
                     /locus_tag="PKZ53_14005"
     CDS             1278..2168
                     /locus_tag="PKZ53_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNJ41602.1"
                     /translation="MIPLRLELSGLPHHHRLHRLLASRTPQTSTRAEVTDQIPFWAAS
                     FFQLDQSRLFQQSTPDEQSAILTGCSRAILEEAFWIEKCGMYFAPKMALLAQSPETQM
                     LYCQFAAEEASHFQLVSQFLNVAAISPPVANPFITFLAQILETESPGVLQFFIQVVLE
                     GWGIAHYRTLSKACLNPGLKAVFEQILKDEAHHHGSGVILCSEQAFATTELSRIEELV
                     AGLLQMIKIGPAMVISQVEQIKGHLSPAQKKQLWSDLKAEEATRHKLQLIQNLMHLVP
                     ATSHLIETLDAKGWFSLSAR"
     gene            2144..>2406
                     /locus_tag="PKZ53_14010"
     CDS             2144..>2406
                     /locus_tag="PKZ53_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41603.1"
                     /translation="MVFTFRPVTCLAPDPANQILTMNQTLDYSLPHASLRDDQKTCRK
                     LELNLRKNRDQDSTQEIDRLAAQFDFQTCHDQYWNPEAFSLLFA"
BASE COUNT          647 a          639 c          561 g          559 t
ORIGIN      
        1 cgctgttttc agcccaattt cttcatcccg ctggttgaag ccccagcctt catttcacat
       61 tccagataat ccgcatccag gacatcattt tcagcacata aaacgtgata tcaatctccc
      121 accagcgaat gcctgctcgc atcgttgaca tatacttgtg gtgattgttg tgccaccctt
      181 cgcccaacgt aatcaacgcc agcagaaaat tgttgcggct ccagtccccg gtttgaaacc
      241 gacggcgacc aaagagatgc gcaatggaat tgattgaggc cgtggcgtga aagagcacag
      301 tggtgctcac aaaaaacccc cagaccagca tctgggcccc ggttgtacca gcccctggaa
      361 cataccattc caacactttc ccaaagaaga acaacccaac tgccagcact gtcggaacaa
      421 gcgtatcaaa ccggttgagg aagaccagtt ccggaaattt ggccaggtcg cgaacccggt
      481 tatagtcagt tggaaaattc ctgggactgg tgatccagcc aatatgcgac caccaaaatc
      541 cgcgctggat aggcgaatga aagtcacctt ctttgtctga atgttgatgg tgatgacgat
      601 ggatatatgc ccaccagagc gggccgcgct ggacggctga agccccaagc acggcaaaca
      661 ggaattgggc tatccgggaa gtatgaaacg tcttatgtga aaaatagcga tgatagaacc
      721 cggtgatggc aaacattctg acaaaataca gtgccatcgc tgtcataaca gccgtccaac
      781 tccaccctac aataaacacc gccagacaca tcaggtgcag cgcaataaac ggaatggtcc
      841 gaatcagatc aacccgatca ggtaagtcca tttccttgag ttttttactg gcgaagtcag
      901 aatcgatcca ttgaaccagt gaggtaatgg ttttgaacaa ctgagaggta aattttgatc
      961 ctgcaagcat acgcgacaat caaaagagga acgggagtag gtaggggcga acctttaaaa
     1021 tcatccaggt tcattgccaa tttacacgga aaaactgacg tgatgagaaa ttcttggggg
     1081 tacggttggc aaaatggaat gaagttcgct atttagcata ttccatctga cgtggctagt
     1141 cgtgtttgtc aatattgaac aactgtcaca cctgctcttg acataagata cgagagcacg
     1201 aaaccctgta cactgcttgc tttcagatga cgggtttaat cagttgcctc tgacacagcc
     1261 tttgagacag aaagactatg attccacttc ggttagagct ttccgggtta ccccaccacc
     1321 atcgcctgca ccggcttctg gcgtcgcgaa ccccacagac cagcaccagg gctgaggtta
     1381 ccgaccagat accgttttgg gcagcttcat tttttcaact tgaccagtca cggttatttc
     1441 agcaatcaac acctgatgag caatcggcga tcttgaccgg gtgcagtcga gccatcctgg
     1501 aagaagcatt ctggattgaa aaatgcggga tgtattttgc cccgaagatg gcgctcctgg
     1561 ctcaatctcc ggaaacccag atgctctatt gccagtttgc ggctgaggaa gcctcccatt
     1621 ttcaactggt ctcacaattt ctgaatgtcg ctgccatttc accgccagtc gcaaacccct
     1681 ttatcacatt tttagcccag attctggaaa ccgaatcgcc aggtgtcctg caatttttca
     1741 ttcaggttgt gcttgaaggg tgggggattg cccactatcg aaccctctcc aaagcctgct
     1801 tgaatccagg tttgaaggca gtttttgaac aaatcctcaa agatgaagcc caccaccacg
     1861 gcagcggggt cattctttgc tctgaacaag cgtttgcaac cacagagttg agccggatcg
     1921 aagaactggt tgcgggactg cttcaaatga tcaaaattgg acccgccatg gtgatcagtc
     1981 aggttgagca gatcaaaggc catctgtcgc cggcccaaaa aaagcaactc tggtctgatc
     2041 tcaaggctga ggaggcaacc cggcacaagc tccagctcat tcaaaatctg atgcacctgg
     2101 ttcccgcaac ctcacatctc attgaaaccc ttgacgcaaa gggatggttt tcactttccg
     2161 cccggtgacc tgcctggcac ccgacccggc aaatcaaatt ttgactatga accagacgct
     2221 ggattattca ctgccgcacg cttcactccg tgacgaccag aaaacctgtc gtaaattgga
     2281 gctgaacctc cggaaaaatc gtgaccagga tagcacccag gaaatagatc ggctggcggc
     2341 gcagtttgat tttcagacct gtcacgatca gtactggaac cctgaagcct tttcactgct
     2401 ctttgc
//
LOCUS       DAOQDO010000890         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39418_length_2406_cov_3.230966, whole genome shotgun sequence.
ACCESSION   DAOQDO010000890 DAOQDO010000000
VERSION     DAOQDO010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39418_length_2406_cov_3.230966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1036)
                     /locus_tag="PKZ53_14015"
     CDS             complement(47..1036)
                     /locus_tag="PKZ53_14015"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNJ41604.1"
                     /translation="MPGKTILEIPEAEQAEMLKELRRHRYGYLLALHVLILCARGYNP
                     TVIAEVLLCSRTSVYRIVEAYRKGTLDIFDAEGRISRPLRTTVLIPKIKESIVAMLKK
                     APEVYGWCRTRWSCETLALELKAKSGIEVSAETMRRWLKEIGWVWKRAKLVAKDTDSA
                     RITKLAHIRLAWEQACTRTAFFFADELDLHLLPKVGYEWTLKGTQTEVMTPGQNQKTY
                     LAGALDFKTGQVHTHLSDHKNGELFLSLLTSLDAAYPVTDFDRIVIVVDNFKIHDSVK
                     VNTWLETHPRFERLFLPTYCPRANPIERVFGDVHDQCPQSQTFFPDSSRGGRA"
     gene            1155..>2406
                     /locus_tag="PKZ53_14020"
     CDS             1155..>2406
                     /locus_tag="PKZ53_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41605.1"
                     /translation="MEQALGYYNKALPLAESLAEKEDKDQLFAVFLNTGRTLTLLCRF
                     REAREHYNQALNLTKKMSDIDQAKVLLNFVEIDYLCGDDSSAKSRLDKIFDLLQSVDV
                     SSEKATVKANAYLSRGVQAWEKNRNLNQALQDFDEAERVNPQTDDRDFQSVLDYNRGF
                     VYYVDGEANPESYEKAETCFRSAHDKASNEEFWNPARLADSLRGLALVHSKQKKYTEA
                     IKEIEEAINIFHFKRSQAKSFESRAFLMNFGRQYYESYLEIFFEAQKAQKGADFVESV
                     FLKSDLAHSLSFFELLQESKQRGKEVPLSMSQREALDKGSPMPANLEDQARKLGSEYL
                     PHPLPTLSQLQKSLCSDGKTVGLEYWLGVQKSYVWVITDTSIKLYELPPREKIEEATR
                     TLIKGCNPTLESGFVSTEKVKELAL"
BASE COUNT          665 a          553 c          556 g          632 t
ORIGIN      
        1 aaattccgag agcaggtact tccagagacc attcacctga aagtgatcaa gcacgtccgc
       61 cacgacttga gtcaggaaag aacgtttgtg attgcggaca ttggtcatgg acatcaccaa
      121 aaacgcgctc aatcggattg gccctgggac aataagtcgg taaaaagaga cgctcaaagc
      181 ggggatgtgt ctcaagccag gtattgactt tgaccgagtc atgaattttg aaattatcaa
      241 ccacgatcac gatccgatca aagtcagtga ccgggtaggc ggcatccaac gacgtcagaa
      301 gcgaaagaaa taactcgcca tttttgtgat cactgaggtg ggtgtgaacc tgtccggttt
      361 tgaaatcgag tgccccagcc agatatgttt tttgattttg tccaggggtc atcacttcgg
      421 tttgagttcc tttgagcgtc cactcatatc ccaccttcgg gagcagatgc agatccagtt
      481 cgtcggcaaa gaaaaaggcc gttcgggtac aggcttgctc ccaggcgagg cggatgtggg
      541 ccagtttggt gatcctggct gaatctgtat ccttggccac caacttcgcc cgtttccaga
      601 cccatccgat ttctttcagc caccggcgca tcgtctccgc cgaaacttcg attccgcttt
      661 tggccttgag ttccagagcc agggtttcac aactccaccg cgttcgacac cacccataga
      721 cttcaggggc ctttttcagc atggccacaa ttgattcttt gatcttcggt atcaacaccg
      781 tggttcgcag tggccgactt atccttcctt ctgcgtcaaa aatgtcgagc gttcctttgc
      841 gatacgcctc cacaattcga tacacacttg tgcgcgaaca caacaacact tccgcaatca
      901 cggttggatt gtatccccgt gcgcacaaga tcaacacgtg cagggctaac aaatatccat
      961 atcgatggcg tcgcagttct ttgagcatct cggcttgctc tgcttcaggg atttccagta
     1021 tcgtttttcc aggcacagat gactttgctc cttggttggt tattttcttt tttctcaagt
     1081 atcaaagttt ttggtctgtg ccaagtcttc tttctctgtt ccaatccctt gtggaccgat
     1141 ttaggcaagg aaagatggaa caagctcttg ggtactataa taaagcctta cctttagccg
     1201 aatcactggc tgaaaaagaa gataaagatc aactctttgc ggtattcctg aacacagggc
     1261 ggacgttgac attgctgtgt cggtttcgtg aagccaggga acactataac caggccctta
     1321 acctgaccaa aaaaatgtcg gatattgatc aagcaaaagt acttttaaac tttgtggaaa
     1381 ttgattacct ctgcggggat gatagctctg ccaaaagcag attagacaaa atctttgatt
     1441 tactccaatc tgtagatgtc agttcagaaa aggcaacggt aaaagcaaat gcctatttat
     1501 ctcgtggagt tcaagcttgg gaaaaaaata ggaaccttaa tcaagctctc caagactttg
     1561 atgaggcaga acgagtaaac ccacaaacgg atgacaggga ttttcaatca gtgctggact
     1621 ataatcgagg ctttgtttat tacgtggatg gtgaagccaa tcctgagtct tatgaaaaag
     1681 ctgagacatg tttcaggagt gctcacgata aagccagcaa tgaagaattc tggaatccag
     1741 ctagattggc tgactctttg aggggattgg cactggttca cagcaagcag aaaaaataca
     1801 cagaggcaat caaggaaatt gaagaagcta tcaacatttt ccatttcaaa cgaagtcagg
     1861 caaagtcttt tgagagtcgc gccttcctca tgaatttcgg tcggcaatac tatgagagtt
     1921 atttggaaat cttctttgaa gcccaaaaag cccaaaaagg ggcagatttt gtcgaatctg
     1981 ttttcctgaa atctgatttg gcccactcgc tcagtttctt tgaactctta caagagtcaa
     2041 agcaacgagg gaaagaagtt cccctgtcca tgagtcaaag agaagctttg gacaagggct
     2101 caccaatgcc tgccaatctt gaggaccaag ccaggaaact ggggagcgaa tatttaccac
     2161 atccgcttcc aaccctttcg caacttcaga agtcactgtg cagtgatgga aaaacggttg
     2221 gtttagagta ctggttggga gttcagaaaa gttacgtgtg ggttattact gatacctcga
     2281 ttaaactcta tgaactccct ccaagagaga aaattgaaga ggcaacgcgt actcttatca
     2341 aaggttgtaa tccaactctt gagagtggat tcgtctctac tgaaaaagtc aaagaacttg
     2401 ccctgg
//
LOCUS       DAOQDO010000891         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39484_length_2404_cov_2.891443, whole genome shotgun sequence.
ACCESSION   DAOQDO010000891 DAOQDO010000000
VERSION     DAOQDO010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39484_length_2404_cov_2.891443"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1999)
                     /gene="thrS"
                     /locus_tag="PKZ53_14025"
     CDS             complement(59..1999)
                     /gene="thrS"
                     /locus_tag="PKZ53_14025"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNJ41606.1"
                     /translation="MSSQSEVSSVVESGPLTALVVLSQRDRETAKRAIAARVNGEAVD
                     LTRLVTSTDTVEAITTDSPEALEIYHHSTAHLLAAAVLELFPGTKLGAGPALHDDPKG
                     GFYYDFLQAQTFTPEDLTRIEKKMRELVKRNLKYERVEIAREAALQQFSADELKCYFI
                     SEKGGENPSFYKLGDHFIDFCRGPHIPSTAKIKAFKLLSVAGAYWLGDEKGPQMQRIY
                     GTSFFSQEELDAWIHQREEAAKRDHRRVGRELDLFSFTEQIGSGLPLYHPKGGLMLHL
                     LQEYIFSELLAHGYSLVKTPHVASRALWHVSGHEENYQESMFPVMKFEDESVEYRLKP
                     MNCPFHIQIYKSQQRSYRDLPQRYAEMGTVYRYERSGVQHGLLRVRGFTQDDAHLFCT
                     PENLLDEIVSCLEFARTIYTTFGFDYKIELSVRDAEKTAKNYLGSDEVWEVAESALTE
                     ALNKVGLPYIRVEGEAAFYGPKIDVKVVDAIRRTWQLATIQVDFNLPRRFNLEYIGAD
                     NQPHTPIMVHRAILGSLDRFFGVLIEHFGGRFPLWLSPVQAVVLPITDRVNDCASRIG
                     DRLKRAGIRVEVDLRGEKIGAKIRQAQLQKIPYMLVIGDREAEDGTVAVRTRAQGDLG
                     TMPLEQFIAQATDEVRQRVLTA"
BASE COUNT          554 a          656 c          648 g          546 t
ORIGIN      
        1 agcgtcgtgg gtacacgact caagcttggc ccttgagggc cgagtctctt gactcggttt
       61 acgctgtcag cactcgttgc cgaacttcgt cagtggcctg ggcaatgaat tgctcaagcg
      121 gcatcgttcc aagatctccc tgggcgcgtg tccggacggc gaccgttccg tcttcggctt
      181 cgcggtcacc aatcaccagc atgtagggaa ttttttgcaa ctgggcctga cgaatcttgg
      241 cgccgatctt ttccccacgc agatcaactt cgacgcggat cccagctcgt ttcaacctgt
      301 cgccgatacg gctggcacaa tcgttgaccc ggtctgtaat cggaagcacg acggcctgaa
      361 ccggtgataa ccagagcggg aatcgcccac caaaatgctc aatcaacacg ccaaaaaatc
      421 ggtccagtga acccaaaatc gcccgatgga ccataatcgg cgtgtgcggc tggttatcag
      481 cgccgatgta ttcgaggtta aagcgtcgcg gcagattaaa atcaacctgg atcgtcgcca
      541 gttgccaggt ccggcgaatg gcgtccacca ctttcacgtc aattttcgga ccatagaacg
      601 cggcttcccc ttcaacgcga atatagggca atccaacttt attcagcgct tcagtcagcg
      661 ctgactccgc aacttcccag acctcgtcgc tgccaaggta atttttggct gtcttttcag
      721 catcccgaac cgaaagctcg attttgtagt caaacccgaa cgtcgtgtag atggtgcggg
      781 caaattcaag acaggacacg atttcatcaa gtaagttctc tggagtacag aacagatgcg
      841 catcatcctg ggtaaatccg cgcacccgca gcaagccgtg ctgaacccct gagcgttcat
      901 atcggtacac tgtccccatt tcggcatagc gctggggcaa atcgcgatag ctgcgctgtt
      961 gtgatttgta gatttgaata tggaacgggc aattcatcgg tttgagccga tattcgaccg
     1021 attcatcttc aaacttcatc accggaaaca tgctctcctg atagttttct tcgtgaccgg
     1081 agacgtgcca cagcgcccgc gaagcgacgt gcggggtttt gaccaggctg tacccgtggg
     1141 ccaggagctc gctaaagatg tactcctgca gcaaatgaag catcaggccg cctttgggat
     1201 gatagagcgg cagcccagag ccaatttgct cagtaaagct aaacaaatcg agttcgcggc
     1261 caacccggcg gtggtcgcgc ttggcggctt cttcgcgctg gtgaatccag gcatcgagtt
     1321 cttcttgtga aaagaaggac gtgccataga tgcgctgcat ctgcggacct ttttcatcgc
     1381 cgagccagta ggcacccgcc accgacagca atttaaaggc tttgattttg gccgtgctgg
     1441 gaatatgcgg cccacggcag aaatcaataa aatggtcgcc cagtttgtag aagctagggt
     1501 tttcaccacc tttttcgctg atgaaataac atttcagttc gtcagcgcta aattgttgca
     1561 aggcggcttc acgggcaatt tcaacgcgtt cgtacttgag gtttcgcttc accagctcgc
     1621 gcattttctt ttcaatgcgg gtcaggtctt caggcgtgaa ggtttgagcc tgtaaaaagt
     1681 cgtaataaaa cccacccttc ggatcatcgt gcagcgccgg tccagcccca agcttggtgc
     1741 caggaaacaa ttcgaggacg gctgccgcca gcaaatgggc ggtcgagtgg tgatagattt
     1801 cgagtgcttc tggggaatct gttgtgatgg cttcaacggt gtcggtggat gtcaccaacc
     1861 gggtcaggtc gacggcttcg ccgttgacac gggctgcaat ggcacgtttg gccgtttcac
     1921 gatcacgctg agacaatacg accagcgccg tcaaagggcc ggattcgacc actgaagaca
     1981 cttcgctctg tgaactcata acctcttaca ttcctcgctg actggctctc ccccgacgaa
     2041 tcagacccaa acgcgagcct ggcctcatca aaggcgacca atcacactcc aaaactgaag
     2101 tcaaaaaatt gaattatcgg ttgcgcggta agattcctcg atcagcgtct caagggggca
     2161 acacggggtt taagcgtgtg gcgggcttgc cggaaagcgc gagtggagag gcaacgccgg
     2221 gagagtggcg tttggtgtgc cctccgtgtg gatgggtgcg gcaagcgaca cagattcgag
     2281 aatgcaatct gtgtaaaacc ttgagctctt ctggagctga cgtgagaatg aaccagcagt
     2341 gaccaccacc ctaaaaaagg atggtagacg cgagcggatt tgaaccgctg acccctaccg
     2401 tgtc
//
LOCUS       DAOQDO010000892         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39488_length_2404_cov_2.793103, whole genome shotgun sequence.
ACCESSION   DAOQDO010000892 DAOQDO010000000
VERSION     DAOQDO010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39488_length_2404_cov_2.793103"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..>2404
                     /locus_tag="PKZ53_14030"
     CDS             148..>2404
                     /locus_tag="PKZ53_14030"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNJ41607.1"
                     /translation="MIVPVHQGLTAIDPGLPSSGNRNLDCRPEASQRTTVKPQTGQLP
                     MIFEPNLGQVSGDLDYMARGVGYTLGITDQELVLALNRSTNRTSPVAEQVTLRMQFRG
                     SRPAQAVTAEERQPTVKHYLRGNDPAGWHRNIPTYGRVRYQGIYPGVDVVYYGREHHL
                     EYDLEVKPGGDPGVIRIRLAGASRLSLDTAGNLMVNVGGGKLRQPRPVAYQEINGSRQ
                     HVEASYRLHPGQELSLALGVYDQRYPVVIDPVLEYATYLGGTEAEAGYRTAIDGEGNV
                     YVVGMTRSPGFPVQAGLQATSGGDLDVCVAKLNAAGTGMVYATYLGGDGTDAAHGLLI
                     DPNSGDITVTGVTRSTNFPTTETAYQPTLAGGYDAFVAQVSSDGSQLAYSTYLGGSRD
                     EMANALAGGDRQFYIAGWTESTDMLGRPGQVQATNHGRRDAFVLRFAPTNTEFSPVEY
                     FSYLGGTGQDLAYGVAVDASSRIIVTGSTRSADFPIENAIQPTYGGGVGYFGDGFVAV
                     LEGSDLVSSTYVGGSGSDACFGVTQNNIGEVYVTGMTASVDFPVINGLQATVQGESDA
                     FLIKLNLANSPPQVVYATPIGGSGNEQATQVIAANSGAIYLSGRTNSVDFGDRTQVES
                     PGGNWDVAVIKLNEVNAGTQPTLVTAVVFGGTEADTGESIALGTAGTVVVAGVTQSAD
                     FVTANAIQPATGGNQDAFIVKLRLCAEVVLQPATLPVGTVNEPYSQVLTAEGGIAPYR
                     FEVTEGSLPGGF"
BASE COUNT          554 a          556 c          774 g          520 t
ORIGIN      
        1 ctggtattcg gggatgggat caacgggtga aaaaatccag tttacttata aaaagcagga
       61 atcattgtca tggcattaca accgggaaac cagccgcggc tgaaagtttg gtcattttgt
      121 ttggtccttg ttcttggagt atggggaatg attgtgccag ttcatcaggg gctcaccgcc
      181 attgatccag gactcccgtc gtctggaaac aggaatctgg attgccggcc tgaagcaagt
      241 caacgaacca ccgtcaagcc ccagacaggt caactgccga tgatatttga accaaatctg
      301 ggacaggtgt ctggtgatct ggattatatg gcacggggtg ttgggtatac acttggcatt
      361 acagatcagg aactggtgct ggcattgaac cgatccacaa accgaacttc gcccgtggca
      421 gagcaggtca ctctgcggat gcaatttcgg ggaagccggc cagcgcaagc cgtcaccgcg
      481 gaagaacggc aaccaacagt caaacattat ctgcgaggaa atgacccggc ggggtggcat
      541 cggaatatcc caacctatgg gcgggttcgg tatcagggga tctatcctgg ggtggatgtt
      601 gtgtactatg gccgggaaca tcatctggaa tacgatctgg aagtgaagcc agggggtgat
      661 ccgggggtga ttcggattcg gctggctggt gcgtcgcgac tcagcctcga cacagccggg
      721 aacctgatgg tgaacgttgg cgggggaaag ttgcggcaac ctcgaccggt tgcctaccag
      781 gaaatcaatg gaagccggca acacgtagag gcgagctacc gactgcaccc tgggcaggaa
      841 ctcagtttgg cgttgggagt ctatgaccag cgatatccgg tcgtgattga tccagtgctg
      901 gaatatgcaa cctatttggg gggaaccgaa gcggaagcag ggtatcggac agcgattgat
      961 ggcgagggaa acgtgtatgt ggtgggaatg acccgctcac ctggatttcc ggttcaggcg
     1021 ggtttgcagg cgaccagtgg cggggatctg gatgtatgtg tggctaaatt aaatgccgcc
     1081 ggaacgggga tggtctatgc gacctatctc gggggagatg gaactgatgc cgcccatggc
     1141 ttgttgattg accccaattc gggtgacatc accgtgaccg gtgtgacccg atcaaccaat
     1201 tttccaacca ccgaaactgc ctaccagcca accctggcgg gtgggtatga tgcatttgtg
     1261 gctcaggtat cgagcgatgg cagccagctt gcctacagta cctatctggg aggcagtcgg
     1321 gatgagatgg ccaatgctct ggccggaggg gatcgccagt tttatattgc cggttggacg
     1381 gaatcaactg acatgctggg acgcccggga caggtacagg cgaccaatca tggccggcgg
     1441 gatgcgtttg tcctgcgctt tgcaccaacg aataccgaat tctcacccgt tgaatatttt
     1501 agctatttgg ggggaaccgg acaggacctg gcgtatgggg tggcggttga tgccagttca
     1561 cgaatcattg tcaccggaag cacccggtcc gccgattttc cgattgagaa tgccatccag
     1621 ccaacctatg ggggaggagt tggatatttt ggggatggat ttgtggcagt gctggaaggt
     1681 tcggatctgg tgagttcaac ctatgtcgga ggaagcggga gtgatgcgtg ttttggggta
     1741 acccaaaaca atatcggcga agtgtatgtc acaggaatga cagcttcagt ggattttcca
     1801 gtgatcaacg gacttcaggc aacggtccag ggtgaatcgg atgcctttct gatcaagctg
     1861 aatctggcca attcaccgcc tcaggtggtg tatgcgaccc caatcggagg gagcgggaac
     1921 gagcaggcaa cccaggtcat tgccgccaat agcggagcga tttacttgag tgggcggacg
     1981 aattcggttg attttgggga tcggacccag gtggaaagcc caggtgggaa ctgggatgtc
     2041 gcggtcatca agctcaatga agtcaatgcg ggaacccaac caaccctggt cactgccgta
     2101 gtttttggag gcaccgaagc tgatacggga gaaagcattg ccttgggaac ggctggaacc
     2161 gtcgtggtgg cgggggtgac gcaatcagcg gactttgtga cggccaatgc catccagccg
     2221 gccaccggag gaaatcagga tgcatttatt gtcaaactgc ggttgtgtgc tgaagtggtg
     2281 ctgcagccag caacattgcc ggtcggaaca gtgaatgaac cctatagtca ggtgctcacc
     2341 gctgaggggg gaatcgcgcc gtacaggttt gaggtaaccg aaggtagcct gccgggaggg
     2401 ttct
//
LOCUS       DAOQDO010000893         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39566_length_2401_cov_3.547741, whole genome shotgun sequence.
ACCESSION   DAOQDO010000893 DAOQDO010000000
VERSION     DAOQDO010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39566_length_2401_cov_3.547741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKZ53_14035"
     CDS             complement(<1..1322)
                     /locus_tag="PKZ53_14035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41608.1"
                     /translation="MPGTTLIDGKYRILSCLGRGGMGTVYRAEHVHLGRQFAIKTVLP
                     DFASRDPTAIERFRQEARASAAIHHLNVIGVTDFGVTDQRVFYLVMEYVEGRGLHEII
                     NSEGPISPERTLKIMKQVCSGVSAAHALNMIHRDLKPSNIMISRPKQSGVSLEDMGLV
                     TGEDLFPSAPTSGSQSGSQSGNSSDESVVKVLDFGLAKILNSEVLGDGPATLGTGVMG
                     TPHYMSPEQCNGKVVDPRSDVYSLGVVLFQMLTKEVPFKGDSFGAIVSGHLLKNPPSL
                     RTLNPVISEALEMVVLRAMSKDPANRQQSVAVLASELEAAVLNTGQPVANQGAVLMVS
                     TQPGNCDVYVNNDFRGKTGPTGKLAIRLEPGEYRLRFSCPGWFDTSRTLNMGIAEQQI
                     EVALVHKTATDAPRQMSPVTASVPSSTARVQRAPVTGGYATPARGMPAL"
     gene            complement(1668..>2401)
                     /locus_tag="PKZ53_14040"
     CDS             complement(1668..>2401)
                     /locus_tag="PKZ53_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41609.1"
                     /translation="TMSVTNFQAERQQEQELELIQRGNSIAEAIARYNNSGRLGQLNP
                     GGLFVTKLEDLAKGVDINGRLVHFLRPSALRDPMANGREWRALRVGDPLLNRYLQEWA
                     AYYRQRIPDNYRVLVNPIDFKDEDDEDDEDDEDETTDSDDEDDDDEDEDEDTSSSGGT
                     STTGEPSDGKDQSAFGIDQERLPIVGVVSTSRKQAFQRQNGRDKRYDEFVFIYLPAPQ
                     LVREVPAFQDETSDQPSSQSPQEQR"
BASE COUNT          566 a          652 c          575 g          608 t
ORIGIN      
        1 agagccggca ttccccgagc cggtgtggca tatccacccg tgaccggtgc ccgctgtacc
       61 cgagcggtgc tgcttgggac cgaagccgta actggggaca tttgacgagg ggcatcggtt
      121 gcagttttat gcaccagagc gacttcaatt tgttgttcgg caatccccat gttcaaggtt
      181 cgtgaggtat caaaccaccc tggacaacta aaccgtagcc gatattcacc aggctctaat
      241 cgaatggcga gtttcccggt gggtcctgtt tttccacgaa aatcattatt gacgtagacg
      301 tcacaatttc caggttgggt gctgaccatc agtacggcgc cctgattggc aacaggttga
      361 ccggtattga gaaccgccgc ttcaagttct gaagccagaa cagccactga ttgttgtcga
      421 ttggctggat ctttggacat tgcgcgcaag acaaccattt ccaaggcttc ggaaatcacc
      481 gggttgagag ttcgtagact gggagggttc ttcaacaggt ggcctgaaac aattgcccca
      541 aatgagtccc ccttaaacgg aacttctttg gttaacatct ggaagagaac gaccccaaga
      601 ctatagacat cagagcgtgg atcaaccact ttcccattgc actgttcagg cgacatgtaa
      661 tgaggcgttc ccatcacccc agttcccaat gtcgctggtc catctcccaa tacttcgcta
      721 ttgagaattt tggcaagccc aaaatcaagc actttgacca ccgattcatc actgctgttt
      781 cctgattggc ttccactttg gcttccactg gttggggccg aaggaaataa gtcttcaccc
      841 gtgaccagtc ccatatcctc caaactgacg ccagattgtt tgggtcggct gatcatgatg
      901 ttagagggtt tgagatcacg atgaatcatg ttcagcgcat gggccgcact gacgccgctg
      961 cacacctgtt tcatgatttt gagggtccgc tcgggtgaga ttgggccttc actattgata
     1021 atctcgtgca gaccacgccc ttcgacgtac tccatcacca gataaaacac ccgttgatca
     1081 gtcaccccaa agtcggtcac cccaatcacg ttgagatgat gaatcgctgc tgaggcccgt
     1141 gcttcctgtc gaaaccgttc aattgccgtc ggatcccgac tggcaaaatc aggtaaaacc
     1201 gttttgatgg caaattgacg ccccagatgc acgtgctccg cacgatagac ggtccccatt
     1261 ccgccccgcc ccaggcacga aagaatgcgg tatttcccgt cgatcagggt cgttccaggc
     1321 atactgaacg ctaaatccga ctggtctttg gggcatttga gaacgttatc ctcgtagcaa
     1381 ctgcgacagg ttggacactc cttcatcaat cacactccgg cccactcggc ttttcagtga
     1441 tgttgaaagc ccttttttaa aattgctcgt cgcggaaaga tcccgccgat gcaatggctt
     1501 ttgttgtgta aactcagaat ttccacgatt tgtggagttg tgaagcgtta aggcgggcag
     1561 tttagcacag ggttttcaga tgacgaaacg gcaaatatgg agtgtggcgg cttgaggccg
     1621 ctttatgtgc cgatgaaagc ggcgttaaaa agccaccgca ctcaaaacta tcgctgctcc
     1681 tgaggggact gggatgaagg ctgatcactg gtttcatcct gaaaggcagg aacttcacga
     1741 acaagctgcg gcgccggcag gtaaataaac acaaattcgt catagcgttt atcacggcca
     1801 ttttgacgtt gaaatgcctg cttccgactg gtacttacca caccaacaat tggcaaccgt
     1861 tcttgatcaa ttccaaacgc agattgatct tttccatctg atggttcacc cgtggttgaa
     1921 gtgccaccag atgacgaggt gtcttcgtcc tcgtcctcgt cgtcatcatc ttcatcatct
     1981 gaatcagtgg tctcgtcttc atcgtcctcg tcatcctcat catcctcatc tttgaaatca
     2041 atcggattca ccaaaacccg atagttatct gggattcgtt gacgataata agcggcccac
     2101 tcctgcaaat atcgatttaa cagtggatcc cccacccgca gtgcccgcca ttcacgccca
     2161 ttggccatcg gatctcgcaa ggctgacgga cgtaaaaagt gaaccagtcg cccgttaata
     2221 tcaacacctt tggcaaggtc ttctaatttt gtcacaaaca agccgcctgg attcaactgc
     2281 cccaatcgcc ctgagttgtt gtatcgggca atggcttcgg caatggagtt accgcgctgg
     2341 atcagttcca gttcctgttc ctgttggcgt tcagcctgaa aattcgtgac tgacatcgtc
     2401 a
//
LOCUS       DAOQDO010000894         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39641_length_2399_cov_3.577645, whole genome shotgun sequence.
ACCESSION   DAOQDO010000894 DAOQDO010000000
VERSION     DAOQDO010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39641_length_2399_cov_3.577645"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKZ53_14045"
     CDS             complement(<1..678)
                     /locus_tag="PKZ53_14045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ41610.1"
                     /translation="MKKHLHTLQKRISRLVRPVRLTRLDRRTIKLNPADVACFLVVRN
                     EEERLPYLLNYYREHGIAQFFVVENASTDGTQAFLLTQPDVALWFTDASFRKARCGTD
                     WTLWLLRRYGMGRWCLVVDADELLYYPNSERRSIPDLCTGLEAGGYEAMAAIMLDMYS
                     DRPIHETTYTAGQPFLEVCPFFDRQFYHVKLDRFYNHTHPPSYFGGMRQRVFGSDPLG
                     QYGEHFYT"
     gene            956..>2399
                     /locus_tag="PKZ53_14050"
     CDS             956..>2399
                     /locus_tag="PKZ53_14050"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNJ41611.1"
                     /translation="MEQFSFFEQDTHLTEQTPVQQLAIIGGRLEDTNLDIFNRMLRCA
                     GGKIGVLATASSEPEETGAETVLVFQSYGFEAEYVPIQMNNCQTSAFDPTVIAQLDRL
                     GSVYFTGGDQAHIVSSLIQSGEETPALTKIRELFARGGLIAGSSAGAAVMSDPMIMGG
                     HSLEAVIYGVATEPAQPGLLVGRGLGFFPFGVVDQHFIKRGRLGRLIVAMQAAGCAHG
                     FGVDENTAMFVSNDRLEVVGELGAFIVDQSRAHFSEDGQLLSGIRVSYLDHGDAYDLT
                     HRLVIPNLKKKVREADAGFFKAPAFSRKNIFGSYAIYELMVRLMEGDQSCYASDQGNA
                     YDPASQTEVTVQLQREATTGILTCLEGLDYRYTAIDFSVLVSKRQISPAARRREERAL
                     KYTRKQTLPGELSLNSRLILLGSGLSIEDVDIINEIKRHATTPVGIIATANEDPAQTA
                     REYTRLFKECGLESVFIDLTATNVEAQRHNP"
BASE COUNT          589 a          586 c          643 g          581 t
ORIGIN      
        1 ggtgtaaaaa tgctcaccgt attgccccag aggatcactc ccaaagaccc gctgacgcat
       61 gccgccgaaa taactgggtg ggtgggtgtg gttataaaac cggtcaagtt tgacatggta
      121 aaactggcgg tcaaaaaatg gacagacctc cagaaacggc tgtcctgcag tgtaggtggt
      181 ctcgtgaatg ggtcggtcag aatacatatc aagcatgatg gcggccattg cttcataacc
      241 cccagcctcc agcccggtac agaggtcagg gatactccga cgctctgagt ttgggtagta
      301 cagcaattca tcagcatcca ccaccagaca ccatcgcccc atcccatagc gtctgagcag
      361 ccacagtgtc caatccgttc cacatcgagc tttccgaaac gaggcgtctg taaaccacag
      421 ggcaacgtca ggttgagtca gcaaaaatgc ctgcgtgcca tcagtcgaag cattctcaac
      481 cacaaaaaac tgtgcaatgc catgctcccg gtaatagttg agcaagtagg gaagacgttc
      541 ctcctcgttg cgaaccacca gaaaacaggc gacatctgcc gggttgagtt taatcgtgcg
      601 ccgatcaagt cgggttagcc gtaccggtcg caccaggcgt gaaatgcgtt tctgaagcgt
      661 gtgcaggtgc tttttcattg ggaattcagg cctgggggcg ttgaataggg ctgccaaacc
      721 tctcttttgg attgttctgt gtgttctgtg tgttccatgg tttttttgag aatctagcag
      781 gttggttcga ttgattggac aaccaacagg ttaccaaaga ttgaaccggt agttgaagca
      841 agactgggtg ggtattgtgg gcattgagat aaattttatg tatcactctt tacaaaaaag
      901 aaaatttatg taatatatat tgcatcaatc atcctcccga atagaatatc agcctatgga
      961 gcaattttct tttttcgaac aagatacaca tctgaccgaa caaactccgg tgcagcaact
     1021 ggcaatcatt ggaggtcggt tagaagacac caatcttgac attttcaacc ggatgctgcg
     1081 atgcgctggg gggaaaatcg gggttctggc aacggcttct tctgaaccag aagaaaccgg
     1141 cgccgaaaca gtgctggtgt ttcagagtta tggcttcgaa gccgaatatg tccccattca
     1201 gatgaataat tgccagacct cggcgtttga tccaacggtg attgcccagt tggatcggct
     1261 gggaagtgtg tattttaccg gcggagatca ggcacacatt gtttcatcgc tgattcaaag
     1321 tggggaggaa actccggcgt tgaccaaaat cagggaactc tttgctcgcg gtgggttgat
     1381 tgccgggagc agcgccgggg cggcggtgat gtctgacccg atgattatgg gagggcattc
     1441 gctcgaagcg gtgatctacg gggtggccac cgagcccgct caacccggat tgctggttgg
     1501 tcggggactt ggcttttttc cattcggcgt ggttgatcag cattttatca aacgtggacg
     1561 gctgggccga ttgattgtgg ccatgcaggc ggctggctgt gcgcacggat tcggggtgga
     1621 tgaaaatacc gcgatgtttg tctccaacga ccgacttgaa gtggttgggg aactcggcgc
     1681 ctttatcgta gatcaatccc gcgctcactt ttctgaagat ggtcagttac tttcggggat
     1741 ccgggtcagt taccttgatc atggcgatgc ctatgatctg acccaccgcc tggtaattcc
     1801 caatctgaaa aagaaggttc gcgaagccga cgccgggttt ttcaaggcac ctgctttttc
     1861 gcgtaaaaat atctttggaa gttatgccat ttatgaactg atggtccggt tgatggaagg
     1921 cgaccagtcc tgttatgcca gcgaccaggg caacgcctat gatccagcct cgcaaaccga
     1981 agtgaccgtt cagcttcaac gggaagccac aaccgggatt ctgacctgtc tggaaggtct
     2041 ggattaccgc tatacggcca ttgatttttc agtgctcgtc tcaaaacgcc agatttctcc
     2101 agccgcccga cgacgtgaag aacgggcgct gaaatacacc cgaaaacaga ctctgcctgg
     2161 ggaactgtca ctcaactcgc ggttgatcct gctcggaagc ggcctttcga ttgaagatgt
     2221 tgacatcatc aatgaaatca aacgtcacgc cacaacacca gttggaatta ttgccaccgc
     2281 caatgaagac ccggcccaga ccgcccgcga atatacccga ttgttcaaag aatgtgggtt
     2341 ggaatcggtg ttcattgatt tgactgccac caatgttgaa gcacaacgcc acaatccag
//
LOCUS       DAOQDO010000895         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39648_length_2399_cov_3.076365, whole genome shotgun sequence.
ACCESSION   DAOQDO010000895 DAOQDO010000000
VERSION     DAOQDO010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39648_length_2399_cov_3.076365"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="PKZ53_14055"
     CDS             complement(<1..1516)
                     /locus_tag="PKZ53_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNJ41612.1"
                     /translation="MTRFLFFSFLTLSLMVGVGGITLRHAQANHALTSRFPASQGSQA
                     SEDGLWQVIEESSLNRSQSERQIIPSAYRVVLLNQPVLANLLKQAPLEFSHAAQTTST
                     VMTLPMPDGTFSRFRIEESPVMEPELAAQFPEIKTYCGQGIDDPTAIVRFDRTPAGFH
                     AMVLSAGDTVYIDPYQKGDTETCISYFKRDYQKDANQFRCLIPADNISVQTAESTAGY
                     QRAPFGTTLRTYRLALAATGEYTAFHGGTVAGALAAITTSVNRVNAIYGRELAIRMVL
                     IAQESSIIYTSSGSDPYTNDDGGQMLGQNQANLDRVIGTTNYDIGHVFSTGGGGIASR
                     GGICNSNAKARGVTGLPAPVGDSFDVDFVAHEMGHQFGANHPFNGTTGNCGGGNRAPG
                     SAYEPGSGSTIMAYAGICGGENIQRNSDDYFHGHSMDEIISFITGNGNGCATPSATNN
                     QPPTVEAGNSFTIPRSTPFILTASGNDPDGDAITYCWEQFDLGTQGPPNTDDGSR"
     gene            2066..>2399
                     /locus_tag="PKZ53_14060"
     CDS             2066..>2399
                     /locus_tag="PKZ53_14060"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNJ41613.1"
                     /translation="MLQPDLKEALTFDDVLLLPAQSDVIPTETDTTTFFSRTIKLNIP
                     IVSAAMDTVTESGMAIAMAQQGGIGVVHKNLSISQQYDEVDKVKRSESGMIVDPVTMT
                     PDRPLAEAL"
BASE COUNT          599 a          604 c          576 g          620 t
ORIGIN      
        1 ggcgactgcc atcgtcggta tttggtggcc cctgtgttcc aaggtcaaat tgttcccagc
       61 aataggtaat ggcatcgcca tctggatcat ttccgctggc ggtcaagata aatggtgtac
      121 ttcgtggaat agtaaagcta ttgcctgctt caaccgttgg gggttgattg ttggtggcac
      181 tgggcgtggc gcatccattc ccattgccag taataaaact aatgatttca tccatactat
      241 gaccgtggaa ataatcatcg ctgttacgct ggatattttc gccgccacaa attccggcat
      301 acgccataat ggttgatccg ctaccgggtt cataagctga acctggcgcc cggtttccac
      361 caccacaatt tccggttgtc ccattaaatg gatggttcgc cccaaactga tggcccattt
      421 catgcgccac aaaatccaca tcaaaactgt ctccgacggg tgcgggcaag ccggttaccc
      481 cacgcgcttt ggcgttgctg ttgcagattc cgccacgaga ggcaatgcca ccaccaccag
      541 tactaaacac atggccaata tcataattgg tcgtgccaat cacccgatcc agattggcct
      601 gattttggcc aagcatttgt ccgccgtcat cattggtgta gggatcactt ccggaactgg
      661 tgtaaataat tgaactttcc tgggcaatca gcaccatgcg gatagccagt tcacgaccat
      721 aaatggcatt gacccgattg acgctggtgg tgatggcagc cagcgctcca gcaacggtgc
      781 caccgtggaa agccgtgtat tcgccggttg cggccagcgc cagccgatag gttcgcaagg
      841 ttgtcccaaa tggtgcccgt tgatacccgg cagtactttc agcagtctga actgaaatat
      901 tatctgccgg aatcaaacat cggaactggt tagcgtcctt ctggtaatcc cgcttgaagt
      961 aactgataca ggtttcagta tcgccttttt gataaggatc aatgtagaca gtgtctccgg
     1021 ctgacagcac catggcatga aacccggctg gcgttcgatc aaaccggaca atggccgtcg
     1081 gatcgtcaat tccctgtcca caataggttt tgatctctgg aaactgagca gccagttcgg
     1141 gttccatcac cggtgattct tcgatccgaa accgggagaa agttccatct ggcattggca
     1201 gggtcatcac ggttgaagtg gtttgagcag catgcgaaaa ctcaagcgga gcttgcttga
     1261 gcaaattggc aagtactggc tgattgagca acaccactcg ataggccgaa ggaatgatct
     1321 gacgctcaga ttgagagcga ttcagtgatg attcttcaat gacttgccag agtccatctt
     1381 cagatgcctg agatccctgt gacgctggga aacgagaagt caatgcgtga ttggcctggg
     1441 catggcgaag ggtgattccc ccaacaccca ccataaggct gagggtcagg aaagaaaaga
     1501 aaagaaatcg ggtcatgggt cggtgcttct ttctaaaaaa gagagtcggg ttgcttcaag
     1561 gtgaggcaat tttcagatgt ggtgggagag tttcagttcg atttgaataa aaccagcgac
     1621 cagcattgtt attgacagat gcggttgaga caagtttgtt ctcctgtatt cctgagcttt
     1681 tttcgtgacg attctcaaaa tcaatggagc tgaaagaaaa tctgctctcg tcttttagac
     1741 ctcagaagtg gtgtttttgt tcaaatttca acgatattgt ctgatcccca taaatgactt
     1801 atacatctaa atacttctat taaagggctt tacaactaaa ttcttttttc ctgaaaagca
     1861 acccttgaat ccgggcaaac agccttcgct gtcctggatt gcaagcccac ccgaggattg
     1921 gtacgatttg cccttttatt taccgatgga cttgaggatt catagggtgt ttcacttctt
     1981 caaacccaca accgcttacc tcctgttcaa ccggcgccca gccgtttcac aggacttttc
     2041 cccactcctt gtgcgatcgt gccttatgtt acaaccagat ttgaaagaag ctttgacctt
     2101 tgatgacgtg ctgttgttac ctgcccaaag cgacgtcatt ccaactgaaa ctgacaccac
     2161 cacctttttt tcacgcacta tcaaactgaa tattcccatc gttagcgccg caatggatac
     2221 cgtcactgaa agcggcatgg caattgccat ggctcagcaa ggcggcatcg gcgtcgtcca
     2281 caaaaacctt tccatcagcc agcaatatga cgaagttgat aaagtcaaac gctctgaaag
     2341 tggaatgatc gttgacccgg tgacgatgac tccagatcgt ccactggccg aagctctgc
//
LOCUS       DAOQDO010000896         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39696_length_2398_cov_2.221938, whole genome shotgun sequence.
ACCESSION   DAOQDO010000896 DAOQDO010000000
VERSION     DAOQDO010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39696_length_2398_cov_2.221938"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PKZ53_14065"
     CDS             complement(<1..1170)
                     /locus_tag="PKZ53_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41614.1"
                     /translation="MSNTDKVCICVPVRFAQTATRHLTLLAAMGLLLPWGEVVLSAIP
                     GSGIHQASILAAQQGSTSLRIDLTSSKPKTTSTSLRLNFNNNPVRSSSSTSLRLNINE
                     ISQRLGVQQVGEASAVRERRVPVKPVLTGPQADIAKADTLLDQDKIEEAVALYKKVIT
                     KSPKLVEAHMGLGDALMKQGDFEEAAKKYQEATEIAPTNPDVALGFGVALYRSGRIEE
                     AISQYKLVLEKEKDYAPVYYNLGIAYAHRGEFDEAIKEYQTAITLRKKNYPDAANNLG
                     LIYEAIGNVQGATEQFKAAIAQRPNYPLAHYNLARCYMGQAKFDEALAETKTAVQQQT
                     NFSEAYLTLGNLYLIRSLLRDTNEQELAITAFKQAIQLRENFYPIAHENLAIAYTL"
     gene            complement(1320..>2398)
                     /locus_tag="PKZ53_14070"
     CDS             complement(1320..>2398)
                     /locus_tag="PKZ53_14070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41615.1"
                     /translation="IKVLSPHFANDTSLIKRFQREAKVGWALTHPNIIKVSDFGKTDD
                     GILFMVMEYVEGETMDVYLARSGPLPIGRVLQILEPLCDALSVAHDQNILHRDLKPAN
                     IMISTKKDQEVVKLLDFGIVKLLQPDEQVSALTAVGEVFGTPIYMAPEHLMGLQVGPT
                     ADVYSLGVITHLMLTGQLPHDSEDLRRLFEMKMKELPAPSQKYSFLPAGIDPILQKVL
                     ASHIDKRYQSVNEFFKALKNVADTAPTSHLNKAASASAQSDLLAAMPTIQTPSASSQI
                     PQPTVQSAQTISYATQQREAAAKSGGGGNSTPAAASKENNSPAPASPPADPAAKQSNS
                     TMMIIGAVVVLVIIAVVIFLATKK"
BASE COUNT          498 a          590 c          691 g          619 t
ORIGIN      
        1 caatgtgtag gcaatcgcca ggttttcatg cgcaatcggg tagaaatttt cccggagctg
       61 gatcgcctgt ttaaatgcag tgatggccag ttcctgctcg ttggtgtccc gcagcagtga
      121 gcgaatcagg tacaggttgc ccagtgtgag gtaggcttca gaaaaattgg tttgctgctg
      181 aacagcagtt ttggtttcgg cgagcgcttc gtcaaatttg gcctgcccca tatagcatcg
      241 ggccaggttg tagtgcgcca acgggtagtt tggccgctgg gcaatggccg ccttaaactg
      301 ttcggtggcg ccctgaacgt tgccaatggc ttcataaatc agacccaggt tgttggcagc
      361 atccggatag ttctttttgc ggagcgtgat ggcggtttga tattccttga ttgcttcgtc
      421 aaattcgccc cggtgggcat aggcaatccc caggttgtaa tacaccgggg catagtcttt
      481 ctctttttcc aggaccagtt tgtactggct gatggcttct tcaatacgtc cgcttcggta
      541 gagtgcgacg ccaaacccaa gcgctacatc cgggttggtt ggggcaattt cagtggcttc
      601 ctggtatttt ttggcagctt cttcaaagtc accctgtttc atgagagcat caccgagacc
      661 catatgggct tcgaccagtt tcgggctttt ggtgatgact tttttataca gcgcgacggc
      721 ttcttcaatt ttgtcttgat cgagcaaggt atccgctttg gcaatgtcag cttgcgggcc
      781 ggtcaggacc ggtttgaccg gaacacggcg ctcacgcacg gcactggctt caccaacctg
      841 ttgtacccca aggcgctggc tgatttcatt gatgttgagc cgcagggaag tcgaagaact
      901 tgaccggacc gggttgttgt tgaaattcag tcgcagtgag gtggaggtcg tttttggctt
      961 tgaggaagtg aggtcaatcc gcagtgacgt cgagccttgc tgggcagcca ggatgctggc
     1021 ctggtggatg cctgatcctg gaatcgcact gagcaccact tcaccccagg gcaaaagcag
     1081 acccatggct gccagcaatg tcaggtgtcg agtggccgtc tgggcaaaac ggaccggaac
     1141 gcaaatgcaa accttgtcag tattcgacat agaacttttc ggatatggtc gagagttccg
     1201 ccttgtggcg gggagttata gaaattatgg aagataattg ccttcggtga agaagctgtt
     1261 tgaagccaga actctgaact tttcctgaaa tccgatgccg cgcgtttcca cgcttgagtc
     1321 tattttttgg tggccaaaaa aatcaccacg gcaattatca cgagaacaac aactgcgcca
     1381 atgatcatca tggtggaatt ggactgtttg gcggctgggt cagccggggg tgatgctggc
     1441 gccggagaat tgttttcctt cgacgcagcg gctggcgtcg agttgcctcc tccccctgat
     1501 ttagccgctg cttctcgttg ctgagtcgca tacgagatgg tttgggcact ttggacggtg
     1561 ggttgcggta tctgcgatga cgcgcttggc gtttgaattg tgggcatggc cgccagcaag
     1621 tcagactggg ctgaagctga ggctgcctta ttcagatggg acgttggtgc cgtatcagcc
     1681 acatttttca gggctttgaa aaattcgttg acgctctgat accgcttatc aatgtgcgag
     1741 gccagcactt tctgcaaaat cgggtcaatt ccggcaggca ggaacgaata cttctgcgat
     1801 ggtgccggca actctttcat tttcatttca aagagtcggc gcaggtcttc gctgtcgtgt
     1861 gggagctggc cggtgagcat cagatgggta atgacgccca ggctgtacac gtcggcggtg
     1921 gggccgacct gcaggcccat taaatgctct ggtgccatat agatcggcgt cccaaacact
     1981 tcaccgacgg cagtcaaggc tgaaacctgc tcatctggct ggagcaactt cacaatgcca
     2041 aaatcaagca acttgaccac ttcctggtct tttttggtgc tgatcatgat attggccggt
     2101 ttgaggtcac gatggaggat gttctgatcg tgcgccactg acaaggcatc gcagagcggt
     2161 tccagaatct gcagcacgcg tccaatcggc aatggcccgg agcgggccag atagacatcc
     2221 atggtctcac cttcgacata ctccatgacc ataaacagga tgccgtcatc ggttttgccg
     2281 aaatcgctga ccttgatgat gttggggtgg gtcaacgccc acccaacctt ggcctcacgc
     2341 tgaaagcgtt tgatcaggct ggtgtcattg gcaaagtgtg gcgacagcac tttaatcg
//
LOCUS       DAOQDO010000897         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39728_length_2397_cov_2.404355, whole genome shotgun sequence.
ACCESSION   DAOQDO010000897 DAOQDO010000000
VERSION     DAOQDO010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39728_length_2397_cov_2.404355"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PKZ53_14075"
     CDS             complement(<1..1539)
                     /locus_tag="PKZ53_14075"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNJ41616.1"
                     /translation="MNFETVRVPDFVRFVCRCLTVGAVGLLMFLAAPLRYDYHAQQPT
                     LQNVSISRELVERATILRQTFANNPKAAHTETEYLDLIGIYQRAVRLDTEQTVGDAAL
                     IGQAEVFREMGSRFQKTRYFYSAIGCYQNLLETYETSPHTTRALISIAQIYEHNLRET
                     NQAARAYQLIVEQYPSSVSAREAMASLARIGTVSGGQATGENVLFAASQDDQAGITTI
                     GQIRHFSGPDYARVILDLSIPATYERSLNGSTLVLRLPSAKVSPLLAGRPIAITTNGL
                     LKNIRYSTSTSAVEVAIECTQLRDFAIFALDDPPRLVVDLRGSRSIAEDAEILASTTA
                     LPSVKGAQQTAAGDITLMRALGLKVKRIVIDPGHGGSDFGATGKDNIYEKDVVLDIAL
                     RLRAAIQQRMKDVEVILTRDSDRFIPLEERTAIANSRQADLFISIHTNSSPSSLASGV
                     ETYFLSRDASKEELEVATRENATTSRNVNDLLDLLQQIALNNKVAESRDFAQHVQTNL
                     VNGLT"
     gene            1913..>2397
                     /locus_tag="PKZ53_14080"
     CDS             1913..>2397
                     /locus_tag="PKZ53_14080"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNJ41617.1"
                     /translation="MRLKLLASVLLTIVLLGQALPLRADEGMWLPDTIGNRQLLQKMK
                     AKGFALRPEDIFSNTAPSLKDAIVIVDGGTAEFVSDRGLLLTNHHVAFQAIATESTPE
                     KDYLKNGFVSKSETEEIAIKDETYHCKILKESRDVTTEVLAGVTDQMNAEERNTAIET
                     KR"
BASE COUNT          604 a          583 c          656 g          554 t
ORIGIN      
        1 ggttaatcca ttaaccagat tggtctgaac gtgttgggca aaatcacgtg attcagccac
       61 tttgttgttg agggcgattt gttggagcaa atcaagcagg tcgttgacat tgcgcgaggt
      121 ggtcgcgttt tcacgggtgg cgacttcgag ttcttccttg gaggcatccc gactcaggaa
      181 ataggtctca acgccactgg ccagactgga tgggctggaa ttggtgtgga ttgaaataaa
      241 caggtccgcc tgccgggaat tggcaattgc ggttcgttct tcaagtggaa taaaccggtc
      301 actatcgcgg gtcaggatga cctcaacatc tttcattcgt tgttgaattg ctgctcgaag
      361 tcgtaaggca atgtcgagaa ccacatcttt ttcatagatg ttgtctttgc cggtggcgcc
      421 aaaatcagag ccaccgtgac cggggtcaat cacgatccgt tttactttga gtccgagggc
      481 ccgcatcagg gtaatatccc cagccgccgt ttgctgggcg cctttgactg acggaagagc
      541 cgtcgtggac gccagaatct cagcatcttc agcaattgag cgtgacccgc gcaggtcaac
      601 caccagtcgt ggcggatcgt cgagggcaaa aatggcaaag tcacgaagct gggtgcactc
      661 aatggcaact tcgaccgcgc tggttgaggt ggaatagcgg atatttttga gaaggccatt
      721 ggtcgtgatt gcaattgggc gaccggcaag aagcggggaa actttggctg aggggagtct
      781 cagaaccagc gtggagccgt ttaacgaccg ctcataggtg gcagggatac tgagatcgag
      841 aatgacccgg gcataatctg gccccgaaaa gtggcgaatc tgcccaatgg tggtgattcc
      901 ggcctgatca tcctgggaag cggcaaaaag tacattctcg ccagtcgcct gaccacctga
      961 cacggtgcca attcgggcga ggctggccat cgcttcgcgg gcactcactg aactgggata
     1021 ttgctcaacg atcaactggt aggcccgtgc cgcctgattg gtttcccgca ggttatgttc
     1081 gtaaatctgg gcaatactga tcagggcacg agtggtatgt gggctcgttt cataggtttc
     1141 cagcaggttc tgatagcacc cgatagcact gtagaaatac cgcgtttttt gaaaacgcga
     1201 ccccatctca cgaaaaacct cagcctgacc aatcagggcg gcatcgccga cggtttgttc
     1261 cgtatcaagg cggactgcgc gctgataaat gccgatcagg tcaagatatt cagtttcagt
     1321 gtgtgcagct ttgggattat tggcgaaggt ctgccgcaag atcgtggcgc gttcaaccag
     1381 ttcgcgggaa atggagacat tttgcagcgt tggttgttgg gcgtgatagt catagcgcaa
     1441 tggagcggcc aaaaacatca gcaacccaac cgcacccacc gtcaaacacc gacagacgaa
     1501 ccgcacgaaa tctggcacac gtacggtttc aaagttcatt cgaagcatac tgctacaggg
     1561 tttcgctctt aaattttcag cgcaaaaggt agctgaatga aacggggagg agccttcccc
     1621 acaaagcttc aggtgatgaa gcagaaactg cgcttaaccc cttgattgat ttcggaaaag
     1681 atgcccttga taggttagga agacaggcgc tattgtacca cagcttgggg ttctgccaag
     1741 cgaattcatc cgtcaccaat tgccagaaac ctgtcatttt ccaatctttc cgagtgccaa
     1801 acatttatcc acaggtgaca gctccccggt cagacatcga tctgaatcac tggcaattgc
     1861 tcacactttt tcaaaagagt atcccttacc aggagggaaa caaggagaac gaatgcgatt
     1921 aaaactattg gcctctgttt tacttacgat tgtgttgttg gggcaggctt tgccgctccg
     1981 tgctgacgaa gggatgtggc ttcctgatac gattggaaac cggcaactcc ttcaaaagat
     2041 gaaagccaaa ggctttgcct tgcgcccgga agacatcttc tcaaacaccg caccaagctt
     2101 aaaagacgcg atagtgattg ttgatggcgg tacggctgag tttgtctcag accgggggtt
     2161 actgctgacc aatcaccacg ttgctttcca ggcgattgca actgagagca ctccggaaaa
     2221 ggactacctg aaaaatggat ttgtttccaa aagcgagacg gaagaaattg caatcaagga
     2281 tgaaacctat cactgcaaaa tcttgaaaga gtcccgtgac gtgacgactg aggtgctggc
     2341 cggtgtcact gaccagatga atgccgaaga gcgcaacacg gccattgaaa ccaaacg
//
LOCUS       DAOQDO010000898         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39747_length_2396_cov_3.353268, whole genome shotgun sequence.
ACCESSION   DAOQDO010000898 DAOQDO010000000
VERSION     DAOQDO010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39747_length_2396_cov_3.353268"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..>2396
                     /locus_tag="PKZ53_14085"
     CDS             147..>2396
                     /locus_tag="PKZ53_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ41618.1"
                     /translation="MTVRPRAAFTQACAFLTCICLAGTPLTALGAPLIQAASSGKSAT
                     AQKNADKPGKVAGLVVNVATGDPIPNAGVEIVSTQQTFYTDNDGNFVLTLPPGSYEVR
                     VFKSGFVDTKKPVTIVAGDVFPLDFALSTTGSGEVVEVNATSGSTEIAMVEERKTAST
                     IVDSLSLREINNDVAGDAAGVLQRAPGLSIVDNKFVYVRGLGDRYSNTVMNDAVMPTP
                     QAERKVVPLDQVPSDLIQNLKILKTFTPDQPGEFAGGLVKIETLEFPNRSSLKFSTSF
                     GGNTQTTFQDMLTSPGDRLDFLGFGLGRRELPSIIPNKPLVRGSDLLPGFTPQELQTF
                     GRAFENVWEPRKSTAPINQSYSLAGSTQIGKLGFIGTLTYGNSSTVTPEIQRYPRVST
                     GPDGTPVVSAQNLYDYVSGVNSVRLGLLGNAAYKLNDKNKFLFKNFFSNDSRDEAREF
                     QGYFDDRTTDILNRRLRYTRTRTETHQLAGDHLISKLGDSIITWRFTYSRALLDEPDL
                     RETLYDFNSSLGKFVFFQGGQSGFRMFTKMRENIREPAIDWSKFVFKSNITFNFKAGF
                     QFTNRDRSFNARRLRFTARSLAGIDTSLSPENLFEASNINPEGFEVQENTRPTDFFVA
                     KQDIIAGYGMADVTYNKFRLIFGARVENSEQQVQTFELFSSNPEPTRATLENTDVLPS
                     IGFVYNIKPNINLRIGYSQTVTRPQFRELSPFEFTEATGGRSTLGNPNLVRTKIQNYD
                     ARFEMFQSSG"
BASE COUNT          603 a          670 c          569 g          554 t
ORIGIN      
        1 ttcaacctga ttcacaatcc tgcattgatt cgattcaaac ctgaaggcgt gacactccac
       61 tcagtcagtc acagcctttg actcaaccga gtccgaagac tttttggtgt gttttctctt
      121 ttttcaaatc tcatgaggta caatgtatga cagttcgtcc acgtgcagcc ttcactcagg
      181 cttgcgcctt cctgacgtgt atctgtctgg cgggaacgcc attaacagca ctcggtgcgc
      241 cgctcattca agccgcatca tcaggaaaat cggcaactgc ccaaaagaac gcagataaac
      301 ccggcaaagt ggccggactg gtcgtgaatg tggcaacggg tgacccgatt ccaaatgccg
      361 gagttgaaat cgtttcgacg cagcagactt tctacacgga caatgacggc aattttgtcc
      421 tgacactccc acccggaagc tacgaagtcc gcgttttcaa gtcgggtttt gttgacacca
      481 aaaaaccagt caccatcgtg gcgggagatg tgtttccgct tgattttgcg ctttcaacga
      541 ccggaagcgg cgaagtggtc gaagttaacg ccaccagcgg cagcactgaa attgcgatgg
      601 tcgaagaacg caaaaccgcc agcacgattg ttgattcgct ctcattgcgt gaaatcaaca
      661 acgacgtcgc cggagacgcc gcgggggtgt tgcaacgcgc accgggactt tcaattgttg
      721 acaacaaatt tgtctatgtt cggggactgg gagatcgcta tagcaacacc gtcatgaatg
      781 atgcggtgat gccgacgccc caggccgaac gcaaagtggt accgctcgac caggtgccgt
      841 ctgatttgat tcagaactta aagattttaa agacctttac cccggatcaa ccgggtgaat
      901 ttgccggcgg gctggtcaaa attgaaacgc ttgagtttcc caaccgctcg tcactcaaat
      961 tctcaacctc gtttggcggc aacacccaga ccacgttcca ggatatgctg acgtcacctg
     1021 gcgaccgact cgatttcctg ggttttggat tgggacggcg tgaattgcca tcaattattc
     1081 caaacaagcc gctggtgcgt ggcagcgacc tgctccctgg ctttacaccc caggaattac
     1141 agacctttgg tcgcgcattt gaaaatgtct gggaaccacg taaatcaacc gcaccgatca
     1201 atcaaagtta tagcctcgct ggcagcacac aaatcggaaa gctcggtttt atcggcaccc
     1261 tcacctatgg caacagttca acggtgactc cagaaatcca gcgctatccg cgtgtgtcaa
     1321 ccggtccaga tggcaccccg gtggtcagcg cccaaaacct gtatgactat gtgtcaggcg
     1381 tcaacagcgt tcggcttggg ttgcttggca atgccgccta caaactcaat gacaagaaca
     1441 aattcctgtt caagaacttc ttcagtaacg attcgcgtga cgaagcccgc gagtttcaag
     1501 gctattttga cgaccggaca accgacattc tcaatcgccg tctgcgctat acccgcaccc
     1561 gaaccgaaac ccaccaactg gctggggatc acctaatcag caaactgggg gatagcatca
     1621 ttacgtggcg ctttacctac tctcgggcgc tgcttgacga gccggatttg cgcgaaacgc
     1681 tctatgactt taactccagc ctgggcaagt ttgtgttctt ccagggtgga cagagcggct
     1741 tccgaatgtt taccaaaatg cgtgaaaaca tccgcgaacc agcgattgac tggtcaaagt
     1801 ttgtctttaa atcaaacatc acgtttaact tcaaagccgg cttccagttc accaaccgcg
     1861 accgatcctt caatgcccgc cgcttgcggt ttacggcccg cagcctggct ggaattgaca
     1921 catcgctctc tcctgaaaat ctgtttgaag cctcaaacat caaccctgaa ggatttgaag
     1981 tccaggaaaa cacccgccca accgactttt tcgtggccaa acaggacatc attgccgggt
     2041 atggcatggc agatgtcacc tataacaaat tccggttgat ttttggggca cgggttgaaa
     2101 actcagaaca gcaagttcag acctttgaac tgttttcatc aaaccccgaa ccaacccggg
     2161 ccacgctgga aaataccgat gtcctgccca gcatcgggtt tgtctataac atcaagccaa
     2221 atatcaatct gcgaattggc tacagccaga ctgtcacccg tccacaattc cgcgaactca
     2281 gcccgtttga atttaccgaa gctactggcg gtcgctcgac cctcgggaac ccaaatctgg
     2341 tgcggaccaa aattcaaaat tatgacgccc ggtttgaaat gtttcagtca tcgggg
//
LOCUS       DAOQDO010000899         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39792_length_2394_cov_3.806327, whole genome shotgun sequence.
ACCESSION   DAOQDO010000899 DAOQDO010000000
VERSION     DAOQDO010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39792_length_2394_cov_3.806327"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..>2394)
                     /locus_tag="PKZ53_14090"
     CDS             complement(134..>2394)
                     /locus_tag="PKZ53_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNJ41619.1"
                     /translation="LDRAGYLNPEKTGDANRGKYIVKGDTLEITPSKDSAQGAWAFSP
                     LRLRFAGESIKDIYALDTKQSLTECFLEPELLTSLNQNKEKRTIIEYKDIPKNLKNAI
                     VAVEDRNFEKHRGIDFRGLARAIYHNFTTEDGTVQGASTITQQLVKNYILTPERTLKR
                     KLEEAFISILLEVKLSKEQIMQMYCNQIYLGQDGNYSINGVGEAARFYFNKDLTDLNL
                     QECALMAGIIRGPGYYSPFQHRERAKERRLKVLNDMLEETYITQAQFDAVKDIDLGVK
                     SRTVSSNADAPYFLDFLQQKLAEHGKTDVLKAQSVRIYSSIDMELQRLADKAFRDGID
                     RLLKTNKGFGPKADQLQGALVAVNPKTGEVLAMVGGRDYSKTQLNRATEAHRQPGSVF
                     KPIVYTTAMMTGEGLPCCSEISNAITPSTLFMDAKQQFLYEDRVYEPDNFGGSYSNKE
                     ITVREALTKSKNTITVRIAECVGYDKVARTAEKLGLPMPREKKPDGSTKLKSFPSVAL
                     GVTEATPLEVARAYTAFPNLGMWVDPAPFTTLIDTRGQLEKITPKRERGISAPVASVM
                     THLLEGPIARGTAVKAQAVGAKALAGKTGTSRDAWFAGFTPNLVCVVYVGFDDNSQLG
                     ITGGEAALPIWVDFMKGALALRPDLGGEAFPDTPGVEKVKIDLDSGKLATEECPNAID
                     GYYIAGTIPEEYCDHNGLPSLLPEGILPGDEDAIPPRPEEPNQPATNRKPFPGWRKLP
                     EVIGQAVRRGRG"
BASE COUNT          552 a          620 c          566 g          656 t
ORIGIN      
        1 taggaatcaa taccttccaa gaaccaagaa ccaagaacca agaaccaaga accaaaaacc
       61 actttcttca ttcttcattc tcaattcttc attctcaatt cttcattctc aattcttcat
      121 tctcaattct tcatcagcct ctgccccggc gcacggcctg accaatgact tcaggaagtt
      181 tgcgccagcc aggaaatggt tttcggttgg tagctggttg atttggctcc tcaggtcgag
      241 gtggaatggc gtcttcgtca ccagggagga ttccttccgg aagtaaagac ggaagcccat
      301 tgtggtcaca gtactcttcc gggatcgtgc cggcaatata atagccgtca atggcatttg
      361 gacattcttc ggtagccagt tttcctgaat ccaggtcaat tttgaccttt tcaactccgg
      421 gagtgtccgg gaaagcctca ccacccaggt caggtcgtaa ggccagagca cctttcataa
      481 aatcaaccca gatcgggagt gcggcttcac cacctgtgat gcctagctga ctgttatcgt
      541 caaatccaac atagaccaca cagaccaggt ttggtgtaaa cccggcaaac caggcatccc
      601 ggctggtgcc agtcttgccg gccagggctt tggcaccgac ggcctgcgct ttgacagccg
      661 tgccacgggc aattgggcct tcaagcaagt gagtcatgac cgaggccacc ggagctgaga
      721 ttccccgttc gcgtttcggt gtaatttttt ccaattgccc cctggtatca atcagcgtgg
      781 tgaacggcgc cggatccacc cacattccca ggtttggaaa agccgtgtag gctcgtgcga
      841 cttcaagcgg tgtggcttca gtgacaccta aagcaactga aggaaatgat ttcagtttgg
      901 tcgaaccatc cggctttttc tcgcgtggca tcggcaaccc aagcttttcg gcagttcgag
      961 ccactttgtc atatccgaca cattcagcaa tgcgcacggt gatggtgttt ttcgacttgg
     1021 tgagcgcttc ccgaaccgta atttccttat tgctatagct ccccccaaaa ttgtctggtt
     1081 catagacgcg atcttcatac agaaactgct gtttcgcatc cataaacaaa gttgacgggg
     1141 taatcgcatt tgaaatttca gaacaacagg gtaaaccttc cccggtcatc atggcggttg
     1201 tatagacaat tggtttaaag actgaacctg gctgacgatg agcttcggtc gcccgattta
     1261 actgggtttt gctgtaatca cgaccaccaa ccatcgccaa cacttccccg gtttttgggt
     1321 tcacggccac cagtgctccc tgaagctggt ccgcctttgg tccaaacccc ttgttggttt
     1381 tgaggagtcg gtcaattcca tcacgaaatg ccttgtcagc caggcgctgc aattccatat
     1441 caatgctgct gtaaatgcgt acggactggg cttttaagac atctgttttg ccatgttccg
     1501 ctaatttttg ctgaaggaaa tccaggaaat agggcgcatc ggcgttgctg ctgacagtcc
     1561 gcgatttcac acctaaatca atgtctttga cggcatcaaa ttgagcctgg gtgatatagg
     1621 tttcttcaag catgtcgttg agcactttga gccgtcgttc tttggctcgc tcgcggtgtt
     1681 gaaacggtga ataatacccg ggtccacgga taatcccggc catcaaggcg cattcctgta
     1741 agttcaggtc tgtcaaatct ttattgaaat aaaaccgggc agcttcccca acaccattga
     1801 tcgaatagtt cccgtcctgc cccagataaa tctggttgca gtacatctgc ataatctgct
     1861 ctttcgacaa tttgacttcg agcagaattg aaataaaggc ttcttcgagc ttgcgcttca
     1921 aggtgcgctc aggtgtcaaa atgtagtttt tgaccagttg ttgggtaatg gtcgaagctc
     1981 cctgaactgt tccatcttcg gttgtgaagt tgtggtaaat cgcccgcgcc agtccgcgaa
     2041 aatcaatccc ccggtgcttt tcaaagttgc gatcttcgac ggcaacaatg gcatttttca
     2101 gattcttggg gatgtctttg tattcaatga tagttcgctt ttccttgttc tgattcagcg
     2161 atgtcagcag ttccggctcc aggaaacatt cggtcaaact ctgtttggta tccagggcat
     2221 agatatcttt gatgctttcg ccagcaaacc gaagtcgaag gggtgaaaaa gcccaggcac
     2281 cttgagcgga atccttactc ggggttatct ccagggtatc accttttaca atatattttc
     2341 cgcgattggc gtcgcctgtt ttttcagggt tgaggtaccc ggcacgatcc agat
//
LOCUS       DAOQDO010000900         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39810_length_2394_cov_2.139376, whole genome shotgun sequence.
ACCESSION   DAOQDO010000900 DAOQDO010000000
VERSION     DAOQDO010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39810_length_2394_cov_2.139376"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PKZ53_14095"
     CDS             <1..772
                     /locus_tag="PKZ53_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ41620.1"
                     /translation="TTRQSELKLPDTVLFDRLARGNFTAMLAVLDQGKPVQAEINRRT
                     VSVTSSFSLGATFNTDGTLIGNETAFLHLFPNRDSGSVTFGLVTIAPTADRQRVVADL
                     KRKFPDDVNVLTREEFIQDARDFQLRYTPIGFIFGLGTVMGFLVGLVMVYQILSSDVA
                     DHMSEYATFKAMGYTDLYLLGIMIEESLILAGLGFIPGLISGFGIMRLIHAGTALPVS
                     FPMTRIAGVFLLTLGMCIVSGFIAARRIRQADPAEIFS"
     gene            769..1521
                     /locus_tag="PKZ53_14100"
     CDS             769..1521
                     /locus_tag="PKZ53_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ41621.1"
                     /translation="MTVSACPLTPVVVLRGVNHFFGTDTLQRQILFDIDLDLQAGEIV
                     ILTGPSGSGKTTLLTLVGALRTVQSGSAQVFGTELVKAAWKDLFETRRKLGFIFQAHN
                     LHRSLTALQNVMMGLEVHAGISREERLLRSRIMLKEVGLLDHAGKVPAQLSGGQRQRV
                     AIARALVSHPKMILADEPTAALDKHSGREVVDLLRRLAIEQGTTILLVTHDNRILDIA
                     DRILAMEDGRLIGVESPKFQRLAGEEVINNVV"
     gene            complement(1468..>2394)
                     /locus_tag="PKZ53_14105"
     CDS             complement(1468..>2394)
                     /locus_tag="PKZ53_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41622.1"
                     /translation="GEANLELYVIDKEEVYLSVPQAHSSPEALQVILNRILVKPKDVF
                     MANEINGEWNIGPYTLTKTPEKAAFFRTQAGNIKIDHAAVFQFPFKQATYTLNLKEMS
                     DFIGNHVIFGGPLRADTDIRDGDKHLIFFNHGAFVSRGTEPSVKRLVADLTKDIPATD
                     PDVREKKIQRLLDFVAGEIEYDGNEATRNVETLKRPNEVLMSRRADCSNKTILLGSML
                     EQLGEDYLFLYCPKHITVGVRQGNFPTTNGLTLEWEGKTWTIAESTAVGFQIGKDRLK
                     DPALVKQVRYVQRPHSNDIIYNFFTSETLEFR"
BASE COUNT          520 a          583 c          652 g          639 t
ORIGIN      
        1 gacgacccgg caatcagagt taaagcttcc ggatacagtt ctgtttgacc ggctggcacg
       61 agggaatttc accgcgatgc tggcagtact tgatcaaggc aagcctgtcc aagctgaaat
      121 caaccgccga acagtgagtg tgaccagctc attttcactt ggtgcgacct ttaacactga
      181 tggcacgctg attggaaatg agacagcgtt tctgcatctt tttccgaatc gggattcagg
      241 ttccgtcaca tttggactgg tgaccatcgc acccacggct gaccgtcaac gggttgtggc
      301 tgacctcaaa cgaaagtttc cagatgacgt caacgtgttg acccgtgagg agttcatcca
      361 ggacgcccgt gattttcaac tccgatatac accgattggg ttcattttcg gtctgggtac
      421 cgtgatggga tttctggttg gtctggtcat ggtctatcag attttgtcat ctgatgtggc
      481 tgatcatatg agtgaatatg cgacctttaa ggcgatgggg tacaccgacc tctatttgct
      541 gggaatcatg attgaggaat cgctcatcct ggccggtctc gggtttatcc cgggtctgat
      601 ttccggtttt ggcattatgc gcctgatcca cgctggcaca gcgttgccag tgtcgtttcc
      661 gatgacacgc atcgctggcg tttttttact gacgctggga atgtgtattg tttccgggtt
      721 tattgctgcc cgacgcattc ggcaggctga cccggcggag attttttcat gacagtttcg
      781 gcctgccccc tgactccggt ggttgtgttg cgtggggtaa accatttttt cggtacagac
      841 acgctgcagc ggcaaattct cttcgacatt gatctggatt tacaggccgg agaaatcgtc
      901 atcctgaccg ggccatctgg atccgggaaa acgacgctcc tgacccttgt cggtgcactg
      961 cgaaccgttc aaagcggaag tgcccaggtc tttggcaccg aactggttaa ggcagcctgg
     1021 aaagacctgt ttgaaacccg tcgtaaactg gggtttattt ttcaggccca taacctgcat
     1081 cgcagtctca ccgcgttgca aaatgtcatg atggggctgg aggttcacgc cgggatttcg
     1141 cgcgaggaac ggctgctcag atcccgcatc atgttgaaag aagtcggctt gctcgaccat
     1201 gccgggaagg tcccagctca actctccggc ggtcaacgac agcgggttgc gattgcccgg
     1261 gcgctggtca gtcatccaaa aatgattctg gctgacgaac ccacagcggc gcttgataaa
     1321 cattcggggc gcgaagtcgt ggatctgctt agacgtctgg cgattgaaca gggcaccacc
     1381 attttgcttg ttactcacga caatcggatc cttgatattg cggaccggat tttggcgatg
     1441 gaagatggac ggttgatcgg tgttgaatca ccgaaattcc agcgtctcgc tggtgaagaa
     1501 gttataaata atgtcgtttg agtggggccg ctgaacatac ctgacctgct tgaccagggc
     1561 tggatctttg agccggtctt tcccaatttg aaacccaacc gcagtggatt cagcaatggt
     1621 ccaggttttt ccttcccact ccagggtgag gccattggtc gtcggaaaat tcccctgtcg
     1681 gacaccgacg gtgatgtgtt tcgggcagta caaaaacagg tagtcttcgc cgagttgttc
     1741 aagcatcgag ccaagcaaaa tggttttgtt gctgcaatca gcgcgccggc tcatcaggac
     1801 ttcgttgggg cgtttgaggg tttcgacgtt gcgggtcgct tcgttgccat catattcaat
     1861 ttcaccggcg acaaaatcga gcaaccgctg gatcttcttt tcacggacat cgggatccgt
     1921 tgctggaatg tctttggtta aatcagcaac cagtcgtttg acggatggtt cggtaccacg
     1981 tgatacaaaa gcgccgtggt taaagaaaat cagatgtttg tcgccatcgc ggatatcggt
     2041 atcagcccgc aatggaccgc caaaaatgac gtgattccca ataaaatcag acatttcctt
     2101 gagattcagg gtgtaggtgg cctgtttgaa tggaaattgg aacaccgccg catggtcaat
     2161 tttgatattg ccggcctggg ttctgaaaaa tgctgctttt tccggcgttt tggtcaaggt
     2221 ataggggcca atattccatt ccccgttgat ttcattggcc ataaaaacgt cttttggctt
     2281 gaccagaatc cgattcagaa tgacctggag tgcttcaggc gagctatggg cttgcggaac
     2341 tgagagatat acctcttctt tatcaatgac atagagttcc agattggctt cacc
//
LOCUS       DAOQDO010000901         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39812_length_2394_cov_2.041898, whole genome shotgun sequence.
ACCESSION   DAOQDO010000901 DAOQDO010000000
VERSION     DAOQDO010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39812_length_2394_cov_2.041898"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKZ53_14110"
     CDS             <1..1006
                     /locus_tag="PKZ53_14110"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNJ41623.1"
                     /translation="HLGRPKGTPQPKYSLQPVIEPLSQLLGIPVTFAADCIGDEAKAK
                     AEALQPGEVLLLENLRFYKEEEKNNPDFARSLAELAEVYVNDAFGTAHRAHASTAGIT
                     EFLTHKAAGLLMEKELTYLGKALHDPEHPFVAILGGAKVSDKIEVIDNLLKTANVMLI
                     GGAMSYTFLKARGLEIGKSLVEDDKLDLARELEQKAKERGVKFLIPTDHLVADLSRPD
                     LAPETVSVAETPADLAGYDIGPATVAAYTEEIAKAKTIVWNGPMGIFENPLYAEGTMA
                     VAKAVAACDGTTIIGGGDSVAAINQAGLADKVSHVSTGGGASLEFLSGLELPGVAVLK
                     "
     gene            1213..>2394
                     /locus_tag="PKZ53_14115"
     CDS             1213..>2394
                     /locus_tag="PKZ53_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ41624.1"
                     /translation="MSQIFLPASFIGAGGDQVLFELFIIFAAAKIAAELFERFKQPAV
                     VGEILAGVVIGPSVLKLVAPSQVTQALSEIGVMLLLFMVGLEINSSALLKVGGRALLV
                     AVSGVVVPFIAGWAVFSLWQTTGYQSIFMGAALVATSVGITARVLGTMNVLNTKASQI
                     ILGAAVIDDILGLIVLSIVSSLARGGINYAELGVTAGLSIGFTIFVMVVGTRLVKRAQ
                     PQIENLKVSEAYFIVGILLCLGLSWLGTLVGVAALIGAFLAGMALGESTEHTNLHAQA
                     SSVMEFSVPFFLVSIGLQLDLSVFKDAQVIALALVTTLVAVATKLVACGLVALPMGKR
                     TALQVGMGMVPRGEVGIIVAQIGLKSQVLTPALYAVVVFMAVATTLVAPPFLAALFQP
                     SP"
BASE COUNT          541 a          538 c          664 g          651 t
ORIGIN      
        1 acatctgggt cgtccaaagg gaacacccca gccgaaatac agtttgcagc cggtcattga
       61 accattgtca caattgcttg gcattccagt tacttttgcg gcggattgta ttggcgacga
      121 agccaaagca aaagctgaag ccctccagcc aggtgaagtt cttctgcttg aaaacctccg
      181 tttttacaaa gaagaagaga aaaacaaccc tgattttgcc cgatcactgg ctgaattggc
      241 cgaggtctac gtcaatgacg catttgggac tgcacaccgg gcgcacgctt cgacggctgg
      301 cattaccgag ttcttgactc acaaagctgc tggtctgttg atggaaaaag aattgaccta
      361 tctcgggaaa gccctccatg acccggaaca tccgtttgtg gccattctgg gtggtgccaa
      421 agtctctgat aaaatcgagg tcattgacaa cttgttgaag accgccaatg tcatgttgat
      481 tggtggggca atgtcctaca cttttttaaa agcccgtggg ttagaaattg gaaaatcact
      541 ggttgaagat gacaaactcg acctggcccg cgaacttgaa caaaaagcca aagaacgcgg
      601 ggtcaaattc ctgatcccaa ccgatcatct ggtggctgat ttgagtcgcc cagacctggc
      661 cccagaaacg gtttctgtgg ctgaaactcc ggctgatctg gctggatatg acattgggcc
      721 ggcgacagtt gcggcctaca ccgaagaaat tgcaaaggcc aaaacgattg tctggaatgg
      781 ccccatgggt atttttgaaa acccgctcta tgccgaagga accatggcgg ttgccaaagc
      841 cgtggctgcg tgtgacggaa ccacgattat tgggggcggt gatagcgtgg cggcaatcaa
      901 tcaagccggg ttagctgata aggtctcgca cgtttcaacc gggggtggag cgagcctgga
      961 attcttatct ggactggaac ttcctggtgt ggcggttttg aaataacttg taccagttcg
     1021 tagtcagttg tccgtagtca gtggttcaat aagcttattt aattgaacta tttgactatt
     1081 ttccaatacg aggacttctg tacaaattgg tatgatcctc atcaaaaggt acaaaaatga
     1141 agggaaggca ttgcttcttt tttgtacctt ttgcattttc caaagctgtg tatcgggaaa
     1201 gattccatgc tgatgtcaca gatttttctt ccagccagtt tcattggagc cggcggcgat
     1261 caggttttat ttgaactgtt tatcattttt gccgcggcca aaattgcggc tgaattgttt
     1321 gaacggttca aacagccagc cgttgtcggt gaaattctgg ctggggttgt cattggccca
     1381 agtgtactca aactggtggc tccctcacag gtcactcagg cactatctga aattggcgtg
     1441 atgctgctcc tgttcatggt tgggttggaa attaactcca gcgccctttt gaaagtgggg
     1501 ggccgcgctt tgctggtggc cgtttcagga gttgtggtgc cattcatcgc cgggtgggcc
     1561 gtttttagcc tgtggcagac aaccgggtac caaagtattt ttatgggggc tgccctggtg
     1621 gcaaccagcg tggggattac ggcccgggtg ttgggaacga tgaatgtgct caataccaaa
     1681 gccagtcaga ttatccttgg tgcagcggtg attgacgata ttctggggtt gattgtcctg
     1741 agtattgtca gcagtctggc acgtggtggg atcaactatg ctgaacttgg ggtgacggcg
     1801 gggctttcaa ttgggtttac catatttgtg atggtggtgg gaacgcgcct ggttaaacga
     1861 gcccaacccc aaatcgaaaa tctgaaagta agtgaagcct acttcattgt tggaatttta
     1921 ttgtgccttg ggttgtcgtg gttgggcacg ctggttggcg tcgcggcctt aattggcgct
     1981 ttcctggcag gaatggccct gggtgaatcc actgaacaca ccaacttgca cgctcaagcc
     2041 agctccgtga tggagttttc agtccctttt tttctggtca gcattgggct tcaacttgat
     2101 ctgagtgttt tcaaagacgc tcaggtgatt gcactggctt tagtgacaac gctggttgcc
     2161 gttgctacca aactggtcgc ttgtggtctg gtggcgttgc caatggggaa acgaacggct
     2221 ttacaagtgg gaatgggaat ggtgccacgt ggagaagtcg gtatcatcgt ggctcaaatt
     2281 ggcttgaaaa gtcaggtttt aaccccggcc ctgtacgccg ttgtggtttt tatggcggtt
     2341 gcgacaacgc tggttgctcc accgtttctg gctgctttat ttcaaccaag cccc
//
LOCUS       DAOQDO010000902         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39813_length_2394_cov_2.040616, whole genome shotgun sequence.
ACCESSION   DAOQDO010000902 DAOQDO010000000
VERSION     DAOQDO010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39813_length_2394_cov_2.040616"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKZ53_14120"
     CDS             <1..993
                     /locus_tag="PKZ53_14120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNJ41625.1"
                     /translation="DHSPLLSQSRTTEWKRKQVSLADFLPLADDMASMGSVEAVILSG
                     MGDPFVNQDIYSIIRVCKQYGWHVTTLTNALLADPARILELDVDMMLISVNGVSERSY
                     TAFHPNLTARDFARLGSLLERFATAGKRFKHVQVINCDTANEVVEMVRFAARYNAEHI
                     TYKLASLGHGTERCAITELQREQLLSDLIPTARSEAQKLDVLTNLDVFEAQVRTGGLA
                     TAPMADIGCFMGWTYARVTVDGTILFCCSTEVEVGHLSRGTFSEQWYGPQWNTVRQQL
                     MAGEYFSSCSQCGKLNQNVKIGKKVKEKFGELVYLHRTGQTHKAQGAGLKVEGL"
     gene            1051..1884
                     /locus_tag="PKZ53_14125"
     CDS             1051..1884
                     /locus_tag="PKZ53_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ41626.1"
                     /translation="MRKPTIEWQTNGLCNYDCTYCTQSRKFRQGHPTDDELDRFLSFF
                     ETLPGVWEIKMSGGEPFAFKGFMTRVIPGLAGLRHRVSVLTNLSAPPAILRKFVDLVG
                     DKLEIVSASLHLEYVTAEEFIEKAVLLRSWLRPETSLVVNNVLVPGTLDQLIEVRQQI
                     EEAGLRYFPQVMKTKHGVFPYSAPDQSRVVHITGKNPTSREANLAPSYRGRLCWTGVE
                     YFVLDQRGNAYSCRTAKRFGEGYLGNVLDGSFALKTEPARCTYSICPCTVPANRGMIQ
                     E"
     gene            2029..2286
                     /locus_tag="PKZ53_14130"
     CDS             2029..2286
                     /locus_tag="PKZ53_14130"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNJ41627.1"
                     /translation="MQLKTLAGIVTTRWRDPQTCEACDQPFVCGATLTGCWCTEIKLT
                     DAHRAALKTKFKHCLCRSCLEQFAQTDPNSILLQEKTECLP"
     gene            2274..>2394
                     /locus_tag="PKZ53_14135"
     CDS             2274..>2394
                     /locus_tag="PKZ53_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ41628.1"
                     /translation="MFAVIMAGGSGTRFWPASTDTMPKQFLPITSQQTMFEETV"
BASE COUNT          583 a          595 c          624 g          592 t
ORIGIN      
        1 gatcattcgc cgttgctgtc acaatcacga acgacagaat ggaaacggaa acaggtttcg
       61 ctggctgact ttcttccact ggctgatgac atggcttcga tggggtcggt ggaagcggtg
      121 attctctcag ggatgggcga cccgtttgtc aatcaggaca tttattcgat tattcgggtg
      181 tgcaaacagt acggctggca tgtgacaacg ctgaccaatg cgttgctggc ggatccggcg
      241 cgaatcctgg aactcgatgt ggatatgatg ttgatttcag tgaatggtgt gagtgagcgg
      301 tcctacacgg cttttcaccc aaacttgacg gcacgtgatt ttgccagatt gggtagcctc
      361 ctcgaacggt ttgcgactgc cgggaaacga ttcaaacacg tgcaggtcat caactgtgac
      421 acggcgaatg aagtggttga aatggttcga tttgccgccc gttacaacgc cgaacacatt
      481 acctataaac tggcgagcct gggtcacggc accgaacgct gtgccattac cgaacttcaa
      541 cgcgaacaac tgctttccga tctgattcca acagcccgaa gtgaagctca aaaactcgac
      601 gtgttgacca atctggatgt atttgaagcc caggtccgaa ctggcgggct cgccaccgca
      661 ccgatggccg atattggatg ttttatgggc tggacctatg cccgcgtaac tgttgacggg
      721 acgatcctgt tttgctgttc caccgaagtc gaagtcggtc atctgtcgcg cggaacgttt
      781 tctgaacaat ggtatggacc gcaatggaat actgtccgtc agcaactgat ggcaggcgag
      841 tatttttcaa gctgttcaca gtgcggaaaa ctaaatcaaa atgtcaaaat tggaaaaaaa
      901 gtgaaggaaa aatttggcga acttgtgtac cttcaccgaa cgggacaaac ccacaaagct
      961 cagggcgccg ggctcaaggt tgagggttta taaagacaaa aaggacgaaa aagacttccc
     1021 ttcgaaaacc ctgaccctga tttcttccct atgcgcaaac caaccatcga atggcaaacc
     1081 aatggcctgt gcaattacga ctgcacctat tgcactcagt cgcgcaaatt tcgccagggg
     1141 catccaaccg atgacgaact tgaccgcttt ctcagctttt ttgagacctt gcccggcgtg
     1201 tgggaaatca aaatgtcagg cggggagcca tttgctttca aaggatttat gacacgggtc
     1261 attccagggc ttgctggttt gcgccaccgc gtgtcggttc tgaccaatct ctcggcgcca
     1321 ccggcgatcc tgcgcaaatt cgttgatctg gtcggagaca aacttgagat tgtctctgcc
     1381 agcctgcatc tggaatatgt gacggctgaa gaatttattg aaaaagccgt gctcctgcga
     1441 agctggctgc gacctgaaac gtcactggtc gtcaacaacg tgctggtgcc gggaacactc
     1501 gatcaactga ttgaggttcg tcagcagatt gaagaggccg ggttgcggta ctttccacaa
     1561 gtgatgaaaa ccaaacacgg agtctttcca tattcagcgc ctgatcaatc gcgggtggtg
     1621 cacatcaccg ggaaaaatcc gacttcgcga gaagccaacc tggcgccatc ctatcgtggc
     1681 cgattgtgct ggaccggagt tgagtatttt gtgctcgatc aacgcggcaa cgcctattcc
     1741 tgccgaactg cgaaacggtt tggcgaaggc tatctgggaa atgtactcga tggcagtttt
     1801 gctctgaaaa ctgaacccgc acggtgtacc tattcgattt gcccctgtac cgttccagct
     1861 aaccggggga tgattcaaga atgagtcagg agttcgctaa gtttatttga ttgaatcatt
     1921 tgactgttga atttggtaaa ctcctgattt tcttccgtgt tttccgtgtt ttccgtggtt
     1981 aaactgtctg gaaattttcg gtaaggtact tatttggagg ccaacccaat gcaactcaaa
     2041 accctggctg gaatcgtcac gacccgctgg cgcgaccctc aaacgtgtga agcctgcgat
     2101 cagccatttg tctgtggcgc tacgctgacc ggctgctggt gtactgaaat caaattgacc
     2161 gatgctcacc gggcggcgct caagacaaaa tttaagcatt gcctgtgccg atcctgtctg
     2221 gaacaatttg cccaaactga tccgaactct attttgcttc aggagaaaac cgaatgtttg
     2281 ccgtgattat ggctggtggc tctggcacgc gtttttggcc ggcgagcacc gacaccatgc
     2341 ccaaacagtt tttacccatt accagtcagc aaactatgtt tgaagaaacc gtgg
//
LOCUS       DAOQDO010000903         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39821_length_2393_cov_3.636869, whole genome shotgun sequence.
ACCESSION   DAOQDO010000903 DAOQDO010000000
VERSION     DAOQDO010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39821_length_2393_cov_3.636869"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1389
                     /gene="nuoD"
                     /locus_tag="PKZ53_14140"
     CDS             121..1389
                     /gene="nuoD"
                     /locus_tag="PKZ53_14140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNJ41629.1"
                     /translation="MPDIATTTGNTVVDLDYAPQDKLAQHIREQSALDNTVTISMGPQ
                     HPSTHGVLRLQLELDGETVVQAIPDIGYLHTGMEKQMETKKYQQNIVITDRMDYLNPM
                     GNNLGYVMTVEKLLGIVDDIPPRAQTLRVLLTELQRIASHLVWLGTHALDLGAMSMFF
                     YCFREREKILNIYEAVCGGRMTVSYFRVGGLPYDVPPGFTKMVRQFLEDFPAAIKEYH
                     ALLTGNQIFQKRTRGVGHISAEDAINLGVTGPSLRGSGLPHDVRRSEPYCGYETYDFD
                     IPVEQDGDIFARYMVRMRELHESWKICRQALDRLKPGPVKANTPKVVLPEREEMKHQM
                     DALIHHFLLAAYGFSVPAGEAYHAIEGSKGELGFYIISDGTERPCRVHVRGPSFVNLQ
                     ALPKIVEGTMIADVVAVIGSLDIVLGEIDR"
     gene            1594..2058
                     /gene="nuoE"
                     /locus_tag="PKZ53_14145"
     CDS             1594..2058
                     /gene="nuoE"
                     /locus_tag="PKZ53_14145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNJ41630.1"
                     /translation="MFSEQINKKADEIIARYPVKRSAILPLLHLVQNEENQISKDGMR
                     FVAKKLGVEMNDVYEVLTFYTMYFTEPIGKYHIQVCRTISCYLCGAEDVTAHLVKRLG
                     IKPGQRTEDGRFRLSEVECLGSCCTAPMFQINFDYHENLTPEKIDKILDSLP"
     gene            2178..>2393
                     /locus_tag="PKZ53_14150"
     CDS             2178..>2393
                     /locus_tag="PKZ53_14150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681904.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNJ41631.1"
                     /translation="MEMKLLTKRFGMPNSQSIDTYLATEGYQALKKVVTTMTQDQVID
                     EVKKSALRGRGGAGFPTGLKWSFVPRQS"
BASE COUNT          641 a          597 c          606 g          549 t
ORIGIN      
        1 aaccaagaac caagaaccaa gaaccaagaa ccaagaacca agaaccaaaa accaaaaact
       61 aagcaccaca aacaccagtc gccagacttc cgttaaccgt caaccatcag gaaaaaatat
      121 atgcctgaca ttgccacaac caccgggaat acagtggttg atctcgatta tgcgccacaa
      181 gataagctgg ctcagcatat tcgcgaacaa tctgccctcg acaatactgt taccatcagc
      241 atgggccctc agcacccgtc aacccatggg gtgttgcgcc tgcaactgga acttgacggt
      301 gaaaccgtcg tccaggcgat tccagacatc ggctatctcc acaccgggat ggagaagcag
      361 atggaaacca agaagtacca gcagaatatt gtcattactg accgcatgga ctatctcaac
      421 ccgatgggga ataacctcgg gtatgtgatg acggtcgaaa aactgctcgg gattgtggat
      481 gatattccgc cgcgagccca gaccctgcgc gtgctgctga ctgaattaca gcgcattgcc
      541 tcacatctgg tctggctggg aacacacgcg ctcgacctgg gagcgatgtc gatgtttttc
      601 tattgcttcc gcgagcgtga aaaaattctc aacatttatg aagctgtttg cggtggtcgc
      661 atgacggtga gttacttccg cgtcggtggg cttccgtatg atgtcccgcc gggctttacc
      721 aaaatggtgc gccagttttt ggaagacttc cctgcagcga tcaaggaata tcacgccttg
      781 ttgaccggca accagatttt ccagaagcga acccgtggag ttgggcatat cagtgccgaa
      841 gatgccatca atctcggggt gactggcccg agcctgcgtg ggtcgggatt accgcacgac
      901 gttcgccgga gcgagcccta ttgcgggtat gaaacctatg attttgatat cccagttgag
      961 caggacggcg atatttttgc ccggtatatg gtgcgcatgc gcgaactgca cgaaagctgg
     1021 aaaatttgcc gtcaggcgct ggatcggtta aagcccggcc cagtcaaagc caacacaccg
     1081 aaagttgtgt tgcctgagcg ggaagaaatg aagcatcaga tggatgcgtt gattcaccac
     1141 ttcctgctgg cggcctatgg gtttagtgtg ccagccggcg aagcctatca cgccatcgaa
     1201 ggctcaaaag gcgagcttgg cttctatatc atcagtgacg gtacagagcg accctgccgg
     1261 gtgcatgttc gcggaccgtc atttgtgaac ctgcaggccc tgccgaaaat tgttgaagga
     1321 accatgattg ccgacgtggt ggcggtcatt ggcagcctcg acattgtgct cggagaaatc
     1381 gaccgctgag gaagaatgag gaatgaagaa ttgagaatga agaattgaga atgaagaacg
     1441 taacaaaagc gttctgagta gattgccaac cagctatcga actgctctcg atcaactcat
     1501 aattctcaat tcttcattct tcattctcaa ttctttattt tcaactcagg attcatcatc
     1561 cttctcattc ctttgttccc cgcttagatc actatgttta gcgaacaaat caacaaaaaa
     1621 gccgacgaaa tcattgcgcg gtaccctgtg aaacgctctg ccattctgcc tctgctgcat
     1681 ctggtccaga atgaagaaaa ccagattagt aaggatggca tgcgatttgt tgccaaaaag
     1741 ctcggagttg aaatgaacga cgtgtacgaa gtgttaacct tctacactat gtattttact
     1801 gaaccgattg gcaaatatca tattcaggta tgccggacaa ttagttgcta tctgtgcggt
     1861 gccgaagatg ttaccgccca tctggtcaag cgattgggga tcaaacctgg gcagcgcact
     1921 gaagacgggc ggttccggtt gagcgaagtc gagtgtttgg gatcgtgctg tacggcaccg
     1981 atgttccaaa tcaactttga ctaccatgaa aatctgacgc cggaaaaaat cgacaagatt
     2041 ttggactcgc tgccataaga aattgaattt attcagactt ttcgtcgtgc tctgggccca
     2101 gtacctgccg gatgccagac gctgtgtatg gcaaagaaag ctggtattca gggcaaacga
     2161 taccatttga ggcaatcatg gaaatgaagc tgcttaccaa acgatttggg atgccaaact
     2221 cccaaagcat tgatacctat ctggcgactg agggctatca ggcactcaaa aaagtcgtga
     2281 ccaccatgac ccaggatcag gtcattgatg aagtcaaaaa atcagccctg cgcggacgcg
     2341 gtggcgctgg cttcccaacc gggctcaaat ggagttttgt cccgcgacag tcc
//
LOCUS       DAOQDO010000904         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39830_length_2393_cov_2.890077, whole genome shotgun sequence.
ACCESSION   DAOQDO010000904 DAOQDO010000000
VERSION     DAOQDO010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39830_length_2393_cov_2.890077"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKZ53_14155"
     CDS             complement(<1..1093)
                     /locus_tag="PKZ53_14155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41632.1"
                     /translation="MAVTQEKLNGLTRVRTIGEGTFGTTWLAKHEEFNTEVVIKEIHS
                     LKQKLEPVVQESIRWSKLEHHPNIVPITEIDVQGDTLAMVMPFEPGGSLEDWLRRYGG
                     KAESVGEAVDLLTGILNGLEHLHKQGITHGNLKPSNIMIRRGTPRLTEFGLVPVIQPD
                     LQNTARIETLWYSAPESLGGFFSPQTDLWSAGVIFYRLLAGRLPFPHDNRDELLWAIL
                     TQDPDPLPRIVARSFYKFLKRALHKESFPRYWKASEMQSDLLMAFEYSIMPDAELGSD
                     TPLPVIKPTDIASLPTDALGRAPETIVMESLPAETLPPQETIQLEKPELPISEASPAV
                     PEEADASVTFPSEPTGSIKLRTTQEILPQY"
     gene            1491..2006
                     /locus_tag="PKZ53_14160"
     CDS             1491..2006
                     /locus_tag="PKZ53_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ41633.1"
                     /translation="MDTTSPFLTLEELSATVGQLLDRYGLRDAQRDARVSAIPDARTI
                     RYYTTLGLLDSPTVQGRQARYQRRHVLQLLAIKALQGASLTLGEIQERLYGRTDEELE
                     QLAASLAQQQSDAVELRPVVWREIVIEPGLKIVADEHWFPGNDLEATLNRIRAALTVL
                     QASDQRGKRRE"
     gene            2009..>2393
                     /locus_tag="PKZ53_14165"
     CDS             2009..>2393
                     /locus_tag="PKZ53_14165"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNJ41634.1"
                     /translation="MTAPVVIPMIEVIEEIRRCQVGVLTVTQAARPVTLPLAKVDISA
                     RVADRVAHVTMLETFRNPHQEHLEAVYTFPLPGSSVVSSFEMKVGDRTIHGVVKERGE
                     ARRSYQQALEAGRRAALLEQERDDIF"
BASE COUNT          569 a          648 c          612 g          564 t
ORIGIN      
        1 agtactgggg cagaatttcc tgagttgttc gcagcttgat tgagccggtt ggctcagaag
       61 gaaacgtcac tgaagcgtca gcttcttcag gcactgccgg agacgcttcg gaaatcggga
      121 gttccggttt ttcaagctgg atggtttcct gtggtggcag cgtctcggca ggtaatgact
      181 ccatgacaat ggtttcaggt gcccggccca gcgcatcggt cggaagtgac gcaatatcag
      241 ttggtttgat cacaggcaac ggcgtatctg acccgagttc ggcatcgggc ataatgctgt
      301 attcaaacgc catcagcaaa tccgactgca tttcacttgc tttccagtac cgtggaaaac
      361 tttctttatg gagcgcccgc ttcaagaact tataaaagga tcgggcaaca attcggggca
      421 acgggtctgg atcctgcgtc agaatcgccc agagcaattc gtcgcgattg tcgtgtggaa
      481 acggcaggcg tcccgccagc aaccgataga aaatgactcc agccgaccat aaatccgtct
      541 gtggtgagaa aaagccgccc aatgattccg gcgccgaata ccacagcgtt tcaatccggg
      601 cggtgttttg caaatccggt tgaatgacgg ggaccagccc aaactcggtt aatcgcggtg
      661 ttccccgacg aatcatgatg ttggagggct tgaggttccc gtgagtaatg ccctgtttgt
      721 gtagatgctc aagtccattc aaaatcccgg tcagaagatc aactgcctca cccaccgatt
      781 cggcttttcc accatagcgc cgaagccagt cttcaagcga tccgccaggt tcaaacggca
      841 tcaccatggc cagggtatcg ccctggacat caatctctgt gattggaacg atatttgggt
      901 gatgttccag tttggaccac cgaatacttt cctgcaccac gggttccagc ttctgcttta
      961 agctgtgaat ttctttaatc acaacttcgg tattgaactc ttcgtgtttg gctaaccacg
     1021 tggtcccaaa ggtcccctcc ccaattgttc gtaccctggt caatccattt aacttttcct
     1081 gcgtcacagc catgaccatc ctcagctact tagaatcaac ggtatcgtgc tcaattccga
     1141 aatatcacct cccccagatg aatcagccca gagcctggaa cgggaacgcc accattgccc
     1201 gcctggacag ccactggtgt cagttccact caattttcct gaatcagatt caaggtggtg
     1261 gtgatgtttt ccctggtaag cagcgatagc ctttgtttgt gaaggctaca atactcttca
     1321 aaatgagcag attatgcaag gtttcctgag aaaaaacgtg agattgttgg aaatagtgag
     1381 gaagcaaaag agcgaagagt gaacgacctg gcgacgtcgc tatttcccaa ttcactcttt
     1441 cattcattta tcttgacagt attgcagtaa ttctgtaaat tactgtcgtt atggatacga
     1501 catcaccatt ccttaccctt gaagaacttt cagccaccgt cggtcagttg cttgaccggt
     1561 atggcttacg cgatgcccag cgggatgcca gggtgtcggc aataccagat gcccggacca
     1621 tccggtatta caccacgctc ggcctgcttg attctccgac ggtccagggg agacaggccc
     1681 gctatcaacg ccgacatgtg ctgcaactcc ttgccatcaa agcgttgcag ggcgcatcgc
     1741 tcaccctggg ggaaatccag gaacgactct atggacgcac cgacgaggag cttgaacaac
     1801 tcgcagccag tctggcccaa cagcaatcgg acgccgttga acttcgcccc gtggtgtgga
     1861 gagaaattgt cattgaacct ggattgaaaa tcgtggctga cgaacattgg ttcccaggca
     1921 atgatctcga agccaccctc aaccgaattc gcgcggcctt gaccgtgctt caagcctcag
     1981 accaacgagg caaacggaga gaatgatcat gactgctcct gtggtaattc cgatgattga
     2041 agttatcgaa gaaattcgcc gctgtcaggt cggtgtcctg accgtaaccc aggcggcccg
     2101 accagtgacc ctgccgctgg ccaaagtgga tatttcggcc cgtgtcgcag atcgtgtggc
     2161 gcatgtcacg atgctggaaa catttcgcaa ccctcaccag gagcacctgg aagcagtgta
     2221 tacatttcca cttccgggca gttcagttgt ttcaagcttt gaaatgaaag ttggcgaccg
     2281 gaccatccac ggcgtcgtca aggaacgtgg cgaagcccga cgcagctacc agcaggcact
     2341 tgaggcaggc cgccgggccg ctctgctcga acaggaacgt gacgatattt tca
//
LOCUS       DAOQDO010000905         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39893_length_2391_cov_2.918236, whole genome shotgun sequence.
ACCESSION   DAOQDO010000905 DAOQDO010000000
VERSION     DAOQDO010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39893_length_2391_cov_2.918236"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKZ53_14170"
     CDS             <1..746
                     /locus_tag="PKZ53_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ41635.1"
                     /translation="ISESMARTCWGEADPIGKQLRYVRGAQPGPWFTVVGVVSDIKFQ
                     GRLTDDVDQRDIYVSIQQVPSLDPRFVLLALKYQGETADAVTAVQSAIQSVDPLLPVD
                     QISTMTERLRDELNESRFVAQLSTLFAGIALMLSLLGVYGVLSWWVSERTSEIGIRVA
                     LGAQSHDILNLVLKRAMLLTGVGIVIGLAGARVWDRWLQSLVNSQNAIDGNLLGLMVI
                     FLVMTSLVTSIIPAWRATQVDPLITLKRE"
     gene            complement(866..>2391)
                     /locus_tag="PKZ53_14175"
     CDS             complement(866..>2391)
                     /locus_tag="PKZ53_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41636.1"
                     /translation="TELDVLNQELEQKVETRTHELAVANGQLETAVEQLRDSETAALK
                     AKAEAERANRAKDIFLANMSHELRTPLNAVLGFAQVMNRTASRPAQDREYLSYITHSG
                     EHLLGLINDVLSIAKIESGRLTLTEQSFDLRELVSSLQDVFRLQVADKGLKLQVEISP
                     SVPALLGGDAGKLRQVLMNLIANAVKFTREGSVTVRIDAQEAGFRFEVADTGIGIAPE
                     DVKKLFQPFSQVGELAGQSQGTGLGLAISQKLVHLMGGELDVTSQPGTGSVFSFWLPL
                     TAAQFETRSLQHQILALAPNQPRWQILVVDDRLENRRVMETLLQTVGFDVQTVATGEQ
                     ALERYCQNRPDMVFLDLRMPGMDVFEVVRRLRATETDLHSTRLPIIAVTASAFEEDRH
                     MVLASGFDDYLAKPFTEKMLFEMIGKHLKVQFQEEITHVTPSSFDQPSDLTQQNGLDE
                     EWKAAFHRSLTTGRTAKALELIEQLPPHHQSLADTLRRLVKQYRFDELFALLEHEPG"
BASE COUNT          533 a          622 c          637 g          599 t
ORIGIN      
        1 tcatcagcga atcaatggcc cgcacctgtt ggggcgaagc tgatccaatt ggaaaacaac
       61 tccggtatgt tcgcggggcg cagcctgggc cctggtttac tgtggtggga gtcgtatcag
      121 atatcaaatt tcagggacgc ctcaccgacg acgttgatca acgcgatatc tatgtttcaa
      181 ttcagcaagt tccgtccctt gatccacgat ttgttttact ggcgctgaaa tatcagggag
      241 aaactgccga tgccgtgaca gccgttcaat cagccattca gtcagtagat ccgctgcttc
      301 cggtggacca gatcagcacc atgaccgaac gtttgcggga tgaactcaac gaatcacgat
      361 ttgtggcgca gctttcgacc ctgtttgctg gaattgccct gatgttgtcg ctgctggggg
      421 tctatggcgt gttgtcgtgg tgggtttcgg agcgaacttc ggaaattgga atccgggtgg
      481 cactcggtgc ccagtctcac gatattttaa atctggttct caaacgggcc atgttactga
      541 caggagtggg cattgtcatt ggattagctg gcgctcgggt atgggatcgc tggctgcaaa
      601 gcctggtgaa cagccagaat gcgatagatg ggaatttact tggattgatg gttatctttc
      661 tggtgatgac ttcgctggtg acaagcatca tcccggcctg gcgcgctacc caggttgatc
      721 cattgatcac tctgaagcga gaataatcta ttttggggtc agggggcaga ccgggaaaag
      781 gacataaagg acataaagga caaatcttcg aaatccctga cccctgaccc ctgacccctg
      841 acccctgacc cctgacccct gacccctacc ctggctcgtg ttccagcaag gcaaaaagtt
      901 catcaaaccg atactgtttg accagtcggc gaagcgtgtc tgccagcgac tgatgatgcg
      961 gggggagttg ctcgatcaac tccagggctt tggcggttcg gccagtggtt aaactgcggt
     1021 gaaatgcagc tttccattct tcgtccaatc cattttgctg agtgaggtcc gatggttgat
     1081 caaagcttga tggagtgaca tgtgtaattt cttcctggaa ttgaactttc aggtgtttgc
     1141 caatcatttc aaacaacatc ttttcagtaa acggcttggc cagatagtcg tcaaatccag
     1201 aggccagaac catgtgccgg tcttcttcaa aggcactggc ggttacggca atgattggaa
     1261 gtcgcgttga gtggaggtcg gtttcagtgg ctcgtaagcg ccgtaccact tcaaacacat
     1321 ccattccagg cattcgcagg tccagaaaca ccatatccgg gcgattctga cagtagcgtt
     1381 caagcgcctg ttcaccggtt gcaaccgtct ggacgtcaaa cccaactgtt tgcaacaacg
     1441 tctccatcac gcgtcggttt tccagccggt catctacgac caggatttgc cagcgtggtt
     1501 gattgggcgc cagtgccagg atttgatgct gaagcgatct ggtttcgaat tgtgccgccg
     1561 tcagcggaag ccagaatgag aatacactcc cggtccctgg ctggctggtg acatctaatt
     1621 cgccacccat cagatgaacc agcttctgac tgatggccag cccaagtccg gtgccctgcg
     1681 actgaccagc cagttctcct acctgtgaaa atggttggaa gagttttttc acatcctcgg
     1741 gagcaatccc aatccccgta tctgccacct caaatcgaaa tccagcctcc tgggcatcaa
     1801 tgcgcactgt cactgaaccc tcgcgggtaa atttgacagc gttggcgatc aggttcatca
     1861 gaacctgacg cagttttcca gcatctccgc ccagtaaagc cgggacactt ggcgagattt
     1921 caacctgtaa tttcaatcct ttatcggcga cttgaaggcg aaaaacatcc tgcaaactgc
     1981 tgacgagttc gcggagatca aaggattgtt cggtcaacgt caaccgaccg ctttcaattt
     2041 tggcaatcga gagcacgtca ttgattaacc ccagcaaatg ctcaccgctg tgggtgatgt
     2101 agctgagata ttcacgatcc tgagctgggc gactggctgt ccgattcatc acctgggcaa
     2161 aaccgagcac cgcattgagc ggagtgcgga gttcgtggct catattggcc aggaaaatat
     2221 cttttgcccg gttggcgcgt tccgcctcag ctttggcctt gagggcagcg gtctcagagt
     2281 cgcgtaattg ttcaaccgcg gtttcaagct gaccattggc gacggccagt tcatgggttc
     2341 gggtctcgac tttctgttcc agttcctggt tcaagacatc gagttcggtg t
//
LOCUS       DAOQDO010000906         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_39912_length_2390_cov_3.659529, whole genome shotgun sequence.
ACCESSION   DAOQDO010000906 DAOQDO010000000
VERSION     DAOQDO010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39912_length_2390_cov_3.659529"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKZ53_14180"
     CDS             complement(<1..641)
                     /locus_tag="PKZ53_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNJ41637.1"
                     /translation="MSDLLNNPHSATNEQSQILVGEMGLTETLIGRFEMAAEKLNLDS
                     NLRTLLRVPGREFVVNLPVHMDNGEIRVFESYRIQHHIASGPMQGGLRYRPDITLDEM
                     RALSLWSTYTCAVVNVPFGGSMGGILCDPKELSQSELERLTRRYISEMLDILGTERDI
                     LAPDLSTDQQVMAWIMDTYSMHARHTVRSIVTGKPFEMGGSHFAREAIGYGAT"
     gene            1378..2316
                     /locus_tag="PKZ53_14185"
     CDS             1378..2316
                     /locus_tag="PKZ53_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ41638.1"
                     /translation="MKCPECGTEAREGTRFCRSCGELLTDDTGPLTNPPLATQPAPAL
                     APAPVFPPAPPPINPVPISPPAPLPGSGTQPFTSGMPPVAVPTGKMAPMAPLPSPPPS
                     SPGEHRARATSGGGSLAPPPPAPIVRHKTTSKPLVIGAALAVMVLLGIAIGVGYRWGK
                     ETADKQLAATQNNWKSTSVPQKERGEMRINKSYLTRVEVSFMDVAPQNDTEIVFVESN
                     KGLQFPSGVKALVLQLQGNPPADTQLSIVWKQGSTVIQRTTNRDELNNLGRLPAGANF
                     GIYKTNRAAIEDGEYEVQIEEKGGELAAYAKFVIGK"
BASE COUNT          612 a          581 c          653 g          544 t
ORIGIN      
        1 caggtggcgc catacccgat ggcttcacgt gcaaagtggg aaccgcccat ttcaaaaggt
       61 tttccggtca caattgatcg cacggtatgg cgtgcgtgca tcgagtaggt atccataatc
      121 caggccatga cctgctggtc ggtgctcagg tctggcgcca ggatatcgcg ctctgtgcca
      181 aggatatcga gcatttcaga aatgtagcgg cgagtcagcc gttcgagttc tgattgggat
      241 aattctttcg ggtcacagag aatgcctccc attgatccgc caaacgggac attgacgacg
      301 gcacaggtgt aagttgacca cagtgacagg gcacgcattt catcaagcgt gatgtcgggg
      361 cgatatcgca acccaccctg cattggaccg ctggcaatgt ggtgttggat gcgatagctc
      421 tcaaagacgc gaatttcacc attatccata tgaaccggca ggttcacgac aaactcacgg
      481 cctggaactc gcaacaacgt tcgcaggttt gagtccaggt tcagtttttc ggcagccatt
      541 tcaaaacgcc caatgagcgt ttcggtcagg cccatttcac cgacaagaat ttgagattgt
      601 tcgttggtgg ctgaatgggg attgttcagg aggtcagaca tagggcgttc tatcctcgaa
      661 acttcaggct atgaaagaaa gatgaaaggc attggcaggc aaaacgaagc ctgccagtga
      721 agaatggatg cgaacccacg ggacgtaatg tgggggctcg aaagaacgaa aggaccagta
      781 ccggtgaatg aattgcagta tccagatgag cgattgatgg ctcggaggtg ccagtgcttt
      841 tgccccacat ctcagcctgg gtctctcaaa gaacaacaga ggtgcaatgg aaagatacgg
      901 tgcgagaaca atggcgtgag tgtcataaac caggtgtttg aggtgagccc ccgtacaact
      961 ggttcaattg gtgtgagact ttaatcgggc aagactttgt atcacatcag ttaatgtcag
     1021 gcaacacgaa gaaaaggggg atgaatgaag aattgagact gaagactgaa gaatgatacg
     1081 ttcttgattg tcggctggtg attcccaggc tcgacgtgga caactctcag attttcttct
     1141 ataaccgaga tgaaaacagg gaggatctct caggaaaatt gataaccccc aacctcacct
     1201 gatcacccaa tccgcgattt tttcttgcct gtcccattca atgaagtgct accattttga
     1261 aatgatcctt gctgtatgag caaaaggcta agctgttgat aaagcggtgt ttctatcagt
     1321 tcttagcctc atattctcag ccctgaatgg tatataacat ccgtctggag tgtgtttatg
     1381 aaatgccctg aatgtggaac tgaagcccgt gaaggaaccc gattttgtcg ttcgtgtggt
     1441 gaattattaa ctgatgacac ggggccatta acgaatccac cattggcgac tcaacccgcc
     1501 ccagctctgg ccccggctcc tgtttttcct ccagcgccgc cgcccatcaa cccggtacca
     1561 atttccccac cggcaccgct gccaggaagt ggcacgcagc cgtttacttc tgggatgcct
     1621 cctgtcgctg taccgactgg gaagatggca ccaatggcgc cactgccgag tccaccgccg
     1681 tcttcacctg gtgagcatcg cgcccgtgcg acatcaggtg gcggctcact cgccccaccg
     1741 cctccggctc cgattgtgcg tcacaagacg acatccaaac cactggtgat tggtgccgcg
     1801 ttggcggtca tggtgttgtt gggcatcgcc atcggggtcg gatatcggtg gggaaaggaa
     1861 acggctgata aacagcttgc tgccactcaa aataactgga aaagcacctc ggttccgcaa
     1921 aaagagcgag gtgagatgcg aatcaataag tcgtacctga cccgcgttga ggtgagcttt
     1981 atggacgttg caccccaaaa tgacactgaa attgtctttg tggaaagcaa taagggactc
     2041 cagtttccgt ctggcgtcaa agccctggtg ctccagcttc aagggaatcc gcctgctgat
     2101 acacaactca gtattgtctg gaaacaggga tcaactgtaa ttcagcgcac caccaatcgc
     2161 gacgaactca ataatctggg gcggttacca gccggtgcga attttgggat ttacaaaacg
     2221 aatcgggccg cgattgaaga tggggagtac gaagtccaaa ttgaggaaaa aggtggggaa
     2281 ctagctgcct acgcaaagtt tgtgatcggc aagtagggaa gaagaataca gaatgaagaa
     2341 ttgagaatga agaagaaccg ggttccgggt tccgggttcc gggttccggg
//
LOCUS       DAOQDO010000907         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40003_length_2387_cov_3.628216, whole genome shotgun sequence.
ACCESSION   DAOQDO010000907 DAOQDO010000000
VERSION     DAOQDO010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40003_length_2387_cov_3.628216"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="PKZ53_14190"
     CDS             complement(<1..2323)
                     /locus_tag="PKZ53_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41639.1"
                     /translation="MSSTFPLFWITIVFLLLVWLVAMVCGKRAAMTGSAESPLQRQVG
                     QILRALALITVIGSLASALNLFGLTVLVSGLLACVLLEHSWHIRRILSDFASVFEIWE
                     RVRNPDVWHRIRVRFWAWLKNQGSTWHQSILSPTILVWSGVAGLMAATFFFAKGQFAI
                     SNLRLLQAESYALLLQSKYTLFNTVTPDRIGNGLAVLTATVSLLSATDALHVVRMLGP
                     IIACWLAGATGYLVFWATGQKLAGIVTGCLVLTGILSGPIPGPWLQFAWPPEPAAAFG
                     LKLLTTCNEAVGRSLAGLPFEAALAVALCTWPALAASETPDQLWDGLAGISLTTLLHP
                     GMAVGLLLAALFFHVGVILVSQLALPVKRLFFGALAWGLAVGIGHGLILLQSHAQMRS
                     KALFSWLVPSPQTVPRVLPPVHWTGAGIIEALRTAPFFVFLAGVALVTALLLVVMVFS
                     RSGSGRTRLLSAGWLVFVWTGLVCWLGIDPLQPVPPVFQEILKGWPWIVICWIGLGVG
                     ALMNWIEAAFPRLTQVSLFTAFATLAVCGALVGASLWRGNLPPFQAQDQTLRFWCGDF
                     AQAGGLSLLEYDQSARIIQQITRHYPRGEWMVIAPTEQFIEVFGRGWHIDLFAWSQQF
                     TQDQVQSPEFRFPIELPDTFIFIEKRPWISDPRPQPSVVGFAQQTDITFRMYRSPAGR
                     SSLMQSTLNLLNAYGTHHTNLGVFFEDDHLVVFHLINQNSTRSLPFRLIRNWHETGGP
                     ERSPTMTKPMGCFSLGLIFLMWSTLSCAATSPPV"
BASE COUNT          651 a          648 c          645 g          443 t
ORIGIN      
        1 gtacgggcgg cgacgttgca gcgcatgaga gtgttgacca catcaggaaa atcagtccaa
       61 ggctgaaaca gcccattggt ttggtcatag tcgggctcct ttccggtcca ccggtttcgt
      121 gccagttcct tatgagtcgg aaaggcagtg agcgggttga gttttgattg atcagatgaa
      181 agacaaccag atggtcgtct tcaaaaaaca cacccaggtt ggtgtggtgg gtgccgtagg
      241 cgttgagcag attcaaggtt gactgcatca ggctgctgcg tccagcggga ctccggtaca
      301 tgcggaacgt gatgtcagtt tgctgggcaa atcctaccac agaaggttgt gggcgcgggt
      361 cggaaatcca ggggcgtttt tcgatgaaaa taaaggtatc aggtaattca atcggaaacc
      421 gaaactctgg tgattggact tgatcttgag tgaattgctg gctccaggca aagagatcaa
      481 tatgccagcc acgaccaaaa acttcgataa attgttcagt cggagcaatg accatccatt
      541 caccacgtgg atagtgcctt gtaatctgtt gaatgatgcg tgctgactgg tcatattcta
      601 agagtgagag gccaccagcc tgagcaaaat ctccgcacca aaaccggagt gtttgatcct
      661 gggcttgaaa cggtggcagg tttccacgcc agagagaggc gccgaccagg gccccgcata
      721 ctgccagcgt tgcgaaagct gtaaacagcg atacctgggt taatctggga aaagccgcct
      781 caatccagtt catcagggcg ccaaccccaa gtccaatcca gcaaatgaca atccatggcc
      841 atcccttgag gatttcttga aaaactggtg gcactggttg gagcgggtca atccctaacc
      901 aacacaccag acccgtccag acgaaaacca gccagcccgc actcaacagt cgagtacggc
      961 ctgaacctga ccgactgaag accatcacca cgagcagcag agccgtgacc agagcaactc
     1021 cggccaaaaa cacaaagaat ggtgccgtgc gcagggcttc gatgattcca gcaccagtcc
     1081 agtgaactgg gggaagtacc ctgggcacgg tttgaggaga ggggacgagc caggaaaaca
     1141 acgcctttga ccgcatttga gcgtgggatt ggagcaggat caacccatgc ccgataccta
     1201 cagccagccc ccaggccagc gcaccaaaaa aaagccgttt caccggcagt gccagttgtg
     1261 agaccaaaat gaccccaacg tgaaaaaata gcgctgccag taagagtccc accgccatac
     1321 ctggatgcaa cagcgtcgtc aggctgatgc cagccaatcc gtcccagagt tgatctggtg
     1381 tctctgaggc cgccagggct ggccaggtac acagggcaac cgccagcgct gcttcaaacg
     1441 gcaatccagc cagtgaccgc ccaaccgctt cgttacaggt ggtcaacaac ttgagcccaa
     1501 aggcggcagc cggttctggc ggccaggcga actgaagcca cggacctggg attggcccag
     1561 acagaatccc agtcaggacc aggcatccag ttacaattcc ggccagtttt tgacccgtag
     1621 cccagaaaac cagatagcct gtggctcccg ccagccagca cgcaatgatc ggcccgagca
     1681 tccgcacgac gtggagtgca tcagtcgcgc tcagcaggct caccgtcgcg gttaaaacgg
     1741 ccagcccgtt cccgatgcgg tcaggggtca ctgtattaaa cagcgtgtat ttgctctgaa
     1801 ggaggagggc gtaactttca gcctgcagga gtcgcaggtt ggagatggca aactggcctt
     1861 tcgcgaaaaa aaaagtagcc gccatcagac ctgcgacacc actccacacc agaatggtgg
     1921 gcgagagtat tgactggtgc caggttgatc cctgattttt caaccaggcc cagaaccgga
     1981 cccgaatgcg atgccagaca tccggatttc gaacccgctc ccaaatctca aacaccgatg
     2041 caaaatcact cagtattcgc cgaatgtgcc aggaatgctc aagcagtaca caggccagca
     2101 gaccagagac gagtaccgtt aacccaaata aattcagcgc tgaagcgaga cttccaatta
     2161 ccgtgatcaa tgccagggca cgaagtatct ggcccacctg tcgttgaaga ggcgattctg
     2221 ctgatccagt catcgccgca cgtttcccac aaaccattgc caccagccac accagcagca
     2281 ggaagacaat cgtaatccaa aaaaggggaa atgtactgga cacaacgagt tggaatgaag
     2341 aatgaagaat gaagaattga gaatgaagaa ttgagaatga agaattg
//
LOCUS       DAOQDO010000908         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40215_length_2380_cov_2.966452, whole genome shotgun sequence.
ACCESSION   DAOQDO010000908 DAOQDO010000000
VERSION     DAOQDO010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40215_length_2380_cov_2.966452"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            517..897
                     /locus_tag="PKZ53_14195"
     CDS             517..897
                     /locus_tag="PKZ53_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ41640.1"
                     /translation="MNFFVKSYESDGAQILILGGKLTIGAPVRELRQAIDARLATQTK
                     CIILNFSQLMFMDSSGIGELVGCNNKVKAAGGTLLLCELPNKVRDLFRITHVAKLFNI
                     HETESAAIESFLVGETKSATSDIR"
     gene            961..2115
                     /locus_tag="PKZ53_14200"
     CDS             961..2115
                     /locus_tag="PKZ53_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41641.1"
                     /translation="MNTRFWKRTCAATGLALALGFNTLSFAGFPSPLAANGTAVAPTR
                     AESVLSFLPQSDTVVLVDMNRLYNSVIFQMARDNPKFAKEMSEMESMAGTFGVDISKL
                     EYLAVGLTPKSGKPGDFDPTFVLSGAFNQEQLVTGLKNKSEGKVGTETYGSDTLYVLN
                     GGAGTDTAATKEKKVLTNDDFNGGSEPKPEKAPAASGSMKMSDMAFSFVNANTVVFGP
                     KANVIKSLDTRSGKSPSIASSKELVDGLDSADKVAAIRFATVVPASMRKELEKEIAND
                     PKAEMFIKPMLAVTHSFGAIDFTSGLKLDTTLRTPSADEGKPIFDQLNGLLMLGKMGM
                     GSSDKPNEKMMLDVLNQISILNKGRDVQIQLSVPEATLKTLLVGNQKAAQ"
BASE COUNT          625 a          559 c          577 g          619 t
ORIGIN      
        1 ggctgaatca attttcaact gaaaccctgg tcaaaatcag tcatcccagt ctcgaacaag
       61 ggacgggtcg aattctaaat cgaaccacag gagattggaa cacatcgggt tcagggttca
      121 gggttcaggg tttgatccga acccctcgaa ctggccggca attcagcgag gctcaaccca
      181 atggtctgaa cctgaaatca atcaagagtg ttccaatccc gtgtggactg attgagtcgg
      241 aagggctgag agaaaaccag ggctcagggc tgaagaagtg gttttctttc atccctcatc
      301 cttcatccct catcccttcg attgccctga gcccagtttc ttcagcgcga aggtctctcc
      361 ttgacggatg ggccgggctg gctcagactg aagagtgttt ttattcgttg caataaacga
      421 ccggctctga tgaagaccga ttgggttgaa tgccgactca attcaatgct ttcagtctta
      481 atcggtttga gattgaaata tgcgaggtat tgaactttga atttctttgt aaagtcctat
      541 gagtcagatg gtgcccagat tctgatatta ggtggcaaat taaccattgg ggcaccggtg
      601 cgtgagttgc gccaggcaat tgatgcccgg ctggcaaccc agaccaaatg tattattctc
      661 aatttttcac aactgatgtt catggatagc tcggggattg gtgaactggt tggatgtaat
      721 aacaaagtga aagcggctgg gggaaccctg ttgctctgtg aattgccgaa caaagtccgc
      781 gacctgtttc gtatcacgca cgtcgcgaag cttttcaaca tccacgagac cgaatcagcc
      841 gctatcgaat ccttcctggt cggggaaacg aagagcgcca catccgacat ccgatagagt
      901 gcaattaaac ccactcctca tacttcaacc actctcgttt gaaaaaggat tcacaacttt
      961 atgaatacac gtttttggaa gaggacttgt gccgctactg gtctggcgct ggcacttgga
     1021 ttcaacaccc tgtcttttgc tggtttcccc tccccattgg ctgcgaatgg gactgccgtc
     1081 gcaccaaccc gcgccgaatc tgttctttca tttctgcctc aatcagatac ggttgtgctc
     1141 gttgatatga accggctgta caactcagtg atttttcaaa tggccaggga caatccaaaa
     1201 ttcgcaaaag agatgtctga aatggaatcc atggccggaa cctttggagt tgacatttcc
     1261 aagcttgaat atctggcggt tggcctaacc ccaaaatctg gtaagccggg tgatttcgac
     1321 cccacctttg tgctcagcgg ggcctttaac caggagcaac tggtcaccgg gctcaaaaac
     1381 aagtctgaag ggaaggttgg aactgaaacc tatggttcag acacgcttta tgtgttgaac
     1441 ggcggcgctg gaaccgatac ggctgcgacc aaagagaaaa aagtcctgac caatgatgat
     1501 ttcaatggtg gcagtgagcc aaaacctgaa aaagccccgg ctgcctcggg ttcgatgaaa
     1561 atgtctgaca tggcctttag ctttgtgaat gccaacaccg tggtttttgg acccaaagcc
     1621 aatgtcatca agtcgcttga tacccgttct gggaaatcac cgtcaattgc gtcaagcaaa
     1681 gaactggttg atggcctgga ttcagcggat aaagtggcgg ccattcgttt tgccacggtt
     1741 gtcccagcca gtatgcgcaa agaacttgaa aaagaaattg ccaatgaccc caaagctgaa
     1801 atgtttatca aaccaatgct ggccgtcacc catagctttg gcgcgattga ttttacctct
     1861 ggattaaaac tggacaccac cttgcggacg ccgtcagccg atgaaggcaa accaattttt
     1921 gatcaactca atggattgtt gatgttgggc aaaatgggga tgggcagcag tgataaacca
     1981 aatgagaaaa tgatgcttga tgtcctgaat caaatttcga ttttgaataa aggacgtgat
     2041 gttcagatcc aactgagtgt tcccgaagcc acgttgaaaa ctttactggt cggcaaccag
     2101 aaagccgcac agtaagcagc cgttttaaac caggcataag atggaaaaag gagggtgggt
     2161 taaaaaacct gccctctttt tttatttatg tagctgaggc atggttcttt cttcaaaaaa
     2221 atgtgtaatg ttttggcatt cgccgtcact accggtttag tccacgagtt tttccctttc
     2281 aagaatcgtt caatatctga ttccaggtaa gtcccctggc ctggacagag tttggccaac
     2341 acgtatccac aattctgttt cctgattttt caaccggttt
//
LOCUS       DAOQDO010000909         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40359_length_2376_cov_3.405429, whole genome shotgun sequence.
ACCESSION   DAOQDO010000909 DAOQDO010000000
VERSION     DAOQDO010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40359_length_2376_cov_3.405429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKZ53_14205"
     CDS             complement(<1..403)
                     /locus_tag="PKZ53_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNJ41642.1"
                     /translation="MSGHSKWHTIKHKKGALDAKRGKITTRMVKEITVAARMGGGDPG
                     SNARLRKAVSDAKAENVPNDTIDKAIKRGTGEIEGLTYEEITYEGYGPAGVAIMVQAT
                     TDNRNRTVSELRHLFSKNGGNLGESGCVGWMF"
     gene            905..1291
                     /locus_tag="PKZ53_14210"
     CDS             905..1291
                     /locus_tag="PKZ53_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41643.1"
                     /translation="MSHRILIVDDEMDTAQMLRVILESRNYLCSIASNGKDGLQLILD
                     EKPDLVLMDYMMPIMTGVEAFKLLRRIESSTSNRPTPVIMLTAKSDNEDEQKELLDLG
                     LRAYITKPFRYQMLLETIGKIVAEHH"
     gene            complement(1345..2244)
                     /locus_tag="PKZ53_14215"
     CDS             complement(1345..2244)
                     /locus_tag="PKZ53_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ41644.1"
                     /translation="MTLNDWKTRGKFFTYRGHQIFYRDEGTGFPVLCIHGFPTASWDW
                     HCVWPELTSRFRVVALDMIGFGFSDKPHAYDYSIMDQATLHETFLEYLKIDRAHIFAH
                     DYGDTVAQELLARCEDRSRQGQSGLEIKSVCFLNGGLFPEVHRARFIQKLLNSPLGSL
                     IGKMITKEKFCRSLSAVFGPKTQPSTEELAAFWTLNSTNQGTQIAHKLIRYIGERKQF
                     RERWVGTLQQTRIPLRVINGLADPVSGAHMVERYRELISNPDVVGLEGIGHYPQVEAP
                     AAVLEGFLSLIGKIERDRSTPST"
BASE COUNT          636 a          592 c          569 g          579 t
ORIGIN      
        1 caaacatcca gccgacacag ccactttcac ctaagttccc accatttttt gaaaacaggt
       61 ggcgcagttc tgaaacggtt cggttgcggt tatcagtggt cgcctggacc ataattgcca
      121 ccccagccgg gccgtatcct tcataggtaa tttcttcgta ggtcaggccc tcgatttcac
      181 cagttccgcg cttaatggcc ttgtcaatcg tatcgttggg aacattttca gccttggcgt
      241 cactgacagc tttgcgcaag cgtgcgtttg atcccggatc accgccgccc attcgggccg
      301 ccacggtgat ttccttcacc attcgggtcg tgatttttcc acgtttggca tcgagcgcac
      361 ccttcttatg tttaatcgta tgccatttac tatgtccaga catgacaatc ctcggaaaat
      421 aatagagata atcgctttac cactgacagt ggttgcaagt gatgaagagg ttttcaggag
      481 aaaactcaag actatagcaa gttgaggtgt gattgaaaac agaaaactct agcacggttg
      541 atggacggcg taaatcgcag ggagataccg ggttactcca ggaaaatccg aaggaaaacc
      601 agtgccagtt gattttgaaa ttgacaaaaa tacagcaagt gttcaggata gcacagcatt
      661 ctggttttaa gttcattcaa ataaaaaagc ttcagtgtct ttctcgattt tctcaggacc
      721 ctcaacttct tctcaagagc caatcaaaac aagcttgtgc accgccttaa catcgcaagg
      781 tgactggcag tctgtggttc gtgtttgcga aaagggtttg ttgatagatg ccgatgccgc
      841 ttcactctga gggaagaaca acaaacaaag aagaaaatag atagagggga taacgaggtt
      901 tttcatgtcg cataggattt taattgttga cgatgaaatg gataccgcac agatgttgcg
      961 ggtgattttg gaatcccgaa attatctctg ttcaattgct tcaaacggga aagatggcct
     1021 tcaactcatt ctcgatgaaa aaccggattt ggtattgatg gattacatga tgcccatcat
     1081 gacaggtgtt gaagccttca agttgctccg acgcattgaa tccagcacca gcaatcggcc
     1141 aacaccggtc atcatgctga cggccaaatc tgacaatgaa gatgaacaga aagagctgct
     1201 tgaccttggg ctgcgtgcct acatcaccaa acccttccgg tatcaaatgt tgttagagac
     1261 tatcggaaaa atcgttgccg aacatcatta aagcgtccgg ctacgatctg taaaccggcc
     1321 ctacctgaaa caatccttga gttttcaggt ggatggggtt gatctgtccc gttcaatttt
     1381 cccaatcagg ctcaaaaaac cttccaacac tgccgccggg gcttcaacct gcggataatg
     1441 cccaattcct tccaatccaa ccacatccgg gtttgaaatg agttcccgat accgctcaac
     1501 catatgggca cctgagaccg ggtcggccag tccgttaatg acgcgcaacg gaattctggt
     1561 ctgttgcagc gttcccaccc agcgttctcg aaactgtttc cgctcaccaa tatacctgat
     1621 caacttgtgg gcaatctgtg tcccttgatt ggttgagttc agcgtccaga aggccgccag
     1681 ttcttcggtt gacggttgtg ttttcggacc aaagacagcc gataagctgc gacagaattt
     1741 ttctttggta atcatttttc caatcaatga tccaagcggt gagttgagga gcttttggat
     1801 gaaccgggcc cgatggactt cggggaacaa gcccccgttc agaaaacata ccgatttaat
     1861 ttccaaccct gattgacctt gccgggaacg atcttcacag cgcgccagca gttcctgagc
     1921 caccgtgtcg ccatagtcgt gggcaaaaat atgcgcccgg tcaatcttga gatactccag
     1981 gaatgtctcg tgcagcgtgg cctgatccat aatggagtag tcataggcgt ggggtttatc
     2041 agaaaaccca aacccgatca tatccagagc gaccacccga aaccgactgg tcaactccgg
     2101 ccaaacgcag tgccagtccc acgatgccgt tggaaagcca tgaatgcaca ggacagggaa
     2161 gcccgtgcct tcatcacgat aaaaaatctg gtggccgcga taggtaaaaa acttcccgcg
     2221 agttttccaa tcatttaatg tcataggcgg tattgcaggg ttcagggttc agggttcgag
     2281 gggaagaaaa ggacaaacgg gacatacagg acgaaaaggg cctgaattcg aaaacccgga
     2341 acccggaacc cggaacccgg aacccggaac ccggaa
//
LOCUS       DAOQDO010000910         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40360_length_2376_cov_3.396812, whole genome shotgun sequence.
ACCESSION   DAOQDO010000910 DAOQDO010000000
VERSION     DAOQDO010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40360_length_2376_cov_3.396812"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKZ53_14220"
     CDS             complement(<1..286)
                     /locus_tag="PKZ53_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41645.1"
                     /translation="MVGEYNPFDRGPFPVGVRTVQAFDPVRNRLFPCEIWYPAAEHHA
                     GQDLVPETQDSFTLPLVNIPRKQSAVRDAVAQTGTFPLVLYSHAGGGHRRA"
     gene            complement(426..1082)
                     /locus_tag="PKZ53_14225"
     CDS             complement(426..1082)
                     /locus_tag="PKZ53_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41646.1"
                     /translation="MKFGPDFHAEHILADGTGIELRHIRPEDAPELRRGFEALSPESR
                     FRRFLTPLQSLSDEMLHYLTEVDGYNHVAIIAVTGSDDLKTERGIGISRFVRLEQEPT
                     VAEVAVTVLDDMQHKGLGRLLLETLARAARERGIEHFRAEVLTSNELVRHALRDAGAV
                     VVADNGDCLTVDIPLNHTKSDDQFENEGLHHESALHRVLREAASSMVDLIRSHRLPML
                     "
     gene            complement(1584..2177)
                     /locus_tag="PKZ53_14230"
     CDS             complement(1584..2177)
                     /locus_tag="PKZ53_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNJ41647.1"
                     /translation="MNTRLFTFSGGKTGDWRITQIETIVGEPLPDAERLTIFASSLVE
                     GPEGTNWRLRGIRSNERYITQPEKDQLVAIQPKLNRPEATCAALIPIRKTADWWALPQ
                     DERRRIFEESSHHVKVGLMYLPAVARRLHHCRDLSENEPFDFLTWFEFAPEHTPAFNE
                     MVAILRATEEWAYVDRECDIRLVRDVEPSPDGITISL"
BASE COUNT          602 a          608 c          585 g          581 t
ORIGIN      
        1 ccgcccgccg atgcccgcca cccgcatggg agtaaagaac cagaggaaaa gtcccagtct
       61 gggcaaccgc gtcacgaacc gctgattgtt ttcgtgggat attgaccaac ggaagggtaa
      121 acgaatcttg cgtttctggt actaaatcct gaccagcgtg gtgttcggct gctggatacc
      181 agatttcaca agggaacagg cggttccgaa ccggatcaaa tgcctggacg gttctgacac
      241 caaccggaaa cggccctctg tcaaagggat tatattcacc aaccaaagag ctttctcttt
      301 caatggaatg cggttgcatc ttctgatttt ccttgagatt tgaaaagata actcatcgct
      361 ggccaaatgg ctcaatcccc agcccgttgt cttcagcccc cagcccgttg tcttcagccc
      421 cagcattaca acatcggcag ccgatgactt cgaatgagat ccaccataga cgaagctgcc
      481 tcccgcaaca cccgatggag tgccgattcg tgatggagtc cctcattttc aaattgatca
      541 tctgatttgg tgtgatttaa gggaatatcc accgtgaggc aatcgccatt gtcagccacc
      601 acaaccgccc cggcatcccg gagggcatgg cggacaagct cattggacgt caaaacttca
      661 gcgcggaaat gttcaatgcc acgttctctg gcggctctgg ccagggtttc caacaataac
      721 cgccctagtc ccttgtgttg catatcatca agaaccgtga cggccacttc agccacagtt
      781 ggctcctgtt ctaaccgaac aaaccgagaa atcccaatcc cccgctcagt tttcaaatca
      841 tctgacccgg taacagcaat gatggcaacg tgattgtacc catctacttc cgtcaaatag
      901 tggagcattt cgtctgaaag gctttgcaat ggagtcagga accgtcgaaa acgggactca
      961 ggtgaaagtg cctcaaaccc acgtcggagt tcaggcgcat cctcggggcg aatatgccgg
     1021 agttcaatcc cggtaccgtc ggcgagaatg tgttcggcat ggaaatctgg accaaacttc
     1081 attgaaatgt cttcctttac ctgatgcctt ttgacggcca gttcctgcct cagtctgaga
     1141 aaacagccag gtagttcaat caaataaact gaatggacga ctggctatga cgacaaactg
     1201 gtgcaagaac agaaagaagc cttgtgataa gtacattgtg gctggtgacg gttaaacaag
     1261 tgtctccagc atggtttccg aatcaggatc gagagcaggg gaagaatgtg tcttggctgg
     1321 tgaatgctgg gttcgcggag ggatattagg aagatagttc atctctggca agcagaatga
     1381 ctggggatat cccggcaagc ggaatccctt tcaaagaaaa tcggatcgtg tctcaccaca
     1441 ctggtggcta attatttctg cgccatcgcc tttcagagcc tgatttcggc tcagggttca
     1501 agcgttcgtt gacttcagtc gagcgaatcg aataactcaa atttcatcaa acacttgaat
     1561 attgaacggt ttcgaaggag ggctcagagg gagatggtaa ttccatctgg cgaaggttcc
     1621 acatcgcgaa ccaatcgaat atcacattcc cggtcaacat aggcccattc ttcagtggcg
     1681 cgaaggatag ccaccatttc attaaaggct ggggtatgct cgggcgcaaa ctcaaaccag
     1741 gtcaaaaaat caaagggctc gttctcactc agatcacgac agtggtggag tcgccgagcc
     1801 acagccggca ggtacatcag tccgactttt acgtgatgtg aactttcctc aaaaattcga
     1861 cggcgttcat cctgcgggag tgcccaccag tcagccgttt tccgaattgg aatcagtgcg
     1921 gcacaagttg cttccggacg attcagtttt ggttgtatcg ccacaagttg atctttttcc
     1981 ggctgagtaa tgtagcgttc attgcttctg attccccgga ggcgccagtt tgttccttct
     2041 ggaccttcaa ccagcgaact ggcgaaaatc gttagccgct cagcatctgg aagaggctct
     2101 ccgacaatgg tttcaatttg agtaatgcgc cagtccccag tttttccgcc tgagaatgtg
     2161 aacagacgag tattcattga agattctccc agttccgcga ttcagagtat ttctgatgag
     2221 tgctttggtt gatgattcaa gagaatccca ctggtatgca ctttattcga cagatgagag
     2281 gtttaaagtc acaggcttat ctcttctttg atcgtctctg cttcaacact atccggtgta
     2341 ccgccaaagg gcaaaggtcg ctcgtaaata gcagca
//
LOCUS       DAOQDO010000911         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40385_length_2376_cov_1.833262, whole genome shotgun sequence.
ACCESSION   DAOQDO010000911 DAOQDO010000000
VERSION     DAOQDO010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40385_length_2376_cov_1.833262"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="PKZ53_14235"
     CDS             complement(<1..2095)
                     /locus_tag="PKZ53_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNJ41648.1"
                     /translation="MKIAEYIHEKVLSPRLKQHGCLVIYDPERRYRELYQRLETDRIK
                     LVDASESSIESREAAMHWLRELGREQSSIDGLVVYVPAPKPLTEEHKQIDPFALYTVC
                     GAVFPMDDDSDDYRNLCLKARKGFEAEIRRVFAENPSPGFAVIDAIGGGIHWPQLRAL
                     LGVESSRDILKALLTPDEKQVTALAATDTWIQEVREFARTTLGLEIKTRAKRWEPITE
                     ELWRFLLFSEFVFDLPETLPESLASVPHAPSVVRPLVDEMCDLLRSDIRTKSRYLERA
                     EAIEQELNLPNACLTIEDLGIRDTFPFEERTFLRQAINGLVKNDLSRTERVISQHGNS
                     IWVGKGENQEPWALLQTALNLIKACGGFEGRLTDHARTLETLIDFYTGHLCEIDRLQR
                     EFEQAVGDILNQYQLMDPVIQQARSAYRRLVEKVQTHFVRNLEASGWPPTGRLSNTDV
                     FDRFVAKHLKERGYKVAYVMVDALRYELGVVLEKQLVDDGPVELHAALAQLPTITPVG
                     MASLLPGARAELTLGCESDKLVPKLAGVPVTNVTQRMEVLSKRYGDRFAEMPLSDFVR
                     SKKKLAPTVDLLVLRSTEIDSYLETNPETALEQVLKTLKLIRTAIHRLRELNFQEAVI
                     VTDHGFFLNAHAEAGDVCTKPNGKWLVAHDRMLLGDGVVDDAHNFVISAEKLGVRGGF
                     ARVAGPRSMAPYSRGH"
BASE COUNT          544 a          634 c          603 g          595 t
ORIGIN      
        1 gatgcccccg gctgtatggc gccatgctac gtgggccagc gactctggca aaaccgccgc
       61 ggacgccaag tttttcagcc gaaatgacaa agttgtgggc atcatccacg acaccatcac
      121 caagcagcat ccggtcgtgg gctaccagcc attttccgtt gggtttggta cagacatccc
      181 cggcttcggc gtgggcattg aggaaaaacc cgtggtcggt gacgattacc gcttcttgaa
      241 aattgagttc gcggagccgg tgaattgcgg tccgaatcag ctttaaggtt ttgagcacct
      301 gctccagggc ggtttccgga ttggtctcca ggtagctgtc aatttcggta ctgcgcaaaa
      361 ccagcagatc taccgtcggg gccagcttct tcttgcttcg cacaaaatcg ctcaggggca
      421 tttcggcaaa acggtcgcca taccgtttgc tgagcacctc catccgctgg gtcacattgg
      481 tcactggaac tcccgccagt tttggcacca gtttgtcgct ttcgcaaccc agggtcagct
      541 cagcccgcgc ccctggcaac aaactggcca ttcccaccgg cgtaatcgtc ggtaattgcg
      601 ccaatgccgc atgcaattcg actggtccat catccaccag ttgtttttca agcaccacac
      661 ccagttcata ccgtaaggcg tccaccatga cataggccac tttataccca cgttctttga
      721 ggtgtttcgc aacaaagcgg tcaaacacgt cggtatttga cagtcgcccg gttggtggcc
      781 agccagaggc ttcaaggttc ctcacaaagt gggtttgaac tttttcaacc aggcggcggt
      841 aggcacttcg ggcctgctgg ataacgggat ccatcaactg gtactgattc aaaatatcgc
      901 ccacggcctg ttcaaactcg cgttgcagcc ggtcaatttc gcacaggtgg cccgtgtaga
      961 aatcaatcag ggtctccaga gttcgggcat ggtcagtgag tcggccttca aagccaccac
     1021 aggctttgat caggttcaaa gccgtctgga gcaaggccca gggttcctga ttttcacctt
     1081 tgccaaccca gattgaattt ccatgctggc tgatgacccg ttccgttcgg gaaagatcgt
     1141 tttttaccag cccgttaatt gcctgacgca gaaacgtgcg ttcttcaaat ggaaaggtgt
     1201 ctcgaatccc aagatcttca atcgtgagac aggcgttggg caagttgagt tcctgttcaa
     1261 tggcttcggc gcgctccagg tacctgcttt tggtgcggat atcgcttcgc agcaggtcac
     1321 acatttcgtc aaccagtggc cggactacac tcggagcgtg cggcaccgac gccagtgatt
     1381 cggggagcgt ttctgggaga tcaaaaacaa attcgctgaa gagcaaaaac cgccagagtt
     1441 cttccgtgat tggttcccac cgtttggccc tggttttgat ttccaaacca agggttgtcc
     1501 gtgcaaactc gcggacttcc tgaatccagg tatcagtcgc cgccagcgct gtcacttgtt
     1561 tttcatccgg tgtcagcaat gccttgagaa tatcgcgcga tgattcaaca cctagaaggg
     1621 cgcgcagttg cggccagtgg attcctccac caatggcatc aataacggca aagccaggag
     1681 acggattttc cgcaaatacc cgccgaattt cagcttcaaa ccctttgcgg gctttcaagc
     1741 agaggtttcg gtaatcgtca ctgtcatcat ccattggaaa cacggccccg cacactgtgt
     1801 agagcgcaaa cggatcaatc tgtttgtgct cttccgtgag tggtttcggt gccgggacat
     1861 agaccaccag cccatcaatt gagctttgtt cccgacccaa ctcacgcagc cagtgcatcg
     1921 ccgcttcgcg gctttcgatg ctgctttccg aggcatcaac cagcttgatt cggtcagtct
     1981 caagtctttg atatagttca cgatagcggc gctctggatc atagatgacc aggcatccat
     2041 gctgtttgag tcgcggtgac aaaacttttt cgtgaatgta ttcggcaatt ttcatagtca
     2101 gaaattcagg ggtaacgaat tgagaagcgt ttcagaagaa taccattgag ggctgagagc
     2161 tgagaaaaat accattttat cgataactta taccaatttg agctgagggt atcgggctaa
     2221 gggctgaggg aaatacaata tttccaatat tttagccttt ccataatgcg aggacttcac
     2281 tccaaaatgg tattagcctt ttcccagcgt gagagtgtca gtccaaaatg gtattaagct
     2341 ttggcataaa tttcctgaga tattgggttt gataag
//
LOCUS       DAOQDO010000912         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40392_length_2375_cov_3.470259, whole genome shotgun sequence.
ACCESSION   DAOQDO010000912 DAOQDO010000000
VERSION     DAOQDO010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40392_length_2375_cov_3.470259"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1406)
                     /locus_tag="PKZ53_14240"
     CDS             complement(177..1406)
                     /locus_tag="PKZ53_14240"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNJ41649.1"
                     /translation="MPKEKAQLTEINGNESSTPDRERPVKDAREARLIPEKMSSAKEL
                     MDLLAQHRGERHIVAIQNFPDPDAIASALAHQMLSAEFGITVDIVYDGFISHQENLAL
                     VQLLHIELLDYDENLDLGQYQASVFVDNQGTTTGLTGKLKEAGVKPLVIVDHHERQGI
                     IEAPFTDLRKVGATATIYTEYLKDSPIAFEKSNPQHVRLATALMHAVRSETNGMIRAR
                     DADFWAAGYLSNFVDQSLLSAILNVKRSKRVIDVIKAALESRIVRDNYSIAGVGYVRY
                     EDRDAIPQAADFLLTEENIHTAVVYGIITKEGEREVIIGSLRTSKVTLNPDTFLKSAL
                     GKDTQGNFYGGGKYEAGGFEIPIGFLSGTYDDEFMRAKWKIYDSMVKRKLFEKIGVEN
                     IQTNAHRSSNQERQNEE"
     gene            1575..2228
                     /locus_tag="PKZ53_14245"
     CDS             1575..2228
                     /locus_tag="PKZ53_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005276713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNJ41650.1"
                     /translation="MEFLSSLIDLILHLNKHLDELVSQYGFWTYGILFLIIFLETGLV
                     VTPFLPGDSLLFAVGALAASSQVLDVNFLFVLLSVAAILGDTVNYGIGHQIGPKVFRQ
                     KNSRLFNKAHLERTHRFYEKYGGKTIIIARFIPIIRTFAPFVAGIGSMTYGRFLVYNV
                     VGGLMWIGLLLYAGYFFGNVPFVKKNFSLVIIAIVILSVLPAIFEIWMQRRGSKNEK"
BASE COUNT          569 a          570 c          532 g          704 t
ORIGIN      
        1 ggcttcgcaa ctcacactcg aaattgaacg agccgagcag tgaaacacaa tgaacaacga
       61 cgaaaataca tagcggttag tggttagtgg ctggaaatca atacgttcca agcaccgatc
      121 actaaccact aaatggtttc ttcattcttc attctcaatt cttcattccc aattctttac
      181 tcttcattct gccgttcctg gtttgaactg cggtgggcgt tggtttggat attctcaacc
      241 ccaatctttt caaagagttt acgcttcacc atcgagtcat aaattttcca cttggcacgc
      301 ataaattcgt cgtcataggt gcccgataga aaaccaatcg ggatctcaaa ccctcccgct
      361 tcgtacttcc ctcccccgta aaaattccct tgtgtatctt ttcccagagc cgatttgaga
      421 aaagtgtctg gattcagagt gacttttgaa gtgcgcaaac tcccgataat cacctcgcgt
      481 tcgccttctt tggtgatgat gccgtatacc actgcggtat ggatattttc ctcagtcagc
      541 aaaaaatcag ccgcttgggg aatcgcatcc cgatcttcat agcggacata cccaacaccg
      601 gcaatgctgt aattatcacg tacaattcga ctttcaagcg cagctttaat cacatcaatg
      661 actcgcttgg aacgcttcac atttaaaatg gcgctcaaca gggattgatc aacaaagttg
      721 gaaaggtagc cagccgccca aaagtcagcg tcacgggccc gaatcatgcc attggtttcg
      781 cttcggaccg cgtgcatgag tgctgttgcc aaccggacat gctgcgggtt tgacttttca
      841 aacgcgatgg gtgaatcctt gagatattcg gtgtaaatgg tggccgtggc cccaactttg
      901 cgcaaatcag taaatggggc ctcgataatg ccctgccgtt cgtggtgatc aacgatcacg
      961 agtggtttga cgccggcctc tttgagttta ccagtcagcc ccgtggtggt gccctggtta
     1021 tcgacaaaaa cactggcctg gtactgccct aaatccaggt tttcgtcata gtccagcaat
     1081 tcaatatgga gtaattgcac cagcgccagg ttttcctgat ggctgataaa tccgtcatag
     1141 acaatatcaa ccgtgattcc aaattcagcc gagagcatct gatgggccag ggcagaggca
     1201 atcgcgtccg gatccgggaa attttggatt gccacaatgt gtcgctcgcc acgatgttgc
     1261 gctaacaggt ccatcagttc tttcgccgaa gacatttttt cgggtatcag gcgtgcctca
     1321 cgggcatctt tgactggccg ctcacgatct ggcgttgaag attcatttcc attgatttca
     1381 gtcagttggg ctttttcctt tggcatatgt cacctgtatt tggtgaaaat tcagagttta
     1441 atttctgaaa atcaaggcag cctgatttac agatcctact ttctggttgg aaagagagac
     1501 tttctttctg atcagaggat gctagaatgc ccgactttta tccccgcccc ctgtcaggac
     1561 acgtcatcaa gcctatggaa ttcctctctt cgttaattga tctgatttta cacttaaaca
     1621 agcacctgga cgaactggta agccagtatg gattctggac ctatggcatt ttgtttctga
     1681 ttatttttct ggaaacaggg ctggtggtca caccattttt gccaggtgat tcactcttat
     1741 ttgcagttgg agcgctggcg gcctccagcc aggtactgga tgtgaatttc ctgtttgtgc
     1801 tgctcagtgt cgctgcaatt ttgggtgata ccgtaaatta tggaatcggt caccagattg
     1861 gtcccaaagt gtttcgccag aaaaattcac gcctgtttaa caaagctcac cttgaacgaa
     1921 cccatcggtt ttatgaaaaa tatgggggca agaccattat tatagcgcgt tttatcccga
     1981 ttatccgcac ctttgcccca ttcgtcgccg gcattggcag catgacctat ggccggtttc
     2041 tggtgtacaa cgtggtgggc ggtctgatgt ggattggctt attgctctat gcaggctatt
     2101 tctttgggaa tgttccgttt gtgaagaaaa acttctcgct ggtcatcatt gcgattgtta
     2161 ttttgtcagt attaccagcc atttttgaaa tctggatgca gcggcgtggg agcaagaatg
     2221 aaaaatgaaa aaaccattta gtgtggttgg tacctggtac ctggtacctg gtgcttagta
     2281 cttagtgctt tgaaaccaat actttcaaag aaccaagaat cagtttttct tcattcttca
     2341 ttcttcattc ttcattcttc attcttcatt cttca
//
LOCUS       DAOQDO010000913         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40444_length_2374_cov_2.427771, whole genome shotgun sequence.
ACCESSION   DAOQDO010000913 DAOQDO010000000
VERSION     DAOQDO010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40444_length_2374_cov_2.427771"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..2158
                     /locus_tag="PKZ53_14250"
     CDS             227..2158
                     /locus_tag="PKZ53_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNJ41651.1"
                     /translation="MADLSQLTPPQILRTVFGFETYRTGQEAVISQLLTGHSALAVFP
                     TGGGKSLCYQLPALCLPGLTLVVSPLLALMKDQVDFLLSKGVAAARLDSSLDAEQYNQ
                     VLHQLGQRSLKLLYVSPERCTNQNFRTLIAHHRISLLAVDEAHCISQWGPNFRPDYLQ
                     IAQFARDVRVERTLALTATATPQVVEDIAVNFGISPDHITNTGIYRPNLTLRFIPVEN
                     RARDASLFESDFSGPTIVYVTQRSTAEQIANRLAVRGIPARPYHAGMNTEEREEIQNW
                     FLASEQATVVATIAFGMGIDKSNIRAVYHFNLPKGIEGYIQEIGRAGRDGKPSLCQSF
                     LSADDVPVLANFAYGSTPTREAIEGVVTEIFSGSTELELNLYDLSRRFDIREPTLKTL
                     LAHLQLDGYLKSRMPVYLLYEFQLQHSVEKTVQLVAPEMSERVRKFIAHASKGITWYK
                     IDFGGTHKHLEPHRHRAEEILNHLKERNLAVVKNSGVRHRFSVLQTPNKLAALVKASW
                     ERFTAQERMEIERLEEMLRLVQSDQCHWNALARYFGEVRTTACGHCSFCELKKAITLK
                     PRSVSHTVPVQTIRQTFQLDPEVFEHPRVLARFLCGISSPRLTQRKLTKHENFGVFSE
                     FDFHKVLAHCENVVGATAR"
     gene            complement(2122..>2374)
                     /locus_tag="PKZ53_14255"
     CDS             complement(2122..>2374)
                     /locus_tag="PKZ53_14255"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ41652.1"
                     /translation="YATYSGANQRTTRDLLAEFAAVRASTIALFQSLDADALLRSGTA
                     NGNVMSVRAAAYHIAGHELRHLNIIRERYLAVAPTTFSQ"
BASE COUNT          584 a          625 c          604 g          561 t
ORIGIN      
        1 gaaccaagaa ccaagaacca agaaccaaga accaagaacc aggaaccaag aaccaggaac
       61 caagaaccaa gaaccaagaa ccaggaacca aacatcagga acattcttca ttcttcattc
      121 ttcattcttc attcttgccc ttgcccggca cgtgagttgc tcaaaggagg ttgcaattta
      181 aaagaaccga tacccaggaa cattctaaac gattagggat attcttatgg ctgacctttc
      241 tcaactcaca ccccctcaaa tcctgcgcac tgtgtttgga ttcgagacct accgtaccgg
      301 tcaggaagcg gttatttcac aacttcttac aggtcattcc gcgctggcgg tttttccgac
      361 tggcggtggg aagtcattgt gttatcaatt gccggcgttg tgcctgccgg gattgacgct
      421 ggtggtgtca ccgttgctgg cgctgatgaa agaccaggtt gattttcttt tgtcaaaggg
      481 tgttgcggcg gcgcgcctcg attcaagcct ggatgctgaa caatataatc aggtactaca
      541 tcagcttgga cagcgttcgt taaaactgct ctatgtttcc ccggaacggt gcaccaatca
      601 gaactttcgc accttaattg ctcatcaccg gatttcgctt ctagctgtgg atgaagccca
      661 ctgtatttcc caatggggac cgaattttcg ccccgattat ctgcaaatag cccagtttgc
      721 gcgtgatgtc agagttgagc gaaccctggc cctgactgcg acggcaacac ctcaggtggt
      781 tgaggatatt gcggtgaatt ttggaatctc gcccgaccat atcaccaaca ccgggattta
      841 tcgtcctaat ttaacgctgc ggtttattcc ggttgagaat cgtgcccgcg acgccagttt
      901 gtttgaaagc gacttttcgg gaccgacgat tgtctacgtc acccagcggt caacagcgga
      961 acagattgcc aaccgactgg ccgtcagggg aattccggcc cggccctacc atgcgggaat
     1021 gaataccgaa gagcgcgagg aaatccaaaa ctggtttctc gccagcgaac aggccaccgt
     1081 ggtcgccact attgcctttg gaatgggcat tgacaagtcc aatatccgcg cggtgtatca
     1141 ttttaatttg cccaaaggaa tcgaaggcta cattcaggaa attggacgtg ccggacgcga
     1201 tggaaagccc tcgctctgcc agagttttct gtcggctgat gatgtgccgg tgctggctaa
     1261 ctttgcttat ggcagcacgc cgacccgaga agcgattgaa ggcgtggtga ccgaaatctt
     1321 ttccggttca accgaactgg aattaaattt gtatgatctt tcacgccgct ttgacatccg
     1381 tgaaccaacg ctcaaaacac tgcttgccca cctgcaactg gatggctatc tcaaaagccg
     1441 gatgccggtc tatctgctct atgaatttca acttcagcat tcggttgaga aaacagttca
     1501 actcgtagca cccgaaatga gcgaacgggt tcgcaaattt attgcccacg ccagcaaagg
     1561 cattacctgg tataagatag atttcggagg cactcacaag cacctggaac cacatcgtca
     1621 ccgagccgag gaaattttga accacctcaa ggagcgcaat ctggcggtgg tcaaaaattc
     1681 gggcgttcga catcggtttt cagtccttca aactccgaac aagctggctg cactcgtcaa
     1741 agcgtcatgg gagcggttta cagcacagga gcgaatggaa atcgaacggt tggaagaaat
     1801 gttacgcctg gtgcagtctg atcaatgtca ctggaacgcg ctggcccggt attttggtga
     1861 agttcgaacg acggcctgtg gccactgttc gttctgtgag ttgaaaaaag caatcacgct
     1921 caagccccgg tccgtgtctc acaccgtgcc ggttcagacc atccggcaga cattccagct
     1981 tgatccggaa gtatttgagc atccacgggt tctggcacgc ttcctgtgcg gaatctcttc
     2041 gccacgtttg acgcagagaa aactgaccaa acacgaaaac tttggtgtgt tttcggaatt
     2101 cgattttcac aaagtcctgg ctcactgtga aaatgtggtc ggggccaccg ccagatagcg
     2161 ttcccggata atgttgagat gtcggagttc atgcccggca atgtggtaag ccgccgcccg
     2221 gacgctcatc acattgccgt tggccgttcc tgaccggagc aacgcgtcag catccagact
     2281 ctgaaacaag gcaatggtcg aggctcgcac cgcggcaaat tcagccagca ggtcacgggt
     2341 cgttcgttga ttggcgccag agtaagtggc gtac
//
LOCUS       DAOQDO010000914         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40551_length_2371_cov_2.545337, whole genome shotgun sequence.
ACCESSION   DAOQDO010000914 DAOQDO010000000
VERSION     DAOQDO010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40551_length_2371_cov_2.545337"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1560
                     /locus_tag="PKZ53_14260"
     CDS             58..1560
                     /locus_tag="PKZ53_14260"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ41653.1"
                     /translation="MTFIARIIRFVISVVLIGLTGNLLTIARQMPPETAKVPVPAELA
                     TARSRVVDRIRRREAPSFAIGVVKNGKVIWEEGLGWADRERKIAATVESRYAVASVSK
                     SITATGTLALVGKGQFKLDQSVNSILGAGWAPMIGYPSQDVLISHLLAHTSGIPHLWH
                     YQYPDVPASLVGRDALIQKYAFQAAPPGQHYVYTNLGYGVLAKVIEKAGNAPYQRVME
                     QSVFQPLGMQRTTTDAWLGDKNTVRGYGGDGKVLPYRFRLGPDGGAGFFSTAHDLTRY
                     VLFHLGAMTPPAHLRGASISTELANLPVEKAYYRGWGVVKLPTSTMLISDGQMAGGTA
                     VVVLVPEQHIGVVVLSNATGSPSAEVAVDILNALIPEFGAQFGAAIGVLEQKLTAPGQ
                     LPTGCFKGSLKSVTDDVPVSLDFRNPELPVFELGGNKFILQGISWERGAMQATVTGSL
                     AFARGRDRTHNLVLTMWLHNGELQGIAQEDFTDDRSRSGVPYRLTLKPVP"
     gene            1571..1888
                     /locus_tag="PKZ53_14265"
     CDS             1571..1888
                     /locus_tag="PKZ53_14265"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNJ41654.1"
                     /translation="MKPRRIILIRHGESEGNADREMYQTTPDCKLNLTDLGREQAREA
                     ENTIKSIIGDSSVYAYISPYYRTRQTFEEFESLRNPKNCQIVVMEKADDKYQLLTELA
                     RRR"
     gene            1953..>2371
                     /locus_tag="PKZ53_14270"
     CDS             1953..>2371
                     /locus_tag="PKZ53_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41655.1"
                     /translation="MKSISIQTLNLQVNQRHDPGRLLSVKLWCVLLTLVILFTSMAFP
                     VLAEPQANKCDLPQSRPEAEGLDRATLDAVLKQVRAAGTDAFVILRNGNVVVEEYNGR
                     PERILDQSVTKSITSLAVGTAIDHKFIKGPDQLASDF"
BASE COUNT          576 a          596 c          641 g          558 t
ORIGIN      
        1 cgagcccctg atcccaaaat gatataatca gatttactcc aaatcgtgag agggtacatg
       61 accttcatag ctcggatcat ccgttttgtg ataagtgtgg tattgatcgg cctgactgga
      121 aacttactca ctatcgcaag gcaaatgccg cctgaaacgg caaaagtccc tgtccccgcc
      181 gaactcgcca ccgcccgtag ccgtgtggtg gatcgaattc gccgacgaga agcgccgtcc
      241 tttgccattg gtgttgtaaa aaacggaaaa gttatctggg aagaagggct tggctgggct
      301 gaccgggagc gaaagattgc ggcgacggtg gaaagtcgct acgcggtggc ttcggtctca
      361 aaatcaatca ctgcgaccgg gacgctggca ctggtcggga aaggccagtt caaactcgat
      421 caatcagtca attcgattct aggggctggt tgggcgccga tgattgggta cccatcgcag
      481 gacgtgttga tttcccattt actggcccat acttcgggga ttccacacct ctggcattat
      541 caataccctg acgttccagc cagtcttgtc gggcgtgatg cgctcattca gaagtatgct
      601 tttcaggctg caccaccggg gcaacactac gtttacacca atttaggcta tggcgtgctg
      661 gcaaaagtca ttgaaaaagc agggaatgca ccatatcagc gggtgatgga acagtcagtc
      721 tttcaacccc ttggaatgca gcgaacaaca actgatgcct ggctcggaga caaaaacacc
      781 gtgcgtggct atggcggaga cgggaaagtg ttgccatacc ggtttcgact gggaccggat
      841 ggcggtgctg gatttttctc gacggcgcac gacctgaccc ggtacgtatt gtttcatctc
      901 ggcgcgatga ctccaccagc gcatttgcgt ggggcttcga tttccacgga gctggccaat
      961 ttgccggtgg aaaaagccta ctatcgtggc tggggcgtgg tgaagttgcc gacttccacc
     1021 atgctgatta gcgatgggca aatggctgga ggcacagccg ttgtggtcct ggtgccggag
     1081 cagcatatcg gggtggttgt gctttcgaat gccaccggga gtccttcggc tgaagttgcg
     1141 gttgatattc ttaatgcact tattcctgaa ttcggtgctc agtttggcgc tgcaattggt
     1201 gtgttagagc aaaaattgac cgctcctggt cagcttccaa ccgggtgttt caaaggatcg
     1261 ctcaaaagtg taaccgatga cgtgccggtc agcctggatt ttcgcaaccc ggaactcccg
     1321 gtatttgagc ttggaggcaa caaatttata ttgcagggta tttcctggga gcgcggcgca
     1381 atgcaggcaa cagtgactgg ttcgcttgcc tttgcgcgag ggcgtgaccg aacccataat
     1441 ctggttttaa cgatgtggct gcacaacgga gagctccagg gaattgccca ggaagatttc
     1501 actgatgacc ggtcacgaag cggcgtcccc tatcgcctga cgttaaaacc tgttccataa
     1561 ccagtcagtc atgaaaccca gacgaatcat cctcatccga cacggcgagt cagaaggcaa
     1621 tgctgaccgt gaaatgtatc aaaccacacc tgactgtaag ctcaatctca ctgacctcgg
     1681 acgtgagcag gcacgtgaag ctgaaaatac aatcaaatca atcatcggcg acagttcggt
     1741 gtatgcctac atttcgccct attaccgaac ccgccagacg ttcgaagagt ttgaatctct
     1801 gcgcaatcca aaaaattgcc agattgtcgt catggaaaag gctgacgata aataccagct
     1861 tctcaccgaa ctggcccgaa gaagataggc attgcccggt caattcctgt ctgggaaaca
     1921 agcgatagat tacaaaatgc aggtgaccta cgatgaaaag tatttcaatc cagacactga
     1981 atcttcaagt aaatcaacgt cacgatccag gccggttgtt gtctgtcaaa ctgtggtgtg
     2041 tgctgctcac actggtaatt cttttcacgt caatggcgtt ccctgttctg gcagaacccc
     2101 aggcaaataa atgtgatctg ccgcagagtc gccctgaggc tgaaggcttg gaccgggcaa
     2161 cgctcgatgc tgtgttaaag caggttcgag ctgccggcac cgatgcgttt gtgatcctgc
     2221 gaaacgggaa tgttgtggtt gaggaataca atggtcgtcc cgagcgcata ctggaccaat
     2281 cagtaaccaa atcaattacc tcactggctg tcgggaccgc cattgatcat aagttcatca
     2341 aggggccaga ccagctcgct agtgattttt t
//
LOCUS       DAOQDO010000915         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40567_length_2370_cov_3.763283, whole genome shotgun sequence.
ACCESSION   DAOQDO010000915 DAOQDO010000000
VERSION     DAOQDO010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40567_length_2370_cov_3.763283"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PKZ53_14275"
     CDS             <1..1374
                     /locus_tag="PKZ53_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41656.1"
                     /translation="VVVTITGGLEPYDVTLTNGGGFMSGNSPLTFLVNPAVTTTYTVQ
                     EGNDANGRSYMVVGSATVTVGAANEPTVAQAGPNQGLCSPGPVTLAANVPTVGSGSWS
                     IVSGPDTSLLQFGDVTLNTSTFTATSTGVYVLRWTITNGSCGTSTDDVQVTILNGCNS
                     ALLVADTNNHRIQRFDGSDWFLVGPGTMGSAPGQFRLPEAVTADISGQTIYVADTGNN
                     RIQWSTDGGANWMIFATLGSGLNQVRSPQGVALDLDGNLYVSDTGNSRVLRFENGLPG
                     LAIVIASTGTGSGQVGSPRGLVVAPDFRLFVADEINSRIVRIVNANTVLTSTSGTVLA
                     SKGVGLNKVQNPQGVTIDPTTGTLYIADTGNSRILRFPNASPNNASALALIGSALGQV
                     NRAEGITVCFFNTGPFTEGAFLVVGDTFNNRIQGRFIPTGAWSLVGSPNNVGSAIGQF
                     RNPSKIW"
     gene            complement(1473..2252)
                     /locus_tag="PKZ53_14280"
     CDS             complement(1473..2252)
                     /locus_tag="PKZ53_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41657.1"
                     /translation="MALPSPIIEVSPITSEPEASVPGYAITRRMFCWLPLAMTASSAV
                     AKGNPLQEPKKVDSPATQPDVLTWAEFLQQAEGFEGVKLAGDQVDPYVYRLASLAAQV
                     QGVPAARVFPFATLQPPVYFGPSHKGAGLIIIEWKLAPGAVLPPHNHPNYSICTLGLE
                     GEARMSNYEVVGTAPEFSSSKSFQVRKTNTQWMRPGVVNTLSPHRDNIHTFQAGKDGA
                     RGIDIGTLHGTDRGFSFLKLTEKPTDPARDIFEAQWIGTKP"
BASE COUNT          547 a          613 c          641 g          569 t
ORIGIN      
        1 gtggttgtca ccatcacagg cggactcgaa ccatatgacg tcacgctcac caatggcggt
       61 gggtttatgt cagggaatag tccattaacc ttcctggtaa accccgcagt cacaacaact
      121 tataccgtgc aggagggtaa tgacgccaat ggtcgctctt acatggtggt tggcagcgcc
      181 acagtgacgg ttggcgccgc caatgagccg acagttgctc aggcaggtcc caaccagggg
      241 ttatgtagcc caggacctgt cacccttgcg gccaatgttc ccaccgtagg ctcaggaagc
      301 tggtcaatcg tcagtggacc ggacacctcc ctgctccagt tcggagatgt gacgcttaac
      361 acttcaacct ttacggcaac ctcaaccggt gtctatgttc tgaggtggac cattaccaat
      421 ggaagttgtg ggacttcaac cgatgatgtg caggtaacaa tcctgaatgg ttgtaattca
      481 gccttgcttg tggccgatac caacaaccat cggattcaac gatttgatgg ttctgactgg
      541 tttctggtgg gaccaggaac aatggggtca gctccaggtc agttccgcct gcccgaagcc
      601 gttacggctg acatttcagg ccaaactatc tatgtggcag atactggaaa caatcgcatt
      661 cagtggtcaa ctgacggtgg ggcaaactgg atgatttttg caacgctggg ctcggggctc
      721 aatcaggttc ggtcgccaca aggggtggcc ctggatctgg atggaaatct ctatgtttca
      781 gacactggaa atagtcgtgt cctgcgcttt gagaatggcc tgcctggact ggcaattgtc
      841 attgcctcga ctggaactgg cagtggccag gttggtagcc ctcggggact ggtcgtggcc
      901 ccagattttc gcctttttgt cgccgatgag atcaacagcc ggattgtgcg cattgtgaat
      961 gccaacacag tgctcaccag caccagcggc acggttctgg cgtcgaaagg cgttgggtta
     1021 aacaaagtac agaacccgca aggtgtgacg attgatccaa ccaccgggac actctatatc
     1081 gcggacacag gaaactcgcg aatcttgcgc ttccccaatg ccagtccaaa taatgcttcg
     1141 gcgctcgccc tcattgggtc agccctgggt caggtcaatc gggctgaagg gatcacggtt
     1201 tgctttttca acacaggtcc cttcactgaa ggagcctttc tggtggttgg cgacacgttc
     1261 aacaatcgca ttcagggacg atttatccca actggcgcct ggtcgctggt gggatcgcca
     1321 aacaatgtcg gaagtgcgat tggccagttt cgcaatccat ccaaaatctg gtaagggtgg
     1381 agcatgaagg aattgagaat gaccaataac gaatgtgagt aaggtctttt tttcacctta
     1441 tcattctctc attttgtcag cctctcaccc ggtcagggtt tggttccaat ccattgagcc
     1501 tcaaaaatat cacgggccgg atcggtgggt ttttcagtca gcttcaaaaa cgaaaacccg
     1561 cgatccgtac cgtgcaatgt cccaatatca atcccccgcg ccccatcttt cccggcctgg
     1621 aaggtatgaa tgttgtcacg gtgtggtgag agcgtgttga ccacccctgg acgcatccac
     1681 tgagtgttgg tttttcgaac ctggaagctt ttgctggatg aaaattcagg cgcggtaccc
     1741 acgacttcat agtttgacat ccgggcttcg ccttcgaggc caagcgtgca aatactgtag
     1801 tttggatggt tatgcggtgg cagaaccgca ccaggagcca gtttccattc aataatgatc
     1861 aacccggcgc ccttgtgtga agggccgaaa tacactggtg gttggagcgt ggcaaatgga
     1921 aacaccctgg cggcgggaac cccctgcacc tgtgcagcca gcgaagccaa ccgatacacg
     1981 tacgggtcaa cctgatcgcc agcgagtttg actccctcga agccttcggc ctgttgcaaa
     2041 aattccgccc acgtcaaaac atcaggttga gtcgccggcg aatcaacttt tttgggttct
     2101 tgaagtggat ttcctttggc aacggctgaa cttgccgtca tggcgagggg aagccagcaa
     2161 aacatccgcc gggtgatggc ataccctgga actgatgctt cgggttctga tgtgatcgga
     2221 gaaacttcaa ttattggtga tggtaaagcc atgtcgttcc tgaaacctcc tgcagcacgt
     2281 agttgacggc ggagtttagg aacgtgaaac cagagaatgc ttcttgaaaa tcactttttt
     2341 cgaaatgtat caggcgaaca cccgacttta
//
LOCUS       DAOQDO010000916         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40573_length_2370_cov_3.221166, whole genome shotgun sequence.
ACCESSION   DAOQDO010000916 DAOQDO010000000
VERSION     DAOQDO010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40573_length_2370_cov_3.221166"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1062)
                     /locus_tag="PKZ53_14285"
     CDS             complement(10..1062)
                     /locus_tag="PKZ53_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNJ41658.1"
                     /translation="MFQKFFPWFGLDLAIDLGTANTLIFARGQGLVLNEPSVITLHEV
                     TGKVEATGKSARAMLARTPHLLRTIQPLKDGVVANLTATELMLTDFLRKVRKNNTGIS
                     GVRALIGVTSDATPVESRAIVHAAMRAGCSSVQLVSEPLAAAIGAGLAVAEPMGRMVV
                     DTGGGVTDIAVISLSDIVLAQTLRMGGSQIDTAIMQHVRHQHLLLIGEPTAEQVKIKI
                     GSAMPLSAPRTMEVCGRSITDGLPRTVKLTDSEIRSAIAPVVNQIVSAVRKALDKVPP
                     EVAADLTEQGIVLTGGGAKLIGLSDLIARETRLPVQLAPDPMLSVVYGVSQMLEVAAQ
                     VNGGPSLPWTLRPSES"
     gene            1543..2331
                     /locus_tag="PKZ53_14290"
     CDS             1543..2331
                     /locus_tag="PKZ53_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNJ41659.1"
                     /translation="MSGELVSYRTQNGVAILEMNDPPANTYTYEMNRELDEAILRARF
                     DDDVHVIVLRGAGEKFFSAGANIKMLSEVTPQFKYYFCLHANETLNRLEQTPKLVIAA
                     LNGHTVGGGLEIAMAADLRIAKRGGGKVGLPEVTLGVLPGTGGTQRLARLIGKSRSIE
                     LMITGRLLEFEEAKEIGLVNEIFDAENFFDKVLEYAGQFVPPNKASKAVGRIKRSVQS
                     GAEVPFESGLTLERELQQQLFQSEDAKEGLNAYVNKRTPKFSGK"
BASE COUNT          584 a          604 c          621 g          561 t
ORIGIN      
        1 ggtgtggggt tagctttcgc ttggacgaag tgtccagggg agtgacgggc ctccattgac
       61 ctgagccgca acttcaagca tttgactgac accatatacc accgagagca tcgggtctgg
      121 ggccagttga actggcaacc gggtttcacg tgcaatgagg tcagagagcc caatcaactt
      181 ggcgccccca ccggtgagca caatgccttg ttcagtcaaa tcggcggcaa cctcgggtgg
      241 caccttatca agtgctttgc gaacggcgct cacaatctgg ttgaccaccg gggcaattgc
      301 actccggatt tcgctatcgg ttaattttac cgttcggggc agaccatcag tgatgcttcg
      361 accgcacact tccatcgtgc gtggggctga cagcggcatg gccgaaccaa ttttgatttt
      421 gacctgctcg gcagttggct caccaatcaa caacagatgc tggtgacgaa cgtgctgcat
      481 aatggcggtg tcaatctgag acccgcccat ccgcaaggtc tgtgccagga caatgtccga
      541 gaggctaatc acggcaatgt cagtcactcc accgccagtg tcaaccacca tccggcccat
      601 tggttctgcc acggccagtc cagctccgat ggctgccgcc agcggttctg agaccagttg
      661 cacgctggaa catccagctc gcatcgcggc atgaacaatc gcccggcttt caaccggggt
      721 agcgtcactg gtgaccccaa tcagggcgcg aacaccactg atgccagtat tgttttttcg
      781 aactttgcgg aggaaatcag tcaacatcaa ctcagtcgcc gttaaattgg ccaccacgcc
      841 atctttgagc ggttgaatcg tccgtagcaa atgcggtgtc cgggccagca tggcccgggc
      901 cgatttgccg gtcgcttcaa cttttccagt tacttcgtga agggtgataa cggaaggctc
      961 gttgagtact aatccctggc cacgtgcaaa aatcagagta ttggcggttc ccaaatcaat
     1021 cgcgagatcc agaccaaacc atgggaaaaa cttttgaaac atagctgaat tatgaggacg
     1081 aagagatcga gatgcgctcc actgtgcttc agtcaatcct aacaggtact caagacgtca
     1141 agcggttgcg tcaccttgta tctgaagcag taaacgtgtg agcttggtat aagataaagg
     1201 gcgaatgtag cagaaaaatt tcattgctgg aagcctcttt ccggatccaa gaagctgagg
     1261 tgtgcggttc gagcctgttt cttaacccag gatctcttta tttttcaact gcatgggttc
     1321 aggaattgaa agtaaaaaat agatgggcaa agccgccgaa tcgggtcaag aaagccatac
     1381 ttttgacgca cagtgttaca aaactgtgat tttgtgaggc aaagatgcca gtcggtcttc
     1441 tctttttttg ggtctttgaa gtaggatgtg cccgcttttc cagtgtaatt ttctaataca
     1501 catcatcttc aaaatttttg cacttgtttt caaggagtcc ctatgtctgg agaacttgtt
     1561 tcataccgca ctcagaacgg cgtcgcgatt ctggaaatga atgatccgcc ggccaatact
     1621 tacacttatg aaatgaaccg cgaacttgac gaagccattc tccgagcccg gttcgatgat
     1681 gacgttcacg tcattgtgct gcgcggagct ggtgagaagt ttttctcagc cggtgccaac
     1741 atcaaaatgc tgtctgaagt gacaccacag tttaaatact acttttgtct gcatgccaat
     1801 gaaactctca accggttaga gcagaccccc aagctggtga ttgccgcact caacggccac
     1861 accgttggcg gtgggttgga aatcgccatg gcggctgatt tgcgcattgc caaacgcggt
     1921 ggtggcaaag ttggtcttcc ggaagtcacc ctcggggtat tgcccggaac cggcggcacc
     1981 cagcggctgg cacgcttgat tggcaagtca cggtcaattg aattgatgat taccggtcgc
     2041 ctgctcgaat ttgaagaagc caaggaaatt ggtctggtca atgagatttt cgatgctgaa
     2101 aacttctttg ataaagtcct cgaatatgcc ggtcagtttg tgccacccaa caaagccagc
     2161 aaagccgtcg ggcgcattaa acggtcagtg caatccggag ccgaagttcc atttgaatcc
     2221 gggttgaccc tggaacgcga actgcagcag cagcttttcc aaagcgaaga cgccaaagaa
     2281 gggctcaatg cttatgtgaa caagcgcaca ccaaaattca gtggcaaata agtgttaggg
     2341 ctgaaggact cgcaagctcg gggctgaaga
//
LOCUS       DAOQDO010000917         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40614_length_2369_cov_2.679775, whole genome shotgun sequence.
ACCESSION   DAOQDO010000917 DAOQDO010000000
VERSION     DAOQDO010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40614_length_2369_cov_2.679775"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..945)
                     /locus_tag="PKZ53_14295"
     CDS             complement(94..945)
                     /locus_tag="PKZ53_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ41660.1"
                     /translation="MTVQLGDFQLDIVSDGTFYLDGGAMFGVVPRVVWERVMPPDDRH
                     RIRLGTNTLLVRTPHETILIDTGIGEKWSPKHLEMYGIQHETTVPKSLAALGLSVDDI
                     TLVIDTHLHFDHAGGNTFVDADGTLKPTFPKARYVVQRQEYLHACSPHERDRASYMPE
                     NWEPLVATGQLEFSDGEQEISPGITVVPIRGHNDFTQCVRIESAGNIAFYWADTIPTT
                     AHIPFAWVMGYDLYPVELLEHKKRLIPQAAREGWLNVFEHDQDTPMAYIIEEKGKYRA
                     KALKNEE"
     gene            1190..2095
                     /locus_tag="PKZ53_14300"
     CDS             1190..2095
                     /locus_tag="PKZ53_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNJ41661.1"
                     /translation="MDTLIKLAEVIREMLNFPLIQFGKTPVTLWTLLYLGMLILALVT
                     FSNWLKRWIVKRALARPSIHIGVREAFGSIIRYFTLVIGLMMIMQTAGIDLTSILTLA
                     GALGIGIGLGLQNITNNFVSGLIILFERPIKVGDRIEVGNITGDVVAISPRATTVITN
                     DNIAMIIPNSEFISSRVINWSYRDQRVRFNFKLPVSYTVKPEAVRKVLLEVAAEHPGV
                     LKDRRSDVLIHEFGDSGIIYILRVWSSEYIGNPGVLRSELFYSVVRKFEEQGIVIPFP
                     QRDIHIRSGAVELKAREELRMKNEE"
     gene            2113..>2369
                     /locus_tag="PKZ53_14305"
     CDS             2113..>2369
                     /locus_tag="PKZ53_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41662.1"
                     /translation="MKKRPGTWFLVLGFWFLVLGSWFLVLGFWFLVLGSWFLVLGFWF
                     LVFGSWKVLVPKHQAPSTKHQAPKLHSSSLFERPEIGHQPH"
BASE COUNT          567 a          550 c          587 g          665 t
ORIGIN      
        1 tgggttgatg gcgttcgcat ggctcataag ttgaataatt ttgggtaatg acaagacccg
       61 gaacccggaa cccggaattg gtttcttcat tcttcattct tcattcttca gggctttcgc
      121 ccggtatttc cctttttcct caataatata ggccattggt gtatcttgat catgctcaaa
      181 cacgttgagc cagccttcgc gagctgcctg tgggataagc cgttttttgt gctccaggag
      241 ttccaccggg tacaggtcat agcccatcac ccaggcaaaa ggaatatggg ctgtggttgg
      301 aatggtgtcg gcccagtaaa aggcgatgtt cccggcagat tcgatccgca cacattgagt
      361 gaagtcatta tgcccacgga taggaaccac ggtaatgcct ggagagattt cctgctcacc
      421 atctgaaaat tccaactgtc cggttgccac caggggttcc caattttccg gcatatagct
      481 ggcccggtcg cgttcgtgcg gggaacaggc gtgcaggtat tcttgccgtt gcaccacata
      541 gcgcgccttt ggaaatgtcg gtttgagtgt tccatctgcg tccacaaagg tgttcccgcc
      601 agcgtggtca aaatggagat gggtgtcaat caccagggtg atgtcatcaa ccgaaaggcc
      661 gagggccgcc agtgatttcg gcaccgtggt ttcatgctgg atcccgtaca tttccagatg
      721 tttcggcgac catttttcac caattccagt gtcaatcaga attgtttcgt ggggagttcg
      781 caccagtaag gtatttgtcc ccaaccgaat acggtgccgg tcatccgggg gcattacccg
      841 ctcccaaaca acacgaggta cgacaccaaa catcgcacca ccatccaggt aaaaggttcc
      901 atctgacaca atatcaagtt gaaagtcacc aagttgaaca gtcatatcac accagttttt
      961 cgtcagttgt cagttgtcag tagttcgcta agcttattcg attgaatcac ttaattggtt
     1021 tctaataggg tatttaattg caaattggtg ttacttaaca tttattgaat gaaaggaaag
     1081 aattttttat tggtatcatt attccacctg gtagtcaaac tattgtagaa tataggttta
     1141 tttactactg acgactgacg actgacttat ctcatcgaaa ggaaaagcaa tggatacatt
     1201 gatcaaactg gctgaggtga tccgtgaaat gttgaacttt ccgctcattc agtttggaaa
     1261 aaccccggtg acactctgga cgctgctcta tttgggaatg ttgatcctgg cactggtcac
     1321 cttttcaaac tggctgaaac gctggattgt caaacgcgct ctggctcgac catcaatcca
     1381 cattggcgtc cgtgaggctt ttgggtcaat tatccgctat ttcactctgg tgattggcct
     1441 gatgatgatt atgcaaaccg ctggaattga cctgacgtca attctgacgc tggccggtgc
     1501 gctgggaatc ggtatcggtc tgggcttgca aaacatcacc aataactttg tcagcggctt
     1561 gatcattttg tttgagcgac ctatcaaagt cggagaccgc attgaagtcg gcaatatcac
     1621 cggcgacgtg gtggcaattt cgccacgggc gacaaccgtg attaccaacg acaacattgc
     1681 catgattatt cccaattcgg aatttatctc ctcgcgggtg atcaactgga gctatcgtga
     1741 ccagcgggtt cggttcaatt ttaagctccc ggtgtcgtat accgtcaaac ccgaggcggt
     1801 ccggaaagta ctgctcgaag tcgccgccga acatcccggc gtgctcaaag accgccgatc
     1861 tgatgtgctc attcatgaat ttggcgacag tggaatcatc tatatcctgc gggtctggtc
     1921 gagtgaatac atcggcaacc caggggtgct acgcagtgaa ttgttttatt ccgtagtgcg
     1981 aaaatttgaa gagcaaggca tcgttatccc cttcccccaa cgcgacatcc atatccgaag
     2041 cggtgcagtc gagctgaaag ccagggaaga attgagaatg aagaatgaag aatgaagaat
     2101 gaagaatgaa gaatgaagaa acggcctggt acctggttct tggttcttgg tttttggttt
     2161 ttggtgcttg gttcttggtt tttggttctt ggtttttggt tcttggtgct tggttcttgg
     2221 tttttggttc ttggtttttg gttcttggtt tttggttctt ggaaagtatt ggttccaaag
     2281 caccaagcac caagcaccaa gcaccaagca ccaaagcttc attcttcatc tttatttgag
     2341 cggccagaaa tagggcacca gccacatca
//
LOCUS       DAOQDO010000918         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40683_length_2367_cov_2.139273, whole genome shotgun sequence.
ACCESSION   DAOQDO010000918 DAOQDO010000000
VERSION     DAOQDO010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40683_length_2367_cov_2.139273"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>874)
                     /locus_tag="PKZ53_14310"
     CDS             complement(147..>874)
                     /locus_tag="PKZ53_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41663.1"
                     /translation="AANWLLPWSAVAIGSGLRAQGHQVLVVIDSLEVWSRYVAAFPWR
                     GTLETQTGQLTDQAFATRQGSLTILALASPTLFPPVQEMFDGALDFSRALSGFPRHLN
                     SRLCRPPIKVYDMGLLGRAIAALAEIEGLSELPDSKFPAGKAQYLEQHCAWGLRMQKC
                     LQFDLTMTADSIEQLIILSLVSGFTEIPIELIPSFAKLLLARIRTECSYHLSQIRQHA
                     FYSKEDHDVFAQIASEIQSNVKP"
     assembly_gap    875..884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<885..1502)
                     /locus_tag="PKZ53_14315"
     CDS             complement(<885..1502)
                     /locus_tag="PKZ53_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41664.1"
                     /translation="MANALSSGRIIHISPTQVLADGLIAAGFGDIVEIGGNGPALVTA
                     LQDNQVELAPLRSDSMAIGTMVHSPRPLKVPVGTELCGRVLDCLGNPLDTGPYFRHTI
                     PHRVFVKHPCPILPENQPEKHHRLTTGLLVYDLKQVFLKGMALIASQDFPAVVSHLIW
                     HQVENAMIVIYARIRGGHGSETVIKEAAGRRPLPQGLARRPARGHL"
     gene            complement(1547..2248)
                     /locus_tag="PKZ53_14320"
     CDS             complement(1547..2248)
                     /locus_tag="PKZ53_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41665.1"
                     /translation="MIIEIEPLGTEILLPAIVIGYEIDFCFEHLPLGDLPDHPPWILS
                     MAQQAGGYGMSYPTCVGLVLRLLANTSHSPADLPFLIQGFQEMAEDPRIGVLKQKYLV
                     LHSMVATWGQNYTPQELTRFSAFLADYLSLPPIEGGIEAFIRFAPCHPLEYFAGWKIL
                     SVTIDTRSAPASLYQFESPQTTVYHLDSIPPQGLILADETEVSEGTMSQLLAFGKKLG
                     IETPPRVFLLWENSD"
BASE COUNT          614 a          605 c          596 g          542 t
ORIGIN      
        1 atgtggtgcg cgacgatcag gtgatcttgc tggatgccaa taaaaccatc ggtgccagcc
       61 ccaaatttgc tcacaatcca acgctgacaa aaatgtaccg ctaccgtgca gaagggattt
      121 atttttactt gaagtaattt ggagtgctac ggcttgacgt tgctttggat ttcgctggcg
      181 atttgagcaa aaacgtcgtg atcttctttc gaataaaaag catgttgcct gatttgactc
      241 agatgataag agcattcggt tcgaatccga gcaagcagta gtttggcaaa agaaggaatc
      301 agttcaatcg ggatttcagt gaatccactc accagcgaca aaataatcag ttgttcaata
      361 ctgtcagcgg tcatcgtcag atcaaactgc aaacactttt gcattctgag accccaggca
      421 caatgttgct ccagatactg ggctttgcca gcgggaaatt tgctgtccgg taactcggaa
      481 aggccctcga tttctgccag cgcggcaatg gctcgaccca ataaacccat gtcatacact
      541 ttgatcggag gccggcaaag tcttgagttc aggtgacggg gaaatccact gagtgcccgg
      601 ctgaaatcaa gggcaccatc aaacatttcc tgaactggag gaaaaagagt gggggaagcc
      661 agcgccagaa ttgtcaggct tccctggcgg gtggcaaaag cctgatcagt caattgaccg
      721 gtctgggttt ccaacgtgcc tcgccaggga aatgctgcga cgtagcgact ccacacctca
      781 agcgagtcaa tcacaaccag tacctggtgg ccctgggctc gcaaccctga accaatggcc
      841 acggcgctcc agggaagcaa ccagtttgcg gcggnnnnnn nnnntagatg cccccgcgca
      901 gggcggcgcg cgagtccttg gggaagtggc cggcgtcccg ccgcttcttt gattaccgtt
      961 tctgagccgt gaccgccccg gatccgggca taaatcacaa tcattgcatt ttcaacctga
     1021 tgccaaatca ggtgactgac tacggctggg aaatcctggc tcgcaatcag tgccattccc
     1081 ttcaaaaaca cctgcttgag atcatagacc agcaacccgg ttgtgagccg atggtgtttc
     1141 tcgggttgat tttcaggcag aatcgggcac ggatgtttta caaacacccg atgtggaatg
     1201 gtgtgacgaa agtagggtcc ggtatcaagt ggattcccaa ggcaatccag aacccgacca
     1261 cacagctctg ttccaaccgg caccttgagc ggcctgggag agtgaaccat ggttccaatc
     1321 gccatggaat ctgaccgaag cggggccagt tcaacctgat tatcctgtag tgctgtgacc
     1381 agtgccggtc cattaccgcc aatttccact atatctccaa acccagccgc tatcagacca
     1441 tcagccagga cttgagttgg agaaatatga ataatccttc cagacgaaag agcatttgcc
     1501 attcagaatc ctcgactttt ggtataactc aactctgaaa cctcggctaa tcggagtttt
     1561 cccaaagcag gaatactctg ggaggtgttt cgatgcccag cttctttcca aatgcaagca
     1621 actggctcat tgtcccttca gaaacctcgg tctcatccgc caaaatcagt ccctgaggag
     1681 gaatcgaatc aaggtgatac accgtggttt gaggcgattc aaactgatac agtgatgccg
     1741 gagcggatct ggtatctatc gtgaccgaga gaattttcca gcctgcaaaa tattcaagcg
     1801 ggtgacaggg ggcaaatcga ataaaggctt caatgcctcc ttcaatcggc ggtaagctga
     1861 ggtagtcagc caaaaaagca gaaaatcgag tcaattcctg gggcgtatag ttttgtcccc
     1921 aggttgcaac catcgaatga agaaccaggt acttttgttt caggacccca attcgtgggt
     1981 cctcagccat ctcctgaaaa ccttgaatca ggaacggcaa atcagccgga gagtgactgg
     2041 tatttgccaa aagccgaagc accaggccga cacaggtcgg atagctcata ccataaccgc
     2101 cagcctgttg cgccatgctc agaatccagg gtggatggtc aggcaggtca cctaacggaa
     2161 gatgttcaaa acaaaaatct atttcatagc caatcacgat tgcaggaagc agaatctcag
     2221 tgcccagtgg ttcaatttcg atgatcattg cgtgttcctt tcttgggaag tatagttttt
     2281 cagataacta tttcctggga acgccggcat cttgccggca ccaggcggtt cagatccaat
     2341 cagttgccgg caggatgccg gcgttcc
//
LOCUS       DAOQDO010000919         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40692_length_2366_cov_4.074859, whole genome shotgun sequence.
ACCESSION   DAOQDO010000919 DAOQDO010000000
VERSION     DAOQDO010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40692_length_2366_cov_4.074859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="PKZ53_14325"
     CDS             complement(<1..2010)
                     /locus_tag="PKZ53_14325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNJ41666.1"
                     /translation="MESTTISCPIEPVPDEPPVASSVSAETGPALHSETFPVLGMTCA
                     ACVRRIEKSVAKIPGVHQVVVNLATEQATVAFDRSLVTLTDIFSVVRDTGYDVIEPAP
                     AGADQRAEVEEAKQLETSQLRNALLLAAGLTLPLFFLEMIPMLIPPLHHVLMRVVSMQ
                     TLWYLSFVLASGVQFGPGVRFYRKGWAALRSGNPDMNSLVMIGTSAAYGYSVVATFFP
                     GSLPAGTAHVYYEASATIITLVLLGKYFEAVAKGKTSAAIKRLLALQPGIAHVVRGGK
                     EFDLPVEKVLVGDLLNVRPGEKIPVDGLVVEGKSFVDESMMTGESLPIQKEPESVVLG
                     GTVNQNGSFQMRATRVGSDTVLAQIIKTVEAAQTSRPPIQALADRVVAVFVPAVLLIA
                     ALTFLVWYIFGPAPSLSLALVNAVAVLIIACPCAMGLATPTSIMVATGKAAELGVLFR
                     KGDALQTLGEVNTILFDKTGTLTRGKPELTDLVLLPGWERSESLKLVASLEARSEHPI
                     AAAIVQHAQASDFELIPAQDVEAVPGFGIQGTIGGHRVSVGADRWMTKQGISSETLAQ
                     VAARLAGEGKTPLFAAINGKLAGVLAVADQIKPTTPEALKRLRQNGLHMVMVTGDNQQ
                     TAQTIARQLGISDIEAEILPTGKAEVVKKYQAQGHKVVFVGDGIND"
     gene            complement(2083..>2366)
                     /locus_tag="PKZ53_14330"
     CDS             complement(2083..>2366)
                     /locus_tag="PKZ53_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41667.1"
                     /translation="LRVLSLIEFTVRRALALSSQSLVGLHLENPKKPSSHPTAERLLW
                     AFRRLTLTIVSFPDRVARHLTPLTPLQIQILELLGFPPSIYSSLALDSP"
BASE COUNT          582 a          649 c          633 g          502 t
ORIGIN      
        1 atcgttgata ccgtctccga caaacactac tttgtggccc tgagcctgat attttttgac
       61 gacttcggct ttcccagtcg ggaggatttc agcttcgatg tctgaaattc ccaactgacg
      121 ggcaatcgtc tgggccgttt gctggttatc tccggttacc atcaccatat gcaacccatt
      181 ctggcgcagt cgtttcaagg cttccggcgt tgtgggtttg atttgatcgg ccaccgccag
      241 aaccccagcc agtttgccgt tgatggcagc aaagagcggt gttttccctt caccagccag
      301 ccgggcggca acctgggcca gcgtttcact tgaaattccc tgctttgtca tccagcggtc
      361 agcgccgacc gagacccgat gcccaccgat tgttccctga atcccaaacc ctggaacggc
      421 ttcgacatcc tgggctggaa tcagttcaaa gtcgctggcc tgagcatgct gaacaatcgc
      481 ggctgcaatt ggatgttctg accgggcttc aagcgaagcc accagtttta aactctcgga
      541 tcgttcccag ccgggcaaca gcaccaggtc ggttaattcg ggcttgccac gggtcaacgt
      601 tccggttttg tcaaacagga tggtgttaac ttccccaagg gtttgtagcg catcgccttt
      661 gcggaacaac acacccagtt cggcagcctt cccagtggca accataatcg aagtcggcgt
      721 tgccagcccc atggcacagg gacaggcaat aatcagaaca gccacggcat tgaccagcgc
      781 caatgaaagc gacggtgcgg ggccgaagat gtaccagacc agaaacgtca gcgcggcaat
      841 cagaagcacc gccgggacaa acactgccac cactcgatct gccagtgcct gaattggagg
      901 ccgtgacgtt tgcgcggctt ctacggtttt gatgatctgc gccagaaccg tatctgaccc
      961 aactcgtgtt gcccgcattt gaaagcttcc attctggttg accgtaccgc ccaaaacgac
     1021 gctttccggc tctttttgga tcggaagtga ttcgccggtc atcatggatt catccacaaa
     1081 cgacttcccc tcaaccacca ggccatccac cgggattttc tcgcctggac gaacattgag
     1141 cagatctccg accagtactt tttcaaccgg aagatcaaat tctttcccgc cacgcaccac
     1201 gtgggcgatg cctggttgta aagcaagcag gcgtttgatc gccgcactgg ttttgccctt
     1261 ggcgaccgct tcaaaatact ttccaagtaa gaccaacgtg atgattgtgg ctgaagcctc
     1321 ataataaacg tgtgctgttc ctgccggcag tgagccggga aaaaacgttg ccaccactga
     1381 atacccgtag gcggcgctgg tgccgatcat caccagcgag ttcatatccg ggttgcctga
     1441 ccgaagtgcc gcccagccct tccggtaaaa tcgaactccc ggcccgaact gaacgccgct
     1501 ggccagcaca aatgacagat accacagcgt ttgcattgaa acgacgcgca tcaagacgtg
     1561 atggagtggc ggaatgagca tcggaatcat ctcaaggaaa aacaagggca gcgtcaaccc
     1621 ggcagccagc agaagtgcat tccgcaactg gctggtttca agttgttttg cttcttcaac
     1681 ttcggctcgc tgatcagctc cggctggggc aggttcgatc acgtcataac cggtatcacg
     1741 cacgacggaa aaaatgtcgg tcagcgtcac cagactccgg tcaaaagcca cggtggcctg
     1801 ttcggtggcc agattcacca ctacctgatg cactccgggg attttcgcca ctgatttttc
     1861 aatccggcga acacaggcgg cacaggtcat gccgagcaca ggaaaagtct cgctgtgcaa
     1921 agcgggtcca gtctcggctg aaactgaaga ggcaaccgga ggctcatcag gcacgggttc
     1981 aattgggcaa gaaatcgttg tggattccat agactcacca ttcgcgtaat aaaaatctag
     2041 tttctcgaat tctttttcca gttaagggca aaagcacgca agttaaggag aatccagagc
     2101 cagggaagaa taaatcgagg gaggaaaccc gaggagttct aaaatctgga tttgaagagg
     2161 agtcagaggg gtcaggtgac gggcaacgcg gtcgggaaaa gaaacaatgg tcaaagtgag
     2221 gcgacgaaaa gcccataaga gacgctcggc agttgggtga gaggaaggtt ttttgggatt
     2281 ttcgagatga agaccaacca gggattgaga cgaaagggcc aaagcgcgac gaaccgtgaa
     2341 ttcaatcaag gagagaacgc gcaagg
//
LOCUS       DAOQDO010000920         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40727_length_2365_cov_3.142424, whole genome shotgun sequence.
ACCESSION   DAOQDO010000920 DAOQDO010000000
VERSION     DAOQDO010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40727_length_2365_cov_3.142424"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="PKZ53_14335"
     CDS             complement(<1..1767)
                     /locus_tag="PKZ53_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41668.1"
                     /translation="MKERLPQLPIFSFPGINHLRGLVYVLLLTGFIGTIDSLPLQAQS
                     AHPSEIAAEYTRRAATLRQKWHLNEAERAFETARVADKNNISALLGLAEIARIKLDYQ
                     QSLEFLHQAAFLAQARLEPESVASKAVDETYLSLLNSYGILYLTIEDPVTAGQFFEKI
                     LQISPQDSRAWLGKATVALLNRDYAQSENILTRLSTKRPNDSSVYSTLAKVFLEQNRY
                     PQASSAAQRALDLDPFNLEAKSVLCSVRVIERNPEAVRKLAQEVLDLNPYNARIRRLF
                     SQYIRSRKAMPAVLPEAQKYFEKASDFARRKEIERAIEAYEQATQLSPAFLQAYLGLG
                     ACALSDGNLELATVAAEQATTIDPENALAQLQLSLAHAELHERARKAVGINLPDLPAP
                     PEQTAPTDIGEVFVNYFGLAADQQRIINLSVAPFAHLLPELKRRGAKHYLLPLDRQLP
                     EIAGYEMLEDRTTFDGRYYSSIRGVGGLVTVSGIEYLNVVEQGGFNTIAHEFAHQVHS
                     SVFDKPLLERIQALYAKAVREGRTLDYYAAANEWEYFAQGYEAYVSISKRPGLGITGR
                     HTRDELRKLDPALYTLIEELANP"
     gene            complement(2158..>2365)
                     /locus_tag="PKZ53_14340"
     CDS             complement(2158..>2365)
                     /locus_tag="PKZ53_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HNJ41669.1"
                     /translation="DPAFVAILGLTAGSCGTLLSPMAANFNLLPAYLYEMKNPYGIIR
                     HQWKFALGLWACHAVILATWLFFR"
BASE COUNT          605 a          509 c          650 g          601 t
ORIGIN      
        1 ggggttggcc agttcttcaa ttaaagtata cagagccgga tcaagtttcc ggagttcgtc
       61 acgggtatga cggccagtga ttcctaaccc agggcgcttg gagatggaca cataggcttc
      121 atacccctgg gcaaagtatt cccactcatt ggctgctgca tagtaatcaa gggtacgacc
      181 ttctcgaact gctttggcat acagtgcctg gatgcgttcc agaaggggtt tatcaaacac
      241 actgctatga acctgatggg caaattcatg ggcaatggta ttaaacccac cctgttcgac
      301 cacattcagg tattcaattc cagaaactgt gaccagtccc ccaaccccgc gaatactgct
      361 gtaatatcgg ccatcaaacg tggtccgatc ttcaagcatt tcataccccg cgatttcagg
      421 aagctggcga tcaagtggga gcagatagtg tttggccccg cgtcgcttta attcaggaag
      481 caaatgggca aacggggcga ccgaaaggtt gataattcgc tgctggtcag cggcaaggcc
      541 aaaataattg acaaaaacct cgccaatatc agtgggggcc gtttgttcag gcggagctgg
      601 aagatctggc aggttgattc caacagcttt gcgggcacgt tcgtgaagtt cagcgtgtgc
      661 aaggcttaac tggagttgag ctaaagcgtt ttctggatca attgtggttg cctgttcggc
      721 tgctacggtc gcgagttcaa ggtttccgtc tgaaagtgca caggctccaa gcccaaggta
      781 ggcctgaaga aaagccggtg aaagctgggt tgcttgctca taagcctcaa ttgctcgttc
      841 tatttcctta cgccgggcaa agtcagaggc cttctcgaaa tatttttggg cttcgggtaa
      901 gaccgccggc atagcttttc gactacggat gtattgtgaa aaaagccttc gaatccgggc
      961 gttatatgga ttaaggtcaa gtacttcttg agctaacttg cgaactgcct ctgggttgcg
     1021 ttcaatcacc cggactgagc acagcacaga ttttgcttcg aggttaaagg gatccaggtc
     1081 aagtgcgcgt tgggcggctg aagatgcctg agggtaacgg ttttgttcaa gaaaaacctt
     1141 ggctaacgtt gagtaaaccg atgaatcatt tggtcttttg gtcgaaagtc tggtgaggat
     1201 attctcactt tgagcataat cacgattgag gagtgccacg gtggctttcc ccagccatgc
     1261 ccgtgagtcc tgtggtgaga tctgaagtat tttctcaaag aattgcccgg cagtgactgg
     1321 atcttcaatt gttagataga gtatgccgta cgaattgagt aatgacaggt aggtttcatc
     1381 aacagctttt gaggcaactg attcaggttc caggcgtgcc tgggccagga aggcggcctg
     1441 gtgcagaaat tcaagtgatt gctggtaatc gagtttgatt cgggcgattt cggccagccc
     1501 taacagggct gagatattat ttttatctgc aacccgggct gtctcaaacg ctcgttcagc
     1561 ttcgtttaaa tgccattttt gccgcagtgt cgctgcccgc cgggtatatt cagccgcgat
     1621 ttcgcttggg tgggctgatt gtgcctgaag tggaagtgaa tcaatggtgc cgataaagcc
     1681 agtcagcaag agaacataca caaggccacg aaggtggttg atgccaggaa aagagaagat
     1741 gggaagttga ggtaagcgct ctttcatgac gatatagatc gtggactcct gtaggtaagc
     1801 taggccgaaa gaagaaattt gaagggatgt cgcctggatc aagaaccagt tgatggagag
     1861 gtattgcggt gtccagttgc cgtactggag tggttgagtc ctgaggacag catcaccttc
     1921 acaagacaaa ttgtttggat tgaatgggct gaaacatcag aaacacaagg aattgaatta
     1981 ggaaggtgtt tctgtttggt agagattttg gatttctccg accactaaaa taatactgaa
     2041 tgtgaaatca atcaatcaat tttttgaatg actattcagg atttcggaaa ttttagcttt
     2101 cctggggggc agagggaggt ttttgctgaa aagagagcac cagatcccgg ttttttttca
     2161 tcgaaagaag agccaggtgg ccaaaatcac ggcatggcag gcccacaacc ccagagcaaa
     2221 tttccattga tggcgaatga tcccataggg gtttttcatt tcataaaggt aggccgggag
     2281 cagattgaag ttcgccgcca ttggggaaag cagggtgcca cagctcccag ccgtcaaacc
     2341 caaaatggcg acaaacgccg ggtca
//
LOCUS       DAOQDO010000921         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40797_length_2363_cov_3.486568, whole genome shotgun sequence.
ACCESSION   DAOQDO010000921 DAOQDO010000000
VERSION     DAOQDO010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40797_length_2363_cov_3.486568"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..360)
                     /locus_tag="PKZ53_14345"
     CDS             complement(58..360)
                     /locus_tag="PKZ53_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HNJ41670.1"
                     /translation="MPPAARLTDFHQCPLSTPVGPVTIPHVGGPIAGPGAPTVLIGGL
                     PAAKVGDIAICIGPPDAIVKGSMTVKVMGMPAARMGDKTAHGGTILMGFPMVMIGG"
     gene            complement(485..2287)
                     /gene="vgrG"
                     /locus_tag="PKZ53_14350"
     CDS             complement(485..2287)
                     /gene="vgrG"
                     /locus_tag="PKZ53_14350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HNJ41671.1"
                     /translation="MTTPTPYSVDTSLVSFSIKIEGKEIDTTFQVYSINTWVGVNKIP
                     KAQILIYDGSPSEQTFEISNLSTFLPGKKLEIAAGYNNGKLTPIFKGVIVKQGIEITQ
                     TQGSKLIVDVTDEAIKMTLDRKNALFEKIKDSDLIGKLITSNGLAKDVAATTTVYEEI
                     VQYYATDWDLMLMRAELNSMVAIVDGGKVTVKKPDTQQSPVLKVEYGDTILDLQAEMN
                     AATQYKSSVIKSFTWDVDTQKLIESGPGSVSVQEPGNVSSDELARVFDVKKYTQQTGG
                     PVEKSSLQDWSSAEVLKSKLSKIRGHVRFQGSALVQPGKTIELAGLGNRFNGKAYISG
                     VHHSIINNKWLTSVDFGLSARWFAAENPDISAPDASGQLPPIKGLQTGIVKKVAQDPG
                     GEFRVLVNLPILQDASKGVWARLATFYASNKVGAVFYPEVNDEVVVAFMNEDPRYPVI
                     VGSLYSKKMAPPYAPDEKNQKKAIVTKSKLEITFDEQNKIIEIKTPGKHLIKLDDKAG
                     SITIADSNRNSVVLSKSGINLESASNINIKAKGNISLDATGNLSLSAKANATMEGLQV
                     AHKAKAKFSANGTASAEITSSGILTVRGTLVKIN"
BASE COUNT          528 a          587 c          599 g          649 t
ORIGIN      
        1 ggaacccgga acccggaacc cggaacccgg aacccggaac ccggaacccg gattttctca
       61 tcctccaatc atcaccatcg gaaagcccat caaaatagtg ccgccgtggg cagttttgtc
      121 gcccattctg gccgcgggca ttcccatgac tttgaccgtc atcgagcctt tcacaatggc
      181 atctggagga ccaatgcaaa ttgcgatgtc acccactttg gcggcgggca atccaccgat
      241 taagacggtt ggcgcaccgg ggccggcaat tgggcctccg acgtgcggaa tggttacggg
      301 gccgaccggg gtgctgagcg gacattgatg aaaatcggtc aatctggcag cgggaggcat
      361 aggaaaactc ctttattgac cagggattgg tacctggtac ctggtacctg gtactttgga
      421 accaatattt tcaaaaacca aaaaccaagc accaagcacc aggcaccaaa cacctgtttc
      481 cccttcaatt gattttaacc agcgttccac ggacggtcag aattcccgaa gaggtaattt
      541 ctgccgaagc ggtgccgttg gctgaaaatt tggctttagc tttgtgggcg acctgcaacc
      601 cttccattgt cgcgttggct ttggcgctta atgatagatt gccagtcgca tctaagctga
      661 tatttccttt ggctttaata tttatgtttg aagcgctttc aagattgatt ccgctttttg
      721 aaagcacaac ggagtttcgg ttactgtcgg caatggtgat ggacccggct ttgtcatcca
      781 gtttgatcaa atgttttccg ggggttttga tttcgatgat tttattttgt tcgtcaaagg
      841 tgatttcgag cttgcttttg gtcacaatgg ctttcttctg gttcttttca tctggcgcgt
      901 agggcggggc catctttttg ctgtatagac tgccaacaat caccgggtag cgcgggtctt
      961 cattcataaa agccacgaca acttcatcat tgacttcggg gtaaaacacc gctccaactt
     1021 tgtttgaagc ataaaaggtg gccagtcgcg cccaaacgcc tttgctcgca tcctgtaaaa
     1081 tgggcagatt gaccagcact cgaaattctc cgcctggatc ctgggctact tttttgacaa
     1141 tcccggtctg caagcccttg atcggaggta gttgcccgga ggcatccggg gcggaaatat
     1201 cgggattttc cgccgcaaac cagcgggcag acagcccgaa gtccactgat gtcagccatt
     1261 tattgttgat aatcgaatga tggacgccgc tgatataggc ttttccattg aatcggtttc
     1321 ctaatccggc gagttcgatg gtcttgcccg gctggaccag cgcgctgcct tgaaatcgga
     1381 cgtgcccccg aattttagac agttttgact tgagtacttc ggcggatgac cagtcctgga
     1441 gcgacgattt ttccaccggg ccgccggttt gctgagtgta tttcttgacg tcaaagactc
     1501 tggccagttc atcggatgaa acgtttcccg gttcctgaac cgagaccgag ccaggtccgg
     1561 attcgatcag cttttgggtg tctacgtccc aggtaaagct tttaatgact gaagatttgt
     1621 actgggtggc cgcgttcatt tcagcctgca agtccagaat tgtgtcgcca tattcgactt
     1681 tcaacacggg ggattgctgg gtatcaggtt ttttgaccgt aactttgcca ccgtccacaa
     1741 ttgcgaccat gctgttgagt tcagcgcgca tcagcatcag gtcccagtcg gtggcatagt
     1801 actggacaat ttcttcataa acagtggtgg ttgcggcaac gtctttagct aacccattgc
     1861 tggtaatgag tttcccaatc aagtcgctgt ccttgatctt ttcaaagagg gcatttttgc
     1921 ggtcaagagt cattttgatg gcttcatcgg ttacatccac gatgagcttt gaaccctggg
     1981 tttgggtgat ttcaatgccc tgtttgacaa tcacgccctt aaaaatcgga gtgagtttcc
     2041 cgttattgta cccggctgca atttcaagtt ttttcccagg cagaaacgtg gacaggttgc
     2101 tgatttcaaa ggtctgttct gacggactgc cgtcataaat cagaatctga gctttgggaa
     2161 ttttgttgac gccaacccag gtgttgattg aatacacctg aaaggtggtg tcaatttcct
     2221 tcccttcaat cttaatgctg aaggaaacca gtgacgtatc aactgaatag ggggtcggcg
     2281 tggtcattgg accgtggctc cttcaaacgg tggaaataca atctgcatcc cgatcaccag
     2341 gctccggaaa tcagtcaatc cat
//
LOCUS       DAOQDO010000922         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40845_length_2361_cov_4.017346, whole genome shotgun sequence.
ACCESSION   DAOQDO010000922 DAOQDO010000000
VERSION     DAOQDO010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40845_length_2361_cov_4.017346"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(509..2041)
                     /gene="accC"
                     /locus_tag="PKZ53_14355"
     CDS             complement(509..2041)
                     /gene="accC"
                     /locus_tag="PKZ53_14355"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNJ41672.1"
                     /translation="MFQKILIANRGEIAVRIIRACRDLGIASVAVYSDVDQNALHVRL
                     ADEACHIGPSPAMQSYLVTERILEAAHKTGAQAIHPGYGFMSENAEFARAVEAAGLVF
                     IGPPPSAIDAMGNKISAREIAIRAGAPVVPGVQDPVDTIEAALVVAEQIGFPIMLKAA
                     AGGGGKGMRIIRTPAELPSAFDLARSEAVSSFKDGTIYLERYIERPRHIEIQLMGDQH
                     GNLVYFGERECSIQRRNQKVIEECPSPLNDPELRRLMGESAVRVAKTVGYFSAGTTEF
                     LVDPNRNFYFLEMNTRLQVEHPVTELVTGIDLAALQIRVAAGEKLPFRQEDIHLRGNA
                     IECRVYAEDPDRNFMPAPGLISAWVPPEGPGVRVDSGVYEGWNIPIDYDPLLAKLICW
                     GETREQAISRLDRALREFVLCGVRNNLDFFRQIAEDPEFRAGTLDTGFIGRFFERLNQ
                     AKTTTSEDPLPTEADLVRILAGLLHHSQAATHSNPTGNNGLSQESAWKRRGWLQAQAS
                     RF"
BASE COUNT          538 a          644 c          618 g          561 t
ORIGIN      
        1 ctcccattcc tgtttaacct gtcaccgtag tgcggcggac tgaggcggca tccgctggta
       61 cctatatatt cttcccaatc gtttcccgtg atttgcacgg ggatctggtc aactggagct
      121 tgaacccatc tccccacacc ggccatccgt caggatggcg tcgtggcgac acgactcaag
      181 ctcggccctt cagggccgag tctgttgaca ggttttcttc agtttttcaa ttgaaatgat
      241 accagtcagt agtcagtagt cagtagtcag tagttcacta agcttatttg gttgaatcac
      301 ttgactgttt ttttatccga ggattccatt gcaaattggt atgattgagc cagggaaatg
      361 aagaatgaag catgacaatc caacttcttc aacccgatgt cttcagctcc aagccctggt
      421 tttttcagcc caacttcttc agcccccagc ccccagcccg aagtcttcag cccccagccc
      481 tgagccctgg tttcctgagc tgactgcttc aaaaccggct ggcctgggct tgaagccagc
      541 cccggcgttt ccaggcgctt tcctgtgaca agccgttatt tcccgttggg tttgaatggg
      601 tggcagcctg actatggtgt aacaggcccg caaggattct gaccagatcg gcttcagtcg
      661 ggagaggatc ttcagaggtt gtcgttttgg cctggttgag ccgctcaaaa aaccgaccga
      721 taaacccggt atccagggtt ccagcgcgaa actcggggtc ttcggcaatc tggcggaaaa
      781 aatccaggtt attgcgcaca ccacagagga caaactcacg caatgcccga tccaggcggg
      841 agatcgcctg ctctcgtgtt tctccccagc agataagttt ggccaggagc gggtcataat
      901 caatcggaat attccatccc tcatacacgc ctgaatcaac tcgaacccca ggcccctcgg
      961 ggggaaccca ggccgagatc agacctggtg ctggcataaa attgcggtct ggatcttcgg
     1021 catacacccg gcattcgatg gcattgccac gcaagtgaat atcttcctgg cgaaatggca
     1081 gtttttcgcc cgcggccact cgaatttgaa gcgctgccag atcaatgccc gtgaccagtt
     1141 ccgtcactgg atgctcaacc tgaagccggg tattcatctc caggaaatag aaattgcgat
     1201 tgggatccac cagaaattcg gtggttccgg cactaaagta gccaactgtt ttcgcaactc
     1261 tgacggctga ttcgcccatt aaccgccgga gttccggatc gttgagtggg gaagggcatt
     1321 cttcgatgac tttctggttg cggcgttgaa tcgagcattc acgttcacca aagtaaacca
     1381 ggttgccgtg ctggtcaccc atcaactgga tttcaatatg gcgtgggcgt tcgatatagc
     1441 gttccagata gattgtgcca tctttaaatg aggaaactgc ttctgatcga gccagatcaa
     1501 aggccgaggg cagttccgcc ggggttcgga taatccgcat gcctttgccg cccccgccgg
     1561 cagcggcttt taacataatc ggaaacccga tttgttcggc cacaaccaga gctgcctcaa
     1621 tcgtatcaac cggatcctgg accccgggaa ccaccggggc gccggcccga attgcaattt
     1681 cgcgggcact gattttattg cccatagcgt caatggctga cggtggcggg ccgataaaca
     1741 ccaggccggc agcttcaacc gcccgggcaa actcggcatt ttccgacata aatccatatc
     1801 ccggatgaat cgcctgagcc ccggttttat gggcggcttc gaggatgcgc tccgtaacca
     1861 gataactttg catcgcgggt gacggcccaa tgtgacaggc ttcatctgcc agcctgacgt
     1921 gcagggcatt ctgatcgaca tcggaataaa cggccaccga ggcaattccc agatctcgac
     1981 aggcacggat aatacggacc gcaatttcac cacggttggc aatgagaatt ttttgaaaca
     2041 taagcttggt ggaaggtgtc tcgttgggaa atgagtgctt tagggcgtcg gaaactcagg
     2101 tatcttttct ggccgatggt gaatcaaggc acaccagaca cccgatcaat ctcgatcaag
     2161 cgttgagatt tctgatctaa agcaaagaga aaggtggggc aaccagggat tttaagatta
     2221 gacaacgatt gcgtttccgg gtcttgcagg gtggtatcgc acgctcacgc ggcttcccac
     2281 gacataggcc cgaaggtttg ccatttggtt ggtatcaagc gtctgacagg attcataggt
     2341 gacgcctgat acctgatacc g
//
LOCUS       DAOQDO010000923         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40866_length_2361_cov_2.166088, whole genome shotgun sequence.
ACCESSION   DAOQDO010000923 DAOQDO010000000
VERSION     DAOQDO010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40866_length_2361_cov_2.166088"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PKZ53_14360"
     CDS             complement(<1..1129)
                     /locus_tag="PKZ53_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41673.1"
                     /translation="MRDILPIETDILIVEDSLTQAAQLQFILEKQGFQVRIAPDGREA
                     LNEIERQSPTLVISDIVMPVMNGYELCRFIKQKLEWRHIPVILVTNLSNPEDIIKALE
                     CEADNLIRKPYDPGYLISRINNLLSTRQIRQNDRIQMGLKFRFGGSTYVIDTDRTQIL
                     DLLLSTFENAILQTRDLMNAQNDLRLANQKLEEKAQALETSERNYKVLLESNSDGMLV
                     VDTGGRVLFVNQAAERLTGIESKRLLGTKFGFKLTSGVRQEMEFGAAQAGAPSLTVEM
                     NTVDTLWEGQPAFLVTLHDMTIRKLAQTQLQQAKELAESANKAKSEFLATMSHEIRTP
                     MNGVIGMAELLRNTPLTSEQSEYVEAIQHSGQALLGIINDIL"
     gene            complement(1122..2174)
                     /locus_tag="PKZ53_14365"
     CDS             complement(1122..2174)
                     /locus_tag="PKZ53_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNJ41674.1"
                     /translation="MINVLIADDSPATQMYLQFLLEQDGELSVVGLANDGRQAISLTS
                     RLKPDVIVMDIHMPGVNGFEATRILMQSTPLPIVIVTSAYDPRDSVVNFQALEAGALT
                     LVGKPTGLGHPKQKETSQELIATVKAAARARLNSGPRLAPGVRPAVQSTTLALPRREP
                     LKILAIGASTGGPPVIRDLIQRLIFPFPAPILIVQHITAGFIEGFRDWLAQTTNWPVH
                     MATHHLRAHPGTIYLAPDDHHLTITPGFDLVLKQDVAEHSLRPAVSVLFRSIAQSFGS
                     GSAGVLLTGMGKDGAQELRLIRDRGGITFAQDQATSVVFGMPGEAVKLKAATHVLPPE
                     EIAGILNLLVKGSFHA"
BASE COUNT          556 a          590 c          620 g          595 t
ORIGIN      
        1 cgagaatgtc attgatgatt ccgagcaagg cttgtccaga atgctggatg gcttcgacat
       61 attcggattg ttccgaggtc aatggtgtgt tgcgcaataa ctcggccatg ccaatgacac
      121 cgttcatggg agtgcgaatt tcatggctca tggtggccag aaactcactc ttggctttat
      181 tggccgattc ggccagttct ttggcttgct ggagctgggt ctgagccagt ttacggatgg
      241 tcatatcgtg cagcgtgacc aaaaaagccg gttggccttc ccagagggta tcaaccgtgt
      301 tcatttcaac cgtcagtgag ggggcaccag cctgggcagc gccaaattcc atttcctgcc
      361 ggaccccaga ggtcagctta aatccaaatt tcgtccccag caggcgctta ctttcaatcc
      421 cggtcagtcg ttcggcggcc tggttgacaa acagaacccg gccaccggtg tcaaccacca
      481 gcatcccgtc gctgttactt tcgagcagca ccttgtaatt gcgttcggac gtctcaaggg
      541 cctgggcttt ttcctccagt ttctggtttg ccagccgcag gtcattctgg gcattcatca
      601 aatcacgggt ttgcaggatg gcattttcaa aagtagataa aagcaagtcc agaatttgcg
      661 ttcgatctgt gtcaatgaca taggttgagc cgccaaatcg aaatttcaac cccatctgaa
      721 tgcggtcgtt ttggcgaatt tgacgggttg aaagcaaatt gttgatccgc gaaatcaaat
      781 atcctggatc atagggcttg cgaatcagat tatcggcttc gcattccagg gctttgatga
      841 tgtcttccgg gtttgagaga ttggtaacca gaatgaccgg gatgtgtcgc cactccaatt
      901 tctgcttaat gaaccgacac aattcatagc cattcatgac tggcatcaca atatcgctga
      961 tgaccagggt cggtgactgg cgttcgattt cgtttaatgc ctcgcggcca tcaggggcga
     1021 ttcgaacctg aaacccttgc ttttcaagga taaactggag ttgtgctgct tgagtcagac
     1081 tatcttcaac aatcagaata tctgtctcaa tcggcagaat gtcacgcatg gaacgaccct
     1141 ttcacaagca aattgagaat cccagcgatc tcttcggggg gaagaacatg agttgcagcc
     1201 ttgagtttga cggcttcgcc tggcattcca aaaaccacgg atgtggcctg atcttgtgca
     1261 aatgtaatgc caccgcgatc tcggatcagc cttaattcct gagccccatc ttttcccatg
     1321 ccagtgagca ggactccggc gctgcctgac ccaaaagact gggcgataga tcgaaataaa
     1381 actgataccg ctgggcgcaa actatgctca gctacatcct gtttgagcac taaatcaaat
     1441 ccaggtgtaa tggtcaaatg atgatcgtct ggggcgaggt aaatcgtgcc tgggtgagca
     1501 cgtaaatgat gggttgccat atgcacgggc cagttggttg tctgagcgag ccagtcgcga
     1561 aatccctcga taaatccggc agtaatatgt tggacgatta aaatgggggc tggaaatgga
     1621 aaaatcagac gctggatcag gtcccgaatg acgggtgggc caccggttga ggcaccaatg
     1681 gccaggattt tgagtggttc acgcctcggc aaagccaacg ttgtggattg aactgcggga
     1741 cggacgccag gtgccagccg tggcccggag ttgagcctgg cgcgggccgc cgccttcacc
     1801 gtggcaatga gttcctgaga cgtttctttt tgtttgggat gtcccagacc cgtgggtttc
     1861 cccaccagcg tcaacgcacc ggcttcgagt gcctgaaaat tgacgacact atccctcgga
     1921 tcataggcgc tggtgacaat gacaatcgga agtggcgttg attgcatcag gatccgtgtt
     1981 gcttcaaatc cattgacccc aggcatatga atatccatca cgatcacatc cggcttgagc
     2041 cggctggtca gcgaaatggc ctgtcgcccg tcattggcca gccccacaac actgagttca
     2101 ccatcctgct caagcaaaaa ctggaggtac atttgggtgg ctggtgaatc atccgcaatt
     2161 aaaacgttga tcataatttg ttgaggaaag gagcggagga gggacaaagt aagggggtga
     2221 ataggaaagc atcaggttct agctctgatt tttcaacgtc ttaaactctt ttggtttcgc
     2281 tatttcgcta tttcgcttat ttcactattc actctttcgc tcacttgcct gttccgctca
     2341 gacggctaca gcaaccgccg a
//
LOCUS       DAOQDO010000924         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40916_length_2359_cov_3.785156, whole genome shotgun sequence.
ACCESSION   DAOQDO010000924 DAOQDO010000000
VERSION     DAOQDO010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40916_length_2359_cov_3.785156"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /locus_tag="PKZ53_14370"
     CDS             <1..1951
                     /locus_tag="PKZ53_14370"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNJ41675.1"
                     /translation="TYDFKDLKDVLQSTYGVPIFQEQIMAIFQKLAGYSLGEADLVRR
                     AMGKKKREELDAHKAKFFQQAEDRGHDRGKLEKLWQSLEGFADYAFNKCLAGDAPIVD
                     ADTGQIVTISEIAEGTVSTSRTFSFDGSQIITNEIIEAFPTGEKEVVELELENGRTLR
                     CTLDHKFFTDQGYLPLKDIIEQNLEIYFSEEVEAFTASSLEAPGQYPQNRVTIRLKKM
                     KLVRVNPSGTEKTYNMTMREPHHNYFTNGILTANSHSACYGVLAYQTAYLKAYYPAHF
                     WAAVLSNELNNTDKVAKYIERARAQGIEILPPDVNLSYHGFTSTEKTIRFGLMAIKGI
                     GEAAVDAIVEARKDGPFTSIYDLAKRVDSRALNRRVLESLIKSGGLDEHPGTRAQKFA
                     AIESVMESGARAQRDAQSGQVSLFGMLEAEMEADADATLPDVPPWSQQEQLAGEKATL
                     GFYITGHPLASYRELLGTFSNVSYETLNKCQPNQTIKMGGMVVGYVVKNTKKGDRFCV
                     FSLEDELGSIEVIAWPETFKRISGKISDTQAVLVTGRIEFTDDGPSKIIADDVEPLAG
                     LRERSASLLTFYFRSGSITAEKVDQLRSLFDRHRGDCQISFEVQLPTGDIACVRPNQF
                     VRVKSSPELVQAVQNLCAGCEVRMS"
     gene            2063..>2359
                     /locus_tag="PKZ53_14375"
     CDS             2063..>2359
                     /locus_tag="PKZ53_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41676.1"
                     /translation="MTRPHSPTLYRQLTRFFFHTCPVLILAVMCVNGISGSLAQAMAH
                     QNTPSAVPKVRPTDVESVDAIIKTLYEVISGPAGQKRDWDRFRSLLHPSARLIPT"
BASE COUNT          622 a          593 c          585 g          559 t
ORIGIN      
        1 aacatatgac tttaaggacc taaaagacgt attacaaagc acttacggag ttccgatttt
       61 ccaggaacaa atcatggcga ttttccagaa actggctgga tattcgctcg gagaagctga
      121 cctggttcga cgcgccatgg ggaagaaaaa gcgtgaggaa ctcgatgcac acaaggccaa
      181 attttttcaa caggctgaag atcgcggtca cgaccgggga aaactcgaaa agttgtggca
      241 gtcgctcgaa ggatttgcgg actatgcttt caataaatgt ctcgctggtg atgctcccat
      301 agttgatgct gacacaggtc aaattgtaac catttctgaa attgccgaag gaactgtttc
      361 aacctcacgc acattcagtt ttgatgggag ccaaatcatc accaacgaga tcattgaggc
      421 gtttccaact ggtgaaaaag aagttgttga gctagagctt gaaaacggac gaacccttcg
      481 ctgtactctt gaccataaat tctttactga ccagggttat ttgccattaa aggacatcat
      541 tgagcagaat ctggaaattt atttttcaga ggaggttgaa gccttcaccg ccagcagtct
      601 ggaggcacct ggtcaatacc ctcaaaatcg agtcactatt cgcttgaaga agatgaaact
      661 ggtgcgggta aatccgtcgg ggacggaaaa aacctacaac atgaccatgc gtgagccaca
      721 ccacaactac ttcacgaatg ggattctgac agccaacagt cactccgcct gctatggggt
      781 ccttgcctat cagacagctt atcttaaagc atactatcca gcgcacttct gggctgctgt
      841 tctgtcaaat gaattaaata acactgacaa ggtggccaag tatatcgaac gcgcccgcgc
      901 tcaaggaatt gaaatcctgc caccggatgt caacctcagt tatcacgggt ttacttcaac
      961 tgagaaaacc atccgttttg gactgatggc gattaaaggc attggcgaag cagccgtgga
     1021 tgctattgtc gaagcccgca aagatggccc atttacgtca atttatgatc tggccaaacg
     1081 ggttgacagc cgggcactca accgacgggt gctggaaagc ctgatcaaat cgggagggct
     1141 cgacgaacat cctggtactc gcgctcagaa atttgcggcg attgaaagcg tgatggaaag
     1201 cggcgcccgg gcgcaacgcg atgcccagag cgggcaggtc tcgctttttg gaatgctcga
     1261 agccgagatg gaagccgatg ctgatgccac actgccggat gttcccccct ggtcacaaca
     1321 agagcaactg gccggagaaa aagccacact gggcttctat atcaccggcc atccactggc
     1381 cagctatcgc gaattgctcg ggacgttttc caatgtcagc tatgaaacac tcaataaatg
     1441 ccagcccaac cagaccatca aaatgggcgg gatggtggtc ggctatgtgg tgaaaaatac
     1501 caagaaaggc gaccggtttt gcgtcttctc gctcgaagat gaactgggct caatcgaagt
     1561 gattgcctgg ccggaaacct ttaaacgcat cagcggcaaa attagtgata cccaagcagt
     1621 gcttgtaact ggtaggattg aatttaccga tgatggtcct tcgaaaatca ttgccgatga
     1681 cgttgaacca ctcgccggcc ttcgggaacg atccgcttcg ctgctgacct tctacttccg
     1741 gtcaggcagt atcaccgccg aaaaagttga ccaacttcgc agcctgtttg atcgccaccg
     1801 gggtgattgc cagattagct ttgaggttca gttaccaacc ggagatattg cctgtgtgcg
     1861 cccaaaccag tttgtgcgtg tcaaatcaag cccggaactg gttcaggcag ttcagaacct
     1921 gtgtgctggc tgtgaagttc ggatgagtta aacgatcagt ggccgggaat gaagtgtgaa
     1981 gcaagctttt tgttgaccca caacgacctg gttcattctt cattcttcat tcttcattcc
     2041 tattctaagg atcatcctga ccatgaccag gccacattca cccacgcttt accgccagtt
     2101 gaccaggttt ttctttcaca cgtgcccggt gttgatcctg gcggtgatgt gtgtaaatgg
     2161 aatcagcggc tctttggctc aagctatggc acaccaaaac actccctctg cagttcccaa
     2221 ggtacggcca actgatgttg aatcagttga tgccatcatc aaaacgctct atgaagttat
     2281 ttcaggcccc gccggtcaaa aacgtgactg ggaccgattc cggtcgctgc tgcacccgtc
     2341 ggccagattg attccaacc
//
LOCUS       DAOQDO010000925         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40932_length_2359_cov_2.672743, whole genome shotgun sequence.
ACCESSION   DAOQDO010000925 DAOQDO010000000
VERSION     DAOQDO010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40932_length_2359_cov_2.672743"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKZ53_14380"
     CDS             complement(<1..187)
                     /locus_tag="PKZ53_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41677.1"
                     /translation="MPWKVVERRIGRAGGIKQRTARQREWNHKYGDGMWQIGYVIDGE
                     FVTQEDALETIYYRSYEA"
     gene            complement(444..959)
                     /locus_tag="PKZ53_14385"
     CDS             complement(444..959)
                     /locus_tag="PKZ53_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41678.1"
                     /translation="MEQQPYSYQQPVSANYAGYQPDPVSVTLIDPVKQELLLKRIHNG
                     GNWFFWVAGLSLINSVIQHLGGDVSFVLGLAATQIIDAGFRMFTGPLGMVVSIFLDVM
                     VAGLFVFFGVLARHRSYWGFVPGVALYALDGLLYTFLGSALAVVFHIYVLICIGAGFL
                     AERELRACERT"
     gene            complement(986..1498)
                     /locus_tag="PKZ53_14390"
     CDS             complement(986..1498)
                     /locus_tag="PKZ53_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41679.1"
                     /translation="MRFIRRMVWGWCLVLVLSGSTALLAEKPTSPEKKAKMIALINRM
                     ESNPYHSDSKKMASEVLSWLADAPDVTVTVCTDVLATEKNDLDSDESTFLFGHFIFSQ
                     ARFIVEHPDQASDLVAVNRAGVDGILKAYATMKVIKPKLKVKFADQLLSLRETGGLDR
                     FLQQALAKCN"
     gene            complement(1532..>2359)
                     /locus_tag="PKZ53_14395"
     CDS             complement(1532..>2359)
                     /locus_tag="PKZ53_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41680.1"
                     /translation="VVHYPCEVKMFLGKGDLEYGTGVLDSKIRAHHLSNEMAAFAHQN
                     QLNPLTLSVTFRRVTPTGIEIQPFPFVELRAEAAGLLNFRTKCKSCPVNPSCQPFGCF
                     RSMSYPIPAEIEHWLVSHLEPATTLGGKLFMQAIAWQVELAGIASEMRENGLFELEEP
                     IEWEDPIGNEKVQFNSNFLMGLLFANNQPLTPMMSASILLWFGALKIDGDHPWHLDVK
                     EVEELLLEMDTIESRKQRTALAIDFNQLSTPSLNFMSHWLTALYTGWVESVEVLISA"
BASE COUNT          676 a          612 c          516 g          555 t
ORIGIN      
        1 gggcttcata acttcgataa taaattgttt ccagggcgtc ttcctgagtg acaaattcgc
       61 cgtcaatgac atagccgatt tgccacattc cgtctccgta tttgtggttc cactcgcgct
      121 ggcgggcagt tcgctgtttg atccctccgg cacgtccaat acggcgttcg acaactttcc
      181 agggcataaa aaagagcctc tcaatttttt agatgtgaga ggcccaagca taaaggattt
      241 cagtcaaaac tgacagttag aagaaagaag gagaaccaac acatttacag atgaagtatg
      301 aatgatgaaa ccaactgaac ctcacatcct caccccaggc aaaacctgaa tatatcaagg
      361 acttctatat atgcctgagc ctttagctca gcaaccaggt gatacaattg gctaaatttt
      421 ctcagtcatt catacttcaa tttctaggta cgctcacagg cccgcaactc acgttcggcc
      481 aaaaacccgg cgccaataca gatcaacaca tagatgtgaa agacaaccgc cagagcgctg
      541 cccaggaaag tgtagagcaa cccgtccaac gcatagagcg ctacccctgg tacaaacccc
      601 cagtaggacc gatgacgagc caatacacca aaaaacacaa agagcccggc gaccatcaca
      661 tctaaaaaga ttgacaccac cattccaagc ggaccggtaa acatccgaaa tccagcatca
      721 ataatctggg ttgccgccaa tccaaggaca aagctgacat caccgcccag gtgctgaatc
      781 actgaattga tcaatgacag accagccacc cagaaaaacc agttcccacc attgtgaatc
      841 cgcttgagga gcaattcctg ctttacagga tcaatgagcg tcactgaaac cgggtccggc
      901 tggtatccgg catagtttgc ggaaacaggt tgttgatatg aatagggttg ttgctccata
      961 gcgatggctc ctcaccagat gacaattagt tacatttggc caatgcctgc tgcagaaacc
     1021 tgtcgagacc accagtctca cgcaacgaga gcaactgatc ggcaaacttc accttgagct
     1081 ttggcttgat cactttcata gtggcgtatg ctttgagaat tccatcaacc ccggcccggt
     1141 tcacagccac aagatcactc gcttgatcgg gatgttccac aatgaaccgg gcctggctga
     1201 atatgaagtg accaaataaa aaggtcgatt cgtcagaatc aagatcattt ttctcagtgg
     1261 ctaaaacatc agtacaaacc gtcaccgtca catccggagc atctgccagc caggaaagaa
     1321 cctcgctggc catcttcttt gagtccgaat gatatggatt gctttccatg cgattgatga
     1381 gggcaatcat tttggctttt ttttctggtg aggttggttt ttcggccaaa agcgcagtgc
     1441 tgccagacag caccaaaacc aggcaccacc cccagaccat tcgacgaata aaacgcatga
     1501 tctttctccg aacgttacaa agtgcctgcg atcaggctga aatgagaact tcaacacttt
     1561 caacccagcc tgtataaagc gctgtcagcc agtggctcat aaaattcagg gacggtgttg
     1621 acaattggtt gaaatcaatg gccagtgcgg tccgttgttt ccggctttcg atggtatcca
     1681 tctcaagcaa aagctcttcg acctctttga catccagatg ccagggatgg tcaccatcaa
     1741 tttttaaagc gccaaaccac aacaaaatcg aagcactcat cattggggtc aatggctgat
     1801 tattggcaaa caagagaccc atcagaaaat ttgaattgaa ctggactttt tcatttccaa
     1861 tcggatcttc ccattcaatt ggctcctcca gctcaaagag cccgttttca cgcatttcgc
     1921 tggcaatccc cgcgagttca acctgccagg caatcgcctg cataaacagc tttccaccca
     1981 atgtcgttgc cggctccaga tgtgaaacca gccagtgctc aatttcagcc gggatggggt
     2041 atgacataga tcgaaaacac ccaaatggct gacacgatgg attgaccggg caggatttgc
     2101 atttggtccg gaaatttaag agtccagccg cttcagctcg tagttcaaca aaaggaaatg
     2161 gctggatttc gatgccggtt ggtgtcactc gccgaaaagt gactgacaag gtcaatggat
     2221 tcaactggtt ttgatgggca aaagctgcca tttcatttga gaggtgatgg gcacggattt
     2281 tggaatcgag gactccggtt ccatattcga gatcaccttt tcccaaaaac attttgactt
     2341 cacaaggata gtgaacaac
//
LOCUS       DAOQDO010000926         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40986_length_2357_cov_3.690269, whole genome shotgun sequence.
ACCESSION   DAOQDO010000926 DAOQDO010000000
VERSION     DAOQDO010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40986_length_2357_cov_3.690269"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..390
                     /locus_tag="PKZ53_14400"
     CDS             115..390
                     /locus_tag="PKZ53_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41681.1"
                     /translation="MTHEEMERTMEFILNQQARFSVDIDLLKESQARMSADIASLIEL
                     SRRGMETADADRRLMREMVQDLKDVIYRVESKADEALRKTRNKDVGE"
     gene            complement(482..2140)
                     /locus_tag="PKZ53_14405"
     CDS             complement(482..2140)
                     /locus_tag="PKZ53_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006102163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNJ41682.1"
                     /translation="MPYLILNPGAADNKIFTLKEGNNFLGRARENDIVVLHPSLSRQH
                     ARIDLIGDHAAIMDLQSRNGTFVNGSKIQQSELHDRDQIRCGDAALVFVQGLVSFTQQ
                     DHNIVKQMSPDATRIIMKDLLFEETAKIQAGALKIRTEVSEDRARDKLQILLKVSELL
                     SSPEDIDRLLNKILDLLFQIMDVDRAALLLMDDDTGDLDLKAIKVTARETTQFYSRFI
                     LNYVVEHGVGVLSSDARLDPRFEKAHSIVQQSIRSLICVPLKPRDKVTGVLYVDNLSA
                     SNLYTEEELEFLTGFATQAAIAIDNSKLYKRIESEAVLRNNFMRFFPPKVVQEIAHAQ
                     DLILGAIETEITALFSDISSFTEMSARMEPREMVDLLNAYFPHMAEIVFRYDGTLEKY
                     IGDALMAIWGAPFQHPDDPSRAVRAAIEMQYAVKRFNAEWGSPRGMEIQIHIGLNTGK
                     VAAGNIGSKDYIQYATIGDTTNVTSRICGVAQSHEIMISESTRLKLNDPLIPLEKIDP
                     VKVKGKDEPLQLYRVLWEQVSLEMLEEHLGQTRQADDSSGFSKR"
BASE COUNT          642 a          544 c          584 g          587 t
ORIGIN      
        1 gttaacgcga agaattatag ctaatgcact atttggttat ggttttagca actatcgtgt
       61 gcaaagcgat caatgagaca attgtctaaa gaaatcattt gcggagaaaa caaaatgact
      121 cacgaagaaa tggaacgaac gatggagttc attctaaatc agcaagccag gttcagcgtg
      181 gacattgacc tactgaaaga gtcccaggcc aggatgtcgg cggatattgc gagtctgatt
      241 gaactatcac gacggggaat ggaaacggcg gatgccgata gaaggctcat gcgtgaaatg
      301 gttcaggatt tgaaagatgt catttatcgg gttgaatcaa aagcggacga agccttgaga
      361 aaaaccagga acaaggatgt gggtgagtga actggttatg gattttctgg aaaagaagca
      421 gggaaaaggt aacaggatga caaagtaaca ggtaattttt tcaccctgct tcctttgcta
      481 ttcatctttt cgaaaaccct gaactatcat cagcttgccg ggtttgcccc aggtgctctt
      541 ccaacatttc aagactgact tgttcccaca atacccggta cagttggagc ggctcatctt
      601 tgcctttgac tttgaccggg tcaatttttt caagcggaat caatggatca ttgagtttga
      661 gacgggtgct ttcagaaatc atgatttcgt gactctgggc aacaccgcaa attcgactgg
      721 tgacgttggt cgtgtcgcca atggtcgcat actggatata gtctttggac ccgatattgc
      781 cggcagcgac cttgccggta ttgagtccga tgtggatttg aatctccatt ccgcgcggac
      841 ttccccactc agcattaaac cgcttcacgg cgtactgcat ttcaatcgca gcccgaaccg
      901 cgcgtgaggg atcgtccgga tgttgaaacg gcgctcccca aatcgccatc aacgcatccc
      961 caatgtattt ctcaagtgtg ccatcatagc gaaaaacgat ttcggccatg tgtgggaaat
     1021 aggcattgag taaatcaacc atttcacgcg gctccatgcg agccgacatc tcagtaaaac
     1081 tgctgatatc ggaaaacaga gctgtaattt cggtttctat cgcacctaaa atcaaatcct
     1141 gggcatgcgc aatttcctgc acaactttgg gtgggaaaaa ccgcataaag ttgttacgca
     1201 agactgcttc cgattcaatc cgtttgtaca atttggaatt atcaatcgca atggcggcct
     1261 gggtggcaaa cccggtcaga aactcaagct cttcttcggt gtacaaattt gaggcagaca
     1321 gattgtctac gtagagcacc ccagtcactt tgtcgcgggg tttgagtggc acacaaatca
     1381 atgaccgaat tgattgctgc acaatcgaat gggctttttc aaatcgtgga tcaagtcggg
     1441 cgtctgatga cagcacaccg acgccatgtt caacgacata attcaggata aagcggctgt
     1501 aaaactgggt cgtttcccgg gcagtaactt taatcgcttt gaggtcaagg tcgccggtat
     1561 cgtcatccat cagcagtaag gccgcccggt ccacatccat aatttgaaag agcaaatcaa
     1621 ggattttatt caggagtcgg tcaatgtctt cgggcgaaga aagcaattcg ctgactttga
     1681 gcagaatctg cagtttgtcg cgggcccggt cctcactcac ctcagttcga atttttaagg
     1741 caccagcctg aattttggcg gtttcctcaa acagcaagtc tttcatgatg atccgggtgg
     1801 catcaggtga catctgcttt acgatgttat ggtcttgctg tgtaaaactc accagccctt
     1861 gcacaaacac cagtgccgca tccccacacc gaatctggtc gcggtcatgt aattcggact
     1921 gctggatttt tgacccattg acaaatgtgc cgttgcggct ctgcaagtcc ataatggctg
     1981 cgtggtcgcc aatcaggtca atgcgggcat gctggcgcga taaactggga tgcagcacca
     2041 caatatcatt ctcgcgcgca cgcccaagga agttgtttcc ttccttcaaa gtgaagattt
     2101 tattgtctgc cgcacctgga ttgagaatca aataaggcat tatgaagtcc tggaaacgca
     2161 aacgagcgaa gtcgtgaagt cgtgaacgag cgaagtagaa agaagcgact ccctccactt
     2221 tggctgaacg ggcaggaatc aggcacagaa aaccgggaaa ctggcaaatg ctggatacac
     2281 tgtgtctgaa tcgaagtcaa cctgatttga agggcacgtt gcccaatcaa tgctggccag
     2341 agaaagaaaa atgaatt
//
LOCUS       DAOQDO010000927         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_40992_length_2357_cov_3.119461, whole genome shotgun sequence.
ACCESSION   DAOQDO010000927 DAOQDO010000000
VERSION     DAOQDO010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40992_length_2357_cov_3.119461"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..1896
                     /locus_tag="PKZ53_14410"
     CDS             433..1896
                     /locus_tag="PKZ53_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ41683.1"
                     /translation="MTQSDSYSLPRPAPTLQRILGVGAGLAVSMGVAIGAGIFRNPGE
                     VAGLLPASSLILLVWLAGGILTLIDALVLAELSTFLPKAGGWYVYLRAAYGQFPAFLY
                     GWASSLITYPGSVAAVAVAIGEYFGKLVIPVGHALGLTVTYGPASTRAVAITAIVIFT
                     ILNLTGLRTASWAQQIFTSIKILALLLIVGLAFGLTGNAPPATPAPAVVPSVSTWVAF
                     GIALQFVVWTYDGYADVITLAEEMKQPARDIPRALLGSIGSLTVLYLLLNTAFLYVLT
                     PAEMAASKELVAAEVFARRTGATGELFLVVLAFITVVGGLNAHLLSGPRITFAMARDG
                     LSFAWLSRVTERGTPIGALLLQSTCAVILTLTGTFESLIAMTIFVIWITNALNTFAVF
                     LFRRWYPDAERPYRIPGYPVVPLIALLVSVVLLINMVRETRHQLQEAYPLFAAGHIGQ
                     ALQMLSTSPVLMGIFVVVAGIPVYWLWQRVKRDQVTE"
     gene            1963..>2357
                     /locus_tag="PKZ53_14415"
     CDS             1963..>2357
                     /locus_tag="PKZ53_14415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ41684.1"
                     /translation="MTELTVESHLRVRYAETDQMGVVYHTNYLIWMEIGRTDYCRQCG
                     FQYARMEREEGAFLAVAQVQCRFLASARYDDEIVVRTRLGELRSRQIKFTYTIHRQVD
                     NLVLAEGETVHIVINREGRPMRFPPTYAA"
BASE COUNT          506 a          606 c          629 g          616 t
ORIGIN      
        1 gcacctcttt ttcgaaacga ctgaatgcgg aaagcaattt tagcgtgcac tgtaccaact
       61 aaaccttttg ggctaccgaa ggtagggtgc catcagtcag aagaaccaga accacactgg
      121 acaagagata gttcatcccc tgacaccaaa aattcagatg tacaaaaaag gtgaagctat
      181 cgtcaggtgg gcggaattgc tgtttggaaa agaagacgtg cctgaatctc actgatgctg
      241 cacgagtata ggcacgccaa gcgtggaacg caacccgtaa tttctagttc tggatagtcc
      301 attcctggac agttgctgga tttttctttg atgaaagctg gcgagttaaa cgcatttttc
      361 gggcattact tccccagact tttgattcca ggtactatgc tggccattcc aactttcctt
      421 atttcacttc caatgaccca atctgattca tattcactcc ctcgaccggc cccgaccttg
      481 cagcggattc taggcgtagg cgctgggcta gcagtttcaa tgggtgtggc catcggcgcc
      541 gggatcttcc gaaatccggg tgaagtcgct ggacttcttc ctgcaagcag cctgattttg
      601 cttgtctggc tggctggagg catcttgacg ttgattgatg cgctggtact ggctgaactc
      661 tcaacgttct tgccgaaggc tgggggctgg tatgtgtatc tccgggcggc ctatggtcaa
      721 ttcccggcat ttctgtatgg ctgggcctcc tcgctcatca cgtatccagg cagtgtggcc
      781 gccgtagccg tggcgattgg cgaatatttt gggaaactgg tcattccagt cggtcacgcc
      841 ctgggcctga ccgttaccta tggtccggct tcgaccaggg cggtggcgat taccgccatt
      901 gtgattttta cgatcctgaa tctgacgggc ctgcggacgg catcctgggc acaacaaatt
      961 tttaccagta tcaaaattct ggctttgctg ctgattgtgg gactggcttt tgggttaact
     1021 ggaaacgcgc cgcccgcaac accagctccg gcggttgtgc cttcggtctc aacctgggtg
     1081 gcatttggca ttgcgctcca gtttgtcgtg tggacctatg atggatatgc cgacgtgatt
     1141 accctggccg aagaaatgaa gcaaccagca cgggatattc ctcgggcctt gctcggaagc
     1201 attggaagcc tcacagtgct gtacctgctt ttgaatacag ccttcttata tgtacttact
     1261 ccggctgaaa tggcagcgtc caaagaatta gttgccgccg aggtctttgc ccgccgaact
     1321 ggggccacag gcgagttatt tctggtagtt ctggctttta tcaccgtcgt gggtgggctc
     1381 aatgcccatt tgctgtccgg cccacgcatt acgtttgcca tggctcggga cggcttgagt
     1441 tttgcctggc tcagccgggt caccgaacgc ggaactccaa ttggcgcctt gctgcttcag
     1501 agtacctgcg ctgtgatttt gactctcacc ggaacgtttg aaagtctcat cgcgatgaca
     1561 attttcgtta tctggatcac caatgcactc aacacattcg cggtctttct gtttcgccga
     1621 tggtatccgg atgccgaacg cccttaccga atcccaggct acccggtggt accgttgatt
     1681 gccttgctgg tgtcagtggt cctgctcatc aatatggtgc gcgaaacccg ccatcaactc
     1741 caggaagcct atccattgtt cgcagcagga cacattggcc aggcacttca aatgctctcc
     1801 accagccctg ttttaatggg aatctttgtg gttgtggctg gtattccggt gtactggctg
     1861 tggcaacggg taaaacgtga ccaggtgaca gagtaaaagg accaaaagga cataaattcg
     1921 aaaacccgga acccggaacc cggaacccgg aaccctaaag ctatgactga attgactgtt
     1981 gaatcgcacc tccgggtgcg ttatgccgaa accgaccaga tgggtgtggt gtatcacacc
     2041 aattatttga tttggatgga aataggtcgc actgactatt gtcggcagtg cggctttcaa
     2101 tatgctagga tggaacgtga ggaaggtgcg tttctggctg tggcgcaggt ccaatgccgg
     2161 tttctggcgt cggcccgata tgatgatgaa attgtggttc gcactcgact gggcgaactt
     2221 cgttcgcggc agatcaagtt tacctacaca attcatcgtc aggttgacaa ccttgtatta
     2281 gctgaaggtg aaacggttca tatcgtgatt aaccgtgaag gccggccaat gcgatttccc
     2341 cccacatacg cagccat
//
LOCUS       DAOQDO010000928         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41026_length_2356_cov_3.553672, whole genome shotgun sequence.
ACCESSION   DAOQDO010000928 DAOQDO010000000
VERSION     DAOQDO010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41026_length_2356_cov_3.553672"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKZ53_14420"
     CDS             complement(<1..206)
                     /locus_tag="PKZ53_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41685.1"
                     /translation="MTNFHKQTPPASQAGPPGNVWVAAFRRGPYLWVLLPVCIALIWG
                     VVLPLGALVVESLVDSQARWGIAA"
     gene            complement(214..1872)
                     /locus_tag="PKZ53_14425"
     CDS             complement(214..1872)
                     /locus_tag="PKZ53_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41686.1"
                     /translation="MRESPVILVATDNPSQRALIVSLLQNAKYRVYAASNGYEALAYV
                     QEAVIDALIADISLDGVNGLDVCRQLRAQPTTRHIPILLISPSATRDDDVLSALLAGS
                     DDCLATPFRPQELTLKLRHMLERQHWAVERQRVVENLVWLANSTDLFSSGEQPKYEPP
                     PEPTPNGKPATADDTAELPTPTELFRQRPSETEWDEVEDAMQQALRYFQEVHSGEEEV
                     LIRSAMSRLSTAVAQASLYRQARSHVAGLQEANEKLKELDRLRSEFTNAIVHDIRSPL
                     GSVISTLELIEMELQGKKPDMGELRELINGARQVSEKMISLINELLDFSKMEAGVLQL
                     EFVPIAPHEIIQEAGETWAVVSKRKNVSLSYGCDTNLPKVMADARSIQRALMNLLSNA
                     IKFTPEGGQIWIEARLVEGQQVDAGYSYVVISVIDTGEGIPAQELPYIFDPYYQARSR
                     TGSIGTGLGLAIVKRIAAAHGGNVSVRSQVGVGSAFSIVLPVAMPSVPKSEPQPEFSV
                     DDTNILQAEPSAIKDSSVLSNSSRSVTREIALGLQPHSEVNPIG"
BASE COUNT          602 a          613 c          611 g          530 t
ORIGIN      
        1 taggctgcaa ttccccatcg tgcctgcgaa tcaaccaggc tttccaccac cagggcgcca
       61 agtggtaaca ccacacccca aatcaaggcg atacagaccg ggagcaaaac ccacaaatac
      121 ggcccacggc gaaatgcagc aacccagacg ttccctggtg gtccagcttg agaggcagga
      181 ggggtttgtt tatgaaaatt tgtcacaact ctttcaacct attggattta cttcggagtg
      241 cggctgtaac cccagggcaa tttcccgagt tacagaccgc gaagagtttg acaatacaga
      301 ggaatctttg attgcggaag gctcagcttg taaaatgttt gtatcatcaa cactaaactc
      361 aggctgaggc tcagattttg ggactgaggg catcgccacg ggcaggacaa tggaaaaggc
      421 acttccaacc ccgacctgac tgcggaccga gacattcccg ccgtgggcgg cagcaatgcg
      481 tttgacgata gccagcccca accccgttcc aatagagcca gtgcgactcc gggcttgata
      541 atacgggtca aaaatatagg gcaattcctg ggcggggatt ccttcaccgg tatcaatcac
      601 gctaatcacc acatagctat acccggcatc aacttgctgg ccttccacca ggcgggcttc
      661 aatccagatt tgccctcctt caggagtaaa cttaatcgcg tttgaaagga ggttcatcag
      721 cgcccgctga atgctgcggg catcggccat tactttcggc aaattggtat cgcatccata
      781 actcaatgaa acatttttgc gtttggacac cacggcccag gtttcaccag cttcctgaat
      841 gatttcatgc ggagcaatcg gaacaaattc caactgcaaa accccggctt ccatcttcga
      901 gaaatcaagc aattcattga tcaaactgat cattttctct gaaacctgac gggcgccgtt
      961 gatcaactcg cgtaactcgc ccatatctgg ctttttgccc tgcaattcca tctcaatcag
     1021 ttcgagcgtt gaaatcacgc tccccagcgg cgagcgaatg tcatgaacaa tggcattggt
     1081 aaattcactc cgcagacgat ccagttcctt gagtttttca ttggcttcct gcaagccagc
     1141 cacgtgcgac cgggcctgac ggtacaaact ggcctgggct acggcggttg acaaccgtga
     1201 catagctgac cggatgagta cctcctcttc accgctgtga acttcctgaa aatacctgag
     1261 cgcctgctgc atcgcgtctt caacttcatc ccattcggtt tcagaaggac gctgtcgaaa
     1321 aagttcagtt ggggttggaa gttcggcggt atcatcagcc gtcgcgggtt ttccattggg
     1381 cgttggctcg ggcggaggct catatttggg ttgctcgccg cttgaaaaca gatcagtact
     1441 gttggccagc cagaccagat tttcgaccac ccgctgccgc tcaacagccc agtgttgacg
     1501 ttccagcatg tgtctgagtt tcagggttaa ctcctggggt cgaaatggcg tggccaggca
     1561 gtcatcagaa cctgccagta atgccgaaag cacatcatca tcacgggtgg cagatggact
     1621 gattaacaga atcggaatgt gacgggttgt gggttgggca cgtaactgac gacagacatc
     1681 cagcccattg accccgtcga gagaaatatc agcaatcagc gcatcaatga ctgcttcctg
     1741 aacataggct aacgcctcat atccattgct ggcggcatag acccggtatt tggcgttttg
     1801 gagcagcgac acgatgaggg cacgttgact ggggttgtca gtcgctacga gtatgacagg
     1861 actctctcgc atacggggtt cccctgaagc aggtaaaaac cattgcccgc aaattccaaa
     1921 caacggcatt caagtgctag gatgaaaaag tgtttggccc tggaactcgc ttgaggaaaa
     1981 actctgaaga aatgtgactt tagccggcac gaaacaaggc tgtcaaggaa ggtcatctgg
     2041 aattgaaatc tgaagattct gaaaaatcga aactcctcct gactcattca aatttgattg
     2101 aaccagtgac cccagatatc cagcaaaaaa aacggcgagc aaagaaaact tgctcgccgc
     2161 atagtccaga ctggtattag ctcacccggg ccgaacagta ggagtgtgat gttacaccag
     2221 tattgccctg aggttatttt ttggtgaaaa ccggcagaaa ccagggctga agaaggcggg
     2281 ctgaagacat tgggctgggg gctgggggct ggaagacatt gggctggggg ctgaagacat
     2341 tgggctgggg gctggg
//
LOCUS       DAOQDO010000929         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41057_length_2355_cov_3.616957, whole genome shotgun sequence.
ACCESSION   DAOQDO010000929 DAOQDO010000000
VERSION     DAOQDO010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41057_length_2355_cov_3.616957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..>2355)
                     /locus_tag="PKZ53_14430"
     CDS             complement(361..>2355)
                     /locus_tag="PKZ53_14430"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41687.1"
                     /translation="VSYGKTDGMRTNQCNGGSQPSAWKTRNGKLWFPTAKGAVVIDPM
                     SIKLNQLAPPVVIETVVQGSEKFAPSHFLTFEPGENRFEFRYAGLSYLAPEKVRFKCK
                     LEGFDPTWVDVGTRRVAYYTNLPARTYTFRVMACNNDGVWNEVGTSFTFRLKPPLWQT
                     WWAYTIYLLTVLIIIYASVQWRLNQLHQRAILLESKVHERTQELAQANRQLQSSQEET
                     LWKNEQLLVANQEVERKNEQLDRKNRELDTKIAELAKKNEELLASQKQADRIFSALAE
                     ALPGTVLDGKYQLDEKIGSGGFGAVFRATHLGLNRPVAIKVFKPKPGNDSPEAIERFR
                     REGISTCRVNHPNAITVLDSSVSSEGIAYLVMELLEGHTLTSELQRVGRLSLRRCAEI
                     ILPVCHVLDEAHRAGIIHRDIKPDNIYLHQTKEGEVVKVLDFGIAKLMDDTDESTLRN
                     LTETGGIVGTPTYMSPERLKGDDYDGRADVYSLGIVLYEMLSGRVPFQPSSRGVVEVM
                     LKHLNHQPPSLRDTLPDLPSSVEFVVMTSLRKVATDRPNAIEFGNLLSRAVAESLGVD
                     QEIFYRSGPITAPLPLPTQQLDSQQTNYHHLAHAETLQGAFTLEDHPTIVGNQWAEDD
                     TPTIISHQPVTTNLISEEASERTTERKLTGKLLPQEETLT"
BASE COUNT          508 a          594 c          644 g          609 t
ORIGIN      
        1 gtgcccgtac attttcagtg gctttggcca gaaccaaaag caggtcatcg tcgcttgaat
       61 ccagtagcgt cgtactggcg cggttacctt caatctcggc gattcgagtg cggtcagaca
      121 gagcgtcagg cggtgccggt tgcttaccca taaaaacctc ctggttgttt cctttgggac
      181 aagcacacca ccgggttgac cctcagcggt taacctgccc acagttgagt gagttcaatc
      241 atcgtgcaaa tagtcgttgc ctccattctg aaaaagttca atttgggaaa aactggattg
      301 ttgaacagaa aggtagcgag acagaaaggg ctgatatgtt accgcgctca ggcggtcgtt
      361 tcaagtcagt gtttcttctt ggggaagaag ttttccagtc agtttgcgtt ccgtggtgcg
      421 ttctgatgct tcttctgaaa tcagattggt tgttacaggt tggtgactga tgatggttgg
      481 cgtatcatct tcagcccatt ggttccccac aatggtcggg tgatcctcaa gcgtaaacgc
      541 tccctggagt gtttccgcat gggccagatg gtgatagttt gtttgctggc tgtccagttg
      601 ttgagtcgga agcgggagtg gcgccgtaat cgggccgctt cgataaaata tttcctgatc
      661 aacccccagg ctttcagcta cggccctgga cagtaagttc ccaaattcaa tcgcattggg
      721 gcgatcagtg gcgactttgc gaagcgaggt catgacgaca aactcaacgc tggacgggag
      781 atctggcagg gtgtcgcgta acgatggcgg ctgatgattc aggtgtttga gcatcacttc
      841 gaccacgcct cgactcgaag gctgaaacgg cacccgtcca gagagcattt catacagcac
      901 aatgcccagg ctgtatacat cggcccgtcc atcatagtcg tcacctttga ggcgttctgg
      961 tgacatatag gttggggtac ccacaattcc accggtttcg gtcagattcc ggagggttga
     1021 ttcatcggtg tcatccatta acttggcaat gccaaaatcc agcactttga ccacttcgcc
     1081 ttctttggtt tgatggaggt aaatattgtc aggcttgatg tcacggtgaa tgattccggc
     1141 ccggtgagct tcgtcaagta cgtgacacac cggaagtata atttcggcgc atcgtctgag
     1201 cgagagccgc ccgacgcgct gcaattcgct ggtcagggtg tggccttcca gcagttccat
     1261 caccagatag gcgatgcctt cggatgaaac actcgaatca aggacggtaa tggcattggg
     1321 atgattgacc cggcaggtcg aaattccttc gcgacgaaac cgctcaatgg cttccggtga
     1381 atcatttcca ggtttgggct taaagacttt gatggccacc ggacggttga gtcccaggtg
     1441 ggtggcccgg aaaacagcgc caaatccacc acttccgatt ttttcatcga gctggtattt
     1501 gccgtcaagc acggtaccgg ggagcgcttc ggccagcgct gagaaaatac gatctgcctg
     1561 cttttgcgag gccagcaatt cttcgttctt tttggccagt tcggcaattt tggtatccag
     1621 ttcacggttc ttgcggtcca gttgctcgtt tttgcgctca acttcctgat tggcgaccag
     1681 cagttgctca tttttccaca aggtttcttc ttgtgaactt tgcaactggc gattggcctg
     1741 ggccagttcc tgggtgcgtt cgtgcacctt gctttcgagc aagatcgccc gctggtgtag
     1801 ctggtttaag cgccactgca cgctggcata aatgatgatc agaaccgtga gcaaatagat
     1861 ggtatatgcc caccaggttt gccatagcgg cggctttaat cgaaaggtaa aactggtgcc
     1921 gacttcgttc cacacgccat cgttattgca cgccatcacc cggaaggtat aggttcgggc
     1981 gggcaaattg gtgtaatagg ccacacgccg cgttccgaca tccacccagg tggggtcaaa
     2041 gccctcaagc ttgcatttga atcgaacttt ttccggcgcc aggtagctaa gcccggcata
     2101 gcgaaattca aaccgatttt ctccgggttc aaaggtcagg aaatgggacg gtgcaaattt
     2161 ttcagaaccc tgcaccacgg tctcgataac aacaggcggt gccagttgat ttaatttgat
     2221 agacatggga tcaatgacca cggcgccctt ggccgttgga aaccagagtt tgccgttgcg
     2281 ggttttccag gctgatggct gtgacccacc attgcactgg ttggtccgca tgccatccgt
     2341 tttgccgtag gacac
//
LOCUS       DAOQDO010000930         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41219_length_2350_cov_2.844880, whole genome shotgun sequence.
ACCESSION   DAOQDO010000930 DAOQDO010000000
VERSION     DAOQDO010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41219_length_2350_cov_2.844880"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKZ53_14435"
     CDS             <1..242
                     /locus_tag="PKZ53_14435"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNJ41688.1"
                     /translation="YWLIGLEIFLELFGAREGNGFRRFIHAVTAPVLAPFRQLFYDPS
                     IGPFQLRFSYVAALIVYALLHWFIKRTTRLIPMSG"
     gene            complement(223..>2350)
                     /locus_tag="PKZ53_14440"
     CDS             complement(223..>2350)
                     /locus_tag="PKZ53_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41689.1"
                     /translation="QTEVEITSAPLRQLGDSEIMTVAGGMRAPVVGGNLSTRPRVRLS
                     PQKLIIDQKGNLCFLNGHSELNRIDAISEIITTIIGNHFYPDSVPFGPIPAYSGGDAL
                     LTPIYASDLAQDPEGNLYVAETILHGSGQPGDGYFNLGFILRVDAVTGKITVVAGSSR
                     PTAPSNLENVSPLEVRISPGSMAFDTGGNLYFTDRRTVRCLNRKTGLMSRVAGNGNPQ
                     GDSGDGGPATEARLGQPGSLAFDPEGNLLIADFELNRVRRVDHRTGIISTLVGLSKPG
                     ELQDGTATEGGFSGDGEAAAYAKVNGPISIACDRQGNIYIADFGNDRVRRIDALTGII
                     TSVAGNGNPDHSSGVDLGDGNPATGAQLYRPEGVAIDPQGNLVIADTLNGRIRRVDRQ
                     TGIITTIAGGSTQPLPDENRLATSFGFGDITDIGTDGNENLFILCDKGRDVIRVDALT
                     GTVKTVASFIQGTALAVDRDGNIYCSEADGQILRIDAQTRAVIAFPTNLKHITGLTCH
                     PNGTLLIANGALWSLDITQGTLRQIGGSQEAESENADNIPVQKAQFRATDVALDHLGN
                     LYIADQHFHRIRRIDAQLKFISTIGGNGNPGFSGDGGLAVQAALGEVHSLGVDFLGNL
                     YFSDHSRVRVIDSRTGIITTLAGNGGLLSLITRQGDVGEYDLGLARESSFHSEAITVT
                     PGGNVLIADSRWRVRLTKGIANRSLE"
BASE COUNT          554 a          629 c          561 g          606 t
ORIGIN      
        1 tgtactggct gattggactg gaaattttcc tcgaactctt tggcgcccgc gaaggcaacg
       61 gcttccggcg gtttatccat gccgtgacag cgccggtgct ggcaccgttc aggcagttgt
      121 tttatgatcc gtcaattggg ccgtttcaac tccggttttc ctacgtcgcg gcgctgattg
      181 tgtatgccct gctgcactgg tttattaagc ggacaacccg acttattcca atgagcggtt
      241 agcaattcct tttgtcagcc ttacccgcca tcggctatcg gcaatcaaca cgttcccacc
      301 tggagtgact gtaatcgctt ctgaatgaaa ggaactctcc cgagccaatc ctaaatcgta
      361 ttcgccaaca tctccctggc gggtaatcag gctgagcaac cctccatttc cagcaagtgt
      421 tgtaatgatt ccagttcttg aatcaatcac tcgaactcgt gagtgatcag aaaaatacag
      481 gttgcccaga aaatcaactc ccagtgaatg aacctcacca agtgcggctt gtactgccaa
      541 tcctccatca ccactaaacc ctgggttccc gtttcctcca attgttgaaa taaacttcag
      601 ttgggcatca attcgccgga ttcgatgaaa atgttggtca gcaatataca ggtttcccag
      661 atgatcaagc gccacatccg tagcccgaaa ttgagccttt tgaactggaa tattatcggc
      721 attttctgat tcggcttcct gacttcctcc aatttggcga agggttccct gggtgatgtc
      781 gagtgaccac agcgctccgt tggcaatcag aagtgttccg tttggatgac acgtaagccc
      841 ggtaatatgt ttcagattgg ttgggaaagc gattacagct cgggtttggg cgtcaatccg
      901 gagaatttgt ccatccgctt cgctgcaata aatatttcca tcccggtcta cagccagggc
      961 cgttccctga atgaaggacg cgactgtttt gacggttcca gtgagggcat caacccgtat
     1021 gacatccctt cccttgtcgc aaaggataaa caggttttcg tttccatcag taccgatatc
     1081 ggttatgtca ccaaagccaa aactggttgc aagccggttt tcatcaggta atggttgggt
     1141 tgaaccacca gcaatcgttg tgatgattcc tgtttggcga tccactctgc gaattcgacc
     1201 atttaaagta tcagcaatga caaggtttcc ttgcgggtca atggcgactc cctctggccg
     1261 ataaagctgg gctcctgtag caggatttcc atctcccaga tccacaccgg acgaatggtc
     1321 tggatttcca ttgccagcaa ccgaggtaat gattccggtg agtgcgtcta tgcggcggac
     1381 tcggtcattt ccaaagtcgg cgatgtagat atttccttgc cggtcacaag cgatactgat
     1441 tggcccattc acttttgcgt aagctgcagc ctcaccatca ccggaaaacc ctccttccgt
     1501 agctgtgccg tcctggagtt cccctggttt ggagagtcca accagggtgg aaatgattcc
     1561 tgttctgtgg tccacacggc gaacccggtt cagttcaaaa tcagcgatca acaaatttcc
     1621 ttcgggatca aacgccagac tgccaggctg tcccaatctg gcttctgttg ccggtccacc
     1681 atccccggaa tccccttggg gatttccatt tccagccacc ctggacatca agccggtctt
     1741 tcggtttaga catcggacag ttcgtctatc tgtgaaatac aggtttcccc ctgtgtcaaa
     1801 agccatactg ccaggagaaa tccgaacctc cagcggcgaa acattctcca gatttgatgg
     1861 agcagtaggg cggctgcttc cggcaacaac cgttattttc ccggttacgg cgtccacccg
     1921 aagaataaac ccaagattga aatacccatc accaggttga ccactgccat gcagaatggt
     1981 ttcggctacg tagagattcc cttcaggatc ctgggctaaa tcggaggcgt agattggagt
     2041 cagcaaggca tccccaccgc tgtaagccgg gatcggaccg aacggaaccg aatctggata
     2101 gaaatgattc ccaatgatgg tggtaatgat ctctgatatg gcgtctattc gattcagttc
     2161 agaatgacca ttgaggaaac agaggttacc cttttgatca ataatcaact tctgaggact
     2221 gagtctgacc cgtgggcggg tagacaaatt tccgcccaca actggcgcgc gcattccacc
     2281 agcaacagtc ataatctcag agtcacccag ttggcgaagt ggagccgatg taatttcaac
     2341 ctcggtttga
//
LOCUS       DAOQDO010000931         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41221_length_2350_cov_2.481481, whole genome shotgun sequence.
ACCESSION   DAOQDO010000931 DAOQDO010000000
VERSION     DAOQDO010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41221_length_2350_cov_2.481481"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKZ53_14445"
     CDS             <1..473
                     /locus_tag="PKZ53_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41690.1"
                     /translation="LTSEHQQRAQLETQLAQAREKSNPLPSSESGPGTVASFILLPTL
                     TRGTDDVPPLTLAPSVQRVHFQLVIVGNPSSRLFDVSLQTVEGKKLWGKTKVKAIQKG
                     VKLSVAVELRAEDLSPNDYVLNLDSHNEEKETIDHFEYSFSVLAPSNSKNEPGK"
     gene            591..1490
                     /locus_tag="PKZ53_14450"
     CDS             591..1490
                     /locus_tag="PKZ53_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HNJ41691.1"
                     /translation="MEVLRTPDHRFENLPAFPFLPNYLEVNELRVHYLDEGPRDGQIV
                     LLLHGEPSWCYLYRKMIPVFTAAGLRVIAPDLIGFGRSDKLVRREDYSYQFHIEILKT
                     FIEQLGLTDITLFCQDWGGLIGLRVVAEIPDRFARIVAANTALPTGDHPMPEAFLQWQ
                     HFSQTTPVFPIGTILNRGCVSELSQEVIAAYDAPFPDDSYKAGARVFPSLVPTRADDP
                     EGLRNQQAWEVFKQWNKPFLTAFSDKDPITRGADVVFQKLIPGAKNQPHTTISEAGHF
                     LQEDKGEELAEVVVKFIFPSARA"
     gene            1549..>2350
                     /locus_tag="PKZ53_14455"
     CDS             1549..>2350
                     /locus_tag="PKZ53_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41692.1"
                     /translation="MMKLPLFFQHRLIPNAVGLLAFSALAQSPRNTLSSHWEVFFLAA
                     VPFGIWLGLNYFFWFKPNLKKHPPEDRAKWWYYLADIGSLLAGLVVITFLATMIPPVR
                     RAVARYDHRLVNGLTSYYGGIGFAQRGDLYTRQLGPVLLRDTSVTSRRMQYLNPDEID
                     LISERAEWSLYFAAMRLQGLTTEQIWDLPEGKHLILGTVVAELFPPYWWNQAGWLQQE
                     LKTSPQAEFKLGLIDPQFAKTRLAQFFQTPTSNSQEELRALVFLVSRSP"
BASE COUNT          554 a          586 c          602 g          608 t
ORIGIN      
        1 aattaacctc cgaacatcag cagcgagccc agcttgagac ccagcttgct caagcgaggg
       61 aaaaatcaaa ccctctccct tcctctgaga gcggcccagg taccgttgcc tcatttattt
      121 tgttaccaac gctgacacgc ggaacagatg atgtaccacc gctgacactg gcaccttccg
      181 tacaacgggt tcattttcaa cttgtgattg tcggcaatcc ctcctccaga ttgtttgatg
      241 tcagcctgca aacagttgaa ggaaaaaaac tttggggtaa aaccaaagtg aaggcgattc
      301 aaaaaggtgt caaactctct gttgccgttg agcttcgagc tgaggacctc tccccgaatg
      361 attatgtgct caatcttgat agccataatg aagagaagga aactatcgat cattttgaat
      421 atagtttctc ggtgctggct ccatccaact ccaaaaatga acctggaaaa taagcttcca
      481 aacttgagaa ataaaaataa gatcagtgcc aataagtctc cccaggagta ttgtttctgg
      541 gattgcaaac ccctgttcat cattcccacc atctgaaagg ttaatcttga atggaagttc
      601 ttcgtacacc tgaccatcgc tttgaaaatc tccctgcgtt tccgttttta ccaaactatc
      661 tggaagtgaa tgagttacgt gttcactacc ttgatgaagg acctcgtgac gggcaaatcg
      721 tacttctgct gcacggtgag ccgtcgtggt gttatctcta tcggaaaatg attccagtct
      781 tcacggcggc gggcctgcgg gtgattgctc cggatttgat cgggtttggg cgttcggaca
      841 agctggttcg gcgcgaggat tattcctatc agtttcatat cgaaattctt aaaacattca
      901 ttgagcaact tggtctgact gatatcacac tcttttgtca ggactggggc gggttgattg
      961 ggctacgggt ggtggccgag atccctgatc gatttgcccg gattgtggcg gccaatacgg
     1021 cgcttccgac gggtgaccat ccaatgccgg aagctttttt gcaatggcag catttctcac
     1081 agacaacacc ggtctttccg attgggacga ttctcaaccg gggctgtgtc agcgagcttt
     1141 cccaggaggt gattgcggcc tatgatgcgc cgtttccaga tgattcctac aaggccggcg
     1201 cccgagtgtt tccaagtctg gttccaaccc gcgctgacga tccagaagga ctcaggaatc
     1261 aacaggcgtg ggaagtgttc aaacaatgga acaagccgtt cctgacagct ttcagcgaca
     1321 aagaccccat tactcgtggg gccgatgtgg tctttcaaaa gttgattccc ggcgccaaaa
     1381 atcagccgca cacaacgatt tccgaagccg gacattttct tcaggaggat aaaggtgagg
     1441 aactggcgga ggtggtcgtg aagtttattt ttccgagtgc gagggcttga cgtcgttttg
     1501 gaatgaaaaa gcggtgtcaa gccaccgcac tccatcgccg aaaaataaat gatgaagctc
     1561 ccattatttt tccaacaccg gctgattcca aatgcagtcg gactcctggc cttttctgcg
     1621 ctcgcccagt caccccgcaa taccctgagc agtcactggg aagtcttctt tcttgctgcc
     1681 gtgccgtttg ggatctggct ggggctgaat tattttttct ggtttaagcc gaatctcaaa
     1741 aaacatccac cggaagatcg ggcaaagtgg tggtattacc tggctgatat cgggtcgttg
     1801 ctggctggat tggtcgtgat aacttttttg gcaaccatga tcccgccggt tcgcagggcg
     1861 gttgcccgct atgatcaccg actggtaaac gggttaactt catattatgg cggcatcgga
     1921 ttcgcccaac gtggagattt gtacactcgt cagcttgggc cggttttgct ccgtgatacc
     1981 agcgtgacca gccgtcgaat gcagtacttg aatccggatg aaattgacct gatttccgaa
     2041 cgggcagagt ggtcgcttta ttttgccgcg atgcggctcc aggggctgac caccgaacaa
     2101 atttgggatt tgccggaggg caaacacctg attcttggga cagttgtggc tgagctgttt
     2161 cctccctact ggtggaatca ggccgggtgg ctccagcagg aactaaaaac ttcaccgcag
     2221 gcagaattca aactggggct cattgaccct cagtttgcga agaccaggct ggcgcaattt
     2281 tttcaaactc cgacttcaaa ttcccaggag gaactccggg cgctggtgtt tctggtcagt
     2341 cgaagtcctg
//
LOCUS       DAOQDO010000932         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41254_length_2349_cov_2.666521, whole genome shotgun sequence.
ACCESSION   DAOQDO010000932 DAOQDO010000000
VERSION     DAOQDO010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41254_length_2349_cov_2.666521"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1392)
                     /locus_tag="PKZ53_14460"
     CDS             complement(49..1392)
                     /locus_tag="PKZ53_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNJ41693.1"
                     /translation="MDYEIALASITLVVLVFLSIVESAHGCLSDLALRSLASEAHSAH
                     PARARFLRYLLEHRQLFWLTLSFGIQAAIILLTLVLADLMGQLQIVPVGFGIPVVFVA
                     TVTLVLIFRQFLPRLLVQNSPGPFLMRLLPVFEPYFRVTALFVFPVHEILKTFRESQD
                     LSVTPDSFQNTGSDEDLKALIDVGEEEGIIEESDSELIQSIIEFGDTVVKEVMTKRSA
                     MVCIEDSATIEQVRDLMVEERHSRLPVYQGNPDNIVGVVYVRDLLGCWSENQTTELVG
                     VIARPAYFVPENKSVADLLEDMQKSKTQIALVIDEFGGVAGLVTIEDILEEIVGEIED
                     EDVEETSPEELEIVEEAEGWYLVKGSTEIRKIELLFDVELEADDFTTVNGLIIKSLER
                     VPNVGEQFFLRGLHIEILEADGRAIRRVRLQWHDGTESTMANRETEAANSSNFEL"
     gene            complement(1733..2212)
                     /gene="ybeY"
                     /locus_tag="PKZ53_14465"
     CDS             complement(1733..2212)
                     /gene="ybeY"
                     /locus_tag="PKZ53_14465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNJ41694.1"
                     /translation="MISFINRQRREQLEKAVICELARQTAEVLGLSRFEISLVFVGDP
                     VMQALNQQYRGIDKPTDVLSFPMIELPSPYESAAAAQLKRFQVIEGDLLGEVVISTHT
                     ARRYAQELNLTFPVEIKTLIIHGILHLRGYDHETDQGEMERLERKLRKQLITNQIDW"
BASE COUNT          644 a          594 c          529 g          582 t
ORIGIN      
        1 tggtttttgc ccttttgact attttgctat ttcactcttt gacactcact acagttcaaa
       61 attggaagaa ttcgcggcct cagtttcgcg gtttgccatg gttgattcag taccatcgtg
      121 ccattgcaga cgaactcgtc gaatcgcccg tccgtcagct tcaagaattt caatgtggag
      181 cccgcgaagg aaaaattgct ctccaacatt gggaacgcgc tccagtgatt taatgatcag
      241 cccgttgacc gttgtaaaat catcagcttc aagttcgaca tcaaacagta attcaatttt
      301 gcgaatttca gtgctgccct tgaccagata ccagccttca gcttcttcaa ctatttccaa
      361 ttcttcaggg ctggtttcct caacatcttc gtcttcgatt tcgcccacaa tttcttcaag
      421 gatatcttca atggtcacaa gtcccgcgac accgccaaat tcgtcaatga ccagtgcaat
      481 ttgggttttt gacttttgca tatcttccaa caaatcagcg actgatttat tttcaggtac
      541 aaaataggct gggcgggcaa tcacgccgac taactcagtg gtttggttct cactccaaca
      601 ccccagaagg tcacggacat agacgacgcc cacaatatta tctgggtttc cctgatatac
      661 cggcagtcgc gagtgtcgtt cctcgaccat caaatcccgg acttgttcaa tggtggctga
      721 atcctcaatg cagaccatgg cggaacgttt ggtcatgact tctttcacaa ccgtgtcgcc
      781 aaactcaatg atggactgga tgagttcgct atcagattcc tcaatgattc cctcttcctc
      841 gccgacgtca atcagggctt tcaaatcttc atcggagcca gtgttctgaa agctgtcagg
      901 ggtcacactg agatcctggg attcgcgaaa ggttttgagg atttcatgga cgggaaaaac
      961 aaacagggcg gtgacgcgaa aatacggctc aaaaacaggt aacagccgca tcagaaacgg
     1021 cccaggggaa ttttgaacca gcaaccgggg taaaaactga cgaaatatca gcaccagcgt
     1081 cacggtggca acaaacacca ccggaatccc aaatcccacc gggacaattt gtagttgtcc
     1141 catcaggtcc gccagcacca gcgtcaacag gataatggcc gcctgaatac caaatgacag
     1201 agtcagccaa aataactggc gatgttccaa caaatagcgc aaaaaccggg ctctggctgg
     1261 atgcgcacta tgggcttcag aagcaagcga acgcaacgcc agatcgctaa ggcaaccatg
     1321 cgcactctca acaattgaaa gaaacaccag aacgaccagc gtgatgctgg ccaaagctat
     1381 ctcataatcc ataattgaaa aactgggcgg gtacggcccg aaaaacggag tccccaacac
     1441 aagtcagatc tgatcaaaca gcgtggattc ggactttaaa aattcattcg acgatcaatt
     1501 gataccatcc gaaatcctgg aatgaacaat ccgcttgagg ttttgaaact tcaaattgct
     1561 acaggtagtc gaggtgaacg aagatttgcc aaaaccgagg ggcaacaatg gaaatcaatc
     1621 tgcctgggag aatatcatgt tgaggacgag cctggaatga aagcctggag caacggctct
     1681 gattcttcca ggataagaag acccatctca aacctgattg accacacctg ccttaccagt
     1741 cgatctgatt ggtgatgagt tgcttgcgta gttttcgctc aagccgctcc atttcgccct
     1801 ggtcggtttc gtggtcatat ccccgtaaat gcagtattcc gtgaatgatc agtgtcttaa
     1861 tttcaactgg gaatgtcaag ttgagttcct gagcatagcg ccgcgccgtg tgggttgaaa
     1921 taacaacttc tccgaggaga tcaccttcga tcacctggaa tcgcttgagc tgagcggcag
     1981 cggctgattc atagggcgag gggagttcaa tcattggaaa tgacaacaca tcggttggtt
     2041 tatcaattcc ccgatattgc tggttgaggg cttgcattac cgggtcaccc acaaagacca
     2101 gactgatttc aaaccggctc agtccaagta cttcagccgt ttgcctggca agctcgcaga
     2161 tcaccgcttt ttcaagctgt tcacgacgct gacgattgat aaaggaaatc ataaggcggg
     2221 atatagaatg aagaattgag aatgaagaat gaacctggtg cttggtgctt ggtgcttagg
     2281 aaccaatcct ttccaagcac cagtcacccc aaacattctt cattctcaat tcttcattct
     2341 caattcttc
//
LOCUS       DAOQDO010000933         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41275_length_2348_cov_3.582643, whole genome shotgun sequence.
ACCESSION   DAOQDO010000933 DAOQDO010000000
VERSION     DAOQDO010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41275_length_2348_cov_3.582643"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKZ53_14470"
     CDS             <1..515
                     /locus_tag="PKZ53_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ41695.1"
                     /translation="NHGISTGALFLFVGMIYERRHTRLISEFGGLVTPMPLYAVLFVF
                     TCLSSAGLPMLNGFVGEFLILLGSFISQHNVVYTALAATGVILSAVYLLWMLQRVVFG
                     ELTNPKNAKLEDMNTREIVMVAPLIVLMVIMGVAPNLFLRSMHADMEKVQKAVVKVAK
                     ADTQQTKLAH"
     gene            688..2247
                     /locus_tag="PKZ53_14475"
     CDS             688..2247
                     /locus_tag="PKZ53_14475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNJ41696.1"
                     /translation="MFILPELPKLQDYVVIIPEICITLTAFVIMMYDAFSSQDRRRDA
                     GVIALAGIIGAGGGLLRLWNAPEYTAFGGMFVSDQLRLYFVLIFLVVAALAVLASMQF
                     LDDERLPPGEFYTLLMFATVGMMLMAAAGDLATLFLGLETLSITTYVLAGYRRGDVRS
                     NESALKYFILGSFSTGFLLYGIALTYGATKTTNLARIHDAILTGDITSMPLLMMGAAM
                     MLIGLSFKVATVPFHLWTPDVYQGAPTAVTAFMAAGPKAAAFVAFLRVFVEAFAQDTV
                     LFGTSSANTGGNLYSTWTSAVVIIAIMSMIIGNVVAITQTNIKRMLAYSSIAHAGYGM
                     VGILGNDWKPVAFYMLAYAVTSMGAFTVISLLARQGDEKTEIDDFAGIGFQNPGLSVL
                     LMIFLLSLGGIPLTAGFIGKFVVFKQAWFAGYQPVVIIGVLTSAVSLYYYLRPLVVMF
                     FQNREGEYVPPRISFESGVALAAALLLVFGLGDYPEPLLKNKDKEAPAAAAQYQSPVE
                     KGDSAWATASK"
BASE COUNT          487 a          580 c          635 g          646 t
ORIGIN      
        1 tgaaccacgg tatctcgacc ggagcgctct ttttgtttgt cggtatgatt tatgaacgcc
       61 gtcacacgcg cctgatttca gaatttggcg ggcttgtgac cccgatgccg ctctatgcgg
      121 tgttgtttgt ctttacctgt ctttcctcag ccgggttacc catgctcaac ggattcgtcg
      181 gtgaattttt gattctgttg gggtccttca tttcgcaaca caacgtggtt tacacggcgt
      241 tggcggcaac gggcgtgatt ttgtcggcag tttatttgct gtggatgctc cagcgcgtgg
      301 tgtttgggga acttaccaat ccgaaaaatg ccaaactgga agatatgaac acccgcgaaa
      361 tcgtgatggt ggcaccgttg attgttctga tggtcattat gggcgtggca ccaaacctgt
      421 tcctgcgcag catgcacgct gatatggaaa aagttcaaaa agcggttgtc aaagttgcta
      481 aagccgatac tcagcaaacc aaactggccc attagagaaa agcgagtcgc cctgaaggcg
      541 actcagtcag taccacccgc gtcagtgggt gggtcagtcg gctttccaac cccacccgct
      601 cacgcaggtg gtactgacac gtcctttaga agcaggattt tgttaatccg aaattgagta
      661 aagaaatctg actgaaaaaa ctgtcttatg ttcattttac ctgaacttcc caagctccaa
      721 gactacgttg tcatcattcc cgaaatctgc attaccctga cggcctttgt catcatgatg
      781 tatgacgcct tttcgtcaca ggatcggcgg cgggacgcgg gggtgattgc gctcgccgga
      841 atcatcggcg cgggtggtgg tctgctccgg ttatggaatg ctcctgagta cactgccttt
      901 ggcggcatgt ttgtcagcga tcaactacgg ctgtattttg tgctcatctt cctggttgtg
      961 gcagcgttag cggtactggc ctcgatgcag tttttggatg acgagcggct tccaccgggt
     1021 gaattttaca ccttgctgat gtttgccacg gttggaatga tgttgatggc ggctgccgga
     1081 gacctggcaa cattgttttt gggacttgaa acgctttcaa tcacaaccta tgtgctggct
     1141 gggtaccgtc ggggtgacgt tcggtccaac gaatcagccc tgaagtactt cattttgggg
     1201 tcgttttcga ctggcttttt gctctatggc attgcgctga cctatggggc aaccaaaacc
     1261 accaatctgg cgcggattca cgatgccatt ttaacgggtg atattacctc gatgccgctc
     1321 ctgatgatgg gggcggccat gatgctgatt gggttgtcat tcaaggttgc caccgtgccc
     1381 tttcacctct ggacgccaga tgtctatcag ggcgcaccaa cagccgtgac cgcctttatg
     1441 gcggctggtc ccaaagcggc tgcgtttgtt gcttttctgc gtgtttttgt cgaagccttt
     1501 gcccaggaca ccgtgctgtt tggaacgtca agcgctaata ctggcggtaa tctctattcg
     1561 acctggacaa gcgctgtggt gattatcgcg atcatgtcaa tgatcatcgg gaacgtggtt
     1621 gcgattactc aaaccaacat caagcgcatg ctggcctatt cttcgattgc ccacgccgga
     1681 tatggcatgg tcggtatttt ggggaatgac tggaagcctg tggcctttta tatgctggcc
     1741 tatgctgtaa ccagcatggg cgcctttacc gtgatttcac ttctggcccg ccagggcgat
     1801 gaaaagaccg aaattgacga ttttgccgga attggattcc aaaatccggg gctttcagtg
     1861 ctgttgatga ttttcctgct cagtctgggt ggaatcccat tgaccgccgg gtttattggc
     1921 aagttcgtgg tctttaagca ggcctggttt gccggatacc agccagtggt gatcattggc
     1981 gtgctgacca gcgcggtttc gctctattac tatttgcgtc cactggtcgt gatgttcttt
     2041 caaaaccggg aaggcgaata tgtaccaccg cgcatttctt ttgaatccgg tgtggcgctg
     2101 gcggcggcct tattattggt ttttgggttg ggtgactacc ctgaaccact gttgaaaaat
     2161 aaagacaaag aagctccagc cgctgcggct cagtatcaat cacctgttga gaaaggagat
     2221 tccgcatggg caacagcttc gaaataaccg ttgaagaact caaagcccgc cttgatcagg
     2281 gcgacaaacc gttcatcctc gacgttcgcg aacagcacga atatgacatc tgccatctgg
     2341 ccgacacg
//
LOCUS       DAOQDO010000934         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41307_length_2347_cov_3.770942, whole genome shotgun sequence.
ACCESSION   DAOQDO010000934 DAOQDO010000000
VERSION     DAOQDO010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41307_length_2347_cov_3.770942"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKZ53_14480"
     CDS             <1..1065
                     /locus_tag="PKZ53_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNJ41697.1"
                     /translation="ASTHRYEFRPLANGKLTEIYHVNNVQRPFNGTIRKHFVQLIPDV
                     VVAHNRAVDRFFREEAAEYIAFDTQTGDVPEIRQAALKALGERAGTVLVMDPNTGRVL
                     SIVNQEWAVRTSFAPASTIKLVTGLAGLATGAIQPDVKVDLPTENRQCDFREAMALSS
                     TAYFQKAGEKVGQSRFLEMLAQFGLGEMTGINLAGEIPGTVITDPKAAAPRNFFGAAE
                     GMELTPLQMASLVSTIANGGTVYTPQFASSPASFKPVIRRQANLPARELELLKSAMVD
                     VVEVGTASVIRNPKLSIAGKTGTAVKDGLWTGWFVSYAPVANPKLAIVVVLQGADTRG
                     SNAAKVAGQIFSQLQSQLGQ"
     gene            1615..>2347
                     /locus_tag="PKZ53_14485"
     CDS             1615..>2347
                     /locus_tag="PKZ53_14485"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41698.1"
                     /translation="MFEHFKTVTLSLVGFCLAFCLPAVVCGQNREISFQRINQEAGLS
                     NNAIRCFAQDTEGYIWIGTESGLNRYNGISFEVFRHKANDPNSLAFDFVSALYCDRKG
                     RLWVGTQQKGLDLFDPATGSFKHFTDDVFRGPFQRTSVIACITEDSAGKLWIGTQHGL
                     VVFDPTIQSFTRYTLFDWPGDNTVCSVVFDRFGHVWVRTLDHLYLFDLKTARFHLLLD
                     SPMESLARGINNLNTANSRTFRRTRF"
BASE COUNT          558 a          608 c          567 g          614 t
ORIGIN      
        1 gccagtacgc accgatacga atttcgaccg ctggcgaacg gcaaactcac tgaaatctac
       61 catgtgaaca atgttcaacg tccctttaac ggcaccattc gcaaacattt cgtgcaattg
      121 attccagacg tggttgtggc gcacaaccgg gccgtagatc ggtttttccg tgaagaagcg
      181 gctgaataca ttgcctttga tacccaaaca ggtgacgtcc ccgaaattcg ccaggccgcc
      241 ctgaaagccc ttggtgagcg ggcaggaaca gttctggtca tggatccaaa taccgggcgt
      301 gtactgtcta tcgtaaacca ggagtgggcc gtgcgcacca gttttgcccc agcttcaacc
      361 atcaaactcg tgactggatt ggccggactg gcaactggcg ccatccagcc ggacgtcaaa
      421 gtggacctcc caaccgaaaa ccggcaatgt gattttcgag aagccatggc cctttccagc
      481 acagcgtatt ttcaaaaagc aggcgaaaaa gtcggccaat cccgtttcct tgaaatgctg
      541 gcccagtttg gcctgggaga aatgacgggg atcaacctgg ccggtgaaat tccaggcaca
      601 gtcatcaccg atccgaaagc cgccgcgcca cgaaacttct ttggtgccgc agaaggaatg
      661 gagctcacgc cactccagat ggcatctctg gtttcaacca ttgccaatgg gggaacggtc
      721 tatacccccc aatttgcatc ctctccggcc agtttcaaac ctgtcatccg ccgacaggcc
      781 aacctgcctg cccgtgaact ggaattgctc aaatcagcca tggtggatgt agtggaagtt
      841 ggtacggcct ccgtaattcg caatccgaaa ctgagcattg ctggtaaaac cggcaccgct
      901 gtgaaagatg gattatggac cggctggttt gtgtcgtatg ccccggttgc caaccccaaa
      961 ctggcaattg tggtggtttt acagggggct gatactcgcg gctccaatgc ggctaaggtt
     1021 gccggacaga ttttctccca gcttcaaagc cagcttggcc agtagttttt ggccgcgtat
     1081 gaaacactaa cgattgattc acaactggat ctctggctga ctgaataaag gaagcatcaa
     1141 aacaacgaag ttttttttca gttaactgga gttttttttc aatagtattt ggttgctcaa
     1201 tcaagcaaag ggagtaagat ggccggaagt agattccact cataaatttc tccttcacag
     1261 ttacattcag gcgtgcggta gggaactggt gttcagcatc acatcacttc cctaccgctt
     1321 tcttcttttt tccccaaaat agtttgcgct ctgaaatgac tcggtttacc tttgggtttc
     1381 attcgcaatt ccaccggcct tacaccaaat tgctttttga accacacccc ttccaaaaca
     1441 gtgtgaaggt gattgattga ccagaattgg atcccaggcc gtcctgatgt gacctggtct
     1501 ttgtcaaatt caggtcaatc aaccgctttc cacttttttc caggcaaggt gttgttgtgg
     1561 aatgaacctg gattttctct ttccctgccc gggattgaat cacagtcaca tatcatgttt
     1621 gagcacttca aaaccgtcac gctcagcctg gttggatttt gtctggcttt ctgcctgcca
     1681 gcggttgtgt gcggacagaa cagagaaatc tcatttcagc gaataaacca ggaagcgggc
     1741 ctttccaata atgcgattcg ctgttttgcc caggacacag aaggctatat ctggattggc
     1801 accgagagtg ggttaaaccg ctataacggc atttcctttg aggtttttcg ccacaaagcc
     1861 aatgacccaa attcactggc ctttgatttt gtctcggcgc tctattgtga ccgaaaaggc
     1921 agattgtggg ttgggacgca acaaaaaggt cttgatttat ttgacccggc aactggttct
     1981 ttcaaacact ttacagatga cgtttttcgt ggtcccttcc aaagaacctc ggtgattgcc
     2041 tgcatcaccg aagattcagc cggaaagctg tggattggca cccaacacgg actggttgtg
     2101 tttgatccga cgattcaatc cttcacccgg tacaccttat ttgactggcc tggcgacaat
     2161 acggtttgtt cagttgtatt tgaccggttt gggcatgttt gggtccgcac actggatcat
     2221 ttgtacctgt ttgatttgaa aacagcccgg tttcacctgt tgcttgatag cccgatggag
     2281 tctctggcgc gcgggatcaa taatttgaat accgccaaca gtcgcacttt ccgcagaacg
     2341 cgcttcc
//
LOCUS       DAOQDO010000935         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41309_length_2347_cov_3.109948, whole genome shotgun sequence.
ACCESSION   DAOQDO010000935 DAOQDO010000000
VERSION     DAOQDO010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41309_length_2347_cov_3.109948"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PKZ53_14490"
     CDS             complement(<1..1216)
                     /locus_tag="PKZ53_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNJ41699.1"
                     /translation="MLFSFFHPDQDRTVEVESILENQSDLKSALDAVVLEWIPYVATG
                     LCVFYYLVAILHWCFVPPPSSRYLSAVALTSGVLLTAILEAHRRKAIPLAWSQVLGFG
                     IFLLPVFNSLLHLWFVPEAKHTTNLILTLVGSGCLLLSMRDYWVLAGLSLAGWCFIAT
                     QSPPGPDWGHFAFTLCSAVIIGFVVQAARLRTYQRLELLRLKSEVRKKELEVATTQAE
                     QASLAKSEFLANMSHEIRTPMNGIIGMAELLLESQLTPEQRECAETINGSSRALLAII
                     NDILDFSKIEAGKLELENIEFDLRQTIESITNLLAEFAHRKNLELVVQIDPDVPTQLY
                     GDSLRLRQILVNLINNAIKFTTTGQVLVRVQPAPQTDAQQGLIRFEVIDTGIGIPPET
                     LARLFTSFTQADG"
     gene            1388..>2347
                     /locus_tag="PKZ53_14495"
     CDS             1388..>2347
                     /locus_tag="PKZ53_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNJ41700.1"
                     /translation="MSDSTTTSIREWTVDLVRNHCAGAARLELWTIPLYLSVAYSINV
                     VPHPATGRPELTAVNYPVLPDGLPDYNWFQDKPQHAAQFAFNNIMSIAFQEMLHVELA
                     ANLCNAIGASVDFIGDNGAFFPKYDPDSVPFIAVPSDLRELVRLAPLGPDSIALLTWI
                     EHYLPRFPSPPLSTQPRDQYRSLGEFYHALQHGVDVMWEDLYPQQGSPTEADLKQKDD
                     FQTVLHRKRRDWVQEEYQFSMTISGSSHQARKCTDSLIRAIISQGEGATEGNHLQVNP
                     AYQLKHGDATEIALDRWTHWERFSQIEEILKVVQIETFAPQTGS"
BASE COUNT          600 a          587 c          595 g          565 t
ORIGIN      
        1 aaccatctgc ctgggtaaat gaagtaaata accgggcaag tgtttcaggc ggaatcccaa
       61 tcccggtatc aatcacttca aaccggatca ggccctgctg cgcatcagtc tggggagccg
      121 gttgaacccg gacgagaacc tgtcctgtgg tggtaaattt gatcgcgtta ttgattaaat
      181 tgaccagaat ctggcggagc cgtaacgaat cgccataaag ctgggtcggg acatctggat
      241 caatctggac aaccagttcc aggtttttcc gatgggcaaa ttcagccagc aggttggtaa
      301 ttgactcaat ggtttgtcga aggtcaaact caatattttc aagctccagt ttcccggctt
      361 caattttcga aaaatccaaa atgtcattga taattgccag caatgcccga ctggaaccgt
      421 taatggtttc agcacattcg cgttgttctg gggtgagttg tgattccaag agcaattcag
      481 ccatgccgat aataccgttc atgggagttc tgatttcgtg gctcatattg gccagaaatt
      541 cacttttggc aaggctggcc tgttcagcct gagtggtcgc cacttcaagt tcttttttgc
      601 gcacttcgct tttcagtctg agtaattcca gtcgctgata ggttcgcaat cgggcagcct
      661 ggacgacaaa gccgataatt acggcactac acagggtgaa tgcaaaatgt ccccaatcag
      721 ggccgggtgg ggactgagtg gcaataaaac accacccggc gagcgaaagc ccagccagca
      781 cccagtaatc ccgcatcgaa agtagcaaac agccgcttcc aaccagagtc agtatcaggt
      841 ttgtggtgtg tttggcttct gggacaaacc agagatggag cagactattg aaaaccggaa
      901 gcaggaagat tccaaacccg agaacctggc tccaggcaag cgggatggct ttccggcggt
      961 gagcttcaag aattgccgta aggagaacgc ccgatgtcaa ggcgactgct gacagatatc
     1021 gggaagaggg cggagggaca aagcaccagt gcagtatggc caccaaatag taaaaaacac
     1081 ataacccggt ggcaacatac ggaatccatt ccaaaacaac cgcatcaagc gcgcttttca
     1141 ggtcgctttg attctccaaa atggattcaa cttcgacggt ccggtcttga tcaggatgaa
     1201 aaaatgagaa aagcatgaaa cagcctgttg gcagaaataa aatgagagat agaatgcctg
     1261 ggagtgtact ctaattacca gcctttgtga cctctcgctt gccgccaaag gtcactgatt
     1321 tccgatcatc atccaggcgt tttgaccgca atctttctgt cttttttctg aaaaggagct
     1381 tgaattcatg agtgattcca ccacaaccag catccgtgaa tggacggttg atcttgtcag
     1441 aaatcattgt gcgggggctg cccgccttga actatggacc attcccctgt atttgtccgt
     1501 ggcgtattca atcaacgtgg ttccacatcc tgccaccggt cgcccggaat taacggcggt
     1561 gaactacccg gttttgcccg atggattgcc cgattataac tggtttcaag ataagcctca
     1621 gcacgcagcc cagtttgcct ttaacaacat tatgtccatt gctttccagg aaatgttgca
     1681 tgttgagctg gcggcgaatc tgtgcaacgc gattggggcc agcgttgact ttattggcga
     1741 caatggcgct ttttttccaa agtatgatcc tgattcggtt ccttttattg cggtaccttc
     1801 tgatctcagg gaactggtcc ggttggcccc acttgggccc gattcaatcg cgttgctgac
     1861 ctggattgag cattacctgc cacggttccc ctcacctccg ctcagtaccc agccccgaga
     1921 tcaatatcgt tccctgggcg agttctatca tgcgctccag cacggggtgg atgtgatgtg
     1981 ggaagatctc tatccacaac agggcagccc aacagaggca gacctcaagc aaaaagatga
     2041 ttttcaaacc gtactgcatc gcaaacggcg cgactgggtg caggaggagt atcaattcag
     2101 catgaccatt tccgggagtt ctcaccaggc gaggaaatgt actgattctc tgattcgggc
     2161 tattatcagc cagggcgaag gggccaccga aggcaatcat ttgcaggtca acccggccta
     2221 ccagctcaaa cacggcgatg cgactgaaat cgcgcttgat cggtggacgc attgggaacg
     2281 cttttcacaa atcgaagaga ttctaaaagt ggttcagatc gagacatttg ccccccaaac
     2341 aggttct
//
LOCUS       DAOQDO010000936         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41335_length_2346_cov_3.449149, whole genome shotgun sequence.
ACCESSION   DAOQDO010000936 DAOQDO010000000
VERSION     DAOQDO010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41335_length_2346_cov_3.449149"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="PKZ53_14500"
     CDS             complement(<1..1965)
                     /locus_tag="PKZ53_14500"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNJ41701.1"
                     /translation="MSVSSRHSARFRLVALFLFLALPSLSFGLLTSLTSKSAGPAHHQ
                     SQIQSELLPATIQSSESQPQVIPGPEGSFFRVDGHVLVQVLPNGGRSVWHEFSETEAV
                     NAQLLEIGGTLFGTTQTGGAQNLGTLFGFNPQTGEVMTIWNFGGLDGARPAGNLIADR
                     QGNIYGTTLNGGEDDNGVVFRIKLDGTGYKPVFHYLKSDGYDPTAGLILLADGSVTPV
                     INPTEPENQPVESQRGEFQPAATFTVTTAAELVTAINTANTNSQADIINLTANIGLTS
                     ALPTLASNGGNSTTINGGGFTVFRAGGSFRIFTISNGAVVIMNNMTISGGSTGAFGGG
                     ILNQGNLTISNCRISGNSGARGGGIRNENLLTMTDCTVSGNTSGEGGGAGFEFGTSTI
                     SGCTFTGNIQTDGGAGSGGGVSHTLGGNVFLTNCTITGNTAASFSGAAGVSIILGSGS
                     TNLANCTITSNTTSGPGGGIRNFNGALNLRNTIIFGNTSNEIDGGVSFNANNLVGSNG
                     TGGISNGVNGNIVGSNPVLGPLASNGGPTQTQAIGCASPARDAGTTVGTPTTDQRGVT
                     RDTAPDIGAFEGPLVVSIGSPTPPSFDSPGSVSFPVTYVGAASVNLTNAAITVNTTGS
                     VVGTANVTNGTTATPTVTVTVTSGVGTVGIS"
     gene            complement(2133..>2346)
                     /locus_tag="PKZ53_14505"
     CDS             complement(2133..>2346)
                     /locus_tag="PKZ53_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41702.1"
                     /translation="LQDGLTGTRQILDFDPKAVVVVVTKYRDERIREKARQAGACNLL
                     EKENLLRLTELVRFTLGLTHNLNPGS"
BASE COUNT          577 a          624 c          592 g          553 t
ORIGIN      
        1 tgaaattccc accgttccaa cgccgctcgt gaccgtgacc gtgaccgtcg gcgtcgcagt
       61 ggtgccattg gtgacattgg cggttccaac gacactgcca gtggtgttca ccgtaatcgc
      121 ggcatttgtc aggttgaccg aagcggcacc gacataagtc acagggaaac tgacggaacc
      181 tggggagtca aagctcggag gcgttggtga tccaattgag acgacgagcg gtccttcaaa
      241 cgcgccgatg tcgggcgccg tgtcgcgggt cacgccacgc tgatcagtgg tgggtgtacc
      301 aacggttgtt ccggcatcac gtgccgggct ggcacaacca atggcctgag tctgggttgg
      361 accaccattg ctggcgagtg gtccaagcac gggatttgat ccgacaatgt ttccattgac
      421 gccattggag atacctccag tgccgttgct tccaaccaga ttattggcgt tgaatgacac
      481 tcctccatca atctcattgc tcgtatttcc aaaaataatg gtgtttctca gattgagtgc
      541 gccattaaaa ttccgaattc caccgccagg accactggtg gtgttgcttg tgatggtaca
      601 gtttgccagg ttggttgaac cagatccaag aatgatgctg actccggcgg cgcccgaaaa
      661 tgatgccgcc gtgttgccag tgatggtgca atttgtcaaa aacacatttc cgccaagggt
      721 atgagaaaca ccacccccgc ttccagcgcc cccatcggtt tgaatattgc cggtaaaggt
      781 acaacccgaa atagtggacg ttccaaattc aaaaccggcg ccacctccct cccctgaggt
      841 atttcctgaa acggtgcaat ccgtcatcgt caacaaattt tcgttccgga taccaccacc
      901 tcgggcccca ctgtttccag aaatcctgca attcgagatc gttaaatttc cctgattgag
      961 gattcctcca ccaaaggcgc cggtgctgcc accggaaatc gtcatattgt tcataatgac
     1021 gacagcaccg ttgctaatcg taaaaatgcg aaatgaccca cccgccctga aaacggtaaa
     1081 accaccgccg ttgatggtgg ttgaattccc accattactt gccaaagtcg gaagcgccga
     1141 tgtcagacca atattggccg tgaggttgat gatatccgcc tgactgttgg tgttggcggt
     1201 gttgatggct gtgaccaatt cagccgcagt cgtcactgta aacgttgctg ctggttgaaa
     1261 ttcgccacgc tgggattcca ccggctggtt ttccggttca gttggattga tcacaggcgt
     1321 aacgctgcca tccgcgagca aaatcaaacc ggcagttggg tcatatccat cacttttcag
     1381 ataatgaaac accggtttat agccagttcc atccagcttg attcgaaaga caacgccgtt
     1441 gtcgtcttcg ccaccgttga gcgtcgtgcc atagatattt ccctggcggt cagcaatcag
     1501 gttccccgcc gggcgggcgc cgtccagtcc accaaaattc cagatggtca tcacttcgcc
     1561 ggtttggggg ttgaagccaa aaagagtccc aaggttttgc gctccgccag tttgcgtcgt
     1621 tccaaagagc gtcccgccaa tttccagaag ttgcgcattg acggcctctg tttcagaaaa
     1681 ttcatgccag acagaccgcc ctccgtttgg caatacctga accagtacat gaccatcaac
     1741 acggaaaaaa cttccttccg gcccagggat cacctgaggt tgagattcac ttgattgaat
     1801 tgtggcaggc agaagttccg attgaatctg agactgatgg tgcgctggcc ccgctgactt
     1861 tgaagtcagt gaggtcaaaa gaccaaacga tagcgatgga agggcaagaa agagaaacaa
     1921 tgcaaccaaa cgaaaccggg cagagtggcg tgacgataca ctcatgggac ctccgaaaaa
     1981 ctggtaaata ccagggtggc aaggtgacaa taaacaaggt gacaattgac aaattgacaa
     2041 ggtaacaagg tgcaatagct ttgaattccc cttgttttgt cacatgcgca aaaccgatat
     2101 gatttcactc acccttcatt ttttagattt tttcaggaac cagggttgag gttgtgagta
     2161 agacccaggg taaagcggac aagttcagtt aacctgagta aattttcttt ttccaacagg
     2221 ttacaggcac cagcctgacg tgctttttcc cgaatccgtt cgtcacggta cttggtcacc
     2281 accaccacca cagctttagg gtcaaagtcc agaatctggc gcgtgccggt caatccgtcc
     2341 tgaagc
//
LOCUS       DAOQDO010000937         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41338_length_2346_cov_3.015277, whole genome shotgun sequence.
ACCESSION   DAOQDO010000937 DAOQDO010000000
VERSION     DAOQDO010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41338_length_2346_cov_3.015277"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKZ53_14510"
     CDS             complement(<1..241)
                     /locus_tag="PKZ53_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41703.1"
                     /translation="MDTTQPATDQIPAIDFSPYVPLSLLASGALDPEVARSIRGWPRA
                     RWIITEDAAGLDAATQAELLAILRRGAAWDQRFLLV"
     gene            complement(296..778)
                     /locus_tag="PKZ53_14515"
     CDS             complement(296..778)
                     /locus_tag="PKZ53_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41704.1"
                     /translation="MTTPDVQQYAKTFARRIWHDIHTRIGRLRPAWTGMAEQFADYPA
                     DARPIDLQVDVQASVPAIISTNSVAGTQRHELPLGLLFLVTFRVASQQLILVAVASDE
                     AIAYAPLVGATTNTDQQTRVPLVAFAPETLSEPQALVQPLLRSLLEVVDVGLAELAGV
                     "
     gene            complement(831..>2346)
                     /locus_tag="PKZ53_14520"
     CDS             complement(831..>2346)
                     /locus_tag="PKZ53_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ41705.1"
                     /translation="MGLVQSQGMDRRFFHRMGASLLDRTICATAGSVGYRATNGITMG
                     TDPESFSQAKLILIWGSNTLTSNVHLWPQILKASKNGARVVTIDPYKTRTAAASTEHL
                     AIMPGTDAALALGMMNVIINEGLEDRDYVEKYTLGFEQLKARVQEYPPSKVAEITGLS
                     EETIVGLAREYATTRPAAIRVNYGLQRHYGGGMAMRTITCLPALVGAWRDVGGGILLS
                     TSGTFPLNYPALERPDLIPPGTRLINMSRLGEALTNQNEFGQPAGFDPPVKSVYVYNS
                     NPAAVAPDQGQVFEGLRREDLFTVVHEQFFTDTTDFADIVLPATTQLEHFDIHKAYGH
                     LYLMINEPAIPAYAEARCNSDVFRELATRMEYTDDCLQESDEQLAAQVITSDHPFLEG
                     ITLERLRREGFVRLNIPQPFLPFAEGGFQTPSGKCEFYSESLERQGHDPLPSYTPPRE
                     DRLSNPVLAERFPLALISPPAHNFLNSTFVNQESLKRVEKEPVVELHPIMTVKG"
BASE COUNT          493 a          667 c          691 g          495 t
ORIGIN      
        1 ttacgagcag gaagcgctgg tcccacgcgg cgccgcggcg caggatcgcg agcagctcgg
       61 cctgcgtggc ggcatccagg ccggccgcgt cctcggtgat gatccaccgc gcgcgcggcc
      121 agccgcggat gctgcgggcg acctcggggt caagcgcgcc gcttgcgagc aaggaaagcg
      181 gcacgtaggg cgagaagtcg atcgccggga tctggtcggt tgccggttgc gtcgtgtcca
      241 tgcggctcct cgctttagcc catcaacgtt tcatcgaatc cgaacgttcc cgaacttaca
      301 cgccggccag ctcagccaga ccaacgtcga cgacctccag caacgatcgg agaagcggct
      361 ggacaagcgc ctgtggctcg ctcaacgttt ccggcgcaaa ggcgaccagc ggaacgcgcg
      421 tctgctgatc cgtattggtg gtcgcgccga cgagcggcgc gtaggcgatc gcttcgtcgc
      481 tcgcgacggc gacgaggata agctgctgtg aagcaacacg gaatgtgaca agaaacagca
      541 agcccaacgg cagctcgtgg cgctgtgtgc cggccacgct gttggtgctg atgatcgccg
      601 gcacactcgc ctgcacatcg acctgcaaat cgatcgggcg agcgtcagca ggataatcgg
      661 cgaactgctc cgccatcccg gtccatgcgg gccgcaggcg gccgatccgt gtatggatgt
      721 cgtgccagat gcgtctggcg aaagttttcg cgtactgttg gacgtcaggt gtggtcatag
      781 cacaagcact ttctagcgta acaccgctcc aggagcgccg gtgggggcgc ttagcccttc
      841 accgtcatga tcgggtgcag ttccaccacc ggctcttttt caacccgctt gagcgactcc
      901 tggtttacaa acgtggaatt caaaaaatta tgggctggcg gcgaaatcag cgccagcgga
      961 aaacgttcgg ccaaaaccgg gttgctcaac cggtcttcac gtggcggcgt gtaggacggc
     1021 aacggatcat gtccctggcg ttcaaggctt tcggaataaa attcgcattt tccggaaggt
     1081 gtctggaaac cgccttcggc aaacggcaaa aatggctggg gaatgtttaa ccgcacaaaa
     1141 ccttcgcggc gcaatcgttc cagggtaata ccttccaaaa atggatggtc ggacgtgatg
     1201 acctgtgctg ccagttgttc atcggactcc tggagacagt catcagtgta ttccatccgg
     1261 gtggccagct cgcgaaacac atctgaattg cagcgggctt cggcataagc tggaattgct
     1321 ggttcattga tcatcagata caggtggcca taggctttgt gaatgtcaaa gtgctcaagc
     1381 tgtgtggtgg cgggtagcac aatatcagca aagtcagtgg tgtcagtgaa aaattgctcg
     1441 tgaaccacgg tgaacaaatc ctcacgccgc aagccttcaa atacctgtcc ctggtctggt
     1501 gccacggctg ccgggttcga gttgtagaca taaactgatt tcaccggcgg atcaaacccg
     1561 gctggctgac caaattcatt ctggttggtc agggcttcgc ccaaacggct catattgatc
     1621 aggcgtgttc ctggtggaat caggtcaggc cgttcaagtg ccgggtaatt gagcggaaac
     1681 gtccccgaag tactgagcaa aatcccgccg ccgacatcgc gccaggcacc aaccagtgcc
     1741 gggagacagg taattgtccg catcgccatc ccaccaccgt agtgacgctg gaggccataa
     1801 tttacccgga tggctgctgg tcgcgtggtg gcatactcac gcgctaatcc gacaattgtt
     1861 tcttcagata aaccggtgat ttcagctact tttgaaggtg gatactcttg aacgcgggct
     1921 ttgagttgtt caaacccgag cgtgtatttt tcaacatagt cgcgatcttc cagcccctcg
     1981 ttgatgatga cattcatcat gcccagcgcg agagccgcgt cagttcctgg cataattgcc
     2041 agatgttcgg ttgaggcggc tgccgtgcgg gttttgtaag ggtcaattgt gacaacccgc
     2101 gctccatttt ttgaagcctt cagaatctgc ggccagaggt ggacattcga cgtcagggtg
     2161 ttgctgcccc aaatcagaat caatttggcc tgtgaaaaac tctccgggtc agttcccatg
     2221 gtgatgccgt tggtcgctcg atacccgacg gacccggctg tggcgcaaat cgttcgatcc
     2281 agcagcgatg ctcccatccg atgaaagaac cggcgatcca tcccctggct ctggaccagc
     2341 cccatc
//
LOCUS       DAOQDO010000938         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41508_length_2340_cov_4.126039, whole genome shotgun sequence.
ACCESSION   DAOQDO010000938 DAOQDO010000000
VERSION     DAOQDO010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41508_length_2340_cov_4.126039"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKZ53_14525"
     CDS             complement(<1..316)
                     /locus_tag="PKZ53_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HNJ41706.1"
                     /translation="MPEFAEPIAKLIDEFKRLPGIGHKSAQRLAFHVLRATNDDVERL
                     ANAILDVKQRIMLCSHCHNLTDIDPCRYCSSSQRDQTVVCVVEEAHNLLAIERTGEYH
                     GLY"
     gene            complement(477..830)
                     /locus_tag="PKZ53_14530"
     CDS             complement(477..830)
                     /locus_tag="PKZ53_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNJ41707.1"
                     /translation="MKIPGFDFGQLGGLQNAMQEAQRKQEEMRQTLENERITASVGGG
                     MVMVTMNGLKHLIAVKIDPGLFKEGDSEMLQDLIVAAVHEGERRAEDMAKQQVRNMLG
                     GMDPESLGLKFPGMM"
     gene            complement(962..1948)
                     /locus_tag="PKZ53_14535"
     CDS             complement(962..1948)
                     /locus_tag="PKZ53_14535"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNJ41708.1"
                     /translation="MTNEQNAWIQAAVAERNKKTNGELKVLLENDEVLGIPVIVVSGV
                     RPGKCLLVTAAIHGSEYPGVEALLRLTQAINPLQLRGTLIAVPLVNVPSFYGRGIFVN
                     PADGKNLNRMFPGNPNGSETERIAYALTSELAPLADALIDLHSGDLPEDLTPHLYYRR
                     GGEPDQDQTTRNMAESFGVPFIVEEPLREGNAFGGTWYHSVALQGKPAMLVESGRQGL
                     VEESAIVIHERGVRRVMSVLRMLEETLMPIAFERLSNFFYLNAPSGGLLRMLAKAGDR
                     VAEGQMIASIRNHFGEVVAEVAAPRDAVLLYHISTLSVVNNEPFVEFAVPMS"
     gene            complement(1981..>2340)
                     /locus_tag="PKZ53_14540"
     CDS             complement(1981..>2340)
                     /locus_tag="PKZ53_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNJ41709.1"
                     /translation="GSEMKELDLWEEERLCIQKGIQYISFPIPDFSTPASVREVRLLV
                     GTLTTSLTAGQRIVIHCRGGIGRASLIAACILVWEGKSVDESFAVIQSARGCSVPDTR
                     EQVQWVERFQAFLKAFG"
BASE COUNT          560 a          652 c          531 g          597 t
ORIGIN      
        1 gatacagccc gtgatattcg ccagtgcgtt caattgcgag caggttatgg gcttcttcga
       61 ccacgcacac aaccgtctgg tcacgttgtg aactgctgca ataccgacag gggtcaatat
      121 ccgtcaaatt atgacagtgc gagcacagca taatgcgctg ctttacgtcc agaatggcat
      181 ttgccagccg ctcgacgtca tcatttgtcg cccgaaggac gtgaaaggcc agacgttggg
      241 ctgatttgtg cccgattcct ggcagtcgtt taaattcatc aatcaatttc gcaataggtt
      301 cagcaaattc aggcatgtct gttgttagtg cttggtacct ggtttttggt acctggtttt
      361 tggtacctgg tttttggtac ctggtactcg taacatattg gttccgaaga actaagtact
      421 aagaactaag tactaagcac taagtactaa gcactaagta ctaagcacca gttgggctac
      481 atcattcctg ggaatttgag ccctaatgat tctggatcca ttccgcccag catgttgcga
      541 acctgctgtt tggccatatc ttcggcccga cgttcgcctt catgcaccgc cgccacaatc
      601 agatcctgca gcatttccga atcgccttct ttgaacaggc ctggatcaat cttgaccgca
      661 atcaggtgct tgagcccatt catggtcacc atcaccatcc cgcccccgac tgacgctgta
      721 atgcgttcat tttcaagggt ctggcgcatt tcttcctgct tacgctgggc ttcctgcatg
      781 gcattttgca gaccgccaag ttgtccaaaa tcaaatcctg gaatcttcat accttctcct
      841 cattctggaa ataaaaactc tttgaatcga aaatgaagaa tgaagcattg cgaacagacc
      901 tgacaggttc ttcattcttt attcttcatt cttcattctt cattcttcat tcttcattcg
      961 tttagctcat tggaacggca aattcgacaa acggctcatt gttcaccact gacagagtgc
     1021 tgatgtgata gagcaacacg gcatcacgtg gagccgcaac ttcagccacc acttcgccaa
     1081 aatgattgcg aatcgaagca atcatctggc cttccgcgac ccgatcaccc gctttggcca
     1141 gcattcgcaa taagcctccg gacggcgcgt tgagatagaa gaaatttgac agccgctcaa
     1201 aagcaatcgg catcagggtt tcttcgagca tccgcagcac actcatcacg cgtcggacgc
     1261 cacgttcgtg gataacaatc gcgctttctt caaccagtcc ctggcgacca gactcaacca
     1321 gcatggccgg ttttccctgc agggccacgc tgtgatacca cgttccacca aaagcatttc
     1381 cttcgcgcaa cggctcttcg acaataaatg ggacaccgaa tgattcggcc atattgcggg
     1441 tggtctggtc ctggtcaggt tcgccgcccc ggcgataata gaggtgcggc gtcaggtctt
     1501 cgggcaggtc tccgctatgc aaatcaatca gtgcatcggc cagcggggcc agctctgaag
     1561 ttaaagcgta ggcaatgcgt tcggtttctg atccattggg attcccagga aacatccggt
     1621 tgagattttt cccgtctgcc gggttcacaa aaataccgcg cccataaaag cttgggacgt
     1681 tgacaagtgg cacggcaatc agtgtaccgc gcagttgaag cggattgatc gcctgggtca
     1741 atcgtaacag ggcttcaacc ccaggatatt cactcccgtg gatggctgct gtaaccagca
     1801 aacactttcc gggtcgaaca ccactgacca caatcactgg aattccaagg acttcatcgt
     1861 tttccagcaa taccttgagt tcgccattgg ttttcttatt tcgttcggcc actgctgcct
     1921 gaatccaggc attctgctca ttcgtcatgg tttttaatcc tcatttttca gtgtcagttg
     1981 tcagccaaat gctttcagaa aagcctgaaa ccgctcaacc cactggactt gctcgcgagt
     2041 atcgggaacg gaacagcccc gcgccgattg aataaccgca aagctttcgt caaccgactt
     2101 cccttcccac accagaatac aggccgcaat caacgatgca cgtccaatcc cgccacgaca
     2161 atggatcacg atccgctgcc cggcggttaa actggtggtg agtgtcccga ccagaagccg
     2221 cacctcacga actgaagctg gagttgaaaa atcgggaatc ggaaacgaaa tgtactgtat
     2281 gcccttttgg atgcacaagc gctcttcttc ccaaagatca agttccttca tttcagatcc
//
LOCUS       DAOQDO010000939         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41516_length_2340_cov_2.885339, whole genome shotgun sequence.
ACCESSION   DAOQDO010000939 DAOQDO010000000
VERSION     DAOQDO010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41516_length_2340_cov_2.885339"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..739
                     /locus_tag="PKZ53_14545"
     CDS             374..739
                     /locus_tag="PKZ53_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNJ41710.1"
                     /translation="MNWYLAVLKKYAVFGGRARRMEYWMFAIISTVIYIVLGVVDNVV
                     GTTVAEAGILSSIYSLAVLIPGLAVSVRRLHDTGRSGWWLLVNLTCVGVFVILFFMFQ
                     AGNPGSNQYGPNPKESGGY"
     gene            complement(865..1728)
                     /locus_tag="PKZ53_14550"
     CDS             complement(865..1728)
                     /locus_tag="PKZ53_14550"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ41711.1"
                     /translation="MKSRIWVGFWLIWLGLGEMLIVVHSSTPLPQSVEPSEELNQEAQ
                     VFAQREFETCWTKNGESWFTKEKEGGKYWQVNKIVLKTGSQPISKIDQLNGVSWRGFI
                     DFSSQAQRIYFPDQGWSEWIPGSFASYQLVRKQGRWKIISSAGSAFSKFKHNIGPIEK
                     DIVKPGLNEILQILNEPHLSEEESTKLLADLMFGGSTAPDTRPQIISQSKPGYTEAAR
                     HNKIAGKVVLLVEFRADGTIGEVQVVRSLGYGLDECAVEAARHIRFRPAQKGGMPVTF
                     RSRVEYSFHLT"
     gene            complement(1807..2232)
                     /locus_tag="PKZ53_14555"
     CDS             complement(1807..2232)
                     /locus_tag="PKZ53_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004481200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HNJ41712.1"
                     /translation="MPQACYRELRAYKYQLVEDYEIQINIIPPRDLIFKFVVLTSQGK
                     LWIKKDYAWDGPSGPTIDTKNFMRGSLVHDALYQLMRLGELDYKVYRQRADELLRDIC
                     REDGMSSFRSWYVYQGVRLFGESSARPVPEPEVTIICVP"
BASE COUNT          627 a          580 c          501 g          632 t
ORIGIN      
        1 gtactgacgc attgattctt ctgatttgtc tccaacacaa aatccggtgg aagcaccgac
       61 gtcacagccg ggtgttgagg tcatttgcaa cctggaaaaa acaaatgact tgccaataaa
      121 ccccaagcag cgtatatact cacctcaaag atcactcagc cactattcat ctggtggccc
      181 ttccaaaaaa gctcctcaaa aaaaataaaa tcaattcgaa actggaaata ccttcactct
      241 caggtcaacc gacatttttc aggcatagcc tgtctgaata ctcgtgtggc tatcaattca
      301 gcccttaggc gtcggcaaca ccccagcagt cgcctatcga attctatcgg ggcaacaagt
      361 gaaggagaat gacatgaact ggtatcttgc agtgttaaaa aaatatgccg tgtttggtgg
      421 aagagcacgg cggatggaat actggatgtt cgcaatcatc agcaccgtca tttatattgt
      481 acttggggtt gttgataatg tcgtcggcac aacggttgca gaagcaggta tcctcagtag
      541 tatttactca ctggcggtcc tgatcccagg cctcgccgta tcagtccgtc ggcttcatga
      601 cactggtcgg agcggctggt ggctactcgt taatcttacc tgtgtcggcg tgtttgtcat
      661 tctgtttttt atgtttcagg ctggcaaccc agggtcaaac cagtatggcc ccaatcccaa
      721 agaatcggga gggtattaac ctgataatgc gatcttggat agaagaggtt tgaactctcc
      781 atagaaaatc aaaaaagaga aacagccgag aaacaggggg tggggccgct ttcgagacgg
      841 cacccacccc ttcaacatat gggttcaggt caaatgaaac gaatactcca cccgtgatcg
      901 aaaagtgaca ggcataccgc ctttttgtgc tggccgaaat cgaatatgcc gggcggcctc
      961 aaccgcacat tcgtccaggc catagccaag tgatcggaca acctggactt caccaattgt
     1021 tccatcagca cgaaattcaa ccaataacac cacttttcca gcaattttat tgtgccgggc
     1081 agcttcggtg taccctggct ttgattgaga gataatttga ggacgtgtat caggggctgt
     1141 gctgccgcca aacatcagat ctgcaagcaa ttttgtggat tcttcttcgc tcaggtgtgg
     1201 ttcgttcaga atctggagta tttcattcaa gcctggtttg acgatatctt tttctattgg
     1261 gccgatgttg tgtttgaatt tggaaaacgc tgaaccagca gatgagatta ttttccatcg
     1321 cccctgcttc cttacaagct ggtacgaagc aaagcttcca ggaatccact cggaccagcc
     1381 ctgatcagga aaataaatcc tctgagcctg tgaagaaaaa tcaataaatc ctctccagga
     1441 aaccccgttc aactggtcaa ttttggatat tggctgagaa cctgttttca gtactatttt
     1501 attgacttgc cagtacttcc cgccttcttt ttcttttgtg aaccagcttt ctccattttt
     1561 tgtccagcag gtttcaaatt cccgttgagc aaacacctga gcttcctgat tgagttcctc
     1621 agaaggttca actgactgcg gcagaggtgt tgaagagtga accacgatga gcatttcccc
     1681 taaacctaac caaatcaacc aaaatcctac ccaaatccgt gatttcatac ccaatcctcc
     1741 ttttgaacaa cctgactgga cgggttcaaa tttccacctg atccatttct gagagggtta
     1801 atcttgttat ggaacacaaa tgatcgtgac ttcaggttct ggaacaggcc gggcactact
     1861 ttcgccaaac aatcgcaccc cttgatagac gtaccacgac cgaaatgacg acatcccgtc
     1921 ttcgcggcaa atatcccgca gcagttcatc cgcccgctgg cggtatactt tataatccag
     1981 ttctccaagt cgcataagct ggtacaaggc atcgtgaacc agcgacccgc gcataaagtt
     2041 tttggtgtca atcgtcggcc cactcggccc atcccaggca tagtcttttt tgatccataa
     2101 ctttccctga ctggtcaaaa caacgaattt aaatatcaga tcccgtggtg gaataatgtt
     2161 aatttgaatc tcgtagtcct caaccaactg atatttgtag gctctcagtt ctcgatagca
     2221 ggcttgcggc atagtaaatt cacctcaaat tattcatact acaggtgaaa gtaatttttc
     2281 ttcattctag tgatcaacat tgtatcggaa agtcaatgtc ggcgtaatag ctatcagtat
//
LOCUS       DAOQDO010000940         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41551_length_2339_cov_3.072680, whole genome shotgun sequence.
ACCESSION   DAOQDO010000940 DAOQDO010000000
VERSION     DAOQDO010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41551_length_2339_cov_3.072680"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKZ53_14560"
     CDS             <1..435
                     /locus_tag="PKZ53_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41713.1"
                     /translation="PGTRNPEPGTRNPEPGTQNPEPRTLSTMHLYQRQQFDFLLMTSA
                     ERFVERITQRNEGAANALRKLQADANGEGIWLDEFVAVLFEDFLLNNTAGACFVLEAL
                     EKRTVPDIAGGKIELVLQLLARRAFADLLKQKVEEVLSRAWG"
     gene            578..1477
                     /locus_tag="PKZ53_14565"
     CDS             578..1477
                     /locus_tag="PKZ53_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNJ41714.1"
                     /translation="MGISLPVLKAVPPPPPEPVEPKALTFEDLAAAVDQAVSQVQMFE
                     SPELGKAMRMKDAIEAFHKYGLTKIVQKLKSDPHGKELLFDLVDDPGVYALFVLHGLV
                     KPSLATRVVQVLETIKPYMQSHGGDVELDRVEDRTVFVKLHGACLGCSHSAVTLREGV
                     EEALKTRIPEITSIEVVPNDPGPTLIHLGSGSTTNQHGWIEGPRVEDVPVNSKQVLQA
                     GDTPILLVNVDNRLSAFHNVCPHQGLPLDGGMFDPEGLELTCPWHGFRFHAATGECLT
                     VPQVQLESFPLRVENGRIFVRPN"
     gene            1477..>2339
                     /locus_tag="PKZ53_14570"
     CDS             1477..>2339
                     /locus_tag="PKZ53_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41715.1"
                     /translation="MEPLIWLGAYSPGKLALPAAQPTPSQVYAPRGVFLGDDLLVVAD
                     SGNHRVLIWHSIPVKDGQPADVVLGQPDFYSEGPNAAGQGTGNGLHLPTGVGVYENRL
                     FVADAWNHRILVWNTIPETSNQPPDWVLGQHSLAESEVNRGQSVTDSTLYWPYSLAYF
                     GGWFYVTDTGNRRVLGWRGLPETDRPADLVLGQPDLSSNLENRGKAIGPDSFRWPHDL
                     AGDDAVMFVADAGNHRVLGWSPHPEGDAPAQVCFGQPTFEQAQELPYVAQGPQKLRFP
                     YSIAFENGRLAV"
BASE COUNT          533 a          640 c          635 g          531 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac ccagaaccca
       61 gaacccagaa ccctcagcac tatgcacctt taccaacgac aacaatttga cttcctgttg
      121 atgacctcag ccgagcggtt tgtcgagcgc atcacccagc gcaacgaagg tgcggccaac
      181 gcgctccgca agctgcaggc agatgccaat ggcgaaggaa tctggcttga tgaatttgtc
      241 gccgtactat ttgaagattt tcttttgaac aacactgccg gcgcctgttt tgtgctggag
      301 gcgcttgaaa aacgcacggt tcctgacatc gctggcggga aaatcgaact ggtgctgcaa
      361 ctcctggctc gtcgggcatt tgcggatttg ctcaaacaaa aagttgaaga agtccttagt
      421 agggcttggg gctaaagact ttgggctcag ggctgagggt tgaagactcg caagcctggg
      481 gctgaagaac tcgggttcaa caccctgagc ccgttttctt cagcccgaca tcttcagccc
      541 gttttcttta gcccctgtat tcggaaagga accacctatg ggcatttcat tacctgtctt
      601 aaaagccgta ccaccgccgc cacctgaacc ggtcgaaccc aaagcactga cgtttgaaga
      661 cctggcggcg gcggttgatc aggcggtgag tcaggttcaa atgtttgagt cacccgagct
      721 gggcaaagcc atgcggatga aagacgccat tgaggcattt cacaagtatg ggttaaccaa
      781 aattgtccaa aagctcaaat ccgacccgca cggcaaagaa ctcctgtttg atctggtgga
      841 tgatccaggc gtgtatgccc tgtttgtgct ccacggactg gtcaaaccga gtctcgcgac
      901 acgggtggtt caggtactcg aaaccatcaa gccctatatg caatctcatg gcggagacgt
      961 cgaattggac cgcgttgaag atcgaaccgt ttttgtcaaa ctccacggcg cctgtttggg
     1021 gtgttcgcat tctgccgtca ccttgcgcga aggcgtcgaa gaagccttga aaacccgaat
     1081 tcctgaaatc accagcatcg aagtcgttcc aaatgatccc ggaccgacgc tgattcatct
     1141 tgggagtgga tccacaacca atcaacacgg ctggattgaa ggcccacgcg tggaagatgt
     1201 tcctgtcaat tcaaaacagg ttctgcaggc tggtgatacc ccgattctcc tggtcaatgt
     1261 agataaccgg ctgagcgcct ttcacaacgt ctgtccccat caaggcctcc ctttggatgg
     1321 cggaatgttt gacccagaag gactggaact cacctgcccg tggcatggct ttcggtttca
     1381 cgctgccaca ggcgaatgtc tgaccgtgcc acaggttcag cttgaaagct ttccgctccg
     1441 ggttgaaaac gggcgcattt ttgtgaggcc aaactaatgg aaccactgat ctggctgggc
     1501 gcctactcgc cggggaaact ggcgcttccg gcagcccaac ccacgccgtc acaggtctat
     1561 gccccacgag gtgtttttct tggtgatgat ctgctggtgg tggctgattc tggaaatcat
     1621 cgggtgctga tctggcattc aattccagtt aaagatggcc agccggcaga tgttgttttg
     1681 ggccagcctg atttctactc tgaaggccca aacgccgctg gtcaaggtac tggaaatgga
     1741 ctgcatcttc cgaccggcgt tggggtgtat gaaaaccggt tgtttgtagc cgatgcctgg
     1801 aatcatcgga ttctggtctg gaacacaatc ccagaaacgt caaaccagcc gccggattgg
     1861 gttttgggcc agcattcact ggcggaatca gaagttaatc gcgggcaatc ggtcactgat
     1921 tcaaccctct actggcccta ttcgctggcg tacttcggcg gctggtttta cgtgaccgac
     1981 actggaaacc gtagagtcct cggctggcgc gggctccctg aaacagatcg tccagcggac
     2041 ctggtgcttg gacaacctga tctgtcgtca aatcttgaaa atcgtggaaa agccattggc
     2101 cctgatagtt ttcgatggcc gcacgatctg gccggagacg acgctgtgat gtttgtggct
     2161 gatgcgggta accatcgcgt gcttggctgg tcaccacatc cagaaggtga tgctccagct
     2221 caggtctgct ttggacaacc aacctttgaa caggcccagg aacttcccta cgtggcacaa
     2281 gggccgcaaa aactgcgatt tccgtactcc atcgcgtttg aaaacggacg actggcggt
//
LOCUS       DAOQDO010000941         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41555_length_2339_cov_2.901051, whole genome shotgun sequence.
ACCESSION   DAOQDO010000941 DAOQDO010000000
VERSION     DAOQDO010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41555_length_2339_cov_2.901051"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..119
                     /locus_tag="PKZ53_14575"
     CDS             12..119
                     /locus_tag="PKZ53_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41716.1"
                     /translation="MLVTRRGMRLSVQPVTKEEWQVIESLVPVAPETAG"
     gene            349..>2339
                     /locus_tag="PKZ53_14580"
     CDS             349..>2339
                     /locus_tag="PKZ53_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ41717.1"
                     /translation="MVLVVDDDSYTRRIIRLLLEKEKYQVQEAEDGETALRMIQTTRP
                     DLILTDLMMPGINGIETTRQVFQLPGCRRIPIMMITAVNEPDIVDQAFAVGVADYFVK
                     PINWPVLRQRIKRLLTPGSVEFRGEVPATFSQTLIDQALDGVITLTESGIIQFANAAV
                     MTMFGYDPGSLEGLPIYILFPNFRIPHSKTEGILLEMQGHHRELRPFPTEVSVNLLKL
                     GKERFLSLVIRDLRRRASALQKQAELTTNLDGISSLKGRAIEAAQVGFVIVDLTLPDA
                     PLVFANQAFCELTGYPLGEVLNRNCRFLQGPDTDPETVEQLRAAISRKEACTVVIKNY
                     RRDKTPFWNRLSIAPIATTTATITKFVGIQTDVTLQKQITEASNPADAFLLMIIRTIP
                     SGIAAVDASGKLTFVNQRLCEITGYSAEDLVGTSLSRLLLLPGQIGALRRKSQSGVTE
                     LKLAEQELNLRRADQQIIFVRLRLATLPSAGSTVVVVEDLSERERTQALIKQAEMEWV
                     ATVDTVSDLILLEDDSHRLRRCNYAAASFFYRHVQDMVGENLFELFSALQIELHPEVP
                     LNTWHPLRLPIAEVQFTNREGWFEITNYQISAERGFGLVWVHIVKNITSRKQVEADLL
                     RLNAAIEQAADAIVLTDTAGTIVYANPAFEKTTGYRRADA"
BASE COUNT          561 a          627 c          594 g          557 t
ORIGIN      
        1 ggctcgaaaa tatgcttgtc acccggaggg ggatgcgact ctcagtccaa cccgtaacaa
       61 aagaagagtg gcaggtgatt gaatcactgg tgccagttgc cccagagacc gcaggctaaa
      121 aagctggctg ggactgaagg ggcgcggaaa tcatcagaaa atttggcaaa aagcgatagc
      181 cctcgccatc ctggcagatt atactgtcag agactgcttt cataaacctc ctgcaaaatc
      241 cctcttgcat tccacagcca gtctggtttc aggcaacttc cccccgcttg ccctgtcccc
      301 cgccaagcta tgctccgtac ccaaaaatta agttcccgga aacagtttat ggtgttggtg
      361 gtcgatgatg attcctacac ccgccggatc attcgcctcc tgcttgaaaa agaaaagtat
      421 caagttcagg aagccgagga cggtgaaact gccctgcgga tgatacaaac aacccggcct
      481 gatctgattc tgaccgatct tatgatgcct ggaatcaatg gtattgaaac cactcgacag
      541 gtttttcaac ttccaggttg ccgccgaatt ccgatcatga tgatcacggc tgtgaatgag
      601 cccgacattg tggaccaggc ctttgcggtt ggggtggccg attactttgt caagccaatc
      661 aactggccgg tgctgcgcca acgaatcaaa cggctcctca cgcctggcag cgttgaattt
      721 cgaggcgagg ttccggcaac gttcagccag acactgattg accaggcgct cgatggtgtg
      781 atcaccctca ccgagagcgg cattattcaa tttgccaatg cagcagtcat gaccatgttt
      841 gggtatgatc cgggcagtct ggaagggcta ccaatttata tcctctttcc aaatttcaga
      901 atcccccatt ccaaaacgga agggattctg ctcgaaatgc agggacatca ccgcgaattg
      961 cgacctttcc cgactgaggt gtccgtaaat ttgctcaagc ttggcaaaga gcgttttctc
     1021 tcgctggtga ttcgagacct gcgccgacga gccagtgccc tccaaaaaca agctgaattg
     1081 accaccaacc tggatggtat ttcctctctc aaagggcgcg cgattgaagc cgctcaggtg
     1141 ggatttgtca tcgttgacct cacgttaccg gatgctcccc tggtttttgc caaccaggca
     1201 ttttgtgaat tgactggcta cccgttaggt gaagtcctca atcgaaattg ccggtttttg
     1261 cagggcccgg atacggatcc agagaccgtt gagcagctcc gggctgccat ttccagaaaa
     1321 gaagcctgta cggtcgtaat caagaactat cggcgggata aaaccccgtt ttggaatcgc
     1381 ctgtcaattg cccccattgc cacgacaacc gcaacgatta ccaaatttgt gggaatccag
     1441 acggacgtca cccttcagaa acaaatcact gaggcctcca atccagccga tgcctttctc
     1501 ctcatgatta ttcgaacgat tcccagtgga attgccgcgg ttgacgccag cgggaagtta
     1561 acctttgtca atcaacggct ctgtgaaatt actggatatt cagccgagga tttggtgggc
     1621 acctcattgt ccaggctgtt gctcctgcct ggtcagattg gggccctgcg ccgcaaaagc
     1681 cagtcagggg tgactgaact gaaactggct gagcaggaac tcaacctccg acgggctgac
     1741 caacagatca tttttgtccg gctccggctg gcgaccttac ccagtgcggg cagtaccgtg
     1801 gtggtggtcg aagatctcag tgaacgagaa cgcacccagg cactgatcaa acaggctgaa
     1861 atggaatggg tggcgacggt tgatacggtt tctgacttga ttttgctcga agacgattcc
     1921 caccgcctcc gccgatgtaa ttacgcggct gccagttttt tctatcggca cgttcaggac
     1981 atggttgggg aaaatctgtt tgagctattt tctgctctcc agattgaact ccaccccgag
     2041 gtcccactca atacctggca tccactccgc cttccaatcg ctgaagttca atttaccaac
     2101 cgcgaaggat ggtttgagat taccaattat caaatttcgg ctgaacgagg ttttgggttg
     2161 gtttgggtcc acatcgtcaa aaacattacg tctcggaaac aggttgaggc tgacttgctg
     2221 cgtctcaatg ccgccattga acaggcggca gatgcgattg tgttaaccga tactgccgga
     2281 acgattgtgt atgccaatcc ggcctttgaa aaaacgaccg ggtatcgtcg agccgacgc
//
LOCUS       DAOQDO010000942         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41582_length_2338_cov_3.363995, whole genome shotgun sequence.
ACCESSION   DAOQDO010000942 DAOQDO010000000
VERSION     DAOQDO010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41582_length_2338_cov_3.363995"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(990..>2338)
                     /locus_tag="PKZ53_14585"
     CDS             complement(990..>2338)
                     /locus_tag="PKZ53_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ41718.1"
                     /translation="GVQDGRIFRDALPSTCPSKAYPGIFGVGTNFNYDAYTFVNNMAS
                     AQCITVNFDPDTVSGACGTNAHASAYLTSYNPASQATNFVGDVGSSAAQPFSFTVPAN
                     GTFLVVVTNTTAQATCSYQFTVVGDVSCGGGSAATATWSVLMPAGTTAGKVKNPKGVT
                     QTSDGVNIYVADTGNNRIQKSTDSGATWAVMPNTTAAPLGFKSPAAVAVETDDPNVVY
                     VADTGNNRVLASSDGGATWTILAGTGSNPNQVRGPEGLVVDCDGNLWIADTLNNRIVA
                     YAALVNPPRGTRVAVTPGTGVVVAGLGSALNQVRAPKAVTVDVLTGDLYVADTGNNRV
                     THYTNGPVLTPVALVDASLVASSGSALGQVRSPAGITVSGGMAPEMVRNGLTKVANTG
                     VVSLVIGDTLNNRIQYAEISGTAQVGAFALLKGTPAGQTATIGSAVGQFRAPTGVR"
BASE COUNT          541 a          583 c          656 g          558 t
ORIGIN      
        1 gtacagatgg tcggttgatc atttatcccc ctaaattgtt cttttaattc ctgaatcctg
       61 atttttcgtg cgggaagtgg aatcaagatt ggataatttg gaacgggcaa cttccagaga
      121 aacatcagtt cgcatcagtt ttgtggtgtt tgaactgaac acattcctga tgatggcact
      181 gatcgaagta ccgattggtt gaaagagaca ggttgtattg gttgtataac ctattgaaga
      241 gtaaaggagc taaggcaact gaaatatttt tgtaacataa gtgaaggctg agggtaaatg
      301 cctttcactt cggtttgaag aaagccaggg cctgggggct gtagacattg gggcagaaga
      361 caaccggctt ggagcaatca aaaagatgag ggatgacgaa aaccaggtct tgatcaccac
      421 ttcttcagcc tgaagttttc agtccagaaa ctgggggcac ccggcacctg cttcgcaggt
      481 taaaccgaaa tctcgctgtc attggcggat ggggataaac acaaaaaaat aaccctgtgc
      541 ttctataagc ccagggtttt caacggttag agtaatgata aacccaggtg gacaggttcc
      601 aaccagcatt gaaagcaatg gtgagagctt tcatttcctt ggtagaacaa ggccaaaaag
      661 agtatggcgg aatggatttg caactggcat ccgtagtcct actcattttt atgagtagga
      721 ctactcattt tttggctgaa gatcaaagaa gattgggcga acgaaagtgg gctccggatt
      781 ttgggctgga acctttgggt taaaaacttc agcttcaaat tcaaaacaaa aaggggaggc
      841 tttgaaattt tcaaagcctc tcctttttgt tttacttggg cttcagccct gagcttgcga
      901 gtcttcaacc ccaagcccca agcccaaggt cttcagcctg tcttcttcag ccccaggccc
      961 tgagccctga gagccctgag cccgaaaaac taccggactc cggttggagc acggaattga
     1021 ccgaccgccg agccgatagt ggcagtttgg ccagccggtg ttcctttcag gagcgcgaac
     1081 gccccaacct gcgccgttcc actgatttcc gcatactgga tgcggttgtt gagtgtgtca
     1141 ccaatcacca agctgacgac gcccgtgttg gcgactttgg tgaggccatt gcgcaccatt
     1201 tcaggtgcca tgccgccgga aacagtgatg ccggctgggc tgcggacctg accaagcgct
     1261 gagccgcttg atgcaactag tgatgcatca accagggcga ccggtgtcaa aaccggaccg
     1321 ttggtgtaat gcgtgacgcg gttgttgcca gtgtctgcca catagaggtc gccggtcaag
     1381 acatcaaccg tgaccgcctt tggtgctcga acctgattca aggctgaacc aagaccggca
     1441 accaccaccc ctgttccagg tgtgacagcg actcgagtac ccctcggtgg gtttacgagt
     1501 gccgcatagg ccacaatgcg gttgttgagc gtgtcggcaa tccagaggtt gccgtcacag
     1561 tcaacgacca gaccttctgg tccgcgaact tgatttggat ttgaaccagt tcctgccaga
     1621 atggtccatg tggcgccgcc atctgacgat gccagcacgc ggttgttgcc cgtgtcggcg
     1681 acatagacca catttggatc atcagtctca acggcgactg ccgctggcga cttaaatcca
     1741 agtggggcgg cggtggtgtt tggcattaca gcccaggtgg cgccgctgtc ggttgatttc
     1801 tggatgcggt tgttgcctgt gtcggccaca tagatgttga cgccgtcgct ggtttgggtc
     1861 acgcctttcg ggtttttgac tttgcccgcc gtcgtgccgg ctggcatcaa cactgaccag
     1921 gttgctgttg ccgcgctgcc accaccgcag gagacatcac ccaccaccgt aaactgatag
     1981 ctgcaggttg cctgagcggt ggtgttggtc accaccacca ggaaggtgcc gttggcgggt
     2041 acggtgaatg agaacggctg ggcagcactc gatccaacat ccccgacaaa gttggtcgcc
     2101 tggctcgccg gattgtagct ggtgaggtag gcgctggcgt gggcattggt tccacaagcc
     2161 ccactgacgg tatctggatc aaagttgacc gtgatgcact gtgccgatgc catgttattg
     2221 acaaacgtgt aggcatcgta gttgaagttt gtcccgacac cgaaaatacc tgggtaggct
     2281 ttcgacgggc aggtacttgg aagtgcatcg cggaaaatac gtccatcctg gacgccag
//
LOCUS       DAOQDO010000943         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41583_length_2338_cov_3.309680, whole genome shotgun sequence.
ACCESSION   DAOQDO010000943 DAOQDO010000000
VERSION     DAOQDO010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41583_length_2338_cov_3.309680"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKZ53_14590"
     CDS             <1..676
                     /locus_tag="PKZ53_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ41719.1"
                     /translation="LTIPQGKTLAIVGHTGSGKSTLINLIPRLLDAAPGEVLIDGRPI
                     REIPLQVLRQNIGYVPQETFLFGDTVAANIAFGVPGASREAIEQVAEQSALRADIATF
                     PKGFETIVGERGITLSGGQKQRTAIARALLRNPKILILDDSLSAVDTYTEEKILGHLR
                     EIMQDRTTILVSHRISTVKEADHIIVLHDGRIAEQGTHDWLLKQNGPYAALYEKQLLE
                     EELATL"
     gene            673..1311
                     /locus_tag="PKZ53_14595"
     CDS             673..1311
                     /locus_tag="PKZ53_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ41720.1"
                     /translation="MTPNEQSRLQPSTEFSGDQLLGLLIEAAGKGNLSLVTDLLAKGA
                     AVNGGFSDGYTPLMAAAHSGQAQVVTTLLHHGAAPDQGERQGMTALMFAAEDGSVEAV
                     RALVEGGATINYQVQDEFSPFSGWTALLFATDNGHLPTATYLISQGANVDLCDSEGET
                     ALMKAAALGHVALVECLVKAGSTVSLANENGDTALTLAEEMGHRKIIGLLKH"
     gene            2030..>2338
                     /locus_tag="PKZ53_14600"
     CDS             2030..>2338
                     /locus_tag="PKZ53_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41721.1"
                     /translation="MFSRTNRSLVKKRPFLTFAVALLGFSLVNILPFTATSGTQASTV
                     VTPTPFEMAFQGTNTPPTFTPGPPLTVRQGERKAVIIGSVNDAEQQPQALGVTVVRLL
                     S"
BASE COUNT          575 a          625 c          574 g          564 t
ORIGIN      
        1 actgacgatt ccacaaggga agacactggc gattgtgggt cacacggggt ctggaaaatc
       61 aacgctcatt aacctgatcc cacggcttct ggatgcggcc ccgggagagg tgttgattga
      121 tggccgacca attcgggaaa tcccgctcca ggtcctgcgc caaaacattg ggtatgtccc
      181 tcaggaaaca ttcctgtttg gcgatacagt ggctgccaat atcgcctttg gggtgcctgg
      241 ggcatcacgc gaagccattg aacaggtagc cgaacagtcc gcccttcggg ctgacatcgc
      301 cacgtttccc aaaggatttg aaacaattgt cggcgaacgt ggcatcacgc tgtcgggagg
      361 tcaaaaacag cgaacggcca ttgcccgtgc cctgctgcgg aaccccaaaa ttctcattct
      421 ggatgactct ctctcagccg ttgataccta tacagaagag aaaattcttg ggcatttgcg
      481 cgagattatg caggatcgca ccacaatcct ggtttcacac cgaatttcca cggtgaaaga
      541 agccgaccac atcattgtcc tgcacgatgg ccggattgcc gaacaaggca cccatgactg
      601 gcttttgaag caaaacggtc cgtatgccgc actctatgaa aaacagctcc tggaggaaga
      661 actcgcaact ttatgacacc aaacgaacaa tcccggcttc aaccctccac cgaattttcc
      721 ggtgaccagt tacttggcct cttaattgaa gccgctggaa aaggcaatct gtctctggtg
      781 actgacctgc ttgccaaagg tgcggcagtc aatggcgggt tcagtgatgg gtatacgccc
      841 ctgatggccg ccgcacactc tggccaggca caggttgtca ccacgttact ccaccatggg
      901 gcggcacctg atcagggcga gcgccagggg atgacggcac tgatgtttgc ggctgaagat
      961 gggtcggtgg aggcagttcg ggccctggtt gagggcggag cgaccatcaa ttatcaggtt
     1021 caggatgaat tttcgccatt ttccggctgg acagcactcc tgtttgccac tgataatggc
     1081 catctgccaa ccgctaccta tttgatctca cagggtgcca acgttgatct gtgcgatagc
     1141 gaaggtgaaa cggcgttgat gaaggcggct gcccttggtc acgtcgcgct ggtcgaatgc
     1201 ctggtgaaag ctgggagtac ggtcagcctc gccaatgaaa acggagatac ggcgctcact
     1261 ctggccgagg agatgggtca tcgaaaaatc atcggtcttt tgaaacacta acctccacca
     1321 tttcaggtca accagattca gaccttcaaa agggttcaga atggaaccga actccctgct
     1381 tgagcgagtc tatgatggat cggcagaact caaagcctcc gtcggtgatt ttgccttcaa
     1441 gaaatggaaa aaccctggtg ccggtacctg agtaattggt tctcattttt tccagaaacc
     1501 gtttggaaat ggtttctttt ttttcagtca accagcgttt ttttcacaag aaaaatcctg
     1561 ggccttgtgg tttaatacct aacgactctc tgccaatcaa cattcgtttt ttgcatccat
     1621 ctgtgccacc gaacgggcaa atgtccggat tgacaagtac ttcaatccga acatagttcc
     1681 ccctcaccac tcaactcagt ggcacttttc atttcagagc tgaaagaaac tcattcgtga
     1741 cactggcccg aaggtcgtga actggctttc gtgcacggta tttgcagcac ctgatttaac
     1801 cacttttacc gggaattttt tcccattaaa cagaggccgg ttagggttga caaacactca
     1861 gagatgttca ttatcgagga acgcaaaaat tccctcgatg atacatctcg tcccaatttc
     1921 tcaagttttt atccgagaaa tccaacattc gacttattca ctgattctcg aacatagatc
     1981 cgtgtttgaa tcggcctatt acaagaaccc cagaaaggag aacaaactca tgttttctcg
     2041 caccaatcgg agtttggtaa agaagcggcc gttcctgacg tttgcggtcg cccttctcgg
     2101 attttcgctg gttaacatac tcccatttac tgcgacctca ggcacacagg cttcaactgt
     2161 ggtgacacca actccttttg agatggcgtt tcaaggcacc aatacccctc cgaccttcac
     2221 accaggcccg ccgctgacag ttcgtcaggg tgagcgtaaa gcggtgatta tcgggagcgt
     2281 caacgatgcc gaacaacaac cacaagcctt gggcgtaacc gtcgttagat tactctct
//
LOCUS       DAOQDO010000944         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41588_length_2338_cov_2.819536, whole genome shotgun sequence.
ACCESSION   DAOQDO010000944 DAOQDO010000000
VERSION     DAOQDO010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41588_length_2338_cov_2.819536"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..776)
                     /locus_tag="PKZ53_14605"
     CDS             complement(108..776)
                     /locus_tag="PKZ53_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNJ41722.1"
                     /translation="MPSFLLFRRTLAAFVIGFVLTAGFFPENLFASQRKRAAAKRSAA
                     KRSGGAKKSARASKSSRSRTRASSKKSRGKTSARSSRSRRGKARYTRARRGGRRVVYS
                     RRGRRGRARYSAPVASTPRAPRTPPRAQIPSDRVTEIQDALKKAGFYQGEMTGEYDSA
                     TREAMSAFQRANGIKETGMPTAHALAKLGLTKKTSAAGDTSAPGVKFEPSKDQPQNEE
                     DNNQ"
     gene            1305..>2338
                     /locus_tag="PKZ53_14610"
     CDS             1305..>2338
                     /locus_tag="PKZ53_14610"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41723.1"
                     /translation="MWLSLGVTLCLLSGSVFALDPQKSLTQYGHDVWQDGLPHTSILS
                     LCQTRDGYLWFGTHEGLVRFNGTEFTVFDKRNTPEMKGNSIWSLFEDRSGRLWIGTLS
                     GGLVCYQNRAFRAYSMRDGLAGDFVYAFCEDAAGDIWVGTKSGLSRLVNDRFESFTTY
                     HGLSSNNILSLCADTSGDIWIGTDGGGLNRFHQGQFTKVEGLPHATVSSLWCGRNGRL
                     WVGLYGGGFGYFEQGKFVGYTQRDGLSSNLVWTLLEDPAGTLWVGTEGGGLNRFQNGV
                     LQVYTTRQGLSQDYARSLCVDFEGSLWIGTNAGLNRLKDGKFINYSTQEGLSNNNIRS
                     VLEDRVGRMW"
BASE COUNT          528 a          549 c          665 g          596 t
ORIGIN      
        1 aaagaattgt tcgtaaacag atcaaataga acaaaataca cttgacagat aactcaaaac
       61 cccaacactt gacgactgac gactgacgac tgacgactga tgactgacta ctgattattg
      121 tcttcctcgt tttgcggctg gtctttggat ggttcaaact tgacacctgg tgctgatgtg
      181 tcgccagcgg ctgaagtctt tttcgtcaat cccagtttgg ccagtgcgtg agccgttggc
      241 atcccggttt ccttgattcc gttggcgcgt tggaacgcgc tcatggcttc acgcgtggct
      301 gaatcatatt caccggtcat ttcaccttga tagaacccgg cttttttcaa tgcatcctgg
      361 atttcggtga cccggtctga aggaatttga gcccgaggcg gtgtccgtgg ggcgcgtggt
      421 gtgctggcca ctggggcgct gtagcgggcg cgtccgcgcc gaccccgacg tgagtacacg
      481 acacggcggc ctccgcgacg agcgcgggta taacgagcct ttccacgccg actccgactt
      541 gagcgggcac tggttttccc acggcttttc ttgctgctgg cgcgggtccg cgagcggctg
      601 gatttactgg cacgggctga ttttttggca ccgccagagc gtttggcggc tgatcgttta
      661 gccgctgcgc gtttgcgctg gctggcaaaa aggttttcgg ggaaaaaacc ggcagtgagc
      721 acaaagccga tgacaaaggc ggccagcgtt cgacgaaaca acaaaaaaga aggcatgggt
      781 actgctccac aagtgattgg gtcggcctgg aacggaagga aaacccccag aagtgagggc
      841 caaccgggta aaagggttcg cattatgcca ctcaaggctc aaggctctca aggtggttca
      901 acggtgaaag tgcattcaac tggtattaca ggattgacga cgacgactgg taacgattga
      961 atcaaaaccg ccagacccgt atgccgcctg atccagatgc gaaggaaaaa tcatgctgct
     1021 tcacgtggta aagagggatt ttatggtata gttgggccgg attttttgaa attccagctt
     1081 cttgggctca gaaaatcagg accttgacgt tgttttcttt tttcctcaaa aatgcagaac
     1141 cgctgctgtt tgtcatcaag gcattcccgg gaagcagata aaaagtccgt ccgccgccaa
     1201 agttttgatc actgattggt tgtgtgtctc ttcagatgtc tcctgatcca aaatgtttga
     1261 cccgatctgg gcagaaaaaa tttcacattt ggacagtgtt ggggatgtgg ctatccctgg
     1321 gtgtcacgct gtgtctgctt tctggttccg tttttgccct cgatccacaa aaaagcctga
     1381 ctcagtatgg gcacgatgtc tggcaggatg gattgccgca tacatcaatt ctttccctgt
     1441 gtcagaccag ggatggatat ttatggtttg gaacccacga aggtctggtt cgatttaatg
     1501 gcacagagtt tacggtcttt gacaagcgca ataccccgga aatgaaaggc aacagtatct
     1561 ggtcactgtt tgaggaccgg tcaggtcggt tgtggattgg tactctcagc ggcggtctgg
     1621 tgtgttacca gaatcgtgct tttcgagcct attcaatgcg tgatggactg gctggcgact
     1681 ttgtgtatgc cttttgtgaa gatgccgccg gagatatctg ggttggcacc aaatcgggac
     1741 tcagccggct ggtcaacgac cggtttgaat catttaccac atatcatggc ctttcgagca
     1801 ataatattct ctctctgtgt gccgatacga gcggagatat ctggatcggc accgatggtg
     1861 gcgggctgaa ccgatttcat caaggccagt ttacaaaagt cgaagggctg ccgcacgcca
     1921 cggtctcatc gctctggtgc gggcgaaacg gacggctgtg ggttggtttg tatggtggtg
     1981 gatttgggta ttttgagcag ggaaaatttg ttgggtacac ccagcgcgat ggcttatcga
     2041 gcaatctggt ctggaccttg cttgaagatc cagccgggac gctctgggtt ggtactgaag
     2101 gcggcggcct gaatcggttt caaaatggag tccttcaggt atacaccaca cgccagggac
     2161 tctcacagga ttatgcccga tcactgtgcg tggactttga ggggagcctc tggattggca
     2221 ccaatgccgg gttgaatcgc ctaaaagatg gaaaattcat caattattca actcaggaag
     2281 gattgtcaaa caacaatatc cgctcagttt tagaagatcg tgtcggacgg atgtggat
//
LOCUS       DAOQDO010000945         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41594_length_2338_cov_2.547087, whole genome shotgun sequence.
ACCESSION   DAOQDO010000945 DAOQDO010000000
VERSION     DAOQDO010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41594_length_2338_cov_2.547087"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2338)
                     /locus_tag="PKZ53_14615"
     CDS             complement(<1..>2338)
                     /locus_tag="PKZ53_14615"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ41724.1"
                     /translation="AEVDPQTGRVRGVRQGFATVTASNGNITDSVFVVVTRVKNGPAE
                     KVPGETKVDQNGRLYLTNPAQNTILRATDALKASLKIFAGQRGARGLQDGSVTQSLFA
                     GPTAIGIDGRVQGGVYVADTLNHCVRKIGFTNQVETVLGTGDPGTPVFDSQGSAAFNQ
                     MVFRSPRGIAVESSGNLFLADTDNHAIYFVNLSAKRVWLLAGNPGQSGVLDGTGREAR
                     FSRPAGLALNSTGRVLAVADQDNNRVRLLELTQTESGGLTCRVSTVGTQSAARAFEIT
                     AQSDQPIEFSKPISLSFDSVDNLNVIDETGAFLITQPLGDFARKLDLAQPEVTFHQPV
                     SVTIRGTDTFVLDPGATEDRQALKIVTVGGPEITNVNRETVRAEGGEEVIVTGKNFAP
                     ESIVSLGDALVENLQVESATECRFRVPRQRGYGNRTLTIQTRGGLVQRSLAVIPKPLS
                     ELTAGEITTVVGGMQGVGDGRMATAATVNYPTSTAIDSSGNVYVADASHRIRRIDAQT
                     GIITTVAGTGRGGFSPDGTLAVTADLAADKVVIDQAGNLLINDNFRIRRIDLRTGVLT
                     TIAGTGSPSFRGDGGPAIEAGINPDSFAIDRTGNLFIVDRSSRIRRVDAQTGIITTVA
                     GTGKREYSGDGGPATHAGLFRPSNVVVDSQGTLFIADRQNHRIRRVDAQTSIITTVAG
                     SGAEGLSGDGGPATAARLSYPLDVVIDADDNLLITDAGNQRIRKVNQSSGGISTIVGN
                     GPAGFGGFGFGGDNGLATQAQLGYPHGIAIDGAGNLFIC"
BASE COUNT          550 a          678 c          574 g          536 t
ORIGIN      
        1 cgcaaatgaa taagtttccc gccccgtcaa tcgcaattcc gtgcggatac cctaactgcg
       61 cctgagttgc cagcccatta tcaccaccaa acccaaaccc gccaaaccct gccggaccgt
      121 ttccaacaat cgtcgaaata ccaccgcttg attgattgac cttgcgaatg cgctggtttc
      181 ccgcatcagt aatcagcaaa ttgtcatcag cgtcaatcac gacatcgagt ggataagaca
      241 accgggcagc cgtcgcagga ccaccatccc cactcaagcc ttcagctccc gacccggcaa
      301 cagtggtgat gatgctggtt tgggcatcca cccgacgaat tcgatgattc tggcgatcag
      361 caatgaagag cgttccctga ctatccacca cgacatttga gggtcgaaag agtccagcgt
      421 gggtcgccgg accgccgtcg ccactgtatt cacgttttcc agtgccagcc accgtggtga
      481 tgatgccagt ttgggcgtcc acccggcgaa tccgactgct gcgatcaaca atgaacaaat
      541 tcccagttcg atcaatcgca aagctatctg gattgattcc agcttcaatg gctggcccac
      601 catcaccacg aaagctcggg gaaccagtgc ccgcaattgt ggttaacaca ccggttcgaa
      661 gatcaatccg ccgaatccgg aaattatcat tgatgaggag attacctgcc tgatcaatca
      721 caactttgtc ggcagccaga tcagccgtaa cggccagcgt accatctgga ctgaatcctc
      781 ctcggcctgt cccagcaacg gtcgtgataa tgccagtttg agcatctatc cggcgaatcc
      841 gatgcgaagc gtctgcgaca tacacattgc cggaactatc aatcgcagtt gaggttgggt
      901 aatttacggt ggcggcggtt gccattcgtc catcaccaac tccctgcatg cctccgacga
      961 cagtggtgat ttcaccggct gtcagttcag aaagtggttt tggaatgacc gccaggctgc
     1021 gttgaaccag accacctcgg gtttgaatgg tgagtgtccg gttgccatac ccgcgttgtc
     1081 gtggaactcg aaaccggcac tcggtcgcgc tctcaacctg taaattctcc accagcgcat
     1141 ccccaagact gacaattgat tctggggcaa agtttttgcc ggtgacgatg acttcttcgc
     1201 caccttcggc tcggactgtt tcacgattaa catttgtgat ttcggggccg ccgacggtga
     1261 caattttcag cgcctgccgg tcttcagttg ctccaggatc gagcacaaac gtatccgtgc
     1321 cgcgaatcgt aacgctaacc ggctgatgaa aagtcacttc tggctgggcc agatcaagct
     1381 ttcgtgcgaa atccccaagc ggctgcgtta tcaaaaaggc accggtttca tcaatcacat
     1441 tcaaattgtc aacactgtca aaactgagtg aaatcggctt tgaaaactca attggctggt
     1501 cagattgcgc ggttatttca aatgcccgcg cagcagattg agtaccgaca gttgacacgc
     1561 ggcaggtcaa gccaccgctt tcagtttggg ttaactcaag caatcgcacg cggttattgt
     1621 cctgatccgc cacggccagg acccgtccgg ttgagtttaa cgccagtcca gccgggcgtg
     1681 aaaaccgggc ttcgcgacct gtgccatcaa gcacacctga ttgccctggg tttccagcca
     1741 gcagccagac acgtttggcg ctgaggttca caaagtaaat cgcatggtta tcagtatctg
     1801 ccagaaagag attgccgctg ctttcaaccg cgataccgcg tgggctgcga aaaaccatct
     1861 ggttgaaggc agcacttccc tggctatcaa aaactggggt tccagggtca cccgttccaa
     1921 gcacagtttc aacctgattg gtaaatccga ttttccggac acagtggttt aaagtatccg
     1981 cgacatacac tccaccctga actcgcccat caatgccgat tgcggttggt ccggcaaaca
     2041 gtgattgagt cacgcttccg tcttgcaaac cacgggcgcc acgttgaccg gcaaaaattt
     2101 tcaaggacgc tttcaacgca tccgtggctc gcaaaatggt gttttgggct ggattggtca
     2161 gataaagtcg tccattttga tcaaccttgg tttcgccggg gactttttca gccggaccgt
     2221 ttttgacgcg agtcaccaca acaaataccg aatccgtgat gttgccattt gaagccgtaa
     2281 cggtggcaaa tccctgacgg acacctcgca cccgtccggt ttgtgggtca acctcagc
//
LOCUS       DAOQDO010000946         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41644_length_2337_cov_2.083260, whole genome shotgun sequence.
ACCESSION   DAOQDO010000946 DAOQDO010000000
VERSION     DAOQDO010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41644_length_2337_cov_2.083260"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PKZ53_14620"
     CDS             complement(<1..978)
                     /locus_tag="PKZ53_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ41725.1"
                     /translation="MSTHPTPLLDHHTIEDNFRELEPALSDAEAILESNRCLFCFDAP
                     CTRACPTHIDVPKFIKKIASGNLIGSARTIFEANPIGASCARVCPVEALCVGACVENA
                     LAHQPIMIGRLQRYATDAAMKTGRDLFKAGESNGKSVGIVGSGPAGIACATYLARLGY
                     DVTIYERRALPGGLDTYGMAEYKMTQRVSLEEVQMVERLGVKILVNTEIGRDVSLADL
                     ESRHGAIFLGIGLGSTIKLGIPGEEVPGVYEALDFIERIKTREWAHVPIGKTVAVIGA
                     GNTAVDAATQAKRLGAEEVLMIYRRSEREMPAYSYEYELAKQDAIEFRWH"
     gene            complement(1241..2113)
                     /locus_tag="PKZ53_14625"
     CDS             complement(1241..2113)
                     /locus_tag="PKZ53_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNJ41726.1"
                     /translation="MSSRIIKCGLIQTTNAGSTDAPIEEIKRLNIEKNLALVEDAARQ
                     GVKILCFQEVFTTPYFCAEQQTRWYEAVEKIPDGPTVRLMQDVAKQHGMVLVVPIYEE
                     EITGIYYNTAAVIDADGKYLGKYRKNHIPHVAPGFWEKFYFRPGNLGYPTFETAFARI
                     GVYICYDRHFPEGARILGLNGAEIVFNPSATVAGLSEYLWKLEQPAHAVANGYFIGAI
                     NRVGTEAPWNIGEFYGQSYFCDPRGQFLATGPRDQDALVVADLDLDIIRQVRNTWQFF
                     RDRRPDSYGAIVQG"
     gene            complement(2118..>2337)
                     /locus_tag="PKZ53_14630"
     CDS             complement(2118..>2337)
                     /locus_tag="PKZ53_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41727.1"
                     /translation="LAGWTLTHGFLVVAVVPVVPGFLRAAVTRGGQVANPGFLDTLYT
                     YAWFVTFAIGFVLYFIFMRNHPSLIKES"
BASE COUNT          588 a          652 c          572 g          525 t
ORIGIN      
        1 atgccagcga aattcaattg cgtcctgttt ggccagttca tattcgtagc tataggcggg
       61 catttcccgt tctgaacggc gataaatcat caaaacctct tcggccccga gtcgtttggc
      121 ctgagtcgcg gcgtctacgg ctgtgtttcc ggccccaatc accgccaccg tttttccaat
      181 tggaacgtgg gcccactcac gggttttgat ccgttcaata aaatcaagtg cttcgtagac
      241 tcccggtacc tcttcgcctg gaatgcccag cttgatggtt gagcccagcc caatacccag
      301 aaaaatcgcc ccatggcggc tttccagatc agccagtgag acatcgcgcc caatttcggt
      361 gttgaccaga atcttgaccc ccaaccgctc gaccatctgg acttcttcaa gcgaaacacg
      421 ctgggtcatt ttgtattcag ccatcccata ggtatcgagt ccgccaggga gcgcccggcg
      481 ctcgtaaatc gtcacgtcat atccaaggcg cgccagatag gtggcacagg caattccagc
      541 cgggccagat ccgacaattc ccaccgactt tccatttgac tcgccggctt tgaaaaggtc
      601 acgtccagtt ttcatggcag catccgttgc atatcgttgc aaccgaccaa tcataatcgg
      661 ctgatgggcc agggcatttt cgacacaggc tccaacacag agagcttcaa ccggacacac
      721 ccgcgcacag gaggcgccaa tcggattggc ctcaaaaatc gttcgtgccg acccaattaa
      781 gttcccggaa gcaatttttt taatgaattt tggcacatca atatgggtgg gacaggctcg
      841 ggtgcagggg gcatcaaagc aaaacaggca gcgatttgac tctaaaattg cctcagcatc
      901 tgatagagca ggttcaagtt cccgaaaatt gtcttcgatg gtgtgatggt cgagcaatgg
      961 ggttgggtga gtggacatgg acacgactcc tcagtgtttt tctttctgca gtttcgggtt
     1021 gggggaaggt cttcttaaaa attgaagacc ggagaagcgt aagataaaaa taaatagttt
     1081 ttgggatcgc cgctaacctg ccagcaccag gcgactaact tgccagcacc aggcgactta
     1141 gcccggtaca acacatacca cagcggtgtc aagccaccgc actccacagg cgtcaagccg
     1201 ccgaaaatgg aaagcggtgt caagccaccg cactccacag ctagccttgc acgatagccc
     1261 catatgaatc aggccgccgg tcgcggaaaa actgccacgt attgcggacc tgccggatta
     1321 tatccagatc cagatcagcc accaccaggg catcctggtc tcgtggtcca gtcgccagaa
     1381 actgcccgcg aggatcgcag aaataacttt gaccataaaa ttcgccgata ttccacggag
     1441 cttcggttcc aacccgattg atggcaccga tgaaataccc attggccacc gcatgggcag
     1501 gttgttcgag tttccacagg tattctgaaa gcccggccac ggttgccgat gggttaaaca
     1561 caatttcagc tccattcaac cccaggatgc gtgccccttc gggaaaatgc cggtcataac
     1621 aaatatagac tccgattcga gcaaaggcag tttcaaacgt cggatagccc aaattccctg
     1681 gccggaaata gaatttctcc caaaacccag gcgcaacgtg cggaatatgg tttttgcgat
     1741 atttcccaag gtactttcca tcggcatcaa ttaccgccgc cgtgttgtag taaatcccgg
     1801 tgatttcctc ttcataaatc ggcaccacca gaaccatccc gtgttgtttg gcgacatcct
     1861 gcatcaggcg cacggttggg ccatccggga ttttctcaac cgcttcgtac cagcgggttt
     1921 gttgttcggc acaaaaatag ggagtggtaa aaacctcttg aaaacacagg atcttgacgc
     1981 cttgtctggc ggcgtcttcg accagcgcca ggtttttttc gatatttaat cgcttgattt
     2041 cctcaatcgg cgcgtccgtt gaaccggcat tggtggtctg aatgagacca catttgatga
     2101 tgcgggatga cataaactta ggactcctta atcagacttg ggtgatttcg catgaagata
     2161 aaataaagca caaatccaat cgcaaatgtg acaaaccagg cgtaggtgta gagcgtgtcc
     2221 agaaatccag ggttggccac ctgcccgccc cgtgtgacgg cagcccgcaa aaatccagga
     2281 acaaccggga ccaccgcaac cacaagaaag ccatgagtca gcgtccaccc agcaaga
//
LOCUS       DAOQDO010000947         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41670_length_2336_cov_2.682157, whole genome shotgun sequence.
ACCESSION   DAOQDO010000947 DAOQDO010000000
VERSION     DAOQDO010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41670_length_2336_cov_2.682157"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>2336)
                     /locus_tag="PKZ53_14635"
     CDS             complement(41..>2336)
                     /locus_tag="PKZ53_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41728.1"
                     /translation="GSFRNEENEASQFLQGFFSTQSSLDKQFPFWEIDLQRLSRVEAE
                     QLTTLLLGKDGSDQQVEKIAQESDGSPFFIGVFAKHLVEKNLLHQTADENEKMTLERV
                     IGSQVESLSVPERKVLEVIAVAGQPVTYRVAQEASHLESFENLFSILRNSHLLRSTGQ
                     SGLQEIDTYHDKIREVVIRHLEPELVKGHHHSLGQALEGATEIDLERLAKHFRLAEEM
                     EKAFDYTLRAARQAEKSLAFERAVELYRQISSLKVAIEPAQVREIQIGMANALANAGR
                     GAEAARGYLSAVAYCNGVEQLQLQRKAAEQFLNSGQIGQGMEVLHQVLEKVGVRLLTG
                     NVEILLSIAIGRAKLWLRGMNYQERHPPEIGDQELLRITALFTTIDSLVGVNPLQAYE
                     LQTKHLLLALQAGEPYRLTKALMYESIFSGFSGTRKNQSSLQFIKMAEDLAHRTANSE
                     LCAMVHFGRCVTSFARGNWNAAWHSFRVGEEITQKECTGEDNGSAIRGIENVVTWGLR
                     SLFYQGDVNQLREKLPKYLVIGKARDNLLMLTNLGTYVVYLNHLVEDRPEVALDELER
                     TSNLWSQHSFHSHDYWKLIGKSEIGLYDSNPGPAWAEMNTRWPKILGWGIRSIQMVFI
                     ETLHAQARLALSMAQHQRNANAFLKIAENHARKIKREKIAYGDGWAELIFAGIEATRG
                     RTAAAISHLHSAEIKLAGADMRLYATAASRRRGELLANDEGKELINRSEAWMIGQQIK
                     NPARMADMLAPGKWNASDAKYKSP"
BASE COUNT          547 a          616 c          516 g          657 t
ORIGIN      
        1 gcgaaagcca atgcggagga gccgaatttc catcccttat ttatggggac ttgtacttag
       61 cgtcagaggc gttccactta cctggagcca gcatatcggc cattcgagcg gggtttttga
      121 tctgctgccc gatcatccac gcttcagacc ggttgataag ttcttttcct tcatcgttgg
      181 caagtaactc tccacgcctg cgacttgcgg cggtggcata gagtctcata tcggccccag
      241 ccaatttgat ctctgcggaa tgtaaatggc tgatcgcagc ggcggttctc cctcgggtag
      301 cctcaatccc ggcgaagatt aattctgccc agccatcacc ataggcgatc ttttcccgct
      361 ttatttttct ggcatggttt tcggcgatct tgagaaaggc attggcattc ctttgatgtt
      421 gcgccattga caaggccagg cgtgcctgag cgtgtaaggt ctcgataaac accatttgaa
      481 tcgatctgat tccccaaccc agtattttag gccacctggt attcatttcc gcccaggccg
      541 ggcctggatt tgagtcataa agcccaatct cggactttcc gattaacttc cagtagtcat
      601 gactgtgaaa gctgtgttgg ctccacaaat tactggttcg ctctaattca tccagggcga
      661 cttccggcct gtcttccacc aaatgattca ggtagacaac ataggtccct aaatttgtca
      721 gcatcaataa attgtcccgg gcttttccaa tgaccagata tttcggcagt ttttcccgta
      781 attggttcac atccccctga taaaaaaggg atctcagtcc ccacgttacg acattttcga
      841 ttccccgaat ggcggatcca ttgtcttctc cggtacattc tttttgggtg atttcttctc
      901 caacacgaaa ggaatgccag gcggcgttcc aattgccccg agcaaatgaa gttacacacc
      961 ggccaaaatg aaccatggca cataattccg aattcgccgt ccggtgggcc aaatcttcag
     1021 ccattttaat aaattgtaat gacgattgat ttttgcgcgt gccgctaaaa ccggaaaaga
     1081 tgctttcgta catcaaggct ttggtcagtc ggtagggctc acccgcctgt aatgccaata
     1141 aaagatgttt ggtttgcaac tcatacgcct ggagtggatt gactccgacc agtgaatcaa
     1201 tggtcgtaaa gagcgcggtg atccgcaata attcctgatc tccgatttct ggtggatgtc
     1261 tttcctggta attcatccct ctcaaccaga gttttgcccg gccaatcgcg atggataaca
     1321 ggatttccac attcccggtc aagagccgaa ctcctacttt ttccaatacc tgatgaagca
     1381 cctccatacc ttgcccaatc tgcccggaat tgagaaattg ttcagccgcc ttccgttgta
     1441 actgcaattg ttcgactcca ttacaataag ctacggctga taaatatccc ctggctgctt
     1501 cagcacctct cccggcattt gccagagcgt ttgccatccc aatttgaatc tctcgtacct
     1561 gggccggttc aatggctact tttaaacttg aaatctgtct atagagttca accgcccgct
     1621 caaatgccag ggatttttcg gcctgtctgg ctgctcgaag tgtgtaatca aaggctttct
     1681 ccatttcttc agcgagccga aaatgttttg ccagacgctc taaatcaatc tctgtggctc
     1741 cttccagcgc ctgacccaaa ctgtgatgat ggcctttgac taattctggt tctaaatgtc
     1801 ggatgacaac ctcgcgaatt ttatcgtgat aggtgtcaat ctcctgaagg ccgctttgac
     1861 cggttgagcg gagtaaatga ctgttccgca agatcgagaa aagattttca aagctttcca
     1921 gatgactggc ttcttgagcc acccgatagg taaccggctg gccggcgacg gcaatgactt
     1981 ccaatacctt gcgctcaggg acagataagc tttctacctg cgaaccaatc acccgctcaa
     2041 gggtcatttt ttcattttca tcggctgttt gatgaagcag gtttttctcc accagatgtt
     2101 tggcgaatac accaataaaa aatggactcc cgtccgattc ctgggcaatt ttttcaacct
     2161 gttggtcact gccgtccttt ccgagtaaga gtgtcgtcag ttgctcagct tcaaccctgg
     2221 acaatcgttg taaatcaatt tcccagaatg gaaactgctt gtccaggctg gattgagtcg
     2281 aaaaaaagcc ttgaagaaac tgactggctt cattttcctc attccgaaag gaaccg
//
LOCUS       DAOQDO010000948         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41748_length_2333_cov_2.796313, whole genome shotgun sequence.
ACCESSION   DAOQDO010000948 DAOQDO010000000
VERSION     DAOQDO010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41748_length_2333_cov_2.796313"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKZ53_14640"
     CDS             <1..604
                     /locus_tag="PKZ53_14640"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HNJ41729.1"
                     /translation="NNMSEELEQIENEAHPIVSQVASHHLRAALKSLRLTAVADEHLA
                     RTPENVAEFWMEFFAPYLSHAAPPVIPTFPAQEMAGQCVLIKDLAYHSMCAHHLTPFF
                     GEAHIAYVPHELGIGIGSPAKLLEFYARRPQLQERLAVQLADGLIEACQPRGVMVFLT
                     ARQMCMEMRGTKTKGVVECFVARGCFEEDSWRQLFFARLK"
     gene            complement(908..1600)
                     /locus_tag="PKZ53_14645"
     CDS             complement(908..1600)
                     /locus_tag="PKZ53_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ41730.1"
                     /translation="MSQHILLIEDEPGLVLTLTDRLESEGYTLETARDGEAGLHLAQT
                     KPFDAIILDIMLPRKNGFDVCRDLRQQGNRTPILMLTARGQVVDKVVGLKLGADDYLT
                     KPFEMMELLARVEALLRRVQPGTSASGGTEAFQFDAVQVDFKRAEVLRNRLPVELSAR
                     EFKLLQYLIEHRGRTISRDELLDEVWGYNAMPSTRTVDVHIAWLRQKLEPNPRHPRFI
                     LTVHGLGYKFAG"
     gene            complement(1569..>2333)
                     /locus_tag="PKZ53_14650"
     CDS             complement(1569..>2333)
                     /locus_tag="PKZ53_14650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ41731.1"
                     /translation="RQQMEFVAAVSHELRTPLAVICSAAENLADGVVWNPQRVAQYGT
                     LIRREGRRLTEMVEQVLELAGVHSGRRTYHLAPVEVRTLVESALIPYAPLLDEGNFSV
                     EVELESELPFLYGEMAALRRALQNLLSNAMKYSGDQRWIRITARLYKTESRSEIWLAV
                     TDKGVGIKPGDVPHIFDQFFRSSEAVEAQIHGSGLGLSLVKHIVEAHGGHISVQSQPG
                     LGSTFTLHLPVTSVVIPEKPDSIEDVYEPTHFAHRR"
BASE COUNT          578 a          601 c          574 g          580 t
ORIGIN      
        1 aaataacatg tctgaagaac ttgagcaaat agaaaatgaa gcccacccga ttgtctcaca
       61 agtggctagt catcacctgc gtgccgccct gaaatcgctt cgtctgacgg cggtggcgga
      121 tgagcatctg gcgcgaaccc ccgagaacgt ggctgaattc tggatggaat tttttgcccc
      181 atatctttcg cacgccgcac cacccgtcat tcccacgttt ccagctcagg aaatggccgg
      241 ccaatgcgtc ttgatcaaag atctcgccta tcattcgatg tgtgcccatc acctgactcc
      301 gttttttggt gaagcacaca ttgcctatgt tcctcacgaa ctgggaattg ggatcgggtc
      361 accagctaaa ttgcttgagt tctatgcccg tcggcctcaa ctgcaagaac ggcttgctgt
      421 ccaactggca gatgggctga tcgaggcttg ccagccacgt ggcgtgatgg tcttcctgac
      481 ggcacgccag atgtgcatgg aaatgcgagg caccaaaacc aaaggggtgg tggaatgttt
      541 tgtggcccga ggttgctttg aagaagattc ctggcgacag ttgttttttg cccggctcaa
      601 atgaatgatg aatgaagaat gaagaatgaa gaaaccattg agtggttggt gatgagtgat
      661 gcgaattaag cattgaaatt aagatttcag aatcaacaac ttacagacag taaattgaac
      721 atctgaaatt tgagattttg ataagccttg ttttcaacga acaattgttt caatccttaa
      781 ctggcattcg tgcttagtgg tgagaaatca atactttcga agaaaaacca agtaccagga
      841 accagatacc aacttcattc ttcattcttc attcttcatt cttcattctt cattctcaat
      901 tcttgattca tccagcaaat ttatagccca gtccatgcac ggtcaggata aagcgtgggt
      961 gacgggggtt gggttcgagt ttttgccgga gccaggcgat gtggacgtca accgttcggg
     1021 ttgagggcat cgcattatag ccccagactt catctaacag ttcatcgcgc gagatggttc
     1081 gccctcgatg ctcgatcaag tattgcaata acttgaattc acgtgccgac aattcaaccg
     1141 gaagccggtt tcgcagaact tcagcacgtt tgaaatccac ctgaaccgca tcaaactgga
     1201 aagcttcggt tccccctgat gcagatgtac cgggttgaac tctccggagt aaagcctcga
     1261 ccctggccag taactccatc atttcaaatg gtttggtaag atagtcatca gcacccagct
     1321 tgagtccaac aactttatcc accacctgtc cccgagccgt gagcatcaaa atcggggttc
     1381 gatttccctg ctggcgtaaa tctcgacaca cgtcaaatcc atttttgcgt gggagcatga
     1441 tgtccagaat gatggcatca aagggtttcg tttgtgccag atggaggccg gcttctccat
     1501 cgcgggctgt ctcaagggta tatccttcac tttccaggcg gtcagttaat gtcaggacca
     1561 gccccggctc atcttcgatg agcaaaatgt gttggctcat aaacatcctc aatcgaatcc
     1621 ggtttctcag gtatcaccac ggaagtgact ggcaaatgca gcgtaaaggt actgccaaga
     1681 cctggttgac tttgaacgct gatatggccg ccgtgggctt cgacaatatg tttgaccagg
     1741 ctgagtccga gaccactccc atggatttgg gcttcaactg cttctgaact tcgaaagaac
     1801 tggtcgaaga tatgcgggac atcaccgggt ttgataccca cccctttgtc agtcaccgcc
     1861 agccagattt cagaacggga ttccgtcttg tagagacggg cagtaattcg gatccaccgc
     1921 tggtcaccgc tgtatttcat cgcattgcta agcaggttct gaagcgctct gcggagtgcc
     1981 gccatctcac cataaagaaa aggcaactct gactcaagct ctacttcgac tgagaaattg
     2041 ccttcgtcca ggagcggggc atagggaatc aacgcacttt caaccagggt tcggacctca
     2101 accggtgcca ggtgataggt tcgtcgcccg gaatgaacac cagccagttc caaaacctgt
     2161 tcaaccattt cggtcaaacg gcggccttcg cgccggatca aggtgccgta ttgggccacc
     2221 cgttgcgggt tccacaccac gccatcagcc agattttcag ccgcagaaca tatgacggcc
     2281 agcggagtgc gaagttcgtg tgagactgcc gcgacaaact ccatttgctg acg
//
LOCUS       DAOQDO010000949         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41831_length_2331_cov_2.717926, whole genome shotgun sequence.
ACCESSION   DAOQDO010000949 DAOQDO010000000
VERSION     DAOQDO010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41831_length_2331_cov_2.717926"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKZ53_14655"
     CDS             complement(<1..407)
                     /locus_tag="PKZ53_14655"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ41732.1"
                     /translation="MLWWTLQELKSSNVETRLRAARKLGTTRDQRAIEALILALKDRE
                     FLVRNAAEEALNAIDPNWLRSSTARSAVPAFIAALNEQDREIRKSAAYVLGTICDLKA
                     SDALIARLADHDRDVRKAAAESLGMMRSKKAVPA"
     gene            637..966
                     /locus_tag="PKZ53_14660"
     CDS             637..966
                     /locus_tag="PKZ53_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNJ41733.1"
                     /translation="MSTKPNLTYASALILQVIVNGYQYGFDIMDVTGLPSGTVYPALR
                     RLEQSGLIASQWEAAEIAQQELRPPRKYYEVTSVGTQTLTEAVKRFRLLETMIPNLPR
                     KERPSEG"
     gene            972..>2331
                     /locus_tag="PKZ53_14665"
     CDS             972..>2331
                     /locus_tag="PKZ53_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ41734.1"
                     /translation="MLKPESPSEFKLKLLLSLISTVAVVVPRHQRADWKQEWQAEIIF
                     HWRQVHQRPHFNQKTRFKVLNYCNGVVLDALVLRTESSDLFQWIGDVAQGMAAVVRSP
                     RTALVSSGMLSAGVSCLMVGLAVVGASLWLPLPMAQADGLVVVNNTCSVIESKTLPFS
                     VSEFYDLQKSVTSLQAIEAIHFSLANVSFPHTSMAVKLGRVSDGFLSLTSGGAALGSC
                     QWSSSRNLSHSLPGVVISHRFWKQYLDARPDVLGTRLEVDQTPYQIQGVMPAGFDVPG
                     QINLWIQLVPPAHLGTQTERDTRFLTILGRLKPGMTINQVQQELDLLARRLKPTPPET
                     GSVQWPVWGMTVRSARQWWLGDWYETILWLFVGTVSVFGLAVWATIRTEGTPSIEAEF
                     STQSLRRFRWSLGEGWGLMVLGMIGGGLAGIWYLKLVNHVFLQPVLNIEPARLDGWVW
                     GTT"
BASE COUNT          527 a          582 c          630 g          592 t
ORIGIN      
        1 gccgggacag cttttttgct gcgcatcatc ccgagcgatt cggctgcggc tttacgcaca
       61 tcccgatcat ggtcggcaag cctggcaatc aaggcatctg aggctttcag atcacaaatg
      121 gtgccaagca cataggccgc cgacttgcga atttcgcgat cctgttcatt taaagccgcg
      181 ataaaggctg ggactgccga acgggcagtt gaggatcgca gccagtttgg atcaatcgca
      241 ttgagtgctt cttcagccgc attgcgaacc aaaaactccc ggtctttcaa cgccagaatc
      301 agggcttcga tagcgcgttg gtccctggtt gtgcctaact tgcgggcggc tcgtaaccgg
      361 gtttcgacat tgcttgactt cagttcttga agcgtccacc acagcataac cggcgcactc
      421 cttccaattt ggaagcaaaa aaacggggat aatgaattgg ggaaaccaca gttgagatga
      481 aaaaggtgcc ccattatgcc tctggttgtg acttgtcaca aggttttgtt cagcagatat
      541 cagaagacct tcttgacagt tcctgccaga atggcttata tagatagaaa actacatagg
      601 tagaatcgta tatagaacca ttgaggtcaa ttcactgtgt ccaccaaacc aaacctgact
      661 tacgcctctg cgctcattct gcaagtcatc gtcaatggct atcaatatgg ttttgatatt
      721 atggatgtga ccgggctgcc aagtggcact gtctacccag cgttgcgccg tctcgaacaa
      781 tctggactga ttgcttccca gtgggaagcc gctgaaattg cccagcaaga actccgccca
      841 cctcgaaaat attatgaagt gacctccgtc ggcacacaaa cactcactga agctgtcaaa
      901 cggtttcgat tacttgaaac catgattcca aacctgccac ggaaagagcg accatccgag
      961 gggtaatcgg tatgctgaaa ccggagtcac catcggaatt caaactcaag ttgctcttgt
     1021 cgttgatttc aaccgttgcg gtggtggtgc ctcgacacca gcgagccgat tggaaacaag
     1081 agtggcaggc cgaaattatt tttcactgga gacaggtgca ccagcggccc catttcaatc
     1141 agaaaacgcg tttcaaggtc ttgaattact gcaatggggt ggtgcttgat gctttggttt
     1201 tgagaactga atccagtgac ctgtttcagt ggatcggaga tgtcgcccag ggcatggcag
     1261 cggttgttcg ctccccgcga accgccctgg tttcaagcgg tatgctttct gccggggtta
     1321 gttgtttgat ggttggtctg gctgtggttg gcgccagtct ctggttgcca ttgcctatgg
     1381 ctcaggcgga tggcctggta gttgttaaca acacctgttc cgtcatcgaa tccaaaacgc
     1441 ttccgttttc ggtttctgaa ttttatgacc tgcaaaaaag tgtgacgtcg ctgcaggcca
     1501 tcgaagccat ccacttttcg ctggcgaatg tttcatttcc acacacctca atggccgtga
     1561 aactaggccg ggtctcagat ggatttttat cactcacgtc aggtggggct gcacttggaa
     1621 gctgtcagtg gtcgtcatcg cgcaatctat ctcactcgct gccaggtgtt gtcatcagtc
     1681 accgtttttg gaagcagtat cttgatgccc ggccagacgt acttggtacc cgccttgaag
     1741 ttgatcaaac gccgtatcag attcaaggtg tgatgcctgc cggttttgat gttcctggtc
     1801 agatcaacct gtggatccag ttggtacccc cggctcatct gggaacccag actgagcggg
     1861 acactcgatt tctcactatt ttggggcgat tgaaacctgg aatgaccatc aaccaggtgc
     1921 aacaggaact cgacttgctg gctcggaggc tgaaaccaac gccgccagag actggctctg
     1981 tccaatggcc agtatgggga atgacggtca ggagcgcgcg ccagtggtgg ctaggtgact
     2041 ggtacgagac catcctctgg ctgtttgtcg gaacggtgag tgtctttggt ctggcggttt
     2101 gggccaccat ccgaaccgag ggaacaccat caattgaagc tgagttctca acccagtcac
     2161 tccgacgttt ccgctggagt ctcggtgaag gttgggggtt aatggttcta gggatgatcg
     2221 ggggcggcct ggcaggaata tggtatttga agctggtcaa tcacgtgttt ttgcaaccgg
     2281 ttttgaatat agaaccagcc cggcttgatg ggtgggtgtg gggaacgacc t
//
LOCUS       DAOQDO010000950         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41850_length_2330_cov_3.840440, whole genome shotgun sequence.
ACCESSION   DAOQDO010000950 DAOQDO010000000
VERSION     DAOQDO010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41850_length_2330_cov_3.840440"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1526)
                     /gene="pelF"
                     /locus_tag="PKZ53_14670"
     CDS             complement(63..1526)
                     /gene="pelF"
                     /locus_tag="PKZ53_14670"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HNJ41735.1"
                     /translation="MVHTVLLATEGTYPFHGGGVSTWCNVLLHRLEEFQYRVLSVIMH
                     PYLPPRYDPPPNLKSIIKVPLWGTEDPAEQQFDRTFATILNRRRQTTEPMIRRHFLPI
                     FEALLDDITGNATGAVTGKRFADMAAYFRQFDYFETFRSRVIWYSFQDFLRAWVRSAT
                     PALPSPTVRDAAECLQLLTRLLLVLDVEVPRTSLTHSAAAAVCGLPCVVAKLRQGTPY
                     LLTEHGVYLREQYLNVARTARSTIVKRFLLGFFGAVVRANYHYADVVSPVCQYNARWE
                     LAQGVAPDRLQVIYNGSNETRFCPLDLPRRHRPTVTQVGLIFALKGTKDLIQAAAQVR
                     AAVPEVEFLLYGSASDPAYFAECQTLTTQLGLEQTVTFAGLTSHPEQVFNQADVVTLS
                     SISEGFPYTIVEAMLCGSAIVATNVGGVGEALADTGLLVPPRTPDQLAQAIVKLLTNA
                     HLRRDLGQRARERALTHFTESQFATHYRDLYQQLIGG"
     gene            complement(1633..>2330)
                     /locus_tag="PKZ53_14675"
     CDS             complement(1633..>2330)
                     /locus_tag="PKZ53_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41736.1"
                     /translation="RAAITEGLASFVQPERFVGYLTCAEVLPNDPILKQAFPQDYYRV
                     NGVPVRIPGSNAYLMDLRQSHWRTLLINQMQKLISSGYDGVFLDTLNIGDEPPYAGTP
                     TGEGFLQSAGFLTRDMRTRYPNNLILQNWGINRLKTFTAGYLDAICWENFQVQYIETD
                     AWSQARLAELTALQGGGLSVMTLYQLTSAQITDTAYQSRMYQVSRSYNFLPYCALPGY
                     TSGVNLLPLQNGF"
BASE COUNT          559 a          606 c          651 g          514 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac cctatgttgt
       61 ctttaccctc caatcaactg ctggtacagg tcgcgatagt gggtggcaaa ctggctttcg
      121 gtaaagtgag tcaacgctcg ttcacgggca cgctggccaa ggtctcttcg caagtgggcg
      181 ttggtcaata atttcacgat ggcttgagca agctggtccg gggtccgggg tggcaccagg
      241 agcccggtat cggcgagtgc ttcaccgacg ccgccaacat tggtggcgac gatggcgctt
      301 ccgcagagca tggcttcaac gatagtgtag gggaatcctt cggaaatgct gctcagggtt
      361 acgacatccg cctgattaaa aacctgttcc ggatgcgacg tcagtccggc aaaggtgacg
      421 gtttgttcta agccaagttg agtcgtaagt gtctgacatt cagcaaaata ggccgggtcg
      481 ctggctgatc cgtacaataa aaactcgact tctgggacgg cggccctgac ctgagccgcg
      541 gcctgaatca gatctttggt acctttgagg gcaaaaatca aacccacctg ggtgacggtt
      601 ggtcggtgcc gtcgcggaag atcaagcggg cagaatcggg tttcattgga cccattatag
      661 atcacctgca atcggtcggg ggctaccccc tgagccagtt cccagcgggc attgtactga
      721 cacacgggtg ataccacatc agcataatgg tagttggcac gcaccactgc cccaaaaaac
      781 ccaagcaaaa accgtttgac gatggttgat cgagccgttc gggcaacatt cagatattgt
      841 tcgcgaaggt acacgccatg ctcggtgagc agatacgggg tgccctgtcg cagtttggcg
      901 accacacagg gcaggccaca cactgcggca gcggctgaat gggtcagtga tgttcgcgga
      961 acttccacgt ccagaaccag caacaaccgg gtcagcagtt gaaggcattc cgctgcatct
     1021 ctcacggttg gggaaggcaa cgcaggcgtg gctgacctga cccaggcacg tagaaaatcc
     1081 tgaaaactgt accagataac ccgcgaccga aaggtttcaa agtaatcaaa ctggcggaag
     1141 taggccgcca tatcggcaaa ccgtttcccg gtgacggcgc cggtggcatt gcccgtgatg
     1201 tcgtcaagca atgcttcgaa gatcggcaga aagtgacgcc gaatcatcgg ctctgtcgtt
     1261 tgtcgccgcc ggttgagaat ggtggcaaaa gtgcggtcaa actgttgttc agccgggtct
     1321 tcggttcccc aaagcggaac tttgatgatg cttttgaggt tgggtggcgg atcataccgg
     1381 ggaggcagat aggggtgcat aattaccgac agtacccggt actgaaactc ttccagccga
     1441 tgcaacaaca cattgcacca ggtactgact cccccaccat gaaatggata ggtgccctca
     1501 gtcgccagaa gtactgtatg aaccatgccg gagtaattgc ttgccaccac gatgaagcta
     1561 tctggtcctt agagttcacc tgttcgaaga aaagaatttc gatttgtatt ctgatccctg
     1621 acccctgaca ctctaaaacc cattttgtag cggcagcagg ttgaccccag atgtgtatcc
     1681 aggtagggcg cagtagggaa ggaagttata gctacgtgac acctgataca ttcgtgactg
     1741 ataggcggtg tccgttatct gggcactggt tagttgatac agcgtcataa ctgacagtcc
     1801 gcccccttgc agtgcggtca actctgccag gcgagcctgt gaccaggcat cggtttcaat
     1861 atactgaacc tggaagtttt cccagcagat cgcgtccaga tatccggcgg taaaagtttt
     1921 aagccggttg attccccagt tttgaagaat caagttattt gggtaacggg ttcgcatatc
     1981 gcgtgtcaga aaaccagccg attgtagaaa accttcgccc gttggtgtgc cggcataggg
     2041 tggctcatcc ccaatgttca aggtatcgag aaacacaccg tcatagccgc tactgatcaa
     2101 tttttgcatc tggttgatca ggagtgtccg ccagtggctt tgccgcaaat ccatcagata
     2161 ggcatttgaa ccaggaatgc gcactggcac accgttgact cgatagtaat cctggggaaa
     2221 cgcctgtttc agaataggat cattgggcaa tacttcagca caggtcagat agccgacgaa
     2281 gcgttcgggc tggacaaagc tagctaaacc ttcggttatt gctgctctgc
//
LOCUS       DAOQDO010000951         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41880_length_2329_cov_3.821020, whole genome shotgun sequence.
ACCESSION   DAOQDO010000951 DAOQDO010000000
VERSION     DAOQDO010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41880_length_2329_cov_3.821020"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKZ53_14680"
     CDS             complement(<1..881)
                     /locus_tag="PKZ53_14680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41737.1"
                     /translation="MAVVVDIIGKVLDKKYLIQKILGQGGMGAVYQATHIGTRRPVAL
                     KIIMPRFMNEVEFVERFKREARASGQLIHPHVVNVTDFGFAAIGSEQVAYLVMEYLKG
                     QTLAELLASYPQLPLKTTVEIVEQVCLAIQEAHHRGIVHRDLKPENIFLQDDGRGGFL
                     VKVLDFGIAKLNERELLPSSEPDPHTTETQSLVDNTLAGGSLPQVTEKTEMWVGQVEE
                     ATPYLMEMTRYQGSLAPNETLVRGADGNSGPDDQQTGQFASPAGSKLAELTLKFSHPP
                     SGQNLSSPQQTLNSLSAP"
     gene            complement(1149..1583)
                     /locus_tag="PKZ53_14685"
     CDS             complement(1149..1583)
                     /locus_tag="PKZ53_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNJ41738.1"
                     /translation="MTDWLQREIILAPKPRGFHLITRETLRQIPELETFTIGLVHIFL
                     CHTSASLTINENASPDVRRDFESHFNVMVPENARYYRHTDEGPDDMPAHLKASILGSS
                     VTLPLTRGRFNLGTWQGIYLCEHRNDGGSRRLVVTIHGQMAR"
     gene            1781..2146
                     /locus_tag="PKZ53_14690"
     CDS             1781..2146
                     /locus_tag="PKZ53_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNJ41739.1"
                     /translation="MSNLYEELGGQGAVDAAVEMFYRRVLVDDRISHFFDGVDMDHQI
                     AKQKAFLTMAFGGPHNYSGLDMRKGHAHLVERGLNDTHFDAVVENLGATLAELGVRQE
                     LIDQVAAIAESTRADVLSR"
BASE COUNT          560 a          548 c          606 g          615 t
ORIGIN      
        1 ggcgcagata aagagttcaa cgtctgctgg ggagacgaca gattctgccc agatggcgga
       61 tgtgagaatt tcagggttag ttcagccaac tttgaacctg ctggtgaggc aaactgaccc
      121 gtttgctggt catctgggcc ggagtttcca tcggcaccac gcaccagtgt ttcatttggt
      181 gccaggctgc cctgatagcg ggtcatttcc atcaaatatg gagtggcttc ttcaacctgt
      241 ccaacccaca tttcagtttt ctcagtcacc tggggcagac ttcctcccgc gagtgtattg
      301 tcaaccagcg actgggtttc agtggtgtgg gggtctggtt cggacgacgg cagcagctcg
      361 cgttcattga gcttggcaat cccaaaatca agcactttga ccagaaagcc acctcgacca
      421 tcatcctgca gaaagatgtt ttcaggcttc aaatcacgat ggacaattcc ccggtgatgg
      481 gcttcctgga tggccagaca gacctgttcg acaatttcaa ccgtggtttt gagcgggagc
      541 tggggatagg acgcgagcaa ttcggccagg gtttgtcctt tgagatattc catgaccaga
      601 taggccacct gctctgaccc aatagcggca aacccaaaat cggtcacatt gaccacgtgg
      661 ggatggatca actgcccgga ggcacgagct tcgcgcttaa atcgctcgac aaattcaacc
      721 tcattcataa acctgggcat aataattttg agcgcaacag gtcggcgcgt tccgatgtgg
      781 gtggcctggt acacggcacc catccccccc tgcccaagaa tcttttgaat cagatacttt
      841 ttgtccaaca ctttaccgat gatgtcgacg acgacggcca tataaaatcc tagattgatg
      901 tgaaattgat gaagggggga ggatggaatg aagaatcaag aatcaaaaag ccactatcta
      961 attgaacatc aaatagttag ctgctctttg atccattctt cataaatgaa gagagagcat
     1021 gctgaagctg attactctta attcctaccc ctggttgaat tattcccagt tgcttacggg
     1081 agattcgaac aatttcgatg ccttggccga ttatcaaatc tggtgatgac catctgtatg
     1141 ggacctggtt acctggccat ctgtccgtga atggtgacga ccagccggcg ggaaccaccg
     1201 tcgttgcggt gttcgcacag gtagattccc tgccaggtgc cgagattgaa ccgcccacgc
     1261 gtcaggggga gtgtcacgct actgcccaaa atcgaagctt tcaaatgggc aggcatatcg
     1321 tctgggcctt cgtcggtatg ccggtaatag cgcgcgtttt ccggaaccat gacattgaaa
     1381 tggctttcaa agtcgcgccg tacgtctggc gaggcatttt cattgatcgt cagtgacgct
     1441 gacgtatggc acagaaaaat atgaaccagc ccaatcgtaa aggtttcgag ttcggggatc
     1501 tggcggaggg tttcacgggt aatgagatgg aacccccgcg gtttcggggc caggataatt
     1561 tcacgttgaa gccagtcggt cataggctga gagattcctt tgatctgagg gttttggggc
     1621 attatgccgg gccaaatctg aaaatgaaac ctgtttgcgg cttttgattc tcggactggc
     1681 gcggaaaaag aagattgact cagctcagaa aatatgtatt ctaaatacaa ctttaatttg
     1741 ccgctgtttt gcttactttt taaatttgag gtatgtgtca atgtcaaatc tgtatgaaga
     1801 attaggtggt cagggcgcgg ttgatgccgc ggttgaaatg ttttatcgaa gagtcttagt
     1861 tgatgatcgg atttcccact tttttgatgg ggttgatatg gatcatcaaa tcgccaaaca
     1921 aaaagcattt ttaaccatgg cgtttggcgg tccccataac tactcggggc ttgatatgcg
     1981 caaaggccac gcccatctgg ttgaacgcgg gttaaatgac actcactttg acgcggtggt
     2041 tgaaaatctc ggcgccaccc tggctgagct tggcgttcgg caggagttga ttgaccaggt
     2101 cgcagcgatt gctgaatcca cgcgtgcgga tgtgctctcg cggtaagtgt ttctgccccc
     2161 ggatccctct gcttgctgag tgatccgcca gttgggtttc gatactttgt aatcgaagcc
     2221 caactgtttg tttctggctc gttagcgtga ttgtaattct atttcctcta acttataagt
     2281 accttaccga aaatttccag acattttgaa ccacgaaata cacgaaata
//
LOCUS       DAOQDO010000952         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_41913_length_2328_cov_3.462385, whole genome shotgun sequence.
ACCESSION   DAOQDO010000952 DAOQDO010000000
VERSION     DAOQDO010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41913_length_2328_cov_3.462385"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKZ53_14695"
     CDS             <1..807
                     /locus_tag="PKZ53_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41740.1"
                     /translation="ITGIIEYGLGTPLLGTSWITLRWISGCVGLLGIAIVMTYPIRRQ
                     IWKHRVGPLRYWMLGHTYAGVIAGLVILLHGGTSLGGLLTTALMISFDLVILTGLVGI
                     LIYQFGPRLLTQIEEEPLLLEDLVRRREELYQEIADLLETAQSEARKHHTESEFIAFF
                     LPARDRVLKLTTSLSFLFRNYLYREPLDTLLEAMSWVFQPDQNQLKTKSEQVIFKRIV
                     EAAVTARRIDSLVYLHRALRLWLPPHVITTSFMLVLLLAHIFQVIYYLWR"
     gene            1016..>2328
                     /locus_tag="PKZ53_14700"
     CDS             1016..>2328
                     /locus_tag="PKZ53_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ41741.1"
                     /translation="MLSRYIIRKQDWGRHELSIERDGLTIGSAFENDLLLNHPTVSRT
                     HAGICEVAGLFWLTNLSKSNGTLVNGKLTDLTYLDTGDTIQIGVFLVRTTVRQHELIL
                     EVERYFDSFTAGNRGAQLLTDPGLTITEGPATVLMENGAAIVAAALNAPAITPEAQEA
                     PTLDLGPLAERSVRLRAVGKSLANSSEIKQVFQDLIQEVDLLFDSVVLPGVKDPNLDQ
                     WFSELIQEIDLIFEKLDSSDGRQRVVEISAASQNAEKVMIQVSDLQKLLARQREQGAA
                     LQVSYQGKIVPVQPTSRNSTLLLNKARLQRDLAGLLTSIIALPDEDRKDFQQAMTVFW
                     ERRKQAIENKQKLHDESVLRPQKPDPVLEEHNLYLGKWRFHWLPTSDLTMPWPRGYLF
                     TVGAVTLVAVIIAIFAWEKMYSPGPVSSPHSLKAEELVDQSPLVM"
BASE COUNT          567 a          568 c          590 g          603 t
ORIGIN      
        1 attaccggga ttattgaata tggcctggga acgccgctgc tggggacaag ctggatcacg
       61 ctacgctgga tttcaggatg tgtgggcctg ctggggattg cgattgtcat gacctatccg
      121 attcggcgtc agatttggaa acatcgggtt ggtccgcttc gctactggat gctcgggcat
      181 acctatgccg gggtgattgc cgggctggtg attttgctcc acggcggcac cagtttaggt
      241 gggttgttaa ccacagcgtt gatgatttcg tttgatctgg tgattttaac tggattagtt
      301 ggtattttga tctatcaatt tggcccgcgc ttgctgactc aaatcgaaga agagccatta
      361 ttgcttgagg atctggtccg gcgccgtgaa gaactctacc aggaaatcgc tgatttactc
      421 gaaactgccc agagcgaggc ccggaaacat cacactgaat cagagtttat tgcctttttt
      481 ctaccagccc gtgaccgggt cctcaaactc accacgtcgc tttcattttt atttcgcaac
      541 tacctgtacc gcgaaccact cgacaccttg ctggaagcta tgagctgggt ctttcaacca
      601 gatcagaatc agctcaaaac caaatcagag caagtgatct tcaagcgcat tgttgaagcg
      661 gcggtgactg cccggcggat tgattcgctg gtctaccttc atcgtgcctt gcgtctctgg
      721 ttgcctccgc acgtcattac cacctcattt atgctggtgc ttttgttagc acatattttt
      781 caggtgattt attacctctg gcggtgatga gtggttagtg gttagtggtt agtggttaga
      841 aatcaatgct ttcgaagaag agccaaaaac caaaaaccaa aaaacaaaaa ccaagaacca
      901 agaaccaaga accaagaacc aagaaccaag aactaaccac taaccactaa ccactaacca
      961 ctaaccacat tctcaattct tcattctcaa ttcttcattc ttcattcggt tcgccatgct
     1021 ttcacgctac atcattcgaa aacaggattg gggacgccac gaactgagta tcgagcggga
     1081 tgggttaacc attggttcgg cctttgaaaa tgatttgttg ctgaaccatc caaccgtttc
     1141 acgaactcat gccgggattt gtgaagtcgc tgggttgttt tggctgacca atctttccaa
     1201 atcaaatgga acactggtca atggaaaact cacggatctg acttatttag acaccggaga
     1261 tacaattcag atcggggtgt ttctggttcg aactactgtt cgccagcacg agttgattct
     1321 ggaggttgag cgctatttcg actcgtttac tgctgggaac cggggcgccc agttattgac
     1381 tgatccagga ctgacaatta ctgagggacc cgcaactgtc ctgatggaaa atggggcggc
     1441 gattgtcgcc gcggctttga atgcacccgc aattacgcct gaggcacagg aagctccaac
     1501 gcttgatctg gggccactgg ctgaacgatc tgttcgactt cgggcggttg ggaaaagcct
     1561 ggccaattcc tctgagatta aacaggtttt tcaagacctg atccaggaag ttgatctgct
     1621 ctttgattca gttgtgttgc caggggtgaa agatccaaac ctggatcaat ggtttagtga
     1681 attgatccag gaaattgacc ttatttttga aaagctggat tcatccgatg gccggcaacg
     1741 ggtcgttgaa atttcggctg ccagccagaa tgccgaaaag gtcatgattc aggtgtcgga
     1801 tttgcagaaa ctcctggccc gccagcgaga gcaaggcgcc gcgctccagg tttcctatca
     1861 gggcaagatc gttccggtgc agcccacctc ccgaaattcg accctgctgt taaacaaggc
     1921 ccgattacaa cgcgacctgg ccgggttgct cacatcaatt atcgccctgc cggatgagga
     1981 tcgaaaagat tttcaacagg caatgactgt cttctgggag cgacgcaaac aggcgattga
     2041 aaacaaacaa aagttgcatg atgagtcagt gttgcggcct caaaagccgg acccagttct
     2101 ggaagaacac aacctctacc ttggcaaatg gcgttttcac tggttgccaa ccagtgattt
     2161 gacgatgccc tggccacgtg gatacctgtt tacagtgggc gccgtgactt tggtggccgt
     2221 gattattgcc atctttgcct gggaaaagat gtactcaccg gggccggttt ccagtcccca
     2281 ttcactcaag gccgaggaac tggttgatca atctccactg gtgatgag
//
LOCUS       DAOQDO010000953         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42044_length_2324_cov_3.139709, whole genome shotgun sequence.
ACCESSION   DAOQDO010000953 DAOQDO010000000
VERSION     DAOQDO010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42044_length_2324_cov_3.139709"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PKZ53_14705"
     CDS             complement(<1..1323)
                     /locus_tag="PKZ53_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ41742.1"
                     /translation="MNKRNNFIALSVFIALLAVAAGSRFSVTANSTAAQPVWQKSRQV
                     AVPLPQYAAFDLESASDLATVAAGPSVVRYADVDGDGIADLIAVKNGNLQLQRGQAAT
                     EFGLPEILETGSSTTALAVGDINGDGYPELLALNASNSTVTVWGQADQSQTVRGEANR
                     LPLSILAIQPVLPNATALAVVDVNQDDIQDVVVSAGDGLVLCLGTNDGLAAGQHMAAP
                     AGVSLNYLATAPSASTVWTVDTASQQLLTWEWQSGQMAVTSTFPMNVSVSALTVADLN
                     VDNHLDVLAATADGALAVLMGNSQGGFGGVQLFPAVAGTTRLEVTQMNVDGTLDVVMN
                     DSAGQLVVLPGTGDGGFGQPQVLPASGASAWETGRMNLDAMADVITLESGAIRTFVTQ
                     QTARTVTTTQDNGDNESPTAGSLRAALLAAQAGDSIVFAIPGAQVTGNI"
     gene            complement(1878..>2324)
                     /locus_tag="PKZ53_14710"
     CDS             complement(1878..>2324)
                     /locus_tag="PKZ53_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41743.1"
                     /translation="DGRFSVWFPPGFQPPKYETKALMEGGHSLPLHTVAVESDESVCL
                     VAYSDYPAQLFELMDSQTILESVLDGAMGQLNGVVERHESLVIQGMPAVSVLFTATKA
                     GQKTYGRYVYLVVRPRLYQVGFVSGNRAEIEKPAINQFFQSFQSTP"
BASE COUNT          574 a          642 c          582 g          526 t
ORIGIN      
        1 aatattacct gtcacctggg cgccaggaat cgcaaacaca atcgaatcac cggcctgggc
       61 ggccagcaac gcggcacgca gtgagcctgc cgttggggat tcattgtcac cgttgtcctg
      121 ggtggtggtc acggtgcggg cggtttgctg ggtcacaaaa gttcgtatgg cgcctgactc
      181 aagggtgatc acatccgcca tcgcatccag attcatccga ccggtttccc aggccgaggc
      241 cccactggct ggcaacactt gtggctggcc aaatccgccg tcacccgtac caggaagcac
      301 gaccagttgc ccggctgaat cattcatcac cacatccagc gtcccatcaa cattcatttg
      361 ggtcacttca aggcgggtgg ttccagccac ggcggggaat aactgaacac cgccaaagcc
      421 accttgtgag ttccccatca ggacagccag cgcgccatca gcggtggcag ccagcacatc
      481 cagatgattg tccacattca ggtcagccac agtcagagcg gagactgaaa cattcattgg
      541 aaacgttgag gtaacggcca tctgaccact ctgccattcc caggtcagca attgctggga
      601 agccgtgtca acggtccaga ccgtacttgc tgacggtgcc gtggccagat aattcaaact
      661 gactccggca ggtgcggcca tatgttgccc ggctgccaat ccatcgtttg ttcccagaca
      721 cagcaccagt ccgtcgcctg cactgaccac aacatcctgg atgtcgtcct ggttgacatc
      781 caccaccgcc agggcagtgg cattgggcaa aactggctga atggccagaa tcgaaagcgg
      841 caaccgattg gcttcgccac ggacagtctg gctctggtca gcctgccccc aaacggtgac
      901 agtgctgttg ctcgcattca acgcgagcaa ttccggatag ccatccccgt tgatatcacc
      961 cacagccagc gccgtggtgc tggaaccagt ttccagtatt tcaggcaaac caaattctgt
     1021 ggcggcctga cctcgctgca attgtaagtt tccatttttg acagcaatca gatcggctat
     1081 cccatcgccg tcaacatcgg catacctgac gactgaaggt ccagccgcaa ccgtggcaag
     1141 atctgaagcc gactccagat caaatgctgc gtactggggg agcggaacgg ccacctgacg
     1201 tgatttctgc cagacaggtt gggctgccgt agagttggcg gttacagaaa aacgactacc
     1261 tgcggcgaca gcaagtagcg ctataaacac cgataacgca ataaaattgt tccttttatt
     1321 catagaacac ccttattaaa tccccttaat ttcttcctaa taaactgatc agccgataac
     1381 acactgattt tcaacaaatt ctcaaatcga gtgaccctat caggaaggct cggtaacaaa
     1441 ccacccaccc cattttattt tctggggatg gacgcgggtt aagttcacat tctggtgaaa
     1501 cttgtgcaga acccctggta cagcacaggg ctcagtggtc gctggtctcg gtgataatgc
     1561 gcgtcagtga atatcaatac tgaaatgact gaaacccacg ctggcaaatc agtgaaccag
     1621 accctgttac agatgcctgg ctgattcatc agtggatctc atcattccaa aaatggcagg
     1681 agctcaaaaa tccgtgcctt gttttgttat tggtcgtggc ctgccaaacg cgtgtactga
     1741 aattgaatct attttttgac tgaatcgttg ggattgaatg atcgagttca attggcttgt
     1801 gaactggtct atgagcttgg ttgaatgatc tgggcgatat gttggttttc aactgccgga
     1861 agaataacga attgtgccta cggtgtagat tgaaatgact ggaaaaactg gttaatggct
     1921 ggtttttcga tttcagcacg attccccgat acaaaaccaa cctgatacag tcggggcctg
     1981 acaacaagat acacatagcg gccataggtt ttttggccag ctttggtggc ggtaaacagc
     2041 actgaaacgg cgggcatgcc ctgaataacc agcgattcgt gtcgttccac aaccccgttg
     2101 agttgcccca tcgccccatc aagcacactc tccagtattg tctgagaatc catcagttca
     2161 aagagttgag ccgggtaatc gctgtaggcc accagacaga cactctcatc tgattcaacg
     2221 gcaaccgtat gcagcggcag ggagtggccg ccttccatca aggctttggt ttcgtatttc
     2281 ggcggctgaa atccaggcgg aaaccacaca gaaaaccggc catc
//
LOCUS       DAOQDO010000954         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42111_length_2322_cov_3.143802, whole genome shotgun sequence.
ACCESSION   DAOQDO010000954 DAOQDO010000000
VERSION     DAOQDO010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42111_length_2322_cov_3.143802"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..>2322
                     /locus_tag="PKZ53_14715"
     CDS             406..>2322
                     /locus_tag="PKZ53_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41744.1"
                     /translation="MLPGLLFGVFYSSPPVYAQYCFDSWTTDNGLPQNGLRAIAQTPD
                     GYLWFTTFDGLVRFDGVRFTVFNRSNSKGIHSNRFTNLYVDQEGNLWAATEDKSLTIY
                     RHGQFTSYTQADGLPEGLGGTHIFEFKSGPQGEVLILSATGWYQLRGQEFIFVEAADP
                     NKHHKIYFGPSGTQWTVTEHVVYASKGGNLTTYPLEINLPELSPTVRLPNIQDHDNVY
                     EDRHGNLWLGDLSGLYCLRDGAIRFYGEDDGIPANTLFHSFWEEPDGSLWFATGESDF
                     SGIGLVRYKNNRFTAWGTEVGLSSDRILSVFKDREGTIWLAANRGLNRLRHQVVKPFP
                     SQSGGVHREIYPILQTHTGDIYVGSNHGLAQIRGTTFTNFPIEVAEYESPVTALWEDD
                     SQSLWVGAVDHLFHRVNGQFRLVADLPPLYTPVAIYSDPSGKVWIGTNKGLLQFQGEK
                     LIAQYTTKDGLPSNEIKVIHQDRSGTLWIGTYGGLARLVNGSFVAITETDGLTGNRVR
                     SIYEDERGTLWIGTYDSGLSRLQNGQFFNYRVENGLYDSGVFCILEDHRGWFWISCNR
                     GIYRLSRQQLNDLAEGRRAGIHCIAYNRQDGMRNTECNGGAQPAGIKTNDGKLWFPTQ
                     DGLAVIDPESVINNP"
BASE COUNT          548 a          532 c          620 g          622 t
ORIGIN      
        1 gaatgaagaa tgaagaattg agaatgaaga atgaagaatg aagaatgaag aatgtgatac
       61 gtggtgagtg atgcaggtta cctgaatttt gtccaaaata gcaccaccgt ttacgtcctt
      121 tttgtccttt gatctcatct gacactggcc tgcatccttg tggctgacat tttcgggcct
      181 caatcaatgt gcggttcttt tgtcagaacc cattgaaaag gcggcggtaa cttttccacc
      241 ggggaagtgc ttgattttgg ccttctgaat acccctggtt tcctgttccg ggtggaatct
      301 gttatttttg gcaatctttc attttgtcaa agccagtcac tgccggatat gcgaaatgta
      361 ctgttgccct ttactggttt caggctctgg agattggtct ggctcatgct tccgggcctg
      421 ctgtttgggg tattttattc atcaccccca gtgtatgctc aatattgttt cgattcgtgg
      481 acgactgaca acggattgcc tcaaaacgga ttgcgggcga ttgcgcaaac cccggacgga
      541 tatttgtggt tcacgacctt tgatgggctg gtgcgctttg acggagttcg gtttacagtc
      601 tttaaccgga gcaattccaa agggatccac agcaatcgct ttaccaattt gtacgtggat
      661 caagagggca atctgtgggc ggcgactgaa gataaatcgc tgacgatcta tcgccatggg
      721 cagtttactt cctacactca ggccgatggt ctgcctgaag gactcggcgg gacacatatt
      781 ttcgaattca aatccggccc gcagggtgaa gttttgatcc tgtctgccac gggttggtac
      841 caactgcgcg gccaggaatt cattttcgtg gaggccgcag acccaaataa acaccataaa
      901 atctattttg ggccttctgg aacccaatgg accgttaccg aacatgtggt gtatgcctca
      961 aaaggtggga atctgaccac gtatccgcta gagattaacc ttcctgaact cagcccgacg
     1021 gtccggcttc ccaacatcca ggatcacgac aatgtctatg aagaccgaca tgggaatttg
     1081 tggctgggcg atctgtcagg tttgtactgc ctgcgcgatg gtgctatcag attctatggc
     1141 gaggatgatg gtattcctgc gaacacactc tttcattcat tttgggaaga acccgacggg
     1201 agtctctggt ttgccaccgg tgagagcgat ttttcaggga ttggattagt gaggtacaaa
     1261 aataatcgct ttactgcctg gggaacggag gtcgggcttt ccagtgatcg aattttgtca
     1321 gtgtttaagg atcgagaagg caccatctgg ctggccgcca accggggact caatcgctta
     1381 cgacaccagg tcgtcaagcc tttcccatcg cagagtggag gggtccaccg tgaaatctat
     1441 ccgattctcc aaacccacac cggtgatatt tacgttggct caaatcatgg tttagctcag
     1501 attcggggca caacattcac aaattttccg attgaagtgg ctgagtatga aagtccagtc
     1561 actgctttgt gggaagacga cagccaatct ttgtgggttg gggcagttga tcatttgttc
     1621 catcgggtca atggtcagtt caggttggtg gctgatctcc cacctttgta taccccggta
     1681 gccatctact ctgacccttc tggaaaagtc tggattggca ccaataaagg attattacaa
     1741 tttcagggag agaagttgat tgctcagtac acgaccaaag atgggcttcc cagcaatgag
     1801 atcaaggtta ttcatcaaga tcgttccggt accttatgga ttggaacgta tggcgggttg
     1861 gcacgcctgg tcaatggaag ctttgtggcc atcactgaaa ctgatggatt gactggcaat
     1921 cgggtgcgct ccatttatga agatgagaga ggcacactct ggattggcac ctatgacagt
     1981 ggcttgagtc gcttgcaaaa tgggcaattt tttaactacc gggttgagaa tggactctat
     2041 gactccggtg tgttttgtat tctcgaagac caccggggct ggttttggat cagttgcaac
     2101 cgggggattt accggctgag ccgccagcaa ctcaacgacc tggctgaagg tcgccgggca
     2161 ggaattcatt gcattgccta taaccggcag gacggaatgc gcaataccga gtgcaatggc
     2221 ggggcacagc cggctgggat caagaccaat gatggcaaat tatggtttcc gacccaggat
     2281 ggcctcgccg tgattgaccc agaatccgtt atcaataacc cg
//
LOCUS       DAOQDO010000955         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42125_length_2322_cov_2.325540, whole genome shotgun sequence.
ACCESSION   DAOQDO010000955 DAOQDO010000000
VERSION     DAOQDO010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42125_length_2322_cov_2.325540"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..604
                     /locus_tag="PKZ53_14720"
     CDS             152..604
                     /locus_tag="PKZ53_14720"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNJ41745.1"
                     /translation="MSRLLPLMIKAAGPNEEILESACFAAWDLAVGQATSRISAPVSL
                     SGKLLTVATLDQRWKKQLEALTDQVLFRLNSTLGQPLVARIQVVVDSAWVEEKRRSLP
                     TLLPPQPLPPPDPDLLDVANTIEDPKLRDQYLRTATRYLNHPSRDSKR"
     gene            642..1220
                     /locus_tag="PKZ53_14725"
     CDS             642..1220
                     /locus_tag="PKZ53_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ41746.1"
                     /translation="MGISLSKGGNVSLEKSEPGLKRVLVGLGWDARGTDGADFDLDAS
                     VFMLAATGKVRSDNDFVFYNNLTSPDGSVVHSGDNRTGAGEGDDEAVRVDLTRVPGDV
                     QKMVFCVTIHDAEARRQNFGMIGSAFIRIVNDENAREIVRYDLSEDASIETAMIFGEI
                     YRHNNEWKFRAVGQGYKGGLAAMARGFGVNVG"
     gene            complement(1400..1873)
                     /locus_tag="PKZ53_14730"
     CDS             complement(1400..1873)
                     /locus_tag="PKZ53_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41747.1"
                     /translation="MGYTTTFSGVFYFNKPLTPKAIERLKKLERMDGRDFDDDRPQGY
                     CQWNLTKDLKGLTWDGAERFYNYIEWLEWIIKRILRPSKIELNGAVEYQGEYIGDCGA
                     IIVKANQVRLARVSVLEALELKAFKEFVLDSEYGEAIWERWRLHKKTLQAKVNQS"
     gene            complement(1977..>2322)
                     /locus_tag="PKZ53_14735"
     CDS             complement(1977..>2322)
                     /locus_tag="PKZ53_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011611749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41748.1"
                     /translation="EELKRFRAVLFQIEKDGPEGKRWGNSKDVIASIQGFANYVAMVN
                     PEKGAEFQAQVKAIIQKYGWQRPASHQKRPLVSESQYPGPASSTTPEPPSVTTPEPES
                     PKKDEKKWWKLF"
BASE COUNT          593 a          572 c          535 g          622 t
ORIGIN      
        1 aggaaatgat cgaagaacaa aataaacgag cgcaacagca atcagcccca gccaaccaaa
       61 aataaaacca tctctggtct ttcctgaatt acttttgtat ctgaatcaaa ctgatctgaa
      121 ctggtggggg aagaaggatg ccatggaaag tatgtcacgg ttgctgcccc tgatgatcaa
      181 ggcggcgggc ccaaatgaag aaattctgga aagcgcctgt tttgcggcct gggatctggc
      241 cgtcgggcag gcaacatccc ggattagtgc tcccgtcagc ctgtcaggaa aacttttgac
      301 cgtggccaca cttgaccagc gctggaaaaa acagcttgag gcgctcaccg atcaggttct
      361 ctttcggctt aactcaacac tgggtcagcc acttgtggcc cgcattcagg tggtcgtgga
      421 ttcggcctgg gtggaagaaa agcggcgctc tctcccgacg ctccttcccc cacaaccact
      481 tccaccacct gaccctgatt tgctcgatgt agccaacacg attgaagatc ccaaattgag
      541 ggatcaatat ctgcgaaccg cgacacgata tctcaaccat ccgtctcgcg attcgaaacg
      601 ctaactttca tttcttcaac aatttgtcat gggaggcaac aatgggaatc tctctttcca
      661 aaggtggcaa tgtttccctc gaaaaaagcg aaccagggct gaaaagagtt ctggttggtc
      721 tgggatggga tgcgcgcggc actgatggcg cggattttga cctcgatgcc agtgtcttta
      781 tgctggcagc cacaggaaaa gttcgtagcg ataacgattt tgtgttctat aacaacctca
      841 cgtcaccgga tggatcagtt gtccatagcg gtgataatcg cactggggct ggtgaaggtg
      901 acgacgaagc tgttcgggtt gatctgaccc gggttccagg tgatgttcag aaaatggtgt
      961 tctgtgtcac gattcacgac gccgaagccc ggcgccagaa ctttggcatg attggctcag
     1021 cattcattcg cattgtgaat gatgaaaacg cccgtgaaat tgtccgctat gacctttcgg
     1081 aagatgccag tattgaaaca gccatgattt ttggcgaaat ttaccgccac aacaatgaat
     1141 ggaagtttcg ggctgtcgga caggggtaca aaggtggcct ggccgctatg gctcgcggct
     1201 ttggggtcaa tgttgggtaa gcaatgcgcg agtggatcag tttgtagtca gtagtcagta
     1261 gttcgctaag tttgtttggt taaatcactt gactattttc tcatacaggg actgcaaatt
     1321 gattatgaca cctcaaaaga aaaagccgtg gtttgtaaac cacggccttt tcttttgaac
     1381 caaaaaccag ccattgagct tatgactggt tcacttttgc ctgtaacgtc tttttatgaa
     1441 ggcgccaccg ttcccaaatg gcttcgccat attcgctatc taaaacaaac tctttaaagg
     1501 ctttcagttc cagtgcttcc agaactgaaa ccctcgccag tcgtacctga ttggctttca
     1561 caatgatggc gccacaatct ccgatgtatt cgccttgata ttcaacagct ccatttaatt
     1621 caatttttga tggccttaga attcgcttaa taatccattc gagccactca atatagttat
     1681 aaaatctctc agcaccatcc caggtcaacc cctttaaatc tttggtcaaa ttccactgac
     1741 aatacccttg agggcgatca tcatcaaaat cacgtccgtc cattcgttca agctttttca
     1801 gcctctcgat ggctttaggt gtaagaggct tattaaagta aaacactcca gagaatgtgg
     1861 ttgtgtatcc catagaccag cccctcacaa acatatgaaa tcaaaccatt taatagcgaa
     1921 gataatcatt gagcttgact caatgcctgt cgtaccactt gctattcgtt tttcacttaa
     1981 aacagcttcc accacttctt ttcgtctttt ttgggtgact caggttctgg agtcgtgacg
     2041 gaaggtggtt cgggggttgt tgaagatgcc ggacctgggt actgggattc agacacaaga
     2101 ggccgctttt gatgtgaggc cgggcgttgc cacccatatt tctgaataat tgctttcacc
     2161 tgagcctgaa actcagcgcc tttttcagga ttgaccatgg ccacgtagtt ggcaaaaccc
     2221 tgaatcgaag caattacatc ctttgaattt ccccatcgct tcccttctgg accatctttt
     2281 tcaatctgaa acaacacggc ccgaaagcgc tttaattcct ca
//
LOCUS       DAOQDO010000956         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42171_length_2320_cov_4.546137, whole genome shotgun sequence.
ACCESSION   DAOQDO010000956 DAOQDO010000000
VERSION     DAOQDO010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42171_length_2320_cov_4.546137"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..584)
                     /locus_tag="PKZ53_14740"
     CDS             complement(105..584)
                     /locus_tag="PKZ53_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ41749.1"
                     /translation="MHPNEQLINKFYSSFQKRDHVGMNECYHPNAEFTDEAFVGLKGK
                     EVHAMWHMLCERAKDFDMTFSHVQADDRKGSANWEARYTFRLTGRPVHNVIKASFEFQ
                     DGKIIRHRDTFDFYKWARMAFGMKGVLLGATPFFKKAVQRKVQITLQEFMQAKKYNG"
     gene            complement(694..2076)
                     /gene="xseA"
                     /locus_tag="PKZ53_14745"
     CDS             complement(694..2076)
                     /gene="xseA"
                     /locus_tag="PKZ53_14745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNJ41750.1"
                     /translation="MSLFDLLTQSEERRPVSVSELTAQIRRRLEGEFGAVIVQGEISN
                     FKAHSSGHWYFTLKDDRAQIRGACFRNANQRIRFRPADGLSVIVRGSLSVYEPRGDYQ
                     LLATMIEPVGVGSLQLAFEQLKARLEKEGLFDPAHKRPIPLMPRRVGVVTSPTGAAVR
                     DIIRVLSRRNDTVSLLLYPAAVEGENAAKEIRAGIEYFNTHNTGDTAVDVLIVGRGGG
                     SAESLWAFNDEALARAIHQSQIPVISAVGHEVDYTIADFVADLRAPTPSAAAEMVAIQ
                     KSEIVGQLTRAEATLERHIRYKLLVARSRWQELARHQVFSDVQSRLRDTQQWLDELSQ
                     RLETSMVTGLRSLGDREQRATQQLHSFDWSRYFQLQRQRVGQTEARLQNVLAHQLEAR
                     QARLEQAAARLEMLSPLRVLRRGYALVWNQQGQLVKRAVEVKSGDLLRLRVSEGEVSC
                     TVLDSQPEES"
BASE COUNT          583 a          592 c          602 g          543 t
ORIGIN      
        1 aagcaccaag caccaagcac taaacaccaa gcaccaagca ctaaacacca agcactaaac
       61 accaagcacc aagcaccaag tactaaacac caggcacctg aatttcagcc attgtacttc
      121 ttggcctgca tgaattcttg aagcgtaatt tggacttttc gctggacggc ttttttgaaa
      181 aacggagtcg cgccgagcaa tacacctttc attccaaaag ccatccgggc ccatttgtag
      241 aaatcaaatg tgtcccgatg gcggatgatt tttccatcct ggaattcaaa tgaggctttg
      301 atgacattgt gaaccggtcg cccagtcagc cgaaatgtgt agcgggcctc ccagttggcc
      361 gatcccttgc ggtcatcagc ctgcacgtgg ctgaaggtca tatcaaagtc ttttgcccgt
      421 tcacacagca tgtgccacat cgcatggact tctttgcctt ttaagccgac aaacgcttca
      481 tcggtgaatt cagcgttggg gtgataacac tcattcattc cgacgtggtc gcgcttctgg
      541 aaacttgaat agaacttgtt gatgagttgt tcatttgggt gcataaggtt tgagaatgaa
      601 aatgggccgg aaagaaggaa acaactgtat tccttcgagt tgagacgggt tgggtggggt
      661 gtgcaagtta tgaaggtttg gagtttcgag agattatgat tcttcaggct gcgaatcaag
      721 aacagtacag gatacttcac cttcactgac tcggaggcga agaaggtctc cggatttgac
      781 ttcaaccgca cgcttgacca gttgcccctg ctgattccat accagtgcat acccacggcg
      841 caacactcgc aacggtgaga gcatttccag acgggctgct gcctgctcta accgtgcctg
      901 gcgggcttcc agttgatgag ccagcacatt ctgcaatcgg gcttcggttt gcccaacacg
      961 ctggcgttga agttgaaaat agcgtgacca gtcaaatgaa tgaagttgtt gtgttgctcg
     1021 ctgttcacgg tcgccaagtg atcgcaggcc agtcaccatg ctggtttcca gccgttgcga
     1081 caactcatca agccattgct gggtgtcgcg gagccgtgat tgaacatccg aaaagacctg
     1141 atgtcgggct aactcctgcc agcgtgaccg ggcaaccagg agtttatatc gaatgtgccg
     1201 ctccagcgtc gcttcggcac gggtcaattg cccaacgatt tcacttttct gaatcgctac
     1261 catctctgca gcggctgatg gtgtcggggc ccgcaaatcg gccacaaagt cagcaatcgt
     1321 gtaatcaact tcgtggccaa cggctgaaat aactggaatt tgagattgat ggatggctcg
     1381 cgccagggct tcgtcattaa atgcccagag tgattcagcc gagccgccgc cacgcccaac
     1441 aatcaacaca tcaacggcag tgtcgccggt gttgtgggtg ttgaaatatt cgattccagc
     1501 tcgaatttct ttggcggcgt tctctccctc aaccgctgcc ggatagagca aaaggctgac
     1561 ggtgtcattg cgacgtgaaa ggacccggat aatatcgcga actgcggcac cagtcggaga
     1621 ggtcaccaca cccacccggc gaggcatcag cggaatgggt cgtttgtgtg ccgggtcaaa
     1681 taacccttct ttttcaagcc gcgctttcaa ttgttcaaag gccagttgaa gtgaaccaac
     1741 ccccaccggc tcgatcatcg tggccagcaa ttgataatcg ccacgaggtt catagaccga
     1801 caggcttcca cgaacgatca cactcaaccc atcggctggg cgaaatcgaa tgcgctgatt
     1861 ggcattgcga aaacaggcgc cgcggatttg cgcccgatca tctttgaggg tgaaatacca
     1921 gtgcccggag gaatgggctt tgaaatttga gatttcacct tgaacgatca ccgccccaaa
     1981 ttcaccctcc aggcggcggc gaatctgggc cgttaactca gaaacagaga ccgggcgacg
     2041 ttcttcagat tgggtgagta aatcgaaaag gctcatcagg ttcagggaaa ggaggggata
     2101 tagaaaaaat tgaaactgat gatgtgagga aaatcaccgt ttcggcactc aaactatcgc
     2161 tggaattacc agtttggcaa tctttttttc gccagcaatc gcttgagtgc cgaagaattt
     2221 caggccggtt gggaaacaac tggttggatt tgttcagcaa agagcgcatg aatccgggta
     2281 tcagcggtta gttctggatg aaacgtcgcc gccagaatat
//
LOCUS       DAOQDO010000957         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42240_length_2318_cov_3.823243, whole genome shotgun sequence.
ACCESSION   DAOQDO010000957 DAOQDO010000000
VERSION     DAOQDO010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42240_length_2318_cov_3.823243"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1947
                     /locus_tag="PKZ53_14750"
     CDS             310..1947
                     /locus_tag="PKZ53_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNJ41751.1"
                     /translation="MVKPQNPILFEGHLKSNLPYDLSGGLVVFLVALPLCLGIALASG
                     APLFSGVIAGIVGGLVVGALSGSEISVSGPAAGLAVMVAGGIKSSGSFKAFLAVVVLS
                     GILQIIFGLFRGGRIGDYVPTSVIKGMLAAIGIVIILKQIPHALGQDKDYIGDFGFLQ
                     GVGNDNTLFAIVQAILTWNPEAVVIALISLAILLLWERPFLKQNRILSFVPAPLLVVV
                     IGTLINELFRLFFTGFYLRAEDGHLVSLPIATSLSTFFNQFTLPSLSSLTNPEVYTLA
                     ITFAVVGSLETLVAIEAADKIDPFKRISNTNRELMAQGIGNITSGMLGGLPITSVVIR
                     TSTNVYAGSRTRMATIFHGVLLLIAVFLIPTFLNRIPLACLAAILCVVGYKLANVSVF
                     KEMYRQGSEQFIPFIITIVAIVFTDLLVGITIGFFFGLFFVIRANHHSAMTCVDQDNY
                     YLIRFNKDMTFVNKAELKEQLTKIPNDVILILDATKSLYIDQDIYEVITEFEELAKYR
                     NITIEKKNLDSKIPPQLGWMKLFGGPNGRIQETSAGE"
     gene            1913..>2318
                     /locus_tag="PKZ53_14755"
     CDS             1913..>2318
                     /locus_tag="PKZ53_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNJ41752.1"
                     /translation="MEEYKKLLLANKAWVKEKLDMREDYFLRHKDVQTPHFLWVGCAD
                     SRVPAEDITGTEPGELFVHRNVANLVVHTDLNLLSVLQYAVDVLKVKHVIICGHYGCG
                     GVKSATSHKNLGLINKWLHSIKDVYTLHKAELN"
BASE COUNT          557 a          548 c          590 g          623 t
ORIGIN      
        1 gaagagatgg acggctggga agttgtggtc aaccggcttc cggaatatct tcctgggtgc
       61 cccgcctatg ctgactggtg gcaggttgtt gcacaccctc cttttgcccg aaatgaaacc
      121 caactctacc cggttgacaa taaagatgaa taacaaccgc ccgcacatcg agaaaagctg
      181 gtcgaattga gcaaagacct gtaagttagc ggagttttcg aaatttcgac gatatttggg
      241 gctgtgatca actgcgaaga agaccaacag cctttttgag caaatggaaa ggttgagcag
      301 aaaaaccgta tggtgaaacc acaaaatccg attttgttcg aaggtcactt gaaatcaaac
      361 ctgccctatg acctgtcagg tggactggtt gtctttctgg tggccctgcc cttatgcctt
      421 ggcattgcac tggcttctgg cgcaccgctg ttttcaggtg tgattgccgg tattgtcggt
      481 gggttggtgg ttggggcgct ctccggatcc gaaatcagcg tcagcgggcc cgccgccggt
      541 ctggcggtga tggtcgctgg cggtatcaaa tcaagcggca gtttcaaagc tttcctggcc
      601 gtagtcgttt tatcagggat cctccaaatt atttttggac tgttccgggg tggaagaatc
      661 ggagattacg tgccgacctc ggttatcaaa ggaatgcttg ccgccattgg gatcgtcatt
      721 attctcaagc aaatccccca cgcgcttgga caggataaag attacatcgg ggattttgga
      781 tttctccagg gcgtgggcaa tgacaatacg ctctttgcca tcgttcaggc gatccttacc
      841 tggaacccgg aagccgtggt tattgccttg atttcactgg cgattctatt gctctgggaa
      901 cgaccgttct tgaaacagaa tcggattcta agttttgtcc cggcaccgtt gctggtggtc
      961 gtgattggga cgctcatcaa tgaactgttt cggctgttct ttaccgggtt ttatttacgg
     1021 gccgaggatg gtcatctggt ctcgctgcca attgcaacca gcctgagcac ttttttcaat
     1081 cagttcacct tgccgagtct ctcatccttg accaatcctg aagtgtacac cctggcaatc
     1141 acctttgccg tggttggcag tcttgaaacc ctggtggcga ttgaagctgc ggacaaaatt
     1201 gatccgttca agcgcatttc aaacaccaat cgtgagttga tggcccaggg aatcggcaat
     1261 attacctctg gaatgctcgg cgggttgccg attacctcgg tggtgattcg gacctcaacc
     1321 aatgtttatg ctgggagccg aacccggatg gcgacaatct ttcacggggt actcttactg
     1381 atcgcggtgt ttctgatccc aaccttcctg aaccgcattc ccctggcctg tctggccgcg
     1441 attttatgtg ttgtcgggta caaactggcg aacgtgagtg ttttcaaaga gatgtaccgt
     1501 caggggagtg aacaattcat tccatttatc attaccatcg tggccattgt gtttacggat
     1561 ttgctggtgg ggatcaccat tgggttcttc tttgggctct tttttgtgat tcgggcaaac
     1621 catcattccg ctatgacgtg tgttgaccag gacaattact acctgattcg ctttaacaaa
     1681 gacatgacct ttgtcaacaa agccgaactt aaagaacaac tgaccaaaat tccaaatgat
     1741 gtcatcctga tccttgacgc gaccaaatcg ctctatattg accaggatat ttacgaagtg
     1801 attaccgagt ttgaagaact ggccaaatac cgcaatatca ccatcgagaa aaagaatctc
     1861 gattcaaaaa ttcccccgca gcttgggtgg atgaaacttt ttggaggacc aaatggaaga
     1921 atacaagaaa cttctgctgg cgaataaagc ctgggtgaaa gaaaagctcg acatgcggga
     1981 agattacttt ttgcggcaca aagacgtcca gacacctcac tttttgtggg ttggttgcgc
     2041 tgatagtcgt gttccggcgg aagatatcac tggaactgag cccggagaac tctttgtcca
     2101 ccgaaatgtg gccaaccttg tggttcatac ggatttgaac ttgctgagcg tccttcaata
     2161 tgcggtggat gtactcaagg tcaaacacgt catcatttgc ggacactatg ggtgcggagg
     2221 cgtaaaaagt gcaacttctc acaaaaatct gggcttgatc aacaagtggc tgcacagtat
     2281 caaagatgtc tataccctcc ataaagctga actcaatg
//
LOCUS       DAOQDO010000958         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42278_length_2317_cov_3.410698, whole genome shotgun sequence.
ACCESSION   DAOQDO010000958 DAOQDO010000000
VERSION     DAOQDO010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42278_length_2317_cov_3.410698"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..>2317)
                     /locus_tag="PKZ53_14760"
     CDS             complement(51..>2317)
                     /locus_tag="PKZ53_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ41753.1"
                     /translation="DGKNIPLTHKALETLLFLVKRHGQVVEKEELIEAIWPDTFVEEG
                     SLTRNISVLRKTLGEGPSDHHFIETVPKRGYRFVAEVREVAFSAPAPVNGSSFMGTVA
                     ELNLEASAETLVLEDTLDEDSGEISVELPETTAEILVEKESSLEVEPVSCPTPTPGPR
                     FWAVQLGMILGVMALMIAGAVVGIRYLVDSKANAHPFQLSEMKRLTNSGKTIDAAISQ
                     DGRYLVYVQDEGELQSLWIRQISTASDVQILPPSNVVYQGLDFSPDGNYVYYNLWDKV
                     GVGVVFRIPTLGGMSSRVVADVMPSLAISPDGQCVAVIRSDAVNNTKMLVIANTDGSG
                     ERVLIQRKEFEWLAWPAWSPDGKSISYVGSRLTQKDNDSRVVARVYEMPLQGGDEVVL
                     SSYPWRDVTGMKWLKDKSGLVMAATDQIGAPMQIWFVPNHGGVPQRVTNDVNGYRGLS
                     LATDSNALITVQSEVLSGIWVSPVDDYTQARQITSDRNEGIGFSWTPDDRLVHSSWRS
                     GNLDIWIMDRDGSHKQQLTSNAGVNFRPQVSPDGQTIVFGSTRKDAQHLWKMNIDGSN
                     PQQLTNEECADFACFLPKQDELLYVATKDGKAPLFRASLKTGEKKQLNATFCYLPAVS
                     PDGKYVAYSYWNERSIPQQWAREIMAIDGSEPPRPINIPKSAVGSIGAVMFQWNPNGE
                     GLLYIDTQNGVSNLWELPLNGGTPRQVTSFIEGRIQSFAWSPDRKFMAFGRSVTSSDV
                     IQIRGFSPATQEAK"
BASE COUNT          575 a          630 c          518 g          594 t
ORIGIN      
        1 ttcattcttc attcttcatt cttcattctc aattcttcat tcttcattct tcattttgcc
       61 tcttgggtcg caggggaaaa tcctctgatt tgaattacat cactgctggt gacggatctt
      121 ccaaacgcca tgaatttccg atccggcgac caggcaaaag attgaatccg gccttcgata
      181 aaactggtca cctggcgtgg cgttccgcca ttgagtggga gttcccacaa atttgagact
      241 ccattttgag tgtcaatata gagcaaacct tccccattgg gattccactg aaacataacc
      301 gccccaatag atccaaccgc agattttgga atattaatcg gacgtggtgg ttcgctcccg
      361 tcaattgcca taatttcacg ggcccattgc tgaggaatgg aacgttcgtt ccagtatgaa
      421 taagcaacgt acttaccatc aggtgaaaca gccggcagat agcagaatgt agcattaagc
      481 tgtttttttt caccggtttt gagcgatgcc cgaaacagtg gcgcttttcc atctttggtg
      541 gcgacataca agagttcatc ctgttttggt aaaaaacagg caaagtcggc acattcttca
      601 ttggtcagtt gttgtggatt tgagccatca atattcattt tccacaaatg ctgggcgtct
      661 ttacgggttg agccaaaaac aatagtttgt ccatctggag aaacctgggg gcggaaattg
      721 acgccggcat tggaggttaa ttgctgtttg tggcttccat cgcggtccat aatccagata
      781 tcaagatttc cgctccgcca ggatgaatgg acgagccggt catctggggt ccagctaaat
      841 ccaatccctt cattgcgatc cgaggtaatt tgccgggcct gggtatagtc atcaactggt
      901 gaaacccata tccctgagag aacttctgat tgaacggtaa tcaaggcatt tgagtcagtg
      961 gccagactca atccgcgata tccattgaca tcattggtca cccgctgggg cacgcctccg
     1021 tgatttggaa caaaccagat ctgcatgggg gccccaatct ggtcagtggc ggccatgacc
     1081 agcccggatt tatccttcaa ccacttcatg ccagtgacat ctctccaggg atagcttgaa
     1141 aggacaacct catctcctcc ctggagtggc atttcataga cgcgagcgac cacccggctg
     1201 tcattatctt tttgagtgag ccgggagcca acatagctaa tcgactttcc atccggtgac
     1261 caggctggcc aggccagcca ctcaaattcc ttacgctgga tcaggactcg ttccccgctc
     1321 ccatcagtat tggcaatgac cagcattttg gtattgttga cggcatcgct acggatcacc
     1381 gccacacatt gcccatctgg tgaaatcgcc agcgatggca ttacatctgc cactactctc
     1441 gatgacattc cacccaacgt cggaatgcgg aacaccacgc cgacgcctac cttatcccac
     1501 agattataat agacataatt tccatctggc gaaaagtcga gtccctgata gaccacattg
     1561 gatggaggaa gaatctggac atcgctggca gtcgaaattt gccggatcca gagcgattgc
     1621 aactcgcctt catcctgcac atacaccaga tagcgaccat cctgcgaaat tgcagcatca
     1681 atcgttttgc cactattggt caggcgtttc atttccgata gttgaaatgg gtgtgcattc
     1741 gcttttgaat caacaagata ccgtatcccc accacagccc cagcaatcat cagagccatc
     1801 acgcccagta tcatcccaag ttgcactgcc caaaaccgtg gacctggggt gggagtgggg
     1861 caagataccg gctcaacttc aagacttgac tctttttcaa ccaggatttc agccgtcgtt
     1921 tctggcaatt ccaccgaaat ttcgccactg tcctcatcaa gcgtgtcttc cagaaccagt
     1981 gtctcagccg aagcttcaag gttgagttca gccacagtcc ccataaatga cgagccattg
     2041 accggtgcgg gcgccgaaaa agcgacttct cgaacttcag ccacaaaccg atacccacgt
     2101 tttggaacgg tttcgatgaa atgatgatct gacggcccct cccccagggt cttgcgcaac
     2161 actgaaatat tgcgcgtcaa acttccttct tcaacaaacg tgtcaggcca gatagcctcg
     2221 atcaattcct ctttttcaac cacctgccca tggcgtttga ccagaaaaag aagggtctca
     2281 agtgccttgt gggtcagcgg aatattcttt ccatcac
//
LOCUS       DAOQDO010000959         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42280_length_2317_cov_3.136163, whole genome shotgun sequence.
ACCESSION   DAOQDO010000959 DAOQDO010000000
VERSION     DAOQDO010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42280_length_2317_cov_3.136163"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /gene="wzy"
                     /locus_tag="PKZ53_14765"
     CDS             <1..1044
                     /gene="wzy"
                     /locus_tag="PKZ53_14765"
                     /inference="COORDINATES: protein motif:HMM:NF025654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HNJ41754.1"
                     /translation="QRQVFPALVSSDRLAGWLGIVILGTCLAAWFGSVWVVGGSSVFT
                     GSYESFLLATSDTNLSWLYFGIGFGLVWLACTTEKRIKQVGLGAFIGWALIALPLGLR
                     GEVLFPGVTYWVLSRRSETQKSAWKVAILGLCLLSVISALRVIREVGVENVQLASLTA
                     SPSEAMVELGSSLRPVSTVVTWTENGDEFLNGASYWAPIDRALVWVIPGWGRVNAEDD
                     DRILNVQVMKRVGPIGFSPVAEAYYNFGPLGVVIAMMITGWLIGWISGFADSPLNRAL
                     LGVTFLPVLIQIRNSFVAVPFQLIVGLATVWMIYWLCGWISYRKSLVEPAVLTSDQKK
                     YKPSASNQTSRLS"
     gene            1041..2306
                     /locus_tag="PKZ53_14770"
     CDS             1041..2306
                     /locus_tag="PKZ53_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ41755.1"
                     /translation="MRVCVTLEFRFQQLADGSVWTPGSFAYPFWERYLEVFDTVQVIA
                     RVRRVSSVPSDWKRADGRQVSFAPIPDYHGPLQFLMQSPQVIRAVRQAITSDDAVILR
                     VGSQLAACVEPVLTQTGQPFGVEVVGDPYDAFSPKAIRHPLRPFFRWWFTREQQRQCR
                     NAWAVSYVTRYYLQQRYPNGIHGIQAFYSDVELTPDRFVTRETVTGRYSERRFEGDRE
                     YRLITLASLEQMYKAPDVLIDACALCLQAGYNLRLVICGDGKYRSSLEALARKREIAN
                     RVEFRGHVAGFAAVAGELDQSDVFVLPSRTEGLPRALIEAMARGLPCIGSNVGGIPEL
                     LPEHCLVPAGDTLALAHRISEFLDQPEVMLSAALRNLDQAHEYEEAKLHHARLEYYRA
                     VRETTEAWVAAKVFLKKNSLKSDQVFEIQ"
BASE COUNT          470 a          561 c          661 g          625 t
ORIGIN      
        1 cagcgccagg ttttccctgc gctggtatcg agcgaccggt tagccggctg gttgggcatt
       61 gtcattttag gaacctgtct ggccgcctgg tttggcagcg tgtgggtggt tgggggaagc
      121 agcgttttta ccggttccta tgaatccttc ttgctggcga caagcgacac caatctgtcc
      181 tggctgtatt tcggcattgg atttggactg gtctggctgg cctgcaccac tgagaaaagg
      241 ataaagcagg ttggcctggg cgcttttatt ggctgggcac tgattgccct gccgttgggc
      301 cttcggggcg aagttctgtt ccctggtgtg acctattggg tgctctcgcg ccgttcagaa
      361 acccaaaaat cagcctggaa agttgcgatt ttaggactgt gtctgctgag tgttatttcg
      421 gctctgcggg tgatccgcga agttggggtt gaaaacgttc aactcgcttc actgaccgcc
      481 agcccatctg aagcaatggt agagcttgga agttcattgc ggcctgtttc gaccgtggtg
      541 acctggactg aaaacgggga tgaatttctc aatggtgcca gttattgggc acccattgat
      601 cgggctctgg tctgggtcat tcctgggtgg ggccgggtga atgccgaaga tgatgaccgg
      661 attctcaatg tgcaggtgat gaaacgagtg ggcccgattg ggttctcacc cgtagctgaa
      721 gcctattaca attttggccc gcttggggtg gtgattgcca tgatgataac cggctggctg
      781 attggatgga tttccggatt tgccgattcg cccttaaatc gagccttgct gggggtgacg
      841 ttccttccgg tattaatcca gattcgcaat agttttgttg cggtcccgtt tcaactcatt
      901 gtcggtttgg ccaccgtgtg gatgatttac tggctgtgtg gctggatcag ttaccgaaaa
      961 tccctggtcg agcccgccgt cctcacttcc gaccagaaaa aatataaacc gtccgcttcc
     1021 aatcaaacat cgaggttgtc atgagagtct gtgtcacgct tgagtttcga tttcagcaac
     1081 tggccgatgg ttcggtatgg acgcctggtt cgtttgccta tccattttgg gagcgctacc
     1141 ttgaagtttt tgacactgtt caggtgattg ctcgggtcag gcgggtctcg tcagttcctt
     1201 cagattggaa gcgtgctgat ggcaggcagg tcagctttgc gccgattcca gactatcacg
     1261 gaccgctcca gtttttgatg cagtcccccc aggtcatccg ggcggttcgt caggcgatta
     1321 cttccgacga tgctgtgatt ttgcgtgttg gttctcaact ggcagcctgt gtcgaaccag
     1381 ttttgactca aactggccaa cctttcggag tcgaagtcgt gggcgaccca tatgatgcct
     1441 tttcccccaa agcgattcgc cacccattgc ggccattttt tcgatggtgg tttacccgag
     1501 aacaacaacg ccagtgccgc aatgcctggg cggtcagtta tgtgaccaga tactatcttc
     1561 agcagcgata tccgaacggg atccacggaa tccaggcatt ttattcggac gttgaactca
     1621 cccccgaccg gtttgtgacc agggaaaccg tcactggtcg ctattcagaa cgcaggtttg
     1681 aaggagatcg tgaatacagg cttattacac tggccagcct tgagcaaatg tacaaagcgc
     1741 cagatgtgtt aattgacgcc tgtgcattgt gtctgcaggc cgggtacaac ctccgactgg
     1801 tgatttgcgg tgatgggaaa taccgttcca gccttgaagc gctggctcgc aaacgtgaaa
     1861 ttgccaaccg ggttgaattt cgtggccatg tggctgggtt tgctgccgtg gccggtgaac
     1921 ttgatcagtc agatgtattt gtccttccgt cacgaactga agggttacca cgagcactga
     1981 ttgaagccat ggcccgtgga ttgccctgta ttgggtcaaa tgtcggtgga atccctgaat
     2041 tgttacctga gcattgtttg gttccggcag gtgatacact ggcactcgcc catcgcatta
     2101 gtgaattttt ggatcagcca gaggtgatgt tgagcgcggc cctgcgaaat ctggatcagg
     2161 cccacgagta tgaagaagcc aaactccatc acgcccggtt ggagtattac agagccgttc
     2221 gtgaaaccac cgaagcctgg gttgcggcta aggtttttct gaaaaaaaac agcctgaaat
     2281 cagatcaagt ctttgaaata caataatatc agtcagt
//
LOCUS       DAOQDO010000960         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42281_length_2317_cov_3.110080, whole genome shotgun sequence.
ACCESSION   DAOQDO010000960 DAOQDO010000000
VERSION     DAOQDO010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42281_length_2317_cov_3.110080"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PKZ53_14775"
     CDS             <1..1026
                     /locus_tag="PKZ53_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ41756.1"
                     /translation="EGGEYFVALKIPLLRGARINEKAIAERQARLGEPLADVNFAQNR
                     LDLLLKASVSYWDWVAAKRKMDVARNLLDIARFRATAIQERADLGDLPPIDKVEAEQE
                     VRRREGNLTKAERDLQKAAFKLSLFLWQDNGLPAPLPVADQVPVMTPPPVVPAIDVIT
                     RARTEAIDRRPELKGLQIQRDITQLDLDLAQNQRLPAVDFTVSPGRDVGDQGIGSTIK
                     AGVSITLPLRQRTADGRAAASRLKIQKLELETRSERLRILTEVDDAVSALQTAEQRYQ
                     AAQQELQLAITLERGERDRFALGDSTLFLVNQRERATAEAATKVIEVQAEFEQAKAAF
                     RAAAGQF"
     gene            1231..1695
                     /locus_tag="PKZ53_14780"
     CDS             1231..1695
                     /locus_tag="PKZ53_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41757.1"
                     /translation="MSHPHLALSLARQTIELIDQWPWKPVSQEWERKKIEKMQPLLHK
                     ALARLDHPAEAGSAVIVCRISVVTSKADEYEEWFERSRVWEVICAADSVQCQSHSNLA
                     TMVGASQSDWFVVCATDRETPEMLKQAEVWRSKLAQAIVQPEVYVDVVERKP"
     gene            complement(1767..>2317)
                     /locus_tag="PKZ53_14785"
     CDS             complement(1767..>2317)
                     /locus_tag="PKZ53_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNJ41758.1"
                     /translation="VANTACVYEAFLKKFKKTALKPVTLKPLELVNHVSGCKVHNRDA
                     NELIEEVECDILYLDPPYNERQYSSNYHILETIARYDNPLIAGVTGIRKGYARSSYCR
                     KREVIQAFEHLVRQAKTRHILLSYNDEGLMTFDEIHRILGTRGEVKTFQTTYNRFKAD
                     NGRKYKRSRTVEYVHYVRVTKS"
BASE COUNT          556 a          591 c          640 g          530 t
ORIGIN      
        1 gaaggcgggg agtactttgt ggcgttgaaa attccgctgt tgcgtggggc aaggatcaat
       61 gaaaaagcaa ttgctgagcg tcaggcccgg cttggcgaac cactggccga tgtcaatttt
      121 gcccagaacc ggctggattt gttgctcaaa gcgtcggtaa gttactggga ttgggtcgcg
      181 gccaaacgca aaatggatgt tgcccgcaac ttgctcgaca ttgcccggtt tcgggcgact
      241 gcgatccagg agcgggctga tttgggtgac ttgcccccga ttgacaaagt tgaagctgaa
      301 caagaggtgc gccgtcgtga aggaaatttg accaaagccg agcgcgactt acagaaagcg
      361 gccttcaaat tgtcgctctt cctctggcag gacaacggat tacctgctcc attgcccgta
      421 gctgaccagg ttccagtcat gacaccgccg cccgtggtac cagcaataga tgtcattacc
      481 cgtgcccgga ccgaagccat tgaccgccgt ccagagttaa aagggcttca gattcagcgc
      541 gacattaccc agcttgatct ggatctggcc cagaatcagc gactgccagc ggttgacttt
      601 acggtaagtc caggtcggga tgtcggagat caaggcattg gctcaaccat taaagcgggt
      661 gtctcaatta ccctgccgct ccggcaacga acggctgatg gacgtgcggc agccagtcgg
      721 ttaaaaattc agaaactgga gcttgaaacc cgaagcgaac gcctgcgaat tctgaccgaa
      781 gtggatgatg ccgtctcagc cctgcaaacc gccgaacaac gctatcaggc tgcccagcag
      841 gaattacaac tggcgattac gctcgaacgg ggcgaacgcg accggtttgc gcttggtgac
      901 agcactctct ttctggtcaa tcagcgcgaa cgtgccaccg ccgaagctgc caccaaagtg
      961 atcgaagtcc aggccgaatt tgaacaggcc aaagcggcgt tccgtgccgc tgcgggacaa
     1021 ttttaaaata ccgggctggc tgggggctga agaagtcggg ctgaagaata tcgggctgaa
     1081 gtctcgcaag ctcggggctg aagaatatcg ggctgaagtc tcgcaagctc ggggctgaag
     1141 aagtcgggct cagggtatta aaacccgact tcttcagccc ccagccctca gcccaggttt
     1201 ttcagccccc aacccccaac ccccaatcgt atgtcccatc ctcatcttgc gctctccctt
     1261 gcccgacaaa ccatagaact cattgaccag tggccgtgga agcctgtttc tcaggaatgg
     1321 gaacgcaaaa aaattgaaaa aatgcagcca ctgctccaca aagcgctggc tcgtctcgat
     1381 catccagcgg aggctggatc cgccgtgatt gtctgccgga tttcagtggt gacgtcgaaa
     1441 gctgacgaat atgaagaatg gtttgagcgg tcgcgggttt gggaagtgat ttgcgccgct
     1501 gattcggttc aatgccagtc acacagcaac ctggctacca tggtcggggc aagccagagt
     1561 gactggtttg tcgtctgtgc aactgatcgc gaaacaccag agatgctcaa acaggcagaa
     1621 gtgtggcgaa gcaaactggc ccaggccata gtccaaccag aggtgtatgt ggatgtggtt
     1681 gagcggaagc cgtgaagatt cagggatggg gatttgacca gatcgaagtt tgttctttca
     1741 acggggagtg attgttgcgg tgacgctcac gatttcgtca cccgaacata gtgaacatac
     1801 tcaacggttc gactgcgttt atacttgcgg ccattgtcag ctttaaagcg gttgtatgta
     1861 gtctggaagg tcttgacctc gcctcgcgtg cctaaaatcc gatggatttc atcaaacgtc
     1921 atcaatcctt catcattgta actcaagaga atatgccgtg tttttgcctg ccgcaccagg
     1981 tgttcaaaag cctggatgac ttcgcgtttt cgacaatagg aactacgggc atatcctttt
     2041 cggatgccag tcacgccagc aatcagaggg ttatcgtagc gggcaatggt ttccaggata
     2101 tggtaatttg acgagtactg gcgctcattg tagggtggat ccagatataa aatgtcgcat
     2161 tcaacctctt caatcaattc attggcatca cggttgtgga ctttgcatcc tgaaacatga
     2221 tttaccagct ctaaaggctt gagcgttact ggtttcaagg ctgttttttt gaatttcttc
     2281 agaaaagctt catagacaca ggcggtgttg gccaccc
//
LOCUS       DAOQDO010000961         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42353_length_2315_cov_2.911062, whole genome shotgun sequence.
ACCESSION   DAOQDO010000961 DAOQDO010000000
VERSION     DAOQDO010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42353_length_2315_cov_2.911062"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..2123
                     /locus_tag="PKZ53_14790"
     CDS             237..2123
                     /locus_tag="PKZ53_14790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ41759.1"
                     /translation="MSHRVREHHQQLLKSEIGTLRKPLGLGLNIALAYANTYYIAMSN
                     LGFQAVYKLFNLYETVTCERAFLPDRDILEDMERARLPLVTLESQTPVNEFDVLAFSI
                     SFETDYLNVPTMLRLAGIPVLREDRSESDPLVVVGGAATFLNPEPIADFVDVVCVGEG
                     EGLVPQLVEFIEQSSSRAELLERLSYEPGFYVPSFYDVEYNDDGTIADIVPKGRAPRR
                     VLRVRAAMRKQESKIKDAPEYVNFVPESLILTPHTEMSNRFMMEISRGCSMGCRFCWA
                     GFSYLPPRVFAAENLLDRASRARPHTDEIGLVATAVCDHPEITTLLGGLRDMEYRISV
                     SSLRLDQITDELLDALVESCDQTIAVAPETGSYRLRRAINKNLTDEEILDICQRIFNR
                     GILNLKLYMMVGLPTEEQEDIDAIVSLTRRVRQLMIDEGRRFGRVGRVVVSLNGFVPK
                     PHTPLQWEPMIPEKQLDKKIQYLTRELPKIKNVEVRAMSSRIAYQQTMLSLGDRRLSQ
                     FLIENDRLRNFKQALRMTDLDIDFYTLRRKGVDEVLASDMIDNGLSREFLLKEYNRAV
                     ASRTTPPCPAADACTRCAVCDEKDGAISYAVNQLVQLGRMTPAVQSSFGSLNTIAAAA
                     KLRV"
BASE COUNT          585 a          587 c          605 g          538 t
ORIGIN      
        1 aatttagcca ggctaagtgt tgaaaacagg ttttatttta gcgttttgag atagtactct
       61 tagtacttgg agcttggtgc ttagtgctta ggaatcaata ctttcgaaga accaagcacc
      121 aagaaccaag caccaagaaa ccaagaaacc aagcaccaag aaccaagaac caagcaccaa
      181 gcaccaagaa ccaagaacca agcaccaagc accaagaacc aagaacccaa gtctttatgt
      241 ctcatcgagt tcgggaacat caccagcagt tactcaaaag tgaaatcggc accctgcgca
      301 agccacttgg cctggggcta aacatcgcgc tggcctatgc caacacctat tatatcgcca
      361 tgtcaaacct tgggtttcag gcggtttaca agctctttaa cctctatgaa acggtcactt
      421 gcgaacgtgc ctttctgccg gatcgtgata ttttggagga catggagcgt gcacgcctgc
      481 cgctggtgac gcttgaatcc cagaccccgg tcaacgaatt tgacgtcctt gccttttcaa
      541 tctcctttga aaccgattat ctcaatgtgc caaccatgct tcggctggct ggaatcccgg
      601 ttttgcgtga agaccgaagc gaaagtgacc cgctcgttgt ggtcggcggc gcggctacgt
      661 tcttgaatcc agagccgatt gctgattttg tggatgtggt ctgtgtcgga gaaggcgagg
      721 ggctggttcc gcaactggtt gaatttattg agcagtcttc gtcacgagcc gagttgctcg
      781 aacgattgag ctatgaacca gggttttatg tgccgtcttt ttatgatgtc gaatacaacg
      841 atgatggcac gattgcagac attgttccca aaggacgggc gccgcggcgg gtcctccggg
      901 ttcgagcggc gatgcgcaaa caggaatcaa aaattaagga cgcgccagaa tacgtcaatt
      961 tcgttcctga atcgctcatc ctgacgccgc acaccgaaat gtccaaccgg tttatgatgg
     1021 aaatttcgcg tggctgctca atgggctgcc ggttctgctg ggcagggttt agttatttgc
     1081 cgccgcgtgt gtttgcggct gaaaatctgc tcgaccgcgc atcacgtgcc cgcccgcata
     1141 cagacgaaat tggcctggtc gccaccgccg tatgtgacca tccagaaatc acgacgttgc
     1201 tgggtggctt gcgtgacatg gagtaccgaa tttcggtttc cagccttcgc ctggaccaga
     1261 ttactgatga attgcttgat gctctggttg aaagttgcga ccagacaatt gcggtggccc
     1321 cggaaaccgg gtcctatcgc ctgcgccggg ccattaacaa gaatctgacc gatgaggaaa
     1381 tcctggatat ctgtcagcgc attttcaatc gcggtatcct gaacctgaaa ctctatatga
     1441 tggttgggct tccgaccgaa gagcaggaag atatcgacgc aattgtgagt ctcacccggc
     1501 gtgtccggca gttaatgatt gatgaaggcc gtcggtttgg tcgggttggc cgggtggtgg
     1561 tgtcgctcaa cgggtttgtg ccaaagccgc acaccccgct ccagtgggag ccgatgattc
     1621 ctgaaaaaca actggacaag aaaatccagt acctgacgcg tgaacttccc aaaatcaaaa
     1681 atgtagaagt gcgtgccatg tcatctcgga ttgcctatca acagaccatg ctcagtcttg
     1741 gagatcggcg actctcacaa tttctgatcg aaaacgaccg gctgcgaaac tttaaacagg
     1801 cgctgcgcat gacggacctg gatattgatt tctacacctt gcgccgcaag ggagtggatg
     1861 aagtcctggc ctcagatatg attgacaacg gcctgagccg tgaattcttg ctcaaggaat
     1921 acaatcgggc ggtggcttcg cggaccacgc caccctgtcc tgcggctgat gcctgcaccc
     1981 gatgtgctgt gtgcgatgaa aaagacggtg ctatcagtta tgctgtcaac cagttggtgc
     2041 aacttgggcg gatgaccccg gcggtgcaat catcatttgg gtcactcaat acgattgccg
     2101 ctgctgccaa actgcgggtt taaaaccagc gggaaagcaa ggacataaag gacataaggg
     2161 acaaaaatct ctgttgaatg gaataaaatt tcaggaaaaa acaccaaaac cccgcactat
     2221 caaaagctga tagtgcgggg ttttggtttt catctcaatt tcggaaaccc ggaacccgga
     2281 acccggaacc cggaaccctg attttaaaaa gtcac
//
LOCUS       DAOQDO010000962         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42358_length_2315_cov_2.815044, whole genome shotgun sequence.
ACCESSION   DAOQDO010000962 DAOQDO010000000
VERSION     DAOQDO010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42358_length_2315_cov_2.815044"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="PKZ53_14795"
     CDS             complement(<1..2224)
                     /locus_tag="PKZ53_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41760.1"
                     /translation="MLSLEPTFQENWAGMVSWLEIQAQESTSELAAIWQDVKELLTRQ
                     ILEAVVPEFPEAISSDLNTYRDFRQHTATTLSEAVVAALDRKRLFKRIIKLIETFDRN
                     VDEALKQLPETPVISEKQISDMFETGVNSTLARLWLKLRLRQRKLNWQVVVTEEWQTL
                     NQERLKREGQLLLELALLFRKLQTPWDLVRTRLDEQVKGKRGTPVDSGAKLELQKQIV
                     LRRIHHFETALTRHKLFSERLRSKLEARLSSRLAVRSLSPQSKKSPPGPEFLKLWVEQ
                     LRAVEAEARLEIELTCQESNLIVLFSQYLDQIGRERNALRTELQMMTQEVRQQVESQV
                     PVTLPAIETDIVPAPSRVHDLESAFRKQLRPLQVPVELVAWLSPYPPKKPKPKLLRPS
                     DFYLRTFDRRVRNGVEQLFADLEAQHTAMVTQVEQAREVIVFGTEALDAHLENDRTVA
                     REAFHNALRMLERLNQSDTSWRLPVERRLVELLAGMFLEVRVLLSRDYLGALAYLAQK
                     GTSSAFSEKVEAGSEVMTGLIQKSVDVGETGFTEFLVAIGWKQRFDPGKSEIYRRTYL
                     PKEFTADLSKKEMPAIYRRLFRFEAVDDPRFLIGRAEELAAIAEARTNWEAGRQVAVL
                     ITGPRGSGKTSLINCAIKRHLAGVEVIRGDFSERITSIPHLHAHLARLFGLDSADDLE
                     QFLLEHRRIVILEEIERLFLRQIGHYLAVREIQRLIAATCSTTFWILATNQVAFHFLD
                     P"
BASE COUNT          527 a          608 c          624 g          556 t
ORIGIN      
        1 ctggatccag aaaatgaaag gccacctgat tggtcgccag aatccaaaac gtagtcgagc
       61 aggtcgcggc aatcaggcgc tgaatctcac gaacggccag ataatgccca atctggcgca
      121 ggaaaaggcg ttcgatttct tccaaaatga cgattcggcg atgttccaac agaaattgtt
      181 ccaggtcgtc agcagaatcc aatccaaaca ggcgagccag atgagcgtgc aggtgcggga
      241 tcgaggtgat gcgctcgcta aaatcacccc ggataacctc gactccggcc aaatgccgtt
      301 tgatggcaca attgatcagg ctggttttgc cgctgccgcg aggaccggtg atcaaaacag
      361 ccacctggcg gccagcttcc cagttggttc gagcttcggc aatcgcagcc agttcttcag
      421 cccggccaat caaaaagcgt ggatcgtcaa ccgcttcaaa ccgaaacaac cgccggtaaa
      481 tagccggcat ttcttttttg gaaagatcgg cggtgaattc ttttggaagg taggtccggc
      541 ggtaaatttc agatttgcca gggtcaaagc gttgtttcca gccgatagcc accagaaatt
      601 cagtaaagcc ggtttccccg acatccactg atttttggat cagcccggtc atgacctccg
      661 agccggcctc gaccttttca ctgaaggctg aactggtgcc tttttgagcc agataggcca
      721 gtgcccccag ataatcgcgg gaaagcaaga cccgaacttc cagaaacatc ccggccagaa
      781 gctcgaccaa ccggcgttcg accgggagtc gccaactggt gtcagactgg ttgaggcgtt
      841 ccagcatccg cagcgcattg tggaaggctt cgcgggcgac ggtccgatca ttttccagat
      901 gagcatcgag cgcctccgtg ccaaacacga tgacttcgcg tgcctgttcg acctgggtca
      961 ccattgcggt gtgctgggct tccagatcgg caaagagttg ttccacgccg ttgcgtaccc
     1021 ggcggtcaaa cgtgcggagg taaaaatcag atggccgcag gagctttggt ttgggttttt
     1081 tgggcggata ggggctcaac caggccacaa gttcaaccgg cacctggagc ggcctgagtt
     1141 gtttgcgaaa ggccgactct aaatcgtgaa cccgactggg cgccgggacg atgtcggttt
     1201 ctatggcggg gagagtgacc ggtacctggg attccacctg ctggcgaacc tcctgagtca
     1261 tcatttggag ttcggtgcgc agagcgtttc gttcgcgacc aatctggtcg agatactggc
     1321 taaagagcac gatcaggttg ctttcctggc aggtcaattc gatctcaagc cgcgcctccg
     1381 cttcgaccgc ccggagttgt tcaacccaca gttttaaaaa ttccggccct ggcggcgatt
     1441 tttttgattg tggagacaga gaccgtacgg ccaatcggct cgatagtcgg gcttcaagtt
     1501 ttgagcgcaa gcgttcggaa aacaatttgt gccgcgtcaa cgccgtttca aagtgatgga
     1561 ttcgccgcaa tacgatttgt ttctggagtt cgagtttggc accagaatca accggggttc
     1621 cccgtttgcc ttttacctgc tcatccagac gggtccgaac cagatcccag ggcgtttgca
     1681 attttcgaaa cagcagtgcc agttcgagca gtaattgacc ttcccgcttg agtcgctctt
     1741 gattcagggt ttgccactct tcggtcacga ccacttgcca gttgagtttt ctctgtcgga
     1801 gtcggagttt caaccagagt cgagccagtg tcgagttgac tccggtttca aacatgtcac
     1861 tgatctgttt ttctgaaatg accggagttt caggtagctg tttgagggct tcgtcaacgt
     1921 tgcggtcaaa agtttcaatc agtttgatga ttcgtttgaa taatcgtttt cgatctaggg
     1981 ctgcgacaac tgcttcactc agggtcgtgg ccgtgtgttg acgaaaatcg cgataggtgt
     2041 tgaggtccga gctgattgcc tctggaaatt ccggaaccac cgcttccagt atctgccggg
     2101 tcaacaattc tttgacatcc tgccagattg cagccagttc actggttgat tcctgggctt
     2161 gtatttccag ccaggaaacc attccagccc agttctcttg gaacgttggt tcaagcgaaa
     2221 gcattgacat agttccagtt cgtagtcagt aagtaccttg ccgaaaattt ccagacattt
     2281 tgaaccacga aacacacgaa atacacgaaa aaaac
//
LOCUS       DAOQDO010000963         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42416_length_2313_cov_3.473871, whole genome shotgun sequence.
ACCESSION   DAOQDO010000963 DAOQDO010000000
VERSION     DAOQDO010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42416_length_2313_cov_3.473871"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1725)
                     /locus_tag="PKZ53_14800"
     CDS             complement(64..1725)
                     /locus_tag="PKZ53_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ41761.1"
                     /translation="MSSKFLFIGLDGSTFDVLDPLMAEGRMPRLKALIEQGARAPLET
                     TIPPITPTAWVSMSTGTNPGKHSIFEFLLRRGDKLDVPINSTLRDSPAVWDILSDLGH
                     QVTVTNMPVTYPPKPLNGRMVADFLTPLGKTDYTYPAELREELESRFGPYRLYITEVY
                     TRGRVDQVLEQLYDELEYKTSVNCYLMKNKPWDFYFTHIWSTDRCQHELWHIMDPTHP
                     MSDPKEVKKYREKVLKFWEKVDESVGKMVDAAGPGVTVMMGSDHGFGPITKFLCFNVW
                     MIEQGLIQLKPGAMTALKKLFFKLGLTPETGYRVSMAIGLAKLRLSMGVTNRSSLQKL
                     INKVMLSLADVDWSRTKAFSKGNYGQIFINLKGREPHGSVAPGAEYEKVCQEVITQLR
                     NLKHPETGEPLIGPIWRAEEIYSGPHVAEAPDISFLPADMRYKPLGTLDLTSNRFITD
                     VYGNSGDHRMNGFFVANGPHIKPGTRLSANIMDIAPTLLYLFNKPIPNDMDGRILKEM
                     ISDPYLASNQPVYTNQDYTGAEIASFSEEDDAEVRERLRSLGYLG"
     gene            complement(1963..>2313)
                     /locus_tag="PKZ53_14805"
     CDS             complement(1963..>2313)
                     /locus_tag="PKZ53_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ41762.1"
                     /translation="RSTPRQFTDGAIDKLLHYSWPGNVRELRATVERLVIMTQREWIE
                     PDDLPFTQTDGPLLASFRFDSYREAQEAYEREFIQRKLAENNGNVTHTAEALNMDRSH
                     LYRRIKTLGISIRG"
BASE COUNT          556 a          568 c          614 g          575 t
ORIGIN      
        1 tttgaagttc gtgagttgtt cttgaacctc gcaaccctga accccgaacc ctgaaccctc
       61 agattatcca agatagccaa ggcttcgcag gcgctcacga acttccgcat catcttcttc
      121 tgaaaaggaa gcgatttccg ctccggtgta atcctggttg gtatagaccg gctgatttga
      181 agccagatac gggtcggaaa tcatttcttt caggatgcgg ccatccatgt cgttggggat
      241 tggtttgtta aagagataga gcaaggtagg tgcaatatcc atgatattgg ccgaaagccg
      301 tgtgccaggt ttgatgtgtg gaccattggc gacaaaaaag ccattcatcc gatggtcgcc
      361 ggaattgcca tagacatcag tgataaaacg atttgaggtt aagtcaagcg ttccaagcgg
      421 tttatagcgc atatccgctg gcaggaagga aatgtcggga gcttcggcaa catgcggacc
      481 agagtagatt tcttcggctc tccaaatcgg gccaattaag ggttcaccgg tttccggatg
      541 tttcagattg cgtagttggg ttatcacttc ctggcagact ttttcatatt cggcgccagg
      601 tgccaccgaa ccgtgtggct cacgcccctt gaggttgatg aaaatctggc cgtagttgcc
      661 cttcgaaaag gctttggttc gtgaccagtc aacatccgcc agcgacaaca tgactttgtt
      721 gatcagcttt tgaagcgacg aacggttggt cactcccatc gaaagccgca atttggccag
      781 tccaatcgcc attgaaaccc gatagccggt ttcaggtgtc aatccaagct taaagaacaa
      841 ttttttgagg gcggtcatcg caccaggctt cagttgaatc aggccctgtt cgatcatcca
      901 gacgttaaag cacaaaaact tggtgattgg tccaaagcca tgatctgaac ccatcattac
      961 ggtgacccca gggcccgccg catcaaccat cttgccgacg ctttcgtcaa ctttttccca
     1021 gaacttgagc accttttcgc gatacttttt gacttccttt ggatctgaca ttgggtgcgt
     1081 cggatccata atatgccaga gttcgtgctg gcagcggtcg gtgctccaga tgtgggtaaa
     1141 gtaaaaatcc cacggcttgt ttttcattaa gtagcagttg acgctggttt tatattccag
     1201 ttcgtcatag agctgctcta gcacctgatc cacgcggcca cgggtgtaga cttcggtgat
     1261 atagagccga taagggccaa atcgggattc gagttcttcg cgcaactccg ccgggtaggt
     1321 gtaatcggtc ttgccaagcg gcgtcagaaa atcagccacc atcctgccat tgagcggttt
     1381 gggcgggtat gtcaccggca tattggtcac cgtgacctga tgacccagat ccgagaggat
     1441 atcccacaca gccggactat cgcgcaacgt cgaattgatc ggcacgtcca gtttgtcgcc
     1501 ccgccggagc aaaaactcaa aaatgctgtg tttaccagga ttggtgcccg ttgacattga
     1561 cacccaggcc gtgggtgtaa ttggtggaat ggtggtttca agtggggcgc gggcaccctg
     1621 ttcgatcagg gctttgagac gcggcatgcg tccttcggcc atcagcgggt caagaacatc
     1681 aaacgttgag ccgtcgagcc caatgaacaa aaacttacta ctcatgaaga cctaaaccaa
     1741 gaatgaagaa tgaagaattg agaatgaaga acgaagaatg aagaatgaaa taaagcatcc
     1801 gattgaacca caaccggtta gctctctcat aattcataat tcataattca taattccaga
     1861 ttttgggatg ggcggcaaag taccattcac cccagagggt gtcaaggaga gggcgggaac
     1921 gccctgattg gtgacaactg tcagttgtca ttttgttcga tgtcatccgc gaatcgaaat
     1981 tcccaacgtc ttaatccggc gatacaggtg gctgcggtcc atatttaagg cttcggcggt
     2041 gtgggtgacg ttgccgttgt tttcggccag tttgcgctgg atgaactcgc gttcgtaggc
     2101 ttcctgggct tcgcggtatg agtcaaatcg aaagcttgcc agcaacgggc cgtcagtttg
     2161 ggtaaagggc aggtcgtcgg gttcaatcca ttcgcgctgg gtcataatca ccagccgttc
     2221 gaccgtggcc cgcagttcgc ggacattccc tggccacgaa taatgaagca gcttgtcaat
     2281 cgcaccatcg gtaaactggc gtggggtgct gcg
//
LOCUS       DAOQDO010000964         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42418_length_2313_cov_3.171833, whole genome shotgun sequence.
ACCESSION   DAOQDO010000964 DAOQDO010000000
VERSION     DAOQDO010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42418_length_2313_cov_3.171833"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..670
                     /locus_tag="PKZ53_14810"
     CDS             179..670
                     /locus_tag="PKZ53_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41763.1"
                     /translation="MLFLIGFIFSVQIMVILVDEFYFHYRREIPRWERIGHPLDTLTV
                     LACYGYALSFEYSPQTVGPFIGMALFSTIFVTKDEFVHAKYCDPGEHWLHAVLFAFHP
                     LVLLAIAALWTGVSARNPLGLPTVVAGVPLKIMLIAQCLLTGVFGIYQTVYWNWICRN
                     RPA"
     gene            649..1401
                     /gene="ubiG"
                     /locus_tag="PKZ53_14815"
     CDS             649..1401
                     /gene="ubiG"
                     /locus_tag="PKZ53_14815"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNJ41764.1"
                     /translation="MSQPPRVTVNNEIYHQLGERWYTAQDDPVALLRAEAALRNPWIV
                     EQIRKQLGTGELSVLDIGCGGGFLTNLLARSGFRVVGLDQSVESLAVARLYDTTGTVQ
                     YDQGDAYRLPYPDQSFDVVCSMDFLEHVDDPKQVVAQAARVIKPGGMFFFSTFNRNPL
                     SWLVVIKGVEWFVKNTPPHMHVYHLFIKPSELARFCLDCGFANPTFYGLHPNINRAFF
                     QMLVTRVVPPDFSFTFSNSLQTGYLGIAIKRL"
     gene            1627..>2313
                     /locus_tag="PKZ53_14820"
     CDS             1627..>2313
                     /locus_tag="PKZ53_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41765.1"
                     /translation="MASLTWEDKLLQRAVDQGLVPASVFLEPITVTIPAANLNRDTSF
                     RYGPKIDPLIDQGMISEAQVQSLLREMKTEGTFDREQEMGSRWPTHFLETISDDVNPT
                     LEPKTLVDVKNFPPIDDVFPVENWDRYDFLRQLGEGGMGCVYLGWDKQLSRTVALKFI
                     RSGSRQLEERFKLEARAQARIEHENICKVFEVGEVDGQTYIAMQFITGKSLHDAHQEM
                     TLSQKVFVIKT"
BASE COUNT          542 a          563 c          580 g          628 t
ORIGIN      
        1 gcctcgcttt tggtccgggc ctgacgatgt gcggcagtgt gatggaaaaa caggccgcca
       61 ggtcagtacc acctgcgtga gcgggtgggt cacgacacat cctatcagct cacaaggtaa
      121 aatcgacaat agcttgacaa atctacccac ccgctcacgc aggtggtact gacaaaagat
      181 gctttttctg attgggttta ttttttcagt tcaaatcatg gtgattttgg tggatgagtt
      241 ttatttccac taccggcgtg aaatcccgcg ctgggaacgg attgggcatc cgcttgatac
      301 attgaccgtt ctggcctgtt atgggtacgc actttcattt gagtacagcc cgcaaacggt
      361 tggcccattt atcgggatgg cccttttttc gaccatcttt gtgaccaagg atgagtttgt
      421 ccatgccaaa tattgcgacc caggtgaaca ctggctccat gcggtattgt ttgcctttca
      481 tcccctggtt ttgctggcaa ttgcagcgct atggacaggt gtcagtgcca ggaatccact
      541 tggattgcca accgttgtcg caggtgttcc gctgaaaatt atgctcattg cccagtgttt
      601 gctgactggt gtttttggaa tctatcagac ggtttattgg aactggatat gtcgcaaccg
      661 ccccgcgtaa cagtcaacaa cgagatttac catcaacttg gcgaacgctg gtatacggct
      721 caggacgatc cagtggcact gctgcgggcc gaagcggcat tacgcaaccc ctggatcgtt
      781 gaacaaatcc ggaaacagct tggaacaggc gagttatcgg tgcttgatat tggctgtggc
      841 ggcggttttt tgaccaatct gctggcacgg tccgggtttc gggtggtggg ccttgatcaa
      901 tcggttgaaa gcctggctgt tgcccgactc tatgacacaa caggaacagt tcagtacgat
      961 caaggcgatg cttaccggct gccatatccg gatcaatcat ttgacgtcgt ttgttcgatg
     1021 gactttcttg aacatgttga tgatcctaaa caggttgttg cccaggccgc acgcgtcatc
     1081 aaacccggtg gaatgttttt tttctcaacg tttaatcgaa atccgctgag ctggctagtg
     1141 gtgatcaaag gcgttgaatg gtttgtcaaa aacaccccac ctcacatgca cgtctatcat
     1201 ctttttataa aaccctctga actggcccga ttttgcctgg attgcgggtt tgccaatccg
     1261 actttttatg ggttgcaccc aaatatcaac cgggcgtttt ttcaaatgct ggtcacacgc
     1321 gtcgtccccc ccgatttttc cttcactttt tcaaactcat tgcaaaccgg gtatttaggc
     1381 attgcaataa aacgcctttg atttgggttt caggcataag cagtctgcct cgaatcctaa
     1441 aattcggttt gaaccatcac tcaaatccaa attcaattct gcctgaaccc ccctcggata
     1501 tatgaaactg ggtcttttca acctctgatc ggatggcacc tgattaaata gtgagccctc
     1561 tggtcgagcg cagaattggt gtggaacccc aggcaggctg cacaatttcc cctggaggtc
     1621 attcccatgg catcgctcac ctgggaagat aagctgttgc agcgcgctgt tgatcagggc
     1681 ctggtgccag cctcggtatt tcttgaacca attacggtta ccattccggc tgccaatctg
     1741 aatcgagata cttcttttcg atatggacca aagattgacc ctttaattga ccagggaatg
     1801 atttcagaag cccaggtgca atccctgctc cgggaaatga agactgaggg aacgtttgat
     1861 cgggaacaag agatgggttc tcggtggccg acccactttc tggaaaccat ttcggacgat
     1921 gtgaacccca cacttgagcc aaaaacgctg gttgacgtta aaaactttcc gccgattgat
     1981 gatgtcttcc cagttgaaaa ttgggaccgg tatgactttt tgcgccagtt gggtgaaggc
     2041 gggatggggt gtgtctatct gggctgggat aaacagctta gccgaacagt tgctctaaaa
     2101 tttatccgca gcggaagccg tcagctcgaa gaacgtttta aactcgaagc ccgtgcccag
     2161 gcacgtattg aacatgaaaa tatttgcaaa gtttttgaag tcggcgaagt tgatggtcaa
     2221 acctacattg cgatgcagtt tattaccggg aagtccctgc atgacgccca ccaggaaatg
     2281 accctgagcc agaaagtgtt tgtgattaaa acc
//
LOCUS       DAOQDO010000965         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42419_length_2313_cov_3.083702, whole genome shotgun sequence.
ACCESSION   DAOQDO010000965 DAOQDO010000000
VERSION     DAOQDO010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42419_length_2313_cov_3.083702"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKZ53_14825"
     CDS             <1..315
                     /locus_tag="PKZ53_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNJ41766.1"
                     /translation="TIRSEGQFNTVVYEENDRYRNQPTRDVILLNQQDMRDLGIQTGD
                     RVTIRSEVGEMTQIRAVAFDISRGSAAMYYPESNVLVKTSVDSKSGTPAYKNTLVTVA
                     KA"
     gene            599..>2313
                     /locus_tag="PKZ53_14830"
     CDS             599..>2313
                     /locus_tag="PKZ53_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNJ41767.1"
                     /translation="MLRTVFLCTVVLTSLLAIVMPARSVRSSSLPAQPVATAPTASVD
                     PHPGLKTPAPRQDPDEEYEQLPVFTVTSANDRGAGTLREAFAKIQEDGVSRRIVFHLE
                     PSDPNYDPQTGVWRVKAKIPFVLTKDMIFIDGLSQTEYGGDTNSSGPEVVIDGSDVKP
                     QIVDVGETSVYVTSLFTVFGGSKNWIRGLDIVIPRNLDFFSGAISAIILTKSSPTAAP
                     ITKNRITDCYIGINPDGETIPVGNWNGIILEAGVQETLIDNNVIVSPGIDISITGQGE
                     EELDSLKTVKTTIRNNKIGTNSSGTKRLTPFIRGPVNSALPPPTIYIKDRASETLIER
                     NIISGTRSGGVGPINQFINATSLFNTIRENRIGIGPNGENIAPSRGEDRGAYAIGIFA
                     IPGDFVSKNVIGFFPFVGIYVRNHKGLDATQQTRILENQIINIPVGMQIAQVDNVQIS
                     KNSFTRCSRGGIAVCAKVFTAADVFDIPPINPFGLVTTRVNITQNNFVRVQGPGIGFM
                     VSPVDLFIGNYSPNLLTGPALGPNSYQPSPIIESATLRPDGSIEVKGKVTVAADGTIE
                     VFASG"
BASE COUNT          588 a          585 c          586 g          554 t
ORIGIN      
        1 accattcggt ctgaaggaca gttcaatacc gtggtttatg aagaaaacga ccgctatcgc
       61 aaccaaccaa cccgggatgt cattttgctg aatcagcagg acatgcgcga tttgggaatt
      121 caaactggcg accgggtgac aatccggtct gaagtcggag aaatgaccca gattcgagcc
      181 gttgcttttg acatcagccg gggctcagcg gcgatgtatt atccggaatc caatgtgctg
      241 gtgaaaacga gtgtggattc aaagagtggc accccggcct acaaaaacac actggtcacc
      301 gtggccaaag cctagtattg tgtgggggaa cttcactccc agttcaaatc tccccaccaa
      361 tatctggtgt tcgatttttg caaatacttc aatttcaaat aattaccaaa atcaaagagc
      421 tatcctcaag ctcggtcagc tcaatctggt gaccacctga ttgcccgttg gattgctgca
      481 acgagcaact gaggccacca gttggaggcg aggtgagcgt cagggcctga atacgccagt
      541 gcggttctgg ccaatctgca acccgaccct ctgattgaag aaagaagtag gaaatggaat
      601 gttgagaaca gtttttcttt gcaccgttgt tttgaccagc ctgctggcca tcgtgatgcc
      661 agccaggtca gtccgttcat cttcactccc ggcacaaccc gttgcgacgg cacccaccgc
      721 ttcggttgat cctcaccctg gactcaaaac cccagcgccg cgacaggatc cagatgaaga
      781 atatgagcaa ctcccggtat ttacggtgac cagtgccaat gatcgtgggg cgggaacgct
      841 acgcgaagcc tttgccaaaa tccaggaaga tggcgtcagc cggcggattg tgtttcattt
      901 ggagccgtct gacccaaatt atgacccaca aaccggtgtg tggcgggtca aggccaagat
      961 tccttttgtg ttgaccaagg acatgatctt tattgatgga ctgtcccaaa cggaatatgg
     1021 aggcgatacc aactccagcg ggcccgaagt tgtgattgat ggctcggatg tgaaacctca
     1081 aattgtggac gttggtgaga cgagcgtgta tgtgacatct ctgtttacgg tcttcggagg
     1141 aagcaaaaac tggattcgcg ggctcgatat tgtgattccg cgcaatttag acttctttag
     1201 cggtgccatc agcgccatca ttcttaccaa atcttcgcca accgcggcgc caatcacaaa
     1261 aaaccggatt accgattgtt atattgggat caatcccgac ggcgagacca tcccggtcgg
     1321 gaactggaat gggattatcc ttgaagccgg cgtgcaggaa accttgattg ataacaacgt
     1381 gattgtcagt cctgggattg atatttcgat cacgggtcaa ggtgaggaag aacttgattc
     1441 cctcaagacc gtcaaaacca ccattcgaaa caataaaatc ggcaccaatt ccagtgggac
     1501 aaagcggttg acgcccttta tcagaggacc ggtcaattcg gcgcttccgc caccaacgat
     1561 ctatattaaa gatcgtgcca gcgaaacgtt gattgagcgc aacattatct ctgggacgcg
     1621 gtcgggtggg gttgggccca tcaatcagtt tatcaatgcc acgtcgctct ttaacacaat
     1681 tcgggagaat cgaatcggaa ttggacccaa tggcgaaaac attgccccca gccggggcga
     1741 agaccggggg gcctatgcca ttgggatttt tgccattcca ggtgatttcg tttcaaaaaa
     1801 cgtcatcggc ttttttcctt ttgtcggcat ctatgtgcgt aatcacaaag gacttgacgc
     1861 cactcagcag actcgtattc tggaaaacca gattattaac attccagttg ggatgcagat
     1921 cgcccaggtt gacaatgtcc agatctcgaa aaacagcttt acccgatgta gtcgaggtgg
     1981 cattgccgta tgtgccaaag tatttacggc agccgacgtg tttgacattc cgcctatcaa
     2041 cccatttggt ctggtgacga cacgggtcaa tatcacccaa aacaacttcg ttcgggtaca
     2101 ggggccagga attgggttta tggtttcacc ggttgacttg tttattggga actattcgcc
     2161 caatctgtta accggacctg ccctgggccc aaattcctat caaccatcgc ccatcattga
     2221 atctgcgacc ctgcgccctg atggctcaat cgaagtcaaa ggcaaagtaa cggttgccgc
     2281 cgatggaacc atcgaagttt ttgcgtctgg aag
//
LOCUS       DAOQDO010000966         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42515_length_2310_cov_3.788914, whole genome shotgun sequence.
ACCESSION   DAOQDO010000966 DAOQDO010000000
VERSION     DAOQDO010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42515_length_2310_cov_3.788914"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2310
                     /locus_tag="PKZ53_14835"
     CDS             <1..>2310
                     /locus_tag="PKZ53_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41768.1"
                     /translation="MILLDTNQLYVATPMGLIVYDTQTVKEINRLTRVENDPSSLSEN
                     AVTRLVLDDNQQLWVGTFAQGLNCLDLKTNRVIRYQSDPRNLTTLAGNKVTALFRDRT
                     GVIWVGDLQYGLSKYSPTSRKFHLLKYDPFGTPSLSNGFVRGIQEDQSGNIWIATQGG
                     GVNYYNPVTREIKVFSSLKNNADSLALDNVWAVFESADHRIWVGTYGRGVKLLDPKTG
                     NFTQFPLVPPRAVVTAITQTSDGNIWFGSEFGIYIFHPQDGTIQKLDSLRVYDAKWAA
                     EVQTIFEDHHGTIWIAFATGLTRLNPTPHQAIGYEEVPFQPELFQSKMVTYIAEDTDG
                     SLWFATKGYGVYGFDASGQLTTHLSEENGLSHNNAYGVIIDSAHTLWISTDDGINHYF
                     PATRKIRVYTIEDGLQGREFNRKAVFVNPKGTIFFGGINGLNWFHPDEIQDNLTVPPV
                     LITEVQIGGASQPPQTLTYLPATLTLPYHQNSFTFHFLALDFHAPERNQYAYQLTNFN
                     DTWVLAGTKREAVYTNLNPGEYIFKVRASNSDGIWNETGASLRIIVLQPLWTRWWAYA
                     FYLTAGISLLFGLVRLRVQQLKHQNEILELKVRHRTAELARSELEIKRKAEELARLVE
                     QLQTSEQVAHQAKEEAIEASRAKSTFLANMSHEIRTPLNGIIGMLDLLLNTRLSADQH
                     EFAETAHHSGQTLLTIISDILDFSKIEAKKLDLEKVSFELPPLVEEVLELLAPQADRK
                     HLNLISFVSPDVPACVTGDPSRLRQILTNL"
BASE COUNT          608 a          617 c          525 g          560 t
ORIGIN      
        1 atgatcctgc ttgataccaa ccagttgtac gttgccaccc cgatggggtt gattgtgtat
       61 gacacccaaa cagtcaaaga gatcaaccga ttgaccagag ttgaaaacga tccctccagt
      121 ttaagtgaaa acgcggtgac ccggctcgtc ctggacgaca atcaacaact gtgggttggg
      181 acttttgccc aggggctcaa ctgcctggat ctcaaaacca accgggtcat ccgctatcag
      241 agtgacccac gaaacctgac caccctggct ggaaacaaag tcacagctct ttttcgggat
      301 cgaaccgggg tgatctgggt gggtgatctt cagtatggat tgagtaaata ttccccgacg
      361 agccgtaaat tccatctgct caaatatgat ccatttggaa cgccgtcact cagtaatggg
      421 tttgtacgtg gcatccagga agaccagtct ggcaatatct ggattgccac tcaaggcgga
      481 ggcgtcaatt attacaatcc tgtgacccgc gaaatcaagg tattttccag tctcaaaaac
      541 aatgcagatt cactggcact cgataatgtc tgggcagttt ttgaaagtgc tgatcaccga
      601 atttgggttg gaacttatgg ccggggagtc aaactccttg accccaagac cggcaacttt
      661 acccagtttc cactggttcc accgagggca gtcgtgacgg caattaccca aaccagtgac
      721 ggaaatatct ggtttggctc tgaatttggt atctacattt ttcatcctca ggatggaacc
      781 attcaaaaac tggattcgct ccgcgtctat gacgccaaat gggccgccga agtccaaact
      841 atctttgagg atcatcacgg caccatctgg atcgcctttg ccaccggcct gacccgactc
      901 aacccaacac cacatcaagc cattgggtac gaagaagtcc cgtttcaacc tgagttgttt
      961 caatcaaaaa tggtgaccta tattgccgag gatacagatg gctcactctg gtttgcgacc
     1021 aaaggctatg gcgtttatgg gtttgatgcc tcaggccagc ttaccacgca tctgtctgag
     1081 gaaaacgggc tgtctcataa taatgcctat ggtgtcatta tagactcagc ccatacactc
     1141 tggatcagta cagacgatgg aatcaatcat tactttccag ctacccgcaa aattcgagtc
     1201 tatacaattg aggatggctt acagggtcgc gaatttaacc ggaaggccgt ttttgtcaac
     1261 cccaaaggta caattttctt cggtgggatc aacggattga actggtttca ccctgatgaa
     1321 atccaggata atctgacagt ccccccagtc ctgattactg aagtccaaat cggcggggca
     1381 tcacagccac cccaaacctt gacctacctt cctgcaaccc tgacgctccc ctatcatcag
     1441 aactcgttta cttttcactt tttagccctt gattttcatg cgccagagcg caatcaatat
     1501 gcctatcaac tcactaattt taacgacacc tgggtcctgg ccgggaccaa acgagaggcc
     1561 gtttatacca atttgaatcc aggtgagtac atttttaagg tcagagcttc aaattcagac
     1621 ggaatctgga acgaaaccgg tgcatcgctg cgcattattg tcctgcaacc cctctggaca
     1681 cgctggtggg catacgcctt ttatctcaca gcagggataa gtttgttgtt tggcctggtt
     1741 cggctccgcg tgcaacaact caaacatcag aatgaaattc tggaactcaa ggttcgccac
     1801 cggacggcag agctggcccg gtccgagctt gaaatcaaac gcaaagctga agaactcgcc
     1861 cggctggttg aacagttgca aacctcagag caggtcgccc accaggccaa agaagaagcc
     1921 attgaggcca gccgtgccaa aagcacattt ctggccaaca tgagccacga aatccggacc
     1981 ccgctcaatg gtatcatcgg catgctcgac ttgttgctca atacccgtct aagtgccgat
     2041 caacacgaat ttgccgaaac tgcccaccac tcggggcaaa cactgctcac catcattagt
     2101 gatattctgg atttttcaaa gattgaagcc aaaaaacttg acctggaaaa ggtgagtttt
     2161 gaattgccac cactggttga agaagtattg gaactgttag ctccacaggc agatcgaaaa
     2221 cacctcaatc tgatttcgtt tgtctcaccc gatgttccag cctgtgtcac cggggatcca
     2281 tcccgcctgc ggcagatttt gaccaacctg
//
LOCUS       DAOQDO010000967         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42566_length_2309_cov_2.634871, whole genome shotgun sequence.
ACCESSION   DAOQDO010000967 DAOQDO010000000
VERSION     DAOQDO010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42566_length_2309_cov_2.634871"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..>2309
                     /locus_tag="PKZ53_14840"
     CDS             218..>2309
                     /locus_tag="PKZ53_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41769.1"
                     /translation="MVLLMLTGGYVPCLPCWPIKEIKLQGKIDGFDTDDLIVFVEKNN
                     SGERRKLLGQVKHTIGITQGNRIFSEVIQAAWSDFTNQQLFQKGRDAIALLTGPLNAT
                     DSHNVQWLLNHAKHTKSVDEFFRNVAQAKFSPPQSGEKLKVFQHHLKAANKNIDVSPD
                     ELYAFLNHFHWLGYDLGCETGVVLSLLHSHISQFNQQYPEWAWSRVVDIVQTWNQDAG
                     TIIREKLPEDLRNAFKQPVIVPIPKELVTAQLEIVKRDWNQSRYATDLALTNLAGAWD
                     EKNQADVVILSQLLREEYSAWVPTARDILHLPDSPFSLKNGLWKITDRVDLWEAVGSR
                     IFDQTLDTFKTIVVAVLTEPNPAFDLPPDERFAASIHGKILAHSPALRQGLSEGLALL
                     GCKPNLLVNCSIRKAETTAITTIREIFTDANWVLWASLDKLLPTLAEAAPDEFLKAVE
                     NALRLTPCPFDELFAQESAGITGTNYLSGLLWALEGLAWDEKHFVRVCIILGELANHD
                     PGGKWANRPENSLTNILLPWLPQTLAPFEKRKVAVQTLCKEWPRIGWKLVIQLLPSDH
                     QVSMGSHKPSWRNPIPDNWEKGVTKGEYWQQVSYYAEFAILLAGQNPVRLAELIDHFA
                     NLPKPAFDKLIKVLSSDAIGNLPEDQRLVLWGHLTRFSSRHRKYSDTKWALGDELLSP
                     IEAVAVKIAPSNPVN"
BASE COUNT          628 a          520 c          555 g          606 t
ORIGIN      
        1 ccggcgaatg tgaatgtggt caaactggaa atgcaggagc cccgcaactg tagttttgat
       61 gcctgaatat tgagaaatgt ggatgactca ataatccaac cttttgagga atatgaagaa
      121 gaaatcacat accctaaccc tccaaagcaa gtttctcact aacgcctttt cctcgggggg
      181 aggaggaggg cactttgagg cccatgttca ggcatcattg gtactgctga tgcttactgg
      241 aggatacgtc ccctgtttac cttgctggcc gattaaggag attaaactgc aaggaaaaat
      301 tgacggtttt gataccgacg acctgatcgt gttcgttgaa aagaataact caggggaacg
      361 gcgaaaactg ctggggcagg taaaacatac gattggaatt actcagggaa atcgtatttt
      421 ttcggaagtt attcaggctg cctggagtga tttcacgaat cagcaactct ttcaaaaagg
      481 gagagacgct attgcgttgc tgacaggacc gttaaacgca acagactctc acaatgtgca
      541 gtggctatta aaccatgcca aacataccaa aagtgtcgac gagttttttc gcaatgttgc
      601 ccaggcaaaa tttagcccgc ctcaaagcgg ggaaaaattg aaggtttttc aacaccattt
      661 gaaagcagcc aataaaaata ttgatgtttc accagatgaa ctttacgcct ttctcaatca
      721 ttttcattgg ttggggtatg acttaggatg tgagacggga gttgttttat cgctcctgca
      781 ctcccatatc tcacagttca atcagcagta ccccgagtgg gcctggtctc gtgtagtgga
      841 catcgtgcaa acctggaatc aagacgcagg gaccatcatc cgagaaaaat tgcctgaaga
      901 cttaagaaat gccttcaaac agcctgtcat tgttccaatc cccaaagaac tggtaaccgc
      961 tcaattggag attgtaaaaa gagattggaa tcagtctcga tatgctactg atcttgcact
     1021 gacgaatttg gctggcgcct gggatgaaaa aaatcaagca gatgttgtga ttcttagcca
     1081 actgctgaga gaggaatatt ctgcctgggt gccaactgcc agagatatat tgcatcttcc
     1141 cgatagtcca ttttcactga aaaatggtct ttggaaaatt accgaccggg tggatctatg
     1201 ggaagcagtt gggtcacgaa tttttgatca gactctggat accttcaaaa ccattgttgt
     1261 cgctgtactg acggagccaa atcctgcttt cgatctacct ccagacgaac gatttgcggc
     1321 aagcatccat ggaaaaatcc ttgcccactc ccctgcgctt agacaaggct tgtcggaggg
     1381 actagccctt cttggctgca agcccaattt gcttgtcaac tgctcaataa gaaaagccga
     1441 gactactgct ataacaacca tccgcgagat tttcactgat gctaattggg tgttgtgggc
     1501 tagcctggac aagttattgc ctactttggc tgaggcggca cccgacgagt ttttgaaagc
     1561 tgttgagaat gccctccgat taacaccttg tccatttgac gaactttttg ctcaggagag
     1621 tgctgggatt actggaacaa attatctatc gggcttgctt tgggctttgg aggggttagc
     1681 ctgggatgaa aaacattttg tccgggtttg catcatcctt ggagagcttg ccaatcatga
     1741 tccaggaggg aaatgggcga atcgacctga gaattccttg accaacattt tgttaccctg
     1801 gcttccccaa acccttgccc cttttgaaaa acggaaggtt gcagtacaaa ccctgtgcaa
     1861 agagtggccc aggatcggtt ggaagctggt cattcaattg ctgcctagtg accaccaagt
     1921 gtcgatgggc tcccataagc catcgtggag gaaccctatt cctgacaact gggaaaaagg
     1981 ggtaaccaaa ggggaatatt ggcaacaggt ctcttattac gcagagtttg ccattttatt
     2041 agccggtcaa aatccagttc ggctggccga attgattgac catttcgcta accttcctaa
     2101 gccagctttt gataagttga tcaaagtcct ttcttcggat gctattggaa atcttccgga
     2161 agaccagcga ttggtgctgt ggggtcacct aacaaggttt tcatcacgcc atcgaaaata
     2221 ctctgacacg aaatgggctt tgggtgatga gctgctctca ccaattgaag cagttgccgt
     2281 aaaaattgcc ccttcaaacc cggtgaacc
//
LOCUS       DAOQDO010000968         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42597_length_2308_cov_3.062583, whole genome shotgun sequence.
ACCESSION   DAOQDO010000968 DAOQDO010000000
VERSION     DAOQDO010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42597_length_2308_cov_3.062583"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKZ53_14845"
     CDS             complement(<1..427)
                     /locus_tag="PKZ53_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ41770.1"
                     /translation="MTTPITQAERDFAIAHLHRTGTQLVEAVSGLSEAQLSFKPTPES
                     WTIAQITEHIVAAEVGVLQLAVGQVLKSPPGPDELREKVQGKTDLVLKAVLDRGTKVQ
                     APDRLVPQGRFATINQTIKAFQQARQQTIAFVEQTQEDLH"
     gene            complement(511..2154)
                     /locus_tag="PKZ53_14850"
     CDS             complement(511..2154)
                     /locus_tag="PKZ53_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ41771.1"
                     /translation="MLSPDTLLQNRYRILRLLGKGGMGAVYQAQDERLGCTVAVKQAF
                     FIEEELQRAFLREARLLANLRHHGLPKVIDHFMENDGQFLVMEFIPGKDLHQMLKERN
                     TPFPVAQVLDWADQLLDVLEFLHSQNPPIIHRDIKPANIKLTYRGEIILLDFGLAKGL
                     ASSNSSQTSVSVVGYSAGYAPVEQIQLTGTDQRTDLYALGATLFHLMTGSAPPDALTA
                     RAVNLAIGNRDPLRGTHELNPQVPVSVATVLAQALAFSRDQRLSSAVEMRQKLRAAGM
                     HITQSPARGTSVINLDDAPTVITPRLNQPPPETKLQTQLPFSGELTVTGQPIKNKLGM
                     EFLMIPAGEFLMGSPVSDYEKPQHLVKISKPFYLGKFPVTQKEWKALMKTNPSRYQDE
                     LLPVTNVSWDDAQLFIQKLNQCQDGVYRLPTEAEWEYACRAGTTAEYAGELKEMGWFK
                     ENTENRPHPVGLKKPNAFGLFDMHGNIWEWCSDWYGSNYYEKSPTIDPPGPGTGSFRI
                     NRGGSWMSVAIQCRSAFRGMNAPGTQLDYLGFRLVRLIS"
BASE COUNT          518 a          572 c          626 g          592 t
ORIGIN      
        1 catgcaggtc ttcttgtgtt tgttcgacaa aggcaatggt ttgctggcgg gcctgctgaa
       61 aggctttgat ggtctggtta atggttgcaa accgcccttg tggcaccagg cgatctggtg
      121 cctgaacttt tgttccacgg tctaacacgg ctttgagtac caggtcagtt ttgccctgga
      181 ctttttctcg taactcgtct ggcccaggcg gagatttgag cacctgaccg actgccagtt
      241 ggaggactcc gacttcagcc gcgacaatgt gttctgtgat ctgggcaatc gtccagcttt
      301 caggtgttgg tttaaaagaa agttgggctt cggagagacc ggaaacggct tcaaccagtt
      361 gggtgccagt tcggtgcaaa tgggcaattg cgaaatcgcg ctcagcttga gtgatgggtg
      421 tggtcatagt ttggtcaaat gtacaatgta agatggttca gaatgaatgg ctaaattgtc
      481 agggcaagga ttggtccaac cccagattgt tcaagaaatc aggcgcacca gacgaaatcc
      541 caggtaatca agctgggttc ccggtgcatt catgccacga aaggcagatc ggcattgaat
      601 cgcaactgac atccagcttc cgccgcggtt gatgcggaac gagccggtac caggtcctgg
      661 cgggtcaatg gttgggcttt tttcatagta gtttgatcca taccagtcag agcaccactc
      721 ccaaatattg ccgtgcatat caaaaagacc aaatgcattt ggttttttga gcccgaccgg
      781 gtgtggacga ttctcggtat tttctttaaa ccagcccatt tctttcaatt cgccggcata
      841 ctcggcagtg gttccagccc ggcaggcata ttcccattcg gcctcggttg ggagccgata
      901 cactccatcc tggcactggt tcaatttttg aataaaaagc tgggcatcat cccacgaaac
      961 attggtgacg ggtaacaact catcctgata gcggctgggg ttcgttttca tcagggcttt
     1021 ccattccttt tgggttacag gaaatttgcc cagataaaac ggctttgaaa ttttgaccag
     1081 gtgctgaggc ttctcataat ctgacacagg tgaacccatc aagaattccc cggctggaat
     1141 catcaaaaac tccatcccga gtttattttt aattggctgg ccagtaaccg tgagttcacc
     1201 agagaacggc aactgggttt gcagtttggt ttccggagga ggctgattga gtcgcggcgt
     1261 gatcaccgtc ggggcatcgt ccagattgat gaccgacgtg ccacgggcag gggactgggt
     1321 gatgtgcatt ccagccgcgc gtaatttttg ccgcatttcg actgccgatg acaggcgttg
     1381 gtcccggcta aaggccaggg cctgggccag gactgttgct acactgaccg gaacctgggg
     1441 gttgagttca tgcgttcccc gcaacggatc ccggttccca atcgccagat tgacggcccg
     1501 tgcggtcaac gcatccggcg gtgccgaacc tgtcatcagg tgaaacaggg ttgcccccag
     1561 cgcatacagg tcggttcgtt gatcagtacc agtcaattga atttgttcaa ccggagcata
     1621 cccggcacta tacccaacca cactgactga cgtctgtgat gaatttgaag atgccagccc
     1681 tttggccagc ccaaaatcaa gcaaaataat ttcgccgcga taggtcaatt tgatgttggc
     1741 gggcttgata tcgcgatgga tgatgggtgg attctgactg tgcaggaatt cgagcacatc
     1801 cagcagttga tcggcccaat ccaggacctg ggccaccgga aacggtgtgt tccgctcttt
     1861 gagcatctgg tgcaggtctt tgcctggaat aaattccatg accagaaatt gcccgtcatt
     1921 ctccataaaa tgatcaatga ctttgggcag tccatggtgg cgtagattgg ccagcagccg
     1981 ggcttcgcgc aagaacgcac gctgaagttc ctcttcaata aagaacgcct gtttcacggc
     2041 aaccgtgcat ccaaggcgtt catcctgtgc ctgataaacc gcccccatgc cacctttgcc
     2101 aagcaaacgc agaatgcgat accggttttg caacagtgtg tctggactca gcatgcgttg
     2161 attgttcccc agtcaggagt tacggatgaa gaatgtgctt ggtgcttggt acttggtact
     2221 tggtacttgg tacttggtgc ttggtacttg gtgcttggta cttggtactt ggtgcttagt
     2281 cgccaatact ttccaagaac gaagaacc
//
LOCUS       DAOQDO010000969         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42631_length_2307_cov_3.060391, whole genome shotgun sequence.
ACCESSION   DAOQDO010000969 DAOQDO010000000
VERSION     DAOQDO010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42631_length_2307_cov_3.060391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKZ53_14855"
     CDS             complement(<1..218)
                     /locus_tag="PKZ53_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ41772.1"
                     /translation="MSSVKPTADIDKYLDLLRDKDKQALAGGGAERIEKLHTAGKMTA
                     RERILAFVDEGSFEEFDRFKKHRCTDFD"
     gene            complement(703..1098)
                     /gene="msrB"
                     /locus_tag="PKZ53_14860"
                     /pseudo
     CDS             complement(703..1098)
                     /gene="msrB"
                     /locus_tag="PKZ53_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
     gene            complement(1254..2021)
                     /locus_tag="PKZ53_14865"
     CDS             complement(1254..2021)
                     /locus_tag="PKZ53_14865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNJ41773.1"
                     /translation="MFRLDGQIVLITGGATGIGRAMAEVFCEAGAKLAIASRRMAEGE
                     EIVRLIQQIGGEAIHIGADITLESDVKRVVQATLAHYNQIDVLVNNAGIMRRGAVTEL
                     TEEDWDEVLETDLKGVFLCAKHVLPPMLDRQYGAIVNIAAIHGLKGGAGTTAAYTAAK
                     GGLVSLSKSMAVRYGPQGVRVNCICPGFVPTDMNRHLIEEAPDPAERRREYEAGYPLR
                     RLGTPHDVAYAALFLASSEASWITGATLVVDGGLIAK"
BASE COUNT          571 a          572 c          551 g          613 t
ORIGIN      
        1 atatcaaaat cagtgcagcg gtgtttttta aagcgatcaa attcttcaaa ggaaccttca
       61 tccacaaacg ccaggatgcg ttcacgggcc gtcattttcc ctgccgtatg gagtttttcg
      121 attcgttcgg caccgccgcc agccagcgcc tgtttatctt tgtcacggag aagatcaagg
      181 tatttatcaa tatctgctgt tggtttcaca gatgacatcg gggaaagtcc tcactcaatg
      241 gtagtttggt cggggcaaca cagtcagtac cacctgcgtg agcgggtggg gcgacattca
      301 ctagacatta ttgaggttaa gaattcacca ggaattgaat tccacgctgc aacccacccg
      361 ctcacgcagg tggtactgac ttgacaccag ttcttcttcg ttcctggtaa gttatctccg
      421 ataatgtcta tttctttact tttggctcgg ttggccacgg agcacgttct cgtcatagtc
      481 gcaaatcaac cgttctgggt cacgagcgag ccgggcttct tcattgagcc agattttcag
      541 gcggccattt tcagacaggc acacaatctg gtcggcatac acatagttac cagtcagttg
      601 gccatattcc tgaaaaataa accagggaat caggtggcga atatctttga attcatgaaa
      661 gaccactccg ttttctgatg ggcggggcgg tttggctggg atttcttttt tctcgaaggt
      721 caatgccacc gagttaatac agtatcgcag attcgtcggt gccgggccat cagggaagac
      781 atggcccagg tgggcatcac attgactgca caggacttcg gtgcgaggca tcagccagct
      841 ttcatctgtt tcattttgga cattgtctgg tgaaaccggc gaccagaaac tgggccaccc
      901 ggtaccggaa tcaaacttgg tttcagagct aaacagttcc tgaccacaac aggcacagcg
      961 gtaaatccct gcttctttgg tgtcataata tttgccagtg aacggacgct cggttccttt
     1021 ctgacgacaa accgcaaatt gttctggtgt cagggtttgt ttccactcag tgtcactttt
     1081 gacaactttt tctttcatga tctgtctttc aatcaattgg aagaatatga aatgttaggg
     1141 gcagtggctt atcccatttt gcaatgaaac gttcgggttg ctgaaagtct aaattattga
     1201 gaaagctgta tttccctgaa ccctgaaccc gataccctga accctaagca gtattatttg
     1261 gcaatcaatc caccatcaac cacgagtgtg gcacccgtaa tccaactggc ttcagatgag
     1321 gccaaaaaga gcgcggcata ggccacatca tgcggcgttc caagccggcg caacgggtag
     1381 ccagcctcgt actcacggcg ccgttccgcc gggtctggtg cctcctcaat cagatggcga
     1441 ttcatatcag ttgggacaaa tccagggcaa atgcaattga cccgtactcc ctgcggacca
     1501 tagcgaaccg ccattgattt gctcaggctg accaggccac ctttggctgc tgtatacgca
     1561 gccgttgttc cggcgccacc tttcaacccg tggatggccg caatgttgac gatggcgcca
     1621 tattggcggt caagcattgg cggcagaacg tgtttggcgc acaaaaacac acctttaaga
     1681 tcggtttcaa ggacttcatc ccaatcttct tcggtgagtt cagtcaccgc gccgcgccgc
     1741 ataattcctg cattattaac gagcacatca atttgattgt agtgagccag tgtcgcctga
     1801 actactcgtt tgacgtctga ttcaagggtg atgtctgcgc caatatgaat ggcttcgccg
     1861 ccaatttgct ggatgaggcg aacgatttct tcgccttctg ccattcgccg tgaagcaatc
     1921 gccagtttgg caccggcttc acaaaatacc tcggccatgg cgcgtccgat tccagttgcc
     1981 ccacctgtga tgagtactat ctgtccatcg agtcgaaaca taagcagaat gaagaatgaa
     2041 gaatgaagaa tgaagaatgt ggtgaatgct tggtgactgg tgcttggtgc ttggtgcttg
     2101 gaacttggtg cttagtactt gcgaaccaat aatgccagtt aagggttgaa aaaattgttc
     2161 attgaaaaca aagggcttat caaaatctca aatttcagat gttcaatctg ctgtctgtaa
     2221 gttgttgatt ttgaagtctc aatttcaact cttaattcgc atcaatacct tccaagaacc
     2281 aagaaccaag aaccaagaac caagaac
//
LOCUS       DAOQDO010000970         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42729_length_2304_cov_2.859048, whole genome shotgun sequence.
ACCESSION   DAOQDO010000970 DAOQDO010000000
VERSION     DAOQDO010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42729_length_2304_cov_2.859048"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PKZ53_14870"
     CDS             complement(<1..1083)
                     /locus_tag="PKZ53_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41774.1"
                     /translation="MILRSISLLILILLVMAAAGCQQETPLHEAETRPIPAHAALFPE
                     NNQLIEGTTQIAEYRVVRLKCWYAQDDVLAPLIAIRSFHCGRQEYLLTVDFNTLHTQI
                     IKAEYLRTEPCAFGDFAKTSFGQLLSTATTPPFPLQNDGLQALSKVSRYPVLTVDLCP
                     TPKSFERELFEKLIACPLPHPIPVTVCIAGLWIKHHPAELQWLKQMVYERKLDITWAN
                     HSFHHHVQEHAPLQSNFLLMPHTDLNEEILELEKSLIEHGITPSVFFRFPGLVSDQHL
                     VEMLCEYSLVPIGSNAWLAKGQSAQPGSLLLVHGNGNEPLGVRKFLALLNSSSLSGLE
                     SLPEAASQEFKTDGLAQNYSGESTPST"
     gene            1310..2263
                     /locus_tag="PKZ53_14875"
     CDS             1310..2263
                     /locus_tag="PKZ53_14875"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HNJ41775.1"
                     /translation="MKVFIVHAHHEPQSFNGALTRLAQQVLTEAGHEVQVSDLYAMKF
                     DPVSDRRNFTTVKDASFLNQQAEEQFATDNHSFAPEIEAELQKLEWCDVLIFQFPLWW
                     FGMPGVLKGWIDKVLAFGRVYGGGKWYENGIGKGKRALVSMTTGGPEAMYSGFGLNPK
                     MTDLLIPIHHGVFWFNGFQPLPPFIAWSARRVSQEQRTAYLEQYRNRLLTLDTVEPIY
                     HFPTTEFDNSFMDPHQRFMVSWTRTTEPDDQFYTLVAAEREALNHLKNTGKYLSGWIA
                     EDRSRGWLMMRETSEAALREHLERLPLAPYLHFEITVTAKE"
BASE COUNT          599 a          560 c          601 g          544 t
ORIGIN      
        1 ggttgagggt gtactttctc cggaataatt ctgagcaagg ccgtccgtct taaactcctg
       61 actggcagct tccggcagtg attccaaccc cgaaagtgac gatgaattca gaagcgccag
      121 gaacttgcga accccaagcg gctcatttcc attgccatgc accagcaaaa gactgccagg
      181 ctgggccgat tggcctttgg ccagccaggc attgcttcca attggtacca gcgagtattc
      241 gcaaagcatt tcaaccagat gttggtctga gaccagccca gggaatctaa aaaaaaccga
      301 aggtgtgatc ccgtgctcaa tgagggactt ttccaactca agaatttcct catttaagtc
      361 agtatgcggc atgagcaaaa agttactctg caacggggca tgttcctgga cgtgatgatg
      421 gaaggaatga ttggcccagg taatatcgag tttccgttca taaaccattt gcttgagcca
      481 ttgcagttcg gcggggtgat gcttgatcca caatccagca atacagacgg tcacggggat
      541 tgggtgtggc aatggacagg caatcaattt ctcgaacagc tcgcgttcaa aagactttgg
      601 cgtaggacat aagtcaacgg tgagcacggg ataccggctg actttcgaca gagcctgcaa
      661 cccatcattt tggagcggaa acggaggtgt cgtcgccgtt gaaagaagct gaccaaatga
      721 cgttttggcg aaatctccaa aggcacaggg ttcggtccgc agatactcag ctttgataat
      781 ctgggtgtga agggtattga aatcaaccgt gagcagatac tcctggcggc cacagtgaaa
      841 gctccgaatg gcaatcaacg gcgccaggac atcatcttgt gcataccagc atttgagccg
      901 aaccacccga tactcagcaa tttgggttgt cccctcaatc aactgattgt tttcaggaaa
      961 taacgctgcg tgggctggaa tcgggcgggt ttctgcttca tgcaatggcg tttcctgctg
     1021 gcagcctgct gccgccatca ccagcagaat caggatcaat aaggaaatcg agcgcaatat
     1081 catcaggttc tctctgatta taaagagtga attcgaaaca atatcaaaaa ggagagcaat
     1141 ttgtattccg aaccgttcac aagatcccta tcggctcaga gagctggaat tttggatttt
     1201 cagctcccac gtgatggggt aagttggcgc gtgggggaac aaaaatgcct cacacactga
     1261 tgaattcatt ctggttttct caaacaaagg acaaaaagaa aggtacccga tgaaagtttt
     1321 tattgttcac gcccatcacg agccacagag ttttaatggt gcgctcacgc gactcgccca
     1381 gcaggttctg actgaagctg gtcacgaagt ccaggtttct gacttatatg ccatgaagtt
     1441 tgacccggtg tctgaccggc ggaattttac caccgttaaa gatgccagct ttctcaacca
     1501 gcaggctgaa gaacagtttg ccactgacaa ccatagtttt gcccctgaga tcgaagctga
     1561 actccaaaag ctcgaatggt gcgacgtgct gatctttcaa tttccactct ggtggtttgg
     1621 gatgccgggc gttctcaaag gctggattga caaggtcctt gcctttggcc gggtgtatgg
     1681 tggcggaaaa tggtacgaaa acggaattgg caaggggaaa cgcgccctgg tttcaatgac
     1741 cactggcggg ccggaggcaa tgtacagcgg atttgggttg aatccgaaaa tgacggattt
     1801 gttgatcccg attcaccatg gcgtgttctg gtttaacggg tttcaaccac tcccgccatt
     1861 tattgcctgg agtgcccggc gcgtgagcca ggaacagcga acagcgtatc tggagcaata
     1921 tcgcaaccgg ctcctcacgc tcgataccgt cgaacccatc tatcactttc caaccactga
     1981 atttgataat tcgttcatgg atccacatca gcgctttatg gtgagctgga cgcgcaccac
     2041 tgaacccgac gaccagtttt acacactggt ggctgccgag cgcgaagccc tgaaccacct
     2101 caaaaatacc ggaaaatatc tctcaggatg gattgcggaa gaccggtcac gcggatggtt
     2161 gatgatgcgt gaaacctcag aagcggctct gcgcgagcat ttggaacgct tgccactggc
     2221 gccatattta cattttgaga ttacggtgac ggcaaaagaa taatggcagt cagtagtcca
     2281 tcagtcagta gtcagtagtc agta
//
LOCUS       DAOQDO010000971         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42770_length_2303_cov_2.616548, whole genome shotgun sequence.
ACCESSION   DAOQDO010000971 DAOQDO010000000
VERSION     DAOQDO010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42770_length_2303_cov_2.616548"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKZ53_14880"
     CDS             complement(<1..692)
                     /locus_tag="PKZ53_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ41776.1"
                     /translation="MVVIGLVFGLVFLPGLHQGREWFGTSRANSQSPQFSPVQPVSIL
                     ATGRGNPVVQVNDGQPAYHLTKSGVADDGSANLIPLALVAGDFNQDGVPDVVSTFTDG
                     FQGMVNVHLGNLDGLYANSEEVQARVAAGQGSTDSFFPPLPTVTVPEPAQFVATGDFN
                     FDGKMDLVTAANGGTALYWLAGNGQGSFTEAERIEVDGLITAFACGEFNQADGAVDLV
                     VGVNRQEGAAVLV"
     gene            complement(1279..>2303)
                     /locus_tag="PKZ53_14885"
     CDS             complement(1279..>2303)
                     /locus_tag="PKZ53_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ41777.1"
                     /translation="PNATYTPSAGEIAAGTVTLTCTTDDPPGPCLAASDSMTISITSC
                     IGLMVADTTNNRIQGFNGTTWTVVGTGIVGSGDGQFRTPEAVTFGPNRRIYVADTGNN
                     RIQWSTDNGVTWANFATFGTGLNQVKAPQGLELDTDGNLYVSDTGNGRVLRFQGGLPG
                     PGVVIASNGIGGGQVSSPRGLAIDSAFRLFVTDESNSRILRISNANTVTNPATGAILA
                     SQGIGMNQVKNPQGVALDSSGTLYVADTGNSRILRWVNASPANATTMALIGSGLGQVN
                     RPEGVTVVEFTSGQFAGGLVLVVGDTSNNRIQGRFLPTGQWNLVGAPNGIGTGTGQFR
                     APSKVR"
BASE COUNT          536 a          654 c          572 g          541 t
ORIGIN      
        1 accagcaccg ccgcaccttc ctgccgattg acaccgacca caagatccac tgcaccgtcg
       61 gcttgattga attcaccaca ggcaaaggcg gtaatgaggc catcaacctc gattcgctcg
      121 gcttccgtaa agctaccctg tccattgcct gccagccagt acagcgccgt tccaccattt
      181 gccgccgtca ccagatccat cttcccatca aaattgaaat ctccggttgc gacaaactgg
      241 gctggttccg gcacggttac ggttggcaat ggcggaaaga acgaatcagt gcttccctgt
      301 ccagccgcga ctctggcctg aacttcttct gaatttgcat acagtccatc cagatttccc
      361 aggtggacgt tcaccatccc ttgaaacccg tcagtaaagg tactcaccac atccggcacg
      421 ccatcctggt tgaaatcccc agcgaccaat gccagcggga tcaagtttgc agatccatca
      481 tccgccacac ccgattttgt caggtgatat gccggttgac cgtcattcac ctggacaacc
      541 gggtttccac gaccagtcgc caggattgaa accggctgta ccgggctaaa ctgcgggctc
      601 tgggagtttg cccgtgatgt gccaaaccat tcccttccct gatggagtcc gggaagaaag
      661 acaagtccaa acaccagacc aatcacaacc atcacgagcc agattccttg aggtcgagct
      721 ttcaaacctg agcggtataa ccaattccaa gagtgggggg atgacatacg taactatctc
      781 ctttgactga caatgggcag agatcgaaaa tcacttattg atttgggaga tcaagactgg
      841 aggatcacag tgctccaaat ctgattccgt agaatgtaag tgcgcgtttt tcaaggttaa
      901 aaactgaagc aaagaaatgt gaactggagg gaaatttgga ttgcggcatt atgaaattcc
      961 tgaggcccag tcgttaaagt catttttcag gtataagttg cacagaagaa aaagacgcgg
     1021 ggatgcatct tcattatgcc tgtaatattg aagaatggaa caatcttttc gaccagcggc
     1081 ctgttttttc gataaatggc ctgttttttc gataagtagc accttgttag aaaaaaaaag
     1141 aaatgcggca gatctctgcc gcatttggag tgcggcggct taacgccgct tttcttcttc
     1201 tgtttggagt gcggtggctt gacaccgctt ttcttcttct gtttggagtg cggtggcttg
     1261 acaccgcttt tcttcacctc agcggacctt actcggcgcc cggaactgcc ccgtgccggt
     1321 cccaattcca ttcggcgcgc caaccaggtt ccattgtccg gttgggagaa atcggccttg
     1381 aatacggttg tttgaagtat cgcccaccac caggaccagt ccaccagcaa attgaccgct
     1441 ggtaaactca accacggtga caccttccgg gcggttgacc tgacctaacc ccgaaccaat
     1501 cagcgccatc gtcgtcgcat tggctggact ggcattgacc cagcgcagaa tgcgggaatt
     1561 ccccgtgtcc gctacataca gagtccccga tgagtcaagt gccacgccct gtgggttttt
     1621 cacctgattc attccgatgc cctggctggc gagaatggcg ccagtcgccg ggttagttac
     1681 agtgttggca ttgctgatgc gcaggatgcg gctgttactt tcatctgtta caaacaagcg
     1741 gaaggcactg tcaatggcca atccacgcgg actactgacc tgcccgcctc cgatcccatt
     1801 actggcaatc acgacacccg gccctggtag accgccctga aaccgcagta cccgtccgtt
     1861 tccagtatcg gaaacataca ggttgccgtc ggtatcaagt tcgagccctt gtggtgcttt
     1921 cacctggttg agccccgttc caaacgtggc aaaattcgcc caggtcacac cgttgtcggt
     1981 ggaccactga atccggttgt tgcccgtatc agcgacataa attcggcggt ttggaccaaa
     2041 cgtcacggct tcaggggttc ggaattgacc atcacctgaa ccgacgattc cagtccccac
     2101 cacagtccag gttgtgccgt taaagccctg gattcgatta ttggtcgtgt cagccaccat
     2161 caacccaata cacgaagtga ttgaaatggt catggaatct gacgccgcaa ggcacggccc
     2221 tggtggatca tccgtggtgc aggtcaaggt taccgttcca gccgcgattt cacccgccga
     2281 cggcgtgtag gtggcatttg gcg
//
LOCUS       DAOQDO010000972         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42782_length_2302_cov_10.941700, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010000972 DAOQDO010000000
VERSION     DAOQDO010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42782_length_2302_cov_10.941700"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1819)
                     /locus_tag="PKZ53_14890"
     CDS             complement(146..1819)
                     /locus_tag="PKZ53_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41778.1"
                     /translation="MMTIASSRSPFAMNRLSLENTLIDDRFEVLRCIAQGSYAEIFIA
                     TDRLNGATVIIKALNTSLQGSLEPELDRVLVENFETEAQVLDTIRHPNIVRRLGHGMA
                     ADLQGKTFYFLVLEYMAGGNLLDFCRSHGPLSLEETLFYFQQVAEGLEHAHESQIIHR
                     DMKPSNLLLSQDLKIIKIADFGVAKIAHREDEEITRVGTNVYAPPEHHPNFDGQITWG
                     RRLTPAADIYALAKTIYTVLSGKAPQMFNGRPITALPHPLTTLPYSQELLRILRKATA
                     TDSSERYPDVESFWADFARLGEWQEDEATRVQPRSARSAPLEIPEAPPAAHFIPATQI
                     SPAAAEHGETRLLGSVSASESHGPIPKPVSRIVIPIDSPESAQPVSLRKGNAPAEQPR
                     GGKSFIPLSIPEAALPQTTPPESSQPDTVPEIPVPAQPSRWRAIFTPGVIHWLTTLAI
                     AIFFVTSLVIIYRTTKQYVADFQFSRTRGIWSRPTRYAVTTTGVNVRSAPRVENNVVG
                     ELARGVRVEILEEQGRWYRVKPVEWVRRSDTSVQDGWVSSQFFGPTSSE"
BASE COUNT          555 a          548 c          658 g          541 t
ORIGIN      
        1 tgaagaattg agaatgaaga atgaagaatg aagaatgaag aatgaagaat gaagaggagc
       61 cgggttactg gtatcgaaaa cccggaaccc ggaacccgga acccggaacc cggaacccgg
      121 aacccggaac ccggaaccca attcttcatt ccgagctggt cggaccaaaa aactgggagc
      181 tgacccagcc atcctggacg ctggtatctg agcgtctgac ccattccact ggtttcactc
      241 gataccagcg gccttgttct tcgaggattt ccactcgaac gccacgcgcc agctcaccaa
      301 cgacattatt ttcaacccgt ggcgccgaac gcacattgac gccagttgtc gtcaccgcat
      361 agcgagtcgg tctggaccag ataccacggg ttcgtgagaa ctgaaagtcg gcgacatatt
      421 gtttggtggt gcgatagatg atcaccagac tggtgacaaa aaagattgca atcgccaatg
      481 tggtcaacca gtgaataaca cctggcgtaa aaatggcccg ccatcgggaa ggctgagccg
      541 gaactggtat ttctggtacc gtgtctggtt gtgatgactc tggcggagtg gtctgaggga
      601 gggccgcttc aggaattgaa agtggaataa atgatttgcc gccacgaggc tgttcagcgg
      661 gagcattgcc tttgcgaagg gaaacaggtt gcgctgattc aggcgaatca atcgggatga
      721 caatccgact gactggtttc gggatggggc catgtgattc tgaggctgag actgaaccga
      781 gaagccgggt ttcaccgtgc tcagcggcag ccggggaaat ttgggttgct ggaataaaat
      841 gagcggctgg cggagcttcc gggatttcaa gtggggcgct gcgggccgag cgtggttgaa
      901 cgcgggtggc ttcatcctcc tgccactctc cgagacgggc aaagtcggcc caaaacgatt
      961 caacatccgg gtatcgctca ctagaatccg tagccgttgc ttttcgcaga atgcgcaaca
     1021 attcctggct atagggaagc gtggtgagtg ggtgcggcag ggctgtaatt ggccttccat
     1081 taaacatctg gggcgctttt ccactcagaa ctgtgtaaat tgtcttggcc agggcataaa
     1141 tatcggcggc tggcgtcagt cgtcgtcccc aggtaatctg accatcaaaa tttggatggt
     1201 gttcaggcgg ggcatagacg ttggtaccca cacgggtgat ttcttcatct tcacgatggg
     1261 caattttggc cacaccaaag tcagcaattt tgatgatttt caaatcctgc gaaagcagca
     1321 ggttggacgg cttcatgtcc cggtgaatga tctgtgattc gtgggcgtgt tcgagccctt
     1381 cggcaacttg ttgaaagtaa aacagggttt cttcaagtga gagcgggccg tgtgaccggc
     1441 agaaatcaag caggtttcca cctgccatat attcaagtac aagaaaatag aaagttttcc
     1501 cttgcaggtc agccgccatt ccgtggccaa gccgccgcac aatattcgga tgacggatcg
     1561 tatcgagtac ctgcgcttcg gtctcaaaat tctcaactaa aacccgatcc agttctggtt
     1621 cgagcgagcc ttgcaggctg gtattgaggg ctttaatgat caccgttgcc ccattgaggc
     1681 ggtcagtagc aataaaaatc tcggcatagc taccctgggc gatacagcgc agcacttcaa
     1741 aacggtcatc aatgagtgtg ttttcgagag aaagtcggtt catggcaaat ggtgatcgag
     1801 aggatgcaat cgtcatcata agtagcccac gaaaaatgaa aagggaaaaa gtaaaagctg
     1861 caaccagatg tggaaatgtg actggttgcc atacgccact tgcccgtgta tgtttcgggg
     1921 acacaggaca ttgggctgag ggtagagggc taagggctga agacttcggg cggaagaaaa
     1981 cgggcttgag gtggaagact cgcaaactcg gggctgaatt atcgaacttc ttcagccctc
     2041 agccctcagc ccaattgctt cagcccgagg tcttcagccc caagccctga gcccaggtct
     2101 tccacccact gttttcaacc tgctttctct gagttttcag tcatgaactt tgtttttacc
     2161 aatcgatttt tgtggctttt tgccgttggg ctgatttttt tagttgtgag ttgggtggtg
     2221 gtgccgctcc gatggctggt actggtctat gacctcgctc tggtggcagc ggcgatgtgg
     2281 gattatcggc gaactgaatc tc
//
LOCUS       DAOQDO010000973         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42791_length_2302_cov_4.124611, whole genome shotgun sequence.
ACCESSION   DAOQDO010000973 DAOQDO010000000
VERSION     DAOQDO010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42791_length_2302_cov_4.124611"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PKZ53_14895"
     CDS             <1..1155
                     /locus_tag="PKZ53_14895"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNJ41779.1"
                     /translation="VLGAGRQGTASGYDIARFGDAEAVIMADSNGKVAEESAARINRL
                     VGKEIARGVELNAKDPAAVAAYFEPLDSCVSAIPYFLNFEATKAAIAAKCHFCDMGGN
                     TGVVMQQLGLHADATQAGVSVVPDCGVGPGMISNLAVYLAEQFEVAEDILIYDGGLLQ
                     QPRPPFNFALFFNIEGLTNEYWGDALYLVDGKVKPTACFADDEYELIEIPGLGMFEAF
                     VTSGGLSTMVPTYEGKLKTLKNKTLRYPGHVALFKGLRDIGLFNLDPIEVGGVSVVPR
                     QVLHTLLDPKIAPLPEDKDMMVIHIVGRGQKGGAPAEIHLDIVDRLEETTGFYAMERM
                     TGFHAAIVAQMMARGEVAQGAVSLEKAVNATRMVEEMAKRDMNVTLTQVK"
     gene            1448..>2302
                     /locus_tag="PKZ53_14900"
     CDS             1448..>2302
                     /locus_tag="PKZ53_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HNJ41780.1"
                     /translation="MNFLSEFSTGRFERAHQRIASFVRHTPLLPAPLLQENVPDNLIL
                     KLENLQISGSFKIRGVFNTLLQLGEAERERGVITASGGNHGVALAIGAHKLGIRCLVY
                     LPATADNDRVQRVERWGATVIKHGLAWDDANAQALLHAEQDNLTYVHPFDAIQTIEGQ
                     GTLGLEILEDVPDVDCVLVAIGGGGLISGVSTAIKLKKPEVQIIGVEPVGAPSMLESI
                     NNGSVRKLSEVRTVATTLAPRSVSERTLALSQSFVNQIVLVTDDQMVDAMRWLWVECN
                     QLVEPAGAA"
BASE COUNT          588 a          515 c          614 g          585 t
ORIGIN      
        1 gttttgggcg ctggccgtca gggaacagcg tcagggtatg atattgcacg gtttggcgat
       61 gctgaagccg tcattatggc tgattcaaat gggaaagtgg ctgaagaaag cgccgcgcgg
      121 atcaaccgcc tggtgggcaa agaaattgcc cgtggcgtgg aactcaacgc gaaagatcca
      181 gccgccgtgg ctgcttattt tgagccgctg gattcttgcg tcagtgcaat tccgtatttt
      241 ctcaactttg aagccaccaa agctgccatt gcggccaaat gccatttttg cgatatgggt
      301 ggcaacactg gcgtcgtgat gcagcaactt ggactgcacg cagacgccac ccaggctggt
      361 gtttcagtgg ttccagattg cggcgtcggc ccaggcatga tttccaatct cgccgtctat
      421 ctggcggaac agtttgaagt ggccgaagac atccttattt atgacggtgg attgctccag
      481 cagccacggc ctccgtttaa cttcgcgtta ttttttaaca ttgaaggatt gaccaatgaa
      541 tactggggtg atgccctgta tctggttgat ggcaaagtca aaccaacggc ctgttttgct
      601 gacgatgaat atgaactgat tgaaattccg ggcctgggaa tgtttgaagc gtttgtaacc
      661 tcaggcgggc tttcaaccat ggttccaacc tatgaaggaa aactcaaaac gctgaaaaat
      721 aaaaccttac gctatcctgg acacgtggct ttgtttaaag gactgcggga tattgggttg
      781 tttaacctgg atccgattga ggttggcggc gtgtcggttg tgccgcgtca ggtgctccat
      841 acacttctgg atccgaagat tgctccactc cctgaagata aagatatgat ggtcattcat
      901 atcgtgggcc ggggtcaaaa aggcggtgcc ccggctgaaa tccacctgga tattgttgac
      961 cgactggaag aaaccactgg tttttatgcc atggaacgca tgaccggatt ccacgcagcg
     1021 attgtggcgc aaatgatggc gcgcggagaa gtcgcccagg gcgcggtttc ccttgaaaaa
     1081 gcagtcaacg ccacccggat ggtcgaagaa atggccaaac gtgacatgaa cgtcacgctc
     1141 acacaggtaa aataaggcag gaagaattga gaatgaagaa ttgagaatga agaatgaaga
     1201 attgagaatg aagaatgaag aattgagaat gaagaaagtg gttaatactt ggtgacgggt
     1261 acctggttct taggaatcaa tacgttcgaa gaaaaaccaa aaaccaagaa ccaagaacta
     1321 agaaccaaga actaagaacc agtattttct tcattcttca ttcttcattc ttcattcttc
     1381 attctccatt cttcattctt cattcttcat tcttcattct gtttatcccg tttgtttcca
     1441 aaattttatg aattttttaa gtgaattttc gaccgggcgt tttgagcggg cacatcaacg
     1501 gattgcatct tttgtcaggc ataccccact cttacctgcg ccgttactgc aggaaaatgt
     1561 cccagacaat ctgattttaa aacttgagaa cctgcaaatc agtgggtcat tcaaaattcg
     1621 gggtgtcttc aatacactgc ttcaattggg tgaagccgaa cgcgaacgag gggtcatcac
     1681 ggcttcgggc ggaaaccatg gtgtggcact ggccattggg gctcacaaac ttgggattcg
     1741 ctgcctggtg tacctcccag ccaccgccga caatgaccgg gttcagcggg ttgaacgatg
     1801 gggtgctacg gtcatcaaac atggccttgc ctgggatgac gccaatgccc aggccctgct
     1861 tcacgctgag caggacaacc tgacctatgt tcacccattt gacgcaattc agacgattga
     1921 aggtcagggc acgcttgggc ttgaaatcct cgaagatgtt ccggatgttg attgtgtgct
     1981 ggtggcaatt ggtggaggcg ggttgatttc aggtgtttca acagcgatca aattgaaaaa
     2041 acctgaggtt cagatcattg gtgtggaacc ggtcggagcg ccttcgatgt tggaaagcat
     2101 aaacaatgga tcggtgagga aattatcgga agtacgtacc gtggcaacca ctcttgctcc
     2161 aagaagtgtg agtgaacgaa ctctagcact ctctcaaagc tttgtaaatc aaatagttct
     2221 ggttaccgat gaccagatgg tagatgcaat gcgctggctt tgggttgaat gcaaccagct
     2281 tgtggaaccg gctggggcgg ct
//
LOCUS       DAOQDO010000974         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42794_length_2302_cov_3.508678, whole genome shotgun sequence.
ACCESSION   DAOQDO010000974 DAOQDO010000000
VERSION     DAOQDO010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42794_length_2302_cov_3.508678"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..552)
                     /gene="rnpB"
                     /locus_tag="PKZ53_14905"
     ncRNA           complement(116..552)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKZ53_14905"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(597..683)
                     /locus_tag="PKZ53_14910"
     tRNA            complement(597..683)
                     /locus_tag="PKZ53_14910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Ser,seq:gga)
     gene            complement(769..858)
                     /locus_tag="PKZ53_14915"
     tRNA            complement(769..858)
                     /locus_tag="PKZ53_14915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(822..824),aa:Ser,seq:cga)
     gene            complement(837..1340)
                     /gene="tadA"
                     /locus_tag="PKZ53_14920"
     CDS             complement(837..1340)
                     /gene="tadA"
                     /locus_tag="PKZ53_14920"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNJ41781.1"
                     /translation="MTETPSDVFWMEIALNEAQASARQDEVPVGAVLVFDNQLIARSG
                     NRTLRDQDPTAHAEIVTLRTAAQVLGNHRLLDTTLYVTLEPCAMCAGALIQARVKRLV
                     YGASDLKAGAVDSHFGICSAAFLNHRLEITSGLLGNECSQVLQEFFRAKRQAKQAERT
                     LVRCESG"
     gene            1485..>2302
                     /locus_tag="PKZ53_14925"
     CDS             1485..>2302
                     /locus_tag="PKZ53_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41782.1"
                     /translation="MKTNMIHSWVLDFCRTIQQHNGRAFLVGGYVRDQLFERQSKDYD
                     LEVYGIPGDSLRALLKEMGRVNTVGEQFTVYKFRPLVDPETEIDVSLPRRESKTGVGH
                     RGFTIEGDPFMSFHEATRRRDFTINALMLDPLTGEIIDPQQGQQDIKRRYLRVVDPET
                     FVEDSLRVLRGAQFAARFELAIDPDTVALCRSISLTDLPHERIWGEFEKLLLKSRRPS
                     IGMAALNDLGVVDQLFPELAALRGCQQEPEFHPEGDVWIHTLMCLDEAAHLIDDL"
BASE COUNT          531 a          620 c          587 g          564 t
ORIGIN      
        1 acttcttcag cccgatgtct tcagcccgac ttcttcagcc cgatgtcttc agcccaatgt
       61 tcttcaaccc caaaagaaaa tcccctgccg gttttgcaac caggcagggg attttgattg
      121 gcacaggtct ataagccgaa ttctgtggtc accaggaaag gtgactggtg atcattcatc
      181 taggccggca attgctcgac ggctcaagcg acttacccgg aagctgcgtc cacacagtgt
      241 tccaaagaac actggtgaaa ctcggacggg caaccctcaa acgcttccct atttagtctt
      301 gcactgcgag gagtttacct agccagatct gttaccagac ctgctggtgg gcctttaccc
      361 caccgtttca ccatcgccca ccatgcctcc cgagggaggg gtaggtttcg ggctgtctgt
      421 tttctgttgc acttgtcgtc accggctgac tgacgccaac cagtgcctgg ccgttagcca
      481 gctcgctgcc ctacagtgtt cggactttcc tccctccatc ttgcgacgga cggcgatcac
      541 ccaacctgta ccaaaagtgt gagagccagg aaagctcact ggcttcctgg ctcagacgga
      601 ggaggtggga ttcgaaccca cggtaccgtt tccagtacac agcatttcca atgctgcacc
      661 ttcagccact cggtcacccc tccaggggaa tgaagaatta agaattgaga aatgttcggc
      721 ttaattcttc attcttcatt cttcattctt cattcttcat tcaaaacgtg gcggtgaggg
      781 tgggattcga acccacggta cggtttcccg tacttcggtt ttcgagaccg accgattcaa
      841 ccactctcgc acctcaccaa agttcgttca gcttgtttgg cctgccgttt ggcccggaaa
      901 aattcctgca agacctgact gcattcattt cccaaaagtc cgctggtaat ttccagccga
      961 tggttgagaa acgccgccga gcaaattcca aaatgcgaat ccaccgcccc ggctttcagg
     1021 tcactggcgc cataaaccag tcgtttgact cgtgcctgaa tgagcgctcc ggcgcacatc
     1081 gcacagggtt caagcgtgac atagagcgtg gtgtcgagca accgatggtt gccaagcacc
     1141 tgtgccgccg ttcggagcgt gacgatttcg gcatgcgccg tgggatcttg atcacgcaac
     1201 gtacggttgc cgctccgggc aataagctga ttgtcaaaca caaggaccgc accaaccggg
     1261 acttcgtctt gtctggcact tgcctgggct tcgtttaaag caatttccat ccagaacaca
     1321 tcagatgggg tttcagtcat aatgaaatga attgtgaccg tgattgcttg tgggaaaaaa
     1381 tcgtgtgaaa tgtgtatttg gttcccaaag ccttgaaaaa aagacgaacg gctatggtac
     1441 aagtcaaccg tgaaaactgt caagatagca attttgagga aggcttgaag acgaacatga
     1501 tccattcctg ggttttggat ttttgccgca cgattcaaca acacaatggt cgggcgtttc
     1561 tggttggcgg ttatgtgcgc gatcaactgt ttgagcgcca atcaaaagac tatgacctgg
     1621 aagtatatgg cattcctgga gatagcctgc gggcattgct caaagaaatg ggccgggtca
     1681 acaccgtcgg cgagcagttc acggtctata aatttcgtcc gctggttgat cccgaaactg
     1741 aaattgatgt gagtttgccg cgacgggaat caaaaaccgg tgtcggccat cgggggttta
     1801 ccatcgaagg cgacccgttt atgagttttc acgaagcgac ccgccgccgt gatttcacca
     1861 tcaacgccct gatgctcgac ccgttaacag gtgaaatcat tgatccacag cagggacaac
     1921 aggatatcaa acgacgatat ttgcgagtgg ttgatccgga gacgtttgtt gaagactcgc
     1981 tccgggtatt gcgtggcgca caatttgccg cccggtttga actggcaatt gaccctgaca
     2041 ccgttgcttt gtgtcgctcg atttcactca ccgatctgcc gcacgagcga atttgggggg
     2101 aatttgaaaa actcttgctg aaatcacggc ggccctcgat tggcatggcg gccctgaatg
     2161 atctgggggt cgttgatcaa ctctttcctg aactggccgc cttacgcgga tgtcagcaag
     2221 aaccagaatt tcaccccgaa ggtgatgtct ggatccacac gctgatgtgt cttgacgagg
     2281 cagctcactt gattgacgac ct
//
LOCUS       DAOQDO010000975         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42800_length_2302_cov_3.242546, whole genome shotgun sequence.
ACCESSION   DAOQDO010000975 DAOQDO010000000
VERSION     DAOQDO010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42800_length_2302_cov_3.242546"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="PKZ53_14930"
     CDS             complement(<1..1943)
                     /locus_tag="PKZ53_14930"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41783.1"
                     /translation="MMKKIRAISVLSFTSCLLIQTVFWPSETGATFTAMATHGIQARN
                     PEPGTQNPSSHSLNPGEKHTYPLKLKPRSFLKVTLEQKGIDVVLRLVGPDGKKLCEEN
                     ESTGRYDTEKFSRIFDQAGRYILEVESLNKTDLPGRYEVKIEGMRVATDQDRAQVEIE
                     QLIADAGKLRRGGKYTEAVPLIEKALSQSEKIFGPEHPLVGESLIERGVLHYEQGTYA
                     EAKGCYDRARAIKEKEFGKEHRSIASLLNNLASTALMSGQYEVAESLFVQCRTMQEKT
                     LGPECPEVASTLNNLAALYWRKGNFAKAESLLQQALSLKEKLYGPDHEETGFSLNSLA
                     VMYYIRGNVGKAEPLFVRALAIQEKTLGPDHPSLATTLNNLGTLYQRKKEYETAEAYL
                     LRALDIRRKTLGVNHTEVGISLNNLAVLYRETTNYTQAMNYYLEAIRVQEKALGATHP
                     EVAKPLAGLAVMYQQRGEYAKALPLLERSLAIIEQKFGPDHPDVTTGLNNLAVYYQDV
                     GDFVRAETLYRRSLAIREKALGLNHSNTALSLTNLAVLLHARGNFKEAETVHRQALII
                     REKIFGSDHPEVASSLGNLAALLKDTGDYAASLLLQERALAIWEKTMGPDHPNVGLCL
                     HNLGGLYYELGDRKKAVDFHLRAL"
BASE COUNT          512 a          617 c          534 g          639 t
ORIGIN      
        1 agcgcccgca ggtggaagtc taccgctttt ttccgatctc ccaattcata atagagtcct
       61 cccagattat gcaggcataa ccccacgttt gggtgatctg gccccatcgt tttttcccat
      121 atcgccagcg cccgctcctg aagcaacagt gatgccgcat agtcgccggt atctttcaaa
      181 agtgccgcca gattgccaag gctactggcg acctcggggt gatctgatcc aaatattttt
      241 tcacgaatga tcagtgcctg acggtgtact gtttcggctt ctttgaaatt gcctctggca
      301 tggagcaaaa ctgccagatt ggtcaggctc aaggcggtat ttgaatgatt cagacccagt
      361 gctttttcac ggatcgcaag ggagcggcga tagagcgttt ctgcccggac aaaatcgcct
      421 acgtcctggt agtacacagc cagattgttc agaccagtgg tcacatctgg atgatcaggt
      481 ccaaattttt gttcgatgat cgccagtgac ctttccagga gcggcaaggc tttggcgtat
      541 tcgccccgtt gctgatacat cacagctaaa ccggccaggg gtttggcaac ttcaggatga
      601 gtggccccaa gtgctttttc ctgtacccta atggcctcaa gataatagtt catggcctga
      661 gtatagttcg ttgtctctcg atacagaacc gccagattgt tgagtgatat tccaacctcg
      721 gtgtgattga ctcctaatgt tttccgccga atgtccagcg cgcggagcaa ataggcttca
      781 gccgtctcat actctttttt tcgctgatac agagtcccca gattgttgag tgttgtcgca
      841 agtgacggat gatctggccc aagtgttttc tcctgaattg ccagagcgcg gacaaacaaa
      901 ggttcagctt tcccaacgtt accccgaatg taatacataa ccgccaggct gtttaaacta
      961 aaacccgttt cctcgtggtc ggggccatac aatttttcct tgagcgacag ggcttgctgt
     1021 aagagagact ctgctttggc aaaattccct tttctccagt agagcgccgc cagattattc
     1081 aaagtgctgg caacctcggg acattcgggg cccagggttt tctcctgcat tgtgcgacac
     1141 tgtacaaaca gggattccgc cacttcatac tgaccactca tcagcgcggt actggccaaa
     1201 ttattgagca gactggcaat cgagcggtgt tctttgccaa attctttttc tttgattgcc
     1261 ctggcccggt cataacaacc cttggcctca gcataggttc cctgctcgta atggaggacg
     1321 cctctttcaa tcaacgactc ccccacaagt gggtgctcag gtccaaagat tttttcgctc
     1381 tggctcaatg ctttttcaat cagagggact gcttccgtat actttccacc cctgcggagc
     1441 ttcccggcat cggcgatcag ttgttcaatt tcaacctggg cccgatcctg atctgtggcc
     1501 acccgcatgc cttcaatttt gacttcatag cgaccaggaa gatcggtttt attgagtgat
     1561 tcgacttcga ggatgtaccg tccagcctga tcgaaaatcc gcgaaaattt ttctgtgtca
     1621 tatctgccgg tggattcatt ctcttcacag agcttttttc catctggtcc gaccagccgc
     1681 agcaccacat caattccttt ttgctcaagg gttactttca gaaaacttct gggtttgagc
     1741 ttgagtggat aggtatgctt ttctcccgga ttcagggagt ggctactcgg gttctgggtt
     1801 ccgggttccg ggttccgggc ttgtatcccg tgcgttgcca ttgccgtgaa ggttgccccg
     1861 gtctctgacg gccagaaaac agtctggatg aggagacaac tcgtgaaact caatacactg
     1921 attgccctga tttttttcat cattgccgcc ccctccctgg tgtttacagc cttctcagta
     1981 ccttaccgaa agttaccagg tattttaacc acggaaaaca cggaaaacac ggaaaaaaat
     2041 caagcactta ccaaacccaa tctctcagga aactgatgac agggtactta taccaatttg
     2101 ggctgagggt atcgggctaa gggctgaggg aaatacaatt ctttcaacaa tttagccttc
     2161 tcgtaatacg atggcttcat tccaaagtgg tattactatc gctaccttca ggacgcaacc
     2221 tcttatcctg gcgctcagtc caatttcttc agccccaagc cctcagcccg atgtcttctg
     2281 ccctggtttt ttcagcccca ag
//
LOCUS       DAOQDO010000976         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42845_length_2301_cov_2.768477, whole genome shotgun sequence.
ACCESSION   DAOQDO010000976 DAOQDO010000000
VERSION     DAOQDO010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42845_length_2301_cov_2.768477"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKZ53_14935"
     CDS             <1..587
                     /locus_tag="PKZ53_14935"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ41784.1"
                     /translation="EPLQIRWQSSDAGGALASHAIDLSVDGGTTFGTSVAAGLGATAQ
                     SFVFQIPTTIASATARIRVTARDTAGNNGQDTSDANFTIKSTDTVAPTVTVTAPAAGQ
                     KVKVTGSSSFTVTWTSSDNIGVASHDVLLAKDGTTFQNLATGLSGTTTTLSLTQSLVG
                     PAGKSKAAKLRVVARDAGGNSGTGETGAFKLIVK"
     gene            739..1257
                     /locus_tag="PKZ53_14940"
     CDS             739..1257
                     /locus_tag="PKZ53_14940"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNJ41785.1"
                     /translation="MRLCSNLLICFCGSLLLIAGLGSGPVSAQEKTAPKTTLAELAFI
                     AGTWQGKLGEGTTEEQWNPPSGDSMMGMFRYVQNGKAAFYEFMLIEQTPDGPVLRLRH
                     FNPRLIAWEDKTGTLNFSLVEFRKNYAVFDHDSKERLIFHRNSTTSMTITLEKTEGGQ
                     KTAEDFTYRLKK"
     gene            1751..>2301
                     /locus_tag="PKZ53_14945"
     CDS             1751..>2301
                     /locus_tag="PKZ53_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41786.1"
                     /translation="MNQGKWRATSGLRLITLTCLLALIAASVSIRIGQSLTALADQQP
                     ASAQKNPPTAGESSQPAPKSPIPVGALSRTPLRPGEMMRRQDGAKFIEIRQEEFPSTG
                     QQIKGEPGTATRKAEIDFSEIAREEAAGLIQKVSKPKKSTVRIFPVTDPHPDNQIIVS
                     PPATIIAPQPEADRKVNVQPRVPS"
BASE COUNT          586 a          611 c          580 g          524 t
ORIGIN      
        1 gcgaaccact ccagattcgc tggcaatcat ccgatgccgg tggagcgctc gcttcacacg
       61 cgattgatct ttcagttgat ggtggaacga cctttggcac gtcagtggct gctggactag
      121 gcgccacggc ccagtcattt gtcttccaga ttccaaccac gattgcctca gccacggccc
      181 ggattcgggt gacggcccgc gatacggctg gaaacaatgg tcaagatacc tcagacgcta
      241 acttcacgat caaatcaact gacaccgttg ccccaacggt gaccgtaacg gcacctgccg
      301 ctggtcaaaa ggtcaaggtg acaggttcca gctcgtttac cgttacctgg acgagcagtg
      361 acaacattgg ggttgccagc catgatgtat tgctggccaa agatggaacg acgttccaga
      421 atctggccac aggactctca ggcacgacca caacgttgag tttgacccag tcgctggttg
      481 ggccggctgg aaagagcaaa gcggcaaaac ttcgggtggt ggccagggac gctggtggca
      541 atagcggcac cggtgaaact ggcgccttta aactcattgt gaaatagaca ccaggcttga
      601 gaacttttgg ttactcttta aggaggacac tctcggaaac acaggggaaa atcaagattc
      661 ctcagtattc cagatgtgtc cttcttctgt atttccagac cctgacagtc tggttctttt
      721 gcttgatgag gggggaatat gcgtctgtgt tcaaacctgc tgatttgttt ttgtggctcc
      781 ctgttgttga ttgctggact gggctcaggt ccggtcagtg cccaggagaa aaccgcccca
      841 aaaacaacac tggctgaact ggccttcatc gccggcacct ggcaagggaa gctgggtgag
      901 ggaacaactg aggaacagtg gaacccgccc tctggcgata gcatgatggg gatgtttcgc
      961 tacgtccaaa acggtaaagc tgcgttctac gagtttatgc tgattgagca aacccccgat
     1021 ggtccggtgt tacggctccg gcatttcaac ccccgactga ttgcctggga ggacaaaacc
     1081 ggcacgttga atttttcgct ggtcgaattc cgaaaaaatt atgctgtgtt tgatcatgac
     1141 agcaaagagc gtctgatttt tcaccgcaac tccacaacct caatgaccat cacccttgaa
     1201 aaaacagagg gcgggcaaaa aacagccgaa gattttacct accgactcaa aaaatagctg
     1261 atttggcggg cgccgaggtg ctggcgcttt caactgtccc acctcacatt ttctgaaaat
     1321 ctgcacaata tcggctgtcc tgattcgtca cacctccgga gaatgtgtac aatacaggtg
     1381 aaccgttcct ttcccaccgc gcacagcgat tttcttgaaa caacaataaa gagttttcaa
     1441 tccttgctct tgcagcaggc ggtcaaacaa cccaactcat ttttctgtcg tgtctcaact
     1501 ggcccaggag aagtatgggc cagcagaatc ttaccgatga ggaaacccag gccctcttca
     1561 accggtgtgc gaccattcaa gtacaaacat aatcttcaac gtcatttcgt gacgagcagc
     1621 aggtgcaaaa accacaacaa gaatcttctt gatctgagct agaagagccg ttctctggct
     1681 ttggtctgga tttggtgcat aagaaaagtg gtagcccggc ttgtctcatt ttctaagaaa
     1741 gaggtgcagt atgaatcaag ggaagtggag ggcaaccagc ggtcttcggt taattaccct
     1801 cacgtgttta ctggcactta ttgccgcaag tgtctcaatc cggattggtc aatcgttgac
     1861 cgcactcgcc gatcaacagc cagccagtgc ccagaaaaat ccgccaaccg ccggtgaatc
     1921 aagtcagccg gctccaaagt cacccatacc ggttggggct ttgtcgcgta ccccgttgcg
     1981 tcctggagaa atgatgcgcc gtcaggatgg tgccaaattt attgaaattc gacaggaaga
     2041 atttccatca accgggcagc aaatcaaagg tgaacctggg accgccacgc gcaaagctga
     2101 aattgatttt tctgaaatcg cccgtgaaga agccgccggg ttaattcaaa aagtatcgaa
     2161 gccgaaaaag agcaccgtcc gcattttccc ggtcaccgat ccccacccag acaatcaaat
     2221 tattgtcagt ccgccggcta ccatcattgc tccacagcct gaagctgacc gcaaagtgaa
     2281 tgtgcagccg cgagtgccat c
//
LOCUS       DAOQDO010000977         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42907_length_2299_cov_3.757576, whole genome shotgun sequence.
ACCESSION   DAOQDO010000977 DAOQDO010000000
VERSION     DAOQDO010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42907_length_2299_cov_3.757576"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1062
                     /gene="mreC"
                     /locus_tag="PKZ53_14950"
     CDS             202..1062
                     /gene="mreC"
                     /locus_tag="PKZ53_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNJ41787.1"
                     /translation="MKSDSSLRPTILAVILLVSQFLLMTINAQARITKQSLPRTIILT
                     ATYPIQKGLAVVTTSVSHIWKGYISLWRAAEENQHLRQEVSQLKRERTELQETVASHK
                     RLQELLKLKESFQSPTIAANVIGRDGSPWYKQIIIDQGTLAGIQLNQVVITPDGVVGR
                     IVSIGPSSAMVLLITHGDAGVGAMLAESRTNGELRGRNEAFCQLDSISGLTQVRVGEA
                     VLTTGLDGFYPKGLLLGYVAQVQLGSGAGLHQIAVRPAAQLDRLEEVLVLLSRPSDIQ
                     MTESVKKEKN"
     gene            1243..1683
                     /gene="mreD"
                     /locus_tag="PKZ53_14955"
     CDS             1243..1683
                     /gene="mreD"
                     /locus_tag="PKZ53_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNJ41788.1"
                     /translation="MPLILTVHLGLQRNSSQAIWFGAVAGLCQDVALSSRLLGLSGFS
                     KTAIGYGLWFIGNRFRLDGALLRIFILLASSVANTLMFVGLHFFFAAAPEGMNVRGAL
                     KIGGLQLVSNLVVSLFLFPIFDKVFHEDPYDPTRAPELGRRKLN"
     gene            1886..>2299
                     /locus_tag="PKZ53_14960"
     CDS             1886..>2299
                     /locus_tag="PKZ53_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41789.1"
                     /translation="MPLKSLDSDGLHPYNLHTRFLILQYCIIIAFSVLALRFWNMQVV
                     QHDNYVRQAENNRLRDVPIPAPRGNILDRNGRILVDSRPTFNLMINREDITEQDATIR
                     MLVEEFGVDREYVIQQLHSQAAKSRPIEVKIDITDA"
BASE COUNT          530 a          570 c          597 g          602 t
ORIGIN      
        1 gtgactttga gttgttaaaa cgtgtcagcc tcgaaagtgg gtacggatac aataatcaaa
       61 actaatgcgg gtgatgcccg actgactgcg gtttggaccc cagagacgcc acttcttgac
      121 tggcgtgtcg gggaagccaa aaggcgattt cagccgccgt cagttgcgta ccacgccgct
      181 tccggccagg ggtaactacg ggtgaagtct gattcatcgc tacgtccaac cattcttgcg
      241 gtcattttgc tggtcagtca gtttttattg atgacgatca atgcacaggc ccggatcacc
      301 aagcaatcgc ttccgcgcac gatcattctg acagccacat acccaatcca gaaagggttg
      361 gcggttgtca ctacttccgt gagccatatc tggaaggggt acatcagctt gtggcgagcg
      421 gcggaggaaa accagcacct gcgccaggaa gtttcgcagc tcaaacgcga acgaaccgag
      481 ttacaagaaa ccgttgccag ccataaacgc cttcaggaat tactcaaact caaagaaagc
      541 tttcaaagcc caaccattgc ggccaatgtt attggtcggg atggaagccc ctggtacaag
      601 cagatcatca ttgatcaggg gacgctggcc ggaattcagc tcaaccaggt tgtgattacc
      661 cctgatggcg tggttgggcg cattgtcagc attggtcctt cttcagccat ggttctcctg
      721 attacgcatg gcgacgccgg ggtcggcgca atgctggccg aatcgcggac caacggggaa
      781 ttgcgtggtc gaaacgaagc tttctgccag ttggatagta tcagcggact gacccaggtc
      841 cgggtagggg aagccgtttt gacaaccgga cttgacggct tttatccaaa agggttgtta
      901 ttggggtatg ttgcccaggt ccagcttggg tcaggcgccg ggctgcatca gattgctgtt
      961 cgtccagccg cccagcttga tcgattggag gaagtcctgg tgctcttgag ccgaccatca
     1021 gatatccaaa tgaccgaatc agtaaagaaa gaaaaaaatt aaagactggt gctttttttg
     1081 accagcgacc gattttcaac caatcatcaa tctcaatgag cgaaaaccaa gcttaatcct
     1141 tttttatttg tgccaactct tcgaattata tttgtcattt ttatcattac ggctcttcaa
     1201 acggggctcc cgcaatattg gccggggtgc agttatcttg atttgccatt aattttgacg
     1261 gttcatctgg gcttgcagcg aaactcaagc caggctatct ggtttggggc agttgccggt
     1321 ctgtgccagg atgttgcgct ctcctcccgg ttacttgggt tatcagggtt ttcaaaaacg
     1381 gcaattgggt acgggctctg gtttattggc aatcggtttc ggctggatgg ggcgctgctc
     1441 cggattttta tccttctggc ctcttcagtt gccaacacct tgatgtttgt gggcctccac
     1501 tttttctttg cggctgcccc agagggaatg aacgttcgcg gtgccttaaa aatcggaggg
     1561 ctgcaactgg tgagcaatct ggtggtttcc ctgtttcttt tcccaatttt tgacaaagtt
     1621 ttccatgaag acccctatga tccgacccgg gcgcctgagt tggggcggcg caagctcaat
     1681 taacagatta gggccagtgg cggccttgcc agaatctcga ccctcctccg gccaaatgac
     1741 gggtgaggtt tctggcgtat ctaggaaatt ttcctagatt cagattgaaa aaaagagcaa
     1801 actatttatc cagctcgtgg cttctcggat ggttcaatcc cagacataac tccctcaaaa
     1861 atattcccct tcgtgttgga gcgctatgcc attgaaatct ctcgactctg acggtttaca
     1921 cccctataac ctccacaccc gattcttaat tctgcagtac tgtatcatca ttgcttttag
     1981 tgttctggcg ctgcggtttt ggaacatgca agtggttcag catgacaatt atgtccggca
     2041 ggcagagaac aaccggttgc gggatgtccc gattccggcg cctcgcggca atattttgga
     2101 ccgcaatggg cgaattctgg ttgatagtcg tccgacgttt aatttgatga tcaatcgaga
     2161 agatatcacc gagcaagacg ctaccatccg catgctggtc gaagagtttg gagttgaccg
     2221 tgagtatgtc attcagcaac tccactctca ggcagccaag tcgagaccga ttgaggtcaa
     2281 aattgatatt accgatgct
//
LOCUS       DAOQDO010000978         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42982_length_2297_cov_3.855486, whole genome shotgun sequence.
ACCESSION   DAOQDO010000978 DAOQDO010000000
VERSION     DAOQDO010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42982_length_2297_cov_3.855486"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2152)
                     /locus_tag="PKZ53_14965"
     CDS             complement(<1..2152)
                     /locus_tag="PKZ53_14965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ41790.1"
                     /translation="MTNDDQSTRPVPPSASLTADPLVGTVFEGKYQILAKLGEGGMGV
                     VYRAHHVHMDVDVAIKFLHHNLTEDPSILERFKREARAAVRINHPNATTVMDFGVLPD
                     NTAYLVMEYLEGESLRDRIYRHTMTAAEAIEILSETCGAVDAAHKRGVIHRDLKPDNI
                     FLKREADGKEIVKVLDFGIAKLRDKGEASHASLTEAGMLIGTPHYMSPEQCHGLELDP
                     RSDVYSLGVIAYELLAGELPFSAPTSLGIVLKHINEEPRPLFIVNPNIPRELEKVVLR
                     ALSKRPDNRPATAGQFARELEAACAGNDVVATANLEGEGVAFHTSMLSSPALVEEDRA
                     DTTDAPAIQTSLMSSPLGHAETSDAISVWHTQDGATRETMANRAGPVQIKEKSRTAPR
                     EESGTLIASSSSLAKEEAYVPAAAKPQLAEHAGGTLKMGAPNQPGVRVAQAIASPASS
                     SVTSALISSVFSARNVAIGVVAVIALAIGAYVLVPRSGLVNPSGNINSGPPSPPPAPK
                     FVLPENMIEIKGGTFTMGRNPGPGVPLDETPAHQVPVKAFAIGKYEVTCKEYAEFIKE
                     SGYPAPSNWKDNRYAPGEENLPVTNVSWEDATQYCQWLAKKTGLAYRLPTEAEWEYAA
                     RSDDQRIYPWGNTFYKDNANTSELKFERPLEINSPKHADDKSAFGVLGMAGNVSEWTA
                     SDRSLYPSSTAPSFPDPGETRKIARGGNYIGPKEG"
BASE COUNT          524 a          586 c          601 g          586 t
ORIGIN      
        1 ctccttcttt cggtccgatg taattccctc cacgagcaat tttgcgagtt tccccaggat
       61 ctggaaaaga tggggcagta ctactggggt agagagaacg atcagaagct gtccactcag
      121 aaacatttcc agccattccc aaaactccaa aagcactttt atcatcggca tgtttgggtg
      181 agttgatttc aagcggacgt tcaaacttta attcactggt attggcatta tccttataga
      241 atgtatttcc ccagggataa atccgctgat catctgaacg ggctgcatac tcccattcgg
      301 cttcagttgg gagacgatac gcaagccctg tttttttcgc caaccactga caatattgag
      361 tggcatcctc ccaggacaca ttcgtcaccg gtagattttc ctcacctggg gcatagcggt
      421 tgtctttcca gtttgatgga gctggatacc cgctttcttt aataaactca gcatactctt
      481 tgcaggtaac ttcatatttc ccaatcgcaa aagcctttac cggaacctga tgcgccgggg
      541 tttcatccag aggaacgcct ggtccaggat tacgccccat cgtaaatgtc ccgcctttaa
      601 tttcgatcat attctcaggc aacacaaatt tcggtgctgg tggcggtgat ggcggaccag
      661 agttgatatt gccagatggg ttgaccagac cggaacgagg aacaaggaca taggccccaa
      721 tggccagtgc gatgaccgct accaccccaa tggcaacatt gcgagccgaa aatactgatg
      781 agattagggc cgatgttacc gatgagctag ctggagaagc aatggcctgg gctacccgga
      841 ctccgggctg gttgggtgct cccattttta aggtgccacc agcatgctca gccaattgag
      901 gtttggctgc tgctggaaca taggcttctt ctttggccag tgagctggat gaggcaatca
      961 acgtgccgct ttcttcacgt ggcgccgtac gcgatttttc tttgatttgt accggtccgg
     1021 cgcgattggc catggtctcg cgtgtcgccc catcctgggt gtgccagact gaaatggcgt
     1081 ctgacgtttc ggcatgtccg agcggactgc tcatcaggct ggtctgaatg gccggtgcat
     1141 cagtggtgtc agcccgatct tcttcgacca gggccgggct actgagcatt gaagtgtgaa
     1201 aagcgacgcc ttcgccttcc aaatttgcgg tggcaacgac atcgttgccg gcacaggcgg
     1261 cttcgagttc acgtgcaaac tggccagccg tagctggacg gttatcaggt cgttttgaaa
     1321 gcgcacgcag aacaactttt tccaactctc gtgggatatt cggattgacg ataaagagtg
     1381 ggcggggctc ttcattgatg tgtttaagca caatcccaag cgaagtggga gcactaaacg
     1441 gcagttcccc ggcgagtagc tcatacgcaa tgacacccag actatagaca tccgagcgtg
     1501 gatccagttc aagaccgtgg cattgctcgg gtgacatata atgtggcgtc ccaatcagca
     1561 ttcccgcttc agtcaaactg gcatgcgagg cttcaccttt atcgcggagc ttggcaattc
     1621 caaaatcgag gactttgacg atttcttttc catccgcttc gcgcttgaga aaaatattgt
     1681 caggtttgag gtcgcggtga atcaccccac gtttatgagc tgcatcaacc gccccacacg
     1741 tttccgatag aatttcaatt gcttcagccg cggtcatggt gtgacggtag attcgatctc
     1801 gcagggattc cccttcgaga tattccatga ccagataggc ggtgttatct ggcaacacgc
     1861 caaaatccat gaccgtggtg gcattgggat gattgatgcg aacggcggct ctggcttcgc
     1921 gtttgaatcg ctcaagaatt gatggatctt cggtcaggtt gtgatgcagg aatttaatcg
     1981 caacgtccac atccatatgg acatgatggg cgcgatagac aacccccatc cccccttcac
     2041 caagcttagc cagtatctga tacttccctt caaacacggt tccaacaagc ggatcagcgg
     2101 taagtgatgc agatgggggc acaggtcgag tggattgatc atcgtttgtc atggaagtca
     2161 ggccttcagt gatgattggg atttggggta gtggaatcgc caattatccg ctgaaacgct
     2221 ggttgctgtc aaagcctgct ccaatcgccc ggtgtctcac tcagagtttg aattgttcgg
     2281 caggatggat acccgtc
//
LOCUS       DAOQDO010000979         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_42986_length_2297_cov_3.384924, whole genome shotgun sequence.
ACCESSION   DAOQDO010000979 DAOQDO010000000
VERSION     DAOQDO010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42986_length_2297_cov_3.384924"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKZ53_14970"
     CDS             complement(<1..520)
                     /locus_tag="PKZ53_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ41791.1"
                     /translation="MFNQLIHLAVAPKKKRLGEREKSIFSTAVFFLAFALCLFPSVAW
                     GQAATGQLRGTVTDPSGAILAGATVTVTNQDTGVSRDVTSNESGDYLVTLLPPGTYRI
                     EVKIEGFKQFTLDNVPVRITETTVANAKLEVGAGTSEIITVTSETPLVRTDSATGGRV
                     IEERSLRQLPLPT"
     gene            complement(894..2018)
                     /locus_tag="PKZ53_14975"
     CDS             complement(894..2018)
                     /locus_tag="PKZ53_14975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNJ41792.1"
                     /translation="MNVPLLDLKAQFATLRSELLPAIETVFENQAFILGREVELLEKE
                     IAEYCGVPHAIACASGSDALLLALMALDIKPGDEVITTPYTFFATAGSISRLGAIPVF
                     VDIEPHTFNLDPTQLEQTITPRTRAIIPVHLFGQCADMDAINQIACAHDLPVIEDAAQ
                     AIGAEWKGKRAGSMGLIGCFSFYPTKNLGAAGDAGCLTTLDDGLAARLRALRVHGETT
                     KYYHRYVGFNSRLDALQAVVLRIKLRHLDAWSTARAQNARHYAELIAAHPGLAGKIDL
                     PTILPDVRHIFNQFVIRATSRDALRNFLKEQGIGTEVYYPLSLHQQECFADLGYRRGD
                     FPESERAALETLALPIYPELTAEMRHYVVENLANFYAEMH"
BASE COUNT          604 a          585 c          573 g          535 t
ORIGIN      
        1 gggttggcag cggtaattga cgcaggctgc gttcttcaat cacccgaccg ccggtggctg
       61 aatcggttcg gaccaatggt gtttcactgg tgacggtaat aatttccgac gttccggcac
      121 cgacttccag tttggcattc gccaccgtgg tttcggtaat ccggaccggg acattgtcaa
      181 gtgtaaactg tttgaaacct tcgatcttga cctcaatacg gtacgttcct ggcgggagaa
      241 gggtgaccag atagtcaccg gattcattgg aggtcacatc acgactgacc cctgtatcct
      301 ggttggtgac ggtcaccgta gcccccgcaa gtatcgcccc ggatggatcc gtgaccgtac
      361 cacgaagctg cccggttgcg gcctggcccc aagcaacgga tgggaaaaga cacagagcga
      421 atgccaagaa aaacacggca gttgaaaaga ttgacttttc gcgttcgccc agccttttct
      481 tcttcggagc tacagccaga tgtatgagtt ggttgaacat gtagcaaact ctccttttat
      541 tttctggact cttgcaaacg atgatggttg gaatgcccga tgatggcatc tccgttcaaa
      601 gtgaatgaaa gctgggggaa atgaccaaaa ggccgcacct tccccttagt tcctaagcac
      661 actgaaggcg aagatatcca agtgtgtggg caaactcact gcacaaaagc tggctacggc
      721 tggattatgg tttaggattt gacgagtcag gggaagattt acacaaggtt tagttcttgt
      781 ctagcctttt tggacattag gggggacttc ctgcaactcc ttgacgccgt tgcacaaaaa
      841 tccaaatttc tataggtgaa atccaattct tcagatcttt gagaatctga atttcagtgc
      901 atttctgcgt agaaatttgc cagattttca accacatagt gacgcatttc agccgtgagc
      961 tcaggataaa ttggaagtgc cagtgtttcg agtgcagcgc gctcagattc aggaaaatca
     1021 ccccggcggt atcccaaatc agcaaaacat tcctgttggt ggagtgaaag cggatagtag
     1081 acttcagttc cgataccttg ctccttgaga aaattccgca gggcatctcg gctggtagct
     1141 cgaatcacaa attgattaaa aatatgtcgc acatcaggca aaatcgtcgg tagatcaatc
     1201 ttgccagcca gtcctggatg tgccgcgatg agttcggcat agtggcgcgc attctgagcc
     1261 cgagccgtgc tccacgcatc taaatgacgc aatttaatcc ggagcaccac ggcctggagc
     1321 gcatccagac gactattgaa tccaacatag cgatgatagt acttggtggt ttcaccatga
     1381 acccggagcg cccgcaaccg cgccgccagc ccatcatcga gcgtcgtgag acatccggca
     1441 tcgcccgcag cccccaggtt tttggtcggg taaaaactaa aacacccaat cagccccatt
     1501 gaaccagccc gcttcccttt ccactcagcc ccaatggcct gggcggcatc ttcaatcacc
     1561 ggcaggtcat gggcacaggc aatctgattg atggcgtcca tatctgcaca ctgaccaaac
     1621 agatgaactg gaatgatggc ccgggtccga ggggtaatgg tttgttcaag ctgggtggga
     1681 tcaagattga aggtgtgggg ttcgatatca acaaaaaccg ggatggcgcc aagtcgggaa
     1741 atcgagccag cggtggcaaa aaacgtgtag ggtgtggtga tgacttcatc gccgggtttg
     1801 atatcgagtg ccatcaatgc cagcaacagc gcatccgaac cggaagcaca ggcaatagcg
     1861 tgcggaacac cacaatattc ggcaatttct ttttccagga gttcgacttc acgccccagg
     1921 ataaaagcct ggttttcaaa aacggtttca atcgcaggca gaagttcaga acgaagcgtg
     1981 gcaaactgcg ctttcaaatc aagcaagggg acattcatgg ggattcactc tgggaaacag
     2041 gctggggaac aaccatcaca gggttcattc aaatggcccc aaaaagattg tcttgccccc
     2101 aaaccaaatt caaaaagggg ctaccctagc ctattttccc agcaattgtc taccctttcc
     2161 acacgatcag tggtcaatca gccacatctg gagaaaagca ctctcagaaa attggtcaat
     2221 cattcaataa ctacttgaaa tcaactcaac cttttctgat tcaggtagtt acccaaacac
     2281 taaccggatt actttgc
//
LOCUS       DAOQDO010000980         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43027_length_2296_cov_3.544400, whole genome shotgun sequence.
ACCESSION   DAOQDO010000980 DAOQDO010000000
VERSION     DAOQDO010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43027_length_2296_cov_3.544400"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PKZ53_14980"
     CDS             complement(<1..1020)
                     /locus_tag="PKZ53_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNJ41793.1"
                     /translation="MAEKSTKRVYLFANGTADGNGQMKDLLGGKGAGLAEMMLAGLPV
                     PPGFTITTHSCNEYYSAGKVFPAGLWDEVLAAMKTVEQTTGKGFGNPENPLLVSVRSG
                     AKFSMPGMMDTVLNLGLNDVTVQGLAKLTSNERFAQDAYRRFIQMFGRIVLGIPGEKF
                     DHIFEHAKHHKGVHLDTDLTADDLKEIVVKFQSVVFKETGQSFPSDPYKQLELSIQAV
                     FSSWFGKRAVDYRRVNKIPDDLGTAVNIVAMVFGNMGQDSGTGVAFTRNPSTGERLLF
                     GEYLTNAQGEDVVAGIRTPAKIAHLQDEMPEVYAEFAKVAETLERHYKDVQDLEFTIE
                     RGKLWM"
     gene            complement(1231..1989)
                     /gene="recO"
                     /locus_tag="PKZ53_14985"
     CDS             complement(1231..1989)
                     /gene="recO"
                     /locus_tag="PKZ53_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNJ41794.1"
                     /translation="MPTIETEAFALRTYPFSEHHKVCVFFTRQVGVVRAVAHGSRRLK
                     SKYGAGLELFSQVHLKFREREGRDLVELADCELVKSHFEAAANPEIAAILSYWSELLT
                     EFLPAHQPNDHIYRLLAALLPCVESELDATDALLRYFETWLLKLSGFYPDLSVCVTCG
                     TNFPSQESLYLARDGTPECASCSERRGLVLSPASRHLIGRILRCPPAEFATVIIEPQT
                     GRVLEELNQHLIRTSLERELKAYTVLKQLREFSL"
     gene            complement(2007..>2296)
                     /locus_tag="PKZ53_14990"
     CDS             complement(2007..>2296)
                     /locus_tag="PKZ53_14990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41795.1"
                     /translation="AVVLSPVIPDAQLLAADIRAQANQMDAALRLYQALIRQYPDFDR
                     GFNNYGVVLLRLGKLTEAETQFQQALRLNPDSTVAARNLGLVQSRLQSVPK"
BASE COUNT          578 a          596 c          599 g          523 t
ORIGIN      
        1 catccacaat ttgccgcgct cgatggtaaa ttccaggtcc tgcacatcct tataatgccg
       61 ctccagggtt tcggcgactt ttgcaaattc ggcatacacc tcgggcattt catcctggag
      121 atgggcaatc ttcgccggag tccggatgcc tgccaccaca tcttcaccct gggcattggt
      181 caggtattcg ccaaacagca gccgctcacc cgtcgaagga ttgcgggtaa aggccacgcc
      241 cgttcctgaa tcctggccca tgttgccaaa gaccatcgcc acaatattga cggcggtgcc
      301 caggtcgtct ggaattttgt tgacgcggcg atagtccacc gcccgtttgc caaaccagga
      361 cgagaacacg gcctggatcg agagttcaag ttgtttatag ggatcgctgg ggaaagactg
      421 gccggtttct ttaaacacca ctgactggaa tttgacgaca atttctttca agtcatcagc
      481 ggtcagatcc gtgtcaagat gaacgccttt gtggtgcttg gcatgctcga aaatgtggtc
      541 gaatttttca cccggaatcc caagcacgat gcgcccaaac atctggataa agcgacgata
      601 ggcgtcctgg gcaaatcgtt cgttggaggt taattttgcc agcccctgaa ctgtcacatc
      661 attgagaccc aggttcaaca cggtatccat catgcccggc atcgaaaatt tcgcaccgga
      721 tcgaaccgaa accagtaacg ggttttcagg attgccaaac cctttccccg tcgtctgttc
      781 aaccgttttc atggcggcca ggacttcatc ccacaacccg gcgggaaaca ccttccctgc
      841 cgagtaatat tcattgcagg aatgcgtggt gatggtaaat cctggcggaa ccggcaagcc
      901 ggccagcatc atttcagcca gacctgcacc tttaccaccc aacagatctt tcatttggcc
      961 gttgccgtcg gcagtgccat ttgcaaagag ataaactcgc tttgttgatt tttcagccat
     1021 tggtatctcc aaacttgaat gtagtgaata gtcagtagtc agtcgtttgt ggaatcgagc
     1081 acagaatgca aagcgaaggg atgaaggggc gatgtacgaa actatgaggt atgagctatg
     1141 aggcggaaat agagctattt cactgattca aaatcatctg tctatcataa gtcatacgtc
     1201 ataattcatc ctttccttaa tgaagaccga ctaaagtgaa aactctcgca attgcttgag
     1261 cacggtgtag gctttcaatt cgcgctctaa cgaagtccga atcagatgct ggtttaattc
     1321 ttccaggaca cgtcccgtct ggggttcaat gatgacggtg gcaaattctg cgggaggaca
     1381 tcgcaaaata cgcccgatga ggtgacgtga agccggtgaa agaaccaacc cgcgccgctc
     1441 gctgcaactg gcgcactccg gtgtgccgtc gcgtgccaga tacaatgatt cctggctggg
     1501 aaagttagtc ccgcaggtca cacacacaga taaatcagga taaaagccag agagcttgag
     1561 cagccaggtt tcaaagtatc ggagcaaagc gtcagtggcg tcaagttcag actcaacaca
     1621 gggcaacagt gcggccagga gccggtagat gtgatcattg ggctggtggg cgggtaaaaa
     1681 ctcggtaagc aactcgctcc agtagctcaa aattgccgcg atttccgggt tggctgccgc
     1741 ctcaaaatga gatttcacca gttcacaatc ggccagttcg accaggtcac gaccttcgcg
     1801 ctcgcgaaat ttgaggtgaa cctgtgaaaa gagttccagc ccggcgccat atttgctctt
     1861 gagccgccgc gaaccatgag cgacagcccg aacgacgccg acttgacggg taaagaagac
     1921 acagactttg tgatgttctg agaaagggta ggtccgcagg gcaaaggctt cagtttcaat
     1981 ggtcggcata acacatgacc tggaggttat ttggggacag attggagtct ggattgtacc
     2041 agccccaggt tacgagcggc gactgtcgaa tccgggttga gtcggagggc ctgttgaaac
     2101 tgggtttcgg cctcggtcag ttttcccaac cgcagcaaaa ccacaccata attgttaaag
     2161 ccacgatcaa aatctggata ctggcgaatc aacgcttgat agagtctcaa ggcagcatcc
     2221 atttgattgg cctgcgcccg aatatcagcc gccaggagct gggcatccgg aataactgga
     2281 ctgaggacaa ctgccc
//
LOCUS       DAOQDO010000981         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43075_length_2295_cov_2.775446, whole genome shotgun sequence.
ACCESSION   DAOQDO010000981 DAOQDO010000000
VERSION     DAOQDO010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43075_length_2295_cov_2.775446"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="PKZ53_14995"
     CDS             <1..1562
                     /locus_tag="PKZ53_14995"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ41796.1"
                     /translation="AGSSAKVGEIANADVVFIKGGDQGVYYDEWNNTLLETHIRTVIG
                     RGGAIGGTSAGAMSQAEYCFSGGYDTISADVLADAKSTMLDDRYTPGTSGIHTDFLSF
                     VPGCVIETHYTERGRLGRAVGILAKAIEDTNNHTIYAIALDSYTGVWIRNGVAEVVGE
                     GEVSFIKESATSTLRRTAGKPLFYTNLILDRLTHGWKYNLTTRTPVTTSVPAGVSAVT
                     YAGDGSANSGVLSIAGATETDKSKFQKQATYYPTNYGVTTTTATTYVRNAIGFMDAGN
                     STNRADKQETLYMALHDNPSYIGFLAFSGGTISRTSTAPDELSFGGTLATIVIDGKTV
                     TYKGLSPYVSGYATTGGALRAAALINATLHILSDSTTQNTRLNSRTHALVTGTGGGGG
                     GGTGTSINEVEPNNSRTAPQDITSLAFPVTVTGKIGTSSDRDYFRFTLGSGQSVTINL
                     TVPSNKDYDLYLLSNSGVTEARSTKAGNGVAESITFTNTSSGTNTYFAEVEGYSGAFS
                     TTASYTLRIAK"
     gene            complement(1605..2144)
                     /locus_tag="PKZ53_15000"
     CDS             complement(1605..2144)
                     /locus_tag="PKZ53_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41797.1"
                     /translation="MYIEFVPVLFFFWLALTIICLTIGAGMAAEKFPKLRTHLLVGPV
                     TVLVSFLIGTTVWFEFSAWQSRRELRVYIQSGGAQAPSLFFPFRARCYESVRGIPYAM
                     YGTAAATGYNDPDPQVRARCLKASLALFDGEGSDLYGPIVKVLHQAQQDPAPEIQQIL
                     VDSGIKKPVGGSQPAGKEN"
BASE COUNT          602 a          649 c          555 g          489 t
ORIGIN      
        1 atgccggctc gtcagccaaa gtcggcgaga ttgccaatgc cgatgtggtt tttatcaaag
       61 gtggcgacca gggcgtatac tatgacgaat ggaacaatac actgctcgaa acacacatcc
      121 gcaccgtcat cggacgtggc ggcgcgattg gtggtaccag cgccggagcg atgagtcagg
      181 ctgaatattg ctttagcggc ggctatgaca caatctcagc cgatgtcctg gccgatgcca
      241 aatcaacgat gctggatgat cggtacaccc ccggcacgtc gggaatccac actgactttc
      301 tgtcttttgt gccaggctgc gtgattgaaa cccactacac cgagcgcgga cggcttggcc
      361 gggcagtcgg aattttggcc aaagcgattg aagacaccaa caaccacacc atttatgcga
      421 ttgcactcga ttcctacact ggcgtctgga ttcgcaatgg tgtggccgaa gtggttggcg
      481 aaggcgaagt ttccttcatt aaagaatcag ccacttcaac cctgcggcgg acagccggca
      541 agcccttgtt ctataccaac ctgatattgg atcgcctgac gcacggatgg aaatacaacc
      601 tcaccacccg cacgccagtc acaacgtcag tccccgctgg ggtgtcagcc gtgacctatg
      661 ctggtgacgg cagtgccaat tccggagtgc tttcgattgc cggcgccacc gaaacagaca
      721 aatccaagtt ccagaaacag gccacctatt acccaaccaa ctatggcgta accacaacca
      781 cagcaaccac atatgtccgc aatgcgattg ggtttatgga tgcaggcaac agcaccaacc
      841 gggccgataa acaggaaacg ctctatatgg ccctgcacga caacccgtca tacatcggat
      901 ttttggcctt ctcaggtggc accatcagcc ggacgtcaac cgcacctgat gaactttcct
      961 ttggtggaac gctggccacg attgtgattg atgggaaaac agtgacctac aaaggcttat
     1021 caccatacgt ctcaggctat gcgaccaccg gcggagcact tcgggccgca gccttgatca
     1081 atgccacact gcatattttg tctgattcaa ccacccaaaa cacccggctc aacagccgaa
     1141 cccacgcgct ggtcactggc actggcggtg gtggtggcgg tggaaccggc accagtatca
     1201 atgaagtcga acccaacaat tctcgcaccg cgccacaaga catcaccagt ctggccttcc
     1261 ccgtcacagt taccgggaaa attggcacca gcagtgatcg tgactacttc cgctttaccc
     1321 tgggttctgg tcagtcagtg acaattaatc tgacagttcc gtcaaataaa gattatgacc
     1381 tgtatttgct gagcaacagt ggcgttacag aagcccgctc aaccaaagct ggaaatggtg
     1441 tggccgaatc aatcacgttc actaacacca gcagcggcac aaacacctat tttgctgaag
     1501 tcgaaggcta tagcggtgca tttagcacga cggccagtta cacgctgaga atcgcaaaat
     1561 agcgaagtag ccaaaactcc tcttttcatt gaaggactct tccgttaatt ctctttacct
     1621 gccggttggg aacccccaac cggttttttt atgcctgagt ccaccaaaat ctgctgaatt
     1681 tccggagctg gatcctgctg cgcctggtgt aaaactttga caatgggacc ataaagatca
     1741 gagccttccc catcaaaaag cgccagactg gcctttaagc atcgggcacg aacctgcggg
     1801 tcaggatcgt tgtatccggt cgcagccgcc gtgccataca tggcatatgg gatcccccga
     1861 actgattcat aacatctcgc tcgaaatgga aaaaacagcg acggcgcctg ggcaccacca
     1921 gattggatgt aaacccggag ttcccgacgt gattgccagg ccgaaaactc aaaccacacc
     1981 gttgtcccaa taaggaatga caccagaacc gtcacgggtc caaccagcaa gtgggttcgg
     2041 agtttcggga atttttcagc cgccattcca gctccgatgg tcaaacagat gatggtcagg
     2101 gccaaccaga aaaagaaaag aactggaaca aactcaatgt acatgacctg ttcccctcag
     2161 aagtcagatt gaacgatgtt tctggttgga cacatttcct gccgcaaagt tccggcttta
     2221 ttcctggaaa acagtcaagt aattcaatta actgagctta gcaaactact gactactgac
     2281 tactggctac tgact
//
LOCUS       DAOQDO010000982         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43103_length_2294_cov_3.082180, whole genome shotgun sequence.
ACCESSION   DAOQDO010000982 DAOQDO010000000
VERSION     DAOQDO010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43103_length_2294_cov_3.082180"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..>2294
                     /locus_tag="PKZ53_15005"
     CDS             479..>2294
                     /locus_tag="PKZ53_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41798.1"
                     /translation="MSLESRTSILDWNTAQSIGKEIIVGNTFAFLTPKTWEHPTLGWI
                     ARLLTGSGIVPPRIVSLAGTHAVQIGGAEFKPFTLPELNLATVTCDKAIYRTEHDTVN
                     LLALNPLAPRSSANLVISADGREFSRHAVQLGDHGEATFQLRDLPVGAYEAAFDGTTA
                     DTAGCSFTVAEYRLSPLTAAFHHKKFSGDNQFSFVLNLESFGTPVEGDIALELMEPNR
                     RLAKITVRAEQGKVKGSFSLTGNGPHTINIQLVADPGKTATLPIVGSRASERQLTTFS
                     RLGTEISGTLLPTEDSRLVRGIFLEEGAHSTTPFSLERVDTYRARLTVLGRVQSLTVL
                     VIDPTFPKPGPDAVNPETAPYPGATDEPYQRGEALFKDGYFSDSAAIFEQARAQCEKP
                     HPFYAYFAACGYARQGQKARAVAALRQSIEDGWTDFHHLATDEDLKSLQGYEPYEWLK
                     ARGIREFTYAEITRTTTLEFEMPGPVGLLAIGAFVNEKPWEGWAATLAPVELAPEILI
                     PLQCEPGTDITIHVTTETLNPSALYMIVKDARLLSGDTPLSRLAGGIKAFVEATGKEL
                     SVGTPEKTLAHVVQQAYGIEKVYRGGGPGSFPPPVMSQA"
BASE COUNT          530 a          635 c          601 g          528 t
ORIGIN      
        1 gagcgtctac gtcacggggt ttccgattga gccgagcggc aaccagttcg atagcgtcag
       61 tgaaatcagg aacgtgtcca agcctgagac tttgggacat tcccaacagg tcgaaccggg
      121 caaatccggc tggatagcgg ctttcagaag cgaggagtcg ttccattctc tcaacacaaa
      181 tttttcgcga cattccactc attgtttctt cttctcgatg cactggtcac cggacaacaa
      241 ttcgggcgat tttgggttat aaacggctac catcacacgc tcctgattca tcttgcgaac
      301 gtctgattgt ggttttagca cactcacatc ggaactgaat agcaaccaca ttcccaaaag
      361 aacttgtggc caaatcctgg cccggtgaac cataatctcg ccagcattcc cgttggtaca
      421 ttccattcat aagctgactt ttccacaatc tcattttttt gctggaggta tggccgctat
      481 gtcgcttgaa agccgcacat caattctgga ttggaacact gcccagtcca ttggaaagga
      541 aatcatcgtc ggaaatacct ttgcctttct gaccccaaag acctgggaac acccgacact
      601 cgggtggatt gccaggctcc tgacgggcag tgggattgtg ccaccacgga ttgtttcact
      661 ggctggaacc catgcggttc agattggcgg tgctgaattc aagcccttta cgctgccgga
      721 gttgaatctg gcgacggtga cctgtgacaa agcgatttac cgaaccgaac acgataccgt
      781 caatttactg gccctcaacc cgctggcgcc acggtcgtcg gccaatctgg tgatttcggc
      841 agacgggcgt gaattttccc ggcatgcggt tcaactgggt gaccacggag aggccacctt
      901 tcaattgcgc gaccttccgg ttggagccta tgaagccgcc tttgacggaa ccaccgctga
      961 caccgccggg tgttcgttca ccgtggctga ataccggctg tcgccgctca ccgccgcctt
     1021 tcaccacaag aagttttcgg gcgacaatca attttcattt gtgttgaacc tggaaagctt
     1081 tgggacacca gtcgaaggtg acattgccct tgaattgatg gagccaaacc ggcgattggc
     1141 caaaatcacc gtccgggctg aacaaggcaa agtcaaagga tcgttttcgc tcactggtaa
     1201 tggtccacac acgatcaaca tccaactggt tgccgaccca ggcaaaaccg cgacgcttcc
     1261 aattgtcgga agtcgtgcca gcgaacgcca gttaaccacg ttttccaggc ttgggaccga
     1321 aatcagcggc acgttgctgc ccactgaaga ttcacgactg gtgcgaggga tttttctcga
     1381 agaaggtgct cattcaacca ctccgttttc gcttgagcgc gttgatacct accgtgcccg
     1441 cctgacggtt ctggggcgag tccagtccct gacggtgctg gtgattgacc cgacatttcc
     1501 caaacctgga cccgatgccg tcaacccgga aacggcaccg tatccgggtg ccaccgacga
     1561 gccttatcaa cgtggcgaag cgctgttcaa agatgggtat ttttctgatt cggcggctat
     1621 ttttgaacag gcacgggccc aatgtgaaaa accgcatccg ttttatgcct actttgccgc
     1681 ttgcggttat gcccgacaag gacaaaaggc ccgtgcggtt gcggcattgc ggcaatcaat
     1741 cgaagacggc tggactgatt ttcaccatct ggcaacggac gaagatctga aatcacttca
     1801 agggtatgaa ccctatgaat ggctcaaggc gcggggcatt cgcgagttca cctatgcgga
     1861 aattacccgg acgaccacgc tggaatttga aatgccggga ccggtcggcc tgctggccat
     1921 tggggcgttt gtgaatgaaa aaccctggga aggctgggcg gccaccctgg caccggttga
     1981 actggctcca gaaattctca ttccattgca gtgcgaacca ggaacggata tcacaattca
     2041 cgtcaccacg gaaacgctca accccagtgc gctctatatg attgtcaagg atgctcgcct
     2101 gttaagtggg gacacaccgc tgagtcggct ggccggtggc attaaagcgt ttgttgaagc
     2161 aaccgggaaa gaactttcgg tcggaacacc tgagaaaaca ctggctcatg tggtccaaca
     2221 agcctatgga attgaaaaag tgtatcgtgg tggaggccct ggttcctttc ccccacccgt
     2281 tatgtcccag gccc
//
LOCUS       DAOQDO010000983         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43149_length_2293_cov_3.563003, whole genome shotgun sequence.
ACCESSION   DAOQDO010000983 DAOQDO010000000
VERSION     DAOQDO010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43149_length_2293_cov_3.563003"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..>2293
                     /locus_tag="PKZ53_15010"
     CDS             156..>2293
                     /locus_tag="PKZ53_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41799.1"
                     /translation="MTESKEPDPLIGMVLDGKYRLDRILGIGGMATVYAATRLQFGDT
                     VAIKVLSAEAMQQHQAVKRFEREARAVASIKHPNVISVYDFGALPDERAYMVMEYIRG
                     ESLREELKANGRLTSERAVQVMRAVCAAVQAAHEEGVIHRDLKPENVMLARHRDGTEI
                     VKVVDFGVAELHESAAASAMTKLTEVGLMVGTPHYMSPEQCRSETLDKRSDVYSLGII
                     LYELLTGTVPFNARTVSAVIIQHATEPPRRLRDLSPDIPWSLEQVVLRALEKDRSRRP
                     QSAAELSQNLQMAFDSLQAQTNQTLISQQPPTRELRAPTTFPTLPPVSFETEVRDTPP
                     RPLRRGLTGYLRVPGGTTDSGFVRRPTGFRRTASLHFDSLTGIYNTTYLSQRIDETLV
                     ESKKVTVLMLGIDQFGQINNSYGYRAGDLLLRELASWLRDQVGDQGIVSRAGSDEFLI
                     LVADQAIEETLGLAQALQQEITSHVFLKSEVSEGVPLKVTIAVVTSPEDGTTPQDLLD
                     AASRALSQAKQSHHGGVYRLGEEGDDRSTARLNFNAFVGRARELRQLSDAFDRTMRGR
                     TKPVLIQGPTGLGKTRLTKEFRRRMAGKEVTFLSGAFFEAGQGAPYKTFYDSLRGPIQ
                     ALLEVENGQQAEAVFGTLTDRVFQDFGSDEGFMYFCDLVQVENAHEKYLTFEYLTNLF
                     LILAQQKPVVLFLDDLHWSDELSFELLAYLA"
BASE COUNT          527 a          581 c          654 g          531 t
ORIGIN      
        1 ctgcaaacaa cgttcctgaa gacggaagag gtctgtcatc tgacctgttt cgggctcagg
       61 gattcaccgc acacagtgaa agaggaggtt cggcgcacct catcaaatca tttttgatga
      121 gggatcccct cggtattttg cgctgaaaac gcttcatgac tgagtcaaaa gagccagatc
      181 cgttaattgg aatggtgttg gatggcaaat atcggctgga ccgcattttg gggattggcg
      241 gtatggcaac agtctatgct gccacccggc tccaattcgg ggacactgtc gctatcaaag
      301 tcctcagtgc tgaggcgatg cagcaacatc aggccgtcaa gcgatttgaa cgcgaagccc
      361 gtgcagtggc cagcattaag cacccaaatg tgatttcagt gtatgatttt ggcgcccttc
      421 cggatgaacg ggcctatatg gtcatggagt acattcgggg cgaatctctg cgcgaagaac
      481 tgaaagccaa cggacgatta acctcagaac gagcggtaca ggtgatgcgg gcggtctgtg
      541 cggcggtcca ggctgcccac gaggaaggcg tgattcatcg cgaccttaaa cccgaaaacg
      601 ttatgcttgc ccgccatcgg gatggcactg agattgtcaa agtggtggat ttcggagtgg
      661 ccgaactgca tgagtcggcg gcggcttcgg cgatgaccaa gctgaccgaa gttggcttga
      721 tggtgggcac tccgcactat atgtcacctg agcagtgtcg gagtgaaaca cttgataaac
      781 gctcggatgt ctatagttta gggattattc tctatgaact gttgactggc accgttccct
      841 ttaatgcgcg gactgtttct gctgtgatca tccagcatgc gaccgagccg ccccgtcggc
      901 ttcgtgattt aagccccgac attccctggt ccttagagca ggttgtgctg cgggcgttgg
      961 aaaaagaccg gtcacgtcgc ccccaatcag ccgcggaact gtcacaaaac ctccagatgg
     1021 cctttgatag tctgcaggcc cagaccaatc agaccctgat cagccagcaa ccaccaaccc
     1081 gcgaattgcg ggcgccaacc acgtttccca ccctgccgcc ggtgtcattt gagactgaag
     1141 ttcgagatac ccctccgcgc ccgttgcggc gtgggctcac cgggtatttg cgggtacctg
     1201 gcgggaccac tgattccggg tttgtgcgcc gcccgactgg ttttcgccga actgcctcac
     1261 tgcattttga tagtctgacc gggatctata acaccaccta tctgtcacag cgaatagatg
     1321 aaacactggt tgaatcaaaa aaagtaaccg tgttgatgct gggcattgac cagtttggac
     1381 agatcaacaa ttcatatggc tatcgagccg gggatttgct gctcagagaa ctggcaagct
     1441 ggttgcgtga ccaggttggt gatcaaggca tcgtgagccg ggccgggagc gacgaatttt
     1501 tgattctggt ggctgatcag gcaattgaag aaacgcttgg gttggcccag gcactccagc
     1561 aggaaatcac cagccatgta tttctcaagt cagaggtcag cgaaggagtt ccattaaagg
     1621 taaccattgc cgtggtgact tcgcctgagg atggcaccac accccaggat ttgctggatg
     1681 ctgccagccg ggcactgagt caggctaaac aatcacatca cggaggcgtc taccgcctgg
     1741 gtgaagaagg cgatgatcgc tcgactgccc gcttgaactt taatgccttt gtcgggcggg
     1801 cacgggaact gcgccagtta agtgatgctt ttgaccgaac catgcgcggt cgaaccaaac
     1861 cggtgctgat tcagggaccg accgggcttg ggaaaacccg actcaccaaa gaatttcgac
     1921 ggcggatggc cggcaaagaa gtgacatttt tgtcaggcgc cttttttgaa gctggccagg
     1981 gggcacccta taaaacattt tatgacagcc tgcgcgggcc aattcaggca ttgcttgaag
     2041 ttgagaatgg ccagcaggct gaagccgtgt ttggaacgct gacagatcgg gtctttcagg
     2101 attttggaag tgacgaaggg tttatgtatt tctgtgacct ggtgcaggtt gaaaacgccc
     2161 acgagaaata cctgaccttc gagtacctga cgaatctgtt tctcatcctg gcccagcaaa
     2221 agccagtggt cttatttttg gatgatctgc actggtctga tgaactgagt tttgaattgc
     2281 tcgcgtatct ggc
//
LOCUS       DAOQDO010000984         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43156_length_2293_cov_3.142091, whole genome shotgun sequence.
ACCESSION   DAOQDO010000984 DAOQDO010000000
VERSION     DAOQDO010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43156_length_2293_cov_3.142091"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2194
                     /locus_tag="PKZ53_15015"
     CDS             119..2194
                     /locus_tag="PKZ53_15015"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ41800.1"
                     /translation="MVKEVVSHAFDHLMERESVVFQKDIEKHALQNYAFAGQVRLSDI
                     RNELEHRVRAGELLCRPNNEKSEMVYTTRELQQLELDTIHLIQPGTRNSQAIASSLGE
                     LASAKTMADDQGRMLNLDQQRVFEYLGTAGDQFVSLHGKAGVGKSLVLKRLADLAERN
                     GYLVRAFAPTLSATETLRTSGLPNPETVQKHIRSQSEPPTHQPQIWLVDEASLIGLRD
                     MHALLTKAKSERARVILSGDTRQFSSVPAGRSYDQLLRHGLPNVELNRITRQVEARQE
                     IRDAVEALSEGKIRQGLEKLRGIEDDRGNASIMIEPDQHHRHKRIADLYLSLSGENLV
                     ICPTNRERNAVNRAIREARIEKGEVGKNGFVATVYINQNVTSAARKEARSYHLGNVIR
                     FAKVRHEELEARQWYTVVGRNLTQNTITVADQVGHEITYHPSKHYGIEDIFRVESREF
                     SAGDRIQFRGTDRDRGLRSGDLAQIVAIDQRGRAVLATKTDQTRVVDFSEYHTVDYAY
                     VSTGHGAQGRTVDNVIQLADGEHRESVANTKAQYVAGTRERKGLYIVTDDYDAMVRQA
                     ERQYEKMVALDLTPPLVPVPNKSHNPAHAPLPNAKKQEFSPEGRQELMVKAWEERQIE
                     VEFGLPHDGSNQSFQIIFGAAWKNNQPACWDEITTVMNRRETSHQQVIQPASPTERQE
                     RTPHHGFSV"
BASE COUNT          612 a          574 c          639 g          468 t
ORIGIN      
        1 cgccgggtgg aaattgcgct atgaaacgtt tgggtttgat gtggctgcct atgtcagcca
       61 gacccggcac taagctaaca aaaacaaatt ttcctcactg aatcagaacc atcaggagat
      121 ggtcaaggaa gttgtcagcc atgcttttga tcatttgatg gagcgggaaa gtgtggtttt
      181 ccagaaagat attgaaaagc acgcgttaca aaactatgcc tttgccggtc aggtgaggct
      241 ctccgatatt cggaatgaac tggaacacag agttagagcc ggcgagcttc tgtgccgacc
      301 caataacgaa aaaagtgaga tggtgtatac cactcgtgaa ctccaacaat tggaactgga
      361 tacgattcac ctgatacagc cgggaacgag aaacagtcag gcgattgctt ccagtttggg
      421 agagcttgcg tcagccaaaa caatggcgga tgaccagggg cggatgctca atcttgatca
      481 acagcgggtt tttgagtatc tgggaacagc gggtgatcag tttgtttcgt tgcacgggaa
      541 agccggtgtc gggaaaagtc tggttttaaa acgattggcg gatctggccg agcgaaacgg
      601 gtatctggtg cgtgcctttg caccaaccct ttccgctacg gaaacactcc gaaccagtgg
      661 tttgccaaat ccggaaacgg ttcaaaaaca tattcggagc cagtcagagc caccaaccca
      721 tcaaccgcaa atctggctgg tggatgaagc cagcctgatt gggcttcggg atatgcacgc
      781 tttgctcacc aaagcaaaat ctgagagggc gcgggtcatt ttgtctggcg atacgcgaca
      841 gttttcaagc gttccagccg ggcgcagcta cgaccagctc ctcaggcatg ggttgcccaa
      901 tgttgaactc aaccgaatca cccgtcaggt tgaagcccgc caggaaatcc gtgatgcggt
      961 tgaagccctg tcagaaggca aaatccgcca gggattggaa aaactgcgtg gcatcgagga
     1021 tgaccgtggc aatgccagca ttatgattga gcctgatcaa caccatcgcc acaaacgaat
     1081 tgccgacctg tacctgtctc tgtccggtga aaacctggtg atttgcccaa ccaaccgcga
     1141 acgaaatgcc gtcaatcgag ccattcggga agctcggatc gagaaaggtg aagttggaaa
     1201 aaacggcttt gtggcgactg tgtatatcaa ccaaaatgtc accagtgcgg cccggaaaga
     1261 agcccgcagc tatcacctgg ggaatgtcat ccggtttgcc aaagttcgcc acgaagagtt
     1321 ggaagccagg cagtggtaca ccgtggtggg tcgcaatctg actcaaaaca cgattaccgt
     1381 ggctgaccag gtaggccatg aaatcaccta tcacccgagt aaacactatg ggattgaaga
     1441 cattttcagg gtcgaatcac gcgagttttc agccggtgat cggatacagt tccgggggac
     1501 tgatcgggat cgggggctgc ggagtggtga tttggctcag atcgtcgcca ttgatcagcg
     1561 gggacgggcc gtcctggcca ccaaaactga tcaaaccaga gtggttgatt tcagtgaata
     1621 tcatacggtt gactatgcct atgtgagcac cggccatgga gcgcaaggcc gaaccgtgga
     1681 caatgtgatt caactggctg atggcgagca ccgcgaatcg gtcgcaaata caaaagcgca
     1741 gtatgttgcc gggacacgcg agcgaaaagg gctttatatc gttacggatg actatgacgc
     1801 gatggtccgc caggccgagc gccagtatga aaagatggtg gcgctcgatc tgaccccgcc
     1861 acttgttccg gttccgaaca aaagtcacaa cccagctcac gcccctcttc ccaacgcaaa
     1921 aaaacaggag ttctcacctg aagggcgaca ggaattgatg gtgaaagcct gggaggaacg
     1981 acaaatcgag gtggaatttg ggctgccgca tgacggaagc aatcaaagtt tccagatcat
     2041 ttttggagcg gcctggaaga acaatcagcc agcttgctgg gacgagatca ccaccgtgat
     2101 gaaccgccgt gaaaccagcc atcagcaggt gatccaaccc gcgtcaccaa ccgagcgcca
     2161 ggagcgcaca cctcatcatg gattttcagt gtaaggcagt gacgctacct gagtgggtcg
     2221 aataccttgc ctgaccgacc actgaatccg gttgagacca accgcccata aaatcacagc
     2281 caggctgata ccg
//
LOCUS       DAOQDO010000985         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43157_length_2293_cov_2.982127, whole genome shotgun sequence.
ACCESSION   DAOQDO010000985 DAOQDO010000000
VERSION     DAOQDO010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43157_length_2293_cov_2.982127"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..661
                     /locus_tag="PKZ53_15020"
     CDS             83..661
                     /locus_tag="PKZ53_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNJ41801.1"
                     /translation="MPHFVLPDFVHHALPEKATRLCETLNAPSRLVAHLTVVHDVACQ
                     IVLAFPHLFPDAHFDQEQVLFGAATHDLGKARFPQELQQSGELHENHGPDLLISLGVS
                     SELARFARTHAAWKQTETRALLDFEDLLVGLADNWWKGKRPPELEEAILHHLATQTQT
                     EAWELFTRLDELAQTITHPAQERLDWMRSFPA"
     gene            803..>2293
                     /locus_tag="PKZ53_15025"
     CDS             803..>2293
                     /locus_tag="PKZ53_15025"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41802.1"
                     /translation="MSDLQRYVLTLIMVALVTGSEPPALVRNLTVPSVPLVQTRQSGL
                     DTSRVDGKIVTGHPQTLPIQMKKSEFASVVVGQRGVDLHVTVTDPTGQKVVEVVCPSL
                     TQSRLPVVWLSKLPGKYLIEIQLADPKADSTPFTFQRVELRPTRPTDLALIQAQADFY
                     EAVSHFKQSQFEEALLLVISALSTREKLSGPDTLDVADCFNLLGNVTYYQKKQDLAET
                     WYARSLALREKQLPDDDPDLATVRYNLAWVWHQQKQLSKAEAMFRAAISTYEKGLGIN
                     QGEALKCYEKLGEIYFTQRDVTRLAAVEKELTDRIDQVAPTEASKLASSIFTIGGLYL
                     QGGNYPKSIQIQERARSLLENLPAPNKEIQADVYTELGKGYFFSNDLVKAETYLAKAL
                     AVREQYTATDEPNIAAALSNLALVYRARGDLNRAEPLLLRAVSINEKIYGTEHEYVGA
                     SLSTLGLLYISKENFRAAEPLFQRALAMFEKVLPPDHPNIPFVLNNL"
BASE COUNT          596 a          625 c          529 g          543 t
ORIGIN      
        1 ccttttgcga tagtcaaatg gttcaatcaa ataaactgag tgaactactg acttcaaact
       61 ggtgtaatct ctgagtcctt ttatgcccca cttcgttttg ccagactttg ttcatcacgc
      121 gctgcctgaa aaagcgaccc ggctttgcga aacgctcaac gctccatctc ggctggtggc
      181 tcatttgaca gtggtccacg acgtggcctg ccagatagtg cttgcttttc cacacttgtt
      241 tccagacgca cactttgacc aggagcaggt tttgtttggc gcggcgactc atgatttggg
      301 gaaagcacgc tttccccagg aacttcagca atcaggcgag ttacacgaaa accacggccc
      361 agatctcctt atttctctgg gggtgtcatc cgaactggcc cggtttgccc ggacccacgc
      421 tgcctggaaa caaaccgaaa cccgtgctct tctggacttt gaagaccttc tggttgggct
      481 tgcagataac tggtggaagg gcaagcggcc acccgaactt gaagaagcca ttctgcatca
      541 cctggcgacc caaacccaaa ccgaagcctg ggaactgttt actcggcttg acgaactggc
      601 ccagaccatc acccacccag cccaggagcg actcgactgg atgcgctcat ttccagctta
      661 accaccaatt tcaagctcca aagaaaccgc acccaatcag tgccggtcac tggaaaattc
      721 acccaaaaag catcaaactc caaaaactaa tccggcaatc ctttcagccc atctcattct
      781 cctcaattgg agtttcatac ctatgtcaga tcttcaacgg tatgttctga ccctgatcat
      841 ggtcgccctg gtcaccgggt cagaaccacc ggctctggtt cgcaacctga cagttcccag
      901 tgtgccttta gttcaaacac gccaatctgg attggacaca agcagagttg atggaaaaat
      961 tgtcacgggt catcctcaga cattgccaat tcaaatgaaa aagtctgagt ttgcctcggt
     1021 tgtggtgggt caacgagggg ttgatctgca cgtcacagtg accgatccaa ccggacaaaa
     1081 agtggttgag gtcgtctgcc cgagcctgac ccaaagccgc ctgccagtag tatggttaag
     1141 caaattaccc ggaaaatatc tgattgaaat tcaactggct gacccgaaag ctgattcaac
     1201 tccattcacg ttccaacggg tcgaacttcg gccaactcgc cccaccgatc tcgccctcat
     1261 tcaggctcag gctgattttt atgaagcggt ttcccatttc aaacaatcac agtttgaaga
     1321 agcactgttg cttgtcatct cagccctttc aaccagagaa aaactttcag gtccagatac
     1381 ccttgatgtg gctgattgtt tcaatctact gggaaatgtg acctattacc aaaagaaaca
     1441 agatctggct gaaacctggt atgcccggtc actggcactc agagaaaaac aattgccaga
     1501 cgatgatccc gatctggcca ccgttcgata taacctggcc tgggtctggc accaacagaa
     1561 gcaactctca aaagctgaag cgatgtttcg agcggcgatt tcaacgtatg aaaagggact
     1621 gggaatcaac cagggcgagg ccctcaaatg ttatgaaaag cttggcgaaa tttacttcac
     1681 ccaacgtgat gtcacccggc tggcggcagt ggaaaaagaa ttgactgacc gcatagacca
     1741 ggttgcccca actgaggcgt ccaaactggc ctcttccatt ttcacaattg gagggttgta
     1801 cttacagggc ggaaattatc ccaaatcaat ccagattcaa gagcgcgccc gctctctttt
     1861 ggaaaacctg ccggccccaa acaaagaaat ccaggccgat gtgtatactg aattgggtaa
     1921 agggtatttt ttcagcaacg atctggtcaa ggctgaaacc tacctggcca aagctctggc
     1981 tgtgcgggaa caatacacgg caactgatga accaaatatc gctgcggcac tcagcaatct
     2041 ggccctggtg tatcgtgccc gtggcgattt gaaccgggca gagccgttgt tactcagagc
     2101 ggtttcgatc aatgaaaaga tttacggcac tgagcacgaa tatgtgggcg cctcattgag
     2161 caccctgggg ttactttaca tttccaaaga aaatttccgg gcggctgagc cattgtttca
     2221 acgcgctttg gccatgtttg aaaaggtgct gcccccagac caccccaata ttccttttgt
     2281 actcaataat ctg
//
LOCUS       DAOQDO010000986         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43209_length_2292_cov_2.814931, whole genome shotgun sequence.
ACCESSION   DAOQDO010000986 DAOQDO010000000
VERSION     DAOQDO010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43209_length_2292_cov_2.814931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2292
                     /locus_tag="PKZ53_15030"
     CDS             <1..>2292
                     /locus_tag="PKZ53_15030"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ41803.1"
                     /translation="VTLDPTGPPTIPVYTVNYPAFKYRVYRVEPNQWKAYTSHRDWSF
                     QNRAEVPPPPGWKTISSKVVKLDPRPEELIETELDLRPWLTSGTGHLVLHVTPLLAGK
                     NPHYQNQTLLWIQSTRLAADAYRDQSDLMVSVTSLVDGKPVNQASVTLMPEKLVTRTG
                     PDGLGVLKVGPSTSIGSRILVIRQGEDSLLVPEPQFWEGDATDWDQPSIVDRFQWYVF
                     DDRRIYQPGEELHLKGWIRRIEGRKGGDIDLAREHVKKLKYVLRDPRSGDLQRGELPV
                     NAFGGFDTVLSLPKNINSGICRVEFLCTSQTAGIVPGKYLHRFRVEEFRRPDFEVSVT
                     TDLQPKILHNPTEVVTSAKIYGGGVLSHAPVTWKVATKPVSFRPPNRDEFVFGEGFGD
                     WYPVEWEDFSEEFSWIARYSQTEEGQTFSGETDTLGHHRLHIDFDRMTQPKPFLVNAE
                     ATISDVNRQAISSSTSFLVHPSSFYVGLKSNRRWKHLEDEAFKVEVIVTDLEGNLIPN
                     QPVPFKLSRETKKGWVSLPDQTVTSANLPTLVELNPTEGGKYRLIAEITDPQGRRNQT
                     FFTLWVTGGLRQVEADLEEEQVGLISDKAVYQPGETAELAIESPFVPAQALVTVQRLG
                     VIHTERFTITEPVHRYQLPILESHVPNMTVQVDLTGATTRTNRKGLPDPNLPLRPAFA
                     TGNLELVISPVTRTLTVTATPYKPEVKPGEDNVVDVQVVDSQGIPVPQSECAVVVVDE
                     AILALTSYRLIDPIKWFYVKRPAG"
BASE COUNT          558 a          598 c          602 g          534 t
ORIGIN      
        1 cgtcacgctg gatccaaccg gacctccgac gattccggtt tatacagtca actacccggc
       61 tttcaaatac agggtctatc gggttgaacc aaaccagtgg aaagcctaca cctctcatcg
      121 tgattggagt tttcaaaatc gggcggaagt ccctccacca cccggatgga aaacaatttc
      181 ctcaaaagtt gtcaaattgg atcccaggcc ggaagaactg atcgaaaccg aacttgattt
      241 gcgtccgtgg ctgaccagcg gaaccgggca tctggtgttg cacgtcacgc cattgttagc
      301 gggaaagaac ccacattacc aaaatcagac attgttgtgg attcaatcaa cccggctggc
      361 cgcagatgct taccgtgacc agtctgattt gatggtgtca gtgacgtcat tggtggacgg
      421 aaagcccgtg aatcaggcca gcgtgacctt gatgcctgaa aaacttgtca cccgaaccgg
      481 ccctgatgga ctgggtgtgt tgaaagtggg tccttcaact tcaatcggat ctcgaatttt
      541 ggttattcgc cagggtgagg acagtttgct ggtgccggaa cctcaattct gggaaggcga
      601 cgccactgat tgggaccaac cttcaatcgt tgaccggttc cagtggtatg tctttgatga
      661 tcgaaggatc tatcaacccg gagaagaact ccacctcaaa ggctggattc gccgcattga
      721 ggggcggaaa ggtggcgata ttgatctggc tcgcgagcat gtcaaaaaac tgaagtatgt
      781 cctccgagat ccaaggagcg gtgacctgca gcgaggagaa ctccccgtca atgcctttgg
      841 cgggtttgat accgtgcttt cgttaccaaa aaacatcaat tctggcattt gccgggttga
      901 gtttttgtgt acttctcaaa cggctggaat tgtgcctggc aaatacctgc accgcttccg
      961 ggttgaagaa ttccgccgac ctgattttga agtgtctgtg acgacggatc tgcaaccgaa
     1021 aattctccac aacccaactg aagtcgtgac atccgccaaa atctatggcg gcggagtgct
     1081 ctcacatgcg ccggttacct ggaaagttgc gaccaaaccc gtgtcttttc gtccacccaa
     1141 ccgggatgag tttgtctttg gcgaaggttt tggggattgg tatccagtgg aatgggagga
     1201 cttttccgag gaattttcct ggattgcacg ctattcgcaa actgaagagg gccagacctt
     1261 ttccggggaa accgacacgt tggggcatca tcgcttgcac attgatttcg accgaatgac
     1321 ccaacccaaa ccctttctgg tcaatgctga agcgaccatt tcagatgtca accggcaggc
     1381 aatttccagt tcaaccagct ttctggttca tccttcgtcg ttctatgtcg ggctgaaatc
     1441 aaaccggcga tggaagcacc ttgaagatga agcgttcaaa gtcgaagtca ttgtcaccga
     1501 ccttgaaggc aacttgatcc caaaccagcc ggtacccttc aagttgagcc gggaaaccaa
     1561 aaagggttgg gtttcgctac ctgaccaaac ggtaacttcg gccaatcttc ccacgctggt
     1621 tgaactcaac ccaactgaag gcggaaaata tcggctgatt gcagaaatta ccgatccaca
     1681 gggacggaga aaccagacct tcttcacgct ctgggtgacg ggtggccttc gtcaggttga
     1741 agctgatctt gaagaggaac aggttgggtt gatttccgac aaagcggtgt accagcccgg
     1801 agaaaccgcc gaacttgcga ttgagtcgcc gtttgttccg gctcaagctc tggtgaccgt
     1861 gcagcggttg ggcgttattc acaccgaacg gtttaccatc accgagccag tgcatcggta
     1921 ccaacttcca attctggaaa gccacgttcc caatatgacg gttcaggttg atttaaccgg
     1981 cgcgacaacg cgcacgaacc gaaagggatt acccgacccg aatttaccgc ttcgtccagc
     2041 ctttgccacc ggaaacctcg aactggtgat ttcacccgtt acccgcacct tgacggtgac
     2101 cgcgacaccg tacaaaccag aagtgaaacc tggcgaggac aacgtggttg atgtgcaggt
     2161 ggttgattca caaggcatcc cggttccaca gagcgaatgt gccgtggtgg tggttgacga
     2221 agccatcctg gcgttgacca gttaccgact gattgacccc atcaaatggt tttatgtgaa
     2281 gcgtccagcc gg
//
LOCUS       DAOQDO010000987         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43309_length_2289_cov_3.146822, whole genome shotgun sequence.
ACCESSION   DAOQDO010000987 DAOQDO010000000
VERSION     DAOQDO010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43309_length_2289_cov_3.146822"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1170)
                     /locus_tag="PKZ53_15035"
     CDS             complement(115..1170)
                     /locus_tag="PKZ53_15035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ41804.1"
                     /translation="MSTVPSASQPVALTSGSVESKIWLTVVVIGRNEGPRLVRCLESI
                     HRANLSQFGAVEVIYVDSDSTDESVSRAKQLGATVEVVHPTRPTAALGRNAGWRQGTG
                     EFVLFLDGDTILDPGFVPTALSAFDDRAVAVVWGNRREICPEASVYNRVLDLDWMAPA
                     GPSAYCGGDALMRRSVLEEINGYDENLIAGEEPEMCRRMRARGYVILHLDVPMTGHDL
                     AMTRWSQYWRRATRTGYAYAEVSNRFKGTALPLWEAESNANLKRGPLLLTLLGISLLG
                     SLVMWNPLPLLGFFLLMTALMVRTASKFGWKSRSWPTRLLYGVHSHLQQIPIFLGQLQ
                     YFRDRKNGQKRGLIEYK"
     gene            complement(1181..2233)
                     /locus_tag="PKZ53_15040"
     CDS             complement(1181..2233)
                     /locus_tag="PKZ53_15040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ41805.1"
                     /translation="MTIPLVYALHSGNLYGTERMALVTAEGLANEYPPIIFAPPGPAL
                     QEAARMGFETHAFSSAKEFAITLRPIFARHREMIFMATGVVHSLACLAWNVLYRRQVH
                     HFHLVHGGTDELLSYGRKRRLNGTGVKLVAVSEFVKSRLIAHGANPNQIVVIENFLPD
                     DQIQKAPKRPAFSRSEIHRVVVVSRVDPIKRIDLLLDAVEQNEALRRLDFRILGTGSE
                     FEQLKNRAAQSCPNVAFVGFTSAVAEELAASDLLLHLCPVEPFGLAILEAMAARLPVL
                     VPDSGGASSIIEPGISGYRFQANSAADLGQQLQRVGALTALELNSTVEQAWNRLLSHY
                     STQSGLDQYRRLIASG"
BASE COUNT          563 a          594 c          613 g          519 t
ORIGIN      
        1 ggttccgggt tccgggttcc gggttccggg ttttcgaatt tatgtccttt atgtcttttt
       61 cgtcctttat gtcctttttc caaaccctga accctaaacc ctaaaccctg aaccctattt
      121 atattcaatc aaaccgcgtt tttgaccgtt ctttcggtct cgaaaatatt gaagctggcc
      181 taagaaaatt ggaatttgtt gcagatgtga atgcacaccg tagagcagcc gggttggcca
      241 ggaacgagat ttccagccga atttcgaagc cgttcgcacc atcagcgctg tcattagcaa
      301 aaaaaacccc agcaatggca atgggttcca catcacgagc gaacccagga ggctgattcc
      361 caacaatgtc aggagcaatg gaccgcgctt caggttggcg tttgattcgg cttcccacag
      421 cggcaatgcc gttcctttaa agcggtttga aacttcggca taggcatatc cggtccgcgt
      481 ggcccgccgc cagtattgcg accagcgggt catcgccaga tcgtggcccg tcatcggcac
      541 atccagatgc aggatgacat atccccgggc ccgcattcgc cggcacattt cgggttcttc
      601 gccagcaatc aggttttcat catagccatt gatttcttca aggacgcttc ggcgcatgag
      661 ggcatcgccg ccgcaatagg ccgaaggtcc ggctggggcc atccaatcca gatcgagaac
      721 ccggttgtac accgaggctt ccgggcagat ttcccggcgg ttgccccaga cgacagcaac
      781 ggctcgatca tcaaacgccg acagtgctgt gggcacaaat ccggggtcga ggatggtgtc
      841 gccatccaaa aacaaaacaa attctccggt gccctggcgc cagcctgcat ttcttcccag
      901 ggccgccgtg ggacgagttg ggtggacaac ttcgacggtg gcgccaagtt gtttggcccg
      961 gctcacgctt tcatcggttg agtcagaatc aacataaatg acttccaccg cgccaaattg
     1021 tgacagattg gcccggtgga ttgattcaag acaccgaacc aggcgtgggc cttcatttcg
     1081 gccaatgacc accacggtca gccagatttt ggattcgact gaaccagagg tgagcgcgac
     1141 gggttgactg gcagaaggaa cagtggacat agatttgtga ttacccagag gcaatcaggc
     1201 ggcggtactg gtccagacct gattgggttg aataatggct caggagtcgg ttccaggctt
     1261 gttcaacggt tgaattcagt tccagggcgg ttagggcacc tacccgttga agttgttgac
     1321 ccagatctgc ggctgaattg gcctgaaagc gatatcctga gattccaggt tcgatgattg
     1381 aactggcgcc gcctgaatct ggaaccagaa caggaaggcg tgccgccatt gcttctaaaa
     1441 tggcgagtcc aaacggttcg actggacaca aatgtaagag caaatctgat gccgcaagtt
     1501 cctcggcgac ggcagaagta aacccgacaa atgccacatt ggggcacgat tgcgccgccc
     1561 gatttttcaa ctgctcaaat tcagagcccg tcccaaggat tcgaaagtcc agtcggcgaa
     1621 gtgcttcatt ttgctcgacg gcgtcgagga gaaggtcaat ccgtttgatc ggatctaccc
     1681 gactgaccac cacgactcga tgaatttccg accttgagaa ggctggtcgt ttcggtgctt
     1741 tctggatttg atcatcagga agaaaatttt caatcaccac aatctggttc ggatttgcgc
     1801 catgggcaat taaccgactt ttgacaaact cggaaactgc caccagtttg acaccggtgc
     1861 cgttaagtcg gcgtttgcga ccataactta aaagttcgtc agtcccgcca tgaacgaggt
     1921 ggaaatgatg aacctggcga cgatagagca cattccaggc caggcaggcc agtgaatgga
     1981 caacgcctgt cgccataaaa atcatttccc gatgccgggc aaaaatggga cgaagcgtaa
     2041 tcgcaaattc tttggctgaa gaaaacgcgt gggtttcaaa ccccattctg gctgcttcct
     2101 ggagcgctgg cccaggtggg gcaaagatga tcggcggata ctcgttggcc aggccttcag
     2161 ccgtcaccag agccattcgc tcggtgccgt acaaatttcc gctatggagt gcataaacaa
     2221 gtggaattgt cataaggaat tgagaatgaa gaatgaagaa tgaagaatga agaatgaaga
     2281 atgaagaat
//
LOCUS       DAOQDO010000988         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43359_length_2288_cov_2.335423, whole genome shotgun sequence.
ACCESSION   DAOQDO010000988 DAOQDO010000000
VERSION     DAOQDO010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43359_length_2288_cov_2.335423"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /gene="pgk"
                     /locus_tag="PKZ53_15045"
     CDS             complement(<1..180)
                     /gene="pgk"
                     /locus_tag="PKZ53_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNJ41806.1"
                     /translation="MSLSIRDLDLQGKRVFMRVDFNVPINAEGKVADDTRIRAAIPTI
                     QLAREKGGRVILASHL"
     gene            310..1113
                     /locus_tag="PKZ53_15050"
     CDS             310..1113
                     /locus_tag="PKZ53_15050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNJ41807.1"
                     /translation="MEQAISSSYLPALRPPAIFGSVRVDNVTQSETLDLIESFIRSGE
                     HHRMVVVNASKLVLAEQDLKLREILVTSDIITADGMSVVWASRCSRNFGAGLVERVTG
                     IDTMIELSQRAAQKGYRIFLLGAAPGVAQAAAQQLQTDFPGVHIVGCQDGYFPDSESV
                     HIASMIRASRSDILFVAMGSPKQEHWLATYGAQTGAAFCIGVGGSFDHIAGRVQRAPH
                     WMQKAGLEWLFRFAQEPRRLWRRYLIGNAEFVSFLIRLIRASRSTRSRM"
     gene            complement(1213..>2288)
                     /locus_tag="PKZ53_15055"
     CDS             complement(1213..>2288)
                     /locus_tag="PKZ53_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41808.1"
                     /translation="KPITWPLKIHIANADWSVSTSGNKNLVVVLGHFPIRRSSSTYCQ
                     KKEDHSNMKSKATFIGIIVIVLLGATAIGVNYWCDNHSGGSQSGASILPPVAPTEWPV
                     DIPFHPQAIVKSKVSNGTIADRYDLSVMLPKEEVLKFYQEKFKENGWIIDPSQKGPFQ
                     IWRNGNQKVGFQLFNRGKETGVAILVYVQTNKPTSIPQLEFPVDPETGLVDLPKSVPQ
                     YPRLKPKGEEKSPSGRLHYRYETADSFVQVRDFYQKAMSEQGWALKGDMSITEGDHPY
                     MNQFFNKGEVTIGIQVTSENKVTEVNYVLFEKNAQLPDMGITQKSQLNLETTPKTGNP
                     ELPPPNLSVKPAPDKPKPEVKKQ"
BASE COUNT          535 a          528 c          537 g          688 t
ORIGIN      
        1 cagatgtgat gccagaatga cccgtccacc tttctcgcgc gccagttgga tggtcggaat
       61 tgcagcccgg attcgagtgt catcggcgac tttcccttca gcgttgatcg ggacgttaaa
      121 atcaactcgc ataaacactc gttttccctg gagatcaagg tctcggattg aaagactcat
      181 atgttttgca ccttttttca gagatggatt gaaatgaagg atgtgatgaa gtatgaaaga
      241 ggctttccag gattatcaca aatgctggca cgtgccaagt taatttcttc tttcaattca
      301 ccaaattcca tggagcaagc catctcttct tcttatttgc cagccctacg cccacccgct
      361 atctttggct cggtgcgggt tgataacgtc actcagtctg aaacccttga tctaattgaa
      421 tctttcattc ggtcaggtga gcatcatcga atggttgtgg tcaatgcctc taaactggtt
      481 ttggctgaac aagatctgaa actacgggaa atcctcgtca cttctgatat catcaccgct
      541 gatggaatgt cggttgtttg ggcgtcgcga tgctcacgga attttggtgc tgggctggtc
      601 gaacgagtca cggggattga cacaatgatt gaactttccc agcgcgctgc tcaaaaaggg
      661 taccgtattt tcttacttgg ggcagccccc ggagtagccc aggccgcggc tcaacaacta
      721 caaactgact tcccaggtgt gcatatcgtt gggtgccagg atggatattt tcctgactca
      781 gaatcagtac acatcgcttc aatgattcgg gcatcccgct ctgatatttt gtttgttgcc
      841 atggggtcac ccaaacaaga gcactggctg gcgacctatg gggcacagac cggtgcggcg
      901 ttctgtattg gtgtgggtgg aagctttgat catattgccg ggcgagtaca gcgcgcccca
      961 cactggatgc aaaaggcagg gttagagtgg ctgtttcgat ttgcccagga accacgacgg
     1021 ctctggcgga ggtatctcat tggcaatgca gagtttgtat catttcttat tcgcttgatc
     1081 cgagcttcac gatccacccg atccaggatg taaatcagga acagtagttc cttctataac
     1141 caaaaaatcc cacagaaaat atctgtggga tttttagtct gggacaaatg ttcctgatga
     1201 agtcttggct ggctactgct ttttcacctc tggtttcggt ttatcaggtg ctggcttaac
     1261 cgaaagattg ggtggaggta attctgggtt cccggttttt ggggttgtct ctaagttcaa
     1321 ttgtgacttt tgggtgatcc ccatatcagg caactgagca tttttttcaa agagtacata
     1381 gttaacctct gtcactttat tttcagacgt tacctgtata ccgattgtaa cttcaccttt
     1441 gttgaaaaat tggttcatgt acggatggtc tccttcagtg atactcatat cacctttcaa
     1501 agcccagcct tgttcggaca tggccttttg gtagaaatcc ctgacctgta caaatgaatc
     1561 ggctgtttca taccgataat ggagccgacc acttggactt ttttcctcgc cttttggctt
     1621 cagcctgggg tactgaggca cggatttagg tagatccacg agtccggttt ccggatcaac
     1681 tggaaactca agttgtggga tggaagttgg cttatttgtc tggacataga caaggattgc
     1741 tactccagtt tcttttcctc tgttaaacaa ctgaaaccct actttttggt taccatttcg
     1801 ccaaatctgg aaggggcctt tctgtgacgg gtctattatc cacccatttt ctttaaactt
     1861 ctcttgataa aatttcagaa cttcttcctt tggaagcatc acagacagat catagcgatc
     1921 tgctattgtt ccatttgaaa ccttcgactt tacaattgcc tgcggatgaa acggaatatc
     1981 caccggccat tctgttgggg cgactggagg caggatcgaa gcacccgatt gagagcctcc
     2041 actatgatta tcacaccaat agttgactcc aattgccgta gctcccaaca atacaatgac
     2101 gataatgccg ataaatgttg cctttgattt catgtttgaa tgatcttcct ttttctgaca
     2161 gtaagtactc gatgatctcc tgattggaaa atggcctaat actacaacga ggtttttatt
     2221 cccgctggtt gagacgctcc aatcggcgtt tgcgatgtga atttttagcg gccaggttat
     2281 tggcttga
//
LOCUS       DAOQDO010000989         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43423_length_2286_cov_2.787539, whole genome shotgun sequence.
ACCESSION   DAOQDO010000989 DAOQDO010000000
VERSION     DAOQDO010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43423_length_2286_cov_2.787539"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..666
                     /locus_tag="PKZ53_15060"
     CDS             463..666
                     /locus_tag="PKZ53_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41809.1"
                     /translation="MSEETQKTYHAVENNGVVTAVWEMVGRRHVRTIDPRSQEGKALL
                     AHYTPAAEAQPAAENLEQMATHA"
     gene            complement(832..1230)
                     /locus_tag="PKZ53_15065"
     CDS             complement(832..1230)
                     /locus_tag="PKZ53_15065"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ41810.1"
                     /translation="MRSNQYITDEQARVHINIPILIKGKNAEGKAYAERTMAENMTRR
                     GVFIRTSQPLEIGNTLRLYSAEDASHAIAKLEVVWVREADETQSQGVGAKLLGSNRQW
                     INYLVDNSMTLESAEGEGADEDGEIDEDDE"
     gene            complement(1380..>2286)
                     /locus_tag="PKZ53_15070"
     CDS             complement(1380..>2286)
                     /locus_tag="PKZ53_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HNJ41811.1"
                     /translation="AIRQASYQVDLFDLGTGRPYEAGIFMLEDPDLIGEYNQAAKPIA
                     QQVIHEGTDLDKATLAECLAQVNDYSAKVNEWVYQITRDILDEGRIAGIVGGDHSVPF
                     GAIQAYAERFPGLGILHIDAHADLRDSYEGFTWSHASIMHNVVTRLSGVSRLVQVGIR
                     DFCEAELDLIQRSNGRIVTHFDEQLAARKFEGASWKSLIEKIIEPLPAQVYLSFDIDG
                     LDPALCPHTGTPVPGGLSFQEAVALIAALARSGRKIVGFDLNEVAPGPEGDEWDANVG
                     ARLLYKMIGYCQLSQTAVKSAPSKP"
BASE COUNT          609 a          603 c          520 g          554 t
ORIGIN      
        1 agggccaaga tttgcacgat tttattaatc ttttcaacaa gttatagaaa attggttgag
       61 gtgccaggaa ccggcccagg agtcaaatac tgagccagat atttttttcc tggtgatggt
      121 tgggtatggg aagtttggct gcaatcgaat tgaatagtgt ttgcattgaa accgtttcat
      181 catcgaaagt caaccggggt tgaatctcaa accaaactgc atttctggag tttacagcgt
      241 gcgactcatt gcttgaccat ccaaaaacca ttcactttaa aaattttctg cccgttttcc
      301 ctaaccttca accccatcac cagcggagca acgacatagt tgagcgaaaa aactgcaaat
      361 cctggccagc aaagagtgga cagcaccagc cgggcgtgcc acaatagacc caaagtcaaa
      421 ttttcatgat cgattcacta tagacccgga ggtttagctt acatgtcaga agaaacgcag
      481 aagacctatc acgccgtcga aaataatgga gtcgtgacgg cggtttggga aatggttgga
      541 cggagacacg ttcgtacaat tgaccctcgc agccaggaag gcaaagcatt gctggcccac
      601 tacacaccag ccgccgaagc ccaaccagcc gccgaaaact tggaacaaat ggccacccac
      661 gcataggttc ttcgctctac cacgtcccat caggcaggaa ctcacttcac agtgaagtga
      721 tcctgccttt tttcgtgctc actcaacacg aaaaaactcg ccatgctaat cgggcgagtt
      781 tttctgttct ctccaaccga aacaccatta cggtgtcgcc gggacgcgcg actattcgtc
      841 gtcttcatca atctcgccat cttcatcagc cccttcacct tcagccgatt caagcgtcat
      901 tgaattatca accagataat tgatccattg ccggttgctg cctaataatt tggccccaac
      961 cccctgtgat tgggtttcat cagcttctcg gacccacacc acttcaagct tggcaatggc
     1021 gtgggaggcg tcttcagccg aatagagacg taaggtgttg ccaatttcca gcggctgaga
     1081 tgtgcgaata aacaccccac ggcgggtcat attttccgcc attgtccgtt cggcatatgc
     1141 cttgccctct gcatttttcc ctttgattaa aattggaatg ttgatatgga cccgcgcttg
     1201 ttcatcagtg atatattgat ttgatcgcat acaatcctcg ctgtgggata aagcctttaa
     1261 ttacgacagg ttgtgattgg aaatcagggt gcaggacagt cttagaagcg gaagcgacgc
     1321 ggagtgtacc acgatgtccc acagtcgcaa aagaagataa taaaaacagg gttcagggtt
     1381 cagggttttg aaggagcgct cttgacggcg gtttggctaa gctggcaata cccaatcatc
     1441 ttatagagga gccgggctcc gacattggca tcccactcat ctccctctgg accaggagca
     1501 acttcattta aatcaaatcc aactattttt cgacctgagc gggcaagagc ggcaatgagg
     1561 gcaacggctt cttgaaaact taatccgcca ggaactggtg ttccggtatg cgggcacagg
     1621 gctggatcca aaccatcaat atcaaaagaa agatacacct gagcaggaag tggttcgatg
     1681 attttctcaa tcaatgactt ccacgaagca ccttcaaatt tgcgtgccgc cagttgttca
     1741 tcaaaatggg tcacaattcg cccatttgag cgctggatca gatccagttc cgcttcgcaa
     1801 aaatcacgaa ttccaacctg caccaacctc gaaacgcctg aaagtcgagt cacgacattg
     1861 tgcataatcg aggcgtgtga ccaggtaaag ccttcatagc tgtcacgcaa gtcagcgtgc
     1921 gcatcaatgt gcaaaatgcc cagacctgga aatcgctcgg catacgcctg aatcgctccg
     1981 aatggcaccg aatggtcacc acctacaatc ccagcaattc gaccttcatc cagaatgtca
     2041 cgggtgatct gatacaccca ttcgttgacc ttggcgctat agtcattgac ctgcgccaga
     2101 cactcagcca gcgtagcttt atccaaatct gtgccttcgt ggatgacctg ttgcgcaatg
     2161 ggtttggccg cctggttgta ctcgccaatc aggtctggat cctcaagcat aaagatcccg
     2221 gcttcatatg ggcgaccagt ccctaaatca aacaaatcaa cctgataact tgcctgccga
     2281 atggct
//
LOCUS       DAOQDO010000990         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43480_length_2284_cov_3.281741, whole genome shotgun sequence.
ACCESSION   DAOQDO010000990 DAOQDO010000000
VERSION     DAOQDO010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43480_length_2284_cov_3.281741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..2112)
                     /locus_tag="PKZ53_15075"
     CDS             complement(46..2112)
                     /locus_tag="PKZ53_15075"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41812.1"
                     /translation="MQCFYHSTRKSISVWGWCLLWVALFPASVWGINPDKAITQYVTK
                     TWQDELPQNSVNAIAQTADGYLWFGTYEGLARFDGVRFTVFDRRNTPELLTNHIRKLF
                     VDRQNRLWIGSMGGGLYCLENGRFTRFGVEQGLLNNNIRSLIEGPDQSLWIGTENGLN
                     RLRDGQVTGYGESQGLNGKIILSLWFDQNGTLWIGTDKGLNQLSPDGYQIVPALDERL
                     RKQIFSITSDSSGRLWAGTEAGVVLCTPNLSQIWYHPDLPRFQVFAMCRDRENSLWLG
                     VSGEGVWRVTPRGISSFSTKNGLSNDRIRMILEDREGNIWIGTGGGGVVCLSDGKFTN
                     YTVKEGLSNDSIRVVYEDQSGAMWIGTDGGGVCRYQNGTIQTFTSQDGLPADIVRSVI
                     QCRDGTLWVGTTKGCAWRKEGKFTPYILPGDFECNIICAFCEDRNGGLWIGTFEGLFF
                     LKDGSLRLYNTAQGLHDTEIRSLFQDRDGTLWVGTYGGTIDCIRNGVVTGYPLPASHK
                     QSSVWTFHEDEAGVLWIGTDAGLLRFFQGVFTQFTTSQGMFSDTIFQLLEDNQQTFWM
                     TCNRGIFSVRKSELEALSNGQLSSIQCTVYDKLDGLNSNQCNGGTQPAGWKSRDGKLW
                     FPTLRGVSMIDPTRIPRNLTPPPVVIEQALVDGQPVDLAEMMTIPHTMQQVEFTYTGL
                     SLIVQG"
BASE COUNT          571 a          637 c          544 g          532 t
ORIGIN      
        1 ttttgtcaag cgcatccttt gtaagcagaa gaatttaaga tgcgtttacc cttggacgat
       61 caggcttaac ccggtgtagg taaattccac ctgttgcatg gtatggggaa tcgtcatcat
      121 ctcggccaga tcaaccggct ggccgtccac gagtgcctgt tcaatcacca ctggcggagg
      181 tgtcaggttt cttggaatcc gggttgggtc aatcattgat acgcctctca gcgttgggaa
      241 ccacaacttt ccatctcgcg atttccaccc ggcaggttgc gtgccgccat tgcactgatt
      301 tgaatttaaa ccgtcgagtt tgtcatacac agtacattga atcgaactga gctgcccgtt
      361 ggacaatgcc tcaagctctg attttcgaac ggaaaagatt ccgcgattac acgtcatcca
      421 gaaggtttgc tggttgtcct caagaagttg gaagatcgta tcgctaaaca ttccctgact
      481 ggtggtaaac tgggtgaaga cgccttgaaa aaaccggagc agaccagcat cggtgccaat
      541 ccagagcacc ccggcctcat cctcatgaaa tgtccagact gaactctgtt tgtggctggc
      601 cgggagcgga tatccagtta cgacaccgtt gcggatacaa tctatggttc caccataggt
      661 tccaacccac agtgtcccgt cacgatcctg aaaaagtgac cgaatctcgg tatcgtgcag
      721 gccctgcgcg gtgttgtaca ggcgtaaact tccgtccttg aggaaaaaaa gaccttcaaa
      781 cgtaccaatc cacagaccgc cgtttcgatc ctcacaaaat gcacaaataa tattacattc
      841 aaaatcccca ggcagaatgt atggcgtgaa ttttccctcc ttgcgccagg cacagccttt
      901 ggttgttccg acccacagcg taccatcccg gcactggatg acagatctga caatatcagc
      961 gggtaacccg tcctgagagg taaaagtttg aatggttcca ttctggtacc gacacacgcc
     1021 accgccatca gtcccaatcc acatcgcccc agactggtct tcatacacaa cccgaatgct
     1081 gtcgttactc aatccttctt tcacggtata gttggtaaac tttccatctg acaaacaaac
     1141 gacgcctccc ccaccggttc cgatccagat attgccttcg cgatcttcaa gtatcatccg
     1201 gatgcggtcg ttggacagac catttttggt agaaaacgaa ctgatgcccc tgggcgtgac
     1261 gcgccagacg ccctccccag aaacacccag ccacagacta ttttcccgat cccggcacat
     1321 ggcaaaaacc tgaaatctgg ggagatccgg gtgataccaa atctgactca ggtttggggt
     1381 acagagcacg actccggctt cggtcccggc ccaaagcctg ccgcttgagt cactggtaat
     1441 tgaaaaaatc tgcttgcgga gccgctcatc cagtgccggt acaatctggt atccatctgg
     1501 gctgagttga ttgagccctt tgtcagttcc gatccagagc gttccatttt gatcaaacca
     1561 cagcgagaga ataatcttgc cgttgaggcc ctgagattca ccatagccag taacctgacc
     1621 gtcacgaagg cgattgagcc cattttccgt gccgatccag aggctctggt ccgggccttc
     1681 aatcagcgac cggatgttgt tattgagcag tccctgctca acgccaaacc gggtaaacct
     1741 tccattttcc aagcaataga gcccgcctcc catacttccg atccagagac gattctggcg
     1801 atcaacaaag agcttccgaa tatgattggt gagcaattcc ggggtatttc ggcgatcaaa
     1861 caccgtaaac cgcaccccgt caaagcgcgc caggccttca taggtcccaa accagagata
     1921 gccgtcagcc gtctgtgcaa tggcattgac tgaattctga ggcaattcgt cctgccacgt
     1981 tttggtcaca tactgggtga tggctttgtc gggattgatc ccccaaaccg acgcgggaaa
     2041 cagcgcaacc cacaggaggc accatcccca gacggaaata ctttttctgg tggaatggta
     2101 aaagcactgc atacgatgtc gaattcacct ccaggcccca aggtgatgcc tgattgacag
     2161 tattcccgtg aagattgtgc gctgatgggt ttgaaaatgg atgaaaggtt acgtgaactc
     2221 actggtcagg gtagcaggat gtgccccagc cttcaataac cagggcaaca cggtgacaaa
     2281 tgac
//
LOCUS       DAOQDO010000991         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43483_length_2284_cov_2.883356, whole genome shotgun sequence.
ACCESSION   DAOQDO010000991 DAOQDO010000000
VERSION     DAOQDO010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43483_length_2284_cov_2.883356"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..327
                     /locus_tag="PKZ53_15080"
                     /pseudo
     CDS             31..327
                     /locus_tag="PKZ53_15080"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            445..885
                     /locus_tag="PKZ53_15085"
     CDS             445..885
                     /locus_tag="PKZ53_15085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ41813.1"
                     /translation="MRKIVARATLKTTKGNIELEFYLEDAPLTVYTFVNLAEKGYFDG
                     LTFHRVVPNFVVQGGDPRGDGNGGPGFQIRCEINQQPYERGSVGMALSGKDTGGSQFF
                     ICHSPQPHLDGGYTVFGRVIDGIEVVDRITRGDQILRIEVQRPE"
     gene            971..2245
                     /locus_tag="PKZ53_15090"
     CDS             971..2245
                     /locus_tag="PKZ53_15090"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ41814.1"
                     /translation="MSNTYAKHIGYLDLLRRNPKVRRLWMAQVVSELGDWLNFVALVQ
                     IINHFTPNAQAIGWLIVIQMIPMVVVGPFAGIIADRFNRCGVMIVADLVRAAIVLGYL
                     TIDRPEELWRVYVLAALQFSVTAFFEPARAALIPSLAEGDELVASNALTSVTWSVMLA
                     LGGFLGGLITGFINPTATFLIDFISFIVSALLLIRLLSAGAIRHLEHKPESPHPPESG
                     LGPALVFLAHRPQVLAAMLIKTGICITAGGLWLLSVVYGQRVFPVGKEGALSVGLLYG
                     VHGLGSIVGASLSSRMLRTGRPIHAMLWAFVLRTAFFYFWAKAGNLTVVSVAIIAATS
                     CGALLWVLSTTVLQELVPDQIRGRIFSLENSAMTFGMIVSTVLTGRALDIWHLTPQAT
                     TLWTAVAAAVVAVGWGWVTWRWEKWRRAKEYI"
BASE COUNT          484 a          610 c          619 g          571 t
ORIGIN      
        1 cgtgagcttg atccacgcgg agttcctgat ttgcttcgag caattgccaa acacaaacct
       61 gacactctgc cagaactcct gcgtgggcaa ctgaaagcgt cagatgtcat gatccgtgcc
      121 acagcggctg agttgttagg agaaacaccg cagagcgaag agtcattcaa agctctttcc
      181 acggccctca cagcagcagc cagcgagacg atgaatgatg ccaaactggc gattttgacc
      241 gcgttatccc gctataaccg gcctgaaacg gcaaccattc tcaatgcgac acttcgagat
      301 aacgattatc tggtccgacg tcaggcggct gatttgctca aggtgctcga tccacgcccg
      361 gaccgcattg ccagccgtga agctcaggtc gggattgtcc ggcttgaccg cgacatcaag
      421 ttttaccagc aactggcaaa actgatgcgc aagattgtcg cccgcgccac actcaaaacg
      481 acaaagggaa atattgaact tgagttctat ctggaagatg ccccactcac ggtatacaca
      541 tttgtcaatc tggcggagaa aggctatttt gacgggctca ccttccaccg agtcgtgcca
      601 aactttgtgg ttcaaggcgg agacccacgc ggggatggaa acggcggtcc cgggttccag
      661 attcggtgtg agatcaacca gcaaccttat gaacgtggat cagtcggaat ggcactttcg
      721 ggtaaagaca ccggcggaag tcaatttttt atttgccact ctccacaacc tcatctggac
      781 ggtggatata cagttttcgg gcgcgtgata gatggaattg aggtcgttga ccgcatcact
      841 cgcggcgacc aaatcctgag aattgaagtg cagagacctg agtgacaggg ttttggagtg
      901 cggcccactt gacgccgctt tggatgccac ctgtcacctt gtcactgact cgcctggttc
      961 taacaaccaa atgtcaaaca catacgccaa gcatatcggc tatcttgatt tgctacgccg
     1021 caacccaaaa gtccgtcggc tctggatggc ccaggttgtc agcgaattag gcgactggct
     1081 taactttgtc gccctggttc aaatcatcaa tcatttcact ccaaatgcgc aggccatcgg
     1141 ctggctgatc gttatccaga tgattccgat ggttgtggtc ggaccatttg ccggaatcat
     1201 cgcggaccga tttaatcggt gtggcgtcat gattgttgcc gatctggtcc gtgccgcgat
     1261 tgttttaggc tatttgacca ttgaccggcc agaagaactc tggcgagtgt atgtcctggc
     1321 agcactccaa ttttcggtca ccgccttttt tgaaccggcc cgcgctgcct taatcccctc
     1381 actggctgaa ggcgacgaac tcgttgcctc caatgctctg accagcgtca cctggtcagt
     1441 tatgctggcg ctgggtggtt ttctcggtgg cctcatcacc gggttcatca acccaacggc
     1501 cacgtttctt attgatttca tttcttttat tgtttcggct cttttgctga tccggttgct
     1561 cagtgccgga gccattcgcc accttgaaca taaaccggaa tcgccccacc cccctgagag
     1621 tggtcttggg ccggcacttg tatttttagc tcaccgtcct caggtactgg ccgcgatgct
     1681 gattaaaacc ggcatctgca ttactgccgg aggactctgg ctgctgtcgg tcgtgtatgg
     1741 tcagcgagtg tttccagtcg gtaaagaagg ggcgctctcg gttgggctac tctatggtgt
     1801 ccacgggctt ggctcaattg tcggcgccag tctgtccagt cggatgctgc gcactggccg
     1861 tccaattcac gccatgctgt gggcgtttgt gctccgaacg gcttttttct acttctgggc
     1921 gaaggctgga aacctgacag tggtgtcagt ggcaattatt gcggcgacct catgcggtgc
     1981 cttgctgtgg gtgttgagta ctacggtgtt gcaggaattg gtgccagatc aaattcgagg
     2041 acgaatcttt tctcttgaaa attcagccat gaccttcggc atgattgttt ccaccgttct
     2101 gaccggacgc gccctggata tctggcattt gacaccacaa gccaccacgc tgtggacagc
     2161 ggtggcggct gccgttgttg ctgtcggctg gggctgggtc acctggcgat gggaaaaatg
     2221 gagaagagcg aaagaatata tatgagtcag taccacccgt gtttgtgggt gcgagcggat
     2281 gaac
//
LOCUS       DAOQDO010000992         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43484_length_2284_cov_2.856438, whole genome shotgun sequence.
ACCESSION   DAOQDO010000992 DAOQDO010000000
VERSION     DAOQDO010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43484_length_2284_cov_2.856438"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..356
                     /locus_tag="PKZ53_15095"
     CDS             117..356
                     /locus_tag="PKZ53_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41815.1"
                     /translation="MKKPVFLTSLAFFALVLVVYVGSYLIFRSNHIQVWERNQLAYVI
                     FPPDNLVWFYFYRPLTYIDGALTGMRFHIGPHQEA"
     gene            403..789
                     /locus_tag="PKZ53_15100"
     CDS             403..789
                     /locus_tag="PKZ53_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ41816.1"
                     /translation="MTTVRFREAVEQHNLEGMLAALADDAVLHSPITFQPFEGKRAIA
                     GLFSVLLQVFEDFHYTDQLQSPDGTIGLIFRAHIGDREVEGIDILRFNDAGLIQDFTV
                     MIRPKSGMDALLAAVSTKLPPKKKAE"
     gene            989..1885
                     /locus_tag="PKZ53_15105"
     CDS             989..1885
                     /locus_tag="PKZ53_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ41817.1"
                     /translation="MDKDTLINVAAQMARGGELAHLADQQLDDDRLVELWLHGRSPHT
                     QRCYRLDVNRFRAFTNKPLALASLQDLQQFSHSFTTLTPRSLARVLSSVKSLLAFGHK
                     IGYLQYDVGRVLRVPTAKDSLAERILPEADVLQMIALEPNKRNRGLIRLLYAAGLRVS
                     EVCGLKWRDVQPRADAGQITVYGKGNKTRTILLSSTTWRELLTLKGDATPDQPVFLSR
                     FKRQFDTSMVYLIVRAAAKRAGIEGNVSPHWLRHAHASHALDRGAPIHLVQATLGHES
                     IATTGRYLHARPTDSSARFLPV"
     gene            1948..>2284
                     /locus_tag="PKZ53_15110"
     CDS             1948..>2284
                     /locus_tag="PKZ53_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41818.1"
                     /translation="MKKLFAILVLAISIIGVIPQLSQPSSAKPLTSAPIVKASLGSLM
                     TELETNVKWGAVYGNWKKRRDSWLVDCQRANSAQKLGALLAEFESNVTWEMVEAGWRK
                     RRDSWVADCQ"
BASE COUNT          519 a          618 c          577 g          570 t
ORIGIN      
        1 ctgtttttcg ccgctgacct cactttatga cggctttttt cctctcgtca aactgacgag
       61 tcgccccgag tttgtctaaa ctcgagtcat attgatcctg agaggagggt acggaaatga
      121 aaaagccggt ttttctgacc agtttagcat tcttcgcgct cgtcctggtg gtgtatgtcg
      181 gcagctactt gatctttcgt tccaatcaca ttcaggtgtg ggaacgaaat caactggcct
      241 atgtcatttt tccaccggac aacctggttt ggttttattt ctatcgtccc ctgacctata
      301 ttgatggagc gctgaccggg atgcggttcc acattggacc gcatcaggag gcataaaaag
      361 cggcgtcaag ccgccgcact ccataccaat tgaggttttt ccatgacaac cgttcgtttt
      421 cgtgaagcag tcgaacaaca caacctggag ggcatgctgg cggcgctggc tgatgatgcc
      481 gtcctgcata gtcctatcac gtttcaacca tttgaaggca aacgcgccat tgccgggttg
      541 ttttcagtgt tgctccaggt ctttgaagat tttcactaca ctgatcaact ccagtccccc
      601 gatggcacca tcgggttgat tttccgggct catattggcg accgggaggt tgaaggcatt
      661 gacatcctcc gattcaatga tgctggcctg attcaagatt ttaccgtgat gatccgccca
      721 aaatctggca tggatgcgtt actggcagcg gtcagtacca aactgccacc aaaaaagaag
      781 gctgaatagt tttctttccg ctgaatttat cttgaattcc aaagctcacg tagcgcacaa
      841 tggccgccac ttcattacct ggtgattggg atttcaggct acgggctgaa aaaaaacgag
      901 ttcaataccc tcaacccaac ttcttcagcc cgatgtcttc agctccaagc cctggttttt
      961 cagccctcaa atagaaaggt gtgcacttgt ggataaagat acgttgatca atgtggcagc
     1021 tcaaatggct cgcggcggag aactcgcgca tctggcagac cagcaacttg atgatgaccg
     1081 gctggttgaa ttgtggctgc atggacgcag ccctcatacc caacgctgct accggctgga
     1141 cgtcaatcgg tttcgagcct ttaccaataa gccgctggcg ctggcttccc tccaggactt
     1201 acaacagttc tctcattcgt tcaccacgct gacaccgagg tcgctggcgc gggtcctgtc
     1261 aagcgtcaaa tctctgctcg cctttgggca taaaatcggc tatctccaat atgatgttgg
     1321 tcgagtactg cgggttccaa ctgccaaaga ttcgctggcc gaacggattt tgcccgaagc
     1381 cgatgtcctg caaatgattg cgcttgaacc aaataaacgg aaccgtgggt taattcggtt
     1441 gctctatgcg gcagggctcc gcgtcagtga agtctgtggc ctcaaatggc gtgatgtgca
     1501 acctcgggcc gatgccgggc aaatcacggt gtatgggaag gggaacaaaa cccggaccat
     1561 tctgctgtct tcgacaacat ggcgcgaatt acttacgctc aaaggcgatg caacccctga
     1621 ccagccggtg tttctgtcgc gctttaaacg tcagtttgat acctcaatgg tttacctgat
     1681 cgtccgggcc gccgccaaac gggctggaat cgaagggaat gtttcgccac actggctccg
     1741 gcatgcccac gcgtcacacg ccttagatcg cggcgcacca atccatctgg tgcaggcaac
     1801 ccttggccac gaaagcatcg ccacaacggg ccggtatctg cacgcacgtc cgacagactc
     1861 atccgcccgg ttcctgccgg tttagcccag tcacctgtca ccaattgacc tcaatctatt
     1921 tcatctgtgt ctactgagga gagtccaatg aaaaaactat ttgccattct ggttttagca
     1981 atttcgatca tcggtgtgat accccagctt tcccagccat caagtgcgaa accactgacc
     2041 tcggcaccaa tcgtcaaggc cagtttggga tctttgatga ctgagcttga aacgaacgtc
     2101 aaatggggcg ccgtctacgg caactggaaa aaacgtcgcg attcgtggct ggttgactgc
     2161 cagcgagcaa attcggcgca aaagttaggt gctctgctcg ctgaatttga gtcaaacgtt
     2221 acctgggaaa tggtggaagc cggctggcga aaacgtcgtg attcgtgggt ggctgactgc
     2281 cagc
//
LOCUS       DAOQDO010000993         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43525_length_2283_cov_2.349641, whole genome shotgun sequence.
ACCESSION   DAOQDO010000993 DAOQDO010000000
VERSION     DAOQDO010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43525_length_2283_cov_2.349641"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            507..2156
                     /locus_tag="PKZ53_15115"
     CDS             507..2156
                     /locus_tag="PKZ53_15115"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ41819.1"
                     /translation="MENRRIVALYQNSYALVIGVSEYTGGWTDLPGVKTDLTEVKKAL
                     ETEGFQVELVLNPTYDQFQEKMNQFIKKYGYREENRLLVYYAGHGHTGKSADGRQMGY
                     IVPADAPLPEKDNRGFRLRSVSMDNLVAYARQMETRHALFVFDSCFSGTLFTTTQAEN
                     NTQSISTLMTQPVRQFLTAGTDQQTVPDKSLFREWFVEGLQGAADGNQDGYVTASELS
                     RYVQANVSNNSRQKQTPQYGKIQDDKLNQGEMVFLTGKFKEMKYWDTMWNSSNPTDYR
                     TYLTKYLNGEYAEVARNRITRLERKPFQAQPAKQFTNSLGIKFVLVPAGTFTMGSNLG
                     ELDEKPHEVTISQPFYLGKFEVTQAQWQAVMGANPSYFKGDNLPVEQVSWDQVQHFIQ
                     RLNANGDGEYRLPTEAEWEYACRAGTREEYAGKLEKMGWYAANSGNRRLSDDGNWKLP
                     ELMANRCQTHPVGKTKPNKWDVYDMHGNVLEWCQDRYEKDYYSKSPRRDPRGPLMGLY
                     RVYRGGSWSLQGSFCRSARRSYNAPAQPLRNVGFRLVRMVW"
BASE COUNT          604 a          512 c          656 g          511 t
ORIGIN      
        1 ggaaaggcgg tttatacgtt ccgtctggtc ccagtgcaat ccaacccgat cattgaagaa
       61 aaaaacgccg gggtacggtt tgtagcgcag gttttggata cccaaggccg acccctggtg
      121 ggtgcaatcg tcagggtttc agcaccaaac accttccaac atcagaatgg gaggtggctt
      181 cttcagggga aaacaaactc cagaggtgtt tttgactcga ccgggaattt atcccaaggc
      241 cgacagttgg ttcagccggg gcgttatctg attattgttt cctgtactgg gttccaggct
      301 tttcaggata tggtgagttt ggtggaagtt cggaagggta tcgctgtgta cacgtttcat
      361 ttagtccctg aaaggtgatt caggaggagg caatgcagaa aaaatggatg atcaaaacaa
      421 taggtgcttt gcttccactc ctgggaatct gtttggtcgg ggcaaatgtt cggcaggagg
      481 aaagaggcca aggtgtcaat ttcaagatgg aaaaccgacg aattgtggca ctctaccaaa
      541 acagttacgc cttggtcatt ggagtcagtg agtacactgg gggttggacc gacctgcctg
      601 gtgtcaaaac cgacctgact gaggtaaaaa aagcattgga aacggaagga ttccaggtgg
      661 aattggtgtt gaacccgacg tatgaccaat tccaggaaaa aatgaatcag ttcataaaga
      721 aatatggtta ccgggaggaa aaccggctgt tggtctatta tgcgggtcat gggcacacag
      781 gaaaatccgc cgatggacgg cagatgggtt acatcgtacc ggcggacgcg ccattgccgg
      841 aaaaagacaa tcgagggttt cggttacgct cagtcagcat ggacaacctg gtggcatacg
      901 ctcggcaaat ggagacccgg cacgctttgt ttgtctttga cagttgtttc tccgggacgt
      961 tattcacgac cactcaggct gaaaacaaca cgcaatcaat ttccaccttg atgactcagc
     1021 ctgtgcggca gtttctgacg gcggggactg accaacagac agtgccggat aaaagcctgt
     1081 ttcgggaatg gttcgtggaa gggttacagg gggcagcgga cggcaatcag gatgggtatg
     1141 tgaccgcctc tgaattgtcc cgatatgtcc aggcgaatgt gagcaacaat agccggcaga
     1201 agcaaacacc tcaatacggg aaaatccagg atgataaact caaccaggga gaaatggtgt
     1261 ttctgacggg gaaattcaag gagatgaaat actgggatac tatgtggaac agcagtaacc
     1321 ccaccgatta tcgaacctac ctgacaaaat atctgaatgg agagtatgcg gaggtggccc
     1381 gaaaccggat cacccgtttg gaaagaaaac catttcaggc tcaaccggcc aaacagttca
     1441 caaacagtct cggaatcaaa tttgttttgg tcccggcagg gacgtttacc atgggatcca
     1501 atctgggcga acttgatgaa aaaccacacg aggtgaccat cagccaacct ttttacctgg
     1561 ggaaattcga ggtgacccag gcccagtggc aggccgtcat gggagccaat ccgtcgtatt
     1621 tcaaagggga caatctgccg gttgaacagg tgtcttggga tcaagtgcag catttcattc
     1681 agcggctgaa tgccaatggg gatggtgagt atcggttgcc gaccgaagca gagtgggaat
     1741 atgcctgtcg agccggaacc agggaagagt atgccggaaa attggaaaaa atgggttggt
     1801 atgccgcgaa ctcaggaaac cgtcgtttga gcgatgatgg gaattggaaa ttacctgaat
     1861 tgatggccaa ccgctgtcag actcatccgg tggggaaaac caaaccaaac aagtgggatg
     1921 tatacgatat gcatggaaat gtgctggagt ggtgccagga tcgctacgaa aaggactatt
     1981 acagcaagag tccgcgccgc gatccacgag gtccgttgat gggattgtac cgggtgtatc
     2041 gcggcggcag ttggagcctg caaggttcat tctgtcggtc ggcacggcgc agctacaatg
     2101 ctcctgccca acctctccgc aatgtcgggt ttcggcttgt ccggatggtg tggtaggctc
     2161 cgcacggttc cgtacaaaat tgcctggttg cacccggtgt cgactttcct ctcaggaagt
     2221 ctcacggact gatcaaaata caaccggtta attggacatt ttccgttttg ccccacaccg
     2281 ttc
//
LOCUS       DAOQDO010000994         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43573_length_2281_cov_2.876460, whole genome shotgun sequence.
ACCESSION   DAOQDO010000994 DAOQDO010000000
VERSION     DAOQDO010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43573_length_2281_cov_2.876460"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..972
                     /locus_tag="PKZ53_15120"
     CDS             7..972
                     /locus_tag="PKZ53_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNJ41820.1"
                     /translation="MTTDHGLLTGTISGVEMQTLQIRFENALTSLDGLSVGDAFGQGF
                     FINSQLLEQLITERALPSPPWFFTDDTLMTLSMVSNLRQFGTINQDVLARSFAERYDY
                     ERAYGPSMHRALGRIRDGEDWRDVAASLFAGQGSFGNGAAMRVAPLGAFFADDFPELI
                     EQARLSAEVTHAHPEAIAGAIAVAVAAGVAVRARKHAVKPASDEFFAEILSCTPSSEV
                     KSKLARAQTMPGSTDIAHVAAMLGNGTGVSAQDTVPFAVWCAAQWLDDFETAMWKTVS
                     ALGDRDTTCAIVGGIVASFTGRDGIPSNWLADREPLPDWFRSAAA"
     gene            complement(1581..2009)
                     /locus_tag="PKZ53_15125"
     CDS             complement(1581..2009)
                     /locus_tag="PKZ53_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41821.1"
                     /translation="MPHSFRTILKTGLLYFLLVFGVGFILGPIRILWLVPRLGERYAE
                     LIEMPLMLAAIILVARWMIRRFQIPTDTGVRLGMGFLAVVLLISVEFTMVLKLRGLTL
                     EQYFQSRDPISGTVYYLMLVVFALMPWILRRWEHRSAAQG"
     gene            complement(2074..>2281)
                     /locus_tag="PKZ53_15130"
     CDS             complement(2074..>2281)
                     /locus_tag="PKZ53_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41822.1"
                     /translation="ILLEGLILVLNGWQCPFTKLAARFTDDRSDNFDIYLPVWLARHN
                     KLIFTILFLGGTAFTFSRWIGWLK"
BASE COUNT          565 a          585 c          589 g          542 t
ORIGIN      
        1 tggccactga ccactgacca cggactactg actggtacta tttcaggtgt tgaaatgcaa
       61 acgcttcaaa ttcgctttga aaatgctttg acttcacttg acggcttatc cgtcggtgat
      121 gcgttcgggc aaggtttttt tatcaactcc cagttgctgg aacaattgat taccgaacgg
      181 gcgctgccgt caccgccatg gttttttacc gacgacacgt tgatgacgct ttcaatggtg
      241 tcgaatctca ggcaatttgg aacgatcaac caggacgttc tggcccggag ttttgccgag
      301 cggtatgact atgaacgggc gtacggtcca tccatgcacc gggcgctggg gcgcatccgt
      361 gacggagaag actggcgtga tgttgctgct agcctgtttg ccgggcaggg atccttcgga
      421 aacggtgccg ccatgcgcgt ggcaccgctt ggggccttct ttgcggatga ttttcctgaa
      481 cttattgagc aggcacggct ttccgccgaa gtcacccacg cccatccgga agcaattgcc
      541 ggagcgattg ccgtggctgt ggcggctggc gtggcggttc gtgcccggaa gcacgctgtg
      601 aaaccagcct cagacgaatt tttcgcggaa attctttctt gcacaccttc gagcgaagtc
      661 aaaagcaaac tcgcccgagc acaaaccatg cccggctcaa ctgatatcgc gcacgttgcc
      721 gcaatgttgg gcaacggcac cggggtttcg gctcaggata ccgttccgtt cgcggtttgg
      781 tgcgcggcac agtggctgga tgattttgaa accgcaatgt ggaaaacggt cagtgcgctg
      841 ggtgaccgtg ataccacttg tgcgattgta ggaggaatcg tggcgtcctt taccggacgg
      901 gatggcattc catcgaactg gttggcagat cgtgaaccac ttccggattg gtttcggagt
      961 gcggcggctt gacgccgctt ttacagttgc cattccagac tgtatttcca cccaaaagta
     1021 tctttcagcg aaaaacgatc aagactttgt tttcctataa tttggtaaca ggaagagtgg
     1081 cgattacatt cctggtcact cgattttctt aaatagtaaa tgaatctcgg gactttcctc
     1141 aggtcagata ctggggaata tacgatttcc ttgatctcgt gaatcgttgt aatgatgcca
     1201 tgaaattgat ccagcccatg gtaataaatc ccttttcctt gcaggaactt attaccagac
     1261 agtacccctt caacaatgaa tctctctccg caactgtttc ctcgaatcca cattgatact
     1321 tttccggtaa ccatggaatc aaactcatac agacagttga gttgagagag aggctgaacc
     1381 cgccgcttga gctgagaaaa cgagctgtga aatctggaat cgaaagcggt cactgccaga
     1441 tcggtagttc gtagattttt ttcagaggac tggtctcttg atggggtaca agccagcatc
     1501 cagccaaagt acccacaggc gaaaaaaacc agaagtggca ccaagacagg ccgttgcata
     1561 tgatttccct ctttctttgc tcaaccttga gctgcagacc tgtgttccca ccggcggagg
     1621 atccacggca taagcgcaaa caccaccagc atcagatagt acaccgtgcc tgaaatcggg
     1681 tcacggcttt ggaagtattg ctccagcgtc agccctcgga gcttgagcac catcgtgaat
     1741 tcgactgaaa tcaacaaaac taccgccaga aaacccatgc ccaatcgaac acccgtgtct
     1801 gtcggaatct gaaaccgacg gatcatccat cgcgcaacga gaattatcgc cgccagcatc
     1861 aagggcattt cgatcagctc agcgtagcgt tcgcccagtc tcggcaccag ccagagaatc
     1921 cgaatcggcc ccaggataaa cccgacgcca aacaccagca gaaagtacaa aagaccggtt
     1981 ttgaggattg tgcggaatga gtgtggcatg gcggtttgaa ttgagggtgt gggttggatt
     2041 tccagaaaat attgaacttt agttgccagg gtttcacttc agccagccaa tccaccggga
     2101 aaatgtgaac gcggtaccac ccaggaaaag aatcgtgaaa ataagtttat tatgccgcgc
     2161 cagccagacc ggaaggtaaa tatcaaagtt gtcactacga tcatctgtaa accgggccgc
     2221 gagtttggtg aatgggcatt gccagccatt caagaccaga atcaatcctt ccaggagaat
     2281 c
//
LOCUS       DAOQDO010000995         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43673_length_2279_cov_2.383543, whole genome shotgun sequence.
ACCESSION   DAOQDO010000995 DAOQDO010000000
VERSION     DAOQDO010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43673_length_2279_cov_2.383543"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="PKZ53_15135"
     CDS             complement(<1..1600)
                     /locus_tag="PKZ53_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNJ41823.1"
                     /translation="MMTQGFRRCWQPGGIFFQVFLLLVVVGLTSLTAQAQGILIPQEP
                     NRPRPPVPRPVPIPRTLKIKSIRITTTIKNQVARTHIEQIFLNETPYTLEGTYCFPIP
                     ESASLSEFAIWEGNRRLVGEVVERAKARQIYDSIVRTMRDPGLLEYSGKDLFQASVFP
                     IPGNSEKKIELAYSQVLTGENGIVRYRYPLGSGQRAQGEPPQSVVGQVEIDSPTAIKT
                     IYSPSHSIDTKRDGEKKARLSFEVSGQKGLDDFQLYYGLSEKDFGVSLVTHREPGKDG
                     YFLLLIAPKVAISERDRLAKDIVFIFDTSGSMSGEKIEKAKNALKFGLQSLSPVDRFN
                     VIRFSGEEHLFENGLVEASPDTIKRAVQYVDDFRAEGGTNINDSLLAGLKLFSESAAR
                     PRMVVFVTDGLPTVGTTDAATIIKNVTAANQTQARIFTFGVGYDVNTQLLDKIAEQNR
                     AVSDYIEPNEDLEVKVSNFFGRINYPVLSDVKLDFGGVQVDYVYPKALTDLFRGGQFT
                     LVGRYRNNDDQRVTLRLSGMVQNKE"
BASE COUNT          536 a          574 c          587 g          582 t
ORIGIN      
        1 gttctttgtt ttgaaccatt cctgaaagcc ggagcgtgac gcgttgatca tcgttgtttc
       61 ggtagcgccc aaccagcgtg aattgcccac ctcggaaaag atccgtcagc gcctttggat
      121 agacatagtc aacctgcacg ccgccaaaat cgagtttcac atccgacaac accgggtagt
      181 tgatgcggcc aaagaaattg gacactttga cttcgaggtc ttcattgggt tcgatgtagt
      241 cactcaccgc ccgattctgc tcggcaattt tgtcaagcaa ctgagtattg acgtcatagc
      301 cgacgccaaa ggtaaaaatt cgagcctggg tttggttcgc ggcggtgaca tttttgatga
      361 ttgttgccgc gtcggttgtt ccaaccgttg gaagaccgtc agtcacaaac accaccatcc
      421 gtggacgggc ggcactctcg gaaaacaatt tcaacccagc cagcagagaa tcgttgatgt
      481 tggtaccgcc ttcagcccgg aaatcgtcca catactggac ggcgcgtttg atggtgtcgg
      541 gcgatgcttc aaccaggcca ttttcaaaca gatgttcttc acctgaaaag cggatcacat
      601 tgaaccgatc cactggtgaa agtgactgta agccaaactt gagagcattt ttggctttct
      661 cgattttttc accactcatc gaacctgacg tgtcaaagat aaacacgata tcttttgcca
      721 gtcggtcacg ttcggaaatg gccactttgg gggcgatgag gagcaggaag tagccatctt
      781 tgcccggttc gcgatgggtg acgaggctca cgccaaagtc tttttcactc aggccataat
      841 agagctgaaa atcatcaagt cctttttgcc cggagacttc aaaactcagg cgggctttct
      901 tttcgccatc gcgtttagtg tcaatcgaat gacttgggga gtaaatcgtc ttgatcgcgg
      961 ttggggaatc aatttccacc tgacccacga cactttgggg tggttcgccc tgggcgcgct
     1021 ggccgcttcc gagtgggtag cgataccgga caatgccgtt ttcacctgtc agcacctggg
     1081 agtacgccag ttcgattttc ttctcactat ttccgggaat cggaaacacg ctcgcctgga
     1141 agaggtcttt tcctgagtat tcaagcaacc caggatcccg catggtgcgc acgatgctat
     1201 cgtaaatctg gcgagctttg gcgcgttcga cgacttcgcc caccagtcgc cgattgcctt
     1261 cccagatggc aaattcagac agggaagcgc tttccgggat cgggaagcaa taggtgcctt
     1321 ccagcgtgta tggcgtttcg ttgagaaaga tttgttcgat gtgcgtccgg gcgacctggt
     1381 ttttgatggt ggtcgtgatg cggatgctct tgatttttaa ggttcgtgga attggaactg
     1441 ggcgtgggac cggtgggcgc gggcggttcg gttcttgagg aatcaaaatt ccctgggcct
     1501 gagcggtgag ggaagtgagt ccaaccacaa ccagaagcaa aaacacctgg aagaaaatcc
     1561 ctccaggttg ccagcagcga cgaaacccct gagtcatcat aaaaatcgtt ctccggtacg
     1621 aaaaaatgga attctatttg attgagaatg gttgaatctg tggtcgcctg aacccgcatg
     1681 aaggttccgg tcaaattgct gaaaaaccct caattaccgg gtatttttcc tttcagaaac
     1741 cttgaacctt gagccctgaa ccctgggatg actggcttgt aactttccta gtctgaactg
     1801 tataaactct ttggcataaa ctaaaatatg aactcttttt cattgacctg aagaattgct
     1861 ctctgctagc attgcgcatc atcatcaacg gtttgattca caagccgtca cgcttttcag
     1921 accagaaatc acatcgtgtt catcaaattt gaccaggggg gatcactttt tgttactcgc
     1981 gtaaacggag caaaagttta cctatgattt gccctcagtg cggatacctg caatcagacg
     2041 accggattcc ctgccgtcgg tgtagacaag cggtccattc tacgtcacag cgttcatcag
     2101 ttggaagtgt tttagatttt cccggcaata ctcggggagg agtatccaaa atgtcatccc
     2161 agcgccctcc gatggaggtt cacagtgctc ccaatgttcc tgcgtggcga gccgaattaa
     2221 gcgcccgcgt gcgacagatc aaggcccggc gctcaatgga agccgagctt gaagccgcg
//
LOCUS       DAOQDO010000996         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43700_length_2278_cov_2.902384, whole genome shotgun sequence.
ACCESSION   DAOQDO010000996 DAOQDO010000000
VERSION     DAOQDO010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43700_length_2278_cov_2.902384"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="speB"
                     /locus_tag="PKZ53_15140"
     CDS             complement(<1..799)
                     /gene="speB"
                     /locus_tag="PKZ53_15140"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNJ41824.1"
                     /translation="MHTQSPIALLGFCSDENSSFRRGAAQAPPLIRDALLTDASNMWT
                     ETGVNLGELQEKNLLIDVGDIAPTPGDDFFDQIQTTIHQLLAQQISPISLGGDHAITF
                     PILQGFARHFPEITILHIDAHPDLYDELGGNRLSHACPFARIMEAGLATRLIQVGIRT
                     LNQHQREQAARFGVEIHEMKDWHPGLKLDFDTPVYVSIDLDGLDPAFAPGVSHREPGG
                     LSTREVINLIHSIPTRIIGADVVEYNPLCDFQGMTATVAAKLVKELVG"
     gene            complement(893..1483)
                     /locus_tag="PKZ53_15145"
     CDS             complement(893..1483)
                     /locus_tag="PKZ53_15145"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNJ41825.1"
                     /translation="MDTKWKPYFLLTIATVLVIGSVLVIYKSLSGVPTLPPITVHNTT
                     ITNSMVVEKIQKVAKVVSSEVTVRDVVTYENTWYGSTKRTLVVVTGKIMGGINLDAGY
                     DVRIDEKAKRISITLPKATILAVEIGEMKTYDEQGGLWNPITAADRDAIFKQARTQFE
                     QSGQTLRITEKAQTGAKELFEAMFSNQGYGVEVSFR"
     gene            complement(1681..>2278)
                     /locus_tag="PKZ53_15150"
     CDS             complement(1681..>2278)
                     /locus_tag="PKZ53_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41826.1"
                     /translation="AQLYLPFAQIPEQFFPQLAGNLNVLVRTEGDPSLLIDAIRTRVN
                     ELDRDIVMFNTNKMETIVSQSLGEKQVLTILLGIFALVAVTLAAIGVYGVLSFWVSLR
                     VPEIGLRLALGASPIDVVRLVARFGGGLVLSGIFTGIMAAWGLSRLISNMLFEVSSSD
                     PMTYAVVSLGFLAIAGIACLVPAIRAARVDPLVALRSE"
BASE COUNT          573 a          576 c          572 g          557 t
ORIGIN      
        1 ttcccaccag ttccttgacc agtttcgcgg cgaccgttgc ggtcattcct tgaaagtcgc
       61 acagcgggtt gtattcaacc acatcggcgc cgatgatccg ggttgggatt gagtgaatca
      121 ggttgatgac ctcgcgggtt gaaagacctc cgggttcgcg atgcgaaacg cctggggcaa
      181 aggccggatc gaggccatcc agatcaattg aaacatagac cggcgtgtca aaatccagct
      241 tcaggcccgg atgccagtct ttcatttcgt ggatttcaac cccaaagcgg gctgcctgtt
      301 cgcgctgatg ctgattgagc gtccggatac caacctgaat cagtcgtgtg gcgaggccgg
      361 cttccataat tcgagcaaat gggcaggcat gggaaaggcg gtttccaccg agttcgtcat
      421 agagatctgg atgggcgtca atgtgcagga tagtaatttc gggaaaatgc cgggcaaatc
      481 cctgaagaat tggaaacgtg atcgcgtgat cgcctccgag ggaaatcggg gaaatttgtt
      541 gcgccaaaag ctggtggatg gtggtttgga tctgatcgaa aaaatcatct ccgggcgtgg
      601 gcgcgatgtc accgacatca atcaggaggt ttttttcctg aagttcgccc agattgactc
      661 cagtttcagt ccacatattt gaagcgtcag tcaacagggc atcacggatc aacggcggtg
      721 cctgggcggc accgcgccgg aacgacgaat tttcatctga gcaaaaacca aggagggcaa
      781 tcggagattg ggtgtgcatt gtttttgagt ctgataagct tacttatgtt aagttatacc
      841 accttgggct gaaagtatca ggctacgagc tacactggta tccaacctga aattatcgaa
      901 atgaaacctc cacaccatat ccttgattcg aaaacattgc ttcgaataac tctttggcgc
      961 ccgtttgagc cttttcggtg attcttaatg tctgcccgga ttgctcaaac tgggttcgag
     1021 cctgtttaaa aattgcatcc cgatctgcgg cggtgattgg gttccataac ccgccttgtt
     1081 cgtcataggt tttcatttct ccgatttcaa ctgccaggat ggtggctttc ggaagcgtga
     1141 tcgagatccg ttttgccttt tcatcaatcc gcacgtcata cccagcatct aaattgatcc
     1201 cgcccatgat tttcccggtc acgaccacca gcgttcgttt ggtcgagccg taccaggtgt
     1261 tttcataggt cacgacgtca cgcacagtga cttcactgga aaccaccttg gccacctttt
     1321 gaattttttc aaccaccatt gagttggtaa tggttgtgtt gtgtaccgtg attggtggaa
     1381 gcgttgggac accactcaaa gatttataga tgaccagaac tgaaccaatc accaaaactg
     1441 tggcgatagt taataaaaaa tagggtttcc attttgtgtc catcggtttc agcctcgcgt
     1501 ttcttttgga gtttgatctg atggaaggtg cctggcaaca caaaatggtt ggtggaaccg
     1561 gtatccccaa tacgtttttg cctttttgga atgaaaaccc cgtattacag gaaggctaaa
     1621 ctattgaaag aattgtattt tcctcagccc ttagcccgat gccctcagcc ctaaatcgtc
     1681 ttattcactc ctcaaggcga ccagcgggtc aaccctggcg gctcgaattg ccggtaccaa
     1741 gcaggcaatc ccggcaattg ccaggaaccc aagggacact accgcatacg tcattggatc
     1801 tgatgagctt acctcaaaca gcatatttga aatcaaccgt gaaagccccc aggcagccat
     1861 gatgccggtg aaaattccgg aaagcaccag accgccacca aatcgggcca ccagccggac
     1921 aacgtcaatc ggagaggcgc ccaacgccag ccgaaggcca atttcaggca cacggagcga
     1981 gacccagaag gacagcacgc catacactcc gattgcggcc agcgtgactg ccaccagggc
     2041 aaaaattcca agcaaaatcg tcaggacctg cttttcaccc aatgactgag agacaatcgt
     2101 ctccatttta ttggtgttaa acatcacaat atcccgatcc agctcattga ctctggtgcg
     2161 aatggcatca atgagtaagg atggatcacc ttcggttcga accagaacat tcaaatttcc
     2221 ggcaagttgc ggaaagaact gttctggaat ttgagcaaat ggcaggtaca actgggcc
//
LOCUS       DAOQDO010000997         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43782_length_2276_cov_3.095002, whole genome shotgun sequence.
ACCESSION   DAOQDO010000997 DAOQDO010000000
VERSION     DAOQDO010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43782_length_2276_cov_3.095002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PKZ53_15155"
     CDS             <1..1380
                     /locus_tag="PKZ53_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41827.1"
                     /translation="SQSSIQPVPLWIQHSEWTLSSRIFQILGTCSLVVAIVVSNLLFI
                     PPIGSIQRVQFVPPTAIPVEENAWTEYRIAFQKLGVTGNQRVSFPNELEKYGCWELSS
                     TPQIEFLKLNQEALYHLTKGTRRSKAQYLPAPETFRENEPDYLLSRNLVILAIAESRR
                     LYEAGESQKAIELSLAAYRFAMDTYEPDFSILAYFVSAVCQNIVMRHWFGLISQYHLK
                     PEFCQLIANTIEANQKRVATPEQLLNQQTLIATSENQQRFIDNPGVFSNQDPKYKWSW
                     LIVYLPGLRKTVFLRALELQDRFNQKYLKSMKSWDFAETQKTRIEWRTLRSKPHWSLG
                     EDSSLSLANQSIGSSPRILTSLYSTQTVAQAIRTLAILECYYANHNGYPETLQIAFEE
                     FGKSVPVNLTNGQPVGYRLEQGQPVIWFTGTDGKDNGGTTPCTYEILEDLTPGVDVVM
                     KRGGEFLLK"
     gene            complement(1526..>2276)
                     /locus_tag="PKZ53_15160"
     CDS             complement(1526..>2276)
                     /locus_tag="PKZ53_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41828.1"
                     /translation="LARVVSATELRVTVPPIPYGGEFPLIIHTRGGEAIATLKVIGDP
                     PPATVDLSSFPASRTINAGQSAFYTIGLRTSDDVGPVTLTVDGLPAGADAQIAPNPTS
                     MNESLMRVTTSSRIVPGTYVLTITGKGANAGSGSTTVALNIETVTTQSVTLTANPTSQ
                     TVVRGVAAVYRIAINRINFPDSVELKAALVNGTVTGLNLQLAPQQTTLDSAILTAVAV
                     PGVPSGTYTIRITGTASGITIQPIDVTLVVN"
BASE COUNT          623 a          545 c          541 g          567 t
ORIGIN      
        1 tctcagtcct ccattcaacc agttccattg tggatacagc actcagaatg gacgctttcc
       61 agtcggattt tccagatttt gggtacctgc tcgctggttg tggcgattgt ggtctcaaac
      121 cttttattta ttccaccaat tggctcaatt caacgagtcc aattcgttcc cccaacagcg
      181 attccggttg aagaaaatgc ctggacagag tatcggattg cgtttcagaa acttggggtg
      241 actgggaatc aacgagtttc tttcccaaac gaattggaga aatacgggtg ttgggagtta
      301 tccagtactc cgcagatcga gttcttaaag ctcaatcagg aagcgctcta ccatttaaca
      361 aaggggacac gtcgttcaaa agctcaatac ttaccagccc ctgaaacatt tcgggaaaat
      421 gaaccagact acttattaag ccgcaacctg gtcattctgg cgatagctga atctcgacga
      481 ctgtacgaag ctggcgaatc tcaaaaggca attgaattaa gtttagccgc ctatcggttt
      541 gcaatggata cctatgagcc agacttttca atactagctt attttgtctc ggcggtttgt
      601 caaaatatcg tgatgcggca ttggtttggg ctcatcagtc agtatcatct gaaacctgaa
      661 ttctgccagt tgattgcaaa tacaatcgaa gccaatcaaa aacgggttgc caccccagag
      721 caactcttga atcagcaaac attgattgct accagcgaaa atcaacaacg atttattgat
      781 aatccaggag ttttctccaa tcaagatcca aaatacaaat ggtcctggct tattgtgtat
      841 ctacctggac ttcggaagac agtgtttctc cgggctctgg aactgcaaga tagattcaat
      901 caaaaatacc tgaaaagcat gaaatcatgg gattttgcgg aaacccaaaa aactcgtatc
      961 gaatggagaa cactgcggtc aaaaccccat tggtcactgg gagaagactc cagtctatcg
     1021 ttggcaaatc agagtatagg cagctctccg cgtattctga ccagcttgta tagtacccaa
     1081 acggttgctc aggcaattcg aaccctggcc atccttgaat gttattacgc caaccacaac
     1141 gggtatccag aaacactgca gatcgctttt gaagagtttg ggaaatccgt tccggtcaat
     1201 cttacaaatg gacaacccgt tggatatcgc ctggaacaag gccagccagt gatctggttt
     1261 accgggactg atggaaaaga taatggaggt acaacgccct gcacctatga aatccttgaa
     1321 gatctaactc caggtgtgga tgtggtaatg aaacgcggcg gagaattttt gctgaaatag
     1381 ccagcgagaa gtgagcagca aagtagcaag tagcgagtag cccatccaaa aactttcaga
     1441 gctagttaca gttccccaaa atcagaattt gaggtgttca accactatct caaacaaggt
     1501 tttgagctgg ctactcgctt ttgcttcaat tcacaaccag agtcacgtca atcggctgga
     1561 tggttattcc agatgccgtt ccagtaattc gaatcgtgta cgttccagat ggaacaccgg
     1621 gcacggccac agcagttaag atcgcagaat ctaaagtggt ctgttgtggc gccaattgca
     1681 aattcaaccc cgtcacagtt ccattcacca gggccgcttt taactcaact gaatctggga
     1741 aattgatccg attgatcgca atccgataca cggcggcgac acctctgacc acagtctgcg
     1801 aggttggatt ggcggtcagg gttactgatt gggtggtgac ggtttcaata ttgagtgcca
     1861 ccgtggtcga accacttccg gcgttggccc cctttccggt aatcgtcaga acgtatgttc
     1921 ctggcacaat tcgacttgaa gtggtaaccc gcatcaggct ttcattcatt gaagttggat
     1981 tcggagcaat ttgggcatcc gcgccagcag gtaacccgtc aacggtaagg gttacagggc
     2041 caacgtcgtc gctggttctc agaccgatgg tgtaaaaggc agattgaccc gcattgattg
     2101 ttcggctggc aggaaacgac gaaagatcaa cggtcgcggg tggcggatca ccgatcacct
     2161 tcagggtagc aatcgcttca ccaccccgag tatgaataat gagggggaat tcaccgccat
     2221 agggaatcgg aggcaccgta acgcgcaatt cggttgcact taccaccctg gccagt
//
LOCUS       DAOQDO010000998         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43790_length_2276_cov_2.675371, whole genome shotgun sequence.
ACCESSION   DAOQDO010000998 DAOQDO010000000
VERSION     DAOQDO010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43790_length_2276_cov_2.675371"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2276
                     /locus_tag="PKZ53_15165"
     CDS             <1..>2276
                     /locus_tag="PKZ53_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ41829.1"
                     /translation="LAGWTPDGKSLLIASDRSSINGITNQLYTIPIDVTIATLIPLPM
                     AAAGSYSPDGSQLAYVPVTPAFRAWKRYRGGRASYIWIANLADSSVVKLPRENSNDFN
                     PMWIGDKIYFLSDRNGPVTLFSYDLKTKKVTQEIQNSGLDFKAASAGPDVIVYEQFGS
                     LHYFDLQTGKTRPMNVTIAGDFLAVRPKFEKVANSILNLGISPTGARAVVEARGEILT
                     IPGEKGDVRNLTNTTGVAERDPSWSPDGKWIAYFSDESGEYALHLRTQTGMGEVKKIN
                     LGTPSSFFYTPVWSPDSKKIAFCDKRLNFWYVEVEKGTPVKIDNFERGLVTNYKWSPD
                     SQWLTYAKPLKSVYNAVFVYSLADGKLTQITDGMSNAASPAFDRNGKYLYFTASTNSG
                     PQMFGFDMSSYPHQPTSSVYVCVLKKDEASPLAPESDEEKPEGAAKDGEKKPGEADKG
                     TGKPDEKKEPPKVTIDVEKINQRILALPIPARNFIDLFTGKDNLVYLAEFSTDVQSPG
                     ITLHKFDMQKRKLDKVLDGVTGWTLSANGEKLLYGQGPAWFIAAIDQPIKPGEGKLKT
                     EDLTVYVDPRAEWNQMYRETWRIERDFFYDPNYHGLDIKATEKKYQPYVNGLVHRADL
                     NYLFQEMLGELTVGHLYVSGGDTPDPKRVPGGLLGADYTIENGRYRFARVFDGENWNP
                     QLKAPLTQPGVNVVAGEYLLAVNGRELTAKDNVYGFFESTAGKQVVIKVGPNPDGTGT
                     REVTVVPVDSEDGLRNLA"
BASE COUNT          603 a          572 c          577 g          524 t
ORIGIN      
        1 tctggccgga tggacaccgg acgggaaaag tttgctgatt gcttcagatc gtagctcaat
       61 caacggaatc accaaccagc tctacacaat tccaattgat gtcaccatag cgactctgat
      121 tccgttaccg atggcggcgg cagggtccta ctcaccagat gggtcccaac tcgcttatgt
      181 gccagttaca cctgcattta gagcatggaa acgctatcgc ggagggagag cctcatacat
      241 ctggatcgcc aacctggccg actccagcgt tgtcaaactc ccgcgtgaaa actcaaatga
      301 tttcaacccg atgtggattg gcgacaagat ttacttcctt tctgatcgca acggcccggt
      361 gactttgttt agctatgacc tcaagacaaa gaaagtcact caggaaatcc aaaattcggg
      421 gctggatttc aaagcagctt ctgccggccc ggatgtgatt gtgtatgaac aatttggatc
      481 gctccattat tttgaccttc aaacgggtaa aacccgcccg atgaacgtga caattgccgg
      541 ggactttctg gcagttcgtc cgaaatttga aaaagtcgct aatagcattc tcaatctggg
      601 catttctcca accggtgccc gggcagttgt tgaagctcgt ggtgaaatcc tgaccattcc
      661 gggtgaaaaa ggcgatgttc gcaacctgac gaacacgact ggtgttgccg aacgtgatcc
      721 atcctggtcg cccgacggca aatggattgc ctatttttca gatgaatccg gcgaatatgc
      781 cctccatctg cgtacccaga ccgggatggg cgaagtcaag aaaatcaatc tcggcacccc
      841 gtcgtcgttt ttttatactc cggtctggtc tccagatagc aagaaaattg ccttttgcga
      901 caagcggctc aatttctggt atgtcgaagt cgaaaaagga acgcccgtca aaattgacaa
      961 ttttgagcga gggctggtga ccaactacaa atggtcacct gacagtcagt ggctgaccta
     1021 cgccaagccg ctgaagtcgg tctataacgc agtgtttgtg tattcactgg ctgacggcaa
     1081 acttacccag atcaccgacg gcatgagcaa tgcggcctca ccagcgtttg atcgaaacgg
     1141 gaaatatctc tatttcacgg ccagcaccaa cagcggcccg cagatgtttg ggtttgacat
     1201 gtcaagctac ccacatcagc cgaccagcag cgtgtatgtg tgtgttctga aaaaagatga
     1261 ggcttcaccg ctcgcacctg aaagtgatga agaaaagcca gaaggtgccg ccaaggatgg
     1321 cgagaaaaag ccaggtgaag ccgacaaggg aaccggcaag cctgacgaga aaaaagaacc
     1381 acccaaggtc acgattgatg ttgaaaaaat caaccagcgc attttagcgc tgccaattcc
     1441 agcccgaaac ttcattgact tgtttactgg taaagacaat ctggtgtatc tggccgagtt
     1501 ttcgacggat gtgcaatcac cagggatcac cctgcacaag tttgatatgc aaaaacgcaa
     1561 acttgacaag gtgctcgatg gtgtcactgg ctggacgctc tcagccaatg gcgaaaaatt
     1621 gctctatggc caggggccgg cctggtttat cgccgcaatt gaccagccga ttaaacccgg
     1681 cgaaggtaaa ctcaaaaccg aggatctgac cgtttatgtt gacccacggg ctgaatggaa
     1741 tcagatgtac cgtgaaacat ggcgcattga acgcgatttc ttctatgacc ccaactacca
     1801 tgggcttgat atcaaagcaa ccgagaaaaa atatcaacct tacgtcaacg gcctcgtcca
     1861 ccgggctgat ttgaattatc tgttccagga aatgctcggt gaattgaccg tcgggcattt
     1921 gtatgtcagt ggtggtgata caccggatcc aaaacgtgtt ccaggtgggt tgcttggggc
     1981 tgattacacc attgaaaatg gccgctaccg gtttgcccgg gtgtttgacg gtgaaaactg
     2041 gaatccacaa ttgaaggcac ccttgaccca acctggcgtc aatgttgtgg ctggcgaata
     2101 tctactggcg gtcaacggtc gtgaattaac ggccaaagac aatgtctacg gtttctttga
     2161 aagcaccgca ggcaagcaag ttgtgatcaa agttggacca aaccctgacg gaactggtac
     2221 ccgtgaagtg accgtggttc ccgtagacag tgaagatggt cttcgcaatc tggcct
//
LOCUS       DAOQDO010000999         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43818_length_2275_cov_3.157207, whole genome shotgun sequence.
ACCESSION   DAOQDO010000999 DAOQDO010000000
VERSION     DAOQDO010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43818_length_2275_cov_3.157207"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKZ53_15170"
     CDS             <1..978
                     /locus_tag="PKZ53_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ41830.1"
                     /translation="ADLSRTNLEGANLENANLSRARLNASQLALANLGGARLDGAVLS
                     NADLQEAYLGGASMVKADFQNAVLEKANLEEADLTGANLRDASMRGALLPGANLTGVI
                     LERAVLADATLQRANFTKADCRHVTWAKAQLSGAIFNDALVYGGDLTADLAKMVKAEW
                     VDFSKDGDGSQRVKGSELVNYLNGLKEGGSAAAAVPAAAPVAPSTGNRRYFGRGDVLR
                     DAKLEFDTGSIVEVEGRFLKCSITLGDGAQLIIGKEGLLDGCRISGNGIIIVYGRFIE
                     DSAPGIDGAKQLIVGATGSVKGMIKQPPSLTQFAFEPGCFLRLKIATSS"
     gene            1134..1643
                     /locus_tag="PKZ53_15175"
     CDS             1134..1643
                     /locus_tag="PKZ53_15175"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ41831.1"
                     /translation="MSDQNTKRTIIEEGSELTGTLKSNCAVVVSGKVNGEVHSPSLVV
                     SEGGSVHGTIKVNDLKSQGEIAGQIDAEMVELSGKVNDQTIIRATMLEVKLSTPDSNK
                     LQVQFGNCSLEVGKEESSAPAAPAASGGDAQPQNRPQQGGGGQQGGGGHQGGGGGQQG
                     NQQKQKGRD"
     gene            1857..1930
                     /locus_tag="PKZ53_15180"
     tRNA            1857..1930
                     /locus_tag="PKZ53_15180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1891..1893,aa:Arg,seq:ccg)
     gene            1972..>2275
                     /locus_tag="PKZ53_15185"
     CDS             1972..>2275
                     /locus_tag="PKZ53_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ41832.1"
                     /translation="MTPTAYAVEMLEVTKRFGAVTANDRVTLRIEKQSIHAIVGENGA
                     GKSTAMNLLYGLYVPDSGEIRLHGETVALKTPRDAIARGLGMVHQHFMLVPSQTVAE"
BASE COUNT          565 a          571 c          622 g          517 t
ORIGIN      
        1 gccgatctgt cacgaacaaa cctggaaggc gccaatctgg aaaatgcgaa tttatcgcgg
       61 gccagattaa acgcctccca actggcactg gccaatctgg gaggcgcacg ccttgatggt
      121 gcggtcttaa gcaatgctga cctccaggaa gcctatttgg gtggcgcctc gatggtaaaa
      181 gctgatttcc aaaatgcagt tttggaaaaa gccaacctcg aagaagccga tctgacgggg
      241 gcgaatctcc gggatgcgtc aatgcgtggt gccttactgc ctggggcaaa tctgactggc
      301 gttattttgg agcgagccgt gctggctgac gcgacactcc agcgggcaaa tttcaccaaa
      361 gctgattgcc gacacgtcac ctgggccaaa gcccagttat caggtgctat ctttaatgat
      421 gctctggtat atggtggtga cttgacggct gatctggcaa agatggtcaa agccgagtgg
      481 gttgatttca gtaaagatgg cgatggttcg caacgagtca aaggcagcga actggtcaat
      541 tacctcaatg gtttgaaaga aggcggcagt gccgccgccg ctgtgcccgc cgccgcccct
      601 gtggcgccat caactggaaa ccgccgatac tttggtcgcg gcgacgtgtt acgcgacgcc
      661 aagcttgagt ttgacacggg ttcgattgtt gaagtcgaag gccgattcct gaaatgctcg
      721 attacgttgg gcgatggtgc ccagctcatc attgggaaag aaggcttgtt agatggttgc
      781 cggatttcag gaaatgggat catcattgtg tatggtcgtt ttattgaaga ctcagcgcct
      841 ggcattgatg gtgccaaaca gttgattgtc ggcgcgaccg gatcggtcaa ggggatgatc
      901 aaacagccac cttcgctgac acaatttgcc ttcgaaccag gttgcttcct gcggcttaag
      961 attgcaacct cctcgtaatc tccattccac ctgttgctta tctgcagggg ctgttcacac
     1021 gtcccctgcg gatcactccc attgccaaac acggttattt taacttcgaa ttatgaaaag
     1081 atgttggcag gcgcctggcg cttgacctta ctttcaagga gcatgtattc gccatgagtg
     1141 atcaaaatac caaacgcacc attattgaag aaggaagcga actgacaggg acgttaaagt
     1201 caaactgcgc cgtcgtggtc agtggaaaag tcaatggtga agtccattcg ccgtcgctgg
     1261 tggtcagtga aggcgggtcg gttcacggca ccatcaaagt caacgatctc aaatcgcagg
     1321 gtgaaattgc cggtcagatc gatgctgaaa tggtcgaact ctctggcaaa gtcaatgatc
     1381 agaccatcat tcgcgccacg atgcttgagg tcaaattaag caccccagat tcaaataaac
     1441 ttcaggttca gtttggcaat tgcagccttg aagttggaaa agaagaaagc agtgcccctg
     1501 ccgcaccggc ggccagtggt ggtgacgccc aaccccaaaa tcgtccacaa cagggcggtg
     1561 gtggtcagca gggtggcggt ggccatcaag gcggcggtgg cggccagcaa ggaaatcagc
     1621 aaaagcaaaa gggtcgcgac tgatcgtgcc atgtagaaat tgagttctgg tcagtgttca
     1681 agctttagcg agcaaaaacc ggtcgtttgt catattcaag ttattgatct gacttgagtt
     1741 gctagctttg tccgatgaaa gcaagctaaa gcctgaacgc tggacctgaa ccaaaacaaa
     1801 aattgaccac ttgaaatttc cagctcaagc aggtactctt cgcgctcaca aaatttgcac
     1861 ccttagctca gttggataga gcgttggcct ccggagccaa aggtcgctgg ttcgaatcca
     1921 gcagggtgta tccccacttt ccacatctcc aatttcgagt tcacaaatcc aatgactccc
     1981 acagcatacg ccgtcgagat gcttgaggtg accaagcgtt tcggcgccgt cacggccaat
     2041 gatcgggtca cactccgcat tgaaaagcaa agcattcacg ctattgtggg agaaaatggc
     2101 gccggcaagt ccaccgccat gaatctgttg tatggcctct atgtgcctga cagcggtgaa
     2161 atccggctgc acggagagac tgtcgccctc aaaaccccac gggatgcaat tgcacgcgga
     2221 cttggcatgg ttcaccagca ctttatgttg gtgccttcgc agacggtggc cgaaa
//
LOCUS       DAOQDO010001000         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43821_length_2275_cov_2.914414, whole genome shotgun sequence.
ACCESSION   DAOQDO010001000 DAOQDO010000000
VERSION     DAOQDO010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43821_length_2275_cov_2.914414"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..2258)
                     /locus_tag="PKZ53_15190"
     CDS             complement(57..2258)
                     /locus_tag="PKZ53_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41833.1"
                     /translation="MRFTLRTLLLLILISTLVLPGVMLTQSPVKASRQAGPTLLKRTV
                     GITLKKYLRFWKNPQAAEPQYDTWSWVPRIDFEMIGPIPEGSQVLVDFSFPDGKPWMQ
                     YPLRTEELPEDGWGKFSSYDLPISDSDLEKKATIQTGAFPFKIILKNSLLGSNDVLFA
                     GKFKVATFTPDQKIPEFKNKQEFYIQEDWRLPFGYIWLDPSSDENVPTFKTQFWIRNA
                     DNYAKMEAFLYYKGKQVGETQSNEVESELTTAVDDKAYKWTLRTFPFLKVRGFNHDTS
                     SANDYSALFFLDKNPGEYELKILRNSKLDRIAKFTVGADGKIVDNGIAKANKLGGVRM
                     VLPVAIVSDADGKWDRAAWKTDALYGNPLSGFTAIEGGAAPETGSTASASTSGTSAAS
                     SETTAAPASGGPTLLKYSLHVRARRMLRYWKFPDQDNYWSWIPEVEFQVLGPVAGGSQ
                     FQVDFTLPDGKPWTTLNCNSDAVAAGQVWKFNTEWERGHDDKRAITDAGTFGFKIRLK
                     NELSNLNYEVFSGKFNVNKFHVGNELPQFKNQFEYYVDQDWNLPIGYLWLNIFASQDA
                     PPLVATMWFRGENDDTRLAGYVLYNGKIIASTKDTTSGVAGYDKAILTSGNDADPRWE
                     RWNFTWNKVRGWKLESPTEYPGAHFLSVNPGEYEIKVLNQGELVRQAKFTVGADGRIV
                     DNGIATQNKLGGISMVLPVKVLGPPTIKMDLNAWKTDAFYKNPLTGFTAPQ"
BASE COUNT          544 a          589 c          516 g          626 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattcttc attccactac
       61 tggggtgcgg taaatccagt taatggattt ttgtaaaagg catcggtttt ccaggcattg
      121 agatccattt tgatcgtggg tggtcctaaa accttcacag gtaaaaccat gctgataccg
      181 ccgagtttgt tctgagtggc gatgccgttg tcaacgattc ggccatctgc tccaaccgtg
      241 aatttggcct gccggactaa ctcgccttga ttcaacacct tgatttcata ttcaccaggg
      301 ttgactgata ggaaatgtgc gccaggatat tcggttggac tttccagttt ccagccgcgc
      361 actttgttcc aggtgaaatt ccagcgttcc cagcgtgggt cggcatcatt gccagacgtc
      421 aagatcgctt tatcataacc ggccacgcca gatgttgtgt ctttggtgct ggcaataatc
      481 ttgccgttat agagcacata acctgccagc ctggtgtcgt cgttttcgcc gcgaaaccac
      541 atggtggcca ccagcggcgg cgcgtcctga ctggcaaaaa tattgagcca gagatagcca
      601 atcggtaggt tccaatcctg gtcaacataa tactcaaact ggtttttgaa ttgcggcaac
      661 tcattgccta cgtgaaactt gttgacatta aactttccag aaaacacttc atagttcaaa
      721 ttgctcaact catttttgag tcggatttta aacccaaatg ttccagcgtc agtaatcgcg
      781 cgtttgtcat cgtggccacg ttcccattcg gtgttgaatt tccacacctg cccggcggcc
      841 acggcatcag aattacaatt gagcgtcgtc cagggctttc catcgggtaa cgtgaaatca
      901 acctggaact gacttccacc cgccaccggg cctaacacct gaaactcaac ttcgggaatc
      961 cagctccagt aattgtcctg atctgggaat ttccagtacc gcagcattcg acgggcacga
     1021 acgtggagcg aatatttcaa caaggttggt cctccacttg ccggcgcggc tgtggtttca
     1081 gatgaggcgg ctgaagttcc cgaagtgctc gccgaagccg ttgaacctgt ttctggcgcg
     1141 gcgccacctt caattgccgt gaacccactc aacgggttgc catagagcgc atctgttttc
     1201 caggcggcgc gatcccactt tccatcggca tcgctcacaa ttgcgaccgg gagcaccatc
     1261 cgaacaccac ccagtttatt ggctttggca atgccgttat ccacaatttt tccatcggca
     1321 ccgacggtaa atttcgcgat tcgatccagc ttgctgttgc gcaggatttt taattcgtac
     1381 tcgccagggt tcttatcgag gaaaaacagg gctgaataat cattggcaga agaggtgtca
     1441 tggttaaacc cacgaacctt cagaaatgga aacgttcgta aggtccactt gtaggcttta
     1501 tcatcaacag cggtggttaa ttcgctttca acctcgttac tttgggtttc cccaacctgt
     1561 ttgcctttgt agtacaaaaa agcttccatt ttggcgtagt tatcagcatt gcgaatccag
     1621 aattgagttt taaaggttgg tacattttcg tcagaactcg gatccagcca gatataccca
     1681 aagggcagtc gccagtcttc ctgaatataa aattcctgct tgtttttgaa ttcggggatt
     1741 ttctggtctg gcgtaaacgt ggcaacctta aattttccag caaacagcac atcgttgctt
     1801 ccaagcagcg aatttttcaa tatgatctta aatggaaagg cccccgtctg aatggtcgcc
     1861 tttttttcca gatcagaatc ggaaattgga agatcgtagc tggaaaactt gccccatcca
     1921 tcttcgggga gttcttcagt ccgaagcgga tactgcatcc agggcttgcc atctggaaaa
     1981 ctgaaatcca ccagcacctg acttccttca ggaatgggcc caatcatttc aaaatcaatc
     2041 cgcggcaccc acgaccaggt atcatattgc ggttcagccg cctgcgggtt tttccaaaag
     2101 cgtaaatatt tttttaatgt tatgccgacg gtccgcttaa gcagcgttgg accagcctga
     2161 cggctggctt tcaccggaga ctgagtcaac ataacccctg gcagcaccag ggtcgaaatc
     2221 aatatcaata agaggagcgt tcgaagagtg aatcgcatag cagtcctttt cattc
//
LOCUS       DAOQDO010001001         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43898_length_2273_cov_2.671776, whole genome shotgun sequence.
ACCESSION   DAOQDO010001001 DAOQDO010000000
VERSION     DAOQDO010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43898_length_2273_cov_2.671776"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKZ53_15195"
     CDS             complement(<1..403)
                     /locus_tag="PKZ53_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41834.1"
                     /translation="MLNKIQFNPCVEAGSETSSGSSASGSSRVIQIFSLCTFVLLVCG
                     ICALTAAPTSSAAARVKSKRTLAKAPRRMVALPQEEKKGANLTEVDDNIPLSGIPVSG
                     TYRAFSTTGPYVYVADTSNNRIQRYDGVTWET"
     gene            1063..2151
                     /gene="tsaD"
                     /locus_tag="PKZ53_15200"
     CDS             1063..2151
                     /gene="tsaD"
                     /locus_tag="PKZ53_15200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNJ41835.1"
                     /translation="MTFRYVLGIESSCDETAASVLEHGTVVRSNIIASQIETHRPFGG
                     VVPEVASREHLKRICDVVEQALTQAEIEASQLDGIAVTCGPGLIGSLLVGVSYAKALA
                     YALQKPLIGIHHIEGHIYSVVFEHPPIQYPALALIVSGGHTNLFWIPEENHYIRLGHT
                     RDDAAGEAYDKTAKLLGLGYPGGPVIDRLAKTGVASEAPLTYRPPHMPDAPYDFSFSG
                     LKTAVLRFVQEHHLGRLAEGEEVPAIIRNLSASFQKAVVTSLTRNLLRAARDYPPKTI
                     ILAGGVACNSALREAMKDVSKTVGVPVAFPSPVFTTDNAAMIAAAGYVRLQRGETSDL
                     KLAAEPTLKLHSEDASPAPSRGKKRFRS"
BASE COUNT          566 a          543 c          607 g          557 t
ORIGIN      
        1 atgtttccca ggtcacgcca tcatagcgct gaattcgatt atttgaggta tcagccacat
       61 agacatacgg gccagtggtc gaaaaagccc gataggtacc actgaccgga atgcctgata
      121 aaggaatgtt gtcgtcaacc tcggtcaggt tggcaccctt tttctcttct tgtggcaaag
      181 caaccatgcg tctgggagct ttggccaggg tgcgtttgga tttgactctg gcggctgccg
      241 atgaggtggg tgcagccgtc agcgcacaga tcccgcacac cagaagcaca aacgtacaca
      301 aggaaaaaat ttggatgacc cggcttgatc cagatgccga agagcctgaa gaggtttcag
      361 atcctgcttc gacgcaaggg ttgaattgaa ttttgttgag cataaagttc tcctctgagt
      421 tcactcactt ttcgtgtctt ttaaagtttc aagttatcaa tagtgcaggt taccctggat
      481 ttatttccct tttttaaagc gaagttttca ggctctcacc tcagagtgta tctaaccgtc
      541 aatgcagaat tatggcttgt gatttagtgg ctcaatgcaa ggtgccaacc aagttgtttt
      601 tttgtgattg atgaccaaac caagcggcat ttcaaagttt gagtgaaatt ttgttgccgc
      661 ccagaagtgc aagctggtta tgtacttctg aagaagtttg aaaaaaccct ggtttgaaaa
      721 aattgaactg accggctgaa gggatgagga tcaggtccga tttcactggg gacaaaatac
      781 ttccacagac aaacctgata ctgaaacaat acttgatacc cgtacccggt acaagctgaa
      841 ttttccatct tcctggcagg aaaaatctgt aaaaagcggt taacttttca tctcaggtga
      901 gtctagttgt gtttctggat tgaattgaaa cttcctgaag tggctggggt tggggttggg
      961 aggggggtga cctcagcaac tcgtcgttga ttaaagctga ctggcgccag tactcttcag
     1021 gttttggccc agaactttac aaaggatcct tgttttacac ccatgacatt tcggtatgtg
     1081 ctcgggattg aaagttcctg tgacgaaacg gcagcgtccg tccttgaaca tggcaccgtg
     1141 gtgcgctcta acattattgc ttcgcaaatc gaaacccatc gcccatttgg cggggtggta
     1201 ccagaggtgg catcacgcga acatctcaaa cgaatttgtg atgtggtcga gcaggcgctc
     1261 acccaggctg aaattgaagc cagccagctc gacggaattg ccgtgacctg tggcccaggc
     1321 ttgatcggat cgctcctggt cggtgtgtcc tacgccaaag ccctggccta tgcgctccag
     1381 aaaccgctca tcggcattca ccacatcgaa ggtcatatct attccgtcgt ttttgaacac
     1441 ccaccgattc agtatccggc cctggcattg attgtttcgg ggggacacac caatttgttc
     1501 tggattccgg aagaaaacca ttacatcagg ctgggccata cccgagacga cgcggcgggt
     1561 gaagcctatg acaaaacggc gaaattgttg gggttgggat atccaggagg accggtgatt
     1621 gatcggctgg ccaaaaccgg cgtcgccagt gaagctccgt tgacctatcg cccgccacat
     1681 atgcccgatg ccccctatga tttctcattt agcggactca aaaccgcggt gttgcggttt
     1741 gtgcaggaac accatcttgg ccgacttgcc gaaggtgagg aagttccggc aatcattcga
     1801 aatctctcag ccagtttcca aaaggcagtg gtcacgtcgc tgaccagaaa tctcctgcgt
     1861 gcagcacgag attatcctcc gaaaaccatt attttggcgg gtggggtggc ctgtaactcg
     1921 gcgttgcggg aagcaatgaa ggacgtttcc aaaacggtag gggtgccggt ggcgtttccg
     1981 agtccggttt ttaccacgga taatgccgca atgattgcgg ctgccggcta tgtgagatta
     2041 cagcgagggg aaaccagcga tttaaagctg gccgccgagc cgacattgaa actgcattct
     2101 gaagatgcca gcccggcgcc cagccggggg aaaaaacgat tccgaagctg aaaaccctcg
     2161 ggctgaagac aacgggctga agaaaacggg ctgaagacaa cgggctgaag acaacgggct
     2221 gaagacaacg ggctgaagaa aacgggctca gggtattaaa cctattttct tca
//
LOCUS       DAOQDO010001002         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43920_length_2272_cov_3.480830, whole genome shotgun sequence.
ACCESSION   DAOQDO010001002 DAOQDO010000000
VERSION     DAOQDO010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43920_length_2272_cov_3.480830"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKZ53_15205"
     CDS             complement(<1..1007)
                     /locus_tag="PKZ53_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41836.1"
                     /translation="MNYQKPPFTKENEPNRKLSWYELKEMLFQAIKKQFRYVSPPVKS
                     QIKRLSPAQLESVLDILPQFSDSQYLETHLTEVLSKPTPARNRDRFNPPGPLPERQFN
                     REITPPTQLPQMDEGPEDTEPKEAESEPFPTRVVDGPVEFQSASEPVDDSVAFIPEPV
                     QSLPVPEPAESYQPYTQRQPVRPVAPPSRMTPPPPPPPPSGVMRSAVPIVIEGKQLEL
                     VLEIDRFSDEKQKRGVRFSFSMDETQFLVAQLLTHRQHIHPLEPRVAEAIRSYFNEAE
                     QVLQGALQEVQTPAVAQPAAVRQPVEVLAPAPEVEPLVEVSPESVSQPDPVQVNEPVV
                     S"
     gene            1733..>2272
                     /locus_tag="PKZ53_15210"
     CDS             1733..>2272
                     /locus_tag="PKZ53_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HNJ41837.1"
                     /translation="MPFLKHLVACFILLLGLTTGISAQIPTILNSGTTPTGKVIKTST
                     RTIVIPPEKVEPVRIPFFESIPTIDGNLDDPVWNTAAVLKGFFQINPGDNIEPSRDTV
                     VRVGYDAKHLYFGFYCYDEPDKVRATVAQRDTVFNEDNIRVFLDTFNDQRRAYVLGFN
                     PFGIQQDGILTNGGDTDYSI"
BASE COUNT          549 a          517 c          588 g          618 t
ORIGIN      
        1 cttgatacca ctggttcatt cacctgaacc ggatctggct gggagactga ctctggtgag
       61 acttcaacaa gcggttcaac ctctggggca ggagccaaaa cttcaaccgg ttgacgtacg
      121 gcggctggtt gagccacggc cggtgtctga acttcctgga gtgctccctg aagcacctgt
      181 tcggcctcgt taaaatagga ccgaattgct tcggcaacac gaggttcgag tgggtgaata
      241 tgctggcgat gggtcaacaa ttgggcgacc agaaactggg tttcatccat tgagaatgaa
      301 aagcgtaccc cgcgtttttg tttttcgtca gagaaccggt caatttcaag caccagttca
      361 agctgctttc cttcaatcac aatggggacg gctgaccgca tcacgcctga tggcggcggt
      421 ggcggcggcg gtggggtcat ccggctgggc ggtgccacgg gcctgactgg ttgccgttgt
      481 gtatagggtt ggtaactttc agcgggctct ggtactggca ggctctgaac cggctcagga
      541 ataaaggcaa ctgagtcgtc cactggttca ctggctgact ggaactcaac tggtccatca
      601 acgacccggg ttggaaatgg ctctgactct gcttccttgg gttcggtatc ctctggcccc
      661 tcgtccattt gaggaagctg agttggcggt gtgatttcac ggttgaattg ccgttccggt
      721 aatggtcctg gtgggttaaa ccgatcccgg ttgcgggccg gcgtcggttt actcaagacc
      781 tcggtcagat gggtttccag gtattggctg tcagaaaatt ggggcaaaat atccagtact
      841 gattccaact gcgccgggga taaccgtttt atctggcttt tcaccggagg cgacacatag
      901 cgaaattgct ttttgatcgc ctgaaacagc atttccttga gttcatacca ggagagtttt
      961 cggtttggtt cattctcttt cgtgaaaggt ggtttttgat aattcatttg aagtggattt
     1021 ggaattggaa agaagtgatt ggggggaaca agaggaacca tgaattatga gttatgagtt
     1081 tcccatctga ttgaaccttt gatggttagc tcttcctttg atcatacttc attcctcgat
     1141 gatgcatatc tcggcccttt atttcggtgg aatgtctcct tgaaataaag aagcctgggt
     1201 tatgaaccat tttcagcaaa aagaaaagca aattccggcc tgattgatgt gaaatctaac
     1261 actttggtca aatcatcagc ggcactcagt cccaaggggg ccgggaaaac tctagctttt
     1321 cagaagagga aaaaagttcc gaagtaacgc cttcagatgc aaaaagtatc gggtggagtg
     1381 gaagaaaaat ctttcgccca aagacgatag acagaacctt tttctgaaaa gttatatctg
     1441 gattttacac cacggaaaat tcgaagttcg tcctctgtgt cagggaagtg agcctgggtt
     1501 tatatggtgt gccaggggat ccagtcgcct ggttttttga gtcgtgggtg gtggttttga
     1561 gattgaaggg aagtaaaaaa aggaggtttt cctgctttct atcctgtgtc atactcccaa
     1621 cccaagacca aactcctttc tttcaaatga ttcctgtgta tggcgttaaa atttctgtga
     1681 gcactggaat cgggcgacat cccattcatc agattttcct tgagacagcc ttatgccatt
     1741 tctgaaacac ctggttgcct gcttcattct gttactcggt ttgaccaccg gcatatcggc
     1801 tcaaatccct acgattctca atagcggaac cactcccacc gggaaagtga tcaaaacctc
     1861 aacccgaacc attgtcattc cccctgaaaa agtcgagccc gtgagaattc cattctttga
     1921 gtcaataccg acgattgatg gaaaccttga cgacccagtt tggaacactg ccgcagtgct
     1981 caaaggattt ttccagatca accccggaga taatatcgag ccttcgcgcg ataccgttgt
     2041 tcgggttggg tatgacgcca aacacttata ctttgggttc tactgttacg atgaaccaga
     2101 caaagttcgg gcgacggtgg cacaacgcga cacggttttt aacgaagaca atattcgggt
     2161 atttttagat accttcaatg atcaacgccg ggcctatgtg cttggattta atccatttgg
     2221 aattcagcaa gacggaattt tgaccaatgg cggtgatacg gactacagca tt
//
LOCUS       DAOQDO010001003         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43923_length_2272_cov_3.161479, whole genome shotgun sequence.
ACCESSION   DAOQDO010001003 DAOQDO010000000
VERSION     DAOQDO010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43923_length_2272_cov_3.161479"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKZ53_15215"
     CDS             complement(<1..446)
                     /locus_tag="PKZ53_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41838.1"
                     /translation="MGWKTDWLYSLIFAEVRHLWNRARRESERMGPDFGPAPGTLIIS
                     EYSDGNFTNATCRGLRKRQWRYSGDEQLTLIEPDDPDISHVSETSDQMFFSVGEVRFS
                     ISADRKSLIFEYALGPRYGCGQVFQVHGQGKHGRLGQVEHSISWRA"
     gene            complement(506..946)
                     /locus_tag="PKZ53_15220"
     CDS             complement(506..946)
                     /locus_tag="PKZ53_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ41839.1"
                     /translation="MTDLNKNQPNQNQPSLPNGYVPPASVEELHKRYAAGERYFMGAD
                     IQGGANLQWANLEGANFECAILSDIDFRSANLQRVVFRNANVKCSDFRGANLEGADFG
                     GANVEATQFEGANLSSTNFKGASYYGHILKAEDVPEWIKMAESE"
     gene            complement(1134..2093)
                     /locus_tag="PKZ53_15225"
     CDS             complement(1134..2093)
                     /locus_tag="PKZ53_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41840.1"
                     /translation="MQRKSLWLMVSLCLCLGMVWFPLDTQAQKKRLIAHEWGTFTTHV
                     DVNGVVQTWNPLNAPRDLPGFVRVYHPKNGIQGTVRMETPVIYFYTSKKMTVSVDVEF
                     PNGQISEFYPDTSFRGHGIGWKNCLIDPTAEPNFLKESEESHYYPARETDAVPVRVKK
                     ASTGEQLEKFLFYRGVGTFDLPLGVRLDGNQFVVKNNGTEPIAKVAVFENREGKAGFA
                     IRDLATEELRIERPALETQSLEVFKTEIETLLVANGLYEKEAKAMIKTWNDVWTAEGL
                     RVFYLIPRQTTDKILPLKITPAPAELVRVMVGRVEVQEGTKME"
BASE COUNT          554 a          579 c          531 g          608 t
ORIGIN      
        1 tatgcccgcc acgaaattga atgctcaacc tgaccaaggc gtccatgttt tccctgaccg
       61 tgaacttgaa acacctgccc acagccatag cgtgggccaa gtgcatactc aaaaatcaga
      121 gacttgcgat ctgcgctgat agagaatctg acttctccaa cagagaaaaa catttggtca
      181 gaagtttctg aaacgtgact gatgtctggg tcatctggtt caatcaaagt cagttgttca
      241 tcaccagagt atcgccattg ccgttttcga agtccccggc aggttgcgtt tgtgaaattg
      301 ccgtcagaat attctgagat gatcaaggtg cctggggccg ggccaaagtc agggcccatc
      361 cgttcactct cccgccgggc acgattccac aggtgacgaa cttcagcaaa aataagtgaa
      421 tacagccagt cggttttcca gcccatacaa ttttgacttg agagtcacga tttcccctat
      481 ggatccagtt tccagtgaaa ccagatcatt cgctctcagc catctttatc cattccggta
      541 catcctcagc cttgagaata tgtccataat aacttgcccc tttgaagttg gtgctgctca
      601 ggttagcacc ttcaaactgt gtagcttcca cattggcacc cccaaaatca gcaccttcca
      661 ggtttgctcc ccgaaaatct gagcacttca cattggcatt tctgaacaca accctttgta
      721 aattggctga tcggaaatct atatccgata agatggcaca ttcgaagttt gctccctcaa
      781 gattggccca ttgaagatta gccccaccct gtatatcagc acccatgaaa tatcgctctc
      841 cggcagcata ccttttgtga agctcttcaa cagaagcagg gggaacatag ccgtttggta
      901 aagaaggttg attctgattt ggctgatttt tgttcaagtc agtcatactt ttaaaggttt
      961 aggcttcatt cccaaaattt cgcccaaaat accgactatt tcctcgggtt tgacctcaaa
     1021 tgtggttgat tgggtccgaa ccaaaggaca taaaggacat aaaagacagg ctctggaagt
     1081 caatttcggg gaagctgtgc tcccattcct gaccttggag attaagtcat tctttattcc
     1141 atctttgtcc cttcctgaac ttcgaccctt ccgaccatca cgcgaaccag ttcagcgggc
     1201 gcgggggtga ttttcagtgg cagaatcttg tcggttgtct ggcggggaat cagatagaaa
     1261 acccgcagcc cttcggcggt ccagacgtca ttccaggttt tgatcatggc tttggcttct
     1321 ttttcataca aaccatttgc caccagaagc gtctcgattt ctgttttaaa tacctccaat
     1381 gactgggttt ccagtgcagg gcgttcgatt cgaagctcct ccgtagccag atcgcggatc
     1441 gcaaatccgg cttttccttc ccggttttca aagacggcaa ctttggcaat cggctcggtg
     1501 ccattgtttt tcacgacaaa ctggttgcca tcaagtcgca cgccaagggg caaatcaaag
     1561 gtgccgacac cacggtaaaa gaggaatttt tcaagctgtt cgccagtgct cgcttttttc
     1621 actcggacgg gcaccgcgtc ggtttcacgg gccgggtaat agtgactttc ttccgattct
     1681 ttcaagaagt ttggctcagc cgttgggtca atcaggcaat tcttccaacc aatgccgtgc
     1741 ccacgaaagc tggtgtctgg ataaaattcg ctgatctggc cattggggaa ctcgacatcc
     1801 acggaaacag tcattttctt ggaggtgtaa aagtaaatca ctggtgtttc catccgtacc
     1861 gttccctgga taccgttttt cggatgatag actcgcacaa atcccggtaa gtcgcgtgga
     1921 gcattgagcg gattccaggt ttgaacgaca ccattgacgt ctacgtgtgt ggtaaatgtg
     1981 ccccactcgt gggcaatcag tcgctttttc tgtgcctgag tatcaagcgg gaaccacacc
     2041 attcccaaac aaaggcacag ggaaaccatc aaccagaggc ttttgcgttg cataggtgaa
     2101 acttcctttc agcaggtgtc ggcctggaac tgacgttaac cgattgtggg aaaatcagat
     2161 ggagattccg gagaggaatc cgattgaatg tcgcgtttga tgccgtggct gggcttcaac
     2221 gtttgcggtg ccacaggttg agcacgaaat ttttcaactg gaccttcgac gg
//
LOCUS       DAOQDO010001004         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43925_length_2272_cov_2.915201, whole genome shotgun sequence.
ACCESSION   DAOQDO010001004 DAOQDO010000000
VERSION     DAOQDO010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43925_length_2272_cov_2.915201"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1814
                     /locus_tag="PKZ53_15230"
     CDS             180..1814
                     /locus_tag="PKZ53_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41841.1"
                     /translation="MALLGAIRPGTIAGLIRLLSQGEAVATLICERGPTRVDFHFSNG
                     KIESMEVNQLPSERGLDIVTTWETGVYRLIKGRIERGYGLFESSDRGAGARGHVLILS
                     ADVSLTGMAENTLEQIGYQVSVAPSSAQAVETLAQISPTLIILDTEPGDSELTAAEQL
                     RDHDHGRDLPIIALTRMPDASLARSLHLLGLSSITKPVSPEALVSVVQQEIQMYHMRS
                     SRDWSALAVPAETPEIAADIVDLLLFEEVIPSLDSIFDKSSKLGIKIALGEASPETVA
                     LFDLFDGRNSLREIIAANPKLGQKVKFLASYMVKTGLLETVAEAPKTQTVIDEETGEI
                     SEVPLPSLAKPAPRERPDQPYFTAVETLDLADALHSTYGPDAQWAKLLSLGLPPAYRG
                     AFADTLRFISSVQSNFIKRGASVAIPALPEYEVARVLMRSDLLMSIHDVEDESRLMDV
                     TRLLGGNIWGFVFFVSMHQRSAMTYLRSLVHSVTRSYQRPYVVNLLNVGASVEADAIQ
                     LVEDTLEIDRKGVVGVWNIQKPEAVIKMIDQLIQLS"
     gene            1919..2161
                     /locus_tag="PKZ53_15235"
     CDS             1919..2161
                     /locus_tag="PKZ53_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41842.1"
                     /translation="MSENLVPTPPIDAIVWSKEVEVRGLSLPLQLEYEKRGAFWATRG
                     LRMTDGLFCQLEREHPELTFVKREWIAPKKKDDKAE"
BASE COUNT          548 a          553 c          589 g          582 t
ORIGIN      
        1 tttattttct gtgtgttccg tgtattccgt ggtttcttta tctgaaattc tatagcgcga
       61 aagagtgatg gagtgaaaga caacgagttt agggatttac agagcttaat ctgagacgca
      121 acaccaggct acttcgctac ttcgctgatt tgcttaaatc cttcaaaggt taaactgcta
      181 tggccttact gggtgccatc cgtccaggta caatcgcagg gttaattcgc ctcttgtcgc
      241 agggagaagc cgtcgccacg ttgatttgcg agcgcggccc gacacgggtc gattttcatt
      301 tcagcaatgg caaaattgag agcatggaag tgaatcagct cccgagtgag cgggggctcg
      361 atattgtcac cacctgggaa accggtgtgt atcgcttgat caaaggtcgc attgaacgcg
      421 gatacggact ctttgaatcc agtgatcgtg gggccggggc ccggggacac gtgttgatct
      481 tatcggcgga tgtcagcctg acaggaatgg ctgaaaacac actcgaacaa attggatacc
      541 aggtcagtgt ggcgccaagt tcagctcagg cggttgaaac cctggcccaa atttccccaa
      601 ccctgatcat tctggatacc gaacctggag acagcgagtt aaccgcggct gaacaactcc
      661 gtgaccacga tcatggtcgg gacttgccaa tcattgccct gacccgaatg ccagatgcct
      721 ctctggcacg ctcgctccat cttttgggat tgtcttccat taccaagccc gtttcccctg
      781 aagccctggt gagtgtggta cagcaggaaa tccagatgta tcacatgcga agctcgcgtg
      841 actggtcggc actggcggtt ccggctgaaa cacctgagat tgcggctgat attgttgacc
      901 tgttactgtt tgaagaagtc attccgtcac tggacagcat ttttgacaaa tcctccaaac
      961 ttggaatcaa aattgccctt ggcgaagcgt caccagagac ggtggcctta ttcgatttat
     1021 ttgatggacg caattcgctc cgggaaatta ttgcagccaa tccaaagctc gggcaaaaag
     1081 tcaaattttt agcctcgtac atggtcaaaa ccgggttact tgaaaccgtg gctgaggcgc
     1141 ccaagacgca gacggtgatt gatgaagaga ccggagagat ttctgaggtt cctctgccaa
     1201 gtctggccaa gcccgcacct cgcgagcgac ctgaccagcc gtactttacg gcggtggaaa
     1261 cgctggatct ggcagatgcc ttgcactcaa cctatggacc tgatgcccag tgggcgaaat
     1321 tactttcgct tggtttgccg ccagcttatc gaggggcatt tgctgatacc ctgcgcttta
     1381 tttcctcggt tcaatcaaac ttcatcaagc ggggagcctc cgtggcgata ccggctttgc
     1441 ctgagtatga ggtcgcccgg gttttgatgc gatctgattt gttgatgagc attcatgacg
     1501 ttgaagatga aagccggttg atggatgtga cacggctgtt aggtggcaat atttggggct
     1561 ttgtcttttt tgtcagcatg catcagcgtt cggccatgac ctatcttcgt tcactggtgc
     1621 attccgtcac ccgaagttac cagcgaccct atgtggttaa ccttctcaac gttggcgcct
     1681 cggttgaggc cgatgccatt caactggttg aggataccct tgaaattgac cgcaagggtg
     1741 tcgtcggcgt ctggaatatc caaaaaccag aagctgtgat aaaaatgatc gatcagttga
     1801 ttcaactcag ctaaccattt caaatccaat ctgaaaaaga gcatcaaacg acgttcactc
     1861 tcaccccctt gttgagtgat tccaggtagc ttccgtttga tgaatacaat ctttctatat
     1921 gtctgaaaac cttgttccta ccccacccat tgatgccatt gtatggagta aagaagtcga
     1981 agtgcgaggc ttgtcgcttc cactccagct tgaatatgaa aagcgtggcg ccttttgggc
     2041 cactcgtggg ttgcgcatga ccgacgggct cttctgccag cttgagagag agcacccgga
     2101 actcacattt gtcaaacgcg aatggattgc accgaagaaa aaggatgata aggcagaatg
     2161 aagaatgtgc ttggtgctta gtgcttggtg cttggtgctt ggtgcttggt ccttagaaat
     2221 caatattttc gaagaagaac caagaaccaa gaaccaagaa ccaagaacca ag
//
LOCUS       DAOQDO010001005         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43935_length_2272_cov_2.582318, whole genome shotgun sequence.
ACCESSION   DAOQDO010001005 DAOQDO010000000
VERSION     DAOQDO010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43935_length_2272_cov_2.582318"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..387
                     /gene="yajC"
                     /locus_tag="PKZ53_15240"
     CDS             70..387
                     /gene="yajC"
                     /locus_tag="PKZ53_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNJ41843.1"
                     /translation="MHLDLLLFLQSGSGSAFSGLLVPVIILGIFFLLVILPQRKQQQK
                     HQEMLDNLKSGDKVIALGGLIGTVMSVSTKDNTVQLQIAPSVRVEVVRNAITALQQEK
                     SEK"
     gene            483..2165
                     /gene="secD"
                     /locus_tag="PKZ53_15245"
     CDS             483..2165
                     /gene="secD"
                     /locus_tag="PKZ53_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNJ41844.1"
                     /translation="MRKNLLQRALLILSVTGICLYLTLIGYRQPTMKDFTSWSQVKTN
                     LANNLKLGLDLKGGSQLLMQVRAEEVIAQVTTNNVEAAKTRLQKKGIAVANASSARPG
                     QVSVTISDAARIQEAEKELRLDLDKQEWASSISGSTITFTLNETAAEAIREQSVTQAM
                     TIIDNRVNAFGVAEQSLQRYGRQNTYQILLQLPGVDDPERVKKTLVLESKLELRPTDS
                     PFQTYPSLDEAQKAAAGKKDSEGNEFEPLPYDERGDDETEAATPPATPDPAKPAVYVL
                     IAKKNVITGGDIRDALALPADAAGHDYKINFRLKQAGAEKFKTWTGANIGKNLAIVLN
                     NRVKSAPSIRSEISDSGEITGNFTKQSGEDLALVLRSGALPARVEYIEERTVGPSLGA
                     DSIRSGVLASVAGLIFIMIFMGIYYQFSGINAIVSLILNLIMLIAALAMFSATLTLPG
                     IAGIILTIGMAVDSNVLIFERIREELKLGKAVVSAVETGFAKAFITIIDTHVTTIVSS
                     VFLFIFGTGPIRGFAITLIIGLLANLFTATFVSRTMFGYTLSRKERVTSLSI"
BASE COUNT          552 a          567 c          574 g          579 t
ORIGIN      
        1 caagttttga gcaaagcgcc cctgcgccgg catggagctt tgctctgcgg tgtttctttt
       61 tgaactgcaa tgcatttaga ccttcttttg ttccttcaat caggtagcgg tagtgctttc
      121 agcgggttat tagtgcccgt catcatcctg ggtattttct ttctgctcgt tattctgccg
      181 caacgcaaac aacagcagaa acaccaggaa atgctcgaca acctcaaaag tggtgacaaa
      241 gtgattgccc tgggtgggtt gattggtacg gtgatgagtg ttagcaccaa ggacaataca
      301 gttcaattgc aaattgctcc ttcggtgcgg gttgaggttg ttcgaaatgc catcactgcc
      361 ctgcagcaag agaaatccga gaaataagct tcgattttta gattcatgat ttcaggttga
      421 ctactgtcct gatcagggct ggtttgacca aaatcgtgga cgttttcatg aggattgaga
      481 gaatgcgtaa gaatttgctc cagcgtgccc tccttatctt atctgtcaca ggtatctgtc
      541 tatacctgac actgattggc tatcgccagc caacgatgaa ggattttacc tcctggtcac
      601 aggtcaaaac caacctggca aacaacctca aacttggact tgacctcaag ggtgggtccc
      661 agttgttgat gcaggtccgg gctgaagaag tcattgccca ggttaccacc aacaacgttg
      721 aagccgccaa aacccggctt caaaagaagg gaattgcggt tgccaacgcc agcagtgccc
      781 gaccgggcca ggtgagtgtc accatttcgg atgcggctcg gattcaagaa gccgaaaaag
      841 aactccgcct ggatcttgat aaacaggaat gggcttcttc catcagcggc agtaccatta
      901 cctttactct caatgagacc gcggctgagg ccatccgtga gcaatccgtg acccaggcca
      961 tgacgattat tgataatcgt gtcaacgcct ttggggtggc agagcaaagc ttacaacgct
     1021 atggtcggca aaacacgtac cagattctgt tgcaattgcc tggggtggat gacccggaac
     1081 gtgtgaaaaa gacgctggtt ctggaatcca aacttgaact ccgaccaact gattcaccat
     1141 tccagaccta tccctcactt gacgaagctc aaaaggcggc ggctgggaaa aaagatagtg
     1201 aagggaatga atttgaacca ctgccctatg acgaacgggg cgatgatgaa actgaagccg
     1261 ccacaccgcc tgcgacccct gaccccgcca aacccgccgt ctatgttttg attgcaaaga
     1321 aaaatgtgat tacgggtggt gatattcgtg acgcactggc acttccagcc gatgcggctg
     1381 gccatgatta caaaatcaat ttccggttaa agcaggctgg tgcggaaaag ttcaaaacct
     1441 ggactggcgc caacattggc aaaaacctgg ccattgtgct caacaaccgg gtgaaatcgg
     1501 cgccctcaat ccggtccgag attagtgatt cgggtgaaat taccgggaac ttcaccaagc
     1561 aatcaggtga ggacctcgct ctggtgctcc gctcaggtgc gctgccagcc cgggtcgaat
     1621 acattgaaga acgaacggtt ggcccatcgc ttggggctga ctcaatccga agcggcgttc
     1681 tggcgtcggt ggctgggttg atcttcatca tgatttttat gggaatctac tatcagtttt
     1741 cagggatcaa cgccattgtg tcattgattt tgaacctgat tatgttgatt gccgccctgg
     1801 cgatgtttag tgccaccctg acccttcctg gaattgctgg tatcattttg acgattggga
     1861 tggcggttga ctcgaatgtg ctgatctttg agcgcatccg tgaagaactc aaacttggaa
     1921 aggcggttgt ttccgcggtt gagaccgggt ttgctaaagc gttcattacc atcattgata
     1981 ctcacgtgac cacgattgtg tcctccgttt tcttgttcat ttttgggacc gggccaattc
     2041 gtgggtttgc gatcaccctc atcattgggt tgcttgccaa cctctttacc gccacctttg
     2101 tgtcgcggac gatgtttggg tatacgctgt cacgaaaaga acgtgtcacc agcctgagca
     2161 tctaaagatc aatcgggttc agggttttga ggaactccaa ctttctctaa tggttgaaac
     2221 cctgaaccct gaaccctgaa ccctgaccag accattccac cactacttca ta
//
LOCUS       DAOQDO010001006         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_43977_length_2271_cov_2.261282, whole genome shotgun sequence.
ACCESSION   DAOQDO010001006 DAOQDO010000000
VERSION     DAOQDO010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43977_length_2271_cov_2.261282"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..>2271
                     /locus_tag="PKZ53_15250"
     CDS             411..>2271
                     /locus_tag="PKZ53_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41845.1"
                     /translation="MNTHLATIEPGTTSTGPQVLVSFGMVTGLCRNDRWMFVGHPWGV
                     AIRDFKGNLVRCVALGERPRTMVADAQTLWVGTSENLFRVDLKTFGTVVVSYEDPTVQ
                     SPPVHKQDPVSQKSKQRAAFAHVKFLVIQGTRLWIGASDLFALDMGTHALRWFQPDEF
                     PARNASQWEKLFVDRDSIWVSTPYECFRYDSRYDRWEAPIEPGDTQLGLMWLIDGQLW
                     AKSVHQNQQLKCFVIDPKSRIAKPVGQDGLSLFNGLEFFGWYQGNPVFGRKVPQYVYH
                     SQTNRLLFVEKNPQLEMVELKNGIPPNLPVWGQYDRGDQVVQVGWPTETNAGQVFFSL
                     RNMLTLPDKTLVLAGGDSSHSVSSGLWLVPGQEPGPRQVFQPSLGILVTSIVPHEPTK
                     TLWVNTNEGIFVFDHQNKLVMRLGQSDGLISNFVTHGVWVGNQYICICDSAGTLFVSV
                     VDPHKTRVVTNSRLCEISNTYRIKSLTPTTSGKGVMISFELVGEYISESYRQIPDPIE
                     FPLGSGDYSEPLHLKNVPVELSSPKQLQPKLPYLGGTLGGQVQAFGKTYLYGNNGVVL
                     FQNELPVHQKQELGVRLSPAQPFWQQKEAAQMPYVHSLSPQAVLTQLDTETNPY"
BASE COUNT          584 a          521 c          577 g          589 t
ORIGIN      
        1 ggtgtcctga atgcgggacg gcgccagcgc gaacgcaact gcgtgaccga ttgtttccag
       61 tccagattgg aagtaccaga tttgagaata tttcgcaggt tgcattaacc ctgatcagac
      121 agtatgccca aagtggggac gtggaaaaat tatttgccct gatgtaccag gtcgccaacc
      181 gtaaaccgcc ttttgacagg ttcgaaaatt attggccaaa tccagtctac ttaaatcaag
      241 tgaatctggc cattggaatc gcactcaaca acctgaagaa cccggcccag gtgaaaagac
      301 tccacaccgt atatgaccag ctaccctggc ggcgttcaaa aaagcaaagt ttcttcacta
      361 ccgaggtgaa ccccaccacc ctgcccaagg attggaaatc aggttacttt ttgaatactc
      421 atcttgcgac gattgaaccg ggcacaactt ccacgggacc ccaggttctg gtttcgttcg
      481 gaatggtgac tggcctgtgt cgcaatgacc gatggatgtt tgtcggtcac ccgtgggggg
      541 ttgcgatccg tgattttaaa gggaatctgg ttcgctgcgt tgcactgggg gagagaccgc
      601 gcacgatggt tgcagacgct caaaccctct gggttgggac ttctgaaaac ctctttcgag
      661 ttgatttgaa gacatttgga acggtcgttg tttcttatga agacccaaca gttcagtcac
      721 caccggttca taaacaggat ccggtttctc aaaaatcaaa acaacgagct gcatttgctc
      781 atgtgaaatt cttagtcata caggggaccc ggttgtggat tggagcttca gacctgtttg
      841 ctctggatat ggggacccat gcccttcgct ggtttcaacc tgacgaattt ccagccagaa
      901 atgccagtca atgggagaag ctctttgttg acagagattc gatttgggtg agcacaccct
      961 atgagtgctt tcgctatgac tcacggtatg atcgctggga ggctccaatc gagcccggag
     1021 atactcaact tggcttgatg tggctcatag atggtcagtt gtgggcaaag agtgttcatc
     1081 agaatcagca actcaagtgt tttgtgattg atccgaaatc acgaatagca aaacctgttg
     1141 gccaggatgg attgagctta ttcaatggac ttgagttctt tggttggtat cagggaaacc
     1201 ccgtctttgg aaggaaggtt ccgcaatatg tataccattc acaaaccaat cgcctgcttt
     1261 ttgtggagaa gaatccccaa ctggagatgg ttgaattgaa gaatggtatt ccgccaaacc
     1321 tgccagtttg gggacagtat gatcgtggag atcaggtggt tcaggttggt tggccgacag
     1381 aaaccaacgc gggccaggta tttttttcgc tgcggaatat gctcacactt cccgataaaa
     1441 ctttggtttt ggccggaggg gactctagcc actcagtctc aagtggtttg tggttagtcc
     1501 caggccagga gccaggcccg cgccaggttt ttcagccatc cttgggaatt ttggtaactt
     1561 caatcgtgcc gcatgaacct accaaaaccc tgtgggtcaa taccaatgaa ggtatttttg
     1621 tgttcgatca ccaaaataaa ctggtgatga ggttggggca atcagacggg ttgatttcca
     1681 acttcgtaac ccacggggtg tgggttggaa atcaatacat ttgcatttgt gattcagcgg
     1741 gcaccctctt tgtttctgtg gttgacccac acaaaacaag ggttgttaca aattctcgct
     1801 tgtgcgagat aagcaatacc tatcggatca aatcacttac cccaacgaca tctggaaaag
     1861 gagtgatgat atcctttgaa ctggttggag aatatatatc agagagttac aggcagattc
     1921 ctgacccaat tgaattccct ctgggaagtg gtgattattc ggaaccgctt catttgaaga
     1981 atgtgccggt ggaactgtct tcccccaaac aactccagcc gaaattaccc tatcttgggg
     2041 ggactctcgg tggtcaagtt caggcgtttg ggaaaacata tctctatgga aacaatggag
     2101 ttgtactctt tcaaaatgag ttgccagttc accaaaaaca ggaactgggt gtcaggttgt
     2161 cgcctgctca gccgttctgg caacaaaagg aagcggcaca gatgccgtat gtgcacagct
     2221 tatccccaca agcggtgctg actcaattag atacagaaac caatccatat c
//
LOCUS       DAOQDO010001007         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44069_length_2268_cov_2.944871, whole genome shotgun sequence.
ACCESSION   DAOQDO010001007 DAOQDO010000000
VERSION     DAOQDO010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44069_length_2268_cov_2.944871"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2268)
                     /locus_tag="PKZ53_15255"
     CDS             complement(<1..>2268)
                     /locus_tag="PKZ53_15255"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ41846.1"
                     /translation="ALLADRFGQTLGKPHTVKLKFGRNEPFLYAPYSNLVILDPAGKR
                     RCDIFSQGIPALRERLFQVQPEDWPYFTQYRSTQLDEDNGPKTIAPPGKLIVDRTRPI
                     HQPKSSEQTFTEIDLNPGLVNGIGQVVLVVEPVGIPPTLEIKPEIIWFQATSLAVDVI
                     ADQDGLLVWANSLADGKPVADATVTLFPPRRSGLTDHQGLATISYSHQRETENPFVIL
                     RSGNDAVMLPGSSDWWSRAARYTPPGNREDAKCWFVVDDRELYQPGEELHFKGWIRQY
                     EPNKSGDITRAKNLGNRVAYQVFDSQQRMIANGTTQLNVFGGFDGSLILPPTVSLGRA
                     TINFRVGSTFAEIEPTEYRHYFQIQQFRRPEYELKLTASEGPHVIGTSATATISASYF
                     AGGPLKKADTRWKFSVMEGSYHPPNWDEFRFGKQSSSWWRPSGNNQTCTGSTDAQGHH
                     TVQIDFDGITRPTPVVVTAEATISDVNRQQQSASTRILVHPASLVVGLKQRQGFIFPG
                     ESFTLDSIVTDLDGTPQAGKAIRLEIVRQERRFEDGTWTQIEVDPQTTTLLSAATPVI
                     WEFSPTQSGEYQLTATVADDQNRLHQTVVSFWVAGSSQEFSSEFEEEHIKLISNRTEY
                     APGETAELLLQAPFFPAEGLLTIQRSGVIHRERFTLDQSARLFKLPIDESQIPNVTVQ
                     VNLVGEAFRRDEAGVEVTSVPKQPAYASGTVELAVSTQSRRLTVTARPVAETVAPGSR
                     TSIEIDVTDAAGKPVA"
BASE COUNT          516 a          586 c          605 g          561 t
ORIGIN      
        1 gcaactggtt tgccagcagc atcggttaca tcaatctcaa tcgaagtccg gctccccgga
       61 gccacggttt ctgccactgg tctggccgtg acggttaggc gacgcgattg ggtcgagacc
      121 gcaagttcaa ccgtaccgct ggcgtaggca ggttgtttcg gcaccgaggt cacttcgaca
      181 cccgcttcat ctcgtcggaa cgcttcgccg accagattga cctgaactgt gacatttgga
      241 atctggcttt catcaatcgg aagcttgaaa agtcgagccg actggtcgag ggtgaaccgt
      301 tcgcgatgga tgacgcctga ccgctggata gtgagcaatc cttccgctgg gaaaaacggt
      361 gcttgaagca ggagttcagc cgtttcgcct ggagcatatt ctgtgcgatt ggaaatcagt
      421 ttgatgtgtt cttcttcgaa ctcagatgaa aattcctgac ttgaacctgc aacccaaaat
      481 gaaacaaccg tttgatgaag ccggttttga tcgtcagcca ctgttgccgt gagttgatat
      541 tcaccgctct gggttggcga aaattcccag atgaccgggg tggcggctga aagaagcgtt
      601 gtggtctggg gatcaacttc aatttgtgtc caggttccgt cctcaaatcg ccgctcctga
      661 cgaacaatct ccagccgaat ggcttttcca gcctgcgggg ttccatccag atccgtcaca
      721 atgctgtcaa gggtgaatga ttcaccagga aaaatgaatc cctgccgttg cttgagtcca
      781 accacgagtg aagctggatg aaccagaatc ctggtcgaag ccgattgctg ttgccggttg
      841 acatcgctga tggtggcttc agcggtgacc acgactgggg ttggccgggt tatgccatca
      901 aaatcaatct gaacagtatg gtgaccctgg gcatcggtcg agccagtaca ggtctgattg
      961 ttccctgagg gtctccacca ggatgatgat tgctttccaa acctgaactc atcccagttg
     1021 ggcggatgat acgatccttc catcactgaa aacttccagc gggtatcagc ctttttaaga
     1081 ggacctccgg caaaatagct ggctgaaatc gttgcggtgg cggaagtccc aatcacatgc
     1141 ggtccttcgg aagccgtgag cttgagttcg tattccggac ggcgaaattg ttgaatttgg
     1201 aaataatgcc ggtactcagt cggctctatt tccgcaaagg tgctgcctac ccgaaaattt
     1261 atcgtagccc gtccaagact taccgtcgga ggcagaatca gtgacccatc aaacccacca
     1321 aatacattga gttgggttgt tccattggca atcatgcgtt gctggctgtc aaaaacctga
     1381 taggccaccc tgtttccgag gttttttgct cgggtgatat ccccgctttt gttcggttca
     1441 tactggcgaa tccagccttt gaaatggagt tcttcccccg gttgatacag ttcacgatca
     1501 tcaaccacaa accagcactt tgcatcttcg cggttgccag gcggtgtgta tctggctgct
     1561 cggctccacc agtcggaact accagggagc atcaccgcgt cgtttcccga gcgaagaatc
     1621 acaaacgggt tttctgtttc acgctgatgt gaataagaaa tcgtagccag cccctgatgg
     1681 tcagtcaggc cggaacgtcg aggcgggaac agagtcaccg tggcatcggc taccggtttg
     1741 ccatcggcca gggaattagc ccacaccagc aagccatcct gatcggcaat cacatcaacc
     1801 gccaggctgg tggcttgaaa ccagataatt tcaggcttga tttccagggt tggcgggatg
     1861 ccgaccggtt caaccaccag aaccacctgc ccaatcccgt tgaccagccc tgggtttaga
     1921 tctatttcag tgaaggtttg ttcagaagat ttcggctggt ggattggtcg ggttcgatca
     1981 acaatcagtt tgccaggagg cgcgatggtt tttggcccat tgtcttcgtc caactgggtg
     2041 gaacgatatt gggtgaaata aggccagtcc tcaggttgaa cctgaaataa acgttccctc
     2101 agggcaggga ttccctgtga aaaaatgtca catcttcgct ttccagccgg gtctaaaatc
     2161 accagattgg aatacggcgc atacaggaac ggttcattcc ggccaaattt cagtttgact
     2221 gtgtgtggtt tgcccagtgt ttgtccgaaa cgatcagcca gaagtgcc
//
LOCUS       DAOQDO010001008         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44077_length_2268_cov_2.504745, whole genome shotgun sequence.
ACCESSION   DAOQDO010001008 DAOQDO010000000
VERSION     DAOQDO010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44077_length_2268_cov_2.504745"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1474)
                     /gene="mgtE"
                     /locus_tag="PKZ53_15260"
     CDS             complement(95..1474)
                     /gene="mgtE"
                     /locus_tag="PKZ53_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNJ41847.1"
                     /translation="MDTLNQPFQLQRTVETVSRLVKRGATATYLSLLQKLRPAEVAQI
                     LINLKGRDRIEVFEQVFTTNPSLSATILSEMLAEEHGLDVLENLTAEEISDLLQHLPP
                     DDAAHLLSFLPEDRHEEVLSLMELDHSLVAQELLQFPEYTAGRIMTPDVFRLREDVTI
                     SEAIAALQSSTDRLEMVFYLYVVDDRNHLVGVVALRQLLLNPPNTPLKRLMSTDVIKV
                     HAEDDQESVARIVAQFNIVAVPVVDSNNKLIGMVTVDDVIDVMREEATEDLFALAGVE
                     SDDRAMAKPFRSLRTRFPWLVLSLVSALVPAFVVSRFQDMFSSKLALAAMLPIFATIG
                     GNAATQTLTVIVRSLSLGEVGEGTESRIFVKEVMVGLSNGLVFGLVLTILVGIWQQNL
                     QFALVVGLATICNLVVASIAGSLSPLLIRKLGVDPVLASSAIAITTTEALGFFSYLGL
                     AWLLFHWIR"
     gene            1953..>2268
                     /locus_tag="PKZ53_15265"
     CDS             1953..>2268
                     /locus_tag="PKZ53_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41848.1"
                     /translation="MGNFRLVAYLRNFLLKVSRVPREQRFYTFILAAPTATAKIHRFQ
                     LPHHLLCVVCVLAALGALTVLAGAGLDALINLSASPYHRGKLPQRVKALSAAASRVGA
                     PLL"
BASE COUNT          523 a          616 c          594 g          535 t
ORIGIN      
        1 gatgagggat gaaataaatc acccggtcca gtttctcgcc gcgtagcggt ggttgaattg
       61 aggcaggtcg tttacggcct gctgtccatc cgcatcaacg aatccagtga aaaagtagcc
      121 aggccaggcc cagatacgag aaaaatccta acgcttcggt ggttgtgatg gcaattgcac
      181 tggatgccag gaccgggtca accccaagtt ttcggatcag gagtggtgac agtgacccgg
      241 caattgacgc gacgaccaga ttgcaaatcg tcgcgagtcc aacgacgagt gcaaactgaa
      301 ggttctgttg ccagatgccg accagaatcg tcaaaaccag cccaaaaacg agtccattgc
      361 tgagtccgac catcacttcc ttgacaaaaa tccggctttc cgtgccctca ccaacttcac
      421 cgagcgacag gcttcgcaca atcaccgtca gggtttgcgt tgctgcattg cccccaatcg
      481 ttgcaaaaat cggcaacatg gcggccagag ccagctttga actgaacata tcttgaaacc
      541 ggctcacgac aaaagcgggt accagggctg agaccagact gagcaccagc cagggaaagc
      601 gggtgcgcaa cgaccgaaag ggtttggcca tggcccggtc gtcactttca accccggcca
      661 gcgcaaacag gtcttcggtg gcttcttcac gcatcacgtc aataacgtca tccaccgtca
      721 ccattccaat gagcttattg tttgaatcaa ccaccggcac ggccacgata ttaaattgtg
      781 ccacaatccg ggccaccgac tcctggtcgt cttcggcatg gactttgatg acatcggttg
      841 acatcaggcg tttgagcggc gtgttgggcg ggttaagcag taactgtcgc agtgccacca
      901 cgccaaccag atgattgcga tcatcaacca cgtaaagata aaacaccatt tccagccggt
      961 cagtgctgct ttggagagcg gcaatcgcct cggaaatggt cacatcttcg cgcaaacgaa
     1021 agacatccgg ggtcataatt cgcccggctg tatattcagg aaactgaagg agttcctgcg
     1081 cgacaagcga atgatccagc tccatcagcg acaggacttc ttcgtggcga tcttcaggca
     1141 aaaatgacag caagtgtgcc gcatcatctg gcggcaaatg ctggagcaaa tccgagattt
     1201 cttcagccgt gaggttttca agcacatcca ggccgtgttc ttcagccagc atctcactca
     1261 agatagtggc tgaaagggaa ggattggtgg tgaaaacttg ctcaaagacc tcaatccgat
     1321 cacgtccctt caggttaata agaatttggg cgacctcagc cgggcgcaat ttttggagca
     1381 ggctcaaata ggtggccgtg gcaccgcgtt tcaccagacg tgaaaccgtt tcaacggttc
     1441 gctgaagctg gaacggttgg ttgagagtgt ccataggcgt ttggggttgc gcgtttttcg
     1501 gaagggatga gggatgaggg atgaggggtg aaaaaatccc cagtcacttt gtcagtcggt
     1561 tatcttgtca gcgtttaagg ttcaaagggg tcgaatgtat gtcctttccg tccttttcgt
     1621 tctttatgtc tttttgtctt tttcctcagt gaaccccgaa ccccgaaccc cgaattggcc
     1681 cggttatatc acagccgctt tgaccctgtc aggtaagatt gctttgtccc gcaggatgtg
     1741 gctgagtacc atagacaggt tgcacacatt ggcaggttat gataccttcg gcggcctctt
     1801 aaacttcagt cggggtcttc ttctctaccc caccccaaaa tatcaattca cgcaagtctt
     1861 tcaggaatac ctggcgtttt ctccgactta tcttgccgaa gtaacggcag gtttgtgttt
     1921 ttgaggactt tgtttgaaca gagagtcact gcatgggtaa ttttcgtctg gtcgcatatc
     1981 ttcgcaattt tttgttaaaa gtctcgcgtg tcccccgcga acaacggttt tacaccttca
     2041 tcttagccgc accaaccgcc accgccaaaa ttcatcggtt ccagttgccc caccacttgc
     2101 tgtgtgtggt gtgtgtgctg gccgcgcttg gggcactcac ggtactggct ggcgcgggac
     2161 tggatgcgct gatcaacctg tccgcctcgc cctaccatcg cgggaagctg ccgcaacggg
     2221 tgaaggccct gagcgcggcc gcatcccgcg tgggtgcccc cctgctct
//
LOCUS       DAOQDO010001009         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44119_length_2267_cov_3.189873, whole genome shotgun sequence.
ACCESSION   DAOQDO010001009 DAOQDO010000000
VERSION     DAOQDO010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44119_length_2267_cov_3.189873"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKZ53_15270"
     CDS             complement(<1..183)
                     /locus_tag="PKZ53_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41849.1"
                     /translation="MSRALACAPASTVPAFSNQLLNRWLQDSFARFESPSLSQAQGPA
                     VIVYETNEAFVVQAELP"
     gene            complement(441..1952)
                     /locus_tag="PKZ53_15275"
     CDS             complement(441..1952)
                     /locus_tag="PKZ53_15275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41850.1"
                     /translation="MGLFDQIRSITHQMSTMLDDAKARREIDHYEQELRTNPNSVHAL
                     QELSALYQEIGENNKAAEILARAADLHRQRQSIDLALGLLRQAERLATKEQRPAIIRP
                     LVEIYTQLKRYDEAAQHIRQALDYFITSQKQAEAVAFIESLPPLGKKDQLYRGEFMEL
                     MRVGPEGKSVESSGSWVIDKATLPGGGMFPKISDKFPEQTLLIADDDPEMLQVLTTLF
                     VPFACKIITATNGEEALAKAIEHKPTLIVSDLIMPRMDGCEFFTALRQHPTLSEVPFV
                     CMSSRGQEEERLAALAQGVEEYWVKPFVLPELLFKTRNLLKRLRPPADLSGRLSEMSV
                     VELLQFLDSKKNTGILTLRSKPHKAWVYISDGRVINATMDSIQGRMVIYKTVYWLTGD
                     FEFRSCQVNPTDTIELPTQTLVVEAIRRYQQAQQIIDTLPDQSLVFKSTIELQQLTAT
                     EPELAAHFNYLIAVFNGQRSLQECLSILNGDLETLVLAQSLIKQNYLIPLSAG"
     gene            complement(1876..>2267)
                     /locus_tag="PKZ53_15280"
     CDS             complement(1876..>2267)
                     /locus_tag="PKZ53_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41851.1"
                     /translation="RNPEPGTRNPEPGTRNPQSPPESSGNLLFGTCKKTVARKPKAVH
                     AYDFRPFLSVTFKTLPRGQPAGFSKPATPGSVSERLTGVGFSLATVDDKPGKLTVHQG
                     RSYGTVRSNSLNHTPDVHDAGRRQSPA"
BASE COUNT          512 a          527 c          632 g          596 t
ORIGIN      
        1 gggcaattca gcctggacga caaacgcttc gttggtttca tacacaatca cggctggacc
       61 ctgggcctgg ctcagggatg gcgattcaaa tcgggcaaat gaatcctgca accagcgatt
      121 gagcaattga tttgaaaaag ctggtacggt tgaagctggg gcgcatgcaa gtgctcggga
      181 catattttga gaaacctcct ggtgttcaat aattgaactg atgtcatctg tgtaaaagtg
      241 tttgtgttga tgttgttgct ataataaaaa tctgagtgta ttgatgtcaa gtatgtttta
      301 ccggtaatca ctaagctttt attgagtaat atagaatgat agttttctga ctgttttact
      361 ggagataaaa aaccccagcc tgtgtctgca atcagactca ggctggggca agcgactcgc
      421 cgcgtatttt ttaggagtag ttacccggca gataagggaa tcagatagtt ctgcttgatg
      481 agggattggg ccaggaccag ggtttccaga tctccgttca gaattgacag gcattcctgc
      541 agcgagcgct gaccattaaa cacggcgatt aaataattga aatgtgctgc cagttccggt
      601 tcggtggcgg tgagttgttg gagttcgatg gtggatttaa agaccagaga ctgatcaggc
      661 agggtgtcga taatttgctg ggcctgttga taccggcgaa tggcttcgac caccagggtt
      721 tgggtgggca attcaatcgt gtcggtgggg ttaacctgac aggaccggaa ctcaaaatcg
      781 cctgtcaacc agtacaccgt tttgtaaatg accatccgcc cctgaattga gtccattgtg
      841 gcattgataa cccggccatc gctgatatac acccaggctt tgtgtggttt ggaccgaagg
      901 gtcaaaatcc cggtattctt ttttgaatca agaaattgca ggagttcaac cacgctcatt
      961 tccgaaagcc ttcctgacaa atcggcgggt gggcgcaatc gctttaacag atttcgtgtt
     1021 ttaaagagca actcgggcag gacaaacggt ttgacccagt attcttcgac tccctgagcg
     1081 agcgccgcga gtcgttcttc ttcctgcccg cgtgaggaca tacagacaaa aggaacttcg
     1141 ctcagggtcg ggtgctgtcg gagggccgta aagaactcgc acccgtccat gcgcggcata
     1201 atcaaatctg aaacaatcaa tgtgggtttg tgttcaatgg ctttggccag tgcttcttcg
     1261 ccgttggtgg cggtgataat tttgcaggca aacgggacaa acagcgtcgt cagcacctgg
     1321 agcatttctg gatcatcgtc agcaatcagc agggtttgct cgggaaattt gtcgctgatt
     1381 ttgggaaaca tccccccacc aggtaacgtc gctttatcaa tcacccagga gccggatgat
     1441 tcaacggatt tgccttccgg cccaacccgc atcagttcca taaactcgcc ccggtacaac
     1501 tgatcttttt tcccaagtgg cggcaggctt tcaataaagg ccactgcttc agcctgtttt
     1561 tgactggtga tgaagtaatc cagcgcctgt cggatatgct gggctgcttc atcatagcgt
     1621 ttcaactggg tatagatttc aaccagtggg cggataatgg ctggacgttg ttctttcgtc
     1681 gccagccgct ctgcctggcg cagcaggccg agcgccagat caatggattg gcgctggcga
     1741 tgaaggtctg ccgcccgggc caggatttca gccgctttgt tattttcacc gatttcctga
     1801 tacaacgcgc tgagttcctg cagcgcgtga acactgttcg gattggtgcg gagttcttgt
     1861 tcgtagtggt caatttcacg ccgggctttg gcgtcgtcca gcatcgtgga catctggtgt
     1921 gtgattgagc gaatttgatc gaacagtccc ataggatctt ccttggtgaa cggtgagttt
     1981 ccctggcttg tcgtctacgg ttgcaagcga aaacccaaca cctgtcagac gctcactgac
     2041 tgagccaggt gtggctggtt tcgaaaaccc cgctggttgc ccccttggaa gtgtcttgaa
     2101 agtaacagaa aggaaaggac ggaagtcgta agcatgcacg gctttgggtt tacgggcaac
     2161 tgtctttttg caggtgccaa aaagaaggtt gcccgaagat tccggcggcg attgagggtt
     2221 ccgggttccg ggttccgggt tccgggttcc gggttccggg ttccggg
//
LOCUS       DAOQDO010001010         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44167_length_2266_cov_2.554048, whole genome shotgun sequence.
ACCESSION   DAOQDO010001010 DAOQDO010000000
VERSION     DAOQDO010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44167_length_2266_cov_2.554048"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKZ53_15285"
     CDS             <1..1140
                     /locus_tag="PKZ53_15285"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNJ41852.1"
                     /translation="WYYFRYCDPKNDQLPFDPAIVKYWMPVDFYIGGIEHAVLHLIYT
                     RVWTMIMRDLGLVEFGEPVKTLLTQGMVILNGAKMSKNKGNVVDPDEMVDRYGADATR
                     LSILFAAPPEKEVDWKQSLRVIAPEHPLFDQVLKGTRAFDDQNRLSFILEQPDESTLT
                     LPDQDVSVEYPAAEGAMRYLARVWRLVRKWHALAATTMFDSEPTAYSDAQQTIRRATH
                     QTIQRITHAYEEGLRLNVTVAACMELTNALYDFDATVPEVSQAGLADIFVIKEGLEAL
                     VVMLSPLAPHISEELWEALGHTSSVSKARWPVFVAEWAREAQLEIPVQINGKLRTKVL
                     VDAEATEEQMKATVLADPKVQSSLEGKTIVKTIIVPKRLLNLVVK"
     assembly_gap    1352..1451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1586..>2266)
                     /locus_tag="PKZ53_15290"
     CDS             complement(1586..>2266)
                     /locus_tag="PKZ53_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41853.1"
                     /translation="LLEKLGYRADVAVNGRDACLRVEQKPYDLLLMDIQMPEMDGLEA
                     TRWIRGRPQMRQPWIVAMTANAFDQDREACFEAGMNGVVVKPVRIEELEAMLVQVPRH
                     WGSDQRSASGMVLQTEVLERLAAIFDGQFTEILSHRLKELRGHLQGMGQALECGDLDR
                     LHREVPLIKSKGGILGAERLSALCEAVRPDQSLVVQRELWQQLGQELGALEQEIETWI
                     RGRAEKTG"
BASE COUNT          471 a          684 c          510 g          501 t
ORIGIN      
        1 tggtattatt tccggtactg cgatccaaaa aacgatcaac tcccctttga tcccgcgatt
       61 gtcaaatact ggatgccggt tgatttctac atcggcggca tcgaacacgc cgtcctgcac
      121 ctgatttata cccgggtctg gaccatgatt atgcgcgatt tgggtctggt tgaatttggc
      181 gaaccggtca aaaccctgct cactcaggga atggttatcc tcaacggtgc caaaatgtcg
      241 aaaaataagg gcaatgtggt tgatccagac gaaatggttg accgctatgg cgccgatgcc
      301 acccggctct cgatcctctt tgccgcacca ccggaaaaag aagttgattg gaaacaatcc
      361 ctccgggtca ttgccccaga acatccgctg tttgatcagg tgttgaaggg aacccgtgcc
      421 tttgatgacc agaaccggct ctcgtttatt ttggaacaac ctgatgaatc aaccctgacg
      481 ttgccggatc aggatgtatc ggtcgaatac ccggctgcgg aaggtgccat gcggtatctg
      541 gcgcgtgtct ggcgactggt tcgtaagtgg catgccctgg ccgcaaccac catgtttgac
      601 agtgaaccca cggcctattc tgatgcccag caaaccattc gccgggcaac ccaccagacc
      661 attcagcgca ttacccatgc gtatgaggaa ggcctccgac tcaatgtcac ggttgccgcc
      721 tgtatggaac tgaccaatgc cctgtatgac tttgatgcca ccgtgcccga ggtttcacaa
      781 gcgggcctgg ctgatatttt tgttatcaaa gaagggcttg aagcgcttgt ggttatgctg
      841 tcccctctgg caccgcatat ttccgaagaa ctctgggaag cactcggcca cacatcctcg
      901 gtctcaaaag ccagatggcc ggtctttgtg gccgaatggg cacgcgaagc tcagcttgag
      961 atcccggttc aaatcaatgg aaagctgcgc accaaagtcc tggttgatgc cgaagccacc
     1021 gaagaacaga tgaaagccac ggttctggcc gaccccaagg ttcaatcctc cctggaaggc
     1081 aaaaccatcg tcaaaaccat catcgtgcca aaacgactgt tgaatctggt ggtgaaatag
     1141 ggcgaaccac cgccggggca actcaagggc tgggggcttg gggctgaggg cttggggtat
     1201 taaaacccgt cttcttcagc ccgttttttt cagccctcag ccctcagccc tcagcccatt
     1261 ttttcaaccc tcagcccgtt ttttcagccc gacctcttca gcccgacctc ttcagcccga
     1321 cctcttcagc ccgacctctt cagcccgacc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn ncttcagccc gaagtcttca gcccgacctc ttcagcccgt cttcttcagc
     1501 cctcagcccg tttttttcag ccctcagccc gttttttcag ccctcagccc gtttttttca
     1561 gccctcagcc cgtttttttc agccctcagc ccgttttttc agcccgacct cgaatccagg
     1621 tctcaatttc ctgctccaga gcaccaagtt cctgcccaag ttgctgccag agttcacgct
     1681 gaacgaccag actctggtct ggtctcaccg cctcacagag tgccgaaagt cgttcagctc
     1741 ccaaaatccc ccccttgctt ttgatgaggg gaacctcacg atgcagcctg tcaaggtcac
     1801 cacactcaag cgcctgaccc ataccctgga gatgcccccg gagttccttc agccggtgac
     1861 tgagaatctc tgtaaactga ccatcaaaaa tggcagcaag ccgttctaaa acctcggttt
     1921 gaaggaccat gcccgaagcg ctcctctggt cagatcccca gtggcgcggc acctggacca
     1981 gcattgcctc caattcctcg atccgaactg gcttgaccac caccccattc ataccagcct
     2041 caaagcaggc ttcccgatcc tggtcaaagg catttgccgt catggccaca atccagggct
     2101 gccgcatctg tggtcggccc cgaatccagc gagtcgcttc cagtccgtcc atttccggca
     2161 tctggatatc catcagaagc aaatcatacg gtttctgctc aacccgaagg caggcgtcac
     2221 gcccgttgac cgccacatcg gcccggtacc caagtttttc aagaag
//
LOCUS       DAOQDO010001011         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44186_length_2265_cov_3.726697, whole genome shotgun sequence.
ACCESSION   DAOQDO010001011 DAOQDO010000000
VERSION     DAOQDO010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44186_length_2265_cov_3.726697"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1977)
                     /locus_tag="PKZ53_15295"
     CDS             complement(196..1977)
                     /locus_tag="PKZ53_15295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ41854.1"
                     /translation="MSSYQGDEKVERMLYSLTALADIGEAITAGSSFETTIRELLHLI
                     LGTLTVSKGAILLHTPETSTLRLLVGRGVDTACPVIPFPAEMVKAFCENQQPVIFSQA
                     DHVFASFVDRNHEALTKLHAYVWTPLIVGNRLLGILNLSEKFGKHPYTDDDLQMLNTI
                     AHHTSIAIYNYQIIDELRATNFKLNRKVLEMESLYDVGLAISSLLDLAELSREILQRA
                     VMLLDASRGVLFFTEGTSLLRVAASFGMEDRYPEGALVTLHQVPPQLVEVAITGKCSC
                     ILNERSRSLAAEAQNLLIVPILIAQRVIGVLMVSDKETREGFAEFTDDDEQFLAAICN
                     QAAIAIDNARLHKEALEKERIEKELEVAATIQQRLLPDAAPSLDGIELVGVNMSCRQV
                     GGDYYDYIQFDNHQLGIAIADVSGKGTPAALLVSTLQASFHALLQNYDLTETVTRLNK
                     AIFKASPSYNYITFFYGVIDLRTLSFCSINAGHNDTFLIDASGEVRRLSTGGFCLGMF
                     DFATYEVEQTFLKPGDIIFLYTDGVTESNNLAGDEFGEEQALELLIANRHLPMAELSN
                     LLSEAIKEFVGEAPQHDDLTFVLVKVG"
     gene            complement(2073..>2265)
                     /locus_tag="PKZ53_15300"
     CDS             complement(2073..>2265)
                     /locus_tag="PKZ53_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ41855.1"
                     /translation="LQKTQLVNSIGISILIEVIEKVQEIGGTLAFCHLTPIIAKTFKI
                     MGLTQYAKVYDTEEDALKA"
BASE COUNT          632 a          538 c          536 g          559 t
ORIGIN      
        1 gaagaatgaa gaatgaagaa ttgagaatga agaattgaga atgaagaatg aagaatgaag
       61 aatgcggtta gtatttggtt cttagtcctt ggaaatcaat gctttccaag aaccaagaac
      121 caagaaccaa gaaccaagaa ccaagaacca ggcaccaaga accaggcaca ttcttcattc
      181 ccaattcaaa aagggttagc cgactttaac cagcacaaag gtcaggtcat cgtgttgtgg
      241 tgcttctccc acaaattcct tgattgcttc agataataag tttgaaagtt cagccatcgg
      301 caggtgccgg ttggcaatca agagttcaag ggcctgttcc tcaccaaatt catctccggc
      361 cagattattt gattcagtca ctccatcagt atagagaaag ataatatcac cgggtttcag
      421 gaacgtttgt tcaacctcat aggtggcaaa atcaaacatc ccaaggcaaa acccaccggt
      481 tgaaagtcgt cgaacttcac ctgacgcatc aattaggaag gtgtcattgt gaccggcatt
      541 gattgaacaa aacgacaggg tcctcaggtc aatcacgcca tagaaaaagg tgatgtagtt
      601 gtacgaagga gacgccttaa aaatggcctt gttcaaccgg gtgacggttt ccgtgaggtc
      661 ataattttgc agtaaggcat gaaaacttgc ctgaagggtt gagaccagca aggcagccgg
      721 cgtccctttg cccgaaacat cggcaatcgc aatgcctaac tggtgattgt caaattgaat
      781 ataatcgtaa tagtcacccc caacctgccg gcacgacata ttgacaccaa ccagctcaat
      841 gccatccagg ctgggtgctg catctgggag caatcgctgt tgaatggttg cggctacttc
      901 cagttccttt tcgatacgct ccttttcaag ggcttcttta tggagtcgcg cattatcaat
      961 ggcaatcgct gcctgattac aaattgctgc cagaaactgt tcgtcatcgt cggtaaattc
     1021 agcaaatcct tcacgggttt ctttgtctga aaccatgagc accccgatga cgcgttgggc
     1081 gatcaaaatg gggacaatca gcagattttg ggcttcagcg gcgaggctgc gacttcgttc
     1141 atttaaaata caactacatt tgcccgtgat ggcgacttcg accagttgtg gtggaacctg
     1201 atgcagggtg accagtgcgc cttctggata acgatcttcc attccaaaag aagctgccac
     1261 gcggagcagg ctcgtgcctt cagtaaaaaa caacacgccc cgactggcat caagcagcat
     1321 caccgcccgc tgcagaattt cacgggaaag ctcagccaga tccagcagcg agctaatggc
     1381 cagccccaca tcatagagtg attccatttc aagcactttt cgattgagct tgaagtttgt
     1441 cgcccgaagt tcatcaatga tctgatagtt atagatggca atcgaagtgt gatgcgcaat
     1501 ggtattgagc atctggagat catcatcggt ataggggtgt ttcccgaatt tctcgctcag
     1561 attcaaaata cccaataacc gattgccaac aatgagtggt gtccagacat aggcatgcag
     1621 tttggtcaac gcctcgtgat tgcgatcaac aaaagaagca aacacgtgat cagcctgtga
     1681 aaaaatcacg ggttgttgat tttcacaaaa agctttgacc atttctgccg gaaaaggaat
     1741 gaccgggcag gctgtatcaa ccccccggcc aaccagcagt cggagcgttg atgtctctgg
     1801 cgtatggagc aaaatcgcac ctttggaaac cgttaacgtg cccaggatca aatgcaaaag
     1861 ctcgcggatt gtggtttcaa agctgcttcc tgccgtgatg gcttcaccaa tgtcagccag
     1921 tgcggtcagg ctatagagca ttcgttcaac tttttcatcc ccttgatagc ttgacattca
     1981 gtcctcatcc tttagaaggg ttataaaccg gggaagtgcc tggtttgcca accagttcaa
     2041 ccactaaaaa gggctcagcc catcaggtga actcaggctt tgagcgcatc ttcttcggtg
     2101 tcatacactt tggcgtactg ggtcaagccc ataattttaa atgtcttggc aataatcgga
     2161 gtcagatgac agaaagcgag tgtcccgcca atttcctgaa ctttttcgat gacctcgatc
     2221 aggatggaaa ttccgatgct gttgacaagc tgggtttttt gcagg
//
LOCUS       DAOQDO010001012         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44287_length_2263_cov_2.717844, whole genome shotgun sequence.
ACCESSION   DAOQDO010001012 DAOQDO010000000
VERSION     DAOQDO010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44287_length_2263_cov_2.717844"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKZ53_15305"
     CDS             complement(<1..377)
                     /locus_tag="PKZ53_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41856.1"
                     /translation="MASELRFLFPTTASDLQVVRAVLDGKVEAFGVLVERYQSRVFNL
                     VNRMVGNRADAEDLSQEIFCKLYQNLHRYDQSQPLQNWLLRIASNHTINFLNQRRLPT
                     TPIDREDSQGEIHPLQLPDHAPTP"
     gene            659..2053
                     /locus_tag="PKZ53_15310"
     CDS             659..2053
                     /locus_tag="PKZ53_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ41857.1"
                     /translation="MENQDQSAHLRRELGFRDVFFFYVITVFNLRWLATAAAAGPSAI
                     TIWVIACLALFVPLVFCVLELSSRYPEEGGLYVWSKRAFGNYAGFMTGWNYWASNLPY
                     FPGLLYFVAANILFVGGDSLQHLSTSPVYFISVALIGLTLAVVLNVIGLDVTKYLYAA
                     GAVCLWTATGIVMVLGGLAWWKFGSANEISFRTLIPSTNLKDVVFWSTIAFAFGGVES
                     ASTMGDEIRDARRTIPRAIIAAAVVITGLYILGTLSVLLALPQSEVSSLQGILQAVSK
                     VEQRIGVSGLSELVALLVAISVLGGIGAWFAATARLAFVAGLDKFLPPAFGKIHPKWG
                     TPYVALLVQAGVAAVFAFLGQAGTTVRGAYEVLVSMGIIANFIPFLFMFAALIKVQSF
                     PAGPDVLRVPGGKPVAIIAGALGFFTTALSIVLATIPSESEPNPTLAVVKIIGLTFVL
                     LLVGSGIFVAGNKK"
BASE COUNT          503 a          501 c          623 g          636 t
ORIGIN      
        1 ggtgttgggg catgatcagg cagttggagc gggtgaatct caccctggga atcttcgcgg
       61 tcaatcggcg tggttggtaa ccgacgttga ttgaggaagt taatcgtgtg attgctggca
      121 atccgtaaga gccagttctg aagcggttgg ctttgatcat agcgatgcaa attttgatag
      181 agcttgcaga aaatctcctg cgagagatct tcggcatcgg cgcgattgcc aaccattcgg
      241 ttgacgagat taaacacccg cgattgatac cgctcgacga ggaccccgaa ggcttcaacc
      301 tttccgtcca gcacggctcg aacgacctga aggtcgctgg ccgtggttgg aaaaaggaaa
      361 cggagttcgg aagccataca gaatgaagaa tgaagaataa agaatgaaga attgagaatg
      421 aagaatgaag aaaatatgtt aaaaagtcac caggaaaaaa ctgtaaccca aggttagtca
      481 tcagattaca ggcagtcacc caggtcttag cgggagagcc aaatacgtcg aaagacctca
      541 taactcaaat tgataaagta gtttcatggc attctcaatt ctcaattctt cattcttcat
      601 tcttcattct gttctatagt gaagtaccac aaacctcacc tctgggattc agcaaaaaat
      661 ggaaaatcag gaccagtcag cgcacctgcg ccgggaactc ggctttcgcg atgtgttctt
      721 tttctatgtc atcacggtat ttaacctgcg ttggctggcg acggcggcag cggcggggcc
      781 gagtgccatc acgatctggg tcattgcctg tctggcgtta tttgtgccgc tggtgttctg
      841 tgtgttggag ttgtcatcac ggtacccgga agagggcggc ctgtatgtct ggagcaaacg
      901 tgcctttggg aattatgcag gttttatgac cggatggaat tactgggcgt caaatctgcc
      961 ctattttcca ggactgctgt attttgttgc cgccaacatt ttatttgtcg gtggagattc
     1021 gctccaacat ctgtcaacca gccccgtcta ttttatttca gttgcactga taggtttgac
     1081 gctggcggtt gtgttgaatg tgattggact tgatgtcacc aaatatctct atgccgccgg
     1141 tgcggtgtgc ctgtggacgg cgactggaat cgtgatggtg cttggtggac tggcatggtg
     1201 gaaatttggt tccgccaatg aaatcagctt tcggactctg attccgagca ccaatctcaa
     1261 ggatgtggtt ttctggtcca cgattgcctt tgcctttggc ggtgtggaga gtgcttcgac
     1321 catgggtgat gaaatccgcg atgcccggcg gaccatccct cgggccatta tcgcggcggc
     1381 agtggtcatt accgggttgt atatcctggg gacattgagt gtcttactgg cgttgccgca
     1441 atcagaagtg agcagcttgc aaggcatttt gcaggcggtt tccaaagttg aacaacgaat
     1501 tggcgtttcc ggactttcag aactggtcgc cttgctggtt gccattagcg tgctgggcgg
     1561 tatcggcgcc tggtttgccg ccaccgcccg actggccttt gtcgccggat tggacaaatt
     1621 cctgccgccg gcgtttggga aaatccaccc gaaatgggga accccctatg tggccttact
     1681 tgtgcaggcc ggagttgcgg cggtatttgc ttttttaggg caggctggaa ccaccgttcg
     1741 cggagcgtat gaagtgctgg tcagtatggg gattatagcg aacttcatcc cgtttctgtt
     1801 tatgtttgcg gcgttaatta aggttcaatc cttcccggct ggcccggacg tgttgcgcgt
     1861 gccgggtgga aaaccggtgg ccattatcgc cggagccctt gggtttttca ccacggcgct
     1921 ctcaattgtc ctggcgacaa ttccttcgga aagcgaaccc aaccccacgt tggcggtggt
     1981 caagattatt gggctgactt ttgttctctt gctggttggg tcagggattt ttgtagcggg
     2041 gaacaaaaaa tgataaatgt tgaggtaatt ttacttttca atgctctgtg tgttagcggt
     2101 gttgggaaag caatcactgc tcaaaattgt attgattcta aaactttaat ttgaattcga
     2161 aaatctcttt atgagtaatt attttagccc tgtttttcca caaatgggta atatgacctt
     2221 tggctaattg ccttcttttg ccgagtttac agaaattctt gat
//
LOCUS       DAOQDO010001013         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44457_length_2258_cov_4.109396, whole genome shotgun sequence.
ACCESSION   DAOQDO010001013 DAOQDO010000000
VERSION     DAOQDO010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44457_length_2258_cov_4.109396"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..2073
                     /locus_tag="PKZ53_15315"
     CDS             73..2073
                     /locus_tag="PKZ53_15315"
                     /inference="COORDINATES: protein motif:HMM:NF025697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HNJ41858.1"
                     /translation="MKTRKTSLFAVLFLVLSAFNFSPSVTKPFETVVKAQSATDPAEI
                     IFATTSTNSLISFSTSAPTSLLSNVAISGLADGERFVGIDFRPATGQLYGVTTASRMY
                     IINPGTGAASAVGTAAFTPAANGGNFGFDFNPVPDRIRFVSDSEQNLRLNPNTGGIAG
                     TDTNLAFAQGDPNAGANPSISGVAYTNNFAGTPQTTLYGIDTGLDVLVRQGDFTGAPI
                     SPNSGQLFTAGPLGVNATGRVGFDIANADGRGFATLNVDGDPGSSLYQINLINGRASF
                     FGFVGNQVVVESMSIAPHPRVFGLTENNALVTFDGTNPGPVLSRVQIFGTQAGEAILG
                     IDFRPATGVLYALGSSNRVYTIDTRTGEAKAVGTAAFTPALAGTSFGFDFNPVPDRIR
                     VTSNTGQNLRLNPDTGAVAATDTALAFAATDRNAGVTPSVVGSAYTNSVNLATTTTLY
                     NIDFNLDSLLTQNPPNDGVLNTIGRLGVNATSAVGFDISSIDGSALASITPEGAAFTN
                     LYRVSLVSGQATLVGRIGDRIRDVAIDLNQARLAVNQSADKTSIKAGETLTLTITVNN
                     FGPDVATNVTVRNPFSATLVSVDSATANQGTATTATADNVITVTGNLGSIPAGGSATV
                     TIMLKANAGATGQINNTVTVLTGSGDINRGSNTSVLTLNITQ"
BASE COUNT          496 a          647 c          549 g          566 t
ORIGIN      
        1 tgaatcaccc ccttgcttcg gacgaagcgt ctgaactgtt gttaactgta ccttttcaag
       61 gaggatccct ttgtgaaaac ccgcaaaaca tcactatttg ccgtgctgtt tctggtcctg
      121 tctgcgttca attttagtcc ctctgtcaca aaaccatttg aaactgttgt caaagctcaa
      181 tcagcaactg atccagcaga aatcattttt gcaaccacat ctaccaattc gttaatcagt
      241 ttttcaacct cagcaccgac ctcactttta tccaatgttg caatttcggg attagccgac
      301 ggtgagcgtt tcgttggtat tgatttccgc ccagccaccg gtcagcttta cggtgtcacg
      361 acggccagcc ggatgtatat tatcaaccct ggtactggcg cggcctcagc cgtaggaacg
      421 gctgctttta ccccggcggc caatggcggt aactttggat ttgactttaa cccggttcca
      481 gaccggattc gttttgtctc ggactcagaa caaaacctcc gtctcaaccc aaataccggc
      541 ggaattgccg gcaccgacac caatctggcg tttgctcagg gcgatccaaa tgccggtgcc
      601 aacccgtcaa tttcgggtgt ggcgtatacc aacaactttg ctggaacgcc tcaaaccaca
      661 ctgtatggca ttgacactgg attggatgtt ctggtccgac agggtgattt tacgggtgct
      721 ccaatttccc cgaactccgg tcagttgttc acggctggcc cactcggtgt gaatgcaacc
      781 ggtcgggtcg ggtttgacat cgccaatgct gatggtcgcg ggtttgccac attgaatgtg
      841 gatggtgacc caggctccag cctctatcag atcaacctga tcaacgggcg tgcctcattt
      901 tttggctttg ttggaaacca ggttgtggtt gaatcaatgt caattgctcc acacccacgc
      961 gtttttggtt tgaccgaaaa taatgcgctg gtgacctttg acggcaccaa tccgggccca
     1021 gtcctgagcc gcgttcagat ctttggcacc caggcgggtg aggctatcct gggtattgat
     1081 ttccgcccag ccaccggagt gctgtatgcc cttggcagct ccaaccgggt ttacacaatt
     1141 gatacccgaa cgggcgaagc caaagccgtt ggaaccgccg cctttacgcc agcgctggcc
     1201 ggcacctctt tcgggtttga ctttaacccg gttcctgacc ggatccgggt aacctccaat
     1261 actggccaga atctgcggct gaacccagat actggtgctg tggcggcaac tgataccgcg
     1321 ctggcctttg ccgcgactga tcgaaatgcc ggagtgaccc cctccgtggt tggttcggcc
     1381 tataccaaca gcgtcaacct ggccaccacc accacgttgt ataacattga ttttaacctg
     1441 gacagcctct tgacccagaa tccgccaaat gatggcgtct tgaacacaat tggacggctc
     1501 ggtgtcaatg ccacgtcagc ggttggtttt gatatttcct caattgacgg ttcagctctg
     1561 gcctcaatta cacccgaagg cgcagccttt accaacctgt accgcgtcag tctggtctct
     1621 ggtcaggcaa cgcttgttgg tcggattggt gaccggatcc gtgatgtcgc gattgatctg
     1681 aatcaggctc gtctggccgt caaccagtct gccgataaga cttcgatcaa agcgggtgaa
     1741 acgctgacct tgaccattac agtcaataac ttcggaccag atgttgccac caacgtcacc
     1801 gtccgcaatc cgttctcagc cacgttggtt tcggttgact cagccactgc caatcaggga
     1861 acggcaacca cggcgacggc tgacaacgtc atcaccgtga ctggcaacct gggctcaatt
     1921 ccagccggtg gttctgccac cgtcaccatt atgttaaagg ctaatgccgg cgccacgggt
     1981 caaatcaaca ataccgtgac ggtgttgact ggcagcggcg atatcaaccg tggcagcaac
     2041 acatcggtcc tgaccctgaa catcactcaa taacagcctt cccgcaagat ggtggcattg
     2101 agtgttaaac caaaaatcct ctctgtttcc aaagggcaat cccgtctggg attgcccttt
     2161 ttttgtcggt gaaaacaatt ccggctcacc ggaataaaca ctggccggca ctgatttccg
     2221 aaaccttcca gtttaatttc aatctgttca aatggagc
//
LOCUS       DAOQDO010001014         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44522_length_2256_cov_3.228532, whole genome shotgun sequence.
ACCESSION   DAOQDO010001014 DAOQDO010000000
VERSION     DAOQDO010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44522_length_2256_cov_3.228532"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>2256
                     /locus_tag="PKZ53_15320"
     CDS             158..>2256
                     /locus_tag="PKZ53_15320"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ41859.1"
                     /translation="MYLSFYSLREWPFALSSDTRFFYESSAQAEIRRQLREGIFARQG
                     LLTLTGPAGSGKTFLLQVLREELKSQILFGHIPDPRSIGSNLLEFLADQFGLIPDPEA
                     RLSLPTRFHRLLQDRHHQGQVTTLVVDRADEMDQSLLSSLCLLAHHEENGVRLLQIVL
                     SGRSDLERRLKRLPGYSPAAHRVRVALSPLTADEAVQFVQTRLRRAGAKRSPFTSSAI
                     QALSVLSDGNPGSLCRLCDGALLAGYEAGLTEISDSDINHTALEFGLAEDPGSLERKP
                     VTTSVSTPPRPVTQLIEKLAEPLPTADPPAIDPSSASALEKRPAAPLTDNRSTATGLE
                     SVSGQSTQLEKLSTASLVVTERPVGRENRWMAALLLFLVAVTSSVYFLRQYFNGPPIP
                     TSPAARATSLSNTLFTGKLYPSQEWKAVAPQASVVRNLSVKIGDQVKAGQVLLILDEA
                     EARRTVELARIERDLARQERDQLALEVKSGLPSKTDVATAAGEVAVAQRRAESVPVPQ
                     RQDSVDRARIVFEQARIRLERYERLYHEGLVSRDKYDEYAAAYSLAETDLQAAEAAAA
                     AQKRLVSAQQKQTDKTSKVAAQEDAADQRRRLGALETATQRLERTEAELKLAEQRLTD
                     CTVRATVDGHVIAIPVGIGDQVASGNTLMTFARLDTLVVRVPVASPFIDALHVGDPAQ
                     VILPTLPPETVPGKVVTL"
BASE COUNT          494 a          672 c          572 g          518 t
ORIGIN      
        1 attctcaatt cttcattctt cattcttcat tctcaattct tcattcttca ttctgccggt
       61 tggtttctgc tacactggtg acaattcaga gtcagtgttt ttcaaatttt ccaggtttta
      121 tttacttttt gattctaccg aagtttttca gaagactatg tatctttctt tctacagtct
      181 tcgcgaatgg ccgtttgcac tttcatccga tacccggttt ttctacgaat cttccgctca
      241 ggccgagatc cgcagacagt tacgggaagg gatttttgcc cgtcaggggc ttttgacctt
      301 aacaggtcca gccggaagtg gaaaaacctt tttgctccag gtcctgcgcg aagaactcaa
      361 atcacaaatc ctgtttggcc acattccgga tccacgctca atcggttcta acttgcttga
      421 gtttctggcc gatcaatttg gattgattcc tgacccggag gcccgccttt cgcttccaac
      481 tcgctttcat cgcctgctcc aggaccgcca tcatcaggga caggtgacaa cgctggtggt
      541 tgatcgggct gacgaaatgg atcaatcact tctttcgtca ctgtgtctgc tggcccacca
      601 tgaagaaaat ggggttcgac tcctccagat tgtcctgagt ggccgatctg atttagaacg
      661 ccggctcaaa cgcctgcctg gatattcacc cgccgcccat cgagtccggg ttgcgctgtc
      721 cccgttaact gccgatgaag cagttcaatt tgtgcaaacc cgtctccggc gagctggcgc
      781 caaacgctca ccttttacat cgagcgccat tcaagcgctg tcggtgttaa gtgacggcaa
      841 tcctggcagt ttatgccggc tctgcgacgg cgcgctcctg gctggctacg aagccgggct
      901 gacggaaata tctgattcag atattaacca cacggcactg gaatttggtc tggccgaaga
      961 tccaggttcg ctggaacgca agccagtcac cacctcggtc agtaccccac cgcgaccggt
     1021 cacccaactg attgaaaaac tggcggagcc gctccccacg gctgacccgc ccgccataga
     1081 cccctcatcg gcctcggcac ttgaaaaaag acccgcggca ccgctcactg acaatcggtc
     1141 tacagctacg ggattagaat ctgtttctgg acaatcaacc cagcttgaga aactttcaac
     1201 ggcgtcactg gtggtgaccg aacgcccagt cggtcgggaa aaccgctgga tggcagcact
     1261 gctcctgttc ctggtggccg tgacctccag tgtgtatttt ctgcgccagt atttcaatgg
     1321 accgcccatt ccaacctcac cagccgcccg ggcaacctca ctgtccaaca cactctttac
     1381 cggaaaactc tatccatccc aggaatggaa agccgtggca ccccaggcaa gcgtggtccg
     1441 caatctgtcg gtcaaaattg gcgaccaggt caaagcggga caggtacttt tgatcctgga
     1501 tgaagccgaa gcccgtcgga cagtcgaact ggcccgaatt gaacgcgatc tggctcgcca
     1561 ggaacgcgac cagcttgcgc tcgaagtcaa atccggcctg cccagcaaaa ccgatgtggc
     1621 cacagcggct ggcgaagtgg cggtggccca acgccgggca gaaagtgtcc cggtaccgca
     1681 acgtcaggat tcagttgacc gtgcccggat tgtctttgaa caggcgcgga ttcgactcga
     1741 acgctatgaa cggctctatc acgaagggct ggtctcgcgc gacaaatatg atgagtatgc
     1801 cgccgcctac agcctggctg aaactgattt acaggccgct gaggctgccg ctgccgctca
     1861 aaaacggctg gtttcagccc agcaaaaaca gacggataaa acctcgaaag tcgcagccca
     1921 ggaagatgcg gcggatcaac gtcgccgtct tggcgcgctc gaaacggcaa ctcagcgttt
     1981 agagcgaacc gaagcggaac tcaagctggc ggaacaacgc ctgacggatt gcaccgtccg
     2041 cgccacagtt gacggtcacg tcattgccat cccggtcggc atcggagatc aggtagccag
     2101 tggcaacacg ctgatgacct ttgcccggct tgacacgctg gtggttcggg ttccagttgc
     2161 ctcaccattt attgatgccc tgcatgttgg cgacccggct caggtcatct tgccaacact
     2221 tccaccagaa acagttcccg gaaaagtggt taccct
//
LOCUS       DAOQDO010001015         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44716_length_2251_cov_3.810565, whole genome shotgun sequence.
ACCESSION   DAOQDO010001015 DAOQDO010000000
VERSION     DAOQDO010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44716_length_2251_cov_3.810565"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="murJ"
                     /locus_tag="PKZ53_15325"
     CDS             complement(<1..966)
                     /gene="murJ"
                     /locus_tag="PKZ53_15325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNJ41860.1"
                     /translation="MTHPSPPGTRNPEPGTRNAGFVGFAILSSRILGLVREQVFAILF
                     GAGLEYDAFVTAFRIPNLLRDLFSEGGLSTAFVTVFSQKLAKHGEAALWTLVNRVLHV
                     LLLLAGLVVLAGVWFQPQLMELIAPGFGAIPGKAELTVGLARLMWPFVGMVFLASLLM
                     GTLNAREQFAAPSFASASFNVGAIFGGLACAGWLAPEYVFGMARVVVGLQRPDHLDTS
                     HVALAIFGMAVGTLIGGTLQIVIQLPGLWSAGYRYAPTVSMTDPDVRRVLWLFLPAVI
                     GGAATQINVFINTYFASELGNGPVGWLNYSFRLIQFPIGIFGVAIA"
     gene            complement(1100..2179)
                     /locus_tag="PKZ53_15330"
     CDS             complement(1100..2179)
                     /locus_tag="PKZ53_15330"
                     /inference="COORDINATES: protein motif:HMM:NF021285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNJ41861.1"
                     /translation="MALLKDWIHRWEVHLSTRDRNRRADPFTWGLEHLADMNAYLPEP
                     FPFEDIALTDPHQFLMEYNAKVVANSDAFFTPTEVPEWHFHDNWLTFRSTVQTPYETN
                     NIAYARYFPVTTKKDSSGEMPPVVIVLPQWNADIRGHVAVCQMMNKLGMAAVRLSLPY
                     HDSRKPQNQMRADYMVSPNIGRTLQACRQAVHDVRSIITWLETLGYTRFGIVGTSIGS
                     CISFLSYVHDPRLRVGAFNHVSSYFADVVWTGISTRHVRDGLEGQVQRADLRNYWMAI
                     SPQPFVKRLRNEIHHGHKTLLISAQYDMTFLPHLSRQLMSEFERCRIPYQTAWMPCGH
                     YTSGEFPFKFYDGYLMGKHMVKYLK"
BASE COUNT          622 a          600 c          576 g          453 t
ORIGIN      
        1 ggcaatcgcc acaccaaaaa tgccaattgg gaactgaatc aatcgaaaac tatagttcag
       61 ccatccaacc ggaccatttc ccagttccga agcaaaatag gtattgatga aaacgttgat
      121 ttgcgtggca gcaccaccaa tcaccgccgg cagaaacaac cacagcaccc gccggacatc
      181 cgggtcagtc atcgaaaccg ttggagcata ccggtatcca gccgaccaca ggcccggcag
      241 ttgaatgaca atttgcaagg tcccaccgat cagcgttcca accgccattc cgaaaatggc
      301 cagcgcaacg tgactggtat ccagatgatc tgggcgttgc agaccaacca ccacacgggc
      361 cattccaaac acatactctg gggccagcca gccggcacag gcgagacctc cgaaaattgc
      421 gccgacgttg aaactggccg aggcaaatga tggcgcggca aactgttctc gggcattgag
      481 cgtgcccatc aggagtgacg ccagaaacac cattccgaca aatggccaca tcaatcgggc
      541 cagccccacc gtcaattcgg ctttgcccgg aatggcacca aaccctgggg caatcaattc
      601 catcaattgc ggctgaaacc agactccggc cagaaccacc agcccggcaa gcaaaagcag
      661 cacgtgcaga acgcgattga ccagcgtcca cagtgctgct tctccgtgct ttgcaagctt
      721 ttgtgaaaaa accgtgacaa aggccgttga gagaccacct tctgaaaaca aatcgcgcaa
      781 caggttggga atccgaaagg ctgtaacaaa agcatcgtac tccagcccgg caccaaacaa
      841 gatggcaaaa acctgctcgc gaaccagtcc taaaattcga ctgctcagaa tggcaaatcc
      901 aacaaagcca gcgtttcggg ttccgggttc cgggttccgg gttccgggtg gtgagggatg
      961 tgtcacaatt tttgaagctg ccttttttca cctgtcatcc aaatttcggg atccaagttc
     1021 caggttgaaa agacaaacgg gagaaaaaga ccaaaaggac aaaattttga aaacccggaa
     1081 cccggaacct gatggattcc tacttcaagt acttcaccat atgtttcccc atcaggtagc
     1141 catcataaaa cttgaacggg aattcgcccg aagtatagtg accacagggc atccaggcag
     1201 tctgatatgg aatccggcag cgttcaaact cgctcatcaa ctggcgtgac agatgcggca
     1261 ggaaggtcat atcgtattgc gctgaaatca aaagggtttt gtggccatga tggatttcgt
     1321 tgcgcagccg cttgacgaat ggctgggggc taatggccat ccagtagttg cgtagatccg
     1381 ctctttggac ctgcccttca agcccatccc ggacatggcg ggttgagatg ccggtccaga
     1441 cgacatcggc aaaatagctt gaaacgtgat tgaacgcccc aacccgcagc cgggggtcgt
     1501 ggacatagct caggaaactg atacacgagc caatgctggt gccgacaatg ccaaaccgtg
     1561 tatacccaag cgtttccagc caggtaatga tggatcggac atcatggaca gcctgacggc
     1621 aggcctgtag cgtccgacca atattggggc tcaccatata atcggcccgc atttgatttt
     1681 gcggtttgcg actgtcgtga tagggcaggc tcagccgaac cgccgccatt cccagcttat
     1741 tcatcatctg gcacacagcg acgtgtccgc gaatatcagc attccactgt ggcaggacaa
     1801 tcaccacggg tggcatttcc ccagaactat cctttttggt agtcacgggg aaataacggg
     1861 cataagctat attgttggtt tcataaggag tttgaaccgt gctgcgaaac gtgagccagt
     1921 tatcgtgaaa gtgccattcc ggaacttcgg tcggtgtgaa aaacgcatcg ctgttggcca
     1981 cgaccttggc attgtattcc atcaggaact gatgcggatc agtcagcgca atatcttcaa
     2041 acggaaaggg ttccggaaga taggcattca tatccgccag gtgttccaga ccccacgtaa
     2101 acgggtcggc ccggcgattg cggtcacggg ttgaaaggtg aacttcccag cggtgaatcc
     2161 agtctttgag taaggccata attcagaaaa tgaagcatga agaattgaga atgaagaatt
     2221 gagaatgaag aattgagaat gaagaatgaa g
//
LOCUS       DAOQDO010001016         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44770_length_2250_cov_2.697494, whole genome shotgun sequence.
ACCESSION   DAOQDO010001016 DAOQDO010000000
VERSION     DAOQDO010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44770_length_2250_cov_2.697494"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKZ53_15335"
     CDS             <1..866
                     /locus_tag="PKZ53_15335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41862.1"
                     /translation="TQFPDERLVQMMLGQVSMNSGNRVDAKAAFEKARQLDGTTARVY
                     GFLGNVALMDARFSEARELFKTALNKKVPGAAPFNAHYGLAYAEMYDGKPEKAIKHLE
                     AFYAEYIKTEAPKFFPPVFIWNSIARINLETGKAQEAMKAYEKGYESVPSSNLNDDDK
                     KTWLGRMHHGRARSLAKLGKFDEAWQEAELIKKMIEDGGEKGKQYWTSYHYVAGYIKL
                     EKGELPAAIEHLKQADQTNEFHRLLLARAYEKSGDKDNAKLIYTELAKQPLPNLETAL
                     AYPEARKKLKG"
     gene            complement(989..>2250)
                     /locus_tag="PKZ53_15340"
     CDS             complement(989..>2250)
                     /locus_tag="PKZ53_15340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41863.1"
                     /translation="STRLLTMGYGFVKMIQLLAWPAHLAADYDFSQIPLTHMLTLKAG
                     LSLALVGGLLVAGVILFQYEKITSFAIFFFFGTTLLVSNLIFPIGILIAERTLYFPSI
                     SICLIAGAILAESIRREGMWRLVAGCLCGVLIIGGIVRTMIRNLDWRDSMEFGTAMIR
                     DAPSNPKGYLVMGLYYLSQHRNAEAEVVLKKGIPLDLTRASLRNVLVDIYLREDRLEE
                     AQAEITEIFKINPHSPHGKRNLARLLVRQHKDQAAVAAYRETMTMVPILAENHHEFAV
                     LLSKTGDLKGAETEFLRAIELRPTFVEAYLNLGLTYRSLGKLDQAILCLKETIRLSPK
                     NDTAYALIGQIWLRQSRLDEAIHAFQQALALNPKCIDCYNNLGVALAQNGQQTEAEQA
                     FQQAIILNPNDSSAVQNLERLRQQLKP"
BASE COUNT          644 a          549 c          534 g          523 t
ORIGIN      
        1 caacccaatt tccagatgaa cgcctggttc agatgatgct gggtcaggtt tcaatgaaca
       61 gcggaaatcg ggttgatgcc aaagcggcct ttgaaaaagc gcgtcagctt gatggcacca
      121 ccgcccgggt ctatggattt ttaggaaatg tcgcgttgat ggatgcccgg ttcagtgaag
      181 cacgtgaact ctttaaaacc gcgctgaata aaaaagtccc aggtgccgct ccattcaatg
      241 cccactatgg tctcgcctat gcagaaatgt atgacggcaa accggaaaag gcgatcaaac
      301 acctggaagc gttctatgcc gaatatatca aaaccgaggc acccaaattc ttcccacctg
      361 tgtttatctg gaattcaatt gcccgcatca accttgaaac cggtaaggct caagaggcaa
      421 tgaaggccta tgaaaaaggg tacgaaagcg ttccgagcag caatttgaac gatgatgaca
      481 aaaaaacctg gctgggtcgc atgcaccatg gccgcgctcg ttcactcgcc aaacttggaa
      541 aatttgatga agcctggcag gaagctgaac tgatcaagaa aatgatcgaa gacggcggcg
      601 aaaaaggcaa acaatactgg acgtcctacc actacgtcgc cggctatatc aaacttgaga
      661 aaggtgaact cccagccgcg attgaacacc tcaaacaggc tgatcagacc aatgaattcc
      721 atcggctgtt gctggcgcgg gcctatgaaa aatcgggcga caaggacaat gcgaaactga
      781 tctataccga actggccaaa caacccttgc caaaccttga aacagccctc gcttatcccg
      841 aagccaggaa gaagttgaag ggataagcat cggtcatggc ttgaagatgg aagtccatca
      901 aagcaaaagg gaacctgagc aatcaggttc cctttcttct tgttcggggt tgggttacag
      961 ccagggccat ttatgacggt aggcggtact acggtttgag ttgctggcgg agccgttcca
     1021 ggttttggac agcacttgaa tcattcggat taagaattat ggcctgttga aatgcttgtt
     1081 cagcttcggt ttgttgtccg ttctgggcca acgccactcc cagattatta taacaatcaa
     1141 tgcactttgg attgagcgcc agcgcctgtt gaaacgcgtg aattgcctca tccagcctgg
     1201 attgtcggag ccatatctgt ccaatcagcg catacgcggt atcatttttg ggtgaaagac
     1261 gaattgtctc tttcaaacag agaatggcct gatccagttt tcctaaagat cggtaggtaa
     1321 gccccaaatt caagtatgcc tcaacaaaag ttggacgaag ctcaatggca cggagaaact
     1381 ctgtttcagc acctttcaga tccccagttt ttgaaagaag caccgcaaat tcatgatggt
     1441 tttcagccaa gattggtacc atagtcatcg tttcccggta tgcggcaacg gcggcttgat
     1501 ctttatgttg acgaaccagt aaacgagcca ggttacgctt gccgtgagga ctgtgcggat
     1561 ttattttgaa gatttcagtg atttcagcct gggcttcttc caaacggtcc tctcgaagat
     1621 agatatcaac cagcacattt cgcagagatg ctctggtcaa atcgagggga attcctttct
     1681 taagaacaac ctctgcttcc gcattgcgat gttgtgacaa ataatacaac cccatcacga
     1741 gatatccttt tgggttggat ggtgcatccc gaatcattgc cgttccaaac tccattgaat
     1801 ctcgccaatc caggttacgg atcatcgttc ggacaatgcc gccgataatc agtacaccgc
     1861 acagacaacc cgcaaccagt cgccacatcc cttcccggcg aatgctttcg gccaggattg
     1921 ctccagcgat gagacaaata ctaatcgaag gaaaatagag agttcgttcc gcaatcaaaa
     1981 ttccgattgg aaatatcagg tttgaaacca gaagggtggt accaaaaaag aaaaaaatgg
     2041 caaacgacgt aattttttca tactgaaaca agatcacccc ggcaaccagc aacccaccaa
     2101 ctaacgccag tgatagacca gcttttagag tcagcatgtg tgtcaacgga atttggctaa
     2161 aatcataatc tgccgcaaga tgggctggcc aggccagaag ctgaatcatt ttgacaaatc
     2221 catagcccat cgtgagcaat cgggtggacc
//
LOCUS       DAOQDO010001017         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44844_length_2248_cov_3.082079, whole genome shotgun sequence.
ACCESSION   DAOQDO010001017 DAOQDO010000000
VERSION     DAOQDO010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44844_length_2248_cov_3.082079"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1048
                     /locus_tag="PKZ53_15345"
     CDS             5..1048
                     /locus_tag="PKZ53_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41864.1"
                     /translation="MTPDIQAQQKAPQPQTQSREKYPQPPVDPREKHLGEIKQLTFGG
                     ENAEAYFSFDGKQLIFQSTRDGHPCDQIYSMNLDGSDLKLISTGYGRCTCSYFLKNGK
                     QILYSSTHVGSKDCPPKPDYSRGYVWSLYPDYDIFIANRDGSNPKPLTTTPGYDAEAT
                     ISPNGKKIIFTSDRSGDLELYSMDLNGKNVKQLTNTPGYDGGAFYSPDNKKIVYRAHR
                     PATEAILKDYQSLLKEHLIRPTTLEIFVMDADGKNQRQLTHNGKANFAPFFLPNSKQI
                     IFSSNMDDPKGRNFELYLINIDGTGLERVTYNDTFDGFPMFSPDGKKLVFASNRNSLT
                     QGDTNIFLVDWKP"
     gene            1119..1709
                     /locus_tag="PKZ53_15350"
     CDS             1119..1709
                     /locus_tag="PKZ53_15350"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNJ41865.1"
                     /translation="MTETHPDIDNNASTKTYYAMTAVGKDQPGIVAAVTRVLFELGCN
                     IEDSSHTLLGGEFAMILLLTVNSTVTRDRLESGLRDAGTPMGLSISVCPIEAAELIRA
                     NGSAHTLPFLISVYGSDQPGIIYRITQHLADRDVNITDMNTRVIRKDNAPTYVMMLEI
                     EVPSEVNPSHLTLELEGIGKELGVQVGVRPVEVAEL"
     gene            1709..2212
                     /gene="def"
                     /locus_tag="PKZ53_15355"
     CDS             1709..2212
                     /gene="def"
                     /locus_tag="PKZ53_15355"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNJ41866.1"
                     /translation="MAILNVLTIPDPRLKLMSHPVTSIDAAVDQFIKDFVETLQSFRG
                     CVGLAAPQVDNPIRIVAIDVSVSRKPHPNHGLMVMINPEIIASDGSELMREGCLSIPD
                     FTGNVRRSVQIALRYTDPTGKECNLETEGFEARAIQHEIDHLNGLLFLDRVSSLKDDV
                     FRRKNYL"
BASE COUNT          598 a          578 c          506 g          566 t
ORIGIN      
        1 atgcctgacg ccagatatcc aggcccaaca aaaagcaccc cagccccaaa cccaatcacg
       61 agaaaagtat ccccagccgc cagttgatcc acgggaaaaa cacctgggcg agatcaaaca
      121 gctcaccttt gggggcgaaa atgccgaggc ctatttttct tttgatggaa aacaactgat
      181 ttttcaatcc actcgtgatg gccacccctg tgaccagatt tactcgatga atctggatgg
      241 ctcagacctg aaattgatca gtactggcta tggacgctgc acctgctcct actttctcaa
      301 aaacgggaag cagattttgt actcatcaac ccatgtggga agcaaagatt gtccgcctaa
      361 accggattat tctcgtgggt atgtctggtc gctgtatcca gattacgaca tttttattgc
      421 caaccgggac ggatccaatc ccaaaccgtt gaccacaaca cctgggtatg atgccgaagc
      481 cacgatttca cccaatggaa agaaaatcat ttttacctct gatcgatctg gtgatctgga
      541 actctattca atggacctca atgggaaaaa tgtcaaacaa ctgaccaaca ccccaggata
      601 tgacggcggc gccttttatt cgccagataa caaaaaaatt gtctatcgcg cccaccgacc
      661 agccaccgaa gccatcctca aagactacca gtcgctgctc aaagaacatt tgatccgccc
      721 aaccacgctt gaaatttttg tcatggatgc tgatgggaaa aatcaacggc agttgaccca
      781 taacggcaaa gccaactttg ccccgttctt tctaccgaat agcaagcaga ttatcttttc
      841 ttccaatatg gatgacccaa aagggcgcaa tttcgagctt tatctgatca atattgatgg
      901 aaccgggctg gaacgggtaa cttacaatga cacctttgat gggttcccga tgttttcacc
      961 agatggcaaa aagctggttt ttgcttcaaa ccgcaacagt ttgacccagg gggataccaa
     1021 tatctttttg gttgactgga agccctagtg ccgtgagcag ccagtaacga gccataacga
     1081 cccattgcct tcaattttta taggaacttt ctatggtaat gaccgaaaca cacccagaca
     1141 ttgataacaa cgcctcaacc aaaacctatt atgcaatgac agcggttggg aaagaccagc
     1201 ccgggattgt tgctgctgtg acacgtgtgt tgtttgagct tggctgcaat atagaagatt
     1261 caagccacac gctgttaggc ggcgaatttg caatgatttt gctcctcacg gtcaacagta
     1321 ccgtcacccg tgaccgactt gaatccggcc tgcgtgatgc cggaacccca atggggctgt
     1381 ccatctcggt ctgcccaatt gaagcagccg agctcattcg ggccaacggg tctgcccaca
     1441 cgcttccatt tttgatttcg gtgtacggct ctgaccagcc aggcattatt tatcgcatca
     1501 cacagcatct ggctgatcgg gatgtcaaca ttactgatat gaacacacgg gtcatccgga
     1561 aagataacgc tccgacctat gtgatgatgc ttgaaatcga ggttccctct gaagttaatc
     1621 catcacattt gacactcgaa ctggagggaa ttgggaaaga attaggggtt caggttggag
     1681 ttcgcccggt ggaggtagct gaactgtaat ggctatcctg aatgtcctta caatccctga
     1741 tccacggctc aaactgatgt cacacccagt gaccagcatt gatgcggcag tggatcaatt
     1801 tatcaaggat tttgtcgaaa cgctccagtc ctttcgtgga tgtgtcggcc tggctgcccc
     1861 tcaggtagac aatcccattc gaatcgtcgc cattgatgtc tctgtcagcc gaaaaccgca
     1921 tcccaatcac ggcctgatgg ttatgatcaa tcctgaaatt atcgcctctg acggcagtga
     1981 attgatgcgt gaaggatgtt tgagtatccc tgacttcacg ggtaacgttc ggcgttcagt
     2041 tcaaattgct cttcggtata ccgatccaac cggtaaagaa tgcaatctgg aaacagaagg
     2101 atttgaagct cgtgcaattc aacatgaaat tgatcactta aacggacttc tcttcctcga
     2161 ccgggtttca tcactcaaag atgatgtctt tcggcggaaa aattaccttt agcatctggt
     2221 gcttcgtgct tcgtgcctgg tgcttcgt
//
LOCUS       DAOQDO010001018         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44888_length_2247_cov_3.010949, whole genome shotgun sequence.
ACCESSION   DAOQDO010001018 DAOQDO010000000
VERSION     DAOQDO010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44888_length_2247_cov_3.010949"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PKZ53_15360"
     CDS             complement(<1..1011)
                     /locus_tag="PKZ53_15360"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41867.1"
                     /translation="MLGLSSPGLALEPTKNITQYIHDVWQDELPQVSVLTVAQTKDGY
                     LWLGTYEGLVRFDGIQFTVVHPQNLNIPKSNGVFSLFEDSQGRLWIGTVGGGLLCLKD
                     GQFSGYSIQNGLPDNSVRAICEDQTGNIWIGTEDGLGLLENGKIRVFTTRDGLGSNVV
                     RTLCCDRSGKVWIGTIGGLSCFENGAFTCLTTTQGLPNNRVNAVYEDLEHTIWVGTEG
                     GGLSRWQNGHFQNYTVQQGLANNIILSVLEDRDGNLWIGTDGGGLNRMKKDGTIARFS
                     SKDGLSQNAVRSLFEDREGSLWVGTSSGLNRLRDGKFVTYTVQDGLSNNYLRTILEDR
                     QGT"
     gene            complement(1174..1830)
                     /locus_tag="PKZ53_15365"
     CDS             complement(1174..1830)
                     /locus_tag="PKZ53_15365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ41868.1"
                     /translation="MSSPSSHPDLPYGSIAKGYDRGMRFFEQHLFGWRRAELIPKATG
                     DVLELGAGTGVNLMLYTSARSLVLTDPDPLMLEKARTKPRLPGLEVQFIQAGAEHLPF
                     PDQSFDTVVATLVFCSIPRPTVALAEIRRILKPGGKLLMLEHIRPSPLLLGFVTDCLT
                     PLQKRLCGGCHLNRRTVSTVQAQGFSIQHVNFSKFDVVATLEATLSYPSSSPSAHWKT
                     "
BASE COUNT          568 a          581 c          567 g          531 t
ORIGIN      
        1 cgtgccctgg cggtcttcta aaatcgtccg cagatagtta tttgaaagcc cgtcctgaac
       61 cgtgtaggtc acaaactttc catcgcgaag ccgattgaga ccgctgctgg taccaaccca
      121 caaactgcct tcacggtctt caaacagtga ccgaacggca ttttgggaaa ggccatcttt
      181 ggacgaaaag cgagcgatgg tcccatcttt tttcatgcgg tttaagccac cgccgtcggt
      241 gccaatccac agattgccat cccgatcttc caacaccgac aaaatgatat tgttggcaag
      301 tccttgttga accgtgtagt tctgaaagtg accattttgc cagcggctca agcctccccc
      361 ctcggttcca acccagatgg tatgttcgag gtcttcatag actgcgttga cccgattatt
      421 tggcaatccc tgtgttgtgg tcaggcaggt aaaggcaccg ttttcaaagc aggaaagtcc
      481 gccaatcgtg ccaatccaga cttttccgct tcggtcgcaa cacagtgttc gaaccacatt
      541 gctccccagg ccatcgcggg tggtaaacac tcggattttg ccattttcga gcaatcccag
      601 gccgtcttca gtcccaatcc agatattgcc tgtttgatct tcgcaaatgg cgcgcacgct
      661 gttgtctggc agcccatttt gaattgaata accagaaaat tgcccgtctt tgagacacaa
      721 caacccacca ccaaccgttc caatccaaag tcggccctgt gaatcttcaa acagggaaaa
      781 gactccgttg cttttgggaa tgttcaggtt ttgcgggtga accacggtga actgaatccc
      841 gtcaaagcgg accagtcctt catatgtccc caaccacaga tagccgtctt ttgtctgggc
      901 aaccgtcaac actgagactt gtggaagttc atcctgccag acatcgtgaa tgtattgggt
      961 aatgtttttg gttggttcca gcgccaatcc gggcgaggaa agaccgagca cagacaccag
     1021 gagcaggatc acacgccaga cagggtgtgg tctgcagaaa cggcttatgg ggcgtgccga
     1081 atcctggctg ttcatggtag atggcctaat cgctgataga gtgaaatgtg aggtcgagac
     1141 cttgctcaaa gtgagttgaa agcctgaatt ctttcatgtc ttccaatgag cactgggtga
     1201 tgaactcgga taagaaagcg tggcttcgag ggttgccaca acgtcaaatt tagaaaaatt
     1261 gacgtgttgg atagaaaatc cctgggcctg aactgtggaa accgttcgac gattcaggtg
     1321 acagccaccg cagagccgtt tttggagcgg agttaaacaa tctgtgacga atcctaacaa
     1381 tagcggggaa gggcggatat gttcaagcat gagtaattta ccgccgggtt tcagtatccg
     1441 tcgaatttca gccagagcaa ctgttgggcg gggaatacta caaaacacaa gggtcgcaac
     1501 caccgtgtca aagctctgat caggaaacgg gaggtgttcg gcgccagcct ggataaactg
     1561 aacctcaagc cctggcaacc ggggtttggt ccgggctttc tccagcatca acgggtccgg
     1621 atcggtcagc accagcgagc gggcactggt gtaaagcatt aaattgacac ctgtccccgc
     1681 cccaagttcc agaacatctc cggtcgcttt gggaatgagt tcagctcgcc gccacccgaa
     1741 gagatgctgc tcaaagaagc gcattccccg gtcatatcct ttggcaattg aaccgtacgg
     1801 gagatcagga tgagaggatg gggaggacat agattgatga tcggtgacca aatgacagag
     1861 tgacaggtaa attgagttcc tcagaaaaat gttcagagca ccatctgtag acaaaaggat
     1921 tgcatcattc ctcccactca gcaaccctca cgcctgaagg cgttactttg gaaccgaaat
     1981 cattttctct ggaatgcaag agccctttca ggccaatggc attaagttaa gcaaaatatc
     2041 ttcacctggg agcgcgggcg tctcgcccgc agtcggttgg aattgaaatc caagcgggca
     2101 ggatgcccgc gctcccaccc ttaacttaat gccattgccc tttcaggctt ctcgtaagtc
     2161 ttcttgtgaa attgaagccg atgcccgatc accttgtcat ccggtacttg aaatcatagt
     2221 tgagaatgaa atcgagttct tcagccg
//
LOCUS       DAOQDO010001019         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44954_length_2246_cov_1.770881, whole genome shotgun sequence.
ACCESSION   DAOQDO010001019 DAOQDO010000000
VERSION     DAOQDO010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44954_length_2246_cov_1.770881"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..1094
                     /locus_tag="PKZ53_15370"
     CDS             465..1094
                     /locus_tag="PKZ53_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41869.1"
                     /translation="MKHQPGSAQPGLFDFLMDDPFSESANRPKPKPEPEPVTAKSEDP
                     PPPKPKPRAVPVPAPKPAPPPESKAVQTKPVKAPTGPKKTPPAPVVPLPEPRSTPQKQ
                     NSKLKAVPEPQPPAIEPPPQPPQLHQLSLTLPRKACPFCKREVLSLAKTTRPAQGMAV
                     ECLKCGFRGGVGVNEAEAVAKWDKDGLRNKGARLVQTLERSKKKSEGVK"
     gene            complement(1131..>2246)
                     /locus_tag="PKZ53_15375"
     CDS             complement(1131..>2246)
                     /locus_tag="PKZ53_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ41870.1"
                     /translation="HFPDFPMPESYPDFPSRQQMLEYLRAYAEHFNLRPQIQFRTKVV
                     SVQPHPNERWELEFESGEKRIYKGVLVCNGHHWDRRFPNYPGRFAGDYIHSKDYQHPD
                     QLKDKRVLVIGGGNSACDIASEAARIGTSCAISLRRGYWFLPKTVFGVPLVEFIPAWA
                     PLWLQRLVLKGILKVVVGNYEQYGLPKPDHNIFEAHPTLNSELLHYLKHGRIQPKPDI
                     ARFSGQTVEFVDGTQAEFDLVVCATGYHLSFPFLPPGLVPVTGSLATLYGGALLPEHK
                     HLYVIGTQQPRYGFGPLLTPSAQLLCQMIKVQDQMELPLGRVLKEFGVKPPASHLLDP
                     QKTLSDLRKARRFLPIFVWYERRLRRAKAQSRERQSV"
BASE COUNT          596 a          605 c          572 g          473 t
ORIGIN      
        1 cccgtacagg aaaattgaag gaatgtggcc aaagccagaa ccaaaaaacc gaaatttcgc
       61 aggctccgac gagcttgctc gtgagaaagt tgaccagaca taagcctcaa atgacctctt
      121 tgttgctcct gtaatttcag gaccaggaac gatttagaac caaaaagaaa taatttggat
      181 gaaacagtct attgcttgaa gtcgagtgga caattagtgt aagtgtagtg tcaaacaagt
      241 ttatagtcag tcgtcagtcg tcagtcgtca gtcgttgata agtcagtaga caaccccaga
      301 atgttttggt ttggaaattt tttggaaaga attgttcata actgactcaa atagaatcaa
      361 atatacttga aagataattc aagattccaa cactcgacga ctgacgactg acgactgacg
      421 actgacgact gacttctaaa gaaatttcca aacaggtagc gatcatgaag caccaaccag
      481 gttctgccca gccaggctta ttcgattttt tgatggatga ccccttttcg gaatcggcaa
      541 atcggcccaa acccaaacca gagccagaac ccgtgaccgc caaatctgag gatcctccac
      601 cgcccaaacc caaaccacgg gctgttccgg tgcccgctcc caaaccagcg ccacctccag
      661 agtcaaaggc ggtccagacc aaacctgtca aagctccgac tgggccaaag aagacaccgc
      721 cagcaccagt tgttccgttg ccagagccac gttcaactcc tcaaaaacag aactcaaagc
      781 tgaaagccgt ccctgaacca cagcctcccg cgattgagcc tcctccccaa ccgccgcaac
      841 ttcatcaact ctccctcacc cttccccgaa aagcctgtcc gttctgcaaa cgcgaagtgc
      901 tctcgctggc caaaaccacc cgaccagctc agggaatggc agtggaatgc ctcaaatgtg
      961 gatttcgcgg cggtgttggc gtgaatgaag ccgaagcggt ggccaaatgg gacaaggatg
     1021 gcctccgcaa caaaggcgcc cggctggtcc aaacgcttga acgatcaaag aaaaaaagcg
     1081 aaggcgtgaa gtagttcagt gttgatgctg aggagtggtc tatcttccct ctaaacgctt
     1141 tgcctttcac gactttgcgc tttcgctcgc cgtaatcggc gctcatacca gacaaaaatc
     1201 gggagaaacc gccgcgcctt gcgcaaatcg gacagggttt tttggggatc gagcaaatgc
     1261 gaagcgggcg gtttgacgcc aaattctttg aggacccgtc ccaacggcaa ctccatctga
     1321 tcctggactt tgatcatctg gcagaggagt tgggcactgg gagtcaaaag cggaccaaac
     1381 ccatatcggg gttgctgggt gccgatgacg tagaggtgtt tatgctcggg caacagcgcc
     1441 ccgccgtaca acgtcgccag gcttccggtg actggcacca ggccgggcgg caagaacgga
     1501 aagctcagat gatagcccgt cgcacagacc accagatcaa attcagcttg tgtgccgtca
     1561 acaaactcaa cggtttggcc tgaaaaccgg gcaatgtccg gcttgggctg gatgcgacca
     1621 tgttttagat aatggagcaa ctcgctgttg agcgtgggat gggcttcgaa aatgttgtgg
     1681 tccggtttgg ggaggccata ctgctcatag ttgccaacca cgactttgag aatgcctttg
     1741 agcaccaacc gctgaagcca gagcggcgcc caggccggaa taaattcaac cagcggcacg
     1801 ccaaacacgg ttttcggcaa aaaccagtac ccccgccgga ggctaatggc acaggacgtc
     1861 ccgatccggg cggcttcaga agcaatgtca caggctgaat ttcccccacc aatcaccagc
     1921 acgcgtttgt cttttaactg gtctggatgc tgatagtctt tggagtggat atagtcaccc
     1981 gcaaagcgcc ctggatagtt cggaaatcgg cggtcccaat ggtggccgtt gcacaccagc
     2041 acccctttat agattcgctt ttcacccgat tcgaattcca gttcccagcg ttcattgggg
     2101 tgcggttgga ccgacaccac tttggttcga aattgaatct gaggccggag gttgaaatgt
     2161 tcagcgtagg cgcgcaaata ctcaagcatt tgctggcgac tggggaagtc gggataactc
     2221 tctggcattg gaaaatccgg gaaatg
//
LOCUS       DAOQDO010001020         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_44965_length_2245_cov_3.624201, whole genome shotgun sequence.
ACCESSION   DAOQDO010001020 DAOQDO010000000
VERSION     DAOQDO010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44965_length_2245_cov_3.624201"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1448
                     /locus_tag="PKZ53_15380"
     CDS             222..1448
                     /locus_tag="PKZ53_15380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ41871.1"
                     /translation="MTVTPSFDVPDQRMRILVLTSSFPSREFPSTCRFVAEWVTSLSQ
                     FADVTVLAPPGAVLPDDLLASGVQLHCFAHLPESWEPQLRADTDLRRMLETKPAAWIE
                     FLPFHRKFYHQALCLAAQADVVCSHWLVPTAVIGHAVSRKRNLPHVMIEHSGGLRLLN
                     QIPGGLSLLKRMIDRSVGCITVSQELKTTLVERLPQVAEKTMVLPMGVADEALTARAW
                     SKAELRAALGLPHSGLVLLFLGRHVEIKGLPVVLKALENQPDVQLFVAGDGPCRTQWE
                     QQALKRGVTATFIGEVSGAEKWRWLAAADVMVVPSLEFKGGRTEGTPVVCLEALTVGT
                     PVMAAAVGGIPEHIQTGTNGWLLPAGDVAAWRAQINRLATDPAHLEEVSRVTRCQPAP
                     HVWSHRGREFAEILIR"
     gene            complement(1471..>2245)
                     /locus_tag="PKZ53_15385"
     CDS             complement(1471..>2245)
                     /locus_tag="PKZ53_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41872.1"
                     /translation="VDWQDVRTSADFQILQAFFEQSGYRTIICDPRAVEMRDGKLYHQ
                     DFRIDLVYRRVVTSELLEKFDECHDFVDAYRTHAACFINTFRCRISENKAFMEFLTDP
                     SHLGHLSADERLALDRYLPWTRHVSATKTDFHGQEIELGEFIAANREQFVLKPADSYG
                     GKDVYVGTEVEQSIWESAVHAAISQTRWVVQERVATPVEPFPVRVGDSFEFRDMKFNV
                     NPFYLGGVTCGAVTRTSTDAVINVSAGGGSVPSFTVSPR"
BASE COUNT          521 a          579 c          624 g          521 t
ORIGIN      
        1 aattgtgtgc ggttgaccgc acgaaccacc accttacttc aacgacggag cggcgtttcc
       61 tcccggccct gaaggaccgg gtctccacgc cgaagacagg atgaaaagtc agtaccacct
      121 gcgtcagcgg gtgggtttga aatcaagcat gaagaatgag aaagaatgca actctctatt
      181 tttcatagag atcagagttg attgaagaga atcaatccaa aatgaccgtg acaccttcat
      241 ttgatgttcc agaccagcga atgcgtatcc tggttctgac cagttccttt ccaagtcgtg
      301 aatttccctc aacctgtcgg tttgtggcgg aatgggtgac cagcctgagc cagtttgccg
      361 acgtgacggt tttagcacca ccaggggctg tcctgcccga tgatttgttg gcttcgggtg
      421 tacaactgca ctgttttgca cacttaccgg aaagctggga gccacagtta cgagctgata
      481 ctgatttgcg tcggatgctg gaaaccaaac cggctgcctg gattgaattc cttccatttc
      541 accgcaaatt ttatcatcag gcgctctgtc tggcggcaca agctgatgtc gtttgttcac
      601 actggttggt tcccaccgcc gtgattggcc atgccgtttc ccgaaaacgc aacctgcccc
      661 acgtgatgat tgaacattcc ggcgggctgc gattgctcaa tcaaattcct ggcgggttat
      721 ctcttttgaa gcgaatgatt gaccgttcag tcggctgtat cactgtgagc caggaattaa
      781 aaaccacgct cgtggagcgt cttccccagg tggcagaaaa aaccatggtg ctgccgatgg
      841 gagtggcaga tgaagcgctg actgcccgtg cgtggtcaaa agcagaactt cgggcagctc
      901 tgggtttgcc ccactcaggg ctggtgttgt tatttttagg gagacacgtt gaaattaaag
      961 gacttcccgt ggtgctgaaa gctttggaaa atcaacctga tgtgcagttg tttgtcgctg
     1021 gcgatggacc ctgccgaact caatgggaac agcaagctct caagcgaggt gttacggcga
     1081 cctttattgg ggaagtcagt ggtgcggaaa aatggcgctg gctggccgcc gccgatgtga
     1141 tggttgtgcc gtcacttgaa ttcaaaggtg ggcgaactga aggcacgcca gtggtgtgtc
     1201 ttgaagcgtt gaccgtcgga acacccgtga tggctgctgc tgtgggaggt attcccgaac
     1261 atattcaaac tggaaccaat ggttggctgc ttccggctgg cgatgttgct gcctggcgag
     1321 cgcaaatcaa ccggctggcg actgatccag cccacctgga ggaagtcagt cgtgtgacca
     1381 gatgccagcc agcacctcac gtctggtcac accggggaag agaatttgcg gaaatattga
     1441 ttcggtaggg atggacatat tggtccgccc ctaccggggc gataccgtaa atgacggcac
     1501 gctcccgccg ccggctgaaa cattgatgac ggcgtcggtc gaagtccggg tcaccgctcc
     1561 acaggtcacg ccgccgagat aaaacgggtt gacgttaaat ttcatgtcac gaaattcaaa
     1621 actgtctcca acccgcacag gaaatggttc gaccggagtt gccacgcgtt cctgaacgac
     1681 ccatcgtgtc tggctgatgg cggcgtggac ggctgattcc cagatgcttt gctcgacttc
     1741 ggtgccgaca tagacatcct tcccgccgta ggaatcagcc ggtttgagga caaactgctc
     1801 gcgattggcg gcgataaact ccccgagttc aatctcctga ccatgaaaat cggttttggt
     1861 ggctgacacg tggcgcgtcc acggcaggta gcgatccagg gcaagacgtt cgtcggcaga
     1921 taaatgcccc aggtgtgacg gatcagtcag aaactccata aaggctttgt tttcactgat
     1981 tcgacagcgg aatgtgttga taaaacaggc ggcgtgggta cggtaggcat caacgaagtc
     2041 atggcattcg tcaaattttt cgagcaattc actggtcacc acgcggcgat ataccagatc
     2101 aatccggaaa tcctggtgat agagcttccc atcgcgcatt tccaccgctc gcggatcaca
     2161 aatgatcgtc cgatagcccg attgttcaaa aaatgcctgc aaaatctgaa aatcagccga
     2221 tgtgcggaca tcctgccagt ccaca
//
LOCUS       DAOQDO010001021         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45038_length_2243_cov_3.219378, whole genome shotgun sequence.
ACCESSION   DAOQDO010001021 DAOQDO010000000
VERSION     DAOQDO010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45038_length_2243_cov_3.219378"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1480..>2243)
                     /locus_tag="PKZ53_15390"
     CDS             complement(1480..>2243)
                     /locus_tag="PKZ53_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ41873.1"
                     /translation="KELAVVADPVFEPTDARLPNHPSTSQGASPAPEKTDQTRKLILE
                     KTKASVEEVGLIDESKEIPRLLGTRREAESILKLVPKTKAAQAFDFAASRSFFDRPDL
                     DQFRLLHIATHGFVNSEHPELSGLIFSMVDQQGNDQNGFLLVPDVFNLKLNADLVTLS
                     ACQSGLGKEIKGEGMVGLTQGFLYAGTSRVLVSLWSVSDQATAELMKHFYHSLLVEKL
                     RPAEALRRAQLRLRQNKQWSSPYYWAAFQLTGEWK"
BASE COUNT          569 a          437 c          558 g          679 t
ORIGIN      
        1 ccggtaggtt caatgatacc gggttacctc tttttttgta aaatattgaa tatgtcgaga
       61 gcaatgttga gggcagttgg tgggctttta tagccgtgca cccttcactc atcgctcaac
      121 aactgctgac ctgaaaaaat acattcttat ggttttgaaa gaaccgctca ggtttttgtc
      181 agtggcccat tgaccagaaa acaaaagatt tgaataccga aaggcgtact gctttagtca
      241 ttgtggaatg gattattgcc cccagttgaa ggtccctggt acagaggatt cgtgttgccg
      301 ccggtccgtg ggggtgttgt ttcaggggtg gtttctccag ctttaacatt ggttttgatt
      361 tccatataaa tctctccagg taagtaaatt gtgtgatgaa tttgtactct tacaaagagc
      421 aacttcggtg ccatttcagc taaaaaggaa cattttcatt gtttaatatg aaaactattg
      481 cttttaaagg agatatcttg ttgaaatttt tgatgaggtt tggggtgaat tgaagcaaga
      541 gtgtgtattt agtatgcgga tgataagggg tggccctggt aacatactga attttaagtg
      601 gttgtgttgg tgttcagtgt gtgagcagtg tcaactcatt gagcatccaa aattcaactg
      661 aatggtcttc gggtgatgat ccttatttat ttccattctc gggtgagttg aaatgctgcc
      721 caaatcatag ggaaataaga atttttgatt gtgtagtaca tgaaaaacag gtttgaaaat
      781 aaaataaggt gtttcgacag gtttattacc ggaaacacct tatttgaggt ttgaagagtt
      841 ttggatgatt aaaaaaattg cttatatatt attctaagtt gcctgggttc tataagacag
      901 agagttatcc cttgtaaaga gggttgtcac ctgtctgacc agtgggtgta tataaaggat
      961 tgttcgtcac ttctgttgtt tgctctgacc ctgaaggttt ccctgacccg ccaagtactg
     1021 cccctgcttt gacatctgtt ttaatttcca ttgtaacctc tctggtgaac tgagtgtatg
     1081 attttctagt ggctttcaga agtgcaattc tcgtgccatc ataaggatac tcaaagtttt
     1141 ttttattctt gttagaagtc attgctagaa aaacagatag ctgcctgtgt gatggttggg
     1201 gtgatcgttg taattcagat ccctggtgtg tagttagtag acaaatctga gggaaggtct
     1261 atgcaaaata ttgattagaa agagagtatc caggtgttca ctctttgagc actggtaagt
     1321 agctgaacac tgaggggagt gctctgattg tgttgaaaat accttttcca atttgtcatc
     1381 gctgactgca agtaattgac tccatatatt aatcagaaaa attgcctcaa cctggctttt
     1441 tcaacttcaa cagttttgga gcacgaccaa aacttaggat tatttccact cgccagtgag
     1501 ctgaaacgcc gcccaataat agggcgatga ccattgctta ttctgccgca atcttaattg
     1561 tgcccggcga agtgcctctg ccggacggag cttttcaacc agaaggctgt gataaaagtg
     1621 tttcatcaat tcagccgtgg cctggtcact gacgctccac aaactgacga ggacacgtga
     1681 ggttccggca tagaggaagc cttgagtcaa cccgaccatg ccttcaccct taatttcttt
     1741 gcccagacca ctctgacagg cggaaagcgt gaccagatcg gcattgagct tgagattgaa
     1801 gacatccggt accagcagga agccgttctg gtcgttgcct tgctggtcaa ccatcgagaa
     1861 gatcaggccc gacagttccg gatgctcgct gttgacaaac ccatgcgtgg caatgtggag
     1921 caggcggaat tgatcaaggt ctggacggtc gaaaaagctc cggctggcag caaagtcaaa
     1981 cgcctgggcg gctttcgttt tggggaccag tttgagaatg gattcggctt cacggcgcgt
     2041 gcccaggagg cgtggaattt cctttgattc gtcaatcaaa ccaacttcct caactgaggc
     2101 tttggttttt tccagaatga gtttacgagt ctgatcagtt ttttcgggtg cgggtgatgc
     2161 cccttgtgaa gtagaaggat ggttgggcag tcgggcatca gttggttcaa ataccgggtc
     2221 ggccaccacc gccagttctt tag
//
LOCUS       DAOQDO010001022         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45087_length_2242_cov_2.305441, whole genome shotgun sequence.
ACCESSION   DAOQDO010001022 DAOQDO010000000
VERSION     DAOQDO010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45087_length_2242_cov_2.305441"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PKZ53_15395"
     CDS             complement(<1..1244)
                     /locus_tag="PKZ53_15395"
                     /inference="COORDINATES: protein motif:HMM:NF028457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HNJ41874.1"
                     /translation="MPGKEKPTGPKPYKEVITDQAKSRPGLFTIHQVGEKTYFELPEA
                     LYGKVMLWNTEVAKVPAGIEFGGFPVGYRVVKWERRNNKIQLRNVSYDKRADGKSAIQ
                     ESVDAANLEPIILTFDVEAEGENKSAVIEVTKLLSSDLPDFSAVSVLGSLGLSGPSQV
                     DSSRSYIEEIKPFPTNIETRSMITYSLGPPPNPSPNPNAPPPAQIPGDIRSISILVHY
                     SLVLLPEKPMMGRYVDPRVGYFTEGFEDYANDQNQVLLRRFITRYRLEKKDPKAALSE
                     PVKPIVYYIAREVPQQWRAYMKQGVEDWNVAFEAAGFKNAILCKDAPTKAEDPNWDPE
                     DARYSVIRWAAQRVENAMGPNVHDPRSGEIISAHIIFWQDLLKIFQKYYFVLCAPNDP
                     RAQKFPLPETVMGEAIRYVTAH"
     gene            1615..2202
                     /locus_tag="PKZ53_15400"
     CDS             1615..2202
                     /locus_tag="PKZ53_15400"
                     /inference="COORDINATES: protein motif:HMM:NF027619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ41875.1"
                     /translation="MARKTDTTSPTDKGEAILQAALELFSERGFHGTAMPLVSERAGV
                     SAGTIYRYFESKEALVNALYQKWKTSLLDHILDGFPEQSPFREQFHVFWQRYASFALE
                     FPMAFQFLELHHHAAYLDERSRLTEQRGHQRMVELFHENQRLQILKPFEVELLVAFVW
                     GALSGVLKAGLKGEIALTPEIIDAAEACCWEAIRR"
BASE COUNT          543 a          560 c          578 g          561 t
ORIGIN      
        1 tcgtgggcgg tgacatagcg aatcgcttcg cccatcaccg tttctgggag tggaaatttc
       61 tgcgcccgtg gatcattggg cgcacacaac acaaaatagt atttctggaa aatcttgagc
      121 aaatcctgcc agaaaataat gtgtgccgag atgatctcgc cgctgcgcgg atcgtgaaca
      181 ttgggaccca tcgcgttttc aacccgctgg gcggcccacc gaatcaccga gtatcgggca
      241 tcttccggat cccagttggg gtcttcggct ttggttggcg catctttgca caaaatggca
      301 tttttaaacc cggcggcttc aaacgccaca ttccagtctt cgacgccctg tttcatatag
      361 gcgcgccatt gctgcggcac ttcgcgggcg atgtagtata caattggctt gacgggttcg
      421 gacagggcgg cctttggatc tttcttttcc aggcgatagc gggtgataaa gcgccgcaac
      481 aagacctgat tctgatcatt ggcgtaatct tcaaaccctt cggtgaagta cccaacgcgt
      541 gggtcaacat agcggcccat cattggtttt tcaggcagca acaccagact gtagtgaacc
      601 agaattgaga tgctgcgaat gtcacctgga atctgagccg gcggtggggc attggggttg
      661 gggcttgggt tgggtggcgg tccaagtgaa taggtaatca tggaccgggt ttcaatgttg
      721 gtgggaaagg gtttgatttc ctcaatatag ctgcgtgaag aatcaacctg tgatggacct
      781 gataacccca aacttcctaa cacactgacg gctgaaaagt caggtaaatc agaggacagc
      841 aatttggtga cttcgatgac ggcagatttg ttttcgcctt cggcttcgac atcaaaggtc
      901 agaataatcg gttccagatt cgctgcatca accgattcct gaatcgcgct tttgccatca
      961 gcccgcttgt catacgacac attgcggagc tgaattttgt tgttgcgccg ttcccatttg
     1021 acgacgcgat atccgactgg aaagcctcca aattcaatcc cggctgggac tttggcgact
     1081 tcggtgttcc agagcatcac tttaccatac agggcttcag ggagttcaaa ataggttttt
     1141 tctccgactt gatgaatggt aaacaaccca ggcctggatt tggcctgatc ggtaatgact
     1201 tctttataag gcttcgggcc ggttggtttt tcctttcctg gcatttggcc aggtccctga
     1261 ggcatttcag ggatttgagc atggacagag gcggtcaaac acaacaccac ccccaaaaca
     1321 agagtcagac cccgcaagcg gggcgagcac acaggcttca ttctgttcct ccaaaaaagc
     1381 tgattttttt gattgatagg taaatgatgg ttggcggagt gtaacgaaaa aaaggaactt
     1441 taggatgcct tttcttgcga aagacaggga aatttctccg ctgaggaccc ggtcatcaat
     1501 ttgaccaatc gaaattgcaa agatgcagcc aggaaaaaga aagttcttga catccagcct
     1561 cctttacggt aaatgaatga acgttcattc caataaaaca caagttactc atttatggct
     1621 cgaaaaaccg acaccacctc acccactgat aaaggcgaag ccatcttgca ggccgcgctc
     1681 gaactgtttt ccgaacgtgg ttttcacggc acggccatgc cgctggtgtc agaacgggct
     1741 ggcgtctcag cgggtaccat ttatcggtac tttgaaagca aagaagccct cgtcaacgcc
     1801 ctctatcaga agtggaaaac ctccctgctg gatcacattc tggatggctt tcccgaacag
     1861 tcgccatttc gtgagcaatt tcatgtgttt tggcaacgct acgccagctt tgccctggaa
     1921 tttccaatgg ccttccaatt cctggaactc catcaccacg ccgcctatct ggacgagcgc
     1981 agccgcctga ccgagcaaag aggtcatcaa cgaatggttg aactctttca cgaaaatcaa
     2041 cgcctgcaaa tcctgaaacc atttgaagtt gaactcctgg tagccttcgt ctggggggcg
     2101 ctctcgggtg tgctcaaagc cggactcaag ggtgaaattg cgttgactcc ggaaataatt
     2161 gatgcggctg aagcctgttg ttgggaggcc atccgacgct aataccattt agggttcagg
     2221 gtatcgggtt cagggttcag ga
//
LOCUS       DAOQDO010001023         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45125_length_2241_cov_2.577768, whole genome shotgun sequence.
ACCESSION   DAOQDO010001023 DAOQDO010000000
VERSION     DAOQDO010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45125_length_2241_cov_2.577768"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..919)
                     /locus_tag="PKZ53_15405"
     CDS             complement(152..919)
                     /locus_tag="PKZ53_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ41876.1"
                     /translation="MKNRIKLAFSSVIVLLILNSQPTGAWINGGLTAQKPAQDMAPPQ
                     VIFHFSTLATVSPTKVLAGPPAADSKKLKKDFDTLLKWQANRTSKQIEQAQYEDKSFT
                     AYLFANVLGPQFTAEQLPMTDEFLKKVLHDAGDIAEAAKGIWKRPRPYHTDSRIEPCV
                     TRSKSFSYPSGSATLGRMWALVLADIFPKQRKALLKRGQEIGLNRVIGGAHFPSDVEA
                     GAKLADAMYRQLKKDPEYKKGLMAVKTEVSQKSPLSR"
     gene            929..1537
                     /gene="thpR"
                     /locus_tag="PKZ53_15410"
     CDS             929..1537
                     /gene="thpR"
                     /locus_tag="PKZ53_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNJ41877.1"
                     /translation="MTQKSLEMVRVFCAVDLSAEARKKAAEHITSLRQQVPESHAKWE
                     RPEKLHLTVKFLGDISTTDVAVLAHAAQTAAALIKPFDLALDGSGVFPLRNLARARVL
                     WLGVLDSTGRLGQLQAALETACADQGFAREERPFSPHLTLARLRGEKSDQTLARAHQS
                     TPFAKITFSVSELIVMKSELLPTGSRYTPLARCLLGPQSPKA"
     gene            2054..>2241
                     /locus_tag="PKZ53_15415"
     CDS             2054..>2241
                     /locus_tag="PKZ53_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41878.1"
                     /translation="MCLYPSRSSRTPSACPDSHAPRDQHSVSKQVGFFMELVKVTIDG
                     KEYQAPKGKLLIEVCKDQG"
BASE COUNT          502 a          585 c          601 g          553 t
ORIGIN      
        1 gcccatacgg gcgaaacccg acctttccgc aacggagttg caataaccgt tgccccgcat
       61 cgttcaagtt tctggtgaaa cttgaattgt tcaactgctc aatggaagca agctaaagcg
      121 tgagctctgt actttttctg aagacctgtg cttatcgaga aaggggtgac ttttggctga
      181 cttcggtttt gactgccatc agtccttttt tatattccgg atcctttttc aattgccggt
      241 acatggcatc cgccagtttg gccccggctt cgacatcgct cgggaagtgc gcaccgccaa
      301 tcacccgatt caacccgatt tcctggcccc gcttgagcaa ggctttgcgt tgcttgggga
      361 aaatatcagc caggaccagc gcccacatcc ggcccagagt ggcgcttccg ctcggatacg
      421 agaaactctt tgagcgcgtg acacagggtt caattcggga atcggtgtga tatgggcgcg
      481 gacgtttcca gatgcctttg gctgcttcgg caatgtcgcc ggcgtcgtgg agcacctttt
      541 ttagaaattc atcggtcatt gggagttgtt ctgctgtaaa ctgcgggccg aggacgttgg
      601 caaacagata ggccgtaaag cttttatctt catactgagc ctgttcaatc tgttttgatg
      661 tccggttggc ctgccatttg agcagcgtgt cgaaatcttt tttgagcttt ttggaatctg
      721 ccgccggggg accggcgagc actttcgttg gagaaacagt cgccagcgtt gaaaagtgaa
      781 atataacctg aggtggtgcc atatcctggg ctggcttttg ggcggtgagt ccaccattga
      841 tccaggcccc ggttggctgg ctatttaata taaggagtac tatcacagac gagaaagcca
      901 gtttgattcg atttttcatg gaaaacctat gacacagaag agtttagaaa tggtgcgtgt
      961 tttttgtgcg gttgatcttt cagccgaagc caggaaaaag gcagcggagc atatcacatc
     1021 cctccgacaa caggtccccg agtcacatgc gaagtgggaa cgacccgaaa agctccatct
     1081 caccgtcaag tttttgggcg atatttcaac cacggatgtc gcggtgttgg cccatgcggc
     1141 tcaaacggcg gcggccttga tcaagccctt tgacctggcc ctggacggat cgggtgtttt
     1201 tccactgcga aacctggcac gggcccgagt gctgtggctt ggggtgttag actcgacagg
     1261 gaggttgggg caattgcagg ccgcacttga aacggcttgc gccgaccagg gctttgcccg
     1321 cgaagagcgg cccttcagcc cacatttgac cctggcgcgg ttgcgtggcg aaaaatcgga
     1381 ccagactcta gccagagccc atcagagcac gccgtttgcc aaaattacct tttcagtttc
     1441 agaattgatc gtgatgaaaa gcgaattgct gccaaccggg tcccgctata cgccgctggc
     1501 gcggtgtctg ttaggaccac aaagtccaaa agcctaacgg caccggaggg taagcggaaa
     1561 tggccactga atcaaagcct ttggcttgaa ccccaatcaa atttcttcga ttgaagcttg
     1621 tcagccataa cttcagacgc tagtatacgc gcgcatcatt caaacccaag cacacactca
     1681 cctgggtagt ccatcatcac acgttcggta tcttgttcct atcacttact ttagagcggt
     1741 ttagtcagtc cttttggcga tggttttgcc cggtggtgca aactcgctcc tgtcttttgc
     1801 aaagccttct tccggaaaga gttttgatcg gtttcgaggg aaatttcaca gccagggtat
     1861 cttgtgcctg ggtgactgac ccacccgctg acccaggtgg gactgacttt ggtgactcaa
     1921 cacaacgaac cccacccgct gaggcaggtg gtactgaccc tggtcgtggt gctgggcgga
     1981 aggttgacta tacgaacccg tctgaatcgt gaaaagattt ccggttcacc tcagcatttc
     2041 agacaaccca gcgatgtgcc tgtacccaag ccgttcatcc cgaacgcctt cggcatgtcc
     2101 cgactcccac gctccacgcg accaacattc agtcagtaaa caggtaggat tttttatgga
     2161 actggtgaaa gtgacgattg acgggaagga gtaccaagct cccaagggga aactcctgat
     2221 tgaagtctgt aaagaccagg g
//
LOCUS       DAOQDO010001024         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45156_length_2240_cov_3.246224, whole genome shotgun sequence.
ACCESSION   DAOQDO010001024 DAOQDO010000000
VERSION     DAOQDO010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45156_length_2240_cov_3.246224"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKZ53_15420"
     CDS             <1..652
                     /locus_tag="PKZ53_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNJ41879.1"
                     /translation="CVDTVITIPNERLLHTVDRNTSLAEAFRTADDVLRQAVQGISDL
                     ITVPGLINLDFADVKTIMSGMGIALMGTGQARGETRALEATQRAIASPLLEEASIEGA
                     RGVLVNITGGKDLTLFEVNEASSIIREAAHEDANIIFGAVLDERMQDEMKITVIATGF
                     EQAAAALQQDPLAGLNRNVAGLRTGGLGSSMPRSGEGGGISRDDLDIPAFMRKKAD"
     gene            1048..1797
                     /locus_tag="PKZ53_15425"
     CDS             1048..1797
                     /locus_tag="PKZ53_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNJ41880.1"
                     /translation="MTGKRPFTYIRGVTDLNSNLLKEKRCEIINLLKTRGAMTVDALA
                     AELHLTKVSIRRHLELLEKDELVIYRTEWRERGRPHHLYSLSEKGENLFPKTYDVLAK
                     DLLSATQRCFGERGVLKVLSTRAEDMVAAILPQLEGLMFEARVEKLAKILLERGYLAE
                     CQPLPDGSYLLVERNCPTISIAYEFSQICHQEKRLYAELLGCEVIREQRIAGGDQVCA
                     YRILKPHSPQSTLETESSLGASALNGFKQSS"
     gene            1794..>2240
                     /locus_tag="PKZ53_15430"
     CDS             1794..>2240
                     /locus_tag="PKZ53_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNJ41881.1"
                     /translation="MSFWKTDKVNEQQVVEALRAVKDPDLHRDVVSLNMIRDIKIQGT
                     EVAFRFVLTTPACPVKEKLEDDARKAVLSIPGVTKVEIKMDSEVPKGRGIPEKEAIHG
                     VRNIIAVSSGKGGVGKSTVAVNLAVALGRTGARVGLLDTDIYGPNVP"
BASE COUNT          568 a          571 c          548 g          553 t
ORIGIN      
        1 atgcgttgac accgttatca caatccccaa tgaacgcctg ctccataccg tggaccgtaa
       61 cacatcgctg gccgaagcct ttcgaacagc ggatgatgtg ctccggcagg cagtccaggg
      121 tatctcagac ctgattacgg ttcctgggtt gatcaatctt gatttcgctg acgtgaaaac
      181 cattatgagc ggcatgggaa ttgccttgat ggggacaggt caggctcggg gtgaaacccg
      241 tgccctcgaa gccacacaac gcgccattgc cagcccgttg ctcgaagagg catctatcga
      301 aggcgctcgc ggcgtgctcg tcaatatcac cggtggcaag gatctcaccc tgtttgaagt
      361 caatgaagcc tcatccatca tccgcgaagc cgctcacgaa gatgccaata tcatctttgg
      421 ggcagtgctc gatgaacgta tgcaggatga aatgaaaatt accgtcattg caaccggctt
      481 tgagcaggcg gcagcggcgc ttcagcagga tccactggct ggactcaatc gaaacgttgc
      541 tggcttacgc acgggcggac tcggctcgtc aatgccacgg tctggcgaag gcgggggcat
      601 ctcacgtgat gaccttgata tcccggcttt tatgcggaaa aaagccgatt agcctttcac
      661 ctcttcaacc agtttgattg cccctcacaa cattttgcag ttcacaaccc cgcctgaata
      721 actcactgtt attcccgggg tcgttttttt gagatcgggg ctcaagacat tgggctgaag
      781 atttcgggct cagggtatta aacccgcttt cttcagcccg agcttgcgag tcttcagccc
      841 caagcccttt tttcagaaca atcggacaga tagagcgatt tccaatcatt agaatttctt
      901 aattttggaa agcttttctt ttctaaaatc gtgtaatatt gactttacaa taaatttact
      961 ttcttatcat ccactatttt tctctttaaa atcaatattt tatatattta aataatattt
     1021 ctatttaaaa aataaaaaat taacttattg acaggtaaac gaccgttcac ctatattcgc
     1081 ggggtgactg atcttaactc aaacctactc aaagaaaagc ggtgtgaaat catcaacctg
     1141 ctcaaaacgc gcggggcgat gacggttgat gctctggcag ccgaactcca tctgaccaag
     1201 gtcagcattc ggcggcacct cgagctattg gagaaagacg aactggtcat ctaccgcaca
     1261 gagtggcggg aacgcggacg tccacaccac ctgtattcgc tctctgaaaa gggcgaaaac
     1321 ctgtttccga aaacctatga tgtgctggcc aaagatttac tctctgccac acagcggtgt
     1381 tttggagaac ggggcgttct caaagtcctc agcacccggg ccgaggatat ggtggcggca
     1441 attttgcctc aacttgaagg actgatgttt gaagcacggg tcgaaaagct ggccaaaatc
     1501 cttttggagc gcggctatct ggctgagtgt cagccacttc ccgatggttc atacttactg
     1561 gttgaacgca attgcccaac cattagcatt gcctacgagt tttcgcaaat ttgccaccag
     1621 gaaaaacggc tctatgccga actgcttggt tgtgaagtca ttcgcgaaca acgtattgcc
     1681 gggggtgatc aggtatgtgc gtatcgcatc ctgaaacccc attcacctca atcaacactg
     1741 gagactgaat cttcattggg cgcctcggca ctcaatggat tcaagcaatc atcgtgagtt
     1801 tttggaaaac cgataaagtc aacgaacaac aggtagtcga agccttacgg gcggtgaaag
     1861 acccggattt gcatcgtgat gtcgtgagtc tcaacatgat ccgcgatatc aagattcaag
     1921 gcaccgaagt cgccttccga tttgtgctga ccacccctgc ctgcccggtc aaagaaaagc
     1981 tggaagatga cgcgaggaaa gccgttttgt ccattcccgg cgtcaccaag gttgaaatca
     2041 aaatggattc tgaagtcccg aaaggacgcg gaattccaga aaaagaagcc atccacggcg
     2101 tgcgcaatat tattgctgtc agttcgggca agggtggcgt tggcaaatca accgttgctg
     2161 tcaatctggc cgttgcgctt ggccgcacag gcgcccgggt tggattgctt gataccgata
     2221 tttatggccc caacgtgcca
//
LOCUS       DAOQDO010001025         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45158_length_2240_cov_3.012357, whole genome shotgun sequence.
ACCESSION   DAOQDO010001025 DAOQDO010000000
VERSION     DAOQDO010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45158_length_2240_cov_3.012357"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..749)
                     /gene="cysC"
                     /locus_tag="PKZ53_15435"
     CDS             complement(159..749)
                     /gene="cysC"
                     /locus_tag="PKZ53_15435"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNJ41882.1"
                     /translation="MYFQEKKMSQQKGFTIWFTGLSGAGKTTLTGVLVSRLASRGIRL
                     EVLDGDEVRTHLSKGLGFSKEDRDTNIRRIGFVCRLLARNGVGVISAAISPYRDIRNE
                     VRADIEQNGAEFIEVYVKCSLDALIERDVKGLYKKALTGEIKSFTGVSDPYEAPHSPE
                     VTVETDRETIEESAARILRELERRGLVPLAAAATGD"
     gene            complement(831..1547)
                     /locus_tag="PKZ53_15440"
     CDS             complement(831..1547)
                     /locus_tag="PKZ53_15440"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNJ41883.1"
                     /translation="MENATFELTSELTEAELTRVSAQFESAAPETILAWALEEFGQEV
                     ALATGFGAEGCVLVHMLAQINPTARMFYLDTGLLFPETYELKAKLETRYGVQIERRAS
                     RLSVDAQARLHGDRLWEREPNRCCQLRKIEPLVETLSDLRAWVTAIRRDQSVTRASSP
                     IVSFDSKFDLIKINPLATWNSQDVWRFIFKHKIPYNPLHDQQYPSIGCTPCTTPVQIG
                     EHSRAGRWRGTEKTECGLHR"
     gene            complement(1641..>2240)
                     /gene="cobA"
                     /locus_tag="PKZ53_15445"
     CDS             complement(1641..>2240)
                     /gene="cobA"
                     /locus_tag="PKZ53_15445"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNJ41884.1"
                     /translation="IDQARQGKRVVRLKGGDPFVFGRGGEEALALTTAGIPFEIVPGI
                     SSALAAPAYAGIPLTHRGLSSSFAVVSGARAGDGAMSETLVAETTGADTVVVLMGMAQ
                     LRSIVAEFIAAGRPATTPVAVIRWGTYDGQQKVIGTLETIAETAEQAQMKQPAVIVIG
                     DVVSLHAQLDWFPVKQPAQFAFLAETQGAVSLVSQTENT"
BASE COUNT          516 a          611 c          527 g          586 t
ORIGIN      
        1 ttcttcattc ttcattcttc attcttcatt cttcattctt cattcttcat tctttattct
       61 tcattcttca ttcttcattc ttcattcttc attcttcatt ccccggtgtc ctctgtggtg
      121 agtttctgtt tctgttcaaa ccaacctcgc aggaacgact aatctccagt cgcggcagcg
      181 gccagtggaa ccaacccccg gcgttccagt tcgcgcagga tccgggcggc actttcctca
      241 atcgtttccc ggtcagtttc aaccgtgact tcgggtgaat gtggggcttc atacgggtca
      301 gaaacacccg taaaactctt aatttcacca gttaaggctt tcttgtacag tccttttacg
      361 tcacgttcga tcagggcatc aagtgaacat ttgacataga cttcgataaa ttcagccccg
      421 ttttgctcaa tatcagcccg aacttcgttg cgaatatcgc gatatggcga aattgcggct
      481 gaaatgacgc cgactccgtt acgagccagc aggcgacaga caaagccaat gcggcggatg
      541 ttggtatcgc gatcttcttt cgagaaccca agtccttttg aaaggtgggt tcgcacttca
      601 tccccatcca gcacttcaag ccggatccca cggctggcaa ggcgtgaaac cagaacgccg
      661 gttaaggttg tttttccagc tcccgaaagt ccggtaaacc aaatcgtaaa tcctttttgt
      721 tgactcattt ttttctcctg aaaatagata ataaccacac gaaacccttt gaaaatgaag
      781 gttttatttt tcgtgtattt cgtggttcgt tgttctgaaa ttccaaatct ttagcgatgt
      841 aagccgcatt cagttttttc cgtaccgcgc cagcgaccag cccgcgaatg ctccccgatt
      901 tgaaccggtg tggtgcaggg tgtacagcca atggacggat attgttgatc gtgcagcggg
      961 ttatatggaa ttttgtgctt gaagataaat cgccacacat cctgactgtt ccaggttgcc
     1021 agcggattga ttttgatcaa atcaaatttt gaatcgaatg aaacaatcgg actgctggct
     1081 cgggtgactg attgatcccg gcgaattgct gtcacccacg cccgcaaatc ggaaagggtt
     1141 tcaaccagtg gttcgatttt tctcaactgg cagcaccggt ttggctcacg ctcccacaac
     1201 cggtcaccgt gaagccgggc ctgggcatca actgaaagcc gcgaagcccg ccgttcaatc
     1261 tggacaccat accgcgtttc gagttttgct ttgagttcgt aggtttcagg gaacaggagc
     1321 ccggtgtcaa ggtaaaacat ccgggccgtt ggattgattt gggccagcat atgcaccagc
     1381 acacatcctt cggccccaaa cccggtggcc agtgccacct cttgaccgaa ttcttcgagg
     1441 gcccaggcca aaattgtttc cggagctgcc gattcaaact gagccgaaac gcgggttaat
     1501 tcagcttccg tcaattcaga agtcaattca aacgttgcgt tttccatatt ttgtagttgt
     1561 ttctattctg agaaaatgta tccacggaat acaccgcacc acatcgcaaa aatcaaaaag
     1621 gcaaaacagg ctgaatgatt tcaagtattt tcggtttgcg aaaccaggga tacggcccct
     1681 tgggtttcag ccaaaaatgc aaattgggcc ggttgcttga ctggaaacca gtcaagctgg
     1741 gcgtgcagac tgaccacatc gccaatcaca atcacggctg gttgtttcat ctgggcctgt
     1801 tcggcggtct cggcaatcgt ttccagcgtt ccgatgactt tttgctggcc atcgtacgtt
     1861 ccccatcgga tgacagcaac gggcgtggtt gccggacggc ctgcggcgat gaattcagcc
     1921 acaatcgaac gcaactgagc cattcccatc aaaaccacca ccgtgtcagc tccggtggtt
     1981 tctgcgacga gtgtttcaga catggcgccg tcaccggccc gcgcaccact gacaaccgca
     2041 aatgaagatg aaagcccacg atgcgtcagt ggaattcccg cataggctgg tgccgccagt
     2101 gccgaactga ttcccggaac gatttcaaat ggaatgccgg ctgtcgtgag cgccagggct
     2161 tcttcgccgc cacgtccaaa cacaaacgga tcgccgccct tgagccgaac aacgcgtttt
     2221 ccctgccggg cctggtcaat
//
LOCUS       DAOQDO010001026         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45176_length_2240_cov_1.814645, whole genome shotgun sequence.
ACCESSION   DAOQDO010001026 DAOQDO010000000
VERSION     DAOQDO010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45176_length_2240_cov_1.814645"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="PKZ53_15450"
     CDS             complement(<1..2212)
                     /locus_tag="PKZ53_15450"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNJ41885.1"
                     /translation="MIDEQGRPSETEASVWVSREKRHFTNWIQDLEFELIPDKKEYQP
                     GETAQILVNSPFLPAHGVMTLERDGIITYHPIELNESSQVLHVPLEDNFIPAIRVSVY
                     LSGEIVRADEDGTELPQLPKCPVSGTGSIQIPVSLRSRTLSVTAKPRVDQLAPGSATT
                     INLEVRDHTGKPVPESECVVAVVDEAILALFNGEIEDPVKVMYRDQLFGSKFQTLRDS
                     IRRASESELTADITSLLQVRPAANRFLPITSYALKRQARIKTEESPGKNREAGPQFDL
                     AQDNIATLNQSDQSIPLRSNFDPLAVFAPSVVTDADGKAVVTVTMPDNLTRYRVIALA
                     TDGEKRFGKGESTITTRLPLMVRPSAPRFLNFGDEFELPVTIHNQTDQAQHVNVAVQS
                     GQMNFPQGAGHLVLVPAHDRVEVRFPALPQTTGTVFFQVVASSDLASDAASGSFPVKP
                     PTMTETFATYGELQAGTLQLSLTIPTDARPEFSQFEMTISATQLQTLTDAVLYLMDYP
                     YGCTEQLASRILAITALQEVLTELRAPGQPTPEAYQTQVAADLDQLETVHNLDGGFGF
                     WSRSEKPLPFLSIHVAHALIRAKQAGYTVPYFLLGRSKDYLSKIKDHIPKEYSSESRQ
                     TLLAYALYVRHLNGESDADAARDLVKETGADKLPIEAIGWLLPVLAKNPATKSEQNTL
                     VRVLTNRVTETAGAASFVESYSDGAAVLLHSNRRTDAVILDALLSVQPDSDLIPK"
BASE COUNT          507 a          546 c          647 g          540 t
ORIGIN      
        1 gttttggaat caggtcgctg tctggttgta ccgaaagcag ggcatcgagg atgaccgcat
       61 cagtgcggcg gttggaatgc aagagcactg cagccccatc ggaatacgat tcgacaaagc
      121 tggcggcacc agcggtttcg gtgacacggt tagttagaac ccgaaccagt gtgttctgtt
      181 ccgactttgt agccggattt ttggccagca ccggcaataa ccagccaatg gcttcgatag
      241 ggagtttgtc agcaccagtt tctttgacca gatccctggc cgcatcagca tcgctctcac
      301 cattcaaatg ccgaacatac agcgcataag ccagcagcgt ttgacgtgac tcagagctgt
      361 attcttttgg gatgtggtct ttgatcttcg aaaggtagtc tttggaacgt cccagcaaaa
      421 aataaggaac ggtatacccg gcttgtttgg ctcgaatcag ggcatgggcc acatgaatgc
      481 tgagaaaggg cagcggtttt tcactccgag accagaaccc aaatccacca tcaaggttgt
      541 gaactgtttc aagctggtcg aggtcagccg cgacctgtgt ttgatatgct tccggggttg
      601 gttggcctgg tgcgcgaagt tcggtcaaaa cttcttgcaa ggcggtaatg gccagaattc
      661 gagatgcaag ctgctcagta cagccatagg gatagtccat caggtacaac acggcatccg
      721 ttagcgtctg gagttgggtt gcggagattg tcatttcaaa ctgactgaat tcggggcgcg
      781 catcagtggg aatcgtcaac gacaattgaa gcgttcccgc ctggagttcc ccataggtcg
      841 caaaggtttc ggtcatcgtg ggcggtttca ccggaaagga cccgctggca gcatcactgg
      901 ccaggtctga actggccacg acctggaaaa agacggtgcc tgtggtttgc ggaagagctg
      961 gaaatcggac ttcaacccgg tcgtgagctg gaaccaaaac caggtgaccg gcaccctggg
     1021 gaaaattcat ttgcccggat tgtacggcca cgttgacgtg ctgagcctgg tcggtttgat
     1081 tgtggatcgt aaccggaagt tcaaactcat caccgaagtt aagaaatcgt ggcgctgatg
     1141 ggcgcaccat caacggaagc cgcgtcgtga tggttgattc acctttgcca aagcgttttt
     1201 ctccgtcagt tgccagggca atcactcgat accgggtcag gttgtcaggc atggtgacgg
     1261 tgaccactgc tttaccgtcg gcatcggtta ccaccgacgg ggcaaaaacc gccagcggat
     1321 caaaattgga ccgcaatgga attgattggt ctgactggtt tagggtggcg atattgtctt
     1381 gtgccaggtc aaactgaggt ccagcttcac gatttttgcc tggggattct tccgttttga
     1441 tacgggcttg acgcttgagc gcatatgacg taatcgggag aaaccggtta gcagcgggcc
     1501 tgacctgtag caggctggtg atatcagccg ttaattctga ttccgaggct cgacgaattg
     1561 agtcacgaag ggtttgaaac ttacttccga acaactggtc acgatacatc accttgactg
     1621 gatcctcgat ttcaccgttg aacagcgcca ggatggcttc atccaccact gcaaccacac
     1681 attcactttc tggaactggt ttcccggtat ggtcccggac ttccagattg atggtggtcg
     1741 cggaacctgg tgcaagctgg tcaacccgtg gtttggcggt gacagagagg gttcgggagc
     1801 gcaacgagac cggaatctga atcgaaccag tgccggagac cgggcatttc ggaagctggg
     1861 gcagttcggt gccatcttca tcagctcgaa ctatttcgcc gctcaggtaa accgaaacgc
     1921 gaatggctgg aatgaagtta tcttcaaggg gaacgtgtag cacctgggat gattcattga
     1981 gttcgatggg gtgataggtg atgatgccgt cccgttcgag ggtcatcacg ccgtgggccg
     2041 ggagaaacgg tgaattcacc agaatctggg ccgtttcacc tggctggtat tcttttttgt
     2101 ctggaatgag ttcaaattcc agatcctgaa tccagttggt aaagtgtctt ttttcacgcg
     2161 agacccagac cgaagcttcg gtttcagagg ggcggccttg ttcgtcaatc acgcgggctc
     2221 tgatgacaaa ttcccctcct
//
LOCUS       DAOQDO010001027         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45219_length_2238_cov_3.733394, whole genome shotgun sequence.
ACCESSION   DAOQDO010001027 DAOQDO010000000
VERSION     DAOQDO010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45219_length_2238_cov_3.733394"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKZ53_15455"
     CDS             complement(<1..643)
                     /locus_tag="PKZ53_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41886.1"
                     /translation="MRCITWLLVWLFCGSLGIASLEVSAQQPPSRCEVLTPRTNVSVQ
                     IVSNGYPKPVYYDGSYRWIEGRPGERFVFQITNHNGFAVGVIPSADGHSLTSDGRASA
                     SHPAYLIEPYGTITVSIWRETLAGGRELFFTSVDNSLAARKGDRRNIGVLGVLVWQLE
                     DRAQIIVPNRKDGRGEEKSKAGAPAAGDSDQIYNSQGIGVGAGQYQSDQAALTD"
     gene            complement(859..>2238)
                     /locus_tag="PKZ53_15460"
     CDS             complement(859..>2238)
                     /locus_tag="PKZ53_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNJ41887.1"
                     /translation="KAYTDGETVKGRVVERIKGGLRVDLGGVSGFLPGSQVDVRPVRN
                     LDALKGREMDLRILKVNKKRSNIVLSRKVVLEEEASKKKDHTLELLDDDVIVTGQVKN
                     ITDYGVFVDLGGIDGLLHITDISWGRLQKPSDVFKVGDSIQVKVLKFDRDKGRVSLGY
                     KQLLPDPWDTVYERYHKGDTVRGQVASVTDYGAFIELEEGVEGLVHVSEMSWSKRIKH
                     PSKIVAPGTDVEAVVLEVDQSQRRISLGMKQVLPNPWDSVTERYAIGTKVTGRVRNLT
                     EFGAFVEIEDGIDGLVHVSDMSWTKRIKHPSEALKKGQQVEAVITNIDVNNRRLSLSI
                     KDLEPNAWDRFFQVHKPGDVVVGKITRFASFGAFVELEGGIEGLCHVSELSDQRVDKP
                     ESVVNIGQEIQFKILKLDESQKKIGLSARAVGRENDPEDVSNYINNETMTSLGELTNF
                     LGDPEEKES"
BASE COUNT          521 a          635 c          517 g          565 t
ORIGIN      
        1 gatctgtcag ggcagcctga tccgattggt actggccggc gccgaccccg atcccctggg
       61 agttgtaaat ctgatccgag tccccggcgg ctggtgcccc agcctttgat ttttcctcgc
      121 cgcgaccatc cttccggttt ggaacaataa tttgagcccg atcttccagt tgccagacca
      181 gtaccccaag cacgccgatg ttgcgacgat ctcctttccg agcagccagc gaattatcca
      241 cactggtaaa gaaaagctct cttccaccag ccagcgtttc gcgccagatt gagaccgtga
      301 tggttccata gggttcaatc agataggctg ggtggctggc tgaagcccgg ccatctgatg
      361 tcaatgaatg tccatcggct gaggggataa ccccaaccgc aaacccattg tgattggtga
      421 tttgaaacac aaaccgctct ccaggccgcc cttcaatcca gcggtaggag ccatcgtaat
      481 acaccggttt tgggtatccg tttgacacaa tctggactga aacattggtc cgaggggtga
      541 ggacttcgca tcgggacgga ggttgttggg ctgatacctc aagtgaggca atgcccaaac
      601 ttccgcaaaa caaccaaacc aacaaccagg taatacagcg catacatccc tgcctttcgc
      661 tcgctgattt tcacattcaa tcgggctgaa atgatctgaa ttttctgaac agtgtgaacg
      721 tattgtcgcc tgatggcctg aatcgttcca caacgactgg atgaaatcct gcaaaaaccc
      781 cacaaaacaa cacaagccgc ccaggtttga tacctgagcg gcttgtgtca tttctacttt
      841 tcgagccaga gaactcgatt agctttcttt ttcttctgga tcacccagga agttcgtcag
      901 ttctccgagg ctcgtcatgg tctcgttgtt gatgtagttt gatacatctt ccggatcatt
      961 ttcccgaccc acagcacgcg ctgaaagacc aatcttcttc tggctttcgt caagcttcag
     1021 gatcttgaac tgaatctcct gaccaatatt gacaaccgat tcaggtttat caacacgttg
     1081 gtcactcaat tcagaaacgt ggcacaaccc ttcaatgccg ccttcaagtt ccacaaaggc
     1141 cccaaagctg gcaaagcggg tgatttttcc aaccaccacg tcccctggtt tatgcacctg
     1201 gaagaaccga tcccaggcat ttggttcgag atctttgatc gagaggctga gacgtcggtt
     1261 gttgacatca atgttggtaa tgacggcttc aacctgctgt ccttttttca gcgcttcaga
     1321 tggatgcttg atgcgcttgg tccaggacat atccgacacg tggaccaggc cgtcaatgcc
     1381 atcttcaatt tcaacgaatg caccaaattc ggtcaggttg cgcacccggc cagtgacttt
     1441 ggttccaatt gcataccgtt cggtgactga atcccatgga tttggcagaa cctgcttcat
     1501 gccaaggctg atacggcgtt gtgactggtc aacttcgagg accaccgctt cgacatcagt
     1561 acctggcgcc acaatctttg acgggtgctt gatccgtttg gaccagctca tttctgaaac
     1621 atggaccaga ccttcaaccc cttcttcaag ctcaataaag gccccatagt cagtcactga
     1681 agccacctgg ccacggacgg tgtccccttt gtgatagcgt tcgtagacgg tatcccatgg
     1741 atctggcagc aattgcttgt agcccaggct gacgcggcct ttgtcgcggt caaacttgag
     1801 gactttgacc tgaatgctgt ctcccacttt gaacacatca cttggtttct gaaggcgtcc
     1861 ccaggaaatg tcagtgatat gcaacaaccc atcaatgccg ccgagatcaa cgaaaacacc
     1921 ataatcggtg atgtttttca cctgaccggt cacaatgacg tcatcgtcaa gtaactctaa
     1981 ggtgtggtct ttcttcttgg aagcttcttc ttcaagcacc actttgcgag aaagaacaat
     2041 gttgctgcgc tttttgttga ctttcaaaat gcgcagatcc atttcgcggc ccttcaaggc
     2101 atccaggttc cgcaccgggc ggacatcaac ctggcttcct ggcaaaaagc cgctcacacc
     2161 gccgagatca acacgcagcc cgcctttgat ccgttcaacc acccgtccct tgacggtctc
     2221 gccatcggta taagcttt
//
LOCUS       DAOQDO010001028         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45233_length_2238_cov_2.760880, whole genome shotgun sequence.
ACCESSION   DAOQDO010001028 DAOQDO010000000
VERSION     DAOQDO010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45233_length_2238_cov_2.760880"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /locus_tag="PKZ53_15465"
     CDS             <1..2188
                     /locus_tag="PKZ53_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNJ41888.1"
                     /translation="TEARLSPGPTQSSPIIINNSDSFVWVVNPDNNSVSILEVRADAN
                     RKVREVPVGKEPQSLAFNADNSKVYVANALDGTVSVISTSTFTVVKTIPVGTEPWALA
                     FTPNGTKLYVVNSYSKNVSVINPQTDTVVKTIEIPNAPLGERTIPRAITISNNGDFDD
                     TDEKVYVCNFLGEDLPNRVIGSDNYKQGRVNIISSGTDQLIGSITLAPLAVTGFKSDG
                     AAIAKIPPKGGGVFEVDTGAFPNQLTAFTIKNDKAYVPSQGASPEGPVRFNVILQPLI
                     STIDLGAEREIGEPLNANRGFQFEPPKEVTVNGQQIQFHRFNTIPMALVFKRTENVGY
                     LVLGGSDCLLRVELDSAGRPSVLAPAAAGDPGNFERILVGKNPRGMVMNSTDTRGYVF
                     NYISRDVTVIDLTKDARQVNNVIGSIRAADLPAAGSREATILLGKELFNTTIGPMQPR
                     AAKNGVLEGAMADFGWCGCYACHPEGLTDGVVWMFPDGPRRSIPLNQTFNPRDPSDQR
                     ILNWSAVRDEVQDFELNTRGVAGGQGLILLANGNQDPEVFNLLPRASSGRDPRQDSIN
                     EYVKTIRSPLSFVPTTDKDAAKGRKLFEKAGCVDCHAGGKWTSSKLDFTPAPPTDRVS
                     ADGEITSVLRKVGTLDVNNPNEVIGTGANISKPAKGAAGFNPASLLGTFALGPYLHNG
                     EAQSLSEVLNNATHTGPKATKTLGNAKKRAQLLRFLESIDDRTTPF"
BASE COUNT          568 a          625 c          562 g          483 t
ORIGIN      
        1 gaccgaagcc cggctgtcac ccggtccaac ccaatcaagt ccaattatta ttaacaatag
       61 cgatagtttt gtctgggtcg ttaaccctga caacaactcc gtttcaattc ttgaagtgcg
      121 cgctgacgcc aaccggaaag tccgcgaagt accagttgga aaagaacccc aaagcctggc
      181 gtttaacgct gacaattcca aagtttatgt cgccaacgcc ctggatggca ccgtgtcggt
      241 catcagcaca tccaccttca cggttgtgaa aacaattccc gtcgggacag aaccctgggc
      301 actggcattt acccccaacg gcaccaagct gtatgtggtg aattcgtatt ccaagaatgt
      361 gtcagtaatc aatcctcaaa ctgacaccgt ggtaaaaacg attgaaattc caaatgcccc
      421 actgggtgaa cgcaccattc cacgtgccat taccattagc aataatggtg atttcgatga
      481 tactgatgaa aaggtctatg tgtgcaactt cctcggtgaa gatctgccca accgggtcat
      541 tggttctgac aattacaaac agggccgggt caacattatt tcatctggaa ccgaccagtt
      601 gatcggctca atcacgctgg caccactggc tgtgactgga tttaaatctg acggtgccgc
      661 gattgccaaa attcccccca aaggtggcgg cgtgtttgag gttgataccg gggcatttcc
      721 aaatcagctc acggccttca cgatcaaaaa cgacaaagcc tatgtcccca gccagggggc
      781 ttcacccgaa ggcccggttc gctttaacgt catccttcaa ccgttgatca gcacgattga
      841 tttaggggct gagcgtgaaa ttggcgagcc actcaatgcc aatcgtggat ttcagtttga
      901 accccccaaa gaagtcaccg tcaatgggca acaaattcaa ttccaccgct ttaataccat
      961 cccaatggca ctggttttca agcgaacgga aaatgtcggc tatctggtcc ttggcggcag
     1021 tgactgtctg ctccgggttg aactcgatag tgctggtcgt ccaagtgttc tggctccagc
     1081 cgccgccggg gacccaggca attttgaacg aatcctcgtc ggtaaaaacc cacgtggcat
     1141 ggtgatgaat agcaccgaca cccgcggcta tgtgttcaac tacatttcac gcgacgtgac
     1201 cgtgattgat ctgaccaagg atgcccgtca ggtcaacaat gtgattggtt caattcgggc
     1261 agcggatctg cccgccgctg gttcacgcga ggccaccatc ctgcttggaa aagaactctt
     1321 caacaccacc attggcccaa tgcaaccacg agcggccaaa aacggagtgc ttgaaggcgc
     1381 catggctgac tttggctggt gcgggtgtta tgcctgccat ccagaaggtc tgaccgatgg
     1441 tgtggtctgg atgttccccg atggcccacg gcggtcaatc cccctcaacc agacctttaa
     1501 cccacgcgac ccaagcgacc agcgaatttt gaactggtca gccgtccgtg atgaagttca
     1561 ggactttgaa ctcaacactc gcggcgtggc tggcggtcaa ggtctgatcc tgcttgccaa
     1621 tggaaaccag gatccagaag tgtttaacct gctgccccgc gcgagcagtg gccgtgatcc
     1681 acgccaggat tcgatcaatg aatatgtgaa aacaatccgc agcccgctct cctttgtgcc
     1741 aacgacggac aaggacgcgg cgaaaggtcg gaaactgttt gaaaaagccg ggtgtgtgga
     1801 ttgccacgcc ggaggcaaat ggaccagcag caagcttgat ttcaccccgg cacctccgac
     1861 tgatcgggtg agcgctgatg gcgaaatcac cagtgtgctc agaaaagttg gaacgcttga
     1921 tgtcaataac cccaacgaag tcatcggaac tggggccaat atcagcaaac cagccaaagg
     1981 tgcggctggt tttaatccag ccagcctcct cgggacattt gcccttggac cgtatctgca
     2041 caatggtgaa gcacaatcac tcagtgaagt gctcaacaat gccacccata ccggccccaa
     2101 agccaccaag acgctgggga atgcgaaaaa acgggcccaa ttgcttcgat tcctcgaatc
     2161 aattgacgac cgaacaactc cattctaatc gggcttaggg cttggggttg aagactcgca
     2221 agctcggggc tgcaaaac
//
LOCUS       DAOQDO010001029         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45244_length_2238_cov_2.510765, whole genome shotgun sequence.
ACCESSION   DAOQDO010001029 DAOQDO010000000
VERSION     DAOQDO010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45244_length_2238_cov_2.510765"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..>2238)
                     /locus_tag="PKZ53_15470"
     CDS             complement(58..>2238)
                     /locus_tag="PKZ53_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNJ41889.1"
                     /translation="GESKIFWLYFVFTALGGPLWGLFLGLTSSLRLVGFQFTSGETPN
                     RIGKFELPGHWRQRVLGMAAVFAFFGVLAVLFPAYFSDPLYGMFAGATEKMWAFRLVF
                     GHRKISTALILAGLTFLFCVLDELATTYLLPNSQETPSPKWSHRLAKLLNPQILYPVL
                     ALAGLWLLVVSYFADANIMVTRRDLSFHVPMYLTALTSSLILSAILYRLVIVTRFGGA
                     VIGVLVLAVVGASVFALQQYETNQNLKSLFWRRGVVAKRYVNFARSLTDGEQKDFTSV
                     IAKNIPPPVKTDTHDLIPVPPVSAAEKKDQTGSGAAASQSIKQTLTSALSTATTALGG
                     SVTTPLSTAATGPRNVIFLSIDTLRADHMSIYGYKRHTTPNLDRFAKQSIFCEHGYSS
                     GTNTGHSFSSIMRSAQGDGIFDNSVPTVAKLFADKGYQTAFITSPKTKTWLYKGRWFE
                     YKQIMMESYQEITHEEARFWKADEMTDRSIDLLKRMKDKGPFFAWIHYTDPHAPYEAH
                     AECGYGETDPDIYDSEITFTDLHIGRLLSYLQESGLLNNTLIAFTSDHGEGFNEHGAL
                     EHGCLPYIEQVFVPFWVYAPGAEPKRLTMPVSHVDIAPTMLAYAGITPPNQYEGIDIV
                     KLARGEVAAHPHVICETPRNIPEGTFFAWSLIEGEWKIIYDRVGSNWQLYHLKQDPME
                     QRNLADLEPQKFSDLKQKLGQYLARQAQRKDYSNWRRFEFRFGS"
BASE COUNT          542 a          574 c          598 g          524 t
ORIGIN      
        1 cagccctcag ccccaagccc gaagtcttca gccctggttt tttcagccct ggttttttca
       61 gcttccaaac cggaattcaa accgccgcca gttggaatag tctttgcgtt gagcttgcct
      121 ggccagatat tggccaagtt tctgttttaa gtcagagaat ttttggggtt ccaggtccgc
      181 cagatttcgc tgctccatcg gatcctgttt taaatgatag agttgccagt tgcttccgac
      241 gcggtcgtaa atgattttcc attcaccctc gatcagcgac caggcaaaga aggtgccttc
      301 cggaatattg cgcggtgtct cgcaaatgac gtgtggatgc gccgccactt cacctcgcgc
      361 cagcttgacg atgtcaattc cttcatactg gtttggtggg gtaattccag cataggccag
      421 catggttggg gcaatatcca cgtgagaaac cggcatggtc agccgttttg gttcggcacc
      481 gggtgcataa acccagaagg gcacaaacac ctgttcaatg tagggcaggc agccgtgttc
      541 gagggcgccg tgttcattaa agccttcgcc atgatcagag gtgaaggcaa tcagcgtgtt
      601 gtttaacagt cctgattcct gtaagtagct gagcaaccga ccaatgtgca ggtccgtgaa
      661 ggtaatttca ctgtcataaa tgtccgggtc cgtttcgcca tagccacatt cggcgtgggc
      721 ttcgtacggc gcgtgtggat ccgtgtagtg aatccaggca aaaaacggcc ctttgtcttt
      781 catccgtttg agcaaatcaa ttgaccggtc ggtcatttcg tccgctttcc agaagcgagc
      841 ttcttcgtgg gtaatttcct gatagctctc catcatgatt tgcttgtatt caaaccagcg
      901 acctttatag agccaggttt tggtcttcgg actggtaata aaagcggttt gatacccctt
      961 gtcagcaaac aatttggcga ctgttggcac actgttgtca aaaatgccgt cgccctgggc
     1021 tgagcgcata attgaagaaa acgaatggcc ggtgttggtg ccgctactgt agccgtgttc
     1081 gcaaaaaatg ctctgtttgg caaatcggtc cagattgggc gttgtgtgcc gcttgtagcc
     1141 ataaatgctc atatggtctg cccgcaacgt gtcaatcgag agaaaaatca cgtttcgcgg
     1201 gccagtcgca gcggttgaaa gcggggttgt gaccgatccg cccaatgccg tggttgcggt
     1261 tgagagcgct gaggtcaatg tttgtttgat agattgggag gcagccgcac cagatccagt
     1321 ctggtctttc ttttcggcgg cggaaaccgg tggcaccggg atcaaatcgt gcgtgtcggt
     1381 tttgaccggt ggcgggatat ttttggcgat gaccgaggtg aagtcttttt gctcgccgtc
     1441 agtgagtgat cgagcaaagt tcacatatcg cttggccacc acgccgcgcc gccagaagag
     1501 cgattttaaa ttctggttcg tctcatactg ctgcaacgca aacactgagg cgcccaccac
     1561 cgccagcacc agcactccaa tcaccgcccc tccaaatcgc gtgacaatga ccagccggta
     1621 caaaatagcc gagagtatca gagaagaggt caaggctgtc agatacattg ggacgtgaaa
     1681 gctcaaatcc cgacgagtga ccataatgtt ggcatcggca aaataactca ccaccagcag
     1741 ccacagcccg gcaagcgcta aaacaggata taaaatctgt gggttgagca gtttggccaa
     1801 ccgatgcgac cattttggag atggggtttc ctgggaattg gggagcagat aggtggtcgc
     1861 cagttcatcc agcacacaaa atagaaaggt caatcccgcc agaatcaacg cggtcgaaat
     1921 cttgcgatgg ccaaacacca gccgaaatgc ccacattttt tcggttgcgc cagcaaacat
     1981 gccatacagc ggatcactga aatacgccgg aaagagaacg gcgagcacgc caaagaaggc
     2041 aaatacggca gccattccaa gcacccgttg ccgccagtgg ccagggagtt caaatttccc
     2101 aattcggttg ggagtttcac ctgaggtgaa ttgaaaccca acgagccgaa gactggacgt
     2161 gagaccaaga aacaaccccc agagcgggcc gccaagggcg gtaaacacaa aatacagcca
     2221 gaagattttg ctttcgcc
//
LOCUS       DAOQDO010001030         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45287_length_2237_cov_1.986251, whole genome shotgun sequence.
ACCESSION   DAOQDO010001030 DAOQDO010000000
VERSION     DAOQDO010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45287_length_2237_cov_1.986251"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKZ53_15475"
     CDS             complement(<1..214)
                     /locus_tag="PKZ53_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41890.1"
                     /translation="MTTENAFARGGRVVFMTACTLICLLAATSGIPIGVLANAEKSNT
                     SKKSSARVLSEEQRIVHLLNRLGYGPR"
     gene            complement(312..1079)
                     /locus_tag="PKZ53_15480"
     CDS             complement(312..1079)
                     /locus_tag="PKZ53_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ41891.1"
                     /translation="MTEILLTIESSIATITLNRPEKFNALAGDMREQLLSAIRTVRDD
                     EAARVLIITGAGKAFCAGGDVLAMYQTQQENQVDRISSWLELGGDIVQSLRHLPKPVI
                     AAVNGVAAGAGCNLALACDVRFASEKASFCQAFVRLGLHPDWGGSFFLPQLIGTARAA
                     ELMFTGDVVDAQTAQTLGLVNRVVSPDELLPQVTEFARRLTTFSAESLAEIKESLYSV
                     TSLAEAVRRERDVQLKLWASESSRARISDFVERRKVK"
     gene            1433..>2237
                     /locus_tag="PKZ53_15485"
     CDS             1433..>2237
                     /locus_tag="PKZ53_15485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ41892.1"
                     /translation="MNTTDPSLSTIEDGPLFNGIDCQTELESRKMNPGFNSLLTIRHL
                     GDMATEEDRQSNTLRSNTVVPSRTQQPEPAPDQYLGELVNNRYLVEKELGRGGLGVVY
                     LARDKHLLSKPVVLKVLLEESIKNEWARTKFSQEIEALARIDHPGIVGVLDAGEMPDG
                     KPYLAMQFVEGKSLRSIIKEGGIGPERVKNLVQQMAKALTAVHEKGIYHRDLKPENIM
                     LQTMDDGEEQIKIIDFGIARVENSELTVDTSASSMLVGTIAYMSPEQRKG"
BASE COUNT          605 a          541 c          548 g          543 t
ORIGIN      
        1 gccgggggcc atagcccagg cgattgagca gatgcacaat tcgttgttct tcagaaagga
       61 cacgagctga tgattttttg gaagtatttg atttttcagc atttgccagt acgccaatcg
      121 ggattcccga agttgccgcg agcaggcaga tcagagtgca ggccgtcatg aagacaacac
      181 gacccccacg ggcaaaggcg ttttcggtag tcataaatcc actccttacc tatttttgca
      241 gactatgcaa ttgtttgttc agttcgcgtt gtttgatgcc ggtgggttga aaaaagtctg
      301 gacaaaatcc gctactttac ttttcttcgc tcgacaaaat cgctgattcg ggcacgtgaa
      361 ctctccgatg cccaaagttt gagctggacg tcacgttcac gacggacggc ttctgccagc
      421 gaagtcactg aatagagtga ctctttgatt tcagccagtg attcagccga aaacgtggtt
      481 aatcggcggg caaattcggt tacctgaggc agtaactcgt caggtgatac cactcgattg
      541 accaatccga gcgtttgggc agtttgggca tctaccacat ccccggtaaa catcagttcc
      601 gccgcacgcg cagttccgat gagttgcggc aggaaaaagc tcccacccca atcaggatgc
      661 aacccaagtc ggacaaatgc ctggcaaaaa ctggcttttt ccgaagcaaa ccgtacatca
      721 caggccagtg ccaggttaca gccagctcca gccgccacac cgttgacagc ggcaatcact
      781 ggtttgggaa ggtgcctcaa cgattgcaca atgtccccgc ccagttccaa ccagcttgaa
      841 atgcgatcca cctgattctc ttgctgggtt tgatacatgg cgaggacatc accaccagca
      901 caaaaggctt tcccagcgcc agttatgatc agaactcgtg ccgcttcatc atcccggaca
      961 gttcggatcg cgctcagtaa ttgctcacgc atgtcgcccg ccagggcatt gaatttctca
     1021 gggcggttca aagtgatggt cgcgattgag gattcgatag tgagaagaat ttcagtcatg
     1081 tggttttgcc attggaaaaa agaaaagaag gttgttcctg atcaaataag ttgctgcagt
     1141 tgggcgacag actgccacct gcctggcaca gtttgagtca atcagggttt cagaaaccag
     1201 acctggattt tcatattaac aacaacttct tcttaatcag tgttttacat attagaaacg
     1261 gtgcacctgg aatctttcgg cacaccaaat gcactcgtat tccttctgtg aaaaaggtga
     1321 gaacctttga atttcagtga ttgcgctcaa gcagccaaac tttgttttga ttcggaagag
     1381 tcaactcaat tcccaattgc ttgatatgca cttgacttca gaagaccaac ctatgaatac
     1441 aacagatcca tcgttgtcca cgattgaaga cgggccactt ttcaatggaa tagattgcca
     1501 aaccgaactg gaatcacgga aaatgaatcc tggatttaat tcccttttaa caattcggca
     1561 tttgggtgat atggccacag aagaagaccg tcagagtaat acacttcggt ccaacaccgt
     1621 tgtcccatcc aggactcagc aaccagaacc tgcaccagat caatatctcg gagagttggt
     1681 caataatcgg tatctggttg aaaaagagct tgggcgtggc gggttgggtg tggtgtatct
     1741 ggcacgtgat aaacacctct tgtccaaacc agtggtcctc aaagtcttgc tcgaagaatc
     1801 catcaaaaat gagtgggctc ggaccaaatt tagccaggaa atcgaagccc tggcccgcat
     1861 tgatcatccc ggtattgtgg gtgtgctcga cgccggcgaa atgccggatg ggaaaccgta
     1921 cctggcaatg caatttgttg aagggaaatc acttcgatca atcattaaag aagggggcat
     1981 tggaccagag cgagtcaaaa acctggtgca gcaaatggcc aaagccctga ctgcggttca
     2041 tgaaaaagga atctatcatc gggatctgaa acccgaaaac attatgcttc agaccatgga
     2101 tgatggcgag gagcaaatca aaattattga ttttggaatt gcacgggttg agaactcaga
     2161 acttacggtt gatacctcag ccagttcgat gctggttgga acgattgcct acatgtcacc
     2221 cgagcaacgc aagggtg
//
LOCUS       DAOQDO010001031         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45347_length_2235_cov_2.966514, whole genome shotgun sequence.
ACCESSION   DAOQDO010001031 DAOQDO010000000
VERSION     DAOQDO010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45347_length_2235_cov_2.966514"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..326)
                     /locus_tag="PKZ53_15490"
     CDS             complement(45..326)
                     /locus_tag="PKZ53_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41893.1"
                     /translation="MEQDKGYILYLDKNKVTIDNIHFTAQHEWRVVGTYEIKRSGQPD
                     IVLSGKFQNTNGLTAELQIVIDGKKQQEVWILQNGGWVTGKHSVAIDEP"
     gene            complement(648..>2235)
                     /locus_tag="PKZ53_15495"
     CDS             complement(648..>2235)
                     /locus_tag="PKZ53_15495"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41894.1"
                     /translation="LRSQLADFLYSSHIIPNPATLSEAQPSTAVTLTEDPNTVTLLAI
                     QECGEVGTATSTVTLDTTAPVLTITSPPDGAITTQTQITVVGTVTHTSPVTVLINGQL
                     ARLSGNRFTALVSLVEGINILTLVAVDSDHHATSLTRQVIRDSTPPTIVIAAPEDGKA
                     TSEAQILVTGEIADATSTLVRVNNQFAPLTGTTFHITVPLTEGVNPLSIVAIDGAQNT
                     TTKVLQIERDSKEPALHVTSPVETIFVTEAAQVTFRGTVTDVTTTTVYVNSEIAPLNG
                     TTFELTFPLREGRNKITVGAVDEAENISAMTFTITRQTIIPLTLTVKFPIDGDITRSP
                     EIMIVGDVTGTGTVQVTANGIPIRVTPYGEVISKQFEISEGNNSLHLVAVDALHNRVE
                     VVRTVFKDSVPPVISDLSPPNRAIITTPTITLEGNVADATGVTVEVNEVEVPVSEGHF
                     IATNIPIAEGENIFWITATDEAGNSVSEVIRLTGKDQPRPTQPVFDQNAPHSEYQRAH
                     FLKSHFTILNGTSGRKDNED"
BASE COUNT          553 a          484 c          525 g          673 t
ORIGIN      
        1 ccaaattcaa tatctcagga accgtatgac aggtatttgc cagtttaagg ctcatcaatt
       61 gcaacggagt gcttccctgt tacccagcct ccattctgta aaatccatac ttcctgttgt
      121 ttctttccat caatcactat ttgtagttct gcggttaatc cattggtatt ctgaaactta
      181 cctgacagta caatatcagg ttgtccgctc cttttgattt cataagtacc gacaactctc
      241 cattcgtgtt gcgcagtaaa atgaatgttg tctatcgtta ccttattttt atctaaataa
      301 agaatatacc ctttatcttg ttccaagttt acatcccaaa cttcaagtct cccactgctt
      361 ggagatcgat attcgctaag aaaactaatt atttttttcc ataaaattgt cttgtgagcc
      421 gatttttttt taaaaaagaa aagggtatat tcttgtatta tttttctcca cctaaaatat
      481 aaaattctcc atcctttcgt ggagtaatat atgttgaggt cctcgccgtc ttataaaaaa
      541 ctacttttcc tgctggtagg tgtttttcaa ataatttggg tccctcgata ccaggagaat
      601 ggattgccag ataagcggtg agcatccaaa tgaatagggt gttggtttca gtcctcattg
      661 tcttttctcc ctgaggtgcc gtttaaaatt gtaaagtgac ttttcaagaa atgggctctc
      721 tggtactcgc tgtgaggtgc attttgatca aaaactggct gtgtgggccg gggttggtct
      781 ttaccagtca atcgaatgac ctcagaaaca gaattaccag cctcatcagt tgcggtaatc
      841 caaaaaatgt tttccccttc agcaatcggg atattggttg caataaagtg cccttcggaa
      901 acaggtacct caacttcatt tacttctacc gtaactcctg tcgcatcagc aacattccct
      961 tcgagtgtga tagttggagt cgtaatgatt gcgcggtttg gcggggaaag atcactgatg
     1021 actggaggca cggaatcttt aaaaacggta cggacaactt caacccgatt gtgtaaggca
     1081 tcaactgcta caaggtggag cgaattattg ccctccgaaa tttcaaattg ttttgagatg
     1141 acctccccgt atggagtaac tcgaattggt atgccattgg ctgtgacctg aaccgttccc
     1201 gtaccagtga cgtctcccac gatcataatc tctggactcc gggtgatgtc accatcaatt
     1261 gggaatttca ctgtcaaggt gagtggaata atcgtctgcc gggtaatcgt aaaggtcatt
     1321 gcgctaatat tctctgcctc atccaccgca ccaacagtta ttttgtttct tccttctcgg
     1381 agaggaaacg ttaactcaaa ggtggtacca ttcaaaggcg caatttcact attcacatag
     1441 acagtcgttg tggttacatc ggtgactgtt cctcggaagg tgacctgtgc cgcctcggtg
     1501 acaaatatgg tttctactgg tgaagtcaca tgaagagctg gctctttgga atcacgttcg
     1561 atctgaagta ctttggtcgt tgtattctgt gcaccgtcaa ttgcgacgat agacagagga
     1621 ttgactcctt cagttaatgg aaccgtgatg tgaaacgtgg taccggttaa aggagcgaac
     1681 tggttattca cccgaaccaa cgtgctggtg gcgtcggcga tttcgccagt gacgaggatt
     1741 tgggcttcag aagtcgcttt accgtcttct ggtgccgcaa tgacaatggt tggtggtgtt
     1801 gagtctcgga tcacctgacg agtcagggac gtagcgtgat ggtcactatc tacggcgacc
     1861 aatgtgagga tatttattcc ttcaaccagt gacacgagag ccgtaaatcg atttcccgaa
     1921 agcctggcaa gctggccatt gatgagaacc gtgacagggc tggtatgggt tacggttcca
     1981 acaacagtga tttgggtttg ggttgtgatg gcgccgtctg gcggggaagt gatcgtcaaa
     2041 actggtgctg tggtgtcgag tgtgactgtg ctcgttgctg ttcccacctc tccacattcc
     2101 tgaatggcta aaagggtaac cgtgtttggg tcttcagtca gtgtaacggc ggtgctgggt
     2161 tgtgcctcgg aaagagttgc cggattggga atgatgtgtg acgaataaag aaaatccgcc
     2221 agttgactcc tcaaa
//
LOCUS       DAOQDO010001032         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45395_length_2234_cov_2.589261, whole genome shotgun sequence.
ACCESSION   DAOQDO010001032 DAOQDO010000000
VERSION     DAOQDO010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45395_length_2234_cov_2.589261"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PKZ53_15500"
     CDS             complement(<1..1461)
                     /locus_tag="PKZ53_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ41895.1"
                     /translation="MHKDSPKSYSSGTSTTPLLGLTIGDLFDQIVDQFPDQEALIVRH
                     QKRRYTYRQFQVVVNECARGLLKLGIQKGDRVGIWSQNNAEWATLQYATAKIGAILVN
                     INPSYRLHELEYALKQSGCRVLVFAPHFKTSNYTQMVCELIPELHASEPGKLDVSTFP
                     HLKILISLEPEPVTGLLCWSDLATLAQAISPEELASRQGQLEFDDPINIQYTSGTTGF
                     PKGATLSHHNILNNGYFVGEGMKFTERDRLVIPVPLYHCFGMVLGNLACLTHGAAAIY
                     PSEGFDPRAVLETVEAERATALHGVPTMFISELEHPDFSNYDLRSLRTGLMAGAPCPV
                     ELMKKVTTLMHMHEFEIAYGMTETSPVSFQTRTDTPPEKRISTVGQILPHVEAKIIDP
                     ETGQVVPIGQPGELCTRGYNVMPGYWNDPETTHLSIDQARWMHTGDLATLDDGGLTAC
                     SMKGLLRILDATALSDAQIDQALLDSRLDPKARKPSV"
     gene            1665..2123
                     /locus_tag="PKZ53_15505"
     CDS             1665..2123
                     /locus_tag="PKZ53_15505"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ41896.1"
                     /translation="MTHSHLQILIRDARPDDAEVLSELFDELGRPTPAATVVERMKTL
                     DGPGNRILVAEYNGAVAGLLTLHQTPNLHAGPDGRITALVVSAQARNLGIATRLVQAA
                     EAICREWKCHRMEVMSAIERVDAHRLYEHLGYRETRKRFRKELEIDRTDR"
BASE COUNT          498 a          577 c          613 g          546 t
ORIGIN      
        1 cacggacggc ttgcgtgctt tcggatcgag acgggaatcg agcagcgcct gatcgatctg
       61 ggcatcggaa agggcggtcg catcgaggat gcggagcaat cccttcattg aacacgcggt
      121 caatccaccg tcgtccagcg tggccagatc tccggtgtgc atccagcggg cctgatcaat
      181 ggaaagatga gttgtttcag gatcattcca gtagccaggc atcacgttgt agccgcgggt
      241 gcagagttcg ccaggctgcc caatcggcac cacctggccc gtctctggat caataatttt
      301 ggcttcaacg tgcggcagga tttgcccaac cgtactgatg cgtttttcag gtggagtgtc
      361 ggttcgagtc tggaagctga ccgggctggt ttccgtcatt ccataggcaa tttcaaactc
      421 gtgcatatgc atgagcgtgg tgactttttt catcagttca accgggcaag gcgcaccagc
      481 catgagtcca gttcggaggc tgcgcaggtc ataattggaa aaatcaggat gctccagctc
      541 actgataaac atcgttggca cgccgtgcaa ggcggtggcc cgttcggctt cgaccgtttc
      601 aagcaccgcc cgtgggtcaa atccttcact cggataaatc gcagcggcgc catgggtcag
      661 gcaagccaga ttcccaagaa ccattccaaa gcaatgatag agtggtactg gaattaccag
      721 ccggtcacgc tcagtgaatt tcattccttc accgacaaaa tagccgttgt tcaaaatatt
      781 gtggtggctg agggttgctc ccttgggaaa gccagttgtg ccgctggtgt attgaatatt
      841 gattgggtca tcaaattcga gttgaccttg acggctggct aactcttctg gactgatcgc
      901 ctgtgccagg gttgccagat ctgaccagca cagcaaaccc gtgaccggtt ctggttccag
      961 gctgatgaga atcttcagat gtggaaacgt gctgacgtcg agcttcccag gttctgaagc
     1021 gtggagttca ggtatcaact cacacaccat ctgggtgtaa tttgaagtct tgaaatgcgg
     1081 ggcaaacacc agcacccgac accccgattg cttgagcgca tattcaagtt cgtgcaaccg
     1141 gtaacttggg ttgatgttga ccagaatcgc cccaattttg gcggtggcat actgcagggt
     1201 cgcccactcg gcattgttct gagaccagat tccaacgcgg tcgcctttct ggatccccag
     1261 ttttaagagt ccgcgggcgc attcgttgac gacgacctgg aattggcgat aggtgtagcg
     1321 tcgcttctgg tggcgaacaa tgagggcttc ctgatcggga aactgatcaa caatctggtc
     1381 aaacaggtct ccaatcgtca ggccgagcaa gggcgtggtg ctggtgccac tggaatagct
     1441 tttaggagaa tctttgtgca tcggaagggg tcctgtgaat acagttcata aaaagccttc
     1501 gctttttacc acagagcgaa cttcagaacc cagaggaaga ctgattgact ggctcaatcg
     1561 gtgggtgttt cttaagattc ggctttactt tttgactggt ttttcctatt accttcaaac
     1621 caatccactt gattgatcct tgcccaccct gacccaaacc agagatgaca cactcacacc
     1681 ttcaaatcct gattcgtgat gcccggccag atgatgctga ggttttatct gagctattcg
     1741 atgaacttgg ccgaccaacc ccagccgcga cagttgttga gcgaatgaag acgctggatg
     1801 ggccgggcaa tcggatcctg gtggccgaat acaatggcgc cgtggctggt cttttgaccc
     1861 tgcaccagac accaaacctc cacgctggcc cggatggtcg cattaccgca ctggtggtca
     1921 gtgcccaggc tcgcaatctg ggaattgcca cccggctggt tcaagccgct gaagccattt
     1981 gccgtgagtg gaagtgccac cggatggaag tcatgagcgc tatcgaacgc gttgatgctc
     2041 atcgcctgta tgaacacctt ggctatcgtg agacccgcaa acggtttcgc aaagaacttg
     2101 aaattgatcg aactgatagg taaagccctg gaattgctgg agttttctca atgctcattg
     2161 ctttctggat cgtgcttgga ctcaatgcac tgtggtttgg tgcggcgttc aggtacttta
     2221 gcctcacacc tgag
//
LOCUS       DAOQDO010001033         2233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45428_length_2233_cov_3.095041, whole genome shotgun sequence.
ACCESSION   DAOQDO010001033 DAOQDO010000000
VERSION     DAOQDO010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45428_length_2233_cov_3.095041"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKZ53_15510"
     CDS             complement(<1..274)
                     /locus_tag="PKZ53_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41897.1"
                     /translation="MKLTPLSLFGLAAGIYLLAMGLPFGLKLAGCEPFFQAHTWLPHF
                     LLKTALVGISLVAMWKTDRSWAKWGFTRPNPHVSWWTVILSGGVIGA"
     gene            complement(274..1164)
                     /locus_tag="PKZ53_15515"
     CDS             complement(274..1164)
                     /locus_tag="PKZ53_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ41898.1"
                     /translation="METDQEKCIALLRDLGLNQLEAEIYTFLLPNEPMTAYRVGKAIG
                     RPTANVYKSIENLARFGAVLVEEGTNRVCRAVPVEEFIRHTQNQFLEKTQAAQQVLAL
                     LQRPTFDERVYRIESVADVLKRSRDMLARCQRIAVLDAFPHSLEALLPSLKEAVARGV
                     EVIVEAYMPVEIEGADVVVVPDGQNSLNAWQSEQLNLVIDGKEHLLALLSQDLKDVYQ
                     AVWSGSVYLSCLHHSGRMHEISLLRLIHLLNKNQSPEALRDVLNTNRFFRSDDIPGHR
                     ELLRRFGKVPPVATNREEAN"
     gene            complement(1283..>2233)
                     /locus_tag="PKZ53_15520"
     CDS             complement(1283..>2233)
                     /locus_tag="PKZ53_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ41899.1"
                     /translation="SFAGNTPASPQPQITLTIRILPDAATRELELASGSLDFLLDADL
                     QPSQLEQWKSNPDWNVTVFPGGGLVYLSLNTEHPLLKSVKARQALALAINRPEIINRV
                     LGGNAEPALSPLPPKHWALSPNLMRFDFERQQATRLWQEANGTGPAKLTLLVTADANQ
                     RRIGGILQEHWRQIGVQVEIQSVETALYFERLRYGDYAIALAKLTGGNQFVTIFKGAF
                     HSRAIHVRGTTDTGELNYARYRNPDVDALIDQAQQSPFSMDQATEASKYLAIQKKIVE
                     EAPWIFLWHPSHVLVARTSLDGFEFPPGGSGEVYARVHYR"
BASE COUNT          517 a          582 c          614 g          520 t
ORIGIN      
        1 ccgcaccaat cacgcctccg ctcaaaataa ccgtccacca ggaaacgtgt gggttgggtc
       61 tggtaaatcc ccatttggcc caggagcgat ctgttttcca catggcgacc agcgaaattc
      121 caaccagggc cgttttgagc aaaaagtgcg gcagccatgt gtgagcctga aaaaatggct
      181 cgcacccggc cagtttcaac ccaaaaggaa gacccattgc cagcagatag atgcctgcag
      241 ccagtccaaa caggctcaag ggcgtcaatt tcatcagttt gcctcctcgc ggttggtggc
      301 gacaggtggg actttgccaa atcggcgcag taattcacga tgtccaggta tgtcatcact
      361 tcggaaaaaa cgattggtgt tcagcacatc ccgaagtgcc tctggtgatt gatttttatt
      421 gaggaggtgg atcagtcgca ggagggaaat ttcgtgcatc cgtccgctgt ggtggaggca
      481 ggacaggtag acactgccac tccacactgc ctgatacacg tctttcaaat cctggctcag
      541 aagagcaagc aggtgttctt tcccatcaat gaccagattg agttgttcgc tttgccaggc
      601 attgagcgaa ttctggccgt cggggacaac gaccacatcg gcgccctcga tctcaactgg
      661 catataagct tcaacaatta cttcgactcc gcgcgcaacg gcttctttca aactgggcag
      721 cagcgcctca agcgagtgcg gaaacgcatc aagcaccgct atccgctgac agcgggccag
      781 catgtcgcgg ctacgcttca ggacgtcagc cactgattca atgcggtaca cccgttcatc
      841 aaaggtcggt cgctgcaaca acgcaagtac ctgttgggcc gcctgtgttt tttccaggaa
      901 ctggttttgg gtatggcgaa taaattcctc aaccgggacc gcgcgacaaa cccggttggt
      961 gccttcttcg accagcacgg cgccgaaccg ggccagattc tcaattgatt tatagacatt
     1021 ggctgttggg cgaccgatgg ccttgccgac ccggtatgcc gtcatcggtt cgtttgggag
     1081 gaggaaggta taaatttccg cttccaactg attgagtcca aggtcgcgca gtaaggcgat
     1141 acatttttcc tggtcagttt ccatgatggt gtcctgagtg ttttgatttt tcgtagtata
     1201 tttttatgct acgaaaaatc aagagaaaaa tattccactt gaatcaggcc acatttttct
     1261 caagccctca gattgtgaat agttaccgat agtgaaccct ggcatagact tcaccactgc
     1321 caccaggtgg gaattcaaat ccatcaagtg aagtccgggc caccaggacg tggcttgggt
     1381 gccagaggaa aatccaggga gcttcctcga caattttttt ctgaatggcc agatattttg
     1441 acgcttcggt ggcctgatcc atcgaaaacg gcgattgctg tgcctgatca atcagggcgt
     1501 caacgtcggg gttgcgatac cgggcatagt tgagttcacc cgtgtctgtg gttccacgaa
     1561 cgtgaatcgc ccgcgaatga aacgcccctt tgaaaatcgt gacaaactga ttgccaccgg
     1621 tcaatttcgc cagggcaatc gcataatcgc cataccgcag ccgttcaaaa tagagcgccg
     1681 tttcaaccga ttggatttcg acctggactc cgatttgccg ccagtgttcc tgcaaaattc
     1741 caccaattcg gcgttgattg gcatcagcgg tgaccagcaa cgtgagtttg gcagggccgg
     1801 tgccattggc ttcctgccag agtcgggttg cttgctgtcg ctcaaagtca aaacgcatca
     1861 ggttgggtga gagcgcccag tgtttgggag gcaggggcga cagggctggt tcggcatttc
     1921 cgcccagaac acggttgatg atttcaggac gattgatggc cagcgccagg gcctgacgcg
     1981 ccttcaccga tttcaggagc ggatgttcag tgttgagcga gagatagacc agaccaccgc
     2041 cggggaacac cgtcacgttc cagtctggat tggacttcca ttgttcaagc tggctcggct
     2101 gcaagtcggc atcaagcaga aagtcgaggc tgccgctggc cagttcgagt tcgcgggtgg
     2161 cggcatcggg caaaatccga atagtcagtg tgatttgagg ttggggtgaa gcaggggtgt
     2221 taccagcaaa cga
//
LOCUS       DAOQDO010001034         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45466_length_2232_cov_3.255857, whole genome shotgun sequence.
ACCESSION   DAOQDO010001034 DAOQDO010000000
VERSION     DAOQDO010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45466_length_2232_cov_3.255857"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKZ53_15525"
     CDS             <1..216
                     /locus_tag="PKZ53_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ41900.1"
                     /translation="NETGGRVFPGSTVDELIAGFGQLTTELRSQYKLSYYSNNDAQDG
                     KFRKIDLRVKRPDLKVRTREGYFAPKN"
     gene            complement(253..702)
                     /locus_tag="PKZ53_15530"
     CDS             complement(253..702)
                     /locus_tag="PKZ53_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNJ41901.1"
                     /translation="MEPSPQISSSLTFPVLLYDGVCGFCNHSVQFILRFDRRGTLQFA
                     PLQSEFAQTLLTRYPHLKSIDSVMYVAPAHNGQAEQVYVRSSAALKIAAYLGGWWNIF
                     RVGSLIPRGLRDLVYDLFAKHRYRWFGKHDACLLPSAEVRSRFIGFS"
     gene            complement(804..1055)
                     /locus_tag="PKZ53_15535"
     CDS             complement(804..1055)
                     /locus_tag="PKZ53_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41902.1"
                     /translation="MSDIDVTFRNENNGKEVEVTVSDDMTSDAILRNLAEAGLIPSVP
                     GTTLVYRNMQLRPGQTLAQAGVVSGEAVSIFLNTRGGRY"
     gene            complement(1123..1440)
                     /locus_tag="PKZ53_15540"
     CDS             complement(1123..1440)
                     /locus_tag="PKZ53_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41903.1"
                     /translation="MDFLALGALFFLVVVAFSVGLEIQDRYQEGSDGLRGFRLLPRRN
                     YYDLHDDAYGFSLPPPEASNDASGTFLVKREDRPSRCEVCHQVDMYHLESGHCQRCNH
                     TTM"
     gene            complement(1657..>2232)
                     /locus_tag="PKZ53_15545"
     CDS             complement(1657..>2232)
                     /locus_tag="PKZ53_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41904.1"
                     /translation="HQERVECGVRLAYNGFTHGFHRIQLPENPDCLAHGHLSTIVESP
                     AQAKTTTLGDFLGWLETEFGTGVHIDLGRRFIEGIRCRSCHQWIELNRPAHRVFDDEM
                     YCPDCRLGAKHQEKPVLAAPTDTSTYLKAATRISRHHLGDSPLPSPTLDLTLWDLGIP
                     QLHILSVTDAQGRVHSVELTGDIAELLADWD"
BASE COUNT          592 a          534 c          571 g          535 t
ORIGIN      
        1 aatgaaactg gggggcgggt atttccaggt tcaaccgtag acgaattgat tgccggcttt
       61 ggacaactga ccaccgaact gcggtcgcaa tacaagcttt cctattattc taacaatgat
      121 gctcaagatg ggaagtttcg aaagattgat ctgcgtgtga agcggcccga tttaaaagtc
      181 agaacccgtg aaggctactt tgctccaaaa aattaagtgc cctggctgat acggcttctc
      241 aatcctgggg ggtcaggaaa atccaataaa ccggcttctc acctcagccg acggaagcag
      301 gcaggcatcg tgtttaccaa accatcgata gcggtgtttg gcaaataaat catagaccag
      361 gtcccgcaat cccctgggga tcagacttcc aactcgaaaa atattccacc atccgcccag
      421 gtaggctgca attttcagcg cggcacttga gcgaacataa acctgttctg cctgtccatt
      481 gtgtgccggg gcgacataca tcaccgaatc aatacttttc aagtgtggat agcgggtcag
      541 gagtgtctgg gcaaactcgc tctgcaacgg ggcaaactgt aaggtgcccc gtcggtcaaa
      601 tcgaaggata aattgaacac tgtgattgca gaacccgcaa actccatcat agagcaagac
      661 cggaaaggtc agactggatg aaatttgtgg tgagggttcc atagttgatg caggaaaagc
      721 agatgggtga atgaggaatg ataaaatgga taactcattg atcttcaatg agatagtctc
      781 cttataactc atcattcata atttcaatac ctgccaccac gagtgttgag aaaaatactc
      841 actgcttcac ctgacacaac cccggcttga gccagggttt gtcctgggcg gagttgcatg
      901 ttgcgataga ccagcgtggt accaggtaca ctgggaatta atccagcttc agccagattg
      961 cgcagaatgg catctgaggt catatcatct gagaccgtga cttcgacctc tttcccgtta
     1021 ttttcattcc gaaatgtgac atcaatatct gacattgcgc ctttagccgg gaaatggctt
     1081 tcctcctctc aagaagttag caaaaacagg attgtgtgca atttacattg tggtgtggtt
     1141 acaccgctga caatgacctg attcgaggtg gtacatatca acctgatggc atacctcaca
     1201 tctggatgga cgatcctctc gtttgaccag aaacgttccc gaagcatcat ttgaagcctc
     1261 cgggggaggt aacgagaagc cataggcatc atcgtgcagg tcataatagt ttcgtcgtgg
     1321 aagtaatcgg aaccctcgca gcccatcact cccttcctga taccggtctt ggatttcaag
     1381 cccgaccgaa aaggccacca caactaagaa aaagagtgct ccaagggcta aaaaatccat
     1441 gtatgacctc cagtttggaa ggttgaagca tagccgagaa gcttctcagt gagaagacat
     1501 tcagcccgcg ttcgctgact ttttgtaaat caagctgtgg gttcggagca accctgaata
     1561 attgtgattc ggtaaacgtg atacgggtca attgcagtct accctggatc agcttttcag
     1621 acgagtccta atttcaggct gtgggcccaa tgtttatcaa tcccaatcag ccaggagttc
     1681 tgcgatgtcc ccggttaatt caaccgaatg aacccgaccc tgggcatctg tcaccgaaag
     1741 gatatgcaat tgggggattc caagatccca aagtgtgaga tccagggttg gtgagggaag
     1801 cgggctgtca cccaggtggt ggcgtgagat tcgggtggcg gctttcaaat aggtgctggt
     1861 gtcagttgga gctgccagaa ccggtttttc ctggtgtttg gcgccaagac ggcagtcagg
     1921 gcaatacatt tcatcatcaa aaactcgatg cgccgggcgg ttgagttcga tccactggtg
     1981 acaggaccga caccggatac cttcgataaa tcgtcgccca agatcaatgt gaactccggt
     2041 cccaaactcg gtttcaagcc agcctagaaa atcaccgagc gtcgtggttt tagcttgggc
     2101 cggtgattca acaattgtgg acaggtgacc gtgagccaga cagtcaggat tttcagggag
     2161 ttgaatgcga tgaaatccgt gggtgaagcc attgtaagcc agccggacac cgcattcgac
     2221 ccgttcctga tg
//
LOCUS       DAOQDO010001035         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45486_length_2232_cov_2.350023, whole genome shotgun sequence.
ACCESSION   DAOQDO010001035 DAOQDO010000000
VERSION     DAOQDO010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45486_length_2232_cov_2.350023"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..977)
                     /gene="prfB"
                     /locus_tag="PKZ53_15550"
                     /pseudo
     CDS             complement(1..977)
                     /gene="prfB"
                     /locus_tag="PKZ53_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148264119.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(1263..2012)
                     /locus_tag="PKZ53_15555"
     CDS             complement(1263..2012)
                     /locus_tag="PKZ53_15555"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNJ41905.1"
                     /translation="MIYSARKLLTLWAWIGLVVLTSVACNQSGNHGFTMKGPIDLTKI
                     PKTFQAKMQVQYEVGIQTIRPDLIAIARKNGNVRAEVTRNGRKVIQILNEGKKKLYLL
                     SPTEKKYFELPYSEDSTTQFKSQVSPFEEIYTDPNTKVEDGGVETLLGFDCQKVRVIR
                     EKNGGKQTVTLWCAKKLQNFPIRIAGDNPGKKGTYSFVELSFDVPDSLFEPPADYTLE
                     AAPAVPQPETPAPNPAELSGPPPAKPEAEKK"
BASE COUNT          586 a          541 c          475 g          630 t
ORIGIN      
        1 aaacgtagtt gcgtatctgc gagccccatt caattttcat tttggtgccc tctattttgt
       61 cccgctcctc atagcgcttc tccaactctt tttcgtaaag tttggattta agcatttgca
      121 aagcgcggtc gcggttatcg tgctgcgaac gcgtaacctg gcaggtaact actatattgg
      181 ttggtatatg gcgtaaacgc acggctgttt ccaccttgtt tacgttttgc ccaccggcgc
      241 cgctactgcg gaaagtatcc cactccaatt cagatgcagg aatattgatt tcaatcgtat
      301 cgtcaataac cgggtaaaca aaaaccgatg caaaggatgt ttgcctttta ccctgcgcat
      361 taaaaggaga aatacgaacc aaccgatgga cacccgattc acacgagagc aacccatagg
      421 catattcgcc ttcaaccgta aaggttgccg aggtaagtcc agcttcttta ccaggttgca
      481 gatccaccac ttcgacctta aaaccccgcc gctcacacca acgcacatac atccgcaaga
      541 gcatttccgc ccaatcttgg gcatcggttc cgccagcgcc ggattgaatg cgaacaatgg
      601 cattattggc gtcattggga ccagacatca gcattttggc ttcgacagct tcgacatcct
      661 gggtcaactt tgccagcgtc gccgaaagct cggtgtacga actctcatct tcggcggcaa
      721 attcaaagag cacttccaca tcacccaggg ttcgatcaaa gcgttcagcc tgacggacgg
      781 cttcttcagc tcgactgcgc tgcttcagga ttttttgggc ctgctcctga tcgttccaga
      841 aatctggagc gctgatctgc gattcaagtt tgtcaaggat cagacgttta ctatccgggt
      901 caaagaaacc tccgcaaatg ggagatccgg tcttttaagt cagaaaaagt tcgtgaaagc
      961 tcatcgagct caagcataag tcctattcac ttttgatttg ataaaaaata gagaagacca
     1021 accgtagcaa agaatatact ttaaatacaa gcgcctacga ctcgaatttt aaaggatttg
     1081 ccaccgcttg cccaaccaac cgggaatgaa gaaaccagta gaatgaagaa tgaagagtga
     1141 agaatgaaga aattttttgg tttctcgttc ctgattcttt ttaattttaa actctcagtc
     1201 ccaaccattt agctctgggt ttgatttctt cattcttcat tctcaattct tcattcaagg
     1261 ggtcatttct tctcagcttc tggtttggcg ggtggaggtc cagaaagttc agccggattt
     1321 ggggccggag tttcgggctg tggtactgcg ggtgcagctt caagcgtgta atcggctggt
     1381 ggctcaaaca atgaatccgg cacatcaaaa ctcagctcga caaatgaata tgtgcctttt
     1441 ttgccagggt tatcaccagc aattcgaatc gggaaattct gtagtttctt ggcacaccag
     1501 agggtaaccg tttgtttccc cccgttcttt tcccgaataa cccgaacttt ctggcaatca
     1561 aagccaagta aagtttcaac tccaccatct tcaacttttg tgtttgggtc agtatagatt
     1621 tcctcaaacg gagagacctg tgatttaaac tgtgtggttg aatcttcact atacggcaat
     1681 tcaaagtatt tcttctcagt tgggctgagc agatacagct tctttttccc ttcatttaaa
     1741 atttgaatca cctttctccc gtttcgcgtg acttcagccc gaacattgcc atttttgcgg
     1801 gcaatcgcaa tcaaatcagg tcgaatggtt tggatcccaa cttcatactg aacctgcatt
     1861 tttgcctgga aggttttggg aattttggtc agatcaatcg gccccttcat ggtgaagcca
     1921 tggttcccac tctggttaca ggccacactg gtgagcacaa caaggccaat ccacgcccac
     1981 aaggtgagca gttttcgtgc agaataaatc atgtttatca gtcctaaatg ctttcattta
     2041 aataaattca caaagaaacg aagcggcaat cagaatgaag aattgagaat gaagaatgaa
     2101 gaatgaagaa tttggttctt ggttcttcgc tcctggaaag tattgcttcc taagcaccag
     2161 gtaccactca ctaaagggtt tcttcattct caattcttca caggacttac gtagagctag
     2221 taactacttt aa
//
LOCUS       DAOQDO010001036         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45507_length_2231_cov_3.119945, whole genome shotgun sequence.
ACCESSION   DAOQDO010001036 DAOQDO010000000
VERSION     DAOQDO010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45507_length_2231_cov_3.119945"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKZ53_15560"
     CDS             complement(<1..309)
                     /locus_tag="PKZ53_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41906.1"
                     /translation="MPHSSKLAAWLRHNLPQTLIINSIIAAIAVLLAGRLFSRPPKQT
                     LQIAVVSLVYSQIIGTLISVSIGLGWVEFKSYHKPWNWIFRILFLLILAAMGTMLANG
                     I"
     gene            complement(458..1954)
                     /locus_tag="PKZ53_15565"
     CDS             complement(458..1954)
                     /locus_tag="PKZ53_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNJ41907.1"
                     /translation="MTRREFAKLLAGASLLSLVDERILAGLDQNRDRLSWRAFQNPSA
                     EGTWKLTKIEGKIPRDLQGTLYRVAPGQKEKYGVKLRHLFDGDAYVAGYQIRDGKVEL
                     KTRFVETPQRMAEVKAGKMLYNEFGTQAPLDPTGPPRGGANQPSVNIIAWDGRLLGLS
                     EGGHPSAIDPNDFGFQGYWDFHGTLPKNVPFTAHPKFDPKTGEGYGFGVKTGMKSALM
                     VYHMLKDGTLKLLFEYPLTSYFMIHDMMISDNFIVFVIPPVTVDLKTMFSGKVSLAEC
                     LRFLETTPTKIVVMRRDGTGTPVTIEQPSNMVFHHGNAFERDGKLVIDSILSANGDIL
                     KRLASLDQDIVSGKSSERLTRLVLDPVKGSVESRTELTELVEFPRFNIHQTGKDARYL
                     YCAQTESNQDEAPFTRLMKFDLHRSKETVAKTKASQVYGEPVFVPHPTGKAEDDGWVL
                     IQGYDGPQNQTFLEIRQAQTLDLAARVWTGQHFPLGFHGNFEFGMFRK"
BASE COUNT          574 a          557 c          554 g          546 t
ORIGIN      
        1 gataccattg gccagcattg tccccatggc agccaggatc agcagaaaca atattcgaaa
       61 gatccagttc cagggtttgt ggtacgactt aaactccacc catcccaggc caatcgaaac
      121 cgaaatcaac gtgccgatga tttgagaata caccaggctg accacggcaa tttggagggt
      181 ttgcttcggc ggacgcgaaa acaacctccc ggccagcaac accgcaatgg ccgcaatgat
      241 cgagttaata atcagcgttt gcgggaggtt atgacgcaac cacgccgcca gtttggagga
      301 gtgaggcata gattggggag gcagggttca ggggtcaggg gtcaggggtc aggggtcagg
      361 taaaaaccaa aggacataaa ggacataaag gacataaagg acataaaggg atactatttt
      421 gaaaaccctg aaccctgaac cctgaaccct gattttccta ttttcgaaac attccaaatt
      481 caaagttgcc gtggaaccca agcgggaaat gctggccggt ccagactctg gcggccagat
      541 cgagggtttg ggcctgacga atttcaagga acgtctgatt ttgaggcccg tcatagcctt
      601 gaatcaacac ccatccgtca tcttcagctt ttccagttgg atggggaaca aagaccggct
      661 caccatacac ctggctggct ttggttttgg ccaccgtctc tttggaccga tgcaagtcaa
      721 atttcatcaa ccgggtaaag ggagcttcat cctggttcga ttcggtctgg gcacaataga
      781 gatagcgggc atccttgccg gtttggtgaa tattaaagcg tgggaattca accagttccg
      841 tcaattcggt tcgactttcg accgaacctt tgactggatc aagcaccagc cgggtcaggc
      901 gctcactgct ttttccactc acaatatcct gatcgagcga agccagccgt tttagaatgt
      961 ctccgttggc tgaaagaatg gaatcaatca ccagtttccc atctcgttca aacgcattgc
     1021 cgtggtgaaa aaccatattt gagggctgtt caatcgtcac gggcgttcca gtaccatccc
     1081 gacgcatgac aacaattttg gttggcgtgg tttcaagaaa ccggaggcac tcggccaggc
     1141 tgacttttcc agagaacatg gttttgagat caactgtcac cggcgggatt acaaacacga
     1201 tgaaattgtc ggaaatcatc atgtcatgaa tcataaagta ggacgtcaac ggatactcaa
     1261 acagcaactt gagcgtgcca tccttgagca tgtgatagac catcaaggct gatttcatac
     1321 cagttttgac gccaaaccca tagccttccc cagtttttgg gtcaaatttg ggatgagccg
     1381 taaacggcac gtttttgggt aaggtcccgt gaaaatccca gtatccctgg aatccaaagt
     1441 catttgggtc aatcgcactc gggtgaccgc cttcggaaag ccccagcagg cggccatccc
     1501 aggcaatgat gttcacgctg ggctggttgg cgccacctcg gggtgggcca gttgggtcca
     1561 gcggcgcctg ggtgccaaac tcgttataga gcattttccc ggctttgact tcggccattc
     1621 gctgcggcgt ctcaacaaat cgggttttga gttcaacctt cccgtcccga atctggtatc
     1681 cggccacata ggcgtctccg tcaaagagat gacgcagttt gacgccgtat ttttcctttt
     1741 gacccggtgc gactcgataa agtgttccct gcagatcccg aggaatcttt ccttcaattt
     1801 tggttaattt ccaggtgcct tcggcgcttg gattttgaaa agcccgccac gacaaccggt
     1861 cccgattctg gtcaagacca gccaggatgc gttcgtcaac cagtgataat aagctggcac
     1921 cggccagtaa tttggcaaat tcacgtcgtg tcatacctgt ctccaaaaaa gtgaatgaag
     1981 aattgagaat gaagaatgaa gaatgaatct ggcacctggt gcttatacca ttttggaatg
     2041 aaactatcgt attagtaaaa ggctaaatta ttgggaaaat tggattttcc tgacccatgc
     2101 ccccgatacc ctgaccccaa aatggtatta gtgcctggga accaatactt tctaagcacc
     2161 aagcaccaag caccaggtct tcattttaca ttgattgttg tcagcaagtc tttggcctgg
     2221 gtttcatatt t
//
LOCUS       DAOQDO010001037         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45538_length_2230_cov_3.390805, whole genome shotgun sequence.
ACCESSION   DAOQDO010001037 DAOQDO010000000
VERSION     DAOQDO010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45538_length_2230_cov_3.390805"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..763)
                     /locus_tag="PKZ53_15570"
     CDS             complement(131..763)
                     /locus_tag="PKZ53_15570"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNJ41908.1"
                     /translation="MSTDTALAQYQALLHWIAQTTSDLTSQFPTEITCHRGCTGCCQQ
                     HLSVFEVEAENLRQFLKLLPEAVRHKLSHQARATLDREARFEAEHIAPDTEFPPDPEQ
                     AIPCPALIDGACAVYPARPVICRTHGFPLLYVDEERDEALLDVCPLNFTEAEDTLNSL
                     IETEVIPMSDINEHLVRANLAFLQNTTGGTANALNRRSIAEILLSVVSSQ"
     gene            1086..2075
                     /locus_tag="PKZ53_15575"
     CDS             1086..2075
                     /locus_tag="PKZ53_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ41909.1"
                     /translation="MATSTSTLRVALIGTGRVGRIRARELNLRNDVRLVAVASHSLTR
                     AQELAAPYQAEATTDWETLVKRPDVDVVMVCTLNQQHAPICEAALQARKHTSVDYPVA
                     LSLAETQRLIDLARSQSVVFHVEHIELLAPWFQALVNNLPKVGQPLAVLWGDLSARKP
                     SLDDWTFNVRSGFSLFAGASVLSRLIRLAGPAQWIDASENLVGTTDDGFFQSRFTTAH
                     IGFANGVIGQIADAVGLAVTGVSHALTIIGTQGMLVAKNRQQVKLAIGTEVIDILPMA
                     HHLSHFAQDVTSFVEAIRFGKPTYVSLDHVLQVMRLADAAERSCREGKRIVLN"
BASE COUNT          518 a          531 c          627 g          554 t
ORIGIN      
        1 actccgtgcc gacattgaaa cggcgaccgg atgtggcgac cctggaacgg ctgacctctt
       61 tacccggatt gtgcaggttc atgaaaaaga tgcatggttt attgccgaac accgcaaacg
      121 ggatcggtga ctactgacta ctgactactg acaaaagaat ttccgcaatc gaccggcggt
      181 tcagcgcatt ggctgtgccg ccggttgtgt tttggagaaa ggccagattg gcccggacga
      241 gatgttcgtt gatatcgctc atcggaatca cttcagtttc gatgagtgag ttcagtgtat
      301 cttcagcttc ggtgaaattg agcggacaaa catcaagcag ggcttcatcc cgttcttcat
      361 ccacataaag cagtggaaat ccatgggtac gacaaatcac cgggcgcgcc gggtagaccg
      421 cacacgcacc atcaattaat gccggacagg ggatagcctg ttccgggtcg ggagggaact
      481 ccgtatcagg tgcgatatgt tcagcttcaa atcgggcttc gcgatcaagc gtggcgcgtg
      541 cctggtgaga tagcttgtgg cggactgctt ctggaaggag tttgagaaac tgacgcaggt
      601 tttctgcttc gacttcaaac actgatagat gttgttgaca gcacccggtg cacccccgat
      661 ggcaggtgat ttcagttgga aactgactgg tcaaatcaga tgtggtttgg gcaatccaat
      721 gcaggagggc ttgatactgg gccagggcgg tgtcggttga cataagtacc tctgaaaaaa
      781 aatgaagcgc cgtacgagag ccgggacaga tccacagcct ttcaggtaag tatttcctga
      841 gtatttgttt tgagaagtct cagaaaccat tgaagaattt ggatttattt tctgtgtatt
      901 gcgtgtattc cgtggtttct ttatctgaaa ttctatattt tcagccgaag cgcccggttt
      961 cttcaattcc aaatcgcatg ttcgagcctg caatggtata aattgggtct cacggcgata
     1021 ccaatgcttc ctcaacgctt acctttgctt ccatcttacc tttgattttc actgacgagg
     1081 tttttgtggc gacttcaact tcaacactgc gtgtggcact cattgggacc gggcgtgtgg
     1141 gacgcatccg ggcccgcgaa ctcaacctgc gcaatgatgt tcgattggtt gcggtggcgt
     1201 cacattcctt gacccgggct caggaactgg ccgcacctta tcaggctgaa gccaccactg
     1261 actgggaaac actggtcaaa cggccagatg ttgatgtcgt gatggtctgt accttaaatc
     1321 aacagcatgc acccatttgt gaagcggcac tccaggcccg caaacatacg tcggtggatt
     1381 acccggttgc tctctcgttg gctgaaaccc agcgcttgat tgatctggcc agaagccaat
     1441 ccgtagtgtt ccacgtggaa catattgaac tgctcgcccc ctggtttcag gcattggtca
     1501 ataatctgcc gaaagttggg caaccgctgg ccgttttatg gggtgacctg agtgcgcgca
     1561 agccgtcgtt ggatgactgg acatttaatg tcaggtccgg tttttcgttg tttgctggtg
     1621 cttcggtgct cagccggttg atccgactgg ctgggccggc ccagtggatt gacgcttctg
     1681 aaaacctggt tggcaccacg gatgacggtt tttttcagag tcgttttact acagcgcaca
     1741 ttggctttgc taacggcgtc atcgggcaga ttgccgatgc cgtcggttta gccgtgacag
     1801 gtgtttcaca cgcgctaacc atcattggaa ctcaagggat gctggttgcc aagaatcgcc
     1861 agcaggtcaa actggccatc ggtactgaag tgattgatat tctccccatg gcccatcacc
     1921 tgagccactt tgcccaggat gtgacctcct ttgttgaagc cattcgattt ggaaagccga
     1981 cgtatgtctc gctcgaccat gtcctgcagg ttatgcgact ggcagatgcc gccgaacgat
     2041 cctgccgcga aggcaaacgc attgtgctga attgacgaaa gaattgagaa tcaaaaacag
     2101 ggctgagggc tgaagaaaat gggctgaaga agacgggctg aagaaagtgg gctgaagaag
     2161 acgggctgaa gaaagtgggc tgaagaagac gggctgaaga aagtgggctg aagaagacgg
     2221 gctgaagaaa
//
LOCUS       DAOQDO010001038         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45556_length_2230_cov_2.480920, whole genome shotgun sequence.
ACCESSION   DAOQDO010001038 DAOQDO010000000
VERSION     DAOQDO010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45556_length_2230_cov_2.480920"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PKZ53_15580"
     CDS             <1..1164
                     /locus_tag="PKZ53_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41910.1"
                     /translation="YFSAGILASFGGAVGFLALRMPVGQALAGALGNWMYLGGNNLLG
                     DGFYKFVTGLDAPVAHTLRMLWLCFMFGSFLLVILGIQSATREIQNQRLLVCVGVGLG
                     VMFVALVPNLVAWQEAGRILPVTTATIFLILLKVCWKHRAERTQLETWMPLTIWAAFS
                     LVLLAKMIFHVRVYHYGFVLAMPATLLLVAVCVEWLPKLARERVGNGDVVQAAVIGLL
                     VAGCLAHLDWSNEFYQHKTYTVGSHGDTMVTFPPEIEPMGLVLNTTAKYLESAAPPNA
                     TVAVMPHGVTLNYLLRRPTSTPFVSYYLFDLKTYGGEAATLERVKQHPPDFIVFLHTG
                     VPDFDVTYFGATPQFGQILSTWIQENYGEVKQFGARPFVDQNPGVVILKRKIS"
     gene            complement(1285..>2230)
                     /locus_tag="PKZ53_15585"
     CDS             complement(1285..>2230)
                     /locus_tag="PKZ53_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNJ41911.1"
                     /translation="LNGTQAMAAVGGLALSKLEKLADLADVIGALSLDALRGTLVAFD
                     PRIQSVRAHAGQGQVAARFRQLLAGSLIRESHKDCRRVQDAYSLRCIPQVHGAVRDVL
                     AHARQVLEIEFNSATDNPLVFPDTGEVISGGNFHGEPVAFALDYTAMAASELGSLSER
                     RLERLVNPDLSEHPAFLAKHPGLNSGLMIVQVAAVALCAENKILAHPASVDSLPTSAN
                     KEDHVSMGMTSAVKLRSIVENVESILAMELLGAAQAIDFLDPLKTSPILQKVHGFVRQ
                     HVPFIEMDVYLHPLITQLKTNLPGIHQLVEADLLKKMG"
BASE COUNT          494 a          547 c          631 g          558 t
ORIGIN      
        1 tattttagtg ctggaatact ggccagtttt ggtggggccg ttggatttct cgcactgcga
       61 atgccggttg gtcaggccct ggccggagca cttgggaact ggatgtatct tggcggaaat
      121 aatttgctcg gagatggttt ctacaaattt gtgaccgggc ttgatgcgcc agtggctcat
      181 accttacgca tgctctggct gtgttttatg ttcggtagct ttttactggt gattttgggg
      241 attcaatcgg cgactcgtga gattcaaaat cagcgattgc tggtctgtgt tggggttggg
      301 cttggggtga tgttcgtggc cttggtgcca aatctggttg cctggcagga agccgggcga
      361 atcttgccag taaccacagc gacgatattt ttgattttgc tgaaagtatg ttggaaacac
      421 cgggctgaac gaacccagct tgagacctgg atgccactca cgatctgggc tgctttttca
      481 ctcgtgttgc tggccaaaat gatctttcac gtgcgtgtct atcattatgg gttcgtgctg
      541 gcgatgccag ccactctgtt gctggttgct gtgtgtgttg aatggctgcc caaactggcc
      601 agagagagag ttggcaacgg ggatgtggtt caggcggcag tgattggctt actcgtggct
      661 ggatgtctgg cgcatctgga ctggtccaat gagttttatc agcataagac ctacacagtc
      721 ggaagccatg gcgacacgat ggtgactttc ccgcctgaaa ttgagccgat ggggttggtg
      781 ctcaacacaa cggcaaaata cctggaaagt gctgctccgc caaatgcaac agtggctgtg
      841 atgccgcatg gtgtgacgct caactacctg cttcgccggc caacttcgac cccatttgtt
      901 tcttattact tatttgacct caagacctat ggtggagaag ccgccacgct tgagcgagta
      961 aagcagcatc cccctgattt tattgtattt ctccacactg gtgtccctga ttttgatgtg
     1021 acgtactttg gggccacacc acaatttggc cagattcttt caacctggat ccaggaaaac
     1081 tatggtgaag tcaaacaatt tggagcccgt ccctttgtcg atcaaaaccc tggtgtcgtg
     1141 attttaaaga ggaaaataag ctgagccggc acggggttga aaattcgcaa gctcagggca
     1201 atcaaaggga tgagggatga aggatgaggg atgaaataaa actaattctt cagcccgatg
     1261 tcttcagccc caatcccgtc ttcttcagcc catcttcttc agcaaatcgg cttcaaccag
     1321 ttgatgaatg cctggcaaat ttgtcttcaa ctgggtaatc agtgggtgca ggtacacgtc
     1381 catttcgata aatgggacgt gctggcgcac aaacccatgg actttttgga ggatcgggct
     1441 ggttttcagc gggtcgagga aatcaattgc ctgggcggcg ccaagcagtt ccatggccag
     1501 aatggattcg acgttttcga caatcgagcg caatttgacg gcgctggtca tgcccattga
     1561 aacgtgatct tctttgttgg ccgaagtcgg cagggaatca accgacgccg gatgggccag
     1621 gattttgttt tcagcacaca gtgccacggc ggccacctga acaatcatca acccagagtt
     1681 caatccggga tgtttggcca gaaacgctgg atgctctgac aaatcagggt tgaccagtcg
     1741 ttcaagccgg cgttcggaaa ggctgccgag ttcagaggca gccatggcgg tgtaatcaag
     1801 cgcaaaggcc accggttcgc catggaaatt cccgccggaa atgacttcac cggtatcggg
     1861 gaacaccagc gggttatcgg tggcactgtt gaattcgatt tcaaggacct gccgggcatg
     1921 ggccagtacg tcacgcaccg caccgtgaac ctgagggata caccgcaggc tgtaggcatc
     1981 ctggactcgt cgacaatctt tgtgtgattc acgaatcagg cttcctgcca acaactggcg
     2041 aaatctggcg gccacctgtc cctgaccggc atgagcgcga actgattgaa ttcgcgggtc
     2101 aaacgccacc agcgttccac gcagggcatc aagtgaaagc gcgccaatca catcggcaag
     2161 gtcagccagt ttttccagtt tgctcagagc cagccctccg acggcagcca tggcctgggt
     2221 gccgttgagc
//
LOCUS       DAOQDO010001039         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45627_length_2228_cov_3.669121, whole genome shotgun sequence.
ACCESSION   DAOQDO010001039 DAOQDO010000000
VERSION     DAOQDO010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45627_length_2228_cov_3.669121"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1704)
                     /locus_tag="PKZ53_15590"
     CDS             complement(157..1704)
                     /locus_tag="PKZ53_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ41912.1"
                     /translation="MQTFLQLLKADLLAQDTHWITLVVLVSVVIIWKYVPTERKYIRN
                     TLLLYGLYFLLVPIAQLLQTVGFLMTGGGLLVVAVLMFRLTLVNVGSVLVFEVFLPVL
                     RISLPRIIRDISILVAYVMVTFSLLAKLGVNITSLITTSAIVTAVIGLSLQDTLGNVM
                     AGLTLQLEHAIQEGDWVKVDEHIGQVKEVRWRFTSIETRNWDTVIIPNSQLVKGQVLI
                     YGKRQKQPLQTRRWIYFNVDFRFPPAEVIERIDTALQKSSIPGVAATPAPHTLLYDFK
                     DSVCQYAVRYWLTDLAVDAPTDSVVRTRIFYALKRAGITPSIPAQRIFTVEENKKWKE
                     RKEEETVQQRLNALRSVDLFHTFNESELRDLSIHLVYSPFSRGEDLTRQGDEAHWLYI
                     LTKGSVSVHVAVKDPVHGELREKEVRRLGPGDIFGEMSLMTGQPRLATVTALEDVECF
                     RLDKDAFQSILIARPQIAEDISHILAKRRLELDATVAGIDEELQQSHVKATQNQMLGL
                     IREFFGL"
     gene            complement(1879..>2228)
                     /locus_tag="PKZ53_15595"
     CDS             complement(1879..>2228)
                     /locus_tag="PKZ53_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNJ41913.1"
                     /translation="VGWMFDYLGIIVLDKDAGSEEQLFELVAGAGADDLKDEDTVWEV
                     LTSPETFNDVYEAIKNAGLSPISAEITMVPKTSVKLEGKDAQAMLKFYDALDDHDDVQ
                     KISANFEVDESEY"
BASE COUNT          562 a          569 c          549 g          548 t
ORIGIN      
        1 gcccatatca ccaaaatcaa tgatctggcc aaacataaag aagaagaaat catgaaggtt
       61 tgagcttgtt tccccagccc ctgatctcca aaacatcatc aaacgcagca aagcggcctc
      121 gataggccgc ttttcatttt tggaggatac gctgagttac aacccaaaga attcacgaat
      181 caatcccagc atttgatttt gggtggcttt gacgtggctt tgctgaagct cctcatcaat
      241 ccccgcgacg gttgcatcca gttcaagccg gcgtttggcc agaatatggg aaatatcttc
      301 ggcaatctga ggtcgggcaa tcaggatgga ttgaaacgcg tctttatcaa gtcgaaaaca
      361 ttcgacatcc tcaagtgctg tcaccgtggc taaccgtggc tgacccgtca tcaatgacat
      421 ttcaccaaaa atatcgcctg ggccaagtcg gcggacttct ttttcccgca attcgccatg
      481 aaccgggtct ttgactgcca cgtgaactga aacggagcct ttggtaagaa tatagagcca
      541 gtgggcttca tcaccctgac gggttaaatc ctcgccccgg ctaaaaggtg aatacaccag
      601 gtggattgac aggtcgcgga gttcagattc attaaatgtg tgaaacagat caacactccg
      661 caaggcattg agccgttgct gaacggtctc ttcttctttg cgttctttcc actttttatt
      721 ttcttcaact gtgaaaattc gctgggctgg gatcgaaggt gtaataccag cacgtttcaa
      781 cgcgtagaaa atccgggttc ggacgactga gtcagttggc gcatcaaccg ccaggtccgt
      841 gagccagtac cgaacggcat actggcagac actgtcctta aagtcataga gcaaggtatg
      901 tggcgcgggt gtggctgcaa cgccaggaat acttgatttc tgaagggcgg tgtcaattcg
      961 ctcaatgact tctgctggag ggaaccgaaa atcaacgttg aaataaatcc agcgccgggt
     1021 ttgcagcggt tgtttttggc gtttgccata gatcagcacc tgtcctttga caagctgact
     1081 atttggaata atcacggtgt cccagttgcg ggtctcaatt gaggtgaacc gccagcggac
     1141 ttctttgacc tggccgatat gttcgtccac tttgacccag tcgccttcct ggatggcgtg
     1201 ttcgagttgc agcgtcaatc cggccatgac attgcccagc gtgtcttgca gtgacaaccc
     1261 aatcacagcg gtcacaatcg ctgaggttgt aatcaggctg gtgatgttca ccccaagttt
     1321 ggccaggagt gaaaaggtaa ccatcacata ggccaccagg atcgagatat cacgaataat
     1381 ccgggggagc gaaatccgca gaactggaag gaagacttca aaaaccagga ccgagccaac
     1441 gttgacgagc gtgaggcgaa acatcaggac tgccaccacc agcagcccac caccagtcat
     1501 cagaaaccca accgtctgaa ggagttgagc gattgggacg agcagaaagt agagcccgta
     1561 cagcagcaag gtattccgga tatacttgcg ttcagtcggg acatacttcc aaataatcac
     1621 cacactgacc agcaccacca gggtgatcca gtgggtatct tgtgccagca ggtcagcttt
     1681 cagtaactgg agaaacgtct gcatttcacc gccttatgag tcggtggact gacaaaaacg
     1741 ggctgaagag gtgagactga aaatgatggg cctgggatga aaacctgttt tttcagcccg
     1801 cttttttcag cccgttttct cagccgcttt cttcagcccg cttttttcag cccgttttct
     1861 tcagtccaat gagaagggtc agtattcgct ttcgtctact tcaaagttgg ctgagatttt
     1921 ctgaacatcg tcgtgatcat cgagcgcgtc ataaaacttc agcattgcct gggcatcttt
     1981 gccttcaagc ttgacagagg ttttcgggac catcgtgatt tcagccgaaa tgggtgacag
     2041 accggcattt ttgatcgctt catacacgtc attaaatgtt tcaggtgagg tcaacacttc
     2101 ccagaccgtg tcttcgtctt tgaggtcatc ggccccggcc ccagccacca gctcaaataa
     2161 ttgttcttcc gatccggcgt ctttgtcgag cacaatgata ccgagatagt caaacatcca
     2221 gccgacac
//
LOCUS       DAOQDO010001040         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45642_length_2228_cov_2.473999, whole genome shotgun sequence.
ACCESSION   DAOQDO010001040 DAOQDO010000000
VERSION     DAOQDO010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45642_length_2228_cov_2.473999"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2228
                     /locus_tag="PKZ53_15600"
     CDS             <1..>2228
                     /locus_tag="PKZ53_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ41914.1"
                     /translation="MSYLLTGEPATIIDELSSVKGGGNALQLTMAYHQRRGKFFRTVE
                     VRDPQFFLRLALIYSRLAQTDFPGEFIYARLGETDYLDCFLYQLVEGAPYLAPTVREN
                     PKLEAELVAQLCVLAGHQPDYIARLAFLANPDSPQTNISEMCQALAGFADYCTHHPEP
                     IREALSAGNQAQSILALKILDTHHIKISDLLPEIIGLVFKSGKKVRELAVAVLQTAKS
                     EAEAWLKTHFAGQKAADQMQSLKLLVDLAGTQAHPTLLTWKKQTTEDKVLVQIDEYVK
                     VFQPVPGVQLPTLVPLLPVETNVPLSPELEQLLIEILPYTSKEVKHPKPEAISEALAK
                     GTVGILEKTSLWPGEWEKFSAVLKKFVTHPQAQLIHIIRLLVMTDNISARYRDTEELF
                     YPESLGAVLLDLYRQSHSPAFDLRQLAAVVKAVGVNSDRVGGTIIHADGGWNRYDIFR
                     YEPEAVWPYFAENLHLLEDVFNPKKILSGYGVEHDQACRRRNALQVLQIFPMPPQSLL
                     PVLWETALGTSKLERPLAQQCLESLPGSFERILSALEDKNLATRQAAVEWLAKLNRPE
                     AIEPLKALLRKEKSEAGRGVLLTALEKLGASVDEFLNRDGLMAEANDGLKKGIPDALS
                     WFPFDQLPALHWSDTGELIDPKIVTWWLVQANKQGTPEPGPILRRYAGLIELAERHSL
                     GQLILEAWIAQDTVPAYTPDQIEQELQKFTQQLSQSSWVAGRIDQYVDERRRDLERQF
                     KG"
BASE COUNT          557 a          585 c          569 g          517 t
ORIGIN      
        1 tcatgtcgta tctgttaacc ggcgaacccg ccacgattat tgatgaactc tccagcgtga
       61 aaggcggtgg gaacgcactc caactgacga tggcgtatca tcagcgccga ggaaaattct
      121 tccgaaccgt ggaagtgcgg gatccacagt tttttcttcg gctggccctg atttatagcc
      181 ggctggccca gactgatttc cctggcgaat ttatttatgc ccggctgggt gaaaccgact
      241 acctggattg ctttttatat caactggttg aaggtgcccc ctatctggcg ccaactgttc
      301 gcgaaaatcc caaactcgaa gcagaacttg ttgcccaact gtgtgtttta gccgggcacc
      361 agccagatta cattgcacgc ctggcctttt tggccaatcc ggactctccg caaaccaata
      421 tttctgaaat gtgccaggcc ctggctggtt ttgcagatta ttgtacgcat catcctgaac
      481 ccattcgcga agccctgagt gctggcaatc aggctcagag tatcctggcg ctcaaaattc
      541 tcgatactca ccacataaag atctctgacc tgttgccgga aatcattggg ctggtgttta
      601 aaagtgggaa aaaagtacgt gaactggccg tggcggtgct ccaaacggct aaatctgagg
      661 ctgaagcatg gctaaaaact cattttgccg gccaaaaagc tgccgaccag atgcaaagct
      721 tgaaacttct ggtggatctg gctggaaccc aggcgcatcc gacgttgctg acctggaaga
      781 aacagacaac tgaagacaaa gtgctggtcc aaattgatga gtatgtgaag gtatttcaac
      841 cggtacctgg agttcaactt cccacactgg tgccgctcct gccagttgag accaatgtcc
      901 ccctgtcacc tgaactcgaa caacttctga ttgagatcct gccctacacc agtaaagaag
      961 taaaacatcc caaacccgaa gctatcagcg aagctctggc caaaggaacc gttggcattc
     1021 ttgagaaaac atcgttgtgg cccggtgaat gggaaaaatt cagtgctgtg ctaaaaaagt
     1081 ttgtgaccca tccgcaggcg caactcatcc acataatccg gttgctggtg atgaccgaca
     1141 atatttcggc ccgatatcgt gacactgagg aattatttta tccagaaagc ctgggggcag
     1201 tacttctgga tttataccgt cagtcgcact cgccggcatt tgatctacga caactggccg
     1261 ccgtggtgaa agccgtcggc gttaactctg atcgagtcgg cggaaccatc attcacgccg
     1321 atggcggttg gaaccggtac gatattttcc ggtatgagcc ggaagccgtc tggccctact
     1381 ttgcggaaaa ccttcatttg cttgaggatg tgtttaatcc aaagaaaatc ctgagtggct
     1441 acggcgtcga acacgaccag gcctgccgcc gtcgaaatgc gctgcaggtc ctacaaatct
     1501 ttccaatgcc accccaatcc ttgctaccgg ttttgtggga aacggcactt ggaacgagca
     1561 aactggaacg cccactggcc cagcaatgcc ttgaatcgct cccaggcagt tttgaacgga
     1621 tcttgagtgc gctcgaagac aaaaacctgg caacgcggca ggcggcggtt gaatggctgg
     1681 ccaaactcaa tcgtcccgaa gccattgagc cactcaaagc attgcttcgc aaagaaaaat
     1741 ccgaagctgg tcgcggggtg ctgttgaccg cgctcgaaaa attgggtgct tccgtggatg
     1801 aattcctcaa ccgcgatggc ctgatggccg aagccaatga tggactgaag aaagggattc
     1861 cggatgcgct ctcctggttc ccatttgacc agcttccggc acttcattgg tctgatacag
     1921 gagagttgat tgatccaaaa attgtgacgt ggtggctggt ccaggccaac aagcagggaa
     1981 ctcccgagcc agggccaatc ctgcggcgct atgctggatt gattgaactg gctgaacgtc
     2041 actcactcgg gcagttgatt ttggaagcct ggattgccca ggacaccgtg ccagcttaca
     2101 cccctgacca gattgaacaa gaacttcaaa aatttactca acaactatct caatcttcct
     2161 gggtagccgg gcggatagat caatatgttg atgaacgccg ccgggatctt gaacggcaat
     2221 ttaagggc
//
LOCUS       DAOQDO010001041         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45668_length_2227_cov_3.910681, whole genome shotgun sequence.
ACCESSION   DAOQDO010001041 DAOQDO010000000
VERSION     DAOQDO010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45668_length_2227_cov_3.910681"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKZ53_15605"
     CDS             complement(<1..243)
                     /locus_tag="PKZ53_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ41915.1"
                     /translation="MAITTCPRCGAENKPTATMCRMCATPLEAPATLVAPANVVGGGY
                     ESPKGPGDMPGPGEVLCGTCGAVNDSSWAFCQQCGSP"
     gene            complement(561..2081)
                     /locus_tag="PKZ53_15610"
     CDS             complement(561..2081)
                     /locus_tag="PKZ53_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ41916.1"
                     /translation="MGLFDRVRSVGQQMASMLDDAKIRREIDRFEKDLLTDPTNIFSM
                     QQLGDLYQAAGLPDKAVSILCQAAELHRSRKEMDLALAFFRKAERLGTSDQRMTILRQ
                     LVDINMQLGRFEDAYQRMRNLLEILINKKEADAAIGLVNALPPLGAKDAQYRKELVEM
                     VNVNRDEWAQGAKGTWVIEGETPPTPEAAMRDTDMKFPDHTLLLVDDEPGVVQLLNAA
                     FNRLGCKILTASNGEEAFNIAVATEPTLIISDLLMPKMDGSQLFAQLRRHPTLSRVPF
                     VCLTSRGQEMERIAALDRGVEDYWVKPFSIAELTIRVRKILQRTRRPADMSGQLAQIA
                     LPELLQMLEGGRKTGILTVQSAGREAHLYFDQGFIVDAEMKDFRGEVVIYKLVYWMRG
                     EFAFRSLPVTRDCKISLNTQQLLMEAMRRYDEASRLIDEEFPDLSATYVCGDSFYTLP
                     IEKEFEPNMARIKKLFNGRNNLRTCCDELNGELETLVLVGELIQQKILEPGQEIEL"
BASE COUNT          548 a          568 c          560 g          551 t
ORIGIN      
        1 cggagaccca cactgttgac aaaatgccca ggaagaatca ttgactgccc cacaggtacc
       61 gcataaaact tcgcctggtc ccggcatgtc cccaggccct ttgggggact cgtatccacc
      121 gcccacaaca tttgctgggg caacaagtgt agctggggcc tcaagcggtg tcgcacacat
      181 ccgacacatg gttgccgtgg gtttgttttc cgccccacat cgaggacagg tcgtgattgc
      241 cattgcgaaa aatctcctct ttctcaggtt atgtgaaagt aagggcccta tatgtagcct
      301 ggaaccaaaa atatgattgc tggaaggtag gtaaggtcgt tgatgaatgc ccgatgatgt
      361 atcggcgaaa tataccacag gcgttgcaaa ccaagacaag gtcaatttat aaggggttga
      421 ggttttaaat aaaggataag gagtgaagaa tgacggaacc cctctgagcc ctggacctga
      481 ggcaaatgta aatcagttga atagctgggc tgacgtcatt ctctatcctg cattctttga
      541 tttttgcgcc taactcctgg ttagagttcg atttcctgtc ctggttcaag gattttctgc
      601 tggatgagtt cacccaccag gaccagcgtt tcaagttcgc catttaattc gtcacaacag
      661 gtgcgcaggt tattgcgccc attaaacagc tttttgatcc gggccatgtt gggttcaaat
      721 tctttttcga tggggagcgt ataaaaactg tcgccgcaca cataggttgc tgaaaggtca
      781 ggaaactctt cgtcaatcaa ccgactggct tcatcatacc gacgcatggc ctccattaac
      841 aattgctggg tgttgagcga gattttgcag tcacgggtga ccggcagtga ccgaaaggca
      901 aactcaccgc gcatccagta caccagttta tagatgacga cttcgccgcg aaaatccttc
      961 atttctgcat caacgataaa cccttgatca aaatagagat gggcttcacg accggctgat
     1021 tgaacagtca aaattccagt ttttcggcca ccttcaagca tctggagcaa ttcaggtaac
     1081 gcgatttgtg ccagttgacc tgacatatcc gccgggcgac gggtgcgctg caagattttg
     1141 cgaacccgga tggtcagttc ggcaatactg aatggtttga cccagtaatc ttcgacacca
     1201 cgatccaggg cagcaattcg ctccatttcc tgaccacgtg aggtgaggca gacaaatgga
     1261 acccggctca gggttgggtg acggcgcaac tgggcaaaaa gctgactgcc atccattttg
     1321 ggcattaaaa ggtcagaaat aatgagggtg ggttcggtcg caaccgcaat gttaaaggct
     1381 tcctcgccgt tactggcggt gaggatcttg cagcccaggc ggttaaaggc cgcgtttagc
     1441 agttgaacca caccgggttc gtcgtcaacc agcagcaagg tatggtccgg aaacttcatg
     1501 tccgtatccc gcatggcggc ctcgggcgtg ggcggggttt ccccttcaat cacccaggtg
     1561 cctttggcgc cttgggccca ttcatcgcgg ttgacattga ccatttcaac cagttctttt
     1621 cgatactggg catctttggc gcccaacggc ggcaaggcat tgaccagccc aatcgctgcg
     1681 tcagcctcct ttttattaat cagaatttcc agcaggttgc gcatgcgctg gtaggcatct
     1741 tcaaaccgac caagctgcat gttgatatca accagttggc gcaaaatggt catgcgctgg
     1801 tcgctggtgc caagtcgctc ggcctttcga aaaaaagcca gagccaggtc catttctttc
     1861 cggctccggt gtaattcagc cgcctggcat aaaatggaaa ccgccttgtc gggcaatcca
     1921 gccgcctgat acagatcgcc aagttgctgc atgctgaaaa tattagtggg atctgtcaaa
     1981 agatccttct caaacctgtc aatttcacga cgtattttgg cgtcatcaag catgctggcc
     2041 atttgttgac caactgagcg aacacgatca aacagtccca tgtggttctt cgcgccaacc
     2101 gttcagcctc aattcaagcc agagccagct ttgaagagca ttgtgaatta tgacaaatga
     2161 accagccagc actctatatt caatgaggta gcttgttcat atttcataat tttaaggcgt
     2221 gagagcg
//
LOCUS       DAOQDO010001042         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45681_length_2227_cov_3.149632, whole genome shotgun sequence.
ACCESSION   DAOQDO010001042 DAOQDO010000000
VERSION     DAOQDO010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45681_length_2227_cov_3.149632"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..590)
                     /locus_tag="PKZ53_15615"
     CDS             complement(60..590)
                     /locus_tag="PKZ53_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HNJ41917.1"
                     /translation="MKSEAVVKTKAEAKPELPIFVKAEHLFEQMEKMYRTIADRAFEL
                     FSHREPNLGNDWEDWFRAESELLRRTPVEMTEADGALKVTIEVPGFKPEDLKVSVEPL
                     SLMVDGKVETTEEKKEEEKTVYTEYRSNRIFRTLPLPKEVDPAQAKATLKDGILTLTL
                     PVVVTEKGTKVEVKTE"
     gene            complement(965..1849)
                     /locus_tag="PKZ53_15620"
     CDS             complement(965..1849)
                     /locus_tag="PKZ53_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ41918.1"
                     /translation="MKILVAIDSSPSSDSVIHQVLSRPWPAHSEICVMTVVDTKAFIP
                     SVPGVESVTRFQVEEAEGLVSRAAAKFSRLGMTASTTISEGYPQAEILEYAKEWKANY
                     LVLGSHGHSRLVRFFLGSTCQTVLRNAPCSVELVRDRRSSPEAVALQPFKVLVATDGS
                     ESAIQALDSIRHRPWPRQTQVRVVSVYDPSVPVADPGFLPSDLISRIQTEHRKMAEDL
                     VASAQRQLAGIEYEVSTHVLEGDAKVRILDEASQWEADLIVLGSHGKRGLVRFLMGSV
                     SEAVALHASCSVNVIRPG"
BASE COUNT          606 a          550 c          519 g          552 t
ORIGIN      
        1 gtaagctgac gatccacgct ttgaagttcg agaaacttca ttcgggaaat ttcaaagcgc
       61 tattcagttt ttacttcaac cttagtgcct ttctcagtca ccacgactgg cagtgtgagg
      121 gttaaaatcc catcctttaa cgtcgccttt gcctgggctg ggtcaacttc ctttggaagc
      181 ggaagcgtgc ggaaaatacg atttgaccga tattcggtgt acaccgtttt ctcctcttct
      241 ttcttttcct cagtcgtttc gaccttgccg tcaaccatca ggctcagggg ttcgacgctc
      301 actttcaggt cttcgggttt aaatccaggc acttcgattg tcaccttcag cgcgccgtcg
      361 gcttctgtca tttcaaccgg ggtccttcgc aacaattctg attccgctcg gaaccagtcc
      421 tcccaatcat tgccaaggtt tggttcccga tggctgaaaa gttcaaaagc acgatcagca
      481 atggtccggt acattttttc catttgctcg aaaaggtgct cagctttaac aaaaattgga
      541 agctccggct tggcttcagc ttttgttttt acaaccgctt cgcttttcat acgacctcct
      601 tttagaaaac tatggagatg caccgtcgca gagtattgag gctgtgccta cgatttttct
      661 taatgcaacc tgtgtgccat tctcaaaaaa acattgaatc gggattgccg atctatccag
      721 agaatactga gtcaatagca aggtaaggtt tatttttcaa agagttaaat taggagttcc
      781 tccatcgaaa aaataagaaa aaaacttgaa aaaagaaagg atgagcgttg atctgactgg
      841 cacctgcctg tgagttcatc gggaattttg aggctcagtg cgcattattt cacgaccaat
      901 tgggaaatct cacccagccc agtaaatatt tttggagtgc accgacttgt cggccctttc
      961 aattttagcc tggcctgatg acattgactg aacatgatgc gtggagtgcc acggcttcgg
     1021 acacactgcc catcaaaaac ctgaccagcc cgcgtttccc atgcgatccc aggacaatta
     1081 aatcagcctc ccattgactg gcttcgtcca aaatacggac cttggcatca ccttcaagca
     1141 catgggttga cacctcatat tcaatcccag ccagttgacg ttgggcagat gccaccagat
     1201 cttcagccat tttccgatgt tcggtctgga ttcgactgat taagtcagag ggcaaaaacc
     1261 ctggatccgc gactggaaca cttggatcat agacactcac aacccggacc tgtgtttgcc
     1321 ttggccaggg gcgatgcctg atcgaatcaa gggcctggat ggcagattca gagccatctg
     1381 tcgcaaccag aactttaaat ggttgtaggg caacagcttc tggactcgac cggcggtccc
     1441 ggaccagttc gactgaacac ggcgcatttc gcaggacggt ttgacaggta ctgcccagga
     1501 aaaaccgcac caatcttgaa tgaccgtgcg agccaagcac cagataattt gctttccact
     1561 ctttggcata ttccaggatt tcggcttgag ggtagccttc agaaatggtc gttgaggcgg
     1621 tcattcccaa ccgggagaat tttgccgccg cccggctgac caggccctcg gcttcctcaa
     1681 cctgaaatcg ggtgacgctc tcaaccccag gtaccgatgg aataaaggct ttggtgtcaa
     1741 ccactgtcat aacacagatt tcggaatgtg ctggccaggg gcgggacaga acctgatgga
     1801 taaccgaatc agacgaaggc gatgaatcaa tggcgacaag tattttcatg gttgcttctc
     1861 cgaaaaaccg aaaattggga aatcaatgag gctgactggc gggatcacct gagtaccgaa
     1921 aaaccacccg gatgaaaccg ttggaaaagg aagttgctgg aatgctataa agaccgtttt
     1981 ctttcccacg gttaccttct gaggataccg tactgtacta agtgcatatc ctgtaccaca
     2041 accaccaaat acaaatgaag aatgaagaat tgagaatgaa gaatgaagaa tgaagaatga
     2101 agaattgaga atgaagaatg aagaattgag aatgcggtga gtggtgagga atcaatatct
     2161 tccaagaacc aggaaccaag aaccaagcac caagaaccaa gaaccaagca ccaggaacca
     2221 agaacca
//
LOCUS       DAOQDO010001043         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45729_length_2226_cov_2.589590, whole genome shotgun sequence.
ACCESSION   DAOQDO010001043 DAOQDO010000000
VERSION     DAOQDO010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45729_length_2226_cov_2.589590"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="PKZ53_15625"
     CDS             complement(<1..1429)
                     /locus_tag="PKZ53_15625"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41919.1"
                     /translation="MANQTVQMQFLTTLVQVDQAWGKVRLAAFYLAIVYLGCLLFSVT
                     VQAQLVAFRFDRFGVDQGLSSQIVTCVLRDHVGYLWIGTESGLNRYDGYTFKVFLPNP
                     KDKKSLRDGSITTVFEDHYGNIWVGTKDGGLHLFDRYTETFHVFQQNPGQSNGLSDNR
                     IYQLYEDPQGVLWIGNVGGLDKIVWDGKTVESIRFTSFRSDPNNPNSLSHSRVTSIVR
                     DQAGTLWVGTRLGLNRMVSENPPRFERFLHDATVPTSLPDNEIYSFLLDREGNLWIGT
                     WGGGGLSKIPAAELSKAQPAFLQFPYDATQNTGLEVDVVFDLSEDAQGNIWLCCRDQG
                     MRYLGRDEQNRPNPRFLSLVPDPGNPFSLASGTAMAMCQDQQGLIWIANLEGGLLKYD
                     PAKSAFHLYRHHPGDVPSLPEGFVTGVAEDRDGSIWVGGSGLTEIVPAGDAFSQPSYI
                     RYLPKPGDPTALQAEDISTLITDSRG"
     gene            complement(1541..>2226)
                     /locus_tag="PKZ53_15630"
     CDS             complement(1541..>2226)
                     /locus_tag="PKZ53_15630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ41920.1"
                     /translation="MTTSTNLKTALEAGAVDYIRKPIDATELEARVGTILKLAESYRI
                     IKVQNQKLEAQKAALEAANTELVRLSTTDPLTGISNRRKFMVVVEEQMKVAERYQSPF
                     TLVMLDIDHFKGINDQFGHDVGDRVLQQVAATIQSSLRTSDTIARWGGEEFMALLQRT
                     RLPIGSEIAEKLREKLASTPVDQVGLISASFGVTEYQGSDTLASLTNRVDEFLYQAKR
                     LGRNRIVAG"
BASE COUNT          570 a          581 c          545 g          530 t
ORIGIN      
        1 tgccgcgtga atcagtgatg agagttgaaa tatcttcggc ctgcagggcg gttgggtcac
       61 caggtttggg taaatagcga atataactgg gttgggaaaa ggcatcgcct gccgggacaa
      121 tctctgtcag accagaccca ccaacccaga ttgacccatc tcgatcttcg gccacgccag
      181 tcacaaatcc ttcggggagg ctcgggacgt cgccaggatg atggcgatac agatgaaacg
      241 ctgactttgc cggatcatat ttcagcagcc cgccttccag attcgcaatc cagatcaatc
      301 cctgttgatc ctgacacatg gccatggcgg taccagatgc caggctaaag gggttgccag
      361 ggtctggcac caaacttaaa aagcgaggat ttggccgatt ctgttcatcc cggcccagat
      421 agcgcatacc ttgatcgcgg caacaaagcc agatattccc ctgggcatct tcagacaggt
      481 caaaaaccac gtctacttca agcccggtgt tttgggtcgc gtcatacgga aactgaagaa
      541 aggctggctg cgctttggat aattcggcgg ctggaatttt cgaaagccct ccgccgcccc
      601 aggtaccaat ccagaggttt ccctcccgat caagcaaaaa gctatagatt tcattatccg
      661 ggaggctcgt tggtacagtg gcatcgtgca gaaagcgctc aaaccgtggt ggattttcag
      721 aaaccatccg gttcagaccc aatcgcgtgc cgacccataa tgtcccggcc tggtcccgaa
      781 caatagatgt tacccggcta tgactgaggc tgttcgggtt gttcggatcg gatcggaatg
      841 atgtaaaccg aatactttca acggttttac catcccagac aatcttatca agcccaccca
      901 cattcccaat ccacagcacg ccttgcgggt cttcatagag ctgataaatc cggttatcgc
      961 ttaacccatt tgattggcca ggattttgct gaaagacgtg aaaggtctcg gtgtatcggt
     1021 caaagagatg taacccgcca tctttggtgc cgacccagat gttcccgtaa tggtcttcaa
     1081 acaccgtggt gattgaacca tcacgtaaac tctttttatc ttttgggttt gggagaaaaa
     1141 ccttgaaggt atatccatca tagcgattta gtccgctctc ggttcctatc cacaaatatc
     1201 cgacatgatc acggagcaca caggtcacaa tctggctgga cagcccctga tcaacgccaa
     1261 accggtcaaa ccgaaacgca accaactgag cctggaccgt gacagaaaac agcagacatc
     1321 ccaggtagac aatcgccagg taaaaagcag ctaagcgcac cttgccccag gcttgatcaa
     1381 cctgaaccaa tgtcgtcaaa aattgcattt gaacagtctg gttggccata gaaaaacctt
     1441 ctgatggtgg ggaatacaac atgaataatg aaagagctaa tcatttgcat atcaaataga
     1501 tagcctcttt ctaattcaga atcaaacaga aggaaaccgg ctaaccagcc acaatccgat
     1561 tgcggcctag ccgtttggcc tgatagagga attcatcaac tcgattggta agtgaagcga
     1621 gtgtgtcgga accctgatat tcggtcaccc caaaacttgc cgaaatcaac ccaacctgat
     1681 ccacgggggt tgaagccagt ttttctctga gtttttccgc gatttcgctt ccgatgggaa
     1741 gccgcgttcg ctgcaaaagc gccataaatt cttcgccgcc ccaccgggca atcgtgtcgc
     1801 tggtccggag cgatgattga atggttgctg ccacctgttg gaggacccga tccccaacat
     1861 catggccaaa ttgatcatta atccctttga aatgatcaat atccagcatc accagggtaa
     1921 atggcgattg ataccgctca gccactttca tttgctcttc gaccacaacc ataaacttcc
     1981 gccggttgga aatcccagtc agtggatcag ttgtggacag gcgaaccagt tcggtgttgg
     2041 cagcttccag ggccgctttc tgcgcttcaa gtttttgatt ttggactttg ataatgcggt
     2101 aggattcagc gagtttgaga atcgtgccga cgcgggcttc gagttcggtg gcgtcaattg
     2161 gtttgcggat gtaatcaacc gcgccagctt cgagagcagt tttgaggttg gtggatgtcg
     2221 tcatca
//
LOCUS       DAOQDO010001044         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45816_length_2223_cov_3.292897, whole genome shotgun sequence.
ACCESSION   DAOQDO010001044 DAOQDO010000000
VERSION     DAOQDO010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45816_length_2223_cov_3.292897"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PKZ53_15635"
     CDS             complement(<1..1079)
                     /locus_tag="PKZ53_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ41921.1"
                     /translation="MAIQGPALSRETDLAELARPTFRFFTDQAGLPQNTINCITVDRK
                     GYLWVGTQDGAAFYNGRQWTVVNMPNRTASNDVTNILAASDGSLWFTTNGGGLANLKD
                     GNWTRFDTTNSGLPDDSLEGLTEIIEPDGHSVLWIGTSQGLVRYEPGRWTVFSTQNSG
                     LPDNLISSILAVTDSSGKQSLWIGSSTGFTCYESGQWKHFENHQEGRGPAQVLQMCTT
                     RSLTGNTVLWVGTNGNGLIRHEAGQSVFLTPRNSGLPDGVIQALCPTISPSGTEALWV
                     GTAGGLAWLEETSQPQGSGFRVQGLEGQALPKYRWKITTTENSNLSSNLISSLFELTN
                     SSGWRTLWVGTYSNGLGRFEEQGWVG"
     gene            1199..1774
                     /locus_tag="PKZ53_15640"
     CDS             1199..1774
                     /locus_tag="PKZ53_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ41922.1"
                     /translation="MSSPPDMTQILQEWNQGDSSALDKLFPLVYDELHRIASRYLRRE
                     RPDHTLQTTALVHEAYLKLLGEQDLQWQNRAHFFGVAARMMRHILVNHALALKTEKRG
                     GGHKVTLNEVIGVPDDRQNWEILALNQALTKLEALDQRKSKVVELKFFAGLSNEEVAA
                     VLDISLATMNREWRLTKAWLVNEIKVCEEGF"
     gene            complement(1912..>2223)
                     /locus_tag="PKZ53_15645"
     CDS             complement(1912..>2223)
                     /locus_tag="PKZ53_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41923.1"
                     /translation="TGILFIADTGNSRILRWVNANPNNSSTFALTGIQMGQVNQPEGV
                     TVKFFSSGSFAGSTLLVVADTGNNRIQGRFLPTGGWSLIGSPNNIGSGIGQFRAPSKA
                     Q"
BASE COUNT          551 a          607 c          549 g          516 t
ORIGIN      
        1 ccaacccacc cctgttcttc aaaccgaccc aggccattgc tgtatgttcc aacccacaag
       61 gtccgccagc cggacgaatt ggtcagctca aacaaactgg aaatcaaatt gctcgaaaga
      121 tttgaatttt cagtggttgt gattttccag cgatacttcg gcagggcctg tccctctaaa
      181 ccctgaaccc tgaaccctga accctgcggc tgtgaagttt cctccaacca ggccagacct
      241 ccggcggtcc caacccagag ggcctctgtt ccagaaggtg aaattgtcgg acacagcgcc
      301 tggatcacac cgtctggtaa tcctgaattc ctgggggtca aaaacaccga ctggccggct
      361 tcgtgtcgga ttaatccatt tccattggtt ccaacccaca gcaccgtgtt tccagtgagg
      421 gaacgggtag tacacatttg caaaacctgg gccgggcctc ttccttcctg gtggttctca
      481 aaatgtttcc actgcccaga ttcatagcag gtaaacccgg tacttgaccc aatccataat
      541 gattgtttcc ccgacgaatc ggtaaccgca aggattgagc tgatgagatt atctgggagc
      601 cctgaatttt gagtcgaaaa caccgtccag cggcccggtt catatcgcac cagtccctgt
      661 gacgtaccaa tccagagcac agaatgtcca tctggttcaa taatttccgt cagtccttcc
      721 agagagtcat ctggcaaacc tgagttggtc gtgtcaaagc gggtccagtt cccatctttg
      781 agattggcca ggccaccacc attggtcgta aaccacagac ttccatctga agccgccagg
      841 atattggtga catcatttga cgccgttcga ttcggcatat tgaccacggt ccactggcgg
      901 ccattgtaga aagcggcgcc atcctgtgtg ccgacccaga gatacccttt tcggtcaacc
      961 gtgatgcagt tgatcgtgtt ttgcggcaaa ccggcctggt cagtaaaaaa gcggaaagtt
     1021 ggtcgagcca gctccgccag atcagtttct cggctgagag ccggtccttg gatagccaaa
     1081 cacacatcag aagctaaccc gtggaagcat aagagaaaga ctgaaatcaa aagagcccgc
     1141 tgcatctttt ccgccagaga agattgcgat atggtgtgat ttggaggaaa agaagggtat
     1201 gagttcgcca ccagatatga ctcagatctt acaggaatgg aatcaagggg attcatcggc
     1261 cctcgataag ctctttccct tagtctatga cgaattgcat cgaatcgcca gccgctacct
     1321 gcgccgggaa cgccctgacc ataccttgca aaccacggcc cttgtccacg aagcctatct
     1381 caaactgttg ggtgaacagg atttacaatg gcaaaaccgg gctcattttt ttggagttgc
     1441 ggcccgaatg atgcgtcata ttctggtcaa ccatgctctg gcactcaaaa ctgaaaagcg
     1501 aggtggaggg cataaagtca ccctcaatga agtcattgga gtgcctgatg atcgtcaaaa
     1561 ttgggaaatc ctggccttaa accaggcact caccaaactc gaagctctgg atcaacgaaa
     1621 aagtaaagtt gttgaactca agttctttgc tggattgagc aacgaagaag tcgctgccgt
     1681 gctcgacatt tcactggcca caatgaaccg cgaatggcga ctgaccaaag cctggctggt
     1741 caacgaaatc aaggtttgtg aggaggggtt ctaaatggtc attccccttc cctttgccca
     1801 tccacccagt cgcctggggc gacggctgga tgggtgggca gggtcagggt cacaactctg
     1861 gggcttcaca ccatcatctt cagccccagc tttcttcaat ccaaccgggc cttattgcgc
     1921 ttttgatgga gcgcggaact ggccgatacc acttccgatg ttatttgggg aaccaatgag
     1981 cgaccagccg ccagttggca ggaatcgtcc ctggattcga ttgttcccgg tgtcggccac
     2041 caccagcaag gttgatccgg caaatgatcc agaactaaaa aacttcaccg ttaccccttc
     2101 tggctggttg acctgcccca tttggatccc cgtcaaggca aaggttgagc tgttgttggg
     2161 attggcattg acccagcgca agattcgtga attgccggta tccgcaataa agagaattcc
     2221 agt
//
LOCUS       DAOQDO010001045         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45909_length_2221_cov_1.862419, whole genome shotgun sequence.
ACCESSION   DAOQDO010001045 DAOQDO010000000
VERSION     DAOQDO010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45909_length_2221_cov_1.862419"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2221
                     /locus_tag="PKZ53_15650"
     CDS             <1..>2221
                     /locus_tag="PKZ53_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41924.1"
                     /translation="NNPQSCLQALQQLAKSKPLAANPQVALFTAEAFRALEKPEIALT
                     WYRQYLKHSAKNPPGYYPVELPEVDQQETVCFRCSSNSLWLPFVCGPGSFRDNLVLRR
                     LIELKAWKEVELEFARIWKHHQIMVGNRAFPGQSLQFVLDYAWFLHTRNLLDQELKVL
                     LTPVSRLDFDHNPIWQYQRSRLVQPVSSAASPQSNNQPPPALFESRCGVTQREFFRLL
                     LEQIQQLKQIPALEQHLNHDLKLGNPCVLLLLAELRSAQEQPEAALKLELDYLKTLAL
                     DQPSRLFREALVFEQYGELKAAIERYSALPGITEVTITLPDVLELDSSVWITKLFQPT
                     FVKNQFQIAKSQLADHMTTLYLMEGDVRKSSTALFQKQARTAVSTSVKELLELRETAL
                     ASSSQTEFEVVEGKTLSRFSFDQQADYAWAIGNWSKAITIQSQYFQRINLPQNWWVPQ
                     DWEERITQLDPALQIKCYELLIEQFPKNAFLRLELLCLKNQLQSEAAIPYLEQLLDET
                     KFPFPYWSTKKNTGLGGYPEVIRMLMSHYVAQCQKEKIFNLALRLARCDPPFKDLVPF
                     LQNKRYSRFENSPMWIETHNVLNLAFDHITSQEQLQILADALQTSPWTNAKTQIAGYL
                     TPLPSSPPSPEFQAQFPVAKKMPGWANLPPGVNLITGMETIFDLCRDETRIYVGHPWG
                     VSIFDFQGNCITRVAIQAPAEHLAVASGALWIGTPRGVLRMDCKTYILSQLPLKTAS"
BASE COUNT          596 a          625 c          464 g          536 t
ORIGIN      
        1 tcaacaaccc acaatcctgt cttcaagcac tccagcagct tgccaaatcc aaacccctgg
       61 ctgccaaccc acaagtagcg ctctttaccg ccgaagcatt tcgagccctc gaaaaaccgg
      121 agatcgccct cacctggtac cgacagtacc tcaaacattc agcgaaaaac ccacccgggt
      181 attatccggt cgaactccct gaagttgatc agcaggaaac agtttgcttt cgctgttctt
      241 caaacagtct ctggctgccg tttgtctgtg gaccggggag ttttcgcgat aatctggttt
      301 tgcggcggtt gattgaactc aaagcctgga aggaagtgga actcgaattt gcgcgaatct
      361 ggaaacacca ccaaatcatg gttggaaatc gggcttttcc aggtcaatct cttcagtttg
      421 tgctggatta tgcctggttt ctccacactc ggaatcttct ggatcaggaa ctcaaagtct
      481 tgctgacacc agtttcacga cttgatttcg atcataaccc catctggcag taccagcgct
      541 cccgacttgt ccagccagtg tcatcagccg cttcgccgca atcgaacaat cagcctccac
      601 cagctctctt cgagtcacga tgtggtgtca cccaacgcga atttttccga cttttgctcg
      661 aacaaatcca acaactcaag caaattccag cacttgaaca acacctcaac catgacctga
      721 aactcggcaa tccctgtgtg ctcctgctcc ttgccgagtt gcgatctgcg caggaacaac
      781 cagaggcagc gctcaaactg gaactggatt acctgaaaac cctggcactc gatcaaccat
      841 cccgattgtt tcgagaagca ctggtgtttg aacaatatgg tgaattgaaa gcggcaatcg
      901 aacgctattc cgcattgcct ggaataaccg aagtcaccat cactttacct gatgttttgg
      961 aactggacag ttccgtctgg attaccaaac tctttcaacc aacctttgtg aaaaaccagt
     1021 tccagattgc taaatctcaa ctggcagatc atatgaccac actctattta atggagggag
     1081 acgtcagaaa atcatccacc gccctctttc aaaaacaagc ccgaacggct gtctcaacca
     1141 gcgttaaaga attattagaa ttgcgtgaaa ccgcactcgc cagcagctct caaactgagt
     1201 ttgaagttgt ggagggcaaa acactctccc gattttcatt tgaccagcag gccgactatg
     1261 cctgggccat cggtaactgg tcgaaggcca tcacgatcca aagtcaatat tttcagcgaa
     1321 tcaacctccc tcaaaactgg tgggtacctc aagactggga agaacgaatt actcaacttg
     1381 atccagcgct tcagatcaaa tgctatgagt tattgatcga gcaatttccc aaaaatgcgt
     1441 ttctgcgcct tgaactccta tgtctcaaga accagcttca aagcgaggca gccattcctt
     1501 acctcgaaca acttctggat gagaccaaat ttccttttcc ttactggtca acgaagaaaa
     1561 atacaggact tggaggctat ccagaggtca ttcgcatgct gatgagccac tatgtggcac
     1621 agtgccaaaa agaaaagatc ttcaaccttg ccctccgatt ggcccggtgt gatccaccat
     1681 tcaaagattt ggtgccattt ctccaaaaca agaggtactc caggttcgaa aattctccga
     1741 tgtggattga gacccacaac gttctcaatc ttgcttttga ccacatcacc agtcaggagc
     1801 aattacaaat cctggccgat gcactgcaaa cttcgccctg gaccaacgcc aaaacccaaa
     1861 tcgccgggta cctgacgcct ttacccagtt caccaccgtc acctgaattc caggctcagt
     1921 ttccagttgc caaaaaaatg cctggctggg ctaatctccc acctggagtc aaccttatta
     1981 ccggcatgga aaccatcttt gacctgtgtc gggatgaaac ccgaatctat gtcggtcacc
     2041 cctggggagt ttcgatattt gattttcagg gaaattgtat tacccgtgta gccattcaag
     2101 ctccagccga acatctggcg gttgccagcg gcgcattgtg gatcgggacg ccacggggtg
     2161 tactgcgaat ggactgtaaa acctatatcc tcagccagtt gccactgaaa acagcgtcac
     2221 a
//
LOCUS       DAOQDO010001046         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45937_length_2220_cov_2.680370, whole genome shotgun sequence.
ACCESSION   DAOQDO010001046 DAOQDO010000000
VERSION     DAOQDO010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45937_length_2220_cov_2.680370"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKZ53_15655"
     CDS             <1..253
                     /locus_tag="PKZ53_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ41925.1"
                     /translation="IPKMEAIQSAAEQRLRPILMTSISTILGLVPLVFASGEGANSRI
                     AMGISVVGGMLISTLLTLYIVPTVYSYVSTKRKHTTTEQ"
     gene            complement(348..1019)
                     /locus_tag="PKZ53_15660"
     CDS             complement(348..1019)
                     /locus_tag="PKZ53_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41926.1"
                     /translation="MVQTMQELVDQIYRETRLMVEITPQTNDLRFLPLGMRSFYAFSD
                     GLSIPFANIYPIYYFKKHLITNTYPVWFEIGDDSSLGGYFCGLVSQEDCYFFSADLAS
                     EICDLPKSKPQFPDLQSLVIDAYRSFTEEAAMCALEIESFPTPEIFQMVCQFFERFDF
                     YPKAEPHRLLAHLPARVIMSPKDAFGIVRHFQPQGAGCFVLPQIVTPHNMREIFNSGA
                     KRTGA"
     gene            complement(1026..1709)
                     /locus_tag="PKZ53_15665"
     CDS             complement(1026..1709)
                     /locus_tag="PKZ53_15665"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41927.1"
                     /translation="MTFWFNLVEKMARDYVSLQSRGEKEKTVYQIVTNWLALLKREDV
                     TTPEVVELVNQAEAKRTFCRDFSVYLADQLKDWAISTGYIPAPQPKVIQVLDFANDGE
                     FPCATVRWKTVGAARYIKQSSCPSRFAVLSLEISPVSNDQNCQLRWYVTGDQIPPEFQ
                     PAVKEAIETYQRNYIETEKVALMFDVAVIFGRYHEVDSNFFSFKVATRMALEKALEAN
                     NLEPIPGRK"
     gene            1814..>2220
                     /locus_tag="PKZ53_15670"
     CDS             1814..>2220
                     /locus_tag="PKZ53_15670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ41928.1"
                     /translation="MSENICIRGTNWVGDSIITIPALRELRRIFPQARLTLVARPWVS
                     GIFEGTGLVDDLLTYDSRSESFWTCVQKIKSRKFDRAILFQNAFEAAALMFCARVPVR
                     VGFPTDARRWLLTQAVPLEPATQVYRCWARLYQP"
BASE COUNT          577 a          541 c          532 g          570 t
ORIGIN      
        1 aatccctaaa atggaggcta tacaatctgc tgccgaacag cggttacgtc ctattctgat
       61 gacaagtatc tcgaccattc ttggtctggt tccgctggta tttgcttccg gcgaaggtgc
      121 caacagccgt attgccatgg gtatctctgt tgtgggaggt atgcttatct ccacgctgct
      181 tacattgtac attgtgccta ccgtgtacag ttatgtctca accaaaagaa aacacactac
      241 aactgaacaa tgatgagaaa tgttagctga tcaatgagcc agcagataat aaaaaggttc
      301 accggatttt gcaatttttc aaatgaatca gttcaataaa agtgtgatca ggctccagtt
      361 ctctttgccc ctgaattgaa aatttcgcgc atattgtgcg gggttacgat ttgtggcaac
      421 acaaaacagc cagctccctg tggttgaaaa tgacgcacaa tcccaaaggc atctttgggt
      481 gacataatca ctctcgctgg aaggtgagcc agcaaccgat gtggctcagc ttttggatag
      541 aaatcgaatc tttcaaaaaa ctggcagacc atctggaaga tttccggggt gggaaaagat
      601 tctatctcca gtgcacacat cgcagcttct tcggtaaagc ttcggtaggc atcaatgaca
      661 aggctttgta gatcaggaaa ttgcggttta gattttggaa gatcacagat ctctgaagca
      721 aggtcagccg aaaaaaaata acaatcttct tgagatacaa gcccgcaaaa atatccgcca
      781 agggatgaat catcaccaat ttcaaaccaa accggataag tgtttgtgat gagatgtttt
      841 ttgaaatagt atatcgggta aatatttgca aacgggatag ataacccatc tgaaaaggca
      901 taaaagctcc gcattcccag gggtaaaaag cgcaggtcat tggtttgcgg agtgatctca
      961 accatgagcc gggtttcgcg ataaatttga tccaccagtt cctgcatcgt ttgaaccatc
     1021 gtgcctcact tccgcccagg tatcggttcc aggttattgg cttccaaggc tttttcaagt
     1081 gccattctgg ttgcgacctt aaatgagaaa aaattgctgt ctacctcgtg ataccggcca
     1141 aatatcacag cgacatcaaa catcagagct actttttcag tttctatgta attacgctga
     1201 tatgtttcaa tggcttcttt cacggctggt tgaaactcag gtgggatttg atccccggtg
     1261 acataccacc gtaactggca gttctgatca ttcgaaacgg gagaaatttc aagcgaaagt
     1321 acggcaaacc gcgacgggca cgagctttgt ttaatgtatc gggcggcacc aactgttttc
     1381 caacgaaccg ttgcacaggg gaactcccca tcattggcaa aatcaagcac ttgaatgact
     1441 ttgggttgag gagcggggat atagccggtt gagatggccc agtctttcag ctgatcggcc
     1501 agatacacag aaaaatcccg acagaaggtg cgtttcgctt cagcctgatt taccaattca
     1561 accacttcag gagtggtcac atcctctcgc ttgaggagtg ccagccagtt ggtcacaatc
     1621 tggtagacag tcttttcctt ctcgccccga gattggagcg aaacataatc gcgtgccatc
     1681 ttttcaacca gattgaacca gaatgtcatg gtgtgtgctc tatcagaatg gggatggtga
     1741 gtttgagact aggcagggcg ctgttttccg atacaatacc ccaaatccca acccctcccc
     1801 ccaaacccta actatgtctg aaaacatttg cattcgcggc accaactggg tgggcgacag
     1861 catcatcacc attcccgcgc tgcgtgaact ccggcgtatt tttccacagg cacgcctgac
     1921 gctggttgcc cgcccctggg tcagtggcat ttttgaaggc accgggttgg ttgacgacct
     1981 gttgacctat gacagccgga gcgaatcgtt ttggacctgc gttcaaaaga tcaaatcccg
     2041 aaaatttgac cgggcaattc tgttccaaaa tgcgtttgaa gccgcggcgc tgatgttttg
     2101 tgcccgggtg ccggttcgcg tcggattccc aaccgatgcc cggcggtggt tgctcaccca
     2161 ggccgtcccg ctggagccgg caacccaggt ttaccgctgc tgggcgcggt tgtaccaacc
//
LOCUS       DAOQDO010001047         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45961_length_2219_cov_3.597043, whole genome shotgun sequence.
ACCESSION   DAOQDO010001047 DAOQDO010000000
VERSION     DAOQDO010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45961_length_2219_cov_3.597043"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..>2219
                     /gene="speA"
                     /locus_tag="PKZ53_15675"
     CDS             487..>2219
                     /gene="speA"
                     /locus_tag="PKZ53_15675"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNJ41929.1"
                     /translation="MKAKLEEIAQTYGIENWGAGYFDINQKGHLIVRPSSGDRRSVDV
                     MELIDDLTRRNIKSPILLRFPQILANQVRKLSSSFQAAIKEFDYKGRYLGVFPMKVNP
                     RREVVEEFLRSGKRYGFGIEVGSKPELYSALALEQSEESLLICNGFKDEAYIRMAFLG
                     TKIGKNVVIVVEKLGELHTILRLTEETGIKPMIGIRVKLYSRGSGKWETSGGETAKFG
                     LTASEVLEVIRLLRDRNSIDQLKLLHFHIGSQITNIKRIKNAMKEAARVYAKIRAMHI
                     DVKYLDVGGGMGVDYDGSKTSSDSSANYTPQEFANDVIYTIKSVCEDEDVPEPNIITE
                     SGRALSAYHAVLITNIHEEIETVVGEYGSVKIDDDDPQVLFELKDVCTGITSKNYTEY
                     YHDALEHKDELFTLFNLGIISLEDRAKGEILFWEVCEKTVKLAQQNKHKPFIDEYEEL
                     SQMLAAKYLCNFSIFRSVPDSWAIEQLFPIMPIHKLNTSPTERATLVDITCDSDGIID
                     KFIDLKDVKETLELHPFKGEPYFLGIFLVGAYQEVMGNGHNLFGAINEAHVLIQDGGY
                     LVTKVVQGSNLG"
BASE COUNT          588 a          504 c          548 g          579 t
ORIGIN      
        1 gtgattgatt tcagattcag accatcaggt tgagtttcaa taagtcacca gccagttcga
       61 cggcttcgga tcaaaccctg aaccctgaac ccgatatact ccaactcttg tggttcgatt
      121 tagcttcacg ctgtagagaa ctgggcaacc gccgatagtg gcctgttcga ttgacgcccc
      181 tttcggcttt tcccggttct ggttcttcaa aaacggctgt caagtcgaaa aatgcatttg
      241 aagcaacttc tcagtaactt ttttagggct gtcgcaatgg gcaaaagaaa tccagggcct
      301 ttcttctaat tatttgtata taaagaggtt gatgtgtgtt tgggtgtgcc tttgatttgg
      361 ctggccggtt gtgccctgga aaaatatttt tttgacactg ctccaaaaga ttgctacatt
      421 tcgcgacttg tgccagaggc tttgaacaac cctccacacc cctcatattc cacttagaga
      481 gacgagatga aagctaaact tgaagagata gcccagacat acggcataga aaactggggt
      541 gccggctact ttgacattaa ccagaaggga catctcattg tccgcccaag ttcgggagat
      601 cggcgttctg tagatgtgat ggaactgatt gacgatctga cccggcgtaa catcaaatct
      661 cccatcttgc tccgttttcc ccaaatcctg gccaaccagg tccgcaaact ctccagttca
      721 tttcaggctg ccattaaaga atttgactac aaaggacgtt acctcggtgt tttccccatg
      781 aaagtgaatc cccggcgtga agtcgtcgag gagtttctgc gcagtggtaa acgctatggg
      841 tttgggattg aagtcgggag caaaccagaa ttgtattcag ccctggcttt ggagcaatct
      901 gaggaatcgc tcctgatttg caatggcttc aaggatgaag cctacatccg gatggccttt
      961 ctcggtacca agattgggaa aaacgtggtc atcgtggttg aaaaactggg tgaacttcac
     1021 acgatcctgc gactgactga agaaaccggc atcaagccca tgattggcat ccgggtgaag
     1081 ctctattccc gaggaagcgg gaaatgggaa acttccggtg gtgaaacggc caagtttggg
     1141 ttaaccgcca gtgaagttct ggaagtgatc cggttgttac gtgaccgcaa ttcgattgac
     1201 cagctcaaac tccttcattt tcatattggt tcgcagatta ccaacatcaa acgcatcaaa
     1261 aacgcaatga aagaggccgc acgagtctat gccaagattc gggcgatgca cattgacgtc
     1321 aagtatctgg acgttggcgg tggcatgggg gtggattacg acggcagcaa aaccagctct
     1381 gactcgtcag ccaattacac accacaagaa tttgccaacg acgttattta caccatcaaa
     1441 agcgtttgtg aggatgagga cgttccagag ccaaacatta tcaccgaatc cgggcgtgcc
     1501 ctttcggcct atcacgcggt gctcattacc aatatccatg aagaaattga aacggttgtg
     1561 ggtgagtatg gctcggtgaa gattgacgat gatgatcccc aggttttgtt tgaactcaag
     1621 gatgtatgca ccgggatcac ctcaaagaac tataccgagt attaccacga cgcgcttgag
     1681 cacaaagacg aactgtttac cttgtttaat cttggcatca tcagcctgga agatcgcgcc
     1741 aaaggtgaaa ttttgttttg ggaagtttgc gaaaaaacgg tcaaactggc tcagcaaaac
     1801 aaacacaagc catttattga tgaatatgaa gaacttagcc agatgctggc tgccaagtat
     1861 ttgtgtaact tttcgatttt ccgatctgtt cctgatagtt gggcgattga gcaactgttt
     1921 ccgattatgc cgattcacaa gctcaatacc agtcccaccg aacgcgccac cctggtggat
     1981 attacctgtg actcagatgg gatcatcgac aagtttattg atttaaaaga tgtgaaggaa
     2041 accctggaac tgcatccatt caaaggggag ccgtatttcc tgggtatttt tttggtggga
     2101 gcttaccagg aagtgatggg aaatggtcac aacctgtttg gagccattaa tgaagctcac
     2161 gttttgatcc aggatggcgg ctatctggtc acgaaagtgg ttcaggggag caatcttgg
//
LOCUS       DAOQDO010001048         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_45969_length_2219_cov_2.835952, whole genome shotgun sequence.
ACCESSION   DAOQDO010001048 DAOQDO010000000
VERSION     DAOQDO010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45969_length_2219_cov_2.835952"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKZ53_15680"
     CDS             <1..572
                     /locus_tag="PKZ53_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41930.1"
                     /translation="VRTYQNQSKGAATQVQGSGSSPLATKPLDLLPSQELIETAAKSA
                     GGADRKPPSEPLTARPVVASIKPTPAALPQLTQPAQPPPKTTPKPTTAPVPDRSATTT
                     AAQRKISSSPTGPRQKAEDVSISPNRTGANVPVSRAESVQTPAYRSSYAETADSTSSD
                     EKRQQMMIIGIGVAIVIVFILILLFVALS"
     gene            complement(605..1558)
                     /locus_tag="PKZ53_15685"
     CDS             complement(605..1558)
                     /locus_tag="PKZ53_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ41931.1"
                     /translation="MKLLTKVVLSAVLLVLGLIPGWGCQFPEPSQPEIPFPAWQPPTP
                     PANTKTRLLPKPGRGHNIFKGDAEHWLVDYLTPEGTELEDPQVSAYLDELANYLASFC
                     PEPNRRFQVRVLTDELPDAFCQGDGKIYISVGFLKMMEREDELAGVLAHEMGHDVLKH
                     IPKTLSRQLFWMTGTTKITSREQLNLVLTRLAEKYEKMPVAAFMERLSGISRMDELKA
                     DQCAVATLFRAGYNPQALITILARAERYQKEAYGRGYGKAKFVQFWLGSHPPTNIRKT
                     AFQLERMFIKLPPKDTYHPSQAFEAMKTRLNKVVNSEEEEK"
     gene            complement(1719..>2219)
                     /locus_tag="PKZ53_15690"
     CDS             complement(1719..>2219)
                     /locus_tag="PKZ53_15690"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ41932.1"
                     /translation="IDYLYEYYNLKPGKNRFFTSNNLALAIEAFHTLGGFDADSFSKP
                     AGEDRDLCSRWNALAYPMIYLPHAHITHSHELTFKTFWKQHFTYGRGAYAFHHARARR
                     SQHQLAVEPFSFYWDLLFYPFRQYPFFSACHFSLLFVISQLANTSGFLWEWKSGPKKP
                     GLRAGG"
BASE COUNT          546 a          590 c          555 g          528 t
ORIGIN      
        1 cagtccgaac ttaccaaaat caaagtaagg gcgccgcgac ccaggttcag ggttcaggct
       61 cctcgccgct ggcaaccaaa ccactggatt tgctcccctc acaagagttg attgaaacag
      121 ccgccaaaag cgctggcgga gcagatcgaa aaccgccatc tgaaccactg acagcacgcc
      181 cggttgtggc ctcgatcaaa ccgactccgg cggcactgcc ccaactgacc caacccgctc
      241 aaccaccacc aaaaacgact cccaaaccga cgacggcacc tgttcctgac cggtccgcca
      301 ccaccaccgc tgctcagcgc aaaatttcat ccagccccac cggcccacgg caaaaggcgg
      361 aagatgtcag tatttccccc aatcgaactg gcgccaatgt cccggtttca agggctgaat
      421 ctgtccagac accggcctat cgttcatcct atgctgaaac tgccgatagc acgagcagtg
      481 atgaaaaacg ccagcaaatg atgattattg ggattggggt tgcaattgtg attgtcttta
      541 tattgatttt gctcttcgtc gccctgtcct gaagggcaaa atagtaaaac cccctgtcat
      601 tctgtcattt ttcctcttcc tcagagttca caactttgtt gagccgggtt ttcatggctt
      661 caaatgcctg actgggatgg tatgtgtctt tgggagggag cttgataaac attcgttcca
      721 gttgaaatgc cgttttgcgg atatttgtgg gtgggtggct gccgagccag aactgaacaa
      781 attttgcttt cccatagccc cttccatagg cttctttttg atagcgttcc gcccgtgcca
      841 gtatcgtgat caaggcttgc ggattatacc cggcgcgaaa gagagtggcg acggcacact
      901 ggtcagcttt gagttcatcc atccggctga taccactcaa ccgttccata aatgctgcca
      961 ccggcatctt ttcatacttt tcagccaggc gggtgaggac cagattcagt tgttcgcgag
     1021 aggtgatttt ggtggtgcca gtcatccaga acaactgacg gctcagtgtt ttcggaatat
     1081 gtttcagcac atcgtgcccc atctcatgcg ccaggactcc ggccagctcg tcttcgcgtt
     1141 ccatcatttt tagaaatccg accgaaatat agattttgcc atctccctga caaaatgcgt
     1201 ccggtaattc gtctgtcagc acccgtacct gaaatcgccg gtttggttca gggcaaaaac
     1261 tggccagata attggccagt tcgtccaaat aggcggaaac ctggggatct tcaagctcag
     1321 ttccttcagg cgtcaggtag tcaaccagcc agtgttcggc atcacctttg aagatattgt
     1381 gacctcgacc cggtttgggt aacagccggg ttttggtgtt ggctggtggc gttggcggtt
     1441 gccaggctgg aaagggtatt tctggttgag atggctcggg gaactggcac ccccaaccgg
     1501 ggatcaaccc caataccaga agcacggcac tcagaacaac tttggtaaga agtttcataa
     1561 aatcacccgc ctgaagagtt ggggctgaag aatatcgcgc tgaaaacctc gggctcaggg
     1621 cttggggctg aagattcgca agctcagggc tgaagaaagc tgggaaaacc aattcttcaa
     1681 cccgatgtct tcaacccaat ttcttcagcc cgatgtcttc agcccccagc tctgagccct
     1741 ggttttttcg gccctgattt ccattcccat aaaaacccgc tggtgttggc cagttgagag
     1801 atcacaaaca gcaaagaaaa gtgacaggct gaaaagaacg ggtattgtcg aaacggataa
     1861 aaaagcaaat cccagtaaaa tgaaaaaggt tcaaccgcca actggtgctg gcttcgtctg
     1921 gctcgggcat ggtgaaaggc ataggcccca cggccatagg taaagtgctg tttccaaaag
     1981 gttttgaacg taagttcgtg cgaatgtgtt atgtgcgcgt gcggaagata gatcatcgga
     2041 taggctaatg cgttccatcg agagcacaaa tcacggtctt cgcctgccgg tttggaaaac
     2101 gagtctgcgt caaacccacc cagtgtgtga aacgcctcaa tcgccagcgc caggttgttg
     2161 gaggtgaaaa atcggttttt cccaggtttc aaattatagt actcgtacag ataatcaat
//
LOCUS       DAOQDO010001049         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46007_length_2218_cov_3.033287, whole genome shotgun sequence.
ACCESSION   DAOQDO010001049 DAOQDO010000000
VERSION     DAOQDO010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46007_length_2218_cov_3.033287"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PKZ53_15695"
     CDS             <1..1512
                     /locus_tag="PKZ53_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ41933.1"
                     /translation="YPKSETLGGAHLNAIGTLRLAGDTTGALDWCARTETRLSTEVAG
                     VTAKFLRAKILMNLNQFQAALNVLNGLQNEPLGRRAPGATNRGEVSFLRGYCLEQLKQ
                     YSPAIDVYLGLPDERNQYYGQRATERLMNLRATLKGNAANEFTRRLTTLLTTARTAST
                     AGRYGEAKYAAHQALRLTSDPATERELFRMLETCYRNLPDYARAWNYQLVPVGRNVIT
                     SASSSPAQRDLGDELCFLGLFDEGISELPAPRVKAGGNDNWNYSLAVYANRGNRAWQA
                     IDYAERVFGSLPADFRLELMPRDVAELLYPAPYRPAMLKATRPRQVDPRLLLAIARQE
                     SRFRPWVKSAAAARGLFQFIDSTADQIATQLKLDTYSRDDLYEPKYAVLFGAQYVSNL
                     FKQFPGHPQAVVASYNGGEAGVRRWLQRAGSDDVDRFTPEVGFAETKDYVFIVMNNYA
                     AYRQLYQRDLTEPEASQSASTIPQTQDEATSSYLHDLALALVEGGAVALLKEQ"
     gene            1748..2149
                     /locus_tag="PKZ53_15700"
     CDS             1748..2149
                     /locus_tag="PKZ53_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41934.1"
                     /translation="MNIIVMTYLTYLVLSIILTIWVAQTLHKNGRVFLVDVFHGNIEL
                     ADSVNHLLVVGFYLINLGYISLALKLGYQVDSTQVGIEALSSKIGTVAIVLGGMHFFN
                     LYIFSRLRRRAMLDNVPPPVMPDACINCHEV"
BASE COUNT          554 a          596 c          572 g          496 t
ORIGIN      
        1 tacccaaaga gtgaaaccct gggcggtgcg cacttaaatg caattggtac cctgcgactg
       61 gcaggagaca cgacaggagc acttgactgg tgcgcccgca cggaaacacg actgtcaacc
      121 gaagtggccg gggtgacagc gaaattctta cgagccaaaa ttctgatgaa tttgaaccag
      181 ttccaggcgg cgctcaatgt cctgaatggg ctccaaaatg agccacttgg acgtcgagca
      241 ccgggcgcaa ccaatcgcgg cgaagtgagt ttcctgcggg gctattgcct ggagcaactc
      301 aagcaataca gtccggccat tgacgtctac cttgggcttc cagacgaacg gaatcaatat
      361 tatggccagc gagccaccga acgcctgatg aacctgcgcg caacactcaa aggcaatgcg
      421 gccaatgaat ttaccagacg actgacgacc ttgctgacca cggctcgaac ggcaagcacg
      481 gcgggacgct atggggaagc caaatatgcc gctcatcagg cgctccgatt gaccagcgat
      541 ccggccaccg aacgcgagct gtttcgaatg cttgaaacct gctatcgcaa cctgcccgac
      601 tatgcccggg cctggaatta ccagcttgtt cctgttggcc gcaatgtcat caccagtgcc
      661 agcagcagtc cggctcagcg tgatttgggt gatgaactct gcttcctggg tctgtttgat
      721 gaaggtattt ctgagcttcc agccccacgg gttaaagcag gcggaaatga caactggaat
      781 tattccctgg cagtatatgc gaaccgggga aaccgggcat ggcaggcaat tgattatgcc
      841 gaacgggttt ttggatcgct cccggctgat tttcggctcg aattgatgcc gcgtgatgtg
      901 gccgaactgc tctatccggc accctatcgg ccagccatgc tcaaagccac gcgtccacga
      961 caggttgatc cacggctgtt gctggcaatt gcccgccagg aatcgcgctt ccgcccgtgg
     1021 gtgaaatcag ccgcggcggc tcgcggtcta tttcagttta ttgactcaac cgccgatcag
     1081 attgccactc aactgaaact cgatacctac tcacgagatg atttatacga acccaaatat
     1141 gcggtgttgt ttggcgccca atacgtttca aacctgttta agcagttccc tggccatcca
     1201 caggcggtgg tggcctcata caatggtggt gaagcggggg tccggcgatg gctgcaacgg
     1261 gctggaagtg atgacgtaga ccggtttacc cccgaagtcg ggtttgctga aaccaaggac
     1321 tatgttttta ttgtcatgaa caactacgcc gcttatcgcc aactgtatca acgagatctg
     1381 accgaaccag aagccagcca gtcagcctcg accatcccac aaacccagga cgaagcaacc
     1441 agttcctatc tgcacgatct ggcactggca ctggtcgaag gtggggcagt ggcgcttctg
     1501 aaagaacaat aaacctccaa gcattcaaag cattttcaac cacgaaatac acgaaataca
     1561 cgaaaagaat caaacactta caaaattcaa ctgctcagca acctttggac aaggtatata
     1621 tgtggagtgc ggcggcttga cgccgccttt attttttgaa cgtgttcaaa aaactattga
     1681 cacctacctt cggacagcgt actctctttt tgaacacgtt caaaaaaatc ttcaaagagg
     1741 tagctgtatg aacatcattg taatgaccta cctgacctat ctggtcctga gtattatttt
     1801 gacaatttgg gtggcgcaga cgctccacaa aaacggacgc gtttttctgg tggatgtgtt
     1861 tcacggcaac atcgagctgg ccgactcagt caaccatttg ctggtggttg ggttttattt
     1921 gatcaacctg gggtatatca gtctggccct gaaacttggg tatcaggttg acagcacaca
     1981 agttgggatt gaagcgttat cgtcaaagat tgggacagtc gccattgtcc tgggcggcat
     2041 gcacttcttt aacctctaca ttttcagccg gttgcggcga cgggcgatgc tcgacaacgt
     2101 tcctccgccc gtcatgccag atgcctgtat caactgccat gaggtatgac aaggtgacaa
     2161 agtgaccaga tgacatttat tcgaaaaccc ggaacccgga acccggaacc cggaaccc
//
LOCUS       DAOQDO010001050         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46015_length_2218_cov_2.851133, whole genome shotgun sequence.
ACCESSION   DAOQDO010001050 DAOQDO010000000
VERSION     DAOQDO010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46015_length_2218_cov_2.851133"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKZ53_15705"
     CDS             <1..477
                     /locus_tag="PKZ53_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41935.1"
                     /translation="IHGVSHWARVRDNGLRLAKVTGANPTVVELFALLHDSQRQSDTC
                     DDDHGWRAAVFAAQLQGKFYQLPPDDLRLLQLACQDHSRGRTQGDITVLTCWDADRLD
                     LGRVGIPPLAKYLCTAPAKEPKMIEWAYNRSLKWKERFGGDDWLPVMLEELLEQPE"
     gene            801..1574
                     /locus_tag="PKZ53_15710"
     CDS             801..1574
                     /locus_tag="PKZ53_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ41936.1"
                     /translation="MLDWFIHWLQTASWLAASLVFLVENLVILGLVIVIGNWMVVLFQ
                     HRRVALPPLPLTCAEVALALLNVGLNTIVTLAGWQLWRHGVIHFRSDFGFGALADVFV
                     LLMVMDFLMYTLHRVAHHRWLYSVMHQLHHRYERLRPLTLFALNPIENLGFGMLWLMV
                     ITVYPVSWAGMSVYLGFNVAFGTIGHLGVEPVPGDWAQKPVFQAIAGSSFHAQHHQDI
                     NYNFGFYTLIWDRLFGTIQPHYQLKYGQIPAVEVNKAGR"
BASE COUNT          530 a          534 c          540 g          614 t
ORIGIN      
        1 atccacggcg tgtcacactg ggcacgcgtt cgagataatg gattgaggct ggccaaagta
       61 accggcgcca acccgaccgt ggtggaactc tttgccctgc ttcacgattc acagcgccag
      121 tcagacacct gtgatgatga ccatgggtgg cgcgctgcag ttttcgcggc ccagttgcag
      181 ggaaaatttt accaactccc gccggatgac cttcggctgt tacagcttgc ctgccaggac
      241 catagccgtg gcagaaccca gggcgatatt acggtattga cctgttggga tgctgaccgg
      301 cttgaccttg gtcgcgtcgg aatccctcct ctcgccaaat acctctgtac ggctccagcc
      361 aaggaaccaa aaatgattga gtgggcttac aaccgtagtc tgaaatggaa agagcgattt
      421 ggcggagatg actggttgcc agtgatgctt gaagaactcc ttgaacagcc agaatgatga
      481 tcagggaaca aggcgatatt ccccagagtg atccctctca gcccgtattt ctgttacagc
      541 tctagctttt cagataaata tttcctggga gcgttggcac cctgccggca ccacgtggtt
      601 catatccaat cagttgccgg caaaataccg gtgttcccag agggaatctt tttctgaaag
      661 actatatgtc attcctcgat atagaattgc gacccgaagt tgtacctgcg actcgatagt
      721 tactcttgaa cgctttaacc gggcaggtat tgtttgtctg gtaccctgaa ctcaacccct
      781 cagaccctga agatatatct atgctcgact ggtttattca ttggctccaa actgcttcct
      841 ggctggcggc aagcctggtg tttctggtcg agaatcttgt gattctaggg ctggtgattg
      901 tgatcggaaa ctggatggtt gtgttgttcc agcatcgccg ggtagccttg cctccactcc
      961 ccttgacctg tgccgaagtc gcgctggcgc tcctgaacgt agggttaaac accattgtga
     1021 cactggctgg ttggcaactt tggcgacacg gcgtgatcca ctttcggtct gacttcggat
     1081 ttggcgcact ggctgatgtg tttgtgttgc tgatggtgat ggatttcttg atgtatacac
     1141 tccatcgcgt tgcccatcac cggtggcttt attctgtcat gcatcaactc catcaccgct
     1201 atgaacgact ccgcccgctg acacttttcg ccttgaatcc aattgaaaac cttgggtttg
     1261 ggatgctttg gctgatggtc atcacagtct atccagtttc ctgggcggga atgtcagtct
     1321 atttaggatt caatgtggcc tttggcacga ttggtcattt gggggttgag ccagtgcctg
     1381 gtgactgggc ccaaaaacca gtgtttcagg ctatcgccgg tagttccttt catgcccaac
     1441 accaccagga tatcaactac aattttggtt tttatacact gatttgggat cgcttatttg
     1501 gaacgataca gcctcactat cagctcaaat acggccagat tccagccgtg gaagtcaata
     1561 aggcaggcag gtgacgacga ttcaaactac cggggaaaaa acaccaggca gcctcatcaa
     1621 acgaccagtt gaccaaagct caggttgtga atgattcgga aaaaaataaa aaactgtgag
     1681 catgggaaat cagaaattac gcgtttgaga atgaaggtca aaatttatgt gtatcccacc
     1741 gtactttctg tggaggttgc tctatctcta attatttgaa tacctgatgc aatttggctg
     1801 attgctctca gtttgacaca ttggcgaaac actgagggtt attcgccatc aaatgggtca
     1861 gtgacctgtt cgcaccactg gtctgatggc aagacatctg atgaaaatcc attcaaatca
     1921 ggattctatc gatagaaaca attctatttg gcttttggcg aaagtctgtt tctagttcag
     1981 aaaataaacc tggcactggc ctggtgatgg agattgttat gtcttcccca tctctcggcg
     2041 gcgccctgtg ttttattatt tttaaaccag tgactacaac tctcgccagt ataacggttc
     2101 cacttgggaa tgatggccaa ataatgctct ttagggctaa gagctaccag agatatacaa
     2161 tctctaacta tttagctttt tgctcataca acatacttat ttcaaaatgg tgtaacga
//
LOCUS       DAOQDO010001051         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46017_length_2218_cov_2.805825, whole genome shotgun sequence.
ACCESSION   DAOQDO010001051 DAOQDO010000000
VERSION     DAOQDO010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46017_length_2218_cov_2.805825"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2218
                     /locus_tag="PKZ53_15715"
     CDS             <1..>2218
                     /locus_tag="PKZ53_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41937.1"
                     /translation="SPPFNSPIQRTLDVDVPYKGRTKYHRKSAIEKNVPHNPYKNIIV
                     NMLYYWAQLNEELSFDSWEDAIQKAMVAIYDLKDHPRLNEISELLEDPEFDKTNIRKL
                     QLILQQAFESEDLSPTKVEPIDVKKTETVDVIDMSGKLLKQEEIVHPIFQDITQGKYS
                     FVIVQLPDEVDEETLQTPVKYKGKNYRIDLMAGSSIKPAKGKSSTVYGIEEDSVEMFK
                     DMFGFEESSYYAQRSLFPLVNAGVTGKIRSRAYPIEKGMEHTFRLKDGTEIQVDDGWG
                     YIKKSLADKMQQGGMQYEKRQPVANTGKASHQMMEWFDEENIDVINELVQWGLSQWVQ
                     GYQEFVQQKHNVGTNEYFQSKRSVMSLLTTGKPPMEMGVAMPVPGKNVVIPKDQTRYT
                     KHLPGEMSLIRSPVDKQNFRPISAKNIEQNTKISKLVGNIEGIQYTLTGRQDDLLTFF
                     KGMVGVIDDSLWPVDWKGVDLVVSSKDRKLYEKWVRDTSLEEDAEDVVDRETDRSSMG
                     KAIQDFTIEGTLAAIQWFTKGSFIGVPANIQKWLGGDYDGDEVAAIFGKTNPQLQAYI
                     RDKAYQELEINPKLQKTFTYNPDSSRAKRMVAMRSPNVGNWSNIAAWVRSLPIELKGW
                     LAEETADGRLLPDDEEFSSLDETQKMEKEIQLGIKVGTDAYKTSTNVKAFEQRASLYL
                     NLLKAYARPIMHNKKLLDVFDKVGVLPTLKEPAWRDMFFSYDEVNKNTGEYLIKGVS"
BASE COUNT          685 a          479 c          549 g          505 t
ORIGIN      
        1 ggtcaccccc attcaattca ccgattcagc gcacgctgga tgtggatgtt ccttacaaag
       61 gaagaaccaa ataccaccgc aagagtgcga ttgagaaaaa tgtacctcat aatccataca
      121 aaaacatcat cgtcaatatg ttgtattact gggctcagct caatgaggaa ctctcatttg
      181 actcctggga ggacgccatc caaaaagcga tggtggcaat ttatgatctg aaggaccatc
      241 ccaggctcaa cgaaatctca gagttacttg aggatcccga gtttgacaaa accaacattc
      301 gaaaattgca acttattctg caacaggcat ttgagagtga ggatctgtct ccgaccaaag
      361 ttgaaccaat tgatgtcaag aaaacagaaa cggttgacgt gattgatatg agtgggaaac
      421 ttctgaaaca ggaggaaata gttcatccaa tattccagga catcacccag ggaaagtaca
      481 gctttgtcat tgtacaattg ccggatgaag ttgacgaaga gacgcttcaa accccagtga
      541 aatacaaagg caagaactat cggattgacc tcatggcggg atcttcgatc aaaccagcca
      601 agggaaaatc cagcaccgtc tatggtattg aagaagatag cgtcgaaatg tttaaggata
      661 tgtttgggtt tgaagagtca tcttactacg cccaacgatc tttattccca cttgtgaatg
      721 ccggggtgac ggggaaaatc cgaagccggg cctacccgat tgaaaaagga atggaacaca
      781 cgtttcggct caaggatgga accgaaattc aggtggacga tgggtgggga tatattaaaa
      841 agagtctggc cgataaaatg cagcaaggtg gtatgcaata tgaaaaacgg cagcctgtcg
      901 ccaacactgg aaaagccagc caccagatga tggaatggtt tgacgaagaa aatattgatg
      961 tcatcaatga gctggtccag tggggcttat cccaatgggt tcaagggtat caggagtttg
     1021 tgcagcaaaa acacaatgtt ggaacgaatg aatatttcca atccaagcgc tctgtgatga
     1081 gcctcttgac caccggcaaa ccaccgatgg aaatgggtgt cgcgatgccc gtgccgggga
     1141 aaaatgtcgt gatccccaaa gatcaaaccc gctataccaa acaccttcca ggtgaaatgt
     1201 cgttgattcg ctcgccggtg gataagcaga actttcggcc catttcagcc aaaaatattg
     1261 aacaaaacac caaaatctca aagctggtcg gcaatatcga aggcattcaa tacactctga
     1321 ctgggcgcca ggacgatttg ctcacgtttt ttaagggaat ggttggtgtg attgacgatt
     1381 cgttgtggcc cgtggattgg aaaggcgtag acctggtggt atcgtcaaaa gatcgaaagc
     1441 tttacgagaa atgggttcgt gacacttcac ttgaggaaga cgctgaagac gtggttgacc
     1501 gtgaaacaga ccgttcctcg atgggtaagg caatccagga ttttacgatt gaaggcacac
     1561 tcgccgccat ccagtggttt accaaaggca gttttatcgg tgttccagcc aatatccaaa
     1621 aatggcttgg cggcgattat gacggtgatg aagtggcagc catctttgga aaaaccaacc
     1681 cgcaacttca agcatatatt cgggataaag cctatcagga actggagatc aatccaaagc
     1741 tccaaaagac atttacttac aatccagatt cgagccgtgc caaacgcatg gtggcgatgc
     1801 gttcgcccaa cgtcggcaac tggtcaaaca ttgcggcgtg ggttcgatca ttgccgattg
     1861 aactgaaagg ttggctggct gaagaaaccg ccgacggacg actgttacca gatgacgaag
     1921 aatttagcag tctggatgaa acccagaaga tggaaaaaga aatccagctt ggtatcaaag
     1981 tcggcactga tgcgtataaa acatccacca atgtcaaagc ttttgaacag cgagccagtt
     2041 tgtacctcaa tctgttgaaa gcttacgcca gaccgattat gcacaataaa aaactgctgg
     2101 atgtgtttga taaagttgga gtgcttccaa ccctgaaaga gccagcctgg cgggatatgt
     2161 tcttcagtta tgacgaagtc aacaaaaata ctggcgaata cctgattaaa ggtgtgtc
//
LOCUS       DAOQDO010001052         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46119_length_2216_cov_1.624248, whole genome shotgun sequence.
ACCESSION   DAOQDO010001052 DAOQDO010000000
VERSION     DAOQDO010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46119_length_2216_cov_1.624248"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..499
                     /locus_tag="PKZ53_15720"
     CDS             218..499
                     /locus_tag="PKZ53_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNJ41938.1"
                     /translation="MPIQTTYTDARANFAKLCDQVTSDREVAIITRRGAEDVALISAS
                     ELSSLLETAHLLRSPKNADRLLTALKRAQERTVSSQTVDELRQELGIDE"
     gene            492..755
                     /locus_tag="PKZ53_15725"
     CDS             492..755
                     /locus_tag="PKZ53_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNJ41939.1"
                     /translation="MSDRDAVFQPEFREDLQFWVETNRKTALRAFALIEATMRDPFEG
                     IGKPEPLKYLGANVWSRRLTQEHRIVYLVSDDRIDFLQARYHY"
     gene            914..2212
                     /locus_tag="PKZ53_15730"
     CDS             914..2212
                     /locus_tag="PKZ53_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41940.1"
                     /translation="MNIKIKQGEPWSLAGMINNFETICDTEGRPVFEGRAPVGVKEKV
                     PMVMRKCPYPDERQGRMMNVSALNQITEHLDAALENIASYRLTLPSTVTSWDFFYRAI
                     LDQLGQAALYILRNKPVLPVQLSVTYKVATGYQVPLRELLNLELSRQAPVPTVANLLD
                     YVKQRKALVGKREVCAAPMSMVTKVTEVLYDGLPDQKTKARPERVAVAQALMLQTWLG
                     IVWRRFDLAAERQLLLEDLGPATLFPRTPYLKRLLADRLGEIEQSEVVDYFELPHLLP
                     PDLRQSLRRTMAFLEGGQELCGETVSLIQSLMEHDDAVVVMSDLTRRPVFAQRFVAYL
                     KAYRSFVAHFEAIEIDLRQRLVFPTEPPVLLDPSFFPSPRALKWFELVSGHLIRPNPE
                     VSGGLMFQNQHRSIPIPLIPVSKNQGLGFGAEENGLKKAD"
BASE COUNT          526 a          536 c          601 g          553 t
ORIGIN      
        1 ggtttatctg ggagaagacc gtggagaaat caaaaaagcg atgaaaaacg tgttttcaaa
       61 ggatctgatt tacaaatctg tcaataaatg aacgcatttt tccagaccag actctgaaga
      121 aggtatttga ggaatttttt attagcaaac acagttgatt ttatagaaga ggatttgtac
      181 agtttttctg tacatatcct tccgggaaaa atgatttatg ccaattcaaa ccacctacac
      241 tgatgcccga gcgaattttg cgaagttgtg cgatcaggtg acatctgatc gtgaggtggc
      301 gatcattact cgccgtggtg ccgaagatgt tgccctgatt tcagcctctg aattatccag
      361 tttgcttgaa acggcacatt tgttgcgctc cccaaagaat gcggaccggt tattgactgc
      421 gctcaagcgt gctcaggagc ggacggtctc ttctcaaacc gttgatgaac tgcgccagga
      481 gcttgggatt gatgagtgat cgggatgctg tgttccagcc tgaatttcgc gaggatttgc
      541 agttttgggt cgaaacgaat cgcaaaaccg ctttacgagc atttgctttg attgaagcca
      601 ccatgcgcga tccgttcgaa ggcattggta aacccgaacc gctcaagtat ttgggggcga
      661 atgtctggtc acgccgactg acccaggaac accgcatcgt gtatctggtc agtgacgacc
      721 gaattgattt tctccaggcc agataccatt actgatgcgg atgaagaatt gagaaaagaa
      781 tgattgccct gcgaagcggg tggaaggcgg tttttttcct ttcattcgcc cagaaacttg
      841 tctaaacttc gtccctcctt gctaaatccc tccagataaa tgtcttataa gaaaattccg
      901 aaagtgtgaa tttgtgaaca taaaaataaa gcaaggggag ccctggtcgc tggcggggat
      961 gatcaacaat tttgaaacca tctgtgatac cgaaggccgc ccggtgtttg agggacgcgc
     1021 acccgttggg gtcaaagaga aagtgccgat ggtgatgcgc aagtgtccat accctgacga
     1081 acgccagggg cggatgatga atgtgtcagc cttaaaccag attaccgagc acctggatgc
     1141 cgctctggag aacatcgcca gctatcgcct cacgctgcct tcaacggtga caagctggga
     1201 ctttttttat cgcgctattc tcgaccagct tgggcaggcg gcgctctaca ttctgcgaaa
     1261 caaacccgtt ttaccggtcc agttgtcagt cacttacaaa gtcgcaaccg ggtatcaggt
     1321 gccgctccgc gaattgctca atctggagtt aagccgccag gcgccggtgc caacagttgc
     1381 aaacctgctc gattacgtca agcaacgtaa agcactggtc ggcaagcgcg aggtgtgtgc
     1441 ggccccaatg agcatggtca ccaaagtcac tgaagtgttg tatgacggat tgccagacca
     1501 gaaaacaaag gctcgccctg agcgtgtcgc cgtggcccag gcattgatgc tgcaaacctg
     1561 gttgggaatc gtctggcgac ggtttgacct cgcggcggag cgccagcttt tgctcgaaga
     1621 tctcgggcct gccacgctct ttccacggac tccgtacctg aagcggttgc tggctgatcg
     1681 cctgggcgag atcgaacaat ccgaggtcgt tgactacttt gaactgccac acttattgcc
     1741 cccagacctt cgccagtctc tgcgtcgcac gatggcgttc cttgaaggtg gtcaagaact
     1801 atgtggtgag actgtgtcgc tgatccagtc actgatggag catgatgatg ccgtggtggt
     1861 gatgtctgat ctgacgcggc gaccggtctt tgcccaacga tttgttgcct atctgaaagc
     1921 ctatcgctcg tttgtggcgc actttgaagc gattgaaatt gatctccgcc agcggcttgt
     1981 ttttccaact gaaccgccgg tgctgcttga tcccagtttt tttccttcac cacgggcgct
     2041 gaagtggttt gaactggtga gcgggcacct gattcgcccc aatcccgaag tgtctggcgg
     2101 gttgatgttt cagaatcagc accggagcat tccaattcca ttgataccag tcagtaaaaa
     2161 ccagggcttg gggtttgggg ctgaagaaaa cgggctgaag aaggcggatt gagggg
//
LOCUS       DAOQDO010001053         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46158_length_2215_cov_2.112037, whole genome shotgun sequence.
ACCESSION   DAOQDO010001053 DAOQDO010000000
VERSION     DAOQDO010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46158_length_2215_cov_2.112037"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKZ53_15735"
     CDS             <1..327
                     /locus_tag="PKZ53_15735"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNJ41941.1"
                     /translation="LGFFDIRVQVESMVRNHGSTAAVLVDTPEHERSYPSTWQDLRLD
                     RPVTEVEWLNGEIVRLGKQFGVPTPCNSVLREGVLASVRDHSGPGKYTLIQLLTAIED
                     EQRRSE"
     gene            complement(508..>2215)
                     /locus_tag="PKZ53_15740"
     CDS             complement(508..>2215)
                     /locus_tag="PKZ53_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41942.1"
                     /translation="NSGGGDSRYFSKPWYQAGLGVPDNEVRNVPDVSLLAGSPGYVYV
                     ISGNIGLIGGTSIAAPCWAGLCAILNEAQAASGGLGNINQELYRLGNAQARAQGPRVF
                     TDMLIGNNDQQSIRGFDAQPGYDRVSGWGSFVADALVEQFGRSPAAETVLPVISVVTP
                     TAGDPIESQQTIQIRWQSSDNLALMRHTIRLSSDGGKTFPTVVAAGLAGTAQTFDWQT
                     PPTTDARLDLQLAVTAIDTSFNQSTALSSVFSVTPDRTPPEVEILSPRAGANVKRFAS
                     HRVEWAASDNIALEAFDLSYIQAENGTVIELAAGLPGTARSFDWSPNLGFPLAVSSFI
                     KIQATDINHNTREARSDTFALTDDPPPLVQVISPTDLQEITIGQTTTIRWEVFQTTEP
                     LAVIHLQLMDFKTDESREIVTGLPGDSLSFDWTPLTDVRPTQSAFINLTASDISGSTR
                     STFSRGFVLVADTEPPAVTVLAPNGGEKLKPGKRATVTWTSSDDAGIAHQDLFLSLDS
                     GATYPIRLATGLPATAQSFVLEKNTIPAKSKSARLRVIATDHSGNQGQDDSDRDFQIK
                     GK"
BASE COUNT          557 a          569 c          571 g          518 t
ORIGIN      
        1 ctgggctttt tcgatattcg tgttcaggtt gaatcaatgg tgcgaaatca cggctcaacc
       61 gccgctgttt tggttgacac cccagaacac gaacggtcgt acccttcgac ctggcaggat
      121 ttgcgtctgg accgtccggt caccgaagtt gagtggctca acggcgaaat tgtgcggtta
      181 ggaaaacagt ttggtgtgcc aacgccctgc aattcggttt tacgcgaagg tgtcctggct
      241 tcagtccgcg accacagcgg cccaggcaaa tacactctga ttcaactgtt aacggcaatt
      301 gaagacgagc agcgaaggag tgaataagga atgaacacaa aataacctgg tcaactgaaa
      361 caaactctat cggaagtcga aaactcacca cagaggacac ggaggagcac agaggaaaat
      421 caatcaaccg aaccctctgt gttcctctgt gtcctctgtg gtgaaaaaat gatgaagtta
      481 tttaattttt atgcccaagt gaatttgcta ttttcctttg atctgaaaat ccctatcact
      541 gtcgtcctgc ccttgattcc ctgaatgatc ggtggcgatg actcgaagac gcgctgactt
      601 gcttttggcc ggaatcgtgt tcttttcgag cacaaacgac tgtgcggttg cgggcaaacc
      661 agtcgccagt cgaatcggat aggtggcacc gctgtccaaa gaaagaaaaa gatcctggtg
      721 ggcaatgccg gcatcatcag atgatgtcca ggtcacagtt gctcgcttac cgggtttgag
      781 tttttcacct ccatttgggg caagcactgt gacagcgggc ggttcagtat ccgccactag
      841 cacaaatcct cgcgagaatg tgctgcgtgt actgccgctg atgtcagaag cggtcaggtt
      901 gatgaacgca ctctgggtag gtctgacatc agttaacgga gtccagtcaa acgagagcga
      961 atcacccggc agcccggtca caatctctct tgattcatct gttttaaagt ccatcaattg
     1021 cagatgaatc accgccagcg gctcggtggt ttgaaacact tcccaacgaa tggtcgtcgt
     1081 ttggccaatt gtgatttcct gcaaatcagt aggtgagatt acctgcacca gaggaggtgg
     1141 gtcatcggtc aaagcaaatg tgtcactgcg agcttcacga gtgttgtggt taatatcggt
     1201 ggcttgaatt ttgatgaaac ttgaaaccgc cagtggaaac cccaaattcg gagaccagtc
     1261 aaaactccgg gcggttccag gaagtccagc agccagttca atgaccgttc cgttttctgc
     1321 ctggatgtag ctcaggtcaa acgcttccag tgcaatattg tcagaagctg cccattcaac
     1381 ccgatgtgag gcaaaacgtt tgacattggc accagcacgc ggcgaaagaa tctccacctc
     1441 gggaggggta cgatctggag ttaccgaaaa aaccgaactc agtgcggttg actggttaaa
     1501 actggtatca atcgctgtaa ctgccagttg aaggtcgagt cgagcatctg tcgtcggagg
     1561 tgtttgccag tcaaacgttt gagccgtccc agccagcccg gcagcaacca cggttggaaa
     1621 ggtttttcct ccatctgaag acaggcgaat cgtgtggcgc atcagcgcga gattatcgct
     1681 tgattgccag cgaatttgaa tggtttgttg tgactcaatt gggtctccgg cagttggtgt
     1741 gacgactgaa atcaccggca gcaccgtttc agccgccgga gatcgcccaa actgctcaac
     1801 cagcgcatca gccacaaacg aaccccaacc gctcacccga tcatatcctg gttgagcatc
     1861 aaaaccgcga atggattgct gatcattgtt accaatcagc atatcagtga acaccctggg
     1921 gccctgggca cgagcctggg cattccccaa ccgatagagt tcctggttaa tattcccaag
     1981 tccgccactt gcagcctgcg cctcatttaa gatggcgcac agtccggccc aacagggtgc
     2041 ggcaatgctg gtaccaccga tgagaccaat attgcctgaa atgacatata catatccggg
     2101 agaaccagcc aggagtgaaa catccggtac attgcggact tcgttgtcag gaacacccaa
     2161 tcctgcctga taccagggtt tggaaaaata gcgactgtct ccgccgccac tgttt
//
LOCUS       DAOQDO010001054         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46179_length_2214_cov_3.167670, whole genome shotgun sequence.
ACCESSION   DAOQDO010001054 DAOQDO010000000
VERSION     DAOQDO010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46179_length_2214_cov_3.167670"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..301)
                     /locus_tag="PKZ53_15745"
     CDS             complement(80..301)
                     /locus_tag="PKZ53_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41943.1"
                     /translation="MLNHPDVLAVALMKPRTFHICSAHQKARDVLKAGKIPADFTCPY
                     ASKTCPLLTILQLDPGKSLYLSGTGSPKL"
     gene            complement(355..1779)
                     /locus_tag="PKZ53_15750"
     CDS             complement(355..1779)
                     /locus_tag="PKZ53_15750"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ41944.1"
                     /translation="MSQCGVKFKANPTGEQKRILSEWMGCARFVYNAKVEEMDYFWTF
                     RQHNLALTGYSLPIDQTYSQFKTELTPWLSDCPSQILRNAVTKWYEAFQRFRQGKSGL
                     PVKKKKGKRDSILLTNELFRLDGVEDQETGKGTYKLFVGSEQNNLGYLSFEAHREFEF
                     PNSVTISRKNGDYFVSFSYEDGRKAPTEEELIGQYGQLDEATLGRITLGIDRGVVNPV
                     YTSDGEVFDFTQEQKASQAKADHKIEREQRKLARQKDKGSRRRERTKRKIAKAHSKKA
                     NIRRDFAHQTSRKLVDSDKQIFVVEDLKIANLTAAPEPKPADDGKTYLPNGAAAKAGL
                     NKSILQAGWGMLVTFVTYKAFRCHKLVLKVPPHHSSQECAECGHTHPDNRPSQSEFVC
                     GRCGHIDHADHNGARVVGKRGIKKLQKSVGVNPPSPPAGTRGSARRGTVRRRKESRPA
                     QPETPVSPKLERSANRPGEDRLPL"
     gene            1847..>2214
                     /gene="tnpA"
                     /locus_tag="PKZ53_15755"
     CDS             1847..>2214
                     /gene="tnpA"
                     /locus_tag="PKZ53_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNJ41945.1"
                     /translation="MIKSLSKDWRAGRTCVFQNHLHLVFVTKYRRDVLSDAMLIRLQE
                     LLTQTCLQLDCELLEFNGEHDHVHLLVSVHPKIAISNLVGKLKGKSAYFLRREFWPEM
                     KSKLWGNHFWGPSYCAVSCGG"
BASE COUNT          450 a          606 c          526 g          632 t
ORIGIN      
        1 gaagccccga atacactgaa ggttggtagt cagcaacgac cgtttcttcc gctggttgtg
       61 tctttggata attgaagaat cacaatttgg ggcttcctgt tccactgaga tacagtgatt
      121 tccctggatc cagttgcagg atggtcagca gtgggcaggt ttttgaagca tacgggcagg
      181 taaaatctgc cgggattttt ccagctttga gtacatcacg tgctttctgg tgagctgagc
      241 agatatgaaa ggtgcgtggt ttcatcagag caacggccag aacgtcagga tggttgagca
      301 aatagtgaac ctctcggctc tcaagagccg agcttccgtt tcgggccttc cgtctcacaa
      361 cggtagtctg tcttcccctg ggcggttcgc gcttctttcc agtttcggag aaactggagt
      421 ttcgggctgc gccggtcggc tttcctttcg gcgtctgact gtccctcgac gcgcactccc
      481 ccgcgtcccg gcgggtggtg acggtggatt tacacccacc gatttttgga gcttcttgat
      541 tcctcttttt cccacgactc gggcaccgtt gtggtcggcg tggtcaatgt gtccgcaccg
      601 accgcacaca aattcgcttt gtgacggtcg gttgtcggga tgagtgtggc cgcactcggc
      661 acactcttga cttgagtgat gaggaggaac tttgagaacc aacttatggc aacggaaggc
      721 tttgtaggtc acaaacgtga ccaacattcc ccaccccgct tgcaatatac ttttgtttaa
      781 cccggctttg gctgccgccc cgttcggcaa ataggttttg ccgtcgtcag ccggtttcgg
      841 ttccggagcg gcggtcagat tcgcaatctt taagtcctcg accacgaaga tctgcttgtc
      901 cgaatccacc agcttccggc tcgtctggtg cgcaaaatca cgccggatat tcgctttttt
      961 gctgtgtgcc ttggcgatct tgcgttttgt ccgttctctc cggcgtgagc ctttgtcttt
     1021 ttgacgcgcc agcttccgct gctctcgttc aatcttgtga tcggctttgg cctgggacgc
     1081 cttttgctct tgggtgaagt caaatacttc accatctgat gtatacaccg gattcaccac
     1141 cccacggtca attccaagcg tgatccggcc taaggtggcc tcgtccaatt gaccgtactg
     1201 cccaatcaat tcttcttcgg ttggcgcttt ccgcccgtct tcataggaaa acgaaacaaa
     1261 atagtccccg ttcttgcggc tgatggtgac tgagttcgga aactcgaatt cccggtgcgc
     1321 ctcgaaactc aaatatccaa gattgttttg ctccgagccg acaaacaact tgtatgtgcc
     1381 ctttccggtt tcctggtctt cgactccatc caatcgaaac agttcgttcg tgagcagaat
     1441 tgaatcgcgc ttgccttttt tcttcttcac tggcaacccc gatttgcctt gccgaaacct
     1501 ttgaaacgct tcgtaccact tggtgacagc attgcgcaga atttgggacg gacaatcgct
     1561 taaccacggt gtcaattccg tcttgaattg agaatacgtc tggtcaatcg gcaatgaata
     1621 ccccgtcaat gccagattat gctgccgaaa cgtccaaaag tagtccatct cttccacttt
     1681 ggcgttgtac acaaacctcg cgcaccccat ccactcggac aagatccgct tttgttcccc
     1741 ggttgggttc gctttaaatt tgactccgca ctgtgacata atctactcta aacacattta
     1801 atctacttgt ttccagtagt atacaatact ttttgagggc taggcaatga tcaaatctct
     1861 ctccaaagat tggcgggcag gccgcacctg cgttttccaa aaccacctcc acctcgtctt
     1921 tgtcaccaaa tatcgccgtg acgtcttatc cgatgccatg ctcattcgtc ttcaggaatt
     1981 gctgactcaa acctgcctcc aattggattg cgaactcctc gaatttaacg gtgagcacga
     2041 tcatgttcat cttctcgttt cggttcatcc caaaattgcc atttccaatc tggttgggaa
     2101 actcaagggc aagagcgctt attttcttcg ccgtgaattc tggcctgaaa tgaaatccaa
     2161 actttgggga aatcatttct ggggtccttc ttattgcgcc gtatcctgcg gtgg
//
LOCUS       DAOQDO010001055         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46214_length_2213_cov_3.104263, whole genome shotgun sequence.
ACCESSION   DAOQDO010001055 DAOQDO010000000
VERSION     DAOQDO010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46214_length_2213_cov_3.104263"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKZ53_15760"
     CDS             <1..322
                     /locus_tag="PKZ53_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41946.1"
                     /translation="VALGDWKSGMCLGSNGSNTPAYPASKLELAVAGNTEVIRAKKTA
                     AQSLGLNDHIEDILITLKTQYHLIRMVEAHPSLFFYLVLDRNKSNLAMARLKLAQIEQ
                     KISI"
     gene            complement(568..1707)
                     /locus_tag="PKZ53_15765"
     CDS             complement(568..1707)
                     /locus_tag="PKZ53_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ41947.1"
                     /translation="MKTVLIVDDERLFLSSLADVLPSYFEDLKVLTAEDGSEAIITLH
                     TEEVDLVVTDLKMPLMDGFQLLVYMLNKQINIPVVVMTAFGTPDIEQQVSAFGAVGWI
                     EKPIDTQVLADTIKECFLRQEGLNRRVRGNLKGITLATFLKILETESKTCTLRVISEG
                     RVGTIYFKAGELVNAEHELRRGEQALFEILNWHPEEITLENVCKKKDRVITRTLTQLL
                     RDLSNDEPPAPETLETAVSPTPEIPPQPVIEDNHDNGAQSYPWVNDLLDGLRSIDGLK
                     GLGLANWKQGTPLGVRNITHQCDQAELCQEYSKTVKIVVQSMRDLEPEGTVEDMLFTT
                     EDEFQLIRVVPTHPELFILVTIDKQRGNLAMARYELTGLLHKFGV"
     gene            complement(1869..>2213)
                     /locus_tag="PKZ53_15770"
     CDS             complement(1869..>2213)
                     /locus_tag="PKZ53_15770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ41948.1"
                     /translation="SLPFVKIDQRALHQILMNLFSNSADALEGSEDPKIKISAFVKDK
                     RVWIEIEDNGVGMIEEQVLNLFKPFHTSKPHGTGLGLVITKRMVTRMNGTITVTSRPS
                     VGTTVGLSFSPA"
BASE COUNT          514 a          598 c          524 g          577 t
ORIGIN      
        1 cgtcgcgtta ggcgattgga aaagcggcat gtgtttaggc tcaaatggct cgaatacccc
       61 agcctatccg gcttcaaaac ttgaattagc ggttgcaggg aataccgaag tgatccgggc
      121 caagaaaaca gccgcccaat cacttggctt gaacgaccac attgaagaca ttttgattac
      181 tcttaaaacc caataccact tgattcgaat ggtcgaagct catccatcac ttttctttta
      241 tctggtttta gatcgaaaca aatcaaatct ggcgatggct cggctcaagc tggcacaaat
      301 cgagcagaag atctccattt aaaaccacgc gggcttgggg ctgaaaactt tgggctgaag
      361 aagtcggggt cagggtatcc aacccgtttt cttcagccca gcttcttcag cccgctttct
      421 tcagcccgct ttcttcagcc cgctttcttc agcccccagc ccaacttctt cagcccgctg
      481 tcttcagccc caagcccgat gtcttcagcc cacttttttc agcccccagc ccaacttctt
      541 cagcccgctg tcttcagccc cacatcatta caccccaaac ttgtgcaaca agccggtgag
      601 ttcataccgg gccatggcga ggttgccccg ttgcttatca atcgtgacaa gaatgaataa
      661 ttcgggatga gttggtacga cccggataag ttgaaactca tcctcggtgg taaataacat
      721 atcttcaact gtcccctcgg gttcaagatc cctcattgac tgaacaacga tttttacagt
      781 tttgctatat tcctgacata gctctgcctg atcgcactgg tgggtgatgt tgcgaactcc
      841 gaggggggtt ccttgcttcc aatttgccag acccagtcct ttcaaaccgt caatgcttct
      901 gagcccgtcg agcaggtcat tgacccaggg ataagattgt gcgccattgt catgattgtc
      961 ctcgatgaca ggctggggag gtatttccgg ggttggtgaa accgccgttt ccagggtttc
     1021 cggtgcagga ggttcatcat ttgaaagatc ccgcaataat tgagtgagcg tgcgcgtaat
     1081 gacccggtct tttttcttac agacattttc caaggtaatt tcttccgggt gccagttcag
     1141 gatttcaaac agggcctgct caccccggcg gagttcatgt tcggcgttaa ccagttcacc
     1201 ggctttgaaa taaatcgtcc ccactcgccc ttctgaaatg acccgcaggg tgcaggtttt
     1261 gctctcagtt tcaagaattt tcagaaatgt cgccagggta attcctttga gattgccccg
     1321 gactcgccgg ttgagtcctt cctgcctgag aaaacattct ttaatcgtat cggccagcac
     1381 ctgagtatca attggttttt caatccaccc aaccgcccca aacgccgata cctgttgttc
     1441 aatatccgga gtcccaaacg cggtcatcac cacgaccggg atattgattt gtttgttcag
     1501 catatagacc aggagttgaa acccgtccat cagcggcatt ttgaggtctg tcacgaccag
     1561 atcaacttct tctgtgtgca gggtgataat tgcctctgag ccatcttcgg cggtgaggac
     1621 tttcagatct tcaaagtaag agggcagaac atcggccagg ctgcttagaa acaagcgttc
     1681 atcatcaacg attagaactg ttttcataag aatgtcttct ctccagagtc aagtagaata
     1741 gctgggcctt gggctgaggg cttggggcca taagcaccag gatcaggatt tcaggcccgc
     1801 aggggtcgcc gttcaatagc cgtggtgcga agcccacggt cacgatcagc acgagaccca
     1861 ggcccatctc acgccggtga aaaactcaag ccaaccgtgg tgccaacact tggccggctg
     1921 gtgacggtga tggtcccgtt catgcgcgtc accatccgtt tggtaatcac cagtcccaac
     1981 ccggtgccgt gcggtttgga ggtgtgaaac ggcttaaaga gattgagcac ctgctcttcg
     2041 atcatgccca ccccattgtc ctcgatttca atccaaaccc gtttatcttt aacgaatgcg
     2101 gaaattttga tttttgggtc ctccgatccc tccagggcat cggctgaatt tgaaaacaga
     2161 ttcatcagaa tctgatgtag agcgcgttga tcgattttga caaatggcag gct
//
LOCUS       DAOQDO010001056         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46222_length_2213_cov_2.690454, whole genome shotgun sequence.
ACCESSION   DAOQDO010001056 DAOQDO010000000
VERSION     DAOQDO010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46222_length_2213_cov_2.690454"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..>2213
                     /locus_tag="PKZ53_15775"
     CDS             461..>2213
                     /locus_tag="PKZ53_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41949.1"
                     /translation="MSKRFLFIMLVLTVLFGGLMFDLSLSSENVQAGNSITVTDKPDA
                     DPTPYPVVEMAGQIKREAAYFSTSGSDQLSNASHLAHNREVVPSQAVGSMVFSLGFLA
                     GGLLLAVLLVSFPGTGARAGVLLCGKWGAAMCVLAALVGVRGVVGVPGTTRTIPKPMP
                     APVKQTGSGAPDFVASLEIGGRGTTQIGGTAVDEAGNVYVTGGFSDHIFFATTPPTKL
                     TATRGYDFFVAKYSPDRKCLWARMANGTSDIPADLSLDGGLTITVDSQGNCYVGGGFV
                     SSLAFQTESGTTAVTLAASGSGLNAEAFVAKYNSDGTLLWARGGNSNSPQSTDSLNTS
                     VNSILKIVVDRQGTPYVTGQLAGNSFLGKQVQISGAQDSILARLNPATGDPVWTAAPV
                     STGSEDIFDLAVDGVGNLYLVGAFLGQPITFPTPSSPTTFALSDQDTDTYIAKYTPSG
                     QCVFARQIGGGRIVGFDIAATSTGDFYVTGAFISHAVFGDVTLTSNTVSISGFLAKYS
                     TTGSLLFAQDFGKNLYTSGNRVEVDAGGTVYVVGYLRGEAIFGGIGQNAQTLSSSGVS
                     MFLAVYNAAGAFEYARLL"
BASE COUNT          481 a          552 c          661 g          519 t
ORIGIN      
        1 tcccaaaatt tgtgtctggc tgacactggc cgtggttctc ggtgcatttc gaggccgggc
       61 tgaagaagac gggctgaaga agccgggcta ggggctgagg gttgaagaaa gtgggttgaa
      121 aaagccgggc taggggctga gggttgaaga aagtgggctg aaaaagccgg gctaggggct
      181 gagggctgaa gaaagtgggc tgaaaaagcc gggctagggg ctgagggctg aagaaaacgg
      241 gctgaagaag ccgggctagg ggctgagggc tgaagattcg caagctcagg gcaatcgaag
      301 ggatgaagga tgaaggatga gggatgaaat aaaaccaact cttcggccca acttcttcag
      361 cccgatgtct tcagcccgac ttcttcagcc cgaagtcttc aacccgaaga atcggtcgcc
      421 aatgtcccac cccattcctt aatgtcccag gagaaagact atgtccaaac gattcctttt
      481 cataatgctc gtcctcactg tgctcttcgg agggctgatg tttgatctgt cgctgtcatc
      541 agagaacgtt caggcaggca actcaataac agtcactgac aaacctgatg ctgaccctac
      601 gccttaccct gtcgtggaga tggcaggtca gatcaaacgg gaagccgcct atttttcgac
      661 ttccggctca gatcaactct ccaacgcatc ccatttggct cacaaccggg aggttgtccc
      721 atcgcaggcc gttggttcca tggttttttc gctggggttt ctggctggcg gactactcct
      781 ggcggttttg ctggtttctt ttcctggaac gggcgccagg gctggcgtgc tgttgtgtgg
      841 gaagtggggg gctgccatgt gtgtgctggc agcgctggtg ggcgttcgcg gcgtggtcgg
      901 tgtgcctggt acgacacgca ccattccaaa accaatgccg gcacccgtga aacaaactgg
      961 aagcggagcc ccggattttg tcgcttcgct ggaaatcggc ggacgtggga ccactcaaat
     1021 tggtggaact gccgtggatg aagccggcaa tgtgtatgtc accgggggtt tttcggacca
     1081 catctttttt gccacaacac cgcccacgaa attgactgcc accagaggtt atgatttctt
     1141 cgtggccaaa tacagcccgg accgaaaatg tctatgggcc cgcatggcaa atggaacgag
     1201 tgatattcct gcggacctgt cgctggatgg tgggctgacc atcaccgttg atagtcaggg
     1261 gaattgttat gtcggtggcg ggtttgtttc atcgctggca tttcaaaccg aaagcggtac
     1321 cacggcagtg accctggcgg cttccggctc ggggctcaac gccgaagctt tcgtggcgaa
     1381 atacaactct gacggcactc tgttgtgggc gcgaggtggg aacagcaaca gccctcagtc
     1441 aacggattcg ttaaatacca gtgtcaacag catcctcaag attgtggttg atcgtcaggg
     1501 aacaccctat gtgactggcc aactggccgg aaattcgttc cttggaaagc aagttcagat
     1561 ttccggcgcc caggattcga tcctggctcg gctcaatcca gcgactggcg atccggtttg
     1621 gaccgcggcc ccagtttcga ctggttcaga agacatcttt gatctggcag ttgatggagt
     1681 ggggaatctg tatctggtcg gagctttttt aggacaacca atcacgtttc caaccccctc
     1741 gtcaccaacc acctttgcct tgagtgacca ggataccgac acctatatcg cgaaatacac
     1801 tccgagtggc cagtgtgtgt ttgcacgaca gatcggcgga ggccggattg ttgggtttga
     1861 cattgccgct accagcactg gtgattttta tgtcaccgga gcttttatct cacacgccgt
     1921 gtttggtgac gttaccctga cgagcaatac agtcagtatc tcagggtttt tagcgaagta
     1981 cagtacaact ggcagcttgt tgtttgccca ggactttgga aaaaaccttt ataccagtgg
     2041 aaaccgggtt gaagtggatg ctggtgggac tgtctacgtg gtcggatatc tcagaggcga
     2101 agcgatcttt ggcggtatcg gtcaaaatgc ccagaccctc agctccagcg gggtcagcat
     2161 gtttctggcg gtctacaacg cggcgggggc ctttgaatat gcccggctgc tcc
//
LOCUS       DAOQDO010001057         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46249_length_2212_cov_3.042188, whole genome shotgun sequence.
ACCESSION   DAOQDO010001057 DAOQDO010000000
VERSION     DAOQDO010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46249_length_2212_cov_3.042188"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..362)
                     /locus_tag="PKZ53_15780"
     tRNA            complement(290..362)
                     /locus_tag="PKZ53_15780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Lys,seq:ttt)
     gene            594..666
                     /locus_tag="PKZ53_15785"
     tRNA            594..666
                     /locus_tag="PKZ53_15785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:627..629,aa:Thr,seq:cgt)
     gene            891..>2212
                     /locus_tag="PKZ53_15790"
     CDS             891..>2212
                     /locus_tag="PKZ53_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41950.1"
                     /translation="MKQCSACNREWNDQMVFCPIDGNVLSLGVLLNRKYLLEDKIGEG
                     GMGIVYRARHTRLDTIVAVKVLHPRLTPDEAAVERFLREARAAAKINHDNAIKVSDFD
                     TADGIAYFVMEYLDGAPLSERIARGRLSYPETALILRQICAALEKAHSRGILHRDLKP
                     DNIYLTVDEDGKQLVKVLDFGIAKFFSSEGNSLTAEGMIVGTPKYMSPEQGLDQPLDS
                     RSDIYSLGVILYEMLTGQVPFKASTPVATLLKHVHDTPQPLRELCPDILPAVEAVVLR
                     ALAKNRVDRPGSAKQLAREFDEALSMSAKTVESHPLTSPPVPPVTVRVGRSQPQPPEI
                     FPPTTLAVESAPAISPEAAPAVLPQTPTPKTQPRLSTPTQADQENWSAVGLVTDSASA
                     EPTPVPVEAAVSTAPTLTRQTDSLDRFKPWRMVSWVGGGGLVILGILFG"
BASE COUNT          506 a          562 c          562 g          582 t
ORIGIN      
        1 ccgagatacg gctgaagctg atcggcggat ggttcgggag atggttcagg atttgaagga
       61 cacgatttac cgggttaaat ccaaagccga taaagcgttc atcctggcaa ggcaggattc
      121 agaggacaat cgctgatgag gctcaaagaa tgatgctgca gcctgacttc aagacaattt
      181 gggcaaaaaa agaggtggat ttttgaagat ccaccttttt ggtatccaat tttgtgtcca
      241 aacccaactt agaggatggt gccatcccct aagttgttga ttttattgat gagccgtgta
      301 ggactcgaac ctacgaccca ctggttaaaa gccagttgct ctacctcctg agctaacggc
      361 ccgcaagaaa cctgcagaag ttggccagta taggaatcaa ccccggactc gtcaagccac
      421 ttttttaaag tttttgccgg attttttggc aagctctttg ttaagaagaa cttatcctta
      481 attcatcagg cacaggccag ttttttgcat tgatggcaca atatccttta agaagggact
      541 tgacagccgt gttatactgc gcgttcactt ttcaatacac ctgcctgcag taagccgact
      601 tagctcagtt ggtagagcga ctgattcgta atcagtaggt caccggttca agtccggtag
      661 tcggcttcat tcctttttag atccacctgc ttttgccact tcttcctgcg ctcagtttct
      721 tccctcgctg aatttcttct attggccagg ccacgccgga ggttacaagt cattgggtat
      781 ctgtctgttt ccttgaatca gatgcctgag ctatgtaaca tttgccttcc tctttttgtc
      841 ccaatcctca cacgtttatt tggctaaact ccaggtttcg aggcaacggc atgaaacaat
      901 gttcggcgtg caaccgagag tggaacgacc agatggtgtt ttgtccaatt gatggcaacg
      961 ttctttcgct cggtgttttg ctcaatcgga aatacctcct tgaagataaa atcggtgaag
     1021 gcggaatggg gattgtgtat cgtgcccgac atacccggct tgacaccatt gtggcagtca
     1081 aggtgcttca tccacgtctc acaccggatg aagccgcggt tgaacgtttc ctgcgtgaag
     1141 cccgtgccgc agcgaaaatt aatcatgata acgcgattaa ggtttctgat tttgataccg
     1201 ctgatggtat tgcctatttt gtgatggaat acctcgacgg agccccattg agtgaacgaa
     1261 ttgctcgtgg tcggctttcg tatccggaaa ccgctttgat cttgcgccag atttgtgcgg
     1321 cgctggaaaa agctcactcg cgtgggattc tgcatcgtga tctgaaacca gacaatattt
     1381 acctgacggt tgatgaagat gggaaacaac tggtcaaggt ccttgatttt ggcatcgcca
     1441 agtttttttc ctcggaaggg aattcgctga cggcggaagg catgattgtc ggcaccccaa
     1501 agtatatgtc acctgagcag gggcttgatc agccgctgga tagtcgttcg gatatctaca
     1561 gtctgggtgt cattttgtat gaaatgttga ctggccaggt cccatttaag gcttcaactc
     1621 cggtggcaac gcttctcaaa cacgttcacg acacgccaca accgctccgg gaattgtgcc
     1681 ctgacattct cccagccgtt gaagcggttg ttttgcgagc actggccaaa aatcgagttg
     1741 atcgcccagg atcggcgaaa caactggcca gggaatttga cgaagcgctt tcaatgtccg
     1801 cgaaaacagt cgaatctcac ccgttgacat caccgcccgt gccaccagtc acggtgcgtg
     1861 tgggccggtc tcagcctcag ccgcctgaga tttttccgcc caccacactg gctgtggaat
     1921 ctgcgcccgc catttcgcca gaggcagcac cggctgtact gccacaaacc ccaactccaa
     1981 aaactcagcc tcgtctcagt acgccaactc aggccgacca ggaaaactgg tcggcagtcg
     2041 gattggtgac tgattcagcc tcggctgaac ccacaccggt tccggttgag gcagccgttt
     2101 cgacggctcc gacgctgacc aggcaaactg actcccttga ccggttcaaa ccctggcgga
     2161 tggtttcctg ggttgggggt ggcggcctgg tgattcttgg aatcttgttt gg
//
LOCUS       DAOQDO010001058         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46299_length_2211_cov_2.813080, whole genome shotgun sequence.
ACCESSION   DAOQDO010001058 DAOQDO010000000
VERSION     DAOQDO010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46299_length_2211_cov_2.813080"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKZ53_15795"
     CDS             complement(<1..576)
                     /locus_tag="PKZ53_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41951.1"
                     /translation="MMHPHNLSKRLGLSCFLGLVLALNPFFICVAVAQSVQPSENLEP
                     GKAVERELKGGEKHTYLTTLKANDYVKLVVEQKGADVVIRLIGPDGKTIQEVNSLYIK
                     QGQKQLSSITSQPGRYAVEIESLVKTSPPGKYELKLAVAKTATELDRAEMEIETLLGE
                     EAKLTQAGKFDQALPIAEKAVATSETAFGPDH"
     gene            complement(704..>2211)
                     /locus_tag="PKZ53_15800"
     CDS             complement(704..>2211)
                     /locus_tag="PKZ53_15800"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ41952.1"
                     /translation="NDDDIPATSAELTDISAVALDASGNLLIVDTSSNLLRRVDAATG
                     RISTLAGDGSGSFSGDGGPSQNATLDFFLFGGVAVDSAGNIFLADSGNNRIRRIDGRT
                     GIITTVAGNGLEKFGGDGGPAINASLGSPRGVFVDANDNLLIVDTFNNRIRRLDTKTG
                     VITTVAGNGVVGYGGDGGSAVSASLAKPNGIVIDKSGNLLIGDTFNNCVRAVKGFTKL
                     GTVVDFGVTASPFSQTVASGESASFTVMVPGINGFSQPVGLSAAINPPNPNLSVRFSN
                     GTVAPGETVTLAVSTTSSTPTGAYAVKVTGTASSRIHSQTVLVNVISTNSAPGLAPIT
                     DQTVQAGEVRMIPVSAFDPDGNSGLTLSLAASPTYVTLTDSGNGVGTIRMAPTASETQ
                     SGQVVVQVKDSGGLVAQTSFRVTVQAGSGVFITNATYTSPQLVITGSGFGASGAMVTI
                     NGQDVSKFIKSQNATTITLKGKPKKLKLQSGANQIIVTIGGVKSNTFVLTI"
BASE COUNT          496 a          596 c          578 g          541 t
ORIGIN      
        1 gtggtcaggc ccgaaggcag tttcactggt tgccaccgct ttttcggcaa tcggcagtgc
       61 ctgatcgaat tttccagctt gagtcagttt cgcctcttcg cccaaaaggg tttcaatttc
      121 catttctgcc cgatccagtt cggtggcagt tttggcgacg gccagtttca attcatactt
      181 tccaggtgga ctggttttca ccagagactc aatttcaacc gcataccggc ctggctggct
      241 ggtaatcgat gaaagttgtt tttggccttg tttgatataa agactgttca cttcctggat
      301 cgttttccca tccggcccaa tcaaccggat aaccacatcc gcgccctttt gttcaacgac
      361 caatttcaca tagtcattgg ctttcagtgt ggtcaggtag gtatgttttt cgccgccttt
      421 gagttcccgc tcaactgctt ttccaggttc caggttttct gaaggctgaa cactctgcgc
      481 cacagccaca cagatgaaaa aggggttaag cgccaatacc agtcccaaaa agcagcttaa
      541 acccagtctt ttgctcaaat tatgaggatg catcattttg acctccagtg gtagtgggga
      601 agggagggaa aagacaaagg caaagggtca gcctttcagg aggctggtac aaagcggaag
      661 ctgtcggtga cagacctgac gcaatgtatc taccaaatga ggactaaatt gtcaggacaa
      721 aagtgtttga tttgactccg ccaatcgtca cgatgatttg gttggcgcca gattgaagct
      781 tgagtttttt cggtttcccc ttgagcgtga tggtggttgc gttctgactt ttgatgaatt
      841 tgctcacatc ctgaccgttt atcgttacca ttgcccctga cgcgccaaat ccacttccgg
      901 taatcaccaa ctggggcgac gtatacgtgg cgttggtaat aaagaccccg cttccagcct
      961 gaaccgttac ccggaatgaa gtttgggcga ccagcccgcc ggaatctttc acctgcacca
     1021 ccacctgccc gctttgtgtt tcgctggcgg tgggtgccat ccgaatggtt cccacaccat
     1081 tgccgctatc ggtgagcgtg acataggtcg gggaagccgc cagagatagc gtcagtccag
     1141 aatttccgtc agggtcgaag gctgagacgg ggatcatgcg tacctcgccg gcctggacag
     1201 tctgatcggt aattggggct aaacctggcg ccgaattcgt ggaaatcaca ttcacgagca
     1261 ccgtctggct gtggattcgg gaactggccg tacctgttac cttcacggca taggcgccag
     1321 ttggagttga actggtggtc gaaaccgcca aggtgaccgt ctcgccggga gccaccgttc
     1381 catttgaaaa acgtaccgac agattgggat ttggcggatt gatcgctgca ctcaatccga
     1441 ccggttggga aaatccattg atcccaggta ccatcaccgt gaaggaagct gattctccgc
     1501 tcgccacggt ttgcgaaaac ggactggcag ttactccaaa gtccaccacc gtaccgagtt
     1561 tggtaaatcc tttcacggcc cggacacagt tgttaaacgt gtctccgatc aacaaattgc
     1621 ctgatttgtc aatgacgatg ccattgggtt tggcaaggct ggcactgact gccgagcctc
     1681 catcaccgcc gtatccgaca accccgtttc cagcaaccgt agtaatgact ccggttttgg
     1741 tatccagtcg ccggatgcga ttgttaaagg tatccacaat caagaggttg tcgttggcgt
     1801 caacaaacac tccccgtggt gaaccaaggc tggcattgat ggccggcccg ccatcgccac
     1861 caaatttttc caggccattt ccggcgaccg tcgtgatgat tccagttcga ccgtcaatgc
     1921 gccgaattcg gttattgccc gaatccgcaa gaaagatgtt tccggcggaa tcaacggcga
     1981 ctcccccaaa gaggaaaaag tcgagtgtgg cattttgcga tgggccacca tctccggaaa
     2041 acgacccgct tccatcgccg gcaagggtgg agattcgccc cgtggcggca tcaacccgcc
     2101 ggagaagatt tgaggacgta tccacaatca agagattgcc ggaggcatcg agggccacgg
     2161 ctgaaatatc cgtcaactcg gcactggtcg ccgggatatc gtcgtcgttg t
//
LOCUS       DAOQDO010001059         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46331_length_2210_cov_3.315081, whole genome shotgun sequence.
ACCESSION   DAOQDO010001059 DAOQDO010000000
VERSION     DAOQDO010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46331_length_2210_cov_3.315081"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKZ53_15805"
     CDS             <1..606
                     /locus_tag="PKZ53_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41953.1"
                     /translation="PAPAPIGPVPQQPAPSASSGRSLRWILAGIGGGLILLIGLLLIL
                     FAAYSKLAGTESAPSPAQSGHLPESPAPAPEPFPDRTGGAEGPVPTENPGRTPETNDP
                     GQILTEVKETIFKLKQQIASPAQTSASRAELTDHLIRLENSEKQLRQLMDNQSINLEA
                     KQQTIKQIHTALESAKKAISKVSAGETSFPDRKGTPSTNPS"
     gene            693..905
                     /locus_tag="PKZ53_15810"
     CDS             693..905
                     /locus_tag="PKZ53_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41954.1"
                     /translation="MNSRLLRFFSFALLFVLVNGMAAVFSAPRQSPTQTPVMSEAFKN
                     LLGTDDGAALAILYGSDTRGNIDTCG"
     gene            918..1805
                     /locus_tag="PKZ53_15815"
     CDS             918..1805
                     /locus_tag="PKZ53_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41955.1"
                     /translation="MGGLARRVSYTQSFRKLFPTTPVLELNAGYYLNDVGITNTPPLK
                     DIETQNLYAMHGYSQFPFDAANITFHDLPYLSRYLTKDQQKTAMAEFPGLKTMISANL
                     TPETGAQSHSPVVPYVIREIKSPRVPKGKIKVGIVGLSEPAPAAQFGYVWRDPDKAAR
                     EVLPELRKKVNLLIVLAYMPVDKAKLLATNCQEADVVISAFGQPYQSPLETVGNVSFV
                     TSFSETKFLGELRIKFDTAGKPTFVNRYVALDSFVPDDAQAASVVAEAKTAIEKARQE
                     MVEEQIKRARQQQPSSGGH"
     gene            1955..>2210
                     /locus_tag="PKZ53_15820"
     CDS             1955..>2210
                     /locus_tag="PKZ53_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41956.1"
                     /translation="MISRFFRFSVLAFLCVSLGLGGYLAPRSLRAQTQAPPQSDAFKN
                     LLGTDDGAGLAIFYGGDTRGKIEDCGCRAHPMGGLARLTGY"
BASE COUNT          565 a          621 c          519 g          505 t
ORIGIN      
        1 ccagcaccag caccgatagg ccctgtgccc caacaaccag caccgagtgc ctcatcaggt
       61 cggtctctac ggtggatact ggcaggcatc ggcggtggat tgattctctt gattggtctg
      121 ttgctcattt tatttgcggc ttattcgaaa ctggccggaa cagaatcagc cccctcccca
      181 gcccaatctg gtcacttacc tgaatcaccc gctccggccc cagagccatt cccagatcgg
      241 acgggcggcg ccgaaggacc agtccccacg gaaaaccctg gacgcacacc ggaaaccaac
      301 gatccaggac aaatcctgac tgaagtcaag gaaaccattt ttaaactcaa gcaacaaatt
      361 gcttcacccg ctcagacttc tgcctcgcgg gctgaactca ccgaccattt gatccggttg
      421 gaaaatagtg aaaagcaact cagacaattg atggacaacc agagcatcaa tctggaagcc
      481 aaacaacaaa ccatcaagca aattcacacc gctttagaaa gcgccaaaaa agcaatttcc
      541 aaagtatcag caggggaaac cagttttcca gatcggaagg ggaccccttc gaccaaccca
      601 tcgtaacccc ggcttgcgaa gagacctgcg agggaaactc cggaattgga gccccatcta
      661 cctctatcct ttacaacaag gcagtatctg tcatgaattc acgtttactt cgattttttt
      721 cgttcgcact gttgtttgtt ctggtgaacg gcatggcggc ggtgttttca gccccccgcc
      781 agtcaccgac ccaaacgccg gtgatgtcag aagccttcaa aaatctgctt ggtaccgatg
      841 acggtgccgc actggccatc ctctatggaa gtgatactcg gggcaatatc gacacctgtg
      901 gttgacgggc ccatcccatg gggggtctgg cgcggcgcgt cagttacaca cagtcttttc
      961 gaaaattgtt cccgacaact ccggtcctgg aattgaacgc tggctattat ctcaacgatg
     1021 ttgggatcac caacacgcca ccgctcaagg acatcgaaac ccaaaacctc tatgccatgc
     1081 atgggtatag ccagtttccc ttcgatgccg ccaatatcac attccacgat ctgccatacc
     1141 tgagccgata tttgaccaaa gatcagcaaa aaacggctat ggctgaattc ccgggtctca
     1201 aaaccatgat ttcagccaac ctgacacccg aaactggtgc ccaaagtcac agcccggtgg
     1261 tgccctatgt tattcgggaa atcaaaagcc cacgcgttcc caaaggaaag atcaaagtgg
     1321 gcatcgttgg cctgagtgaa cccgcaccag ccgcccaatt tgggtatgtc tggcgggatc
     1381 cggacaaagc agcccgtgaa gtccttcccg aactgcgcaa gaaagtcaat ttgttaatcg
     1441 tgctggcgta tatgccggtt gataaagcca aattgctggc gaccaattgc caggaagccg
     1501 atgtggtgat ctcggcattt ggtcaaccct atcaatcccc gcttgaaacg gtcggcaatg
     1561 tttcatttgt caccagtttt agcgaaacca aattcctggg tgaactccga atcaaatttg
     1621 atacagccgg caaaccaacc tttgtgaacc ggtatgtcgc gctcgactca tttgttcctg
     1681 acgacgctca ggcggcttca gttgttgccg aagccaaaac tgccattgaa aaagcccggc
     1741 aggaaatggt ggaagaacaa atcaaacgag ccaggcaaca acaaccgtca tccggtggac
     1801 actaaatcag ccaccatcaa tcacaaatct cttgattgag gtacttgagt gttgcggagg
     1861 cggtttttga gtttggttca gcattgaatg tcaccagatt tccaaaacca acgacccaat
     1921 ttcaacctac ctcaatcaaa aggtttacgt gcttatgatt tcgcgttttt ttcggttttc
     1981 tgtccttgca tttctgtgtg tttcacttgg cctcggtggc tatctggccc cacgttcatt
     2041 gcgtgcccaa acccaggctc caccccaatc tgatgccttc aaaaatttac tgggtacgga
     2101 tgacggtgcc ggtctggcca ttttttatgg tggcgacacc cgaggcaaaa ttgaagattg
     2161 cggttgtcgg gcgcatccga tgggcggact ggcccgactg acagggtatg
//
LOCUS       DAOQDO010001060         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46332_length_2210_cov_3.309513, whole genome shotgun sequence.
ACCESSION   DAOQDO010001060 DAOQDO010000000
VERSION     DAOQDO010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46332_length_2210_cov_3.309513"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKZ53_15825"
     CDS             <1..240
                     /locus_tag="PKZ53_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41957.1"
                     /translation="RCRCFIPKFSEISRIHIFGIYLAPGFWFGIQIAHREADSTYSRF
                     FIPLDTGTQHLHSLVFREEKNGATVHPDSTDPQEK"
     gene            356..>2210
                     /locus_tag="PKZ53_15830"
     CDS             356..>2210
                     /locus_tag="PKZ53_15830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ41958.1"
                     /translation="MSKQCFWLAPFFLLLSFIPVFGQATDGAMNGKVLDEQGASIAGA
                     AVKLTNMDTGFTSTVTSDANGLFRASYLPVGKYSITAEAAGFKTTSLEGIAVVLNQIV
                     DVTIRLQTGEVSEVVVVSSGDVLVETTTSTLGNTFESRKVVELPSLNNSQLELALLAP
                     NITSQAGGTAGEGGSVGGNRPRNNSFTLDGVDNNDTILTGHVIDVIPEAVAEFTLLTN
                     QYTAEFGHSSAGQFNTITKSGTNEFHGGASWFNINKNYNAVDHLTKEAISRGEIPDKK
                     PRFDFNRVAGYLGGPIIKNKLFFFGAYQYDTTGTAGAPVSFLTPTQEGFARLGTLNGV
                     SSTTLGILRQYTVPAGSASDTVTVLGQQIPVGTVNFLNANFEANHTFNVNVDQVLNNG
                     DQIRYRYNYAKQTAPNNGDGNPVFNGSFTQLNQLGSVAYIHSFTPTLINEFRVAYRRK
                     NNSFGIPSEFANFPNFAFDDLGYTFGPQGESPQGEITNSYQYVDNLTWIKGKHNLKFG
                     VEFRNVISANGFLPRGRGEYDYATLEQFLRDVKPTGFNGGLKGVGTSAFAANRKSIYW
                     YVQDDIKVTPTFTLNVGLRYEFYSLFRDEKLQALNAIASVPGVIEFGVPKSD"
BASE COUNT          565 a          554 c          533 g          558 t
ORIGIN      
        1 cgttgtcgct gtttcatccc taaattttct gaaatcagcc gtatacatat ttttggaatt
       61 tatttggctc ctggattttg gtttggtatt caaattgcac atagggaagc agactcaacg
      121 tactcccgtt ttttcattcc tttggatact ggcactcaac acttgcacag tttagttttt
      181 cgggaagaaa aaaacggggc cacagtacac cctgactcaa ctgatccaca ggaaaagtga
      241 agagagactg aaaaaaagcc ccggtgaagc attgttgcca tatcttgggc cagtgagaca
      301 agacgtcttt tcggcttttc cgcagaattt ttttaggaga gaaacggagg aacctatgtc
      361 aaaacaatgc ttctggctgg cgcctttctt tctgttgctg tcattcattc cggtgtttgg
      421 gcaggcaaca gacggtgcca tgaacggaaa ggtattggac gaacagggtg cttcgattgc
      481 gggagccgcg gtcaaattga ccaatatgga taccggcttc acatcgacag ttacctcaga
      541 tgccaacggg ctatttcgcg ccagctacct tccagtcgga aaatattcaa tcacggctga
      601 agccgctgga ttcaaaacca cttccttgga aggaattgcc gtggttttga accagatcgt
      661 tgacgttacc atccggttac aaaccggtga ggtcagcgaa gttgtggtcg tttcaagtgg
      721 cgatgtgctg gttgaaacca caacctcaac ccttggcaat acctttgaat cacgcaaagt
      781 cgtggaactg ccaagcctga ataactcaca gcttgagctg gcactgctgg caccaaacat
      841 cacgtcacag gctggcggga cggctggtga aggtggctca gtcggcggta accgtccgcg
      901 aaacaacagc tttacgctgg atggtgtgga caacaacgac accattttga ccggtcacgt
      961 cattgacgtg attccagaag cggtggcgga atttacgctg ttgaccaatc agtacacggc
     1021 tgaatttgga cactcgtcag ccggtcagtt caatacgatt accaaatccg gcaccaacga
     1081 atttcatggt ggtgcgagct ggttcaacat caacaaaaac tacaatgcgg ttgaccacct
     1141 caccaaagaa gccatttcgc gtggcgaaat ccccgacaaa aaaccacgct ttgactttaa
     1201 tcgcgtggcg ggttatttgg gtggtccgat catcaagaac aaattgttct tctttggcgc
     1261 ctatcagtat gacaccactg gtacggctgg tgcgccagtc agcttcctga cgccaactca
     1321 ggaaggcttt gcccgcttag gcaccttgaa cggtgtgagt tcaaccacac tgggcattct
     1381 tcgccagtac acggtcccag ccggttcagc ttctgacact gtcaccgtgt tgggccagca
     1441 gattccagtg ggaaccgtca acttcctcaa cgccaacttt gaagccaatc ataccttcaa
     1501 cgtcaacgtt gaccaggtgt tgaacaatgg cgaccagatc cgctatcgct ataactatgc
     1561 caagcaaacc gcgccaaaca atggcgatgg caatccggtt ttcaacggtt cgttcacaca
     1621 attgaaccag ttgggttcag tggcttatat ccacagcttc accccaacct tgatcaatga
     1681 atttcgcgtg gcctaccgtc gcaaaaacaa cagctttggc attccaagcg aatttgccaa
     1741 tttcccgaac tttgcgttcg atgatttggg ttacaccttt ggtccacagg gtgaatcccc
     1801 acagggtgaa atcaccaaca gctatcagta tgttgataac ttgacctgga ttaagggcaa
     1861 gcacaacctc aagtttggtg tggaattccg gaatgtgatt tctgctaacg gcttcctgcc
     1921 acgtggccgt ggtgagtatg actatgccac gttggaacag ttcctgcgcg atgtaaaacc
     1981 aaccggcttt aacggtgggt tgaaaggtgt tggaacatca gcctttgcgg ccaaccgcaa
     2041 gtcaatctac tggtatgtcc aggacgatat caaagtgact ccgacgttta ctctcaacgt
     2101 tgggctccga tatgaattct attcactctt ccgcgatgaa aaactccagg cgttgaatgc
     2161 aattgcaagc gttcctggtg tgattgaatt tggtgtacca aagtcagaca
//
LOCUS       DAOQDO010001061         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46336_length_2210_cov_3.077030, whole genome shotgun sequence.
ACCESSION   DAOQDO010001061 DAOQDO010000000
VERSION     DAOQDO010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46336_length_2210_cov_3.077030"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKZ53_15835"
     CDS             complement(<1..193)
                     /locus_tag="PKZ53_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41959.1"
                     /translation="MAALILLLMNDDSVQAASQSSLQAGIIPFETVTLAPEIPSLSLE
                     AALSQGLQVSGVEVLPSNLV"
     gene            629..703
                     /locus_tag="PKZ53_15840"
     tRNA            629..703
                     /locus_tag="PKZ53_15840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Gly,seq:ccc)
     gene            complement(956..2071)
                     /gene="dnaN"
                     /locus_tag="PKZ53_15845"
     CDS             complement(956..2071)
                     /gene="dnaN"
                     /locus_tag="PKZ53_15845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNJ41960.1"
                     /translation="MEFSVNRSDLLKELNFLQGVVEKKSTIPIIQNLLIRAKKGEPLT
                     LVATDLESTLRCLCPAQVKVGGTILLPARKLYDLVRSLPDAEITVKTDGNDQAIVTCE
                     RSRFKLLSPDPEAFPPLANVPDSQFSVPASILRAMIPRTLFASTQEESRFALNGAQVI
                     LSPSTLRVVATDGHRLAFIEKSGVEFKAPKESIKLLIPKKTLAEVQKLAGDADDEIIK
                     FGKDDNHIFFEIGNRLYISRMLAGQFPNYEMVMPKANDKALIIESDRLAATLRRVAYM
                     ADDVTHAIKINITEGQIEFSAQSSGSGEGFDALPVPYDGDTYSLAFNVQYLLDFLSVV
                     NVGEISFEFRDEATQIQLRPHGESDYNYRYVVMPMRN"
BASE COUNT          589 a          500 c          537 g          584 t
ORIGIN      
        1 caaccagatt cgacggcagc acctcaacac cactgacctg taacccctgg ctcagggcgg
       61 cttcgagtga gagtgatggg atctcaggtg ctaacgtcac agtttcaaac gggataatcc
      121 cggcttgaag tgatgattgc gatgccgcct gcaccgagtc atcattcatc aacagcagga
      181 tcagtgcagc caaaagcagg cgccacgcat ggtgtctgat gaaatacatt ggtttgatca
      241 gccggaaggt tgttgctgat aatgccaatt tagaatgaag aatgaagaat aaagaatgat
      301 tcaaacgcat tgattccaga tatttaatgg tcccataaaa tataaggagt ccatttcaaa
      361 tagtataaga gtgaaatttg gtgggacgta acggagatgt atcaggagaa tttccacttt
      421 tcatttttcg attgaacacc tctaccagtt tgaaatgaac tcatcggatt aggaagttat
      481 taaatacctg gaatcaattg gcttaaccaa ttcttcattc ttcattcttc attctttatt
      541 ctaaattggt attagctgtt gacagaagcg cctcggagga attatttttg gcgtccttaa
      601 agaccatctg acaatctgga tgtgctgagc gggcgtagct caatggtaga gtaccagctt
      661 cccaagctgg gtgttgtggg ttcgagtccc atcgcccgct ccattttatt tccccttaca
      721 aacattcccc tttaacgatt tagctcagca aattggtgct tggtgcttgg tgcttggtgc
      781 tttggaatca attcagtcca aagaatcaag aactatctag tggttagtgg ttagaaatca
      841 atattttcga agaaaaacca agaatcaggt atcaaaaacc aagcaccaaa aaccaagcac
      901 caaaaaccaa gcaccaaaaa ctaagaacca agaaccaggc accagtcacc aaaccctaat
      961 tcctcatcgg catgaccaca tagcgatagt tgtagtcact ctcgccgtgt ggccgtaact
     1021 ggatttgcgt ggcttcatca cggaattcga aactgatttc cccaacattg acgactgaga
     1081 gaaaatcaag caggtactga acgttaaagg ccagtgaata agtgtctccg tcatagggaa
     1141 ctggtaatgc gtcaaagcct tcgccggatc cacttgactg ggcgctgaac tcgatctggc
     1201 cttcagtaat gttgatttta atggcgtggg tgacatcgtc agccatatag gcgacgcggc
     1261 gtaacgtcgc ggccagacga tctgactcaa tgatcagtgc tttatcgttt gccttgggca
     1321 tcaccatttc ataattgggg aactgaccgg cgagcatccg tgagatgtag aggcggttac
     1381 caatctcaaa aaagatgtgg ttgtcatctt tgccaaactt gatgatttca tcgtcggcat
     1441 ccccagccag tttttgaacc tcagccagtg tctttttcgg gatcagcagt ttgatgcttt
     1501 ctttgggggc tttgaattca acgcctgatt tttcaataaa ggccagtcgg tggccatctg
     1561 tcgccacaac ccgcaaggtc gaggggctga gaatcacctg ggcaccgttg agggcaaagc
     1621 gggattcttc ctgagtggag gcaaacaacg tccggggaat catggcgcgt aagatcgatg
     1681 ccgggactga aaattgtgaa tcagggacat ttgccagtgg cgggaaggct tctggatcag
     1741 ggcttaacag tttaaatctg gagcgttcac acgtcacaat tgcctgatcg ttgccatcgg
     1801 ttttgacagt gatttcagcg tctggaaggc tacggaccag gtcatacaat ttccgggctg
     1861 gaagcagaat ggttcctccg acttttacct gagccgggca gagacaacgc agcgttgatt
     1921 ccagatcggt ggccaccagg gtcagcggtt cgcctttttt ggcgcgaatc aacaggtttt
     1981 gaatgatagg aatggtgctt ttcttttcca ccacgccttg cagaaagttt aactctttca
     2041 gaaggtcaga acgattaact gaaaattcca cggctggatt ccttttcttg ttagcgaaga
     2101 tacagtgtgt ggtatagtgg gccgccatta tgattgagcc actggccaga tgcaatccta
     2161 acccaattcc cagggggtgg gtgttttaac ctgcaatttc caatcttctc
//
LOCUS       DAOQDO010001062         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46368_length_2209_cov_3.681523, whole genome shotgun sequence.
ACCESSION   DAOQDO010001062 DAOQDO010000000
VERSION     DAOQDO010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46368_length_2209_cov_3.681523"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1018)
                     /locus_tag="PKZ53_15850"
     CDS             complement(152..1018)
                     /locus_tag="PKZ53_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41961.1"
                     /translation="MKPFILKGLLITGLFFFVGAAQAPVHRIDWSKMKAAGTQLPGVL
                     ASGEIPDKLDALRIENTTSQPVTIKLATLEAPRLSQPQWRLTGRIRYQNVEGEGYLEL
                     WNHFNQGSYFTRSLADSGPMGKVKGSSEWRDFTLPFDASTGNAQLKLMKLELNLILPG
                     KGVVFIGPVEVHEGFGSAGWWNDPSANWLGAILGVSLGCLGALVGILSSLGKARQLVL
                     WLCQGGFVVGILLLAAGLVALGMGQSYAIYYPLLLCGLISGLVFGLNYRTLFRRYQEL
                     ELRQMQARDAML"
     gene            complement(1015..1341)
                     /locus_tag="PKZ53_15855"
     CDS             complement(1015..1341)
                     /locus_tag="PKZ53_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNJ41962.1"
                     /translation="MADMVSPPSQTWLKFLSDEDLAFIRNFILASGSLKELALVYGVS
                     YPTLRLRLDRLIEKIKIAEAHQVTSHFEGTLRALYAEGKIDLETLRALLAAHKLEMEN
                     QGGNQS"
     gene            1770..>2209
                     /locus_tag="PKZ53_15860"
     CDS             1770..>2209
                     /locus_tag="PKZ53_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ41963.1"
                     /translation="MEKILRRNNVRVTGRGQQSLFLAHGFGCDQNMWRYVVPAFQDEY
                     RIVLFDYVGAGQSDLTAYDPTRYSSLDGYAQDVLDICLALELKDVVFVGHSVSSMVGA
                     LASIRSPEFFSHLIMVGPSPCYLNHPPDYFGGFEREDILGLLDM"
BASE COUNT          542 a          546 c          542 g          579 t
ORIGIN      
        1 ttgaaagata gcgacttgtc gctaaactac ttcagtgcga ccaaaggttg gtagcaacaa
       61 gtcgctacgt gggaaagtgg tgacacgtca ccgcactcca aatattgaag gatcgacaat
      121 ctggtaagaa tcgcaaccaa actgtgagct gtcacaacat cgcatctcgg gcctgcatct
      181 gccggagttc gagttcctga tagcgtcgaa agagggtccg gtaattcaag ccaaacacca
      241 atccgctgat caggccgcac aacagcaatg gatagtaaat ggcatacgat tgacccatcc
      301 caagcgctac cagcccggcg gccaggagca aaatccccac cacaaaccct ccctggcaca
      361 accacaacac aagctggcgt gcttttccaa ggctggaaag aattcccacc agggctccta
      421 aacagccaag gctgacaccc aggatggcgc ccagccagtt ggcgctgggg tcattccacc
      481 acccagcaga gccaaaccct tcgtgcacct caaccggtcc aatgaagacc acgccttttc
      541 cgggaaggat caggttcaat tccagtttca tcagtttcaa ttgggcattt cccgttgacg
      601 catcaaacgg caacgtaaaa tcgcgccatt cggatgatcc tttgaccttt cccatcgggc
      661 cggaatcagc cagggaacga gtaaagtatg acccctgatt gaaatgattc cacaattcca
      721 gatacccctc gccttcaaca ttttgatagc ggatgcgtcc ggtcagccgc cattgtggct
      781 ggctcagccg gggagcttcc agcgtggcaa gtttgatggt gacaggctgg ctggtggtat
      841 tttcgattcg gagggcatcc agcttgtctg ggatttcacc tgatgccagt accccaggga
      901 gttgcgtgcc agccgctttc atttttgacc agtcaatgcg gtgaactggt gcctgagccg
      961 cacccacgaa gaaaaacaag ccagtaatca gtaacccttt gagaataaat ggtttcatga
     1021 ttggtttcct ccttgattct ccatttccag tttatgggct gccagcaagg cgcgcagggt
     1081 ttccaggtcg attttgcctt cggcatacag ggcgcgcagt gtgccttcaa agtgactggt
     1141 tacctggtgc gcttcggcaa ttttgatttt ttcgatcaac cgatccagcc gaagccgcaa
     1201 tgtggggtag gacacgccat agaccaacgc cagttccttg agtgaccctg aagccaggat
     1261 gaaattgcgg ataaatgcca gatcctcgtc cgaaagaaat ttcaaccagg tttgagaagg
     1321 cggagacacc atgtcggcca tgtggaataa gcactttctt taataatatt aaagtttatt
     1381 tgaattatat tcttaaattt tattaagtca atttaaaatt tattaaaatt ttgatcaaat
     1441 tgaatcaatc tgagctggcg tatgacggtc atctcagtgt gagatcagaa gacgttcggg
     1501 tgtgtttttt ctggtttctg gttctggata tttatggctg taagccggaa taaaggtgag
     1561 gctgggtgct tgccaacctg tgagtgctat gccaagctta gaaacccaag tcatcatgcc
     1621 cagaaatctc ttttattccc tgacacatcc gccaaacatc aggcgttatc gaggaacctc
     1681 tcttgagttg gccagtcttt ggtgcacctg cgtgatttgg gtttcgtgtc gaaattcaat
     1741 ccacagtgcc gcagattcac caaaatctca tggaaaaaat tcttcgtcga aacaacgtcc
     1801 gggtgactgg acgtggtcag caatctttgt tcctggccca tggctttgga tgcgaccaga
     1861 acatgtggcg atatgtggta cctgcctttc aagatgagta ccggattgtc ctttttgact
     1921 atgtaggggc tggtcaatca gacctgacgg catatgatcc aactcgctat agttctttag
     1981 atggctatgc ccaggacgtg cttgatattt gtctggcgct cgaactgaag gatgtcgttt
     2041 ttgtcggtca ttcggtcagt tccatggtgg gggcgcttgc ctcgatcagg tcacccgagt
     2101 ttttcagcca tttgattatg gttggccctt caccctgtta tttgaatcat ccaccagact
     2161 attttggtgg ttttgaacgt gaagatattc tgggtttact cgatatgat
//
LOCUS       DAOQDO010001063         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46372_length_2209_cov_3.107707, whole genome shotgun sequence.
ACCESSION   DAOQDO010001063 DAOQDO010000000
VERSION     DAOQDO010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46372_length_2209_cov_3.107707"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /gene="tssB"
                     /locus_tag="PKZ53_15865"
     CDS             <1..516
                     /gene="tssB"
                     /locus_tag="PKZ53_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNJ41964.1"
                     /translation="RVRPPRVQITYDVEIGDAIEMKELPFVVGVMGDFSGKSLAEMPR
                     VKDRKFVEIDRDNFNQVMAKMKPKIAMRVENKLADTNTQLAVELEFKTMDDFHPDALV
                     QQVPALRKLIEARRKLSDLLSKMDGNMKLEELLTDVVQNTEKQRQLGRVLGLAAPIEA
                     TLVEEEGNSNE"
     gene            509..2005
                     /gene="tssC"
                     /locus_tag="PKZ53_15870"
     CDS             509..2005
                     /gene="tssC"
                     /locus_tag="PKZ53_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNJ41965.1"
                     /translation="MNSQLQSFAPAAASAVDFSDQSLLDQLITEGRMAQDETQEAMAK
                     DLVTEFVQQVLDGELVISKDLESAIMQRIAEIDSLLTGQVNEILHHQEFQKMEASWRG
                     LQYLVFETETSTQLKIRVLNVSKKELLKDVTNAIEFDQSAIFKKVYEEEYGTFGGAPY
                     GCLIGDFEFTNHPQDMALLEKMSGVAAAAHAPFISAASPKLFGWESFTQLGEVRDLAK
                     IFDRIEYVKWRSFRDSEDSRYVGLCLPHNLLRLPYGTETAETETFRFEEDVTGRDHKK
                     YLWGNAAYALGVRVTEAFAKHGWTVAIRGVEGGGLVQGMPTHTFMTDDGEVAMKCPTE
                     IAITDRREKEFSDLGFIPLVHCKNTDYAAFFGTNSANKPKKYDTDFANANARLSSQLQ
                     YIFAVSRFAHYLKAIMRDKIGSFMERKNVEDYLNRWITNYVLIDDNATQEQKSKLPLR
                     EARVEVAEVKGKPGCYKAVSYLRPHFQLDEIAISLRLVSELPAPANQR"
BASE COUNT          602 a          503 c          575 g          529 t
ORIGIN      
        1 cgtgtcaggc cgccacgggt tcaaattacc tatgatgtgg aaatcggcga cgctatcgaa
       61 atgaaagaac tcccgtttgt ggtcggcgtg atgggcgatt tttctggtaa atccctggcg
      121 gaaatgccgc gcgtgaaaga tcgaaaattt gttgaaattg accgtgataa cttcaaccag
      181 gtgatggcca aaatgaagcc gaaaattgcc atgcgggttg agaacaaact ggctgatacc
      241 aatacccaac tggcggtcga acttgaattt aaaacgatgg atgattttca tccggatgcg
      301 ctggttcaac aggtgccagc gctgcggaaa ctgatcgaag cccggcggaa attgtcagat
      361 ctgctgtcaa agatggacgg caatatgaag cttgaagaat tgctgaccga cgtggttcaa
      421 aacacggaaa aacagcgcca gcttggacgc gttttgggat tggccgcccc gattgaagca
      481 accttggttg aggaggaggg caattccaat gaatagccag cttcaatcat ttgcgccggc
      541 tgcggccagt gccgtggatt tctcagatca gtcgctcctg gaccagttaa ttaccgaagg
      601 ccggatggcc caggatgaaa cccaggaagc gatggccaaa gatctggtca ctgaatttgt
      661 ccagcaagtc ctcgacggcg aactggtgat ctccaaagat ctggaatcag ccattatgca
      721 gcgaattgca gagattgact cgctgttgac cgggcaggtc aatgaaatct tgcaccatca
      781 ggaatttcag aaaatggagg cttcctggcg gggactgcaa tatctggtat ttgaaaccga
      841 aacaagcacg cagttgaaaa ttcgagtcct taacgtttca aaaaaagagt tgctcaagga
      901 tgttaccaat gccattgagt ttgaccagtc agcaattttc aaaaaggtct atgaagaaga
      961 atatggcacg tttggcggcg ctccgtatgg ttgtttgatt ggggattttg aatttaccaa
     1021 ccatccccag gatatggcat tgctggaaaa aatgtcaggt gtggcggctg cggcccacgc
     1081 cccatttatt tcagcggctt caccaaagct ctttggctgg gaaagtttta cccaacttgg
     1141 tgaagttcgc gacctggcca agatcttcga ccggattgaa tatgttaaat ggcgttcctt
     1201 ccgggactcg gaagattcgc ggtatgttgg gctgtgtctg ccacataacc tgttgcggtt
     1261 gccctatggt acagaaacgg ctgagacgga aaccttccgg tttgaagaag atgtgactgg
     1321 ccgcgatcac aaaaagtact tgtggggaaa tgccgcctat gccctgggtg tacgcgtgac
     1381 agaagctttt gccaaacacg gttggaccgt ggccattcgc ggtgttgaag gcggtggtct
     1441 ggtgcaggga atgccaaccc atacttttat gactgacgat ggcgaagtgg cgatgaagtg
     1501 cccaaccgaa attgccatca ctgaccgccg tgaaaaagag ttcagtgacc tgggctttat
     1561 ccctctggtg cactgcaaga acaccgatta tgcggcgttt tttggcacga actcagccaa
     1621 caagcccaaa aaatacgaca ctgactttgc caatgccaac gcccggctgt cgtcccaatt
     1681 gcagtatatc tttgcagtga gccggtttgc gcattacctg aaagcaatta tgcgtgacaa
     1741 gattggctcg tttatggagc gcaagaacgt tgaggattat ctcaaccgct ggatcacaaa
     1801 ttatgtgtta attgatgata acgccactca ggagcagaaa tccaagctac cactgcgtga
     1861 agcgcgagtc gaagtcgccg aagtcaaagg caagcctgga tgctacaaag cggtcagcta
     1921 tctgcggcca catttccagc ttgatgaaat tgccatttct ttacggctgg tttcggagct
     1981 tccggcccca gccaatcaac gataaaaata ccaatttgga atgaagaata aagaatgaat
     2041 taaatctact gttttcaggt atttaatggt ttctttatac gaaaagctca ttccaaaatg
     2101 gtacaaccag tgcgtagtat ctggtgctca ggagctggta cttgcgaaaa gaactaagca
     2161 ccaggaatcc acccaaactt actcacttaa ccacacttaa aggagatct
//
LOCUS       DAOQDO010001064         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46388_length_2209_cov_2.371866, whole genome shotgun sequence.
ACCESSION   DAOQDO010001064 DAOQDO010000000
VERSION     DAOQDO010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46388_length_2209_cov_2.371866"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKZ53_15875"
     CDS             complement(<1..698)
                     /locus_tag="PKZ53_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41966.1"
                     /translation="MALAKLGTALSVQAAAGLIDASAASEAEAGASPPAEPNSDFAAN
                     GSVLAVASGGLLSAIDLRHSYRVLRLASHPATIRQVKVSANGRFVATFADDQSLRLFS
                     LPTGVLVLDLPLSLDFLALGERRHAELFAFSPDSKRLLALDCGERSGPACPLLRMRVF
                     DSERGERRSEIALPAEPVDYTSRSDGTVAVLPQGGLPRFFDADSGMEWPQPASAAAVA
                     GKPDTARKQCLTAR"
     gene            1400..>2209
                     /locus_tag="PKZ53_15880"
     CDS             1400..>2209
                     /locus_tag="PKZ53_15880"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ41967.1"
                     /translation="MLVCPKCNAVYNDKLKYCSVDGNLLVPGFPETYLAVGQVLNESL
                     RVVARWRADRLGEVYQAEETFMSGSYVALRLFRPGLITSDIRKALEGLADSIRGMLPD
                     PDVITGYFTVEFTDNRYALVSGYVHGRLLDDIVIQEAPLPPARVVALLLRLTEICANL
                     HQDGWIHGDLTPESIIITGFDETEGYSLKILDYGLARIIAQFGKRSLCGPTGHLAVRN
                     YETYLAPEILGPDPSLITHRADIYSLGALCYHLLSGHLPFFFDQFGITGPVY"
BASE COUNT          455 a          622 c          664 g          468 t
ORIGIN      
        1 aagcgagccg tcagacactg cttgcgggca gtgtcgggct taccggcgac ggcggctgcg
       61 gacgcgggct ggggccactc catgccgctg tcggcgtcga agaagcgcgg cagcccgccc
      121 tgcggcaaga ctgcaacggt gccatccgag cggctggtgt agtcgacagg ctcggccggc
      181 agggcgatct cgctgcggcg ctcaccgcgc tcgctgtcaa agacgcgcat gcgcagcagt
      241 ggacaggccg gcccgctgcg ctcgccacag tcgagggcca gcaggcgctt gctgtcgggg
      301 ctgaatgcga atagctcggc atggcggcgc tcgccgaggg ccagaaagtc gaggctgagc
      361 ggcaggtcca ggaccagcac gccggtcggc agcgagaaca ggcgcaggct ctggtcgtcg
      421 gcgaaggtgg cgacgaagcg gccgttggcg ctgaccttga cctggcggat ggtcgccggg
      481 tggctggcca gccgcagcac gcggtagcta tggcgcagat cgatggcgga cagcaggccg
      541 ccgctggcca cggcgaggac gctgccgttg gcggcaaagt cgctgtttgg ctcggccggt
      601 gggctggccc cagcctcggc ttcggatgca gcgctggcat cgattagacc ggcggcagcc
      661 tgcacgctga gcgccgttcc cagcttggcc aaggccatgc cgatcaggga actcgatcag
      721 gccctgtcgc gcaacgcgat cgagcgccgt caccgcccgc cggtcaccaa ttttgtgaag
      781 tgcctcaact gattgcaacc gcacgcgcgg gtcgggatca tccaaaactt tgatgagcgc
      841 atcaaccgcc attgcatcgc caatcaatcc aagtttggcg gcggcttctt cacggatttc
      901 tgggtcagtg gacgtcaggt tttcaatcaa tgttgacaca taagactccg aatggtcggc
      961 agaacggctt acaggttggc tcattgtatc tgatggaaat ggatggagat tcggaaagtg
     1021 gtctcatcat atgcaattgg cattttgaat tcaaccatct gtctgatgca gtacgaggat
     1081 gtctgtcaga tttcggcgaa acacgtcagc aagccaaaca aggtgactca cacattgacg
     1141 gacggatagc cgtgaaacta cactcgggga gtttgaagca cggctgggct ggcatctccc
     1201 cataccttgc ctgacaagct ctttgggctg aagaaaacag ggctggaacg aaagaaaata
     1261 ggttaaactg gaatttgacg actcggtccg gcgtttcttc cccagactgc cggccttctt
     1321 tcacccactg cccacacttc ttcaacccga aagaaaccgg cggcgtcatt cgtcgcacac
     1381 cataagaaga ctcacaccta tgctggtttg tccaaagtgc aacgcggttt acaacgacaa
     1441 actcaagtac tgcagcgtgg atggaaatct cctggtacct ggatttcctg aaacctacct
     1501 ggcagttggg caagtgctga acgaaagctt gcgggtggtg gcacgctggc gggctgatcg
     1561 ccttggcgaa gtctatcagg ctgaagaaac ctttatgagt ggcagctatg tcgccctcag
     1621 attgtttcgc cccggtttga ttaccagcga tatccgcaag gcacttgaag gactggccga
     1681 ttcgattcgt ggcatgttgc cggatccaga cgtcatcaca ggctatttta cggttgaatt
     1741 tactgataac cgctatgccc tggtttccgg atacgttcat ggtcgcttgc ttgacgatat
     1801 tgtgattcag gaagctcccc tccccccagc ccgggtggtg gcgctcttgc tccggctgac
     1861 tgaaatttgt gccaacctgc accaggatgg atggattcac ggtgatctaa ccccggaaag
     1921 catcatcatc accgggtttg atgagacaga aggatacagc ctgaagattc ttgactacgg
     1981 gctggcacga atcattgccc agtttgggaa acgttcgctg tgtggtccca ctggccatct
     2041 ggcggtccgc aactatgaaa cgtacctggc acccgaaatt ctagggccag atccgtcact
     2101 gattacccac cgggccgata tttattcact tggtgccttg tgttatcacc tgttaagcgg
     2161 ccatttgccg tttttctttg atcaatttgg gattaccggg ccggtctat
//
LOCUS       DAOQDO010001065         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46416_length_2208_cov_3.200650, whole genome shotgun sequence.
ACCESSION   DAOQDO010001065 DAOQDO010000000
VERSION     DAOQDO010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46416_length_2208_cov_3.200650"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="PKZ53_15885"
     CDS             <1..1792
                     /locus_tag="PKZ53_15885"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNJ41968.1"
                     /translation="EELALTTGKILVVTGGFHTVVLPQLIQQPVKRPKKPKLAEGDAQ
                     TVVMRYSFEQLDALNGYAAGMPSPNYYQQTWEHLQAHPTPSPSAPSPQPSAPAIAVAA
                     HFLITIGRLTRDQNGMTPLSSVDEISALGQARQLARFRGHTSGPTREDLLDGIRSCFV
                     KGAMDTEGKLLLETVQKLLTGDQIGDLPPTVGVPPLVEDFRCEATSLRLKIGDSTPKS
                     INLDVYRKKTHRRASRFFHRLKFLQVFFARLTAGPDFARGTGLERLQEQWEYQWTPLV
                     ETKLVEQSLFGTTVEEAALNLLVKTISDLETEGKSRSAIQAVRLLVAACQMGLHRSVS
                     RLLALISENIHQDPDFSSLVEALEQLLLLWQSREPLEAHHLTVLPEFSQAAFHKACFL
                     IPELAGCPAEMATNVVESLASLREMISSTPDQILDETLFYSALEPIPLKPDGNPLVCG
                     GVVGILFSAGKISESAVLHFVRGFLEGASASTSDQIGFLQGLLKTCREAAWNLSGLLA
                     LLDEYLTAWSEDDFLRILPELRLSFADLTPRETDRVAEMVAHLHGQPQLNLPDTRTLS
                     EEALRFGLHLNQLILESLHHDGLAEKFGVR"
     gene            complement(1840..>2208)
                     /locus_tag="PKZ53_15890"
     CDS             complement(1840..>2208)
                     /locus_tag="PKZ53_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41969.1"
                     /translation="EYLQTAATASTVKAVLPGPTYIWYRAQWLQRQEAERRFARLLAM
                     IRMVGYLVFLCGMGGWLLVHWPDIQREVGQVSPWLVTRVQSGLALVSQPLGYLTITLF
                     GINFGLTFWSLWRERQTHTA"
BASE COUNT          527 a          630 c          574 g          477 t
ORIGIN      
        1 ggaagaactg gccctcacga ccggaaaaat tctggttgtt acaggcgggt ttcacaccgt
       61 ggtgctcccc caactgatac agcagccggt caaacgtccc aaaaagccaa aactggcaga
      121 aggtgacgcc caaaccgtgg taatgcggta cagctttgaa caactcgacg cgctcaacgg
      181 ctatgccgcc ggaatgcctt cgcccaacta ttaccagcag acgtgggagc acctccaggc
      241 tcacccaacc cccagcccct cagcccccag ccctcagccc tcagccccag ctatcgcagt
      301 ggctgcccac tttctgatta caatcggacg actgactcgc gaccaaaatg gaatgacacc
      361 gctctcgtcg gttgatgaaa tctcggcgct gggccaggcc cggcaactgg cccgatttcg
      421 aggacacacc agcggcccaa cccgcgaaga cctcctcgac ggcatccgga gttgctttgt
      481 gaaaggcgcg atggacacgg aaggaaaact cctgcttgag accgtgcaga aacttttgac
      541 tggagatcaa attggtgatc tgccgccaac ggttggggtt ccgccactgg ttgaagactt
      601 tcgctgtgag gccaccagct tacggcttaa aattggagat tccaccccca aatcaatcaa
      661 cctggatgtg taccgaaaaa aaacacatcg ccgggcaagt cggtttttcc atcgcctgaa
      721 gtttctccag gtgttttttg ctcgattgac ggcgggaccc gattttgcac gcggaacagg
      781 tctggaacgg cttcaggaac agtgggagta ccagtggaca ccgctggtgg aaacgaaact
      841 ggtcgaacag tcgctctttg gcaccacggt tgaggaagcc gcgctcaacc tgctggtcaa
      901 aaccatcagt gatcttgaga cggaaggcaa atcccgaagc gccatccagg ccgtccgatt
      961 gctggtggcg gcctgtcaaa tgggtctgca ccggtcggtt tcgcggttgc tggcgctgat
     1021 ttccgaaaat attcaccagg atccggattt ttcttcgctg gttgaagcgt tggaacaatt
     1081 actcctgctc tggcaatcac gcgaaccact cgaagcacac cacctgacgg tattgcccga
     1141 gttcagtcag gcagcctttc acaaagcctg ttttctgatc ccagaacttg ccggttgtcc
     1201 ggcggaaatg gcgacgaatg tcgtggaaag cctcgcttcc ctgcgcgaaa tgatcagttc
     1261 caccccagac cagattttag atgaaactct gttttattca gcgctggaac cgattccctt
     1321 gaaaccggat gggaacccgc tcgtgtgtgg aggcgtggtt ggaattctct tcagcgctgg
     1381 aaaaataagc gagtcagccg tcttacactt tgtcagagga tttctggaag gggcttcagc
     1441 ctcaaccagc gatcagatcg gctttctgca gggacttctg aaaacctgtc gtgaagcggc
     1501 ctggaatctg tcaggattgc tcgccttgct ggatgaatat ctgaccgcct ggagcgaaga
     1561 cgatttttta agaatcctgc ccgaactccg gctgtcgttt gccgatttga cgcctcgtga
     1621 aactgaccgg gtggctgaaa tggtggctca tttgcacggc caaccccaac ttaacctgcc
     1681 cgatacccga accctctctg aagaagccct ccggtttggc ttgcacctca atcaattgat
     1741 tctggaaagc ctgcaccatg atggattagc agaaaaattt ggagtgcggt gacctgtcac
     1801 cgcttttgta ttgagcaatg tggcacgaac tcatcctggt caggccgtgt gagtctggcg
     1861 ttcacgccac aaactccaga acgtaagccc aaagttgata ccaaacaagg tgatcgttag
     1921 ataaccaagc ggttgtgaga cgagagccag accagactga acccgagtca ccagccaggg
     1981 tgaaacctgc ccgacttccc tttgtatgtc tggccagtga accagaagcc atcctcccat
     2041 cccacacaga aaaaccagat acccaaccat tcggatcatt gccagcaacc gggcgaatcg
     2101 tcgttcagct tcctgtcgtt gcagccattg agcccggtac cagatatagg taggaccagg
     2161 taaaacagcc ttcacagtcg aagcggtggc ggctgtttgc agatattc
//
LOCUS       DAOQDO010001066         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46591_length_2204_cov_2.813867, whole genome shotgun sequence.
ACCESSION   DAOQDO010001066 DAOQDO010000000
VERSION     DAOQDO010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46591_length_2204_cov_2.813867"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2204)
                     /locus_tag="PKZ53_15895"
     CDS             complement(<1..>2204)
                     /locus_tag="PKZ53_15895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ41970.1"
                     /translation="NRIVGVQGIARDISRRRQTEEQLRKSDERFQLVARATNDAVWDW
                     DITSDVLWWNDGLYLLFGYQLDQIQPNISWWFDQIHSDDRERVRTGILNCLSQREKNW
                     SDEYRFYRADSSIAYVFDRGFVVFDQQGHPSRMIGAMLDITARKQAEEALHESEALYR
                     TLFENAPIGLGIADVDGNLIGFNRAILEPGGYTEADIHQIQNVGDLYLDENQRDTIRD
                     IFEKYGFVRQHEVRFRRKAGGFYDALLSLVPVRIKGQPCIQAMVEDITERKLASEALK
                     QSEHRYRTISEVTSDYAYAVRIEPDGTMIREWISGAFSQITGYTVEELDETVNWRAVT
                     HPDDLEIGRAHLVTIRKGQPNTCELRIVTKQGTERWLRVQGQPVWDKEQNRVVQFYGA
                     AQDITERRLAELALKESEERFRDLFENATDLIYTHDLQGNFTSINGATEHITGYTREE
                     GVQMNIRQIVAPESYDLARQMIAHKVNGGPSTTYEIDIIGKDGRRVPLEVSTRLLTEH
                     GQPIGVQGIARNITERKQAEKALRESEEQYKLLFASNPHPTWIYDLETLAFVVVNDVA
                     IEHYGYSREEFLAMTVLDLRVPEEIPLFQEHLNNTARQKIERSGIWRHRKKDGRLIEV
                     ETTSHELVFQGRPCRIVLANDVTERNQMESALRESEAKNRALLDAIPDSIYRIGHDGT
                     LLDMKLRRNFEEPLQSIMDIGDNIFHILPPQVSEGARQAIELVFATREIQIY"
BASE COUNT          492 a          579 c          565 g          568 t
ORIGIN      
        1 atagatctgg atctcgcgcg tggcaaaaac aagttcaatg gcctggcggg caccttcact
       61 gacctgaggc ggcaaaatgt gaaagatgtt gtctccaata tccataatgg attggagtgg
      121 ttcttcaaaa ttacgccgaa gcttcatatc aagcagcgtg ccgtcgtgcc cgatgcgata
      181 gatcgagtca ggaattgcat cgagcagcgc ccggtttttg gcttcgcttt cccggagtgc
      241 cgattccatt tgattgcgtt cggtcacgtc attggccagc acaatgcggc agggacgccc
      301 ctgaaatacc agttcgtgcg aggtcgtttc aacttcgatc agccgcccat cttttttccg
      361 gtgtcgccag attccagacc gctcaatttt ttggcgagca gtgttgttga gatgttcctg
      421 gaacaacgga atctcttccg gaacacgcag atccagcacg gtcatcgcca ggaactcttc
      481 gcggctgtag ccataatgtt cgatagcgac atcgttgacc acaacaaagg ccagagtttc
      541 aagatcatag atccaggttg ggtgcggatt gctggcaaac agaagcttgt attgttcttc
      601 ggactcacgc agggcttttt cggcctgctt gcgttccgta atattgcggg caattccctg
      661 aaccccaatc ggctggccat gttcggtgag taaccgggta ctgacttcaa gtggaacgcg
      721 tcgtccatct ttcccgatga tgtcaatttc gtaggtggtt gatgggccgc cgtttacttt
      781 atgggcaatc atttgccggg ccagatcata agactcgggg gccacaatct ggcgaatgtt
      841 catctggacc ccttcttcgc gggtatagcc agtgatgtgt tcagtggcgc cattgatcga
      901 ggtaaaattc ccctgcaggt cgtgggtgta aatcaggtca gtcgcatttt caaacagatc
      961 ccggaaccgc tcttcacttt ctttgagcgc cagttccgcc agccggcgct cggtaatatc
     1021 ctgagccgcc ccataaaact gaacgacccg attttgctcc ttatcccaga ctggctggcc
     1081 ctggactctc agccagcgtt ctgtgccctg tttggtcaca attcgaagtt cacaggtgtt
     1141 gggttgacct ttgcggatgg taaccaggtg ggcgcgtcca atttcaagat catcagggtg
     1201 tgttactgcc cgccagttga ccgtttcatc cagttcctca acggtatagc cggtaatttg
     1261 tgagaaggca ccggagatcc attcacgaat catggtgcca tccggttcga tccgaacggc
     1321 ataggcgtaa tcagaagtca cttctgaaat cgtgcgatag cggtgctcgc tttgttttaa
     1381 ggcttcactg gccagtttgc gttcggtgat gtcttccacc atcgcctgaa tacagggttg
     1441 tcccttgatt cgaaccggta ccaggctgag cagggcatcg taaaagccac ccgccttgcg
     1501 tctgaatcgg acttcgtgtt gccggacaaa tccatatttt tcaaaaatat cacgaatggt
     1561 atcgcgctgg ttttcatcca ggtaaagatc accgacattc tgaatctggt ggatatcagc
     1621 ttcggtatag cctccaggtt ctaaaattgc ccggttaaac ccaatcaaat tgccatccac
     1681 atcagcaatt ccaagcccaa tcggggcatt ttcaaacaag gtccgataga gggcctcgct
     1741 ttcgtgcagg gcctcttcgg cctgtttgcg ggctgtaata tcgagcatgg caccgatcat
     1801 gcgcgatggg tggccttgct ggtcaaaaac cacaaacccc cggtcaaaaa cataggcaat
     1861 tgaactatcc gcacggtaaa agcggtattc gtccgaccag ttcttctcgc gttgactgag
     1921 gcaattcagg attccagtgc gtacccgttc ccggtcgtcg gaatggatct ggtcaaacca
     1981 ccaggaaata ttgggttgta tttgatcaag ttgatagcca aacagaaggt aaagcccgtc
     2041 gttccaccag agcacatcac tggtgatatc ccaatcccaa acagcatcat tggtggcccg
     2101 ggccaccagt tgaaagcgtt catcgctttt gcggagttgt tcttccgttt ggcgtcgcct
     2161 ggaaatatcg cgggcaattc cttgaacgcc aacaatgcgg ttgc
//
LOCUS       DAOQDO010001067         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46601_length_2204_cov_2.545835, whole genome shotgun sequence.
ACCESSION   DAOQDO010001067 DAOQDO010000000
VERSION     DAOQDO010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46601_length_2204_cov_2.545835"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..532
                     /locus_tag="PKZ53_15900"
     CDS             119..532
                     /locus_tag="PKZ53_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41971.1"
                     /translation="MLLKTELFQCLMVISQESPNFDKSFAWAEKCLKLLGESDDIADR
                     LLDLVPPEFPWQSLADVLGMWMWMTSDNGAAILHTTERWLEEATDERKCLIALNLDAY
                     PFQNADQMRDVLLRVSLLSFELREQCLVLMASRQN"
     gene            561..1376
                     /locus_tag="PKZ53_15905"
     CDS             561..1376
                     /locus_tag="PKZ53_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ41972.1"
                     /translation="MSQDTPQDHISRKLVLFQLPDQDRVTIRQDIVFQTVGDDVLTLD
                     LYVPADAKSGDLLPAVIIVTGYPDVGFQKLVGCKFKEMGSSVSWGKLIAASGMIAITY
                     TNREPVADVHALLRFVQQSATEPGIDASRIGLWASSGNVPLALALLMEPDFQDVQCAA
                     FCYGYTLDLNGATHVADVSRMFGFANPGQGKSMADLPRTTPLFFARAGQDQCPHLNET
                     LDQFLVDALRCNLPVSVINHPEAPHAFDLLHDSPETREIIQHILQFLRVHLLK"
     gene            1494..1973
                     /locus_tag="PKZ53_15910"
     CDS             1494..1973
                     /locus_tag="PKZ53_15910"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNJ41973.1"
                     /translation="MAKTPLKNPGVHFPPPLLFIAGMLGGWGIQQVQSAPVSAILPAA
                     VRIMTGWAIVVAGVGILAWALLTFWKIRTGIYPNQPANQIVAHGPFRFSRNPMYVAMT
                     ALTIGVSLLSNIGWILVFIPFILFALTMLVIRREEAYLTDAFGESYAQYCARVRRWI"
     gene            2013..>2204
                     /locus_tag="PKZ53_15915"
     CDS             2013..>2204
                     /locus_tag="PKZ53_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41974.1"
                     /translation="MNDIPQLQADLFRKAVRSFTDESVVYVSSPALEAFLDETNTIDL
                     PAEERLRRLAVALEYETLEH"
BASE COUNT          538 a          537 c          543 g          586 t
ORIGIN      
        1 aaggtcagga atatctggtc atggacccgc tcaacgacga caaacggctg gtcaagcttt
       61 ccgagctatc agattcgatt gaggccgttc gggtgtttcg ccagtcatag attggatgat
      121 gttgctcaaa acagaactct ttcaatgtct gatggttatc agtcaagagt caccgaactt
      181 tgataagtct tttgcttggg cagaaaaatg cctcaaatta ctcggtgagt cagatgatat
      241 tgcagatcga cttcttgacc tggtaccgcc tgaatttcca tggcaatcgc tggcagatgt
      301 acttggcatg tggatgtgga tgaccagcga taacggggca gccattttac acaccacaga
      361 aagatggcta gaagaagcaa ctgatgagcg taagtgcctg attgccttga acctggatgc
      421 ttatccgttt caaaacgcag accagatgcg agatgtgtta ctcagagtca gcttgctgag
      481 ctttgagtta cgcgaacaat gtttggtgct tatggcatca agacaaaatt gaaaacccat
      541 ttttcatcaa ggatacacca atgagccagg acacaccgca agatcatatt tcacgcaagc
      601 tcgtactctt ccagttacca gaccaggata gagtcacaat ccggcaagat attgtttttc
      661 aaacagttgg tgatgatgtt ctcaccctgg acctttatgt tccggcggat gcaaagtcgg
      721 gtgatttatt gccagccgtc atcattgtaa ccggataccc tgatgtgggg tttcagaaac
      781 tggtcggatg caaattcaag gaaatgggat cttcggtttc ctggggaaaa ttgattgcgg
      841 cttcgggaat gattgccatc acctacacca atcgcgaacc cgttgcagat gtccacgcat
      901 tgcttcgatt tgttcagcaa tcggcgactg aacctggtat tgatgccagc cggattggac
      961 tctgggcgag ttccggcaat gttcccctgg cgcttgcgct cctgatggaa cctgattttc
     1021 aagacgtcca gtgtgcggca ttctgctatg gatacaccct cgatctgaac ggtgccaccc
     1081 acgtggctga tgtttcccgg atgtttgggt tcgccaatcc aggccaggga aagtcaatgg
     1141 cggatttgcc acggacaaca cccttgtttt ttgccagagc tggtcaggac cagtgtccgc
     1201 acctgaatga aacgcttgac cagtttttag ttgacgcttt aaggtgcaat ctgccggttt
     1261 ccgtgatcaa tcacccagaa gccccgcatg cctttgattt gcttcacgat agccctgaga
     1321 ccagggagat catccagcac attctgcaat ttttgagagt tcacctgttg aaataatatt
     1381 agtctgccgt cagtagccag caaccagtag cttattaagc tctttcgaat gaagagtttg
     1441 actgttttca aacacaaagt attcattgca gaatagtata aattttgaag ccaatggcca
     1501 aaactcctct caaaaaccct ggcgttcact ttccgccacc gttgctcttt attgccggaa
     1561 tgctcggcgg atggggaatc cagcaagtac aatctgcccc agtcagcgcc atcctgccag
     1621 cagcagttcg aatcatgact ggctgggcca ttgtggttgc cggagtcggc attctggcct
     1681 gggcgttact gacgttttgg aaaatccgaa ctggcattta cccaaatcaa ccagccaatc
     1741 agattgttgc tcacgggcct ttccgcttta gccgaaatcc aatgtatgtc gcgatgaccg
     1801 cactgaccat cggggtcagc ctgttgagca atattggctg gatcctggtc tttatcccat
     1861 ttattttgtt tgcgttgacc atgctggtca tccgtcgtga agaagcctat ttgaccgatg
     1921 cgtttggtga atcctacgcc caatattgcg cacgagttcg ccgctggatc tgaccccaca
     1981 gtgacgctgt accacttcag gctgagtttt gtatgaacga cattccccaa ctccaggctg
     2041 accttttcag aaaagcagtt cgatccttta cggatgaaag tgtggtgtat gtcagcagcc
     2101 cggcacttga ggctttcctg gatgagacaa acacgattga tcttccagcc gaagaaaggt
     2161 tgcgaaggct ggcggttgcg cttgaatacg aaacgttgga acac
//
LOCUS       DAOQDO010001068         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46763_length_2200_cov_2.889977, whole genome shotgun sequence.
ACCESSION   DAOQDO010001068 DAOQDO010000000
VERSION     DAOQDO010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46763_length_2200_cov_2.889977"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..554)
                     /locus_tag="PKZ53_15920"
     CDS             complement(39..554)
                     /locus_tag="PKZ53_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41975.1"
                     /translation="MSASKSLLGKKKKTVKPQSVETPPSTDTLRSKLVDLGVAEDAEI
                     RDHSGESEKMSEVLLAFASPLLGQIRTKKDFDAALDLAILSWNMTLVSDDLRQESFDK
                     IIASYLPIYQPKIRTMIEMMIERKNTHFTDNKRFIVTYHVVGSGRSRRLSVVSKPSPS
                     ATEDQTTKNEE"
     gene            complement(684..1277)
                     /locus_tag="PKZ53_15925"
     CDS             complement(684..1277)
                     /locus_tag="PKZ53_15925"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNJ41976.1"
                     /translation="MTRKYLAGWFLGSLLVLACSGLVSADRLLGSREAREALAKLTGT
                     AYPTKQIRIRKVIPGFSSTDAIVEAEIEATFKFTRTQGQWQPTEVRIGDQQWQSLPEL
                     TAALAKAQRDHTDADLNLLATSLELYRAKQGGYVTAPDIVQLTNVLAPEYLPRLVRRD
                     GWNRQLIYQGTSGSFRLQSVGPDGKANTQDDVIIARP"
     gene            complement(1303..1869)
                     /locus_tag="PKZ53_15930"
     CDS             complement(1303..1869)
                     /locus_tag="PKZ53_15930"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNJ41977.1"
                     /translation="MYLIDGNNFARRKAQDFTYEAKRELLTQLAQFARLRRVRVCVVF
                     DGIPDRHLPDGSSFQSVKIHFAQSGNTADGYIRKLLEQSRTPRDFTIVTNDRELQMGA
                     RIRQAKVVSVPEFRAQLETAILESTALHTEAKPTTRKDDVSQWLRYFGVSADENEPEV
                     DDDQLFTREFTQPVPDRNKGKIVKKRSL"
BASE COUNT          519 a          529 c          540 g          612 t
ORIGIN      
        1 tcttcattct tcattcttca ttcttcattc ttcattcttc attcttcatt cttcgtggtt
       61 tggtcttcgg tagctgacgg cgatggcttg gaaacaacag aaagtcggcg ggaccggcct
      121 gatccgacga catgataggt gacgataaat cgcttattat cagtaaaatg agtgtttttc
      181 cgctcaatca tcatctcgat catcgtgcgt atctttggct gatagattgg caaatatgaa
      241 gcaatgatct tgtcaaatga ttcctggcgt aagtcatcag agaccagcgt catattccag
      301 ctcaaaattg ccagatcaag tgcggcgtca aaatcttttt tggttcgaat ctgacccaac
      361 aacggagagg caaatgccag caacacttca gacatttttt ccgattcacc tgaatgatca
      421 cggatttccg catcttctgc gacgccaaga tccaccagtt tactgcgaag tgtgtcggtg
      481 gaagggggtg tttcaactga ttgaggtttg actgtttttt tctttttgcc aagcaggctc
      541 tttgaagcac tcatagtgtg aaatagttct ttctattggg gttcttgctg aaaaatagct
      601 aagctttgag caattcaaca ttgagcagat acagggttgc cgctcaaaaa ttccttcatt
      661 cttcattctc aagtcttcat tcttcagggg cgggcgatga tgacatcatc ctgggtgttg
      721 gcttttccgt ctggtcctac ggattgcaac cgaaacgaac ctgaagttcc ctggtatatc
      781 aactgccggt tccatccgtc acgccgcacc aatctgggca aatattcagg tgccagaaca
      841 ttggtcaatt gcacaatatc gggtgctgtg acatagcctc cctgtttggc acgatacagt
      901 tcaaggctgg ttgccagcag gttgaggtca gcatcagtat gatcacgctg ggctttcgca
      961 agggcggcgg tgagttcggg aagagattgc cattgctggt cgccaatgcg tacttcggtt
     1021 ggttgccact ggccctgagt gcgagtgaat ttaaaggtgg cttcaatttc agcttccaca
     1081 atcgcatcgg tagatgaaaa accaggaatg accttccgaa tccgaatctg tttggttgga
     1141 taggcggttc cagtcaattt ggcgagggct tcgcgagctt cgcgggagcc aagcagccgg
     1201 tctgctgaga ccagtccact gcaggccagc accagcaggc ttccaagaaa ccatccagcc
     1261 agatattttc gtgtcataag cacttgaacc cccaaaagac acttacagtg agcgtttttt
     1321 tacaatcttt cctttgttgc gatcaggtac gggctgcgta aactcgcgtg taaacaattg
     1381 atcatcatca acttcgggct cattctcgtc agcggaaacc ccaaagtatc gcaaccactg
     1441 gctgacatca tccttgcggg ttgtaggttt ggcttcggtg tggagtgcgg tggattcgag
     1501 aatggctgtt tccagttgtg ctcgaaattc aggcacactg accactttgg cctgacgaat
     1561 gcgcgccccc atctgcaact cacgatcatt ggtcacaatg gtgaagtcac gcggtgttcg
     1621 agattgttcg agcagtttgc gaatgtatcc gtcagccgta tttccactct gggcaaagtg
     1681 gatcttgaca ctttggaatg atgatccatc aggcaaatga cgatccggta tgccatcaaa
     1741 aaccacacat acccggacgc ggcgcagacg ggcgaactga gccaactggg tgaggagttc
     1801 gcgtttggct tcgtaggtaa aatcctgtgc cttgcggcga gcaaagttat ttccgtcaat
     1861 gaggtacatg cctgaacgtg ctaagtggaa aggtgaaaac aacctgtatt gggtactggt
     1921 gcttggtatt tagtacttcg tacctggtgc ttcgtacctg gtgctttgta cttgggatcc
     1981 aataccttcc aagaaccaag aaccaagaac cagacaccag ccgcttactt taccccaagc
     2041 tgttttttga tgaaagcttc ccaggcggtc ggtgtcatct gacggctttg gcgcagacga
     2101 aatttaaagc ttttccattc cggattgtca ggcgtcgcaa tttccatgtc ttcaacatag
     2161 cctctggcct gattgaccca gataaatccg cctttgtgat
//
LOCUS       DAOQDO010001069         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46892_length_2197_cov_2.780579, whole genome shotgun sequence.
ACCESSION   DAOQDO010001069 DAOQDO010000000
VERSION     DAOQDO010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46892_length_2197_cov_2.780579"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..578
                     /locus_tag="PKZ53_15935"
     CDS             120..578
                     /locus_tag="PKZ53_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41978.1"
                     /translation="MTLSRRSFLQVTAGATLGVYLTGFEISQVTLAQSPDRFSFEVAP
                     NLDLTPEIFSQFLNTTFEVSGPGINRVPLTLVQVTDQSDHVNEVSEGRMTFTTFTLRF
                     KGARTAPLKQNTYQFNHYALGILPMFIVPGGQDASGKYFNVVYNRITGYA"
     gene            789..1292
                     /locus_tag="PKZ53_15940"
     CDS             789..1292
                     /locus_tag="PKZ53_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNJ41979.1"
                     /translation="MAEPFLSEIRIMSFVFPPKGWALCNGQLLPINQNQGLFSLLGTT
                     FGGDGRVNFGLPDLRGRVPIHVGSGHTLGERGGEQAHTLSIGELPQHTHVLYGSTQSG
                     NVFVPQSNVLAGSPNPTYSTTISALAAMSPQTVANTGGSQAHLNMQPFLTLSFCIALQ
                     GIFPSAT"
     gene            1314..1820
                     /locus_tag="PKZ53_15945"
     CDS             1314..1820
                     /locus_tag="PKZ53_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNJ41980.1"
                     /translation="MAQPYVGEIRMFAGNFAPAGWQLCEGQLMSISENETLFQLIGTT
                     YGGDGESTFALPDLRGRLPLHQGNGFILAETGGAEEITLTVQQIPAHSHPLLATVNIA
                     NNTTPVNSLPAQASTVDVYNDSASSFAAMTPQSISAVGGSQPHTNFQPYLCVNFIISL
                     YGIFPSPT"
     gene            1975..>2197
                     /locus_tag="PKZ53_15950"
     CDS             1975..>2197
                     /locus_tag="PKZ53_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNJ41981.1"
                     /translation="MADPFVAEIRIFPFNFPPKGWAWCDGQLLPLSQNTALFSLLGTT
                     YGGNGKSNFALPDLQGRAPMHPGQGPGLSL"
BASE COUNT          493 a          646 c          513 g          545 t
ORIGIN      
        1 ccacctgttc tctcccaatc gccgcttgct tcccgttcgt accatcaaca cagttgtttt
       61 cttgccccca taaacgggag ccacctggga gactgagttc gcacagttaa ggtggtttta
      121 tgactctttc ccgtcgttcg tttctgcaag ttacggctgg ggctacactc ggagtgtatc
      181 tgactggttt tgaaatcagt caggtcacgc tggcccagtc acctgaccga ttttccttcg
      241 aagtcgcccc caatctggat ttaactcccg aaatcttcag ccagtttctc aataccacat
      301 ttgaagtttc aggtccgggc atcaaccggg tccccctcac cctggttcaa gtcaccgatc
      361 aatcagacca cgtcaatgaa gtgtctgaag gccggatgac gtttacgacc tttacccttc
      421 ggttcaaggg tgcccgcaca gctccgctca aacaaaatac ctatcaattc aaccattacg
      481 cgctgggaat cttaccaatg tttattgtcc ctggcgggca ggacgcatca ggcaaatatt
      541 ttaacgtggt ttacaaccgg attaccgggt acgcctgagc ctgaagtccg cctgtatctt
      601 aaagttttca gtggtacgct gtgaccagcc tcaccagtca ttcaaggttt ttcccttctt
      661 tctttccaac atttccaggc tgttcttgtt gggaaatagt ggtacactcc gcaaccactc
      721 cgggtgcagg ttcctgcacc tctcactcaa tccagaattc aatttcatcc acaaaggtca
      781 aaaactgtat ggcagaacca tttttgtctg aaatcagaat tatgagcttc gtgtttccac
      841 ccaaagggtg ggcactgtgc aatggacagt tgctcccaat taaccagaat caggggttgt
      901 tttccctgct cggcaccacc tttggtggcg atggccgggt gaattttggc ctgccggatc
      961 tacgcggacg ggtgccaatc cacgtcggca gcggccatac gctgggcgaa cgtggcggcg
     1021 aacaggccca tacgctctcg attggggaac tcccacaaca cacccacgtg ctctatggca
     1081 gtacccagtc cggaaatgtg tttgttcctc aatccaatgt gctggccgga tcgccaaacc
     1141 cgacctactc caccacgatc tcggccctgg ccgccatgtc ccctcaaacc gtagccaaca
     1201 ccggtggaag ccaggcgcat ttgaacatgc agccattcct caccctgagc ttttgtattg
     1261 ccctgcaggg aatcttccca tcggccactt aaacacaatc aggagaccta cttatggcac
     1321 agccttatgt tggtgaaatt cgcatgtttg ccggaaattt tgcacctgct ggctggcagt
     1381 tgtgtgaagg gcaactcatg tcgatttccg aaaacgagac cctgtttcaa ctcattggaa
     1441 cgacttatgg cggggatggg gaaagcacgt ttgcacttcc ggatttacgc ggtcggcttc
     1501 cgctccacca gggcaacggc tttatcctgg ctgaaaccgg aggcgccgaa gaaattaccc
     1561 tgaccgtgca gcaaatcccg gcgcattctc acccattgct ggcaaccgtc aacatcgcca
     1621 acaacaccac gccggtcaat agccttcccg ctcaagcatc aaccgtggat gtctataatg
     1681 actcagccag cagttttgcg gctatgacgc ctcaatctat ctcagccgtc ggcgggagcc
     1741 agccccacac caattttcaa ccctatctgt gtgtgaattt tatcatttcc ctctatggga
     1801 ttttcccaag cccaacttaa ttcaggcttg ccaggggtgc gattcacttc gggatgtgaa
     1861 gcgaatcaaa ctcacaggat ctgaaccatc ggtcactgat atcaaggtct tgccggtatc
     1921 agtacttcag acttcaaccg attcttgaaa aggaaccaac gaggaccaca ttgtatggca
     1981 gacccatttg ttgctgaaat tcggatcttt ccgttcaact ttccacccaa aggctgggcc
     2041 tggtgcgacg ggcaactcct gccgctcagc cagaacaccg cgctgttctc gttgctggga
     2101 acgacctatg gcgggaacgg gaaatcaaac tttgcgctcc ctgatttgca ggggcgggca
     2161 ccgatgcacc ctggtcaggg tccaggactg tcgctcc
//
LOCUS       DAOQDO010001070         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_46960_length_2195_cov_2.711682, whole genome shotgun sequence.
ACCESSION   DAOQDO010001070 DAOQDO010000000
VERSION     DAOQDO010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46960_length_2195_cov_2.711682"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..>2195
                     /locus_tag="PKZ53_15955"
     CDS             361..>2195
                     /locus_tag="PKZ53_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ41982.1"
                     /translation="MAHVSRDQKLPLSPLQQMFWMLDKLKPASSFDHAPMNVTYNRRF
                     RGQFKVEVLERCFSEIIRRHEALRTTFVAVDGQPVQIIHPASEFRFALLDQVPADFRL
                     KPVNDNVNILVDLSNLPATEHAHAIEAIVTTEAQRLFDISVGPLLRVVVLRLSPEDHV
                     LLLNIHHIVSDGWSVNVLIQELSALYNAFGAGQPSPLPALPIQYADFACWQQQWIAEH
                     KLDDGIKYWKDQLTGAPPLLTLPTDFPRPAVQSFRGARETIEIPQEMYAALKGLSQSQ
                     GATLFMTVLAAFQTLLSKYARQEDIVVGTPIANRDLFETKRLIGFFTNVLALRTDVSG
                     DPTFAELLGRVREMTIAAFTHQNVPFQKVIEAVQPQRDLSYPPIFQVMLVLQNTVKQA
                     ISLENLTVEIIDADTGTARNDLTFELLDIGNRISGVVEYNTSLFTAGTIQRMISQFHA
                     LLEHIIEDPNRPLSGLSLLSSQEVNLLAQWNQTETAYPRHLKAHELFETQARQNPDAT
                     ALVYAEKDEAGRFKEIRLTYAEVNTRAEQLAHYLRDQDVTPYTRVGLCLAPSVELLVG
                     MLAILKAGGAFVPLDPAYPQTRLEFMIRDARLPVILTHSTLVEALP"
BASE COUNT          519 a          635 c          540 g          501 t
ORIGIN      
        1 caccggagca ttccaattcc attgatacca gtcagtaaaa accagggctt ggggcttggg
       61 gctgaagaaa tcgggctcag ggttcaagaa aatgagccac tcgcatctcg cactccaaag
      121 gagcagcaca atggtagcct gggggaagct gctctgagcg cactcccagg gatagatccc
      181 caccttgatt ctgccccggc aggggctgga atgacgaatg ccctttcagg gcaaaaagag
      241 aaagagggtg acgaaacctg gggtgcaaag ccaccccagg ctaccactct ggctacctct
      301 ttgaggtgcg accaaaattc tgattctgaa acacttgctc ctgaactgca ccctctcgct
      361 ttggcacatg tctcccgtga ccagaaactg ccgctttcgc cactccagca aatgttctgg
      421 atgcttgata aactcaagcc ggcgagtagt ttcgaccacg ctccgatgaa tgtgacgtat
      481 aaccgccggt ttcggggaca attcaaggtt gaggttctcg aacgctgttt ttctgagatc
      541 atccgacgac acgaagcgct gcgcaccacg tttgtggctg ttgacgggca gccggttcag
      601 atcattcatc cggcctctga atttcggttt gcgctgcttg atcaggtccc ggctgatttc
      661 cggttaaagc cagtcaacga caatgtgaat atactggttg atctttcaaa ccttccagcc
      721 accgaacacg cccacgccat tgaagccatt gtcacaactg aagcccagcg actgtttgat
      781 atttcagtgg gaccgttact tcgggttgtc gttctgcgac tctcacccga agatcacgtg
      841 ctgctgttga atatccacca catcgtgagt gatggatggt cggtaaatgt gttgattcag
      901 gaactttcag cactctacaa cgcctttggt gccggtcaac cttcgccctt gccggccttg
      961 ccaatccagt atgcggactt tgcctgctgg cagcagcaat ggattgccga acacaaactg
     1021 gatgacggca tcaaatactg gaaagaccag ttaaccggag caccgcccct gctcacactg
     1081 ccaactgatt ttccgcgtcc ggcagtgcaa tcgttccggg gtgcgcgtga aacgattgaa
     1141 attccccagg aaatgtatgc cgcactcaaa gggctgagtc agtcacaggg cgccaccctg
     1201 tttatgacgg tgctggcagc attccaaacg ctgctgtcca aatatgcccg ccaggaagat
     1261 atcgtcgtag gaaccccgat tgccaaccgc gatctctttg aaaccaaacg cttaattggc
     1321 ttttttacca atgtgctcgc cctgcgaaca gatgtgtcag gcgacccaac ctttgctgaa
     1381 ctccttggcc gggttcggga aatgacgatt gccgccttta cccatcaaaa tgtcccgttc
     1441 caaaaagtca tcgaagccgt tcaaccccag cgtgatttga gctacccacc gattttccag
     1501 gtcatgctgg tgctgcaaaa caccgtgaaa caggcgattt cacttgaaaa cctgacggtt
     1561 gaaatcattg atgccgacac cggaaccgcc cgcaacgatc tgacctttga actgctcgat
     1621 attggcaacc ggatttccgg cgtggtcgaa tacaacacga gcctgttcac cgctggaacc
     1681 atccagcgta tgatcagtca gtttcatgcg ttgctggaac acatcattga ggatcccaac
     1741 cgaccactct ctggactctc acttctttca tcccaggagg tgaacctcct ggcacagtgg
     1801 aatcaaaccg agacggctta tccgcgacat ctcaaagccc acgaactctt tgaaacccag
     1861 gcccgccaaa atcccgatgc tacggcgctc gtctatgccg aaaaagatga agcgggccgt
     1921 ttcaaggaaa tcaggctaac ctatgcggaa gtcaacaccc gtgctgagca gttggcccac
     1981 tacctgcgcg atcaggatgt cacgccgtac acccgtgtcg gcctgtgcct ggcaccatcg
     2041 gttgaattac tggttggcat gctcgcgatt ttgaaagccg gaggcgcgtt tgttccgctt
     2101 gatccagcct acccacagac gcggctggaa tttatgatcc gcgacgcccg gttgccagtc
     2161 atcttgactc attcaacact cgttgaagcc ttacc
//
LOCUS       DAOQDO010001071         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47003_length_2194_cov_2.465171, whole genome shotgun sequence.
ACCESSION   DAOQDO010001071 DAOQDO010000000
VERSION     DAOQDO010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47003_length_2194_cov_2.465171"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..>2194)
                     /locus_tag="PKZ53_15960"
     CDS             complement(70..>2194)
                     /locus_tag="PKZ53_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ41983.1"
                     /translation="SQFEPAIKDFDEAIKLKPAYALAFYNRGFTYDDQGKYDLAIKDY
                     DQAQKLDPKLVQVYFSRGVAYNALGKDELAIKDFDETLKRNPKEASALVYRGFSYNNL
                     GKYEQAILDFDAALKINPNLSAAYAYRGYARSNLSQNDKAVADFTQAIKLNPKDVSAF
                     TGRSVCYIRQLKYTEALADCDAAIRLNENNARAYGNKGLALLGLKRDDEARQAFDRCY
                     TLNPALKESYEKSILAFRPNGLDGETKTAETTPPPVREERPEPTREEDRTPRVSRGEQ
                     AFLNGKDLAKQDKHREAIDQFSEAIDRNYRTVDVYLARGQSYGRLNDWEAALKDFDEV
                     VRLDSRNAQGYYNRGRVLTTLKRTQPALDDFDQAIRYGSNDPDLQGKAFYRRGKINYE
                     LSQYQKSIDDCTDAIRLLPESVDAYNVRGLARFELEDYELAVSDFSEAIRREKSFAEL
                     YGNRGVANLMLNRDRDAQDDLARCYELEPDLRSTYEPLAEDARSKRRTVASSSPPPRT
                     TSGNPSRSTSNPPSRSTPPVRTNPSTSQPPPTRSTNPASGGNSRALLEAARGGHNASV
                     AALVRAGADPNSIDENDRPAITLAAENGHNDTVKELLIQGADPNLKDANGMTATMYAA
                     RGGHDATVGALVKRNADVNAQDNQGRTALMFASMGGHSNTVRALLTARAQVNVADNNG
                     QTALGYARSFGHSQTAQLLISANAK"
BASE COUNT          454 a          606 c          574 g          560 t
ORIGIN      
        1 caccggtctc aattcagcca ccgctacgcg gcgaagaaat gtcaacagaa cctagacacc
       61 tgactggaat tatttcgcat tcgcgctgat cagcaattgc gcggtttgcg aatgaccgaa
      121 cgaccgggca tatccaagcg ctgtctggcc attattgtca gcgacattga cctgggcccg
      181 ggcagttaac agtgcccgaa ctgtatttga atgcccaccc attgaagcaa acattaacgc
      241 ggtccggccc tggttatcct gggcgttgac atcggcattg cgtttcacca gcgcgccaac
      301 cgtagcatca tgtccacctc gcgccgcata catggtggcg gtcattccgt tcgcatcctt
      361 gagatttggg tcagctcctt gaatcaaaag ttctttgacc gtgtcgttgt ggccgttttc
      421 cgccgccagg gtaatggctg ggcggtcgtt ttcatcaatg ctgtttgggt cggcaccggc
      481 tcgaactaag gcggcaaccg aggcattgtg tccacctctg gcggcttcca acaaagcacg
      541 cgagttgcca ccagaagctg gattcgttga acgggttggc ggaggctggc tcgttgacgg
      601 attggtccga actggaggcg tcgagcgtga aggcggattt gaggttgatc tggacgggtt
      661 tcctgaagta gttcttggag gcggtgacga actggccacc gtgcgccgtt tggaccgggc
      721 atcctcggcc agcggttcat acgtgcttct gaggtctggc tccaattcat aacatctggc
      781 cagatcgtcc tgcgcatcac gatcccgatt cagcatcagg ttggccacgc cacggtttcc
      841 atagagttcg gcaaagcttt tctcacgtcg gatggcttcg gaaaaatcgc tcacggccag
      901 ctcataatcc tccagttcaa atctggccag cccgcgcacg ttgtaggcat ccaccgattc
      961 cggcaacaac cggatggcat cggtacaatc atcaattgat ttctgatact ggctgagttc
     1021 atagttgatt ttgccgcgcc gataaaaggc tttcccctgc aaatccgggt catttgaacc
     1081 ataccgaatt gcctggtcaa aatcatccaa cgccggctgt gtgcgcttta atgtggttaa
     1141 gactcgcccc cggttgtaat acccttgcgc attccgactg tcgagccgga caacttcgtc
     1201 aaagtccttg agcgccgcct cccagtcatt gagccggcca tatgactgac cgcgcgccag
     1261 atagacgtca acggtccggt aattacggtc gattgcctca gaaaactggt ctattgcttc
     1321 gcggtgtttg tcctgttttg ccagatcctt tccattgaga aatgcctgtt cgccacggct
     1381 cacgcgtggc gtgcgatctt cctcgcgggt aggctcaggc cgctcttccc gaactggcgg
     1441 cggggtggtt tcagccgttt tggtctcacc atctaatcca tttggacgaa aagccaaaat
     1501 tgatttttcg taactttcct tgagcgccgg attgagcgtg taacaccggt caaatgcctg
     1561 tctggcttca tcatcccgtt ttaacccaag cagcgccaat cctttattcc catatgcccg
     1621 ggcgttgttt tcattgagcc ggatggcggc atcacaatca gcgagggctt cggtgtactt
     1681 taactggcgg atataacaaa cgctgcgacc ggtgaaggca gaaacatcct ttggattgag
     1741 cttgatcgcc tgggtgaaat cggctacggc tttatcattc tggctcagat tgctgcgcgc
     1801 atatccccgg taagcatagg cagcactcag gttcgggttg atctttaacg ccgcgtcaaa
     1861 gtccaggata gcctgctcat acttgcccag gttgttatat gaaaatccac ggtagaccag
     1921 tgcggaggct tcttttggat tccgcttcag ggtttcatca aagtctttaa tcgccagttc
     1981 gtctttccca agcgcgttgt aggccacgcc ccggctgaaa tatacctgaa ccagcttcgg
     2041 atccagcttc tgggcctggt catagtcttt gatcgccagg tcatattttc cctgatcatc
     2101 ataggtaaac ccacgattat aaaaggccag cgcgtacgcc ggtttgagtt tgatggcttc
     2161 gtcaaaatcc ttaatcgcgg gctcaaattg cgac
//
LOCUS       DAOQDO010001072         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47185_length_2189_cov_2.991097, whole genome shotgun sequence.
ACCESSION   DAOQDO010001072 DAOQDO010000000
VERSION     DAOQDO010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47185_length_2189_cov_2.991097"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..863)
                     /locus_tag="PKZ53_15965"
     CDS             complement(270..863)
                     /locus_tag="PKZ53_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41984.1"
                     /translation="MNYFDIHQTGQFINPFKEPDLHTNESDWQIARQLVPGMMSYIFG
                     PENETEPLQVWLKKRLVREGLEVLIIMLRKRLLSPDYLPSVQMHIDPENGKGRSCTEQ
                     IEQMCQDKPIEQKKRILSPAVYLMKRLITEAAERPNTFFGTQKISLNDPRVHEVIAAV
                     ALSIIQIQSTAGVFTQEITGASEDAVNKITSVLHNPV"
     gene            1120..>2189
                     /locus_tag="PKZ53_15970"
     CDS             1120..>2189
                     /locus_tag="PKZ53_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41985.1"
                     /translation="MSLSLFHLLGWLMVGCVAFVGSGWSSQSETLQKPLPEPAPVVQT
                     QPEPQLPEKPPRWKWDYTFRNRTGLKLNQPHVVQLSRTIVDVKGVCEFNPTWRLTVEG
                     RASLDPVQRLGYPTGVWVEPRQVFVEGTLKNVGLKLGLQQLVWGQADGLRVLDVINPL
                     DYREFLLDDFLDSRRPLWMARADVPIGAGSIQVAWVPYFAPGRLPVNTDEFGFGKSFG
                     LGLFEAQAALTGTIATRVEPTRRPGYRLSSSQIGLRYRQTLGNWDITANFFHGWEDVP
                     TPYPKGFEFSPQPTVVFEPNFDRKTVLGGTGATTFGPVVLRLEAGWNFNRSQFLRKSA
                     TDLGFEKKGQFSSVVGLDYSPRP"
BASE COUNT          525 a          548 c          545 g          571 t
ORIGIN      
        1 aaaccattga agaatttgga tttattttct gtgtattccg tgtattccgt ggttttttta
       61 tctgaaattc tatagtctca ggttgcaccc tggactcaaa accaagattc aataccaaat
      121 cctcgttgta gtattaattc gcatactaat ccctactcaa gagacatcac ccagacagag
      181 aagttaaaaa gatcaaaagg cttgaaaatt tgttgatttc tcaagaaatt tccaagcctc
      241 agatatttca caaagttgca acccttgttt cagaccgggt tgtgcaacac tgaagtgatt
      301 ttgttgacgg catcttcact ggccccggta atttcctggg taaagacgcc agccgttgat
      361 tgaatctgaa tgatagacag cgcaacggcg gcaatcactt catgcacccg aggatcattc
      421 aacgagattt tttgagtacc gaagaaggta tttgggcgtt ctgcggcttc agtgatcagg
      481 cgtttcatca ggtacaccgc cgggctcaga attcgttttt tctgttcaat cggcttgtcc
      541 tggcacatct gctcgatctg ttcggtacag gaacgacctt ttccattctc agggtcaatg
      601 tgcatttgca ctgaaggcaa atagtcaggg gagagcaacc gcttgcgaag catgatgatg
      661 aggacttcaa gcccttctcg caccagccgc tttttaagcc agacctgaag cggttcagtc
      721 tcattttccg gtccaaaaat gtaggacatc atgcccggaa cgagctggcg tgcaatttgc
      781 cagtccgact cattcgtgtg caaatccggc tctttgaagg ggttgataaa ttgtccggtt
      841 tgatggatgt caaaataatt catgtgacga cctttagaac gagaaagaat gcccggtgta
      901 aggggtggca ggataatcag acggaaggag cgtgacgctg tatttttatc ttctcccaac
      961 tggaaatttc cggcaaggac ttttcacaaa attctaccca actctttgaa atcagttgac
     1021 tctgacctga gagatcacaa accaggtgtg aaaaactgaa gtgctggtat atgctcgcgt
     1081 gtttgttcaa accttttgaa tcggcgagtt ttctccgcta tgtcgctttc tttgttccac
     1141 ttactgggct ggctgatggt tgggtgtgtt gcctttgttg ggtcaggatg gtcatctcaa
     1201 tctgaaacct tgcaaaagcc tctacctgaa cctgccccag ttgtacaaac ccagccggag
     1261 cctcaactac ctgaaaaacc accacgctgg aaatgggact ataccttccg caatcgaacg
     1321 ggcctcaagc tcaaccagcc acacgtggtg caactttcgc ggacaattgt tgatgtcaaa
     1381 ggcgtgtgcg aatttaaccc gacctggaga ctcacggttg aaggccgggc cagtctggat
     1441 ccggtccaac ggctgggcta tccaacggga gtttgggtcg aaccacgcca ggtatttgtg
     1501 gaaggcaccc tcaaaaacgt cggtctcaaa ctgggcctcc agcaactggt gtggggtcag
     1561 gcagatggat tgcgggtcct ggatgtgatc aacccacttg attaccgcga atttctcctg
     1621 gatgactttc tggattcacg ccgcccattg tggatggcac gggctgacgt tccgattggc
     1681 gccgggtcga tccaggtcgc ctgggttccc tatttcgctc cgggacggct gccagtcaac
     1741 accgacgaat tcgggtttgg aaaatcgttt ggacttggcc tttttgaagc ccaggcagca
     1801 ttgaccggaa ccatcgctac cagggttgag cccacccgcc gccctgggta tcggctgagt
     1861 tcaagtcaga ttggactccg ctatcgccag acgcttggaa actgggacat caccgcaaat
     1921 ttctttcacg ggtgggaaga tgtcccgacg ccatacccca aaggatttga attttcaccc
     1981 caaccaaccg tggtgtttga accgaacttt gaccgcaaaa ccgtgcttgg cgggacgggt
     2041 gccacgacgt ttggaccggt ggttctccga cttgaagccg gctggaactt caaccgttcc
     2101 caatttctga gaaaatccgc cactgacctt gggtttgaga aaaaaggaca attttccagc
     2161 gtcgttggcc tggattattc accccggcc
//
LOCUS       DAOQDO010001073         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47200_length_2189_cov_2.160262, whole genome shotgun sequence.
ACCESSION   DAOQDO010001073 DAOQDO010000000
VERSION     DAOQDO010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47200_length_2189_cov_2.160262"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..368
                     /locus_tag="PKZ53_15975"
     CDS             6..368
                     /locus_tag="PKZ53_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41986.1"
                     /translation="MRLSPPTQKEVTVKLGQEVVIDGEKLRLEFSQLLGDSRCPVDVD
                     CIWQGNAEITVGIGAINKRKRIGEYFKLNTALEPQEVKYKNYTIKFVKLSPDPKAGVV
                     IDPSQYEATLLITNQRSK"
     gene            585..>2189
                     /locus_tag="PKZ53_15980"
     CDS             585..>2189
                     /locus_tag="PKZ53_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41987.1"
                     /translation="MKIGRVQFGPGAVILMIGVGLGLVYFGLDRLGLLQSLKSKINPV
                     GALPVGSVVLDPREELPTDVKDLPASQVKPPERTSDNPEVTIGIWTWQTTSGIIDAVG
                     GPGKSGDYSGSCLCQAGITNTRLIVQNDTSEQIKALAAGQLQFVTTTGDQAAVDIAGA
                     NKLLRGNKARVVWSSGYSFGEDCLMGPASWKADPQKARGAVVVTAVPYCDWNVTVDWA
                     ADNQIPINPDESVYDPTAVNFVNATDHIEAAQKFVKNTNVSLKNRQTGQPEEHQIDAV
                     ATWTPGDVMAFEGRSTVTYQGAAEKLQKIVSTKEYSYMMPHILFGNADWINQHRDYVQ
                     TLLRCIARSNERIKADDEYLKTRVATLNSVIFNLDGRGPNFWYKYFHGATENGVPLGG
                     SRVNNIAEVRHLFGLENYQPIERSIFGITYLDHAKRLQKLMPQRLEGFTPVAQVVDLS
                     FIQEMTDERSLTPVYQAQFPAGTPNPGTVVKANYQINFDSGSARIKPSELAVLQAIRN
                     LLIRASDTQITIEGHTDNTGDDNTNLR"
BASE COUNT          578 a          495 c          563 g          553 t
ORIGIN      
        1 tggcaatccg actgtcaccg ccgacccaaa aagaagtcac tgtcaaactg ggccaggaag
       61 tggtgattga tggtgaaaaa ctcaggcttg aattttctca actgctgggc gatagccggt
      121 gtccagttga tgttgactgt atctggcagg gaaatgctga aattacagtc ggcattgggg
      181 cgatcaataa acggaagcga atcggtgaat atttcaaact caacactgcg cttgaaccgc
      241 aggaagtgaa gtacaaaaac tacaccatta agtttgtcaa attgagccca gatccaaagg
      301 ccggagttgt gattgaccct tcccaatatg aagccacgtt gctgattacc aaccagcggt
      361 ccaaataacc tggtttgttt ctccttgtcg aaggctgaac caatgacact gtgtgattgg
      421 ttcagctttt tttatgcctc gaatcaggtc caaactccgg tcgaacgtac accgttctgt
      481 acaatgattc caaatacggt ttttgttgca attttttcct gacaggtata gattctggag
      541 cgtcctcctt gccctgatgt tttcgaataa aggggctcac ggctatgaaa atcggtcgtg
      601 ttcaatttgg tcctggagct gttattttga tgattggtgt cggtttaggg ctggtttact
      661 ttggcctgga ccgattggga ttgttgcaaa gcttaaaaag taaaataaat ccggtcgggg
      721 ctcttcctgt tggctcggtt gtgcttgatc cacgtgaaga acttccaact gatgtgaaag
      781 atttgcctgc atctcaggta aaaccgccag aacgaaccag cgataatcca gaagttacaa
      841 ttgggatttg gacctggcaa accaccagcg gcattattga tgccgtcggc ggacctggaa
      901 aatcgggaga ttattcagga agctgtctgt gtcaggctgg tattaccaat acccggttga
      961 ttgttcaaaa tgatacgtct gaacaaatca aagctctggc tgccgggcaa ttgcaatttg
     1021 tcaccacaac gggcgatcag gctgccgttg acatcgctgg ggcaaacaaa ttgctgcgtg
     1081 gcaacaaagc cagagtcgtt tggagcagtg ggtattcatt tggtgaagat tgtttgatgg
     1141 ggccggcttc gtggaaagca gatccccaaa aagcgcgcgg tgcggtggtg gtcacggcgg
     1201 tgccatattg tgactggaac gtaacggttg attgggcggc tgacaatcag attccgatca
     1261 acccggatga aagtgtgtat gacccaacgg cggtcaactt tgtcaatgcg accgatcaca
     1321 tcgaggcggc ccagaaattt gtcaaaaaca ccaacgtcag tctcaaaaac cggcaaaccg
     1381 gccagccgga agagcaccag attgatgcgg ttgcgacctg gacgccgggt gatgtgatgg
     1441 catttgaagg acgatcaacc gttacctacc agggcgctgc tgaaaagctg caaaagatag
     1501 tttcaaccaa agaatatagc tacatgatgc ctcatattct gtttggcaat gctgactgga
     1561 tcaatcagca tcgtgactat gttcaaacac ttctgcggtg tattgcccgt tcgaacgagc
     1621 gaatcaaagc cgatgatgag tacctcaaaa cccgtgttgc cacattgaat tcagtgattt
     1681 tcaatctgga cggacgtggg ccaaacttct ggtacaaata ttttcatggt gcgactgaaa
     1741 acggggtgcc gttgggtgga tcacgggtga acaatattgc tgaggtacgc catttgtttg
     1801 gccttgaaaa ctatcagccg attgagcgca gcattttcgg tattacatat cttgaccatg
     1861 ccaaacggct gcaaaagctg atgccacaac ggcttgaagg ctttacaccg gttgctcagg
     1921 ttgtggacct tagttttatt caggaaatga ctgacgaacg aagtttgaca cccgtctatc
     1981 aggcccaatt tccggctgga actcccaatc cggggaccgt cgtcaaagcc aattaccaga
     2041 tcaactttga ttcaggctct gccaggatca aaccgtctga actggctgtc ctgcaagcga
     2101 ttcgtaactt gctgattcgg gcttcggaca cccagattac aattgaaggc cacacagaca
     2161 atacaggtga tgacaataca aatttgcgg
//
LOCUS       DAOQDO010001074         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47222_length_2188_cov_3.489920, whole genome shotgun sequence.
ACCESSION   DAOQDO010001074 DAOQDO010000000
VERSION     DAOQDO010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47222_length_2188_cov_3.489920"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..517)
                     /gene="rpsG"
                     /locus_tag="PKZ53_15985"
     CDS             complement(44..517)
                     /gene="rpsG"
                     /locus_tag="PKZ53_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNJ41988.1"
                     /translation="MPRRRVAERREVLPDPVYNSEMVTKFINGLMWQGKKSVAEKIFY
                     SALEDVRQKTGDDPLKVFKKALDNVRPRVEVRSRRVGGATYQVPVEVDQRRRGTALAI
                     RWLVNYSRSRGEKTMMGRLSGELLEASNGRGNAVKKREETHRMAEANKAFAHYRW"
     gene            complement(604..1038)
                     /gene="rpsL"
                     /locus_tag="PKZ53_15990"
     CDS             complement(604..1038)
                     /gene="rpsL"
                     /locus_tag="PKZ53_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNJ41989.1"
                     /translation="MPTINQLVRKGRSQVKYKTSSPALQGCPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGISHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DSDGVKNRMQGRSKYGAKRPKEGAKGAAGKGAPVKGGPAKKK"
     gene            complement(1721..>2188)
                     /locus_tag="PKZ53_15995"
     CDS             complement(1721..>2188)
                     /locus_tag="PKZ53_15995"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ41990.1"
                     /translation="GGSQVHGRITGNVYAELHAQLKKRPCTPFNTEIPIKTPMLPPYR
                     YPDASVACGNPVFEKFLGIDLLTNPVLVVEVLSPHTEAVDRKEKFEAYKAIETVREYL
                     LIAQNAPLITHYVKQENNEWKRFDTADLTATLTLNAIDCTLAVSDVYAGVEFQ"
BASE COUNT          503 a          559 c          553 g          573 t
ORIGIN      
        1 ggtggcctcc aaaactcaat gtccaaaagg gttagacttg ggattaccac cggtaatggg
       61 caaatgcctt gttggcttcg gccatacggt gggtttcctc acgcttcttg acggcatttc
      121 cacggccatt tgaagcttcg agcagttcgc cagacaggcg acccatcatt gtcttttcgc
      181 cgcgggagcg agaatagttc accagccagc ggatcgcgag tgccgttcca cggcggcgct
      241 gatcaacttc gaccggcacc tggtaggtcg caccgccaac gcggcgtgat cggacttcaa
      301 cccgtggacg gacgttgtcg agcgcttttt tgaagacctt gagcgggtcg tcgccggtct
      361 tttggcggac atcctcaagc gcgctgtaga aaatcttctc agcaaccgac ttttttcctt
      421 gccacatcag gccgttgata aacttggtta ccatctcgct gttgtacact ggatcaggta
      481 gaacctcacg tcgctcagct actcttcgtc ttggcatatg cttcttcttc tactgaattt
      541 ctcaagatta aactccaaac ccggtctgga tttggccaac acaaactcgt acagcgtgca
      601 agcctacttc ttcttggctg gtccgccctt gactggtgcg cctttgccgg cggcaccctt
      661 ggcgccttcc ttcggacgct tggcaccgta ttttgaacgg ccctgcatcc ggttcttaac
      721 cccatcggag tcgagcgttc cacgcaccac gtgatagcgc acaccgggga ggtccttcac
      781 acgaccaccg cgaatcaaaa cgattgagtg ttcctgcagg ttgtggctaa tcccgggaat
      841 gtaggtcgtg acttcgattc cattggtcag acggacacgg gcgactttcc gcaaagctga
      901 gtttggtttc tttggcgttt gcgtgtacac gcgggtgcac acaccgcgct tttgcgggca
      961 gccttgcagc gcagggctgc tggttttgta tttaacttgt gaacgacctt ttcgaaccag
     1021 ttgattaatg gtaggcacgc gaacgtctcc ttagaacaca caacaaaata aattccaaca
     1081 ccagaagaac aggaactgct ctcctggtca atgctttgcc cgaggctcaa agcaaccaaa
     1141 agcgatacaa ggcaatagat tgcccttggc tgccaagcca gttctctttc gactaaactg
     1201 agttcaattg tggtttgggg atgttctgga agcacggagg ctcgatgcag atacagttga
     1261 ggcgtcccca acgccaggtt tttctgtggc gtgcgtaaat ggaagcttga gtcaagcttt
     1321 aattgaaggg aaagcagaac cgaaattcgg ttgctttaca agtgccagat gacgtgtggt
     1381 taccgcatct gttttacgcc acaatatatt accttcaatg aacacttcgc caccggaagc
     1441 cgcttcccga aggatttggt ttcgtgtgag tacctctgca tctcagaact gagttgagta
     1501 ctgactccca ggagaattct gggcgcttga cgaaaccgtg agtgtatgga tggaaggtta
     1561 gtctgtcaac gacttttttt ggattttttc cgggataggg aaagaaaatt cttgaatatg
     1621 aagaatttgg ggtcaaatct tcggtcttaa atcgcttttg cagggcaagg gttcagattc
     1681 gcgcaaaata caaaatacac aagatcaacc cgatttgcct ctattgaaac tcaaccccgg
     1741 cgtacacatc actgactgcc agagtgcagt caatcgcgtt cagggtcaaa gttgcggtga
     1801 ggtccgccgt gtcaaatcgt ttccactcgt tgttttcttg tttgacataa tgcgtgatga
     1861 gcggtgcgtt ttgggcaatc agcaggtatt cccgaaccgt ttcaattgcc ttgtacgctt
     1921 caaatttttc tttccggtcc acagcctccg tgtgtggaga taaaacctca accaccaaaa
     1981 ccgggttggt caagagatca atccccagaa atttttcaaa aacaggatta ccacaggcaa
     2041 cactggcatc cgggtagcga tagggtggaa gcattggggt tttaattgga atctcagtgt
     2101 taaacggcgt acagggacgc ttttttaatt gggcgtgtaa ttcagcatac acattgcctg
     2161 tgatacgccc atgaacctgg cttccgcc
//
LOCUS       DAOQDO010001075         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47282_length_2187_cov_2.492026, whole genome shotgun sequence.
ACCESSION   DAOQDO010001075 DAOQDO010000000
VERSION     DAOQDO010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47282_length_2187_cov_2.492026"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKZ53_16000"
     CDS             complement(<1..667)
                     /locus_tag="PKZ53_16000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ41991.1"
                     /translation="MAKNKYPTSVSSARLSALSALIEPPVTGHEPIQDLAVSTLRENP
                     FQPRTNHSADSLDDLAQSIKEHGLLSPLLARAESDGTFTVIAGHRRWLAVQKLGLKTV
                     PVVVRKATDAQLQLLALIENIQREDLHVIDKAQAFWRIADQFPTQEKAAEALGVKRDA
                     LAQWLRTRDLEAEILTICGTIPNLSLRTLLRLVALPPRQRLTEAKRLAALQSPKPASA
                     TTEP"
     gene            complement(660..1481)
                     /locus_tag="PKZ53_16005"
     CDS             complement(660..1481)
                     /locus_tag="PKZ53_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ41992.1"
                     /translation="MLIAIFNQAGGVGKTTLTRDLGYELSLRNQRVLVIDADPQGTLG
                     SFLGFDSQILPEDQTFWKSVVSDADLLPHIISTPFNLALGVCNRMMIAGEIQLMQEKN
                     PARFRGILSQIRSEFDVILVDCPPRVSEITVQVLLAADGLLIPVQTEFKAVSSFAEVQ
                     VEISKTQRRRRDMGLGALRVLGVVPTLFDTRLKVHSFHLDELRTQICPEFHYQVLSPF
                     RKYKSVIESGTFRKPLRVFDKNCPANEDVAAIAEAVLESLNPHKQASRHTQEAHG"
     gene            1773..>2187
                     /locus_tag="PKZ53_16010"
     CDS             1773..>2187
                     /locus_tag="PKZ53_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41993.1"
                     /translation="MSHRASVRLCLGLATALVIQFSLSIFGQLLPEQISRESARLKQI
                     ATEIGYEDKADQDNLLQEIETLNLATQKGLEHYALHRLYRVWPMTSAYEAMKVNDKID
                     NKDLDGFEKHWKAAGVELTEQEKQASLKKLATQPLL"
BASE COUNT          521 a          553 c          608 g          505 t
ORIGIN      
        1 gtggttcagt cgtcgcagag gctggttttg gtgactggag tgctgccaga cgcttcgctt
       61 cggtcagccg ttgacgcggc ggcaacgcaa ccagtcggag cagggttcgg agcgacaaat
      121 ttggaattgt cccacagatt gtcagaattt ctgcttccag gtcacgagtt cggagccatt
      181 gcgccagcgc gtcacgcttg acgcccaggg cttcggcggc tttttcctga gtcggaaact
      241 ggtcggcaat gcgccagaac gcctgcgctt tgtctatgac gtgcaggtct tcgcgttgaa
      301 tattttcaat cagggccagc aattgcagtt gagcgtccgt tgccttgcgc accacaaccg
      361 gaaccgtttt caagcccagt ttttgaactg ccagccagcg ccgatgaccg gcgatgacag
      421 tgaaggtacc atccgactca gcacgggcca gaagcggcga tagcaatcca tgttctttga
      481 tggattgggc caggtcatcc agcgagtctg ccgaatggtt tgtccggggc tggaatggat
      541 tttcgcgaag cgttgaaaca gcaagatcct ggattggttc gtggccggtg acgggcggct
      601 caatcaaggc cgacagggcc gacaaccgcg ctgaagacac cgacgtgggg tatttattct
      661 tagccatgag cttcctgtgt gtggcgtgat gcttgtttgt gagggttgag cgactcgagc
      721 actgcttcgg caatcgccgc cacatcctcg ttggccgggc aatttttatc aaagacccgg
      781 agcggttttc gaaacgtgcc cgattcgatc acgcttttat attttctgaa gggcgacagg
      841 acctgataat gaaactccgg acagatttgg gttctgagtt catcaagatg aaatgagtga
      901 accttgagcc gggtatcaaa cagggttggg acgactccaa gcactctgag cgcgcccaga
      961 cccatatcgc gccgtcgccg ctgggtcttg gagatttcaa cctggacttc ggcaaacgat
     1021 gagacggctt taaattcggt ctggactgga atcaacaacc catctgccgc cagcaacacc
     1081 tggacggtaa tttccgaaac acgtggtggg caatccacca atatcacgtc aaattcagaa
     1141 cggatttggg acaaaatgcc gcgaaaccgg gccgggttct tttcctgcat gagttgaatt
     1201 tcacccgcaa tcatcatccg attgcacaca ccgagcgcca ggttaaacgg cgttgagatg
     1261 atgtgtggga gcaggtcggc gtcggaaacc actgatttcc aaaaggtctg gtcttcgggc
     1321 agaatctggg aatcaaaccc caaaaatgaa ccaagtgtgc cctgcggatc ggcatcaatg
     1381 accagaacgc gctgattccg caaactgagt tcatatccca aatcgcgggt cagcgtggtt
     1441 ttgccgacac ctcccgcctg gttaaaaatc gcaatcagca tgggttgtat ccttacagtt
     1501 tggttcgatt ggaagagaag gtcggggaac gctcgacgtt accttctttg acaggcttga
     1561 agcaagctga ttgaatgcga aagtggcgtc aagccgccgc actccacagt tgaactgttc
     1621 ggctttgaga cgacaggtgc gcgaaaatga acaattgaag ctggttaaac ttgaggaagc
     1681 gaccgttctg gtcgggtcaa aaaggatttt caggtctggc acgctgcttt ctatagtgga
     1741 ccttccacct tttcaatcca gaggaacctc tcatgtctca ccgcgcttcg gtacgactgt
     1801 gccttggtct cgcaacggcc ctggtgattc agttctcact ttcgattttc ggacaactgc
     1861 tgccagagca gatttccagg gaatcggccc ggctcaaaca aatcgccact gaaattggat
     1921 atgaagataa ggccgatcag gacaatctgt tgcaggagat tgaaaccctg aacctcgcaa
     1981 ctcaaaaagg tctggagcac tatgcgctgc atcggttgta ccgggtctgg ccgatgactt
     2041 ctgcctatga agccatgaag gtcaatgaca agattgataa caaagacctg gatggatttg
     2101 aaaaacattg gaaagctgcc ggtgttgaat tgaccgagca ggaaaaacag gcttcattga
     2161 aaaagctggc aacccagccg ctcctgg
//
LOCUS       DAOQDO010001076         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47384_length_2184_cov_2.820103, whole genome shotgun sequence.
ACCESSION   DAOQDO010001076 DAOQDO010000000
VERSION     DAOQDO010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47384_length_2184_cov_2.820103"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKZ53_16015"
     CDS             complement(<1..531)
                     /locus_tag="PKZ53_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41994.1"
                     /translation="MSVQHHPFVQFLRQHDDTAWEQLLNRLLPDIHPVDKNATQIWFS
                     FFPYLLLREYENAPDKAVFTRDLLLQGKFLLADQVDSSHAFLFGYRYWAETKQIVVKL
                     VKETPRPTNLEALIRRVATSTAEYLHTEPSLVLGIAAVAFMTLQQVGWETFNHRADTA
                     IAPVPKSPKQLLADREA"
     gene            925..1815
                     /locus_tag="PKZ53_16020"
     CDS             925..1815
                     /locus_tag="PKZ53_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41995.1"
                     /translation="MLLLKRNYCDYEPVRATIDRARVIVVDSLIYSTTDLSDVAAQEP
                     DFNAEEWVKEERTIAGFAIGNIVQNVKKLVESPEVQVVSLAKISPQIVKDFDPDAIIL
                     SGTLRDFDMYQSSILKNVSTFLEATTVPVLGICGGHQLVGQAFGLPIVTLDHLEPHQR
                     RHNRLREYEYRFVKVIEPQDPIFKNVLTDHDIRRRSRGNTIWVWQNHGLMVDGMPDGF
                     QLLAKGYVCQNQMMVKRGDGQLIYTVQFHIEKSFEDWRKTPSRWEHPNESRDGRKLFE
                     NFLVEALKHRGQVFHHAQSA"
BASE COUNT          583 a          539 c          542 g          520 t
ORIGIN      
        1 agcttcacgg tcggcgagca gttgttttgg agacttgggc actggcgcaa tagcggtgtc
       61 tgcccggtgg ttaaatgttt cccatccgac ttgctgtagc gtcataaagg caaccgccgc
      121 aatccccaac accagcgatg gctcagtgtg cagatactca gcagttgacg tggcgactct
      181 tcgaatcaaa gcttcgagat tggtcggtct aggagtttct ttgacaagct tgaccacgat
      241 ttgtttggtc tcagcccaat accgataacc aaacaagaaa gcatgcgacg agtcgacctg
      301 atcggcgagc aggaattttc cctggagcag caagtcacgg gtaaaaactg ctttatccgg
      361 cgcattttca tactcacgca gcaacaggta cggaaaaaac gaaaaccaga tttgggtggc
      421 gttcttatca acaggatgaa tatcaggcag caaccgattg agaagctgtt cccaggcagt
      481 atcatcgtgc tgacgtaaaa attgcacaaa tggatgatgt tgaactgaca tagatgatga
      541 ctactttgaa ctatggagaa ttgagtgtgg agttgaaccg cgcatcatac tcattttttt
      601 taatttcgcc aatgaaaacc cgcgttcacc acaaatcgtg aattcgtctt ttgacaagcc
      661 cgtgtgagca aggtaaggta acaagacatt tggaatacct tgttcgtcac cgctcgacca
      721 atccaacaca atttgttcga aatttgctga gccctaactt ctcacgggcg tttgcggaca
      781 gaaccagaga attcattccc tccaggttgt ccctgatgcc ggctattccc cttctgtcag
      841 acccgttttg ccccttgact gacagaaata cttacggctt agtcaatcca tacgactgac
      901 gaacactgaa atgaggtttg gggtatgctt ttgctcaaga gaaattattg tgactatgaa
      961 cccgttcgtg ccacgattga tcgcgccaga gtcattgtgg ttgatagttt gatttattca
     1021 accactgacc tctctgatgt ggcggctcag gaacccgatt tcaatgctga agaatgggtc
     1081 aaggaagaac ggaccattgc cgggttcgcc atcgggaaca ttgtccagaa tgtgaaaaaa
     1141 ctggtcgaat cacctgaagt ccaggtcgtc agtctggcca aaatttcacc gcagattgtc
     1201 aaagattttg acccggatgc aattatcctc agtgggacgc tccgggactt tgacatgtac
     1261 cagtcgtcca ttttgaagaa cgtcagtacc ttcctggagg caacaaccgt ccccgttctg
     1321 ggaatttgtg gtggtcacca gcttgtcggc caggcatttg ggctaccgat tgtcaccctt
     1381 gatcacctgg aaccacacca gcggcggcac aatcggcttc gggaatatga ataccggttt
     1441 gtcaaagtga ttgaaccgca agaccccatt ttcaaaaatg tactcacaga ccacgatatc
     1501 cgccgacgca gtcgaggcaa tacgatttgg gtctggcaaa accacggatt gatggttgat
     1561 ggaatgcccg atggctttca gcttctggct aaaggatatg tgtgccagaa ccaaatgatg
     1621 gtcaaacgtg gggatggtca gttaatatat acagttcagt ttcatatcga aaaatccttt
     1681 gaggactggc gtaaaacccc gagtcgatgg gaacacccca acgaaagccg ggatggccgg
     1741 aaattgtttg aaaacttcct ggtcgaagcc ctcaaacacc gtggtcaggt gtttcatcat
     1801 gcgcaatcag cctgaaagac atcgggctcg gggctgaaaa atccttgggc tgaagactcg
     1861 caagctccgg gctgaaaaac ccttgggctg aagactcgca agctccgggc tgaaaagacc
     1921 agggctgaag actcgcaagc tcggggctga aaaacccttg ggctgaagtc tcgcaagctc
     1981 ggggctgaag aaaacgggct tggggcaatc gaagggatga ggggatgaag gatgagggat
     2041 gaaataaaac cagttcctca atccgagttc ttcagccctc agcccggttt cttcagccct
     2101 tagcccccag ccctatttct tcagcccgaa tgtcttcaaa ccctgaaccc taaaaccctg
     2161 aaccctaaac cctgaaccct aaaa
//
LOCUS       DAOQDO010001077         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47415_length_2183_cov_3.336466, whole genome shotgun sequence.
ACCESSION   DAOQDO010001077 DAOQDO010000000
VERSION     DAOQDO010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47415_length_2183_cov_3.336466"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PKZ53_16025"
     CDS             <1..1054
                     /locus_tag="PKZ53_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ41996.1"
                     /translation="ATPAVMALDLPAVGGEIRRFTGHSAVTSIAFAPNGRHFVSAKQD
                     KTIALLETETGKEISAFGNRRIFPYRCAVFSPDSCLVAVSGTDEAIRIYDLATGQEQN
                     RLAGHQQVTALAFSPDGKQLLSGSQDSSLCLWDLENRVQLHRMQGHIDNVANIVFASN
                     GSFALSIGLSGDLHLWNLEAGQEVQSLLSDAARPEAISVRVSIDGRRVLGGDSHPGHS
                     GGETKQRMRRLEGKATPIGSGVAISPDRTKAISSSQDTTLRLWDLVEGREAARFLGHG
                     NYVTSVAISADGTQALSGSEDRTIRLWDLASGQELACCMGHKDKVYKVAFLPGDRHAI
                     SCSYDGTVRLWGLWKD"
     gene            1369..>2183
                     /locus_tag="PKZ53_16030"
     CDS             1369..>2183
                     /locus_tag="PKZ53_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ41997.1"
                     /translation="MKLRRLVFFSCLLIAMLGLQVAPVALLAKSKPKAAAPRQGAPDF
                     SRLVVIGASISAGFQSNAFFQSGQVASYPNLLAKQVGSQIVLPLISDPGIPPNGQLQL
                     TKPVCSAKVGDSAAIQGFGTRANPTQQATLLSVPGHKLLDVLSTRPTTLDPSKITITD
                     FVLGLPGLLAPTPIAMSQIEWVAALKPTTLLVAEVGGNDALGAVLTGTDASLTPLEDF
                     RTRYTEIMTKAKASGASTIVTLNIPDLTSFAVVFSQDDISRNFGINKETLQFV"
BASE COUNT          495 a          599 c          558 g          531 t
ORIGIN      
        1 agcaacaccg gctgtcatgg cacttgattt gccggcggtg gggggggaaa tccgacgctt
       61 taccggccat tcagcggtta ccagcattgc ctttgctcca aatggtcgcc actttgtttc
      121 agcgaaacag gataaaacca tcgccttgct cgaaacggaa actggaaaag aaatcagcgc
      181 ttttggcaac cggcggattt ttccctaccg ttgcgccgtg ttttcaccgg atagttgttt
      241 ggtggcagtg agcggcacag atgaggcaat ccgaatttat gacctggcga ctggccagga
      301 gcaaaatcgg ctcgctggcc atcagcaggt taccgccctg gccttttcac ccgatggaaa
      361 gcaattattg tcaggaagtc aggacagcag tttatgcctg tgggatctgg agaaccgggt
      421 ccaactccac cgcatgcagg gtcatattga taatgttgcc aatatcgtct ttgcttcaaa
      481 cggatcgttt gcgctctcaa ttggcctgag cggtgacctg cacttatgga atctggaagc
      541 tggacaggaa gttcaatccc tcctctcaga tgccgcccgg ccagaagcaa tcagcgttcg
      601 ggtctcaatt gatggacggc gggtccttgg aggtgactct caccccggtc attccggagg
      661 tgaaaccaag cagcgcatgc gacgcctgga aggcaaagcc accccaatcg ggagcggcgt
      721 ggccatttcc ccagaccgaa ccaaagcaat ttcgagcagt caggatacga cgttgcgcct
      781 gtgggatctg gtcgaaggcc gtgaagccgc ccgctttttg ggccatggca actatgtcac
      841 cagcgtcgcg atttcggctg atggcacgca ggcgctttcc ggcagtgaag atcgcaccat
      901 ccgcttgtgg gatctggctt caggtcagga actggcctgc tgtatgggcc acaaagacaa
      961 agtctataaa gtcgccttcc tgcctggcga ccgccatgcc atttcctgca gctatgatgg
     1021 gacggtaaga ctctggggac tgtggaagga ttgagaattg agaatgaaga attgagaatg
     1081 aagaattgag aatgaagaat gattttttgt tctgccattc tgccattctg ccacagcatc
     1141 attctgccaa attccacttg cccttaacct gcgtcactcc taagattaag aactcttcgg
     1201 catcgtctaa aatcaagaat gaagtatgag gagtttcctt tgtgagacca cacagagttt
     1261 gtgatcttct ctttcttaac tcttcattct tcattcttca ttcttctgac tccagctcat
     1321 cctaacgcta acgttcctaa tccactgttc aactctcgag ggggaaacat gaaacttcgt
     1381 cgactggtat tcttttcttg cctgctgatt gccatgcttg ggcttcaggt ggcaccagtt
     1441 gctctactgg ccaaatcaaa acccaaggca gcagcaccac gccagggagc accggacttc
     1501 tctcggctgg tggtcatcgg cgccagcatc tcggctggat tccaaagcaa tgcgtttttc
     1561 caatccgggc aggtcgcgtc ctatccaaat ttgctggcca agcaggttgg atcacaaatc
     1621 gttctgccat tgatttcgga tccaggaatt ccacccaacg gccagctcca actcaccaaa
     1681 ccagtctgtt cggccaaggt gggtgattca gccgccattc agggtttcgg gacacgagcg
     1741 aacccgacgc aacaggcaac cttgctgtct gtccccgggc acaaactgct cgatgtgctg
     1801 tcaactcgcc caaccacgct tgatccttca aaaatcacca tcaccgattt tgtgcttgga
     1861 ttaccaggtc tgctggcccc aaccccgatt gccatgagcc agatcgaatg ggtggcggct
     1921 ctcaaaccga caaccctgct ggttgccgaa gttggtggca atgatgccct cggtgcggtt
     1981 ctgaccggaa ccgatgcgtc attgaccccg ctggaagatt tccgtacccg atacactgaa
     2041 atcatgacca aggccaaggc gtctggggcg tccacgattg tgacgctcaa tattcctgac
     2101 ctgacctcat ttgcggttgt tttttcccag gatgatattt cccgcaattt cgggatcaac
     2161 aaggaaacgc tccagtttgt gtt
//
LOCUS       DAOQDO010001078         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47418_length_2183_cov_3.155545, whole genome shotgun sequence.
ACCESSION   DAOQDO010001078 DAOQDO010000000
VERSION     DAOQDO010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47418_length_2183_cov_3.155545"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKZ53_16035"
     CDS             <1..431
                     /locus_tag="PKZ53_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ41998.1"
                     /translation="SYDEVNKNTGEYLIKGVSARVLQQMLWKLIPNYELQNVGSYYNL
                     WKEAEGFDVSGTRVPKSMYILNAIDHYNQDYSGGAQTIINQIYFPAVHNFQMFQKAFL
                     AEGRKYWKYYNTKTFKLAFDQDQEWLSGLVPLLKRYYYGL"
     gene            449..>2183
                     /locus_tag="PKZ53_16040"
     CDS             449..>2183
                     /locus_tag="PKZ53_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNJ41999.1"
                     /translation="MQTFSEKSTTPASSPRSVFADPNAVTVQGMELKEELSPMDPLQA
                     KLNQTSSVAIQMKLQDSFSQNPRLVAQAKLGAKLAQRKALIQAKCDMCEVSESEEKLK
                     KLKKESDPGEDEDDAVIQSKAAGSLTSSDTPVPATNNTGLPDDLKTGMESLSGMSLND
                     VNVHYNSAKPAQLQALAYTQGTDIHVGPGQEQHLAHEAWHVVQQKQGRVQPTTQLQNV
                     AINDDFGLEREADVMGGKALQAKADGHFENLQELNQGVISFKTPVQLLEMETDDGTWD
                     FSDYAIQNSGGWRGLAEHTLTYRPNGGIGEDRIALIQTVRSEIDGEIVHSNATHQNRT
                     SEQTGFRIDNFTDSNNPIFGAASATSLANTALQDDTFQVGGRVNRGTSRRPNIVDQEP
                     NRLDNPGFGIRDLQRQNLSNVAMGQTFITAAYNMTTGQYLGSIAYGWEQAEDGEPQLL
                     EMTYTTGGVSDTFLSAVTKWNQGRTRGRNNIQLPTPQGVEVQGFEEEEDDLIEDLDQM
                     EDNPVELDDVDMGGQELSFEQWVQQLQHWFFQEYQASLFDYPDEPYRLEYEDGMSPQD
                     FYAQYLANGRAF"
BASE COUNT          651 a          512 c          529 g          491 t
ORIGIN      
        1 tcagttatga cgaagtcaac aaaaatactg gcgaatacct gattaaaggt gtgtctgcca
       61 gggttttaca gcagatgctt tggaaattga ttccaaatta tgaactccaa aatgttggtt
      121 cctattacaa tttatggaaa gaggcagaag gttttgatgt cagtggaacg agagtcccga
      181 aatcaatgta catcctgaat gcgattgatc attacaatca ggactacagt ggcggggctc
      241 agaccatcat aaaccagatc tatttcccag cagttcataa ttttcaaatg tttcaaaaag
      301 cattcctggc tgagggtcga aaatactgga aatattacaa caccaaaact tttaaacttg
      361 ccttcgatca ggatcaagag tggctgtcag gactggttcc gctcctgaaa cggtattact
      421 acggcctgtg aaaaattgag aggactttat gcagactttt tccgagaaaa gcacaacccc
      481 agccagttcc ccccgctctg tttttgccga cccaaatgct gtgacagttc agggaatgga
      541 attaaaagaa gaattgtcac caatggatcc ccttcaggcg aaattgaacc agacttccag
      601 cgtggcgatt caaatgaagc tccaggatag tttcagtcag aacccccggc tggtagccca
      661 ggccaaactc ggagcaaagc tggcgcaacg gaaagctcta attcaagcca aatgtgatat
      721 gtgcgaagtt tcggagtcag aagaaaaact caagaaactc aaaaaagaat ctgacccagg
      781 cgaagatgaa gacgacgcgg ttatccagtc aaaagcggca gggagtttga cctcatcgga
      841 cacgccagtt ccagcgacaa acaacactgg tcttcctgac gacctcaaaa ctggcatgga
      901 atcgctttcc ggtatgtcgc tcaatgatgt caacgtgcat tacaattcag ccaaaccagc
      961 ccagttgcag gcgctggctt acacccaggg aacggacatc catgtcggcc ctgggcagga
     1021 acaacacctc gcccacgaag cgtggcacgt cgtccagcaa aaacagggcc gggttcagcc
     1081 aacaacgcag cttcaaaatg tcgccatcaa tgatgatttt ggactcgaac gcgaagcgga
     1141 tgtcatggga gggaaagccc tgcaggcaaa ggcagatggt cattttgaaa atcttcagga
     1201 gttaaatcag ggagtcatca gtttcaagac accagtccaa ttgctggaaa tggaaaccga
     1261 tgacggaacc tgggatttta gcgactatgc cattcaaaat tcaggtggat ggcgtggact
     1321 tgccgaacat accttgacct atcgtccgaa tggcggaatt ggagaagatc gaattgcctt
     1381 gattcaaacc gttcgatctg agattgatgg cgaaattgtc cattcgaatg caactcatca
     1441 aaaccgaacc agtgaacaaa caggatttcg aatagataat ttcactgatt caaacaatcc
     1501 aatttttggt gccgcgtcag ccacatcact ggcaaatacg gcactgcagg acgatacctt
     1561 tcaagttggc ggacgggtca atcgtggcac cagtcgccgt ccaaacattg tagatcagga
     1621 gccaaatcgg cttgataacc caggttttgg gattcgtgac ctccaaaggc aaaatctatc
     1681 aaatgtcgct atgggccaaa catttatcac tgcggcctac aatatgacga ctggtcaata
     1741 tctcggctcc atcgcttatg gttgggagca ggcagaagac ggagaacccc aattgttaga
     1801 gatgacttac accactggcg gcgtttcaga taccttcttg tcagcagtaa ccaaatggaa
     1861 tcaagggcga actcgtggcc gaaataatat tcaactgcca actccgcaag gggttgaagt
     1921 tcagggattc gaggaggaag aagacgatct catcgaggat ctcgaccaaa tggaagacaa
     1981 cccggtggaa ctggacgatg tggacatggg tggccaggaa ctgagctttg agcaatgggt
     2041 tcaacaactt cagcactggt tcttccagga ataccaggcc agcctgtttg attacccgga
     2101 tgaaccctat cggcttgaat atgaagatgg aatgtctccc caggacttct atgctcagta
     2161 cctggcaaac gggcgagcat tct
//
LOCUS       DAOQDO010001079         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47461_length_2182_cov_3.272214, whole genome shotgun sequence.
ACCESSION   DAOQDO010001079 DAOQDO010000000
VERSION     DAOQDO010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47461_length_2182_cov_3.272214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..>2182)
                     /locus_tag="PKZ53_16045"
     CDS             complement(82..>2182)
                     /locus_tag="PKZ53_16045"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ42000.1"
                     /translation="VQTHDGYLWFGTTDGLVRFDGVKFKVFNKANSEGLGSNRISQTL
                     EDRSGRLWVTTEDGRLMYYQHGAFTVVLHPNDPQYWPRRNLVDDGHGSILFYKIESRQ
                     TFQFRDGKLVLFEPSGMIPGTEILYADRSGGYWALDGLDFHRVKDGRRQTFSTLPSGK
                     TKSVYEDRFGHYWLTTGLKDIYRIADNRIERFESPVTGLWGCLEDKAGNLWFKTFNEG
                     VVRIEAAEVHSGELTPEKFRQFTDQDGMLNNIGRNLAVDREGGVWVGTEKGLHHFYLQ
                     TVEVYSKKDGLESDNVYPILEDRQGAIWFGGWPFSLMKFENGRFSTVVGSTQFPHYSA
                     LCESRDGTIWAANDENVFYLQNETPVIFTPPIESGVVLKGVNVILQSLDGAMWFGSGN
                     GLVRYLAGKFTTFTRVDGLPDTFVVALIQARDGRLWVGTRGGIACLENGKFRAFTEKD
                     GLASNYTRCLYEDTDGILWIGSYDGGLTRYRDGRLTRFTMFNGLHSNGVFCILEDNRG
                     WFWMNSNQGIYRVRKQELNDFTEGKTKFLTSIAYGKKDGLINIEGNGGRQPAGFRGRD
                     GKLWFPMAQGVAMIDPEKVIINPLPPPVLIEEISIDRTPIPNDTFQAAIKSLQTPVRI
                     DPYQNNLEIQYTGLSFIDSTQVKFKYKLEGWDPDWIEAGMRRTAYYSYLPAGEYTVIG
                     ASGGAPVYVLLRLSFPD"
BASE COUNT          540 a          616 c          506 g          520 t
ORIGIN      
        1 gtccgctctc ccgccgaaaa gaaaaatccc cgccgacgcc gaagcgtccg cggggatttc
       61 tttgcgatgc gtgagagacg atcagtcggg gaacgagagg cgcagcagca cgtagaccgg
      121 cgctccgccc gatgcgccga tcacggtgta ttcgccggcg ggcaaatagg aataataggc
      181 ggtgcgacgc atcccggcct caatccagtc tggatcccac ccttcaagtt tgtatttgaa
      241 tttgacctga gttgaatcaa taaaactcaa gccggtatat tggatttcca ggttgttttg
      301 atatggatca atccgaacag gggtttgcaa acttttgata gcggcttgaa acgtgtcatt
      361 cggaatcgga gtgcgatcaa tcgagatttc ttcaatcaac actggtggcg gcaagggatt
      421 gatgatgact ttttctggat caatcattgc cacgccctga gccatgggga accagagctt
      481 tccgtcacgg ccccggaatc cggctggctg gcgtccgccg ttgccttcga tattgatcag
      541 gccatccttt tttccatagg caattgaggt caggaacttg gtctttcctt ccgtaaagtc
      601 attgagttcc tgttttcgaa cccgatagat gccctgattg gagttcatcc aaaaccagcc
      661 gcgattgtct tctaaaatgc aaaacacacc gttgctatgc aacccattga acatggtgaa
      721 tcgcgtcaac cgaccgtccc gatagcgggt caatccgccg tcataactcc cgatccagag
      781 aatgccatcg gtatcttcat aaagacagcg ggtataattg cttgcgagcc cgtctttttc
      841 ggtaaaagca cgaaatttcc cgttttccag gcaggcaatc cccccacggg tgccaaccca
      901 gagccggcca tctctggcct ggatcagtgc caccacaaag gtatctggca gaccatctac
      961 tcgtgtaaac gtggtgaatt tcccagccag ataccgcacc agcccgttac ccgagccaaa
     1021 ccacatggcg ccatcgaggc tttgtaaaat gacattgacc cctttgagca ccactccaga
     1081 ttcgataggc ggggtgaaaa tgacgggggt ttcgttttgc aaataaaaga cattctcatc
     1141 gttggccgcc cagatggttc cgtctcgtga ttcacacaga gctgagtaat gagggaattg
     1201 agtgctgccc accacggttg aaaatctgcc gttctcaaac ttcatcaagc taaacggcca
     1261 gcccccaaac cagatcgcgc cctgtcggtc ttccaaaatt gggtagacat tatcactctc
     1321 taacccatct ttttttgaat aaacctcgac agtttgaaga taaaaatgat gcagcccttt
     1381 ttcggtgccg acccagacac caccttcacg atcaacggcc aggttgcgac caatattgtt
     1441 gagcattcca tcctggtctg taaattgacg gaatttctca ggggtcaatt cgcccgaatg
     1501 cacttcagcc gcttcaatcc gcacaacccc ttcattaaaa gttttaaacc acagattccc
     1561 ggctttatcc tcaagacatc cccagagacc agtcaccgga gattcaaacc gttcgattcg
     1621 attgtcggca atccggtaaa tatctttcaa cccggtggtc aaccagtagt gcccaaaccg
     1681 gtcttcatac acggatttgg tttttcccga agggagggtt gagaaagtct gtcgccggcc
     1741 atccttcacc cgatggaaat caagcccatc cagcgcccaa tatccacctg agcgatcagc
     1801 atacagaatt tcagtcccag gaatcattcc cgacggctca aacagaacca gcttcccatc
     1861 ccggaattgg aaggtttgtc gcgattcaat cttgtaaaaa aggatgctgc cgtgcccatc
     1921 atccaccagg ttccggcgag gccagtattg gggatcgttt ggatgtaaga ccacagtgaa
     1981 ggcaccatgc tggtaataca tcagccgacc atcttcagtg gtgacccaca atcggccact
     2041 ccggtcctca agtgtctggc tgatgcgatt gctgccaagc ccctcgctat ttgccttgtt
     2101 aaacactttg aacttcacgc cgtcaaaccg caccaggcca tcggtggttc caaaccacag
     2161 atatccatca tgggtttgca cc
//
LOCUS       DAOQDO010001080         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47493_length_2181_cov_3.457197, whole genome shotgun sequence.
ACCESSION   DAOQDO010001080 DAOQDO010000000
VERSION     DAOQDO010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47493_length_2181_cov_3.457197"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKZ53_16050"
     CDS             <1..484
                     /locus_tag="PKZ53_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42001.1"
                     /translation="EGLFMAAKGHPEDPGTMKAVKLRLACQSPDTDRIQKTLQLVSQT
                     GIAPGEDLVYTKLCLAFHPELAEELGPRQALFWILPHTGEETEAKIGDQIYIGHLQLT
                     LESIRSLDDGYPLKAGICLKYSRPASGITPKRWIPVRLQAALRLRGARHHVLHTFTET
                     "
     gene            598..1524
                     /locus_tag="PKZ53_16055"
     CDS             598..1524
                     /locus_tag="PKZ53_16055"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNJ42002.1"
                     /translation="MAPETTEKIWHNGVFINWEDARIHVMSHVIHYGSSLFEGIRCYS
                     TPKGPQIFRLHEHIRRLFDSCRIYRMDIPHSLQELSQVCTEIVRVNHMEECYVRPIVF
                     RGYGTFGVNPFPAPVETYIACWPWGPYLGSDALEEGVDVCTSTWSRMSPNTLPTTAKA
                     GANYMNSQLVKMEAIVNKYAEGIALDRFGYVSEGSGENIFLVRDGVVHTPPSHASVLP
                     GITRDAVMTLCHELGIPLKERNISREELYIADEIFLTGTACEITPIRSIDRINVNNGQ
                     RGNITARLQERFFGIFNGEYEDRYHWLTLVNK"
     gene            1524..>2181
                     /locus_tag="PKZ53_16060"
     CDS             1524..>2181
                     /locus_tag="PKZ53_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNJ42003.1"
                     /translation="MDSFSALTSEIENCRQCPRLVQWREQVAAEKVARFRHQTYWGKP
                     VPGFGDPQARLVIVGLAPAAHGGNRTGRVFTGDKSGDWLYRALHKAGFANQPDSTDAQ
                     DGLQLTDCYIGVVVRCAPPENKPTPPETETCRAFLDRELKLLESTQVILTLGKFAFDN
                     VLKILKSQGQAIPSPAPQFGHNRGYHLGTKDPPLFLIGSYHPSQRNTSTGFLTEPMFD
                     E"
BASE COUNT          539 a          574 c          557 g          511 t
ORIGIN      
        1 ggaagggtta tttatggcgg ctaaagggca tccggaagat cccggtacta tgaaagcggt
       61 caaattacga cttgcctgcc agtcaccaga cactgatcgg attcaaaaaa cactccaact
      121 ggtgtcccaa accggcattg cgccaggtga agacctggta tataccaagt tatgcctggc
      181 gtttcatcca gaactggctg aagagttagg cccccgccag gcactgtttt ggatccttcc
      241 acacaccggc gaggaaaccg aagccaaaat tggtgatcaa atttatattg gccatttaca
      301 attaactctg gagtcaattc gcagtcttga cgatggatat ccactcaaag ccggcatttg
      361 cttgaaatac agccgcccgg catcgggaat tacccccaag cgctggatac cagttcggct
      421 ccaggccgct ttacggttgc gtggtgcccg ccaccacgtg ttgcacacct tcactgaaac
      481 ctgattggtc cggggtgttt gatccctgta tcagacaccc aaactcaacc accagccgtc
      541 aaccacgcga gatcagatgt gctttaccaa acttaacgag aagaaatcga gggaattatg
      601 gcaccggaaa caactgagaa aatctggcac aatggagttt ttattaattg ggaagatgcc
      661 cgcatccatg tcatgtcaca cgtgatccat tacggctctt cattgtttga agggatccgg
      721 tgttacagta cacccaaagg gcctcaaatt ttccgactcc acgagcacat ccggcggttg
      781 tttgattcat gtcgaatcta ccgaatggat attcctcatt cacttcagga attgagtcag
      841 gtgtgtactg agattgtgcg agtcaaccac atggaagaat gctacgtgcg tccgattgtg
      901 ttccgtgggt atggtacttt tggggtcaat ccatttccgg cgccggttga aacctacatt
      961 gcctgctggc cctggggacc ctatctgggg tcagatgcac tcgaagaagg ggttgacgtc
     1021 tgcacctcga cctggtcgcg gatgtcaccc aacaccttgc cgacaaccgc caaagctggc
     1081 gccaattaca tgaattcaca actggtcaag atggaggcca tcgtcaataa atatgccgaa
     1141 gggattgccc ttgaccgctt tggctatgtc agtgaaggca gcggagagaa tattttcctg
     1201 gtgcgggatg gggtggttca cacaccgccg tcgcatgctt cagttttacc agggattacc
     1261 cgtgatgcgg tgatgacctt atgccacgaa ctgggaatcc cgctcaagga acgcaatatt
     1321 tcgcgcgaag aactctatat cgctgatgaa attttcctga cgggaacggc ctgtgaaatt
     1381 actccgatcc gaagcattga ccgcatcaat gtcaataatg gccagcgggg caatatcacc
     1441 gccagattac aggaacggtt tttcggtatt tttaacggag aatatgaaga ccggtatcac
     1501 tggctgaccc tggtcaacaa atgatggatt cgttttcagc cctgacatct gaaattgaaa
     1561 actgccgcca gtgcccacgg ctggttcaat ggcgagagca ggttgctgct gaaaaggtgg
     1621 cccgcttccg ccatcagacc tattggggaa aacccgtgcc ggggtttggt gatccgcaag
     1681 cccggcttgt catcgttggg ctggcaccgg cggctcatgg tggcaaccga accgggcggg
     1741 tttttacagg cgacaaaagt ggagactggt tataccgggc acttcacaaa gctggctttg
     1801 ccaatcagcc tgattcaacc gatgcccagg atggcttgca gctcaccgat tgttatatcg
     1861 gggttgtggt gcgatgtgct ccacctgaga acaagcccac cccacctgaa accgaaacct
     1921 gccgggcctt ccttgaccgc gagttgaaac tgcttgaatc aacccaggtg atcctgacgc
     1981 ttgggaagtt tgcctttgac aatgtattga aaatcttgaa atcccagggg caggccatcc
     2041 cctcacccgc cccccaattt ggacacaatc gagggtatca tctgggaaca aaagacccgc
     2101 ccctgttcct gattgggtcc tatcacccaa gtcaacgcaa tacgtcaacc ggatttttga
     2161 ccgaacccat gtttgatgag g
//
LOCUS       DAOQDO010001081         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47532_length_2180_cov_3.266353, whole genome shotgun sequence.
ACCESSION   DAOQDO010001081 DAOQDO010000000
VERSION     DAOQDO010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47532_length_2180_cov_3.266353"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKZ53_16065"
     CDS             complement(<1..423)
                     /locus_tag="PKZ53_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42004.1"
                     /translation="MKAGKLYRSLGMLAIVLMLGTISVLAAPTGGSKFNHTTQSKEAK
                     QYFDQAIQALESYALPPQINGLAKKAIDLDPDYALATYIYGVTLTPPQDPAPVVQKAF
                     ELGKKASDGERRYLEAVQLIRQQKATEALPILKELSTQF"
     gene            complement(606..1037)
                     /locus_tag="PKZ53_16070"
     CDS             complement(606..1037)
                     /locus_tag="PKZ53_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ42005.1"
                     /translation="MSKTNVAELPVNGTVVEKEAPSEPLIPVTNTDSTSYSMLEPRMK
                     KVYGAYYKELYFTPERRVLSPKVQELISIAASLVAKCEGCIDGHMKKALELGATKEEI
                     SETICIAAAINAASIIDLSDGTAARLGLNHFPSKPRPKQEE"
     gene            complement(1107..1703)
                     /locus_tag="PKZ53_16075"
     CDS             complement(1107..1703)
                     /locus_tag="PKZ53_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42006.1"
                     /translation="MKSAHTSLVRSAIFALIITICTSCTAFAFAKQDKRKATKIQSAT
                     VAVGKNKAAVEVRYLNLPWGETTFGYIENGGNQYYSNRTWPFAHLKLGVKAKFNGSEI
                     TPGDYVMYITPKSGDTPMTLSIASFKPNSTGTFLQDGNVFTETPKDVVEVAKKAVTFE
                     KGDTLINELKIDLASDKSDVAFKVHYGNRLMTEKLNLK"
     gene            complement(1848..>2180)
                     /locus_tag="PKZ53_16080"
     CDS             complement(1848..>2180)
                     /locus_tag="PKZ53_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42007.1"
                     /translation="VYHHVSMMNLPTFPFAPILLWVTAWRLGFTPDDYNVQTAVRHIS
                     CPILFIGSGADTRMPNDTVLEPLFAAARNPLKQKFIVAGARHGHAYDVAPLEYQKIVA
                     QFLRSISM"
BASE COUNT          495 a          470 c          608 g          607 t
ORIGIN      
        1 aaattgggtt gaaagttctt tgaggattgg cagggcttcg gtggcttttt gttgccgaat
       61 gagttggacg gcttccagat atcggcgttc accatccgag gcttttttgc ccagttcaaa
      121 tgctttctgc acgaccgggg ctgggtcctg tggcggggtc aaggtcacac cgtaaatata
      181 agtcgccaga gcatagtccg gatccaggtc aatggctttc ttggccaatc cgttgatctg
      241 cggcggcagc gcatagctct ctagcgcctg aattgcctgg tcaaaatact gtttggcttc
      301 tttggattga gtcgtgtggt tgaatttact gccgcctgtg ggggctgcca aaacggaaat
      361 agttccgagc atgagcacaa tggcgagcat gccgagggaa cgatacagct ttcctgcttt
      421 catgggtggg gtcctcttct ctgaataggt agtgtggtct ggtgaccaca tcaatatgtg
      481 cacgatgagt tttggcctgt tcaagcgtac accacgacct tgatcaggca gtcaattgac
      541 tgcctgacag gcaacgtcgt gtatggaaaa tcggtagggt acgcagtggt cgagtggcgg
      601 agtaattact cttcctgttt ggggcgtggt ttgctgggaa agtgatttaa tcccagccgt
      661 gccgctgtac catcgctgag gtcaatgatg gaggccgcat tgatggcggc ggcaatgcag
      721 atggtttcgc tgatttcttc tttagtggcg ccaagttcga gggccttttt catatggccg
      781 tcaatacaac cttcgcactt ggcgaccagt gaggccgcaa tggaaatcaa ttcctgaact
      841 tttggggaca gtacccggcg ttccggggtg aaatacagct ccttataata cgcgccgtat
      901 acttttttca tccgtggctc aagcattgag taggaggttg agtcagtatt ggtgactggg
      961 atcaatggtt ctgacggggc ttctttttca accacggtgc cattcacggg taattctgcg
     1021 acgttggttt tactcataac aaaaaagtct cctgaaattc gagcggaaag ggcggcctgg
     1081 gatatcagcg tgccggaaat caagtgctat ttaaggttca gtttttcggt catcaaccgg
     1141 ttgccatagt gcactttgaa cgcgacatcg cttttgtcgg atgccagatc aatcttcaac
     1201 tcattgatca aagtatcgcc tttttcaaag gtcactgctt ttttggcaac ttcaaccaca
     1261 tctttggggg tttcagtgaa gacattgcca tcctggagga aggtgccagt gctattgggt
     1321 ttgaaggagg caatcgagag cgtcattggg gtgtcgccgc tttttggtgt gatgtacatc
     1381 acataatcgc caggggtaat ttctgagccg ttaaatttgg ctttgacgcc aagtttcaaa
     1441 tgggcaaatg gccaggtccg gttgctgtag tattgatttc cgccgttttc gatataccca
     1501 aaggttgttt caccccaggg caggttcagg tagcgaactt caacagccgc tttgtttttc
     1561 ccgacagcaa cggtcgcgct ttgaattttg gttgctttcc gtttgtcctg tttggcgaaa
     1621 gcgaaggcgg tacatgaggt gcaaatggtg atgatcaagg caaaaattgc cgaacgcacg
     1681 agactggtgt gtgcagactt cattgggaga aatcctttct gattttggtg aagagatatg
     1741 tctttgattg atttgatgtg tggatatcgc gcggtacgtg gaaatgcaat acccggttgc
     1801 gcctgaaagg gctttttaca aaatgcttac atgttgggct aagtttttta catactgata
     1861 cttctgagaa attgagccac aattttctga tactccagag gggccacatc ataggcatga
     1921 ccatggcggg cgcccgccac gataaacttt tgtttgagtg ggttgcgggc agcagcaaaa
     1981 agtggttcta agacggtatc gttgggcatg cgggtatcgg caccactccc aataaataaa
     2041 attgggcacg aaatatggcg taccgccgtc tggacattat agtcatctgg ggtaaaaccc
     2101 aatcgccagg ccgtgaccca caacaaaata ggagcaaatg ggaacgtcgg caggttcatc
     2161 attgagacgt ggtggtatac
//
LOCUS       DAOQDO010001082         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47542_length_2180_cov_2.998588, whole genome shotgun sequence.
ACCESSION   DAOQDO010001082 DAOQDO010000000
VERSION     DAOQDO010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47542_length_2180_cov_2.998588"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKZ53_16085"
     CDS             complement(<1..589)
                     /locus_tag="PKZ53_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNJ42008.1"
                     /translation="MKPTSSHHLVGILWTLIACFLFGSAGTVVRLGVAAGAHTLTVMV
                     VQTLFATAIAWTIAGQIPRLTRDIAWRMFLLALAAGTSGWCMLEGYARLGVAPVAIIL
                     ALYPAIVGLYVWILDKKRPAGMWFVSLGTAFVGVLLAMIGQQQAAGHWWEYLFPIVCA
                     FTLAANDLLVQRVVVNTPPSIAIGWINLFTAITTFV"
     gene            complement(759..1250)
                     /locus_tag="PKZ53_16090"
     CDS             complement(759..1250)
                     /locus_tag="PKZ53_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42009.1"
                     /translation="MSSKPSKPLVTERSCPKCEARITFPDDVKTVLCPKCRQSLRVFR
                     EDGKLHLREPDRKDDLAQVQSELDQIDLEMTVLLEKVTQMERSSFALAGFSIVPFGVV
                     LYALKEALHPKGDWTFFAIALAVFLGCLGGAYWVAFNPPANRKIMKLMEDRITLRRRM
                     EAM"
     gene            complement(1352..>2180)
                     /locus_tag="PKZ53_16095"
     CDS             complement(1352..>2180)
                     /locus_tag="PKZ53_16095"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNJ42010.1"
                     /translation="KLESNVAEANQVLAARSASKAEPVAVSPELIDQTCEMAPLVTDT
                     AMYLTKAILAGKTVLFEGAQGTMLDIDHGTYPFVTSSNGTSGGATVGTGVPPTMITGV
                     LGIMKAYTTRVGGGPFPTELHDSVGEHIRERGHEYGASTGRPRRCGWFDSVVSRYAVL
                     INGLSSIAMTKLDVLDTLPELKICIGYRHNGTVLTDIPHDATILEAVEPVYETFPGWQ
                     ASTAGITTYADLPEKARAYVDRLTELVGCEIGLISTGPDRNETIIREDSALASWLKD"
BASE COUNT          558 a          592 c          550 g          480 t
ORIGIN      
        1 gtacaaatgt cgtgatggca gtaaagaggt tgatccagcc aatagcgata ctgggtggcg
       61 tattcacaac cacccgttga accagcaggt cattggcggc cagcgtaaag gcgcaaacga
      121 ttggaaataa atattcccac cagtgccctg ctgcttgctg ctggccaatc atcgctaaaa
      181 gcacaccaac aaaagcggtt ccgaggctga caaaccacat tccggctggt cgctttttgt
      241 ccaaaatcca gacatacaat ccaacaatcg ccggatataa ggccagtata atcgccaccg
      301 gcgcaacacc gagccgggca tagccttcga gcatacacca gccgctggtt ccagcagcca
      361 acgctaaaag aaacattctc caggctatat cacgggtcaa tcgcgggatt tggccagcaa
      421 tggtccaggc aatcgcagtg gcaaacagcg tttgaaccac catcaccgtc agcgtgtgcg
      481 caccggccgc aaccccaagt cgaacaactg ttcccgctga accaaataag aaacaggcga
      541 tcaacgtcca caaaattcca accagatgat gcgaagatgt aggtttcaat gattgaggct
      601 cagagggtgt tggttgaatg gtcaagggat gagaatgtca gtaccgcctg cgtgagcggg
      661 tggcggttga gaatgtcagg gtcacctggc tgagcagatg gatatcattt gtcatcagac
      721 ccacccgctc acgcaggcgg tactgacaat ttgccgggtt acatcgcttc cattcgccgg
      781 cgaagggtaa tgcgatcttc catcagcttc ataatcttgc ggttcgctgg cgggttaaat
      841 gccacccaat aggcgccacc aaggcatccc agaaagactg ccagtgcaat tgcaaaaaat
      901 gtccagtcgc cttttgggtg gagggcttct ttcagggcat agagcacaac cccaaatgga
      961 acgattgaaa acccagccag ggcaaaactg gaccgttcca tctgggttac tttttcaagc
     1021 aataccgtca tttccaaatc aatctggtca agctcgctct ggacctgagc caaatcatcc
     1081 ttgcgatctg gttcacgcag gtgaagtttg ccatcttcgc gaaaaactcg aagtgattgg
     1141 cgacacttgg gacataaaac cgttttgacg tcatccggaa acgtgatgcg ggcttcacat
     1201 tttggacaac tgcgttcagt taccagcggt ttggaaggtt tgctgctcat atccacgtcc
     1261 ctctcgaaaa ttgggtgact ggtgactggt gtttagggct ttgaaaatat tgattccgaa
     1321 gtcccccaac cagttaccag tcaccaaact cctaatcttt gagccacgaa gccagcgccg
     1381 aatcttcccg aatgatggtt tcgttgcggt caggtccagt cgaaatcaaa ccaatttcgc
     1441 acccaaccag ctcagtcaac cggtcaacat aagcccgcgc tttttccggc agatccgcat
     1501 aggtggtaat gcccgccgtt gaagcctgcc agccagggaa ggtttcatat actggctcaa
     1561 ccgcttccag aatggtggca tcgtgcggaa tatcggtcaa aaccgtgccg ttatgacgat
     1621 agccaataca gattttgagt tctggcagcg tatcgagcac atccagcttg gtcatggcga
     1681 tagatgaaag tccattgatc agaacggcat agcgcgatac aaccgagtca aaccagccac
     1741 atcggcgtgg acggccagtc gaggcgccgt attcgtggcc gcgctcccgg atgtgttcac
     1801 caacgctgtc atggagttca gttggaaatg gcccgccacc aacacgagtg gtataggctt
     1861 tcataatgcc caaaaccccg gtgatcatgg tcggaggaac acccgttccc acggtggcac
     1921 cgcccgaagt tccatttgaa gacgtcacaa atggataggt tccgtggtca atatccagca
     1981 tcgttccctg ggcgccttca aacaggacgg ttttcccagc cagaattgcc tttgtcaaat
     2041 acatggccgt atcggtcacc aacggggcca tttcacaggt ctggtcaatc aattctggac
     2101 tgacagcaac tggttccgct ttagaagcac ttcgggcggc cagcacctga ttggcttcgg
     2161 caacgttgga ttcgagcttg
//
LOCUS       DAOQDO010001083         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47585_length_2179_cov_3.076271, whole genome shotgun sequence.
ACCESSION   DAOQDO010001083 DAOQDO010000000
VERSION     DAOQDO010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47585_length_2179_cov_3.076271"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="PKZ53_16100"
     CDS             complement(<1..1298)
                     /locus_tag="PKZ53_16100"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ42011.1"
                     /translation="MDELQTELRKPYLIVTGRSMNPSKIEIPGSVFSIGRGTDNDLQI
                     SDTLISRMHAEIILVAEGQYLLRDKGSKVGTIIKGERINEYLLEPGDEIILGDPSIAK
                     LKFEYPAYVSTSSLNPIDTSRIDLRLTARQARFINLELMRQPEYITGRTLERLTSLYE
                     ITRTMLPMQSTKDLTESWLESLFRCLPVDCGAILLLNPVTQELELVAQRNRDLTHSES
                     IKVSRTIVAQTFQDNVAIRSTDASTDERFSAQQSVIIQRVSSVLSAPINSKKRVWGVC
                     YLYSHMPGLFDSEDLEFVMATAQEAGLVIENIRLTEELRATQEQLVRSERLATIGKIT
                     SAISHELRNRLALLSGIEVIQKKYADDPEVSRFAEMVITGQRRALALVEEIREFARNR
                     PVEYEKSARPIVPTLEKTVSLLRLDPIISNMPSIASYEGAT"
     gene            complement(1628..>2179)
                     /locus_tag="PKZ53_16105"
     CDS             complement(1628..>2179)
                     /locus_tag="PKZ53_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNJ42012.1"
                     /translation="AAAIGYSAESKINFRFGLVRNHYVGRTFIEPQQAIRHFGVKVKL
                     NPVRDLLEGKRVILVDDSIVRGTTSRKIVEMVRRAGATEVHMRISCPPTISPCYYGVD
                     TPTYKELIAFNSTIEEIREFINADSLGYLSHEGLLTACGADEGLSFCSACYTGKYPLQ
                     TPHQSQAEKARVLAHSKQVASRE"
BASE COUNT          504 a          520 c          604 g          551 t
ORIGIN      
        1 gtcgcgccct cgtacgacgc gatcgacggc atgttcgaga tgatcggatc gagccggagc
       61 agggaaacag ttttttcaag cgttggcaca atcgggcgcg cacttttttc atattcaaca
      121 ggccggttcc gggcaaattc gcggatttct tcaaccagcg ccagcgcccg tctctgaccg
      181 gtaatcacca tttctgcaaa gcgactgact tccggatcat ccgcatattt cttttgaatg
      241 acttcaatgc cgctcaaaag cgccagccgg ttacgaagtt cgtgtgaaat tgctgacgtg
      301 attttcccga tggtggccag ccgttctgat cggacaagtt gttcctgggt tgcgcgtagt
      361 tcttcggtca ggcggatatt ttcgatgacg aggcccgctt cctgcgccgt ggccatgaca
      421 aattcaaggt cttcggaatc aaacaggccc ggcatatggc tatagaggta gcacaccccc
      481 cagactcgtt ttttcgaatt gattggggct gacagcacgg aggaaactcg ctgaataatc
      541 acgctttgct gggctgaaaa tcgctcgtcg gttgaggcat cggttgagcg gatggcgaca
      601 ttgtcctgaa acgtctgggc gacgatggtt cgactgactt tgattgattc actgtgggta
      661 aggtcgcgat tgcgttgcgc caccagctcc agttcctggg tgaccgggtt gagcagcaga
      721 atggcgccgc aatcaaccgg aagacaccga aacaatgact caagccagct ttcggtgaga
      781 tccttggtgc tctgcatcgg gagcatggtg cgggtgattt catagagtga ggtcaaccgc
      841 tccagtgtcc ggccagtgat gtattctggc tgacgcatca gttccaggtt gatgaaccgg
      901 gcctgacgag ccgtcaatct cagatcaatt cgcgaggtgt caattgggtt taaactgctg
      961 gtggaaacgt aggctggata ttcaaacttc aatttggcga tgctggggtc acctaaaata
     1021 atttcatcgc cgggttcgag aagatattcg ttgatgcgct cgcctttgat gattgtccca
     1081 actttgctgc ctttatcccg cagcaggtat tgtccttcag caaccagtat aatttcagcg
     1141 tgcatgcgtg aaatcagggt gtcgctgatc tgcaagtcat tgtcggtacc gcgcccaatc
     1201 gaaaatactg accctggaat ctcgatcttg gagggattca tgcttctgcc cgtcacaatc
     1261 aagtagggct tgcgcagctc agtttgaagt tcatccatac gtgccttgcc tggaaccaat
     1321 aagcgaacag gcgctcgctg tggttgtgaa atgttttttg aatcaacaga aacgagaagg
     1381 gtgtctcggg atcattttgg tcagtgaaga aagatcatta tacaacaggg gcggcaaaat
     1441 acacggcggc tttgagataa atgagagata ggggataggg ggtaggggat aggggataga
     1501 ggatggggaa tcgagagagt aagtaagccc ttttgccatg atcatatcgt atgagcaaaa
     1561 ggctaaatta ttgataaaac tgtgtttccc tcagctctta gccctatacc ctcagcccaa
     1621 caaggtctta ctcgcggctt gcaacttgct ttgaatgagc caaaaccctg gctttttcag
     1681 cctgtgattg atggggagtt tgcaatgggt atttgccagt gtagcaggca gagcagaatg
     1741 acagaccttc gtcggcgcca caggcggtca aaagcccttc gtggctcaga tagcccaggc
     1801 tgtcggcgtt gataaattcg cggatttcct cgatggtgga attaaaggca atcaattctt
     1861 tgtaggtcgg tgtatctacg ccgtagtagc acggcgagat ggttggcggg caactaatcc
     1921 gcatatgaac ttcggtggcc ccggcccggc gcaccatttc gacaatcttt cggctggttg
     1981 tcccgcgcac aatcgaatca tccaccagaa ttacgcgttt gccttcgagc aagtcacgga
     2041 ctggattgag tttgactttg acgccaaagt gccggattgc ctgttggggc tcaataaacg
     2101 tccggcctac atagtggttt cgaaccaggc caaatcggaa attgattttt gattcggctg
     2161 agtacccaat cgcagcggc
//
LOCUS       DAOQDO010001084         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47589_length_2179_cov_2.663842, whole genome shotgun sequence.
ACCESSION   DAOQDO010001084 DAOQDO010000000
VERSION     DAOQDO010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47589_length_2179_cov_2.663842"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1063)
                     /locus_tag="PKZ53_16110"
     CDS             complement(125..1063)
                     /locus_tag="PKZ53_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42013.1"
                     /translation="MAKKTVKKIGVLVGRERSFPDAFIAEVNSRNAGVVAEYCSLGGT
                     IASNTSEYAVIVDRISHEVPYYQTFLKHEALNGCHVINNPFWKLADDKFFGTAMAEKI
                     GVAIPRTVALPSHSYIADITAESLSNLKFPIDWQAIVDYTGFPAYMKPHWGGGWKHIY
                     KVNSLPELWKAYNQTDTLCMILQESIEWDQYVRCICIGKKHVLITNWDPRKPHHLRYD
                     ASTPLPLEPALEKRVVADVIKLCEALGYDMNTVEFAVRDGIPYAIDFMNTAPDFALES
                     IGETYFKWVIDTMADYTIECAKKGVKKPTYRWDALL"
     gene            complement(1245..1907)
                     /locus_tag="PKZ53_16115"
     CDS             complement(1245..1907)
                     /locus_tag="PKZ53_16115"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNJ42014.1"
                     /translation="MTRFAFLLPGIVNKAKAWVASLSQWLIGFGAFGIFALTLIDSAL
                     IPTAGAPDLAIVTFSLAKPDQWWVFAIAAALGSTIGCFILYSLAQKAGEKLLASVSQD
                     RRARIENLLGRWDLLTLIVASLLPPPFPFKPFIICAGAVRFNRFRLFIGLLIGRGIRY
                     GVLAYLARYYGEQALKLIAQNSGWIFLGVGILAVGVWLYQRFFSGKNNVIPPSEVHSL
                     GD"
     gene            complement(1952..2029)
                     /locus_tag="PKZ53_16120"
     tRNA            complement(1952..2029)
                     /locus_tag="PKZ53_16120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1992..1994),aa:Pro,seq:tgg)
BASE COUNT          597 a          506 c          563 g          513 t
ORIGIN      
        1 gatcacgcgc agggtggccg ccgttacctc aggtccaatt ccgtctccgg gaattaaagt
       61 gattgtatgt cgcataaagt attctttcgg agtgcggcgg cttgacgccg cttttcaaaa
      121 aagtttacag cagtgcatcc cagcgatacg tcggtttttt aacacctttt ttggcgcact
      181 cgatggtgta atcagccatc gtgtcaatca cccatttgaa ataggtttcg ccgatggatt
      241 caagcgcaaa atcaggtgcc gtgttcataa agtcaatcgc atagggaatg ccgtcgcgga
      301 ccgcaaattc aaccgtgttc atatcatagc cgagggcttc acacagtttg atcacatcgg
      361 ccaccacgcg tttttcaagc gctggctcaa gtggaagtgg ggtgctggcg tcatagcgaa
      421 gatggtgtgg tttgcgggga tcccagttgg tgatcaatac atgttttttg ccgatgcaga
      481 tgcagcgcac atactgatcc cattcaattg actcctgcag gatcatgcac aacgtgtcgg
      541 tctggttata ggctttccac aattccggca atgaatttac tttataaata tgcttccaac
      601 caccacccca gtgtggcttc atataggccg gaaaccctgt gtaatcaaca attgcctgcc
      661 agtcaatcgg gaatttgagg tttgacaggc tttcagccgt gatgtcggca atgtaggaat
      721 gtgacggcaa cgcaacggtt ctgggaatcg caacgccaat tttttcggcc atcgcggtcc
      781 cgaaaaattt atcatccgcc agtttccaaa atggattgtt gatcacatgg cagccattga
      841 gggcttcatg tttcagaaat gtttgataat aaggaacttc gtgggaaatg cggtccacaa
      901 tcaccgcata ttcgctggtg tttgaggcaa tggttccgcc cagggaacaa tattcagcca
      961 cgacaccggc attgcgtgag ttgacttcag caataaatgc atccggaaat gaacgttcgc
     1021 gtccgaccag gacaccaatt ttcttaactg tctttttagc cacgtgaatt tctccgtttc
     1081 agattgaatg ggatagttag aggctggaaa gacgtgacgc tggaccaaat gaaaaccgac
     1141 ctggaacggt gtgccaccac tggcccgccg tgaaatgcag gtataaaaga gtatgagtgc
     1201 agtcagtata tgagtggtct ggttttgtgt gatcaagcag cgtttcagtc acccagcgaa
     1261 tgaacttcag aaggggggat tacattgttt ttgcctgaaa aaaaacgctg atagagccag
     1321 actcccacgg ccagaatacc aactccgagg aagatccagc cactgttttg ggcaatcagt
     1381 ttcagcgctt gttcaccata gtatctggcc aggtaggcca gtacaccata gcggattcca
     1441 cgtccaatca atagaccgat aaacaggcga aaccggttaa accgcaccgc accagcacaa
     1501 atgataaagg gtttgaatgg aaaaggcggt ggcaagagtg aagccacaat caacgtcaga
     1561 agatcccagc gacccaggag attttcaatt cgggcgcgac gatcctggct gactgaggcg
     1621 agcagttttt caccagcttt ttgtgccaga ctgtagagga taaaacaccc aatcgttgag
     1681 ccaagggcgg cagcaatcgc aaaaacccac cattggtcag gtttagcaag tgaaaatgtg
     1741 acgattgcga gatcgggagc accagcggtt ggtatgaggg cggaatcaat gagggtaaga
     1801 gcaaatatcc caaaagcgcc aaatccaatg agccattgac tgagagaggc aacccaggcc
     1861 ttcgccttgt tgacaatccc cggaagtaag aatgcaaaac gggtcataga gtacagagga
     1921 ggaaaattga cgccgggaga agaaagctaa atggtcgggg cgagaggatt cgaacctccg
     1981 acctcttgat cccaaatcaa gcgcgctagc caggctgcgc cacgccccga aaaatcaaaa
     2041 atgcagatgg aaaagcgaag tcgccctttg tagccggatt ggtgtctgat gtcaagcact
     2101 aaatacttgg agggcttagg gcttagggct tagggcttag ggtaaaatgt ctattttcaa
     2161 taaattagct tgatttcca
//
LOCUS       DAOQDO010001085         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47630_length_2178_cov_2.631653, whole genome shotgun sequence.
ACCESSION   DAOQDO010001085 DAOQDO010000000
VERSION     DAOQDO010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47630_length_2178_cov_2.631653"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="PKZ53_16125"
     CDS             complement(<1..2078)
                     /locus_tag="PKZ53_16125"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HNJ42015.1"
                     /translation="MSSARGPVQHSYQEFQPMVVSQWNYVPRFSKKWLVKGFLIFLTG
                     WVAFQPGVVLAQSPGDLHQEPQLTELEKKLLTVPSPERARTTLRAYTAKPHLAGTPED
                     YETARFTQEEFRKAGLQAELVEYEAWMPYPKSRLVEIVAPVKFTASLTEPVQPEDFDS
                     KTLRSVPTYSAYSPSGDVTADVVYANYGLPQDFDALQRLGVDVKGKIILVRYGAAYRG
                     VKAKVAEERGAAGVLIFSDPKDDGFVRGETFPNGPFRPDTCVQRGSINYIFNYPGDPL
                     TPGVAATKNAKRIQPIEAKNLPRIPVQPLSYRDALPILENLGGQAVPEGWQGGLPIPY
                     RTGPGPTKVHLKLQMEYKSRKIWNVIGTIPGKSQPDEWVLLGNHRDAWVYGAVDPSSG
                     STALLEVARSFGELLKQGWRPHRTLKLCSWDGEEFGLIGSTEWAEDNAAVLKEKAIAY
                     LNVDVAVCGGNFGSSAVPSLWPLLRGASRVVLDADGKQTLHGSWIARNPDAIEIGIGQ
                     LGGGSDFVPFLNHLGIPAADMGMGGPYGVYHSTYDSFFWMEKFGDPGFTRHATMARMW
                     SLVAYRLVDLPTIQLNLSEYAHELLRLCFDLEKSFKETPGKPEFTQLNLAATQLVLKA
                     EAFERSGDTPATLAARNRKRRQFERALTDEKGLPGRPWFRHLVYSPGVYSGYGAEVFS
                     GIRHALNQKRP"
BASE COUNT          537 a          586 c          553 g          502 t
ORIGIN      
        1 ggtcgttttt ggttcagagc gtgtcgaata ccagaaaaga cttcggcgcc atatccggaa
       61 taaacaccag gcgagtacac cagatgtcgg aaccatggtc gtccaggaag tcctttttca
      121 tcagtcagag cgcgttcaaa ctgtctccgt tttcgattac gggcagcgag cgtggccggg
      181 gtatcaccgc tccgttcaaa agcctcggct ttcaaaacaa gttgcgtggc cgccaggttg
      241 agctgggtaa actctggttt gcccggcgtc tccttaaagc tcttctccag atcaaaacac
      301 aatcgaagaa gttcgtgggc atattcactc agattgagtt gaatcgtcgg taaatcaacc
      361 agccgataag ccaccagcga ccacatgcgg gccatggtcg cgtgccgggt aaagcctgga
      421 tcaccaaact tctccatcca gaaaaaactg tcataggtcg aatgatagac gccatatggc
      481 ccgcccatcc ccatatcagc cgctggaatt ccaagatggt tgaggaatgg cacaaaatct
      541 gacccaccac ccagttgccc aattccaatt tcaatcgcgt caggattgcg ggcaatccag
      601 cttccgtgca gcgtttgctt gccgtcggca tcaagcacaa cccttgatgc acctcgcagc
      661 aacggccaaa gacttggaac cgcactgctt ccaaaatttc caccacacac cgccacatca
      721 acattcaaat aggcaattgc tttttctttg agcacagcgg cattgtcttc tgcccattca
      781 gtcgaaccaa tcaacccaaa ttcttcgcca tcccagctac acagcttgag tgtccggtga
      841 ggtcgccagc cttgtttgag cagttcccca aaagatcggg caacttcaag caaggcagtt
      901 gaaccactcg aaggatcaac cgccccatac acccaggcat cccggtgatt tccgagcaat
      961 acccattcat ctggctggct tttccctgga atcgttccga tgacattcca gattttgcgg
     1021 gatttgtact ccatctggag tttgagatga actttggtgg gacccggacc ggttcgatat
     1081 ggaatcggaa gccctccctg ccagccttcc ggaactgcct gaccacccag gttctctaaa
     1141 attgggagtg catcccgata ggaaagcggc tgaactggaa ttcgcggcag gttcttagct
     1201 tcgatgggtt gaatgcgttt tgcatttttt gtggcagcaa caccaggtgt gagcgggtca
     1261 cccggatagt taaaaatgta attgatacta ccacgctgaa cgcaggtatc gggccgaaat
     1321 gggccatttg gaaaggtttc cccacggaca aagccgtcat ctttggggtc tgagaaaatc
     1381 aaaacaccag cggcgccacg ttcttcagcc actttggctt tcacgccacg atacgctgcg
     1441 ccataccgaa ccagaatgat tttccctttg acatcaacac cgagccgttg cagtgcgtca
     1501 aaatcctggg gcaagccata gttagcatac accacgtcag cagtgacatc gcccgagggt
     1561 gaataggccg aataagtcgg cacggagcga agtgttttgg aatcaaagtc ttctggctgc
     1621 acgggctcag ttaaagaggc agtgaatttt accggtgcca caatttcgac cagacgcgat
     1681 ttcggatagg gcatccaggc ttcatattca accagttcgg cctgcaaccc agcctttcga
     1741 aattcctcct gtgtaaatcg tgcggtttca tagtcttcag gcgtgccagc cagatggggt
     1801 tttgccgtgt aggcacgcaa tgtcgttcgc gcccgttcag gggatgggac agtcagaagc
     1861 tttttctcaa gctcggttaa ttgtggttcc tggtgcaagt cacctggtga ctgggccagc
     1921 acaactcctg gttggaaggc aacccacccg gtcaaaaaga taagaaatcc cttcaccagc
     1981 catttctttg aaaaacgggg cacatagttc cactggctga caaccatggg ctgaaactcc
     2041 tgatatgaat gttgaaccgg gcctcgggct gaagacattg ggctgaagac atcgggcttg
     2101 gggctgaaga cattgggctg aagacatcgg gcttggggct gaagacatcg ggcttggggt
     2161 tgaagacatg gggtaaaa
//
LOCUS       DAOQDO010001086         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47646_length_2177_cov_3.964656, whole genome shotgun sequence.
ACCESSION   DAOQDO010001086 DAOQDO010000000
VERSION     DAOQDO010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47646_length_2177_cov_3.964656"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PKZ53_16130"
     CDS             complement(<1..1662)
                     /locus_tag="PKZ53_16130"
                     /inference="COORDINATES: protein motif:HMM:NF025697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HNJ42016.1"
                     /translation="MKRILSSALAFLIFLSLPTFPQFQNTFIAPPSAQAEPSRFEREF
                     IFAVTTHNHLIRFSSLEPGKILSEVSIAGLQPDESILGIDVRPSTGQLYALGSTNRLY
                     LVNPATGMTTPVMAEALASTGNEIGFDFNPVRDQIQIFDSANQSLGLDPSNGSRASAG
                     VPLAFASSDRHRNSALQPAAVAYTSSFAGAVVSSMYGIDPTLDIVFRQSSIESSLDTP
                     ESGQLLTVGRLGLDAGGLIGFDIGESNATGYASFLSPGDTKPSLYHINLQNGHASYIG
                     EIGGTEPVRDVAVGQHVTIFATNEANQLLSFLNTNPSDILTRVPITGLQAGERVLGID
                     FRPATGQLFGLGSTSRIYTINPTTGQATQVGTQPLTPLLEGEFFGFDFNPVVDRIRIT
                     GSDTQNLRVVPDTGAVAAVDGALAFAEGDVNTLKPRIVGSAYTNNVPGTTTTMLYGIA
                     ANLNCLVLQNPPNDGKLTTVGDLGFNIGDNVGFDIAPADGTAYASMIVSDCIFGTDLF
                     SINLATGVATEIGLIGFNESITGLAIGSSSATNMTTSVVINMSDAG"
BASE COUNT          589 a          495 c          574 g          519 t
ORIGIN      
        1 accggcatcc gacatattga tgaccactga ggttgtcata ttcgttgccg aactgcttcc
       61 gatggcgagg cccgtgatag attcgttaaa cccaatcaat ccaatctcgg ttgcaacacc
      121 agtggcaagg ttaatcgaaa acaaatcagt gccaaaaatg cagtctgaca caatcatcga
      181 ggcataggcg gtgccatccg ctggtgcaat atcaaaccca acgttgtcac caatattgaa
      241 gcccaaatca ccaactgtgg tgagttttcc atcatttgga ggattttgaa ggaccagaca
      301 atttaaattt gcagcaatcc catacagcat ggtcgtggtt gtccccggaa cattgttggt
      361 ataggccgac ccaacaatcc tgggtttgag cgtgttgaca tcaccttcgg caaacgccag
      421 ggcaccgtca accgcagcga cagcgccggt atctggaaca actcgaaggt tttgcgtgtc
      481 actgcccgta attcggatcc gatcaaccac cgggttaaaa tcaaatccaa agaattcgcc
      541 ctccagaagt ggagtcagtg gctgggtgcc cacctgggtg gcctgccccg tggttgggtt
      601 gatggtatag attcggctgg tgctgccaag tccaaacaat tgcccggttg ccgggcgaaa
      661 atcaatcccc agaacgcgtt ccccagcttg taacccggtt atcggaacgc gagtgagaat
      721 atcgctcgga ttggtattga gaaaagaaag aagttgattg gcttcgttgg tggcaaaaat
      781 cgtaacgtgc tggccaactg caacatcccg aactggctcg gtgcccccga tttcaccgat
      841 ataactggcg tgtccatttt gtaaattgat gtgatagaga ctgggtttgg tatcgcccgg
      901 cgaaagaaac gaggcatacc cggtggcgtt gctctctccg atatcaaatc caatcaatcc
      961 gccagcatcg agtcctaatc tgccaacggt gagaagctgg ccagattcag gcgtatctaa
     1021 cgaactttca atactgctct ggcggaacac aatgtccagg gttgggtcga tcccatacat
     1081 tgagctgacc acggcgccgg caaagctact ggtataggcc acagcggcgg gttgcagggc
     1141 tgaatttcga tgacggtcac tggaagcaaa cgccagtggg actccggcac tggcccggct
     1201 gccgttgctg gggtcaagtc ccagagactg gttggcggaa tcaaaaatct ggatttgatc
     1261 ccgaaccggg ttgaaatcaa accctatctc attgccagtg ctggccagtg cctcagccat
     1321 gaccggagtg gtcattccgg tggccgggtt gacaaggtag agccgattgg tgctgccaag
     1381 cgcatagagt tgtccggtcg aaggacgaac atcaattccg aggattgact catccggttg
     1441 cagcccggcg atggaaactt cagataaaat tttgcctggt tcaagtgaag aaaatcggat
     1501 caaatggttg tgtgtggtca cagcgaaaat aaattctctt tcaaatctgg aaggctcagc
     1561 ctgagctgat ggtggcgcaa tgaaagtatt ctgaaattga gggaacgtgg gcaagctcaa
     1621 aaatatgaga aacgccaggg ccgaagacag gatgcgtttc atagggatct cctcctttga
     1681 caaggtgata ggggcaggtt gattgggtgg gccatcaaaa ggagaaagcg agttgtaaac
     1741 caaagtgcct ggttaaatcg gctctcaatg gaaccaacca ttcctatcat aagagcccca
     1801 ggtatcaata actgttcaaa attggacatc cctgcaaagg ttgaaagaaa aagatggttg
     1861 ccggcttatt gctgaggatc gtgtgcgagg gatggatggt ttcctggaaa tatggcagtt
     1921 cagataaaga aaccacggaa tacacggaat acacagaaaa taaatccaaa tttttcaata
     1981 gtttctgagg cttcctaaaa tataaattca ggaaatattt atctgaaaaa ctatagcatt
     2041 tcagaaaatg atttcggttc agagtatcac cttcaggtgt gaggcgttac gagtggggca
     2101 agaggtaagt accttgtcat aaatttcctg agatattggg tttggtaagt gcttgatttt
     2161 tttccgtgtt ttccgtg
//
LOCUS       DAOQDO010001087         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47655_length_2177_cov_3.083883, whole genome shotgun sequence.
ACCESSION   DAOQDO010001087 DAOQDO010000000
VERSION     DAOQDO010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47655_length_2177_cov_3.083883"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKZ53_16135"
     CDS             <1..901
                     /locus_tag="PKZ53_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNJ42017.1"
                     /translation="FPLIKNGLLINLTSGAVTQREEVIEVFEVKTGVDLYTMSVDRAK
                     WNATHPGKEFKSLEFCEDGRMVVMTTSATCFVADFRGRSIKELSFSPAGPPESAECVE
                     NTLYLTGKTGVATFVQAIDVATGKPLWTRLGDRPVELVPSLAAGKGLLLEGGEAGKPS
                     RLVASDLQTGKDLWATEIPRASLSGISTGPAGAGLIVVRPDQEPRLLTIEVETGKIKY
                     NLQTSDFPARSLLNAKPPYVAVISEKAQGSESEAKVTVFDLQTGTYLWNHIDDPFYYL
                     DFTSEYLLVGNTSYILAFPLQKK"
     gene            1052..2146
                     /locus_tag="PKZ53_16140"
     CDS             1052..2146
                     /locus_tag="PKZ53_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42018.1"
                     /translation="MEHLERDPELTMALKAFFIEKLPWKVTEPLFGSYLAFTFAELNH
                     QVPTPVESHTQRAVVGATNSPFYPSGSESANHHQNQPAGSKEVFEVAERIVQLLQKAI
                     SQFESDYAENFQKLSSLFRWRRASDPENLASETLKRAYLNIIRGYRENWKLIEENGVV
                     KCLYTEDQNSFELKNYIYQISNKVNLEAYRKIDISESLDNQFEFQDRGIESAEPGLSA
                     SLHEEYRLTVEHLIAVANKCLSCLRQCKFQNAHFTGSEMYSILVRYITISELSQEEQT
                     VRFREFATELGISINHLRTLVTYTKHRVARCMQLNQSKGRRATPTCQDLKMPNADESA
                     ADAFQRFRAGLDALIDQIGIKEEGRMKNEE"
BASE COUNT          594 a          515 c          540 g          528 t
ORIGIN      
        1 ctttccattg atcaaaaatg gattattgat caatttgacc agtggtgccg ttacccagcg
       61 cgaggaggtc atcgaggtct ttgaggtcaa aaccggggtg gacctctata ccatgtcggt
      121 tgaccgggca aaatggaatg ccacgcatcc aggcaaggaa ttcaaatcgc ttgaattctg
      181 cgaagatggg cgaatggttg tgatgaccac cagtgccacc tgttttgtcg ctgattttcg
      241 ggggcgatct atcaaagaac tctcattttc accagccggc ccccctgaat cagccgaatg
      301 tgtcgaaaat accctctatc tcaccgggaa aaccggagtc gccacgtttg ttcaggccat
      361 tgatgtggca actggcaagc ccctgtggac ccgcctcgga gaccgaccgg tcgaactggt
      421 accatcgctg gcggcgggga aagggttgct gctcgaaggc ggggaagctg gaaaacccag
      481 ccgcctggtc gcttctgatc ttcaaaccgg aaaggacctc tgggcaactg agattccacg
      541 ggcaagcctg tccggtattt ccaccggacc agccggtgcc gggctgattg tggttcgacc
      601 cgaccaggaa cccagattgc tgacgattga agttgagacc ggaaaaatca aatacaatct
      661 ccagacgagt gactttccgg cacggtctct gttaaacgca aagccgccat atgtggctgt
      721 catttctgaa aaagctcagg ggagcgaatc cgaggcaaag gttacggtgt ttgatcttca
      781 gaccggaacc tacctttgga atcacattga cgatcctttc tattacctcg atttcacctc
      841 tgagtacttg ctggtgggca ataccagcta cattctggcg tttccgcttc aaaaaaagtg
      901 agtggctgac aggcggaaga ggctgacaaa agctatttgt ttatttttcg ggaatcgggc
      961 tacactttgg gttctgtctt ttttgcgtat ctttggaatt tctgcctgta attccatcaa
     1021 tttggtggtc atttgaatga gtacattgcc catggaacat ctggaacgag acccggaatt
     1081 aacaatggcc ctgaaagctt tctttattga gaaattgccc tggaaagtga ccgaacctct
     1141 ttttggttcg tacctggcct tcacgtttgc agaattaaat catcaggttc caacaccggt
     1201 tgaatcacac actcaacgtg ccgttgtcgg agcaacaaat tcaccctttt acccatctgg
     1261 gagtgagtca gcaaatcatc atcaaaatca accggcggga agcaaagaag ttttcgaagt
     1321 tgccgagcgc attgtccagt tgctccaaaa agcaatttcc caatttgaat cagattatgc
     1381 tgagaacttt cagaaactaa gcagtttatt tcgatggaga cgcgcatcag accctgaaaa
     1441 cctggcgagc gagaccttaa agcgagccta tctcaatatc atccggggat accgggagaa
     1501 ctggaaatta attgaagaaa acggcgttgt aaaatgcctg tatactgaag accaaaatag
     1561 ttttgaatta aaaaattata tctatcagat ttcgaataag gtcaatcttg aagcctatcg
     1621 caaaattgac atttcggaat cgcttgacaa ccagtttgaa tttcaggacc gggggattga
     1681 atcggctgaa ccgggactca gcgcctcact gcatgaggaa taccggctca cggttgagca
     1741 tctgattgcg gttgccaaca agtgcctgtc gtgcttgcgg caatgcaaat ttcaaaatgc
     1801 tcatttcacc ggcagcgaaa tgtactcaat tttggtgcgc tacatcacga tcagcgaatt
     1861 gtcccaggag gaacaaaccg ttcgatttcg tgagtttgcc accgaacttg gaatcagcat
     1921 caatcacctg cgaacactgg tcacctatac caaacatcgg gtggcccgct gcatgcagct
     1981 caatcaatcc aaaggccggc gggcaacacc cacctgtcag gatttgaaaa tgccaaatgc
     2041 tgatgaatcg gctgccgatg cgtttcaacg atttcgagcc ggtctggacg cgctcattga
     2101 ccagattggg atcaaggaag aaggaagaat gaagaatgaa gaatgaagaa tgaagaatga
     2161 agaatgaaga atgaaga
//
LOCUS       DAOQDO010001088         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47700_length_2176_cov_3.083923, whole genome shotgun sequence.
ACCESSION   DAOQDO010001088 DAOQDO010000000
VERSION     DAOQDO010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47700_length_2176_cov_3.083923"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="PKZ53_16145"
     CDS             complement(<1..2131)
                     /locus_tag="PKZ53_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNJ42019.1"
                     /translation="MTDPTPSGPLSVTNLPEAEHALRTYRMRQTRILILSIITLMFLG
                     VLIAQGSLDLLNLISPSTPVATFILYGLSMLNFMASMLLLFVLSRNILKLIQERRQRK
                     LGSKFKSQMVLGSLAISILPVVMLFFFAYSLVNRSLEKWFNIPAQRFEEDVEFIQDQM
                     YSQEMKALSEVGRSMIRLLKMRQMAVLPGADTTPSRLDLDTTGREVFKAEFRSHELYR
                     LFMLDSDGKQVLELGDSFFDGLSNVQQAKSGVARNDPHSSIFVTEDNRMFVLVGLPIR
                     AGVVAGNGKKTVPQAGSRQPKQVGGLFLIRQLPPSLINRFDQLSNEARNRADLTIQNR
                     RMRFTYLYILGFVTLLLVFGTTWIALYIARGITVPIQALAEGTHHVAQGNFDYVVNVA
                     ADNELAVLVNSFNDMASQLRTNRHQLEATATQLRTMNRALEERKQYIETILQSLFTGV
                     VSLDAEQRVTTINQAGIELLRLDQSSIGLPVEQAVPAANRVEFQRVLRRTRRLGQSTV
                     EVELKIQDQKPLHVAVSGTALRDEQGKYKGAVLMIEDLSPLIQAQQSAIWSEVARRMA
                     HEIKNPLTPIQLCAERISRNIDRHGVDSERVRQTIHECTATISREVATLKRMVDEFSR
                     FAKLPDAQPEPSSLNQVVREAIRLYDDRLDDIELETSLDETVPVLNIDREQFKRTLVN
                     LIDNARHAIEAAADRSGRSHDHHGIRHL"
BASE COUNT          546 a          572 c          529 g          529 t
ORIGIN      
        1 ccagatgacg aattccgtgg tggtcatggg atctccctga tcggtcagcc gccgcctcga
       61 tggcgtgtcg tgcattgtca atcaaattaa ccagggttcg cttaaattgc tctcgatcaa
      121 tgttgagaac tggcaccgtc tcatcgaggc tggtttcaag ttcaatgtca tcaagccgat
      181 catcataaag acgaatggct tctcgaacca cctgattgag cgatgatggt tctggctggg
      241 catccggcaa tttggcaaac cgtgaaaatt catcaaccat gcgtttcagg gtggcgactt
      301 cccggctaat ggttgctgtg cattcgtgga ttgtctgacg aacccgttct gaatcaaccc
      361 catgccgatc aatattgcgg ctgatgcgct cagcacagag ttgaattggc gtcagtggat
      421 ttttgatttc gtgagccatc cggcgggcaa cttcgctcca gatcgcgctt tgctgtgcct
      481 ggatgagcgg cgaaagatct tcaatcatca gaaccgcgcc cttgtatttc ccctgttcat
      541 cgcgtaaggc agtccctgag acagccacgt gcaggggctt ttgatcctgg attttgagtt
      601 caacctcaac cgtagactgc ccaagtcgcc gcgttcggcg taaaacgcgt tgaaactcca
      661 cccgatttgc ggctggcact gcctgttcaa ccggcaaacc aattgatgac tggtcaagtc
      721 tgagcaactc tatacccgcc tggttgatgg ttgtcacccg ctgttcagca tcaagcgaga
      781 ccacacctgt aaacagactt tgcaaaatcg tttcaatgta ttgttttcgt tcttcaagcg
      841 cccggttcat agttcggagc tgggtggcag tggcttcaag ttgatggcgg ttggtgcgta
      901 gttgactggc catgtcatta aatgaattga ccagcactgc cagctcgtta tcagccgcaa
      961 cattgaccac ataatcaaaa tttccctgcg cgacgtgatg cgtcccttcc gccagtgcct
     1021 ggatcggaac cgtgattccc cgcgcaatat agagcgcgat ccaggtggtt ccaaaaacca
     1081 gcaacagtgt gacaaacccc aggatataca gataggtaaa tcgcatccga cgattttgaa
     1141 tcgtcaaatc cgctcgattc cgagcctcat ttgaaagctg gtcaaaccgg ttgatcaatg
     1201 acggtggaag ttgacgaatc aaaaacaacc cgccaacctg ttttggctga cggcttccgg
     1261 cttgcggcac ggttttcttt ccattgccag ccaccacacc ggcccgaatc ggtaacccaa
     1321 ccagcacaaa catccggttg tcttcggtga caaagattga actatgcggg tcattgcggg
     1381 ccacacctga ttttgcctgc tgaacatttg acaacccgtc aaaaaaacta tcccccagtt
     1441 ccaggacctg tttgccatca ctgtcgagca taaacagtcg gtacaactca tggctgcgaa
     1501 attcggcttt aaacacttca cgaccagttg tatccagatc caggcgtgag ggcgtggtgt
     1561 cggcaccggg caagacggcc atttgtcgca ttttcaagag ccgaatcatc gagcgcccaa
     1621 cttctgaaag ggctttcatt tcctgggagt acatctgatc ctggataaat tccacgtcct
     1681 cttcaaatcg ctgggccggg atattaaacc acttctcaag actccggttc accagactat
     1741 aggcaaagaa aaacaacatc accactggca aaatcgaaat tgccagcgac ccaagaacca
     1801 tttgtgattt aaattttgat cccagtttcc tttgacgtcg ctcttgaatc aacttcagaa
     1861 tgttgcggct gagcacaaac agcaacaaca tcgacgccat aaagttcagc atcgagagac
     1921 catacaaaat aaacgtggcc accggtgtac tgggactgat caggttcaat aaatcaagcg
     1981 atccctgagc aatcaacacc cccaaaaaca tcagggtgat gattgacaga atcaagatac
     2041 gggtttgccg catgcgatag gtacgcaacg catgctcagc ctcagggagg ttggtgacac
     2101 tgagaggtcc ggatggcgtg ggatcggtca tctttggtcg gttgaaagag tcagtagtca
     2161 gtagtcagat gttagg
//
LOCUS       DAOQDO010001089         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47704_length_2176_cov_2.770863, whole genome shotgun sequence.
ACCESSION   DAOQDO010001089 DAOQDO010000000
VERSION     DAOQDO010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47704_length_2176_cov_2.770863"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..2127
                     /locus_tag="PKZ53_16150"
     CDS             166..2127
                     /locus_tag="PKZ53_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNJ42020.1"
                     /translation="MKLFSKSKPAENKTFEEILSRRIERRSFLKGALSAAPLLVAGTS
                     LIDSTEASAQELPLDDEDLALGAAENRAATLADKLEFLPIRLNTEDRITVANGYAASV
                     LLKWGDPLTSNAPAFSLIRQTPAAQAEQFGFNCDYVGYFPLPGFTSKSSTTGLLVVNH
                     EYTDPLNMFLGYQAGNPTKDQVDIELAAHGASVVEIERTATGWRYKVGSAFNRRLTGQ
                     TEMILTGPAAGNELLKTSEDPTGTRVRGMLNNCAAGKTPWGTVLTCEENFNQYFANNS
                     KLTDADPRKRVHSRYGLTSAASERAWEKFYNRFDIEKEPNEPFRFGWVVEFDPYDPTF
                     VPRKRTALGRLKHEAATTVVAKDGRVVVYTGDDERFDYMYKFVTKNKVNLQNRAANFN
                     LLDEGTLYVAKFSDNGTGTWIPLIGGQGALAGWTQADVCINTRGAADLVGATKMDRPE
                     DIEVNPVSGKVYCVMTNNTRRATDNNPATDAANPRPANKHGHIIELTEAGDDQAATSF
                     EWEIFMLCGDPQVAADGVYYAGFSLAGMSSISTPDNITFDKRGNLWISTDGMPSTLKV
                     HDTVYAVPTAGPNRGKLKPFLSAPAAAEICGPEMTPANDTFFCAIQHPGEGGSIEKPT
                     STWPDGTTPARPSVIAVVKTSDGSKTIGR"
BASE COUNT          535 a          564 c          582 g          495 t
ORIGIN      
        1 tagcgtcgtg ggtacacgac tcaagcttgg cccttgaggg ccgagtctct tgactcagtt
       61 gatgtaactt caggttcagg cattgttcat tttccattca ggatttggaa acagctttgt
      121 gactaattct taagtgtaac caaccattac ccgagaggaa ttgtcatgaa actattttca
      181 aaatccaaac ctgctgaaaa taaaacattt gaagaaatcc tgtcgcgccg catcgagcgc
      241 cgttcatttc tgaaaggggc actttcggcg gccccattgc tcgtagctgg aacatcgttg
      301 attgattcga cggaggccag tgcccaggaa cttccacttg atgatgaaga tctggctctg
      361 ggagcggctg aaaatcgagc ggcaaccctg gccgataaac tcgaattcct tcccattcgc
      421 ctcaatactg aagatcgcat caccgttgcc aatggctatg cggcttcggt tttgctcaag
      481 tggggtgatc cactgacgag caatgccccg gcgtttagtt tgattcgcca aaccccagcc
      541 gcccaggccg agcagttcgg ctttaactgt gattatgtcg gttacttccc gctgccaggc
      601 tttacatcta aaagctcaac cactggactg ctggtggtca atcacgaata caccgacccc
      661 ctcaatatgt ttttgggcta tcaggcggga aaccccacca aagaccaggt agatatcgaa
      721 ctggctgccc acggtgcgtc agtggttgaa atcgagcgga cggcaaccgg atggcgttat
      781 aaggtcggtt ctgccttcaa ccgccgcctg actggccaaa ctgaaatgat tttgactggt
      841 ccagcggctg gaaatgaatt gctcaaaact tcggaagacc caaccggaac gcgggttcgg
      901 ggaatgctca acaactgcgc tgccggaaag acgccgtggg gcacggtact cacctgtgaa
      961 gaaaacttca atcagtattt tgccaacaac agcaagctca cggatgctga cccgcgcaaa
     1021 cgagtccaca gccgatatgg gttgaccagt gccgcgagcg aacgagcctg ggaaaaattt
     1081 tacaaccggt ttgatattga aaaggagcca aacgagccgt tccgctttgg ctgggtggtt
     1141 gagtttgacc cgtatgatcc gacgtttgtg ccacgtaaac gcactgccct gggtcgcttg
     1201 aagcacgaag ccgcgacaac agtggttgcc aaagatggcc gggttgtggt ctacaccggg
     1261 gatgatgagc ggtttgacta tatgtacaag tttgtgacca aaaacaaagt gaacctgcaa
     1321 aaccgggccg cgaatttcaa tctgctggat gaaggtaccc tctatgttgc caaatttagc
     1381 gacaatggca ccggaacctg gattccattg attggcggtc aaggtgctct ggctggctgg
     1441 acccaggccg acgtctgtat caacacccgt ggagcggctg acctggttgg tgccaccaaa
     1501 atggatcgcc cggaagacat tgaagtcaac ccggtttccg gcaaagtgta ttgtgtgatg
     1561 accaacaaca cccgccgggc aactgacaat aacccggcga cagatgccgc caaccctcgc
     1621 cctgccaaca aacacggcca catcattgaa ctgaccgaag ctggcgacga tcaggcggcg
     1681 acttcgtttg agtgggaaat ttttatgctc tgcggcgacc cgcaagtggc ggctgatggt
     1741 gtgtattatg ctggctttag tctggcggga atgagttcga tttcaacgcc tgacaacatc
     1801 acgtttgaca agcgcgggaa tttgtggatt tcaaccgacg gcatgccgtc aacgctcaaa
     1861 gttcacgaca ccgtgtatgc cgttccaact gctggcccaa atcgtggcaa actgaagcca
     1921 tttttgagtg caccggctgc ggctgagatt tgcggaccag agatgacacc ggccaatgac
     1981 acgttctttt gtgcgattca gcatccaggc gaaggtggct cgattgaaaa accaaccagt
     2041 acctggccag acgggacgac accagctcgt ccgagcgtga ttgctgtggt taagacgtcg
     2101 gatggttcaa aaacaattgg acgatagtca tgccagtttg gtgtcagtcg tcagtcgttc
     2161 gctaagtaca agtcct
//
LOCUS       DAOQDO010001090         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47749_length_2175_cov_2.846226, whole genome shotgun sequence.
ACCESSION   DAOQDO010001090 DAOQDO010000000
VERSION     DAOQDO010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47749_length_2175_cov_2.846226"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PKZ53_16155"
     CDS             complement(<1..1631)
                     /locus_tag="PKZ53_16155"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ42021.1"
                     /translation="MNSKLEVDGRGPVVEPLEGRSPFPYQLLQSKQTRGDLVQLQRHI
                     QLLDEVANLSQTLLATPNLIEGFQQVVEKLGHATNSDRVVITRLDWTPDDLECHGWQQ
                     VVTEWQRVGSVRYTTPELRRFPIRRSLSGVLRAILSGRNVFVIEECPEPFRSEQKSSG
                     VVWNVSVLIRCHSETWGIIGFDHRTPLTELNQADVAVLKTAAAAIASAIERHKSEEHR
                     RAAEVALMLEREQAAQIRAIELERKDALLRAVVEATRRMVEQDDFGCALSSAITALGE
                     LTGLDRVYVIHYDHTYRAGFFIAEWNAPDIETLATTVGWGPFHFHEYQEVFEPLMSNQ
                     PYSSPAHLKTGANAQLNLATSNKSDLFVPIFLDGVFWGAIGFDDCHTERFYSDGEIQV
                     LRGAATAISSALHRREIERQRTQAERAILVSREQAARGLADELAKANAAIRRSVTHLT
                     SMDNLNDFLNAVLVESIQASGAVSGAIFVYDPGSQALYIQAQVIRGELIDIHQDARNW
                     VFQEPIQVDGDLVWEIMARDRQVLWADYPRPQTTYL"
BASE COUNT          527 a          569 c          573 g          506 t
ORIGIN      
        1 agataggtgg tctgcgggcg tggatagtca gcccagagca cctggcgatc acgggccata
       61 atttcccaca ccaggtcgcc gtcaacctga atcggctctt gaaataccca gtttcgagca
      121 tcctggtgga tatcaatcaa ttcgccccgg atcacctggg cctggatgta gagggcctgc
      181 gaacctgggt catacacaaa gatcgcgccg ctcactgctc cgctggcctg gattgattcg
      241 acgagcaccg cattgagaaa atcattgagg ttgtccatcg acgtcaggtg cgttactgat
      301 cgccggattg cggcatttgc tttggcaagc tcatctgcca gcccacgggc cgcctgttcg
      361 cgggaaacca ggattgctcg ttctgcttga gttcgctgcc gctcgatttc ccggcggtgg
      421 agggctgatg aaatggccgt cgctgcccca cgtaagacct gaatctcgcc atccgagtaa
      481 aaccgttcag tgtggcagtc atcaaaccca atcgcccccc aaaatacccc atccagaaaa
      541 attgggacaa agaggtcact tttattgctg gttgcgagat tcaactgtgc attggcacca
      601 gttttgaggt gagccggtga agagtatggc tggttggaca tcagtggttc aaaaacttcc
      661 tgatattcat gaaaatgaaa tggcccccag ccgacggttg tcgccagggt ttcaatatcg
      721 ggagcattcc actcggcaat gaaaaatccg gcacggtagg tatggtcata gtggattaca
      781 tacactcgat ccagtccggt taattcaccc agcgccgtaa tcgccgaaga cagagcgcac
      841 ccaaaatcat cttgctcaac catgcgtcgt gtggcttcaa ccaccgcccg aagcaaggca
      901 tccttgcgct caagttcgat ggctcgaatc tgagccgctt gttcacgttc aagcatcagg
      961 gcgacttcag ccgcccggcg gtgctcctcg ctcttatggc gttcgatagc gctggcgatg
     1021 gcggcggcgg cggttttgag gaccgcgaca tcggcctgat tcaactcagt gaggggggta
     1081 cgatggtcaa atccaatgat gccccaggtt tcagagtgac atcgaatcaa cacactgaca
     1141 ttccagacca cacctgaact tttttgttct gagcgaaacg gctctgggca ttcctcaatc
     1201 acaaacacat tgcgaccact caggatcgct ctcaacaccc ctgacagcga gcgtctgatc
     1261 ggaaaacgcc gaagctctgg tgttgtgtat cgaacgctac ccactcgctg ccattcagtt
     1321 acaacctgct gccatccgtg gcattccaga tcatctgggg tccaatcaag tcgggtaatc
     1381 accacccggt cagagttggt ggcgtgtcct agcttttcaa caacctgttg aaatccttcg
     1441 atgagattgg gggtggcaag cagggtttgt gaaaggttgg ccacttcatc cagcagttga
     1501 atatggcgtt ggagctgaac taaatcgcct ctggtttgtt tgctctgaag gagttggtaa
     1561 ggaaagggag accttccttc caacggttcg acgactggtc cgcgaccatc cacctcaagt
     1621 ttcgaattca atcttggagg tgcggaaaaa gtggcttctt gatcaaatgg ttgattcgac
     1681 ggaacgaact cacgtggctg gttcatgtcg ccctcgcgga tatgaggcaa actgaaggcc
     1741 ctcaatcatc cagagaaaat gctccactgg ttcctgtggc agagccgtta tgacaatgcg
     1801 tcggggcctg gatcttcttg tggggggaga ccgtaaaatc ctgtgaattg atataaaagg
     1861 gaaagtaatg gctaacacat agcaattatt taagttagcc tggagatact aacccctgaa
     1921 cactatgggc tgcaaggcgg atccgctgga cgggtcggat tgagcatcaa ctcgacgaaa
     1981 gtcgagttat tcgctctttc aaaagtcagg aaatttggtt ttttccgggc ggaggtgaat
     2041 ttctggcctg gaggatatcg acaagcggct gagcaatccc taatatcggt caaggctacc
     2101 cagcaaacac ccatctgcca gaataagtac cttaccgaaa atttccagac attttaacca
     2161 cggaaaacac ggaaa
//
LOCUS       DAOQDO010001091         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47783_length_2174_cov_2.697971, whole genome shotgun sequence.
ACCESSION   DAOQDO010001091 DAOQDO010000000
VERSION     DAOQDO010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47783_length_2174_cov_2.697971"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1557
                     /locus_tag="PKZ53_16160"
     CDS             259..1557
                     /locus_tag="PKZ53_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42022.1"
                     /translation="MPITPSTFVHQGNMRLGELRRLLAALESSFSKHHQNLQELEASR
                     HRALDQLTLVNLPNLSQEAFNDVRTFTGYGQFHVRNPLARIDEERRTLTQTIAQIEAD
                     PRFQRADELINPTSGELILKTEAFRRQCQPVFDAAQRYEALPHFLILIERNYGTDEYP
                     LRPWNLDYYLDWKHADEIVEQLGKENFDFCRHDYLHLRQARDEMNREVQSMDQEIAAV
                     HQLVEHHRQASYRLANLPELILQESRLALREHLSFVDRDQLFSWAQGDRSREALIKML
                     HGVEKKNDYVKQMANHQFVYEREMLQTAIAKLEKKVAKFSRPKYAGMGAIISDAEAAA
                     WLADPRQKLGERRDRFWKNYDRVIVFQEYDRYDYGRDLLWWDVMTDGRVDGSFIPEVR
                     EYYTSHPGYDPHSRDYHHAGRTLSSYDSSYPGDTGLVEVS"
     gene            1557..>2174
                     /locus_tag="PKZ53_16165"
     CDS             1557..>2174
                     /locus_tag="PKZ53_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucosyltransferase"
                     /protein_id="HNJ42023.1"
                     /translation="MRRLTIVAYAVNGSGLGHLTRVTAILRWVRRLGKLVGVQPDIYI
                     LTSSEASALAFEEGFAAFKIPSKTAIRLASIQKEDYLRLAKQWVWHSLGLLRPDILLV
                     DTFPGGSFGELFHALDGTGSKVFIHRAMKDVFSQQEVFQAMLPLYDRILVPEEPGQIR
                     TELSSRLAARTRYLGPILLRDRVELHSREKARRRLGIPEASLGIWI"
BASE COUNT          495 a          584 c          590 g          505 t
ORIGIN      
        1 cggaaaattg aggataccca actgacaaga ttttccagcg cccatccggg cgcgattcgg
       61 tgcgggcaca ggtccggtgg tgtgcccaaa gcggcaaacc accggctact ctccgtcgcc
      121 cattcgggcg ggagatgtct agcccaggcg acctttccgc aacggagatg aaattcattg
      181 tgttttcacc ctgctttcct aaaataggca ctccgttgcg atgccttcat ttttcaccag
      241 gattggaaca acacttctgt gccgattacc ccttccacct ttgttcatca agggaatatg
      301 cgcctcggcg aattacgccg gttgctggcg gctttggaat catcgttttc aaagcatcat
      361 caaaacctgc aggagcttga agcctcccgc catcgcgccc tcgatcaatt gacactggtg
      421 aatttgccca atctaagcca ggaagcgttc aatgatgtgc ggacctttac cgggtatggc
      481 cagtttcacg tccgcaaccc gctggccagg attgatgagg aacggcgtac cctcacccag
      541 accattgccc agatcgaagc cgacccacgg tttcagcgcg ctgatgaatt gatcaaccca
      601 acgtcaggcg aactgatcct gaaaactgaa gcctttcgcc gtcaatgcca gccggtgttt
      661 gatgcggcgc agcggtatga agccttgccg cactttttga ttctcatcga acgcaactat
      721 ggcactgacg aatacccgct ccggccctgg aatctggatt attacctcga ttggaaacat
      781 gcggatgaaa tcgtcgaaca gttgggcaaa gaaaactttg atttctgccg ccatgattat
      841 ttgcatttgc ggcaggcccg cgacgagatg aaccgtgaag tacaaagcat ggaccaggaa
      901 attgccgccg ttcatcaact ggtagagcac caccgtcagg caagttaccg gctggcgaat
      961 cttccagagt tgatcctgca ggaaagtcgg ctggccctgc gcgaacacct gtcatttgtg
     1021 gaccgtgatc aattgttttc ctgggctcag ggtgatcgca gccgtgaagc cctgattaaa
     1081 atgctgcacg gcgttgaaaa gaaaaacgac tacgtgaaac aaatggccaa ccatcagttc
     1141 gtgtatgaac gcgagatgct ccaaaccgcc attgccaaac tggaaaagaa ggtcgccaaa
     1201 ttttcacgcc cgaaatatgc cgggatgggc gccattattt cggatgcgga agccgccgcc
     1261 tggctggctg acccacgaca aaagctcgga gaacgtcgcg accggttctg gaaaaactat
     1321 gatcgggtga ttgtgtttca ggaatatgac cgatacgact atgggcgcga cctgctctgg
     1381 tgggatgtga tgacggatgg ccgggttgat gggtcattca ttcccgaagt tcgcgaatat
     1441 tacacttcac atccgggcta cgacccgcac tccagggatt atcaccacgc cggacgcacc
     1501 ctttcgagct atgacagttc ttatcctggc gataccggtc tggtggaggt ctcctgatgc
     1561 gccgattgac gattgtggcc tatgcggtaa acggttccgg gcttggccat ttgacccgcg
     1621 tgaccgccat tttacgctgg gttcgacgat tgggaaaact ggtcggcgtg caaccggaca
     1681 tctacatttt gacctcctcc gaagcctcag ccctggcatt tgaagaaggg tttgcggcgt
     1741 ttaagattcc cagcaaaacc gcgattcggc tggccagcat tcaaaaagaa gactacctgc
     1801 ggctggctaa acaatgggtc tggcatagtt tagggttgct gcggcctgac attctgctgg
     1861 ttgatacctt tccgggtggc tcgttcggag agctgtttca cgcgcttgat ggaacgggct
     1921 caaaggtgtt tattcatcgg gcaatgaaag acgtgttttc gcaacaggaa gtctttcagg
     1981 cgatgctgcc gctctatgat cgaattttag ttccggaaga accgggccag attcgaactg
     2041 aactttcgtc acgattggcc gcccgaaccc gatacctggg gccgatttta ttgcgtgatc
     2101 gggtggaact gcattcgcgg gaaaaggccc ggcgccggtt ggggattccc gaagcctccc
     2161 ttggcatctg gatc
//
LOCUS       DAOQDO010001092         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47786_length_2174_cov_2.475224, whole genome shotgun sequence.
ACCESSION   DAOQDO010001092 DAOQDO010000000
VERSION     DAOQDO010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47786_length_2174_cov_2.475224"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /gene="tssK"
                     /locus_tag="PKZ53_16170"
     CDS             complement(<1..542)
                     /gene="tssK"
                     /locus_tag="PKZ53_16170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNJ42024.1"
                     /translation="MGYTRKLVWAEGMRITPHHFQQWDHHVDDQLTARIQALIHYGWG
                     MSEIGINREAIANGHIVIDRLTGLLPDGMWFHLPESEPCPPARNFKEMFPPTESQLDV
                     YLAIPRRQPGSRNFQMNGQMSNPPVRFRQVVGTVVDETRGEGELQLGQALGNYQILVG
                     PEIREGFSALKIGVITRSATG"
     gene            complement(680..1033)
                     /locus_tag="PKZ53_16175"
     CDS             complement(680..1033)
                     /locus_tag="PKZ53_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42025.1"
                     /translation="MHNTLRTLKEAGFGKLLKGIVCLVALFWLGGTVSVLAQSGRKPE
                     GQPSQTKSKPAPPQEKDLSETDEEIFLDGGLSAAEGFAPNFITKRSTPLTIGKPKTNK
                     SQSKILVTPTRPIQN"
     gene            complement(1375..>2174)
                     /locus_tag="PKZ53_16180"
     CDS             complement(1375..>2174)
                     /locus_tag="PKZ53_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42026.1"
                     /translation="QLERAFHTTLSTSREAAAVGSDTLFFQGKLIGADKDALFISVIE
                     PADPHPLVVRGVMASDHSALVFRDNLKLLAQSVGLTACFIGRFLPEQPKTVALLATGS
                     FENQPPGPVDLSPETPAGFRFPTAWMAHCNLGLDRLQGSYLPPPDDAAVVMAGLFGQN
                     FQMKDENPLLQLQRRLERMVLGGKATLTPEMLTTIDRETALLERRLLPTAAILLHNLA
                     QTVCSTGRTISGGHRTVSPTALAEAWIAAMTYTQSALNAWYRGLWER"
BASE COUNT          552 a          539 c          539 g          544 t
ORIGIN      
        1 ccggttgcac ttcgagtaat cacaccgatt ttgagagctg aaaacccttc gcggatttca
       61 gggccgacca ggatttgata attcccaagc gcctgtccaa gctggagttc tccttctccg
      121 cgagtttcgt ccaccacagt gccaaccacc tgtcgaaagc ggacgggcgg gtttgacatt
      181 tgaccattca tttgaaaatt tcggcttcct ggctggcgac gtggaatcgc cagatagaca
      241 tccagttggc tttcggttgg cggaaacatc tctttgaaat ttcgagcagg cggacagggt
      301 tcactttctg gcaggtggaa ccacattcca tcgggcagga gcccggttaa tcgatcaatc
      361 acaatgtggc catttgcaat cgcttcccga ttgatgccaa tttcgctcat tccccatcca
      421 taatgaatca gcgcttgaat tcgagcggtt aactggtcat ctacatgatg atcccactgc
      481 tgaaaatggt ggggtgtaat ccgcatgcct tcggcccaga caagttttcg tgtatatccc
      541 actggttttg aacctcttca caagtaaatt ttcatttaat ttcactgtga ccagcgcaaa
      601 accagtgcca aaaaaacaaa acgggagaaa acctggctgg tttttctccc gttaagtgat
      661 cctcgacaaa taagaatgat taattttgga taggacgggt tggagtaacg aggatctttg
      721 actgtgattt gttggttttc ggctttccga tggtgagcgg ggttgaccgt ttggtaatga
      781 agtttggagc aaacccttcg gcggcgctca atccgccatc cagaaatatt tcttcgtcag
      841 tttctgaaag atctttctcc tgaggtggag cgggtttact tttggtttga gaaggttgcc
      901 cctctggttt gcgtccggac tgagcaagaa ccgaaaccgt tcctcccaac cagaacaaag
      961 cgaccaaaca tacgatgcct ttgagaagct ttccaaaacc tgcttctttc agggtacgca
     1021 atgtgttgtg catagaatct ccgtgagtat gatgaaagct gtgaagccgc gtaagcatac
     1081 ttggctcagg tgaatttgaa aagcagaata tgaatatcgt cttataaagg caaaccgtat
     1141 gccattgcct ggcagagagt gaaaaaactg aagcaatggc aaggccgatg cgcttttctt
     1201 cgacatcctg ttaactgcaa aatattttct ttcaagtact tacaaaacag gcacaaactc
     1261 aacgccactg attaaaaagt acaatgtctc atatttgtcg cctgcctggc cgaaattttt
     1321 ctggatagta gctacacagt gtacagtcac tgaacaccgg atatcctgac cagtttaccg
     1381 ttcccataag cctcgatacc aggcattgag ggcagattgg gtataggtca tcgccgctat
     1441 ccaggcttcg gccagcgccg ttggcgagac cgtccgatgg ccacctgaga tggtccgacc
     1501 ggttgaacat actgtctgcg ccagattgtg aagcagaatg gccgcggttg ggagcaaccg
     1561 ccgttcaagc agggcggttt cacgatcaat ggtggtcagc atttctggcg tcagcgttgc
     1621 tttgccacct aataccattc gctcaagccg tcgttggagt tgaagcagcg gattttcatc
     1681 cttcatttga aaattctgcc cgaaaagccc agccatcacc acggcggcat catctggcgg
     1741 cggcagatag ctcccctgca gccggtccag gccaaggtta caatgggcca tccaggcagt
     1801 tggaaaacga aaccctgccg gagtttcagg actcaaatca acaggccctg gtggctgatt
     1861 ttcaaagcta ccagtcgcaa gtaacgccac cgtcttgggt tgttcaggca gaaatcgccc
     1921 gatgaaacag gcagtcagac ccacgctttg agccagcaac tttaagttat cacgaaaaac
     1981 aagggctgaa tggtccgagg ccatcacacc acgaacgaca agtgggtgag ggtcagccgg
     2041 ttcaatcact gaaataaaca gtgcatcttt gtcagcacca atcagttttc cttgaaagaa
     2101 cagcgtatcg ctgcccacag cggcggcttc cctggatgtg gataaagtgg tgtgaaaggc
     2161 acgttcgagt tgta
//
LOCUS       DAOQDO010001093         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47846_length_2172_cov_5.065187, whole genome shotgun sequence.
ACCESSION   DAOQDO010001093 DAOQDO010000000
VERSION     DAOQDO010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47846_length_2172_cov_5.065187"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKZ53_16185"
     CDS             <1..927
                     /locus_tag="PKZ53_16185"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNJ42027.1"
                     /translation="ATNMAASVRQRLMNIFREKGGEFQVLLIRYALERFLFRLSQTVY
                     QPQFCLKGAMMFVFWGGEPHRPTKDLDLLGYGANTISSLESIFREICLCPVVPDGIEF
                     LSETVKGVEIKHNDEYHGVRITLTARLDQALIPLQIDIGFGDLVVPAPQEAFFPTLLE
                     MPAPQLRMYCRETVIAEKFQALVELGMGNTRLKDFYDIWVLIQRYEFEGTQLSQAISA
                     TFTRRMTTLVAEPPLALTGQFALDPTKNSQWKGFLKKNRFSSSLELVQVTTDLWKFFS
                     KPVAALVSGMALSEIWIPADGWKKPQPDRLNG"
     gene            1114..>2172
                     /locus_tag="PKZ53_16190"
     CDS             1114..>2172
                     /locus_tag="PKZ53_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNJ42028.1"
                     /translation="MTYIHEQPEWPNFSWDINLIAPALIQARHHQGRILGRMDSLGFS
                     FQEKANLQILTQDVLNTSAIEGEFLNPEAVRSSIARRLGIEFAGMVTPGRDVEGIVEI
                     MVDATRCYKEPLTPERLFGWHSALFPTGRSGMRKITVGNWRTPDAGPMQVVSGPYGRE
                     QVHFEAPSADRLDSEMDQFLNWFNAPDELDLVVKAGMAHFWFITIHPFEDGNGRIARA
                     IADMVLARADGSPHRFYSMSSQIETERREYYLALERQQRSTTDITRWLLWFLGCLDRA
                     FTKTESFLTTVIFKAHFWEWVNRRPVNQRQQKILNRLLDGFQGQLTSSKYAKITHCST
                     DTALRDIHSLLERGILVQN"
BASE COUNT          549 a          508 c          539 g          576 t
ORIGIN      
        1 gcgaccaata tggcagcttc ggttcggcaa cgattgatga atatttttag ggaaaaaggt
       61 ggagagtttc aggtcttact catccgatat gctctggaac ggtttctgtt tcggctctcc
      121 caaacagtat accaacccca gttttgtctc aaaggtgcca tgatgtttgt tttttgggga
      181 ggtgaacccc atcgcccaac aaaggatttg gatttgttgg gttatggagc gaacacaatc
      241 tcatccctgg aatcaatttt tcgagaaatc tgtctgtgcc cggttgtacc agacgggatt
      301 gagtttttga gtgaaacggt gaagggggtt gaaattaaac acaatgatga gtatcacggg
      361 gtccgcatta cactcacagc caggttggat caggcactga ttccgcttca aatcgatatt
      421 ggttttggag atctggttgt tcccgcacct caagaagcgt tcttcccgac tttgctggaa
      481 atgcccgccc ctcaattgcg catgtattgt cgggaaacag tcatcgctga gaaattccag
      541 gctttggttg aacttggaat gggaaatacc cgactcaaag atttttatga tatctgggtg
      601 ctgatccagc ggtacgaatt tgagggcacc cagttgagcc aggcaatttc agccaccttc
      661 acccgacgaa tgaccacgct ggtggcagaa cctccattgg ccttgaccgg gcagtttgca
      721 cttgacccaa ccaagaattc ccaatggaaa ggctttctca agaaaaaccg gttttcctcc
      781 tcacttgagc tggtacaggt cacaactgac ttgtggaaat ttttttcaaa accagtggcc
      841 gctctggtct caggtatggc cttgagcgaa atctggatac cagccgatgg gtggaagaag
      901 cctcagcctg accggttgaa tgggtaaatc aagtccaatc agcctctatc ctgacgtctt
      961 ccgacaatct cacttcgtcc ctcacctgga aaacaggcac attctctctc aattccctga
     1021 cacagattta cagttgcggt gattaagttc tataatcacc gcatattttg cggcaattat
     1081 agaacttaaa tcactgtgga aagcagagcc accatgactt atatccacga acaacctgaa
     1141 tggccaaatt tctcctggga tatcaatctg atcgcaccag cccttattca ggcaagacac
     1201 caccagggac gaatccttgg acgaatggat tcacttgggt tttcttttca agaaaaagcg
     1261 aaccttcaaa ttttgacgca agacgttctc aacacgtcgg ctatcgaggg agagttcttg
     1321 aatcctgaag ccgttcgatc ctcaattgca cgtcgcttgg ggattgaatt tgctggaatg
     1381 gtgacacctg gtcgggatgt tgaagggatt gttgagatca tggtagatgc tactcggtgt
     1441 tacaaagaac ctttaacacc agaacggctc tttggttggc attcagcact tttcccgaca
     1501 ggccggagtg gaatgcgaaa aatcacggtt ggaaactgga gaacgcccga cgcgggtcca
     1561 atgcaggttg tttctggacc atatggacgt gaacaggttc attttgaagc cccgtccgct
     1621 gaccgccttg acagtgaaat ggatcagttt ttgaactggt tcaacgcacc tgatgagctt
     1681 gacctcgtgg taaaagcggg aatggctcac ttctggttta tcacgattca cccatttgag
     1741 gacgggaatg gtcgaatcgc tcgggcaatt gcggatatgg tgcttgcccg tgccgatggc
     1801 agtcctcatc gattttatag tatgtcatca cagattgaaa ccgaacggcg cgagtattac
     1861 ctcgcactgg agcgtcaaca gcgatccacg acggatataa ctcgctggtt actttggttt
     1921 ctcggttgcc tggatcgggc attcacaaaa accgagagtt ttttaacgac tgtcattttt
     1981 aaagctcatt tttgggagtg ggtgaatcgt cggcctgtga accaacgtca acaaaagata
     2041 ctgaaccggt tgctggacgg gtttcagggg cagttaacca gctcgaaata tgcaaagata
     2101 acgcattgct caaccgacac cgcgttgcgg gatatccact cgctgcttga acgtggcatt
     2161 ttggttcaga at
//
LOCUS       DAOQDO010001094         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_47853_length_2172_cov_3.300897, whole genome shotgun sequence.
ACCESSION   DAOQDO010001094 DAOQDO010000000
VERSION     DAOQDO010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47853_length_2172_cov_3.300897"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKZ53_16195"
     CDS             complement(<1..622)
                     /locus_tag="PKZ53_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ42029.1"
                     /translation="MSRRFITATLLALSVMFFGQACATKKFVRKTIDERVAPLEGRTA
                     ELEESVRRNSSAIKEVDDRLSRRIDTVSVKAEEAEKKATEADRKAVEADRKAVAVRTD
                     LVETSKNIDNFVVVRTAKVYFKTGRFELSPEAKAELDAVASYVKGRKGTRIEIAGYAD
                     RRGGIRYNEKLTENRADEVKRYLFRAHGIDLYRVTYIGAGKIDVAGA"
     gene            complement(879..>2172)
                     /locus_tag="PKZ53_16200"
     CDS             complement(879..>2172)
                     /locus_tag="PKZ53_16200"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNJ42030.1"
                     /translation="KVTYQIEEGKRIFINDIFVTVRGRTHKHVVQQYLLFQKGNLLQQ
                     SKLSQTEQNLYGTGLFNQVVTRIQPVRSDGPYRELYDVFIDVAEGKRYTLSYSVGIEQ
                     QEVQRQATPRFSFEIANRNLFGRLQTASLIFRLSQREQLGQVSYQFPRFLGSQWPFLT
                     TFLFQRSQEVSFSIERKSIQLRTERKLNDLTSLIFRYQFDDVRPFDLEISGDDLDRNN
                     RPVKLGRISSTYLRDSRDSALDATKGSFSIIDLSLASTKIGGTERYLRMFGQYQQFRT
                     LPKIPSVVFASSIRIGLARPYGESINLPISERFFSGGANTIRGLDFEQAGPRDPITDQ
                     PIGGNAVVVLNAELRYPIFRALTGAAFYDTGNVFDKVSNIRLGGFTNTVGTGIRLKTG
                     LGPIRIDMGYNLDPPSFVAVPNQKLSRVQFHISFGQAF"
BASE COUNT          547 a          508 c          581 g          536 t
ORIGIN      
        1 gggcaccggc aacatcaatt ttaccagcgc caatataagt tacacgatac aggtcaattc
       61 cgtgggcccg gaacaggtag cgttttactt catcggcccg gttttcggtc agtttttcat
      121 tgtagcgaat gccgccgcga cggtcggcat agccggcaat ttcaatccgg gtgcctttgc
      181 ggcctttgac atacgaagcg accgcgtcaa gttcagcttt ggcttcaggg ctgagttcaa
      241 accgacctgt tttgaaatag acttttgccg tccgaaccac gacaaagtta tcaatgtttt
      301 tgctggtttc aaccaggtcg gtgcgcacgg caacggcttt gcggtcggct tcaacggctt
      361 tgcggtcagc ttcagttgcc tttttctcag cttcttcggc ttttacgctc acggtatcaa
      421 tccggcggct taaccggtcg tcaacttcct tgatcgcaga tgaattgcga cgtacggatt
      481 cttccaattc agcggtacgc ccttcgagtg gggctacccg ttcatcaatg gttttccgca
      541 cgaacttttt ggtagcacag gcttgtccaa agaacatgac agacaaagcc agcaacgttg
      601 ctgtgataaa acgtcggctc atgggttctc ctcaagtgat agattgaaaa agatgttgtc
      661 ccgcaaatat tgaatgagct tcaaataggg gaattaaaag ggaaaagtgg aatgtgccgt
      721 cttaaatatc agatagagtc agaaagatca atgcttgggg ctgaagaata tcgggctgaa
      781 gaaccagggc tgagggcaat cgaagggaag agggatgaag gataagggat gaactaaaac
      841 caaattttca gcccgacttc ttcagcctca agtcctgctt agaatgcctg cccaaagctg
      901 atgtgaaact ggacgcgtga cagtttctga ttcgggacgg ccacaaagct gggtggatcc
      961 aggttatatc ccatatcaat tcggattggc cccagacctg tttttaggcg gatgcctgtt
     1021 ccaactgtat tggtaaaccc acccagtcgg atgtttgata ccttatcaaa aacattgccg
     1081 gtatcataaa aagcggcacc ggtcagggcc cgaaagatgg ggtagcgcaa ttcggcgttg
     1141 agcaccacca cggcatttcc accaattggc tggtcagtga ttgggtcacg tggtccagcc
     1201 tgttcgaagt caagtccacg gatggtattg gcgccgccag agaaaaatcg ttcgctgata
     1261 ggcaggttga ttgactcgcc gtagggtctg gccagcccga tgcggatact gctggcaaag
     1321 acaactgatg gaattttcgg aagagtgcga aattgctggt actgtccaaa catgcgcagg
     1381 tagcgctcag tgccgccgat tttggtggat gccagcgaca ggtcaataat cgaaaacgac
     1441 cctttggtgg catccagggc ggaatcacgg ctatcccgca gataggtact tgaaatgcgg
     1501 ccaagcttga cggggcgatt gttccgatca aggtcatccc ctgaaatttc caggtcaaat
     1561 gggcggacat catcaaactg atatcggaaa atcaatgagg ttaaatcatt gagtttccgc
     1621 tcagtgcgga gttgaatgga ttttcgctcg atactgaaac tgacttcctg cgagcgctgg
     1681 aacagaaacg tcgtcaaaaa aggccactgt gagcccagaa agcggggaaa ctgatacgaa
     1741 acctggccaa gttgttcacg ctggctcagg cggaaaatca ggctggcagt ttgcagccgg
     1801 ccaaacagat tgcggttggc aatttcaaat gaaaatcggg gtgttgcctg acgctgaacc
     1861 tcctgttgct cgataccgac actgtaagaa agcgtgtagc gttttccctc agcgacatca
     1921 ataaagacat catagagttc acgatagggg ccatcggacc ggactggttg aatgcgagtg
     1981 acaacctggt taaacaggcc cgtgccatac aggttttgtt cagtctggct gagtttgctt
     2041 tgctgcagca ggtttccttt ttgaaacagc aggtactgct gaaccacatg tttatgggtt
     2101 cggccccgca cggttacaaa gatatcgttg ataaaaatgc gtttgccttc ttcgatttga
     2161 taggtgactt tg
//
LOCUS       DAOQDO010001095         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48029_length_2168_cov_2.606720, whole genome shotgun sequence.
ACCESSION   DAOQDO010001095 DAOQDO010000000
VERSION     DAOQDO010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48029_length_2168_cov_2.606720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2108
                     /locus_tag="PKZ53_16205"
     CDS             <1..2108
                     /locus_tag="PKZ53_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42031.1"
                     /translation="IDRAGRLWFGTIGGATFFDPTQEIPDLMAKPLRLESLLINGSSL
                     FRAQGLTQLASEWNTGHEFAHDENNIVIEYSLLSYFREPDSQYCTQLVGLQDRPTPWT
                     TEAKTVYTTLPQGHYRFKVWGRDYAGNISGPVEIGFTIRPAPWVTWWAFVIYGVVVVG
                     GGYSGYQWRVFRFRQANAQLEAKIRERTAALAKSEALTKEKADELARLVTKLSISEQQ
                     AQEAKEEALVQRQQALEASKAKSLFLANMSHELRTPLNAILGFVQLLNRVPGRSDEDR
                     EYLGIIIRSGEHLLNLINNILSLSKIEAGQVVLEEKTFDLRMLLTELEGLFRFRAQTK
                     HLRFAIEFPPELPRFVVGDERKLRQVLINLLGNAFKFTERGEVKLKVSRHQELATFEV
                     SDTGAGIAPAEIGKIFQPFTQTQSGLQAQEGTGLGLTISQDFVNVMGGEITVESQFGR
                     GTTFRFAINLPAVMAGATSIKPKTVIGLATGQPVFRLLVVDDMVENRQLLTTLLKQFG
                     FDVQDAANGAEALTLWQNWRPHLVWMDMHMPVLDGFEATRQIRATESAGKSGASNSIS
                     EVPTWIISISASAFDADRTAILEAGCDDIVIKPFSTDVIVEKLVKYLGVEFTYESSSE
                     ITTETHQVCGQPESLKQAIASLAPELRTQLYEAVLGCNREKVYGVAEHIHSVDPILAQ
                     ELHHLARQYQFEELLTWFD"
BASE COUNT          515 a          534 c          606 g          513 t
ORIGIN      
        1 tgattgaccg ggccgggcgg ctgtggtttg gaacgattgg tggcgccact ttttttgatc
       61 caacccagga aatcccggat ttgatggcca agccactccg gcttgaatca ctgttgatca
      121 acgggtcgtc gttgtttcgg gcacaggggt taactcaact ggccagcgaa tggaacactg
      181 gtcatgaatt tgcccacgac gaaaacaaca ttgtcatcga atacagtttg ctgagttatt
      241 tccgcgaacc ggatagccag tattgtaccc aactggttgg attacaggat cggccaacac
      301 cctggacaac cgaagccaaa accgtgtaca cgacgctgcc gcaaggacac taccggttca
      361 aagtctgggg gcgcgattat gctggaaaca tttctggccc ggtggaaatc ggatttacga
      421 ttcgcccggc gccgtgggta acgtggtggg cctttgtcat ctatggggtt gtggtggttg
      481 gaggtgggta tagcggatat cagtggcggg tgtttcggtt caggcaggcc aacgcccagc
      541 tcgaagccaa aatccgggaa cgaacggcgg cgctggcgaa atcagaagcg ctgaccaaag
      601 aaaaggccga tgaactggcc cgactggtaa ccaaactgag catttcagaa cagcaggctc
      661 aggaggccaa ggaagaagcc ctcgtccagc gtcagcaggc gctggaagcc agcaaagcca
      721 aaagcctgtt tctggccaat atgagtcacg aactccgcac cccgctcaat gccattttgg
      781 gatttgtcca gttgctcaac cgggtaccgg gtcgttcgga cgaagaccgt gaatatctgg
      841 ggattatcat tcgtagcggc gagcatttgc tcaatctgat caataacatt ctctcgctgt
      901 caaaaattga agctggccag gttgttcttg aagaaaaaac ctttgatctg cggatgctct
      961 tgaccgaact cgaaggactc ttccgattcc gagcccagac caaacatctg cggtttgcga
     1021 ttgagtttcc gccagaactc cccaggtttg tggtgggaga tgaacgcaaa ttgcgtcagg
     1081 ttttgatcaa cttgctgggg aatgccttta aatttactga gcgtggcgaa gtgaaattga
     1141 aagtcagccg tcatcaggaa ctggccacct ttgaagtctc agacactggg gccgggattg
     1201 ctccggcaga gattgggaaa attttccagc cctttaccca aacccaaagc gggttgcagg
     1261 cgcaggaagg caccggtttg gggctgacca tcagtcagga ttttgtcaac gtcatgggcg
     1321 gggaaatcac cgtcgagagt cagtttggac gcggaaccac gtttcgtttt gccatcaact
     1381 taccggctgt gatggcgggt gccacctcga tcaagccgaa aaccgtgatt ggtctggcta
     1441 ccgggcaacc tgtttttcgg ctcctggtgg tggatgatat ggtcgaaaac cggcagttac
     1501 tgaccactct gttaaaacag tttgggtttg atgtccagga tgcagccaat ggcgccgagg
     1561 ccctcaccct ctggcaaaac tggcgcccac atctggtgtg gatggatatg catatgccgg
     1621 tcctggacgg atttgaagcc acccgacaga ttcgggccac tgaatcagcc ggaaaatcag
     1681 gtgcttccaa ctcaatttct gaggtaccaa cctggatcat ttcgatttca gccagtgcgt
     1741 ttgacgctga ccggacagct attttagaag ctggttgcga tgacattgtg atcaagccgt
     1801 tttcaacaga tgtcatcgtt gagaaactcg taaaatacct tggagttgag tttacctatg
     1861 agtccagctc tgaaatcacg actgaaacac atcaggtttg tggccagcca gagtccttga
     1921 aacaggccat tgccagtctt gctcctgaat tacgcaccca actgtatgag gcagtacttg
     1981 gctgcaatcg ggaaaaagtt tatggtgtgg cggaacacat tcactcagtt gacccaatcc
     2041 tcgctcagga acttcatcat ctggctcgac agtatcagtt tgaagaacta ttgacctggt
     2101 ttgattgagg aaaaccgggt tccgggttcc gggttccggg ttccgggttc cgggttccgg
     2161 gttccggg
//
LOCUS       DAOQDO010001096         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48053_length_2167_cov_3.303030, whole genome shotgun sequence.
ACCESSION   DAOQDO010001096 DAOQDO010000000
VERSION     DAOQDO010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48053_length_2167_cov_3.303030"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..909
                     /locus_tag="PKZ53_16210"
     CDS             388..909
                     /locus_tag="PKZ53_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNJ42032.1"
                     /translation="MVSEASSVDCPGALNYTVKSIEGKDVSLCSYKGKVVLIVNTASQ
                     CGFTPQYKGLEELYQKYRDRGLRILAFPSNDYGQQEPGTNTEIKQFCEMNYHTTFDLF
                     GKVTVKGDGKAPLFAYLTSGGGNSKLAGEIQWNFSKFLIGPDGKLVARFGSSVTPTST
                     ELTSAIEGLLKAK"
     gene            complement(1073..1582)
                     /locus_tag="PKZ53_16215"
     CDS             complement(1073..1582)
                     /locus_tag="PKZ53_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42033.1"
                     /translation="MCVPAFQADTASQCRLRVMDARELTVQADLISRVRVKKVKEIRD
                     PMYGQLVTLEILEVLKGDTRLKEVTVWAASKVYCAMDIYVKGQEFLVFLTYEQTLYRT
                     LNWQYGQFLMGEPTIKGWRSPGTDPNAIPEKTFEQIKAEIQTFLEDARNPNGEPSPNG
                     NPPALPQRP"
     gene            1864..>2167
                     /locus_tag="PKZ53_16220"
     CDS             1864..>2167
                     /locus_tag="PKZ53_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNJ42034.1"
                     /translation="MSTSPIMKFPALSQVCLQCIACESTFDTAQNIYTCEKCGDLLDV
                     SYDWNSRLVSDYRKLFDARRASANPLDISGVWRFRELLPFYTAESQIVTMFEGNTAL"
BASE COUNT          553 a          508 c          489 g          617 t
ORIGIN      
        1 gacttaaaaa taacctcgcc acctgcacca gatttgatca tgtaacgctc ttttaagaca
       61 aatctccatc catcttcgca tcctggccac ttcttttctc caaggtttac ttgatgttcc
      121 gccatcaatc gtgaatagtc agcctctcaa tcaatcctct tttaccaatc actgttcttt
      181 gttttcaccc acaatattga cctataccga ggttagacct gctgttggaa aactattcca
      241 gttgagtttt atcctggcat caaaattttg actatttttc cctctcagga gacatttccg
      301 atggtacgca aattatcact tgtcatcaca gtcaccttaa tgattggagt aatcagcggg
      361 tgctggtttg gaggaacaca acaacctatg gtttcagaag catccagtgt tgattgtcca
      421 ggtgcgttaa actacaccgt caagtcaatt gaaggcaaag atgtcagtct ttgctcctat
      481 aaaggcaagg tggtgttaat tgtcaacacc gcctcacaat gtggatttac accccaatat
      541 aaaggactcg aagaactgta tcagaagtat cgggatcgtg ggctgcgcat cctggcgttt
      601 ccctcaaacg actatggcca gcaggaacca ggtaccaata ctgagattaa acagttttgc
      661 gagatgaact atcacaccac gtttgattta ttcggcaaag tgacggtcaa aggagatgga
      721 aaggccccgc tgtttgccta tttgacctcg ggtggcggga actcaaaact ggctggagaa
      781 attcagtgga atttttccaa atttctgatt gggccagacg gcaaactggt cgcccgtttt
      841 gggtcaagtg tcactccgac ctcgaccgaa ctgacatcgg cgattgaggg tctgctcaaa
      901 gccaaatgat accattgagg gttcagggta tcgggttcag ggtttagggg aaaacaacaa
      961 atccgagaaa gacgcatttt tctgtgcctt tttctcggat ttggatttta tgaagctgga
     1021 atagcgacca ggcagattca gctcatatca acgatgtgcc gattcatcca ttttatgggc
     1081 gctgtggcaa agcaggaggg tttccatttg gggatggctc accgttgggg tttcgggcat
     1141 cttcaaggaa tgtctgaatt tcagctttga tttgctcaaa ggttttttca ggaatcgcgt
     1201 ttgggtcggt gcccggagag cgccagcctt tgatggttgg ctcacccatc aaaaactgac
     1261 cgtattgcca gttgagggtt cggtagagag tctgttcata ggtgagaaaa accagaaact
     1321 cctggccttt cacatagata tccatggcac agtacacctt gctggctgcc caaacggtga
     1381 cttccttgag tcgagtgtca cctttgagaa cttccagaat ctcaagggtt accagttgac
     1441 catacatcgg atcacggatt tctttgactt tcttgacccg tacccgacta attaaatcag
     1501 cctgtaccgt taattcacgg gcatccatca cccgcaaccg acattgtgaa gcagtgtctg
     1561 cctggaaagc tgggacgcac accccagata ataaaaggat taagcttaaa aacaagattc
     1621 tcatgtgagg ataattgaag cgtgtaaagt aggacttttc aggaaagatt gaattgtgca
     1681 ttgtacctta aatctctaat tttgaaagag tgtcgttcaa agaaatcatt tttgggcttg
     1741 cgcttgggct ggatttctac cacagtttga ttctttgatc agggttggct gttgcacaga
     1801 ctcccggtta aacccaaatc tcctcatttt gttccgaaac cacacctatt ctttttcatg
     1861 gctatgtcta cctcaccgat tatgaaattc ccggcgctca gccaggtttg tttacaatgt
     1921 attgcctgcg aatccacctt tgataccgct caaaatatct acacctgtga aaaatgcggt
     1981 gacttgcttg atgtttccta tgactggaac tcacgcctgg tgtcagacta ccggaagctt
     2041 tttgatgccc gtcgggcatc ggccaaccca ctggatatca gtggagtctg gcgctttcgg
     2101 gagctacttc ccttctatac cgccgagtcc caaattgtca caatgtttga aggcaacacg
     2161 gctctgt
//
LOCUS       DAOQDO010001097         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48060_length_2167_cov_2.817708, whole genome shotgun sequence.
ACCESSION   DAOQDO010001097 DAOQDO010000000
VERSION     DAOQDO010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48060_length_2167_cov_2.817708"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKZ53_16225"
     CDS             complement(<1..906)
                     /locus_tag="PKZ53_16225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42035.1"
                     /translation="MTPLERFQKVQKLFDEVIDRSPDNREAYLRAVCGDDVSLIGEVF
                     ELIRLDQTAQSYFESVESEVQKEMSPAFAGQLLELDQAKGHSGLTGLFHLIGEVLDGK
                     YHIEKQLGTGGMGTVFLARHLHTKRQVAIKVIAPEFAGNKEFLERFRREAAAAGRLRH
                     PNIVNVTDFGIALHQSDQMAYLVMELLEGMTLKTFLKDQNQLSLNLTVDLVEQACLAV
                     SHAHQKGIIHRDLKPENIWLEPNGRGGWNVKVLDFGIAKIREVTPPEAEALPAVISGP
                     QLPADVSLIADEVPTILESPVATLFK"
     gene            complement(1052..1645)
                     /locus_tag="PKZ53_16230"
     CDS             complement(1052..1645)
                     /locus_tag="PKZ53_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNJ42036.1"
                     /translation="MNPGHDSGEVTVLLKQWRQEQNADAYNQLIPLVYNELRRIALAI
                     SFRQGQANPADQTLNPTAIVQQAYLNLIDQHTVEWNDRAHFLAIAAREMRRILVDHWR
                     YKHAQMRGGELQQVSFADIELAQGQPDFDGMALHRALEKLANLDPEEAQIVDLRYFGG
                     LTVDEVAEVLQLSRATIERRERHAKAWIRKQLGSEAL"
BASE COUNT          551 a          515 c          529 g          572 t
ORIGIN      
        1 tttaaacaat gttgcaaccg gagattcaag gattgttgga acttcatcag ctatcagcga
       61 cacgtcagcg ggcaattgag ggcccgagat cacggcgggc aatgcctcag cctctggtgg
      121 tgtcacctcc cgaattttgg cgatgccaaa gtcaagcact ttgacattcc agccgcctct
      181 tccatttggt tctaaccaga tattttcagg ttttaagtca cggtggatga tccctttttg
      241 gtgggcatgg ctgacagcca gacaggcctg ttccaccaga tccaccgtga ggttgagcga
      301 aagctggttc tggtctttga ggaaggtttt taacgtcatt ccttccagca attccatcac
      361 cagataggcc atctggtcgc tctggtgtag ggcgatgcca aagtcggtga cgttgacgat
      421 attggggtgg cgaagtcggc cagccgctgc ggcttcccgt cgaaatcgtt ctaaaaactc
      481 tttattcccg gcaaattcgg gggcaataac cttaatggca acctggcgtt tcgtgtgcag
      541 gtgccgggcg agaaacacgg tgcccatccc accggtacca agctgtttct cgatgtgata
      601 tttcccgtcg aggacttcac caatcaagtg gaacaatccg gttaaaccag aatgaccttt
      661 cgcttgatcc agttcaagga gctgcccggc aaaagccgga gacatttctt tttggacttc
      721 agattcaact gactcaaaat aagattgggc ggtttggtct aagcgaatta actcgaaaac
      781 ctcaccgatg agcgagacat catctccaca aacggctcgt aaataggctt ctcgattatc
      841 tgggcttcga tcaatgactt catcaaagag tttttgaacc ttctgaaaac gttccaatgg
      901 tgtcacaact gtatcctcca cttgactgaa aaatgagctt tcattcaagg agtcgtcaaa
      961 gcgaatcaaa atccatcaaa aaaaatgaaa gtaagcgaaa aaatgaaaga tgacggattt
     1021 agaggttggc agaatcagcc tgagaatcaa gttaaagtgc ttcactccca agttgttttc
     1081 gaatccaggc tttggcgtgc cgttcccggc gttcaattgt ggcgcgactg agttggagca
     1141 cttcggcgac ttcatcaaca gttagtccgc caaagtatcg caaatcaaca atttgagctt
     1201 cttcagggtc caggttggcc agtttttcga gggccctatg cagcgccatt ccatcaaaat
     1261 caggttgccc ttgagccagt tcaatgtctg cgaacgaaac ttgttgaagt tcccctcctc
     1321 gcatttgggc atgtttatag cgccagtgat caaccagaat gcggcgcatt tcccgggcgg
     1381 caatagccag aaaatgggcc cggtcattcc attcgaccgt gtgttgatca atcaaattga
     1441 ggtaggcttg ctggacaatc gcagttggat tgagtgtctg gtctgccgga ttagcctgac
     1501 cctggcgaaa ggagattgcc agtgcaattc ggcgtaactc gttgtaaacc aacggaatca
     1561 actggttata ggcatcagca ttttgttcct gccgccattg cttgagtaag actgtgacct
     1621 cgcccgaatc gtgacctgga ttcatagtat tatttggagt gcgccgactt gtcggcgctt
     1681 tcaattgtgg tgatttgtcg ctcaggaaat aaagccattc agcgacaagt cgctgggaaa
     1741 caaagctctg acaagtcaga gcactccaaa ttgtttttct gaaggcagag actctgaact
     1801 gtatcctgac tcgcaaactg gttgtgtcaa ttgaagttgg aagtgattgg cttgtggagc
     1861 agtttcccgt gaatggtccc cacaatttaa gctgacattg tgccccgatt gacgaaaaag
     1921 cgcaaccaga ttctcaagcg gcacacaagg tggtattcgg aaagcggacc agatttaaat
     1981 gcattgaata aaccaattta aatttaaatc gggaattcaa tcggttgctt ccgcttctgt
     2041 ccttcagtta agaatttgcc agtaccacct gcatcagttg ggctcatttg acaccaactt
     2101 cccggttcag gcagtcggtt gtgagtactt gaatctttct gattgattgc cggtccaaaa
     2161 tccaatc
//
LOCUS       DAOQDO010001098         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48159_length_2165_cov_3.021801, whole genome shotgun sequence.
ACCESSION   DAOQDO010001098 DAOQDO010000000
VERSION     DAOQDO010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48159_length_2165_cov_3.021801"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /gene="dnaG"
                     /locus_tag="PKZ53_16235"
     CDS             <1..1983
                     /gene="dnaG"
                     /locus_tag="PKZ53_16235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNJ42037.1"
                     /translation="HSSFFILHSSFFILNSSFEGCAQMNYPQRVVDEIRSQADIVRVI
                     ADYVALKKRGAAYIACCPFHQEKTPSFNVHPGKQLFKCFGCGVAGNVFSFVMNIEHVP
                     FPEALKIVAQKCGITLPEREFTPQAREAELERERLLELNEWACQFFETQLAKPDGKGA
                     VEYLTARTISPETRALLRLGYAPDRWDALSGYLKSRGATVGEIEKSGLVIVKESGNGH
                     YDRFRGRLIFPIIDTQGRIVAFGGRILGDGEPKYLNSPETVVYTKGNFLYGLSLAKEH
                     IRRLGFVVVVEGYFDFALPFQEGIRNVVATLGTALTENQVRLVRRFMDSPRVIINFDP
                     DQAGQAATRRSIEIFLEHGFKVNILHLPYTDTKYDPDTYVRTFGGAAYRTQLKSSQQF
                     IEYLLDQASIEFDISRPVGKVNVINRVLPYLGRIQEPIERAIYTELFADRLKIDDRLL
                     RDELKKAASGQQTTIATTQRRIEQQESLKPVERRLISSLLAMPELCDWGIQRLAATQI
                     STTPMGEKLLRAIREAHQRSLAIDYDALRAILTQFQREARSPDTLTELPFFDPEAPSD
                     EDPASLLDEQVESLLAELFLEIETPTDEESFQQTFEILENSVLALERQHLEQALAQLQ
                     NQIERSSRAGDHEQAAHFAMQKLELTRQLEKFRRFN"
BASE COUNT          546 a          568 c          529 g          522 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc tcaattcttc attcgaaggg
       61 tgcgcccaaa tgaattatcc gcaacgtgtc gttgatgaaa ttcgctcgca agccgatatt
      121 gttcgggtga ttgcggatta tgtggcgctc aaaaagcgtg gggcggccta tatcgcctgt
      181 tgtccgtttc atcaggaaaa gaccccgagt tttaatgtcc atcctggcaa acaactgttt
      241 aaatgttttg ggtgcggggt tgccgggaat gtgttctcgt ttgtgatgaa tatcgaacac
      301 gttccctttc ctgaagccct caaaattgta gctcaaaaat gcggcatcac gttgccggaa
      361 cgcgagttca cgccccaggc ccgcgaagct gaactggaac gggaacggct ccttgaatta
      421 aatgagtggg cctgccagtt ttttgaaacc cagttggcca aacctgacgg aaaaggtgcg
      481 gttgagtacc tcacggcacg aaccatttcc cccgaaacac gcgctttgct ccggttaggc
      541 tatgcccctg accgatggga tgccctgagt ggatatttaa aaagtcgggg tgccaccgtt
      601 ggtgaaatcg aaaaaagcgg actggtcatc gtcaaagagt cgggaaacgg gcattatgat
      661 cggtttcggg ggcggctgat ttttcccatt attgataccc aggggcgaat tgtggctttt
      721 ggggggcgga tcctgggcga cggcgaaccc aaatacctca attccccgga aacggttgtc
      781 tacaccaagg gcaatttcct ctatgggttg tcactggcga aagaacatat ccgacggctt
      841 gggtttgtgg tggtggtcga aggctatttt gattttgccc tgccgtttca ggaaggcatc
      901 cgcaacgtcg tggcaacgct cggtaccgcc ctcaccgaaa atcaggtccg gctggtccga
      961 cggtttatgg attcgccgcg tgtgattatt aactttgacc cagatcaagc cggccaggca
     1021 gccacccgcc gctcaatcga gatctttctt gagcatggat tcaaggtcaa cattctgcat
     1081 ttgccctata ctgacaccaa atatgacccg gatacctatg tgcgcacctt tggcggggcg
     1141 gcataccgga cccaattaaa aagttcgcag caatttattg aatacttgct cgaccaggcc
     1201 agcattgaat tcgatatttc gcggccagtc ggaaaggtca atgtcatcaa ccgggtgctg
     1261 ccctaccttg gccgaatcca ggaaccgatt gaacgtgcca tttataccga attatttgcc
     1321 gaccggttga aaattgatga ccgtctgctc cgggatgaac ttaaaaaagc agcctccggg
     1381 caacaaacga cgattgccac cacccaacgt cggattgaac agcaagagtc gctcaaacca
     1441 gtcgaacgac ggttgatttc gtcactgctg gcaatgccgg aactgtgcga ttggggaatc
     1501 cagcggctgg cagcaaccca gatttcaacc accccgatgg gtgaaaaatt gctgagagcc
     1561 atccgcgaag cccatcaacg atctcttgcc attgactatg acgcgctacg cgctattctc
     1621 acacaatttc agcgtgaagc acgttcaccc gacacattga ccgaacttcc ctttttcgac
     1681 cctgaagcac cttcagatga agacccggct tcattgctgg acgaacaggt ggaatcactc
     1741 ttagccgaac tttttctcga aatagaaaca ccaaccgacg aagaatcctt tcaacagaca
     1801 tttgaaatcc ttgaaaattc ggttctggcg ttagagcgtc aacatttgga acaggccctg
     1861 gcacagcttc aaaaccagat cgagcgttcc agccgagccg gagaccatga gcaggcagct
     1921 cactttgcca tgcagaagct tgaacttacc cggcaacttg aaaagttcag acggttcaac
     1981 tgatgattgg aaggctgttt gatgcaaccg cctctcaccc aaccaccaac acctgggtac
     2041 ctatcaaaac acgttatgtc cattcacgaa aactatcagg atgacctcga taaactcctt
     2101 gaagggaagc gtaaagatta tctctcatat gatgaactga accagttgct gccgcctgag
     2161 atcaa
//
LOCUS       DAOQDO010001099         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48165_length_2165_cov_2.587678, whole genome shotgun sequence.
ACCESSION   DAOQDO010001099 DAOQDO010000000
VERSION     DAOQDO010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48165_length_2165_cov_2.587678"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PKZ53_16240"
     CDS             <1..1251
                     /locus_tag="PKZ53_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNJ42038.1"
                     /translation="CIAYNRQDGMRNTECNGGLQPAGIKANDGKLWFPTQDGVAVVDP
                     EAVTENRLSPPVLIESVVVEREAVAFQSGITILPGQDDLEISYTGLSFIKSEQIKFKY
                     KLEGKDEDWVDAGTRRTAYYTYIPPGIYTFRVIAANSDGVWNLEGDRIQIRVLAPFWQ
                     KGWFWLICGLSIIGMTVLFFRARVTKLKKQQEVQIAFSRQLIESQEIERKRIAAEMHD
                     SLGQHLLVIKNWAVLGLTLSPSDAPNRSQLDEISETATTALKEVRQIVYDLRPYQLDK
                     IGLTKTLGFMIEQVAESADLLRTSRLDNIDGWFSSDQEIIFYRIVQECLNNIVKHAQA
                     SSVQILIKHERNEVVLTVTDDGCGFEINSRVKSASGFGLTGLIERVQMLGGELTLDSA
                     PGQGTSLTIRFRKKVQAESTSANW"
     gene            1414..2076
                     /locus_tag="PKZ53_16245"
     CDS             1414..2076
                     /locus_tag="PKZ53_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ42039.1"
                     /translation="MSPEIRIVIADDHPVFRRGLQMVITSDPMLKVVGEASGGTEAVS
                     LIQTLHPDVAVLDIDMPDKNGFDVVRRVQTLPSVPKIVFLTMHQDEALFNGAFDLGVQ
                     GYLLKDSAVTDIIKSIKAVAAGEHFISSSLSTFLVNRSRRSVSLLERKPTIEDLTPTE
                     RRVLRLIAENKTSKEIADLLCISLRTVENHRAHICQKLDLRGAHALLKFALTHKQELC
                     SG"
BASE COUNT          535 a          510 c          551 g          569 t
ORIGIN      
        1 tgcattgcct ataaccggca ggacggaatg cgcaataccg agtgcaatgg cgggctgcaa
       61 cctgccggga tcaaggccaa tgatggcaag ctctggttcc caacccagga tggagttgct
      121 gtggttgatc ctgaagccgt gactgagaat cggttgtcgc cgccggttct gattgaatct
      181 gtggttgtgg aacgcgaagc cgttgcgttt cagagcggaa tcactatttt gcctgggcaa
      241 gatgacctgg aaatcagtta caccgggctc agttttatca aatctgaaca gatcaagttc
      301 aaatacaaat tggaaggcaa agacgaagac tgggtggatg ccggtacccg gcgcacggcc
      361 tattacacct acatccctcc agggatttac acctttcgcg tgattgccgc caacagcgat
      421 ggcgtctgga atctggaagg tgaccggatc caaatccggg ttctggcacc gttctggcaa
      481 aaaggatggt tttggttgat atgcggtctg agtataatcg gaatgaccgt gttgttcttc
      541 agggcccgtg ttaccaaatt aaaaaagcag caagaggtcc agattgcttt ctcccgacaa
      601 ttgatcgaat cacaggagat cgagcgcaaa cgaattgcgg ctgaaatgca cgacagcctg
      661 ggccagcatt tacttgtaat caagaattgg gccgtgcttg gactgacgct ttcgccctct
      721 gatgctccga accggagcca actggatgaa atttcggaaa cagccaccac cgctctcaaa
      781 gaagtccgcc agattgtata tgatctgcgt ccctatcagc tcgacaaaat tgggttaacc
      841 aaaaccctcg ggtttatgat cgagcaggtg gctgaatctg ctgaccttct ccgtaccagc
      901 cggttggata acattgatgg ctggttttcc agtgatcagg agatcatttt ttaccgcatt
      961 gtgcaggagt gcctgaacaa cattgtcaaa catgctcaag caagttcagt ccagatattg
     1021 atcaaacacg aaagaaatga ggttgtgctt acagttactg atgatggatg tgggtttgaa
     1081 atcaattcca gggtcaaaag cgcctctggg tttggattga ccgggctgat tgagcgggta
     1141 caaatgttgg gtggtgaact gaccctggac tcagcacccg gtcagggaac aagtctaacc
     1201 attcgatttc gaaagaaggt tcaagctgaa tcaacatcag cgaactggta atactaattt
     1261 gggctgaggg tatcgggcta agggctgaag aaaatattgt atttccctca gctcgaattg
     1321 atgtgatccc agtttgttct tctctcacgc cccaatcatc agtcataatc agtgcctgct
     1381 gatcatcctt tcaccttgtc acgctaaaac attatgtcgc ccgaaattcg cattgtgatt
     1441 gccgacgacc atccggtttt tcgccgtgga ttgcaaatgg tgattacttc tgatccgatg
     1501 ctcaaggtgg tcggagaagc ctcgggcggc actgaagccg tctctctcat ccagacgctc
     1561 catccagacg tggccgtact tgatattgat atgcctgaca aaaacggctt tgatgtggtt
     1621 cgccgtgtgc aaacactgcc gtctgttcca aagattgttt ttctgaccat gcatcaagat
     1681 gaagcgctct ttaatggcgc ctttgatctt ggggtacagg gatatttgct taaagacagt
     1741 gccgtgaccg acattatcaa gagtatcaag gcggtggctg ccggagagca ttttattagt
     1801 tcttcgttat caacctttct ggtcaatcgc agtcggcgca gtgtttcgct gttggagcgc
     1861 aaaccaacga ttgaagatct taccccgact gagcggcgtg tcctccggtt gattgctgaa
     1921 aacaaaactt cgaaagaaat tgccgatctg ttgtgtatca gcctgcgcac cgttgaaaac
     1981 catcgtgccc acatttgtca aaaactcgac ttgcgtggcg cccacgcctt gctcaagttt
     2041 gcgctcactc acaaacaaga attgtgctca gggtaatctc gtttttgggc tcatcccttt
     2101 gcggtgaagg ttttcagtcc gaagttcctg gttgtaatac tattttgcaa tgaatacttt
     2161 atatg
//
LOCUS       DAOQDO010001100         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48166_length_2165_cov_2.570616, whole genome shotgun sequence.
ACCESSION   DAOQDO010001100 DAOQDO010000000
VERSION     DAOQDO010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48166_length_2165_cov_2.570616"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKZ53_16250"
     CDS             <1..554
                     /locus_tag="PKZ53_16250"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNJ42040.1"
                     /translation="QSSPARPPHTVTSFYERVAAKLAANPALAQQIGKPSAELTELQW
                     MTGTWEITARVFATKTTPEQISKGHGVVQSVLNGTWLSFADSYSADAPSDQGFLTYNP
                     ISKKWISLTLDDSGTAGVMTATRWENNRLVFQGPPLELLGETVTLRQTLEKRSNTEYR
                     ILNEEQLPTGKWVPLDEYIYRKK"
     gene            complement(602..>2165)
                     /locus_tag="PKZ53_16255"
     CDS             complement(602..>2165)
                     /locus_tag="PKZ53_16255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42041.1"
                     /translation="NGVIGMTGLLLDTELNPEQREYTETIRKSGEALLTIINDILDFS
                     KIESGRLSLEIIDFDLVTAIEDVFDLLAENAYAKGLECTYSVDADVPTNLCGDPGRLR
                     QILINLIGNAIKFTQAGSVTVNVSLAEGPPPAEKTAEPTTLLRFAVTDTGIGISPNVQ
                     ANLFEVFTQAESSTFRKFGGTGLGLAISKQLVEMMNGTIGLTSEPGRGSTFFFTAQFT
                     IQPEPSGSYARHEATDSGQAYQKVSPIRDYTTTPGISEQRLRVLVAEDSAVNQRVAVK
                     MLEKLGCRADVVANGLEAVNALKLLPYDLVFMDCQMPEMDGFEATSEIRKWELSESTF
                     PDPIPIIAMTANAMQGDRERCLAAGMDDYVAKPVRFESLEAVVQHWRDQIRKAMPAAP
                     PTIKVQMIEGDQPSLDYKVLADLRELSDEGENFLDEVIETFQINASPQIQLLRDSIAH
                     LKENEAERAAHRLKGSCGIVGAKRLAVIAGEIERHARDGHLDRTHRSLELLVDEYQRV
                     CSLLEEEKLKSS"
BASE COUNT          519 a          565 c          544 g          537 t
ORIGIN      
        1 cccagtcatc accagcacgt ccgcctcaca ctgtcacctc tttttatgag cgggtcgccg
       61 caaaattagc cgcaaatcca gcactggctc aacagatagg taaaccatca gccgagctga
      121 ccgagttaca atggatgact ggcacctggg agataacagc gcgggtcttt gcaaccaaaa
      181 ccacgccaga acagatttcg aaagggcacg gagtggtgca gtctgttttg aatggtacct
      241 ggctctcgtt tgctgattca tacagcgccg atgctccatc tgatcaggga ttcctgacct
      301 acaatccaat ctccaaaaaa tggataagtt tgacgctgga tgattctgga acagcagggg
      361 ttatgacggc caccagatgg gaaaacaacc ggctggtttt tcaaggccca ccactcgaat
      421 tacttggcga gacggttacc ctccgccaaa ccctggaaaa acggagcaac acggaatatc
      481 gcatcctgaa tgaagaacaa ctgcccaccg gaaaatgggt tccgttggac gaatatattt
      541 atcggaaaaa atgaccaggt tctttgtctg aaaacatcag cggcttgttg ctcaggttcg
      601 atcatgagct ttttaatttt tcttcttcaa gcagcgaaca tacccgctgg tactcatcta
      661 ccagcaattc aagactgcga tgggtccggt caaggtggcc gtcgcgggcg tggcgttcaa
      721 tttcaccggc aatcacggcc agtcgtttgg cgccgacaat cccacagctt cctttgagac
      781 gatgggctgc ccgttcggcc tcattttcct tcaagtgggc gattgaatcg cgcaacagtt
      841 ggatttgagg gctggcgttg atttgaaagg tttcgatcac ttcatcaaga aagttttcac
      901 cttcatcgct taactcccgc aggtcagcca gcactttgta atcgagtgat ggctggtcac
      961 cctcaatcat ttgtactttg atggttgggg gggccgccgg catagctttg cggatttgat
     1021 ctcgccagtg ttgaaccacg gcttccagtg actcaaagcg gacaggtttg gcgacatagt
     1081 catccattcc ggcagccagg catcgctcgc gatctccctg catggcgttg gctgtcatgg
     1141 caataatcgg aattggatca ggaaacgttg attcagagag ttcccacttt cgaatttcgc
     1201 tggtggcttc aaatccatcc atttcaggca tttgacaatc cataaatacc agatcgtaag
     1261 gcaggagctt gagggcattg acagcttcca gcccgttggc caccacatcc gcccgacaac
     1321 ccagtttttc cagcattttc accgccactc gctgattgac cgcactatct tcggccacca
     1381 gtacccgcaa ccgctgctct gatatacccg gcgttgtcgt gtagtcccgg atgggggaaa
     1441 ctttttgata ggcctggcca gaatctgtcg cttcgtgcct ggcatagctg ccgctgggtt
     1501 ccggctggat ggtaaattga gcggtaaaga aaaacgtgct cccacgacca ggttcactgg
     1561 tcaggccaat ggtgccgttc atcatttcaa ccagttgttt ggaaatggcc agacccagac
     1621 ctgttccacc aaatttgcgg aaggttgatg attctgcctg ggtaaaaacc tcaaataaat
     1681 tggcctgaac gtttggggaa atgccgatgc cggtatcggt gacggcaaat cggagcaagg
     1741 ttgttggctc cgctgttttc tcagcaggag gaggtccttc agccagtgag acgttgacgg
     1801 tcacgcttcc agcctgggta aatttaatcg cattcccaat caaattgatg agaatttggc
     1861 gcagccgacc ggggtctccg cacaggttcg tcgggacatc cgcatccacg ctataagtgc
     1921 attccagccc tttggcatag gcattttctg ccagcaaatc aaagacatct tcaatggcgg
     1981 tcaccagatc aaaatcaatg atttcaagcg atagtctccc agattcaatt tttgaaaaat
     2041 caaggatgtc gttaataatg gtcaacaggg cttcgcccga tttgcggatg gtctcggtat
     2101 attcgcgttg ttcgggattg agttcggtat cgaggagcaa accggtcatg ccaatcaccc
     2161 cgttc
//
LOCUS       DAOQDO010001101         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48332_length_2161_cov_2.357550, whole genome shotgun sequence.
ACCESSION   DAOQDO010001101 DAOQDO010000000
VERSION     DAOQDO010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48332_length_2161_cov_2.357550"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKZ53_16260"
     CDS             complement(<1..306)
                     /locus_tag="PKZ53_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42042.1"
                     /translation="MRSFFSRRSFRRLSSLTVLVGLVLTTVALASAQNQPSENPVQKS
                     RELARKGMQAYQKKDFTAYLENVREAAKLRPNFPPVLYHLARASFLTGNQSESLRLLT
                     "
     gene            complement(389..1036)
                     /locus_tag="PKZ53_16265"
     CDS             complement(389..1036)
                     /locus_tag="PKZ53_16265"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNJ42043.1"
                     /translation="MPQLPDTLCPKLLHFVQHLHQLEPEPLPNQAYGVESFTRLSPNH
                     VQCFSPVLSGVMKFLLIATVCCVSSMVHVWSPQPPALDIQSLAWLGGCWESKQPSLHS
                     QEQWMPPSGGIMIGMGRTVKNGKLVSVEFMELRQDGSNLVFTAHPSGQKTARFQVTSA
                     SATEAIFENPDHDFPQKIEYRLTKPDVLVATVSGNLNGKNRVEQFRFSRVSCEAR"
     gene            1160..1657
                     /locus_tag="PKZ53_16270"
     CDS             1160..1657
                     /locus_tag="PKZ53_16270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ42044.1"
                     /translation="MRITALEEYGLRCLLQLARQPEGDSLSLKEIAKREGISTANAGK
                     VLWLLSNAGIVRSVRGVKGGYVLAKAANEISVGDIIGVLDTEAMDDHCKSFSGAQEVC
                     VHHDDCGIRPVLHTLNQTVHQVLGKITLAHLIHSEGDVNQRLFQLRRIPKTGELEATA
                     FRSSM"
     gene            1708..>2161
                     /locus_tag="PKZ53_16275"
     CDS             1708..>2161
                     /locus_tag="PKZ53_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010996648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNJ42045.1"
                     /translation="MMSTIAALAEQEYKYGFTTEIESEVVPRGLTEDTVRLISEKKNE
                     PAFMLEFRLKAFRQWLKMTPPAWSNVTYPEIDFQDIIYYSAPKPKAKLNSLDEVDPEL
                     LDTFEKLGIPLNEQKMLANVAVDAIFDSVSVATTFREKLAEAGVIFCSF"
BASE COUNT          572 a          499 c          581 g          509 t
ORIGIN      
        1 ggtcagcaat cgcagacttt cagattgatt cccagtcaga aagctggcac gggccagatg
       61 atagagcacg ggcgggaagt ttggccggag tttggcggct tcacgtacat tttccaggta
      121 agccgtaaaa tccttttttt gatacgcctg cattcccttg cgagccagct ctctggactt
      181 ttgaaccggg ttttcagagg gttgattttg agctgaagcc agtgcaactg tcgttaagac
      241 aaggcccacc agcactgtaa gtgaggaaag tcggcgaaat gaacgccgtg agaaaaaaga
      301 tctcatagca gtttgaagtc tgtccttcgt tagaaatttg aggagtgaag tcgtaggcta
      361 aagactcaga tgatggaaca caagtccgtc aacgtgcttc gcaggaaacg cgggaaaacc
      421 gaaactgttc gacccggttt tttccgttca ggtttcccga aacagtggcg acaagaacgt
      481 caggtttcgt taatcggtac tcgatttttt gtgggaagtc gtggtcaggg ttttcaaaaa
      541 tagcttcagt tgctgatgcc gaagttacct gaaagcgagc ggttttttgg cctgatggat
      601 gagccgtaaa aaccagattt gacccatcct gccgcagttc cataaattcg accgagacca
      661 gtttgccatt tttgaccgtt cgacccatgc caatcatgat ccctccggaa ggtggcatcc
      721 actgttcctg gctatgcagg gagggttgtt ttgactccca gcacccacca agccaggcca
      781 gggactggat atctaatgcg ggtggttgcg gtgaccaaac atgcaccatt gaagagacac
      841 aacacacagt ggcgatcaat aaaaatttca taacacctga taaaactgga gaaaagcact
      901 ggacgtgatt gggtgaaagt ctggtaaagg attctacacc ataggcttga ttgggcaatg
      961 gttctggttc aagttggtgc aaatgctgga caaagtggag cagtttggga caaagcgtgt
     1021 caggaagttg tggcaaaacc cagggagtga aagggaacaa tacttgacaa aaaagtaagg
     1081 tattcaatag agtggcactg ctcgccttct gagcagaccc aacttcccca ggtgacacca
     1141 tcacacatcg aatgagttta tgagaattac cgcacttgaa gagtacggcc tccggtgttt
     1201 gctgcaactt gcacgccaac ccgaaggtga ctcgctttca ctcaaggaaa ttgccaaacg
     1261 cgagggcatc tcgacggcca atgccggaaa agtgctctgg ctcctgagca atgccggcat
     1321 tgttcggtca gttcgaggtg tcaagggggg gtatgtgctg gctaaggcgg ccaacgaaat
     1381 ctcggttggc gatattatcg gcgttttgga taccgaagcg atggacgatc attgcaagag
     1441 tttttccggt gcccaggagg tctgtgttca tcacgatgac tgcggcatcc gaccggtact
     1501 ccatacactg aaccagactg tccatcaggt gcttggaaaa attaccctgg cacacctgat
     1561 tcattccgaa ggcgatgtca accagcggtt gtttcagctt cggcgcattc caaaaacggg
     1621 agaactcgaa gccaccgcct tccggtcatc aatgtaggaa ccagaagaac aaaaagtgga
     1681 gcgatcttta gggttagtcg aaggagaatt atgagcacaa ttgcggcact tgcagaacag
     1741 gaatacaaat acgggtttac cactgaaatt gagtcggagg tcgttccgcg cgggctgact
     1801 gaagataccg tccgcctgat ttccgaaaag aagaacgagc cggcatttat gcttgagttt
     1861 cgactcaagg cgtttcgcca gtggctgaaa atgacaccgc cggcctggtc aaacgtcacc
     1921 tatccggaaa ttgattttca ggacatcatt tattactcgg ccccgaaacc caaagccaag
     1981 ctcaacagtc tggatgaagt tgatccggag ttgctcgaca cgtttgaaaa gttgggcatt
     2041 ccgctcaacg aacagaaaat gctggccaat gtggcggttg acgcgatttt cgacagcgtt
     2101 tcggtggcaa cgacctttcg ggaaaaactg gccgaagccg gtgtaatttt ctgctcgttt
     2161 t
//
LOCUS       DAOQDO010001102         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48335_length_2161_cov_2.108737, whole genome shotgun sequence.
ACCESSION   DAOQDO010001102 DAOQDO010000000
VERSION     DAOQDO010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48335_length_2161_cov_2.108737"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKZ53_16280"
     CDS             complement(<1..798)
                     /locus_tag="PKZ53_16280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ42046.1"
                     /translation="MITNKRIVFSHILCPTNLTRESNRALRYAVTLARAYGAKLTVAH
                     WVQGKTELDKATEHRASRIMQEALQDQLLASDYDPLKHEEIILMGDIRASLPMYAASE
                     GVDLVVMRAKVFSPNLIGLVGSTVTAMCRTAPCPILITHAREREWVGKTTNHVDLKRI
                     LVAHDFSGDSDQALEYGLSLAQEFEAEVHVLHVVPQFPQPVPPGDVPYVLNPSQLESK
                     PILDRLARGIPPNVSEWCRISFEIKYGTPYSQILTYARDNEIDLICLG"
     gene            complement(1376..>2161)
                     /locus_tag="PKZ53_16285"
     CDS             complement(1376..>2161)
                     /locus_tag="PKZ53_16285"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNJ42047.1"
                     /translation="LTTYGYTVVFLWVFIEQIGIPVPAMPLLLAAGALAGLGKLDPVT
                     TILAATLAAVVADSIWYELGRRRGAGILKFLCKVSLEPDSCIQSTATSFERYGLKTLL
                     VAKFVPGLNTISQPLAGLMRVPRPKFWVFDWVGTTIWAGTSVGLGMLFHNQLQQLADR
                     VEQFGIWCLIGVATLLVLYLGWKYYQRQDFIRRMRVARITPEILKEKIDTGQLVTVID
                     LRHPRTFHESPQTIPGSLRWTTDDISRRHTEIPRDHEVVLFCA"
BASE COUNT          578 a          547 c          557 g          479 t
ORIGIN      
        1 accaagacaa atcaaatcaa tctcgttgtc acgggcatac gtgagaatct ggctatacgg
       61 cgtaccatat ttgatttcaa acgagatgcg gcaccactca ctgacatttg gcggaatccc
      121 tcgggccagc cgatccaaaa ttggcttgga ttcaagctgt gaaggattca aaacgtatgg
      181 aacatcaccg ggtggaactg gttgaggaaa ctgaggcacc acatgcaaga catgcacttc
      241 ggcttcaaat tcctgggcca gcgataaccc atattccagt gcctggtctg aatcacccga
      301 gaaatcatgg gcgaccagaa tccgttttaa atcaacatga ttggtagttt tgccaaccca
      361 ttcccgttca cgggcgtgag taattaagat gggacagggg gcagttcggc acatggcggt
      421 gaccgttgaa ccaaccagtc cgatcaggtt gggcgaaaag accttcgccc gcatcacaac
      481 cagatcaacc ccctcggaag cggcatacat tggtaacgaa gcgcgaatat cacccatcag
      541 gataatctct tcgtgcttga gcgggtcgta atcagacgcc agcagttgat cctgcagcgc
      601 ctcttgcata attcgagaag cccggtgctc agtggcttta tcaagttcgg ttttaccctg
      661 aacccagtgg gcaacggtca gtttggcccc ataagcccga gccagtgtaa ccgcatagcg
      721 gagtgcccgg tttgattccc gggtcaaatt tgttggacag aggatatgag aaaagacgat
      781 ccgtttattg gtgatcatac tttttgtttc gcgcgaggta acaaatgtcg cggcctcctt
      841 tccagcaact attgcgtgca aaaaagatgc ctaaactaaa aaactcccgg taaagcagtt
      901 taaccagaga gtttaaagtg aattagattg ttagaaagca ataagtaacc cttttgagta
      961 caatttttaa caaatagaaa cacaaaaaat gttcacgtag aaaacatatg taaccactga
     1021 acgagtatca gggtggatga caaagccgaa ttgggaaatt ccactatcag ttgaggaaaa
     1081 atacacggga atagggctga agaaattggg cttggggctg agggctgaag aagacggact
     1141 gaagacaacg ggctcaggga ttaaacccgt tttcttccgc cccgagcttg cgagtcttca
     1201 gcccctagcc ctcagcccct agcccgactt cttcagccct ggtttttcaa gaaatttcct
     1261 caattgggaa atttcgttcc agccagccgt caaagccgcc ttccagcggt ctgacgttgg
     1321 taatgccatg tttgtggagg atgagcgccg cacgggcgct gcttttttcg ttcggtcaag
     1381 cacaaaacag gaccacctca tggtcccgtg gaatttcagt atgccgtcgg ctgatgtcgt
     1441 cggtcgtcca gcggagcgat ccgggtatgg tttgcggaga ctcgtgaaag gtgcgtggat
     1501 gacgcaaatc aatcacggtg accagttgac cagtgtcaat tttttctttg aggatttcag
     1561 gcgtaatgcg cgccacccgc atccggcgaa tgaagtcctg ccgctggtaa tatttccatc
     1621 caaggtagag gaccagcagg gttgcgacgc caatcagaca ccaaattccg aactgctcaa
     1681 ctcggtcagc cagttgctga agctgattgt gaaacaacat gccaagcccg accgaagtcc
     1741 cggcccaaat cgtcgtgcca acccaatcaa acacccagaa tttcggacgt gggacgcgca
     1801 tcagtccagc cagcggctga gagatggtat tgagaccagg gacaaacttg gccaccagca
     1861 gggttttcaa gccatagcgt tcaaaactgg tagccgtgga ttgaatacag gaatctggtt
     1921 cgagtgacac tttacacagg aatttgagaa ttccagcccc gcgacggcgt ccaagttcat
     1981 accagattga atcggccacc accgccgcca gcgtggcggc caggatggtc gtcaccgggt
     2041 caagtttgcc gagtccagcc agcgctccgg cagccaggag caggggcatg gctggaactg
     2101 gaattccaat ttgctcgata aacacccata gaaacaccac ggtgtagcca taggttgtta
     2161 a
//
LOCUS       DAOQDO010001103         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48361_length_2160_cov_3.386698, whole genome shotgun sequence.
ACCESSION   DAOQDO010001103 DAOQDO010000000
VERSION     DAOQDO010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48361_length_2160_cov_3.386698"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKZ53_16290"
     CDS             complement(<1..463)
                     /locus_tag="PKZ53_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNJ42048.1"
                     /translation="MPVTETEPVVSPSTPAPAPKPKPESKKLPPPKPVYHPVRKAIHA
                     ICVVIFVILPFFNVMRFDIPRQRFYIIGQELWINEFGIIFFSLMFLMFLIAGTSMIYG
                     RVYCGYMCPQMIFSEAATEFELAIKRWTNKKFPKLDAKKRNWFNTGIFYTVV"
     gene            complement(649..>2160)
                     /locus_tag="PKZ53_16295"
     CDS             complement(649..>2160)
                     /locus_tag="PKZ53_16295"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ42049.1"
                     /translation="TRIGYKVTVFEANAEPGGMLMVGVPVLRLPRELVRHEINAILSM
                     GVELKCNMRLGRDFTIQSLRAEGYKAIFLGIGLPKGRKIPLPGSDVDMVYDGMDFLRA
                     FNEGKPLPLGKKVVVIGGGNVAYDVARSAIRPVMTSDDDSDEFSIEGEREQRIAYDVA
                     RSALRMSGDKEVHIVCLESRVEMPADEIEIHEGSEEGIKLHNSRGPKEILSKKGKVTG
                     LRTVKCTAVFDAQGRFSPTFDENEIEDIEADTVLFAIGQTSDLSFLSPEDGIESVRGL
                     IKVNPDTYQTTAPDVFACGDIAHGPRLFIHAVASAHTASRSMHDYLRKTRTDVVVRKR
                     WFPASYTMVDGWYQLERHNPPAVEPEKRASSLINTEENYSETEAQTQAARCLRCNVNT
                     VFDTTMCIACRGCVDVCPENLIKLVGLSQLPKDPKLMNLAEEGLGITEQDLLSYSTEE
                     LDQMGSVMLKDETTCIRCAMCASRCPTHAITMQRFEFYRECVTIPAPNPKIRY"
BASE COUNT          513 a          582 c          538 g          527 t
ORIGIN      
        1 caaccactgt gtaaaaaata ccagtgttga accagtttcg cttctttgca tccagtttgg
       61 ggaatttttt attggtccag cgcttgatgg ccagttcaaa ctcagtggcg gcttcgctaa
      121 agatcatctg cgggcacata tagccacaat agacccggcc ataaatcatt gaagtcccgg
      181 caatcaagaa catcaaaaac atcaggctga aaaagataat cccaaactcg ttgatccaga
      241 gttcctgccc gatgatataa aaccgctgcc ggggaatatc aaaccgcatc acgttaaaaa
      301 acggaaggat gacaaaaatc accacgcaga tggcatgaat cgctttgcgc accgggtgat
      361 acaccggctt tggaggcgga agttttttgg attcgggttt gggttttgga gcaggagcgg
      421 gagttgaagg tgatacgact ggttcggttt cagttacagg catgattttt tccccaccac
      481 agtgtgaatt tctacccaac cagtttttag ggcttggggc ttagggctgg gggctggggg
      541 ctgaagactc gcaagctcag ggctgaagaa actgggttta ataccctcag cccttagccc
      601 ccagccccca gcccaatgtc ttcagcccga tgtcttcagc cccaagccct aataccgaat
      661 cttcgggttt ggcgcgggga tggtcacgca ttcacggtag aattcaaatc gctgcatggt
      721 gatggcgtgg gtggggcatc gagaggcaca catcgcgcac cggatacagg ttgtttcatc
      781 cttgagcata accgacccca tctggtcgag ttcttctgtg ctgtatgaaa gcaaatcctg
      841 ttccgtaatt cccaatcctt cttcagccag gttcatcaat ttcggatctt ttggaagctg
      901 agaaagtcca accagtttaa tcaggttctc agggcagaca tcaacgcagc cacggcaggc
      961 aatacacatc gtggtgtcaa ataccgtgtt gacattacag cgcaaacacc gggcagcctg
     1021 ggtctgggct tcggtctcag aatagttttc ctcggtgttg atgagtgaac tggcacgctt
     1081 ttcaggttcg accgctggtg ggttgtggcg ttccagttga taccagccat ccaccatcgt
     1141 gtaggaagcc gggaaccacc gtttgcgcac gacaacatcc gtccgggtct ttctcagata
     1201 atcgtgcatc gagcgggacg ccgtatgcgc cgaagccacg gcatggataa acaaccgtgg
     1261 accgtgcgcg atgtcgccac aggcaaacac gtcaggcgcc gtggtttgat aggtgtccgg
     1321 attgactttg atcaggccgc gcaccgattc aataccatct tcgggtgaca gaaatgacaa
     1381 atccgacgtc tggccaatcg caaataaaac ggtatcggct tcaatgtcct caatctcatt
     1441 ttcatcaaat gtcggcgaaa atcggccctg ggcatcaaaa acggcggtac atttgacggt
     1501 tcgcaggcca gtaaccttcc cttttttgct caatatctct tttggaccac gtgagttgtg
     1561 gagtttgatg ccttcttctg agccttcgtg gatttcaatt tcatcggctg gcatttcaac
     1621 ccggctttca agacagacaa tgtggacttc tttatcgccg ctcatcctca gcgccgagcg
     1681 ggccacgtca taggcaatgc gttgctcgcg ttcgccttca attgaaaact catcgctgtc
     1741 atcatcgctg gtcatcactg gccgaatggc cgagcgggcc acgtcatagg ccacgttgcc
     1801 gccaccaatc acaaccactt tcttgcctag cggcaatggt ttgccttcgt taaacgcccg
     1861 caaaaagtcc atgccgtcat acaccatgtc aacgtcagag cccggaagcg ggatttttcg
     1921 ccctttcgga agcccaatcc caaggaatat ggctttatat ccttcggccc gcaggctttg
     1981 aatcgtgaaa tcacgtccca gccgcatgtt gcatttcagt tccacgccca ttgataaaat
     2041 ggcgttaatt tcgtgtcgaa ccagttcacg cggcagccgt aaaaccggca ccccaaccat
     2101 cagcattccg cccggctcgg cgttggcttc aaaaacagtg actttatagc caatccgggt
//
LOCUS       DAOQDO010001104         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48440_length_2158_cov_3.734189, whole genome shotgun sequence.
ACCESSION   DAOQDO010001104 DAOQDO010000000
VERSION     DAOQDO010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48440_length_2158_cov_3.734189"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..665)
                     /locus_tag="PKZ53_16300"
     CDS             complement(111..665)
                     /locus_tag="PKZ53_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42050.1"
                     /translation="MASFAVNQVHLARRCEICHQSDVFDPQTETCARCARISLKNQLL
                     SVYEPAQTPSKIVLARRAQEAKLKDRAFSTAKEAKFGSHIFSPDQPLRFGFVVTGAMH
                     WAGLMFWVAGLALFGSGQSARVLTFLIGLVLCLGLPMVMPFVIARKHGWSWLSGHQIL
                     LTVIGSTLSLLTWVLIYVTLGRMH"
     gene            complement(688..1743)
                     /locus_tag="PKZ53_16305"
     CDS             complement(688..1743)
                     /locus_tag="PKZ53_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ42051.1"
                     /translation="MIVRCALLCVLMIWGGLSDPSVLAQSGRKPGSPPSSNTLPFPNA
                     NPSQDDVISRVRTNEIVLPVTVRNDYGRLSTDLSARDFIIVEDGTRQEITSFNTQRIP
                     LKVVLLLDVSGSVFSEMEAIRKASIEFVRQLSPDDEVAVIQFGKSVELLQNWTSDRAK
                     IERAINWEYRATQANFTETHLWDGMFLAADELLPKVEGRRTILLLTDCDDNGSRVTDA
                     QALGAVVRSNSSLYVISKARAIAEDIKQKYGGKRGAIAGTRSQANALYSRFIEDEARM
                     SQLSERSGGKLFNAMTSEDLTTAYRQVCEELQSQYVLTYISSNETPKSTFRKISVIVT
                     RPGMKASWREGYYLETN"
     gene            complement(1904..>2158)
                     /locus_tag="PKZ53_16310"
     CDS             complement(1904..>2158)
                     /locus_tag="PKZ53_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42052.1"
                     /translation="AIGNLRDFDWDFGALWASQAMADRRKHVKNGCYCTHESNCYYPS
                     LAFNPGHLVKIKRLERNIRDSFREAEAREKATGQVAVESR"
BASE COUNT          537 a          544 c          558 g          519 t
ORIGIN      
        1 aaaagtgagt cgctgaagga aaccattgga agaattgggt ttattttctg tgtgttctat
       61 gtattccgtg gtttctgtat ctgaaattcg atagatgagt ggcagcccgg ttagtgcatc
      121 cttcccaaag tgacataaat caacacccag gtcagtaaac tgagggttga tccaatgaca
      181 gtcagcagta tttgatggcc ggaaagccag gaccagccgt gttttctggc aatcacaaat
      241 ggcataacca ttggaagtcc caaacacaaa acaagcccga tcaaaaaggt cagcactctg
      301 gctgattggc ctgatccaaa aagcgcaagc ccggccaccc aaaacatcaa tcctgcccag
      361 tgcatcgcac ccgtgacaac aaatccaaac cggagaggct gatctggaga aaatatatga
      421 ctcccaaatt ttgcctcttt ggcagttgag aacgcacgat ccttcaattt ggcttcttgc
      481 gctcttctcg ccaggacaat tttggaaggg gtttgtgctg gttcatatac cgagagaagc
      541 tggtttttga gagagatcct ggcacagcgg gcacaggttt cagtttgtgg atcaaagaca
      601 tccgattgat gacagatttc acaccgccgg gctaaatgaa cctgattgac ggcaaaagat
      661 gccataaccc cctcgcgctt ccttccgcta attcgtttcc aaatagtagc cctcccgcca
      721 actggccttc atccccggac gcgtcacgat gacggagatt ttgcgaaaag ttgactttgg
      781 ggtttcattg cttgaaatgt aggtcaaaac atactgactt tgaagttctt cacagacctg
      841 acgataggct gtcgtcaggt cttcgcttgt catggcatta aataatttgc cgccagatcg
      901 ttcggaaagc tgcgacatcc gggcttcatc ttcgataaag cgactgtaca gggcattggc
      961 ctgacttcgt gttccagcaa tcgcaccgcg tttcccacca tatttctgct tgatgtcttc
     1021 ggcaatcgca cgggctttgc tgatgacata gaggctggag ttagatcgca cgacggcgcc
     1081 aagcgcctga gcatccgtga ctcgactgcc attgtcgtca caatcggtga gcagcaggat
     1141 cgtgcgacgg ccttcaactt ttggaagcag ttcgtctgcc gccagaaaca tgccgtccca
     1201 gagatgggtt tcggtgaaat tcgcctgggt cgcccggtac tcccagttga tggcacgttc
     1261 gattttggcc cggtccgatg tccagttctg aagtagctca actgattttc caaattgaat
     1321 cacagcaact tcgtcatcag gtgaaagctg gcgcacaaac tcaatcgaag ccttacggat
     1381 ggcttccatc tccgagaaga cgctgcctga aacatcgagc aggagcacga ccttgagtgg
     1441 aatgcgttgc gtgttgaaac tggtgatttc ctggcgggtg ccatcttcga cgatgatgaa
     1501 atcacgagcg ctcaaatcag ttgaaagccg accataatcg ttgcggaccg ttaccgggag
     1561 cacgatttcg ttggttcgaa cccgactgat gacatcatct tgcgaaggat tggcgttggg
     1621 gaaggggagg gtgttggagg aaggtggaga ccctggtttg cgtccgctct gtgccagaac
     1681 cgaaggatct gacaaccccc cccaaatcat cagcacacag agcaaagcac atcgaacaat
     1741 cataaaaact gcctgagatg acgaaaggct gaagatctcg ggctgaaaaa cctgagctga
     1801 agaaaatggg ctgaagaaaa tgggccggag acgaagatac ccgttttctt cagctctgag
     1861 cttgcgaatc ttcagcccca agcccgtttt cttcagctcg agtttagcga ctttcaaccg
     1921 caacctgacc cgtggctttt tcacgggctt cggcttcgcg gaatgaatcg cggatattgc
     1981 gttcaagccg cttgatcttc accagatggc ctggattaaa ggccagcgat gggtaatagc
     2041 aattgctttc gtgggtgcag tagcagccgt ttttgacgtg tttgcgtcgg tcagccatgg
     2101 cctgtgatgc ccacagcgcg ccaaaatccc agtcaaaatc gcggaggttg ccgatggc
//
LOCUS       DAOQDO010001105         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48443_length_2158_cov_3.307180, whole genome shotgun sequence.
ACCESSION   DAOQDO010001105 DAOQDO010000000
VERSION     DAOQDO010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48443_length_2158_cov_3.307180"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1170)
                     /locus_tag="PKZ53_16315"
     CDS             complement(193..1170)
                     /locus_tag="PKZ53_16315"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNJ42053.1"
                     /translation="MTTLKPRLVFSEHYDIRLFGMEKLHPFDSCKYGRVWDELKSIFG
                     NQLEGITHSVDREVSREELLLVHTHDYLHELESSIYIARALELPFVSAIPAMVLDSKI
                     LKSMRYAVRGTILAAQEALTHGIGVNLAGGYHHASRGRGEGFCIYADIPIAIAELHRT
                     KELSPEDKILILDLDAHQGNGIERICNQLSFQNVFIFDVYNEDIYPGDTEAKKRINFN
                     FPIQTNTADGVYTGIVMEYLPRVLKELGNVKLVFYNAGTDIFEHDPLGQLKVTENGVL
                     SRDTFVFNTLMTANLPFVMVLSGGYTKQSYHLVSRSLRYLLERSGMEPK"
     gene            complement(1176..>2158)
                     /locus_tag="PKZ53_16320"
     CDS             complement(1176..>2158)
                     /locus_tag="PKZ53_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ42054.1"
                     /translation="TVPLVSFWVLHLVWIFPFSVFLPLAARNLPEMVFSTRRSEQLRL
                     FAWIWFLVVVGFFSFSTRQEYYTFPCFPALALLAGTGLAKGESEHSKWVLGGQTFLAL
                     LGVVIASVLGYFLWLSWGVPAASDISKVLTSNPENYRLALGHMSDLTVEAFAVLRNPA
                     LGAALTLGIGFSLAFGLRLLRKHLGATVATAITVAVFLYFAHSALGFFDPYLSSKQLA
                     MTIKQQMKPGDVVVINGEYQGGSSIGFYLPEKVLLLNGRMTGLEFGSHYPDAPKVFLE
                     TPDFVTLWQSEKRVFLFTHDATFDALKAKLPREPVRIAAAGEKSVYCNRP"
BASE COUNT          561 a          566 c          551 g          480 t
ORIGIN      
        1 ccaggaggga tgtcgcggcg gtggttcggg aattagcgac ccaaccagtg aaactgatcc
       61 ccgttgctgc cttccagcgg ggttggtacc ccaaatttga tctcaaagaa tatgctgccg
      121 cctaaaggcg ggactcttga cactctcttt atttccaaac taagagtggt tgctcctgac
      181 tccaaactga tattatttcg gctccatgcc acttcgctcc agaagatagc gtaaacttcg
      241 tgacaccaga tggtagcttt gtttggtata gccgccgctc aacaccatta caaagggtaa
      301 attcgcggtc atcaacgtgt taaacacaaa cgtatcgcgg ctcagaacac cattttctgt
      361 tactttgagt tgcccgagcg ggtcatgttc aaaaatgtcg gttccagcat tataaaaaac
      421 cagtttgaca ttgcccagtt ccttgagcac acgcggcagg tactccatca caatcccggt
      481 atagacacca tcggctgtat tggtttggat tggaaagtta aaattgattc gctttttggc
      541 ttcggtgtct ccagggtaga tgtcttcgtt atagacatca aagatgaaca cattttgaaa
      601 gctcaactgg ttacaaatgc gctcaatccc gttgccttgg tgagcatcca ggtcgagaat
      661 caggatttta tcctccgggc tcaactcttt ggtgcggtgc agttcagcaa tcgcaatcgg
      721 aatatcggcg taaatgcaaa aaccctctcc ccgacctcga cttgcatggt gatagccccc
      781 ggccagattg accccgattc cgtgcgtcag ggcttcctga gcggccagaa tcgtgccccg
      841 aacggcatac cgcatggatt tgagaatttt cgaatccaag accatcgccg gaatggccga
      901 cacaaacggc aattccagcg cccgggcaat gtagattgag ctttccagtt cgtgcagata
      961 gtcgtgggtg tgaaccagca ggagttcttc acggctgact tcccgatcaa ccgaatgggt
     1021 aatgccttca agttgattcc caaaaatgga tttgagttca tcccagacac gtccatattt
     1081 gcacgaatca aatggatgca gcttttccat cccaaacaag cggatgtcat aatgttcgga
     1141 aaagaccagc cgtggcttca aggtagtcat ttggttcaag gccgattgca atagacagat
     1201 ttttcgcctg cggcggcgat tcgaacaggt tcgcgtggga gtttggcttt gagggcgtca
     1261 aatgtagcat catgcgtgaa aagaaacacg cgtttttcac tctgccagag ggtcacaaaa
     1321 tcaggcgttt ccagaaaaac cttgggggca tcaggatagt gagaaccaaa ttccagtccg
     1381 gtcatccgtc cattgagcaa gagcactttt tccggcagat aaaagccgat gctggagccg
     1441 ccctgatatt cgccgttgat caccacgaca tcaccaggtt tcatctgctg tttgatcgtc
     1501 atagccagtt gtttggatga caaatatggg tcgaaaaagc ccagcgccga gtgggcaaag
     1561 tacaaaaaga cggccacggt gattgccgtg gccacggttg cccccaggtg tttgcgcagc
     1621 aaccgcaacc caaacgccag tgaaaaccca atccccaggg tcaaggcggc gccaagcgcc
     1681 ggatttcgca gcacggcaaa ggcttcaacg gtcaaatctg acatatgccc aagtgccagc
     1741 cggtaatttt cagggttgct cgtcagcact ttggaaatat cgctggcggc tggaacaccc
     1801 cacgacaacc agagaaaata gccaagcaca ctggcaatca cgacaccgag cagggccaga
     1861 aacgtttgtc cgccgagcac ccacttgctg tgctcagatt cgcctttggc cagtcccgtg
     1921 ccagccagca aggccagcgc cggaaagcag gggaacgtgt agtattcttg ccgggtggaa
     1981 aatgagaaaa agcccaccac caccaggaac cagatccagg caaagaggcg cagttgttcg
     2041 ctccgacggg ttgaaaagac catctccggc aggttgcggg ctgcaagcgg caaaaagacg
     2101 ctaaatggga agatccagac cagatgcagc acccagaagg aaaccagtgg tacggtgt
//
LOCUS       DAOQDO010001106         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48502_length_2157_cov_3.196955, whole genome shotgun sequence.
ACCESSION   DAOQDO010001106 DAOQDO010000000
VERSION     DAOQDO010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48502_length_2157_cov_3.196955"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="PKZ53_16325"
     CDS             complement(<1..1882)
                     /locus_tag="PKZ53_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ42055.1"
                     /translation="MTTPVRRFVTTTLTGLTLGLCLANPISTSVIFAQQKTVITRADE
                     LPRRSLQVQGKALELLKDTAQLTRLADILIASLNDDLKKYDIQDKATLTGYYQGLIAL
                     HFFKGEFDQTISLIPKLRELENKPADKLLAGSFIEAYIKAAKSTGTTEGAAFKQAFEK
                     NYAEAYARLPFAEIKNEIESGLGSLRMSNPELILGSVESQLQPLLDNTKGNVPESVPL
                     GLIGLRMSMDRRIPLKDEMVRVFENLLASNQASAVTTSDIWESRKATLPATASLSPVI
                     AAVWDSGVDMKVLPEANRFVNPKEVVDGKDNDGNGYVDDVNGIGYDLISATKSIGTLD
                     NPVGKIKSDVKELQKLTKGSLDLQSAIQSPEAQALQKTVAGLKREQATSFQEELSFYG
                     GFAHGTHVAGIVADGNPAIKVLGARMTWDYRTLPNAPSRQRSEFTAQMYRDTVAYFKR
                     NGVKVVNMSWRYNSSAYEGLLTLHGIGKDEQDRKRIANELFKIERDALYDAIRQAGDI
                     LFVCGSGNENNDANFSEYIPASFDLPNLITIGAVDSEGKRTSFTTEGRSVDFYANGFE
                     VESFVPGGDRLKFSGTSMASPQVANLAAKLLAINPKLKPAELVQLIAQGAESSPESAQ
                     IKL"
     gene            complement(1967..>2157)
                     /locus_tag="PKZ53_16330"
     CDS             complement(1967..>2157)
                     /locus_tag="PKZ53_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ42056.1"
                     /translation="VLELRLEKARQILHNRRNDVPLARLALDCGFNEVSSFTKAYKRR
                     FGVLPSQDRPGSRTQLDV"
BASE COUNT          485 a          540 c          552 g          580 t
ORIGIN      
        1 tcaatttgat ctgtgcgctt tcgggtgagc tttcggctcc ctgggcaatc aattgcacca
       61 gttcggctgg tttgagtttg ggattgatgg ccagcaactt ggcagccagg ttggcaacct
      121 gtggtgacgc catcgaagta ccagagaatt tcaaccggtc accgcctgga acaaagcttt
      181 cgacttcaaa tccattggca tagaaatcaa cgcttcggcc ttcagtggta aatgaggttc
      241 gttttccttc gctatccacg gcgccaatcg tgatcaggtt aggcagatca aacgaagccg
      301 gaatatattc tgaaaagttg gcatcgttgt tttcattgcc tgaaccgcac acaaacagaa
      361 tatcacctgc ctggcggatg gcgtcataga gggcatcacg ttcgatcttg aataattcgt
      421 tggcaattcg cttgcggtcc tgctcgtctt tcccaatgcc gtgaagggtc aacagacctt
      481 cataggcact gctgttataa cgccagctca tattgacgac tttgacacca ttgcgtttga
      541 aataggccac cgtgtcacgg tacatctggg cggtaaattc tgaacgctgc cggctcggtg
      601 cattgggcaa tgtccggtag tcccaggtca ttcgcgctcc gagcaccttg atggctggat
      661 ttccgtccgc cacgattccc gccacgtgcg ttccgtgtgc aaaaccacca tagaaactca
      721 attcttcttg aaaagacgtg gcctgctcgc gtttgagtcc ggccaccgtt ttctgcaggg
      781 cctgagcttc ggggctctga atcgctgact gcaaatcaag cgagcctttg gtcagttttt
      841 gcaattcttt cacatcgctt ttgatttttc cgaccgggtt atcaagtgtg ccgatgcttt
      901 tggtggctga aatcagatca tagccaatgc cattgacatc atccacataa ccgttgccat
      961 cattatcttt tccatccaca acttctttcg ggttcacaaa gcggttggcc tcaggcagga
     1021 ccttcatatc cactcctgag tcccaaacgg cggcaatcac tggagacaaa ctggcagtgg
     1081 ccggtaaggt ggcttttcga gactcccaga tgtcacttgt tgtgacggct gatgcctgat
     1141 ttgaagccag aaggttttca aaaacccgta ccatctcatc tttgagcggg attcggcgat
     1201 ccatactcat tctgagccca atcaggccga ggggaacgga ttccggaaca ttgcctttgg
     1261 tgttatcgag cagaggttgg agttgtgact caaccgaccc gaggatgagt tccgggtttg
     1321 acattcgaag gcttccaagc ccagattcaa tctcattttt gatttcggca aagggaagcc
     1381 gggcataggc ttccgcataa tttttttcaa atgcctgttt gaacgccgcg ccttcagtcg
     1441 tgccggttga ctttgcggct ttgatataag cttcaatgaa actcccagcc agaagtttat
     1501 cagcgggctt gttttccaat tcccgtaatt ttggaatgag actgatggtc tgatcaaact
     1561 cccctttgaa aaaatgcaat gcaatcaatc cctgataata gccagtcagt gtggctttgt
     1621 cttgaatgtc atatttcttt aaatcgtcat tcaagctggc aatcaggatg tctgccagac
     1681 gggtgagttg agcggtgtcc ttgagcagtt caagcgcctt cccctggacc tggagtgatc
     1741 ggcggggaag ttcatcggcc ctggtaatca ccgttttctg ttgggcgaaa atgactgaag
     1801 tcgaaattgg attggcgaga caaaggccca gcgtgagccc ggtgagtgtc gttgtgacga
     1861 atcggcgaac tggggtggtc atggatgttt ctccgtgttg gtgtgatcgc ttggtatcac
     1921 acggtacggc ccccaaaatg gcccgccttg aaaaaattgc taattttcag acatcaagct
     1981 gcgtcctgga acctgggcga tcctgactgg gcaagactcc aaaccggcgt ttataggctt
     2041 tggtaaagct gctcacctcg ttaaacccac aatcgagcgc taatcgggca aggggaacgt
     2101 cattgcgacg gttgtgcaat atctggcggg ctttttcaag ccgcagttca agcacgt
//
LOCUS       DAOQDO010001107         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48517_length_2157_cov_2.584206, whole genome shotgun sequence.
ACCESSION   DAOQDO010001107 DAOQDO010000000
VERSION     DAOQDO010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48517_length_2157_cov_2.584206"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKZ53_16335"
     CDS             <1..591
                     /locus_tag="PKZ53_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42057.1"
                     /translation="GRNAIATELEGLKASVLTLANRQNPDGAYFFGGTRQTAPPFDPV
                     TATPAGTPTSPTLIQIEPQANPIQAGVTAESVFINGPDTVFSTLDELVTALRGTGNPV
                     ADKSTLQAGLGKVTTFAQQAISARTVIGKGLNSVETAQGYLDQYSFSLDETLTQVAGA
                     DFAESALQLTQANQALEATLQSGASIGRKSLLDFLG"
     gene            734..1411
                     /locus_tag="PKZ53_16340"
     CDS             734..1411
                     /locus_tag="PKZ53_16340"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNJ42058.1"
                     /translation="MSTATVASPLDGHAVTTPRTSAAKLKWIITAVTVVLVIFASVGG
                     FLYFRGKTKASTSKSKQAVKEAESTEAESNSSTSHESDAEEDTSAEDAPPSSKPKPGA
                     APFEAKSLGLSHDGEVEHVIELQPFVVNLADDAEAAYLRMGISVGFGSSEAEAASEEN
                     AKPNPLLTSRLRNAFLALLMTKTSTDLSTQQGKIQLRRELLKVAQAVVHDKNISAVYI
                     TEFLIQK"
     gene            1480..1791
                     /locus_tag="PKZ53_16345"
     CDS             1480..1791
                     /locus_tag="PKZ53_16345"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNJ42059.1"
                     /translation="MGTINTLADLNTSNQATIASWQNFLDLAIPITIELGRTKMTVQE
                     ILGLGLHQVVQLNRSTGEGIDIFASDRCFARGEIIMIGDRTSIRINEILTSQYTQKDP
                     L"
BASE COUNT          557 a          585 c          528 g          487 t
ORIGIN      
        1 ggccgcaacg cgattgccac cgaactggag gggctcaaag cgtcagtcct cacgcttgcc
       61 aaccgtcaaa acccagacgg agcatatttt tttggcggaa cccggcaaac cgcgccacct
      121 tttgacccgg tcacggcgac accggctgga acgccgacat cgccaacgct cattcagatc
      181 gaaccccagg ccaatccaat tcaagccgga gtcactgccg aatcggtgtt tatcaatggc
      241 ccggataccg ttttttcgac ccttgatgag ctggtgacgg cattgcgtgg cacggggaac
      301 cccgtggccg ataaaagcac ccttcaggca ggattgggga aagtaacgac ctttgcccag
      361 caggcaatca gtgcccgaac ggtgatcggc aaaggactca attcggttga aaccgcccag
      421 ggataccttg atcagtattc attttcgttg gatgagaccc taacccaggt ggcgggtgcc
      481 gattttgcag aatcggccct ccaattgaca caggcaaacc aggccctgga ggcgaccctg
      541 caaagcgggg cgagtattgg gcgcaagagt ttgcttgatt tcctggggta acccgtttgg
      601 ggccggtgtg cgatacggca tcaacatcca aaccgaagaa ctatttcttt gaaaaagatt
      661 ggatttgaaa aaaaagactg gtttctcaac cctgagcctg gaatggcatt actcatttga
      721 ttttggaggc gttatgtcta ccgcaactgt tgcttcaccg ctcgacggtc acgctgtcac
      781 cacacctcgt acatcggcgg ccaaattaaa atggatcatt accgccgtga cggtggttct
      841 ggtgattttc gccagcgttg gaggattttt gtattttcgc ggaaaaacca aagccagtac
      901 cagtaaatca aaacaggccg tcaaagaagc tgaatcaacc gaggctgaat caaattcctc
      961 aaccagccac gaatctgatg ctgaggaaga cacttctgcc gaagacgccc ccccttcctc
     1021 aaaacccaaa cccggcgctg caccatttga ggcaaagtca cttggactct ctcacgatgg
     1081 tgaagttgaa catgtgattg aactccaacc gtttgtggtc aatctggctg atgacgctga
     1141 ggccgcttac ctgcggatgg gcatcagtgt tgggtttgga agcagtgagg ctgaagccgc
     1201 atctgaagag aatgcaaagc ccaatccctt attgacctcc cggctacgaa atgcgttttt
     1261 ggcgctgttg atgaccaaaa cctccactga tctttcgacc cagcagggaa agatccaact
     1321 acggcgcgaa ctgctcaaag tcgctcaagc ggttgttcac gacaaaaaca tcagcgcggt
     1381 atacatcacc gaatttttga ttcagaaata aaggcgatgg cctgccattg cctgttcacc
     1441 aggatggttt taccccaaga agaacggaga aatcccacaa tgggcacgat caatacactt
     1501 gctgatctca ataccagtaa ccaggccacg attgcctcct ggcagaattt cctggacctg
     1561 gcaattccga tcaccattga acttggccgg accaaaatga cagttcagga aattttgggt
     1621 ctgggcctgc atcaagtggt ccagttaaac cgctcgaccg gagagggaat tgatattttt
     1681 gcctctgatc ggtgttttgc ccgtggcgaa atcatcatga tcggagatcg aaccagcatc
     1741 cggatcaatg aaatcctcac ttcgcagtac acccaaaaag atccgttata gcctggtatc
     1801 agcctccagt caggcatcag tcattcacga aaatcagcta tagctcttca gaaaaagagt
     1861 tccgcctggg aacgccggca tcctgccggc aactgattgg atctgaaccg cctggtgccg
     1921 gcaggatgcc ggcgttccca ggaaatagtt atctaaaaaa ctatcattga atcacttgat
     1981 tgatttttca tccacaaagt tcattgctgg ctctaaccca accgagacct gttatgccaa
     2041 acacccgtgg tttccaattc aaaatgatgc tgatgattgc ggccctgttt ttgggaatct
     2101 cagccaatct ggcagtaaat gctgccggga ctgccacccc atcacctgac gccccgc
//
LOCUS       DAOQDO010001108         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48543_length_2156_cov_3.601142, whole genome shotgun sequence.
ACCESSION   DAOQDO010001108 DAOQDO010000000
VERSION     DAOQDO010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48543_length_2156_cov_3.601142"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKZ53_16350"
     CDS             <1..1011
                     /locus_tag="PKZ53_16350"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNJ42060.1"
                     /translation="VDFAYGRRGSTLKTGTKTQKPVPYAEAKEVYNRLLAEKTSKGYS
                     PGEEGLAYVQTEHEGRISGVQCQLLNPIEETELEKFVTDPAWCFQEKHDGKRMLIRCL
                     ENGLVEGINRKGLVVGIPSVFATSLANVGTPVILDGEAVGEKLFVFDILQFPGANDDG
                     SEDVRSWNYADRIGLLKQLESKLVGTAVEIVPTAQTKEEKEALVQRLKTSGKEGLVIK
                     RLDAPYAAGRPASGGNQLKYKFTESVSCVVVSHNQKRSVNLGLLDDDGTLVEAGSVTI
                     LPNFQIPPLNSIVEVRYLYAHAQSGKLYQPVYLGERDDIEPIHCRKTQLKFKAPIEDD
                     EG"
     gene            1245..>2156
                     /locus_tag="PKZ53_16355"
     CDS             1245..>2156
                     /locus_tag="PKZ53_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNJ42061.1"
                     /translation="MLISDGLTAELHVLAALITGSANDETRRRLASQLVSRLMVVYFI
                     QKKGLLDGNQEYLRHKLNQCRQIGEQDNFVAFLEMLCFDGLSTPEPNRSPETRLRLGN
                     VPFLYTEMFGKPSSEALYCQPIQVPDAIFERLFEFFDQYEWQMDGDWSDSPWVVTPDV
                     LGWLLEQRINQKQLGAYFTRDDTTRYISQNTIIPLLFQQVQKEFSTVFEPHGQLWQLL
                     SSHPDRYIYPAIKKGVHLSIPRSVAAGIDDPSLRTQWNEPAAADYALPTETWREVIAR
                     RQRYGALRTKLETGAIHDINQLITENLN"
BASE COUNT          562 a          530 c          555 g          509 t
ORIGIN      
        1 gttgattttg cttatggtcg ccgtggttcg acgctcaaaa ccgggaccaa aacccaaaag
       61 ccggtgccct acgccgaggc aaaggaggtc tacaaccggt tgctggcaga gaaaaccagc
      121 aaaggctata gccctggtga agaagggctg gcctacgttc aaaccgaaca cgaaggccga
      181 atttcaggcg tccagtgcca gttgctcaat ccaatcgaag aaaccgaact tgaaaaattt
      241 gtgactgacc cggcgtggtg ttttcaggaa aagcacgatg gcaaacggat gctgattcga
      301 tgtttggaaa atgggctcgt cgaaggcatc aaccggaaag ggctggtggt cggaatcccg
      361 agtgtatttg caacaagcct ggccaatgtc ggaacgccgg tcatcctgga tggagaagcc
      421 gtcggcgaga agttgtttgt gttcgatatt ttgcaatttc ccggagccaa tgacgatggt
      481 tccgaggacg tgcgttcgtg gaactacgct gaccgaattg ggcttctgaa acagcttgaa
      541 tctaagctgg tcggcacggc ggttgaaatc gttccgacgg cacagacgaa agaggaaaaa
      601 gaagcgctag ttcaacgcct caaaaccagt ggaaaagaag gactggtcat taaacgattg
      661 gatgcacctt acgccgctgg ccgtccggcg tcgggtggga atcagctcaa atacaaattc
      721 actgaaagcg tgtcgtgcgt cgtggtcagc cataaccaaa aacggagtgt caacctggga
      781 ctgcttgacg atgacggaac tctggttgaa gccgggagcg tgacgattct tcccaatttc
      841 cagattccac cgctgaactc aattgtcgaa gtccgctatc tgtatgccca cgcccagtca
      901 ggcaagctgt accagcccgt ctatcttggt gagcgtgacg acattgagcc gattcattgc
      961 cggaagacac aactgaaatt caaagcccca atcgaagatg atgaagggta agatgtacaa
     1021 gcatttttga accacagagg acacagagaa gcacagagga aaacaaaata agtaccgtat
     1081 catgagtttc ctgagatatt ggatttggta agtacttgat tttttccgtg ttttccgtgt
     1141 tttccgtggt taaactatct gcaaattctc ggcaaaatac ttacctctgt gtcctctgtg
     1201 gtgaaatttg aatagctact tctactctta aagcacacct ctccatgctg atttctgatg
     1261 gcttgaccgc tgaacttcac gtgctggcgg ctttgatcac aggcagtgca aatgatgaaa
     1321 cacgccgccg attggcttca caactggttt cgcggttgat ggttgtgtat ttcatccaga
     1381 agaagggatt gcttgacggc aatcaggagt accttcgcca taaactcaac cagtgccgtc
     1441 aaataggcga gcaagacaac tttgtggcgt ttctcgaaat gctatgtttt gacggtcttt
     1501 caacgcccga acccaaccgg tcgcccgaaa cccggttgag gttgggcaac gtgccgtttc
     1561 tctacacgga gatgtttggc aagccgtcat ccgaggcgct gtactgtcaa ccgattcagg
     1621 ttccggatgc gatttttgaa cggctttttg agttctttga tcagtatgaa tggcagatgg
     1681 atggagactg gtcagatagc ccctgggttg tgactcccga tgtgctgggc tggttgttag
     1741 aacagcgcat caatcaaaag cagttggggg cctactttac ccgagacgac acaacccgct
     1801 acatcagcca aaacacgatc attccattgc tgttccaaca ggtccaaaaa gagttctcaa
     1861 ccgtctttga acctcacggc cagctctggc aactgctttc atcacatcca gatcgctata
     1921 tctatccggc aatcaaaaaa ggagttcatc tctcaatccc caggtcagtt gcggcaggta
     1981 ttgatgatcc ttcgttgcgc acgcagtgga atgagccagc cgctgccgat tatgcgttgc
     2041 cgaccgaaac ctggcgcgaa gtcattgccc gccgtcagcg atatggtgcg ctccgaacaa
     2101 aactggaaac tggcgccatc cacgacatca atcaactcat tactgaaaat ctaaat
//
LOCUS       DAOQDO010001109         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48545_length_2156_cov_3.421228, whole genome shotgun sequence.
ACCESSION   DAOQDO010001109 DAOQDO010000000
VERSION     DAOQDO010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48545_length_2156_cov_3.421228"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKZ53_16360"
     CDS             <1..703
                     /locus_tag="PKZ53_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ42062.1"
                     /translation="TVAAAANLAQTGAIVTLGITPTRPETGYGYIETGNPALVQAGKQ
                     YFKVSRFVEKPDVETATRYVTSGRFVWNSGIFVFSAKTILAEIEAHLPSLYAGLLEIE
                     ATIGNPDLYAATLNRVYGELDSVSIDYGVMEKTTAPLFVFQSDFGWSDVGSWQALYEL
                     RTPEHDDQGNLLIGTGIALHSSGNLVYSKTERQVALLGVEDLVIVDLPDTLLVSRRSL
                     SQDVKLVPQALKTSG"
     gene            complement(1180..1953)
                     /locus_tag="PKZ53_16365"
     CDS             complement(1180..1953)
                     /locus_tag="PKZ53_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ42063.1"
                     /translation="MLKIYVVDDEPLAVRRLVRMLESTSRVEVVGSTTDPETAIEELS
                     TLTLDAVFLDIQMPGYNGFEVLSHLPHQPLVVFTTAFDQYALQAFEVNSIDYLLKPIE
                     AAQLDRALAKLERLRAHQSPHPSDHLQSVISQLAQTLAVPTRQFPERIASRVGERIQF
                     LDLATVTHFFSQDKLTFAVANGKEWVVDYTISKLEEKLDPARFLRIHRSTLVNLSFVE
                     ELHTWFAGQLMVRLKDASKTELQVARDRVRELKERMGMF"
     gene            complement(1947..>2156)
                     /locus_tag="PKZ53_16370"
     CDS             complement(1947..>2156)
                     /locus_tag="PKZ53_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42064.1"
                     /translation="STDAGVTIEIKDDGPGFDSKDIQPGHGIDNVKSRLQVLFGDEAD
                     FQISSHSTGTSVSVSVPFHRRTEVC"
BASE COUNT          516 a          559 c          575 g          506 t
ORIGIN      
        1 taccgttgcc gccgccgcaa acctggctca aaccggcgcg attgtgacac tgggcattac
       61 cccaacccga ccagaaaccg gatacggata tattgaaacc ggcaacccgg cactggttca
      121 ggctggaaaa caatatttca aagtcagccg gtttgttgaa aaacccgatg ttgaaaccgc
      181 aacccggtat gtcaccagtg gccggtttgt atggaatagc ggaatttttg tcttctcagc
      241 caaaacaatc ctggctgaga tcgaagcgca cctcccatct ttatatgctg gattgctcga
      301 aatcgaagcc acgattggaa atcctgacct gtacgcagca acgctgaatc gggtctatgg
      361 tgagcttgat tcggtttcga ttgattatgg cgtgatggaa aaaaccaccg ccccgctctt
      421 tgtgtttcag tctgattttg ggtggagcga tgttggaagc tggcaggcgc tgtatgaact
      481 ccgaaccccg gaacacgacg atcaggggaa ccttcttatc ggcacgggaa tcgccctgca
      541 ctcatcagga aatctggtgt attccaaaac cgaacggcaa gtggccctgc ttggcgttga
      601 agacctcgtc attgtggacc tcccggatac gctactggtc tcacgccgca gtttgtccca
      661 ggatgtgaaa ctggtgccgc aggcattgaa gacttcgggc tgaagaagtt gggctgaaga
      721 agttgggctg aagattcgca agctcagggc ggaagacatg gggccaaggg agcgttaaaa
      781 tttaacttcc cgttttcgtt ggagaaggga tttggattga gcatcctgaa aggctgcagt
      841 tcggatagcc ggtgattgcg aggttttggg agctaccacc gggaaaagcg ttcgggttcc
      901 tcatcctccg agctgcttgc ccgcgcccta cagggcgcgg gcaagcagct cggaggaaca
      961 acgggtggcc ttcgtgaccg gtggtaggcc caaagcggcc aaccgaccgg ctatctgaac
     1021 ggcaacgctt cgcggtgcaa accccaaaaa cctacatttg gctaaagaaa ataggatttc
     1081 agccctcaac ccgaagtctt cagccctgag cttgcgaatc ttcagccctg gtttttcagc
     1141 ccgctttctt cagccctgtt tttttcagcc ctggttttct caaaacattc ccattcgttc
     1201 cttcaattcg cggactcgat cacgcgccac ctggagttcg gttttggagg cgtcttttaa
     1261 tctgaccatg agttgacccg caaaccaggt gtggagttct tcgacaaatg aaagattgac
     1321 cagcgttgac cgatgaatcc gcagaaaccg tgccgggtcg agtttttctt caagttttga
     1381 aatcgtgtaa tcaaccaccc actccttgcc attggccacg gcaaaggtga gtttatcctg
     1441 cgagaaaaaa tgcgtcaccg tggccaggtc aagaaactgg attcgttcgc cgacgcgtga
     1501 cgcaatgcgt tctgggaact ggcgtgtcgg gacagccagt gtttgggcca gttgcgaaat
     1561 caccgattgc agatggtcag atggatgtgg tgactggtgg gcacgcagtc gctcaagttt
     1621 cgccagggcc cggtcaagct gggcggcttc gattggcttg agtaaataat caatcgaatt
     1681 gacttcaaac gcctgtaacg catactggtc aaatgccgtg gtgaagacaa ccagcggctg
     1741 atgaggcagg tgcgatagta cttcaaaccc gttgtatccg ggcatttgaa tatccagaaa
     1801 aactgcatca agggtcaggg tcgagagttc ttcaatcgcg gtttccgggt cagtcgtgct
     1861 tccaacaact tcgacccggc tggttgattc caacatccgc accaggcgcc gaacagccag
     1921 tggttcatcg tcaacaacat atattttcag cacacttcag ttctccgatg aaacggaacc
     1981 gagaccgaga cactggttcc agttgaatgg gacgaaattt gaaaatcagc ctcatcgcca
     2041 aacaaaacct gcaacctcga ctttacattg tcaatgccgt ggccgggctg aatgtctttg
     2101 gaatcaaacc ctgggccgtc atctttgatc tcaatggtca cacccgcgtc agttga
//
LOCUS       DAOQDO010001110         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48645_length_2154_cov_2.686041, whole genome shotgun sequence.
ACCESSION   DAOQDO010001110 DAOQDO010000000
VERSION     DAOQDO010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48645_length_2154_cov_2.686041"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..>2154)
                     /locus_tag="PKZ53_16375"
     CDS             complement(159..>2154)
                     /locus_tag="PKZ53_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ42065.1"
                     /translation="VEGLAMRIVSGDVPESLKNKRLITLDLGAMLAGAKYRGEFEDRL
                     KAVLKEVIRAEGQIVLFIDELHTLVGAGAGEGALDASNMLKPALARGELRCVGATTLN
                     EYKKHVEKDAALERRFQPVFVAEPDVQDTISILRGLKERYEIHHGVRIKDAAIVAAAH
                     LSNRYIADRFLPDKAIDLIDEAASRLRIEIDSLPTEIDEVEREITQREIERQALQREE
                     DVDARERLKRIELEISEMKEKSSGLKARWQSEKEVIQRLRENKEKLEELKIEADRFER
                     AGDYAKVAEIRYGKMAELQRQVEANRARLVELQKTGRMLKEEVDEEDVALVIAKWTGI
                     PVSKMLESEMQKLLHMEERMQQRVIGQNEALEAVANAVRRSRAGLQDPNRPIGSFIFL
                     GPTGVGKTETARGLAEFLFDDEHAMVRLDMSEYMEKHSVARLIGAPPGYIGYEEGGQL
                     TEAVRRRPYSVILFDEIEKAHPDVFNVLLQILDDGRLTDGKGRAVDFKNVVIIMTSNI
                     GSREILEFSGRKETETQMREAVLSHLRNAFKPEFLNRIDDIVIFHSLGRQELYRIVEV
                     QLEKLRKMLAERNVTLVLTPEATEYLASDGFNPTYGARPLKRSIQNLLQNQLAVRLLR
                     GEIGPGQTVKVDYDSREEKLTFTAVTATGSDAQAAASKGF"
BASE COUNT          470 a          619 c          505 g          560 t
ORIGIN      
        1 ttgaatgttc atcaattcac gaaatggttc aaatcgttgg attgacatac ttttcctcca
       61 cactttgttt ttttgactga ggaatccagg gctgaggtga acggccaatg cgttcgaaaa
      121 accctcaggc cctcagccca ccgcctcaac tctgggaact aaaagccttt tgaagccgcc
      181 gcctgggcat cagatccggt ggcggtgaca gccgtaaagg ttaacttctc ttcccggctg
      241 tcataatcaa ccttgacggt ttgcccaggt ccaatctcgc cgcgtaacaa ccgcacggcc
      301 agttgatttt gcaacagatt ttgaattgac cgtttgagcg gacgtgcacc ataggtcggg
      361 ttaaacccat cggaagcaag gtattccgtc gcttcagggg ttaacaccag cgtgacgttg
      421 cgttcggcca gcatctttcg caatttctca agctgcactt cgacaatccg atacaattcc
      481 tgacgtccga gtgaatggaa aatcacgata tcatcaattc ggttcagaaa ttctggctta
      541 aaggcattgc gcaagtggga aaggaccgct tcgcgcatct gcgtttcagt ttcttttctt
      601 ccgctaaatt caaggatttc acggctgccg atatttgaag tcataatgat gacgacgttt
      661 ttgaaatcca ccgcgcggcc ttttccgtcg gtcagccggc catcatcgag gatttgcagc
      721 aacacgttga acacatccgg atgggccttc tcaatttcgt caaagagaat gaccgaataa
      781 ggccgtcgcc gaacggcttc ggtcaattga ccaccttcct cgtagccaat atatcccgga
      841 ggcgcaccaa tcagacgggc gaccgaatgc ttctccatat attccgacat atcgaggcgc
      901 accatcgcgt gttcgtcatc aaacagaaat tcggccagtc cccgggcggt ctcggtcttc
      961 ccaaccccgg ttggcccaag gaagataaat gacccgattg gccggtttgg atcctgcagg
     1021 ccggcacgcg agcgacgaac cgcattggcc acggcttcca gggcttcatt ttgaccaatg
     1081 acccgttgct gcatccgctc ttccatatgc agcagttttt gcatttcgct ttccagcatt
     1141 tttgaaactg gaatcccggt ccatttggca atgaccagtg cgacatcttc ttcatcaact
     1201 tcttctttga gcatacgccc ggtcttttgc aactcaacca gccgtgcccg gttggcttca
     1261 acctgccgct ggagttcagc catcttgccg taccggattt cagccacttt ggcgtaatcg
     1321 ccagcccgtt caaagcggtc ggcttcgatt ttgagttcct ccagtttttc tttgttttcg
     1381 cgcagacgct gaatgacttc tttttcagat tgccaccggg ctttcaaccc ggagcttttt
     1441 tccttcatct ctgaaatttc cagctcaatt cgctttaacc gctcacgggc atccacatct
     1501 tcttcacgct gaagagcctg gcgttcaatc tcacgttgtg tgatttcgcg ttcaacttca
     1561 tcaatttcag ttggcaggct gtcaatctca atccgtaacc gcgatgcagc ctcgtcaatc
     1621 agatcaatcg ctttgtcagg taagaaccgg tcggcaatat agcgatttga gaggtgcgca
     1681 gcggcaacaa tcgccgcgtc cttaatgcgc acgccgtgat ggatttcata gcgttctttc
     1741 aggccgcgca aaatggaaat cgtgtcctgc acatcgggtt cggccacaaa cactggctgg
     1801 aatcgccgtt ccagcgccgc atctttttca acgtgctttt tgtattcatt cagggttgtg
     1861 gcgccgacgc accgcaattc gccacgggcc agagctggct tgagcatatt ggaagcatcc
     1921 agcgcgcctt ccccggcgcc tgcaccaacc agcgtatgta actcgtcaat aaagaggaca
     1981 atctgccctt cggcccggat gacttctttc aacaccgctt tgagacggtc ttcaaattcg
     2041 ccccggtatt tggcgcccgc cagcatggcg ccgagatcca gcgtaatcag gcgcttgttt
     2101 ttgagggatt ccggaacatc acctgagaca atccgcatgg ccagtccttc aaca
//
LOCUS       DAOQDO010001111         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48667_length_2153_cov_3.931840, whole genome shotgun sequence.
ACCESSION   DAOQDO010001111 DAOQDO010000000
VERSION     DAOQDO010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48667_length_2153_cov_3.931840"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKZ53_16380"
     CDS             complement(<1..259)
                     /locus_tag="PKZ53_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42066.1"
                     /translation="MLLQDSLLLQDSLLLQDSLLLQEAFSTLQPPLPLQEFLPAQPLS
                     PLLQPPCPLHSFLPLHSWALLQEAFSTLQPPLPLQEFLPAQP"
     gene            367..1230
                     /locus_tag="PKZ53_16385"
     CDS             367..1230
                     /locus_tag="PKZ53_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNJ42067.1"
                     /translation="MTTPNRWNFPDLGFGVGLRTVHFTHILENQPPVDWFEIISENFM
                     ETGGRPMFILEQVAERYPIIMHGVSMSIGSTDPLNMDYLKKLKALADRIGSTWVSDHL
                     CWTGVHGRNTHDLLPMPYTEEALKHTIERARIVSDLLERPLILENPSSYVEFADSSMP
                     EWEFLARLVEDANCGLLLDVNNVYVSSFNHNFDPTLYIDAIPADRVVQYHLAGHTNKG
                     THILDTHSDHVIDEVWALYRQATAKTGGRATLLEWDDDIPEFDVVHAEVLKARQYLPE
                     PVLAVGGGYGD"
     gene            1220..2071
                     /locus_tag="PKZ53_16390"
     CDS             1220..2071
                     /locus_tag="PKZ53_16390"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNJ42068.1"
                     /translation="MVTEVPLSQLQTWMQSYIVFPGATSEAMEAASESAGLEEEKATS
                     IILPSKTLTGQERLNIYRGMYLLRMNDALSADFFAVEHFLGEDDFFDLVARYVQQFPS
                     RSFTLNRLSDNFPEYIQTDTQVPNRAFLYDLARLELAVTQVFDAELSSILTTEMIQDI
                     PADAWEFARFRPVHGFQVLAFRYPVNAYLEAVRANTHRPKIKRQDTWVAVCRRGYSVW
                     RFDLTRPEYEMLMALVSGMPLGEAILATTRRFSSRGVKEQIFQWFRGWVTQGFFQSVE
                     LEKAQSE"
BASE COUNT          522 a          536 c          553 g          542 t
ORIGIN      
        1 acggctgcgc tggcaaaaac tcctgcaaag gcaaaggcgg ctgcaaagta gaaaaggctt
       61 cctgcaagag cgcccatgaa tgcaaaggca agaatgagtg caaagggcag ggcggctgca
      121 agagcggcga caacggctgc gctggcaaaa actcctgcaa aggcaaaggc ggctgcaaag
      181 tagaaaaagc ttcctgcaag agcaaggaat cctgcaagag caaagaatct tgcaagagca
      241 aagaatcttg caaaagcaaa agctcctgca aaagccgata attagtaagt tttcttctct
      301 tccgtggccg agggtgccct ttctcaggct gtaccttcgg ccacatttcg tcttagtgga
      361 agttttatga ccacacctaa tcgttggaat ttcccggatc ttgggttcgg ggttggcctg
      421 cgtactgttc attttaccca cattctcgaa aatcaacccc cagttgattg gtttgaaatc
      481 atctctgaaa attttatgga aaccggcggt cgccccatgt tcatcctgga acaggtggct
      541 gagcgctatc caattattat gcacggcgtg tcgatgtcga ttggcagcac cgatccgctc
      601 aatatggact atctcaaaaa actcaaggca ctggcggacc gcattggcag cacctgggtt
      661 tctgaccatc tgtgctggac tggcgtccat gggcgaaata cccatgattt gctgccgatg
      721 ccctatacgg aagaagccct caagcacaca attgagcgcg ctcgcattgt ctcggatttg
      781 ctagagcgtc cgctgattct ggaaaaccct tcgtcctatg ttgaatttgc cgattcatca
      841 atgccggaat gggaattttt ggcgcgtctg gttgaagacg ccaattgcgg cttgctgctg
      901 gatgtcaaca atgtctacgt cagctcgttt aaccataatt ttgacccaac cctgtatatt
      961 gatgcgattc cggctgatcg ggtcgtccag tatcacctgg ccggacatac caacaaaggc
     1021 acgcatatcc tcgacacgca cagcgaccat gtgattgacg aagtgtgggc tctctatcgt
     1081 caagccaccg ctaaaaccgg cggacgagcc accttgcttg agtgggatga tgacattcca
     1141 gagtttgatg tggttcatgc cgaagtctta aaggcccgtc agtatcttcc ggaaccagtt
     1201 ctggccgtgg gaggtggata tggtgactga agttccatta agccagctcc agacctggat
     1261 gcaaagctac attgtgtttc caggtgcgac cagtgaagca atggaagctg ccagcgagag
     1321 tgctggtctt gaagaagaaa aagccacttc aattattctg ccgtcgaaaa ccctgaccgg
     1381 acaggaacgc ctcaacattt atcgcggcat gtatctcttg cggatgaatg atgccctgtc
     1441 ggctgatttt tttgccgttg aacattttct gggcgaagat gatttttttg acctggttgc
     1501 ccgctatgtc cagcaatttc cctcccgcag tttcacacta aaccggctga gtgacaattt
     1561 tcccgaatat attcagactg atacccaggt gccgaatcgc gccttcctct atgatctggc
     1621 acggttggaa ctggcagtaa ctcaggtctt tgatgctgaa ctttcatcaa ttctgaccac
     1681 agaaatgatc caggatatcc cggctgatgc ctgggaattt gcccggtttc gtcccgttca
     1741 tggatttcaa gttctggcgt tccgctatcc ggtcaatgcg tatctggaag ccgtgcgagc
     1801 taatacccac cgtccaaaaa tcaaacgtca ggatacctgg gtggctgtct gccgccgtgg
     1861 gtacagtgtc tggcgttttg atttaactcg acctgaatat gaaatgttga tggctctggt
     1921 ctctggaatg ccgcttggtg aagcaattct tgccaccacg cgccgatttt caagccgggg
     1981 tgtaaaagag cagatttttc agtggtttcg aggctgggta acccagggat ttttccaatc
     2041 tgtcgaattg gagaaagccc agagtgagtg atttaaaaca aatcgcggtt ctgattttgg
     2101 ctgccggcgc gtcgcgacgt ttgggacacc ccaaacaatt gctaccgatt ggc
//
LOCUS       DAOQDO010001112         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48677_length_2153_cov_2.983794, whole genome shotgun sequence.
ACCESSION   DAOQDO010001112 DAOQDO010000000
VERSION     DAOQDO010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48677_length_2153_cov_2.983794"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1075)
                     /locus_tag="PKZ53_16395"
     CDS             complement(62..1075)
                     /locus_tag="PKZ53_16395"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNJ42069.1"
                     /translation="MPETVLDLSGIETVYPPTTRVADSFAQALFGHSSQQSITTLKAL
                     LSQYTGVDAGRILVTSGEDDLLTLLLTNLMGKGDYIAVPEPSLGTHRFQAEATGVGVL
                     DTGRDKQFRVLTDSLLSVATAPEVKMIFLASPNNPTGTLTSPKTIQRILDVQKLTVID
                     ETYFEFSHQSCIGLLASHPNLVILRSFKWAAMAAYKIAYAVAAPEIVARLEATLPGTL
                     VSPMAISAACSSLEDQALLLRNVKRLIEERDRLYETLHHISGVHPFASQANFLYCLFP
                     GRDGRQVANALFRQGVKVKHYTGSVYQSGIRVRIGFPDDNERFLSALRTVLTEIPTGN
                     EPG"
     gene            1236..1460
                     /locus_tag="PKZ53_16400"
     CDS             1236..1460
                     /locus_tag="PKZ53_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42070.1"
                     /translation="MTSSQQSLAGVTDGEGYSVTCHRAPFPGKSRPKVHVSTGLNMSR
                     PPRAATVCQTAVIWKDQFFLYTQIILWKQL"
     gene            complement(1480..>2153)
                     /locus_tag="PKZ53_16405"
     CDS             complement(1480..>2153)
                     /locus_tag="PKZ53_16405"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNJ42071.1"
                     /translation="LLELRENLFDQRSRLRPVRDLADLEYGPDGQNLAADNQLGVWIG
                     LDTLDWLMEPSPIETINGLMSNGLQMVIVNEKSARFLLDSGPALVEAIQSPGLWLLVG
                     ELDGKRRERLLPLLKRPALITDADLSDDELKALAEAGHLVVMMPRSTDQKNDRRKKPL
                     TPKAVVERLVHILQVVGEDKVILSTPDVSQRLVLIEELVRQNLTPGQVEKVFGLNFKR
                     FYAVP"
BASE COUNT          527 a          575 c          536 g          515 t
ORIGIN      
        1 tctcaattct tcattctcaa ttcttcattc ttcattcttc attcttcatt cttcaatgtc
       61 ttcagcccgg ctcattcccg gttggaattt cagttaaaac agtgcgaagc gccgaaagaa
      121 accgttcgtt gtcgtcagga aagccaatcc gaacccgaat tccactctga tataccgacc
      181 cagtgtaatg tttgaccttt accccctggc gaaagagcgc gtttgcgacc tgccggccat
      241 cacgacctgg gaagaggcaa tacaggaaat tggcctgtga ggcaaaagga tgaactcccg
      301 aaatgtggtg cagggtttca tagagtcggt cacgttcctc aatcaggcgt ttcacattcc
      361 gcaacaaaag tgcctgatct tcgagtgagg aacaggccgc agaaatggcc atcggggaaa
      421 ccagggtacc aggcagggtc gcttccaatc gggcgacgat ttcaggcgcc gccaccgcat
      481 aggcaatttt ataagcggcc atcgcagccc atttgaagct ccgcaaaatc accaggttgg
      541 gatgtgatgc cagcaacccg atacaactct ggtgtgaaaa ctcaaagtag gtctcatcaa
      601 tcaccgtcag tttttggacg tcgaggatgc gctggatggt tttgggggag gtcaatgtgc
      661 cagttggatt gttagggctg gccagaaaaa tcattttgac ttccggggcc gtggcaaccg
      721 acagcaggga gtcagtcagg acccgaaact gtttatcgcg tccggtatca agcacaccaa
      781 cgccggtggc ttctgcctgg aagcgatggg taccaagcga cggttctgga accgcgatgt
      841 agtccccttt tcccatcaaa ttggtcaata acagtgtaag caggtcatct tcgcccgaag
      901 tgaccagaat ccgcccggca tcaaccccgg tatactgact gagcagcgct tttaaagtgg
      961 tgattgactg ctgcgaggaa tggccaaaca gcgcctgagc aaacgaatca gccacccgag
     1021 tcgtcggcgg gtagacagtt tcgattccac tcaggtcaag aactgtttct ggcattgtgg
     1081 cgtgctcgct tgtgtgtgac aacgtgtgct tcagccagaa agaactgttc acagttttga
     1141 ttcacccgaa aaaagcggca cttcggatgt atccgaggtt gaagtgacag ttttattgtc
     1201 tgaaagcagt tcagatagga aggtatgcgg acttgatgac atcctcccag caatcacttg
     1261 ctggtgtcac agatggtgag gggtactcag tcacctgtca ccgggcacca tttccgggga
     1321 aaagccgccc aaaggttcac gtttcaacgg gcctcaatat gagtcgccct ccacgtgccg
     1381 caacggtttg tcaaaccgcc gtgatctgga aagatcaatt ttttctttat acacagatta
     1441 tcttatggaa gcaactctaa ctctatcaga accagttcgt cacggtacgg cataaaaccg
     1501 tttgaaattt aaaccgaaca ctttctcaac ctgacccggc gtcagatttt gtctgaccag
     1561 ttcttcaatg agcaccagac gctgtgacac gtcaggcgta ctcagaatca ccttatcttc
     1621 accaaccact tgcagaatat ggaccaggcg ttcgacgacg gcttttgggg tgaggggctt
     1681 tttccgcctg tcatttttct gatcggtact gcggggcatc ataaccacca gatgaccagc
     1741 ttcggccaac gctttgagtt cgtcatctga taaatcagca tcggtaatca gggctgggcg
     1801 tttcagcaag ggcaagagtc gttcccgacg ctttccatcc agttcgccaa ccagaagcca
     1861 cagcccaggc gattggatcg cttccaccag ggctggcccg ctatcgagca ggaatctggc
     1921 acttttttca ttcacaatca ccatctgcaa gccattgctc atcaatccat tgatggtctc
     1981 aatcggtgat ggttccatca gccagtccag ggtatcaagc ccaatccaca caccaagctg
     2041 gttatcagcc gccagattct gaccgtcagg accatattcc agatcggcca gatcgcgaac
     2101 tggacgcaga cggctccgct ggtcaaacag gttttcgcgc agttcgagca gtt
//
LOCUS       DAOQDO010001113         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48890_length_2148_cov_3.394649, whole genome shotgun sequence.
ACCESSION   DAOQDO010001113 DAOQDO010000000
VERSION     DAOQDO010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48890_length_2148_cov_3.394649"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..354
                     /gene="trxA"
                     /locus_tag="PKZ53_16410"
     CDS             7..354
                     /gene="trxA"
                     /locus_tag="PKZ53_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNJ42072.1"
                     /translation="MTIENFEPEVLESPVPVLVDFYASWCPPCQMLSPVLEKLAVQYQ
                     GKAKIVKVNTEEQPDLAAVFRIRSIPTLYFFAGGKAVDTAVGFVQPNLLQHKLNTLIS
                     NMNSTQNELRRAI"
     gene            351..566
                     /locus_tag="PKZ53_16415"
     CDS             351..566
                     /locus_tag="PKZ53_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNJ42073.1"
                     /translation="MNIERALRLIAGFFVLLSVALGVWHSPYWFLFTAFVGLNLLHSG
                     ITNWCPMVWILEKAGLPHCGTPVEIKH"
     gene            686..2089
                     /locus_tag="PKZ53_16420"
     CDS             686..2089
                     /locus_tag="PKZ53_16420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ42074.1"
                     /translation="MRGTHIQHFKTGFWRLRTSAVMVVLVLFQVTIPVWGQTPSEPEG
                     LTLPLAVEIALRSNPGLQAVTTGQTLAQVGTQEARAGRLPTISVSEVVTNGNNPVYVF
                     GSLLEQSRFAEKNFALDSLNNPDPLTNFRTQVQVRYPLFDQRRAQTQIAKAEIGEQQA
                     SAQREMVEQRLRFEVIRAYFGLLVAQANQRVADQAVETAGAEAKRVKDMFDSGMVVAS
                     DLLSIETQLAEFRQQQIQAKGDIQTAQAALNTVLGVAIDRQQSVTGTLEGQSFTVLPE
                     AELITSALANHPEYRQAALAVEQRQKDQHGARGEYQPRTDLFATVGNSGHKLVNGSGD
                     YAVGVSVTFDLFKPGRAERVKQADLAREISDYQLQQKANELRFEVVRANQHFLSARAR
                     LEVATKAVSQAQEVLRIVRDRYGAGITTVTEVLRAQTALVRAQTNELFARYDYVVGFA
                     QIQLASGQLKEVGPFVR"
BASE COUNT          493 a          593 c          582 g          480 t
ORIGIN      
        1 caacacatca caatcgaaaa ttttgaaccc gaagtgctgg aatctccggt cccggtgctg
       61 gtggatttct atgcttcctg gtgtccgccc tgtcagatgc tgtcaccagt ccttgaaaaa
      121 ttagccgtcc agtatcaagg caaggccaaa atcgtgaagg tcaacaccga agagcaacct
      181 gatctggccg ccgtttttcg gattcggagt attccaaccc tgtatttctt cgctggtggc
      241 aaagcggttg atacggcagt gggctttgtt caaccaaacc tgctccagca caagctcaac
      301 accctgattt caaacatgaa ctccacccaa aatgagttgc ggagggccat atgaacatcg
      361 aacgcgcctt acgcctgatt gccggatttt ttgtgctgct cagtgtcgca cttggcgtct
      421 ggcacagccc ctactggttt ctctttacag catttgttgg cttaaacctt ttgcattcag
      481 ggattaccaa ctggtgcccg atggtctgga ttctggaaaa agcgggcctt ccgcactgtg
      541 gaaccccggt tgagataaaa cattaaggaa gaagggccca gttaaatact gtatgccggg
      601 gttaaagact gactcatttt cttcagcccg ttttcttcag ccccaagccc gttttcttca
      661 gccccaacac aaaggcaggt ccgctatgcg tggcacgcac atacaacact tcaaaacagg
      721 tttttggcgg ctgcgaacga gtgccgtgat ggttgtcctg gtactttttc aagttaccat
      781 tccggtttgg ggccagacgc catctgaacc cgagggattg accctgccgc tggcggtgga
      841 aattgcgctc cggtccaatc ctggtttgca agcggtgacc acgggtcaga cactggctca
      901 ggttggaacc caggaagcca gagccgggcg actgccaacc atttcagtca gcgaagtcgt
      961 caccaacggc aataacccgg tctatgtttt tggctcgctg ctggaacagt cgcgatttgc
     1021 ggaaaaaaac tttgcgcttg attcattaaa taaccccgat ccactgacca attttcgaac
     1081 gcaggttcag gtgcgatatc cgctctttga ccagcgccga gcccaaaccc agattgccaa
     1141 agctgaaatc ggcgaacaac aggccagcgc ccaacgcgaa atggtcgaac aacgcctccg
     1201 atttgaagtg attcgcgctt atttcggact gctggtggcc caggccaacc agcgcgtggc
     1261 cgaccaggcg gttgagaccg ctggagctga ggcgaaacgg gtcaaggata tgtttgattc
     1321 cgggatggtg gtggcgtctg acctgctttc gattgaaacc caactggctg aatttcgaca
     1381 acagcagata caggcaaaag gcgacatcca gacggctcag gcggcactca acacggttct
     1441 cggcgtagcc attgatcgtc aacagtcagt caccggcaca ctggaaggtc agtcatttac
     1501 cgtcctgcct gaagctgaac tcatcacctc ggcactggcc aaccatccgg aatatcgtca
     1561 ggcggcgctg gcggtcgaac aacgccagaa agaccagcac ggggcgcgtg gtgaatacca
     1621 gccgcgaacc gacctgttcg cgaccgttgg aaatagtgga cacaagctcg tgaatggcag
     1681 cggagattat gccgtcggcg tgagtgtcac gtttgatttg ttcaagcccg gtcgtgccga
     1741 acgcgtcaaa caggccgatc tggcgcgaga gatttcagat taccagctcc aacaaaaagc
     1801 caatgaactg cgctttgaag tcgtgcgcgc caatcagcac ttcctgagtg ccagggctcg
     1861 ccttgaggtg gcgacaaaag cggtgtccca ggctcaggaa gttttacgaa ttgtccgtga
     1921 ccgctatggc gcgggtatca caaccgtcac cgaagttttg cgcgcccaga cggcactggt
     1981 tcgggcgcaa accaatgaac tctttgcccg ctatgactat gtggtgggat ttgcgcagat
     2041 tcaactggcc tccgggcaac tcaaggaggt cgggccgttt gtacggtaac accaaatccg
     2101 ggttccgggt tccgggttcc gggttccggg ttccgggttc cgggttcc
//
LOCUS       DAOQDO010001114         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_48935_length_2147_cov_3.178298, whole genome shotgun sequence.
ACCESSION   DAOQDO010001114 DAOQDO010000000
VERSION     DAOQDO010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48935_length_2147_cov_3.178298"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..290)
                     /locus_tag="PKZ53_16425"
     CDS             complement(54..290)
                     /locus_tag="PKZ53_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42075.1"
                     /translation="MTVPNDEVVFGFWFLVLGSWFLVLGSWFLVFGFWFLVLGSWFLV
                     LGSWKAIESSAQCLQPSAPSPFSSARSLQPQFPA"
     gene            289..2055
                     /locus_tag="PKZ53_16430"
     CDS             289..2055
                     /locus_tag="PKZ53_16430"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HNJ42076.1"
                     /translation="MGFRFRKSIKIAPGLKVNLSTKGMSVTVGGQGASVNVGSAGTFL
                     NLGVPGTGLSYRTKVSSNRRKHPATPPPPTPLPPPPGTQPGWSQQPSPPGVQPTLVPA
                     SIGLNADSRICFYNQYGQVVPDTWLDQLVSQNWKAVTEVLEREAAAYNDDSGFDLHLE
                     TPSPRKSFDLPELILAGSVPAPPEIFDFSEPIPLPPELKTPGFLGGLMKSKRDQIEAE
                     NNRLREAYVQAQREWERRRTAHTTYKTEVMADYQEQLASYKRLKADITERNRVRQSAL
                     TNGREGNPEAMEDLLAEVFKEIDWFRETTASFEVTSDAQTVWVDVDFPEIEDMPAYTA
                     TVDFPGFRLNVQPRSTYRRQKEYIHHIHSVAFRVLGEIFWALPKVTSVVLSGYSQRPN
                     AQTGVMEDQYLLSVQVPRVEWIKVDFLNLKGVDVFSFFEQFKLRRKCSRTAIAAPITP
                     FRTIADLHDWMPTDCMLSRKLELAALTREEKESMSWLDRRQLSSLTPEELEIRQVTAE
                     KLFGRFVAERINISRIEKGDSEEIVKAIIGSPTTTEAKFLQGKNHVTWKYVGSKPHQI
                     KFTVNFENGAVVSWEGNLPLGG"
BASE COUNT          587 a          559 c          546 g          455 t
ORIGIN      
        1 gaatcaacac tgctcccagt atccggcagg tggaatcagt ctgtcgaatt aggctatgct
       61 gggaattgag gctgaagact tcgggctgaa gaaaacgggc ttggggctga gggctgaaga
      121 cattgggctg aagattctat tgccttccaa gaaccaagca ccaagaacca agaaccaagc
      181 accaaaaacc aaaaaccaaa aaccaaaaac caagaaccaa gcaccaagaa ccaagaacca
      241 agaaccaaaa accaaaaacc aaaaaccact tcgtcatttg gaactgtcat gggctttcgg
      301 tttcgcaaat ccatcaaaat tgctcccggt ctcaaggtga atctcagcac caaaggcatg
      361 agtgtgaccg ttggtgggca gggcgcttcg gtcaatgtcg gctctgccgg cacatttttg
      421 aatttgggtg tgcctggaac cggtttgtca taccggacaa aggtttcgag caaccgacgc
      481 aagcacccgg cgacgccgcc tccgccaacc ccattgccgc cgccccctgg cacccaacct
      541 ggatggagcc agcagccttc accgccaggt gtccagccaa cactggttcc tgcctcaatt
      601 gggctcaatg ctgactcgcg catctgtttt tataatcaat atggacaggt tgtgcctgat
      661 acctggctcg atcaactggt atctcaaaac tggaaagcag tgaccgaagt gttagagcgc
      721 gaggccgctg cctataatga tgattccgga ttcgacctgc atctggaaac gccttcacct
      781 cgcaaatctt ttgacttacc agaactgatc ctggctggca gtgttccggc cccgcctgag
      841 attttcgatt tttcagagcc gattcccctg ccaccagaac tgaaaacgcc agggtttctg
      901 ggtggattga tgaaatccaa acgcgaccag atcgaagccg aaaacaatcg gctgcgtgaa
      961 gcctatgtcc aggcccagcg cgaatgggaa cgccgccgca ctgcccacac gacctacaaa
     1021 accgaagtga tggctgatta ccaggagcaa ctggccagct acaaacgact caaagccgat
     1081 atcaccgagc gcaatcgggt ccgtcaatca gccttgacca atgggcgcga aggtaacccc
     1141 gaagcgatgg aagatttgct ggccgaagta tttaaagaaa ttgactggtt tcgtgaaaca
     1201 actgccagtt ttgaagtcac atccgacgcc caaaccgtct gggtggatgt tgatttccca
     1261 gaaattgaag acatgccagc ctataccgcc acggttgatt tccctggatt tcggcttaac
     1321 gtgcagccgc gctcaaccta tcgccgccag aaagaataca ttcaccatat tcattccgtt
     1381 gcgtttcgtg tgctgggtga aatcttctgg gcgctaccaa aggtgacatc ggtggttttg
     1441 tctgggtact cgcaacgccc aaatgcccaa accggagtca tggaagatca gtacctgctc
     1501 agtgttcagg tgccacgtgt ggaatggatc aaggttgatt ttttgaactt gaaaggtgtg
     1561 gatgtcttca gcttctttga acagttcaag ttacgacgca agtgttcgcg aacggcaatt
     1621 gcggccccaa tcacgccttt tcgaaccatt gccgatttac atgactggat gccgaccgat
     1681 tgcatgctgt cgcgcaagct cgaactggct gccctgaccc gtgaagaaaa agaatcaatg
     1741 agctggcttg accgacgtca actttcgagc ctcacacctg aagaacttga aatccgtcag
     1801 gtaactgctg aaaaactgtt tggccggttt gttgccgaac gcatcaatat cagccggatt
     1861 gaaaaaggcg atagcgaaga aatcgtcaaa gcgattattg gttcacccac caccactgaa
     1921 gccaaattcc tgcaaggcaa aaaccacgtg acctggaaat atgtcggaag taaacctcat
     1981 cagattaaat ttacggtgaa ttttgaaaac ggcgccgtgg tgagctggga agggaattta
     2041 cctttgggag gctgagaaaa ccggggctga gaaaaccggg gctgaagaaa gcgggctgaa
     2101 gaaagcgggc tgaagactcg caagctcggg gctgaagaaa gcgggct
//
LOCUS       DAOQDO010001115         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49025_length_2145_cov_2.904785, whole genome shotgun sequence.
ACCESSION   DAOQDO010001115 DAOQDO010000000
VERSION     DAOQDO010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49025_length_2145_cov_2.904785"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PKZ53_16435"
     CDS             <1..1004
                     /locus_tag="PKZ53_16435"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ42077.1"
                     /translation="SPSSTTTYAIQSGTDANGNPITGSGSATVTVTSCPEQSSVLVAD
                     TTNNRIQKFNGTTWSVVGPGTVGSGNGQFRVPEAVAFNTSGRIYVADTGNNRIQWSTN
                     GGTTWATFAASGTGLNQVSGPRGLALDSIGNLYVADGGNGRVVRFNNGVPGNAVILAT
                     KGTTAGRVQTPNGLAIDISFNLFIADTGNNRIQKITSAHTKTTPNTGTQIAGLGSGLT
                     AVRAPQGVAVDSSGNLYVADTGNNRITRFAGGNPGTATLLAGAGTALGQVRAPEGVTL
                     SAFSTGPLVGGISLVVSDTTNNRIQGRNLNGTTWVLVGTPNGLGTNPGQFRAPSKLR"
     gene            1136..1573
                     /locus_tag="PKZ53_16440"
     CDS             1136..1573
                     /locus_tag="PKZ53_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42078.1"
                     /translation="MSYTVFIFFGEQNLENLSAAVHLTETIMGFHFSGAEKNQARLLD
                     ISLEVCQRSSFLCHTEDMPFGDFQYQLELSTPVFRGELIHPIGLYLTREFSSRLNQRS
                     MLCLAGVEFLGAMFEKGRLVTDRMGLLPHLHACGPWQYCPESK"
     gene            complement(1714..2022)
                     /locus_tag="PKZ53_16445"
     CDS             complement(1714..2022)
                     /locus_tag="PKZ53_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42079.1"
                     /translation="MQEWEWHLLEDRTAVPQTVVQGVELKPGDRVWLRPQRGGDIFDL
                     VLAGKLAVIEAIEQDYENKVHVAVVVEDDPGQDLGHQRLPGHRFFFSIEEIEPWLEEQ
                     "
BASE COUNT          500 a          562 c          545 g          538 t
ORIGIN      
        1 tatcgccttc cagcaccaca acctatgcca ttcaatctgg aacggatgcc aacggaaacc
       61 cgattactgg cagcggaagc gcaaccgtga cagttacatc ttgtccagaa cagtcatccg
      121 ttttggtggc cgacacaacc aataatcgta tccagaagtt taatggaaca acctggtcag
      181 tcgttgggcc tggaacagtt ggatctggaa acggtcaatt ccgggttcct gaagccgttg
      241 cctttaacac cagcgggcga atttatgtgg ctgacactgg caacaatcgc attcagtggt
      301 caaccaatgg cggcacaacc tgggcaacct ttgccgcgag tgggacggga ctgaaccagg
      361 tgagcggacc acgcggtctg gcgcttgaca gtatcggcaa tttgtatgtc gcagatggtg
      421 gaaatggtcg tgtggtgcgt ttcaacaacg gcgtacctgg aaatgccgtt attttggcca
      481 cgaaaggcac caccgccgga cgggttcaaa cgccaaatgg gctggcaatt gacataagct
      541 tcaatttgtt tattgccgac accggaaata accggatcca aaaaatcacc agtgcccaca
      601 caaaaaccac acctaatact ggaacacaga ttgccgggct cgggtctgga ctgacagcgg
      661 ttcgggcgcc acaaggcgtg gccgttgatt cctctggtaa tctctatgtt gctgacacgg
      721 gaaataatcg catcacccgg tttgcaggag gtaatcccgg aactgcaacg ctgcttgccg
      781 gagcgggcac ggcgcttgga caggtcagag cgccggaagg tgtcaccctc agtgcttttt
      841 ccactggacc actcgttggc ggaatttcac tggttgtcag cgataccacc aacaatcgaa
      901 ttcaaggccg caatttaaac ggcacgacct gggtcctggt tgggactcca aatgggctcg
      961 gcactaatcc aggtcaattc cgcgccccat caaagctgag ataacgcaat aatgatgtgg
     1021 ttgcttatct gacaatcggt ttcctctcct ttatgtaacc tgcctggact actccggttc
     1081 tggcaggttt tcttttttct gaagaagagg tttgaaaatt tgggtagggt acttgatgag
     1141 ttatactgtt tttatctttt tcggagagca aaacctggag aatctatcag cggctgtcca
     1201 tttaaccgaa accatcatgg gtttccactt ttcaggggca gaaaagaatc aagcccggct
     1261 gctggatatt tcgcttgaag tttgtcaaag gtcttcgttt ctttgtcata ctgaagatat
     1321 gccttttggt gatttccagt atcaactgga actttccact cccgtttttc gaggtgaact
     1381 cattcatcca attggcctct acctgacgcg tgagttctca tcacggctca accagcgctc
     1441 catgctgtgt ctggcgggcg tcgagttcct tggcgccatg tttgaaaaag ggcgacttgt
     1501 aactgatcgg atgggtcttt taccacactt acacgcttgc ggcccctggc aatattgccc
     1561 tgagagcaaa tgattctgct gctgccggat tttgtgtcag gtcaggtcag tgttggaaat
     1621 aaatgacttg tcagtaccac tcattgaggc aggtggtact cactcatcgg ttcttctggt
     1681 ttatctccaa cacaaaatcc gctagaaaca ggttcattgt tcttccagcc agggttcgat
     1741 ttcttctatc gaaaaaaaga accgatggcc ggggagccgc tggtggccga gatcctggcc
     1801 agggtcatcc tccacaacca ctgccacgtg gaccttgttt tcatagtcct gctcaatggc
     1861 ctcgatcacg gcgagttttc cagccagaac caggtcaaaa atgtcgccgc cccgctgcgg
     1921 acgcagccag acacggtctc caggtttgag ttcaactccc tggacaacgg tttggggcac
     1981 tgccgttcga tcttccaata agtgccattc ccattcctgc ataaggctca aggctccatt
     2041 gggcgaagtc cacgcagggc accgtggagc ttcatcaaat gttgttccgg aagagtttcg
     2101 gttcgttcca aaatgcggcg cgcccgatca tcaacgcctc gcatt
//
LOCUS       DAOQDO010001116         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49026_length_2145_cov_2.888517, whole genome shotgun sequence.
ACCESSION   DAOQDO010001116 DAOQDO010000000
VERSION     DAOQDO010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49026_length_2145_cov_2.888517"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="PKZ53_16450"
     CDS             complement(<1..1843)
                     /locus_tag="PKZ53_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNJ42080.1"
                     /translation="MYAKIAIDPTRINARETDVFDIFNVRHYIGPNPYLETEALVFDF
                     ALTGDPSPASIQEFLRDIARELPQLKEEIFDSHAHLFARTVSEVGKLNIGLHLNQWSL
                     KTYPEFVRVAVQCLHKSTQWEVLYCVWDWFEAIGARQSFNFQERLKGLQEMFSRSVFG
                     GPSTYALIRAAQQRGIPTTYLWNEGLVQYGYGKKQIRGASTTFSTDSHLDSDFTCRKD
                     DCKAFLGEMGFPVPQGSVVATFAEAAQLAREVGYPVVVKPLAGHKGIGVTANILNETE
                     LEFAFRKAVESIETEGGPVDVIVEKNITGSDFRLLCIHGKFTAAVKRQPASVVGDGNS
                     TLQMLIDRENQSPARQDTPTSPLGKIKSDDSMMMFLQEQGLTLESVIEPGRRIFLRKV
                     ANLSAGGVSYNVTDTVHPDNVTLAEDIAQYLHLTCLGIDVIAGDLATSWKEGGFGIIE
                     INAAPGIFMHLNPAGGSSIDVPSKIVESFFPTPESATIPIITLNRATRNSVRNLTAAL
                     LNQNPTLTIGAVCQSAVVVNQREKFKHPEYNHNVSSLLRNPKLDVLIAEYRDEILEAE
                     GMVYQSSTVVILENPTETERTLARDVCENGLLIVLQGRNVTVRRQGRT"
     gene            complement(1896..>2145)
                     /locus_tag="PKZ53_16455"
     CDS             complement(1896..>2145)
                     /locus_tag="PKZ53_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42081.1"
                     /translation="HNLKITLVSPGETIDLTTGVVFQPVVSKDHPINIHTILSHLARD
                     CGATLTTTDQDPLPPIHFNMVPTDNPQSALLSVMSLRG"
BASE COUNT          539 a          528 c          540 g          538 t
ORIGIN      
        1 gggttctgcc ctggcgccga acggttacat tccggccctg gagcacaatc agcagaccat
       61 tttcgcaaac atcacgggcc agtgtccgtt cagtttcggt cggattttca aggatgacca
      121 cggtactgga ttgatagacc atgccctcgg cttccagaat ttcatctcga tattcagcga
      181 tcagtacatc gagctttgga tttcgcaaca agctggaaac attgtggtta tattcaggat
      241 gtttgaattt ctccctctgg ttgaccacca cggctgactg acaaaccgca cctatggtca
      301 acgttgggtt ctgattgagc aaggctgcgg tcaggttccg aacgctgttt cgggttgccc
      361 gattcaacgt gatgatggga atggtggctg attcaggggt tggaaagaaa gattccacaa
      421 ttttggaggg cacatcaatg cttgagccgc cggctggatt caaatgcatg aagattcccg
      481 gtgcggcatt gatttcaatg atgccaaagc caccttcctt ccacgacgta gccagatcac
      541 ctgcaatcac gtcaatgcca aggcaggtca ggtgcaaata ctgggcgatg tcttccgcca
      601 gggtgacatt gtcagggtga accgtatcgg tgacgttata actcacccca cctgccgaca
      661 aattggccac tttgcgcaga aaaatgcgtc gtccgggttc gatgacgctc tcaagtgtca
      721 gtccctgctc ctgtaaaaac atcatcatgg aatcatcaga tttgattttc ccaaggggtg
      781 aagttggcgt gtcttgccgg gctggggact ggttttcacg gtcaatcaac atttgcaagg
      841 ttgaatttcc atctcccaca accgaggccg gttgacgttt aacggcggca gtaaactttc
      901 catgaataca cagcaatcga aaatctgacc cggtgatatt cttctcaacg atcacatcca
      961 cggggccacc ttcggtttcg atagattcaa cggctttgcg aaaagcaaat tccagttcgg
     1021 tttcattgag aatattggct gttacgccaa ttcctttatg gccggccagt ggtttgacaa
     1081 caactggata cccgacttcg cgggcaagct gggcggcttc tgcaaaggtt gccaccacac
     1141 tgccctgggg gactggaaat cccatttcgc ctaaaaatgc cttgcaatca tctttgcgac
     1201 aggtgaagtc agaatccaga tggctatcag tcgaaaaggt tgttgaggca ccccgaattt
     1261 gttttttacc atatccgtat tgaaccagtc cttcattcca gaggtaggtg gttggaatgc
     1321 ctcgctgctg ggcggctcga attaacgcat aggttgaggg gccaccaaac accgaacggc
     1381 taaacatttc ctgaagaccc tttaaccgtt cctgaaaatt gaaagattgg cgagccccaa
     1441 tggcttcaaa ccagtcccac acgcaataca ggacttccca ctgagttgat ttatgcagac
     1501 actgaactgc cactctgaca aactccgggt aggtctttaa actccactga ttcaggtgca
     1561 ggccgatatt gagcttgccg acttccgaaa cggttcgggc aaagaggtga gcgtgggaat
     1621 caaagatctc ctctttgagt tgaggcaatt cgcgcgcaat gtcccgtaaa aattcctgaa
     1681 ttgaagccgg tgacgggtct ccggtcagag caaagtcaaa taccagcgct tcggtttcaa
     1741 ggtacggatt gggtccgata tagtgacgga cattaaaaat atcaaacaca tcagtctccc
     1801 gtgcattgat ccgcgtggga tcgatagcaa ttttggcgta catacaaatt caaactccag
     1861 ttgtaggcga agataccctc atcccgaatt ggtatttatc ctcgcaggct cataacactg
     1921 agcaaggccg actgcgggtt gtcagtcgga accatattaa aatgaatggg tggcaatgga
     1981 tcctggtcag tcgtggtcag cgtggcacca cagtcacgcg ccagatgcga aagaattgtg
     2041 tgaatattga ttggatggtc tttggaaacc actggttgaa acaccactcc agtggtcaaa
     2101 tcaatcgttt caccaggact gaccagcgta attttgaggt tgtgg
//
LOCUS       DAOQDO010001117         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49067_length_2144_cov_2.998085, whole genome shotgun sequence.
ACCESSION   DAOQDO010001117 DAOQDO010000000
VERSION     DAOQDO010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49067_length_2144_cov_2.998085"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..>2144
                     /locus_tag="PKZ53_16460"
     CDS             101..>2144
                     /locus_tag="PKZ53_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42082.1"
                     /translation="MSSMIPFTNNHSLTRLITDLTVDQDLESSVGHITRLVTQLLKIP
                     LATLCLGEPPQYWFMGTYGFQLSATSQTSGLWSHAFSVRQPVIIEDTHQTPAVAMDEL
                     VVQPGGIRFFAAVPIYVQHLGYFAALCLADTTPRTLAETDLHWFQEFAGLIHDQLNLA
                     MTTLSQSQQSLQFDVLLQRFSDFILILETDGVVRYQSPGVEKLIGYASSQVAGQSVFD
                     FVHPDDQQTVQDLLTEVLEQTDFTISTEHRLKTQAGDWIWVESLVHNLLRDPHISGIL
                     ISSRDITERKEAESALQANEAKYRALFNAIPDLIFQLNRDGILLGHKASKLETLVLPD
                     KILGRHITEVFPRELATQVLQLIDETFRHSVTTLFEYQLLTGNQVREFEARMVVSGPD
                     EVLVMVRDITERKGLEREQATAREAAIEAARMKSEFLANMSHEIRTPMNGVVGMTGLL
                     LDTNLDEEQHEYTRIIRQACDSLLNIINDILDFSKIDVGKLRLEEIEFELWPVIEDVI
                     RFHLELAQLRELELTFQIDARVPKIVVGDPGRLRQILTNLIGNAIKFTEQGTITVQVT
                     LADHQPFFSIPSSERTMLQTAELYPTWIRFEVTDTGIGIPAHLQGRLFKPFSQVDSSM
                     TRRYGGTGLGLVICKQLVQLMGGEIGVESHAAQGSRFWFEVRLDIPKNPMRELISKT"
BASE COUNT          522 a          591 c          506 g          525 t
ORIGIN      
        1 acttcttcag cccaacttct tcagcccaac ttcttcagcc caacttcttc agccctcagc
       61 ccctagctca atttcttcag ccccaatctg ggatgacact atgtcctcaa tgattccatt
      121 taccaacaat cattctctca ccagattgat cactgatctc accgtagatc aggacctgga
      181 atcttcggtt ggacatatca cccgactggt gacccaactc ctcaaaatcc ccctggccac
      241 gttgtgttta ggcgaaccac ctcagtactg gtttatgggg acgtatggct ttcaactatc
      301 agcgaccagc caaacatccg gcctctggag ccatgccttt tcagttcgac agccagttat
      361 tattgaagat acccaccaga cacctgcggt ggcgatggat gagctggtgg tacagcccgg
      421 tggaattcgc ttttttgccg ctgtcccgat ttatgtgcag catcttggct attttgccgc
      481 cctgtgcctg gccgatacga cgccccgcac attggccgaa actgacctcc actggtttca
      541 ggaatttgcc gggctgatcc acgatcaact caatctggcg atgacaaccc tgagccagag
      601 ccagcaatca ttgcaatttg atgtgctgct gcaacgcttt tctgatttta ttctgatcct
      661 tgaaactgat ggggtggttc gctatcaaag cccaggtgtt gagaaactga ttgggtatgc
      721 gagcagtcag gtggctggtc agtcggtctt tgactttgtg catcctgacg atcaacaaac
      781 cgttcaggac ctcctcactg aggttttgga acagaccgat tttacgattt ccaccgagca
      841 tcgcttaaaa acccaggccg gagactggat ttgggttgaa tcactggtgc ataacctgct
      901 gagagaccca cacatcagcg gcattttgat cagttctcgc gacatcaccg aacgcaaaga
      961 agctgaatcc gccctccaag caaatgaagc caaataccgg gccctgttta atgccatccc
     1021 cgatctgatt tttcagctta atcgggatgg gattttgctt ggccacaaag cctcaaaatt
     1081 agagacactc gtcttgccgg ataaaatcct cggacgccat atcaccgaag tcttccccag
     1141 agaactggcc acccaggttc tacagttaat tgacgaaaca tttcggcatt cggttaccac
     1201 cctgtttgaa tatcaattgc tcaccggaaa tcaggttcgc gaatttgaag cccgcatggt
     1261 ggtctctgga ccagatgaag tcctcgtcat ggtgcgcgac atcactgagc gcaaagggtt
     1321 ggagcgcgaa caggcgacag cgcgtgaagc cgccatcgaa gccgcccgca tgaagagcga
     1381 atttctggca aatatgagcc acgaaatccg caccccaatg aatggcgtgg tcggaatgac
     1441 tggcctgttg ctcgacacca acctggatga agaacaacac gaatacaccc gcatcattcg
     1501 gcaggcgtgc gattccctgc ttaatatcat caacgacatt ctcgattttt cgaaaattga
     1561 tgtcgggaaa ctccgtcttg aggagatcga gtttgagttg tggccggtaa ttgaagatgt
     1621 gatccgcttt catcttgaac tggctcaact gagagaactc gaattaacct ttcaaattga
     1681 cgcccgggta cccaagattg tggttgggga tccgggtcgc ttgcgacaaa tcctgaccaa
     1741 tctgatcggc aacgccatca aatttactga acaggggacg attacggttc aggttaccct
     1801 ggccgaccac cagccgtttt tcagcattcc ctccagcgaa cgcaccatgc tccagacggc
     1861 tgagctttat ccgacctgga tccggtttga agtcaccgat actggcatcg ggattccagc
     1921 ccacctgcaa ggccggttgt ttaaaccatt ttcccaggtt gacagctcaa tgacccgccg
     1981 ctacggaggc actggacttg gtctggtcat ctgcaaacaa ctggttcaat taatgggggg
     2041 agaaattggc gttgaaagtc acgcggccca gggatcacgg ttctggtttg aagtcagact
     2101 cgatatcccc aaaaatccaa tgcgggaact gatttcaaag accc
//
LOCUS       DAOQDO010001118         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49074_length_2144_cov_2.718526, whole genome shotgun sequence.
ACCESSION   DAOQDO010001118 DAOQDO010000000
VERSION     DAOQDO010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49074_length_2144_cov_2.718526"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKZ53_16465"
     CDS             <1..309
                     /locus_tag="PKZ53_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ42083.1"
                     /translation="RVNAIAPGPFPTPGAWEKLMPSPEFEAEAKRRNPSRRFGEHIEL
                     ANLAAYLVSPYSDYINGECVVIDGGEWLMGGEFNHLATLPPEMLAAMASKIRKPKKDG
                     "
     gene            491..892
                     /locus_tag="PKZ53_16470"
     CDS             491..892
                     /locus_tag="PKZ53_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42084.1"
                     /translation="MKYNVGGEVDTYCGKCKLERTHKIVAVDPDGSIVKVVCATCESY
                     HKYKAPKVVKERSAKVSKSTKKDKPAQIDFSMIEMMTKSAVPYSVRTLYQIGDVIDHT
                     TFGLGQVVAIEEDKIDVHFGVGIKKLVHGRN"
     gene            complement(1044..2006)
                     /locus_tag="PKZ53_16475"
     CDS             complement(1044..2006)
                     /locus_tag="PKZ53_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNJ42085.1"
                     /translation="MKKLLLPEKGIEVLFGSYDENIKYLETLLNIRVGMRGSDLMVEG
                     SDEDTRLVEQILEDFISLMNEGVTPGSDELKTAFKQIADDRTMTLRTYFTKTVRFNPT
                     GKKMVSARSTNQRRYLEAIDANDIVFGIGPAGTGKTFLAVAMALQALWNKRVTRIILT
                     RPAVEAGERLGFLPGDLQDKVDPYLRPLSDALFDLAEYDKIAKMLEKRIIEIAPLAFM
                     RGRTLSDAFIILDEAQNTTSEQMKMFLTRIGHSSRAIITGDVTQIDLPFGKTSGLIQV
                     QQILEGIEGISFIKFTHRDVVRHRLVQMIIQAYDRHTKLTQDPV"
BASE COUNT          569 a          523 c          509 g          543 t
ORIGIN      
        1 agagtcaatg ccattgcccc agggcccttc cccacgcctg gggcctggga aaaattgatg
       61 cccagtccag aatttgaagc cgaggccaag cgccgcaacc cttctcgccg ctttggtgaa
      121 cacattgaac tggccaatct ggctgcgtat ctggtttctc cctatagcga ttacatcaat
      181 ggtgaatgcg tggtgattga tggtggcgaa tggctcatgg gtggcgaatt taaccatctg
      241 gcaacgctgc cacctgaaat gctggcagcg atggcttcaa aaatccgaaa acccaaaaaa
      301 gatggataac tgatatcaac cacacggagg tgctggccgc caaccagaac gcattttgtt
      361 gaagactggt ttatcccaaa ccggacagca ccaatccaac cagccggaag cacgttcatt
      421 gtttccgaaa caggtagtaa tcattcaact taaacacatt cctgggacaa ggggaaaata
      481 agaaaaactt atgaaataca acgttggtgg cgaagtcgat acttattgcg gaaagtgcaa
      541 acttgagcgg actcacaaaa ttgtggcggt tgatccagat ggttcaattg taaaagtcgt
      601 ctgtgcaacc tgtgagtcat accacaaata taaagcaccc aaggttgtaa aagaacggtc
      661 agcgaaagtt tccaaatcca ccaagaaaga caaacccgct caaattgatt tttccatgat
      721 tgagatgatg accaaatctg cggtgccata ttcggtcaga accctgtatc aaattggaga
      781 tgtcattgat catacaacct ttggtttagg ccaggtggtg gctatcgaag aagataaaat
      841 cgatgtccac tttggggttg gcatcaaaaa actggtgcat ggccgaaact gaagacaacg
      901 ggctgaaaaa ccttgggctt cagggctgat ggctgaagaa aatgggctga gggttgaatt
      961 tccatttttt tcaaccctca gcccaatgtt ttcagccccg agtttgcgag ctttcagtcc
     1021 gttttcgttc gcccaatggt gtttcaaacc ggatcctgtg tcagtttggt atgccggtca
     1081 taggcttgaa tgatcatttg gaccagtcgg tggcgcacca cgtcgcggtg ggtaaattta
     1141 ataaatgaga tgccttcgat tccctccagg atctgctgaa cctggatcag ccctgacgtt
     1201 tttccgaagg gaaggtcaat ttgggtcacg tcacccgtaa tgatcgcccg tgaactgtga
     1261 ccaatccgag tcaaaaacat tttcatttgc tctgaagtcg tattctgcgc ttcatccaaa
     1321 atgataaatg catctgacag ggttcgaccc cgcataaaag ccagcggggc aatctcgata
     1381 attcgctttt ccagcatctt ggcaatttta tcgtattcag ccaggtcaaa cagggcatca
     1441 gagagtggcc ggagataggg atccacctta tcctgcaaat ctccaggcaa aaagccaagc
     1501 cgttccccgg cttcaaccgc tggccgggtt aaaataatgc gggtgacccg tttgttccac
     1561 agcgcctgca acgccatggc caccgccaaa aaggtcttcc ctgttccggc tggaccaatt
     1621 ccaaatacaa tatcattggc atcaatcgct tccagatagc gtcgctggtt ggtagaccgg
     1681 gctgacacca ttttcttccc ggtcgggtta aatcgtaccg ttttggtgaa ataggtccgc
     1741 agcgtcatgg ttcgatcatc ggcaatctgt ttaaaagccg ttttcagttc gtcgctgcca
     1801 ggtgtgaccc cttcattcat caaactgatg aagtcttcca gaatctgttc gaccagccgt
     1861 gtatcttcgt cgcttccctc caccatcagg tcgctgccac gcattccaac acggatattg
     1921 agtaaggtct caagatactt gatgttttca tcataggaac caaacaaaac ttcgatgcct
     1981 ttttcaggaa ggagcaattt cttcaattga atttcctcac aaaacccttg aatcaagggg
     2041 gttgggggtt aatgttccgg taccgggatg gcgttaccga tgatcaaaat ctcagaactg
     2101 atggggtgct gagaagtcgg atcttctggc atcttttccc aaat
//
LOCUS       DAOQDO010001119         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49083_length_2144_cov_2.308760, whole genome shotgun sequence.
ACCESSION   DAOQDO010001119 DAOQDO010000000
VERSION     DAOQDO010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49083_length_2144_cov_2.308760"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="mobF"
                     /locus_tag="PKZ53_16480"
     CDS             complement(<1..1011)
                     /gene="mobF"
                     /locus_tag="PKZ53_16480"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HNJ42086.1"
                     /translation="MLKICGPLAAHQTTTYYQKEYSRGDYYIGSEEQPIAGIWLGKGA
                     ASLGVQGQVEHHDFAKLADGISPDGKQLIRHSPGKNRSEHRSAWDCTFSAGKSVSIVA
                     LAEGGDRGLIDDHHAACEVAFGVLEEYAQARAGADRNQIHHTKNLVAARFTHLENRRL
                     DPQLHSHHVVFNLTYDDQALSRNGEVPGGWRALQTRGMMQAQELATSVYRAELAHRIQ
                     ARGYDIYLDCYGAVQLTGVPEALLLACSTRRAEIEVEAAARMAKAEARARAEGRTLTD
                     RERGRIQEMATLHTRVAKQKNISPTELRAGWKLRYETFGFDVAAYVSQIRHKANKNLF
                     SSL"
     gene            complement(1023..>2144)
                     /locus_tag="PKZ53_16485"
     CDS             complement(1023..>2144)
                     /locus_tag="PKZ53_16485"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNJ42087.1"
                     /translation="DIWLYPPDKRCPYWDLEAEIETEDDAEALARAFLPPENEVNPTF
                     WDMAKTRLFKFLLIRMKMERCGVAGLLEWLRDENKIDALIQAYDETLKPLIKENAAPQ
                     RAGVFGALSAVADSLKKLPPNDGRPRFSFRQWAKTGKNWVFIGSNFGSQDVLRPVIAA
                     MIQTMCGALMRDKNAPRTFFFLDEIPSLGKLVSLKNALQRAGKFNLIFILGFQGRAQL
                     EHIYGKEAETLMSAPGIRIFLKTKEYGAAEWCANNAGKPEQERQVESVTSGEGENRGS
                     RTYNLERKIQHLITPNEFQNLEKLHGYLRYDKYLCEIRFGYPNLPQINSVEVRPEKIL
                     SPHHPIQKAMPVGQNADQKPKPSTPPLKKEASEDLDFRV"
BASE COUNT          454 a          573 c          534 g          583 t
ORIGIN      
        1 cagtgaggaa aataggtttt tgttggcttt gtgccggatc tggctcacat aggcagccac
       61 atcaaaccca aacgtttcat agcgcaattt ccacccggcg cgaagctccg tgggactgat
      121 gtttttctgt ttggcgaccc gcgtatggag cgttgccatt tcctgaattc gccctcgctc
      181 ccgatcagtg agcgttcgtc cttcagctct ggccctggcc tcggcctttg ccattcgagc
      241 ggcggcttca acctcgattt ccgcccggcg ggttgaacag gcgaggagta aggcttccgg
      301 cactcccgtc agttgcacgg caccgtagca gtcaagatag atgtcatacc ctctggcctg
      361 gattcggtgt gccagttcgg cgcggtacac gctggttgcc agttcctgag cctgcatcat
      421 tccacgggtc tggagcgcgc gccacccgcc tggcacttca ccatttcggc tgagtgcctg
      481 gtcatcatag gtcagattaa aaaccacatg atggctatga agttgggggt cgagccgccg
      541 gttttcaaga tgagtaaagc gtgcggcaac caggtttttc gtgtgatgaa tttgattgcg
      601 atcagcgccg gcgcgggcct gggcgtattc ctccaaaacg ccgaatgcca cctcgcaggc
      661 ggcgtggtga tcgtcaatca ggccacgatc acctccctcc gccagagcta caattgatac
      721 tgatttcccg gctgaaaacg tgcagtccca ggcactgcgg tgttcggacc ggttcttccc
      781 tggagaatgt cgaatcaact gttttccatc cggggaaatg ccatcagcca gtttggcaaa
      841 atcatggtgt tcaacctgcc cctgcacccc taaggaagct gctccttttc caagccaaat
      901 accagcaatg ggttgttcct cagacccaat gtaatagtct ccccgggaat attctttttg
      961 gtaataggtt gttgtctgat gagccgccaa tggaccacaa attttcagca tttatccccc
     1021 ctttacactc ggaaatccaa atcctcagag gcttcttttt tgagaggtgg agttgatggt
     1081 tttggtttct ggtcggcgtt ttgaccgaca ggcatcgctt tttgaattgg gtggtgcggc
     1141 gaaaggattt tttcgggtcg aacttcgacg gaattgattt gtggcaggtt cggataccca
     1201 aacctgatct cgcataaata tttatcgtac cgcaggtaac catggagctt ttcgaggttt
     1261 tgaaactcgt ttggcgtaat caaatgctgg atttttcgtt ccaggttata ggtccgactt
     1321 ccccggttct cgccttcacc gctggttacg ctttcaacct gacgttcctg ttccggtttg
     1381 ccggcattgt tggcacacca ttcagccgca ccgtactcct tcgttttcag gaaaattcga
     1441 attcccggtg ccgacatcag cgtttcagcc tctttgccgt agatatgttc aagttgagcc
     1501 cgtccctgaa atcccagaat aaatatcagg ttgaatttcc cggcccgttg aagggcattt
     1561 ttcaacgata ccagtttccc aagcgatgga atttcatcga gaaagaaaaa tgttcgcggt
     1621 gcatttttat ctcgcatcag tgcgccacac atggtctgga tcatggctgc aatcaccggc
     1681 cttagaacat cctggcttcc aaaattactg ccaatgaata cccaattttt tccagttttt
     1741 gcccactgac gaaatgaaaa ccgtggccgt ccatcattgg gtggaagctt tttgagcgaa
     1801 tccgccacgg ctgagagcgc cccaaaaaca cctgctcgtt ggggtgcggc attttcttta
     1861 atcagaggtt tgagtgtttc atcatatgcc tgaatcaacg catcaatttt gttctcatcc
     1921 ctgagccatt caagcagtcc ggcgactcca catcgctcca ttttcatgcg aataagcagg
     1981 aatttaaaaa gccgggtttt ggccatatcc cagaaggtgg ggttgacttc attttcgggt
     2041 ggcaggaatg cccgagcgag cgcttccgca tcgtcttccg tctcgatttc agcttccaaa
     2101 tcccagtatg gacaccgttt gtctggagga tagagccaga tatc
//
LOCUS       DAOQDO010001120         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49223_length_2140_cov_3.007674, whole genome shotgun sequence.
ACCESSION   DAOQDO010001120 DAOQDO010000000
VERSION     DAOQDO010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49223_length_2140_cov_3.007674"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKZ53_16490"
     CDS             complement(<1..748)
                     /locus_tag="PKZ53_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42088.1"
                     /translation="MRSLWLVMGCVVWLWSLPCLAQNPIPAPVSKEIKTVTKTVVIRK
                     NVIFKSLDVIADRKDFLVEIDGKPRELQEKSFRWQTSDLFKVGTQVNVRAELPGELRD
                     VKTIKIEQDDTTTVYYGWADAQLTVLTDRPGVEIELEQDELSKAISGDKGYPISSDEK
                     KKIEYGYLTPGLIKIKAKFPALPDLVKVVELKSDIPSTVDIGWNDSEIRILGVEKIYP
                     RLSFLVNGQPMKGEYNESRKSMVIRGLPAGH"
     gene            complement(745..>2140)
                     /locus_tag="PKZ53_16495"
     CDS             complement(745..>2140)
                     /locus_tag="PKZ53_16495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ42089.1"
                     /translation="KPIDTALRLWLQILEGLDQIHSEGVMHLDLKPENVLLTSRDAQS
                     AEVKITDYGLAELVGIIRQSGGTPAYMSPESLSQAQPSILSDLWAVAVIGYELLTGTV
                     PFSVRSAKLTVSELESAFFKAEVWLPEELSPFRAFFDRAFHRSVYDRFQSAADMVNQI
                     ELIRSEFAGPALQVPVMPDRGSFPGRILSSALFSRLRRQPHGHPISTAASTWKMFDQT
                     SRSKMLATMALTGALLAGGGWIGWKFLAPKWMLTPPPTTLPSAPIAPPGMVYLHPGNG
                     KQAFFLDRFEVTNGDYDSFLGSDAARRQDGYEDRRIRWELHKQLLLTWQATNPKDDWQ
                     RYPVTNVTWFDAMAFAQARGRRLPTSEEMEAASLGVKTNDTLGQSGPLHVDHRCSASN
                     PFGIIGLSCNVSEWTSTSYSDQASSDQKLIIGGNWQNETTLMYAPPTRQEGYIGFRCA
                     ADPPPPGQKGSEQR"
BASE COUNT          520 a          549 c          543 g          528 t
ORIGIN      
        1 gatgtccagc cggcaaacct cgaatgacca tagatttgcg cgattcgtta tattcgcctt
       61 tcatcggctg gccgttgacc agaaatgaaa gtcggggata gatcttttct acgccaagga
      121 tgcgaatttc gctgtcattc cacccaatat caaccgttga cggtatatcc gattttaatt
      181 caaccacttt caccaaatcc ggcagggccg ggaatttcgc tttgattttg atcaggcccg
      241 gcgtcagata gccatattcg atctttttct tctcatcact gctgattggg taccctttat
      301 cgccactgat ggccttgctc aattcatctt gttccagttc gatttcaacg cctggacggt
      361 cagtgagaac cgtgagttgc gcatcggccc agccatagta gaccgtggtc gtgtcatctt
      421 gttcgatttt gatggttttg acgtcgcgta gctcacctgg gagttcggcg cgaacattga
      481 cttgagtgcc aaccttaaat aaatcagatg tttgccagcg aaaacttttt tcctgcaatt
      541 cacgcggctt gccatcaatt tcaaccagaa aatccttgcg gtcagcaatg acatccaggc
      601 ttttaaaaat gacattcttg cgaatgacaa ctgttttcgt gacggttttg atttctttgg
      661 aaactggtgc gggaattgga ttttgagcca gacacgggag cgaccacagc cacaccacgc
      721 accccataac caaccacaat gatctcatcg ctgttcagaa cctttctgcc caggtggtgg
      781 agggtcagca gcacagcgaa acccgatata gccttcctgt cgagtcggag gggcatacat
      841 caacgttgtt tcgttttgcc agtttccacc aataatcaat ttttgatcgg atgaagcttg
      901 atcagaataa ctcgtgctag tccactctga aacattacaa gaaaggccga tgattccaaa
      961 gggattcgag gcggaacagc gatgatcaac gtgcaacgga ccgctttggc ccagggtatc
     1021 gttggttttt acccccagag atgccgcttc catttcttca ctggtcggca accgccgtcc
     1081 acgtgcctga gcaaaagcca tggcatcaaa ccaggtgaca ttggtgactg gatagcgctg
     1141 ccagtcgtct tttgggttgg ttgcctgcca ggtcagcaag agttgtttat gcaattccca
     1201 gcggatgcgg cgatcttcat agccatcctg gcgtctggcg gcgtcacttc ccaaaaacga
     1261 gtcatagtca ccgttggtga cttcaaagcg atccagaaaa aatgcctgtt ttccattgcc
     1321 tgggtgaaga tagaccatgc ctggtggcgc tattggagca gacgggagcg tggttggagg
     1381 cggggtcaac atccatttgg gggcaaggaa cttccatccg atccagcccc cgccagccag
     1441 caaggcgcca gtgagagcca ttgtggccag catttttgat cgcgaagtct ggtcaaacat
     1501 cttccaggtc gaagctgctg ttgagatcgg atggccgtgc ggttgtcgcc gcaatcggga
     1561 aaacagtgcc gatgataaaa tccggcctgg aaatgacccg cgatccggca tgaccggcac
     1621 ctggagcgct ggaccggcaa attctgaccg gatgagttca atctggttga ccatgtcggc
     1681 ggctgattgg aaccgatcat agacggagcg gtgaaaggcc cgatcaaaaa aagcgcggaa
     1741 aggactcaat tcttcgggaa gccagacttc agctttaaaa aaagccgatt ccagttctga
     1801 aaccgtcaat ttggcagatc ggaccgaaaa cgggacggtc ccggtgagca attcataccc
     1861 aatcaccgcc accgcccaca aatcagagag aattgacggc tgggcctgag acagtgattc
     1921 cggcgacata taggctggcg ttcctcccga ctgacggata attccaacca gttcggccag
     1981 accatagtcg gtaattttga cttcggctga ctgggcatcc cgactggtca acaggacatt
     2041 ttccggcttt aaatcaagat gcatgactcc ttcggaatga atttgatcaa gtccttccag
     2101 aatctgaagc cataacctga gtgcggtatc aattggtttg
//
LOCUS       DAOQDO010001121         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49312_length_2138_cov_2.889102, whole genome shotgun sequence.
ACCESSION   DAOQDO010001121 DAOQDO010000000
VERSION     DAOQDO010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49312_length_2138_cov_2.889102"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..637)
                     /locus_tag="PKZ53_16500"
     CDS             complement(53..637)
                     /locus_tag="PKZ53_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNJ42090.1"
                     /translation="MTPQIEQVASRLLAEDRRKLTEIEKRFIRRLAERRLISANINQQ
                     FDEQLTFGQRVADQIASFGGSWPFIFLFFFTLLFWMALNSLVLKDKAFDQYPYILLNL
                     ALSCLAALQAPVIMMSQNRQAAKDRLQAEQDYQVNLKSEFEISRLHEKVDALHAAYVA
                     ELKQVEERHTILLEEILALLEDQQGEDSRQPANK"
     gene            complement(769..1908)
                     /gene="lpxK"
                     /locus_tag="PKZ53_16505"
     CDS             complement(769..1908)
                     /gene="lpxK"
                     /locus_tag="PKZ53_16505"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNJ42091.1"
                     /translation="MPETEADATRIQVPGWGNIPRSWLYVPGKLYEFGVRLRLALYET
                     GYLKPKALSNPVISVGNITLGGTGKTPLVEFIARYLTGEGHEVAILTRGYARQDRRQP
                     RVVVSDGTQICATLAEAGDEPLMLARRLSGVKVVVGKNRHANGTWLEANQACEVHLLD
                     DGFQHVQLQRDLNLLVLDATDPFGGGEMVPFGRLREPLYALKRADAIIVTRADQAFDE
                     DELRWILNECGVDVPLIFSFHEVTGVYQLQDGKPQAQRSLAGKAVGVVSALGNPDRFE
                     HDVATLGAKIVFRKHLRDHHGYSQKDVSEIVSAAQLAGAERLVTTEKDAVKLEQFHFG
                     SLPVSVLEIKARFDDEVSLKTLLLRTIVRKQKSKRVEDSGRKTSG"
BASE COUNT          519 a          564 c          522 g          533 t
ORIGIN      
        1 attcttcatt cttcattctt cattcttcat tcttcattct tcattcttca ttctatttat
       61 tggctggctg gcgtgaatct tcaccttgct gatcttcaag gagcgccagg atttcttcga
      121 gcaaaatcgt gtgacgttct tcaacttgtt ttaattcagc tacataagcg gcgtggagtg
      181 cgtccacttt ttcgtggagt cgcgaaattt caaattcgga tttcaggttg acttgataat
      241 cctgctcggc ctgcaaccga tctttggccg cctgtcgatt ctggctcatc ataatcacgg
      301 gtgcctgcaa agccgccaga cacgaaagtg ccagattcaa caaaatgtag ggatattgat
      361 caaatgcctt gtccttcaaa accaggctgt tcaatgccat ccagaacaaa agcgtgaaaa
      421 agaacagaaa aatgaagggc cagcttccac caaaggaggc aatctgatca gccactcgtt
      481 gcccaaaggt caactgttcg tcaaattgct ggttgatgtt ggcactgatc aatctccgct
      541 cagccaggcg gcggataaat cgcttttcga tttcagtgag tttccttcgg tcttccgcaa
      601 gcagccgtga tgcaacttgc tcgatttgag gcgtcacagg cagtgtctcc ctctgcaaaa
      661 gatttggaat gatggattgg gggcaaaaaa ccagggcgta gggcaaccga agggatgagg
      721 gatgagggat gagggatgaa agaaaaccag gtcttcatct cgatttcttc atcccgacgt
      781 cttccgcccg ctgtcttcca ctcgcttcga tttttgcttg cgaacaatag tccgcaaaag
      841 caaggttttc aaactgactt catcatcgaa tcgggcttta atttcaagca ctgacacggg
      901 cagtgaccca aaatgaaatt gctcaagctt gacggcatct ttttcagtcg tcaccagtcg
      961 ttcggcgccg gccagttggg cagccgatac aatctcggaa acgtcttttt gggagtaccc
     1021 atggtggtcg cgcaagtgtt tccgaaacac gattttggca ccgagcgtcg ctacatcgtg
     1081 ttcaaaccgg tcagggtttc caagcgccga aactacccca accgctttcc cagccagaga
     1141 gcgttgcgcc tgaggtttac cgtcctgtaa ttggtaaaca ccggtaactt catggaacga
     1201 aaagatgagt ggcacatcca caccgcattc attcaaaatc cagcgcaact catcctcatc
     1261 aaaagcctga tcggcgcggg tcacaatgat tgcgtcagcc cgcttgagcg catagagtgg
     1321 ctcgcgcagg cgtccaaatg gcaccatctc accgccacca aaagggtcag tcgcgtccag
     1381 caccagcagg tttaaatccc gttgaagctg gacatgttga aacccatcat ccagcaaatg
     1441 aacctcacag gcctggttgg cttcaagcca ggtgccgttt gcatggcggt ttttgccaac
     1501 taccactttg accccgctca atcgtcgtgc cagcatcagc ggttcatcgc cagcctcagc
     1561 gagcgtggca caaatctggg tgccgtcact gacgacaacc cgtggctgac gacgatcctg
     1621 tcgagcgtat ccacgggtca aaatggcgac ttcatggcct tcaccagtca ggtaacgggc
     1681 gataaattca accagcggtg ttttcccggt tccaccaagg gtaatatttc caacacttat
     1741 cactggatta ctcagggctt ttggcttcaa ataaccagtt tcatagagtg ccagtcggag
     1801 ccgtacccca aattcataga gctttcccgg cacataaagc caggaccttg ggatgttccc
     1861 ccaaccaggc acctggattc gggttgcgtc agcttcagtt tctggcatag caatctccgt
     1921 gggattagat aaaaacctgg gactgaaaat accagggctt ggggctgagg gcttggggct
     1981 gagggctgag ggctgaaaat atcagggctt ggggctgaag aaaacgggtt caatcccctg
     2041 agccctcagc ccttagggat tgtaaaaaat caacttcccg ttttcagtga ggagcgcagt
     2101 catcctgttc catccggatt tttggttttg caccgcga
//
LOCUS       DAOQDO010001122         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49363_length_2137_cov_2.950528, whole genome shotgun sequence.
ACCESSION   DAOQDO010001122 DAOQDO010000000
VERSION     DAOQDO010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49363_length_2137_cov_2.950528"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    299..322
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<323..1308)
                     /locus_tag="PKZ53_16510"
     CDS             complement(<323..1308)
                     /locus_tag="PKZ53_16510"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ42092.1"
                     /translation="MPEVIQFFDNSGRTLVHRYPPQGSTNITWGSQLIVQENQTAVFF
                     RDGRALDTFGPGRHTLSTMNLPLISSLPGLFFGGNSPFQASVLFIGRQTFQDLKWGTK
                     APILLQDPQLGPINLRAFGKYSLRVVDPQLFSATVVGTRGRVTTEQVEDFLRDLIVQQ
                     LSDLLGENFNSVFKLASLYNEIAAGTKGRAADDFAKQGMELVDLTVGAITLPEEVQKR
                     IDEGAGMRALGDMNAYMQFQTAQALRDAANNPGGAGEGLGLGMGIGMGQAMAGRMAPP
                     PPQYPGYPPPGYPPPPGFPQQPPPGAAAPPVDDIEARLEKLLNLKNKGLITE"
     gene            complement(1463..1969)
                     /locus_tag="PKZ53_16515"
     CDS             complement(1463..1969)
                     /locus_tag="PKZ53_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42093.1"
                     /translation="MFNVNSRKNWLERLADKIPGLGDYLDRDRRREIDKVLREQVADR
                     LRHIKTIPNTVLRDLSDTGRLFEVGPLDRILKKLDKMENRIRFATYGYSGFFDAVKID
                     DFELDQIYQFDLTLLDLAEIIEADANVVAAQAGDATTLKAAAAALEKNIDALDAKFTE
                     RYNKINTI"
BASE COUNT          542 a          533 c          538 g          500 t
ORIGIN      
        1 ggagggtatc atcgggaggc atcagcgaag agcttttcac aatccggtgg ccatttttgg
       61 caaagtaatc aagaaatttt tggcgaattt cgtgtcctgt catttgagtt cctgagaaaa
      121 gggttaaggt gaaatagttc ggtacagtga ctgcccgtgg ataaaaggac tgaaaggacc
      181 gaaaggacaa aaaagaaggg atgaatccag tcccgtcttt tttgtccttt ccgcaccaac
      241 tgcaccggag cgtgttcagc aaggttatca aatttcttcc aagatcttat tacggcggnn
      301 nnnnnnnnnn nnnnnnnnnn nntcttcagt gatcaagccc ttgttcttta agttgagcag
      361 tttttccagt cgagcttcaa tgtcatctac cggcggtgcc gcggcgcctg gtggtggttg
      421 ctgtggaaag cccggcggtg gtggataccc tggcggcgga tagcctgggt actgcggtgg
      481 cggcggcgcc atacgaccgg ccatggcctg tcccatcccg atgcccatgc caagtccaag
      541 tccttcgcca gcgccgccag ggttgttggc ggcatcgcgc agggcctggg cggtttggaa
      601 ttgcatatag gcgttcatat cacccagcgc gcgcatcccg gcgccttcgt caattcgttt
      661 ttggacttct tcgggcaagg taattgcccc aaccgtcaaa tcaaccagtt ccatgccctg
      721 cttggcaaag tcatcggcgg cgcggccttt ggtgccggca gcgatttcgt tgtagagcga
      781 ggccagttta aagaccgaat tgaaattttc acccagcaaa tcagacaatt gctggacgat
      841 cagatcgcgc agaaagtctt caacctgttc ggtcgtcacc cggccacggg tgccaaccac
      901 cgtggctgaa aacagttgtg ggtcaaccac gcggagcgaa tatttcccaa acgcccgcag
      961 gttaatcggt ccaagctgtg ggtcctggag caaaattggt gctttcgtcc cccacttcaa
     1021 atcctgaaac gtctgacgac caataaacaa gaccgaagcc tgaaacgggg aattgccacc
     1081 aaaaaagagc cccggaagtg acgagatcag cggcagattc atcgttgaca gtgtgtgtct
     1141 tcctggtcca aaggtatcga gtgcccgacc atcccggaaa aacacggctg tctgattttc
     1201 ctgaacaatt aattgtgatc cccaggtgat gttggtcgaa ccttgaggtg gataccgatg
     1261 caccagcgtg cggcctgaat tgtcgaaaaa ctgaataact tctggcatgt gaatcacctt
     1321 gttgaatgaa gaattgagaa tgaagaatga agaatgaaga atgaagaatg aatcaaccaa
     1381 tgtgccattt cttctattct cttattccag gttcttctgc cttctgggtt gagaccagtt
     1441 tgaactcggg actaaaaact ggctagatcg tgttgatctt attgtagcgt tcggtgaatt
     1501 tggcatcgag cgcgtcaata tttttctcaa gcgcggcggc ggcggctttc aacgtggtgg
     1561 catcacccgc ctgggcggcc accacattgg catcagcctc aataatctcc gccagatcca
     1621 gcaaagtcag gtcaaactgg tagatttgat caagctcgaa atcgtcaatt ttcaccgcat
     1681 caaagaatcc agagtagcca taggtcgcaa accggatccg attttccatt ttgtcgagct
     1741 tttttaagat tcgatccagc gggccaactt caaacagccg gccagtatcg ctcaaatccc
     1801 gcaaaacagt gttgggaatg gttttgatat ggcgcaaccg gtcagccacc tgttcgcgca
     1861 ggactttatc aatttcacgc cggcggtcac gatccagata atctcccaga ccagggattt
     1921 tatcggccaa ccgttcaagc cagttttttc ggctgttgac gttaaacatc agcaggtctc
     1981 ctgtttgaaa tagaatgaaa aatgtacctg gtacctggtg cctggtgttt aggaagcaat
     2041 actttcaaag aaaaaccagg aaccaagaac caagcaccaa gaaccaagca ccaagaacca
     2101 agaaccaagc accaagaacc aagcaccaag aaccaag
//
LOCUS       DAOQDO010001123         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49366_length_2137_cov_2.697406, whole genome shotgun sequence.
ACCESSION   DAOQDO010001123 DAOQDO010000000
VERSION     DAOQDO010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49366_length_2137_cov_2.697406"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2137)
                     /locus_tag="PKZ53_16520"
     CDS             complement(<1..>2137)
                     /locus_tag="PKZ53_16520"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ42094.1"
                     /translation="VGQVIDPDQKPLAEVVVRLTNLINGFVYAQRTNQEGMYRIELLP
                     AGRYLITAEKEGFQSAAIAEFVVEVNREKLIKPPPIQLLPLAGQQSVPQTQGLTQVNG
                     SDPTIRWSSTSDLIQGLPLAGLRTFDQLALLAPGVAPPPAALGTTGPGIGSGTGTAGQ
                     FSINGQRSRSNTFTIDGSDNNDQEIGVRRQGYLTPVPVLVESITEIQVTTLLANTEAG
                     RNAGGQINVVSIGGGNAVHGEAHAFLTNAALNARDAFDLSSPGNPPKNPFTRTQAGGS
                     VGFPLRPNQLQAFFAFERLELHQTQEHHFVVPTGLERQRSLSRARNRSQSGIDVLSPT
                     FYPLPNTPGGPYGSNTLTQLLPASGAGTLFDARLDGQTHLIGRSSTASLRYALTDDTQ
                     TLPAIDQAINASVQARTQTHNLALIFNTIASPRFANQFRFSFGRTRLAFDELPDSPFV
                     FQSASTGQDLTGDGVPDGRSGPIGRLILAPFSSLGVDPYTFPQARATNTFQVADTAIW
                     SGGHHTIKWGGDLRRIQLNGQLERNYRTQVAFTSGLIGLRDGTLLGGSGVDFAAFGLP
                     SDIAQALALTPDSTLGLRFLELNLFYTHQVRLTSNLALEYGLRYERNTVPTDVSGRLE
                     RSLQATPADLPPFDPQAPFVQSFLKAFQAQQPLLAGRKKIFDGDGNNFAVRVGAAWDL
                     SGHGQTSLRGGYGMFYDPLLTTVVSQSRNA"
BASE COUNT          471 a          563 c          644 g          459 t
ORIGIN      
        1 gcattgcgcg actggctgac caccgtggtg aggagcgggt cgtaaaacat accgtacccg
       61 ccccggagcg atgtttgtcc gtggcctgaa agatcccagg ccgcaccgac tcgcactgcg
      121 aagttgttgc catccccatc aaaaatcttt ttccgacctg ccaaaagcgg ctgctgggcc
      181 tgaaaagctt tcaaaaaact ctgaacaaac ggcgcctgcg ggtcaaacgg aggcaaatca
      241 gccggtgttg cctgcaacga gcgttcaagt cgtccactga cgtcggtcgg tacggtgttg
      301 cgttcatacc ggagcccata ttcgagcgca aggttggatg tcagccggac ttgatgggta
      361 taaaacaggt tgagttcaag aaatcgcaga ccaagcgtcg aatcaggtgt caatgccagt
      421 gcctgagcaa tatcggaagg cagtccgaag gctgcaaaat caactccgct tccgccaagg
      481 agggtgccat cccgcaatcc aatcaacccg gaggtaaaag ctacctgggt gcgataattg
      541 cgctccaact ggccattgag ctggatgcgc cgcagatcac cgccccattt gatggtgtgg
      601 tgacctcctg accagatagc agtatcagcg acttgaaatg tgttggtggc tctggcctgg
      661 ggaaaggtat acgggtcaac gccgagtgaa ctgaagggcg ccagaatcag gcgtccgatt
      721 ggaccgctgc gcccatctgg aactccgtct ccggtcaaat cctggccagt tgaagctgat
      781 tgaaagacaa atgggctgtc cgggagttca tcaaaagcca ggcgggtccg tccaaatgaa
      841 aaccgaaact ggttggcaaa gcggggggaa gcgatggtgt tgaagatcag ggccagattg
      901 tgggtttgag tccgggcctg aacgctggca ttgattgcct gatctatggc cgggagtgtc
      961 tgggtatcat cggtgagcgc ataccggagg cttgccgttg aggaacgccc gatcaggtgt
     1021 gtctggccat caagtctggc gtcaaaaagt gtccccgcac ccgacgccgg gagcaattgc
     1081 gttaaggtgt tgctgccata tggtccacct ggagtatttg gcaatggata gaaggtcggt
     1141 gagaggacat caatccctga ttggcttcgg ttcctggccc gtgacaggct acgctggcgt
     1201 tcaagcccgg tgggcaccac aaaatggtgt tcctgggtct ggtgcaattc cagacgttca
     1261 aatgcaaaaa aagcctggag ttgattgggg cgaaggggga acccaaccga tccgccagcc
     1321 tgagttcggg tgaaaggatt tttgggcgga tttccagggc tggacaggtc aaaggcatca
     1381 cgggcattga gcgccgcgtt ggtcaaaaag gcatgggctt cgccgtgaac ggcattcccg
     1441 ccgccgattg aaacaacatt gatttgccca ccggcatttc ggccagcttc ggtattggcc
     1501 agcaaggtgg tgacctggat ttcggtgata ctttccacca gcactggaac cggcgttaaa
     1561 tacccttgcc gtcgaacgcc gatttcctgg tcgttgttgt cagagccatc aatggtaaag
     1621 gtattgctcc ggctacgctg gccattgatt gaaaactgac ctgcggtgcc agttcctgag
     1681 ccaattccag ggccggtggt ccccagggct gctggcggcg gggccacgcc gggtgccagc
     1741 aaagccagtt gatcaaacgt gcgcagtcca gccagcggga gtccctggat caagtccgat
     1801 gtgctgctcc agcgtatggt cggatcgctt ccattcacct gggtcaggcc ctgcgtctgt
     1861 ggcaccgatt gttgaccggc aagcggaagg agctgaattg gaggcggttt gatcaatttt
     1921 tcccggttga cctcgaccac aaactccgca atcgcggctg attgaaatcc ttctttttcc
     1981 gccgtgatca gataccgccc ggcagggagc aattcgatgc ggtacattcc ttcctgattg
     2041 gtacgctggg catagacaaa cccgttgatc aggtttgtga ggcgaacgac cacttcggcc
     2101 aggggttttt ggtctgggtc aatcacctga cccacca
//
LOCUS       DAOQDO010001124         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49403_length_2136_cov_3.242672, whole genome shotgun sequence.
ACCESSION   DAOQDO010001124 DAOQDO010000000
VERSION     DAOQDO010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49403_length_2136_cov_3.242672"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="PKZ53_16525"
     CDS             complement(<1..1646)
                     /locus_tag="PKZ53_16525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42095.1"
                     /translation="MSTLGKICPTCKQKFTATTNFCPTDSEVLEFDLNSLVGTLLDGQ
                     YQVEEFLGKGGMGAVYRARHSRLGDMVAIKILPLHLSANPEYQRRFVREGQAARLFRH
                     PNAVAVFDLRETSDGLLYMVQEFVKGQTLTKELKKRRILSPTEAFYLLAPVMGALQAA
                     HQSGVVHRDLKPDNIMIGTDAEGNQLVKLLDLGIAKIRETSDITALTMTGQLLGTPFY
                     MSPEQWDGSEDIDGRADIYSLAIILYELISGKRPFEGKTLESLAYLHTFTKAKPLHEL
                     RSSIPYNFSRIIERAMEKERNLRPPTMLALMEELRQALLELKIGIGGPVTDEQEAAVT
                     LFVPTDVHPTNPGLDTHQKGATRTLIDVPENSQSDQLRATAGTPEALVTIIDSASSPI
                     QKSIPPRDLSVTRVPVKPELPKKEISESKSLVDPGLTAPTLKLGPEHAALHPVPSPSG
                     SENLTAEPSNLSTSFVAQPDMGTGRFSINQTLVASLAGFLVMIVAGVIFWPRFTPPPP
                     LQPPPVTKTLAMTEVLQFWLTVAGKAGTITTQQFADTPEL"
     gene            1827..>2136
                     /locus_tag="PKZ53_16530"
     CDS             1827..>2136
                     /locus_tag="PKZ53_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42096.1"
                     /translation="MDNLRFIREAMENASAFTAVPGWGGVWMGITALLAATWAANLSS
                     PTRWLQAWLVEAGVAIAIGFGMMIRKARIVNISLLSVPSRKFVLSLSPPLLVGGLLTV
                     V"
BASE COUNT          480 a          534 c          582 g          540 t
ORIGIN      
        1 tggagttctg gtgtgtcagc aaattgctgg gtggttatcg tgccagcttt tccagcgacg
       61 gtgagccaga attgaagcac ttcggtcatg gccagcgtct ttgttaccgg tggaggttga
      121 agtggaggtg gaggggtgaa gcgaggccaa aaaatgacac ctgccacgat catcaccaaa
      181 aaaccggcca ggctggcaac cagggtctgg tttatcgaaa accggcctgt ccccatatca
      241 ggctgggcca caaacgaggt actgaggttg gatggctcag cagtgagatt ctctgaacca
      301 gatggcgatg gaactggatg caaagcagcg tgttcaggcc ctaacttgag tgtcggtgcc
      361 gtcaaaccag ggtcaacaag cgattttgac tcggaaatct ctttttttgg aagctctggt
      421 ttcacaggga cgcgagtcac tgacagatca cgtggcggga tacttttttg aatcggtgat
      481 gaggctgaat ctatgatggt caccagtgct tcgggtgtac cagccgttgc ccggagttga
      541 tcgctctgag aattttcagg gacatcaatc agtgtcctgg tggccccctt ctggtgtgtg
      601 tctaatccag ggttggttgg gtgtacatca gtgggaacga aaagggttac ggcggcttct
      661 tgttcatcgg ttaccgggcc tccgattcca atctttagct ccaggagggc ctggcggagt
      721 tcttccatca aggccagcat tgttggaggg cggaggtttc gttctttttc catcgcccgt
      781 tcaatgatcc gactgaagtt atatggaatc gaacttcgta actcgtgcag cggtttggcc
      841 tttgtgaacg tgtggagata ggccaggctt tccagtgttt ttccttcaaa agggcgtttg
      901 ccactgatga gttcatacaa aatgatcgcc agactataga tatctgcccg tccatcaata
      961 tcttcagatc cgtcccactg ttctggcgac atataaaacg gagtgcccag taactggcca
     1021 gtcatcgtca gggcagtgat atctgaggtt tcacggatct tggcaatccc cagatccagc
     1081 aacttgacca gttgatttcc ctcggcatca gtcccaatca tgatgttgtc tggctttaaa
     1141 tctcgatgga caacgccgct ctggtgtgcc gcctgcaggg cccccatcac cggagccagc
     1201 aggtagaagg cctcggttgg ggaaagtatc cggcgttttt ttaattcttt ggtcagcgtc
     1261 tgccctttga caaactcctg aaccatatag aggaggccat cgctggtttc gcgcaaatca
     1321 aagaccgcca ctgcattggg atggcgaaac aagcgtgcgg cctggccttc acgcacaaat
     1381 cgtcgctggt attcggggtt ggcactcaaa tgtaagggga gaatcttgat ggccaccata
     1441 tccccaaggc ggctatggcg agcgcgatac acggccccca tgccgccttt tccaaggaat
     1501 tcttcaacct gatactgtcc atccagcagt gtcccgacca gagaattcag atcaaattcc
     1561 aggacttcgc tatccgttgg gcagaaattg gttgtggccg taaatttctg cttgcaggtg
     1621 ggacagattt ttccaagggt ggacataggt aaatcgctcc aactcaggat gagtctcaat
     1681 aaatgaaagc tggagatttc gcaatagaac tttatttcaa aaagtacttg tcaacaataa
     1741 gatccttatg taactatccg cccgtggctg cgattcatcc tttcccaaaa caaccaaacg
     1801 accctccagc actccacgcg cgtgcaatgg ataatctgcg ctttatccgc gaagcgatgg
     1861 aaaatgcttc cgcgtttacc gcagtgcccg gctggggagg tgtctggatg ggcattacgg
     1921 cccttctggc ggcaacctgg gcggccaacc tgtcatcacc aacccgatgg ctccaggcct
     1981 ggctggtcga agccggcgtg gcgattgcca ttgggtttgg catgatgatc cgaaaagcac
     2041 ggatcgtaaa catttcttta ttgtccgtcc ccagccggaa atttgtgctg agtttatcgc
     2101 cacctctgct ggtcggcggc ttattaacgg ttgtga
//
LOCUS       DAOQDO010001125         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49456_length_2135_cov_3.299519, whole genome shotgun sequence.
ACCESSION   DAOQDO010001125 DAOQDO010000000
VERSION     DAOQDO010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49456_length_2135_cov_3.299519"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            582..1991
                     /gene="glnA"
                     /locus_tag="PKZ53_16535"
     CDS             582..1991
                     /gene="glnA"
                     /locus_tag="PKZ53_16535"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNJ42097.1"
                     /translation="MDPKTVLQYAVDKQIKFVDVRFTDLPGSWQHLTFPISELHEDTF
                     ENGFGFDASSLRGWAAINESDMLLIPDPTRFWVDPFFEEDTLCLFADVVDPITKEGYP
                     LDPRSVAKRAESYLKFTGLADTAYFGPEAEFFVFDSVRYKNDPNCAMYEFDSVEGNWN
                     SGRKQDNLGFRIRTKEGYVPVPPMDSLHDLRSEIMINLESIGIHPECHHHEVATGGQC
                     EIDFRFSTLLGTADNVLLFKYIVRNTAYQYGKAATFMPKPLFGDNGSGMHCHQSLWKD
                     GKPLFAGDNYAGLSEMALHYIGGLLKHAPAIIAFAAPTTNSYKRLVPGFEAPVNLAYS
                     ARNRSAAIRIPMFSSNPKQKRLEFRPPDPSCNPYLTFAALLMAGLDGIQNKIDPGDPL
                     DKDIYDLEPEELKKVASLPGSLEESLNALEKDYEFLLKGDVFTEQLIERHISYKRKRE
                     VDVMRMRPHPLEFQMYFDI"
BASE COUNT          511 a          537 c          536 g          551 t
ORIGIN      
        1 gggtggaaga tatgccggcg gaagcctacg tccagtctgg attccggctg gcgacgcaag
       61 gctatgcggg ggaagacgta ccaaacacct ctggttcgac tgagggaaat acaattcttt
      121 caataattta gccttctctt cattcgaaga cttcattccg aaatggtatg aatttgtgtt
      181 gtctggggtg tggctccacc gcaaatttga tgcccgttaa tcgttgccca atatgggacc
      241 ttttcaagtc gtcttgacag catcatcccc agtgaacatc atgtgacggc cctttacatt
      301 taggttactc cctctgccac tggctcattt tctgtaaatc cttgtattct ggcaggttgt
      361 gactgcttct tgcagaccga gatgtaaagc aacaaacggc ctatccaagt tttcgcggct
      421 gtccccctcg gcacttcgat ccaaaatgca cactgacact ctagcaattg agcctttgct
      481 ttgtcttttg acagaccagg gccgtgtcgt cgtgtgtgtt ttttcagttc ccgaggttgg
      541 cgcctgcgac catctttatt gagattgtga agagaggaaa aatggaccca aaaactgtcc
      601 ttcaatatgc tgttgacaaa caaatcaagt ttgttgatgt ccgctttacc gatctgccag
      661 gaagctggca gcacctgacg tttcccatca gtgaattgca cgaagacaca tttgaaaatg
      721 gctttggctt cgacgcatcg agcttgcgcg gatgggcggc gatcaatgaa agcgacatgc
      781 tcttgattcc ggacccgacc cgattctggg tggatccgtt cttcgaagaa gacaccctgt
      841 gcctgtttgc ggatgtggtt gatccgatta ccaaagaagg ttacccgctt gatccccgct
      901 cagttgccaa acgggcggaa agctatttga aatttactgg tctggcggat acggcgtatt
      961 ttggcccgga agctgaattt tttgtttttg acagcgtccg gtacaaaaat gatcccaatt
     1021 gcgccatgta tgagtttgat tcagtcgaag gcaactggaa cagcggtcgc aagcaagaca
     1081 accttggttt tcggattcgg accaaggaag ggtatgttcc agtgccgccg atggattcac
     1141 tccatgacct gcgctcggaa attatgatca acctggaatc aattggtatt cacccggaat
     1201 gccaccatca cgaagtcgcg accggcggcc agtgcgaaat tgatttccgg ttttcaaccc
     1261 tgctgggcac ggctgataac gtgttgctct ttaagtacat tgtccgcaac acggcctatc
     1321 agtatgggaa agcggcaacc tttatgccca aaccgctgtt tggcgacaac ggctcgggga
     1381 tgcactgcca ccagtcactc tggaaagacg gaaaaccact ctttgccggg gacaactacg
     1441 ccggattgtc ggaaatggcg ctccattaca tcggcggtct gctcaaacac gctccggcga
     1501 tcattgcctt tgcggcgcca acgaccaata gctacaagcg attggtgcct gggtttgaag
     1561 ccccagtcaa cctggcgtac tcagcccgca accgttcagc cgcgattcgg attccgatgt
     1621 tttcatccaa tccaaaacaa aagcggctgg agttccgacc gccggatcca agctgcaacc
     1681 catacctgac ctttgctgcc ttgttgatgg ccggtctgga tggaattcag aacaaaattg
     1741 atcccggaga tccgcttgat aaggatattt atgatctgga gccggaagag ttgaagaaag
     1801 tggccagctt gccggggtcg cttgaagaat cactcaatgc gcttgaaaaa gattatgagt
     1861 tcctgctcaa gggcgatgtc tttaccgaac aactcatcga acgtcacatt tcctacaagc
     1921 gcaagcgcga agtggatgtg atgcgcatgc ggccacaccc actcgaattt cagatgtact
     1981 ttgatatcta atgccagttt gtggtcagtg gtcagtggtc agtgatcagt cgttcactca
     2041 gtttatttgg ttgaatcact tgactgttct ctcctgtcgc caggagtaaa tcaccacttg
     2101 ccaggccaaa aagaaaaggt gatggaaatt acttc
//
LOCUS       DAOQDO010001126         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49498_length_2134_cov_3.339105, whole genome shotgun sequence.
ACCESSION   DAOQDO010001126 DAOQDO010000000
VERSION     DAOQDO010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49498_length_2134_cov_3.339105"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKZ53_16540"
     CDS             <1..298
                     /locus_tag="PKZ53_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42098.1"
                     /translation="VIGNIFHRTFIKGLALYTPIPWPHGVPTMPEMDQEKQGTPPVEF
                     TRDMAELVAIIERIHRPNRDFAWGRHPIFNEMSDWEWLRWGYLHVDHHLRQFGV"
     gene            complement(436..>2134)
                     /locus_tag="PKZ53_16545"
     CDS             complement(436..>2134)
                     /locus_tag="PKZ53_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ42099.1"
                     /translation="CAVTPPQNVRNTMIVTVDWLAKHLNDSSLVLLHVGEKTEFEKEH
                     IPGAIYISVSDISLSQAETPLTLQLPPAERLKETFEKMGVGDNSRIIVYFGKDWVTPT
                     TRVYFTLDYLGLGDQTSILDGGLPAWKAAGQSVTAEVKSPVRATLTPHPNPDLVASLD
                     WVKSHLNKPDTALIDARVSRFYEGRDPGAMPRAGRIPGATNIPYPSLTYEADLKFKDP
                     ATLATMLRQAGVDQGDTVVSYCHIGQQATSVYFVARYLGFKARLYDGSFDEWSRRDDL
                     PVDGLNPSELAQTRLTVVTPEWVTQHANDPNLRIVDTRLNPYDYFAGHLPQAVHLADA
                     SLRGPSAGLPVQFYQPGLLGFMLSRAGIKAGDKVVVYSDGMDVLGATMTAYVLERLGH
                     KEIYFVDGGWTAYKATQKPSQEYPKYQAVDYDLTDARKAVSVTLDELKALMGKSGVVL
                     IDARPAPTYRGDTNVWTRNGHIPGAINLEWRSLTDPDNPHKLKPVEELRAIVGARKLD
                     PASDIIVYCGTGREATLEYMVLKHVLGFRKVRLYEGSWTEYCAQPELQIETGPEKKN"
BASE COUNT          481 a          551 c          579 g          523 t
ORIGIN      
        1 ggtgattgga aatatctttc accgcacgtt catcaagggc ctggcgctgt acacgccgat
       61 tccctggccg catggtgttc cgacgatgcc ggaaatggac caggagaagc agggaacacc
      121 acccgttgaa tttactcgtg atatggccga actcgtggcc atcattgaac gcatccaccg
      181 acccaatcgc gattttgcct ggggccgtca cccgatattt aatgaaatga gcgattggga
      241 gtggctgcgg tgggggtatc tccacgtaga ccatcacctg cgccagtttg gcgtgtaacc
      301 ctgaacaaag atgaaggctt tttcccaggc ttcagacgaa gaaaccacgg aacacacaga
      361 atacacagaa aataagtcaa gattcttcaa tggtttctga gattccaatg gatggctgac
      421 tcctgatcac tgacatcaat tcttcttttc cgggccggtt tcgatctgga gttccggttg
      481 agcacaatat tctgtccagg atccttcata gagtcgcact ttgcggaacc caagcacgtg
      541 tttaagcacc atgtattcca gggttgcttc gcgaccggtt ccgcaataga caatgatgtc
      601 actcgccgga tcaagcttgc gtgctccgac aattgcccgg agttcttcga ctggtttcaa
      661 tttgtgcgga ttgtccggat cggtcaaaga tcgccactca aggttgatcg ctcctggtat
      721 gtgtccgttg cgtgtccaga cattggtgtc accccgatac gttggtgccg ggcgagcgtc
      781 aatgagcacc acacctgatt tgcccatcag tgctttgagt tcgtcgagtg tcactgacac
      841 tgcctttcgg gcgtcagtca ggtcatagtc aaccgcctga tattttggat attcctgcga
      901 aggtttttga gtggctttgt aggccgtcca gcctccgtca acaaagtata tttctttgtg
      961 tccaagccgt tcgaggacat aggcggtcat cgttgcaccc agtacatcca taccatctga
     1021 atacacaacc accttatctc cggctttgat tccagcccgg ctcaacataa agccaagcaa
     1081 acccggctgg taaaactgaa cgggcagtcc agccgacggg ccgcgcaacg aggcgtcagc
     1141 cagatgaacg gcctgtggga ggtgtccggc aaagtagtca taggggttga gccgggtatc
     1201 aacaatgcgg aggtttggat cattggcgtg ctgggtcacc cactctggtg tgaccaccgt
     1261 gaggcgggtt tgagcgagtt cagatgggtt cagcccgtca accggcaggt catcacggcg
     1321 gctccattcg tcaaaagatc catcatacaa ccgtgctttg aagcccagat acctggcgac
     1381 aaaatagacg gatgtcgcct gttgtccgat gtggcaatac gaaacaaccg tatcgccctg
     1441 gtcaaccccg gcttgcctga gcatcgtcgc aagtgttgcc ggatccttga atttcaagtc
     1501 ggcttcatag gtcagtgatg gatacggaat attagtcgcg cctggaatgc gtccggcacg
     1561 aggcatcgcg ccaggatcac ggccttcata aaatcttgag acccgggcat caatgagggc
     1621 agtatctggt ttgtttaaat gacttttcac ccaatcaagc gatgccacca ggtctggatt
     1681 cgggtgcggg gtgagggttg cgcggacggg tgatttcacc tcggctgtca ctgactgtcc
     1741 agccgctttc caggcaggta gtccaccatc gaggattgac gtctggtcgc caagtccaag
     1801 gtagtcaagc gtaaaataga cccgtgttgt cggtgtgacc caatctttac caaagtagac
     1861 gataatccgt gaattgtcgc caacgcccat tttttcaaag gtttctttca ggcgctccgc
     1921 tggtggaagt tgcagcgtga gtggggtttc agcctggctg agggaaatgt cggatacgga
     1981 aatgtatatc gcgccaggaa tgtgctcttt ttcaaattct gttttctccc cgacgtggag
     2041 tagaaccaga gacgagtcat ttagatgttt ggccagccag tccacggtga cgatcatcgt
     2101 atttcgcaca ttctggggag gcgtgacggc acat
//
LOCUS       DAOQDO010001127         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49536_length_2133_cov_3.481232, whole genome shotgun sequence.
ACCESSION   DAOQDO010001127 DAOQDO010000000
VERSION     DAOQDO010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49536_length_2133_cov_3.481232"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2133
                     /locus_tag="PKZ53_16550"
     CDS             <1..>2133
                     /locus_tag="PKZ53_16550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42100.1"
                     /translation="LAALYDTKDDFVKTEPLLVRALAIYEKVLGPDHSYVATSLNNLA
                     RFYMNRGNYEKARSLFRRSLAIYEKTLGPEHEYTAACLTNLASLAHTNSNFSEAVALL
                     KQALNIFEKAIGTDHPTTAICRNNLAEAYRELGDYTRIEPLYRQCLASFEKSVGPDHP
                     YVGFIYNNLGNLYHDRGDYTQAETCYLRSLAIREKALGPDHLDVAQSLNNLASLMMEK
                     GDLVQAEPLLQRSLAIRKNVLGPDHPDVALMLTNLGALYERRNDYAQAEQYNQQALAI
                     YSQKFGQNHRSIAASLKNLAVIYTNQNRFSEAESLLQRSQAIFETVFGPVHPEVAENL
                     IQMANVYATKGDYNQALPPAHRALAIREKTFGTGHPNVAWSLYKLALLHLDNGEPVQS
                     LPFLIRGNDTTEQDLNLNLVSGSERQKALYLKRTEQMSDITLALHLQYLFQNPTAAQA
                     AFEVLLRRKGRPLDVMTNAIAVLRSRSDSKTQALLDDYASVASQVAVQMLRGPGKKAP
                     ADHQAELQALERQKETLEAEISRRSNEFKVQTAPITFEAVKQRIPPNAALIEFTVYHS
                     YDTKLRKPGKPHYAAYVLKGSPDAKGSNPPQLQWVDLGETEPIDQAISQWRQVLRLPS
                     SQLNQPRNLTVVPDQQPVLPSVKEQARLLDRLLMQPVRQLAASCKRFLISPDGALNLI
                     PFDALVDEHGHYLVENYEISFLTSGRDL"
BASE COUNT          560 a          561 c          516 g          496 t
ORIGIN      
        1 cctggccgcg ctctatgaca cgaaagacga ttttgtcaaa actgaaccgt tgctggtacg
       61 ggccctggcc atttatgaga aggtgcttgg tcctgatcac tcgtatgtgg cgaccagtct
      121 caataacctg gcgagattct atatgaatcg gggtaactat gagaaagcca ggtcactttt
      181 caggcgttca ctggccatct atgaaaaaac attaggtcca gagcacgagt acaccgccgc
      241 ctgcctgacc aatctggcct ctctggctca tacaaacagc aatttctctg aggcagtggc
      301 cttattaaag caagcgctga acatatttga aaaagcgatt gggactgatc acccaacgac
      361 tgcaatttgt cgcaacaacc tggctgaggc atatcgggaa cttggtgact atacccggat
      421 cgagccgttg taccggcaat gcctggccag ttttgagaaa tcggttggtc ctgatcatcc
      481 atatgtgggt ttcatctaca acaatcttgg aaatctctac catgatcgag gcgattatac
      541 ccaggctgaa acctgctatc ttcggtcgct ggccattcgg gaaaaagccc ttgggcctga
      601 tcatctcgac gtcgcccaaa gcctcaacaa cctggcttcc ctgatgatgg aaaaaggaga
      661 cctggtccag gcagagccgt tgctccaacg gtcccttgcc attcgcaaaa acgtgcttgg
      721 gcccgaccat cccgatgtgg ccttaatgct cacaaacctg ggggccctgt acgagcgcag
      781 aaatgactat gctcaggctg aacaatacaa ccagcaggca ttggcgattt attctcaaaa
      841 gtttggccag aaccaccgca gtattgcggc ctcgctgaag aacctggcgg tgatttatac
      901 caatcagaac cgattttctg aggccgaatc acttttgcag cggtctcagg ccattttcga
      961 aacagtattt ggccctgtcc atccggaggt tgccgaaaat ttgatacaga tggcaaatgt
     1021 atatgccacc aaaggtgatt ataaccaggc gctgcctcca gcccaccggg ccctggccat
     1081 tcgcgagaaa acatttggaa ctggtcaccc gaatgtcgcc tggagcctct acaaactggc
     1141 attgcttcat ttggataacg gtgaaccggt acaatcactt ccatttctga ttcggggaaa
     1201 tgatacaact gaacaggatt taaatctgaa tttggtttca ggatctgagc gacaaaaagc
     1261 gctttattta aaacgaaccg aacaaatgtc agatatcact ctggcactcc acctccaata
     1321 cctgtttcag aatccaactg ccgctcaggc agcattcgag gttctgctca gacgcaaagg
     1381 gcggccactt gatgtcatga ccaatgccat tgccgtttta cgcagtcgca gtgattccaa
     1441 aacccaggcg ttgcttgatg actatgccag tgtggccagt caggtggctg tccaaatgct
     1501 gagagggccg ggcaagaaag cacctgctga ccaccaggcg gaattgcagg cattggaacg
     1561 ccaaaaagag acgctggaag ctgaaatcag ccgtcggagc aatgaattta aagtacagac
     1621 ggcaccgatt acatttgagg ccgtaaaaca gcgaattccc ccaaatgctg cactcattga
     1681 atttaccgta taccattcat atgacaccaa actccggaag ccagggaaac cacattatgc
     1741 agcgtatgtt ctcaaaggct cgcccgatgc caaagggagc aaccctcctc aacttcagtg
     1801 ggtagacctg ggtgagaccg aaccgataga tcaggccatt tcacagtggc ggcaggtgct
     1861 gcgacttccc agttctcaac tcaaccaacc tcgcaactta accgtggtgc cagatcaaca
     1921 accggtactc ccatccgtaa aggaacaggc ccggcttctg gaccggttgc tgatgcagcc
     1981 agttcgtcaa ttggcggcat cttgcaagcg gtttttgatt tcaccagatg gggcactcaa
     2041 tttgattccc tttgacgccc tggtggatga gcacgggcac tatctggttg aaaattatga
     2101 aatttcattt ctgacaagcg gacgtgattt gtt
//
LOCUS       DAOQDO010001128         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49537_length_2133_cov_3.404716, whole genome shotgun sequence.
ACCESSION   DAOQDO010001128 DAOQDO010000000
VERSION     DAOQDO010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49537_length_2133_cov_3.404716"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PKZ53_16555"
     CDS             complement(<1..1231)
                     /locus_tag="PKZ53_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42101.1"
                     /translation="MRRFRHLLLAFPTATIVISLGLISLLTLTGTQFQSVTEVLSVTA
                     WGAGTPSSSTITAFATGRPWWKRHLPEAIQPSPAGGIVPLVRVPRTKIQTTDELILPE
                     RSIGYDAFLGAVTALRSLFSTDLIFDFALLIRLQHWLLVLGICLLPVVFHRIHPAYRT
                     PLTFSTFFIGYLFVLLLRPNKSKFLTDGLIDSALANALALFSVAAFWLILSKSRLENW
                     KQRVVVMSAGLFLSFVTQVRGEFLIVGGLTFGLLALLVWKQPAFSWKGLVLAGLCFVM
                     LPVGYGSFNQQQFGHFVPLRMRSGQNLFEPIGQFPNPWGIQYSDSWLEKYLNQRGIEY
                     TSFEADRVLTAQYVQCLKENPWLFVRNFHLRLWQFAKQFGLWLNFWTIPLILFGFWVA
                     IQKSERLAFLAVPLILAI"
     gene            complement(1243..2055)
                     /locus_tag="PKZ53_16560"
     CDS             complement(1243..2055)
                     /locus_tag="PKZ53_16560"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ42102.1"
                     /translation="MGLVGDLQYLSLADIIQINCIGRNTARLTVHYPIGDGVFYFADG
                     DVVDAKLGKLTGVEAVYKALEYDQGSFRVDSGIRAPTRTIHDNWSNILMEGLRLLDEA
                     RAGLIPGSAPLSAEQINATTTGASRVQNPFQILVSDLTKIKGIDGALAATRDGTLNAC
                     IGVNLSDKVGMMTSLLIYLNQAMATGQGRFGKVKQMDIRYGTKEVMVFDQGTVLVVIE
                     FANTIRFETISPHIDRAFRKFEARSRGGTSALDPSKIGTGSMSGPPDFLPGR"
BASE COUNT          554 a          540 c          596 g          443 t
ORIGIN      
        1 cgattgccag aatcaatgga acggccagaa acgccagtcg ctcagacttt tgaatcgcga
       61 cccagaaccc gaacaagatc agcggaatgg tccaaaagtt gagccataag ccaaactgtt
      121 tcgcaaattg ccagagtcga aggtgaaaat tgcggacaaa gagccaggga ttttccttca
      181 ggcactgcac atactgagcg gtcagaacgc ggtcggcttc aaaactggta tattcgatgc
      241 cacgctggtt caggtatttt tcgagccagg aatcgctgta ctggatgccc caggggttgg
      301 gaaactggcc aattggttca aaaagattct gccccgaacg catccgcaat gggacaaagt
      361 gaccgaactg ctgctggttg aaagagccgt acccgacggg gagcatgaca aaacacaacc
      421 cggccagaac cagtcctttc caggaaaaag caggttgttt ccacaccagg agtgccagca
      481 aaccaaacgt caatccaccc acgatcaaaa attcgccgcg aacctgggtg acaaaactca
      541 gaaacaaccc ggcactcatg actacgacac gctgtttcca attttcaagc cggctcttgc
      601 tgaggatgag ccagaatgct gcaacgctaa agagagccag ggcattggcg agggcactgt
      661 caatgagtcc atcggtcagg aatttacttt tatttgggcg cagcaacagg acaaacaggt
      721 atccgataaa aaatgtgcta aaggtcagcg gggtgcggta cgccggatga atgcgatgga
      781 aaacgacagg cagcaggcag attccgagca ccagaagcca gtgttgcagc cgaatcagca
      841 gggcaaagtc aaagatcaga tcggttgaaa acaggctgcg cagggcggtg actgcgccca
      901 gaaaagcgtc ataaccgata ctgcgttcgg gcaggatcag ttcgtcagtg gtctggatct
      961 tggtccgggg aacacgtacc agcggcacta taccgccggc tggtgagggt tgaatggctt
     1021 cgggaaggtg acgtttccac cacggacggc cagtggcaaa ggcggtgatg gtactgctcg
     1081 acggggtgcc ggcaccccag gccgtgaccg acaaaacttc ggtcacggat tgaaactgag
     1141 tgccagtcag ggtcaggagg ctgatcagtc ccagactgat cacgatggtg gcggtgggaa
     1201 aagccaggag aaggtgtcgg aagcgccgca tgaggacccg ccttaccgcc caggcagaaa
     1261 atcgggtggt ccactcatgg agccggttcc aattttgctt ggatccaggg cactggtgcc
     1321 gccacgtgat cgggcttcaa atttccggaa ggcgcgatca atgtggggtg aaatggtttc
     1381 aaagcgaatg gtattggcaa attcaatcac caccaggacc gtcccctggt caaagaccat
     1441 gacttctttg gtgccgtacc ggatgtccat ttgcttcact ttcccaaatc gcccctgtcc
     1501 ggtggccatt gcctgattca ggtaaatcag aagtgaagtc atcatcccga ctttatctga
     1561 cagattgaca ccaatgcagg cattgagtgt tccatcgcgg gttgcggcca gggcaccgtc
     1621 aatcccttta attttggtca gatccgagac cagaatttga aacgggtttt gaactcgcga
     1681 ggcgccagtg gtggtggcat tgatctgttc ggcagacaag ggagcactgc cgggaatcaa
     1741 tccggctcgg gcctcatcga gcaggcgcaa cccttccatt aaaatattgc tccagttgtc
     1801 atgaatagtt ctcgttggcg cccggattcc gctatctacc cgaaatgagc cctgatcata
     1861 ttccagggct ttatacacgg cttccacacc ggttaatttc ccaagtttgg catcaaccac
     1921 gtccccatct gcaaaataga aaacgccatc cccaattggg taatggacgg tcagtcgggc
     1981 agtgttgcgg ccaatgcagt tgatttgaat gatgtcggca agactgagat attggagatc
     2041 tccgaccaat cccataacac gacctctttt ttagggttca gggtttaggg ttcagggttt
     2101 aaagacattc gggctgaaga aatagggctg ggg
//
LOCUS       DAOQDO010001129         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49591_length_2132_cov_2.974482, whole genome shotgun sequence.
ACCESSION   DAOQDO010001129 DAOQDO010000000
VERSION     DAOQDO010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49591_length_2132_cov_2.974482"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..778
                     /gene="tsf"
                     /locus_tag="PKZ53_16565"
     CDS             116..778
                     /gene="tsf"
                     /locus_tag="PKZ53_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNJ42103.1"
                     /translation="MAEITAAAVKALRDKTGAGMMECKSALVEAAGDEEKAIVILRKR
                     GIMKAESKQSRVAAEGLVESYIHAGGRIGVLVEVNCETDFVARGDEFRELVRDIALQI
                     CAMDAQFVTPDEIPADVIAKEREIALAQVAGDPKTANKPEAIRNNIVESRINKFKAEV
                     CLIDQPFVKDQSITINDLLKGKIQKTGENIRIRRFVRFKLGEGIEKKEVDFASEVAAM
                     QR"
     gene            870..1655
                     /gene="pyrH"
                     /locus_tag="PKZ53_16570"
     CDS             870..1655
                     /gene="pyrH"
                     /locus_tag="PKZ53_16570"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015200357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNJ42104.1"
                     /translation="MSAASENTSSTETSHPTPRFKRILLKLSGEALEGSRGFGIDPTT
                     VRQIAEQVRDVHALGVEIALVVGGGNIVRGAEASSAGIDRAQGDYMGMLGTVINCLAL
                     QSALEKLNIHTRVISAIEMRQVAEPFIPRRAIRHLEKKRVIIFGAGTGSPFFTTDSAA
                     ALRALEIKAEAILKATKVDGIYTADPVSDSSAERYTHVTYQQVLEKGLNVMDASAIAL
                     CKDNQMPIFVFNLLVSGNIFRVVTGESIGTFVGAGATQLAAHG"
     gene            1702..>2132
                     /gene="frr"
                     /locus_tag="PKZ53_16575"
     CDS             1702..>2132
                     /gene="frr"
                     /locus_tag="PKZ53_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNJ42105.1"
                     /translation="MTADIIKDAVARMDATVEDVRRKLTSFRTGRASVSLLDRIVVES
                     YGTEMPLNQVGSINAPEPAMLVVQPWDPSLIGAIERAIRSSDLGLNPSNDGKVVRIPI
                     PPLTQDRRKQLAKAVRDTAEEHRAGLRNIRRDANDHLKKLL"
BASE COUNT          537 a          518 c          578 g          499 t
ORIGIN      
        1 ggctatgaat gatgaaagac gtatctgatt gaataacaga tacttagctg cttcaggatt
       61 cataatccat cactcatact tcgtaacgta ctcgatagag ttttttggag gtcgcatggc
      121 ggaaattacc gcagcagcag tgaaggccct gcgtgataaa acgggcgcag ggatgatgga
      181 atgcaaatcc gctctggttg aagcagcggg tgatgaagaa aaagcaattg ttattttgcg
      241 caagcgcgga attatgaagg ctgaatcaaa acagtcccgt gttgcggctg aaggacttgt
      301 tgagtcctac atccacgccg gtggccgaat tggggtgctg gtcgaagtca attgcgaaac
      361 ggactttgtc gcccgtggtg atgagttccg cgaactggta cgcgacattg ccttgcagat
      421 ttgcgcgatg gatgcccagt ttgtcacccc tgatgaaatc ccggctgacg tgattgccaa
      481 agagcgcgaa atcgccctgg cacaggtggc cggtgatcca aaaacggcaa acaagccaga
      541 agccatccgc aataacattg tggagagccg catcaacaaa ttcaaagccg aagtttgctt
      601 gattgatcag ccatttgtga aagatcaaag catcacgatc aatgatttgt tgaaagggaa
      661 aattcagaag acgggtgaaa acatccggat tcgacgtttt gtgcgcttca aacttggtga
      721 aggcatcgaa aagaaagaag ttgacttcgc cagtgaagtc gccgcaatgc agcgctaagc
      781 acattgctcc aacgccccgc ctaaggctac tcaggcgggg cgtttgtttc tcatctgatt
      841 tttttccggc atcaggagtt aagacaggaa tgagtgccgc cagcgaaaac acatcatcaa
      901 ccgaaacctc ccaccctact ccacgattca aacggattct gctcaaactc agtggcgaag
      961 cccttgaagg aagtcgtggc tttggtattg atcccaccac cgtcagacaa attgccgaac
     1021 aggtccgcga tgttcacgct ctcggggttg aaattgccct ggtcgttggc ggcggaaata
     1081 tcgttcgtgg ggccgaagcc agctcagccg gcattgatcg cgcccagggt gattatatgg
     1141 gcatgctggg cacggtgatt aactgtctgg ccctgcaaag tgcccttgaa aaactcaaca
     1201 tccatacccg ggtgatctct gcgattgaaa tgcggcaggt tgcggagccc tttattccac
     1261 ggcgcgccat tcgccacctg gagaagaagc gggtgattat ctttggcgcc ggaactggaa
     1321 gtcctttttt tacaaccgat agtgctgccg ccttgcgtgc cctggaaatc aaagccgaag
     1381 cgattctcaa agccaccaaa gtcgatggga tttatactgc tgacccggtt tctgatagta
     1441 gcgccgagcg ctatacccac gtgacttatc agcaggtact tgaaaaaggt ctcaatgtca
     1501 tggatgcttc ggcgattgct ttgtgcaaag acaatcaaat gccgattttc gtgtttaact
     1561 tgctggtttc tggaaatatt tttcgggttg tgaccggtga atcaatcggg acttttgttg
     1621 gagctggcgc gacgcagctc gccgcccacg gttagctcat atcagtctga cacccaaacg
     1681 aaagaggaag ggaaaatcaa tatgactgct gacatcatta aagatgccgt tgcacgaatg
     1741 gatgccactg ttgaggatgt gcgccgtaaa ttgaccagtt ttcgtaccgg gcgcgcttca
     1801 gtgagcttgc tggatcggat tgtggttgaa tcctatggga cggaaatgcc cttgaaccag
     1861 gttggttcaa tcaatgctcc agaacctgcc atgctggtcg ttcaaccctg ggatccatcc
     1921 ctgattggcg ctatcgaacg ggccatccgc agctcggatt tagggttgaa cccttcaaat
     1981 gatgggaaag tcgtccggat ccccattccg ccgctgacgc aggatcgtcg gaagcagctt
     2041 gccaaagcgg tgcgcgatac ggctgaagaa catcgcgccg ggttgcgcaa tatccgccgg
     2101 gatgccaatg accacctgaa aaagctgttg aa
//
LOCUS       DAOQDO010001130         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49628_length_2131_cov_2.895472, whole genome shotgun sequence.
ACCESSION   DAOQDO010001130 DAOQDO010000000
VERSION     DAOQDO010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49628_length_2131_cov_2.895472"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..954)
                     /locus_tag="PKZ53_16580"
     CDS             complement(64..954)
                     /locus_tag="PKZ53_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ42106.1"
                     /translation="MEFKKVGVLGCGLMGSGIAQVAASAGFETVVVEADQKALEKGMA
                     GIQKSLAKFVEKGTLTAEAKDEILGRLSSSLELSALADCDIIIEAIIENLPIKHEIFK
                     KLDELCKPETVFASNTSSLSVTQMMTVTSPERQRRFVGVHFFNPVPLMKLVEVVKTIL
                     TDPEIFDAVNDFGKKLGKVTVRTSDRTGFIVNRLLVPYMLDAIRALEEGVGTIEDIDN
                     AMKLGCGYPMGPFTLTDFVGVDTPYYIAQIMFDEFREKRFAPPPLLTRMVAAKLYGRK
                     TGRGFYDYADPKNPKPMNLV"
     gene            complement(1104..2051)
                     /locus_tag="PKZ53_16585"
     CDS             complement(1104..2051)
                     /locus_tag="PKZ53_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ42107.1"
                     /translation="MNIHKLTVPTPFYVGPVNVYLIDQDPLTLVDVGPNTDEAWNALQ
                     AELRRVGREVRDLRRILISHAHTDHFGLAGRVQEVSGARVFIHDWDAPRLKRDPDYTC
                     HNTLLHRAGVPNEVTQGFKAGYESVKELAYHLLDVDTLKDEDEILFQNSALRVVHTPG
                     HTPGSICLLRESTRHLLAADTLLKHITPNPTLHADPVDEQRRFPSLSEYLCSLARIKE
                     MAPTYIQTGHGDDIADYSEHFHTLVHYTDRRQQKVIQFVQQKQSVTAWELSRYLFPNT
                     KDVHIYLGVSEAQAHLDLAVAEGKLQLEKRGPVDFFRPA"
BASE COUNT          520 a          519 c          546 g          546 t
ORIGIN      
        1 tgtttcttca ttcttcattc ttcattcttc attcttcatt cttcattctt cattcaccac
       61 tccttagacc aaattcatcg gcttcggatt ttttggatcg gcatagtcat aaaatccgcg
      121 accggttttc cgcccataca gtttggcagc gaccatccgg gtgaggagcg gcggtggcgc
      181 aaaccgcttt tcacggaatt cgtcaaacat aatctgggca atgtagtagg gggtatcaac
      241 gccgacgaaa tccgtcaatg taaacggtcc catcggatag ccacagccaa gcttcatggc
      301 attgtcaata tcttcaattg tgccaacgcc ttcttcgagg gcgcgaatgg cgtccagcat
      361 atacggaacg agtaaccggt tgacaataaa gccagttcga tcactggtgc gcaccgtgac
      421 tttgcccagt tttttgccga aatcattcac ggcatcaaag atttcagggt cagtcagaat
      481 cgttttgacg acttccacca gtttcatcaa cggcaccggg ttaaagaagt gcacgccgac
      541 aaaccgacgt tgccgctcag ggctggttac cgtcatcatt tgagtgactg acagcgacga
      601 cgtattgctg gcaaacacgg tttctggttt gcacagttca tcaagctttt tgaatatttc
      661 atgcttgatg ggaagatttt caatgatggc ttcgatgatg atgtcacagt cggccagggc
      721 tgacagttcg aggctgctgg aaagccgtcc cagaatttcg tctttggctt cggcggtcag
      781 tgtgcctttt tcgacgaatt tggcgagtga tttctgaatg ccggccatgc ccttttcgag
      841 tgctttttgg tcagcttcga ccaccacggt ttcaaatccg gcgctggcag ccacctgggc
      901 aatgccagaa cccatcaatc cacatccaag aacacctact tttttaaatt ccataaggta
      961 tgagaacctc caacggtaaa atattaagga gcaacgggaa agcgaaataa tgaaatagcg
     1021 aaaagagagc aaatggtgag tcgcgagtcg tcaatccagg aaagtgagaa gaaaattcat
     1081 cctgtcattt gttatctgtc atctcaagcc gggcggaaga aatcaaccgg gccgcgtttt
     1141 tcaagctgga gtttgccttc ggcaactgcc agatcaaggt gggcctgggc ttcggaaacg
     1201 ccaaggtaga tgtgaacatc ctttgtattt ggaaagagat aacgcgaaag ctcccaggcg
     1261 gtgaccgatt gtttttgctg gacaaactga atcactttct gctgtcggcg atcagtgtaa
     1321 tgcaccagcg tatgaaaatg ctcggaataa tcagcaatat catcgccatg accggtctgg
     1381 atataggttg gggccatctc cttgatgcgc gccagcgaac ataaatactc ggaaagcgat
     1441 ggaaagcgac gctgttcgtc aactggatcc gcgtgtagcg tcggattggg tgtgatgtgt
     1501 ttcagcaggg tatctgccgc cagcaaatgc cgggtgcttt cgcgcaacag acaaatgctg
     1561 ccaggggtat gccccggcgt atgcaccacg cgtagcgccg aattttggaa caggatttcg
     1621 tcttcgtctt tgagcgtgtc tacgtcaagc aggtgatagg ccagttcttt aaccgattca
     1681 tatccagctt taaacccttg agtgacttca tttggaacac ccgcccggtg gagcaacgtg
     1741 ttatggcagg tgtagtctgg atcgcgtttc aggcgtggcg catcccaatc gtgaataaag
     1801 acgcgtgcgc cagagacttc ctgaactctg ccagccagcc caaaatggtc ggtatgagca
     1861 tgtgaaataa gaatgcgccg gaggtcacgg acttcgcgac ctacccggcg cagttcagcc
     1921 tgcagggcat tccaggcttc gtcggtattg ggaccgacat ccaccaacgt caatgggtcc
     1981 tggtcaatca ggtagacatt caccggaccg acataaaatg gcgttggaac ggtaagtttg
     2041 tggatattca ttggcatttt ggtttgtcgt cagtcgtcag tagaccaaac ccaggtactt
     2101 tcgtctgatg atttcctgga aagaattgtt c
//
LOCUS       DAOQDO010001131         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49676_length_2130_cov_2.424096, whole genome shotgun sequence.
ACCESSION   DAOQDO010001131 DAOQDO010000000
VERSION     DAOQDO010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49676_length_2130_cov_2.424096"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>2130
                     /locus_tag="PKZ53_16590"
     CDS             36..>2130
                     /locus_tag="PKZ53_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNJ42108.1"
                     /translation="MPISSTTHHLPALFNDLATDPAGQAFLSQLREQRRVIVLSGLIG
                     SARALAIALIQSISKKRVAYVLPTSEDIEAIEADIRYFTQLVNGLSPQSEEDDGVAVF
                     PVIEGGPYQSMPPHPEVLEARALTLTHMAEGKGRVYLLPARALVERILEQSKLDLVSL
                     TLDINEEYPLDDIVSMLGEVGYYRREPVVQVGEFSLRGGILDIFSPANPNPYRLEFFG
                     DTLDSIREFDIETQRSIKKCSQCQILPMNELPTVKSDFVAWSKAARQHWQDDRYQYQL
                     TPRLEAAERGDQFADWEYLMPLARPLEGSIFDYLKDVLLVIDEPVAIEQTLTSYQKKL
                     WQKFRGADDAGELVLSPEYFIHSPERLRGWVEQLRRVEFRVLGVEAATVDSEFVGALD
                     GIEVKTAEETPIFLFPLDRRQPDIRLTTQPARKFHGRIPQLVTALREMRQKGETALLV
                     MPSLGVAERIQDMLTEYEYPSVLLPDPQKMMTSSEIFARAACVVTVGTISGGFAFPCS
                     QLQFIVERELFDQAASPEEVRLLPPTKTRRKSSINSFLSDFRDLKIGDYVVHVDHGIG
                     RFHGLQQIQVDPRMPAREFVLLVYSDEAKLFVPVERLDLVQKYSSADGHTPTLDKLGG
                     IGWAKTKARVKRAMRNMAEELLKLYAERRLVQGYSFSPDGPWQKEFEEAFPYELTVDQ
                     TNSVGDIKADMESTLP"
BASE COUNT          472 a          570 c          577 g          511 t
ORIGIN      
        1 attcttcatt ctcaattctt cattcagtca aggttgtgcc tatttcatcc accactcatc
       61 atcttcccgc actgtttaat gatctggcga cggatccggc tggtcaggct tttctctctc
      121 agctccgtga gcaacggcgc gtgattgtgc tttccggttt gatcggaagc gcccgcgccc
      181 tggcgattgc cctcattcaa tccatctcca aaaaacgagt cgcctatgtg ttgccgactt
      241 cggaagacat tgaagcgatt gaagccgaca tccgctattt cacccagctt gtcaacgggt
      301 tgtcgcctca atctgaagaa gatgacggcg tggcggtgtt tccggtgatt gaaggtggtc
      361 cctatcagag catgccgccg catccggagg tgctcgaagc ccgtgctctg acgctgacgc
      421 acatggctga aggaaaaggc cgggtgtatt tgctgcctgc ccgggccctc gtcgaacgga
      481 ttttggaaca aagcaagctc gatctggtca gcctgacgct cgatatcaac gaagaatatc
      541 cgcttgatga cattgtttcg atgctcggtg aagtcggtta ttaccgtcgc gaaccagtgg
      601 ttcaggttgg ggaatttagc ctgcgcggcg gtatcctcga tattttttca cccgccaatc
      661 caaatccgta ccggttagag ttttttggcg acacgctgga ttcgattcgc gaatttgaca
      721 ttgaaacgca gcgctccatt aagaagtgca gccagtgtca aatattgccg atgaacgagt
      781 tacccaccgt caaaagcgat tttgtcgcct ggagcaaagc ggcgcgccag cattggcaag
      841 acgaccggta tcaatatcaa ctcacgccgc gacttgaagc cgctgaacgt ggtgaccagt
      901 ttgctgattg ggaatacctg atgccgctgg ccagaccgct ggaaggctcg atttttgatt
      961 acctgaagga tgttttgctg gtgattgacg agccggtggc gattgaacaa accctcacct
     1021 cctatcagaa aaagctctgg caaaagtttc gtggagctga tgatgcgggg gaactggtgc
     1081 tgtcgcccga gtatttcatt cattcaccag agcggttgcg gggatgggtc gagcaattgc
     1141 ggcgggttga gtttcgcgtt ctgggcgtag aagccgcaac ggtggatagc gaatttgtcg
     1201 gtgcgctcga tggcatcgaa gtcaaaactg ctgaggaaac gccgatcttt ctgtttccgc
     1261 ttgatcgccg ccagccagat attcggttga cgacccagcc agcccgcaaa ttccacggac
     1321 gcattcccca actcgtcacc gccttgcgcg aaatgcgcca gaaaggcgag acggccttgc
     1381 tcgtcatgcc ttcacttggg gtcgcggaac gcatccagga tatgctgacc gagtatgaat
     1441 atccgtcggt cttgttgcct gacccgcaga agatgatgac gtcgtccgag atttttgcac
     1501 gggcagcctg tgtcgtgacg gttggcacaa tcagcggcgg atttgccttt ccttgctctc
     1561 agttacagtt cattgtcgag cgcgaattat ttgaccaggc cgcatcgcct gaagaagtcc
     1621 gcctgctgcc accgaccaaa acccgccgta aaagctcgat caacagcttt ctctcagact
     1681 ttcgtgattt gaaaattggc gattatgtgg tgcatgttga ccacggaatt gggcgtttcc
     1741 acggcctgca acaaatccag gttgaccccc ggatgcccgc ccgcgagttt gtgctgctgg
     1801 tctattccga tgaagccaaa ctgtttgtgc cggtcgagcg cctggacctg gttcagaaat
     1861 attccagtgc ggatggccac acgccaacgc tcgacaaact gggtggcatt ggctgggcaa
     1921 agaccaaagc ccgagtcaag cgagccatgc gcaacatggc cgaagagtta ctgaaactct
     1981 atgccgaacg gcgactggtt caagggtact ctttttcacc ggacggaccc tggcaaaaag
     2041 aatttgaaga agcttttccc tacgaactga cggttgatca gaccaacagt gtcggtgata
     2101 tcaaagccga catggaatca accctgccca
//
LOCUS       DAOQDO010001132         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49680_length_2130_cov_2.313735, whole genome shotgun sequence.
ACCESSION   DAOQDO010001132 DAOQDO010000000
VERSION     DAOQDO010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49680_length_2130_cov_2.313735"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="PKZ53_16595"
     CDS             <1..2080
                     /locus_tag="PKZ53_16595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNJ42109.1"
                     /translation="NIFAKGSDAGLTRSPKIDEREPFNTLERLLTAQNFDGKCNITSV
                     KDVRGTDLPFTVVKTMMRVDLKTPLKSGDSFTFSVEWNHNINNQRILGGRGGYEFFPK
                     DGNYLYEIAQWFPRMAAYNDVSGWHHKQYLGAGEFTLEFGNYKVSITVPDDHVVAATG
                     VLQNPLEVLTKAQRDRWEQAKTAKSPVFIVTPDEAKANESNKPKGKKTWVFAAENVRD
                     FAFASSRKFVWDAQQHTVEGNKVMAMSFYPKEGMPLWDKYSTQAIIHTLNVYSRYSFG
                     YPYPTAISVNGPVGGMEYPMICFNGPRPEEDGTYSKMTKYGLISVIIHEVGHNFFPMI
                     VNSDERQWTWMDEGLNTFLQYLSQQEWEDNYPVGRGEPFTIIDYMTSSEQVPIMTNSE
                     SLLQFGNNAYAKPATALNILRETVLGRELFDFAFREYSRRWKFKRPMPADFFRTMEDA
                     SGVDLDWFWRGWFYTTDHTDISIENVKWLTINTQNPDVEKPLRKQERESQPETLSKTR
                     NKPLPKRVDQIPSLKDFYNEYDDLKITDRDRKEYAELVKTLSDREKELLNSGYNFYQV
                     EFKNIGGLVMPIILQIEYMDGTSEELRLPAEIWRYNNSTTYKLILTKKEIKSLTLDPH
                     LETADADLTNNYFPPRPVKSRFELFKEKRERPNPMRIEMLERKQGNGTDDPKKPAEPA
                     QTPEAPPKGK"
BASE COUNT          598 a          551 c          514 g          467 t
ORIGIN      
        1 aaacattttt gccaaagggt cggatgcggg cctgacccgc tcacccaaaa ttgacgaacg
       61 cgagccattt aacacccttg aacggctgct cacggctcaa aacttcgatg gaaaatgcaa
      121 cattacgtcc gtcaaagatg ttcgcggcac agacctgccg tttacggtcg taaaaaccat
      181 gatgcgggtt gacttaaaga caccgctcaa atcgggtgat tcattcacat tttcagttga
      241 gtggaatcac aacatcaata accagagaat cttaggcgga cgtggcgggt atgagttctt
      301 tcccaaggat ggaaattatc tctatgaaat cgcccagtgg ttcccacgca tggccgctta
      361 taacgatgtt tccggctggc atcacaagca atacctcggg gccggggaat ttacgctgga
      421 atttggaaac tacaaagtca gcatcaccgt gccggatgat catgtcgtgg cggccacggg
      481 tgtgctccaa aacccgctcg aagtcctgac caaagcccaa cgcgaccggt gggagcaggc
      541 caaaaccgca aaatctccag tatttatcgt cacacctgac gaagccaaag ccaacgaatc
      601 caacaaaccc aaaggcaaga agacctgggt gtttgccgcg gaaaacgtgc gtgattttgc
      661 ctttgcctcg tcgcgaaaat ttgtctggga tgcccagcaa catacggttg aaggcaataa
      721 agtgatggcc atgtcgtttt acccgaaaga aggcatgccg ctgtgggata aatactcgac
      781 tcaggccatc atccacacgc tcaatgtgta ttcgcgctat tcgtttggct atccctaccc
      841 caccgcgatt tcggtcaatg gtccggtggg cgggatggaa tatccgatga tttgctttaa
      901 tggtccacgt cctgaagaag acggcaccta ttccaaaatg accaaatacg ggctgatttc
      961 agtcatcatc cacgaagtcg ggcacaactt ctttccgatg attgtcaatt ctgacgaacg
     1021 ccagtggacc tggatggacg aaggactcaa caccttcctg cagtatctgt cgcaacagga
     1081 atgggaagac aattatccgg ttggtcgtgg cgaacccttt acgatcattg attatatgac
     1141 cagttccgaa caggtgccga ttatgaccaa ttcggaatcg ctgttgcagt ttggcaacaa
     1201 tgcctacgcc aaaccggcca cggcgctcaa cattctgcgg gaaacggtac ttggtcgcga
     1261 actctttgac tttgcgtttc gcgaatattc acggcggtgg aaatttaagc gcccgatgcc
     1321 ggctgatttc ttccgcacga tggaagatgc ttcaggtgtt gacctcgact ggttttggcg
     1381 tggatggttt tacaccaccg atcacaccga tatttccatc gaaaatgtca aatggctgac
     1441 gatcaacact caaaatccag acgtcgaaaa gccactccgc aagcaggaac gcgaaagcca
     1501 gcctgaaacc ctttctaaaa cgcgcaacaa accgctgccc aaacgggttg atcaaattcc
     1561 gtccctcaaa gacttttaca atgaatatga tgacctgaaa atcaccgacc gggatcgcaa
     1621 agagtatgct gagctggtga aaacgctttc ggaccgcgaa aaggaactgc tcaactccgg
     1681 gtataatttc tatcaggttg aatttaagaa tattggcggg ctcgtgatgc cgatcattct
     1741 ccagattgag tatatggatg ggaccagcga agaactccgc ctcccagccg aaatctggcg
     1801 ctataacaac tcgacgacct acaaactgat tctgacgaag aaggaaatca aatcgctaac
     1861 gcttgatcca cacctggaaa cagccgatgc cgatttgacg aacaactatt tccctccgcg
     1921 accggtgaaa tcacgctttg aattgtttaa ggaaaagcgt gagcgcccca acccgatgcg
     1981 aattgaaatg ctggaacgca aacaagggaa cggcactgac gatcccaaaa aacctgccga
     2041 gccagcccaa acgcctgagg cgccgccgaa agggaaatag ttctacttag ggttcagggt
     2101 tcagggtttg aaaaaacaaa aaggacctaa
//
LOCUS       DAOQDO010001133         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49700_length_2129_cov_3.661041, whole genome shotgun sequence.
ACCESSION   DAOQDO010001133 DAOQDO010000000
VERSION     DAOQDO010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49700_length_2129_cov_3.661041"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(428..1855)
                     /locus_tag="PKZ53_16600"
     CDS             complement(428..1855)
                     /locus_tag="PKZ53_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HNJ42110.1"
                     /translation="MPPVAPTVSVLTVVQNAAHSLEPFLKNVLSLSDQSFQIIELIVV
                     DNASTDHTSQLLATLSAQDLRVRCIRTSQVEETGTALQKAIVQGSGDITVVFPATFEY
                     SPDDLPALLKPVIAEGADAVLGSRFLPGSYRAVTNFQQTRYQQALTVLSNWLTDMTFS
                     DVQSPIKAINTQLLKSIPIESTDEWVHLELTFKLIKRRARVFEVPVRYLPHIHKLPQL
                     PDWWDWVSGVRAALRFKSTQRIFAADAYGSHILMNLEGAPRFNRWMGDVLRPHLGRRV
                     LEIGSGTGTLTDQFVPRDMYLASDINPNYIHFLRGFAAGRPFMQVREINASEPACFQG
                     LEEMFDTAVMVNVLEHVPDDLIALQNLWNSLQKGGKAVILVPQHPAIYGTLDEVLEHR
                     ERYTVEGLHQVMSKAGFEVEQIFDFNRVSVPGWWLNGKVLRKTSFSSFQLLILQILMP
                     VFRRIDRFFPWGGLSIIGVGVKKEG"
BASE COUNT          557 a          529 c          552 g          491 t
ORIGIN      
        1 gatggaaaat ctggagctga agacggcggg ctgaaaatac cagggctgag ggctgaagaa
       61 ggcgggctga agaagacggg cttggggctg aagaagacgg gctgaagact ttggcttggg
      121 gccatacacg ccaggttaag gaaacaacgt ccgatctgga cagggggaca aggggaccat
      181 tccactgacg gtgtgagcct caatgacatc cggttgggag cggggaacga agaccatctg
      241 ggtgtgtctc cttgtctcct tgtccccttg tctcctggtc cagatcgggt ttatcttgct
      301 tatcctggcg cttatgcccc ccagccctgg tattttcagc ccgccgtctt cagccccaag
      361 cccttagccc tggtattttc agcccgtctt cttcagcccc aagcccgtct tcttcagccc
      421 caagtcttca tccctctttc ttcaccccaa cgccgataat gctgagtcct ccccacggga
      481 agaagcggtc aattcggcgg aaaacaggca tcaggatttg caagataagg agttggaaag
      541 aggaaaacga ggtcttgcgt aaaactttcc cattgagcca ccagccaggc actgagaccc
      601 ggttgaagtc aaaaatctgc tccacttcaa atccagcttt cgacatgacc tgatgcagcc
      661 cttcgacggt atagcgttcg cgatgttcaa gcacttcgtc gagcgttccg taaatggccg
      721 ggtgttgtgg caccaaaatc acggctttcc cacctttttg caatgagttc cagagattct
      781 gaagtgcgat cagatcatca ggtacatgtt cgagtacatt gaccatcacc gctgtatcaa
      841 acatttcttc caatccctga aagcaggctg gttcgctggc attgatttcg cggacctgca
      901 taaacgggcg gcctgccgca aagcccctga gaaagtggat gtaattggga ttgatgtctg
      961 aggcgaggta catatcgcgc gggacaaact ggtccgtcaa cgtgccggtt ccagagccga
     1021 tttccagcac tctccgtcca agatgcgggc gtaacacatc gcccatccac cggttaaacc
     1081 gtggcgcccc ctccagattc atcaaaatat ggctgccata ggcatcagcg gcaaagattc
     1141 gttgagtgga cttgaaccgt agcgccgccc tcacaccact cacccaatcc caccaatcag
     1201 gaagttgagg caatttatga atgtgaggca gatacctgac cggtacttca aacactcgtg
     1261 cgcgacgttt gatgagtttg aaggtcagtt cgaggtggac ccattcatcc gtgctttcaa
     1321 ttggaattga tttgagtaac tgggtgttga tggccttaat tggcgattga acatccgaaa
     1381 aagtcatgtc tgtcagccag ttgctcaaca ccgtcagtgc ctgctgatag cgtgtctgtt
     1441 gaaaattggt gacggcccga taggaaccag gcagaaagcg ggatcccaag acagcatcgg
     1501 ccccttctgc aatgaccggt ttgagcaatg ccggaaggtc atcgggtgaa tattcaaacg
     1561 tggctggaaa taccaccgtg atgtcgccac ttccctggac gatggctttt tgcaaggccg
     1621 tgcctgtctc ttcaacctga ctggtgcgga tacacctgac tcgcaagtct tgggctgaca
     1681 aagtcgcaag taactggctg gtgtgatcag tgctggcgtt gtcaaccaca atgagttcta
     1741 tgatttggaa agattgatct gaaagggaga gaacgttctt gagaaacggc tcaagtgaat
     1801 gagccgcatt ctggacaacc gtgagcacac ttaccgtagg ggcaacaggt ggcataaagg
     1861 agatgaagaa tgaagaatga agaatgaaga atgaagaatg aagaaagtgg ttagtgatgc
     1921 cagttaaggg ttgaaaaaat tgttcgttga aaacaaagga cctatcaaaa tctcaaattt
     1981 tagacgttca atttactgcc tgtaaattgt tgattttgaa gtctcaattt caacccttaa
     2041 ttcgcatgag tggttagaaa tcactacatt cgaagaagaa ccaagaacca agcaccaagc
     2101 accaagcacc aagcaccaag caccaagaa
//
LOCUS       DAOQDO010001134         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49706_length_2129_cov_2.995178, whole genome shotgun sequence.
ACCESSION   DAOQDO010001134 DAOQDO010000000
VERSION     DAOQDO010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49706_length_2129_cov_2.995178"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PKZ53_16605"
     CDS             complement(<1..1052)
                     /locus_tag="PKZ53_16605"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ42111.1"
                     /translation="MPDTKAIDAEAQRLMKREHVQGLALAVIEGGKVVYVAAYGSRNV
                     ERNLPLTTDTVMYGASLTKTAFAYMLLQLVDEGRLTLDTPITKLLPRPLPEYEEFTDL
                     AGDERWRALTPRILLTHTSGFANFRWLEDDQKLRFHHTPGSRYGYSGEGFYILQLMLE
                     QGLGLDVGKEMQRRVFDRFGLTNTSMTWRPDFAKNLADGYGLDGKMEPHDERSSVSAA
                     GSMDTTIADQARLWAGIMRGDGLSAASRAELIRPQVPIMSAHQFPTLRTETNPRNAEI
                     GLAAGLGLVTFRDTTGPAWFKGGHNDWTGNMVIGLEHKQRCVVMLANDVRAERIYPEL
                     AQAILGNTKMPWNWEYR"
     gene            complement(1280..1891)
                     /locus_tag="PKZ53_16610"
     CDS             complement(1280..1891)
                     /locus_tag="PKZ53_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HNJ42112.1"
                     /translation="MILRREIETRFDGKAFQRPLIDAYPQGLRFRLSEGGSSIEQFLC
                     AHRKALEICSDIFEPNATLVVCIRFWSNASLFAGRTVISELHSAGIEFPQKRCVWSEP
                     VEEKRFADTTSPARWVTIAFEVPTTLLQNLLWCALVTDFVGIRPRPGCLVYLIDLKKE
                     ILVFPYDDRGMDVISPNQILLKALYAKYFQHLISESQVSRSQT"
     gene            complement(1875..>2129)
                     /locus_tag="PKZ53_16615"
     CDS             complement(1875..>2129)
                     /locus_tag="PKZ53_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42113.1"
                     /translation="RAAELLLCLPKDWLKKRIRGYLDRYRLQPGFEICTGLLSFCEDI
                     DPNFALELAQEAMSGQSDYEKEIGTFFYGRLFGQKNDPSS"
BASE COUNT          524 a          540 c          538 g          527 t
ORIGIN      
        1 cgatactccc agttccaggg cattttggtg tttccaagaa tcgcttgagc cagttctggg
       61 taaatccgtt cggcccggac atcattggcc agcatgacca cacagcgttg cttgtgttca
      121 agaccaatga ccatgttgcc ggtccagtcg ttgtggccac ccttaaacca tgctggcccg
      181 gtcgtatccc gaaatgtgac cagtcccagc ccggcggcaa gcccgatctc ggcgtttcgc
      241 gggttggttt ctgttctcag tgtcggaaat tgatgtgccg acataattgg cacctgaggg
      301 cgaatcagtt ccgcccgcga tgccgcactg agtccgtctc cacgcataat ccccgcccac
      361 agccgagcct gatcagcaat agtcgtgtcc attgaaccag cggcgctgac ggaactgcgt
      421 tcgtcgtgcg gctccatttt gccgtcgagt ccatagccat cagccaggtt tttcgcaaag
      481 tctggccgcc aggtcatact ggtgttggtc aggccaaacc ggtcaaacac acggcgctgc
      541 atttccttcc caacatccag tccaagtcct tgttcaagca taagttgcag aatataaaac
      601 ccttcgccgg agtagccata gcggctgcct ggagtgtggt gaaagcgcaa tttctggtcg
      661 tcttcaagcc accgaaagtt agcaaacccg gacgtatggg tgagcaaaat ccgtggtgtc
      721 agcgcccgcc agcgttcatc tccggccaga tcggtgaatt cttcatactc aggcagtggg
      781 cggggcagta gtttggtgat cggagtgtcg agcgtgagcc gcccttcatc cacaagctgt
      841 aacagcatat aggcaaaagc tgttttcgtc agcgacgcac catacataac cgtatcagtt
      901 gtgagcggga gatttcgttc gacattccgg cttccataag cggcgacata gacaactttg
      961 ccaccttcga tgaccgccag ggccaaacct tgaacatgct cacgcttcat cagccgttgg
     1021 gcttcggcgt caatggcttt tgtgtcaggc ataacctgtt ttttgggaga aattgcacaa
     1081 ccagcaagtg ccaaaaagag aagtaacggt atggccaatt tcatgtaaga tgtgttcctg
     1141 atgattgata aagggctggt accgtaatcg ggtttttgta ccagtatgga ctgaactata
     1201 aaataagcct ggaaaaataa taagttatgt gccgccttgc cttgagatat ggttactgcc
     1261 agcaatctga atactgacgc tatgtctgtg agcgtgatac ctgtgattcg ctgatcaaat
     1321 gctggaaata tttggcatac agtgctttaa gcagaatctg gttcgggctg atgacatcca
     1381 tcccacgatc atcataaggg aaaaccagaa tttctttctt caggtcaatc aggtacacca
     1441 ggcatccagg tcgggggcga atgcctacaa aatccgttac cagcgcgcac cagagtagat
     1501 tttgaagcaa tgtcgttgga acttcgaacg caatggtgac ccatcgggct gggctggttg
     1561 tatccgcaaa gcgtttctct tcaacgggtt cagaccagac acatcgtttt tggggaaact
     1621 caatcccggc tgaatgcaat tcagaaataa ccgtgcgacc ggcaaaaagt gaagcattgc
     1681 tccaaaatcg gatacagacc accagtgttg cattcggctc aaaaatatcg ctacaaattt
     1741 cgagtgcttt gcggtgtgcg cacaaaaatt gttcaatgga tgagcctcct tcagaaagtc
     1801 gaaagcggag accttgtgga taggcatcaa tcagcgggcg ctgaaacgct ttgccatcaa
     1861 atctggtttc gatctcacga cgaaggatca ttcttttgtc caaagagccg cccgtagaag
     1921 aaggtcccaa tttctttttc atagtctgat tgtcctgaca ttgcctcctg ggcgagttca
     1981 agcgcgaagt ttgggtcaat atcttcgcag aaactcagca atccggtaca aatttcaaag
     2041 cctggttgaa ggcgatatct atcgaggtaa ccccggattc tcttttttaa ccagtctttg
     2101 ggaaggcata aaaggagttc ggcagctcg
//
LOCUS       DAOQDO010001135         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49721_length_2129_cov_2.323047, whole genome shotgun sequence.
ACCESSION   DAOQDO010001135 DAOQDO010000000
VERSION     DAOQDO010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49721_length_2129_cov_2.323047"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..>2129)
                     /locus_tag="PKZ53_16620"
     CDS             complement(330..>2129)
                     /locus_tag="PKZ53_16620"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ42114.1"
                     /translation="GTSCGLTLLNQNLQVIGTVTTRDGLDSNLVKNGIGGPQIVLSHT
                     ESLSLFDPKTTIFTTLNKKDGLPPFKIDSLSIEDQKLKIQFAPRYLTRQHITITPTIY
                     DFETHHLAPSIVSQSIKAQFPQYLEKMPFMGGVIYGKQVINGNTFVFGSRGLMILPDE
                     KLPSFQIPEWPVRFSPQGISWLDVDNQFPQLPNLQPINSQPGNQPGKSQPDQPPGKIQ
                     TLSSNHPGQGHPSSGSGHTTSSQSKTLIGISRLQSPCDEPISLEYLRKLIENPFVIQQ
                     WNDQICIKALGKAVSTDPEMLELLLRSNPQTEHSLQGYKALLKQLFAHSDNQVLPVLH
                     QALNSTHLVVRRNAAYACAAAGDTSSIPLLIQTLDIESNFVRAGVIWALRELKAEQAA
                     ARLFNIYLESNCSSSRFSLPLRTGEMNHLFEKHYRELKAPTPQLGSSQSDNPDQLNKD
                     LISREYSWEETFRSICSHCPQTYFQLEIERGGYYGAVRAARCLENCSPLEIEQTKGIL
                     RPLAARGIIEAAVTLLILGDKNIQPTILTWLKEKRRTYADRDLILKDLLMELHRVKDK
                     SKLIFAREILTHYVDDPTIDYHTSGIIKGLIQP"
BASE COUNT          545 a          462 c          525 g          597 t
ORIGIN      
        1 tgagcttccc gttttatggg aaagctaaat tattgaaagg atgatatttc ctcgccaccc
       61 tgaaccctga accctgaacc ctaaatggta taagttcagg caatgaaccc gaaatcgggt
      121 gcccgttcct gggaaaccag ggtgtcagaa cgaaacacat gttgtgacta aatcagttca
      181 cacagggttg gaagactgat gattgatttc agattcagac catcgagttg ggccgtgatg
      241 agtcaccggc cagttcgacg gctttggatc aaccccgaac ccccaacatc cccctgaacc
      301 cgatgtggtt caatctcctg tggtttgatt tagggctgaa tcaacccttt aataattccg
      361 ctcgtgtggt aatcaatagt tggatcgtct acgtagtgag tcagaatctc acgggcaaaa
      421 atgagttttg atttatcctt cacacggtgt aactccatta gaaggtcctt cagtatcaga
      481 tcacggtcag catatgtccg tcttttctct ttcaaccagg tcaaaatagt aggttggatg
      541 ttcttatcgc ctaaaatcag gagggttact gcggcttcaa tgattcctct agccgcaaga
      601 gggcgcagaa tgcctttcgt ttgttcaatc tccaaaggag agcagttttc caggcatcgg
      661 gctgcccgaa cggcgccata gtatccacca cgttcgattt ccaattgaaa ataggtttgc
      721 ggacagtgag agcaaataga tcgaaaggtc tcttcccaac tatactcacg cgagatcaga
      781 tccttgttca attgatctgg gttgtcactc tgactggaac ccagttgagg tgtgggcgct
      841 ttcaattccc ggtaatgttt ttcaaacaaa tgattcattt caccagttcg aagtgggagt
      901 gaaaaccggg aactcgaaca attgctttca agatagatat tgaacaatct tgccgctgct
      961 tgctccgctt tgagttctct gagtgcccat atcactccag cccggacaaa attagactca
     1021 atgtccagtg tctggatcag cagtggaatt gaagatgtat caccagcggc agcacaggca
     1081 taggctgcgt ttcttctcac taccaaatga gttgagttca gggcctggtg aagcactggg
     1141 agtacctggt tatcgctgtg ggcaaaaagt tgtttcaaca aggctttata gccttgaagc
     1201 gagtgttcgg tttgagggtt ggatctgagc agaagttcaa gcatctccgg gtcagtggaa
     1261 accgctttcc caagggcctt gatacatatc tggtcattcc attgttgaat tacaaaaggg
     1321 ttttcgatca gttttcgaag atactctaac gaaatgggtt catcacaagg ggattgaagc
     1381 cgggatatcc cgatgagcgt cttcgactga ctcgaagtcg tgtgacctga tccagatgat
     1441 ggatgaccct gtcctggatg attggaactc agtgtctgaa tcttgcctgg cggttgatct
     1501 ggttgggatt ttcctggttg gtttcctggt tgggaattga tgggctgcaa attgggtaat
     1561 tgagggaatt gattgtccac gtctaaccac gaaattcctt gaggggaaaa tcgaactggc
     1621 cattctggga tctgaaatga tgggagtttc tcatctggca ggatcatcaa cccacgggag
     1681 ccaaatacaa aggtgttacc attgataacc tgtttcccgt aaatgacccc tcccataaat
     1741 ggcatctttt caagatattg tgggaattgc gccttgattg actggctcac aattgatggg
     1801 gccaggtggt gggtttcaaa atcataaatc gtcggagtta tggtgatgtg ctgccgggtc
     1861 aaatagcgag gagcaaactg gattttgagt ttttgatctt cgatggacaa tgaatcaatc
     1921 ttaaatggcg gaagtccatc tttcttgttt agcgtcgtaa aaatggttgt cttggggtca
     1981 aacaaagaaa gggattcagt atgactcagt acaatttgag gaccaccaat gccatttttg
     2041 actagatttg agtctaatcc atccctggtt gtgaccgtcc cgatgacctg cagattttga
     2101 ttcaacagcg tcaacccaca cgatgttcc
//
LOCUS       DAOQDO010001136         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49744_length_2128_cov_3.832610, whole genome shotgun sequence.
ACCESSION   DAOQDO010001136 DAOQDO010000000
VERSION     DAOQDO010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49744_length_2128_cov_3.832610"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKZ53_16625"
     CDS             <1..659
                     /locus_tag="PKZ53_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42115.1"
                     /translation="IDRLFDSVLLEPAEPEPLPLDSVSLLEKPVLHISPDDLVHLFLK
                     TFRLKRTRPLKTELALAEQWVAEYELTPDIAQTVVDFAHTESRRTNFQVQNLAGISQY
                     LDAALASNATPVAPIGDQPQQTSFLSFADPPSQTEDEKEQAYQILSSLPLAEQEHWAE
                     LAREELKRSNSAVFKRISTFSAEVQLETLLSNAAKFFTGEILGLKTRVKASKKPRIGS
                     "
     gene            complement(873..1793)
                     /locus_tag="PKZ53_16630"
     CDS             complement(873..1793)
                     /locus_tag="PKZ53_16630"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42116.1"
                     /translation="MPSWQNALLSFAIKSRLKRRPSDEMHHNVKRARSILSQMPGFAT
                     PPHRHHVSVHTHEDHHPVRGEWLKWKKKEHHSDKVLYFLHGGGYMACSTITHRPLTVG
                     LTQALRSDTFAIDYRLAPEHPFPAAVEDALTGYEWLLEQGISHKKMIIAGDSAGGGLT
                     LATMLAARDRGLPLPAASVCYSPWTDLAATGESLHTNDPHDVMFYGESIRKGRHIYLG
                     QAPPTNPLASPLYADLTGLPPLLLFASSTEVLLDDSTRLAHRAQAAGVHVDLQVWHGL
                     PHAWPIFHRLLPEGKKAVHYTAEFVKRHVV"
BASE COUNT          507 a          573 c          591 g          457 t
ORIGIN      
        1 cgattgaccg gttgtttgat tccgtgctgc tcgaacccgc cgaacccgaa cctttgcctc
       61 tggactccgt ctccttgctc gaaaaaccgg tccttcacat ctcgcccgac gacctggtgc
      121 acctgttttt gaaaaccttc cgcctgaagc gaacccgacc gctcaaaacc gagctggcac
      181 tggctgagca gtgggtggct gagtacgaac tcacccccga catcgcccaa accgtggttg
      241 attttgcgca caccgagtcc cggcgaacca atttccaggt ccagaacctg gctgggattt
      301 cgcagtacct cgacgcagca ctggcctcaa acgccactcc ggtggcaccg attggcgatc
      361 agccccagca aacttcgttt ctgtcgtttg cggatccccc gtcccagacg gaggatgaga
      421 aggaacaagc ctaccaaatc ctgagttcac tcccgctggc cgagcaggaa cactgggccg
      481 agctggcccg cgaggaactc aagcggtcaa acagtgcggt gttcaagcgt atttcgacct
      541 tttccgccga agttcagctc gaaaccctgc tcagcaacgc cgccaaattt ttcaccgggg
      601 agatacttgg gcttaaaacc cgcgtaaaag cgtcgaaaaa gccccggata ggcagttagt
      661 cattttttga aattgaagtg gctccacggt caaataaacg cgattacggg gcattagggg
      721 ggagcggtgg agggtgggat tttagagggt tccgggtttt tgaaagcagg gctgagggct
      781 gagggctagg ggttgagggt attaaacccg gtttcttccg ccccgagctt gcgagtcttc
      841 agccctcagc ccctagccct caaccctggt ttttagacca cgtgtcgttt gacgaattcc
      901 gccgtgtagt ggactgcttt tttgccttcc gggagcaggc gatgaaagat tggccaggcg
      961 tgcggcaacc cgtgccagac ctgcagatct acgtgaactc cggcggcttg agcccgatgc
     1021 gcgagccggg tggaatcgtc aagcaggact tcggtgctgc tggcaaagag caggagcggt
     1081 ggcaatcccg tcaggtcggc atagagcggt gacgccagcg gattggtcgg cggggcctgc
     1141 ccgaggtaga tgtgtcgccc tttgcgaatg gattcaccgt aaaacattac atcgtgcgga
     1201 tcattggtgt ggagtgattc gcccgtggcc gctaaatcag tccagggaga ataacatact
     1261 gatgctgcag gcagtggtaa tccgcgatca cgggccgcga gcattgttgc cagcgtcaat
     1321 cccccaccgg ctgagtcgcc ggcaataatc atcttcttgt gtgagattcc ctgttcaagc
     1381 aaccattcat aaccggtcag cgcgtcttcg accgcagccg gaaacgggtg ttctggtgcc
     1441 aaacggtaat caatcgcaaa cgtatctgaa cgaagtgcct gtgtcagtcc aaccgtcaat
     1501 gggcggtgag ttattgtcga gcaggccata tacccaccgc catgaagaaa atataacact
     1561 ttatccgaat gatgttcttt ctttttccac ttcagccatt caccacggac tgggtgatga
     1621 tcttcgtgag tatgaaccga aacatggtgg cgatgtggcg gtgtcgcaaa ccccggcatc
     1681 tgagacaaaa ttgaccgtgc ccgtttgaca ttgtggtgca tttcatctga ggggcggcgt
     1741 ttgagccggc ttttgatagc aaaactcaat agtgcattct gccagcttgg catagatgtt
     1801 ccctgatgct ttggtgaatt gaacgagtgc cggacagcat acacgaatca ggcaattttt
     1861 caccacagag ggcgcagagg aacacagagg aaaactgaat gaagaatgaa gaaatcatcg
     1921 ggttccgggt tccgggttcc gggtttctaa agggatgagg gatgaaggat gagggatgaa
     1981 aaaaatcacc cgatcatctt gtcagtcagt catcttgtca gggttccggg ttttcgaaac
     2041 caagcaccaa gcaccaagca ccaagcacca agcaccaagc accaagcacc aagaaccaag
     2101 aaccaagaac caagaaccaa gaaccaag
//
LOCUS       DAOQDO010001137         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49753_length_2128_cov_3.076700, whole genome shotgun sequence.
ACCESSION   DAOQDO010001137 DAOQDO010000000
VERSION     DAOQDO010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49753_length_2128_cov_3.076700"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>2128
                     /locus_tag="PKZ53_16635"
     CDS             40..>2128
                     /locus_tag="PKZ53_16635"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ42117.1"
                     /translation="MPGAVLTLTQTRDGYLWVGTFEGLTRFDGREFIQIDQSQFPLKD
                     QFGGVRSLLEDARGTLWIGTNGDGLYSYQNGIWGPRLTTADGLPGNIITSLCQDTAGR
                     LWIGTRMGLCWATIQGQSVTFHTVSAGSETPNDVRGVAIGRDGTIYFSSNNQLYSFHP
                     TQSPTHAILRETFDATINSLLVDSGGKLWIGTLGSGVRVWNQRVESQLTVREGMPDNN
                     LTVLCEDTDHNLWIGSNTKGLIRFSQGKVATFTESDGLSGVSACSAIQDQEGSLWVGL
                     YTGGLHQFQNGKFTTYTKTEGLPSDAAWVVFPDQDHSLWVGTNGGLSRMKDGQIRTYI
                     PNTEDPCVRTMLRDHNGTYWVGTRNAGLKTLDPERGTFQTFTTKNGLSGNYIRALCED
                     PSGTLWVATDHGLDQFQNGRFLPFSPFNELKFSMVLSLDADRTGSIWVGTDGNGVVRI
                     SNSEITQYTTRNGLTGDIIFGVYEDRDGTIWVTTNNGICRIQNNRTTAIGSRGGLPKQ
                     VYQILEDGTGNFWFSTNQGIFRVSISELNALADTLQSTLTLTSYNRLDGLKTEVGIAP
                     ATAWQDNQGRLWFPMRKGIVAIDPQQLPTNTNIPPVCIEEVLINGSPVDHLDSQVYRY
                     DQNNFEFHCSILSFQSAEKNLLKCKLEGFDSDWQEIKNRHTAYYTNIPPGTYTFQVIG
                     RNNDGVWNRTGTSI"
BASE COUNT          545 a          543 c          539 g          501 t
ORIGIN      
        1 caataccagc accagtcatg ggatcaacaa cagggtctgt tgccaggagc cgttttaacc
       61 cttacccaaa cccgagatgg ctatctctgg gtgggcacat ttgaaggatt gacccgcttt
      121 gatggacgcg agtttattca aattgaccag agccaatttc cgctcaagga tcaatttggg
      181 ggtgtcaggt cactgctcga agatgcccgg ggcacactct ggattggcac caatggcgat
      241 gggctctaca gctatcaaaa tggcatctgg gggccacgat taacgactgc cgatggattg
      301 ccgggcaaca ttattaccag tttgtgccag gacacagccg gacgattgtg gattggaacg
      361 cggatgggcc tctgctgggc caccatccag ggccaatctg tcacctttca cacggtttcc
      421 gctggttcag aaaccccaaa tgatgtgcgt ggcgtggcga taggacggga tggcaccatc
      481 tatttcagca gtaacaacca gctctattcc tttcatccga ctcagtcacc gactcacgcc
      541 attttgcgcg agacatttga cgctaccatc aatagtttgc tggttgattc gggcgggaaa
      601 ttgtggattg gcacacttgg aagtggtgta cgggtctgga accaaagggt tgaatcacaa
      661 ttgactgtcc gcgaaggaat gcccgataac aacctgaccg tgctctgcga agacactgac
      721 cataatctct ggatcggctc caacaccaaa gggctgattc gattttctca gggaaaagtg
      781 gctacgttta ctgaatccga tggtctttcc ggagtctcag cctgctcagc catacaagat
      841 caagaaggaa gcctctgggt tggactctac actggtgggt tgcatcagtt tcaaaacggg
      901 aaatttacga cgtacaccaa aaccgaagga cttccaagtg atgccgcctg ggttgtcttc
      961 ccagatcagg atcacagtct gtgggttggt accaatggcg gactcagtcg gatgaaagat
     1021 gggcaaattc gaacctatat tccaaatacc gaagatccct gtgtacgcac aatgctccgc
     1081 gatcacaacg gaacgtactg ggtcggcact cgcaatgccg gattaaagac gctcgaccca
     1141 gagcgcggca cattccagac attcaccacc aaaaatggcc tttccggcaa ttatattcgt
     1201 gctctgtgcg aagacccatc gggaacattg tgggttgcga ccgaccacgg gcttgaccag
     1261 tttcaaaacg gtcgctttct tcctttttca ccgtttaacg aactcaaatt cagcatggtg
     1321 ttgtccctgg atgcagatcg aaccggatca atctgggttg gaaccgatgg aaatggagtg
     1381 gtgcggattt ccaactccga gatcacacag tacacaaccc ggaacggcct caccggagat
     1441 attatttttg gcgtgtatga agatcgggat ggaaccatct gggttacgac caacaatggg
     1501 atctgccgca ttcaaaacaa ccgcacgact gcgattgggt cacggggcgg acttcccaaa
     1561 caggtgtacc agattttgga agatggcact ggcaatttct ggttttcgac caatcaggga
     1621 attttccgag tttcgatttc agaattaaat gcactggccg atacgctcca atcaacgctg
     1681 acactgacca gctacaatcg gctggatggc ctgaaaaccg aggttgggat tgcccctgca
     1741 accgcctggc aggacaacca gggccggctc tggtttccga tgcgaaaagg aatcgttgca
     1801 attgatcctc aacagttgcc caccaatacc aacattcccc cggtttgcat cgaggaagtt
     1861 ttgatcaatg gcagcccggt tgatcacctg gattcccagg tctaccgcta tgatcaaaac
     1921 aattttgaat ttcactgctc gatcctgagt tttcagtctg ctgagaaaaa tttgctcaaa
     1981 tgtaagttag aaggatttga ttctgattgg caggaaatta aaaaccggca cacggcctat
     2041 tataccaata tccctcctgg aacctatacc ttccaggtca ttgggcggaa caacgatgga
     2101 gtctggaacc gaacgggcac cagcattc
//
LOCUS       DAOQDO010001138         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49785_length_2127_cov_4.178571, whole genome shotgun sequence.
ACCESSION   DAOQDO010001138 DAOQDO010000000
VERSION     DAOQDO010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49785_length_2127_cov_4.178571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..430
                     /locus_tag="PKZ53_16640"
     CDS             5..430
                     /locus_tag="PKZ53_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNJ42118.1"
                     /translation="MKDVETSFLGTGWSFPPTFSKMTSSVVMVSDDLDIRQSLWVLFS
                     TSLGERIMLPEYGSLLWKMVFQNVTTTLMTQLEDAIRQAVLYWEARIDIVEIQIQADA
                     SQTGLVYIYLDYIIRQTNSRSNLVYPFYLNEATIPVEMP"
     gene            430..>2127
                     /locus_tag="PKZ53_16645"
     CDS             430..>2127
                     /locus_tag="PKZ53_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42119.1"
                     /translation="MDPHSGKIVINGAASQFDRWLQALDAGFAPVDGRPFPELLDFSV
                     RYAELIRFYDLQNQIDGNWVDFFISDPTMLAASIGATNLVAHEARYLNVKRRTAQTHS
                     FDEKLSALHQLFGVMLDLARRINMWHDGISQSPSVASTRLLSHTLTGLIETSLKPAVQ
                     KLKAFDLGAAEPEALGKPIGQVYSAFGPNWNLAAVRGDSSIYQGRTSLKKIDQVLTQL
                     NPIYYTFQDAISELQPLVQSDVEETLEDGNHKPQLALYIAFARLFQSAQETINTFSIR
                     YRNFYYFDVLRQTFRPPVPDQLYLTFALADDENVTSSEVPQGTLFPAGQDINDREILY
                     AADKNLLVTTAVLDQLLAIQKSFGPLYVADSTVNDTTRPKRTTHQVFSSITRLAAAIE
                     NQLSWPPFGSTQIEMTPVVTTELATLGFGLASQSLLLGGGTRTIRIQIQYPLEFQTTV
                     LNPQLEQIAAITGATPTDIFETLLEGAFQLFLSTTDGWLQVKTYQAKCKFTTDSDPNF
                     TLIISLPPEAPPIAPFSPEEPDPVTANPHPELPTCTAYLVQTPVLIGSDTQVWVYPLS
                     "
BASE COUNT          503 a          564 c          513 g          547 t
ORIGIN      
        1 gactatgaaa gatgtcgaaa cttcttttct tggaacgggt tggagctttc caccaacttt
       61 ttcgaaaatg acttcgagcg ttgtgatggt gagtgatgac ctggatatcc ggcagagcct
      121 ctgggtgctg ttttcgacct cgcttggcga gcgaattatg cttccggaat atggttcgct
      181 gctctggaaa atggtgtttc agaatgtcac caccacgctc atgacccagt tggaagacgc
      241 cattcgtcag gcagtcctgt actgggaagc gcgcattgac atcgttgaga ttcaaatcca
      301 ggccgatgcg agccagacgg ggctggttta tatctatctt gattacatca tccggcagac
      361 aaattcacgg agcaatctgg tctatccctt ttacctgaat gaagccacca ttcctgtgga
      421 gatgccttaa tggatccgca ttctggaaaa attgtcatca atggtgctgc gagccagttt
      481 gatcggtggt tgcaggcact tgatgccggt tttgcgccgg ttgacgggcg tccttttcca
      541 gaattactcg atttttcagt gcgatacgcg gaactcatcc ggttttatga tcttcagaac
      601 cagattgacg ggaactgggt tgattttttc atcagtgacc caaccatgct tgccgcgtcc
      661 attggtgcca ccaacctggt tgcccacgaa gccaggtacc tgaatgtaaa gcgccgcacg
      721 gcgcaaaccc attcctttga cgaaaagttg agcgccctgc accagctctt tggcgtgatg
      781 ctggatctgg cccgtcggat caatatgtgg catgacggca tcagccagag tccatccgtt
      841 gcttcaacca gattgctttc ccacacgctg accgggctga ttgaaacgtc cctcaagccg
      901 gcggtacaga aactcaaagc ctttgattta ggcgccgccg agccagaggc gctgggaaaa
      961 ccgattggtc aggtgtattc ggcatttggc ccaaactgga atctggctgc cgtcagaggg
     1021 gacagttcga tttatcaagg ccgtacgagc ctcaaaaaaa ttgatcaggt gctgactcag
     1081 cttaacccga tttattacac ctttcaggat gctatttccg aattgcaacc cctggtgcaa
     1141 agtgatgttg aagaaaccct tgaagatggc aaccacaaac cccagcttgc tctctacatt
     1201 gcctttgccc gtctttttca gtcagcccag gaaacaatca acacgttttc gatccggtat
     1261 cgcaattttt actattttga tgtgctccgg cagacgtttc ggccacctgt cccagatcag
     1321 ttgtacctga cctttgcgct ggctgatgat gaaaatgtta cgagcagcga agttccacag
     1381 gggacgctct tcccagccgg ccaggatatc aatgatcgcg aaatcctcta tgcggctgac
     1441 aaaaatttgc tggtgacaac tgccgtgtta gatcaacttc tggccatcca aaagtcgttt
     1501 ggaccactct atgttgctga ttccacagtg aatgacacaa ccagaccaaa acgaaccacg
     1561 catcaggtgt ttagctcaat cactcggctg gcagcggcaa ttgagaacca gctttcgtgg
     1621 ccaccctttg gaagcactca aattgaaatg actccggttg tcaccaccga actggcgacg
     1681 ctgggctttg gattggcaag ccaatctttg ctgcttggtg gtggaacgcg caccattcga
     1741 atccaaatcc agtacccgtt ggagtttcaa accacggtct tgaatccgca acttgagcaa
     1801 attgccgcca tcacgggtgc gacaccgacc gacatttttg aaaccttgct tgaaggcgcg
     1861 ttccaactgt ttctgtccac cacggatggc tggctacagg tcaaaaccta tcaggccaaa
     1921 tgtaagttca cgactgattc cgatccaaac tttacgttga tcatttcttt accgcctgaa
     1981 gccccgccaa ttgctccgtt ttcgcctgaa gaacccgacc cagtaacggc caatcctcat
     2041 cctgaattgc caacctgcac ggcgtatctg gttcaaacac cagttttgat tgggagtgat
     2101 acccaggttt gggtatatcc actgtca
//
LOCUS       DAOQDO010001139         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49800_length_2127_cov_2.701255, whole genome shotgun sequence.
ACCESSION   DAOQDO010001139 DAOQDO010000000
VERSION     DAOQDO010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49800_length_2127_cov_2.701255"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKZ53_16650"
     CDS             complement(<1..819)
                     /locus_tag="PKZ53_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ42120.1"
                     /translation="MFGVDRLFNQPKILNPVWRQAIDDYVNALAWSPDGKQLAVASVS
                     GPIFIFELESGRLRQALAGHGFGTTAIEWSRCGKFFASVGQDGKAKVWEIENERERHV
                     LNAGNAWVEHLAWSSHTSSGVPVFATAAGKKLNLWSAHGELLLAYPDHPSTISSLQWK
                     PNSRTPVLTSATYGQVALWAVDSTDPIQRLTWKGSILALHWSPDGKHIATGDQDSTVH
                     FWIVRTGQDLQMYGYPTKVRELSWDATGRYLATGGSPVITIWDCGGRGPEGSKPQ"
     gene            complement(807..>2127)
                     /locus_tag="PKZ53_16655"
     CDS             complement(807..>2127)
                     /locus_tag="PKZ53_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ42121.1"
                     /translation="FLGAGKTTLLNRILNENHGKRIAVIENEFGEVGVDQELVIGAEE
                     EIFEMNNGCICCTVRGDLIRILGNLMKRRDGFDFILVETTGMADPGPVAQTFFVDDEM
                     QTRLQLDGIVTVVDAKHVWHHIENQDEVKEQIAFADVILLNKVDLVPPDEVERLEARI
                     RHMNAAAKVYRTQDSVVEMDHILNLGGFNLQRALEVDPKFMEPEYPFEWAGVYALERG
                     HYELVLRDGPDPAMNATLLPLEISQSDKPDYAAALAEVQMDAVLTFSATEQPCQSGST
                     LVPGPTLSQLVLDRECSVFRIQIEQAGRYALFTEHHPAEFQATLRSPLGPVEPVLTHE
                     YKPDHEHDDEISSVGITTPGDLDPNKLNAWLREILTTKGQDIFRMKGVLSLCGDQRRF
                     VFQGVHMLFDGQPDRPWGTEPRHNSLIFIGRNLDRAALTEGFRQCLA"
BASE COUNT          493 a          582 c          552 g          500 t
ORIGIN      
        1 ctggggcttg ctaccttccg ggcctcgtcc gccgcaatcc caaatggtaa tcaccggact
       61 gccgccagtt gccagatagc ggcccgtggc atcccacgac aactcgcgga ctttggtcgg
      121 atagccgtac atttgcaaat cctgacctgt tcgcacaatc cagaagtgga cagttgaatc
      181 ctgatcaccc gtggcaatgt gttttccatc tggactccag tgcaaggcca gaatggaacc
      241 tttccaggtc aagcgctgaa tcgggtctgt cgaatcaacc gcccacaaag cgacctgacc
      301 ataggtggct gacgtcagca ctggcgttcg ggaattgggc ttccattgca ggctcgaaat
      361 agtactcgga tgatctggat aggccaggag caattccccg tgagccgacc acaggttgag
      421 tttttttcca gccgcggtgg caaaaacggg aacacctgag ctggtgtgag aactccaggc
      481 aaggtgttca acccaggcgt tcccagcatt gagaacgtgg cgctcgcgct cgttctcaat
      541 ttcccacact ttggcttttc catcctggcc gacactggcg aaaaattttc cacagcggct
      601 ccactcaatt gcggttgtcc caaatccgtg accagccagt gcctggcgga gccggcctga
      661 ctccagttca aaaataaaaa tcggaccact gactgatgcc actgccagtt gtttgccatc
      721 cggagaccac gcaagtgcat tgacataatc atcaatcgct tgtcgccaga cgggattgag
      781 tatttttggt tggttaaaaa gccggtctac gccaaacatt gtcgaaatcc ttcagttaaa
      841 gccgcacggt ccagattacg gccaataaaa atcagcgaat tatgccgtgg ttcggtaccc
      901 caggggcgat ctggctggcc atcaaagagc atatgcacac cctgaaaaac aaatcggcgc
      961 tggtctccgc ataaactgag cacacctttc atgcgaaaga tgtcctggcc tttcgtggtc
     1021 aggatttccc ggagccaggc gttgagtttg tttggatcca aatcgccggg cgtggtgatg
     1081 ccaaccgagg aaatttcgtc gtcgtgttcg tggtcaggtt tatattcgtg agtcaggacc
     1141 ggttcgaccg ggcccagcgg gctgcgaagc gttgcctgga attcggcggg atggtgttcg
     1201 gtaaacaggg cgtacctgcc ggcctgctca atttgaatcc gaaacaccga gcattcccga
     1261 tccagaacca gttgtgaaag ggttgggccg gggacaagtg tgctgcccga ttggcagggc
     1321 tgttcggttg cggaaaaggt caacaccgca tccatctgga cctctgccag tgctgccgca
     1381 taatccggtt tgtctgattg ggaaatctca agcggcaata atgtcgcatt catggccggg
     1441 tcggggccgt cccgcaaaac cagttcatag tgaccacgct ccagggcata caccccagcc
     1501 cattcaaacg ggtattccgg ctccataaat ttggggtcaa cttcaagcgc ccgttgcagg
     1561 ttaaagccgc ccaaattgag gatatggtcc atttcaacca ctgaatcctg ggttcgatag
     1621 actttggcgg cggcattcat atggcgaatg cgggcttcga gtcgttcgac ttcatctggt
     1681 gggaccaaat ccactttgtt gagcaaaatc acatcggcaa aggcaatttg ttctttgact
     1741 tcatcctgat tttcgatgtg atgccagaca tgtttggcat caaccaccgt gacgatgccg
     1801 tcgagttgga gtctggtttg catttcatca tccacaaaga aggtctgggc aaccggtcct
     1861 ggatcagcca tgccagtggt ttcaaccagg atgaaatcaa aaccgtcgcg gcgtttcatc
     1921 aaattgccaa ggatgcggat caggtctccg cgaacggtgc agcaaatgca cccattgttc
     1981 atctcgaaga tttcttcttc cgcaccaatg accagttcct gatcaacacc gacttcacca
     2041 aattcatttt caatcacagc aatgcgcttg ccgtggtttt cattcaggat tcgattcaac
     2101 agcgtggtct tccccgcgcc aagaaac
//
LOCUS       DAOQDO010001140         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49847_length_2125_cov_9.856039, whole genome shotgun sequence.
ACCESSION   DAOQDO010001140 DAOQDO010000000
VERSION     DAOQDO010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49847_length_2125_cov_9.856039"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PKZ53_16660"
     CDS             complement(<1..1356)
                     /locus_tag="PKZ53_16660"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNJ42122.1"
                     /translation="MLRQALVRKPWVWCFVTSILGIHLHLLSNSTPAFNLGDPTRTRQ
                     AATPTITLERVVGGLSSPLLATHARDTSGRLFLVEQPGRIRIFQNGTLLETPFLDISS
                     QLIFSGERGLLGLTFHPEFATNRRFFVNYTRRPDGATVIAEYQASASNPNLASNAERI
                     LLTIPQPFANHNGGMVEFGPDGFLYIGMGDGGSAGDPQNNAQNVDSLLGKFLRINVDS
                     GNPYSSPGDNPFFGSTRGRDEIYAVGLRNPFRFSFDRKTGELYAGDVGQNRLEEIDII
                     TRGGNYGWRVMEASDCFNPSTNCNRTGLTLPIAEYDHSVGCSVTGGYVYRGSRFPSLD
                     GIYFYGDYCSGVIFGYRNGRSFEVRAPDSNLNITSFGEDSEGELYVISQGGEVFRILG
                     PQNEPCSLTCPNDITVTDEDGNGSEVVTFSAPQTSGNCGTVTSTPASGSTFAVGTTTV
                     RS"
     gene            complement(1593..1895)
                     /locus_tag="PKZ53_16665"
     CDS             complement(1593..1895)
                     /locus_tag="PKZ53_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42123.1"
                     /translation="MGTDQKKFTEYRVGDEVSFATPNGMLYGEVVRIVTAGENGAVEI
                     EYEDGRRETKRMRDHAMRLLRRRTGVSELEEQRGPRTRSKDDEIQEVRRSDQRRRW"
     gene            1935..>2125
                     /locus_tag="PKZ53_16670"
     CDS             1935..>2125
                     /locus_tag="PKZ53_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42124.1"
                     /translation="MKNEECIWCLVPGTWCLGINTFEEEPRTKNQEPRTKNQEPRTKN
                     QIVSSFFILHSSFFILHSS"
BASE COUNT          523 a          537 c          555 g          510 t
ORIGIN      
        1 actcctgacg gtcgtggtgc cgactgcaaa cgttgatcca gaggctggcg tcgaggttac
       61 ggttccgcag ttgccgctgg tctgcggggc tgagaatgtc accacttccg agccattgcc
      121 gtcttcatcc gtaaccgtga tgtcattggg acaggtcagg gagcagggtt cattttgcgg
      181 gcccagaatg cgaaacactt cgccgccctg gctgatgaca tacagttcac cctcggaatc
      241 ttcgccaaac gacgtgatat tgaggttgct gtcaggggcg cggacttcaa acgaccgtcc
      301 gtttcgatag ccgaaaatca cgccggaaca ataatcgcca taaaaataga tgccatcgag
      361 tgatggaaac cggcttccgc gatagacata gccgccagtg accgaacagc caaccgaatg
      421 atcatattcg gctatcggca gcgtcaggcc cgtgcggtta cagttggttg acgggttaaa
      481 acaatccgag gcttccatga ctcgccagcc gtagttccca cccctggtga tgatatcaat
      541 ttcttcaagg cgattttggc cgacgtctcc ggcataaagt tcgccggttt tccgatcaaa
      601 agagaaccgg aaaggattgc gcagccccac ggcataaatt tcatcccgcc cacgagttga
      661 cccaaagaag gggttatcgc ccggtgagct ataaggattt ccggaatcaa cgttgatgcg
      721 cagaaatttt ccaaggaggc tgtctacgtt ctgggcattg ttttgcgggt caccagccga
      781 tccgccgtcg cccatcccga tatagagaaa tccatccggc ccaaactcga ccatgccgcc
      841 attatgattg gcaaagggct gtgggatcgt gagcaggatg cgctcggcgt tgctggccag
      901 attagggttg ctggcactgg cctggtactc ggcaattacc gtggcgccat caggtcgccg
      961 ggtgtagttc acaaaaaaac ggcgattggt tgcaaattct ggatgaaatg tgagtccaag
     1021 gaggccgcgt tctccactaa aaatcaattg tgaggaaata tccagaaatg gtgtttccag
     1081 cagcgtccca ttttgaaaaa tacggatccg tccaggctgt tcgaccagaa acaaccggcc
     1141 actggtgtcg cgggcatgcg tggccaggag cgggcttgat aaccctccga ccacgcgttc
     1201 aagggtgatg gttggcgtgg ctgcctgacg cgtcctggtg gggtccccca gattgaaagc
     1261 gggcgttgaa tttgagagaa ggtgcaggtg aattccaagg atgctggtga caaaacacca
     1321 tacccacggc ttgcgaacca aagcctgacg taacatagga ttcctttcta caatgccagt
     1381 ttaaaccaac aaccggctga tgtagtgttg acaaatgttc gttttcggtg ggcagcttca
     1441 actgaagaat aaagaatgaa gaaaaagcta aatgatttat tgacaagaga tagcttattc
     1501 acaattctga atggcagtcg gcaatcgctt gacgtgtgtt tgagtcaaac tgaagcaatc
     1561 gggtttgctt tttttgggac cgccctgagt gactaccagc ggcggcgctg gtcgctccga
     1621 cgaacttcct ggatttcgtc gtctttgctg cgggtgcgtg gtccgcgttg ttcttccagt
     1681 tcgcttacgc cagttcgccg tcgaagcaac cgcatggcgt ggtcgcgcat gcgtttggtc
     1741 tcgcggcggc catcttcata ttcaatttcg acggctccgt tctcaccagc cgttacgatt
     1801 cgaaccactt cgccatagag cataccgttt ggcgtggcaa atgaaacttc gtctccgaca
     1861 cgatattcag taaatttttt ctgatcagta cccataaacc ctcctgatgt cgaatgaaga
     1921 attgagaatg aagaatgaag aatgaagaat gtatctggtg cttggtgcct ggtacctggt
     1981 gcttaggaat caatactttc gaagaagaac caagaaccaa gaaccaagaa ccaagaacca
     2041 agaaccaaga accaagaacc aagaaccaaa ttgtttcttc attcttcatt cttcattctt
     2101 cattcttcat tcttcattct tcatt
//
LOCUS       DAOQDO010001141         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49902_length_2124_cov_3.684389, whole genome shotgun sequence.
ACCESSION   DAOQDO010001141 DAOQDO010000000
VERSION     DAOQDO010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49902_length_2124_cov_3.684389"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..838)
                     /locus_tag="PKZ53_16675"
     CDS             complement(53..838)
                     /locus_tag="PKZ53_16675"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ42125.1"
                     /translation="MQPNRPDAEGVILDAELLLKYRMFDRALATLEGALADLPRSIPL
                     REKLCEICVDQGLKEKAIEHLLALSSLYLEGGKLDRANYVLMQAKRLNPQLSITARLN
                     AIKEAENPRPRQMMPQHSQPGLVMPGYAAPIPMAGMYGRPSVKVISGDLSSVSLFDVV
                     QVIENSRITGILGLHSPQVVGRIYFNSGQIADADTGNLRGVDAFRKFVDVNEGMFEVE
                     KSAVEFKQNINAMSNTNLILDVLRELDEERSGMTGAHDEGHVH"
     gene            complement(957..1331)
                     /gene="cutA"
                     /locus_tag="PKZ53_16680"
     CDS             complement(957..1331)
                     /gene="cutA"
                     /locus_tag="PKZ53_16680"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNJ42126.1"
                     /translation="MELHELRLVFITIDTVEAGRALAQHLVTNHLAACVNIIPAIESV
                     YVWEGSLHQDSEVLLIAKTTAQAFPQLEAAVRANHPYTTPEILAFPVSQAFQGYADWV
                     ISSVTPPSGDTPTLSATDPSGQ"
     gene            1773..>2124
                     /locus_tag="PKZ53_16685"
     CDS             1773..>2124
                     /locus_tag="PKZ53_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42127.1"
                     /translation="MNRVATELREPGRPRLQLHRILNESAQTLLTVPEFAVCRSGQVR
                     RNLLWGALETLADQTPTDNSGAQVAITTFEAIEHQVEKVFPSRISGKAKIKSVDTPSP
                     KPQAPSPGLWIVSGI"
BASE COUNT          566 a          498 c          519 g          541 t
ORIGIN      
        1 tcttcattct caattcttca ttcttcattc tcaattcttc attcttcatt cttcaatgaa
       61 catggccttc atcgtgggcg ccggtcattc ccgagcgctc ttcatcaagt tcgcgcaaca
      121 catccagaat caagttggtg ttgctcatgg cattgatgtt ttgcttaaac tcgaccgctg
      181 acttttcaac ttcaaacatt ccttcgttga catcaacaaa cttccggaag gcatcaaccc
      241 cacgcaggtt cccggtgtcg gcatcggcaa tctggccgct gttgaaataa atacgcccca
      301 cgacttgagg cgagtggagt ccaagaatac cggtaattcg gctattctca atgacttgaa
      361 caacatcaaa gagactgacg ctgctcaggt caccagaaat aactttgaca cttgggcgac
      421 catacatccc agccatggga atcggcgccg catatcctgg catcactaat cccggttggg
      481 agtgttgggg catcatttgg cgtggacgcg gattttcagc ttctttgatg gcattgagtc
      541 gagcggtaat ggacagttgc gggttcaggc gcttggcctg catcaagaca tagtttgctc
      601 ggtccagttt cccaccttcc agatagaggg atgagagcgc gagcaagtgc tcaatggcct
      661 tttcttttaa accctggtca acacagattt cgcacagttt ttcacgaagt ggaatactgc
      721 ggggcaagtc agccaatgca ccctcaagcg ttgccaaagc acggtcaaac attcgatatt
      781 tcaagaggag ttcagcgtca agaatgactc cttctgcatc tgggcgattg ggttgcatga
      841 aagttagtcc tctactcttc ttttgttgct ttctcagcgt gggcggggca aaaaacagat
      901 caatcttgaa aaatatggac cagtaaacct gggatggctg gtccagtggt cattgattat
      961 tgcccagatg ggtcagtggc gctcagtgtg ggggtgtccc cagagggtgg cgtgactgac
     1021 gaaattaccc aatcggcata accttgaaaa gcctgactta ccggaaaagc cagaatttct
     1081 ggggttgtgt atggatgatt tgcacgaacc gcagcctcaa gctgtggaaa ggcctgagcg
     1141 gtggttttgg caatcagcag cacttcagaa tcctggtgga gcgatccttc ccagacataa
     1201 actgattcaa tggcaggaat gatattgaca caagcggcaa ggtggttggt gacaaggtgt
     1261 tgtgccagtg cgcgtcctgc ttcaacggta tcaatggtga taaaaacaag tcgtaattcg
     1321 tgaagttcca tttgaagtta tgggtatttc cagaatgcac atcgcgctta acccaacggc
     1381 aagcctggta gaaccgttgg ctatcggctc aaaagccgcg tacccgtata ctgtgcgagt
     1441 gtgattgacc agggtgaatg gtcgttcaga gaatgagtgg ttgattctaa agtagaatca
     1501 gtcactcatc agtcgcatat tgaattgctt tgtgaaatgc gctgcaaaaa atttcgataa
     1561 aaatttggat ccgcaggaga caaactgggc aaacgcattt taacaatgaa atcacgctga
     1621 agcacgaaag aaaagcagta aaaacagttc tcgccgcatt aaaaaacaat attcatatgg
     1681 tgcaagattt ttctggttac cttgaaaaag caagtggctc aaaattggag gtccagtctg
     1741 actttcactc agtccaagtc cttttggatc aaatgaatcg agttgcaaca gaactcagag
     1801 agccaggcag gccacggtta caactccatc ggattttaaa tgaatcagcc caaacattgc
     1861 tgactgtgcc tgaatttgct gtctgtcgaa gtgggcaggt caggcgcaat ttactttggg
     1921 gcgcgcttga aacactcgct gatcaaactc caaccgacaa ttctggagct caggtggcaa
     1981 tcacaacttt tgaggcgata gagcaccagg ttgaaaaggt tttcccttca cgaatttcag
     2041 gcaaagcaaa gatcaaaagc gttgataccc caagccccaa gccccaagcc ccaagcccag
     2101 gtctgtggat tgtctctgga attc
//
LOCUS       DAOQDO010001142         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49903_length_2124_cov_3.651039, whole genome shotgun sequence.
ACCESSION   DAOQDO010001142 DAOQDO010000000
VERSION     DAOQDO010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49903_length_2124_cov_3.651039"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1164)
                     /gene="pilM"
                     /locus_tag="PKZ53_16690"
     CDS             complement(103..1164)
                     /gene="pilM"
                     /locus_tag="PKZ53_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNJ42128.1"
                     /translation="MFGAKRSLVGVDIGSSAVKAVELKAHKGNFELVAIGHVPLLSDT
                     IVDGHIIDLNHVSDVISRIFTDQGIKTDQVGTSVSGHSVIVKKISVPYMSDEELQERI
                     NWEAEQHIPFDIADVNLDYDVLGRDPASGSMEVLLVACKRDKIAQYTQVIAAAGKKPV
                     FIDVDAYALQNAYELNYQPMPTTTVALLDVGASVTSINVVRGNSSLFTRDVSAGGNQY
                     TDLLQKELNLTFEQAEALKRGIPTDNGVQPEDAQPLIESVTDILAMEVQKTLDFWKAT
                     SQMGDSVQIDRILISGGSSKIRGLTAYFSERFGIPVERFNAFNSIKVNNKKFDEEYIK
                     ELAPNMAIAVGLACRCADL"
     gene            1819..>2124
                     /locus_tag="PKZ53_16695"
     CDS             1819..>2124
                     /locus_tag="PKZ53_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42129.1"
                     /translation="MSLVKQFRSQYGVAAILLLMSAALLGGFYLFQPPQHKPLGAILP
                     ASALLVCEFDQPVAALTSLQATLFWKNLVSATGASPLPAEITSHQWQSIPGLSFTEAG
                     "
BASE COUNT          552 a          523 c          492 g          557 t
ORIGIN      
        1 aaagccccct gacgtatgcc aggatttagt caattgcaaa agaaggggag ttgcccgaac
       61 ccaccctgaa ttgatttttc aggggttcga ttgaaaatcc cattacagat cagcacaccg
      121 acaggccaaa ccaacggcaa tagccatgtt aggtgccaac tccttgatgt attcctcatc
      181 aaatttctta ttgttgacct tgatgctgtt aaaggcgtta aatcgttcaa ccggaattcc
      241 aaaccgttct gagaaatagg ccgttagacc tcggattttg gaactgccac cagaaatcaa
      301 aatgcggtcg atttgaaccg aatcccccat ttgactggta gccttccaaa aatccagcgt
      361 tttttggact tccatcgcca gaatatcagt cactgactca atcaatggct gagcatcttc
      421 tggctgaaca ccattgtcgg tcgggatgcc gcgcttgagc gcttcagcct gttcaaaggt
      481 caggttcaat tccttttgca gtaaatcagt gtactgattc cctccggctg aaacgtcacg
      541 ggtaaataga gatgaattgc ctcggaccac attaatgctg gtcacactgg cacccacgtc
      601 aaggagcgca acggtggtgg tgggcatcgg ctggtagttg agttcgtaag cattctggag
      661 ggcatacgca tccacatcaa taaaaacggg ctttttcccc gctgccgcaa tgacctgcgt
      721 atactgagca atcttgtcgc gcttacaggc aaccaacaaa acttccatgc tcccgctggc
      781 tggatcacgt ccaaggacat cataatccag gttgacatct gcaatatcaa agggaatgtg
      841 ctgttcagct tcccagttga tgcgttcctg caactcttca tcactcatat acggaacaga
      901 gatttttttc acaattaccg agtggcctga aactgaggtt ccaacctgat cggttttgat
      961 cccttgatcg gtgaaaattc ggctaatcac atcactcaca tggtttaagt caataatgtg
     1021 accgtccaca atggtgtcag agagtaaagg aacgtgccca atggcaacca gctcaaaatt
     1081 gcccttgtga gccttgagtt ccactgcttt aaccgcgcta gagccaatat cgaccccgac
     1141 caagctacgt ttcgcaccaa acatagagta ccttttttct tgttggaaag actatagtga
     1201 gtccctcggt tcctagggca tcggcacgcg aaaggcgact gtcgatggtg tgccattttc
     1261 ttaagaaaaa atgacagcac ccggcaaaaa ggccggaaac tcctgaaaga acccgttgtg
     1321 aaacccctgg aagaccgctg ttctgaagaa aaactgaagg agaaggaaaa taaagcaggc
     1381 acagggagct gggtggccag caccactgtt caaaacctca cacctgtggc aaagagcttc
     1441 tttgtagagg cccgctcaga tttgatgaat ctgcacctgg ttacccagcc tggaactgat
     1501 gtgcttgatt accctggatg tatgatgatg cttgaatgag tgtgatttat gcgagtgatg
     1561 atatttagtg ctgctaatag gctgcccata attaccattc atccaagacg tacgtcaact
     1621 cttttttggt cacgacaaac ccggtggagg aaaagtgtgg attaatgagg tttttttccg
     1681 gtagaccagc cagagtgcta ttcaatcgtt tgctttcttt gatagtctca ggcgctattt
     1741 tattgcatga gccagagttg ttgataaggg ggttgggttc tccgacccgg tttttcctat
     1801 gaatggtagg tacgactgat gtcattggtt aaacaatttc gctctcaata tggtgtggca
     1861 gcgatattgc tcttgatgag tgcggcactg cttggtggat tttatctctt tcaaccaccg
     1921 caacacaaac cgcttggcgc aatccttccg gcatcagccc tgcttgtctg cgaatttgac
     1981 cagccggttg cagcactcac cagtctgcaa gccaccctct tttggaaaaa tctggtttca
     2041 gccacaggag catcgccact tccagcagaa atcacttccc accagtggca gtcaattcca
     2101 ggattatctt ttactgaagc ggga
//
LOCUS       DAOQDO010001143         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_49951_length_2123_cov_3.479207, whole genome shotgun sequence.
ACCESSION   DAOQDO010001143 DAOQDO010000000
VERSION     DAOQDO010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49951_length_2123_cov_3.479207"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKZ53_16700"
     CDS             complement(<1..712)
                     /locus_tag="PKZ53_16700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42130.1"
                     /translation="MTSRLYLLEPEGITGGRVYLQMVHEALSPHFPLSTLVDHKRTGG
                     PRRNRRLQHLWRTARSLPWFDPGAFFVWDDFSLWLFRKAMRRRTIFILHHYDPTIFRT
                     WQEEWLWEKTLRALTDCFEVVCVSLYWQTFLAERGIRSRVIYNAYDVNLIDTINQVSK
                     SDLKAKFNFPLDKPVVYVGRVKHTVEVQKLTEFIDHHPHYHFVTTGNKAVLNALMPNT
                     SLSYSEYLQFLRACDVAVF"
     gene            complement(768..1586)
                     /locus_tag="PKZ53_16705"
     CDS             complement(768..1586)
                     /locus_tag="PKZ53_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42131.1"
                     /translation="MQKKISVRQSSRFLTVLGSAVCLLVLVVPTWADVIRMKNGQAIT
                     GKIVSYSDRKFVIVYEGASAESRAIIALDDIDSVEFDGRPLPSGFSTSYGEKSPTRTD
                     TYTSPATTSTQPSDPGTSASTSGTTTTEPPVTREPDRTTPATNPEPSRPAVPPVIGFA
                     TVVAKEDWTYAKIDVEKGDRVTISADGTIQLGKGLEASPDGIESEDPGKLVADRPTGA
                     LIAVIGDDNDEFLFIGKSREFTAPRSGKLFLSVNENDLADNSGSYTVRIQVEKR"
     gene            1784..>2123
                     /locus_tag="PKZ53_16710"
     CDS             1784..>2123
                     /locus_tag="PKZ53_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42132.1"
                     /translation="MKTTHVDQTTRLQLVRCLEFPTRHRFADIQVWCLGLLVILFLGS
                     TVSAQTTRSKPPAFPTRELAEFHKTLSPLVKQALPNKDFGRIRRSTPTLSRLRTKILQ
                     SPLPPVRSEEE"
BASE COUNT          538 a          549 c          519 g          517 t
ORIGIN      
        1 gaaaaacagc gacatcacag gctcgcagaa actgcaaata ttctgaatat gaaaggctgg
       61 tgttgggcat cagggcgttc aatacggctt tgttcccggt ggtcacaaag tggtagtgag
      121 gatggtggtc aataaactcg gtcaattttt ggacttcaac cgtgtgtttg acacgtccaa
      181 cataaaccac tggcttgtcc agtggaaaat tgaattttgc cttgaggtcg cttttggaaa
      241 cctggttgat tgtgtcaatc aggttgacat cataggcatt gtaaatcacc cgtgatcgaa
      301 taccacgctc agccagaaag gtctgccagt agagagacac acacacaacc tcaaaacaat
      361 cggtcagtgc cctcagtgtt ttctcccata accattcctc ctgccaggta cgaaaaatcg
      421 ttgggtcata atggtggagg ataaaaatgg tccgccgccg catggccttg cgaaacaacc
      481 agaggctgaa atcatcccag acaaaaaacg cacctgggtc aaaccaggga agcgaccggg
      541 cggtacgcca taaatgctgc aaccgacggt tgcgacgtgg gccgccggtc cgcttatgat
      601 ccaccagtgt ggacagcgga aagtgtggag acagcgcctc gtgaaccatc tgcaaataga
      661 ctcttccgcc tgtaatccct tcaggttcaa gtaagtaaag gcgactcgtc atacattatt
      721 cagtcagcca gccagataaa tcggatggtc ggctgctcgc agtcgcacta tcgtttctca
      781 acttgaatac ggacagtata gctccctgag ttatcagcca ggtcattttc attgacactc
      841 aaaaagagtt ttccgctgcg gggggcagta aattcacgcg atttcccgat aaataaaaat
      901 tcatcattgt catcaccaat cacggcaatc aaggccccgg tcggacgatc agccacaagt
      961 tttccagggt cttcgctttc aattccatcc ggagaagctt cgagcccttt tccaagttga
     1021 attgtgccgt ctgccgaaat cgtcacccga tccccttttt caacatcaat tttggcatag
     1081 gtccaatctt ctttggcgac aaccgtggca aacccaatca cgggtggtac ggctggtcgt
     1141 gatggctcag gattggtggc tggggtcgtg cgatcaggtt cccgtgtgac tggcggttcc
     1201 gtggtcgtgg tgccagaggt tgaggcactg gtcccagggt cggaaggttg ggttgaagtc
     1261 gtggctggac tggtataggt atcggttcgg gtcggtgact tttctccgta agaggtgctg
     1321 aatcccgacg gtaacggacg accatcaaat tcaacgctgt caatgtcatc cagggcaata
     1381 attgctcgtg attcagccga agccccttca tagacaatga caaattttcg atcactgtag
     1441 ctgacgattt ttcccgtaat cgcttggccg tttttcattc gaatcacatc ggcccaggtt
     1501 ggaacaacca gcaccagcaa acatacggca ctgcccagaa cggtcaaaaa acgtgaggat
     1561 tgacgaacgg atatcttctt ctgcataagt agtgtgtacc tgcaacccaa ctgggctgca
     1621 atgactgcct ccggatggat gaaatcagca atgtgtggga cgtctttgag cgcacctcgt
     1681 tgggggtaca gccatcaaca cattgatcgg tgtggactgt cagactactt ttatcttccc
     1741 taaatttcaa cctgtcacag gtttcagttt ttagatccaa gccatgaaaa ccactcatgt
     1801 tgaccaaaca acacgtcttc aacttgttcg ctgcctggaa ttcccgacac gccaccggtt
     1861 tgccgacatc caggtttggt gcctgggatt gctggtaatc ttgtttttgg gatcaacggt
     1921 cagtgcccaa acaaccagat caaagccccc agctttcccg acccgagaac tggctgaatt
     1981 tcataaaacc ctttccccac tggtcaagca ggcattgcca aataaggatt ttggtcgaat
     2041 tcgccgcagt accccaacct tgagccggct gcgcacaaaa attctacaat ctccgctccc
     2101 gcctgtccgg agcgaagaag aac
//
LOCUS       DAOQDO010001144         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50102_length_2120_cov_2.493947, whole genome shotgun sequence.
ACCESSION   DAOQDO010001144 DAOQDO010000000
VERSION     DAOQDO010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50102_length_2120_cov_2.493947"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKZ53_16715"
     CDS             complement(<1..1057)
                     /locus_tag="PKZ53_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ42133.1"
                     /translation="MKKELSRRDFLKMASTAGFGALVISSTKAWGLDAITNPLAVYPD
                     RGWEAVYRDLWKYDSKYTFLCAPNDTHNCILNAHVRNGVVTRIGPTMRYGEAKDLAGN
                     GTTHRWDPRVCQKGLALTRRFYGDRRVNQCMVRAGFKRWYEAGFPRESDGKPPVEYFQ
                     RARDEWVRMSHDEAAKIVAAALKNIAETYTGEAGKERLKKQHYDEATIEATKGVGTQV
                     LKFRGGMPLLGITRVFGMYRMANSMALLDAHIRKVGPDQALGGRGFDNYSWHTDLPPG
                     HPMVTGQQTVEFDLNSVEHAKTVVVWGMNWITTKMPDAHWLTEARLKGTRIIVIACEY
                     SATATKGDDVLVVRPGTT"
     gene            complement(1054..>2120)
                     /locus_tag="PKZ53_16720"
     CDS             complement(1054..>2120)
                     /locus_tag="PKZ53_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ42134.1"
                     /translation="DWYQLHVQKNQQAADGPWKTSFGQINEADLAAIQMYLATRVGAP
                     KLIEAKATFLSLGCMGCHKVSGIGGDAGVDLSRSGERDPGQLNFTHIPGQPTFANWLA
                     EHFRSPIGIVPGSQMPMMRLSEAQIDQLTMYVLSLRRRELPGTYLPKDQVRVLRFSER
                     EFARDGATMYSALCSACHGAKGQGVRYPGLPPFPGITNPDFINLVSDEFLTATITSGR
                     PGRKMPAWGGSGTALNPEEIKAIVGYLRQVSATTPVAETTPPRWVKGNPVQGEKLFAA
                     NCSGCHGTKGEGNEGPALNNQVFLKSATDRFLVETITQGRRGTVMAGYSQSSTVRPAL
                     TPAEIESIVTFMRSWEGKKR"
BASE COUNT          428 a          606 c          593 g          493 t
ORIGIN      
        1 gtgtggttcc gggacgaacc accaggacgt catcgccttt ggtggccgtg gccgaatatt
       61 cacaggcaat cacgatgatt cgcgtgcctt taagacgggc ttcggtcagc cagtgggcat
      121 cgggcatctt ggtcgtgatc cagttcatgc cccaaaccac cacagttttg gcgtgttcga
      181 ccgaattcaa atcaaattcc actgtttgct ggcccgtaac cattgggtgg ccgggcggta
      241 aatcagtgtg ccacgaatag ttgtcaaacc cgcgcccgcc aagcgcctga tcggggccca
      301 ctttgcggat atgcgcatca agcagcgcca tcgaattggc catccggtac attccaaaca
      361 cgcgggtaat gccgagcaag ggcatcccac cacggaattt caaaacctgg gtcccgacgc
      421 ctttggtggc ctcgatggtg gcctcgtcat agtgctgttt tttgagccgt tctttgccag
      481 cttcgccggt gtaggtttca gcgatgtttt tcaacgctgc cgccacaatt ttggctgctt
      541 cgtcgtgcga catccgcacc cattcatcac gcgcccgctg aaagtattca acgggtggtt
      601 ttccatcaga ctcgcgggga aacccggctt cataccagcg tttgaacccg gcgcgaacca
      661 tacactggtt cacccggcga tcaccataaa accggcgcgt cagggccaga cctttctgac
      721 agacgcgtgg atcccagcgg tgggtggtgc cgttaccggc taaatctttc gcttcgccat
      781 agcgcatggt cggtccaatg cgcgtgacaa cgccgttgcg aacgtgagca ttgagaatgc
      841 agttgtgggt gtcgtttggg gcacagagga atgtgtactt tgaatcatac ttccagaggt
      901 cccgatagac ggcttcccat ccacggtcag ggtagaccgc cagcgggttg gtaatggcat
      961 ccagccccca cgctttggtg gaggagataa ccagggcgcc aaaccccgcc gttgaagcca
     1021 tcttcagaaa atcacgtcgt gaaagttcct ttttcatcgt tttttcccct cccaggagcg
     1081 cataaaagtc acgatggatt caatttcagc cggtgtcagc gccggtcgca ccgtggatga
     1141 ttgtgaatag cctgccatca ccgttccccg gcgcccctgg gtgatggttt caaccagaaa
     1201 gcgatcagtg gctgatttca ggaaaacctg attgttgagc gccgggcctt cgtttccttc
     1261 gcctttggtg ccgtggcagc cgctgcaatt ggcggcaaag agtttttcac cctggactgg
     1321 attgcctttc acccaccgtg gcggtgtcgt ttcggcaact ggagtcgtgg ccgaaacctg
     1381 acgcagatac cccacgatgg ccttgatttc ctcggggttc agtgccgtcc ctgacccgcc
     1441 ccaggccggc atttttcgtc ctggacgacc actggtgatc gtcgccgtca aaaattcatc
     1501 tgacaccagg ttgatgaaat ccgggttggt aatgcctgga aacggcggaa gccctggata
     1561 tcgaaccccc tgtcctttgg caccgtggca ggccgagcac aacgcgctgt acatcgtggc
     1621 cccatcgcgg gcaaattcgc gctcactaaa gcggagcacc cgcacctgat ctttgggcag
     1681 gtatgttccg ggcagttcgc gccgcctgag tgacagcaca tacattgtga gttgatcaat
     1741 ttgggcttct gaaagccgca tcatcggcat ttgcgaaccg ggaacaatgc caatcggtga
     1801 ccgaaagtgc tcagccagcc agttggcaaa cgttggctgg cctggaatat gggtgaaatt
     1861 gagttggcca gggtcgcgtt caccgcttcg cgagaggtca acgccggcgt cgcctccgat
     1921 gccgctcact ttatgacacc ccatgcagcc gagtgaaaga aacgtggcct tggcttcaat
     1981 caactttggt gctccgacgc gggttgccag atacatttga atggcagcaa ggtcggcttc
     2041 attgatctgg ccaaacgagg ttttccaggg accgtcggct gcctgttgat tcttttgaac
     2101 atggagttga taccagtctt
//
LOCUS       DAOQDO010001145         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50133_length_2119_cov_3.810078, whole genome shotgun sequence.
ACCESSION   DAOQDO010001145 DAOQDO010000000
VERSION     DAOQDO010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50133_length_2119_cov_3.810078"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKZ53_16725"
     CDS             complement(<1..518)
                     /locus_tag="PKZ53_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNJ42135.1"
                     /translation="MAKPSQLLKFPPGFLWGTATSSHQVEGFNTNNDWWEFEQQPGTI
                     HDGTHSGAACDHFARFREDFDLAASMHQNAHRLSLEWSRLEPEEGVWNEDAFEHYRAV
                     LQALRDRNITPMVTLHHFTNPKWLVAKGGWETPQIVKYFYRFAAEVARAFGDLVEYWV
                     TINEPMIYVVMG"
     gene            791..>2119
                     /locus_tag="PKZ53_16730"
     CDS             791..>2119
                     /locus_tag="PKZ53_16730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42136.1"
                     /translation="MIDSSQARLILELALQKGLIHQEQISLCTPTGYTEPEVWSARLK
                     EVVHKGILTEKIVQTLSDEVVAFRLLQTQAPDNTLLLAQSGSGTVTETTLAFQPAPEP
                     LHLASVSQLPTQMATPGATPHQSAPKSGINRELEFRQVGRYEIVRRIGEGGMGTVYEA
                     FDPLLKRPVALKFLIKDNPTLVQRLLMEGRSQAQIDHPHVCKVYEVGEFEGRYFIAMQ
                     YLPGQTLDKAMAGLSVEQKVKIMAEVADALQAAHKEGLIHRDIKPHNIIVGTGEDGLL
                     KPYVMDFGLARQVAASGMTVEGALLGTPYYMSPEQARGETDRLDRRTDIYSLGVTLYE
                     LLSGKVPFSQGSIMEVLLKVIQDEAPALRSLDSRIPVDLETIVMKCLAKEPQQRYDSA
                     RALQDDLLRFLAGEPIQGRPVSAWFRAKRLLYKHRVAASIVAAALGIGLFF"
BASE COUNT          495 a          542 c          578 g          504 t
ORIGIN      
        1 tatcccatca ccacataaat catcggctcg ttgattgtga cccagtattc aaccagatcg
       61 ccaaaggccc tggcgacttc ggcggcaaag cgataaaagt atttgacgat ttgcggggtt
      121 tcccatccgc ctttcgccac cagccatttg gggttggtaa aatgatggag ggtcaccatc
      181 ggggtaatat ttcgatctcg cagggcttga aggacagccc gatagtgctc aaaggcatct
      241 tcattccaga ccccttcttc aggctccagc cgactccatt caagcgaaag ccggtgggcg
      301 ttctggtgca ttgaagccgc aaggtcaaaa tcctcacgaa accgggcaaa atggtcgcag
      361 gctgctccgg aatgtgttcc atcgtgaatt gttcctggct gctgttcaaa ttcccaccag
      421 tcgttattgg tgttaaatcc ttcaacctga tgagatgacg tcgccgttcc ccacaaaaat
      481 ccaggcggaa acttcagaag ttgagatggt ttggccacgg tgggactcct ttttggcggt
      541 gcttggtact tggtgcttgg tgactggtac ttggaaagtg ttggttctga agagccaggc
      601 accaggcacc aaaccctgtt gataccggat tcatcatcag ggttcaagct gaaacagaag
      661 gacgaggtga cagaatgaca aggtgacaaa gtggccaaac tgcttcacta tttcaccact
      721 tcgctatttc actatttcac tatttcgcta tttcgctact tcgctatgtg ctgaaatgag
      781 gtttgaacca gtgattgatt ccagtcaagc caggctaatt cttgaacttg cattacagaa
      841 agggctgatt catcaagagc aaatcagtct gtgcaccccg accgggtaca cggaaccaga
      901 ggtctggtca gcgcggttga aagaagttgt gcacaaggga atcttaactg agaaaatagt
      961 acaaactctt tcagatgaag tggttgcatt tcgtttgctt caaacgcagg caccagacaa
     1021 cacccttttg ctggcccaat caggttcggg aactgtcacc gagacaacgc ttgcctttca
     1081 gccagctccc gagccgcttc atctggcgag tgtcagtcaa cttccaaccc aaatggccac
     1141 accgggtgcc acgccacatc agtcggcgcc aaaatccggg atcaatcgcg aacttgagtt
     1201 tcgacaggtt gggcgatatg aaattgtccg tcggattggc gaaggcggca tggggacggt
     1261 gtatgaggcc tttgacccgc tgttaaaacg cccggtggcg ctcaagtttt tgatcaaaga
     1321 caacccgaca cttgtccagc ggttgctgat ggaagggcgt tcccaggccc agattgatca
     1381 tccacacgtg tgcaaagtct atgaagttgg cgaatttgaa ggccggtatt ttatcgccat
     1441 gcagtatttg cccggtcaaa ccctggataa agccatggcc gggttgtcgg tggagcaaaa
     1501 ggttaaaatt atggccgaag tggccgacgc gctccaggcc gcccataaag aaggactgat
     1561 tcatcgcgac atcaagccgc acaatattat tgtcggcacc ggcgaagatg gattgctcaa
     1621 accctatgtg atggactttg gactggcccg gcaggtggcg gccagcggca tgaccgttga
     1681 aggggcgctg ttgggcactc cttattatat gtcacccgaa caggctcgcg gagaaaccga
     1741 ccgcctggac cgacgcaccg atatctacag cctgggggtg acactctatg aattgctctc
     1801 tggcaaggtt ccctttagcc aggggtccat tatggaggtg ttgctcaaag tcattcagga
     1861 tgaagcgccg gcgttgcgga gccttgattc gcgcattccg gttgatctgg aaacaattgt
     1921 catgaagtgc ctggcgaaag agccgcagca gcgctatgac tcagcccggg cgctccagga
     1981 tgacttgctc cgatttctgg ctggcgagcc gatccagggc cgacctgttt cagcctggtt
     2041 ccgggccaag aggttgctgt ataagcatcg cgtcgccgcc tcaattgtgg cggcggcgct
     2101 tgggattggg ttgttcttt
//
LOCUS       DAOQDO010001146         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50355_length_2114_cov_3.555124, whole genome shotgun sequence.
ACCESSION   DAOQDO010001146 DAOQDO010000000
VERSION     DAOQDO010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50355_length_2114_cov_3.555124"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1262
                     /locus_tag="PKZ53_16735"
     CDS             363..1262
                     /locus_tag="PKZ53_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42137.1"
                     /translation="MCTHFNVSARQEPEATRSLWDSEFLQKRPKATKPQPTKSKLTTI
                     RYKRKETIAAATSTSSKPESTQVVSSTPSRLTIGVTAWRLRPAKVSDDKAVRLLERTD
                     DTDSQRELTPERIDLDTPLEEGQKIRLSIEVPRSGYLYVIDREKYADGTVSEPYLIFP
                     VLRGGKLENNQVKAGVLVDIPGDDDPNPYFKVTRSGPTQVGEVLTLLIAPAPLAELKI
                     GNKIQKLPAEQFASWEKQWSTQVEMLELVDGSGNPITKEEKIAGTGGRILTRDDPPPQ
                     TLYSVTCQPNTTVMVPVTLKLGK"
     gene            1475..>2114
                     /locus_tag="PKZ53_16740"
     CDS             1475..>2114
                     /locus_tag="PKZ53_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ42138.1"
                     /translation="MSNNNEDKTLIQQSASHSTTGKNKGFNDQETIVDSGGGDQTVAY
                     NTSAGRKTAVEQGTLIHHSQGTDSNPMKHGIPTSQLVAPGMLVGEKYRIESELGRGGI
                     GVVYRALDIKVHNRPVVIKVLLSDSDDTDWLVKKFRHESEALSRIEHPGVVKVLDRGV
                     LENGKPFFVMEFIKGEILRARIPSGGMDFKTAADYLRQLGQALDAAHDEGVVH"
BASE COUNT          597 a          539 c          494 g          484 t
ORIGIN      
        1 cggatgacgg atgacggatg aaataaaacc agttcttcaa cccaatgtct tctgcccaac
       61 ttcttcagcc cttagcccaa tatcttcagc ccctagccct agcaagccct tagccccaaa
      121 ctgagaaaac ccttgatttt ttttgatcca tcttagagag tttgatggac atcccactca
      181 cttgattttt caacgattta caccttctca ctttattacc tatcaaaaac ctgaagcagc
      241 cccttggagc taacctcgaa acactcccag gacaaagaaa gggattcgac atgaggcatc
      301 tgtatttgaa ccgatttatg ttgagtagct tacgtggagt tttcattatt ctggtggttt
      361 gtatgtgtac ccacttcaat gtgagtgccc ggcaagaacc tgaagccacc cgctcgcttt
      421 gggattctga attcctccaa aaacggccca aagcgaccaa accacagccc acaaaatcaa
      481 aactcaccac cattcgctat aaacgcaaag aaaccattgc ggcagcaact tcaacatcca
      541 gcaaacctga atccacccag gtcgtcagca gcaccccgtc acggcttaca attggggtta
      601 ccgcctggag acttcgacca gccaaggttt cagacgacaa agcggtccga ctgctcgaac
      661 gaactgatga taccgacagc cagcgggaac tgacccccga gcgcattgac ctggatacgc
      721 cgcttgagga aggtcaaaaa atccgactca gcattgaagt gccacgctca gggtatctct
      781 atgtcattga tcgcgagaaa tatgccgacg gcacagtgag cgagccttat ttgatttttc
      841 cggttttgcg tggtggcaaa ctggaaaaca atcaggtcaa agcaggggta ctggttgata
      901 ttcccggcga tgacgatccc aacccatatt tcaaggtcac tcgcagtggc ccaacccagg
      961 ttggggaagt gctcacgctg ctgattgctc cggcaccact ggctgaactg aaaatcggca
     1021 acaaaatcca gaaacttccg gcggaacagt ttgcaagctg ggaaaaacag tggagcaccc
     1081 aggtggaaat gctcgaactc gttgatggct caggaaatcc gattaccaaa gaagaaaaaa
     1141 ttgccggaac cggtggccgc attctgaccc gtgacgatcc accaccccaa acactgtaca
     1201 gtgttacctg ccaacccaac accaccgtga tggtgccggt gacgctcaaa cttggaaaat
     1261 gaattgagaa tttgaaggga attaatgact tatgagttat gaagtatgaa gtatgaagta
     1321 accacccctt agttcatgtg tcaggtcatt ccaggttttt cagtggtttt gcttctttca
     1381 aaacctcaac ttgaacaaaa tcaaactctg aggtaaggat gttctttcac ccattcattg
     1441 ctcataggtc ataattcatc cctggacaaa tgccatgtca aataacaacg aagacaaaac
     1501 gctgatccag caatcagcga gtcacagcac tactggaaaa aacaaggggt ttaatgatca
     1561 ggaaaccatc gttgattctg gcggaggtga tcaaaccgta gcctataaca cctcggctgg
     1621 ccggaaaaca gccgtcgaac aaggcacttt gattcatcat agccagggaa ccgactcgaa
     1681 tccgatgaaa catggaatcc cgaccagcca gcttgtggcg ccaggcatgc tggttggtga
     1741 aaaataccgg attgaatctg aacttgggcg aggtggaatt ggggttgtct atcgcgccct
     1801 ggatattaaa gttcataatc gcccggtggt gatcaaagta ctgctgtctg attcagatga
     1861 taccgactgg ctggtgaaaa aattccgcca cgaaagcgaa gccctgtccc gaattgagca
     1921 tccaggcgtg gtgaaagttc tggaccgggg cgtgttggaa aacggaaagc cgttttttgt
     1981 gatggaattt atcaaaggcg agattctccg agcccgaatt ccatctggcg gcatggattt
     2041 taaaacggcg gctgactatt tgcgccagtt aggtcaggct ctggacgccg cccatgacga
     2101 aggagtcgtt cacc
//
LOCUS       DAOQDO010001147         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50361_length_2114_cov_3.021370, whole genome shotgun sequence.
ACCESSION   DAOQDO010001147 DAOQDO010000000
VERSION     DAOQDO010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50361_length_2114_cov_3.021370"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(459..>2114)
                     /locus_tag="PKZ53_16745"
     CDS             complement(459..>2114)
                     /locus_tag="PKZ53_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HNJ42139.1"
                     /translation="DKGWSLNAIYTKELLTRDVQAALWIFFAAVGCVLLIACVNIASL
                     LLNRAEDRRKEFAIRTALGASRWRTIQQMLVESLVLSVTGGVLGLGLAAWLLSWLVTQ
                     TAAPIPRLREVSLDLSVVGFTFGVTLAVALVFGLIPAIQGGIKNLNQWLKEGGEKGDT
                     AGRNWLRNSLVVVEIAFALVLLIGAGLMIRSLWNLQHVETGFQTTNIITGQLSLPWTR
                     FQESTAFYDRVLAQTSALPGVKSASVVNFMPMVDASTPRKFEIEGRPLPPDEAAVSQF
                     YTISPNWFQTLGIPVLQGRSFLPKDTAESQRVLVVNASFARHYFPNADPIGKRINTGW
                     KDQPLWAEIIGVVGDIRHQDLETAPKPAAYASYVQSPWPAMMLVVKTENDPKGLVNSL
                     RTAIASVDQAQPLFDIQTLDERVLKASAVQQFNMLVLTVFAGVALLLATIGIYGVVAY
                     SVGRRRREIGIRLALGAQKSHIFQMVLGYGLTLCAIGMVLGLAGAFGLTRFVSSLLYA
                     VSPTDQLTFVAISGILLAVSLVACLVPVRRALNVDPVETLRTE"
BASE COUNT          547 a          584 c          524 g          459 t
ORIGIN      
        1 gtaccgccgc cgaatcgtcg tcccggtgtc aatgctgatt gcctgcatcg cagtgtactg
       61 gacaatccag cgattgtctt tttgacaggt gacttgctgt gaatggtcag aaaagaacga
      121 tttcttttct gaaaaagaag tcacagagca gacagaagat aagcttagct ttttcaacaa
      181 cttctgagat ttcgaccaaa tataaaccga ggacattgtt ttgtggaaaa cgagcgttcc
      241 aaattgtgca gtcgagtcgc ttttatacca aattgcaatg gagtcctcgt attagaaaag
      301 agtcaggtaa ttcaatcaaa tgaagttagt gaactcctga cgacgatctc ctgacgacaa
      361 actggtacta cattggtgca ttgtaaagca tggggaacaa tgctttttga acgggttggg
      421 aaattcccaa ccctattttt tatgtctatt ttcaagtctt attcggtgcg cagggtttca
      481 accgggtcaa cgttgagtgc ccggcgtacc ggaaccaaac aggccacgag gctgaccgca
      541 agcaatattc cggaaatggc gacaaaggta agctgatcgg ttggactcac cgcataaagc
      601 aaactcgaca caaagcgggt caacccaaaa gcacccgcca gccccagcac catgccaatt
      661 gcgcacagtg tcaacccata ccccagaacc atttggaaga tatgggattt ctgagccccc
      721 aacgccagcc ggatgccgat ttcacgccgt cgccgaccaa ctgaataggc cacaacgcca
      781 taaatcccga tagttgccag caacagcgcc acaccagcaa acaccgtcag caccagcatg
      841 ttaaattgct gcacggcact ggctttgagc acccgttcgt ccagggtttg aatgtcaaag
      901 agcggctggg cctgatcaac actggcaatc gccgtccgga gcgaattgac cagccctttc
      961 ggatcatttt ctgttttgac caccagcatc atcgctggcc aggggctttg aacatacgac
     1021 gcataggcgg ctggtttggg cgccgtctcc aaatcctgat gacggatatc cccaacgaca
     1081 ccaataattt cggcccacaa tggctggtct ttccatccgg tattgatccg tttgccaatc
     1141 gggtctgcat tcggaaagta atgccgggca aaactggcat tcaccaccag cactcgctgg
     1201 gattcagccg tgtctttggg aagaaaactc cggccctgga gcacaggaat tccaagggtt
     1261 tgaaaccagt ttggggagat cgtgtaaaac tgcgagacag cggcttcgtc aggcggaagc
     1321 gggcgaccct cgatttcaaa ttttctcggc gtcgaagcat ccaccatcgg cataaagttg
     1381 acaacactgg ctgatttcac gcctggtaag gctgaggttt gcgccagcac ccggtcataa
     1441 aaagccgtac tttcctgaaa ccgggtccag ggcagtgaaa gctgacccgt gatgatgttt
     1501 gtggtttgaa agccggtttc gacgtgctgc aagttccaca agctgcggat catcaatccg
     1561 gcaccgatca ataacaccag agcaaacgcg atttcgacca cgaccaggct gttccgcaac
     1621 cagttccgcc cggcggtgtc gcctttttcg ccgccttctt tcaaccactg gttgaggttc
     1681 ttgatgccgc cttgaatggc aggaatcaat ccaaaaacca gcgcaaccgc cagcgtcaca
     1741 ccaaaagtaa agcccaccac actcaggtca agggatactt cgcgaagacg tgggattggg
     1801 gcggcggttt gggttaccag ccagctcagc aaccaggctg ccagaccaag ccccagcaca
     1861 ccgccggtca ctgacaaaac caggctttcg accagcatct gttgaatcgt ccgccaccgg
     1921 ctggcgccaa gtgcggtccg gatggcaaat tccttgcggc ggtcttcagc ccgattgagc
     1981 aacagactgg caatgttgac acaggcaatc aacagtacac acccaacggc agcaaaaaag
     2041 atccacagtg ccgcctggac gtcacgcgtc agtaactctt tggtgtagat ggcatttaag
     2101 ctccatccct tgtc
//
LOCUS       DAOQDO010001148         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50464_length_2112_cov_2.267866, whole genome shotgun sequence.
ACCESSION   DAOQDO010001148 DAOQDO010000000
VERSION     DAOQDO010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50464_length_2112_cov_2.267866"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKZ53_16750"
     CDS             <1..239
                     /locus_tag="PKZ53_16750"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNJ42140.1"
                     /translation="LGQVQIAQETASVDLLALNEVLERLEKFDQRKAQITELRFFSGL
                     QNTEIAEVLHISETTVEREWRLAKAWLLKELNQS"
     gene            236..>2112
                     /locus_tag="PKZ53_16755"
     CDS             236..>2112
                     /locus_tag="PKZ53_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ42141.1"
                     /translation="MTPKELFEQAMSIFEQIVDLPPEDQPLALVKACQGNETLQREVA
                     ALLKSDQDGEHLTNAVHQQLRVVVENVSDPVVSIQVGQVLKDRYWIEKELAVGGIGRV
                     FVATDQLLHQRQVVVKVLLDTALQDPWLLKKFLHESEALAAIDHPGVVKIYDRGQVNQ
                     IPFLVMEFIEGESLGKILRKGALPFGQIRTYLGQICSALNAVHTQGIIHRDLKPDNIL
                     IKHNQDGTEHAILIDFGIARLTNPLSSSRTAIPRPAGTLPYMSAEILEDGEATVASDI
                     YALGVMTYEMITMHRPFTVKNKNQFAASFQLVEQQKKAEYIAPRVFCPDLSFEAEQVI
                     RKALMYPPSARYQDVFEFGQAITKALPVDPFPIPTSQDARFNESQITTQPDYVLMGNH
                     SISLATTVSLPDKVRQKDLPSLPVSAIPPRTAVKKTGLRWKLGGALAAIGILSIVGTI
                     IYNKNQVMLPVVKPRPQPTTPTNLASPLPQISIETQVINQGVTRTIPAGTELVKCRKN
                     DQLRFSILLEQAGYVYVLSESPRLDSNLNRRFTILYPGEQENQGSAFHLKGRTIVLPP
                     FNEPGLSFEGAQGTEKVWVIWSLESVPVLETLRTFANARFRGSIHQTNHLTAVSELLD
                     TP"
BASE COUNT          553 a          550 c          516 g          493 t
ORIGIN      
        1 cactgggtca ggtccagatt gcccaggaaa cagcctccgt ggatttactg gcattaaatg
       61 aagttttaga acgactggaa aaatttgacc agcgcaaggc tcaaatcact gagttacggt
      121 ttttcagcgg gctccaaaac acagaaatcg ctgaggtcct gcacatctcc gaaaccacgg
      181 ttgaacgcga atggcgactt gccaaagcct ggttattaaa ggagttaaac cagtcgtgac
      241 cccaaaggaa ctttttgaac aggcgatgtc aatttttgag cagatcgttg atctccctcc
      301 agaagaccag cctctggccc tggtcaaagc ctgtcaggga aatgaaaccc tgcagcggga
      361 ggttgcggca ctcctcaaaa gtgatcagga cggcgaacac ttaaccaacg ccgtgcacca
      421 acaactcaga gtggtggtcg agaacgtatc tgacccggtt gtctccattc aggtcggaca
      481 ggtcctcaaa gaccggtact ggattgaaaa agaactggcc gtaggtggta ttggccgggt
      541 ttttgtggcg acagaccagc tccttcatca acggcaagtt gtggttaaag tattgcttga
      601 tacggccctt caggatccct ggctgttgaa gaagtttctt cacgagagcg aggcactggc
      661 cgccattgac catccaggtg tggtgaaaat ttatgatcga gggcaggtca accaaattcc
      721 atttctggtt atggagttta tcgaaggtga atctttgggc aaaatcctgc gcaagggtgc
      781 tctccccttt gggcagattc gcacctacct gggacagatt tgttcagcgt tgaatgccgt
      841 tcacacccag ggaatcattc accgggatct caagccggac aatattttga tcaaacacaa
      901 ccaggatggc actgagcacg ccatcctgat tgattttgga atcgcacgac tgaccaatcc
      961 actgtcctcc tctcggacag ccatcccgcg tccggctgga accctaccgt acatgtcagc
     1021 cgaaatcctt gaagacggcg aagccacggt agccagcgat atatacgcct taggggtcat
     1081 gacctatgag atgattacaa tgcaccgccc ttttaccgtg aagaacaaaa accagtttgc
     1141 cgcctcattt caactggttg aacagcaaaa gaaggctgag tacatcgccc cccgtgtgtt
     1201 ttgcccagac ctttcttttg aggctgaaca ggtcatccgc aaggccctga tgtatcctcc
     1261 atctgctcga tatcaggatg tttttgagtt cggccaggcc ataacgaaag cattgccggt
     1321 ggatccgttt ccaattccaa ccagccagga cgctcgtttc aatgagtctc aaataacaac
     1381 ccaacccgac tacgtgctga tggggaacca ttctatttct ttggcgacca cggtttcgtt
     1441 gcctgacaag gtacgccaaa aggatttgcc atcactccct gtttcggcga ttccaccccg
     1501 tactgcggtg aaaaaaaccg ggttacggtg gaaacttgga ggggccctgg cagcaattgg
     1561 aatcctaagt atcgtgggaa ctataattta caataagaat caggtgatgt tgcccgttgt
     1621 gaaacccagg ccacaaccca caactccaac gaacctggcc agccccttgc cccaaatttc
     1681 aattgaaacc caggttatca atcagggagt gacccgtacg atcccggctg gtaccgaact
     1741 ggtgaaatgc aggaaaaacg accagctccg attctcaatc cttctcgaac aggcaggcta
     1801 tgtttatgtg ctgagtgaaa gcccgcgttt ggattcaaac cttaatcgtc gatttaccat
     1861 attgtatcca ggcgaacagg aaaatcaggg ctcagccttt cacctcaagg gcagaacaat
     1921 tgtcttgccc ccattcaacg aaccaggttt aagttttgaa ggggcacagg ggaccgaaaa
     1981 agtatgggtc atctggtcgc tggaaagtgt cccggtgtta gaaacccttc ggaccttcgc
     2041 gaatgctcgg tttcgaggaa gcattcacca aaccaatcac ttgactgcag tgagtgaatt
     2101 acttgatacc cc
//
LOCUS       DAOQDO010001149         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50486_length_2111_cov_4.130350, whole genome shotgun sequence.
ACCESSION   DAOQDO010001149 DAOQDO010000000
VERSION     DAOQDO010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50486_length_2111_cov_4.130350"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="PKZ53_16760"
     CDS             complement(<1..1995)
                     /locus_tag="PKZ53_16760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ42142.1"
                     /translation="MVLIPHLLAESNRLAELHRLHILDTPAEAVFDRVVAVAAQHFCV
                     PIAWLGFIDETRVWVKAHTGLNTTHLPLEPGLCVSALQTTSPTYSVSDTFTSSHAAEH
                     PLVLHPPGVRFYLAAPIITPEGHRIGVLAVADTSPREVSEADQAFLTTLATLVMDQLR
                     LRQAAVQARISKRLDPRDSQEHEEWFRSVVENTLDLITILDATGVIRYESPAIKWMLG
                     YSPEEVTGRSIFEFIHADDLPGVQEAFGNAVQRNISDQVIEFRLSRRDGAWRYVETRG
                     SRLLSEDRQVIGMVVISRDVTERKRTDQILQETNALLKAQLDATTSGVIGVDSQGKVI
                     VCNRRFLEMWELEELPDAAEGKRVEQLYTEGKLLAPEVFQATVDHLYAHPEEERHTGE
                     ELVLTNGRVLAWSTQPIRMSCGTIVGRAWDFTDITDQKETQKALHTAKEAAEASVRAK
                     SEFLANMSHEIRTPMNGIIGMTGLLLDTDLEPEQYEYLDIIQQSGEALLTIINDILDF
                     SKIEADKLELEKINFDLRRTIEGVVELFAESARRKDLELVCFIPQDVPGAVHGDPGRL
                     RQILINLVGNAIKFTEQGEVVVRVALSEDQSEQVVLRFDVTDTGIGITPEAQKRLFQS
                     FSQAELSTSRKYGGTGLGLAICKKLVELMDGEISVESEKGK"
BASE COUNT          460 a          563 c          588 g          500 t
ORIGIN      
        1 tttgcctttt tcgctctcaa cgctgatttc accatccatc aactcaacca gttttttaca
       61 aattgccagc ccgaggcctg tgcctccgta tttgcgcgag gtggacagct cagcctgaga
      121 gaatgattga aacagtcgtt tttgggcttc aggggtaatc ccaataccgg tgtcagtaac
      181 atcaaagcgc aggacgacct gctctgactg atcttcggag agtgccaccc ggaccacgac
      241 ttcaccttgt tcggtaaatt tgatcgcatt accgaccaga ttgatcaaaa tctggcgtaa
      301 gcgacctggg tcgccgtgca cggcacctgg gacatcctgg ggaatgaaac acaccagttc
      361 caggtctttg cggcgggctg attcggcaaa aagttcgacc acaccttcaa ttgtccggcg
      421 cagatcaaag ttgatttttt caagttcgag tttgtcggct tcaatcttcg agaaatccag
      481 aatgtcattg atgatcgtga gcagggcttc cccggattgt tgaatgatat ccagatactc
      541 gtattgttcc ggctctaaat cagtgtcaag cagcaggccc gtcatgccga taatgccatt
      601 catcggggtg cggatttcgt ggctcatatt ggccagaaac tcagatttgg cccgaaccga
      661 agcttcagcc gcttctttgg cagtgtgcag cgctttttgg gtttccttct ggtcggtgat
      721 atcagtgaaa tcccacgccc gtccgacaat ggtgccgcaa ctcatgcgaa tgggttgggt
      781 actccaggcc agaacccgac cgttggtcag cacaagttct tcacctgtgt gccgttcttc
      841 ttccggatgg gcatagagat ggtcaaccgt ggcttgaaat acctctggtg ccagtaactt
      901 gccttcggta taaagctgtt cgacgcgttt gccttcagcc gcatcgggga gttcttcgag
      961 ttcccacatt tccagaaacc gacgattgca aacgatgact ttcccctggg agtcaacccc
     1021 aatcacgccc gaagttgtcg catcaagctg ggctttgagc agggcgttgg tttcctgcaa
     1081 aatctggtcg gtgcgtttgc gttcggtcac atcgcgtgaa atcacgacca tgccgattac
     1141 ctgccggtct tcactgagca ggcggctgcc acgagtctcg acatagcgcc aggcgccgtc
     1201 gcgtcgggag aggcgaaatt caataacctg gtctgaaata ttgcgctgga cggcattccc
     1261 aaaggcttcc tgaacgccag gcaggtcgtc ggcatggata aactcaaaaa tgctgcgacc
     1321 tgtcacttct tcgggtgagt acccgagcat ccacttaatg gccgggcttt catagcggat
     1381 cacacccgtg gcgtcgagga tcgtgatcag atcaagtgta ttttcaacca ctgagcggaa
     1441 ccattcttcg tgttcctggg aatcacgcgg gtccaaccgt tttgagatcc tggcctgaac
     1501 ggctgcctga cgcaggcgga gctgatccat caccagggtg gccagcgtgg tcagaaatgc
     1561 ctgatctgcc tcagagactt cgcgcgggga ggtgtctgcc acggccagca ccccaatccg
     1621 gtgtccttcc ggcgtgatga tcggtgctgc caggtaaaag cggacgcctg ggggatggag
     1681 caccagtgga tgctcggcgg cgtgagagga cgtgaaggta tcgctgacag aataggtcgg
     1741 agatgtggtc tgcagtgctg agacgcacaa cccaggttcc agcggaagat gagttgtgtt
     1801 gagtccggta tgggctttga cccagacccg tgtctcatca ataaacccaa gccaggcaat
     1861 cggtacacaa aagtgctggg ccgccactgc caccacccga tcaaacaccg cttcagccgg
     1921 cgtgtcgagg atgtgcaggc gatggagttc agcaagtcgg tttgattcag ccaaaaggtg
     1981 ggggatcaac accattgtat actctgagta aaaggggttt agtgtgcggc ggcttgacgt
     2041 cgcgttgaga atagaaccgc gacgtcaagc tgcccgctcc aacgcttatt gctgtggtgc
     2101 cgggttcaat t
//
LOCUS       DAOQDO010001150         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50488_length_2111_cov_3.862840, whole genome shotgun sequence.
ACCESSION   DAOQDO010001150 DAOQDO010000000
VERSION     DAOQDO010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50488_length_2111_cov_3.862840"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PKZ53_16765"
     CDS             <1..1173
                     /locus_tag="PKZ53_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ42143.1"
                     /translation="HCSKHVHNLSAMTRREAEELVLKSNGNLCIRYLRQPEDQQIVTL
                     PGWMDRPALRRIALPIAAGVMAVALNSAPSVAQPPHRSKQVHSRPEKQPDRKPAKQGD
                     HSGSAILKGTVQYGQKEAVQDAEVTLINLETENKQIAISDENGHYQFIHIAEGIYKIE
                     ISAEGFDRVFVNEFMIRSGQQLQFDPQLEVGGATMGILTFSSSLEEMVKHRESRIECV
                     FDNPNSQAFFEYLEEGDQKEVLRMLKQGISPNLRNGCGETALMVACEDKGLISLLLQY
                     GADVKAQSMFGTTPLMFSMVQDDPAIPKVLIQHGAEVNAVDQDGRTALMIAAAAGKTR
                     FLKILVTAGADLNTKDKIGQTALNYAIAYDEEKAIAFLKLIGAKASPPIVEADEDE"
     gene            1204..>2111
                     /locus_tag="PKZ53_16770"
     CDS             1204..>2111
                     /locus_tag="PKZ53_16770"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ42144.1"
                     /translation="MRQARNILSQIQVKSPCTEDWNEMVGNDEVRFCSHCSKHVHNLS
                     AMTRREAEELVLKSNGNLCIRYLRRPEDQQIVTLPGWMDRPALRRIALPIAAGVMAVA
                     LNTPPSMAQNSPSAKPAIQVSDPARGKSSFQAAGLKSELSLEGVVKDTTGAVLAGASV
                     KCTNPLTGESLTTTTNEHGVFRIGILLEGDFVLDVEAAGSMADQLPRIRVVKGENQKV
                     EVVLQPSGPGEIIGNGTGEVLITHSDGSITPTSDQELSLEHCSNSKPSQSNSSQDKNS
                     PKVNSQSVQGVKPASPITKLSCQGIYG"
BASE COUNT          588 a          484 c          541 g          498 t
ORIGIN      
        1 cattgcagca agcacgtcca caacctgtcc gccatgaccc gccgcgaagc tgaagaactt
       61 gtgttgaaat caaatgggaa tttgtgtatc cggtatcttc ggcaaccaga agaccagcag
      121 attgtgacac ttccaggctg gatggaccgt ccggcgctga ggcggattgc gttgccgatt
      181 gccgctgggg tgatggctgt agcgctcaat tcagcgcctt cggtggcaca accaccacac
      241 cgatcaaaac aagtgcattc cagaccagaa aagcaacctg atcgaaagcc agcaaagcaa
      301 ggagatcact ctggtagcgc gatcttgaaa ggtacagttc aatatgggca aaaagaagct
      361 gtccaggatg cagaagtaac attgatcaac ctggaaactg aaaacaaaca gatcgcgatt
      421 tctgacgaga atggacatta tcaattcatt catattgctg aagggatcta caaaattgaa
      481 atttcagcgg agggctttga ccgagttttc gtgaatgaat ttatgatccg aagtggtcag
      541 cagctccagt ttgatccgca attagaagtt ggtggtgcaa ccatggggat actcactttt
      601 tcgtcttcgc ttgaagagat ggtcaaacat cgtgaatccc gtattgagtg cgtttttgac
      661 aacccgaatt cacaggcatt ttttgaatat cttgaagaag gggatcagaa agaagttctt
      721 cgaatgttaa aacagggaat ttccccaaat ctccgtaacg gctgcggcga aaccgcgctc
      781 atggttgcct gtgaggataa aggtttgatt tcgttgcttt tacaatatgg cgccgatgtg
      841 aaggcacaga gtatgtttgg gacgactccg ttgatgtttt ctatggtgca agatgacccg
      901 gcgattccaa aggtattgat tcaacatggt gccgaggtaa atgctgttga ccaggatgga
      961 cgaactgcct tgatgattgc ggcggcggct ggaaaaacga gatttctgaa gattctggta
     1021 actgctggtg ctgacctcaa tacaaaagac aagattggtc aaacggcgct caattacgcc
     1081 attgcttatg atgaggaaaa ggcaattgct tttctcaagt tgatcggcgc caaagcttca
     1141 ccacctattg ttgaagcaga tgaagacgaa tagccctaca ccagaaaaaa tgaagggaag
     1201 tttatgaggc aagctcgcaa cattttaagc cagatacagg tgaaatcacc ctgcaccgaa
     1261 gattggaatg aaatggtcgg taacgacgaa gtgcgtttct gcagccactg cagcaagcac
     1321 gttcacaacc tgtccgccat gacccgccgc gaagctgaag aactcgtcct gaagtcaaac
     1381 gggaacctat gtatccgcta ccttcggcga ccggaagacc agcaaattgt gacacttcca
     1441 ggctggatgg accgtccggc gctgaggcgg attgcgttgc cgattgccgc tggggtgatg
     1501 gctgtagcgc tcaatacgcc gccctcaatg gcccaaaact caccttcggc aaaaccggct
     1561 atccaggtca gtgatccagc caggggaaaa tcttcctttc aagcggctgg actgaaatcc
     1621 gaactgtcct tagaaggagt cgtcaaagat acgactggtg ccgttctggc tggagcaagt
     1681 gtgaaatgca ccaatccatt gactggagaa tcgctaacga caaccaccaa tgaacatgga
     1741 gtctttcgga ttgggatttt gcttgaagga gactttgtcc ttgatgtcga agccgccggg
     1801 tccatggctg atcagttacc acgaatcagg gtggtcaaag gggagaatca aaaggtagaa
     1861 gttgtgcttc aaccctctgg tcctggtgag attattggca acgggactgg cgaagttctt
     1921 atcactcact cggatggttc aataactccg acttctgacc aggagttatc tttggagcac
     1981 tgttccaatt caaaacccag ccaatcgaat tccagtcagg ataaaaattc accaaaagtg
     2041 aatagccagt ctgtccaggg agtcaaacct gcttctccaa ttacaaaact ttcttgccag
     2101 ggaatttatg g
//
LOCUS       DAOQDO010001151         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50501_length_2111_cov_2.842412, whole genome shotgun sequence.
ACCESSION   DAOQDO010001151 DAOQDO010000000
VERSION     DAOQDO010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50501_length_2111_cov_2.842412"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..733)
                     /locus_tag="PKZ53_16775"
     CDS             complement(50..733)
                     /locus_tag="PKZ53_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42145.1"
                     /translation="MSRILLITLFFLLSATSSVMAQQQFEITNASQYFDIKISVETCE
                     EGSCRGKASFSFFKKGGTTPYQVITLPDTYISLDEQGNPDVNISLLYDQQSVINIGDF
                     NFDGMEDVAICNGANGSYGMPSYSVYLSNRAARKFVYDKRFSLLGTHLGMFEVNSDQK
                     TLRTFDKSGCCYHVTEEFTVVNNAPQKVLEIIEDATIEDESKVKITTKRLVNGKWKTE
                     VKYETRDEE"
     gene            complement(881..>2111)
                     /locus_tag="PKZ53_16780"
     CDS             complement(881..>2111)
                     /locus_tag="PKZ53_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42146.1"
                     /translation="LRAKDEGDHDTKNQPDPPKNDKGESDPTWDRPDMMKLTKLDKDG
                     KELWTKDLAKKGGNAFGFISPMSPNRKAEEKGRIAGHDSHSSTSRIGFTTYKGTPIIF
                     AFYGGATEWDKAISGRHQNAYWRTLNAKTGEPVENLNGSAMAHSFDSQLLVSDEGIIT
                     AERSDSGLLMANYLYTRSNPLIFHFFYATTTNGNECFTQLGSLTPADDGYLILIAGNN
                     SESIIGKSVSGKASQAEALRNRNLMVIRIKKGFAQEIDALIDKPESKQVLDDILNSMK
                     SSPERDGLNLIGRTYLTHYGGDEPYSASRPKMVRLADGNYVVLWERWTHTLNAEKTGI
                     EGSFDSTWAMKINKDGEVLKEAVKLSDSVRITRGDEPVLWNGKASFLAGDVVDGKMLI
                     HQVDGQLNYSVLTVPLN"
BASE COUNT          507 a          541 c          458 g          605 t
ORIGIN      
        1 tcattcttca ttcttcattc tcaattcttc attcttcatt cttcattctt cattcctcgt
       61 ctcgggtttc atatttgact tcggttttcc atttcccatt caccagtcgt ttggtggtga
      121 ttttgacctt tgattcatct tcaatggttg cgtcttcaat gatttcaagg accttttgtg
      181 gtgcattgtt gacgacggtg aactcttcgg tgacgtggta gcaacacccg cttttgtcga
      241 aggtacggag ggttttctgg tcgctattaa cttcaaacat tcccagatga gtgcccagca
      301 ggctaaacct tttatcgtag acaaatttcc gggcagcccg attggacaga taaaccgaat
      361 acgacggcat gccataggag ccgttggcgc cgttgcaaat cgcgacatct tccatcccat
      421 caaaattaaa gtcgccgatg ttgatcacgc tttgctgatc atagagcaat gaaatattca
      481 catccggatt gccctgttca tccagcgaaa tgtaggtatc aggcaacgtg atgacctgat
      541 agggtgtggt gccccctttt ttgaaaaatg aaaaggacgc tttcccacgg cagctccctt
      601 cctcacaagt ttcgacactg attttgatgt caaaatattg agacgcattg gtaatctcaa
      661 attgttgctg cgccataacc gaagacgtgg ctgaaagaag aaaaaacaga gtgataagca
      721 agattcgact catggtttct gggtactcct caaaataaag gtgctgaagt tcaagcttca
      781 ccaggcactt gaatagtgcc tcgtaacaac ttttgcaagg caaagcttga acccccaacc
      841 aaactcactt tctaaaaggc ttgtgctgga cgttctctca tcaattgagc ggcaccgtca
      901 acactgagta gtttaactgg ccatctacct gatgaatcag cattttaccg tcaaccacat
      961 cgccagctaa aaacgaagcc ttgccattcc acaacaccgg ctcatcgccc cgcgtgatgc
     1021 ggactgaatc agagagtttg accgcttctt tcaacacttc gccatctttg ttgattttca
     1081 tggcccaggt tgaatcaaag ctgccttcaa taccagtctt ctcggcattg agggtgtgcg
     1141 tccagcgttc ccacaacaca acatagtttc catcagccag ccgaaccatt tttggacgcg
     1201 aggcactgta tggttcatcc ccgccataat gcgtgagata ggttcgccca atcaggttca
     1261 acccatcgcg ttccggcgaa gatttcatgc tgttcaaaat atcgtccaga acctgtttgc
     1321 tctccggttt atcaatcagg gcgtcaattt cctgggcaaa tcctttttta atccgaatca
     1381 ccatcagatt ccggttgcgc aacgcttcag cctgcgatgc ttttccgctg acactcttgc
     1441 caataatgga ttcactgtta tttccagcaa tcagaatcaa atacccgtca tcagccggcg
     1501 tcagcgaacc caactgggta aaacattcat taccattggt ggtggtggca taaaagaaat
     1561 ggaaaatcag cggatttgac cgtgtgtaca gataattggc catcagcagg ccgctgtcgc
     1621 tgcgctcagc ggtgatgatt ccttcatccg aaaccagcaa ctgactgtca aaactgtggg
     1681 ccatcgccga gccattcagg ttttcaaccg gttcaccggt tttggcattc agtgtccgcc
     1741 agtaggcatt ctggtgccga ccactgatgg ctttatccca ctcagttgca ccaccataga
     1801 aagcaaaaat aatcggcgtt cccttatagg tggtaaaccc aatgcggctg gttgaagaat
     1861 gcgaatcatg gccggcgatt cggccttttt cttcggcttt gcgattgggt gacatcggcg
     1921 aaataaaccc aaacgcattt ccgccttttt tggctaaatc tttggtccac agctcttttc
     1981 cgtctttatc gagtttggtc aacttcatca tatccgggcg atcccaggtt ggatcagatt
     2041 cacctttgtc attttttggt ggatcaggct ggtttttggt gtcgtggtcg ccttcatctt
     2101 tggctctgag a
//
LOCUS       DAOQDO010001152         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50506_length_2111_cov_2.684825, whole genome shotgun sequence.
ACCESSION   DAOQDO010001152 DAOQDO010000000
VERSION     DAOQDO010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50506_length_2111_cov_2.684825"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..2093
                     /locus_tag="PKZ53_16785"
     CDS             327..2093
                     /locus_tag="PKZ53_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42147.1"
                     /translation="MNWQLKRLGNGVWAMALLGNIKSGTLPNLLRALSLRRATATLVC
                     EQTPQIRATVHMIDGKLRRVTLGDLYGEPALSQLFRWKEGYYYLIKGRIEENVGPISA
                     PTGANDPRKHILVVDDDAQATALIERYLEREGYRVSIAFEGKHALELIANVKPDLVLL
                     DVMMPGMNGLEVARRLREKPETENLPIIIISAVVREMPEAYRQWDWPFVSKPFKAQTL
                     LQTVKDELLRQPLPPPEFTVSRLDFDPSISPEIIDLLIAELPLPPPDTVFVKSEALEI
                     RLALGSFSEETVRLFDLFDGTRTLGEIVNEYSLVASKINFLAVHMATFGLLEKLDGPV
                     CPQPTGKTTTLLLSQLDAIDQEQKPPITGSLVSTDAEHPEPEEVPTLLLTPTEIGPLS
                     ARLKDRYGPSTMCAKLAVFGLVGRVQSVFTSTLRLLARKFQNELTEPVKYHLPPLPGT
                     EVARLPLATDCGLFVYQLSVDSEYPKLVRQVGNDMWGILLLTDVQNRFEVNYTRYLIQ
                     AVAEPYQLPYLVALFDPRKKGINIAPIAREMEISTDYIATLDVSSPNSFLDVLRKLIQ
                     QPFPQPEKKNDLNEELRMKNEE"
BASE COUNT          552 a          565 c          471 g          523 t
ORIGIN      
        1 caccaagaac caagcaccaa gaaccaagca ccaagaacca agcaccaaga accaaacacc
       61 aggtattttc ttcattctca attcttcatt ctcaattctt cattcttcat tcctcattct
      121 tcattcttca ttcccgttga tctcgctgga atttgactgg aaaaaagtac gaaaaatcag
      181 tactgtttaa acaccacgtc tctccacaat aaacactcag ccaggtcaac cagtcacctc
      241 ttcccccaac cgaggtgcat ttctttttcc aaagttattg ttttgcttgt ttttctgatg
      301 gagcagggtt tttgagcctg gattcattga attggcaact gaaacggctt ggaaatggtg
      361 tctgggctat ggcactcctt ggcaatatca aatccggtac ccttcctaat ttgctgcggg
      421 cgttgagcct tcgacgtgcc acagccacac tggtctgtga acaaacaccg cagatccgtg
      481 ccactgtgca tatgatcgat gggaagctcc gccgggtcac actgggtgat ttgtacggag
      541 aaccagccct ctcacagttg tttcgctgga aagaaggcta ctactacctg attaaaggcc
      601 ggattgaaga aaatgtcggc ccaatcagtg ccccaaccgg ggccaatgat ccacgcaagc
      661 acattctggt ggtggatgac gatgcccagg caaccgcgtt gatcgaacga tatctggagc
      721 gcgaaggcta tcgtgtcagc attgcctttg agggcaaaca cgccctggaa ctgattgcca
      781 atgtcaaacc tgacctggtt ttgctcgatg taatgatgcc ggggatgaat ggcctggaag
      841 ttgcccgccg tctgcgagaa aaacccgaaa ctgaaaacct gccgattatc attatcagtg
      901 ctgtggtacg ggaaatgccc gaagcctatc ggcagtggga ctggccgttt gtctcaaaac
      961 cctttaaagc ccaaaccctg ctccagaccg tcaaagatga actgctccga caaccactgc
     1021 caccacctga atttacggtt tcacgcctcg attttgaccc cagcatctcg cctgagatta
     1081 ttgacctgct cattgctgaa cttccgcttc ccccgccaga taccgtgttt gtcaaatctg
     1141 aagctctgga aatccggttg gccttggggt cattttctga agaaaccgtg cgcttatttg
     1201 atttgtttga cggcacccgc acacttgggg aaatcgtcaa tgagtattca ctggtggctt
     1261 ccaaaatcaa tttcctggcc gtacatatgg cgacctttgg gctgcttgaa aaactggatg
     1321 gtccagtttg tccacagcca accggaaaaa ccaccacgct gttgctctct caactggatg
     1381 ccattgacca ggaacagaaa ccgcccatca ccggatcact ggtttcaact gatgccgaac
     1441 accctgaacc agaagaagtt ccaactcttt tgctcacccc gaccgaaatt ggaccacttt
     1501 ccgcccggtt aaaagaccgg tatggcccat caacaatgtg tgccaagctg gctgtttttg
     1561 gactggtcgg tcgagttcaa tcggtcttta ccagcacact ccggctcctg gcccgaaaat
     1621 tccagaatga attgaccgaa cctgttaaat atcaccttcc cccgttacct ggaaccgaag
     1681 tggcccgctt gccgctggca actgattgcg gattgtttgt gtatcagctt tcggttgata
     1741 gcgaataccc caaactggtg cgtcaggtgg gcaatgacat gtggggaatt ttgctcttga
     1801 ccgacgtcca gaatcgcttt gaagtcaatt acacccgcta tctcattcag gcggtggcgg
     1861 aaccatatca attaccatac ctggtcgcgc tgtttgaccc ccgaaaaaaa ggaatcaaca
     1921 ttgccccaat tgccagggaa atggaaattt caaccgacta tattgcgacc ctggatgtat
     1981 cgtcgcccaa ttcattcctg gatgtactgc gcaaactcat ccagcagcca tttccacaac
     2041 cagaaaaaaa gaatgatttg aatgaagaat tgagaatgaa gaatgaagaa tgaagaatga
     2101 agaatgaaga a
//
LOCUS       DAOQDO010001153         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50534_length_2110_cov_4.090998, whole genome shotgun sequence.
ACCESSION   DAOQDO010001153 DAOQDO010000000
VERSION     DAOQDO010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50534_length_2110_cov_4.090998"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKZ53_16790"
     CDS             complement(<1..194)
                     /locus_tag="PKZ53_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNJ42148.1"
                     /translation="MAPVKAATVGGDKDIVDTAVAAGSFTTLAKALQAAGLIETLKGK
                     GPFTVFAPTDEAFAKLPPGTV"
     gene            940..1872
                     /locus_tag="PKZ53_16795"
     CDS             940..1872
                     /locus_tag="PKZ53_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ42149.1"
                     /translation="MKQPLNYPIRAVSKLTGLSIDTLRAWEKRYQAVAPLRDERGRMY
                     TESDLERLHLLRAAVDKGHAIGRVATLTNQQLRELLELTPMVPASELVSVPMRKTQRH
                     LDLDPFMEAVERYDYLWIDKELGRLAILLPARELMYQVVLPLMRQVGIEWHQGKLSIA
                     QEHMVSSILSKLLGSLMRLHLNTNPPAMLLFATPSGEQHEFGILSSAMLATGGGLGIL
                     YLGCDLPAEEIAEVARRSDIDAVVLGVVGTGGINPAINSILFLTKHLPPRTELWVGGT
                     TSEELIYAIQQTRAQWIPHFEAYETHLKRLGARF"
BASE COUNT          489 a          517 c          595 g          509 t
ORIGIN      
        1 actgttcctg gaggaagttt ggcaaaagct tcatccgttg gggcaaacac tgtgaatggg
       61 cccttgccct tcaaggtttc gatcaggcca gcggcctgca gggccttggc cagggtggta
      121 aagcttcctg ccgcgacagc cgtgtcaaca atatctttgt ctccaccaac ggttgcggcc
      181 ttcaccggtg ccattgccac ggtgactgca aaaacaaacg ccatcatcaa gacgaccagc
      241 tttgatttca atttcatgat caaattcctc gtaagtgtgg gcgtcctgga tgccgaggta
      301 gctgtgtccc gtaccgggac gtaagaacgt tgctgctgat gacgaggtgg acaatactga
      361 gacattgaat cgttgtcaag tggttttcct ggacaaattc cggacaataa tttgacaaat
      421 atcagaatgg ccgctaagat cgaaaatgcc ccttcctaat ttcagttctg aattgagtca
      481 tatctttgtc aacaaaccca gccctcaagg gccgagcttg agtcgtggaa ccacgaggcc
      541 attctcgcag atggtccact gtatttgccc tggctcaagc tccggttgac cagaccccct
      601 gggaaatcca gggaaacgat agggaagaat tgataggtac cagcggatgc cgcctcagtc
      661 tgctgctcta cggtgacgag cgacccagga acgggggaag ttcgggcatg cgtcacagca
      721 aaccttccca gtcaggtcga gaggaggacg gattcgaccg atggtcacag tcgggtcgat
      781 acgcgcaact ctccacaagg agatggttga gggtattttt ccctcaagaa cggcgagtgg
      841 agggagttcg ctccttccac tttcatttca acttcttaca cagaaggagc ggcgctttct
      901 ctggtcctca atggccaggt ttccgagccg aagatgatga tgaagcagcc attaaattac
      961 ccgattcgtg cggtctcaaa gctcactggt ctgagtattg acaccttacg ggcctgggag
     1021 aaacggtatc aggcagtagc tccgctccgt gatgagcgag ggcggatgta cactgaatca
     1081 gacctggagc ggcttcatct gttgcgggcc gccgtggaca aggggcatgc cattgggcgg
     1141 gtggcgacgc tgacaaatca gcagctccga gaattacttg agttgacccc gatggtcccg
     1201 gcgagcgagc tggtctccgt gccgatgcgc aaaacgcagc gccatctgga tttggatccg
     1261 tttatggaag ccgtcgagcg ttatgattat ctctggattg acaaagaact tggacgactg
     1321 gccattttgc ttccggcgcg tgagttgatg tatcaggtgg ttctgccatt gatgcgccag
     1381 gttggaattg aatggcatca gggcaagctg agtattgcgc aagagcacat ggtgtcgtcg
     1441 attttgagta aactgcttgg ctcactgatg cgtctgcatc tcaataccaa ccccccagcc
     1501 atgttgctgt ttgccacgcc cagtggtgag caacacgaat ttggcatcct gtcatcggcg
     1561 atgctcgcca ctgggggtgg gttaggcatt ttgtacctgg ggtgcgattt gccagccgaa
     1621 gaaattgcag aagtggcgcg ccggagtgat atcgacgcgg tggttctcgg ggttgtcggt
     1681 actggtggaa tcaacccggc aattaacagt atcttgtttc taacgaagca tttgccgccg
     1741 cgaacggaac tttgggttgg tggtaccacc agtgaagaac tcatttatgc cattcagcaa
     1801 acccgtgccc agtggattcc tcattttgag gcatatgaaa cccatctgaa gcggctcggg
     1861 gccaggtttt aaacgtgttg gtgacagggt aaccctcttt cgcttctctc cactttcaag
     1921 cctgaacaga aaacctgttc aggctttgtt tttttgagcc gagagttcaa aaaatggagg
     1981 caggtcaccg acgaaagccg cggtggtttt tgaactggtg aggcagggtt caccgggtga
     2041 aaaaaaccgg aaaagtaccc ggaagaaaaa atgaatgagt gttcattctc ttgatttcct
     2101 gtgatatgct
//
LOCUS       DAOQDO010001154         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50545_length_2110_cov_2.918735, whole genome shotgun sequence.
ACCESSION   DAOQDO010001154 DAOQDO010000000
VERSION     DAOQDO010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50545_length_2110_cov_2.918735"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PKZ53_16800"
     CDS             complement(<1..1031)
                     /locus_tag="PKZ53_16800"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ42150.1"
                     /translation="MLKKRVFPSERLTAKWAACFLATLATVCFLAIAPVMAQQSFGNV
                     RGIVRDETGASIGGAKVTITEKATNRTISVQSGEDGTFQFNNLLVGEYQLLIEAQSFK
                     TATLSGIRVNLNTTNDIPITMTVGESGETVEVSAGGTELVQSTTTTLSKSFNSKQMVD
                     LAQTGNGSNTSDGIYSLALLSANVTSSGGVGVGSGGSVGGQRSRNNNFITDGIDNNDK
                     SVTGPSVYISPEVVAEFSLLTNQYSAEFGRNTGGQFITTTKSGSNEFHGTAYWYSRNR
                     YLNALDTLQKNAGVIRETVPGKSRLPRYDENRNGGNIGGPIIKDRLFFFTSFEGMQIG
                     QASGAGGIAT"
     gene            1209..1331
                     /locus_tag="PKZ53_16805"
     CDS             1209..1331
                     /locus_tag="PKZ53_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42151.1"
                     /translation="MRRLDAVFGVRRLDAAFGVRRLDAAFGVRRLDAAFTFDFG"
     gene            1448..1795
                     /locus_tag="PKZ53_16810"
     CDS             1448..1795
                     /locus_tag="PKZ53_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42152.1"
                     /translation="MSFPIKVLNRLRDGMLTAAEIQASHPSRRAWMNITPLPYEPHRE
                     FRRYIENRSFQVVRFEVDRDGIDPNYWLPDAAVHDLETLELSNEAQLEAYVLRFVPAL
                     EDLTAPWNCEMPS"
BASE COUNT          506 a          541 c          542 g          521 t
ORIGIN      
        1 gtggcaatac ctcccgcacc agacgcctga ccaatttgca tgccttcaaa cgaggtgaag
       61 aaaaacagcc ggtctttaat aattggcccg ccgatgttgc cgccgttccg gttttcgtca
      121 tagcgcggaa gccgggattt tccaggcacc gtttcacgga tcacacccgc atttttctgc
      181 aaggtatcca gagcattcag ataccggttc cgtgagtacc agtaggcagt cccgtgaaat
      241 tcattcgagc cgcttttggt ggtggtgata aattgtccac cggtattgcg gccaaactcg
      301 gctgagtact gattggtcaa caaggaaaat tcagccacaa cctccggaga aatatacacc
      361 gaaggtcccg tcactgactt atcgttgttg tcaattccat cggtaatgaa gttgttgttt
      421 cgggatcgct ggcctccaac tgacccgccg gagccaacgc cgacaccgcc gctgctggtc
      481 acgttggcag acaggagcgc cagactataa atcccatcgc tggtgtttga tccgttcccg
      541 gtttgggcca gatcaaccat ctgcttggaa ttgaatgact tggacagcgt tgtggtcgtg
      601 gactgcacca gttcggtacc accggcggaa acttcgaccg tttcgccaga ttccccaacc
      661 gtcatggtga ttggaatatc attggtggtg tttaaattga ccctgattcc gctgagggtt
      721 gcggttttga agctttgcgc ttcaatcagg agctggtatt caccaaccag caagttattg
      781 aactgaaagg tcccatcttc accactttgg actgaaatgg tccgattcgt agccttttcg
      841 gtaatggtga cctttgcgcc accgatggaa gcgccggttt catcgcgaac gataccacgc
      901 acgtttccaa acgattgctg ggccatcacc ggagcgatag ccagaaaaca aaccgtggcc
      961 agcgttgcca aaaagcacgc cgcccacttt gctgtcagcc tttcgctcgg aaacactctt
     1021 tttttcaaca taggaattct ctcctcacct ggaatgctgt gtttccgttt tttgatttga
     1081 agcgggcgcc tggagaaaaa ctgcagaaac acaaagtcac ggtgcaggag agaatctaaa
     1141 gaggattgga gaggctgaaa gcgaacatgt cgtaagcggc accaaacgcg gggcgagtgg
     1201 gattgggagt gcggcggctt gacgccgttt ttggagtgcg gcggcttgac gccgcttttg
     1261 gagtgcggcg gcttgacgcc gcttttggag tgcggcggct tgacgccgct tttacatttg
     1321 acttcggttg aaccaatacg gtcttcacgg aactgtttac tttgctgccg aaagaagagc
     1381 agatgcgagt gcgtgcagta gagtaaaatc gcaacccttc atttcaattt ggtggttgag
     1441 gtcaggtatg tcatttccaa tcaaagtttt aaatcggtta cgagatggaa tgctcacagc
     1501 ggctgaaatt caagcgtcac atccatcaag acgagcctgg atgaacatta caccgcttcc
     1561 gtatgagcca caccgtgagt ttcgccgata catcgagaat cgctcatttc aagttgtgcg
     1621 atttgaggtt gatcgagatg gaattgaccc aaattactgg ctgcccgacg cggctgttca
     1681 tgatctggaa acccttgaat tatccaatga agcccagctt gaagcatatg tccttcgatt
     1741 tgtgccagct cttgaggatc ttacagctcc ctggaattgt gaaatgcctt cttaaaccgc
     1801 aatcagatat tctgtttttt atgagaaaga catctcatct tagaattcct ttcagcaatt
     1861 cagaactaaa ctggcacctg aaacttgctg ggttaatcgt ggctttgaag gttgaagaat
     1921 tattctttac caggaaaaaa tgcgagaaag ccccgacctc agccgcgaaa cgtacgagga
     1981 cctgcccgcc ggcatcggga aatacaaaac cgacaagatg cgcaccatgc cccggcgcgg
     2041 cgtcatttac acggtagccc cctcgccgct cgacagcaac ctgatctggg ccggcaccga
     2101 cgacggcctg
//
LOCUS       DAOQDO010001155         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50572_length_2110_cov_1.921168, whole genome shotgun sequence.
ACCESSION   DAOQDO010001155 DAOQDO010000000
VERSION     DAOQDO010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50572_length_2110_cov_1.921168"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2110)
                     /locus_tag="PKZ53_16815"
     CDS             complement(<1..>2110)
                     /locus_tag="PKZ53_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42153.1"
                     /translation="GYFIFAGVQLYRLGPLQLEIGWSAVEQPKGCWKVNHVDPQGPAA
                     GKLQAGDQLVAINQESRVATHGPGYLRWVQPFGGPYTIRVIQNGTEKDFSLTSQLADT
                     HQMWPLLLMQFVIGAVFFGVGMSIGILKPDEFTTRLGSVTLLVAAITFLKNTLSTKVV
                     FLQGGSWVFWAPIHNLIGFQTALAFHFYYRFPATVPRGKFWTTCQYFFYGSALCLSLL
                     WWWLNSGKFLNPLALADFNYRTLDFWPVALQLLFFVHSGCIVMIFAVVLRNYRLVQDP
                     DQRRRIQWVSYGTLVAFFPLLVTALINTVLSPAPLSQLWWLTLLANLATIAIPISTAY
                     AILKHKVFDISVVIRLGLQYLLAKNVLQIILALPVIGLLYSVATNPHRTIAEFLFQNW
                     IYSGLVILSALSLQFRTPLRHWIDRRFFRQAYDGEQILLGLIGEVKELTSPTDIAHRV
                     SRSVEAALHPKHIQVFYRAPNSTALTLGYSSGSDSKKLYIPDSARILSLMEGHSTARE
                     FPFPASTRLPAWEQAWLEDLQVTLLVPMTGPDGLLVGLMMLGAKKSEQPYTHNDQRLL
                     QTLAHHMASVYQIEWLKHKVDQEQTLNREVLSRLEQNQVNLLKECPTCGECYDFSDQL
                     CPKDGATLVSTVPVERTIDSQYRLEHRLGKGGMGAVYQATDLRLARSVAVKVMTTSAL
                     GGAAALRRFEREARAAARLNH"
BASE COUNT          544 a          536 c          566 g          464 t
ORIGIN      
        1 gtgattgaga cgggctgcgg cacgggcttc gcgttcaaac cggcgtagcg ccgcggcgcc
       61 tccaagagca ctagtggtca ttactttcac ggcaactgac cgggctaatc ttaaatccgt
      121 tgcctgatac acggcgccca ttccaccttt tccaagccgg tgttcgagcc ggtactggct
      181 gtcaatcgtg cgttcgaccg gcaccgtcga gaccaacgtc gcaccatctt tggggcaaag
      241 ctgatccgaa aaatcataac actcaccgca ggttgggcat tctttcaaca ggttgacctg
      301 attttgttcc aggcgggaaa gcacttcccg gttgagggtt tgttcctggt caactttgtg
      361 tttgagccat tcgatttgat agacgctcgc catatggtgc gccagggttt ggagcaatcg
      421 ctggtcgttg tgggtgtagg gttgctcaga tttctttgct ccaagcatca tcagcccgac
      481 cagcagtcca tccgggccag tcattggtac cagcaatgta acctgcaagt cttccagcca
      541 cgcctgttcc caggccggga gtcgagttga ggctggaaaa gggaattcac gcgccgtgga
      601 atgcccttcc atcagactca atatccgggc cgaatctggg atatacagtt tctttgaatc
      661 actgccagat gaatacccaa gcgtcagcgc agttgaattg ggcgcccgat agaaaacctg
      721 gatatgtttg ggatggagcg ctgcctcgac actccggctg acacgatgag cgatgtcggt
      781 tggcgaggtc aattctttga cttcgccaat taaccccagc aggatttgtt caccgtcata
      841 ggcttggcgg aaaaaacgcc gatcaatcca gtggcgtaac ggggtccgaa actgcaaact
      901 gagggctgac aatatgacca gcccggaata aatccagttt tgaaacaaaa attcagcaat
      961 cgtgcgatga ggattggtgg caacgctata gagcaacccg atgaccggca aagccagtat
     1021 tatttgaagg acatttttgg ccagcaaata ttggagaccc agccggatga caacactgat
     1081 atcaaaaact ttatgtttga gaatggcata ggcggttgag atcggaatag caatcgtggc
     1141 caggttggca agcaaagtaa gccaccacaa ctgactcagt ggagcggggg agagaacggt
     1201 attgatcagg gctgtgacca ggagtgggaa aaaggccacc agtgttccat agctcaccca
     1261 ttggattcgc cgtcgctggt ctgggtcctg aaccaggcga taattgcgca ggaccaccgc
     1321 gaagatcatc acaatgcatc cagaatgcac aaaaaacagg agttgcaagg ccactggcca
     1381 gaaatccagg gtacggtagt taaaatcagc cagtgccagt gggttcagaa atttaccgct
     1441 gttgagccac caccagagga gtgacaggca cagggctgaa ccataaaaaa aatactgaca
     1501 ggtcgtccaa aatttccctc gtgggactgt ggctggaaac cggtagtaaa aatgaaaggc
     1561 cagtgcagtt tggaatccaa tcaggttatg gatcggagcc caaaataccc agcttccccc
     1621 ttgcaggaaa accactttgg tggaaagtgt gtttttcaaa aaagtaattg ccgcaaccag
     1681 cagggtgaca ctgcccaacc gggttgtgaa ttcatcaggt ttgagaattc caatgctcat
     1741 tcccacacca aaaaacacag ccccaatcac aaactgcatc aacaggagtg gccacatctg
     1801 atgggtgtca gccagttgac tggtcaggga aaaatccttt tctgttccat tttggatcac
     1861 acgaatggtg tatgggccgc caaagggttg gacccatctg aggtatccgg gaccatgggt
     1921 tgcaactcgg gactcctgat tgatggccac caactggtcg ccagcctgga gtttcccggc
     1981 tgccgggcct tgcggatcaa cgtgattgac cttccagcaa cctttgggct gctcgacggc
     2041 tgaccagccg atttccagtt ggagtggtcc aagccgatac agttgaaccc ccgcaaaaat
     2101 gaaatacccg
//
LOCUS       DAOQDO010001156         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50592_length_2109_cov_2.843233, whole genome shotgun sequence.
ACCESSION   DAOQDO010001156 DAOQDO010000000
VERSION     DAOQDO010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50592_length_2109_cov_2.843233"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PKZ53_16820"
     CDS             <1..413
                     /locus_tag="PKZ53_16820"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ42154.1"
                     /translation="TPDGRPVNQPQLIYPDSRLDSGRNFLRQGQEYNLPGSCLDGTHP
                     DECWYTITPPVGNEVFTIVYSRELLLSLVDRQSGINQQQVLEELVQINTPFVRRTSGS
                     SNGRNFEQLFSIFITSTNPNKRAELIERFLIKHQ"
     gene            complement(718..>2109)
                     /locus_tag="PKZ53_16825"
     CDS             complement(718..>2109)
                     /locus_tag="PKZ53_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNJ42155.1"
                     /translation="GGVTVSQGGAENGADVATTTDEQEDAAALQVSLSDIPAGISVRV
                     VNTAGTVRAFAQAACSVAPNIYPVSLTVSDTGVPVAPPLTTSTTFNVTVTGNAGPTLG
                     SYSNLTLVQGGSATQSPSAPPSDRNNNISRVTVTPTSLPGGGTLTVDTVSGAVRVTTV
                     SATQPGTYPVRVVVADPCVEASAGFQLVVNKPQTRPTAPTGLTAGAASSVQIDLSWND
                     NSDNEAGFVIERRTTTGQFAILATTNADVVRFSNSGLTPGVEYGYRVKATNGVGESDY
                     TNVASATPPVPTNLGVSQFFPLAAKSGGKILINGFGFVPGATEVYFGGDLQIRSPQVT
                     VFSPTQAEVTVPDSAGGSGNVNGFITVRVPGQPDANTRAFAQNSSDPNNPASTFPEFV
                     VLGDINRDGIVDERDVNELQSVVLGQKQFDARQALAGDVFPLNLNGSRGDGKFSGIDF
                     TVLRAVVLGQVAF"
BASE COUNT          526 a          566 c          534 g          483 t
ORIGIN      
        1 aaacacctga tggtcgcccg gtcaatcaac ctcagttgat ttacccagac tcacgacttg
       61 atagtggaag gaattttttg cgtcagggtc aggaatacaa ccttccgggt tcctgcctgg
      121 atgggaccca tcctgacgaa tgctggtaca ccatcacgcc accagttgga aatgaagtat
      181 ttactatcgt ttacagccgt gagcttctgt tgagcctggt tgaccgccag agtggcatca
      241 accagcaaca ggtcctggag gaactggttc agatcaacac accctttgtg cgtcgaacca
      301 gtggcagctc gaatggaaga aattttgaac aactgttttc catctttatt acgtcaacga
      361 acccaaacaa gcgggctgag ttgattgaac gctttcttat aaagcatcag taatctcagt
      421 ttgtagtcag aggtcaatgc tcaggctttt cagggttgat ggcagctaga gactgcggga
      481 aatacatatt tttcaactct ttagcctctt gtgcatacaa gagcaggtca ttcaaaaaga
      541 agagcggcgg aagaaggttt ttgatcagtt tcgttcctgc gaagaataga cattatcggg
      601 gttcaccatt ttcccaaagt ggagcttcct cgccgtggca accacccgct cacacgggtg
      661 gtactgactt ggtgacatcc ttttttcgat ggcctggttt atctccgaga aatcaattta
      721 gaaagcaact tgccctaaaa caaccgcccg cagcaccgtg aaatcaattc cagagaattt
      781 cccatctcca cggctaccat tgaggttcaa ggggaaaaca tccccagcca gggcctgacg
      841 ggcatcaaat tgtttctgtc ctaacaccac agactgcaat tcattgacgt cacgctcgtc
      901 aacaattcca tcccgattga tgtcacctaa caccacaaat tcagggaagg tcgaggccgg
      961 gttgttgggg tcacttgaat tttgagcaaa ggctcgggtg ttggcatccg gctggccagg
     1021 aactcgaacg gtaataaacc cattcacatt gcctgagcca ccagccgaat cagggacggt
     1081 cacctcagcc tgagttggtg aaaagaccgt tacctggggt gaccggattt gcaaatcacc
     1141 accgaaatag acttccgtcg cacccggcac aaacccaaat ccatttatga gaatttttcc
     1201 tccggatttt gcagccagtg ggaaaaactg tgaaaccccc agattggttg ggaccggagg
     1261 tgtggcactg gccacattag tatagtctga ctcgccaacc ccattggtgg ctttgactcg
     1321 atacccatat tcaacgcccg gtgtcagacc gctattgcta aaccgaacaa catcagcgtt
     1381 ggttgtggcc aggatggcaa actggccagt ggtagtccgc cgctcaatga caaaccccgc
     1441 ttcgttgtcg ctgttgtcgt tccaggaaag atcaatctgg acagaagacg cggccccagc
     1501 cgtcaatcca gttggcgcgg ttggtcgagt ctgaggttta ttgaccacca gttggaaccc
     1561 ggcgctggct tcaacacagg gatcagccac caccacccga accggatagg ttccaggctg
     1621 ggttgctgaa actgtcgtga cacggaccgc accactgaca gtatcaaccg tcagggttcc
     1681 gcctcccggc aaagaagtcg gggtcacagt cactcgtgaa atattgttat ttcggtcaga
     1741 tggtggggca gatggcgact gtgtcgccga gcctccttga accagagtca ggttagagta
     1801 actaccgagg gtcggcccag cgttccccgt cacagtgaca ttgaaggtgg tcgaagtcgt
     1861 caacggtggc gcaactggaa cgccagtgtc actgaccgtc aatgaaactg gataaatgtt
     1921 tggggcaacc gagcaggcgg cctgcgcaaa cgcccgtaca gtaccagccg tgttgacaac
     1981 ccggactgaa attccagccg gaatatctga aagcgagacc tgcaaggcag cggcgtcttc
     2041 ctgctcatcg gtggttgtcg ctacatcagc cccattttca gcccctccct gggaaacagt
     2101 cacgcctcc
//
LOCUS       DAOQDO010001157         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50620_length_2108_cov_6.009255, whole genome shotgun sequence.
ACCESSION   DAOQDO010001157 DAOQDO010000000
VERSION     DAOQDO010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50620_length_2108_cov_6.009255"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..322)
                     /locus_tag="PKZ53_16830"
     CDS             complement(200..322)
                     /locus_tag="PKZ53_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42156.1"
                     /translation="MSYSKIELVQGNSGTPGLYLSRNKQELDVGKVVYILFEFL"
     gene            429..1115
                     /locus_tag="PKZ53_16835"
     CDS             429..1115
                     /locus_tag="PKZ53_16835"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNJ42157.1"
                     /translation="MSEFCTLIVSDIHLGSPVSRAEALSALLREHTFQRLILLGDVFE
                     NLKFDRLHPAHWELVRTIQALSKASSEVEVVWIEGNHDEGLCRRGMELLGQPVVEEFV
                     WEAGGTKFVALHGHQFDSLVAERPLLTEVGSHLNQALQKLDHRDLRLSRWAKRMSKKL
                     SGQSAKVAEKATAYGQEQRADYVFCGHTHLAMTAQAGSVRYINTGCWTDIPSTFVGIT
                     NQDVHLIPCT"
     gene            1236..>2108
                     /locus_tag="PKZ53_16840"
     CDS             1236..>2108
                     /locus_tag="PKZ53_16840"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HNJ42158.1"
                     /translation="MVSPSASLLRKAVHQHSLTSRQGVLERMFTAFFDGLVYNQIWED
                     PRVDLEALDLTPDSRVLTITSGGCNVLNYLVAQPRLIIGVDLNPYHMYLTRLKLTALE
                     HLPDYESFFEFFGCANQPENISRYDSFMAPNLDVHAREYWEGRTLTGRRRIDAFGTQF
                     YDHARNGYFLRFVAGLAHLLGKHPEKVLEAKTLAEQEQVFATEIAPFFDNVLVKGLAK
                     LPFLVFGLGIPPRQLEALCEDAQGNIIELYRSRVKKLTCGFPVAENYFAWQAIARRYD
                     CDQRQAIPDYLKAEH"
BASE COUNT          517 a          518 c          522 g          551 t
ORIGIN      
        1 tagccttctt ctttaatatc ctgaagggct ttggctacgc ggggagactt ggcaatgaca
       61 tcattgagca ggttgcccaa atcataggtc gtggtttgca actttttatc cgaatgctga
      121 tccatgattg tccccttggc ggtaggtagg ttggagaaaa gggaaatttg gaaagacgct
      181 gccaatccta aagaatgagc tataaaaatt caaacaaaat gtaaacaact tttccgacat
      241 ccagttcctg tttgtttcgt gagagataga ggccaggggt cccactgttg ccctggacca
      301 gttcaatttt tgagtaactc aaggttcata tttttctaac acgcacgtaa ttcacagctt
      361 acctgaccct ggtttagttg cacgcaccag cctccaactc tgttcctttt tcactttgac
      421 ctttgccaat gtctgaattt tgcaccttga ttgtctcaga catccatctc ggctcgccgg
      481 tgagccgggc tgaagcgctt tcggccttgc tcagggaaca cactttccag cggttgatct
      541 tgctgggcga tgtgtttgaa aacctgaaat ttgaccgatt gcaccccgca cactgggaac
      601 tggtccgcac aattcaggcc ttgtcaaagg catcttctga agttgaagtg gtctggatcg
      661 aaggcaatca cgatgagggt ctgtgccgtc ggggtatgga attgctcggt cagccggttg
      721 tggaggaatt tgtctgggaa gctggcggga cgaaatttgt agcactccat gggcatcaat
      781 ttgactccct ggtggccgaa cgccctttac tgaccgaagt tggcagtcat ctcaatcagg
      841 cactccagaa acttgaccat cgtgatctcc gcttgagtcg ttgggccaag cggatgtcga
      901 aaaagctctc tggtcaatca gccaaagtgg ctgaaaaagc aactgcgtat ggtcaggaac
      961 agagagctga ctacgttttt tgtggccaca ctcatctggc gatgaccgct caggccggtt
     1021 cagtacgata tatcaacacc gggtgctgga ccgatatccc ctccaccttt gttggaatta
     1081 caaaccagga cgtccatctg attccatgta cttagtacct ggtacctggt acttagtgct
     1141 tggtaatcaa tatcttccaa gaaccaagaa ccaagaacca agaaccaaga accaagaacc
     1201 aagaaccaag aaccaggaac caagaaccaa aacctatggt ctcaccatct gccagtttgc
     1261 tccgcaaagc agttcatcaa cactcgctga cgagtcgtca gggtgtgctc gaacgaatgt
     1321 ttacagcttt ttttgatggg ctggtctata accagatttg ggaagacccc cgcgtagacc
     1381 tcgaagccct ggacctcacg cctgacagcc gggttctgac gattacttca ggcgggtgta
     1441 atgttctcaa ttatctggtt gcccaaccgc gactgatcat cggagttgac ctcaatccgt
     1501 atcatatgta tttgaccaga ttgaagttaa ctgcacttga gcatctgccc gattatgaat
     1561 cattctttga atttttcggc tgtgccaacc agcccgagaa tatttcccgg tatgattcat
     1621 ttatggctcc aaatctggat gtccatgctc gtgaatactg ggaaggtcgt actctaaccg
     1681 gaagacgacg gattgatgcg tttgggactc agttttatga ccatgctcga aacggctatt
     1741 ttctgcggtt tgtcgccgga ctggcacatc tgctcgggaa acatcctgaa aaggtgttgg
     1801 aggccaaaac actggccgaa caggagcagg tgtttgccac tgaaatcgcg ccatttttcg
     1861 acaatgtgct cgtcaaaggg ttggcgaaac tgccgtttct ggtgtttggg ttgggaattc
     1921 cgccccgaca gcttgaggca ctttgtgaag acgcccaggg aaatatcatc gaactctatc
     1981 ggtcacgcgt gaaaaagctg acctgtggct ttccagttgc agaaaactac tttgcctggc
     2041 aggcaattgc ccggcgctat gattgcgacc agcggcaggc aatcccggat tatttgaaag
     2101 ctgagcac
//
LOCUS       DAOQDO010001158         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50688_length_2107_cov_3.349415, whole genome shotgun sequence.
ACCESSION   DAOQDO010001158 DAOQDO010000000
VERSION     DAOQDO010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50688_length_2107_cov_3.349415"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKZ53_16845"
     CDS             complement(<1..505)
                     /locus_tag="PKZ53_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNJ42159.1"
                     /translation="MKHRRPPANPNSPKPSRQSPVSQQPALPIQPAITLARRVSFDAL
                     MFVEQGDFLDSDVLDGFLQKALREGTGNAGHREADRRLAQEITFGVLRWRGWLDHQIA
                     RLASRSLQHIEPAVRTALRMGLYQIAFLSRVPESAAVNESVNLIKLQTQLVHLGGFTN
                     AILREALR"
     gene            complement(536..619)
                     /locus_tag="PKZ53_16850"
     tRNA            complement(536..619)
                     /locus_tag="PKZ53_16850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Leu,seq:caa)
     gene            934..1938
                     /locus_tag="PKZ53_16855"
     CDS             934..1938
                     /locus_tag="PKZ53_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNJ42160.1"
                     /translation="MTTRINRQWRLATRPVGLIDDSVFTWTEEPVRELNEGEFLVHNL
                     YLSLDPTNRMWAQHDTYLPAVPIGDVMRGGTIGVVEASRNPNFPEGAHVQGLLGWQDY
                     AISKGPGVSILPVNPHIPLSAYMGLFGHIGMTAYFGLLDIGQPKEGETLVVSAAAGAV
                     GSIVGQIGKIKGCHVVGIAGNDDKCDWIVKDLGFDSAINYKKENVAKALKEHCPKGID
                     IDFENVGGEILDAILAQINLRARIVLCGLISQYNSKTPQPGPYNFANVLVQRARIEGF
                     IVLDYAARANEAFAELGQWMKEGKLKYRVDEVEGLEHAPQALNKLFEGSNIGKLVVKM
                     "
BASE COUNT          512 a          514 c          617 g          464 t
ORIGIN      
        1 ggcggagtgc ctcgcgcaaa attgcgttgg taaacccacc taaatgaaca agttgtgtct
       61 ggagtttgat gagattgacg gactcgttaa cagcggctga ctctggaacc cgagagagaa
      121 aggcaatttg atagagaccc atgcgaaggg ctgtgcggac ggctggttcg atgtgttgta
      181 gcgagcggct ggccagtcga gcaatttgat gatcaagcca cccccgccag cgtagcacgc
      241 caaaggtgat ttcctgggcc agtcggcggt cagcttcgcg atgcccggca tttccggtac
      301 cttcacggag tgctttctgc aaaaaaccat ccagcacgtc ggaatcgaga aagtcaccct
      361 gctcaacaaa catcaacgca tcaaagctga cacgacgggc aagggtgatt gcaggttgga
      421 ttgggagcgc tggctgctgg gaaacaggtg attggcgaga aggctttggg gaattggggt
      481 tcgctggtgg gcggcgatgt ttcactgacg aataaaagct gagggaaatg aaaaatgccg
      541 gaggagagac tcgaactctc acgaggttgc cctcaccgga ttttgagtcc ggcgcgtcta
      601 ccagttccac cactccggca tagaaaaaat ttgtgtgtga agagaacgcg catactagcc
      661 aaaaagcccc catccgtcaa cacccttttt tggtgaaaaa tcgagtcagc attcatgggt
      721 tgaaccgggt tggcgttctg gtggatctga tgtaaaaagc aggctctcgt ttctttcctg
      781 acatcaagtc agtaccactt tccgtgagcc ggttcaccca cccgctgaca caggtggtac
      841 tgaccatcgg tactactttc cgtgagccgg ttcacccacc cgctgacaca ggtggtactg
      901 accattcatc cttcaccaaa tggagcaaat caaatgacga cccgaatcaa ccggcaatgg
      961 cgactggcga cccggcccgt cggactgatt gacgatagcg tgtttacctg gaccgaagaa
     1021 ccagtgcgcg aattaaacga aggcgaattc ctggttcaca atttgtacct gtcgcttgac
     1081 ccaaccaacc gcatgtgggc ccagcacgat acctacttac cggcagttcc gattggcgac
     1141 gtgatgcgtg gcggcaccat cggtgtcgtt gaagcttcac gcaatcccaa tttcccggaa
     1201 ggtgcccacg tccagggcct gcttggctgg caggactatg caattagcaa aggacctggg
     1261 gtctcgattt tgccagtcaa cccgcatatt ccgctttcgg cttatatggg attgtttggg
     1321 catattggga tgactgccta ttttggactg ctcgacattg gccagccgaa ggaaggagaa
     1381 accctggtgg tttcggcggc ggcgggtgcc gttggctcga ttgtcggtca gattgggaaa
     1441 atcaaaggtt gtcacgtggt cggaattgcc ggcaacgatg acaaatgcga ctggattgtc
     1501 aaagatctcg gctttgactc ggccatcaat tacaagaagg aaaatgtcgc gaaagccttg
     1561 aaagagcact gtccaaaggg cattgacatt gattttgaaa atgtcggcgg cgaaatcctg
     1621 gatgccattc tggctcagat caatctccgg gcgcgcattg tcctgtgcgg gttgatttca
     1681 cagtacaact cgaagacgcc ccagccaggt ccatacaatt ttgccaatgt tctggtccaa
     1741 cgtgctcgaa ttgaaggctt tatcgtgctt gattacgctg cccgagccaa cgaagccttt
     1801 gctgaactgg gccagtggat gaaggaaggg aagctcaaat accgcgtgga tgaagtcgag
     1861 ggcctggaac atgcaccgca agccctgaat aagctctttg aaggttccaa catcggcaaa
     1921 ctggtggtga agatgtaatt gggctgaaga agacgggctt ggggctgaag actcgcaagc
     1981 tcggggctga agattttggg ctgaagaaga cgggcttggg gctgaagact cgcaagctcg
     2041 gggctgaaga ttttgggctg aagaagacgg gcttggggct gaagactcgc aagctcgggg
     2101 ctgaaga
//
LOCUS       DAOQDO010001159         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50695_length_2107_cov_2.844055, whole genome shotgun sequence.
ACCESSION   DAOQDO010001159 DAOQDO010000000
VERSION     DAOQDO010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50695_length_2107_cov_2.844055"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="PKZ53_16860"
     CDS             complement(<1..2094)
                     /locus_tag="PKZ53_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42161.1"
                     /translation="MLYYSKGDFKQAEVYSQQVLTIREKVFGPDHPDVAQILNNLGNL
                     YRSHGDYVQAELLLQRAQAIWEKVFGPDHPDVASSLNNLGLVYQAKGDYTQAELLLKR
                     ALAIREKKLGPNHPGIALCLNNLAVLSRIKGDDKQAEALLQRALVIWEQALGPDHQNV
                     LTNLNNLAVLYQAKGDIDQAIGYAVRTNEASERDLLRNLISGSERQKLLYLVQTKKFT
                     DQSISLNLQVAPQNPVARQTALTFLLRRKGRTLDALASAVETLRRQQKPETQKLLNDY
                     TSLVGQLSVLTLRGPGQMKLQEHLAILRLLEEQKEKMEAEISQQSREFQAQFTPITLT
                     EVQKQIPVDAVLVEFVLYPVYNAKTDTYTGPRYAVYTLDQTGKIMAADLGEASLIDQH
                     LANFHKVISTPTANLETEIKPASQILDQLIMKPVRALIGKARHLLISPDSNLSRLPFA
                     ALMDEKGKFLVENYRITHLTSGRDLLRLAVKIENHNPPLIMADPDYSTGKGPVLAGKQ
                     YVPLIRLVGTKLEGISLKNLFPDANLKMQAEATKQALKGVQRPEILHIATHGRFLEDK
                     PQPQLPQIAQRSLVPVEPQVNVEQLKLENPLLRSWLFFAGANHDGNDQNDGTLTALEA
                     AQLDLWGTKLVVLSACETGVGEAKTGDGVYGLRRALVLAGSESQMMSLWSVSDRATRE
                     LMVEYYTRLKAGEGRS"
BASE COUNT          447 a          514 c          586 g          560 t
ORIGIN      
        1 gctacggcct tcgccagctt tgagccgggt atagtattca accatcaatt cccgcgtggc
       61 ccgatctgaa acggaccaga ggctcatcat ttgggattcg ctgccggcca gaaccagcgc
      121 ccggcgaagg ccatacacgc catcaccggt tttggcttcg ccaacaccgg tttcacaagc
      181 cgagaggaca accagtttgg tgccccacag gtcaagttgg gcggcttcga gggcggtcaa
      241 cgtaccgtcg ttttgatcat ttccatcgtg gttggcgcca gcaaaaaaca gccaggagcg
      301 caggagtgga ttttccagct tgagttgttc aacatttacc tggggttcga ccggtaccaa
      361 tgagcgctgt gctatctgag gaagttgggg ttggggttta tcttccaaaa aacgcccatg
      421 ggtggcaatg tggaggattt caggtcgctg cacacctttg agtgcttgtt tcgttgcttc
      481 agcctgcatt tttagattgg catctggaaa gagatttttt aaggatattc cttcaagttt
      541 ggttccaacc aaccggatta atggaacata ttgtttgccg gccagcaccg ggcctttgcc
      601 ggttgagtaa tccgggtcgg ccataatcag tggtggattg tggttttcga ttttgacagc
      661 caatcgcaac agatcccgac cactggtcaa atgggtgatc cggtagtttt caaccaggaa
      721 tttccccttt tcatccatca gggccgcaaa tggaagccgg ctcaagttgc tgtctggtga
      781 aatcagcaga tgcctggctt tcccaatcag ggcacggaca ggcttcatga tcaattgatc
      841 cagaatctgg gacgcaggct tgatttcggt ttccagattg gctgtggggg tacttattac
      901 cttgtggaag ttggccaggt gctgatcaat caggcttgcc tctcccaaat cagcagccat
      961 gatttttccg gtctgatcga gtgtgtacac cgcataccgg gggccagtgt aagtatcagt
     1021 tttggcgttg tataccggat acaggacgaa ttccaccagg acggcatcca ccggaatctg
     1081 cttttgtacc tcagtcagcg taattggggt aaattgagct tgaaattccc ggctttgctg
     1141 actgatttcg gcttccatct tttccttttg ttcttcgagc aaccgcagaa tagccagatg
     1201 ttcttgtagt ttcatttggc cagggccgcg caatgtcagc accgagagtt gcccgacaag
     1261 gctggtatag tcattcagta gtttttgcgt ctcgggtttt tgttgacggc gcaaggtttc
     1321 aacggctgag gccagcgcat ccagggtgcg ccctttccga cgcaacaaaa aggtcaaagc
     1381 cgtttgtcgg gcaactggat tctggggagc tacctgcaag ttgagtgaaa tactttggtc
     1441 ggtaaatttc ttagtctgca ccagatagag caatttttgc cgctcagagc cggagatgag
     1501 gtttcgcagt aaatcccgtt cgctggcttc attggtacgc accgcgtagc cgatagcctg
     1561 atcaatatcg ccttttgcct ggtagagcac agccaggttg ttgaggttgg tcaggacatt
     1621 ttggtgatct gggccgagcg cctgttccca gatgaccagg gcccgctgaa ggagtgcttc
     1681 ggcttgtttg tcgtcaccct tgatacggga gagcacagcc aggttgttga ggcaaagggc
     1741 aatgcctggg tgattgggac cgagcttctt ctcacggatg gccagggccc gtttcaacag
     1801 gagctcagcc tgggtatagt cgcctttagc ttgatagacc agacccagat tgttaagact
     1861 ggaggcgaca tccgggtggt cagggccaaa cactttttcc caaatggctt gtgctcgttg
     1921 gaggagcagc tcggcctgta catagtcgcc atggctccta taaagattac ccaggttgtt
     1981 gaggatctgt gcgacatccg gatggtcagg accaaacact ttttcccgga tggtcaggac
     2041 ctgttgactg tacacttccg cctgtttgaa gtcacctttt gagtagtaca acatagccag
     2101 gttgttg
//
LOCUS       DAOQDO010001160         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50731_length_2106_cov_3.351536, whole genome shotgun sequence.
ACCESSION   DAOQDO010001160 DAOQDO010000000
VERSION     DAOQDO010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50731_length_2106_cov_3.351536"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKZ53_16865"
     CDS             <1..818
                     /locus_tag="PKZ53_16865"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ42162.1"
                     /translation="AVALIRLAVNLGMSIGPAVGGVLVLISFPSIFWIDGLTSILAGI
                     LLLVVWVKTRTPDRPHHHTTLVSQQSLSPAENRSILQDTHLVVFCVGAFLLGAVFFQH
                     EAAMPLFLVRDLKLAESTYGFMFTVNTLIIVFVELPLNSAMVNWSHRQSLMIGGILCA
                     LGFGSLAFVSGTLGVMATVVVWTFGEMIMFPAMVAYVADLAPAHRRGEYMGFYNMAFS
                     LALMVAPWAGTELLDRAGATILWFTCLAVGLLAAAIFSMASGSEMASGTEVAK"
     gene            complement(878..>2106)
                     /locus_tag="PKZ53_16870"
     CDS             complement(878..>2106)
                     /locus_tag="PKZ53_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42163.1"
                     /translation="RIAERTAELSETVMHLQSSEQKALEASQAKSVFLANMSHEIRTP
                     MNGVLGMASLLLKSGVTAEQKEYAETIKISAEALITVINDILDISKIEAGKLKLEVVD
                     FEFRPLLDDVNRLFQPAATRKNIELSLVIDPDIPTWLKGDPVRLRQILNNLVGNALKF
                     TEQGLVSLSVKVEKHLTADEWVLRFEVTDTGIGIPFDAQHQLFEKFYQADTSITRRYG
                     GTGLGLAISKQLVEMMGGDISLVSEVGRGTTFIFSACFLAGARPVVLVAGESPLHHRS
                     VRATSTIEKVLVVEDNRLNQKVIVSLLKKLGIEADIANNGQEALDAIKGGSYQLIFMD
                     CQMPVMDGFTATREIRRYETGDQHLPIIAVTANAMPEDELACYEAGMDGVITKPFTPE
                     KLKDVVDAWSQFSAKS"
BASE COUNT          460 a          547 c          530 g          569 t
ORIGIN      
        1 ccgctgtggc actcatccgg ctggccgtaa atctcggaat gagcatcggt ccggctgtgg
       61 gcggcgtgct ggtcttgatt tctttcccct ccattttctg gattgatggg ttgacctcta
      121 tcctggctgg aattctgctg ctggtcgtct gggttaaaac ccgaactcca gaccgcccac
      181 atcaccacac aaccctggtc agccagcaat cgctctcacc ggctgaaaac aggagcatcc
      241 tgcaagatac ccacctggtg gtgttttgtg tgggtgcgtt tttgctaggc gcggtgtttt
      301 ttcaacatga agctgccatg ccgttgtttc tggtccggga tttaaaactc gcggaatcaa
      361 cctatggctt catgtttact gtcaacacgt tgatcattgt ttttgttgag cttcccctca
      421 attcagccat ggtcaactgg tcacaccgcc agtcattgat gatcggaggc atcctgtgtg
      481 ctctcgggtt tgggtcactg gcttttgtca gtggcaccct gggggttatg gctacggttg
      541 tagtctggac ttttggagaa atgattatgt tcccagccat ggtcgcctat gttgctgacc
      601 tggcccctgc ccaccgacgc ggcgaatata tggggttcta taacatggcc ttcagcctgg
      661 ctttaatggt ggccccctgg gctggaaccg aactcctgga ccgggccgga gcaaccatcc
      721 tctggttcac ctgtctggcc gttggattac tggccgccgc tatcttttca atggcaagtg
      781 gaagtgaaat ggcaagtgga actgaagtag cgaagtagct cagctttgtg ttactgaggg
      841 gttgtgtcaa ctcctaaact ccctgtcatg cactctttca cgacttcgct gagaattgtg
      901 accaggcatc aaccacatct tttagttttt ctggtgtaaa gggtttggta atcacgccat
      961 ccattccggc ttcataacaa gccagttcat cttcgggcat ggcgttggcg gttacggcaa
     1021 taatcggaag gtgctggtca ccggtttcat aacgccgaat ttcacgggtg gcggtaaaac
     1081 catccatcac aggcatctgg cagtccataa aaatcaactg gtatgaacca cctttgatcg
     1141 cgtcgagggc ttcctggccg ttgttggcaa tatcggcttc gataccaagc tttttcagga
     1201 gactgacaat cactttttga ttcaggcgat tatcttcaac caccagcact ttctcaatcg
     1261 ttgaagtcgc cctgacggac cggtggtgga gtggggattc accagccacg agaacaacag
     1321 gccgggcacc cgccagaaaa caggcagaga aaataaacgt tgtcccacga ccgacttcac
     1381 tcacgagact gatgtcccca cccatcattt caaccagttg ttttgaaata gcgagcccaa
     1441 gcccggtgcc gccatagcgc cgggtgatgg acgtatcggc ctgataaaat ttttcaaaca
     1501 actggtgctg cgcgtcgaag ggaatcccaa ttccagtgtc ggtcacttca aaccgcaaga
     1561 cccactcatc agccgtgagg tgtttttcaa ctttgactga cagtgaaacc aggccctgtt
     1621 cggtaaattt taaggcgttt ccaaccagat tattgagaat ctggcgcaac cgaaccggat
     1681 cgcctttgag ccaggtggga atatccgggt caatgaccag gctcaactca atgttttttc
     1741 gggtggccgc tggctgaaat aaccgattga catcgtcaag gagcggtcga aattcaaaat
     1801 caacgacttc cagtttgagc tttccggctt cgattttgga aatatccaga atatcgttaa
     1861 tgacggtaat caacgcttcg gctgagattt tgatggtctc ggcatattct ttttgttcag
     1921 cggtcacccc gcttttgagc aatagcgatg ccataccgag cacaccattc atcggtgttc
     1981 ggatttcgtg gctcatattg gccagaaaca cactttttgc ctgactcgct tcaagtgctt
     2041 tttgctcaga ggattgcagg tgcatcactg tttcagaaag ttcagccgtc cgctcggcaa
     2101 ttcgtt
//
LOCUS       DAOQDO010001161         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50952_length_2101_cov_4.144184, whole genome shotgun sequence.
ACCESSION   DAOQDO010001161 DAOQDO010000000
VERSION     DAOQDO010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50952_length_2101_cov_4.144184"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKZ53_16875"
     CDS             <1..1359
                     /locus_tag="PKZ53_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42164.1"
                     /translation="LLKPALSALNQPQPDQINIAKPGTRNPKPETLLLKPALSALNQP
                     QPDQINIAKPGTRNPKPETLLLKPALSALNQPQPDQINTAKPGTRNPEPGTLLWADLG
                     EAETIDHAVTAFRQVLGNRTKNISTEVKRAAQNLDKLVFQPVRTLIGPARHLLISPDG
                     ALNLIPFAALVDAKGTFLVEKYTLTYLTSGRDLLRMQVKVPYQQPAMVIANPDYAVGQ
                     GPILAGRQIKPLVALPSTAKEGQEIQGLLEKAQLVIQTEATEAVVKKTKRPEILHIAT
                     HGYFLEDVPKAPSTSEPRILDFQDTGNLPPPRVENPLLRSWLFFAGANRGGDAENDGI
                     MTALEAAQLDLWGTKLVVLSACETGVGEAKNGDGIYGLRRALVLAGSESQVMSLWNVS
                     DRATRELMVEYYTRLKNGEGRSEALRKVQLKMLKDPQRQHPYYWAAFIQSGEWATLDG
                     KE"
     gene            1432..>2101
                     /locus_tag="PKZ53_16880"
     CDS             1432..>2101
                     /locus_tag="PKZ53_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42165.1"
                     /translation="MLAHLKTIIALSIINWALLSGLGFSSSSSALSLVFQAPNPEPRT
                     LNPAEKHTYPLTLKADDFLNLVVEQQGIDVVVRLVGPDGTVLIQVDSPNGTEGPEPLS
                     FIAELAGDYRLEVESLAKTGSPGKYQITLDPFRPATDQNRSRLEIKTLLAKIEALSLA
                     GNDTAALPLAIQALEKSEKTFGENEILTADSLQQLARIYHNQGAYAQAEPLYLRSLAI
                     REKML"
BASE COUNT          567 a          590 c          532 g          412 t
ORIGIN      
        1 cttctcaaac ctgctctatc agcgctcaat caaccgcaac cagatcaaat caacatagcg
       61 aaacccggaa cccggaaccc gaaacccgaa accctgcttc tcaaacctgc tctatcagcg
      121 ctcaatcaac cgcaaccaga tcaaatcaac atagcgaaac ccggaacccg gaacccgaaa
      181 cccgaaaccc tgcttctcaa acctgctcta tcagcgctca atcaaccgca accagatcaa
      241 atcaacacag cgaaacccgg aacccggaac ccggaacccg gaaccctgct ctgggccgac
      301 ctgggtgagg ccgaaactat tgatcacgca gtaacggcat ttcgccaggt cctgggaaac
      361 cgaaccaaaa atatttcgac tgaggtaaaa cgggcagctc aaaatctgga caaactggtg
      421 ttccagccag tgcgcacatt gattggccca gccagacatc tcttgatttc gcctgatgga
      481 gcgttaaacc tcatcccatt tgccgcgctg gttgatgcaa aagggacgtt tctggttgaa
      541 aagtacacat tgacgtatct gacaagtggg cgggacctgc tccgaatgca ggtcaaagta
      601 ccgtaccagc aaccagccat ggtcattgct aacccggatt atgcggttgg acaggggccg
      661 attctggctg gtcggcagat caagccgctg gtggcactcc ccagcacagc gaaagaaggt
      721 caggaaattc aaggtctgct ggaaaaggca caacttgtca tccagaccga agccactgag
      781 gccgttgtga aaaagacgaa gcgacccgaa atcctgcata tcgccaccca tggctacttt
      841 ctcgaagatg ttcccaaagc accttcgacc agtgaacccc ggatcctgga ctttcaggat
      901 acgggcaacc tcccaccacc gagagtggaa aatcccctcc tgcggtcatg gttgtttttt
      961 gccggtgcca atcgtggagg cgatgcagag aatgatggaa tcatgaccgc gctggaagcc
     1021 gctcagcttg atttgtgggg aaccaaactg gtggtgctct cagcctgtga gaccggggtc
     1081 ggagaagcca aaaacgggga tggtatttat ggtctgcgcc gggccctggt cctggctgga
     1141 agtgaatctc aggtcatgag cttgtggaat gtatcggacc gggcaacccg cgaactgatg
     1201 gttgaatatt atacccggct caaaaatggt gaaggccggt cagaggcgtt gcgcaaggtg
     1261 caactcaaaa tgctgaaaga cccgcagcgg caacatccat attattgggc ggcctttatt
     1321 cagtctggcg aatgggccac gctggatgga aaagaatgac cgattgacaa agtgacaaga
     1381 tgacaaaatg atggggtagc aagatgacac ggtggcagga aaatcgagaa tatgttggct
     1441 cacctcaaaa caatcatcgc actcagtatc ataaattggg ctctgctttc tggcctgggc
     1501 ttcagcagtt cttcttcagc cctcagcctt gtttttcaag ccccgaaccc tgaaccccga
     1561 accctcaacc cggctgaaaa gcacacctac ccgctcacac tcaaagccga cgattttctc
     1621 aatctggtgg tggaacaaca aggaattgat gtggtggtgc gccttgtcgg gccagatgga
     1681 accgttctga tccaggttga cagccccaat ggtaccgagg ggccagagcc actttcgttc
     1741 atcgcagaac tggcaggtga ttacaggctg gaagtcgaat cactggccaa aactggctca
     1801 ccgggaaaat accagatcac cctggatccg tttcgcccag ccacagacca gaaccgctca
     1861 cggcttgaaa tcaagacgct cctggcaaaa atcgaagccc tttctctggc cggtaacgac
     1921 acagcagcgc ttcccctggc tatccaggcg cttgagaaaa gtgaaaaaac ctttggcgaa
     1981 aacgaaattc tcaccgctga tagcctgcag caactggcac ggatctatca caaccagggg
     2041 gcttatgctc aggcagaacc gctctatctg aggtcgctgg ccattcggga aaagatgctc
     2101 g
//
LOCUS       DAOQDO010001162         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_50986_length_2101_cov_2.192571, whole genome shotgun sequence.
ACCESSION   DAOQDO010001162 DAOQDO010000000
VERSION     DAOQDO010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50986_length_2101_cov_2.192571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2101)
                     /locus_tag="PKZ53_16885"
     CDS             complement(<1..>2101)
                     /locus_tag="PKZ53_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNJ42166.1"
                     /translation="YHSVTEYVRTEMGKAQELGGSRKGSVGFALTALQRRLASSPEAI
                     FQSLKRRKERLEQRLREEKLNLRGQKYLAEILVDLPDDDDDLPAEEQECLEETLIDQA
                     TAARNIVELEGEITILRGLELQAKAVVASGQDRKWEELSRILQNDPAMRDAGGRLRKL
                     IVFSEHRDTLNYLHHRIAGVLGNAEAIVTIHGGTHRDERSRAQELFRADPEIRVLIAT
                     DAAGEGVNLQNANLMVNYDLPWNPNRLEQRFGRIHRIGQHEICHLWNLVARETREGDV
                     YHRLLKKLEVENEALKGRVFDILGEVFENISLKELLLQAIQYGDQPEKRAWLNQRIET
                     ALDHKHLQDILERNALAQESMNAERLFAVKEEMEKAEARRLQPFFVRSFFLNAFTTIG
                     GSIYPREAGRFEITHVPPFIRDRDRVLTGRNRRNSAPVLKRYERVCFTKEAVRPLDKP
                     GLPFATLLHPGHPLMLAVSDVLLEKHGNLLRQGAILVDPTDDSQDAHLLFLLTHEVKS
                     GDDQVLSKRLQFVNVAADGTATFAGWAPHLDLEPLLPDQRELLQELLNSPWIRADQEK
                     RAIALATTTLADEHFREVSSRRIAHVDKTLAAVHERLTKEISFWTDRLMKLKEDQAAG
                     KEVQPNPINIRRMINDLGGRLEDRKKKLLAMRGGEMSLIAPFRYTGLLFALALGWALW
                     GDVPNALAWVGIALIVGA"
BASE COUNT          453 a          556 c          582 g          510 t
ORIGIN      
        1 cgcccccacg atcagcgcga tgccgaccca cgccagcgcg ttgggcacat cgccccacag
       61 cgcccagccc agcgccagcg cgaacagcag gccggtgtag cggaaggggg caatcaggct
      121 catctcgccg ccgcgcatgg cgaggagttt cttcttccgg tcttccagac ggcctccgag
      181 atcattgatc attcggcgaa tgtttatcgg atttggttgg acttctttcc cggcggcctg
      241 atcctctttc agtttcatca agcgatcagt ccaaaaagag atttccttcg tcagacgttc
      301 gtggacggca gccagcgttt tgtcgacgtg ggcaatgcga cggctggaga cttcgcggaa
      361 atgctcatcg gccagcgtgg ttgtggccag ggcaatggca cgtttttcct gatcggcccg
      421 aatccagggc gagttgagaa gctcctgaag cagttcccgc tgatctggta agagtggctc
      481 caaatcaagg tgcggcgccc aaccagcaaa ggtggcggtc ccatcagcgg caacattgac
      541 aaactgaagg cgtttggaaa gtacctgatc gtctccggat ttgacttcgt gggtgagcag
      601 aaaaagcaga tgggcgtcct ggctgtcgtc ggttgggtct actagaatcg ccccctggcg
      661 aagcaggttt ccatgttttt ccaacaacac gtcgctgacg gccagcatca atgggtgtcc
      721 cggatgaagc aacgtggcaa acggaagccc aggtttatca agtggccgga cagcttcttt
      781 ggtaaaacaa acccgttcgt accgtttgag cacgggtgcc gaattgcgcc ggttacgtcc
      841 agtcaaaacc cggtcacggt cacgaataaa gggtggaacg tgcgtaattt caaagcgacc
      901 tgcttcgcgt ggatagattg acccgccgat ggtggtaaag gcattgagaa agaatgagcg
      961 aacaaaaaac ggctgaaggc ggcgggcttc ggctttttcc atttcttcct tcactgcaaa
     1021 aagccgttcc gcattcattg actcctgggc cagggcgttg cgttccagaa tatcctgaag
     1081 gtgtttatga tccagcgcgg tttcgatgcg ctggttgagc caggcacgtt tttcgggttg
     1141 atcgccatac tggatcgcct gtaacaacaa ttctttgaga ctgatgtttt caaagacttc
     1201 tccaagaatg tcaaagacac ggcccttgag agcttcgttt tcaacttcca attttttgag
     1261 caggcggtgg tacacatcgc cttcacgggt ttccctggcg acaagattcc agaggtggca
     1321 aatttcgtgt tgtccaatgc ggtgaatccg gccaaagcgt tgctcaagac ggtttggatt
     1381 ccagggaaga tcataattga ccatcaaatt ggcattttgg aggttcactc cttcgccggc
     1441 ggcatcagtt gcgatgagaa ctcggatttc cggatcggcg cgaaacagtt cttgagcccg
     1501 actccgttca tcccggtgtg ttccgccatg aatggtcaca atcgcctcgg cattccccaa
     1561 tactccggca atccggtggt gaaggtaatt gagcgtatct cgatgttcgg aaaagacaat
     1621 gagctttcgt aagcgtccgc cagcatcccg catggcaggg tcgttttgca gaatacggga
     1681 gagttcttcc cactttcggt cctggccaga agcaacaacg gctttggcct ggagttccag
     1741 ccctctgagg attgtgattt caccctccaa ttcgacgata tttcgggcgg cggttgcctg
     1801 atcaatcagg gtttcttcaa gacattcttg ttcctcagcc ggaagatcat cgtcatcatc
     1861 tggcaggtca accaggattt cagccaggta tttctggccg cgtaaattga gcttttcttc
     1921 gcggagccgc tgttcgagtc gttcttttcg gcgcttgagc gattgaaaaa tggcttctgg
     1981 actggaagca agtcggcgct ggagtgccgt cagggcaaaa cccacagatc ctttgcgtga
     2041 accacccagc tcctgggctt ttcccatttc ggttcggaca tattcggtta ccgaatgata
     2101 c
//
LOCUS       DAOQDO010001163         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51015_length_2100_cov_3.304156, whole genome shotgun sequence.
ACCESSION   DAOQDO010001163 DAOQDO010000000
VERSION     DAOQDO010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51015_length_2100_cov_3.304156"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..972)
                     /locus_tag="PKZ53_16890"
     CDS             complement(34..972)
                     /locus_tag="PKZ53_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42167.1"
                     /translation="MPALPALSAILITPDSFESIRKVVRHLTKQEVCNQIELILVAPS
                     RERLLLNPDDVTAFHSVQVVEVGVLDATPKGRAAGVRAAKAPVVVFVEDHVFPCHGWA
                     TALIEAHRQPWAAVGPTLKNANPQTITSWVNFLIEYSEWFNIPTARSVSHLPGHNCSY
                     KREVLLDYGDQLERFLDAESILHWDLHRRGFQLYLEPKAQILHLNFAVVGASIPLRFY
                     GGRLFAAARTKTMSPLHRGLYFILAPLIPLVRFLKICRFLKDSGNLSQFSISVFPAMA
                     MLLWVDGFGEMIGYGFGPGQANPILADLEFNRHRFM"
     gene            complement(978..>2100)
                     /locus_tag="PKZ53_16895"
     CDS             complement(978..>2100)
                     /locus_tag="PKZ53_16895"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ42168.1"
                     /translation="TRNPEPGTRNPEPGTRNPHMSSQSTINLGLIGCGTIAQLVHLPN
                     IARLPNVKLAALAESDAGQRAVVESRYPGIKSFADYRELLALAEIDAVLICLPTHLHA
                     QATIQALTQGKHVYLEKPLATSLAEGEQVLAAWKQSGLIGMIGYNYRFHPLYQKLKKL
                     LSLGKIGDLVQVRSVFSTSSHPLPAWRQSRQLGGGVLLDLASHHVDLVRYFFEQEIQQ
                     VSATVRSIRAEADTATLVMGLENGLTVESFFSQSAVDEDRFELYGRNGKLAVDRYQSF
                     DINFREARLDSHRLKQLKQSRQLIGRTPYILQKLLHPGFEPSYQHALSLFIQAIRRQT
                     SDFPDFSDGFTSLKVIIAAEASAASHQMVPVSEVALPSD"
BASE COUNT          552 a          526 c          559 g          463 t
ORIGIN      
        1 gacgactgac tactgatgga cgactgaccc atttcacata aagcgatgtc ggttaaactc
       61 cagatccgcc agaattgggt tggcctgtcc agggccaaat ccatagccaa tcatttcgcc
      121 aaacccatca acccacaata acatcgccat tgccgggaaa acgctgatcg aaaactgact
      181 gagatttcca gagtctttca gaaaccggca aatcttcaaa aagcgaacca gcggaatcaa
      241 gggcgccagg ataaagtaga ggccgcgatg aagtggcgac atggttttag tccgggcggc
      301 ggcaaacaga cgcccaccat aaaatctgag cggaattgaa gccccgacca ccgcaaaatt
      361 caagtgaaga atctgagctt tcggctcaag atagagttgg aacccacgtc gatgcaggtc
      421 ccagtgcaga atgctctccg catccagaaa ccgctcgagt tgatcgccat agtcaagcag
      481 cacttcgcgc ttgtatgaac aattgtggcc tggcagatgc gacacagatc tggcagttgg
      541 aatgttaaac cattcactat attcaatcag aaagttgacc caactggtga tcgtttgggg
      601 attggcattt tttaacgttg ggccaaccgc agcccagggt tgtcggtgag cttcaatcag
      661 tgccgtcgcc cagccgtgac agggaaacac atgatcttca acaaaaacaa caaccggagc
      721 tttggcggct cgaacccctg cagctcgacc ttttggcgtg gcatccagca caccaacctc
      781 aacaacttga acgctgtgga aggcagtcac atcatcaggg ttgaggagca accgctcgcg
      841 tgatggggca accagaatca gttcaatctg gttgcagacc tcctgtttgg tgaggtggcg
      901 aacaactttg cgaattgact caaacgaatc aggtgtaatc agaatggctg acaacgcagg
      961 aagagcaggc ataacgttca atcggaaggg agagcgactt ctgaaaccgg aaccatttga
     1021 tggcttgctg ccgaagcttc agccgcaatg ataaccttca aactggtaaa tccatctgag
     1081 aagtcgggaa aatccgaggt ttgacggcgg attgcttgaa taaaaagact cagtgcgtgc
     1141 tggtatgacg gttcaaatcc aggatgaagc agtttttgca gaatatacgg cgtgcgacca
     1201 atcagttgac ggctttgttt tagctgctta agtcggtgcg aatcaagccg ggcctcccga
     1261 aagttgatat caaacgattg ataccgatca actgccagtt ttccattgcg tccatacagt
     1321 tcaaagcgat cttcatccac ggcactttgc gaaaaaaacg attcaaccgt gaggccgttt
     1381 tccaaaccca tcaccagtgt cgccgtatcg gcttcggccc gaatggaccg gacagtggcg
     1441 gagacctgct gaatttcctg ctcaaagaaa taccgaacca ggtcaacgtg gtgtgatgcc
     1501 aaatcgagca acacgccgcc gccaagctgc cgggattgtc gccacgctgg caaggggtgg
     1561 cttgaggttg aaaagacgct tcggacctgg acgagatctc caatttttcc cagagaaagc
     1621 agctttttca gtttctgata gagtggatga aaccgatagt tgtatccaat cattccgatg
     1681 agacctgact gtttccaggc cgcgagcacc tgctctcctt ctgccaggct ggttgcgagc
     1741 ggtttttcaa ggtagacgtg tttgccttga gtgagggctt gaatcgttgc ctgggcgtgg
     1801 agatgcgttg gtaaacaaat caacaccgca tcaatttcag ccaaagccag gagttcacga
     1861 taatcggcaa acgactttat gccagggtag cgagattcga cgacagcacg ctgaccagcg
     1921 tctgattccg ccagggccgc cagtttcaca ttcggaaggc gggcaatatt cggtaaatga
     1981 accagttggg cgatagtgcc acacccgatg agcccaaggt tgatggtgga ttgagatgac
     2041 atatgtgggt tccgggttcc gggttccggg ttccgggttc cgggttccgg gttccgggtt
//
LOCUS       DAOQDO010001164         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51069_length_2099_cov_2.688356, whole genome shotgun sequence.
ACCESSION   DAOQDO010001164 DAOQDO010000000
VERSION     DAOQDO010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51069_length_2099_cov_2.688356"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PKZ53_16900"
     CDS             <1..1180
                     /locus_tag="PKZ53_16900"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNJ42169.1"
                     /translation="PISRPEISFFLPTQIIFGTGKVRHLAIDLHMAADLAEHTNVMLI
                     TDKGIVAAGLVDSLKHIFEHTDHEFKAIFDDVPPDSDVEVIKRAAQIATDNNINLIMA
                     LGGGSVMDTAKATSVVATYGGEPADYEGGFMVPGPIIPIIAIPTTVGTGSEVTLVAMV
                     KDHVNHRKILMSSPFLYPRMAVLDPEMVATLPAKIVAWTGMDALTHSIEAYTCSEHEP
                     FSEALALRSIEMIADNLVAAVAEAGPTEARAKMQLSATMAGMAFTNSPVGAVHAIAHS
                     IGALYGVHHGLANAIALPYVMEFNLEKATEGYAHVGRALGVGPDGSFREQAEAAIEIV
                     RTLKSQLGIPRLFREVGVPITDEALDQITNLTLEELCLAFNPRKASPEEIRDLIKQTI
                     "
     gene            1305..>2099
                     /locus_tag="PKZ53_16905"
     CDS             1305..>2099
                     /locus_tag="PKZ53_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ42170.1"
                     /translation="MSSPLKAESIPTKSLLIDGQLVAAGSGKTFQSLSPADQTPLANI
                     PHASLEDAETAVQAARSSFNLGSWRNSDGAMRAKILNKIADLIEANLEEFAQLEAADQ
                     GKPVGFARLLEIPAVVDCFRYFAGWTDRITGQTIPVPMSGLDFTLREPVGVCVGLAPN
                     NYPLALAAFKVAPALAAGNSFILKPSPSTPLTALRLGEICLEAGLPDGVLNVLTEPTN
                     EVAAYLLEHPEVDLVSLTGGTETGKAVMRGAASTLKHVSLELGGKSP"
BASE COUNT          468 a          555 c          554 g          522 t
ORIGIN      
        1 cccaatctcg cgtcctgaaa tttctttctt tctcccgaca caaatcattt tcggcactgg
       61 taaagtccgc catctggcca ttgatctgca catggccgct gatctggctg agcacaccaa
      121 tgtgatgctc atcactgata aaggcattgt ggctgccggg ttggttgatt cactcaaaca
      181 tatctttgaa cacacggatc acgagttcaa ggccatcttt gacgatgtcc cgcctgattc
      241 tgacgttgaa gtcatcaagc gagcggccca aatcgccact gacaacaata tcaatctcat
      301 catggcactg ggcggtggaa gtgtgatgga tacggccaaa gcgaccagtg ttgtggcgac
      361 ctatggtggt gaaccggcag attacgaagg tgggtttatg gtgccggggc cgattattcc
      421 gatcattgcc attcccacca cggtcggtac cggcagcgaa gtcaccctgg tggccatggt
      481 gaaagatcat gtcaatcatc gcaaaatcct gatgagcagc ccttttttgt atccacgaat
      541 ggcggttctg gatccagaaa tggtggccac gctgccagct aaaatcgtgg cctggaccgg
      601 gatggatgcg ttgacccact cgattgaagc ctatacctgc tctgaacacg aacctttttc
      661 agaagctctg gccttgcggt ctatcgaaat gattgctgac aaccttgtgg cggcagtggc
      721 tgaagccggt ccgaccgaag cccgtgccaa aatgcagttg tcggcgacga tggcgggaat
      781 ggcctttacc aattctcctg tgggggctgt tcacgcgatt gcccattcaa tcggggcgct
      841 gtatggggtt catcacgggc tggccaacgc gattgcgctt ccatatgtaa tggaattcaa
      901 tcttgaaaaa gccaccgaag ggtatgccca tgtcggtcgt gcccttggtg tcgggcccga
      961 tggaagcttt cgtgaacagg ccgaagcggc cattgagatt gtgcgaaccc tcaagtccca
     1021 gcttggaatt ccgcgtctgt ttcgtgaagt tggggttccg attactgacg aagcgctgga
     1081 tcagattacc aatttgacac ttgaggaact gtgcctcgct ttcaacccac gcaaggccag
     1141 tcccgaagaa atccgggatc tgatcaaaca gacgatctga acgaaatagt ggagttgtga
     1201 ggttgtgatt cttcgctgat ctgttgattt cgagacctgt gtttttcact tcttcgcgac
     1261 ttttctcaat tgctcccttg ctacaggcac aaaggacaca tcctatgtca tcaccactta
     1321 aagctgagtc gatcccaacc aaatcattat tgattgatgg tcagcttgtt gcggctggat
     1381 cgggaaaaac atttcaaagt ctctccccag ccgatcaaac gccactggcc aacattcccc
     1441 acgccagtct tgaggatgct gaaacggcag ttcaggcggc gcgctcatca tttaacctgg
     1501 gctcctggcg caattccgat ggcgccatgc gggcaaaaat tctgaataaa atcgctgatc
     1561 tgattgaagc caacctggag gaatttgccc agcttgaagc ggctgatcaa ggtaagccag
     1621 ttggctttgc acgcttactt gaaattccgg cggtggttga ttgtttccgg tattttgccg
     1681 gctggactga ccgcatcacc ggacagacca ttcccgtgcc gatgtctggc ctggacttta
     1741 cgctccgtga gccggttggc gtatgcgttg ggttagcacc aaacaactac ccactggcac
     1801 tggccgcctt taaagttgcc ccagccctgg cggctggaaa cagttttatt ttaaagcctt
     1861 ctccctcgac gccgttgacg gccttacgat tgggggaaat ttgccttgaa gcaggtctcc
     1921 cagatggtgt cctcaatgtt ttgactgaac ccaccaacga ggttgccgcc tatttactgg
     1981 aacaccccga agttgatctg gtctcattaa ctggtgggac tgaaaccggc aaggctgtga
     2041 tgcgtggggc ggcctcgacg ctcaaacacg tttcgctcga actggggggc aagtcgcca
//
LOCUS       DAOQDO010001165         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51116_length_2098_cov_3.166911, whole genome shotgun sequence.
ACCESSION   DAOQDO010001165 DAOQDO010000000
VERSION     DAOQDO010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51116_length_2098_cov_3.166911"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKZ53_16910"
     CDS             complement(<1..344)
                     /locus_tag="PKZ53_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ42171.1"
                     /translation="METRIDYSKFATSIYKAMLHLENQVSRSGIEPMLLHLIKLRASQ
                     INGCAFCIDMHSKDLRAGGESEQRLYLLNAWREAPFYTERERAALMLTEAVTLITNGH
                     VSDDVFMAVKPH"
     gene            686..>2098
                     /locus_tag="PKZ53_16915"
     CDS             686..>2098
                     /locus_tag="PKZ53_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNJ42172.1"
                     /translation="MAKKATFQDFSAKGKPEEQTLFRWVYDELRSAILEGRLRPGTRL
                     PSTRNLAQQFGVSRGTVVVVFEQLQAEGYIVSQTGSGTVVATALPDEFTEASQSPSSP
                     QSRLVSTRSLSERGACLLTNPFLIHRQTAVGKPFQSNTPAVEAFPIEVWSRLAGRRMR
                     RATRLLLSSGDPAGYRPLREALAAYLQTARGVRCEVEQILIVSGIQQALDLTTRLLLD
                     RGDPVWMEDPGYPGATSLFQGASLKLCPLPVDKSGIDTTVGTRDYPPARLVYLTPAHQ
                     FPLGVMLSLERRLALLKWAQSNGSWIFEDDYDSEYRYVERPLAALQGLDQHGCVLYAG
                     SFNKILFPALRIGYLVVPPSLVDAFTALRSTVDRFPAVMDQAILADFMSEGYFGQHIR
                     RTRELYHHRLQVFLESVNRRLGGWLEVAPTTAGLQTIGWLPQEADDQQIAQRAADFGV
                     ETIAMSRYSIQHRIRPGLILG"
BASE COUNT          474 a          529 c          537 g          558 t
ORIGIN      
        1 aagtggggtt ttaccgccat aaagacgtca tcggaaacgt gtccgttggt aatcagggtc
       61 acggcttccg tcagcatcaa agccgcccgt tcacgttcag tataaaatgg tgcttcacgc
      121 caggcattga gcaaatacag gcgttgctca ctttctcctc cggctcgtaa gtcctttgag
      181 tgcatatcaa tacagaaagc acacccgttg atctgtgagg cccgaagctt gatcaggtgc
      241 aacagcatgg gttcaatacc gcttcgtgaa acctgatttt cgaggtgcaa catggcctta
      301 tatatactgg tcgcaaattt tgaatagtct atccgtgttt ccatacaaaa tctccttttc
      361 gaatttaact gaagggaaat tcccgttgtg tgtgacacgg aaaacgtacc agaatatggt
      421 taagtcaaaa gatccattta gatcattttg attagaccat tttgtatatg gttcctgggt
      481 cttggttctt gggtcttggg tcttgggtct tggttcttgg aaggtacgta ccttgtcata
      541 agtttcctga gatattgggt ttggtaagtg gttgatttct tccgtggttt ccgtggttaa
      601 aatgtctgga aattttcggt aaagtactta ttgatttcca ctcaccagtt accaatctcc
      661 agtcaccaac ttccctcctt aacttatggc caaaaaagcg acttttcagg atttctcagc
      721 caaaggaaaa cccgaagaac aaaccttgtt tcggtgggta tatgacgaac ttcgcagcgc
      781 cattcttgaa ggccgactga gaccaggaac tcgccttccc tcaacccgca accttgcaca
      841 gcagtttggg gtttcgcgag gcacagtggt tgtcgtgttt gaacaactcc aggctgaagg
      901 atatatcgtg agccagaccg gatctggtac ggtggtcgca accgctttac cagatgagtt
      961 taccgaagcg tcccagtcac catcttcgcc tcaaagcaga ttggtttcga cgagatcact
     1021 ttcagagcgc ggggcttgct tgctgacaaa tccattcctc atccatcggc aaaccgctgt
     1081 tgggaaacct tttcaatcca atactccagc cgttgaagcc tttccaatcg aggtctggag
     1141 tcgtctcgca ggtcggcgga tgcgacgcgc aacccgcttg ctactgagtt ctggggatcc
     1201 agccgggtac cgtccgctcc gtgaagcgct ggcagcctac cttcagaccg ctcggggcgt
     1261 caggtgcgaa gttgaacaaa tcctgattgt ttcgggaatt cagcaggcgc ttgatctaac
     1321 gacccggctt ctgcttgatc gtggagatcc agtctggatg gaagatcctg gctaccctgg
     1381 tgccacaagc ctgtttcaag gtgccagcct caagctttgt cctttaccgg ttgataaaag
     1441 tggaattgac acaacagttg gaacacgtga ctacccaccg gcccgactgg tctatctcac
     1501 cccggcacat cagtttccgt tgggggtgat gttgtcgctt gaacgacggc tggccttgtt
     1561 gaaatgggcc cagtccaatg gaagctggat ttttgaagac gactatgaca gcgaataccg
     1621 ctacgttgaa cgcccgttgg cggcactgca gggcctcgac cagcatgggt gtgtgctcta
     1681 tgccgggagt tttaacaaaa ttctgttccc tgccctgcgc atcggatatc tggtggttcc
     1741 gccgtcactg gttgatgcgt tcacggccct tcgatcaacc gttgatcggt ttccagccgt
     1801 gatggatcag gccattctgg ctgactttat gagtgagggt tattttggcc agcacattcg
     1861 tcgaactcgg gaactctatc accaccgact tcaggtgttt ctggaatcag tcaaccgtcg
     1921 gcttggagga tggcttgagg tggcgccgac aactgccgga cttcaaacaa ttggctggct
     1981 tccacaagaa gcggatgatc agcaaatagc tcagcgggca gccgatttcg gagtggaaac
     2041 aattgcaatg tcacgataca gcatccagca tcggattcga cctggcttga tccttggc
//
LOCUS       DAOQDO010001166         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51160_length_2097_cov_3.078355, whole genome shotgun sequence.
ACCESSION   DAOQDO010001166 DAOQDO010000000
VERSION     DAOQDO010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51160_length_2097_cov_3.078355"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="PKZ53_16920"
     CDS             <1..1858
                     /locus_tag="PKZ53_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42173.1"
                     /translation="LTQPSQLVSDLPNAIDLVILKALAYDSKRRYQRARDFSDDLVQA
                     LELKTVVVQDPLDTIVLFGEHDTTLVPDAAPPTQKQILPEITDPGGGHLTRTGQLEDQ
                     NQRQRMIVGILSILLLATLIWNFVPTRINPVSTSQPPKVVTPTRSLAILPFRNLKPDP
                     ETDFLGFSLADAVITKLGYVQNLVVRPSSYVEKYRNQVIDPQQIARELNVDTLLTGSF
                     LCDGKDLRITVQLVDVTSNTSLWQDTIDVKYERLLTVQDLVAQQLISELRLQLTPGES
                     ERLASEIPRDPKAYEYFLKGVNLYVTNDYSTAARMFEKSIEIDPNYALAWAHLGRALN
                     GSASKLFGGREEYAKAIKAYEKALSINPHQIEARIFMANSFTDTNQVEKAVPLLREVM
                     ATNSNLAEAHWELGYAYRFAGLLPESIAECQKARKLDPNVKLTNSAFNSYLYAGQYDA
                     FISSLPQREDSAFILFYFGLGNYYQQRWSEAARYFDQAYEKDPNMLQTQVGKALSYAI
                     ANQPQPGLELIRKTARKVQERDVSDAEAVYKVAQAFAVLGDSPAALRLLRRTVEGGFF
                     CYPYFTSDPLLENIRQEPEFQAIMAIAQERHIAFKNRFLVAGSVLQKPTSTD"
     gene            1906..>2097
                     /locus_tag="PKZ53_16925"
     CDS             1906..>2097
                     /locus_tag="PKZ53_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42174.1"
                     /translation="MEKIFKNGFLKELFEVATAQDLNTSANQRNEKLMRAFLEFGSET
                     ECNLNCAAQIEYGLVTDQPY"
BASE COUNT          561 a          529 c          504 g          503 t
ORIGIN      
        1 cctcacccag ccgtcacaac tggtctctga tcttccaaat gcgattgatt tggtgatttt
       61 gaaagccctg gcctacgatt ccaaacggcg gtatcagcgt gcccgtgatt tcagcgatga
      121 tctggttcag gctctggaac tcaaaaccgt cgtggttcag gatccgcttg atacgattgt
      181 gctgtttggt gagcacgaca caacgcttgt tcctgacgcc gccccgccaa cccaaaaaca
      241 gatattgccc gagattactg acccaggagg tggtcatctg acccgaaccg ggcaactgga
      301 agaccaaaat caacgccagc gaatgatcgt cggaatcctt tcgatactac tcctggctac
      361 tctgatctgg aattttgtac caacccgaat caatccagtt tcgaccagcc agccaccgaa
      421 agtcgtgact ccaacccgga gtctggctat tttgccgttc cgcaatttaa aaccagatcc
      481 ggaaactgat tttcttgggt tttcgctcgc tgatgcggtg ataaccaaac tgggctatgt
      541 tcaaaatctg gtcgtcaggc cgtcgtccta tgtcgaaaaa tatcgcaatc aggtcattga
      601 tccgcagcaa atagctcgcg aactcaatgt tgatacactc ctcaccggca gctttctgtg
      661 tgatggaaaa gatttgcgga tcacggtgca actggttgat gtaacaagta ataccagcct
      721 gtggcaggac acgatagacg tcaagtatga gcgattgctc acggtccagg accttgtggc
      781 gcaacaactc atctcagaat tgaggttgca attgacacca ggggaatcag aacgactggc
      841 cagtgaaatt cctcgggatc caaaggccta tgaatacttc ctcaagggcg tcaatttgta
      901 cgtcaccaat gattattcaa ccgccgcccg gatgtttgaa aaatccattg aaattgaccc
      961 gaactacgcg cttgcctggg cccatcttgg gcgggcgtta aacgggagtg cttcgaaact
     1021 gtttggcggg cgcgaagaat atgccaaagc aatcaaagcc tatgaaaaag cgctgtcaat
     1081 caacccacac cagattgaag cccggatctt tatggccaat tcgtttaccg ataccaatca
     1141 ggttgaaaag gcggttccgc tcttgcgcga agtgatggcc accaattcaa atctggccga
     1201 ggcacattgg gaactggggt atgcctatcg gtttgcgggt ctcttgccgg agtcaattgc
     1261 cgaatgccag aaagctcgca aacttgaccc aaatgttaaa ttgaccaact ccgcattcaa
     1321 tagctatctg tatgctggtc aatatgatgc gtttataagc agcctgcctc agcgtgaaga
     1381 ttccgccttt attctgtttt attttgggtt aggcaactac tatcagcagc ggtggagcga
     1441 ggcagcccgg tactttgatc aggcctatga aaaagacccg aatatgctgc aaacccaggt
     1501 tgggaaagcc ttgagctacg cgattgccaa tcagccacaa ccagggctgg aattaattcg
     1561 caaaacagcc cgcaaagtgc aggaacgcga tgtcagtgac gctgaagccg tgtacaaagt
     1621 cgcccaggcg tttgcggtgt taggtgatag ccctgccgcc ctgcgcctat tgcgtcgaac
     1681 cgttgaggga ggttttttct gttaccccta cttcaccagt gatccgttgc ttgaaaacat
     1741 ccgccaggaa cctgaatttc aggcgattat ggcaattgcc caagaacgac atatcgcgtt
     1801 taaaaaccga ttcctggtgg caggatcggt tctccaaaaa cccacatcaa ctgattagaa
     1861 acgttgcccg aatgagtttt ggggaacctg gaggaaaaca acagggtgga aaaaatattt
     1921 aaaaatggat ttctcaaaga gctttttgaa gttgcgacag cacaagattt aaatacatcc
     1981 gccaatcaac gcaatgaaaa actcatgcgc gcgttcctcg aatttggatc tgaaacagaa
     2041 tgtaacctga actgtgccgc ccaaattgaa tatggcctgg ttacggatca accctat
//
LOCUS       DAOQDO010001167         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51199_length_2096_cov_3.266536, whole genome shotgun sequence.
ACCESSION   DAOQDO010001167 DAOQDO010000000
VERSION     DAOQDO010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51199_length_2096_cov_3.266536"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PKZ53_16930"
     CDS             complement(<1..1421)
                     /locus_tag="PKZ53_16930"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42175.1"
                     /translation="MSQLRTCVGIGLISLLLWTTTQPAFALGLGQDKNGPPPTTQTSE
                     QTAAEAQIKSLVDETNTLIQAGKLNDAIARATQALKLAEANFAPDHQLVYTSLDRLAF
                     AYFRKRNFNQALPLAKRALAMVEAKVPPNDPEIAMCLQPLIFIYKEQSQADQAEPLAQ
                     RAITILETAEGKEHPSLIGFLTMLGEIKLIQQKSLEARSVLERALAIAEKQAGPQSHR
                     LSPILFELSQTYQATRNYPKAEELYLRGVNLLEKGLGPQHPQVGFAYNNFVILLINQN
                     EFIRALPLQEKSVAILEKSLGPEHLEYASASHQLGQLYHKLSQFSQAQVVLNRALAIR
                     QKVYGPTHPLTVRTLTELSNVYLKTGNYTELEFIHRFLLDYLEKNQGMPAAEQLDLLI
                     GVAVGYHNQGNYAQTEALYFRILDLIQKHPELESEKLNQIQNNLAWVYAERRDFAKSE
                     PLFLEIIARNEKRYGRQSGELVL"
     gene            complement(1605..>2096)
                     /locus_tag="PKZ53_16935"
     CDS             complement(1605..>2096)
                     /locus_tag="PKZ53_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNJ42176.1"
                     /translation="VLTSGADKISMNSLAVRNPALVDESARQFGSQCIVVAIDAMRES
                     DGSYQVYTHGGRINTGRDAFEWAQEVVKRGCGEILLTCMQRDGTGLGFDTEFTGLLAR
                     TLPVQVIASGGARTPEDFYQAFVHDADAALAAGMFHRGEYRIREVKAYLAERGILVRG
                     TGL"
BASE COUNT          475 a          517 c          566 g          538 t
ORIGIN      
        1 aacaagacca gttctccaga ttggcggcca tagcgttttt cattgcgggc aatgatttcg
       61 agaaacaagg gctcggattt ggcaaaatcc cgccgctccg cataaaccca ggccagattg
      121 ttctgaatct gattcagttt ttctgattcc agttccgggt gtttttggat caggtcgagg
      181 attcgaaaat agagggcttc ggtttgggca taattgcctt gattgtggta gccaacggca
      241 acgccaatca gcaggtcaag ttgctcggca gcaggcattc cttgattttt ctcaaggtag
      301 tcgagcagga agcgatgaat gaattcgagt tcggtataat ttcccgtttt taaatataca
      361 tttgagagtt cggtcagtgt cctgaccgtc agtggatggg tcggaccata aactttctgg
      421 cgaattgcca gagcgcgatt taataccact tgagcctgag agaactggct gagtttgtga
      481 taaagctggc caagctggtg actggccgag gcatattcca gatgctcggg ccccagtgat
      541 ttttcaagaa tggcgactga tttttcctgt aacggcaagg ccctgataaa ttcgttctgg
      601 ttgattaaca ggatgacgaa attgttataa gcaaatccaa cctgagggtg ctgggggcca
      661 agtccttttt caagcaggtt gaccccgcgc aggtacagtt cttcggcttt cggatagttt
      721 ctggttgcct gataggtctg ggagagttca aacagaattg ggcttaaccg gtgggactgt
      781 gggccagcct gtttttccgc aatcgccagt gcccgttcca aaaccgaacg ggcttcgagc
      841 gatttttgct gaatgagttt gatctcgccc agcatcgtca gaaaaccgat cagggatgga
      901 tgctccttcc cctcagcggt ttcaaggatc gtgatggcac gctgcgccag cggttcagcc
      961 tgatcggcct gactttgctc cttatagata aagatcaacg gctggagaca catggcgatt
     1021 tccggatcat tcggagggac tttggcttcg accatggcca gggcccgttt ggccagcgga
     1081 agtgcctgat tgaaatttcg ttttcgaaaa taggcaaagg ccaggcgatc cagactggtg
     1141 taaaccagtt gatggtcagg tgcaaagttg gcttcagcca gtttgagggc ctgggtggct
     1201 cgggcgatgg cgtcattgag tttcccggcc tgaatcaacg tgttggtctc gtcaaccagg
     1261 cttttgatct gagcctcggc agccgtttgt tctgatgttt gagtggtcgg aggcggtcca
     1321 tttttgtctt gccccaatcc tagcgcgaag gcgggttggg ttgtcgtcca caggagcaag
     1381 ctgatcaatc cgatgccaac gcaggttcga agttgagaca tggtagctct ccccaaagtt
     1441 attcgaattt ttgatggtac tcgattggtt tgattgttaa atgaaaactc accacagagg
     1501 acacagaggc gcacagagga acatagacat tcggaatggt tgatgtttct ctgtgttcct
     1561 ctgtgtcctc tgtggttcaa aaatgatgtt gtttcatttc caatctaaag accagtcccc
     1621 ctgaccaaaa taccacgttc tgccagatat gccttcacct cacggatgcg atattcgccc
     1681 cgatgaaaca ttccagccgc cagtgccgca tcggcatcgt gaacaaatgc ctgatagaaa
     1741 tcttccggag tgcgggcgcc acccgaagca atcacctgca ccggcagtgt tcgcgccagc
     1801 aagcccgtaa attcagtgtc aaacccaaga ccggttccat cgcgctgcat acaggtcagc
     1861 aggatttcac cacagccccg tttgaccact tcttgagccc attcaaatgc atcacgcccg
     1921 gtgttgatgc gtcccccgtg ggtatagacc tgatagctgc cgtcagattc gcgcatggcg
     1981 tcaatcgcaa ccacaataca ctgtgaccca aactgacggg ccgattcgtc caccagcgcc
     2041 gggttgcgga cggcaaggct gttcatactg attttgtcgg cgccggaggt gagcac
//
LOCUS       DAOQDO010001168         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51314_length_2094_cov_2.614517, whole genome shotgun sequence.
ACCESSION   DAOQDO010001168 DAOQDO010000000
VERSION     DAOQDO010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51314_length_2094_cov_2.614517"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1242
                     /locus_tag="PKZ53_16940"
     CDS             157..1242
                     /locus_tag="PKZ53_16940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ42177.1"
                     /translation="MTNLSNSHLSQITSLLEKACHQGIIQGAQWMALKNGIVHGAGQA
                     GQAYEIYDLASLTKPLATALACLILKDQGKLDLDTPVSSYLSELDSPEKKSITVRQIL
                     IHTAGFAAWRPFYLLACHPDEIIATINQIPLEYEAGSKVVYSDLGYIVLGKMLEKITI
                     TPMEKLFQFLVAAPLNLKNLRYCPPKSWKPRIAPTEIGNQYEQGLAVRTIGQDWPTTH
                     QWRTTLIQGEVHDANAFFLNGGAGHAGLFGSIQDVAALSHQFLPGSQLLQPSTLKEFC
                     HNDTPGQNEARSPGWMLAQTPQCSAGNTCSPQTFGHVGFTGTSLWIDPESMTVTILLA
                     NRTFPIQDLTPLRRQFHSLATTLSNKD"
     gene            complement(1298..>2094)
                     /locus_tag="PKZ53_16945"
     CDS             complement(1298..>2094)
                     /locus_tag="PKZ53_16945"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNJ42178.1"
                     /translation="WLVHRGGQVLADGLGDSVFPPSVKIVSNQELAECSDLIVVLGGD
                     GTMITGARIVGKRPTCILGVNFGYLGYLTEFSPDNIFPALELVLAGKHQVDTRIKLEA
                     AVLRNGHEVSRVEVINDVVVNKALLARIIEIQCWIDGQFVSMFRADGLIISTPTGSTA
                     YSLSAGGPIIHPAMHAVVITPICPHTLTNRPLVVSDESVIELHLVATRGDIEDVTLTL
                     DGQIAISLFVDDRIVIRKSDSVLKLIQPPNRNYFQILRDKLKWGGE"
BASE COUNT          580 a          524 c          454 g          536 t
ORIGIN      
        1 taaggacttc aagcacagaa aaccatcatt ttccttgaaa aagaactggg aactttgtta
       61 ccaaaccaag gcagtattga tttccaattg atcttaatgg aatcaaggct tttgattttt
      121 tcgggtattc tcttttccac attagtgaca cagtttatga ccaacttatc taactcacac
      181 ctctcccaaa tcacatcgct tctggaaaag gcctgtcatc agggtatcat tcagggggct
      241 caatggatgg cgctcaaaaa tgggattgtc cacggggcgg gtcaagctgg acaggcatat
      301 gaaatctatg atctggcgtc ccttaccaaa cctctggcaa cggcccttgc ctgccttata
      361 ttaaaagatc aggggaaatt agacctggat actcctgtat cctcatactt gagtgagctg
      421 gattcaccgg aaaaaaaaag catcacggta cgccagatac tgatacatac tgcaggtttt
      481 gctgcctggc gcccatttta cctgctggct tgtcatccag atgaaattat tgcgactatc
      541 aaccagatcc ctctggaata tgaagctggt tccaaagtgg tttatagtga ccttgggtat
      601 attgtgcttg gaaagatgct tgaaaaaatt actatcaccc ccatggagaa actctttcaa
      661 tttttggtcg ctgcccccct gaacctgaaa aatttaaggt attgccctcc gaaatcctgg
      721 aaacctcgca ttgctcctac tgaaatcgga aatcaatatg aacagggttt agctgttcgg
      781 actatcggcc aggattggcc gacaacccat cagtggcgaa cgaccctgat ccagggtgag
      841 gttcacgatg caaatgcttt ctttttaaat gggggtgcag gtcatgccgg actctttggt
      901 tcaatccagg atgtggccgc tttaagtcat caatttctac ctggaagcca gttacttcag
      961 ccatcaacat tgaaggaatt ttgccacaat gatactccag gacagaacga agcccggtca
     1021 cctgggtgga tgctcgctca aacaccccag tgtagtgcag gaaacacctg ttctcctcag
     1081 acttttggtc atgttggatt tacagggaca agtctgtgga ttgacccgga atcaatgaca
     1141 gtgacgatcc tgcttgcaaa ccggactttt ccgatacagg atttaactcc gcttcgtcgg
     1201 cagttccact ctctggcgac aactctttca aataaagatt agaacacagg gagggtccag
     1261 cacctgtact ctcttttgcc cattccctcc attgagatta ctctccgccc catttcagct
     1321 tatctcgcag gatttggaag taatttcgat ttggaggctg aattaatttc aagactgaat
     1381 cacttttgcg aatgacgatc cggtcatcaa caaacagcga aatcgcaatt tggccatcca
     1441 gggtcagagt aacatcttcg atatccccac gagtcgcaac cagatgaagc tcaatcactg
     1501 attcgtctga aaccaccaga ggtcgattgg tcagggtatg ggggcaaatt ggagtaataa
     1561 ccacagcatg catcgcgggg tgaataatcg gccctccggc tgaaagcgag taagcagttg
     1621 aaccagttgg agttgaaatg attaacccat ccgcccgaaa catggaaaca aactggccat
     1681 caatccagca ctgaatctca ataatccgcg ccagtaacgc cttattgacg acgacatcat
     1741 tgataacttc aactcgggaa acttcatgcc cattgcgtag aaccgccgcc tcaagtttga
     1801 ttcgggtgtc cacctgatgt ttcccagcca ggacaagttc cagggccgga aaaatattat
     1861 ctggcgaaaa ttcagtgaga tagcccagat agccaaaatt taccccgagg atacaagtcg
     1921 gacgcttgcc aacaattctg gctccagtaa tcatcgtgcc atcaccacct aaaacaacga
     1981 ttagatcaga gcattcggca agttcttgat ttgaaacgat ttttactgat ggtgggaaca
     2041 cgctatcccc cagcccatca gccaaaacct gaccccctcg atgtaccagc catt
//
LOCUS       DAOQDO010001169         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51340_length_2093_cov_3.154563, whole genome shotgun sequence.
ACCESSION   DAOQDO010001169 DAOQDO010000000
VERSION     DAOQDO010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51340_length_2093_cov_3.154563"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKZ53_16950"
     CDS             <1..318
                     /locus_tag="PKZ53_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42179.1"
                     /translation="NTLMCGDLEIGQLEVCGVDNFWTYGRFHPLPGYARYSDLFGLLE
                     TAWSRSEPAVTDSDDVFEIQDKVNLLELQVRTQTGILHRIRDFKIQNGQYEYKLDLGE
                     ILT"
     gene            complement(384..469)
                     /locus_tag="PKZ53_16955"
     tRNA            complement(384..469)
                     /locus_tag="PKZ53_16955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Ser,seq:tga)
     gene            complement(507..1244)
                     /locus_tag="PKZ53_16960"
     CDS             complement(507..1244)
                     /locus_tag="PKZ53_16960"
                     /inference="COORDINATES: protein motif:HMM:NF017779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42180.1"
                     /translation="MLKDAVGWTLHDAIELISPLGMSQEARPKTFRHAIFGESAKMLQ
                     VTSDGWILAGGRLNLKYSRIQSIVADVATLMVYPTASPELLPIFASEWVVVGERAHVL
                     VLDVEFAGQQPHLKAQLTPVFQPLAERFGPRFEAIAERPGWFQEIAEPWAIFASGFVS
                     VLVDVREAFNTYLQATIQSAYLPNLSQAKAGEDHPDVAHYKHHHYIHSPGIPMIQPKA
                     GEAWTHEFMRDWHFGPPRFPDLPEIFA"
     gene            complement(1364..>2093)
                     /locus_tag="PKZ53_16965"
     CDS             complement(1364..>2093)
                     /locus_tag="PKZ53_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ42181.1"
                     /translation="PPPQWKTMKPEAKSFVVQKITQELADVRSYTFHFGLMRFQFEPG
                     QFVVASLDSKGIDAQGALTLSSSPLHPTAFELTVKRSGGFGTTFYDTVEEGDIISIGS
                     AKGPFMLPREGTQPVCFISRDYCIPAARSYYRFLTDTKATRPFLLLHEITNPTQILYH
                     QEFTRPAWNGFARLIFLDSPTKPANWTGPHTRITRSIIQRIIKRPDDTLFFITGEGPD
                     IKYYKEQLEGLNLPPRNIHYERWS"
BASE COUNT          528 a          488 c          574 g          503 t
ORIGIN      
        1 aataccttga tgtgtggtga tcttgaaatt ggccagcttg aagtgtgtgg ggttgacaac
       61 ttttggacgt acggtcgctt ccatccgctt ccaggttatg caaggtactc tgatttgttc
      121 ggcctgctgg aaactgcctg gtccaggagt gagcctgccg tgactgattc tgacgatgtg
      181 tttgaaatcc aggacaaggt gaatttgctt gaactacagg tgagaaccca aacaggtatc
      241 cttcacagaa tccgtgattt taagattcag aatggccagt atgaatataa gcttgatttg
      301 ggtgagatac tgacctgagc ggtaaaaatg aaaaagccac gaactggtcg tggcttacaa
      361 aaaactctat ctgagaaatt gtgtggcgga ggcagaggga ctcgaacccc caagcggttc
      421 cccgcgccag ttttcaagac tggtgactta ccaattagcc tatgcctccg gggaatttgg
      481 gataatagtc agactcgtct cgaagatcaa gcaaaaattt cgggcaggtc cggaaatcgg
      541 ggcgggccga aatgccagtc gcgcataaat tcgtgcgtcc aggcttcacc agctttgggc
      601 tggatcatcg ggattcccgg cgaatgaata taatgatggt gtttgtaatg tgcgacgtct
      661 ggatgatctt caccggcttt ggcttgagac agatttggca ggtaggcgga ttgaatcgtg
      721 gcctggaggt aggtgttgaa ggcttcacga acgtccacca gcacgctgac gaaacccgag
      781 gcaaagatgg cccagggttc ggcgatttcc tgaaaccagc ctgggcgctc ggcgatggct
      841 tcaaaccgtg ggccaaagcg ttctgccaga ggttgaaaaa caggcgtcag ttgagctttt
      901 aagtgtggct gctggccagc aaattcaacg tcaagcacca gcacgtgggc ccgttcacca
      961 accacgaccc actctgacgc aaaaatcggc aggagttccg ggctggctgt cgggtacacc
     1021 atcaatgtgg ccacatcagc cacaatcgat tggatccgcg aatatttcag atttaaccga
     1081 ccaccagcca gaatccatcc atcactggtg acctggagca ttttggctga ttcgccaaag
     1141 atggcatggc gaaaggtttt ggggcgagct tcctggctca ttcccagtgg cgaaatcaat
     1201 tcaattgcat cgtgcaacgt ccagccaacc gcatctttga gcatttggaa atcaccttgc
     1261 tgatagggtt ccgggttccg gggaaatcaa aagacaaaaa ggacgaaaag gacataaatt
     1321 tcgaagaacc cggaacccgg aacctggaac ccggaacccg gactcagctc caccgctcat
     1381 aatgaatatt gcgtggcggc agattcagac cttcaagttg ttctttataa tacttaatgt
     1441 ccgggccttc gccagtaata aaaaacaatg tgtcatctgg tcgcttgatg attctctgga
     1501 tgatcgagcg ggtaatgcgg gtatggggac cggtccagtt ggctggtttg gttgggctat
     1561 ccagaaagat cagtcgggca aatccattcc aggctggtcg agtaaattcc tgatgataca
     1621 ggatttgagt gggatttgtg atttcgtgga gcagcaaaaa tggtcgcgtt gctttggtat
     1681 cagtcaggaa ccggtaatag ctgcgggcgg ctgggatgca gtaatcccga ctgatgaaac
     1741 aaaccggctg ggtgccttcg cgtggcagca taaacgggcc tttggctgac ccaatggaga
     1801 taatatcccc ttcttcaact gtgtcataaa acgtcgttcc aaatccgccg gaccgtttta
     1861 ctgtgagttc aaaagcggtt gggtgcagtg gggatgaact cagggtcagc gctccctgag
     1921 catcaatgcc ttttgaatca agactcgcca ccacaaattg tcccggctca aactgaaatc
     1981 gcatcaaccc gaaatgaaag gtatagctgc ggacatccgc cagctcctgg gtaatttttt
     2041 gaacgacaaa cgattttgcc tcaggtttca tagttttcca ttgcggggga ggt
//
LOCUS       DAOQDO010001170         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51399_length_2092_cov_2.305351, whole genome shotgun sequence.
ACCESSION   DAOQDO010001170 DAOQDO010000000
VERSION     DAOQDO010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51399_length_2092_cov_2.305351"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="PKZ53_16970"
     CDS             <1..1984
                     /locus_tag="PKZ53_16970"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ42182.1"
                     /translation="VDFQPDTSADDMAEKLIAETLTDPGIVEIGYVAEQRWTVGLREI
                     PVENGEPGMELTKDTVFVVTGAAGSIVSAITTDLAVASGGTFYLLDLVPEPDRNNPDI
                     PRLANDKDNLKRDIFERLKAKGERATPAMVEKELAALERAGAALAAIQAIEAAGGTAH
                     YFSVDLRDAESVGNAIDAVQEQQGRIDVLLHAAGLEISRFLKDKEPREFDLVFDVKCD
                     GWFNLLRAIGQMPLGATVVFSSIAGRFGNGGQTDYSSANDLLCKTTSSFRTTRPNTRG
                     IATDWTAWSSIGMASRGSIPKMMELAGIDMLAPEAGIPFIRRELISGSRRDEIVVAER
                     LGLLMHEFDETGGLDVEAINQRLAEHREAGKVGPMIGNVTGFGLHTGLTVETTLDPKE
                     QPFLFDHQIEGTPVLPGVMGMESFAELAAVLFPDQSVASVENVDFLAPFKFYRHEPRT
                     IKLVAKFQTDGEDTLAFCRLIGTRTLPNQPTPQETVHFTGTVRLTRQPLSEDLEQPAT
                     EAEATVTAEDIYRIYFHGPAYQVLEKIWKWEDCVAGQFAADLIENHRPVELETQLTPR
                     LIELCFQTAGIWEIGETGHMGLPLHVDRVLKLRTGENVSGRQVAIIRRNTENNCFDGV
                     VCDTEGNVFLYLQGYRTVEFTANDEAQVALIRNVLS"
BASE COUNT          496 a          612 c          562 g          422 t
ORIGIN      
        1 agttgacttc caacctgaca cttcagccga tgacatggct gaaaaactga ttgctgaaac
       61 cctgactgat cctggtatcg tcgaaattgg ctatgtggct gaacagcgct ggacggttgg
      121 actccgggaa atcccagttg aaaacggcga acccggaatg gaattgacca aagacaccgt
      181 gtttgtggtc accggtgccg ccgggagcat tgtgtcagcg attacgaccg atctggccgt
      241 ggcctcaggc gggacattct atctgcttga tttagtcccc gaaccagacc gcaacaatcc
      301 agatattcca cgactggcca atgacaaaga caacctcaag cgagacatct ttgaacgcct
      361 caaagccaaa ggcgaacgcg ccaccccggc catggttgaa aaagaactcg ccgccctgga
      421 acgcgctggt gccgcacttg ccgccatcca ggccatcgaa gccgccggcg ggacggctca
      481 ctatttcagc gttgacctgc gggatgccga atcagtcggc aacgccattg acgccgttca
      541 ggaacagcag ggccggattg atgtgctgct gcacgccgcc gggcttgaaa tcagccgctt
      601 cctgaaagac aaagaacctc gcgaatttga tctggtgttt gacgtcaaat gcgatggctg
      661 gtttaacctg ctgcgcgcga ttggtcagat gccgctgggt gccaccgtgg tgtttagctc
      721 gattgccgga cgctttggca acggcggtca aaccgattac agctctgcca acgatctgct
      781 ctgcaaaacc acatcaagct tccgcacgac gcggcccaac actcggggca ttgccactga
      841 ctggacggcc tggagcagca tcggaatggc ctcgcgcggc tcgattccaa aaatgatgga
      901 acttgccggc attgatatgc tcgctcctga agctggtatc ccgttcattc gtcgcgaatt
      961 gatctctggc agccgccggg atgaaatcgt ggtcgcagag cggcttggcc tcctgatgca
     1021 tgagtttgac gaaaccggcg gtctcgatgt cgaagccatc aatcaacgac tggccgagca
     1081 ccgcgaagcc ggaaaagtcg gcccaatgat tggcaacgta accggatttg gactccacac
     1141 cggactcacg gtcgagacca cgctggatcc aaaggaacaa ccattcctct ttgatcacca
     1201 gattgaaggc acgccggtac taccgggcgt gatgggcatg gaatcatttg ccgaactggc
     1261 cgccgtgctc ttccctgacc agtcagtcgc cagcgttgaa aacgtagact tcctggcgcc
     1321 atttaagttt tatcgccacg aaccacggac catcaaactt gtagccaaat tccaaaccga
     1381 cggcgaagac acactggctt tctgccgcct gattggaacg cggaccttgc caaaccaacc
     1441 gacgccgcag gaaaccgttc actttaccgg cacagttcgc ctgacccggc aaccgctctc
     1501 ggaagacctg gaacaaccag caactgaagc tgaagccacg gtcaccgccg aagacattta
     1561 ccggatttat ttccacggcc cggcctatca ggtccttgaa aaaatctgga aatgggaaga
     1621 ctgcgtggcg ggtcagtttg ccgccgatct gatcgaaaac catcgtccag tggaactcga
     1681 aacccaactg acaccgcgtc tgatcgagtt gtgcttccaa accgctggaa tctgggaaat
     1741 tggcgaaacc ggccatatgg gcttgccact gcacgttgat cgggtgctca aactccgcac
     1801 cggagaaaat gtcagcggtc gtcaggtcgc catcatccgt cgcaacaccg aaaacaattg
     1861 ctttgacggc gttgtctgcg acacggaagg taatgtcttc ctgtacctgc aaggctatcg
     1921 gaccgtcgaa tttaccgcca acgatgaagc gcaggtcgcg ctcatccgca atgtgttgag
     1981 ttgatgtctc agtcagtacc acctgcgtca gcgggtgggc tcgttgagct gatgtcctag
     2041 tcagtaccac ctgtgtcagc gactggaagt gctgaaactg aacaagccca cc
//
LOCUS       DAOQDO010001171         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51495_length_2090_cov_3.200983, whole genome shotgun sequence.
ACCESSION   DAOQDO010001171 DAOQDO010000000
VERSION     DAOQDO010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51495_length_2090_cov_3.200983"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..>2090
                     /locus_tag="PKZ53_16975"
     CDS             505..>2090
                     /locus_tag="PKZ53_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein kinase family
                     protein"
                     /protein_id="HNJ42183.1"
                     /translation="MATLRKAGDFIGPGEQTTAAYLEKQLPPSWVIIANKELVRPDGS
                     VRELDFIVMGEHCLFAVEEKSWSGPIHGDENGWVLRSGASYRNPIDVVEMNARRLAGI
                     VQDRVPVLKHQVSGHFVYSRVLLSDSNADPSFIHDPRINTHVLALDGCEEEFLRIDQH
                     QAGPGSINAYRKKIVDQLIGLPDRPRIPRKVGEFEILEVVSTTDTVRCLRARHEDGSE
                     RLLKLVERPVTAISELLEEQTNLIKREYEALRLLAQKGCVPQIDPYFSWSDGQFWVFP
                     HHPIPGRTLRATRTSQPPTQKQLWIVFEQAFSALADIHETGTLHRAITPDSLYLTDDQ
                     QIVFTDFALARIRNRNTIAPILDVEAGQSPYQAKECQEVGLGFAEPQSDVFSLTASLL
                     YWATGFEPPAFGSMVGSLPDDFQQALGSILTQCLEIDDERRRPLASEVVQAIQKARSN
                     DMSPQFSSVPTPVPAGTLRPGTEVENQYRIVRKLGEGGTAITYLADDLVTDSRYVLKT
                     IKSPELITRLSKAEFAALKE"
BASE COUNT          539 a          539 c          500 g          512 t
ORIGIN      
        1 ctgattcaac cgttttcttt cttgaaaaaa cagcttgaca tagaatgtcc ggctatccga
       61 acggcagcgc ttcgcgatgc aaaaccaaaa acctacacgt gaaaggtctc agtcgcccgg
      121 tactccccca caaatggatt gaaaattcat ccactttcct ttagctttcc aaagcccaga
      181 aaaataacca gccctgtcat cctgtgatca attaccatca atccagtcag cacaagatat
      241 atcttgtcaa tacaagaaat atctctgttt cccgctggtt tcagttgatg aaggaatggg
      301 ttctttacat atcagtgccc tctgatgtga tttttcacca atttcagata acactttgat
      361 ttcaaatcgt ttgaccacaa cctctactgc ttgaaaacac ccgatgggtg cttttgcgcg
      421 gattgtatca attgacagca acgcccacct caatccagtc catctgcaat ggtaaacttg
      481 tggaaggaaa ggagtttttt cgtcatggcg accttgagaa aagcggggga tttcattggc
      541 cccggagaac aaaccacagc ggcctacctg gaaaaacaat tgccgccatc ctgggttatc
      601 attgccaata aagaactggt tcggcccgat ggcagcgttc gcgaacttga tttcattgtg
      661 atgggtgaac attgcctgtt tgcggttgaa gaaaaaagct ggtcaggtcc aattcacggc
      721 gacgaaaatg gatgggtttt gcgcagtggt gccagctatc gcaatccaat tgacgttgtt
      781 gaaatgaatg cccggcgtct ggcgggcatt gtgcaagatc gagttcccgt cttaaagcat
      841 caggtgtcgg gtcattttgt ttatagccgg gttctacttt cagattccaa cgcagatccc
      901 agctttattc atgaccctcg aatcaacacc cacgttctgg cattggacgg gtgcgaagaa
      961 gagtttcttc ggattgacca gcatcaggcc ggcccggggt caatcaacgc ctatcgcaag
     1021 aaaattgttg atcagttgat cggattgcct gaccgcccca gaattcctag aaaagtcggt
     1081 gagtttgaaa tcctcgaagt ggtatcaacc actgataccg ttcggtgtct gagagcccgc
     1141 catgaagatg gatcagagcg attgctcaaa ctggtggaac gcccagtcac agccatttca
     1201 gagcttttgg aagaacaaac aaatctgatc aagcgcgaat atgaagccct gcgtctgctg
     1261 gcacaaaaag gatgcgttcc ccagattgat ccctattttt cctggtcaga tggacaattc
     1321 tgggtttttc cacaccaccc gatccccgga agaaccttgc gggcgactcg caccagccag
     1381 cccccaaccc agaaacaact ctggatcgtg tttgaacaag cattttcggc gttggctgac
     1441 attcatgaaa cgggaaccct tcaccgggcc attacacccg attcactcta tttgaccgat
     1501 gaccagcaga tcgtgtttac cgattttgcg ctggcgcgca tccggaaccg aaacacgatt
     1561 gcaccgatac ttgatgtgga ggcgggtcaa tcgccatatc aagccaaaga atgccaggag
     1621 gtcggacttg gatttgccga accgcaaagc gatgtgttta gtctgactgc ctcgttgctg
     1681 tattgggcca ccgggtttga accacctgcg tttgggtcaa tggttggttc acttccagat
     1741 gatttccaac aggcattggg cagcatcttg acccagtgtc tggaaattga cgatgaacgg
     1801 cggcggccat tggcttcaga agtggttcaa gcgattcaga aagcacggtc aaacgacatg
     1861 tcgccgcagt tttcctctgt tccgacaccc gtcccggcag gaacactccg accaggaacc
     1921 gaagtggaaa accagtaccg gattgtccgt aaactgggcg aaggcggcac cgccatcacc
     1981 tatctggctg atgatctggt gactgactcg cgatatgtgc tcaagaccat caaaagccca
     2041 gaactcatca cgcggctttc caaagctgaa tttgcagccc tgaaagagtt
//
LOCUS       DAOQDO010001172         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51501_length_2090_cov_2.807371, whole genome shotgun sequence.
ACCESSION   DAOQDO010001172 DAOQDO010000000
VERSION     DAOQDO010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51501_length_2090_cov_2.807371"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKZ53_16980"
     CDS             <1..571
                     /locus_tag="PKZ53_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42184.1"
                     /translation="NYSNLDPNLPLTRYGIGHWLEVVDPATGAVVEHSSQGAFGCSPW
                     IDYRRNLVGVFLTFSVLNRVMPTYLKLKDTIHSIVPASTADLIVPVVSDVTTSKAKVF
                     RPKDPMIEIRWKSVDNIGVVGHDLSYASDGTSFSTAVATGLSGELQTYTWTIPPSQPR
                     SKSARIKVSARDAAGNIGDGITRGVLIVK"
     gene            702..1982
                     /locus_tag="PKZ53_16985"
     CDS             702..1982
                     /locus_tag="PKZ53_16985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ42185.1"
                     /translation="MKIPGILCILLLLFSTGQPQTSQLFAGQKPVKSKPSDLALVGAK
                     IYPSPSENPIEQGVIVIRNGKISAIGELGKVKLPAKITTLDCTGMTITAGFWNCHVHF
                     SELKWEESRTLPAAQLTGQLQEMLTRYGFTSVFDTGSYWEITSSLRQRIESGEVDGPQ
                     IFTTGEILFPKNGVPPLEAVQAAGGIARPMPEAQTSEQAVQLVTQKLKAGTDGVKLYA
                     QTFWNPNLKIPVDIIKAVTAEAHRQHKPVFVHPSNQYGLEAAIESGVDVLVHTTPQTG
                     PWDATIVGKMKQAKMALVPTLTLWHIELEREGAPPEDQKGFQTRGVNQLRAYFQAGGQ
                     ILFGTDVGYIPVYDTTEEFEQMERAGMRFSDILAALTTAPAGRFGKPNQTGKIAAGTL
                     ADLVVLESDPGQNIRAFSKVKFTLRNGKIIFQSK"
BASE COUNT          543 a          558 c          502 g          487 t
ORIGIN      
        1 caattattca aatctggacc cgaaccttcc gctcacccgt tatggcatcg gtcactggct
       61 ggaagtggtt gacccggcaa cgggtgccgt cgtggagcat agttcacaag gtgcttttgg
      121 atgcagtccc tggattgatt accggcgcaa tctggtcggc gtcttcctga cgttcagtgt
      181 gctcaaccgg gtcatgccaa cttacctcaa actcaaagac accattcatt caatcgtgcc
      241 ggcttcaacc gctgatctga tcgtgccggt tgtgtctgac gtcacgacgt caaaagccaa
      301 ggttttccgc ccgaaagatc cgatgattga gattcgctgg aagtcagttg acaacatcgg
      361 tgtggtaggt cacgaccttt cctatgcctc agatggcacg tcgttttcaa ctgccgtagc
      421 gacaggttta tccggtgaac tacaaactta cacctggacg attccaccca gccagccccg
      481 ctcaaagtct gcccgaatca aagtttctgc ccgtgatgcg gctggaaata tcggtgacgg
      541 catcacccgt ggggttttga ttgtgaagtg atccagggtt gatttcactt tttcccatac
      601 ctgttctgaa aaaaagttgg ttgattcaac ttctggctgt tgatcggcgt acgggcaccc
      661 tcacaccaaa tccaatccga ctcagataaa ggtgcccgtc tatgaaaatt ccaggcatat
      721 tgtgcatcct gttactgcta ttcagtacgg gacaacccca aacctctcaa ctctttgccg
      781 gccaaaagcc agtcaagtca aaaccatctg atctggcgct ggttggcgca aagatttatc
      841 cttcgccatc tgaaaatccg attgaacaag gcgtgatcgt catccggaat ggcaaaatca
      901 gtgcgattgg cgaactcggc aaagtcaaac ttccagccaa aatcaccact ctggattgca
      961 ccgggatgac aatcacggct ggattctgga attgtcatgt tcattttagt gaactgaaat
     1021 gggaagaatc caggacgctt ccagcggcgc aactgaccgg gcagctccag gaaatgctga
     1081 cccggtatgg gtttaccagt gtttttgaca ccgggtctta ttgggaaatc accagttcgc
     1141 ttcgacaacg gattgaatcg ggcgaagtgg acggcccaca gattttcacc accggagaaa
     1201 ttctctttcc gaaaaacggg gtgcccccac ttgaagcggt tcaagcagcg ggcggaattg
     1261 ctcgaccaat gccggaagct caaacttcag agcaagctgt ccaactggta acccagaaac
     1321 tgaaagctgg gacagatggg gtcaaactgt atgctcaaac cttctggaat ccaaatttaa
     1381 agatcccggt tgacatcatc aaagctgtca cagccgaagc ccaccgccag cacaaacccg
     1441 tctttgttca tccgtcaaac cagtatggac tcgaagccgc gattgaaagc ggcgtggatg
     1501 ttctggttca caccacccct caaaccggcc cctgggatgc aactattgtt ggcaaaatga
     1561 aacaggccaa aatggcgttg gtgccaaccc tgaccctctg gcatatcgaa ctcgaacgcg
     1621 aaggcgctcc gcccgaagac cagaaaggat tccaaaccag aggcgtcaat caacttcgag
     1681 cctattttca agctggtggt caaatcctgt ttggcaccga cgttggctat attccggtct
     1741 atgacaccac cgaagaattc gaacaaatgg aacgtgctgg catgcgcttt tcagatattc
     1801 tggcggcctt aacgacggcg cctgccggac gatttggaaa accaaatcaa accgggaaga
     1861 ttgcggctgg cacactggct gatctggttg tactggaaag tgatcctggg caaaatatcc
     1921 gcgctttttc aaaagtaaaa ttcacgttgc ggaatggaaa aattattttt cagtcaaagt
     1981 aatcccagcc cacaaccgtc aggctttccc ccttcaccca gtttcgaatc tgaacattga
     2041 gaggtaaccg atgagtactc cgacaaccaa gaaaattacg actgccgatg
//
LOCUS       DAOQDO010001173         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51503_length_2090_cov_2.587715, whole genome shotgun sequence.
ACCESSION   DAOQDO010001173 DAOQDO010000000
VERSION     DAOQDO010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51503_length_2090_cov_2.587715"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKZ53_16990"
     CDS             complement(<1..690)
                     /locus_tag="PKZ53_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42186.1"
                     /translation="MKLPRRHGLFLFGIGLVCSGFWLHLTSTEAARPVSTPQVSSTVQ
                     PAAIWQDISESGFSKTTQLQSQRQIIPTTYRTLLLDKTTFSQTLQTVPLETNRTPEVA
                     PVILAFPLPDKTFARFAVWESPIMATELAAEYPAIRTYAGRGIDDPTASIRFDQTPLG
                     VHAMMISAQGTFYIDPYAKGNTELYICYNKRDFHKPAADLARCLIDPAQSKKRPSSGF
                     SPLQADTTPPPS"
     gene            1161..>2090
                     /locus_tag="PKZ53_16995"
     CDS             1161..>2090
                     /locus_tag="PKZ53_16995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ42187.1"
                     /translation="MKSQFRWVSSVLFAVVCVMCLTSLCLPPISAQSGRKRSSPPVKA
                     NTQDGSSSTPRKEEVIMAGADDPALQDAEVLKIDATLITVPAVVSDNNGYYLPFLRKE
                     QFRLFEDGTEQDISFFAPERVPFHVALILDMSGSTVLSAPDIQTAARAFVNHMRDDDK
                     VAVVTFASSIELLCDFTDDRQVLENAIERTRSGGSTKLYEAVYKTVRNYMRNIEGRKA
                     IILLTDGEDTSSKGVSYRDAIDAVVESDVLTYVIQYPASDWSGQTGAPGRPTWFPFPL
                     PWPRRPQGTPPIGWPLQASFTPPQGRFPQTTQPY"
BASE COUNT          515 a          519 c          558 g          498 t
ORIGIN      
        1 tgatggaggt ggggttgtat ctgcctgcaa cggggaaaat ccactggatg gacgcttttt
       61 ggactgtgcc gggtcaatca ggcaccgtgc caggtcagcc gccggtttat ggaaatcacg
      121 cttgttatag caaatgtaca gctcggtgtt tcctttggca tacgggtcaa tatagaaggt
      181 cccctgagca gaaatcatca tggcgtgaac accaagcggt gtctggtcaa aacgaatact
      241 ggcggttgga tcatcaattc ccctgccagc ataggtccga attgccgggt attcggcggc
      301 cagttcagtc gccataatcg gagattccca gacggcaaac cgggcaaacg ttttgtctgg
      361 caatggaaac gccagtatca ccggcgccac ctctggggtt cgattggttt caagtggaac
      421 agtttgaagg gtttgggaga acgtagtctt atccaaaagg agcgttcgat aggttgtcgg
      481 aataatttgt cgctgtgatt ggagttgagt tgtttttgaa aaacctgatt cagaaatatc
      541 ttgccagatg gcagccggtt gaacggtgga tgaaacctga ggcgtagaca ctggtcgggc
      601 agcctcagtc gaagtgagat gcagccagaa cccactgcac acgagaccga taccgaagag
      661 aaaaagcccg tgtcggcgtg gcaatttcat acgaaagacc tccgaaaggt ggtggaatat
      721 cagtaagaga atgtgatctg gaaataacat cgggcaattt gcagaagacc acttaaattc
      781 atcatttcgg aagatttatg cagaggttct tcctgatgga ttcggcaatc agaagtgagt
      841 cacactgaaa catgcaagcc gggaatatca aacaaattgg atgtatctca ggatcgtggc
      901 gaaagatggc gtgtttctga aacaggatcg ggaagcattg aaccaatact cttcttttcc
      961 ttccattgca atgacgtgca tgacagggtt aggaaagctc agcttctgat caaattcacc
     1021 aacctgggtg gccgccgaag ccaaaaccgg tataattttt caacaccacc gaaacaacac
     1081 ttctggattc aggtagcatc agacccgcag cctgtctctt ccgcctttcc aatttcgcca
     1141 tcactacccc ggggttctct atgaaatcgc agttcagatg ggtgtcgtct gtcttgtttg
     1201 cggttgtgtg tgtcatgtgt ttgacctcac tgtgccttcc tccaatctcg gcccagtcgg
     1261 gcagaaaacg atcttctcca ccagtcaaag ccaataccca ggatggttcg tcatcaacgc
     1321 ctcgcaaaga agaagtcatc atggctgggg ctgatgaccc ggcactgcaa gatgctgaag
     1381 ttttaaaaat tgacgccacg ttgattaccg tcccagcggt tgtcagtgac aacaacgggt
     1441 attacctgcc ttttttgcgg aaagaacaat tcagattgtt tgaggacggc acggaacagg
     1501 acatttcgtt ttttgccccg gaacgagtgc cattccatgt cgccctgata ctcgatatga
     1561 gtgggagcac ggtactgagt gcgccagaca ttcaaaccgc cgcccgggcg tttgtaaatc
     1621 acatgcgcga tgacgacaaa gtggcggtgg tgacgtttgc cagttcgatt gaactgctgt
     1681 gtgacttcac tgatgaccgc caggttttgg aaaatgcgat tgaacgcacc cgctctggcg
     1741 gcagcaccaa actgtacgaa gcggtgtata agacggtgcg caactacatg cgcaacatcg
     1801 aaggtcgcaa agctattatc ctgttgacag atggtgagga cacctccagc aaaggcgttt
     1861 cctatcgcga tgcgattgat gcggtggtcg aatctgatgt gttgacctat gtgattcagt
     1921 acccggcaag cgactggtcg ggccagactg gggctcctgg acgccccacc tggtttccct
     1981 tcccactgcc ctggccgcgt cgtccgcaag gcaccccgcc gattggctgg ccgctccagg
     2041 cttcattcac gccaccgcaa ggtcggtttc cacaaaccac ccagccttac
//
LOCUS       DAOQDO010001174         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51550_length_2089_cov_2.808751, whole genome shotgun sequence.
ACCESSION   DAOQDO010001174 DAOQDO010000000
VERSION     DAOQDO010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51550_length_2089_cov_2.808751"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PKZ53_17000"
     CDS             <1..750
                     /locus_tag="PKZ53_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016956046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ42188.1"
                     /translation="AGFVPVDFWDNVRRELDLIKPVFMLAEWESRDLHARAFDMTYAW
                     SWNETMHKIAKGQADVNALFIYYSWNESSFPPNSMRMTFVSNHDKNSWEGTEFEQFGD
                     CLEAAIVLSVVGEGLPLMYNGQEAGNPRRLKFFEKDPIEWKEHPLGDLYKKLFALKKK
                     NTALWNAKWGATMIKVPNTVPTKVFSFIRQNEKEKVFAVMNFSDKPQTVTFSETLYHG
                     KYTDYFSGETVEFPTTTQMQLKPWAYRVFVK"
     gene            912..1478
                     /locus_tag="PKZ53_17005"
     CDS             912..1478
                     /locus_tag="PKZ53_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNJ42189.1"
                     /translation="MTELNQTAYDAMRESPKDEGTLRMIVRRPAVDAREVLETGQLDI
                     AEGLVGDTWNQRHSPRTPDGSPHPDTQINIMNARVIALLAGSQDRWSLAGDQLFIDLD
                     LSLENLPAGTQLEIGEALLVVTDQPHTGCKKFSERFGPDALKLVNTPEGRQLNLRGIN
                     ARVVRSGRIQIGETVRKICHQASAATSD"
     gene            1500..>2089
                     /locus_tag="PKZ53_17010"
     CDS             1500..>2089
                     /locus_tag="PKZ53_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNJ42190.1"
                     /translation="MKKAFLFAGIGFGLIVLVVGVAFLFARQPAESKPIPSVGGTPQL
                     STGSGTTQLATTVGTIPYYLQHDPKWGSQTIGGSQESMAAVGCTITCVAMGLSALGHP
                     VDPSQVCDQLKNRNGFTERGYLIWSKVGEITSGKIQIGIPEVSHAVIDAELVKRRPVI
                     VKILLAPTIPHWVLIVGKEGQEYLVMDPLNDDKRLV"
BASE COUNT          550 a          535 c          543 g          461 t
ORIGIN      
        1 gcgggtttcg tcccggttga tttctgggac aatgtccgcc gcgaactcga cctcatcaaa
       61 ccggtgttta tgctcgccga atgggagtca cgcgacctcc acgccagagc ctttgatatg
      121 acctacgcct ggagttggaa tgaaaccatg cacaagatcg ccaagggtca ggcagacgtc
      181 aacgcgctgt ttatttacta ttcctggaat gaaagttcgt ttcctcccaa cagcatgcgc
      241 atgacctttg tcagcaatca cgacaaaaat tcgtgggaag gcactgagtt tgaacagttt
      301 ggcgattgcc tcgaagccgc gattgtgctc tcggttgtcg gtgaaggatt gccgttgatg
      361 tacaatggtc aggaagccgg aaacccgcgt cggctcaaat tctttgaaaa ggatccgatt
      421 gaatggaaag agcatccact cggggacctc tataaaaagc tcttcgcact caagaaaaag
      481 aacaccgccc tctggaacgc caaatggggt gcgaccatga tcaaagtccc caatactgtc
      541 ccaaccaaag tctttagctt tattcgccag aatgaaaaag aaaaagtctt tgccgtgatg
      601 aacttttctg ataaaccaca aaccgtgacc tttagtgaaa ccctgtatca cggaaaatac
      661 accgactact ttagcggaga aaccgttgag tttccgacta caacccaaat gcaattaaaa
      721 ccgtgggcct atcgggtctt tgttaaataa gagatggctg gaaatcctgg ggctgagggc
      781 ttggggcaaa ggaaaggatg agagatgaag gatgagggat gaaaaaaacc aattcttcag
      841 ctcgactgct tcagcccgaa gtcttcagcc cgaagtcttc agcccgaagt cttcaaccct
      901 aagcgaggat tatgactgaa ctgaatcaaa ctgcatatga tgctatgcgt gaatccccca
      961 aagatgaggg cacgctccga atgattgtcc gccgccctgc cgtggatgca cgcgaagtcc
     1021 ttgaaacagg tcaacttgac atcgccgaag gactggttgg cgatacctgg aaccagcgac
     1081 atagcccccg aacgccggat ggctcgccac atcccgacac gcaaatcaat attatgaatg
     1141 cccgcgtgat tgcgcttctg gcagggagtc aagatcgttg gtccctggcc ggagaccagc
     1201 ttttcattga tctggatttg agcctggaaa acctgcctgc tggaacacaa cttgaaattg
     1261 gcgaggcact gctggtcgta accgatcaac cccataccgg atgcaagaaa ttttcagaac
     1321 gatttggccc tgacgcgctc aaactggtga atacacctga aggccgccag ctcaatctgc
     1381 ggggaatcaa tgcgcgagtg gttcgttcgg ggcgcatcca gatcggagaa actgtcagaa
     1441 aaatttgcca ccaggcatca gccgcgacat cggactagcc ttgccatcga ggtgacccta
     1501 tgaaaaaagc ctttctcttc gcaggcatcg ggtttgggtt gattgtcctg gttgtgggag
     1561 tcgcctttct gttcgcccgt caaccagccg aaagcaagcc gataccatct gttggcggta
     1621 cacctcaact cagcaccggg agcggaacga cgcaactggc gacaacggtt gggacaattc
     1681 cctattattt gcaacacgat ccaaaatggg gttcgcaaac catcggcggc agccaggaaa
     1741 gtatggccgc tgtcgggtgt accattacct gtgtcgccat ggggctttca gcacttgggc
     1801 atccagtgga cccatctcaa gtctgtgacc agttgaaaaa tcggaacggc ttcaccgagc
     1861 gtggatacct gatttggagc aaagtcggtg aaattaccag tggaaaaatc cagattggaa
     1921 ttcccgaagt cagccatgcg gtgattgatg ctgaacttgt gaaacgccgt ccggtgattg
     1981 tgaaaatcct tctggcgccg acgattcccc actgggtctt gattgtcggc aaagaaggtc
     2041 aggaatatct ggtcatggac ccgctcaacg acgacaaacg gctggtcaa
//
LOCUS       DAOQDO010001175         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51575_length_2089_cov_1.892330, whole genome shotgun sequence.
ACCESSION   DAOQDO010001175 DAOQDO010000000
VERSION     DAOQDO010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51575_length_2089_cov_1.892330"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2089
                     /locus_tag="PKZ53_17015"
     CDS             <1..>2089
                     /locus_tag="PKZ53_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42191.1"
                     /translation="EITSSGPGGLLPFADNTDAFEPATGQLQGSRAKVNRDELFNLLR
                     DRWNSDAALRQQLCQRIRENVFKPGIPGFYALAWDNNGTGSNAGGDGKIDLDVNWDPT
                     PNTAENSDPRVGVIDLNEMTVFPNLDPVNQQPKLTAFRTNVGTLTANDFTHLSWPTTT
                     LNPNINNIPSYLKEDPAAANNTFDVERDVLKFLQGLRDVEPGMTNPATAELPRENIVF
                     DWNGDGLIEPPHRPFQGGTPSRNSTITEGIAVGYSVESNICSLIPSTLRVSQTPSTTA
                     DRVTSVPVEVRLVEQNGTVVWVDPANNNAALQSYVGTLMSNNGGQIPFSQADPDPGAG
                     SGFTNNTNSFRIDVPFWTFQRLRTLAKTGTSYNYAITMKVRSAVRIVFQHPLAPRGLT
                     AGSYPTPVGFATQAEVDQRGNQAQIGRHLGLTDAHYAFPGHCYMDFIPTPGDDDIPAG
                     GVPNWLPRVRTKTDTAFPINIWDQREGRHISRQYGFQGIPTSADYHNSDAGTGSKRIL
                     DNQWPDWPNSGTNAADIENNERILNTMAQKLPMPSPIAFERRGMMNIVELDMGNLKRL
                     FRGDFNNFLGNIGGGFTSGQPRYSPIDDSATIWGGNGIIDISDNGLIVYISDRRGDDN
                     NDGLYEFNDVFGENGVLDELDNTVNTVLDPGSDQKGKPGDGIFRFDADVETQPHQLVV
                     DDNSPPRTGESPHG"
BASE COUNT          575 a          486 c          506 g          522 t
ORIGIN      
        1 cgaaattaca agtagtgggc caggtggtct ccttccgttc gccgataata ctgatgcctt
       61 tgaacccgct acgggtcaac ttcagggaag cagagccaag gtcaatcggg atgaattatt
      121 taatttactc cgtgatcgat ggaatagcga tgcagccttg agacaacaac tgtgtcaacg
      181 aattcgtgaa aatgtattta agccaggtat tccaggcttt tatgccctgg cctgggataa
      241 taatggcacg ggatcgaatg ctggtggaga tggcaaaatt gaccttgacg tcaattggga
      301 tccaacccca aacacggctg aaaatagtga tccaagggta ggagtcattg atttaaatga
      361 gatgacagtt tttccaaatt tggatcctgt caatcaacaa cctaaactca ctgcttttcg
      421 gacgaatgtt gggacattga cagcaaatga tttcactcat ctgtcttggc cgacaacaac
      481 cctcaatcca aacattaaca atattccaag ctaccttaaa gaagaccctg cggctgccaa
      541 caatactttt gatgttgagc gcgatgtctt aaaattcttg caaggcctga gggatgtaga
      601 gcctgggatg accaatcctg ctaccgctga attgccacgt gaaaatattg tttttgattg
      661 gaacggagat ggcttaattg aaccacctca ccgcccgttt caaggtggaa ccccttcccg
      721 aaactccact attactgaag gaattgccgt tggatattca gtcgaaagta atatctgctc
      781 cctgataccg agtactttac gagtgtctca aactccttca accacagcag atcgtgtaac
      841 ttcagtccca gtggaagttc gattagttga acaaaatggc accgttgtat gggtagatcc
      901 ggcaaataac aatgccgcac tccaatcata tgtaggcacc ttaatgtcaa ataatggagg
      961 tcaaattcca tttagtcagg cagatccgga cccaggagcc ggatcgggtt tcacaaataa
     1021 tacaaatagc ttcaggattg atgttccttt ttggactttt cagcggttgc gaacattggc
     1081 caagactggt acttcgtata actatgctat aactatgaaa gtccggtctg ctgttcggat
     1141 tgttttccaa caccccctgg cccctcgagg attgactgct ggctcctacc caacccccgt
     1201 tgggtttgca acgcaagccg aagtggatca aagggggaat caagcccaaa ttgggcgtca
     1261 tttgggtctg accgatgccc attatgcctt tccagggcat tgctacatgg attttattcc
     1321 tactccaggc gatgatgata tcccagccgg aggcgtaccc aactggttac cacgcgtgag
     1381 gacgaagaca gacacggcgt ttccgatcaa tatctgggat cagcgtgaag gtcggcacat
     1441 ctcacgtcag tatggattcc aggggattcc aacctcggct gattatcata atagcgatgc
     1501 cggaactgga tccaagcgaa tccttgacaa tcagtggccg gattggccga actccgggac
     1561 gaatgccgct gacattgaaa ataatgagcg gatcctcaat accatggccc agaagctccc
     1621 gatgccgagc ccgattgctt ttgaacgtcg ggggatgatg aacattgttg agctggatat
     1681 ggggaacctc aagagactct ttagaggtga ttttaataac ttcctgggga atatcggggg
     1741 aggctttacc agtggccaac cacgatactc accaattgat gattcagcga cgatttgggg
     1801 tggaaacgga atcattgata tctcagacaa cgggttgatt gtctatattt ccgaccgacg
     1861 cggagatgac aataacgacg gactctatga attcaatgac gtgtttggtg aaaacggagt
     1921 gcttgatgaa ctggataaca cggtgaatac cgtgcttgac ccaggttcag atcaaaaagg
     1981 taaaccagga gacgggatat tcaggtttga tgcagatgtc gaaacccaac cacaccaact
     2041 cgtggtggat gataattctc cgcctcgaac cggtgaatct ccacacggc
//
LOCUS       DAOQDO010001176         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51611_length_2088_cov_2.515002, whole genome shotgun sequence.
ACCESSION   DAOQDO010001176 DAOQDO010000000
VERSION     DAOQDO010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51611_length_2088_cov_2.515002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..831)
                     /locus_tag="PKZ53_17020"
     CDS             complement(22..831)
                     /locus_tag="PKZ53_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ42192.1"
                     /translation="MEENLNDSAMREQIIWKGKFTLWTGGGGHVGYSSDTKIHAHHAH
                     QLCFALDEPFRLRSEPSQDWIEYSAAFVASDVPHQFDGRGNRVVLIYLDPESEDARRI
                     LNTFTGMAIQPLPTQNLSSLLLKLSSDPLMPISSIPFFKGMITVLGGETDVPGTMDQR
                     VIQVIRFLNSFDEEIPTLHSLALKVSLSPSRLGHLFRAQTGLPLRQYRLWLRLQKALE
                     ALTQTHSLTVAAHMASFADSAHLSRTFRRMFGIAPSELKVESNQGRCIPIK"
     gene            894..1763
                     /locus_tag="PKZ53_17025"
     CDS             894..1763
                     /locus_tag="PKZ53_17025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ42193.1"
                     /translation="MLKSILSFFIYPTLVLGGGAVIIWGLGAGLPSAQVVVPVWLISG
                     ICVFCLERFMPYEPDWINNHNDAITDIVHYLVNFAMKQGSLMGYALLVEKLSIVALWP
                     GKWPFGLQVILCLAIIDLFLYLIHRWSHHNDFLWRFHAIHHSSKRLYWVNGEKRHPIH
                     QLLEGVPGVTLVLLVGAPHLVVVSALSILAINMMLQHGNIAYRAGWLRFLFCVAELHR
                     WHHQKEADKSRVNFGAFFALWDLLFKTYRSHSGHLTSQDVGIEEEPDFPTTYFEQLGW
                     PWKRRSGLSENLN"
     gene            complement(1777..>2088)
                     /locus_tag="PKZ53_17030"
     CDS             complement(1777..>2088)
                     /locus_tag="PKZ53_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ42194.1"
                     /translation="EEGEGFTRQMIIRGSLERLVPVMMTALVAALSLIPLALGAGEQG
                     KEILHPVAVVVLGGLITSTLLDQVVTPALFFRFGRVSAEKILGNHAEDLNLASNMTMS
                     T"
BASE COUNT          534 a          458 c          496 g          600 t
ORIGIN      
        1 cccctaacca cttcatgcct tttactttat tggaatacac cttccctgat ttgattcaac
       61 cttcaattca gatggagcaa tgccgaacat gcgccggaac gttcggctga gatgagcaga
      121 atcagcgaaa ctggccatat gtgctgccac ggtaagagaa tgagtttggg tcaaagcttc
      181 aagggccttt tgtaatcgga gccataagcg atactgacga agtggtaatc ctgtttgtgc
      241 tcgaaacaaa tgtcccaggc gactgggcga taggcttact tttagggcaa ggctatgcaa
      301 tgtcggaatc tcttcatcaa atgaattcaa aaatctgatg acctgaataa cccgctgatc
      361 cattgtacct gggacgtcgg tttcgccgcc aagcaccgtt atcatccctt tgaagaatgg
      421 gattgagctg attggcatca aggggtcaga actcaacttc aataaaagcg atgatagatt
      481 ctgtgttgga agcggttgaa ttgccatacc agtgaatgtg ttcaaaattc gcctggcatc
      541 ttcagattca ggatcaaggt atatcaaaac aacacgattt cctctgccat caaactgatg
      601 cggcacatct gaagcgacga acgcggctga atattcgatc caatcttgtg atggctcgga
      661 tcgaagcctg aatggctcat caagcgcaaa acacaactgg tgagcgtgat gagcatgaat
      721 ttttgtgtcg gaagagtatc caacatgtcc tcctcctcca gtccaaagtg taaacttacc
      781 tttccagatg atttgttccc gcatagctga atcgttcaag ttttcttcca taaggtaatg
      841 atagcctggt tgcagtttaa attcacttat caacattggg gggaagcatg gtattgctaa
      901 aatccatttt gtcatttttc atttacccaa cactggttct tggcggcgga gcggttatta
      961 tttggggact tggggctggg ctcccatcag cacaggtcgt ggtgccggtt tggctgattt
     1021 ctggtatatg tgttttctgc ctcgaacgat tcatgcccta tgaacccgat tggattaaca
     1081 atcacaatga tgcgataaca gacattgttc actatttagt gaactttgcg atgaaacagg
     1141 gcagtctgat ggggtatgcc cttttggttg aaaaactatc aatcgttgca ctatggcccg
     1201 gcaaatggcc atttggactc caggttattt tatgtttggc gatcattgat ctgtttttat
     1261 acttgataca ccggtggagt catcacaacg attttctttg gagatttcat gctatccatc
     1321 acagctcaaa acgattgtat tgggttaatg gtgaaaaacg tcatccaatt catcaacttc
     1381 tggaaggagt acctggggtg acgcttgttc ttttggttgg ggcgccacat ctggttgtgg
     1441 tcagtgccct gtcaattctt gcaattaaca tgatgttgca gcatggaaac attgcatacc
     1501 gtgctgggtg gttacgattc ttgttttgtg tcgctgaact tcatcgctgg catcatcaaa
     1561 aggaagctga caagagtcgg gttaattttg gggctttttt tgccttatgg gacttgcttt
     1621 tcaaaactta tcgaagtcat tctggtcatc ttacaagcca ggacgttgga attgaggagg
     1681 aaccggactt tccaaccacc tactttgagc aacttggttg gccgtggaaa agaaggtccg
     1741 gactttcaga aaatcttaac tgatagattt tcagtttcag gtactcattg tcatatttga
     1801 agccaggttt aagtcttcgg cgtgattgcc cagaattttt tccgcgctga ctcgaccaaa
     1861 acggaaaaac aatgctggtg ttacaacctg atcaaggagc gttgaggtga tgagtcctcc
     1921 aaggacgacc actgcgacgg ggtggaggat ttcttttcct tgttctccag ccccaagcgc
     1981 cagaggaatc agcgaaagag ccgccaccaa agccgtcatc ataactggaa ccagacgttc
     2041 cagtgaacca cggataatca tctggcgcgt aaagccttcg ccttcttc
//
LOCUS       DAOQDO010001177         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51622_length_2088_cov_2.240531, whole genome shotgun sequence.
ACCESSION   DAOQDO010001177 DAOQDO010000000
VERSION     DAOQDO010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51622_length_2088_cov_2.240531"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKZ53_17035"
     CDS             <1..322
                     /locus_tag="PKZ53_17035"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ42195.1"
                     /translation="LTLIAILTKLVGCGLAALPGNGVRTGLQVGMGMVPRGEVGIIVA
                     QIGLSLGVLSASLYGIVVFMAVMTTLAAPPFLTRLFASEIRSEVAQVDAQVIEEDQIF
                     SDIQ"
     gene            376..2019
                     /locus_tag="PKZ53_17040"
     CDS             376..2019
                     /locus_tag="PKZ53_17040"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNJ42196.1"
                     /translation="MSPHSFDQVYHFSVAFFEISEAERREVYAEIRDSYQMRPSYLIM
                     LVLACLVAILGLLANSPAVVIGAMLISPLMSPFLSAGLALAIGDWELGKAAMRTILVS
                     VLAAIFISALAVAVSPLKEPTAEIVARTNPNLLDLGIAFFSGCAGTYTLVSRKGTTTI
                     PGVAIATAVMPPLCVVGFGVYYLDPRIIFGALSLFVTNLAAIIISAAFLFYLASFRIS
                     EFKDEKPHWSISTRLTISFVVLGLLAIPLASALVNAAETLRLRKKVEAALTEYLKHEE
                     ARARLVGGWSVMRIKDRGLVVEATVRTLSYFTKSEIDAWTRTLEQTLGEPVDLQLDQI
                     QVRKGGLEPAVTFKPPEPVVLPVTDPTYFSRLMNEYKPVVEFTVKTIDAELDQFLLVT
                     DHQGVIRIEIRARVDRVPSEDICATSIRGAFAQLEALHLPRQPQPEINFYFLPKQAEK
                     LPVFLKAGKFTLRPEPIKLIADRLKGFRSQVSPPGLQIKFDQSLQLDESIKTDLQRQI
                     MKALAVSQTSDEPSGDKIQSLGQSSQIEILVFASDETNF"
BASE COUNT          490 a          471 c          537 g          590 t
ORIGIN      
        1 cctgacgctg attgcaattt tgaccaagct ggttgggtgt gggctggcag cattgcctgg
       61 aaatggagtg cgaaccggtc tgcaggttgg catgggaatg gttccccgtg gcgaagttgg
      121 aatcatcgtg gctcaaattg gtctcagtct tggggttttg tcggcttcgc tgtatgggat
      181 tgtggtgttt atggccgtga tgaccacgct ggcggcgccg ccgtttctga cccggttgtt
      241 tgcttcagaa attcgatctg aggtggctca agtggatgca caggtcattg aagaggatca
      301 gattttttcc gatattcaat aacctacagg aaagtcggtt ctgaatagga tttcatcttt
      361 ggagtaacaa ttggtatgtc accgcattca ttcgatcagg tttaccattt ttcagtcgcg
      421 ttctttgaga tttcagaagc agaacgtcgt gaagtgtacg ccgaaatcag ggatagctac
      481 cagatgcgac cttcctacct gataatgctg gttttggcgt gtctggtggc aatcctggga
      541 ttactggcca acagtccggc agttgtcatt ggcgccatgc ttatttctcc gctgatgagt
      601 ccatttctgt cggctggatt agccctggct attggtgatt gggagctggg aaaagccgcc
      661 atgcgcacca ttttggtgag tgttctggct gcaattttca tctcagctct tgccgtagcg
      721 gtgtctcctc tcaaagaacc aaccgcagag attgtcgcac gaacaaatcc caatctactg
      781 gatttaggaa ttgcgttctt ctcagggtgt gcgggaacgt acactctggt ttcgcgcaaa
      841 ggaaccacca cgattccagg tgtggcgatt gcaacggctg tgatgccgcc actgtgtgtc
      901 gttggatttg gggtatatta cctggatccc cggattatct ttggtgcctt gtcgcttttt
      961 gtgaccaatc ttgcagccat tattatcagt gctgctttcc tgttttatct ggcatcgttt
     1021 cggatttcgg aatttaaaga cgaaaagcca cactggtcaa tttcaactcg attaaccatc
     1081 tcatttgtag ttctggggct actggctatc ccgctggctt cagctttggt caatgcggct
     1141 gaaactttac gcttgcggaa aaaagtagaa gctgcgttga cggaatatct caaacacgaa
     1201 gaagctcgtg cccggctggt tggtggctgg tcggttatgc ggataaaaga ccgtggcctc
     1261 gtggttgaag ccactgttcg aaccctttct tattttacaa aatcagagat tgacgcctgg
     1321 acaagaacgc ttgaacaaac tcttggtgag ccggttgatc tccaactcga tcagattcag
     1381 gtacggaaag gaggattgga accggcagtg actttcaaac cgcccgaacc agtcgttctt
     1441 ccagtaacgg atccaaccta cttttcccgg ctgatgaatg agtacaaacc cgttgtcgaa
     1501 ttcacggtca aaacgataga tgccgagttg gatcagtttt tattagtcac ggatcatcag
     1561 ggggtgatcc gaattgagat cagggcacgg gttgatcgtg ttccgtctga agatatttgc
     1621 gctaccagta ttcgaggcgc attcgcccaa cttgaagcat tgcatctccc gcgacaacca
     1681 caaccagaga tcaattttta tttcttaccg aagcaagcgg aaaaacttcc ggtttttctc
     1741 aaggctggga aattcacact tcgtcctgag ccgatcaaat tgattgccga cagattgaaa
     1801 ggatttcggt cacaggtttc accgccaggt cttcagatca aatttgacca atcccttcaa
     1861 ctggatgagt caattaaaac cgatttgcag cgacaaatta tgaaagcgct cgcggtatca
     1921 caaacatcgg atgagccttc gggtgacaag attcagagtt taggccaatc ctctcagatc
     1981 gagatcctgg tttttgcttc tgatgaaacg aatttttaac atggggtaaa tttggtgttc
     2041 aaacggttga aacccaaccg tgttagaact gaagatgcat cggtaaca
//
LOCUS       DAOQDO010001178         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51836_length_2083_cov_3.327909, whole genome shotgun sequence.
ACCESSION   DAOQDO010001178 DAOQDO010000000
VERSION     DAOQDO010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51836_length_2083_cov_3.327909"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKZ53_17045"
     CDS             complement(<1..859)
                     /locus_tag="PKZ53_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42197.1"
                     /translation="MVNPLAKASTVMAAPDVRTVVQELIHQSHDLQGAPQIRLLESAL
                     RLAEVAQDDGLSRTIHFELIRALVQTRQIPKLLASVAWLKARSHEPGDTKLTSVLYPF
                     VIQQLPQFHTLPLTDIQTSLSELDRDLRLTRNGEFRSVYVQWAVNWDLGQIDRAIDLY
                     QEFQKYSLQAGGEWPCEACALLARVRFQIFLGNDQEAVEMAGPLRQQRVTCERDFQVK
                     VLTTLMLPLLRLGHSAEAVQYHLLTVKRLHAQVERILLVQPHIAFLCLTGNNAAAIKL
                     FEHYLPEVFN"
     gene            complement(874..>2083)
                     /locus_tag="PKZ53_17050"
     CDS             complement(874..>2083)
                     /locus_tag="PKZ53_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HSP90 family protein"
                     /protein_id="HNJ42198.1"
                     /translation="NPEPPPWKITTANYHEYQRVSLRYGKRALGFDFIDCFPIQSQIG
                     DVEGIAYVLPVALHPGTKPRHRAYLKNMLLSEECDRLLPDWAFFVTCVINANDLRPTA
                     SRESFYEDEKFEAVREELGNSLKSFLLNLIARDPQRLQKILAIHHHSIKYLALFDDEL
                     FQVFVDLFPFQTTHGRITLGEYRRANPVIRYTAELDQFRQLLPIARSQNLCVINAGYA
                     YDAQLLEKLGDLDPDALMEPITGLSLTSQFSGLNPDTDLITFQFLKAAEQVLKPFHCF
                     VTLKKFDPPTVPALYSLTESGAFLRSVERTKEQSDELLASILTSLSQESISTEPQAQI
                     CFNYNNPLIQKLCNLGDSNLLSLSIRFLYLQSLLMGHHPLTEKELALLNEGLLGFVQW
                     GLETQSEFSA"
BASE COUNT          543 a          489 c          552 g          499 t
ORIGIN      
        1 aattgaaaac ttcaggaagg tagtgctcaa aaagcttgat ggcggctgca ttgtttccgg
       61 tcaggcagag aaaggcaatg tggggctgaa cgagcaggat gcgttccact tgtgcgtgca
      121 ggcgttttac ggtgagtaag tgatactgaa cggcctcggc actatgccca agtctcaaaa
      181 gcggtaacat cagtgttgtc aggaccttga cctggaaatc ccgttcacag gtgacacgct
      241 gctgccgcag gggaccagcc atctcaacgg cttcctgatc atttcccaaa aaaatctgga
      301 atcttaccct ggctaacagg gcgcaggctt cacatggcca ttctcctccg gcctgcaatg
      361 aatatttttg aaattcctga tagagatcaa tggcccggtc aatctgaccc aggtcccaat
      421 tcactgccca ctggacatag actgatctga attcaccatt tcgagtcagt cgcaaatctc
      481 gatccagttc tgaaagtgat gtttgaatgt ctgtgagcgg aagagtgtga aattgtggaa
      541 gttgctggat cacaaaagga tacagcacac tggtcaattt ggtgtcaccg ggctcgtggc
      601 ttcgggcttt taaccaggca acactggcca gcaacttggg aatctgccgg gtctgaacca
      661 gggcccggat caattcaaaa tggatggttc gtgacaatcc atcgtcctgt gccacttctg
      721 ccagccggag cgctgattcg agcaggcgaa tttggggcgc gccttgaaga tcgtgagact
      781 ggtgaatgag ttcctgaacg acagttcgaa cgtctggagc cgccataacc gttgatgctt
      841 tcgccagtgg attgaccatc aggggagttc tggtcaggca ctaaattcac tctgtgtttc
      901 cagcccccac tggacaaacc caagcaatcc ttcatttaaa agagcgagtt ccttttccgt
      961 caatggatga tgtcccatca aaagtgattg aaggtaaaga aaccggattg acagcgataa
     1021 caggtttgaa tcaccaaggt tacacagctt ctggatcaat gggttgttgt aattaaagca
     1081 aatttgggcc tgcggttctg ttgaaatgga ttcctgggac agactggtga gaattgaggc
     1141 cagtaattcg tcagactgct ctttggttcg ttcaaccgat ctcaaaaagg cacctgattc
     1201 ggtcaagcta tacagtgccg gcaccgtcgg cgggtcaaat ttctttaatg tcacaaagca
     1261 gtgaaaaggc ttgagcacct gctctgcggc ttttaaaaac tggaaagtga tcagatcggt
     1321 atccggattg agccctgaaa attgtgaggt cagcgagagc ccggtgatgg gttccatcag
     1381 ggcgtcaggg tccagatccc caagtttttc aagcaattga gcatcataag catacccggc
     1441 gttgatcacg cacaggtttt gcgacctggc aatcggaagc aactggcgaa attgatcaag
     1501 ctctgctgtg tagcgaataa ccggattggc gcgccgatat tctccgagcg tgattcgacc
     1561 atgggttgtt tgaaagggga acaaatcaac aaagacctgg aacaactcat catcaaataa
     1621 agccagatat ttaattgaat gatgatggat ggccaggatt ttttgaagcc gctgagggtc
     1681 acgggcaatc agattgagaa gaaacgattt gagtgaattg cccagttctt cgcgcaccgc
     1741 ttcaaacttt tcatcttcgt aaaaagactc acgcgaggcg gtgggccgca ggtcattggc
     1801 gttgatgaca caggtcacaa aaaacgccca gtccgggaga aggcgatcac actcctctga
     1861 cagcagcatg tttttcaaat aggcccggtg cctgggtttg gtaccggggt gcaacgcaac
     1921 cggaagcaca taggcaatcc cttcgacgtc gccaatctga ctttgaatcg gaaagcaatc
     1981 aataaaatca aacccgagtg ctcgcttgcc atatcggaga gaaactcgct gatactcgtg
     2041 ataattcgcc gtcgtgattt tccagggggg cggctcgggg ttg
//
LOCUS       DAOQDO010001179         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51951_length_2081_cov_2.766535, whole genome shotgun sequence.
ACCESSION   DAOQDO010001179 DAOQDO010000000
VERSION     DAOQDO010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51951_length_2081_cov_2.766535"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKZ53_17055"
     CDS             complement(<1..237)
                     /locus_tag="PKZ53_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42199.1"
                     /translation="MTLCHKAFAFHWVGFASQLKPLLEQALVTSDPMPLQMFLLANQT
                     DCRDPYEGNPLDETSWKALENLGVQTWADFSLTVF"
     gene            533..1927
                     /locus_tag="PKZ53_17060"
     CDS             533..1927
                     /locus_tag="PKZ53_17060"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ42200.1"
                     /translation="MKSSPPPMSQTKDTIRVILIGAGNGGKSLLEIFSDDPMIAVVGV
                     ADKNEQASGFRYAQELKLPVTTNYRQLLATTPADIIINVTGDSEISRELYALKPERTE
                     LIGGTTARLMCELIWTKKQSQALEEKYQLALRELEAYAETEFIIGNSPQMKEVSRLIT
                     QVAPTPTTVLISGESGTGKELVARAIHRHSLRSSRVLVTLNCTALPPTLLESELFGYK
                     RGAFTGAFKDQIGLFEKADGGTMFLDEIGDMSLEVQAKLLRTLQTGEIRAVGNVKSKV
                     VSVRIIAATNRDLKKAIEEGRFREDLYYRINTFTIMLPSLRERKADIPLLAEYFLLRA
                     RAKVNKRVEVFSPKALELLRQYDWPGNLRELENIIERAVVLTNSTMVEAEHLPIHVQE
                     KNPSLILDAGFMEAKAAAIDRYEKEVLLTFLTQAQGNVSVAAKMAKLPRRTFHRLLSK
                     HQVMAASFKGKKSK"
BASE COUNT          564 a          531 c          484 g          502 t
ORIGIN      
        1 aaagacagtt aaggaaaagt cagcccaggt ttggaccccc agattttcaa gcgctttcca
       61 actggtttca tcgagtgggt ttccctcata tggatcccgg cagtcagttt gatttgccag
      121 aagaaacatc tgcaatggca ttggatccga tgttaccagg gcctgttcga gcaatggctt
      181 gagctggctg gcaaatccaa cccaatgaaa ggcaaacgct ttatggcaca gtgtcataaa
      241 ccacctcagc cacgtcaaga taaccttgac ttgaaaaaat gaaaattctg ataaactcaa
      301 ccttgagagt tataccatcc catctaaaaa atacaatttc ttcaatcagt tagcggaaag
      361 ctggttaaac ttgataatcc ctcatttgga cgaagtccga cttttgcgca acggagttca
      421 cactctctga acccaaccga aaaggcaaaa acccatgctc ccgcttcgct cattttcctg
      481 atacactcgg cgatgcccgg taatggatca cccccgatct tctcaattcc ccatgaaatc
      541 ttctcctcct ccaatgtcac aaaccaaaga cacaattcga gtcattttga ttggcgctgg
      601 taatggcgga aaatcgctgc tggaaatttt ttcggatgac ccgatgattg cggtcgtcgg
      661 agtggcggat aaaaatgagc aggccagcgg atttcgctat gcccaggaat tgaaactccc
      721 agtcacaacc aactatcgcc agctcctggc gaccacaccg gctgatatca ttatcaatgt
      781 caccggagat tccgaaatca gtcgtgaact ctatgcttta aagccagagc gcaccgaact
      841 gattggcggg acaaccgccc ggttgatgtg tgagttgatt tggaccaaaa aacaatccca
      901 ggccctggag gaaaaatacc agcttgcatt gcgtgaactg gaagcctatg ccgaaacaga
      961 atttatcatc ggtaactccc cacaaatgaa agaagtttcc cgcctgatta cccaggtggc
     1021 gccgacgccg acgacggttc tgattagcgg ggaaagcggt acggggaagg aactggttgc
     1081 ccgggctatc caccggcaca gtttgcggtc gtccagggtg ctggtcacgc tcaactgtac
     1141 ggcccttccg ccaaccctgc tcgaaagtga attgtttggc tacaaacgcg gcgcctttac
     1201 cggtgccttt aaagatcaga tcggactatt tgaaaaagcg gatggcggca ccatgttcct
     1261 ggatgaaatt ggcgatatgt cgctcgaagt ccaggccaaa ttgttgcgga ccctccaaac
     1321 cggcgaaatt cgcgccgtgg gcaatgtcaa atcaaaggtt gtctctgtca gaattattgc
     1381 cgccaccaat cgcgacctga aaaaagccat tgaagaaggt cggtttcgcg aagatctcta
     1441 ttatcgaatc aatactttta cgatcatgct tccttcgctt cgtgaacgga aagcagatat
     1501 tccactcctg gctgaatatt ttctcttgcg ggcccgcgcc aaagtcaata agcgggttga
     1561 ggtcttttca cccaaggcac tcgaactcct gcgccagtac gactggccag gaaatttacg
     1621 tgaattggaa aatattatcg aacgggcggt ggtgttgacc aattccacca tggttgaagc
     1681 cgaacacctt ccgattcacg tccaggaaaa aaatccgtcg ctgatccttg acgcagggtt
     1741 tatggaagcc aaagccgccg ccattgaccg gtatgaaaaa gaggtcctgc tcactttttt
     1801 gacccaggcc cagggcaatg tttcagtggc cgccaaaatg gccaaacttc cccggcggac
     1861 cttccatcgg ttattgagca agcaccaggt tatggccgca agttttaaag gaaaaaaatc
     1921 aaaatgagac atttttgact cagatgagac atttttagct cactcctgat atatccctgt
     1981 caactgattg atttgattgg ttctttgtgc aggttttgct caaagaagga gagaatctga
     2041 gacatttctg gcccactttt ggaaaatctg cctggcaacc a
//
LOCUS       DAOQDO010001180         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_51967_length_2081_cov_2.254195, whole genome shotgun sequence.
ACCESSION   DAOQDO010001180 DAOQDO010000000
VERSION     DAOQDO010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51967_length_2081_cov_2.254195"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKZ53_17065"
     CDS             <1..624
                     /locus_tag="PKZ53_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNJ42201.1"
                     /translation="EYGKALKTFARRYPFAGYGDRFDHWARDDSYAPYNSCGNGSAMR
                     VSGVGYAFDSLEDVVTEARETARVTHNHPEGIKGAQAVAACIFLARTGVEKSAIKQVI
                     ATCFQYDLDQKLDEIRPTYFFDVTCQGSVPQSIVAFLESDNFESAIRNAISLGGDSDT
                     MACIAGSIAQAYYRHIPPEIIEFVRRKLPADLLEVVDAFNERFEVLY"
     gene            634..1284
                     /locus_tag="PKZ53_17070"
     CDS             634..1284
                     /locus_tag="PKZ53_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42202.1"
                     /translation="MAFLLKAEKHPTRCEICHQSDQFDPVANHCTRCQGVVERHRPAS
                     QRVVWKIVAGVGLALIVIVSLGAGAITLWVSWIRSLEKQPPPIVKEIDPIRPLTVDLL
                     TEQINKNLTPGTSDVPQVLNFLETRTLQHDPLKPVTADDVFCTENRSQDLKESHYLII
                     EKNIKHKITAWTPITGRNYLHHWIIVMRFYFDENQKLVCSSVCHWPVDPGYPRPTS"
     gene            1500..>2081
                     /locus_tag="PKZ53_17075"
     CDS             1500..>2081
                     /locus_tag="PKZ53_17075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ42203.1"
                     /translation="MKHIKFLLSACLCLWFWIVPATAQVTSSAITGTVRDESQHPVRQ
                     TTLTVKNLETGLTRKTISADDGSFSIFGLPPGRYLVQAEKSGFTEARLDELVTSVAEE
                     KNIHLILKVIPVQEVVTITSEPSLIEVSTSTISGLVTDRQVPALPLNGRDLTQLILLQ
                     PGVVNSRASVQSGSTGQGSRFSVSGARPTQNVFT"
BASE COUNT          475 a          519 c          524 g          563 t
ORIGIN      
        1 gagtatggca aagccctcaa gacgtttgcc cgccgctatc cctttgccgg gtatggcgac
       61 cggtttgacc actgggcgcg ggatgattcc tacgctccgt ataacagttg tggcaacggg
      121 tcagccatgc gggtcagcgg ggtcggctat gcctttgatt cgcttgaaga tgtggttacc
      181 gaagcccgtg aaaccgcccg cgtgacccat aatcatcccg aaggcattaa aggcgcgcaa
      241 gccgtggccg cctgcatttt tctggcgcga accggcgttg aaaaatcagc catcaaacag
      301 gtcattgcga cctgctttca atatgatctg gatcaaaaac tggatgagat tcggccaact
      361 tattttttcg atgtgacctg tcaggggtcg gtgccgcaat ccatcgttgc ctttttggag
      421 tcagacaatt tcgaaagtgc cattcgcaat gcaatttcgc tgggtgggga cagtgatacg
      481 atggcctgta ttgccggcag cattgctcaa gcctattacc ggcatattcc tcctgaaatc
      541 attgagtttg tccggagaaa acttccggct gatttgctgg aagtcgtgga cgctttcaac
      601 gagcggtttg aggttctgta ttaaggtcac gatatggcat ttctgttgaa agctgaaaaa
      661 catccaaccc ggtgcgaaat ttgccaccaa tcagatcaat ttgatccggt tgcgaatcac
      721 tgcacgcgat gtcagggggt ggttgaacgt caccgaccgg cttcacaaag ggtggtgtgg
      781 aaaatagttg ccggggttgg attggcgctg attgtaattg tgtcgctggg cgccggtgcg
      841 atcacgttgt gggtttcgtg gattcggtcg cttgaaaaac agccacctcc catcgtgaaa
      901 gaaattgacc cgattcgtcc cctgacggtt gatcttctga cagaacagat caataaaaat
      961 ctgacacctg gcacatcgga cgtccctcaa gtactgaatt tcttagaaac tcgaaccctc
     1021 cagcacgatc cgctcaagcc agttaccgct gatgatgttt tttgtaccga aaatcgaagc
     1081 caggacctca aagagtcgca ctatctgatt attgagaaaa atatcaaaca taaaatcacc
     1141 gcctggacgc cgattacagg ccgcaattac ctccatcact ggattatcgt catgcggttt
     1201 tactttgatg agaatcaaaa actggtttgt tcgagtgttt gccattggcc ggtggatcca
     1261 ggttacccgc gaccaacctc ctgattggcg tgttttcaaa gctatcgttg ttcagtaaat
     1321 atttccctgg tctatatttg aatttatttt ctgtgtgttc tgtgtattcc gtggtttctt
     1381 tttctgaaaa tctcatttcc atctggcatt tccaccttct ttccactaga ttgcgcagcc
     1441 aatacctcaa aaaattctct cgatgcccct cctgatgcca gtcaggagct attttctcta
     1501 tgaagcacat caaatttctg ctcagtgcat gtctttgcct gtggttttgg attgtgccgg
     1561 ctacagccca ggttaccagt tcggccatta ccggcaccgt tcgggatgag tcacaacacc
     1621 cggtgcgtca gactacgtta acggtgaaaa atctggaaac tggcctgacc cgcaaaacaa
     1681 tcagtgctga tgatggatcg ttttcgatct ttggtttgcc tccagggagg tatctggttc
     1741 aggccgagaa atccggtttt accgaagcgc ggctggatga actggtcact tcggtggctg
     1801 aagagaagaa tattcatttg attttaaagg tgataccggt tcaggaagtg gtcactatca
     1861 ccagtgagcc atcgctgatc gaagtgtcca cctcaaccat cagcggtctg gtcactgatc
     1921 gccaggtgcc ggccctcccg ctcaatggtc gcgatttgac ccagttgatt ttgcttcaac
     1981 ccggagtcgt caacagtcga gccagcgtgc agtcaggcag caccggtcag gggtcgcggt
     2041 tttcagtttc tggcgctcgt ccaacccaga atgtgtttac c
//
LOCUS       DAOQDO010001181         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52036_length_2079_cov_3.248518, whole genome shotgun sequence.
ACCESSION   DAOQDO010001181 DAOQDO010000000
VERSION     DAOQDO010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52036_length_2079_cov_3.248518"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2079)
                     /locus_tag="PKZ53_17080"
     CDS             complement(<1..>2079)
                     /locus_tag="PKZ53_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42204.1"
                     /translation="PAARGAIGDDRLFAICEDRRRTLWVGMAGPKGLLTLNRRTQRFE
                     RSSLIPSNAIIYSLYEDSKENLWVGTNGGLYQIDSNRQQSRLYQQELGFQPGQYMDVQ
                     SVMEDRQHWLWVGLDGNLIRYNPETGVSVHYDQQLKINQTGILVGCIREDSKGQIWIA
                     TKGAGIFCYDPARDRFEQFTTHDGLPHNVTYAVLEDLQGKYWVSTDNGIARFDPVHRT
                     FVLFGPEHGLQGKEFNRGAFCRTRSGVMFFGGTNGLNQFTPDSIQPTSSIPPVVVSQI
                     LVNGIPVPVTESVALGDKQNTVGFEFAVLDFNAPELNQYAIQLEGGNQDWVGVTGRPQ
                     VVYGNLLPGTYFFRVRGANSSGVWNTQGAVIRLVISPPWWRTSWAYAGYLVLLGGSLS
                     FFGWLVRFRAATRIQLREAMVLARAATAEREVERARAEAAEIQKKAIETENLQRAEAE
                     AVIRSKNEELGRMVDQLKDSERQAVEAKEEAVQASRAKSEFLANMSHEIRTPMNAILG
                     MAQLLSDSTLTSHQQECAQIIQSSTESLLTIINDILDFSKIEAGVIELEELPFDLAGC
                     LEEVVKIFSRPAKAKGIELRMRIDPELPSQVCGDPGRIRQILLNLIGNAVKFTHHGWI
                     ELTVHVDRAPDALPDSIRFEVQDTGIGIPADRRDRLFKSFSQVDASTTRKYGGTGLGL
                     AISSRLVALM"
BASE COUNT          494 a          599 c          482 g          504 t
ORIGIN      
        1 ccatcaaggc cacgagccgg ctactgatcg ccagtcccag cccggtgcca ccatattttc
       61 gggtcgtcga agcatccacc tgactaaatg atttaaataa ccggtcccgc cgatcagccg
      121 gaatcccaat gccagtatcc tggacttcaa atcggatcga gtccggcaac gcatcaggtg
      181 ctcgatcaac gtgcaccgtc agctcaatcc acccgtgatg cgtaaacttc accgcatttc
      241 caatgagatt gagcaggatc tggcgaattc ggccaggatc gccacatacc tgagacggaa
      301 gctcaggatc aattcgcatg cgtaattcaa tccctttcgc cttcgccggt cgggaaaaaa
      361 tcttgaccac ctcctccagg cacccggcta aatcaaatgg cagctcttcg agttcgatca
      421 cccctgcttc aatttttgag aaatccagaa tgtcattgat gatggttaac aatgactcgg
      481 tacttgattg gataatttgc gcgcattcct gctggtggga tgtcagggtt gaatcagaca
      541 aaagctgggc catgcccaga attgcattca tcggggtccg gatttcatgg ctcatattgg
      601 ccagaaactc agacttggcc cggctggctt gaaccgcttc ctctttggct tcgaccgcct
      661 gacgttctga gtcttttaac tggtctacca tccggcctaa ctcttcattt ttactgcgaa
      721 tcacggcttc ggcctcggct cgctggagat tttcggtttc gatggccttt ttttgaattt
      781 cagccgcttc agctcgcgcc cgttcaactt cccgttcggc ggttgccgcg cgggccagaa
      841 ccatggcttc ccggagttga atccgggtgg ccgcccggaa ccgaacgagc cagccaaaga
      901 acgaaaggct tccacccagc aaaaccagat agccagcata ggcccacgaa gttcgccacc
      961 agggcggcga aatcaccagc cgaatcacag ccccctgtgt attccagacc ccactgctgt
     1021 tggcgcctct gactcgaaaa aagtatgttc caggcagcag gttcccatat accacctgag
     1081 gccgcccggt gaccccaacc cagtcctgat tgcccccctc cagttgaatt gcatactggt
     1141 ttaactctgg tgcattgaag tccagaactg caaattcgaa tccaaccgtg ttttgtttat
     1201 ctccaagtgc cactgactca gtgactggga ccgggattcc attcaccaga atctgactca
     1261 ccaccactgg cggaattgaa gaagtgggct ggattgaatc tggagtgaat tgattgagcc
     1321 cattggtgcc gccaaagaac atcacccctg accgtgttcg gcagaaagcg ccccggttaa
     1381 attccttgcc ctgcaaacca tgttccggcc caaataagac aaatgtccgg tgaaccgggt
     1441 caaagcgggc aataccattg tcggtactca cccaatactt cccttgcagg tcttctaaca
     1501 ccgcatacgt tacattatgg ggcaatccat cgtgggtcgt gaactgttca aatcgatccc
     1561 gagctggatc ataacaaaat ataccggcgc ctttggtagc gatccagatt tgtccttttg
     1621 aatcttcgcg aatacaccca acgagaattc cggtttgatt gattttgagt tgctgatcat
     1681 agtggacgct gacgccagtt tccggattgt agcgaattaa gttgccatcg agcccgaccc
     1741 agagccaatg ctgacgatcc tccatgactg actgcacatc catatactgg cctggttgaa
     1801 atcctaactc ttgctggtag aggcgtgatt gctggcgatt tgagtcaatt tgatacaatc
     1861 ctccatttgt acccacccag aggttttctt ttgaatcttc atacaaacta taaataatgg
     1921 cattggatgg gatgagggaa gaccgctcaa accgctgtgt ccgacgattc aaagtcagca
     1981 acccctttgg tccagccatt ccgacccaca gtgttctccg acgatcttca caaatggcaa
     2041 ataatcggtc atcaccaatc gcccccctgg cggctggct
//
LOCUS       DAOQDO010001182         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52037_length_2079_cov_3.220850, whole genome shotgun sequence.
ACCESSION   DAOQDO010001182 DAOQDO010000000
VERSION     DAOQDO010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52037_length_2079_cov_3.220850"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKZ53_17085"
     CDS             complement(<1..1006)
                     /locus_tag="PKZ53_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ42205.1"
                     /translation="MNILSITQVTKSFGLQPVLDKVSLAINLGERVAIIGANGSGKTT
                     LFNMISGEVEPDSGGVSLRRGISVGVLPQEPQFDPTATIHDTLAASLVEIQQVIADYQ
                     AITEQMAATTNETEFLKLQAAHDEIEHRLEHLGGWQYQHRLDTLRGYLDIPDDQKRMG
                     ELSGGERKRVALACAFLRNPDLLVLDEPTNHLDATTISWLEQYLDTYPGSLLLITHDR
                     YFLDNIVDRMVELSGGKAIGYKGGYSDYLMARAEAEAQEARSHAKLLNLLRREEAWLR
                     RGVRARGTKSKHRINSVLELREQARREAELNLSAHYAIQKDLGNTILETRQVTVTIEG
                     R"
     gene            1216..1983
                     /locus_tag="PKZ53_17090"
     CDS             1216..1983
                     /locus_tag="PKZ53_17090"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ42206.1"
                     /translation="MRIIAIANQKGGSAKTTTTINLGAALALQKKRVLLVDMDPQGHL
                     AEGFGIPAESVEKDISLVLEKKAKLGEVIQNLRPKLDLAPSNILLSQTEAVLFTKTRR
                     EDRLKAALQQLGHEYDFVLIDCPPSLGLLTVNCLAAAQEVLITMAAEYYALLGVGLLL
                     NTLEDLREELNPDLKILGIVATRVTRTTHSRDVLSHVEENLTSRARLLQTQVKEQVAL
                     RNCALSGQTIFEFAPDSEAAQSYTALAKEVIRAGKAK"
BASE COUNT          488 a          539 c          563 g          489 t
ORIGIN      
        1 tccggccttc gattgttaca gtgacctgcc gggtttccag gatggtgttg cccagatctt
       61 tttgaatcgc atagtgggcc gagagattga gttcggcttc gcgtcgggcc tgttcgcgga
      121 gttcgagcac actgttgatt cggtgtttgg atttggtgcc acgggcgcgc accccccggc
      181 ggagccaggc ttcttcgcgt cgcaacaggt tgagcaattt ggcgtgtgac cgggcttcct
      241 gggcttcggc ttctgcccgg gccatcaggt aatcagaata gcccccctta tacccaatcg
      301 ctttgccacc agagagttca accatccgat ccacaatgtt atcaaggaaa taccggtcgt
      361 gggtaatcaa caacagtgac cctgggtagg tgtcaaggta ctgttcgagc caggaaatcg
      421 tggtcgcatc aagatggttg gtgggttcgt cgaggaccag caggtccgga ttgcgcagga
      481 aagcacaggc cagcgccacg cgtttgcgtt cgccaccact gagttctccc atccgtttct
      541 ggtcatccgg aatatcaaga tacccgcgca acgtatcgag ccggtgctgg tattgccagc
      601 cgccgaggtg ttctaaccgg tgctcaatct catcatgagc cgcctggagc ttgagaaatt
      661 cggtttcgtt ggtggtggcg gccatttgtt cggtaatcgc ctgatagtcg gcgatgactt
      721 gctggatttc gaccaggctg gcggccaggg tgtcgtggat ggtggcagtt gggtcaaatt
      781 gaggctcctg aggaagcaca ccgacggaaa tgcctcgccg gagcgaaaca ccccctgaat
      841 caggctcaac ttcaccggaa atcatgttaa agagcgtcgt ttttccgctg ccattggccc
      901 cgatgatggc aacccgttca ccgagattga tggccaggga gactttatcg aggacgggtt
      961 gtaacccgaa ggacttggta acttgtgtaa tactgaggat attcattttg gagttggcta
     1021 atttgaaaag gttgcgacaa tatagccatc cattccccaa atatcaaaaa aagatgcctt
     1081 tggagtgtgg ccccttgacg ctccctttgg agtgcggcgg cttgacgccg ctttcaacgt
     1141 acaaaaaagg gagctgattg gaagacgtgt atttctccga aaaccctgaa tcctgaactc
     1201 tcaaaagata tgaaaatgcg catcatcgca attgcaaatc aaaaaggtgg ctcagcgaaa
     1261 accaccacca ccatcaatct tggagccgcg ctggcactcc agaaaaaacg ggtcttgctg
     1321 gttgatatgg atccgcaagg ccatctggct gaaggatttg gcatcccggc agaatcggtc
     1381 gagaaagata tttcactggt cctggaaaaa aaagccaaat tgggcgaggt catccaaaat
     1441 ctgcgcccca aacttgatct ggcaccttca aatattttgc tttcccagac tgaagcggtc
     1501 ttgtttacca aaactcggcg tgaagatcgc ctgaaagccg cgctccagca gttaggccat
     1561 gaatatgatt ttgtgttgat tgattgcccg ccatcgcttg ggttgctcac ggtcaactgt
     1621 ctggcggcgg ctcaggaagt gctgattacc atggccgccg aatattacgc cctgctcggt
     1681 gttggcctcc tgctcaacac gctcgaagac cttcgggaag aactcaaccc ggatctgaaa
     1741 attctgggaa ttgttgccac ccgggtgacc cgaacgaccc attcccgcga tgtgctctca
     1801 cacgtcgaag aaaatctcac ctcacgggcc cggttgctgc aaacccaggt caaagaacag
     1861 gttgccttgc gaaattgtgc gctttcaggg caaacgatct ttgaatttgc tcccgattcc
     1921 gaagctgccc agagttacac agcattggcc aaagaagtca ttcgggcggg aaaagcgaag
     1981 tagcaggcta aaaacaagtc gcgaggagtc agaaagactg agtcgcaaat cgctactcgc
     2041 ttgatacttc gcaacttcac tctgattggc atagccttt
//
LOCUS       DAOQDO010001183         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52038_length_2079_cov_3.186759, whole genome shotgun sequence.
ACCESSION   DAOQDO010001183 DAOQDO010000000
VERSION     DAOQDO010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52038_length_2079_cov_3.186759"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..77)
                     /locus_tag="PKZ53_17095"
     CDS             complement(<1..77)
                     /locus_tag="PKZ53_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNJ42207.1"
                     /translation="MLPNVVIIGRPNVGKSTLFNKLTGTR"
     gene            600..>2079
                     /locus_tag="PKZ53_17100"
     CDS             600..>2079
                     /locus_tag="PKZ53_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNJ42208.1"
                     /translation="MSENLDQNAWGTLRYRDVTGKERVVVLNKAPFTIGRLGDNHLQL
                     DDPSISRHHVELVFDGKTYRMRDKQSTSGSFVNDTKVTETILKSGDRISLGRRNGGIE
                     LMFEAITPKEEQETSQEPVAVKPTPAPPDDPHQIMSVIDHQQTRYLNTSLIEQAKMMN
                     NNTFNRLRALYEITSTILSMQSRNDLCSKLLELLFDLLPAERGVIMLVDGSSDALSIK
                     AAKHRTNTDSSTVHPSMTIVNRVFKDNVATLCLDARSDNRFATQQSIIFQSIRSVICA
                     PISSNTRTWGVCYLDNLTKQKSFEEEELEFLMAVTRQAGIALENLHLLEEQKITFESF
                     VRTLAASIDARDDLTAGHSARVARYSSTIAKHMGLPAEERKIIYYAGLLHDYGKIGTR
                     EAILCKPGKLTPEEYDHMRDHALHTLEILSEIHFSREMENLPLIASSHHENIDGSGYP
                     FGMTGDQIPLGGKIIAVADFFDALTHKRHYREPMPIEEVLELI"
BASE COUNT          551 a          559 c          501 g          468 t
ORIGIN      
        1 cgggtgccgg tcagtttgtt gaacagggtt gattttccga catttgggcg gccaataatg
       61 accacattgg gcagcacgac ggggtctgtg cctatggtga cttgcgtttc gggtacttca
      121 ggtgatggat tcatgatatc tgacataaca acttctttcc gttcaaaagc ccaagtgtag
      181 tcatctggtt gcggtgccgc aaggcttcgg gctgaagaaa ccagggctga agaaaccggg
      241 ctgagggctg aagactcgca agctcggggc aattgaaggg atgagggatg aaggataagg
      301 gatgaaataa aaccaattct tcagcccaat ttcttcagcc cgacttcttc agcccgatgt
      361 cttcagcccg acttcttcag cccgccgttt tcaacccgct gtctcctcaa gatagggtga
      421 ttctgattgc accatgaaaa gcaaaatatg ctatgctctc cgccggcttc tacctcccaa
      481 tcaactacaa tttgttatgt ttttgcaatg gcagcactac cttgcgggat gatcgctcgc
      541 tcggcacaag agaaattgta tcacccattc acattagaaa gagcaggtct gggggctata
      601 tgagtgagaa tcttgaccaa aatgcctggg gcacccttcg ctatcgggac gtcactggaa
      661 aagaacgggt cgtcgtactc aacaaagcac catttaccat tggacgactt ggcgataatc
      721 accttcaact tgatgatccc tccatttcac gtcatcacgt tgaactcgtc tttgatggaa
      781 aaacctatcg catgcgtgac aaacaaagca cctctgggag ctttgtcaat gacaccaaag
      841 tgactgaaac gattctgaaa agcggtgacc gcatcagcct tggtcggcgc aacggcggaa
      901 tcgaattgat gtttgaggcc atcaccccga aagaagagca agaaaccagt caggaaccag
      961 ttgcggtcaa accgaccccg gcccctcccg atgatcccca tcaaatcatg tcggtgattg
     1021 atcaccagca aacccgttac ctcaatactt cgctcatcga acaggcgaag atgatgaaca
     1081 acaacacgtt caatcggttg cgggccctgt atgaaatcac cagcacgatc ctctcgatgc
     1141 agagccgaaa cgatctgtgc agcaaattgc tcgaactcct ctttgatctg ctcccggctg
     1201 aacgcggcgt catcatgctg gtggatgggt catcagatgc cttgagtatc aaggcggcca
     1261 agcaccggac gaacaccgat tcaagcaccg ttcatccgag tatgaccatc gtcaaccggg
     1321 tctttaaaga caacgtggcg acactgtgcc ttgatgcccg gagcgataat cggtttgcga
     1381 cgcaacagag catcattttc caatccattc gctcagtgat ttgcgctccg atcagttcca
     1441 atacccgaac ctggggcgtg tgctatctcg acaacctcac caaacaaaag agctttgagg
     1501 aagaagaact tgaattcctg atggcggtca cacgacaggc tggaatcgca ctggaaaatc
     1561 tgcacctgct cgaagaacaa aaaatcacct ttgaaagctt tgtgcggaca ctggctgcct
     1621 caattgacgc ccgtgacgac ctgacagccg gacactcggc tcgtgtggca cggtactcgt
     1681 caaccatcgc caaacacatg gggctgccgg ctgaggaacg caaaatcatt tattatgccg
     1741 gattgctgca cgactacgga aaaatcggaa cgcgtgaagc catcttgtgt aaacccggta
     1801 aattgacccc cgaagaatat gatcacatgc gcgaccatgc gctccacacg ctggaaattc
     1861 tctcggaaat ccacttctcc agggaaatgg aaaatctgcc gttgattgcc agcagccacc
     1921 acgaaaatat tgacggttcc ggctatccct ttggcatgac aggagatcaa attccactcg
     1981 gtggcaaaat tattgccgtg gccgatttct ttgatgcctt gacccacaaa cgccactatc
     2041 gcgaaccgat gcccatcgaa gaagtcctcg aactcattg
//
LOCUS       DAOQDO010001184         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52079_length_2078_cov_2.984182, whole genome shotgun sequence.
ACCESSION   DAOQDO010001184 DAOQDO010000000
VERSION     DAOQDO010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52079_length_2078_cov_2.984182"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PKZ53_17105"
     CDS             complement(<1..1342)
                     /locus_tag="PKZ53_17105"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ42209.1"
                     /translation="MLELCVGFLLFLALLVVTRGRTTPLFIAWLATSPLLSFWARFPW
                     EKSVLTFDRLMVGCLILSFLCEASARRVPGGFRAGLFEIVWVVFAVFVILDLSLRGGS
                     QLFAWKTGIDGFVLPLVVLVIARQGVDIKQLGNRSVLALSLLTVFLVLVGLVELITHQ
                     DLMVYAGSNLYREGMVRVNGPFLADHSYALISVLIGLWLWYWPRISPVTLSPTVQNIR
                     QAASLLALCASALPFFRAVWATAVGCALLPAFWSGSTVRRRQLILGSTVMAGVLAGGT
                     LVLSQTSFFQERVANPDNLYTRLATYQAAWIMFEDNWLTGVGLTRYTDEYNRTYYNED
                     DYNRKYPTVHGETPRNSPHNTLLAIWAELGLVAGTLYGLAFLLLLLEGYFMSQSSVAS
                     VRQAGQLLVTLWIAYWIPGLLLASGYYADLNFYFFALCGLLLGRKRDLHEPVLEG"
     gene            1662..>2078
                     /locus_tag="PKZ53_17110"
     CDS             1662..>2078
                     /locus_tag="PKZ53_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein"
                     /protein_id="HNJ42210.1"
                     /translation="MTYFDLPPVIERMLAVDDKISDLNFSVGRPPQVEINGKLTPVDI
                     KGLRSLTPYQTEIIAMSLMGGNQESAEKLVHTGSVDLSFSLPSRTRFRVNIFSQRGTL
                     SIVMRVIPTDIPTIESLFLPPQLGDISHLKNGIVLLT"
BASE COUNT          590 a          578 c          492 g          418 t
ORIGIN      
        1 taccttccag taccggttcg tgcaagtcac gttttcgacc aagcagaagg ccgcagaggg
       61 caaaaaaata aaagttcaaa tccgcataat acccgctggc cagcaacaga ccagggatcc
      121 agtaggcaat ccacaacgtc accagcaact gaccagcctg acgcaccgaa gccaccgaag
      181 actggctcat gaaatagcct tcgagcagga gcaagagaaa cgccagacca tagagtgtcc
      241 ccgcaaccag ccccaattca gcccaaattg caagcaaggt attatggggg ctattgcgcg
      301 gcgtttcgcc gtggacggtc ggatactttc ggttatagtc atcctcatta tagtacgttc
      361 ggttgtattc atcggtgtat cgcgtcaacc cgaccccggt cagccagtta tcttcaaaca
      421 taatccaggc tgcctgatag gttgccagcc gggtgtacag attatctgga ttagccaccc
      481 gttcttgaaa aaacgaggtt tgggataata ccagcgtgcc accagccagg accccagcca
      541 tcacagttga gcccaaaatc aattgccgac ggcggacggt actgccagac cagaaagccg
      601 gtaacagggc acatccaacc gccgttgccc aaactgcgcg aaaaaatggc aaggcactgg
      661 cacacaatgc cagcaaactg gcagcctgac gaatattctg tacagttggg gaaagcgtaa
      721 cgggcgagat gcgcggccag taccacagcc ataatccaat caacaccgaa atcagcgcat
      781 acgagtgatc tgccaggaaa ggaccattca cacgcaccat gccttcccga tacagattgc
      841 tgcccgcata caccatcaga tcctgatggg tgatcagttc gaccagacca accagcacca
      901 gaaacaccgt cagcaaactc aacgccagaa ctgacctatt gccaagctgc ttaatatcaa
      961 caccttgtcg ggcaataacc agtacgacca gtgggaggac aaacccgtca atgccagttt
     1021 tccaggcaaa gagctggctg cctccccgca ggctgagatc aaggatgaca aacacggcga
     1081 aaacgaccca cactatttca aaaagcccgg ctcggaaacc gcctgggacg cgccgggccg
     1141 aagcctcaca cagaaaagag agtatcaggc agccaaccat cagtcggtca aaagtcagca
     1201 ccgatttttc ccagggaaag cgtgcccaaa atgacagcag tggcgaagtt gccagccagg
     1261 caataaagag tggggtcgtt cgaccacggg tgaccaccag gagagcaaga aacagcaaaa
     1321 aaccaacaca cagttccaac atcagtgttt tgttccggat tgtgaatatg gtgacaagtt
     1381 gttgcgaaga gaagtcgccg acaatctggc actcccaaat tggacaactg gttgccagtc
     1441 gctgtcaaga aagaccttgt ccagccattt aaatgtaaaa tccagaagca tttgaccagc
     1501 ctgaatcaca acggaattga caccggggca agcaagcccg atgcttgcat cttcggtgga
     1561 ttctggtgta ccgtttggct gccaactctt catcatttcg gctgaatccc catcttcaaa
     1621 tcaaaaatgc acagtgaatc gagccaggag gactttctcg catgacgtac tttgacctgc
     1681 ctccagttat tgaacggatg cttgccgttg acgacaaaat cagcgacctg aatttctcag
     1741 tcggacgtcc accacaagtg gaaatcaatg ggaagctgac cccggttgat atcaagggcc
     1801 tgcgcagcct gacgccctat caaactgaaa tcattgccat gtcgttgatg ggtgggaatc
     1861 aggaatcagc cgaaaaactg gtccataccg gatcggttga cttatcattt agccttcctt
     1921 ctcgcacccg gtttcgcgtc aatatttttt cccaacgggg aacgctttca atcgtgatgc
     1981 gcgtcattcc gactgatatt ccaacgattg aatcattgtt tttaccacct caactgggcg
     2041 acatttctca tttgaagaat ggcattgtcc tgctgact
//
LOCUS       DAOQDO010001185         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52093_length_2078_cov_2.365793, whole genome shotgun sequence.
ACCESSION   DAOQDO010001185 DAOQDO010000000
VERSION     DAOQDO010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52093_length_2078_cov_2.365793"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1459
                     /gene="mnmE"
                     /locus_tag="PKZ53_17115"
     CDS             62..1459
                     /gene="mnmE"
                     /locus_tag="PKZ53_17115"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNJ42211.1"
                     /translation="MSRSQTALQDTIVAIATPPGRGAIGMVRLSGLQALVIAKQLFHS
                     RSVLDEVPFRAVRGDLLDLDSSAPIDDGLAIYFPRPRSFTGEDVIEFHCHGAPIVLQR
                     VVQMAIRSGARTALPGEFTMRAFLNGRINLTQAEGIRDLINAQTTYQAQLAVRQTRGE
                     LSFKLQPAKESLLSMIVHMESSVEFVEENLDTHTRNHIIDQLTNLETNLGALVATYKA
                     GHLVRQGVSLAIIGRPNVGKSSLFNALLDSDRAIVTDLPGTTRDTLTESLAISGIPVR
                     LVDTAGIRATTDVVEKIGVERSYAALNEADVVVLVVDVATGIQPEDENLMQLLGERHA
                     VVVLNKTDLPHKVDEFTSAIERQHLDVPVVSVSAKTRSGLHELREAIWQVCSGASLDS
                     QPDMLITDARHAEKLNETLEALSIARDALIEGYSEEVPLAGMHRALGALGEITGEVTV
                     EQIFDRIFATFCIGK"
     gene            complement(1560..>2078)
                     /locus_tag="PKZ53_17120"
     CDS             complement(1560..>2078)
                     /locus_tag="PKZ53_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42212.1"
                     /translation="EGSVASQSDQYTVKEELVLMNSQEIITLIQSLPVQEQMQIIIAA
                     IENLAKPVTRSNLNQKAETEVRNQLKREIIKLLSFLDEITAPTNQPIQLPVSTDEQER
                     QAMKRQQRHEWKETYRTQYAGKYIALDGNRLLGVGNNYVEARKAAIQAGVKDAYIDYM
                     QSLDEEIFMGGW"
BASE COUNT          479 a          520 c          526 g          553 t
ORIGIN      
        1 ttttcagccc caagcccgaa gttttcagcc ccaagcccga agttttcagc cccaaattcc
       61 tatgtcccgt tcccaaactg ctttacaaga caccatcgtg gcgattgcaa cgccccctgg
      121 acgcggtgcc atcggtatgg ttcgcctgag cggcctgcag gcgctcgtca ttgccaaaca
      181 gctttttcac agtcgaagtg tgctagatga agttcccttc cgggcagtgc gtggtgattt
      241 actcgacctg gattccagcg cgccgattga tgatggactg gcgatttact ttccacgccc
      301 acgttcgttt actggcgaag acgtgattga atttcactgc cacggggcac cgattgtgct
      361 ccaacgcgtg gtgcagatgg cgattcgaag cggtgcccgg acggcgcttc cgggcgaatt
      421 taccatgcgc gcgttcctca atggtcgcat caatttgact caggcagagg gcatccgcga
      481 tttgatcaat gcccagacga catatcaggc ccagcttgca gttcggcaaa cccgaggcga
      541 actttcgttc aagctccagc cggccaagga atcgctgctc tcaatgattg tgcatatgga
      601 atcgtcggtt gagttcgttg aagaaaacct cgacacccac actcgaaacc acatcattga
      661 tcaattgacc aacctggaga ccaatcttgg ggcactggtt gcgacctaca aagctggtca
      721 tctggtccga cagggcgtca gtctggccat tattggtcgt ccgaatgtcg gcaaatcaag
      781 tttgtttaat gccctgctcg attctgaccg ggcgattgtc accgatttac caggaacaac
      841 ccgcgacacg ttgaccgaaa gcctcgcgat ttcaggaatt ccagtcaggc tggtggatac
      901 ggcgggcatt cgtgccacaa cggatgtggt tgaaaaaatc ggtgttgaac gcagttatgc
      961 tgccctcaac gaagccgatg ttgtggtcct ggtggttgat gtggcgactg gaattcagcc
     1021 tgaagatgaa aacctgatgc agttgcttgg tgagcggcac gcagtggttg tcctcaacaa
     1081 aactgatctg ccgcacaaag tagatgaatt tacttcggcc attgaacggc aacatctgga
     1141 cgtgcccgtt gtttccgtct cagccaaaac cagatctgga ttgcatgaac tccgcgaagc
     1201 tatctggcag gtctgtagcg gtgcgtcgct tgatagccag ccggatatgc tcatcaccga
     1261 tgcccggcac gctgaaaaac tcaatgaaac gcttgaagcc ctcagcattg cccgtgacgc
     1321 gttgattgaa ggatattcag aagaagttcc actggcggga atgcaccggg cgctgggagc
     1381 acttggggaa atcactggtg aagtgacggt cgagcagatt ttcgaccgga tttttgcgac
     1441 gttttgtatt gggaagtaat gagagctagg aaagggcaaa gaaaatgcaa gtatttcaat
     1501 cgcttaactt atacaattca gggtcatata gatgggtaaa actcagttga tacgtcacat
     1561 taccatcctc ccataaagat ttcctcatca agcgattgca tataatcaat gtaggcatct
     1621 tttacgccgg cttgaatcgc tgctttgcgg gcttcgacgt agttgttacc gactcccagt
     1681 agccgatttc catcaagcgc aatgtatttt cccgcatatt gagtgcggta ggtttctttc
     1741 cattcgtgtc gttgctgccg tttcatagcc tgcctttcct gctcatctgt actgactggt
     1801 aattgaattg gctggtttgt tggtgcagta atttcgtcca agaaagaaag tagcttaatg
     1861 atttcccgtt tgagttggtt acgaacttca gtttctgcct tttgattcag gtttgacctg
     1921 gttactggct ttgcgagatt ctcgattgct gcaattataa tttgcatttg ctcctgaacc
     1981 ggaagactct gaatcaaggt gatgatttcc tgactattca taagaactaa ctcctccttg
     2041 actgtgtatt gatcactttg gcttgcaact gaaccctc
//
LOCUS       DAOQDO010001186         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52113_length_2077_cov_3.640455, whole genome shotgun sequence.
ACCESSION   DAOQDO010001186 DAOQDO010000000
VERSION     DAOQDO010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52113_length_2077_cov_3.640455"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            456..>2077
                     /locus_tag="PKZ53_17125"
     CDS             456..>2077
                     /locus_tag="PKZ53_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42213.1"
                     /translation="MNRFSNLEHFIVDIEDSTTLLKLYLDRLRRAPSKAATYCEAICQ
                     HSEEIAQAVHLLKRELYTADGSLSQGISFHLAASIESLSDLAELIQYSALRLATQQVT
                     NPTEVTDSVTQATSCFEGVVRSLSNIEVPFDLSQVNRITHHLRTLLTVGSISAQANAT
                     SPLQGPSTLSPPPPQEAEVLFSAEDDPFDELALGEDFFDDVIGGLDNALDSALRGSED
                     AHSFDVHAMTTGELSEAAAAEMLTGSGHAFGHHEEYAIETPSDDLRDLFANIAAAYVQ
                     PVKEFIAELRRGKVSKEWLEICRPSVQSIARAAESMEYYELFRRLRSFDEFLAGAQQE
                     PDNRAIKSNWQTKLMKAYADLEEFLPQTFQIPEDSESPEGIIINSLLKQVKGVGQGTI
                     KKLFSIGMTSLDVYFVASPGDIAAAAGIKIWLAERICERFRQYQQQLQQLSEAGNQES
                     KRLHEISECLEELKRQQFLFKRATLEDWYSQSDSSEKKRFRKDRQQVMWRLNVLLAEL
                     GQMELIQEIKNLVFEKRIERLQEFLDAQQFANR"
BASE COUNT          523 a          543 c          516 g          495 t
ORIGIN      
        1 ctcttgcgca tggaaatcga ggtaccggaa tcactggctg aattgattta aaaatgggga
       61 tttgcatacc ggaaaacgag tacaaatttt tgtctcgctc tctgcatttc cagggaagtt
      121 gtgttagagt gtgctgcttt cacggcagca ggcacctttc cactccaaaa ccacacattt
      181 gatcagcaac catccctgat ggttgacgtt gtggtcagga tactctttac cggaaagcat
      241 cacgtctcac tccattcagg aggaccttta actcatttat ttgagcgacg aaaggacgtc
      301 ctgatcattc acatattgct ctgatcccac acgtcttgat ggcttagcca tgaggtgtcc
      361 tgtcgaaaac tacgtgtccg tcacaatata attccgtaga ggggcggcgc tcctcctcga
      421 ccccaaggcc gaggttcagg cgctgaagaa atttgatgaa ccggttcagc aatcttgaac
      481 actttattgt cgatattgaa gacagcacca ccttgctgaa gctttatctg gatcggctcc
      541 gacgtgctcc cagtaaagcc gcaacctact gtgaagccat ttgccagcat agcgaggaaa
      601 tcgctcaggc ggtgcatctg ctcaagcgcg agttatatac cgctgacggt tcgctttctc
      661 agggcatttc gtttcacctg gctgccagta ttgaaagcct gtccgatctg gcagaactga
      721 tccagtactc ggccttgcgg ttggctaccc aacaggtaac gaaccccacc gaagtgactg
      781 actcagtcac ccaggcaaca tcttgttttg aaggggtggt gcgcagctta agcaatattg
      841 aagtaccgtt tgatctgagc caggtgaacc gaattaccca ccatttgcgc accctgctca
      901 cggttggctc gatctcagcc caggccaatg ccacaagtcc gttacagggc ccctcaaccc
      961 tttcgccacc gccgccccag gaagccgaag ttttatttag cgccgaagat gatccgtttg
     1021 atgaactggc gctgggcgaa gactttttcg atgacgtgat tggtgggctt gataatgcgc
     1081 tggatagcgc cctgcgtgga tccgaagacg cgcatagttt tgatgttcac gccatgacca
     1141 cgggcgaact ctcagaagcc gccgccgccg aaatgttgac cggcagcggt catgcttttg
     1201 gacatcacga agaatatgcg attgaaaccc caagcgatga cctgcgggat ttatttgcca
     1261 atatcgctgc ggcgtacgtc cagccggtca aggaatttat tgccgaacta cgacgcggta
     1321 aggtttcaaa agaatggctc gaaatctgcc gaccttcggt tcaaagcatc gcccgcgccg
     1381 ccgagtcaat ggaatattat gaactgtttc ggcggttacg ctcttttgat gaatttctgg
     1441 ctggtgctca gcaagaacca gacaatcgtg ccatcaagtc caactggcag actaaactca
     1501 tgaaggccta tgccgacctc gaagaatttc tgccgcaaac tttccagatt cccgaagact
     1561 cagagtcccc cgaaggcatt atcatcaatt cgttgttaaa acaggtcaaa ggtgttggtc
     1621 agggaaccat caagaaattg ttctcgattg ggatgaccag cctggatgtg tatttcgtgg
     1681 cgtcacctgg tgatattgca gcggcagcag gcattaaaat ctggctggct gaacgcattt
     1741 gcgagcgatt ccgccagtat cagcagcaac tccagcaact gagcgaggct gggaaccagg
     1801 aaagcaaacg cctgcacgaa atcagcgaat gcctcgaaga actcaaacgt cagcagttcc
     1861 tgttcaagcg cgccacactc gaagactggt acagccagag tgactcatcc gaaaagaagc
     1921 gtttccgcaa agaccgccag caggtgatgt ggcggctcaa tgtcttgctg gccgaacttg
     1981 gtcaaatgga actcattcaa gaaattaaaa atctggtttt cgaaaaacgc attgagcgtc
     2041 tccaggaatt tttagatgcc cagcaatttg ccaatcg
//
LOCUS       DAOQDO010001187         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52118_length_2077_cov_3.175074, whole genome shotgun sequence.
ACCESSION   DAOQDO010001187 DAOQDO010000000
VERSION     DAOQDO010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52118_length_2077_cov_3.175074"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKZ53_17130"
     CDS             complement(<1..322)
                     /locus_tag="PKZ53_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42214.1"
                     /translation="MRVLPIRVKFKRGIPDPKFLERKLTETTGVHVTVTPFHEAAGCP
                     NYFIHFRPGNTGKFDGVVTLSISTDTISVEPFQDDALQKCPNYFFFHTLAVLEDAGGT
                     PHFGI"
     gene            complement(531..1295)
                     /locus_tag="PKZ53_17135"
     CDS             complement(531..1295)
                     /locus_tag="PKZ53_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42215.1"
                     /translation="MQKSLRLLCLCTLLVLATFTFPTLSIRTIQAGSPLPATRQNPFE
                     HEFSFDFSDGRQGWTAGFADSTTDVINREKVVFKLKKLPKNLGERRKVLFIGGTNQTD
                     DLFMFIKRQLTGLRPDTIYSLQFDVQIASNAPTGCVGAGGAPGDGVYFKVGASPIEPV
                     ADPQTTLLNIDKGNQSQGGKDVITIGTIATTGPNCDLSTYEFKTLGTDGAKFQAKTDA
                     SGNIWVLLGTDSAFESRTELFYSKIKILLNEATDGQ"
     gene            complement(1419..1835)
                     /locus_tag="PKZ53_17140"
     CDS             complement(1419..1835)
                     /locus_tag="PKZ53_17140"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HNJ42216.1"
                     /translation="MSETHSQDSADLLGRISSIASFACAIHCILEPFAIGLFPLVGMS
                     FELDEWMEISLIGVAIFFGLITLLPDYYRRRNNLKAILPFVAGVLLLITAQVFFKERT
                     AIELPAALIGAALIITAQTWNRRINHTHTHACTHNH"
BASE COUNT          540 a          468 c          540 g          529 t
ORIGIN      
        1 aaatgccaaa gtgtggggtt ccacctgcat cctcaagaac tgctaaagta tggaaaaaga
       61 aatagtttgg gcacttttga agtgcatcat cctggaaggg ctcaaccgag atggtatcgg
      121 ttgagatcga aagcgtgacc accccatcaa atttccctgt gtttccaggc cggaaatgaa
      181 tgaagtagtt tggacatccg gcagcctcat gaaacggtgt gaccgtaacg tgcacacctg
      241 tggtttcagt cagtttccgt tcaagaaact tcgggtctgg gatgcctcgt ttgaacttaa
      301 cccggatggg caggacccgc atcgtggtac ctcaactttc cttcaacaca caaagagcac
      361 agcgtgaatg tttgattgta cacgctgtgc tctggatttg atcaagtttt ctgcttgagg
      421 aaaggtcagt ttacatcagg ttttgttcaa agatgctcgc ctgagtttcc ccacttaaaa
      481 cctgcataat cagttggtta gatggttgtc aacgcgttga cactttttca ttactggcca
      541 tcggtggctt cattgagcag gattttgatt ttggaataga acagctcggt tcgactttca
      601 aaggctgagt ccgtaccaag caaaacccaa atgttaccac tggcatcagt tttggcctga
      661 aatttggcgc catcagtgcc cagcgttttg aactcgtagg tgctcaaatc acagtttggc
      721 ccggtggtcg caatcgttcc aatcgtaatg acgtctttgc cgccctgaga ctggttgcct
      781 ttatcaatgt tgagcaaggt tgtttgtgga tcggcaaccg gttcaatcgg ggaagcccca
      841 accttgaaat aaacaccatc tcccggcgcc ccgccggctc cgacacagcc ggttggggca
      901 ttagaggcaa tctggacgtc aaactggagt gaataaatcg tatccggtct gagtccggtc
      961 aactgccgct tgatgaacat aaacagatcg tcggtctggt tggtcccgcc gatgaagagc
     1021 acttttcgtc gttcacccag gtttttcgga agcttcttga gtttaaaaac gaccttctcg
     1081 cgattgatca catccgtggt gctgtcggca aacccagccg tccagccctg tcggccatcg
     1141 ctgaagtcaa aggaaaattc gtgttcaaat ggattctgac gggtggctgg aagtgggctt
     1201 ccggcctgaa ttgttcgtat tgaaagtgtc ggaaaggtaa acgttgccag cacgagaaga
     1261 gtgcataaac aaagcaaacg caaagatttc tgcatacttt tgcacgttcc tgattgttat
     1321 tttttgggga tagtattgac tggaaggtgt ggggtgctac gttagaattg gcaaaaaagt
     1381 tcacctttcc aacctgacac ccgtgactga cagggacttc aatggttgtg ggtgcaggcg
     1441 tgcgtgtggg tatggttgat tcgacggttc caggtttggg ccgtaatgat cagggcggcg
     1501 ccgatcaagg ctgcgggtaa ttcaatggcg gtgcgttcct tgaaaaaaac ttgagctgtg
     1561 atcagaagta acacgcctgc gacaaaagga agaatggctt tgaggttatt ccgacggcgg
     1621 tagtagtcag gcagcagggt aatcaatcca aagaaaattg ccacgccaat cagggatatt
     1681 tccatccact cgtcaagctc aaaactcatc ccaaccagtg gaaacaggcc aatcgcaaaa
     1741 ggttcaagta tgcaatgaat cgcacaggca aacgaggcaa tggagctaat ccggcccagc
     1801 aggtcagccg aatcctgaga gtgtgtttct gacacgggcg ttgattttcc tttcgacaag
     1861 aatgtgaaac cgatttctca aattgggaga gaggtgaaac tcgtggcaat ctgaccaaaa
     1921 aaccagatac tgactggttg tggccggttg caaaagactt tttgaatgaa tggttgaaat
     1981 taacccatca aaagacaatt gacggttatg aatggcactg aaagcgagtg tggcatatgc
     2041 cacacctgat gctgcatatg ccacaaattt tgacctg
//
LOCUS       DAOQDO010001188         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52122_length_2077_cov_3.099407, whole genome shotgun sequence.
ACCESSION   DAOQDO010001188 DAOQDO010000000
VERSION     DAOQDO010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52122_length_2077_cov_3.099407"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="PKZ53_17145"
     CDS             complement(<1..1986)
                     /locus_tag="PKZ53_17145"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNJ42217.1"
                     /translation="MIASVKAASAEMQPESLQLAAQWLDKVKSDPYLQRIFDRSAFPK
                     GSNWWIVGGDHTTTGNPMLASDPHLSLSTPSVWYEIHLNVANDPERGPMNVNGVSFPG
                     IPGVVLGANDRICWGATVNILDTGDAFQEELVLDRKTSVPKFSKFNGKKEPVIVLPQT
                     YRVNRIGDGTADTLETATVPPQAAVVVLLPRRNNGPIINLVPDSKKKTKGVGISIQYS
                     GFRATRELDAFLSWNRAGSVEEFKEGLQFFDFGAQNFACADRDGNIGYFTSAEMPLRE
                     DLQKLGRVDGMPPFLLRDGTGVRKNEWLPVTNRQPGQALNYEILPFAEMPQVVNPSAG
                     FITNCNQDPVGTSFDNNPLNQLRKGGGIYYLSPGYADGNRQGRIQRLLQAKLANGGKV
                     SLQDLQAMQHNHQMLDAEVLTPFILTAFQNASAASAPDALKALGQDAGIREAVERFQG
                     WDFSSPTGIREGYDPDDDWRNLPEPSAAEIEHSVAAATYAVWRSQMVQNVIDTTLSRV
                     GAGFALPPGDLAMGPLRNLLDKFPTAKGKGASGVNFFEVTGVSDPDAARDIIILRSLR
                     QGLDLLASPLMAPAYARSTNQNDYRWGRLHRITFDHPLGGPFDVPNAGGFSSLGNGLP
                     GLAKAGGYEVLDASTHSVRMSNTGSGTFGGGPSRRFVG"
BASE COUNT          454 a          592 c          580 g          451 t
ORIGIN      
        1 gccgacgaag cgccgtgacg ggcctccgcc aaaggtgcca gagccggtgt ttgacatccg
       61 aaccgagtgc gtcgaagcgt ccagcacctc gtagccgccc gccttcgcca gcccaggcaa
      121 gccattgccc aggcttgaaa atcctcccgc gttgggcaca tcaaatggac ctcccaacgg
      181 gtggtcaaag gttatccggt gcagtcgtcc ccaacggtag tcattctgat tggtcgagcg
      241 cgcataggcc ggggccatca acggactcgc cagcaagtcc aggccctggc gcagactccg
      301 caagatgatg atgtcgcggg cggcatccgg gtctgaaaca ccggtgactt caaagaaatt
      361 cacacctgaa gcgcccttcc ctttggcggt tggaaattta tcgagcaaat tccggagcgg
      421 ccccatcgcc aggtctcccg gaggcagcgc aaacccggca ccgactcggg ataacgtggt
      481 atcaatcacg ttctgaacca tctggctccg ccacacagca taggtggcag ccgcgacgct
      541 gtgctcgatt tccgcggctg atggttcagg caaattgcgc cagtcgtcat ccgggtcata
      601 gccttcacgg atgcccgtcg gagagctgaa atcccaccct tgaaaccgtt cgacggcttc
      661 gcgaattccg gcatcctgac cgagtgcttt caaggcatcc ggggcactgg cggctgaggc
      721 attttggaag gcagtcaaaa taaaaggcgt caatacttcg gcatccagca tttgatggtt
      781 gtgttgcatc gcctgcaaat cctggagcga gactttgccg ccgtttgcca gtttggcctg
      841 taacaggcgt tgaatccggc cctgacggtt gccatccgca tatccggggc tgagataata
      901 aatcccgcca cctttccgaa gctgattcaa cgggttgttg tcaaacgagg tcccgactgg
      961 atcctgattg cagttggtga taaacccggc tgatggattc accacctggg gcatttcggc
     1021 aaacggcagg atttcataat tcagcgcctg ccctggctgg cgattggtca cgggaagcca
     1081 ctcgtttttg cgaacgccag ttccatcacg gagtaaaaat ggtggcatcc cgtcgacacg
     1141 acccagcttt tgcaaatctt cgcggagcgg catctcggca ctggtgaagt acccgatgtt
     1201 tccatcccta tcggcacagg caaagttctg cgccccgaag tcaaaaaact gcaatccttc
     1261 cttaaattct tcaactgaac cggcacgatt ccaggacaaa aatgcgtcga gttcgcgggt
     1321 cgcccgaaat cccgaatact gaatgctgat gccgacgcct ttggttttct ttttggaatc
     1381 ggggaccagg ttgataatcg gaccgttgtt tcggcgcggg agcaatacca caacggcggc
     1441 ctggggcgga accgtcgcgg tttccagcgt gtcagccgtc ccatcgccaa tccggttgac
     1501 gcgataggtt tgcggcagca cgatgacggg ttcttttttt ccgttgaatt tggaaaattt
     1561 cggcacggag gttttacggt ccaggaccag ttcttcctga aaagcgtctc cggtatcgag
     1621 aatgttaacc gtggcgcccc agcaaatccg gtcattggcg cccagcacca cgccgggaat
     1681 acctgggaat gacaccccgt tgacgttcat cggcccgcgc tcggggtcgt tggcaacatt
     1741 gagatggatt tcataccaga ccgaaggcgt gctcaatgaa agatgcgggt cgctggccag
     1801 catcggattt ccagtcgtcg tgtggtcacc accaacaatc caccagttgc tgcctttcgg
     1861 aaacgccgaa cggtcgaaaa tccgctgtaa atatggatcg gatttgactt tatcaagcca
     1921 ttgtgctgcc agttggaggc tttcgggttg catctcagcc gaagcggctt tcactgaagc
     1981 aatcaaatct tgaagcggtc gggagtcaac ggttgaggac cgaaggccag ctttttcaaa
     2041 aaaatccggg attgagggcg atgcttcaaa cggcgca
//
LOCUS       DAOQDO010001189         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52171_length_2076_cov_2.908956, whole genome shotgun sequence.
ACCESSION   DAOQDO010001189 DAOQDO010000000
VERSION     DAOQDO010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52171_length_2076_cov_2.908956"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..527)
                     /locus_tag="PKZ53_17150"
     CDS             complement(81..527)
                     /locus_tag="PKZ53_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42218.1"
                     /translation="MEGHVKLVGILHVIFGLVGALGSLVLMVAFFFFGAGAVSFLLTQ
                     GKGGDSVAAAGVVGVITLIVVVLVAVMIVWSFLTVLVGWGVMKLKNWARIVAIMLAIF
                     NIISALPSAFSVVGLVWTIICSPLAIYTLWVLMSENGAKVFQRSAY"
     gene            complement(540..1919)
                     /gene="radA"
                     /locus_tag="PKZ53_17155"
     CDS             complement(540..1919)
                     /gene="radA"
                     /locus_tag="PKZ53_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNJ42219.1"
                     /translation="MAKPIKTIFSCQQCGYQSRKWLGKCPDCNAWNSFTEERETKSTP
                     VTAAAAGYGSIGQPVKPSRYTDVPSQDDTRVSSGVAEFDRVLGGGIVPGCLVLIGGDP
                     GIGKSTLLLEVAEKMSRGGHRVLYISGEESERQIKLRGERLGLKPGDLHLLPETCLER
                     IFDVVGNLKPGMIVVDSVQTAYSERLDSAPGSVSQVREVAGQFLLFSKQQTIPVFLIG
                     HITKEGTLAGPKTLEHIVDTVLYFEGERHHNHRIIRAVKNRFGAANELGMFEMTSTGL
                     IPVKNPSELFLQQRPLNVSGSVVTACMEGTRPLLVELQALVSSTKYGTGRRMVQGVEQ
                     NRVSLLIAMLEKRVGLQLIGDDVFVNVAGGIELDEPAVDLGIVAAIVSSFNNKALDNA
                     TVVFGEVGLAGEIRSTGQPAARIKEVAAMGFTRCILPKGNLAGLEAPKGLELIGVRSV
                     IEALDALFG"
BASE COUNT          530 a          600 c          472 g          474 t
ORIGIN      
        1 gtttcttcat tcttcattct tcattcttca ttcttcattc tcaattcttc attctccatt
       61 cttcattctt cattcttcat tcaataggct gatcgttgaa aaactttggc gccgttttca
      121 gacatcaaca cccagagggt gtaaatggcc agtggactgc aaatgatagt ccagacgagg
      181 cccacgacgg aaaaggctga aggcaatgcc gaaatgatat tgaagatagc cagcatgatc
      241 gccacgattc gggcccagtt cttcaatttc atgacacccc acccaaccag cacggtgaga
      301 aaggaccaga cgatcatcac ggctaccagg acaaccacaa tcagggtgat gactccgacc
      361 acgcctgcag ccgccactga atccccaccc tttccctgag tcaacagaaa cgataccgcc
      421 ccagccccaa agaaaaagaa cgccaccatc aacaccaatg atcccaaggc gccaacaagt
      481 ccgaaaatca catggagaat tccaaccagt ttgacatggc cttccataca atcactcctt
      541 tacccaaaga gggcatccag tgcttcgatc acggatcgaa cgccaatcaa ttcaagccct
      601 tttggggctt ctaaccccgc caggttgcct tttggcaaaa tgcaccgcgt aaatcccatg
      661 gcggcgactt ccttaatgcg ggctgcgggc tggccagtag atcggatttc accggccagt
      721 ccgacttcgc caaacaccac ggtggcatta tccagggctt tgttgttaaa actggagacg
      781 attgccgcga caatgcccag atcaaccgct ggctcatcga gttcaatccc acctgccacg
      841 ttgacaaaca catcatcgcc aatcagttgc agcccaaccc gtttttcgag catggcaatc
      901 aagagtgaga cccggttttg ttcgacgccc tgcaccatcc gtcggccagt gccgtatttg
      961 gtcgaactga ccagtgcctg caactcgacc agcagtggcc gggtgccttc catacaggcc
     1021 gtcacgactg agcccgaaac attgagcggg cgttgctgta aaaacaactc cgaagggttt
     1081 ttgaccggaa tcagcccggt actggtcatc tcaaacatgc ccagttcgtt ggccgcgcca
     1141 aaccgatttt tcactgcccg gataatccgg tggttgtgat gacgctcacc ttcaaaatag
     1201 aggactgtat ccacaatgtg ctcaagggtt tttggaccag ccagtgttcc ttccttggtg
     1261 atgtggccaa tcaaaaacac agggatagtt tgttgtttgg aaaataataa aaactggccg
     1321 gcgacttcac gaacctgcga cacgctgccg ggtgccgagt caagccgttc ggagtaggcg
     1381 gtttgaaccg aatcaaccac gatcatgcca ggcttgagat ttccgaccac atcaaaaatg
     1441 cgctccagac aggtttccgg taataaatgc agatcgcccg gcttgagtcc caatcgttcc
     1501 ccgcgcaatt taatctggcg ttcggattct tcaccgctga tatagagcac ccggtggccg
     1561 ccacggctca ttttttcagc gacttccagc agaagggtgg attttccaat ccctggatcg
     1621 ccaccaatca aaaccagaca tcctggcaca atgcctccgc ccaaaacccg atcaaattca
     1681 gccacaccgg aagacactcg ggtgtcatcc tggcttggca catctgtata gcgcgacggt
     1741 tttaccggtt gaccaatgct cccatacccg gcagccgccg ccgtcaccgg cgtagacttg
     1801 gtttcccgtt cttcggtaaa ggaattccag gcattgcagt caggacattt ccctaaccac
     1861 ttgcgtgact ggtagccaca ttgttgacag gaaaaaatgg ttttgattgg tttggccata
     1921 aagtaccagg gctcagggct tggggctcag ggctgggggt tttggagaaa gacaaacagg
     1981 atttaaagga ctcaaagaac tcaaattcga aaaatcggaa acctgacaag gtgacaaggt
     2041 gattttttca tccctcatcc ttcatccctc atccct
//
LOCUS       DAOQDO010001190         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52250_length_2074_cov_3.733036, whole genome shotgun sequence.
ACCESSION   DAOQDO010001190 DAOQDO010000000
VERSION     DAOQDO010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52250_length_2074_cov_3.733036"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PKZ53_17160"
     CDS             <1..563
                     /locus_tag="PKZ53_17160"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ42220.1"
                     /translation="LRDLTSNPNKQIRRDAEMAVTRIEQELTRQGRTPTSSTTIAQSR
                     PVPKPAAPVTAPVKPSKASAVLDEDYRPTRPKEDSLRTSNTDTSSSESPSVTIIRPVR
                     ILSSDKPRYTELAARHKVAGDIVLNVEFKADGTIGDIEVVKGLGFGLDDSAIQTAKRL
                     RFQPAERNGQRVDSISKVQYSFVQIN"
     gene            642..1163
                     /locus_tag="PKZ53_17165"
     CDS             642..1163
                     /locus_tag="PKZ53_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNJ42221.1"
                     /translation="MMNRIEIYRGDITRLAVDAIVNAANSSLLGGGGVDGAIHRAAGP
                     ELVFECRLLGGCPTGQARLTKGYRLPAKFIIHTVGPVWNGGQQGEPELLAQCYRNSLS
                     LAATHTIETLAFPAISCGIYGYPIKQATAIAVAETCQFLTTHSYPTKVIFVCFELPVC
                     QAYQDALQPFTNS"
     gene            1824..>2074
                     /locus_tag="PKZ53_17170"
     CDS             1824..>2074
                     /locus_tag="PKZ53_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ42222.1"
                     /translation="MTNQPSLSRTALAWVRRMSVAFCVPAMLLFCLTTAFAQTDTARL
                     QGTVADQNGGVLSGATVTVTNLGTNREVSVQSSESGDYI"
BASE COUNT          524 a          580 c          472 g          498 t
ORIGIN      
        1 ccctgcggga cctgacctca aatccaaata agcaaatccg acgggatgct gaaatggcag
       61 tcacccgcat tgaacaagaa ctgacccgtc aaggccggac cccaacgagt tcaaccacca
      121 tcgcccaatc cagaccagtc ccgaaaccag ccgcccccgt cacagctcct gtcaaaccaa
      181 gtaaagccag tgcggtactt gacgaagact atcgcccaac ccgaccaaag gaagattcgc
      241 tgcgcaccag caacactgac acctcaagtt ctgagtcacc ttccgttacc atcatccggc
      301 cagttcgcat cctttcaagt gacaaaccac gctataccga gcttgctgct cgccataaag
      361 tggccgggga tattgttttg aatgtcgagt ttaaagccga tggcaccatt ggcgatatcg
      421 aagttgtcaa agggctgggg tttggcctcg atgacagcgc catccaaacg gccaaacgac
      481 tcagattcca gccggctgaa cgcaatggcc agcgcgttga cagtatcagc aaagtccaat
      541 actcgtttgt tcagatcaat tgatcccggt tccgaggttc ctggaaccga tccttctgcc
      601 tcaattcagc tacgctacgt taccctgtca ctctatcacc gatgatgaat cgaattgaga
      661 tctatcgtgg cgacatcacc aggctggcgg tggatgcaat tgtaaatgca gccaacagct
      721 ctctgctggg tggaggtggc gtggatggtg caattcatcg ggccgcaggt ccagaactgg
      781 tttttgagtg ccggttgctc ggtgggtgcc caaccggaca ggccagactc accaaaggct
      841 atcggctccc ggcaaaattt atcattcaca cggttggccc agtctggaat ggcgggcagc
      901 agggtgaacc agaattgtta gcccaatgtt atcgaaacag cctgtcactg gcagcaactc
      961 acacgataga gacactggcc ttcccggcaa tcagttgtgg aatttatggg tatccgatca
     1021 aacaggcgac agccattgca gtggctgaga cctgtcagtt tctcacgact cactcatatc
     1081 caacgaaagt gatttttgtc tgttttgaat taccagtttg ccaggcgtac caggatgccc
     1141 ttcaaccgtt caccaattca tagcctgatc tgtcacctcc ggtgccctct gatttttaca
     1201 attcccaccc gccccagatt ccaagacgcg ctgcgtaatc acataagttg atataaactt
     1261 agaacaatat ttaccagtgc ggccaaaccc tagaaaaatc aatttgatac gggtcattct
     1321 ttcctggcta actcatttca tttcctgcgt caaaaaattc aaatgatccg atgttacagc
     1381 catctcgctg cacaaagttg acttgacggt atccgtaaca tttaggtaac gtaacccaac
     1441 atttgctctt gctttagacg ttcttccatt gccctcaaat tcccttcaac ttcaggcaaa
     1501 ttctaagcac ccggtcgtca ctagccagcg actgttggtg gcaggaacga atcttagcca
     1561 gaatgttttg gtgtaatgag gattcctacg ttggaagaat gtttcacgaa agatcaagtc
     1621 caaatcttgt tgccagccct tccccctcta ccaacacctc ggctggtaac cagagcgctt
     1681 gtgttctgac gcggaagtgc cccttcttac actgcgtcat tcaaccaacc ccccctgttg
     1741 ggactgcccc attgaaattt caagaacgtc aagcatccac tttggtatgt gtaataccaa
     1801 ctcacgtgtg aggagatttg gaaatgacaa atcaacctag tctttcccgg acagcactcg
     1861 cgtgggtccg cagaatgtca gtggcattct gtgtgcctgc gatgcttctg ttttgtttga
     1921 caacggcctt tgcccaaact gacaccgccc gtttgcaagg aacggtcgct gaccagaatg
     1981 gtggcgtgtt gtcaggcgca accgtcacgg tgaccaattt agggacaaac cgtgaagttt
     2041 cagttcaatc cagcgaaagc ggcgactata tcat
//
LOCUS       DAOQDO010001191         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52263_length_2074_cov_2.889549, whole genome shotgun sequence.
ACCESSION   DAOQDO010001191 DAOQDO010000000
VERSION     DAOQDO010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52263_length_2074_cov_2.889549"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1522
                     /locus_tag="PKZ53_17175"
     CDS             356..1522
                     /locus_tag="PKZ53_17175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ42223.1"
                     /translation="MFSFKHSRWLLIFLIWTVVGLFFTSQGYLFHTVASIEQFNLGKW
                     LFREFTYCYLFALATPGIWWLADKISIDRQNYGRGLLVHIPMSLVTSMAIKGLHQMLL
                     VLLVDDPRMVGNNPLLNITSASFSFVTLLKFTYYTLDTGALIYWVILSLNHTSRYIQR
                     YQNEEARSARLEQQLTQAQLEALKMQLQPHFLFNTLHSVSELLHEDVEAADEMIANLG
                     TFLRMTLENSGNHEVTLQQELDFLRCYLEIEQVRFRDRLTVHYEIEPDTLQASVPNLI
                     FQPIVENAVRYAVAPRPAGVISIRAQRENGWLKLEVEDDGPGLPLNERSETSITEGLG
                     LTNTRSRLFQLYGHQARFQLGNGQPQGTRVSLEIPFRPGTAVPAPAELTRAVLS"
     gene            1519..>2074
                     /locus_tag="PKZ53_17180"
     CDS             1519..>2074
                     /locus_tag="PKZ53_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42224.1"
                     /translation="MIKTIIVDDEQLARNRIRRMLKTDPSILVVGEFASGEEALIAIK
                     AQPPDLLFLDVQMPGMDGFGLLSALEENELPVVVFVTAFDQYALKAFEAFALDYLLKP
                     FDRKRFEKTVQRAKQVIEHQRLSRTSQNLAALLENLKPQTSYLERLAVRQGDGLRFIK
                     IESVDWIEAEGNYVKLHTGQESHLL"
BASE COUNT          528 a          533 c          499 g          514 t
ORIGIN      
        1 caaaatataa caaattcaac aacttagctt tctgataaat aaagagtatg cgttcaaaac
       61 ggcatcacca caagttgaag aggtcgtcca gttgtcattc caatcaaaaa ctgtccccac
      121 tggcgccgca tggaattaaa tgcaataatc aatccagcaa ataaattgta aactcattca
      181 atttaaccag ccgattcaac ttaaatctcc cgaatcagag tttaaggatt taaaacccca
      241 actctctggt aaatatgttc tgggagcgcc ggcaggacgc cggcgttccc aggggggaat
      301 ctttttctga aaagcgatat actgcggcat cctttgatct gcaggctctc ataccatgtt
      361 ttccttcaag cattcgcgct ggcttctcat tttcttgatc tggacggtcg tcggactgtt
      421 tttcacgtca caaggttatt tatttcatac agttgcgagt atcgagcaat ttaatctcgg
      481 caagtggctg tttcgggaat ttacctactg ttatttgttt gcattggcca cgccgggcat
      541 ctggtggctg gcagacaaga tttcgattga ccgccagaat tacggtcgtg ggctgctcgt
      601 ccatattccg atgagtctgg tcacatcaat ggccattaag ggcctgcacc agatgctgct
      661 ggtcctgctg gttgatgacc cacggatggt cggcaacaac ccgttgctca acattacgtc
      721 ggcatcattt tcatttgtaa cccttttaaa attcacctat tacaccctcg ataccggggc
      781 actgatctat tgggtgattt tgtcgctgaa ccacacgtcg cgctatatcc agcgctatca
      841 aaacgaggaa gcccggtcgg cccgactcga acaacaactc acacaggctc agttggaagc
      901 gctgaaaatg caattgcagc cgcattttct gtttaacacg ctgcactcgg tttccgagtt
      961 gctccatgaa gacgttgagg cagccgatga aatgattgcc aatctgggga cttttttacg
     1021 aatgacactg gaaaactctg ggaatcacga agtgacgctc cagcaggaac ttgattttct
     1081 caggtgttat ctggaaatcg agcaggtacg gtttcgcgac cgattaacgg ttcattatga
     1141 aattgagccc gataccctcc aggccagcgt gccgaatctg atttttcagc caattgttga
     1201 aaatgcggtt cgctatgcgg tggcaccgcg accggctggg gtgatttcga ttcgggcaca
     1261 gcgtgaaaat ggctggctga aactcgaagt cgaagacgat ggccccgggc tgccactcaa
     1321 cgagcgatct gaaacttcaa tcaccgaggg ccttggactc accaacaccc gcagccggct
     1381 gttccagctt tatggccatc aagcccgctt tcaattagga aacggccagc ctcagggaac
     1441 acgggtttcg cttgaaatcc catttcgacc aggcaccgcc gtaccagccc cagccgaact
     1501 aacccgagcg gtactgtcat gatcaagacc atcatcgttg acgacgaaca actggcacga
     1561 aatcgcatcc ggcgaatgct caaaacagac ccatccattc tggttgtcgg tgaatttgcc
     1621 agtggcgaag aggcgctcat agccatcaaa gcccaaccac ctgatttgtt attccttgat
     1681 gtgcaaatgc ctggaatgga tgggtttggg ctcctgtcag ccctggagga aaatgaattg
     1741 ccggtggtgg tatttgttac ggcttttgac cagtatgccc tcaaagcctt cgaagccttt
     1801 gcgcttgatt acctgctcaa accctttgac cgcaagcggt ttgagaaaac tgtccagcgt
     1861 gccaaacagg tgatcgaaca ccaacggctc agccggacca gtcagaatct ggcggccctt
     1921 ttggaaaatc tgaaacccca aaccagttat ctggaacggc tggcggtcag acagggcgat
     1981 gggctccggt tcatcaaaat cgaaagcgtg gattggattg aagcggaagg aaattatgtc
     2041 aaattgcaca ccggtcagga atcacacctg ttgc
//
LOCUS       DAOQDO010001192         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52302_length_2073_cov_2.869673, whole genome shotgun sequence.
ACCESSION   DAOQDO010001192 DAOQDO010000000
VERSION     DAOQDO010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52302_length_2073_cov_2.869673"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKZ53_17185"
     CDS             complement(<1..799)
                     /locus_tag="PKZ53_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ42225.1"
                     /translation="MDFKVQNGPLSELAADVVVIPVFENESLESDVLKSLDELTNGLI
                     STIVGTDEMHGKSSDTAYVHVGDGLKTRRLLLVGAGIEEKFATRTARNLAGTAARIAR
                     KKGATSLAFVQRGGLDPKTFAKCIAEGALIGLYEPDTYKTEGKTEQTVTQIIVNTSEA
                     ELPAAEQGLALGNIVGEATNFTRMLVNEPGMQLPPRKLAEHAEQVAAQYGLEVDILDE
                     ARMAEFGMGALLGVSRGSDEEAKMIVLTYTPENAAEGTETIALIGKGL"
     gene            complement(841..1194)
                     /locus_tag="PKZ53_17190"
     CDS             complement(841..1194)
                     /locus_tag="PKZ53_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNJ42226.1"
                     /translation="MDAEVLREYCLAFPHATESLQWGNHLVFKVGGKMFAILDLDNLG
                     LSFPCSPDDFYSLTEQANIIPAPYSARYHWVLLQKLSALPTFELQEYLQQAYTRTFSK
                     LPKKVQAQLTTTTEQ"
     gene            complement(1342..1818)
                     /gene="smpB"
                     /locus_tag="PKZ53_17195"
     CDS             complement(1342..1818)
                     /gene="smpB"
                     /locus_tag="PKZ53_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016924667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNJ42227.1"
                     /translation="MSNKSSPTPGFKMIATNREAFYHFHILETFEAGLQLVGTEVKSL
                     REGRINLKDAYAIVKDGEGWLLNAHISPYSHGNRQNHDPLRSRKLLLHKAELRKLFNF
                     IQEKGLTLVPTKLYFKNGRVKVEIGVAKGKKLYDKRETEQKRTVERETRAMMKERR"
BASE COUNT          492 a          522 c          504 g          555 t
ORIGIN      
        1 tcaatccttt tccaatcaac gcaatggttt ccgttccttc agccgcattt tccggcgtgt
       61 aggtcagcac aatcatcttc gcttcttcat ccgagccgcg tgacacaccg agcagcgcgc
      121 ccatgccgaa ttcagccatc cgggcttcat cgagaatatc aacctccaga ccatactggg
      181 ccgcgacctg ttcggcatgt tcggccagtt ttcgaggtgg aagctgcatg ccgggttcat
      241 tgacgagcat gcgggtaaag ttggtcgctt cgccgacaat gttcccgagt gccagcccct
      301 gctcggcagc cggcaattcg gcttcggagg tattcacaat gatctgggtg acggtttgtt
      361 cggttttccc ttcagtttta taggtgtcgg gttcatacaa cccgatcaac gcgccttcag
      421 caatgcattt cgcaaacgtt ttgggatcca gtccgccccg ttgcacaaag gcaagcgatg
      481 tcgccccttt tttacgggca atgcgagctg ccgtgccagc caggttgcga gccgtacggg
      541 tggcaaattt ttcctcgata ccagccccaa ccagcagcaa acggcgtgtt ttgaggccat
      601 cgccaacatg cacataggcc gtatcgcttg attttccgtg catttcgtcc gttccgacaa
      661 tagtggaaat caagccgttg gtgagttcat ccaacgattt gagcacatcg ctttcgagcg
      721 attcattttc aaaaaccgga atgaccacta cgtcagcggc caattccgaa agtggtccat
      781 tctgtacctt aaaatccatg aagttgtctc cgttctacct gagattgctt tgcaaaaaaa
      841 ttattgttcc gtagtcgttg tgagttgggc ctgaactttt ttcggaagct tggaaaaggt
      901 tcgagtatat gcctgctgta aatactcttg taattcaaat gtgggaaggg cagaaagttt
      961 ttggagcagc acccagtgat accgggccga atacggcgcg ggaatgatgt tggcctgttc
     1021 ggtcaggctg taaaaatcat ctggggagca gggaaacgaa agccccagat tgtccaaatc
     1081 cagaattgca aacatctttc ccccaacctt aaagaccaga tggttacccc attgcaaact
     1141 ctcggtcgca tgggggaagg ccaggcaata ttcacgaagt acttcagcgt ccataggatt
     1201 tcctgttggg agattcgttt tattgggctc agggctgaag aaaacgggct gaagaaaatg
     1261 ggctgaaaac attgggttca gggtattcaa cccgttttct tcagccccaa gccccaagcc
     1321 cgagttcttc agccctggta ttcatctccg ctccttcatc atggcacggg tttcacgttc
     1381 aaccgtgcgt ttctgctcgg tttcccgttt gtcatagagc tttttgcctt tcgccacccc
     1441 aatttcaacc ttcacccttc catttttgaa atagagtttg gttggcacca gcgtcagtcc
     1501 cttttcctgg ataaagttaa agagcttgcg cagttcagcc ttatgcaaca agagtttgcg
     1561 cgatctgagc gggtcgtggt tttggcgatt tccgtgacta tacggactga tgtgggcatt
     1621 gagcaaccag ccttcgccgt ccttgacgat ggcataggca tccttgaggt tgatgcgacc
     1681 ttcccggagc gatttgacct cagttccgac cagttgcagg ccggcttcaa aggtttccag
     1741 gatgtggaag tggtaaaatg cctcccggtt ggtggcaatc attttaaaac caggagttgg
     1801 tgagcttttg ttcgacatag ggaaaaaagt tcggaaattc gtcagttgtt gataagtcag
     1861 tagtccatca gtcagttgtt gataagtcag tagtcagtag tccatcagtc agtagataaa
     1921 acccagatcg ttaaatccat agaattcttg aaaagaattg ttcggaactg acttaaatag
     1981 aaccaaatag acttgacagg caatccaaaa tcctaacacc tgactactga ctactgacta
     2041 ctgactactg actactgact actgactact gac
//
LOCUS       DAOQDO010001193         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52307_length_2073_cov_2.646680, whole genome shotgun sequence.
ACCESSION   DAOQDO010001193 DAOQDO010000000
VERSION     DAOQDO010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52307_length_2073_cov_2.646680"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKZ53_17200"
     CDS             <1..523
                     /locus_tag="PKZ53_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ42228.1"
                     /translation="ISDIADRTSILALNASIQAAMAGEAGRGFAVVAGEVERLAERSA
                     EATKRISTLIKTIQSETNEAVAAMETTTREVVVGSEVANEAGKSLVEIETVSNRLAEL
                     MQSISMATKQQARGSESVAKAMGDISDITQQTAAGTKQAAVSIRKLSELADNLRVSVS
                     TFKLPSNGHGSTY"
     gene            1028..1903
                     /locus_tag="PKZ53_17205"
     CDS             1028..1903
                     /locus_tag="PKZ53_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNJ42229.1"
                     /translation="MATKTSNPDSKNEVAVTSGQSPAGNAGQSGPSASTTPVADEVPL
                     IQDNSFLIEEAYNQDAGIVQNILTFTRARNGDWLSTYTQEIPIWTLKHQFSYTIPIQH
                     FADASTGIGDIAINYRYQAFGNSEAKYLFAPRFSVLIPTGNRKKDLGLGAVGYQFNLP
                     LTAVHHEKVTTHWNAGLTYTPNARNASGDKADSFGYNFGQSTIWTPTRRFNVLFETLW
                     NTTQSVASPGRVDNEDSLIINPGVRWSHNFKNGTQIVPGISIPIGIGPSKGDVGVFLY
                     LSIEHNWPFFGRSKD"
BASE COUNT          562 a          554 c          493 g          464 t
ORIGIN      
        1 catcagtgac attgctgacc gaaccagcat tctggcgctc aatgcttcaa ttcaagccgc
       61 aatggctggc gaagctggtc gcgggtttgc cgtggtcgcc ggtgaagtcg aacgtctggc
      121 cgaacgatcc gccgaagcca ccaagcggat cagcaccctg atcaaaacca tccagagcga
      181 aaccaacgaa gcagtggccg cgatggaaac caccactcgg gaagtggttg tcggatcgga
      241 agttgccaat gaagctggaa aatcactggt tgagatcgaa accgtgtcaa accggctggc
      301 cgaattgatg cagtccatct cgatggccac caaacaacag gcacgtggtt cggaatcagt
      361 cgccaaagcc atgggcgata tctctgacat tacccaacaa acagccgccg gtacaaaaca
      421 ggctgctgtt tcaatccgca aactttccga actggccgat aatcttcggg tgtcagtcag
      481 taccttcaag ctgccgtcaa acggccatgg aagcacctat taataccagt ttgtagtcaa
      541 tatagcattt cagaaaaaga tttcggttca gagtaacgcc ttcaggcgtg aggtttgacg
      601 agcaggacaa cagatgaacc cctctcgtct aaaaacgata ctctgaacta ttacaaagaa
      661 ccctgaatcc aacattgggc tggtggatct ctccaccagc tcaacaggac aggtctgctc
      721 attttccaaa tttttgatcc ctgattccgg atgtgaaacc gtctcggacg ccgatggggc
      781 actgccaaaa cagtttttag aaatacagca aaccagttgt cacctcgcca ggccggatca
      841 gactcgccgc actctgaccc agatcttcca attgtgacca aacttcaaaa cagttcaccc
      901 ttacaaaaag cgaggccata taatgacaaa tcattccctg ctctttccgg tattactgat
      961 ttttttctca accgcagtgt gttttggtca agcagtttca tccgcactcc cagtccatga
     1021 aaaaaaattg gcgacaaaaa cttcaaatcc agattcaaag aatgaggttg ccgtcacgtc
     1081 cggccagtca cccgctggca atgccggaca gtcgggacca tcggcctcca ccacgcctgt
     1141 cgcagatgaa gtaccgctca ttcaggacaa cagcttcttg atcgaagaag cctataacca
     1201 ggatgccgga attgtccaaa acatcctgac atttaccagg gcccgcaatg gcgactggct
     1261 ctcaacctat acccaggaaa ttccgatctg gacactgaag caccagttta gctacacaat
     1321 cccaattcaa cactttgccg atgcctcaac cggtatcggg gatattgcca tcaactatcg
     1381 gtatcaggca tttggaaatt cggaagcgaa atatttgttt gcaccacggt tttcggtgct
     1441 tatcccaact gggaaccgca aaaaagatct cggactgggg gcggttggct atcaatttaa
     1501 cctgccgctc acggcagttc atcatgaaaa ggtcaccacc cactggaatg cagggctgac
     1561 ttatacgcca aacgccagaa atgccagcgg ggacaaggct gattcctttg ggtacaactt
     1621 tggacaaagc accatttgga ccccaacccg gagattcaat gtgctgtttg aaaccctgtg
     1681 gaacacaacc caatcagttg ccagccctgg aagagttgat aatgaagaca gtttgattat
     1741 caaccctggt gttcggtggt cccacaattt taaaaacggc acccagattg ttccggggat
     1801 cagcattccg attggaattg gcccaagtaa aggcgatgtg ggcgtgtttc tctacctgag
     1861 cattgagcac aactggccgt tctttggcag gtcaaaggac tgatcgttga ctggatagag
     1921 aaggagtttt catgaccgga accagccagt tgatgtcgcc ctcagaacac gaagtctggc
     1981 gcaagttcat tgcagaccgg tgcggtattg atttcggtga aacacggctt cagtacctgt
     2041 gccgccggct ttcggaacgg atgcgggtac tca
//
LOCUS       DAOQDO010001194         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52403_length_2071_cov_2.296131, whole genome shotgun sequence.
ACCESSION   DAOQDO010001194 DAOQDO010000000
VERSION     DAOQDO010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52403_length_2071_cov_2.296131"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1674)
                     /locus_tag="PKZ53_17210"
     CDS             complement(196..1674)
                     /locus_tag="PKZ53_17210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNJ42230.1"
                     /translation="MVEHNVCKIPPAHAGGTDASLPAIYTFNPTHPGASVMAQGTSPS
                     LELTDIQGNVIRSYSMPLARYFFLAVTDAAKAKTALKQVIPLVTSAEEWDEKPEYTFN
                     LAFNYNGLAALGVSADALALFVPEFQKGMKSQAGILGDTGVSSPSNWEPALAGSNLHI
                     MVALYSKTSDARESQSTQLRNAVTESGGLSLLYEQDCAVLTEGREHFGFEDGMSQPSE
                     AGDGLKNMPGQGTPQADGTWKPLQPGEFVLGYPDEAGEQIIAKGPNDLRINGSYLVVR
                     KLKQNVTQFRTFIQQTADQYFGDTSAAMQEQIAAKMIGRWRSGAPLSLTPDKDDPTLA
                     ADESRNNDFRFGKDPVGYGCPRGSHIRRCNPRDALDGTSTVVRRHRMMRRGIAYGPML
                     PPGAKDDGVDRGLFFVAAVADIQRQFQFVQQQWVNRGDFADLDMAEKDAIIGNNNNDG
                     QFSIPSEDMPHMVFDLPRFVVTRGGDYFFLPGIRALGQIAGS"
     gene            complement(1707..1985)
                     /locus_tag="PKZ53_17215"
     CDS             complement(1707..1985)
                     /locus_tag="PKZ53_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42231.1"
                     /translation="MNDPEGLDFVFQFCEGWPGAQPFEPFRDYVRAHQIETTFFYPAY
                     PMASVKQILKALDWKNKTDNYMRELQKPPSPDVGQVPAPAPTETASGE"
BASE COUNT          485 a          529 c          554 g          503 t
ORIGIN      
        1 aaatggttaa gtttcaatca ggtcgtgagc gaaatagtga aatagtgaaa tggtgaggta
       61 gtaaaggagc gattgggttg cggagagaga aattgttcaa ggttgaacag ttatttatct
      121 tgtttttttg caatacttta aagagagcag tttgcacttc tcacgacctt gcaccttcac
      181 gacttcacaa cttcgctaac ttccagcaat ctggccaaga gccctgattc caggcaggaa
      241 aaaatagtcg ccaccacgag tgaccacaaa ccggggcagg tcaaacacca tgtggggcat
      301 atcttccgat ggaatcgaaa attgtccgtc attattatta ttgccaatga tggcatcttt
      361 ttccgccata tcgagatcgg caaaatcacc ccggttgacc cactgttgct ggacaaactg
      421 aaattgtcgc tggatgtcag ccacggcggc aacaaaaaag agtccacggt ctacgccatc
      481 gtctttcgcc cctgggggaa gcatggggcc atacgcaatc ccccgacgca tcatgcggtg
      541 gcgacgaaca acggttgacg tgccgtcgag ggcatcacgg ggattgcacc gacggatatg
      601 tgacccgcgc gggcatccgt acccaaccgg gtcttttcca aaccgaaaat cattgtttcg
      661 cgattcatcc gccgcaagtg tcgggtcatc cttgtctggg gtcagcgaga gcggagcgcc
      721 acttcgccag cggccaatca ttttggctgc aatctgctcc tgcatcgccg ctgacgtatc
      781 tccaaagtat tgatcggcag tttgttgaat aaaggttcgg aactgggtga cgttttgctt
      841 gagtttgcga accaccagat agctgccgtt gatgcgcaaa tcatttggcc ctttggcaat
      901 aatttgttca ccggcttcgt ccgggtagcc aaggacaaac tcacccggtt gaagcggttt
      961 ccaggtgcca tcagcttgag gtgtgccctg gcccggcatg ttctttaacc catctccggc
     1021 ctccgatggc tgactcatcc cgtcttcaaa gccaaagtgc tcgcgacctt cggtcaaaac
     1081 ggcgcaatcc tgttcataca gcagtgatag cccgcctgac tcagtcacgg catttcgaag
     1141 ctgggtactt tgagactcgc gggcgtcact cgtttttgaa tagagcgcca ccataatgtg
     1201 taaattggac ccggccagtg ctggttccca gtttgatggg ctgctgacac cggtatcacc
     1261 cagaattcca gcctgacttt tcataccttt ttggaactcg ggaacaaaca gcgccagggc
     1321 atcggctgaa acgcccaacg ccgccagccc attgtaattg aaggctaaat taaaagtgta
     1381 ttcaggcttt tcatcccact cttcagccga tgtcaccagt ggaatgacct gtttgagggc
     1441 ggttttggct ttggctgcat cggtgacagc cagaaagaaa tagcgcgcca gcggcattga
     1501 ataactgcga atcacattgc cttgaatgtc ggttaactcc agcgacgggc tggttccctg
     1561 tgccataaca cttgctcctg gatgggttgg gttgaaggtg tagattgcag gtaatgatgc
     1621 gtcagtacca ccggcatgag cgggtgggat tttgcacacg ttgtgctcga ccattacaac
     1681 ccacctgctc acgcaggggg tactgactac tcgccggaag ccgtttcagt gggtgccgga
     1741 gctggcacct ggcccacatc cggtgacggt ggtttttgca gttcgcgcat gtagttatcg
     1801 gttttgtttt tccaatccag ggctttcagg atttgcttga ccgaggccat cgggtaggcc
     1861 gggtagaaaa atgtggtttc aatctgatgg gcacggacat agtcgcggaa gggttcaaaa
     1921 ggttgggcac caggccagcc ttcacagaac tggaacacaa aatccagccc ttcgggatca
     1981 ttcactgaga agtcctgaag gtagtcttcc cagccgccat cgaaattgct ggtaaagaaa
     2041 aactatgcca gggactagtg gtaggacgtg g
//
LOCUS       DAOQDO010001195         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52422_length_2070_cov_4.239702, whole genome shotgun sequence.
ACCESSION   DAOQDO010001195 DAOQDO010000000
VERSION     DAOQDO010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52422_length_2070_cov_4.239702"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            559..>2070
                     /locus_tag="PKZ53_17220"
     CDS             559..>2070
                     /locus_tag="PKZ53_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNJ42232.1"
                     /translation="MKQQHQAKFTIISPHGQRTEYPITATPVSIGRNEQNMIVLFDER
                     ASRFHAVIEQNGSSFVIRDDLSRNGTFVNGLELPRKVNRVLIDGDEIRVGNFRLQFHL
                     TQTSAANLDYDDRPIAEGTVVFKSAADLLKPLTTLSGDVSRLASGGFPSVHSGGKPSQ
                     VEAAVSPFTVIDQSFTTEMEILAKRSRILEFVYELNKMLNRVFKIEEIYEKIAEQIFK
                     ISNAGRIIFAQPNEAEVEINWGKYRDESVLKKYDGIPVSRTIIRRVIRDRVSLLSVDP
                     MADPGLKQHDTFRLGRIRSVMCVPMLGQEEKVLGAIYVDRPDPISFTNEDLNFLTALA
                     ANSAMAVENIKVHEKLLHDAEARLAYQRFLPPFVVEQIMADPDSLELGGVNQIVTVLF
                     ADIRGFTTLSERKSPQEIVSLLNRYFERAAAAIFKYNGTLDKFIGDGIMALFGAPKPS
                     DADAVNAIQAAIELKRQVTEFNQELAAENSDIQLTIGISINTGEVTVGYIGSKL"
BASE COUNT          547 a          469 c          508 g          546 t
ORIGIN      
        1 caaaggtcaa tagttgttta tgtcaaaggt caatagttgt ttatgtcaaa ggtcaatagt
       61 tgtttatgtc aaaggtcaat agttgtttat gtcaaaggtc aatagttgtt tatgtcaaag
      121 gtcaataatt catcacttga acactggtat ggtatcagaa aatcaaatct ggataatatc
      181 taaagcgctc ttttcgctat gccagttggc ctccagatca tggttggaga aagagttgct
      241 caaggtctct ccccttaata taaggatatc ccgcaaaaaa agaggccctg ctcgtggaaa
      301 aaatacgaaa gcttaatcga gtgcttttga aatcggcgca tcctgaagta tcatagtcct
      361 ttcaatacca ataaagcgtc tgtattcaat ccccagtgtc gattggcatg gtcttttaac
      421 cgaaacggat ccgactctct ttggcgaggg aagcggcacc cgggctttga tcgatgtccc
      481 aaaccaatga ctcacctatc tttcataaat tctttggatt aaaggattta gatttctaaa
      541 ccaatcgaac caggcaacat gaagcagcaa catcaagcca aatttaccat catttcaccc
      601 cacggccagc gaactgaata cccgattacg gcgacacctg tctccattgg gcgcaatgaa
      661 cagaacatga tcgtgctgtt tgacgaacgg gcatcacgct ttcatgccgt cattgagcaa
      721 aatggaagtt cttttgtgat tcgggacgat ctcagccgga acgggacatt tgtcaacggg
      781 ttggaactgc cccgcaaagt caaccgggtg ttgattgatg gagatgaaat tcgggttggg
      841 aatttccgac ttcagtttca tctgacccag acctcggctg ccaatctcga ctatgacgac
      901 cggcctattg ctgaggggac ggtggtattt aagagtgctg ccgatttatt aaagcctctg
      961 acgacgcttt caggtgatgt ttcacggctg gccagcggag ggttcccttc ggttcactca
     1021 ggcggaaaac ccagtcaggt tgaggctgcg gtctcaccct ttactgtgat tgaccagtcg
     1081 tttaccactg agatggagat tttggcaaag cgtagccgca tcctggagtt tgtttatgaa
     1141 ctcaacaaga tgctcaatcg ggttttcaaa attgaggaaa tttacgaaaa gattgccgag
     1201 cagattttta agatttcaaa tgccggtcga attatctttg cccagccgaa tgaagcagaa
     1261 gtggaaatca actggggcaa ataccgcgat gaatcggtgt taaagaaata tgacggcatt
     1321 ccggtcagcc ggacgattat ccgccgggtg atccgcgacc gggtctcgct cttgtcggtt
     1381 gacccaatgg ctgacccagg actcaagcaa cacgatacat ttcgcttagg ccggattcgg
     1441 tccgtgatgt gtgtcccgat gcttggccag gaagaaaagg tattaggcgc aatttatgtg
     1501 gatcgcccag atccaatcag ttttaccaac gaagatctca atttcttaac cgccctggct
     1561 gccaattcgg ccatggcggt tgaaaacatc aaagttcacg aaaaactttt gcatgacgcc
     1621 gaagcccgtc tggcttatca gcggtttctg ccgccgtttg tggttgagca gattatggct
     1681 gatcctgaca gtctggagtt ggggggcgtc aatcaaattg tgactgtgct gtttgctgat
     1741 attcgtgggt ttacaacgct ctctgagcgg aaatcgccac aggaaatcgt cagcctgtta
     1801 aatcgatatt ttgaacgggc ggcagcggcc attttcaaat acaacggaac gctcgataaa
     1861 tttattggtg acgggattat ggccctgttt ggcgccccaa agccatctga cgccgacgcc
     1921 gtaaatgcca ttcaggccgc aattgaactc aaacggcagg tcaccgaatt taatcaggaa
     1981 ctggccgccg aaaactctga tattcaactg actatcggga ttagcattaa cactggtgag
     2041 gtcacggttg ggtatattgg gtcaaagctc
//
LOCUS       DAOQDO010001196         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52443_length_2070_cov_2.864020, whole genome shotgun sequence.
ACCESSION   DAOQDO010001196 DAOQDO010000000
VERSION     DAOQDO010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52443_length_2070_cov_2.864020"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKZ53_17225"
     CDS             <1..796
                     /locus_tag="PKZ53_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNJ42233.1"
                     /translation="TADTIAALREVKQRGCPVIAICNVQGSMATREAGGTILTHAGPE
                     IGVASTKAFTSQIIALYLFALFLAQQRQTIDTETSLRHVAQLNELPVKIELLLNQDDT
                     ISDLTKELFRSTDFLYLGRGVQFPIALEGALKLKEISYIHAEGFPAGEMKHGPNALID
                     ERLPVVFLAPVEHGNKVSELRYEKTLSNMEEVRARDGQVIAIVTEGDTKAAEIADHAI
                     FIPQTSDLLSAVLAIVPLQLLAYHIAIRRGCDVDQPRNLAKSVTVE"
     gene            1033..>2070
                     /locus_tag="PKZ53_17230"
     CDS             1033..>2070
                     /locus_tag="PKZ53_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019417482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNJ42234.1"
                     /translation="MDQTDLLKLLQELMALPAETEWFEFKEAKNNFHFDNLGEYFSAL
                     SNEASLKKQPAGWLIFGVTDKRQICGSTYRPNRANLDSLKKEVSQHLNNYLTFEEIYE
                     LSTPGGRVILFQIPPAMRGVPTSWKGHFYGRAAESLGPLSLLEIEQIRQQSEGEDWSA
                     GICQAATLDDLDPLAITFARQEFKKKNNRLADESNTWDDLTFLNKAKVCLNGRITRAA
                     IILLGKPESEHFLSPGTARITWILKDKDGIELDYQHFEPPLILAVEKVFTRIRNLTYR
                     YLPDATLFPVELTQYDPWVMRETLHNCIAHQEYPQGGRIQVIEGPESVLFTNPGDFLP
                     GSVEEVIRLDAP"
BASE COUNT          542 a          494 c          502 g          532 t
ORIGIN      
        1 aacggctgat acgattgcgg ccctgcgcga agtcaaacag cgtggttgcc cggtgattgc
       61 catttgcaac gtgcagggtt cgatggcgac ccgcgaagct ggcggtacga ttttgaccca
      121 tgccgggccg gaaatcggtg tggcttcgac caaagccttt acttcgcaaa tcatcgcgct
      181 gtatttattt gcgctctttc tggcccagca gcggcagacg attgacactg aaacgtcact
      241 tcgtcacgtc gcacaactca acgagcttcc tgtcaagatc gagttgttgc tcaaccagga
      301 cgacacgatt tccgacctga ccaaagaact cttccgttcg acagattttc tgtatctggg
      361 ccgtggcgta cagttcccga ttgcgctcga aggcgccttg aaactcaagg aaatcagcta
      421 tatccatgcg gaaggcttcc cagccggtga aatgaagcac ggtccaaatg ccttgattga
      481 cgaacggctg ccggtggtgt ttttagcccc ggtcgaacac ggcaacaagg tttcggaact
      541 gcgctatgaa aagacgcttt ccaacatgga agaagtccgc gcccgtgacg gtcaggtgat
      601 tgcgattgtg accgaaggcg acaccaaagc cgccgaaatc gccgaccacg cgattttcat
      661 tcctcagaca agcgacctgc tctcggcagt actggccatt gttcccctgc aattgctggc
      721 gtatcacatc gcgattcggc gcggctgtga cgtagaccag ccccgaaacc tcgccaagtc
      781 ggtaacggtc gaatagtttc tgaaatggaa cgatctttga gcggaagcaa tgaaaagatt
      841 gaacttaaag gagttaaatc ggatcttttc atcacttccg tttaaggaaa cactctcgta
      901 ctacaacgag gtttctgctt taacctgcga agcagtgtcg gatggtggcc ggaaacctgg
      961 tttctttgtt caaacctcgt tgtagtcctg gttctgagtt tcaaagacct ttgacgtttt
     1021 tgtggtgctc ttatggatca aacagactta ctcaaactat tacaagagtt gatggctttg
     1081 ccggcggaaa cagagtggtt tgaattcaaa gaagccaaaa ataattttca cttcgacaat
     1141 ttaggggaat acttttcagc tttgagtaac gaagccagcc tgaaaaagca accagcaggc
     1201 tggttgatct ttggtgtaac tgataaacgc caaatttgtg gatcaactta tcgtccaaat
     1261 cgtgcgaacc ttgacagctt aaaaaaagag gtaagccagc atctgaacaa ctacctgact
     1321 tttgaggaaa tatatgaact ctcaacgcca ggtggacgcg tgatcctctt tcaaattcct
     1381 ccagctatgc gaggtgtccc aacttcctgg aaaggtcatt tttatggccg tgccgcagaa
     1441 tctcttggtc ctttaagctt gcttgaaatt gagcagattc gacaacagtc agagggtgaa
     1501 gattggtcag ccgggatttg tcaagcagcg acacttgacg atcttgaccc actggcaatt
     1561 acctttgcac ggcaggaatt caagaaaaaa aataatagat tagccgatga gtcaaatacg
     1621 tgggacgatc ttaccttttt gaataaggcc aaagtatgcc ttaatggaag aattaccaga
     1681 gcggcaatca ttttgttggg aaaacctgag tctgagcatt ttttatctcc tggtacagct
     1741 cgtattacct ggatactcaa agataaagac ggcatagaac ttgattacca gcattttgag
     1801 cccccgctta ttctcgctgt ggaaaaagtt tttactcgaa tcagaaactt gacgtatcgt
     1861 tatttacctg atgccacgct gtttccggtt gaacttaccc agtatgatcc ttgggtgatg
     1921 cgcgaaactt tacataattg cattgctcac caggaatacc cgcaaggcgg acgcattcag
     1981 gtcatcgaag ggccggaatc agtattgttc acaaatcctg gtgattttct acctggaagc
     2041 gtggaagagg tgattcgtct tgacgcccca
//
LOCUS       DAOQDO010001197         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52446_length_2070_cov_2.801985, whole genome shotgun sequence.
ACCESSION   DAOQDO010001197 DAOQDO010000000
VERSION     DAOQDO010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52446_length_2070_cov_2.801985"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKZ53_17235"
     CDS             complement(<1..642)
                     /locus_tag="PKZ53_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42235.1"
                     /translation="MAFDKEKSLKLAGKYVQQGKIAAAIEEYRKIAYADPYDLNILNT
                     LGDLHAKNNQPEEAALQFLRVAESYRDSGQTNTAIAVYKKILKLNPSNIEIMLMLADL
                     YTRQKLLIEARQLYLGLIDLHRQNGQNRQALRVMERIANNDPENAVLRFEIAAGYHLE
                     GLNQEAFEFYLQAGQEFFRKGRDTEAVQSFQRALSITPDSKPARKLLVEALIRQ"
     gene            complement(828..1649)
                     /locus_tag="PKZ53_17240"
     CDS             complement(828..1649)
                     /locus_tag="PKZ53_17240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ42236.1"
                     /translation="MKTLIWFAAPAFALLILLETLYDLWQKTHEYEVKDTVTNITLGF
                     VSMFMDLGWKLFIQVPAYWLLYNLTPLRIPQTWWSWGIVMILVDFAYYWFHRFSHESR
                     FFWNFHVVHHSSEHYNLSVAVRQSWFGGAVSWVFYAPIALMGFDPMMIITAYAINLIY
                     QFWIHTKFIKSLGPLEAILNTPAHHRVHHGVNEPYLDKNYAGMLIIWDRMFGSFTPEI
                     ETPRYGIIKPLRSFNPVWANFHAWVEMVETMRTRVGLGEKLRCIWSAPAMLPKAP"
BASE COUNT          515 a          485 c          529 g          541 t
ORIGIN      
        1 ctggcgaatc aaggcttcga ccagcagctt acgtgcaggt ttggaatctg gcgtgattga
       61 aagcgcccgt tgaaatgatt gaacggcttc ggtgtcgcgc cctttgcgga aaaactcctg
      121 gccagcttgc aggtagaatt caaacgcttc ctgatttaac ccttccaggt gataccctgc
      181 cgcaatttca aaccgcaaga ccgcattttc cggatcattg ttggcaatgc gttccatcac
      241 ccgcagggcc tgccggtttt gtccattttg ccggtgcagg tcaatcaggc caagatacag
      301 ttggcgggct tcaatcagca atttttgcct ggtatagaga tccgccagca tcagcatgat
      361 ttcaatgttt gacggattga gcttgagaat ctttttatag accgcaatgg ccgtattggt
      421 ctgacccgaa tcccgatatg attcggccac ccgcaggaat tgcaacgcag cttcttccgg
      481 ctgattgttc ttggcgtgca ggtcgccaag ggtatttaaa atgttgagat cgtaggggtc
      541 tgcataggca attttccgat attcctcgat ggcggcggca attttgccct gctggacata
      601 cttcccagcc aacttcagcg atttctcttt atcgaatgcc atattgtgtg aggtttcctt
      661 cgcgggaggg ggtcggtatt tgtgtgagca actcggctgc aaaggtatac aagccgttga
      721 agattgtcaa tttgcagttt ttttggatgc tcaaacagct cagggacgca gccgtgaagt
      781 tgatgcgtcc ctgaatgttc ttttggctga tctgtgggtg ttggtcgtca cggtgctttc
      841 ggcagcatgg cgggtgcgga ccagatgcac cgcagttttt cacccagccc gacccgggtc
      901 cgcatggttt caaccatttc gacccaggca tgaaagttag cccaaacggg attgaagctt
      961 ctgagcggct tgatgatacc gtaccgcggc gtttcaattt cgggtgtaaa actgccaaac
     1021 attcgatccc agatgatcaa catgccagca taatttttgt cgagataggg ttcgttgacg
     1081 ccgtgatgaa cccggtgatg ggctggtgtg ttgagaatgg cttccagtgg gccgagggat
     1141 ttaatgaatt tggtatgaat ccaaaactgg taaatcaaat tgatggcata ggctgtaatg
     1201 atcatcatgg ggtcaaatcc catcaaagca atcggcgcat aaaacaccca cgacacggca
     1261 cctccaaacc agctctgccg gaccgccaca ctcaggttat agtgctcgct tgaatgatgg
     1321 accacgtgaa aattccagaa aaaccggctt tcgtggctaa accggtgaaa ccagtaatag
     1381 gcaaagtcaa ccagaatcat cacaatcccc cacgaccacc aggtttgggg gatgcgcaat
     1441 ggcgtcaggt tatagagcag ccagtaagct ggaacctgaa tgaagagttt ccagcccagg
     1501 tccataaaca tactgacaaa tccaagggtg atattggtca cggtgtcttt gacttcgtac
     1561 tcgtgggtct tttgccagag gtcatagagc gtttccaaca gaatcaataa ggcaaatgcc
     1621 ggtgccgcaa accagatcaa ggtcttcata aatcaatcag ggggaaatgg tgtccaggta
     1681 aggggttaaa gcgaaaaaag gaatgtattc gctgtatttt acataatttg ggaagacaac
     1741 atccagagat ttgcccacat ctgagccgcg attcaatcat taatcttagg acttgcagaa
     1801 agcacttggt cctcaaccgt tttaagctag aatcgaactc ttgcaaactg caactcacaa
     1861 ctctttatca ttctttaccc aatccggaaa aattctttct ttgaggagac cgattttatg
     1921 gtgatcagga aagtgccttc attctttggg atgacgcgac gtgtggtttt gtttctgtgt
     1981 ggaattgccc tgatggtgtc tggacagtat gtgctgacct ccaccagtgg gaacagtggt
     2041 caggaacgtg agcttccaac gccgatatca
//
LOCUS       DAOQDO010001198         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52648_length_2066_cov_2.395326, whole genome shotgun sequence.
ACCESSION   DAOQDO010001198 DAOQDO010000000
VERSION     DAOQDO010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52648_length_2066_cov_2.395326"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2066
                     /locus_tag="PKZ53_17245"
     CDS             <1..>2066
                     /locus_tag="PKZ53_17245"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ42237.1"
                     /translation="VLQKIQSTLIASPQAQQIQQLEVIETPTMSRATSDETGTSSRIE
                     LRGVPPGYPFYGTIQLEDGGILSPDRLANNGIVVQPALLTQLNVKAGDEIKIGTLNCR
                     IQGVIQSDPASTLSALSFGPRVFMSLETLKQSGLIVFGTRARYRLLLKVPESQVEPLF
                     TSLKESLKGQLVSVNSYRNSQNRITTQFNQAEDFLSLAGLIVVILGGIGVSSVIRVYI
                     TQRFKSIAILKCLGSTSRQIIGTYLTQVLLLALAGSALGIGVAYLAVWATPRYFLTDF
                     PIQVDFHLTASAIGQGLGIGVLIALLFSLIPLLDIRSVKPNLLLRGESGDRKKRFDLI
                     QITAVTVVISGLLSIASWQAGSIKIGAAFLGGLAVTALVLFGVSLLLVSGLRKIRTIP
                     RFVVRQGITNLYRPGNQTRLILLTTGLGVFFIIAVNLIQTNLLKEFNFSLLQNASDMF
                     LIDIQQDQRVGLVELVRPVIGTDIELVPSLRARLIAFDGKPIELETVENASERNRLGR
                     EYTVTYSNKLGSNEQIVDGKFWDATPSTEPEVSIEEMLYQNTGLKVGSTLTVDILGRR
                     ITAKITSLRKVDWRNSRTGFVILFRPGVLDAAPHTYLAGIKGPTDQRARSQFQTLIIK
                     QFPNITVIDALDIINRTKSILGGFTLAVSFIGGFVFLCGVLILVGSIAMTKYHRVYET
                     AILKTL"
BASE COUNT          496 a          500 c          535 g          535 t
ORIGIN      
        1 aggtgcttca gaaaattcag tccacgctga ttgcttcacc acaagcccaa caaattcaac
       61 agcttgaagt cattgaaacg ccgacgatga gccgggccac cagtgatgaa acagggacaa
      121 gctcacggat tgagttgcgg ggcgtaccac cgggatatcc attttatgga acgattcaac
      181 tggaagacgg agggatattg tcacctgacc ggctggccaa caacggaatc gtggttcaac
      241 cagcactttt gactcaactc aatgtcaaag ctggcgatga aattaaaatc ggaacgctca
      301 attgccgcat tcagggggtg attcaatctg acccggcttc aaccttgagc gcactgagtt
      361 ttgggccacg cgtgtttatg agtctggaaa cactcaaaca aagcggcctg atcgtttttg
      421 gcacccgggc gcgttatcgc ctgctgttga aagttccgga atctcaagtt gagccgcttt
      481 ttaccagctt gaaagaatcg ctcaagggtc aactcgtcag tgtcaactcg taccgaaact
      541 cacaaaatcg aatcacaacc cagtttaatc aggcggaaga cttcctcagt ctggcgggtc
      601 tgattgtggt cattttggga ggaattgggg tttcgagcgt gattcgggtc tatatcacgc
      661 aacgctttaa atcaattgcc attttgaaat gcctggggag caccagtcgt caaattatcg
      721 ggacctacct cacccaggtg ctgttgctgg cgctggcggg gagtgcactc gggattggtg
      781 tggcgtatct ggccgtttgg gcaacgccac ggtattttct cactgatttt ccgatccagg
      841 ttgactttca tttgaccgct tcggcgattg gacaggggct gggaattggg gttttgattg
      901 cccttctgtt ttcattgatt ccattgcttg acatccggtc ggtcaaaccg aatttgctcc
      961 tcaggggtga aagcggagat cggaaaaaac ggtttgacct cattcagata acagccgtga
     1021 ccgtggtgat ctcagggttg ctgagtattg caagctggca ggctggatcc atcaaaattg
     1081 gagccgcctt tctgggcggc ctggcggtca ccgctctggt gttatttggg gtgagtctgc
     1141 tgctggtgag cggactgcgg aaaattcgca cgattccccg gtttgtggtt agacaaggga
     1201 ttaccaatct gtatcggcct ggaaatcaaa cccggctcat cttgctcacc acgggtctgg
     1261 gtgtgttttt catcattgcg gtcaatttaa tccagaccaa tcttctgaag gaattcaatt
     1321 tctcgctcct tcaaaatgcc tctgacatgt ttttgattga tattcaacaa gatcaacgcg
     1381 tcgggctggt tgagctggtt cgcccggtga ttggaacgga tattgaactg gttccatcgc
     1441 tccgtgcccg actgattgcc tttgacggga aaccgattga acttgaaaca gttgaaaatg
     1501 cctcggaacg caatcgcctc ggacgtgaat acaccgtgac ctacagcaac aaactcgggt
     1561 caaacgaaca aattgtggac ggaaaatttt gggatgccac cccaagcacc gaacccgaag
     1621 tttcgattga agaaatgctc tatcaaaaca ccgggctcaa agtcggcagc accctgacgg
     1681 ttgatattct gggacgtcgg atcacggcca aaattaccag tctgagaaaa gtagactggc
     1741 gcaactcacg cactggcttt gtgattttgt ttcgcccagg tgtgctggat gccgcaccgc
     1801 atacgtatct ggctggaatc aaaggcccta cagatcaaag agcgcggagt cagtttcaaa
     1861 ccttaatcat caagcaattt cccaatatca ccgtcattga tgcactcgac atcatcaacc
     1921 gcacgaaatc tattttgggt gggtttaccc tggcagtttc ctttattggc ggctttgttt
     1981 tcttgtgcgg cgtgctgatt ctggtcgggt caatcgccat gaccaaatat catcgggtgt
     2041 atgaaactgc gattttgaaa accctg
//
LOCUS       DAOQDO010001199         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52688_length_2065_cov_2.957214, whole genome shotgun sequence.
ACCESSION   DAOQDO010001199 DAOQDO010000000
VERSION     DAOQDO010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52688_length_2065_cov_2.957214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKZ53_17250"
     CDS             <1..747
                     /locus_tag="PKZ53_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42238.1"
                     /translation="VADPDYGPGNGPVLAGKQLLPLVRLKGTAQEGLQIKTFFPNAQV
                     ETAKAATEHFLKTLHRPAILHIATHGDFLEDTALDAPATDENLTTDEGKRKGLTLEHL
                     FEASPLLRSRLFFAGANTGGSGEDDGTLTALEASSLDLWGTKLVVLSACDTGLGDIKN
                     GEGVYGLRRSLVLAGSEAQMMSLWPVSDAGTRQLMVEYYQRLKAGEGRSEALRQTQLK
                     LLKDPRRQHPFYWASFIQSGEWANLDGVRK"
     gene            complement(945..>2065)
                     /locus_tag="PKZ53_17255"
     CDS             complement(945..>2065)
                     /locus_tag="PKZ53_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ42239.1"
                     /translation="IARVEEFVRRNLAGQKTKQAKSRRNQLERMERLEAPTTDGQASN
                     FNLKPVARTGEDVLTLDELQIGYDGKPLAGPFNLTLKRGERLGVIGPNGTGKTTLLKT
                     LLGNLTPVDGDWKWGTGVNPGYYDQRLESLRLDNTVLQELQTDHLNVSEGELRSFLAQ
                     FLFRNDDVYKEIRTLSGGERGRLALAKLIYSRANVLILDEPTNHLDIASCEALEQALN
                     AYTGTCLIVSHDRYFLDRVATQLLYLERNQDYRLFDGSYTEFWELRQSERQAAAEAAR
                     LQAQEERRARAEAEAAARQEEKKPARKNKQKRRAPEAIEADIATAEAALHEVSDRMGT
                     SEVASNPELLAECQSQYEALNARIEALYAEWETVLVAE"
BASE COUNT          444 a          567 c          566 g          488 t
ORIGIN      
        1 gtggcggatc cggattatgg cccgggcaac ggccccgttt tggctgggaa acagttgctt
       61 ccgctggttc gactcaaagg aactgcccag gaagggcttc aaatcaaaac atttttcccc
      121 aatgcacagg ttgaaacagc caaagcagcg accgaacact ttctgaaaac cctgcatcgt
      181 ccggcaattt tgcatattgc cacccacggg gattttcttg aggatacggc tctggatgcg
      241 ccggccacag atgagaacct gacaacggat gaaggaaaac gcaaaggtct taccctggaa
      301 catctgtttg aggccagtcc gctcttgcgc tcgcggctgt tttttgccgg cgccaatacc
      361 ggaggaagcg gtgaagacga tggaaccttg accgccctgg aagcttccag cctggatttg
      421 tggggcacca aactggtcgt actttcggcg tgcgatactg gattgggaga tattaaaaac
      481 ggcgaaggcg tctatggatt gcgtcgctcg ctggtgctgg ccggaagcga agcccagatg
      541 atgagcctct ggccagtgtc agacgccggg acgcgtcagc tcatggttga atactatcag
      601 cgcctcaaag ctggcgaagg ccgctcagaa gcgctccgcc agacccagct taaattgctc
      661 aaggacccac ggcgacagca tccgttttac tgggcgtcgt ttattcaatc aggcgaatgg
      721 gccaatctgg atggagtgag aaagtgagga aagccgattc agggttcagg gttcagggtt
      781 caggggcata agcgccagga taacaggtaa ctgcacctcg aagaggtgca ttcttctgtt
      841 atgttggcac ttatggggct tggggctgaa gacctggttt tctttcatcc ctcatccttc
      901 atccctcatc ccttcgattg ccccaaaccc ggaccctgaa ccccctactc agccaccagc
      961 acggtttccc attcggcata gagcgcctca atgcgggcgt tgagcgcttc atactgagac
     1021 tgacattcgg ccagtaattc tggatttgaa gcgacttcag acgtgcccat ccggtctgaa
     1081 acttcgtgga gtgcggcttc cgccgtggca atgtcggctt cgatggcttc gggagcacgc
     1141 cgtttttgct tgtttttgcg ggcgggcttt ttctcttcct gacgggcagc ggcttcagct
     1201 tcggcacggg cgcggcgttc ttcctgagct tgaagacggg cggcttcagc cgcagcctga
     1261 cgttcagatt gccggagttc ccaaaactcg gtgtaactgc cgtcaaacaa ccgatagtcc
     1321 tggttgcgtt cgagatagag caactgtgtg gcaactcgat ccagaaaata tcggtcgtgc
     1381 gacacgatca aacacgtccc ggtgtaggca ttgagtgcct gttccagcgc ttcgcacgac
     1441 gcaatgtcca gatggttggt cggttcatct aaaatcagca cgttggcccg gctgtaaatc
     1501 aatttcgcca gcgccagccg accgcgttca ccaccagaaa gcgtgcgaat ttctttgtac
     1561 acatcgtcat tgcgaaatag aaactgcgcc agaaacgaac gtaattcgcc ttccgaaaca
     1621 ttcaggtgat cagtctggag ttcctgcaat accgtgttgt ccagtcggag tgattcaagc
     1681 cgctggtcat aatatccagg gttgacacca gttccccatt tccaatcgcc atcaaccggg
     1741 gtcagatttc caagcagtgt tttcagcagt gtcgtcttcc cagttccgtt tggaccaatc
     1801 acgccaagtc gttcgccgcg tttgagcgtc aggttaaacg gaccggccag gggtttaccg
     1861 tcataaccaa tctgcaattc atccagcgtc agcacgtctt cgccggtgcg agccactggt
     1921 ttgagattaa aattgctcgc ctggccatca gtggtcggtg cttcaagccg ttccatgcgc
     1981 tcaagctggt tgcggcggga tttcgcctgc ttggttttct gacccgccag attacgccgg
     2041 acaaattctt cgacgcgggc aattt
//
LOCUS       DAOQDO010001200         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52689_length_2065_cov_2.831841, whole genome shotgun sequence.
ACCESSION   DAOQDO010001200 DAOQDO010000000
VERSION     DAOQDO010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52689_length_2065_cov_2.831841"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PKZ53_17260"
     CDS             <1..1405
                     /locus_tag="PKZ53_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42240.1"
                     /translation="VNINDLFGTASRDIPRFLQTVFVTPRFEVPRTYAQNFALDNTTA
                     AFGIDENLKTPFFHQWNVSLDRELTNNMVLSVRYVGNKSTNLIRGVDYNQVDVLNNGF
                     AADVLRARSNGFLALAQTGRFDPRFNPNINGSQQLTVFPNLVAGGVLTNTGTIQPLIQ
                     RGEAGQLAAIYHQLGLSGNVNFVANPNVFVADVLANNTESNYHALQVELRHRFSNGLT
                     FQTNYTWSKNLTTSPNINPQVLFEPLLDNNQPGLEYARAQTDIPHIYKANVIYELPFG
                     PGKRFNPNNSILRKLVGGWAVTSIINVQSGNPFTIISGRGTLNRTGRSGNNTVNSSLT
                     NEQLKDLIGVFNTPNGLYLINPSVVGPDGRAASPDGRAPFSGQVFFNPSAGSLGTMAR
                     NVFTNPVFTNWDFSFLKVTPITERITTEFRAEFFNFNNHPVFGLDNQNINSAVFGQVG
                     SQLNSPRVVQLAFKIVF"
     gene            1751..>2065
                     /locus_tag="PKZ53_17265"
     CDS             1751..>2065
                     /locus_tag="PKZ53_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42241.1"
                     /translation="MIWCDWKSWKYVALAWGIAFVYFVCQTTVAISLFLPNEPMPWWR
                     IIKGCFLFCLLWLPTTQFAIAMARRFHLEAKKFLVNLLVHFVTGFVVSVVIHSIGIVA
                     MNW"
BASE COUNT          490 a          536 c          484 g          555 t
ORIGIN      
        1 cgtcaatatt aatgacttgt ttggtactgc cagtcgggac attccccgct ttttgcaaac
       61 agtttttgtc acccccaggt ttgaagttcc ccggacctat gcccagaact ttgcgctcga
      121 caataccacg gctgcctttg ggattgacga gaatttgaag acaccgttct tccaccaatg
      181 gaacgtcagt ctggatcgcg aattgacaaa caacatggtc ttgtcagttc gttatgtcgg
      241 caacaagagc acgaatctga ttcgaggggt tgattacaac caggtggatg tcctcaacaa
      301 tgggtttgct gccgatgtcc tgcgggcacg gagcaatggc ttcctcgctc tggcccagac
      361 aggccggttt gatccgcgct ttaacccaaa cattaacggc agtcagcaac tcaccgtgtt
      421 tccaaatctg gtggcgggtg gagttctgac caataccgga acgattcaac ccttgatcca
      481 gcgtggtgaa gccggtcaac tggcggcgat ttatcaccag ttagggttaa gtggcaacgt
      541 aaattttgtg gccaatccaa atgtttttgt ggctgacgtc ctggccaaca acaccgagtc
      601 caattatcac gcgctgcagg ttgaattgcg gcaccggttt tcaaacgggt tgaccttcca
      661 gaccaattac acctggagca aaaacctgac cacctcgccc aatatcaacc cacaggtgtt
      721 atttgaacca ctgctggaca acaaccagcc gggacttgaa tacgcacggg ctcaaactga
      781 tatcccgcat atctataaag ccaatgtgat ctatgaattg ccttttggcc cagggaagcg
      841 gttcaatcca aacaattcaa tcctgcgcaa actggttgga ggctgggccg tgacctcaat
      901 catcaatgtt cagtcgggga atccgttcac gatcatttca ggtcgaggta cgctcaaccg
      961 aacaggtcgt tcaggcaaca acacagtaaa ttcctcgctc accaacgaac aactcaaaga
     1021 cctgattgga gtatttaaca cccccaatgg tctctatctc attaacccat ccgtcgttgg
     1081 accagatgga cgcgccgcat caccagatgg acgcgcccca ttttccgggc aggtgttctt
     1141 taatccatcg gctggctcgc ttgggacgat ggcacgtaac gtatttacca acccggtgtt
     1201 taccaactgg gattttagtt tcttgaaggt aaccccgatc accgaacgca tcaccaccga
     1261 attccgggcg gaattcttta acttcaacaa tcacccggtc tttgggcttg ataatcagaa
     1321 catcaacagc gcggtgtttg gccaggttgg ctcacagtta aacagcccgc gcgtggtgca
     1381 gttggcgttc aagattgtgt tttagttggt gcctggtgcc tggtgtttgg taaccaatac
     1441 cttcgaagaa cgaagcacaa agcacaaagc accagaaaca aattccattt ttttgtaaca
     1501 agctggattt tcggatccag cttgttttct ttttgtggaa attggtccgg ctttagtttt
     1561 tcccgactgg gagggagaat ttttcatttt cttaggaaca aaatacaggc ttcggagtgt
     1621 atagagagca cacacatttc cttccgtcgc acacacattc gtttcagttt ttcacccagg
     1681 ccaaacttca tatcctcaac catcctcaat gttctgtgta ttgtttgcac aggagggaac
     1741 aggactgttc atgatctggt gtgattggaa atcctggaaa tatgtcgccc tggcctgggg
     1801 cattgccttt gtgtactttg tctgtcagac gaccgtggcc atttcccttt ttctgcccaa
     1861 cgaaccaatg ccctggtggc gaatcatcaa ggggtgtttt ctcttttgcc tgctatggct
     1921 ccccaccacc caatttgcca ttgccatggc ccgccgattt catctggaag cgaagaaatt
     1981 tctggtgaac ctgctggtgc actttgtcac cgggtttgtg gtctcggttg ttattcactc
     2041 cattggaatt gttgccatga actgg
//
LOCUS       DAOQDO010001201         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52720_length_2064_cov_3.063216, whole genome shotgun sequence.
ACCESSION   DAOQDO010001201 DAOQDO010000000
VERSION     DAOQDO010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52720_length_2064_cov_3.063216"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKZ53_17270"
     CDS             <1..392
                     /locus_tag="PKZ53_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HNJ42242.1"
                     /translation="EASVAAYHLAFSPNGNLYVTGPTTGGSREVISEITPDGDVKPFH
                     IGLGRPQGLAFDTDGNLYVAASLDGLRGIFKIPSTGGSPQRVLAGNNLVGLAFDDQGN
                     ALLTTTGEVYRAPLGVKGYMPWLDESD"
     gene            548..1213
                     /locus_tag="PKZ53_17275"
     CDS             548..1213
                     /locus_tag="PKZ53_17275"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ42243.1"
                     /translation="MNLGEAAIAFVVFLFSLTIHEAAHAWTSEKFGDDTGRWLGRVSL
                     NPAVHIDPVGTVLFPLMTLLTAGTIYFGWAKPVPVNPSRWRDHFWGNIAVSGAGPASN
                     LILAFIAGALFKILLITGLWTNLAGAGLQEPIGFLLRFMIMLNIGLAVFNMIPIPPLD
                     GSHILSSLLSVISPTLMDAYEGLREYGFVILLVAIVTGLLNMICAPVFKLVTLGLVFI
                     LGL"
     gene            1231..2016
                     /locus_tag="PKZ53_17280"
     CDS             1231..2016
                     /locus_tag="PKZ53_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNJ42244.1"
                     /translation="METPKPVAPAQPLDLEATLLKENSEYDYKIKLATFEGPLDLLLY
                     LIKRDEVDIYDIPIARIAKEYLTYIQALQELDIDLAGEFLVMAATLIHIKSQMLLPKA
                     PASTIEAGDEPEDPRSELVRRLLEHQKYKTAAHMLWSRAEVEQAVFTRPIPEDELDSE
                     VVATVFDLLETFRRVVERRREKVEIEIARDEVTQAQKLIELRSLLSKQKAINVRSLFE
                     ASRSKREMICILLALLELVKEYSLLIVQEKTFGDILVRRQSAR"
BASE COUNT          495 a          513 c          516 g          540 t
ORIGIN      
        1 tagaggcgag tgtcgctgcc tatcatttgg ccttcagtcc aaacggtaat ttgtatgtca
       61 ccggccccac cacaggggga agccgcgaag tcatttctga aattacacct gacggtgacg
      121 tcaaaccgtt tcacattggc cttgggcgcc ctcagggact ggcctttgac accgatggaa
      181 atctgtatgt tgcagcgagc ctcgatgggc tgcgcggcat cttcaaaatc ccttctaccg
      241 ggggttcgcc tcaacgggta ttggctggaa acaacctggt cggactggcc tttgacgacc
      301 agggaaatgc ccttttgacc acgaccggcg aagtatatcg tgctccgctt ggcgtgaaag
      361 gctatatgcc ctggctggat gaaagcgact aaatgacagg gagaaggtaa gtaggatgac
      421 aggctcacac agcgacaaag cgctttctgt cagccgtcag cctttatccc atacaaaaaa
      481 cccggacccc gtacaccgac cgaacaaggt attctgttca gtcggttttt caagaaagag
      541 gtatgttgtg aatcttggtg aagctgccat tgcttttgtg gtgtttctgt tttcgctcac
      601 cattcacgag gcggcacacg cctggacatc tgaaaagttt ggcgacgaca cagggcgctg
      661 gttgggccgg gtttcactca atcccgccgt ccatattgat cctgtgggaa cggttctttt
      721 cccattgatg actttactaa ccgccgggac aatctatttt gggtgggcca aaccagtacc
      781 agtcaaccca tcacgctgga gagatcactt ttggggaaat attgcggttt caggcgcggg
      841 accagcgagc aatttaattc tggcatttat tgctggtgcg ctgtttaaaa tcctgctgat
      901 aactggattg tggacaaatc tggcaggcgc cggtttacaa gagccaatcg gatttttgct
      961 ccgttttatg attatgctca atattggact ggcagtattt aatatgattc caattccacc
     1021 tttagatgga agtcatattc tctccagcct gttgagtgtg atttcaccaa ccctgatgga
     1081 tgcctatgag ggtctgcgcg aatatggttt cgtcatttta ctggtcgcca ttgtaaccgg
     1141 ccttttgaat atgatttgcg caccggtgtt caagctggtg actctgggat tggtctttat
     1201 ccttggtttg taaaggcagc ttcagtcact atggaaaccc caaaacctgt tgcccccgcc
     1261 caaccacttg atctggaagc caccttgctc aaggaaaatt cggaatatga ttataaaatc
     1321 aaactggcca cctttgaggg cccgcttgat ttgctactct atctaatcaa gcgcgatgag
     1381 gttgacatct atgacattcc gatagcccgc attgccaaag aatacctgac gtatattcag
     1441 gcattgcaag aacttgacat tgatctggcc ggtgagtttc tggtgatggc ggcgacactg
     1501 atccatatca aatcacagat gcttttaccc aaagcaccag cttcaaccat tgaagcgggc
     1561 gatgaacctg aagacccccg ctcagaactg gttcgccgac tgcttgagca tcaaaaatac
     1621 aaaacggcgg ctcatatgct ctggtcacgg gccgaagtcg aacaggcggt ttttacccgg
     1681 cctatccctg aagacgaact tgatagcgaa gtggtcgcca ccgtcttcga tttattggaa
     1741 acatttcgtc gcgtcgtgga acgccgccgc gaaaaggtcg aaatcgaaat tgcccgcgac
     1801 gaagtcactc aggctcaaaa gctgattgaa ttgcggtctc tgctttccaa acagaaggcc
     1861 atcaatgttc gctccctgtt tgaagcttca cgctccaaac gtgaaatgat ttgtatcctg
     1921 ctggcacttc tggagttagt caaagaatac tcactgctga tcgtgcagga aaaaaccttt
     1981 ggcgacatcc ttgtcagacg acaatccgct cgctaagggc tggcatcagg tgattggtac
     2041 ttggtacttg gtgactggta cttg
//
LOCUS       DAOQDO010001202         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52812_length_2062_cov_2.809168, whole genome shotgun sequence.
ACCESSION   DAOQDO010001202 DAOQDO010000000
VERSION     DAOQDO010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52812_length_2062_cov_2.809168"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKZ53_17285"
     CDS             <1..796
                     /locus_tag="PKZ53_17285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ42245.1"
                     /translation="GDQSSDSQFPADSPVPGFVQYLPSPLRLLWNVFQKDYFPLLTNR
                     GYMLACLGYAAVTFSVGALVYWAPKWMTNDKGITAQEANNVLGVCAVFGGAIGTIIGG
                     IVGDRLQKRVRGAYLWICAISAALSAIPTVVAVMSPDKMVYQTSIFIAITILFLGNGP
                     INTALVNLVPPTLRTTALGLVVVIIHVLGDGISLSLVGAISTWIQHLAAQGQAFPAIV
                     TLVAQIFHLNPATQSLSVALVLMPLGMVVGGFLYWLGLHTPEGKKL"
     gene            1102..>2062
                     /locus_tag="PKZ53_17290"
     CDS             1102..>2062
                     /locus_tag="PKZ53_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ42246.1"
                     /translation="MASHHEEEAVEKSYDGRLFWRLLRYLKPYRVRISIAIPLITLTS
                     IFELVALNLTMVAVDLYLKPPSDQNLSFFSRLFKNGVDGLGLHFTPANGVLVIGIWYV
                     ILISIVFSLTYVQSVILNSMGQFIMYDMRKEIFAKFQHLALSYYDRHPIGRMISRLTS
                     DVDALNELFTAGFVTLFSDVFTLIGILVFLLMINWKLAMVSMIIIPLLVGVTAWFRIN
                     ATATYREVRTRMARINAFLQEHITGMPVVQLFNREARAMADFDEINDLHRIANVNSVF
                     YYAVFYPAVGLISSVGVALVVWYGGGQVIQGALTLGSLIFFIQA"
BASE COUNT          415 a          534 c          519 g          594 t
ORIGIN      
        1 gggtgaccag tcatccgact cccagttccc ggctgattcc ccagttcccg gttttgttca
       61 atatctccct tcgccgcttc ggttattgtg gaatgttttc caaaaagact acttcccgct
      121 gctcaccaat cgcggatata tgctggcctg tctgggttat gccgccgtca ccttttcagt
      181 tggggcgctg gtgtactggg cgccgaaatg gatgaccaat gacaaaggca tcaccgccca
      241 ggaagccaat aatgtactcg gcgtatgcgc tgtgtttggt ggtgcgattg gaaccattat
      301 tggtggaatt gtcggtgatc ggctccaaaa acgggtacgt ggcgcctacc tctggatttg
      361 tgccatcagc gccgcgctct cagccattcc aacggtggtg gcggtgatgt caccagataa
      421 gatggtgtac caaacctcaa tcttcattgc aattacgatt cttttccttg gcaatggccc
      481 gatcaacacg gccctggtca acctggtgcc cccgaccctg cgaacaacgg cacttggcct
      541 ggtggttgtg attattcacg ttttgggtga tgggatttcc ctctcactgg tcggcgcgat
      601 ttcaacctgg attcaacacc tggcggctca gggacaggca ttcccggcga ttgtcaccct
      661 ggtggctcag atctttcacc tcaaccctgc aacccaatcg ctttcagtcg cactggtgtt
      721 gatgccgctg ggcatggtgg ttggcgggtt cctctactgg cttggactgc acaccccgga
      781 aggtaaaaag ctttaactgc aggctttcag acaaatattt cctgagtatt tgttttggga
      841 cgtctcagaa accattgaag aatgtggatt tgttttctgt gtgttccgtg tattccgtgg
      901 tttccttatc tgaaatttat ggattggaat acgattcggc taaagcacca ggaccgatgt
      961 ttgaccccat ttcttcagcc catcttcttc agcctcaagc ccaagccctc tacaacgcta
     1021 cttgccagga ctggtggcaa tgcacaaccg agaaagcgca taatcgccct tctattttgt
     1081 tcttgaatcg aggttgtgtg cgtggcaagc catcacgaag aagaagctgt tgaaaagtca
     1141 tatgatggcc gcctgttctg gcggctttta aggtatctca aaccgtatcg ggttcgaatt
     1201 tcaatcgcca ttccgttaat caccctgacg tccatctttg aactggtggc gctcaacctg
     1261 acgatggtgg cggttgatct ctatctcaaa cctccctcag atcagaatct ctcctttttt
     1321 tcccggctgt ttaaaaacgg ggttgatgga ttaggccttc atttcacccc agccaatggg
     1381 gtgctggtga tcggaatctg gtatgtgatc ctgatttcga ttgttttctc cctgacctat
     1441 gtccagtccg tgatcctgaa ctcaatgggt cagttcatca tgtatgacat gcgcaaggag
     1501 atttttgcca aattccagca tctggctctc agctactatg accgccatcc gattggtcgg
     1561 atgatttcgc gcctgacctc ggatgtggat gcgctcaacg aactcttcac cgccgggttt
     1621 gtcacactct ttagcgatgt tttcaccctg attggaattc tggtttttct gttgatgatc
     1681 aactggaaac tggccatggt gagtatgatc atcattccgt tgctggttgg ggtcaccgcc
     1741 tggtttcgta tcaacgccac cgcaacctat cgcgaagttc ggacccgcat ggcccgtatc
     1801 aacgcctttt tgcaagaaca tatcacgggc atgcccgtcg ttcagctttt taatcgcgaa
     1861 gcacgggcaa tggctgactt tgacgaaatc aatgacctgc atcgcatcgc caatgtgaat
     1921 tcggtttttt actatgcggt gttttatcca gcggtgggtt tgattagctc agtcggcgtg
     1981 gcgctggttg tgtggtatgg tggcggacag gtaatccagg gagcgctcac gctgggttcg
     2041 ctgatttttt tcattcaagc ca
//
LOCUS       DAOQDO010001203         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52898_length_2060_cov_3.707731, whole genome shotgun sequence.
ACCESSION   DAOQDO010001203 DAOQDO010000000
VERSION     DAOQDO010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52898_length_2060_cov_3.707731"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>2060)
                     /locus_tag="PKZ53_17295"
     CDS             complement(131..>2060)
                     /locus_tag="PKZ53_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ42247.1"
                     /translation="TGPTYDQLQFRRKEDRNSYQTNFELGLKKGPTLPLGISPAHYIG
                     LMIDRLTLPYAKINNFDELPIPFRCVATDFLSGKTEVMRDKSLAQALRATMSIPGLFP
                     PVEREGKVLVDGALLNNIPTDAMRDELKPDIVIAVDVGTPLGDLKAISSFLGILQQSL
                     TIMTLDSDRRNLSKANIIISPQLEKYSLLDFKPVDDIANLGYKAATESESILSMLSID
                     APTWDEYLIARSERQRSDIPIPTGIDVQGTEVADAELIKPDFEDMIGRPIDFDHLEAN
                     LTKYTGVGRYDSLGYEFSPKPKDNTLIISVLEKTYAPPTMNFGLEIDGSDVNNINFTV
                     GTRLTFFDLVKHGTEWRNDVRLGFQTYLASEFFYPLNEIQPFGYSGFFVAPRFSYQRR
                     RQNVFSGDRNIAEYQLDRTGLAFDIGRLDQKQELRFGLEFGHTDANVRAGDPGLPSVD
                     GRFALARIRWAFDGQDSATVPSKGLRTSVEGRWFFDSPNSPGTSGSFPQAEARLSFFK
                     PAFDKGSVFLVSSGGSSFNYDATPTESFTVGGPFRLSSFDRDEFRGNHYFLNSLGYLR
                     PIGELPPLIGKKVFVGGWYDIGGAWGGPLTDASRLRYRQSVSAGLVVDTIFGPFSLIG
                     SVGESGRGKIFFAFGKFF"
BASE COUNT          529 a          548 c          508 g          475 t
ORIGIN      
        1 acaaaaggga cgaaaaggac gaaaaagacg aaaaggacat aaatttcgaa aaccctgaac
       61 cttgaaccct gaaccctgaa ccttgaaccc tgaaccctga accctgaacc ctgaaccctg
      121 aacccggcct ctaaaagaat ttcccaaacg caaagaagat ttttccacgt ccgctttcgc
      181 cgacgcttcc aatcagtgaa aaaggaccaa aaatagtatc cactaccagt ccggcagaga
      241 cactttgccg gtaccgaagc cgactggcat cggtcagtgg cccgccccag gcgccaccaa
      301 tgtcatacca tccgccgaca aaaacttttt tgccaatgag tggtggtaac tctccaattg
      361 gccgcaaata cccaaggctg ttcaggaaat agtgatttcc ccggaattca tcccgatcaa
      421 acgaactcaa ccgaaatggg ccacccaccg tgaaagattc ggtcggagtg gcgtcataat
      481 tgaatgacga accgccggaa ctgaccagaa acactgagcc tttatcaaat gccggtttga
      541 aaaatgacag tcgggcttca gcctgtggga aactgcccga agtcccagga ctgttggggc
      601 tgtcaaaaaa ccagcgacct tccaccgaag tccgcaaccc ctttgacgga actgtggcgc
      661 tatcctgccc atcaaacgcc caccggatcc gcgccagcgc aaaccgtcca tcaacacttg
      721 gaagtcctgg gtctccggcg cgtacgttgg cgtccgtatg tccaaattcc aggccaaacc
      781 gcaattcctg tttttgatca agccgcccaa tgtcaaaggc caggccggtc cggtccaact
      841 ggtattccgc gatgtttcga tcaccagaaa acacgttttg gcggcgacgc tggtagctaa
      901 atcggggcgc cacaaaaaag ccgctgtagc caaacggttg gatttcattc aaagggtaga
      961 aaaactctga ggccaggtag gtctgaaatc cgagtcgcac atcgttgcgc cattccgtgc
     1021 cgtgtttgac cagatcaaaa aacgtcaatc gggttccaac cgtaaaattg atgttgttga
     1081 catcggaacc gtcaatttcc agcccaaaat tcatcgttgg cggtgcatag gttttttcaa
     1141 gcaccgaaat gatcagcgtg ttgtcttttg gtttgggcga gaattcataa cccaggctgt
     1201 cataccgacc aaccccggtg tatttggtga gattggcttc gaggtggtca aaatcaattg
     1261 gacggccaat catgtcttca aagtcaggtt tgatcagttc ggcatcggcg acctctgttc
     1321 cctgaacgtc aattccggtt ggaattggaa tgtccgagcg ctggcgctcg ctgcgcgcaa
     1381 tgaggtattc atcccaggtg ggcgcatcaa tcgagagcat ggacaggatg ctttcacttt
     1441 cagtcgctgc cttgtacccc agattggcaa tgtcatctac cggtttgaaa tccaggagcg
     1501 agtatttttc cagttgtggc gaaatgatga tgttggcttt cgatagattt cgacggtcac
     1561 tgtcaagcgt catgatcgtc agcgactgtt gcaaaatacc cagaaatgaa gaaatggcct
     1621 tcaggtcacc aagcggtgtt ccgacatcaa ctgcaatcac gatgtcaggt tttaattcat
     1681 cgcgcatggc atcggttggg atgttattga gcagggcccc gtccaccagg acttttcctt
     1741 cgcgttccac cggcgggaac agtcctggaa tggacatggt ggcccgcagg gcctgagcca
     1801 gcgatttgtc acgcatgact tcggtttttc cacttaaaaa atcagtggcc acgcagcgga
     1861 acggaatcgg taattcatcg aaattgttga ttttggcata aggcagcgtc aggcgatcaa
     1921 tcatcaaccc gatatagtgc gccgggctga ttccaagtgg cagcgttggc ccttttttca
     1981 agcccaattc aaagttggtt tgataggaat tccggtcttc tttgcgccga aactgcaact
     2041 ggtcataggt tggccctgtc
//
LOCUS       DAOQDO010001204         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52951_length_2059_cov_3.000499, whole genome shotgun sequence.
ACCESSION   DAOQDO010001204 DAOQDO010000000
VERSION     DAOQDO010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52951_length_2059_cov_3.000499"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKZ53_17300"
     CDS             complement(<1..222)
                     /locus_tag="PKZ53_17300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099127.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HNJ42248.1"
                     /translation="MRYIPNASSEREAMLESMGVSNIAELFRGIPETLLLNRPLDIPP
                     AQSEVELIATFKQLAGENRDPESYASFLGA"
     gene            complement(234..626)
                     /gene="gcvH"
                     /locus_tag="PKZ53_17305"
     CDS             complement(234..626)
                     /gene="gcvH"
                     /locus_tag="PKZ53_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNJ42249.1"
                     /translation="MSHYPDDCSYTKDHEWIRVSGDTGRIGITYYAQESLGDVVYVDL
                     PKVGETYKSGAPFGSVESVKAVSELFIPVSGEVLEVNAELADRPELVNNSPYDDGWMV
                     VVRLTSPSEVDALLSATEYEDFINNGKA"
     gene            complement(771..1874)
                     /gene="gcvT"
                     /locus_tag="PKZ53_17310"
     CDS             complement(771..1874)
                     /gene="gcvT"
                     /locus_tag="PKZ53_17310"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNJ42250.1"
                     /translation="MSNQLRLTALGETHRALGAKMVDFTGWEMPVQYTGLIPEHNAVR
                     TAAGLFDVSHMGQLWVNGPAALPLLQRVTCNDVSKLADNQIHYSGLMTEQGTFVDDIL
                     VHRLSAERFFLCVNAANIEKDFNWIAQHASGLDVEVLNHSEAYSQLALQGPKAAEILQ
                     SLIEFDLSSLKYYWCQPEIELFGCHVFLTRTGYTGEDGFELYFAPEHSVGIWEGLLKA
                     GKAYGLVPVGLGARNTLRLEAKMALYGHEIDDTTTPLEADLGWICKLNKGEFLGSDLI
                     QRQKAEGLQRKLAGFEVDDRVPARDGYTVLHDGEEVGRVTSGSPSPYLKKNIGLAYLP
                     LHLTQVGTPIQIFVRGREVAAHVIPTPFYKRQK"
BASE COUNT          529 a          539 c          494 g          497 t
ORIGIN      
        1 ggcgcccaga aatgaagcat aggattccgg atctcgattc tcgcccgcca attgtttaaa
       61 agtggcaatc aactcaactt cgctttgagc tggtgggatg tcgagcggtc ggttgagcag
      121 gagggtctcg gggatacccc gaaataattc ggcgatattg gaaaccccca tgctctccaa
      181 catggcttca cgctcagaac tggcattcgg aatgtatcgc atgtgtagac cagttatgcc
      241 tttccgttgt tgataaaatc ttcgtattcc gtggcagaca gcagggcgtc tacttcgctc
      301 ggagaagtca atcgaacgac caccatccac ccgtcatcat acggtgaatt gttgaccagt
      361 tcaggcctat cagccagttc agcgttgact tccaagactt cacccgagac tggaataaac
      421 aattcactga ctgccttaac ggattcaacc gatccaaacg gagcccctga tttgtaggtc
      481 tcgccgactt ttggtaaatc cacatagacc acatctccca gtgactcttg agcatagtag
      541 gtaatgccaa tgcgacctgt atccccgctc acacgaatcc actcgtggtc tttggtatag
      601 gaacaatcat ccggataatg tgacatactt gtctcttaac tccttgaaag tgaaggttta
      661 gggtttaggg tttgaaaatc gggtgcaacg catttcaaac cggcagaagt gagtgggaac
      721 ggccaccaat cagggtgagt gagaggaggg ctcctttccc cctgatcagg ctacttttgt
      781 cgtttataga acggggttgg aatgacatgg gccgcaactt cacgaccacg aacaaaaatc
      841 tgaatcggcg ttccaacctg agtaagatga agtggcagat aggccagccc gatatttttc
      901 ttcagatagg gcgaggggct tccggaggta acgcgcccga cctcctcgcc atcgtgcaac
      961 acggtgtaac catcacgagc cggaacccga tcatcaactt caaagccagc cagttttcgc
     1021 tgtagacctt ctgccttttg acgttggatc aggtcgcttc ccaggaattc acctttattc
     1081 aatttgcaaa tccatcccag atcggcctca agcggtgttg tggtgtcgtc aatttcgtga
     1141 ccatagagtg ccattttggc ttccaggcgc aacgtattgc gcgcgcctaa cccaactggg
     1201 accaatccat aggcttttcc agccttgagc aagccttccc aaatgccaac actgtgttct
     1261 ggcgcaaaat agagttcaaa cccatcttca ccggtatagc ccgtgcgggt taaaaacacg
     1321 tggcatccaa aaagctcaat ttcaggctga caccagtaat atttgaggct tgaaaggtca
     1381 aattcaatca aagattgcag aatttcagcc gcttttggcc cttgtaacgc caactggctg
     1441 tacgcttcgc tgtgattgag tacttcgaca tccaggccag aggcgtgctg ggcaatccag
     1501 ttaaaatctt tttcaatatt ggcggcgttg acacagagga aaaatcgttc ggcgctcaac
     1561 cgatggacga gaatgtcatc cacaaacgtt ccctgctcgg tcatcagccc ggaataatgg
     1621 atctggttat cggccaattt gctgacatca ttgcacgtca cacgttgcaa taacggcaag
     1681 gcagccggtc cgttgaccca gagctgcccc atgtgactca cgtcaaagag accagccgca
     1741 gtgcggaccg cattgtgttc cggaatcagg cctgtgtatt gaacaggcat ttcccatccg
     1801 gtaaagtcaa ccatcttggc acccagggcg cggtgcgttt ctccaagcgc agtaagtcgt
     1861 aattggtttg acaagagtat gtacctgatt tgagttatga taaggtggcg ggtgaaatca
     1921 agccagaaac taccattgcc ccgccaatgc gtcaagaatg aagcctaaac cactcccgaa
     1981 gaacggaaaa aactccatct ttccgaccac gccgaatggg actgaacttg cgtcctatga
     2041 tcagaaactc aactttatt
//
LOCUS       DAOQDO010001205         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_52979_length_2059_cov_2.297904, whole genome shotgun sequence.
ACCESSION   DAOQDO010001205 DAOQDO010000000
VERSION     DAOQDO010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52979_length_2059_cov_2.297904"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1806..2059
                     /locus_tag="PKZ53_17315"
                     /pseudo
     CDS             1806..2059
                     /locus_tag="PKZ53_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
BASE COUNT          523 a          503 c          566 g          467 t
ORIGIN      
        1 gctcacgcag gtggtactga caagtccttt atttccaacg cagctttgaa cttattgaac
       61 gactgacgac tgaccactgg ctactgacga ctgactggta ttcacttgaa accaatggaa
      121 tctgagagga actcactatg cccacccgtt gctggacgac tatctttcga accccacttt
      181 tcaatctggt gatcatcata cttgggttgt atgccttttc gacaactgtt tccgcacagg
      241 ttgccgatgg ggaatatgcc gtggtcacag ttggcggtaa aaatacctac gatattcaga
      301 ttgaaggcaa atcgtacaat tgttatcgcc acggacgcct gaccggcggc atggtctatt
      361 acattgatcc tcaaaaagga tgggcgggag gatcaccggg ttccacgcaa actgccaaat
      421 acgaattgcc agccgttggg cagaatgtgt atgtggtgct tgattttgat ttcggagacg
      481 gcgccggcaa caaagtgttt aaagccaaag tgacggggat tggttcgcaa gcctttgata
      541 aaggaaaggt gttcctcgac atcgaaattg atctgggcat gttcaagttt gaaggcatgg
      601 cccatcatta cgccaccgaa gccgaataca aagaattggg ttggccggcg ccgcgttcgg
      661 gcaaaaaacc ggatattgat ggcaatgtcg gcagtggaag cgaagaaaaa gtcgcggttc
      721 ccgtaccggc tgaaacggct gattcagcga aaaaacggcc acccgccgag cccggcagcg
      781 ggttcaggat tacatttgac tacggaaaag ccgacaaatc atttaccccg gcggtccggg
      841 cgcaactgga agaagccgct gccttcctca ccagttttat tgctgatgac cgcgaagtaa
      901 ttgtcacggt ggtggcagac ccaaacgtga aagcctttgc ttcggcaggt ccctcggaat
      961 gggaagaaaa caccgaagcc cgctcggtgt cactcgccgg aggaattgcc tttaacccca
     1021 agagtatcaa tgacgtaaat gaaaaagagt cgagcctggt ggccctgacg attcacgaac
     1081 tgctgcacgt gcttgggttt accgattcag ccagagcata tgccaaacac aacaaaaatg
     1141 gacaatttac tggtccgacg acggttaaag tcaataaagg gaatccggtt gaggctcagg
     1201 gcgggcattt ttcacgcagc gtggttgatg ccaatgggat gacgccccgg atggccgatg
     1261 gtggcggcga cctgttgtca attctggatc tggccgtact ggctgacctt gggtatgtga
     1321 ttccatcgct caaaggtgcc actggacccg tggcgcttgg gtttaagctt ggcaaatcac
     1381 agtcaatgac gatgcattat ccagatggtt cgccagcctt tttgattcag gggtacggcg
     1441 gcaatgacac cttgattgcg ggcgatgtga cgctcaaaaa cgggaaaaaa gccacgtttc
     1501 tttttgctgg cggaggcggc gacgacattc tggttggtgg tccgggcgat gatgaaatgc
     1561 gcggtgacaa cagtcaggtc gcttcatatg gcaaagatgg gaaagacata tttatgattc
     1621 atccagatgg cggaaacgat tcgattctgg atcttgaaga tggtcgagat gtgattttgc
     1681 tctcgccgga gtttgggatg tctgagtctg agctggccga acacctcaaa gatgaaaaga
     1741 acctgggtga gaagcctgtt ccaggaactg cctttttcta tcagggcgtg tacctgctca
     1801 aactggacaa gctccagctc agcatcacca acaccgggat gatcgacgcc gggctcaacg
     1861 taaacatgga gatcgaccgg tcgatcgatc gcgataacag catcctggat tactgccggc
     1921 tgcacgacat cacgatccag ccctggtcgc ccttccaata tggcttcttc gaaggcacat
     1981 tcttgggcaa cccgaagttc ccggagctca accagaagat tgacgaaatt gcagcgcagt
     2041 acaacgtcac gaataccgc
//
LOCUS       DAOQDO010001206         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53058_length_2057_cov_2.757243, whole genome shotgun sequence.
ACCESSION   DAOQDO010001206 DAOQDO010000000
VERSION     DAOQDO010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53058_length_2057_cov_2.757243"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="PKZ53_17320"
     CDS             <1..1788
                     /locus_tag="PKZ53_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNJ42251.1"
                     /translation="PAETILDKLNPTYHESARNLLHYLALRRHDLRPLQLRLAALGLS
                     SLGRAESHVLATVDAVLRALHRLIQRDGDLPDPVTPALDFAGGQRLLTDHTERLLGPV
                     PPGREVRIMVTMPSEAADDYMLVHNLVKQGMDCMRINCAHDDTRAWKQMIDHLRHAEA
                     SLGRSCQVVMDLAGPKLRTGPLEPGPTVMKIRPQRDVYGKVVAPARVWLTNEESPVRS
                     PSPADAILPVSADWLAQLEVGDAVKFRDLRGAPRTLHVVDATDDGCWAELKRVAYIVP
                     GIVLHLEPGDHRPETGKTEVGVFAPRENPISLQQGDLLILTRNLKPGRPATVDSRGRI
                     LCPASIGCTLPEVFDDIRAGERIWFDDGKIGGVIERIEADRILVRITQTAARGAKLRS
                     DKGINLPDSALRLSALTEKDIADLRFVVHHADAVALSFANQAQDVDLLREEINRIGGP
                     QPAVILKIETRRGFENLAAMLLTAMRMPCSGVMIARGDLAVECGFERLAEVQEEILWI
                     CEAAHVPVIWATQVLETLARQGMPSRAEITDAAMGHRAECVMLNKGPFVVNAVQVLDD
                     ILQRMQTHQAKKSAMLRELRLAHLFPQIK"
     gene            complement(1808..2056)
                     /locus_tag="PKZ53_17325"
     CDS             complement(1808..2056)
                     /locus_tag="PKZ53_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNJ42252.1"
                     /translation="MFNFEPPVTDEEIRAAALQFVRKVSGFNKPSKANEPAFLAAVEE
                     IATISKNLLNALETTSPPKSREEEAARAQARSAKRFPT"
BASE COUNT          433 a          554 c          606 g          464 t
ORIGIN      
        1 ccggctgaaa cgattctgga caaactcaac ccaacctacc atgaaagcgc ccgcaacctg
       61 ctccattatc tggcgttgcg gcgacatgac cttcgcccgc tccagttacg gctggcggcg
      121 ttggggttat cttcgctggg ccgtgccgaa tcgcacgtgc tggcgacggt ggatgctgtg
      181 ttgcgtgcgc tgcatcggct gattcagcgc gatggggatt taccagatcc agtgacccca
      241 gcccttgatt ttgctggagg tcagcgcctc ctcactgacc ataccgaacg gctgctggga
      301 ccagttccgc caggtcgaga agttcggatt atggtcacca tgcccagcga agccgccgac
      361 gattacatgc tggtccacaa tctggtcaag cagggaatgg attgcatgcg catcaattgt
      421 gcccacgacg acacccgagc ctggaaacaa atgattgacc accttcgcca tgccgaagcg
      481 tcgctgggtc ggtcgtgtca ggtcgtaatg gatctggcgg gaccgaagtt acgcactggg
      541 ccactggaac ctggaccgac ggtgatgaag attcgtccgc aacgcgatgt ctatggaaaa
      601 gttgtggccc cggctcgggt gtggctcacc aatgaagaat cgccagttcg gtctccgtcg
      661 ccggcggatg ccattctccc ggtatcggct gactggctgg cgcaacttga agtcggagac
      721 gcggttaagt ttcgtgacct tcgcggtgcc ccaaggactc tgcacgtggt ggacgccacc
      781 gatgatggat gctgggccga actcaagcgc gttgcctata tcgttcctgg aattgtgctc
      841 cacctggagc cgggtgatca ccgtccggaa actgggaaaa cagaagttgg ggtgtttgcc
      901 ccacgagaga atccaatcag cttgcagcaa ggggatttgc tgattttgac tcggaattta
      961 aaaccgggcc gacccgccac ggtggatagc cgtgggcgaa tcctctgtcc ggcttcgatt
     1021 ggctgcacct tacccgaagt ttttgacgat attcgcgccg gagagcgaat ctggtttgat
     1081 gatggcaaaa tcggcggcgt aatcgaacgc atcgaagcgg accggatcct ggttcgcatc
     1141 acccagaccg ccgcccgagg ggccaaactc cgaagtgata aaggcatcaa cctgcctgac
     1201 agtgcgctac ggttgtcggc actgaccgaa aaggatattg ccgatctccg gtttgtggtt
     1261 catcacgccg atgccgtggc gctctcgttt gccaatcaag cccaggatgt tgatttactc
     1321 cgcgaggaaa tcaaccgaat tggtgggccg caaccggcgg tgattctcaa gattgaaacc
     1381 cgccggggat ttgaaaacct ggctgccatg ttgctgactg ccatgcgcat gccctgttcc
     1441 ggcgtgatga ttgcccgtgg tgatctggcg gttgaatgtg gctttgaacg actggccgaa
     1501 gtgcaggagg aaattctctg gatttgtgaa gccgcgcatg tgccagtgat ttgggcaacc
     1561 caggttttgg aaacgctcgc ccgccaggga atgccatcgc gggctgaaat taccgacgcc
     1621 gcgatgggcc accgggccga atgcgtgatg ctcaataaag ggccctttgt ggtcaatgcc
     1681 gttcaggtac tggatgacat tttgcagcgg atgcagaccc accaggcaaa gaaaagcgcc
     1741 atgcttcggg aactccggct ggcgcatctg tttccgcaaa tcaaatgaag tgagttcaaa
     1801 gagcggttta tgtcggaaat cttttggctg atcgtgcctg tgctcgggct gcctcttcct
     1861 cacgactttt cggaggagaa gttgtctcaa gggcattcag caaattttta gaaatggtgg
     1921 caatttcctc aactgctgcc agaaacgcgg gttcattggc ctttgatggt ttattgaagc
     1981 cactcacttt tctcacaaat tggagcgcgg cggcccgaat ttcttcatcc gtgacaggtg
     2041 gctcaaaatt gaacatg
//
LOCUS       DAOQDO010001207         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53095_length_2056_cov_2.842579, whole genome shotgun sequence.
ACCESSION   DAOQDO010001207 DAOQDO010000000
VERSION     DAOQDO010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53095_length_2056_cov_2.842579"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKZ53_17330"
     CDS             <1..776
                     /locus_tag="PKZ53_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ42253.1"
                     /translation="HPTKGHARLFGHDIVKENRKIRALVGYMPERDAFIAGMSCVGFV
                     AMMAELSGLPPRVALERAHESLFFVGLGEARYRSIDTFSLGMKQMAKLAQAIVHGPQF
                     IILDEPTNGLDYPSRKRMLELVRTIRDSGQACILLSSHLLRDVEESCEEIVILKNGKI
                     VVQCNLEAERKANLKFIEIELGQPNGPFAEGLSQLGCEWALIGERRVKLVLPPGVEIR
                     DLYQIAADRQLHIRRLNYKRDSLEDIFMKAMEATPNGSL"
     gene            763..1623
                     /locus_tag="PKZ53_17335"
     CDS             763..1623
                     /locus_tag="PKZ53_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42254.1"
                     /translation="MAVYDQTYLTYTGPLTSAWKRFLILPRYAYRAVFQSKLFIILFI
                     ISLVFPLLASIWIYMHHNAEAMAILNLGLRDLVPINWRFFYVITQFQSSFATLLTLML
                     APTLVSKDVENNGLALYLSRPFSRLEYVLGKMSVVLILLSAVTWVPVLLLFLFQGYLA
                     GIGWMIENLRFGMAIVVGSWVWILTLCLGSLALSAWIKRKITIQGIMIGFYFATAVFA
                     QVINQLFETKWGYLFSLSEAMGSLWYGLFGQFERMDLPWWGAGLTIGLFFVLSLGLLS
                     RKIRAYEIIN"
     gene            1780..>2056
                     /locus_tag="PKZ53_17340"
     CDS             1780..>2056
                     /locus_tag="PKZ53_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ42255.1"
                     /translation="MNANLIVFDDISKFYGEVLGVNRVNLSIGPGITSFVGPNGSGKT
                     TLMNLMTGLLKPTRGKISVLGVAPDHPEKLFRILGYCSQFDSFPAGMT"
BASE COUNT          456 a          481 c          557 g          562 t
ORIGIN      
        1 atcacccaac caaaggccat gcccggcttt ttggccacga tattgtcaaa gaaaatcgca
       61 agattcgcgc tctggtcggc tatatgccgg aacgggatgc gttcattgcc ggaatgtcct
      121 gtgtcgggtt tgtcgccatg atggccgaac tctcaggtct tccaccgaga gtggcgctcg
      181 aacgggccca cgaaagtctg ttttttgtcg gcctgggaga agcccgctat cggtccattg
      241 ataccttttc actcgggatg aaacaaatgg ccaaactggc ccaggcgatt gttcacgggc
      301 cacaatttat catcctcgac gagcccacca acgggcttga ttacccgtca cgcaaacgca
      361 tgctcgaact ggtgcgcacg attcgcgaca gtggccaggc gtgcattttg ttgtcttcgc
      421 acctgttacg cgacgtcgag gaatcctgtg aagaaattgt cattctcaaa aatggcaaga
      481 ttgtggtgca gtgcaatctg gaagccgaac gcaaagccaa tttaaaattc atcgaaatcg
      541 aattggggca acccaacgga ccgtttgccg aggggctgag ccagcttggc tgtgaatggg
      601 ctttgatcgg agagcgacgg gtgaaactcg tgttaccgcc aggcgtggaa attcgggatc
      661 tttaccagat tgccgccgac cggcagcttc atatccgccg actcaattac aaacgcgatt
      721 cacttgaaga catctttatg aaggcaatgg aggcgacccc caatggcagt ttatgatcag
      781 acgtacctca cctataccgg gccattgacg tcagcctgga agcggttttt gattttaccc
      841 cgctatgcct atcgagcggt gtttcaatca aagctgttta ttattctctt tatcatttcg
      901 ctggtgtttc ctctgctggc aagcatctgg atttacatgc accacaacgc cgaagccatg
      961 gccatcttga acctgggatt acgcgatttg gtcccgatca actggcggtt tttttatgtc
     1021 attacacagt ttcaaagcag ctttgccacg ctgctgacct tgatgctggc accgacgctg
     1081 gtttcaaaag atgtcgaaaa taacgggctg gcgctctatc tcagccgtcc attttcgcgc
     1141 ctggaatatg tcctcgggaa aatgtcggtt gtgttaatcc tgctgtctgc ggtgacctgg
     1201 gtgccggtgt tgctgttatt tttatttcaa ggctatctgg cggggattgg ctggatgatt
     1261 gaaaatctga ggttcggaat ggcgattgtg gttggttcct gggtctggat tttgacgctc
     1321 tgccttggct cgctggcact ttcggcctgg atcaaacgga aaattactat tcagggcatc
     1381 atgatcgggt tttattttgc cacggctgtc tttgcccagg tgatcaatca gctctttgaa
     1441 accaaatggg gatacctgtt tagcttgagc gaggcgatgg gttcgctctg gtatggtctc
     1501 tttggacaat ttgagcgcat ggacctgccc tggtgggggg ctgggttgac tattggcctg
     1561 ttttttgtcc tctcgctcgg tttactgtcc cgcaaaatcc gggcgtacga aattatcaac
     1621 tgaatgaaga atgaagaatg aagaattgag aatgaagaag gcatctggtg cttggtgctt
     1681 ggtgtttggt gctttggacc caattctttc caagaaccaa ggaccaagga ccaaggacca
     1741 agcaccaggt acaaggttca ttctcaaggg gttttacgaa tgaatgccaa tctgattgtt
     1801 tttgacgata tctcgaaatt ttatggcgaa gtgctggggg ttaatcgggt gaatttgtcg
     1861 attggccctg ggattaccag ttttgtcggc ccaaatggat cgggaaagac cacgttgatg
     1921 aacctgatga cgggtctgct caagccgacg cgaggaaaaa tctcggtgct gggcgtggca
     1981 ccggatcatc ctgaaaaact gtttcgaatt ttgggttatt gttcgcagtt tgatagcttt
     2041 cctgctggca tgaccg
//
LOCUS       DAOQDO010001208         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53115_length_2056_cov_2.188406, whole genome shotgun sequence.
ACCESSION   DAOQDO010001208 DAOQDO010000000
VERSION     DAOQDO010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53115_length_2056_cov_2.188406"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKZ53_17345"
     CDS             complement(<1..357)
                     /locus_tag="PKZ53_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ42256.1"
                     /translation="MKLQLKHKLVVGTSIGIVCLVLLVASRYSNQKSPTMAMPIPEVE
                     VVTVEQKDIPIYNEWIGTLDGMVNAEIRAQVSGYLLIQHYAEGSFVKKGQCLFEIDPR
                     PFQVALDQAKARLAEAE"
     gene            complement(425..691)
                     /locus_tag="PKZ53_17350"
     CDS             complement(425..691)
                     /locus_tag="PKZ53_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42257.1"
                     /translation="MTLRIQRSTEADWVVFTLSGRISAEQIETLHSLFALEVAAGFIL
                     DLIEVKLVDREAVKILARWEATGIRLRNCPPFIREWIARERACF"
     gene            complement(1003..1758)
                     /locus_tag="PKZ53_17355"
     CDS             complement(1003..1758)
                     /locus_tag="PKZ53_17355"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNJ42258.1"
                     /translation="MSTNSKKLAGKVAVVTGASKGIGAAIAIHLADEGAAVVVNYASS
                     KEGAERVVSEIVSRGGKAIAVQANVSKKSDIEHLFAETNETFGTLDILINNAGIYEMW
                     PLEAITEDHFHRQFDLNVLGLLLTTQEAVKHFSPTGGSIVNISSIVSTWSPANAAVYS
                     ATKAAVDAITRSLSKELAPRNIRVNSINPALVETEGVQTAGVLEHLKQIVSITPLGRI
                     GQPQDIAPAAVFLASSDSSWMTGETIYITGGLR"
BASE COUNT          523 a          518 c          473 g          542 t
ORIGIN      
        1 ttcggcttcc gccaaccggg ctttggcctg atcaagcgcg acctgaaacg gtcgcgggtc
       61 aatttcaaac aaacactggc cttttttgac aaatgaccct tcggcatagt gctggatgag
      121 caggtaccca ctcacctgag ccctgatttc cgcattgacc attccgtcga gggtgccaat
      181 ccattcgtta taaatcggaa tgtctttttg ctcaactgtc acgacttcaa cttcaggaat
      241 gggcattgcc attgtggggg acttctggtt tgaatatcgg gaagccacca ggagtaccag
      301 acagacaatc ccgatagagg tgccaacgac aagtttatgt ttcaattgta acttcattac
      361 cgtctccatg cttgttttcg gcgtgcctgc ccattgtacg agcccgaaat tcgttgatga
      421 ccgatcagaa gcaggctcgt tcgcgggcaa tccattcgcg aataaagggc ggacaattgc
      481 gcaatcgaat tcctgttgct tcccatctgg ccaggatctt caccgcctca cggtccacga
      541 gtttgacctc aatcaaatca aggataaatc cggcggcgac ttcgagggca aaaagggagt
      601 gtaatgtttc aatctgttcg gcactgattc gcccgctcaa ggtgaaaacg acccaatcgg
      661 cctcggttga tctttggatt ctcagtgtca tggtcgttct cacctataga atttcagata
      721 aagaaaccac ggaatacacg gaatacacag aaaataaatc caaatttttc aatgatttct
      781 aagactccgc acaataaaaa ccctggaaat atttatctga aaagttaagt accttgccat
      841 aagcttcctg agatattgcg tttggtaagc ggttgatttt tccgtgtttt ccgtgttttc
      901 cgtggtgaaa atgtctggaa attttcggta aagtacttat atcttcgttt cctcgtttcc
      961 ctgtctcctg gtcattcagc ccgcaggtcc agagccacca cctcaccgaa gaccgccggt
     1021 gatgtagatg gtttcacccg tcatccagga tgaatctgat gaagccagga aaacagccgc
     1081 cggggcaata tcctgtggct gcccgatacg accgagcgga gtaattgaga caatttgttt
     1141 caggtgttca agcacacctg ccgtttgtac cccttccgtt tcaaccagcg cgggattgat
     1201 cgaattgacc cggatgttcc gtggcgccag ttccttgctc aacgaccggg taatggcatc
     1261 aaccgccgct ttggtggcgc tatatactgc cgcattagcc ggagaccagg tgcttacaat
     1321 agagctaata ttgacgatgc ttccacctgt cggggaaaag tgtttgacgg cttcctgagt
     1381 tgtcagcaac agcccaagga cattcaaatc aaactggcga tgaaaatggt cttcggtaat
     1441 ggcttcgagc ggccacatct catagattcc agcattgttg atcagaatat cgagtgttcc
     1501 aaaggtttca ttcgtttcag caaataaatg ttcgatatcg ctcttttttg agacattggc
     1561 ctggacggca atcgcttttc cgcctcgcga aacaatctcg ctcacgacac gttccgcgcc
     1621 ttctttactg gaagcataat tgaccacaac cgccgcgcct tcgtcagcca ggtggatggc
     1681 gatggccgca ccgattcctt ttgaagcacc ggtgacaacc gcgacttttc cggccagttt
     1741 tttgctattc gtactcataa tattctcctg atacaaaatt gggttcaatt ggcatttcgt
     1801 gaatttcaaa ctacgccata cctgtggcaa ttggggtgcc aatcgtaact cattgaaaac
     1861 aggagaattc acaaactcaa aagttacgaa ccttcgataa ttcgcgaagt ttcgtgaaaa
     1921 tagttcgtga tttttgttgc gtcagaatag gtaggaaaaa ccccgtaaca aagaaactgg
     1981 gaaatttgat tatcaggtgt tgatttcgaa aacccggaac ccggaacccg gaacccggaa
     2041 cccggaaccc ggaacc
//
LOCUS       DAOQDO010001209         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53243_length_2053_cov_2.869870, whole genome shotgun sequence.
ACCESSION   DAOQDO010001209 DAOQDO010000000
VERSION     DAOQDO010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53243_length_2053_cov_2.869870"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2053)
                     /locus_tag="PKZ53_17360"
     CDS             complement(<1..>2053)
                     /locus_tag="PKZ53_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42259.1"
                     /translation="PGLQSPVIAPGGIVVDGAGNLLIADTKSSRIRKLAYDTGIITTI
                     AGTGQSTPNFLIDVNNKLATSVPVSFPSDVTVDNKGDIYLIARFSAQIYKIDAQTGML
                     KVVAGNGVAGFSGDGGPATQARFSNSMYALDIDRSGNIFVVDAGNQRIRRISPQGIVT
                     TIAGTGQPGNTGDNGPATAATINVDVEEIALTAAQCAVDRDGNFFFADSFNSVVRRID
                     ARTGVITRVVGTGTPGTTRDNVVALQNPLQLPIGVFIADTGDLYVTDADSAQVWRVDA
                     TTKIMRLVAGTGTQGVGGEGVIATNSALRFPTAAVIDGAGNIFISDSGNNRVRKIDTR
                     TQIIRTIIGGDTRAIIGDGGSAIRARLVTPTDIAVSTTGNYFVGDPNDGRVRLTQGGT
                     SSIQTIAGNGELNPNQPQTGVPANSVSVNAQGIDVAINGDLLIADQFLGAIWRVNSAG
                     ILSRVAGNLNGSPQDGIPALSAFIQPQDVAVDGAGNIYFSEGETIFGVPSRIRRIDVQ
                     SGILTTVAGTGIPGFSGDGGPAFTAQLNEPEDLAIDRDGNLFICDVQNNRIRRVDART
                     RVITTVAGTGNPNFNGESGAATAINLNSPFGLAVDGGGNLFISDASNLRIRRVQLSTG
                     QLTTIAGIDSLDYKGDGGPARNASLFVTFGLAVDRSGNLLIADTGNNRVRIIKSAASP
                     AV"
BASE COUNT          482 a          558 c          513 g          500 t
ORIGIN      
        1 gaccgccgga gacgctgcac ttttaatgat tcggacccga ttgttgccgg tatccgcaat
       61 cagtaaattt ccacttcggt caacggccag tccaaaagtg acaaacaggg aagcattgcg
      121 ggctggcccc ccatcccctt tataatcaag tgaatcaatc ccggcgatgg tcgttaattg
      181 cccggtagaa agctgaacgc gccggattct caggttggaa gcatcgctga taaacagatt
      241 tccacctcca tccacggcaa gtccaaaagg ggaattcagg ttaattgccg ttgcggcgcc
      301 gctctcgccg ttaaaatttg gattgcccgt accggccact gttgtaatga cacgggttcg
      361 ggcgtcaaca cgtcggatgc gattgttttg gacatcacaa ataaacaaat tcccgtcacg
      421 gtcaatcgcc agatcttcag gctcgtttaa ttgagccgta aaggctggcc cgccatctcc
      481 tgaaaatcct gggattcctg tccccgccac ggttgtgagg ataccggatt ggacatcaat
      541 ccgccggata cgggagggaa ctccaaagat cgtctcccct tcggaaaaat agatatttcc
      601 agctccatcc accgcaacat cctgtggctg aatgaacgct gagagcgctg gaattccatc
      661 ttgagggctt ccattcaggt tgccagccac ccgcgacaat attccagcgg aattgactcg
      721 ccagatcgca cctaaaaact gatcagcgat gagcaggtca ccgttaatgg cgacgtcaat
      781 tccctgggca ttaactgaga cggagttagc cggaaccccg gtctggggct ggtttggatt
      841 gagttctcca tttcctgcga ttgtttgaat cgagctggtt ccgccctgag tcaggcgaac
      901 tcgcccgtca tttggatcgc ccacaaagta attgccagtg gtgctgactg caatatcagt
      961 tggggtaacc aggcgggctc gaatagcact gccaccatct ccaataatcg ctctggtgtc
     1021 accaccaatg atggttcgaa tgatttgggt tcgagtgtca atttttcgga cccgattgtt
     1081 tccagaatca ctgatgaaaa tattccccgc cccatcaatg acagcggcag ttggaaatct
     1141 cagcgccgaa ttggtggcaa taactccttc tccaccaacg ccctgagttc cagtcccagc
     1201 caccaatcgc ataattttcg tcgtcgcatc aactcgccag acctgggctg aatcagcgtc
     1261 ggtgacatat aaatcgccgg tgtcagctat aaaaacgccg attggaagtt ggagggggtt
     1321 ttggagtgcc acgacattat cgcgagttgt gcctggtgtg ccggttccaa ccactcgggt
     1381 aatgacgcct gttctggcat caattcggcg aaccacggaa ttgaaactgt cagcaaaaaa
     1441 gaaatttcca tctcgatcaa ccgcacattg ggccgccgtc agcgcgatct cctcaacgtc
     1501 cacattgatg gttgctgcgg tggctggccc attatcgcca gtatttcctg gttgtcccgt
     1561 tccagcaatc gtggtcacga tcccttgcgg ggatatccgt cgaatgcgtt gattcccggc
     1621 atccactaca aaaatattcc ccgaccgatc aatatcgagc gcatacatgc tgttactgaa
     1681 tctggcctgg gttgctggtc ccccatcgcc gctaaatcca gcaactccat ttccggcgac
     1741 gactttgagc attccagtct gggcgtcaat tttgtaaatc tgagccgaaa atcgtgcgat
     1801 gagatagata tcccctttat tgtcaacggt tacgtcagat ggaaaagaga ctggcactga
     1861 ggttgccagc ttgttattga catcaatcag aaagttgggc gtggattggc ctgttccggc
     1921 aatggtcgtg atgatccctg tgtcatacgc cagttttcga atccgggagc ttttggtatc
     1981 ggcgatcagc aaatttccag cgccatccac cacaattcct ccaggggcaa tgactggtga
     2041 ttgcaggcca ggg
//
LOCUS       DAOQDO010001210         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53247_length_2053_cov_2.798799, whole genome shotgun sequence.
ACCESSION   DAOQDO010001210 DAOQDO010000000
VERSION     DAOQDO010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53247_length_2053_cov_2.798799"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PKZ53_17365"
     CDS             complement(<1..791)
                     /locus_tag="PKZ53_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ42260.1"
                     /translation="MTTESQHPRKLTTLQLVGLIFFTVSGGAYGLESLVQEMNPGWAM
                     VLVLITPLLWGLPTALMVAELASAMPVEGGYYHWVRTALGDFWGVQEGWWTICYTAVD
                     MAIYPVLFVNYLAFFLPVLALDEHGDCTWSVFWLRWGIALVVVLCALGVNLLGARAVG
                     NNALMSILLVLIPFGLLTVLSLSRSGATGTAWAAIKSGWSQPVDLKTIGIGFSVVLWN
                     YCGWDNVSTYAGEVDHPQRAYPRALTIALPLIVVAYVLPLLAGLAV"
     gene            complement(956..>2053)
                     /locus_tag="PKZ53_17370"
     CDS             complement(956..>2053)
                     /locus_tag="PKZ53_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42261.1"
                     /translation="GFDGKFRIGQQFFFNFQVLGTPSRQYFFDADQGSTIYRTGNGIG
                     YSIGLSKKGRYLTVDYRAQGRSKEYRTNVGFTRRTNSNQQSLNFNYNSEQRPKKTLVN
                     VRVGGGTNLGFDFLGRSQYFGAYLETTMNFQRQTYFGFGYERGYERIFEEEFGPKRSA
                     THPGTFSGTDPERAAHRNSGYVYAGTIPSRKYNFSTSITYDAGILDFDFGGGPRFPRV
                     SPGALLDPNAPLDPGAGKGVNLEGRTELQPTSAFNVILNYNYNRLIRDDTRQAAFRSH
                     IFSLRVKYQLSRFVFARIRLDYNTLAANLNEQILFGWTPNPGTALYVGYNDNLNRNGF
                     NPFDGQFEPGFQRNRRSFFVKISYLFRRSLK"
BASE COUNT          545 a          551 c          518 g          439 t
ORIGIN      
        1 accgccaaac cagcgagaag cggcaaaaca tacgccacga caatgagtgg caaggcgatg
       61 gtaagggcgc gtggataggc acgctgtgga tgatcaacct ctccggcata ggttgaaaca
      121 ttatcccagc cacagtaatt ccacagtaca actgaaaatc caatgccaat ggtctttaaa
      181 tccactggtt gcgaccaccc gcttttgatt gccgcccagg cggttcccgt cgcgccagat
      241 cgagaaagac tgagaacggt caacaatcca aagggaatca gtaccagcaa gatactcatg
      301 agtgcattgt tgccgaccgc acgcgcgcca agcaggttga cacccagcgc acataagaca
      361 accaccagcg caattcccca gcgaagccaa aaaactgacc aggtacaatc cccatgttca
      421 tccagggcca gaactggcag aaaaaatgcc agataattca caaacaaaac gggataaatc
      481 gccatgtcca cggcggtata acaaatggtc caccagcctt cctggacacc ccaaaagtcg
      541 cctaaagcgg ttcggaccca gtggtaatac ccaccttcca ccggcatcgc gcttgccagt
      601 tcggcgacca tgagcgccgt cggcagcccc cacaacaacg gtgtgatcag gactaacacc
      661 attgcccatc ccggattcat ttcctgcacc agtgattcaa ggccgtatgc accgcctgaa
      721 accgtgaaaa aaatcaagcc caccaactga agcgtggtga gctttcgcgg gtgttgtgat
      781 tcagttgtca ttttttcgcg ttcttgacta cctcctgcca gattggaatg aattttaagg
      841 cgggcaagaa tacaggaacc gatgagtcag taccacccgc gtcagtgggt gggccggttt
      901 gctttcaatc ccacccactg acgcgggtgg tactgacaaa cccttagaaa ccggattact
      961 tgaggctgcg gcgaaacagg taggaaattt tcacaaaaaa tgaacgccga ttgcgctgga
     1021 atcccggctc aaactggccg tcaaacggat tgaagccatt acgattgagg ttgtcgttgt
     1081 atccaacata gagcgccgtg ccagggttgg gcgtccagcc aaacagaatt tgctcgttga
     1141 gattggcggc cagggtgttg taatcaagcc gaattctggc aaagacaaaa cgagatagct
     1201 ggtatttcac ccggagcgaa aaaatgtgag accgaaaggc cgcctgccgg gtgtcatcgc
     1261 ggatcaaccg gttgtaattg taattgagaa tcacgttgaa agctgaggtc ggctgcaact
     1321 cggttcggcc ttcaaggttc actcccttgc cagcaccagg atcaagcggc gcattggggt
     1381 caagcagggc acctgggctg actcgtggaa accgagggcc gccgccaaaa tcaaaatcca
     1441 ggattccagc atcataggtg attgacgtgc tgaagttgta ctttcggctc ggaattgtac
     1501 cggcatagac ataccctgaa tttcgatgag cggcgcgttc cgggtctgtt cccgaaaaag
     1561 tacccggatg cgtggcggat cgcttggggc caaattcttc ttcaaaaatt cgctcgtacc
     1621 cgcgctcgta tccaaatcca aaataggtct ggcgttgaaa attcatcgtt gtttccaggt
     1681 aggccccaaa gtattgagac cggcccagga aatcaaaccc taagtttgtt ccacctccca
     1741 cccgaacatt gaccagcgtt ttcttggggc gttgttcgct gttgtaatta aagttgagcg
     1801 attgttgatt ggaattggtt cgccgggtaa atccgacatt ggttcgatac tcctttgacc
     1861 ggccctgggc ccggtagtca acggtgaggt atcgcccttt tttcgagagt ccaatcgaat
     1921 agccaattcc gtttcccgtc cggtaaatgg tgcttccctg gtcagcatca aaaaaatact
     1981 ggcgggaggg cgttcctaaa acctgaaaat tgaaaaaaaa ctgctgtcca atgcggaact
     2041 tcccatcaaa ccc
//
LOCUS       DAOQDO010001211         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53369_length_2051_cov_2.540080, whole genome shotgun sequence.
ACCESSION   DAOQDO010001211 DAOQDO010000000
VERSION     DAOQDO010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53369_length_2051_cov_2.540080"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1274
                     /locus_tag="PKZ53_17375"
     CDS             144..1274
                     /locus_tag="PKZ53_17375"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ42262.1"
                     /translation="MANDISQSDQKRNQKFVWLMVFGFFTINFLLLFSYRYLDDLTRF
                     KSGTFLTRLIEEMTGAYTAMLLVPIVFWSSRQFRLYRPHLLKSLAAHLGTVLFQSFLH
                     TSLMAVTRQAIFFLFGLGVYDYGYMPLRYLMEFPNQLLWYSAVVGFIHLFDRYQELRD
                     QELRASRLESQLAQSRLQALQLQLQPHFLFNTLNTISAVLYEDIEKADRMIARLSDFL
                     RATLKTASSQQISLGDEIEFLNLYLEIMQARFEEQLAVTLEIEAEARPALVPRLVLQP
                     LVENALKHGVDQTTGKIRIDVSAKINGAHLELEVSDQGPGLNGNGLASESTKVGLANL
                     RERLQVLYPEHHTLAVHNGDHAGLTVHMTLPLQTSLQEKVPA"
     gene            1271..>2051
                     /locus_tag="PKZ53_17380"
     CDS             1271..>2051
                     /locus_tag="PKZ53_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ42263.1"
                     /translation="MTLIRTLIVDDEGPARRKIRRLLETDPEIEVIGEAATGPEALAQ
                     IRSLKPGLVFLDIQMPGMNGLELAKAIAGEPNPVVVFITAFDQHALRAFEVQAVDYLL
                     KPFDPDRFRQALARAKEQVKLRTNRDLSHNLQSLLAQLQPTPPVFPDRILIELDERAF
                     FLAVEKIHRVEAAKNYINLHTQTETHTLRSTLESFALKLNPQKFVQVNRSSIVNLDRI
                     RELQPWFHGEYRIILTDGTELMWTRRYVGRAAELLNRNRMKN"
BASE COUNT          496 a          565 c          496 g          494 t
ORIGIN      
        1 gaattcacca atcctccacc cttccttcga gcaatcgcca acctttaacc cgtaaggttg
       61 ggccaatggt agtaaattac tccgtaattt tattttgtca acctttttat tttgggaagg
      121 cggaaaagaa agagagcacc tgtatggcaa atgatataag ccagtcagac cagaagcgca
      181 accagaaatt tgtctggctg atggtgtttg gcttctttac catcaacttt ttgttattgt
      241 tttcctatcg ctatctggat gatttaacac ggtttaaatc cggcacattt ctcacccggc
      301 tgattgaaga aatgacgggt gcctatacgg ccatgttgtt ggttccaatc gtgttttggt
      361 cgtcacgcca gtttcgactc tatcgcccgc acctgctcaa atcactggcg gctcaccttg
      421 gtacggtgct atttcaatca tttttgcata cgtcgttgat ggccgtgacc cggcaggcta
      481 ttttttttct gtttgggctt ggggtttacg attacggata tatgccgttg cgctatctga
      541 tggaatttcc caaccagttg ctctggtatt cagccgtggt tggatttatt cacctctttg
      601 atcgctatca ggaattgcgc gaccaggaac tgcgggcctc acgacttgaa tcacaactcg
      661 ctcaatcccg gctgcaggca ctgcaactgc aactccaacc gcattttctg tttaacacgc
      721 tcaacaccat ctcagccgtg ctctatgaag acatcgaaaa agctgaccgg atgattgccc
      781 ggctgagtga tttcctccga gccaccctca aaactgccag ttcccagcaa atttcactcg
      841 gcgacgaaat tgaattcctg aacctttacc ttgaaattat gcaggcccgg tttgaagaac
      901 aactcgccgt cacgctggaa attgaagccg aagcccgacc ggcgctggtg ccccggctgg
      961 tcctgcagcc actggtcgaa aatgcactca aacacggcgt tgaccagacc acgggaaaga
     1021 ttcggattga tgtttcggcc aaaattaacg gggcgcatct ggaacttgaa gtcagcgacc
     1081 aggggccagg cttgaatgga aatggattgg cgtcggaatc aaccaaagtt gggctggcca
     1141 atttgcgcga acgcctgcaa gtgctctatc ccgaacatca tacgctggcg gttcacaatg
     1201 gtgaccacgc cgggctgacc gttcatatga ccttgccgtt gcaaacttcg ctccaggaga
     1261 aagtaccggc atgacgctga ttcgaacgct gattgtggat gacgaaggtc cagcccgtcg
     1321 caaaatccgc cggttgctcg aaactgaccc ggaaattgaa gtgattggcg aagcggccac
     1381 cggcccagaa gcgctggcac agattcgaag cctcaagccg gggctggtct ttttggatat
     1441 tcaaatgcca ggaatgaatg gacttgagct ggcaaaagcc attgcaggag aaccaaaccc
     1501 ggtggtggtg tttatcaccg cgtttgacca gcatgcgctc cgggcgtttg aagtccaggc
     1561 cgtggactac ctgctcaaac cctttgatcc ggatcggttc cggcaggcgc ttgcgcgagc
     1621 caaagaacag gtcaagcttc gcaccaatcg tgatttgagc cacaatctgc aatcgctgct
     1681 ggcgcaatta cagccgaccc cgcccgtttt tccagaccgc atcctgattg aactcgacga
     1741 acgggctttt tttctggcag ttgaaaaaat ccaccgggtt gaagccgcca aaaattacat
     1801 caatctgcat acccaaaccg aaacccacac cttgcgcagc accctcgaaa gctttgccct
     1861 caaactcaac ccgcaaaaat ttgtccaggt caatcgctca agcattgtca acctggaccg
     1921 catccgtgaa ctccagccct ggtttcacgg cgaatatcgc atcatcctca ccgatggcac
     1981 cgaattgatg tggacccggc gctacgttgg ccgcgcagcc gaactcctca accggaacag
     2041 aatgaagaat g
//
LOCUS       DAOQDO010001212         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53374_length_2051_cov_2.309619, whole genome shotgun sequence.
ACCESSION   DAOQDO010001212 DAOQDO010000000
VERSION     DAOQDO010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53374_length_2051_cov_2.309619"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..703)
                     /locus_tag="PKZ53_17385"
     CDS             complement(89..703)
                     /locus_tag="PKZ53_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42264.1"
                     /translation="MIKSKHSITARLGCRERAEKILPEVTEARALVQLREMKQAFARY
                     IGKTYGHLAVDCATCQTPCCADAEFVNVNITRLEAVAMLRTIEASSNHGPEKVAEVLR
                     RAAASAQEYGLTEDGDTFEKTYACPLFEPGKGCLVHWKAKPAPCIQHGCYERWQDLPE
                     TRSMRRVEHSVAKLNEQVYDEPVEYQTIPIWLLRIAKETAENEE"
     gene            complement(830..>2051)
                     /locus_tag="PKZ53_17390"
     CDS             complement(830..>2051)
                     /locus_tag="PKZ53_17390"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42265.1"
                     /translation="VEFALAPEKSFVWLVSQKSIEVFPLPPKEEIERCASRVYELLIV
                     RNLPVTTTQERKIQLSDGDFSQTGRKAQIAKADMAFPAAVRELSQLLFGQFANKLTKK
                     RLLIVPDGRLQSIPFGALPLPSEPTKSATAQPFLIEQHELVVLPSATTLLVKRQQPAA
                     PLQPQSKKVLVVADPVFDLQDSRIAEISKHRPASEAMVAEARNLSLLPAPTGNAIQRL
                     VFTRQEAAQISRLLKPDEGKQLLDFDASRQSVLAENLNDYRMLHFATHGVFNRQTPEL
                     SGLILSQVNRNGEPENGFLSLTDVFNLRLNADLVVLSACQTALGKDYWGEGMVGLTRG
                     FLYAGTNRVVSSLWMVDDSATSELMAEFYRRMLGKDRLSPAAALRAAQLKMLSRPNRK
                     HPFYWAAFQVQGEF"
BASE COUNT          493 a          508 c          564 g          486 t
ORIGIN      
        1 aagaacgaag aacgaagaac gaagaacgaa gaacgaagaa cgaagaacga agaaccagga
       61 acgaagaacc aggaaccaac cacattcttc attcttcatt ctcggcagtt tctttcgcta
      121 ttcgaagcag ccagatcgga atggtttgat attcgacagg ttcatcatag acctgttcgt
      181 tcaatttggc gacagagtgc tcaacgcgac gcatcgaacg agtttcgggc agatcctgcc
      241 agcgttcata gcatccgtgt tgaatgcacg gcgccggctt ggctttccag tggaccaggc
      301 agccctttcc aggttcaaac agcggacagg cgtaggtttt ttcaaaggta tcgccgtcct
      361 cagtcagccc gtattcctgg gccgatgctg ctgcccggcg gagcacttcc gccactttct
      421 cagggccatg attggaactg gcttcaatgg tgcgcaacat cgcaacggct tcaaggcgcg
      481 tgatgttgac attgacaaat tcggcatccg cacagcaagg cgtctggcag gtggcgcaat
      541 caaccgccag atggccatac gttttgccaa tgtagcgggc aaacgcctgt ttcatctcgc
      601 gaagttgcac cagggctctg gcttcggtga cttcgggcag tattttttca gcacgttccc
      661 ggcatcccaa acgtgccgtg atggagtgtt tcgattttat catcggacaa aactatgcag
      721 acagtggctg gatgtgatga gttggaaccc taaacttagc aaatcctgag tgaagagagg
      781 aatagcaaag agagtgaaat cgcgaagtcg cacaaccaga gcgagtcgct cagaactcgc
      841 cctgaacctg aaacgcagcc cagtaaaatg gatgtttgcg attggggcga ctgagcattt
      901 tcagttgggc agcccggagc gctgcggctg gggacaggcg gtctttgccg agcatgcggc
      961 ggtaaaactc agccatcaac tcgctggtgg cactatcgtc aaccatccac agactcgaca
     1021 ccacccgatt ggttccggca tagagaaaac cacgcgtcaa cccgaccatg ccttctcccc
     1081 aatagtcttt acccagagct gtttgacagg cgctgagcac caccagatcg gcattgagcc
     1141 ggagattaaa cacgtctgtc aggctcaaaa agccattttc aggctctccg tttcggttga
     1201 cctgactcaa aatgaggccg gaaagctctg gggtctggcg gttgaaaaca ccgtgggtcg
     1261 caaaatgcag catgcgatag tcgttgagat tttcggcgag tacgctttgg cgactggcat
     1321 caaaatcaag cagttgtttt ccttcatcgg gtttgagcaa tcgcgagatt tgcgcggctt
     1381 cctgacgggt aaacaccagc cgttggatgg cattgccagt tggggcgggg agtaagctca
     1441 ggtttcgagc ctcagccacc attgcctctg aagctggtct atgtttgctg atttcagcaa
     1501 ttcttgaatc ctgaagatca aaaaccggat ctgctacaac aagtactttt ttggattgag
     1561 gttggagtgg agcggctggt tgttgacgtt tgaccagcag cgttgtggct gaaggcagga
     1621 ccaccagttc gtgctgttca atcaaaaagg gttgggcggt tgccgatttg gtcggttccg
     1681 atggcagtgg taaggctcca aatggaatgg attgcagacg gccatctgga acaatcagca
     1741 atcgtttttt ggtgagcttg ttggcaaact ggccaaataa aagctggctt aattcgcgaa
     1801 ctgcggctgg aaaagccata tcggctttgg caatttgggc tttgcgccct gtttgtgaaa
     1861 agtctccgtc gctcaattga attttgcgct cttgggtcgt ggtcaccggg agattgcgca
     1921 cgatcagtaa ttcatacacc cggctggcac agcgttcgat ttcctctttc ggcggcagtg
     1981 ggaaaacttc aatcgatttt tgtgacacga gccagacaaa gcttttttca ggtgccaggg
     2041 caaattccac c
//
LOCUS       DAOQDO010001213         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53465_length_2049_cov_4.628385, whole genome shotgun sequence.
ACCESSION   DAOQDO010001213 DAOQDO010000000
VERSION     DAOQDO010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53465_length_2049_cov_4.628385"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKZ53_17395"
     CDS             <1..533
                     /locus_tag="PKZ53_17395"
                     /inference="COORDINATES: protein motif:HMM:NF021831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HNJ42266.1"
                     /translation="GPAVNLTLLKGGLSQQTIASFISVGSNGSGSYAWAIPSTLTAGT
                     DYTIRITSTESTAIADVSNNSFTIVNGSTPGVVTIQVVTPNGGETWKTRTRQAITWSY
                     TGNPGTKVKIELYKGGAFKQVISAGATLGSNGAGAFNWLLPSTLAAGTDYKIKITSTT
                     STAITDLSDQNFSITR"
     gene            complement(669..1307)
                     /gene="tmpT"
                     /locus_tag="PKZ53_17400"
     CDS             complement(669..1307)
                     /gene="tmpT"
                     /locus_tag="PKZ53_17400"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNJ42267.1"
                     /translation="METTYWHQKWEKNDIAFHTETANPLLVQYFDELSLAKGSRVFLP
                     LCGKTLDIAWLLSQGYCVAGAELSKLAIEQLFAGLGVEPELSRIGEMDHYRAQNIDIF
                     GGDIFELSSQIPGPVDAIYDRAALVALPEEIRKRYTAHILEITDKAPQLLICYEYDQS
                     VMPGPPFSISDKEVHQHYQDTYEITFLARTDVPGGLKGICAAQEKVWLLSRA"
     gene            1792..>2049
                     /locus_tag="PKZ53_17405"
     CDS             1792..>2049
                     /locus_tag="PKZ53_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42268.1"
                     /translation="MQTGFNTNRISFPIFSTFNYWLIIAGLLVINGFSSGISAQQASP
                     ALFSRAEPNQDVRAFLKSEDPAQRLSINGDTLLRLQSKSVQV"
BASE COUNT          531 a          489 c          466 g          563 t
ORIGIN      
        1 ctggccctgc tgtgaacctg acgttgctca aaggtggcct gtcacagcaa actattgcat
       61 cgtttatttc ggttggaagc aatgggagcg gatcgtatgc ctgggcaatt ccttcaactc
      121 tgactgctgg aactgattac acgattcgaa tcacaagtac tgagagcact gccattgctg
      181 atgtcagcaa caactcattt actattgtta atggatcaac cccaggtgtg gtaaccatcc
      241 aggtggtgac acccaatggt ggagaaacgt ggaaaacgcg aacgcgccag gccattacct
      301 ggagttacac tggaaatcca ggcacaaaag tcaagatcga actctataaa ggcggtgcct
      361 tcaaacaggt tattagtgcg ggtgccacgc ttgggagtaa cggcgccggg gccttcaact
      421 ggctcttgcc ctcaacactg gcggctggga cggattataa aatcaagatc acgagtacga
      481 ccagcaccgc catcactgat ctgagcgatc aaaacttcag cattacgcgg taggaaaaat
      541 gctgggtttc cgcccggtaa gaccaggtga gcaagaaact atttcttgaa gcagtcgcaa
      601 gccgttacaa gacccatcgc ctggggtgca tcgccacccc aggcgattct tttccttaat
      661 cctggtgttt atgcccttga aagcaaccaa accttttctt gtgcggcgca gatacctttc
      721 aggccaccag gaacatctgt tcgggcaagg aaagttattt cataagtgtc ttgatagtgt
      781 tgatgtactt ctttatcact gattgaaaac ggaggaccgg gcattacgct ttggtcatac
      841 tcataacaaa tgagcagttg aggcgcttta tccgtgatct ccaggatgtg cgccgtgtag
      901 cgctttcgaa tttcttcagg gagcgccacc aaagctgccc ggtcataaat tgcatccacc
      961 gggccaggta tctggctgga caactcaaaa atatcgccac cgaagatgtc aatattttgg
     1021 gcgcgatagt ggtccatttc gccaattctc gatagttctg gttcgactcc aagcccggca
     1081 aataattgct caatggccag tttgctcaac tcggcgccag caacgcaata cccttgagac
     1141 aacagccagg caatatcgag cgtcttgccg cacagtggga gaaagacgcg gctgcctttc
     1201 gccaaagaaa gctcgtcaaa atattgcacc agcagcgggt tggctgtctc tgtgtggaat
     1261 gcgatatcat ttttttccca cttttgatgc cagtacgtcg tttccatatt ccatcactcc
     1321 gttcgtatgc gaatttaaaa attaagtgac tcacgttata gcctcttacc tgacagagcg
     1381 gaagacgcac cacttacatt ttattcgtgc atcaaacgcc acatcataga tcgccattca
     1441 gataaatatt tctttggagt gctgcaactt gttgcagctt tgaaaagcgg tgacaagtcc
     1501 ccgcactcca aagaatttct attttatttt actttcgttt tatttcaatt tgataggaaa
     1561 tcacttttgt gaattcaatg taaataatac tggactgtag tcagtggatt acagcctgcg
     1621 atgtgttaac ttttttaaat aaatgtgtta gctgttcacc ggcgcaggat gcagtaattc
     1681 tttgtttgga aaataaagaa ttgtcggagt tttttctttc gttactttgt acggcatatc
     1741 gcttgccatc cagggagtca gtttaactta tgactacttt tggagggtac aatgcagacc
     1801 ggatttaata caaaccgaat ttcgtttcca atattttcaa cattcaatta ctggttgatt
     1861 atagctggct tactcgtgat caatggcttt tcatctggga tctctgccca acaggcttca
     1921 ccagcactct tttcacgggc tgagccaaat caggatgtac gcgcttttct aaagagtgaa
     1981 gatccggctc aacggctttc catcaacggt gacaccctgt tacgcctgca atcaaagtca
     2041 gtacaggtt
//
LOCUS       DAOQDO010001214         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53480_length_2049_cov_3.008526, whole genome shotgun sequence.
ACCESSION   DAOQDO010001214 DAOQDO010000000
VERSION     DAOQDO010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53480_length_2049_cov_3.008526"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PKZ53_17410"
     CDS             complement(<1..990)
                     /locus_tag="PKZ53_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNJ42269.1"
                     /translation="MNCFRSVAPRLLGVWLLVLWSASLSVLAQETFDVKAHYTKYEYQ
                     IPMRDGVKLFTSVYVPKDTSQKYPILMNRTPYTVSPYGADYKENIGPSVAMAKEGYIF
                     VYQDVRGKWMSEGDFMDVRPVNPNKKGPQDIDESSDTYDTIDWLVKNIPNNNGKVGMW
                     GISYPGFYAALGLVESHPALVAVSPQAPIADWFIGDDFHHNGAFYLIDFFRFYYAFGQ
                     PRPKPTIERSPNFEYGTPDGYKFFLELGPLTNINAKYYKNKVAYWNEASQHGTYDEYW
                     KARNVLPHLKNVKPAVMTVGGWFDAEDLYGPLHIYDAVEKNNPGRFNMLVMGPW"
     gene            1158..1991
                     /locus_tag="PKZ53_17415"
     CDS             1158..1991
                     /locus_tag="PKZ53_17415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ42270.1"
                     /translation="MQESNGLAITHCTTTNLRRWNELVAIHAQSKFYDLEGFKAGNTS
                     LRPLEIEEVGDVEGKTLLHLQCHFGMDTLSWARRGAQVTGVDFSDKAIELARSLSAEL
                     ELNARFICSNVYDVPAVLNEQFDVVFTSYGVLVWLHDYDRWAQIVAGALKPGGTFYIA
                     EIHPFPYVFDEASTTDAFRLKYPYFWAPEPVECLTEGTYTDRTAEIQETTEYCWIYNL
                     GDIVTALIKVGLHIEFLHEFPFCVYQAFPFMQQSEDGWWRMPDHQTSLPFLFSIRARK
                     P"
BASE COUNT          503 a          510 c          505 g          531 t
ORIGIN      
        1 ccagggcccc atcaccagca tattgaaccg gcccggattg tttttctcca cggcatcata
       61 aatatggagc gggccataca aatcttcggc atcaaaccag ccaccaaccg tcattaccgc
      121 tggtttgaca tttttcaaat gcggcaggac gtttcgggct ttccagtact cgtcataggt
      181 gccatgctgg gatgcttcgt tccagtaggc aaccttgttt ttatagtact ttgcattgat
      241 gttggtcagt ggtccaagct ccaggaagaa cttgtagcca tcgggtgttc catactcgaa
      301 attgggtgac cgttcaatgg ttggtttggg gcgtggttga ccaaacgcat aataaaaccg
      361 aaagaaatca atcagataaa aagcgccatt gtggtggaaa tcatccccga taaaccagtc
      421 agcaatcggg gcttgcggtg aaacagccac cagtgccggg tgagattcaa ccagcccgag
      481 tgccgcgtaa aatccagggt aggaaatgcc ccacatccca actttgccgt tattgtttgg
      541 aatgtttttc accagccagt caatcgtgtc ataggtatcg gagctttcat caatatcctg
      601 tggccctttt ttattcgggt tgacgggtcg cacgtccata aagtcgcctt ctgacatcca
      661 ttttccacgc acatcctggt acacaaagat ataaccttct tttgccatcg ccacggatgg
      721 tccgatgttc tctttgtagt ctgcaccgta aggcgacacg gtatagggcg ttcggttcat
      781 caaaattgga tatttttggg atgtgtcttt ggggacatac accgaggtga acagcttcac
      841 cccatcacgc attggaatct gatattcata ctttgtatag tgggctttga cgtcaaaggt
      901 ctcttgcgcc agcaccgaga gactggcact ccacaggacc aggagccaca cacccaacaa
      961 acgcggggca acagatcgga aacaattcat cgcaacagag tcctcctcac acttcaggaa
     1021 aagttaattt tggaaatcaa tgtaggggta ataccaatta gggttcagag ttcagggttc
     1081 agagttcagg gaaatacaat tttttcaata ctttagcctt cccgtaatac gaggatttca
     1141 ttgcaaaatg gggtaatatg caagaatcaa acggtttggc catcacgcac tgtaccacaa
     1201 ccaacctgcg gcggtggaat gaacttgtcg ccatccatgc ccaatcaaag ttctatgatc
     1261 ttgaagggtt taaggccgga aacacctcgt tgcgtccgct tgaaatcgag gaagtcggag
     1321 acgttgaagg gaaaacgctc ctccatctgc aatgccattt tggaatggat accctgtcct
     1381 gggcccgacg tggggctcag gtaacgggcg tggatttttc agacaaagca attgaactgg
     1441 ccagatcact gagtgccgaa cttgaactca atgcccggtt tatctgttcc aatgtctatg
     1501 atgtaccggc agtactcaac gaacaattcg atgtggtatt tacgtcctac ggcgtgctgg
     1561 tgtggttgca tgattatgat cgctgggccc agatcgtggc tggggcgtta aaacccggcg
     1621 gaacctttta tatcgctgaa atccatccgt ttccctatgt gtttgacgaa gcttcaacca
     1681 ccgatgcgtt ccggctgaaa tatccttatt tttgggcgcc cgagccagtc gagtgcctga
     1741 ctgagggtac ctacactgac cgcacggctg aaattcagga aactaccgaa tactgctgga
     1801 tttacaattt aggcgacatc gtcaccgcac tcatcaaagt cggattacac atcgagttcc
     1861 tgcacgagtt tccattctgc gtctatcagg catttccctt tatgcagcaa tcagaagacg
     1921 gctggtggcg aatgcccgac catcagacct cgctgccatt tttattctcg attcgggcac
     1981 ggaaaccgtg aaaattgggg ttccgggttc cgggttccgg gttccgggtt ccgggttccg
     2041 ggttccggg
//
LOCUS       DAOQDO010001215         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53484_length_2049_cov_2.814945, whole genome shotgun sequence.
ACCESSION   DAOQDO010001215 DAOQDO010000000
VERSION     DAOQDO010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53484_length_2049_cov_2.814945"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1023)
                     /gene="fmt"
                     /locus_tag="PKZ53_17420"
     CDS             complement(79..1023)
                     /gene="fmt"
                     /locus_tag="PKZ53_17420"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNJ42271.1"
                     /translation="MRIIFMGTPAMALPTLDQLIADKHEIVGVFTQPDRPAGRGNKIT
                     APPVKELALEHNLPVFQPTKIKTPEMHEHFKSLDADLAVVVAYGRILPQHLLDTPRLG
                     CWNVHFSLLPKYRGAAPVNWAIANNEPLTGVTVMQMDAGLDTGDILLQTECWIAREDT
                     TVTLGERLSEIGAQSLAEALHKHAEGTLPRIPQDHSQATFAPILNREDGLIDWSLPAE
                     TISCRLRGFQPWPGVYTLLNGTRTVIWKATPGTHKGAATAQPGAVLGLTPANQLAVAC
                     GGDSALLVEELQSEGRKRLPSRDFANGMRLAAGTVFGT"
     gene            complement(1096..>2049)
                     /locus_tag="PKZ53_17425"
     CDS             complement(1096..>2049)
                     /locus_tag="PKZ53_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42272.1"
                     /translation="QGKELQLKISPPGDHQVKVIQGTRAIVAAKLYKVDAKIQKVEQV
                     EPLKALLIVETLPKASVYADDVFCGETSEDGTLTIDKLSPSEPHRIRIEANRYRTWES
                     DVEVTTEEDTKLPIKLDAIIEYADNFFDLNKWDAPADWKAETQLLKVRGAQTIGMPKE
                     VGFRNCEVAFDLQLVKGGRASWVIHAKDTKKDYYLFSLELAGGAAVLKTFVCRDGIPG
                     TPVQTDFIPVPVAEKKWHHFRILVEGNKISHLVTPNESPDEYSVSLFQDRDNTFPYGS
                     IGFATLQGEEFWVGAFVVCPEGAGCRPEMKAAPVDSLRKQK"
BASE COUNT          446 a          520 c          556 g          527 t
ORIGIN      
        1 cagccctggt ttcttcagcc ccaagccctc agccctggtt tcttcagccc caagccctca
       61 gccctggttt tttcagccct aagtaccaaa aaccgttccc gccgccagtc gcatgccgtt
      121 ggcaaagtca cgggacggaa gtcgtttgcg gccttcggat tggagttctt caacgagtaa
      181 ggccgagtca ccaccacagg caacggcaag ctggttggcc ggagtcagac ccagcaccgc
      241 tccgggttgg gctgtggcgg cacctttgtg tgttccgggt gttgctttcc aaatgacggt
      301 gcgcgttcca ttgagcaaag tatagacacc tggccagggt tgaaagccac gtagccggca
      361 cgaaatcgtt tcagccggaa gcgaccagtc aatcaggccg tcttcacgat tcagaatcgg
      421 agcaaacgtc gcctggctgt ggtcctgtgg aatgcgaggc aatgtgcctt cagcatgttt
      481 gtggagcgct tcggccagag attgcgcccc aatttctgac aggcgttcac cgagcgttac
      541 ggttgtgtct tcgcgggcaa tccagcattc ggtctggagc aaaatatccc ctgtgtcaag
      601 accggcatcc atttgcatca cggtcacgcc agtcaagggt tcgttattgg caattgccca
      661 gttgacgggt gccgcgccgc gatatttcgg aagcagcgag aaatgcacat tccagcagcc
      721 aagtctcggt gtatcaagca gatgctgagg caaaatccgc ccatacgcga cgactaccgc
      781 cagatcggcg tcaaggcttt tgaaatgctc gtgcatttcc ggggtcttga ttttagtcgg
      841 ttgaaagacc ggcaggttgt gttccagcgc cagttctttg accggaggcg cggtgatttt
      901 gtttccgcgc ccggctggac gatcaggttg cgtaaaaacg ccgacgattt cgtgtttatc
      961 ggcaatcaac tggtcaagcg taggaagggc cattgccggg gtgcccataa aaataattcg
     1021 cataattgta aatgaagggc gcttcggacc gcgcccttga agtttgaaga gtagattgtg
     1081 gaggttaaga ggcggttatt tctgcttgcg taaggaatca accggagcgg ctttcatttc
     1141 gggacggcat ccggcacctt cgggacagac cacaaaggcg ccaacccaga attcttcgcc
     1201 ctggagcgtc gcaaatccaa tgctgccata gggaaacgtg ttgtcacgat cctggaagag
     1261 gctcacggaa tattcatccg ggctttcatt gggcgtgacc agatggctga ttttgttgcc
     1321 ttcgaccagg atgcggaagt gatgccattt cttttcggcc actggcaccg ggataaaatc
     1381 agtttggact ggtgttccgg gaatgccatc gcggcagaca aatgtcttga gcacggcggc
     1441 tccgcctgcc agttcaagcg aaaacaggta gtaatctttc ttggtatctt tggcgtgaat
     1501 gacccacgaa gcgcgtccgc ctttgaccag ttggagatca aacgccactt cgcaattgcg
     1561 gaaaccgact tcctttggca ttccgatggt ctgggcacca cgaactttta agagttgtgt
     1621 ttcagctttc caatccgccg gcgcatccca tttgttcaag tcaaagaagt tatcggcgta
     1681 ttcgatgatg gcatcgagtt tgattggaag cttggtatct tcttctgttg tgacttcaac
     1741 atcagattcc caggtacgat agcgattggc ttcaatgcga atgcggtgtg gttcacttgg
     1801 tgaaagcttg tcgatggtta acgttccatc ttctgaggtt tcgccgcaaa acacgtcatc
     1861 tgcgtaaaca cttgcctttg gaagtgtttc aacaatcaat aaagctttta aaggttcgac
     1921 ctgttcgacc ttttggattt tggcatcaac tttgtaaagt ttagccgcaa caattgctct
     1981 tgtgccttga ataactttaa cttgatgatc tcctggcgga gagattttga gttgcagttc
     2041 tttgccttg
//
LOCUS       DAOQDO010001216         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53492_length_2049_cov_2.635908, whole genome shotgun sequence.
ACCESSION   DAOQDO010001216 DAOQDO010000000
VERSION     DAOQDO010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53492_length_2049_cov_2.635908"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1518
                     /locus_tag="PKZ53_17430"
     CDS             85..1518
                     /locus_tag="PKZ53_17430"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNJ42273.1"
                     /translation="MTTRRQFLKLFGFAALGVGASGCQVAEKLAPLVGIGRDFTAQLP
                     IWTGSAAEIKALNFVNRITWGARPKDVPTVVQLGIDAFLEQQLAPEGIPDDKAHWLTR
                     RIETLNLGVAGVYNFTAEELLLDLRRATVLRAIYSERQLLERMVEFWSDHFNIYGEKE
                     SCGQLKVVDDREVIRRHALGNFRDLLRASATSPAMLVYLDGNANVAAHPNENYARELL
                     ELHTLGVDGGYSQHDVQEAARALTGWKVRDRFRRGQVVLNTESHDSQPKRVLGQLLEG
                     EATSELEQLLDVVCRHPSTATFLAKKLCRRFVSDQPSNQLVSQVARVFTESQGDIRSM
                     LRVIFRSDEFRQFQPRFKRPLTYAMSALRLLNAECDGGPGIQHQLEMLGQLPFAWPTP
                     NGFPEGEGHWASSLLPRWNFACALTSGNLDGTGLDLDQIGDQVFLHVPYAIGRTLTAA
                     ESDAFSKLDAPSEQLALVLCLPEFQYQ"
     gene            1561..>2049
                     /locus_tag="PKZ53_17435"
     CDS             1561..>2049
                     /locus_tag="PKZ53_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42274.1"
                     /translation="MDHSILLLINLRGGADGLNMLIPYREDHYFRSRPTLAIPKPGEA
                     GKSALELNQQWAIHPALEPLMELYLRQDLAFVQAVGWPGTSHSHFEAWDDIEAGGLGE
                     QRPINGWLARFLQAMPETGDSALRGVALSDKVPRVLSGASGVSALQSFNEFQLATARK
                     EEM"
BASE COUNT          458 a          533 c          566 g          492 t
ORIGIN      
        1 cccgagcttg cgagtcttca gccctgagcc cgaggtcttc agctctcagt cttcggtctt
       61 cccagcccgg aaaggaaaat ggctatgaca accagacgac agttcctcaa actttttgga
      121 ttcgcggcac tcggtgtggg tgccagtggg tgtcaggttg ctgaaaaatt ggcgccgctg
      181 gtcggaattg gacgcgattt tacggctcag cttccgatct ggactggttc ggcagccgaa
      241 atcaaggcat tgaactttgt gaaccggatt acctgggggg ccagacccaa ggatgtgcca
      301 acagtcgtcc agttggggat tgatgccttt cttgagcagc aactggcacc cgagggcatt
      361 ccagatgaca aagcgcactg gctgacccgc cggattgaaa cgcttaacct cggagtggca
      421 ggtgtttata acttcacggc tgaagaattg ctgcttgatt tgcgccgggc cacagtgctc
      481 cgagccattt acagcgagcg ccagttgctg gaacgcatgg ttgagttttg gagcgatcat
      541 ttcaatatct atggcgaaaa ggaaagctgt gggcaattaa aagtcgttga tgaccgtgaa
      601 gttatccgcc ggcatgcgct tgggaatttt cgtgacttat tgagagccag cgccaccagc
      661 ccggccatgc tggtgtatct ggacggaaat gccaatgtgg cggcgcaccc caatgaaaac
      721 tatgcccgcg agttgctcga actccatacc ctcggtgttg atggtgggta ttcgcaacac
      781 gacgttcagg aagctgcccg tgccttgacc ggatggaaag tcagagatcg gtttcgtcgt
      841 ggccaggttg tgcttaacac cgaatcccat gactcgcagc ccaagcgggt actgggccag
      901 cttcttgaag gggaagccac ctccgaactg gaacaactgc tggacgtggt ttgtcgtcac
      961 ccgtccacag ccacctttct ggccaaaaaa ctctgtcgtc ggtttgtcag tgaccagcca
     1021 tccaaccaac tcgtcagtca ggtagccagg gtatttactg aatctcaagg tgatatccga
     1081 agcatgctgc gagtgatctt ccgatctgat gaatttcgtc agttccagcc caggttcaaa
     1141 cgcccattga cttatgccat gtcggcactc cgactgttga atgcggaatg tgacggagga
     1201 cctggaatcc agcatcagct tgaaatgctc gggcaattgc cttttgcctg gccaactcca
     1261 aatggattcc ctgaaggtga aggccactgg gcatccagtc ttctcccacg ctggaatttt
     1321 gcctgtgcgc tgacatcggg aaacctggat ggcacggggc ttgacctcga tcaaattggc
     1381 gatcaggtct ttttgcacgt cccctatgcc attgggcgaa cactcacggc tgccgaaagc
     1441 gatgcgtttt caaaactcga tgccccatct gaacaactgg cgttggtgtt gtgcctgcca
     1501 gaatttcaat atcagtaatc ttgtcaaagc ggcgtcaagt gggccgcact ccgaaaaatt
     1561 atggaccatt caattttgct gctcatcaat ttgcgaggtg gtgcggatgg gctcaatatg
     1621 ctcattccgt atcgtgaaga ccattacttt cggtcacgcc caaccctggc tattcccaaa
     1681 ccaggggagg ccgggaaatc agcccttgaa ttgaatcagc aatgggcgat tcatccggca
     1741 ttggaaccgc tgatggaact ctatctccgc caggatctcg cttttgtcca ggcggttggt
     1801 tggccaggaa cctcacactc acactttgaa gcctgggatg acatcgaagc cggtggtctg
     1861 ggtgagcagc gacccataaa tggctggtta gccaggtttt tacaggcaat gccagaaaca
     1921 ggcgattcgg ctttgcgcgg tgttgccttg agtgacaaag tcccacgagt tttatcgggt
     1981 gcttctgggg tttctgcgct gcaatcattc aatgagtttc aactggcaac ggcccggaaa
     2041 gaagagatg
//
LOCUS       DAOQDO010001217         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53624_length_2046_cov_3.400804, whole genome shotgun sequence.
ACCESSION   DAOQDO010001217 DAOQDO010000000
VERSION     DAOQDO010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53624_length_2046_cov_3.400804"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1929)
                     /locus_tag="PKZ53_17440"
     CDS             complement(91..1929)
                     /locus_tag="PKZ53_17440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ42275.1"
                     /translation="MPEPDPALTPPPQPEPVPVESLPVGVLQHIRPGDQLGPYTLISR
                     LGRGNFGVVWLAERRGAIATTNIALKMPLDEDVPLDSLRREASLWVRVSGHPNILPII
                     EAEVYSGQIVIASEYVSGGSLATWLQKYNGAAPTMDTALELMDGILRGLEHLHDQRVI
                     HRDLKPANIMLQHGSPRLMDFGISRVIENSLLTNTIAGTVQYMAPEAFDGFRTELTDL
                     WAAGVIFYQLVSGKLPFLQMGDIVRRDPFALPATVPDPIQHIIRRSLQKEPNFRYQSA
                     GELREVIRHLWKTASFPSSVVMSSEVLPPPDQTVILQTDDRQNTSTHSETITFSEPRP
                     ALNSVVFDRAVLDAWGNVVEWVTGECQYFDETLAEGLLLRMVSIPEGSFLMGSPSGEG
                     KSYEKPQHEVKVAPFFLGMFPVTQSQWQAVAQLPKINLELSLTPSFFQGDPMLPVEQV
                     SWEEAQEFCDRLSLANRRPFRLPTEAEWEYACRAGTSTVYAFGDNISTDFVNYDGNYP
                     HGLGNKGSHRQKTVPVGSLGVPNGFGIYDMHGNVYEWCQDIWHNSYRNSPTTAECWNQ
                     FGDDRYRVLRGGSWLNVADFCRSADRVRFAPNLKNKILGFRVALSF"
BASE COUNT          486 a          538 c          545 g          477 t
ORIGIN      
        1 gttcagggtt cagggttcag ggttcagggt tcagggttca gggttttcga atttatgtcc
       61 cttttgtccc gatgtcatcg ccaatccagc ttagaaactc aaggcaacac ggaacccaag
      121 gattttattt ttgagatttg gtgcaaaccg gacccggtcg gcgcttcggc agaaatcagc
      181 cacattgagc cacgaccctc cgcgcaaaac tcgataccga tcatccccaa attgattcca
      241 gcactcggca gttgtcggtg aattgcggta tgaattgtgc cagatgtcct ggcaccattc
      301 atacacattg ccatgcatat catagattcc aaacccgttt ggtactccga ggctaccaac
      361 gggaactgtc ttttgccggt ggctgccttt atttcccaat ccgtgggggt agttcccgtc
      421 ataattgacg aaatccgtcg agatattatc gccaaaagcg tagacggttg aggttccagc
      481 ccgacaggca tattcccact cggcttcggt cggaagccga aacggtcgtc ggttggcaag
      541 cgacagccga tcacaaaatt cctgggcttc ctcccaggaa acctgttcga ccgggagcat
      601 cgggtcacct tgaaaaaagg atggtgtcag agacaactcc agattgattt tcggcagttg
      661 ggccactgcc tgccattggg attgggtgac gggaaacatt cccagaaaaa agggtgccac
      721 ctttacttca tgctggggtt tttcatagct cttcccttca ccgcttgggc ttcccatcag
      781 gaatgagcct tccggaattg agaccatccg cagcaacagc ccctcagcca gtgtttcgtc
      841 aaaatactgg cattcgcccg ttacccactc caccacattt ccccaggcat ccagcaccgc
      901 tcgatcaaac accaccgaat tcaaggccgg tctgggttca gagaaagtaa tggtttcaga
      961 atgagttgag gtgttttggc gatcatcagt ttgcaagatg acggtctggt caggcggcgg
     1021 aagcacctcg ctgctcatca cgacactgga cgggaaagag gctgttttcc agaggtggcg
     1081 gataacctcc cgtaactcac cggcggattg ataccggaaa ttgggttctt tttgaagtga
     1141 ccgccggatg atgtgctgga ttgggtccgg aacagttgcc ggcaacgcaa acgggtcacg
     1201 ccgcacaatg tcgcccattt gtaaaaaggg caacttgccg ctgaccagtt gataaaaaat
     1261 aacaccagcg gcccacaaat cagtgagttc agttcggaat ccgtcaaagg cttcgggggc
     1321 catatattgc actgttccgg caatggtgtt ggtcaacaaa ctgttttcga tcacccgtga
     1381 aatcccaaaa tccatcaacc ggggtgatcc gtgttggagc atgatattgg ctggtttgag
     1441 atcgcgatgg atcacgcgct gatcgtgtaa atgctccaaa ccacgcaaaa taccatccat
     1501 caactctaac gcggtatcca tggtcggcgc cgccccattg tatttttgca gccaggtggc
     1561 cagtgatcca ccgctgacat attcgctggc aatcacgatt tgcccggaat agacctcggc
     1621 ttcaatgatc ggcaaaatat tgggatggcc actgactctg acccagagcg aagcctcacg
     1681 gcgcaacgaa tcgagcggga catcctcatc caggggcatt ttcaaggcaa tgttggtggt
     1741 ggcaatcgcc ccacgacgtt cagccagcca gacaacacca aaatttccac gccccagccg
     1801 ggaaatcagg gtatagggac ctaactggtc gccgggtcga atgtgctgga ggacgccgac
     1861 cggcagcgat tcaacaggaa cgggttcggg ctgcggcggc ggtgtcagtg ccgggtccgg
     1921 ttctggcata ggcgggtctt ttttgagacg tattgtgtcc atatgacagg tttcgagcgg
     1981 gaaaatccag gatacaagca acaaggttgg atgagtttgg gtttttgtca gttatcagtt
     2041 gacctg
//
LOCUS       DAOQDO010001218         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53630_length_2046_cov_3.074335, whole genome shotgun sequence.
ACCESSION   DAOQDO010001218 DAOQDO010000000
VERSION     DAOQDO010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53630_length_2046_cov_3.074335"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKZ53_17445"
     CDS             complement(<1..683)
                     /locus_tag="PKZ53_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42276.1"
                     /translation="MSQAASQATPALNKLQTFGIGLAVLGLGGLLASFAMGGPAQFFR
                     SYLIGFVYVASFPLGCLAILCIQYLSGGQWGLIIRRILEAGAKTMPLLLVLFLPMLAG
                     MTHIFAWAVPGATTNDVVLQRKAPYLNVSFFSGRAFLYLILWIGFAFALNRFARRQDE
                     YKDMRIKKFIHDFSGVGLVMLSITMTFAATDWIMSVDPHWYSTMLPVIWMAGSLLAGF
                     TTSVVVVVS"
     gene            complement(686..1387)
                     /locus_tag="PKZ53_17450"
     CDS             complement(686..1387)
                     /locus_tag="PKZ53_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ42277.1"
                     /translation="MKRLTPTTKMRSSTLVLKASCLVFLALFAAGCRQDMADLQPRYQ
                     PLQKTDFFEDHQASRLPVTGTVAYGRSFTKADPDFLREDRHLYEGKAADGLFAQTFPF
                     EITKADLDRGKQRFEAYCSMCHNYRGDGNGMIVRRGLTKPNSFHQDDVRRKPVGYYYS
                     VISNGFGSMGSYAASISVRDRWRIVAYIRALQYSQNVPLAEVPAEKRSELDKKADAKS
                     TGGHSTGGHQAGGGH"
     gene            complement(1496..2041)
                     /locus_tag="PKZ53_17455"
     CDS             complement(1496..2041)
                     /locus_tag="PKZ53_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNJ42278.1"
                     /translation="MIKEENAALYGLLAEFNTPDELVTAAKKTYDAGYRQIDAYSPYP
                     IEEAAEAIGFHKNAVPLITLCAGIMGGLTGFGMQVFMNLYHYPLNIGGRPHYSWPAFI
                     PITFELTVLFAAFTGAISMLVLNGLPMPYHPLFNVERFSRASQDKFFLCIESADPKFD
                     LAETRKFLNALNPSEVFDVEP"
BASE COUNT          525 a          564 c          520 g          437 t
ORIGIN      
        1 aacgacacga ccaccaccac agaggtagta aacccggcga gcaggcttcc tgccatccag
       61 atcaccggca gcatggttga ataccagtgc gggtcaaccg acataatcca gtcagtggcg
      121 gcaaaggtca tcgtgattga caacatcacc aacccgacgc cgctgaagtc gtggataaat
      181 ttcttgatcc gcatgtcctt gtattcatcc tggcggcgtg caaaccggtt caatgcaaaa
      241 gcaaaaccaa tccacagaat gagatacaaa aatgctcgac ctgaaaagaa gctcacattc
      301 aaatacgggg ccttgcgttg caacaccaca tcattggtgg ttgccccagg caccgcccag
      361 gcaaaaatat gggtcatccc ggccagcatc ggcaaaaaca aaaccagtaa gagcggcatg
      421 gtcttggcgc cggcttccag aatgcgccgg atgatcaacc cccactgtcc accgctcaga
      481 tattgaatac agaggatggc cagacaccca agcgggaaac tggccacata gacaaacccg
      541 atcagatagg aacggaaaaa ctgcgcgggc ccacccatcg caaaggaggc gagaagtcca
      601 cccaacccaa ggaccgccag accgattcca aaggtctgca atttgttaag tgctggagta
      661 gcctgtgatg cggcttgact catgatcaat gacctcctcc tgcctgatgg cctccggttg
      721 aatgaccacc tgtgctctta gcatctgctt tcttatccag ttcgcttcgt ttttcagcgg
      781 ggacttccgc gaggggaacg ttctggctgt attgcaatgc ccgaatgtag gccacaatcc
      841 gccaccggtc acggactgaa atcgaggcgg cataactgcc catcgaacca aacccgttgc
      901 tgatgaccga gtagtaatag cccaccggct tgcggcgaac atcgtcctga tggaacgaat
      961 tcggtttggt cagcccgcgc cgaacgatca tcccgttgcc atcaccacga tagttgtggc
     1021 acatcgagca ataggcttca aagcgctgct tccctcgatc cagatcagct ttggtgattt
     1081 caaatggaaa ggtctgggca aacaaaccat ctgccgcttt cccttcatac agatggcgat
     1141 cttcacgcag aaaatcagga tccgctttgg taaagcttcg accataggcc acggtgcccg
     1201 tgaccggcag ccgtgaagcc tgatgatctt caaagaagtc tgttttttgg agcggctgat
     1261 agcggggctg aaggtcagcc atgtcctggc gacaaccagc agcaaacaat gccaggaaca
     1321 ccaggcagct cgccttgagc acaagcgttg aagaacgcat ctttgtggtg ggtgtgagcc
     1381 tcttcattgc gtcaaaaatc ctttcgttga tgtggaggcc aattctgaat gttgggggtc
     1441 agtcccatct gtgtcagcag gtgggactga cgtcttcatt cttcattggc tctggctacg
     1501 gctcaacgtc aaatacttcg cttggattga gggcattcaa aaacttgcgg gtctcggcca
     1561 ggtcaaactt tggatcagcc gactcgatgc acaggaaaaa cttgtcctgt gacgcacgcg
     1621 aaaaccgctc aacgttaaac aatgggtgat agggcatcgg caatccattg aggaccagca
     1681 tgctgatcgc ccccgtgaac gccgcaaaca gcaccgttaa ttcaaaggta atcgggataa
     1741 atgctggcca gctatagtgc ggacgaccac cgatattgag cgggtagtgg tagagattca
     1801 taaagacctg catgccaaac ccggtcaacc cgcccattat cccggcgcat agcgtgatta
     1861 agggcaccgc atttttatga aatccaattg cttcggcagc ttcctcaatc gggtatggcg
     1921 aataggcgtc aatctgccgg taccccgcgt cataggtttt tttggccgca gtcaccagtt
     1981 catctggggt gttgaactca gccagcaagc cgtataaagc ggcattctct tccttaatca
     2041 tggtga
//
LOCUS       DAOQDO010001219         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53633_length_2046_cov_2.827725, whole genome shotgun sequence.
ACCESSION   DAOQDO010001219 DAOQDO010000000
VERSION     DAOQDO010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53633_length_2046_cov_2.827725"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKZ53_17460"
     CDS             complement(<1..396)
                     /locus_tag="PKZ53_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42279.1"
                     /translation="MGLIMITNWSPTLEPLTLAATVPQPTEASPALAKWLTVFSQSLT
                     DDLSSPGTDWRSPAQIPVQNEPEAEPQASHSDAADVDLTAGLPIPTPHQFYWSPVPVF
                     FEPPGAIPSVSGSRDAVSMNMNDLFDSGGH"
     gene            complement(524..988)
                     /gene="fliJ"
                     /locus_tag="PKZ53_17465"
     CDS             complement(524..988)
                     /gene="fliJ"
                     /locus_tag="PKZ53_17465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNJ42280.1"
                     /translation="MKRFSFSLQAVHNWRSAQLEAAERELASRTAVVTEAQAKLDGLV
                     SQQWKASETYLDVLSQPTVTPDEIALHTAYLGHLAQREQVERERLKDLATAQEAQRSV
                     TLEANRHVQVLDTLQARQRTQYLTEVKRLEQKSLDEITTMAFVRRRLESQFA"
     gene            complement(1039..>2046)
                     /locus_tag="PKZ53_17470"
     CDS             complement(1039..>2046)
                     /locus_tag="PKZ53_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNJ42281.1"
                     /translation="RVIDALGSPLDGLGPISPSDAYPLYRDCINPLARANITEPLGTG
                     VRAIDGLLTVGAGQRIGIFGGSGVGKSTLLGMMAKHTTADVNVIGLIGERGREVRGFI
                     EQELGEEGMSHSVVVVSTSDDSPLMRIRAAFAATAIAEYFKDQGAKVLLIMDSVTRCA
                     MAQREIGLAAGEPPSSKGYTPSVFALLPRLLERAGNFSNSGSITAFYTVLVDGDDLND
                     PIADTVRSILDGHIILSRTLAGRNHYPCIDVLNSASRLFREVTTPEQVRAAGRLRELM
                     AAHEKVEDLINIGAYQAGSNRLVDQAIARNDEILGFLRQSRSESVAFSSAVERLLHVG
                     R"
BASE COUNT          458 a          540 c          581 g          467 t
ORIGIN      
        1 gtgtccacct gagtcaaaca aatcattcat gttcattgaa actgcatctc gcgaacccga
       61 aaccgatggg atggcgcccg gtggctcaaa aaacacaggg actggtgacc agtaaaattg
      121 atgtggtgtg gggatgggca gcccggctgt cagatccacg tcggcggcgt ctgagtgact
      181 ggcttgaggc tcggcctcgg gctcattttg aaccgggatt tgggccggtg acctccagtc
      241 tgttccaggg gaagacaaat catcggtgag tgattgggaa aacacggtga gccatttggc
      301 cagggccggt gaggcttcgg ttggttgcgg gaccgttgcg gccagcgtca gtggttcaag
      361 tgttggtgac cagtttgtaa tcatgatcag ccccaaagta atacctgttt gcaatgaagg
      421 cagtgtatta cagaaaagtg aactcattga aaagattgta tttccctgat tcctgaccct
      481 gcccccaaaa gaggaggaga ttgtttttcc cacatcattt gggtcaggca aactggcttt
      541 ccaaccggcg gcgtacaaag gccatggttg taatttcatc cagcgatttt tgttcaagcc
      601 gttttacttc ggtcaaatat tgagttcgct gacgggcctg taaggtatca agcacctgaa
      661 cgtggcgatt ggcctcaagc gtgacactgc gttgtgcctc ttgagcggtt gccagatctt
      721 tgagtcgttc tcgttcaacc tgctcgcgtt gggcgaggtg gccgagatag gcggtatgaa
      781 gcgcgatttc atctggcgtt acggttggtt gacttaaaac gtcaagatag gtctcagacg
      841 ctttccattg ctgactgacc agaccatcca gtttggcctg agcttcggtc acaaccgcgg
      901 ttcggctggc cagttcgcgt tcggcggctt caagctgggc gctgcgccag ttatgaactg
      961 cctgtaagga aaaggaaaac cgtttcatga gatgttgtcc tccaggggtc tggggttgag
     1021 gtgcctcagt cgtgaacctc accgtcctac gtgcaagaga cgttccacag ccgaggaaaa
     1081 tgcgactgat tcgctccgac tttgccggag aaatcccaaa atttcgtcat ttctggcaat
     1141 tgcctgatca accagccgat tactgccggc ctgataggca ccgatgttga tcaggtcttc
     1201 aactttttca tgcgcggcca tcagttcgcg aaggcggcca gccgcccgga cctgttccgg
     1261 cgtggtgact tctcgaaaca gacgggaggc gctatttaac acatcaatgc agggatagtg
     1321 gttgcgtccg gccagggttc gagacaggat gatatgtcca tccaaaatcg agcgcacggt
     1381 gtcggcaatc gggtcattga gatcatcacc atcaaccagg accgtgtaaa aagcagtgat
     1441 cgagccgctg tttgaaaaat tcccggctcg ttccagaagc cgggggagca aggcaaaaac
     1501 tgacggtgtg tacccttttg acgaaggtgg ttcaccggcg gccagtccga tttcacgctg
     1561 ggccatcgcg catcgggtga ccgaatccat aatcagaaga actttggcgc cctgatcctt
     1621 aaaatattcg gcaatcgccg tcgcggcaaa ggctgcccga atgcgcatca acggtgaatc
     1681 gtcagaggtt gaaaccacca cgacggaatg gctcatgcct tcctcgccca gttcctgttc
     1741 gataaagccc cgcacctcac ggccacgttc cccgatcaat ccgatcacgt tgacatccgc
     1801 cgtggtgtgt ttggccatca tgcccagcag tgtcgatttt ccgaccccag agccaccaaa
     1861 gatgccgatg cgctgaccgg cgccgacggt taagagtccg tcaatggcac gaaccccggt
     1921 accgagcggc tccgtgatgt ttgcccgagc cagcgggttg atacagtcgc ggtacaacgg
     1981 gtaggcatct gaaggcgaaa ttggacccaa cccatccagt gggctgccaa gggcatcaat
     2041 cacccg
//
LOCUS       DAOQDO010001220         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53643_length_2046_cov_2.253641, whole genome shotgun sequence.
ACCESSION   DAOQDO010001220 DAOQDO010000000
VERSION     DAOQDO010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53643_length_2046_cov_2.253641"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2046
                     /locus_tag="PKZ53_17475"
     CDS             <1..>2046
                     /locus_tag="PKZ53_17475"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ42282.1"
                     /translation="NNGVWALAEDAEGTLWIGTNGGGLMAYRAGGEWQTYDTHQGLLS
                     QIVTTLQADPSGTLWIGTRLGVQRLSRKGTPTLETIATSDGQPLGSVYKIVTAPDGTI
                     LVASDLGLHQINPTAASPVAQRVDWVVGTVRALAFDHTGTLWVGHTGGTLMRVSGHQR
                     EVIKLSDRPFHSTITAICEDFDGNLWLATDGLGVARYHDGQLTFFSTKDGLTDNSIQS
                     MAVDHEGNLWIGAYRAGLNCLHDGKFWLYTPKEGLLDETAWQIYPDPGIGVWICTQNG
                     LNLWREERLTPFPMPERDQGAIIRAIARDQKGRLWVGTNESGVFEVKISGDKAEYIPF
                     TAHPLPNRKIRALCTDTSGTLWIGTSNGLCCLGDQGWRVYSTGDGLSVSSIMALEADP
                     DGGLWICTNGGGVNHFSNGTFQTFTSRNGMPSDVVFSTFVEKPGTVWITTNNGLVRLK
                     NNQLEVLGTKQGLWGENYYSLQRDESGNFWIGGNSGIFSVSSQELNEVADHQRERVSI
                     KLYGKADGMTNEECTGSAQACKTPDGRLWFPTLGGVVVVDPRNLPINTLKPDVRLEAV
                     LANGRSVSLQKPINLSFEDNTLEIKYTALSFVAPEKVQFQIQLAGFDPQWIDPGNRRT
                     AYYTNLPSGTYQFRVLASNNDGIWNETGATLDLSIATPLWRTWWALIGYVLALAGIIG
                     "
BASE COUNT          471 a          498 c          589 g          488 t
ORIGIN      
        1 aaacaatggc gtgtgggccc tggccgaaga tgctgagggc acgctctgga ttggcaccaa
       61 tggtggtgga ctgatggcct atcgggctgg tggagagtgg cagacttacg atacgcacca
      121 ggggttgtta tctcaaattg tgacaacgct ccaggcggat ccatctggta cgttatggat
      181 tgggactcgt ctgggggttc agcgcctttc ccgcaagggg accccgacgc ttgaaaccat
      241 tgccaccagc gatggtcagc ccctcgggag tgtttataaa attgtcacgg cgccagatgg
      301 cacaatactg gtggcatcgg atttgggtct gcatcaaatc aacccgacag cggccagtcc
      361 agtggctcag cgggttgact gggtggttgg cacggtgcgg gcactggcct ttgaccatac
      421 tggaaccctc tgggtcggtc acaccggcgg gacgctcatg cgcgtcagcg ggcaccagcg
      481 cgaagtgatc aagctctcgg atcgtccttt tcacagcacc atcaccgcga tttgtgaaga
      541 tttcgacgga aacctgtggc tggcaaccga cggtcttggc gtagctcgct atcacgatgg
      601 ccagttgaca tttttttcca ccaaagatgg cctgacggac aattcaatcc aatcaatggc
      661 ggttgaccat gaagggaatt tgtggattgg ggcgtatcgg gccggtttaa attgcttgca
      721 tgatggaaag ttctggctct ataccccaaa agaagggctc ctcgacgaaa ccgcctggca
      781 aatttatccc gatcctggga ttggtgtgtg gatctgtacc caaaatggac tcaacctgtg
      841 gcgggaagag cgattgactc catttccaat gcccgaacga gatcaggggg cgatcattcg
      901 agccattgcc cgtgatcaaa aaggccggtt atgggttggg accaatgaat ctggcgtgtt
      961 tgaagtgaaa atcagtggtg acaaagccga atatattcca tttactgccc atccactccc
     1021 aaaccgaaaa attcgggcgc tgtgtactga cacctccggt accctgtgga ttgggacttc
     1081 aaatggcctg tgttgtctgg gtgaccaggg ctggcgggtg tactcaacag gtgatggatt
     1141 gtcggtcagc tcaattatgg cactcgaagc cgaccccgat ggcggactct ggatttgcac
     1201 caatggcggt ggcgtcaatc atttttcaaa tggcacattt cagaccttta ccagccgcaa
     1261 tggcatgccc agcgatgtgg ttttttctac ctttgttgag aaacccggca ccgtttggat
     1321 tactaccaat aatgggttgg tacggttgaa aaacaatcaa ctggaagtgt tagggacgaa
     1381 acaggggttg tggggtgaaa actattattc cttgcaacgt gatgagtctg gaaacttttg
     1441 gatcggaggc aatagtggga tttttagtgt gtcaagccag gaattaaatg aagttgccga
     1501 tcatcagcgg gaacgggtgt caatcaagct ctatgggaaa gctgacggca tgaccaatga
     1561 agaatgtacc gggtcagcac aggcctgtaa aacccccgat ggccgtctct ggttcccgac
     1621 cctgggtgga gtggtggtgg ttgacccacg caaccttccg atcaataccc tcaaaccgga
     1681 cgtgcgttta gaagcggtgc tggccaatgg ccggtctgtt tcgcttcaga aaccaatcaa
     1741 cctgtcgttt gaggacaata ccctcgaaat taagtacacg gcgctgagtt ttgtcgctcc
     1801 ggaaaaagtg caattccaaa ttcaactggc tggatttgac ccgcaatgga ttgatccggg
     1861 caatcgtcga acggcctatt acaccaattt gccttcaggg acgtaccagt ttcgcgtcct
     1921 ggcttcaaac aatgatggta tctggaatga aaccggcgcg acgctcgacc tgagcattgc
     1981 cacgccgctg tggcgtacct ggtgggcctt gattgggtat gttctggccc tggctggaat
     2041 cattgg
//
LOCUS       DAOQDO010001221         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53700_length_2045_cov_2.242211, whole genome shotgun sequence.
ACCESSION   DAOQDO010001221 DAOQDO010000000
VERSION     DAOQDO010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53700_length_2045_cov_2.242211"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKZ53_17480"
     CDS             complement(<1..259)
                     /locus_tag="PKZ53_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42283.1"
                     /translation="MLKKAIKKSGRVGRKKSGVVQRCVLLYGQWTSERRIGMSHDRKR
                     LCIQGLLHDLFAARRSGVNHKIDGPALHRQAALQLQAIGGPP"
     gene            complement(373..>2045)
                     /locus_tag="PKZ53_17485"
     CDS             complement(373..>2045)
                     /locus_tag="PKZ53_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ42284.1"
                     /translation="TAEGFAILNSLSGLSQTNLSVFNQYVPVAPANDSGSITVGGRQI
                     PIGNVAIDSPAFFNRRNLVINLDYLQSDATQHRVRYIYNRERIIDTSANLPVFFSLVP
                     TDGRLFSYANTHSFSGTINNEFRFSFRRSDGPNILVPNLKFPGLDVFPNIGLLDLGLD
                     IGPNSSAPSIGKDVNYQVINNFSIVKGNHSLKLGGDVRRLIGTSFFVQRVRGDYQYSN
                     TDLFLRDISPDFLAERNVGGRPYHGNQWLAYVYAQDDWRLRQNLTVNLGVRYEYQQVP
                     FGARQQVLNAISSVPGVLEFNEPTAEKKNFAPKIGLAYSPEFKDGLLNKIFGNAGQSS
                     IRAGFSLGYDVIFDNLYTLSTTPQESATVNTQPSAAIPNYLAGGGLPSAGPTITDPTL
                     ARSITSAWIPDQKVPRAISYSLSIQRQLKDDWGLELRYLGTRGVNLFTQNRINRQPVV
                     NENRYLPTFLQMPSADQLSGLSLSLADLNALGGTESYVPRFRDAGFNGANIVVFTPNG
                     NSTYHAFSASLTKRFSHGYQLTSAYTWSHLLDDSTAELASTFTNPRRP"
BASE COUNT          472 a          545 c          549 g          479 t
ORIGIN      
        1 ggggcggccc tcctatggcc tggagctgca gggccgcctg gcgatggaga gcggggccgt
       61 cgatcttgtg attcacaccg ctccgccgcg cagcaaagag atcatgcagc agtccctgta
      121 tacagagacg ttttcgatcg tggctcatcc cgatgcgccg ctcagaggtc cattgtccat
      181 aaagcaatac acatcgttgg accacgccac tttttttcct gccgactcga ccggatttct
      241 tgatggcttt cttgagcaac acggcatccg tcgccgcgtt cggattcgga ctcctcacct
      301 tcttgcagtt ttggagcttg taagtcagag ccccggcctt attgcgacag tccccgccgt
      361 cttggcggcg gtttacggtc ggcgtggatt ggtaaaggtt gaggccagtt cagccgttga
      421 atcatccagc aagtggctcc aggtataggc actggtgagc tggtatccgt ggctaaagcg
      481 cttggttaat gacgctgaaa acgcgtggta cgttgaattg ccgtttggtg taaacaccac
      541 gatgttggcg ccgttgaacc cggcatcccg gaatctggga acatagcttt cagttcctcc
      601 gagcgcgttc aaatcggcca gactcagtga gagaccactc aattgatctg ccgacggcat
      661 ctgcaaaaat gtcgggaggt atcggttttc attgaccacc ggctggcggt taatgcggtt
      721 ctgggtaaac aagttaacgc ctcgggttcc cagatagcga agctccaatc cccaatcatc
      781 tttgagttgc cgctgaatgc tcaggctgta ggaaatcgcc cgtggcacct tctgatctgg
      841 aatccaggcc gatgtaatgc tccgcgccag agttggatcc gtaatcgttg gtccggctga
      901 tggcaaacca ccgcccgcca gatagtttgg aatcgcggct gacggctggg tgttgaccgt
      961 cgcactttcc tgcggcgtgg ttgaaagtgt atacaggttg tcgaaaatca cgtcgtatcc
     1021 aagcgagaaa cccgcccgaa tcgagctttg tccggcattc ccaaaaatct tgttgagcag
     1081 gccatcttta aattcaggtg aataggccag cccgattttc ggagcaaaat tctttttctc
     1141 agccgtaggt tcgttaaatt caagcacgcc aggtacactt gaaatggcat tgagcacctg
     1201 ctgacgtgcc ccaaatggaa cttgctggta ctcgtagcgc acacccagat tcacggtcag
     1261 attttgacga agtcgccagt catcctgggc ataaacatac gccagccact ggttgccgtg
     1321 atagggccgg ccaccgacgt tgcgttccgc cagaaagtcc gggctgatat cgcgcaaaaa
     1381 caggtccgtg tttgaatatt gataatcacc ccggacgcgc tgcacgaaaa agctggtgcc
     1441 aatcaatcgt cggacatcgc cacccagctt gagggaatgg ttgcccttca caatcgagaa
     1501 gttgttaatg acctgataat tgacgtcctt cccaatactt ggagcgcttg aattcggccc
     1561 gatatccaga cctaaatcga gcaatccgat gtttggaaac acatccagcc ctggaaattt
     1621 gaggtttgga accaaaatgt ttggcccatc agaccgccgg aacgagaacc ggaattcgtt
     1681 gttgatcgtc ccggaaaaac tatgcgtgtt ggcataggaa aacagccggc catcggtcgg
     1741 tacaagcgag aaaaacaccg gcagattggc cgaggtgtca ataattcgtt cccgattata
     1801 gatatagcgc acccgatgct gggtcgcgtc gctctgcaga tagtcgaggt taatcaccag
     1861 gttccggcga ttaaagaagg ccggcgaatc aatcgcgaca ttcccaatcg gaatctgacg
     1921 tccaccgact gtaattgagc ctgaatcatt ggctggcgca accgggacat actggttaaa
     1981 cacgctcagg tttgtttgtg ataaccctga caggctgttc aaaatcgcaa acccttcggc
     2041 ggttg
//
LOCUS       DAOQDO010001222         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53724_length_2044_cov_4.615385, whole genome shotgun sequence.
ACCESSION   DAOQDO010001222 DAOQDO010000000
VERSION     DAOQDO010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53724_length_2044_cov_4.615385"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1949)
                     /locus_tag="PKZ53_17490"
     CDS             complement(93..1949)
                     /locus_tag="PKZ53_17490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNJ42285.1"
                     /translation="MPRRNTHPPPSLTDNLPSILESYRFFLRYTRPYRFLFLLAFFGL
                     AVTSGLSLAFPYLFGRLVDVVFRHQNIDRLNSFLYVLVIILVVQIIFNVGRSYCLHYI
                     SEKIVTDIRKDLYAHVLRLSINFFANRRVGEIASRLNADVTSIQATITSNLVDAVRQI
                     AWGIGGVTAMALLNLKMTLLTLTVLPFLVIAAMLVGRVVRSRSLRVQDHLAKANAVLK
                     ESFSGIRTVQAFNREPFEFTRYTAYIEGAHHEALSRAKARSIFTAFAEITLFGGILLM
                     LWFSVGEIKSNRLSAGQLMSFLLYATGVNAAIGAGAGLYLAAQQALGASQRVIELFKT
                     QPEVIDDPKAVPLTKVEGKVEFHNVSFSYPGHEEKRVLDGVSITANPGEVIALVGLTG
                     AGKSTIISLIPRFYDVIHGKITLDGQDIRHIPLVDLREQIAIVPQDAVLFHSTVMENI
                     IYGRVDATEAEVEAAARAAHAHEFIQALPDGYQTVIGERGIKLSGGQRQRLSIARALL
                     KDPKILILDEATSSLDSESERLVQDALDQLMKNRTSFVIAHRLGTIRQATRILVLDEG
                     KIVEEGTHEELLAVDGLYKKLYSLQFRDIQSEDPELAVSEFEFDEEPVETEE"
BASE COUNT          551 a          523 c          496 g          474 t
ORIGIN      
        1 tggttcttgg ttcttggttc ttggttcttg gttcttcgaa agtattggtt ccaaaacacc
       61 aagtactaac cactaaccac tagataagtt cttcattctt cagtttcaac tggttcttcg
      121 tcaaattcaa actctgacac ggctaattct ggatcctcac tctggatatc gcgaaattgg
      181 agcgaataga gtttcttata gagtccgtcc acagccagaa gttcttcgtg ggtcccctcc
      241 tcgacaattt ttccttcatc gagtaccaga atgcgggtcg cctgccgaat cgtgccgagc
      301 cgatgagcaa tcacaaaact ggtgcggttc ttcattaatt gatcaagtgc gtcctgaacc
      361 agccgttctg actcggaatc aagtgaactg gtcgcctcat ctaaaatcag aattttagga
      421 tctttgagca acgcccgggc aatcgaaagc cgctggcgtt gaccgcctga gagtttaatc
      481 ccccgctctc cgatcacggt ttgataccca tcgggcagtg cctgaataaa ctcgtgggca
      541 tgcgccgcac gtgctgccgc ctctacttca gcctcagtcg catccacacg cccatagatg
      601 atgttttcca tgaccgtgct atgaaataac accgcatcct ggggaacaat cgcgatttgt
      661 tcccgcagat caacgagcgg gatgtggcgg atatcctgac catccagcgt gatttttcca
      721 tggatcacat cataaaaccg cgggatcaag gaaataatgg ttgattttcc agctccggta
      781 agcccaacca gtgcaattac ttcacctgga ttggctgtaa tcgagacccc atccagcact
      841 cgtttttctt cgtgcccagg gtaggaaaat gagacattgt gaaattcaac tttgccctca
      901 actttggtga gcgggaccgc cttggggtcg tcaatcacct cgggctgggt tttaaacagt
      961 tcaattaccc gctgtgaggc acctaacgcc tgttgggcag ccagatacag ccccgcgcca
     1021 gcaccgatgg cagcattgac gccagtcgca tacaacaaaa acgacataag ctggccggca
     1081 ctgaggcgat tgcttttgat ctcaccaaca ctaaaccaca acatcaacag aataccgcca
     1141 aacaaagtaa tttcagcaaa ggcggtaaaa atgcttcggg ctttggcgcg gctgagggct
     1201 tcatggtggg cgccttcgat ataagcggta tagcgcgtaa attcaaacgg ttcgcgattg
     1261 aacgcctgta ccgttcgaat cccagaaaat gattctttga gcacggcatt cgcttttgcc
     1321 aggtgatctt gaactcgcag gctccggctg cgaactacgc gaccaaccag catcgcagca
     1381 atcaccagaa atggcaaaac agtcagagtt aacagcgtca ttttcaaatt gagcaacgcc
     1441 atggcggtta cgccgccaat cccccaggca atttgccgaa ccgcatccac cagatttgaa
     1501 gtaatcgtgg cctgaattga tgtcacatcg gcatttaacc gggaggcaat ttccccaact
     1561 cgccggtttg caaagaaatt gatcgacaac ctcaaaacgt gggcatagag gtctttgcga
     1621 atgtcagtga cgattttttc tgagatgtag tgcaagcaat aggatcgccc aacattaaag
     1681 ataatctgca ccaccagaat gatcaccagc acatagagaa acgagttcag acgatcaata
     1741 ttttgatgcc gaaagaccac gtccaccagt cttccaaata aatagggaaa ggccagactg
     1801 agcccgcttg tcacggccag gccaaaaaat gccagcaaaa acaggaaccg gtagggtctg
     1861 gtatagcgga gaaaaaagcg ataactctca agaatactgg gcaggttgtc agtgagggac
     1921 ggaggtgggt gggtgttacg tcgtggcatg tgagaaatcc cagttgagca aaggaacgaa
     1981 ggagcacggg agcaggtagc gagtagcgag taacgaatag cgaaggagat cagtcttacg
     2041 tctc
//
LOCUS       DAOQDO010001223         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53782_length_2043_cov_3.360664, whole genome shotgun sequence.
ACCESSION   DAOQDO010001223 DAOQDO010000000
VERSION     DAOQDO010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53782_length_2043_cov_3.360664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2043
                     /locus_tag="PKZ53_17495"
     CDS             <1..>2043
                     /locus_tag="PKZ53_17495"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ42286.1"
                     /translation="TSIDMGDIDQDGDLDIVVSIDGDLDQILVLTNLGGGFGPESFVI
                     RAYPVGGDPTCIKLADLTGPKDLATGETLPRDGAPEIVMLNRRTGTVATLINSGYGTF
                     GQGRVSESGGINPVSQALGDFNDDGEIDTAVLNRGLDTTQREDSRVCILLGNGDGSFQ
                     PTNPLLLAPSRAVSIAAAGYGVVHRGVEIDIPDINRDGITDIAVVAEQGGGASDIIAP
                     DGSNELASLTVFFGSSLQNGQFTLRGQAQPLVNTVSGGGRIAVYPGADDKLTGLRIGY
                     GALTLFSDFNQNGQIDAYVAGLERNNGRLVSSTVGQVANDFAAGLMTADQLAVQNPIL
                     RYFNVFPSTGDSFASIAAQAPDNVDSISGVVAATSFVVSRTGEIVTQAQNDFSATTGN
                     NAPDLVQVTTNGNVYQSINVAPVVNHAPLMTTANGLADFNGRGRKVIVAEGDEYSIPL
                     KLIDIDNPSANALRYTLLGAPRFVTLTDNFDGTASILIKPGLLDGSNTANNGLGQEYK
                     IVAQVTDNNFPDNDNNSKSPLTSQVVFRVFVPDSMDGLAITTIPDQTAYATQKLTVPI
                     LARDPENRRMTLSTSSTLPFVRIIDQGGGTGLFIIEPTENDGGVYDIDVIVRNDLGLT
                     TTTSFALNVVVNTAPELEPVDNIDMLVAEPQEFEVTATDPDEGQSLRFVLTNAPSF"
BASE COUNT          479 a          532 c          536 g          496 t
ORIGIN      
        1 aacctcaatt gatatgggtg atattgacca ggatggtgat ctggatatcg tggtgtcaat
       61 tgatggtgac cttgaccaga ttctggttct gaccaatctg ggtggaggct ttggccccga
      121 atcttttgtc attcgggcct atccggtcgg tggagatccg acctgcatca aactggctga
      181 tttgactggc ccgaaagacc tggcaacagg cgaaacactc cctcgtgatg gtgctcctga
      241 aattgtgatg ctcaatcgcc gaaccggtac cgtggcaaca ctgatcaaca gtggctatgg
      301 aacctttggg caaggccggg tttcggaaag cggcggaatc aacccggttt cccaggcgct
      361 gggcgatttt aatgatgacg gagaaattga cacggcggtg ttgaaccgtg gattggatac
      421 cacacaacgc gaagattccc gggtttgcat cctgcttggc aatggagatg gctcatttca
      481 gccgaccaat ccattgttgc ttgcaccatc acgtgccgtt tcaatcgctg ccgccgggta
      541 tggagttgtg catcgtggag ttgaaattga tattccagat atcaaccggg acggcattac
      601 cgacattgcg gtggtggctg aacagggcgg tggcgcctct gacatcattg cgccggatgg
      661 aagcaatgaa cttgcctcgc tgaccgtctt ttttggcagc agtctccaaa atggacagtt
      721 tacgcttcga ggccaggccc aaccgctcgt caataccgtc tcaggcggcg gacgaatcgc
      781 cgtctatcct ggtgctgacg ataaattgac tggtctgcgg atcggctatg gggcactcac
      841 cctctttagt gatttcaacc aaaatggcca gattgatgcc tatgtggccg ggttggaacg
      901 caacaatggc cgacttgtca gctcaacagt tggccaggtg gccaatgatt ttgctgccgg
      961 gttgatgacg gctgatcagc ttgcagtcca aaacccaatt ctcagatatt tcaatgtgtt
     1021 tccatcaacc ggcgatagct ttgcctcgat tgcagcccag gcaccggaca atgttgactc
     1081 gatctcaggt gtggtcgcgg ccaccagttt tgtggtgtcc cgaaccggcg aaatcgtgac
     1141 ccaggcccaa aatgacttct ccgccacaac ggggaacaat gccccagatc tggtccaggt
     1201 cacaaccaac gggaacgtct atcaaagcat taatgtcgct ccggtggtca accatgctcc
     1261 attgatgacc acagccaatg gtctggctga ttttaatggt cgtggtcgca aggtgattgt
     1321 cgccgaagga gatgaatact cgatcccact caaactgatt gatattgata atcccagtgc
     1381 caatgcgttg cgctacacat tgcttggcgc cccccggttt gtcacgttga ccgataactt
     1441 tgatggcacg gcttcgatcc tcatcaaacc aggattgttg gatgggtcaa atacagccaa
     1501 taacggactc ggccaggaat ataaaattgt cgctcaggta acagataaca actttccgga
     1561 taatgacaac aattcaaaga gcccattgac ttcccaggtt gttttccggg tttttgttcc
     1621 agacagcatg gatggattgg ctatcacgac cattccagac cagaccgcct atgccacgca
     1681 gaaactgacg gtgccaattc tggcccgtga tccagaaaac cggcgcatga ccttgagcac
     1741 ctcctcaacc ttgccgtttg tgcgcatcat tgaccagggt ggtggaaccg gtctctttat
     1801 catcgaacca actgaaaatg atggtggggt gtatgacatt gatgtcattg tccgcaatga
     1861 tttaggactg accaccacca ccagttttgc cctcaatgtg gtcgtcaaca cggcgccaga
     1921 actcgaacca gttgacaata ttgacatgct ggtggctgaa ccccaggaat tcgaggtcac
     1981 cgccacagat ccggatgaag gccagagcct gcggttcgtt ctgaccaatg ccccatcgtt
     2041 tat
//
LOCUS       DAOQDO010001224         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53834_length_2042_cov_3.077504, whole genome shotgun sequence.
ACCESSION   DAOQDO010001224 DAOQDO010000000
VERSION     DAOQDO010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53834_length_2042_cov_3.077504"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PKZ53_17500"
     CDS             <1..1166
                     /locus_tag="PKZ53_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ42287.1"
                     /translation="VRNTIVVAQVALALVLLLGAGLFLRSFLSLQQTQLGFNPTGILT
                     ARVTIPTARYKDYVSRETFFRAVMREVETFPSLQSAAAVSELPLTGARTATTFQFLNL
                     AGSDPTQSQQTDYRVVTPNYFKTMEIQFLKGRDLTDLDSAKAPGAVVVNETFVSQFLH
                     GEEPIGKKLTFDDPQKSFEIVGVVRTIKHDGALEDTLPEVYMSLQQQPVTSMFLVLRS
                     SNPSSLSSLLQPAVSRIDKDVPVHNVRTLASRLGEYLAPFELNALLTGIFGSVALVLA
                     AIGIFGVMNYVVSQRIHEIGIRMALGAQRPDVVWMILLQGLKLAGFGMAIGLALAMAS
                     LRLLDTLLYGVSPTDSTTFIVVAVTFLGIAALACFIPARWASKVDPLVALRYE"
     gene            1384..1944
                     /gene="hslV"
                     /locus_tag="PKZ53_17505"
     CDS             1384..1944
                     /gene="hslV"
                     /locus_tag="PKZ53_17505"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNJ42288.1"
                     /translation="MVRGSISSRTPEIRSTTVLAVRRDNKVVMVGDGQVTMGPTVVKS
                     GARKIRRLYNEKILAGFAGSTADAFALFGRFEAKLEQYHGQLFRSAVELAKDWRTDKF
                     LRHLEALLLVADEKSILMISGTGDLIEPDEPVMAIGSGGPYAIAAARALLAHTQLPAR
                     TIAEEAMKIAADICIYTNPNLIIEEL"
BASE COUNT          438 a          524 c          536 g          544 t
ORIGIN      
        1 atgtgcgcaa caccattgtg gtggctcaag tggcgctggc gctcgtgctc ttgctcggcg
       61 ccgggttgtt tctccgaagt tttctctcgc tccagcaaac acagcttgga tttaacccaa
      121 ccggcattct tacggccagg gtgacaattc ccacggctcg ctacaaagat tatgtctcgc
      181 gcgagacatt tttccgggcc gtgatgcgtg aagtcgaaac ctttccgagc ctccagtcag
      241 ccgcggcggt ttctgaattg cccttaaccg gtgcccgaac cgccaccacg tttcaatttt
      301 tgaatctggc ggggagcgac cccacccaat cgcagcagac tgactatcga gtcgtaactc
      361 cgaattattt caaaacaatg gagatccagt ttctgaaagg acgggatttg actgatctgg
      421 atagtgccaa agccccagga gctgtggttg tcaacgaaac gtttgtgtcc cagtttttac
      481 acggtgaaga gccgattggg aaaaaactga cgtttgacga tccccaaaaa tcttttgaaa
      541 ttgttggtgt cgtgcgcacg atcaagcacg atggagccct tgaagacaca cttcccgaag
      601 tctatatgag cctccagcag cagccggtga cctccatgtt tcttgtcttg cgcagctcca
      661 atccttccag cctcagttcc ttgttacaac cagccgtcag ccggattgat aaagatgtgc
      721 cggtgcacaa tgtgcgtacc ctggcttcgc gattggggga atatctcgcg ccatttgaac
      781 tcaatgctct gttgaccggt atctttggca gtgtggcgct ggtgctggca gcgattggga
      841 tttttggagt gatgaattat gtcgtttccc agcgaatcca tgaaattggc atccggatgg
      901 cacttggtgc ccagcggcca gatgtcgtgt ggatgatcct ccttcaagga ctcaaactgg
      961 ctggatttgg catggccatt ggtttggcgc tggcaatggc atcgctccga ttgctggata
     1021 cgttgttgta tggagtaagc ccaaccgatt caaccacctt tattgtggtt gctgtcacgt
     1081 ttcttggaat tgccgcgctg gcctgtttca tcccggcacg ctgggcttca aaggttgatc
     1141 cgctggtggc actccgatac gaatagggtg acaactcatt tccgccacct gtcactgtgt
     1201 cattttgtca cgttgtcacc ttgtcattct gtcatcttgt tatcttctca gatggtcact
     1261 ttgtcactcg ttgcccaacc tgatttgcgc cagtaggggg attgtgataa acctttggga
     1321 cttgtctctt gagcaagtat tttgcccctg cagcaggaaa ggttcctcta tctaatgact
     1381 tcgatggttc gcggatcaat ttcttctcga acaccggaaa tccggagtac aaccgttttg
     1441 gcggttcgac gtgataacaa agtggtgatg gttggtgatg gccaggtcac gatgggccca
     1501 actgtggtca aatctggcgc ccgcaagatt cgccggctgt acaatgaaaa aatcctcgcc
     1561 gggtttgctg gctcaaccgc cgatgccttt gcgttatttg gccgcttcga agccaagctt
     1621 gagcaatatc acgggcagct tttcaggtca gcggtcgaac tggccaaaga ctggcgaacc
     1681 gataaatttc tccgccatct ggaagccctg ttgctggtgg ctgatgaaaa gtcaatcttg
     1741 atgatttccg gcactggcga cttgattgaa cctgacgaac cagttatggc gattgggtct
     1801 ggcggcccgt atgcgattgc cgccgcgcgg gcgcttctgg ctcatactca gcttccagca
     1861 cgcacgattg ccgaagaggc gatgaaaatt gcggcggata tttgcattta taccaatcca
     1921 aacctgatta tcgaggaact gtaagcgcga ctctcccggc acaatcagtg cgccgccagt
     1981 tgatcggtca agcggttgaa ggtgaaaaat tattgaattc aaatggctta taagctctaa
     2041 tc
//
LOCUS       DAOQDO010001225         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53843_length_2042_cov_2.497735, whole genome shotgun sequence.
ACCESSION   DAOQDO010001225 DAOQDO010000000
VERSION     DAOQDO010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53843_length_2042_cov_2.497735"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2042)
                     /locus_tag="PKZ53_17510"
     CDS             complement(<1..>2042)
                     /locus_tag="PKZ53_17510"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ42289.1"
                     /translation="CGSSHALIGYVYWMIDEYAISRDHLLAGLDHLEAVGDARALAQT
                     YWNLSLVDYLEGRQQESREDSMRGLELLEKMSSRPTVADHISIGKLLNNLAMVEIEEG
                     NIHKAVANYERAIEHWSQTKDRNLLGIAYNNLADAQLGIGEWQRAETSLKMAFDLATG
                     INKRTESVVLDTLGELYLRQGRFQDAERCVRRAIELALETGIKSIEAMGWETLGDVFL
                     ARNRVDDALVQFQHTLQLNSKIGRLVDIPGTYLRLATGYLQRSDIDKAADYLKQAQEL
                     LKTQPNLHMSGLAARYEGIIRLSRGESTEAISLLAQSISIFESISFAYEAACSHLEIG
                     LAMARTANPQRAMSHLEKACKIFTDLKAEPRQKQATEAIERLLDNSYSMRPESAALTM
                     DTLIVERLITAATSHDLLLREVATIIRDELQMQAVVFEQLADESLKLVTARGCDRKEA
                     DRAFFELRLHLEEEKPLPPGVQLKLLADNRHTNDPTPLRRFWLYLSGGTPASATKLGN
                     LDSLLRVVELGLENCMLRNTVRASQTIVSSYKTSSNLVAAGFVCESPAMMRALERIQK
                     IRSSDVTVLITGESGVGKELIARAVHNTSARSARPFLPFNCAAIPAELVESRLFGHRR
                     GAFTGADKDSFGIIRAASGGTLFLDEIGELTLHVQPKLLRFLQDREIHPVGEDHPV"
BASE COUNT          449 a          531 c          565 g          497 t
ORIGIN      
        1 gaccggatgg tcttcaccga ccgggtggat ttcgcgatct tgtaaaaagc gtagtaactt
       61 gggttgaacg tggagggtga gttcaccaat ttcatccaaa aacagcgttc caccgctggc
      121 tgcccggatg ataccaaagg aatccttgtc ggcaccggta aacgcaccgc gccgatggcc
      181 aaacaaccgg ctttccacca gttcagccgg gatcgcggca cagttaaacg gcaggaatgg
      241 cctggcgctc cgggcgctgg tgttatgaac tgcccgggca atgagttctt tgccaacgcc
      301 agactcaccc gtaatcaaga ctgtcacgtc agatgagcgg attttttgaa ttcgctccag
      361 tgcgcgcatc atggccgggg attcacagac aaatccagcc gcaaccaggt tactgctggt
      421 tttatagctg ctgacaatgg tttgcgaggc ccgaactgta ttgcgcagca tacagttttc
      481 aagacccaat tcgacgaccc gcaacagtga atccaggttt ccaagtttgg tggccgaagc
      541 cggtgttccc ccactgagat agagccagaa gcgacgaagt ggagttgggt cattggtgtg
      601 tcggttgtca gccagtaatt tcagttgaac gcctggtgga agtggttttt cttcttctaa
      661 gtgcagccgg agttcgaaaa acgcgcggtc agcttctttg cggtcacatc cacgagccgt
      721 gaccagtttc agactttcat cggcgagttg ttcaaacacc accgcctgca tttgcagttc
      781 atcccggatg atggtggcaa cttcgcgcag gagcaaatcg tgtgacgtgg ctgccgtaat
      841 cagccgctcg acaatcaggg tgtccatggt gagcgcggct gattccgggc gcatggaata
      901 tgagttgtcg agcagtcgtt caatcgcttc agtcgcctgt ttttgcctgg gctctgcctt
      961 gaggtcggta aaaatcttgc aggctttttc cagatgagac atggcgcgtt gtggattggc
     1021 cgtccgggcc atggccagac caatttccag atggctgcac gcggcttcat aggcaaagct
     1081 gattgactca aaaatggaaa tactttgtgc gagcagggaa attgcctcgg ttgactcgcc
     1141 tctggagagt cggatgatgc cttcatagcg agccgccagc cccgacatgt gaaggttcgg
     1201 ctgggttttg agcaattcct gagcctgttt gaggtaatca gccgctttat caatatcaga
     1261 gcgctgcaag tagccagtgg cgagtcgcaa gtaggttcca ggaatatcca ccaggcgacc
     1321 aattttggag ttaagttgaa gcgtatgctg aaactgaacc agggcgtcgt caacccggtt
     1381 tcgggccaga aagacatctc caagtgtttc ccaccccatg gcttcaattg atttgatccc
     1441 ggtctcaagc gccagttcga ttgcccgtcg aacacagcgt tctgcgtctt gaaatcgtcc
     1501 ctggcgcaga tacagttcgc cgagggtatc gagcaccacg ctttcggttc gtttgttgat
     1561 gccggtggcc agatcaaacg ccattttgag cgacgtttca gcccgttgcc attcgccaat
     1621 ccctagttgc gcatcagcca gattgttgta agcaattccg agcaggttgc ggtctttggt
     1681 ttgtgaccag tgttcgatgg cgcgttcata attggcaacg gctttgtgaa tattgccttc
     1741 ctcaatttca accatcgcca ggttattgag gagtttgcca atagagatgt gatcggccac
     1801 cgtcgggcga ctggacattt tttcgagcaa ttccagcccg cgcatcgagt cttcgcgtga
     1861 ttcctgctga cggccttcaa ggtaatcaac cagggaaagg ttccagtagg tttgggccag
     1921 tgcccgggca tcgccaacgg cttccaggtg atcaagcccg gccagcaggt gatcacgtga
     1981 aatcgcatat tcatcaatca tccagtacac atagccgatc aaggcatggg atgatccgca
     2041 cc
//
LOCUS       DAOQDO010001226         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53886_length_2041_cov_3.301108, whole genome shotgun sequence.
ACCESSION   DAOQDO010001226 DAOQDO010000000
VERSION     DAOQDO010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53886_length_2041_cov_3.301108"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1255)
                     /gene="bshA"
                     /locus_tag="PKZ53_17515"
     CDS             complement(113..1255)
                     /gene="bshA"
                     /locus_tag="PKZ53_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNJ42290.1"
                     /translation="MNIGITCYATYGGSGIVGSELGMELARRGHQVHFISSALPTRIT
                     ELNDNVHFHEVEIANYPLFDHPPYTLALAVKMAEVIREQKLDLLHVHYAIPHSVAAFL
                     AREMLVDFKHIPFVTTLHGTDITLVGADRSYLPITRFGILKSDGVTAVSQYLKTATCE
                     VFDVPQDVIEVIPNFISPQIYQRKPQDCLLEKCAPKGEKILMHISNFRAVKRVTDCIR
                     IFARLNGKVKAKLLMVGDGPEKSQAEWLARQLGVHKDVVFAGKQPNIPAYLSLADVLL
                     LPSETESFGLSALEAMACEVPVVASHVGGLPEVVIDGETGYLADVGDIEAMAKYVQKI
                     LTSESLGKKMGALGRQVAIERYSAASIIPHYEAYYQKVLDRAKAQG"
     gene            complement(1386..>2041)
                     /locus_tag="PKZ53_17520"
     CDS             complement(1386..>2041)
                     /locus_tag="PKZ53_17520"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNJ42291.1"
                     /translation="IQGGQQLKAPPTSCTVPSDFSSAAFFLAAALMVEEADLTIRHVG
                     MNPTRTGMLQLLQSLGAEIQIENERFEIGEPVADLRVTRHHRVVAAPLVLKGDIIPNV
                     IDELPILAVLGACLGGVEIRDARELRVKESDRIRLVVENLRRLGAEVTEFEDGLSAPG
                     NQPFRGAVIDTAGDHRIAMAFAIAALVADGNTHIQGAECVEVSFPKFYNVLHAIRQA"
BASE COUNT          492 a          572 c          493 g          484 t
ORIGIN      
        1 tcagcccact gtcttcaacc cccagccctt agcccccagc ccgctgtctt cagcccactg
       61 tcttcaaccc tcagccctta gccccaagcc cgatgtcttc agcccgaaga cttcagccct
      121 gtgctttcgc tcgatccagc actttctgat aataggcttc atagtgcgga ataatactcg
      181 ctgcgctgta tcgttcgata gctacttgtc gcccaagcgc gcccattttt ttgcctaatg
      241 actcgctcgt caatattttt tgaacatatt ttgccatggc ttcaatatcg ccgacatcgg
      301 ccaggtatcc ggtttcgcca tcaatgacga cttcaggcag gccgccgacg tgactggcaa
      361 cgactggaac ttcgcaggcc atggcttcaa gcgcggaaag tccgaaggat tcagtttcgc
      421 ttggcaacaa caagacatca gccagcgaca ggtaagccgg gatattgggc tgtttaccgg
      481 caaagaccac gtctttgtga accccaagct gacgcgccag ccactcagcc tgtgatttct
      541 cagggccatc gcccaccatc aacaatttgg ctttgacctt gccgttgagc cgggcaaaga
      601 tgcggatgca atcagttacc cgcttgacgg cgcggaaatt tgaaatgtgc atcaggatct
      661 tttcgccttt gggagcacac ttctcaagca aacaatcctg cggtttgcgc tgataaattt
      721 ggggtgaaat aaagttaggg atcacttcga tcacatcctg gggcacgtca aatacctcac
      781 aggtcgcagt tttcaggtac tgggaaactg ccgttacccc atcacttttc agaatcccaa
      841 accgcgtaat cggaagatat gaacgatcag ccccaaccag tgtaatatca gtgccatgca
      901 gggtagttac aaacggaatg tgtttgaaat caaccagcat ttcacgcgcc aggaaggcgg
      961 ctactgagtg aggaatggca taatgaacat gcagcaaatc cagtttttgt tcgcggataa
     1021 cctcagccat tttgaccgcc agcgccaggg tatagggagg atgatcaaag agcggataat
     1081 tggcaatttc gacttcgtga aaatgcacgt tgtcgttgag ttcagtgatt cgggtgggca
     1141 aagccgaaga aataaaatgc acctgatggc cacgccgggc cagttccatg cctaattccg
     1201 agccgacaat accgctcccg ccatatgttg cataacaggt aattccaatg ttcaccgtac
     1261 acccccaact ggttggtttc tctattttca gggcctgatt gattcatcgt ggtttctggg
     1321 atgtggtatt tcgggttctt tgacagtacc catcaccaga gatttgaaaa tgacccgagt
     1381 gtatttcacg cctgcctgat agcgtgtaaa acgttgtaaa acttcgggaa agaaacttca
     1441 acgcattcag ccccttgtat atgggtgttt ccatcggcaa ccagggcggc aatggcaaac
     1501 gccatggcaa tgcggtggtc tccggcggta tcaatgacag cacctcgaaa gggttgattg
     1561 cccggcgcgc ttaatccgtc ctcaaactcg gtgacttcag cgcccagccg ccgaaggttt
     1621 tcaaccacca gccggatacg gtcgctttct ttgacgcgga gttctctggc atcgcgaatt
     1681 tcaaccccac ctaaacaggc cccgagcaca gccagaattg gcaattcgtc tatcacattg
     1741 ggaatgatgt ctcccttgag caccagcggt gccgcgacca cccgatgatg acgggtgacc
     1801 cgcaaatcag ccactggttc gccaatctca aagcgttcgt tttcaatctg aatttcagcc
     1861 ccaagcgact gcagcaactg aagcattccc gtccgggtgg ggttcatccc gacatggcga
     1921 atggtcaaat cggcttcttc aaccatcaac gctgccgcta aaaagaaggc cgcagacgaa
     1981 aagtctgatg gcaccgtgca actggtcggc ggtgctttca actgctgacc gccctggatt
     2041 c
//
LOCUS       DAOQDO010001227         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53892_length_2041_cov_2.988419, whole genome shotgun sequence.
ACCESSION   DAOQDO010001227 DAOQDO010000000
VERSION     DAOQDO010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53892_length_2041_cov_2.988419"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKZ53_17525"
     CDS             complement(<1..715)
                     /locus_tag="PKZ53_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNJ42292.1"
                     /translation="MDAQAILTAFQDHLEYTLAKDRFSTTDLDRYMALSYTIRDRLIE
                     RWLRTQDTYYQHDSKRVYYLSMEFLIGRTLTNSLINLGLYDECRKALEEMDLELESVA
                     EVEWDAGLGNGGLGRLAACFLDSMATLGLPGYGYGIRYEYGIFNQRIKNGNQVERPDN
                     WLRFGNPWEIPRPQVLYPVNFYGHVLQYVDRKGHLVSEWLETTEVMAMAYDIPVPGYQ
                     TNTVNTLRLWSAKASRDFDF"
     gene            complement(1055..>2041)
                     /gene="aroE"
                     /locus_tag="PKZ53_17530"
     CDS             complement(1055..>2041)
                     /gene="aroE"
                     /locus_tag="PKZ53_17530"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNJ42293.1"
                     /translation="GMGAPGAPTRILGPALGSYVTYSAAPDGNLSAPGQFSAADLRHV
                     YRTHRLSTATKVTGLIGKPVGHSLSPHMHNAAFEALGLDWIYLPFEVQPEGTDLADFI
                     HRVVHPKTRQVPFQSTGFSVTIPHKQAVIPLLDSLTTTAEAVGAVNTLLIEGTRLIGH
                     NTDVDGAMRPLEKRFALAGSRVAVLGAGGSAHALVYGLVQRQVQVTLFARNQEKARSL
                     ADKFGIRCCSLEDFCGQQFEGAVNCTPLGMHGTANPAASPLAPEQLGGLHWVYDLIYR
                     PRLTPLLAAAQAQGIATLDGLEMLVEQARLQFATWTGQEISFELMFAAAQKG"
BASE COUNT          488 a          518 c          557 g          478 t
ORIGIN      
        1 tgaagtcaaa atctcgtgag gctttggctg accagagtcg caatgtattg accgtattgg
       61 tctgatagcc gggaactgga atgtcatagg ccatggccat gacttcggtc gtttcaagcc
      121 actcgctcac gagatggcct ttgcggtcaa catattgcag cacatgacca taaaaattga
      181 ccggataaag cacctgtggc cgtggaattt cccatgggtt accaaatcga agccagttgt
      241 caggtcgctc aacctggttc ccatttttga tgcgctggtt gaaaatgccg tattcatatc
      301 gaatcccgta cccatagccg ggcaatccga gggtagccat tgaatcgagg aaacaggctg
      361 ccagtcggcc cagaccacca tttcccagac ccgcatccca ttcgacttca gccactgatt
      421 caagctcaag atccatttct tccagggctt tccgacattc atcatagagg cccaggttaa
      481 tcagactatt ggtaagcgtg cgcccaatca gaaactccat cgaaaggtaa tagacgcgct
      541 tggaatcatg ctggtaataa gtgtcctggg tgcgcagcca gcgttcaatc agacgatccc
      601 gaatggtata gctcagtgcc atatagcggt cgagatcggt ggttgaaaag cggtctttgg
      661 caagggtgta ttcaagatgg tcctgaaacg cggttaagat tgcttgagca tccatgccgt
      721 gatagagggc atgcacattg agttcttgct ttgagttggc catgaggtca cctttgattg
      781 gggggaacaa atgttaatct gcgccatgca cattgcaggg aggtcaccgt caggaggaat
      841 cgtgggcaat gcttgggacg tcggctgcaa cgacacaggg cagatggcga attgagattg
      901 gggcaacgcc gggtgaatta tgaattatga attatgaatt atgaaatagg cagagtggtg
      961 aagtaggtga ctttgggctt tctctagcag gttcttttga ggttatccta ttgatttgtc
     1021 aatagttaaa agttcatgac tcataactca taattcatcc tttctgggcg gcggcaaaca
     1081 tcagttcaaa tgaaatctcc tggccagtcc aggtagcaaa ctggaggcga gcctgttcaa
     1141 ccagcatttc cagtccgtca agtgtcgcga ttccctgtgc ctgagccgcc gccagaaggg
     1201 gtgtcaggcg cggacggtaa atcaaatcat acacccagtg caatccaccg agttgttccg
     1261 gtgccagggg gctggcagcc gggttggctg taccgtgcat tcccagagga gtgcagttga
     1321 cagcgccttc aaactgctga ccgcaaaaat cttccagcga acaacacctg attccaaatt
     1381 tatcagccag tgaccgggcc ttttcctggt tgcgggcaaa aagcgtgacc tgtacctggc
     1441 gttgaaccag cccatacacc agggcgtggg ctgatcctcc ggccccaagt accgccaccc
     1501 gtgagccagc cagcgcaaaa cgtttttcaa gcggacgcat ggcgccatcc acgtcggtgt
     1561 tgtggccaat caaccgggtt ccttcaatca acaatgtatt gactgctccg actgcttcgg
     1621 cggttgttgt cagactgtca agcaacggga tcaccgcctg tttgtgcggg atcgtgacac
     1681 taaatcctgt tgattgaaac ggaacctgac gggttttcgg gtgaaccaca cggtgtataa
     1741 agtcagccag atcagtgcct tcgggttgca cttcaaacgg aagatagatc caatccagcc
     1801 cgagggcttc aaacgcggca ttgtgcatgt gtggtgaaag cgagtgcccg accggttttc
     1861 caatcaatcc cgtgactttg gtcgccgttg agaggcggtg ggtacggtac acgtgacgta
     1921 agtcagccgc tgaaaactga cctggtgccg atagattccc atctggcgcg gcggaatagg
     1981 tcacgtaact gccaagcgcc ggccccaaaa tccgggttgg cgcgccgggt gctcccatcc
     2041 c
//
LOCUS       DAOQDO010001228         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53909_length_2041_cov_2.348943, whole genome shotgun sequence.
ACCESSION   DAOQDO010001228 DAOQDO010000000
VERSION     DAOQDO010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53909_length_2041_cov_2.348943"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..>2041
                     /locus_tag="PKZ53_17535"
     CDS             385..>2041
                     /locus_tag="PKZ53_17535"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNJ42294.1"
                     /translation="MTHRIMFLGFVLFTMIVGFGTYSRSHSQAALQPAPSTIQPTLTP
                     AYLLQSNPSSTQSRPPSERFQEVQLAFLQSDGKLQERLAPGLERRVSGTLEQMEIEWT
                     VTPGGNPSDIRLEFPAVERFEYTAEKSLILHLGDRHQIEVSDLKAQQTLPNQRVDVPA
                     RIEYLGESQIGITAGAYVTGEPLRISLRVKSDLELIKEYSSNATQPGSHSHTTEADRM
                     VARPASAGKRGQFSGAATDVFTVDPVPDIAIPDNGYNGTLGSMASVTIDTSSLPVGST
                     VDDVTISIDLTHTFIGDLTIKLESPSGSVLGIVSRTGAVETADDGNDNAGFGENSNWG
                     GSILFSDAGPVSAEQMGKVPADLTTIDTVCVTNGQCHYSPAPGSIATPPSTFAGFDGQ
                     NARGNWRLYVGDSAAADTGTLRSVTLTLTTSPSSTTVSSINTSGANPLCNTISGTTVT
                     WDVTFAASVTGVTASNFSLVDVGNTLTGESISSVSGSGTAWTVTANTGTGAGTLRLDM
                     ANSTGITPTVTNVPFTGQTFTINAPATVNAGPDQTVCAGSAVTL"
BASE COUNT          483 a          529 c          517 g          512 t
ORIGIN      
        1 acaactgaca acacggacga aaaaacgaaa acctgaagtg aagcgggcat cttggacaat
       61 tccactgctt gcttcacttc aggttttccg ttttatcctt gtcaatgtcg tctttttgtc
      121 catgttccca caccgagtgc attgagttta gtctggtgtt tcacccgtac actagatatt
      181 tttgtcggtc tcctggattt ttcgatctaa attggataca ttgcgacagt tcactttatc
      241 cacagtaccc atcaatactt gatcccttcg tttcaaatca tccccccagt ttgatttcag
      301 ccgtcaccta ttatttttcg ctactcagaa atcccaattg ctcttgagcg atatggggaa
      361 cccctggttt ttgaaaggtt ttatatgacc caccgaatta tgttcctggg atttgtgctt
      421 tttacgatga ttgttggttt cggaacttac tccaggtctc attctcaggc tgccttacaa
      481 ccagcaccct caacaattca acccactttg acaccagcct atcttctgca atcgaatccg
      541 tcatccaccc aaagccgtcc ccccagtgaa cggtttcagg aggtacaact ggccttttta
      601 caatcagatg gcaagttgca agagcgtctt gcgccagggc ttgagcgccg agtcagcggt
      661 accctggagc aaatggaaat tgagtggact gtgacgccag gaggcaatcc atctgatatt
      721 cggcttgaat tcccagcggt cgaacgcttt gagtatacgg ctgagaaatc actgattttg
      781 cacctgggtg atcgacacca gattgaggtc tctgacctga aggcccagca aacgttgcca
      841 aatcaaaggg ttgatgttcc agccagaatt gaatatctgg gtgagagtca aatcggaatc
      901 acggcggggg cttatgtgac gggggaaccg ctccggattt ccttgcgcgt gaagtcggat
      961 cttgagttga tcaaagagta ttcaagcaat gccacacaac ctggttctca ttctcacacc
     1021 actgaggctg atcggatggt tgcccgaccg gcaagcgctg gcaagcgggg gcaattttca
     1081 ggcgcggcaa ccgatgtctt tacggttgat ccagtaccgg acatcgctat tcctgataat
     1141 ggctataacg gaacattggg gagtatggct tccgtcacga ttgataccag tagcctcccc
     1201 gttggttcaa ccgtggatga tgtcaccata tccattgacc tgacgcatac ttttatcggc
     1261 gatttgacaa tcaaactgga aagtccgagc ggttcggttc ttggaatcgt cagtcgtact
     1321 ggtgcggttg agacggcaga tgacggcaac gataacgccg gttttggcga aaacagcaac
     1381 tggggtggaa gtattttgtt cagtgatgcc ggcccagtca gtgctgagca aatgggcaaa
     1441 gtcccggctg atcttacaac catcgacacg gtttgtgtga ccaatgggca atgccattat
     1501 tctccggcgc cagggtcaat tgcgactcca cccagtactt tcgccgggtt tgacggtcaa
     1561 aatgctcgtg gaaactggcg actctatgtc ggagacagcg cggcggcgga taccggtacc
     1621 ctccgaagcg tcacgttgac actgacgacc tcaccgtcat caacgacggt ttcctcaatc
     1681 aatacatctg gcgcaaaccc tttgtgcaac acaatttccg gaacgaccgt gacctgggat
     1741 gtgacgtttg ccgcatccgt gaccggagta acggccagta acttcagtct ggtggatgtc
     1801 ggaaacacat tgactgggga aagcatttcg tcggtgtcag gttccgggac agcctggacg
     1861 gtgacagcca acacgggcac cggcgccggg acgctccggt tggatatggc gaactctacg
     1921 ggcatcacac caacagtgac gaatgtaccc tttacgggac aaacctttac catcaatgca
     1981 ccagccacag tgaacgccgg gccggatcaa acagtttgtg ctggatcggc ggtgacgctg
     2041 g
//
LOCUS       DAOQDO010001229         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_53997_length_2039_cov_3.428931, whole genome shotgun sequence.
ACCESSION   DAOQDO010001229 DAOQDO010000000
VERSION     DAOQDO010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53997_length_2039_cov_3.428931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PKZ53_17540"
     CDS             complement(<1..1245)
                     /locus_tag="PKZ53_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ42295.1"
                     /translation="MPAEARTQKVPVAVRKRVLLMGTSVEVVSHLQRILRRSRVFQLH
                     VITHSAALVATLADETFDYVLVDLTLGDETIWDDLLYLRQKLAEVPVVGLLNQPHPVL
                     ESRARQEGVLTFLTPPFTAQAIRKTLTIATDRLSDHVAFHRSLAYIEASSQIHVSLNL
                     KEVIEAVIRGLTRLTPCQKIRILLSEIDPEKPALGFEATFSELGRPTVRPLDRDEASI
                     ELATASLQKETLYQENILTVPIRCDDDQVGSMSLRHPRSLRLKDHELQLIENLARHLA
                     IGVRNAQRYDAMHHSHNQIFLINEVGRQVASCLEFDTILTDIVDHVRRYFNYDIVALY
                     LPILAGPHGQNHYLASRIGNDVVIRTSVLDGDLGLIHYVMNTQQTVICHDLHRQTNWK
                     GYHTGSRSKLVSPIWMEGNIAGT"
     gene            complement(1470..>2039)
                     /locus_tag="PKZ53_17545"
     CDS             complement(1470..>2039)
                     /locus_tag="PKZ53_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ42296.1"
                     /translation="SQGNALVCGHSILTAPLHVKARVGYLPESGALFEKLSAREYLTL
                     TGQLHKLPSDQLPGCVQEWLKFFGIADKADQQMATFSKGMKQKVCWIAALMHNPDVLI
                     LDEPLNGLDVETVAKAKELMKYLVACGKTIFYSSHLIDIVEKVCTRIAVLNAGRLIGV
                     GTIPEIVAFSGEPTLEQALLKLWQGHPLL"
BASE COUNT          509 a          513 c          524 g          493 t
ORIGIN      
        1 ggtcccggca atgttacctt ccatccagat tggtgaaacc agctttgagc gcgacccggt
       61 gtggtatcct ttccagttgg tttggcggtg gagatcgtgg caaatcaccg tttgctgggt
      121 attcatcaca taatgaatca atcccaaatc cccatcgagc accgaagtcc ggatgacaac
      181 atcgtttccg atacgactgg ccagatagtg attttgccca tgcgggcctg ccagaatcgg
      241 caggtagagt gcaacgatgt cataattaaa ataccgccgg acatgatcga cgatatcagt
      301 caagatggta tcaaattcaa gacaactggc gacctgacgg ccaacttcat tgatcagaaa
      361 aatctggtta tgactgtggt gcatcgcatc atagcgctgg gcattgcgga ccccaatcgc
      421 cagatggcgg gccagatttt cgatcaattg aagctcatgg tcttttaacc gcaggctgcg
      481 tgggtggcgc agactcatcg aaccaacctg atcgtcatca cacctgatcg gaaccgtcag
      541 aatattttcc tggtacagcg tttctttctg gagtgaggcc gtcgccagtt caatggaggc
      601 ttcatcacgg tccagcggtc gcaccgtggg gcggcccagt tctgaaaatg tcgcttcaaa
      661 gcccagggcg ggtttttccg ggtcaatttc actgagcaaa atacggattt tctggcaagg
      721 ggtgagacga gttaaccccc ggataacagc ttcgatcact tccttgaggt ttaatgaaac
      781 atgaatctgg ctgctggctt cgatataggc caggctccga tggaaggcaa catggtctga
      841 gagccggtca gtggcaatag tcagggtttt tcgaattgcc tgcgcggtaa atggaggtgt
      901 cagaaacgtc aggacccctt cctgacgggc acgtgattca agcactggat gaggctggtt
      961 gagcagtccc acgaccggaa cttcagcgag tttttggcgc aggtacagca aatcatccca
     1021 gatggtttca tctccaaggg tcaaatcaac cagtacataa tcaaaggttt catccgccag
     1081 cgtggccacc agagccgcgc tgtgggtaat cacatgcagt tgaaaaactc gactccggcg
     1141 cagaattcgc tgcaggtgag agacaacttc gacacttgtt cccattaaca acacacgttt
     1201 gcggacagcg acaggcacct tttgagttcg ggcttctgca ggcataaagc aatgaagtat
     1261 gggaagcggc tattggaact gaagaatgat tgtatgaatg gcttgcggtt tgcagaccac
     1321 tctagcgatt ttcgtagacg aagcgcaagc cttcgggttc aatccctgaa agtcgcgctt
     1381 gatttcaaaa accagactca atgataccgt gactctatga ggattaacga cctttttcat
     1441 ctggttctgc tgtcattctc tttgagtggc taaagcagcg ggtgcccctg ccatagtttt
     1501 aagagggcct gttccagggt gggttcacca gagaacgcca caatttcagg aatcgtcccg
     1561 actccaatca accggcctgc attgagaacc gcgatccggg tacacacttt ctcgacaata
     1621 tcgatcaggt gcgacgagta aaaaatggtt tttccacagg ccaccaggta cttcattaat
     1681 tctttggctt tggcgaccgt ttcgacatca agcccgttga gcggctcgtc caaaatcaaa
     1741 acatccgggt tgtgcatcaa agcggcaatc cagcagactt tctgcttcat cccctttgaa
     1801 aatgtcgcca tttgctggtc ggctttgtca gcaattccaa aaaatttaag ccactcctga
     1861 acacaacccg gaagctgatc cgacggtaat ttatggagtt gcccggttaa ggtgaggtat
     1921 tcacgggcag acagcttttc aaataaggcg ccggattcgg ggagataccc cacacgtgct
     1981 ttgacgtgca acggagcggt taatatgctg tgaccacaca ccagagcatt gccctgact
//
LOCUS       DAOQDO010001230         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54027_length_2039_cov_2.432964, whole genome shotgun sequence.
ACCESSION   DAOQDO010001230 DAOQDO010000000
VERSION     DAOQDO010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54027_length_2039_cov_2.432964"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1324
                     /locus_tag="PKZ53_17550"
     CDS             179..1324
                     /locus_tag="PKZ53_17550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42297.1"
                     /translation="MASHDEKLTAYLLGELPEEEQVELEQHYLKDDDLFEQVEIVEDE
                     LIVAYLLHQLPSERKHQFESVFLLPPERQEKYKNLKAILEFANHPDQVPVQVVQIPDS
                     GERVSWWEAVKAFFRPAFSPGGLILAGLTAMFVLATTLSIIAFRSHRSSEGPSTTAET
                     TPPPVLPPVTINPNPELVQQIETLKADRDRERIQREQAEKQLAELTSTVTRLKIKNKK
                     PGGVAPEGDQTRTADSTSGNTPVTTIPSNLTLPALEDDLLWQFISNSQKPEDFLGYLK
                     LYPSGKHADEARAALADLRPQLLSEAHNTLGISHFRLGEYDPALTEFHKALELRPQFP
                     EAQFNLGLTLVKLGRLDEAQKLVSPIKQAKPSLARQLEAELRKARKN"
     gene            complement(1482..>2039)
                     /locus_tag="PKZ53_17555"
     CDS             complement(1482..>2039)
                     /locus_tag="PKZ53_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ42298.1"
                     /translation="VRAAEVRDTLESLVAQGKIRFYAWATEDPDRIRLFAAGDHCTAS
                     PQLLNLVDANQQTLDLCDEFDLACIARRPLGMGLLTGKYDAGSSFAENDMRRRFGWNL
                     KDGKQAQILNKINHLQDILTRGGRTLTQGALAWIWARHPRAIPIPGFKTVAQVEENLK
                     ALDFGPLSTEDFDYIERIKSVVSRQ"
BASE COUNT          533 a          547 c          473 g          486 t
ORIGIN      
        1 atgtttacca ggttctgatc ggaactccca ggcagtcagt gacgcgcctt ttcttgactt
       61 ttgaatgtcc acctatccac cggagaacga aacgcgacat ctctttccct ggtgtcattg
      121 agccaccaaa ccgggttcct ggcgccgggc accctcgata gacgctgaag aatttcttat
      181 ggcatctcac gatgaaaaac taacagctta tctcctcggt gaactccccg aggaagaaca
      241 agttgagctt gagcaacact atctcaaaga tgatgatttg tttgaacagg tggaaatcgt
      301 tgaagatgaa ctgattgtcg cgtatttact ccatcagctc ccttcagagc ggaagcacca
      361 gtttgagtcg gtttttcttt tgccccccga acgccaggaa aaatataaaa atctcaaagc
      421 tatccttgag tttgccaatc atccagacca ggtcccggtt caggtggttc agattccaga
      481 ctcaggagaa agggtaagct ggtgggaagc tgttaaggct ttttttcgac ctgctttttc
      541 acccggaggc ttaatcctgg ctggattgac ggccatgttc gtcctggcca caacgctttc
      601 gatcatagcc tttcgctccc atcggtcatc tgaaggacca tccaccacgg cagaaacgac
      661 cccaccaccc gtcctgcctc cggtgaccat caatcccaat ccagaacttg tacagcagat
      721 tgaaacactg aaagccgacc gggaccgcga gcgaatccaa cgcgaacagg ctgaaaaaca
      781 attagctgaa ctgaccagta ccgttacccg cttgaaaatc aaaaataaaa agccgggcgg
      841 ggtcgcccct gaaggtgatc aaacccgaac agcagattcg acgagtggaa atacaccggt
      901 aaccacgatt ccgtccaacc tgaccctgcc agcattagaa gatgacctgt tatggcaatt
      961 tatttccaac agtcagaaac ccgaagattt tcttgggtat ctcaagctct atccttcggg
     1021 taaacacgct gacgaagccc gcgccgcact ggctgatttg cgtccccagt tgttaagtga
     1081 ggctcataat acccttggca tcagtcattt tcgactgggt gaatatgacc ctgcactgac
     1141 tgagtttcac aaagcacttg agcttcgccc gcagtttcct gaagcgcaat ttaatctcgg
     1201 gttaaccctg gtgaagcttg gccgattgga cgaagctcaa aaactggtat caccgatcaa
     1261 acaggcgaaa ccttcgctgg ctcgccaact tgaggccgag ctccgcaaag cccgcaagaa
     1321 ctgatttttg tcagtcgtca gtacaccaac tcaaaatctt gaacaggtga gttctcctta
     1381 taagaaaact ctcataagta gtttattgag aatagaatac acttgacaaa gaatttgaat
     1441 ttctaacact tgacgactga cgactgacga ctgactattg actactgacg actaacaacc
     1501 gacttgatcc tctcaatgta atcaaagtcc tcagttgaga gcggtccaaa atccagcgct
     1561 ttcagatttt cttccacctg ggcaacggtt ttaaagcctg gaatagggat ggcccgtggg
     1621 tgacgcgccc aaatccaggc cagcgctccc tgggtcagag tgcgacctcc tcgggtgaga
     1681 atatcttgca ggtgattgat tttattgagg atctgagcct gtttcccatc tttgagattc
     1741 cacccaaacc gtcgccgcat atcattttca gcaaagcttg atccggcatc atatttccca
     1801 gtcagcaatc ccatgccaag cggacgacgg gcaatgcagg ccaggtcaaa ttcgtcacac
     1861 aggtccaggg tttgttgatt ggcgtcaacc aggttgagca attgcggaga ggcagtgcag
     1921 tggtcacctg ccgcaaacag tcgaatccga tccgggtctt cagtggccca ggcataaaat
     1981 ctgatttttc cctgggcgac gagagattcg agggtatcgc ggacttcagc cgcccgcac
//
LOCUS       DAOQDO010001231         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54053_length_2038_cov_3.974786, whole genome shotgun sequence.
ACCESSION   DAOQDO010001231 DAOQDO010000000
VERSION     DAOQDO010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54053_length_2038_cov_3.974786"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKZ53_17560"
     CDS             <1..846
                     /locus_tag="PKZ53_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HNJ42299.1"
                     /translation="PSEKAFLVSSVRKRKVFKISQDGKVSDFLTSGQDGLWGVFGMKV
                     DPKRNRLWLCTSVVREIEGFKAEEDGQAGVFQYDLKTHTLVKKYILPNSEQGHVFGDL
                     ELHPKGDVYITDSKSGGLYRISAATDTLERFLDTSPFVSPQGMALTPDGKRMFVADYS
                     VGIGILDLATKTFSLVPATPEMLLLGIDGLYLHQNSLIGMQNGILPKRVIRLDLDTKL
                     SRIEKLSVIERGTQHLEDPTLGVIVNHSIYFIANSQWESFDKDGTMFPPDKLNQHIIL
                     KASLK"
     gene            859..2004
                     /locus_tag="PKZ53_17565"
     CDS             859..2004
                     /locus_tag="PKZ53_17565"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ42300.1"
                     /translation="MIVDITEVLRTNRWAKSLALLGIWTIVATFFSLQGAAFYWRRGE
                     TIVFSEIFLPEFTFCLVGAVATPGIIWISSRLRIERQILVPRLLLHLICSLFFSVAWM
                     TIPRLVNQTVLSVPFNVQTLINSVIWGLDRGVMVYWVIILLHHSYVYQRQYFESQLQT
                     SRLETQLVNAQLQSLKMQLQPHFLFNTLNSISTLVLVDPKAAHKMVAQLGDFLRLTLE
                     NSSSQEVSFQRELEFLKCYLAIEQTRFQDRLSVMFDIDPTTQDALVPNLLLQPIVENA
                     IKHGIAPRATPGIISIKATQKTGRLHIEINDDGPGLSRLPQSNRKPKGIGLHNTRERL
                     EQLYGDQYRLELVDIPQGGLSVVVEFPLTSTLGSQTAGLVYERSLVV"
BASE COUNT          505 a          499 c          486 g          548 t
ORIGIN      
        1 ccctctgaaa aagcatttct ggtcagcagt gttcgcaaac gcaaagtctt taaaatcagc
       61 caggacggca aggtttctga ctttctcacc agtggtcagg atgggctatg gggtgtattt
      121 gggatgaaag ttgaccccaa acgcaaccga ctctggctct gcacgtcagt cgtacgcgaa
      181 attgaagggt ttaaagctga agaagacggt caggcaggtg tttttcaata tgatttgaaa
      241 acccatacac tcgtgaagaa atatatcctg cccaactctg aacaaggtca cgtttttggc
      301 gacctggaat tgcacccgaa aggcgacgtg tatatcaccg acagtaaatc gggcggactc
      361 tatcgtatct cagccgccac cgatacgctt gaacgatttc tggacacatc tccttttgtt
      421 tctccccagg gtatggcgtt aacacctgat ggcaaacgga tgtttgtcgc cgattattca
      481 gttggaattg gcatccttga tctggcaacc aaaacttttt cgctggtgcc ggcgaccccg
      541 gaaatgctac tgctggggat tgacggactg tacctgcatc agaattcgct gattgggatg
      601 caaaatggaa ttttgccgaa acgtgtgatt cggcttgatc tggataccaa actgagccgg
      661 attgaaaaat tgtctgtaat cgaacgtgga acccagcatt tggaagatcc aacgctcggg
      721 gtcatcgtca atcattcaat ttactttatt gccaacagcc agtgggagag ttttgacaaa
      781 gatggaacaa tgttccctcc tgataaactc aaccagcaca ttatcttaaa agcgtctctc
      841 aaataactgg caggtcttgt gatcgtagac ataactgaag ttttaagaac caaccgatgg
      901 gccaaaagcc tggccttgct gggcatttgg accatcgtcg ccacattctt ttcattgcaa
      961 ggcgccgcct tttactggcg acgcggtgaa acgattgtct tttctgagat tttcctgcca
     1021 gagtttacct tttgtcttgt cggagccgtt gcaaccccag ggattatctg gatttcaagc
     1081 cgtttgcgga ttgagcgtca gattctggtg ccgcgactgc tcttgcatct gatctgtagc
     1141 ctgtttttct cggttgcctg gatgacgatt ccacggctgg taaaccagac ggtgctgagc
     1201 gttccgttta acgtgcaaac actcatcaat tcagtaattt gggggctgga tcgcggcgtg
     1261 atggtgtatt gggtcattat tttattgcac cattcctatg tctatcagcg ccagtatttt
     1321 gaatctcaat tacaaacgtc acgactggaa acccagctcg tcaacgccca gttgcaatcg
     1381 ctcaaaatgc agttacagcc gcattttctg tttaacacgc tcaattcaat ctcgacactg
     1441 gtgctcgttg atccgaaggc ggcccataaa atggtggctc aactggggga tttcctgcgg
     1501 ttgacccttg aaaattccag ctcccaggaa gtctcgtttc aacgggaatt agaatttttg
     1561 aaatgttacc tggcgattga acaaacccgg tttcaggatc ggttgtcggt tatgtttgac
     1621 attgatccga ccacccagga tgcactggtt ccaaatctgc tgcttcagcc gattgtcgaa
     1681 aatgccatca aacacggcat tgctccgcgt gccacaccag gaatcatttc gatcaaggcc
     1741 acccagaaaa ctggccgtct gcacatcgaa atcaatgatg atggacctgg cctctcacgc
     1801 ctcccccaat ccaatcgaaa acccaaagga attggcctgc acaacactcg tgaacgcctg
     1861 gagcaactct atggcgacca gtatcggctt gaactggttg atattccaca gggtggtctt
     1921 tcagtcgtgg tcgaatttcc ccttacctca acattggggt cccagactgc cgggttagtc
     1981 tatgaacgga gtttagttgt atgaaaatcc aagcggtgat tgcagatgat gaaccttt
//
LOCUS       DAOQDO010001232         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54067_length_2038_cov_2.910741, whole genome shotgun sequence.
ACCESSION   DAOQDO010001232 DAOQDO010000000
VERSION     DAOQDO010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54067_length_2038_cov_2.910741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKZ53_17570"
     CDS             complement(<1..366)
                     /locus_tag="PKZ53_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42301.1"
                     /translation="METPIRVYVGATEEHLVPCAVLEYSIRKHTASPVTVLPLYQSGI
                     DIPIPKDPANRPFTKFSFQRFVIPEIQSFSGRAIYLDSDMLVFRDIRQLWEHPFDGAH
                     LLAVQHPEKKQIVQFSVLVL"
     gene            complement(532..>2038)
                     /locus_tag="PKZ53_17575"
     CDS             complement(532..>2038)
                     /locus_tag="PKZ53_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42302.1"
                     /translation="SLVKEQLQASGIPAPFISVTGSLQYEFPPEPSQPSRNRVCAELG
                     ISPESEIVVAPQVHPGEEQLLCEAVRYLRRTRPSIKLVLVVYSDAQLRDIQRLLSRAG
                     MKNHVAGEPGANPESEVVICSTSFDSSVLAYLAQVVIAGGSFVPNARTYNPIAAVQLR
                     KPVVAGPYLTPLTAATRQLLHISAIQSIDQKTLADTLEAVLSHPETYQTRLDSAHTFV
                     QNLNGAAGKLFGLIESSLEKPSPQLPARNRLAPTFRDQIGQSRAWKLLAQPLTRRQIP
                     DLDALRQRLGNPDTILCLGNGPSSEDPAVFEVQYDALFRVNHLWQQRGRLIHPDVVFV
                     GTPETLKKVRPCIYGFSSVRKEYGMLLRNLWTSGPQPIEYCTVERLCNFQEYSRTDKL
                     SNGTLMVMVAAALRPKRLIIGGIDLYRHPDGRYPGDFHATNNYAPTHDRNIELDVITA
                     TLREFPNELVIIGDVLHQSLIESGINLGLKKPGPGAEGLGLKTRKLGQSKG"
BASE COUNT          512 a          506 c          541 g          479 t
ORIGIN      
        1 cagcaccagc acgctaaact gaactatttg cttcttttca gggtgttgaa ccgcgagcaa
       61 atgtgcccca tcaaatgggt gctcccacaa ttggcgaata tcacggaaca ccagcatgtc
      121 tgaatcaaga taaattgccc gtccggaaaa tgactgaatc tcggggatca caaatcgctg
      181 aaaagaaaac ttggtgaacg gacgattggc cggatctttt ggaattggaa tatcaatccc
      241 gctttgatag agcggtaaga ccgtaactgg cgaagcggta tgtttccgaa tagagtactc
      301 aagcaccgca cagggcacca gatgttcttc agttgcaccg acatacacgc gaatcggagt
      361 ttccatataa gttctcgggc tgaagatatt gggttgaaga catcgggctg aagaagtcgg
      421 gctgaagata ttgggttgaa gacatcgggc tgaagaagtc gggctgaaga agtcgggctg
      481 aagaattggt tttctttcat ccttcatccc ctcatccctt gggttcatcc ctcatccctt
      541 tgattgcccg agcttgcgag tcttcagccc caagccctca gccccaggcc ctggtttttt
      601 cagccctaag ttgattccgg attcaatcag cgactggtgc aacacatcac caataatgac
      661 cagttcattg ggaaattcgc gcaaggtagc cgtgatgaca tccagttcga tattgcgatc
      721 atgtgtcggg gcataattgt tagtcgcatg aaaatcaccc ggatagcgcc catctggatg
      781 ccggtacagg tcaattccgc cgatgatcag tcgtttcggg cgtaaggcag ccgctaccat
      841 caccatcagg gtgccgttgg aaagcttatc ggttcgactg tattcctgaa agttgcacaa
      901 tcgctcgaca gtacaatatt caatcggctg tggtccagat gtccacaaat tgcgcaagag
      961 catgccgtat tctttgcgca ccgaagaaaa gccgtaaatg caaggtctga ctttcttgag
     1021 cgtctctgga gttcccacga aaaccacatc cggatggatc aatcgtccac gctgttgcca
     1081 gagatgattg acccgaaaca gagcatcata ctggacctca aaaaccgccg ggtcttctga
     1141 tgatggaccg ttgccaagac acaaaatggt gtccgggttt ccaagtcgtt gacggagcgc
     1201 atccagatca gggatttgcc gccgggtcag aggctgggcc agcaatttcc acgcccggct
     1261 ttgcccgatt tggtcgcgga acgtgggggc aagtctattt cttgccggta actggggcga
     1321 aggtttctca agtgaggatt caatcaaacc aaagagtttc ccagccgcac cattcaggtt
     1381 ttggacaaac gtatgggctg aatccagccg ggtctgatag gtttcaggat gactgaggac
     1441 agcctccaat gtatcggcaa gtgttttttg atcaatggat tgaatggcgc ttatgtgcag
     1501 gagttgacgc gtcgccgctg tcagtggtgt cagatatggt cctgccacga ctggtttcct
     1561 gagttgcacg gcagcgattg gattataggt cctggcgttt ggaacaaaac ttccacccgc
     1621 aatcacaacc tgagccagat aagcgaggac gctcgaatca aaggaagttg agcagatgac
     1681 cacttctgat tcaggattgg cgccaggttc gccggccaca tgatttttca taccagccct
     1741 ggaaagcaga cgttgaatat cccgaagttg cgcgtcggaa tacaccacaa gcaccagttt
     1801 gatagacggt cgggtccggc gaaggtaacg caccgcctcg cacaagagtt gttcctcacc
     1861 tggatgaact tgcggtgcca caacaatctc agattcaggt gaaatcccaa gctcagcaca
     1921 tacccgattg cgggaaggtt gagacggttc tgggggaaat tcatactgca gactgccagt
     1981 gactgaaatg aacggggcgg ggatgcccga tgcctgaagt tgctctttca ccagggat
//
LOCUS       DAOQDO010001233         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54175_length_2036_cov_2.979303, whole genome shotgun sequence.
ACCESSION   DAOQDO010001233 DAOQDO010000000
VERSION     DAOQDO010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54175_length_2036_cov_2.979303"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKZ53_17580"
     CDS             <1..1179
                     /locus_tag="PKZ53_17580"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ42303.1"
                     /translation="VQPPSVQAGPDQTVCTGTESIQLNGSLSGVSQATWTGGAGTFSP
                     NATALNATYFPTSSGMESGSVTLMLISEDPAGPCTAVSDNMTISFVDCVERSVVYVPD
                     TLNNRIQLFDGQQWSVVAGGVVGSGNGQFRSPEAVAVSASRLKLYVADTGNNRIQWST
                     DGGATWADFATVGSAPNQVRAPEGLALDALGNLYVSDTGNGRVLRFNNGVPGMGVVIS
                     SNGQGSGQIVSPRGMTIDSEFTLYVVDEANSRILKITNANLVTISTTGTILSAKGVGL
                     NHVQNPQGIAVDSFGNLYIADTGNSRILKFPNGNPANGVALALTGSQLGQVNRPEGVT
                     VCRFTSGPQAGAQFLVIGDTLNNRIQGRLVSGGPWSLVGLPNGAGTTVGRFRGPSKIR
                     "
     gene            complement(1411..1794)
                     /locus_tag="PKZ53_17585"
     CDS             complement(1411..1794)
                     /locus_tag="PKZ53_17585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNJ42304.1"
                     /translation="MNFLDLEDILELHLQAIERFGGSAAIRDQGLIESALAAPQNLSF
                     YEDADAIACAAGYGYALCQNHPCVDGNKRIAAAALELFLLLHGWTLHHVSDEQLEGLI
                     FAVASHSIDRSDFEARLRALAQPIE"
     gene            complement(1791..>2036)
                     /locus_tag="PKZ53_17590"
     CDS             complement(1791..>2036)
                     /locus_tag="PKZ53_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42305.1"
                     /translation="TAEAARQQAEHGGLEPWFIFWIPTEERQTPPISTQRQIPQLTDA
                     ERKARLAALAEDYFTRRDELMKRLAAGVDEAQEEGTE"
BASE COUNT          467 a          508 c          577 g          484 t
ORIGIN      
        1 gtccaaccgc catcagttca ggctggacca gaccagacag tttgtaccgg gacggaatct
       61 attcaactga atggaagtct gagtggggtc agccaggcga cctggaccgg cggcgctgga
      121 acgttttcac cgaatgccac cgccctcaat gccacctatt ttccaacttc ctcaggaatg
      181 gaaagcggga gcgtgacatt gatgctgatt tctgaagacc cagccggccc ctgcaccgcc
      241 gtgagcgaca atatgacaat ttcctttgtt gactgtgttg aacggtcagt cgtgtacgtt
      301 ccagacacac tgaacaaccg cattcaactg tttgacggcc agcaatggtc agtggtcgct
      361 ggtggtgtcg tcggctccgg caacgggcaa tttcgcagcc ccgaagctgt tgccgtgagt
      421 gccagccgcc tgaaactcta tgtcgccgat accggaaaca accggattca atggtcaacg
      481 gatggcggcg caacctgggc tgattttgcc acggtgggaa gcgctcccaa tcaggtccgg
      541 gcaccggaag ggcttgcgct ggatgcactt gggaatttgt atgtctcaga taccggaaac
      601 ggtcgtgtcc ttcgatttaa caatggtgtt cctggaatgg gcgtggtgat ttccagtaac
      661 ggacagggaa gcggccagat tgtcagtcca cgcgggatga cgattgatag cgagttcacc
      721 ctgtatgtcg tggatgaagc caacagtcga attctgaaaa tcacaaacgc caatctggtg
      781 accatctcga ccacgggcac gattctttca gccaaaggcg ttggattaaa tcacgttcaa
      841 aatccgcagg gaattgcggt tgattcgttc gggaatctct acattgcgga cacaggcaat
      901 tcgcgcattc tgaaatttcc caatggaaac ccggccaatg gtgttgcgct ggcgctcact
      961 ggttcacaac ttgggcaagt caatcgtcct gaaggcgtta ccgtctgccg ttttaccagt
     1021 ggaccccagg ccggcgctca gtttttggtg atcggtgaca ccctcaacaa tcgcattcaa
     1081 gggcgactgg tgtcaggcgg gccctggtca ctggttggtc tgccaaatgg tgccggcacg
     1141 acggttgggc ggtttcgcgg accgagcaaa atacggtgac agaggtcagg gttcagggtt
     1201 cagggttcag ggttttcgaa cgttgattgg ctgaagtctt tgccgtggag cggcggttga
     1261 attgaggcag gtcggttacg gcctgcctct caacgtgtgg gctggtcata tccatatgga
     1321 gtagaatccc tgtactagaa aatagtcaaa taattcaatt aaatcagctt agcgaactac
     1381 tgattactga ctactgacta caaactggta tcattcaatc ggttgggcta aagcccggag
     1441 ccgggcttcg aaatcactcc gatcaattga atggctggcc acagcaaaaa tgagtccttc
     1501 caattgctcg tctgaaacgt ggtgcaacgt ccagccgtgc agcaaaagaa aaagttcgag
     1561 tgcggcggcg gcaatgcgtt tgtttccatc aacacagggg tgattttgac agagggcata
     1621 cccgtacccg gcggcacagg caatggcgtc ggcatcttca taaaaagaaa gattttgtgg
     1681 tgctgccaga gctgattcaa tcagtccttg atcgcggatg gccgctgagc ctccaaatcg
     1741 ttcaattgcc tggaggtgaa gctctaaaat atcttccaaa tcaagaaaat tcattcggtg
     1801 ccttcttcct gagcttcatc aactccggcg gcgagtcgct tcatcagttc atcgcggcgg
     1861 gtgaaatagt cttcagccag tgcggcaagt ctcgctttgc gttcagcgtc ggtgagttgt
     1921 ggaatctgac gttgggttga aatgggcgga gtttgacgtt cttcagttgg aatccagaag
     1981 ataaaccagg gttcaaggcc accgtgttct gcctgttggc gtgcggcttc agccgt
//
LOCUS       DAOQDO010001234         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54219_length_2035_cov_2.995960, whole genome shotgun sequence.
ACCESSION   DAOQDO010001234 DAOQDO010000000
VERSION     DAOQDO010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54219_length_2035_cov_2.995960"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>2035)
                     /locus_tag="PKZ53_17595"
     CDS             complement(223..>2035)
                     /locus_tag="PKZ53_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ42306.1"
                     /translation="ELFLTFFFLLVGFYHFQLYAGRRQLTEYLWFGVFAIGFGINTFC
                     NTAWALEITSPFLADVTKVFLRHLLVIAYVRFMGYLLSCPTKWWLPALEEVQLVCSAI
                     VLFWPEVVLTQVNHWMFLSIVPIMVRFLIFTAQEAWRGHPDARTMVFGLIAVTVIETL
                     TIARAFGLIQIPFIAPWAMAALIISMAVALTNRFNRVYTELDSLNQELESKVADRTVA
                     LAETVAQLQEAQVQTERKVQEISQKNQELIASQQQADRIFSALAEALPGTVLDEKYRL
                     EEKIGEGGFGVVFRGTHLSLGRAIAVKVFKPRPGNDNADAVERFKREGISVSRLTHPN
                     VINVLDSGISTQGIAYMVMELLSGKSLEADLKTRTVTTLRHCLEVIIPVCHALAEAHR
                     LGIIHRDIKPDNIFLNQTPEGEVIKVVDFGIAKMVGDEGDENYRKLTMTNSLIGTPVY
                     MSPERVAAKPYDGRSDVYSVGIVLFELLTGRPPFERDLSGLVGLMLAHMNDAPPSICS
                     LNPQIPPIIETIVLKTLEKNPDDRPTAKELAQLLIQALKGIPLAEQNIQFTRLVPEST
                     FDTTQSRVVSDMENAATRGNSLSERPTQIDTPEVTPS"
BASE COUNT          514 a          525 c          524 g          472 t
ORIGIN      
        1 agcggatttg aaccgctgac ccctaccgtg tcaaggtagt gctctacccc tgagctacgc
       61 gtctatccgt gggtgttggt cattgatact ctggccgaat cgccaaagta gcgggcaacc
      121 ttagcaaaca gtccctaagg tgtcaagggg gtaaccgctc aaggttcaaa gttcagggtt
      181 tgggttcatc ccattcgtcc gttggttttt cctaaatcct ggtcaagatg gcgtgacctc
      241 aggtgtatca atctgggttg gccgctcaga aagagagttc cctctggtcg cggcattttc
      301 catatcactg accacccggc tctgagttgt atcaaatgtt gactcaggga ccagtcgggt
      361 aaactggatg ttttgttcag ccagtggaat tcccttcaag gcctggatca gcagttgggc
      421 cagttctttg gcggttggcc gatcatcggg atttttttca agcgttttca agacaatggt
      481 ttcaatgatc ggtggaattt gtggattgag tgaacaaatt gacggtggag catcattcat
      541 atgagccagc atcagtccaa ccagcccgct taaatcacgc tcaaacggag gccgtccggt
      601 gaggagttca aagagtacga tgcctacgct gtagacatct gagcgtccat catagggttt
      661 ggccgctacc cgctcgggtg acatgtacac cggagtgcca atcaggctgt tggtcatagt
      721 gagttttcga taattctcgt caccttcatc gccaaccatt ttggcaatcc caaaatccac
      781 caccttgatc acctcgcctt caggcgtctg gttgagaaaa atgttgtcgg gtttgatatc
      841 acggtgaatg atccccaggc gatgggcttc ggccagggca tggcacactg gaatgatgac
      901 ttccagacaa tgccgcaacg tggtgacagt tcgtgtcttg agatcggctt ccagtgattt
      961 tccactcaga agttccatga ccatataggc aatgccctga gtcgaaatcc cagagtcaag
     1021 cacattgatc acattgggat gggtcaaccg tgagaccgaa ataccttcgc gtttaaagcg
     1081 ttcgaccgca tcggcattgt cgttgccagg ccggggttta aatactttca ccgcaatggc
     1141 tcgcccgagg ctgagatgtg tcccgcgaaa gacgacccca aagccgcctt cgccaatttt
     1201 ttcttccagc cggtattttt catccaacac cgtaccgggc agagcctcgg ccagggctga
     1261 gaaaatccga tcagcctgct gttgtgaggc gatcaattcc tgatttttct gggagatttc
     1321 ctgaaccttg cgctcggtct gaacctgggc ttcctgcaac tgggccacgg tttcggccag
     1381 ggccaccgtc cggtcagcca ccttgctttc cagttcctga ttgagtgaat ccagttcggt
     1441 atagacgcga ttaaatcggt ttgtcagagc cacagccatc gagatgatca aggccgccat
     1501 cgcccacggc gcaataaacg gaatctgaat gagcccaaag gcgcgagcaa tcgtgagcgt
     1561 ttcaatcacg gtgaccgcaa tgagtccaaa caccatggtc cgggcatcag gatgaccacg
     1621 ccaggcttcc tgggcagtaa agatcaagaa ccggaccata atcgggacaa tggataaaaa
     1681 catccagtga ttcacctggg tcagaaccac ctccggccag aagagaacaa tggctgaaca
     1741 gaccagttgg acttcttcga gcgccggtag ccaccacttc gtgggacagg acagcaaata
     1801 tcccataaac cggacatagg caatcaccag caggtgacgt aaaaacactt tggtgacatc
     1861 tgccagaaag ggagaggtga tttcgagggc ccaggctgta ttacaaaagg tgttaatccc
     1921 aaaaccaatg gcaaaaacgc caaaccaaag atattcagtc agttgtcgcc tgccagcata
     1981 taattggaag tgatagaaac caaccaggag aaaaaagaaa gtcagaaaca actcg
//
LOCUS       DAOQDO010001235         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54253_length_2034_cov_4.202122, whole genome shotgun sequence.
ACCESSION   DAOQDO010001235 DAOQDO010000000
VERSION     DAOQDO010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54253_length_2034_cov_4.202122"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2034
                     /locus_tag="PKZ53_17600"
     CDS             <1..>2034
                     /locus_tag="PKZ53_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNJ42307.1"
                     /translation="DELHTYDGAQGADVACLLRRLKERLNISKGELCVIGTSATLDNR
                     EPGARLEGGTIDGKETPNDRLARFASTIFEEDIAADAVIIEDRLTVEEIVHADLHDTY
                     LPDPGHCVPLEDEDSFQYAVRQSKLWGGPQFFNSPLISIEPAQGTSENIENRHVVVEK
                     WSVRLGSWLKGQKLFSDLLTVFSEAETQGKGLLTWNELIEHLSQADLGFIGYQQFSDR
                     QHICASFLALVSQAREERNGYAFPMIPIQVQIWIRELRRLGRLVHENPIFAWLDEPTQ
                     EYPVLPTFHCLECGESGWIALHDRSKDASLQATGVEGIQLDSDPTRIYRSWFGQKGQR
                     SAHLVVISPFREASQTGEAQPNLFDVWQLHPKSLVLRHGEGHCPLTYDPKSIKVRINK
                     QAKKDEISGTVSGVQGCPQCKTKESVFFIGSQSATLSSVALDELFGSILNDDPKLLAF
                     TDSVQDASHRAGFFTARTYQFTFRTALQRVVESTGSNGLPLVDAGKAVLDWWAHPRPG
                     WPGHISEAIAALLPSDLHQYQPFLKYRQQQSKEALSPHLLAEIQERLTWEATSEFGLR
                     QSIGRTLEMAGSACLGWDEAILTSTLQRLRERFANQHPLFAKVPSDLWRLWLYGFLHR
                     ARSRGALSHRFLTDFARKDFWGKHPFGKTIKEREIYPPARHSRPRLLVTLPHNG"
BASE COUNT          517 a          490 c          524 g          503 t
ORIGIN      
        1 gacgaacttc acacctatga cggggcgcag ggagcagatg tcgcctgttt gttaagacgg
       61 ctcaaggagc ggttgaacat ttccaaaggt gagctatgtg ttattggaac aagtgctacc
      121 ctggataatc gtgagccagg tgcccgactg gagggaggta ccattgatgg gaaagaaacc
      181 ccaaatgacc gtttagcaag gtttgcaagc acgatctttg aggaagatat cgccgcggat
      241 gcagtaatta ttgaagatcg gcttacggtt gaagaaattg tccatgctga cctgcacgac
      301 acctacctcc ccgatcctgg tcactgtgtc ccgcttgagg atgaggacag ttttcaatat
      361 gccgtccggc agtcaaaatt gtgggggggg cctcaatttt tcaattcccc tttgatttca
      421 atagaaccag cccaggggac cagcgagaat atagagaatc gtcatgtagt ggtagagaag
      481 tggtcagttc gtcttgggag ctggctaaaa ggacaaaagt tattttctga cttgctgacc
      541 gtgtttagtg aggctgaaac tcagggcaaa ggattactta cctggaatga attaatagag
      601 cacctctcgc aggcagattt gggctttatt gggtaccaac aattttctga tcgccagcat
      661 atttgtgctt cttttttggc tttggtgtca caggctcgtg aagaacgaaa tggctatgcc
      721 tttccaatga tcccgattca agttcaaatc tggattcggg aattaagacg gttgggcagg
      781 ttggtccacg aaaatccaat ctttgcctgg ttggatgaac caactcagga atatccagta
      841 ctgccgactt ttcattgcct ggagtgcggt gagtctgggt ggattgcatt gcatgaccgg
      901 agcaaagatg catccctgca ggcaactggg gttgaaggaa ttcagcttga cagcgaccca
      961 acccgaattt atcgaagctg gtttgggcaa aaaggccaac gtagtgccca tctggttgta
     1021 atcagtccgt ttcgggaggc ttctcaaact ggcgaggcgc aacccaacct gtttgacgta
     1081 tggcaacttc acccaaaaag cctggtcctc aggcacggtg agggccactg ccctctcacc
     1141 tacgacccga aatcaatcaa ggtccggatc aataaacaag ccaaaaaaga cgaaataagt
     1201 ggtacggtat ctggtgtgca gggatgtccc caatgtaaaa ccaaagagag tgttttcttt
     1261 attgggagcc aatccgccac tttgtcgagt gtggctcttg atgaattgtt tggctcaatt
     1321 ctcaatgatg accccaaact gctagccttc accgatagtg ttcaggatgc ttcgcaccga
     1381 gccggttttt ttacggctcg gacctatcaa ttcacattcc gcacagcatt gcaacgagtc
     1441 gtcgaatcga ctggaagcaa cgggttacca cttgtggatg caggaaaagc ggtgctcgat
     1501 tggtgggcac acccgcgacc tgggtggcct ggacatataa gcgaggcaat tgcggctttg
     1561 ttgccatcag atttgcacca atatcagcct tttttaaaat atcgccagca gcagagcaaa
     1621 gaggctctgt caccacattt actggccgag attcaagaac gtctgacctg ggaagccacc
     1681 agtgaattcg gacttcggca atccatcggg cggacattgg agatggcggg aagtgcctgt
     1741 cttgggtggg atgaagccat acttacttca accttgcaac gcctccgaga acggtttgcc
     1801 aaccagcacc ccctatttgc caaagttcct tcagacttat ggcggctttg gctctatggt
     1861 tttctacacc gagcccgatc acgtggggcc ttgtcacatc gattcctcac cgattttgct
     1921 cgcaaagact tctggggaaa acatccattt ggcaaaacca ttaaagaacg agaaatctat
     1981 ccgccagcca gacattcacg cccacggcta ctggtcaccc tacctcacaa tggt
//
LOCUS       DAOQDO010001236         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54399_length_2031_cov_2.793016, whole genome shotgun sequence.
ACCESSION   DAOQDO010001236 DAOQDO010000000
VERSION     DAOQDO010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54399_length_2031_cov_2.793016"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..>2031)
                     /locus_tag="PKZ53_17605"
     CDS             complement(300..>2031)
                     /locus_tag="PKZ53_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNJ42308.1"
                     /translation="DYALAREVFNADSEKIRELRAWLMKPTGEVVKYDKKEIVELAMV
                     DNDIYNEARVKMISPGVEADAGCVFGFESITEQNSVFSQFIWDFQHRLPVVASRCSLT
                     TPAGWRTESVMFNHAKVEPRTSGTTTTWELTGLPGLPSEPASPEVTTMAPRIAISVFP
                     PAGSSSGSLRTFASWSDVSRYLSELCDPQAAVTDVITTKAREITAGAKTEQERIAAIA
                     RFAQKIRYISIQIGVGRGGGYRPHAAQDVLTKAYGDCKDKANLMRAMLKAVNIPAYPV
                     VIYAGDPTYVREEWPSPHQFNHCIVGVKVSDETKLETVVQHPGLGRILIFDPTDEFTP
                     IGDLPQHEQGSLALVVAGEAGALLRMPQTPPEANSTDRTTQVKMSNTGVAQASLIELA
                     SGQAAVEYRGEFRSQPLSEYQKIIESWIGGTFSGASVASVKPNDDPSGGKFQLHLEFT
                     APNFGQLVQGKLLMFKPAMLTLTKKYRLTSQFRMHPVVIEPESFRETATFHVPDGFAV
                     DEIPDPIKLEAPFGSYTATCEVKGTEIRYSRSLLLKSSTVPASDYEAVKGFFTKVKTA
                     EQSAVVLIAR"
BASE COUNT          479 a          542 c          514 g          496 t
ORIGIN      
        1 acaaatccca tccccaacca gacaaagcct tttttcaatt tcgcaatcat ggaacaaaat
       61 ctccttccag gttcagctta taccagttgg ggctgagggt atcgggctac agcatcgtta
      121 aaagataact tcctgttttt gttgacggac acaggttcag agagcatttc aaatcaggaa
      181 accacgaaaa acacgaaaaa gaagtccaaa tttttcaatg gtttccgaga tgtagccagg
      241 tcaaacgcaa gggaaatatt tatctgaaaa gctatagccc gcctgacccc tcagtggatt
      301 tacctggcaa tcaacaccac ggcggattgt tcggcggttt tgacctttgt aaaaaatcct
      361 ttcacggctt catagtcaga agccgggacc gtgctggatt tcaagagcaa tgatcgcgaa
      421 tagcggattt ccgtaccttt cacttcgcag gtcgcggtat agctgccaaa cggagcttcg
      481 agcttgatcg ggtcagggat ttcatctacc gcaaacccat ctgggacgtg aaacgtcgcc
      541 gtttcccgaa aactttccgg ttcaatgaca accgggtgca ttcggaactg actggtcagc
      601 cgatattttt tggtcagggt cagcatggcc ggtttgaaca tcaataattt cccctgcacc
      661 agttgcccaa aattcggcgc cgtaaactct aaatgcaact ggaattttcc gcctgatggg
      721 tcgtcatttg gtttgacact ggccacactg gcacctgaaa acgtgccgcc gatccaggat
      781 tcgataattt tttgatattc cgacagtggc tggctgcgaa attctccgcg atactcaacc
      841 gcagcctgac cactggccag ttcaatcaaa ctggcctggg caacgccggt gtttgacatt
      901 ttgacctgcg tggttcggtc agtgctattg gcttcaggag gagtctgcgg cattcgcagc
      961 aaggccccag cctcacccgc caccaccagc gccaggctcc cctgttcgtg ttgcggcagg
     1021 tcgccaatcg gcgtgaactc gtcagttggg tcgaaaatta aaatccggcc cagacctggg
     1081 tgctggacga cggtttccaa tttggtttcg tccgaaactt taaccccgac aatgcaatgg
     1141 ttgaattgat gcggagacgg ccattcttca cggacatagg ttgggtcacc ggcataaatc
     1201 accactggat aagccgggat attgacggct ttgagcatcg cccgcatcag attggctttg
     1261 tctttgcaat cgccataggc tttggtgaga acgtcctgag ccgcatgcgg tcggtatccg
     1321 ccgccgcgtc cgacgccaat ctggatcgaa atatagcgaa ttttctgggc aaaccgggca
     1381 atggcggcaa tgcgttcctg ttcagttttg gcacccgctg tgatctctcg ggctttggtg
     1441 gtaatcacgt cagtcacggc ggcttgcggg tcacacagtt ctgaaaggta gcgcgaaaca
     1501 tctgaccagc tggcaaacgt ccgcaacgaa ccagagctac ttccggcagg cggaaaaacg
     1561 ctaatggcga tacgcggggc catagtggtg acttccgggc tggctggctc ggagggcagc
     1621 cccggcagcc cggtcagttc ccaggtcgtg gtggtgcccg aagtgcgtgg ttcaactttg
     1681 gcgtgattaa acatcacact ttcggttctc cacccggcgg gtgtggtcag cgaacaccgt
     1741 gacgccacca ctggcaaccg atgctgaaag tcccagataa attgagaaaa cactgaattc
     1801 tgttcggtaa ttgattcaaa cccgaagaca cacccggcat cagcctcaac tcccggtgaa
     1861 atcattttca cacgggcttc gttgtaaata tcgttgtcaa ccatggccag ttcgacaatt
     1921 tcttttttgt catatttgac cacctcgccg gttggtttca tcaaccaggc tcgaagttca
     1981 cgaatttttt cgctgtcagc attaaatact tcgcgcgcca gggcatagtc t
//
LOCUS       DAOQDO010001237         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54556_length_2028_cov_2.886974, whole genome shotgun sequence.
ACCESSION   DAOQDO010001237 DAOQDO010000000
VERSION     DAOQDO010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54556_length_2028_cov_2.886974"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2028
                     /locus_tag="PKZ53_17610"
     CDS             <1..>2028
                     /locus_tag="PKZ53_17610"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ42309.1"
                     /translation="GTDQGLPHQSMNDLLEAQDGIYWVATNGGGVGRFDPAVKSEFVF
                     GSGVTRSQQLTRQLFVSIPVGDESPTNRVNVVFQSQDGRIWAGSDKGLFVLVETPATR
                     TFQLVDLRPDLPATAFLQIWAIVEDTDGSLWIGTSHGLTRLFQDGHIIVYQLPPPAAD
                     VVVWSVLKDRDGRIWVGSNRGVSVIIPAAESAMGSPTRLSLKPVTPLALSSQTKNFVL
                     PATQPNGLTFAKENGLPDSVVRSLFQASDGTIWIGTRTGAATFCTGQLQQCVGAAPIT
                     NQLILTLAEDSAHNLWLGTETSGAFRIAHDGFSAFTKDDGLEDPEIRTVLLDPAGRVV
                     GVGNGMLQVLTGQRFTTVRPLVPAAENKDGKTRYRAFLLDHTGAWWAATTKALYRFPK
                     VDTTESLATARPTKICSTDDGLALMTLLKLVEDQDGNLWILMTGQQPLARWERATEKL
                     HIFSDVDGVPPYSTPSALCQDKAGNIWVGLRENGLMRYRQGQFERFTRNDGVPGFEIR
                     SLYLDGSGCLWIGSTAEGAARIDHPEHLRPVAVRYTTRQGLTSDIVRSISADLRGDIY
                     LGTARGVDRLDPHTNRISHYTIADGLNGSEIEASVCDRDGAIWFATRTGLSRYRPPSN
                     VVPAVAQVSITGLKVGGKPKALLEFGESEVSNLSLEPDQNNVQIEFSGLSF"
BASE COUNT          438 a          517 c          573 g          500 t
ORIGIN      
        1 acggcacgga ccagggcctg ccacaccagt caatgaatga cctgctcgaa gcgcaggatg
       61 gcatctactg ggtggcgacc aatggtggcg gcgtcgggcg ttttgacccg gcggtcaaat
      121 cagagtttgt cttcgggtca ggcgtgactc gatctcagca attgactcgc cagctttttg
      181 tcagtattcc cgtcggggac gaatcgccaa ccaatcgcgt caacgtggtg tttcaaagtc
      241 aagatggccg gatttgggca ggttccgaca agggattgtt tgttctggtt gagacccctg
      301 cgacccggac gtttcaactg gttgacctgc gaccagattt acctgccacg gcctttcttc
      361 aaatctgggc catcgttgaa gatacagatg gaagtctgtg gattggaact tcgcacggat
      421 taacgcgcct gttccaggat ggccacatta tcgtgtacca gttaccgccg ccggctgctg
      481 atgtggttgt gtggtcagtg ctcaaagacc gtgatgggcg gatctgggtt gggtcaaacc
      541 gtggagtgtc agtcattatt ccggcggctg agtcagctat gggaagtcca acccggcttt
      601 cactcaagcc agttactccg cttgccctgt caagtcaaac aaaaaatttc gtcctgccag
      661 ctactcagcc caacggtttg acctttgcca aagaaaacgg tttgcctgat tcagttgtca
      721 ggtcactttt tcaggcttcg gatggaacca tctggattgg tacccggact ggcgccgcta
      781 cgttttgtac cggacaactt caacagtgcg tcggtgcggc cccaattacc aatcaactga
      841 ttctgacact ggccgaagat tcagcccata acctctggct tgggaccgag acgagcggtg
      901 cctttcgaat tgctcacgat ggtttttcgg catttaccaa agacgatggc ctggaagacc
      961 ctgaaatccg gactgtgctg cttgatcctg ccgggcgagt ggttggggtt ggcaatggca
     1021 tgctgcaggt tctgaccggt caacggttta caaccgtcag gcccttggtg cccgccgccg
     1081 aaaacaagga tggaaaaacc agataccggg cttttttgtt ggatcacacc ggtgcctggt
     1141 gggcggccac gaccaaagcc ctgtatcggt ttccaaaggt tgataccact gagtcacttg
     1201 ccactgcccg gcccacgaaa atctgttcaa ctgatgatgg actggctttg atgactctgc
     1261 tcaagctggt tgaagatcag gacggtaatt tatggatctt aatgactggc caacagccac
     1321 tggctcgatg ggagcgggca accgagaaac tccatatttt ctcagacgtt gatggagtcc
     1381 ccccatacag cacccccagt gcgctgtgcc aggacaaagc cggaaatatc tgggttgggc
     1441 tgcgtgaaaa tgggttgatg cgctatcggc aagggcaatt tgagcggttt acccggaatg
     1501 atggagtgcc ggggtttgaa attcggtcgc tttacctgga tgggtccggt tgcctgtgga
     1561 ttggttcaac cgctgaaggt gccgcccgaa ttgatcatcc tgagcatctg cgtccagttg
     1621 ctgttcggta cacaacccgt cagggattga ccagtgatat tgttcgctca atttcggctg
     1681 atcttcgcgg cgacatttat ttaggtacgg cacgtggggt ggaccgcctc gatccacata
     1741 ccaatcgaat tagccattac acaattgcag acgggttaaa cgggagtgaa atcgaggctt
     1801 cggtatgcga ccgggacggg gcaatctggt ttgccactcg aaccgggctt tctcgctatc
     1861 gtccgccttc caacgtggtt cctgctgtgg ctcaggtcag cattactggt ttaaaagtcg
     1921 gcggaaaacc aaaggccctg cttgaatttg gcgaatctga agtcagcaat ttaagtttgg
     1981 aaccggacca gaacaacgtg cagattgaat tttcgggatt gtcctttg
//
LOCUS       DAOQDO010001238         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54558_length_2028_cov_2.870248, whole genome shotgun sequence.
ACCESSION   DAOQDO010001238 DAOQDO010000000
VERSION     DAOQDO010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54558_length_2028_cov_2.870248"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1291
                     /locus_tag="PKZ53_17615"
     CDS             206..1291
                     /locus_tag="PKZ53_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNJ42310.1"
                     /translation="MMCRGQKSLCSLFLAVLMLCVSSGFPVWAQSPAPAAQELKVSPV
                     SEVTVESVTAADLGLGSEATSGEIKASTTGFTSHFRNWLATNGYSSYDFARDDVTGGS
                     YGGKSSASDTVVNQPVIFIHGNSDKAIGTGTFGQSGWSASVDYFLSQGYKTSELYATT
                     WGPANAAYSAYQYHSKTNLTRIRKFIEAVLAYTGAAKVDIISHSMGVTLGRKAVKGGS
                     ASDLLEGGSYDLGPSLTSKVDTFVGIAGANWGLVSCYQTGGSTPTCASTNGLYPGYAI
                     GPWGLSSILSDLNATSHYEGSYVYTIWSTVDEVIGYGCVVWGRYTPQIPAQNGEKRYT
                     SAPYGHFNSKDLTTYYQLRMVKYHSTT"
     gene            1410..>2028
                     /locus_tag="PKZ53_17620"
     CDS             1410..>2028
                     /locus_tag="PKZ53_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42311.1"
                     /translation="MPLATLSNSVSLAYDIQGEGPPLVLICGLGTGGWIWCKQVPELA
                     QHFQVITFDPRGVGESSQVAAPFSIADLAEDISFLLEHLGLERAHIVGASLGGIVAQQ
                     FALTFPHLVERLVLSCTTFGGPNHVLPGGEGLAALMSVSGLNGEERARRNIQFGFTKA
                     YLENNMDDVEDIIRMRLACNIPEHVYLFQVKAASEFNAEPHIPAIQ"
BASE COUNT          457 a          535 c          545 g          491 t
ORIGIN      
        1 caggctggct gaagtcttta gccgcgggtt actcaagtct gaccaaccag ggcgccccca
       61 gaactgagcg tgacaccacg cccagacgca ccatgcaagt ttccttttct ctttcgccag
      121 gcaatccaca atcaatttca aactggtgtt gccctttcat ccgtccgtct tccgaaccaa
      181 aaagcaagag gagtgtgtcg aagctatgat gtgccgtggt caaaagtcgc tctgttcact
      241 gtttttagcc gttttaatgc tttgcgtttc atctgggttt ccagtctggg cacagtcacc
      301 agccccagcc gcccaggaac tgaaagtcag cccggtctct gaggtcactg ttgaaagcgt
      361 aacggcggca gaccttggtc ttggctcaga agccaccagt ggtgagatca aagccagcac
      421 cacgggcttt acttcgcact ttcggaactg gcttgcgacc aacgggtata gcagctatga
      481 ctttgcccgc gacgatgtga cgggcggcag ttacggcggc aaatcgagcg ccagcgacac
      541 ggtggtaaat cagccggtga tttttattca cggtaattct gacaaagcga ttggaacggg
      601 cacctttggt caaagcggct ggagcgcgtc ggttgactac tttttgtccc agggctacaa
      661 aacctctgaa ctctatgcca cgacctgggg accagccaac gcggcctatt cagcctatca
      721 gtaccactcc aaaacaaatt tgacccggat tcgcaagttt atcgaagcgg tgctggccta
      781 caccggtgcg gccaaagttg acatcatcag ccattcgatg ggggtgacgc ttggacgcaa
      841 ggctgtgaag ggcggttcag ccagtgacct cctcgaaggt ggttcatacg atttggggcc
      901 atcactgacg agcaaggttg atacctttgt tggaattgcc ggtgctaact ggggattggt
      961 ttcgtgctat caaaccggtg gttcaacccc aacctgtgct tcaaccaacg gcctctaccc
     1021 tgggtatgcg attggcccct ggggactctc aagtattctt tcggatttaa acgcgacctc
     1081 ccactatgag gggtcttacg tttacaccat ctggtccacg gtagatgaag tgatcggcta
     1141 cggctgtgta gtgtggggac gttatacgcc tcaaatcccg gctcaaaacg gggaaaaacg
     1201 ctataccagc gcaccatatg ggcacttcaa tagtaaggat ttgacgacct actaccagct
     1261 ccggatggtg aaatatcatt caaccacgta aaaccagggc ttagggctag gggctgaaga
     1321 ctcgcaagct cggggcggaa gaaaacgggt tcaataccct cagccttcag cccgagttct
     1381 tcagcccgag ttcttcagcc cgaaaaaata tgccacttgc cacactttca aattcggttt
     1441 cactggccta tgacattcaa ggtgaaggcc ccccactggt tctgatttgc ggtcttggga
     1501 ctggcggctg gatctggtgt aaacaagttc cagagcttgc ccagcacttt caagtcatta
     1561 cctttgatcc gcgtggcgtg ggagaatcaa gccaggttgc ggcgccgttc tcgattgctg
     1621 atctggccga ggatatctcg tttttattgg aacatcttgg tttggagcga gcccatattg
     1681 ttggagcttc gctgggtggg attgttgccc agcaatttgc actgacgttt ccacatctgg
     1741 ttgagcgact ggtgctctcg tgtaccacgt ttggcgggcc aaatcatgtg ctgccgggtg
     1801 gtgaggggct ggcagccttg atgtcagtga gcgggctcaa cggcgaagaa cgtgcccggc
     1861 gcaacatcca gtttgggttt accaaagctt atcttgaaaa taacatggat gatgttgaag
     1921 acatcattcg gatgcggctg gcgtgcaaca ttccagagca cgtctacctg tttcaagtga
     1981 aggctgcttc agaatttaac gccgaacccc acataccagc cattcagt
//
LOCUS       DAOQDO010001239         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54565_length_2028_cov_2.448049, whole genome shotgun sequence.
ACCESSION   DAOQDO010001239 DAOQDO010000000
VERSION     DAOQDO010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54565_length_2028_cov_2.448049"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PKZ53_17625"
     CDS             complement(<1..1294)
                     /locus_tag="PKZ53_17625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNJ42312.1"
                     /translation="MICTIATLPYLGLALTMGSSFIEHHPQGKVYVLLVCEGAASLPV
                     LPATFEVVCAAELGIEDYTGMESRYTAFELCNALKPFWLEYLLLHYGVSKISYFDADI
                     CFFGSIEPVLWKMLDTASIVLTPHFCRLPEDNSDIFQEIAVLRRGVYNGGFIGISNTP
                     DGHAFLKWWKARLGRFGYHRLSESMNCDQLWLDFAPSFGLNVRILNDPGYNVAYWNLH
                     ERHLTTQRPNPAPLKFLVNNHPLVFFHFSGYSPDHPDRITTHWTGHSFSKFPELKPLF
                     DAYQRRLKSLPFDWPQTQSRKPPVRFEPHRPDRPASQPSANRVSVIVTVQNGAGSIQK
                     AIESVLAQTYPDLEIIVVDAGSTDATQVRLEPYIAQIRSFRQPQCRTSAARTLGLSVA
                     TGEFIAFLDAEDSMLRSTRLADQVQVLRRHTDFCLVNSG"
     gene            complement(1324..>2028)
                     /locus_tag="PKZ53_17630"
     CDS             complement(1324..>2028)
                     /locus_tag="PKZ53_17630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42313.1"
                     /translation="LRNPHTLGLAINWNACLQTATGHWIHFLHQDDTLLPGFYDRLRP
                     PIEHNPDIGAAYCRVYGVNASGRITWTSSQDRPEAGIVEDYLERQARWNRVQVVGIVV
                     RRSVYETIGGFNARLCYAPDWDMWKRIGVFSKVWFEPECLACYRHHADSTTNKLRRAG
                     QALTDERRAIEFSRHYLPEHIYRLSTRFALLKNMQWASHSAQHSLKQGDLAGAFNQSL
                     ELSSTALHWLRLNLFG"
BASE COUNT          531 a          490 c          556 g          451 t
ORIGIN      
        1 acccactatt gacgagacaa aagtcggtgt ggcggcgcaa cacctggacc tggtcagcaa
       61 gtcgggttga gcgcaacatt gaatcttcag cgtccagaaa tgcgataaat tcgccagttg
      121 cgacggaaag cccaagggtt cgcgcggctg aagtcctgca ttgaggttgc cggaaactcc
      181 gaatttgagc gatgtaaggc tcaaggcgca cctgtgtcgc atcagtcgac ccagcatcca
      241 cgacgataat ttcaagatcc gggtaggttt gggccaggac actctcgatt gccttttgga
      301 ttgagccggc cccgttttga actgtcacga tcacactgac ccggttggca gaaggctggc
      361 tggctggcct gtctgggcga tgtggttcaa atcggacggg tggtttcctg gattgtgttt
      421 gaggccaatc aaacggaagg cttttgagtc gtcgctggta agcatcaaac aaaggtttca
      481 gttccgggaa ctttgagaat gagtgacctg tccagtgagt cgtaattcga tctgggtggt
      541 ctggtgagta cccgctgaaa tggaaaaaaa cgagtgggtg gttattaaca aggaacttca
      601 gcggggcagg gtttggacgt tgagtcgtga gatggcgttc atggaggttc caataggcaa
      661 cgttataccc tgggtcgttc aaaatccgga cattgaggcc gaaacttgga gcaaagtcga
      721 gccagagttg atcacaattc atactttcac tcaaccgatg atagccaaac cggcccagtc
      781 gtgctttcca ccatttcaaa aacgcatggc catccggagt attggaaata ccgataaatc
      841 caccgttata aaccccgcgt cgcaacacgg caatttcttg aaaaatatca gaattgtcct
      901 cagggaggcg gcagaaatga ggtgtcaaca cgatggacgc cgtatctaac atcttccaga
      961 gaacgggttc aattgatcca aaaaaacaaa tatcagcgtc aaagtagctg attttcgaca
     1021 ctccataatg aaggagcagg tattcgagcc agaagggttt gagtgcatta cagagttcaa
     1081 aagccgtata acgtgactcc atcccggtgt aatcctcaat gcctagctcc gcggcacaca
     1141 ccacttcaaa cgtagctggc aaaaccggaa gactggcggc accctcacaa accagcagta
     1201 catacacctt tccctggggg tgatgttcga taaatgaact tcccatcgtc aaagccaggc
     1261 caaggtatgg aagcgttgca atggtacaaa tcatattggc acagtcagaa gcagcacttc
     1321 aggtcaacca aaaagattga ggcgcaacca gtggagcgcg gtcgaagaga gttcaagcga
     1381 ctggttgaag gccccggcca gatcaccctg ctttaaacta tgttgggctg aatgtgaggc
     1441 ccactgcatg tttttcagga gtgcaaaccg ggtggaaagc cgataaatgt gttcaggaag
     1501 gtagtgtcgc gaaaactcaa tcgcgcgccg ttcgtcggtt aaagcctggc ctgcgcggcg
     1561 caacttattt gtggtggagt ctgcatgatg acggtagcag gccaggcatt caggttcaaa
     1621 ccaaactttg gaaaaaacac cgatccgctt ccacatatcc caatcagggg catagcacaa
     1681 tcgagcgtta aatccaccga tggtttcata gacagaccgt cggaccacga ttccaacgac
     1741 ttgaacccgg ttccatctgg cctggcgttc gagatagtct tcgacaatgc cagcctcggg
     1801 gcggtcttga gatgatgtcc aggtgattct cccggaggcg tttactccat agacccggca
     1861 gtaggcggca ccaatgtctg gattgtgttc aatcggaggg cggagccgat cataaaatcc
     1921 gggtaacagt gtgtcatcct ggtgcagaaa gtgtatccag tggccagttg cggtttgcag
     1981 gcaagcattc cagttaatcg ccaggcccag ggtgtgcggg tttcgcaa
//
LOCUS       DAOQDO010001240         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54622_length_2027_cov_2.329108, whole genome shotgun sequence.
ACCESSION   DAOQDO010001240 DAOQDO010000000
VERSION     DAOQDO010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54622_length_2027_cov_2.329108"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKZ53_17635"
     CDS             complement(<1..205)
                     /locus_tag="PKZ53_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42314.1"
                     /translation="MSWTMLVLLITWLVSVFGVLIGASRYRGGKTNRLKGDAYEALPG
                     ASEGSKRSGKLSKTTTEIRPELSA"
     gene            complement(275..>2027)
                     /locus_tag="PKZ53_17640"
     CDS             complement(275..>2027)
                     /locus_tag="PKZ53_17640"
                     /inference="COORDINATES: protein motif:HMM:NF016796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNJ42315.1"
                     /translation="PPLNLWPAIQNIRRTHDRKINRWMPHITLMYGFLPDHLFAEAAE
                     LIKRELAQIQPFSIRLTKYESFEHRRNATVWLKPEVDPPGALHQLQKRLFQLFPQCSE
                     QNSRPNGFTPHLSVGQVDWLSPTQMKLPLWHPCTFEVHSIALISRVDDMPFEVRYEIE
                     LGTQKADQSAGESTLRDLVLELEPLLTPEEIIHRETILQLVHQACVEVAGEWTTLETV
                     GSARMGVATRSSDLDTVCLIPLELSAGEFLESVASKLSPLAEMTRIVRDAQVSLLKVR
                     IEGVDVDLMPAHRPASLSVTSTIYACYQTEFDPASWQNLVGCFEADDLLKFVTRKVNL
                     TLFQEFLRGVRAWVAQRRIGGNAWGFPGSFSWALMAAWTCQQLEPDMVPDLETLTINF
                     FNQLDEHDWTQPVGLTDGAREFKIRSHRDHLPVITSVAPHQNSARNISRSTAQLIKDE
                     FRRGRECAADHQWLRLFEPVVQAVSGATLNLQYQIEVVVASDFDKVAGWLEGRMFGQI
                     LVLEQRCPGVQVRPWPGVTQTNLTGTVTLDLCQSSPADRPAIEEITHTLVQQFQTWAD
                     RPDGAKLDVKVNDTNLG"
BASE COUNT          501 a          536 c          516 g          474 t
ORIGIN      
        1 aggcagacaa ttccgggcga atttcggtcg ttgtttttga cagtttccct gatcgttttg
       61 atccttcgga agctccaggc agcgcctcgt aggcatcacc tttcaggcga ttggttttcc
      121 cacctcgata gcgtgaagca ccgatgagca cgccaaatac cgacaccaac cacgtaatga
      181 gcaaaaccag cattgtccag gacatatggg gaattatgaa tcaggctaaa gtattgaaaa
      241 ttttgcattt ccctcaatct ctaaaccgac actctcagcc caaattggta tcatttacct
      301 ttacgtcgag tttggcgcca tccgggcggt ccgcccaggt ttgaaactgc tgaaccagcg
      361 tgtgggtaat ttcttcgatg gcgggccgat cagccggact tgattggcac agatcgagcg
      421 tcacggttcc ggtgagattg gtttgggtaa ctcctggcca gggacgaacc tgcacaccag
      481 ggcagcgttg ttcgagcact aaaatttggc cgaacatccg accttcaagc cagcccgcaa
      541 ctttgtcaaa gtctgaagct accacgactt caatctggta ttgaaggttc aaagttgcac
      601 ctgaaacagc ttgaaccaca ggttcaaaca gccttaacca ctgatggtcg gcagcgcatt
      661 ccctgccgcg tcggaattcg tccttgataa gttgtgcggt tgaacgcgaa atatttcgag
      721 cactgttttg atgtggcgcc accgatgtaa tcaccggcaa atggtcgcga tgcgaacgaa
      781 ttttgaactc cctggcacca tcagtcaatc cgactggctg cgtccagtcg tgctcatcaa
      841 gctggttgaa aaaattgatc gtcagcgttt ccaggtctgg caccatgtct ggttcgagtt
      901 gctgacaggt ccaggccgcc attaacgccc aggaaaagct gcctggaaat ccccaggcat
      961 tgccgccgat gcgtcgctgt gccacccagg cccgaacacc acgcagaaac tcctgaaaca
     1021 aggtcaggtt cacttttcgg gtgacaaact tcaataaatc atcggcttca aaacagccaa
     1081 ccagattttg ccagcttgcg ggatcaaact cagtttgata gcaggcataa atggtggacg
     1141 tcactgaaag cgaagcggga cgatgtgccg gcatgaggtc aacgtcaacg ccttcaatcc
     1201 gaaccttcag cagcgagacc tgggcatccc gtacgattcg cgtcatttcc gccagtggtg
     1261 aaagtttgct tgccactgat tccagaaatt caccggctga taattccagc gggatgaggc
     1321 acacggtatc aagatcactc gaccgggtgg caacgcccat tcgggcggaa ccaaccgttt
     1381 ccagggtcgt ccattccccg gcgacttcga cacatgcctg atgaaccagt tggaggatgg
     1441 tttctcgatg aatgatttct tccggagtca acagtggttc tagctccaaa acaaggtctc
     1501 tcagggttga ctcacccgct gattgatccg ccttttgagt tccaagttca atttcataac
     1561 gcacttcaaa cggcatgtca tcaacccggc tgataagcgc gattgaatga acttcaaaag
     1621 tacagggatg ccacagaggc aatttcatct gagtcgggct tagccagtca acctgaccga
     1681 cgctcagatg aggcgtaaac ccgttgggac ggctattttg ctcagaacat tgaggaaaca
     1741 actgaaacag tcgcttctga agctgatgca aagcccctgg cgggtcaact tcgggtttga
     1801 gccagaccgt ggcatttcgg cgatgctcaa aagattcgta cttcgtcagc cgaatcgaaa
     1861 acggctgaat ctgtgccagt tcccgcttga taagttcagc cgcttcggca aagaggtgat
     1921 caggtaaaaa cccatacatc agcgtgatat ggggcatcca gcgattgatt ttgcggtcgt
     1981 gcgttcgccg aatgttctga atcgcaggcc agagattgag tggcggg
//
LOCUS       DAOQDO010001241         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54657_length_2026_cov_3.478437, whole genome shotgun sequence.
ACCESSION   DAOQDO010001241 DAOQDO010000000
VERSION     DAOQDO010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54657_length_2026_cov_3.478437"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1157)
                     /locus_tag="PKZ53_17645"
     CDS             complement(33..1157)
                     /locus_tag="PKZ53_17645"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNJ42316.1"
                     /translation="MKAYFLVCLVFLGSLGIKADFTQSPNAQTPPAEVQPGTQEEKVY
                     TSLAEALKEPLKVKVLALHNLDSIPADIAKLENLTDLTIVRFSKSKLPSVLWTLSKLK
                     SLRILECPIIELSPEIGKLRNLQALHLMGSQVKSLPTEIGSLKSLETLDLHANQLNSL
                     PDSICLLGLLSDLGVSDNQLKSLPNQLEKLTLLEAMDLSGNQLELLPPEITRLQKLEL
                     LDVHHNQLKDLPIGLNKMQSLYTLHAGFNLYSKLPFDFHSFPKLKMVILDLQRVPNRQ
                     IEQLTNTSSQDLILVSTGEKFISGRINIGKVDQPNPAKRSTRVSLLNATERGSRTQDK
                     AYQTIYRLNLFQTNDQITRPVVTRSIKPRYSNRASGLRLN"
     gene            complement(1198..1968)
                     /locus_tag="PKZ53_17650"
     CDS             complement(1198..1968)
                     /locus_tag="PKZ53_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ42317.1"
                     /translation="MRRVLFQICAIACLLVVTLAMVGDRSRAQQTPALRVLFIGNSLT
                     YVNNLPAVLEAMARESGQVPLVYQTIAFPDFSLEDHWKQGDARKTISTGKWDVVVMQQ
                     GPSALPESRVLLLEYVKKFAQEIRNSGAKPALYMVWPAKERSFDFDRVSESYRLAAQE
                     VDGLLFPVGDAWRTAWKHDASLPLYSADKFHPSKAGTYLAAAVMYEQLYRRSSVGLPA
                     QLKISDPEVKKIKLPRKQAEALQMAATETNQGQTPTGS"
BASE COUNT          531 a          433 c          489 g          573 t
ORIGIN      
        1 agtcgctatt ttgctttctc attggtgtta agtcaattca gccgaagacc actagcccgg
       61 tttgaatacc tcggttttat tgaccgagta actacgggtc ttgtgatttg gtcatttgtt
      121 tgaaaaagat ttaatcggta gatggtttga tacgctttat cttgagttct tgaacccctt
      181 tcagttgcat ttagtaacga gacacgagtg cttctctttg ctggatttgg ctggtcaact
      241 ttcccaatgt taattcgtcc actaataaac ttttcgccag ttgaaacaag aattaaatct
      301 tgagaggaag tatttgttaa ttgttcaatt tgtcggtttg ggactctctg aagatcaagg
      361 attaccattt tcagtttagg aaaagaatga aagtcaaatg gaagctttga atacagatta
      421 aatcccgcat gcagagtgta aagtgactgc atcttattca acccaattgg gagatctttc
      481 agctgattat gatggacatc taacaattcc aatttttgga gcctggtaat ttctggtggg
      541 agcaactcca gttgattccc gctcaaatcc attgcctcta gcaaagtaag tttttcaagc
      601 tgatttggca atgatttcaa ctggttatca ctgactccta aatccgaaag taatcctaat
      661 aagcagatac tatcaggtaa tgaattcagt tgattggcgt gaaggtcaag agtttcaagc
      721 gatttcaaag atccgatttc agttggcaag cttttcactt gagagcccat caagtgcaaa
      781 gcctggagat ttctaagctt tccaatttca ggcgacagtt cgatgattgg acattctaaa
      841 attctgagtg atttgagttt agagagggtc cataaaacag aggggagttt agattttgag
      901 aaccttacaa tggtcaaatc tgtcagattt tcaagtttgg caatgtctgc aggaatggaa
      961 tcaagattgt gtaaggcaag aactttaacc ttgagtggtt ctttcaatgc ttccgcgaga
     1021 gaggtgtaca ctttttcttc ctgagtccca ggttgaacct ctgctggtgg agtttgagca
     1081 ttcggagatt gagtaaaatc agctttgatg ccaagactcc ccagaaatac cagacaaacc
     1141 aggaaatacg ccttcattga aaaacctcat ttttcaactg gttgttaact tgtcatttca
     1201 cgagccagtc ggagtttgcc cctgattcgt ctcagttgca gccatctgaa gcgcttcggc
     1261 ttgcttgcgg ggcaatttga tctttttgac ttcgggatcc gaaatcttga gttgggcagg
     1321 aaggccgacg gagcttcggc gatacagttg ctcatacatc acggcagcgg ccaggtaggt
     1381 gccagctttt gaagggtgaa atttgtctgc gctgtatagc ggcaaactgg catcgtgttt
     1441 ccaggcggtc cgccaggcgt ctccgacggg gaagagcagg ccgtcaactt cctgggctgc
     1501 cagtcggtag gactcgctga cccggtcaaa atcaaacgaa cgctctttgg ccggccagac
     1561 catatacagt gccggtttgg cgccgctgtt gcggatttct tgagcaaact ttttcacata
     1621 ttcgagcagc aagacgcggc tttccggcag ggctgacgga ccttgttgca tcacaacgac
     1681 atcccatttc ccggtggaaa ttgttttgcg ggcatctcct tgtttccagt ggtcttccag
     1741 gctgaaatca gggaacgcaa tggtttgata caccagcgga acctggccag attcgcgggc
     1801 catggcttcc agcaccgcag gcaggttgtt gacgtaggtc aaactgttcc cgataaacag
     1861 cacccggagc gccggcgttt gttgggctcg actccgatct ccaaccattg ccagcgtaac
     1921 caccagcaaa caggcaattg cgcaaatctg gaacaataca cgtctcataa gccaatgaag
     1981 aatgaagaat gaagaatgaa gaatgaagaa tgaagaatga agaatg
//
LOCUS       DAOQDO010001242         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54678_length_2026_cov_2.368848, whole genome shotgun sequence.
ACCESSION   DAOQDO010001242 DAOQDO010000000
VERSION     DAOQDO010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54678_length_2026_cov_2.368848"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="PKZ53_17655"
     CDS             <1..1866
                     /locus_tag="PKZ53_17655"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNJ42318.1"
                     /translation="QAMRTDETRSTKTVDTLPVQAQPRQGGGYHFFKWLTLALVVGLV
                     AGSGLWGARLWLQARQKTSLGDVKAVQTTISSGLDVYPTFSPDGRSMVYCSDKSGGFE
                     LYIKQLVAGGRELQLTTDGEQNVQPAWSPDGEWIAYHSKNRSGIWVISVLGGSPKQLS
                     EFGSRPIWSPDGKLLAFQSDGLTDLSGNAAPAQPPSTIWTVPARGGNPQAVTQVGNPA
                     GGHGTPSWSADGKWIAFATYDRRTSAIWAISTESKELVRIVATQRYIYDPIFAPDGKG
                     IFYSAVLEDGNYGMWKIDVSPTSCEPDGEPYQLANLGIGTPRHLTISKDGKKIAYSAL
                     SMTSNLWSFELDPRTQTSKTKPVPLMLETGRSTRPVFSPDGSKIAFEKWRQGTNPDIW
                     MVDADGNHQTQLTTDPAVETIPSWSPDSSKIYFRSNREGQQAFWSIVPESRKETLLQK
                     FEKDTDFPRLSPDGTMVAFNSKRDGTTTNVWLVPIEGGQPKQITFDQESLGFPCWSPD
                     GKWVALQLKRGEDTQLVVMPSQGGEITQLTTDRGQHWPHSWSPDGDKIAFAGFRQGYW
                     NLWIISRTTREQKQLTGYTKLNAYVRYPAWSPKGNQMVFEYAETVGNVWMIEIQK"
BASE COUNT          504 a          526 c          538 g          458 t
ORIGIN      
        1 caagcgatgc ggaccgatga aacccgcagc accaaaacgg ttgatacact tccggttcag
       61 gctcaacctc gccagggtgg cggataccac tttttcaagt ggctgaccct ggcactggtg
      121 gttggactgg tggctggaag cgggctctgg ggagccaggc tctggcttca agcccggcaa
      181 aaaacaagcc tgggagatgt aaaagccgtc caaaccacga tttcctcggg gcttgacgtg
      241 tatccgacct tttcacctga cggacggtca atggtgtatt gctcagataa aagcggtggc
      301 ttcgaactct atatcaaaca actggtggct ggaggccgtg aactccagtt gaccaccgac
      361 ggcgagcaga atgtgcagcc cgcctggtca cccgatggcg aatggattgc ctaccacagc
      421 aaaaaccgga gtgggatttg ggttatttcc gtgctgggtg ggtcacccaa acagcttagt
      481 gagtttggtt cacgaccaat ctggtctcct gacgggaaac tcctggcatt tcaatcggat
      541 gggttgactg atttgagtgg caatgccgca ccggctcagc ctccgtcaac tatttggacc
      601 gtgcccgctc ggggagggaa cccgcaagcc gtgacccagg tgggcaatcc agccggaggt
      661 cacgggacgc catcctggtc agccgatggg aaatggattg catttgctac ctatgaccgc
      721 cgaacttcag ctatttgggc gatttcgact gaatccaaag aactggtgcg gattgtggcg
      781 acgcaacgct atatttacga cccgattttt gcacctgatg gaaaggggat tttctattca
      841 gccgttttgg aagatggaaa ctacggcatg tggaaaattg atgtttcgcc aacgagttgt
      901 gaacccgatg gcgagccata tcaactggcc aatctgggca tcgggacgcc tcgccacctg
      961 acgatctcaa aagatggaaa aaagattgcc tacagcgcac tttcgatgac cagcaatcta
     1021 tggtcgtttg aacttgaccc cagaacccag acttcaaaaa ccaaacctgt tcccttaatg
     1081 cttgaaaccg ggcggagtac ccgtccagtc ttttctcctg atggtagcaa gattgccttt
     1141 gaaaaatggc ggcaagggac caatcctgac atctggatgg tggatgccga cggaaatcat
     1201 caaacgcaac tcaccaccga cccggcggtt gagaccattc caagctggtc gccagacagt
     1261 tcgaagatct atttccgatc caaccgcgaa ggccagcagg cattctggtc aattgtgccg
     1321 gaaagccgca aggaaaccct gctccagaag tttgaaaagg acaccgattt cccacggctt
     1381 tcccctgatg gaacaatggt ggcgtttaat tccaagcgcg atgggaccac caccaacgtc
     1441 tggctggtgc cgattgaagg cggccagcca aaacagatta cttttgatca ggaatcgctt
     1501 ggattcccct gctggtcacc agatggaaaa tgggtcgccc tccagcttaa acgcggcgaa
     1561 gatactcagc tcgtcgttat gcccagccag ggcggggaaa tcacccagct taccactgac
     1621 cgaggccagc actggccgca tagctggtca cccgacggtg acaaaattgc ctttgctgga
     1681 ttccggcaag gctactggaa tctctggatc atttcgcgaa ccactcgcga acaaaagcag
     1741 ctcaccggct ataccaaact caatgcctat gtccggtatc cggcctggtc gccaaaagga
     1801 aatcagatgg tgtttgaata tgccgaaacc gtgggcaatg tatggatgat cgaaatacag
     1861 aaatgaagaa ttgagaatat tttttgggag caccgatatc ctgccggcag gatgtcggca
     1921 cttccagcca ggtttctttt tctggttaga ttcaaattga atttcgaatt taaccgcgaa
     1981 tttaatttta aattgatttc tagtgatggt ttcggtacct gccttt
//
LOCUS       DAOQDO010001243         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54709_length_2025_cov_4.010152, whole genome shotgun sequence.
ACCESSION   DAOQDO010001243 DAOQDO010000000
VERSION     DAOQDO010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54709_length_2025_cov_4.010152"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="PKZ53_17660"
     CDS             <1..1465
                     /locus_tag="PKZ53_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42319.1"
                     /translation="LIGLLFLSIIYAVYAWRIHTLSARQQQKLMHLKQLQNQRLITMR
                     QLLGSIQVINSQLEITAVLQNIAEESARLVDGIPGGIGLVQGDRVVFQRQWLTDGWQA
                     SNVWFQFGEGTPGKVARSAKAIIVNNPEDEPISFAPEQARKSALQGWMDVPILTRSGT
                     VTAVLNIRHRPGRPPFTSEDLNLIELLASQAAVAIENASLYGEVEEKNLMIAESFHEL
                     ERLYQQEQEVSRTLQELNQMKTNFIVVTSHEMRTPLTVIRGYTEALLSEFFGQLNPVQ
                     HQSLSVTQRTVDRMIDNFNNILEMLKINEGKFTLRPEPTSLKELIHEVREELGPFIEK
                     RKQTLTVIGTDFVAGVDRQKFGLVLLNVVQNAIKFTPDDGTIQIALEEEEKSIHIQVR
                     DTGIGIEANELDRIFDRFYTTSDPSTHRSGHYEFSTRGSGLGLAIAKSYVIAHGGRIW
                     ASSDGKDKGCTLHLVIPARPQLNPVPSASSRCRAEEG"
BASE COUNT          524 a          498 c          497 g          506 t
ORIGIN      
        1 cctgattggg cttttgttcc tttccattat ttatgccgtc tatgcctggc gcattcatac
       61 attgagtgca cgccagcaac aaaaactgat gcacctcaag cagttacaga accagcgcct
      121 gattaccatg cggcagttgc tggggagtat tcaggtgatt aactcgcaac tggaaattac
      181 ggctgtgctg caaaacattg ccgaagaaag cgcccggctg gtggatggaa tccctggcgg
      241 tatcgggctg gttcagggtg atcgggtggt ctttcaacgg caatggctca ctgatggatg
      301 gcaggcgagc aacgtctggt tccagtttgg cgaaggcacg ccaggaaaag tcgccaggtc
      361 agccaaagcg atcattgtca acaaccccga ggatgagccc atcagttttg ccccggaaca
      421 ggctcgaaaa agtgcactcc agggctggat ggatgtacca atcctgaccc ggtcaggcac
      481 ggtaaccgca gtgctcaaca tccgtcatcg ccctgggcga cctccgttta ccagtgaaga
      541 cctcaacctc atcgagttac tggcgagtca ggccgccgtg gcgattgaaa acgccagttt
      601 gtatggtgaa gtggaagaaa agaatttgat gattgccgaa tcatttcacg aactggaacg
      661 gctctatcaa caggaacagg aagtgtcgcg caccctgcag gaactcaacc agatgaagac
      721 caatttcatt gtggtcactt cgcacgagat gcgaaccccg ctgacggtaa ttcgtgggta
      781 taccgaggcg ctcttgagcg aatttttcgg ccaattaaac ccggtccaac accagtcact
      841 ctccgtgacc cagcgcaccg ttgaccggat gattgataac tttaataaca tcctggaaat
      901 gctcaagatc aatgaaggga agttcaccct ccgtccagaa ccaacgtctc taaaagagtt
      961 gatccacgaa gtccgggaag aactgggccc ctttatcgag aagcggaagc aaacattgac
     1021 ggtcatcgga actgattttg ttgccggggt ggaccgtcag aaatttggcc tggtgctgct
     1081 caatgtggtg caaaacgcca tcaagttcac accagacgat ggaaccattc aaattgcgct
     1141 ggaagaagaa gaaaaatcaa tccatattca ggtccgagat accgggattg gtattgaagc
     1201 caatgagtta gatcgaattt tcgaccggtt ttacaccacc tctgacccct ccacacaccg
     1261 ttccggacac tatgaatttt caactcgcgg atcaggattg ggcctggcaa ttgccaaaag
     1321 ttacgttatc gcgcacggcg gacgaatttg ggccagttct gacggaaaag ataaaggatg
     1381 caccctccac ctggttattc ccgccaggcc acaattgaac ccagttccat cggcatccag
     1441 ccggtgccgg gcagaagagg ggtgatctca ccacatcctt tcgcccacag atgttcagaa
     1501 tcagaaacca cggaatccac caaacacacc gaagataaaa caatattttt cgacgggttc
     1561 tgatctatta cctggatgct ggccaacgat attgccctct tctttgcctg gactgaattt
     1621 gctcagtcca ggccattgtt tttctgggag ggcgttgaaa ataagataaa tgaagcaatt
     1681 tgtgtcttaa tgagtgacac ttttaggggt atacgacaaa aaatgagtca gttttttgga
     1741 agatatacgt taactgaaga tattgctatc tcctttattt acagttggtt cactgggttt
     1801 tgtgcggctg gcacgtgagt tgctgatgct ttgcctttcc tgaccccgca cctcgtctct
     1861 ggttgttcct tacattgacg ttccctggca tagatcactt gttcagagtg aaaaggagtt
     1921 tcttccagac atgagttatc tcaattttca gaatggaaaa agcaaagtct atttcagttg
     1981 tgtattgttc ttttttctta cacttgttgc cgtcccacat tttag
//
LOCUS       DAOQDO010001244         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54715_length_2025_cov_3.532995, whole genome shotgun sequence.
ACCESSION   DAOQDO010001244 DAOQDO010000000
VERSION     DAOQDO010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54715_length_2025_cov_3.532995"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKZ53_17665"
     CDS             <1..901
                     /locus_tag="PKZ53_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42320.1"
                     /translation="YGVDYAQLSQAQDTTIQPYIGLRWALTPHTQLFGAVSHEGDLSF
                     DATPHLEDRSTPFQSIAEPQLAVTTSTPDDLPRMQLDRTRRYEAGLAHQLTSRSTIET
                     TLFLDTVSGHGIGLALLEPNSVLTDRVRQTPLIIGQNGVQHGVRLWYRRRLTPTVSVA
                     AGYAAGYAQKLALTSATEIDPRATSKIEFQPSRFQVLTARIDAHILQTGATITAVVRK
                     TLGNPVFAVDPFQGRLPALDRGISIFVSQPIPLPQLIPGRWEILIEGRNLFDQTEDLS
                     AEGMTIQWLSTRRTLRGGLGLRF"
     gene            962..>2025
                     /locus_tag="PKZ53_17670"
     CDS             962..>2025
                     /locus_tag="PKZ53_17670"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ42321.1"
                     /translation="MKPAVNSKTLFVGISTVICLLLASLNLFERLAQKPLADDGVIWQ
                     SAAQGLRVQYVRPGSAADRAGLVPGDILRALDFDGQPIAIDADWQVQYYLDEKIGIGG
                     KVTYLVECFNPLGLSKGLWAAELYDIGTQPSRFTIDLYLGFIGLIYLLIGLFVFHKQD
                     HTPQTLHFYFICLLSFLYLVLSPSGDFDWFDKLVSLIDNAALTLLCPVFLHFCSQFPR
                     PSPWLEKHPWATRILYVPALFLIGLEITHLTLGKENLNLLEIRPWLNTAEIAHFTLFF
                     ISSASVVIVTFTQTRASMLRQQIKWIVWGLSLGIFPWTIFYAYPYLAGIGITPLMTAL
                     AVGPTVFIPLAFGYSIVRYR"
BASE COUNT          393 a          658 c          458 g          516 t
ORIGIN      
        1 ttatggtgtt gactatgccc aactcagcca ggcccaggat acaaccatcc agccctatat
       61 cggcctgcga tgggcactca cccctcacac ccagttgttc ggcgccgtgt ctcacgaagg
      121 tgatctttca tttgatgcca caccccacct cgaagaccgg tccactccgt ttcaaagcat
      181 cgccgagccc caactggccg tgacaacgtc aactcccgac gatcttcccc ggatgcaact
      241 tgaccggacc cgacgctacg aagctggcct cgctcaccag cttacctccc gatcaaccat
      301 cgagacgacc ctgttcctcg acaccgtctc tggtcatggc atcggtcttg ccctcctgga
      361 gccaaattcc gtcctgacag atcgcgtccg ccagaccccg ctcattatcg ggcaaaacgg
      421 ggttcagcac ggcgttcgcc tgtggtaccg tcgccgactc accccaaccg tctcagtcgc
      481 ggctggctat gcggctggct atgctcaaaa actggcgctg acctcagcca ccgagattga
      541 tccccgcgcc acttccaaaa ttgagttcca accctcacgg tttcaagttc tcaccgcccg
      601 gattgatgct catatcctgc aaactggcgc caccatcacc gctgtggtgc gcaaaacact
      661 gggcaatccc gtttttgccg tggatccatt tcaaggccga ctcccggccc tggatcgtgg
      721 aatcagtatt tttgtctcgc aaccgattcc actcccccaa ttaatcccag gtcgctggga
      781 aattctgatt gaaggccgga acctgtttga ccagaccgaa gacctgtcag ccgaaggcat
      841 gaccattcaa tggctctcaa ctcgccgaac gctccgtggc ggtcttggct tgcgcttcta
      901 atcatcgcaa ccgggagcat ctcgctcccg ctcatcccgt ctttttcacg ttgtgccgct
      961 tatgaaacct gcagtgaatt ccaaaacact ttttgttgga atttcgactg tcatttgcct
     1021 gcttctggca tcgctgaacc tctttgagcg actggcccaa aaaccactcg ccgatgatgg
     1081 cgtcatctgg caatcggctg cccagggact gcgggttcag tatgtccggc cagggagtgc
     1141 ggcagatcgg gccgggcttg tcccaggcga tatcctgcgg gcacttgatt ttgacggcca
     1201 gccgattgcg attgatgcgg actggcaggt tcaatactac ctcgatgaaa agatcggaat
     1261 tggcggcaaa gtgacctatc tggttgaatg cttcaacccg cttggcctga gcaaaggatt
     1321 gtgggcggct gaactctatg atattggcac tcagccctcc cggtttacga ttgacctgta
     1381 cctgggtttt atcggactca tctatctcct catcgggctg tttgtttttc acaaacagga
     1441 tcacactcca caaacccttc acttctattt tatctgcctc ctgtcattcc tctacctggt
     1501 cctgagccca agcggcgact tcgactggtt tgataaactg gtctcactca tcgataatgc
     1561 cgccctcacc ctgctctgtc ccgtctttct tcatttttgc tctcagtttc cacgtcccag
     1621 cccctggctt gaaaaacacc cctgggccac tcgcatcctc tatgtcccgg ccctcttttt
     1681 gatcggtctg gaaatcaccc acctcaccct cggcaaagag aacctgaatt tgttggagat
     1741 ccgcccctgg ctcaacaccg ccgaaatcgc tcattttacc ctgttcttta tctcaagcgc
     1801 ctcagtcgta attgtcacct ttacccaaac ccgcgccagt atgctccgtc agcaaattaa
     1861 gtggattgtg tgggggttga gcctgggtat tttcccctgg accattttct atgcctatcc
     1921 ctatctcgct ggcatcggca ttaccccgct gatgaccgcc cttgccgtcg gccctacggt
     1981 ctttattcca ctcgcctttg ggtattcgat tgttcgctat cggtt
//
LOCUS       DAOQDO010001245         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54718_length_2025_cov_3.227411, whole genome shotgun sequence.
ACCESSION   DAOQDO010001245 DAOQDO010000000
VERSION     DAOQDO010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54718_length_2025_cov_3.227411"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..816)
                     /locus_tag="PKZ53_17675"
     CDS             complement(85..816)
                     /locus_tag="PKZ53_17675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ42322.1"
                     /translation="MTDYYKPDLAFIHDVGFANFALTAAPGILQCLHQNQIHRGLIVD
                     LGCGSGIWAEQLHRAGYQVLGVDISPAMIDLARNRVPGAQFQVASLFQAEIPPCVAVT
                     SMGECINYLFDPENTRGQISQLFRRIYQALHPGGLLIFDIAEPGQCPPNITIKHFTEG
                     PDWLVLVEKTEDPETAILTRRIITFRQAGDLYRRDEEIHSQQLYQANDLVDELRQIGF
                     EVYTSPQYGDQPLPPARMALIACKR"
     gene            complement(855..1385)
                     /locus_tag="PKZ53_17680"
     CDS             complement(855..1385)
                     /locus_tag="PKZ53_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42323.1"
                     /translation="MTTHISQIGAPPTQRTSQIMFLAGISIAMVVSVLFHVTPALDAA
                     TTLYVYHGLRGIGVEPVVMYGGLTVAYGLMTGLLLTGFLVLFKSLISKELLLVAGLIS
                     GLGLGYCFSTWNTAHIWFWCGNTTEQEKQQIKRDHVQHFSITGALTGLNISLLIIILE
                     LDRQKKFQQSCGANST"
     gene            1602..>2025
                     /locus_tag="PKZ53_17685"
     CDS             1602..>2025
                     /locus_tag="PKZ53_17685"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ42324.1"
                     /translation="MSTYATTRPQPDEHTPYHTMYLSKIPEGDILELLIKHGEDIHHQ
                     LSQIAEEQSAFRYAEDKWSLKQVLGHLIDTERIFQYRLLRVARQDQTPIEGFEQDDYV
                     ASGEFDARSWASLLEEYTTVRAATVSLIKGLPNDAWLRR"
BASE COUNT          556 a          471 c          502 g          496 t
ORIGIN      
        1 aacaaaatga ttggttatga gaaaacagcc ggatgttcaa tcaaacaatg gtacttaggc
       61 cactgattac aacctccaat ggtatcaacg tttgcaggca atgagtgcca ttcgggccgg
      121 cggaagtggt tgatctccgt attgaggtga agtgtaaacc tcaaatccaa tctgacgcaa
      181 ttcatcaacc aggtcattgg cctgataaag ctgttgtgag tgaatttctt catcccgtcg
      241 gtagaggtca ccagcctgtc gaaacgtgat gatgcggcga gtcaggattg ctgtttcagg
      301 gtcttcggtt ttttcaacca gcaccagcca gtcagggcct tcggtaaaat gtttgatagt
      361 tatgttgggc gggcactgtc ctggttcagc aatgtcaaaa atcaggagac ctcctggatg
      421 caatgcctga taaattcgtc gaaaaagctg actgatttga ccgcgagtgt tttcaggatc
      481 aaacaagtag ttgatacatt cgcccattga cgttaccgca acacagggtg ggatttcagc
      541 ttgaaacaga gaggcaacct gaaattgcgc ccctggcaca cggtttctcg ccagatcaat
      601 catggctgga gagatatcaa ctccaaggac ttgatacccg gcgcggtgta attgttcagc
      661 ccagattcca cttccacagc caagatcaac gatcaatcca cggtgaatct ggttttggtg
      721 caggcattgt aaaattccag gtgctgccgt gagggcaaaa ttggcaaagc cgacatcgtg
      781 gataaaggcc agatcaggtt tgtaatagtc agtcatagca aagaatgatt gcaaacacag
      841 attaccattc agtgttaggt ggaattggcg ccgcaagatt gttggaattt cttctgccgg
      901 tcaagttcaa gtatgatgat cagcagactg atgttcaatc cggtcaaggc gccagttatg
      961 gaaaaatgtt gaacgtgatc gcgtttgatt tgttgttttt cctgttctgt ggtgttcccg
     1021 caccagaacc agatatgcgc agtattccag gtgctaaaac agtatcctaa cccaagcccg
     1081 gagatgagac ctgccacgag cagcaattcc ttggaaatca gcgatttgaa cagcacaaga
     1141 aacccggtga gcagcagacc tgtcatcagt ccgtacgcca cagtcaggcc accgtacata
     1201 acaacgggct caacgccgat gcctcgcaat ccgtgataca catacagcgt ggtggcagca
     1261 tccagagccg gtgtcacatg aaataataca ctgaccacca tggctatcga gatacctgcc
     1321 aggaacatga tttgagaggt tctctgagtc ggtggcgcac caatttgtga aatatgggtt
     1381 gtcattttcg gtggctcctg tatacaaaac tggatttctg agtttgacac cagattcaga
     1441 aatccagttc caccaacatg acgagaccca aagcagtcgg caaaagaggt tggaacacaa
     1501 tattgctctg tgatagcaag cgactgtcaa cgatgaagcc ttttctgaga aattctatct
     1561 attcaggtgc ccgtcaccac cctttggaga tctcgaactt tatgtcaacc tatgccacaa
     1621 cacgccccca accagatgaa cacacgcctt atcacacaat gtatctgagc aagattccag
     1681 aaggagatat tttggaactt ctgatcaagc atggtgagga tatccaccac cagctcagtc
     1741 agatagcaga agaacagtca gcttttcgtt atgcggaaga taagtggagt ttaaaacagg
     1801 tgcttggcca cctgattgat accgagcgga tttttcaata tcgcctgctg cgggttgccc
     1861 gccaggatca aacgccaatc gaagggtttg agcaggatga ctatgtcgcc agtggcgaat
     1921 ttgatgcccg ttcatgggca agtttgcttg aagaatacac cacggttcgt gcggcaaccg
     1981 ttagtttaat taaggggtta ccgaacgatg cctggctccg gcggg
//
LOCUS       DAOQDO010001246         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54871_length_2022_cov_3.536858, whole genome shotgun sequence.
ACCESSION   DAOQDO010001246 DAOQDO010000000
VERSION     DAOQDO010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54871_length_2022_cov_3.536858"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKZ53_17690"
     CDS             complement(<1..1022)
                     /locus_tag="PKZ53_17690"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HNJ42325.1"
                     /translation="MVHRKNTLLVVLCLALAGWVVLVSAFHQESVSQAGSQAGRLSTT
                     SLIDFFPQISKPALSGIKPKNPKKVVATSEATSSFVNEVNLSRYVGFIKELADFKSRY
                     STSPGCDAAANYLVKHFSGLGFKTELQPFSIGPRRASNVVATLPGTADKDAKGAFLVF
                     GAHYDSISRDSDPLVTAPGAEDNASGTAALMELAQLFHDHPPRTTVVLVAFAGEEEGL
                     IGSTAYVDSLRTGGSDKRMQAALIMDMIGYTKDADLDILFETSSKFESLVALPTSLAS
                     QFTKLRTETSLRPFGSDHVPFLNENLPAFLVIENDWDAYPAYHRSSDVPANISNDMAG
                     ESMKLVA"
     gene            1277..>2022
                     /locus_tag="PKZ53_17695"
     CDS             1277..>2022
                     /locus_tag="PKZ53_17695"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNJ42326.1"
                     /translation="MNHPHLSAEWIFFDIGGTLLEIYRPLAELVHDYLAREGYQVPVE
                     PLYQAVRTTVASLPTRHPSYTSLEANRDWWMHLYEGTLKLGIGTQEVPQLVLAIVRDA
                     LWEQHRRGDNLRVYADAEGVVETLSERGFRLGVISNWDDTLPPILDRFGLLTAFEILI
                     VSSDLGLEKPDPTIFQHALERAGIDPAASIHIGDDWQCDVVGAAQTGIQPIWIDRGMR
                     KVVVQSTLPDQTELPIKPVTITTLSTVTSFL"
BASE COUNT          513 a          522 c          498 g          489 t
ORIGIN      
        1 gcaaccagtt tcatggattc acccgccatg tcatttgaaa tattggctgg gacatctgac
       61 gaccggtgat aagccgggta cgcatcccag tcattttcaa ttaccagaaa cgccggcaaa
      121 ttttcattca aaaacgggac gtggtcgctt ccaaaaggcc gcaacgacgt ttcggtccga
      181 agtttggtaa attgcgaggc gagactggtt ggcagggcaa ccaatgactc aaatttggaa
      241 ctggtttcaa acaaaatgtc gaggtcagca tctttggtat agccgatcat gtccataatc
      301 agtgccgcct gcattcgttt gtcactgcca cctgtccgaa gtgaatcaac ataggcggta
      361 cttccaatca aaccttcttc ttcaccggca aaagccacca acacaaccgt ggtccggggt
      421 ggatgatcgt gaaagagttg cgccagttcc atcaaggctg cggtgccgct ggcattatct
      481 tcagctcctg gggcagtgac gagtgggtca gaatcccgcg agatcgagtc ataatgcgcc
      541 ccaaacacca gaaacgcgcc tttggcgtct ttatcagcgg ttccaggcaa cgtcgcaacc
      601 acattggatg cccggcgggg accaattgaa aatggctgga gttctgtttt gaatcccaga
      661 ccggaaaagt gcttcacaag gtagttcgct gcagcatcac acccaggaga tgtgctataa
      721 cgggacttaa aatcagccaa ttccttgata aaacctacat accggctgag gttgacttca
      781 ttcacaaatg acgatgtcgc ttctgaggtg gcaacaactt ttttgggatt cttgggtttt
      841 attccggaga gtgccggttt tgaaatttgc gggaaaaaat caatcaagga agttgttgag
      901 agccgaccag cctgactccc agcctgactg accgactcct gatgaaacgc tgaaaccaga
      961 accacccaac cagccagcgc cagacacaac acgaccagca aggtattttt gcgatgaacc
     1021 ataacctttg aagtactcct gaaagaaaag cattacgcgg gaatgacttg cagcatacca
     1081 tattggggaa tcagcgagta gcgcataaaa gtgagtcgcg cgtaatgaag aggtcagccc
     1141 atcgctctct gagttcacgg cttccaactt tgagaattcc tgagttacca cagccacatc
     1201 tcttcgcaag ttcaccctaa agaagttttc tgactattcg caactcactc acattcccga
     1261 ctcgctcctg tgatctatga accatccaca cctttcagca gaatggattt ttttcgatat
     1321 tggcggcaca ttgctggaaa tttaccgccc tctggctgaa ctcgttcacg actatttagc
     1381 cagggaaggc tatcaggtac cggttgaacc actgtatcag gcagtgcgca ccacggttgc
     1441 atcgcttccg acgcggcacc cgagctacac cagtctcgaa gccaatcgtg actggtggat
     1501 gcacctgtat gaaggaacac tcaagctggg gattggtaca caggaggttc ctcaactggt
     1561 tttagctatt gtccgcgatg ccctctggga acaacatcgc cgtggggata atttgcgagt
     1621 ttatgctgat gccgaaggtg tggttgagac tttgagtgaa cgtgggtttc ggctcggtgt
     1681 gatttcaaat tgggatgaca cgctcccacc aattctggat cggtttgggc tcctcacggc
     1741 gtttgaaata ctgattgttt cttctgatct tggcctggaa aaaccagacc caactatttt
     1801 ccagcatgcg ctggaacggg ctgggattga tccagccgca agtattcaca ttggggatga
     1861 ctggcaatgt gatgtggtcg gtgccgctca gaccggaatc caacccatct ggattgaccg
     1921 aggaatgcgc aaagttgtgg ttcagtccac cctgcctgat caaactgaac tcccgatcaa
     1981 accagtcacc atcacgaccc tttcgacagt gaccagcttt ct
//
LOCUS       DAOQDO010001247         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_54988_length_2020_cov_3.023410, whole genome shotgun sequence.
ACCESSION   DAOQDO010001247 DAOQDO010000000
VERSION     DAOQDO010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54988_length_2020_cov_3.023410"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..537)
                     /locus_tag="PKZ53_17700"
     CDS             complement(58..537)
                     /locus_tag="PKZ53_17700"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNJ42327.1"
                     /translation="MTPPDLDQQCNTFAAELETYLSKIVTLGNFELEVRVSVREPHQV
                     WVNFSGEDVPLLLGHNAELLNTLEYLASRVFTRYLRGEIRLRFDAGSYRAHREHELRL
                     MATAAAERVKRFGRPFTLAPMTSFERRIIHTALLEDKSVRTESIGDGEERKVVIHLA"
     gene            complement(622..>2020)
                     /gene="yidC"
                     /locus_tag="PKZ53_17705"
     CDS             complement(622..>2020)
                     /gene="yidC"
                     /locus_tag="PKZ53_17705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNJ42328.1"
                     /translation="LRLAIKSDTQLETILNSVNFRIDPPVTNLTVGPGETKELSFTYE
                     DPTQGIQVRKSLVFQGDEKDGAGYTFDYTVDVKKGQSPVQANTVLGPNFGDQSVHTFD
                     SYHTPPQAVVVTTKPYYRPATDVKPDQFEKNRYQNVHWAALADHYFAMAVIPEQTVPE
                     VVLSNRKVTETVAGTPTERDYVAVLIPLANGVKNTFYIGPKDRNYLLPVNQRLGNRVD
                     LEVLVDYGIFSFLVRPLVPLLDVSVKILHRYTNNYGWAIIAITIIVNTLLFPLKWKSS
                     VALRKTAEIQPRVKEIQDRIKSLPKNDPRVLEGQLELARLMREGNPLMGCLPLLLQMP
                     IFFAFFIYLQISVDIRQEHFISWLNDLSAPDRLHILPIAMCVTQIGSTLLMPSPATDD
                     PAQKMQKTLMTWVFPIVLTYFFFWTAPSGLVLYWMAGNIIGISQQLVINKLNPIQPPP
                     VNTPQKAKKPAESPA"
BASE COUNT          495 a          463 c          506 g          556 t
ORIGIN      
        1 caacttttta ttttcttctc ttccaaaaat ttttcacttt gagagccgtg gtctttttca
       61 ggccaggtga atcacaactt ttcgttcttc accatccccg atgctctcag tccggactga
      121 tttatcttcg agtaaggcag tgtgaatgat acggcgttcg aaggaagtca tgggcgcgag
      181 cgtaaatggc cgaccaaacc gcttgacccg ttccgctgcc gccgttgcca tcaaccgcag
      241 ttcatgttca cgatgggccc ggtaactgcc ggcatcaaac cgcaggcgga tttcaccacg
      301 gagatagcga gtaaagacgc ggctggccag gtattcaagc gtattgagta attctgcgtt
      361 atgaccaagc agcaacggca catcctcgcc tgaaaaatta acccagacct gatgtggctc
      421 ccggactgat actctcacct caagctcaaa attgcccaga gttacaattt tcgaaagata
      481 tgtttccagt tcggctgcaa atgtattgca ttgttggtca agatcggggg gtgtcataat
      541 ccgtgattcc tttgcactga ttcatccaga ttgctggttt caccgtcagc cgattcggtg
      601 acggtgagca gacactgttt tttacgctgg agattcggct ggtttttttg ctttttgggg
      661 tgtgttcacg ggtggaggct gaattggatt cagtttgtta atcaccagtt gttgcgaaat
      721 accaatgata tttccagcca tccagtacag aaccagcccg cttggtgccg tccagaagaa
      781 aaagtaggta agcacaatcg gaaaaaccca ggtcatgagg gttttctgca ttttttgagc
      841 tggatcatca gtggctggtg atggcatcaa cagtgtggag ccaatctggg tcacacacat
      901 cgcaattggc aatatgtgca gccgatctgg tgccgacaaa tcattgagcc aggaaataaa
      961 gtgttcctgg cgaatgtcga ctgaaatctg aagatagatg aagaacgcaa aaaagatcgg
     1021 catttgtagc aggagcggca ggcaccccat cagcgggttc ccctcgcgca tcagccgtgc
     1081 cagttcaagt tgaccttcta agactcgggg gtcgtttttg ggaagacttt taattcggtc
     1141 ctgaatctcc ttgacccgag gttgaatctc ggctgttttt ctaagtgcca ctgacgattt
     1201 ccatttcagt gggaagagca gggtgttgac aataatggta atggcaataa ttgcccatcc
     1261 ataattgttg gtatagcgat gcaggatttt gacggaaaca tccagcaatg gcacaagcgg
     1321 tcggaccaga aacgaaaata ttccataatc aaccaatact tccaaatcaa ctcgatttcc
     1381 gagtcgctgg ttcactggga ggagataatt tcgatctttt ggtccgatgt agaatgtatt
     1441 ttttacgcca ttggccaggg ggatcaagac ggcgacataa tcgcgttcgg ttggggttcc
     1501 agcaactgtt tccgtcactt tccgattgga aagcacgacc tcaggtacgg tttgttctgg
     1561 aatgaccgcc attgcaaaat aatggtcggc cagtgctgcc cagtgaacat tctgataccg
     1621 gtttttttca aactgatcgg gtttaacatc tgtcgctggc cggtaatatg gcttggttgt
     1681 taccacaact gcctgtggcg gtgtatggta gctatcaaat gtgtggacag actgatcacc
     1741 aaaatttggc cctaaaaccg tattggcctg aactggcgac tgaccttttt tgacatcaac
     1801 tgtgtagtca aaagtgtagc cagcgccgtc tttttcatct ccttgaaaga ccagtgattt
     1861 ccgaacctga attccctgag ttgggtcttc ataggtaaac gacagttctt tcgtttcacc
     1921 tgggccaaca gtgaggttgg tcacgggtgg gtcaattcgg aaattcacgg aattcagtat
     1981 tgtttcaagc tgggtatcag atttaatcgc cagccgcagg
//
LOCUS       DAOQDO010001248         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55103_length_2018_cov_2.545084, whole genome shotgun sequence.
ACCESSION   DAOQDO010001248 DAOQDO010000000
VERSION     DAOQDO010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55103_length_2018_cov_2.545084"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..>2018)
                     /locus_tag="PKZ53_17710"
     CDS             complement(333..>2018)
                     /locus_tag="PKZ53_17710"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42329.1"
                     /translation="VGAIFDSIGAATTQGSAYVFVRTGTTWSQQQKLTASDGAATDRF
                     GSSVVISGETVVVGAFSDNIGANGDQGSAYVFVRTGTTWSQQQQLTASDGAANDRFGN
                     SVAISGETVVVGAQLDDIGANTDQGSVYVFVRIGTIWTEQQKLTASDGAPDDLFGGAV
                     AISGETIVVGVNSADIGANSDQGSASVFVRTGMTWTERQKLTASDGVASAFLGSSVAI
                     SGETLVVGAPNDTIGANADQGSASVFVLDCDVMTYPSLLFVADTGNNRIQKFDGDVWT
                     VVAGSLGSGIGQFRVPEAVTADMTGTLIYVADTGNNRVQWSTDSGATWRIFASFGAGP
                     NQVRAPQGLALDSNGNLYVADTGNNRVLRFLGGVPGTGEILLSGGRTNEVSSPQGLAI
                     DEMFNLYVADTGNSRVLKVLNADQPSVIRQTVANSGSSANQVRQAQGVAVDADGNLYV
                     ADTGNNRVLKFVGGEAGMGVVLATIGSAVGQVRGAEGVAVNVFAAGPLAGITVLAVGD
                     TSNNRIQGLDLSSVARPESSLTVGPAIGTWQLLGTPNNIGSQVGKFRSPGKIK"
BASE COUNT          434 a          657 c          481 g          446 t
ORIGIN      
        1 gtttcaccag atggaaattg cacttcgctt tggtctgaag gtgcgggtca gtgcgtgatg
       61 aaataagaca ccacacgaaa aaaacacaaa ggacagaaaa gaaatgagcc tgctttggtg
      121 aagtagtaat tcctggaatc tcaattcttc actccgatac agtctccact cgccggattg
      181 aataattgcc ataccatttt ggcctgaaga tatcgtctta cgtggaggct aattcattga
      241 aaaaatcgta ttttccccag ctttcagtcc gatactctca gcccaaaatg gtatggctta
      301 gtttgttcga ctacctgcag cctggcctgg cactatttga ttttgcctgg gctgcggaat
      361 ttgccaacct ggcttccaat attgtttgga gtgcccagca gttgccaggt gccaatcgcc
      421 ggaccaaccg tcagagatga ctcaggtctg gccaccgaac tcaaatccag gccctggatt
      481 cgattgttgc tggtgtctcc cacggccaga actgtgattc cagccagagg tccagcggca
      541 aacacgttga cggcaacgcc ttcggcaccg cgtacctgtc cgacggcaga gccaatcgtc
      601 gccagcacga ctcccatgcc agcttctccg ccgacaaact tcagtacccg attgttcccc
      661 gtatcggcga catacagatt cccgtcggcg tccaccgcca ccccctgtgc ctggcggacc
      721 tggtttgccg atgacccact gttggcaacc gtctgtcgga tcactgatgg ttgatcagca
      781 ttcagaactt tcagcacgcg actgtttccg gtatcggcca catacaggtt gaacatttcg
      841 tcaatggcca gcccctgtgg gctgctcact tcgtttgttc gaccaccaga taacaaaatc
      901 tctccggtgc ccggcactcc gccgagaaag cgcaggactc gattgtttcc agtgtcagcc
      961 acatacaaat tgccgttcga atccagcgcc agtccttgag gtgcccgcac ctgatttggc
     1021 ccggcgccaa atgaagcaaa gatccgccag gttgcaccac tgtcagttga ccactgaacc
     1081 cggttgttcc ccgtatccgc tacataaatc agggtgccag tcatatccgc tgtcacagcc
     1141 tctggcacgc ggaattgacc aattccagaa cccaggctgc ccgccaccac ggtccacacg
     1201 tcaccatcga atttttgaat ccgattgttg ccggtatcag cgacaaagag gagtgacgga
     1261 taggtcatca cgtcacagtc cagtacaaac acagaggctg acccctgatc ggcgttggca
     1321 ccgatggtgt cattgggcgc cccgaccacc agggtctctc cactgatcgc caccgaactc
     1381 cccaagaagg cgcttgccac tccgtctgaa gctgtcagtt tctgccgctc agtccaggtc
     1441 atcccggtcc gcacaaacac agaagcggat ccctgatcac tgttggcacc aatgtcggcg
     1501 ctgtttaccc cgaccacgat tgtctccccg ctgattgcca ccgccccccc aaacaggtca
     1561 tccggcgccc cgtccgaagc cgtcaacttc tgctgctcag tccagattgt cccgatcctc
     1621 acaaacacat agaccgatcc ctggtcggtg ttggcaccga tgtcgtccag ttgggccccg
     1681 acgaccaccg tctctccgct gatcgccacc gagttcccaa accggtcatt ggccgccccg
     1741 tccgaggccg tcaactgctg ctgctgactc cacgtcgttc ctgtccgcac aaacacatag
     1801 gccgatccct ggtcgccgtt ggcaccgatg ttatcactga atgccccgac gaccaccgtc
     1861 tctccgctga tcaccaccga acttccaaac ctgtcagtcg ccgctccgtc cgaggccgtc
     1921 aacttttgct gctggctcca ggttgtcccg gttcgcacaa acacataggc cgatccctgg
     1981 gtggtggcgg cgccgatgct gtcaaagatt gccccaac
//
LOCUS       DAOQDO010001249         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55135_length_2017_cov_3.333333, whole genome shotgun sequence.
ACCESSION   DAOQDO010001249 DAOQDO010000000
VERSION     DAOQDO010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55135_length_2017_cov_3.333333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..479)
                     /locus_tag="PKZ53_17715"
     CDS             complement(57..479)
                     /locus_tag="PKZ53_17715"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNJ42330.1"
                     /translation="MTSQCIFCDIVNGRELASIVFQDELTLAFMDIRQFHAGHVLVIP
                     RQHFNDVRELDTETGTALMQTVSRLTRAVGLAFPNDGLSLWHSIGPAAFQEVPHLHIH
                     IHPRLPDDNFLRVYPGSPPTSDKATRDQYAELVRARLD"
     gene            500..1006
                     /locus_tag="PKZ53_17720"
     CDS             500..1006
                     /locus_tag="PKZ53_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNJ42331.1"
                     /translation="MNSNIFWITQIGAGRLAIMPRPRGNQFLETEIARFAAENIHVVV
                     SCLTQPEVLAQGLIAEPDLCREQGLTFLSHPIVDHFIPDSMPDTARVIQELHGFLLEG
                     KNVAIHCYAGIGRSALLAGCVLILDGMSVNDAFDVISKARDWPVPEMKKQYDWAVKFA
                     EGCQEPES"
     gene            complement(1043..1651)
                     /locus_tag="PKZ53_17725"
     CDS             complement(1043..1651)
                     /locus_tag="PKZ53_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ42332.1"
                     /translation="MTQERVEPFVSMSLPVALDVHSVQLSDRQFQRLCRDNRDLRFEL
                     TAKGELIIMPPTGALTGWRNAKLTYRLTAWAEADGTGVTFDSSTGFTLPNGAKRSPDA
                     SWIKCERWEALTEKERQGFAPLCPDFVVELRSPEDSLSALQEKMVEYQESGAQLGLLI
                     DPKAKRVYVYRPEQTVEVLENPEAVSCEPLLPSFALSLKDIW"
     gene            complement(1757..>2017)
                     /locus_tag="PKZ53_17730"
     CDS             complement(1757..>2017)
                     /locus_tag="PKZ53_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42333.1"
                     /translation="ETTLSLEVRWYDDWQRWRIGSSDFETLLSGVTRLVHLRGQVLSA
                     MKQILDEHGETGYKQKWCLHKFPIQEFRQLQASSPRSRLVSG"
BASE COUNT          527 a          503 c          463 g          524 t
ORIGIN      
        1 tcattcttca ttcttcattc ttcattcttc attcttcatt cttcattctt catttactaa
       61 tccagccgtg cgcggaccag ttccgcatac tgatcacgtg tagccttatc tgaagtcgga
      121 ggactgccgg gatagacccg caggaagtta tcgtccggga gccgtgggtg aatatggatg
      181 tgcagatgcg gcacttcctg gaacgcagcc gggccaatgg aatgccagag actcaagcca
      241 tcatttggga aagccagtcc aaccgcacgc gtgagacgtg agacagtctg catcaaggcc
      301 gtacctgttt cagtatccaa ttcccgaaca tcattgaaat gctggcgagg aataaccagg
      361 acatgacccg catgaaactg gcgaatatcc ataaatgcca gcgtcagttc atcttgaaag
      421 acgatgctgg ccagttcacg gccattgacg atgtcacaaa aaatacattg gcttgtcatc
      481 gaaacctcat ttcaattcta tgaattcaaa cattttctgg atcacacaga ttggcgctgg
      541 acggctggca attatgccac gtcccagagg caaccagttt ctcgaaactg aaattgccag
      601 gtttgcggct gaaaatattc acgttgtggt ttcgtgtttg acacagcctg aagttctggc
      661 gcaaggcttg atcgccgaac ctgacctgtg tcgtgaacaa ggactcacct ttctttcgca
      721 tcccattgtt gatcatttta ttccagattc catgccagac acagcccgag tgattcagga
      781 acttcacggg tttcttttgg aaggaaaaaa tgttgccatt cactgttatg cggggattgg
      841 tcggtcagca ttactggcgg gatgtgtcct gattttggat ggaatgagcg tcaacgatgc
      901 ctttgatgtg attagcaaag ccagagactg gccagtgccg gagatgaaaa aacaatatga
      961 ctgggcagtt aaatttgctg aaggatgtca ggaacctgaa tcctgatagc attgagtccc
     1021 atcccccaaa ccctgattgg tattaccaga tgtctttcaa gcttaaagca aacgacggca
     1081 aaagtggttc acacgaaaca gcttctggat tctcaagaac ctcaaccgtc tgttctggcc
     1141 gatagacata aactcgtttt gcttttggat caatcaggag tcccaactgt gcaccactct
     1201 cctggtattc gaccattttc tcttgaagag ctgaaagtga atcttcaggc gaccgcagtt
     1261 ccacgacaaa atcaggacag agcggcgcaa acccttggcg ttctttttcg gtcagtgctt
     1321 cccatcgctc acatttgatc caggatgcat caggcgaacg cttggccccg tttggcaaag
     1381 taaatccagt tgaagaatcg aacgtaacgc cagttccatc agcttcagcc caggcagtca
     1441 ggcgataggt caactttgca ttgcgccatc cggttaaggc accagttggg ggcataatga
     1501 tgagttctcc tttggcagta agttcaaatc tcaaatctct gttatcacga cacagacgct
     1561 gaaattgcct gtcactcaac tgtaccgaat gcacatcaag agccacaggc aaggacattg
     1621 aaacaaacgg ctcaactctt tcttgggtca tgggtgcctc acaggtcatg atgggattaa
     1681 ttccctgggt ataatatctg cattatttac catcaactct gattctgcca ttcatccctg
     1741 aatacaattg tgaactctaa ccagagacca gccgggaacg tggagaactg gcttgcaact
     1801 gacgaaattc ctggattggg aatttgtgca agcaccactt ttgtttgtac ccagtttcgc
     1861 catgttcatc caaaatttgt ttcattgccg ataaaacctg cccgcgaagg tggaccagtc
     1921 gggtcactcc agacaaaagc gtttcaaaat cagaactgcc gattctccaa cgctgccagt
     1981 catcatacca gcggacctcc agtgagagag ttgtttc
//
LOCUS       DAOQDO010001250         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55187_length_2016_cov_3.149924, whole genome shotgun sequence.
ACCESSION   DAOQDO010001250 DAOQDO010000000
VERSION     DAOQDO010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55187_length_2016_cov_3.149924"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="PKZ53_17735"
     CDS             complement(<1..1829)
                     /locus_tag="PKZ53_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42334.1"
                     /translation="MANNFFGVFSPIGVIPVAADSFFHMHRIRWATEHGFQVPDVERY
                     LNFPIGAHTYWPYGFDLVYAAFARLITPLHPDPWWTFATASVVTPLISALTPCLVFEI
                     GKTLGSKRLGIWSAFLCVILPSITMTGSIGYVDHHVFESFWLAVYLLAYLKALTLCES
                     HLVKARWLARLTGVAMTAGVLFTTVLPILLPLHTGVVVCQIVREWRKSNTTVIRQLIE
                     LNSFVYTAFAVTLLPFVTLPIAEPNGINPALIFSWLFGFGLASTVLILLNFWKLQTND
                     KPGIPFQWRFGMCLLALGLVLIRTNLSSALSFLEYGIGHLLRGDPWVATVQENLPLWY
                     FPAPRIFGMFSGFVVVWPVAIGLMLKRGWEKSPLLWAVTLLGIVQAGITVLQLRFCGF
                     ATVTYALTLGYLVTAAIEWLYQRFDLKETTTVREIKGLVCFVGGLLIWLGMLWPILAN
                     IVYPTAFIGSVSPMFFRLFPTLQWINRHTPPVSPQANATTDYCIAADWIAGHWIVLMG
                     NRPVLASPMEHTQSHREGIHDGAAALMLPPEAAFHVMERRRIRYLLVTDIQVTTFLEA
                     AYWDLATNQIAFPRSQTETIQQALYFRLLRDAIPANDPAARVLR"
     gene            complement(1819..>2016)
                     /locus_tag="PKZ53_17740"
     CDS             complement(1819..>2016)
                     /locus_tag="PKZ53_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42335.1"
                     /translation="ILKRIRDKESENLVSPAPLSSYHLSPCHLSVCHAAISSNDPTCI
                     LKTRSNLVVDLRLSIFASDGK"
BASE COUNT          570 a          501 c          550 g          395 t
ORIGIN      
        1 cggagcactc tggctgctgg atcattggca ggaattgcat cacgcagcaa ccgaaaataa
       61 agcgcctgtt ggattgtttc ggtctgcgaa cgcgggaatg caatctggtt ggtggcaaga
      121 tcccagtaag cggcttcaag aaaagtcgtt acctgaatat ccgtgaccag aagataccgg
      181 attcgacggc gctccataac gtgaaaggct gcttcgggtg gcaacatcag agcggcagca
      241 ccgtcatgaa tgccttcgcg gtgtgattgg gtatgttcca tcggcgaagc cagcaccgga
      301 cggttgccca tcaggacaat ccagtgacct gcaatccaat ctgccgcgat gcaatagtcg
      361 gttgtggcat tcgcttgggg ggaaacaggc ggtgtgtggc ggtttatcca ttgcaaggta
      421 gggaacagac gaaagaacat tggactgaca ctgccaataa atgcagttgg ataaacgata
      481 ttggccagaa tgggccacaa catcccaagc catatcagaa ggccgccaac aaaacagacc
      541 agtcccttga tttcacgcac agtcgtggtt tctttcaggt caaatcgttg ataaagccat
      601 tcaatcgcag ccgtcaccag atatcccagg gttagggcgt aggtcacggt cgcaaatccg
      661 caaaaccgga gttgtaagac cgtaatccca gcctggacga tgcccaacag ggtgactgcc
      721 caaagcagcg gcgatttttc ccagccccgt ttaagcatca ggccaattgc aactggccaa
      781 accacgacga accctgaaaa cattccgaag atgcgtgggg ctgggaaata ccagagtgga
      841 aggttttcct ggaccgttgc gacccaggga tcaccgcgca acaagtggcc aattccatat
      901 tcaagaaatg acagcgctga cgaaagattg gttcgtatca ggaccaaccc cagagccagg
      961 agacacatcc cgaaccgcca ctgaaaagga atccctggtt tgtcattagt ctgaagtttc
     1021 cagaaattga gcaggatcag taccgtactg gccagcccaa atccaaacaa ccaggaaaag
     1081 ataagcgcag gattgattcc attgggttcc gcaattggga gggtgacaaa cggcagcagc
     1141 gtcacggcaa aagctgtata cacaaaacta ttgagttcaa tcagttggcg aattaccgtc
     1201 gtatttgact tcctccattc gcgaacgatc tgacacacca caacgccagt gtggagcggc
     1261 agcagaatcg gcaaaacggt ggtaaacaaa accccagccg tcatggcaac acccgtcagc
     1321 cgggccagcc agcgggcttt taccaggtga ctttcacata gcgtgagggc tttgagatag
     1381 gccagcaggt aaaccgccag ccagaaggat tcgaaaacat ggtgatcaac atagccaatg
     1441 ctgccggtca tggtgattga cggcaaaatc acgcacaaga aggctgacca gatcccaagc
     1501 cgtttacttc caagtgtttt tccgatttca aacaccagac aaggagtcaa ggcgctgatg
     1561 agtggtgtga cgaccgaggc tgtggcaaag gtccaccacg ggtctggatg caatggggtg
     1621 atcaggcggg caaaggcggc atataccagg tcaaacccat atggccagta ggtgtgcgcc
     1681 ccaatcggaa agttcaggta gcgctccaca tccggaacct gaaatccatg ctcggtcgcc
     1741 caccggatgc ggtgcatatg aaaaaaagaa tcagccgcta ccgggataac accgattggt
     1801 gaaaagacgc caaagaagtt atttgccatc cgaagcaaaa atgctgagac gtagatccac
     1861 aaccaggttt gagcgggttt tgaggatgca ggtggggtca ttggaagaga tggcagcgtg
     1921 acagacagat agatgacagg gtgacaaatg ataagatgac aggggggcag gggacaccag
     1981 gttttctgat tctttatccc gtatcctttt cagaat
//
LOCUS       DAOQDO010001251         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55317_length_2014_cov_2.389995, whole genome shotgun sequence.
ACCESSION   DAOQDO010001251 DAOQDO010000000
VERSION     DAOQDO010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55317_length_2014_cov_2.389995"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1233
                     /locus_tag="PKZ53_17745"
     CDS             76..1233
                     /locus_tag="PKZ53_17745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ42336.1"
                     /translation="MPLQGIKYISALPPSGLAIAAQRNILALIQQGIPVTWSPVGFNE
                     QLKLDFLGRLENQEAYRDLFPYLHLPIAYDVVIVHTSPEFYPVVRAREPGKKIIGYSV
                     WETDQIPEGWLPGCQVIDQLWVPCEWNQRVFAARHLPCPIEVIPHIAEDPATPWADFS
                     LGGIRKQDFVFYTIGVWSYRKGIDLTIKAFLRAFSKSDQAVLIVKTCERNLSLSVAGR
                     HFMRSRVSVQLMQKLYGSSSPIFLIDQELSHAEIRGLHQRGDCFVSLTRGEGWGLGAF
                     DAAAIGNPVIMTGHGGQTDYLHRELAYLVDFQLQVARATGFERDIFPPPHQLAVPDLT
                     HAVRVMRRVYEHKQEAQTKARRLQTDIQQRFNAQVIGQKMVSALERDKVTR"
     gene            1436..>2014
                     /locus_tag="PKZ53_17750"
     CDS             1436..>2014
                     /locus_tag="PKZ53_17750"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42337.1"
                     /translation="MIPNLFHFVFGLKPQTRPFHLVFYLCLESCRQVNQPEQIFFYYH
                     HEPFGAYWELIRPHLTLVKVDPSRIVSQHRYDNSMVKKFSYAHHADFIRLEKLLARGG
                     VYADIDTLFVNPIPPQLFSKPFVIGREADVVDLQTGRAHPSLCNAFLMAEPQAAFGRR
                     WLTEMATVFNGEWSRHSCQLAWELSQRDPDEVH"
BASE COUNT          467 a          525 c          491 g          531 t
ORIGIN      
        1 acttcttcag cccgacttct tcagccccga gcttgcgagt cttcagcccg acttcttcag
       61 cccggtgtcc tcactatgcc tcttcaagga ataaagtaca tttctgccct gccaccatcg
      121 ggtctggcga ttgcggccca gagaaatatt ctggccctca tccagcaggg gatcccggtt
      181 acctggagcc cggttgggtt taatgagcaa ttaaagctgg attttctggg aaggctggaa
      241 aaccaggagg cgtatcgtga cttgtttccc tatctgcatc tcccgattgc ctatgatgtg
      301 gtgattgtac acacttcccc ggagttttac ccggttgtca gagcccgtga acctggaaaa
      361 aaaattattg ggtacagcgt gtgggaaaca gaccagattc ctgaaggctg gctgcctggg
      421 tgtcaggtca ttgaccagct ctgggtcccc tgtgagtgga atcaacgggt ctttgccgcc
      481 cgccatctgc cttgtccaat tgaagttatc ccgcacattg ccgaagaccc agccaccccg
      541 tgggcagatt tttcactggg cggcattcgc aaacaggact ttgtgtttta caccatcggg
      601 gtgtggagtt atcgcaaagg aattgacttg accatcaaag cttttctgcg agccttttcc
      661 aaatcagacc aggcagtgct gatcgtcaaa acgtgcgaac gaaatctgtc gctttcggtt
      721 gcgggcaggc atttcatgag gtctcgcgtt tcggttcaat tgatgcaaaa actgtatgga
      781 tccagctctc cgatatttct cattgatcaa gaattatcgc acgcagaaat tcgtggcctc
      841 caccagcggg gggattgctt tgtttcgctc actcggggag aaggatgggg gcttggagcc
      901 tttgacgcgg cagccatcgg gaatccggtt atcatgaccg gtcacggagg gcaaactgac
      961 tatctgcatc gcgaactggc ctatctggtt gattttcaat tgcaggtggc acgggcaacc
     1021 gggtttgaac gcgatatttt tcctcctcct catcaactgg ctgttcctga cctcactcat
     1081 gccgtcagag tgatgcgccg ggtatatgaa cataagcagg aagctcaaac caaagcccgg
     1141 cggttacaaa ctgatattca gcagcgattc aatgcccagg tgattggtca aaagatggtc
     1201 agtgccctgg aacgtgataa ggtgacaagg tgatacagaa attatactgt tttggaatga
     1261 aacctcgtct gataggaaga cgaacccatt gaaaaaattg tatttccctc agcccaaccc
     1321 ctgagcccca aatggtattc ttcacggaag cattcacctt ttcaggattt ttgtcctttt
     1381 caccgctcgt ccttttgttt tctttcaaaa ccttaaaccc tgaacctcaa acctgatgat
     1441 ccccaacctg tttcactttg tttttggtct caaaccacaa accaggccat ttcacctggt
     1501 attttacctg tgcctggaat cgtgtcggca ggtcaaccag ccggagcaaa tttttttcta
     1561 ttaccaccac gagccgttcg gcgcatattg ggaactgatt cggccccact tgacgctggt
     1621 caaagttgat ccttcacgca tcgtcagcca gcatcggtat gacaattcaa tggtaaagaa
     1681 atttagctat gcccaccatg ccgatttcat tcgattggaa aaattgctgg ccagaggcgg
     1741 ggtatatgcc gatatagata ccttgtttgt caatccgatt ccaccacagc tcttttccaa
     1801 accatttgtg attggtcgcg aagctgacgt ggtggacctc caaactggcc gtgcccatcc
     1861 atcactgtgc aatgcctttt taatggctga accacaggca gcgtttggcc ggcgatggct
     1921 caccgaaatg gcaaccgtgt ttaatggcga atggagccgg cattcgtgtc agttagcctg
     1981 ggaactcagt caacgcgacc cagatgaagt ccat
//
LOCUS       DAOQDO010001252         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55391_length_2012_cov_5.519162, whole genome shotgun sequence.
ACCESSION   DAOQDO010001252 DAOQDO010000000
VERSION     DAOQDO010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55391_length_2012_cov_5.519162"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKZ53_17755"
     CDS             complement(<1..303)
                     /locus_tag="PKZ53_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011324566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ42338.1"
                     /translation="MATQQNHHRVIIVGSGPAGWTAAIYVARANLTPLVFEGEASETG
                     EVPGGQLMITTDVDNFPGFPNGILGPELMHNMKTQALRFHTQVISRRVTAVDLSVRP"
     gene            647..1768
                     /locus_tag="PKZ53_17760"
     CDS             647..1768
                     /locus_tag="PKZ53_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ42339.1"
                     /translation="MPRTPRKGSRPKRLLITGIAGGLATQVASAVPPDWEVIGIGRRP
                     PQETLKRDIIFKQLDITKKTLEDLFRHEHFDAVLHLAVANDQHLPMKIRHTVNVIATM
                     RLLDCCERHKIKEVILLSTAEVYGADPTNPIYLPEDSPLKAIQRFTDLGDKVEFDTYC
                     RSWMYHVKDIKTTLLRPCYIVGPHVQNFLTTYLRYGIVPIPMGYDPMIQVIHERDMVE
                     ALLRVLAKPKRGVYNVTGPGEVPLSVAIREAGGIAVPVVYALASPLMKLGWWVGISPF
                     PAPQFDYLMYPCVIDGRKFQEDFQFKPEYNLHETLRTLRRAAVQMDRLDSMHHREEEE
                     PEEEEELEEAEETRPHRSSDSGLKGIPRGRRQNLARPNQ"
BASE COUNT          546 a          530 c          478 g          458 t
ORIGIN      
        1 tgggcgaact gaaaggtcaa ccgccgtgac tcgccgggaa atcacctggg tatggaaccg
       61 gagcgcctgg gtcttcatgt tgtgcatcaa ttccggtccc aaaattccat tcgggaaccc
      121 tgggaaatta tcgacatcag tggtaatcat gagctgtcca cctgggactt caccagtttc
      181 gctggcttca ccttcaaaca ccagcggtgt caggtttgcc cgtgccacat agatagctgc
      241 tgtccaccca gccggacccg atccaacaat aatgacgcga tgatgatttt gttgtgttgc
      301 caagatgccc ccaatctcac gcctgacgct gtcacacttt gtttgagcga gcattttccg
      361 actggacagg tcagcgaaat tcagtaaatg atgatgattc agaatgaaaa aaaccgccca
      421 cattctatgc aaccacgaga agatagcaag acgctgtgcc tttttaaggc gggctgaaga
      481 aatcgggctg aagaaatcgg gctgaagact ttgggcttga ttcctggttt ttcgcgcttt
      541 gtttttcgtg cttctttctt cgaaaatatt gagttctaag caccaggtac caggtaccca
      601 agcaatctcc aatctaacac ttacgtttct ttcgatcaaa aaacatatgc cgcgaacacc
      661 acgcaaaggt tctcgtccaa aacgtctgtt gatcactggt atcgcgggtg gtctcgccac
      721 gcaagtcgcc tcggcagtac caccagattg ggaagtcatc ggcattggcc gccgaccgcc
      781 acaggaaact ctcaagcgcg atatcatttt taaacaactc gacatcacca aaaaaacgct
      841 tgaggatctc ttccgtcacg aacactttga cgcggttctc catctggcgg tcgccaatga
      901 ccagcatttg cccatgaaga tccgccacac cgtcaatgtg attgccacca tgcggctgct
      961 tgattgttgc gaacgccata aaattaagga agtgatcctg ctcagtaccg ccgaagtcta
     1021 tggcgccgac ccaaccaatc ccatttacct gccggaagac agcccgctca aggcgatcca
     1081 acgctttacc gacctgggtg acaaagttga atttgatacc tattgccggt cctggatgta
     1141 tcacgtcaag gacattaaga ccacgttatt gcgcccgtgc tatatcgtcg gtccacacgt
     1201 ccaaaacttt ttgaccacct atctgcgcta tggaattgtg ccaatcccaa tgggctatga
     1261 cccaatgatc caggtcattc acgaacgcga tatggttgag gctctgctcc gggtactggc
     1321 caaaccgaaa cggggcgtct ataacgtgac tggccctgga gaagtaccac tctcagttgc
     1381 gatccgtgaa gctggcggca ttgccgtccc agtggtgtat gcgctggcct caccgctgat
     1441 gaagcttggc tggtgggttg ggatttcacc gttcccagct ccgcaatttg actacttgat
     1501 gtacccatgt gtgattgacg gtcgaaagtt ccaggaggat tttcaattca aacctgaata
     1561 caacctccat gaaaccctgc ggacgctgcg ccgggcagcc gtccaaatgg atcgacttga
     1621 ttcaatgcac caccgggaag aggaagaacc cgaagaagag gaagaactgg aagaagccga
     1681 agaaactcgc ccccaccgct catctgactc tggcctcaaa ggtattcctc gtggtcgtcg
     1741 gcaaaacctg gcacggccaa accaatgatg aatgatgaat gaagaattga gaatgaagaa
     1801 tgaagaattg agaatagaca ttatcggtga taaggaaatc acgattgtaa gtgcttttaa
     1861 atcaatagtc agaatgaggc aaaaacccta actccgatga tgtctaatgc agaaagtgtt
     1921 tgatgcttga tgcctggcaa ccaatacctt cgaagaacca agaaccaaga accaagaacc
     1981 aagaaccaag aaccaagaac caagaaccag gc
//
LOCUS       DAOQDO010001253         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55432_length_2011_cov_2.741820, whole genome shotgun sequence.
ACCESSION   DAOQDO010001253 DAOQDO010000000
VERSION     DAOQDO010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55432_length_2011_cov_2.741820"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PKZ53_17765"
     CDS             <1..1027
                     /locus_tag="PKZ53_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HNJ42340.1"
                     /translation="HAQQPLPVGIPGEIYIGGMGVARGYLNRPDLTEERFKAGVRVEK
                     TGFRVPGSGFRVSEPEVTSQVIYPQIQPPSQSFEEHSEPHNVEPGTRNPEPGTRSLYR
                     TGDLGRWLENGTLEFLGRIDNQVKIRGFRIELGEIEAVMQSVQGVLKAVVLADKSSTG
                     EARLVSFVVLDQMIPGDAVLPALTAYLKERLPEYMLPAVITPLDSLPLTVSGKIDQRA
                     LLGQNLSRPIQAGNYVAARTPIEEELVELWSKLLKIEQIGVYDNFFELGGHSLLLTRL
                     ASQIREQFGVELPLRILFDAPTIDSMTSAIADYQLSQTDSEEAAELLAEIQHLSPEEI
                     RALLEDE"
     gene            1381..1935
                     /locus_tag="PKZ53_17770"
     CDS             1381..1935
                     /locus_tag="PKZ53_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42341.1"
                     /translation="MFTSDKGKLLAAGLALTMFGGAVGAFTTRSNTSTTPAPAPAQTA
                     AAPTTPSQPVPQPTDPMQPQIIMPDQLQPGMTYQLVPVQGTQAAPVQYVEEPQPVTRR
                     TTTRTRRVSSSGAQRTYYTYSEAPQRKPSFWNRHRDILTVGIGTGVGAAIGGLAGGKK
                     GAAIGALAGGGGSALYTYGLRKRN"
BASE COUNT          497 a          545 c          507 g          462 t
ORIGIN      
        1 ccacgcccag caaccgctcc cggtgggtat tccaggtgaa atttacattg gcggaatggg
       61 ggttgcccgt ggctacctca accgacccga cctgacggaa gaacggttta aagcaggggt
      121 cagggttgaa aagacagggt tccgggttcc gggctccggg ttccgggttt cagaaccaga
      181 ggtgacgtca caagtgatat atccccagat tcagccccca tcccagagtt ttgaggaaca
      241 ttcggaacct cacaatgtgg aacccggaac ccggaacccg gaacccggaa cccggtcttt
      301 gtatcggacg ggcgatcttg gtcgctggct tgaaaatggc acgctggaat ttcttggacg
      361 cattgacaat caggtgaaaa tccgtggttt tcgcattgag ctgggtgaaa tcgaagccgt
      421 aatgcagagc gtgcaaggtg tgttgaaagc agttgtttta gctgacaaat cttcgacggg
      481 tgaagcccga ctggtgtcat ttgtggtgct tgaccagatg atacccggag atgccgtcct
      541 gccagcctta accgcctatt taaaagaacg gttacctgaa tacatgctcc cggcagtgat
      601 tactccgctc gatagccttc cactcaccgt cagcggcaaa attgaccaac gggcgctcct
      661 gggtcaaaac ctgtcgcgtc cgatccaggc cggaaattat gtggctgccc gcaccccgat
      721 tgaagaagag ctggtcgaac tctggagtaa gctcctcaaa atcgaacaaa tcggcgtgta
      781 tgacaatttc tttgaactgg gcggacattc cttgctttta acccggctcg cctctcaaat
      841 tcgcgaacag tttggggttg aactcccact tcgaatcctg tttgacgccc caaccattga
      901 tagcatgacg tcagctatcg ccgactatca gctttcccaa actgatagtg aagaagcggc
      961 ggaactcctg gccgaaatcc agcacctgtc acccgaagaa atccgcgctt tgctcgaaga
     1021 tgagtagcgc gaaaagtgag tcgcgagttg cgactcactt ttctactgga tatgcgccat
     1081 tgactacacc atatcgtgca ggactctttt ctttcccctc tcggattttc cgctaaaact
     1141 gacttttcgg tttccaaaac tggattttta tagcaataca gcagctacta tttatccagc
     1201 agcaagtttg gcacaccgct tgttatagag tatcccatca aacacattcc acagaagcaa
     1261 cctggaccct aactgctctt tccaatgaaa gaacagttaa ccatctgtgg aatatactct
     1321 tactcaattt aacacgggat gtctctgaca tattttcaaa accaaatagg agaatccaat
     1381 atgtttacaa gtgataaagg caaactgctt gcagccggtt tagcactgac aatgtttggg
     1441 ggtgccgttg gggcattcac aactcggtca aatacctcaa ccacaccagc accagcgcca
     1501 gcgcaaactg cggcggcacc gaccacacca tctcaaccgg tacctcagcc aactgatcca
     1561 atgcagcctc agattattat gccggatcag cttcagccgg gtatgaccta tcaactggtt
     1621 ccagtgcaag ggacacaagc tgctccggtt caatatgttg aggagccgca gcctgtgacg
     1681 cgtcgaacca ctacccgaac ccgtcgtgtc tcatcttcag gggctcaacg gacctattac
     1741 acctattcag aagctccgca acggaagcct tcgttctgga atcgccaccg tgacattttg
     1801 acggttggca tcggaacggg cgtcggcgcc gccattggtg ggctggctgg tgggaagaaa
     1861 ggtgcggcca ttggggccct ggccggtggt gggggctcag ccctttacac ctatggcctc
     1921 cgcaaacgca actaaccacc ggggctgaaa caaccagggc tgaagaagtc gggctgaaga
     1981 agtcgggctg aagaattggt tttatttcat c
//
LOCUS       DAOQDO010001254         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55477_length_2010_cov_3.231714, whole genome shotgun sequence.
ACCESSION   DAOQDO010001254 DAOQDO010000000
VERSION     DAOQDO010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55477_length_2010_cov_3.231714"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1932
                     /locus_tag="PKZ53_17775"
     CDS             <1..1932
                     /locus_tag="PKZ53_17775"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNJ42342.1"
                     /translation="EGTDKFFTYFQQLISGQPAQNERETFVMKGICIGSMAGNEAPKS
                     GSYLMPNIQRLDNGSYRMYYNRSTFGDDQIKYAESPDGITWTVKGIVLKGSSNPTDLT
                     FAFGGPSVVRLADGRYRMYIRCSPKQEPNMPPKHHIISAISNDGVTFTLEPGVRIEIA
                     SNDPTSQFQLAGHGAFYRLKDGTFAAIFSANPVSTSNQPSDLYLGTSPDGLTWGNFKM
                     LYKGFHDPTVVEKDGKFYLYTMYLHFFHARGTSEDGITWPETLDEILLADDTGTDLTV
                     ARAGVGDLGAAIAPDGNIRIYSNYGNPSEQIAYFDLKPSSSTSDNPPTVRVNSPNGGE
                     QLAINAPLSISWASSDDKGISSQTIALSSDGGGTFPTTLATGLSGSATSFSATLSSSV
                     SSTTRARVRVTATDSSGKTGSDTSDADFSITAPNQADTVAPTVQLVSPNGGEQATAGA
                     NLMIQWNSSDNVGVRSQTIHLSTDGGTAFAIQLATGLPGNATSFAAQLPSTLATTRGR
                     VRVSATDQAGNNGSDTSEADFTITVPVPDDTTPPSVRLIAPNGGEKLKLNQSFLIQWQ
                     SSDNLELASHDLFVSTDGGVSYPNLIATGLPAAAQSFAFTVPSNMAKTKMAKVRVVAV
                     DKAGNRGQDDSDATFRIKK"
BASE COUNT          524 a          513 c          505 g          468 t
ORIGIN      
        1 gaaggtactg acaaattctt tacttacttt caacaattga tcagtggaca acctgcccaa
       61 aatgagcggg aaacgtttgt gatgaaaggc atctgtattg gttcaatggc tggaaatgag
      121 gcaccaaaaa gcggttccta ccttatgcca aacattcaac gcctggataa cggcagttat
      181 cgaatgtatt acaatcgctc aaccttcggt gatgatcaaa ttaaatatgc tgaatcacca
      241 gacggtatta cctggaccgt caaagggatt gtcttaaaag gatcttcaaa tcccacagac
      301 ctcacctttg cctttggggg gccaagtgtc gtcaggctgg cagatgggcg gtatcggatg
      361 tacattcgat gttcacccaa acaagagcca aatatgccgc ccaaacacca catcatcagt
      421 gcaatttcaa atgatggtgt tacctttacc ctggaaccag gtgtccggat tgaaattgcc
      481 tccaacgatc caaccagcca gtttcagtta gccggacatg gtgcatttta tcggcttaaa
      541 gatggcacct ttgcggctat cttttccgca aatccggtca gtacctcgaa ccagccaagt
      601 gacctgtacc tggggacatc gccagatggc ctgacctggg gaaatttcaa aatgctctat
      661 aaagggttcc acgatccgac agtggtggaa aaagatggaa agttctatct ctacaccatg
      721 tatttgcatt tctttcacgc cagaggcact tcggaagatg gtattacctg gccggaaaca
      781 cttgacgaga ttttgctggc tgatgatacg gggacggatc tgactgtggc ccgggccggc
      841 gtcggggacc ttggcgccgc gattgcaccg gatggaaaca tccggattta ttcaaattat
      901 ggcaatccat ccgaacaaat tgcctacttt gatttgaaac cttcatcgtc tacgtcagac
      961 aacccgccaa ccgtgagagt caattcacca aatggtggtg aacaactcgc catcaacgct
     1021 ccgctctcca tttcgtgggc cagttcggat gataaaggga tcagttccca gacaattgca
     1081 ctttcgagtg atggtggcgg aacattccca accacgctgg caaccggtct gagcggatca
     1141 gccaccagtt tttcagccac actctctagc tccgtcagtt caaccacccg ggcgagggta
     1201 cgggttaccg ccaccgatag cagtggaaag accggatccg atacgtctga cgctgatttc
     1261 tcaattacgg cgccaaatca ggcggataca gtggcgccaa ctgtccagct tgtgtcaccc
     1321 aacggaggcg aacaggcaac agccggggca aatttgatga ttcagtggaa ttcaagcgat
     1381 aatgttgggg ttcggtccca gaccattcat ctctcaacgg atggaggcac cgcgtttgca
     1441 atccagctcg ccaccgggtt gcccggcaat gcgacttcgt ttgccgcaca acttccttca
     1501 actctggcga cgactcgcgg cagagttcgg gtctcagcca ctgatcaggc cggaaacaat
     1561 gggtcagata cgtcggaggc tgattttacc attacagtcc ccgtgccaga tgacacaaca
     1621 ccgccttcgg tgcggctgat tgctccaaat ggcggtgaaa aattgaaact caatcaatcg
     1681 tttttgattc agtggcagtc gagtgacaat ctcgaactgg ccagccacga tctttttgta
     1741 tcaaccgacg gaggtgtcag ctacccaaat ctcatcgcga ctggattacc ggcagcagcg
     1801 caatcgtttg cctttactgt tccatcaaac atggcgaaaa caaaaatggc aaaggttcgg
     1861 gttgtggcag tggataaggc tggaaatcgt gggcaggatg acagcgatgc gaccttccgc
     1921 attaagaaat aaaggcagca tgaagaattg agaatgaaga atgaagaaca catcgggttc
     1981 cgggttccgg gttccgggtt ccgggttccg
//
LOCUS       DAOQDO010001255         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55479_length_2010_cov_3.116624, whole genome shotgun sequence.
ACCESSION   DAOQDO010001255 DAOQDO010000000
VERSION     DAOQDO010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55479_length_2010_cov_3.116624"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKZ53_17780"
     CDS             <1..367
                     /locus_tag="PKZ53_17780"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ42343.1"
                     /translation="ASTFAYSQPVIGDIDGDTKPEILFSGSSLTPFTDRNYYLFAVHS
                     DGKPYTGWPKAIIGPRLVPYIWSGFALGDLDGDGKIDGVIPAVERVYAFHFPGAYRPE
                     NVPWGTFQQNSQRTGALKK"
     gene            412..1920
                     /locus_tag="PKZ53_17785"
     CDS             412..1920
                     /locus_tag="PKZ53_17785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family serine peptidase"
                     /protein_id="HNJ42344.1"
                     /translation="MKRLILFFGLLCLLLVMATGVWAESPQPEAVITVNIHVRLRDEA
                     GLRALVEQQQTPGSAVYRRWLTHDEFRRRFAPTTESCDRVEAWAKAAGLKIASRINDR
                     SLLVCQAPASVIRKVFAPTLAGFQAARAAGQRVAVTQLPAGLASDVSVVEGLDGVDLY
                     HSPQAEQQAIKANTNGQNCDLRQLLYPQDLWSLYNLNPLYSVGIKGNGQKVALVMAGA
                     VAPADLTMFQQSYNLPVRAVEPHSVGLGTPGAAFNGAMAEVEYLSALAPEALIQAVQV
                     AASGSGDVTATNITAAIGYVVDRLRDTKVVATSLGICEQALSPATIQAQEALLLQAAA
                     QGQTWVVASGDNGSDGCRNNGSSVFYPATSPNVTAVGGTELLPIRDSEGKVIRYDAEI
                     AWNNDAGASGGGTSLVFEKPAYQSRMTILPGRKRAIPDVSFHGGGNLVVILNGVRTCY
                     AGTGVPAAAWAAIFTLVISKSVGADSGRLTYRCTTLAFSRRQEPGQKSIVMC"
BASE COUNT          439 a          502 c          605 g          464 t
ORIGIN      
        1 ggcctcgact tttgcctatt cacaaccagt gattggggat attgacggag acaccaaacc
       61 ggagattctg ttttctggct caagtttgac gccatttact gaccgaaatt actacctgtt
      121 tgcggttcat agcgatggga aaccctatac gggctggcca aaagcaatta ttgggccgcg
      181 gttggttcct tatatctggt cagggtttgc gttgggggat ctggatggag atggcaaaat
      241 agatggagtc atcccggcgg ttgagcgcgt gtatgcgttt catttccctg gcgcctatcg
      301 cccagaaaat gttccctggg gaaccttcca gcaaaattcg cagcgcactg gcgccttgaa
      361 aaaataaacc cgcccaataa agcggtatgt accggaatgg aagtcattgc tatgaagcga
      421 ttaatactct tttttggatt actgtgcctg ctgcttgtga tggcaacagg tgtttgggcg
      481 gaaagtcctc aacctgaagc ggtgatcacc gtcaacattc atgttcgatt gcgtgatgaa
      541 gctggcctga gggccctggt cgaacaacaa caaaccccag gttctgctgt ttaccgacgc
      601 tggctcaccc acgatgagtt tcgccggcgt tttgccccga ccactgagag ctgtgatcgg
      661 gttgaagcct gggcgaaagc cgctgggctc aagattgcct ctcggatcaa tgatcggtcg
      721 ttgctggtgt gtcaggcgcc ggcatcggtc atccggaaag tgtttgcccc gaccctggct
      781 ggttttcaag cggcacgggc tgctggccaa cgcgtcgctg tgacgcagtt gcccgctggg
      841 ttggccagtg atgtcagtgt ggttgaggga ttggatgggg ttgatctgta ccactcaccg
      901 caggctgagc aacaggcgat caaagccaat accaatggtc aaaattgcga tttgagacaa
      961 ctcctgtatc cacaggattt gtggtccctc tacaatttaa acccgctcta tagtgttggt
     1021 atcaaaggca atggtcagaa agtggccctg gtcatggctg gtgccgtggc gcctgctgac
     1081 ctgaccatgt ttcaacagag ctataacctg ccggtccgag cggttgaacc tcactccgtt
     1141 ggacttggga cacccggagc ggcctttaac ggcgccatgg ccgaagttga atatttgtcg
     1201 gcactggcac ccgaagcact gattcaggca gtacaggtcg ccgccagtgg aagtggcgat
     1261 gtgaccgcga ccaatatcac ggcggcgatt gggtatgtgg ttgatcggct ccgggatacc
     1321 aaagtggtgg ctaccagctt aggaatttgt gaacaggcat tatcgccagc cacgatccag
     1381 gctcaggaag cactgctgtt gcaagcggcg gctcaggggc aaacctgggt ggtggccagc
     1441 ggagacaacg gctccgatgg gtgtcgcaac aacggtagtt ccgtgttcta tccagccacg
     1501 agcccaaatg tcacggcggt tgggggaacg gaactgctgc cgattcgaga cagcgaggga
     1561 aaagtcatcc gctatgacgc ggaaattgcc tggaataatg atgctggcgc cagcggcggc
     1621 ggtaccagcc tggtatttga gaaaccagcc tatcaatcgc gcatgacgat tttgccgggc
     1681 cggaaacggg ccattccaga tgtatcgttt cacggcggcg gaaatctggt ggtcatcctg
     1741 aatggagtcc gaacctgtta tgccggaacc ggagttcccg ccgccgcctg ggcggccatt
     1801 tttaccctgg taatcagcaa atcggtggga gcggactcgg ggcggttaac ctaccgctgt
     1861 acaaccttgg cattcagcag gcggcaggaa ccgggccaga agtctatcgt gatgtgttaa
     1921 ttgggaataa ctttgtcgga aacagcatcg gagatgtaac tggtccaggg tttgatcctg
     1981 taactgggtg gggcaccttt gatggggaac
//
LOCUS       DAOQDO010001256         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55517_length_2009_cov_3.204197, whole genome shotgun sequence.
ACCESSION   DAOQDO010001256 DAOQDO010000000
VERSION     DAOQDO010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55517_length_2009_cov_3.204197"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1363)
                     /locus_tag="PKZ53_17790"
     CDS             complement(110..1363)
                     /locus_tag="PKZ53_17790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ42345.1"
                     /translation="MTAFQPGSNTFKYRIPIQWRRIAFVALVIGILLANYTLVGLSTS
                     LAQSRTGRPPSNQKKNPDIPEKEPEPDVKQEEPEKTPSQGTLRSKAPGRRPPLKRDKP
                     QTDTDEPIVIETNVVNTEVVVYNKKTGAIYQNLKKENFTIIEDGVKQEITNFRPVEAP
                     VTMVMVLEYSRIISGIRIEVLQAAGAFLQYFVKPEDYISIVAYDIRPKVLNDFSNNPS
                     ELIGSVNLLWRNVPAFSESNLYDTLRFVIKGGQLDGEEYVGLEEVQGRTAILLVSLGI
                     DTFSKLNFDEARRMVDRAGIPVYSIGIGNLFYKMNDFRMPPEQNLTFLQAFNTLRTFS
                     DSTGGKYFPVTFQGELPTTMKSISALMRNQYSLGYEPSNTRREGKRRKIELQVDVNND
                     GKPDNKELELQYRKTYTEPGGKPDK"
     gene            complement(1552..>2009)
                     /locus_tag="PKZ53_17795"
     CDS             complement(1552..>2009)
                     /locus_tag="PKZ53_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42346.1"
                     /translation="CCILNRVAFVGTRGWQCPGTDSSLDLMERIEQSGREAPDTPREP
                     APHRGYTDQDLKIYQREVGRLRIGLEQAHRRAAEFDHLVVILHYPPMNSQHEPSGFTD
                     LLDEYQATWCVYGHLHGEAIATAFNGQRNATRYQLVSADSIHFTPFRLF"
BASE COUNT          457 a          479 c          519 g          554 t
ORIGIN      
        1 ttggtgcttg gttcttggtg cttggtgctt ggttcttggt tcttcgaagg tattgagtcc
       61 taagcactaa gtaccagaca ctaagcacca ggtaccaggt accaaattct tatttatccg
      121 gtttgccacc cggttcggtg taggttttgc ggtactgcag ctcaagttcc ttattgtcag
      181 gtttcccatc attgttgaca tcaacctgca gttcgatttt gcggcgtttg ccttcgcgcc
      241 gggtgttgct cggttcatac ccaaggctgt actggttgcg catcagggca ctgatggact
      301 tcatcgtggt tggcaattcc ccctgaaagg tcaccgggaa atatttgccg ccagtggagt
      361 cagagaatgt ccgaagtgtg ttaaacgcct gcagaaacgt cagattttgc tcgggtggca
      421 tccggaaatc attcatttta taaaacaaat tcccgatacc aatgctatag accggaatcc
      481 cagcccgatc aaccatccgg cgggcttcgt caaaattgag cttgctgaac gtgtcaatcc
      541 ccagactgac cagcagaatt gcggttcgtc cctgaacttc ttcaagtccc acatattctt
      601 cgccatcgag ttgccccccc ttgatcacaa atcgcaacgt gtcatagaga ttgctttccg
      661 aaaatgccgg aacattgcgc cagaggaggt tgacactccc gatgagttct gatggattat
      721 tagaaaagtc attgaggact tttggccgga tgtcataggc aacaatcgag atatagtctt
      781 ctggcttgac aaaatactgc agaaacgcac cagcggcttg caggacttcg atgcgaattc
      841 cagagataat ccggctgtat tcgagcacca tcaccatggt gactggggct tcaaccgggc
      901 gaaagttggt aatttcttgc ttcacgccat cttcaatgat ggtgaaattc tcttttttga
      961 ggttttggta gatggcaccg gttttcttgt tatagacgac cacttccgtg tttaccacat
     1021 tggtttcgat cacaatgggt tcgtcggtat cggtttgagg cttgtcacgc ttgagtgggg
     1081 gacgacgtcc aggagctttg ctgcggaggg ttccctgtga aggtgttttt tccggttcct
     1141 cctgtttgac atctggttcc ggctcttttt caggaatgtc cgggtttttc ttctggtttg
     1201 atggaggtcg accagtccga gactgagcca acgacgtgga aagaccaacc agtgtgtaat
     1261 tggccagcaa aatcccaatc accagcgcca caaacgcgat tcgtcgccac tggatgggaa
     1321 tgcggtattt gaaagtattg ctaccgggct gaaatgctgt cacagaaaaa cctcctcatt
     1381 gtcagtagaa cttagtcagt agtcagtagt caggcgttgg ggttttgaag tatttgtcaa
     1441 gtgtattttg tttggtctaa gttatttagg aacaattttt ctcaagagtt ccacgaattc
     1501 agcgacccga gtttttctac tgactactga ctactgacta cggactagga actaaaataa
     1561 ccgaaatggg gtgaaatgaa tgctgtcggc actgacgagt tgatatctgg tggcgttgcg
     1621 ctggccgttg aaggcagtag caatggcctc gccgtgcaaa tggccataga cgcaccaggt
     1681 ggcctggtat tcgtccagca agtcagtaaa gccgctgggt tcgtgctggc tgttcattgg
     1741 cgggtagtgc agaatcacca ccaggtggtc aaattcggcg gcccgtcggt gtgcctgctc
     1801 taatccaatg cgcaatcggc caacttctcg ctggtaaatt ttgagatctt gatcagtata
     1861 gccccgatgt ggggcaggtt cacgaggtgt gtcaggtgct tcacgcccgg attgctcgat
     1921 tcgttccatc aaatcaaggc tggagtccgt tcctggacac tgccagccac gcgtcccaac
     1981 aaaagccacc cgattgagaa tacaacaac
//
LOCUS       DAOQDO010001257         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55579_length_2008_cov_2.575525, whole genome shotgun sequence.
ACCESSION   DAOQDO010001257 DAOQDO010000000
VERSION     DAOQDO010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55579_length_2008_cov_2.575525"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKZ53_17800"
     CDS             complement(<1..307)
                     /locus_tag="PKZ53_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42347.1"
                     /translation="MLTNHLQRLESIDPIRSLGTITRAVGLVVESKGPFVSVGDLCYL
                     DHQSNHPSAPESLLEVVGFRDSTILLLPLGQMPQIRPGDRLVAAGVAAQVRVGAGLLG
                     "
     gene            complement(365..1195)
                     /locus_tag="PKZ53_17805"
     CDS             complement(365..1195)
                     /locus_tag="PKZ53_17805"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNJ42348.1"
                     /translation="MSVRVFKQDINHLAWRPPDLSDPKLGKSPYQADGTVQPWRAPTH
                     FSASELSHQSFSVFEKPGLAIVADEFPEEPQPDLGMVQAQATAILEAARAQADQLVAV
                     ARSRAAEIEATAREQALLRGREDGLREGRARMQEELAAAAAELRGKMAQSLEDLAILP
                     QLMAEQSESDLVQLAIEIAKKIVHREVTVDREIVLSLVRVALSRINRRSVARIHLNPD
                     EYQFVLTHREKLGTGYALELVEDPTIDPGGCVVETDFGDIDARISQQFAEIERGFFGT
                     "
     gene            complement(1185..>2008)
                     /gene="fliG"
                     /locus_tag="PKZ53_17810"
     CDS             complement(1185..>2008)
                     /gene="fliG"
                     /locus_tag="PKZ53_17810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNJ42349.1"
                     /translation="LNRAQDYVAQGGLDFAKRLLIKSLGPDNARRILDRITQSIESSN
                     RFQSLRKFDPQQLSKLLQNEHPQTIALVLAHLDASQAAETVSCLPESTQAELIMRMAR
                     LQSVSHDVIRRLSVVFEQKLKNMGDFSQQSVGGIRAVAGLCNRLDREVARKMLEEIEL
                     SDPNLALSIRNLMVTFDDLLLVEDMGIREILQRVDKKTIALALKNAIPEIQARFFSNM
                     SVRAVEMMKEDMEFMGQVKLKDVSAAQREVVNTLRELDEKGIISLSGSGGEVYVG"
BASE COUNT          460 a          543 c          541 g          464 t
ORIGIN      
        1 gtccgagcag cccggcgcca acccgaacct gtgccgccac gcccgccgcc accaatcggt
       61 caccagggcg aatctgaggc atttgcccaa gcggaagcaa caggatggtt gaatcacgaa
      121 acccgacaac ttcaagcagc gactctgggg ctgaagggtg gtttgattga tgatcgaggt
      181 aacacaggtc cccaaccgaa acaaaggggc ctttggattc aaccaccagg ccaactgccc
      241 gggtaatcgt ccccagagat cggattgggt caatagattc aagtcgttgc aaatgattgg
      301 tcagcataat tataacgata aacccaattt ctgaacccga tgacttcagc cctggctttc
      361 caggttaggt gccaaaaaac ccacgttcga tttcagcaaa ttgctgactg atgcgggcgt
      421 caatgtctcc gaaatcagtt tcaaccacac atcccccagg atcaatcgtg gggtcttcga
      481 cgagttcgag ggcatagccg gtgcctaatt tttcgcgatg ggtgagcaca aactggtatt
      541 catccggatt aaggtggatc cgggcaactg atcggcgatt gattcgggac agggccaccc
      601 ggaccaggga caggacgatt tcgcggtcaa cggttacttc gcgatggaca atctttttgg
      661 caatttcaat cgccagttgg accagatctg attctgactg ttcagccatc agttgcggga
      721 ggatcgcgag gtcttcgagc gattgagcca ttttgccgcg taattcggca gcagcagcgg
      781 ccagttcctc ctgcatccgg gcgcggcctt cgcgtaagcc atcttcccgg ccccgaagga
      841 gtgcctgttc gcgggcggtg gcttcaattt cagccgcacg acttcgggcc acggcaacca
      901 gttgatctgc ctgggcccgg gccgcctcta aaatggcagt ggcttgagcc tgaaccattc
      961 ccaggtctgg ttggggttcc tcggggaact cgtcagccac gatagccagg ccaggttttt
     1021 caaagactga aaaactctga tgtgacaact cgctggctga gaagtgagtg ggggctcgcc
     1081 agggttgtac cgttccatct gcctgatagg gagatttccc cagcttggga tcagataaat
     1141 ctggtggtcg ccaggcaagg tgattgatgt cctgtttaaa taccctaacc gacatagact
     1201 tcacctccag agccagacag gctgataatg cctttttcat ccagctcgcg gagcgtattg
     1261 acgacttccc gttgagcggc tgaaacatcc ttgagtttga cctgtcccat aaattccata
     1321 tcttccttca tcatctcgac tgcccgaacc gacatgttag agaagaaccg ggcctggatt
     1381 tcaggaattg cattcttgag cgccagtgca atggttttct tgtcaacgcg ctgcaagatt
     1441 tcgcgaatcc ccatatcttc aaccagcagc aggtcatcaa aggtcaccat cagattgcga
     1501 atcgacagcg ccagattggg atctgacaac tcgatttctt ccaacatttt gcgtgccact
     1561 tcgcgatcca gccggttgca taaaccggcc acggcccgaa tgccgccaac cgattgctgg
     1621 ctgaaatcac ccatattttt gagcttttgc tcaaagacaa ctgaaagccg ccgaatgaca
     1681 tcgtgcgaga ctgactggag tcgtgccatg cgcataatca gttccgcctg ggttgattcc
     1741 ggcaaacaac tgacggtttc ggctgcctgt gaggcatcca ggtgcgccag gaccagggcg
     1801 atggtttgtg ggtgctcgtt ttgcaacagc ttggacaatt gttgcgggtc aaattttctg
     1861 agggattgaa accggttgga tgattcgatg gattgggtaa tccggtccaa aatccgccgg
     1921 gcgttgtctg gtccgagcga tttaatcaag agacgtttgg caaaatccaa accgccctgg
     1981 gccacataat cctgcgcccg gttgaggg
//
LOCUS       DAOQDO010001258         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55621_length_2007_cov_3.442111, whole genome shotgun sequence.
ACCESSION   DAOQDO010001258 DAOQDO010000000
VERSION     DAOQDO010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55621_length_2007_cov_3.442111"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>2007
                     /locus_tag="PKZ53_17815"
     CDS             2..>2007
                     /locus_tag="PKZ53_17815"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNJ42350.1"
                     /translation="MEAVNRETIKWCQVFFDEGQATLPMPGRHLGFYRSWLNLAPFDD
                     QLHGSDSKKVTWLNHLPESPEMAIEHCLNWLKIPGEQQPQFLTLLLTTLPGWASYVKY
                     RTAWNPNEKSHGHPVSQAEYLAVRLAVTCLLWPQAARLLAWYQVAKANAPSVAPQLNN
                     IEVAEKQYRLPLLEALSQQSPLVTRTTAKLPADAQLVFCIDVRSEPFRRAIEAQGQYE
                     TLGFAGFFGLPIQIQNETTGETYASCPVLLTPRHTVKEAIACSSETQGQDVSGKEKLS
                     LIKRFYQGLKYSFTTPFALVETLGLWSGAWMLLRTLAPHQAVGLKKRAIESIRPGLPV
                     IPCLNATETATGIPLADQCAYAEGALRMMGLTSNFAPLVVFCGHGSTTENNAYATALD
                     CGACGGNHGGGNAKILAAILNQQRVRAHLAERGISIPNTTWFFGAEHNTTTDDVTLDL
                     PQISDTVILEKLNQLTQDLQSAREVNSRWRCLQMGTTGGDQASARHTVTRSTDWAQTR
                     PEWGLARNAAFIVAPRQLTRHINLEGRSFLHSYDWQQDPQGSSLTTILTAPMVVAQWI
                     NTQYLFSTLDNVAFGSGSKITHNITGKLGIMQGNGSDLMHGLPLQSIYRTDTEAYHQP
                     LRLMTVVYAPRWFIDGIIERQAILRKLFGNGWVTLACIEPEDQQP"
BASE COUNT          468 a          550 c          534 g          455 t
ORIGIN      
        1 catggaggcc gtgaatcggg aaaccatcaa atggtgccag gtattttttg acgaaggtca
       61 ggccactctt cctatgccgg gtcgccacct tgggttttac cgctcctggc tcaacctggc
      121 gccatttgat gaccagcttc atgggtctga ttcaaagaaa gtgacctggc ttaaccacct
      181 gccagagtca cccgaaatgg caattgaaca ttgcctcaac tggctcaaaa ttccaggcga
      241 gcagcagcca cagtttttga cgctcctgtt gaccaccctg ccaggctggg caagttacgt
      301 aaaatatcga accgcgtgga atccaaatga aaaaagccac ggtcatcccg tgagccaggc
      361 tgagtatctg gctgtccggc ttgctgttac ctgtttgctc tggcctcaag ccgccaggtt
      421 gctggcctgg tatcaggttg ccaaagccaa tgcgccatca gtggcaccgc aactcaacaa
      481 cattgaagtg gctgaaaaac agtatcggct tccattgctc gaagccttgt cgcaacagtc
      541 accgctggtc acccggacaa ccgcaaagct tccagccgat gcccagcttg tcttctgtat
      601 tgatgtgcgt tcagagccgt ttcggcgggc tatcgaagct cagggccagt atgaaacgct
      661 ggggtttgcc ggtttctttg gcttgccaat tcaaattcaa aatgagacaa ccggcgaaac
      721 ctatgcctcc tgtccagttt tgttgacgcc caggcacacg gtgaaagaag ccattgcctg
      781 ctcatctgag acacagggcc aggatgtgag cggaaaggaa aaactctctc tgatcaagcg
      841 cttttatcag ggattgaaat atagctttac cacaccgttt gcgctggtcg aaacactggg
      901 cctctggagt ggagcgtgga tgttgcttcg cactctggca ccgcatcagg ctgtcggttt
      961 gaaaaaacga gccattgaat ctatccgccc aggattgccg gttattccat gcctgaatgc
     1021 aaccgaaacc gcaactggca tcccgctggc cgatcaatgt gcctatgctg aaggtgcctt
     1081 gcggatgatg gggttgacct ccaattttgc gccactggtt gttttttgcg ggcacggcag
     1141 caccactgaa aataatgcct atgcaaccgc gcttgattgt ggtgcctgtg gtggcaatca
     1201 cggcggtggc aatgccaaaa tcctggcagc catcctcaat caacaacggg ttcgggcgca
     1261 tctggcagaa cgcgggatct caattccaaa taccacctgg ttttttggtg ccgaacacaa
     1321 cacaaccacc gacgacgtga cgctggatct gcctcagatt tcagacacgg tgattctcga
     1381 aaaattgaac cagttgactc aggatttaca gtcagccaga gaagtaaaca gccgctggcg
     1441 gtgtctccag atgggaacca ccggaggtga tcaggccagt gccagacaca cggtcacgcg
     1501 cagcaccgac tgggctcaaa cccggcctga atgggggttg gcgcggaatg cggcatttat
     1561 cgtcgcgcca aggcagttga cccggcatat caatcttgag gggcgttcgt tcctgcattc
     1621 gtatgactgg caacaggatc cgcagggttc gtcgctgacc accattttga cggcgccgat
     1681 ggtggtggcc caatggatca acactcagta tctgttttca acgctggata atgttgcttt
     1741 tggcagcgga agcaagatca cccacaacat caccggcaag ctcgggatta tgcagggcaa
     1801 tggcagcgac ctgatgcatg gcttgccatt gcaatccatc taccgaacgg ataccgaagc
     1861 ctatcaccag ccactccggt tgatgactgt cgtttatgcg ccgcgctggt ttattgatgg
     1921 aattattgag cgccaggcga tactccggaa actctttggc aatggctggg tcacgctcgc
     1981 ctgtatcgaa cccgaagacc agcagcc
//
LOCUS       DAOQDO010001259         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55650_length_2007_cov_2.388320, whole genome shotgun sequence.
ACCESSION   DAOQDO010001259 DAOQDO010000000
VERSION     DAOQDO010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55650_length_2007_cov_2.388320"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKZ53_17820"
     CDS             <1..486
                     /locus_tag="PKZ53_17820"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNJ42351.1"
                     /translation="LLPKTPEREQHMMNGIQHTFWPGRTEWYEGTPPMLLDGAHNLES
                     MAALAECLATGVKNRPITLVFAAMRDKPAEDLLKLLAPHVAKIVCVPVSGNPRTYSPD
                     ELAEAARKYWREYSIYTDVCVASGLQRAHGLTPPTGLVLVTGSLYLVGEVMAQQAEGQ
                     G"
     gene            complement(631..1179)
                     /locus_tag="PKZ53_17825"
     CDS             complement(631..1179)
                     /locus_tag="PKZ53_17825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ42352.1"
                     /translation="MPHSASSKLLILDVDETLIFGTEHQLNRPPDFQVFGYSIYRRPG
                     VDEFLTFCFDRFQVAIWSSSSEDYVQAVAAHLIPADHQLQFIWSRKQCTLSYDYDLRD
                     HFWTKKLVKAKRKGYSLEQMIVVDDSPEKHVHNYGNLVKIKAFHGELDDDELSHLMRY
                     LEHLDQFPNIRAIEKRWWRNSF"
     gene            complement(1182..1721)
                     /locus_tag="PKZ53_17830"
     CDS             complement(1182..1721)
                     /locus_tag="PKZ53_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42353.1"
                     /translation="MTFELSQSRINLETPQAQALFDALQGVYGNGAVILCCLKSTEIS
                     SLMNNFWLSVPELVISQFLKSQTVKQAVPELKVDDELMLPALHPLNSYELEGSLTQIL
                     INGGAYTQWQSSEDEARSLTRNFLNLVAAEKYRSHVSGIRFYSAWTDWFYDVAWDSTF
                     VIYNAATQKWWLLCTTDTD"
     gene            complement(1734..>2007)
                     /locus_tag="PKZ53_17835"
     CDS             complement(1734..>2007)
                     /locus_tag="PKZ53_17835"
                     /inference="COORDINATES: protein motif:HMM:NF015114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42354.1"
                     /translation="HDEDERICSSAASALGEFGSEKAIESLLTFAHQNMSRGTYSIVS
                     ALGEILHPRSVEALQSFLNDDDPVVRLHTGHALDRLKQRLSTDQSD"
BASE COUNT          531 a          474 c          494 g          508 t
ORIGIN      
        1 cttctgccaa aaacccctga acgtgaacaa catatgatga atggcatcca gcatacattc
       61 tggccaggcc gaactgaatg gtatgaaggc acgccaccaa tgttactgga tggagcccac
      121 aacctggaaa gtatggctgc cctggctgaa tgcctcgcca ccggagtcaa aaaccgtccc
      181 attacgctgg tctttgccgc gatgcgtgat aaacccgccg aagatcttct gaaattgctg
      241 gcgccgcacg tggccaaaat cgtctgtgtg ccagtgagcg gcaatccccg cacctactca
      301 ccggacgaac tggccgaagc tgcccgaaaa tactggcgcg agtattcaat ttatactgac
      361 gtttgtgttg cttcggggtt gcagcgcgcc catggactga ccccgccgac aggactggtc
      421 ctggtcaccg ggtcactcta tctggtcggc gaagttatgg cccagcaagc tgaaggacaa
      481 gggtgaaata ctattgggct gagggctgag ggctgaggga aatccaaatt gtttgatgat
      541 tcgctttctc catttttacc aatgggaaag cagtcaagcg attgaaaata ataccggttt
      601 gtaatgaaat aaacgtgaat ctttgtttgt tcaaaaggaa ttccgccacc agcgtttctc
      661 gatggctctg atgtttggaa actggtccaa atgctctaaa tagcgcatca aatgtgataa
      721 ttcatcatca tccagttcgc cgtgaaatgc ttttattttc acgagattgc catagttatg
      781 cacgtgtttt tcagggctgt catcaacaac aatcatttgc tccaggctgt atcctttgcg
      841 tttggctttc accagttttt tggtccagaa atggtcgcga aggtcatagt cataactcaa
      901 ggtacactgc ttgcgcgacc agatgaattg aagctggtga tctgctggaa tcaaatgagc
      961 cgccactgcc tggacataat cttcgcttga agacgaccag atcgcgacct ggaaacggtc
     1021 aaagcaaaat gtcaggaact cgtccacacc ggggcgcctg taaatggaat acccaaaaac
     1081 ctgaaagtca ggtgggcgat tgagttgatg ttccgtccca aagatcagcg tttcatcaac
     1141 atccaggatc agaagttttg aagaagcaga atgtggcatg attaatcggt gtccgtggta
     1201 caaagtagcc accatttttg ggttgccgcg ttgtaaataa caaatgtcga atcccaggcc
     1261 acatcgtaaa accaatctgt ccaggctgag taaaaccgaa tgccgcttac gtgggagcga
     1321 tacttttcag ccgccaccag attaagaaaa ttgcgggtca gggaacgggc ttcatcttca
     1381 gaagactgcc attgggtgta tgccccgcca ttgatgagga tttgggtcag gctgccttcc
     1441 aattcgtagg agttcaaggg atggagcgcc ggaagcatca gttcgtcatc aactttcaat
     1501 tcgggaactg cttgctttac ggtttgggat ttcaaaaatt gagagatgac gagttctggt
     1561 acagagagcc agaagttatt cattaaacta ctgatttcag tggattttag gcagcacaga
     1621 atcaccgccc cgttaccgta tactccctgg agtgcatcaa acaacgcctg agcttgaggt
     1681 gtttcaaggt tgattcttga ttgactgagt tcaaaagtca taacaattta cttctaatca
     1741 ctttgatcag tactgagacg ctgttttaac cgatccagcg catgcccggt gtgcaggcgt
     1801 acaactggat catcatcatt caaaaatgat tgtaatgctt caactgagcg cgggtgaaga
     1861 atctctccca gagctgaaac tattgagtag gtaccacgtg acatattttg gtgagcgaag
     1921 gtgagcaggg actcaatcgc tttttcactg ccaaattccc ccagggcaga ggccgcagag
     1981 ctacaaatcc gctcatcttc atcatgt
//
LOCUS       DAOQDO010001260         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55742_length_2005_cov_3.246667, whole genome shotgun sequence.
ACCESSION   DAOQDO010001260 DAOQDO010000000
VERSION     DAOQDO010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55742_length_2005_cov_3.246667"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1125)
                     /locus_tag="PKZ53_17840"
     CDS             complement(133..1125)
                     /locus_tag="PKZ53_17840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNJ42355.1"
                     /translation="MTSRLLSIIERFSQQTIVVVGDLIADEFVFGEIARVSREAPVLI
                     LRHERTETMPGGAGNTASNIASLGGNAASIGVVGRDRAGRMLTLSLRSRGINTSGVLS
                     VAGYVTPTKTRILAGSAHAPRQQVIRVDREENGPLPEPARGWVLARLEAQLRQATGVI
                     LSDYNYGLIDSDCIALLQNWQTHHPGIPIVVDSRFRLHQFTGFTSATPNEAEAEDLCG
                     QRFTTSELARVQVEQLRAQLNLKALLLTRGPEGMILVEEGRPAVFEEAIGSKEPVDVT
                     GAGDTVIATYTTALAAGATFPEAMHLANCAAGCVVMKRGTATMSQAELVEKLRE"
     gene            complement(1273..1941)
                     /locus_tag="PKZ53_17845"
     CDS             complement(1273..1941)
                     /locus_tag="PKZ53_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ42356.1"
                     /translation="MSSAVTPVVHNRAVFFDRDGTLSEEVGYVNHISRFQLLPTTGRA
                     IRKLNDAGWRAIVVTNQAGVARGYFEESLIGQVHTTLRAELAKDGAFLDAIYYCPHHP
                     SAGEPPYRQNCNCRKPKTGMLEQAVNTFKVDLSRSYVVGDKYSDVELAHRVGAKGILV
                     LTGYGRGEYEYQRQTWPRDPDFIAEHVLEAVEWILATNNVQLGTTTDRRNTPRSPFRG
                     FPHL"
BASE COUNT          525 a          539 c          478 g          463 t
ORIGIN      
        1 cactcgacag tgccattgtc caggtccagg ttgccgaacg caccaaacgc taactaaata
       61 gtcagtacca cctgcgtcag cgggtgggga tttattttat tcccacccgc tgacgcaggt
      121 ggtactgacg cttcattccc tcagcttttc cactagctcg gcctgactca tcgtcgcggt
      181 cccccgcttc atcacgacac atccagccgc acaatttgct aaatgcatgg cttcaggaaa
      241 cgtagctcct gccgccagag ccgtggtgta ggtagcaatc accgtgtcac cagccccagt
      301 cacatcaact ggctccttgc tcccgattgc ctcttcaaaa accgccggac gaccttcttc
      361 cactaaaatc attccttctg gaccacgggt taacagaagt gccttcagat ttaactgagc
      421 acgtaattgc tcaacctgca ctctggccag ttcactggtg gtaaatcgtt gaccacacag
      481 gtcttcagcc tcggcttcat ttggtgttgc cgaagtaaag ccagtgaact gatgcaaccg
      541 aaaccgtgaa tccaccacaa ttggaatccc agggtgatgg gtttgccagt tttgcaaaag
      601 agcaatgcag tccgagtcaa tcaatccata attgtaatcc gacaaaatga cacccgttgc
      661 ctgtctgagt tgagcttcca gtcgggcaag cacccatcca cgggcaggct caggcaatgg
      721 cccattctcc tcgcgatcca cccgaatgac ttgctggcga ggggcatgag cagagccagc
      781 caaaattcgg gttttggttg gtgtgacata tcccgccaca gataacaccc cactggtatt
      841 gattccccgt gaccgaaggc tcagtgtcag catccggcca gctcgatcac gcccgacaac
      901 cccaattgat gcagcatttc cgcccaatga ggcaatattt gaagccgtat ttcccgcccc
      961 gccgggcatg gtttcagttc gttcgtggcg cagaatcaag actggagctt cacgcgacac
     1021 acgcgcaatc tccccaaaaa caaactcatc agcgattaaa tcaccaacga ccacaatggt
     1081 ttgctgtgaa aatcgctcaa taatcgaaag caggcgggaa gtcataattg aatgaagaat
     1141 tgagaatgaa gaattgagaa tgaagaattg agaatttgtc tggtacctgg tgactggtac
     1201 ccgatactta gtgcttagaa ctcaatattt tccaagaccc aggcactgtc ttcattcttc
     1261 attcttcatt tcctacagat gaggaaagcc ccgaaatggg gagcggggtg tgttgcgacg
     1321 gtcagtggtg gtgccaagct ggacattatt ggttgccaaa atccattcga ccgcttcgag
     1381 gacatgctca gcaataaaat cagggtcacg tggccaggtt tgacgttgat attcatactc
     1441 accgcgacca tacccggtta gtactaaaat tcctttggca ccaacacgat gagccagttc
     1501 aacatcgctg tatttatcac cgacgacata tgagcggctc aggtcaactt taaaggtatt
     1561 gacggcctgt tcaagcatcc cggtctttgg tttccggcaa ttgcagtttt gacggtaagg
     1621 aggttccccg gctgatggat gatgtgggca ataataaata gcatccagaa aggcgccgtc
     1681 tttggccaat tcagcccgga gcgtggtatg cacctgccca atcaatgatt cttcaaaata
     1741 gccgcgtgcc acacccgcct gattggtcac cacaattgcg cgccagccag catcattcag
     1801 cttgcgaatt gcccgtccgg tggtcggcaa gagctgaaaa cgagagatgt gattgacata
     1861 gccgacctct tcgctgagcg taccatcacg gtcaaagaaa acggctcgat tgtggacaac
     1921 cggagtcaca gcagacgaca taaaacctct ggaatgaaga attgagaatg aagaatgaag
     1981 aattgagaat gaagaattga gaatg
//
LOCUS       DAOQDO010001261         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55793_length_2004_cov_3.363776, whole genome shotgun sequence.
ACCESSION   DAOQDO010001261 DAOQDO010000000
VERSION     DAOQDO010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55793_length_2004_cov_3.363776"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKZ53_17850"
     CDS             complement(<1..722)
                     /locus_tag="PKZ53_17850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ42357.1"
                     /translation="MSNRYSAIDSDDLLNRNDERRYQLHAIMALVVLTLVIYVGSAWA
                     PALQDDADAAHAQAAREIVERNDWTTLHINGIRYLEKAPLMYWMVAASYKVFGFTEFG
                     TRFPLALGTLALVLAVYQFGQWMGGVRAGFYAGLAVCVGLGVYLFTRIMIPEVILSFW
                     ITVSFYAFLRAYFEEVDSPWYYVFYAGMAAAVMTKGLIGIVFPCGALALFVLVTGGIT
                     RIWSMRPVTGILLFLLLASPWH"
     gene            959..>2004
                     /locus_tag="PKZ53_17855"
     CDS             959..>2004
                     /locus_tag="PKZ53_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42358.1"
                     /translation="MAVLIGYGIVGSTRESFLGESLIASAHPVTHNRFLQEKSALTGS
                     PTPEEQVSETLRASRHVLKLENGKLTGPGADLLIRDARDSQFVFLGEEHFIAEVPRFA
                     QALWSELTPAGFEHLVIEVGPYTTPVLEAIVKKPGYADQLKAIEARSGLAIPFYTLRE
                     EADFVAAVVQSSKSKIPVLWGLDQENAFAVPDLLERVAALANDEPAKQVAKRVLSEAQ
                     TANTKAITERNPGLLAMMQMKPETFGELRTALASKSNAPALALVNGIEESKAIYDLYF
                     QGKGYESNLRREQYLKTNFIQAFREAQRQTGHSPKAMLKFGFNHAYRGRNAVEVHGIG
                     NFIAEKAAAEGKSSL"
BASE COUNT          521 a          548 c          508 g          427 t
ORIGIN      
        1 atatgccagg gtgaggccag cagcaaaaac aggagaattc cggtcactgg gcgcatcgac
       61 cagatgcggg taattccgcc cgtcaccagc acaaacaacg cgagtgcccc acagggaaaa
      121 acaatcccga tcaaaccttt ggtcatcacg gcagcggcca ttccggcata aaacacgtaa
      181 taccagggtg aatcaacctc ttcaaaatag gcccgtaaaa aggcatagaa tgagaccgta
      241 atccagaagc ttaaaatgac ctccgggatc ataatccggg taaacagata aacgcccagc
      301 ccgacgcaga cagccaatcc agcgtaaaat ccggcacgca ccccgcccat ccactgacca
      361 aactgataca ccgccagcac cagcgccagc gtgccgagcg ccagcgggaa gcgtgtccca
      421 aactcagtaa atccaaagac tttatacgaa gcagccacca tccagtacat cagcggcgct
      481 ttttcaagat agcgaatacc gttgatatgg agcgtggtcc agtcgttgcg ctcgacaatt
      541 tcacgggcag cctgggcatg tgcggcatcg gcatcatctt gcagggccgg tgcccaggcg
      601 ctgccgacat aaatcaccag cgttaacacc accagcgcca tgatggcgtg caactggtag
      661 cggcgttcgt catttcggtt gagcaggtca tcagaatcaa ttgcagaata tcggtttgac
      721 atgtgagcga tgaattgaag ggaactgaga gtgttttgca ggttagaaaa gccaaatttc
      781 cagtaggctt acacattttt gcaaaaaaag gtagtatcag cccgcgcagt tcccaaagat
      841 tctcacccaa caactgaatc aggatttgga ggtttcaatg cttcacaagc ggcaggttgt
      901 gcctgcgagc gtctctgtct ggcctcgtct tcgctctgtt ctttgtcttc tcggggtttt
      961 ggcagtgctc atcggatacg ggattgttgg ctcaacccgt gagtctttcc tcggcgagtc
     1021 gctcattgcc agtgcccacc cggttactca caaccgattt cttcaagaaa aatcagctct
     1081 gactgggtct ccaacccctg aagaacaggt ttcggaaacg ctccgtgcca gccgtcacgt
     1141 gttgaaactg gaaaatggaa agctgaccgg tcccggcgct gaccttctga ttcgagacgc
     1201 ccgtgactcg cagtttgttt ttcttggcga agagcatttc attgctgaag ttccacggtt
     1261 tgctcaggca ctctggtcag agttaacacc tgccgggttt gaacatctgg tcatcgaagt
     1321 tggcccctat accacgccag tcctcgaagc aattgtgaaa aaaccgggat atgccgacca
     1381 gttaaaagcg attgaagcca gatccggact ggccattccg ttttatacct tgcgcgagga
     1441 agccgatttt gtggcagcgg tggttcaatc ttcaaaatcg aaaatcccgg tactctgggg
     1501 gctggatcag gaaaacgcct ttgcggttcc tgatttgtta gagcgagttg cggcacttgc
     1561 caacgatgaa ccggcgaaac aggtcgccaa acgagtgctc agcgaagccc aaaccgcaaa
     1621 caccaaagcc atcactgagc gaaatcctgg cttgctggcg atgatgcaaa tgaagccaga
     1681 aacttttggt gaattgcgca ccgcccttgc ctccaaatcc aatgccccgg cgctggcgct
     1741 ggtcaatggc atcgaagagt ctaaagcgat ctatgacctg tattttcagg gcaagggcta
     1801 tgagtctaac ctccgccgcg aacaatattt aaagaccaat ttcatccagg cgtttcgcga
     1861 agcccagcgc cagactggcc attcaccgaa agccatgctc aagtttggct tcaaccacgc
     1921 ctatcgtggg cgcaacgcgg tggaagttca tggcatcggg aattttatag ccgaaaaagc
     1981 cgcagccgaa ggaaagagca gtct
//
LOCUS       DAOQDO010001262         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55804_length_2004_cov_2.874295, whole genome shotgun sequence.
ACCESSION   DAOQDO010001262 DAOQDO010000000
VERSION     DAOQDO010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55804_length_2004_cov_2.874295"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKZ53_17860"
     CDS             complement(<1..287)
                     /locus_tag="PKZ53_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42359.1"
                     /translation="MQDEGQVQFKRGRLKRLVVVLFLLAGSFGLVSIGLKLYIHRTCE
                     GRVYREVTEVPSEDEPRIAIVFGAGVWPNGQPSPVLYDRVATAADLYHAGKA"
     gene            complement(385..1161)
                     /locus_tag="PKZ53_17865"
     CDS             complement(385..1161)
                     /locus_tag="PKZ53_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42360.1"
                     /translation="MHNQEPPPQEILSIVERLERMFAFDRMYKGGQGGIDIRGKYRED
                     MNAFLESTKYLKTSFSQSPQLLEQTFHHEALKKEPLLRKYLSEKIHSWFPQSPSLLAI
                     VNSHSEIQRESPEAVPSASRKGVIDIRAAQLEINEIGKLKAAYSELVAERNELLEEVT
                     QLTKRVEGLTRELSQRTEKEKPTSDTTKNDRMIRSYQNLINTITGIFSVMFMNKKHVS
                     FSEFSAAMQSVIRHIRNHNKTHEDRIDIESLEFLLKENPE"
     gene            1610..>2004
                     /locus_tag="PKZ53_17870"
     CDS             1610..>2004
                     /locus_tag="PKZ53_17870"
                     /inference="COORDINATES: protein motif:HMM:NF014199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ42361.1"
                     /translation="MKIRVLPSITDQRSQLLISFLINDCVSVDAGAVAFHLTGEKLLR
                     VKDLVLTHVHLDHIASFPFIFSEEFTEIREPVRIHASERDITSLKAHIFNNVIWPDFT
                     KIPNQHGQLLAFHPYTTHQPFEVAGLTITP"
BASE COUNT          489 a          479 c          448 g          588 t
ORIGIN      
        1 gctttgccag cgtgatagag gtcagccgcc gtggccactc gatcatacag aaccggcgac
       61 ggctgtccat tgggccagac cccggcccca aaaacgatgg cgatgcgtgg ctcatcttca
      121 gatggaacct ctgtgacctc gcgatagaca cgcccctcgc acgtgcggtg aatatagagt
      181 ttgagtccaa tcgaaaccaa accaaacgaa cctgcaagca aaaaaagaac tacaaccagt
      241 cgtttgagtc gccctcgttt gaattggacc tggccttcat cctgcatagt gtttattcag
      301 gttccgggtt ccgcgcttcc gagttccggg ttttcgataa atccctacct gctcaagcaa
      361 atggattgac tcctcgcttg ctcactactc cgggttctct tttaagagaa actccagcga
      421 ttcgatatca atccggtctt cgtgggtttt attgtgattg cgaatgtgtc ggataaccga
      481 ttgcatggcc gcactgaatt ctgaaaaact gacgtgcttt ttattcataa acatcacgga
      541 gaaaatcccg gtgatggtgt taatcaggtt ttggtacgag cgaatcatgc ggtcattttt
      601 ggtggtatcg ctggtcggtt tttctttttc ggtgcgctga ctcagttcac gggtgagccc
      661 ttcaacacgt ttggtgagct gggtaacttc ttcaagcagt tcattgcgtt cggccaccag
      721 ctcagaataa gccgctttta atttcccaat ttcattgatc tcaagttgtg ccgctcgaat
      781 atcaatgaca cctttgcgcg aagccgacgg aacggcttca ggtgactcgc gctggatttc
      841 actatgcgag ttgacaatag cgagcaatga cgggctctgt ggaaaccagc tatgaatttt
      901 ttcactgaga tatttgcgca gaagcggttc tttcttcagg gcttcatggt gaaacgtctg
      961 ctcaagtaac tgaggggact gtgaaaaact ggttttcagg tatttggttg attccagaaa
     1021 agcattcatg tcctcccgat atttgccacg gatatcaatg ccgccctggc cgcctttgta
     1081 cattcgatca aaggcaaaca tgcgttcgag ccgttcaaca attgaaagta tttcctgggg
     1141 agggggttcc tggttatgca tagcgtcctc tcgtcatcca attcggtttc ttccgcaatt
     1201 ctatttattg ggtctttgtc ttcaaatgtc aacttccgaa acaggatggc ggtcagcaag
     1261 tgctgtggga ggcatggctg attgatcgtc aagagaaaaa tcaaaatctg agtcagcaca
     1321 cgattcttta aagagaaatc attggatttt ctattttttt attgaattaa aataaaatta
     1381 tagttatttt ctattttata ttcgttgatt atctcaccga tggccttaga taactgactt
     1441 ttaggtgaat gagttgactg gagtttcaaa ggtagtgcag acttgaccac accaagcaac
     1501 acacattttt catacatccc agggaaacag ccattttcta gccagatgcc ttttgttgcc
     1561 agaccgtcac tggcaacttc aatccacttt ttatctagac gagcatttta tgaaaatccg
     1621 tgttctgccc agcattacag accagcgcag ccagttgctc atttcatttc ttatcaatga
     1681 ttgtgtatcg gttgatgctg gtgcggtcgc tttccatctc actggggaaa agttgcttcg
     1741 cgtgaaagat ctggttttga cccacgtgca tctggatcac attgcttcat ttccgtttat
     1801 tttttccgaa gaattcactg agatccgcga gccagtccga attcatgcct ctgagcggga
     1861 tattaccagt ttaaaagcgc atatcttcaa caatgtcatt tggcccgatt ttacaaaaat
     1921 cccaaatcaa catggccagt tgctcgcatt tcacccatat accacccatc aaccctttga
     1981 agtggcgggc ctgaccatca cccc
//
LOCUS       DAOQDO010001263         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55835_length_2004_cov_1.818368, whole genome shotgun sequence.
ACCESSION   DAOQDO010001263 DAOQDO010000000
VERSION     DAOQDO010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55835_length_2004_cov_1.818368"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKZ53_17875"
     CDS             complement(<1..797)
                     /locus_tag="PKZ53_17875"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNJ42362.1"
                     /translation="MNLQCSEMPPTLYHPIIEAGQKAEAELDRRKNARTHLIDFTRYT
                     FPGYSADPAHELIAQTLDQVVSGEIKRLMIFAPPQHGKSELASVRLPAFWLAKNPNLP
                     VILTSYGASLAEDKSEQVRTLVESEGFRRLFPKVSTHPESRARSLWKLCEPHRGKLIA
                     AGVGGPITGFGGGLGIIDDPVQNWEAAQSKTQKEKTWHWYRSTFRTRIWEGGAIVLMM
                     TRWSEDDLAGRILDEQAEEWTILRLPAVAEDQTDRDANNETYHLPAG"
     gene            complement(745..1323)
                     /locus_tag="PKZ53_17880"
     CDS             complement(745..1323)
                     /locus_tag="PKZ53_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42363.1"
                     /translation="MAAKKFTEREREEIYVKVSELWKKDWTAKQMAVAVGVSERQVQR
                     YVQKIRAGWQEQVRRNAEEKMAHMLAQQSKVKQEAWEQWERSKQDGQKVLKLLGKEKK
                     DGDGDGDESPGLVVKTETWGQCGDPRYLEKIQKAIDYETELQGLFPPKRIAPTTPKGD
                     ALFSFLSKPIEEMTDDELAMLRNAANALSPNH"
BASE COUNT          446 a          505 c          490 g          563 t
ORIGIN      
        1 attccagcgg gcaaatggta ggtttcgttg ttggcatccc gatctgtttg gtcttcggcg
       61 acggcaggca gccgcaaaat ggtccactcc tcggcttgct catccaagat tcggcctgcc
      121 aaatcgtcct cactccaccg ggtcatcatg aggacaattg ccccaccttc ccaaatacgg
      181 gttctgaatg tggaacggta ccaatgccag gttttttctt tttgagtttt cgattgggcc
      241 gcttcccaat tctgaacggg atcatcaatg atcccaagtc cgcccccaaa tccagtgatt
      301 ggaccaccaa caccagcggc aatgagcttc ccccgatgag gttcgcacag tttccacagg
      361 ctacgtgccc ggctctcagg atgagttgag actttgggga acagccggcg aaacccttcg
      421 gattcaacca gtgtccggac ttgttcgctt ttgtcttcag cgagacttgc cccataactg
      481 gtgagaatca cgggaagatt tggatttttt gccagccaaa aagcagggag tcgcacactg
      541 gccagctcgg atttgccgtg ctggggtggg gcaaaaatca tcaaccgctt gatttcaccc
      601 gacacaacct gatccaaagt ctgggcaatc agttcgtggg ccgggtccgc actgtaacca
      661 gggaaggtat agcgagtgaa atcaatgaga tgggtacggg cgtttttgcg ccggtcaagc
      721 tcagcttcgg ctttttggcc agcttcaatg attgggtgat aaagcgttgg cggcatttcg
      781 gagcattgca agttcatcgt cggtcatttc ctcaatcggt ttactgagaa acgagaacaa
      841 cgcatctccc ttgggtgtgg ttggggcaat cctttttggc gggaataacc cttggagttc
      901 agtctcgtaa tcaatggctt tctgaatttt ctcaaggtag cgcgggtcac cgcattgtcc
      961 ccaggtttca gttttcacga ccaggccggg tgattcatca ccatcaccat caccgtcctt
     1021 cttttccttc cccagaagtt tcagtacttt ctggccgtcc tgcttggagc gttcccattg
     1081 ctcccaggct tcctgtttca ccttgctttg ctgggcaagc atgtgcgcca ttttctcctc
     1141 agcattgcgc ctgacttgct cctgccatcc tgcccgaatt ttttgcacat atcgttgaac
     1201 ttggcgctcg gaaactccta cagcaaccgc catttgcttg gcagtccagt cttttttcca
     1261 tagctccgag acttttacat aaatctcttc gcgttctctt tctgtgaatt tttttgcagc
     1321 cataacgaca cacccatgaa acgacgcaga agcgtaacac tcgaatggtg gttgtttccg
     1381 caagagagaa gcagcaggga atttacttga gggagtagag gaaaggatga gcagcgcctg
     1441 actgggattg atgacggcag gtgatcagct tcgcttgaag aagtgaatca agggtggacc
     1501 gaactatcca ctctttgaac ccggttgatt gctggatgtc ttctttggtg caatatccct
     1561 tttggattgc caatagaacc aacccttgac ggagcttcag attcataact tgcccggaaa
     1621 aatttctgca atttgatagg actctatcaa aatgagaaca tcgttttcgc cacctgtaaa
     1681 aattgatttc aggtattccg agagcttgca ttgtcgcccg gatcgacaac ccggcttgca
     1741 agcaatcagt aatttgggct gaaatttctg gtgtgagctt ggttttcctt cctggcataa
     1801 acgaatctac aatgtgtgac acttttgtga cggactggtg tatatcgtta ttagcgacga
     1861 tgacttttat tggtttcgag gacggctgca accgttgatg gttatttgtt atttacgatt
     1921 aagtatgtgc attaccaagt ttttaacgca ctcttaatca gcgggtcgca ggttcgagtc
     1981 ctgctcggct catcactcaa taaa
//
LOCUS       DAOQDO010001264         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55960_length_2001_cov_3.091470, whole genome shotgun sequence.
ACCESSION   DAOQDO010001264 DAOQDO010000000
VERSION     DAOQDO010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55960_length_2001_cov_3.091470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2001
                     /locus_tag="PKZ53_17885"
     CDS             <1..>2001
                     /locus_tag="PKZ53_17885"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNJ42364.1"
                     /translation="QTVQTQTVKGTTRQKVAFLFGATQADVARSSRQSLAQSSIYTQA
                     FTHCVQLGQQAALTSDDADLLTFADSIALAEVWKAFGVLPGAVLGQELGELAAAVVAG
                     CCSIETAIAVLAARRRALDQLPTGGMTIQVATSGAHVTTFLQSGTSGVTIAGVCSPEH
                     TLVAGEHSAIAAFMTRLAAAGISSQIVHPRLALHSPLVEPVLAGFEQSIQTLSFEEPQ
                     IPMISGVTGRQLDGVPDAMYWRQQLRQMLKLTPAFDTLSELGFETFIVIEPTCQLLTI
                     GKQSLGETFGTWLPGAGQAGSDDWRTLLQGLTYLYLNGTDIEWSAAYRDLPRRRIGLP
                     TYPFDRHRYWVEPPVIAELQTVAAPPALTFEALAASIAEVSIRTLAENGESPTEADVN
                     LFDAQGRLVVSLPGRRISQTIKTDSLPDSSWFYELRWKEISHPATVSQSETPGSWIIF
                     THRNKFGRALTALLEAAGETCTVIKPGAAYDNTGEDEIEINRERAEDYDQLFAEALKP
                     GCRGVLFLWGLDEKGMEAGGQGIDALKANLRLVQALARHTTKAARTGNRLTPKLWVIT
                     RGAQPVAGEHSTSGMVQAALWGFGRVTALEHPSLWGGLIDLAPTPSLEEATLALRAIL
                     NPDAEKETGLRGGKRFAPRLVRSPYDETQTSQMTCRADATYLV"
BASE COUNT          417 a          609 c          546 g          429 t
ORIGIN      
        1 caaacggttc aaacccaaac ggtaaaaggc acgacccgcc agaaagttgc tttcctgttt
       61 ggggccaccc aggctgatgt tgcccgcagt tcccgccagt cactcgccca gagttcgatt
      121 tacacccagg catttaccca ttgcgtgcaa cttggtcagc aggccgcact gaccagtgat
      181 gatgctgatc tgctcacgtt tgccgattcg attgccctgg ccgaagtctg gaaagcgttt
      241 ggtgtactgc caggtgccgt tttaggccag gaacttggcg aacttgctgc cgccgtcgtc
      301 gctggctgct gttcgattga aacggcaatt gcggttctgg cagcccgacg gcgtgccctg
      361 gaccagcttc caactggcgg aatgacgatc caggtcgcca ccagcggcgc tcatgtgacc
      421 accttcctgc aaagcggcac ttccggagtc accatcgctg gcgtgtgcag ccccgaacat
      481 accctcgtgg ctggcgaaca cagcgccatt gccgccttta tgacccgact ggccgccgcc
      541 gggatttcca gccagattgt tcacccacgg ctggcgctgc attcaccact ggtagagccg
      601 gttctggccg gttttgagca gtcaattcag actctttcgt ttgaagaacc ccagattccg
      661 atgatctctg gggtgactgg tcggcaactg gatggcgtgc cagatgccat gtactggcgg
      721 caacaactcc gccagatgct caagcttacc ccggcctttg acacactttc cgaactgggc
      781 tttgaaacct ttatcgtgat tgagccgacc tgccagttac tcaccattgg caagcaatcg
      841 ctgggtgaaa cgtttggcac ctggctgcct ggtgccggtc aggctggttc agatgactgg
      901 cgtacgctcc tgcaaggtct cacctatctt tatttgaatg gaacagacat tgagtggagc
      961 gcggcctatc gtgacctgcc ccgccgtcgc attggccttc caacctatcc gtttgaccgg
     1021 catcgctact gggtcgaacc acctgtgatt gctgaactgc aaacggtggc ggcaccgccc
     1081 gcactcactt ttgaagcact ggccgcctca attgccgagg tcagcattcg cactttggct
     1141 gagaacggtg agtcaccaac tgaagccgat gtgaacctgt ttgacgccca gggcaggctc
     1201 gtggtgtcac ttccaggtcg ccgaatttca caaacgatca agaccgattc gctgcctgat
     1261 tcaagctggt tttatgaact ccggtggaaa gaaatttcac atccagccac agtttcgcaa
     1321 tctgagacac ctggaagctg gatcattttt acccaccgca acaaatttgg tcgggcttta
     1381 acggctctgc tcgaagccgc cggcgaaacc tgcaccgtga ttaaacctgg tgcggcctat
     1441 gacaacacgg gcgaagatga aattgaaatc aaccgtgaac gcgccgaaga ctacgaccag
     1501 ctttttgccg aggcgctcaa acccggctgc cgtggtgtcc tctttctatg gggacttgat
     1561 gaaaaaggca tggaagctgg cggccagggc attgatgccc tgaaagccaa cctgcgactg
     1621 gtgcaggccc tggcccggca caccaccaaa gcggcccgca ccggaaatcg cctgacgccc
     1681 aaactgtggg tgattactcg cggcgcccaa cctgtggcag gtgaacattc aacttcaggc
     1741 atggttcagg ctgccctgtg gggctttggc cgggtgacgg ccctggaaca tccatcgctc
     1801 tggggcggat tgattgatct ggcgccaaca ccgtccctcg aagaagccac gctggctctg
     1861 cgcgcgatcc tcaatccgga tgccgaaaaa gaaaccgggc tccgaggtgg aaagcgattt
     1921 gccccacgcc tggttcgaag cccctatgac gaaacccaaa ccagtcagat gacctgccgc
     1981 gccgatgcca cctatctcgt c
//
LOCUS       DAOQDO010001265         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_55968_length_2001_cov_2.695786, whole genome shotgun sequence.
ACCESSION   DAOQDO010001265 DAOQDO010000000
VERSION     DAOQDO010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55968_length_2001_cov_2.695786"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKZ53_17890"
     CDS             complement(<1..211)
                     /locus_tag="PKZ53_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42365.1"
                     /translation="MRVFALGDPHLSFARPKPMHIFGEHWRDHARKIADAWAALALQP
                     GENGVAGGDDDVLILAGDLSWAMKAE"
     gene            647..>2001
                     /locus_tag="PKZ53_17895"
     CDS             647..>2001
                     /locus_tag="PKZ53_17895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ42366.1"
                     /translation="MSFPRVVPSDAYILRTATVALVLCDLIVATASFLVAFYLRHNEA
                     VFYRPLGAWLPTDITWGFRPYFALLAFIPVVRILTLRYYGLYQLRGEYAFFEDATHLV
                     KAVTMSSLFVVVIVFFFRGGFAFQTFSYSRLVFVYDWALALGGYALVRILLRSLQNIL
                     RHRSLNLIPTLVVGTGQSSQVCIAEISEEPRLGYYVVGALATSSSTPEELEKGEVQGV
                     PILGQFDDLPALAKQSGVTEILITDSTLPSRDIFQSMMRVGRKHHLTFRVVPNLFNCL
                     PRKTEIDQIGSLPMIKLFEDPLAGPNRILKRTIDIVGASVGIILLSPFWAVIAFLIKR
                     ESPGPALYKQERVGMDGRVFQMYKFRSMRVDADDSIHREVMRQNITNAQSANQGDDEQ
                     PIFGKIANDPRVTRVGRFIRKWSVDELPELILVLLGRMSLVGPRPHLPEEVESYSPSQ
                     RR"
BASE COUNT          439 a          540 c          487 g          535 t
ORIGIN      
        1 cttcagcctt catcgcccac gaaagatcac cagctaaaat gagaacatca tcgtcaccac
       61 ctgccactcc attttcaccg ggttgaaggg caagcgccgc ccaggcatcg gcaattttac
      121 gggcatggtc tctccagtgc tcaccaaaga tgtgcattgg tttggggcgg gcaaacgaaa
      181 gatgaggatc accgagagca aaaacacgca ttcaacttta gggttcaggg ttcagggttt
      241 ggcactcttt agaaatttgg gaaatcccaa aacctgaatg aaatgcaact gggacgaatg
      301 gctatgagat tgacatcctc cccgcaataa atcgcggggt tttctgccgg ttatctataa
      361 aacttaacac actgcgccaa aaactcgaaa cttctctgca agtttggtat gctcgctggc
      421 cgttcagagc agagcgcaaa tttccgaagt gacagcacac tttgtcaaca tcctcatttc
      481 aaataccaaa aatgaacaat gaattatgaa agatggctag tgcatctcat tgaattataa
      541 ttattttgtc tcctcttcat ttttcattct tcattttcga ttcttcattc ttcatatgtc
      601 atccaaaggt ccggggtctt cctccgcact ccctcaaaca caagtgttgt cttttccacg
      661 cgtggtccca tctgacgcct atatcctgcg cactgccacg gtggcgctgg ttttatgtga
      721 tttgatcgtg gccacggctt cgtttctggt ggcgttttat ctgcggcaca atgaagcggt
      781 tttttatcgt ccgctcggtg cctggttgcc gacggatatt acctgggggt ttcgtccata
      841 ttttgcactg ctggccttca ttccagtggt tcgcattttg accttgcgct attacggact
      901 ctatcaactg cgcggcgaat atgccttttt tgaagatgcc acccatctgg tcaaagccgt
      961 cacgatgagt tccctgtttg tggtggtgat cgtgtttttc ttccgtggcg ggtttgcctt
     1021 tcagacgttt tcctattccc ggttggtgtt tgtctatgac tgggccctgg cgcttggtgg
     1081 ctatgcgctg gtgcggatcc tcctgcgaag cctgcaaaat atccttcgtc accggtcatt
     1141 gaacctgatt cccacgctgg tggtggggac ggggcagagt tcacaggtat gcattgctga
     1201 aatttccgag gaaccccggc tggggtacta tgttgttggg gctctggcca caagcagttc
     1261 gacaccggaa gaattagaaa agggtgaagt tcagggagtg ccgattttgg gtcagtttga
     1321 tgatctgcca gcgctggcca agcaatccgg agtgactgaa atcctgatta ccgactcaac
     1381 tctcccttcc cgcgatattt tccagtccat gatgcgcgtg ggacgcaaac accatttgac
     1441 ctttcgggtg gtgccaaatt tattcaattg cctgccgcgc aagacggaaa ttgatcaaat
     1501 tggctcactg ccgatgatca aactctttga agacccactg gcgggcccca atcgaatcct
     1561 caaacgcaca attgacattg ttggcgccag tgtcggcatc attctcctgt ctccgttctg
     1621 ggcggtgatt gcttttctaa ttaaacgcga atctcccgga cccgccctct ataaacagga
     1681 gcgcgtcggc atggatggac gtgttttcca gatgtacaaa ttccgatcca tgcgcgttga
     1741 tgccgatgat tcaattcacc gcgaagtcat gcgccaaaac atcaccaatg ctcagtccgc
     1801 caatcaaggc gatgacgagc agcctatctt cggcaaaatt gccaatgacc cccgcgtgac
     1861 tcgcgtcggc cggttcatcc gcaagtggtc cgtcgacgaa ctcccggaac tcatcctcgt
     1921 gctgctcggc cgcatgagcc tcgtcggccc ccgtccacac ctcccggaag aggtcgagag
     1981 ctattcgccg tcgcaacgcc g
//
LOCUS       DAOQDO010001266         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56034_length_2000_cov_2.394859, whole genome shotgun sequence.
ACCESSION   DAOQDO010001266 DAOQDO010000000
VERSION     DAOQDO010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56034_length_2000_cov_2.394859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKZ53_17900"
     CDS             <1..948
                     /locus_tag="PKZ53_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNJ42367.1"
                     /translation="ALIGLYETGIVTSFVSGMPAEALLTPGTQILRTPDVPVKIGNLR
                     DPQTLLAILGFLIMVTLLYRKVRGAIFLGIVVTAVIGVLLGFGKAPTGVVALPFTGEY
                     SLAPLAFKLDIAGVLRLSFLPLLLTLFLMGFLDTLGTLVGVGAAANILDEKGDFPEIE
                     RPMTVDALTCMFSGLIGTSTSGAYIESATGIREGARTGLAALTTAALFAASLFFIPLV
                     SPLQQLRFAYAPALIAVGLLMVGSITKINFDDWTELVPACATIAMMVFTYNIGNGLTA
                     GLVLYPVLKVLTGRAKELNGGSVVLGALCFIYYVFGLPH"
     gene            1154..1621
                     /locus_tag="PKZ53_17905"
     CDS             1154..1621
                     /locus_tag="PKZ53_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42368.1"
                     /translation="MFLKVLKFIQLPLVMIVIFTIGRFWLGLAGVPYAPRGSSTFSIV
                     VLTEISAIYYGAMSGKIAKMSWIETAVVGVMLGLFAQIMIFSATFASYALNLNTYFLH
                     WDALNVKEGTVLTMSEGMMRRAPALAIGSIFTMVFALLGRGVFGFFVTDPNKN"
BASE COUNT          450 a          451 c          545 g          554 t
ORIGIN      
        1 gcgttgattg gtctgtatga aaccggcatt gtcaccagct ttgtgtctgg gatgccggct
       61 gaagcgctgt tgacacctgg aacacagatt ctcagaacac cagatgtgcc agtgaaaatc
      121 ggcaatttgc gtgatccaca aaccctgctg gccattcttg gctttctcat tatggtcacg
      181 ctgctctatc gaaaagttcg aggtgcaatc tttcttggaa ttgtggtcac agcagtgatt
      241 ggggtattgc ttggatttgg aaaagcccca accggagtgg tggcgcttcc cttcacgggt
      301 gaatacagcc tggcgccact ggcgttcaaa ctcgatattg caggtgtgct ccggttaagc
      361 tttctgccat tgttgctgac tttatttctc atggggtttt tggacacgct tggcaccctg
      421 gtcggagttg gggcggcggc aaatatcctt gacgaaaaag gcgatttccc tgaaattgaa
      481 cgcccgatga cggttgatgc actgacgtgt atgttcagcg gcttgatagg aacatcaacg
      541 agcggtgcct atattgaatc ggcaaccggc atccgcgaag gcgcccggac cggactggcc
      601 gccctgacca ccgcggcatt gtttgccgcg tcactcttct ttattccact ggtgtctcca
      661 ctgcaacaat tgcgatttgc ttatgctccg gcattgattg ccgttggctt gctgatggtg
      721 ggatcaatta ccaaaatcaa ctttgatgac tggaccgaac tcgtcccggc ctgtgccacc
      781 attgccatga tggtctttac ctacaacatc gggaatggat taacggcggg gctggtttta
      841 tatccagttt taaaggttct taccggtcgt gcaaaagaac tcaatggcgg ctcagttgtc
      901 ctgggcgcct tgtgttttat ctactatgtg tttgggttac cacactaaaa acaagtgaag
      961 aaaatttgaa tgcagaatga agaatgaaga attgagaatg aagaaaaact ggttcctggt
     1021 gcttggttct tagttcttgg ttcttggtgc ttggttcttg gttcttggtt cttggaaggt
     1081 attggttcca aagcacgaag taccaggcac gagatttctt cctgctcaaa tctttgctca
     1141 aaaagggagt tttatgtttc tgaaagtgtt gaaattcatc cagttgccgc tggtgatgat
     1201 tgtaatcttt acgattggcc ggttttggct cggactcgct ggtgttccat atgccccacg
     1261 aggaagctcc acgttttcaa ttgttgtgct gaccgaaatc agtgcgattt actatggcgc
     1321 gatgtctggg aaaatagcga aaatgtcctg gatcgaaacg gcggtggttg gggtgatgct
     1381 tgggttgttt gcccagatca tgatcttttc ggccaccttt gccagctatg ctctgaattt
     1441 gaatacctac tttcttcact gggacgcgct caatgtgaag gaaggaacgg ttctcaccat
     1501 gtcagaaggt atgatgcgac gggcaccagc cctggcgatt ggttcaattt tcacgatggt
     1561 ttttgccctg cttggacgcg gggtctttgg attttttgta actgacccga ataaaaatta
     1621 gggttgaaaa ccagggcttg gggctgaaga cattgggctg agggcggaag actcgcaagc
     1681 tcggggctga agaaagcggg ttcaagacac taagcccgac atcttcagcc ccaaatcctc
     1741 agcccaatgt cttgaaccct caacccacgt caccttctaa cctttcccag ttagtcttta
     1801 tcattggcaa atggcattgt gaggcaaggc tgaagggggc ggaagggacc tggcaaccat
     1861 actggcaacc tggatcggtc gctgcatacc tggatggcta cgtgatggcc ggtgagagtc
     1921 gaatatatga tgagagaact tggacacatt tgcgaactgg tgcgatatcc cgtcaagtca
     1981 atggcaggga gtgtgacgga
//
LOCUS       DAOQDO010001267         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56119_length_1998_cov_3.403500, whole genome shotgun sequence.
ACCESSION   DAOQDO010001267 DAOQDO010000000
VERSION     DAOQDO010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56119_length_1998_cov_3.403500"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1305
                     /locus_tag="PKZ53_17910"
     CDS             142..1305
                     /locus_tag="PKZ53_17910"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ42369.1"
                     /translation="MRCAQERYLSVIARISSPGNILCAESGYVKPHAQRGKGCTGALQ
                     YKRILMSLPQTAIAYMERQKERVRAMRPKKRIVFPEGTDVTVHERDGRLFAPGIGDNA
                     SGLTGVLTLARCFTQVPLKTKQSILFVGTVGEEGPGNLRGVRYLFQENPYGKQITEFI
                     AYDGPGIERITHQALGSRRYEVKFEGPGGHSWGDYGIVNPVHALGKFIHYMTSYHPPT
                     IPRTTYSISVIEGGTSVNTIPRSARCEVDLRSVSEGELDHLEHHLREAVKQAWEEEER
                     AAALSRTRLKLTVTPMGNRPSGETPTTTRLVRLAQAATMICGCRPLLDCSSTDSNLPI
                     SLSIPAITIGAGGRCNGCHTLDEWYEPTERELSLQRGILLLLGLVGLAEKTSG"
     gene            1709..>1998
                     /locus_tag="PKZ53_17915"
     CDS             1709..>1998
                     /locus_tag="PKZ53_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42370.1"
                     /translation="MTSLLFWSVVEQIGEFMSSVAATVSVRPRLNWVNITVFSLLHLA
                     AFAAFFVRPTWTSLALFVIFYLLTGFGITIGFHRLLAHRGFECSKFLIRLWA"
BASE COUNT          427 a          524 c          530 g          517 t
ORIGIN      
        1 cccggctggc atcggcgaac tcgattcggc tggcgaacgt agtggcctgg gtgcagattg
       61 catcacggcc tgcagtgggt tgagcgctgg cggcgatggc cagtgctccc acgatgactg
      121 cattcgacac gattccacgg attgcgttgc gcacaagaac gctacctttc tgtcatcgcc
      181 cgcatcagca gccctgggaa cattttgtgc gccgagtctg ggtatgtcaa gccgcatgct
      241 caacgcggga agggatgcac gggggcgcta caatacaagc gaatccttat gtctctgccg
      301 cagaccgcca tcgcatacat ggaacgccag aaagagcgtg tccgtgccat gcgtccgaag
      361 aaacgcattg tgttcccgga aggaacggat gtcacggtcc acgaacgaga tggacgtctg
      421 tttgctccgg gtatcggcga caatgccagt ggcctgactg gtgtcctgac cctggcccgg
      481 tgttttactc aagttccact taagaccaaa cagtcaattt tgtttgtggg caccgttggc
      541 gaagaaggcc caggaaattt gcggggtgtc aggtacctgt ttcaggaaaa cccctatggc
      601 aaacaaatta ctgaatttat tgcctatgac gggccgggaa ttgaacgcat tacccatcag
      661 gcactcggtt cgcgacgcta tgaagtcaaa tttgagggac ctggcgggca ctcgtggggc
      721 gactatggga ttgtcaatcc agtccatgcc cttggaaaat tcattcatta catgacaagt
      781 tatcaccctc caacgattcc ccgaacaacc tatagcatca gcgtgattga aggtggaaca
      841 tcggtgaaca cgatcccccg gtcagcccgt tgcgaagttg atctccggtc agtttctgaa
      901 ggtgaacttg atcatctgga acaccatctc cgtgaagcag tcaaacaagc ctgggaagaa
      961 gaagaacgcg ccgccgcact cagccgcacc cggctcaagt taaccgtgac accaatggga
     1021 aaccgtccat ccggagaaac accaaccacc acccgactgg tccgactggc tcaggcagca
     1081 accatgattt gcggatgtcg cccactgctg gactgttctt cgaccgattc aaatttgccg
     1141 atttcgcttt caatccctgc gattacgatt ggggcgggcg ggcgctgcaa cggatgccat
     1201 acgcttgacg agtggtatga acccactgaa cgcgaactca gtttacagcg cgggatcctg
     1261 ttgttgctgg gactggttgg gttagcggaa aagacatcgg gttgaagaca ttgggctgag
     1321 ggcctggggc tgaagaagac gggctgaaga tgttaggttg aaaacagcag tttgaacccg
     1381 ctttcttcag ctccgagctt gcgagtcttc agcccaatat cttcagcccc aggcccgatg
     1441 ttttcagccc gttttcttca gccctggttt ctacaatccc aggccctgag cccgaagttt
     1501 ctcgattctc tcttggcaaa atcacagcta tccagtattg ttggcaggct cttttgagaa
     1561 aagattcaat cattttggtg atcctttcct cagtcgtcag gttgcccctc ttgaatcgct
     1621 tcgcaacagt tcctcaatcg tagaatcttg agttttcaca gagagacgtc cctgggcgat
     1681 tttctctgaa tataattttt ttgtgcttgt gacctctttg cttttttgga gcgtcgttga
     1741 gcagattggc gagtttatga gttcagttgc tgctactgtt tctgtccgtc cccgcttgaa
     1801 ttgggtcaat attacggttt tttctctgct tcatctggct gcatttgcgg cattttttgt
     1861 gcgtccaacc tggacctcac tggcactttt tgtgattttt tatttattga ccgggtttgg
     1921 gatcaccatc gggtttcatc gcctgctggc ccaccggggg tttgaatgct ccaagtttct
     1981 gatccgtctg tgggcatt
//
LOCUS       DAOQDO010001268         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56159_length_1998_cov_1.960885, whole genome shotgun sequence.
ACCESSION   DAOQDO010001268 DAOQDO010000000
VERSION     DAOQDO010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56159_length_1998_cov_1.960885"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..>1998)
                     /locus_tag="PKZ53_17920"
     CDS             complement(454..>1998)
                     /locus_tag="PKZ53_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ42371.1"
                     /translation="MVDGVMLLVDASEGPLPQTRYVLSKALEQNLKSIVVINKIDRQD
                     ARPQEVINEVYDLFIDLDANDDQIDFPIIYAIARDGMAKMNLEDESNDLRPLFEQILK
                     TIPPPLDRREEPLQVLVTNIAYDEYVGRLGTGRIVAGEIKMGDPVMLIKRDRQMERHK
                     ITQLYTFDGLKRVSCERAGAGDIIAVAGIDGINIGETIASIENPIPLPLIQIDEPTIS
                     MIFSVNNSPFSGRDGKYVTSRQLRERLYKEVLGNVSIRVEDTDSPESLKVSGRGELQL
                     SILIEMMRRDGYEMQVSKPEVVTRFEDGVKKEPIELAVIDCPDTFIGVVTEAMGRRKG
                     NMTKMVNHGTGRVRMEFEIPSRGLIGFRSEFLTNTKGTGILNTLFLKWGEWSGVIATR
                     LTGALVADRPGDATAYALYGLQERGELFIRPQTAVYEGMVVGENSREVDLDVNVVREK
                     KLTNMRASSSDEAIRLIPFRELSLEQALEFIRDDELVEITPTAIRIRKRVLAANMRPK
                     RKNEEN"
BASE COUNT          497 a          521 c          466 g          514 t
ORIGIN      
        1 acctccctgg ctcgatcatc cctatggaaa aaccttgata tgaaaaactc tggcaaattg
       61 tatttggatt ggatattgac gtgccacgat accacgttga tccctgcagc gtcaaaacga
      121 ttgctcatca tggtgccagt ttccggctaa ggagcgacgc ccgatgacag agccagcaat
      181 cctggacatc aaaaattggg ggttctggtt gagagtggga gtggaaaaag tgatctggtt
      241 gacggtggtt cgcattctcc gtcaaccaga atttactacg tgatgtagcg cgccagtgac
      301 catatgggct ttcgcttgat tttgtcctca agaaaagctc aattttttct caagccgcga
      361 cctgaccggt gtctgaccga tactgagggc catgaattcg cgaaaacact gagcttcatg
      421 gtgaaaatca agacgatagc gagtcaactc aggttaattt tcttcatttt ttcgctttgg
      481 gcgcatattg gctgccagga cccgtttccg aatgcgaatg gcggtggggg taatttccac
      541 cagttcatca tctcgaataa attccagcgc ctgttcgagc gataattcac ggaaaggaat
      601 taaccggatg gcttcatccg atgaggaagc tcgcatgttg gtcagtttct tttcccgaac
      661 cacattcaca tccagatcca cttcgcgcga gttttccccg acaaccatcc cttcatacac
      721 agcagtttgg ggacggatga acaattcgcc gcgttcctgc aacccataca gtgcataggc
      781 agttgcgtca ccaggacgat ccgcaaccag agccccggtt aatcgagtgg caatcactcc
      841 tgaccattca ccccacttca ggaacagggt attgagaatc cccgtgcctt tggtattggt
      901 taaaaattct gatcggaagc caatcaaccc acgcgaaggg atttcaaatt ccatccggac
      961 gcgtccagtt ccgtgattga ccatcttggt catattgcct ttgcggcgtc ccatcgcttc
     1021 ggtaaccaca ccgataaagg tatctgggca atcaatgacc gccagttcaa tcggttcctt
     1081 tttgacaccg tcttcaaacc gggtgaccac ttcaggtttt gaaacctgca tttcataccc
     1141 gtcacggcgc atcatttcaa tcagaattga aagctggagt tcaccccgac ctgaaacctt
     1201 caggctctca ggcgagtcgg tatcttcaac gcggattgag acattgccca gcacttcctt
     1261 gtacaggcgt tcgcgtaact gccgggaggt gacatatttc ccatctctac ccgaaaaggg
     1321 tgaattattg actgaaaaga tcatcgagat ggttggctcg tcaatctgga tcaggggcaa
     1381 cggaatcgga ttttcaattg acgcaatggt ttcaccaatg ttgatgccat caataccagc
     1441 cacggcaatg atgtcaccag cgccagctcg ttcacaggaa acgcgtttca agccgtcaaa
     1501 ggtgtacagc tgggtgattt tatggcgctc catctgacga tcccgtttga tcaacatcac
     1561 aggatcaccc attttgattt caccagccac aatccgcccc gtgccaagcc tcccaacata
     1621 ttcgtcatag gcgatattgg tcaccaatac ctggaggggt tcttcccggc gatccagagg
     1681 cggaggaatt gtcttgagga tttgctcaaa gagtgggcgt aaatcgttac tctcgtcctc
     1741 cagattcatt ttggccattc cgtcccgggc aattgcataa ataatcggga agtcaatttg
     1801 atcgtcattg gcatcaagat caataaacag atcatacact tcattgatta cttcctgagg
     1861 gcgggcatcc tggcggtcaa tcttgttgat cacgacaatt gattttagat tctgctccag
     1921 tgctttggag agcacatatc gggtttgggg gagcggtcct tctgaagcat caaccagcaa
     1981 catcacccca tccaccat
//
LOCUS       DAOQDO010001269         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56182_length_1997_cov_3.144696, whole genome shotgun sequence.
ACCESSION   DAOQDO010001269 DAOQDO010000000
VERSION     DAOQDO010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56182_length_1997_cov_3.144696"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PKZ53_17925"
     CDS             <1..971
                     /locus_tag="PKZ53_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42372.1"
                     /translation="GFTLAHPMVSSLNYEDIHLIAVFQSGYDSNQCKRYVDIFAPAAN
                     RPVRLDCSFINFDSFQVPPAEDPRERISYFVRILLQRCPHSGVDLATYRFLRDIEHLK
                     TVNNEREVEKYVSRLFEQTEIGNTPESNPQIMLGHEAEPLRDKEDPWMAPPPPPPPPP
                     DPPYAQSGFPSGGPMVSGMSSAPPGGMPMPAQPGFPGAPPPSNMMFNQSQMQAYPNQP
                     GGENYDYDYDQDLTDLITAKKSTQIALGASIGGLVLLMVSLVMPYFWLGIAGSVVGLL
                     QAQKAIRMYERSNQKSNAGSAQGAFVMGIIGFIILVVSVLSFAFKK"
     gene            1043..1936
                     /locus_tag="PKZ53_17930"
     CDS             1043..1936
                     /locus_tag="PKZ53_17930"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNJ42373.1"
                     /translation="MSDLTQQPIKIGIIGGSGLYQMEGLENIREIRVETPFGNPSDAF
                     ISGTLNGVPVVFLARHGRGHYLLPTELPFRANIYALKTLGVEFILSVSAVGSLKEELE
                     PLHMVIPDQFFDRTRARVQESTFFGNGIVGHITFADPVCKYLSDIVEDAGRSLGDVTI
                     HRGGTYLCMEGPAFSTRAESNVYRMWGMDIIGMTNLQEAKLAREAEMSYSTLAMVTDY
                     DCWHQGHSDVTVEMVIEYLNKNVTNAQRIIRGAVERLAQGDLVSPYNSALKHAVMTNP
                     GKITQETRERLAAIIGKYFPA"
BASE COUNT          489 a          518 c          472 g          518 t
ORIGIN      
        1 atggatttac cctggctcac ccgatggtca gcagcttgaa ctatgaagat attcatctga
       61 ttgcagtttt tcagtcaggc tatgactcaa atcaatgcaa acgctatgtg gatatttttg
      121 ctccggcggc caatcgcccg gtacgactcg attgttcttt tatcaatttt gactcatttc
      181 aagtgccgcc ggctgaggat ccacgcgaac gaatttccta ttttgtgaga attttgctcc
      241 agcggtgtcc tcattccggc gtggatctgg cgacctatcg gtttttacga gatattgagc
      301 acctgaaaac tgtcaataat gagcgcgaag tcgaaaaata tgtctcccgg ctgtttgaac
      361 aaactgaaat tggaaatacc ccggaatcca accctcaaat tatgcttgga cacgaagctg
      421 aacccttgag ggacaaagaa gatccctgga tggcaccacc gccaccgcca ccgcctccac
      481 cagatccgcc ctatgctcaa agtggatttc catcaggagg accgatggtg tcagggatgt
      541 caagcgcacc gcctggtggg atgccaatgc cagcgcagcc cggatttcca ggagcacccc
      601 caccctccaa catgatgttt aatcagtccc agatgcaggc gtatccaaat cagccaggag
      661 gagaaaacta tgactatgac tatgaccagg atctcactga tttaatcact gccaaaaaat
      721 caactcagat tgcacttggc gcttcaatcg gcggcctggt tttgttgatg gtaagtctgg
      781 taatgcccta tttctggctt ggaatcgcag gatcagtggt cggcttgctc caggcacaaa
      841 aagccatccg gatgtatgaa aggagcaacc aaaaatcaaa tgccggatcc gctcagggtg
      901 catttgtgat gggcattatc ggttttatca ttctggtcgt gtcagtcctg tcttttgcct
      961 ttaaaaagta agccgcgtga agtcgcgact ttgctatttc acgatttcac tcaatatcaa
     1021 tcaaggagtt atcatcgaac gcatgtcaga tctcactcaa caaccaatca aaatcggcat
     1081 tatcggcggc agtggcctct atcagatgga agggcttgaa aatatccgcg aaatccgcgt
     1141 tgaaacgccc tttggcaacc cgtcagatgc ttttatcagt ggcacactca atggtgttcc
     1201 cgtggttttt ctcgcccgcc acggacgtgg ccactacctc ttaccaactg aactcccctt
     1261 tcgggccaat atctatgccc tcaaaaccct gggcgtcgaa ttcatcctgt cagtttcagc
     1321 cgttggttca ctcaaggaag aactcgaacc cctccatatg gtgattcccg atcagttttt
     1381 tgaccggaca cgcgcccggg ttcaggaatc aacgttcttt ggaaatggaa tcgttgggca
     1441 tatcaccttt gccgacccgg tctgcaaata tctgtctgac attgtggaag acgctggccg
     1501 cagtctgggt gatgtgacga ttcatcgtgg cggcacctat ctgtgcatgg aaggtccggc
     1561 tttctcaacc cgtgccgaat caaacgttta ccggatgtgg ggtatggaca tcattgggat
     1621 gaccaacctc caggaagcca aactggcccg tgaagccgaa atgagttatt ccactctggc
     1681 catggtgacc gattatgatt gctggcatca gggacactct gacgtcaccg tggaaatggt
     1741 catcgaatac ctgaacaaaa acgtcaccaa tgcccagcgg attattcgag gtgcggtcga
     1801 acggctggct caaggcgatc tggtatcacc atataattca gctctcaaac atgcagtcat
     1861 gaccaatcct ggaaaaatta cccaagaaac ccgcgaacga ctggccgcga tcatcggcaa
     1921 atattttcca gcttagcctt tctggttctt ggtttttggt tcttggttct tggttcttgg
     1981 ttcttggttc ttggttc
//
LOCUS       DAOQDO010001270         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56194_length_1997_cov_2.421730, whole genome shotgun sequence.
ACCESSION   DAOQDO010001270 DAOQDO010000000
VERSION     DAOQDO010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56194_length_1997_cov_2.421730"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKZ53_17935"
     CDS             <1..214
                     /locus_tag="PKZ53_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ42374.1"
                     /translation="DKYETLTTREREILQLAAEGSSSTDIAAKLGISARTAETHRTNL
                     MRKLNLHSQADLIRYALRRGIIPMED"
     gene            487..1086
                     /locus_tag="PKZ53_17940"
     CDS             487..1086
                     /locus_tag="PKZ53_17940"
                     /inference="COORDINATES: protein motif:HMM:NF019464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HNJ42375.1"
                     /translation="MSSVNGAVKRPEPVDQLLVQLNDPRTAESLSHLLKNAELLAFLL
                     DAVDGFIRRGDVITDNIAEALKEARPALDGNQMVPADLKLAAVQTMAVLPQLSKTLPQ
                     LVETLPQLVDVGLKFKRVIDSDEFDAFLESGMLAPKTVQLLGQVGDSLVETEKDFKAS
                     PKKVGLFGLWSALSDPDIQLGLGFLVEFGKRLGQKLKNQ"
     gene            1206..1568
                     /gene="hypA"
                     /locus_tag="PKZ53_17945"
     CDS             1206..1568
                     /gene="hypA"
                     /locus_tag="PKZ53_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNJ42376.1"
                     /translation="MHELSIAHSLIEMAGEAAEAAGATSVKTLSLKIGKLSGVVKEAL
                     LFSFEIAAQGTLLEGATLKIEEIPIAVNCPTCARIVVLPEAFIFRCPECDTPTPDIVQ
                     GKELELVSMEILDESANC"
     gene            1549..>1997
                     /locus_tag="PKZ53_17950"
     CDS             1549..>1997
                     /locus_tag="PKZ53_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42377.1"
                     /translation="MNPRIVEIRKGILKKNDQLAAGLRQRFAAAQAQGGTVPEQIAAI
                     ARGVGDIFQVAGSSWNILLEFWAKAKTEPEVAASMVGTLRRYQAMFEALLQRGVSEGS
                     LQAGDTGLLSPWLSMWGANIVLGVIAAALLVLNHREAAFDPLDPAHY"
BASE COUNT          476 a          535 c          535 g          451 t
ORIGIN      
        1 cgacaagtat gaaaccttaa ccaccaggga acgcgaaatc ctccaactcg ccgccgaagg
       61 ttcgagtagc accgatattg ccgccaagtt gggcatcagt gcccgaactg ccgaaaccca
      121 ccgcaccaac ctcatgcgca agctcaatct gcatagtcag gctgatttga ttcgatatgc
      181 gctccgacgt ggaatcatcc cgatggaaga ttgagttgag ggttcagggt tcggggttca
      241 gggtttcaaa agagaacagg acataaagga cataagtgac caaaaggaca taaattcgaa
      301 aaccctgaac cctggtttta aaaaatacgg tggattttgc tgaaaaacta agtagctcta
      361 cgaatagaca gccgacggac ttcgcccaat aatagagcac tcttgaaccg cgtcattttt
      421 cccgaatcct acatttttca agccacttgc ccaacatcgt gattcattca tcaagcggag
      481 gcaaccatga gttctgtcaa tggtgctgta aaacgacctg aaccagttga ccaactgttg
      541 gttcaactca atgatccacg aaccgccgaa tcgctgtcac atctgctcaa aaatgccgaa
      601 ctgcttgcct tcctgcttga cgccgtggat gggtttatcc gtcgcggaga cgtcatcacc
      661 gacaatattg ctgaagcact caaagaagcc cgtccggcgt tagatggcaa ccagatggtt
      721 cctgccgact tgaaactggc agccgttcag accatggcag ttttgccaca actcagcaaa
      781 acactgccgc aactggtgga aacgctgcca caactggttg atgtcggact caagtttaaa
      841 cgcgtgattg attcagatga atttgacgca tttttggaat caggtatgct ggcgccgaag
      901 acagttcaac tcctgggtca agtcggcgac tcgctggttg agaccgagaa agattttaag
      961 gccagtccga aaaaggttgg cttgtttgga ttgtggtcag ccctcagtga tccggacatc
     1021 cagcttggcc tcggctttct ggttgaattt ggaaaacggc tgggacaaaa gctgaagaat
     1081 caataggatt ccgggttccg ggttccgcgc ttccgggttc cgggttccgc gcttccgggt
     1141 tttcgaattt atgtcgtttc ggtcactttc gtcctttcat ttttcctgaa ccctgaaccc
     1201 tatctatgca cgagctttcc attgcccact cactcatcga aatggctggc gaagctgctg
     1261 aagcggcagg ggcaacttcg gtcaaaaccc tgtctttgaa aatcggaaaa ctctcgggcg
     1321 tcgtcaaaga ggcgctcctg tttagttttg aaattgccgc ccaggggacg ctcctggaag
     1381 gcgctaccct gaaaattgaa gaaatcccga tagcggtcaa ctgtccaacc tgcgcccgaa
     1441 tcgtggtgct gcccgaagcc ttcatttttc gctgccctga atgtgacacg ccgacacctg
     1501 acattgttca gggcaaagaa ctcgaactgg tttcaatgga gatccttgat gaatccgcga
     1561 attgttgaaa ttcgaaaagg catcctcaag aaaaacgacc agcttgcggc tgggcttcgt
     1621 cagcggtttg ctgcagcaca ggcacagggt ggcaccgttc ccgagcagat tgcggccata
     1681 gcgcggggcg tcggcgacat cttccaggtg gcgggcagca gctggaacat cctgctggag
     1741 ttctgggcga aggcaaagac cgaaccggag gtcgcggcga gcatggttgg gaccctccgc
     1801 aggtatcagg cgatgtttga ggcgctgctg cagcgtggtg tttcagaagg cagtctgcag
     1861 gcgggcgaca ccggcctcct gagcccgtgg ctgtcgatgt ggggcgccaa catcgtgctg
     1921 ggcgtcatcg cggcggctct gctcgtcctc aaccaccggg aggcggcctt cgacccgctc
     1981 gaccccgcgc actacat
//
LOCUS       DAOQDO010001271         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56360_length_1994_cov_1.983497, whole genome shotgun sequence.
ACCESSION   DAOQDO010001271 DAOQDO010000000
VERSION     DAOQDO010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56360_length_1994_cov_1.983497"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKZ53_17955"
     CDS             complement(<1..744)
                     /locus_tag="PKZ53_17955"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNJ42378.1"
                     /translation="MRKFLSRLVFLVIVFSALSGVDWAQNADLASSAPQPDDRFKADI
                     LVVIAHPDDDSAAAAYLARAVFDQGKRVAVVYTTDGKGGNNQIATERGAALGAVRQIE
                     ARTALAALGINQVWFLQGRDTATQNVLLSLANWGHGQVLEDVVRIIRLTRPEVVMTWF
                     PHAVAGENHGDHQASAVVATEGFDLAGNPTVFPGQLAPPRLRFPTENLQPWQPKKLYF
                     FSDAFDENFMIGKGPEYSVSDVSPARKVPY"
     gene            complement(1107..>1994)
                     /locus_tag="PKZ53_17960"
     CDS             complement(1107..>1994)
                     /locus_tag="PKZ53_17960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ42379.1"
                     /translation="KVDGYTPRPEEDMDTEYNIVETDFFRTMGIQFTAGSDFRLLPPP
                     QTDQPDPNRIEQVIVNETFVKHYLGGGNPLGHQLEVNSQKHQIIGVVKDIKLRSMNES
                     PRPYMYLYVPDNYRSDLILMVKTESDPLTLYPTVRNIVAGLDANIPLFDAKSLAEHTQ
                     LSLFPQRFAMIFLSIFGTLALGLAAMGLYGMMAYSVTQRTREIGIRMALGANKQDVYQ
                     LILGRGLILLLIGMGLGVVGTIAVARLASSLLFGVTATDPLTYLGVSLLLFGISFMAC
                     FFPAYRATRINPVIALRYE"
BASE COUNT          506 a          523 c          526 g          439 t
ORIGIN      
        1 ataggggact ttgcgggcag gagaaacgtc gctgactgaa tattccggtc cttttccaat
       61 catgaaattt tcgtcgaagg catcagagaa aaagtacagt ttcttgggct gccagggttg
      121 aagattttca gtcggaaacc tcagtcgggg tggtgccagt tgtcctggaa acacggttgg
      181 gttcccggcc aggtcaaatc cttcggtggc aaccaccgcc gatgcctggt ggtcaccgtg
      241 attttcgccg gcaaccgcat gcggaaacca ggtcatcacc acttctggac gggttagccg
      301 tataattcgc accacatctt ccagaacctg gccatgcccc caatttgcca gcgacaagag
      361 cacattttgg gtggccgtat cgcggccttg aaggaaccag acctggttga ttccaagtgc
      421 cgcgagggct gtgcgggctt caatttgccg aacggccccc agcgcggcgc cgcgttcggt
      481 ggcgatttga ttgtttcccc cctttccatc agtggtatag accaccgcca cccgcttgcc
      541 ctggtcaaaa acggctctcg ccagatacgc ggcagcggca gaatcatcat caggatgagc
      601 aatgacaacc agaatatctg ctttgaagcg atcatcaggt tggggggcgc tggatgccag
      661 atccgcgttc tgtgcccaat caacaccgga aagggctgaa aaaacgatga ccaaaaacac
      721 aagccgggaa aggaatttcc tcacacgtac ctccacatat tttgtgaatt ttgaactgaa
      781 gcgcgaaaga gccagttgta tcaaccaaca atggcaatcc gccaatatca gccataatgc
      841 cacgggtgtc agaggataaa aaaccaggtt ccctcacctg tgaaactcaa agaacactct
      901 gtgtgagttc aataaatatt tcctcagcgc gcgggcgtcc ccccgcccca gatctattga
      961 tgaaaaacga gttgtgggag ggacgcctgc gcccaagaaa gaatggctgt ttcctgaaga
     1021 attccggcgc tgaagcaccg gtctcaattc agccaccgct acgcggcgaa gaaatgtcaa
     1081 ccgaacctaa acggtttctc aattctttat tcgtagcgga gcgcaatcac cgggttgatg
     1141 cgggttgccc ggtaggccgg gaaaaagcag gccataaacg aaatgccaaa aagcagaagc
     1201 gagacaccca gataggtaag tgggtccgtg gcggtgacac caaataacag gcttgaggca
     1261 agccgcgcca cggcaatcgt tccaaccacg ccaagcccca tcccaatcag gagcagaatc
     1321 aacccacggc ccaaaatcaa ctgatacaca tcctgtttat tggcgcccag ggccatccga
     1381 atgccaattt cgcgtgtccg ttgcgtgacc gaatacgcca tcattccata gaggcccatc
     1441 gccgccagcc caagtgccag agtgccgaaa atactcagga aaatcatggc aaaccgttgc
     1501 ggaaacaggg aaagctgggt gtgttcggcc agtgatttgg cgtcaaaaag cggaatgttt
     1561 gcatcaagtc ccgccacaat gttcctcacg gtcgggtaca gtgtcagtgg gtccgattca
     1621 gttttgacca tcaggatgag gtctgaccga tagttatctg gtacatacaa atacatatag
     1681 ggccggggag attcattcat tgaccggagc ttgatatcct tgaccacccc aatgatttgg
     1741 tgtttctgac tgttgacttc gagttgatga ccaagtggat tgcctccacc gaggtagtgt
     1801 ttgacaaagg tttcgttgac gatgacctgt tcaatgcggt ttggatccgg ctggtccgtt
     1861 tgtggtggtg gcagcaggcg aaaatctgag ccagcggtaa actgaatgcc cattgtccgg
     1921 aaaaagtccg tttcgacgat gttatattcg gtgtccatat cttcttcagg acgtggagta
     1981 tagccgtcaa cttt
//
LOCUS       DAOQDO010001272         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56386_length_1993_cov_2.878741, whole genome shotgun sequence.
ACCESSION   DAOQDO010001272 DAOQDO010000000
VERSION     DAOQDO010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56386_length_1993_cov_2.878741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1519)
                     /locus_tag="PKZ53_17965"
     CDS             complement(53..1519)
                     /locus_tag="PKZ53_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ42380.1"
                     /translation="MKLTRVFPNALTGWSGRLATGGLVLSLCLATPISSSRAATGPFP
                     QEDPKAMAELEAELAHLDGNLAELNGELAAELDQVFAIAGDKQRSFIGVFSDTISDDK
                     ATELGLKKAEGVYIQNVVPGGPAEKAGIKSGDVILEINGTEIVNGEHFRTILGKSEPG
                     SQLKLTIFRDKKKQNITVTTEARGQQQARVYGFGQNGQVLSEEQRKQIEKEMQKAAKA
                     SEKAMKDYEKAFKGNEKALREAEVFAYAFQDAGRLGVSSQSLSEQLSAYFGVKGGVLV
                     TEVESGSAAEKAGVKAGDCITAINGVAINDTKDLRQELHKAEAGNVSLTVVRDKQTLT
                     LNATIEKRQPFKQGALFFPSRGRLGVSVDSLSTQLAQSMGVTNGGVLITEVKSGSAAE
                     KAGLKAGDCITAVNNQPVKDSSDLIEELAKVKEGNVTLSIVRDRQLTTVTATLDKVDE
                     SYLRPLRMTLPTRIQIPPMRIRIPRLSEITIGGSSTLT"
BASE COUNT          451 a          512 c          495 g          535 t
ORIGIN      
        1 cccaatcaaa aaaactgata tgttttgtcc gagttgtgga ctcaatactc gattaggtta
       61 atgtgcttga gccgccgatg gtgatttctg acagccgggg aatccgaatc cgcattggcg
      121 gaatttgaat ccgggtcgga agcgtcatcc ggagcggacg aaggtaggat tcatccacct
      181 tatcaagcgt cgccgtgacc gtcgtgagtt gacgatcacg gacaatgctc aaggtgacgt
      241 tgccttcttt gaccttcgcc agttcttcaa tcagatccga actatcttta acgggctgat
      301 tgttgaccgc ggtaatgcaa tcacccgctt tgagcccggc cttttcagcc gcagaacccg
      361 atttgacctc agtaatcaac acaccaccgt tggtaacacc cattgattga gccagttgcg
      421 tagaaagaga gtcaaccgag acccccagcc gaccgcggct ggggaagaaa agtgcgcctt
      481 gtttgaaggg ctggcgtttt tcaatggtgg cattgagcgt gagcgtttgt ttatcgcgaa
      541 ccacggtcag cgatacattt ccagcttcgg ctttgtgcag ttcctggcgc aggtctttag
      601 tgtcattgat ggcaacccca ttgatggccg taatgcaatc accggcttta acacccgctt
      661 tttcagccgc agagccagat tcgacttcag tgaccagcac tccgcctttg acaccaaaat
      721 aggcgctgag ttgttctgac agagactggc tactcacacc caaccggcca gcatcctgaa
      781 aggcataggc aaagacttca gcttcccgaa gggctttttc attgcctttg aaagcctttt
      841 catagtcttt catcgctttt tcagaggctt tggctgcctt ttgcatttct ttttcgattt
      901 gtttgcgttg ctcctcggac aagacctgtc cattttgacc aaagccatag acccgtgcct
      961 gttgctggcc tcgggcttcg gtggtcaccg tgatattttg ctttttctta tcgcggaaaa
     1021 tggtgagttt gagttgtgag ccaggttcag atttgcccaa aatggtgcgg aagtgctcac
     1081 cattgacaat ttcggtgcca ttgatttcca aaatcacgtc gccagatttg attccagcct
     1141 tctcagccgg accacctggg acaacatttt gaatataaac gccttcagct ttctttaaac
     1201 caagttcggt ggctttgtcg tcactgatgg tatcggaaaa taccccgata aatgaccgtt
     1261 gtttgtcgcc agcaatggca aagacttgat ccaactcagc cgccagttcg ccgttgagtt
     1321 cagccagatt tccatccaga tgggcgagtt cggcttccaa ctcagccata gccttgggat
     1381 cttcttgtgg aaagggcccg gtggccgccc gacttgatga gattggggta gcaaggcaaa
     1441 gtgagagtac cagcccgccg gttgccagac gtcccgacca gccagttaac gcatttggaa
     1501 acacacgtgt caatttcatg cagcaattgc tccttcgttt cttcttgaga ttattttgct
     1561 tttggcctgt gttgaggagt gatctgtaaa cagttgtgaa tgcagtctgg cgatgcgcac
     1621 gctgtacggg gtgaaaagtt ccgcaaaaat cttcagacag aagacatcgg gcggaagacg
     1681 tcgggcagaa gaaaacaggt ggaagactcg caagctcggg gcggaagaaa atgggctgaa
     1741 gaaggcggat tcaataccct gagcccgaag cattcagccc aatgtcttga gccggcgtct
     1801 tctgccccca ggcccaagtt cttcattctt cattcttcat tctcaattct gagcggggtg
     1861 cttgactttc gcgctttgtc acttttactc tgttttagca cgcttaccgt tcacaactct
     1921 ttattgctcc agatttcaag ttacccggca ccggcggttt tctatgatgg aaacccaata
     1981 ccggctgcaa aca
//
LOCUS       DAOQDO010001273         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56420_length_1992_cov_5.255034, whole genome shotgun sequence.
ACCESSION   DAOQDO010001273 DAOQDO010000000
VERSION     DAOQDO010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56420_length_1992_cov_5.255034"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PKZ53_17970"
     CDS             complement(<1..134)
                     /locus_tag="PKZ53_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ42381.1"
                     /translation="MDARVRRPTHPGEILREDILPAAGLSQTELAARIGVSRKIISEI
                     L"
     gene            complement(515..1900)
                     /locus_tag="PKZ53_17975"
     CDS             complement(515..1900)
                     /locus_tag="PKZ53_17975"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42382.1"
                     /translation="MSVPVVSRFVFAFWLVFWGWLAGSGPAFGMQSGPIPPIQSGYGA
                     NGPYSVKVETLPSPQFSGQNVTVYFPTETTTPLPVVFFSHGFGAINPRTYEPFIRHIA
                     SRGYIVVYAPYPFTSPTIQERYQVLLAGFVEAASTYRSRMDLSRVGFVGHSFGAGATP
                     AIAYQGITQQGWGSAGVFLFMLAPWYSYDITPEQLSNFPPQTKLIVQVYDDDAVCDHR
                     MAKDIFTHISIPDSEKDYVVLFSDRSEALDYQLKADHLTPTATNPGRSGDRSNALDFY
                     GVWRLFDALAEYTFQGTSDAKIVALGNGNVDQSNMGTWPAPENRPVKPLLATDSPVVT
                     HPESDFAYGFSNVLNPRRGFGDVNTASPVVSNVTLSNTKIIRRRDSTVTITWTSTDDQ
                     QVISHDLSFASTGTDFSVGIVSGLSGTAQAFTWRLPTDIQPTKTGVVKVVATDAAGNS
                     GQALSGRIKIK"
BASE COUNT          514 a          464 c          539 g          475 t
ORIGIN      
        1 agaatctcgc tgatgatctt gcgagagacg ccaatgcggg cagctaattc ggtttgggaa
       61 agcccggcgg cgggcaaaat atcttcacgt aaaatttcgc cggggtgggt tggtcgtcga
      121 acacgtgcat ccatcataag ttcaatcctt taatgataat cctcaagatc aacatgaaag
      181 gcgtctccat cattaaattg aaaggtaatg cgccagttcc cgctcacctt tatgccgaaa
      241 gctgatgatt attcgaaggt tgtaacttaa aaaacagtct cagtaaagtt attcgtttgg
      301 tgtagaaaac accgtaggcc gataatgaga aagtcgaaga tatagcattt cagataaata
      361 tttcctggga gcgccggcat cttgccggca tcaatcggtt gcggttcaag cagttgccgg
      421 caggatgccg gcgttcccag gggggaatct ttttctgaaa aactatagcc tcaacggatt
      481 gaggatcttt ggtttcgtcg ttcttccaat tggctcactt aattttaatt cggcccgaca
      541 gtgcctgacc agaattccca gccgcgtctg tggcaacgac tttgaccacg ccggttttgg
      601 ttggttgaat gtcggttggg agtctccagg taaatgcctg ggctgtccct gaaagtcccg
      661 aaacaattcc aactgaaaaa tcggtgccag tgctggcaaa gctgaggtcg tgggaaatca
      721 cctgctgatc gtcagttgaa gtccaggtga tggtgacggt tgagtctcta cgtctgataa
      781 tctttgtatt tgataaggta acatttgaaa cgactggtga tgcggtattg acgtctccga
      841 atccgcgccg gggattgagc acattgctga atccataagc aaaatcactt tcgggatgcg
      901 tgacaactgg cgaatcggtt gccagcagcg gcttgaccgg gcgattttca ggcgccggcc
      961 aggtacccat attcgattga tcaacatttc cgtttcccag tgccacaatt ttggcatcgg
     1021 acgttccctg aaacgtgtat tccgccaggg catcaaacaa tcgccagacg ccatagaaat
     1081 caagcgcgtt ggatcggtct cccgaacgac cggggttggt tgctgttggg gttaaatgat
     1141 cggctttgag ttgataatcc agggcttcgg acctgtcgct aaacaacaca acataatctt
     1201 tttcactgtc aggaatcgaa atatgggtga aaatgtcttt ggccatacgg tggtcacaga
     1261 cagcatcgtc gtcataaacc tggacaatca gtttggtttg aggcgggaaa ttgctcagtt
     1321 gttcaggcgt gatgtcatag gaataccagg gcgccagcat aaacaaaaac acgccggcgg
     1381 atccccaacc ctgctgggtg attccctggt aggcaatggc tggcgtcgca cctgcgccaa
     1441 acgaatggcc gacaaaccca acccgactta aatccatacg cgaacgatag gtactggctg
     1501 cttcaacaaa tccagccagc agcacctgat atcgttcctg aatggtcggg ctggtaaacg
     1561 gatagggggc atagacgaca atgtagccac ggctggcgat gtgtctgatg aacggttcgt
     1621 aggtccgagg attgattgct ccaaacccgt gggagaaaaa cacgactggt aacggcgtgg
     1681 ttgtttcagt cgggaagtaa accgtgacgt tttggccgga aaattggggg ctcgggaggg
     1741 tttcgacctt gacggagtag gggccgttgg caccatatcc agattgaatt ggaggaattg
     1801 gcccggattg cattccgaaa gccggccctg agccagccag ccatccccaa aaaacaagcc
     1861 agaacgcgaa cacaaaacga cttacaacag gaactgacat atccaaatgg tcctcaccgc
     1921 gattgaagtt tgagggggat gagttcggac tgtctggtta gagccgcaag gtagtcaaaa
     1981 agttgcgcaa at
//
LOCUS       DAOQDO010001274         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56489_length_1991_cov_3.014979, whole genome shotgun sequence.
ACCESSION   DAOQDO010001274 DAOQDO010000000
VERSION     DAOQDO010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56489_length_1991_cov_3.014979"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1991
                     /locus_tag="PKZ53_17980"
     CDS             <1..>1991
                     /locus_tag="PKZ53_17980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ42383.1"
                     /translation="LGRAFVPEEEQAGAPLVVILSHAFWKRQFAGDPALLGKTINCNA
                     QPYTVVGIMPPGFTWGRYYGNEESADLWVPFQLLPERLGDANRGSEYLDMYARLKPGV
                     TLAQAHAEVDGLVRGIHQRLEWTHGTETGLEIKVRQIQEEITSEVRPLLTVLLAAVGM
                     VLLIACANIANLILVKIGQRQSEIAVRMALGAGKWRIIQQLLTEALMLSIISGLAGLG
                     IAWVLTETIQSADLPNVPRIQDISFNWVVALFGIGITILTGCLCGIAPAIRAGSKPLT
                     EYLRSQGRIISQDRVWNLFRNGLAVTQIALAVTLMAGSGLLIVSLYHLLSVNPGFQTR
                     NLLTLEVSLPDKLYDSNAKKATFFQNSTQAIRQLPGIQNVDVVTDMPLSGSSSTNTIT
                     IEQYQVNPGDTGPTGEYFSVTPGYFQTMGIPIKQGREFTDQDRADSKLVVVIDEVMAR
                     RYWPNENPIGKRFHRQENDDNPIWREIVGVVGQIKNRSLNDIERGQYYMPVAQVPAGR
                     ANFMVRTANDPLSHIEAIKAAIHSVDPQLPIFKIRTMDTIVAKSLTNQRLSTWVLGAF
                     AAVALLLSSIGIYGVLSISVVQRFQEFGIRMALGAKPADVFQLVLGQGIRLIAFGVVI
                     GLSLAYFATRLLESVIFGVKPSDPVVLIGVTLVLGLVAV"
BASE COUNT          443 a          485 c          561 g          502 t
ORIGIN      
        1 tgctcggacg tgcctttgtg ccggaagaag aacaggctgg cgctccactg gtggtgatct
       61 tgagccacgc cttctggaag cggcagtttg ccggtgatcc ggcactgctt ggaaagacga
      121 tcaactgcaa tgcacagccc tataccgtgg ttggaatcat gccgccaggg tttacctggg
      181 gaaggtatta tggaaacgaa gaatcggctg acctgtgggt tccgtttcag ctcctgcctg
      241 aacgattagg tgatgccaat cgcggaagcg aatatctgga tatgtatgcc cggctcaaac
      301 ccggagtgac gctggctcaa gcgcatgcag aagtggatgg gctcgttcgt ggtattcacc
      361 agcggctcga atggacccat ggcactgaaa ccgggcttga aatcaaagtc cgccagatcc
      421 aggaagaaat caccagcgaa gttcgcccgt tgttaactgt gctgttggct gcggtgggta
      481 tggtgttgct gattgcctgt gccaacattg ccaatttgat cctggtgaaa atcggccagc
      541 ggcagtcgga aattgctgtc cggatggcgc tgggtgccgg taaatggcgg attattcagc
      601 agttgttgac tgaggcattg atgctttcga tcatcagtgg attggccgga ttgggcattg
      661 cgtgggtttt gacagaaacc attcagtcgg cggatttacc caatgtgcca cgtatccagg
      721 acatttcatt caactgggtg gtggctttgt ttggcattgg catcacaatc ctcaccggct
      781 gtttgtgcgg cattgccccg gccattcggg ctggttccaa gcctcttacc gaatacctcc
      841 gctcacaggg gcgaatcatc agccaggatc gagtctggaa tttgtttcgc aatgggctgg
      901 ccgtgaccca aattgctctg gccgtgacat tgatggccgg gtccgggctg ttgatcgtca
      961 gtttgtatca cttgttgtcg gtcaatccgg ggtttcaaac caggaatttg ttgactcttg
     1021 aggtgtcgtt acctgacaag ctgtatgact ccaacgccaa aaaagcgacc ttttttcaaa
     1081 actcaactca ggcaatccgg cagcttcccg gaatccaaaa cgtggatgtc gtgaccgaca
     1141 tgccactcag cggatcgtca tcaaccaaca ccatcacaat tgaacagtat caggttaatc
     1201 caggtgacac cggtccaacg ggcgaatatt tttcagtcac acccggttat ttccaaacga
     1261 tgggcattcc catcaaacaa ggacgagagt ttactgatca ggacagggcc gattcaaagc
     1321 tggtcgtggt gattgatgaa gtcatggcac gtcggtactg gccaaatgaa aatccaatcg
     1381 gcaaacgctt tcatcgtcag gaaaatgatg acaatcctat ctggcgggaa atcgtggggg
     1441 tggtcggaca gatcaaaaac cgcagcctca atgacatcga acgcgggcaa tattacatgc
     1501 cggtggccca ggtgccagcg ggtcgagcaa attttatggt ccgaaccgcc aatgatccat
     1561 tgtcgcacat tgaagccatt aaggcggcca ttcattcggt tgacccacaa ttgcccatct
     1621 tcaaaatcag aaccatggac actattgttg cgaaatcact gaccaatcaa cggttatcaa
     1681 cctgggtact cggtgctttt gcggcggtgg ctttgctgct cagttcgatt gggatttacg
     1741 gggtgctttc aatttcggtg gttcagcgct ttcaggaatt tggaatccgg atggcgttgg
     1801 gggccaaacc agccgatgtc ttccaactgg tgttgggtca agggatcagg ttgattgcct
     1861 tcggagtggt cattggcctt tctctggcat attttgcgac ccgcctgctg gaatcggtca
     1921 tttttggggt caaaccgagt gatccagttg ttctgatcgg cgtcacgctg gttctggggc
     1981 tggttgccgt g
//
LOCUS       DAOQDO010001275         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56535_length_1990_cov_3.259948, whole genome shotgun sequence.
ACCESSION   DAOQDO010001275 DAOQDO010000000
VERSION     DAOQDO010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56535_length_1990_cov_3.259948"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1563
                     /locus_tag="PKZ53_17985"
     CDS             154..1563
                     /locus_tag="PKZ53_17985"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNJ42384.1"
                     /translation="MTTHELINAMTMPELILQVIKLHEEMAELRAENERLRAEIEELK
                     RNGNRSAAPFSKGKGKNKGLKAGRKAGEGKFEYRGIPAPETITEGVVEVKVEGVGCPA
                     CRGELERVRVEMAYRTEIPVEVKPEVTAYEIEVCRCRKCGREVRGEPPEVAKDQRGAT
                     AHRVGERVMAVAHTLHYEYGIPLRKVPGIVKDLTGVGVTQGALTQDAHRRAAGEIGAA
                     YEELRGSVKQSRQVHTDDTGWRVEGEPAYLMVFETEAATVYQIRDRHRNEEVREVIPE
                     EYAGTMITDRGRSYDAVELSGVKQQKCLAHILRSIAEVLATKVGPSRTFGVRLKHLLQ
                     AALEVWRMHRQVTLADFEAQAAELKAKITDHLRDRKLPDADNQRLLNELGRHHDAGNL
                     VRFLDDPTLEPTNNRAERALRPAVIARKVSHCSKNEAGARTFEAFKSVTQTIKRTGHS
                     VVDGLYELFRRHKLQAASP"
     gene            complement(1588..>1990)
                     /locus_tag="PKZ53_17990"
     CDS             complement(1588..>1990)
                     /locus_tag="PKZ53_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42385.1"
                     /translation="LGPRNIMLWINSPTAVIQIPRLNDAPLDFDWLFSCLQSVNQCPA
                     RCFIFDFTNCDFLKPNAVAFLGGVARWIERQGKEFVFDWRTLQQNIRVNLTQNGFLKT
                     FGGSQFGAWDGNSVPYREDYQKNKNDLMDYL"
BASE COUNT          550 a          439 c          599 g          402 t
ORIGIN      
        1 aaactcctgg actgcccggg cgcgcaataa aaaatagagc acggcatttg gaacctggtc
       61 atcgggcttt tgtttataaa tctgcagtaa ttgattagcg gcattatcca agaaagcttg
      121 aaagaaccag aagttatttt taaagtcaag ccaatgacaa cacatgaact tatcaacgcc
      181 atgacgatgc cggagttgat tctgcaggtg atcaaactgc atgaagagat ggcggaattg
      241 cgggcggaaa acgagcgatt gcgagccgag atcgaggaat taaaacgaaa tgggaatcgg
      301 tcggcggcgc cgttttcaaa agggaaaggt aaaaacaaag gactcaaagc gggccggaag
      361 gcaggagaag gaaagttcga gtatcgggga atcccggcac cggaaacgat cacggaaggg
      421 gtggtggaag tcaaagtgga aggggtcgga tgtccggcgt gccgagggga gttggagcgg
      481 gtgagagtgg agatggcgta tcggacggaa attccggtgg aagtcaaacc agaggtaacg
      541 gcgtatgaga tcgaggtgtg ccggtgtcgg aagtgtgggc gggaagtgag aggggaaccc
      601 ccggaagtag cgaaggacca gcggggagcg accgcgcatc gggtgggaga gcgagtgatg
      661 gcggtggcgc atacgctgca ttatgagtat ggaattccgc tgaggaaagt gccggggatc
      721 gtgaaggatc tgaccggggt gggggtgacg caaggggctc tgacacagga tgcgcaccga
      781 cgggcagcgg gagaaatcgg agcggcctat gaggaattga gaggaagcgt gaagcagtcg
      841 cgccaggtgc ataccgacga tacggggtgg cgggttgaag gagagccggc atatttgatg
      901 gtgtttgaaa ctgaggcagc gacggtgtac caaatccgag accgccatcg caatgaggaa
      961 gtgcgggagg tgattccaga ggagtacgcg ggaacgatga tcaccgaccg gggtcgcagt
     1021 tatgatgcgg tggagttgtc aggggtcaaa caacaaaaat gcctggcgca catcctgcgg
     1081 tcaatcgccg aagtgctggc gaccaaagtc ggacccagcc ggacgtttgg agtgcggctc
     1141 aaacatctgc tccaggccgc gctggaggtg tggcggatgc accgccaggt gaccctggcg
     1201 gattttgagg cgcaagccgc cgaactcaaa gccaaaatta cggatcactt gcgagaccga
     1261 aaactcccgg atgcggacaa ccagcggttg ctcaatgaac ttggtcggca tcacgacgct
     1321 ggaaatttgg tgcgtttttt agacgatcca accctggaac cgaccaacaa tcgggcggaa
     1381 cgtgcgctgc gtccagcggt gattgcccgc aaagtctccc attgttccaa aaatgaagcc
     1441 ggcgcccgaa cctttgaagc tttcaaaagt gtgactcaga ccatcaagcg caccggtcat
     1501 tcggtggttg atggattgta tgaattattt cgccgccaca aactccaggc cgcctcacct
     1561 tgaattctcc ataaagccgc taatcaatta caaataatcc atcagatcgt ttttattttt
     1621 ttggtaatcc tcacgataag gaactgaatt cccgtcccat gccccaaact ggcttccgcc
     1681 aaaggttttt aagaaaccat tttgagtaag atttacacgg atattttgtt gtaaagtcct
     1741 ccagtcgaaa acaaattcct ttccttgtcg ctcaatccat cgagccactc caccaagaaa
     1801 agcgacagca ttcggcttta aaaaatcaca gttggtaaaa tcaaaaatga agcatcttgc
     1861 aggacattga ttaacagatt gtaaacagga aaatagccag tcgaaatcca aaggcgcgtc
     1921 gtttagtctt ggaatttgaa ttactgctgt tggtgaatta atccacaaca taatgttacg
     1981 tggtcccagt
//
LOCUS       DAOQDO010001276         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56538_length_1990_cov_3.099742, whole genome shotgun sequence.
ACCESSION   DAOQDO010001276 DAOQDO010000000
VERSION     DAOQDO010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56538_length_1990_cov_3.099742"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKZ53_17995"
     CDS             complement(<1..489)
                     /locus_tag="PKZ53_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ42386.1"
                     /translation="MQLTVAEAAHLLQVSEETVYKWIRVENLPALLFNGQFYFNQVRL
                     VEWAHANHIAVAVESSSDLPSLESAFQPDNLLANIAGATISEVLQQIVGQLPIPHQAD
                     REFVSQMLLTRKQFGITAFGNGIGIPRARSPIVLPNLKPSVVLSYTRTPFQFGAADRQ
                     PLR"
     gene            complement(859..>1990)
                     /locus_tag="PKZ53_18000"
     CDS             complement(859..>1990)
                     /locus_tag="PKZ53_18000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNJ42387.1"
                     /translation="KALRNIPANELMDAVVNLIIQISGFGFCGVLEYDLNEDTAAFKL
                     GGGWEDRRFRELTWQGEENAFWSNVVHEQQPVYGEMVPGNVPLTIPAQLQILGEFRSL
                     STLIPGSSHPFQILVVFSPAQTCFGPEDSYFLQAVANILATAFERKRAEENRSRLLER
                     MMSSQEQERRWIARELHDETGQSLTSLLVGLRLIMDASSLKAAREQADRLRKITIQTL
                     DNIGRLARGLHPSILDDLGLVIAVNRYAAEYQTSYGIETQTVSQGMDQPELPASIKIT
                     LYRIFQEALTNVVKHAQATRVTISLNRQSDTITLQIKDNGIGFDLESVLRSTSNSLHL
                     GLLSMRERANLLGGSLQIVTAPNCGTTVTAVIPLTKTILARQ"
BASE COUNT          475 a          504 c          509 g          502 t
ORIGIN      
        1 ccgcaacggc tgcctgtcag ccgctccgaa ttgaaacggg gtacgggtat aactcaaaac
       61 aacagaaggt ttgaggtttg gaaggacaat cgggctccgg gcgcgaggga ttccaattcc
      121 gtttccaaag gcagtaattc caaattgttt gcgggttaag agcatttgag agacaaactc
      181 tcgatctgcc tgatgcggga ttggcaactg cccaactatc tgctgcaaga cttcggaaat
      241 ggtcgctccc gcaatattgg ccaggagatt gtccggctgg aaagctgatt caagcgaggg
      301 caaatcagaa gagctttcaa cggccactgc aatatggttg gcgtgcgccc attcaaccag
      361 tcgtacctgg ttgaaataga attgaccgtt gaacaacagc gcgggcaggt tttccacccg
      421 gatccatttg taaacggtct cttctgatac ttgaagcaag tgggctgctt cggccaccgt
      481 taactgcata cctgtatttc aaaccattga aagctctctg tttgcttctg gcgggtgaag
      541 ccagtcacaa aggggcgttt gacgatacag tgcgccgaaa atgacactcc gtctagcggg
      601 gaaaatcctg aaaattggtg attttgtaag ggttatcctg acaagggcat cgggttccgg
      661 gtttctcaag ggatgaggga tgaaggatga gggatgaaaa aatcacccgg tcaccttgtc
      721 accttgtcac cttgtcagtt ggtcatcttg tccgggttcc ggagttttcg aagttctatc
      781 ctttttgtcc cttttgtctt tttcgtccct ttcgtccttt tctttccctg aaccctgaac
      841 cctatcccca aagtgcaatc attggcgggc caaaatggtt ttggtgagag gaattacggc
      901 tgtcacagtg gtgccgcaat tgggggcagt cacaatctga agactccctc caagcagatt
      961 ggcccgttcg cgcatgctca acaggcccaa atgaagagaa ttcgaggtgc ttcgaagtac
     1021 cgattccagg tcaaagccga ttccgttatc tttgatctgc aaggtgattg tgtcggactg
     1081 acggtttaaa ctgatggtaa ctctggttgc ctgggcatgt ttgacaacat tggtgagcgc
     1141 ttcctgaaaa atccgataca acgtgatttt gatcgaagcc gggagttccg gctggtccat
     1201 gccctggctc acggtttggg tttcaatgcc ataactggtc tgatattcgg ctgcgtagcg
     1261 attgacggca atcaccaacc caaggtcatc gagaatgctt ggatggagcc cgcgtgccag
     1321 acggccaatg ttatcaagcg tttgaatcgt tatcttccgc aaccggtcag cctgttcgcg
     1381 tgcggccttc aaactggaag catccataat cagtcgcaac ccaaccagca gcgaagtcag
     1441 tgattggccg gtttcgtcgt gaagttcgcg ggcaatccag cgtcgttcct gttcctgaga
     1501 tgacatcatg cgttccagta atcgagaccg attttcttcc gctcgtttgc gctcaaacgc
     1561 ggtcgccaga atattggcga cggcttgcaa aaaatagctg tcttcagggc caaaacaggt
     1621 ctgggcggga gaaaaaacaa ccaggatctg aaacggatga gaacttcctg gaatcaaggt
     1681 ggacaaactc cggaattccc ccaatatctg cagttgcgcc gggatggtca gcggaacatt
     1741 ccccggaacc atttcaccat aaacgggttg ttgttcgtgt accacgttgc tccagaacgc
     1801 attttcttca ccctgccagg tcagctctcg aaatcgtctg tcctcccaac ccccaccaag
     1861 cttgaacgca gccgtgtctt cattcaggtc atattccaac acgccgcaaa atccaaaccc
     1921 tgaaatttgg atgatgagat tcacgacggc atccatcaac tcgtttgctg gaatattgcg
     1981 taatgccttc
//
LOCUS       DAOQDO010001277         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56581_length_1989_cov_3.748190, whole genome shotgun sequence.
ACCESSION   DAOQDO010001277 DAOQDO010000000
VERSION     DAOQDO010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56581_length_1989_cov_3.748190"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKZ53_18005"
     CDS             complement(<1..1029)
                     /locus_tag="PKZ53_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNJ42388.1"
                     /translation="MAQHSGADIVIQALVRNGVDTVFGYPGGAIMPLYDAFYDAPLQH
                     ILTRHEQAAVHAAEGYSRVTGKVGVCIATSGPGATNLITGLADAWMDSVPIVAITGQV
                     ATHLIGTDAFQETNVVGISTAITKQTLLPRTVEELSTAIAEAFYIARSGRPGPVIIDI
                     PKDVLIKKTDLPLATEAGTRIPGYRHRPELNTELAIEAMRLLQESEKPLILVGGGCKM
                     SGPETVELFRQFCDRTGIPVTSTVHGLGSTAYEYPYDLGMLGMHGLRRANKSVGKCDL
                     LIALGMRFDDRVTGAPDRFAKQAKIIHVDIDQAEINKLIPVDLGIHADLGEVLALWLE
                     ELDQEPYQT"
     gene            complement(1181..1357)
                     /locus_tag="PKZ53_18010"
     CDS             complement(1181..1357)
                     /locus_tag="PKZ53_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42389.1"
                     /translation="MNRSQNTFQTTSRKTRSGFLSLTLGVVNTVVSTLATASVVILVI
                     VGIISVIMTAAVAS"
     gene            complement(1428..>1989)
                     /locus_tag="PKZ53_18015"
     CDS             complement(1428..>1989)
                     /locus_tag="PKZ53_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNJ42390.1"
                     /translation="RQKVTSVDKANVLETSQLWREIVNEVAREYPDIALEHMYVDNCA
                     MQLILHPERFDVVLTENLFGDILSDEAAVLAGSLGMLPSASLGDGSGLYEPVHGSAPD
                     IAGQGKANPIGAILSVAMMLRHSFHLEAEAKLIETAVSVALDNGFRTADLTDAYRAIS
                     TSDFGKRVCQYVEAGIKDPVLWFEFA"
BASE COUNT          463 a          549 c          531 g          446 t
ORIGIN      
        1 ggtctgatag ggttcctgat ccagttcttc cagccagagc gcgagcacct cgcctaaatc
       61 agcatgaatg cccaaatcaa ccgggatgag cttgttgatt tcagcctgat caatatccac
      121 gtggatgatc ttcgcctgtt tggcaaagcg gtcaggggcg cctgtcaccc ggtcatcaaa
      181 gcgcatgcca agggcaatca acagatcgca cttgccgacg gatttgttgg cgcggcgcag
      241 accgtgcatc ccgagcattc cgaggtcata tgggtattcg tacgctgtcg aacccaaccc
      301 atgcacggtt gacgtcacag ggattccagt ccggtcacaa aactgccgga agagttccac
      361 ggtttccgga ccggacattt tgcatccgcc acccaccaaa atgagtggtt tttctgattc
      421 ctgcagcaga cgcatggcct ctatcgccag ttcggtattg agttccggac gatgccggta
      481 gcctggaatc cgggttccgg cttcggtggc cagcggcagg tcggtttttt tgatcagaac
      541 atctttggga atatcaataa tcaccgggcc aggccgacca gaacgggcaa tgtaaaatgc
      601 ctcggcaatg gcggttgaga gttcttcgac cgtccgtggc agcagtgttt gtttggtgat
      661 ggccgtcgaa atcccgacca cattggtttc ttgaaacgca tcggtgccga tcaggtgtgt
      721 cgccacctga cccgtaatcg ccacaattgg taccgaatcc atccaggcat cggccagccc
      781 ggtgatgagg ttggtcgcac ccggcccgga ggtcgcaatg cacaccccga ctttgcccgt
      841 gactcgggaa tagccttcgg cggcgtgaac ggcggcctgt tcgtgacgag tgagaatgtg
      901 ttggagtggc gcgtcgtaga acgcgtcata gaggggcata atcgcaccgc ccggataccc
      961 aaagaccgtg tcaaccccgt tcctgacgag tgcttgaata acgatgtcag caccgctatg
     1021 ctgtgccata tacggttttc tcctagaggc aaaattaggt gatcagaagc gtttcagcag
     1081 gactgccctc ctgctgaaac cgccgtagaa aatgaaaaag ccacctgctc gcgtccggcg
     1141 gcaggtggct ttgagttttt aactcgttgt tcctaagagc ttaggaagcc accgccgccg
     1201 tcataatgac gctaataatc cctacgatta ccaggattac taccgaagca gtggccagag
     1261 tagacacaac tgtattcacg acaccgagcg tgaggctcaa gaatccagac cgggttttcc
     1321 gtgatgttgt ctgaaaagtg ttttggctac gattcataag gtgattcctt cgcggaagcg
     1381 aaaggcgcgt gattggcgct cagactactc cgcttttttt gattcagtca agcaaattcg
     1441 aaccagagca ccggatcttt tatcccggcc tccacatact gacacacccg tttgccaaaa
     1501 tcagacgtcg aaatggcccg gtacgcatcg gttaaatccg cggttcgaaa gccattatcg
     1561 agtgcgacag aaacagccgt ctcgatcagt ttggcttcgg cttcgaggtg aaatgaatgg
     1621 cggagcatca tggccaccga taaaattgcc ccaatcggat tggccttccc ctgcccggca
     1681 atatcggggg ccgagccatg aacgggttca tacagccctg aaccatcgcc cagactggct
     1741 gaagggagca tcccgagcga accagccagc acggcggctt catcgctcaa aatatcgcca
     1801 aagagatttt cagtcagcac cacatcaaac cgctcaggat gtaaaatgag ctgcatggcg
     1861 cagttgtcca catacatatg ctcaagcgcg atgtctgggt actcacgggc cacttcgttg
     1921 acaatttcac gccagagttg ggacgtttcc aacacattgg ctttatcaac tgaagtcact
     1981 ttctggcgg
//
LOCUS       DAOQDO010001278         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56716_length_1987_cov_3.163043, whole genome shotgun sequence.
ACCESSION   DAOQDO010001278 DAOQDO010000000
VERSION     DAOQDO010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56716_length_1987_cov_3.163043"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKZ53_18020"
     CDS             <1..327
                     /locus_tag="PKZ53_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42391.1"
                     /translation="PSPLEKAQAVLRQQVQHQQERARYQLHRLFKPHSFVRRHLFGLL
                     ASACLLVAGANLFWPITGTTDPTALAQAEIIIHLRSIEWLMLGLLSLAGGLYFKPTDD
                     FRRITR"
     gene            671..1540
                     /locus_tag="PKZ53_18025"
     CDS             671..1540
                     /locus_tag="PKZ53_18025"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ42392.1"
                     /translation="MSKHRLLQITLIGFLSLTFGQSALAQEAPAGDTPPRRVVITNRD
                     FGVDDAPARPAPAPETTTTTAGSEAPATEVKKEEPEWEVAPKTIMNVVKDKDGRWVVT
                     STQVPGKAKVLVVGTDRTISTGKPELDKLITEAGQKHGVDPLLIMEVMRQESGFRQYA
                     VSPAGAKGLMQFIDGTARRYGITDPFDPQQSIDGGARYLRDLLEMFDGNVELALAGYN
                     AGENRVKRSGNQVPNIRETQAYVKNITSKYGSKQHYATKNASKKAQPAELPPPPPRVV
                     QTNDGTLLFTNRE"
     gene            1628..>1987
                     /locus_tag="PKZ53_18030"
     CDS             1628..>1987
                     /locus_tag="PKZ53_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42393.1"
                     /translation="MKGSNTDPGAARYQLEVALLHLAQAAEGHRLHLKLTLPDHIQHF
                     EQRPETICCEVSYQKLILSFWGDTYAQSWQVVSFTSTLNRIELQVSRQSGRLMGSLLI
                     QVDLADHLLVPPERQGQD"
BASE COUNT          512 a          531 c          489 g          455 t
ORIGIN      
        1 ccatcaccac ttgaaaaagc acaggcggta ctccgccaac aggttcaaca tcagcaggag
       61 cgggcgcggt atcaactcca ccgactgttc aaaccccatt cttttgtacg tcgccatttg
      121 tttgggttac tggccagtgc ctgtttactg gttgctggtg ccaatctttt ctggccgatt
      181 acagggacga ctgacccaac tgccctggcc caggctgaaa ttatcatcca tttgcgcagt
      241 atcgaatggc tgatgctggg gctcctgagt ttagcaggtg gcctgtattt taaaccgact
      301 gatgacttca gacggatcac cagatagcac ctgacagcgt gaccgggtga taactgacaa
      361 aataacacct gtcatgttgt cacccgggac cagaggttgt cccagatatt gacactttca
      421 acgtaaatcc ctacactttt gaccatctgt atccagaaat cctaaaatat catcccaaac
      481 agtctttcct gcacttatcc ctttcgtgac ttcaagtccg cgcccaggtt gggcttcgcc
      541 tcagggtttt tctggagcag gtaacttcac catcagtgca ccaacaaaaa tcaagattta
      601 attcattttt ggcactgatt ttgctcaact taaatgcaat ttggattttg ttgaaaggac
      661 ccgaacccga atgagtaagc accgcttgct tcaaattacg ctgattggat ttttgtcttt
      721 gacctttgga caaagcgctc tggctcagga agcaccggca ggtgatactc cgccacgacg
      781 ggttgtcatt accaaccgtg acttcggagt tgacgatgct ccggccaggc ctgccccagc
      841 cccagagaca accaccacca cggctggatc tgaagcacca gccacggaag tgaagaaaga
      901 agaaccagaa tgggaagttg cccccaagac catcatgaat gtcgtcaagg ataaggatgg
      961 ccgctgggtg gtaaccagca cccaggtacc agggaaagcc aaagtcctgg tggttggcac
     1021 tgatcgaacc atttcaaccg ggaaacctga actggacaag ttgattacag aagccggtca
     1081 aaaacacggt gttgatccac tgctgattat ggaagtgatg cgtcaggaat caggattccg
     1141 ccagtatgcg gtttctcctg ctggtgcgaa aggattgatg cagtttattg atggtacggc
     1201 tcgtcgctat ggcatcaccg acccatttga cccacaacag agtattgatg gaggcgcccg
     1261 atacttgcgc gatttgctgg aaatgtttga tggaaacgtc gaactggcac tggccgggta
     1321 caatgcgggt gaaaaccggg tcaaacgcag tgggaatcaa gtccccaata tccgtgaaac
     1381 ccaggcctat gtgaaaaaca tcacctccaa gtatggcagc aaacagcact acgcaacgaa
     1441 aaatgcttcg aaaaaagctc aaccggctga actgccacca ccgccaccgc gtgtggttca
     1501 aaccaatgat ggaacgctcc tgtttaccaa ccgcgagtag gttctggaac acaatttgcc
     1561 ctttcgtcag gcacagccca ttccctgggc tgtgcctttt actttttgga aagggtgatg
     1621 aaggcacatg aaaggttcga atacagatcc tggagcggcc agatatcaac tggaggtggc
     1681 tctgctccat ctggcacagg cagccgaagg ccaccggctt caccttaaac tcacgctccc
     1741 ggaccacatc caacactttg aacagcggcc agaaacaata tgctgcgaag taagctatca
     1801 aaaactcatt ctttcatttt ggggtgacac ctacgcccaa agctggcagg tggttagttt
     1861 tacgtccact ctcaaccgga ttgaactcca ggtctcgcga cagagcggga gactgatggg
     1921 ttcgctcctg atccaggttg atctggccga tcacctcctc gtgccgccgg agcggcaggg
     1981 acaggac
//
LOCUS       DAOQDO010001279         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56737_length_1987_cov_2.245342, whole genome shotgun sequence.
ACCESSION   DAOQDO010001279 DAOQDO010000000
VERSION     DAOQDO010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56737_length_1987_cov_2.245342"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="PKZ53_18035"
     CDS             <1..1864
                     /locus_tag="PKZ53_18035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ42394.1"
                     /translation="KQVLYISPAYELVWGRTCQSLYDSPTDWLEAIHPEDRDRVEQQF
                     LVQAVGKYDVEYRIIRPDGSLRWIHDRGFPIADRDGKIYRIAGVAEDITERRQAETVS
                     QQQQEQLEGIVNSAMDGIITINEDQQIILINAAAERMFGCRAGEALGKGIDQFIPERF
                     RAAHAGHVRQFGQTGIAARAMGLYGRISGLRSDGEEFPIEASISQIQVEGRKLFTVTC
                     RDVTERVRTAAEKQKLEAQLHQSQKMEAFGQLAGGVAHDFNNLLTVISGYSELLLTML
                     PGYDSRRAMVIEIRDAGERAATLTRQLLAFSRQQVLEPKVLDLNTIVTDTEKMLRRLI
                     GEDVEFSTVLRPNISPVKVDPGQIIQVILNLAVNARDAMPQGGKLTIETSDIELSESY
                     LKLHPDAQPGQFVMLAISDNGSGMAPEIKNRIFEPFFTTKGVGKGTGLGLAVVHGIVK
                     QSGGNIDLYSEVGVGTTFKIFLPAVQERPESRLDTGLPGSLRGKETILLVEDEDIVRN
                     ITSFSLEEFGYTILTAPDGKTALKILAHHEGKIDLLITDVVMPEMSGRELAETLLSQN
                     SKLKVLFMSGYTDDAIVRHGVLQAHVAFLQKPFTPTSLAKKVREVLDHDQVTNK"
BASE COUNT          496 a          510 c          549 g          432 t
ORIGIN      
        1 caaacaggtc ctctatataa gccccgcata cgaactggtc tggggacgca cctgccagtc
       61 actctatgat tcacccacgg attggctgga agcgattcac ccagaagatc gtgatcgggt
      121 ggagcagcaa tttttggttc aagcagttgg caaatacgat gttgaatacc ggatcatccg
      181 tccggatggc tcactgcgct ggatacacga ccggggtttt ccgattgctg accgcgatgg
      241 aaagatttac cgcattgccg gggtggcgga agacatcacc gaacgccgac aagccgaaac
      301 ggtctcacaa caacaacaag aacagcttga gggaattgtc aattcggcga tggacgggat
      361 cattacgatc aatgaggacc agcagattat cctcatcaac gccgctgccg agcgaatgtt
      421 tggttgccgt gccggggagg cgctgggaaa aggcatagac cagttcattc cagagcgatt
      481 ccgtgcggct catgccgggc atgtgcgaca gtttggccag accgggattg ccgcccgggc
      541 catggggctg tatggccgga ttagcggcct gcgttctgac ggggaagaat tcccaataga
      601 agcttcgatt tcacaaatcc aggttgaagg ccgcaagctg tttaccgtga cctgccgcga
      661 tgtcaccgag cgggtccgaa cagccgccga aaaacaaaag ctcgaagccc agcttcacca
      721 gtcccaaaaa atggaggcat ttggacaact ggcgggcggt gtggctcacg actttaataa
      781 tttgctgacg gttatttccg gctatagcga actcttgctg acgatgctgc ctggatatga
      841 ttcccgaaga gccatggtta ttgaaatccg tgatgccggg gaacgagcag caaccttgac
      901 tcgccagctt ctggccttta gccgccagca ggtacttgaa cccaaagtgc tcgatctcaa
      961 cacgattgtg acggatactg agaaaatgtt gcgccgattg attggtgaag atgtggaatt
     1021 ttcaaccgtg ctacgaccaa acatcagtcc tgtaaaagtt gatcctggtc agatcattca
     1081 agtcatcctg aatctggcgg tcaacgcgcg cgatgccatg ccacagggtg ggaagctcac
     1141 tatcgaaacc agtgacatcg aattgagcga atcctacttg aagctacatc cggacgccca
     1201 accaggacag tttgtgatgc tggcgatcag tgataacggt tcgggcatgg cgccggaaat
     1261 caaaaaccgt atctttgagc cgtttttcac caccaaaggt gttgggaaag ggaccgggtt
     1321 ggggctggcc gtggtgcatg gcatcgtcaa acagagcgga ggcaacattg acctctacag
     1381 cgaagtcggc gttggtacca ccttcaagat ttttctgccc gccgtccagg aacgacctga
     1441 gtctcggctg gacaccgggt taccaggttc acttcgtggg aaggaaacga ttctcctggt
     1501 cgaagatgaa gatattgttc gcaacattac ttcgttctcg ttagaagaat ttggctacac
     1561 catactcacc gccccagatg ggaagacggc gctcaaaatc ctggcacatc acgaggggaa
     1621 aattgatctg ttgataacgg atgtggtcat gcctgagatg agcggtcgtg aactggctga
     1681 aacgttgcta tcacaaaatt caaagctgaa agtactcttt atgagcggct acactgatga
     1741 tgccattgtt cggcatgggg ttctgcaagc ccacgtggct ttcctgcaaa aaccattcac
     1801 gccaacttcg ctggccaaaa aagtgcgcga ggtcctggac cacgatcagg taactaataa
     1861 atgatggaag aacaacctgt gtgctgattc agtcaataga ctcggccctc aagggccgag
     1921 cgtgagtcgt gcacccacga cgccatcctc gcggatggcc caccgtggtg gcgttgcctc
     1981 acgcttc
//
LOCUS       DAOQDO010001280         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56763_length_1986_cov_3.225790, whole genome shotgun sequence.
ACCESSION   DAOQDO010001280 DAOQDO010000000
VERSION     DAOQDO010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56763_length_1986_cov_3.225790"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1986
                     /locus_tag="PKZ53_18040"
     CDS             <1..>1986
                     /locus_tag="PKZ53_18040"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42395.1"
                     /translation="QRAEPLYQRALAIREKALGPDHPEVADVLNDLANLYWTTGDYQR
                     AEPLYQRALAIREKVWGADHPVTASNLTNLANLFSSLGNYQKAESLYRRALAIREKTL
                     GPDHSQVAVTLNNLANMFYAAGNLPQAIQCQIRANDASESDLKRNLVAGSEQQKVLYL
                     KQTSSYTDVILALHLQSAPQSREARQAALTALLRRKGRALDAMTTAIETLRQQQTPEI
                     QQLLENYANLAGQISALTLRGPDKQTPEEHLAYVKELETQKDRLEAEISTKSTEFKTQ
                     VTPITLENIQKQLPSDGVLIEFTTYKSYTPKTFQFGTLRMAVYTLDHAGTIRWADLGE
                     AAPIEQAVMAFRKAVSTPRTDQIKDVVPAAQALDRLVMKPVRALIGDTRHLLISPDGV
                     LNLIPFAALMDEQGKFLVETYMLTYLTSGRDVLRLAVKNESQQPPLVITDPDYLDGTG
                     PQMLGHQLGRLARLAGTRVEGEQLKTIFPNTVLKVRAEATEQDLKHVKRPLLVHIATH
                     GYFLEDAPQPSETTSADQLDRNIEKARFNPEKERQANPLLRSMLFFAGANQGGTQDND
                     GVMTALEAAQLNLWGTKLVVLSACDTGLGDVKNGDGVYGLRRALVLAGSEAQMISLWP
                     VSDQATRELMVDYYTRLKAGEGRSQALRNVQLKFLKK"
BASE COUNT          531 a          543 c          529 g          383 t
ORIGIN      
        1 atcaacgcgc cgaaccactt taccagcggg cgctggccat ccgggaaaaa gcgctggggc
       61 ctgatcatcc ggaagttgct gacgtgctta atgatctggc gaatttgtat tggaccacag
      121 gagactatca acgggccgaa ccactctacc agcgggccct ggcgatccgg gagaaagtgt
      181 ggggagctga ccatccggta acggccagta acctcaccaa tctggcaaat ctcttcagca
      241 gcctggggaa ttaccagaaa gccgaatcac tttaccggcg ggcgctggcc atccgtgaaa
      301 aaacgctggg acctgaccac tcacaggttg cggtaacgct caacaattta gcgaatatgt
      361 tttacgccgc aggtaatttg ccacaggcca ttcaatgtca gattcgagcc aatgatgcca
      421 gcgaatcgga tttgaagcgc aacctggtag caggttctga acagcagaaa gtcctctatc
      481 tcaaacaaac gagcagttac accgatgtaa tcctggcgct gcatcttcaa tcggcgcccc
      541 aatcgcgtga ggccagacag gcagccttga cggcgctgtt gcgtcgcaaa gggcgggcac
      601 tggatgccat gacgacggct atcgaaaccc tgcggcaaca gcaaacacct gagattcaac
      661 agcttctgga gaattatgct aacctggctg gtcaaatttc ggcactgacg ttgcgtggtc
      721 ctgacaaaca aacacccgaa gagcatctgg cttatgtcaa agaattggaa acccaaaaag
      781 acaggctgga agctgaaatc agcaccaaaa gcactgaatt caaaacccag gtaacaccaa
      841 ttaccctgga gaacattcag aaacaacttc cgtctgatgg agtgctgatt gaatttacca
      901 cgtacaaatc ctataccccc aaaacatttc aatttggaac attgcggatg gcggtctaca
      961 cactggacca cgcggggaca atcaggtggg cagatctcgg agaggcggcc ccgattgaac
     1021 aggccgtgat ggcctttcgc aaagcagtga gcacccccag gacagaccag atcaaagacg
     1081 ttgtcccagc cgctcaggcg ctggacaggc tggtcatgaa acccgtgcgg gcgctgatcg
     1141 gagatacccg ccacctgttg atctcccctg acggtgtatt gaatttgatt ccctttgctg
     1201 ccctgatgga tgaacaggga aagttcctgg tcgaaaccta catgctgacc tatttgacca
     1261 gcgggcgtga cgtgctgcga ctggcggtca aaaacgaaag ccagcaaccg ccgctggtga
     1321 tcactgaccc ggattatctg gatggaaccg gaccacaaat gctgggtcac cagcttggtc
     1381 ggctggcacg acttgccgga acccgagttg aaggcgagca gctcaaaacc atctttccga
     1441 acacagtgtt gaaggtacga gctgaagcaa ccgaacaaga cctgaaacac gtcaagcgac
     1501 cactcctggt ccacattgcc acccacggct attttctgga agatgcgcct caaccatcgg
     1561 aaacgacttc tgccgatcaa ctggaccgaa acatcgaaaa agccaggttc aatcccgaaa
     1621 aagaacgaca agccaatccc ctgctccggt caatgttgtt tttcgctggt gccaatcaag
     1681 gcggcacaca agacaacgac ggggtgatga cggcgctgga agcggcccaa ctcaatctct
     1741 ggggcacgaa actggtcgtg ctttcagcct gtgataccgg gctcggcgat gtaaaaaatg
     1801 gtgatggggt gtatggtctc agacgggcac tggtgctggc tggaagcgaa gcccagatga
     1861 taagcctctg gccggtttcc gatcaggcaa cacgggaatt gatggtggac tattacaccc
     1921 gactcaaagc tggtgaaggc cgttcccagg cgttgcgcaa tgtgcagctc aagttcctca
     1981 aaaaac
//
LOCUS       DAOQDO010001281         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56767_length_1986_cov_3.003107, whole genome shotgun sequence.
ACCESSION   DAOQDO010001281 DAOQDO010000000
VERSION     DAOQDO010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56767_length_1986_cov_3.003107"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="PKZ53_18045"
     CDS             complement(<1..1354)
                     /locus_tag="PKZ53_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ42396.1"
                     /translation="MVALRLPHFVISLILFAASSVFWISVRPTSVSFPPLQSASFSIV
                     ERSPVVNEGNQLRLTVVDATGQPVSGVVWESGSPEIVTVEPQTGVLQGVLQGVATVTA
                     RRNAERTSVFVVVTRVSSTHGATVPGETKVDTRGRVYLTDPINSVVLRKESLVAPAQL
                     FAGSASAHGHRDGNRLGAWFAGPTAATIDNGALGGVYVADTLNHCIRKVGYDDQVSTV
                     VGNGTPGILTADITPLSSAVLRGPRGVAIDTGGNLFVADTENNAIFYVDFNRQEIRLL
                     AGMPGESGLADGTGRTARFAHPRGLALSPDGRLLAVADEGNRVVRLVSKSGTVKTLGR
                     QSAAREWKTTSEDEFLFQAPQSVSFDGQGNLCLIDKAEAYVVINPETSSPERIPLAQP
                     GVSFGQAVSVTVRGYQTFVLDAQAASSDQAVKVVTIGAPRIKTISRTSDRIEGGAEVV
                     V"
     gene            1811..>1986
                     /locus_tag="PKZ53_18050"
     CDS             1811..>1986
                     /locus_tag="PKZ53_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ42397.1"
                     /translation="MPNYKLTINQQKVTVSVEPEMPLLWVLRDYLGLTGTKFGCGLGV
                     CGACTILQGNQSVRA"
BASE COUNT          499 a          562 c          496 g          429 t
ORIGIN      
        1 caaccaccac ttcggcgccg ccttcgatcc ggtcagaggt ccggctaatg gttttaatgc
       61 gcggcgcgcc aattgtcacc actttgactg cctggtcaga actggccgcc tgggcatcaa
      121 gcacaaacgt ctgatagccc cggacagtca cactgaccgc ctgcccaaat gacacgccag
      181 gttgagccag cgggatccgt tcgggagagc tggtttccgg attgatcaca acgtaggctt
      241 cggccttgtc aatcaggcac agatttccct gcccgtcaaa gctgaccgat tgtggcgctt
      301 gaaacaaaaa ttcatcctca gaagttgttt tccattcgcg tgcggcagat tggcggccaa
      361 gggttttgac cgtcccggat tttgaaacca gtcggaccac ccggtttcct tcatcagcca
      421 ccgccagcaa gcgtccatct ggactgagcg ccagccctcg cggatgggca aaccgggcgg
      481 tgcggccagt tccatctgcc agacctgact caccgggcat tccagccagc aaccggatct
      541 cctgccggtt aaaatcaaca taaaagatgg cgttattttc ggtatcggct acaaacagat
      601 tgccaccagt atcaatcgcc acgccacgtg gaccgcgcaa aaccgcgctt gaaagcggtg
      661 tgatgtcagc cgtcaagatt cccggggtac catttcccac cacggttgag acctgatcgt
      721 catacccaac cttgcgaatg caatgattga gcgtgtcggc cacatacacc ccacccagcg
      781 cgccattgtc aatggtggca gcggttggcc cggcaaacca ggcacccagg cgatttccat
      841 cacggtgacc gtgtgctgac gctgaaccgg caaaaagctg agccggtgcc acaagactct
      901 ccttgcgcaa aacaacacta ttgatcgggt cagtcagata gactcgccca cgggtatcaa
      961 ctttggtttc acccggcacc gttgcgccgt gagtggaact gactcgcgtc acgacgacaa
     1021 acactgaagt acgctcagca ttgcgacgag ccgttaccgt ggcaaccccc tgcaagacac
     1081 cctgcaggac tccggtttgg ggctcaacgg tcacgatttc cgggctcccg gattcccaga
     1141 caacacccga aaccggttga ccagtcgcat ccaccaccgt caatcgaagt tgatttcctt
     1201 cgttgaccac gggtgagcgt tcaacaattg aaaatgaagc cgattgaagc ggaggaaacg
     1261 aaacagatgt cggacggaca gaaatccaaa atactgagct agcggcaaac agaatgagag
     1321 aaataacaaa gtgcggcaat ctcaaggcga ccataactac caccttctct ggcaagtaat
     1381 gcactacaaa tcagaattat tggggattga aagatcgttc ggttgcttac cagtttagaa
     1441 tgccaaagtt aaacgcaccg tgaataaccg ctttgaatag gcaaatttgg cgggatttcc
     1501 cggtacaaat ccgggatttt tcagactggg gtttgaattg taactggatt gatcttgctt
     1561 ctgtagtttg ggttcacaat tcaagtgact gtaaacaatg tgtaaattcc cagccatgca
     1621 tctggtgcaa tctgaatttc tcggctagag tcggtttcta ccagaccgac cacaagaagt
     1681 tcctctactt cgaattccct tcgctcaatt catcctcaag atttctaaaa agtgaaagct
     1741 gcgtcaagcc gcccgctttg aaagctgcgt caagccgcag cactccaaag cgagccgcag
     1801 cactccagat atgccaaatt acaaattgac catcaaccag caaaaagtaa cggtttcggt
     1861 cgaaccggaa atgccactcc tgtgggtatt gcgtgattat ttgggactga ccggaaccaa
     1921 gtttggctgc ggccttggcg tgtgcggcgc ctgtaccatt ttgcagggga accagtcagt
     1981 gcgtgc
//
LOCUS       DAOQDO010001282         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56822_length_1985_cov_3.388083, whole genome shotgun sequence.
ACCESSION   DAOQDO010001282 DAOQDO010000000
VERSION     DAOQDO010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56822_length_1985_cov_3.388083"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PKZ53_18055"
     CDS             complement(<1..1187)
                     /locus_tag="PKZ53_18055"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNJ42398.1"
                     /translation="MGEQNIKFELEGEQLRVFTKALLNDLRALEYLIENNLIESEITR
                     IGAEQELFLVDQSFQPAPVAIELLDLLKDDHYTTELAKFNLEINLDPLVFENRCFSVL
                     EKQTLDLLNRIRQQGREIGVHPILVGILPTIRKADLELENLTPKPRYLALNRILNSIR
                     GQEYEFYIRGVDELCVRHDSMMVESCNASFQVHLQVKAEEFPQRYNIAQALAAPVMAV
                     SGNSPLLFGRRLWSETRIAVFRQSVDTRASSQFLRERSTRVTFGSQWVKNSITELYKE
                     DISRFRPLIGGELDEDAMDVVLSGDIPKLKALKLHNGTVYRWNRACYGISEGKPHLRI
                     ENRVFPSGPTVVDEVANAAFWLGLMEGMPKHFPDVSQLMNFEDAKQNFYTAARLGMLS
                     QFT"
     gene            complement(1219..>1985)
                     /locus_tag="PKZ53_18060"
     CDS             complement(1219..>1985)
                     /locus_tag="PKZ53_18060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ42399.1"
                     /translation="DNFEVEVLRAEEHLKQAVLDRRGTRTVNSFKWEKIVSRGVPTSV
                     LLQEATRHQVDLIVMRSGRHTNTTILESTSEYLCRTAPCPILITHFKDRDWISPEHDE
                     VILKRVLVAYDFSADSELALSYGFSLAQEFQAELHVLHVLPHSPKSEVPEIAGLPTID
                     EIAFQKVIAALRSAVPSDASPWCEIRQVVRIGYPYREILNYADEAQIDLICMGSSGTG
                     FGPKALFGSNSDRVLRQSPCSVLIARPSGSQVGASH"
BASE COUNT          523 a          521 c          461 g          480 t
ORIGIN      
        1 caggtaaatt gggacagcat tccaagccgg gcagccgtat aaaaattctg cttggcgtct
       61 tcaaaattca tcaactgcga gacatccgga aaatgctttg gcatcccttc cattaaacca
      121 agccagaagg cagcattggc cacttcatca accacggtcg gtccagaggg gaatacccga
      181 ttttcaatcc gtaaatgtgg tttcccctca ctgatcccgt aacaggcacg gttccagcga
      241 tagacagttc cgttgtgcaa cttcaaggct ttcagttttg gaatgtcgcc actgagcacc
      301 acatccatgg catcttcgtc taattcgcct ccaatgagtg ggcgaaaacg ggaaatatct
      361 tctttataca actcagtgat tgaatttttc acccactgtg agccaaaggt gacgcgggtg
      421 cttcgttcgc gcagaaactg gcttgaagcc cgtgtgtcca ctgactgtcg aaaaacagca
      481 atgcgggttt cgctccacaa ccgacggcca aataacagag gtgagttacc agacaccgcc
      541 atcacaggtg ccgccagtgc ctgggcaata ttgtagcgct ggggaaattc ctccgctttc
      601 acttgcaaat ggacctgaaa actggcgttg catgattcaa ccatcatcga atcgtgtctc
      661 acacagagtt cgtccacccc acgaatatag aactcatact cctgtccacg gatggaattt
      721 aatatccggt tgagtgccag ataccggggt ttgggcgtta aattctcaag ctcaaggtct
      781 gctttccgga tcgtcggcaa aatcccaacc aggatcgggt gaactccaat ttcgcgccct
      841 tgctggcgaa ttcgattgag caagtccagc gtttgttttt ctaaaaccga aaaacaccga
      901 ttttcaaaga ccagaggatc aagattgatt tcaaggttaa acttggctag ctcagttgtg
      961 taatggtcat ctttgagcag atctaacaac tcgatagcaa caggcgctgg ttgaaaactt
     1021 tgatcaacca aaaacaattc ctgctcagca ccaattcgag tgatctccga ttcaatcaag
     1081 ttgttctcta tcaggtattc gagcgctcgc aaatcattga gaagcgcttt tgtaaagaca
     1141 cgtaactgct ctccttcaag ttcaaatttg atattttgtt cgcccatggc gtcggcatcc
     1201 ttcgaggaaa tcaaacagtt aatgtgaggc cccaacctgg gaaccggatg gtcgtgcgat
     1261 taaaacagaa cagggagact ggcgcagcac ccggtctgaa ttggatccaa acagggcttt
     1321 tggtccaaaa ccagtcccgc tgctccccat acaaatcaaa tcaatctgcg cttcatcggc
     1381 atagtttaag atttcgcggt acgggtagcc aatccgcacg acctgccgaa tttcacacca
     1441 cgggctggca tcagaaggca ccgcactccg cagggcagca atcacctttt ggaaagcaat
     1501 ttcatcaatc gtaggtaacc cggcaatctc aggcacttct gacttgggac tatgcggcag
     1561 cacatgcagc acgtgcaatt cggcctgaaa ttcctgggcc aacgaaaacc cataggataa
     1621 tgccagttct gaatcggctg agaaatcgta ggcgaccagc acccgtttga ggatgacttc
     1681 atcgtgctca gggctaatcc aatcccggtc cttgaaatga gtaatgagaa tcggacaggg
     1741 cgcggtgcgg cataaatatt cagaggttga ctccagaatc gtggtattgg tatgccgacc
     1801 tgaccgcatc acaatcaaat ccacctgatg tcgggttgcc tcctgcagga gcacactggt
     1861 tggcactcct cgggacacga ttttttccca tttaaaggaa ttgaccgttc gtgtcccacg
     1921 gcgatccaat actgcttgct tgaggtgctc ttccgcacgc agaacttcaa cctcaaaatt
     1981 gtcgg
//
LOCUS       DAOQDO010001283         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56830_length_1985_cov_2.565285, whole genome shotgun sequence.
ACCESSION   DAOQDO010001283 DAOQDO010000000
VERSION     DAOQDO010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56830_length_1985_cov_2.565285"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..145
                     /locus_tag="PKZ53_18065"
     CDS             14..145
                     /locus_tag="PKZ53_18065"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42400.1"
                     /translation="MHCMPLRRNVEIADDVMESAQNLCYDEAENRLHIQKEILRALI"
     gene            238..525
                     /locus_tag="PKZ53_18070"
     CDS             238..525
                     /locus_tag="PKZ53_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNJ42401.1"
                     /translation="MSRNQSASQRENRLSIRIKPQQKELIVRAAALKNTTVSDFVVEQ
                     AVSAATEILADQTHFVLPPEQWEAFCQALDAPPKQNPQLAALFSRRSVFDE"
     gene            518..1036
                     /locus_tag="PKZ53_18075"
     CDS             518..1036
                     /locus_tag="PKZ53_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ42402.1"
                     /translation="MSDNVLKSPVLLSRDHNRELFDCGVPALNEFLKKYALQNQKKDA
                     ARTYVAVRGNRIAGYYTLAYGSIAPSEAAKKLAAGLGQYPIPVMLLARLAVDVTERGR
                     GLGADLLRDALLRTIQAAEIAGLRALIVHAKDDAARAFYEKFGFEPSPSNLYHLFLPI
                     SEIRKTIGREEP"
     gene            1105..1620
                     /locus_tag="PKZ53_18080"
     CDS             1105..1620
                     /locus_tag="PKZ53_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HNJ42403.1"
                     /translation="MEELSVLKEHIRAGRYGDALTVIEELEEMSRQDKLNAIHSFLIR
                     LLTHLIKCYAEGRLTGSWAASIASSVDEIRTRNRRDNGTHYIKSGEWEAQVEQAFRRA
                     LREAAVEAFGGISPAILSERIDHTTVMAKAVIFLELSYEKNQDQFDAELDDHLAALPG
                     GDAWIGRRGEI"
     gene            1617..1916
                     /locus_tag="PKZ53_18085"
     CDS             1617..1916
                     /locus_tag="PKZ53_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42404.1"
                     /translation="MSSLVQPQLAIPTEKEALSFVNRWLHRKIGMALHSTKATFDPVS
                     FYWHLPVELAYPDRGTIGTIGDLYLHAITGSFAGIPDPEELKNRAESLSTLQGIE"
BASE COUNT          515 a          475 c          511 g          484 t
ORIGIN      
        1 aacggcaaag tttatgcact gtatgccgct ccgccgaaac gtcgaaattg ccgatgacgt
       61 gatggaatca gcccagaacc tttgttatga cgaagctgaa aaccggctgc atatccagaa
      121 agagattttg cgggcgttga tttgatctcg ctcactgctt tttctgcgaa agattgaccc
      181 gacatcaaac cgggcgcaca atgggtgtac acagttcagt tcaaggagtt ttcgggtatg
      241 agtagaaatc aatctgccag ccagcgggaa aatcggttga gcattcggat taaaccccag
      301 caaaaagaac tgattgtccg ggcagccgca ctcaaaaata caacggtcag tgattttgtg
      361 gtcgaacagg ccgtcagtgc tgcgacagaa atcctggcag atcaaaccca cttcgtctta
      421 cccccggaac aatgggaagc cttttgtcag gcactggatg ctcctccaaa acagaaccct
      481 caattagcag cgttgttttc ccggcggagt gtttttgatg agtgacaacg tgctgaaatc
      541 accggtactg ctctcacgtg accacaatcg agagcttttt gattgtggtg ttccggcgct
      601 caatgaattt ctcaaaaagt acgctcttca aaaccagaaa aaggatgccg cacggaccta
      661 cgtcgccgtc aggggcaatc gcatagctgg atattacacg ctggcctacg gttctatcgc
      721 gccgtcagaa gcggcgaaaa aacttgccgc cgggctgggg caatatccaa tcccggttat
      781 gcttctggcg cggctggcgg ttgatgtgac tgaacgagga cgcgggctag gtgcggacct
      841 gcttcgggat gccttactca gaacgattca ggcggctgaa attgccggat tgcgggctct
      901 gattgttcat gcgaaagatg atgcggccag ggctttttat gaaaaatttg ggtttgaacc
      961 ttcacccagt aatctgtacc atctgtttct cccaatctca gagatcagga aaacgattgg
     1021 acgtgaagaa ccgtgacgag gtagcgtatt tccaactctt cacccgtcgt actgactgct
     1081 gactacgccc tggagaatca atgtatggaa gaacttagcg tcttaaaaga gcatatccgt
     1141 gctggtcggt acggtgatgc cctgacagtc attgaggaac tcgaagaaat gagcagacaa
     1201 gataagttga atgccattca ctcgtttctc atcagattat taacccatct catcaaatgt
     1261 tatgccgaag gaaggctgac cggctcgtgg gcagcttcga ttgccagttc agttgatgaa
     1321 atccgaaccc gcaaccggcg cgataacggc acgcattaca ttaagtctgg agaatgggaa
     1381 gctcaggtgg aacaggcttt tcgacgggca ctccgtgaag cagccgttga ggcatttggc
     1441 ggaatttctc cggctatact ctcagaacgc attgatcaca caacagttat ggcgaaggca
     1501 gtcatttttc tggaactgag ttatgaaaag aatcaggatc agtttgacgc agaacttgac
     1561 gaccatctgg cggccttgcc tggcggtgat gcatggatcg gcagaagagg tgagatatga
     1621 gttctttggt ccaacctcag cttgcaattc caaccgaaaa agaagccctt tcttttgtga
     1681 accggtggct tcatcgcaaa attggaatgg cactccattc gacaaaagca acttttgacc
     1741 cagtttcttt ctattggcat ttgcctgttg aactggctta tccggaccgg ggaacaatcg
     1801 gcaccattgg cgatttgtat cttcatgcga ttacgggttc atttgcaggc attcctgacc
     1861 ctgaagaact gaagaatcgt gctgaatcac tttccacact ccagggtatt gaatgaggaa
     1921 gcatgcccat ctcagtcatt aaatttggtg gtgaagtcct gaaccagccg gaaacgctgg
     1981 caacg
//
LOCUS       DAOQDO010001284         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56865_length_1984_cov_3.347330, whole genome shotgun sequence.
ACCESSION   DAOQDO010001284 DAOQDO010000000
VERSION     DAOQDO010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56865_length_1984_cov_3.347330"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..>1984
                     /locus_tag="PKZ53_18090"
     CDS             366..>1984
                     /locus_tag="PKZ53_18090"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42405.1"
                     /translation="MRLVTHALVSLILTMVIGMTIEAGQTGRTIEYALTPGVPNSHLI
                     EVQITYVPPTVVTSVDFAIPAWRPGRYKIQNYARNVQEFSAVNERNQQLKTEKIDKNT
                     WRVNAQSVKQVTVRYKVYANVFDAGSTLLTEDELYWNGTNLLMYVVGHKDVPARLRVN
                     VPSSWRIASGLQKTGEPNVFLAPSYDVLADAPGIASPTLDIVSFDHDGARYFLAFQGE
                     VDYDKRALAENLKRTVVEALKIFGKAPFTEYWFLFHVLPDARYHGVEHLNSTSITVPS
                     STFTTGWGLNRFYSISTHEFFHVWNVKRIYPQVLASIDYSQEVYTRNLWFAEGITTYY
                     DDLLCKRAGLVTVAGYLDGLAGNITTQQNSIGRTLTSAAQASFDEWLTPDDAVNVETD
                     IYTKGSLLGLLLDMQVRHRTDNQKTLDDVMRYLNATYAQKQAGYPEDGIQKALEAVTG
                     SSFAEFFESYVAGVKELPYKEFLDLAGVEVVEGRTIDTPESLFGVTFGGDEKQVTLTQ
                     VRPDEAGFRAGLAKGDILAAIEFEQVTQASLA"
BASE COUNT          462 a          496 c          507 g          519 t
ORIGIN      
        1 agcccttagc cccacagctt cagccccaag ccttcagccc gaagttgttc ttttccttcc
       61 tgcacgttcc ttccattttg ttcgttgcct cctgcctttt ggttgatctc tgctgttcag
      121 gccgcccgtg gttttcggaa agcgacctgc ttgccgaaaa cacaattcac acaccatgcg
      181 ttgccactgg ttttcagggg actggttcga tgtccttcca tcctggcaaa tgcagaaaaa
      241 taagaactat gattttcggt aactgcggtt ggagagacct tcaaaaaccc aggaccctgg
      301 gtttagaagg gagcgaaagc caaatttttc aattctgtcg tgtttggcga caacgaggtt
      361 ctgatttgag actggtaacc catgcgcttg tttccttgat tttgaccatg gtgatcggaa
      421 tgacgattga agctggccag actggccgaa ccattgaata tgccctcacg cctggagttc
      481 caaattcgca cctgatcgaa gttcaaatta cctatgtccc gccaactgtg gtgacgtcag
      541 ttgattttgc cattcctgcc tggcggcctg ggcgctataa aattcaaaac tatgcgcgca
      601 atgtccagga gttctcagcc gtgaatgagc ggaaccagca actcaaaacc gagaaaatcg
      661 acaaaaacac ctggcgagtc aatgctcagt cagttaagca agtaaccgtt cgctataaag
      721 tatatgccaa tgtgtttgat gccgggtcaa cgctcctgac tgaagatgag ttgtactgga
      781 atggcaccaa tctgttgatg tatgtggttg ggcacaaaga tgtgcctgcc cggttgcgcg
      841 tcaatgttcc atcttcctgg cggattgcca gcggcttgca aaaaacaggt gaacctaatg
      901 tatttctggc gcccagctat gatgtgctgg ccgatgcacc agggattgcc agtccaacac
      961 ttgatatcgt gtcatttgat cacgacggcg cccggtattt cctggccttc cagggggagg
     1021 ttgactatga caaacgagcg ctggcagaga atttgaaacg aaccgtggtc gaagccctca
     1081 agatttttgg caaagcaccc tttaccgaat actggtttct gtttcatgtt ttgcctgatg
     1141 cccggtatca cggtgtcgag cacctcaatt cgacttcgat taccgtgccc tcatcaacct
     1201 ttaccaccgg gtgggggctc aatcgttttt attcaatcag tacccacgag tttttccacg
     1261 tctggaatgt caagcggatc tatccgcagg tcctggcctc aattgattac tcacaggagg
     1321 tttacactcg caatctgtgg tttgccgaag ggatcaccac ctattatgac gatctgctgt
     1381 gcaagcgtgc cgggttggtt accgtcgccg ggtacctcga tgggctggca ggcaatatca
     1441 caactcagca gaattcaatt ggccgcacac tcacttcggc ggctcaggcc agttttgatg
     1501 aatggttgac tccggatgat gcggtgaatg tcgaaactga tatttacacc aaaggatcgc
     1561 tcctggggct gttgctcgat atgcaggttc gacaccggac cgataatcag aaaacgctcg
     1621 acgatgttat gcgctatctc aatgccacct atgcccaaaa gcaagctggc tacccggaag
     1681 atggaattca aaaagccctt gaagctgtga ccggcagcag tttcgctgag ttctttgagt
     1741 cgtatgtggc tggcgtcaaa gaattgcctt ataaagaatt ccttgacctg gctggtgtcg
     1801 aagtcgtcga aggccggact attgataccc ctgaaagttt atttggagtg acatttggtg
     1861 gtgatgaaaa gcaggtcacg ttgactcagg tgagaccaga tgaagccggc tttcgggctg
     1921 ggctggctaa gggcgatatt ctggcggcta tcgaatttga acaggttacc caggcgtcgc
     1981 tcgc
//
LOCUS       DAOQDO010001285         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_56875_length_1984_cov_2.832556, whole genome shotgun sequence.
ACCESSION   DAOQDO010001285 DAOQDO010000000
VERSION     DAOQDO010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56875_length_1984_cov_2.832556"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(319..>1984)
                     /gene="acnA"
                     /locus_tag="PKZ53_18095"
     CDS             complement(319..>1984)
                     /gene="acnA"
                     /locus_tag="PKZ53_18095"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNJ42406.1"
                     /translation="PKQGECEYSVLLDLDLADIQPGVAGPKRPQDRINLTDLKTTFQG
                     LLEKPVKENGFNVAADRLSASVPVNVDAETEVTLGHGNVLIAAITSCTNTSNPSVMLA
                     AGILAKKAVEKGLTVNPAVKTSLAPGSRVVSEYLDKTGLQPYLNQVGFNVVGYGCTTC
                     IGNSGPLDQPIEDAVVKNDLVAASVLSGNRNFEARVHPNIKANFLMSPPLVVAFALAG
                     RVTTNLSEDPIGKGSDGADVYLRDIWPTSQEIGDLLSSAMDPETYQKLYSDFAEQNPM
                     WNDIPSSVGDVYEWDTESTYIQEPPYFENFGMDAGQFADVRGARPLAIFGDSVTTDHI
                     SPAGSIKASSPAGVYLQSKGVSVADFNSYGSRRGNHEVMMRGTFANVRIKNLMVPGVE
                     GGVTVYQPEGERLAIYDAAVKYMAAGTPLVVIAGQEYGTGSSRDWAAKGTRLLGVKAV
                     VAQSFERIHRSNLVGMGVLPCQFKDGTNAQSLQLDGTETFDLIGLEGGVKPQQDVTLV
                     IHRASGESTEVPLTLRIDTPIEIDYYLHGGILPYVLRQLITNADQN"
BASE COUNT          475 a          537 c          490 g          482 t
ORIGIN      
        1 gccctccagc aatacaatct cgattaaaaa ttggggaaaa ataagtaata ctattctgca
       61 atgaaaccct tgtattagaa aacagtcaag taattcaatc agatggagtt agcgaactac
      121 tgactatggg ctactgacta caaactggta ttatttcctc ctgatgattg gttctgaaat
      181 gtgctctgcg actggcaagt tacagccttg caaaggaaaa gggctgaaga aaccaggcca
      241 ggagttgagg attgccctca cctcttcagc ccgttttctt cagccccaga cccaaggtct
      301 tcagcccgat gttttgaatt agttctggtc agcgttggta atcaactgac gcagcacata
      361 cggcaaaatc ccaccgtgca ggtaataatc aatttcaatg ggcgtatcaa tccgaagcgt
      421 caacggaact tcggttgact caccactggc acgatggatg accagtgtga cgtcctgctg
      481 gggtttcacg ccaccttcca acccaatcag atcaaaggtt tcagtgccat cgagttgtaa
      541 tgattgggca ttggtgccgt ctttaaactg gcacggcagc acgcccattc caaccagatt
      601 gctccggtga atgcgttcaa agctttgagc cacgacggct ttgaccccaa gtaaccgggt
      661 gccttttgcc gcccagtcac gtgagcttcc ggtaccgtat tcctgtccgg caatcaccac
      721 aagcggtgtt ccggcggcca tatatttcac cgccgcatca taaatggcca gtcgttcgcc
      781 ttccggttga tagaccgtga cgccgccttc aaccccaggc accatcaggt ttttaattcg
      841 cacgttggca aatgtcccac gcatcatgac ttcgtggttt ccgcgccgtg agccatagct
      901 gttgaaatcg gcgacgctca cgcctttgga ttgcaggtag acaccggctg gcgatgaagc
      961 cttgatcgaa cccgccggac tgatgtgatc agtagtgacc gaatcaccga aaatggccag
     1021 tggtcgcgca ccgcgcacat cagcaaattg accggcgtcc ataccgaagt tttcgaaata
     1081 tggcggctct tgaatgtagg ttgattccgt gtcccattca tagacatcac cgaccgaact
     1141 tggaatgtca ttccacattg gattctgttc ggcaaagtct gaataaagct tctgataggt
     1201 ttcaggatcc atcgcgctgc tgagcaggtc gccgatttcc tgagacgttg gccagatgtc
     1261 ccgcaggtag acatccgcac catcgctgcc ttttccaatc ggatcttctg aaagattggt
     1321 cgtgacccgg cccgccagtg caaaagccac cacaagcggc ggactcatca ggaaattggc
     1381 tttgatgttg ggatgcacac gggcttcgaa gttccgatta ccggaaagga cgctggcggc
     1441 caccagatca tttttcacca cggcgtcttc aatgggctga tcgagcgggc cggagtttcc
     1501 aatacaggtc gtgcagccat atccaaccac gttaaaacca acctgattca ggtacggttg
     1561 caacccggtc ttgtccagat attccgaaac tacacgtgaa cctggggcca acgacgtttt
     1621 cacggctgga ttgaccgtca gtcctttttc aaccgctttt ttggccaaaa tgccagccgc
     1681 gagcatcaca ctggggtttg aagtgttggt gcaggaagta atagccgcaa tcaacacatt
     1741 gccgtggccc aaagtgacct cggtttcggc atccacgttg actggaactg acgccgacaa
     1801 ccgatcagcc gccacattaa agccattttc cttgactggt ttttccagca ggccctggaa
     1861 tgtggttttc agatccgtca ggttgatgcg gtcttgtggg cgtttgggac cggccacgcc
     1921 gggttgaata tccgccagat caaggtcaag caacactgaa tattcacatt cgccttgttt
     1981 cgga
//
LOCUS       DAOQDO010001286         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57105_length_1980_cov_3.529351, whole genome shotgun sequence.
ACCESSION   DAOQDO010001286 DAOQDO010000000
VERSION     DAOQDO010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57105_length_1980_cov_3.529351"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PKZ53_18100"
     CDS             <1..145
                     /locus_tag="PKZ53_18100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNJ42407.1"
                     /translation="GSRMTAMDSASKNATEVINKLTLNMNRIRQAAITNDIIEVVSGA
                     AAL"
     gene            364..1566
                     /locus_tag="PKZ53_18105"
     CDS             364..1566
                     /locus_tag="PKZ53_18105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HNJ42408.1"
                     /translation="MLVVICGGGPGGAVAAQKLAQSGVETILLEKNFTRIKPCGGAIP
                     PALIREFDIPEHIIERRVQAITVYAPSGQSVQLDTPNSFVGMVNREAFDQFLRDRATQ
                     AGALIVEARVETLDATELGAFVTFTTPDGRRKTVQPDCIIGADGVNSLVARKLNLVKG
                     GLQATAVQERFALKSDQIAQLIQRCEIYYDGRFSPDFYSWVYPKADHVVIGTATTVSN
                     ANVHIYLETFKQQLGIHDAPTLTETAQIPLRPLKSWTANRTVLIGDAAGLAAPTSGEG
                     IYYAMKSGVMAAEAILECSADLRAHKLEDAYQKRFMKEYGATYRQLEALQTTYYQSDE
                     HREALVHLCTDASIRDLAIQSYLFNKRLSPVSIFTSLKLKTKNAVQLTRVKLGHFTGN
                     EKLDKLAK"
     gene            1563..>1980
                     /locus_tag="PKZ53_18110"
     CDS             1563..>1980
                     /locus_tag="PKZ53_18110"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNJ42409.1"
                     /translation="MNQPISSKLHYISRVFHSVCVFVLVFVTVGAGPCGYKPAGKGGS
                     LPPNIRTIAIQTFTNESLRYRVEQRFTAALADEILRRGLPIKLTTDTTHADALITGSI
                     RNFGFRGVLVDDQGRIRLYELTITSAVTLRDQTSNRI"
BASE COUNT          482 a          534 c          490 g          474 t
ORIGIN      
        1 cggctcacgg atgaccgcca tggattctgc cagcaaaaac gccaccgaag tgatcaacaa
       61 actgacgctc aacatgaacc gcatccgtca ggcagcgatc accaacgaca tcatcgaagt
      121 cgtcagcggc gcggcggcac tgtaagacag ggttcagggt tcagagttca ggggttttcg
      181 aatttatgtc ctgttcgtct tttaagccct ttttgtcttt ttaacctgga acaggggcca
      241 cgaatcagtc taaactttct tattcggcgt ttggagcagc aaatatcggt tgctccaaac
      301 gccgaaatgt tttccaatgc cctggccggt tgacccctga ttctgaaacg acacgaaacc
      361 aatatgcttg ttgtgatttg tggtggtggc cctggtggcg cggttgccgc ccaaaaactg
      421 gcccaaagcg gcgttgaaac cattttactg gaaaaaaatt ttacccggat caaaccctgt
      481 ggcggggcaa tccctccagc actgatccgc gaattcgata ttccggaaca catcattgaa
      541 cggcgggttc aagccatcac cgtttatgca ccgtcaggcc aatctgttca gcttgataca
      601 ccaaacagtt ttgtcggcat ggtcaatcgc gaagcttttg atcagttctt gcgggatcgg
      661 gcgacccagg ctggcgcgct cattgtcgaa gcccgtgttg aaacgcttga tgcaacagaa
      721 cttggcgcct ttgtgacgtt tacaacgcct gacggcaggc ggaaaacagt gcaaccggat
      781 tgcatcattg gcgccgacgg cgtcaattcc ctggtcgccc gaaagctcaa cctggtcaaa
      841 ggcggtttgc aggcaacggc tgtccaggaa cggtttgcac tcaagtctga tcaaattgcc
      901 cagttgatcc agcggtgtga aatttattat gacgggcggt tttcacctga tttctatagc
      961 tgggtgtacc cgaaagctga ccacgtcgtg attggcaccg ccaccacagt gagtaacgcc
     1021 aacgtccata tctatctcga aacctttaaa caacaacttg gcattcatga cgcgccgacg
     1081 ttgactgaaa ccgctcaaat tccgctccgt ccattgaaaa gctggaccgc caaccggacg
     1141 gtgttgatcg gcgacgcggc tggactggcg gcaccgactt caggcgaagg tatttattat
     1201 gcaatgaaaa gtggtgtgat ggcggctgaa gccattttag aatgcagcgc tgatttgcgt
     1261 gcccataagc tggaagatgc ctaccagaaa cgatttatga aagagtatgg cgcaacttac
     1321 cgtcaacttg aagctttaca gaccacttac tatcagagcg atgaacatcg cgaagcactg
     1381 gtccatctgt gcactgatgc ctcaattcgc gatctggcca tccagtccta tctgttcaac
     1441 aaacgccttt caccagtctc gatttttacg tcattaaagc tcaaaaccaa aaacgctgtt
     1501 cagttgacca gggtgaaact tggtcacttt accggcaatg aaaagctgga caaacttgcc
     1561 aaatgaatca acctatctcg tcgaagctcc actatatatc gcgtgtgttt cacagcgtct
     1621 gtgtatttgt tctggtgttt gtgacagtcg gtgctggccc ctgtggatat aaaccagccg
     1681 ggaaaggtgg ttcgttgcca cccaatatcc gcacgattgc cattcagaca tttaccaacg
     1741 agagcctgcg ctatcgcgtc gaacaacgct ttacggcggc tctggcagat gaaattctgc
     1801 gccggggctt gcccatcaag ctcacaactg acaccacgca tgccgatgcg ctcatcaccg
     1861 ggagcatccg taactttggg tttcgagggg tactggttga cgatcagggc cgcatccgcc
     1921 tctatgaact caccatcact tcggctgtaa cgctgcgcga ccagacctcc aatcgcattc
//
LOCUS       DAOQDO010001287         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57109_length_1980_cov_3.412987, whole genome shotgun sequence.
ACCESSION   DAOQDO010001287 DAOQDO010000000
VERSION     DAOQDO010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57109_length_1980_cov_3.412987"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..821
                     /gene="lexA"
                     /locus_tag="PKZ53_18115"
     CDS             210..821
                     /gene="lexA"
                     /locus_tag="PKZ53_18115"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNJ42410.1"
                     /translation="MSIPKTDLPLGRKIYEIRQASHLTQTQLARKARLTQGMIAQIEN
                     GKRSVSDRTLIVLAQTLGNDFGEEWLTPFVSASSSVVNVEGAIAAGKPIEPISQHLEL
                     AIPTSMLRRGREHFALRVQGDSMIEEHICDGDVVIAVRTPTAENGQVVVALVDGNATL
                     KKFYRQKNTIVLQPANPNHPPMVFDKKTNVEVQGVVVGIVRNL"
     gene            1334..>1980
                     /locus_tag="PKZ53_18120"
     CDS             1334..>1980
                     /locus_tag="PKZ53_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNJ42411.1"
                     /translation="MNFIAFLFQQTGHYLFHGACALALVLMCSTSTLADEKRGTLSGR
                     VSDAAGGALVGATVTLRQAAVGFEVSVQTDSTGSFQFSELLAGDYTLSASARDYALNS
                     KPVTISDGAIATLDLTLQPGTFSEDITVVSGSIAGVPEILQRIPGTVDILEPKTLQQS
                     RLFNFNEALRKVSGINVRDEEGFGLRPNIGIRGLNPTRSTKVLLLEDGIPLAYAP"
BASE COUNT          485 a          462 c          509 g          524 t
ORIGIN      
        1 gatggccctg gctgcatttc gaacgacgag gtggggggta atggtgtgaa aaccatccgg
       61 gattggttta acttgggttg acatggagtt ctcctttagg gtcaaaaaaa attgtgtgga
      121 agcctggatt ttttgtttta cgaattgaaa aattcgtggc aatatgcagt tggtagataa
      181 cgcggtaatt attactcggt gataaaaata tgtcaatacc aaaaactgat cttccacttg
      241 gtcgtaaaat ctacgaaatc cgtcaggcaa gtcacctcac tcaaacccaa ctggccagga
      301 aagcccggtt gactcaaggc atgatcgccc aaattgaaaa tgggaagcgt tctgtttctg
      361 atcggacctt gattgtcctg gcacaaaccc tgggcaatga ctttggtgag gaatggctca
      421 ccccatttgt gtcggcatcg tcttcagtgg tgaatgttga aggggcgatt gcggcaggca
      481 aacctatcga accaatttcc cagcacctgg agctagctat tccgacctca atgcttcggc
      541 gtggacgcga gcactttgcc ttgcgagtcc agggtgattc gatgatcgaa gagcacatct
      601 gtgatggcga tgtggtgatt gccgtcagaa ccccaacggc tgaaaatggg caggtcgtgg
      661 tggcgctggt tgatggaaat gccaccctca agaagtttta ccggcaaaaa aatacgattg
      721 tgctccaacc agccaaccca aatcatccgc cgatggtttt tgataaaaaa accaatgttg
      781 aggttcaagg ggtggtggtt ggaatcgttc ggaatctcta atttcaactg aactcaatca
      841 aagtgttgat catttccagg aaatgggcca ggtggcccat tttttgtttt gagccatcaa
      901 cttttatgct ccaggtgtca aaaagcgtgg cctgaaagaa ggtggaggct cccgttggcc
      961 accaagaaat tgcttgacag gctaccctgc ctaagataga agttgaatat cattttcgat
     1021 atgaaccttc acgacagatc ccttttcctc taagtcctgc cagttttttc ttcccaagca
     1081 caggccaggt gacttttttg gtgagttcca ggtgatttgg ttccttgaag caggtggtcg
     1141 caatctcctt tccttgtaac tccggagcgt tgctttttcc acaactgctc tgacagtttt
     1201 gaggttgatt gctggtgaca cacaaactgt tattcagaaa tttcagaaaa tgacctgcgc
     1261 gttcaactca aaaattagaa gcaagcgata tcacttcacc acgtgtagcg gttgatagtg
     1321 caaggagtaa tccatgaatt tcatagcttt tttatttcaa cagaccgggc attatttatt
     1381 tcacggggct tgtgccctgg cactggttct gatgtgttca acctcgaccc tggccgatga
     1441 aaagcgcgga accctttccg gtcgagtgag cgatgcggcg ggcggtgcac tggttggtgc
     1501 cacggtgacg ctccggcaag ccgcagtcgg ttttgaagtt tcagtccaga ccgatagcac
     1561 cgggagcttt caattttctg agcttctggc cggtgactat accctcagtg ccagtgcccg
     1621 tgactatgca ctgaattcaa agcccgtgac catcagtgac ggggcaatcg caaccctgga
     1681 tctgaccctg caaccaggta cgttctctga agatattacg gtggtgagcg gttcaattgc
     1741 tggtgtacct gaaatcctgc agcgaattcc cggtacggtt gatattttgg agccgaaaac
     1801 ccttcagcaa agccggctct ttaatttcaa cgaagccctc cgcaaggtgt cgggcatcaa
     1861 tgttcgcgat gaagaagggt ttgggttgcg gcccaacatt ggcatccggg ggttaaatcc
     1921 aacgcgctca accaaagttc tgttgctcga agacggtatc ccgctggcct atgcgccata
//
LOCUS       DAOQDO010001288         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57169_length_1979_cov_2.651767, whole genome shotgun sequence.
ACCESSION   DAOQDO010001288 DAOQDO010000000
VERSION     DAOQDO010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57169_length_1979_cov_2.651767"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKZ53_18125"
     CDS             <1..555
                     /locus_tag="PKZ53_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNJ42412.1"
                     /translation="GATVGKMLGFHRAMKGGIGAAAIHTADGLCVAALIAANPWGDIR
                     DPRTGHILAGTRGEDGKTLVDPIRLMREGNLLDPFTPASSSNTTIGLVATNAALTKTE
                     LTKVAQMAHDGLARTIFPIHTPFDGDTIFAVAVPAYIKGQPFVTHPGRVGILAAEVVA
                     DAVIRAVQTAVGLPGIPGAAELEG"
     gene            578..1141
                     /locus_tag="PKZ53_18130"
     CDS             578..1141
                     /locus_tag="PKZ53_18130"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ42413.1"
                     /translation="MKKFVFRLCFLSVVVCVATGTTFAIQSQKAKPNPERIPIPVEVL
                     PVFDIPRPWLSWTFVDPEVFPEHFKPSQPPSSAVCYGSSPPTVRSTNSPAFCGERGIR
                     PQIIRQSKARYTDEARKALIVGKVVLAVEFLTDGTIGEIRVVRSLGYGLDEQAVEAAR
                     QIKFRPAMKDGEAIPFTHRVEFSFQLP"
     gene            1324..1884
                     /locus_tag="PKZ53_18135"
     CDS             1324..1884
                     /locus_tag="PKZ53_18135"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ42414.1"
                     /translation="MKKLVFRLCLLSVVMCVATNSIPAIQSQKAKPNPERIPIGPEPA
                     QMLNAPGIELAVDFREVDWALKSFDITNRGISGCQPNSLAYSHGYRSPDPNIPDFRHR
                     IISQPKPHYTDEARKAHIVGKVVLSVEFRADGTIGNIRLLRSLGYGLDEEAIVAARQI
                     KFHPALKNEKPMTYTSRVEFSFNLID"
BASE COUNT          513 a          496 c          473 g          497 t
ORIGIN      
        1 ggtgccacgg tcggaaaaat gctcggattt catcgggcca tgaaaggcgg cattggcgcc
       61 gccgccattc ataccgcaga tgggctctgt gtcgccgccc tcatcgcggc caacccctgg
      121 ggagacattc gcgaccctcg caccggtcac atcctggctg gaacacgcgg cgaagatgga
      181 aaaaccctgg tagatccaat ccggctgatg cgtgaaggca atctgcttga cccgttcacc
      241 ccggcctcaa gttcaaatac gactatcggc ctggtggcaa ccaacgccgc cttaaccaaa
      301 accgaactga ccaaagtggc gcaaatggcc cacgatggtc tggcacgcac catttttcca
      361 attcataccc catttgacgg agacaccatt tttgccgtcg ccgtgcctgc atatataaaa
      421 ggacaaccat ttgtaactca tccggggaga gtcggcattt tagcggcaga ggtcgtggcc
      481 gatgcggtga ttcgcgccgt gcaaactgcc gtgggattgc ctggaattcc aggcgcggct
      541 gaacttgagg gttgaacttt tttccaatcg ggctccaatg aaaaaatttg tcttccgact
      601 gtgttttctg tcagtggtgg tgtgtgttgc gacaggcact accttcgcaa tccaatccca
      661 aaaagctaaa cccaatccag aacgaattcc gattccagtt gaagtacttc cggtttttga
      721 tattccacgg ccctggcttt cgtggacgtt tgttgatcct gaagtatttc cagagcattt
      781 caaaccttca cagccacctt caagtgcagt gtgttacggt tcttcacctc caacagtccg
      841 atccacaaac tcaccagctt tctgtggtga acgtggaatt cgaccccaaa ttatcagaca
      901 gtcaaaagcc agatataccg atgaagcaag aaaagcgctt attgttggga aagtggtact
      961 tgcagttgaa ttccttacgg acgggacaat tggtgaaatt cgggttgtca ggtcactcgg
     1021 ctatggactg gatgagcagg cggttgaggc ggctcgtcaa atcaaatttc gtccggctat
     1081 gaaagacgga gaggcaattc cctttaccca tcgggttgaa ttttcatttc agcttccatg
     1141 aattaagacc atccttctga ccaactggca aaactaaaga cctgtttatt tagatttgga
     1201 taactggcca taaaataacg atactggctg taatgcattc gggaaaagcc ggcattctaa
     1261 caacaacggc accagcctga gatctgaaaa tagattccct tctctttttc cggagcatct
     1321 tcaatgaaaa aacttgtctt ccgactgtgt ttgctgtcag tggtgatgtg tgttgcgaca
     1381 aactctatcc ctgccatcca atcccaaaaa gccaaaccga atccagaacg aattccaatc
     1441 ggacctgagc cggcacagat gttaaacgcc cctggtatag aacttgcagt tgattttcgt
     1501 gaggttgatt gggctctaaa gtcatttgac atcacgaatc ggggaattag cgggtgtcag
     1561 ccaaactctc tagcctattc ccatggatac cggtctccgg acccgaatat acctgatttt
     1621 cggcatagaa ttatcagtca gccgaaaccc cattacaccg atgaagcgag aaaggcgcat
     1681 attgtcggga aagtagtcct ttccgttgag ttccgtgccg atggaacaat tgggaatatt
     1741 cggttactca ggtcgctggg ctacggattg gatgaggagg cgattgtggc agctcgtcag
     1801 atcaaatttc atccagccct gaaaaacgaa aagccaatga catataccag tcgggttgaa
     1861 ttcagtttta acctgattga ttgagtcaat ttgtttgggc tgagcaggaa ttaaccggca
     1921 gaaaatctgc acactgaact ttttccaacc gtgttcgaat gaaaaaattt gtcttccac
//
LOCUS       DAOQDO010001289         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57184_length_1979_cov_2.326923, whole genome shotgun sequence.
ACCESSION   DAOQDO010001289 DAOQDO010000000
VERSION     DAOQDO010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57184_length_1979_cov_2.326923"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PKZ53_18140"
     CDS             complement(<1..510)
                     /locus_tag="PKZ53_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ42415.1"
                     /translation="MPHELPLIRDLLVLLTSSIVVSLICFRIRVPMLIGFMLTGILIG
                     PHGLGLIRDVHAVEILAEIGVVLLLFTVGLEFSLKSLFEMRKIVVNGGGLQVVATTAV
                     VTLIFWMAGKPLNQGIFYGFLCTLSSTALVLKTYADRAETDTPHGRIGLGVLLFQDLC
                     VVPMMLLVPI"
     gene            complement(510..686)
                     /locus_tag="PKZ53_18145"
     CDS             complement(510..686)
                     /locus_tag="PKZ53_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42416.1"
                     /translation="MNFYFLVWLLNALLERTPLHQTLQSCTACGRTALYHKDRGNTYV
                     CRHCHFVFKGKEKR"
     gene            complement(758..1240)
                     /locus_tag="PKZ53_18150"
     CDS             complement(758..1240)
                     /locus_tag="PKZ53_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42417.1"
                     /translation="MLTSFCYQVVCGIALCLLGLSVGVGSYPPQISSSNQTQSTPGWL
                     VSTPPVLPLVKNFRQPTLQRSHPLPVNACWPATSDERNVSTTSSNQTFPNVVDVRVSP
                     TCDQSSTSPQSKTIGLIPAPAFTVVVKTLNLLLVNFSSSKYQLLLFGSSFAPRAPPCW
                     "
     gene            complement(1405..>1979)
                     /locus_tag="PKZ53_18155"
     CDS             complement(1405..>1979)
                     /locus_tag="PKZ53_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42418.1"
                     /translation="TRRHRFLSTFLWELPFGKYPRDFSPVLNALVGGWQVSGITLFQS
                     GAFLTPTFSGTDPSGTGVLVRGVTTTQRPDRIGNGNLSNPTREQYFDPSAFVIPGNNI
                     GRFGNAGVGILQGPGTKVFSLSVAKKFNLTERVFVRYEATISNLLNHTNLDIPSSLNV
                     SSPGFGRITSTQSVDLAGPRTVQMSLRVGF"
BASE COUNT          551 a          500 c          536 g          392 t
ORIGIN      
        1 aatcggcacc agcaacatca tcggcaccac gcataaatct tgaaagagca gcacaccaag
       61 gccgatgcgt ccgtgaggcg tgtcggtttc agcccggtcc gcataggttt ttaacaccag
      121 cgcggtgctc gacagcgtgc ataaaaatcc gtagaaaatg ccttgattca acggtttgcc
      181 ggccatccag aaaataaggg tcaccacggc tgttgtggcc acaacctgga ggccaccacc
      241 gttcacaaca atcttgcgca tttcaaacag cgacttcagg gaaaattcaa ggccaacggt
      301 aaaaagcagc aacaccaccc cgatttcagc caggatttcg acggcgtgga catcgcgaat
      361 cagcccgagg ccgtgcggcc cgatcagaat tccggtcaac ataaaaccaa tcagcatcgg
      421 aacccgaatg cgaaaacaga tcaaggaaac cacaatcgaa gaggtcaaca acaccagcaa
      481 atcgcgaatc aatggaagtt catgaggcat cagcgttttt cctttccttt aaagacaaag
      541 tggcaatgac ggcagacata ggtattgcct cgatctttgt gatagagggc cgtccgacca
      601 caggctgtgc aggattgaag cgtctgatga agcggggtgc gttcaagcaa ggcattgagc
      661 agccagacca gaaagtaaaa attcatacac cagtcctctc aaggcagaca cttgtggatc
      721 gcagcggctt aacgccgctt aagagatgca gtgtgaacta ccagcagggc ggtgctcggg
      781 gagcgaatga acttccaaaa agaagaagct ggtattttga cgacgaaaaa ttcaccaaca
      841 acaggttgag cgtttttacg acaaccgtga aggctggtgc cggaatgagg ccgatggttt
      901 tggactgggg tgatgtcgaa gactgatcac aggtcgggga aacccgcaca tcaacgacat
      961 tggggaatgt ttgatttgaa gaagtcgtgg agacgtttcg ttcatcactg gtcgccggcc
     1021 aacaggcatt gactggcaaa gggtgtgacc gttgtagggt cggttggcgg aaatttttca
     1081 ccaacggcaa gactggcggc gttgacacca accaacctgg tgtggattga gtctgatttg
     1141 atgaactgat ctgtggtgga tatgaaccaa cgccgacact gagaccaagc aggcagaggg
     1201 caatgccgca aacgacttga tagcaaaaac ttgtcagcat cggtctgtac cccctccttt
     1261 cgtggtgtgg gctgtgggat tcaggatctc aaaggacgaa agggacaaaa aggacaaaaa
     1321 ggacaaaagt tcgaaaaccc tgaacccgga accctgaacc cggaaccctg aaccctgaac
     1381 ccggaacccg gaacccgatc aacctcaaaa cccaacccga agtgacattt gtaccgttcg
     1441 agggccagcc aggtcaacgc tttgggttga ggtaatgcgt ccaaaacctg gcgatgaaac
     1501 attgagcgat gagggaatgt cgagattggt gtgattcaac aggttggaaa tcgtggcttc
     1561 gtagcgcaca aacacccgtt ccgtcaggtt gaattttttg gccaccgaga gcgaaaagac
     1621 tttggtgccc gggccctgga ggatgccgac tccggcgttt ccaaaacgcc cgatgttgtt
     1681 tcccggaata acaaaggcac tcgggtcaaa gtattgctca cgggttgggt ttgaaaggtt
     1741 gccatttcca atccggtctg gtcgctgggt tgtggttaca ccccggacga gaacccccgt
     1801 ccccgacgga tccgtgccgc taaatgttgg cgttagaaag gctccggatt gaaacagcgt
     1861 gattcctgat acctgccagc caccaaccag cgcattgaga actgggctga aatccctcgg
     1921 gtatttgcca aatggcaatt cccacaaaaa cgtgctcaaa aaccgatgcc gccgggtaa
//
LOCUS       DAOQDO010001290         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57316_length_1976_cov_3.340968, whole genome shotgun sequence.
ACCESSION   DAOQDO010001290 DAOQDO010000000
VERSION     DAOQDO010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57316_length_1976_cov_3.340968"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="mraY"
                     /locus_tag="PKZ53_18160"
     CDS             complement(<1..990)
                     /gene="mraY"
                     /locus_tag="PKZ53_18160"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNJ42419.1"
                     /translation="MIPDIRNAKGWFFVLYYLLYHVLRELHPSFGALRVFGEVSFRAA
                     LAAITALIISLAFGPLMIEKLRILKFGQEIREEGMTTHRIKRGTPTMGGILIITAVAS
                     ATLLWANLSNLYVWVVLFALLSHGAIGFVDDFLKIKKKHNMGLQGRYKLIGQFGSEII
                     IGLVLLFGAHYSTQLSVPFFKDFRPELGWVLYLAFMIIVMTGSSNAVNLTDGLDGLAI
                     STTFVVASALAGLTFVTGYADAAKYLGLTANPEAWEVTVFCAALAGASLGFLWFNAPP
                     AEVFMGDVGSLAIGGAIGCIAILIKQELLLVVIGGIFVIEALSVIMQTTYYKMT"
     gene            complement(1032..>1976)
                     /gene="murF"
                     /locus_tag="PKZ53_18165"
     CDS             complement(1032..>1976)
                     /gene="murF"
                     /locus_tag="PKZ53_18165"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNJ42420.1"
                     /translation="GMSFSGEITQLTQIAPPHISVVLNVAPVHLENFDSIDGIAAAKA
                     EIVHGLIEGGTAILNVDDPRVRQMDEIARAKNGKVVFFGRGENAQVRAIDVTPHGLLG
                     TDFTLVTPKGTIPVHLPLPGEHFVHNALAAAAVALECGGYLDSIAAALEQGKPAPHRG
                     EVLHFEAGFTVVDDSYNSNPQALQGMIKLLAAVPDARRTVLVAGEMLELGPDGAQMHA
                     ACGKFAAEHGVGLLIGVRGLAEHFLTGAYTAGLPHSQTYFAPDATQAGEWLAQAVRPG
                     DVVLVKGSRGVRTELVIETLKHRFSVCPTTPTQTGLYQ"
BASE COUNT          499 a          560 c          498 g          419 t
ORIGIN      
        1 ggtcatcttg taataggttg tttgcataat gaccgacagg gcctcgatga caaaaatccc
       61 gccaatgacg accagcaaca gctcttgttt gatcaaaatg gcgatacagc caattgctcc
      121 gccaatcgcc aggctcccaa catcacccat aaagacttcg gctggtggtg cgttaaacca
      181 caaaaaccca aggctggcac cagccagagc ggcacaaaaa acggtgactt cccaggcttc
      241 cgggttggct gtcagcccaa ggtactttgc cgcatccgca tacccggtga caaacgtcag
      301 gccagccagg gccgaagcca ccacaaaggt ggtgctaatg gccagtccat caagtccgtc
      361 ggtcaggttc acggcgttag aactgcccgt catcacgata atcataaagg ccaggtacag
      421 cacccagccg agttccgggc gaaaatcctt aaaaaatggc acgctgagct gggttgagta
      481 atgcgcccca aagagcagca ccagtccaat aatgatttca gacccaaact gaccgatcag
      541 tttatagcgt ccctgaagcc ccatgttgtg cttcttttta attttcaaaa aatcatccac
      601 aaagccaatt gccccgtggg aaagcaatgc aaacagcacc acccagacat acagattgct
      661 gagattggcc cagagcagtg tggcgcttgc caccgccgta atgatcagga tgccacccat
      721 cgtcggcgtt ccgcgcttga tgcggtgagt ggtcatgcct tcttcacgga tttcctgtcc
      781 aaattttaag atgcgcagct tttcgatcat caatgggcca aatgcgagac tgatgatcag
      841 tgccgtgatt gctgcgagcg cagcccggaa cgaaacttcg ccaaacacgc gaagcgcgcc
      901 aaagctggga tgaagttctc gtaaaacgtg gtagagcagg taatagagca caaaaaacca
      961 gcctttagcg tttcgaatat ctggaatcaa gcagcatcat tttggtaaac ctcctcagtc
     1021 cgacacctgt tttattggta caacccggtt tgagtgggtg tggtcggaca aaccgaaaag
     1081 cggtgtttga gcgtttcaat gaccagttcc gttcgaacgc cacgagatcc tttgaccagc
     1141 accacatctc cagggcgaac ggcttgcgca agccattcac cggcctgggt ggcgtcaggt
     1201 gcaaaatatg tttgagaatg gggcaacccg gcggtgtatg ccccggtcag aaaatgttct
     1261 gccagcccac gaaccccaat tagaagtcca actccgtgtt ctgccgcaaa ttttccgcac
     1321 gcggcatgca tttgggcgcc gtctggtcca agttccagca tttccccagc gaccagcacg
     1381 gtgcggcggg catcgggtac ggcagccagc agcttgatca ttccctggag tgcctgcggg
     1441 ttggagttgt aggaatcatc cacgaccgta aagccagctt caaaatggag aacttcaccg
     1501 cgatgcgggg ccggttttcc ttgttcaagc gctgcggcaa tactgtccag atagcctccg
     1561 cattccaatg ccactgccgc cgcggccagt gcattgtgga caaaatgctc gcccggtaac
     1621 ggcagatgaa ccggaatcgt gcctttcgga gtgaccagcg taaaatctgt tccaagcaat
     1681 ccgtgcgggg tgacatcaat ggcacggacc tgagcgttct ccccacgacc aaaaaacaca
     1741 accttgccat ttttcgcccg agcgatttca tccatctgac gaacccgagg atcatcaaca
     1801 ttgagaatcg ccgttccacc ttcgatcaag ccgtgaacaa tttcagcttt ggccgctgca
     1861 attccgtcaa tgctgtcaaa gttttccaga tgcaccggcg ccacgttgag caccacactg
     1921 atatgaggcg gggcaatctg ggtcaattgg gtgatttcgc cagaaaagct catgcc
//
LOCUS       DAOQDO010001291         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57360_length_1975_cov_6.947917, whole genome shotgun sequence.
ACCESSION   DAOQDO010001291 DAOQDO010000000
VERSION     DAOQDO010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57360_length_1975_cov_6.947917"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1407)
                     /locus_tag="PKZ53_18170"
     CDS             complement(49..1407)
                     /locus_tag="PKZ53_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNJ42421.1"
                     /translation="MGFTLRFDRGTLLLEGVTDLPASVTGLFRFDPRVDAFRASCEQY
                     REIVEKLGPDLEANRAPRYQRLKLSPLLSFEPYPHQTAALQAWTKAGCRGLVVLPTGA
                     GKSLIGMQALASCGRSGMVIVPTIDLMHQWFALLKAAFPETEVGLLGGGYHDASPLLV
                     STYDSAARHMERLGNQYGVLIADEVHHLPSEFYRSIAEFSLAPYRLGLTATLERSDMR
                     HRDLFSLIGPLVYHCDPEDLAGDVLAPYQIKRLYVELTSTEREAYTAAQRQRDAFLEA
                     NNISLKQLDGWSRFVMISSRTIEGRQAMHAHQELRRLSHAAPAKLRALELILAEHPQE
                     KAVVFTEDNATVYELSKKFLIPCITHQTPIKERHHILTQFRNGTYRTLATSKALNEGV
                     DVPDASVGVILSGSAVRREFVQRLGRILRRAEGKKAVLYEVVTRQTREEGVAARRMRN
                     EE"
     gene            complement(1471..>1975)
                     /locus_tag="PKZ53_18175"
     CDS             complement(1471..>1975)
                     /locus_tag="PKZ53_18175"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HNJ42422.1"
                     /translation="GWFACVVGAAKGYVWLGPLFVVLFLIGHILLVAPDRLREVKFIL
                     AVGVLGAVADSLQKMYGVLAYMDDFVRLQWLAPAWIISLWVLFASSFSTSLEWMHRRY
                     LVGAVFGAIGGPLSYLAGTKFGAITFKYDPTFSMLVVGLVWGSIMPILLVMAEKFGLV
                     QPVKIET"
BASE COUNT          509 a          531 c          494 g          441 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattcttc attcttcatt
       61 cctcattcgc ctggcagcga caccttcttc tcgggtttgg cgggtgacga cttcatagag
      121 cacggctttt ttgccttcag cgcgtcgcaa aatgcgtccg agacgttgga caaactctcg
      181 acgaacagca ctgcctgata aaatgacccc gactgaggca tctggtacgt ctacaccttc
      241 atttaaagct ttgctggtgg ccagggtgcg ataggttcca tttcgaaatt gggtcaaaat
      301 atggtgacgc tctttaattg gtgtctggtg ggtaatacag ggaatcagga atttcttgga
      361 aagttcatag acggtggcgt tgtcttcggt aaaaacgacg gctttttcct gtggatgttc
      421 agccagaatc aattccagcg ctctcagttt ggctggtgcc gcgtgcgaga gtcgtcgaag
      481 ttcctgatga gcatgcattg cctggcggcc ctcgatggtt cgtgaagaaa tcatgacaaa
      541 ccgactccat ccatcaagtt gtttgagtga gatgttgttg gcctcaagga aggcatcgcg
      601 ctgtcgctgg gctgccgtgt acgcctcccg ttcagtactg gtgagttcga catacaatcg
      661 tttgatctgg tacggcgcca gtacatctcc ggccaggtcc tcggggtcac agtgatagac
      721 cagcggtcca atcaggctga ataaatcccg gtggcgcata tcactgcgtt ccagcgtcgc
      781 agtcagcccc agccgatagg gtgccagtga aaattcagca atgcttcggt aaaactcaga
      841 gggaagatga tgaacttcgt ctgcaatcag cacaccatac tgatttccaa gtcgttccat
      901 gtggcgggcg gctgaatcat aggttgacac caacaacggg ctggcatcgt gatagccgcc
      961 tccgagcaat ccaacttcgg tttccgggaa ggccgctttg agcaaggcaa accactggtg
     1021 catcaggtca atggtcggaa caatcaccat gccgctacgg ccacagcttg ccagggcctg
     1081 cattccaatc aggctttttc cggcaccggt tgggagcacc accagcccac gacaaccagc
     1141 tttcgtccag gcctggaggg ccgcagtttg atgggggtat ggttcaaatg aaagtaaggg
     1201 cgagagtttt aaccgctggt accgcggcgc ccggttcgcc tccaggtccg gccccaattt
     1261 ttcgacaatt tcccgatatt gttcacagga agctcgaaac gcatcaaccc gtgggtcaaa
     1321 gcgaaacaga cctgtcaccg acgctggaag gtcggtgact ccctcaagca ataatgttcc
     1381 tcgatcaaac cgaagtgtaa aacccatgcc agaccaatga agaatgaaga atgaagaatg
     1441 aagaatgaag aataaaaacc cagtaaatcg ttacgtctca atctttacag gctggacaag
     1501 cccaaatttc tcagccatga ccagcaaaat tggcataatg gaaccccaga ccaggccgac
     1561 gacaagcatt gaaaaagtgg gatcatattt aaaggtaatg gcgccaaatt tggtgcccgc
     1621 cagatagctc aacggaccac caatcgcgcc aaacactgcc ccgacgagat agcgccggtg
     1681 catccattca agcgaagtac taaaactgct ggcaaagagt acccagagcg agataatcca
     1741 ggctggggcg agccattgca gtcgcacaaa atcatccata taggccagca cgccatacat
     1801 tttttgcagg ctatcagcca cggctcctaa cacaccgacg gcaaggataa atttcacttc
     1861 acgcaggcgg tcaggtgcca ccagcaggat gtgtccgatc aaaaaaagaa ccacaaacag
     1921 tggaccaagc cagacatatc ccttggcggc gccaaccacg cacgcaaacc agccg
//
LOCUS       DAOQDO010001292         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57390_length_1975_cov_2.406250, whole genome shotgun sequence.
ACCESSION   DAOQDO010001292 DAOQDO010000000
VERSION     DAOQDO010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57390_length_1975_cov_2.406250"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1975
                     /locus_tag="PKZ53_18180"
     CDS             <1..>1975
                     /locus_tag="PKZ53_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ42423.1"
                     /translation="LSPQPQAPSPRYYRTGDRVRWLPDGNLEFLGRLDTQVKLRGYRI
                     ETDEIEAVLQRHPLVREAVVVVRRDSTDDQRLVAYVTLSADDSRPEMAYPAQLVAFLQ
                     PHLPEFMLPSAVVVLPELPRLPNGKTDRRALPAPDQSLFGTNRSVDLPRTPLEEMLIE
                     LWKQVLKVEAIGIHDNFFALGGHSLLATQLITRLRKTFEIELPLKRIFEAQTVAQLAA
                     IITQAQAQSVQLATGQPRPQLVAVSRNRPLPLSFAQERLWFLDQLKPDSPLYNIPLVF
                     ALPETVHLSELEQAFTEMIRRHEILRTTFPSFEGEPVQVIGSPVDFHFEEHFIPDSET
                     ADERQITAFLRQPFNLERGPLLRGAIFRSATQKTIVAVSVHHIVFDGWSSGVLFRELA
                     QIYRALHQPQSPGLPELPIQYADYAVWQRNWFQGAVFESELAFWKNQLAGIPPLLELP
                     TDRPRPVIRSFQGARQFFEFDESATEKLKTLSRQENVTLFMVLLTAYVVLLARYSGQN
                     DISVGTPVANRTQAETEALIGFFVNTLVIRTQLEAEPTFLEVLRQIKHTVLDVFAHQE
                     MPFEKLVEVLQPERNLSHTPLFQAFFVFQAASPAEFKPFGESAPVSLRSAQSQPAAED
                     QRVAPGMAKFDLTLSIFEKGRHIRHDVPRYIQAG"
BASE COUNT          443 a          550 c          494 g          488 t
ORIGIN      
        1 ccctcagccc ccagccccaa gccccaagcc cgaggtatta tcgtaccggt gaccgtgtcc
       61 gctggcttcc ggatgggaac ctggagtttc ttgggcggtt ggatacccag gtcaaactgc
      121 gcggctatcg gattgaaact gatgaaattg aagccgtttt gcagcgacat ccgctggtgc
      181 gtgaagcggt tgtagtagtg cggcgcgatt cgaccgacga tcagcgactg gtggcctacg
      241 tgacgctttc ggcggatgat tcccgcccgg aaatggcgta tccggcacaa ctggtggcct
      301 tccttcagcc tcatctgcct gaatttatgc tgccatccgc cgtggtggtc ctgcctgaat
      361 tgccccggct tccaaatgga aaaactgacc gccgggcact tcccgcaccc gaccagagcc
      421 tatttggaac gaatcgaagc gtggatttgc cccgaacgcc ccttgaagaa atgctcatcg
      481 agctttggaa acaggtgctc aaagttgagg caattggcat tcacgacaat ttctttgcac
      541 tgggcgggca ttccctgctc gcaacgcagt taatcacccg acttcggaaa acctttgaaa
      601 ttgaacttcc actcaaacgg atttttgaag cccaaaccgt ggcgcaactg gcagccatca
      661 tcacccaggc gcaagctcaa tcagttcaac tagctacggg tcaaccccgt ccgcaactgg
      721 tggcggtttc ccgaaatcgt ccgctcccgc tgtcatttgc ccaggaacgg ctctggtttc
      781 ttgatcagct caaaccagac agtccgctct ataacattcc actggtcttt gccctgccgg
      841 aaactgttca tttatctgaa cttgaacagg catttacgga aatgattcgc cgtcatgaaa
      901 tattgcgaac aacctttccg tcatttgaag gtgagccggt tcaggtgatt ggctcgccgg
      961 tggatttcca ctttgaagaa cacttcattc cagattctga aactgctgat gaacggcaga
     1021 taacggcctt tttacgtcag ccattcaatc tcgaacgtgg accgttgctg cgtggtgcga
     1081 tttttcgaag tgccactcaa aaaacgatag tggctgtgtc tgtccatcac attgtgtttg
     1141 atgggtggtc ttcgggtgtg ctttttcgag aactcgccca gatttatcgt gcactgcatc
     1201 agcctcaatc accaggtttg ccagagctgc cgattcaata tgccgattat gccgtctggc
     1261 agcgcaactg gttccaggga gctgtttttg aaagcgaact ggcattttgg aaaaaccagc
     1321 ttgcgggtat tccgccgctg cttgagcttc caaccgaccg accccgaccg gttattcgct
     1381 cgtttcaagg cgcccgccag ttttttgaat ttgatgaatc agctaccgaa aagctgaaaa
     1441 cactgagtcg tcaggaaaat gtcaccctgt ttatggtgct cctgaccgcc tatgtcgtgc
     1501 tgctggcacg gtattcaggt caaaatgaca tcagtgttgg aacgccagtt gccaatcgga
     1561 cccaggcgga aaccgaagcc ttgatcgggt ttttcgtcaa cacactggtc attcgaaccc
     1621 aactggaagc tgaaccaacc tttctggagg tccttcgaca gatcaaacat accgtccttg
     1681 atgtgtttgc ccatcaggaa atgccgtttg agaaactggt tgaagtgctg caaccggaac
     1741 gaaatctcag ccacacgcca ctgtttcaag ccttttttgt ttttcaggcc gcttcaccgg
     1801 ctgaattcaa accgtttggc gagtcagctc cggtttcact ccggtctgct cagtcccaac
     1861 cggcggcaga ggatcagcgc gtggcacctg gaatggcaaa atttgacctc accctgtcaa
     1921 tttttgaaaa agggcgccac atacggcatg acgtaccacg gtacatccag gccgg
//
LOCUS       DAOQDO010001293         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57551_length_1972_cov_2.710485, whole genome shotgun sequence.
ACCESSION   DAOQDO010001293 DAOQDO010000000
VERSION     DAOQDO010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57551_length_1972_cov_2.710485"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKZ53_18185"
     CDS             <1..949
                     /locus_tag="PKZ53_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42424.1"
                     /translation="RDVVPMGLFPGTTKILYDVLFQRTRGAKEPTRFMQTTSAALVKW
                     ARTSEKTLWRHLSYLERVGLVRRRTDLIQDIKFKKNGGNIYEIFVPEEIDLPFALTIA
                     EESRLTTQSIHSFPQGNRREGNSTVRTKVPGTEGNNVPYTTKTNPLKSNISEPLRHDN
                     TRYNTEEESAASRLAQYLFLTFEEYLGRPLATEEKLAIFATLSEELLDLIHQGLNANA
                     EHGQKQIENPVAWFTAMIGQRLTGRNRKRSPEESLRSAIWMELREIQKKRGGRITADE
                     RYEIEEELLQRLVPRFESRVVDTARFIKEHITEWWMNLH"
     gene            complement(1240..1707)
                     /locus_tag="PKZ53_18190"
     CDS             complement(1240..1707)
                     /locus_tag="PKZ53_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ42425.1"
                     /translation="MLSSAGHPRWIPISGNRAKRIIHGVINIKTGEMELLITSDWVQE
                     THQAFLHLIDVHWRGWNIIVFQDRGSPHTAEESLRIAAALQIEVRFLPRATPELNAMD
                     HLWRWVKSRGIANEPTRSVDDSADQACRYLLNLSPQERLRKAGILSGNFWLTR"
BASE COUNT          546 a          482 c          459 g          485 t
ORIGIN      
        1 gcgggacgtt gttccaatgg gcttgtttcc aggcacgact aaaattctct atgatgtact
       61 ttttcagaga acgcgaggag ccaaagagcc cacaaggttt atgcagacaa cttcggcggc
      121 acttgtcaag tgggcacgaa cttcagaaaa aactctttgg cggcatttgt cttatcttga
      181 gcgagtaggt ttggtgagac gccgaaccga ccttattcaa gacattaagt tcaagaagaa
      241 tggaggaaat atttatgaaa tatttgttcc ggaagaaatt gatttacctt tcgcacttac
      301 cattgctgaa gagagtcggc tgacaactca atccattcac agttttccac aagggaacag
      361 aagggaaggg aacagtacgg tacggacaaa agttcccgga acggaaggga acaatgttcc
      421 gtacactaca aaaacaaacc ctttaaaatc aaatatttct gagcccctaa gacatgataa
      481 tactaggtac aatacagaag aggaaagcgc cgcttcgcgg ctggcccaat accttttcct
      541 gacattcgag gaatatcttg gacgtccact ggcaacagag gaaaaactag caatctttgc
      601 aacgctctca gaagaattat tggacctgat tcaccaggga ctcaacgcca acgccgaaca
      661 tggccaaaaa caaattgaaa acccagtcgc ctggtttaca gccatgattg gtcaaaggtt
      721 gacaggtcgg aatcgcaaac gaagcccgga ggaaagtttg agatcggcca tttggatgga
      781 gcttcgagaa attcaaaaaa aacgtggagg acgaataacg gcagatgagc ggtatgaaat
      841 cgaagaagaa ttgttacagc ggctggttcc ccgttttgaa tcgagagttg ttgatacagc
      901 ccgattcatc aaagaacaca taacagaatg gtggatgaat ttgcactgaa aatcagaaca
      961 ccgtttactg aacacggcta tggcaacaat tctggcttat gccgagctcg gcataaaaat
     1021 gattatcgga aaggtcactg atgggtcacc tccgaaaacc tgtttttctg caagtagttg
     1081 ataaaaagga tttttcccat gtaggagttc ccggtaaatg tgtctggtac ccgctatgag
     1141 agtttaggga cctttgttgg gaaataccca gatttgctgg ttcaacgtct gaaattctca
     1201 ataattcccc gaccaggccg aggttgttga actgaaactc tacctggtca accaaaaatt
     1261 ccctgacaaa atcccagctt ttctgagtcg ttcttgtggg ctcaagttga gcagatagcg
     1321 gcaggcttga tccgctgagt catccactga ccgcgttggc tcattggcaa ttccccgact
     1381 tttgacccag cgccatagat ggtccatcgc attcaattct ggtgtcgcac gcggcagaaa
     1441 ccgaacttca atctgtaagg cggcggcaat tctcaagctc tcttcagccg tgtgtgggct
     1501 gccacgatct tgaaacacga tgatattcca tccacgccaa tgaacatcga tcagatgcaa
     1561 gaaggcttga tgtgtttctt gaacccagtc gctggtaatg agtaactcca tctctccagt
     1621 tttgatgttg atcactccgt ggatgatccg tttggctcga ttcccactga tcggaatcca
     1681 gcgcgggtgc cccgccgatg agagcaactc gtcagcggtg gcgtctcagt aattatcacc
     1741 tcatctaaca tcaggatgac gctatggtgg tgtccgcgca gccctttttt aatcctcctt
     1801 tcgcctgacg ccacgtttcc ggcctcaggg ccagcttata ccggggtcgc ccccagctta
     1861 tgcctgacct tcccaatgcc aaccggacac ttgccactga cgcctctacc ccacactcga
     1921 cttcaagata tttttgtaac aacctcgccg tccaaaccgt tgaattgtat cc
//
LOCUS       DAOQDO010001294         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57704_length_1969_cov_3.127482, whole genome shotgun sequence.
ACCESSION   DAOQDO010001294 DAOQDO010000000
VERSION     DAOQDO010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57704_length_1969_cov_3.127482"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKZ53_18195"
     CDS             complement(<1..886)
                     /locus_tag="PKZ53_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ42426.1"
                     /translation="MGNSSKPSTLGELRQSAWNESRLRHRSVKDEMRENLIRKLETGD
                     TLFPGIVGYEDSVIPQLINAVLSRHNFILLGHRGQAKSRILRSLENLLDEEMPVIAGS
                     EVNDHPYNPISAYGRQTIAEKGDDTQIAWMPRHARYVEKLATPDVTIADILGDVDPIK
                     AAKSGYLLSDELTIHYGLLPRANRGVFAINELPDLAGKIQVGLFNIMQEGDVQIKGYP
                     IRLPLDVLIVFTANPEDYTARGKIITPLKDRIGSEIVTHYPTSVAEAIQITEQEAWIE
                     RPQTRQVHVPRFIKQIVEN"
     gene            complement(972..1514)
                     /locus_tag="PKZ53_18200"
     CDS             complement(972..1514)
                     /locus_tag="PKZ53_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ42427.1"
                     /translation="MNQKAIMEFSHRVHSDLFRDVESLSDVDLYRQLTPGTWSIAEVI
                     EHLRQVDALLLKLFRRVVTGQKPSERRLSQRLIARVGLLAGHWMIARPLFKVNAPSFV
                     APLSTPPRHELIAGLKQVLDDLAQCEQLYSPEQLTQLYIKHPGVGALNFYEMMLIDTY
                     HFQRHLLQIQALKEKLKSGA"
     gene            complement(1542..1856)
                     /locus_tag="PKZ53_18205"
     CDS             complement(1542..1856)
                     /locus_tag="PKZ53_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42428.1"
                     /translation="MSQSRIDALTKMLAGDPSNVMVRYGLANEYWKMANYEAVIEQMT
                     AYLKATDDQGAGYRMLGQAYHKLGNLAEARAAWLAGQQAAENHGHPTMADEISQMIED
                     LE"
BASE COUNT          489 a          465 c          490 g          525 t
ORIGIN      
        1 tattttcaac aatttgctta ataaaacgcg gcacatgaac ctgccgggtc tgtgggcgtt
       61 cgatccaggc ttcctgttca gtaatttgaa ttgcctccgc caccgaagtt ggatagtggg
      121 tcacaatttc tgaaccaatg cgatctttga gcggagtgat gattttccca cgagcggtgt
      181 agtcctccgg gttggctgta aagacaatca ggacatcgag cggaagtcga atcggatagc
      241 ctttaatctg aacgtctcct tcctgcatga tattgaacaa cccaacctgg atctttccag
      301 ccaggtccgg caattcatta atggcaaaaa ccccacggtt ggcacgtggc aacaggccat
      361 agtgaatcgt cagttcatca gagagtaaat aaccgctttt ggccgccttg atcgggtcca
      421 catctcccaa aatatcagca atggtgacat caggggtggc gagtttttcc acatatctgg
      481 cgtggcgtgg catccaggca atttgagtgt catccccttt ttctgcaatt gtttgccgcc
      541 cataggcact gatcgggttg tagggatgat cattgacttc agacccggca atgactggca
      601 tttcctcatc caataaattc tccagtgagc gtaaaatccg gcttttcgcc tgacctcgat
      661 gcccaagcag aataaaattg tggcgagata aaacggcatt gatcagttgg gggatgaccg
      721 aatcttcata gcccacaatt cccggaaaga gggtgtcacc ggtttcaagt ttccggatca
      781 aattttcacg catttcatcc ttgacggaac gatggcgtaa ccggctttca ttccaggcac
      841 tctgcctgag ttctccaagg gttgatggct tactagaatt tcccataaaa cagtttcgct
      901 caggtgggtt ggtaactgat ggctggtttt tggcgactgg ttctttgttc tttggaactg
      961 agcaccagac attaagcgcc tgatttgagt ttttctttta atgcttgaat ctgaagcaaa
     1021 tggcgctgga aatgataggt atcaatcagc atcatttcat aaaagttaag cgcaccaacc
     1081 cccggatgtt tgatatagag ttgggttaat tgttcgggag aatagagttg ttcgcattgg
     1141 gcaaggtcat cgaggacctg cttcaaaccg gcaatcagtt cgtgtcgagg gggagtggaa
     1201 agcggtgcaa caaaagaggg ggcgttaacc ttaaacagtg gccgggcaat catccagtgg
     1261 ccagccagga ggccaaccct ggcaatcaga cgctgactca agcggcgttc actgggtttt
     1321 tgcccggtga caacgcggcg aaagagtttg agcaataacg catcaacctg ccggaggtgc
     1381 tcgataactt cagcaattga ccaggtgcca ggggtaagct gacggtacaa atcaacatca
     1441 gaaagcgatt cgacatcacg aaacagatct gagtgaacgc gatgactaaa ttccataatc
     1501 gctttttggt tcactcgcac ctccgtttga caaacgattg gtcactcaag atcttcaatc
     1561 atctgcgaaa tctcatctgc catcgtcggg tgaccgtgat tttcagccgc ctgttgacca
     1621 gccagccagg cagcacgggc ttcagccaga tttccaagtt tgtggtatgc ctgaccaagc
     1681 atgcgataac cggccccttg atcatcagtg gctttcaaat aggcagtcat ttgctcaatg
     1741 accgcttcgt aattggccat tttccagtat tcattggcca gcccatagcg caccatcaca
     1801 ttggaagggt cgccagccag catctttgtc agggcatcaa ttcgcgactg agacatagga
     1861 aaccttttga atgaagaatg aagaattgag aatgcagaaa ccatttagtg gttagttcct
     1921 ggttcttggt tcttggttct tggttcttgg ttcttggttc ttggttctt
//
LOCUS       DAOQDO010001295         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57750_length_1968_cov_2.993727, whole genome shotgun sequence.
ACCESSION   DAOQDO010001295 DAOQDO010000000
VERSION     DAOQDO010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57750_length_1968_cov_2.993727"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PKZ53_18210"
     CDS             complement(<1..570)
                     /locus_tag="PKZ53_18210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ42429.1"
                     /translation="MTFRDLFVDTIHTLWANKLRTTLTMFGIAWGIVSITLMVAAGEG
                     LRVGQQKVSANFGKDLMIIFAGTTSMQAGGTRAGRRIRWQDTDHIAVAQQSPDCDCVL
                     PEFGRGGSSVRSSYNSGTFLVTGSLPKFAEIRSIKVAEGRYCNEEDERMNRRVAVIGS
                     EVKKQLFGAHQAMDQDIVIRGVTYRVIGVT"
     gene            complement(607..1818)
                     /locus_tag="PKZ53_18215"
     CDS             complement(607..1818)
                     /locus_tag="PKZ53_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ42430.1"
                     /translation="MSVSVKKKFKLFRWALLLVIFGGLVAAGFGISASLKPKNDIDPS
                     KLDSVKKGDLARSVVATGKIQPLTKVEVKSKASGIVKEIKTDYGQMVKKGDILVELDK
                     EDLQARLREAKANLQAAQAALLASKATLGRNQVDAEGTDIPFLKKSMERAQQLYTQGV
                     MSKSIVEEAERAYQLAVNKQMSALQNVSVSKAEVSRAEAQVAQAQALMERSEEDLRNS
                     TIVSPMDGIVLSKNVEVGDAVSSILVLGSQATLVATLGDISSVYVLGKVDEADVGKIY
                     LKQMARIVVESFKDKQFTGEVTKISPLGIEKDNVTTFEVRVSIQNPSLELKANMSANA
                     EIILEERKNVLLIPESAVVYDKDRNTFAETPDKSVPEGKRKIAIKIGISNGVKTEVLS
                     GLSDGQSVILQ"
BASE COUNT          470 a          503 c          456 g          539 t
ORIGIN      
        1 ggtgacccca atgacccgat aagtaactcc acgaatgaca atgtcctggt ccatggcttg
       61 atgggcacca aataattgtt ttttgacttc gctgccaatc accgcgaccc gtcggttcat
      121 tcgctcgtct tcttcgttac aataccgacc ttcggccact ttaatactgc gaatctcagc
      181 aaactttgga agtgagccag tcaccagaaa cgtcccgcta ttgtatgaac tccggactga
      241 tgatccgcct cggccaaatt ccgggagcac acaatcacaa tccggcgatt gttgagccac
      301 ggcaatgtga tccgtatctt gccagcgaat gcggcgaccg gcacgggtgc cgcctgcctg
      361 catgctggtt gttccggcaa aaataatcat caagtctttt ccaaaattag cagagacttt
      421 ctgctgacct acgcgcagtc cctctcccgc agccaccatc agcgtaattg aaacaatccc
      481 ccaggcaatg ccaaacatgg tgagtgttgt tcgtaattta ttggcccaca aggtatgaat
      541 cgtatcaaca aacaggtccc gaaacgtcat ataaaggaac tttctttgaa ggaagaccga
      601 gcacaattat tggagaatga ccgattgacc atcactgagg ccactgagta cctcggtttt
      661 gacaccattg gagattccga ttttaattgc aatctttcgt tttccttctg gaacagattt
      721 gtcgggtgtt tcagcaaagg tgttgcgatc tttgtcatac accacggctg attctgggat
      781 gagcagcacg tttttgcgtt cctcaaggat aatttcggca ttggcactca tattggcctt
      841 gagttcaagc gagggatttt gaatcgaaac ccggacttca aacgtggtta cgttgtcttt
      901 ttcgatacca agcggcgaaa tcttggtgac ttcaccggtg aattgtttgt ctttaaatga
      961 ttcgaccaca attcgagcca tttgtttcaa atagattttg ccgacatcag cttcatccac
     1021 tttgcccagg acatacacgc tactgatatc gccgagtgtt gccacgagtg ttgcctgtga
     1081 cccgagcacc agaattgaac tgacggcgtc gcccacttcg acattttttg aaaggacaat
     1141 gccgtccata gggctgacaa tggttgaatt tcggaggtct tcttcgcttc gttccatcag
     1201 cgcctgagcc tgcgcaactt gagcctcggc ccggctgact tcagctttcg aaaccgaaac
     1261 gttttggagg gctgacatct gtttattgac ggcaagctgg taggctcgtt ctgcctcttc
     1321 aacaatggat ttggacatca cgccttgagt atagagctgc tgggcacgct ccatgctttt
     1381 cttcaaaaac ggaatgtcag ttccttcagc atcaacctga ttccgtccaa gcgtggcttt
     1441 cgacgccagt aacgctgcct gagcggcctg taagttggct ttggcttcac ggagccgggc
     1501 ctgcaaatcc tctttgtcga gttcaaccag aatatcgcct tttttcacca tctggccata
     1561 atcggttttg atttctttga caataccgct ggctttggat ttgacttcga ctttggtcag
     1621 gggctggatt ttaccggttg ccacaactga ccgtgccagg tcgccttttt tgaccgaatc
     1681 cagtttagac gggtcaatat catttttggg tttgagcgag gccgagattc caaatccggc
     1741 agccaccagg ccgccaaaaa tcacgagcag taacgcccac cgaaacaatt tgaacttctt
     1801 tttcacagaa acagacacag ccgccacctc tttcgaagac aaatggaacc agcccgtgga
     1861 ttcccatgtt tagggcccgg acagagttcg atgagtgtga agattaaaaa aattatgagc
     1921 gataaagatt tgattttgtg ttggttgagt ccgcttatac gtcattgg
//
LOCUS       DAOQDO010001296         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57756_length_1968_cov_2.741244, whole genome shotgun sequence.
ACCESSION   DAOQDO010001296 DAOQDO010000000
VERSION     DAOQDO010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57756_length_1968_cov_2.741244"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PKZ53_18220"
     CDS             <1..147
                     /locus_tag="PKZ53_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNJ42431.1"
                     /translation="ITNFHLPNSSLLALVCAFGGYETVMNAYRHAVEDKYRFFSYGDC
                     MLLV"
     gene            310..1830
                     /locus_tag="PKZ53_18225"
     CDS             310..1830
                     /locus_tag="PKZ53_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ42432.1"
                     /translation="MSLTSFLLQRAQHLGSKPALIDASSGRTLTYAQIADAIPRVAAG
                     LARRGFQPKDVVAFCCPNLPEFAIAFHAVAMLGGITTTINPLYTVHEMNWQLNDSRAK
                     CVITVAPLLEKIREAAQGTEVTEIFVFGEAPGATPFAELIQGPSTPPAVHINPAEDVV
                     ALLYSSGTSGLPKAVMLTHRNLIANMISQGIAAHAFTEEDTAISVLPFFHVAGLAAVL
                     EQALFKGMTLVVLPRFDLEHFLQAIQTHRVTFAPLVPPAVLALAKHPVVDRYDLSSLK
                     WIVAGAAPLGEDVARACAARLNCPISQGYGMTEASGLTHFSMDAMERGRLASVGTALP
                     GLEGQIVDLETGEPVGPGQTGELWLRGPQVMKGYLNQPDAVDEQGWYRTGDIGHTTED
                     GFVFIMDRVKELIKYKAFQVAPAELEAILNSHPAVAEAAVIPSPDLEAGEVPKAFVVQ
                     RSPVSPEELLAFVAERVAPHKKVRQLEFIDQIPKNAGGKILRRLLVERERGTKTSG"
BASE COUNT          429 a          529 c          531 g          479 t
ORIGIN      
        1 attactaatt ttcatctgcc caattcatca ctgctggccc tggtgtgtgc cttcggtggg
       61 tacgaaacgg tgatgaatgc ctatcgccac gcggttgagg ataagtaccg cttctttagt
      121 tatggcgact gtatgttgct ggtttagggt tccgggttcc gggtttttcg aaacgggtgc
      181 ggttcaaagc aacagcaccc ttactctctg tcaccctgtc acgctgccac tctgtcacct
      241 tgtcatcctg ttaccttcgg ggggttcatg atcattcgca gtcctttttc tgattttccg
      301 attccagaaa tgtcgctcac ttcttttttg ctccagcggg cacaacacct gggaagtaaa
      361 ccagccttga ttgatgcgtc cagtggacga acattaacct atgctcagat tgctgatgca
      421 ataccgcgtg ttgccgctgg actcgcacgg cgcgggtttc agcccaaaga tgtggttgcg
      481 ttttgttgtc ccaatctgcc tgagtttgcg attgcctttc atgccgtggc gatgctgggt
      541 ggtatcacga caaccatcaa tccgctctac acagttcacg aaatgaactg gcaactcaat
      601 gattcccgag ccaaatgtgt gattaccgtg gcaccgttgc tggaaaaaat ccgggaagcg
      661 gcgcaaggca ccgaagttac tgaaatcttt gtttttggtg aagctccggg ggcaacgccg
      721 tttgctgaac tgattcaagg tcccagcaca ccgcctgctg ttcatatcaa tccggcggaa
      781 gatgtcgtcg cgttgcttta ttcaagcggg acgtcggggc tgcccaaagc cgtaatgctc
      841 acgcatcgta acctcattgc gaacatgatt tcacaaggta ttgcagcaca tgcctttacc
      901 gaagaagaca ccgcgattag cgtgcttccg tttttccatg tcgccgggtt ggcggcagtg
      961 ctggaacagg cattatttaa aggaatgacc ctggtggtac ttccgcgatt tgaccttgaa
     1021 cactttcttc aggcaatcca gacacaccgg gtgacgtttg ccccgttggt accaccagcg
     1081 gttctggcac ttgccaagca tcccgtggtg gatcgctatg atctttcgag cctgaaatgg
     1141 attgtggccg gcgccgcgcc gcttggcgaa gacgttgccc gtgcctgcgc agcccgactg
     1201 aactgtccga tttcacaggg ctatggcatg actgaagcca gcggattgac ccatttcagt
     1261 atggatgcca tggaacgagg gcgactggcg tccgtgggaa cagccctgcc tggactcgaa
     1321 ggccagatcg tagaccttga aaccggcgag ccagtcggtc caggacaaac aggtgaactg
     1381 tggctgcggg ggccgcaggt aatgaaaggc tatctcaatc aacctgatgc cgttgatgaa
     1441 cagggctggt atcggacagg agacattggg cataccaccg aagatgggtt tgtgttcatt
     1501 atggatcggg tcaaggaact cattaaatat aaagcgtttc aagtggcacc ggctgaactt
     1561 gaagccattc tgaattccca tccagccgtg gctgaagccg ccgtcatccc aagcccagac
     1621 ctggaagccg gcgaagtccc caaagcattt gtcgtccaac gatctccggt ttcccctgaa
     1681 gaactcctgg ccttcgtcgc ggaacgcgtc gctccacata aaaaagttcg tcagcttgaa
     1741 ttcattgatc agattccgaa aaacgcgggt ggcaaaatcc ttcgacggct tctggtcgaa
     1801 cgcgagcgcg gaacgaaaac ctcgggctga aaaaaccagg gctgggggct gaaaaaacca
     1861 gggctggggg ttgaagaaaa cgggctcagg gcttggggat gattgaaccc gttttcttca
     1921 gcccgccttc ttcagcccgc cttcttcagc ccgccttctt cagcccgc
//
LOCUS       DAOQDO010001297         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57785_length_1967_cov_4.052824, whole genome shotgun sequence.
ACCESSION   DAOQDO010001297 DAOQDO010000000
VERSION     DAOQDO010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57785_length_1967_cov_4.052824"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PKZ53_18230"
     CDS             <1..649
                     /locus_tag="PKZ53_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687991.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNJ42433.1"
                     /translation="SIDSPARLPDPSKIEHIEEPIIRAVILTHDDFLGPILKLLEEKR
                     GTQRGFEYVGPQRVMLTYDLPLLEVVLDFFDRLKSVSRGYASLDYHLNGYQTSDLVKL
                     DVMVAGEPLDALSLIVHRDNAYQRGKLLVDKMKELIPRQLFEVPIQAAIGNRVISRQT
                     VKAAGKNVLAKCYGGDITRKRKLLEKQKEGKKRMKRVGRVEIPQEAFLAVLRVSE"
     gene            811..1521
                     /locus_tag="PKZ53_18235"
     CDS             811..1521
                     /locus_tag="PKZ53_18235"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNJ42434.1"
                     /translation="MSIPPIPSLEGSRTLPLFPLPLVLFPGAMLPLHIFEPRYKEMIK
                     DCLDGDRLFGITFLDSKEGWPPPTGRIGCAAFILATVPLQEGRMNLLTTGVMRYRTLE
                     YIETRSYLQAQVEFFQDAATDLNLAELADQVTTLYRRTIRATQVIQNNDLTEDELPDD
                     PEELSFSIASTLQLSTAHRLELLELTSVQARLERLKKMLQGVVGQYELRATVHEKAKT
                     NGHGSHKTLAQLLEEERT"
     gene            1666..>1967
                     /locus_tag="PKZ53_18240"
     CDS             1666..>1967
                     /locus_tag="PKZ53_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNJ42435.1"
                     /translation="MNTIGLTVEALQNALAARETTATEVCTAVLDRIEALNPELNAFI
                     TITRDRALAQAAQVDADLAAGKPFQALTGVPIAVKDNQCLKGVRTTCASKILGNFQP"
BASE COUNT          483 a          543 c          489 g          452 t
ORIGIN      
        1 cagtattgac agcccggccc ggctcccaga cccgtcaaaa atcgaacaca tcgaagaacc
       61 catcattcgg gcggtgattt tgacccacga tgacttcctt gggccgattc tgaagctatt
      121 ggaagaaaaa cgcggcaccc agcgtggatt tgaatatgtt ggccctcagc gcgtgatgtt
      181 gacctatgac ctgcccttgc tcgaagtggt acttgatttc tttgaccggt tgaaatcagt
      241 ctcacgcggc tatgcctcgc ttgattacca tttaaacgga taccaaacct cggacctggt
      301 caaactggat gtgatggtgg ctggcgaacc actcgacgcc ctgtcactca ttgtccaccg
      361 tgataatgcc tatcaacgcg gtaaattgct ggttgataaa atgaaggaac tcattccacg
      421 tcagttgttt gaagtcccaa tccaggcggc catcggaaac cgcgtcattt cacgccagac
      481 cgtaaaagct gctggcaaaa acgtgctggc caagtgttat ggcggcgata tcacgcgaaa
      541 acggaagctg cttgaaaagc aaaaagaagg caaaaaacgg atgaagcgcg ttggccgggt
      601 tgaaattcca caagaagcat ttttggctgt cctgcgcgtg agtgagtaat ccatctgagg
      661 gttcggggac gagtgagtac cacccgcgtg agtgggtggg gtgagaatcc aaccagccca
      721 cccactcacg cgggtggtac tgactatcgg ttctttgtgt atcctgattg ctgaacccca
      781 aaccccatcc cctgaaccca caatgtctct atgtcaattc ctccgattcc gagtttagaa
      841 ggttcacgaa cgctgccact gtttccctta ccgctggtgc tgtttccagg agcaatgctg
      901 ccgctccata tttttgagcc gcgatataag gaaatgatca aggactgtct ggacggagac
      961 cggctgtttg gcattacctt tttggatagc aaggaaggct ggcccccgcc aactggaaga
     1021 attggctgtg cagcctttat cctggcaacg gttccactcc aggaaggccg catgaacctt
     1081 ctgaccaccg gcgtgatgcg ctatcgaacc cttgaatata tcgagaccag atcttatctt
     1141 caggcacagg ttgaattctt tcaggatgca gccaccgacc tcaaccttgc cgaactggcc
     1201 gatcaagtca caaccctgta ccgccggacc atccgggcca cccaggtcat ccaaaacaac
     1261 gatttgacgg aagacgaatt acctgatgat ccagaggagt tgtcgttttc gattgccagc
     1321 acgcttcaac tcagcaccgc ccaccggctt gaactcctgg aactgacatc ggttcaggca
     1381 cggttggagc gcttgaaaaa aatgctccag ggtgttgtcg gacaatatga actgcgggcc
     1441 acggtccatg aaaaagccaa aaccaatggc catggctcac acaaaacgct cgcccagttg
     1501 ctggaagaag aacgaacctg agaaaattgc gattgacccg atcaggttgt ttgccttgcc
     1561 ccagagcttg ataacacaac cctcatcctg atcacgtggt tctgttttct tcattcttca
     1621 ttcttcattc ttcattcttc attcatttag gggaggaatc tgaacttgaa caccatcggt
     1681 ttaaccgtcg aggcgcttca gaacgcactc gccgcacgcg aaacaaccgc cactgaagtc
     1741 tgcacagccg tgctggaccg cattgaggct ttaaacccgg aactgaatgc ttttatcacc
     1801 atcacccgtg atcgggccct ggcccaggcg gctcaggtgg atgcggatct ggccgctgga
     1861 aaacccttcc aggcgctgac gggtgttccg attgcggtca aagacaatca gtgcctcaag
     1921 ggtgtccgaa ccacctgcgc ttcgaaaatt ctgggaaatt tccagcc
//
LOCUS       DAOQDO010001298         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57810_length_1967_cov_2.638075, whole genome shotgun sequence.
ACCESSION   DAOQDO010001298 DAOQDO010000000
VERSION     DAOQDO010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57810_length_1967_cov_2.638075"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1203)
                     /locus_tag="PKZ53_18245"
     CDS             complement(88..1203)
                     /locus_tag="PKZ53_18245"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNJ42436.1"
                     /translation="MTNLSPSTVTSYTQADMDDPFSGYSTTSQTMLAHVLVEPGRLEM
                     REVTRPVPSPGGVVIRVRAALTCGTDLKAFLRGHPKFPMPTLFGHEFSGVIAEVGAGV
                     TQFREGDAVMSTHSAPCGTCYYCQRNQENLCDTIMGTMVLGAYAEYIHIPEHIVRQNM
                     YHKPIELSYTEAALLEPLSCVLHGLETIRFREDDTVLIIGSGAIGLLHLLALKAYGVQ
                     NILVAGRRSFRLKMAKSMGAAEVIDVRHDDIQESVIELTGGRGADTVIECTGRPEVWE
                     SAVSMARRGGNVILFGGCKKGTSVTFDTHRLHYDQITLHSPFHMTPASVRKAFQLLTE
                     KGIDGHNLITGEYRLDQLLEVFDLLQNSDCIKCAVIP"
BASE COUNT          510 a          492 c          484 g          481 t
ORIGIN      
        1 attcgccatt ggtttttccg catcacctct taaacatcaa ctacggctcg atattggtgg
       61 cagtgtggcc caccactggc acctggctta aggaatcacg gcacatttga tgcaatcgct
      121 gttttgaagc aaatcgaaaa cttcgagcaa ctgatcgagc cggtattcac cggtaatcag
      181 attgtgtccg tcaattccct tttcggtcag caattggaag gctttgcgaa cagaagccgg
      241 tgtcatatga aatggactgt ggagcgtgat ttgatcatag tgcaaccgat gtgtgtcaaa
      301 ggtgacactg gtgccttttt tgcagccacc aaaaagaatc acatttccac cacgacgggc
      361 catcgaaacg gctgattccc agacttcagg ccgtccggta cattcaatca ccgtatcggc
      421 accacgccca cccgttaatt cgatcaccga ttcctggata tcatcgtgtc ggacatcaat
      481 gacttcggca gcgcccattg atttggccat tttcagtcgg aaagaccgtc ggccagccac
      541 gagaatgttt tgaacgccat aggctttgag tgccagtaaa tgcagcaagc cgatagcccc
      601 gctcccaatg attaaaaccg tgtcgtcttc gcgaaagcgg atggtttcaa gcccatgcag
      661 aacacaggaa agcggctcaa gcagagccgc ttcggtataa ctgagttcaa ttggcttgtg
      721 atacatgttt tgtcgcacga tgtgttcggg aatatggata tattccgcat aggcacccaa
      781 caccatggtg cccatgatcg tgtcacatag attttcctga ttgcgctggc agtagtaaca
      841 ggtgccacaa ggcgctgagt gcgttgacat caccgcatcg ccttcgcgaa attgagtcac
      901 cccggcgcca acttcggcga tgacaccaga aaattcgtgg ccaaacaggg ttggcattgg
      961 aaattttggg tgtccacgaa gaaaggcttt taaatccgtg ccacaggtta atgcggcccg
     1021 aacccgaatc accaccccac ctggactggg gacaggtcgg gtcacctcgc gcatttccag
     1081 gcgtcctggc tcgaccagaa cgtgggctaa cattgtttgc gaagtggtcg agtagccaga
     1141 aaatggatca tccatgtcag cttgagtgta agaagtaaca gtacttggag aaagattagt
     1201 cattgtagat agagccaagt ttctaggaaa ggctaaaaag caggctgcat tttcctcatg
     1261 atgatgaggt gtggcaagtc aactttgaaa aaacctcttc agccgggtgc gaaaaggttg
     1321 agcccttgct gtaaaattgt taccaactgc aatcagatca atcactttga tggggagttc
     1381 agattgtaga gtgctttgca tcacaaagtc aacctaaaag acaggttggc tggcgcaccg
     1441 cgactaaatt caaaaagcgg gggcggcttc cccctctcac ttttgaccgg aaaggtggcg
     1501 gtgagccttc aaatccaggg tttggggttc aattcaccca caaagaaaca ggttaaaacc
     1561 ctttgatagg ccgtacagac cgcccaacac taaaaaggtt ctatcttcag aaccattttc
     1621 cttttggcat tgatcttgag aacctaagtg gttgcttgac aatcccaata tcaagtcgct
     1681 aatgtttgcg aactcgcagc acactatctc ccccacaatc aaatctgctt catcgctgca
     1741 acagcttcgg atacctgggg tctgtgtgcc aggcaaagag gcggtgtttt gccgttgttc
     1801 acattccaca ttcgcaaagg aaccacacta tgaaagttgg tattttggtt ggccgagaaa
     1861 atacgtttcc gcctgcgttg attgaacgga ttaactccag cggcaccgga gtaactgccg
     1921 aatacgtcaa tatcgggggt gttcgcatgg atggcccctg cccctat
//
LOCUS       DAOQDO010001299         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57860_length_1966_cov_2.277865, whole genome shotgun sequence.
ACCESSION   DAOQDO010001299 DAOQDO010000000
VERSION     DAOQDO010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57860_length_1966_cov_2.277865"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKZ53_18250"
     CDS             complement(<1..1130)
                     /locus_tag="PKZ53_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42437.1"
                     /translation="MNSTTESTQTCRGSILIVDDNPVNLKLLSGMLVKENFHVKAAIN
                     GRRALAQTQFMKPELIMLDISMPDIDGYEVCRQLKANEATRDIPIIFISALDEVIDKV
                     KAFQVGGVDYVTKPFQFEEVLVRIETQLQMSRLQKELISKNEALLRKNEELIQANRRA
                     NLIFSAMSDMLPGSILDGKYRLEKKIGEGGFGAVYQAIHLGLNRSVAIKIFQPSRRTD
                     MMKEQNRFRLEGISACRVNHPNAISILDSGLTSEGVTYLVMELLEGNTLAQELRQKKT
                     LSTMRCAEVLLPVCEVLDVAHKAGIIHRDIKPENIFLHQTQNGEVIKLVDFGIAKLFG
                     GEASDEFDSLTMPGTVIGTPAYMSPERLSNQSYDGKADVYSLG"
     gene            complement(1127..>1966)
                     /locus_tag="PKZ53_18255"
     CDS             complement(1127..>1966)
                     /locus_tag="PKZ53_18255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42438.1"
                     /translation="NPTLTESKVKTGPLGQPRVLVVEDNKVNQILAKITLENLNVRVD
                     LAEDGFKALEALAKISYDLIFMDCQLPLMDGFETTAVIREQEMLSNRHIPVIALTANA
                     MRGDRERCLAAGMDDYVSKPFQPQDFQSILERWLPKVSETPTPESEPPILPLEQSYQP
                     DEKVLSSDSLEQLMTLADGDGSFLHDFLELAYTNFAEHITTLHKTVPLTKRKRLRETA
                     HSLKGACATIGAATMVDLCHQLEEFAAETPVSTVQDLITQLEQAFTQVKTEMTAYLRN
                     KRL"
BASE COUNT          452 a          481 c          496 g          537 t
ORIGIN      
        1 cctaaactat acacatcagc tttgccgtca taggactgat tactcagccg ttcgggggac
       61 atataggctg gtgttccgat cactgttccg ggcatggtta agctatcaaa ctcatcactg
      121 gcctcccccc caaataattt ggcaattcca aagtcaacca gtttgatgac ctcaccattt
      181 tgtgtctggt gaagaaaaat attctcgggt ttgatatcgc gatggatgat accggctttg
      241 tgagccacat cgagcacttc acagaccgga agtaacactt cggcgcaacg catggtcgag
      301 agagttttct tttgtcgtaa ttcctgagcc agggtattgc cttccaggag ttccatcacc
      361 agataggtca cgccttcaga ggttaacccg gaatccagaa tcgaaattgc atttggatga
      421 ttgacccgac aggcggaaat tccttccagc cggaagcggt tttgttcctt catcatatcg
      481 gtgcggcggg aaggctggaa aatttttatt gccaccgacc ggttgagccc caggtggatc
      541 gcttgataga cggccccaaa cccaccctca ccgattttct tctccagccg gtatttccca
      601 tccaggattg acccaggcag catgtccgac atggctgaaa aaatcaggtt tgcccggcga
      661 ttggcctgaa tcagctcttc gttttttcgc aagagggctt catttttgct gatgagttcc
      721 ttttgtaacc gggacatttg taactgggtt tcgatgcgaa ccaggacctc ttcaaactgg
      781 aaaggtttgg tcacataatc aacgcctccg acctgaaagg ctttgacctt atcaatcact
      841 tcgtcaaggg cgctaataaa gatgatcgga atatcacgtg tcgcctcgtt ggctttgagc
      901 tggcggcaga cctcataccc atcaatatcc ggcatgctga tatcgagcat gatgagttcc
      961 ggtttcataa actgggtttg ggccagtgca cgccggccat taatcgcggc tttgacgtga
     1021 aagttttctt tgaccagcat gcctgaaagc agctttaaat tcactggatt atcatcaaca
     1081 atgagaatac ttccgcgaca ggtttgagtg gattcagtgg ttgaattcat aggcgtttgt
     1141 tcctgagata agccgtcatt tcagttttta cctgggtgaa ggcctgctct aactgagtga
     1201 tcaggtcctg aaccgtcgaa accggggttt ccgctgcgaa ttcttccaac tgatggcata
     1261 gatccaccat ggtggcggcg ccaattgtgg cgcaggcacc cttgagggaa tgtgcggttt
     1321 cacggagtct tttgcgtttg gtgagtggca ccgttttatg caaggttgtg atgtgctctg
     1381 caaaattggt gtaagccagc tccagaaaat catgtaaaaa cgagccgtcc ccgtcagcca
     1441 gcgtcatcag ttgttccagc gagtctgaac tcagaacttt ttcatctggc tgatatgatt
     1501 gctccagggg gaggatgggc ggctcagatt ctggcgtcgg cgtttcagat actttgggta
     1561 accagcgttc aagaatagac tggaaatctt gtggttggaa aggtttactg acatagtcat
     1621 ccattcctgc cgccagacat cgttcacggt ctccgcgcat tgcattggcg gtgagggcaa
     1681 tgactggtat atgccggttc gacaacatct cttgttctcg aatcaccgcc gtggtttcaa
     1741 agccatccat gagcggaagc tgacaatcca taaagatcag gtcatagctg atcttggcca
     1801 gcgcttccag ggctttgaag ccatcttcgg ccagatcaac ccgaacgttg aggttttcaa
     1861 gcgtaatctt ggccaggatc tgattcactt tattatcttc gaccaccagc acgcgaggtt
     1921 gacccagtgg gccagttttt accttggatt ctgtcagggt tgggtt
//
LOCUS       DAOQDO010001300         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57868_length_1966_cov_1.969649, whole genome shotgun sequence.
ACCESSION   DAOQDO010001300 DAOQDO010000000
VERSION     DAOQDO010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57868_length_1966_cov_1.969649"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="PKZ53_18260"
     CDS             complement(<1..1653)
                     /locus_tag="PKZ53_18260"
                     /inference="COORDINATES: protein motif:HMM:NF020701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNJ42439.1"
                     /translation="MKLKVTWMSLCAVIAQSLFVFPAFAATQAAKGQPGVTSSWTTGN
                     KTGLGTSLSVRSKVWFTLADGQLTEVYYPRLDVANLRNLDFIITDTKGYRRLESLHAV
                     RRETTWTDKNSLSFTQITSDGARWELTKTYTTDPNRNTLLVQVNFRPLEKNDKRPYQI
                     YALIDPSISNSGLKDTGYTEAKALVASEGNIAMATVARPAFSMTSSGFAGVSDGWTDL
                     TEDGRLDALETRAANGNVVQIGQLAGYDSTLAFGFGESAQSALDEARGSLKDGFAAVK
                     KAYADEWASLVKKLPATSPEFRDTFTMASMTLYALEDKTFRGASIASLSIPWGEVANA
                     DDPNIGGYHLVWSRDLYQVATAFLALGDKEAANRALDYLFEKQQRPDGSFPQNSWLDG
                     RPFWTSIQMDEAAFPLILAWQLERFDENTYRQHIRPSAEFIVKTGPETQQERWEEESG
                     YSPSTIAAEIAGLICAADIARKLKKNDDADRYVKKANEWATGVETWCYTTTGPHDAGE
                     RERGYYFRINDDKDPNDGAKIEINNGGGTYDERSIIDGGFLEL"
     gene            complement(1725..>1966)
                     /locus_tag="PKZ53_18265"
     CDS             complement(1725..>1966)
                     /locus_tag="PKZ53_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HNJ42440.1"
                     /translation="KARRLIEALGGVSNIQRVDACAETRLRLVLSNPAKIDESALKAA
                     GSGGVMKLAGGVIHLLVGLNSDQYAAEINAQLAHQ"
BASE COUNT          422 a          511 c          535 g          498 t
ORIGIN      
        1 aagttctaaa aagcctccat caatgatcga acgctcgtca taggtgccgc cgccattgtt
       61 gatttcgatt ttggcgccat cattggggtc tttgtcatca ttgattcgga aataatagcc
      121 gcgttcgcgt tcaccggcat cgtgtggccc ggtcgtggtg taacaccagg tttcaacgcc
      181 agttgcccat tcgttggctt ttttcacgta gcggtccgca tcgtcatttt tcttgagttt
      241 gcgggcaata tcagcggcac agatcaatcc agcaatttca gcagcaattg tggatggcga
      301 atagccgctt tcttcttccc agcgctcctg ctgggtttca gggccggttt ttacaataaa
      361 ttcggctgat ggccggatgt gctgacgata ggtgttttcg tcaaagcgtt cgagttgcca
      421 cgccaaaata agtggaaagg cggcttcgtc catctgaatc gaagtccaaa atggtcgccc
      481 atccagccag gaattttgtg gaaagctgcc atctggtcgt tgttgtttct cgaacaagta
      541 gtcaagtgcc cggttggcgg cttctttgtc acccagtgcc agaaacgcgg ttgcgacttg
      601 atagaggtcg cgcgaccaga cgagatgata ccccccaatg ttgggatcgt cggcgttggc
      661 gacttcgccc cagggaatgc tcaaactggc aattgacgcg ccccgaaagg ttttatcctc
      721 cagggcatag agggtcattg aggccatcgt aaacgtgtcg cgaaattctg gggaagtcgc
      781 cggcagtttt ttgacaagtg atgcccattc atcagcatag gcttttttca ctgccgcaaa
      841 gccgtctttt aacgagccac gggcttcatc gagagccgac tgagcagatt caccaaaccc
      901 aaaggcaagg gttgaatcat acccggcaag ctggccaatc tgaacgacat ttccgttggc
      961 cgcccgagtt tcaagcgcat ccagcctgcc gtcttcggtg agatcggtcc agccatccga
     1021 aacgccggca aatccgctgg aggtcattga aaacgctggt cgcgccaccg tggccatggc
     1081 gatgttgcct tcgctggcaa ccagggcttt ggcctcagta tatccggtgt ctttcaggcc
     1141 gctatttgaa attgatgggt caatcagggc atagatctgg tatggccgtt tgtcattctt
     1201 ttcaagcggg cgaaagttga cctggacgag caaggtgttg cgatttgggt cagtggtata
     1261 ggttttggtc agttcccagc gggcgccatc actggtgatc tgggtaaaac tcagggagtt
     1321 cttatcggtc caggtggttt cgcgtcggac ggcgtgcagt gattcaagtc ggcgatagcc
     1381 tttggtgtcg gtaataatga aatcgagatt gcgcaggttg gccacgtcca gccgggggta
     1441 gtagacctca gtcaactgac catcggcgag ggtaaaccag acttttgatc gaacgctcag
     1501 gctggtgcca agcccggttt tgtttcctgt ggtccaggaa gacgtaacac ccggctggcc
     1561 tttggctgct tgagttgctg caaaggctgg gaaaacaaac aggctttggg cgatcacggc
     1621 gcacaaactc atccaggtta ccttcagttt cacaatccat ctctcctttg agtggttgac
     1681 gtcaccccag gtcaagccgg gtaaaagtca gcctccagat ctgattattg atgggccagt
     1741 tgagcattta tttcagcggc atactggtca gagttcagac ccaccagcag gtgaatcacg
     1801 ccgccagcca gtttcatcac gccgccactt cccgccgctt tcaaagctga ttcgtcaatt
     1861 ttggccgggt ttgacagcac cagccgtaag cgggtttcag cgcaggcatc aactcgctga
     1921 atatttgaaa ccccacccag tgcctcaatc aatcgtcgag cttttt
//
LOCUS       DAOQDO010001301         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57906_length_1965_cov_2.954450, whole genome shotgun sequence.
ACCESSION   DAOQDO010001301 DAOQDO010000000
VERSION     DAOQDO010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57906_length_1965_cov_2.954450"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1289
                     /gene="tssA"
                     /locus_tag="PKZ53_18270"
     CDS             168..1289
                     /gene="tssA"
                     /locus_tag="PKZ53_18270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HNJ42441.1"
                     /translation="MPVLAPSFDFELLFKPISAESPCGEDLRFSFEYDTLMEARRSDD
                     QFADTIGGNLKMADWDEVYERGTRLLTTQTKDLQIAVWMVEAMINLHGLVGLRDGLKL
                     VEGIMSQFWGHLHPQPDDDNDFEGRANALSWMDREVSSLMTQIPFSQGVSNHIYSFAQ
                     WKEAEKYKLPDTSFGLSGEEQMKYEELKAEAEAEGKITSEQWKLGETTTSREFYQTLC
                     SILGECTGAFVELDQTIDNRFGRSAPGLNHLKKTLTEMQDLVIRILKDKGGVPGITTP
                     PLQSEPFGPATTQFSPEQRGTEPADTQYGPIRSRQDALKMLADVATYYRQAEPHSPIP
                     YLIERAIRWSNLSLQEWLREALKSDDALSNVQDLLGLGM"
     gene            1349..>1965
                     /locus_tag="PKZ53_18275"
     CDS             1349..>1965
                     /locus_tag="PKZ53_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42442.1"
                     /translation="MTHYCDRVIGWLNHLKTQTDSSSHLRIAVLGPQRRHLFEVAEQF
                     PGAVLINGEHDLWPLDLMALGKQPYPIVFLLTPSRRVAEHFFELATSQTKQPVHRVTL
                     WLGEHFRRISFPQNADENWQMLLGAPLMADSAPPVVESRSLYAPRLRTSPRTTSLRFF
                     SDLQLDPVNERQVASIERLVDLLERYDQTIAVEAPECRSDAIHLIS"
BASE COUNT          486 a          512 c          486 g          481 t
ORIGIN      
        1 caatcaagca ctgaccaaat ccaatatctc aggaaactta tgacaaggta cttataagat
       61 tttggttact ggatcctggg gattttggtt tcccagtacc agggaccagt cgccaccaca
      121 aaaatcttga tttgacttca tatttctttc cgaaggagcg tcttcttatg ccagtcctgg
      181 ccccgtcgtt tgactttgaa ttgcttttca aacccatctc agccgaatcg ccctgtggcg
      241 aagatcttcg gttttcattt gagtatgaca cgttgatgga agcccggcgc tctgatgatc
      301 aatttgctga taccatcggc gggaatctaa aaatggctga ctgggatgaa gtctatgaac
      361 gtggaacccg tctgctgacc actcagacca aagacttgca aattgcggtc tggatggttg
      421 aagccatgat taacctgcac ggtctggtgg ggttgcggga tgggttgaag ctggtcgaag
      481 gcatcatgag tcagttctgg ggtcatttgc atccgcaacc tgatgacgac aatgactttg
      541 aaggccgagc caatgcactc tcctggatgg accgcgaagt atcctcgctg atgacccaaa
      601 ttccattttc tcagggtgta tcgaatcaca tttacagctt tgcccagtgg aaagaagctg
      661 agaaatataa attgccggat acttcgtttg gactttcagg cgaagagcaa atgaaatatg
      721 aggagctgaa agctgaagct gaagccgaag gaaaaatcac cagtgagcag tggaaacttg
      781 gtgaaaccac cacctcgcgt gaattttacc agactttatg ttccatcctg ggcgaatgta
      841 caggcgcctt tgttgaactc gatcagacca ttgataatcg ctttggtcgc tcggcgccag
      901 gactcaacca cctgaaaaaa acactgactg aaatgcagga tctggtcatt cggattctca
      961 aagataaagg aggtgtcccc ggaataacca caccaccact tcaatcggaa ccgtttgggc
     1021 ccgccaccac tcaattctca cctgaacagc gaggaaccga acctgctgac acccaatatg
     1081 gtccaattcg ttcgcgtcag gatgcactca aaatgctggc tgatgttgcg acctactatc
     1141 gtcaggctga accacatagc ccaattccct atttgatcga acgcgctatc cgctggtcaa
     1201 acctgtcgct ccaggagtgg ttgcgtgaag cactcaagag tgacgacgcc ctgagcaatg
     1261 tacaggatct gttaggcctt ggaatgtaac acttttttcg gagtgcggtg gcaggacacc
     1321 gcgttgtcat ttcaccagcg gagcttttat gactcactat tgtgaccgcg tgattggctg
     1381 gctcaatcat ctgaagaccc agacagatag ttccagtcac cttcgaattg ccgtgctcgg
     1441 cccgcaacga cggcacctgt ttgaggtcgc cgagcaattt cccggagccg tgctgatcaa
     1501 tggtgaacac gacctgtggc cgctggatct gatggcgctt ggaaaacaac cgtatcccat
     1561 cgtttttcta ctcaccccaa gccgacgggt cgccgaacat ttttttgaac ttgcaaccag
     1621 tcaaaccaaa cagccagtgc atcgggtaac gttgtggctg ggtgagcact tccggcgaat
     1681 ttcattcccg cagaatgccg acgaaaactg gcaaatgttg ctgggcgcac cactgatggc
     1741 tgattcggcg cctccagtcg ttgaaagtcg ctcactgtac gcgccccggc tgcgaacttc
     1801 acctcggaca accagcctgc ggtttttttc agacctccag cttgatccag taaatgagcg
     1861 acaggttgcc agtatcgaac ggctggttga tctgctggaa cgctatgatc agaccatcgc
     1921 ggtcgaagcc cccgaatgcc gctcagacgc cattcatttg atttc
//
LOCUS       DAOQDO010001302         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_57909_length_1965_cov_2.799476, whole genome shotgun sequence.
ACCESSION   DAOQDO010001302 DAOQDO010000000
VERSION     DAOQDO010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57909_length_1965_cov_2.799476"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1134)
                     /locus_tag="PKZ53_18280"
     CDS             complement(130..1134)
                     /locus_tag="PKZ53_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNJ42443.1"
                     /translation="MKRQRPIILGIVGDSAAGKSTLTRGLAQLIGPERVTCVCTDDYH
                     KYDRRERAEHKITALHPDCNYLDVLEQHLERLHYGQPILKPVYDHSTGSLVRPEYVQP
                     SEFVIVEGLLGYHSAVMRQFYDVKVYLSPPEELRRVWKVKRDTSKRGYQPAQVLSELE
                     RREPDSQDFIRPQREFADIVVQFYPPEGISPEEAGGDLNVRLILRPTIRHPDLTYLSE
                     NKVSSEAGVRMNLGRDSGKPVDILEIDGGVLKHHAVQLQETIWKHLPEQNQLTEDQFG
                     NYIDNDDVRHSAPLAITQLLLAYHLLREYSGERQMIFAPPVSALSRVQTVSPNRRTAR
                     "
     gene            complement(1257..>1965)
                     /locus_tag="PKZ53_18285"
     CDS             complement(1257..>1965)
                     /locus_tag="PKZ53_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ42444.1"
                     /translation="TLHMSFTGRPGTGKTTVAMRMAQILHRLGYVRRGHLVVATRDDL
                     VGQYIGHTAPKTKEILKKAMGGVLFIDEAYYLYRPENERDYGQEAIEILLQVMENQRD
                     DLVVILAGYKDRMETFFQSNPGFHSRIAHHINFPDYTLAELMAIAELMVQQQMYRFDE
                     HSHRAFAEYLQFRIQQPHFANARSVRNAIDRIKLRHANRLVQQGGIISQEELLRIDEQ
                     DVRQSRVFQSGSEPQTN"
BASE COUNT          458 a          506 c          477 g          524 t
ORIGIN      
        1 ccacattggc tgcttcctgc cgctgtcttg tccgttcatc ttctttgatt actgcttgtt
       61 gaacactcgc aatatgatca gtcatggtgt tcccttcctc tctctcggaa atttcaacct
      121 cacaccgtct caccgggccg tgcgccgatt tggagaaact gtctgcaccc ggctcaaggc
      181 tgagaccggt ggtgcaaaga tcatctgtcg ttcacctgaa tattcccgca agaggtgata
      241 ggccagtaac aactgtgtga tagccagcgg tgcactgtgt cgcacatcgt cgttgtcgat
      301 atagttgcca aattgatctt cggtcaactg gttttgctca ggcagatgtt tccagatggt
      361 ttcctggagt tgaactgcgt ggtgtttcaa cacgccaccg tcaatttcca aaatatccac
      421 tggtttccca ctatcgcgtc cgaggttcat ccgaacgccg gcttcagaac tgactttgtt
      481 ttctgagaga taggttaaat cgggatggcg aatggttggc cgcaggatca gccgcacatt
      541 gagatcgccg ccagcttcct cgggggaaat tccttcaggt ggataaaact gaacaacgat
      601 gtcggcaaac tcccgttgtg ggcgaatgaa atcctgcgaa tccggctcac gtcgttcaag
      661 ttcggaaagt acctgtgctg gctgataccc gcgtttggag gtatcgcgtt tgactttcca
      721 cacccggcga agttcttccg gtgggctcaa atacactttg acatcataaa attggcgcat
      781 caccgccgag tgatagccaa gcagtccttc cacaatgaca aactcagatg gctgcacata
      841 ttcaggccga accagcgacc cggtcgaatg atcatacacc ggcttgagaa tcggctggcc
      901 atagtggagt cgctccaggt gttgttccaa cacatccaga tagttacagt caggatggag
      961 cgccgtaatt ttatgttctg cccgctcacg ccgatcatat ttatgatagt catctgtaca
     1021 gacacaggtc acccgctcgg gaccgatgag ctgtgccagg ccgcgggtga gcgtggattt
     1081 gccggcagca ctgtcaccta caatccccaa aataattggt ctttgtcttt tcataacagc
     1141 tccatgaaaa gaattaagaa ttaggaatta agaatgaaga aaagctattt cattgaataa
     1201 acagagcttg cttttctcga aattcataat tcatccctca taattcgttt gtgtggtcaa
     1261 ttcgtttgtg gctccgatcc gctttgaaat acccgacttt gacggacgtc ttgttcatca
     1321 atgcggagga gttcctcctg agaaattatc ccgccttgct ggaccagtcg gttggcatgg
     1381 cgcaatttga ttcgatcaat ggcattgcgc acactgcggg cattggcaaa gtgaggttgt
     1441 tggatccgaa attggaggta ttcagcaaaa gcccggtgtg aatgctcgtc aaaccggtac
     1501 atttgctgct gaaccatcaa ctcggcaatc gccatgagtt cagccagggt gtagtccgga
     1561 aaattgatgt ggtgcgcaat tcgggagtga aacccaggat tggattgaaa aaacgtttcc
     1621 atccggtctt tgtacccggc caaaattaca accaggtcat cccgctgatt ttccatcacc
     1681 tgaagcaaaa tctcgatggc ttcctgtccg taatcgcgct cattttcagg tcggtacagg
     1741 taataggctt cgtcaataaa cagcacgccg cccattgcct ttttcagaat ttctttggtt
     1801 ttgggcgctg tatgcccaat gtactgtcca accagatcat cgcgggttgc cacgacgagg
     1861 tgcccacggc gcacataccc caatcggtgc aaaatttgcg ccattcgcat tgccaccgtg
     1921 gtttttcctg ttcccggtcg cccagtaaaa ctcatgtgga gcgtt
//
LOCUS       DAOQDO010001303         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58022_length_1963_cov_2.950210, whole genome shotgun sequence.
ACCESSION   DAOQDO010001303 DAOQDO010000000
VERSION     DAOQDO010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58022_length_1963_cov_2.950210"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKZ53_18290"
     CDS             complement(<1..528)
                     /locus_tag="PKZ53_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42445.1"
                     /translation="MRSCFLAVVFFLSQLTQLTVPILAEVPEKTEVLPNSPYRTQTLQ
                     LDCGADLYTIFAESESGESIPMVSFVNDTFGDTDPTNDQLRYVWVYSYCSPSLAQKVN
                     AAIPFLYHRVASSKNDQPTEAPPVLFDFSKPLHSTWNRIQWLAIQLSVLDPHGWVWQA
                     SSRTYVRNRQANKQAH"
     gene            972..1805
                     /locus_tag="PKZ53_18295"
     CDS             972..1805
                     /locus_tag="PKZ53_18295"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ42446.1"
                     /translation="MHYSPINLGKTLKKADLPDFVVTEALHKPMTVLDRHAHEHACMA
                     FLYRGFYTEKVGSKLLECPQHSLLIRPSGFEHSNDYQRTGAASIIIEVKQSWLHQFEQ
                     QSTLFQDASVVQHPVLVPTFQRMYDEFHSPQPLSDLYLEGLILEVLVQSTRLQSNLPE
                     SGQPLWLKAVVELIEDDFSRDLSLAEIATVAGVHPSHLTRVFRQQFHCSIGEYVRRVR
                     ITFAKHQLAETDCSLAEIAMAAGFFDQSHFSNVFRTIAGQSPLKYRQANRKCSSTPKM
                     S"
BASE COUNT          500 a          470 c          462 g          531 t
ORIGIN      
        1 gtgagcctgt ttattggcct gacggtttcg gacataggtt cgcgaagacg cctgccagac
       61 ccacccatgc gggtccaaaa ccgaaagctg gatggccagc cattgaattc gattccaggt
      121 ggagtgcaac ggcttcgaaa aatcaaaaag aacgggcggg gcttcagtcg gctgatcatt
      181 tttcgaggag gcaacgcgat gatacaaaaa gggaatcgcg gcatttactt tttgtgccag
      241 cgatggcgag caatatgaat agacccagac atatcgaagc tggtcattgg ttgggtcggt
      301 gtcgccaaac gtgtcattga caaaagaaac catagggatg ctttcgccag actcagattc
      361 ggcaaaaatg gtgtacaggt cggcgccaca gtctaattgc agggtttgcg tccggtaagg
      421 tgagtttggc aaaacttcag ttttttcagg tacttcggcc aggattggta ctgtcagttg
      481 agtgagctga cttaaaaaaa acacaacagc cagaaaacag cttcgcattg tggatcctca
      541 ttttctctgt ctttgggttt tcataccagt ttgcaataaa acgtttgtct ttgaagacgg
      601 tcaagtaatt caatcaagtg aacttagtga actacaggct actgactaca aactggtcaa
      661 ctatttggaa cactgcggcg tgctgacgtc caacaatttt attttgggag tgttttgaat
      721 aaagccggtg atcaaaacat ccagagtgga tgagccggca tcgcctgggc gttgcctggt
      781 ttgggttgtt tttgctttct caatcctgat cagcgtactg gttctgattt gaattgaccc
      841 gtctggttgt ctcagttttg tcggctgtga cacggctgga aaaaacaaaa aatctgagat
      901 ttatttcaag ttgagccaat ttcaagtagg ctgtttgcac ctggattgtt atgttttgga
      961 tgagaaccac tatgcactac agcccaatca atcttggcaa aaccttaaaa aaagcggact
     1021 tgcctgactt tgttgtcacc gaggcactgc ataaaccaat gacggtgtta gatcgtcacg
     1081 cgcatgaaca tgcctgcatg gcgtttctct accgtggttt ttatactgag aaagtcggct
     1141 ccaaactgct cgaatgccct caacacagtc tgctgattcg tccttcaggg ttcgaacaca
     1201 gcaacgatta ccagcgaacc ggcgctgctt caatcatcat tgaagtcaaa caatcctggt
     1261 tgcaccaatt tgagcaacaa tccacgttgt ttcaggatgc cagcgttgtc caacacccgg
     1321 ttttggtgcc gacgtttcag cggatgtatg acgagtttca ttcaccacaa ccgctctcag
     1381 atttgtatct cgaagggctg attttagaag tactggtcca atccacccgg cttcagtcca
     1441 atctgcctga atcaggccaa cctttgtggt taaaggccgt tgtcgaattg atcgaagacg
     1501 acttttcccg cgacctgtcc ctggccgaaa ttgccaccgt ggcgggtgtt catccctcac
     1561 atttgaccag agtcttccgg caacagttcc actgctcgat tggcgaatat gtgcgccggg
     1621 tacgaatcac ctttgccaaa caccagcttg ccgaaacaga ttgttcgctg gctgaaattg
     1681 cgatggccgc cggttttttt gatcaaagcc atttctcaaa tgtgtttcga actatagctg
     1741 gtcaatctcc cttgaaatac cgacaagcca accgcaaatg ctcatccact ccaaaaatga
     1801 gctgaatttc caagaattct tgattgcact ctcatataaa ccacaccttt ccagaaggtt
     1861 gatgtcatca atcaccttct gccctggaga ttttttcatg acgacagacc agataaaatc
     1921 cgttcttggc tcgctggcga ggtttcccga gcaatttcgc cag
//
LOCUS       DAOQDO010001304         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58116_length_1961_cov_3.791710, whole genome shotgun sequence.
ACCESSION   DAOQDO010001304 DAOQDO010000000
VERSION     DAOQDO010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58116_length_1961_cov_3.791710"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1547
                     /locus_tag="PKZ53_18300"
     CDS             174..1547
                     /locus_tag="PKZ53_18300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNJ42447.1"
                     /translation="MLKKTRFLDPGRVFLAVGFSLCLFSNTVWAQQQGYPNPTTPQPL
                     ASGENVQGGVLTPPSLLDLVSSRISEYSRTDLAHGLYFETLDGKPLLETNPDAAYNPA
                     SVTKIMTTLAVLERYSPNYRYTTRLSYTGSFDRERQTLVGDLVIESNYDPTLNYDSLF
                     YIAEKLNEAGISTVEGNILVSSGLILNLRKSGVAAGNEILTTLDKARWTKASESAWKY
                     YLQAKAAAKMKIDSTEFRGVTFVSGKVLADDVSQPRKRLMEYHSAPILKVIKVMNAYS
                     NNDMAHLLGRLVGGPSGVQDFMIRRVKLAPGEIRLESTSGLGSNAITPRGTVKLMRYA
                     HNWCEKNRIAMTDLMPIGGVDNGTLADRFKRSDLVGSVVAKTGTLSNVSALAGVMFTK
                     ARGPVFFAILERGYPGSMRRLQEEIISLVAADSGGGLGIAYGSEMGMSLVEDARVYIL
                     REETSRN"
     gene            complement(1700..>1961)
                     /locus_tag="PKZ53_18305"
     CDS             complement(1700..>1961)
                     /locus_tag="PKZ53_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ42448.1"
                     /translation="VMALFSAQLDKIKKEGKEPDEVLKAEMEKVRATYEEQLDAKFAA
                     ARGFADAVIAPEDTRSALELALRTTLNNPGSHLGPFVLPDVR"
BASE COUNT          498 a          519 c          514 g          430 t
ORIGIN      
        1 accaagcacc aagcacgaag tgccaagcac caggcacgaa gtaccaagta ccaagcacga
       61 agtaccaagt accaagcacg aagtaccaag caccaagcac gaagtaccaa gtactaagta
      121 ccaggcacca ggcactaacc actaaaattc ttccttgaaa gctgagggac cttatgttga
      181 aaaaaacccg attccttgat ccagggcgcg tctttttagc agtgggattt agcttgtgtt
      241 tgttctcaaa cactgtgtgg gcgcaacaac aggggtaccc caatccgacg accccacaac
      301 cactggcgtc tggtgaaaat gtccagggtg gtgttctgac acctccgagc ctgctggatc
      361 tggtcagcag tcgaatcagt gaatattcac gcaccgatct ggcgcatgga ctgtactttg
      421 aaaccctgga tggcaagcca ttgcttgaaa ccaaccccga tgccgcctac aacccggcct
      481 cggtgaccaa aatcatgacc acactggcgg tgctcgaacg ctacagcccc aactaccggt
      541 ataccacccg tctgagctat accggaagct ttgatcggga gcgccagaca ctggtgggag
      601 atctggtaat tgaaagcaat tatgatccga cgctcaatta tgacagtctg ttctatattg
      661 cggaaaaatt gaatgaagcc ggaatttcaa ccgttgaggg aaatatcctg gtcagcagcg
      721 gtctgatcct caacctgcgc aagagcggtg tggccgccgg aaatgaaatt ctgaccacgc
      781 ttgataaagc ccggtggacc aaagccagtg aaagtgcctg gaagtactat ttgcaggcca
      841 aagccgccgc gaaaatgaaa atagactcga ctgagtttcg cggagtcacg tttgtttcag
      901 gcaaagtact ggccgatgat gtcagtcaac cacgcaaacg gttgatggaa taccattcag
      961 cgccgattct caaagtcatc aaagtcatga atgcctacag caacaacgat atggcccact
     1021 tgcttggccg attggttgga ggaccgtcag gagttcagga ttttatgatt cgacgcgtca
     1081 agctggcacc cggagaaatc cgcctggagt ccacgtctgg tcttggcagc aacgccatca
     1141 cgccacgcgg caccgttaag ctcatgcgct atgcccataa ctggtgcgaa aaaaatcgaa
     1201 ttgccatgac tgacctgatg ccaattggcg gtgtggataa tgggacactg gcagatcggt
     1261 tcaagcggtc tgatttggta ggaagcgtcg tggcgaaaac cggaacgctt tcaaatgtga
     1321 gcgcactggc aggggtgatg tttaccaaag ctcggggccc ggttttcttc gccatcctgg
     1381 aacgtggtta ccctggctca atgcgtcgct tgcaggaaga aatcattagc ctggtcgcgg
     1441 ctgatagcgg cggcggcctt ggaattgcct atggttcgga aatggggatg tcgctggtgg
     1501 aagatgcccg agtctacatt ctgcgtgaag aaaccagccg gaattagggc tgaagacatc
     1561 gggcttgggg ctgaagaaaa cgggttccat accctgagcc caatgtcttt agcccgaagt
     1621 tttcagtccg aagttttcag cccaatgtct tcagcccgat gtcttcagcc cgaagttttc
     1681 agccctgagc cctggttttt cagcggacgt ccggcaggac aaacggcccc agatgtgaac
     1741 cggggttgtt gagcgtcgtc cgcaacgcaa gttcaagtgc ggaacgggta tcttcaggtg
     1801 cgatcaccgc atcagcaaac ccacgagcgg cagcaaactt ggcatctaac tgctcttcat
     1861 acgtggcccg tactttctcc atttcagctt tcaatacctc gtcgggctcc ttcccttcct
     1921 tcttgatttt gtccagttga gctgaaaaga gcgccatcac a
//
LOCUS       DAOQDO010001305         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58125_length_1961_cov_3.030430, whole genome shotgun sequence.
ACCESSION   DAOQDO010001305 DAOQDO010000000
VERSION     DAOQDO010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58125_length_1961_cov_3.030430"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..538)
                     /gene="moaC"
                     /locus_tag="PKZ53_18310"
     CDS             complement(38..538)
                     /gene="moaC"
                     /locus_tag="PKZ53_18310"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNJ42449.1"
                     /translation="MSKDNSTPLEFSHLDDQGQLRMVDVSEKKVTDRTAVASGKVRMQ
                     ATTIAAIREHRTPKGDPLETARLAGILAAKQTDRLIPLCHGLPLTHVDVQITLAEDTV
                     ELRATARTQARTGVEMEALTAVSIAALTIYDMCKAVDKTMHITDIVLEKKTGGRSDLD
                     RMKNEE"
     gene            complement(525..1199)
                     /locus_tag="PKZ53_18315"
     CDS             complement(525..1199)
                     /locus_tag="PKZ53_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNJ42450.1"
                     /translation="MKVLPKPRVKICCINSVHEAHLAVRYGAAALGLVSEMPSGPGVI
                     SEAEIAQIAAAAPPAVGTFLLTSRQDTAAIIAQQRRCRVNTIQICDRLETGTYDELRA
                     ALPGISIIQVIHVTGPESIDEALAIAPHVHGLLLDSGNQSLAVKELGGTGRVHDWELS
                     RTICELAEKPVFLAGGLSPDNVCDAIEQVRPYGLDLCSSVRTNGQLDEHKLALFFKKV
                     NSYVEG"
     gene            complement(1379..1924)
                     /locus_tag="PKZ53_18320"
     CDS             complement(1379..1924)
                     /locus_tag="PKZ53_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNJ42451.1"
                     /translation="MLFTVLLIALGLAMDAFAVSISNGVTRKYLTFWQAVEIALFFGG
                     FQALMPLLGWSLGLTVRTLVESFANWIAFFLLAAIGSKMIYESFQESDPEADGRPLTV
                     LVLLALAVATSLDALAVGISFAFIKFPILWPSLLIGVVTFVISLVGVYIGKVFGEHFE
                     SKAETVGGLVLIGIGLKILLF"
BASE COUNT          508 a          506 c          487 g          460 t
ORIGIN      
        1 atacattctt cattcttcat tcttcattct tcattcttca ttcttcattc ttcattctgt
       61 ccagatccga gcgtcctcca gtttttttct ctaacacaat atcggtgatg tgcatggttt
      121 tatcgacggc cttgcacatg tcatagatgg tcagggcagc aatcgagaca gcggtaagtg
      181 cttccatttc aacgccagtg cgggcctggg tacgggctgt ggcacggagt tcaaccgtgt
      241 cttccgcaag cgtaatctgc acatccacat gtgtgagcgg cagaccgtga cagagtggga
      301 tcaggcggtc agtctgtttg gcagcaagaa ttccggccag tcgtgcggtt tcaagcgggt
      361 caccttttgg tgtgcgatgc tcgcgaatgg ctgcaatggt tgtggcctgc atgcgaactt
      421 tcccgctggc caccgccgtt cggtcagtga cctttttttc actgacatcc accatccgaa
      481 gttgtccctg gtcatcaagg tggctaaatt caagcggtgt ggagttatcc ttcgacatag
      541 gaattcactt tcttaaaaaa gagagccagt ttgtgttcat caagctggcc gttggtccgg
      601 acgctgctac atagatccaa cccatacggg cggacctgtt cgatggcatc gcatacattg
      661 tcaggactga gaccacccgc cagaaaaacc ggtttttctg ccagttcaca aattgtccgg
      721 ctgagttccc agtcgtgaac ccgaccagtt ccgccaagtt ctttcacggc aaggctttga
      781 ttgcctgaat cgagcaacag gccgtgaacg tgtggggcaa tggcaagggc ttcgtcaatg
      841 gattcagggc cagtgacgtg aatgacctga ataattgaaa ttccagggag tgccgcgcga
      901 agttcgtcat aggtcccggt ctcaaggcgg tcacaaatct gaatggtgtt gacccggcag
      961 cggcgttgtt gggcaatgat ggcagccgta tcctggcggc tggtcagcaa gaaagtacca
     1021 acggcaggtg gagcggcggc ggcaatctgg gcaatttcgg cttcgctgat gacgccaggg
     1081 ccgctgggca tttccgacac cagtccgagg gctgccgcgc cataccgaac agccaggtgt
     1141 gcttcgtgca cactattgat gcaacagatt tttacacgag gtttaggaag gaccttcata
     1201 gaaaccatcc gtattcaaat tatgaattct gcagggctta tctaatacca tttcgcaatg
     1261 aaaccctcgt attagaaaac agtcaagtaa ttcaatcaaa tggagttagc aagctactga
     1321 ctacaaactg gtataactca atcaccagga gagaattcat tcatacttca tacttcccct
     1381 agaaaagcag aattttgagt ccaattccaa tcaataccag tccaccgacc gtttcggctt
     1441 tgctttcaaa gtgttcgcca aaaactttgc caatgtacac accaaccaat gaaatcacaa
     1501 aggtcacgac accgatcagg agcgatggcc agagaatagg aaatttgata aaggcaaagc
     1561 tgatgccgac ggccagggca tccagactgg tggccaccgc cagggccagc aagaccagaa
     1621 cggtcagggg gcgtccatct gcttcaggat cactttcctg aaatgattca tagatcattt
     1681 ttgatccgat ggccgccagc aaaaagaaag caatccagtt ggcaaaactt tccaccaggg
     1741 tccgcaccgt caaccccagt gaccagccaa gcagcggcat caatgcctga aaaccgccaa
     1801 aaaagagtgc aatttcaacc gcttgccaga aggttagata tttgcgggtt acaccgtttg
     1861 agattgacac ggcaaaagcg tccatcgcca gcccaagagc aatcaagaga actgtgaaca
     1921 acataagaga atgaaaagcg gtgtcaagcc accgcactcc a
//
LOCUS       DAOQDO010001306         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58209_length_1960_cov_2.118110, whole genome shotgun sequence.
ACCESSION   DAOQDO010001306 DAOQDO010000000
VERSION     DAOQDO010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58209_length_1960_cov_2.118110"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="PKZ53_18325"
     CDS             complement(<1..1888)
                     /locus_tag="PKZ53_18325"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ42452.1"
                     /translation="MLTKKIEADQTQGLELGLTSGNALIGSESPVGGSHISFPWLKTA
                     FRQTPIITATKTDALQNDVDTDGAADPGDRLRYTVVITNSGGGNATGVNFSDTIGAST
                     TLVAGSVVASPLAINDSYSCIGNVGINVTVGGGVIANDLNPLGIGTLTVSATSTAGLQ
                     GTVTVNTDGSFQFTPTAGFEGSTSFTYTLSNGSGLTDTGTVTINVSGMIWFVNNNAGV
                     NGDGRLNTPFNSLANFNSGAADDPGDNIFLYRQVATNYTGGITLLANQKLIGQGATAS
                     LAAITGITVPTFSNTLPSTGGTRPTLTHTANNVVVSTGNTIRGVDLQSTGGSALFGTS
                     FNNLNASENTVSATGGTAAINLDQASSTAAINLNFISISANGSTNGIILADTTGSFTV
                     TGDGTNNASGGTIQSTTSNGISLNNVQNISLTSMRIQDITGLSANGIFGTQVNNFAFS
                     NNTIQNVGNLTAGSNAHGIAFDFQNDLMSPTNNNVSGTVSITNSTFTSIVNRGVDLVN
                     HSGSMTSLTISGNSLTGKTGGSAGIMVAVGITGTATAPSITISSNTIVANSGFGSGGI
                     VVGVTTSDTNVSNQTPSATAVIQGNTISACDGNDILATVRSCNGTLNVRIQGNNCAAP
                     VTGVR"
BASE COUNT          486 a          488 c          471 g          515 t
ORIGIN      
        1 gccgaacccc agttactggt gccgcacaat tgtttccctg gattcgaaca tttaacgttc
       61 cattacagga tcttaccgtt gccaaaatat cattcccatc acaggcgcta atggtgttgc
      121 cctggattac agccgtagct gacggggtct ggtttgacac attggtatct gaagttgtca
      181 ccccaacgac aatccctcca ctgccaaaac cactattggc aacaattgtg ttgctggaaa
      241 ttgttatgga tggggccgtg gcagtccctg ttatccccac cgccaccatg attccagcac
      301 ttccacctgt cttcccagtc agactattgc ctgaaatagt cagtgaagtc attgatccac
      361 tatgatttac aaggtccaca cctcgattga cgatgctggt aaatgttgag tttgtaattg
      421 agactgtgcc gctgacgttg ttattggtcg gtgacattag atcattctgg aaatcaaagg
      481 cgatcccgtg ggcatttgac ccggcagtta aattgccaac attctgaatt gtgttattcg
      541 agaaagcgaa attgttgact tgagttccga aaatgccatt agctgaaagc cctgtgatgt
      601 cttgaatcct cattgaggtc agcgatatgt tttgcacatt attgagcgag atcccattgc
      661 tggtagtact ctgaattgtc ccgcctgaag cattatttgt accatctcca gttaccgtaa
      721 aacttccggt tgtgtcagcc aaaataattc cattggtcga gccgttggcc gaaatgctga
      781 taaaattgag attgatagct gcggtactgc ttgcttgatc aagattgatt gccgctgttc
      841 ccccggttgc gctgactgtg ttttcagaag cgttgaggtt gttgaaactc gttccaaaca
      901 gagctgaacc acctgtgctt tgaagatcca cgccgcgaat ggtatttcca gtgctgacca
      961 ctacgttatt ggctgtatgc gtcagggtcg gacgtgtacc gccagtcgaa ggtaaggtat
     1021 tgctgaatgt cgggacagtt atccctgtaa ttgccgccag tgaagcggtt gcaccttgac
     1081 caattagctt ttgatttgcg agcagggtga tgccgccagt gtagtttgtg gccacttgac
     1141 gatacaaaaa gatattgtca ccaggatcgt ctgccgcacc agaattgaaa ttggccagag
     1201 agttaaatgg ggtgttcaat cgcccatcgc cattgacgcc agcgttgttg ttaacaaacc
     1261 agatcattcc tgacacattg atggttacgg tgcctgtatc ggtcagacca gagccatttg
     1321 agagggtgta ggtaaatgag gttgagcctt caaaaccagc agtcggcgta aattgaaagc
     1381 taccatccgt gtttaccgta accgtcccct gcaacccggc ggtactggta gcagacacag
     1441 tgagcgtacc gattccgagc gggttcaggt cattcgcaat cacaccacca ccaactgtta
     1501 cattgatgcc aacatttcca atacaactgt aactgtcatt gatggcaagg ggtgaagcaa
     1561 ccaccgaccc ggcaaccaga gttgtgctgg caccgattgt gtctgaaaaa ttgacgcccg
     1621 ttgcgtttcc accaccactg tttgtaatta ccaccgtgta ccgcaaccgg tcaccagggt
     1681 cagccgcacc gtccgtatct acgtcatttt gcaaggcatc cgtcttggtc gccgtgatga
     1741 tcggcgtttg tcgaaacgcg gtttttaacc aggggaagga tatgtgagat ccgccaaccg
     1801 ggctctctga gccaatcaga gcatttcccg aagtcaaccc caactcaagc ccctgagtct
     1861 gatcagcttc tattttcttt gtaagcaaat gattcccaaa ggcaaaaaca cagagaaggc
     1921 cagcgagcag aacacccgaa acagacatcc gggacagtat
//
LOCUS       DAOQDO010001307         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58239_length_1959_cov_3.103992, whole genome shotgun sequence.
ACCESSION   DAOQDO010001307 DAOQDO010000000
VERSION     DAOQDO010001307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58239_length_1959_cov_3.103992"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..>1959)
                     /locus_tag="PKZ53_18330"
     CDS             complement(72..>1959)
                     /locus_tag="PKZ53_18330"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ42453.1"
                     /translation="SEVFIIPAKGGTPVNVSMHPDNDQSPEWSADGRTLAFTSRRPGT
                     DLEVYYVFLRKSDDEKSKEDLEEDEKKNAKPARPITPPSSPATPKSQVEHQQEPPDSS
                     GQSEEPGKMGEKPTPVKVEIDFEDIHKRLRRLTTLPGNKISFAIAQDSKTYVFTCDNQ
                     GSVDLYSVVRDGGTPQSITRGGQFPTNLRFAKEQKLFFLAQGGRINTVSIPPAPKSGG
                     RQLPAGLSGGGATPTPVAFQARVLLDRATLRREAFLEGWQNMNDGFYDPKFHGANWPA
                     CREKYLNWAMVASCDEDYYDVVRMMLGELNSSHQGIFPPVDISDQAPIQTGALGVIWD
                     ESYTGEGMRVVRVIANSPASKSISRLNPGDVVLSINGEPISMKTGVDQVLNDTVGERT
                     LLVVAKDSAQGEKREVVIRPVSGGEFGNLIYDDWVDSRRAMVDKLSNGKLGYLHIRGM
                     DQVSQDRFEQELYSEAHGKSGLIIDVRDNGGGSTADYLLAMINVKPHAYTTPRGSTGK
                     GYPQDRLPFYFWTKPTTLLCNQFSYSNAEIFSHAFKTTKRGKLIGKQTYGAVISTGAA
                     QLIDGSTLRMPFRGWYVVGTNLNEELNGAMPDITVDVLPGDEAAGRDRQIERAVQELM
                     TEVK"
BASE COUNT          431 a          553 c          486 g          489 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac cccgaacccc
       61 gaacccggaa tttatttcac ctcagtcatc aattcctgga ccgcccgttc gatctggcgg
      121 tcacgtccag cagcttcatc acctggcaac acatccacgg tgatgtcagg catggcgccg
      181 ttgagttctt cgttgagatt ggttccgaca acataccagc cacggaacgg catgcggagc
      241 gtcgaaccat caatcaactg ggctgccccg gtgctgatga cagcgccata ggtttgcttc
      301 ccaatcaatt tgccgcgttt ggttgtttta aacgcatgcg aaaagatttc agcattcgag
      361 taagaaaact ggttacacag caacgtggtt ggtttggtcc agaaatagaa tgggagccgg
      421 tcttgcggat accctttccc ggtacttccc cgtggcgtgg tgtaggcatg cggtttgaca
      481 ttgatcatcg ccagcaaata gtcagccgtt gaaccgccgc cattgtcgcg aacgtcaatg
      541 atgagccccg acttgccgtg ggcttcggaa tacagttcct gttcaaaccg atcctgactg
      601 acctgatcca tgccacgaat gtgcaaatat ccaagttttc cgtttgaaag tttgtcaacc
      661 atcgcccggc gactatcaac ccagtcgtca taaatcaggt ttccgaactc acctccgctg
      721 actggacgaa tgacaacctc tcgtttttct ccctgggcac tatctttggc caccaccagc
      781 aaggttcgct caccaaccgt gtcatttaac acttgatcta caccggtctt cattgaaatc
      841 ggctcgccat tgattgagag caccacatca cccggattga gtcggctgat ggattttgaa
      901 gctggtgagt tggcaatcac tcgcaccacc cgcatgcctt cgcccgtgta tgattcatcc
      961 cagatcaccc caagcgctcc ggtttgaatt ggtgcctggt cagaaatatc aactggcggg
     1021 aaaattccct ggtgcgaaga attcagttca cccagcatca tgcgcaccac gtcataataa
     1081 tcttcatcac atgaggcaac catagcccag ttgaggtact tttcacggca ggcaggccag
     1141 ttggcaccat ggaatttcgg gtcgtaaaac ccatcgttca tattttgcca gccctctaaa
     1201 aaggcttcgc gccggagtgt tgcgcgatcc agcagcactc gtgcctgaaa cgccaccggt
     1261 gtcggggtgg ccccgccgcc tgaaagaccg gctggcaact gacgtccacc tgattttggt
     1321 gcaggcggga tcgaaacggt attgatgcgc ccgccctgag ccaggaaaaa gagcttctgt
     1381 tcttttgcaa atctcagatt ggtcgggaat tgtccacctc gggttatgct ttgcggcgtt
     1441 ccgccatccc tgaccactga atagaggtca actgacccct gattgtcaca ggtaaacaca
     1501 taagttttgc tatcctgggc aatcgcaaaa gaaattttgt ttcctggaag tgtggttaac
     1561 cgccgcaagc gtttatggat gtcttcaaag tcaatttcga ctttgaccgg cgtcggtttt
     1621 tcacccattt tgccgggctc ttctgactga ccagaagaat caggtggctc ctgctggtgt
     1681 tcgacctgtg attttggcgt agcgggtgag gatggcggcg ttatcggacg ggctggcttg
     1741 gcgtttttct tttcatcttc ttccaggtcc tctttgcttt tttcatcgtc gctcttgcgc
     1801 aaaaacacat agtacacttc gagatcagtt ccaggtcgcc gcgacgtaaa cgccagcgtc
     1861 cgtccatcgg ctgaccactc aggtgactgg tcattatctg ggtgcattga cacattgacc
     1921 ggggttccac ctttggccgg aatgataaag acctcgctg
//
LOCUS       DAOQDO010001308         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58243_length_1959_cov_2.890231, whole genome shotgun sequence.
ACCESSION   DAOQDO010001308 DAOQDO010000000
VERSION     DAOQDO010001308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58243_length_1959_cov_2.890231"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1231)
                     /locus_tag="PKZ53_18335"
     CDS             complement(92..1231)
                     /locus_tag="PKZ53_18335"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ42454.1"
                     /translation="MQCPTCGIVNRDDAKFCTRCGSTLTPAGGARGAKYRQPGMMLRD
                     MGADETLTGEAPVSAEPSNVVTVSSEAIPPGAPFIEVVFLTGLRKGEKLVLSTFPATV
                     GRDPGSMLQMDLNDTLASTRHAHLLFEGNRFILRDIGSTNGTYHKGNRVKEIVLTHGD
                     VIEFGIGGPKLRFDMPWMVATPPPMHVDSFSGPAIPGAMITPKAERAPGPVLLQKDQN
                     RMPETPRGNDIPAWARNAPPPPELITPRPQQLDSSSQPGSPQFLNRPIPEPQSETPFI
                     PSTVDQGSSQAGLVTGGNRAAVSHPGGDQPQSVPDQNASAQKRRLMAVIKLILGFGLL
                     ILGAVVAIVFKSQPLLVGFMACVMVGGIAFAIWGAAQLILGGSKQ"
     gene            1565..1942
                     /locus_tag="PKZ53_18340"
     CDS             1565..1942
                     /locus_tag="PKZ53_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42455.1"
                     /translation="MTTSSHFGYQGSAIRNPFISTSLLILIFVAGLWVTPVDVAAQET
                     QKPRVLSKGRTKSRGDRPPRGIKSEFGESGRSAGRGGKGLGKNIAKGRVLRAGKEFGK
                     GMGGFGKHLGKGVGKSVKHAFKP"
BASE COUNT          463 a          492 c          517 g          487 t
ORIGIN      
        1 tggtgcttgg ttcttggtgc ttggtgcttg gtgcttggtt cttggttctg cgaaaggatt
       61 ggctcgcaag taccaagtac caggccccgc cttactgctt tgagccgcca aggatcaact
      121 gggcagcccc ccaaatggca aaggcaattc cccctaccat gacacaagcc ataaatccaa
      181 ccaggagggg ctgacttttg aacacaattg cgactaccgc tccgagaatt aataacccaa
      241 accccagtat caacttgatg acggccatca agcgacgttt ctgggcactg gcgttttgat
      301 ctggtacaga ttgcggctgg tctccacctg gatgagatac tgctgcccga ttgccgccag
      361 ttaccagtcc agcctgggaa cttccctggt caacggttga tggaataaac ggcgtttcac
      421 tctgtggttc tgggatgggg cgatttaaaa attgcggact gcctggctga gaggatgaat
      481 caagttgttg aggtctgggg gtaatcaatt ctggcggagg tggtgcgttg cgtgcccagg
      541 caggaatatc atttccccga ggcgtttccg gcattcggtt ttgatctttt tgcagcaaga
      601 ccggccccgg agcacgttca gctttcggcg ttatcatggc gccaggaatg gcggggccgg
      661 aaaacgaatc aacgtgcatt ggcgggggcg tggcaaccat ccagggcata tcaaagcgga
      721 gcttcggacc accaatgcca aactcaatca catctccgtg agtgagcacg atttccttga
      781 cccggttgcc tttatgatag gtcccattgg tgctgccaat atcgcgtaaa ataaagcggt
      841 ttccttcaaa gagcaaatgg gcgtgtcgcg ttgaagccag cgtgtcattc aaatccattt
      901 gcaacatgct gcctgggtca cgtccaacag ttgccgggaa ggtggaaaga accagttttt
      961 cacccttgcg cagtccagtc aaaaacacga cttcaataaa cggcgctcca ggtggaatag
     1021 cttcagaact cacagtgact acgtttgagg gctcagccga aactggggct tcacccgtta
     1081 aggtctcgtc ggcgcccata tcccggagca tcattccggg ttggcggtat ttcgctccac
     1141 gcgcccctcc tgccggagtg agtgttgaac cacagcgggt acaaaacttt gcatcatctc
     1201 gattgacgat accgcaggtt ggacattgca taacgcacat tcctaaatgg ggtatagatt
     1261 tccacacggg ttgccgcaag tctaacacgg tcaacagaca tttcaagata cggccaaaat
     1321 ttcaatgggt ggtccagatt tctgcagcaa ttcaacgtca gattcagggc aaccctgtgc
     1381 taggatttcg cttctgctgg ataaatccat ctcttttttt caaatcactt tttcatccgg
     1441 gcttttagtc agtagtcagt ggtcagtagt tttctaaagt tgtttacttg aattacttag
     1501 ctgtctgcct gccggaagcg atgattttat ttttgtatca attctcagat aggagccaac
     1561 acatatgaca acttcttcac attttgggta tcaaggttcc gcgattcgaa atccatttat
     1621 ttccaccagt ttactgattc tgattttcgt agccggtctc tgggtcactc cagtggatgt
     1681 cgccgcacag gaaactcaaa aaccgcgagt tctatccaaa ggacgcacta aaagccgtgg
     1741 agaccggcct ccgcgcggaa tcaaaagcga atttggcgaa tctggccgct cagccggacg
     1801 cggtggcaag ggcttaggga aaaatatcgc caaaggccgg gttctgcgcg ccggcaaaga
     1861 atttggcaaa ggcatgggtg gctttggcaa acatcttggc aaaggcgtcg gcaaaagcgt
     1921 caagcatgcc tttaaaccgt agtggggctg agggctggg
//
LOCUS       DAOQDO010001309         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58318_length_1958_cov_2.510247, whole genome shotgun sequence.
ACCESSION   DAOQDO010001309 DAOQDO010000000
VERSION     DAOQDO010001309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58318_length_1958_cov_2.510247"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1958
                     /locus_tag="PKZ53_18345"
     CDS             <1..>1958
                     /locus_tag="PKZ53_18345"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42456.1"
                     /translation="AIREKVFGPDHPSVARGLSNLGLLYRNKGDVENAEIFFRRSLAI
                     REKTLGPDHPEVANLYSFLAGLYRHRGDYQQAESFFLRDLAISEKALGADHPRVASTL
                     NNLAALYRVKGDISKAIAYQVRANNATERDLLRNLVTGSENQKMLYLETTNYRTNQTI
                     SLHLQSAPQDQAALQAAITVILRRKGRLLDAASQGIEVLRQRATAEDQKLLDELTEAK
                     SELSRLTLRGPGKEGIERHRAFLKELDAKADQLEAQVNARSAEYRSLVQPITLEAVQQ
                     AIPADAALIEFALYQPFDVQTRTYGNWRYVAYVVPGSGFRVPGSGSESGVIQKSKPTQ
                     RNRNQRAQVPLAKVEPGTRNPEPGTLNWVDLGEAGPIDQVVTAFRTVLSDSKKDIAHQ
                     VLPVAQVLDSLVMKPVRKLIGPKRHLLISPDGMLNLIPFSALVDEQGTYLVENYTLTY
                     LTSGRDLLRLKVKVPGAEPAVVIANPEYATGSGPVLAGQTLKPLVPLPGTEREGREIQ
                     KILGVQTQLFVQTKATKDVVTRIHRPGVLHIATHGYFPADTLREVSDLENRQSGESEA
                     EAIAKPTIENPLLRSWLFFASANRGTENSNGVLTALEASQLDLWGTKLVVLSACDTGL
                     GEVKNGDGVYGLRRALVLAGSEAQMMSL"
BASE COUNT          488 a          500 c          541 g          429 t
ORIGIN      
        1 ggcgattcgt gaaaaagtgt ttggtccaga ccacccgtcg gtggcccgtg gcctgagcaa
       61 tctggggttg ctctatcgaa acaaaggcga tgtggaaaac gcagagattt tcttccgccg
      121 gtcactggcc atcagggaaa aaacactggg tcccgatcac ccagaggtgg ccaatctgta
      181 cagctttctg gcggggcttt accgacatcg gggtgactac cagcaggctg aatccttttt
      241 tctgcgtgat ctggccatca gtgagaaggc cctgggggct gatcatcccc gtgtggccag
      301 caccctcaac aatttagcgg cactgtatcg agtcaaaggg gatatatcga aagcgattgc
      361 ttatcaggtt cgtgccaaca atgccaccga gcgcgatttg ctcaggaatc tggttaccgg
      421 ttcggaaaac cagaaaatgt tatatttgga aacgaccaat taccgaacca atcagacgat
      481 ctccctgcac ctccaatcag cgccacagga tcaggctgca ctccaggcgg caataacggt
      541 catcttgcgg cggaaaggcc ggttactgga tgccgcaagc caggggattg aagttttacg
      601 ccagcgagcc acggctgaag atcaaaagct gctcgatgaa ctgaccgaag ccaaatctga
      661 actttcgcgt ctgacactcc gtggaccagg aaaagaggga attgaacgcc atcgggcatt
      721 tttgaaagag cttgacgcga aagctgatca actcgaagcc caggtcaatg cccggagtgc
      781 tgaatatcgc tcactggtgc agccgattac cctggaggcc gttcagcaag ccattccagc
      841 cgatgccgcg ttgatcgaat ttgctctcta ccagccgttt gacgtgcaaa cccgaaccta
      901 tggcaactgg cggtatgtgg cgtatgttgt tccgggttcc gggttccggg ttccgggttc
      961 cgggtctgaa tcaggggtaa ttcagaaatc aaaaccaaca caacgaaacc gaaatcagag
     1021 ggcacaggtg ccactggcta aggtggaacc cggaacccgg aacccggaac ccggaaccct
     1081 gaactgggtt gacctcgggg aagccgggcc aattgaccag gtggtaaccg cttttcgaac
     1141 ggttttgagc gattccaaaa aagatatcgc tcaccaggtt ctgcctgttg cccaggtgct
     1201 cgattcattg gtgatgaagc cagttcgaaa attgattggt ccgaaacgcc atctgctgat
     1261 ctcaccagat ggaatgttga acctgattcc tttttcggct ctggtggatg aacagggaac
     1321 atatctggtt gaaaactaca cgttgaccta cctgaccagc gggcgtgatt tattgcggtt
     1381 aaaggtgaaa gtccccggtg ctgagcctgc cgttgtcatc gccaatccgg agtatgcgac
     1441 tggcagcggt ccagttctgg ccggtcagac cttaaaaccg cttgtaccac ttcccggaac
     1501 agagcgagag ggacgagaga ttcagaaaat actgggtgtt caaacacagt tgtttgtcca
     1561 aaccaaagca acaaaggatg ttgttacacg cattcatcgc cctggagtat tgcacattgc
     1621 cacccacggg tatttcccag ccgatacgct ccgggaagta tcagacctgg aaaatcgcca
     1681 atcaggcgaa tctgaggctg aagcgattgc aaaaccaaca attgaaaacc cactcctgag
     1741 atcgtggctc ttttttgcga gcgccaatcg tggaactgaa aattccaatg gggttctgac
     1801 agcacttgaa gcctcgcaac ttgatctctg gggcaccaaa ctggtggtgc tttcggcctg
     1861 tgatacgggc cttggcgaag tgaaaaatgg agatggggtg tatggattgc gccgggcact
     1921 ggttctggct gggagtgaag cccagatgat gagcctct
//
LOCUS       DAOQDO010001310         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58375_length_1957_cov_2.515773, whole genome shotgun sequence.
ACCESSION   DAOQDO010001310 DAOQDO010000000
VERSION     DAOQDO010001310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58375_length_1957_cov_2.515773"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PKZ53_18350"
     CDS             complement(<1..515)
                     /locus_tag="PKZ53_18350"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNJ42457.1"
                     /translation="MTTHFRLRVMLVKWSLFFFWVLAFNIPIFSQTQPAKETPKPATF
                     RVVTKPVAPFVISDTNPVSGFSIDLWKAIGDEMGIKSELIMKPTVTDLLKTVKAGEAD
                     LGIAAVSITAQREQDFDFSYPVFDSGLQILVPVGNTAQFSLKDTLASWVSVALLKILG
                     VLALLIVFLGH"
     gene            785..>1957
                     /gene="guaA"
                     /locus_tag="PKZ53_18355"
     CDS             785..>1957
                     /gene="guaA"
                     /locus_tag="PKZ53_18355"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNJ42458.1"
                     /translation="MLHTETVLILDFGSQYTQLIGRRVRELNIYCEILPFHTSLEAIQ
                     AKNPRAIILSGGPSSVYGVGAPHCSPDLLNLGVPVLGICYGMQILASFLDGKVESSNR
                     REFGAADIQVETDSRLFAGLPRQFPVWMSHGDHVTAAPSGFTVSATSSNALGAMEDPR
                     RGLYAIQFHPEVVHTPHGKDILRNFLVEIAGINPNWTMRGFIEQAVEAIREQVGDGHA
                     VCGLSGGVDSTVAAVLVSKAIGHRLTCLFVDNGVLRLNEYEDVLETFRTGLHLNVRGI
                     PASDRFLSALAGVSDPEQKRKIIGREFIEVFQDEAQKLGKIDFPVQGTLYPDVIESVS
                     VKGPSAVIKSHHNVGGLPEKMHLKLIEPLRELFKDEVRAVGRELGLPEALITRQPFP"
BASE COUNT          455 a          495 c          507 g          500 t
ORIGIN      
        1 atatgaccga gaaaaacaat cagcaacgcc aggacaccga gaatttttaa gagcgccact
       61 gatacccagg aggcaagggt gtctttcaac gaaaactgag cggtgtttcc tactggaacg
      121 agaatctgta accctgagtc aaaaaccggg taggaaaaat caaaatcctg ttcgcgctga
      181 gccgtaattg acacggctgc aattccaagg tcggcttctc cggctttgac ggttttgagc
      241 aggtccgtca ccgtcggttt cataattaat tcggatttga tccccatttc atcgccaatc
      301 gccttccata aatcaatgct gaagccggaa acgggattgg tgtctgaaat gacaaacggt
      361 gcgactggtt tggtaacaac ccgaaaggtg gctggtttgg gcgtctcttt ggctggttgg
      421 gtctgggaaa agattgggat gttgaacgcc agtacccaaa aaaagaataa actccatttc
      481 acaagcatca cgcgcagtcg aaagtgggtg gtcatgccct ggtttggcgc attttttcca
      541 atgctgcttt gatgaaacct ttcagctcgg tgtgatcaat gcaccgttct cctttttgaa
      601 gattgaatac tttcattgaa gcttttacgg acattttgac ggatggtcaa ctggataatc
      661 attcactgga aaactggtgc ttgaaccatt ccagtgtcac cctccaccgg ttcgcctttg
      721 catcgccctg gttggaagtg gtaaaagcca ggttcctcca atatttccca aaggtgaact
      781 cttcatgctg catactgaaa ccgttctcat tcttgatttt ggttcgcaat acacccagtt
      841 gattggccgg cgggtgcgcg aactcaacat ttactgcgaa atccttccgt ttcatacctc
      901 acttgaagcc atccaggcca aaaatccacg tgccatcatc ctctccggtg gcccgtcatc
      961 ggtctatggc gttggtgcac cacattgcag cccagacctg ctgaacctgg gtgttccagt
     1021 gctgggcatt tgttatggca tgcagattct ggcctctttt ctggatggca aagttgaatc
     1081 ctccaaccgg cgcgaatttg gcgcggctga catccaggtt gaaaccgata gccgactctt
     1141 tgccgggttg ccgcgccagt tcccagtctg gatgagccac ggcgaccacg tcacggcagc
     1201 gccgtcaggt tttaccgttt ccgccaccag ttccaacgca ctaggggcaa tggaggaccc
     1261 acgccgtggc ctgtatgcca tccagtttca cccggaagtc gtccacacgc ctcacggaaa
     1321 agacattttg cggaattttc tggtggagat tgccggtatc aatccgaact ggaccatgcg
     1381 tggctttatc gagcaggccg ttgaagccat ccgcgagcag gtcggcgatg gtcacgccgt
     1441 ctgtggtttg tcaggtggcg tagactcaac tgtggctgcg gtcctggttt cgaaagcgat
     1501 tggtcaccgg ttaacctgcc tgtttgttga caatggtgtg ctgcgcttaa acgaatatga
     1561 ggatgtcctg gaaactttcc ggacggggct ccacctcaac gtgcgtggca ttccagccag
     1621 tgatcggttt ttatcagccc tggctggtgt ttccgacccg gaacagaagc gaaaaatcat
     1681 tggccgcgaa tttatcgaag tcttccagga tgaagcccag aagcttggca aaattgattt
     1741 tccggttcag ggaacgctct atcccgatgt gattgaatca gtttcggtga aaggtccttc
     1801 tgcggtgatc aaaagccatc acaacgttgg tggactacca gagaaaatgc acttgaagtt
     1861 gattgagcca ctgcgtgaat tgtttaaaga cgaagtgcgc gctgtaggac gggagttagg
     1921 cctccctgag gcgttaatca cacggcaacc atttcct
//
LOCUS       DAOQDO010001311         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58378_length_1957_cov_2.448475, whole genome shotgun sequence.
ACCESSION   DAOQDO010001311 DAOQDO010000000
VERSION     DAOQDO010001311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58378_length_1957_cov_2.448475"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="PKZ53_18360"
     CDS             <1..1773
                     /locus_tag="PKZ53_18360"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNJ42459.1"
                     /translation="DLNEATKRLPRLRITTFRDELGRSLENLPLRVQPETFGAVRILE
                     ATDVRGLTFRAVFVLGLVEGGFPIRGRADWIYPPAEREQLKEYGLTLEDISPAVMRKE
                     EHYFYQIVNRATERLYVSFPKNQEDDTEAVKSYFLTELERVYPAGLFELEPAPDTISL
                     ARPVPKGYDGSQILESTTVRELARASTALRRRLKLGESPDDFAISVGPLTSAEVVTIL
                     EQYTFDRGYVSKLAQRRLEIEAERYGSRFGTFDGRMESDIVRSAVANQFGEDRVYSAT
                     EFNEYGQCPFRFFAHRVLDLRPRVEAALDLQNQDRGLLLHEILREFFSRLRGKSLSEI
                     GREKLLIQLKKTAEDVFNNFETRLPPLNPKLWEIERSVLILQLEQVVDYEMEFEDSAA
                     RPVRPRFGELAFGMSPRHRDPASLREPLLLTSPNGEIMRLRGQIDRVDVSEDGKLVAY
                     DYKTSRGASINDMRAGRDVQMAIYLEALERLFCREEQEVIGGGYYSLRPGSDRRNNGL
                     YRADFSAYTGLGRTASNLSDDDWQATRKQLINYVWRYWHHLRDGDFRVRPAQNELTCQ
                     YCDYKAVCRFDRYRIQKKKKVSRQ"
BASE COUNT          462 a          499 c          528 g          468 t
ORIGIN      
        1 gacctaaatg aagccaccaa acgacttccg cgtttgcgga tcacaacatt tcgcgacgaa
       61 ctggggcggt cgctggaaaa tctgccgctc cgggtgcaac ctgaaacctt tggggcggtg
      121 cggattctcg aagccactga tgttcgcgga ctcacgtttc gggccgtgtt tgtgcttggg
      181 ctggttgaag gcgggtttcc gattcgtggt cgcgctgact ggatttatcc tccggcagaa
      241 cgcgaacagt tgaaggaata cggcctcaca ctcgaagaca tttcgcccgc cgtgatgcgc
      301 aaggaagaac actatttcta tcaaatcgtc aaccgcgcga cagagcggct ctatgtatca
      361 ttcccaaaaa accaggaaga tgacaccgaa gccgtaaaat cctattttct cactgagtta
      421 gagcgggtgt atccggctgg cctgtttgag cttgaaccag cgccagacac catctcactg
      481 gcccggcctg tgccaaaagg ctatgacggt agccagattc tggaatccac cacggttcgc
      541 gaactggcgc gggccagtac ggcactccga cggcggctca aactgggcga atcacctgat
      601 gattttgcga tttccgttgg cccacttact tccgctgaag tggtcaccat actggagcaa
      661 tacacctttg atcggggcta cgtgtcaaaa ctggcacaac gacggcttga aatcgaagct
      721 gaacggtatg gttcgcgatt tgggaccttt gacggcagga tggaaagcga cattgtgcgc
      781 agtgcagtgg ccaaccagtt tggcgaagat cgggtataca gtgccaccga gttcaacgaa
      841 tacggccagt gcccgtttcg cttctttgca catcgagtgc tggacctgag acctcgggtt
      901 gaagccgccc tggatttgca gaaccaggat cgcgggttgt tgctccacga aattttgcgc
      961 gagtttttca gccgactgcg cgggaaatcg ctctctgaaa tcgggcgtga gaaattactg
     1021 attcaactga agaaaaccgc cgaagatgtt ttcaataact ttgaaacccg gttgccacca
     1081 ctgaacccaa agctttggga aatcgaacgg tcggtgctca ttcttcaact tgagcaagtg
     1141 gttgattatg aaatggaatt tgaagactca gcagcgcgtc cggttcgacc acgctttggc
     1201 gaactcgcgt ttgggatgtc gccccggcat cgtgacccgg catcgctccg ggaaccactt
     1261 ttgctgacga gtccaaacgg tgaaatcatg cggctgcgcg gacaaattga ccgggtggat
     1321 gtctcagaag atggcaaact cgtcgcctat gactacaaaa cctcacgcgg ggcgtcaatc
     1381 aacgacatgc gggctggtcg tgatgtgcag atggcgatct atcttgaagc gctggaacga
     1441 cttttctgcc gcgaagaaca ggaagtcatc ggcggcgggt attattcgtt gcgtccaggt
     1501 tcggatcgcc gcaacaatgg actctaccgg gctgattttt cagcctatac cggcctgggt
     1561 cggacggctt caaatctgag cgatgacgac tggcaggcca cccgcaaaca acttatcaat
     1621 tatgtctgga gatactggca tcacctgcgc gatggcgatt tccgggtccg cccagcccaa
     1681 aacgaactca cctgccagta ttgcgattat aaggctgtct gccgatttga ccggtatcgg
     1741 attcagaaga agaagaaagt cagtcgtcag tagtcagtag tcagttgtca gttgtcgagt
     1801 gttggggttt tgaattgtct gtcaagtcta tttggttcta tttgagtcag ttatgaataa
     1861 ttcttttcaa gaactttaca ggttgcaata tccaggtttt atctactgac tactgactca
     1921 tcaactactg actcatcaac tactgactac tgactca
//
LOCUS       DAOQDO010001312         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58416_length_1956_cov_3.025776, whole genome shotgun sequence.
ACCESSION   DAOQDO010001312 DAOQDO010000000
VERSION     DAOQDO010001312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58416_length_1956_cov_3.025776"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..971)
                     /locus_tag="PKZ53_18365"
     CDS             complement(66..971)
                     /locus_tag="PKZ53_18365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ42460.1"
                     /translation="MPALSPMEAEQLKAQVRDFWNHNPCGLKFANVEFGTREFFDAIE
                     AHRYATEGHIKEVVDFPRWKGKKVLEIGCGLGTDALQFARAGADYLGVDLTETAAQTT
                     ERRLRMYGVPGRTQIADAERLPFADNTFDLVYSHGVLHHTPDTEQAIREVHRVLRPGG
                     TAMVMLYHRNSYNYYGNILFFRRLGAQLFRFSWGPKLANLLTGEDLDRLKELQQRMKQ
                     DPQRFFSTEEFLNQNTDGVGNPLARVYSRAEVCRIFSMFSQVKTEVHFLNKRWLPLIG
                     PLLPRFIELPLAHRIGWHLWIFGQK"
     gene            complement(1029..1238)
                     /locus_tag="PKZ53_18370"
     CDS             complement(1029..1238)
                     /locus_tag="PKZ53_18370"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNJ42461.1"
                     /translation="MTSAHLDLNQQLIPILNQLNQPLPEAVNELIVLELYRRGVLSSG
                     KAGEFLAMPRFEFIHFASRLGDCLL"
     gene            complement(1235..>1956)
                     /locus_tag="PKZ53_18375"
     CDS             complement(1235..>1956)
                     /locus_tag="PKZ53_18375"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ42462.1"
                     /translation="TRNPEPGTRNPEPGTRNSNMPAPTQTPLQTEQLVVLSNVTWATY
                     KQLSADLGDNRATHLAFLHGNLEIMSPSRLHEQLAQFLTRIVILLAESFKQPIEACGA
                     MRLEREDVAISAEPDGSFYLANEPRIRRKVALDFTVDPAPDLIIEIDISSGSLAKFAI
                     YETFRIPEIWRYDERKGFMIYVLSEAGYAPTEQSSTFTEITRNEIQTLISECQKNGQT
                     AAIKKFQSWLKLKKKQRKKSL"
BASE COUNT          461 a          496 c          504 g          495 t
ORIGIN      
        1 gggtgatttt tttcatccct catccttcat ccctcatccc ttccgaaacc cctgaaccct
       61 gcttttcact tttgcccaaa aatccacaaa tgccagccaa tacggtgggc cagcgggagt
      121 tcgataaagc gtggcaacaa cggcccaatc aacggaagcc agcgtttgtt gagaaaatga
      181 acctcggttt tgacctgcga aaacatcgag aagatccgac acacttccgc ccggctgtac
      241 acccgcgcca gcggatttcc gacgccgtca gtgttttggt ttaagaattc ctcagttgaa
      301 aaaaaccgct gtggatcctg tttcatccgt tgttgcagct cttttagccg gtcaaggtct
      361 tcgccggtga gaagattcgc cagtttcggc ccccaggaaa accgaaacaa ctgcgcgcct
      421 aaccgccgaa aaaacaggat gttgccatag tagttatatg aatttcggtg atagagcatc
      481 accatcgctg ttccaccagg gcgcaggaca cggtggactt cgcggatagc ctgttcggta
      541 tccggtgtgt ggtgcagcac gccgtgggaa tagaccagat caaaggtgtt gtcagcaaac
      601 gggaggcgtt cggcatcggc aatctgggtg cggcctggaa cgccatacat ccgcaggcgt
      661 cgttcggtgg tttgagcggc ggtttcggtg aggtctacgc caaggtaatc agctccggcg
      721 cgagcaaact ggagcgcatc cgtccccagc ccacagccaa tttcaaggac ttttttgccc
      781 ttccagcgcg ggaaatcaac gacttccttg atgtggcctt cggtcgcgta gcggtgggct
      841 tcaatcgcgt cgaaaaattc gcgggtgcca aattcgacat tggcaaattt gagtccgcag
      901 gggttgtggt tccagaagtc gcgaacctga gctttaagct gttcggcttc catcggtgaa
      961 agagcaggca taatcatcaa acgaaacgac tccgttccaa ttcagattcc cattcattgg
     1021 gtagcaaatc aaagtaagca atcccccaac cgtgaagcaa agtgaataaa ctcaaagcgg
     1081 ggcattgcta agaactcacc agctttcccg cttgaaagca ctccacggcg atataactcc
     1141 aatacaatca actcattgac cgcttctggc aatggctggt tcaattgatt aaggattggg
     1201 atcagttgct gattgaggtc aaggtgggct gaagtcatag ggatttcttt ctctgttttt
     1261 tcttcagttt gagccaggat tgaaactttt tgatggcagc cgtttggcca ttcttctgac
     1321 attcggaaat cagggtttga atctcattgc gcgtgatttc cgtgaaggtt gagctttgtt
     1381 cagttggcgc atatccagcc tccgaaagaa catagatcat gaagcccttt cgttcgtcat
     1441 agcgccagat ttctggaatg cggaacgttt cataaatggc aaacttggcc agcgagccac
     1501 tgctgatatc aatttcaatg atgagatcag gtgccgggtc aactgtaaaa tccaatgcta
     1561 ctttccggcg gatgcgtggt tcattcgcca gataaaacga tccgtctggt tctgccgaaa
     1621 ttgccacatc ctctcgttca agcctcattg cgccacaggc ttcaatcggt tgcttgaagc
     1681 tttccgccag taaaatgaca attcgagtga gaaactgggc cagttgttcg tgcaaccgcg
     1741 atggactcat aatttccaaa ttgccgtgaa gaaaagccag atgtgttgcc cgattatctc
     1801 ctaaatcagc actcaactgc ttgtaggtcg cccaggtcac attggagagg accaccagtt
     1861 gctcggtttg aagtggagtt tgggttggtg caggcatatt cgagttccgg gttccgggtt
     1921 ccgggttccg ggttccgggt tccgggttcc gggttc
//
LOCUS       DAOQDO010001313         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58417_length_1956_cov_3.025776, whole genome shotgun sequence.
ACCESSION   DAOQDO010001313 DAOQDO010000000
VERSION     DAOQDO010001313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58417_length_1956_cov_3.025776"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..815
                     /locus_tag="PKZ53_18380"
     CDS             288..815
                     /locus_tag="PKZ53_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42463.1"
                     /translation="MVTLVNIIQFMIKQQAAAIHEIKFANLVEAEVTVLAYVSPDGKS
                     FRIKRPKDPNQAALWTRKQVTYIKKTGHLTPKNPRWAEYQIAKRWVSVLLTARHLLKI
                     GNGDVLERDAFFQAVRSGQLCHHVRKTQHPRQMAIKAHRLLRKLKNRLEHTPERFAEV
                     EAWMQRIAEEGHRGA"
     gene            805..930
                     /locus_tag="PKZ53_18385"
     CDS             805..930
                     /locus_tag="PKZ53_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42464.1"
                     /translation="MEHKPNLKLYVLVRRDLSPSQVAVQACHAVATFVSRHGRDP"
     gene            1456..>1956
                     /locus_tag="PKZ53_18390"
     CDS             1456..>1956
                     /locus_tag="PKZ53_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42465.1"
                     /translation="MRRIIFSLLFAYLIVLTTIWIPTYSHTALLSKRNRALVATVRAQ
                     SAPTISSISPTQTAKGTAPTKIVIKGTGFQSGDVVKFNDTAVSAKIKSATKIAIKNAA
                     ASLFQTDVTYKVTITSSSGQSSNSLDFIVGTGSQTNTGEPASIVITSPTSFVLNTPNA
                     LTSNTVA"
BASE COUNT          505 a          502 c          461 g          488 t
ORIGIN      
        1 agggctgaac atacctgggc ctggggcttg gggttgaaga ctcgcaagct cggggcagaa
       61 gaaaatgggt tcaatgtcct gattccaacg tcttcagccc gatgtcttca accagttttc
      121 ttcaacccca agccctcagc ccgttttctt ccgccccaat tcttcaaaag gaggtttgcc
      181 atgttgtagc aagctcgtcc accgtgatgt gccattgtcg tgattttcct ggcctttgct
      241 tgaggctaac ttattctttt tcacaggttt cacgaaagga aacgacaatg gttactttag
      301 tcaatatcat ccaatttatg atcaaacagc aggctgcggc cattcacgaa atcaagtttg
      361 ccaatttagt tgaagcggaa gtgacggttc tggcttatgt gtcacctgac ggcaaatcat
      421 ttcgaatcaa acggcctaaa gatccaaatc aagcggcact ctggactcga aaacaggtga
      481 cttacattaa gaaaacaggt catctcacgc caaaaaatcc acgctgggcg gaataccaga
      541 ttgccaaacg ctgggtttcg gtgctgctca cggctcgcca tctgctgaaa atcgggaatg
      601 gcgatgtttt agagcgagat gcgttttttc aagccgtgcg aagcggccag ttatgccatc
      661 acgttcgcaa aacccagcat ccacgccaga tggcgatcaa agctcatcgg ttgctgcgca
      721 agctgaaaaa ccggctcgaa cacactccag aacggtttgc cgaagtcgaa gcctggatgc
      781 agcgcattgc agaggagggc caccgtggag cataagccca atttaaaact gtatgtgctg
      841 gtccgacgcg acctgtcgcc gtcacaggtt gccgtccagg cgtgtcacgc cgtggcgaca
      901 tttgtcagcc ggcacgggcg cgacccgtag gtcttttcct gggttgaaga tcaccgcacg
      961 ctggttctgc tcggtgtcaa aaacgaagcg tcgctccaac gctgggaaga aaccgttcgg
     1021 gcttacggga ttgtgtgcga acacttccgc gaggccgatt tagacggcga gatgacagcg
     1081 ctggccatcc accccacggt ggatggtcgg ctctttcgca aactgcggtt gatttgaacg
     1141 agtacaggtt ccagaaaaga ctaaaaggac gaacatggaa gggcgctgtt gctttgaacg
     1201 gcgcccgatt tcaaaaaccc ggaaccctga taagatgaca aagtgacaaa gtgacaaagt
     1261 gacagagtga tttattttca tccctcatcc ctcatccctc atcctttcca aaaccccgga
     1321 attccattga atttttctgg gttccttttg ataattcaga cttcagaata aagcgcgcgt
     1381 cttactgcca ttccaacttc aatattgagc tcagatccct cattttaagc ctagaaaaga
     1441 ttcaggtttt ttattatgcg tcgaattatt ttcagcttgc tttttgccta tctcattgtt
     1501 ttaacaacta tttggatacc aacttattcg cacactgcat tgttatcaaa acgaaatcga
     1561 gcactggtgg ctactgtccg ggcccaatca gccccaacca tttcatcaat ttcaccgact
     1621 caaactgcaa agggcactgc cccaactaaa attgtcatca agggcaccgg gtttcaatca
     1681 ggtgacgtgg tgaaatttaa tgacacggcg gtcagtgcca aaatcaaatc cgccaccaaa
     1741 attgcgatca aaaatgcagc ggcgagtttg tttcaaaccg atgtgactta caaagtgacg
     1801 atcacttcca gtagtgggca aagctcaaat agtctggatt ttatcgttgg cactggaagc
     1861 cagaccaata ccggtgagcc ggcttcgatt gtgattactt cgccgacgtc gtttgtgctg
     1921 aacacaccca atgccctgac gtccaatact gttgct
//
LOCUS       DAOQDO010001314         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58663_length_1952_cov_3.664734, whole genome shotgun sequence.
ACCESSION   DAOQDO010001314 DAOQDO010000000
VERSION     DAOQDO010001314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58663_length_1952_cov_3.664734"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKZ53_18395"
     CDS             complement(<1..803)
                     /locus_tag="PKZ53_18395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ42466.1"
                     /translation="MEAESYIENGSVGQTEPNGFVRFLQRVVDVRPHEISALLMACGY
                     FFCLLSSYYILRPIRDEIGVAGGVNNLPWLFTGTLVATLVANPIFSTLVVKFPVKRFI
                     TFTYLFFIGNLIVFYGLLKVLPHEQTVWVSRAFFVWISVFNLFVVSIFWAFMADVFHS
                     SQGKRLFGFIGGGGTLGAIVGSSITVILAQKIGTMNLFLVSVVLLCGCLACVFWFPTH
                     FTKGETESTEKRETHYSENPIGGGVWAGIIHTSKSPYLLGICAYILLFT"
     gene            complement(938..1936)
                     /locus_tag="PKZ53_18400"
     CDS             complement(938..1936)
                     /locus_tag="PKZ53_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ42467.1"
                     /translation="MILGGTGLIGPYQVQYALDRGHKVTMFNRGKTHPDWFPNVERLI
                     GDRNTGDVKALKGREWDVVIDNPASKPSWVRDTTEVLKNSAKQYVFISSISVYADTSK
                     VGMDETTVTATTDDPDPEKDVMKNYGALKRRCEETAEKAFPGRALIIRPGLIVGPGDY
                     SDRFSYWPLRIDRGGEVMAPGDPTDPVQIIDARDLGEWCIRMVEQGDVGIYNATGPKA
                     QLSIAEMLYGIRAVTTADVKFTWVDAKFLEEKQVQPWSDMPVWVPPIGESAGFATCSI
                     ARALAKGLTFRSLADTAKATLDWYKALPPDRQAKLRSGISPQREAEVLAAWHARKK"
BASE COUNT          540 a          519 c          452 g          441 t
ORIGIN      
        1 atggtgaaga gcaaaatata ggcacagatt ccaagcagat acggtgattt tgatgtgtgg
       61 ataattcctg cccaaacccc gccgccaatt ggattttcag aatagtgcgt ttcacgtttc
      121 tccgttgatt ccgtttcacc tttcgtaaaa tgagttggga accaaaaaac acaggccaga
      181 caaccacata acaggacaac tgagacaaga aacaaattca tggtgccgat tttttgggcc
      241 agaatcactg taatcgaaga gccaacaatg gcgccgaggg tcccccctcc gccaatgaac
      301 ccaaacaacc gctttccctg gcttgaatga aacacatcgg ccataaaggc ccagaaaatc
      361 gacactacaa acagattgaa aacgctaatc cagacaaaaa acgcccggct cacccaaact
      421 gtctgttcat gtggaagtac tttcaacaac ccataaaaca caatcagatt tccaataaag
      481 aacaggtacg taaaggtaat aaaccgcttg actggaaact tgacgacgag ggttgagaaa
      541 atggggttgg ccacgagcgt ggccaccagc gtgccggtaa aaagccaggg aaggttattc
      601 accccacccg caacaccaat ctcatcccga attggacgca aaatgtaata actcgacaac
      661 aggcaaaaaa agtacccaca agccatcagc aaagccgata tttcgtgggg gcggacatcc
      721 accacccgct gcaaaaaacg gacaaacccg ttcggttcgg tttgaccaac cgaaccgttc
      781 tcaatatatg attcggcttc caaggaaaac ctccctttct atcctgaaac acgctctttt
      841 cggaaattat gaattatgaa ttatgaagca gaatccctgg tctgtgactc aatcggatat
      901 catcttcata actcataact cataactcac agcggaatta tttctttcgg gcgtgccagg
      961 cagccaggac ttcggcctcg cgttgcggtg aaatgccgga acggagcttg gcctgtctgt
     1021 cagggggaag tgctttgtac cagtcaagtg tcgctttggc cgtatcagcc agcgaacgga
     1081 acgtcaggcc cttggccagg gcacgggcaa tactgcaggt ggcaaatcca gcggattcgc
     1141 caatcggggg cacccagacg ggcatatccg accagggttg tacctgtttt tcttcaagga
     1201 atttggcgtc gacccaggta aatttgacat cagcggtggt cacggcacga atgccataga
     1261 gcatttccgc aattgaaagc tgggctttcg ggccggttgc gttataaatg cccacatccc
     1321 cctgctcgac cattcgaata caccattcgc ccagatcacg agcatcaatg atttgaaccg
     1381 gatcggttgg gtcgcctggg gccattactt caccaccacg atcaatgcgg agcggccagt
     1441 aagaaaaccg gtcagaatag tcgccagggc ccacaatcaa tccagggcga ataatcagcg
     1501 cgcgccctgg aaaggctttc tcagccgttt cttcgcaccg ccgcttgagc gcaccataat
     1561 ttttcatcac atccttttca ggatctggat cgtcggtggt cgcggtgacg gttgtttcat
     1621 ccataccgac cttgctggta tcggcataca cggaaataga tgaaatgaag acatattgtt
     1681 tggccgaatt tttcagcact tcggtggtgt cacggaccca gcttggtttt gacgccgggt
     1741 tatcaatgac cacgtcccat tcgcgacctt tgagcgcttt gacatcgcca gtgttacgat
     1801 caccaatcaa tcgttcgaca ttcgggaacc aatctggatg tgttttcccc cgattaaaca
     1861 tcgtaacctt gtggccacga tccagggcat attgaacctg atatggacca atcaacccgg
     1921 taccgcctaa aatcaaaatc ttgagtggtt tc
//
LOCUS       DAOQDO010001315         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58847_length_1949_cov_2.962513, whole genome shotgun sequence.
ACCESSION   DAOQDO010001315 DAOQDO010000000
VERSION     DAOQDO010001315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58847_length_1949_cov_2.962513"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKZ53_18405"
     CDS             complement(<1..256)
                     /locus_tag="PKZ53_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42468.1"
                     /translation="MSRKEIQKLQNNAPVHYQNVNAGVDFSRREFFKLAGLGVTGFFL
                     SQVTTPLEVVAQSSPTLINTAKNCIFIHLTGAPSHLDTFDL"
     gene            complement(284..>1949)
                     /locus_tag="PKZ53_18410"
     CDS             complement(284..>1949)
                     /locus_tag="PKZ53_18410"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNJ42469.1"
                     /translation="RRNYIDEEVFGKMERDKVPSAGLSTDEEFIRRVTLDLTGRIPTA
                     DQIRQFVADQSPTKRDRLVDSLIGTPEYVDRWTMWMGDLLKNSARASNVIRYAQGRNA
                     FYSAIKYAIERNMPYDQFVTALIAGSGNNFSADAGYVNYLVGAATPMGPRQDTYDTAA
                     VQASTRFLGIETMDCLLCHNGEGHLNALNVWATNTKREQAWGMAAFFSRMQFRPRIES
                     TEPVIRSFDVAELRGGDYELNTDSGNRTPRAPVDGKSVITPQYLFTGERPGPGENYRV
                     AFARMLTKDRQFARATVNYLWAHFFGLGIVDPPTNFDLARLDPKNPPAEPWTLQPSNP
                     ELLEKLADEFIASGYNLQTTMRSITKSSAYQLSSKFAGEWKEEYTPYFARKLVRRLDS
                     EEMYDAISRATGVFPTFNIQYYTSSIQWAMQLPDTFEPVPVRGRPYTQDAFQARMFLD
                     TFGRGDRDQLPRSNIPSILQSLALMNSPAVTNRIRQSAINGTLASQRADDVKTYVDNI
                     FLTVLGRKPTTAEQDQAVAMFQRDRNQGAQDLMWVLVNKIDFLYNY"
BASE COUNT          435 a          495 c          546 g          473 t
ORIGIN      
        1 tgaggtcaaa ggtgtccaga tggctcggcg caccagttaa atggatgaaa atgcagttct
       61 tcgcagtgtt gatcagggtt ggagatgact gagcgaccac ttccagcggc gtcgtgacct
      121 gactcaggaa aaacccggtg accccaagtc cagccagttt gaaaaactcc cggcggctaa
      181 aatcaacccc ggcgttgacg ttttggtagt ggacaggtgc gttgttttga agtttctgaa
      241 tttctttgcg agacatacga atgttcctcc ctccactgcc acgtcagtag ttgtacaaaa
      301 agtcgatttt gttgacgagc acccacatca aatcctgggc accttgattg cggtcgcgct
      361 gaaacatcgc gactgcctga tcctgttcag ccgtcgttgg tttgcggccc agtacggtca
      421 ggaagatgtt gtccacatag gttttaacat catctgctct ttggctggcc aacgtgccgt
      481 taatcgcgct ctggcgaatg cgattggtga cggcgggact gttcatcagt gccagacttt
      541 gcagaatgga aggtatgttg ctccgaggca actggtctcg atcaccacgg ccaaacgtgt
      601 cgaggaacat tcgggcctgg aaggcatcct gggtgtatgg acgaccacgg acaggcactg
      661 gctcaaaggt gtcgggcaat tgcatcgccc actggatgga actggtgtag tactgaatgt
      721 taaaggttgg gaagacaccc gtggcgcgtg aaatcgcgtc gtacatttct tcagaatcga
      781 ggcgacgaac cagcttgcgg gcgaaatacg gggtgtattc ttctttccat tcgcctgcaa
      841 attttgagga caactgatag gcactcgatt tggtgattga ccgcattgtg gtctggaggt
      901 tatagccact ggcaataaat tcatcggcca gtttttccag cagttctgga ttggagggct
      961 ggagcgtcca gggttcagcc ggcgggttct tcggatcaag tcgggccaga tcaaaattgg
     1021 ttggcgggtc aacaattccc agtccaaaga agtgggccca caggtaattg accgtggcgc
     1081 gggcaaattg ccgatctttg gtcaacatcc gggcaaaggc aacccgataa ttttcacccg
     1141 gtccaggtcg ctcgccagta aacagatatt gtggtgtgat cactgatttt ccatcaaccg
     1201 gtgcgcgggg tgttcgattg ccggaatcag tgttgagttc atagtctccg ccacgcaatt
     1261 cagccacgtc aaaactgcga atgacaggct ccgtgctttc aatccgtggt cggaactgca
     1321 tccgcgagaa gaaagcggcc attccccagg cctgctcgcg tttggtgttg gtcgcccaca
     1381 cgttgagtgc gttgaggtga ccttcaccat tgtggcacag caggcaatcc atggtctcaa
     1441 tccctaaaaa gcgggtgctg gcctgaaccg ccgccgtgtc ataggtatcc tggcgtggtc
     1501 ccatcggggt tgccgctccg accagataat tgacgtaccc ggcatcagcg ctaaagttat
     1561 ttcccgatcc ggcaatgagt gccgtcacaa actggtcata gggcatgttg cgctcaatgg
     1621 catacttgat cgcagagtag aatgcgttgc ggccctgggc atagcgtatg acattggacg
     1681 cacgcgctga atttttcagc aaatcaccca tccacatcgt ccagcggtca acatattcag
     1741 gcgtcccgat caatgaatct accagccggt cacgtttggt tggtgattgg tctgccacaa
     1801 actggcgaat ttgatcagcc gtaggaatgc gaccggtcag atcaagcgtc acccggcgga
     1861 taaattcttc gtcggttgac agtccggcgg atggaacttt atcgcgttcc atcttgccaa
     1921 agacttcttc gtcaatatag ttgcggcgg
//
LOCUS       DAOQDO010001316         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58891_length_1948_cov_2.823032, whole genome shotgun sequence.
ACCESSION   DAOQDO010001316 DAOQDO010000000
VERSION     DAOQDO010001316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58891_length_1948_cov_2.823032"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1294..>1948
                     /locus_tag="PKZ53_18415"
     CDS             1294..>1948
                     /locus_tag="PKZ53_18415"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNJ42470.1"
                     /translation="MKTPRSRSIPFLFTLLMAVSSLSLPLGQIRGTAASGLSPEPVPQ
                     YPTIGLNKVMCPNPDAKALAVSDVNRIIQQAVARALNLGVKATIAVTDREVNVLAVYR
                     MKGANRFGRIEGGTTPGPLELPSVPNIQTEFMAISKAGTGSVFQTTGNAFSPRSASFI
                     IQENIPPGVNFSPGGPLFGVQFSNLFLISDVNPRLPLGISGDPGAVPLFMGEIPVGGI
                     "
BASE COUNT          465 a          466 c          487 g          530 t
ORIGIN      
        1 gacatcacag atatcagaca tgtggcaaag aggaaaacca gcaaacggtg caggccactg
       61 ttcataatcg ccgctccttg taaggttgtc gttgcgcaaa cacaatcaaa ttccagtcat
      121 gagcgatccc tcggtttggc tggaaccggg tttgggcaac gtcagggctc aaataggggg
      181 gtaatgcttt gaagatattt caacgacagt gcttgcgctt ccaaaaaatt cctcactggt
      241 caagaacaaa agggtttcac cccaaggagt cagaatttgg gttgaaatcc tggcacaagt
      301 ggtcgtgact tgttttggat tcaaaaagat caggttagag agcttccagg tgaatggtga
      361 tgcgaaggcg gctttttgaa aggagttaaa gcctgagatt gagagaaccg catcacgcca
      421 gtttggggtg gagcattcaa accggcagtg ggaaaatgtt gattgattgc tgttgtctgg
      481 atgggtagct ggattccgtg cgccaataat agtcttgaat ttttcatgaa gtcaaatttt
      541 ggtctgtatt tttttcaaga ttttggtatt gaatgctaat ttatcttttc taatcaaatc
      601 caatccaaat atagcaatat aaaacaggtc aaatgacgat cacctctcga atcttttgac
      661 ttgccaaggg tggaatacct gaattacagt tctggcgctt catacaggtc atccgtattc
      721 tcttcgcgct gaggttcagg cggtgggtct ggctgatcat cttgattgag actgaatagc
      781 agcagtggcg tgaagtgcct gctatcgagt tggccgtagt ccaaccgccc gccgagaacc
      841 ttcgggtcaa tccggcgaag gtcaatgcaa agaatcgtag ctgatgcatt atttggttat
      901 ggttttagca actattctct gggcgcttgt ccaacagttt tcaataaact cctcattttc
      961 taacgaatcg ttccacacca cgcacgggtg taaacgtgct cgtttactca agatttcagg
     1021 tttttttcat cagtggttgt ttttgattgt ctttcgaggg taatgggtca tacatcgtct
     1081 ggattcccgc tcaacgtgca gacccaatcg tggtgaacct cccctaaccg gggttgttcg
     1141 actgcatcct gaacagtaac tgacttgaag tccagtgcca tccaggtgga gggagggccg
     1201 tcctctcttc aactatctgt ttgccgggtg agtttttgtc tcggcacaca tggcctgggc
     1261 tcaggtacat tttcgtaagg aaggcatgat cccatgaaaa cccccagatc tcggtcaatt
     1321 ccttttttgt tcacgttgct gatggcggta tcatcgttat cgcttcccct tggtcaaatc
     1381 agagggacag cagcttccgg tctgtcgccg gaaccagtcc ctcaataccc aaccattggg
     1441 ttgaacaagg tcatgtgtcc caatccggac gcaaaggcac tggccgtctc tgacgtgaac
     1501 cggataattc aacaggcagt ggcccgagcc ctgaacctgg gcgtgaaagc caccattgcc
     1561 gtcactgatc gcgaagtcaa cgtcctggcg gtataccgca tgaaaggggc caaccggttt
     1621 ggccgaattg aaggcggtac gacccctggt ccgcttgaac tcccctcggt gccaaatatt
     1681 caaactgaat tcatggccat ttccaaagca ggcactggat cagtatttca aacaactggc
     1741 aacgcatttt ctccgcgttc agccagcttt atcattcaag aaaacattcc accgggggta
     1801 aacttttccc caggcggccc gctgtttggg gttcaatttt ccaacctctt tttaatcagc
     1861 gacgttaatc cacgcctgcc attgggcatc tctggtgacc ctggtgccgt gccgctcttt
     1921 atgggtgaga tcccggttgg cgggattg
//
LOCUS       DAOQDO010001317         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58899_length_1948_cov_2.492868, whole genome shotgun sequence.
ACCESSION   DAOQDO010001317 DAOQDO010000000
VERSION     DAOQDO010001317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58899_length_1948_cov_2.492868"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKZ53_18420"
     CDS             <1..367
                     /locus_tag="PKZ53_18420"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNJ42471.1"
                     /translation="TTVREAAESTASDVRRAQEQEATAILAKIPASAYVVLLDVGGEM
                     VSSEKLAEMIERHQNEATKDLYFVIGGFSGVAESVRKRANWRWSLSKLTFTHEIAQVL
                     VAEQLYRAYTILHRHPYSK"
     gene            complement(1003..>1948)
                     /locus_tag="PKZ53_18425"
     CDS             complement(1003..>1948)
                     /locus_tag="PKZ53_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ42472.1"
                     /translation="QPPGIEFRRGHIMDSDAIPEMTEIPRSLLVIGAGVIGVEYATIF
                     ASLNVEVTLVDGRSTILDFVDREVIDMLMYHMRDLGITIRLGEAVASVEWVRNDRIVA
                     TLESGKRIITQSILVATGRRAATDNLNLAVAGLHTDDSGRLKVNDYFQTEVPHIYAAG
                     DVIGFPSLASTSMEQGRNAARHACDREVFRLNEVYPYGIYTIPEISTVGKTEAELTKA
                     KIPYETGVAQYRDIVRGRILGDKAGMLKILFHLETHEILGVHAIGEGATELIHIGQAV
                     MAHGGKLDYFVDSVFNYPTLAEAYKVAALDGISKLSFA"
BASE COUNT          468 a          485 c          511 g          484 t
ORIGIN      
        1 cacaactgtg cgggaggccg ccgaatcaac cgccagtgat gtccgacggg ctcaggaaca
       61 ggaagccacg gcgattctgg caaaaattcc agccagtgcg tatgtcgtgc ttttggatgt
      121 tggcggggaa atggtttcgt ctgaaaaact ggcggaaatg attgaacgcc accagaacga
      181 agccaccaaa gacttgtatt tcgtcattgg tggcttttcc ggtgtggctg aaagtgtcag
      241 aaaacgtgct aactggcgct ggtcgctctc aaaattgacc tttacccatg aaattgccca
      301 ggtgctggtg gctgaacagt tgtatcgagc ctacacaatt ctgcaccggc atccttattc
      361 gaaataaggg gttatcaggg ttcagggttt gggagccgtc aattgaagcg tcttcctcaa
      421 tttgtggttc gactggttca gaaactggtt ttttctcagg tggcgtcggc ggttttagat
      481 agagcttaac cttgacgtta tgcggcagag agacttcgcg gacttggtca tagccgacat
      541 aaagggtttc tttgattgat atttcctgac cattgccagt gcccaggttc aagttcaggt
      601 tgaccatcac aagctggctt ccccgaaaat tccgggcgtg cattgaatag gaataattgt
      661 aaatcaggga ggcattggct gggtcactcc ttctgcccga acaggcgact cctccagccc
      721 ctccaacgcc gacaaatcgt ttcaatggga tttcaattgg tgcgattcca cccaaccctg
      781 gaagaatgaa tttcactatc acagcttcgt atttgggtgg tttggtttgg gcagcgacgc
      841 tttgctggga gcaaaatcca aaagacaaca ggatcaagac aaaaacgacc gggaacttcc
      901 aatttattga tttcattggg tacctcagag aagggattca gttcagcttc gattttgaga
      961 tgtgtgcgat actgaactaa accccacccc ctggcccaaa tctcacgcaa aactgagttt
     1021 gctgattcca tccagtgcgg cgactttgta ggcttcggcc agtgtcgggt agttaaagac
     1081 tgaatccaca aagtagtcga gtttcccgcc gtgagccatc accgcctgac caatgtgaat
     1141 gagttctgtc gcgccttcgc cgatggcgtg aacgcccaga atctcatggg tttccagatg
     1201 aaatagaatt ttgagcatcc cggctttatc gcccaaaatg cgtccccgaa cgatgtcgcg
     1261 atattgcgct acacccgttt cataaggaat tttggctttg gtcagttcag cttcggtttt
     1321 accaacggtt gaaatttccg gaatggtgta aatgccgtaa gggtagactt cgttcagccg
     1381 gaagacttcg cgatcacagg catgccgcgc tgcattgcgg ccctgttcca tactggtgga
     1441 tgccagtgac ggaaagccga ttacatcgcc agcggcataa atatgtggga cttcagtctg
     1501 aaagtagtca ttgactttca agcgccctga atcgtccgtg tggagcccag ccaccgccag
     1561 attcagattg tcagttgctg cccggcgacc ggtggcaacc agaatgcttt gagtaatgat
     1621 gcgtttgcca gattcaagcg tggcaacgat gcggtcattg cgaacccatt cgacgctggc
     1681 cacggcttcg cccagccgaa tggtgattcc caaatcgcgc atgtgataca tcagcatgtc
     1741 aatcacttcg cggtccacaa aatcgagaat ggttgagcgg ccatccacca gcgtgacttc
     1801 gacattgagc gaagcaaaga tggtggcata ttcgacgcca atcaccccag cgccaatcac
     1861 gagcaggctg cgtggaattt cagtcatttc tgggatggca tcagaatcca taatgtgccc
     1921 gcgccggaat tcaattcctg gtggctgg
//
LOCUS       DAOQDO010001318         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58922_length_1948_cov_1.927628, whole genome shotgun sequence.
ACCESSION   DAOQDO010001318 DAOQDO010000000
VERSION     DAOQDO010001318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58922_length_1948_cov_1.927628"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..>1948)
                     /locus_tag="PKZ53_18430"
     CDS             complement(112..>1948)
                     /locus_tag="PKZ53_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42473.1"
                     /translation="SVTPPRMDGIALWLQPMESRALAEFQGRIYVATAGGLVAYEPSG
                     KLVRHYTAADGLPEHDLTSLAVFQNKLYLGTRTQGLLSFDGTVFTQYTIRKPQARSVS
                     ALCSTPRYLLIGTLDAGLFDFDGLVFSQRFQPSKGPEITGVTAIWADQSRLYVGTQKN
                     GLFVWRDGELRVYGTADGLPSPRVTAIVSSGGDILVATDIGVVQLTDTPPFLVLSKQP
                     NLTSLAEFQGKIWGGLVTGGAVEISRTPTPVERASSRFFAGGVSLGGTNPSRTVLAVA
                     DTHLWALTEAGMFQLEPQPNQKASEWKRWGSVPAGKTPLASPHVTSLAVDERGLLWIG
                     YFERGLDVVSLTTGEREHHFQDEAVREINFLTSDPDTGSMLAATSAGLVVYDAGFKPR
                     RYDEKSASLIGNNVAQVLRVPGFKAGSNVAPSSDVPLVLATARGLTVMQSGISRSLTA
                     FHGLASNYLYCAELVGGKLYLGSLGGLMELDGLRVVRTWKTDTSKLSANWINALKAVN
                     GTLYVGTYGGGVCALTPTGDIVPFEETASIEVNPNAMASDGERLYVGTLDQGLLLFDL
                     TRREWRRWRTGLASPNVTSIAIHNGIAYCGTTGGLTQVEVKRLVE"
BASE COUNT          420 a          599 c          516 g          413 t
ORIGIN      
        1 cagggttcag ggttcagggt tcagggttca gggttcaggg ttcagggtct tcgaaggtat
       61 gtccttttgg tccttttggt ccctttcgtc cttttcctgg tcggccccca actattccac
      121 cagtcgcttc acctcgacct gggtcaaacc gccagttgtt ccgcaatagg caattccgtt
      181 gtgaatggca atactggtca cattgggcga agccagccct gtgcgccagc gccgccattc
      241 acgacgggtc aggtcaaaga gcaacaaccc ttgatcaagc gtcccaacgt agagccgttc
      301 cccatcgctg gccatggcat ttgggttgac ttcgatgctg gcggtttctt caaagggaac
      361 gatgtcgccg gttggtgtca gcgcacaaac gccgccgcca tacgttccga catagagcgt
      421 tccattgaca gcctttaaag cgttgatcca gttggcgctc agtttggaag tatctgtttt
      481 ccaggtgcgc acgacccgca gcccgtcaag ttccatcaaa ccgcctaaac tgcccagata
      541 cagttttccg ccaaccagtt cagcacagta gagataattg ctggccaacc cgtgaaacgc
      601 ggtcaaactc cggctgatgc ccgattgcat caccgtcaac ccacgggctg tggccagcac
      661 cagcggcaca tcagaggatg gcgcgacgtt tgagccagcc ttaaagcccg gtacccgcag
      721 cacctgtgcc acattattcc caatcaagct ggcggatttt tcatcatagc ggcgcggctt
      781 gaatccagcg tcatacacta ccaatccggc tgacgtcgcc gccagcatgc ttcccgtgtc
      841 cgggtcagag gtcaaaaaat tgatttcccg gaccgcttca tcctggaaat ggtgttcgcg
      901 ctcgccagtc gtcagcgaca cgacatccag accacgttca aagtatccaa tccagagcaa
      961 tccccgttca tcaacggcca gcgaggttac atgcggcgaa gccagcggcg ttttcccggc
     1021 gggcacgctt ccccagcgtt tccactccga ggctttctgg ttgggttgcg gttcaagctg
     1081 aaacattcca gcttcggtca acgcccagag atgggtatcg gcgacggcca gcacggtacg
     1141 acttgggttg gtgccgccca gcgacacgcc tccggcaaaa aagcgcgaac tggcgcgttc
     1201 aaccggtgtc ggcgtccgcg aaatttcaac cgcgccgccc gtcaccagtc cgccccagat
     1261 ttttccctga aattcagcca gcgaagtcaa attcggctgt tttgaaagca ccagaaatgg
     1321 aggagtatcc gtcagttgca ctacaccaat gtcggttgcg accaggatat cgccaccgct
     1381 tgagacaatc gctgtcaccc tcggcgaagg caatccgtca gccgtgccat acacccggag
     1441 ttcgccatcg cgccagacaa acaggccatt tttttgcgtg ccgacataca ggcgggattg
     1501 atcggcccaa atcgcggtca ctccagtgat ttccggccct tttgatggtt gaaaccgctg
     1561 actaaagacc agtccgtcaa aatcaaagag ccccgcgtcg agcgttccaa tcagcagata
     1621 gcgtggtgtc gaacagaggg cactcaccga gcgagcctgc ggtttgcgaa tggtatattg
     1681 ggtaaacact gttccgtcaa aactcagtaa gccctgcgtc cgcgtgccga gatagagctt
     1741 gttttgaaag accgccagcg acgtcagatc atgttcgggc aggccatcgg ctgcggtgta
     1801 gtgccgcacc agcttccctg acggctcata agcgacgagt ccgccggcag ttgcgacata
     1861 gatccgccct tgaaactcag ccagcgcccg actttccatc ggctgcagcc acagggcaat
     1921 gccatccatc cgtggtggag tcaccgac
//
LOCUS       DAOQDO010001319         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_58988_length_1946_cov_3.951348, whole genome shotgun sequence.
ACCESSION   DAOQDO010001319 DAOQDO010000000
VERSION     DAOQDO010001319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58988_length_1946_cov_3.951348"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKZ53_18435"
     CDS             <1..212
                     /locus_tag="PKZ53_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42474.1"
                     /translation="AEGAAQAAAEMSAALVVRKEGGVRELARRVRDRVTDEKPAPRAE
                     TQPAEPQPQFKAVDTDPPEEAQPAS"
     gene            435..1688
                     /gene="hutI"
                     /locus_tag="PKZ53_18440"
     CDS             435..1688
                     /gene="hutI"
                     /locus_tag="PKZ53_18440"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNJ42475.1"
                     /translation="MTEHLWINLAQLATPIGSRACGAEPMNSLQVIENAAVLVRDGKI
                     AALGVTDEIRAVASEQAEVHDATGLTMLPGLVDAHTHPVFAGTRADEYEQRILGKTYK
                     EISAAGGGIRRSVRLTRAATEDQLTELAVHRARAFLAHGTTTIEGKSGYGLSLDDEFK
                     LLRVLRTMNQTTPLEVVPTFLGAHEIPDEYRDRRDEYLTLLIETMLPAVAKEQLAVNC
                     DIFCEPHVFSLEESRTYLKAATQLGFGLRVHVDQLTRSGGSHLAAEVGAISADHLEQA
                     EEEDLLALKAAQVFPVLLPGSVFHLGLKKYPPARRMIELELPVVLATDFNPGSSPSLS
                     LPMAMSLACTQMRMTPAEALTACTLNAAHSLGLGDRIGSIEVGKQADFVMFDVTDYRQ
                     IPYFFGIHLVQATVKRGEVVWSAAA"
BASE COUNT          423 a          536 c          510 g          477 t
ORIGIN      
        1 aagctgaagg tgccgcccag gcggcggctg aaatgtcggc ggcactggtg gttcgaaaag
       61 aaggtggggt gcgagaactc gcccgccggg tgcgtgatcg ggtcacagac gagaaaccgg
      121 cacccagggc tgagacgcag cccgccgagc cccaaccaca attcaaagcc gttgataccg
      181 atccacctga ggaagcccaa cctgcaagct gaagcattgg gctgagggct gaagaaaacg
      241 ggctgaagac gtcgggctga agacagtggg cggaaaactt cgggttgaag aattggtttt
      301 ctttcatccc tcatcccttg gttccagccc tgagcccgtt ttcttcagcc caatgtcttc
      361 agccctgagc ccgttttctt cagcccaatg tcttcagccc tgagtccctg gcccactgtc
      421 ttccacccaa ccctatgact gaacatcttt ggatcaatct tgcccagctt gccacaccca
      481 tcggttcccg agcctgtggc gctgaaccga tgaactcgct gcaggttatt gaaaatgctg
      541 cggttttagt gcgtgatgga aaaattgctg ctcttggcgt gaccgatgaa attcgggccg
      601 ttgcctcgga acaggccgag gtgcacgatg ccaccgggtt gacgatgctt cctggactgg
      661 ttgatgccca tacgcatccg gtgtttgctg ggacacgagc cgatgaatat gaacagcgga
      721 ttttgggcaa aacctataaa gaaatttcgg ctgctggcgg cggcattcgg cgctcagtgc
      781 ggttgacacg agctgcaacc gaagatcaat tgacagagct ggcagttcat cgcgcccgcg
      841 cttttctcgc gcatggcaca accactatcg aaggcaaaag tggatatggg ctttcattgg
      901 acgatgagtt caagctgctg cgagtcctgc ggacaatgaa tcaaaccacg ccgctagaag
      961 tcgtccccac atttcttggc gcccacgaaa tcccagatga ataccgtgac cggcgtgatg
     1021 aatacctgac cctcctgatt gaaaccatgc ttccagccgt tgccaaagaa caactggcgg
     1081 tcaactgcga tattttttgc gagccacacg tgttttcact tgaggaatcc cgcacctacc
     1141 tgaaggctgc cacacagctt ggatttgggc tgcgggtcca tgttgatcaa ttgacccgct
     1201 caggtggttc acatctggct gccgaagtcg gcgccatttc agccgaccat cttgaacagg
     1261 ccgaagaaga agaccttctg gcgctcaaag cagctcaggt ttttccagta ttgctgccag
     1321 gctcagtttt tcaccttgga ttgaaaaaat atccgccagc ccgccgcatg attgaactgg
     1381 aactcccggt tgtcctggca accgacttta atccagggag ttcgccttcg cttagcctgc
     1441 ccatggcgat gagtctggcc tgtacccaga tgcgaatgac acccgccgaa gccctcactg
     1501 cctgcaccct caatgcggcc cacagtctgg ggctcggaga ccggattggt tcgattgaag
     1561 tcggaaaaca ggctgatttt gtgatgtttg atgtcaccga ttaccgacaa atcccttact
     1621 tttttgggat tcatctggtg caggcaaccg tcaaacgcgg tgaagtcgtg tggagtgcgg
     1681 cggcttgacg ccgctttcct atacaaagcg gcgtcaagcc gccgcactcc acattgtttt
     1741 tttcctgcct tcatttcaac atttctgata ttctccccga ttctattttc ccactatcgc
     1801 ttactcgttc taacccgtcg caaatgaatt ggttcaaggt gcttttcaag ggcacctgaa
     1861 ttttggctgc attgtcagtc gaggaggatt tctaccttca tgaaagtttt gtgttctgtg
     1921 tggtttacag cgattttttt gtttag
//
LOCUS       DAOQDO010001320         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59000_length_1946_cov_3.103120, whole genome shotgun sequence.
ACCESSION   DAOQDO010001320 DAOQDO010000000
VERSION     DAOQDO010001320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59000_length_1946_cov_3.103120"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="PKZ53_18445"
     CDS             complement(<1..1734)
                     /locus_tag="PKZ53_18445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ42476.1"
                     /translation="MNEQEFAQQVQALEQYALEHPEAYKIRAALLACLGYGFLLVLLG
                     LIGALLIGLIALMSSGGSVVFVKLLIPILLFAAVILQAIFRALWIKIPAPQGYEIHPD
                     QAPELFQFLRETRTKLRSPRLHHVLITDDFNAAIVQVPRLGALGWSANYLVIGLPLMQ
                     ALTTEQFESVLAHEMGHLSRSHGWVSVWILRFRYTWATLMEQLEAQETVGSSIIESFI
                     QWYAPYFNAYTFVLARMREYEADQAAATLVGVRATAEALIETEIKSQYLEQSYWRNIL
                     RKANQQASPPTGIYAEIPMAFRQPLPSDLITSTLKNTLAIKTGYDDTHPALNDRLVAL
                     GFTPPKLSDQTHWSDHYQLSSLKESAAQVLLGAAYQLTVSHFEDEWKQKIGSDWEERY
                     QYVQKSSQILAEFDARREQNLEFDESDLWKYAALTAEFQETEKALPLLTQLLKENPQH
                     TEANFLYGQCLLKNGDAAGIPYIERAIEQDFQNSEPGYQILLQFCLDHELKDEFEKHK
                     LQLIRFYDRMEMARRERQSVTEHQLKSHRMSPEVVRRLSDQLAAFPQVKIAYLAEKDV
                     RVSPEIPYLVLG"
BASE COUNT          497 a          495 c          474 g          480 t
ORIGIN      
        1 cccaagcacc agataaggaa tttcagggga aacccgaaca tctttttcag ccagataggc
       61 aattttgacc tggggaaaag ctgccagttg atcgctcagg cgtcgaacaa cttctggtga
      121 catgcggtgc gatttcaatt gatgttcggt gacagattga cgctctctgc gagccatttc
      181 catccgatca tagaatcgaa tcaattgcag tttgtgtttt tcaaattcat ccttcaactc
      241 gtgatcaagg caaaactgga gcaagatttg ataccctggc tcagaatttt gaaaatcctg
      301 ctcaatcgct cgctcaatat atggtattcc agcggcatct ccatttttaa gcaggcactg
      361 accatagaga aaatttgcct cagtgtgctg tgggttctct ttgagcaatt gtgtcagcaa
      421 cggaagcgct ttctccgttt cctggaattc ggcggtcaat gccgcatatt tccataaatc
      481 tgactcatca aactcaagat tctgttccct gcgggcatca aattcggcca ggatttggct
      541 ggatttctgc acatactggt agcgttcttc ccaatctgac ccaatctttt gtttccattc
      601 atcctcaaaa tggctcaccg tcagttgata ggccgccccg agcaggacct gcgccgcgct
      661 ttccttgagc gatgaaagct ggtaatggtc actccaatgt gtctggtcag atagctttgg
      721 cggagtaaac ccaagtgcaa ccaaccggtc gttgagcgca ggatgggtgt catcataccc
      781 ggttttgatc gccagtgtat ttttcagcgt ggaggtgatc aggtctgaag gcaatggctg
      841 gcgaaaggcc atagggatct cagcataaat ccccgtgggt ggtgaagctt gctggtttgc
      901 tttccgcaaa atattgcgcc agtacgattg ttccagatat tgagacttga tctcagtttc
      961 gattaaagct tcagccgtgg cccggacacc aaccagcgtg gcggcggcct gatcagcttc
     1021 gtactcgcgc atccgggcca acacaaaggt atacgcgttg aaatacggag cgtaccattg
     1081 aatgaacgat tcaatgattg aactaccgac cgtttcctgt gcttcaagct gttccatcaa
     1141 tgtggcccag gtataccgga accggagaat ccagacgctc acccagccgt gggaacggga
     1201 caaatggccc atttcatgcg ccagaaccga ttcaaattgc tctgtggtca gagcctgcat
     1261 cagcgggagg ccaatcacca gatagttggc cgaccaccca agggcgccaa gtctgggaac
     1321 ctgcacaatc gcggcattaa aatcatcagt gatcagcacg tgatggagcc ggggtgaacg
     1381 aagtttggtt ctggtctctc gcaaaaactg aaacagttcc ggtgcctgat ctgggtggat
     1441 ttcatacccc tgtggcgccg ggattttgat ccatagcgcg cgaaaaatcg cctggaggat
     1501 cacggcggca aacagcagga tcggaatcaa caatttgaca aacaccacac tcccaccgga
     1561 actcatcaaa gcaatcagac caatgagcag ggcgccaatt aaaccgagga gcaccaggag
     1621 aaacccgtac cccaaacagg caagtaaggc agcccggatt ttataagctt ccgggtgttc
     1681 caacgcatat tgttcgagtg cctgaacttg ttgggcaaat tcttgttcat tcataaattg
     1741 tgccgccacg tgtttgattt caatcaggta aaacagatag caccggatga ctggtggttc
     1801 gcttcacaaa aaagctggtt tcttcacaat caggcaacct gattgcatgt tcactcaagc
     1861 attactttca catcacgctg gtggtggcca gcgatacagg atcccacaat tcaatttgga
     1921 gatctggcaa ttatcctcac acaccg
//
LOCUS       DAOQDO010001321         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59003_length_1946_cov_2.989952, whole genome shotgun sequence.
ACCESSION   DAOQDO010001321 DAOQDO010000000
VERSION     DAOQDO010001321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59003_length_1946_cov_2.989952"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKZ53_18450"
     CDS             complement(<1..930)
                     /locus_tag="PKZ53_18450"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNJ42477.1"
                     /translation="MANEDYYALLGVTKSASAEEIKKAYRKLARKYHPDLNPGDKVAE
                     EKFKKISEAFEVLSDPKKREIYDKFGSYNESYANAGPGGFGGFDFGDMGSGSFRDIFS
                     ELFSGGRAAASAGPSTQRGADIEYPLAVSFEDAANGLTATITISRNEGCTNCNSTGDG
                     PGPTTTCGTCNGSGQRGPNRMGFGLNMPCSACNGTGKRRPICVACRGQGVLLKSEAVK
                     VRIPAGVDTGSRVRVTGKGHAGVRGGQAGDLLIITNVGVHQYFVRKGDNIHCTIPITV
                     PEAALGTKIEVPTINGKAVLRIPPGTQSGQQFRL"
     gene            complement(1095..>1946)
                     /locus_tag="PKZ53_18455"
     CDS             complement(1095..>1946)
                     /locus_tag="PKZ53_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNJ42478.1"
                     /translation="NPDEVVAVGAAVQAGVLAGDVKDLLLLDVTPLSLGIETLGGVTT
                     VLIPRNTTIPTRKSEVFSTAADNQTSVEVHVLQGERPMARDNRTLAQFRLIGIPPAPR
                     GVPQIEVTFDIDANGIVNVSAKDLGTGREQKITITASSGLAKDEIDRMVRDAESNAAT
                     DQQRREQIESKNRLDSLVYQTEKALSENRDKVDAATISEIEAALSESKSKLETASLSE
                     LNSLYEKLSRSSQKLAEVIYRQGGADVPPGGSATGASAGAGAGGSTTRSDDNVIDAEY
                     VDVDDKK"
BASE COUNT          429 a          543 c          485 g          489 t
ORIGIN      
        1 caaccggaac tgctgcccag attgtgtacc gggtggaatt ctgagaacag ctttgccatt
       61 gatcgtcgga acctcgattt tggtgccaag cgcggcttcc ggaacggtaa tcggaatggt
      121 gcagtggatg ttatcccctt tgcgcacaaa atattgatgc accccaacat tggtaatgat
      181 caacaaatca ccggcctggc ctccgcgcac accggcatgg cctttgccag ttacccggac
      241 gcgtgaaccg gtgtccactc cggctggaat acgcactttg acggcttcag actttaaaag
      301 tacgccctgt ccacggcagg ccacacagat tggccgccgt ttccccgttc cattacaggc
      361 gctgcagggc atattcaacc caaatcccat gcgattgggt ccgcgctgac cacttccgtt
      421 acaggtaccg caggtggtgg tgggccccgg tccatctccg gttgaattgc agttggtgca
      481 gccttcattg cggctgatgg taatggttgc ggttaaccca ttggcagcgt cttcaaatga
      541 aacggccagc gggtattcga tgtcggcgcc acgctgggta ctgggaccag ccgaggccgc
      601 cgctcgccct ccgctaaata attctgaaaa aatgtcgcgg aatgaaccac ttcccatatc
      661 cccaaaatca aacccgccaa atccgccagg gccggcattg gcataacttt cattataact
      721 gccaaattta tcgtagattt cgcgcttctt tggatcgctg agtacctcaa aggcttcaga
      781 gatttttttg aatttctctt cggccacttt atcaccgggg ttgagatcgg ggtgatattt
      841 tcgggctaac ttgcgatatg cctttttgat ttcctcggct gaggccgatt tcgtgacccc
      901 aagcaaggcg taataatctt catttgccat aaggcagcct caacacaggt tgaatgggta
      961 caatttcagg cgaaagaacc tgccaaaaat caggtccaga aacaggttga ggagaaagcc
     1021 gtttttttct gacagcaaag ccttctcctc ctgtttccgc tacctggtga ttcggttccg
     1081 atgtgtgaaa tggactattt cttgtcgtcc acgtcaacat attcggcatc aatcacattg
     1141 tcatctgatc gggtcgttga accacctgcc ccagccccag ccgatgcacc agtggctgac
     1201 ccgcctgggg gaacatcagc tccaccctga cgataaatca cttcagccag tttttgtgaa
     1261 ctgcggctga gcttttcata caggctgttc aactcagaca gactggctgt ttcgagcttg
     1321 gattttgatt cgctgagtgc ggcttcaatc tcgctgatgg tggcggcatc aaccttatcc
     1381 cgattttcag acaacgcttt ttcagtctga tacacaagtg aatcaagtcg gtttttcgat
     1441 tcgatttgct cacgacgctg ctggtcagtc gccgcatttg actcagcatc gcgaaccatc
     1501 cggtcaattt catctttggc cagtccggaa ctcgcggtaa tggtgatttt ctgctcacgg
     1561 ccagtgccta aatccttggc cgaaacgttc acaattccgt tggcatcaat gtcaaacgta
     1621 acttcgattt ggggaacgcc acgtggggct ggcggaatac caatcaatcg gaactgggcc
     1681 agggtccggt tgtcacgtgc cattgggcgt tcgccctgca gtacgtggac ttcaaccgaa
     1741 gtctggttat cagccgccgt tgaaaagact tcgctcttgc gagtcggaat cgtggtattg
     1801 cgcggaatca acactgtcgt gacaccgccc agggtttcga tccccagtga caatggggtc
     1861 acatccagga gcagcaggtc tttgacatcg ccagccaaaa caccagcctg aactgccgcg
     1921 ccaaccgcca ccacttcatc cgggtt
//
LOCUS       DAOQDO010001322         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59067_length_1945_cov_2.933333, whole genome shotgun sequence.
ACCESSION   DAOQDO010001322 DAOQDO010000000
VERSION     DAOQDO010001322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59067_length_1945_cov_2.933333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="PKZ53_18460"
     CDS             <1..1489
                     /locus_tag="PKZ53_18460"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HNJ42479.1"
                     /translation="DRQSVEFPSPHSPLTELLARLQDKEPAQKLLAAAGTVAWYRQAG
                     QLPVVEADSALTPCELDDLPECSRRSGEQLLSLLGGQHSEVFPEWLSLLAAGGKRVPT
                     RHLPALLEYGRQRRELRDAILPVLGKRGRWLAAHNSNWSYVLDRSETQLDDSIWETGT
                     REAREAVLRRTRATDPAKARELVVSSWAQDPAELRALWLAIFETGLSLDDESLLESAL
                     DDRRKEVRVKAAELLQRLPGSQLQQRMLDRVRPLLSIQRGEKLKLQVTLPEECSKAMV
                     RDGIELKPPHSGIGEKAWWFLQLLKSVPPTFWYQTLAVSAEVLLVLAKKSDWEDVLIE
                     GWLVAAARHQDVLWINTFFHQAGKFPQKWNYLLTDFLRTLPPVFRERYIAEQLKSGRE
                     ILQGSGFVFDVLKSCTHPWSSELSTSFVQCLRLTITSKPNHRDWLLAYELKSLAVRLS
                     PTCLAEAVQGWPTDRTELGGWSDAVEQFLAVTQFRLEMRQAMDLL"
     gene            1532..1831
                     /locus_tag="PKZ53_18465"
     CDS             1532..1831
                     /locus_tag="PKZ53_18465"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HNJ42480.1"
                     /translation="MEITVEAIYEAGVLKLLHPLPQLREHEKVQLVIQTTAQNERDRA
                     AAVLREAGLLAEPSPGTKERAARSAASLAEVQQAFSRSPGKLLSEIVIEQRGPKE"
BASE COUNT          467 a          472 c          526 g          480 t
ORIGIN      
        1 ggatcgtcaa tccgttgaat ttccgtcgcc acattcacca ctcacagaat tactggcgag
       61 gcttcaagat aaagagcctg cccagaaact gctggcagcg gctggcacgg tggcctggta
      121 tcggcaagca ggtcaacttc cagtcgttga agctgactcc gcactgacgc cttgcgagtt
      181 ggatgacctc cctgagtgca gtcggcgttc tggcgaacag ttgctttcac tgcttggcgg
      241 acaacacagt gaagtgttcc ctgaatggct ctctctcctg gcggctggtg gaaaacgtgt
      301 gcctacccgt catcttccgg cattgcttga gtatggtcgc cagcgtcggg aattgcgcga
      361 tgccatattg ccagttttag ggaagcgcgg aaggtggctg gctgcccata actcaaactg
      421 gagttatgtt ttagatcgaa gtgaaaccca actcgacgat tcaatttggg aaactggaac
      481 ccgcgaagct cgtgaggctg tgctccggcg aacacgcgcc acagacccgg ccaaagcccg
      541 agaactggta gtatcaagct gggctcaaga tccagccgaa cttcgggctc tctggctggc
      601 aatctttgaa accgggttga gtctggatga tgaatcgttg ttggaatcag cattggatga
      661 ccgtcgcaaa gaggtgcgtg tcaaagctgc tgaactgctg cagcgacttc caggttcaca
      721 attgcaacag cgcatgctgg atcgggtacg accactgctt tccatccagc ggggagagaa
      781 actcaaactt caggtgacgt tacctgaaga atgcagcaag gcgatggttc gagacggcat
      841 tgaactcaaa ccaccgcatt ccggaattgg tgaaaaagcc tggtggttcc tgcaactgct
      901 caaaagtgtg ccaccgacgt tctggtacca aacgctggct gtttcagcgg aagttttgct
      961 tgtactggcc aaaaaatcag actgggaaga tgttctgatt gaaggctggc tggttgctgc
     1021 cgcccggcat caggatgttc tatggatcaa cacttttttt catcaggcag ggaaattccc
     1081 ccaaaaatgg aattatcttc tgactgattt tctgaggacg ttacctccgg tttttcgtga
     1141 acggtacatc gccgaacaac tcaaatccgg acgggaaatc ttacagggtt cagggtttgt
     1201 atttgatgtt ctcaaaagtt gtacccatcc ctggagttct gaactttcaa caagttttgt
     1261 ccaatgtttg cggttaacca ttacttcaaa acccaatcat cgggactggc tattggcgta
     1321 cgaactcaaa tcactggcag tacgcctgtc accgacctgt ctggccgaag ctgtgcaagg
     1381 ttggccgact gaccgaactg aactgggcgg gtggagcgat gctgtcgaac aatttctagc
     1441 cgtcacccaa tttcgcctgg aaatgcgtca ggcaatggac ttattgtgag ttgtggttgt
     1501 atttcaccat gacctggctc aggaggtatt tatggaaatc acagttgaag ccatttatga
     1561 ggctggtgtt ttgaaactct tgcatccatt acctcagctc agagaacacg aaaaagttca
     1621 gcttgtgatt caaacgactg cgcaaaatga acgtgatcgt gcagccgcgg ttcttcgtga
     1681 agcaggatta ttggctgagc ccagccctgg gacgaaagaa cgggcagccc gttcagccgc
     1741 atcattggct gaagttcagc aggcattttc caggtcgcct ggtaaactgt taagtgagat
     1801 tgtgattgag cagcggggac caaaagaatg agtcttccaa ccctcagccc ttaaggagcg
     1861 ttaaaaatta acttcccgtt ttcattggga ggcaggcatt tggattgagc atcctgaaag
     1921 gctgccgttc ggatagccgg tggtt
//
LOCUS       DAOQDO010001323         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59430_length_1939_cov_2.747877, whole genome shotgun sequence.
ACCESSION   DAOQDO010001323 DAOQDO010000000
VERSION     DAOQDO010001323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59430_length_1939_cov_2.747877"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="PKZ53_18470"
     CDS             complement(<1..1852)
                     /locus_tag="PKZ53_18470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42481.1"
                     /translation="MFQAVHLGTQRPVAVKVIAPDFMANTEFVERFKREAVAAGRLRH
                     PNVVNVTDFGFSLVGSTTLAFLVMEFLDGCNLGELLAQKGQLPLNLVVDIVEQTALAI
                     DEAHKQGIVHRDLKPDNIWLEPNRRGGYNIKVLDFGIAKLRDTCSEGSDTDVSLTPSG
                     RLQPVDTEGSTLINRSTLPFSHQRPSTPVFENTEGATQIHPSNPQVSPSPVVVSVAVD
                     SESGTQIHPTTHSTHFDEPEIPRTDGGRATNLLNHGTELSLVTQVGTVMGTPAYMSPE
                     QCKGVATDGKSDIYSLGVIVYQMLAGEQPFKGDTFQLIAKHTEEPPPSLYEKRIEISK
                     DVEAIVFSALAKDPQERPVNARAFAAALRSTIEGETGVLKTARFMFSTCQRHFFALGF
                     IAYLPTIMLAGALIYLEYASGALNSYYGPLAFIGMLVGLYGFALLGNRYNVAAATIIV
                     EKLVTNPESELSFKPIALTYCRNFWPLTKTIFLSCGSVLGRSLPLVVPGIKAAMQQAL
                     APAAVIVEGKIGKDAIERSTDLMKFHRPVGYAFLVRDISAATFGVLYPHLSMVGFLIY
                     GGDDILGDLIQKIFSVWVGLAIAGLFISTMFHSVNCAIPTALFYFQALRSA"
BASE COUNT          470 a          493 c          529 g          447 t
ORIGIN      
        1 cggctgaccg cagggcctgg aaataaaaca gggcggtcgg aatcgcgcaa ttcaccgaat
       61 gaaacatggt cgaaataaat aaaccggcaa ttgccagtcc aacccacaca ctgaaaattt
      121 tctggatcaa atcccccaga atatcgtccc cgccataaat caaaaaaccg accatggaga
      181 ggtgtggata gaggacgccg aacgttgcgg cgcttatgtc acgaaccaaa aaagcgtatc
      241 caactggccg atggaatttc atcaggtcag tggatcgctc gatagcgtct tttccgattt
      301 ttccttccac aatgaccgct gccggtgcca gggcctgctg catggcggct ttaatacctg
      361 gaaccaccag aggaagagag cgaccaagta ccgaaccaca gctcaagaaa atggttttgg
      421 ttaatggcca gaagttccgg caataggtga gggcgattgg cttaaacgac agttctgatt
      481 cagggttggt gaccagtttc tccacaatga tcgttgcggc ggccacgttg tatcggtttc
      541 caaggagggc gaatccataa agtccaacca gcatcccgat aaaggccagc ggtccatagt
      601 aggaattcaa ggcccctgag gcatactcca ggtaaatcag agcccccgcc agcatgattg
      661 ttggcaggta ggcaataaac cctagcgcaa agaaatgtcg ctggcaggtt gaaaacataa
      721 agcgggcggt tttcaagact ccggtttcgc cttcaatggt cgagcggaga gcggctgcaa
      781 atgcccgggc gtttacgggc cgttcctgtg ggtctttggc gagtgccgaa aacacgatgg
      841 cttcaacgtc tttcgaaatt tcaattcgtt tttcatacag cgacggcggc ggttcttccg
      901 tatgtttggc aataagctgg aacgtgtcgc ctttgaacgg ctgttcacca gccagcattt
      961 gatagacaat cacgccgagg ctgtaaatat ctgatttgcc atcggttgca acccctttgc
     1021 actgttcggg cgacatatag gctggcgttc ccatcaccgt cccaacctga gtgaccaggc
     1081 ttaattcagt gccatggttg agcagatttg tcgcccgtcc accatccgtg cgcgggattt
     1141 ctggttcatc aaaatgagtt gaatgcgtcg tcgggtgaat ttgagtccca gattctgagt
     1201 caacggccac ggataccacc actggtgatg gcgaaacctg cggatttgaa gggtggatct
     1261 gggtggcgcc ttcggtgttt tcaaaaactg gggtactggg tcgttgatgg ctaaagggga
     1321 gcgtactgcg attgatcaag gttgagcctt cagtatcaac tggttggagc cggcccgaag
     1381 gagtcaggct cacgtcagta tcagatcctt cgctgcaggt atcacgcagc ttggcaattc
     1441 caaaatcaag gaccttgatg ttatagccgc cgcgccggtt gggttctagc cagatgttgt
     1501 ccggttttaa atcccggtga acaatgcctt gtttgtgggc ttcgtcaatg gccagagctg
     1561 tttgttcgac aatatcaacc accaggttga gtgggagctg tcctttttgg gccagcagtt
     1621 caccaaggtt gcatccatcc agaaactcca tgaccagaaa ggccagcgtg gttgacccaa
     1681 ccagcgaaaa tccaaaatcg gtgacattga ccacgttggg gtggcgcaac cggccagcgg
     1741 cgacggcttc acgcttaaat cgctcgacaa actcagtgtt ggccataaag tctggggcaa
     1801 tcactttgac cgccactgga cgctgggtgc cgaggtgaac cgcttgaaac acagcgccca
     1861 ttcctccctg gccaagttgt ttgacgatct gatatttccc gtcaagcggt tgcccgagca
     1921 ggctatcgag tgtggacat
//
LOCUS       DAOQDO010001324         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59438_length_1939_cov_2.625796, whole genome shotgun sequence.
ACCESSION   DAOQDO010001324 DAOQDO010000000
VERSION     DAOQDO010001324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59438_length_1939_cov_2.625796"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1939
                     /locus_tag="PKZ53_18475"
     CDS             <1..>1939
                     /locus_tag="PKZ53_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNJ42482.1"
                     /translation="QAESRVQPTYLLPLSARTPEALVSLAERFQTYLASISSQQLFDV
                     CRTASLRRDHYEYRLAVVGATCEELIEKLGVFLAGEISNGLAVGRKIPGQSPKVAFVF
                     SGQGNQWAGMGADLIEYSPPFRKVLKQCDEYLQGYGGWSVFEELQAHAAHSRLNRTDI
                     AQPAIFSLQVALAAFWKQLGIVPDAVVGHSMGEVAAAQVAGVLTLQDALHVIFHRGRV
                     MEKAAGKGKTAAAALSETEFQELLKLFPTLSVAAENSPTSLTVSGDPKSIEAFVEYLH
                     SKGIFSRVITQHYAFHSPQLEFLQHELAGALFGLTPQKAITPIFSTVTGKPSVFGDFS
                     PAYWGRNLREPVCFAQAIEAMASEKYDVFLEMAPHAVLSPAIRECFQSQKLDTAILSS
                     LRRGEHGYVSMCTSLATLYAKGCTINWSGLYATAGQRQKLPGYPWQRERCWSELQINQ
                     GSESGTLAVTPPPDTSALAGWLHQLVWERIQLPHPVTVVPSGHWLIFADTSGIGTALA
                     QSLTATGATCVVVSPGVDFLPQGPHQVQLNPANQAHFKSLFVWLDQTGQTPHRIAYLW
                     PLQAPTTEQFADSTFETTIDTTCTGLLFPLQEILTRQFPQPPVFWIATQGAQALPQDP
                     GLERLTQSPVWGFGRSIARELP"
BASE COUNT          431 a          560 c          502 g          446 t
ORIGIN      
        1 caggctgaat cacgcgtcca acccacctac ctgctgccgc tttctgcccg cacccctgag
       61 gcgcttgtca gcctggcaga acggtttcag acctatctgg catctatttc cagccagcaa
      121 ctctttgatg tctgtcgcac agcgagtctg aggcgggacc actatgagta ccgtctggct
      181 gtggtcgggg ctacgtgcga agaactgatt gaaaaacttg gcgtgttcct ggctggggag
      241 atcagcaatg ggctggcggt tggacgcaaa atccccggcc aatcccccaa agtcgcattt
      301 gtgttttctg gtcagggaaa tcaatgggcg gggatggggg cagatttaat tgagtacagc
      361 cctccattcc gcaaagtact taagcaatgt gacgaatacc tgcaagggta cggtggctgg
      421 tcagtgtttg aggaactcca ggcccatgcc gcccattcca ggctcaatcg gaccgacatt
      481 gcccaaccag cgatctttag tcttcaggtt gcgctggccg cattctggaa acaattagga
      541 attgtcccag atgccgttgt cggacacagc atgggtgagg tcgctgcggc tcaggtggct
      601 ggggtactga ccctccaaga tgccctccat gttatcttcc atcgtggtcg ggtcatggaa
      661 aaagccgccg gaaaaggaaa aacggcggca gcagctcttt cagaaacaga atttcaggaa
      721 ctgctcaaac tgtttccaac gctgtccgtt gcggctgaaa atagcccaac ctcgctcaca
      781 gtgtccggag atccaaaatc aattgaggcg tttgttgaat atctgcactc aaagggaatc
      841 tttagccggg tcattaccca acactatgcc ttccacagtc cccaactcga atttctccag
      901 cacgaactgg ccggggcact ctttggattg acaccacaaa aagccattac tccaatcttt
      961 tcaaccgtta caggaaaacc ctctgttttt ggcgatttct cacctgccta ctggggccgg
     1021 aatttacgcg agccggtttg ttttgcccag gcaatcgaag ccatggcatc agaaaagtat
     1081 gatgtctttc ttgaaatggc ccctcacgcc gtgttgagcc cggcgattcg ggagtgtttt
     1141 caatctcaaa aactggatac ggccatcctc tcatcactga gacgagggga acacggatat
     1201 gtgagtatgt gcacgagcct ggccaccctc tatgccaaag ggtgtacaat caactggtct
     1261 gggttgtatg ccacggctgg tcagcgccaa aagttgcccg gctatccctg gcaacgcgag
     1321 cgctgctgga gcgaactgca aatcaaccag gggtcagaat cagggacgct ggcagtcaca
     1381 ccgccaccag atacctcggc tttggctggc tggcttcatc agcttgtctg ggaacgaatt
     1441 cagttacctc acccggtgac ggttgtacca tctgggcact ggctgatttt tgctgatacc
     1501 tccggcatcg gcaccgcgtt ggcccaatct ctgaccgcca cgggggccac ctgtgttgtg
     1561 gtttcgccag gagttgattt tctgccacag gggccccatc aggttcaact caatccagcc
     1621 aatcaagccc acttcaaatc tttgtttgtg tggcttgacc aaaccgggca gacaccacac
     1681 cggatcgctt atttatggcc acttcaggca cccacgacgg aacaatttgc ggactcaacc
     1741 tttgagacca cgattgacac cacctgcacg ggcctgctgt tccccctcca ggaaatactc
     1801 acccggcaat ttccccaacc gccagtgttc tggattgcaa ctcagggtgc tcaggcactg
     1861 ccccaggacc ccggattaga gcgattgacc caatcgccgg tgtggggatt tggccgttcc
     1921 atcgcccgtg aacttcccc
//
LOCUS       DAOQDO010001325         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59488_length_1938_cov_3.211365, whole genome shotgun sequence.
ACCESSION   DAOQDO010001325 DAOQDO010000000
VERSION     DAOQDO010001325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59488_length_1938_cov_3.211365"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="PKZ53_18480"
     CDS             complement(<1..1910)
                     /locus_tag="PKZ53_18480"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNJ42483.1"
                     /translation="MPGIRVQQLGGPGSQSGIFIRGLRSYDTAVVVDGLRLRDAASTQ
                     GDSLAQIQELFVVDTDRVEVVRGPESALYGTTAIGGVVNVVTAHGGGPVRGQIQLEGG
                     SLGLFRGRAQVAGGAASDRFLYSAGLMHLNVSDGVDGDTPNRNWSGQGFVQYAFRPNI
                     TLSGRILGNTLFAQLTDGSFDAPGASLPPAGSIVKAVALPLDQQRLFEAGKPFTLGNA
                     NFISNLRDPDFRRDSSFLSSAVIFQHQINEKIGYRVNYQRVDTNRKFTDGPAGVRFEP
                     DFKVVNRFDGVIDTLGVQGDWQLNRFFTFSAGYEFEREQYLGRDSDENPIVSARTNVG
                     IDIEQRSHTFFLRNQFRAFDNRFQMSAAFRLQAFDLSNPTFLGGSSPYPISKFPAPET
                     AYTGDGSISYFFRSTNTKLRSHVGSGYRAPSLFERFGSSYFGGFFSGFGDPLLRPERS
                     IGVDAGIDQTFANNRVRVSATYFYTRLQEIVDFDFSGYINPGTDPYGRFFGYLNTGGG
                     LSRGLELSLNTRPIDTLTVTASYTYANADNRRPTSVAGLLRTFVVPEHTFTLTANQQI
                     GRRVEVNFDLLATSNHLFPFSGRAFEFKGPIKGDIVVSYTHPIGEQSLKVYGKVENMF
                     DRTYFESGYRTPG"
BASE COUNT          488 a          502 c          517 g          431 t
ORIGIN      
        1 cctggtgttc gatagccgct ttcaaagtag gttcgatcaa acatgttttc aaccttgcca
       61 tacactttga ggctttgttc accaattggg tgggtgtaac tcacaacgat atcgcccttg
      121 atcggacctt tgaattcaaa cgcccgacct gaaaaaggga agagatggtt gctggttgcc
      181 agcagatcga aattcacttc gacccggcgc ccaatttgtt gattggcggt caatgtgaat
      241 gtgtgttctg ggaccacgaa ggtgcgcaac aatccggcca ccgatgtagg ccgacgattg
      301 tcagcattgg cataagtgta agacgcggtg acagtcagcg tgtcaatcgg gcgggtattc
      361 aaactaagtt ccagtccgcg tgacaaaccg ccaccagtgt tcaggtagcc aaaaaatcgt
      421 ccgtatggat cagttccagg gttgatatat ccgctgaaat caaaatccac gatttcctgg
      481 agccgggtgt aaaagtaggt tgcgctcacc cgaactcgat tgttcgcaaa ggtttgatca
      541 attccggcat ccacgccgat tgatcgctct ggtcgcaaga gtggatcacc aaagcctgag
      601 aaaaatcctc cgaagtatga cgagccaaat cgctcaaaca gcgatggtgc ccgatagccg
      661 ctgccgacgt gagagcggag tttggtattg gtcgaacgga aaaaataaga aattgaaccg
      721 tctccggtat aagctgtttc cggcgctggg aactttgaaa tcgggtacgg tgagcttccg
      781 ccaagaaatg tcgggtttga caggtcaaaa gcctgcaatc ggaaggcagc agacatctga
      841 aatcggttgt caaatgcccg gaactggttg cggagaaaga acgtgtggct gcgttgttcg
      901 atatcaattc caacattggt ccgggcactc acgatggggt tttcatccga atcgcggccc
      961 aggtactgtt cgcgttcaaa ttcatatccg gcagagaagg taaagaaccg attgagctgc
     1021 cagtctccct gaacgccaag cgtatcaatc acaccgtcaa accgattgac cactttaaaa
     1081 tcaggttcga atctcacccc ggctggaccg tcagtgaatt tccggttggt atcaacccgt
     1141 tgataattca cacgatatcc aattttttca ttgatttgat gttgaaagat cacggcactg
     1201 gagaggaaac ttgaatcccg acgaaagtcc gggtcgcgga ggttcgagat aaagttggca
     1261 ttgccgagtg taaagggttt cccagcttca aacagccgtt gttgatcgag tggtaacgcc
     1321 acggctttga caatgcttcc ggctggtgga aggctggcgc ctggggcatc aaacgaaccg
     1381 tcagtcagtt gagcaaacag ggtattgcct aaaatccggc cagagagggt gatattgggg
     1441 cgaaacgcat actggacaaa tccctgtccg ctccagtttc gatttggggt atcaccgtcc
     1501 acaccatcag aaacattgag gtgcatcaac ccggcactat acagaaaccg atctgacgcc
     1561 gcgcctccgg ctacctgggc tcgaccacga aacagcccca ggctgccgcc ttcgagctga
     1621 atctgacctc gcacggggcc accgccgtgg gccgtcacga cattgaccac gccgccgatg
     1681 gccgtggtgc catagagcgc cgattcgggg ccgcgaacca cttcgacccg gtcggtatca
     1741 acgacaaaca attcctggat ttgagccagt gaatcacctt gagtactggc tgcatcacga
     1801 agtcgcaggc catcaacgac caccgcggtg tcatagctgc gcaatccgcg aataaaaatg
     1861 ccagactggc ttcctggccc accgagttgc tgaacccgga ttccaggcat ttgccggatg
     1921 ctttccgtga tggaaagt
//
LOCUS       DAOQDO010001326         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59500_length_1938_cov_2.890600, whole genome shotgun sequence.
ACCESSION   DAOQDO010001326 DAOQDO010000000
VERSION     DAOQDO010001326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59500_length_1938_cov_2.890600"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PKZ53_18485"
     CDS             complement(<1..1152)
                     /locus_tag="PKZ53_18485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ42484.1"
                     /translation="MLTHLLQSLRIPVVYLDPTLKIGWANQQFVEWARSRDANLEGQP
                     LSDVLDPVIFAQLVQHLRGGLGGETISFKDYVPRNHRVEQVTGTVTPDIDAPGVVQGL
                     SLVLESTFSKPAPHLFLNYAGRMFELTQDPVCLIDPTDQFRFVYVNSAACRHFGLAEP
                     DLLKKTIPDFDPTFSLDKCQFKFESLTGNELTILETTHVNAQGKPIPVEVSCSLIELD
                     GKPFSFSVIRDLTTRIKAEEAIRQNEKIFRELVENLPVGAVYIKGNEIWLNRAAEQLT
                     GYAVSEIGTLKNWFEKLYPGRSQEVEDLYLTDRTSQSRKPVIVPLRRKDGQIKHIEFS
                     GVSLGDNELWLLYDVSEQLATSQALVDQNQLLQTLFDHLPVMAATFDASG"
     gene            1430..1879
                     /locus_tag="PKZ53_18490"
     CDS             1430..1879
                     /locus_tag="PKZ53_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42485.1"
                     /translation="MNSKLLILLNFAATLLMTGIIWQVQLVTYPIMGLVGKSEFMKYH
                     AFHTTWITPVVGPPMLVELITAILLVGTHPEEMPDWAAWVGLGLCGFLFLSTGLISVP
                     LHNQLATGYTERAHSLLVLTNWIRTVGWTLRAALMIWVMARMMSRAI"
BASE COUNT          518 a          457 c          470 g          493 t
ORIGIN      
        1 cccgctggca tcaaacgtcg cggccataac tggcaaatga tcaaataagg tttgtaacaa
       61 ttgattttgg tcaaccagtg cctgggaggt agccagttgc tcgcttacgt catagagcag
      121 ccaaagctca ttgtctccca ggctgacacc tgaaaattcg atatgtttga tctggccatc
      181 tttgcggcgg agcggaacga tcactggctt gcgggattga cttgttcgat ccgtcagata
      241 gaggtcttca acttcctgtg aacggcctgg atagagtttt tcaaaccagt ttttgagcgt
      301 tccgatttcc gaaaccgcgt atccggtcaa ctgttctgcc gctcgattca accagatttc
      361 attgcctttg atataaacgg cacccaccgg caggttttca accagttcgc gaaagatctt
      421 ttcattctgc cgaatggcct cttcagcttt gatccgggtg gtgaggtctc gaataaccga
      481 aaaactaaac ggcttgccat ccagttcaat caaactgcac gagacttcga cgggaattgg
      541 ctttccctgg gcattgacat gggtggtttc caaaatcgtc agttcatttc cagtgagaga
      601 ttcaaacttg aactggcatt tatccaaaga aaaagtaggg tcaaaatctg gaattgtttt
      661 tttcaacaga tccggttcag caagtccaaa atgccgacaa gccgcgctgt tgacatacac
      721 aaaccggaat tgatcagttg gatcaatcag gcacaccgga tcctgggtca attcaaacat
      781 ccgtcccgca tagttcaaaa acagatgtgg cgctggtttg gaaaaggttg attcgagaac
      841 cagactcaat ccttgaacga caccaggtgc gtcaatgtca ggcgttacgg ttccggtcac
      901 ctgctcaact ctgtgatttc gagggacata gtctttaaag gaaatggtct cgccacctaa
      961 cccaccgcgc aaatgctgaa ccagttgggc aaaaatcact ggatcaagca cgtcactcaa
     1021 cggttggcct tcaaggtttg catccctgga tcgagcccat tccacaaact gctgattggc
     1081 ccaaccaatt ttcaaggttg ggtcgagata caccactggg atccggagtg attgcaacaa
     1141 atgggttaac ataggatgaa tcttggaggt aatagccaga aaagccagct acctggcagt
     1201 acatagaaaa tgtgaagaaa ttgccagatt tttgagtgag aactggatgt gcgtgcgggg
     1261 aggagttggc ctgaactggg caacacaata gcaaagatca gttgcactat ccaatcaaca
     1321 cctggaaaag ttataacaaa tttttcgttc caattcagcg acaattttgc ccgcccgggt
     1381 tcattttcta tttttttcgt tcggacgaca ccgttacttt caactcttta tgaacagtaa
     1441 actactgatt cttcttaact tcgcagcgac actcttgatg acaggcatca tctggcaggt
     1501 acaactggtc acgtatccga ttatggggct ggtcgggaaa agtgaattta tgaaatacca
     1561 cgcttttcac accacctgga ttaccccggt ggttggcccg ccgatgctgg ttgaattgat
     1621 cacggcaatc ctgctggtcg gaacacaccc tgaagaaatg ccagattggg ccgcctgggt
     1681 tgggcttggg ctgtgtggat ttttattcct ctcgaccgga ttgatctcgg ttccgttaca
     1741 caatcaactc gcaaccgggt acaccgaacg cgcccattcc ctcctggttt taaccaactg
     1801 gatccgcacg gtgggatgga ccctccgggc cgcactgatg atatgggtaa tggcgagaat
     1861 gatgagtcga gcgatatgag agcaattgag aatgaagaat gaagaatgaa gaatgaagaa
     1921 ttgagaatga agaatgaa
//
LOCUS       DAOQDO010001327         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59511_length_1938_cov_2.356877, whole genome shotgun sequence.
ACCESSION   DAOQDO010001327 DAOQDO010000000
VERSION     DAOQDO010001327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59511_length_1938_cov_2.356877"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKZ53_18495"
     CDS             complement(<1..564)
                     /locus_tag="PKZ53_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42486.1"
                     /translation="MFAQSGCLTAEAPSVPATVSLTVNFADRVIYKVPVKEIAGVINT
                     FAMTSSTPGGPQPSNLDAQTGTVRFQPLQLGKFDFVITASNCSGKSATTTVTMEVKPN
                     PAQPVILDPVYSTRVGLIMEEMIKEGDDDPIFSKIREGATLVVVPPGGTFQTGESFKL
                     ELRRPKGNAPRTPKWQVRLRDNVKSTPG"
     gene            979..1101
                     /locus_tag="PKZ53_18500"
     CDS             979..1101
                     /locus_tag="PKZ53_18500"
                     /inference="COORDINATES: protein motif:HMM:NF023134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNJ42487.1"
                     /translation="MIYFYDTTKNHTWRWCSMSLCGNRAKAAAHYRRKRQDVRP"
     gene            complement(1244..1621)
                     /locus_tag="PKZ53_18505"
     CDS             complement(1244..1621)
                     /locus_tag="PKZ53_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ42488.1"
                     /translation="MNNVTELVDRYIQMCNETDRARRQELIAGLWTETCTFADPMGQA
                     EGLAGLDAMAQGLQDQFPGLKVRRISDVDAHHNRFRFAWEFGPEGGAALAGGVDFGVI
                     VDNRLASITGFFDFAPELAAGHQ"
     gene            1787..>1938
                     /locus_tag="PKZ53_18510"
     CDS             1787..>1938
                     /locus_tag="PKZ53_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ42489.1"
                     /translation="MQSVGTLLREWRTRRRLSQLDLACEANISTRHLSFLETGRATPS
                     REMVIHL"
BASE COUNT          457 a          495 c          510 g          476 t
ORIGIN      
        1 gccaggggtg cttttgacat tgtctcggag gcgaacctgc cattttggcg ttcggggtgc
       61 gtttcctttc ggtcgccgca gttccagctt gaatgattcg ccggtctgaa aggtgccgcc
      121 gggtgggacg accaccagcg tggcgccttc acggattttg gaaaatatgg ggtcgtcatc
      181 gccttccttg atcatttctt ccataatcaa tccgacgcga gttgaataaa ctggatccaa
      241 aatcaccggc tgggcaggat tgggtttgac ctccatcgtg acggtggtgg ttgcactttt
      301 tccagaacag ttggacgcgg taatgacaaa atcaaatttc ccaagctgca acggttgaaa
      361 tcggaccgta ccggtttggg cgtccaggtt gctcggctga gggccacctg gtgtggatga
      421 cgtcatggca aaagtattga tgacaccggc aatttctttg accgggactt tgtaaattac
      481 ccggtcagca aagttcacgg tcagactcac cgtcgctgga acggatggtg cctcggctgt
      541 aagacaaccc gattgagcga acacaacgcc gggcctcacc aaatttgccc cgctctgacc
      601 ggatatcaca gcgcagattc ccaataccaa acccaaaaac cccaatttgc tccatgctgg
      661 tcgaatgcgt gccatcacga cagattcctc ctcactggtt ttttgaaaaa attgactgtg
      721 ctgaagttga ctgctgaagc cagtgtggtg cactggtgag ttccctggaa agaaaagcgg
      781 tgaggaagat actagcctgt ccagggagac tgaagcaagg ctcagaaaga tttcgagctg
      841 aagaaattgg gctcagggtt gaagactcgc aagcttggag ttgatttcct tatcggagtg
      901 ccagttgccg aggcggctgc cgatttgctg agttcgggcg aaccaatgcg ggtcaagaaa
      961 tgcgaagctg atgattgagt gatttatttt tatgacacga ccaaaaacca cacctggcgg
     1021 tggtgttcaa tgagtctctg tggaaatcga gccaaggcgg cagcccatta ccgccgaaaa
     1081 cgccaggacg tccgcccata gcctttcaga taaatatttc tttggagtgc ggcaacttgt
     1141 tgcagctttg gtagacagcg acttgtcgct gaaccacttt ggcctgactc agattgggga
     1201 acgacaagtt gttgcactcc atattttttc tcggaaacac cgattattga tgtccggctg
     1261 ccagttctgg ggcaaaatca aaaaatccag taattgatgc caggcggttg tccacgatga
     1321 ctccaaaatc taccccgcca gccagagcgg cgccgccttc cggcccaaat tcccaggcaa
     1381 aacgaaaccg gttgtgatga gcatctacat cgctgattcg ccgaactttt agcccaggaa
     1441 actggtcctg cagtccctga gccatcgcat caaggccagc cagcccttcg gcctgcccca
     1501 ttggatcagc gaaggtacag gtttcggtcc acaaaccggc aatcagttcc tgtcgccgtg
     1561 cccgatcagt ttcattacac atttgaatat agcgatcaac cagttcggtt acattgttca
     1621 tatcggtctc cattggaaaa tttttaaagg tgtctcccaa ccgcagtggc caggtgtttg
     1681 tctggctgca ttttcctgtt ttggcagatg gaagcaatta cctcacaggt aatgggaacc
     1741 gctcatggaa ccgtggtaag gttgtgaccc tacaggagaa atttgtatgc aatctgttgg
     1801 aacgttgctc cgtgaatggc gcacccgccg ccgattgagt cagcttgatc tggcctgtga
     1861 agccaatatt tcaacccggc acctgagttt tctcgaaact ggtcgtgcca cccccagtcg
     1921 ggaaatggtg attcatct
//
LOCUS       DAOQDO010001328         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59569_length_1937_cov_2.695005, whole genome shotgun sequence.
ACCESSION   DAOQDO010001328 DAOQDO010000000
VERSION     DAOQDO010001328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59569_length_1937_cov_2.695005"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PKZ53_18515"
     CDS             complement(<1..1410)
                     /locus_tag="PKZ53_18515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNJ42490.1"
                     /translation="MKHLSGLDATFLYLETPETPMHVGSMHLYDLPEGFDGDFYEEVK
                     KHVSRRMHLAPVFHRKLALMPFELANPVWVEDDDVDLDYHIRRIMLPKPGTIEQLEAY
                     VGRLHSSLLDRSRPLWEFYVIEGLQSGQVGFYSKVHHAAIDGQAGVAFASAVLDISPI
                     PREVKQPVRRRGVQYQLGVAELIQAALTNVVAQYAKLIWYLPKTAKTLGSALIPEKDA
                     SGRRRFSWPKNLSLGPRTLFNVSITNQRTFGSVSIPLDEAKQIAKAVNVTINDVVLGL
                     CSGAMRRYLEESGYDGVPKKPMVAAVPVSLREAGNTEQNTQATMMLCSLASHIKDPIE
                     RLKAIKSSTSAAKESINNFKAVIPTDFPSFGAPWLMSGLASLYGRSKIADNLPPIANV
                     AISNVPGPPVPLYLAGARMRTYYPVSIIMHGMALNITVESYQGSLDFGLTACRRAIPD
                     VKDLANYIADAHQELKTKVT"
BASE COUNT          469 a          473 c          545 g          450 t
ORIGIN      
        1 tgtcactttg gttttgagtt cctgatgggc gtcagcgata taattcgcca gatctttaac
       61 gtctggaatc gcacggcgac aggcggtgag accgaaatca agcgaaccct gataactttc
      121 caccgtgata ttgagcgcca tgccgtgcat gatgattgaa accgggtagt aggttcgcat
      181 tctggcacct gccaggtaca gcggtaccgg cggacccggc acatttgaaa tcgcgacgtt
      241 ggcaatcgga ggcaaattgt cagcaatttt cgaccgacca tagagcgaag ccagtccgct
      301 catcagccag ggggcaccaa aggacggaaa atcggtcgga atcaccgctt tgaagttgtt
      361 gatcgattct ttggccgcgc tggttgaaga tttaatggcc ttgagccgtt cgatggggtc
      421 tttaatatgc gaagccaggc tacacagcat catcgttgcc tgtgtatttt gctcagtgtt
      481 tccagcttcc cgaagcgaca cgggaacagc cgccaccatc ggctttttgg ggactccatc
      541 atatccgctt tcttccaaat agcgccgcat ggcaccactg cacaagccta aaaccacatc
      601 attgattgtg acattgacgg ctttggcaat ttgtttggct tcatcaagcg gaattgaaac
      661 tgacccaaag gtgcgctgat tggtaattga aacattaaaa agcgttcgag ggccaagtga
      721 aaggttcttt ggccaggaaa aacgacgcct ccctgacgca tctttctctg gaatcaatgc
      781 gctgcccagg gttttggctg tttttggaag ataccagatc agtttggcat actgggccac
      841 gacattggtc agggcggcct ggatgagttc ggccacgcca agctgatact gaactccacg
      901 ccgccgaacc ggttgtttga cttcgcgggg gatcggagag atatcgagca cggcactggc
      961 gaaggcaacc ccggcctgac cgtcaatggc cgcgtgatga accttggaat aaaaaccgac
     1021 ctgacctgac tgcagtccct caatcacata aaattcccag agcgggcggc ttcggtcgag
     1081 cagactggag tggagtcggc ccacataggc ttcgagttgt tcgatggtcc ccggtttggg
     1141 gagcataatc cgccggatat ggtagtccag gtccacgtca tcatcttcga cccagacggg
     1201 gttggccagt tcaaacggca tcaacgccag tttgcgatga aacaccggcg ccaggtgcat
     1261 gcgcctggag acgtgttttt tcacttcttc atagaaatca ccgtcaaacc cttcgggcag
     1321 gtcatataaa tgcatgcttc cgacgtgcat cggggtttcc ggagtttcaa gatagaggaa
     1381 cgtggcatca agtccgctga ggtgtttcat aagtcaaatt ggaggaaaaa gtggttggtg
     1441 gaaccccagc cgggcagtcc agccagcaat ggttcacagg aaattgaagt atgaagaaat
     1501 gagtcggagt tggagtgggc ccaagcgttg gcttcgatta gggcgtgacc gaagcaaact
     1561 gcaggtggtc aatgtactta cagcctaaca cggctgaagt caagtacgca tctggtaccg
     1621 ccgaaaattt cggggtgcgg tggcttgaca ccgctttaac ctggcaggtt gaccttggca
     1681 aaaaacataa acgacataaa gggcgaaaag gacaaaaact cgaaaaccca gaagtgaatg
     1741 atttattttg ccccagcccc cagcccgagg tctttatccc gctttccccg gcggatcggt
     1801 tggaacttca tcagtgagca ccgctttggg aatatgtagc gtcgaggcat ctgcaagttg
     1861 ctgatcggtt agcggtttgg gtgtgatgaa ggtttcagtt ggatgggtcg aaattggctg
     1921 gccgacggtt ggtagat
//
LOCUS       DAOQDO010001329         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59620_length_1936_cov_3.091441, whole genome shotgun sequence.
ACCESSION   DAOQDO010001329 DAOQDO010000000
VERSION     DAOQDO010001329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59620_length_1936_cov_3.091441"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1897
                     /locus_tag="PKZ53_18520"
     CDS             371..1897
                     /locus_tag="PKZ53_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42491.1"
                     /translation="MGLVQRVRSVSQQMAMVFDETKIRRDLDQLEKEVSLNPTNIFTL
                     QQLCNLYQAAGNNEKAVAILCQVAELYRERNEPEFTLAFLRKAERMCLPEQRVNLLCQ
                     IVDLNIQLQKYEDAYQRMVNVLEILIREGKATIALGLVNTLPPLGLKDARYRKELVEY
                     VNLHREEWAQGSKGTWILEESEKPPVEVTSRQLEMKFPNHTLLVVDDEPGVLQLLTMA
                     LKRLGCKIVTASNGEEAYQKAVETCPNLIISDLMMPQMDGSQLFTSLRAHPTLCQVPF
                     VCLTSNSLEKERIAALDRGVEDFWVKPFSLAELVLRVGKLLLRHQKQPDMVGQLTQIA
                     FPELLQLLDHGQKTGLLRLRSEGQEATLYFHQGTLVQAELGPYSGELVAYKLVYWMTG
                     EFSFFSQPVDCQTTIPYSTQQLLIEGLRRFDEANQLIDDVFPDLSKVYGFQFDPQKLL
                     FEPELNPYLARLRAVLNGRNTLQDCCHELSGELEVLILAAELLEQHILVPDLTFDRMD
                     "
BASE COUNT          523 a          486 c          451 g          476 t
ORIGIN      
        1 aaccaaccac ttccaacatt caatcgctca ggaaactttt gacaaggtac ttataccgat
       61 ttgcactgaa gccctcgtct tagaaaacag tcaaataatt caatcaaatg gagttggcaa
      121 agccctgact cctgactcgt gactcctgac tggtataatt tcaaaattct caaaattttt
      181 acgaaacaat ttccgtgtcc tgcccacttt gcatgtgatg gttgaaaacc actcgcaccc
      241 gcagaagctg gcttattcct gccgccagct ctcaaattgc caaagaggag atctgcagac
      301 cgtttccgtt gcttttcaaa tggggtaacc attttcttgc cgcaccagaa aacgactgcg
      361 ccaaaaacat atgggattgg ttcaacgtgt tcgctcagtc agccagcaga tggccatggt
      421 ttttgacgaa acaaaaattc gacgtgatct tgatcaactt gaaaaagaag tttcgctcaa
      481 cccgaccaat attttcacgt tgcagcagtt gtgcaatctg taccaggctg ccggaaacaa
      541 tgaaaaagcc gtcgctatct tgtgtcaggt tgcagaattg taccgggaac gaaacgagcc
      601 ggaattcacc ctggcattcc tgcgcaaggc tgaacgaatg tgccttcctg aacaacgggt
      661 aaacctgttg tgccaaatcg tagacttgaa catccagctt caaaagtacg aagacgccta
      721 tcaacgcatg gtcaatgtgc ttgaaatttt gattcgcgaa ggaaaagcaa ccattgcgct
      781 gggtctggtc aatacgctgc caccgcttgg actcaaagat gctcgctatc gaaaagaact
      841 ggtcgaatat gtcaacctgc atcgggaaga atgggctcaa ggctcaaaag gcacctggat
      901 cttggaggaa tcagaaaaac ctccggtcga ggtgaccagt cggcagttag aaatgaaatt
      961 tcccaaccat acattgctgg tggttgatga cgaacctgga gtgctccagc ttttgaccat
     1021 ggccctcaag cggctggggt gcaagattgt cacagccagc aacggcgaag aagcctacca
     1081 aaaagcagta gagacctgcc caaacctgat tatctctgac ttgatgatgc cgcaaatgga
     1141 tggcagtcaa ctctttacct ccctccgggc ccacccaacc ctgtgtcagg ttccatttgt
     1201 atgcctgact tccaacagcc tggaaaaaga acgaatcgcc gctctggatc gaggggtaga
     1261 agatttctgg gtcaaaccct tcagcctggc cgaactggtg cttcgagttg gaaaactctt
     1321 actccgccat caaaagcagc cggatatggt tggtcaatta acccaaatcg catttcctga
     1381 attgctccaa ctgcttgatc atggtcaaaa aactggtctg ttacgcctgc gttccgaagg
     1441 gcaggaagcg accctgtact ttcatcaagg aacactggtt caggccgaac ttggccccta
     1501 ttccggtgaa ctggttgcat ataaactggt gtactggatg actggggaat tttcgttttt
     1561 ttctcagcca gttgattgtc aaacaaccat tccctatagc acccaacaac ttctgatcga
     1621 agggttacgg cgctttgacg aagccaatca gctcattgac gatgtctttc cggatttatc
     1681 aaaagtgtat ggatttcagt ttgaccctca aaaacttctg tttgaacccg aattaaaccc
     1741 atatctggcg cgcctccggg cagtattgaa tggtcgcaat accctccagg attgttgcca
     1801 tgaactctca ggagaactgg aagtgctcat tctggcggcg gaattattgg aacagcacat
     1861 tctggtcccc gacctgactt ttgaccggat ggattgagga gccagggctg aagaactcgg
     1921 gcttggggct gagggc
//
LOCUS       DAOQDO010001330         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59651_length_1936_cov_2.081340, whole genome shotgun sequence.
ACCESSION   DAOQDO010001330 DAOQDO010000000
VERSION     DAOQDO010001330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59651_length_1936_cov_2.081340"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..904
                     /locus_tag="PKZ53_18525"
     CDS             56..904
                     /locus_tag="PKZ53_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006033624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNJ42492.1"
                     /translation="MIESFPRRIICLTEETTETLYLLGQQDRIVGISGYTVRPPEARK
                     KPKVSAYIHAKFDKILALKPDLVFAFSDLQADIVRELIQRGVTVFTFNQRSVPEILEM
                     ILTVGRIVGCPEQAVGLVQGYQAELEAIRQSASRFPYRPRVLFEEWYEPLISGIRWVE
                     ELIEIAGGDPIFPHLRECKAGKDRILAPETVIPLDPQVIIGSWCGRSLKKNWIRNRTG
                     WDSISAVREKHIYEIKSAYILQPGPAALTEGLRQLHLILAHSVGITVPSELLPQESVD
                     KDLKLT"
     gene            complement(1016..1216)
                     /locus_tag="PKZ53_18530"
     CDS             complement(1016..1216)
                     /locus_tag="PKZ53_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42493.1"
                     /translation="MKSKVVLGLVLASVLGMSGATMAATPYQAKTPTSKVATGGKAGA
                     AKKHHKHHHKKQIKDGATKDKR"
BASE COUNT          527 a          477 c          439 g          493 t
ORIGIN      
        1 caattcctta ttctcgcctg tattccccca agccccaagc cctgagccct aaaatatgat
       61 tgaatccttc ccgcgccgaa ttatctgtct gactgaagaa accaccgaaa ctctgtactt
      121 gcttggccag caagatcgca tcgttggcat ttccggctat accgtccggc cacccgaagc
      181 tcgaaaaaaa cccaaagtgt cggcttatat ccacgccaaa ttcgacaaaa ttctggcatt
      241 gaagccagac ctggtgtttg ccttttcaga tttgcaggca gatattgtcc gggaattgat
      301 tcaacgaggc gtgaccgtct ttacttttaa tcagcggagt gtgccggaga ttctggaaat
      361 gatcctgacg gttggccgaa tcgtcggctg tcccgagcaa gccgtgggtc tggtccaggg
      421 ctaccaggct gaactggaag ccatccgcca atctgcttca agatttccct accggccacg
      481 agtattgttt gaggaatggt atgagccgtt gatttccggc attcgatggg ttgaggaatt
      541 gattgaaatc gcgggaggtg accccatttt tccgcatctg cgggaatgca aagccggaaa
      601 agatcgcatt ttggcaccgg aaacggtcat cccgcttgat ccccaggtga tcatcgggtc
      661 ctggtgtggg aggtcactca agaaaaactg gattcgaaat cgcaccggct gggactcaat
      721 cagtgccgtg cgtgaaaaac acatctatga aataaaatcg gcctacatcc tgcaaccggg
      781 tccggcagca ttaaccgaag gactgcgcca gcttcacctg attctggctc attcggttgg
      841 aatcaccgtt cccagtgaac tcctcccgca agaatcagtt gataaagatc ttaaactcac
      901 ttgaatccaa aagtgagcaa aacaaaaaaa tggtgggcaa gtgacaggag tcttgcccac
      961 cggaattgct cgcacaggaa gaggaattac gcgtacgaca ctgaggagga attacttacc
     1021 gcttgtcctt tgtggcgcca tctttaatct gtttcttgtg atggtgtttg tggtgctttt
     1081 tcgctgcccc agcttttccg ccagttgcaa cttttgaagt cggtgttttt gcctgatacg
     1141 gggttgcagc catggttgca ccactcattc ccaaaactga cgccaaaaca agtccaagaa
     1201 ccactttact tttcataatc aaaaaactcc ttactcaacc agattcaaaa ccgaaaaata
     1261 tcaataaatg gaaaggtttg tgactgatca acaaccagac taaatcaaat gtgccacttt
     1321 tgactcagcc ttcgattctg atgagtgcaa gtctgggacc acaacccaca ctagctggcc
     1381 caaagaaaga acgaaattcc gactccggat caaaattaaa tacaactcat tgattttaaa
     1441 caaactatca aatttgtttt tttctggaaa atgagaatac agctcaattt ttatgaaatt
     1501 tggctgagta ttcacgtgat ttggcaggtt tgggcgtgtc acttctgtct gttagaccaa
     1561 attgccacag tcaattggtc ctgacagaca ccgccttgct tcaggataat gaggctggaa
     1621 aagcgatttc aaaaaaataa aattttttcc agaaaaatct tcaggtatct gtaatcggcc
     1681 tttttccagc ttcatagaac aagcatctct cctaaagtac agcgcagagt gaattggtcg
     1741 ttgatggagt tgagtgcgca cagggtgtca ggcgtcagtt ggccggaact gaatccagaa
     1801 cgggtcacct ggcacctggc atcctggttg actcaatttg actcagcgac aatcaccgac
     1861 ctgtaccgac caatccacac ttgaaaggag ccaccaggct atgacgcacc gaattttagc
     1921 aattgtcgga atagcc
//
LOCUS       DAOQDO010001331         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59687_length_1935_cov_2.761702, whole genome shotgun sequence.
ACCESSION   DAOQDO010001331 DAOQDO010000000
VERSION     DAOQDO010001331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59687_length_1935_cov_2.761702"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..772
                     /locus_tag="PKZ53_18535"
     CDS             131..772
                     /locus_tag="PKZ53_18535"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNJ42494.1"
                     /translation="MRLAHTPTALTDLRTPTSTEPLRILISGCMMGLPCGVDGSDNGM
                     GGVLTDLVTLPTVKVFSFCPEDHALGTPRTLPDIHGGDGSDVLDGKARVLDEHGEDLT
                     EGMIRGAHAMLSFAQAHQIELAILTDMSAACGSQVISDGCRLDTDRKYQMGVGVSTAL
                     LLRNGFPVVSQRDFRTLGKIRMRLEPGFSPDPEALDHHEKEWYQTYFQTSPPQ"
     gene            874..1512
                     /locus_tag="PKZ53_18540"
     CDS             874..1512
                     /locus_tag="PKZ53_18540"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ42495.1"
                     /translation="MLIFDHLVLVAPTLEEGAQYVESQLGVRPFPGGQHRELGTHNLL
                     LRIGQDCFLEVIAVDPSATLHQGPRVFGLGNQASVEADWLAGNRLKTWVGRTTQLETI
                     VSQYGSLVGEIKSVSRGERRWRMTIPADGSLPQGGSIPTSAEYEPAMIPAHHMPDAGC
                     SLQQVIVHAPDGETVRQLYARLGVLGPVTIETGSPASLRALIQTPNGIVELH"
     gene            complement(1593..>1935)
                     /locus_tag="PKZ53_18545"
     CDS             complement(1593..>1935)
                     /locus_tag="PKZ53_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ42496.1"
                     /translation="ELVLHIAAWENVTVRRLEGHSTSEPEEGDFPPVSDTSEAAWHNT
                     VTRLNDIQARFLNEVARLTDAGLNEKVVGENYSIRQLLRATARHKIYHTAQISLLRKG
                     WTAEIGKTQAG"
BASE COUNT          445 a          521 c          506 g          463 t
ORIGIN      
        1 tcaacacaat caggtgccag ccaataagcc gattgaaacc tggaccgact ccgcgataga
       61 gtgctggttc agttctcaat ttccaatcct ttccaatcca aattttccac atttttgtga
      121 ggaggaccca atgcgactgg cccacacccc cacggccctg actgatcttc gaaccccaac
      181 ttccaccgaa ccgcttcgaa tacttataag tggctgtatg atgggacttc cctgcggcgt
      241 tgacggctct gacaatggga tgggtggcgt actgaccgac ctggttaccc tgccaaccgt
      301 gaaggtgttt tcattttgcc ctgaagatca tgcactcggc acaccgcgaa ccctgccgga
      361 tattcatggc ggtgatggca gtgatgtgct ggatggaaaa gcgcgggtgc ttgatgaaca
      421 tggagaggac ctgaccgaag gcatgatcag gggcgcgcac gcgatgctca gttttgccca
      481 ggcccatcag atcgaactgg caattttgac cgatatgagc gccgcctgcg ggagtcaggt
      541 aatttcagat ggctgccgat tggacacaga ccgcaaatac cagatggggg ttggtgtttc
      601 gaccgcgctg cttttgagaa acggctttcc agtggttagc cagcgggact ttcgaactct
      661 gggaaagatc cgaatgcggc tggaacctgg cttttcaccg gacccggagg cgctggatca
      721 tcacgagaaa gaatggtacc aaacttattt ccaaacctcg ccccctcagt aatgccattt
      781 aggggtcagg ggtcagggaa atacagtttc ttcaattatt tagcctttcc gttatacaag
      841 aacttcaatc caaaatggtt agcaattctc actatgctca tatttgatca tctggttctg
      901 gttgctccga cgcttgagga aggcgcccag tatgtcgaat cacaacttgg cgtccggccc
      961 tttccaggtg ggcagcaccg cgaactcgga acccataacc tgttgcttcg aattggacaa
     1021 gactgttttt tggaagtgat tgccgttgat ccgtcagcaa cgttacatca ggggccacga
     1081 gtttttggct tgggcaatca agcatctgtc gaagctgact ggctggctgg aaaccgactc
     1141 aaaacctggg ttggtcgaac aacccagttg gaaaccattg tgagtcaata cggttctctg
     1201 gttggtgaaa tcaaatcggt cagtcgcggt gaacgacgct ggagaatgac cattccagcc
     1261 gatggttcat tgccacaggg cgggagcatc ccaacttcag ccgaatatga accagcaatg
     1321 attccggctc accatatgcc ggacgccggg tgctcgctcc agcaagttat cgtgcacgct
     1381 ccggatggtg aaaccgtgcg tcaactatat gcccgacttg gtgtcttggg cccggttacc
     1441 attgaaactg gatcgccagc gagcttgcgg gcactgatcc aaactccaaa cgggattgtt
     1501 gaactccact aaatcgcctg agaccagtga tgcggacaag gaagcaaagt gaccatctga
     1561 caaccgtccg tcactttgcc tccttgtctc cttcacccgg cctgggtctt gccaatctca
     1621 gcggtccagc ctttgcgcaa gagactgatt tgcgccgtat ggtaaatctt atgccgggcc
     1681 gttgcgcgca gcagttgtcg gattgaatag ttttccccca cgactttttc atttaaaccc
     1741 gcgtccgtca gacgagcaac ttcgttgaga aagcgcgcct gaatgtcgtt taatcgggtc
     1801 accgtattgt gccaggcagc ctcactggtg tctgaaaccg gtgggaaatc gccttcttcc
     1861 ggctcagaag ttgaatgtcc ttccaaccga cgaacagtta cattctccca ggcggcaatg
     1921 tgcaggacca gttcc
//
LOCUS       DAOQDO010001332         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59697_length_1935_cov_2.375000, whole genome shotgun sequence.
ACCESSION   DAOQDO010001332 DAOQDO010000000
VERSION     DAOQDO010001332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59697_length_1935_cov_2.375000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..>1935
                     /locus_tag="PKZ53_18550"
     CDS             190..>1935
                     /locus_tag="PKZ53_18550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNJ42497.1"
                     /translation="MDIRLRIVLCFFGIMLTAWFLNPAVSSHTATPFSVVTPTPVEAS
                     SSLPYHQSLVFKTDLSKSSLSPTVRASTSTLVRDLTGDGQADVIQLVNGRLSIRPGGG
                     DATFGDATLLDPGHTASLLAVSDANETGQPVVLVLHTTGQLEAFQISWTSSESRLQPD
                     VSSVATFSTLAGGRSLTVSDVNHDGYDDIVVAADHQVMVCLGSLEGYAVSQYLSLPFD
                     GVIEVDLHSGRLLAFDELSQVIHFWNWQDGELRPGRDLSLPAQRAVVTTGDVNLDGHS
                     DVLAATDDGQLTLWLGDEAGEFTFSRQFPVVTGITRMEWGHLDLDGLPELVFETATGQ
                     LGVIWSDATDMFNHLQLLKLSEPVETWAVGELDGDTLTDLVLDGSTSLKIFVSQQKAA
                     LLVTTTDDAGPGSLREAILGATAAGGANTIAFNIPGPSSGPFVIKLATALPQIPRGTV
                     IDGTTQASNSKIDPNPNGPDIFIDATTAGVAIGFNLTTGQNIIRGIGIFGANGVAIQI
                     SGVSATGNRVEGCYLGTNADASAAAVRGNNTGILINAGATNTAIGGASASARNIISGN
                     SGSGVVLDGTATRSNL"
BASE COUNT          420 a          472 c          520 g          523 t
ORIGIN      
        1 tatttcatta taccaatttg caacccagtc ctcagtatta gggcacagtc aaacgagtca
       61 atcaaataga cttagccgac tgctgactgc tgactgctga ctacaaactg gtattacatt
      121 cagccgtagc cagaggttac ttagcaagat ttcttatttt catttgtttt tataaggaaa
      181 atttgtgtta tggatattcg tctccgaatt gttttatgtt tcttcggaat catgcttact
      241 gcctggttcc tcaacccggc ggtttcaagt catactgcca cgcctttctc agtcgtgacg
      301 cccacacctg ttgaggcgag ttccagtttg ccatatcacc agtctctggt tttcaaaact
      361 gatttatcaa aatcctcttt gtcgccaacc gtcagagctt ccacttcgac actggtcaga
      421 gaccttaccg gagatggtca ggctgatgtc atccaactgg tcaatggacg tctgtcaatt
      481 cgtcctggag gtggagatgc aacgtttggt gatgccaccc tgcttgatcc tggtcacacg
      541 gcgtcacttc tggctgtcag tgatgccaac gagactggtc aacctgtggt gctggtgtta
      601 cacaccactg gtcaactcga agcttttcag atttcctgga cctcatctga atccaggttg
      661 caacccgacg tgtcatcggt ggctactttt tcaacgctgg ctggtggtcg ttcactgacg
      721 gtgagtgatg tgaaccacga cggctatgac gacattgtgg ttgcggctga ccatcaggtg
      781 atggtttgcc tggggagttt ggaaggatat gccgtctctc agtatctcag cttgccgttt
      841 gatggggtga ttgaggttga cctgcacagc gggcggttgc tggcctttga cgagttgagc
      901 caggtgatcc atttctggaa ctggcaagat ggtgaactcc gacctgggcg cgacctttca
      961 ttgccagcac aacgggcagt tgtcaccacc ggcgatgtaa atttggatgg gcattctgac
     1021 gtcctggctg caactgatga cggccagttg acgctctggc tgggtgatga agctggcgaa
     1081 tttaccttct ccaggcagtt tccagtggtg actgggatta cccgaatgga atggggccac
     1141 cttgacctgg atggtcttcc cgagctggtg tttgagacgg caactggtca gttgggggtg
     1201 atttggagtg atgcaactga catgttcaat catctgcaac tcctcaaact atcagaaccc
     1261 gttgaaacgt gggcagtggg tgagcttgat ggcgatacat tgactgatct ggtacttgac
     1321 ggatccacct cgctcaaaat ctttgtttca caacagaagg cggctctttt ggtgactacc
     1381 actgatgatg ccgggcctgg ttcgctgcgg gaagccatcc tgggagccac cgccgccggc
     1441 ggggccaata ccattgcctt taacattcca ggtccatcaa gcgggccgtt tgtgatcaaa
     1501 ctggcgacgg ccttgccgca gattccacgc ggaacagtta ttgatggcac cactcaggcg
     1561 agtaattcca aaattgatcc caatccaaat ggaccggata tttttattga tgcaacgact
     1621 gctggcgtcg ctatcggatt taatctgaca actggacaaa atatcattcg gggaattgga
     1681 atctttggcg cgaatggtgt cgcgattcag atttcaggtg tcagtgccac cggaaaccgg
     1741 gttgaagggt gttatcttgg aacgaatgcc gatgcttcag ccgccgctgt ccggggaaac
     1801 aataccggga ttttgatcaa tgccggtgcg accaacactg ccattggcgg ggcgtctgcg
     1861 agtgcccgga acattatttc aggaaattca ggatccggtg tggtgttaga tggtaccgcg
     1921 acccggtcca atctg
//
LOCUS       DAOQDO010001333         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59894_length_1931_cov_3.333156, whole genome shotgun sequence.
ACCESSION   DAOQDO010001333 DAOQDO010000000
VERSION     DAOQDO010001333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59894_length_1931_cov_3.333156"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1931
                     /locus_tag="PKZ53_18555"
     CDS             <1..>1931
                     /locus_tag="PKZ53_18555"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42498.1"
                     /translation="VHRHITQTLISLGATYNQAGDFDQAEQYFKRALANLEKTALSDN
                     PLMINCLNNLATLYKRKGELPNATACFQRALAMAEKQVGRQHPGTATILSNLGVLSFD
                     ARDFGQAERLHQESLTIREHLLGPDHSDVVNTLDHLTSLYQLRGDLDLAIRYRIQCNE
                     AAERNIARNLNSGSERQKMIFLQKTAHFLHQTISLHQHEAVQNQVAANVALTEIVRRK
                     GRALDAMTSTIATLRSQSDSETQRLLDEYAALVSQISTLTLSEAKGKPFNNLQDFLKD
                     LNRRQETLEIELSRRSAEFKAQTFPVTLEAIQKLIPPDAVLIEFAVYYPWNVPLQKYD
                     KLRYVAYTLTQDGAIQSADLGEAEPIDAAISEFRKVLGTSHVVLGTNRDLGQPLTQKT
                     KVPTVLAQARRVDQLVMSPVRKLIGTARHLLISPDGALNLIPFSALVTEKGKYLVEEH
                     LITYLTSGRDLLRLQVKIPTHQSALVVADPDYAEGQGPILAGRAMKPLTPLPGTGREG
                     KDIQPLIQGSRLVLQTEATKEVVQKTRRPEILHIATHGYFLEDTARDRSEAGTRVLER
                     VEFQEEKPITIQNPLLRSWLFFAGANRDQAAERSGIMTALEASQLDLWGTKLVVLSAC
                     ETGVGEIRNGDGVFGLRRA"
BASE COUNT          493 a          502 c          506 g          430 t
ORIGIN      
        1 ggtacatcgg cacatcacgc aaacgttgat cagtttgggg gctacctaca atcaggccgg
       61 agatttcgat caagccgaac aatacttcaa acgggcgctg gcaaacctcg aaaagacggc
      121 tctttcagat aacccattga tgataaattg tttaaacaat ctggccacct tgtacaaacg
      181 caaaggcgaa ctgcccaacg cgaccgcttg ttttcaacgt gcgctggcaa tggccgaaaa
      241 gcaggttgga cgccagcacc caggtacggc cacgattttg agtaacctgg gtgttttatc
      301 gtttgatgcc agggactttg gtcaggctga gcggcttcat caagaatcac tgactattcg
      361 ggagcacctg cttgggccgg accactcaga tgtggtcaac accctggatc atctcaccag
      421 tttgtatcag cttcgtggtg accttgattt ggcgatcaga taccgaatcc agtgcaacga
      481 agctgccgag cggaatatcg cccggaactt aaactcgggg tctgagcgac aaaaaatgat
      541 ctttctccaa aaaacagccc attttctgca tcagaccatt tctttgcacc aacacgaggc
      601 cgtccagaac caggtggccg ccaatgtggc gctgaccgag attgtgcgcc ggaaaggtcg
      661 ggctctggat gccatgacca gcaccattgc cacgctccga agccagagcg atagcgaaac
      721 tcagcggttg ctggatgaat atgcagcact ggtcagccag atttccacgt tgacgcttag
      781 tgaagcaaag gggaagccat tcaataactt gcaggatttc ctcaaggacc tcaaccgtcg
      841 ccaggaaaca ctggaaattg aactcagccg acgtagcgcc gagtttaaag ctcaaacttt
      901 cccagtcacg ctcgaagcaa tccaaaagct gattccacct gatgccgtgc tgattgaatt
      961 tgcggtctat tatccgtgga atgttcctct ccaaaaatat gacaaactcc ggtatgtagc
     1021 ctataccctg acccaggatg gtgccattca gagcgctgat ttaggtgaag ctgagccgat
     1081 agatgctgct atttccgagt tcagaaaggt tttgggtact tcccatgttg ttcttgggac
     1141 caaccgggat ttgggccagc ccctgactca aaaaacgaag gtgccgaccg ttctggccca
     1201 ggctcggaga gttgaccagt tggtgatgtc accggttcgg aaattgattg gcaccgccag
     1261 gcatctcctg atttcaccag atggggccct gaatctgatt ccattttcag cgctggtcac
     1321 cgaaaaagga aaatatctgg ttgaagagca tttgattacc tacctgacca gcggtcgtga
     1381 tttactgcgc ctgcaggtca aaatccctac acaccaatcg gcgctggttg tggccgaccc
     1441 cgattatgct gaaggtcagg gaccgatact ggctggtcga gccatgaaac cactgacgcc
     1501 gttgcctgga accgggcgtg aaggcaaaga cattcagccc ctcattcagg gaagccggct
     1561 ggtactgcag actgaagcca caaaagaagt cgtgcaaaaa acgcgccggc cagaaatcct
     1621 gcatattgca acccacgggt atttccttga agatacggcc agagaccgat ctgaagccgg
     1681 gactcgggtt ctggaacggg ttgaatttca ggaagagaaa ccaatcacca ttcaaaatcc
     1741 gctgctgcgt tcctggctct tctttgccgg tgccaaccgc gaccaggctg ccgaaagaag
     1801 cggaattatg accgcgttgg aagcttcaca acttgattta tgggggacaa aactggtggt
     1861 cctttcagcc tgtgaaaccg gagtgggtga aatcagaaat ggggatggtg tttttggctt
     1921 gcgccgggca c
//
LOCUS       DAOQDO010001334         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59961_length_1930_cov_3.060800, whole genome shotgun sequence.
ACCESSION   DAOQDO010001334 DAOQDO010000000
VERSION     DAOQDO010001334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59961_length_1930_cov_3.060800"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1930)
                     /locus_tag="PKZ53_18560"
     CDS             complement(<1..>1930)
                     /locus_tag="PKZ53_18560"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNJ42499.1"
                     /translation="RVYISGGPNFGFDLCTRKVTEAEKDTLDLSCWQVAFNGAEPIRP
                     ETIEAFSNAFARCGLRPETLYPCYGLAEGTLIVAGGLKDQPVVTKTILSEAFKQNQIV
                     STEPAEQGTQTLVGCGQQLFEQRLLVVNQTTLTPCQNLEIGEIWVGGKTVAHGYWNRP
                     EDTAATFQAFLADTGEGPFLRTGDLGFFDQGELFVTGRIKDLIIVHGQNHYPQDIELT
                     VEQCHPALRAGSGAAFTVDVAGSEQLVVVQELEFRQKPDLADVFATIQEAIAQGHDLQ
                     CHAIAIIKPGSIFKTTSGKIRRKATQAAFLASELELVPDGLWQANLEQVATEVLLDAP
                     PLPSLQSAGNLSQPGRDIAHWLINRIATLAKIPASEVDVRQSFARYGLSSVDAVALAG
                     ELEDWLGIPVSPTLVWDHSTIESVAHFLASDPLPTLKPSDEKELPESDLIAVVGIGCR
                     FGGGIDSPDAFWDALRTDVDTVTEVPPSRLERGTFLEPDQSVATDSRWRFGSFLDRID
                     EFDARFFGITPREANRLDPQHRLLLEITWEALEQAGIAADRLAGSQTGVFIGISTTDY
                     SRLSLAEQSQLDPYVGTGNALSIAANRISYLFDLKGPSMAIDTACSSSLVAIHQACQS
                     LRLGESTLAIVGGVNLMIL"
BASE COUNT          448 a          511 c          541 g          430 t
ORIGIN      
        1 cagaatcatc aggttgacgc cgccgacaat cgccagggtt gactcgccaa ggcgcaggct
       61 ctgacaggct tgatgaatgg caaccagtga tgacgagcag gccgtgtcaa tcgccatact
      121 cggccctttg aggtcaaaga gatatgaaat ccggttggcc gcaatgctca aggcgttgcc
      181 ggtgccgacg taggggtcaa gctgcgattg ctctgccagc gagaggcgac tgtaatcagt
      241 ggtactgatg ccgataaaca caccggtttg cgagccagcc agccggtcag cggcaatccc
      301 agcctgttcc agggcttccc aggtgatttc gagcaagagc cggtgctggg gatccaggcg
      361 attggcttca cggggagtga taccaaaaaa tcgggcgtca aattcgtcaa tccggtctaa
      421 aaaactgcca aatcgccacc gtgaatcagt tgccacagat tgatctggct caagaaacgt
      481 tccgcgttcg aggcggcttg ggggaacttc ggtcacggta tcaacgtccg tgcggagtgc
      541 gtcccaaaat gcgtctggcg aatcaatccc gccgccaaac cgacagccaa tgcccaccac
      601 ggcaatcaaa tcagattcag gaagttcttt ttcatcagag ggtttgagtg tgggcaaggg
      661 gtcactggcc agaaaatgag cgacagattc aatggtcgaa tggtcccaca ccagcgtggg
      721 tgaaaccgga ataccgagcc aatcttcaag ttcgccggcc agcgccacag catctaccga
      781 agaaaggccg tatctggcaa atgattgtcg gacatcaact tctgaggccg gaattttagc
      841 taacgtcgca atccgattga tcaaccagtg agcaatgtca cgccctggct gtgacaagtt
      901 tccggctgat tgaaggctgg gaagtggggg ggcatccaga agtacttctg tcgcaacctg
      961 ttccagattg gcctgccaca ggccatccgg cacaagttcg agttcactgg ccaggaaagc
     1021 ggcttgtgtc gctttgcgac ggattttccc gcttgtggtt ttgaaaatac tgcccggttt
     1081 gatgattgca attgcgtggc attgaaggtc gtgcccctgg gcaatcgctt cctgaatggt
     1141 ggcgaagacg tcagccagat caggtttttg acggaattcc agctcctgaa ccaccaccag
     1201 ttgttcactt ccggccacgt ctactgtaaa tgccgcgccg ctgccagccc ggagcgccgg
     1261 atggcattgt tcaaccgtga gttcaatatc ctgtgggtaa tgattctggc cgtggacaat
     1321 gatcaggtct ttgattcgac ccgtgacaaa gagttctccc tggtcaaaaa agcccaaatc
     1381 gccggttctc aaaaacgggc cttcccccgt gtcagccaga aatgcctgaa aggtggcggc
     1441 ggtgtcttcc gggcgattcc agtacccgtg ggcgacggtt tttccaccga cccagatttc
     1501 accgatttca aggttctgac agggggtgag tgtggtctga ttgaccacca gaaggcgttg
     1561 ctcaaacaac tgctgcccgc atccaaccag agtttgggtt ccctgttccg ctggttcggt
     1621 tgaaacaatc tgattttgtt tgaaggcttc ggaaagaatc gtcttggtaa caaccggctg
     1681 gtctttgagt cccccagcca caatgagggt tccttcagcc agtccatagc acgggtagag
     1741 tgtttcaggt cgtaacccac agcgggcaaa agcgttggag aaggcttcga tggtttccgg
     1801 gcggatgggt tctgccccat taaacgcgac ttgccagcaa ctcaaatcaa gtgtgtcttt
     1861 ttcagcttca gtgacctttc gagtgcacag gtcaaaacca aagttggggc cgccggaaat
     1921 ataaacccga
//
LOCUS       DAOQDO010001335         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_59967_length_1930_cov_2.833067, whole genome shotgun sequence.
ACCESSION   DAOQDO010001335 DAOQDO010000000
VERSION     DAOQDO010001335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59967_length_1930_cov_2.833067"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKZ53_18565"
     CDS             <1..522
                     /locus_tag="PKZ53_18565"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNJ42500.1"
                     /translation="ARGLLLIDQHVAHERVLFEQHLRRLMSREVVSQKLLIPHTLDLT
                     PAQAAAFEHTMTDLDQCGFDVMQLSGRTIALRALPAELEPEEARTLLIELLDVVEEEQ
                     RSVALEHFQREIAAGLACRAAIKVNKVLNLEKMTWLIDELMRTENPTNCPHGRPVLLR
                     FDMQDIERGFKRI"
     gene            806..1861
                     /locus_tag="PKZ53_18570"
     CDS             806..1861
                     /locus_tag="PKZ53_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42501.1"
                     /translation="MTFEAQRRQLLSPTWLRLVVVTTIFSIGLVSSGCITSRVALPRE
                     LPVQPAVPTSELVSRITTLQQITQLKSSVGLQFADYKESDQGKGKIYPTADGKLVLQR
                     PQRIRLAVDATLVGKIADMGSDGEQFTVAVFYPTDKRSFIRGTNLRKYTQQVNQLNSS
                     TSTKDISTFSKLRPQHLALPMLVPALPEASPDTSFFVVESRQDEAEVISRDGQMSLEV
                     PAKRVMRTYYVLYVSHRSADGQWVPIYSYWFDRTRKGTPLVRLQIYEEDGTLSTQSEY
                     EGYADKSGRSQLLPQTVKISRPHDGYAVRITFREPEINSDPLSADVFMLQNDENLQVL
                     DLDTEPNSAIRAKASEN"
BASE COUNT          485 a          494 c          477 g          474 t
ORIGIN      
        1 gcccgtggtt tgttgctcat tgatcagcac gtagcccacg aacgcgtttt gtttgaacaa
       61 catctgcggc ggttgatgag ccgcgaagtg gtttcacaaa aactgctcat tccacacacg
      121 ctggatttga caccggctca ggctgcagcc tttgaacaca ccatgactga tctggatcag
      181 tgtgggtttg atgtcatgca gctttcagga cgaactatcg cgctgcgggc gctcccggct
      241 gaactggagc ccgaagaagc ccggacgctg ctgattgagt tgctggatgt ggtcgaagaa
      301 gaacaacgct cggttgcgct cgaacatttt cagcgtgaaa ttgccgccgg tctggcctgt
      361 cgcgccgcca tcaaggtgaa taaggtcttg aaccttgaga aaatgacgtg gttgattgac
      421 gagttgatgc ggacagagaa ccccacgaat tgtccgcatg gtcggccagt cctgcttcga
      481 tttgatatgc aggatattga gcgcggtttt aagcgaattt aacccagatt ttttcgatga
      541 cacaaacaac gaaaatcaca ttttttcaat caacaggcca cgccttggaa atgaaagcct
      601 ggatttcgga ttcagaatga tgccctcagt tgtgggtctc cccgtcgagt tgacacctgt
      661 cagcctgctg gcgaggtttt aactgaattg acaatttgaa catccgttgg taggctcgct
      721 gctttgctct tggatcgtcc aaatctacct tatacccata acgaagcatc atcattaaga
      781 ttaagcagga caggttaata tcagaatgac tttcgaggca cagagacggc agcttttaag
      841 tcccacatgg ctgcgactgg ttgttgttac cacgatcttt tcaattgggt tggtatcctc
      901 cggctgtatt acttcccgcg ttgccctccc ccgtgaactc ccggttcaac ctgcggttcc
      961 cacgtccgaa ctggtctccc gtatcaccac gcttcagcaa atcacgcaat taaaatcttc
     1021 agttggactt cagtttgctg actataaaga atccgatcag ggaaaaggga aaatctatcc
     1081 gacggcggat ggaaaactgg tcttacagcg gccacaacga attcgactgg ccgttgatgc
     1141 caccctggtt ggaaaaattg ccgacatggg gtctgacgga gagcagttta cggtggccgt
     1201 gttttacccg acggataaac gaagttttat ccgcggcacc aacctccgca aatacactca
     1261 gcaagtcaat cagttaaact catccaccag taccaaggac atcagcacat tctcaaaatt
     1321 gagaccgcag cacctggctt tgccgatgct ggtgccagcg ttaccggaag cttcgccgga
     1381 caccagcttt tttgtcgttg aatcacgtca ggacgaagcc gaagtcatct cacgtgatgg
     1441 tcaaatgtca cttgaagtcc cggccaaacg tgtgatgcgc acctactatg ttctgtatgt
     1501 gtcacatcgt tcggctgacg gccaatgggt cccaatctat agttactggt ttgatcgaac
     1561 acgcaaaggc accccgctgg tccggctaca gatttatgaa gaagacggca cgttgagcac
     1621 tcagtcggaa tatgaaggct atgctgacaa atctggccgg tcacaactgt tgccacagac
     1681 cgttaaaatt tcacgcccgc acgatgggta tgcggttcgc attaccttcc gcgagccgga
     1741 aatcaacagt gacccgctct cagccgatgt ctttatgctg cagaacgatg aaaacctgca
     1801 ggttctcgac ctcgacaccg aaccaaacag cgcaattcgt gctaaagctt cggaaaatta
     1861 gtcagtagtt gataagtcag tagtcagtag ataaaacccg gatcgttgaa tccgtggaat
     1921 tcttgaaaag
//
LOCUS       DAOQDO010001336         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60106_length_1928_cov_2.573412, whole genome shotgun sequence.
ACCESSION   DAOQDO010001336 DAOQDO010000000
VERSION     DAOQDO010001336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60106_length_1928_cov_2.573412"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /locus_tag="PKZ53_18575"
     CDS             <1..1875
                     /locus_tag="PKZ53_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42502.1"
                     /translation="AIETVSVLSSPYSAEYGKFSGAVTTIGTRAGGNTWRFLFTNFIP
                     RMRRRTDPETGKSKLVGLESITPRLIFMGPIIKDKLTISQSFQYQFVRNIVPSLPNLK
                     NDVQYESFDSFTQLDWNINERNHLTGVLSLYPQNLQSVNLNTFNPIEVSANFRQRGFF
                     ASAAERAVFTNGSLLETTFAAKRFNAFVFPQGDTPMTVAPAVNSGNFFNRQDRYSTRY
                     DGQSIYSFAPVEAKGNHLFKVGATLSYTTFDGRDRNSRIDVVRQDGTLSQRIEFVGDG
                     QFERNVGETSLFFQDKYTVRPNIVLDLGLRYDRNGLSNQNNVAPRFGFVIVPFSGGRT
                     VIRGGIGVFYDKVPLGIGVFDRFQSRRVTAFQSDGITPTGPAETLLNVFAGELRTPYS
                     VSGTFEVDHQLFKRFFVRFGYQKREGKNEFLVNPEVDPRRGPLLVMSNGATSSYREYQ
                     ITSRYRFNDVSDISFSYVRSKTTGDLNDFNTYFGNFRAPIIRDNQRSRQPFDTPNRFL
                     TVGNFELPFGVVASPVLDIHTGFPFSRVDNDQNFVGIRNPDDQRFLRFLALDLQVTKK
                     IAIPFRGKKLITRIGVKIFNLTNHFNPRDVQANTGSPMFGEFYNSIGRTFRGKFEFDF
                     "
BASE COUNT          453 a          498 c          467 g          510 t
ORIGIN      
        1 gcgattgaaa cggtgtcggt gctctccagc ccctattcgg cagaatatgg aaaattcagc
       61 ggtgccgtca ccaccattgg cacccgcgcc ggcggcaata cctggcgatt tttgtttacc
      121 aactttattc cccggatgcg ccgccgaacg gatccggaaa ccggcaaaag caaactggtt
      181 gggcttgaat ccattacccc ccgcctgatt tttatggggc cgatcatcaa agacaaactg
      241 acgatttcac aatcttttca gtatcagttt gtgcgcaata tcgtgccctc acttccaaat
      301 ttgaaaaacg atgttcagta cgagtcattt gactcattca cccagcttga ctggaacatc
      361 aacgaacgaa accacttaac cggtgttttg tcgctttatc cgcagaattt gcaatccgtc
      421 aatctcaaca cctttaaccc aattgaagtc tcggccaact ttcgtcagcg gggctttttt
      481 gcctcagccg ccgagcgggc tgtctttacc aatggctcac tgcttgaaac cacctttgcc
      541 gcgaaacggt tcaacgcttt tgtgtttcca caaggagaca cgccaatgac cgttgcgcca
      601 gcggtgaaca gcgggaattt ctttaaccgc caggatcgct attcgacccg gtatgacggg
      661 cagtcaatct acagctttgc gcctgttgaa gccaaaggga atcacctgtt caaagtcggt
      721 gcaaccctga gttacacgac atttgatggc cgggatcgca acagccggat tgatgtggtc
      781 aggcaagatg gcacactcag ccagcgaatt gaatttgttg gtgatggcca gtttgaacgc
      841 aacgttggcg agacctctct tttctttcaa gataaataca ccgttcgccc aaatatcgtg
      901 cttgatttgg ggttgcgcta tgaccgaaac gggctctcaa accagaacaa tgttgcacca
      961 cgatttggat ttgtgattgt gcctttctca ggcggaagaa cggttatccg gggcggcatc
     1021 ggggtgtttt acgacaaagt accactgggc atcggtgtgt ttgatcggtt tcagagccgg
     1081 cgggtgacag cctttcagtc agatggcatc accccaactg gcccggctga aacattgctc
     1141 aacgtatttg ccggcgaatt gcgcacacca tacagcgttt caggcacctt cgaagttgac
     1201 catcaactct tcaagcgctt tttcgtgcgc tttggatacc agaagcggga aggaaaaaat
     1261 gaattcctgg tcaatccaga agttgaccca cggcgggggc cattgctggt gatgtcaaat
     1321 ggcgcgacgt cgagctatcg tgagtaccag attacctcgc gttaccgctt taatgatgtg
     1381 agtgatatct cattttcgta tgtccggtcg aaaacaactg gtgatttaaa tgatttcaac
     1441 acctattttg gaaacttccg ggcaccgatt attcgcgaca accagcgctc tcgtcagcca
     1501 tttgacaccc ccaatcgctt tctcacggtc ggtaattttg agcttccctt cggagttgtc
     1561 gcttcaccgg tattggatat tcacactgga tttccgttct cacgagtgga taatgaccag
     1621 aactttgtcg gcatccgcaa cccagatgac cagcgattcc ttcggttttt ggcgcttgat
     1681 ttacaggtaa ccaaaaaaat tgctattccc ttccggggca aaaaactgat tacccggatt
     1741 ggtgtcaaaa tctttaacct gaccaaccat tttaatcccc gtgatgtgca ggccaatact
     1801 ggaagcccaa tgttcggaga gttttacaac agcattgggc gtactttccg gggtaaattc
     1861 gagtttgatt tttaagaacc gggttccggg ttccgggttc cgggttccgg gttccgggtt
     1921 ccgggttt
//
LOCUS       DAOQDO010001337         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60216_length_1926_cov_3.104222, whole genome shotgun sequence.
ACCESSION   DAOQDO010001337 DAOQDO010000000
VERSION     DAOQDO010001337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60216_length_1926_cov_3.104222"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1363)
                     /gene="zigA"
                     /locus_tag="PKZ53_18580"
     CDS             complement(122..1363)
                     /gene="zigA"
                     /locus_tag="PKZ53_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HNJ42503.1"
                     /translation="MSAKPKLPVTVLSGFLGAGKTTVLNHILRNREGLRVAVIVNDMS
                     EVNIDAQLVRQGEATLNRVEEKLVEMSNGCICCTLREDLLIEVAKLAREGRFDYLVIE
                     STGISEPLPVAETFTFADETGQSLSDVAQLDTMVTVVDALNFLKDYAEGESLLERGVA
                     LGEDDDRSIVNLLIDQIEFSDVILLNKTDLIAEDDLAMLEAILHHLNPEAKLVRTQFG
                     KAPLTEILNTGRFDFERASQAAGWLQELRGEHTPETLEYGISSFVFRARKPFHPQRLW
                     TFLTRRGKSEWWENIVRSKGFFWLASRPTELAVWSQAGHDLRIQPGGIWWADTPREEW
                     PEDPELHEEIRQLWDPEAGDRRQELVFIGIDLDQDLIEYELNTCLLTDSELAFDSEYW
                     ETIEDPFPQWEDEEAGELVDA"
     gene            1421..1702
                     /locus_tag="PKZ53_18585"
     CDS             1421..1702
                     /locus_tag="PKZ53_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42504.1"
                     /translation="MLGICDFSDEKVTSGCEREVGGRFSQLDGVGGSKPMSWLYSCSD
                     KYLRGVCVVHTLETIEESEFPFCVFRMFRGSLPEILYLIGPNAPSVSSS"
     gene            1744..>1926
                     /locus_tag="PKZ53_18590"
     CDS             1744..>1926
                     /locus_tag="PKZ53_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42505.1"
                     /translation="MKYAFRGAIFGDFFFRKVDLTAPQTEFKKSHSHGSVFPTIMQLQ
                     NLPISLFYAVTRNWRRS"
BASE COUNT          485 a          484 c          436 g          521 t
ORIGIN      
        1 ttagtggttt cccagtgctt gggtttggtc ccaaccaaca aaaaaaccca ccttcaacgt
       61 gaaaggtggg ttgctttatc gctgggagta gtagacggat aaaagtttga attcagttgg
      121 ctcaagcatc gacaagctcg ccggcttctt catcttccca ctgaggaaac ggatcttcga
      181 ttgtttccca gtattcagag tcaaaggcca gctcagaatc agtcagtaga caggtattga
      241 gttcatattc aatcaggtcc tgatccagat caattccaat aaacaccagt tcttgccgcc
      301 gatcaccagc ctctggatcc cataattgcc tgatctcttc gtggagttct ggatcttcag
      361 gccattcttc acgtggagtg tcagcccacc agatgccacc gggttgaatc cgcaaatcat
      421 gcccggcctg gctccacacc gccagttctg ttggacgcga agccagccag aaaaatcctt
      481 tggaacgaac aatattttcc caccattcgc tctttccacg cctggttaga aaagtccaaa
      541 gccgttgtgg atgaaagggt tttcgggcgc gaaaaacaaa gctgctgatt ccatattcga
      601 gtgtttcagg ggtgtgttca ccacgcaatt cctgaagcca gccagccgcc tgactggccc
      661 gttcaaaatc aaaccgtcca gtattgagaa tttccgttaa tggggctttt ccaaactgtg
      721 tgcgcaccag tttggcttct ggattgaggt gatgcaggat ggcttcaagc atggccagat
      781 cgtcttcagc aatcagatcc gtcttgttga gcaggatgac gtcactaaat tcgatttgat
      841 caatcaacaa attgacaatt gaccggtcgt catcttcgcc aagggccaca ccacgctcca
      901 gcaaactttc gccttcggca tagtccttca gaaaattcag cgcatcaact acggttacca
      961 tcgtgtctag ttgtgcgaca tcagacaggc tttgaccagt ttcatcggca aaagtgaagg
     1021 tttcggcaac aggtaatggc tcagaaattc cggtggactc aatcaccaga taatcaaacc
     1081 ggccttcgcg ggcgagtttg gcgacttcaa ttaagagatc ctcacgcaac gtgcagcaaa
     1141 tacaaccatt tgacatttca accagtttct cttcaacccg gttgagcgtg gcttcaccct
     1201 gacgcacaag ttgcgcatca atgttgacct cgctcatgtc atttacaatg actgccaccc
     1261 gcagtccttc acgattcctc aaaatgtgat tgagtaccgt ggttttccca gccccgagaa
     1321 agcctgacag cacagtcaca ggcaactttg gcttcgctga catgatattt gcctcccaat
     1381 tctgaatctc tttttcaggc gcgaaaaaag aacggtcagt gtgttgggaa tttgtgattt
     1441 cagcgatgaa aaagtaacca gcggctgtga aagggaggta ggtggcagat tttcgcaatt
     1501 ggatggagtt ggtggctcaa agccaatgag ctggctctat agctgttcag ataaatattt
     1561 gcgtggtgtt tgtgtagttc atactttaga aaccattgaa gaatctgaat ttccgttctg
     1621 tgtattccgt atgttccgtg gttctttacc tgaaattcta tatctcattg gacctaatgc
     1681 accttctgtg tcttcttctt gagaaagtcc atcatcacat actgcccgag attgcaggtc
     1741 gttgtgaaat acgcttttcg aggggcaata tttggtgatt tctttttccg gaaggttgac
     1801 ctcacagcgc cacaaaccga attcaaaaag agccacagcc acggctctgt tttcccaacg
     1861 attatgcagc ttcagaatct acccatatcc cttttttatg ccgtcaccag gaactggcgg
     1921 cgaagc
//
LOCUS       DAOQDO010001338         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60273_length_1925_cov_2.968449, whole genome shotgun sequence.
ACCESSION   DAOQDO010001338 DAOQDO010000000
VERSION     DAOQDO010001338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60273_length_1925_cov_2.968449"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="PKZ53_18595"
     CDS             <1..1623
                     /locus_tag="PKZ53_18595"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNJ42506.1"
                     /translation="PAAPAAPATATGGKPATVPAAASVAAAATPPPAETKPAEEKPAD
                     KSATDKPYLMQPEKTVTAQLDAVATAVAGMAAPTSKSLEVQFAPDPCTYDGRFANNGW
                     LQETPKPITKVTWENAVHLSPKTAGQKNLKSEDLVEISFGGRKVATAPVWIVPGHADD
                     SATLHLGYGRSRAGQVGNEAGFNVFALRSTTSLWGGDGLSLNPTGETHHLASTQLHFR
                     MEDRELIRVGTLEEFHKLLDGKNAEKPSFAKEGVDLEIEEENKKKKEPVTVFSESDHI
                     DNPNGYQWGMAVDTSSCTGCNACVVACTAENNIPVVGKLEVLRGREMHWMRIDQYHTG
                     SPDDPDIYVQPVMCQHCENAPCEVVCPVEATSHSTEGINEMTYNRCVGTKYCSNNCPY
                     KVRRFNFLAYSDYDTPVLKLGRNPNVTVRSRGVMEKCTFCVQRVNAARIEAEKRGTPV
                     ADGEIVTACQGACPAEAIVFGNINDPKSKVSELKEEPTNYALLAELNVKPRTSYLATV
                     RNVNKDIPKTADKPEWEHNSLSEESKTWLLPS"
     gene            1605..>1925
                     /locus_tag="PKZ53_18600"
     CDS             1605..>1925
                     /locus_tag="PKZ53_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNJ42507.1"
                     /translation="MAVAELDKQYTYDEKGEIVVVEPGHNFESVTGSITDVVLTPLEK
                     TPKGWIFGFFIAFSILQLLMVSVAWLLYRGVGIWGINVPIGWGFDIVNFVWWIGIGHA
                     GTLIS"
BASE COUNT          473 a          539 c          539 g          374 t
ORIGIN      
        1 cccgccgcgc cagccgcacc ggcgaccgcc actggcggga aacccgccac ggtgccagcc
       61 gccgcttcag tcgccgccgc ggcgacacct cctcccgccg agacaaaacc ggctgaagaa
      121 aagccggctg acaaatcagc caccgacaaa ccctatctga tgcagccgga aaaaacagtg
      181 accgcccagc ttgacgcggt ggccacggcg gtggctggga tggccgcccc aaccagtaaa
      241 tcgctggaag ttcaatttgc cccagacccc tgcacctatg atggccggtt tgccaacaac
      301 ggctggttgc aggaaacgcc caaaccaatt accaaggtga cctgggaaaa cgctgtccat
      361 ctgagtccaa agaccgccgg ccaaaagaat ctcaagagcg aagatctggt tgaaatcagt
      421 tttggtggtc gcaaggtggc cacggccccg gtctggattg ttcccggaca cgcggatgac
      481 tcagccacgc tccacctcgg gtatggccgc tcacgggcgg gacaggttgg gaatgaagcc
      541 gggtttaacg tgtttgccct gcgctcaacc acttcgctgt ggggcggtga tgggctgtcg
      601 ctcaacccaa ctggcgagac ccaccatctg gcctcaaccc agctccactt ccggatggaa
      661 gaccgtgaat tgattcgggt tgggacgctt gaagaattcc acaaactgct cgacggcaaa
      721 aatgctgaaa aaccatcctt tgccaaagag ggcgttgacc tcgaaatcga agaagagaac
      781 aagaagaaaa aagaaccggt gacggttttt agcgaaagcg atcacattga taatccaaat
      841 ggctatcaat ggggcatggc ggttgacacc agttcctgca ctggctgtaa cgcctgtgtc
      901 gtggcctgta ccgcggaaaa caacattccg gtggttggga agctggaagt gttgcgcggt
      961 cgcgaaatgc actggatgcg cattgaccag taccataccg gaagcccgga cgacccagat
     1021 atctacgtcc agcccgtgat gtgtcagcac tgtgaaaacg caccttgcga agttgtgtgc
     1081 ccggttgaag cgaccagcca cagcaccgaa ggcattaacg aaatgaccta taaccggtgc
     1141 gtcggtacca aatactgctc aaacaactgt ccgtacaagg tccgtcgctt taacttcctg
     1201 gcctactctg attatgacac cccggtgctc aaactgggcc gcaacccgaa cgtgaccgtc
     1261 cggagccgag gggtcatgga aaagtgcacg ttctgtgtgc agcgtgtgaa cgcggcccgg
     1321 atcgaagccg aaaagcgcgg aacccctgtt gccgacggcg agattgtgac tgcctgccag
     1381 ggcgcctgcc cggcggaggc gattgtgttt ggaaatatca acgatccaaa gagcaaagtc
     1441 tccgaattga aagaagaacc aaccaactac gccctgttgg ctgaactcaa cgtcaagcca
     1501 cgaacttcct acctggccac ggtgcgcaat gtcaacaaag acattccgaa gacggctgac
     1561 aaaccagagt gggaacacaa ttcattatct gaggagtcga agacatggct gttgccgagt
     1621 tagacaagca atacacatat gatgaaaagg gtgagattgt cgtcgtcgag ccggggcata
     1681 attttgaatc agtcaccgga tccattaccg acgtcgttct gaccccactc gaaaaaaccc
     1741 cgaaagggtg gattttcggg ttctttattg cattttcgat tctccagctt ctgatggttt
     1801 cggtggcctg gttgctctac cggggggttg gtatctgggg gatcaacgta cccatcggct
     1861 ggggctttga cattgtcaac tttgtgtggt ggatcggtat cggccacgcc ggaacgttga
     1921 tctcg
//
LOCUS       DAOQDO010001339         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60402_length_1923_cov_2.378480, whole genome shotgun sequence.
ACCESSION   DAOQDO010001339 DAOQDO010000000
VERSION     DAOQDO010001339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60402_length_1923_cov_2.378480"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /gene="tssK"
                     /locus_tag="PKZ53_18605"
     CDS             complement(<1..245)
                     /gene="tssK"
                     /locus_tag="PKZ53_18605"
                     /inference="COORDINATES: protein motif:HMM:NF017723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNJ42508.1"
                     /translation="MKDAIAGTEGRAPLEAARQAFSIRHESDGLAGFVTLGVARIVSR
                     EADGGVVLDPEYFPPCLRFDVHPLLASIRASGVPVRAV"
     gene            275..577
                     /locus_tag="PKZ53_18610"
                     /pseudo
     CDS             275..577
                     /locus_tag="PKZ53_18610"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            818..1771
                     /locus_tag="PKZ53_18615"
     CDS             818..1771
                     /locus_tag="PKZ53_18615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42509.1"
                     /translation="MIDSKELIEFPDRLLTPKHQILPTPEHNQSPLVSIIVRTKNRPF
                     LLRQALASVAAQTYANLECVIINDGGIDVRDIVDSIRGKLSIQYYASDQPVGRTAAAN
                     RGLQVASGDYFNFLDDDDVLYPHHVEKLVTYLHTTGRHFAYSDCVTACYRLTPDGLDL
                     TSAKPPVLLSQDFDRSRLYVSNFIPIMTAMFSRKLYETVGPMDETFPMFEDWDYWIRM
                     AEIVDLQRIPGVSAQYRFFSDPKADTSEWTARMYAKHMSSWFSQRVIPNLLEQIEDLK
                     ATNLMLQQQLKTRSLRSSARKIITDKTRSIVNWLRQVKPTR"
BASE COUNT          464 a          505 c          514 g          440 t
ORIGIN      
        1 acggcgcgca cggggacgcc gctcgcccgg atcgaggcga ggagcggatg cacgtcgaag
       61 cgcaggcagg gcgggaagta ttcgggatcg aggaccacgc ctccgtcggc ttcgcgcgag
      121 acgatccggg ccacgcccag cgtcacgaag ccggccaggc cgtcgctttc atgacggatg
      181 ctgaacgcct ggcgtgcggc ctcgagcggt gcgcgcccct ccgtgccggc tatggcgtcc
      241 ttcacctcga tgatgcgacc gcggtggcgc gctccggttg catacgcacc atcgggaccc
      301 ggtcaaactg gtcgttttaa cggatggaag agctgccaat caaagcggaa gcgaagtatc
      361 ccgttatatc aaaaagcgtg agacggaaac ccggcaagca gctcgaatcc ttgggattac
      421 agatattgaa ttttgggaaa tcccggatcg gacattgctc catcattcag acgcaatcgt
      481 gaaactcagt gttctcttgt ccagttttgg accgactttg atttatgccc cgtcattgct
      541 agagtttcac cctgatcacc gggcgacagc cgaattgctg attcaggcgg ttatcgagtc
      601 caggcaccag gccaacgtgg cgttttatga gtacaacacg ccgctccagg tcaattgcct
      661 ggtggatatt tcgtcagtgg ttgagacgaa gcgggccgcc tgtgaagtct atcgcaccca
      721 gcatgagaat tttgcctatg cggactttgc tttgagttta aatcgctatc gagcgttgac
      781 tatctcgcat acctgccagg ctgccgaagg gtttttaatg attgactcaa aagaactgat
      841 tgagtttcct gaccggctct taacccctaa acaccagatt ctccccacac cggagcacaa
      901 ccagtcaccg ctggtctcaa ttattgtgcg aacgaaaaat cgcccgtttc tcctgcgcca
      961 ggccctggcc agtgtggctg cccaaaccta tgccaatctg gaatgtgtga tcatcaatga
     1021 tggtggaata gatgttcggg acattgttga ttccattcgt ggaaaacttt cgattcagta
     1081 ttatgcctct gaccagcctg ttggtcgcac ggcagccgcc aaccgtgggc tacaagtcgc
     1141 cagcggagac tatttcaatt ttcttgatga tgatgacgtg ttgtatccac accatgtcga
     1201 gaaactcgtg acctatctgc acacaaccgg caggcatttt gcctatagtg attgtgtaac
     1261 ggcctgctat cgcctgacac ctgacggatt ggatttgacc agtgccaaac cgccggttct
     1321 cctctctcag gattttgacc gaagccgcct gtatgtaagc aatttcattc cgatcatgac
     1381 tgcgatgttt agccgaaaac tgtatgagac ggttgggccg atggatgaaa cttttcccat
     1441 gtttgaagac tgggattact ggatacgaat ggcagaaatt gttgatttac aaaggattcc
     1501 gggtgtttca gcgcagtatc ggttcttttc tgacccaaaa gctgatacca gcgaatggac
     1561 ggccagaatg tatgccaaac atatgtcgtc ctggttttcg caacgggtca ttcccaacct
     1621 gctggaacaa atcgaagacc tcaaagccac aaatctgatg cttcagcaac aactcaaaac
     1681 gcgaagcctt cggtcatcag ccaggaaaat aattaccgac aaaacccggt caattgtgaa
     1741 ttggcttcgg caggtgaaac cgacaaggta aggattgggt tgagggtttt cgaccgcagg
     1801 cctgaaaccc ttattctggc gcttatgggg gcaggggtca gggagcgggg aaaagactaa
     1861 aaggacataa aggacataca ttcgaaaacc cggaacccgg aacccggaac ccggaacccg
     1921 gaa
//
LOCUS       DAOQDO010001340         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60456_length_1922_cov_2.906802, whole genome shotgun sequence.
ACCESSION   DAOQDO010001340 DAOQDO010000000
VERSION     DAOQDO010001340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60456_length_1922_cov_2.906802"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1922)
                     /locus_tag="PKZ53_18620"
     CDS             complement(<1..>1922)
                     /locus_tag="PKZ53_18620"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42510.1"
                     /translation="DRANGDFFGTSVAIEGDTAVVGARLGPGGTSADQGAAYVFVRSS
                     GVWSQQARLNGSDSAANDEFGRSVSMSGQTVVVGAPLGPGTNSADQGAAYVFTRSVTT
                     WTQQQKVSAGSDGAAGDQFGLAVDVNLNTAIVGANSDDFPGQANQGSAYVFFRTGTTW
                     AQQQKLSGSDSLANDNFGVSVALSNETAIIGADRDDIGANTDQGSAFVFVRSGSTWSQ
                     QQKLTGSDGTANNQFGAGVDIDGERAVIGARFGRNAVPVASGSVYIFGRAENLWFQQK
                     ILVANDGAANDEFAGCVALDDNYIACGARADDVGANADQGSSYVFVLECPTCPYLPAV
                     LTGTRTICVGQSAPITVYIEGGVGPFSNVTLSNGGGTQSGSGPFTFTVSPTSTTAYSI
                     IGGTDGNGCPISGLGSVLITFGSSPTAADAGLDQTICITGTAQLNGNNPAPQTGVWSV
                     ASGPSTANSQFNNVNLRNAIFDPDLAGTYVLQWSIPSPPCSPSTDTVTILVRPTPTTA
                     NAGSPQTICSNSSATLNGNALGVGTGMWSVVSGPSTDNNQLLLPANRNAIFTPSGGAG
                     SYVLRWTISNPPCADSTSDVTITVDPAATVDAGANQTVCDTNPTVTLAGVIGGSATLA
                     TWSGGGGTFTPNTTTL"
BASE COUNT          450 a          545 c          515 g          412 t
ORIGIN      
        1 cagcgtggtg gtgtttgggg taaacgttcc gccacctccg ctccatgtgg ccaacgtggc
       61 gcttccgcca ataactccag ccagagtaac agttgggttg gtatcgcaca ctgtttgatt
      121 tgccccggca tcaaccgtcg ctgccggatc aaccgtgatg gtgacatctg aggtcgaatc
      181 agcacagggc gggtttgaaa tcgtccatct gagcacataa gaaccagcgc ctccgctcgg
      241 ggtaaagatg gcgtttcgat tggctggaag caaaagctgg ttgttgtcag ttgaagggcc
      301 actcacaacg ctccacattc cagttccgac gcctagcgca tttccattta aagtcgctga
      361 actatttgaa caaatcgtct gtgggctccc agcattggcg gtggtcggtg ttggtcgaac
      421 cagaattgtc acggtgtcgg tcgaagggct acacggcgga ctcggaattg accattgcag
      481 aacatacgtt ccagccagat ccggatcgaa aatcgcattc cgcaaattca cgttgttgaa
      541 ttgcgaattc gccgtcgaag gcccgctcgc aaccgaccac acgcccgttt gcggggccgg
      601 attgtttcca ttcagttgtg ccgtgcccgt aatacaaatt gtttgatcca gcccggcatc
      661 agccgcagtc gggctggaac caaatgtaat caggacactt ccaagaccgg aaatcggaca
      721 gccgttgcca tctgttccac caataattga ataggctgtg gtcgaagtcg gagaaacggt
      781 aaaggtaaac ggtccgctcc ctgactgggt accgcctccg ttgctcaaag taacatttga
      841 aaatggcccg acgccgcctt caatataaac cgtgatgggt gcggattggc cgacacaaat
      901 ggttcgggtt ccggtcaaaa cggcgggaag gtagggacag gttgggcact ccaaaacaaa
      961 aacataagaa gagccttgat ctgcattggc ccccacgtca tctgcccggg cgccgcacgc
     1021 gatgtagttg tcatctaatg ccacacatcc ggcaaattca tcattggccg cgccgtcatt
     1081 ggcaaccagt attttctgct gaaaccacag attttccgct cgaccaaaga tgtaaaccga
     1141 tccgctggca actggaaccg cattacggcc aaatcgagcg ccaatcacgg cgcgttcgcc
     1201 atcaatgtca actcctgctc caaactggtt gtttgcggtt ccgtcgctgc cagtcaattt
     1261 ttgctgctgg gaccaggtgg agccagaccg aacaaacaca aacgccgaac cctgatcggt
     1321 attggcacca atatcgtctc tgtcggcgcc aataatcgca gtttcattac tgagagcgac
     1381 cgaaacacca aagttgtcgt tggccagact atcactgccc gaaagtttct gttgttgagc
     1441 ccaggtcgtt ccggttcgga agaaaacata ggctgaaccc tgattggcct gacctgggaa
     1501 gtcgtctgaa ttggcaccga caatggctgt gttgagattg acatcaacag ccaggccaaa
     1561 ctgatcgcca gcggcgccgt cggaaccggc agacactttc tgctgctgcg tccaggtggt
     1621 gaccgacctg gtaaaaacat aggcggcgcc ctggtcggca ctattggtgc ctggtcccag
     1681 tggggcgcca accacaacgg tctgaccgct cattgagact gaccggccaa attcatcatt
     1741 ggcggcactg tcactgccgt ttaagcgggc ctgttgcgac cagacaccag aggaccggac
     1801 aaaaacgtaa gcggccccct ggtcagcact cgttccacct gggccaaggc gggccccaac
     1861 cacggcggtg tcgccctcaa tggcaactga tgttccaaaa aagtctccgt tggcacggtc
     1921 gc
//
LOCUS       DAOQDO010001341         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60504_length_1921_cov_3.155413, whole genome shotgun sequence.
ACCESSION   DAOQDO010001341 DAOQDO010000000
VERSION     DAOQDO010001341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60504_length_1921_cov_3.155413"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PKZ53_18625"
     CDS             complement(<1..127)
                     /locus_tag="PKZ53_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNJ42511.1"
                     /translation="MRVLVIEDEVKMASLVERGLKEEGMAVDVVHDGEDGFIQALQ"
     gene            complement(187..537)
                     /locus_tag="PKZ53_18630"
     CDS             complement(187..537)
                     /locus_tag="PKZ53_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNJ42512.1"
                     /translation="MADPNNIFLKIARREIPADIVFEDEQCVAFRDINPQAPVHILVI
                     PRKPLESISEATTEDKELLGHLLLVAGQIAQTTGIAQTGYRVVVNTGRNAGQTVFQLH
                     VHVLGGRDLGWPPG"
     gene            complement(685..>1921)
                     /gene="murA"
                     /locus_tag="PKZ53_18635"
     CDS             complement(685..>1921)
                     /gene="murA"
                     /locus_tag="PKZ53_18635"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNJ42513.1"
                     /translation="KKPLEGRISVSGAKNAALPCMAAALLTSDAVTLHNLPYVRDIIT
                     MRRLIEDMGGLVLMPERGTLKIQADPIQFCEAPYELVKTMRASVLVLGPLVARFGHAS
                     VSLPGGCAIGQRPIDLHLTALTKMGATIELVNGNVVAHADKLVGAEIYFDQVTVTGTE
                     NLMMAGALASGRTILQNAASEPEVVDLAVLLNRMGARITGAGTPTVVIEGVERLRGAE
                     HTIIPDRIEAGTLLAAAAITQGHLEITDCNPDHLTAVIDKFQEAGLRVNRLSDVALHA
                     SYAGPLKASNVTTLPFPAFPTDMQAQYLAVMTHAEGTSVITETIFENRFMHASEMMRM
                     GAHITLRGNQAIVSGTGKLTGAGVQASDLRASASLVLAGLAADGETIVDRVYHIDRGY
                     ERIEEKLCSVGAQIERFRN"
BASE COUNT          448 a          511 c          515 g          447 t
ORIGIN      
        1 attgaagtgc ctggatgaaa ccatcttcgc catcatgaac cacgtcaacg gccattcctt
       61 cttctttcag accgcgttcc accagcgaag ccattttgac ttcgtcttcg atcaccaaaa
      121 ctctcatgtc agtataatta cccgttgatg tgactggatt gccttctgta agaagatacc
      181 tgacttttat cctgggggcc acccaaggtc acgcccacca agcacatgga catgcaattg
      241 aaagacggtt tggccagcgt tgcgcccggt attgacaacc acacggtatc cggtttgagc
      301 aatgccggtt gtctgagcaa tctgacctgc gaccagcaac aagtgcccca gcaattcctt
      361 gtcttcggtg gttgcctcgc taattgactc aagtggtttg cggggaatga ccagaatatg
      421 aaccggagcc tgggggttga tatcgcgaaa agcgacacat tgctcatctt caaacacaat
      481 atctgccggg atttcccgac gcgcaatttt caggaagatg ttgtttggat ctgccataaa
      541 agggaagtcc ttatgaagga aagccgtaac cagtagcgtg acgtcgtgac atagcgaagt
      601 agaaaaaaca cacgagcaga gttgagctat tgacagcaac atcgccgaaa tatttatctg
      661 aaaagctata gttcgctctg cttgttaatt ccgaaaccgt tcgatttggg cgccgaccga
      721 gcacaatttt tcttcgattc gttcatagcc ccgatcaatg tgatagaccc ggtccacaat
      781 ggtttcgccg tcggcagcca gaccagccag gaccagggaa gcactggcgc gcaaatcaga
      841 cgcctggact ccggcgccag tgagtttccc ggtgccgctg acaatggcct gattcccacg
      901 caaggtgata tgggcgccca tgcgcatcat ttcactggcg tgcataaacc ggttttcaaa
      961 aatcgtttca gtgatgaccg aggtcccttc agcgtgagtc atcaccgcca ggtattgagc
     1021 ctgcatatca gtggggaagg ctggaaaggg aagtgtcgtc acgtttgagg ctttcagtgg
     1081 gccggcgtag gaggcatgga gggcgacatc gctcaagcga ttcacccgaa ggccggcttc
     1141 ctgaaacttg tcaattaccg ccgtcaggtg atccgggttg caatcagtga tttcaagatg
     1201 gccctgggta atggcggcgg cagccaggag ggttccggct tcaatccgat ctggaatgat
     1261 cgtgtgctca gcgcctcgca atcgctcgac gccttcaatg acaacggtcg gtgttccggc
     1321 acctgtgatt cgagcgccca ttcgattgag caggacagcc agatccacga cttcaggttc
     1381 gctggcagcg ttttgcagga tcgtccggcc actggccagc gctccggcca tcatcaggtt
     1441 ttcagttccg gtgacggtta cctggtcaaa atagatctca gctccaacca gtttgtcggc
     1501 gtgggcgacg acattgccat ttaccagttc aattgtggca cccatttttg tcagggccgt
     1561 cagatgaagg tcaattggtc gctgaccaat ggcacacccg cccggcaaac tgacactggc
     1621 atgtccaaac cgggcaacca gcggtcctaa aaccagaact gaagcccgca tggttttgac
     1681 cagttcatag ggggcttcgc aaaattgaat tggatcagcc tggatcttta acgtgccgcg
     1741 ttccggcatc agaaccagac cgcccatgtc ctcaatcagt cgccgcatgg tgatgatgtc
     1801 ccggacatag ggaagattgt gtaatgtgac cgcatctgag gtgaggaggg cggctgccat
     1861 acaggggagg gctgcatttt tggcaccact gacactgatg cgcccttcga ggggtttttt
     1921 a
//
LOCUS       DAOQDO010001342         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60513_length_1921_cov_2.747588, whole genome shotgun sequence.
ACCESSION   DAOQDO010001342 DAOQDO010000000
VERSION     DAOQDO010001342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60513_length_1921_cov_2.747588"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..1059)
                     /locus_tag="PKZ53_18640"
     CDS             complement(418..1059)
                     /locus_tag="PKZ53_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42514.1"
                     /translation="MPTRWKCLSGKKISSLTLGVSLLWFVGCNTPAPSPQSPSSSSLP
                     APAPVQPAKPEPSSQEPLAPLPNKNRKLVKTYASAEELTKAFLKVLADKPKAKDLLTF
                     AVTKDEYCQYLVPELPGGKIPNVTCDFLWNQDQIPSSYGLQSTLSKYSGKKYTFLNIS
                     FTKKPETYETYRVYRFPRVKVLTETNTEEVIAPFGPVVEIDGKFKLYGFGKDD"
     gene            complement(1236..>1921)
                     /locus_tag="PKZ53_18645"
     CDS             complement(1236..>1921)
                     /locus_tag="PKZ53_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42515.1"
                     /translation="TVNVKHLLSLGNGDDAFDWVNGWVGKCQFLIALRDGTTEAQNGV
                     EADNLNESNDVLPRSAPTIYNMTLIGDLETKSSSAFGMLLRVGTAGTFRNIIVKNFRE
                     FGIRVQNDSTLTQLQNGALSIDGLIIHGNGKGAFEFVANSGSPNPGDLSNLFRNVNTE
                     DPKLTNISKTATPADFRPQTGSPALNQALVAARPQGDTFFEAANFLGGMGPNATDDWT
                     KGWTTFVRK"
BASE COUNT          479 a          421 c          481 g          540 t
ORIGIN      
        1 gtaataacta ttttcccttt agaatcaaca acttgaaaat tttacaattg agaaaattgc
       61 tcattttttc ttgtcgaaaa tttctaaacc attgattcta aagcaaaaat aatttggccg
      121 ggcataagtc tacctcctga ttgaaaaagt gatttttcaa cctgtgctgc ttaccgcgga
      181 ttaaatcggt gggagtcaaa atggctttga tttaggggcg ggaaatcaag agcaactact
      241 gttaaaactg gttgggatca gttgtgtttc catcaaaaaa tacaggtgtt cgtcttgaat
      301 caaagcctag cacaattgat atgaatgcag gatgaatggt caggtgtaga ggtggatctg
      361 ccgcaggtgg tatctgggca ccgtccttgc gtccggcgcc cttttcaaaa tcctgggtta
      421 gtcatctttc ccaaacccat acaatttgaa ttttccatca atttcaacca ccgggccaaa
      481 cggggcaatg acttcctcgg tgttggtttc agtcagtacc ttcacccgtg gaaaccgata
      541 aactcgatat gtctcatagg tttctggctt cttggtaaaa ctgatgttga gaaaggtgta
      601 ctttttcccg ctatattttg aaagcgtaga ttgcagacca tagctgctgg gaatttgatc
      661 ctgattccac aaaaagtcac aggtgacatt ggggattttc ccgccaggaa gctccggcac
      721 caaatactgg caatattcat ctttggtaac agcaaaggtg agcaggtctt tcgcttttgg
      781 tttgtcagcc agcaccttca aaaaggcttt ggtcaactct tcggctgagg cgtaggtttt
      841 caccagtttt cggtttttgt ttggcaatgg ggcaagcggt tcttgactcg atggttctgg
      901 tttggctggt tgaactggtg ctggagcggg aagggaactg gaagaagggg attgtggtga
      961 tggcgcagga gtattgcatc cgacaaacca cagaaggctc acgccaagtg tcaaactcga
     1021 aatcttctta ccactcaagc atttccaacg tgtaggcatg gtctttctca ttctggttga
     1081 aacaccaacg agccatgaaa actcatggct cgttgggtga ttgtctattt tgctacatca
     1141 tcaagtctac caatttgcaa tggaatcctt ggatgaggaa gcagttaagt agtttaatta
     1201 aatagactta gcgaactact gactcctgac tgatactatt ttcgaacaaa ggtggtccag
     1261 cctttggtcc agtcatcggt tgcatttggc cccatgccgc ccaggaagtt ggccgcttca
     1321 aagaaggtgt caccctgtgg gcgagccgca accagcgcct gattgagggc tggtgaaccg
     1381 gtctgtggcc gaaaatcagc cggggtggcg gtcttgctga tgttggtcaa ctttggatcc
     1441 tcagtgttca cgttgcggaa gagatttgac aggtcgcctg ggtttggtga accagagttg
     1501 gcgacaaatt caaaggcacc tttgccattg ccgtgaatga tcaatccatc aatgctcaat
     1561 gctccattct gcaattgtgt cagggttgag tcattctgga cacggattcc gaattcacgg
     1621 aagttcttga cgatgatgtt gcggaaggtt ccagccgttc caacccgcag gagcattcca
     1681 aaagctgagc ttgacttggt ttccaggtca ccgatgagcg tcatgttgta gattgttggg
     1741 gctgaccgtg gcaacacgtc attgctttca ttgaggttat cagcttcaac gccgttttga
     1801 gcttcagtgg taccatcacg aagcgcaatc aggaactgac atttgccaac ccagccgttg
     1861 acccagtcaa aggcatcatc gccattgccc agcgaaagca gatgtttgac gttgacggtg
     1921 c
//
LOCUS       DAOQDO010001343         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60515_length_1921_cov_2.645766, whole genome shotgun sequence.
ACCESSION   DAOQDO010001343 DAOQDO010000000
VERSION     DAOQDO010001343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60515_length_1921_cov_2.645766"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKZ53_18650"
     CDS             complement(<1..306)
                     /locus_tag="PKZ53_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42516.1"
                     /translation="MKMLHTLRPGTKALALMLGIIWVGALLTLPSTLTFHLPPVMAAS
                     PHQELQLPNGFTQTLVASNFGGFPTCMAIAPDGRIFICLQNGTMRLVKNGSLLPDPVI
                     "
     gene            742..1059
                     /gene="groES"
                     /locus_tag="PKZ53_18655"
     CDS             742..1059
                     /gene="groES"
                     /locus_tag="PKZ53_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNJ42517.1"
                     /translation="MSSKIRPLYDRIIVKRREEGEQIRGGIIIPDTAKEKPQEGDVIA
                     VGGGKVREDGTRIPLDVKAGDRVLFGKYSGSEIKVDGEEFLIMREDEILGVIEGATLG
                     QSA"
     gene            1172..>1921
                     /gene="groEL"
                     /locus_tag="PKZ53_18660"
     CDS             1172..>1921
                     /gene="groEL"
                     /locus_tag="PKZ53_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNJ42518.1"
                     /translation="MAKLVIHGEESRQAILRGVNQLADAVKVTLGPKGRNVVIEKKFG
                     SPTITKDGVTVAKEIDLKDNMENAGAQMVREVASKTSDVAGDGTTTATVLAQAIFREG
                     AKLVAAGHNPMSLKRGIERAVEVAVAKIKDMAKPVVKGDAIAQVGTISANGDKLIGEI
                     IASAMEKVGKDGVITVEESKSLETTLEVVEGMQFDRGYLSPYFVTDPERMEASLENAM
                     LLLHEKKISSMKDLLPVLEQVARQGKPLVIIA"
BASE COUNT          514 a          440 c          546 g          421 t
ORIGIN      
        1 aataacaggg tctggaagca aactcccatt tttgaccagg cgcatagttc cattctgaag
       61 acagataaag attcggccat ccggggcaat ggccatacag gttggaaatc ccccaaagtt
      121 actggcaact aaagtttgtg taaatccatt tgggagttga agctcctgat gaggtgaagc
      181 cgccatcacc ggcggcagat gaaaggttag agtggatgga agtgtcagca gagcgccgac
      241 ccatatgatc cccagcatca gtgccagggc tttggtgcca gggcgtagcg tgtgaagcat
      301 cttcatagtg tgaacccttt caagaagtta cctggatgtg gctgtgggca agcgaagtgt
      361 cgtcaacaaa tcgtttccag gtaggtgaaa agccgggccg tcatcaacgg cgggaaggct
      421 gtaaggtctt ctctcaaagt gtgaatgccc aatcttacac aattatttag cggaaaggga
      481 actggttttg ttttcgggcc acgaaaaata gtgcgaataa gaaagttctg accagagaga
      541 gtgtgtggct gcgaaggtgt gtttgactgt cagggcggga accctggaac tggggactct
      601 accctggttg aagtcggaaa agtcagccgg ggaacctctt gacaaggctg gggctctttg
      661 tgtattttag cagtcaccta ctgagagtgc taacactcat acgctaaatt caatgaaatc
      721 aaggagattg cattaacgcc tatgtcaagc aagattcgtc cgctctatga tcgcatcatt
      781 gtcaaacgcc gtgaagaagg cgaacaaatt cgcggtggga tcattatccc ggacacagcg
      841 aaagaaaaac cccaggaagg cgatgtgatc gccgttggcg ggggcaaagt cagagaagac
      901 ggcacccgta tcccgttgga tgtgaaagct ggcgaccggg tgttgttcgg caagtactcc
      961 ggatcagaaa tcaaagttga tggcgaagag ttcctcatca tgcgcgaaga tgaaatcctg
     1021 ggcgtgatcg aaggggccac gctgggtcaa tccgcttagt ttgcctgttg caaccactcc
     1081 aggctggcct ccgtgctggt ctgacgggtt ttcaggttgt ttatttcttc ataaatactt
     1141 atttgtctta tcttaaaagg agcaagttac aatggccaag ctagtgattc acggagaaga
     1201 gtcacgtcag gcaattctgc gtggcgtcaa ccaactcgcc gatgctgtaa aagtgaccct
     1261 cggtccaaag ggccgcaatg ttgtcattga aaagaaattt ggcagcccaa ccatcaccaa
     1321 agatggtgtg accgtggcga aagaaattga cctcaaagac aatatggaaa acgccggcgc
     1381 ccagatggtg cgcgaagtgg cgtcaaagac ttcagatgtt gccggtgatg gcaccaccac
     1441 cgcgacggtg ctcgcgcagg caatcttccg cgaaggcgcc aaactggtgg cggctggcca
     1501 caacccaatg tccctcaagc gtgggattga acgggcggtc gaagtggccg tggccaaaat
     1561 caaagacatg gccaagcccg tggtcaaagg tgatgcaatt gctcaggtcg gaacgatttc
     1621 agccaacggc gacaagctga ttggggaaat cattgcttcc gccatggaaa aagtgggcaa
     1681 agatggtgtc atcacggttg aagaatcaaa atcactcgaa accaccctcg aagtggttga
     1741 agggatgcag tttgatcgcg gctatctgtc accatacttt gtgacggatc cggaacggat
     1801 ggaagcgtca ctggaaaacg cgatgctttt gctccatgaa aagaaaatca gctccatgaa
     1861 agatctgctc ccagtcctgg agcaggtggc tcgtcagggc aaaccgctgg tgattatcgc
     1921 t
//
LOCUS       DAOQDO010001344         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60594_length_1919_cov_7.005901, whole genome shotgun sequence.
ACCESSION   DAOQDO010001344 DAOQDO010000000
VERSION     DAOQDO010001344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60594_length_1919_cov_7.005901"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKZ53_18665"
     CDS             <1..194
                     /locus_tag="PKZ53_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42519.1"
                     /translation="GFYCLYSIEEEELRLVSFSAGYNGLERIKIKYGKGKEFLGQKPA
                     RTHQGVNSSQPIIREQQSL"
     gene            289..603
                     /locus_tag="PKZ53_18670"
     CDS             289..603
                     /locus_tag="PKZ53_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42520.1"
                     /translation="MPDTHPYDSCWRMGDEEFHTQVFDFWWKNLNFDESQRIEYFCNW
                     PPPPCWLAWMIDVIWDPCPDECQEEFDYSTYFGQIERLGFGTQLQYQADLEDPRWSEN
                     SE"
     gene            complement(875..1756)
                     /locus_tag="PKZ53_18675"
                     /pseudo
     CDS             complement(875..1756)
                     /locus_tag="PKZ53_18675"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          472 a          551 c          404 g          492 t
ORIGIN      
        1 aaggatttta ttgcctttat tcaatcgaag aggaagaact ccgactggta agctttagcg
       61 ccgggtacaa tggtctcgaa cgaatcaaaa tcaaatatgg caaggggaaa gaatttcttg
      121 gccaaaaacc agcgcgaacc caccagggcg tgaactcaag ccagccaatt attcgagagc
      181 aacaatcttt atgaacaccc caaccagttc agaatcggac actcacatcc ttaaagaaat
      241 tgaacgactg gtcagccagt atggccagtt cctctgccct gggttgtgtt gcctgatact
      301 cacccgtatg acagttgctg gcgaatgggt gacgaagaat ttcataccca agtctttgat
      361 ttctggtgga agaatctgaa ttttgacgag tcccaacgca ttgagtactt ctgcaactgg
      421 ccgcctccac cctgctggct ggcctggatg attgacgtca tctgggaccc ttgccctgac
      481 gaatgccagg aggagtttga ttattcgacc tattttggac agattgaacg tcttgggttt
      541 ggcactcagt tgcaatatca agctgatctg gaagacccac gctggtctga aaattctgaa
      601 tagcaggcca gggcaccttt ctcctcacat aaaactcaat tcaaacttca atgactgaca
      661 gggttcttca aacctggtaa aaactggttg cgtgttcaaa gctgaggcaa cttcatttgt
      721 attcttcttt atgcgaggct ttgtattgag gaatcattaa aaaaacagct tcccgacttt
      781 cattttgggt cgaaaccacg ttcgtttgag ttttgacggc acttaaaagc taaagggctc
      841 cccattgtcc agacaaaaaa accaggtttt gaagttaatc cctgaggacg cgatgtgaaa
      901 gtgaatcggt caactcgcct gagttgaatt tctccttaga agacaaaaaa agcgctgccg
      961 gtggctgata ctcaagggcc tggtgaggcc gccgatagtt gtaaaactca aagtagttgc
     1021 ccaacccaag ccgggtctca accggcgttt catacccctg aaggtaaatg tcttcatatt
     1081 tgacagttcg ccacaaccgt tcgacaaaga tattgtccaa cgcccgcccc cgaccatcca
     1141 tgctgatgcg aatgccagct tcgaccagcc gaccagtaaa gtcgcggctg gtgaactgca
     1201 ccccctggtc ggtgttgaag atgtctggac ttccagactc caatgcccga tccagcgctc
     1261 gcaggcaaaa ctccgtatcc agtgtggttg acaactccca actgatcaca aagcgtgaat
     1321 accaatccat caccgccacc agatacacaa acccccggtt taatcggata taagtaatgt
     1381 cggtactcca cacctggttc actctctcca cctccactcc ccgcaacaga tacgggtaga
     1441 tttgatgccc gatcccggct tcactcagcc tcggtttcgg atagatggcc tccaacccca
     1501 tcacccgcat caaccgccgc acccgtttcg ggttcacttc ataccctttc gcccgcagca
     1561 ctaccgtcat ccgtctgact ccataatatg gatgcgccgt atacacctcg tccaattccc
     1621 gcatcacctc cagattttcc tcgctttccc ccactggctg gtaataccag cttgatcgcg
     1681 ccagtcccac caactcgcat tgccgttcga tgcttaactc cggatgtccc ggctcgatca
     1741 gctttcgctt ctcctcaact gagagctccc gctttttttt tcaaccactc cagctccatc
     1801 tgcagtttcc cgatctgttc gtacaatgct ccctgcagac tttcatccgc tttcagttgt
     1861 tttcgctccc ttttcccaaa tacttggggg agttcctcca ccgccatttt tttccattg
//
LOCUS       DAOQDO010001345         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60604_length_1919_cov_3.841202, whole genome shotgun sequence.
ACCESSION   DAOQDO010001345 DAOQDO010000000
VERSION     DAOQDO010001345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60604_length_1919_cov_3.841202"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKZ53_18680"
     CDS             <1..634
                     /locus_tag="PKZ53_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ42521.1"
                     /translation="DDTAEVARSLGIKTIVHTTNLGYGGNQKTCYREALKAGADIVIM
                     LHPDYQYTPKLLPAMAYLIACGHYDCVLGSRIIGKGALRGNMPAYKYFFNRCLTAFQN
                     ILLSERLSEYHTGYRAFSRQLLETLPLGENDNDFIFDNEMLAQIVYFDFQIGEISCPT
                     RYEADSSSINFIRSCKYGLGVLGVSMAFKLQKWGLGKWRIFDPNGKRLSE"
     gene            705..1121
                     /locus_tag="PKZ53_18685"
     CDS             705..1121
                     /locus_tag="PKZ53_18685"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNJ42522.1"
                     /translation="MNTSGETSLTQLVQAMQPSLADECFIFCTLAESIAIPLIPQTLG
                     FFREVEGVTLILEKEVALAHQIQFHGEWAKITLRVHSSLEAVGFLATISQPLAVAGIS
                     TNVISAFYHDHLFVQWDRREKAIEILNHLAQTAAHL"
     gene            complement(1112..1735)
                     /locus_tag="PKZ53_18690"
     CDS             complement(1112..1735)
                     /locus_tag="PKZ53_18690"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNJ42523.1"
                     /translation="MPSTLFPDVSGFILAGGESRRMGVNKARLLVEGVPMIERVLRTL
                     SAVTDDVSIVTNAPETYLRLGVPLLGDLRRDANRGAGPLAGIEAALNATTRSFSLIVA
                     CDLPFLTAEFLSLLLNYRQEAQIVVPETTGVQKPRLHSMCAVYQASVYPVVTRLLDQG
                     ERRMETLLEHTTVRRIRASEYADFPHSNRLLINVNTPDDFQAIQRHK"
BASE COUNT          495 a          455 c          466 g          503 t
ORIGIN      
        1 cgatgacact gctgaagttg cacggagtct tggaattaaa actattgttc acactacaaa
       61 ccttggctac ggcggtaatc agaaaacctg ttatcgcgaa gccctcaaag ctggtgctga
      121 tattgtcatc atgttgcacc cggactatca gtacacacca aagctgctcc cagccatggc
      181 ctacctgatt gcgtgcggtc actatgattg tgtccttggt tcacgcatca ttgggaaagg
      241 tgctttgcgt ggcaatatgc ccgcgtataa atacttcttc aaccgatgtt taaccgcctt
      301 ccagaatatt ttgctgagtg agcggttgtc tgagtaccac accggatacc gggcattttc
      361 acggcaattg ctcgaaacat taccgttagg cgaaaacgac aacgacttta ttttcgacaa
      421 tgaaatgctg gctcagattg tttactttga ttttcagatt ggtgaaatta gctgtccgac
      481 ccgctatgaa gctgattcct catcaatcaa tttcattcga agttgtaaat atgggctggg
      541 cgtgttgggc gtttcaatgg cattcaagct tcaaaaatgg ggattgggca aatggcggat
      601 ttttgatcca aatgggaagc ggttgagtga atagtgaaat agcgaagagt cagtactacc
      661 tgcgtcagcg ggtgggtgaa aaacagccgt tgggtaacca cactatgaac acttcgggtg
      721 aaaccagcct cactcaactg gttcaagcta tgcagccgag cctggccgat gagtgtttta
      781 ttttctgcac gctggctgaa tctattgcga ttcctttgat tccacagact ttaggattct
      841 ttcgtgaagt cgaaggcgtc acgctcatcc tggaaaaaga agtcgccctg gcccatcaaa
      901 tccaatttca tggcgaatgg gccaaaatta ccctcagggt tcattcttca ctcgaagccg
      961 tgggcttttt agccacaatt tcgcaaccac tggccgtggc tggaatcagc accaacgtca
     1021 tttcagcttt ttatcacgat catctttttg ttcaatggga taggcgcgag aaggcgattg
     1081 aaatcctcaa ccatctggct caaacggcag ctcacttgtg acgttggatt gcctgaaaat
     1141 catcgggtgt gttgacgttt atcaacaacc gatttgagtg cggaaaatcc gcatattcag
     1201 acgcccgaat acgccggact gtggtgtgtt caagcaaggt ttccatccgg cgttcaccct
     1261 gatcaagtaa tctggtcaca actggataaa ctgatgcctg atacacggca cacatgctgt
     1321 gaagccgtgg tttttgaact ccggtcgttt ctggaacgac aatctgggct tcctggcgat
     1381 agttaagcag cagagacaga aactcagccg tcaaaaaggg aagatcacac gccacgatca
     1441 gtgaaaatga acgagtggtg gcgttgagag ccgcttcaat tccagccagt gggcccgcac
     1501 cacgattggc atctcgacgt aagtcaccca gcagtggaac tccaagccta agataggttt
     1561 ctggagcatt ggtcacaatc gaaacatcat ctgtcacggc agaaagagtc cgcaacacgc
     1621 gctcaatcat cggtacgcct tcgaccagga ggcgggcttt gttgaccccc attcgccggc
     1681 tttccccacc agccagaata aatccactca catcaggaaa aagagttgaa ggcatcagta
     1741 gtcagtcgtc agtcgtcagt agttagtagt cgagtgttgg gaattgagac tttatgtcaa
     1801 gtgtagttgg ttctatttat gccatttatg caagtttctt tccaggaaat cattgagctg
     1861 aagtcccagg tttgatctac tgacgactga cgactgacga ctgacttgaa ttgctactt
//
LOCUS       DAOQDO010001346         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60639_length_1919_cov_2.128755, whole genome shotgun sequence.
ACCESSION   DAOQDO010001346 DAOQDO010000000
VERSION     DAOQDO010001346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60639_length_1919_cov_2.128755"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..166
                     /locus_tag="PKZ53_18695"
     CDS             8..166
                     /locus_tag="PKZ53_18695"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNJ42524.1"
                     /translation="MKTKKEILEKTRRRLKSDGVLFLGTAETTLNLDDSFVRSQFEQT
                     SYYQIKPR"
     gene            283..753
                     /locus_tag="PKZ53_18700"
     CDS             283..753
                     /locus_tag="PKZ53_18700"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNJ42525.1"
                     /translation="MKRYIPMNAEITTITESIWSAVLGFDLETKPVADNSVLSEPVLT
                     GVVHITGKWNGSICLHCPVTVARTFAARIFECSPESITDDELGDVVGELTNMISGNLK
                     PILPGPSLLSLPMVQTNVTISDKNGFASRDQLICLASFACAGHPFQVSVVEMQS"
     gene            complement(913..>1919)
                     /locus_tag="PKZ53_18705"
     CDS             complement(913..>1919)
                     /locus_tag="PKZ53_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ42526.1"
                     /translation="IEPARELRDGSAHPLGRREPPALVPSPKPDALTPNVITRPRRVT
                     GLNPKPLESTFSQSASASSSQECGMSPLSTTGLTPQTALRSTATRSQELQSGQPRHLD
                     HSVSPQRQLRPENSRRVGRQVVIQVPPASHPARLQTVSVTYRTTASAAPIRSEVEFSS
                     GKKAFPATIDTLSRESVPLQKSLPPNWVTGKKNIDPIIHQISQEHRLDPFLVAAVIRQ
                     ESGFKQYALSNKGASGYMQLMPGTFHRFGGRGKNIFDPVANIRAGVAYLRFLSDRYNG
                     NVDKVLAGYNSGEGNVDKYGGIPPFKETRNFVASVKQHYRQFVALAQSAATQSEVAMR
                     "
BASE COUNT          449 a          503 c          502 g          465 t
ORIGIN      
        1 ttttgatatt aaaaccaaaa aagaaattct tgaaaaaacc cgaaggcggt taaaatcgga
       61 cggcgttttg tttttgggaa ctgccgaaac cactttaaac ctcgatgatt cgtttgttcg
      121 gagccagttt gagcaaactt cctattacca aatcaaaccc cggtaaggaa aaagtcgcgc
      181 tatcgccttt gtaaccgtga tggttggggc atcgcgcacc agaaaaaacc ggcctgttcc
      241 aggccaatag acctgaacct tgcaatcgtt gccccaaacc ttatgaaacg gtacatccca
      301 atgaacgccg aaattaccac catcaccgaa agtatctggt cagcagtttt aggatttgat
      361 ctcgaaacca aacccgtcgc cgacaattct gtcttgagcg aacccgtcct caccggtgtg
      421 gttcacatta ccggcaaatg gaatggcagc atctgtttgc attgcccggt cacggtggca
      481 cgaacctttg cagccagaat ttttgagtgc tcacccgagt ctataactga cgacgaactc
      541 ggggatgtgg ttggtgaact caccaatatg atcagtggca atctcaaacc gatcctgccc
      601 ggccccagtt tgctttcgct gccgatggtc caaaccaacg tcacgatcag tgacaagaat
      661 ggctttgcat ccagggacca gctcatctgt ctggcttcat ttgcctgtgc tggtcatcca
      721 tttcaagtat cagtggtcga aatgcagtcc tgatcgggca ggccaggttt cttcccctgg
      781 cgcatcggcc ccgatacccg gaccccaaca atgatctgag ttcatctgcc caaaaaaaaa
      841 cggccacggg tttttcccgt ggccgttctt gtgttacgtg tcggcatcaa gatcataacc
      901 gccgcctggc gattaccgca ttgccacttc tgactgcgtg gctgccgatt gcgccagcgc
      961 tacaaattga cgatagtgct gtttgacgga ggcgacaaaa ttccttgttt ctttaaacgg
     1021 agggatgccg ccgtatttat caacattccc ttcgccagag ttatatccag ccaggacttt
     1081 atccacgttc ccgttgtatc ggtcacttaa aaatcgcagg taggccacac cggcgcgaat
     1141 attggcaacc gggtcaaaaa tattttttcc acgccctcca aagcgatgaa acgtgccggg
     1201 catcaattgc atataccctg aggcaccttt gtttgagagg gcatattgtt taaaaccaga
     1261 ttcctgacga ataacagccg ccaccagaaa tggatccagc cggtgttcct gactgatttg
     1321 gtgaataatc gggtcaatat ttttcttgcc cgtgacccag tttggcggca gggacttttg
     1381 taatggaacg gattcccgac tcagtgtatc aatcgtagcc gggaacgctt ttttgcccga
     1441 agaaaattcg acctctgacc ggattggtgc cgctgaagcc gtggttcggt acgtgactga
     1501 aacggtttga agtcgtgccg ggtgcgacgc aggcggcacc tggatgacca cctggcgacc
     1561 aacccgccgg ctgttttcag ggcgaagttg acgctgaggc gagactgagt ggtcaaggtg
     1621 ccggggctgg ccggattgaa gctcctggga tcgggtcgcc gttgaacgta gagccgtctg
     1681 tggcgtgagt ccagttgtgg ataacggaga catcccacac tcttgcgaac tggacgcgct
     1741 ggccgactgg ctgaaggttg attccagtgg tttgggattg agcccggtca cccggcgcgg
     1801 tcgggtaatt acatttgggg tcaaggcatc aggttttggc gaagggacaa gcgccggagg
     1861 ttctctacgt ccgagaggat gagccgatcc atcacgcagt tctcgcgccg gttcaattg
//
LOCUS       DAOQDO010001347         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60659_length_1918_cov_3.662909, whole genome shotgun sequence.
ACCESSION   DAOQDO010001347 DAOQDO010000000
VERSION     DAOQDO010001347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60659_length_1918_cov_3.662909"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1918
                     /locus_tag="PKZ53_18710"
     CDS             <1..>1918
                     /locus_tag="PKZ53_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNJ42527.1"
                     /translation="GIPPDTHGAVGPNHLVVATNQQVRIQSRTGSNISTVSLDAFWRS
                     LEPFSFTFDPKVLYDPYANRFMMTVMIDFGSPNSSVLLAVSKTSDPTGQWNFYKVDTD
                     STDALSADYPSIGFNKNWIAIQVNMFSPSSGRFTEAKLFVFDKADAYAGGAGRVTTIR
                     SPEFVLVPGITYSDTQDTLFCVGGSFNSSIRISTITGAVGSETLTVGATVSGGSDGWS
                     FNQPGFQDSAPQNGSAVRIDSGDGRMQNVVFRNGSIWCAHTIFRPGSSASRAAVQVWQ
                     LSTSGNVLQRIILEDPNSTIFYAYPSVAVNKNNDALIGYSRFSPTQFASANYAFRAGS
                     DAPNTLRDDTVLKAGESAYDKNPTFGANRWGDYSNTVVDPVNDTDMWTIQEFAAPGNR
                     FGTWWGRVALDPGAGDTTPPTVQLQSPNGGESVKGSERTTIKWTSNDNNGVIQHDLQL
                     STDGGTTFGIRIAAGISGSVQEFSWAVPLVETTRARVQITALDSFGNDATDTSDADFS
                     IARPPLSPAQNVTATVNGQTVQLAWEAARPTPGANITGYSIYRSRTSPVTVAASNRLG
                     TFSIQTRTFSDRPSPADGKTQYFYVVTGVYNTGESGPSNEVSATPESNNDTKPPVVQV
                     LAPNGGETVNNGEPL"
BASE COUNT          448 a          519 c          492 g          459 t
ORIGIN      
        1 gggattcccc ctgataccca tggtgccgtt ggccccaacc atctggtcgt ggcgaccaat
       61 cagcaggttc gcatccaaag ccgcactggc agcaatatca gcaccgtgtc gctggatgca
      121 ttctggcgtt cgctggaacc cttcagtttt acctttgatc cgaaagtttt gtatgacccc
      181 tatgcgaacc ggtttatgat gacggttatg attgactttg gctcaccgaa ctcttcggtg
      241 ctgctggccg tctcaaaaac cagtgacccc accggacaat ggaactttta taaagttgac
      301 acagattcaa ccgatgcgct ttcggctgac tatccgagca ttggatttaa caaaaactgg
      361 attgccattc aggtcaatat gtttagcccg tcctcgggtc ggtttaccga agcaaaactc
      421 tttgtttttg acaaagctga tgcctatgcc ggaggtgctg gccgggtgac gacgattcgc
      481 tcaccagagt ttgtgcttgt tccggggatt acctatagcg acacccagga caccttgttt
      541 tgcgtgggag gtagttttaa ttcatctatc cgcatttcaa ccatcacggg tgccgttgga
      601 tctgaaaccc tgacggttgg ggcaactgtc agcggcgggt cagatgggtg gtcctttaac
      661 cagcctggat tccaggattc agccccgcaa aatgggtcag cagtcagaat tgactccggt
      721 gatggtcgga tgcaaaacgt tgtgttccgg aatggttcaa tctggtgcgc ccatacgatt
      781 ttccgacccg gaagctcggc atcacgagcc gccgtccagg tctggcaatt gtcaaccagc
      841 ggcaatgtct tgcagcgcat tatcttagaa gatccaaaca gcaccatttt ctatgcttat
      901 ccaagtgtag ccgtcaataa aaacaatgat gcgctgattg gctattccag gttttcaccc
      961 acccagtttg ccagcgccaa ctatgctttt cgggctggtt cggatgcccc aaacacgctt
     1021 cgggacgata cggtattaaa agctggcgaa agtgcctatg acaaaaaccc aacgtttggt
     1081 gccaaccgct ggggagatta cagcaacacg gtggttgatc cggtcaacga cactgacatg
     1141 tggacgattc aggagtttgc cgcccctgga aaccggtttg gtacctggtg gggacgggtg
     1201 gcacttgatc caggtgctgg tgacaccacc ccgccgacag ttcaactgca atctccaaat
     1261 ggcggcgaat cggtcaaagg gagcgagcgt accaccatca agtggacttc aaatgacaac
     1321 aatggcgtga ttcaacacga tttacagctt tcaaccgatg gcggcacgac ttttgggatt
     1381 cggattgccg ctggcatcag tggctcggtt caagaatttt cgtgggcggt accgctggtt
     1441 gaaaccactc gcgccagggt tcaaattaca gcactcgata gttttggcaa tgatgcaacc
     1501 gataccagcg atgctgactt ttcaattgcc cgtccaccgc tctcgccagc ccagaatgta
     1561 acagccaccg tgaatggtca aactgttcaa ctggcctggg aagctgcgcg cccaactccg
     1621 ggtgccaata tcaccgggta tagcatctat cggtcacgaa cctctccggt aactgttgct
     1681 gcctccaatc ggctgggcac gtttagcatt cagacccgga ctttttctga tcgcccatct
     1741 ccggcagatg gcaagaccca atatttctat gttgtgacgg gggtgtacaa tacgggtgaa
     1801 agcggcccat ccaatgaagt ttcggccaca ccagagtcaa acaacgacac caaaccgccg
     1861 gtcgtccagg ttcttgctcc aaacggtggc gaaacagtca ataatggcga accactcc
//
LOCUS       DAOQDO010001348         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60692_length_1918_cov_2.434783, whole genome shotgun sequence.
ACCESSION   DAOQDO010001348 DAOQDO010000000
VERSION     DAOQDO010001348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60692_length_1918_cov_2.434783"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PKZ53_18715"
     CDS             complement(<1..772)
                     /locus_tag="PKZ53_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42528.1"
                     /translation="MWGQLGIAALQILITLSTILVFHYWRKRFGLGPLYIALGGLELF
                     VTFVTALWIKIDLPYGLAFVTGSTSFFVVGFTALLLVYLCEGTLQARNLIFSLIGLQA
                     CHIIWRLLVLAQLLVSGGGGLGGAIPTALFSPDFRLNMASAVARIVTLFAFIIFYQWL
                     SNRFSGWPILLKIFTSLLIALILDDLIFFTLGMFESPQFLSNLLGSILSRPLSVMLIS
                     PVLAMYLQLPQVSYRTTEDIRDPMDILQSNALLQASLAE"
     gene            complement(974..1549)
                     /locus_tag="PKZ53_18720"
     CDS             complement(974..1549)
                     /locus_tag="PKZ53_18720"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNJ42529.1"
                     /translation="MTFFDVLVLISLAYSTASGAMKGLVRSLSTTAGVVVGLMFALVF
                     YREAAQFFRMLGFSEVTALCLGMISPMVLALAASAVLSYSLREVLKKLGLTVLDRVGG
                     GALGLARAVLFWSLIYLAFTAFPIRLQFIEGAQTGPILKAGAQVLAFISSQDVEKRFD
                     QGVTELRKLKAGLSSPSPKEKAPQPDPKERR"
     gene            complement(1764..>1918)
                     /locus_tag="PKZ53_18725"
     CDS             complement(1764..>1918)
                     /locus_tag="PKZ53_18725"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813207.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNJ42530.1"
                     /translation="DKQFVRDYLEALDWNKQPPAPALPAQIVDATQARYLEAYERLTG
                     KQLFLS"
BASE COUNT          559 a          465 c          467 g          427 t
ORIGIN      
        1 actcggccag tgatgcctgc agcagcgcat tgctctgcag aatgtccatt gggtctcgaa
       61 tatcttcagt tgttcgataa gagacctgcg gaagctgtaa atacatggcc agcacggggc
      121 tgatcaacat cacactcagt ggacggctca aaatacttcc gagcagattt gacaggaact
      181 gtggtgattc aaacatcccc agcgtaaaaa aaatcagatc gtccagtatc agcgcgatga
      241 gcaggctggt gaaaattttc aagaggatgg gccagcctga aaatcggttt gaaagccatt
      301 gataaaaaat gataaaggca aacagggtca caatccgagc cactgccgag gccatattaa
      361 gccggaaatc cgggctgaac agggcggttg gaattgcccc tccaagccca ccaccaccac
      421 tgacaagaag ttgggccagc accagtaacc gccatataat atggcacgcc tgtaatccaa
      481 tcagtgaaaa aatcaagttg cgtgcctgca atgtgccttc acagagatac accagcagca
      541 gtgccgtaaa cccaactaca aaaaaactgg ttgacccggt tacaaaggcc agcccatatg
      601 gtaaatcaat tttgatccac agggcggtaa caaaggtcac aaacaattcc aacccaccca
      661 atgcaatata caacggacca agcccaaagc gcttcctcca gtaatggaaa accagaatcg
      721 tgctcaaagt gatgagtatc tgtaaagcag caatgccaag ctgaccccac ataagaaaaa
      781 cctctgggct gaagaaatta ggcttggggc tgagaaatcc agggctgaga gcctggggct
      841 gaagacatcg ggcttggggc tgagggctga agaaggcggg ctgaagagag cgggttgaag
      901 aactggtttt atgtcatccc ttcgattgcc caagcccgat attcttcagc tccaggttct
      961 tcagctcgct cagttatcgt cgctctttgg gatcaggttg aggggctttt tctttggggg
     1021 aaggtgaaga aagcccggct ttgagtttgc ggagttcggt cactccttga tcaaaacgtt
     1081 tttccacatc ctgtgacgag ataaaagcca ggacctgagc gccagctttt aaaattggtc
     1141 cagtttgggc gccttcgata aattgtaacc gaattggaaa cgcggtaaat gccagataga
     1201 tcaaagacca gaaaagcacg gcccgtgcca aaccaagcgc accaccacca actcgatcaa
     1261 gtacggttaa acccaatttt ttcaatactt cccgtaatga ataagaaagc accgcgctcg
     1321 cagccagggc aagcaccatc ggagaaatca tccccaaaca gagtgctgta acttcagaaa
     1381 agccaagcat ccgaaagaac tgtgccgctt cgcggtaaaa aaccagtgcg aacatcagcc
     1441 caacaaccac acctgccgtt gttgacaggc tgcgcacaag ccctttcatg gcgccgcttg
     1501 cggttgaata agccagtgaa atcaaaacca gtacatcaaa aaaagtcatg tgaagaagtt
     1561 gtcagttatc aattgtcagt cgttagtcgt cagtcatcag tcgtcagtca tcagtcgtca
     1621 gtcgtcagtc gtcaagtgtt gggatttggg aatttgtgtc aagtggattt ggttcttttt
     1681 gagtcattta cgaagagttc tttcaagatc tttgcagact gaaatacctg ggtttggtct
     1741 actgacgact gacgactgac gtatcaactc agaaacaact gtttcccagt taaccgttca
     1801 taggcttcaa gatagcgtgc ctgtgtggca tcaacaatct gggctggcaa agccggggct
     1861 ggtggctgtt tgttccagtc aagggcttcc aggtaatcac gcacaaattg tttatcaa
//
LOCUS       DAOQDO010001349         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60802_length_1916_cov_2.960774, whole genome shotgun sequence.
ACCESSION   DAOQDO010001349 DAOQDO010000000
VERSION     DAOQDO010001349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60802_length_1916_cov_2.960774"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..460)
                     /locus_tag="PKZ53_18730"
     CDS             complement(191..460)
                     /locus_tag="PKZ53_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42531.1"
                     /translation="MSKDDSFDNSTVDPFGSAAAPVFNSPFCGELRSKNYFLLDVLPT
                     SAEQYLDPSGHCWCFMTHQVVGPDGGKVHPTRCTSDRRCYKSALA"
     gene            complement(470..1480)
                     /locus_tag="PKZ53_18735"
     CDS             complement(470..1480)
                     /locus_tag="PKZ53_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNJ42532.1"
                     /translation="MKAFEWVNPTTVAEAVKQLSTQVSDPKEAPRPLAGGQDLLTTMK
                     DYITQPVRVVNLKSIPGLNKIEGDAKKGLKLGALVTLSQLEEHPEIRKSFPAIAEAAH
                     SVASTQIRNLGTLAGNLCQRPRCWYFRLEHVVCLKKGGDQCYAEAGENKYNAILGGGP
                     SYIVHPSDLAPALVALGASVVIVGPNGNRTVELEKFFKLPSQGDLLRENVLADNEIIT
                     EIQVPASPVAARSTYLKFKERDSLDFALSAVAAAVELDAGGKVKQARLVLGGVAPTPW
                     RVPKAESYLVGKKLDEATLQEAAKLALEGAQPLLHNQYKVPLTQTLVRRALNQLGSGK
                     SA"
     gene            complement(1493..>1916)
                     /locus_tag="PKZ53_18740"
     CDS             complement(1493..>1916)
                     /locus_tag="PKZ53_18740"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ42533.1"
                     /translation="GVCFAEVEVDVETGHVKPIKMVHVQDIGLPLNRLAVESQINGGM
                     IQSLGMALYEGRVVDSQLGITLNPGFGDYKLPGSLEMPELVPIIDDEDTREAVIGVGE
                     PALIPAVGALANAVFHACGVRVRELPITPDKILMGLFK"
BASE COUNT          427 a          560 c          477 g          452 t
ORIGIN      
        1 ggtgaccact tccccgttgt aatggcaggc accggcaaag acactcgccg tccgggcctc
       61 cacgtaatca cctgaaattg ctcccgcctt tggctcgctt ccaaaacctg gaaggaccat
      121 ggccagaagc atcgcactcg tagctaacca tttcttttgc ataccacaaa ctccattgat
      181 gcgcagtccc ttacgccagc gccgatttgt aacaccttcg gtctgaagta catcgggttg
      241 gatggacttt ccctccgtcg gggccgacca cttgatgcgt cataaaacac cagcaatgac
      301 cgcttggatc caaatattgc tcggcactgg ttggcaggac atcgagcaga aaatagtttt
      361 ttgaccggag ttcaccacaa aacgggctat taaacaccgg ggccgccgca ctcccaaacg
      421 ggtcaacggt tgaattgtca aatgaatcgt ctttgctcat accccctcct tatgctgatt
      481 tgccgctgcc aagctggttg agggctcgtc gaaccagcgt ctgggtcaac ggcaccttgt
      541 actggttgtg caagagtggc tgggcaccct ccagggccag ttttgcggct tcctggagcg
      601 ttgcctcatc cagttttttc ccaaccagat agctttcggc ttttggaacc cgccacggcg
      661 ttggggccac gccacccagg accagccggg cctgcttgac cttaccaccg gcatccagct
      721 caaccgcggc agccacggct gagagtgcaa aatcgagcga atctcgttcc ttgaatttta
      781 aataggttga tcgagccgca actggtgaag ccggcacctg aatttcggtg atgatttcat
      841 tatcggccag aacgttttcc cgcagcaggt caccctggct gggcagtttg aagaatttct
      901 caagttcaac cgttcgattc ccatttggcc caacgatcac cacactggcc ccaagtgcca
      961 ccagtgccgg cgccaaatca gatggatgga cgatatagga cggcccacca cctaaaatgg
     1021 cgttgtattt gttttcaccc gcctcggcat aacactgatc accacctttt ttcaggcaga
     1081 ccacatgttc cagccgaaaa taccagcacc ggggacgctg acacaggtta ccggccaggg
     1141 tcccgaggtt tcgaatttga gttgacgcca ccgaatgcgc ggcttcggca atggcgggaa
     1201 agcttttacg aatctccgga tgttcttcca gttgtgagag cgtaaccagg gcacccagtt
     1261 tgagtccctt tttggcatcg ccctcaattt tattgagtcc gggaatggat ttcaggttca
     1321 ccacgcgcac gggctgcgtg atgtaatctt tcatcgtcgt cagcagatcc tgaccaccag
     1381 ccagaggccg gggcgcttct ttcgggtcgc tcacctgggt cgaaagctgt ttgacggctt
     1441 cagcgaccgt cgttggattt acccattcaa atgccttcat gtcggtttcc ccctatttga
     1501 acaaacccat taaaatttta tcgggcgtga ttggcaattc ccggactcga accccacagg
     1561 catgaaatac ggcattggcc agcgccccga cagccggaat caaagcgggt tcaccaacac
     1621 cgatgacggc ttcacgcgtg tcttcatcat caatgattgg aacgagttcg ggcatttcga
     1681 gtgatccagg cagtttatag tcgccaaacc caggatttaa ggtaattccc agttgcgagt
     1741 ccaccacccg gccttcgtag agtgccattc cgagggattg gatcatcccg ccgttgatct
     1801 ggctttcaac tgccagccgg ttgagcggga ggccaatatc ctggacatgc accattttaa
     1861 tgggtttaac atggccggtt tcgacatcaa cttcgacttc ggcaaaacaa acaccg
//
LOCUS       DAOQDO010001350         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60868_length_1915_cov_2.644086, whole genome shotgun sequence.
ACCESSION   DAOQDO010001350 DAOQDO010000000
VERSION     DAOQDO010001350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60868_length_1915_cov_2.644086"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKZ53_18745"
     CDS             <1..817
                     /locus_tag="PKZ53_18745"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42534.1"
                     /translation="VIVGAGYLGLLVLLYLGQEWLMFPGKTNQGTKYAHVKPGEGEEL
                     LTLPTPAGDTVAALFGKALTPDGKIRLDARQCPTLIYFYGNGMCLPDALYEFGEYRRL
                     GVNMIVPDYLGYGLSSGTPSEQGCYAAADAVYEHLHLRTDIDQKKIVALGRSLGSGVA
                     VDLAARKPLAGLITFSAYTSMVEMGKTSYPVVPVSLLLKHRFESIGKIQQVTCPILFG
                     HGKRDDVIPYTMTHELAQKAKAPVTEFYVDHLGHNDFWGETTIHPLQKFLSQF"
     gene            1084..>1915
                     /locus_tag="PKZ53_18750"
     CDS             1084..>1915
                     /locus_tag="PKZ53_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015139311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNJ42535.1"
                     /translation="MSFAKTAFAVETSALAKAIEKAQQYMLSQQYPEGYWWAELEANV
                     TLTAEYVMLHTILGTGAHRPLRKVIPYLFNRQCDHGGWELFYGDGGELSTTVEAYFAL
                     KLLGIPTDDPRMHRARTFILDRGGIPKTRIFTKLHLALFGAFDWNGIPTLPAWIMEFP
                     EWFPFTIYEMASWARSSTVPLLIVCDKKPVWRTANATVDELYVGGRDAADLSLPCEDG
                     FFSIGNTFVQLDKLFKFAEKIGFVPLREKALKHAEQWVLEHQDEPGDWGGIIPAMLNS
                     L"
BASE COUNT          451 a          462 c          498 g          504 t
ORIGIN      
        1 ggtcatcgtc ggagcagggt atctcggatt gttagtcctg ttgtaccttg ggcaagagtg
       61 gttgatgttt cctggaaaaa ccaaccaggg aacgaaatat gctcacgtga agcctgggga
      121 aggtgaggaa ctccttacac ttccaacgcc tgctggcgat acagtggctg cgttgtttgg
      181 gaaagcgctc actccggacg gcaagatccg ccttgatgcg aggcaatgcc caaccctgat
      241 ctatttttat ggcaatggaa tgtgcctgcc agacgccctg tatgaatttg gtgagtaccg
      301 ccggctgggc gtcaacatga tcgtccctga ctatctcggg tatggcctga gttctggaac
      361 tccttcggaa caggggtgtt atgcggcggc agatgcggtc tatgaacatt tgcatttgcg
      421 aactgatatt gatcaaaaga aaattgtagc tctgggacgg tcacttggtt ccggagtcgc
      481 agttgacctg gctgcccgca aaccacttgc cgggctcata accttcagtg cctacaccag
      541 catggtcgaa atgggaaaga cgagctatcc agttgttccg gtttcattgc tgctcaagca
      601 ccggtttgaa agcatcggaa aaattcagca ggtgacgtgt ccaattttgt ttggtcatgg
      661 gaagcgggat gatgtgattc cctatacgat gacccacgag ctagcccaaa aagcgaaggc
      721 gccagtgacc gagttttatg tggatcacct ggggcacaat gatttctggg gtgaaaccac
      781 gatacacccg cttcagaaat tcctgagtca gttttgattg agtcatcagt ttgctgcgtc
      841 cggtcttcaa tctccgaacc ccaattgcca cgagaccttt gaataacctc cctgttcgcc
      901 tattatccag aatcttttgt ctgcttcaga cgccccatcc caatagattg aagaaagtgt
      961 tatgccagcc tcaactcatt ctctggtatt cgtcgctggc taagtgcgtt taataaaccc
     1021 acttagcaaa ttactgatta ccggtttatt gacgactggc tggtattgag aggtcgaata
     1081 tccatgagtt ttgcgaaaac tgcctttgcc gtagagacat ctgccctggc aaaagccatc
     1141 gagaaagcac aacagtacat gctcagtcag caatatccag aagggtactg gtgggcagaa
     1201 cttgaagcca acgtcacctt gactgccgaa tacgtcatgt tgcacaccat tctgggcacc
     1261 ggtgctcatc gtccgttgcg aaaggtcatt ccgtacttat tcaatcggca atgcgaccac
     1321 ggtggatggg agttgtttta tggtgacgga ggggaactca gcacaacggt tgaagcctac
     1381 tttgctttga aattactcgg aatcccaacc gatgatcccc gcatgcaccg tgcccgcacc
     1441 tttatcctgg accggggtgg gattcccaaa acccggattt tcaccaaact tcatctggca
     1501 ctgtttggag cttttgattg gaatgggatt cctactcttc cagcctggat catggaattt
     1561 cccgagtggt tcccctttac gatttatgaa atggcaagct gggcgcgcag cagtaccgtt
     1621 ccgttattga ttgtgtgtga caaaaaacca gtgtggcgta ccgccaatgc caccgttgat
     1681 gaattgtatg ttggtggccg tgacgctgcc gacctgtcac ttccgtgtga ggatgggttc
     1741 ttttcgattg gaaatacctt tgttcaactc gacaagttgt tcaagtttgc cgaaaaaatt
     1801 gggtttgttc cactccgcga gaaagcgctc aagcacgccg aacagtgggt gttagagcat
     1861 caggatgaac ctggtgattg gggaggcatt attccggcga tgttaaattc attgc
//
LOCUS       DAOQDO010001351         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60903_length_1914_cov_3.522324, whole genome shotgun sequence.
ACCESSION   DAOQDO010001351 DAOQDO010000000
VERSION     DAOQDO010001351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60903_length_1914_cov_3.522324"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKZ53_18755"
     CDS             <1..275
                     /locus_tag="PKZ53_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ42536.1"
                     /translation="QQRVAIARAIAKRPEVLLCDEPTGALDYQTGKLVLEVIEQVNRD
                     LGTTTAVITHNAAIAEMADRVVRMRSGEIVEITVNQIKKHPADLIW"
     gene            431..>1914
                     /locus_tag="PKZ53_18760"
     CDS             431..>1914
                     /locus_tag="PKZ53_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ42537.1"
                     /translation="MATLDRKLIRDLAHIRGQLIAIALVVACGVSAFVSMGSTYFSLL
                     HTQQAYYDQFRFADVFAQLKRAPDTLSTQIAAIPGVASVRTRIVMEVTLDLPGLAEPA
                     TGRLVSLPERQTMMLNDLFLRQGRYIEPDHPDEIIASEAFANANQLQVGDGFKAIING
                     RLRPLRIVGIALSPEYIYEVRGGGSIFPDNKRFGILWMSHKVLATAFNLDGAFNDVAI
                     RLSPNASEPEVIAQLDRLLTGYGGLGAYGRDEQTSNRFISDEIAQNKFSSTIIPLIFL
                     SVASFLLHIILSRLVNIERNQIAILKAFGYRNFTIGVHYLKLALVPVWGGVIFGTLSG
                     TYLGQKLTELYTLYYHFPFLRYEASLELFAFAILITLSTAGLSVVSALRSAVNIPPAE
                     AMRPEPPAAFNQGWLDKLGIHRLFPPAIRMILRNIERRPIKAFLSVLGVAVSVAILVI
                     GMYFFDAFDHLMKVQYEFVQRQNVTVAFNEVRPPRVIHELERLPG"
BASE COUNT          444 a          507 c          486 g          477 t
ORIGIN      
        1 agcaacaacg cgtcgcaatt gcccgggcga ttgccaagcg ccccgaagtc ctgctctgtg
       61 acgaacccac cggcgcgctc gactaccaga ccggaaaact ggttctcgaa gtcatcgaac
      121 aggtcaaccg tgacctgggc accaccacgg cggtcatcac ccacaatgct gccattgctg
      181 aaatggctga ccgggtggtg cggatgcgca gtggcgaaat tgttgaaatc actgtcaatc
      241 agatcaagaa gcatccggca gacttgattt ggtaagtacc ttgccgaaaa tttccagaca
      301 ttttaaccac ggaaaacacg gaaaacacgg aaaaaaatca agcacttacc aaacccaatg
      361 tctcaggaag cttatgacaa ggtacttaag gcaatacagc ggcgtcaagt gagccgcact
      421 tcgaaaatat atggcaacgc tcgaccgcaa actcattcgg gatttggcgc atattcgagg
      481 gcaattgatt gctattgcgc tggtggtggc ctgcggggtg tcggcgtttg tttcgatggg
      541 aagcacctat ttttccctgc tccataccca gcaagcctat tatgaccagt ttcggtttgc
      601 agatgtgttt gcccaactca agcgagcccc cgacacgctg agcactcaaa ttgcggcaat
      661 tccaggtgtg gcctcggttc gaacgcggat tgtgatggag gtcacgctcg atctgcctgg
      721 tctggccgaa ccggcaaccg gacggctggt gtcgcttccc gaacgccaga caatgatgct
      781 caatgacctc tttttgcggc aggggcgcta tattgaacct gatcacccag acgaaatcat
      841 tgccagtgaa gcgtttgcca atgccaatca attgcaggtg ggcgatggat ttaaagccat
      901 catcaatggc cggttgcgtc cgctccggat tgtcggaatt gcgctgtcac cagaatacat
      961 ttatgaagtc cggggtggag gttcgatttt ccccgataac aagcggtttg gcatcttgtg
     1021 gatgagtcac aaggttttgg caacggcatt taatctggac ggcgccttta atgatgtcgc
     1081 gatcagattg tcgcccaatg cttcggaacc agaggtcatc gcccaactgg atcgcctgtt
     1141 gaccggttat ggggggcttg gggcgtatgg ccgtgacgaa caaacctcca accggtttat
     1201 ttcagatgaa attgcccaga acaaatttag cagcacgatc attcccttga tttttttgag
     1261 cgtcgcatcc tttctgctgc acattatttt gtcacgcctg gttaacatcg aacgcaacca
     1321 gattgccatt ttaaaagcct ttgggtatcg caacttcacc attggcgtgc attacctcaa
     1381 gctggcgctg gtgccggtgt ggggtggagt gatctttgga acactcagtg gcacctacct
     1441 tggccaaaaa ctgaccgaac tgtacacgct ctattaccat ttcccatttt tgcgctacga
     1501 agccagtctg gaactttttg cttttgccat tttgatcacc ctttcaaccg cagggttgag
     1561 cgtggtgagc gccttgcgca gtgccgtcaa tattcctcct gccgaagcca tgcgccccga
     1621 accacctgcc gcattcaacc agggatggct cgacaaactg gggattcatc gcctgtttcc
     1681 accagccatc cggatgattc ttcgaaatat cgaacgccgc ccgatcaaag cctttctctc
     1741 agtacttggc gttgccgttt cagttgcgat tctggtcatt ggaatgtact tctttgacgc
     1801 ctttgatcac ttgatgaagg tgcagtatga atttgttcag cgtcagaatg taaccgttgc
     1861 tttcaatgaa gttcgcccgc cacgagtaat tcacgaactg gagcgattgc ccgg
//
LOCUS       DAOQDO010001352         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60954_length_1913_cov_5.187836, whole genome shotgun sequence.
ACCESSION   DAOQDO010001352 DAOQDO010000000
VERSION     DAOQDO010001352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60954_length_1913_cov_5.187836"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1611
                     /locus_tag="PKZ53_18765"
     CDS             58..1611
                     /locus_tag="PKZ53_18765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ42538.1"
                     /translation="MHKLACLGFLALLPFLLASGCRRNQQLTPGLLVMATEKAPKSID
                     PRVGNDSVSARLHQLLFNTLVTKNDKLEIVPELATFEVSPDAKTFTFHLKPGVMFHNG
                     KPLTAEDVKYTFDTLRDPNFNSAKKGDFIKVEVIEAPDPQTVIFRCKEPNSPMLVSLV
                     AIGIIPKDSGATAETKPIGTGPFKVGDYQVDREITMEAFPQYFEGAPKLEKVRVRFIP
                     DSAVRELELRQGGVQLAVNADLASDTVEKLAQTPNLTVWQSTGTNISHLGLNCDDPIL
                     KNVKVRQALVHAINRESFIKNVLRNMARPAYGPLPPEQWAYPADLPKLDYDPALAKKL
                     LDEAGYRDPDGDGPQTRLKLEIKTSSLPLARQIATVLQEQFKQVGIQLEIRSFEFQTF
                     LSDVNAGNFQLYTLTSIGANQHPDYLSYAFDSSRIPTKEKNYSEGANRSHYRNAEVDG
                     WLRKAGATLNQEEQKQLYGLAQKKIVEEVPTIFLWYPNNVAIGNNKLTNVKPDLSGSY
                     TFLRNSTFQ"
     gene            complement(1650..1820)
                     /locus_tag="PKZ53_18770"
     CDS             complement(1650..1820)
                     /locus_tag="PKZ53_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42539.1"
                     /translation="MKPSYYSFSEKDSPLGTPASCRQLLEPQPIDAGRMPALPGNIYL
                     KCYSKPLSCSTG"
BASE COUNT          512 a          506 c          443 g          452 t
ORIGIN      
        1 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc ctaacctatg
       61 cacaaacttg cctgtcttgg attcctcgct ttgcttccgt ttttactcgc ctcgggttgc
      121 cgacgcaacc agcaactcac gcctggattg ctcgtgatgg caactgaaaa agcgcccaag
      181 agtattgacc ctcgggttgg caacgacagt gtttcggccc ggttgcatca gttgctcttt
      241 aacacgctgg ttaccaaaaa cgataagctc gaaattgtcc cagagctggc gaccttcgaa
      301 gtatcaccag atgcaaaaac cttcacgttt catttgaaac ctggggtgat gttccacaat
      361 ggcaagccac tcaccgccga agacgtcaaa tacacgtttg acacgctccg cgacccgaat
      421 ttcaactccg ccaaaaaggg tgattttatt aaggttgagg tgattgaagc ccctgaccca
      481 caaacagtca tctttcggtg caaagaaccc aattccccga tgctggtcag cctggtggca
      541 attgggatca ttccaaaaga cagtggtgcc acggctgaaa ccaaaccaat tgggactgga
      601 ccgtttaaag taggcgatta tcaggtagac cgtgaaatca ccatggaagc cttccctcag
      661 tattttgagg gggctccgaa gcttgaaaaa gttcgcgtcc gatttattcc agatagcgcc
      721 gtgcgtgaac tcgaactccg tcaaggcggc gtccagttgg cggtcaatgc cgatctggct
      781 tcagatacgg ttgaaaaact ggcgcaaact ccaaatctca cagtctggca aagcactggc
      841 accaacattt cacatctcgg actcaattgt gatgacccga ttttgaagaa tgtcaaagtc
      901 cgacaggcgc tagtgcatgc cataaaccgc gagagtttta tcaagaatgt cctgcgcaat
      961 atggcccgtc cggcctatgg accacttccg ccggaacaat gggcgtatcc ggctgacctg
     1021 ccaaaacttg actatgaccc ggccctggcc aaaaagctgc tcgacgaagc cgggtaccgc
     1081 gacccggatg gagacgggcc gcaaacacgg ctcaaactgg aaatcaaaac gtcttccctg
     1141 ccgttggctc gtcagattgc cacggtgttg caggaacagt ttaagcaagt cgggattcag
     1201 cttgaaatcc gatcttttga atttcagacg tttttgagcg atgtcaacgc cgggaatttc
     1261 cagctttaca cgttgacgag catcggcgcc aatcagcacc ctgattattt gagttatgcg
     1321 tttgattcgt cgcgcatccc caccaaagag aaaaactata gcgaaggcgc caaccgcagc
     1381 cactatcgta acgctgaagt tgacggctgg ttgcgcaaag ccggggcaac actcaaccag
     1441 gaagaacaaa agcaactcta cgggctggca caaaagaaaa tcgtcgaaga agtccccacc
     1501 attttcctgt ggtatccaaa caacgttgcc atcggcaaca acaagctgac aaacgtaaag
     1561 cctgatttgt caggaagtta cacatttttg agaaattcga cttttcaatg acaaaataat
     1621 accagtttgt agtcagtagt ccgctaagtt tatccggttg aacaacttaa cggcttgcta
     1681 tagcatttca gataaatatt tcctgggagc gccggcatcc tgccggcatc aatcggttgc
     1741 ggttcaagca gttgccggca ggatgccggc gttcccaggg gggaatcttt ttctgaaaaa
     1801 ctataatacg aaggtttcat tgcaaattgg tataaccatt tgaaatcaat gagtttcaat
     1861 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattcttc att
//
LOCUS       DAOQDO010001353         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60961_length_1913_cov_3.648009, whole genome shotgun sequence.
ACCESSION   DAOQDO010001353 DAOQDO010000000
VERSION     DAOQDO010001353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60961_length_1913_cov_3.648009"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="PKZ53_18775"
     CDS             complement(<1..103)
                     /locus_tag="PKZ53_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ42540.1"
                     /translation="MLQVGQKAPDFNMASTKDLNTLAENVKLSDYAGK"
     gene            651..1544
                     /locus_tag="PKZ53_18780"
     CDS             651..1544
                     /locus_tag="PKZ53_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42541.1"
                     /translation="MAKLTWEEFIDAVERLKTAQVPVTILNIRTEVGRGSYSTIQKFI
                     QKLASEEPEAGVGQTVRNREPLRSPLPETKKKEEWEGKVPDWFQQVTQTFAREVSFAA
                     YTWVQQEVDSVVMTQVLEANEAEDRVQELETSARELQLQLDRVAGECELLQGENQRLK
                     NHTFDLQQEKEELQRQLLLAQTRANEEERKSIEAQTRAAQSASLVLSLERALEAERSE
                     KDSLVLKFEKVQREYAEALARLDEVKGQNTGGRIEALEKRNQQLLEQLARERTAMATA
                     QAQALREKSRAEELQSRMRRG"
     gene            complement(1723..1804)
                     /locus_tag="PKZ53_18785"
     tRNA            complement(1723..1804)
                     /locus_tag="PKZ53_18785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1768..1770),aa:Leu,seq:tag)
BASE COUNT          550 a          397 c          530 g          436 t
ORIGIN      
        1 attttccggc gtaatccgag agttttacat tttcagccag agtattaagg tctttcgtgc
       61 tggccatatt gaaatcggga gctttctgac cgacttgcag catagtgtgg attctccttg
      121 tgagcttaaa agttggtata agaaagtaaa atcagtgcgg tctcgcgggg ttgaggttct
      181 gaagcaccgg gacactcttt cgggggaatc atttttccag ttccaaatga agtcgccaga
      241 gtggaaggtc gtgaccgcga accggttggt gtgatctccc gcccagtatc aggtcgggag
      301 gcgaaacaat atccattctg aagggttgca gcaatgattt ttcgccatcc gatacccacc
      361 ggctggtgga tcaacgtctc agacgccttg actggacgtt gtcagtgatt gccctccttc
      421 agaatttacg gttgcgtctg taattacatt tatggaatca tcggcaagtg ccaggcgaaa
      481 tggagatcgt atgaccgggt acgatactat catttggggc tgagggcatt gggctaagga
      541 ttgaggaaaa tacaagtatt ccaatgattt agccttcccg taatacgggg tatttattgc
      601 aaaatagtag aattaccttg aacagaaaag gaattgagtg caaatccgct atggccaaac
      661 taacctggga agaatttatt gatgcggtcg aacggttaaa aacagctcag gtgccagtca
      721 caatcctgaa cattcgaact gaggttggac gcggttcata ctccacaatc caaaaattta
      781 ttcaaaaact ggcttctgaa gaacctgagg ctggcgtggg acagactgtc cgaaatcgtg
      841 aaccactccg atctccactg cctgaaacca aaaagaagga ggagtgggag ggaaaagttc
      901 ctgattggtt tcagcaggta acacagactt ttgcccgtga ggtctctttt gcggcataca
      961 cgtgggtgca gcaggaagtt gattcggtgg tgatgaccca ggtgctcgaa gccaacgagg
     1021 ctgaggatcg ggttcaggaa ctggagacgt cggcgaggga actccaactt cagttggatc
     1081 gggtggctgg cgagtgcgag ctgttacagg gagaaaacca gcggttgaaa aatcatacgt
     1141 ttgatcttca acaagaaaaa gaagaacttc agcggcagtt attgctggct caaacccggg
     1201 ccaatgaaga agaacgtaaa tcaatcgagg cccagacccg ggccgcgcaa agtgcctcgc
     1261 tggtcctttc gctggaacga gccctggagg ctgagcggtc tgaaaaagac agcctggtcc
     1321 tcaagttcga aaaagtacag cgggaatatg ccgaagcact ggccaggttg gatgaagtca
     1381 aggggcaaaa tactggaggt cggattgaag cgctcgaaaa acgcaatcaa caactcttag
     1441 agcaactggc acgggagcga accgccatgg cgactgccca ggctcaggca ttacgcgaga
     1501 agtcacgggc tgaagaatta cagtccagaa tgcgcagggg ataacgcaat gatgacgcga
     1561 ggcgcttcag tcgtgcgaat taagggttga aattgagact tcaaaatcaa caacttacag
     1621 acggcagatt gaacgtctaa aacttgagat tttgataagt cttttgtttt caacgaacag
     1681 ttttttcaac cctgaactgg catcagtctg attgtggtgt gatgacccgg gtgggactcg
     1741 aacccacacc tcttgcgaga caggcttcta agacctgcgt gtctaccaat tccaccaccg
     1801 ggccggggaa tgaaatgaag aatgaagaat tgggaatgaa gaattgagaa tgaagaatga
     1861 agaattgaga atgaagaatt gagaatgaag aatgaagaat tgagaatgaa gaa
//
LOCUS       DAOQDO010001354         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_60998_length_1913_cov_2.193219, whole genome shotgun sequence.
ACCESSION   DAOQDO010001354 DAOQDO010000000
VERSION     DAOQDO010001354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60998_length_1913_cov_2.193219"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..>1913
                     /locus_tag="PKZ53_18790"
     CDS             557..>1913
                     /locus_tag="PKZ53_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ42542.1"
                     /translation="MKPTDSFAPMILIADDQAHVLEALRLMLKAEGYKIVSAASPSAI
                     LDAIGKQDFDVVLMDLNYARDTTSGQEGLDLLSRIKAVDSTLPVVVMTAWGSVELAVE
                     AIRRGARDFVQKPWDNERLLTILRNQIELGQALRRGQRLEAENRMLRNEGYPKLIADS
                     ASMRPVLQMIERVGPSDANVLITGENGTGKGLVARALHAVSLRTGKPLVTVNAGGLSE
                     GVFESELFGHVKGAYTDAKADRVGRFELADSGTLFLDEIANVPLNLQTKLLRVLETGE
                     FERVGSSRTRKVSVRILSATNADLSEEVKNGRFRQDLLFRLNTIEIRLPPLRERREDI
                     PLLALHFLTQHTQHYRKSLNGFDQHSMQALLNHGWPGNVRELDHVIERAVLMAQGKVV
                     QVGDLGLQAFRESSPRLEEMSLEEVECLLIKKALARHDGNVSRAAETLGLSRSALYRR
                     LQ"
BASE COUNT          488 a          457 c          534 g          434 t
ORIGIN      
        1 tttgcgaaaa aacagaatgt gggaacatag ccaccagaaa cggtacccag agcaaactgg
       61 ccaacagaag tttgggcaag cgcattggtt cacctcttat gaaaagtaag ggggggaagc
      121 cttagcttgc ctgttcctct atcaccaggt caatccaggt ctcggaaaat agagttccgg
      181 ctgaatcaac tgccccttct atctggagcg tgaacgccgg gcgtatttcc attggtggac
      241 aacaaaaatc ccaaaaccgg gcacctgcca gatatggtag tatgcaggcg gtgcaatcta
      301 acagagtcca attgcccagg ataaggccag cccaggtgat agtgtttgag agcgttcgat
      361 tgaggaaaga cttgtaatcc gagaggttac cagcttggaa taccagcatt ctgagtggtg
      421 atctctcgtt gctcaaaaac tctggcaacg aacacaaacg acctggatgt atctgatgtc
      481 atggcacatt ggttgcactt ctgaaccgtt tcatcttgag aaatacccag ggacccgaaa
      541 cctcactaaa aaaattatga aaccgaccga ttcatttgcc ccaatgattt taattgccga
      601 cgatcaagcc catgtgctgg aagccctccg attaatgtta aaagcggaag gctacaaaat
      661 tgtttcggct gcatctccat cggcaattct ggatgcgatt ggaaaacagg atttcgatgt
      721 cgtgctgatg gacctcaatt atgcgcgcga cacaacctct ggccaggaag gactggattt
      781 actgtcacga atcaaagctg ttgatagcac gctgccggtg gtggtgatga cggcctgggg
      841 gagtgttgag ctggccgtcg aagcgattcg ccgtggtgcg cgcgattttg tccaaaaacc
      901 ctgggataat gaacggctgt tgacgatttt gcgcaaccag attgaacttg gtcaggcgtt
      961 acgacgggga cagcgtctgg aagctgaaaa ccggatgttg cgcaatgaag gatatcctaa
     1021 actgattgct gattccgcca gtatgcgccc tgtgttgcaa atgattgaac gggttggacc
     1081 ctctgatgcc aacgtgttga ttactggcga aaacggtact gggaaaggtc tggtggcgcg
     1141 agcgctccac gcggtgtcac tgcggaccgg gaagccgctg gtgacggtca atgccggagg
     1201 gctctccgaa ggtgttttcg aaagtgaact gtttggccat gtcaaaggcg cctataccga
     1261 tgccaaagct gaccgggtag gccggtttga actggctgac agcggaacat tgtttctgga
     1321 tgaaattgcc aacgtaccgc ttaatttgca aaccaaactg ttgcgagtac tggaaactgg
     1381 cgaatttgaa cgggttggat catcgcggac acgaaaagtc agtgtgcgga tcttgtcagc
     1441 caccaacgcg gacctgagcg aagaggtcaa aaatggccgg tttcggcaag acctgttatt
     1501 tcggttgaat acgattgaaa tccgcctgcc cccgcttcgt gaacggcggg aagatatccc
     1561 gcttctggcg cttcatttcc tgacccagca cacccagcac tatcgaaagt cactcaacgg
     1621 gtttgaccag cactccatgc aggcattact aaaccacggc tggccaggga atgttcggga
     1681 acttgaccac gtcatcgaac gggccgtgtt aatggcacaa gggaaggtgg ttcaggttgg
     1741 tgatttgggg ttacaggcat ttcgcgagtc ttcaccacga ttggaagaga tgagtcttga
     1801 agaggttgaa tgcctgctga tcaaaaaagc actggctcgc cacgatggca atgtcagccg
     1861 ggctgccgag accttagggt tgagccgaag cgcactgtat cgccgcctcc agc
//
LOCUS       DAOQDO010001355         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61028_length_1912_cov_2.929456, whole genome shotgun sequence.
ACCESSION   DAOQDO010001355 DAOQDO010000000
VERSION     DAOQDO010001355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61028_length_1912_cov_2.929456"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKZ53_18795"
     CDS             complement(<1..560)
                     /locus_tag="PKZ53_18795"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNJ42543.1"
                     /translation="MTPRLVIYDAATRAWLRFQQPVQILTTSSVDDVLPLMQSIDQLV
                     NEQGYFAAGFLSYEAASGFDPALPTHPPGSFPVLWFGIFDRVERLTLPEPATVAGIDC
                     QWVPSISRVEYERAFHQVKQYLGRGETYQINFTLRLKSHFHGNPWDWFLTLAQAQQAD
                     YAAYVDLGPLVLCSASPELFFQLDGQ"
     gene            587..859
                     /locus_tag="PKZ53_18800"
     CDS             587..859
                     /locus_tag="PKZ53_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42544.1"
                     /translation="MKNDLNFAPKSTTRLKDQRRPVRDTTARRESARSVSEFEGHRNR
                     AFTVTKPTSTLFFLSFSSFSSFDFGLIRYQNQGTDQYFGRNLGNEA"
     gene            complement(892..>1912)
                     /locus_tag="PKZ53_18805"
     CDS             complement(892..>1912)
                     /locus_tag="PKZ53_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42545.1"
                     /translation="YHWRMHQDSASLNYQAKPYAYVAAQKAISQALTRRGEPGSVQIN
                     IRHPGYYTIRFDRPAAVSVGLIVFGTADIKQLVASVESWFTKTTISPVSVVSCLVEAQ
                     HQYLKKLIPTTVQSVHRVEPSSQTLNPELLNQAVRQAGTEIIVFLKAGLVVESDEWLE
                     LLVEQAYRPAIAAVGGILLQPGRLVRSAGLGLNRDTIAAELHQSLPGTAFGYHGSLIT
                     SHNVAALPLTGLACRREVFVASGGLNSQLKARFWDVDLCLRMFNEGYRHVCVPQVRML
                     DPEPVRSRQTRLNHDPDAEWMRRQWGAQLERDPYYNPNLSRQPMNFLIRSSFEAEQRP
                     RHKPR"
BASE COUNT          485 a          516 c          495 g          416 t
ORIGIN      
        1 tgttgcccat caagctggaa aaacaattcc ggtgaggctg aacacaaaac cagcggccca
       61 agatcaacat acgccgcata gtcagcctgt tgcgcctggg ccagcgtcaa aaaccagtcc
      121 cagggattgc cgtgaaagtg tgacttcaat cgcaaggtga aattgatctg atacgtctca
      181 ccccgaccaa gatactgttt gacctgatgg aatgcccgct catattcaac ccgactgatc
      241 gaaggcaccc actgacagtc aattccagcc accgttgccg gttcaggcag agtcagacgc
      301 tcgactcgat caaaaatccc aaaccagagc acaggaaatg atcccggagg gtgggttggc
      361 aatgccgggt caaagcctga tgccgcttcg taactcaaaa atccagcggc aaaataccct
      421 tgctcattca ccaactggtc aatggactgc atcaggggca acacgtcatc caccgacgaa
      481 gttgtcagaa tttgcactgg ctgctgaaat cgaagccacg ctcgggtggc agcatcataa
      541 atgacaagcc gaggagtcat gagaattgaa gaattgagaa tgaaaaatga agaatgacct
      601 gaattttgcc ccaaagagca ctacccggct caaagatcag cgccgacccg ttcgagacac
      661 cactgccagg cgcgagtcgg cacgatcagt gagtgaattc gaaggtcacc gaaatcgagc
      721 cttcacggtg accaaaccga cttccaccct gttctttctg tccttttcgt ccttttcgtc
      781 ctttgatttt ggtctcatca ggtaccaaaa ccagggaacg gatcagtatt ttgggcgaaa
      841 tttggggaat gaagcatgaa gagtatcgtt cggagcttat ggcgtgcaag ctcagcgggg
      901 tttatgcctg gggcgttgtt ctgcttcaaa gctcgacctg atcaaaaaat tcattggctg
      961 gcggctcaaa ttggggttgt aatacgggtc gcgttcaagc tgggcgcccc attgacgccg
     1021 catccattca gcgtccgggt cgtggttcaa ccttgtctga cgacttcgga ctggctcggg
     1081 gtcaagcatt cggacctgcg gtacacaaac gtgccgatag ccttcgttga acattcgcag
     1141 acacaggtca acatcccaga atctggcttt taactgtgaa ttgaggccac cactggccac
     1201 aaacacctct ctgcggcagg ccaacccggt cagcggaagc gccgcgacat tgtggctggt
     1261 aatgaggctt ccatgatacc caaaagcagt gccgggcaaa ctctggtgaa gttcagccgc
     1321 gatggtatct cgattgaggc caagtccggc actccgcacc agtcttccag gctggagcaa
     1381 aatcccccca acggcggcga tggcaggccg gtacgcctgt tcaaccagca gttcaagcca
     1441 ctcatcagac tcaaccacca atccggcttt gagaaaaacg atgatctcag tgcctgcctg
     1501 ccggacagcc tgattgagca gttccgggtt gagcgtttgg gaagatggtt caacccggtg
     1561 aactgattgc accgtcgttg gaatcaactt ctttagatat tgatgttgag cctcaaccag
     1621 acaactgacc accgataccg ggctaatcgt ggttttggta aaccacgatt caacactggc
     1681 aaccagttgt tttatatcgg cggttccaaa aacgatgagg ccaactgaca ctgccgcagg
     1741 tcggtcaaac cggatggtgt aatacccagg atgtcgaata ttgatctgga ccgagccagg
     1801 ttcaccacgc cgcgtcaggg cttgactgat ggctttttga gccgccacat aggcataggg
     1861 cttggcctga tagtttaagc tggccgaatc ctggtgcatt cgccagtgat at
//
LOCUS       DAOQDO010001356         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61037_length_1912_cov_2.597738, whole genome shotgun sequence.
ACCESSION   DAOQDO010001356 DAOQDO010000000
VERSION     DAOQDO010001356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61037_length_1912_cov_2.597738"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1912
                     /locus_tag="PKZ53_18810"
     CDS             <1..>1912
                     /locus_tag="PKZ53_18810"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNJ42546.1"
                     /translation="APTVYKTTNGGTSWTSVLQTANNQNVYTGWAGYQGDRGWSFGEY
                     ALGFAVSGSDPNRLIITDFGHPHLSSDGGATWKQAYVDPTTQNEINTPIPKGKSYLSV
                     GMEQTSTWWLTWSDTNNLFAAFTDIRGKRSTDGGNTWSFNYTGHTFNSMYEVIRHPSS
                     GVLYGATSTVHDMYQSTYLTDSRIDGGNGQVLFSTNQGAAWQVAYAAGHPVICVAADP
                     NNANRLYASVIHSTQGGIFVSNNIQNGTSATWTKLTNPPRTQGHPFNIRVLNDGTLVC
                     SYSGRRAGSPLQFQNSSGVFVSTNGGSSWLDRTDQSMIYWTKDVVIDPNDANQNTWYA
                     GVWSGYGTVAPGLPAPQGTGGLYKTTNRGQNWTRILNLDRVTSCTISPTNPNVMYVTT
                     EQDGLWFTNNLNSGSPTFTLVTSFHFRQPERVFFNPFNSNEIWVTSFGSGLWRGTLSA
                     GGTPPSVNNPNPSTICSGGSTTFSVTASGSAPLAYQWQVDTLPINGVYTNVPSSPPYS
                     GTTTDTLTITGATAAQAGNYRVVVSNGVQPDATSQPALLTVDPLATANAGPDQTICSN
                     QTVTLAGNVGGGAASGTWSGGAGTFTPNATTLNAVYTPSAGEVSAGSVTLTLTTNDPS
                     GPCGAATDTITIT"
BASE COUNT          449 a          509 c          512 g          442 t
ORIGIN      
        1 gcaccaactg tgtataaaac caccaatggc ggcacgtcct ggacaagtgt cttgcaaaca
       61 gccaataacc agaatgtgta taccggttgg gccggatatc agggcgaccg tggctggagc
      121 tttggcgagt acgcgcttgg gtttgccgtt tccgggtctg atccaaaccg gctgatcatt
      181 acggatttcg ggcatccgca cctgagcagt gatggtggtg caacctggaa acaagcctat
      241 gttgacccaa ccacgcaaaa tgaaatcaac acacccattc caaaaggaaa atcttattta
      301 agtgtcggaa tggaacagac ttcaacgtgg tggttgacct ggtctgacac caacaatttg
      361 tttgcggcgt ttactgatat tcgtggcaaa cgaagcacgg atggtggtaa cacctggtcg
      421 tttaactata ccgggcacac ctttaactcg atgtatgaag ttattcgcca tccttcgtcg
      481 ggtgttttat acggggcgac ttcaacggtg cacgatatgt accagagcac ctacctgacc
      541 gacagccgga ttgatggtgg caacggacag gtattgttct caaccaacca gggcgctgcc
      601 tggcaggtgg cctatgcggc tggacatcca gtgatttgcg tggcggctga cccaaacaac
      661 gccaatcggc tttatgccag cgtgatccac agcactcagg ggggaatttt tgtttccaac
      721 aacattcaaa atgggacgtc ggctacctgg accaaattga ccaatcctcc gcgaacccag
      781 ggacacccgt tcaatattcg tgttttgaac gatgggactc tggtgtgttc ctactctggc
      841 cgtcgtgccg gaagcccgct ccagtttcag aacagttcgg gtgtgtttgt cagtaccaat
      901 ggtgggagtt cgtggctgga tcgaactgac cagagcatga tctactggac caaagacgtg
      961 gtgattgacc cgaacgatgc gaatcaaaat acctggtacg ctggtgtttg gagcgggtat
     1021 ggaacggttg ctcctggatt gcctgcacct caaggcaccg gcggtttgta taaaaccacc
     1081 aatcgggggc agaactggac gcgaattctg aacctggatc gggtgacttc gtgcacgatt
     1141 agcccaacga atccaaacgt gatgtatgtg accactgaac aagacggttt gtggtttacc
     1201 aacaatttga attctgggtc tcccacattt acactggtca caagtttcca cttccgtcag
     1261 ccagagcgtg ttttctttaa cccatttaat tcaaatgaga tctgggtaac gtcatttggc
     1321 tctggattgt ggcgtggcac actttcggct ggtggaacgc cgccttcggt caacaatcca
     1381 aacccttcga cgatttgctc agggggatcc accaccttct ccgtgaccgc cagtgggagc
     1441 gccccgcttg cctatcaatg gcaggttgac acccttccaa taaacggcgt gtataccaat
     1501 gttccttcat ctccgccgta tagcggcacc acgaccgata ccttgacgat tactggggca
     1561 acggctgctc aggcagggaa ttatcgagtg gtggtcagca atggtgtcca gccagatgcc
     1621 accagccagc ctgctttatt gacagttgat ccactggcga cggccaacgc cgggccggac
     1681 cagacgattt gttccaacca aaccgtgact ttggcgggta atgttggagg tggcgccgca
     1741 tctggcacct ggagcggcgg ggcgggaacc ttcaccccca atgccacaac gttaaatgcg
     1801 gtctacacgc catcagcagg tgaagtttcg gctggaagtg tgaccttgac gctgacgacc
     1861 aatgacccaa gcggtccctg tggcgccgca actgacacca tcacgatcac ca
//
LOCUS       DAOQDO010001357         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61166_length_1910_cov_3.218868, whole genome shotgun sequence.
ACCESSION   DAOQDO010001357 DAOQDO010000000
VERSION     DAOQDO010001357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61166_length_1910_cov_3.218868"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PKZ53_18815"
     CDS             complement(<1..988)
                     /locus_tag="PKZ53_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42547.1"
                     /translation="MYHNWKSHVVSRAIFAAAIVSLNLFIFGITSFSVSAQQPPSQWT
                     SRGAGGGGALFSPSINPSNSSEVYAASDLSALYRTTNYGVSWSVVDFNQIQSFHESQV
                     QFTNDSMIRYCLDFSNDLRTPRKTTDGGVTWQDLTGDPTGGDVYYIAADPNNFNRLIV
                     TDYTTVYFSSNGGASFTAKFSTNVGGAGCHLAGAFFEGANIYLGTNAGLVTSTDNGAN
                     FALASVGGIPAGQAMASFAGAKQGATTRLFCVTYPSGDIFASVTPIGIQADYLGVYRL
                     DVGQANWSLTTTGIAAGTYPYFVGMAQNNISVAYLGGASDANANGDPAPTVYK"
     gene            complement(1344..>1910)
                     /locus_tag="PKZ53_18820"
     CDS             complement(1344..>1910)
                     /locus_tag="PKZ53_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42548.1"
                     /translation="SDGRFHRAIVKNLAYDLALKSDEAGLQCLIEEALQNQSNSLGIS
                     LWSEVEYSVKPLLKTLAVQSNEPSFEKRLIYLFEFLVNPRGIITSDSNTPGCWSFYEL
                     KVFSELWFQNIEIGTQILDRYTQQKPQLRMDFLRLVKDLNIPFMMGTDEEAELYQRVG
                     RIERKWLPLNMPGIPRLKTTPPVQNQQI"
BASE COUNT          506 a          458 c          463 g          483 t
ORIGIN      
        1 ttttatacac agttggtgcc gggtcaccgt tggcattggc atcgctggcg ccgccgagat
       61 aggccactga gatgttgttt tgggccatcc cgacaaaata tggataggta ccagcggcaa
      121 ttccggtggt ggtaagcgac cagttggcct gtcccacatc cagtcggtag acacccagat
      181 aatctgcctg gataccaatc ggcgtcaccg aagcaaaaat atcacctgat ggataagtca
      241 cacaaaacaa gcgggttgtt gccccttgtt ttgctccagc aaacgatgcc atggcctgtc
      301 cggcagggat acctccgaca ctggccaggg caaaattggc gccgttatca gttgacgtca
      361 caagtccggc gtttgtcccc agatagatat tggctccttc aaaaaaggca ccggccaggt
      421 gacaaccggc accgccaacg ttggtggaaa attttgcggt aaacgaagca ccaccattac
      481 ttgagaaata cacagttgtg taatcagtca caatcaggcg attaaaatta tttggatccg
      541 ccgcgatgta atacacatcg ccgccggttg ggtcgcccgt caaatcctgc cacgtcaccc
      601 caccatcagt ggttttccga ggagttcgca aatcattgga aaaatccagg cagtaccgga
      661 tcattgaatc attcgtaaat tgaacctgtg attcgtgaaa cgattgtatt tgattaaagt
      721 caaccactga ccatgacacc ccataattgg ttgttcgata cagggcgctc agatcgctgg
      781 cggcatatac ttcgcttgaa ttactcggat tgatacttgg tgaaaataag gcccctccgc
      841 ctccggctcc ccgtgacgtc cactgggatg gcggttgctg ggcagaaacc gaaaaagaag
      901 tgattccaaa aatgaataga ttcagactga caatggctgc ggcaaagatc gcacggctta
      961 cgacgtgcga tttccaattg tgatacatcc tggcgactcc tggctggtgt gaaaggtacg
     1021 ttgggagtac cttcaaaatc acacacccac ggaaagaact tgagctgagg ctttatcaat
     1081 tggtggtcgg ggtagaaaaa actgacgaag tcccgctgat cagtagtcac acagtttggg
     1141 aggtttcgtc agtgggcgaa tatgagtggt tcgccctaaa aattgcatcc acggttcttt
     1201 ctctaacggg gtgcggtgtg cgggaatccc aatgagtacc tgaggaaggg gtaactggac
     1261 aactacctaa acaggtaggg tgctacaaaa tccagttctt tgccgggaat tgaaagcagt
     1321 tctgttctgg ggctgatact gattcaaatt tgctggttct ggacaggtgg cgttgtcttt
     1381 aatctgggaa taccaggcat attcaacgga agccatttcc tctcgattct cccaaccctc
     1441 tggtacaatt ctgcttcttc atctgtcccc atcataaacg gaatattgag atctttgacc
     1501 aatcgcagaa aatccattct caactgaggt ttttgttgcg tgtagcggtc caggatctga
     1561 gtgccaatct caatgttttg gaaccagagt tctgaaaaaa ccttgagttc ataaaaggac
     1621 cagcaccccg gagtatttga gtcactggtt ataatacctc gtgggtttac caaaaactca
     1681 aacaggtaaa tcaatctttt ttcaaaagac ggctcattgc tttgaaccgc caaagttttg
     1741 agaagcggct ttacagaata ttcaacttca ctccaaaggg agattcctaa actgttgctc
     1801 tgattttgca gggcttcttc aattaaacac tgcaaaccag cttcatccga tttgagggcc
     1861 aggtcatagg ccaggttttt gacaattgcc cggtgaaatc ggccatcact
//
LOCUS       DAOQDO010001358         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61180_length_1910_cov_2.761725, whole genome shotgun sequence.
ACCESSION   DAOQDO010001358 DAOQDO010000000
VERSION     DAOQDO010001358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61180_length_1910_cov_2.761725"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1781)
                     /locus_tag="PKZ53_18825"
     CDS             complement(342..1781)
                     /locus_tag="PKZ53_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42549.1"
                     /translation="MKITYACNSKLPRLAWCARLGKNADVIEVLHGSWVETGETGFFE
                     GAWDDEFETFRFDDAQMAVGSGARLTTDGIVFVAPGHSLEWIQSLRIEDELLISNSLP
                     FLLMQARDGLDFGHPFYLFDFLNRYRQGLAVHTKTIPTAQQRQVQIHDGCNLLVNTDL
                     TVQRLEKPHPSPPGNYHEYVQLLQETLNRVSANAQDRQRQRHYPPVTTISTGYDSTAI
                     SVLASQSGCREALTFRQSGDLNQGYLHDSGAHIAHLLGLNVTEFDREAIQNLPFLTDA
                     EFFINPLISTDRGFLVFEDHLTSRLVLTGRNGENYWTTDQKLIFPLLQEPSARLMSGT
                     TLGEYRLRAGFIHFPVPFCGAVHAETIFQITMSDDLKPWSIGGEYDRPIARRIIEEAG
                     IPRQLFGQVKKGGGNKARPYRLTNRCEADFQAFYKSEIQPRFPQPNFDQLESAARYAE
                     RDYTHRNWNSPNLFVFHWGFERIKYRYQV"
BASE COUNT          503 a          458 c          493 g          456 t
ORIGIN      
        1 aaaaaagctt tggaaaaacg tccacctgca ctctggtcta aattacgaac caaccgatcc
       61 accacgtcag ttcctcgcgc atcattcaag tctggaatat agagtgtcgc gagttcttca
      121 gcagttaaag gctcattcag aaagaattta tacacttcat tgagagtttg tgccggcata
      181 gggtgactcc aggcaatggg gtaatagagc agggcgaatc agggttcaga gttcagggaa
      241 atacaattct ttcaatgatt tagctttgat atagcacaag ggggttattg caaagtggta
      301 taccctcttt gaagaggaga atgcaagcca aagtcgaatg actacacctg ataccggtat
      361 ttgattcgct caaagcccca atggaagaca aacaggttcg gcgagttcca gttccggtga
      421 gtgtagtcgc gctccgcgta acgggcggcg ctttcaagct gatcaaagtt gggttgtggg
      481 aagcgtggct gaatttctga tttgtaaaat gcctggaaat ccgcttcgca acggttggtg
      541 agccggtagg gacgagcttt gttgccaccg ccttttttaa cctgaccgaa caactggcgc
      601 gggatgccgg cttcttcaat gattcggcgg gcaattgggc ggtcatactc accgccaatg
      661 gaccagggtt tcaggtcatc cgacattgta atttgaaaaa tggtttcggc gtgaaccgca
      721 ccgcaaaacg gcacggggaa gtgaataaac ccggcccgca accgatattc acccagggtt
      781 gtgcctgaca ttaagcgggc agatggttcc tggagcaagg gaaaaatcag cttctggtcg
      841 gtggtccagt agttttctcc gttgcggccc gtcaaaacga gccggctggt cagatggtcc
      901 tcaaaaacca ggaacccacg gtcagttgaa atcaatgggt tgataaaaaa ttcggcgtcg
      961 gtcaagaaag gcagattttg gatggcttcg cggtcaaatt ctgtcacgtt cagtcccagc
     1021 aggtgggcaa tgtgcgcgcc gctgtcgtgc aggtagcctt ggttgaggtc accgctttga
     1081 cgaaaagtga gtgcttcacg gcaaccactc tggctggcca gaaccgagat cgcagttgaa
     1141 tcatacccgg tcgagatcgt agtcaccgga ggatagtgcc gctgcctttg tctgtcctga
     1201 gcattggctg acacgcggtt gagggtttcc tggagcaact gaacatactc gtgatagttt
     1261 cctggcggag atggatgcgg tttttcaagc cgctgtactg tcaaatctgt gttcaccagc
     1321 aggttacagc cgtcgtgaat ctgaacctgt cgctgctgtg ccgtcggaat ggttttggta
     1381 tgaactgcca gtccttgccg ataccggttt aaaaagtcaa acaggtaaaa cggatgccca
     1441 aaatcaagtc cgtctctggc ctgcatcaac aaaaacggca gcgaattgga aatcaggagt
     1501 tcgtcttcga ttcgaagtga ttgaatccat tcgagactgt gacctggcgc cacaaaaaca
     1561 atgccatctg tggtcaatcg tgccccggaa ccaacggcca tctgggcgtc atcaaatcga
     1621 aaggtctcaa actcatcatc ccaggcgcct tcgaaaaatc cagtttcacc tgtttcaacc
     1681 cacgatccat gcagaacctc aatcacatcc gcatttttgc cgagtcgcgc acaccacgcg
     1741 aggcgtggga gctttgagtt acaggcatag gtgattttca tggaatatgt accttaccaa
     1801 agattccaag acattattaa ccacgaaaac cacgaaaaac acgaaaagaa tcaaatgctt
     1861 accagattca atctctcagt caacttatga caaggtactt ataatgagtc
//
LOCUS       DAOQDO010001359         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61183_length_1910_cov_2.692183, whole genome shotgun sequence.
ACCESSION   DAOQDO010001359 DAOQDO010000000
VERSION     DAOQDO010001359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61183_length_1910_cov_2.692183"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1910)
                     /locus_tag="PKZ53_18830"
     CDS             complement(<1..>1910)
                     /locus_tag="PKZ53_18830"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ42550.1"
                     /translation="SSFFILHSSFFILHSSFCLLAQPQSITAQVEGKPPDIAEYGYPT
                     FRTYTDRDGLPQNTINQVTTDRKGYLWIGTQNGAAFFNGHRWTVVNMQGGTLSNRVRS
                     LLQAADGSYWFGTEDSGLLRKDGDNWVAFSRLSGALLSDEVKCMVETVTPTTAKSTLW
                     VGTSAGLSCFESGKWTHFTAEDGTLPNNRVMCLLQTTGTNGAPVVWAGTEGGGLVKFE
                     AGAWTRLDTSAIASNAVLSLMATVSPKTQEQTLWVGTSGGLARVEQGRWTLYDINTGL
                     PSNVVLSLLETRLPDGTPTVWVGTYGGGLARFEQNRWITFNTKNSTLPNNGVRSLIET
                     RSETGTRLLWVGTYGGGLARFEQSQWIGFDTKTGLPNDLVYSFLESKNPTGEYGLWLG
                     TGAGLVYYLKGEIETYTIAPGKLNSDVIYSLLETRTPEGVPILWIGTYGGGLSRFDGK
                     NWQTFIAPDLPSNIIYCLYETRTPAGNSVLWVGTDNGLARLEDGVWQTFQMDAGLPDN
                     VIYGLFQTTSSRGISTLWVATRNGLAAFQNGQWTSFGSETGLPKTAMYSFLETSAPGQ
                     PPQLWIGTGGAGVYTLDLTGWYGTDSLKTVRPLPVEIRGTVFRMLATPKGAVYLTTNT
                     GVVRLEPATTGSG"
BASE COUNT          468 a          541 c          471 g          430 t
ORIGIN      
        1 ccggaaccag tcgtggctgg ttctaaccgc acaacgcccg tgttggtggt caaatacaca
       61 gcaccttttg gcgttgcgag cattcgaaaa accgtccctc gaatttcaac cggcaacggg
      121 cgaaccgtct tcagcgaatc ggtcccatac cacccggtta aatcgagggt gtagacgcca
      181 gcccctccgg ttccaatcca taactgcggt ggctggcctg gggctgaggt ttcaaggaaa
      241 ctgtacatgg ctgttttggg gagaccggtt tcagaaccaa aactggtcca ctgcccattt
      301 tgaaaagccg ccaggccatt gcgcgtggcc acccacagcg ttgaaatccc cctactggat
      361 gtcgtttgaa acaagccata aatgacattg tcaggcagcc cggcatccat ctgaaaggtt
      421 tgccagacac catcctccaa tctggccaat ccattatcgg taccaaccca cagcaccgaa
      481 ttcccggctg gtgtccgggt ttcatagagg caataaataa tgttgctcgg cagatcaggc
      541 gcaataaagg tttgccagtt tttcccgtca aagcgtgaga gtccgccgcc ataggttcct
      601 atccataaga tcggaactcc ttcaggtgtg cgagtttcca acagcgaata aatcacgtcc
      661 gagtttaatt tgcctggagc aatcgtatag gtctcaattt cccccttcag gtaatagacc
      721 agcccggcac cagtccccaa ccacaaacca tattcaccag ttgggttttt tgattcaaga
      781 aatgaataca ccagatcatt gggaagaccg gttttggtat caaacccaat ccactggctt
      841 tgttcaaacc gggccagccc tccaccatag gttcccaccc acaacagacg agttccagtt
      901 tccgatctgg tttcgatcag gctccgcacg ccgttattgg gtaaggtact gtttttggtg
      961 ttaaacgtaa tccaccggtt ttgctcaaag cgggccaatc ctccaccata ggttccaacc
     1021 caaacagttg gcgtgccatc tgggagcctg gtttcaagca agctcaaaac cacattgctg
     1081 ggtaacccgg tgttgatgtc atagagggtc caccggcctt gttcaacccg agccagcccg
     1141 ccagaagtcc ccacccaaag cgtttgctcc tgggtcttgg gggaaacagt cgccattaac
     1201 gacaaaacgg cgttgctggc aatcgcactg gtatcgagcc gggtccacgc tccggcttca
     1261 aacttgacca gtccaccacc ttcggttcca gcccaaacaa cgggtgcccc attggtcccg
     1321 gtggtttgga gcaggcacat aacccggtta ttgggcaagg ttccatcttc cgccgtaaag
     1381 tgggtccact ttccgctttc aaaacacgag aggccagccg aagtcccaac ccagagcgta
     1441 cttttggcgg tggtcggtgt gaccgtctcg accatgcact tgacctcatc tgacagcagg
     1501 gccccgctca gacgggaaaa ggcgacccaa ttatcgccat ccttgcgcag caaaccacta
     1561 tcttcagttc caaaccagta acttccatcc gccgcctgga gcagtgatcg aacccggttt
     1621 gacaatgttc cgccctgcat attgaccacc gtccagcgat gaccattaaa aaaggcagcc
     1681 ccattctggg taccgatcca aagatagcct ttgcgatctg tcgtgacctg gttgatggtg
     1741 ttttgtggga gtccgtcacg atcagtgtag gtgcgaaagg tgggatagcc atactcagca
     1801 atatctggcg gctttccttc gacctgagct gtgatggatt ggggttgagc cagcaagcag
     1861 aatgaagaat gaagaatgaa gaatgaagaa tgaagaatga agaatgaaga
//
LOCUS       DAOQDO010001360         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61221_length_1910_cov_1.722911, whole genome shotgun sequence.
ACCESSION   DAOQDO010001360 DAOQDO010000000
VERSION     DAOQDO010001360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61221_length_1910_cov_1.722911"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKZ53_18835"
     CDS             <1..211
                     /locus_tag="PKZ53_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42551.1"
                     /translation="YFQSPTSVWITYGQTAIYNPTRWSMFTQDWRQKLVRAALIDSKT
                     QKLMGLAGGMANIPHLSDWSFVNTH"
     gene            289..786
                     /locus_tag="PKZ53_18840"
     CDS             289..786
                     /locus_tag="PKZ53_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42552.1"
                     /translation="MVRTLITDEHGTSSIELMLLLPLALGAIAFMVWVGQIMLVRQRT
                     MVAARFAVEHTIATGGQPTSSEIARAVTTPGKDWHLQTRTSSGTGQLTENSNEGLITA
                     AASTTPPQPPILNNQPFRLTRGTYATLNGTWTCDSQGDTSIFSLITVSFLPIPTPCCE
                     SYPSR"
     gene            855..1655
                     /locus_tag="PKZ53_18845"
     CDS             855..1655
                     /locus_tag="PKZ53_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42553.1"
                     /translation="MGCSVTEQSGPVPRAIPGDAGNGSPVQPIANPESAVSQPRADPS
                     VSGSDSTEGTAPESPQAYAKSISAWYLEQGFEQVELDIEQGIAPSTQLLDGKHFWVTQ
                     RQGTWFVQAVEGNGTQGVYTLTVIESGPAGSTRWTRHRTESSADSASPVPQLSVPDQN
                     ATALPADVPVHPSLSALVTPIQTNQAADEMFAVFESPETVTSAELTQWFQTEMKRFGW
                     ESTPLPAESEPGMAAFLKGTTMCLIIISPNPDLRTSSVAISLRTVESP"
BASE COUNT          490 a          510 c          458 g          452 t
ORIGIN      
        1 gtactttcaa tctccaacca gtgtctggat tacctatggc caaactgcca tctacaaccc
       61 aacacgctgg agcatgttta cccaggattg gcggcaaaaa ctggttcggg cagcgttgat
      121 tgattccaaa acccaaaaac tgatgggcct cgcgggtggg atggccaata ttccccatct
      181 ctcagactgg tcgtttgtca acactcatta gtcaatcacg caaataaagg ggggagcgcc
      241 aaaactgctt cacccccttc atttacatta acaaaaggaa gcacggttat ggttcggaca
      301 ttgatcactg acgagcacgg tacctccagc attgaactca tgttattact gccactggct
      361 cttggcgcta tcgcgtttat ggtctgggtg ggccaaataa tgctggttcg gcaacggaca
      421 atggtggcgg ctcgatttgc agttgaacat accattgcga ccggagggca acccacttcg
      481 tcagaaattg cacgggcggt tacgacacca ggaaaggact ggcacctgca aacccgtacc
      541 agttctggaa ctggccagtt aacggagaat tcaaacgaag ggttgattac cgccgctgcg
      601 tcaaccacgc cgcctcagcc gccaattttg aataatcaac cgttcaggtt aactcgtgga
      661 acatatgcca ccttgaatgg cacctggacc tgtgacagtc agggcgacac gtcaattttt
      721 tcattgatta cagtctcttt tcttccgatt ccaacccctt gttgcgagtc gtatccatca
      781 cggtgaatcc aatgcacaaa atacaatcag tgtggtgctc cattttttgg gtttgttttc
      841 ttggcttgat cacggtgggt tgttcggtca ctgaacaatc cggcccggta cctcgtgcga
      901 tacctggtga tgctggcaac ggatcgcccg ttcagccgat tgccaatcct gaatctgctg
      961 tcagtcaacc cagggctgat ccatcggtct cgggctcaga ttcaaccgaa ggtacagcgc
     1021 cggaatcacc ccaggcatat gccaaatcca tcagtgcctg gtaccttgaa caagggtttg
     1081 aacaggtgga actcgatata gaacaaggaa ttgccccgtc cacacaactg ttggacggaa
     1141 aacacttttg ggtaacccaa cgccagggca cctggtttgt ccaggcagtc gaaggtaatg
     1201 gtacccaggg ggtgtatacc ctgacagtca ttgaatcagg gccagccggt tcaaccaggt
     1261 ggaccagaca ccgtactgag agttcagcgg actcggcttc acctgttcca caactctcag
     1321 tacctgacca gaatgcaact gccttaccgg ctgatgttcc agtgcacccg tcattgtccg
     1381 cactggtgac cccgattcaa accaatcaag ctgccgatga aatgtttgcc gtttttgagt
     1441 caccagagac tgtcacctca gccgaactca ctcagtggtt tcaaaccgag atgaagcgct
     1501 ttggttggga atcaacaccg ctgccagccg agagtgaacc tgggatggcg gcatttttaa
     1561 aagggacaac catgtgcctg attatcattt ccccaaatcc agatcttcgc acaagcagtg
     1621 tggccatcag tctgagaacg gttgagtctc cataatctgc tggcaaccag ggtcagttat
     1681 actcaacata ggcttaattt gggattttta ttttcaccac agagggcaca gaggagcaca
     1741 gaggaaatac aaaccaagac tccattctct ctgtgtcctc tgtggtgaaa aaatcacacc
     1801 ttcacacaaa ggagacgcac atcattatgg accagacaca gtcccgtatt cggctgcaga
     1861 gcccatcaaa gaagcaaaaa gtactgataa ttgcggcgat tctggcctgt
//
LOCUS       DAOQDO010001361         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61249_length_1909_cov_3.269687, whole genome shotgun sequence.
ACCESSION   DAOQDO010001361 DAOQDO010000000
VERSION     DAOQDO010001361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61249_length_1909_cov_3.269687"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1183)
                     /locus_tag="PKZ53_18850"
     CDS             complement(5..1183)
                     /locus_tag="PKZ53_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42554.1"
                     /translation="MTLTLICSFSQLAVAFFQIPFPKPKLPKSPSESSKPQPTPPNQT
                     PARPERPVTNGSGSPVPPQPAPIGPTLLKMSLDVRADTMSRYWKLPNESFYSSWVPQL
                     KFQIQFVGSPNPQYTVEFTGKDGKPWFAEDCKTNRSGQTVVDVWTPGAKQPSDERSTI
                     DTGTYGFTIKHKETGETIYQGKYKVTKFHYGPGIPMYKNQHAYVVDNDWNLPIGYLWL
                     DTTGNDRYAPKLKFATWFRGEARADNVEGKLFYNGQEIATTKNKGEALSKVARFSTLE
                     DKPNTRYERWELGWYLVLGYQDAKSGFKYPGAHILDKNPGSYEVKVLRDGVIVREVQF
                     TVGPDGHIVDNGLAKQNNLGDDRMMVPLKVLGTLDGTWNQASWQADAFYGNPVAGLSK
                     "
     gene            1720..>1909
                     /locus_tag="PKZ53_18855"
     CDS             1720..>1909
                     /locus_tag="PKZ53_18855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ42555.1"
                     /translation="MPHQKRFSSSTTPQSEQGLSLLELLVTLFLLTLFVGVLVTGITV
                     YQKYRVRVATQKRLQELTN"
BASE COUNT          464 a          451 c          460 g          534 t
ORIGIN      
        1 acccctattt tgaaagacca gccaccgggt tgccataaaa agcatcggcc tgccaggaag
       61 cctgattcca ggtgccatca agcgtgccca ggactttcag cggaaccatc atccggtcat
      121 cacccaggtt gttttgtttg gccaatccgt tatcaacgat atggccatct gggccaactg
      181 tgaattggac ttcccggaca atgaccccat cccgtaaaac tttgacttca tagctgcctg
      241 ggtttttatc gaggatgtgg gcgcccggat atttgaatcc acttttggca tcctggtatc
      301 caagcacaag ataccagccc aattcccacc gttcatagcg cgtattgggt ttatcttcga
      361 gtgtgctaaa gcgtgccact tttgagaggg cttcaccttt gttttttgtt gtggcaattt
      421 cctgaccatt ataaaacaac ttgccttcga cattgtcggc tcgggcttca ccacggaacc
      481 acgtggcaaa tttcagtttg ggggcatagc ggtcattgcc ggttgtgtcg agccagagat
      541 atccaatcgg caaattccag tcattgtcaa ccacgtaggc gtgctggttt ttatacattg
      601 gaatacctgg gccgtaatga aatttggtca ctttatactt tccctgatag atcgtttcgc
      661 ctgtctcctt gtgcttgatg gtaaatccat aggtaccagt atcaatggtt gaacgctcat
      721 ccgatggttg tttggcgcct ggagtccaca catccaccac ggtttggcca gagcggttgg
      781 ttttgcaatc ttcagcaaac cagggctttc catctttgcc ggtaaattca acggtgtatt
      841 gaggattggg agaaccaaca aactgaatct gaaatttgag ttgcggcacc cagcttgagt
      901 aaaacgattc attgggcaat ttccagtacc gcgacatggt gtcagcccgg acatcgagtg
      961 acattttcaa caacgttggg ccgattgggg ctggttgcgg aggcaccgga gaccccgacc
     1021 cgttcgtcac cggacgctcg ggtcgagctg gagtctggtt tggcggtgtg ggttgtggtt
     1081 tagaagattc tgacggcgat ttcggcagtt ttggtttggg aaacggaatt tggaaaaagg
     1141 caactgcgag ttgagaaaaa ctacaaatca gcgtcagtgt catgaagctt tgaataagaa
     1201 aagaccagcg taatttcatg ggctcttgcc tccacagtgt tgaatcagca cacaagattt
     1261 gatgtattac cgttcacaac ggagaaccag agggcatttg gtcaaccagt gagatttaat
     1321 tccaagatta aatttaaatt gatttttcca gctcgtttaa atgcatttat ttcaataaac
     1381 actttgattt tcggcaattg aaaatctgat tgacttttgg attcatccag gcacaatatc
     1441 tggtcactgt gtgggggcca cctgcactca attccacgac ctgacttctc tgatacacat
     1501 cagcccctga gcggccaacc tcaagcggag gatcaagttc cgaaaccggg cttatatttt
     1561 ccgccaaacc ggaaaagccg ccgattttat attttcgcct gcctggatca aacggatcaa
     1621 atgactcaat ggaaatgatt ctctaaaaga tgatccgctt tggcacggtt tttgttttat
     1681 cggagtgcag tttaaatttt ttgcaggatg actcttttta tgccgcatca gaagcgattc
     1741 tcatcatcca ccacccccca gagtgaacag gggttgagtt tgctcgaact gcttgtcaca
     1801 ttatttctgc tcacgctttt tgtcggggtc ctcgtcacgg ggattacggt gtatcaaaaa
     1861 taccgtgttc gggtggcgac tcaaaaacgg ctgcaggaac tgaccaacg
//
LOCUS       DAOQDO010001362         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61261_length_1909_cov_2.720065, whole genome shotgun sequence.
ACCESSION   DAOQDO010001362 DAOQDO010000000
VERSION     DAOQDO010001362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61261_length_1909_cov_2.720065"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKZ53_18860"
     CDS             complement(<1..225)
                     /locus_tag="PKZ53_18860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42556.1"
                     /translation="MINGLKVVVVLPAYNAAKTLLKTYNEVDRNVVDELILVDDASHD
                     DTAEVARNLGIKTIVHTTNLGYGGNQKTCYR"
     gene            complement(391..1296)
                     /gene="hemF"
                     /locus_tag="PKZ53_18865"
     CDS             complement(391..1296)
                     /gene="hemF"
                     /locus_tag="PKZ53_18865"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNJ42557.1"
                     /translation="MANTTQHPMLDRAREFFAALQDEICAALEAVDGSAFQQDLWERP
                     GGGGGRTRVLQEGSVFEKGGVNFSYVFGEMPEKLARQVDSGGDRSFSATGVSLVLHPR
                     NPMVPTVHANFRYFEQGSVAWFGGGADLTPYYPYREDAVHFHTVLKAACDQHHPDYYP
                     RFKKWCDDYFYLPHRNETRGVGGLFFDHLKGEESSLEAIFAFVQDAGKAFLPAYLPIV
                     ERRQPEPYAERERTHQLIRRGRYVEFNLVYDRGTLFGLETKGRTESILMSLPPLTKWV
                     YDYRPEPGTPEAAALEFYQPHNWLD"
     gene            complement(1406..1816)
                     /locus_tag="PKZ53_18870"
     CDS             complement(1406..1816)
                     /locus_tag="PKZ53_18870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ42558.1"
                     /translation="MAVEITGIYTGNLKFELTHGPSGTVIRTAPPVDNNGDGSSFSPT
                     DLASAALGACMVTIVAMVAERDGIDCTGLGFHVEKHMSTTAPRRIGRLPVRITMPKGL
                     TDEQKAKLERAALTCPVHHSLHPDIEKPVTFIYE"
BASE COUNT          460 a          475 c          500 g          474 t
ORIGIN      
        1 gcgataacag gttttctgat taccgccgta gccaaggttt gtagtgtgaa caatggtttt
       61 aatcccaaga ttccgtgcaa cttcagcagt gtcatcgtgg ctggcgtcat caaccagaat
      121 cagctcatca accacgttgc gatcaacttc attataagtt tttagcagtg ttttggctgc
      181 attataagct ggtaaaacga caacgacttt gagtccatta atcataatta cagggcttgg
      241 ggcttggggc ttggggttta aggcagacaa agtgacaaag gaaagaatga caagagggta
      301 aagtgacagg gtgatttttt tcaccgatca tcttttctga aaatccggca cctggtcagt
      361 tttcttcatt cttcattctc aattcttcat tcaatcgagc cagttgtgcg gttgataaaa
      421 ttcgagggcg gcggcttcag gggttccagg ttcaggccgg taatcataca cccattttgt
      481 gagtggtggg agcgacatca aaatagattc ggttcgacct ttggtttcaa ggccaaacaa
      541 ggtgccgcga tcatagacca gattgaattc gacataccga ccacgacgaa ttaactgatg
      601 ggtgcgctcg cgttcagcat aaggttccgg ctggcgacgt tcaacaatcg gtaaataggc
      661 agggagaaat gctttaccgg catcctgaac aaaagcgaaa atcgcctcaa gactgctttc
      721 ttcacccttc aagtgatcaa aaaacaaacc gccgacacca cgggtctcat tgcgatgggg
      781 caggtaaaaa tagtcatcac accatttctt aaagcgcgga taatagtcgg ggtggtgctg
      841 gtcgcaagcg gccttcaaga cggtgtgaaa gtggacggcg tcctcgcgat acggatagta
      901 aggcgttaaa tcagcccctc cgccaaacca ggcgacgctt ccctgctcaa aatagcggaa
      961 attggcatgc acggttggca ccattgggtt tcgcggatgc aggaccagtg acacaccagt
     1021 ggctgaaaac gaccggtctc ccccgctgtc tacctgccgg gccagttttt caggcatttc
     1081 gccaaacaca taggaaaaat tcacgccgcc cttttcaaaa acactgcctt cctggagcac
     1141 gcgggttcgt ccgccgccgc cgccgggacg ctcccataaa tcctgttgaa aggcgctgcc
     1201 gtcaaccgcc tccagcgcgg cgcaaatttc atcctgcagg gccgcaaaaa actcgcgtgc
     1261 ccgatcaagc atggggtgtt gggtcgtgtt cgccattgtg tcttctcggt taaagatgcg
     1321 ggttcagggt tcaggatatc gagaaaatac cagagccata aagaaccaca atttgaaaac
     1381 cctgaaccct gaaccctcaa tagtactatt catagataaa ggtaactggc ttttcgatat
     1441 ctggatggag gctgtgatgg accgggcagg tcaacgccgc ccgttcaagt ttagcttttt
     1501 gctcatctgt cagtcctttg ggcatggtga ttcgcaccgg taaccggcca atccgacgcg
     1561 gtgccgtggt ggacatgtgt ttttcaacgt gaaagccaag tccagtgcag tcaatgccgt
     1621 cacgttcggc gaccatcgcc acaatcgtca ccatacaggc cccgagcgcg gctgaagcca
     1681 ggtcagttgg tgaaaacgaa ctgccatcgc cgttattgtc aaccggcggc gccgtgcgaa
     1741 tgaccgttcc tgacggtccg tgcgtgagtt caaacttcaa attaccagtg taaatgcctg
     1801 tgatttcaac ggccatggga aagtctccgg gttcttggtg cttggtactt ggtgcttggt
     1861 gcttggttct tggttcttgg ttcttggtgc ttggttcttg gtgcttggt
//
LOCUS       DAOQDO010001363         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61310_length_1908_cov_2.582299, whole genome shotgun sequence.
ACCESSION   DAOQDO010001363 DAOQDO010000000
VERSION     DAOQDO010001363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61310_length_1908_cov_2.582299"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKZ53_18875"
     CDS             <1..295
                     /locus_tag="PKZ53_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42559.1"
                     /translation="AAVDRETQPGDTTPAESLPPPRPKIRIKPKNQSTHIQPDMGSPA
                     QPESVTVVPNSESGLGNSQLIRAAVPSTVKASDKKKPKSKAPTSTGQMSLFDL"
     gene            complement(339..1142)
                     /locus_tag="PKZ53_18880"
     CDS             complement(339..1142)
                     /locus_tag="PKZ53_18880"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNJ42560.1"
                     /translation="MSQPDYERLLQLIADQATEGLDLEGQKELQDLMSHYPQSDPTEV
                     ELAAAAALLASIQIDEPLPAHLQRQILAKSEEFFASTSQPVAAQVIPLVRPRLAGWQW
                     AGWYAAAASLALALFLTWVHLRPQPLIPSSQPLAAKDVRELLIQKNADTLVLPWQGVM
                     LSADVKVSGDVVWNTDVQKGFMRFQGLPVNDPKVSVYQLWIFDATRDDRFPVDGGVFN
                     VDSTTGEVLVQIDPKLYVNKPTLFAVTIEPPGGVVVSKRDKLILAARVT"
     gene            complement(1139..1708)
                     /locus_tag="PKZ53_18885"
     CDS             complement(1139..1708)
                     /locus_tag="PKZ53_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ42561.1"
                     /translation="MTGSILKRIASGDKAAVEECIATFGGLIWSLARRFTYTQADAED
                     AVQDIFIDLWKNAARYDPAVAMEVTFVTMIARRRLIDRLRQNRRHNDTEAITDELSNF
                     LADPKRPDREVELCADAATAVKAMEQLRPEQQKIIQLAVFHGMTHQEIADLTGTPLGT
                     VKSHVRRGLLHIREALGVKVTTVAKESSS"
BASE COUNT          478 a          480 c          482 g          468 t
ORIGIN      
        1 agcagccgtg gaccgtgaaa ctcagccagg agataccaca ccagcagagt cattgccacc
       61 gcctcgtcca aaaatcagga taaaaccgaa aaatcagtca actcacattc aacctgatat
      121 gggatcacct gctcagccag agtcagtcac agtggttcca aactcagaat ctggcctggg
      181 caactcacag ttgatccggg ctgcggtccc ctcaacggta aaagcttcgg acaaaaagaa
      241 gccaaaatcc aaagccccta ccagtactgg ccagatgtca ttgtttgatc tgtaactata
      301 gaatcgaatt ggcgctcgca ggtgggctga tcgtttattt aagtaaccct ggctgccagg
      361 atgagtttat cccgttttga gaccaccact ccgccaggag gttcaatcgt gacagcaaac
      421 aacgtcggct tattgacata cagtttggga tcaatttgga ccaggacttc gccagtggtt
      481 gagtcaacgt taaaaacgcc tccgtcaacc ggaaaccggt catcgcgtgt ggcatcaaag
      541 atccagagtt gatacaccga aactttggga tcattgaccg gtaatccctg aaaccgcata
      601 aaccctttct ggacatcagt attccagacg acatcacctg aaactttgac atcagccgaa
      661 agcatgacac cttgccaggg caagaccagg gtatcggcgt tcttctgaat caacaactcg
      721 cgaacgtctt tggcagcaag tggttgtgac gaagggatga gtggttgtgg gcgcaaatgc
      781 acccaggtga gaaacaaagc cagggccaag ctggccgctg ccgcatacca tcctgcccat
      841 tgccaccctg ccagcctggg tcgaaccagg ggaatcacct gggccgccac gggttgggag
      901 gtactggcaa aaaattcttc agattttgcc aggatttgcc gctgtaaatg ggctggaagc
      961 ggttcgtcga tctgaattga agccaacaaa gccgctgcgg ctgcaagttc cacctctgtc
     1021 gggtcggatt gtggatagtg agacatgagg tcttgcaatt ctttttgccc ttccagatca
     1081 agcccttcag ttgcttgatc agctatcaat tgtaacagtc gttcgtaatc tggttggctc
     1141 atgacgacga ttcctttgca acagtggtaa ctttaacccc gagtgcttca cgaatgtgga
     1201 gcagaccacg gcgaacgtgc gattttaccg tgccgagtgg cgttccggtt aaatcggcaa
     1261 tttcctggtg agtcattccg tgaaaaacgg ccagttggat gattttttgt tgctcgggtc
     1321 ggagttgttc cattgctttg acggcggttg ccgcatcggc acacaattcc acttcccggt
     1381 caggccgttt tggatctgcc aaaaaattgc tcagttcgtc ggtgatggct tcggtgtcat
     1441 tgtgccgccg gttttggcgc agtcggtcaa tcaatcgccg ccgggcaatc atggtgacaa
     1501 aggtcacttc catggccact gccggatcat aacgggctgc atttttccaa aggtcaataa
     1561 atatatcttg cacggcgtct tccgcatccg cctgggtata cgtaaagcgc cgggccagac
     1621 tccaaatcag gccgccgaac gtagcaatac attcttcgac agccgcttta tctccagagg
     1681 caattcgctt gaggatcgat cctgtcacgc gcgtctcttc ctttatggaa tcaaattcat
     1741 acaagtttgt agtcagtagt cagtagtcag tagtcagcag tcagtagttc actaactcta
     1801 ttttgttgaa tcacttgacc ggtttttaat gggaaggctt cattgcaaat tggtataaga
     1861 atgaagaatg agtggtgaat gatgagccag gggatacaaa tttgggat
//
LOCUS       DAOQDO010001364         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61346_length_1907_cov_3.781317, whole genome shotgun sequence.
ACCESSION   DAOQDO010001364 DAOQDO010000000
VERSION     DAOQDO010001364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61346_length_1907_cov_3.781317"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKZ53_18890"
     CDS             complement(<1..333)
                     /locus_tag="PKZ53_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNJ42562.1"
                     /translation="MALEGTAHVYIRDHINTDEIIPARYLNTSDEPTLARHAMEDIDP
                     AFTGRVQKGDIIVAGENFGCGSSREHAVWALRGAGVSAVIASSFSRIFYRNAINNGFL
                     IIECPEASA"
     gene            complement(434..1756)
                     /locus_tag="PKZ53_18895"
     CDS             complement(434..1756)
                     /locus_tag="PKZ53_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNJ42563.1"
                     /translation="MPAQPQTITEKLLAAHCGRETVHPGEIVNAEIDLVVAHEVTTPP
                     AIKMLAKLGITKVFDPKRIVVTPDHFVPNKDIQTAQLTKELREWAIDQGIERYYELGN
                     HGICHALIPEKGHILPGMTAVCADSHTTTLGAFGAFATGIGSTDLAAVLATGELWFKV
                     PETLRFELEGELPEGVYAKDLMLYIISKIGVDGARYAAMEFTGSTIDGLSMEGRMTLC
                     NMAIEAGGKTGIIAADSTTKAYLAERGQTEFKIFASDLDATFAQNIRIQADELEPIVA
                     LPHLPSNGRFIGDVEPMGIKVDQAYIGSCTNARIEDLRIAAQILKGRTVARGTRTMVV
                     PATSDIWRQALREGLLEIFADAGCVVSTATCGACLGGHMGVLGAGEKCISSTNRNFVG
                     RMGSPKSEVYLASPATVAASALTGVITDPRPFLNEELRMKNEEFAMKN"
BASE COUNT          454 a          502 c          509 g          442 t
ORIGIN      
        1 ggcgctggct tcggggcatt cgataatcag aaaaccattg ttgatggcgt tgcgataaaa
       61 aatccgtgaa aaactggagg caatcacggc tgaaacccca gcaccacgta gcgcccagac
      121 ggcgtgttca cgcgaggaac cacagccgaa attctcgcca gccacaatga tgtcaccttt
      181 ttgcacccgg cctgtaaacg ccgggtcaat gtcttccatc gcgtggcggg ccagggttgg
      241 ttcgtcgctg gtgtttaaat accgcgccgg aataatctcg tcggtgttga tgtgatcgcg
      301 aatgtaaacg tgagcagtgc cttcaagtgc cataactgta gggttcaggg ttcagggttc
      361 agggtttgag aatgaagaat gtagaatgaa gaatgtagaa attaagtggc tgaaacctca
      421 ttgttcttca ttctcaattt ttcatcgcga attcttcatt cttcattctc aattcttcat
      481 tcaagaaagg acgtgggtca gtaatcacgc ccgtcagtgc cgaagccgcg acagtcgcag
      541 gactggccag atagacttcg cttttcgggc tgcccatccg accgacaaaa tttcgattgg
      601 tgctggagat acatttttca ccggcgccga gcacgcccat atgaccgccc agacaggcac
      661 cacaggtcgc agtcgagacg acacagccag catcagcaaa aatttcaagc aatccttcgc
      721 gcagggcctg ccgccagatg tcgctggtgg ccgggaccac catcgtgcgg gtgcctctgg
      781 caacggtgcg gcctttgaga atctgtgccg caatccggag gtcttcaatc cgggcgttgg
      841 tacaggaacc gatatatgcc tgatcaactt tgatccccat cggctcgacg tcaccgataa
      901 accgaccatt gcttggcaga tgtggtaggg ccacaatggg ttccagttcg tcggcctgaa
      961 tccgaatgtt ttgagcaaaa gtggcatcca ggtcagaagc aaaaatctta aattcagtct
     1021 ggccacgttc ggccaggtat gccttcgtgg ttgaatctgc cgcgataatg ccggttttcc
     1081 ctccggcttc aattgccatg ttgcacagcg tcatccggcc ttccatcgaa agaccatcaa
     1141 tcgtggagcc ggtaaactcc atggcggcgt accgcgcacc atccacaccg attttggaga
     1201 tgatgtaaag catcaaatcc ttggcataca ctccctcagg cagttcccct tctaactcaa
     1261 accgaagcgt ttccggcacc ttaaaccaca actcacctgt ggccagcaca gcggccagat
     1321 cggtgctgcc aattccagtg gcaaaggccc caaacgcccc gagcgttgtc gtgtgggaat
     1381 cagcacacac ggcggtcatt ccaggcagga tatggccttt ttcaggaatc agcgcgtgac
     1441 agatgccgtg gttgccaagt tcataatagc gctcgattcc ctgatcaatt gcccattcac
     1501 gcagttcttt ggtgagttgc gccgtttgaa tatccttgtt cggcacaaaa tgatccggcg
     1561 tgaccacaat tcgtttggga tcaaacactt ttgtgatccc aagcttggcc agcatcttga
     1621 tggccggcgg cgtggtaact tcgtgagcga ccaccagatc aatctcagcg ttgacgattt
     1681 cgccgggatg gacggtttca cgtccacagt gggccgcgag gagcttttca gtgatggttt
     1741 gcggttgggc aggcatgggg gattagggtt ccgggttccg ggttccggaa atcattgaaa
     1801 aaacagtgga tttagggaaa caggcataaa caccaaaaaa gagttttagg cccgcagggt
     1861 cgttgtgtaa tagccgtggt gcgaagccca cgggaacaaa aagaatt
//
LOCUS       DAOQDO010001365         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61360_length_1907_cov_2.978942, whole genome shotgun sequence.
ACCESSION   DAOQDO010001365 DAOQDO010000000
VERSION     DAOQDO010001365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61360_length_1907_cov_2.978942"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..>1907)
                     /locus_tag="PKZ53_18900"
     CDS             complement(155..>1907)
                     /locus_tag="PKZ53_18900"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42564.1"
                     /translation="PYGAQSTSGPLTLDVYEPASSSSGLRPLVIFVHGGGFRTGDKSQ
                     GTAYATDFTNAGFVFASIDYRLTGEAIFPAAPTDVLTSIRYFRANAAQWNIDPARIGL
                     FGTSAGANLVLEAAMLGNEGKYEDTAWAGTSQITQAVVSHFGPCDFVNVPVNDGGELA
                     YLGGPPTADPVLAHDASPISWVDADDPPTLIFHGEQDPVVSIRHSELMFARLQETGVV
                     SEFVRVANAGHGFVPTPRGATVTPGVPEIRARTVEFFRRILTTSEAADTTPPRVSVVA
                     PAQGAVLVSGASTPIQWSSSDNTAVTQQDILFSRTGGNTFDSVIATGIGGTTTRFEWQ
                     VGDSQLTEQAVVAVRARDSAGNQGEGRSGLFRIVPRETADTIPPTVAVGAPSTGQILS
                     TGSVSTITWTSSDNLELAAHEIALSRDGGLTFNQTLASGLPANASNFAWTIASSQETS
                     TGVIVVRARDTAGNIGEGRSGLFSIRSSDTQPPVVSQVFVAGGAKKVKRGDSVMITWN
                     TTDDQTVTQHRIQLSTDGGNSFPTPLVEGLAGTAREFTWIVNSSLSKSKTAVIRVQAT
                     DQANNIGQSQSTTFKIK"
BASE COUNT          454 a          487 c          513 g          453 t
ORIGIN      
        1 caatttgggc cattctattt ggagaactga aaaaatttgg gtaatgacaa ggttctgggt
       61 tccgcgcttc cgggttttca aaaccagtca ccaggtgcca ggttttcttc attcttcatt
      121 ctcaattctt cactgctcat tcttcattct gtcgttactt aattttaaaa gttgtggact
      181 ggctttgacc aatattgttg gcctggtcag ttgcctggac gcgaatcact gccgtttttg
      241 actttgaaag ggatgagttg acaatccagg tgaattcacg cgctgttcca gccagacctt
      301 caacaagagg tgtcgggaat gaatttcctc catcagttga aagctgaatt cggtgttggg
      361 tgacagtttg atcgtcagtg gtgttccagg tgatcataac ggagtcgccg cgtttgacct
      421 ttttggcacc gcctgccacg aaaacctgag agaccacagg tggctgggtg tcacttgatc
      481 ggatggaaaa aagacctgag cgaccttccc caatatttcc agccgtatct ctggctctga
      541 ccacaatcac accagttgag gtttcctgac tcgacgcaat ggtccaggca aaattggagg
      601 cattggctgg taatcccgaa gccagggttt ggttgaacgt taatccgcca tcgcgcgaca
      661 gagcgatttc atgagctgcc agttcaagat tgtcggaact ggtccaggtg atggtactta
      721 ctgaaccagt tgacaggatt tggccagtgg atggagcacc gactgcgacg gtgggtggaa
      781 tggtatcagc cgtttctctg ggcacgattc gaaagagacc tgagcggcct tcgccctgat
      841 ttccggcgct atctctggca cggacggcca ccacggcttg ttcagtcaac tgagaatcac
      901 caacctgcca ttcaaaccgg gttgttgttc caccaatccc ggtggcgatt acggaatcaa
      961 atgtatttcc tccagtgcgt gaaaacagaa tatcctgttg agtgactgcc gtgttatctg
     1021 aactgctcca ctggatgggt gtggaggctc cactcacaag aactgctccc tgggcaggtg
     1081 ccacaactga aacccgaggt ggggtggtat cggctgcctc tgaggtggtc aggatgcggc
     1141 ggaagaattc aaccgttcga gcccggatct cagggacacc tggggtaacc gttgccccac
     1201 ggggagtggg gacaaagcca tgcccggcat tggcaacccg aacaaactct gacacgacac
     1261 ctgtttcctg aagtctggca aacatcaatt ccgaatgccg gatagacacc actggatcct
     1321 gttcgccatg aaaaatcagg gtaggtgggt catcggcatc aacccaggaa atcgggctgg
     1381 catcgtgagc cagtaccggg tctgcggttg gaggaccacc aagataggcc agttcgccgc
     1441 cgtcattgac cggcacattg acaaaatcac aggggccaaa gtgcgagacc acagcttgag
     1501 taatctggga ggtgccagcc caggcggtgt cttcatattt tccttcgttc ccgagcatgg
     1561 cggcttccag gaccagattc gccccagccg aagtcccaaa cagtccaatc cgggctggat
     1621 caatattcca ctgtgccgca ttggcccgaa aatagcggat tgaagtcaat acatcagttg
     1681 gcgcggctgg gaaaattgcc tcaccggtca atcggtaatc aattgaggca aatacaaatc
     1741 cagcattggt aaagtcggtg gcgtatgcgg tgccctgact tttatcacca gtgcgaaacc
     1801 ctccaccatg gacaaaaatc accagtggac gcaatcctga agatgacgaa gcgggttcat
     1861 acacatcaag cgttaatgga ccgctcgttg attgcgctcc gtaggga
//
LOCUS       DAOQDO010001366         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61420_length_1906_cov_2.982172, whole genome shotgun sequence.
ACCESSION   DAOQDO010001366 DAOQDO010000000
VERSION     DAOQDO010001366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61420_length_1906_cov_2.982172"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..658
                     /locus_tag="PKZ53_18905"
     CDS             407..658
                     /locus_tag="PKZ53_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42565.1"
                     /translation="MRLFWIVYIAGVILSSPLFYGYMKKYEFALRHLIERELELDDDP
                     ENDKLNEWIYDNAVVLMTCLLSLSWPVSLPDLFKDSDGL"
     gene            843..1175
                     /locus_tag="PKZ53_18910"
     CDS             843..1175
                     /locus_tag="PKZ53_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNJ42566.1"
                     /translation="MEKEKLELLQGTLDMLILKSLVIGQNHGYGIAQWIRQSSENVLS
                     VEEGSLYPALRRLEQRELIASTWGISDNNRRARFYEITPTGRAYLTEETSRWEVMREA
                     ISRVLQTA"
     gene            1191..1652
                     /locus_tag="PKZ53_18915"
     CDS             1191..1652
                     /locus_tag="PKZ53_18915"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HNJ42567.1"
                     /translation="MNAGKRKYHFFNFASRQQALELEIEEELRFHIEMSTQNKIAMGM
                     SPEAAYLDAVQCFGDFEHIKQSCLEIERERLADSPGRKIVKFCCWLVLAGGVIIRLWG
                     ANWCTVDQILIFQHIGSLLIFIALAGWVFITLQSLKPMHSDPGRGRNSLLM"
BASE COUNT          501 a          426 c          486 g          493 t
ORIGIN      
        1 ccagtgtcgg ggcgtcgagc ttgaggccac acgtgatgca aaacgtccag tgtggttcaa
       61 gcgcacttcc acagttacag caggttcggc gatatccggg cagttcagga tgcgaagatt
      121 cagagctggt ttgcgtcata gggtgttccc tccggtgctc attcttaaat gaacagcgtg
      181 agtgtaggca agctggccac cagcatttgg tggaatctgt gtcacttcac caattgacac
      241 agattccatt ctggcctgat tcccagccag acgcaaagat ttctttcaga ggcaaaacct
      301 acttattttc cgcatatttg gagggaattg ctgtgcaact ggcacaaaag ttggtaagct
      361 acctggactc gtttacattc agccttccac gcggagtacc aaagccatgc ggctcttttg
      421 gatcgtctac attgccggtg tcattctttc ttctccatta ttctatggat acatgaagaa
      481 gtatgagttt gccctgcgcc atctcatcga gcgagaactc gaactcgatg acgatccaga
      541 aaacgacaaa ttgaatgagt ggatttatga caacgcggta gttctgatga cctgtttgct
      601 cagcctgagc tggccagtct cgctgccaga tttgtttaaa gacagcgatg ggttgtagct
      661 gagaaaagtt tagaaccccg ccttgaggcg ggtttgctat ctgtagattg aatgtcaggc
      721 ttgatgttgt cccggatgag tttgtcctgt aacccacaac ttgctgatga gtaaattttc
      781 cccttgacaa tctagggaag gactcgttat tttttcccta gataatctag ggagaatgtt
      841 caatggagaa ggaaaaactg gaattgcttc aggggacgtt agacatgctg attctgaagt
      901 cacttgtgat tggccaaaac cacgggtacg gcattgccca gtggatccga caaagctcag
      961 aaaatgtgtt aagcgttgaa gaaggctcgc tgtatccagc actccgccgc ctggagcagc
     1021 gggagctgat tgcctcgacc tgggggattt ctgacaataa tcgccgggca cgattttatg
     1081 aaattacacc gactggccga gcctacctta cggaagaaac ctcgcgatgg gaagtgatgc
     1141 gcgaggcaat ctcacgagtg ctccaaaccg cataggaggc agagtgtctt atgaatgccg
     1201 gaaagcgaaa atatcacttc ttcaactttg ccagccgcca acaagccctc gaattggaaa
     1261 tcgaagagga attgcggttt cacatcgaaa tgagtaccca aaacaagatt gcgatgggga
     1321 tgtcgcccga agctgcctac ctggacgctg tgcaatgttt tggagatttc gaacacatta
     1381 aacagagttg tcttgaaatc gaacgtgaaa gacttgctga ttcaccgggc cggaaaattg
     1441 tcaaattctg ttgttggctg gtgctggcag gcggcgtcat tatccgcctg tggggagcga
     1501 actggtgtac ggttgaccag attttgatat tccaacatat aggctcactg ttgattttta
     1561 tcgctctggc aggttgggtc ttcattacct tacaaagttt gaaaccaatg cattcggatc
     1621 caggtcgagg tcggaacagc ttgttgatgt gagcggacta aaccctgggt ttgcaatgaa
     1681 actcgcctat taaaaacagt caaattggtc aatcaaacaa acctggcaga cgattgacta
     1741 caaacagacg actgattacc aactggtagt atttacaatt ttaaaacaat atcacggcta
     1801 cgtatgccct ttgggcaggt gtgtgtccag ggggaaccac aaatatgagt actgtaccaa
     1861 tggttacaat tgcagggctt gataaaacat atcctggtgg aaccca
//
LOCUS       DAOQDO010001367         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61502_length_1905_cov_1.888649, whole genome shotgun sequence.
ACCESSION   DAOQDO010001367 DAOQDO010000000
VERSION     DAOQDO010001367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61502_length_1905_cov_1.888649"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKZ53_18920"
     CDS             <1..614
                     /locus_tag="PKZ53_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42568.1"
                     /translation="TASLEVRLEGENKDVTYAMKPFTKVSTVWSPSVLGQRKGRIVHK
                     GTMTGPNLTLVGDHILWPTDENLAIQEVQTLVSGKGATLPLSISYLVESFDVDGATPA
                     ENQSRSWTTIQPFSDGGREVSTITFVGYMLYDRYGVDKIADSTTGTFNLTVTNQSNEL
                     ARYRIWLSFSVYRERFDKNGKKTGSSGPSLIHEEVLWFEFRVQ"
     gene            complement(667..1194)
                     /locus_tag="PKZ53_18925"
     CDS             complement(667..1194)
                     /locus_tag="PKZ53_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNJ42569.1"
                     /translation="MTDLRYPVGKFPFERNDYSDADRAAAIEDIASLPQKLRDAVAGL
                     NDEQLDTPYRPDGWTIRQVIHHVADSHVNSYIRFKLALTENEPTIKPYAEALWAEFPD
                     GKSFPVEISLQLLDALHARLVNLLKTRSPEDFDRTLLHPEMGPLNLHKMTALYGWHSR
                     HHLAHITSLKERMGW"
     gene            complement(1467..>1905)
                     /locus_tag="PKZ53_18930"
     CDS             complement(1467..>1905)
                     /locus_tag="PKZ53_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42570.1"
                     /translation="IDLKLKMIKVFSVPRVHTWRFVPKKYFNQEKELTQPIEQLPFIE
                     LRAFSESEDIQRLQSEATQAVEIKIADRLVYWLPEHYDEYNRLSNRYLVMLEDHGVLE
                     FICPVDFKSFTQPGGDTTEKNQGEMVREIKYFIETIKLDPEKK"
BASE COUNT          475 a          452 c          487 g          491 t
ORIGIN      
        1 gaacggcgtc gctggaggtc aggctggaag gtgagaataa ggatgtgacc tatgccatga
       61 aaccctttac caaggtcagt acggtatgga gcccaagcgt tctgggtcag cgcaaagggc
      121 ggattgtgca caagggaacg atgactggtc ccaacctcac cctcgtcggt gaccatattt
      181 tgtggccaac ggatgaaaac ctggccattc aggaagttca aactctggtt tcaggtaaag
      241 gtgccacgct cccactgtct atcagttatc tggtggagtc ttttgacgtt gatggagcga
      301 ctccagccga aaaccagagc cggtcgtgga caaccatcca gccattttca gatggtggcc
      361 gcgaggtctc aaccattacg tttgtcggct atatgctcta tgaccgatat ggggttgata
      421 aaattgctga ttccaccacg ggaaccttca atttgaccgt caccaatcaa tccaatgaac
      481 tggcgcgcta ccgaatctgg cttagttttt cagtgtaccg ggagcgcttt gataagaatg
      541 gaaagaaaac cggcagtagc ggcccgtcac tgattcacga agaagtgctg tggtttgaat
      601 ttcgagttca gtgatggatc tttcgttttc gcccgctcca cagcgggcga aaaccaattt
      661 tgcaccctac cagcccattc gctctttgag cgaagtgata tgtgccagat ggtggcgact
      721 gtgccagcca tacaacgccg tcattttatg aagatttaac gggcccattt cagggtgcag
      781 gagggttcgg tcaaagtctt cgggagatcg ggttttgagc aggttgacca gtcgcgcatg
      841 cagtgcgtcg agcagttgga gtgaaatttc caccgggaag cttttcccat ctggaaactc
      901 agcccagagt gcttcggcat agggcttgat cgttggctca ttttcggtga gtgccagttt
      961 gaatcggata tagctgttga cgtgactatc agccacgtga tggatcacct gccggattgt
     1021 ccagccgtca gggcgatagg gcgtatcaag ttgctcatca tttaaacccg ctacggcgtc
     1081 gcggagcttt tgcggcaggc tggcgatgtc ttcaatcgct gccgcccggt cagcgtcaga
     1141 gtaatcgttg cgttcaaacg ggaattttcc caccggatag cgaaggtcgg tcatagggtg
     1201 tctccaaaag tgtatggtca atcattgatt gaagtgtagc cttacaaaga aagagttccg
     1261 ttcagagtaa cgcttcaggc gtgaaatgtg acaaatgggg tgagggatac agacatctcg
     1321 tctaaagacg atactctgaa ccttattcat cattggtttt gagcggcctg gcataaaacg
     1381 gcgcaccata atccagtccc aaccaccaag gcaaccagtg gaagcgggag aagacagcct
     1441 tccatctcaa acctgtgtga tatccatcat ttcttttctg gatccagttt gatggtttca
     1501 ataaaatact tgatttcacg gaccatctca ccctggtttt tctcagtcgt gtcgccacct
     1561 ggttgagtga aggatttgaa atcaaccggg caaataaact caagcacacc atgatcttca
     1621 agcatcacca gataccggtt tgatagccgg ttatactcgt catagtgttc cgggagccag
     1681 tacacaagcc tgtcagcaat tttgatttca accgcctgtg tcgcttcact ctgcaatcgc
     1741 tgaatatctt cagattcaga gaaagctctc aactcgataa atggaagctg ctcaatcggt
     1801 tgagtgagtt ctttttcctg attgaaatat tttttgggga caaatctcca ggtatgaacc
     1861 cggggaactg aaaaaacctt gatcattttc aacttcaaat caata
//
LOCUS       DAOQDO010001368         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61554_length_1904_cov_1.861547, whole genome shotgun sequence.
ACCESSION   DAOQDO010001368 DAOQDO010000000
VERSION     DAOQDO010001368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61554_length_1904_cov_1.861547"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKZ53_18935"
     CDS             <1..442
                     /locus_tag="PKZ53_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNJ42571.1"
                     /translation="LALFVPLIISSGGNSGSQAATLIIRALALNDARLDDWWIVMQRE
                     LISGLLLGGILGVIGFLRIAVWSLFSPIYGPHWALVGVTVGISLLGVVLLGTLCGSML
                     PFILKRIGADPATSSAPFVATIVDVAGLVMYFTVALLILRGKLL"
     gene            complement(572..967)
                     /locus_tag="PKZ53_18940"
     CDS             complement(572..967)
                     /locus_tag="PKZ53_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42572.1"
                     /translation="MTYDPVKIIGYMAYIPDGTDFQIEGSGVIARSKDLMAAYLAEYQ
                     PEQNVLFQITKVTFFEIPAMSIIKKCAFMIDQPAYERFRKEALKDDIIFPSWHDVQTS
                     EASGKWVKLQFWGNSYELSTKTKFQVIAV"
     gene            complement(1062..1541)
                     /locus_tag="PKZ53_18945"
     CDS             complement(1062..1541)
                     /locus_tag="PKZ53_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42573.1"
                     /translation="MYTVFLGLFLLNPGTINHLPTTNTTADKLPLIGKQNEGDTRFTL
                     GNSPDTAARIQLNKLYQGVLFADGDEGDFFRFSACTGSPLALEVTHGKSTGSPIKFAL
                     RRADQGLFGNRAEVFAYVNPGEAKTISLAEAPACDLIFSVTNDGQDHSYSFKVIGEA"
BASE COUNT          455 a          448 c          493 g          508 t
ORIGIN      
        1 gctggcgctg tttgttcccc tgatcatttc cagcggaggt aactctgggt cccaggctgc
       61 aacgctgatt attcgagccc tggcgcttaa cgatgcccgg cttgatgact ggtggattgt
      121 aatgcagcgt gagttgattt cggggctgtt gctgggtgga attttagggg tgattggttt
      181 tttgcgaatt gcggtatgga gtcttttttc cccgatctac ggtcctcatt gggccctggt
      241 tggcgtcacc gtggggattt cactgcttgg cgtggtgttg ctgggcacgc tctgcgggtc
      301 aatgcttccc ttcattctca agcgcattgg ggctgacccg gccacctcat ccgccccgtt
      361 tgtggcgact atcgtggatg tggcgggcct ggtgatgtac ttcactgttg ccttactgat
      421 tttgcgagga aaattgttgt agccaccgcc tggtaatgag tgccgtttct atatatttta
      481 tattcgggtc tttgtatgag gaaaaagcta aaatattgag aatattatat ttttttaccc
      541 ctttgcctga taccttcagc ccaaactggc attagaccgc gattacctgg aatttggttt
      601 ttgtagaaag ctcgtatgaa ttcccccaaa attgcagctt gacccatttc ccactggcct
      661 cagacgtctg gacgtcatgc caggaaggaa aaataatatc atccttgagc gcttcctttc
      721 gaaagcgttc gtatgccggc tggtcaatca taaaggcaca ctttttgatg atgctcatgg
      781 ccgggatttc aaaaaaagtg actttcgtta tctgaaaaag gacattctgc tcaggctggt
      841 actcagccag atatgctgcc ataaggtctt tcgaccgggc gataacgccg gaaccttcaa
      901 tttgaaagtc ggttccatcg ggaatatacg ccatgtagcc aattattttg actggatcgt
      961 aggtcatagc aggcgcgacc cattgctttg aaaagtggcg actggcgtct ggaaccgatt
     1021 gcaaccaaag gttcagttcc agcaacacca gtcgcttgac ttcaggcttc tccgatgact
     1081 ttaaacgaat aactgtggtc ctgcccgtca ttggtgaccg aaaaaatcag gtcacaggct
     1141 ggggcttcag ccagcgaaat tgtttttgct tcacctggat taacgtacgc aaagacttcg
     1201 gcccggttgc caaacaaccc ttgatcagct cgacgcagtg cgaacttgat gggcgaacca
     1261 gtggacttcc catgggttac ttccaacgcc agcggtgacc cggtgcaggc cgaaaatcga
     1321 aaaaagtcac cttcatcacc atcagcaaac aagacccctt gatagagttt attcaactga
     1381 atgcgagctg ctgtatcagg cgagttcccc agggtaaacc gggtgtctcc ctcattttgt
     1441 tttccgatca gagggagctt gtcggctgtg gtgttggtgg ttggtaagtg gttaatggtt
     1501 ccaggattta acaaaaacaa tccaagaaaa acagtgtaca taaaagaaac tcctgtgcga
     1561 tatgcacgca cacgttcgtt gagcggcaat ttgtcctgca cggtgccttc cgtcaccgac
     1621 tggcacaaac agcctgggga taaacaaata ccgtcccgtc tgatgccaga agtgccacca
     1681 cgggaaccca ttttgattgt tatcagggaa aactcgattg ccgaaaggcg agggggaagg
     1741 tcgagttacg ccacattggt cttgcggctg tgacgcaaca cgtcattaaa cgctatgaag
     1801 tccaaactgg caatagtttt tcgcaaaaaa tttacaggaa agaaacatcg tgttgcagtg
     1861 ccagaaaatg aatggggtgc cgggagaaga tccatgacga ggat
//
LOCUS       DAOQDO010001369         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61570_length_1903_cov_3.320346, whole genome shotgun sequence.
ACCESSION   DAOQDO010001369 DAOQDO010000000
VERSION     DAOQDO010001369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61570_length_1903_cov_3.320346"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKZ53_18950"
     CDS             complement(<1..849)
                     /locus_tag="PKZ53_18950"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ42574.1"
                     /translation="MSETEQQTRRLDQLSVDLDSEGHFATLIMFKEKKVFSHVTLEEE
                     TTLIGRNPDCTITLGADTEASRHHAQVRLSTNTNGLHQYWLSDLGSTNGTHLNGKRLT
                     PHQETLLRDGDKFIIGQHLFKFALLDQSNEKFLTGNLTQVSLFDLIQIIENNRLTCIC
                     IVRGQNEIGKLYFNLGQIVGCEISETQGLEAFRKLVEIQEGFFEVEKHDQEFPVTLEA
                     TSNMNLVLDTLRELDEEHAELLNQETDEQAIPNEMFDTASTGLPENEAMMETHLSESV
                     PVLGIDP"
     gene            complement(1024..1542)
                     /locus_tag="PKZ53_18955"
     CDS             complement(1024..1542)
                     /locus_tag="PKZ53_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42575.1"
                     /translation="MSLLELEAEPKKAEPSQFRKVLIVVGVVILILVGVGVLWSEYRT
                     KQDRVQRSANLVRAGNPEFDAYVKGQKVVVTEMETSESSSMIEFVYELTCQIENRGDR
                     TIEAIELRGFIYDWEDKPSREIYRYPLPSKELPKLGPNQKLKVRMSIDGVKDNSYVKN
                     GLIEIRGLRFAR"
     gene            complement(1562..>1903)
                     /locus_tag="PKZ53_18960"
     CDS             complement(1562..>1903)
                     /locus_tag="PKZ53_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42576.1"
                     /translation="RLIPTNPTPTGGSEAHTYDGEGYIQRASAYFEKSGFYEREIARK
                     SEQFGNIVQVFSTYESLHSPQDTKPFARGINSIQLLKDGNRWWVMTVFWDTERPDNPI
                     PEAYLPKSKTR"
BASE COUNT          443 a          456 c          461 g          543 t
ORIGIN      
        1 ggggtcaatt ccaagcactg gtaccgattc cgagaggtgt gtttccatca tggcttcatt
       61 ttccggcaag cccgtactgg cggtatcaaa catttcattt ggaattgctt gttcatcggt
      121 ttcctggttg agaagttcag catgttcttc gtccagttca cggagcgtat caagcaccag
      181 gttcatattg ctggtggctt caagcgtgac tgggaattcc tggtcgtgtt tttcaacctc
      241 aaaaaaacct tcctggattt caaccagttt tcggaacgct tctaacccct gggtttcact
      301 tatttcacaa ccgacaatct ggcccaaatt aaaatacagt tttccaatct cattttgccc
      361 acgaacaata cagatacagg tcaggcgatt gttttcaatg atttgaatca gatcaaaaag
      421 actgacctga gtcaggttcc cggtcaggaa cttttcattt gattgatcga gcaacgcaaa
      481 tttgaacagg tgttgaccaa tgatgaactt gtctccgtcg cggagcaagg tttcttgatg
      541 gggtgtgagt cgtttcccgt ttaagtgggt gccgttggtg cttcccaggt cgctgagcca
      601 gtattgatgt aatccattgg tattggtgct gagccgcacc tgggcatgat ggcgtgaggc
      661 ttcagtatcc gccccgagtg tgatggtgca gtctggattg cggccaatga gcgtggtttc
      721 ttcttccagc gtgacgtgcg aaaagacctt tttttcctta aacataatta aggtggcaaa
      781 atgcccttcg ctgtccagat cgacagaaag ttggtcgagt cgccgggtct gttgctcagt
      841 ttcgctcata aacggtactc ctcaagtgaa gtgatgtggg caggacagcg aaagtgaatc
      901 cgtgttgaac ttcaatgtga ttgggttgag gctgagagtg caagtgtggt caccagatgg
      961 ggtttgtgaa aactactaaa ttgcaccgcc atttttcaat cgaaagcgta cgtttccaga
     1021 ttctcaacgg gcaaaacgca ggccccgaat ctcaatcagt ccatttttga cataactatt
     1081 gtccttgacg ccgtcaattg acattcggac cttgagcttc tggttggggc caagtttggg
     1141 aagttccttg ctgggcaacg gatagcgata aatttcgcgc gaaggtttat cttcccaatc
     1201 atagataaat cctcgcaatt cgatggcttc aatcgtccga tccccccggt tttcaatctg
     1261 acaggtgagt tcatatacaa actcaatcat gctgctggat tctgaggttt ccatctcggt
     1321 cacaacaact ttttgccctt tgacataggc atcgaattcc ggattcccgg cacggaccag
     1381 attggctgaa cgctgaaccc ggtcttgttt ggttcggtac tcgctccaca agaccccaac
     1441 gcccaccaga atcaggatca ccacacccac cacgattaga acttttcgaa attgcgaagg
     1501 ttcagccttt ttcggttcag cttcgagttc aagcagcgac acgccaatca ttcctccaac
     1561 gttacctggt ttttgatttc gggagatagg cttctggaat cgggttgtca ggtcgttcgg
     1621 tgtcccaaaa aactgtcatc acccaccatc ggttcccatc tttgagtaac tgaatgctgt
     1681 taatgccacg agcaaagggt ttagtgtcct gcggtgaatg cagggattca taagtgctaa
     1741 acacctgcac gatatttccg aactgctcgc ttttccgggc aatttcgcgc tcgtaaaaac
     1801 cgcttttttc aaagtaggcc gaggcgcgtt gaatatagcc ttcgccatca taggtatggg
     1861 cttcgctgcc gccagtcgga gttggattgg ttggaatcaa tct
//
LOCUS       DAOQDO010001370         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61597_length_1903_cov_2.414502, whole genome shotgun sequence.
ACCESSION   DAOQDO010001370 DAOQDO010000000
VERSION     DAOQDO010001370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61597_length_1903_cov_2.414502"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1565)
                     /locus_tag="PKZ53_18965"
     CDS             complement(54..1565)
                     /locus_tag="PKZ53_18965"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNJ42577.1"
                     /translation="MSLFKRHFFLVCLVAGLSGLLVWSELASGAARPGGGHSYGGSRS
                     SGGGSGGGGGGEGLFFLLELIFRLIFYYPKIGIPLLLIVIVWFLYSSRHSRVPSYSSR
                     MPQQWEPLPSPATISQRSDALRVHDPNFSEILFMDFVAALYGRVHEARGRRELEPYRS
                     FVSLDALNQLLYQTPADVVGVTGIVLGSATIIDFSPVSEPVITLTVEFETNYTELNRN
                     GEGQTTWYARERWAFQRKRDLLSRPPEKITVDRCPKCNGPLEKRQDGSCAFCGVMILG
                     GDFDWFVIRIELLSREDRGPALTQDVPEQGTDLPTVFQPNVDLVFKQFTVLNPTFQWG
                     VFEKRVQHIFHQIYQAWTSLDWEQARPFETDNVFQMHRYWIREYERQGLRNVLKDIQI
                     ARIDLAKVKADAFFDAITVRIFASCIDYTADLAGTILSGKPNKPRIFSEYWTFIRRRG
                     VAAVSKGNSSCPNCGAPLHINMAGVCEYCGSKITSGDFDWVLSRIEQDDAYRG"
     gene            complement(1562..>1903)
                     /locus_tag="PKZ53_18970"
     CDS             complement(1562..>1903)
                     /locus_tag="PKZ53_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42578.1"
                     /translation="TSSDRRSRQVQAAARAAFVEFEILHTRSRTGVLIYWSVVEQQVE
                     IIADLGVMAAIPREAWKQALAEFQMNKPTRQRMSDFPEDLLTFGQFLSRYLPATADNP
                     DELPNRPRILS"
BASE COUNT          484 a          536 c          491 g          392 t
ORIGIN      
        1 cggaacccgg aacccggaac ccggaacccg gaacccggaa cccggaaccc ggattagccc
       61 cgataggcgt catcctgttc gatgcggctc agcacccagt caaaatcgcc gctggtaatt
      121 ttgctgccac aatactcaca gacccctgcc atgttgatat ggagcggcgc gccgcaattg
      181 gggcagcttg aattcccctt cgataccgcc gccacacccc ggcgacgaat aaaagtccag
      241 tattcagaaa agatacgggg tttatttggc ttgccggata ggatggtacc ggctaaatct
      301 gccgtgtaat caatgcaact ggcgaaaatt cggacggtga tggcgtcaaa aaaggcatca
      361 gccttcactt tggccaggtc aatccgggca atttgaatat ctttgagcac gttgcgcagc
      421 ccctggcgct catattcgcg aatccaatag cggtgcatct ggaacacgtt gtctgtttca
      481 aacggacgtg cctgctccca atcgagcgag gtccaggcct gataaatttg atgaaaaatg
      541 tgctggacgc gcttttcaaa gactccccac tgaaaggttg gattcaacac ggtgaactgc
      601 ttaaacacca gatcaacatt tggttgaaat acggttggca ggtcagtgcc ctgctcgggt
      661 acatcctgtg tcagagcagg cccgcgatct tcacggctca ggagttcaat tcgaatcaca
      721 aaccagtcaa agtctccgcc cagaatcatc acgccacaga aagcacagct tccatcctga
      781 cgtttctcaa gcggcccatt gcactttgga caccggtcca cggtaatttt ttccggtgga
      841 cgcgacagca gatcccgttt tcgctgaaac gcccagcgtt cacgggcata ccaggtggtc
      901 tggccttcac cattgcgatt gagttcggtg taattggtct caaattcaac agtgagggta
      961 atgaccggct ccgaaaccgg gctaaagtca atgatggtcg ccgaccccag cacgataccg
     1021 gtcaccccaa ccacatccgc cggagtttga tacaaaagct gatttaacgc atccagactg
     1081 acaaatgacc ggtacggctc aagttctcgc cggccacgag cttcgtggac tcgaccatac
     1141 agcgcggcga caaaatccat gaagagaatt tctgagaagt tagggtcatg cacccgaagg
     1201 gcatctgacc gctgggaaat cgttgctggg gacggcagag gttcccattg ttgcggcatc
     1261 cgggatgaat atgatggcac ccggctgtgg cgtgaactat agagaaacca cacaatgaca
     1321 atgagtaaca ggggaattcc aattttgggg tagtaaaaga tcaggcgaaa aatcagctcc
     1381 aacaagaaaa aaagtccttc gccaccgcct cctcctccac tgccgccgcc actgcttcgg
     1441 cttccaccat acgagtgacc acccccagga cgagccgccc ctgaggccag ttcagaccag
     1501 accagcagac ctgacaggcc cgccaccaga cacaccagga aaaaatgtcg tttgaaaagg
     1561 ctcatgacag aatgcgcgga cggtttggaa gttcgtcggg gttatcagcc gtggctggga
     1621 gatagcggct cagaaattgg ccaaaggtca gcaggtcttc tggaaaatca gacattcgtt
     1681 gccgcgtggg cttattcatt tggaattcag ccagagcctg cttccaggct tcacgtggaa
     1741 tggccgccat caccccaaga tctgcaatga tttcgacttg ctgctccacc actgaccagt
     1801 aaattaaaac tccggttcgg ctccgggtat gcaggatttc aaactcgaca aatgcagcac
     1861 gggccgccgc ctggacctgt cgggaacgcc ggtctgatga agt
//
LOCUS       DAOQDO010001371         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61679_length_1902_cov_2.130482, whole genome shotgun sequence.
ACCESSION   DAOQDO010001371 DAOQDO010000000
VERSION     DAOQDO010001371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61679_length_1902_cov_2.130482"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..>1902)
                     /locus_tag="PKZ53_18975"
     CDS             complement(87..>1902)
                     /locus_tag="PKZ53_18975"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42579.1"
                     /translation="PCSRSISMRKYLIVPAFLLAMLLWPAFVSGKEDWRRVRSANFTI
                     VGDASENQMKKLADRLEQFRQSLQIVLPKANLSDAVPTTVYLFQDESSFHPFKPKYEG
                     KIRDNVAGYFLPRSTTNYIVLSNDALRSVARSANSKLSNPFRLIFHEYFHFVVQNNLD
                     FAPIWLNEGLAEYYSTFETTDEGQVIQLGAPITLHIQILREKQLLPLETLFTVDRKSP
                     HYNESNKTSIFYAQSWALVHYLALGNNQKRAAQFNQFLTLVSADRPQLESFRQAFQTD
                     PKSLEDELRDYVRKFSFPVLNVKLPKQIDYTKDLQTSLLSETDSLFLQGELLAQLRRD
                     QEAEEYFKKTLKLAPKHSATLLSLGIIRLRQEKPAEAKKYVEEALAADPRNSQAHYQY
                     ARMLRQEGQKPDAIKALHQAISLQPTMAQAFADLGFLYLQTGEREKALEAFAQAIKLD
                     PQNTDLLRTTSYTLLGHRQGKAAADDAIAYLKRKRWRDEHALYMTLVIHFGSREAHNQ
                     AIAVKILSEAEANANTANWPFPVIRYLQRRLSETELLAQATDTDKLTEAHAYVGLDLL
                     LNGKPEEAQPHFKWVVDHGNRNFVEYTLTLAEIERLQK"
BASE COUNT          431 a          430 c          509 g          532 t
ORIGIN      
        1 aaccatatct cattcggata tttttcaata acttcggaat cctaatctag atgcagtaca
       61 tagttagcgt gtcttgagac cttgggttat ttttggagtc gctctatctc agccagcgtg
      121 agtgtgtatt caacaaaatt ccggttccca tggtccacca cccatttgaa gtgaggttgt
      181 gcttcttccg gtttgccgtt gagcaataaa tcaagcccga cgtaggcatg cgcttcggtc
      241 aatttgtcgg tatctgtcgc ctgagccaga agttcggttt cactgagacg gcgttgcaga
      301 taccgaataa ccggaaatgg ccagttggcg gtgttggcat tggcttcggc ttcgctgagt
      361 attttgacgg caatggcctg attgtgtgct tcccgagacc cgaaatggat aaccagggtc
      421 atgtagaggg catgttcgtc acgccagcgc ttgcgtttaa ggtaggcaat ggcatcatct
      481 gccgccgctt tcccctggcg gtgaccaagc aacgtgtaac tggtggttcg aagcagatca
      541 gtgttctgcg ggtcaagctt gatggcctgg gcaaaggctt ccagggcttt ttcacgctca
      601 ccagtttgga gatagagaaa acctaaatcg gcaaatgcct gggccatggt tggctggagc
      661 gagatcgcct ggtgcaatgc cttgatagca tctggttttt gaccttcttg cctgagcatc
      721 cgggcatatt gatagtgtgc ctgactgttt ctgggatctg ccgccagagc ttcttcaaca
      781 tactttttgg cttcggcggg cttttcttgt cgtaaccgga tgattccaag cgagagcaac
      841 gtggctgaat gtttcggcgc aagttttaag gttttcttga aatattcttc ggcttcctga
      901 tcgcgtctca actgtgccag tagctctccc tggaggaaaa gactgtcagt ttctgacagc
      961 aagctggttt gcaaatcctt tgtgtaatca atctgtttgg gaagtttgac attgagcacc
     1021 gggaaagaga actttcgcac atagtcacgg agttcatctt ccaggctctt ggggtctgtt
     1081 tgaaacgcct gacgaaagct ttcgagctgg ggccggtcgg cactgaccag ggtcaggaat
     1141 tgattgaact gcgctgcacg tttctggttg tttcccaatg ccaggtagtg aaccagcgcc
     1201 caggattggg cataaaaaat gctggttttg ttgctttcat tgtaatgggg tgatttccgg
     1261 tcaacggtaa acagcgtttc cagcggcaat aactgctttt ccctcagtat ctggatgtga
     1321 agggtgatag gtgcccccag ttggatgacc tgcccctcgt ctgtggtttc aaaggtgctg
     1381 tagtattcag cgagtccttc atttaaccag attggcgcga aatcaagatt gttttggacc
     1441 acaaagtgaa aatactcgtg gaagatcaac cggaatgggt tgctcaattt tgaattggca
     1501 gaacgggcga ccgaccggag ggcatcgttt gaaagcacga tgtaatttgt cgttgatctt
     1561 gggaggaaat agcctgcgac attgtcgcgg atctttcctt catattttgg tttgaacgga
     1621 tgaaaagagc tttcatcttg aaacagatag accgttgtcg gcaccgcatc gctcagattg
     1681 gctttgggga gcacaatctg gagcgactga cgaaattgct ccaggcggtc agccagtttc
     1741 ttcatttggt tttcagacgc atcgccgaca attgtgaaat tggctgagcg aacccgcctc
     1801 caatcctctt ttcctgagac aaaagcaggc cagagcaaca ttgccagcag aaaagcaggc
     1861 acaatcaaat attttctcat tgagatactc ctggaacagg gt
//
LOCUS       DAOQDO010001372         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61739_length_1901_cov_2.596966, whole genome shotgun sequence.
ACCESSION   DAOQDO010001372 DAOQDO010000000
VERSION     DAOQDO010001372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61739_length_1901_cov_2.596966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1002
                     /gene="rsmI"
                     /locus_tag="PKZ53_18980"
     CDS             157..1002
                     /gene="rsmI"
                     /locus_tag="PKZ53_18980"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNJ42580.1"
                     /translation="MSGTLYLVPTPIGNLEDITLRALRILREVDLIACEDTRHTRKLL
                     SHFDIHTPTLSYHEHNERDRVTRLLGDLQSGKSVAIVSDAGMPGISDPGVVIVQAAVE
                     AGCPVTALPGACAFPTALAASGLNTEQFLFAGFLPVKQGARRQALEGVRDLLVTLVFY
                     EAPHRISAFLEDALLVLGNRPAVVARELSKQFEEYIRLPLRAQIEHFNQHEPRGEMVI
                     LIAGKSQDVQTEPLSPRLTLREDMERLIAGGISPNAAMKQLSQKYGVAKRELYRQWQE
                     EKNEE"
     gene            1158..>1901
                     /locus_tag="PKZ53_18985"
     CDS             1158..>1901
                     /locus_tag="PKZ53_18985"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNJ42581.1"
                     /translation="MCGICGVFEFGRAQGEVSEQLLTAMSDVIVHRGPDSGGTYVSPD
                     RRLGLGFRRLAIVDLSAAGNQPMTNEDGSLWLVFNGEIYNHRVWRQQMLEKGHVYRSE
                     ADTETILHLYEDRGREFVHCLHGMFGIALWDSVKEELTLVRDRIGVKPVYYTVADGRL
                     IFGSEIKAILEHPAVVRDVDETALYHYLTFVTTPAPQTLFKGIRKLPPGHMLICDRKG
                     NIRIERYWDAIVETPQQELTEAEIIPRLRD"
BASE COUNT          441 a          469 c          495 g          496 t
ORIGIN      
        1 attgaagttc agatcgccgg acggaaaaat tttaaagtgc tcacccggtc aggctacctt
       61 gctacacctc agtaagtacc tccaaccgtc aattcatggt gaattcatag ggctgagggc
      121 tttcaggcac tcagcctttg ttcttcaagc ttctttatgt ctggcacgtt atatttagtt
      181 cccaccccga ttggtaacct cgaagacatc accctgcggg ccttgcgaat cctgcgtgaa
      241 gttgacctga ttgcctgcga agatacccgc catacccgca aactcctttc tcactttgat
      301 attcacacac caactttgag ttatcacgag cacaatgaac gtgatcgggt gacccgattg
      361 ctcggtgacc tccagagcgg aaaatcagtc gcgattgtga gtgatgctgg aatgcccgga
      421 atttcagacc ctggcgttgt gattgtccag gccgctgtcg aagctggctg cccggtcacc
      481 gccttgcctg gtgcctgtgc cttcccgacg gcgctggcgg cttcgggact gaacaccgaa
      541 cagttcttat ttgctggttt ccttcctgtt aaacaaggtg cccgtcgtca ggcactggaa
      601 ggtgtgcgcg atctgttggt aactttggtc ttttatgaag caccacaccg catttcagcc
      661 tttcttgaag acgccttgct ggtgctcggg aatcgcccgg cggtggttgc ccgcgagctt
      721 tcaaaacagt ttgaagagta tattcgcctg ccgttacgtg cccagattga acattttaac
      781 cagcatgaac cgcgtggtga gatggtaatt ctgattgctg gaaagagtca ggacgttcaa
      841 actgagccgc tttcaccgcg cctgactttg cgagaagata tggaacggtt gattgctggt
      901 gggatcagcc ccaatgcggc catgaaacaa ctctcacaga aatatggtgt tgccaaacgc
      961 gaactatatc gccagtggca ggaagagaag aatgaagaat gaagaaagtg gctagtggtt
     1021 agaaatcaat agcttcgaag cccgaagcgc cagtcaccag atagcagtca tcagataagt
     1081 ccttcattct cagttcttca ttcttcattc tcaattcttc attctcaatt cttcattctc
     1141 aattctttat tcaatttatg tgtggaattt gcggcgtttt tgaatttggt cgggctcagg
     1201 gcgaagtttc tgagcaattg ttaaccgcga tgagcgatgt aattgtgcat cgtggaccag
     1261 atagcggagg cacctatgtc agtccggatc gtcggctggg cctggggttt cgtcggctgg
     1321 cgattgtgga cctgtcagcg gcgggaaatc agcccatgac aaatgaagac ggttcgttgt
     1381 ggctggtctt caacggtgaa atctataacc accgggtctg gcgacagcaa atgctcgaaa
     1441 aggggcacgt gtatcgttct gaagcggata ctgaaaccat tctgcacctc tatgaagacc
     1501 gtggccgcga gtttgtgcat tgcctgcacg gcatgtttgg aattgcgctc tgggatagcg
     1561 ttaaagagga actcacgctg gttcgggatc gaatcggggt caagccggtt tattacacgg
     1621 ttgctgatgg tcggttgatc tttggttcgg aaatcaaagc cattcttgaa catccggcgg
     1681 ttgtgcgtga tgtggatgaa accgcgctct atcactacct gacatttgtc accacgccag
     1741 caccgcaaac cctgttcaaa ggtattcgta agctcccgcc aggacatatg ctcatctgtg
     1801 atcgaaaggg aaacatccgg attgagcgtt actgggatgc aattgttgaa acgccacaac
     1861 aagaacttac cgaagccgaa atcattcctc gcctgcgtga c
//
LOCUS       DAOQDO010001373         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61810_length_1900_cov_3.297019, whole genome shotgun sequence.
ACCESSION   DAOQDO010001373 DAOQDO010000000
VERSION     DAOQDO010001373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61810_length_1900_cov_3.297019"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="PKZ53_18990"
     CDS             <1..150
                     /locus_tag="PKZ53_18990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42582.1"
                     /translation="DPLYTTKEKGRGTGLGLVVVHQIMRDHHGHIDVESTPGQGTCFR
                     LSFPI"
     gene            235..642
                     /locus_tag="PKZ53_18995"
     CDS             235..642
                     /locus_tag="PKZ53_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42583.1"
                     /translation="MLIAQAGWAAIAKHHACIARPAGTTYPRCSAYINSRTRYKRCLS
                     VTLGNQGIYIEPVIFFRVAHPPLLIPFSCVTGLAPGEPAFGNTIRVDISAAGHDIKLY
                     VPVECRPFLDLHKYPWKPVFATQNHIGVEGVLN"
     gene            794..>1900
                     /locus_tag="PKZ53_19000"
     CDS             794..>1900
                     /locus_tag="PKZ53_19000"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ42584.1"
                     /translation="MKQILVIDDDPASCELLKEILADQGWNIETAQLPDQAVKLFQKK
                     QFDLVITDFNLEATQTGLDLVKQFRDRCPVVLVTAFGTLETAVDASREGAWDFISKPF
                     KVDEVVATASRALEHQSQKSQAGSLPDSESLLARYAGSGMVGRAPAMIELYKEIARVS
                     SARSTVLIAGESGTGKELVARAIHTHSPRADKPFIAVNCGALTETLLEAELFGHVRGA
                     FTGATNDRKGLWEEAEGGTLFLDEIGETTPALQVKLLRALQEGEIKRVGSSRTTKVDA
                     RIVAATNRDLEKEVKAGNFREDLFYRLSVVTLNVPPLRERRMDIPVLVERFLQKSAVS
                     VGRRLRASESALAIFVAYDWPGNVRELEAAVEYAA"
BASE COUNT          444 a          505 c          502 g          449 t
ORIGIN      
        1 gatccgctct ataccaccaa agaaaagggc cgtggcacgg gccttgggct ggttgtggtt
       61 caccagatca tgcgcgacca ccacggccac attgacgtcg aaagcacccc tggtcaagga
      121 acgtgtttcc ggctttcttt tccaatttga acaagggcaa tcctcatgtg gccttcactt
      181 gcaatttttc tggtcgtttt ggtggtcttt cctctgtttt ggagctcgat tgttgtgctc
      241 atcgctcaag ccggatgggc ggccattgcc aaacaccatg cctgcatcgc ccgccctgcg
      301 ggaaccacct atccacgatg ctcggcctat atcaattccc gcacccgcta taaacgctgc
      361 ctcagcgtta cgcttggcaa tcaaggaatt tatattgagc cagttatctt ttttcgagtg
      421 gcgcatcctc cgttgctgat tcctttttcc tgtgtgaccg gactggcacc aggtgaacca
      481 gcttttggaa acacgatccg ggttgatatt tcagccgccg gccatgacat caaactatat
      541 gttcccgtcg aatgccggcc atttctggat ctgcacaagt acccctggaa accagtcttt
      601 gcaacccaaa accacattgg ggttgaaggc gtcttgaatt aaatgagtga aggaacgaca
      661 gagcgaactt tgaggctggt tatactgctg tcaacaacga aacgatgaac tcctcgtatt
      721 gacaaacctt caagtgattc aatcaaataa actgagtgaa ctacggacta ccagctactg
      781 acgacaaact gatatgaaac aaatacttgt cattgatgat gaccccgcgt cgtgtgaatt
      841 actcaaagag attttggctg atcagggttg gaatatcgaa acggctcaac tccccgacca
      901 ggccgtgaaa ctgttccaga aaaaacaatt tgatctggtc atcaccgatt tcaatctgga
      961 agcgacacaa actggcctgg atctggtcaa acagttccgg gaccggtgcc cggtggtttt
     1021 ggtgacagcc tttggcacgt tggaaacagc ggttgacgcg tcacgcgaag gggcgtggga
     1081 ttttatttcc aagcccttta aagtggatga agtggtggca accgccagcc gggccctcga
     1141 acaccagagt cagaaaagtc aggcaggctc gctcccggat tctgagtcac tactggcgcg
     1201 gtacgcaggc tccggcatgg tcgggcgagc cccagcaatg attgagttgt ataaagaaat
     1261 tgcccgcgtg tcgtcggccc ggtcaacggt tctgattgcc ggcgaatccg gcacgggcaa
     1321 ggaactggtc gcccgtgcca ttcataccca tagccccaga gcagataaac catttattgc
     1381 cgtcaattgt ggtgcactga ccgaaactct gctggaagcc gaactctttg gtcatgtccg
     1441 gggtgccttc acgggtgcca caaatgatcg caagggtctc tgggaagaag ccgaaggcgg
     1501 cacgctgttt ctggatgaaa ttggggaaac cacgcccgcc ttgcaggtca aactcctgcg
     1561 ggcgcttcag gaaggtgaaa tcaaacgagt tgggtcctca cgcacgacca aagtggatgc
     1621 ccgcatcgtg gcggcgacca atcgtgattt ggaaaaagaa gtcaaagccg gtaattttcg
     1681 tgaagacctg ttttaccgcc tgagcgttgt caccctcaat gttccgcccc tgcgtgaacg
     1741 ccgaatggac attccggttt tggtcgagcg gttcctccaa aaatcagcgg tttcggttgg
     1801 tcgccgcctc cgggcatcag agtcagccct ggctattttt gtggcctacg actggcctgg
     1861 aaatgtacgc gaacttgagg cagcggttga atatgcggcc
//
LOCUS       DAOQDO010001374         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61860_length_1899_cov_3.246204, whole genome shotgun sequence.
ACCESSION   DAOQDO010001374 DAOQDO010000000
VERSION     DAOQDO010001374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61860_length_1899_cov_3.246204"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1899
                     /locus_tag="PKZ53_19005"
     CDS             <1..>1899
                     /locus_tag="PKZ53_19005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ42585.1"
                     /translation="IYDEASGLPDATAFALLETTRIENKQIIWAGMRGGGIARLVNGR
                     WKLEPSDFGLETATVRRMIETVAEDGTPEIWAATGSHGLAHYRNGKWTAITTRDGLPT
                     NRLFTVVEAVNSAGERQLWVGTEGGGLACRSKDGSWKVWNQASGLPNNSILSLLVVNG
                     PDGTPTLWAGTEGGGVVRLALTPGNQTFAVFSEQSQPALPNNTIYQVQSDARGRMYLF
                     TNKGVARLSPRTPTLANPAEFDVYTFSIEDGLPNNEFNGGASCTDARGRIWGGSIGGA
                     VVYDPGREVPEAGAKPLKLERFLINGEPVELKATGNAAYFTLERALRYDENNLLVEFS
                     LLSFFREKNTAYQIQLDGFDHAKPQWMTDFKKEYTNLPAGTFTLSVWGRDAGGTISGP
                     LVVSFRVKSAPWLTWWAFLGYGLVAGSGIWFGARWRMRALEQRNLVLESKISERTADL
                     AQAVKETERKNQLLEAAKDQVERKNQELDRKIAELMASQQQADRIFSALAEALPGTVL
                     DGKYRLEEKIGSGGFGTVFRGIHLTLDRPVAVKVFRPRPGNDTPEAVERFRREGVSAT
                     RVNHPNAIQVLDWGISTEGIAFLVMELLTGHSLGDELKRQKRLTLNRMKAILQPVCSA
                     LAEAHSVGI"
BASE COUNT          449 a          482 c          553 g          415 t
ORIGIN      
        1 atttatgacg aagcttccgg tttgccagat gccacggcgt ttgcgctcct ggaaacaacc
       61 cggattgaaa acaaacaaat catctgggcc ggtatgcgcg gtggcggtat tgcccggctg
      121 gtcaatggcc gctggaagct ggaaccctcg gattttggtc tggaaaccgc gaccgttcgg
      181 cggatgatcg aaaccgttgc cgaagatggt acgcctgaaa tttgggcggc gaccggatct
      241 cacggactgg ctcactatcg aaatgggaaa tggaccgcca tcaccacccg tgatgggctg
      301 ccaaccaacc ggttgtttac cgtggtggaa gcggttaatt ccgctggcga acgtcaactc
      361 tgggttggaa ctgaaggcgg tgggctggct tgccggtcca aagatggatc atggaaggtg
      421 tggaaccagg cttccggctt gccaaataac agcattcttt cgctcctggt tgtcaacggc
      481 ccggatggaa caccaacact gtgggcggga acagaaggcg gtggggttgt ccgattggcg
      541 ttgacgcctg gaaatcaaac atttgcagtc ttttcagagc agtcacagcc ggcccttccc
      601 aacaacacga tttaccaggt tcaatccgat gccagaggcc gaatgtatct gtttaccaat
      661 aaaggtgtgg cccggttatc gcctcgaacg ccaacccttg ccaatcccgc cgaatttgat
      721 gtctacacgt tttcaattga ggatggcctt ccaaataatg agtttaacgg tggagcttcg
      781 tgcactgacg ccagaggtcg aatttggggt ggctcgattg gcggggccgt ggtgtatgac
      841 ccaggccgcg aagtccccga agctggggca aaaccattga aactggaacg atttctgatc
      901 aatggcgaac cagttgaact caaagcgact ggaaatgccg cctatttcac attggagcgg
      961 gcactccgct atgatgaaaa caatctgctg gtggaatttt cgctgctgag tttttttcga
     1021 gagaaaaaca cggcgtacca gatccagctc gatggatttg atcacgccaa accccaatgg
     1081 atgactgact ttaaaaagga atacacgaac ctcccggctg gaacattcac gctgagtgtg
     1141 tgggggcggg atgcgggtgg caccatttct ggtccactgg tggtgtcgtt cagggtcaaa
     1201 tcagccccct ggctgacgtg gtgggcattt ctcgggtatg ggctggttgc cgggagtgga
     1261 atctggtttg gcgcgcgctg gcgaatgcgt gctctcgaac aacgtaacct ggtgctcgaa
     1321 tctaaaatct ctgagcgaac cgctgacctg gctcaggcgg tcaaagaaac cgaacgcaaa
     1381 aaccagctcc tcgaagccgc caaagatcag gtcgaacgca aaaaccagga gcttgatcga
     1441 aaaatcgcag aactgatggc ctcgcagcag caagctgacc gaatcttttc agctctggct
     1501 gaagccttgc cgggaaccgt gctcgatggg aaatatcgcc tggaagaaaa aattgggtct
     1561 ggcggatttg gaacggtttt tcgaggtatt cacctgacac ttgaccgtcc agttgccgtc
     1621 aaagtgtttc ggccacgacc tggaaacgac acacccgaag ccgtcgaacg gtttcggcgc
     1681 gaaggtgttt cggccacacg ggtcaatcat ccgaatgcca tccaggtgct tgattggggg
     1741 atttcaaccg aaggaattgc gtttctggtc atggagcttc tgaccgggca ctcactgggc
     1801 gatgaactca agcgccagaa acgattgacg ctcaaccgga tgaaagcgat tctccagccg
     1861 gtatgctcgg cactggcaga agcgcattcg gttggaatc
//
LOCUS       DAOQDO010001375         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61871_length_1899_cov_2.708785, whole genome shotgun sequence.
ACCESSION   DAOQDO010001375 DAOQDO010000000
VERSION     DAOQDO010001375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61871_length_1899_cov_2.708785"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PKZ53_19010"
     CDS             <1..1454
                     /locus_tag="PKZ53_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNJ42586.1"
                     /translation="PEPGTLNSSHPMSQSKEITPNTHDTFPMWKVIVMIVFPLSVWFS
                     PLSLSPEGRKALTIASLMIAAWGTEIFSHTLVGLIGCFLFWVCDVVEFSKAFGGFASS
                     TPWFCYSAVLFGTALSQSGLTRHLAYWVMLQYGSTYSRLIFGFTLLSILLTFMVPSGP
                     ACVVIKAAIALGIMEATGFKPGSNVGRGIFVVLTYGASLFNKMVIAGASSIMARDMIE
                     AQTGVKMFWSYWFLAFLPVSLISLWVCYRLTLWLFPPEKESLVESAVYLREELKKIEG
                     WSKPKIWAAVFATLALSLWITDFWHHISPAKIGIGISLLAAIPFFGLLQSDDLKKVNY
                     LPAFFVGTAASMGEVLVKTGATEALTTILFGWMTPLMTSPLSAVLTTFWTAFVYHLFL
                     GSEISMLSTSMTPLLQFAQSHRLNAVAIGLIWTFAGACKVFVYQSTIVMIGYSYGYFE
                     ARDIVKFGAVIAVVESIIMVLVSLFYWPLIGLG"
BASE COUNT          403 a          427 c          528 g          541 t
ORIGIN      
        1 acccggaacc cggaaccctt aatagttctc atccaatgtc acaatccaaa gaaatcacgc
       61 cgaacaccca cgataccttt ccgatgtgga aggtgatcgt gatgattgtt tttccactga
      121 gtgtctggtt ttcgccgctt tcactgtcac ctgaaggccg aaaagccctg acgattgcca
      181 gtttgatgat tgcggcctgg ggaactgaaa ttttctcgca caccctggtc gggctgattg
      241 ggtgtttttt gttttgggtg tgcgacgtcg tcgaattctc aaaagcgttt ggcgggtttg
      301 cttcgtccac accgtggttt tgttacagcg cggtgctttt tggaacggca ctgtcacaat
      361 ccgggttaac ccggcatctg gcctattggg tgatgctaca gtatgggagc acctattctc
      421 ggttaatttt cgggtttacc ttactttcaa tcctgctcac ctttatggtt ccttccgggc
      481 cagcctgtgt tgtgatcaaa gcagctatcg cgctcggaat catggaggcc actgggttta
      541 agccaggaag caacgtcggg cgcggcatct ttgtcgtgct gacctatggt gccagcttat
      601 ttaacaaaat ggtgattgcc ggggcgtcat ccattatggc gcgggatatg atcgaagccc
      661 aaaccggtgt aaagatgttt tggagctact ggttcctggc ctttctgcca gtctctttga
      721 tttcgctgtg ggtgtgttat cggctgacgc tctggctctt tccacctgaa aaagaaagcc
      781 tggtcgaaag cgcggtttat ttgcgggaag aactcaaaaa aattgaaggc tggtccaaac
      841 caaaaatctg ggcggctgta tttgcaaccc ttgccttgag tctatggata accgatttct
      901 ggcatcatat ttccccagcc aaaattggaa ttggcatcag cctgctcgcc gcgatacctt
      961 tttttgggtt gttacaatcg gatgatttga agaaagtgaa ttacttaccg gcattttttg
     1021 tcggtacagc cgccagtatg ggcgaggttc tggttaaaac tggcgccacg gaagccctga
     1081 cgacgatttt gtttggctgg atgacacccc tgatgaccag cccgttgagt gccgttctga
     1141 cgacgttctg gacggcgttt gtgtatcatt tatttttggg gagcgaaatt tcaatgctca
     1201 gcacgtcaat gaccccgctc ctgcaatttg cccaatctca ccgtctcaat gcggttgcga
     1261 ttggactcat ctggacattt gctggcgcct gcaaagtctt tgtgtatcaa tcaacgattg
     1321 ttatgattgg gtattcctac gggtattttg aagcgcgcga cattgtaaaa tttggcgccg
     1381 tgattgccgt ggttgaatca atcatcatgg tactggtgag cctgttctac tggccgctga
     1441 ttgggctggg gtgagaaaac cagggctgaa aaaccagggg ctaagggctt ggggctgaag
     1501 aagacgggct gaagaaggcg ggctgaaaaa ccaggggtta agggctcata agcgccagga
     1561 taaggatttc aggcccgcag ggtcgttgtg taatagccgt ggtgcgaagc ccacggtcac
     1621 ggccaatgtg aatggttgag cctggaaagg gattggtgaa gtatggttta tgatccaaaa
     1681 cacatcatcg gtcagcgagt aaacccagga gtgttgtggt gaggtattca actggttgtg
     1741 ctgtgcgtat ggctcataag tctgggcatc tggctcagtt ttcacagtcg attgagtggg
     1801 gtatttctca agttgccagt agatactgat aggctcctcc tcgcggtctt tggtctgtgg
     1861 gtcatttcgg tggtgcttcc acttgtgatg tcatttctg
//
LOCUS       DAOQDO010001376         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_61982_length_1897_cov_2.807275, whole genome shotgun sequence.
ACCESSION   DAOQDO010001376 DAOQDO010000000
VERSION     DAOQDO010001376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61982_length_1897_cov_2.807275"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..1844
                     /locus_tag="PKZ53_19015"
     CDS             408..1844
                     /locus_tag="PKZ53_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42587.1"
                     /translation="MAQSLTSVKVLEDRLHRSPRRITLSEAAALTGLSVRDAEHALRD
                     LLVRYECRLQVTENGDLIYDFGETLNRRGEKTTAEQLEEVLDWLWNAFTVAYKTGIAV
                     TLVVYCIIFAVLLIGLMLREGGKRHGSAGIQALVRIFAAIFEWSAHSRIKHEQTRKRE
                     ADGKPKKEFIPSVYDFVFGPPREEPDPLANLRKVSAYLRQNKGVVVTSELKALLGMES
                     QEAAAFLAECVARFQGDLNVSDTGVMYAQFDQMLRTINPADTTSPQFCWETEEAPYEL
                     TGNSPGYNWLIGGMNAFNLVMSFALMVGLVVAIPPVGILLGWVPHVFSALFFVIPAVR
                     SVFVRQAEQQRQFTAARNRVMRVIFEQGGAPMLAEEMVRAVNIDRRQSPLSFEKVTEI
                     LEHLVKELPGERQFPGDGRILYAFPAIQLELNAIAQVRQSLSRDDSLGQVVFDSNVEP
                     TLEPALEPEKQMGQSVKTAPNVRRKEQL"
BASE COUNT          397 a          462 c          535 g          503 t
ORIGIN      
        1 tttcataatc acgcatgttc gcgccaaaca gaatgtcgcg aactttatcg aggcttccgc
       61 cggcgcccat taaatccatc gtggcctggg cgcgggccgc atcttccgcc gcatttctgg
      121 cttcttcccc ggcaggtcga gtcttctttt tttcttcgct ggtgctcatg caaggattct
      181 ccttgggtta agttcaggat gaaacgtggg tggattcggg agtggtcagg aatgaccgtt
      241 atgactggct tgaatgaata ggactgtgat ttcttattac catacaccta ttgcaattct
      301 gttgtgaatg gttttgaccc tgacggattg cattttctct cagaatttct tgagaacccc
      361 aaaactgcgc ttctttttca ctttttcttc tctttggcgg acactctatg gcccaatctc
      421 tgacttcggt gaaggtactt gaagaccggc tccatcggtc accgcggcgc attaccctgt
      481 ctgaagccgc ggctttgacc gggctttcgg ttcgcgatgc agagcacgcg ttgcgtgatc
      541 tgctggtgcg ctatgaatgc cggttacagg tcacggaaaa cggcgacctg atttatgact
      601 ttggcgagac tctcaaccgg cgaggtgaga aaacgacggc tgagcagctt gaagaagtcc
      661 tcgactggct ctggaacgca ttcaccgtgg cttataaaac tggaattgcc gtcacgctgg
      721 tggtgtattg catcattttt gccgtgttgt tgattgggtt gatgcttcga gaaggcggaa
      781 aacggcatgg atcggcaggc attcaggcgc tggtgcgaat ttttgcagca atttttgagt
      841 ggagtgccca cagtcgaatc aaacacgaac aaacccggaa acgagaagcc gacggaaagc
      901 caaagaaaga gtttatccct tcagtctatg actttgtgtt tgggccacca cgcgaagaac
      961 ccgacccgct ggccaatctg cggaaagtat cagcttatct tcggcaaaat aaaggtgtgg
     1021 tggtcacttc agagttaaag gcccttctgg gcatggaatc gcaggaagcc gctgcttttc
     1081 tggctgaatg cgtggcccgg tttcagggtg acctcaacgt gagtgatacc ggtgtgatgt
     1141 atgcccagtt tgaccagatg ttgcgcacca ttaacccggc agatactacc agtcctcaat
     1201 tttgctggga gaccgaagaa gctccgtatg aattgaccgg aaacagccct ggttacaact
     1261 ggctcatcgg gggaatgaat gcctttaacc tggtgatgtc gtttgctttg atggtggggt
     1321 tggtggtggc gattccaccc gttgggattt tgctcggctg ggttccgcat gtgttttcag
     1381 ccctcttttt tgtcattcca gccgtacgct cggtttttgt ccggcaggcc gaacagcaac
     1441 gccagtttac agcggcgcga aatcgagtga tgcgagtgat ttttgaacag ggtggagccc
     1501 caatgctggc cgaagagatg gttcgggctg tgaatattga ccgccgccag tcgcccttat
     1561 cttttgaaaa agtcactgag attttggagc atctggtgaa ggaactcccc ggtgaacgtc
     1621 aatttccggg tgatggccgc attctgtatg cctttccagc cattcagtta gaactcaatg
     1681 cgattgccca ggttcggcag agcctgtcac gtgatgattc gctgggtcag gttgtgtttg
     1741 attccaacgt cgaaccgacc cttgaacccg ccctggagcc agaaaaacaa atggggcagt
     1801 cggtgaagac tgccccaaac gttcgcagga aggaacaact ttagggggtt ccgggttccg
     1861 ggttccgggt tccgggttcc gggttccggg ttccggg
//
LOCUS       DAOQDO010001377         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62048_length_1896_cov_2.633895, whole genome shotgun sequence.
ACCESSION   DAOQDO010001377 DAOQDO010000000
VERSION     DAOQDO010001377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62048_length_1896_cov_2.633895"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKZ53_19020"
     CDS             complement(<1..413)
                     /locus_tag="PKZ53_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42588.1"
                     /translation="MSDILTSLRQPLSILLFCGLAGTGSMLVMASSDLTTLPVVKVQE
                     TTPSQQPPAQQPAKPRKKVFTNEDFGAPPAEEAKEQMYKKASAERAGEEEAWRAEVQA
                     AREALKSAQEQKNAPKEVTPEAIAEPKSNIDPDIQ"
     gene            complement(559..>1896)
                     /locus_tag="PKZ53_19025"
     CDS             complement(559..>1896)
                     /locus_tag="PKZ53_19025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42589.1"
                     /translation="IFEIPLWLVSSLLVFWIAKQALGFLRQPSENDDPSLLTDTSEVR
                     FVMESYQQMIDRLQVKGKELERQREAERVRAESSERFSDRLVVSIPDALIVVSIDGLV
                     SITNLEARRLFVKSDLPTSLSDSKPVSIPFQNFFAGAPAIATLVAEGLRTGETQRSTE
                     VSAELGGKRHYLEASVSPIPGRAGTWQGVLCLVSDKTEIVELRHGLQLKETLASLGEM
                     AAGIAHEFKNSLATISGFAQMLEVDADARSARPARALRTEVTHLTQVVTDFLSFARPQ
                     DTQFYPVDLQELLEACCERLRTEAERRRVQLVLTGNFPTVPGDATLLGRAFLNLIQNG
                     IEAISDEAIDREVRITHDPIDPGDETTQFCRITVTDTSSGIPASDLASVFIPFFTTKS
                     RGYGIGLAIVQKVFVGHNGRVEVASQPGHSTTFTCYLPCNQTFSSQSSVVSRQ"
BASE COUNT          456 a          476 c          500 g          464 t
ORIGIN      
        1 ttctgaatat caggatcaat gttggatttt ggctcagcaa tggcttctgg tgtcacttct
       61 ttgggggcat ttttctgctc ctgggctgat ttcaatgcct cccgcgctgc ctgcacttcg
      121 gcacgccagg cttcctcttc accggctcgt tcggcagatg ctttcttata catttgctct
      181 ttggcctctt cagccggagg ggcaccaaag tcttcgttgg taaacacttt cttccggggt
      241 ttggctggtt gctgagctgg cggttgttga gaaggggtag tttcctggac tttaacaact
      301 ggcagagttg tcaggtcact actggccata acgagcattg aacctgttcc agctaacccg
      361 cagaacaaca aaattgagag cggttgacgc agtgaagtga gtatgtccga catacaaagg
      421 acctccaaaa tcaagaagat aaaatggccg cgtagctttg aatcaggagt cagtcatcca
      481 caaagttgat tcaatgactt gagtatttcc gaatgcgatg ggttgatttt aaactgatag
      541 ccgtaggagt ttggcaatct actgacgact gacgactgac gactgacttg aaaaagtttg
      601 attgcagggc aagtaacacg tgaaggtcgt gctgtgccct ggttgactgg cgacttcgac
      661 ccgtccattg tgacctacaa acactttttg cacaatagcc agcccaatgc cgtaaccacg
      721 ggatttagtt gtaaaaaaag gaataaaaac actggctaga tcagaagccg gaatccccga
      781 gctggtatcg gtgacggtga ttcggcaaaa ttgagtggtc tcatcgcctg ggtcaatcgg
      841 gtcgtgtgtg atgcgaactt cccgatcaat tgcttcatcc gaaatggctt caatgccatt
      901 ttgaatcaaa ttcaaaaatg cccggccaag gagcgtggcg tcgcctggaa cggttgggaa
      961 gtttccggtg agcacaagct ggactcgccg ccgttcggct tcagtgcgaa gtcgttcgca
     1021 acaggcttca agcaattcct gcaaatcaac cggataaaac tgggtatcct gggggcgggc
     1081 aaaggacaga aaatccgtta ccacctgggt gagatgggtg acttcggtgc gcagtgcccg
     1141 ggcgggtcga gctgaccgcg catcagcatc aacttcaagc atttgggcaa atccggaaat
     1201 tgtggccagt gagttcttga actcatgggc aattccggcg gccatttctc ccagactggc
     1261 cagtgtttct ttgagctgca gcccgtggcg gagttcgaca atttcggttt tgtcgctcac
     1321 caggcacagc actccctgcc aggtgccggc ccgacctgga atcggcgaaa cgcttgcctc
     1381 caggtaatgc cgttttccac ccagctcagc gctgacttcg gttgaccgct gggtttctcc
     1441 ggtgcgcaat ccttccgcca caagggtggc aatggctggt gcccccgcaa agaaattctg
     1501 aaatggaatt gaaacgggtt tggagtctga cagcgacgtc ggaaggtcag atttcacaaa
     1561 taaacgtcgg gcttcgagat tggtaattga aaccaggccg tcaatcgaaa ccacaatcaa
     1621 ggcatctggg atgctgacca ccagtcggtc actgaagcgc tcgcttgatt cagcccggac
     1681 gcgctctgct tcacgctgac gttccagctc ttttcccttt acctgaagcc ggtcaatcat
     1741 ttgctgatag ctttccatca caaaacggac ttcagaagta tcggtcaaca gggatggatc
     1801 atcattctca gatggctggc gcaaaaatcc aagtgcctgt ttggcaatcc agaaaacaag
     1861 caaactacta accagccaga gcgggatttc aaaaat
//
LOCUS       DAOQDO010001378         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62090_length_1895_cov_3.692391, whole genome shotgun sequence.
ACCESSION   DAOQDO010001378 DAOQDO010000000
VERSION     DAOQDO010001378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62090_length_1895_cov_3.692391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..456)
                     /locus_tag="PKZ53_19030"
     CDS             complement(88..456)
                     /locus_tag="PKZ53_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNJ42590.1"
                     /translation="MKERQDLKKRTREFALRIIRLFAALPGSVEAQVLGKQVLRSGTS
                     VGAQYRESVRAKSDADFVSKIEGALQELEETQYWLELIGDAEIISVQRLTPLIQEAEE
                     LTAIFVSMVKAVKNKPTSRH"
     gene            complement(488..1156)
                     /locus_tag="PKZ53_19035"
     CDS             complement(488..1156)
                     /locus_tag="PKZ53_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNJ42591.1"
                     /translation="MEPAGAFALAVVTLRPEQSVKAEAGAMVSMSANVDLQSQAQGGI
                     MGVLKRAVVGESAFINTFTARGGPGEVTFAPPAPGDIVVLELVNQSYFVQSGGYLASS
                     PAIELDLKFGGAKSFFSGEGLFLMKLSGTGTLLISSFGAIRRKRLNPGERYIVDTGHI
                     VAFETSVQYTIRKASQQGFFRSMVSGEGFVCEYTGPGEIFLQTRNLSAFAGALKAFFP
                     TSGG"
     gene            complement(1440..>1895)
                     /locus_tag="PKZ53_19040"
     CDS             complement(1440..>1895)
                     /locus_tag="PKZ53_19040"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNJ42592.1"
                     /translation="QPDPFEPLEFNLSHSGDMILCAFALDREVGIDVEHIRGDFEGER
                     IANDLFSTCEKLMLAAMPEPQRRESFFVCWTCKEAYIKARGMGLSMPLNEFDVSSAMD
                     EGKTVMYDSTCDKPRWWVRRFSPGPHYAGAVAGEGRYCQFQFYDFQPEF"
BASE COUNT          489 a          513 c          452 g          441 t
ORIGIN      
        1 cgaatcggat tcttttgggg atgaaaccaa aggatgaggg atgagggatg agggatgaaa
       61 ccaaggcatg aaatcaagaa ccagtcatca gtgtcgtgaa gtgggtttgt ttttaaccgc
      121 ttttaccatt gaaacaaaaa tagcagttaa ctcttcagct tcctgaatca acggagtaag
      181 acgttgtaca gatattattt ctgcatcacc aatcagttcc agccagtatt gagtctcttc
      241 caattcctga agtgctcctt cgatcttgct cacaaagtca gcatctgatt tggctcgaac
      301 tgattcacgg tactgtgctc ccacagaagt ccctgaccgt aaaacttgtt ttcccaatac
      361 ttgagcctca actgacccgg gcagggcagc aaacaaccgg attatccgta gagcaaactc
      421 tctggttcgt ttcttcaaat cttgtctttc tttcatccct catcccttat ccctcatccc
      481 tcaagaatta tcctccgctg gtggggaaaa aggctttcaa ggcaccagca aaggctgaca
      541 ggttgcgggt ttgcaggaag atctcgcctg gcccggtata ttcacagaca aacccttcgc
      601 cactgaccat gcttcggaag aatccttgtt gtgaagcctt gcgaatggtg tactgcacac
      661 tcgtttcaaa cgctacgatg tgcccggtgt ctacaatgta gcgttcccct ggattcagcc
      721 gtttacgccg gatggcacca aaacttgaaa tcaacagggt gccggtgcct gataacttca
      781 tcaggaagag tccttcacct gaaaaaaatg atttcgcccc gccaaatttc agatcgagtt
      841 caatggctgg tgatgacgcc agatacccac ctgattgcac aaaatatgac tggttcacca
      901 gttcaagcac cacaatgtcg cccggagccg gaggggcaaa agtgacttcg ccagggccgc
      961 cgcgcgcagt aaacgtgtta ataaaggctg attcaccaac cacggcccgc tttaacaccc
     1021 ccataatgcc gccctgtgcc tgcgactgaa gatcaacatt ggctgacatg gaaaccatgg
     1081 cgccagcttc agctttcaca gattgttcgg gccgaagcgt caccaccgcc agcgcaaacg
     1141 caccagcggg ttcaatcgaa cattgaaaac cacctccgcc tgactgatac ccacctggcc
     1201 cagatggtgg ttgatatcct ccctgtggtg gataggcagg tggtggctgc ggaggcggat
     1261 accctgccgg cggcccttgt tgatacggag gaggaacata ccctccaggc tgatacccag
     1321 acggaggttg aggggtggcc ggggttggat tggccggggt gccacactgc acgcaaaacc
     1381 gatatccttc aggtacattg gcattgcatt tcgagcaaat cattcaaatg cctccattct
     1441 caaaattcag gttgaaaatc ataaaactga aactggcagt accgaccttc accagctact
     1501 gcgcccgcat aatgtggccc aggagaaaac cgacgcaccc accaccgtgg tttgtcacag
     1561 gtgctgtcat acatcacggt tttcccttca tccatggctg aagacacgtc aaattcgttg
     1621 aggggcatcg agagtcccat cccacgggct ttgatatagg cttccttaca ggtccagcag
     1681 acaaaaaacg attcgcggcg ctggggttca ggcatggcgg ccagcatcag tttttcacag
     1741 gttgaaaata agtcattggc aatccgttcg ccttcgaaat caccccgaat atgttccaca
     1801 tcaattccaa cttcgcggtc cagcgcaaac gcacacagga tcatatcccc agaatgagac
     1861 aggttaaact caaggggttc aaatggatct ggttg
//
LOCUS       DAOQDO010001379         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62177_length_1894_cov_2.506253, whole genome shotgun sequence.
ACCESSION   DAOQDO010001379 DAOQDO010000000
VERSION     DAOQDO010001379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62177_length_1894_cov_2.506253"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PKZ53_19045"
     CDS             complement(<1..1118)
                     /locus_tag="PKZ53_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNJ42593.1"
                     /translation="MPGVAQIQPTSLSPFQTAKPFERMVGYLRRYPYKLTIGCLCVLL
                     SAYIGLFGPRVIRSAIDDLQHRITREKIVLYALLIVAVSLGKGIFLFWQRQILVALSR
                     DIEFDLRNDIYAHLQRLPMAFYHENRTGDLMSRATNDIGNVRMLLGPAIMYGLNTVFV
                     TCFALPAMGHISWKLTLLALATLPLASVATNFFGSRIHKRSEEIQEYFGIITAKAQEN
                     FAGVRVVRAYAQEEAEKESFKSLNREFVSRNLRLIKLTALFSPTLHALIGFGPALVLW
                     YGGHLVLDQQITIGQFVEFNLYLMMLIWPMIALGYVVSLFQRGMAGMKRLTTVLNQEP
                     TIRDEVEPTAVTEIKGTIEFRNLNFAYPSSGPYDCRVT"
     gene            complement(1106..>1894)
                     /locus_tag="PKZ53_19050"
     CDS             complement(1106..>1894)
                     /locus_tag="PKZ53_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNJ42594.1"
                     /translation="ADIVDVNCGCPARKVVHRGAGSSLLKDLPHLEKILKGMRKAIKI
                     PLTLKMRIGWDDSSIVAVEVGKLAQDCGVEALAIHGRTRVQGYSGQADWNVIAQVKEA
                     VSIPVMGSGDVTTIDQALTRFRETGVDGVMIGRGAMANPWIFRQIDDVMQGRSPFQPT
                     LIDKRNLLFGYFDVMLKELPSELAAMGKVKQLCGQFTKGLPGGAAFRQQVFHSQSRQE
                     LTDKIDFYFTLMAEREAAGNLVPAPEDAADDTDSPTTEEYACQA"
BASE COUNT          465 a          524 c          483 g          422 t
ORIGIN      
        1 aacgttacac ggcaatcata aggcccggac gacggatagg caaaattcag gttgcgaaat
       61 tcgatagtgc ccttgatctc agtaaccgcc gtcggctcaa cttcgtcccg aatggttggc
      121 tcctggttga ggactgtcgt caggcgcttc atcccagcca tgccacgttg aaacaggctg
      181 accacatacc caagcgcaat cattggccag atcagcatca tcagatagag gttgaattca
      241 acaaattgcc caatcgtaat ctgttgatcc agaaccagat ggccaccata ccacaggacc
      301 agtgccgggc caaagccaat caaggcgtgc agggtcgggc taaacaacgc tgtcagtttg
      361 atgaggcgca gattgcgtga cacaaactcg cgattgaggc ttttaaatga ttccttttcg
      421 gcttcctctt gagcataggc ccgaaccacc cgcacgccgg caaagttctc ctgggcttta
      481 gccgtaatga tgccgaaata ttcctggatt tcttcagagc gtttgtggat gcggcttcca
      541 aagaaattgg ttgccaccga cgccagcggc aaggttgcca gggccaacaa agttagtttc
      601 cagctaatat gacccatggc tggtaaggca aaacaggtga caaaaacggt gtttaaccca
      661 tacataatcg ccggcccaag caacatccgc acattgccga tgtcgttggt ggcccgcgac
      721 atcaaatcac cagttcgatt ttcatggtaa aaggccattg gcaggcgttg cagatgggca
      781 tagatatcgt tgcggagatc aaactcaatg tcgcgtgaca gagcgaccag aatttgccgt
      841 tgccagaata aaaagatccc ttttcccagc gatacggcca caatcagcag ggcataaagc
      901 acaatttttt cacgagtgat gcgatgttgc aggtcatcaa tggcgctgcg aatcacccgt
      961 ggcccaaaaa gcccaatgta ggctgacagc aagacacaca gacagccaat ggtcagttta
     1021 tacgggtatc ggcgcaggta ccccaccatt cgttcaaatg gtttggccgt ttgaaagggg
     1081 ctgaggctgg tcggttgaat ctgggctacg cctggcatgc atattcctcg gtggttggcg
     1141 aatcggtatc atcggcagca tcttccggag ctggcaccag attgccggcg gcttctcgtt
     1201 cagccatgag cgtgaaatag aagtcaattt tgtcagtcag ttcctgtcgt gactgagagt
     1261 gaaagacctg ctggcggaac gccgcgccgc ccgggagccc ttttgtaaat tgaccgcaga
     1321 gctgttttac tttccccatc gccgccagtt cagacggcaa ttctttcaac attacatcaa
     1381 agtagccaaa caagaggttt cgcttatcaa tcagggttgg ttgaaatgga gaccgtccct
     1441 gcatcacatc gtcaatttgt cggaaaatcc acggattggc catggcgccg cgaccaatca
     1501 tcacgccgtc aacgccggtt tcgcgaaacc gggtgagtgc ctggtcaatc gtggtgacat
     1561 caccggaacc catcaccgga atactgactg cctctttgac ctgagcgatg acattccaat
     1621 cagcctgacc cgaatatccc tgaactcgcg tccgtccgtg gatcgccagc gcctcgaccc
     1681 cgcagtcctg agccagcttt ccgacttcaa ccgcgacaat tgagctgtca tcccaaccaa
     1741 tgcgcatctt cagcgtgagc ggaattttga tcgccttgcg catgcccttc agaatttttt
     1801 ccaggtgggg caggtctttg agcagcgagg aacccgcacc acggtgcacc actttgcgcg
     1861 ccgggcaacc gcaattgaca tccacgatgt cggc
//
LOCUS       DAOQDO010001380         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62232_length_1893_cov_2.621872, whole genome shotgun sequence.
ACCESSION   DAOQDO010001380 DAOQDO010000000
VERSION     DAOQDO010001380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62232_length_1893_cov_2.621872"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PKZ53_19055"
     CDS             complement(<1..1154)
                     /locus_tag="PKZ53_19055"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNJ42595.1"
                     /translation="MPIAFSRSMRSLSGDRYRRSLLGISLVMAILGAWGIWFWSARVA
                     RYETSQTARLEMTQAAAAIEASEDGRIAGLHTVAGQEVKPGQVLVELETTVDRLRLEE
                     QNRLLDSLNQEISVLRDELTAQRQAQQDDRATAPVALQVARARHREAEEDAKFSQEEV
                     TQLTKLRDGGYISNLQLLRAEADYRKHRALADALDLEVRRLELDQQTTESQKKVRLEQ
                     IKRELTKLQGEITTITATITTLQKDIEKYQIQSPVAGRVAELVPLHVGDLLRKGEKLG
                     VVLPTGELKIVAQFSPSAGFGRIRPGQKARVRVQGFPWTQYGELTATVTNVAGETRDG
                     LVRVELAILPTSSNLIPLQHGLPAVVEVEVEHLSPASLVLRSAGYGAESTS"
     gene            complement(1377..>1893)
                     /locus_tag="PKZ53_19060"
     CDS             complement(1377..>1893)
                     /locus_tag="PKZ53_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42596.1"
                     /translation="WSPFRFLLPLIPFLLFYLVHGWRVVVEWVLKEANRPAAEAQFMP
                     ARIFLLCVICLFVYDHIGYLQAKTNPSVNEGQPPVIQTFAAAEEVMEWVRTHTSETEI
                     LTTNNLPLVHIYSGRKTIACTLPDCPAQGVRYFVNVAPLTPVPSMIPGKIVFQPNHSS
                     LYVFEISKAEN"
BASE COUNT          457 a          476 c          513 g          447 t
ORIGIN      
        1 gaggttgact cggcgccata cccggcagag cgcagcacca gactggctgg tgacaggtgt
       61 tcgacttcga cttcaaccac cgccggcaga ccatgctgga gtggaatcag gtttgatgag
      121 gtcggcaaaa tcgcgagttc aacccgcacc agcccatcac gggtttcacc tgcgacgttg
      181 gtgaccgtgg cggtgagttc tccatattga gtccagggaa atccctggac ccgaactctg
      241 gctttttgac cagggcgaat ccggccaaac ccggctgacg gactaaactg ggcgacaatc
      301 tttaactcac cagttggtaa aaccacgccc agtttttcgc ctttgcgaag caaatcgccg
      361 acatgcagcg gcaccagttc ggcaacgcgt ccagccactg gcgattggat ttgatatttc
      421 tcgatatctt tttgaagtgt ggtgatggtg gctgtgatcg tggtgatttc accctgcaat
      481 ttggtgagtt cacgcttgat ttgctccagc cggacttttt tctggctttc agtggtttgc
      541 tggtcaagct ccaggcggcg aacctccagg tccagggcat cagccagggc acgatgtttc
      601 cggtaatcgg cttcggcccg caggagttgt aaattggaaa tatagccacc atcgcggagt
      661 ttggtgagct gggtgacttc ttcctggcta aatttggcgt cttcttcagc ttcccggtgc
      721 cgggcgcgag cgacctggag cgcaactggc gctgtggccc ggtcatcctg ctgggcctga
      781 cgctgggcgg tcagttcatc tcgcaacacc gaaatttctt gattgagcga atccagcaac
      841 cggttttgct cttcaagccg caaccgatca acggtggttt caagttcaac cagtacctgt
      901 ccgggtttga cttcctggcc agcgacagtg tgaagcccgg cgattcggcc atcctccgaa
      961 gcttcaatcg ccgcggccgc ctgggtcatt tcaagccggg cggtttgcga agtttcatag
     1021 cgggcaacac gggcagacca aaaccagatg ccccaggcac ccaaaatcgc catcaccagg
     1081 ctaataccca gcagcgagcg gcgataccga tctccggaaa gagaccgcat tgatcgggaa
     1141 aaagcaatcg gcatggaaat aaagcgtcag gaataaaaat gaagacaccg aatggagggc
     1201 agagtacggg atgattggaa aatggtctac agtttttttg aaacaaacaa aaaggcgaac
     1261 cagagcggat tcacctgtgg cgtgctggat ttatttttct tcatcgggtg aagaagttcc
     1321 ctggctcatt accgtttcaa gatggctgtg acctttgggg actcaatcgc gcaacatcaa
     1381 ttctcagcct ttgagatttc aaacacatag agcgatgaat gattgggttg gaaaacaatc
     1441 tttcctggaa tcatacttgg aaccggagtc agtggagcta cattgacaaa atagcgaacc
     1501 ccttgcgccg ggcagtctgg caaagtacag gctatcgttt ttcgaccgct atatatgtga
     1561 acaagtggca aattgttagt tgtcagtatt tctgtttcag aagtatgtgt tctcacccat
     1621 tccatgacct cttcggctgc ggcaaatgtt tggattacag gcggttgtcc ctcgttaaca
     1681 gatggatttg ttttcgcttg cagatatcca atatggtcat acacaaaaag gcagatgaca
     1741 cagagcagga agattcgggc tggcataaat tgggcttcgg ctgccgggcg gtttgcctct
     1801 ttgagaaccc attccaccac cacacgccaa ccgtgaacga gataaaataa taaaaatggg
     1861 atgagaggta agaggaatcg aaacggagac cag
//
LOCUS       DAOQDO010001381         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62281_length_1892_cov_2.967882, whole genome shotgun sequence.
ACCESSION   DAOQDO010001381 DAOQDO010000000
VERSION     DAOQDO010001381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62281_length_1892_cov_2.967882"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKZ53_19065"
     CDS             complement(<1..313)
                     /locus_tag="PKZ53_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42597.1"
                     /translation="MPPLRKTQRNNHFTVSNQKRNIKGTLFLLLQIGIVLFLGSILPV
                     STNFLPVSNAQSGNTLDKVLSCGKPGEAGGIAPGADLYRINLDLTKFPGAICNDGTGG
                     VF"
     gene            804..>1892
                     /locus_tag="PKZ53_19070"
     CDS             804..>1892
                     /locus_tag="PKZ53_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNJ42598.1"
                     /translation="MAHTLFNSLQEFTLGNGSTGKYYSLPALEAAGVGPISKLPVSIR
                     IVLESVLRNYDDLKIKESDIRTLANWQAKAERTAEIPFVVARVLLQDFTGVPLLVDLA
                     AMRSAMSQLHKNPKAIEPLVPVDLVIDHSVQVDYTGPTAARRLKTLTEENGSGLPATD
                     DAFQLNMMLEFERNRARYQFLKWGMQAFESFKVIPPGIGICHQVNLEYLAKGVLEREG
                     VYYPDTLVGTDSHTTMINGLGIVGWGVGGIEAEAGMLGQPVYFLTPDVVGVHMHGKLR
                     EGVTATDLVLHVTEMLRKAKVVGKFVEYHGEGAANLSLTDRATISNMGPEYGATMGYF
                     PIDEETCKYLLATGRSPEHVETFRNYYTA"
BASE COUNT          452 a          484 c          494 g          462 t
ORIGIN      
        1 aaaaaacccc gccagtaccg tcattgcata tggcgcccgg gaattttgtc aggtcgaggt
       61 taattcgata gagatcagct ccaggagcga tgccgccagc ctcaccaggt tttccacagg
      121 acagcacctt atcgagggta tttcctgatt gcgcgtttga aacgggaaga aaattggttg
      181 atacaggaag gattgaacca agaaagagta caatccctat ctgtaataaa agaaagagcg
      241 tacctttgat atttcttttc tgattcgata cggtgaaatg attgttccgt tgtgtttttc
      301 gaaggggggg catagggtta gctcctcaac tggtgtcaga ttgtacacat ttatggggca
      361 ttggacgccg gtttcctgaa aaagtcttaa aatacagggc ggaagacatc gggcggaaga
      421 cattgggctt ggggctgaag actcgcaaag ctcggagcaa ttgaagggat gagggatgaa
      481 ataaaaccaa ttcttcagcg cattttcttc agcccgtttt cttcagcccg tcttcttcag
      541 cccgttttct tcagcgcatt ttcttcagcc cgttttcttc agcccgtttt cttcagcccg
      601 tcttcttcag cccgttggct tccacaccat gcctgttccc catcgcccaa aaatgtgaca
      661 caatcgcccg gcaatgtttc tgcttttcct tcatttctgc agtctgcaat caaaccaatc
      721 aggaaaacac tggacctgag ccagccggct tggggtctgt ctgttttcta atctttcagc
      781 agggaaaaca accacgaacg tctatggcac atactctttt taactcattg caagaattca
      841 ccctgggcaa tggcagcaca gggaaatatt actcgttacc ggcactcgaa gcggctgggg
      901 tcggccccat ttcaaaattg ccggttagca ttcggatcgt gttggaatca gtcctgcgca
      961 attatgacga tctgaaaatc aaagaatccg acatccgcac gctggcaaac tggcaggcca
     1021 aagctgagcg gacggctgaa attccgtttg ttgtggcccg tgtgctgttg caggatttca
     1081 ccggtgttcc gttgctggtt gacctggcgg ctatgcgatc agccatgagc cagttgcaca
     1141 aaaatccgaa ggcgattgag ccgctggtcc cggttgatct ggtcattgac cactccgtcc
     1201 aggtggacta taccggtcca accgctgccc gccgattgaa aacactgacc gaagaaaatg
     1261 gatctgggct tcctgccacc gatgatgcct ttcagctcaa catgatgctg gaatttgagc
     1321 gcaaccgggc ccgctatcaa tttttgaagt ggggcatgca ggcgtttgaa agcttcaaag
     1381 tcattccgcc agggattggt atttgccatc aggtcaattt ggaatatctg gccaaaggtg
     1441 ttctggaacg cgaaggcgtg tattacccgg acacactggt tggaactgac tcgcacacca
     1501 ccatgatcaa tggcctggga attgtcggct ggggcgtcgg cgggattgaa gccgaagccg
     1561 gaatgctcgg ccagccggtg tacttcctga cgccggatgt ggttggtgtc catatgcacg
     1621 gcaagttacg cgaaggcgtc acggccactg atctggtgct gcatgtgacg gaaatgctcc
     1681 gcaaggccaa agtggtcgga aaatttgtcg aatatcacgg cgaaggcgcc gccaatctct
     1741 cgctcaccga ccgggccact atcagcaaca tgggccctga atacggcgca acgatgggct
     1801 acttcccgat tgacgaagaa acctgcaagt atttgctggc gacgggacgg tcacccgaac
     1861 acgtcgagac attccgcaat tactacaccg ct
//
LOCUS       DAOQDO010001382         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62328_length_1891_cov_3.772876, whole genome shotgun sequence.
ACCESSION   DAOQDO010001382 DAOQDO010000000
VERSION     DAOQDO010001382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62328_length_1891_cov_3.772876"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKZ53_19075"
     CDS             complement(<1..832)
                     /locus_tag="PKZ53_19075"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42599.1"
                     /translation="MMYHGRLFLLAGFVCLALTIMPGFAQENSWEKYHQIGKKAFEQN
                     QFEEAEKALLVAVKESESFPAPDTRLAQSLTCLAQCYQAKGKFPEAEVAYERALTLWK
                     ERRGAGHPTVAAVSSNLASLYNARGKYSEAEKMYSQALAGLEASDKPDVANLANTMNN
                     LAELYRTQSKYTQAEPLYNRAIRLLEEKFGLEDPKVSTPLNNLGTMYIEMSETLEAEK
                     HCKRALAIREKAFGPEHPMVATTLNNLAACYKVRGKTTLAEEYYNRALVIWEKTIGPE
                     H"
     gene            1188..>1891
                     /locus_tag="PKZ53_19080"
     CDS             1188..>1891
                     /locus_tag="PKZ53_19080"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNJ42600.1"
                     /translation="MHVKLALIGLGNIGRRFLELLERKQELLSTRFGLELSVVSVADS
                     SGARHYPDGANLRQLIEMKLAGQRLKDHHEALIDPLDMVRQTEANVLIELSPTNLQTG
                     GTGLSAIRIALERNLHVVTANKGPLVLAFQELVTKAREQQVRLLFSATVTGGLPTLNV
                     GRRDLSACQIKKFEGIVNSTTDFILTCMSEGETFDQGLKRAQDAGFAEADPTLDVDGW
                     DAANKLVIIANTVLRRP"
BASE COUNT          440 a          463 c          467 g          521 t
ORIGIN      
        1 ggtgctctgg accaatggtt ttctcccaaa taaccagcgc ccggttgtaa tactcttcag
       61 ctaaggttgt ttttccacga actttatagc aggcagccag attattgagt gtggtggcaa
      121 ccattgggtg ttctggtcca aacgcctttt cccgaattgc cagcgcccgt ttgcagtgct
      181 tttcggcttc gagggtttca ctcatttcaa tatacatggt gcctaaatta ttgagcggag
      241 tggaaacctt gggatcttcg aggccaaatt tttcttcaag caggcgaatg gcgcgattgt
      301 acagtggctc ggcctgggtg tacttactct gggtgcgata gagttcggcc agattgttca
      361 tggtgtttgc caggttggca acgtcgggtt tgtcactggc ttcaagcccc gccagagctt
      421 gtgagtacat tttttcagcc tcactgtatt tcccgcgcgc gttatacaaa ctggccagat
      481 tgcttgatac ggctgccact gttggatgcc ccgccccacg ccgttctttc cacaacgtca
      541 aagctcgttc atacgcaact tctgcttctg gaaattttcc tttggcctga taacactgcg
      601 ccagacaggt caaactttgg gccagtcggg tatcaggcgc cggaaaggat tcactttctt
      661 tcactgcgac cagaagcgct ttttcagcct cttcaaactg attttgttca aatgcttttt
      721 ttccgatttg gtggtatttt tcccaggaat tttcctgagc aaacccaggc attatagtca
      781 aggcaagaca cacaaatccc gccagaagaa agagtcttcc gtggtacatc atcttggaat
      841 atcctccaga aacctagatt ttattacagt ttccaagaga gcttggttgt ggcctggatt
      901 tgaaaaacga ttgtgggagt ggatgtcgcc ttaaactgac tgaactcgat caaagttcca
      961 attatttctg aattctttta ccttttctta atatcttttt tcttgaatat tcaagattct
     1021 cattggaatt aatttcattt ttttgaagaa caaaagctga cgtcatattc acttttcagc
     1081 ttttcggttg tcagaggtaa gccggacatg gatagtgtgg cggcatttcg ataccaccat
     1141 ggctctcgct ctggagccag acttttttaa attcacacga ggaacgcatg cacgtcaaac
     1201 tggcactcat tggacttgga aatatcgggc gacgcttttt ggagttgctc gaacgcaaac
     1261 aggaactgct gtcaacgcgg tttggacttg aactttcagt ggtcagcgtg gctgatagca
     1321 gcggggccag gcactatcca gatggtgcca acctgcgcca gttgatcgag atgaaactgg
     1381 ctggccagcg actcaaagat catcatgaag cactcattga cccgctcgat atggttcggc
     1441 aaaccgaagc caacgtgttg attgagctat caccgacaaa tttgcagacc ggcgggacag
     1501 ggctttctgc gattcggatc gcccttgaac gaaacctgca cgtcgtgaca gccaacaaag
     1561 ggccgctggt cctggccttt caagaactgg tcaccaaagc ccgtgaacaa caggtccggc
     1621 tgctttttag tgcgaccgtg acgggcggcc tgccaaccct caatgtcggg cggagggact
     1681 tgagcgcctg ccagattaaa aagtttgaag gcatcgtcaa ctcaaccacg gattttattc
     1741 tgacctgtat gtctgaaggg gaaacctttg accaggggtt aaaacgcgcc caggatgctg
     1801 ggtttgccga agctgacccg accctggatg tggatggctg ggatgccgcc aataaactgg
     1861 tcatcattgc caacacagtt ttacggcgac c
//
LOCUS       DAOQDO010001383         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62368_length_1891_cov_2.050654, whole genome shotgun sequence.
ACCESSION   DAOQDO010001383 DAOQDO010000000
VERSION     DAOQDO010001383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62368_length_1891_cov_2.050654"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1891
                     /locus_tag="PKZ53_19085"
     CDS             <1..>1891
                     /locus_tag="PKZ53_19085"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HNJ42601.1"
                     /translation="QACLRTVETWMVRSLPIPTPVIQALWEDSAWKKVLENTVVMPVD
                     DTGQVRFELAGLLRGSDLQKGLGLVNLDGETGWTSLPAIVIPHPILLPELNDFRELLV
                     ELNIKQGIEQVFREIWIRPADLPPQVKRLDTYANGKFTKFGYVWGRCRKLGFRLQAGQ
                     ARCPVWENGHLIEARYTVGDASGSWIVTGNLEWVDEQGRNLKVADLGPVAFSEGSRMA
                     ALIYAARKIDASDTSPSQLTPGQSQTSGTIRKTSHSMALSPEAALHAGGLILPGATVR
                     DSVRVDGVSARRYRHRALPGRVITRLVADILAPGVDREMEVFGFDLPEVLPGIAQQRR
                     RAIGFPGWALIHDLEHASFALDVVKEMRQHAKTAQLKKNAAMEGFENLGIRLGRSVPH
                     FLPSYFEEVGRIFIELGSPTLGAQYFQKARQAEEIYALPIDDAQHQETFLEFALAGAL
                     RATALSKYTDQLGKKYGPARAYESFRELCIKRIIGGLPPWGGMAKRLRSLAKDAGLDV
                     EQEDQILLEQVLESSVMHRAPLEFWRAYRPSLVTIAQNSPRVRGFLLNLFPGSAYASM
                     EFHIWWFEFLDDCGALEALWLPPESVLPETAPDDGAAGWFKQALTQSSYGWKQYIPLR
                     LFQLL"
BASE COUNT          414 a          482 c          555 g          440 t
ORIGIN      
        1 aacaggcgtg cctgcggacc gttgaaacgt ggatggtgcg gtcattgccc atccccacac
       61 ctgtcatcca ggcactgtgg gaagactcag cctggaaaaa agtccttgaa aacacagtgg
      121 tgatgccagt tgatgacact ggccaggtgc ggtttgaact ggcggggtta ttgcgtggga
      181 gcgacctcca aaaagggctg gggctggtca acctggatgg tgaaaccggg tggacctccc
      241 ttccagcgat tgtgattcca cacccaatct tgctcccgga actcaatgat tttcgggagt
      301 tgctggtgga gttgaatatc aagcagggca ttgaacaggt tttccgtgag atttggatcc
      361 gaccggctga cctgccgcca caggtaaaac ggctggatac ctatgccaat gggaagttta
      421 ccaaatttgg atatgtctgg ggacgttgtc gaaaacttgg gttccgattg caagccgggc
      481 aggcccggtg cccggtgtgg gaaaatggac atttgatcga agcccgctat accgtcggcg
      541 atgcgtcggg aagctggatt gtgactggaa atctggaatg ggtggacgaa cagggcagaa
      601 atctcaaagt ggcggatctt gggccggttg cgttttcaga aggcagccgg atggcggcgt
      661 tgatttatgc cgcccggaaa attgatgcgt cagacacgtc accatctcaa ctcactccgg
      721 gtcagtctca aacttcggga actatcagga aaaccagtca ctcaatggca ctttcgccag
      781 aggccgcact ccacgccgga gggttgatcc tccctggagc gacggtccgt gattcggtcc
      841 gggttgacgg tgtcagtgcc cgccggtatc ggcaccgggc gctccctggg cgggtgatca
      901 cccggctggt tgctgatatt ttggcacctg gggttgatcg tgaaatggag gtgtttggct
      961 ttgatttgcc agaggttctg cccggaattg cccaacaacg gcgacgagcg attggatttc
     1021 cagggtgggc gctgatccac gacctggaac acgcctcgtt tgcgctggat gtggtcaaag
     1081 aaatgcggca gcacgccaaa accgcgcaac tgaaaaaaaa tgcagccatg gaaggattcg
     1141 aaaacctggg cattcggttg gggcgctcag tcccccactt tctgccatcc tattttgaag
     1201 aagtcggacg catttttatt gagttgggca gtccgaccct gggtgcccaa tattttcaga
     1261 aagcacgtca ggctgaagaa atttacgccc ttccgataga tgatgcccaa catcaggaaa
     1321 cgtttctcga atttgccctg gctggtgccc tgcgggccac cgcactctct aaatatactg
     1381 accagcttgg caaaaaatat ggtccggcca gggcgtatga atcttttcgt gaattatgta
     1441 tcaaacgcat cattggtgga ttgccgccct ggggtggaat ggccaaacgg ctgcgctcgc
     1501 tcgccaaaga tgccggcctg gatgtggaac aggaagatca aatcctgctt gaacaggttc
     1561 tggaatcgtc ggtcatgcac cgtgccccac tggaattctg gcgagcctat cgcccatcac
     1621 tggtcaccat cgctcaaaat tcacctcggg ttcgagggtt cctgctcaat ttatttccag
     1681 gctcagccta tgcgtctatg gagtttcaca tctggtggtt tgagtttctg gatgactgtg
     1741 gcgcgttgga ggcactctgg ctgccacctg aatctgttct gccggaaaca gcccccgacg
     1801 atggggccgc tggctggttt aaacaggcat tgactcagag cagctacggc tggaaacagt
     1861 acattccgct tcgattgttt caactgctca g
//
LOCUS       DAOQDO010001384         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62425_length_1890_cov_2.280109, whole genome shotgun sequence.
ACCESSION   DAOQDO010001384 DAOQDO010000000
VERSION     DAOQDO010001384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62425_length_1890_cov_2.280109"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1890
                     /locus_tag="PKZ53_19090"
     CDS             24..>1890
                     /locus_tag="PKZ53_19090"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42602.1"
                     /translation="MRLNQRDKALDVFQQALKVWDTNQNKLDRAITLNSLCFFYINIG
                     ELTKSATVAREAIEIFRQFQDQDGEAGTLNNLANTLTYLGEVRQAQECLNRVLAIDQA
                     NGSPFDQATTLNNLGGTYDKLGETQRARACYEQALPLRRLAKDRQGEAFTLINISVNE
                     GRLSNLQQAIQFIQQAQVILKELDDQEGQAYVLSNLAFYYKQLGDPDKTLQYMLEGAN
                     LWKKLGSQRQYANMMIALGRFYAETNDDAQAKMYYTQALDLSRPHGYASIQEYGLINL
                     GKVFLKEHDLAQAEKCFSEALAVNQKTNDLIGRAFLFDNLADLNIEKKAYPAARQYLE
                     QSLALNTKVAYRANIAENLYSLAVVDRQEGKLLDALNRMEQAIPLFEQIRSKVDDQRL
                     RTTYFSSNQIFYNFYLQLLMDLHQQFPQQGYAALALQAKERATARGLIELLRESKTDI
                     RQGVAPHLIQREAELNQQLGDKATRITEFLSRGAPSGLKESVEREFTELNAEYQQVQA
                     EIRQKSPAYAAITQNQSATLADIQTQLLDDNTQLVEFALAPEKSFVWLVSQKSIEVFL
                     LPPKEEIERCASQVYELLTTRNLPVTTTHDRKVQIAKADMAFPAAVRELSQLLFGQ"
BASE COUNT          529 a          503 c          430 g          428 t
ORIGIN      
        1 tccatcacat ggctcgggcg tatatgcgac tcaatcagcg ggacaaagcc ctcgatgttt
       61 tccagcaggc actcaaggta tgggacacca atcaaaataa acttgatcgg gcaattacct
      121 taaacagttt gtgtttcttc tatatcaaca ttggggaact gaccaaatca gccacagtgg
      181 ccagggaagc catcgaaatc tttcgccagt ttcaggatca ggatggcgag gcaggtacgc
      241 tcaataatct ggcaaacacc ctgacctatc taggtgaagt acgtcaggcc caggagtgcc
      301 tgaatcgggt cctggccatt gatcaagcca acgggtcgcc ttttgatcag gcgacgacac
      361 tgaataacct gggtggaacc tatgacaagc ttggggaaac ccagcgtgcc cgggcctgct
      421 atgaacaggc cctcccactt cgccggctcg ccaaagatcg tcaaggcgaa gcatttacct
      481 tgatcaatat cagcgtgaat gaaggcagac tctcaaacct ccagcaagcg attcagttca
      541 tacaacaagc tcaggtgatt ctcaaagaac tcgacgacca ggaaggtcaa gcctatgtgc
      601 tcagcaatct cgccttttac tacaaacaac ttggtgatcc agacaaaaca cttcagtaca
      661 tgctcgaagg ggccaatttg tggaaaaaac ttggcagtca acgtcaatat gccaatatga
      721 tgattgcttt gggccggttt tatgcagaaa ccaacgatga tgctcaggcc aaaatgtact
      781 atacccaggc ccttgacctg agtcgccccc atggctatgc atccattcaa gagtatgggt
      841 tgatcaatct tgggaaggta tttctcaaag aacacgatct ggcccaggca gaaaaatgtt
      901 tttccgaggc gctggccgtg aaccaaaaaa ccaatgacct gatcgggcgt gcctttctgt
      961 ttgacaatct cgccgacctc aacattgaaa agaaagcgta ccctgccgca cgccagtatc
     1021 tggaacaatc actggccctc aataccaagg tggcatacag ggccaatatt gccgaaaact
     1081 tgtattccct cgccgttgtt gaccgccagg aaggcaaact ccttgatgcc cttaaccgca
     1141 tggagcaggc cattcccttg tttgagcaaa tccgatcaaa ggttgatgac cagcggttac
     1201 gaacgaccta tttttcctcc aatcagattt tttataattt ttatcttcaa ctcttgatgg
     1261 accttcatca gcaattccca cagcaagggt atgctgccct ggcattacaa gccaaagagc
     1321 gggccacagc tcgcggtttg attgaactac ttcgagaatc caaaaccgat atccgtcagg
     1381 gtgttgcccc acacctgatc caacgggaag ccgaattgaa tcaacagtta ggcgacaaag
     1441 ccacccgaat caccgagttc ctcagccgtg gcgccccatc cggcttgaaa gaaagtgtgg
     1501 aacgtgaatt taccgaactc aatgccgagt atcagcaagt ccaggctgaa atccgtcaaa
     1561 aaagtccagc ctatgcggcg atcactcaga atcaatcagc gactctggcc gacattcaaa
     1621 ctcagttgtt ggacgacaat acccaactgg tggaatttgc cctggcacct gaaaaaagct
     1681 ttgtctggct cgtgtcgcaa aaatcaattg aagtttttct acttccacca aaagaggaaa
     1741 tcgaacgctg tgccagccag gtgtatgaat tgctgaccac acgcaatctg ccggtgacca
     1801 cgacgcatga ccgcaaagtc caaattgcca aagctgatat ggcttttcca gccgcagttc
     1861 gcgaattaag ccagctttta tttggccagt
//
LOCUS       DAOQDO010001385         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62460_length_1889_cov_3.580153, whole genome shotgun sequence.
ACCESSION   DAOQDO010001385 DAOQDO010000000
VERSION     DAOQDO010001385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62460_length_1889_cov_3.580153"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKZ53_19095"
     CDS             complement(<1..1208)
                     /locus_tag="PKZ53_19095"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HNJ42603.1"
                     /translation="MASRIRDCITITSYTPIDFTESGWQTPMLREALFTYVLGDILER
                     LTQHLTTPSSTPHRCAMLVGPPGSGKTYLTKLAQALLQSAGQPLSQSHSRIKEIHTRL
                     GKTRYLPVELSPTLTVGNYSSEPTLEYLKHYLHHLATSDFSSDTFDLQQALATLRDTF
                     KHFPADTRILVVIDSLEDWLKPRDRRTGQRMVNLLTLLSVASQELPLAMITNVNDRTL
                     DSVNYGWLSGEQVIQMMSCSSLEYLTGNSIPTLIGIHLLPKTARQRTTISKIREQLRQ
                     TLPELNLDEQEFVNIYPLHPAVWEIGARLRPYLEKFSFPAFAIKAAEKVRNRPAESLF
                     TVDEMLDVLESSLRRSPQLKFAFEAYDKTIESVLPRVSQGQRLHTRMLIKAILMHTLA
                     GLPATVRDLT"
BASE COUNT          485 a          442 c          490 g          472 t
ORIGIN      
        1 ttggtcaggt cccgaaccgt ggccggcaac ccggccagtg tgtgcatcaa gatcgctttg
       61 atgagcatgc gggtatgcag tcgctgcccc tgactgaccc gtggcaaaac cgattcgatg
      121 gttttgtcat aggcttcgaa cgcaaatttg agttggggag atcggcgcag tgacgactcc
      181 agcacatcaa gcatctcatc aactgtaaac aggctctcag ccgggcgatt gcgaaccttt
      241 tcagccgctt taatcgcaaa cgcaggaaat gagaatttct ccaggtatgg ccgcagccgg
      301 gccccaattt cccaaactgc cgggtggagc gggtaaatat tgacgaattc ctgctcatcc
      361 agattgagtt ccggcagtgt ctggcgaagt tgctctcgga ttttgctgat ggtggtccgc
      421 tgacgggcag tttttggaag caagtggatc ccaatcaggg tcgggatgga atttccagtc
      481 aggtattcaa gcgaactaca actcatcatc tgaatgacct gctcaccgct caaccagcca
      541 taattgaccg aatccagcgt ccgatcattg acattggtga tcattgccag cggaagttcc
      601 tgtgaagcca cgcttaacaa cgtcaataaa ttgaccattc gttgacctgt acgccggtca
      661 cgaggtttga gccagtcttc caggctatca atcacgacca gaattcgcgt gtcggctgga
      721 aaatgtttga atgtatcgcg taaggtggcc aacgcctgtt gcaagtcaaa cgtgtcactt
      781 gaaaagtcag aggtcgccag atgatggaga taatgtttga ggtattcgag tgttggctcg
      841 gaactgtagt tcccgaccgt gagtgtcggg gacagttcaa ccgggaggta ccgcgtcttg
      901 ccaaggcgag tatggatttc tttaatccgc gaatgagatt gagacagcgg ttgccccgcc
      961 gattggagca atgcctgagc aagcttggtt aaataggttt tgccgcttcc tggcggcccc
     1021 accagcatgg cacagcgatg cggggtagaa ctgggggtgg tcaaatgttg ggtcaggcgc
     1081 tctaaaatgt cacccagtac ataggtgaac aacgcctcac gcaacatcgg cgtttgccat
     1141 ccactttctg tgaagtcaat tggtgtataa gaagtaattg tgatgcagtc ccggattcgg
     1201 ctggccataa cgtgtatgtg aaaatgtcaa ctgaggtaag tttgtctgag tgccgtggaa
     1261 tcagtgtttg agccgcctgt cattgaccag agggcataac cgacctggca atgcaccagg
     1321 ccgaagtgta tgaaccagta actatccaac cctcacctgg ttcaagggtg aaggattgag
     1381 aaaacgctca ctggtggctg gattgagatc aaatttgcaa ggagtttttt attcgtgcct
     1441 ggtgttgtaa gtgtcaaaca cgtcaaaaat ctttcaagat ttcttatatc atttgagctt
     1501 aaaccaaaaa agggattggg cagtgactag ttgtgaggcg aaatccacag ggaaattcca
     1561 tcatacacca ggatttgtct gaaagaaaaa acattttctt ggcattcaaa acaaaggatt
     1621 tcaaggattt ctcccgacca gaccgaaaga aaaagctcct tgcccggatc gggaaatcca
     1681 taggcggctg attttgttct gagggggaat ccatccccac agaccgaaga aaacagtttg
     1741 gatgaccggt caatatcagt ataatgctgc ctgagcagtt ccttcacaca cgggcttttc
     1801 cccacataag tcatctcttc ccaagaattt gcagatttta gacacctttt caggacaatt
     1861 ccaatgaggg attccatcat tttctatct
//
LOCUS       DAOQDO010001386         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62568_length_1887_cov_6.197598, whole genome shotgun sequence.
ACCESSION   DAOQDO010001386 DAOQDO010000000
VERSION     DAOQDO010001386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62568_length_1887_cov_6.197598"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..162
                     /locus_tag="PKZ53_19100"
                     /pseudo
     CDS             52..162
                     /locus_tag="PKZ53_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(325..1272)
                     /locus_tag="PKZ53_19105"
     CDS             complement(325..1272)
                     /locus_tag="PKZ53_19105"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNJ42604.1"
                     /translation="MTKKAITNVPDSIRHRLLNKSRETGRPYEEFLKYYAIERFLYRL
                     GNTRQAERLILKGGLTLRIWEGAWSRPTMDIDVLAQLEGGAEAVVAMVRECLRLNDTD
                     DGLQFNEDHLRIEPIRAKKEIPGIRLLIPATLGTTRIQVQMDVGFDDEVLPPPQLLQY
                     PTLLNHPIPQLRVYPPEALIAEKFQAIVALDTANTRLKDFYDIWMLARRRNFQGDLLS
                     QAIRTTFSKRNTDLPQEVPIGLTQIFFDDAIKAKQWSSFLNQNRLPETSLKLPEVAET
                     LTLFLLPVTRGAVFSHIWNFQKVVWEPITAESPGLKDQH"
     gene            complement(1269..1859)
                     /locus_tag="PKZ53_19110"
     CDS             complement(1269..1859)
                     /locus_tag="PKZ53_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNJ42605.1"
                     /translation="MTQALDFIQQAGGTVRASQALALGIHPRTLYHLRDEGKLIELSR
                     GVYRLADLPWPDHPDLVTVATRVPKGIIGLVSALAFHNLTTRIPHWVDIMLPKGSKTP
                     RLTYPPIRAFHVSPLAYEAGIETQVVDGVSVRIYSAEKTIADGFKFRNRIGLDTVIEA
                     VQLGLEREIVTMAGILQFARICRVEKVVTPYLEALI"
BASE COUNT          453 a          474 c          475 g          485 t
ORIGIN      
        1 agggcggaaa actggatcca aagtatttaa tcacgatccc acgggaagtg tatgaaatgg
       61 aataatccat actttcgtca gatccagctt ttggtggtgg tgttaccact ggtggccaaa
      121 gaaagctgtt tggcactcaa aggcggcacc gccattaacc tgtcagcctg ttcggactca
      181 ggtgcgcctc agaccgttcg tgatcccgaa gtgagcctga attgaatcgg gagttgcgac
      241 ttttgagcgg cgaaccccgc atttccaaag actgattcca tcgacattct ttcatttttg
      301 gcggatgact gttcattttg catttcagtg ctggtccttc aatcctggcg attctgctgt
      361 tatcggctcc caaaccactt tctgaaagtt ccagatgtgg gaaaataccg ctccgcgggt
      421 gacgggcaat agaaataaag tcagtgtttc cgccacttct ggcagtttta ggctcgtttc
      481 aggaagtcgg ttttggttga gaaatgagct ccactgttta gctttgattg cgtcatcaaa
      541 aaagatctga gtcaacccga ttggaacctc ttgtggcaaa tcagtgttcc gttttgaaaa
      601 tgtggtcctg atggcctggc ttaaaaggtc accctgaaaa tttctgcggc gagccagcat
      661 ccagatatca taaaagtctt tcaatcgagt gttcgctgta tcaagcgcga cgatggcctg
      721 gaatttctcg gcgatcaagg cttccggcgg gtacacccga agttgcggaa ttggatggtt
      781 gagcaaggtg ggatattgga ggagttgggg tggcggcagt acctcatcat caaaacccac
      841 atccatttga acctgaattc gggtggttcc aagcgtggct ggtatcaaaa gtcgaatgcc
      901 cggaatttcc tttttggccc ggatcggttc aattcggagg tggtcttcgt taaattgcaa
      961 cccatcgtct gtgtcgttca aacgcagaca ttctcggacc atcgccacca ctgcctccgc
     1021 tccaccttca agctgggcca gtacatcaat atccatagtt ggtcgtgacc aggcgccttc
     1081 ccaaatccga agcgtcaacc cacctttgag aatcaaacgc tccgcctgtc tggtatttcc
     1141 taaccggtac agaaaccgct caatcgcata gtatttcaaa aactcttcat atggacgtcc
     1201 tgtttcccgg gacttgttca agagccggtg gcgaattgaa tccggtacat tggtgatggc
     1261 ttttttggtc atatcaatgc ctccagatag ggagtcacga ccttctcaac ccgacaaatg
     1321 cgggcaaact gcaaaatacc agccatggtg acgatttccc gttccagacc aagctggacc
     1381 gcctcgatga cggtatcgag tccgatccga ttgcggaatt taaacccatc ggcaatggtt
     1441 ttttccgccg agtaaatccg aacggatact ccatccacaa cctgggtttc aattcctgcc
     1501 tcgtacgcca ggggtgaaac atgaaaggca cggatcggag gataggtaag acgcggtgtc
     1561 ttacttcctt tcggaagcat aatatccacc caatgtggaa tccgggtggt gagattatga
     1621 aacgccagtg ctgacaccag gccgataatc cctttgggaa ctcgtgttgc aaccgttacc
     1681 aaatctggat ggtcgggcca gggcaagtcc gccaatcggt aaacgccccg gctcagttcg
     1741 atcagttttc cttcatcacg caagtgatag agtgtgcgcg gatgaatccc aagggcaagc
     1801 gcttgcgaag ctcgaactgt ccccccagcc tgttgaataa agtcgagtgc ttgagtcaca
     1861 gggaatgcct ttcttcttta tggatag
//
LOCUS       DAOQDO010001387         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62600_length_1887_cov_2.517467, whole genome shotgun sequence.
ACCESSION   DAOQDO010001387 DAOQDO010000000
VERSION     DAOQDO010001387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62600_length_1887_cov_2.517467"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKZ53_19115"
     CDS             <1..325
                     /locus_tag="PKZ53_19115"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ42606.1"
                     /translation="LPVKSGGRVHFLKVTEIEWIEASDQYVLLHVAGKQHLLRDTLNR
                     LETQLDPDRFFRVHRSALVNLDFVCELELNENGDGTIALSNRTSIKLSRSRREKFEEA
                     LVRRS"
     gene            646..>1887
                     /locus_tag="PKZ53_19120"
     CDS             646..>1887
                     /locus_tag="PKZ53_19120"
                     /inference="COORDINATES: protein motif:HMM:NF015081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42607.1"
                     /translation="MTTSRRVAGMVFLPIFFVLFTASALGQAPTLDPKWSDAPSPRII
                     RAIIEDEIIHLGIQDKVTAEDLNVLTRLKVKADSLMASDEERTRAYKELLTKLAQLTG
                     APAGVETRLDRTVQAAVGLSKGSVVLPGEFPQVEKRGSGPVPVILIPDIGLDWTVYES
                     LMNRNLKAYTMYALTLPGSAGTSPYPRPRIRNFTNTDWWNAVEQGIVNLIKKENLSDV
                     VLVGQQAGAYVAMKTALDHPDQIKAVVNLNGLLYSPMNSPADKTKAATYQERVQIINS
                     SPSLALWPNSSYSQSLQFASGFSTFYCQNEARQKLLSEMMAKTRLDVMYRYFYELMTT
                     DLSEAAQKLTVPLLVVISSPDAKMTVPGGWNANRMQWEKFKAENPKLPLTLVTFEDTR
                     AYATEDSPEDLDRAIGEFLAGK"
BASE COUNT          475 a          502 c          451 g          459 t
ORIGIN      
        1 ccttccagtc aagagtggcg gacgagttca ttttctgaaa gtgactgaga ttgaatggat
       61 tgaagcctca gatcaatatg tcctgctgca cgtcgccggc aaacagcatt tgcttcgtga
      121 cactttgaat cggctcgaaa cacaacttga tccggaccgg ttttttcgcg tccatcggtc
      181 agcgcttgtc aatttggatt ttgtctgtga actcgaactc aatgaaaacg gagatggaac
      241 gattgccctc agcaatcgca ccagcattaa actcagccga agccgtcggg aaaagtttga
      301 agaagccctg gtgcggcggt cttgaaggca catctttgga gtgcggtgac ttgtcaccgc
      361 tttccactcc aaaatttctt tctggcttac ccttgatttc cctcaactca caacacgcaa
      421 ccagcaattc gctccatcgg tgcaaccttc ctgaccggac agccgtgttg acgctggaac
      481 ccaggtcgga gtcatccagc ggcctgatac cattttggaa tgaaatcctc gcattatgga
      541 aaggctaaaa tgttgaaaat attgtatttt cttcagccct tagcccgata ccctcagccc
      601 aaattggtat gatccatccc cactacaatt caatggagca ttgctatgac caccagtcgc
      661 cgagttgcag gaatggtctt tctgccgatt ttctttgtgc tgtttaccgc cagtgccctg
      721 ggacaagccc cgacgctgga tcccaaatgg agcgacgctc catcacctcg aatcattcgg
      781 gccatcatcg aagacgaaat aatccatttg ggcattcagg acaaagtcac ggcagaagac
      841 ctgaatgttt taaccagact caaagtcaaa gcggactcac ttatggccag cgatgaagaa
      901 cgcacccgtg cctacaaaga attattgacc aaattggccc aattaaccgg cgcgccagcc
      961 ggagttgaaa cccgccttga tcgcaccgtg caggcagcag ttggcctttc aaaaggcagt
     1021 gttgtcttgc cgggcgaatt tccccaggtt gaaaaacgcg gctccggtcc ggtcccggtt
     1081 attttgattc ccgatatcgg gctggattgg acggtgtatg aatcgttgat gaaccgcaat
     1141 ttaaaagcct acacgatgta tgctttaacg cttccaggtt ctgccggtac gtcgccctat
     1201 ccacgtccca ggattcggaa tttcaccaat acggattggt ggaacgctgt cgagcagggc
     1261 attgtcaacc tgatcaaaaa ggaaaacttg agcgatgtgg ttctggttgg ccagcaagct
     1321 ggtgcctatg tcgcgatgaa aaccgccctt gatcatccag accagatcaa ggccgttgtg
     1381 aacctcaatg gattgttgta tagcccaatg aattccccag ccgataaaac caaagcggca
     1441 acttaccagg agcgagttca aatcattaac agttcgccct cgctggcgct gtggcccaac
     1501 agttcctata gccagtcact ccagtttgcg tctggttttt caacctttta ctgccagaac
     1561 gaagcccggc agaaactttt gtctgaaatg atggccaaaa cccggcttga tgtgatgtac
     1621 cgatactttt acgaactgat gacgaccgac ctgtctgaag cagcccaaaa gcttaccgtc
     1681 ccgttgctgg tcgtcatctc ttctccagat gccaaaatga cggttccagg tggatggaat
     1741 gccaatcgta tgcagtggga aaaattcaaa gcggaaaacc ccaaacttcc gctcaccctg
     1801 gttacttttg aagatacccg tgcctacgcc accgaagaca gcccggagga tttggatcgg
     1861 gcgattggcg agtttctggc tggaaaa
//
LOCUS       DAOQDO010001388         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62662_length_1886_cov_2.716002, whole genome shotgun sequence.
ACCESSION   DAOQDO010001388 DAOQDO010000000
VERSION     DAOQDO010001388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62662_length_1886_cov_2.716002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKZ53_19125"
     CDS             complement(<1..181)
                     /locus_tag="PKZ53_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ42608.1"
                     /translation="MSRVVSPHQIAASLTELWSPRIIAEVDDTYIKVAKVQGTFTWHA
                     HADEDGRRLAQRLAGR"
     gene            complement(258..869)
                     /locus_tag="PKZ53_19130"
     CDS             complement(258..869)
                     /locus_tag="PKZ53_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42609.1"
                     /translation="MKKYLCLILILFLGHMASAQTPTPPLEISLKNKDQREEQTKHQL
                     QRLLSTYDLSKWIFTRKILIESGFGVIPHSHPVLTLSTRHLKDDELLLATFIHEQIHW
                     YLEQNPKETEAAIKELNVMFPKVPVGFPDGAKDEASTYLHLLVCYLEYQAVREFLGEL
                     RAKQVIQFWTTDHYNWVYKTVLERDRDLGSLIRKQKLLPLKQN"
     gene            complement(960..1400)
                     /locus_tag="PKZ53_19135"
     CDS             complement(960..1400)
                     /locus_tag="PKZ53_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42610.1"
                     /translation="MLIAFWIVLGLNALWFGAAFRYFSLTPETAAKLLVPKSARNSPL
                     FETITASVRFLGGMNLALSVFAVLLLINRQLFPETHQNALFAFVFAVAHASQFAYNLP
                     VALGGGRKGEALWPVLSGPMLLIFVVDGVLMLANTLVGIWLLGQ"
     gene            complement(1504..>1886)
                     /locus_tag="PKZ53_19140"
     CDS             complement(1504..>1886)
                     /locus_tag="PKZ53_19140"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNJ42611.1"
                     /translation="ILPVHESLHAAAYRWIALADSVHIRYDFRYLTAACLADRVVVSQ
                     RPFAWVCLAPSLVINPVLLGIGLILPAGIWQLLVVSALLLHIAAASGDIALVNLLWHE
                     RASGGLITYDDLSAGESYFFRAQV"
BASE COUNT          512 a          477 c          485 g          412 t
ORIGIN      
        1 atcggccagc cagcctttga gcgagacgcc gcccgtcttc gtcagcgtga gcgtgccagg
       61 taaaggtgcc ctggactttg gcaactttga tgtacgtgtc atcaacttcg gcgatgatgc
      121 gcggggacca caattccgtc agtgaagcag cgatttggtg aggcgaaacg acgcgtgaca
      181 tagaggctcc tgaaaattcc aaaatgggaa accagcatag caaggagtct gtgatcggaa
      241 aaccgttcct ggccaggtta gttttgcttg agcggtaaga gcttttgctt gcggattaaa
      301 cttcccagat cacggtcgcg ctccaacacg gttttataca cccagttgta gtgatccgtt
      361 gtccaaaatt ggatgacctg tttggcccgg agttcaccga ggaattccct gactgcctga
      421 tattccaggt aacagacaag caaatgaaga taggtcgatg cttcatcctt ggcgccatct
      481 ggaaaaccaa ccggaacttt gggaaacatg acgttgagtt ccttgatggc cgcctcagtt
      541 tcctttggat tctgttccag gtaccagtga atctgctcat ggataaacgt cgccagcaac
      601 aactcatcat ccttcaggtg ccgggtgctc aatgtgagaa ccgggtggct atgtggaata
      661 acgccaaatc cgctttcaat caggatcttt cgggtaaata tccactttga gagatcatag
      721 gtggaaagaa gtcgctgcaa ctggtgtttg gtttgttcct cccgctggtc tttgtttttg
      781 agactgattt caagtggcgg ggttggggtt tgtgcggagg ccatgtgtcc gaggaaaaga
      841 atcaaaatca ggcacagata ttttttcata gacattgaaa atccccagat agttgaatca
      901 cgaaataccc gaatagaatc aatcatgaac cagatctgat atgtgtaaag gagcgctggt
      961 tactgaccca gcaaccagat gcccaccagc gtattggcca gcatcaacac cccgtcaacc
     1021 acaaagatta atagcatcgg cccactcaaa accggccaca gggcttcacc tttgcgacca
     1081 ccgccaaggg caaccggaag gttataggca aactggctgg catgggccac ggcaaacaca
     1141 aaggcaaaca gggcattttg gtgtgtttcg ggaaacaact gacgattgat caataaaaga
     1201 accgcaaaaa ctgaaagcgc caggttcatc ccgcccagaa agcgcaccga agccgtgatc
     1261 gtttcaaaca gcggagaatt tcgggctgac tttggcacca gcaattttgc ggctgtctca
     1321 ggtgtgaggc taaagtacct gaacgccgca ccaaaccata gtgcattgag tccaagcacg
     1381 atccagaagg caataagcat tgagggaact ccagcaattt caaggtttta ccagatgatt
     1441 aattgatttg tccggtgaaa gagtggctct ctccctgtat ctttgtcatc aggcccggtt
     1501 tgatcatact tgtgcccgga aaaagtagct ctccccagcc gagagatcat cataggttat
     1561 caatccacca gacgctcgtt cgtgccagag gagattcacc agggcgatgt ccccgctagc
     1621 ggcggcaata tggagcaaca aggccgaaac aaccagaagt tgccagattc cagccggtaa
     1681 gatcaaccca atccccaaca gcacagggtt gatcaccagt gacggtgcca ggcaaaccca
     1741 ggcaaatggg cgctggctca caacgacgcg atcagccaga caggcggctg tcagatatcg
     1801 gaagtcatac cggatgtgga ccgaatcggc caaagcaatc caccggtaag ccgccgcatg
     1861 caacgattca tgcaccggca aaatga
//
LOCUS       DAOQDO010001389         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62716_length_1885_cov_3.350820, whole genome shotgun sequence.
ACCESSION   DAOQDO010001389 DAOQDO010000000
VERSION     DAOQDO010001389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62716_length_1885_cov_3.350820"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKZ53_19145"
     CDS             <1..246
                     /locus_tag="PKZ53_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42612.1"
                     /translation="HVEEQLKSIENFFSLVNDQVVTMNTMESITTLDSTFETLLANIE
                     TTKQILEETNPIMTTLNTIENINNSPARPQPQMRVHQ"
     gene            708..>1885
                     /locus_tag="PKZ53_19150"
     CDS             708..>1885
                     /locus_tag="PKZ53_19150"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNJ42613.1"
                     /translation="MSTGELAQDCLPDVLRRLYVNRESGELKLSYRGRQKAIYFELGA
                     IIFASSDAKEDRIGETMMRHGTLSQADFERASAIMRPGKRFGQTLVELGILSDRDLLM
                     NVTFQILDIVYSVFEWPAGTYEFTPQDNVVPADLRLDLSTASIILEGVRRIKDFQLIQ
                     RGLGDLNRLIGPSTSPLLRLQTLSLKPIERQIIDAVKEPMNVLQLLLVVGAPPDVVLR
                     GTYGLLSAGVLERLGAPKINKKTGRISIPKSLYAQAAQAQPVVTEAEVPNTSNDPALM
                     ELIGKLRDRLMTNDPYIILGVTWHSTQDEIRDAYYRLAKDLHPDRFQGAAREVRQDVE
                     FLFTRITSCYEMLKTPTSSKSAPLPPPPPLPQYSAPLSPPQVSAPLPQFLVPPSAQQS
                     S"
BASE COUNT          451 a          488 c          452 g          494 t
ORIGIN      
        1 catgttgaag agcaattaaa gtcaatcgaa aacttcttct ctctggtcaa tgatcaggtg
       61 gtcaccatga acaccatgga aagcatcacc acgctggatt cgacctttga aaccctgctg
      121 gccaatattg aaacgaccaa gcagattctt gaagaaacca atccgattat gaccacgctc
      181 aatacgattg aaaacatcaa taacagcccg gcgcgaccac aaccccagat gcgggttcat
      241 caataagccc ggtctccaac ttgattttcc tgaaacagcc gctggaacgt gtttccacgc
      301 ggctgtttcg gcttttaaat cgaaggagtt accatctggg gaaatgaatt ttgaagggag
      361 ggactcctgg tgaaaaaaga cagcagttgc gccttttttc cctgccgagt tcctgctatc
      421 tttctcgttg gactctcttg cagatcactt caattgcctc agtcgtcact tttcaaaata
      481 aacccatcgt gccgttccag tgtctgattc ctgctcagtt gctttcacag gcagcggatg
      541 gggcaccaat tgtggtcgcg cctttcatct taggttcatt tgatttctgg acacatagct
      601 ttgacccgca ctgaggtcaa ggtgtatctg acttgcagcg ggggcaggtc aattgcttgc
      661 aaactactga gtgcaaagac ttttagagtg gagaggaata tacccaaatg agtactggtg
      721 aattagcaca agattgcctc ccggatgtgc ttcgccggct atatgtcaac cgcgagagtg
      781 gggaactgaa actcagctat cgcgggcgac aaaaggctat ttattttgag cttggcgcaa
      841 ttatttttgc ttccagcgat gcgaaggaag atcgtattgg tgagaccatg atgcgccatg
      901 gaacactctc acaagctgat tttgagcggg cttcagccat catgcgccct ggaaaacgct
      961 ttgggcaaac tctggttgag ctgggcattc tctcggatcg tgacttgctg atgaatgtca
     1021 catttcaaat cctggatatt gtgtattctg tgtttgaatg gccggctggc acctatgaat
     1081 ttaccccaca ggataatgtc gttccggctg atttgcggct tgatctctca accgccagca
     1141 ttattttaga aggagttcgg cggattaaag actttcagtt gattcagcgc ggacttggcg
     1201 atttaaaccg gttgatcggg ccaagcacca gccccttgtt acgcctgcaa accctttcac
     1261 tcaaaccaat tgagcgtcaa atcattgatg ccgtcaaaga gcccatgaac gtgctgcagc
     1321 tcttactggt ggttggggca cctcccgatg ttgtcctgcg cggtacctat ggactgcttt
     1381 cggctggtgt tttagaacgg cttggcgcgc caaaaatcaa taaaaaaacc ggtcggattt
     1441 caattccgaa atcactttat gctcaggcgg ctcaggcgca gcccgtcgtt accgaagctg
     1501 aagttcccaa taccagcaat gatccggcgc tcatggaatt gattgggaaa ttgcgagatc
     1561 gtctgatgac caatgatccc tatatcattt tgggagtcac ctggcattca acccaggatg
     1621 aaatccggga tgcctactat cgtctggcca aggacctgca tcctgatcgg tttcaggggg
     1681 cagcgcgtga ggttcgtcag gacgtagaat tcctgtttac ccggatcacc agttgttacg
     1741 aaatgctcaa gacgccgact tcttccaaat cggcgccact cccacctccg cctccgctgc
     1801 ctcagtactc agcaccgctt tcaccacccc aggtttcagc tccgttgcct cagtttttag
     1861 taccgccgtc tgcccagcaa tcgtc
//
LOCUS       DAOQDO010001390         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62793_length_1884_cov_2.934937, whole genome shotgun sequence.
ACCESSION   DAOQDO010001390 DAOQDO010000000
VERSION     DAOQDO010001390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62793_length_1884_cov_2.934937"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="PKZ53_19155"
     CDS             <1..1768
                     /locus_tag="PKZ53_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNJ42614.1"
                     /translation="HTGRMMTGRRWSDGLHQAVEAKEGVKIEKETQTLATVTLQNYFR
                     MYDKLAGMTGTAETEAPEFAKIYNLDVTIIPTNRAMIRVDNPDLVYRTETEKWNASVE
                     EIKELYAKGQPVLVGTASVENSEHLSALLKKINIPHNVLNAKYHEREAEIVAQAGRKG
                     MVTIATNMAGRGTDILLGGNPEFLAKEILKRQEINPDEATPEQLEPALLEAQAIVAKE
                     HEEVVSLGGLHILGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFGGER
                     VKNLMLRLGMEEGVAIESKMVSKQVERAQKSVEGHNFSIRKHLLEYDDVMNKQRETIY
                     GLRRELISEGGEDRREFINVAEDLLTDIVEEFASEDVHPDEWNIDGLKIKLQDIYGFD
                     LEAHELDFDALSASELRDEIWKLLKDVYAEKEAAVGSDRMRELERYIMLNVVDAQWKD
                     HLAQLDHLKEGISLRAYGQKDPLVEYKKESRVLFDEMIDRIDEESVRFMFNMRIEVAE
                     QAAENIEAPEEEAPAPTPVPMRRPVRRQPQMVYTKANAGAARAGETAEAKAQPVVKLP
                     KVGRNEPCPCGSGKKYKQCHGA"
BASE COUNT          524 a          454 c          521 g          385 t
ORIGIN      
        1 acataccggg cgcatgatga ctggtcgtcg ctggtcagat ggattgcacc aggccgttga
       61 agccaaagaa ggggtgaaga ttgaaaaaga aacccagacc ctggcgacgg tcacgctgca
      121 aaactatttc cgcatgtatg acaaacttgc tggaatgacc ggtaccgccg aaacagaagc
      181 gcctgaattt gcaaagatct acaaccttga tgtgaccatt attccaacca atcgggcgat
      241 gatccgcgtg gataatccag acctggtgta tcgcactgag actgaaaaat ggaatgcctc
      301 cgtggaagaa atcaaggagt tgtatgccaa gggacagccg gttctggtcg gaacggcatc
      361 agtggaaaat tcagaacacc tgtcagccct gctcaaaaaa atcaacatcc cccacaacgt
      421 gctcaacgcc aaataccacg agcgcgaagc cgaaatcgtg gctcaggctg gccggaaggg
      481 catggtcacg attgccacca acatggctgg tcgtggaacg gacattttgc tgggcgggaa
      541 tccagagttt ctggccaaag aaatcctgaa acgccaggaa atcaaccctg atgaagccac
      601 acctgaacag cttgaacccg cacttcttga agctcaagcc attgttgcca aagaacatga
      661 ggaagttgtg agcctcggtg gcctccatat ccttggtact gaacgccacg aatcccgccg
      721 gattgacaac cagctccgtg gtcgtgccgg acgtcagggc gaccccggtt catcccgttt
      781 ttacctctcg ctcgaagatg atctgatgcg catcttcggt ggcgaacggg ttaaaaacct
      841 gatgcttcgt ctgggaatgg aagaaggcgt ggcgattgaa agcaaaatgg tttcgaaaca
      901 ggtcgaacgc gcccagaaat cagttgaagg ccataacttc tcaatccgca agcacttgct
      961 tgaatatgac gatgtcatga acaagcaacg tgaaacaatt tatggtttgc ggcgcgaatt
     1021 gatctcagaa gggggcgaag accgacgcga atttatcaac gtggcggaag acctgctcac
     1081 cgacatcgtt gaagagtttg ccagtgagga tgtccatcca gatgagtgga atattgatgg
     1141 tctcaaaatc aaactccagg atatctatgg gtttgacctt gaagcccatg agctggattt
     1201 tgatgcgctt tcagcctctg aattgcggga tgaaatctgg aaattgctga aggacgtgta
     1261 tgccgagaaa gaagccgccg ttggttcaga ccgcatgcgg gaacttgagc gctacattat
     1321 gctcaatgtg gttgacgccc agtggaaaga ccatctggcc cagcttgacc atctgaaaga
     1381 aggcatcagc ctgcgcgctt atggtcaaaa agatccgctg gtggaatata agaaagaatc
     1441 acgggtgctc tttgacgaaa tgattgaccg gattgatgaa gaatcagtgc ggtttatgtt
     1501 caatatgcgc attgaggttg ccgaacaggc ggctgaaaac attgaggctc cagaggaaga
     1561 agcaccagct ccaacaccgg tgccgatgcg acgacccgtc cgccgacaac cacaaatggt
     1621 ctataccaaa gccaatgctg gtgccgcccg agctggcgaa acagcagaag cgaaagctca
     1681 gcccgtggtc aagctgccaa aggttggtcg caacgaacct tgcccctgcg gttcaggcaa
     1741 gaaatacaaa cagtgtcatg gggcgtaaag cctgggctga agaagtcggg ctgaagaagt
     1801 cgggctgaag acatcgggct gaagacgtcg ggctgaagat atcgggctga agacgtcggg
     1861 ttgaagaact ggttttattt catc
//
LOCUS       DAOQDO010001391         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62863_length_1883_cov_2.830416, whole genome shotgun sequence.
ACCESSION   DAOQDO010001391 DAOQDO010000000
VERSION     DAOQDO010001391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62863_length_1883_cov_2.830416"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKZ53_19160"
     CDS             <1..322
                     /locus_tag="PKZ53_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42615.1"
                     /translation="VSSDTVKPTVTVTSPSASTKKVKRGDPINVAWTSTDNGTLAKHD
                     IQLSVDDGATFGTSLATGLAGTARSFTATAPSSKVKKAIIKVIATDAAGNTGEGLSTK
                     FKVK"
     gene            820..>1883
                     /locus_tag="PKZ53_19165"
     CDS             820..>1883
                     /locus_tag="PKZ53_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42616.1"
                     /translation="MFNLIALVKKRGPINTGPSIHLMLSRWVGGGLLALILLVLMTPH
                     SSGNQLTQKPHRLELPFNARFKSPVMQPQVISRAHVQHRTRSPQHINLAPQAIQPGTA
                     LEKVTFYGNGITSIIAAADTNNDKIPDGTARETFTAGNPELEEFTALAVSARTQKVYA
                     GSLLFEGTNANEGVLTVLENPTGNFKGTIVRKIPLGKGMPVGIVVKNSGDGDIVIVMS
                     VYYQASPFILTDGDFVSITAYLPGPDGIPDGSRKVDVLSAGQLLLGLDQVAFSFGGMA
                     LSQNGNLIANLVAKNAIDGFAGGALFVFTDNDGDGIPDNLMAPTRFAEFSDQNVANLI
                     AATSVVPLAGGKIGVLGADI"
BASE COUNT          464 a          480 c          470 g          469 t
ORIGIN      
        1 cgtcagttcc gacacggtga aaccgaccgt gaccgtgact tcgcccagcg ccagcaccaa
       61 gaaggtcaaa cgcggcgatc caatcaacgt agcgtggacc tcaaccgata acgggacgct
      121 tgccaagcat gacattcagt tgtcagttga cgatggtgcg acatttggaa cctcactcgc
      181 aaccggattg gctggaactg cccggtcgtt tacggccacc gccccaagct cgaaggtcaa
      241 gaaggcgatc atcaaggtga ttgccaccga cgcggcgggc aatactggcg aaggtctctc
      301 aaccaaattt aaggtcaagt aaatttcagg gatgacccaa aatgaactgg aagggaactg
      361 tttcaactcc aggttattgt gcatccctca gctcaaatgg ggttgcgagt agaatctact
      421 cgcaacctct ttgctttttt tgttactctc tcaaaacgtc ctcacttaat aacaactgaa
      481 ccatctggtt ccaacgacaa gcggtggttt ctcaacaggc ttcctgcctg aacggatttg
      541 gttcttcctc accacctgaa atcagcccgg tgcattttcc tcctgtgcgc tgagtccaaa
      601 tccaaagtgg atatctttct gaccgttgtt tttggtgccc aaccatcata cacattttgg
      661 attctttatt tctggaagtg tttctcctgg caggggttct gtcattcact actcaccaga
      721 tcaaataccc tgtattgaga aacagtcaaa tcattcaatc aaatgagctt agtaaactac
      781 tgactactga ccactgacta caaactggta ttcttcatga tgtttaatct gatagccctt
      841 gtaaaaaagc gcggaccaat caacacaggc ccctcgatcc acctgatgct gagccggtgg
      901 gttggtggag gactgctggc cctgatcctg ctggtattga tgacaccgca ttcctcgggc
      961 aaccagttga ctcaaaaacc acaccggctc gaattgccct tcaatgccag attcaaatcc
     1021 ccagtgatgc aaccccaggt gatttcaagg gcgcacgttc agcaccggac caggtcgccc
     1081 caacacatca atctggcacc tcaggcaatt caacctggca cggccctgga gaaggtgacg
     1141 ttttatggga atggcattac ttcgattatc gccgcggctg acaccaataa cgataagatt
     1201 ccagatggga ctgctcgtga gacatttacc gctggtaacc ctgagcttga agagtttacc
     1261 gcgctggccg taagtgctcg gacccagaaa gtctatgccg ggtcactcct gtttgaaggc
     1321 accaatgcca acgagggggt tttaactgtg cttgagaatc caactggaaa tttcaaaggc
     1381 acgattgttc gaaaaatccc acttggcaaa gggatgccag tgggaattgt ggtcaaaaac
     1441 tcaggggacg gagacatcgt gattgtgatg tcggtctact accaggcgtc accgtttatt
     1501 cttacggacg gcgattttgt ttcaatcacg gcgtatctgc ccggtccgga tggaataccg
     1561 gatggatccc gaaaagtgga tgttctttct gccggacaat tactacttgg tcttgatcag
     1621 gtagccttta gctttggggg aatggcactc agtcaaaatg ggaacctgat tgccaatctg
     1681 gtcgcgaaaa atgcgataga tggatttgcc gggggggccc tctttgtttt taccgacaac
     1741 gatggggatg gaattcctga taacctgatg gctccgaccc ggtttgctga gtttagtgat
     1801 cagaatgtag cgaatttgat tgccgcgacc agcgtggtgc cgctggctgg ggggaaaatc
     1861 ggtgtgctcg gtgccgatat ctt
//
LOCUS       DAOQDO010001392         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62885_length_1883_cov_2.217724, whole genome shotgun sequence.
ACCESSION   DAOQDO010001392 DAOQDO010000000
VERSION     DAOQDO010001392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62885_length_1883_cov_2.217724"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..931)
                     /locus_tag="PKZ53_19170"
     CDS             complement(77..931)
                     /locus_tag="PKZ53_19170"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ42617.1"
                     /translation="MSFSGFFDWRSLRRNLLPFHPLVWLGLTVCCLISFSLPALATPP
                     DGKVVGQIKDSTGDPIPGATIELKPSLAGSFFSLVTDANGVFSVEDLHPGRYLVKISQ
                     PGFSTRTTEVEVQPGDATRFEIVLTPGLITETVTVTAARTELVAQDTPVPVSVIDRTQ
                     LERQPVATIGDLFRSLPGVSTASEGPFQVRPRIRGLESNRVLILVDGERLNHTRTSTS
                     ESGIEIGLVGVDQIETVEVLRRSGSVLYGTDALGGTINLITRDTLPCQTSGFRFSGGI
                     RAWRARDY"
     gene            complement(1558..>1883)
                     /locus_tag="PKZ53_19175"
     CDS             complement(1558..>1883)
                     /locus_tag="PKZ53_19175"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNJ42618.1"
                     /translation="FAGRKKNIIVLSTGDNVSPLEVEQAILSHPGVSNCIVVAAQARD
                     TSEVPWTFITRLDETLTELALETFLKERISAYKIPQKIIFVPEIPVGLTGKIRHPETP
                     FDLIA"
BASE COUNT          476 a          449 c          516 g          442 t
ORIGIN      
        1 ttttccgtgt tttccgtggt taaaatgtct ggaaattttc ggtaaggtac ttaaaaacga
       61 cctccattgg atatagtcag tagtccctag cccgccaagc tctgatgcca cccgaaaacc
      121 gaaacccgga tgtctggcac ggcaaggtat cgcgggtgat gaggttgatg gtgcccccaa
      181 gggcgtctgt accataaagg acagaaccgc ttctgcgcag aacttcaact gtttcaatct
      241 gatccacacc gaccaggcca atttcaatgc ctgattcgga ggttgaggtt cgcgtatggt
      301 tgaggcgttc gccatcaacc agaatcaaca cccggttgct ttcaaggccg cgaatccggg
      361 gccgaacctg gaaggggcct tcgctggcgg tgctgacacc gggcaaagat cggaacagat
      421 ctccaatcgt agcaaccggc tgtcgctcaa gctgggttcg gtcaatgact gaaaccggaa
      481 ctggcgtatc ctgagcgacg agttcggttc gggccgccgt gacggtgacc gtttcggtga
      541 tcaaccctgg cgtgaggaca atttcaaacc gggtcgcatc tccaggctgg acttcgactt
      601 cagtggttcg ggttgagaat ccaggttgtg aaattttgac caggtagcgt cccggatgga
      661 gatcttcgac ggaaaatact ccattggcat cggttacaag tgaaaagaat gagccggcga
      721 gggaaggttt gagttcaatg gtggcacccg gaatgggatc cccagtgcta tccttgatct
      781 ggccgaccac ttttccatcg ggaggcgtcg ccagagccgg cagtgaaaat gaaatcaggc
      841 aacagaccgt gagtccgagc caaaccagcg ggtgaaaagg caacaaattg cgacgcaggc
      901 tgcgccaatc aaaaaagcca gaaaacgaca tgtggagcac ctcagaccgg acgcaaaata
      961 aaatctggtg tgtgtgtttt gtcgcagtgg cgtgctatga tcggcggcgc cactgggagc
     1021 ggatgcgcaa acagaagctg cagtggcgaa aaagggcctc cggtgtggca gcaccggagg
     1081 cttttcgctt catgctatgg agaacaacca acgaaggaac aaccgcaccg tgaagccaat
     1141 gcccggttca cgatgcttga tcaaccccac tcatctggca ggatgagtgg ggttgatctt
     1201 gctaaaattg aaattcattt tcaattaaca taagcaaact atgactggaa ccggtttttg
     1261 tcaagcattt tttcaaagtg cgactatttt ttgcagccac gttcggaaaa tggcagaggt
     1321 gtccgcattc ttaatacgat agttcttcag acaataattt ctgcctggga gcgcgggcgt
     1381 ctcacccgcg ctcccggaaa tagttatctg aaagactatc caacgagagt tgaataattc
     1441 atgaactcaa gtgttgtgtt ttgagatgac acaggccaaa agtcaccaga ctggggtcgg
     1501 ttgaaatttg gcaaagagtg atggcaatac tgtttttcaa ccctttcgtc ttttggctca
     1561 ggcgataaga tcaaatgggg tctctggatg tcgtatcttg ccggttagtc caaccggaat
     1621 ttctggaacg aagatgattt tctgtggaat tttgtaggca ctgattcgtt ctttcaaaaa
     1681 agtctcaagt gccagttcgg ttaaggtttc gtccaaacgg gttataaatg tccagggcac
     1741 ctctgaagtg tcacgggcct gggcagcgac cacaatacaa ttggaaactc ctgggtggct
     1801 caggatggcc tgctcgacct caagcggaga aacattgtcg ccggtactca ggacaatgat
     1861 gtttttcttg cgcccggcaa acc
//
LOCUS       DAOQDO010001393         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62972_length_1881_cov_3.454545, whole genome shotgun sequence.
ACCESSION   DAOQDO010001393 DAOQDO010000000
VERSION     DAOQDO010001393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62972_length_1881_cov_3.454545"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1344
                     /locus_tag="PKZ53_19180"
     CDS             316..1344
                     /locus_tag="PKZ53_19180"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNJ42619.1"
                     /translation="MNQISLLKQQLDIVQIISGFIPLKPKGTVWVGLCPFHQEKTPSF
                     TVSKTKQIFKCFGCGEGGDVLTFISKFERLSLCDSIQRLKEIAGAPNEARKNSSKRCD
                     TSSVGLTGGLSLPVSDLAPTPTLRVDLKPVLERYQAALPGSLGEIFLKHRGISLKTAQ
                     KFGLGYAADGQWEHRIPTGKLARNWKWGRLVFPHTDPAGNLINLYGRAVGLDSKVPRD
                     QRHDHLPGQKGYFNSQTLAHSVSPVWCEGPFDALTLLELGIPAVAIFGIDGWHWEWVH
                     SLQTFYFAFDADAAGQIGWKRLARQCVLRGKKVWFIPPEVYGERKDINAAHCAGTLQI
                     TAFTRVLG"
BASE COUNT          463 a          408 c          461 g          549 t
ORIGIN      
        1 ggttatcgtg gtcacatgtc ggggttatcg tggtcacatg tcggggttat cgtggcagaa
       61 agtgccactt aacttctttg gaatcaacaa tattcctggc ctaataatag taataatatt
      121 aaatagggaa tatgcctgcg acatattcac ctagtacaat gcaccggttt gcccttctga
      181 ggcgtgaccg aaatgcaccc gtttgccttt ctgagaagca atcgcaatgc ttttttgcgg
      241 tttagagaga aattcaactg gtttcgtcgt gcctcaacct ttgattggtt cttccttcaa
      301 ttcctggacg cagccatgaa tcaaatttcc ttgctcaaac aacaacttga tattgttcag
      361 atcatttcag gttttatccc actcaagcca aaagggacgg tttgggttgg cttatgtccc
      421 ttccaccagg aaaaaacccc aagctttacg gtgagtaaga ccaaacaaat cttcaagtgt
      481 tttggatgcg gggaaggtgg cgatgtgttg acattcattt caaaatttga acgactttct
      541 ctttgcgact cgattcagcg gctcaaagag attgccggcg cacccaatga ggcaagaaaa
      601 aacagtagca agcgatgtga taccagttcc gttgggctga cgggaggtct ttccctgcct
      661 gtatccgatc ttgccccaac gccaacactg agggttgatt tgaaaccggt gctggaacgg
      721 taccaggcgg ccctgccagg aagccttggc gaaatttttc tcaagcatcg cggaatttca
      781 ttgaaaactg cgcaaaagtt cgggttggga tacgcagcgg acgggcaatg ggaacatcga
      841 ataccgactg gcaaactggc tcgaaactgg aaatggggaa ggctggtgtt tccacacacc
      901 gatccggctg gcaatttgat caatctgtat ggccgggcag ttgggcttga ttccaaagtc
      961 ccgcgtgacc agcggcatga tcatttacct ggccagaaag ggtattttaa ctcacaaaca
     1021 ctggctcatt ccgtatcacc tgtctggtgt gaaggaccct ttgatgcact gacgctcctt
     1081 gagttgggaa ttccggcggt tgcgattttt ggaattgatg gatggcactg ggaatgggtc
     1141 cattcccttc aaacctttta ctttgctttt gacgccgatg ccgcaggaca gatcgggtgg
     1201 aaacgactgg cgcgccaatg tgtgttgcgg ggcaaaaagg tgtggtttat ccctccggaa
     1261 gtctatggcg aacgcaaaga tattaacgcg gctcattgtg cagggacact acaaatcact
     1321 gcgtttacaa gggttctagg ttaatttatc cctggttttg gtaattcttc ccacaggaac
     1381 tggccagaac tgaacaggtg tcctggattc agaatcgcat gtcaaaattt tagacactgc
     1441 ttgtcatttt catggcaggt tctgtcttgc cagcgagcaa cttgattttc tgaaactgaa
     1501 gcctatctca atttttcacc cggtttagcc taacgattaa agaactcttc tgttttggag
     1561 ttacccaggt aactccattt tttgtctagc tttatatccg gtcaaagacg caagaattca
     1621 gtgaaagtgg tatttttttg ttgggtacag gcaaagcgtt gtttttcaag gtgaaggact
     1681 tattttggtt ttgcgaacga aactggcaca gatcttgtta gaggaatgtc agattcgttt
     1741 atccaaaatc cagttttgct tccatttggt gagaaatgct tttgtcgttg agaaaggttt
     1801 ctttcatctt ttgtgagcgc cgttccttcc atgtgtagaa gttttcatca tgtaactgat
     1861 atgtgaattt tgcctgacac g
//
LOCUS       DAOQDO010001394         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_62991_length_1881_cov_2.716320, whole genome shotgun sequence.
ACCESSION   DAOQDO010001394 DAOQDO010000000
VERSION     DAOQDO010001394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62991_length_1881_cov_2.716320"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="PKZ53_19185"
     CDS             complement(<1..1865)
                     /locus_tag="PKZ53_19185"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ42620.1"
                     /translation="MAVWVLIGLFGIISLISLKLVSVRAQKPEGASQYQALKAQAEKL
                     FAEKSYALAREQYVKVNLAELPPAEARWVQFRLADTLWRSQAATETSDSTQFDQAQHQ
                     LEVLVRDIQRPEDRDLVWAEVQESLGDFWWLRRQSRNWWQGWQYYQQALGYWGNSVNL
                     DQARDRYLAIVFRMTGPPPWADQYYRYGNYGSIPQDIVANARKIAKTPDDRAHTAYLM
                     AASFRYYGGQWEDRERVPSYFEEALKAGKQTEWYDDALFEYAQWMENTGNISWSAETD
                     WKQTPDYLTALDLYRRLTTEFKKGDTRYFDEATEHIKNITRPVIGVSVSNVFVPGSLI
                     DYSLNWRNVKRIDLALYPVDLTQDIRFGQNESSIGNWLQTVNLAGKSTVKTWAKDTGD
                     KGDHRPQYESLRLDDPLPVGAYVLEASAGNLKARELVLVTNAALVVKSAPSKLVALMT
                     DAGSGAPLAGAKLKVFEQYYQNSTWKIREHALTTNENGLAETQTFPAESTQHYFWVSG
                     QHQEHQAICSTDQYTSRYGNQWKVYAFTDRPAYRPGESVQWKFVARSKDDQTWTTPAN
                     QMVEFEITDPRGTKVKEDKVKLNEFGSAWGSLDVTENMPLGEYRIQFWTQGRSQN"
BASE COUNT          411 a          501 c          486 g          483 t
ORIGIN      
        1 atattttgcg accgtccctg agtccagaat tgaatccgat attcgccgag cggcatgttt
       61 tctgtgacat caagcgaccc ccaggcgctg ccaaattcat ttaactttac tttgtcttct
      121 ttgacttttg tgccgcgtgg gtcggttatt tcaaactcaa ccatctggtt ggctggggtc
      181 gtccaggttt gatcgtcctt tgagcgagcc acaaatttcc attgaactga ttcaccagga
      241 cgataagcgg ggcgatctgt aaaagcatag accttccact ggttcccata gcgcgacgtg
      301 tactggtcag tactacaaat ggcctgatgc tcctgatgct gtccactcac ccaaaagtaa
      361 tgctgggttg attcagccgg aaaggtttgt gtctcagcca gaccgttttc gttggtcgtc
      421 agtgcgtgct cgcggatttt ccacgtgctg ttttggtaat actgttcaaa cactttgagt
      481 ttggccccag ccagtggggc accgctcccg gcatcagtca tcaaggccac cagttttgac
      541 ggcgcacttt tcacaaccag tgctgcattg gtcaccagca ccagttcccg ggcttttaaa
      601 ttcccagcac tggcttccag cacataggca ccaaccggca acgggtcatc cagccgaagc
      661 gactcgtact gtggacgatg gtcacctttg tcgccggtat ctttggccca ggtttttacg
      721 gttgatttcc cggccagatt gacagtctgc agccagttgc caatcgagga ctcattttgg
      781 ccaaatcgaa tatcctgagt taaatcaaca ggatagagtg ccaggtcaat ccgtttgacg
      841 ttgcgccagt tgagcgaata gtcaatcaag gaacctggta caaagacatt tgaaacactg
      901 acgccaatca ccggacgggt gatgtttttg atatgttcgg tggcttcgtc aaaatagcga
      961 gtgtcacctt ttttgaattc ggtggtgagg cggcgataga ggtcgagggc tgtgaggtaa
     1021 tctggggttt gtttccagtc agtctcggct gaccaggaaa tgtttccggt gttttccatc
     1081 cactgggcat attcaaacag cgcatcgtca taccattcgg tttgttttcc agccttgagt
     1141 gcctcttcaa agtaggacgg cacccgttcg cgatcttccc actggccgcc gtagtagcga
     1201 aatgaagcgg ccatcaaata agccgtatgc gcccgatcat ctggagtttt ggcaattttg
     1261 cgggcattgg cgacgatgtc ttgcgggatc gaaccatagt tcccatagcg gtaatactgg
     1321 tctgcccacg gcggaggacc agtcatccga aagacaattg ccagatagcg gtcgcgagcc
     1381 tggtccaggt tgaccgaatt gccccagtac cccagcgcct gctggtaata ttgccagccc
     1441 tgccaccagt tgcgcgattg tcgccggagc caccagaagt cgcccaagga ttcctgaact
     1501 tcggcccaga ccagatcgcg gtcttccgga cgctggatat cacgcaccag aacttcaagt
     1561 tgatgctgag cctgatcaaa ttgcgttgaa tcagaggttt ccgttgcggc ctgggatcgc
     1621 cagagcgtgt cggccaatcg aaactgtacc cagcgcgcct cagccggagg cagctctgcc
     1681 aggttcacct ttacatattg ctcacgagcc agcgcgtagg atttttcagc aaagagtttt
     1741 tcagcctgag ctttgagagc ctggtattga gacgcgcctt cgggtttttg tgcccgaacc
     1801 gaaaccagct tcaatgaaat gaggctgatg atgccaaaca gcccgatcaa aacccacact
     1861 gccatttttg tgtgcttcat g
//
LOCUS       DAOQDO010001395         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63035_length_1880_cov_4.472877, whole genome shotgun sequence.
ACCESSION   DAOQDO010001395 DAOQDO010000000
VERSION     DAOQDO010001395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63035_length_1880_cov_4.472877"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1168
                     /locus_tag="PKZ53_19190"
     CDS             170..1168
                     /locus_tag="PKZ53_19190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNJ42621.1"
                     /translation="MLLDPQLNLTLRPMQYPVFYEMYRQAIRNTWTVDEVDFSTDLAD
                     LRSRMTPAERHLINRLVAFFATGDSIVANNLALNLYKHINSPEARMYLSRQLYEEALH
                     VQFYLTLLDTYIPDHDERAQAFKAIENIPSIREKAQFCFRWMDSIQELDQLTTVEERR
                     QFLLNLICFATCIEGLFFFAAFAYVYFLRSKGLLHGLAAGTNWVFRDESAHMMFAFEV
                     IKTVRQEEPELFDEELGERIARMIDEAIECETLFAQDLLSQGVAGLSTGDMRQYLEYV
                     ADQRLAMLELPIRYHARNPFPFMDLQDVQELANFFERRVSAYQVGVTGEVAFNEAF"
     gene            1344..1769
                     /locus_tag="PKZ53_19195"
     CDS             1344..1769
                     /locus_tag="PKZ53_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42622.1"
                     /translation="MKLDLEAAQERARKYLEHCEKGYAANLEEANWFQAHSPKSDQQS
                     DRPSLRLIHFVLDETWHLEYEDFFLLTFNCWEETPTGPKETFLGGGGPILVDRATGRV
                     YYPFTVEEDEDIELVFKKRRSIKEGQKDKPFSVGWSNPG"
BASE COUNT          482 a          481 c          451 g          466 t
ORIGIN      
        1 ccagtagaaa agcaatttgt gcttggtgct tcgtgcttgg cccttcgttc ttcgttctta
       61 gaaagaattg gttcctaagc accaggtacc aagtaccaag caccaagcac cggtcaccca
      121 aacagcaatt cttcatcctc tgtttttctc tttcccggag acattcatca tgcttctcga
      181 tccgcaatta aatttgacct tgcgtccaat gcaatacccg gttttttatg aaatgtaccg
      241 tcaggcaatc cgcaacacct ggacggttga cgaagtggat ttctcgaccg atctggctga
      301 tttgcgctcg cgcatgacgc ccgccgaacg ccaccttatc aaccggctgg tcgcgttttt
      361 tgccaccggt gattcgattg tggccaataa tctggcgctc aatctctata aacacatcaa
      421 ttcgccggaa gcccggatgt acctgtcccg tcagttgtat gaagaagccc tgcacgtgca
      481 gttctacctg acgctgctcg acacctacat tccagaccac gacgaacggg cccaggcatt
      541 taaggcgatt gaaaacattc cttcgattcg cgaaaaagcc cagttctgct tccgctggat
      601 ggattcgatt caggaactgg accagctcac caccgtcgaa gagcgccggc aattcctgct
      661 caacctgatt tgctttgcaa cctgcatcga agggctcttt ttcttcgcgg catttgccta
      721 tgtttatttc ttgcgttcaa aggggttatt gcacggattg gctgctggca ctaactgggt
      781 atttcgtgac gagagcgcgc atatgatgtt tgccttcgaa gtcatcaaga ctgttcgcca
      841 ggaagaaccc gaactctttg acgaagaact cggcgaacgc atcgccagga tgattgacga
      901 agcgattgag tgcgaaaccc tgtttgccca ggacctgctc agccagggtg ttgccgggtt
      961 gtcaactggt gatatgcgtc agtatctgga atatgtcgcc gaccagcggc tggcgatgct
     1021 ggaattaccg atccgctacc acgcccgcaa cccatttcca tttatggact tgcaggacgt
     1081 tcaagaactg gcgaacttct ttgaacgccg cgtctcggcc tatcaggtcg gcgtcaccgg
     1141 cgaagtggcg tttaacgaag cgttttagtg ttcgtcagta gtgagtagtc cgtagccttg
     1201 aaaaccagag tatccccaca aattgaccaa ctcctcaatc gcgatacttt gtggcaggac
     1261 acagatcatc gaaaggttaa aaaaacagcc gaacagagcc tgaagaagca ttgttcggct
     1321 atttttttat tcggagcaat ttgatgaaac ttgatctgga agcagcacaa gaacgtgcca
     1381 ggaaatatct ggaacactgt gaaaaaggct atgctgccaa tctcgaagag gcaaactggt
     1441 ttcaggccca cagtccaaag tctgatcagc aatctgatcg tccctctctc cgcctaattc
     1501 actttgttct cgatgaaacc tggcatctgg agtatgaaga cttctttctt ttgacattca
     1561 actgctggga agaaacacca accggaccaa aggaaacatt tctgggaggc gggggaccaa
     1621 tcctcgttga tcgagcgact ggccgggtgt actacccgtt tacagtggaa gaggacgaag
     1681 atattgaact ggtgtttaaa aagcgcagat ccatcaaaga gggccagaag gacaaaccct
     1741 tctcagtcgg ttggagcaat ccaggatgaa actcaatttg gaagcagcac aagaacgtgc
     1801 caggaaatat ctggagcagt attcaaaaac ctttcaccaa gagcaagatc atgctttgga
     1861 ttggttcatt gaacaaaaac
//
LOCUS       DAOQDO010001396         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63108_length_1879_cov_3.148575, whole genome shotgun sequence.
ACCESSION   DAOQDO010001396 DAOQDO010000000
VERSION     DAOQDO010001396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63108_length_1879_cov_3.148575"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>1879)
                     /locus_tag="PKZ53_19200"
     CDS             complement(157..>1879)
                     /locus_tag="PKZ53_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42623.1"
                     /translation="EIGTLTTPDRTISSYWTAVDSYRIYSNSRSALERILDAAQGKRP
                     SLKAAAEFRYMRTIFPGTASEEDGFLYLSDAFIRRVVGAEQKIGRIRQLGCSANLHNL
                     EHAYLYQFYDQGKQTPVTVTDLREQKLLDADDLECPSGGTYQLDDQGLARCSVHGSHR
                     NLTPLLEIPITQATPAEAAGYQNFVERYTQYWSQYFDPVGVRFRLRDKIEVETCILPL
                     IENSIYNNVREAAGGKPVKLSIPVTAATISSIGMKLGNDSPLRKEMLRSFLLDMTGPD
                     SEKFLAAVGDTISLNFCDGAPLFTFAMDTTTALIAGSGLRRGDDAFLGLILSSLTLPI
                     YVSMDITDPAQAEPQFDKILAHAEQRFRMQGERWLTLERYQLNPYRDVPIHTAKLSLF
                     VADFRFAWAVVGKQLIISTQLPVLTSLIDTLKDKPDTAFLAQENPTNLLFSLRPSQFQ
                     HIRPTMAAHWQEKMRSTCLLNLSGVRLLNEQFPVTNPDFNQQFLKRMGYIPFCPAGGS
                     YRIEPTSGVAECTVHGTHTHPRQPIEATGKEPFIQFVESLKTIGASLEFTPEGIRTRI
                     VLDRTKK"
BASE COUNT          401 a          492 c          540 g          446 t
ORIGIN      
        1 tcaaagatcc caccctgggc tacgaactgc tacccatttc acaggttaaa cctgacgtct
       61 tgagccctaa accctcagct ctggttttct tcagccccga gcttgcgagt cttcaacccg
      121 tattcttcag cccacttttt tcagcccgat gttttctcac ttcttcgtcc ggtcaagcac
      181 aatacgggtg cggatgcctt caggtgtaaa ttcgagcgag gccccaatcg ttttcaggct
      241 ttcgacaaac tggatgaacg gctcctttcc ggtcgcttcg ataggttgtc gcgggtgggt
      301 gtgcgtccca tgaacggtgc attcggccac gccactggtt ggttcgatcc ggtagcttcc
      361 acccgccgga caaaatggga tgtagcccat ccgtttcaga aattgttggt taaagtcagg
      421 gttggtgacc gggaattgct cgttgagcaa ccgaaccccg ctcaggttga gcagacaggt
      481 actgcgcatt ttctcctgcc agtgagccgc catggttggg cgaatgtgct gaaactggga
      541 aggccggagc gaaaagagca ggttggttgg attctcctga gccagaaagg ccgtgtctgg
      601 tttgtccttt aacgtatcaa tcaggctggt cagaaccggc agttgggtac tgatgatgag
      661 ttgcttgccc accactgccc aggcaaatcg gaagtcggcg acaaagagcg agagctttgc
      721 cgtgtggatc ggaacgtcac gatacgggtt gagttgatag cgttcgagtg tcagccagcg
      781 ttcaccctgc atccggaagc gctgttcggc gtgagccagg attttgtcaa attgcggctc
      841 ggcctgggct gggtccgtga tgtccatcga aacataaatc ggcagggtga gcgatgacaa
      901 aatgagcccg agaaaggcat catcacctcg acgcaggcca ctcccggcaa tcaaggccgt
      961 ggttgtatcc atcgcaaagg taaagagcgg tgctccgtca cagaaattca gactaatcgt
     1021 gtcgccgacg gctgccagaa atttttcgct gtccgggccg gtcatatcca gcagaaatga
     1081 ccgcagcatt tccttgcgga gcgggctgtc gttcccaagt ttcatcccga ttgaggaaat
     1141 agtggcggcg gtgactggaa ttgacagttt gaccggcttg cccccggcgg cttcacgaac
     1201 attgttatag atgctgtttt caatcagcgg cagaatgcag gtttcgacct cgattttgtc
     1261 acgcagtcga aaccgaacac ccaccgggtc aaaatactgg ctccaatact gggtatagcg
     1321 ctcgacaaaa ttctggtagc cggcggcttc ggctggtgtg gcctgggtga ttggaatttc
     1381 aagcagcggg gtcaaattgc ggtgtgaccc gtgaaccgaa cagcgcgcca gcccctgatc
     1441 gtcaagctga taagtgccgc ccgatggaca ttccagatca tcggcatcaa ggagcttttg
     1501 ttcacggagg tccgtcactg tgactggcgt ttgttttccc tggtcataga actggtacag
     1561 gtaagcgtgt tcgaggttgt gcagattggc gctgcaccca agctggcgaa tgcgtcctat
     1621 tttttgctcg gcacccacca cgcggcggat aaaggcatcg gaaagataga gaaacccatc
     1681 ttcctcgctg gcggtgcccg gaaagatggt gcgcatatat cgaaattcgg ctgccgcttt
     1741 gagtgacggt cgtttgccct gcgccgcgtc gaggatgcgt tccagtgccg accgtgagtt
     1801 tgaatagata cggtagctgt caaccgccgt ccagtaggaa gaaatcgtcc ggtctggtgt
     1861 ggtcaacgtg ccaatttca
//
LOCUS       DAOQDO010001397         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63130_length_1879_cov_2.356908, whole genome shotgun sequence.
ACCESSION   DAOQDO010001397 DAOQDO010000000
VERSION     DAOQDO010001397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63130_length_1879_cov_2.356908"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1211
                     /locus_tag="PKZ53_19205"
     CDS             147..1211
                     /locus_tag="PKZ53_19205"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ42624.1"
                     /translation="MRRLTPFLCVLWLLLGWLTADGLAQTSKKSTPFQSKAVVNQKAT
                     PSPTAGWANLTTIKVASRPAFEFPTLSDDSVAAALSPDGRWLATNYQNASIRLWNLKT
                     GAPGLDLKGHSDMVLSVAFSPDGTRLASGSRDTTIKLWDLSTGKELATLTGHPGDVSS
                     VAFSPDGSLLASAGFGTPWRLWRLSDHQQIGQFDGHLDSIFGLTFSPDGRWVATAGRD
                     KTVKVWDVAKRRIKATFEGHTFWVQAVAFSPDGNLLASASSDGTIRLWNLVKNKPETT
                     LPGQGVGLIAVTFSPDGSWLAVGGGDASVRLWKIATRQSVQPPVRHTGPVRSVFFSPD
                     GTCLITASDDQQVKCWPIEP"
     gene            1370..1780
                     /locus_tag="PKZ53_19210"
     CDS             1370..1780
                     /locus_tag="PKZ53_19210"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNJ42625.1"
                     /translation="MDYSQYEILETIRMTEMENLDIRTATMGISLTDCAGRDIHQVCA
                     SVQEKLLRYASRLVPTVNEIGQEYGLSIANKRLAITPLANVGAACQPQPPDCNRCGLQ
                     QPLVALVSAGRQSGCAARVAPFTARTLSSARSTS"
BASE COUNT          413 a          531 c          499 g          436 t
ORIGIN      
        1 gcgaccagag tgccgggcgc gctaaaaagg gatgactcaa aaaatatcga atgagcgctc
       61 attgtgcgca cttgcctacc tgagtcaact caaaatgtct gaatcccgat caagcgtctc
      121 tttcctctcc tgagcttttt atgttgatgc ggcgtctgac cccattttta tgcgtactct
      181 ggctcttgct gggctggctc acggctgacg gtttggcgca aacttccaaa aagtccaccc
      241 catttcaatc aaaagccgtt gtcaaccaga aagcaacgcc atcacccaca gccggatggg
      301 caaacctgac cacgatcaag gttgcatccc gccccgcatt tgaatttccg acgctgtcag
      361 atgattcggt ggctgccgca ctttccccag atggccgctg gctggccacc aattaccaga
      421 atgcttcaat tcgtctctgg aatctaaaaa ccggcgctcc aggattggac ctcaaaggcc
      481 actccgatat ggttttgtca gtggcgtttt ccccagatgg aaccaggctt gcctctggca
      541 gccgtgatac cacaatcaaa ctctgggacc tctccacagg caaggaacta gccaccctga
      601 ccggtcatcc gggggatgtc agcagtgtgg ccttctcacc agacggcagc ttgctggctt
      661 cagccggatt tggcacgccc tggagactgt ggcgactgag cgaccatcaa caaattggac
      721 agtttgacgg gcatcttgac tcgatctttg ggctgacgtt ctcacccgat ggacgatggg
      781 tggcaacggc tgggcgagat aaaacggtga aagtgtggga tgttgccaaa cgtcggatca
      841 aagcaacctt tgaaggccat accttttggg ttcaggccgt tgcattttct ccagacggaa
      901 acttgctggc ctcagccagt agcgatggca ccatccggct gtggaatctc gtcaaaaaca
      961 aaccggagac cacccttccc ggtcagggtg tgggactcat tgccgtgaca ttttcacccg
     1021 atggaagctg gctggcggtc ggtggcggag atgcctcagt tcgattgtgg aaaattgcca
     1081 cccgacagtc ggtccagcca ccagttcgcc atacaggacc ggttcgaagc gtcttttttt
     1141 cgcccgatgg cacctgcttg atcacagcca gtgatgatca gcaggtaaaa tgctggccga
     1201 ttgagcctta gttcttggtt cttcgtgctt ggtacttggt tcttcgtgcc tggttcttag
     1261 ttcttggtgc ttggtgcctg gttctacttc gaaaatattg attctaagca ccaagtacca
     1321 agcaccaagt accaagtaca atattctttc ccaaaaggaa actctcatca tggactactc
     1381 gcagtatgaa atcttagaga cgattcgcat gaccgagatg gaaaatctcg acatccgaac
     1441 cgccacaatg gggatttcac tcacagattg tgctggacgc gatattcacc aggtttgtgc
     1501 cagcgttcag gaaaaactcc tgcgctatgc cagccggctg gtgccgacgg tgaacgaaat
     1561 cggccaggaa tatggtcttt cgattgccaa taaacggctt gctatcacac ctttggccaa
     1621 tgtcggcgct gcctgccagc cccaaccccc ggattgcaat cggtgcggcc tgcagcagcc
     1681 actggtcgcg ctcgttagcg ccggccggca atctggctgc gcggcgcggg tagcgccgtt
     1741 cacggcgcga acgctttcat cggcgcgatc aacatcgtga cacacaagcg tgacgccgat
     1801 cctgtgcgcc tgacgctcga agggggctat cccacgcttg gcggtcatgg tgaaacggcg
     1861 tggacgtttg gcgcgcatt
//
LOCUS       DAOQDO010001398         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63159_length_1878_cov_3.810203, whole genome shotgun sequence.
ACCESSION   DAOQDO010001398 DAOQDO010000000
VERSION     DAOQDO010001398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63159_length_1878_cov_3.810203"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..>1878
                     /locus_tag="PKZ53_19215"
     CDS             197..>1878
                     /locus_tag="PKZ53_19215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ42626.1"
                     /translation="MSDISPPQEIKDHHIILGAIKSISALEELTEVEPGQGYKFGVDL
                     EVALYGRNYGPGKKVGPYFRLLVYPPGATVLQQGEWETNTFFIVVEGLAQVFIDQRAD
                     PIGEVGVGRPFGEMAVLSGTSQTYTVKAHAQQGMTVLEVQRPALRILRKLQKFGTALD
                     LTYRNKGREFALAALSIPADVRDQLVDIAEFTVLARGHDLCREGAIIDQLILVKDGWV
                     KRIAPDRRHQGDDYSDYLGPGHCLGLNVLEFSEARWPYKVVTLSRTEVLEIPVTELKT
                     RPELLAAVRRACIPFASVGTPLQPGEPLTFSPEAQQAQARILDKGLADATNLLLMDMD
                     LCVRCGNCSRACHMVHHHSRLTRRGIHFVRPQVTLTSTRTQSLLSPAVCLHCKDPECL
                     TGCPTGAIARFAGGKVDIDTKLCIGCGDCATQCPYNAISLIPRTELAPLQPPVKPRAS
                     LPVFSSTRSTSSDSKPHTNTKTGGQTPFNQNPLSSVSSLIGWTQKSQTRTLDPGSQNP
                     NTLHHWLHKLGLKVEPPKLVTSVEDLVAIKCNLCTGTPLNPTDSTGTALYAD"
BASE COUNT          432 a          508 c          486 g          452 t
ORIGIN      
        1 cctgatgagc caacaccaga tatatatgat aaggtaacct cctgagcaat cagggttgtc
       61 tggtgtttga ctggtagact cgtttccttc cctctcaaaa caaactgaac cagccgctac
      121 ttaccgccga aaatgtgggc ggtgtgtgat gtaacttttt gttgtgaatt gggaacaccc
      181 ccggagcctg ttctttatga gtgacatttc cccgccgcag gaaatcaaag atcaccacat
      241 cattcttgga gccattaaat ctatcagtgc cctggaagaa ctgaccgagg tcgaaccagg
      301 ccagggatat aaatttggag ttgacctgga agtggcgctc tatggtcgta actatggccc
      361 tggaaaaaaa gttgggcctt acttccggct actggtttac ccgccaggtg ccactgtgct
      421 ccagcaaggt gaatgggaaa ccaacacctt tttcatcgtg gtggagggac tggcccaggt
      481 ctttattgat caacgggctg atccgattgg tgaagtcggg gtaggccgcc cgtttgggga
      541 aatggccgtt ttgtctggca cctcccaaac ctataccgtc aaggcccatg ctcagcaggg
      601 aatgaccgtg ctcgaagtcc agcggccagc tttacggatt ttgcgcaagc tccaaaaatt
      661 cggaaccgcc ttagacctga cctaccgcaa taaaggtcgt gaatttgcac tggccgccct
      721 atccatcccg gcagacgtgc gggaccagtt ggtggatatt gctgaattta cggttctggc
      781 gcgtggccat gatctatgcc gggaaggcgc catcattgat cagttaattc tggtcaagga
      841 tggctgggtt aaacggattg ccccagaccg gcgtcatcaa ggggatgatt attctgatta
      901 tctagggccc gggcattgtc tgggactcaa cgtgttggaa ttttccgagg cccggtggcc
      961 ctataaagtg gttaccctga gccgaaccga agtcctggaa atcccagtga ctgagttaaa
     1021 aacccgcccg gaattactgg ctgccgtgcg tcgggcctgt atcccgtttg cctcggttgg
     1081 aaccccgctt cagccgggcg agccgctgac attttctccg gaggctcagc aggctcaggc
     1141 gcggatttta gataaagggc tggctgatgc caccaatctc ttgttgatgg acatggattt
     1201 atgtgtccgg tgcggaaatt gctcgcgggc gtgccatatg gtgcatcatc attcacggct
     1261 gacgcgtcgg ggcattcatt ttgtgcggcc acaggtcaca ttgacctcaa cccgcaccca
     1321 gagtttgttg tctccggcag tctgtttaca ctgtaaagat ccggaatgcc tcaccgggtg
     1381 cccaacgggt gccattgccc gctttgctgg tgggaaagtt gatattgaca ccaaattgtg
     1441 tattgggtgt ggcgattgtg cgacgcaatg tccgtacaat gcgatctcgt tgattccacg
     1501 gactgaatta gcaccacttc aaccgccagt caaacccagg gcttcattac cggtattttc
     1561 ttcaacccgc tccacttcct ccgattcaaa accacacacg aatacaaaaa ctggcggaca
     1621 aacacccttc aatcaaaatc ccctgtcgtc agtttcttca ttgatcggct ggacccagaa
     1681 aagccagacc cggaccctgg atcccggatc ccaaaaccca aacacgcttc atcactggct
     1741 acacaaactc ggtctcaagg ttgagccccc aaagctggtg acgtcggtcg aagatctggt
     1801 ggctatcaag tgtaacctct gcactggaac cccacttaac ccaactgatt caaccggaac
     1861 ggcgctctat gcggatca
//
LOCUS       DAOQDO010001399         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63352_length_1875_cov_4.173626, whole genome shotgun sequence.
ACCESSION   DAOQDO010001399 DAOQDO010000000
VERSION     DAOQDO010001399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63352_length_1875_cov_4.173626"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PKZ53_19220"
     CDS             <1..957
                     /locus_tag="PKZ53_19220"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ42627.1"
                     /translation="VGRYVTEQLVQEGHQVRALVRPSTDSAWLQALGVQCVTGTLTNL
                     DSLQAAIDGCQLIYHLAALTSRQNRTASDLWQVNVEGTRNLAQAAAQASVRRLVYVST
                     LGVHGSNFTPPLDEQTPVAPNTGYRRTKWQGEQVVLGIHRLGKLPVVIARIGSIIGPH
                     GTNWLGLMKAIASGRFGYVGNGSNRVQLTYGTDIAQGIIRCGIVPGIEGELFALAGPK
                     SVMLSEFVGHLARELGVSIPQKHSPALPFWLFQQCADRCFSVTGIELPFGHRYNLFFS
                     DYLISIAKAQQRLKYAPVISLEESVHLTVDWTRKHGYLPHPV"
     gene            954..>1875
                     /locus_tag="PKZ53_19225"
     CDS             954..>1875
                     /locus_tag="PKZ53_19225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ42628.1"
                     /translation="MKILLLSDYATLTGGAEIAVAGLRDGLRQRGHDARWLASSARPL
                     GAASHADYECLGSLSQSRILLQTANPWAYWKLRQVLWEFQPDVVDVNLFLTQLSPLIL
                     PLLRNIPALYRAHWYRAVCPTGTKMLPCGNSCQSQAGSNCYHNGCLSRLDWVLLMVQL
                     KLFRRWQSVFQTIIPISQMIREELYRGGIKAQEPVRLGVLKTSSLPAWSPIPTIGFAG
                     RLVPEKGADVLILAFARVQQTLPQARLILIGDGPERRHLEELTATLGLSAAVEFHGHQ
                     LQPATSQRLAQTWIQVVPSRWPEPLGLVAME"
BASE COUNT          414 a          513 c          499 g          449 t
ORIGIN      
        1 gtcggcaggt atgtgaccga gcaacttgtt caggaaggtc atcaggtacg ggcactggtc
       61 cgaccatcaa cggattccgc ctggttgcaa gcactgggcg tgcagtgtgt caccggtact
      121 ctgaccaatc tggattccct gcaggcggca attgatggat gtcaactcat ctaccacctg
      181 gcagcgctca ccagtcgcca aaacagaact gcaagcgact tgtggcaggt taatgtggaa
      241 ggaactcgaa atctggctca agccgcagcc caggccagcg tcaggcgact ggtttatgtc
      301 agtacactgg gtgtacatgg ctcaaatttc acgcctccgc tggatgagca aacaccagtt
      361 gcccccaata ctggctatcg aagaaccaaa tggcagggcg aacaggtcgt gcttgggatt
      421 catcgcctgg ggaaattacc tgtggtgatt gcccggattg gcagtatcat tggcccgcac
      481 ggcaccaact ggcttgggct catgaaagcg attgcatctg gacggtttgg gtatgtggga
      541 aacggatcga atcgggttca attgacttat ggaaccgaca ttgcccaggg catcattcgc
      601 tgtggaattg ttccagggat tgaaggtgaa ctatttgctc tggccggccc gaaatcggtg
      661 atgttgagtg agtttgtggg tcatctggca cgtgaattag gggtctccat accacagaaa
      721 cactcaccag cgcttccctt ctggctcttc caacaatgtg ctgaccgctg cttttcagtt
      781 actgggattg aactcccttt tggtcaccga tataacttgt tcttttcgga ttacttgatt
      841 tcaattgcca aagctcagca acggctcaag tatgctccgg tgatatcact cgaagaatcc
      901 gtccatctca cagtagactg gactcgaaaa catgggtatt tgccgcaccc agtatgaaaa
      961 tcctgctgct ttccgactat gccaccttaa ccggcggtgc tgagattgcc gtcgctggct
     1021 tgcgcgatgg cctccgccaa cgtggtcacg atgcccggtg gctggccagt tcagcacgtc
     1081 cactgggtgc cgccagccat gccgattatg aatgtttggg aagcctctct caaagtcgaa
     1141 tcctgctcca aaccgccaac ccctgggcgt actggaaact cagacaggtc ctctgggaat
     1201 ttcagccgga tgtggttgat gtcaaccttt ttctgactca gctttcaccc ttgattttac
     1261 cgctcttgcg caatattccg gcactgtatc gagcccactg gtaccgggca gtttgcccaa
     1321 ccggcacgaa aatgctccct tgcggcaatt cttgccagtc tcaagccgga tccaattgtt
     1381 atcacaatgg ctgcctttcc cgacttgact gggtactgtt gatggtacag ttaaaactct
     1441 tcaggcgctg gcaatcagta tttcaaacca tcattccaat cagtcaaatg attcgagaag
     1501 aactctatcg cggggggatc aaggcgcagg aaccagtcag attgggggtt ctcaaaacct
     1561 catccctgcc tgcctggtcg cccattccaa ccattggctt tgcaggccgc ttggttccgg
     1621 aaaaaggggc ggatgtcctg attttggctt ttgccagagt gcagcaaacg ctcccacaag
     1681 cccggttgat actgatcgga gacggcccag aacgcagaca cctggaagaa ttaaccgcca
     1741 cactcggact ttcggcagcc gttgagtttc atggccacca gttgcagccg gcgaccagcc
     1801 agcgattggc ccagacctgg attcaggtgg ttccgtcacg ctggcccgaa ccgctgggac
     1861 tggtcgcgat ggagg
//
LOCUS       DAOQDO010001400         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63380_length_1875_cov_2.498352, whole genome shotgun sequence.
ACCESSION   DAOQDO010001400 DAOQDO010000000
VERSION     DAOQDO010001400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63380_length_1875_cov_2.498352"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PKZ53_19230"
     CDS             complement(<1..1468)
                     /locus_tag="PKZ53_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42629.1"
                     /translation="MSPHEQLQIEELLSAGELQQAFSVIGTFSTLKQSEIPSLSKDIL
                     LRLETTDGMSHQFVLGLMSPSVSVRRFCRKYLPKLGNSAAKFLYFHLIEALDPFRPVL
                     RPQDGLAGSYAGFASPIAQHIIEEEYSQTPLRNSQYTEAHRYAMDCIEMMASTLIAIR
                     PDLGFLLGEYLIGFAQLPVEQTFQANSGRDRQTLQFYQQALEETLEPAWWGLIKKNDA
                     QAVKAAQTWKQHQWLIDAEPWSNEAILKPVRPLYSRFNAYQALIFQQLEEKSTLALQG
                     VSHTTVQETRPKVKPLAAVKKPALPQLPGVAAVPAGPAIKLLTNRLKLFIDVLKTQIG
                     RKKGGRARFLDLTKISLPESGVRTDLRKYLAQPRPRDFISELDLAKAPWANDFLKLLI
                     ETAKPQAAKIKHRRGNKTLPAWSDFSEFRDFFFEVTWDLEQLNYVAGMFLPETQLETW
                     VEFWRTEIGLYGPTFDLWPPMLEIREQRGILADSFFSPW"
     gene            complement(1549..>1875)
                     /locus_tag="PKZ53_19235"
     CDS             complement(1549..>1875)
                     /locus_tag="PKZ53_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HNJ42630.1"
                     /translation="KGKELMHHIGRTLLVHHKMVGRVEVDEKPELLWERPELERLYLR
                     LEDEYELKERHVALERKMELIFRTVETLLDIQHSNRSLRLEWYVIILIVVEILLTLYE
                     MFVKHR"
BASE COUNT          452 a          465 c          472 g          486 t
ORIGIN      
        1 accagggaga gaaaaacgaa tccgccagaa tcccgcgctg ctcacgaatt tcgagcattg
       61 gtggccagag gtcaaaggtt ggtccataaa ggccaatttc ggttcgccaa aactcaaccc
      121 aggtttccag ttgggtctca ggcaggaaca tgcccgccac gtagttgagt tgctccagat
      181 cccaggtgac ttcgaaaaag aaatctcgaa attcagaaaa atctgaccac gccgggagcg
      241 ttttatttcc gcgacggtgt ttgattttag cggcctgtgg tttggcggtt tcaatcaaca
      301 acttcaaaaa atcattggcc cagggagctt tggccagatc cagttcactg atgaagtctc
      361 gcgggcgggg ttgcgccaga tattttcgca gatccgttcg aaccccagac tcgggcaacg
      421 aaattttggt caaatccaga aatctggccc gtccgccttt tttccggcca atttgggttt
      481 tgagcacatc aataaacaac ttgaggcgat tggtcaggag cttgatcgcc ggtccggctg
      541 gcaccgctgc cacacctggg agttgaggca gtgcaggttt tttgactgct gccagcggtt
      601 ttactttggg acgggtttcc tggaccgtcg tatgtgaaac accctggagc gccagtgttg
      661 atttctcctc aagttgttga aatatcagtg cttgatacgc attaaatcgg gaatacaggg
      721 gtcgaaccgg tttcagaatg gcttcattac tccagggttc ggcgtcaatc aaccactgat
      781 gttgtttcca ggtctgggcg gctttgaccg cctgggcgtc atttttcttg atcagccccc
      841 accaggctgg ttcgagcgtt tcttccaatg cctgctgata aaactggagt gtttggcgat
      901 ctcgcccgga gtttgcctga aaagtttgtt caactggaag ctgggcaaac ccgatcagat
      961 attcccccag caagaatccc aaatctggac gaatcgcaat caacgtcgac gccatcatct
     1021 cgatacaatc catggcatac cggtgggctt ccgtatactg actgttgcgt agcggcgtct
     1081 gggaatactc ttcctcgata atatgctggg ctatcggact ggcaaacccg gcgtaacttc
     1141 cggccagtcc gtcctggggt ctcaacacgg gtcgaaatgg gtcaagtgcc tcgatcaaat
     1201 gaaaatacag gaatttcgcc gcgctgtttc caagctttgg caggtatttt cgacaaaatc
     1261 ggcgaactga aaccgaaggc gacatcagcc ccaatacaaa ctgatgactc attccatcag
     1321 tggtttcaag tcgcagtaaa atgtctttgg aaagtgacgg aatttcgctt tgcttgaggg
     1381 ttgaaaaggt tccaataacc gaaaaagcct gttggagctc accagcagac agtaactctt
     1441 cgatctgcaa ttgttcgtgt ggtgacatgg cgggtgcgtt cctcagtaaa caaaatagaa
     1501 ttcgattacc gagtaatagc ataactccgt aattttgcaa actgtgtttt aacgatgttt
     1561 gacgaacatc tcatacagcg ttagcaaaat ttcgaccaca atcaaaataa tcacgtacca
     1621 ttcgagccgg agcgaccggt tgctgtgttg aatatcgagc agggtttcaa ctgtacggaa
     1681 gatgagttcc atttttcgtt ccagggccac gtggcgttcc ttgagttcat attcatcttc
     1741 gagccgcaga tagaggcgtt ccagttcggg gcgttcccag agcagctcag gtttttcatc
     1801 cacttcgacc cggcccacca ttttgtgatg caccagcagg gtccgaccaa tgtggtgcat
     1861 caattctttt ccttt
//
LOCUS       DAOQDO010001401         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63436_length_1874_cov_2.660803, whole genome shotgun sequence.
ACCESSION   DAOQDO010001401 DAOQDO010000000
VERSION     DAOQDO010001401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63436_length_1874_cov_2.660803"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1022
                     /locus_tag="PKZ53_19240"
     CDS             309..1022
                     /locus_tag="PKZ53_19240"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNJ42631.1"
                     /translation="MGSFRLSTGKLSLLIIASAWLVGATTTIRAQELTPTLSSATPDS
                     ATRPRRAVITEETLKTAKPETLSDENESEESARPKSRVFTNSDFPSGKAVPTNRLDGL
                     GPELQRLQASIDTKLGFPYRYHSQGEGAYDCSGFVWSVFRDIGVSFTRSCAAEYYHAF
                     PPATAEQKTQFGTLIFFNGLGHIGIVKDANHFYHASTSAGTTLTKLNDYWKARITGYR
                     VVPGLVAPSPELSASSNEE"
     gene            1408..>1874
                     /locus_tag="PKZ53_19245"
     CDS             1408..>1874
                     /locus_tag="PKZ53_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42632.1"
                     /translation="MSAKQNRNRPRTTQADQIPQSPLDREANVIRIDSPGVQIGYNQR
                     IRATKIEFRSPDDPQTGKLPNGLPHRGDAKVETKGGFTEIDVQGNIVLPGPAGGIDPK
                     LNTYVLWSITPTGTVQNLSEVLYEDDLGGAGSDAREANVTTTQPVFALMITAEP"
BASE COUNT          473 a          508 c          447 g          446 t
ORIGIN      
        1 gaacccggaa aacggtaaaa agcgactgga attaccgcag agagcttgcc gagcggcagg
       61 actttgagta acatcccgcc cgttcttcaa tcaaggttca ttcctttgtg tgaaacatcc
      121 actacctgag tggttcctgt ttcaccagtt caaggttctc aatcaattga ttctcgcgtt
      181 cgtctgaggt gagccgccgg gtggtttctg ctttcatccg ccttgtgtgt cttgtgtgcc
      241 tcaactaact gatttttgca agtgggactt tgcgttcccc tgcgtttttc cagggaggta
      301 tgtgcttcat gggatctttt cgtttgtcca ccggcaaact cagcttgttg atcatcgcca
      361 gtgcctggct tgttggcgcc accacaacca tcagagcaca ggaattgacc ccgactttat
      421 catcggccac gcctgattcc gccacccgac cacgacgcgc cgtgattact gaagaaaccc
      481 tcaaaaccgc caaacccgaa accctttcag acgaaaacga atcggaagaa tcggccaggc
      541 ccaaatccag agtgtttacc aattcagact ttccttcggg aaaagctgtt ccaaccaacc
      601 ggcttgatgg attgggacca gaattgcagc ggttgcaggc ttcaattgat accaaacttg
      661 gattcccgta ccgctatcat tcacagggtg aaggcgccta tgactgctct ggatttgtct
      721 ggagcgtctt tcgagacatt ggggtttcat ttacccggag ctgtgcggct gagtactatc
      781 atgccttccc acccgcaacg gctgagcaaa agacacaatt tggaacgctg attttcttca
      841 atgggttagg gcacattggt attgtcaagg atgccaatca tttttatcac gcctcaacca
      901 gcgccggaac gaccctcacc aaactcaatg attattggaa agcccgcatc acaggctatc
      961 gggttgtgcc tggattggtc gccccgtcgc cggaactgtc ggccagcagc aatgaggaat
     1021 agccagcaat ccccacgaga tcatcaaagt atgaacacca aaaatgccgc cggaatgtgg
     1081 ttcgggcggc gttttctgtt tttcaagcaa ccaacctgag tgaagtggaa tcgggtttag
     1141 ggtttagggt tccgggtttt caaaaccaag aaccaagaac caagaaccaa gaaccaggca
     1201 ccaggcacca gtcaccagga atcaggcacg agtcaccaga ttctgtaatc aatcaccaca
     1261 aatccctcat ttaccctgta cctataacat cacacttatc acacgaggag ccatgcgttt
     1321 atgacctatc atcttttggc gaaatcaagg cttcaatcct tatttgcttt gtttttcatc
     1381 agtttattgg tttttggaac tttgcctttg tctgcaaaac aaaaccgtaa ccgcccacgg
     1441 acaacacagg ctgatcagat tccgcagtca ccgctggatc gtgaagcgaa tgtgatccga
     1501 attgattcac ctggagtcca aatcgggtat aaccagcgga ttcgagccac gaaaatcgaa
     1561 tttcgcagcc ccgacgatcc ccaaaccgga aaacttccca atgggctgcc gcaccggggc
     1621 gatgccaaag ttgaaaccaa aggcggattc accgaaattg acgtgcaggg caacattgtg
     1681 ctgccggggc cggctggcgg gattgatccg aaattaaata cctatgtgct gtggtcaatt
     1741 accccgaccg gtactgtgca gaatctgagc gaagtgctgt atgaagacga tcttggcggc
     1801 gccggcagcg atgcccgcga agcaaacgta accacgaccc agccagtatt tgccctgatg
     1861 atcaccgctg aacc
//
LOCUS       DAOQDO010001402         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63489_length_1873_cov_3.548955, whole genome shotgun sequence.
ACCESSION   DAOQDO010001402 DAOQDO010000000
VERSION     DAOQDO010001402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63489_length_1873_cov_3.548955"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..92
                     /locus_tag="PKZ53_19250"
     CDS             6..92
                     /locus_tag="PKZ53_19250"
                     /inference="COORDINATES: protein motif:HMM:NF025274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HNJ42633.1"
                     /translation="MRLVVWMALGIVIYFTYGIHHSKIQKVQ"
     gene            331..600
                     /locus_tag="PKZ53_19255"
     CDS             331..600
                     /locus_tag="PKZ53_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42634.1"
                     /translation="MSPSARTSTAPSLLQICLTTIHTVAIDAVQLLCSIDFGVSFKPH
                     PTGLDCGFVFGVTASENSTHLPLAAPTCLVRRDPASVLGARAGYL"
     gene            907..>1873
                     /gene="tal"
                     /locus_tag="PKZ53_19260"
     CDS             907..>1873
                     /gene="tal"
                     /locus_tag="PKZ53_19260"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNJ42635.1"
                     /translation="MNPLKALHEYKQSPWLDYIRRSLITSGELQRLIDEDGLMGLTSN
                     PAIFEKAITGSNDYTDAIAELESENLEATELYERLAIRDIQDTADSLRSVYEKTSRRD
                     GYVSLEVSPYLARDTQGTIDEARRLWKAVNRENLMVKVPATTEGVPAIKQLISEGINI
                     NVTLLFAQAAYEAVAEAYISGLETFVANGGDPGHVASVASFFISRIDTAIDALAQAKL
                     QATTDPAEQARIKSVMGKVAIANAKITYQAYKEIYHSKRWQALAEKGAQSQRLLWAST
                     STKNPSFRDVIYVEELIGADTVNTVPPATFDAFRDHGKLRASLEED"
BASE COUNT          434 a          568 c          439 g          432 t
ORIGIN      
        1 cctggatccg actggtggtc tggatggccc tcggcattgt gatttatttc acctacggca
       61 ttcaccacag caaaatccag aaagtgcagt aagtcccctg gcttttcccc tggcaggtga
      121 cggtcccgtc acctgccacg ccgggttctt ttccatccag ttctgtccca cccgtggttc
      181 cccaccaatc tcattcctca tattttgctt gcctttcagt cggttgtgct ggtacaacac
      241 gcctaccatc ttcatcttca cacctgttct cattcaattc caattcacgg tcattcgcgc
      301 tcagaagcgt tcaaggggga gtctcaagtc atgtcaccat cggcacggac ctcaaccgcg
      361 ccttcgctgc tgcagatttg ccttactacc atccacaccg tggcgattga cgccgttcag
      421 ttattgtgtt ccatcgactt tggggtttct ttcaagccgc acccaaccgg tctggactgt
      481 ggatttgtgt ttggagtcac cgcttccgaa aattcaaccc acctgccgct tgccgccccg
      541 acctgtctgg tcaggaggga tcctgcctcc gttttgggag cacgcgctgg ttatctatga
      601 aggtgcccac atcgcctttt cagcacgtga tgacccaact tttccggaat agacctggaa
      661 cagcctggat tcacggctga gcacatcatc aatcgctctt acaccttcct gtgaagtaag
      721 aattatgact gatgaaacga actgacaggc aaaatgcggt cttgcccttg cgattatgcc
      781 ctggtgaatc cgctggtcgt ggatcaacct cccttgaccc atcgccagcc atcaccgctc
      841 ggttcatact ttacaccaca tcgtttccgt taacctttgg tgagcacaag aaaggcagag
      901 tattccatga atcccctgaa ggctttacac gaatataaac aatctccgtg gctggactac
      961 atccgacgga gcctgattac cagtggtgaa ctccaacgcc tgatcgatga agatgggctg
     1021 atggggttga cctcaaatcc cgcgattttt gaaaaagcca ttaccggcag taatgattac
     1081 actgatgcca ttgccgaact cgaatctgaa aatcttgaag cgaccgaact ctacgagcgt
     1141 ttagccattc gcgacatcca ggacaccgcc gatagcctgc ggtcagttta tgaaaaaacc
     1201 agccgccgcg atggatacgt cagtctcgaa gtttccccgt acctcgcccg cgatacccag
     1261 gggaccattg acgaagcgcg tcggctctgg aaagccgtca atcgggagaa cttgatggtc
     1321 aaagttccgg ccacgacaga gggtgtccca gccatcaagc aactgatcag tgaaggaatc
     1381 aacatcaatg tgaccctgct ttttgcccag gcagcctatg aagccgttgc cgaagcctat
     1441 atctccggcc ttgaaacgtt tgtcgccaat gggggcgacc caggccatgt cgccagcgtc
     1501 gccagcttct ttatcagccg gattgatacc gccattgatg ccctggccca ggccaaactg
     1561 caagcaacca ctgatcccgc cgagcaggcc cgaatcaaaa gcgtgatggg caaagtcgcg
     1621 attgccaacg ccaaaatcac ctatcaggcg tacaaagaaa tttatcactc caaacgctgg
     1681 caggccctgg ccgaaaaagg cgcccaaagc cagcgcctcc tctgggccag caccagcacc
     1741 aaaaacccca gcttccgtga tgtgatttat gtcgaagaat tgatcggtgc cgataccgtc
     1801 aataccgtcc cgccagccac ctttgatgcc ttccgcgatc acgggaaact gcgggccagc
     1861 ctcgaagaag atg
//
LOCUS       DAOQDO010001403         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63510_length_1873_cov_2.697470, whole genome shotgun sequence.
ACCESSION   DAOQDO010001403 DAOQDO010000000
VERSION     DAOQDO010001403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63510_length_1873_cov_2.697470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKZ53_19265"
     CDS             <1..660
                     /locus_tag="PKZ53_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42636.1"
                     /translation="RILNFRVTVRDNRTVGGISSNLMRVNVTNKSGPFEVTSPNVALR
                     WQIGTTQNVTWNVAGTTADPVNCAFVNIRLSTDGGLTYPITLAENTANDGTEPITVPN
                     AETSQARIKIEAVGNIFFDVSNADITLTSGTVPAVEVTAASFQPKKLTVTGVGFGLTP
                     IMIVNGVELPESLLLPGSTENQVTAKGGRKKLSLRKGANTIQIKGTGGQLSNVFGFQV
                     E"
     gene            779..>1873
                     /gene="lipB"
                     /locus_tag="PKZ53_19270"
     CDS             779..>1873
                     /gene="lipB"
                     /locus_tag="PKZ53_19270"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNJ42637.1"
                     /translation="MTEQFSPSGTDRELLVWSLGEVDYVDGLRLQEVLVDLRKEGKIP
                     DALVFLEHPPVLTLGRGADKTNIVATPTFLEGAGIEVHETGRGGEVTFHGPGQLIGYP
                     IVDLNPDRRDVRRYMRDLEQVIIAALAELGIESGRIDGLTGVWVGSRKIAAMGVRISR
                     WVTSHGFALNVNTALDYFNLIVPCGIRDKGVTSIAEVLGRDVEFSEVEPLILKHFGEV
                     FGRTITPKTIEHESIQVVIGHRHKNKVEYLLLHRVPESGGFWQPVTGTIERKRNETPF
                     EAANREVFEETGFELHITPLDYVHSFMIDPIYQKKGMTSPIFNREHSFTARAPHREVR
                     LAVNEHSEYEWLDYESAKKRLMWRGNHRAFE"
BASE COUNT          460 a          442 c          534 g          437 t
ORIGIN      
        1 cggatcctca atttccgggt aaccgttcgc gacaaccgca ccgttggcgg tatcagcagc
       61 aatttaatgc gggtcaatgt caccaacaaa agtggaccat ttgaagtgac gtcacccaat
      121 gtcgcattgc ggtggcagat tgggaccacc caaaacgtga cctggaatgt cgccggaact
      181 acggctgatc cggtcaattg cgcctttgtg aatattcggc tttcaaccga tggaggactg
      241 acctatccaa taacgttggc tgaaaatacc gccaatgatg gcactgagcc gattacggtt
      301 cccaatgccg agaccagcca ggctcgaatc aaaattgaag ccgttggcaa tatctttttc
      361 gatgtttcaa acgcggatat cacgttgacc tcgggaacag ttccagccgt tgaggtcacc
      421 gccgccagct ttcaaccgaa aaagttaacg gtgaccggtg tggggtttgg gctgactccg
      481 atcatgattg taaatggggt cgaattaccc gaatcattgt tgcttccagg gtcaactgaa
      541 aatcaggtta cggcaaaagg cgggaggaaa aagttgagtc ttcgcaaagg agccaacaca
      601 atccagatca aaggcacagg tgggcagctg tccaatgtct ttggttttca ggtcgagtga
      661 tttgttgagg gcttggggct gagggctttg ggctaagggt aaaagcaatt cttccaactg
      721 tttactcatt gcataatcct gtggatttga tcccgaagta gtatgagagg actccgagat
      781 gactgaacaa ttttcgccgt caggaaccga ccgtgaactg ctggtctggt cgctggggga
      841 agttgattat gtggatgggc tgcggttgca ggaggttctg gttgaccttc gcaaagaagg
      901 aaaaatcccg gatgccctgg tgtttctgga acatccgccg gtactgaccc tggggcgtgg
      961 tgctgataaa accaatattg tcgccacgcc gacatttctc gaaggagccg gaatcgaagt
     1021 ccacgaaact ggacggggcg gggaagtcac gtttcacggt ccagggcaac tgattgggta
     1081 tccgattgtt gatttaaacc cggatcggcg tgatgtgcgg cggtatatgc gcgatctgga
     1141 gcaggtcatc attgccgctc tggccgaact ggggattgaa tcgggccgaa ttgacggcct
     1201 gaccggcgtg tgggttggtt cacgaaaaat cgcggccatg ggggtgcgga tttcccggtg
     1261 ggtgacttcg cacgggtttg cactgaacgt caacacagca cttgattact tcaacctgat
     1321 cgtgccctgt ggcattcgag acaaaggcgt gacttcaatc gccgaagtgt tgggccgtga
     1381 tgtcgaattc tcagaggtcg aaccattgat tctgaagcat ttcggcgagg tttttggtcg
     1441 gacaattacg ccgaagacaa ttgagcacga atccatccag gtggtgattg gccatcggca
     1501 caaaaacaag gtcgaatatt tattgctgca ccgtgttcca gaaagcgggg gcttctggca
     1561 accggtcacc ggcaccatcg aacgcaaacg caacgaaacg ccgtttgaag ccgcgaaccg
     1621 cgaagtcttt gaagaaaccg ggtttgaact ccacattacc ccgctggatt atgttcacag
     1681 ctttatgatt gatccgattt accagaagaa aggtatgaca tcgccgattt tcaaccggga
     1741 acattctttt actgcccggg cgccgcatcg cgaagtgcgg cttgccgtaa acgaacacag
     1801 cgagtacgag tggttggatt acgaatcagc caaaaagcga ttgatgtggc gcggcaatca
     1861 tcgagccttt gaa
//
LOCUS       DAOQDO010001404         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63630_length_1871_cov_3.440529, whole genome shotgun sequence.
ACCESSION   DAOQDO010001404 DAOQDO010000000
VERSION     DAOQDO010001404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63630_length_1871_cov_3.440529"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1871
                     /locus_tag="PKZ53_19275"
     CDS             78..>1871
                     /locus_tag="PKZ53_19275"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ42638.1"
                     /translation="MAAHLPTQTRFGPYEIIGKLGAGGMGAVYRARDTRLGRDVAIKI
                     LSPEAATNPELKRRFENEARAASALNHPNIVAIYDVGFEHDQSYIVSELVEGRSLRAL
                     MHEGTLTLNQMLHLVIQVADGLTAAHEAGIVHRDLKPENIMVSRDGRAKILDFGLAKL
                     IPTIPQSQNSQINTQIIDESQPPQVRTTPGVILGTVSYMSPEQASGDPIGFHSDQFSF
                     GVILYEMISGKRPFDRANTIQTLAAIITDPAPPLQESDQRLPEAFCQLIARCLAKNPG
                     KRFPTTRDLMEELQTIHSLCSGESAITLRLWDSTPPITKHAWGLPTRHLRTGLFGLIV
                     TLLLAALVFPAVKYFRERAKARNVVENSAIDSIAALPFVNTISDPSTEYLSDGITENI
                     INSLSHVPGLRVMARSTVFRYKGSEVDPQKVGQELKVRSVLTGRVTQRNKMLVISTEL
                     VDVKDGSRLWGEQYNSTTSDILFTQDEIAEDISTNLQFRLTGEQRQKVIKHFTENSEA
                     HQAYLRGRYAWNKRTKESLESAIEHFKQAIRIDPNYALAYAGLADTYGLMGYYGLRRP
                     REAYPEAKEAVLKALELDPNIAEAHASLADIK"
BASE COUNT          470 a          530 c          455 g          416 t
ORIGIN      
        1 acttttcatt cgcgaaaacc cggaacccgg aacccggaac ccggaaccct gtttttcaaa
       61 ccctgaaccc tgaaaaaatg gcagcccacc ttccaacaca aacccggttc ggtccttatg
      121 aaatcatcgg caaacttggt gccggcggta tgggagcggt ctatcgagcc cgtgataccc
      181 ggctcgggcg tgatgtggcg atcaagattt tatcacccga ggcggccacc aatcctgaac
      241 tgaagcgccg ctttgaaaat gaagcccggg ccgccagtgc gctcaatcat ccaaatattg
      301 tcgccatcta tgatgttggg tttgaacatg accagagcta tattgtctcg gaactggtcg
      361 aggggcgctc tctgcgtgcg ctgatgcatg agggcacatt gacgctcaac cagatgctcc
      421 atctggtaat ccaggtcgcc gatggactga cggcagcgca tgaagccggg attgtccacc
      481 gtgatctcaa acccgaaaat atcatggtca gccgcgatgg acgcgccaaa attttggact
      541 ttgggctggc aaagctcatt cccacgattc cccaatccca aaattcacaa atcaataccc
      601 agatcattga tgaatcacag ccaccgcagg ttcggactac acctggggtc atcctgggaa
      661 cggtcagcta tatgagcccc gaacaggcat caggtgatcc aatcgggttt cattccgatc
      721 agttttcatt tggagtcatt ctgtatgaaa tgatcagcgg caagcgccca tttgaccggg
      781 ccaacacgat tcaaaccctg gccgccatta ttaccgaccc ggcgccaccg cttcaggaat
      841 ctgatcaacg cctgcctgaa gcattctgcc agttgattgc ccgatgtctg gccaaaaacc
      901 ctggaaaacg cttcccgacc actcgcgatt taatggagga attgcaaacc atccacagcc
      961 tgtgttccgg cgagagcgcc atcaccttac gcctttggga ttcaacgccg cccatcacca
     1021 aacacgcctg gggcctcccc acccgtcatc tgcggaccgg tttatttgga ctcatcgtga
     1081 ctttgttgct ggcggcgctg gtttttccgg cggtgaaata ctttcgcgaa cgcgccaaag
     1141 cccgcaatgt ggttgagaat tcggcgattg attcgattgc ggcgctgcca tttgtcaata
     1201 ccatttctga tccttcaact gagtacctga gtgatggcat cactgaaaac atcatcaaca
     1261 gcctgtcaca cgtacctgga ttgagagtca tggcacgtag caccgtcttt cgctacaagg
     1321 gcagcgaagt tgatcctcaa aaagttggtc aggaattaaa agtccgctcc gttttgaccg
     1381 ggcgcgtcac ccaacgcaac aaaatgctgg tcatttcaac ggaactcgtg gatgtgaaag
     1441 atggttcgcg gctctggggc gagcaataca acagcaccac ctctgacatt ctgtttaccc
     1501 aggatgaaat tgcagaagat atctccacca accttcaatt ccggctgacg ggtgaacagc
     1561 gtcagaaagt catcaagcat tttactgaaa attcagaagc ccatcaggcc tacctgcgcg
     1621 gacgctacgc atggaacaaa cgcaccaaag agtcactcga atcagccatt gagcatttca
     1681 aacaggcgat tcgcattgat ccaaactatg ccctggccta tgccgggctg gctgatacct
     1741 atggattgat gggttactac ggcctgcgtc ggcctcggga agcctatccg gaagctaaag
     1801 aagcggtgct caaagctttg gagttagatc caaacattgc cgaagcccat gcttcactgg
     1861 cagatatcaa a
//
LOCUS       DAOQDO010001405         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63635_length_1871_cov_3.062225, whole genome shotgun sequence.
ACCESSION   DAOQDO010001405 DAOQDO010000000
VERSION     DAOQDO010001405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63635_length_1871_cov_3.062225"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1247)
                     /locus_tag="PKZ53_19280"
     CDS             complement(114..1247)
                     /locus_tag="PKZ53_19280"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNJ42639.1"
                     /translation="MTSRIGILGGGQLARMTAYAAFRLGFQIGILEREAESPAAFLTP
                     HSWVGSWNDQQLLDELATYADVVTLENEFVDTEALFYLESRGVHLYPSARTLSTIQDK
                     FLQKTALKRAGLPVPRFAAVESPAEIVQCASSFGWPLMLKARRNGYDGYGNELLRTPE
                     DVIPAFEKLAKRSGLLMVEEFVPFTKELAIMVARSSHGDETAYPVVETIQHAHMCHTV
                     LAPAAISENSIRRASELGKRIMTSLDAVGIFGIELFLMTDGDLMINEIAPRPHNSGHF
                     SIEGCFTSQFENHVRSIVGLPLGSTQMVAPAAVMVNVLGTNDGPAQPKGLIEALSIPG
                     AHVHLYGKKMSRPGRKMGHVTATGHTVDQAREVALRAARLITI"
     gene            1514..>1871
                     /locus_tag="PKZ53_19285"
     CDS             1514..>1871
                     /locus_tag="PKZ53_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42640.1"
                     /translation="MPSSRHRNVERKRKSAASRAIKVSPTYRNEDSLSGGGGGVSGAS
                     VKSGATSESGGPNWMILGVVGLVVVVLVGLYMTSGPKPAPAAAPTAATTPPAQPATPP
                     KNEIVSLPANKEPEPTT"
BASE COUNT          482 a          469 c          483 g          437 t
ORIGIN      
        1 gatcagtatt gaattttttt ctagctttca gagtatactc taacaccacc aaaatgtctt
       61 gggtcaacag gttagagaac cttcactgtt gagtttcttg gcctgagctt tatctaaatg
      121 gtgattaacc tggcagcccg aagtgcaacc tcacgggcct ggtccacagt atggcccgtg
      181 gctgtaacat gtcccatctt ccggcctggg cgggacatct ttttgccgta gaggtgtacg
      241 tgggcacctg ggatggaaag ggcttcaatg agacctttgg gttgggccgg tccgtcgttg
      301 gtaccaagga cattcaccat aactgctgcc ggagcaacca tttgggtaga tccaaggggc
      361 agaccgacga ttgatcgaac gtgattctca aattgggaag tgaaacatcc ttcaattgaa
      421 aaatggcctg aattatgcgg gcgtggggca atctcgttga tcataagatc gccatcggtc
      481 attaaaaaga gttcaattcc gaaaattcca accgcatcca gactggtcat gattcgtttg
      541 ccaagttcag atgcccggcg aatcgaattt tcactaatgg cggctggggc aagtacggtg
      601 tggcacatat gcgcatgctg gatcgtttcc accaccggat aggcggtttc atcgccgtgc
      661 gacgaacgcg ccaccataat ggccagctct ttggtgaagg gcacaaattc ctcaaccatc
      721 agcaacccgc tccgtttcgc cagtttttca aaggctggga tcacatcttc tggggttctg
      781 agcagctcgt tgccatatcc atcataaccg ttgcgccggg ctttgagcat caatggccag
      841 ccaaacgaac tggcacactg cacgatttca gccggggact caaccgccgc aaaccgggga
      901 actgggaggc cagctcgttt aagggccgtt ttttgaagaa atttgtcctg aatggttgaa
      961 agcgtccggg cagaagggta gagatgaacc ccgcgtgatt caaggtagaa gagcgcttcc
     1021 gtgtcaacaa attcattttc cagtgtgaca acatcggcat aagttgcgag ttcatcgagc
     1081 aactgttgat cgttccagct tccaacccag gagtgcgggg tcaggaacgc cgccgggctc
     1141 tcagcctcgc gctccaaaat accaatctga aaaccaagtc ggaaagccgc ataggccgtc
     1201 attcgggcca gttgtccacc gcccaggata ccaattcgtg aggtcataaa agtaaagtaa
     1261 agcggaagtc acatcccaaa aaaatgaaaa tcaggagtga gaatggaaat atgattgaaa
     1321 attcagagta ttcagcagtt gaaaagctga aagggatgtg aaatatagta cactttgttt
     1381 ttcagccgaa agccaaagac cataccgttg tcttccaaat tcatcaccaa atccgaaaaa
     1441 cgttgagtgc tcagggggtt agctcactga gtaccacgag attgattttt gccttgagga
     1501 gtgttttcca attatgccaa gcagtcgtca tcgaaatgtt gaacgcaaac gtaaatcagc
     1561 cgcttcgcgt gccatcaaag tgtctccgac ctaccgcaat gaagattcat tgagcggggg
     1621 cggaggcggt gtttcaggcg ccagcgtcaa aagtggtgct acatcagaat caggcggccc
     1681 aaactggatg attttgggtg ttgtgggtct ggtcgtcgtg gtacttgtgg gtctgtatat
     1741 gacctctggg cctaaaccag ctccagccgc cgccccaact gctgccacaa cgccgccggc
     1801 ccaacccgcc acccctccga aaaatgagat tgtttcgttg ccagccaaca aagagcctga
     1861 accgaccaca c
//
LOCUS       DAOQDO010001406         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63652_length_1871_cov_2.288546, whole genome shotgun sequence.
ACCESSION   DAOQDO010001406 DAOQDO010000000
VERSION     DAOQDO010001406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63652_length_1871_cov_2.288546"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1871)
                     /locus_tag="PKZ53_19290"
     CDS             complement(<1..>1871)
                     /locus_tag="PKZ53_19290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNJ42641.1"
                     /translation="VGTISSGLFLGRRQSNRMVFTNYRRDPKDPHSLGNNAILALLED
                     SHGTVWVGTYQGLYALADSTSDQPKAVFTAYRHRPDDPGSLSSDMIEALAEGPDRHIW
                     IGTGSGLNRLDPSTGQMTRYLHDPNNPASINHEFIDCLYRASTGLIWVGTRAGLCWID
                     PTTGKLSRVPVETGLAEFAIMSVVEDASGAFWVGTINGLFRFDPPTGHTLRFGKNDGL
                     PHELLDRGVVCSGKGGRLYFGTPEGVISFRPEEITQNPMPPSVVLTELLLGNQIQPIR
                     AQSTLPAQINLLETLVLQHTDNLFTIEFSALNFKQPEDNRYAYKLEGLYNDWITTDAK
                     NRRATFTNLEHGEYVFRVKAANNDGVWNEAGPMLKIVILPPWWKTWWARLIFYTTGIL
                     ILLAVPVIRLVISERQRVVLETQVQQRTLELSQANQKLMELDRYKQAMMSMIVHDLKN
                     PLSAILHDGTDKPPEHRLQQIKRFATQMLYLVLNILDVQKFQDAKFELNLQGCRLADI
                     FHQAIDDVDFLRKNKGQIFEIEVDETLEIQVDEAILRRVIVNLLTNAIKFTPVGGTIT
                     VKGILDATDSLRLTIQDSGEGIPKEKQALIFEPYGQVQARASGSVRSTGLGLAFCKL"
BASE COUNT          459 a          486 c          457 g          469 t
ORIGIN      
        1 ccagtttgca aaatgccagc cctaacccgg ttgatcggac actgcctgag gctcgggcct
       61 gaacctggcc atagggctcg aaaatcagcg cctgtttttc tttaggaatc ccttccccgc
      121 tatcctgaat cgtgagccgg agactgtcag tggcgtccag aatccctttc acagttattg
      181 ttccaccaac tggcgtgaat ttaattgcat tcgtcagtaa gttgacgatc acgcggcgca
      241 aaatggcttc atccacctgg atttcaagtg tttcatccac ttcgatttca aaaatctgac
      301 ctttgttttt gcgcaaaaag tcaacatcat caatcgcttg atgaaaaata tccgccagcc
      361 ggcatccctg aagattgagt tcaaatttgg catcctgaaa tttctggacg tccagaatgt
      421 taagcaccag atagagcatc tgggtggcaa accgcttgat ttgctgtaat cggtgctctg
      481 gtggcttgtc ggtaccgtcg tgcaaaatcg cgctcaacgg gtttttcagg tcatgcacga
      541 tcatactcat catggcctgt ttataccgat ccagctccat caatttttga ttggcctggc
      601 tgagttcgag cgtgcgttgc tggacctggg tttcgagcac cacccgctgg cgttccgaaa
      661 tgaccagtcg aattactggt accgccagca aaatcaaaat gcccgtggtg taaaaaatca
      721 gcctggccca ccaggttttc caccagggag gcaaaattac aatttttaac atcgggccgg
      781 cttcattcca gaccccatcg ttgttggcgg ctttgacccg aaagacatat tctccatgct
      841 caaggttggt aaaagtggcg cgccggtttt tggcatccgt agtaatccag tcgttataca
      901 aaccttcaag tttataagcg tatcggttgt cttccggctg cttgaaattc agcgcactaa
      961 actcaatggt aaagagatta tccgtatgct gtagcaccag cgtttccagc agattgatct
     1021 gagctggaag tgttgattgc gcacggattg gctgaatttg atttcccagc aaaagttcgg
     1081 tcaaaaccac cgatggcggc atcgggttct gagtaatttc ctcggggcga aatgaaataa
     1141 ctccttccgg ggtgccaaaa tacagcctgc cgccttttcc agagcagaca accccgcgat
     1201 ccagcaactc gtgaggtaat ccatcatttt ttccaaaccg caaggtgtgt ccggtgggtg
     1261 ggtcaaatcg aaacagtcca ttgatggtgc caacccaaaa agcacccgaa gcgtcctcca
     1321 caaccgacat aatcgcaaac tctgccagac cagtctcaac tggcacccga ctcaattttc
     1381 cggttgtcgg gtcaatccag cataatccgg cacgagttcc aacccaaatc agcccggttg
     1441 atgcccggta caaacaatca atgaattcat ggttgatact ggctggatta tttggatcgt
     1501 gtagatagcg ggtcatctgc ccggtcgaag gatcaagccg atttaatccg gagccagtgc
     1561 caatccaaat atgtctatcc ggtccttcag ccagggcttc aatcatgtca gaactgaggg
     1621 aacctggatc atctggacgg tgacgatagg cggtaaacac ggctttaggc tgatcagagg
     1681 tggagtcagc cagtgcgtac aggccctgat aggtgccgac ccagacggtc ccgtgcgaat
     1741 cttcaagcaa ggccagaatg gcattgtttc caaggctgtg tggatctttt gggtcacggc
     1801 ggtaattggt aaacaccatc cggtttgact ggcgtcgtcc gagaaaaagc ccgctgctga
     1861 tggtaccgac c
//
LOCUS       DAOQDO010001407         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63745_length_1869_cov_3.272878, whole genome shotgun sequence.
ACCESSION   DAOQDO010001407 DAOQDO010000000
VERSION     DAOQDO010001407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63745_length_1869_cov_3.272878"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1034)
                     /locus_tag="PKZ53_19295"
     CDS             complement(165..1034)
                     /locus_tag="PKZ53_19295"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNJ42642.1"
                     /translation="MDTTHQHLSAQIVEVGRRLYERSYIVAGDGNVSVRTPDGFLLVT
                     PSGKNKGLLTPDMIVKTTLKGEHVAGQLRASSELAMHIAIYEERPDVYAVVHAHPPVG
                     TGFAATGLPLDQALVSEVVLTLGCIPLAEYGTPSTKELPDALRRLCHKHDALLMANHG
                     AVAYGPDLETAFNRMETLEHFARISLVAHLLGGGRPLTPPMIAKLIEVREKGGFMPPE
                     MRCQSCSYSADLPGFHCESSETASRPSAPPSGPSDEKLNETITLTRRELIDLIDRATR
                     LAFEQQRLYQTAR"
BASE COUNT          467 a          438 c          494 g          470 t
ORIGIN      
        1 accattccaa gtgcttccat aggtattttg gattcctcgc tactgtaaga ttctcaagag
       61 aaaaaaatcg ctggtactgc caaaaaagca atccagtttg aaaaaaagat gtgtgtgatg
      121 gcgcgcggtt tgagtgaaat ggaattgaac aactgtcgaa aaagttaccg cgctgtttga
      181 tacaaccttt gttgctcgaa tgcaagtctt gtcgcgcgat caatcaaatc aattaattcg
      241 cggcgtgtca aagtaattgt ttcattaagt ttttcgtctg acggaccaga tggtggtgct
      301 gagggccgtg aagcggtctc tgatgactca cagtgaaacc ccggtaagtc tgccgaatac
      361 gagcacgact ggcagcgcat ttccggtggc ataaacccgc ctttttcccg aacttcaatc
      421 aatttggcaa tcatcggcgg tgtcagtggg cggcctccac caagcaaatg ggccaccagg
      481 ctgatgcgcg caaaatgctc aagcgtttcc atccggttaa aggctgtttc aaggtctggt
      541 ccataggcga cggcgccgtg atttgccatc agcaaggcat catgtttgtg gcacagtcgg
      601 cggagggcat ccggcagctc tttggttgat ggggtgccat attcggccag tggaatgcag
      661 cccagggtga gcactacttc tgagaccagc gcctgatcaa gcggcagtcc ggtggcggca
      721 aagccagtgc caaccggagg atgcgcgtga accacggcat acacatcagg gcgttcttca
      781 taaatcgcaa tgtgcatggc cagttcggag gaggctcgca actggcccgc gacatgttcg
      841 cccttgagcg ttgttttgac gatcatgtct ggggttaaaa gccctttgtt tttgcctgac
      901 ggtgtgacaa gcaaaaatcc atcgggcgtg cgaacgctca cattgccatc tccggcaaca
      961 atatagctgc gttcataaag tcttcgcccg acttcaacaa tctgggcgga caggtgctgg
     1021 tgtgttgtat ccattcggtt gaaatcagtt gtgatggctg aaagctgaat ctgcagtcac
     1081 ttcgggattg gcctcagcag gagggaagtc tctgcacgcc tcatattata caggcatctg
     1141 aaggctggca aggagtccat tcgccaggct tctctatggg ctgaaggcat cgggctgaag
     1201 aaaatgggct gaagaagatg ggttgaaggc attgggctga agaaaacggg ctgaaaggag
     1261 tctgatgccg agccatcata aaatttgcta ttccggacaa aaacaggtac aacaccccag
     1321 ttttctcaaa gccaggaaca gatgcgagca tcggtgccac ccggtcaaca aactgagcac
     1381 gtggttgatc atttgaactt cagtcaacca gagtgatctg tccttgatcg tgagggcaca
     1441 aggtatctgg tgctgatacc agtcagtcgt cagtcgtcag tagttcgcta agtttatctg
     1501 gttaaatcac ttgattgttt cctcatccaa gaactttatt gcaaatgagt cgtccctgct
     1561 tttgagaaag ctctccggct ttagatccct tcacctacgc ctcctgcacc cctttaaaca
     1621 ccatcaggat cgaatcagtt tttcgattcg tcattggctg cacacctgtt gccatcgcaa
     1681 ttttttaatg actggcctgg tactggtgtg gtgtgtggag gtaggtgaca agctgattaa
     1741 aacttttttg gatgggcaac gttgtcgtgt ccaaagcaag tcaaaggcga ccaatgagcc
     1801 attaacatgc tgttcagaaa tatgtggtac caaaggcaaa ctgtgagcac gcaatagttt
     1861 gcagataca
//
LOCUS       DAOQDO010001408         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63803_length_1868_cov_3.037507, whole genome shotgun sequence.
ACCESSION   DAOQDO010001408 DAOQDO010000000
VERSION     DAOQDO010001408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63803_length_1868_cov_3.037507"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1868
                     /gene="alr"
                     /locus_tag="PKZ53_19300"
     CDS             <1..>1868
                     /gene="alr"
                     /locus_tag="PKZ53_19300"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNJ42643.1"
                     /translation="VDDPLASLQKLAGWWRKELRGKVLAITGSNGKTIVKDALHQVLC
                     GMYECSASPGSYNNQLGVPLSLLRFPQEVELALVEAGVSEKNDMPVLERMIRPDFGIL
                     TNIGLAHLSAFGSREAIAQEKMQLFANIAPEGWLLVPANEPLLEPSLSTLRCRIHRVG
                     QKNPDLPFVEQRHPSKDGQLLTLHFPGHSTFEFFVDTPSLEIVTDLEIAACAGFLLAV
                     PEAMIVEALTGYNPGSTRMETWKSPNGVTLINDSCSSDPISVRSALRTLATMSNDRGK
                     RIFVFGGMRELGPLEAVEHAQVGYIAAESNVDTLVLIGSRQLDQTEDAFREKAAGQVL
                     RCRNFEEIKSRLLPSLKWGDTILVKGPRNTDINDIAREITETMAPNRFIVDLEAINEN
                     ITRFQRHVGPNTRILAMIKALAYGSDATRLAKELQKMGIESVGVASADEGVSLRKVGV
                     DIPILVFLCTPDEAEKVVRYALTPIVYSSESITPLAAAAQTQGKTINVHLEVDTGMGR
                     LGVQPEQALEVAQAIAQSGRLHLAGVLTHFACAEEPEKDDFTRLQISRFNTVVAQLKA
                     AGFTRLICHAGATAGAARFPEAHFDMVRIGLGLYGVYPSEAVAQAIDLDLAVSLVS"
BASE COUNT          428 a          515 c          503 g          422 t
ORIGIN      
        1 ggtggatgat ccactggcca gcctccaaaa actggctgga tggtggcgga aggaactccg
       61 aggcaaagtc ctggccatca ccggcagcaa cggcaaaacg attgtgaaag acgcgctgca
      121 tcaggtgctg tgcggaatgt atgaatgttc ggccagccca ggcagctata acaatcagct
      181 tggcgtgccg ctctcgctcc tgcgattccc acaggaagtc gaactggcac tggttgaggc
      241 tggtgtttcc gaaaaaaatg atatgccggt gctcgaacgc atgatccgac ctgattttgg
      301 tattttgacc aacattggtc tggcgcatct gtcggccttt ggttcgcgcg aagcgattgc
      361 tcaggaaaaa atgcaactgt ttgccaatat tgcgccggaa ggctggttgt tggtaccggc
      421 caatgaacct ttgcttgagc cctctctctc aacgcttcgg tgccggatcc atcgggttgg
      481 ccagaaaaac ccggacctgc catttgtcga acagcggcac ccatcaaaag acggccagtt
      541 gttgaccctg catttcccag gtcactcgac gtttgagttt tttgttgaca ccccatcgct
      601 ggaaattgtg accgatcttg aaattgcggc ctgtgccggg tttctgctgg cagtgccaga
      661 ggccatgatt gttgaagcct taaccggcta taaccctggc tcaacccgca tggaaacctg
      721 gaagtccccc aacggggtga cactcatcaa cgattcgtgt agttctgacc caatttccgt
      781 tcgctctgcc ctgcggacgc tggccaccat gagcaacgac cggggcaaac gcatttttgt
      841 cttcggcgga atgcgcgaac tcggaccact tgaagccgtc gaacacgccc aggtcggcta
      901 tattgccgct gaaagtaatg tagacacact ggttttgatt ggaagccgcc aactggatca
      961 gaccgaggat gcctttcgtg aaaaggctgc tggccaggtg ctccgctgcc ggaattttga
     1021 ggaaatcaaa agccggttgc tccccagttt aaaatgggga gataccattc tggtcaaagg
     1081 accacgcaat accgacatca acgacattgc ccgcgaaatc acggaaacca tggcgcccaa
     1141 tcggtttatt gttgatctgg aagccatcaa cgaaaacatc acccgctttc aacgccacgt
     1201 cgggccaaac acccgcatcc tggccatgat taaagcgctc gcctatggca gcgatgcaac
     1261 ccggctggct aaagaactcc aaaaaatggg gattgaatca gttggcgtgg ccagtgccga
     1321 cgaaggcgtg tcgctccgga aagtcggcgt ggatattccg attctggttt tcctctgcac
     1381 accagatgaa gctgaaaaag tcgtgcgcta tgcactgacg ccgattgtgt attcctctga
     1441 aagcattaca ccgctggccg ccgccgcaca aacccagggc aaaaccatca atgtccacct
     1501 cgaagttgac accggcatgg gtcggttggg ggtacaaccc gaacaggcac ttgaagttgc
     1561 tcaggccatt gcccagagcg gaagattgca tctggctggt gttctcacac attttgcctg
     1621 tgccgaagaa cctgaaaaag acgactttac ccgccttcaa atcagtcgtt tcaatacggt
     1681 tgtggcccaa ctcaaagccg ctggctttac ccggttgatt tgtcatgccg gtgcaacggc
     1741 tggcgccgcc cggtttcccg aagctcactt tgacatggtt cggattggcc ttggtttata
     1801 cggcgtctat ccgtcggaag ccgtcgctca ggcaattgac ctggatctgg cggtatcgct
     1861 ggtgagtc
//
LOCUS       DAOQDO010001409         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63807_length_1868_cov_2.670160, whole genome shotgun sequence.
ACCESSION   DAOQDO010001409 DAOQDO010000000
VERSION     DAOQDO010001409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63807_length_1868_cov_2.670160"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKZ53_19305"
     CDS             complement(<1..1208)
                     /locus_tag="PKZ53_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42644.1"
                     /translation="MSRFSLAPLVRHSVVSGFVFFAGLSCLVGYLPVTQAQQSPPTEA
                     PQPPSIQVGLVPGIQLEGELQAEQKQSFTVQFEANRYVRIEVEQQGIDVIVRLFDPAG
                     KQVQEMDSPNGTNGPEHLRYVTVEAGTYRVEVAALEKGARSGKYTIRLGEVRPSRPGD
                     ATVIHYDQLANRVNILLRFELYQELLPVAEQLVTAAAEAYGAESAQYASAILQVGEIN
                     YRFRNLPQAESSYVQALALREKLFGPDHAEVSTVLNNLALLYKAKGEFDKAVGTSQRS
                     IAIVEKVFGPNHLEVAMSYNILGETYRTQNKLAPAETTFQTALSRMQKAETPNKSGEA
                     TILNNLALTIYGKGDYLKAVELLKEVVANHKETSGEKHPLYAIGLLNLAEGYRKIGRF
                     AEMEPLMKQAL"
     gene            complement(1336..>1868)
                     /gene="nth"
                     /locus_tag="PKZ53_19310"
     CDS             complement(1336..>1868)
                     /gene="nth"
                     /locus_tag="PKZ53_19310"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNJ42645.1"
                     /translation="TDERVNLVTPTLFRKFRTAADYAEAELSELEMDIRSTGFFRNKA
                     RHLQGMGQRLVEAFDGKVPKTMAELLTLPGVARKTANVVLGNAFGKAPGVVVDTHVTR
                     LSTRLRLTSEATPEKIERDLMALIPKKHWVMFPHWLIFHGRAVCKARSPQCPACVLAD
                     LCPSVQKPDGQKDKKD"
BASE COUNT          420 a          493 c          487 g          468 t
ORIGIN      
        1 agcgcctgtt tcatcaaggg ttccatctcc gcaaagcgtc caattttgcg atacccttcg
       61 gctaaattca ggagcccgat ggcatacagt ggatgttttt caccggaagt ttctttgtgg
      121 ttggcgacca cctctttcag caattcaact gctttgaggt agtcaccttt gccatagatt
      181 gtcagggcga ggttattgag aatcgtcgct tcacctgatt tgttcggagt ttcagccttt
      241 tgcatccggg acagtgcggt ttgaaacgtg gtttccgccg gcgccaactt gttttgcgtc
      301 cggtaggttt caccaaggat gttgtagctc attgccacct cgagatggtt ggggccgaac
      361 actttttcaa caatggcaat ggatcgttgg gaagtgccaa cagccttatc aaactcccct
      421 ttggctttat acaaaagggc cagattgttg agcaccgtgc tgacttctgc atggtcagga
      481 ccgaacaact tttctcgcaa agcgagtgcc tgcacatatg aagattccgc ctggggaagg
      541 ttgcggaagc gatagttgat ttccccaacc tgcagaatcg ccgacgcata ttgcgccgat
      601 tccgctccat aggcttcggc tgctgccgta accagttgct cagcaaccgg aagcagttcc
      661 tgatacagtt caaacctgag caaaatattg acccgattgg caagctggtc atagtggatc
      721 acggtagcgt caccgggacg agacgggcga acttccccca gtcggatggt atattttccc
      781 gacctggctc ctttttccag agccgcaacc tcaacccggt aggttccagc ttcaactgtg
      841 acgtaccgta ggtgctctgg tccgttggtt ccattgggac tgtccatttc ctgaacctgt
      901 ttcccagccg ggtcaaacag gcgcacgatg acatcaatcc cttgttgctc aacctcgatg
      961 cgcacatacc ggttggcttc aaattgcacg gtaaaactct gtttctgttc ggcttgcagt
     1021 tcaccttcaa gttgaatgcc tggcaccagg ccaacctgga tggatggcgg ttggggagcc
     1081 tcagtcggtg gcgattgttg agcctgggtt acgggtaagt agccgacaag gcaggacaga
     1141 cccgcgaaaa aaacgaaacc actcaccacc gaatggcgaa ccaggggagc caaagaaaat
     1201 cgagacatat gtttggtttt taactttccg ggaagctcaa tatcaataga ttgagcctga
     1261 tgaccccaat acttgagaat aaactgcatc ttcagcaaag ccaggtgcct ctgtctttgc
     1321 gtccgtgtgg tcacttcagt cctttttgtc cttttgaccg tcaggctttt ggactgatgg
     1381 acacaaatca gccagcacac aggccgggca ttgcggcgac cgggccttgc aaaccgcccg
     1441 cccatgaaaa atcagccagt gtggaaacat cacccagtgt tttttcggaa taagtgccat
     1501 cagatctcgc tcgatttttt caggcgtcgc ttctgaggtt agtcgcaatc gagtggaaag
     1561 ccgggttacg tgggtgtcta ccacaacgcc aggcgctttg ccaaacgcat tgcccaggac
     1621 gacattcgcc gttttgcgag ccacgccggg cagggtgagg agttcagcca tggttttcgg
     1681 gactttcccg tcaaaggctt cgaccagccg ttgccccatg ccttgtaagt gacgggcttt
     1741 attgcgaaaa aagccggttg aacgaatatc catttccagt tcggaaagct cagcttcggc
     1801 ataatcggcg gcagtgcgga acttacgaaa cagcgtcgga gtgaccagat tgacccgttc
     1861 atcggtac
//
LOCUS       DAOQDO010001410         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63814_length_1868_cov_2.478764, whole genome shotgun sequence.
ACCESSION   DAOQDO010001410 DAOQDO010000000
VERSION     DAOQDO010001410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63814_length_1868_cov_2.478764"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1045)
                     /locus_tag="PKZ53_19315"
     CDS             complement(59..1045)
                     /locus_tag="PKZ53_19315"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNJ42646.1"
                     /translation="MRLFTIFFVSCLVTLISICPVRSQQEGSAPQFSPYFRVYTGDGK
                     PVAMADISQAMAGVEVVFVGETHNDPMAHSLELAILKRAHEAASSSPGDAGGRNAVLS
                     MEMFERDTQPVLDEYLTDIISERHFLESSRPWTNYKNDYRPLVEFAREQKLTVLAANA
                     PKRYANLVSRQGRNALSTALPRAAQWLPPVPYGFPSPAYKAKFEALFAQPEKPLPPSL
                     SSKGFFQHGMGNLQYTLDAQSLWDATMAFSMAEELLRHPNSLIVHVVGKFHCEGRLGI
                     PDHLLRYRPGTKFVVVTVEPADDITTLSEKHKGLGDFVILTDSKLPRSYEDK"
     gene            complement(1173..>1868)
                     /locus_tag="PKZ53_19320"
     CDS             complement(1173..>1868)
                     /locus_tag="PKZ53_19320"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ42647.1"
                     /translation="LQAKLLRVLEEGTFRRVGGLKDVSLDTRVIAASNRDLKSECQEG
                     KFRLDLYYRLSVIQIDVPALRERGDDGLILANHFISQFKQQLRKKTRGLSSEVEDIFR
                     KYSWPGNVRELRNVIERVMILEDGDLITTKYLPRDLILPEYHRPIVTAPLASPPAQQV
                     TSAPPPVESKPPSGSQHMFQLPAEGISIDEVEMSLVQQAMQLSGGNQTNAAKLLRISR
                     DQLRYRLKKLEGN"
BASE COUNT          462 a          480 c          469 g          457 t
ORIGIN      
        1 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc ctatgaattt
       61 atttatcctc ataactccgg ggcaattttg aatccgtcaa aatcacaaaa tcccccagcc
      121 ctttgtgttt ctcgctcaaa gtggtgatgt catcagccgg ctcaacggta acaaccacaa
      181 atttggtgcc aggccgatag cgaagcagat ggtcaggtat gcccaaccga ccttcacagt
      241 gaaattttcc aaccacatga acaatcaaag aattcggatg gcgtaataat tcttccgcca
      301 tagagaaggc catggtggca tcccagagcg actgggcatc cagtgtatat tgcaaattcc
      361 ccattccgtg ctgaaaaaag cctttgcttg acagactcgg tgggagcggt ttttcaggtt
      421 gagcaaagag tgcctcaaat ttggctttgt acgccgggct tgggaagcca tatggcaccg
      481 gcggcaacca ttgagcagcg cggggtaagg cggtactgag cgcattgcgc ccctgacgtg
      541 aaaccagatt ggcatagcgt tttggagcat tggcggccag gaccgtgagt ttttgttcac
      601 gcgcaaattc caccagtgga cgatagtcgt ttttatagtt tgtccaggga cgactacttt
      661 ccaggaaatg gcgctctgag atgatatcgg tcagatattc gtctaacacc ggctgggtat
      721 cccgttcaaa catctccatt gataacacgg catttcgtcc accagcatcg cctgggctgg
      781 atgaagcggc ttcatgtgcc cgttttaaaa tggccagttc cagactatgg gccatcgggt
      841 cgttgtgtgt ttctccgaca aagacgactt caaccccagc catggcctgg gagatatcag
      901 ccattgccac cggttttcca tctccggtat agacgcggaa atagggtgaa aactgagggg
      961 cgcttccctc ttgctgactg cgaaccgggc aaattgagat caacgtgacc agacaggata
     1021 caaagaaaat tgtaaataat cgcatagttt tttcattgga attcagatca gtaacagagt
     1081 gacaagatga caagatgacc gaatgacagg gtgatttctt tcacccctta tccttcatcc
     1141 ctcatcgctt cgattgccct cagccctgga ttttaattgc cttctaattt cttgaggcga
     1201 tagcgaagct ggtctcgcga aatacgcaac agtttggctg cgttggtttg attgccaccg
     1261 ctcagttgca ttgcctgctg gacgagcgac atttcgacct cgtcaatcga aatcccttct
     1321 gctggaagct gaaacatgtg ctggctccca gatggtggtt tggattcaac cggtggtggg
     1381 gctgacgtta cctgttgggc aggtgggctg gccagcgggg ccgtcacaat tggacggtgg
     1441 tattccggca aaatcaggtc acgcggcaaa tatttggtgg taatcaaatc accatcttcc
     1501 aaaatcatga cccgttcaat gacgtttcgg agttcacgaa cattccccgg ccacgaatac
     1561 tttcgaaaga tgtcttcaac ctctgaactc aggccacggg tttttttgcg caattgctgt
     1621 ttaaattgac tgataaagtg attggccaga atcaagccat cgtcaccacg ttcgcgcagg
     1681 gccggaacat caatttgaat caccgagagc cggtaataga gatcaagccg gaattttcct
     1741 tcctggcact ctgacttgag atcccggttg gaagcggcaa tgacccgtgt atccagggac
     1801 acatctttta agccacccac ccggcgaaag gtcccttcct ctaacacacg aaggagtttg
     1861 gcctgcag
//
LOCUS       DAOQDO010001411         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_63951_length_1866_cov_2.308669, whole genome shotgun sequence.
ACCESSION   DAOQDO010001411 DAOQDO010000000
VERSION     DAOQDO010001411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63951_length_1866_cov_2.308669"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..>1866
                     /locus_tag="PKZ53_19325"
     CDS             357..>1866
                     /locus_tag="PKZ53_19325"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNJ42648.1"
                     /translation="MMNDKCHFSRWFTFITVIGMCVFQPGFGTWIANGKPLLKQSANA
                     AQTTFSTYLGGNSSESIVTTLADTGGNSYVIGLTSSTDFPTLNAIQSTLTLPTVPGAT
                     VTKFDQSGRLVYSTYIAGRELLTIHDAKVDAGGSLHLVGSTTAKNLPTAKAFQPENGG
                     RRDLFAAKLDPTGSLVFLTYLGGRSNEEFKAFELDFAGNMFIAGTTLSANFPQKRAFQ
                     KKTDDPNRDGEIDVFVTKLDQAGKLVFSTPLGGTGSDTEALVAMKVDRSGNVVLAGTT
                     DSGSFPVKKAFQPKRRGTTDVFVTKLDARGKAIYSTYLGGGGPDLVHGIALDQSDNVV
                     VFGITNAVDYPTVNAFQSAYSGAEDIFLTKLNVIGGVVFSTLLGGSSNEHIGLPSTYT
                     NGLSIDSQGFIYVNGYTLSTDFGTRNAVQSVSTGGGDHFAARLSPTGEMIFCTYLGGR
                     AREELGRGFVDSGQNLYVIGTTWSGNYPVANAFQPEHGGAADDGGQSGTDSAQ"
BASE COUNT          442 a          493 c          475 g          456 t
ORIGIN      
        1 caaagcccct ggcaagttgc gttagtgttg tttctatcgg gtgagaacct atctccgaag
       61 gcaggcccaa ccggcctgcc ttctgttttc ttgaatcagg cttgaatcca accctggtcg
      121 aaaccgtggg cttcgcgcca cggctattgc acaatgaccc tccgggccta aagcccctat
      181 cctccattcc cttgttccct cacctctggg cgtggtgtaa tgcctcagac taaccaacca
      241 tgcacacgat agaatcattc tcctctgtca ggtacttttc actcctggca gatgcacacg
      301 aactcagatc tttattaaac actctattgc aaccagatta taaggagcac acttttatga
      361 tgaatgataa atgccacttt tcacgatggt tcacctttat tacagtaatc ggaatgtgtg
      421 tctttcagcc tgggttcggc acgtggatcg cgaatggaaa accactcctg aagcaatctg
      481 cgaatgccgc ccaaacaacg ttttcaacct atcttggcgg aaatagcagc gaatcaattg
      541 tgacgacgct ggctgatacg ggaggaaaca gctacgtcat cgggttaacc agttcgactg
      601 attttccaac gctcaatgcg attcaatcca cgttgacgct cccaactgtg ccgggagcga
      661 cggtgacgaa atttgatcag tcaggacggc tggtgtattc gacctacatt gccggacgcg
      721 aactcctgac gattcacgac gccaaggtgg atgccggagg gtcgctccat ctggttggct
      781 cgaccaccgc gaagaattta cccacggcca aagccttcca accggaaaat ggcggacggc
      841 gtgatttatt tgccgccaaa cttgacccga ccggaagcct cgtgtttttg acctatctcg
      901 gaggccgctc caacgaagaa ttcaaagcct tcgaactcga ctttgccggc aacatgttta
      961 ttgcgggcac aacgctttcg gcgaacttcc cgcaaaagcg cgctttccag aaaaaaacgg
     1021 atgatccaaa ccgtgacgga gagattgacg tgtttgtgac caagctcgat caggcgggga
     1081 aactcgtctt ttcaacgccg ctcggtggaa cagggagcga taccgaagcc ctggttgcca
     1141 tgaaagttga tcgttcggga aatgttgttc tggctggcac gacggattcc ggttcattcc
     1201 cagtgaaaaa agccttccag cccaaacgcc ggggaacgac ggatgtcttt gttacaaagc
     1261 tcgatgcgcg tggaaaagcg atttattcaa cctatcttgg cggcggcggg ccagatctgg
     1321 tccacggtat tgcccttgat caatcagaca atgtcgtggt tttcgggatt accaacgcgg
     1381 ttgattatcc aactgtcaat gcgtttcaat cagcctatag cggtgcggaa gatatttttt
     1441 tgacgaaatt gaatgtgatc ggtggcgtgg ttttttcgac gcttctgggt ggcagcagca
     1501 acgagcacat tgggttgccg tctacataca ccaacgggtt aagtattgac tcacaaggat
     1561 ttatctatgt gaacggctac acgctttcta ccgactttgg cacacgcaat gccgttcaat
     1621 cggtcagtac tggtggcgga gaccattttg ccgcccgact ttcgcccacc ggagagatga
     1681 tcttttgcac ctatctgggc ggacgcgccc gcgaagaact cggacgtgga tttgttgatt
     1741 ccggtcaaaa tttgtatgtc atcggcacga cgtggtcagg aaattatcca gtggccaatg
     1801 cctttcaacc cgaacatggc ggcgcggctg atgatggcgg ccaaagcggg acagattctg
     1861 ctcagc
//
LOCUS       DAOQDO010001412         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64033_length_1865_cov_2.254696, whole genome shotgun sequence.
ACCESSION   DAOQDO010001412 DAOQDO010000000
VERSION     DAOQDO010001412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64033_length_1865_cov_2.254696"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="PKZ53_19330"
     CDS             <1..1602
                     /locus_tag="PKZ53_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ42649.1"
                     /translation="TTDAGQSWTPISSDLTRNDKSKQKWSGGPITGDNTGVEVYCTIF
                     AVAESPKQKDLLWVGSDDGLVHVSRDGGKNWTNVTGKMPGFPEWGTVKCIEASPHDAG
                     TAYVVVEAHRLDNMQPYLYKTSDFGQSWKRLTDKLPQDVYLHAVREDPKRKGMLYVGT
                     ERGIVYSTTDGADWKPLKFNFPTVAVCDLAVKDNSLVVATIGRSLWILDDLTPIREMT
                     DKVTGQDVFLFPTSDVHRWRYHYSTPDKYAGDNPAAGAVIFYHLKQKAKELKLEILDD
                     KGTVIRTLSSVPEPKEADEAEYSEGDEDEPKDVLSTEPGLQSVEWDLAHTGAKLIKGA
                     KVDAGTPKVGPTAVPGTYTLRLLVDGKTLTTTLVIKPDPRVQLTQAQLVEQLQFALKI
                     RDDISTLTETVEQLRSLKQQLDARCQLLKDTEKAAELVKAAKALTEKLDALEAKLHNP
                     KAKVTYDILAQKGGAKLYSQLSPLLEWVKDSDGVPTQGMREVYAGLSVELAQYRSEMK
                     SLVDTDLAAINKMAKDLDLPYIGVK"
BASE COUNT          484 a          476 c          488 g          417 t
ORIGIN      
        1 accaccgatg ccggccaaag ctggacgccg atcagttcag atttaacccg caatgacaag
       61 agcaaacaga aatggtcggg cggaccgatc actggcgaca ataccggcgt tgaggtttac
      121 tgcacgattt ttgccgtggc ggagtctccg aaacaaaaag atctgctctg ggtgggttcg
      181 gatgatgggc tggttcacgt gtcacgtgac ggcggcaaaa actggaccaa tgtcactggg
      241 aaaatgccgg gattccctga gtggggcacc gtgaaatgca ttgaagcctc tccgcacgat
      301 gcgggcaccg cctatgtggt cgtcgaagcc caccggctcg acaatatgca gccctatctg
      361 tacaaaactt cagattttgg ccagtcctgg aagcgactca ctgacaaact gccccaggat
      421 gtatacctgc atgcggttcg cgaagatccc aaacgtaaag ggatgctgta tgtcggaacc
      481 gaacgcggta tcgtctattc aaccaccgac ggcgctgact ggaaaccact gaaattcaat
      541 ttcccaaccg tggcggtatg tgatctggcc gttaaagata acagcctggt tgtagccacc
      601 attggacgtt cgctttggat tctggatgat ctgacaccga ttcgtgaaat gaccgacaag
      661 gtgacaggtc aggatgtatt tctcttccca acatctgatg ttcaccgctg gcggtatcac
      721 tactcaactc cagacaaata tgcgggggac aatccggcag ccggggcggt gatcttctac
      781 catttgaagc aaaaagcgaa agaactcaaa ctggaaattc tggatgacaa aggaacggta
      841 attcgcactt tgagcagtgt tccagaaccc aaagaagccg acgaagctga atattccgaa
      901 ggcgatgaag acgagcccaa agacgtgctt tcaacggaac cgggcttgca aagcgtggaa
      961 tgggatctcg cccacaccgg cgccaaattg atcaaaggtg ccaaggttga cgcgggaaca
     1021 ccgaaggtcg ggccaactgc cgtgcctggc acctacacgc tccggcttct ggttgatgga
     1081 aagaccctga caacgacgct ggtgatcaag ccggatccac gggttcaact gacacaggca
     1141 caactcgttg aacagctcca gtttgcactt aaaattcggg atgatatttc aacactgacc
     1201 gaaaccgtcg agcagttgcg ctctcttaag caacaacttg atgcccgttg ccagcttttg
     1261 aaagacactg aaaaagccgc tgaactggtt aaggctgcca aagccctgac ggaaaaactg
     1321 gatgcactcg aagccaaact tcataacccc aaggccaaag ttacctatga cattctggcg
     1381 caaaaaggcg gagcaaaatt gtattctcag ttgagtccat tgcttgaatg ggtcaaagac
     1441 agcgacggcg taccgacgca aggaatgcgt gaagtctacg cgggtctttc agtcgaactc
     1501 gcccagtacc ggagcgaaat gaaatcgctg gttgataccg acctggccgc tatcaacaaa
     1561 atggcaaagg atctggattt gccctatatc ggggtaaagt aaatccaggg ttcagggttc
     1621 agggttcagg gtttttaagc aggcgcagtt cacagcagca gcgcccttcc cccctgtcgc
     1681 cttgtcattc tgacattttt ccagggttcc gggtttttga aaccggcgcg ttcccttcga
     1741 aaggaatgcg ccggttgttc tttttacttt ttcgactttt gttttcgccg gggtttcttt
     1801 gcttcaggat aggaaatatc acgaagcaat cgtctgattt tttcaatctg caggtcctgg
     1861 tcatc
//
LOCUS       DAOQDO010001413         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64088_length_1864_cov_2.485351, whole genome shotgun sequence.
ACCESSION   DAOQDO010001413 DAOQDO010000000
VERSION     DAOQDO010001413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64088_length_1864_cov_2.485351"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PKZ53_19335"
     CDS             complement(<1..990)
                     /locus_tag="PKZ53_19335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNJ42650.1"
                     /translation="MSKVAARPTPQTTTCPRCGSLQPLSQPCPHCQTSPKLAPQPVQS
                     KLMANALIILAGVLFLCVTALLISKPGIQFLGFYLAAGIGVCVGNVWVRHRRESGQTP
                     KLDFQDPYLIAYLRGGAPEALRIATVELLTRKLLVEKVTVDSSDIYTLIQANPAAKTK
                     PQTQIEASLLVWFQESNGPTSIFDNTQLTRACLYLNDWLEQLGLLPNQSVRRARRIQF
                     GAAFSVLIGLAGLKIVYALATGHTNILFLVLLAIGFSMVLVKTSFPRRTGLGDQVLKD
                     LKKLYQSARNSGRNQPGNKNEVMLASVFGLAALPPTSLVLAQKFYPQAANPTST"
     gene            1272..>1864
                     /locus_tag="PKZ53_19340"
     CDS             1272..>1864
                     /locus_tag="PKZ53_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ42651.1"
                     /translation="MGVKERRQREKESLRQEILDAARELFAAEGYDNVSMRKIAEKIE
                     YTPTTIYLYFQDKAELLYGICQEMFGELIQSYSKLGELEPDPVSALKRGLRIYIDFGL
                     SHPNHYKVVFSGMNHNCDFEKRFPFEQSLGHEAFSYLVGIVQACIDQKVFRPVDVATA
                     SQTIWAALHGLVVLLIDHQDFPWVDREQLINHLIDTLV"
BASE COUNT          464 a          422 c          505 g          473 t
ORIGIN      
        1 ggtactggtt ggattggccg cctgggggta gaatttttga gccagaacca gactggttgg
       61 cgggagggct gccagcccaa aaacggacgc cagcatgacc tcattcttat tcccgggctg
      121 gtttctgcca gaattgcggg cagattgata cagttttttc aggtctttca gcacctggtc
      181 acctaaccca gttcgtcttg gaaaggaagt tttgaccagc accatcgaaa acccaatggc
      241 gagcagcacc agaaacaaaa tattggtgtg acccgtggcc agggcataaa caatcttgag
      301 accggccaaa ccaatcagga ctgaaaatgc tgctccaaat tgaattcggc gtgcccggcg
      361 caccgattga ttgggcagta acccaagctg ctccagccag tcatttaaat agagacaggc
      421 tctggtaagt tgagtgttgt caaaaattga agttgggccg ttggattcct ggaaccagac
      481 caggagagag gcttcgatct gagtctgagg tttggtcttt gcggcaggat tggcctggat
      541 caaagtgtaa atatctgatg aatcaaccgt cactttctca accaggagtt ttcgggttaa
      601 cagctcaacc gtggcgatgc gcagggcttc tggcgcccca ccacgcagat aagcaattaa
      661 atatgggtct tgaaaatcca gttttggggt ttggccgctt tcccgacggt ggcgtaccca
      721 gacattcccc acacagacgc caatgcctgc tgccagataa aacccgagaa actggatgcc
      781 aggtttggaa atcagcagtg ccgtgacaca gagaaacaga actccggcca gaatgatcag
      841 ggcattggcc atcaatttcg attgaacagg ttgaggagct aatttgggtg atgtctgaca
      901 gtgtggacag ggttgggaaa ggggttgaag tgaaccgcaa cgtgggcagg ttgtggtttg
      961 tggtgtcggt ctggcggcaa cttttgacat aagaacctcc ttggttggta aaggttgttg
     1021 ccggttcaag ggaacgcttt cagaggcgtt tggtgtggta cggtgcccac gtaaacggga
     1081 tgttacatct cagagcgagg tagcgaagtg tttcagcttt gattcccaat tttgatctgt
     1141 caaccctgga ccctgtattt tcactacttc gctcattttg gagttgacac acgggtcaaa
     1201 aacagtatac ttaacgctgt taagtaaatt ttctttgttt ttcgaaatga tgcgaggaag
     1261 ttgagttcga tatgggtgtg aaagaacgtc ggcaacgtga aaaagaatct ctgcgccagg
     1321 aaatcctgga cgccgcccgc gaactatttg ccgctgaagg ctatgacaat gtctccatgc
     1381 gcaagattgc cgaaaagatc gaatacaccc caaccacaat ctacctctat tttcaggata
     1441 aagccgaact gctctatggc atttgtcagg aaatgtttgg cgaactgatt cagtcctaca
     1501 gcaagctggg tgaattggaa ccggatccgg tttcggctct gaaacgggga ttgcgcattt
     1561 atattgattt tggattgtcg catccgaatc actacaaggt ggttttcagc gggatgaacc
     1621 acaattgtga ttttgagaaa cgcttcccgt ttgaacagtc ccttgggcac gaagctttca
     1681 gctatctggt cggaattgtt caggcgtgta ttgaccagaa ggttttccgc ccggttgatg
     1741 tcgccaccgc cagtcaaacg atctgggcgg cactccacgg actggtggtg ctgttaattg
     1801 accatcagga tttcccctgg gtggatcgcg aacaactcat caaccatttg attgatactc
     1861 tggt
//
LOCUS       DAOQDO010001414         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64208_length_1862_cov_2.920310, whole genome shotgun sequence.
ACCESSION   DAOQDO010001414 DAOQDO010000000
VERSION     DAOQDO010001414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64208_length_1862_cov_2.920310"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PKZ53_19345"
     CDS             complement(<1..1387)
                     /locus_tag="PKZ53_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNJ42652.1"
                     /translation="MTQEHKNPSPLQQTAEAKRLEESRNRTAHWKRWGPYVSERAWGT
                     VREDYSPYGSAWDYFPHDHARSKAYRWGEDGLAGISDRRQFICFALSLWNENDPILKE
                     RLFGLTGPEGNHGEDVKEYYFYLDSTPTHSYMKWLYKYPQAAFPYWQLIQENRNRSRA
                     EPEFELLDTGIFNENRYFDVTVEYAKASPDDLHVRISVINRGPDPAPIHVLPVLWFRN
                     TWTWKSNVEKPKLYRIDPVDTTSVVELNHPYYGKRWLYCEDSPELLFTENDTNVQRLY
                     NAQNPTPYVKDGINEYVVNHNAGAVNPAQFGTKVSPHYKMVIQPGETKTISLRLTDHQ
                     VNRYPLGAEHDHVLAARKQEADEFYSHLISPHLSVDAQNVQRQAFAGMLWSKQFFYYV
                     IEEWLNGDQTQPKPPAERRKGRNKNWAHLYNEDIISMPDKWEYPWYAAWDLAFHCVPL
                     AQIDPDFAKEQL"
     gene            1748..1822
                     /locus_tag="PKZ53_19350"
     tRNA            1748..1822
                     /locus_tag="PKZ53_19350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1780..1782,aa:Val,seq:tac)
BASE COUNT          413 a          444 c          517 g          488 t
ORIGIN      
        1 tgagctgttc cttggcaaaa tccggatcaa tctgggccag cggtacgcag tggaaagcca
       61 gatcccaggc ggcataccac ggatattccc acttgtcagg catcgaaatg atgtcttcgt
      121 tatagagatg cgcccagttt ttgttgcgtc ctttgcgacg ttcggctggc ggcttgggct
      181 gggtctgatc gccgttgagc cattcttcaa tcacatagta gaaaaactgc ttgctccaga
      241 gcatgccggc aaatgcctga cgctgcacgt tctgggcgtc aactgaaagg tgtggcgaaa
      301 tcaggtggct gtaaaactcg tcggcttcct gtttgcgagc ggccaggaca tgatcgtgtt
      361 cggcgccaag tggatagcgg ttgacctggt gatcggtcag gcgaaggctg atggttttgg
      421 tttcgcctgg ttggatgacc attttgtaat ggggtgaaac tttggtgcca aattgagctg
      481 gatttaccgc cccggcgttg tgattgacga catattcatt gatgccgtct ttgacatatg
      541 gggtcggatt ctgggcgtta tagagccgct ggacgttggt atcgttttcg gtaaagagca
      601 attccgggct gtcctcgcag tacaaccagc gctttccata gtaggggtga ttgagctcca
      661 cgacactcgt cgtatcaacc gggtcaatgc gatagagttt tggcttttcg acatttgatt
      721 tccaggtcca ggtattgcga aaccatagca ctggcaggac atgaatgggt gccggatccg
      781 gcccccgatt gatgactgaa atccgcacgt gcagatcatc tggtgaggct ttggcatatt
      841 cgaccgtgac atcaaaatag cgattttcat tgaaaatgcc cgtatcaagc agctcaaatt
      901 caggttcggc ccggctgcgg ttgcggtttt cctggatcaa ttgccagtag ggaaatgccg
      961 cctgcggata cttatacagc catttcatgt atgagtgcgt tggcgttgaa tcgagataaa
     1021 aatagtattc cttgacgtct tcgccgtgat tgccttccgg cccggtcaaa ccaaagaggc
     1081 gttctttcaa gatgggatca ttttcattcc acaaactgag cgcaaaacaa ataaattggc
     1141 ggcggtcact aatccctgcc agcccatctt caccccagcg atatgctttg gagcgggcat
     1201 ggtcgtgcgg gaaatagtcc caggccgaac cgtatgggct atagtcttca cggacggtgc
     1261 cccaggcccg ttcactgaca taggggcccc agcgtttcca gtgtgccgtg cggttgcgtg
     1321 attcctccag gcgtttggcc tcggcggtct gttggagcgg agaggggttt ttgtgttctt
     1381 gggtcatgat gagcctaccg taggttgagt gagactgttg tcgaacgatt cacacccctt
     1441 ttcggtcagg acacacacgt tggagtctca ggccgtgggc aaaaaggcgt gtaaagcggt
     1501 gatgttcgat gttttcggaa gtggaaatgc actgaaaagg tagtgaagtg atgtgcattc
     1561 cttaacacaa aatccaggtt cacgccaatt tcatttcaag aaattctgcc tccagacaga
     1621 ctttaccagc gagtcgtccc ctttttttca attccggcaa attttcaaaa aaaactgttg
     1681 acagcctgca ccctcgtggc tatattgctc gctcgctttg aaaacccggc aaaacggttg
     1741 cttgtagggg cggttagctc agcggtagag caccggtctt acaaaccggg ggtcactggt
     1801 tcgaaaccag taccgcccac cagattcaaa gtgagatgtt cgtttctcaa aagtttatgg
     1861 ag
//
LOCUS       DAOQDO010001415         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64346_length_1860_cov_3.445983, whole genome shotgun sequence.
ACCESSION   DAOQDO010001415 DAOQDO010000000
VERSION     DAOQDO010001415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64346_length_1860_cov_3.445983"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKZ53_19355"
     CDS             complement(<1..343)
                     /locus_tag="PKZ53_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ42653.1"
                     /translation="MAEFSREEVIQKVANGEGLERADLRDLKLAQAQLEGANLRRADL
                     EGVDFEESVLRKANLASANLRDAFLARANLEGANLRGADLESANLEGANLSDADLSRT
                     NLEGANLENANL"
     gene            complement(729..>1860)
                     /locus_tag="PKZ53_19360"
     CDS             complement(729..>1860)
                     /locus_tag="PKZ53_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42654.1"
                     /translation="LPAISDVAPTLVMDGAFVAEALHAEPVTGAESTAEFFGGAVETT
                     AGPGLVTDEEVTAPTAGSFFDDELGDFDDAFDSLMAGEAPAHADGESTGVEVDQGEIR
                     ELFANIAANYIKPVKNFIFELRSGAARKEWLDICQPALMSLSKSANGMGLLEVSDAVG
                     NFDALLIEARASFEPSITGDLRERVLAYYETLVEMLPQTFTLGEESAQSEGMIINSLL
                     KQIPDVGKVTIDKLYRAGLTTLESYFMGSKEDIAVASGLPIWLAERICEKFRQYQHDL
                     ETSPVDAGNSAHRARLEHLTQDLKEFHDQYEKATAAEWTNPAAAEDRRYYRQARQDTQ
                     LQINIVLAELQAIDLVKEIQRLSFEKRIQRLQEFLQALANEM"
BASE COUNT          459 a          525 c          428 g          448 t
ORIGIN      
        1 ataaattcgc attttccaga ttggcgcctt ccaggtttgt tcgtgacaga tcggcgtccg
       61 acaggttggc tccctccagg ttggcacttt ccaggtcagc cccacgcagg ttggcccctt
      121 ccaggttggc ccgtgctaaa aaggcgtccc gcaagttggc actcgcaagg ttggccttgc
      181 gcaacaccga ctcttcaaaa tcgacacctt ccaggtctgc ccggcgtaaa ttcgcccctt
      241 ccagttgggc ctgcgcaagc tttaaatcac gcaaatcggc acgctccagg ccctctccat
      301 tcgctacctt ctgaattact tcttcacggg aaaattcagc cataaagact ctccctctta
      361 attgttgtta gcttcccagc gcagagaata gttgactctt tcgttgtgca tgtgaagatg
      421 gcgccgcagg agcaagggcg tcacccggaa aagtgcatta aatataaagc cggcaatgtt
      481 tccaccacca gactcaacct tgtcggacag gtttaatcga ttcgtgatga gaaatgaatt
      541 ctgaatgatg aaaaggcgaa ccattgatga tttaacgagc tgtccttttc atcattcata
      601 attcacaatt catacttcct ggaactggat ttcagaagac caatgaattc cggcacctga
      661 aaaaaataac tcctgaccaa ccaaaacaag tatgaaccag cagggtctgc tgattcatat
      721 cccaatcact acatttcgtt tgccagggcc tggagaaatt cctgaagccg ctgaattcgc
      781 ttttcaaacg aaagtcgctg aatttccttc accaggtcaa tcgcctgcag ttcagccaac
      841 acaatattga tctgaagctg ggtatcctgc cgggcctgac ggtaataacg tcgatcctca
      901 gccgccgccg ggtttgtcca ttcggcagca gtggcttttt catactggtc gtgaaattct
      961 tttaaatcct gggtaaggtg ttccaatctg gcacggtggg cgctgtttcc agcatcaacc
     1021 ggactggttt caaggtcatg ctggtactgc cggaactttt cacaaatccg ttcagccagc
     1081 caaatgggga ggccagaggc aaccgcaata tcttctttgg agcccataaa atagctctca
     1141 agtgtggtca acccggcccg atagagctta tcaatggtga ccttcccgac atctgggatt
     1201 tgcttgagca gcgagttgat aatcatgcct tcagactggg cactttcctc acccagggtg
     1261 aaggtctggg gcagcatttc aaccagggtt tcataatagg ccaggacccg ctcgcgcaaa
     1321 tcaccggtga ttgaaggctc aaacgaggct ctggcttcaa tgagcaacgc atcaaagttc
     1381 cccacggcgt cactgacctc aagtaacccc attccattgg ctgatttgga gagactcatc
     1441 aacgccggct ggcagatatc aagccattct ttgcgtgccg cacctgatcg gagttcaaaa
     1501 ataaagttct tgacgggctt gatgtaattg gcagcaatgt tggcaaacag ttcacgaatt
     1561 tcgccctggt caacctcaac accagttgat tccccatcgg catgagccgg cgcttcaccg
     1621 gccatcaacg aatcaaacgc atcgtcaaaa tcaccgagtt catcatcaaa gaaggaccca
     1681 gccgttggcg cagttacttc ttcatccgtc acaagtccag gcccagccgt tgtttccacg
     1741 gcgccgccaa agaattcagc cgttgattcc gccccggtca caggttcggc atgcagtgcc
     1801 tcagcgacaa aagccccatc catgaccagc gtcggcgcca catcactgat cgccggcaat
//
LOCUS       DAOQDO010001416         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64405_length_1859_cov_3.652439, whole genome shotgun sequence.
ACCESSION   DAOQDO010001416 DAOQDO010000000
VERSION     DAOQDO010001416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64405_length_1859_cov_3.652439"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKZ53_19365"
     CDS             complement(<1..338)
                     /locus_tag="PKZ53_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ42655.1"
                     /translation="MQKRLSIVIPIYNEIKTLRLLIERVQAVNIPLEKELVCVDDGST
                     DGTRDLLAQLTREYPNIRVFLQPKNCGKGAALHEGFRQATGDFVIVQDADLEYNPEDY
                     HKVLAPLIEG"
     gene            complement(434..>1859)
                     /locus_tag="PKZ53_19370"
     CDS             complement(434..>1859)
                     /locus_tag="PKZ53_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNJ42656.1"
                     /translation="VARGMGKCCVAGCGSLEIDYDARQFKVGDRVIKEGEYITLDGST
                     GRVIEGQVPLIEPEITGDFREFMSWADEFRTLNVRTNADTPHDSHVARGFGAEGIGLC
                     RTEHMFFEGNRIDMVRQMIMVASEYKRLESERSLIHQEIAKETAKAGGGKEKLAELKK
                     QQAKLDKKIVKPKQLFLGALAALLPMQREDFIGIFTAMNGLPVTIRLLDPPLHEFLPH
                     SVEDMKLLAKKVGLTPKVVEAKIEALREANPMLGHRGCRLGLVYPEITEMQARAIFEA
                     AAHVQQHDVKVLPEVMIPLVGDVQEFVRQEHIVRRVANEVMDATGEKFDYLVGTMIEL
                     PRAALTADRIATAAEFFSFGTNDLTQTTFGLSRDDSGTFLPFYVENKILKDDPFQVLD
                     QEGVGQLVEIGVKKGRNARPGIKIGICGEHGGEPSSVNFCHRVGMNYVSCSPYRVPIA
                     RLAAAQAALAEKSGESQQAISRTA"
BASE COUNT          409 a          513 c          471 g          466 t
ORIGIN      
        1 tcgccttcga tgagcggtgc cagcacttta tggtaatcct cagggttata ttccaggtcc
       61 gcatcctgaa caatcacaaa atcgccggtt gcctgtcgaa atccttcgtg cagcgccgcc
      121 ccttttccac aattttttgg ctgaagaaac acccggatat tgggatattc gcgggttaat
      181 tgagccagca aatcacgcgt gccatcggtt gaaccatcat caacacatac cagttctttt
      241 tcgagcggaa tgtttacggc ctgtacccgc tcgataagca accgcaatgt tttgatttca
      301 ttgtagatag gaatgacaat tgagaggcgt ttttgcatag gaggatcaga tacgaggaaa
      361 gaagttttag agtgcggcgg cttgatggcg ctctgtgaaa gcggcgtcaa gccgccgcac
      421 tccaaaagtt ttatcaggcg gtgcgtgaaa tcgcctgttg cgattcaccg gatttttcag
      481 ccagagcagc ctgagcggct gccagacggg caatcggcac ccggtatggt gagcaactga
      541 cgtaattcat cccgacccgg tggcagaaat tgacgcttga cggttcgccg ccatgttcgc
      601 cgcaaatccc gatcttgatc ccaggacggg cgttgcggcc ttttttgaca ccaatttcaa
      661 ccagttggcc aaccccttcc tggtcaagca cctggaacgg atcgtctttg agaatcttgt
      721 tttcgacata gaacggcaga aacgttccgg aatcgtcgcg cgacaaccca aacgtcgtct
      781 gcgtcaggtc gttggtccca aagctaaaga attcagcagc cgtggcaatc ctgtcagctg
      841 tgagcgccgc ccgtggcagt tcgatcatgg tgccgaccag gtagtcaaat ttttcaccag
      901 tcgcatccat cacttcgttc gccactcgac ggacaatgtg ttcctggcgg acaaattcct
      961 ggacatcgcc aaccagtgga atcataactt ccggcaagac tttgacatcg tgttgttgca
     1021 cgtgtgcggc ggcttcaaaa atggcccggg cctgcatttc cgtgatttcc ggatagacaa
     1081 gtccgagtcg gcagccacgg tgaccaagca tcgggttggc ttcgcggagc gcttcgattt
     1141 tggcttcgac cactttcggc gtcaggccga cttttttggc cagcagtttc atatcttcca
     1201 ccgaatgcgg caggaattca tgcaacggcg gatccagcaa gcggattgtg accggtaacc
     1261 cattcatcgc agtgaagata ccaatgaaat cttcccgttg catcggcagc agcgccgcca
     1321 gtgcccccag gaaaagctgt ttgggtttga cgattttttt gtcgagtttg gcttgttgtt
     1381 tcttgagttc agccagtttc tcttttccgc caccggcttt ggccgtttcc ttggcaattt
     1441 cctgatggat cagcgaccgt tcagattcaa gccgcttgta ctctgaagcc accataatca
     1501 tttgccggac catatcaatg cggttgcctt caaagaacat atgttcggtg cggcacaacc
     1561 cgattccttc agccccgaat ccacgtgcca cgtgtgaatc gtgcggtgtg tcggcattgg
     1621 ttcggacatt gagtgttcgg aattcatcgg cccagctcat aaactcgcgg aaatcgccgg
     1681 tgatttcagg ttcaatcaac gggacctggc cttcgatcac gcgcccggtt gaaccatcga
     1741 gcgtgatgta ctccccttct ttgatcacac ggtcaccaac tttaaactgc cgggcgtcat
     1801 aatcaatttc gaggcttccg caaccggcaa cacaacattt gcccatccca cgggcaacg
//
LOCUS       DAOQDO010001417         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64439_length_1859_cov_2.310976, whole genome shotgun sequence.
ACCESSION   DAOQDO010001417 DAOQDO010000000
VERSION     DAOQDO010001417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64439_length_1859_cov_2.310976"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="PKZ53_19375"
     CDS             <1..1782
                     /locus_tag="PKZ53_19375"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNJ42657.1"
                     /translation="NTLRLWSAKASRDFDFTYFNEGDYIGAVEAKNDSETISKVLYPN
                     DNRVSGKELRLKQEYFFVSATLQDIMRRYKKAHTTFDQVPEKIAIQLNDTHPAIAIAE
                     FMRILLDQEHLEWDKAWKMTTQVFGYTNHTVMPEALEKWPVSLLGKVLPRHLQIIYEV
                     NHQFLQQVRALHPNDDNLCARMSLIEEPSQPGGEKHVRMAYLAIVGSHSTNGVAALHT
                     EILKNDLFKDFYQMFPEKFNNKTNGITQRRWLKMCNMKLSALINDAIGDEWTTNLDHL
                     KKLIPFAEDAEFRKQWQAVKRHNKKRLAGLIKGDTSLDSIFDTQVKRLHEYKRQLLNV
                     LHVITAYNRIRENRNVDYVPRTVIFGGKAAPGYVMAKLIIKLIHAVGDVVNQDPAVRG
                     LLKVVFLPNYRVSLAERIIPGTDLSEQISTAGTEASGTGNMKFALNGALTIGTLDGAN
                     IEMLEEIGEDNMFIFGLKADEVRELRKTYNPKDYYNQNTELKQVLDMIQNGYFSPLHR
                     DLFDPIIHSLLYSGDQYMLLADYAAYIECQDRVSEVYRDQEEWTRRSILNVANVGKFS
                     SDRTIRQYAEEIWNASPVTVDMSTCLL"
BASE COUNT          510 a          486 c          430 g          433 t
ORIGIN      
        1 aacacgttgc gactctggtc agccaaggca tcgcgtgact ttgatttcac ctactttaac
       61 gaaggtgatt acatcggtgc agttgaagcc aaaaatgatt ctgaaaccat ctccaaagtg
      121 ctctatccaa atgacaaccg ggtttctgga aaggaattgc ggctcaaaca ggagtacttc
      181 ttcgtttcgg ccacgctgca agacatcatg cggcggtata aaaaagccca taccacgttt
      241 gaccaggtac ctgaaaaaat tgcgattcag ctcaatgaca cccatccggc aattgcaatt
      301 gccgaattta tgcgaatttt gctggatcag gaacatctgg aatgggacaa agcctggaaa
      361 atgaccacac aggtgtttgg ctacactaac cacacggtaa tgcccgaagc attggaaaaa
      421 tggccggtaa gcttgcttgg aaaagtcctg ccccgccacc tgcaaatcat ttatgaagtc
      481 aaccatcaat ttttgcagca agttcgggca ctccatccca atgacgataa cctgtgcgcc
      541 cgcatgtcac ttatcgaaga accatctcaa cctggcggtg aaaaacacgt tcggatggcc
      601 tatctggcaa ttgtcggcag ccacagcacc aacggcgtcg ccgccctgca cactgaaatc
      661 ctcaaaaatg acctctttaa ggatttctac cagatgtttc ccgagaaatt taacaacaaa
      721 accaatggca ttacccagcg tcgctggctc aagatgtgca atatgaagct gtcagccctc
      781 atcaacgacg ccattggcga cgaatggacc accaacctcg atcatttgaa aaaactgatc
      841 ccgtttgctg aagatgctga atttcgcaag caatggcagg ccgtcaaacg ccacaacaaa
      901 aaacgactgg ccgggctcat caagggcgat accagcctgg actcgatttt tgacacccag
      961 gtcaagcggc tccacgaata caaacgccag ttactcaatg tgttacacgt cattacggcg
     1021 tacaatcgga ttcgtgaaaa ccgcaatgtg gattatgtgc ctcgaacggt gatcttcggt
     1081 ggcaaagcgg caccagggta tgtcatggcc aagctgatta tcaaactgat ccatgcggtc
     1141 ggtgacgtcg tcaatcagga tccagccgtt cgtggattgc tcaaagtggt ctttttgcca
     1201 aattaccgcg tctcgctggc agaacgaatc attcccggaa ccgatctttc cgaacaaatt
     1261 tcaactgccg gaaccgaagc ttcaggtacc ggcaacatga aatttgccct caatggcgcc
     1321 ctcaccatcg gtacactcga tggtgccaac atcgaaatgc tcgaagaaat cggcgaagac
     1381 aacatgttca tctttgggct caaggcagat gaagtccggg aactccgaaa aacctataac
     1441 ccaaaggact actacaatca gaatactgaa ctcaaacagg tactggatat gatccagaac
     1501 gggtacttca gcccgctcca ccgtgatctg tttgacccaa tcatccattc cctgctgtat
     1561 tcgggcgacc aatatatgct gctggccgat tatgcggcct atattgaatg ccaggaccgg
     1621 gtgtctgaag tctaccgcga tcaggaagaa tggacccggc gttcgatttt gaacgtggcc
     1681 aatgtcggca aattctccag tgaccgcacc atccggcagt atgctgaaga aatctggaat
     1741 gcttcgccgg tgacggttga tatgtcaacc tgcctgctct aaagccatga atgaagaaag
     1801 tggctagtgc ctggttcttg gttcttggtt cttggttctt ggttcttggt tcttggttc
//
LOCUS       DAOQDO010001418         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64441_length_1859_cov_2.257761, whole genome shotgun sequence.
ACCESSION   DAOQDO010001418 DAOQDO010000000
VERSION     DAOQDO010001418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64441_length_1859_cov_2.257761"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1859
                     /locus_tag="PKZ53_19380"
     CDS             <1..>1859
                     /locus_tag="PKZ53_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ42658.1"
                     /translation="PHLVEPLRVLSQSEGCTMFMTLLAAFAVQLSRYTGQQDLLIGTP
                     IANRTRSEIENLIGFFVNTLVIRVNLSGKPGFRDVLRQIRETALGAFAHQDVPFEKLV
                     ELLHPDRDLNRTPLFQVMFAFGQEGRASQKSAPPNRTQLIDTLEVENRTSKFDLTLSM
                     AETEAGIIAGFEYKTELFESATIRRFLTHLETLLHGICTNPEAPISSLPLMSEAERDQ
                     VLVQPNQTERQFPTDKLLHHLIEIQVRQTPDAIAVVSGDQNVTYHRLNSQADVLAQYL
                     QHLGVGPGALVGVLVERSIEMVTALLGVLKAGGAYVPLDPGYPADRLAFMISDAQASL
                     ILTQSHLVERLPHTSAQTICLDQPFSLPARPSNTLPAAQPVPEDLAYVLYTSGSTGQP
                     KGVMIPHRAICNHMHWMHQTFPLSADDRVLQKTPFSFDASVWEFWAPLMAGAHLVMAE
                     PGIHQDAESLSQVIADQQITILQVVPTLLSLLLEEPLFSQCPQLRRVFAGGEALSGNL
                     RDRFFEKLPDVELVNLYGPTECTIDATFFVCQPDNSSRTVPIGKPVSNMTALVLDAEG
                     HPVPIGIPGELYLGGVGVGCGYLRRPELTQERFALRAGGWGLGAEGFQFKAIE"
BASE COUNT          413 a          527 c          482 g          437 t
ORIGIN      
        1 ccacatctgg tcgagccatt gcgcgtgctt tcacaaagcg aaggctgcac catgtttatg
       61 accctgctgg cggcctttgc ggttcaactc agccggtaca ccgggcagca agaccttctc
      121 attggcaccc caattgccaa tcggacccga tccgaaattg agaatttgat tggttttttc
      181 gtcaatacac tggttatccg ggtcaatctc tccggtaaac ctggatttcg ggacgtgctc
      241 cggcaaattc gggaaacggc gctgggtgct tttgcccatc aagacgtgcc atttgaaaaa
      301 ctggttgaat tgctccaccc ggatcgggac ctgaaccgca cgccgctctt tcaggtgatg
      361 tttgcctttg gccaggaagg ccgcgccagt caaaaatccg caccacccaa tcgaacccag
      421 cttattgaca cgcttgaagt tgaaaaccgt acctccaagt ttgacctgac tctgtcaatg
      481 gcagagaccg aagccgggat tatcgccggg tttgaataca aaacagaact ctttgagtca
      541 gcgaccattc gccggtttct aacccacctg gaaacgctct tgcacgggat ttgtaccaat
      601 ccggaagcac cgatttccag cctgccgttg atgtcagaag ctgagcgcga tcaggttctg
      661 gtacaaccaa accagaccga gcgacaattt ccaactgaca aactgctcca tcatctaatt
      721 gaaatccagg tgcggcaaac gcctgatgcc attgcggttg tttctggaga tcagaatgtc
      781 acataccacc ggctcaattc acaggctgat gtactggccc aatatcttca gcatctgggc
      841 gttggacctg gcgcactggt gggagtcttg gttgaacgct caattgagat ggtgaccgca
      901 ttactgggtg tgctcaaagc tgggggggcc tatgttccac ttgacccagg atatccggct
      961 gatcggctgg catttatgat cagcgatgcc caggcatcac tgattctgac ccaatcgcac
     1021 ctggtcgaac ggctgcccca cacctcagcc cagacgattt gcctggatca gccattttcc
     1081 ctgccagcca ggcccagcaa cacccttcct gccgcgcagc cagttcctga agatctggct
     1141 tatgtgctgt acacgtccgg gtcaaccggt cagcccaaag gtgtaatgat tccgcatcgg
     1201 gccatctgca accacatgca ctggatgcac cagacctttc ccctctccgc cgatgaccgg
     1261 gtgttgcaaa aaacaccgtt tagttttgat gcttcggtgt gggaattctg ggcaccattg
     1321 atggctggtg cccacctggt gatggctgaa ccaggaattc atcaggatgc ggaaagcttg
     1381 tcgcaagtaa ttgcagatca acagattacc attctgcaag ttgtcccgac cctgctttcc
     1441 ctgctgcttg aagaacccct gttttcccag tgccctcaac tccgacgggt ttttgccggt
     1501 ggcgaagcgc tttccgggaa cctgcgggat cggttctttg aaaaacttcc agacgtggaa
     1561 ctcgtcaacc tgtacggacc aaccgaatgc acaattgacg ccaccttttt tgtctgccag
     1621 ccagacaatt cgagccgcac cgttcccatt ggaaagccag tttcaaatat gacggcgctg
     1681 gtgcttgacg ctgaagggca ccccgttccg attggcattc ccggcgaact ttacctgggc
     1741 ggcgtgggcg tcggctgtgg atatttgaga aggccagagc tgacacaaga gcgcttcgcg
     1801 ctcagggctg ggggctgggg gctgggggct gaaggttttc aattcaaagc gattgaaaa
//
LOCUS       DAOQDO010001419         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64495_length_1858_cov_3.590682, whole genome shotgun sequence.
ACCESSION   DAOQDO010001419 DAOQDO010000000
VERSION     DAOQDO010001419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64495_length_1858_cov_3.590682"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1858)
                     /locus_tag="PKZ53_19385"
     CDS             complement(<1..>1858)
                     /locus_tag="PKZ53_19385"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNJ42659.1"
                     /translation="WTVYDTTSGLPSNLVRNLMVSSQGTGSPTLWVGTEDGGLSRLES
                     PAWKPVLFPPNLIKNRLQCMLHTQSASGEPVLWVGTFGDGLLQYSNHSWKVFTESTGL
                     PSNKIRCLLETVDARGQSTIWVGTNNGLARYQNGTWTTFTTSNGLADNVVFCLLETTT
                     GDQTTLWVGTGGGLSRLSNNTWSTVLPGKQSTMGSVRSLLETQTKTGDRALWAGTING
                     LWCYLNGSWTKRDAQSGLKKNYIRSLIEIKLKTSARYLLIGTDGGGILGLDPGSGLSA
                     STPIKTLVSEQTSPSISGNVIYHLFQHGASDVYALTIRGVVRLKIPTLDPKDLWVDEF
                     SSVDGLPGNECNGTAGLVDKIGRIWAATTRGLAVLDPTRQWTDSSRKPLSIERLTVSG
                     DEFKLGQVQYSTSNPLILDYTQNQIKIEIALLCFVREAETRYQTQLVGVDPKPTAWST
                     DYDREYSNLGAGQYVFRASGKDYAGNVTGPVEIVFQITPAPWRTWWAYLIYVVVGSVL
                     VVGSVFLRLRTLHLRTLHLQHLVTERTTEVLQQKQQLEITNQSLEGANHELKLLNEMK
                     TEFLGIAAHDLKNPLTIIMTYAELLSIDPNSRPTTVNMAQAIFTSAKRMLGII"
BASE COUNT          460 a          462 c          485 g          451 t
ORIGIN      
        1 gataattccc agcatccgct tggctgacgt aaagatagcc tgggccatgt taactgtggt
       61 tggccgcgag ttggggtcaa tcgaaagcag ctcagcataa gtcatgatga tggtgagcgg
      121 gtttttgagg tcatgggcgg caatacccaa aaactcagtt ttcatttcat tgaggagttt
      181 gagttcatgg ttggcacctt caagtgattg attggtaatt tcaagttgtt gtttttgttg
      241 gaggacttcg gtggttcgtt cagtcaccag gtgttgcagg tgaagggtgc gcaggtgaag
      301 ggtgcgcagg cgaagaaaca ccgagccaac taccaaaacc gagccaacca caacgtagat
      361 caaataagcc caccaggtac gccagggagc tggcgtgatt tgaaacacaa tttcaaccgg
      421 tccggtgaca ttgccagcat aatctttacc cgaggctcga aagacatatt gaccggcgcc
      481 cagattgctg tattcccggt cataatccgt agaccaggcg gtgggtttgg gatcaacacc
      541 aaccagttgg gtctggtatc ttgtttcagc ttcgcgcaca aaacacagca gggcaatttc
      601 aattttgatc tgattttggg tgtaatcgag aatcagggga ttactggttg agtattgtac
      661 ctgcccaagt ttgaattcat caccagaaac cgtgagccgc tcaatagata atggctttcg
      721 tgaactatca gtccactgcc gggttgggtc caaaacagcc agtccacgcg tggttgccgc
      781 ccagattcgt ccgatcttat ccaccagacc tgccgtccca ttacattcat tgccgggcaa
      841 cccatcaacc gaggaaaatt catcaaccca taaatctttt ggatccagtg tgggaatttt
      901 cagccgaacg acaccgcgaa tggtcagggc atatacatca cttgccccat gttgaaacag
      961 gtggtagatc acgttgcctg aaatggacgg tgaggtctgc tcagaaacca gggttttgat
     1021 cggcgtggaa gctgagagcc cggaaccagg atcgagcccc aaaattcctc cgccatcagt
     1081 accaatcaac aaatagcggg cactggtttt gagtttgatt tcgatcagac tgcggatgta
     1141 gttcttcttc agaccagact gggcatcgcg tttggtccag gatccattga ggtaacacca
     1201 gagcccattg attgtgccag cccaaagcgc ccggtcaccg gttttggttt gggtttccaa
     1261 aagtgaccga acggaaccca tcgtggattg tttacctggc aagaccgttg accaggtgtt
     1321 attggaaagt cgggataacc cacctcccgt accgacccac aatgttgtct gatcgccggt
     1381 tgtggtttcc agcaggcaaa aaaccacatt gtcggccaat ccattcgagg tggtgaatgt
     1441 ggtccaggtg ccgttttgat agcgagccag cccattatta gttccaaccc aaatcgttga
     1501 ctgacctctg gcatccactg tttcaagcaa acaccgaatt ttattgctcg gcaacccggt
     1561 gctttctgta aaaaccttcc acgaatgatt ggagtattgg agcagtccat caccaaatgt
     1621 tccaacccag agcactggct cgcccgaagc ggattgggtg tgcaacatgc attgcagccg
     1681 atttttaatc aggtttggag gaaataaaac tggcttccag gccggggatt ccaggcgaga
     1741 cagcccgcca tcttcggtgc caacccagag cgttgggctc ccggttccct gggaagaaac
     1801 catcaggttt cggaccagat tgctgggcag gccagaggtc gtatcataaa ccgtccag
//
LOCUS       DAOQDO010001420         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64508_length_1858_cov_3.105935, whole genome shotgun sequence.
ACCESSION   DAOQDO010001420 DAOQDO010000000
VERSION     DAOQDO010001420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64508_length_1858_cov_3.105935"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="rimP"
                     /locus_tag="PKZ53_19390"
     CDS             complement(<1..485)
                     /gene="rimP"
                     /locus_tag="PKZ53_19390"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNJ42660.1"
                     /translation="MRSQPQPSDSKNHDCDPKLPSSRVDEQVKELIEQVITSAGFEFV
                     HCEVTDESKQRVLRVLVDHPEGITLNHCSYLSHQIGLQLDQADLIPQTYLLEVSSPGV
                     ERGLYRLKDYQRFAGHQIKLRTSHPIDGSRNFRCTLLGVREPEVSPEPSSGPAEPMVM
                     IS"
     gene            709..1587
                     /locus_tag="PKZ53_19395"
     CDS             709..1587
                     /locus_tag="PKZ53_19395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ42661.1"
                     /translation="MRKRRPRAVSQHKLARRDELLVLTAGFLKRVQVDFKETYAFEVT
                     HQPIYLPHLPSEFEGFRIVQLSDIHHSPYLTLEHLSEAVAIANNLEPDLIVLTGDYIT
                     HTAVYIEPCAERLGDLKAKHGVYAVLGNHDVWVNARAVTKAFERHHIHVLTNVNTEIS
                     IRGSSIWLSGIGDLMMHFHDLPAALKGTTSKFPRILLSHNPEIIEEAAYSNVDLVLAG
                     HTHGGQFQLPLIGSPMALTRYGRRYARGLAAMQDTQIYVNRGLGTVFVPVRYQCPPEI
                     SLLELTADPLKPLMSE"
BASE COUNT          418 a          472 c          480 g          488 t
ORIGIN      
        1 gagatcatga ccatcggctc ggcgggaccg gatgaaggct ctggagaaac ctccggttcg
       61 cgcacaccaa ggagtgtgca ccggaagttg cggctgccat caattgggtg ggaggtgcgg
      121 agcttgattt ggtggccggc aaaacgctga taatccttga ggcgatacag gccgcgttcg
      181 acgcctggtg aggagacttc caacaaataa gtctgaggga tgagatcagc ctgatccagt
      241 tgaagtccaa tttggtggct tagatacgag caatggttga gtgtaattcc ctctgggtgg
      301 tcaaccaaga cccgtagcac gcgttgcttt gattcatcgg tcacttcaca gtgaacaaat
      361 tcaaagcccg ctgaagttat aacctgctcg atcaattctt ttacttgctc gtccacgcgt
      421 gatgaaggga gtttggggtc acagtcgtga tttttcgaat ctgaaggttg cggctgtgaa
      481 cgcaaacctg agatctgctg tatgtaaatc aaggtgggcg ttgctgccca cctttgcgta
      541 agcctttaag tatatatgcg gtgtgtgaag agggcaagta gattgtgcac aacctcgaat
      601 ggtgtcacac ctggcctgta agatatggta ctatacagcc tgctccccct ggtctttcct
      661 gctggttagg atgagttcta ctctggctcc gtacaaggga taaccactat gcgcaaacgc
      721 cgaccccgtg ccgtttctca acacaaattg gcgcgccgag acgaattgct ggttttgacg
      781 gctggctttt tgaagcgtgt ccaggttgat tttaaagaaa cttacgcctt tgaagtcacc
      841 catcagccga tttatcttcc gcacctgccc tctgaattcg aagggtttcg cattgttcag
      901 ctttcagata ttcatcatag cccatacctg acactcgaac atctgtctga agccgtagcg
      961 attgccaaca atctggaacc ggacctgatt gtgttgaccg gggattacat tacgcacact
     1021 gcggtctata tcgagccatg tgctgaacgg ttgggggatt tgaaagccaa acatggcgtg
     1081 tatgcggttt tggggaacca cgatgtctgg gtcaatgccc gtgccgtcac caaagccttt
     1141 gaacgccatc acatccatgt actgaccaat gtcaacaccg aaatttcgat tcgcgggtca
     1201 tccatctggt taagtggcat tggtgatttg atgatgcact ttcacgattt accagcggcc
     1261 ctcaaaggaa ccacgtcaaa gtttccccgc attctgcttt cacataaccc ggaaattatt
     1321 gaagaagcag cctattcaaa tgttgatctg gtgctggccg gccataccca tggcggacaa
     1381 tttcagcttc cgttgatcgg gtcgcccatg gcgctcaccc gatatggacg acgctatgcc
     1441 cgtgggttgg cggcgatgca ggatacacaa atctatgtca atcgcgggct tggcactgtg
     1501 tttgtcccgg tgcgctatca atgcccaccc gagatttcgt tgctcgaact cacggctgac
     1561 ccgctcaaac ctttgatgtc tgagtgactg gcctgtctgg ctccagccga aagttaccac
     1621 ctctcagcaa aagagttctt tgagcgagtg cgtcaagccg ccgaattcca gaatattttt
     1681 cctgattgcc cctgctgtga taacgcgata tgtctgagag tttccgccag ccaacatcat
     1741 ttgagatttc acgacaggtt ctcacctggc ccaatctgat taccacgttc cggattatgt
     1801 tgattccagt ctttctgcgc tgcattttga ttgggcgcta tgactgggcg ctcacggt
//
LOCUS       DAOQDO010001421         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64517_length_1858_cov_2.738214, whole genome shotgun sequence.
ACCESSION   DAOQDO010001421 DAOQDO010000000
VERSION     DAOQDO010001421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64517_length_1858_cov_2.738214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..943
                     /locus_tag="PKZ53_19400"
     CDS             326..943
                     /locus_tag="PKZ53_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42662.1"
                     /translation="MENINQLYLLYSTWALYVGTLSLLGGLWLVSALFSLRLLFVLVS
                     WEKFIARHSLTFSRQRLVIAFISLLGITGIWTYLIIKPDQKFYSYRGEKAILQGNYSQ
                     AAYYYRKLVDWGDTSLVSLNNLAICSYQLGDFPEAIRLFSLVHQQSPLISPQSYILCA
                     QAYLKIGQPQQAQGLLQKAMTLSWPPPQKEKLRSYLDKLQKEPHN"
     gene            945..>1858
                     /locus_tag="PKZ53_19405"
     CDS             945..>1858
                     /locus_tag="PKZ53_19405"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ42663.1"
                     /translation="MSFNKIWRWLRPGEYLVLVWIAIVSVIYSVYGQWGKFWNETIDF
                     GPFIIPFLLTFSFYCVPLIGTLLKKLYASVVFILNPTREELVQDLIRFTEEKKYLTGT
                     VTFLRDCLPVYLFIIFYPSTNLLLETLQGPRTLDAELVYLDHLIFRVHLSIWMESFIS
                     RGLTEVMAFFYSLHMILVAFVLCYLYFFAKRELFLEATQGFMLICMIGLTGYVLVPAV
                     GPTFSLAHLYTRDLSMGFLAQTNYVITESLRVPRDCFPSLHVGYSALMLVYAWRANKW
                     FGYFLLPLDVGNWISTIYLRYHYAIDVVA"
BASE COUNT          465 a          403 c          380 g          610 t
ORIGIN      
        1 accggtcacc catcaggatg taatccagaa tcaccagcaa cccgagatcc ttttgtgact
       61 gccggttcaa aaaagacggc aaccacacca gcgtcaggct tccctctggt ccggcttttg
      121 agaatttcgc caatagtcag ttcttcggta gtgttccaaa actgttgaag ctcaaaaaac
      181 tggatttgga tgacttatcg gaacaacaac caaatcaacg gcttggaatc aagatcggca
      241 actccaaaaa gatgttttca atagaatcag aacactgccg aatttctttg attcgaacgt
      301 ttttgactca atatctgtca cggctatgga gaacataaat caactttatc tgctttattc
      361 tacctgggca ttatacgttg ggactttgtc actgttgggc gggttatggc tggtttcagc
      421 tcttttctca ctccgattgc tttttgtgct ggtttcgtgg gaaaaattca ttgcccgaca
      481 ctctttgacc ttttcacgcc aacgcctggt tattgccttt atttctctgc ttggaataac
      541 agggatatgg acatatttga tcataaagcc agatcagaag ttttattctt atcgtggtga
      601 gaaagcgatt ttgcagggga attactcaca agcggcttac tactatcgga aactggtgga
      661 ttggggagat acctcgcttg tgtctctgaa caacctggca atatgcagtt atcaattggg
      721 ggattttcct gaagcgatca ggctattttc tctggttcat caacaatctc cactaatttc
      781 tccccaaagt tatattttat gcgcccaggc ttacctcaaa attggtcagc cccaacaggc
      841 acaaggtctc ttgcaaaaag cgatgactct ttcctggccc cctccacaaa aagaaaagct
      901 tcgttcctac cttgataaat tgcaaaagga gccacacaac tgaaatgagt tttaataaaa
      961 tttggagatg gcttcgtcca ggtgaatacc tcgttttggt atggattgcc attgtctcag
     1021 tcatttactc agtttatggc cagtggggaa aattttggaa tgaaacaatc gattttgggc
     1081 catttatcat tcctttcttg cttacattta gtttttattg tgtgccgttg atcggaactt
     1141 tacttaaaaa actatatgct tcggtagttt ttattctcaa tccgactcgt gaagaacttg
     1201 ttcaagattt gatcaggttc actgaggaga agaaatatct gacgggaacg gtcacctttc
     1261 ttcgagattg cctcccagtt tacctgttta ttatttttta ccccagtacc aacttgctgc
     1321 tagagactct acaaggccct cgtacactgg atgcggaatt agtctacctg gatcatttaa
     1381 tatttcgggt tcatttatca atctggatgg aatcctttat ttctcgaggg ttaactgagg
     1441 tcatggcttt tttttattca ctgcacatga ttttagttgc ctttgtgctc tgttatctgt
     1501 atttctttgc gaaacgggaa ctgtttctcg aagcaacaca gggctttatg ttaatttgta
     1561 tgattggact tactggatat gttcttgtcc cagcagttgg gccaaccttc tccctggctc
     1621 atttatacac ccgagattta tccatgggat ttctggcgca aacaaattat gtcataacag
     1681 aatcactccg ggttccacga gattgttttc caagtcttca cgttggatat tcagccttga
     1741 tgctggttta tgcgtggcgc gccaataaat ggtttggcta ttttcttctt cctttagatg
     1801 ttggaaattg gatttccacg atttacctcc gatatcacta cgcgattgat gtcgtagc
//
LOCUS       DAOQDO010001422         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64621_length_1856_cov_3.732371, whole genome shotgun sequence.
ACCESSION   DAOQDO010001422 DAOQDO010000000
VERSION     DAOQDO010001422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64621_length_1856_cov_3.732371"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /gene="coxB"
                     /locus_tag="PKZ53_19410"
     CDS             complement(<1..1063)
                     /gene="coxB"
                     /locus_tag="PKZ53_19410"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNJ42664.1"
                     /translation="MAFLLPPFFPEQASTAAAEIDYFYFFMLAVCGGVGLTIMICITV
                     FAIKYRRRYEGQLAIETHESPWAEWGWTIVPFFVFMVMFYWSAIIFFKHTTIPKNTLD
                     VYVTGKQWMWKFQHLDGQREINQLHVPIGRPIRLIMSSEDVIHSFFVPSFRIHMDVLP
                     KKTSYTWFEATKTGTFHLFCSQYCGTEHAGMIGSVIVMEPQDYEKWLITNSQGSMAIR
                     GQELFNKLACNACHTGNAQARAPYLNNLYGKPIILETGDQVKVDENYIRESIYKPNAK
                     IAAGYANIMPSYEGQLTEEEIQLLVAYIRAMGNNLDQMPPVSNPTGVQPYPAGGNPDG
                     SLPYKAGQANQTPATGVPAP"
     gene            complement(1068..>1856)
                     /locus_tag="PKZ53_19415"
     CDS             complement(1068..>1856)
                     /locus_tag="PKZ53_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNJ42665.1"
                     /translation="HASQASTGSPPTSNSSVELLKQIGIEQHNGEQLPLDLEFFDENG
                     MPVKLGQYFTTQPVIIVPVLLKCQFLSLNVLNGLIRSLTDVKPGVGQDFTVLVVTIDP
                     RETTATAFGQRRGFIKRYGRPGSDPGWHFLTGRQPAIESLMKALGFQYIWNEEKQGFA
                     HSGGIIIATPQGKISRYFFGMEYPAETVTQALSTAQAGRVGTLVPASSPYCFHYDPGT
                     GKYTPVVLNLLKISAVVTVVALGFFLGMMLRSERLGHNTSEQRG"
BASE COUNT          441 a          468 c          493 g          454 t
ORIGIN      
        1 tcggggcagg aacacccgtc gcgggtgttt gatttgcctg accagcttta tatggcaatg
       61 aaccatctgg gttgcccccc gccggatagg gttgaacccc ggttgggttc gacactggcg
      121 gcatctggtc cagattattg cccatcgccc ggatgtaggc aaccaggagc tggatttcct
      181 cttccgtgag ttgcccttca tagctcggca tgatgttggc atacccggcg gcaatcttgg
      241 cgtttggttt gtagattgac tcacgaatgt agttttcgtc taccttcacc tgatcgccgg
      301 tttccaggat aattggcttt ccatagagat tgttcaggta cggcgcccgt gcctgagcat
      361 tgcctgtgtg acaggcattg caggcgagct tgttgaacaa ttcctggccg cgaatcgcca
      421 ttgatccctg agagttggtg atcaaccact tttcatagtc ctgtggttcc atcacgatca
      481 cgctgccaat catcccggcg tgctctgtac cgcaatattg ggaacagaac aaatggaagg
      541 tgccggtctt ggtcgcttca aaccaggtgt aactcgtctt tttcggcaac acgtccatgt
      601 gaatgcggaa cgacgggaca aagaagctgt gaatcacatc ttctgatgac ataatcaacc
      661 ggattggtcg cccgattgga acgtgcaact ggttgatttc gcgctggcca tccaggtgct
      721 gaaacttcca catccattgt ttcccggtga catacacatc cagtgtgttt ttggggatgg
      781 ttgtgtgttt aaagaaaatg atggctgacc agtaaaacat caccatgaag acaaagaacg
      841 gcacaatggt ccatccccac tcagcccagg gcgattcgtg ggtttcaatg gccagttgcc
      901 cttcataccg acggcgatat ttgatggcaa acaccgttat gcaaatcatg atggtcagac
      961 ccacaccacc gcacaccgcc agcatgaaaa agtaaaagta gtcaatttcg gcggcagccg
     1021 ttgaggcctg ttctgggaaa aatggaggaa gtaagaaagc cactgactta acctcgctgt
     1081 tctgatgtat tgtgaccgag tcgctctgat cgaagcatca tcccaaggaa gaacccgagg
     1141 gcgacgacgg ttaccactgc actgattttc aagagattca gtacaaccgg ggtatatttt
     1201 ccagtgcccg ggtcatagtg aaagcagtag ggtgaggagg ctggcaccaa tgttccgact
     1261 cgacccgcct gggcagttga aagcgcttga gtgacagttt cggctgggta ttccatgccg
     1321 aagaaatagc gtgaaatttt cccctgaggg gtggcgatga tgatgccgcc ggaatgggca
     1381 aacccttgtt tttcttcatt ccaaatgtac tgaaatccga gggctttcat caaagattcg
     1441 attgccggtt gccgtccggt caagaaatgc cagccgggat cacttccagg acgcccatac
     1501 cgtttgataa atccccgacg ctgaccaaac gcagttgcgg ttgtttcgcg cgggtcaatg
     1561 gtgaccacca gaaccgtgaa atcctggcca acacccggtt tgacgtctgt caggctccga
     1621 atcaaaccat tgagcacgtt caggctcaaa aactgacatt tcaacaacac cggaacaatg
     1681 atgaccggct gagtggtgaa atactggccg agtttgaccg gcattccgtt ttcgtcaaag
     1741 aactcaaggt caagcggaag ttgctcgccg ttgtgctgtt caattccaat ttgcttgagg
     1801 agttcaaccg acgaatttga agtcggcggc gaaccggttg aggcttgact ggcatg
//
LOCUS       DAOQDO010001423         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64684_length_1855_cov_3.266667, whole genome shotgun sequence.
ACCESSION   DAOQDO010001423 DAOQDO010000000
VERSION     DAOQDO010001423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64684_length_1855_cov_3.266667"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="ruvB"
                     /locus_tag="PKZ53_19420"
     CDS             complement(<1..674)
                     /gene="ruvB"
                     /locus_tag="PKZ53_19420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNJ42666.1"
                     /translation="MAKTSKENQADHTDRLLTGESEGPGEQFTELSLRPKLLREYIGQ
                     SKSTDNLGIFMKAARNRKKALDHVLLHGPPGLGKTTLAMIVANEMGVEIKSTSGPVIE
                     KSGDLAALLTNLQENDVLFIDEIHRLSPAIEEILYPAMEDYQLDIMIGQGPSSRSIKL
                     DLPPFTLVGATTRAGLITAPLRGRFGITLHLDFYGEEHLTEIILRSAKILEVTIDQTG
                     ALEIARR"
     gene            complement(797..1195)
                     /locus_tag="PKZ53_19425"
     CDS             complement(797..1195)
                     /locus_tag="PKZ53_19425"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNJ42667.1"
                     /translation="MELPNQETPSKLFSLEEANRLVPTLRRLIKQVVKAQQGYTRVAD
                     DIERARDRATSGGGSHMGGYYIQCLAVFSKSVHDIEALGVLVKDYRVGLCDFPHLRDG
                     RVVYLCWKMDEDEVRWWHDIEAGFAGRQPL"
BASE COUNT          453 a          473 c          469 g          460 t
ORIGIN      
        1 cgacgggcaa tttcgagcgc accagtctgg tcaatggtga cttccaggat ttttgccgag
       61 cgcaagataa tttctgtcag gtgttcttcc ccataaaaat ccaggtgcag cgtaattcca
      121 aatcgtcctc tgagtggtgc cgtgatcaga ccagcccgag ttgttgcccc aaccagggta
      181 aagggcggca aatcaagctt gatagatcgg gaagacggtc cttgcccaat cataatatca
      241 agctgatagt cctccattgc tggatacaaa atttcctcaa tggctgggga cagccggtga
      301 atttcatcaa taaacaacac atcgttttcc tgcaaattgg tcagaagtgc tgctaaatca
      361 ccagatttct cgatcacagg tccagatgta gatttgattt caacgcccat ttcattggcc
      421 acaatcatag ccagtgtggt cttccccaat cctggcgggc cgtgaagcag aacgtgatcc
      481 agcgctttct tccgatttcg cgccgccttc ataaaaatcc ccaaattatc agtggatttt
      541 gattgcccga tatattcgcg caggagtttt gggcggagcg acaattcggt gaactgttcg
      601 cctgggcctt ccgactctcc tgtaagcagc cggtcagtat gatcagcctg gttttctttg
      661 gatgtctttg ccatacggtt gttagtgctt ggtgcttggt gtttggaagt acttggagcc
      721 acagagctaa gagccagtca ccaggcacca ggaatcacaa cgtgtctctt cattctcaat
      781 tcttcattct tcattcttaa aggggttgcc gcccggcaaa cccggcttcg atgtcgtgcc
      841 accagcggac ttcatcttca tccattttcc agcagagata caccacccgg ccatctcgca
      901 aatgcgggaa gtcgcacagg ccaactcgat aatctttgac caggacgccg agggcttcga
      961 tgtcgtggac acttttggag aagactgcca ggcattgaat gtagtatccg cccatgtggc
     1021 ttccgccgcc ggaagtggca cgatcacggg cccgctcgat atcatcggcc acacgtgtgt
     1081 acccttgctg ggctttgacg acctgcttga tcaatcggcg gagggttgga acgaggcggt
     1141 tggcttcttc aagggaaaag agttttgacg gggtctcttg gttgggaagt tccataacaa
     1201 aattcagggc tccaggcaaa atttccgggt aatagtacct gaagcaccgg gcacgagacc
     1261 agtggagatg gaaaatcctt ttgccgggct gaagattatc gggctgaaga caatgggctg
     1321 aagaagtcgg gctcagggta ttaaaacccg ttttcttctg ccctgagctt gcgagtcttc
     1381 agccccaagc cctgagccca gatttctcag tccgaaaaaa aagctccccg ccccctagcc
     1441 tggggtgagg agcttttaca tcaacaacgt ggctgcaacg cctgggggcg tagcaggtgc
     1501 atccaaacat cacgttgtga ggagaacctg agtgaagaat gaagaattga gaatgaagaa
     1561 tgaagaatca ggcatagggt tcagggttcg ggattttcga atggtgatcc ttatttacag
     1621 tttacaggcc gtaaacgacc tgccttaatt cagccaccgc tacacagcga aaaccaggag
     1681 acaatgaccg ggtgaaaagt gaggttttca tccctcagtt cttcgattgc ctcaaaccct
     1741 aaaatcagag ctgagggctg aagacaatgg gttgaagacc tggttttctt tcatccctca
     1801 tccctcatcc ctcatccctt ctgaaaaccc ggaaccctgt agttcaccgt tgaaa
//
LOCUS       DAOQDO010001424         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64689_length_1855_cov_3.022222, whole genome shotgun sequence.
ACCESSION   DAOQDO010001424 DAOQDO010000000
VERSION     DAOQDO010001424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64689_length_1855_cov_3.022222"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKZ53_19430"
     CDS             complement(<1..706)
                     /locus_tag="PKZ53_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42668.1"
                     /translation="MDQNTQKAEMKNLISRNISVGVAAKAFYMVTRVFLPPLILSHVS
                     LEEYGIWATCFILISYLGMSAFGVSNVYIRYVAEFRARQEYDKINRLLSTGMTFILVL
                     SLGLMAGLWFVLPMVISGFKISPALAPMAFQLIYITALTFVLDLSLGAFSYVLIGLQK
                     ITEQNLVWVATFCLEAVLIVVFLLTGFGIYSLLWAFVIRYVVAIVAYVVLCYRTLPTL
                     KLTLGQIDRESLRLFTG"
     gene            complement(759..>1855)
                     /locus_tag="PKZ53_19435"
     CDS             complement(759..>1855)
                     /locus_tag="PKZ53_19435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42669.1"
                     /translation="SSSTGEVVYIDTEWFAGPLYRLAKWLFPRSEHAVFLLSSLDFYV
                     YDRQATRILKKRMRAGESWDVVQSVTPVSPIAATTLHQLGLPIVIGPLNGGLGNPSNF
                     PELMAADSGWFYRVRDLGRLIDWWNGSSRNADIILTATQATRDYILPKWKSKCVPMLE
                     NGVELTRFCPTPWPDAPSAIHPLKILFVGRLIPVKGIPMLLRAVAGIRHEFPIEVTVV
                     GDGPMAQDWQHQAQALELSKIVTFTGNLSLDQVAVEMRKAHIFCLPSIRESGGAVLLE
                     AMASQRPIVAVAFGGPAEIVDDQVGAAIEPRGTEFVVSELQRIFRDVVANPAHWQRRG
                     EVGLERARERYGWDAKIQSALRLYHQLLAK"
BASE COUNT          515 a          492 c          449 g          399 t
ORIGIN      
        1 acccggtaaa gagccggagg gattcgcgat caatttgccc gagcgtcaat ttcaaagtgg
       61 gtaaggtgcg ataacacagc acgacgtagg ccacaattgc cactacatag cggatgacaa
      121 acgcccagag cagcgaataa atcccaaatc cagtcaacag aaaaaccaca atcagcacgg
      181 cttcaagaca gaacgtcgcc acccaaacca gattttgctc agtgattttc tgaagcccaa
      241 tcagcacata ggaaaaggcc ccaaggctta aatcaagcac aaacgtcaat gctgtgatgt
      301 agattaactg aaacgccatc ggtgccaggg ctggcgaaat cttgaatcca gaaatgacca
      361 tcggcagcac aaaccacaga ccagccatca accccaggct caggaccaga ataaaggtca
      421 ttccggtgga gagcagccgg ttgatcttgt catattcctg cctggctcga aattcagcca
      481 cgtaacgaat atagacattt gacacgccaa acgcgctcat cccaaggtaa ctgatgagga
      541 taaaacaggt tgcccagatg ccgtattctt ccaaactgac gtggctcaaa atcagtggcg
      601 gcagaaaaac ccgcgtcacc atataaaaag ccttcgccgc aactcccacc gagatgttgc
      661 gcgaaatcag atttttcatt tcagctttct gggtattttg atccataaaa tcaggccagt
      721 gcgtcagtag ttgttagatt gacgacttca ctagttcact atttcgctaa taattggtgg
      781 tagaggcgaa gtgcagattg gatttttgca tcccagccgt agcgttcccg agcccgttcc
      841 aatcccactt cacctcgcct ttgccaatgt gcagggttgg caaccacatc gcgaaaaatg
      901 cgttgcaatt ctgaaaccac aaattcagtc cccctgggct caatggcggc cccaacctgg
      961 tcatccacga tttcggctgg cccgccaaag gcaacggcca cgattgggcg ttgtgatgcc
     1021 atagcttcta gcaagaccgc gccgcctgat tcgcggatcg aaggtagaca aaaaatgtgg
     1081 gctttgcgca tttccacggc aacctgatca agggataaat tgcccgtaaa tgtcactatt
     1141 ttggatagtt ccagggcttg agcctggtgc tgccagtctt gagccattgg accatcacca
     1201 accacggtga cttcaattgg aaactcatgg cggattccag ccaccgcccg gagaagcatg
     1261 ggaatgcctt tgaccgggat caggcgacca acaaaaagaa tcttcagtgg atgaatagcc
     1321 gatggggcat ctggccaggg cgtcgggcaa aatcgggtca actcgacccc attttcaagc
     1381 attggaacgc atttagattt ccattttggc aaaatgtagt cacgcgtggc ctgagtggct
     1441 gtcaggatta tgtcagcgtt ccggcttgat ccattccacc agtcaatgag tcgtcctaga
     1501 tcacgaaccc ggtaaaacca gcctgaatca gccgccatca attcgggaaa gttggacgga
     1561 tttcccaatc cgccgttaag tggaccaatg acgattggca acccgagctg atgcaacgtg
     1621 gttgccgcaa tcggtgacac gggcgttacc gactggacaa cgtcccagga ttcaccagct
     1681 cgcattcgtt tcttcaagat tctggttgcc tgtcggtcat acacataaaa atcgagtgaa
     1741 gaaagcaaaa aaacggcgtg ttcactgcgt ggaaagagcc atttggccag tcggtagaga
     1801 ggtccagcaa accactcagt gtcaatgtac accacttcgc cggttgaact ggaaa
//
LOCUS       DAOQDO010001425         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64690_length_1855_cov_2.969444, whole genome shotgun sequence.
ACCESSION   DAOQDO010001425 DAOQDO010000000
VERSION     DAOQDO010001425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64690_length_1855_cov_2.969444"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKZ53_19440"
     CDS             <1..182
                     /locus_tag="PKZ53_19440"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ42670.1"
                     /translation="ACIPLKFNDRVVGVIAIFQLFSQKETLGVTDEELFEVLSSHAAT
                     ALYCSRLHPSVSSNG"
     gene            404..>1855
                     /locus_tag="PKZ53_19445"
     CDS             404..>1855
                     /locus_tag="PKZ53_19445"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNJ42671.1"
                     /translation="MSKSTHTLLNTAGRHVHLVVPFATVVILLVLLVPLPTWMLDILI
                     SFNLMLSVIVLLVSIYVLQPVQFTSFPSLLLLTTLFRLALNVATSRLILLNGEAGENA
                     AGSVIKAFGEFVIGGNYGVGIVIFLILLVIQFIVVNHGAVRSSEVMARFTLDAMPGKQ
                     MAVDADLAAGIIDEKVAKHRRAEINQAAEFHGSMDGAIRFTQRDAVASILITGVNILA
                     GFTLGVLQHDMSVVQAGKTYTILTIGDGVAAAIPSLLISVAAAIITTRAAHESHLSGD
                     ISAQLLLNPRPLYITAGVLAMLGILPGMPHVAFLTLALTVSGIGFLSSEYQQKILEKE
                     ALQSAQLAQEAAAEPSEPIEALTKLDVLALEIGYGLIPLVSSNPSFLARIREIRRQIA
                     VELGIIVSPVHITDNLQLPPNEYTILLKGERIASGEVRPDCLMAIDPGIVREQLEGTQ
                     TIEPVFKMPALWVNTMDRERALSAGYTVVDLVAT"
BASE COUNT          408 a          476 c          497 g          474 t
ORIGIN      
        1 cagcctgtat tccgctcaaa ttcaatgatc gcgtggtggg tgtgattgcc atttttcaac
       61 tcttctccca aaaagaaacc ctcggggtca cggatgaaga attgtttgaa gtattgagtt
      121 cacacgcggc aaccgccttg tactgttcca gactgcaccc atcggtatca agtaacggat
      181 aatagcagtt tgggctgagg gtattgggct aaggaatgga cacaaaatac cctggtcaac
      241 tgaaacaaac tctatcggaa gtcaaaaact caccacagag ggcacagagg aacatagagg
      301 gttcggttga ttgattttcc tctgtgttcc tctgtgtcct ctgtggtgaa aaaatgatga
      361 agttacttaa ttctcattcc gaagagctga gggcagtttt tgtatgtcga aatctaccca
      421 cactttgctt aatacggcag ggcgccatgt ccatctggtg gtgccatttg cgacagtggt
      481 gatcctgctg gtgctgctgg tgcccctgcc cacctggatg ctcgatatcc tcatcagctt
      541 taacctgatg ctgtcagtga ttgtgctgct ggtttcgatt tatgtgctcc aaccggtcca
      601 gtttacttcg tttccaagcc tgttattgct gacgacgctg tttcggctgg cattaaatgt
      661 tgccacttcc cgattgattt tgctcaatgg cgaagctggg gaaaatgcag ccggctcggt
      721 gattaaagcc ttcggagaat ttgtcattgg cgggaactat ggggttggca tcgttatttt
      781 tctgatcctg cttgtgattc aatttattgt ggtcaatcac ggcgcggtgc gctcatccga
      841 ggtcatggcc cggtttaccc tcgatgctat gccgggaaaa caaatggctg tggacgctga
      901 cctggccgct ggaatcattg acgagaaagt agccaaacac cgccgggccg aaatcaatca
      961 ggcggcggaa tttcatggct caatggatgg ggccatccgg tttacacagc gcgacgcggt
     1021 ggcctcgatc ctgatcacag gggtaaatat tctggccgga ttcaccctcg gtgtgttgca
     1081 acacgatatg tcagtggttc aggccggaaa gacctatacc attctgacga ttggtgatgg
     1141 ggtggcggcg gcaattccgt cgctgctgat ttccgtggcc gcagccatta tcacgacccg
     1201 cgccgctcac gaatcacatt tgagcggtga tatttccgcg caactgttgc tcaatccgcg
     1261 accgctctac attaccgctg gcgtgctggc gatgctgggt atcctccccg gcatgcccca
     1321 tgtcgcgttt ctgacactgg ccctgacggt gtctggaatt gggtttctct cttcggaata
     1381 ccagcaaaaa atactggaaa aagaggcgtt gcaatcagcg caactggctc aggaagcggc
     1441 ggctgagcca tctgagccaa ttgaagcctt gaccaaactc gatgttctgg cacttgaaat
     1501 tggctatggc ctgattccgc tggtgagttc aaatccgtca tttctggctc ggatccgcga
     1561 aatccggcgc caaattgcgg ttgaactggg catcatcgtt tcgccggttc acattactga
     1621 caacctgcaa ctccccccaa acgaatacac gattttatta aaaggcgaac gcattgcctc
     1681 gggcgaggtc cgcccggatt gcctgatggc gattgatcca ggcattgtcc gcgaacaact
     1741 cgaaggcacc caaaccattg agccggtgtt taaaatgccg gcgctctggg tcaacaccat
     1801 ggatcgcgaa cgggcgctct ctgccgggta caccgtggtg gacctggtgg cgacc
//
LOCUS       DAOQDO010001426         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64694_length_1855_cov_2.851111, whole genome shotgun sequence.
ACCESSION   DAOQDO010001426 DAOQDO010000000
VERSION     DAOQDO010001426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64694_length_1855_cov_2.851111"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..1817
                     /gene="hemN"
                     /locus_tag="PKZ53_19450"
     CDS             405..1817
                     /gene="hemN"
                     /locus_tag="PKZ53_19450"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNJ42672.1"
                     /translation="MSTAQLAQLTFGGATMNVPFHLIQKYNRPGPRYTSYPTIPEWST
                     DFKVEDFEQSIARTNQREQPRPVSLYFHLPFCESLCLYCACTTVITKNHAVVEPYLAQ
                     IKREVGWVSEHLDAGRQVEQIHFGGGTPTFLTPDQLADLYGAISSQYRISPTAEISIE
                     IDPRVTSPEHCRTLRELGFNRVSLGVQDFSPEVQKTVHRIQSYAMVKQLNDTCRDLGF
                     DSINIDLIYGLPHQTLESFLATVDQVIEIQPDRIALFSYAHVPWMKRQQKILEKHLPP
                     GEVKFEIFTQAIAKLTGAGYRYVGMDHFALEKDELCRAQDDRTLHRNFQGYTTKANCD
                     LFGFGMSSISGLEDTYAQHWHTLPNYYETAHKEAWPLMRGCQLTADDKLRRAVISRIL
                     CHTLLVKSEIEREFGICFDRYFAPELETLTELEQDQLVTVSSDRIEATPLGRILIRNI
                     AMIFDAYLKKPVEQRFSKTL"
BASE COUNT          477 a          420 c          475 g          483 t
ORIGIN      
        1 ttaaaacatt catagcagaa gggttcaggg ttcagggttt ctgaaaggat gagggatgag
       61 ggatgaggga tgaaagaaat caccttgtca ccttgtcagg atttggggtt cagggtttga
      121 gtagaatata ttttttgtaa tcattcaacc tgtggcgaac gcaaatcagg attttcagga
      181 agtactgatc ttcaagatgg gtgagatcat ttttccctca atactcatgc cagtttgttt
      241 tgtgctggta caggaattgc acaagccagc atgaaaacaa actcatgttg aatttgaaag
      301 ttgggttatg gggaagttat tttattgatt tttaatgatt tatcttgttc atagctcatc
      361 attcatcatt cataatttat tctcgcggtg aggaaaattt accaatgtct acggcacagc
      421 ttgcacaact gacgttcggt ggagcgacga tgaacgtacc gtttcacctg atccagaaat
      481 acaaccgacc ggggccacgt tacacgagtt acccgaccat tccggagtgg tccactgatt
      541 tcaaggtcga agattttgag caatccattg cgcggaccaa tcagcgtgag caaccgcgtc
      601 cggtgtcact ctattttcac cttccgtttt gtgagtcgtt gtgtttgtac tgtgcctgca
      661 cgacggtcat caccaaaaat catgctgtgg tcgaacccta tctggcgcaa atcaagcgcg
      721 aagtgggctg ggtgagcgag cacctggatg ccggacgcca ggttgagcag attcactttg
      781 gtggagggac gccaacgttt ttaactccgg atcagttggc tgatctctac ggggcgattt
      841 ccagccagta ccggatttca ccgaccgccg aaatcagcat cgaaattgac ccacgggtga
      901 catctcctga acattgccgg acgctccgtg aattaggctt taatcgggtg agtctgggcg
      961 tgcaggattt cagtcccgaa gtccagaaaa cagtccatcg gattcaatct tatgccatgg
     1021 tcaagcagtt gaacgacacc tgccgtgacc ttgggtttga cagcatcaac attgatttaa
     1081 tttatggcct gccgcaccag acgctcgaaa gctttttggc gacggttgat caagtgattg
     1141 aaattcaacc agatcggatt gcgctcttca gttatgccca tgtgccctgg atgaagcgcc
     1201 agcaaaaaat tctggaaaag catctgccgc caggcgaagt caaatttgag atttttaccc
     1261 aggccatcgc caaattgact ggagccgggt atcgctatgt tggcatggac cactttgcgc
     1321 tggaaaaaga cgaattatgc cgtgcccagg atgaccggac gctccatcga aatttccagg
     1381 gctacacgac caaggcaaat tgtgatttgt ttggttttgg aatgagttca atcagtgggt
     1441 tggaagacac ttacgcccag cactggcata cgctgccgaa ttactatgaa accgctcaca
     1501 aagaagcctg gccattgatg cgcggatgcc agttgaccgc tgacgataag ctccgccggg
     1561 ccgtcatcag ccgcatttta tgccacacgc tgctggtgaa gtcggaaatt gaacgagagt
     1621 ttggaatttg ctttgatcgt tattttgctc ctgaactcga aacattgacc gaactcgaac
     1681 aagatcaact ggttacagta tcatctgatc gaatcgaagc gacgccgctg gggcgaattt
     1741 tgatccggaa cattgccatg atctttgacg cctacttgaa gaaaccggtt gaacaacggt
     1801 tttcgaagac gctgtaagac cagggctgaa gaaatcgggc tgaagaagtc gggct
//
LOCUS       DAOQDO010001427         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64766_length_1854_cov_2.873263, whole genome shotgun sequence.
ACCESSION   DAOQDO010001427 DAOQDO010000000
VERSION     DAOQDO010001427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64766_length_1854_cov_2.873263"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..>1854
                     /locus_tag="PKZ53_19455"
     CDS             203..>1854
                     /locus_tag="PKZ53_19455"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNJ42673.1"
                     /translation="MMSYPEVLIVGAGVAGMTVAYELTRRGHLVTVVEFGPVPDPKTE
                     FLTETWPDELPERGLGRGGAEYYPTAHFTSRRNAPYVIGTEVANSWLKRTRVVGGKTL
                     YWTGHALRFSDVEFQAVSTTGQGENWPITSAELTPFYKRAEQLMGVCGSFEGLSHQPD
                     GEFLPPLELRPGERILQKALAAHHIPLIPARKAILTRNFGHRPACHFAGRCYLGCRTG
                     AKFDAYTGLTQAALQTGKLTLRPDSVVTEVLSNPAGQAMGVRLINRKTRKVEVLNAKI
                     VVLAASAIETARILLNSKSPGKRYSLANQSGLIGHYLTESIGVLVEGLLPQLKGTPVG
                     DITSTGEHGLIPRWVNLSRTSQESYSDGFLFLVESGPGSFPTYAAQLPGFGKSLKQAI
                     QDWYPAAIRLYGIAPVTARRDNRVIVDPRHRDEWGIPTAKVVFSLSEEDGLRWQAMTA
                     SAQEFLELAGAEYITVRQKGPETGGGLHAAGTCRMGNDPKTSVVTSFGQCHDVPNLFV
                     ADAATFVTSLNQPTLTVMALALRQAEFIGNQVPGSGFRVPGSG"
BASE COUNT          405 a          474 c          506 g          469 t
ORIGIN      
        1 ccttatcata agtttcctga gatattgggt ttggtaagtg cttgattttt tccgtatttt
       61 ccgtgttttc cgtggttcaa aatgtctgga aattttcggt aaggtactta tcaagtaata
      121 agacacctta aattgcacca ttaaaataac ttacctatat tttcttcatt cttcattctc
      181 aattcttcat tcttcattct tcatgatgtc ctacccagag gttctcattg tcggcgccgg
      241 agttgctgga atgaccgttg cctacgaact gacgcggcgc ggacatttgg tgacagtggt
      301 ggagtttggt ccggttcccg atccaaaaac cgaatttctg actgaaacct ggccagatga
      361 acttccagag cgtggccttg ggcgtggcgg ggctgaatat tatccaactg ctcattttac
      421 ttcccggcgc aatgcgcctt atgtcatcgg gaccgaagtt gccaatagct ggctcaaacg
      481 cacccgggtg gtcggtggaa aaacgctcta ttggaccggg catgccttgc ggtttagcga
      541 tgttgagttt caggcggttt cgaccactgg ccagggggaa aactggccaa tcaccagcgc
      601 cgaactcaca cccttttaca aaagggctga gcagttgatg ggggtgtgtg gtagtttcga
      661 aggtctatcc caccagccag atggtgaatt tttgccgccg cttgaactcc gaccagggga
      721 aagaatcctt caaaaagcac tggccgcaca ccacattcct ttgattccag cgcgcaaagc
      781 cattctgact cgaaattttg gacatcgccc ggcgtgccat tttgctggcc ggtgttacct
      841 tggctgtcgc accggagcca aattcgatgc ctataccggg ctgacccagg cagctttaca
      901 aaccggaaag ttgacgctcc gacctgattc agttgtgacc gaagtgcttt ccaacccggc
      961 tgggcaggcc atgggcgtgc gattgatcaa ccggaagacc cgcaaagtcg aagtcctcaa
     1021 tgccaaaatc gtagtgctgg ccgcgtcagc gattgaaaca gcgcggattc tgctcaattc
     1081 aaaatcacct ggaaaacggt acagcctggc gaatcaaagc ggtttgattg gtcattacct
     1141 cactgaaagt attggtgttt tggttgaagg cttgctcccg caactcaaag gaaccccggt
     1201 cggtgacatc acaagcactg gcgaacatgg gttgattcca cgctgggtta atctttcgcg
     1261 gacatctcag gaaagttatt cggatggttt tttgtttctg gtggaaagcg gaccggggag
     1321 tttccccacc tatgcagcac agcttcctgg ctttggaaaa tcactgaaac aggcgattca
     1381 ggactggtat ccggcagcga ttcggttgta tggcatcgcg ccagtcactg cccggcgtga
     1441 caaccgggtt atcgttgacc ccaggcaccg ggatgagtgg ggcattccaa ccgccaaagt
     1501 tgtgttttct ctcagcgaag aagacgggtt gcgctggcag gcgatgacgg cttctgccca
     1561 ggagtttttg gaactggccg gtgccgagta cattaccgtc cggcaaaaag gaccggaaac
     1621 tggcggggga ctccacgctg ccggtacctg ccggatgggc aatgacccaa aaacatcagt
     1681 cgtaacctca tttggtcagt gccatgacgt cccgaacctg tttgtggcgg atgctgcgac
     1741 ctttgtgacc tcgctcaatc agccgacctt gacggtgatg gcgctggcgc tccggcaggc
     1801 tgagtttatt gggaatcagg ttccgggttc cgggttccgg gttccgggtt ccgg
//
LOCUS       DAOQDO010001428         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64773_length_1854_cov_2.693163, whole genome shotgun sequence.
ACCESSION   DAOQDO010001428 DAOQDO010000000
VERSION     DAOQDO010001428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64773_length_1854_cov_2.693163"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1517)
                     /locus_tag="PKZ53_19460"
     CDS             complement(84..1517)
                     /locus_tag="PKZ53_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNJ42674.1"
                     /translation="MKFDSYDVGGFYDEMFGPEGEPRPGTRLLAQRLESIPETEILQR
                     QRAAEAALLKLGITFNVYGNSDGIEKIFPFDIIPRIVEAHEWTPIETGLKQRIQALNL
                     FIDDVYHDQKIIRDGVVPEHIITSSKCFLRPCMGLNPPHGVWCHITGTDLVRDDQGQF
                     FVLEDNLRCPSGVSYVLENRVVMKRTFPHVFEDSRVRPVNQYPSKLLETLERLAPQHV
                     SDPTVVLLTPGVYNSAYFEHSFLAQQMGVQLVEGRDLVVEDKYVYMRTTKGFRRVDVI
                     YRRIDDDFLDPQKFRPDSMLGVPGLMDVYRSGRVALANAPGTGVADDKVIYAYVPAII
                     KYYLDQDPILPNVPTYLCSDNQQLQHVLANLDKLVVKTANGSGGYGMLVGPCASAAER
                     AAFADAVRANPREYIAQPTLQLSRVPTLIGNHLEGRHVDLRPYVLYGEEIYVLPGGLT
                     RVALTKGSLVVNSSQGGGSKDTWVLSE"
BASE COUNT          432 a          486 c          498 g          438 t
ORIGIN      
        1 aaaaacagac tcgcaggaga ttgttcagct ctgagcttgc gaatcttcag ccctcagccc
       61 caggcccaag ttcttcaggc tcactattcg gacaagaccc acgtatcctt gctcccaccg
      121 ccctgtgacg aattgaccac cagcgaacct ttggtcaagg ccactcgcgt caacccgcct
      181 ggaagcacgt aaatctcttc tccataaagc acatacggac gcaggtccac gtgacggcct
      241 tcgaggtgat tcccaatcaa ggttggcacc cgtgaaagct ggagcgtcgg ttgggcaatg
      301 tattcgcgcg ggttagcacg cacagcatcg gcaaatgcag cgcgttctgc cgcgctggca
      361 cagggaccga ccagcatgcc gtagcctcct gaaccattgg ctgttttcac caccagtttg
      421 tcaagattgg ccagcacgtg ctgcaattgc tggttgtcac tgcacagata ggttggaaca
      481 ttgggtaaaa tcgggtcctg gtcaaggtaa tacttgatga tggctggaac gtaagcatag
      541 atgaccttgt cgtcagccac acccgtgccc ggagcgttgg ccagtgcgac ccgcccggag
      601 cggtaaacat ccatcaaacc tggcacaccc agcattgaat ccggtcgaaa tttctgtgga
      661 tccagaaaat catcgtcaat ccggcgataa atgacatcta cccggcgaaa ccctttggtt
      721 gtccgcatat agacatattt atcttcgacg acgaggtcac ggccttcgac cagttggacg
      781 cccatttgct gggcgaggaa ggaatgttcg aaataggctg agttgtacac cccaggcgtg
      841 agcagcacca ccgttgggtc agacacgtgc tggggggcca gtcgctccag cgtctcaagt
      901 aatttgctcg ggtactggtt gaccgggcgc acccgcgaat cttcaaacac gtgcggaaac
      961 gtgcgtttca tcaccacccg gttttcgagc acatatgaca cccccgacgg acagcggagg
     1021 ttgtcttcga gcacgaaaaa ttgaccttga tcatcgcgca ccaggtcggt tccagtgata
     1081 tggcaccaca cgccatgagg tgggttcagc cccatgcagg gtcgcaaaaa gcatttactc
     1141 gacgtgatga tgtgttccgg aaccacgccg tcacggatga ttttctggtc gtggtacacg
     1201 tcgtcaataa acagattcag tgcctgaatt cgctgtttca gtcccgtttc gattggcgtc
     1261 cattcgtggg cttcgacaat gcgggggata atgtcaaacg ggaaaatttt ctcgatcccg
     1321 tctgaattgc catagacgtt gaaggtaatc ccaagtttga gcagggcggc ttcggcggct
     1381 cgctggcgct gtaaaatctc ggtttccggg atggattcga gccgttgcgc gagcagtcgc
     1441 gtccccggac gcggctcacc ttcgggtcca aacatttcgt catagaaccc gcctacgtcg
     1501 taggagtcga atttcatacg acctctgtgc tggattgtag aatgtgctgt aattgtggag
     1561 ggggaaaaaa gatgatgtat gctagcggtt cagccgagaa aatgaaactc caaatttttt
     1621 ccaaacggtt tgttacagtc aaacccagat gcctgccacc caggtatttc aagctggttg
     1681 tgatgttttt gggcagtctg aagttagaac aaagagtctc gtaacttttg ttactgcttg
     1741 atatgtctgc ttttaagaag gggaacccta tggcggaagc tgaaaaagaa ctcgtgattt
     1801 caccacggcg aaaaatcctg cggttttgcc tcgtgagctt gattgtggtg gtgt
//
LOCUS       DAOQDO010001429         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_64910_length_1852_cov_2.050640, whole genome shotgun sequence.
ACCESSION   DAOQDO010001429 DAOQDO010000000
VERSION     DAOQDO010001429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64910_length_1852_cov_2.050640"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1633)
                     /locus_tag="PKZ53_19465"
     CDS             complement(218..1633)
                     /locus_tag="PKZ53_19465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNJ42675.1"
                     /translation="MSTDVVMPQMGESIAEGTIVKWIKKIGDTVERDEPIFEISTDKV
                     DAEIPSPVAGVLLEILVTEGQTVPINTVVARLGDASEVGAAAPAAAPAPEPVVAAEPA
                     PVVVEAPPAPVVAEAPPAPVVEPVEEEEDEASAEEQRKVKSSPLVRKIAREHGVDLTQ
                     VTGTGLGGRVSKEDILAFVESRKAPVAAPPAPAPVVVAPPPVVVAPPPPVVVAPPPPV
                     VVAPPPAAPAVVSTPTAYGASDRVEIVPMSPMRKKIAEHMIHSRRTSAHVQSFFEVDM
                     TNVVKLRERNKDKFEQRNGTKLTMTTFIVKAAVEGLKAFPIMNSSVDGDYIIYKKDIN
                     IGVAVALDWGLIVPVIKHAEEKNLLGIARTLNDLANRARNKQLKPDEVAGGTFTITNP
                     GIYGSLMGAPIINQPQVAIMGVGAINKRPVVVNDDAIVIRSMAYFSISFDHRVIDGAV
                     ADQFMAHVKKTLENFQTTELE"
BASE COUNT          401 a          514 c          491 g          446 t
ORIGIN      
        1 aggtatgaaa cccgttctct tcagccccga gcttgtgagt cttcagcccc aagccctaaa
       61 accagggttg aagacttcgg gctgaagaag tcgggctgaa gaagttgggc tcagggtatg
      121 aaacccgttt tcttcagccc caggcccgat gttttcagcc cgaagtcttc agccccaggt
      181 ctttcagccc gaagtctttc ggcctgctgg ctcaatgtta ctcaagctca gttgtctgga
      241 aattttccag cgtctttttg acgtgggcca taaattgatc ggccacggcg ccatcaatca
      301 cccggtgatc aaacgaaatt gagaaatagg ccatggaccg aatcacaatt gcgtcgtcgt
      361 tgaccaccac tggacgtttg ttgatggcac caacgcccat gattgccacc tgcggctgat
      421 tgataattgg tgcgcccatc aaacttccat agattccagg attggtgatg gtaaaggtgc
      481 cgccagcgac ttcatccggc ttgagttgtt tgttgcgggc ccgattggcc agatcattga
      541 gggtgcgggc aatgccgagc aggtttttct cttcggcatg tttgatcact ggaacaatca
      601 acccccaatc caacgcaacg gccacgccga tgttgatgtc cttcttatag atgatgtaat
      661 cgccgtcaac cgacgaattc ataatcggga aggctttgag cccctcgacc gcggctttga
      721 caataaaggt cgtcatggtg agcttggtgc cattgcgctg ctcaaacttg tctttattgc
      781 gttcgcggag tttcacgaca ttcgtcatgt caacttcaaa gaatgactga acgtgagctg
      841 aagtccgccg actgtggatc atatgttcgg cgatcttctt gcgcatcggt gacattggca
      901 caatttcaac ccggtcactg gcgccatagg ccgttggagt agaaactaca gctggagccg
      961 ccggcggcgg ggctaccaca actggaggtg gcggggccac aacgaccggc ggcggtggcg
     1021 ctaccacaac tggtggcggg gccaccacaa ctggagctgg ggcaggaggt gccgcaaccg
     1081 gggctttccg gctttcaaca aaggccagaa tgtcttcttt tgatacgcga ccacccaaac
     1141 cggtgccggt gacctgggtt aaatcaacac cgtgttcgcg agcaattttg cgaaccagcg
     1201 gagaagattt cactttgcgc tgctcttcgg ctgaggcctc atcttcttcc tcttcaaccg
     1261 gttcaaccac cggggcgggc ggcgcttcag ccacgaccgg tgcaggtggg gcttcgacca
     1321 caactggtgc aggttcagca gcgaccactg gttccggtgc gggtgctgcg gctggggccg
     1381 ccgcgccaac ttcagaggca tcaccgagtc gggcgacaac ggtgttaatc ggtaccgtct
     1441 gaccttcggt caccagaatt tccagcagca caccagccac cggtgatgga atttcagcat
     1501 caactttgtc ggttgaaatt tcaaaaattg gttcatcgcg ctcgacggta tcgccgatct
     1561 ttttaatcca cttcacaatc gtgccttcag cgatggattc ccccatctga ggcatcacga
     1621 catcagtact cattgcggtt cacctttcta caacgatgat ttaaaacaga cgtggataaa
     1681 ggcttgaggg cgtacaggag tctcagtgtc tggcacctga tacgttatgc tttagtctca
     1741 ggcttctaga atacattgcc agctatcagt caccaggcac tcagtaccag ccctcaaccc
     1801 tcgttttttt gcctagtaag ctgcaagctt tcgggccgcg gccaccactt tt
//
LOCUS       DAOQDO010001430         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65004_length_1850_cov_3.449582, whole genome shotgun sequence.
ACCESSION   DAOQDO010001430 DAOQDO010000000
VERSION     DAOQDO010001430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65004_length_1850_cov_3.449582"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..969
                     /locus_tag="PKZ53_19470"
     CDS             205..969
                     /locus_tag="PKZ53_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNJ42676.1"
                     /translation="MIKALLARFQQGHRTMSFPDGPPPVLPGRFRGRPVLETTKCPDG
                     CRKCADACPTAAITIADKARIDLGRCLFCTDCQDACPCQAVNYLQDYRLATRSRNDLM
                     VAQDEELVLAKALDAKLQRLFGRSLKLRQVSAGGCNACEADVNVLGTVGWDIGRFGIQ
                     MVASPRHADGLLITGCVTENMKLALEKTYAAVAAPKLVIAVGACAISGGPFLDLPAQH
                     NGAASVVPVDLYIPGCPPHPLTILDGLLRLLGRLEE"
     gene            1073..1747
                     /locus_tag="PKZ53_19475"
     CDS             1073..1747
                     /locus_tag="PKZ53_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ42677.1"
                     /translation="MSRIRVLIVDDHAILRAGLRMLISAQPDMDVVDEAGNGQEAIQK
                     TQALKPDVVLLDITMPGINGLKAIEEILQMCPKTKVLVLTMHEDPAYLKSALAAGSSG
                     YVVKRAADSELLAAIRTVYRGKVFIDRTLGDDLIQDLIGKKPHDPHKNADAFHHLLSK
                     REREVLQLLAQGYTNQQVAASLSVSIKTAETYRARVFQKLGFRDRVDLVRYAVEIGIL
                     TPDKLT"
BASE COUNT          454 a          473 c          501 g          422 t
ORIGIN      
        1 gaattggggc tgaaaatacc agggctgagg gctgagggct gaagtctcgc aagctcgggg
       61 caatcgcagg gatgagggat gaaagatgag ggatgaaatc aaaccacttc ttcagccctc
      121 agccccaagc ccgagttctt cagcccgttg tcttcaaccc caagccctta gccctaagcc
      181 ctcagcccta agccctaaaa tgatatgatc aaagcacttc tcgctcgatt tcaacaggga
      241 caccgcacga tgtccttccc agatggccca ccgccagtct tacccggacg ctttcggggg
      301 cgtccagtcc tggagaccac caaatgtcct gatggctgtc ggaagtgtgc tgatgcctgc
      361 ccaacagccg ccattaccat tgcggataaa gcccggattg acctgggaag atgccttttt
      421 tgtaccgatt gtcaggatgc ctgtccttgt caggctgtga attatcttca agattatcgg
      481 ctggcgactc gctcacgaaa tgatctgatg gttgctcaag atgaagaact ggtgctggcg
      541 aaggcactcg atgcgaaact ccagcggctg tttggacgct cgttgaagct ccggcaggtc
      601 agcgccggtg gctgcaacgc ctgcgaagcc gatgtgaatg tactgggaac tgtcggctgg
      661 gacatcgggc ggtttggaat tcagatggtg gcatcccctc gccacgccga tgggttgctc
      721 attactggat gcgtcaccga gaatatgaaa ctggcgttgg aaaaaaccta cgctgccgtc
      781 gccgctccaa aactcgtgat tgcggttggt gcctgtgcca tttccggcgg accatttctc
      841 gacttgcccg cccaacacaa tggggcagca tccgtcgttc cggttgattt gtacattcca
      901 ggatgcccac cacatccgtt gaccattctg gatggattgt tgcggttgct gggaagactg
      961 gaagaatagg ggtcaggggt caggggttga aagacaaaaa ggacaaaaaa acataaattc
     1021 aaaaccctga accctgaacc ctgaaccctg taaaaaagct gggagccatc aaatgtccag
     1081 gattcgtgtt ttgattgttg atgatcatgc cattctcaga gccggtctcc ggatgctcat
     1141 cagcgcccag ccggatatgg acgtggttga cgaagccgga aatggtcagg aagcaatcca
     1201 aaaaacccag gcgttgaaac cggatgtcgt gttgcttgac atcaccatgc cgggaatcaa
     1261 cgggctcaag gcgattgagg aaatcctcca gatgtgtccc aaaaccaaag tgctggtttt
     1321 gaccatgcac gaagacccgg cttacctgaa atcagcgctg gcggcgggga gttcaggata
     1381 tgtcgtcaaa cgggcggctg attcagagtt gctggctgcg attcgcacgg tgtaccgggg
     1441 aaaggtgttt attgatcgca cgctgggtga tgatttgatc caggatttga ttgggaaaaa
     1501 accccatgac cctcacaaaa acgccgatgc ctttcaccat cttctcagca aacgcgaacg
     1561 cgaagtcctg caacttctcg ctcaggggta caccaatcag caggtggcgg cgtcactgag
     1621 cgtcagcatc aaaacggctg aaacctatcg ggcacgggta tttcaaaaac tcggatttcg
     1681 agaccgggtg gatctggtcc gttatgcggt ggaaatcggt attttaactc ctgacaaatt
     1741 gacttaaagc caggtaggca actcgtcttc attcaattat cggttgaaat catgattggc
     1801 aaccgttttc ttttttggcc caaaccagaa caatctgata ttgttcaaat
//
LOCUS       DAOQDO010001431         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65224_length_1847_cov_3.157366, whole genome shotgun sequence.
ACCESSION   DAOQDO010001431 DAOQDO010000000
VERSION     DAOQDO010001431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65224_length_1847_cov_3.157366"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PKZ53_19480"
     CDS             complement(<1..1662)
                     /locus_tag="PKZ53_19480"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNJ42678.1"
                     /translation="MRQKQIRNNYLTKLICTSLLAVMSGWSDPIWGQNIRSTIPNSAL
                     QSSPTEISQDFSGSFPTHVQVARPGSGTESQRITRIDLEVRFSNGSPATDTGLRDLLD
                     LRVGEEYSPVKINQALRRIYQSRRASNAQVFLTGGESPGSVVLRFVITRQERVKDIFF
                     SGNQFFSPEELRARLVGLDVNAPLSNRAVEEGQRTLLGVFQEKGFFRAKIDWTISDPD
                     VDGRVDITYRIQQGDQALLAKPIQIEGNIKIDLKKLTEKLKGKPGIPYTTDLIQSDLE
                     KIRQWHLDASYLSPQISLPFAEYDPATNTVTVSANLDSGPLVDLKIEGYDLSRKEQRD
                     ILPILRYGGLDDFVLEDGKRKMLTYLQTQGYFFAAVDYTKSVFEETASVVYTVEKDRR
                     YRLEAVKLIGTDKFTILDIGPELLSQPAGILPRSRGKTSLESLTTDAGLIERKLRNLG
                     YLQARVTERRLGISPDKPDFEVTFVVDEGQRTLIKEVAFLGNDTFTADRLRMEILAHA
                     PDDNYFSQERLNQDINQLYSLYEEAGYARAVITPKIEFADATPNEA"
BASE COUNT          433 a          445 c          475 g          494 t
ORIGIN      
        1 ggcttcgttg ggggtagcgt cggcaaactc aattttgggc gtaataaccg cccgcgcata
       61 tccggcttct tcatacaggc tgtagagttg attgatgtcc tggttgagtc gttcctgcga
      121 aaagtaattg tcatctgggg catgggccag gatttccatc cgtaaccggt cggcggtaaa
      181 ggtatcgttg ccaagaaagg cgacttcttt gatcagggtc cgctggcctt catcaaccac
      241 aaaggtcact tcaaaatcag gtttatcggg tgagatcccc agtcgccgtt cggttacgcg
      301 tgcctgtaag tatccaaggt tcctgagctt gcgttcaatc aaccctgcat cggttgtcag
      361 gctttcaaga ctggtttttc cacgtgaccg gggaaggatt ccggctggct gggaaagcag
      421 ttcaggacca atatcaagta ttgtaaactt atcagttcca atgagtttga cggcttccag
      481 tcggtaccgc cgatcctttt ccacggtgta caccaccgaa gccgtttctt caaaaactga
      541 cttggtatag tcaactgccg caaagaaata tccctgggtt tgtaaatagg tcagcatttt
      601 tcgctttcca tcttccagga caaaatcatc aagcccgcca taccgcaaaa tcggcaggat
      661 atcgcgttgt tctttgcgcg agaggtcata tccttcaatt ttgagatcaa ccagcgggcc
      721 agagtccaga ttggctgaca cggtgaccgt attggttgcc gggtcatatt cggcaaaggg
      781 aagcgagatt tgggggctca ggtaactggc atcgaggtgc cactggcgaa ttttctccag
      841 gtcggactga attaaatcag tggtataggg aattccgggc tttcctttca atttctcagt
      901 cagtttcttg agatcaattt tgatattgcc ttcaatctga attggtttgg ccagcagggc
      961 ctgatcgcct tgctgaatgc gataggtgat gtcaacccgc ccatcaacat ctgggtcgct
     1021 gatcgtccag tcaattttgg ctcgaaaaaa ccctttttcc tgaaagacac ccagtaaggt
     1081 tcgttgtcct tcttcgacgg cccgatttga caggggagca ttgacatcga gcccgaccag
     1141 ccgggcgcga agctcttcgg gtgagaagaa ctgatttcca ctaaagaaaa tgtcctttac
     1201 ccgctcctgt cgggtaatta caaatctaag caccactgat ccaggacttt ccccgccagt
     1261 caggaagacc tgggcattgg aggcccggcg cgactggtag attcggcgca gcgcctgatt
     1321 gattttgact ggggaatatt cttcgccaac ccggaggtcg agcaggtcgc gcaagccggt
     1381 atctgtggct ggcgacccat ttgaaaagcg aacttcgagg tcaatccggg taatccgctg
     1441 tgactcagtt ccgcttccag gtcgagccac ttgcacatgg gttgggaaag agccgctgaa
     1501 atcttgagaa atctcggtgg gtgatgattg aagggctgaa ttgggaatgg tgctcctgat
     1561 attttgtccc caaatgggat cgctccaccc gctcatcacg gccagcaagg aggtacagat
     1621 caacttcgtt agatagttgt ttcgaatctg tttctgtctc atgaaatgat tgccctggct
     1681 ctgtgaaatt gaagaattga gaatgaagaa ttgaaaaaac ggttggttct tggttcttgg
     1741 aaagtattgg ttcctaagga ctaagcacta agtaccaggc acgttctcaa ttctcaattc
     1801 tcaattcttc attcctttta aaaccgtttg gtaaaccgca cgtccac
//
LOCUS       DAOQDO010001432         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65252_length_1847_cov_2.204241, whole genome shotgun sequence.
ACCESSION   DAOQDO010001432 DAOQDO010000000
VERSION     DAOQDO010001432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65252_length_1847_cov_2.204241"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKZ53_19485"
     CDS             complement(<1..379)
                     /locus_tag="PKZ53_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HNJ42679.1"
                     /translation="MSENQPPMAPPSLPPNVTLLHLITGRWISQMIYAAAKLGIADLL
                     TDGGKSVEQLAETTQTHPRSLFRLLRSLAAVGVFTESKPHFFELTPLASCLRSDAPDS
                     MRAMAIMFGEDFHYRAWGNILHSI"
     gene            complement(514..>1847)
                     /locus_tag="PKZ53_19490"
     CDS             complement(514..>1847)
                     /locus_tag="PKZ53_19490"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNJ42680.1"
                     /translation="SETEPNDAPANAQVLNGTTITVNGNVNPGDSGGTFIQFGGNTQD
                     RIEDLYRITIPSTMTVALALDWAQTAADLDLYLFNNSLVPGSANAAFVDGSASDTLKP
                     ERIGARILQPGTYYIGVSRFDQSNSPATAYQLRFASQPGGTSQTLSLDDGRAELFFTS
                     GTVIFANRLTPPSYPATLNQVLLSVNNRATNQPASAVGSVVRIIVYSIDSTGARALQG
                     VENFLITNDGTTPDFTANTFNITDVTIQNGSFLVGYQVVQPNASGVSPFATVDSTSPK
                     RNTLISVDNGTTFTELIAQNNQTGNLLLRGEVTLFNNTLQMVSPDSTPFEGLIQNAST
                     QPLQAIEAAPTPPVKQRVGLHRLNLESIATSQEITLELADPVGVSSVEALIKRGENED
                     WIPIEIRRQSFNPVTRLWTITVPNQPGYTAIRYRNQQGWLSNVKEIPPSAN"
BASE COUNT          424 a          409 c          542 g          472 t
ORIGIN      
        1 gaatactgtg caggatattt ccccaggcgc ggtagtggaa atcttcgcca aacatgatcg
       61 ccatggctcg catggaatca ggggcgtctg accgaaggca ggacgcaagc ggcgtcaatt
      121 caaaaaagtg aggtttggat tcggtaaata ctcctacggc agcgagactg cgcaagagtc
      181 gaaacaggga gcgaggatgg gtttgagttg tttcggctaa ttgttcaact gacttgccgc
      241 cgtctgtcag caagtcggca atcccaagtt tggctgccgc atagatcatt tgtgaaatcc
      301 aacgcccggt gatgaggtgc agcaaggtga cgttgggggg aagcgatgga ggggccatcg
      361 gtggttgatt ttcagacata gattctcctg aagaaagata tatgtgaatg ttggctttat
      421 tatgagggcg aacctgagag aggtgtcagc cgtgggacaa tcctgatgcg agttgtgccc
      481 cacggctggg tgctggtgaa ttgagattga agatcaattg gcggaagggg gaatttcctt
      541 gacgttgctg agccatcctt gctgatttcg gtaccggatg gctgtgtatc ctggctggtt
      601 ggggacggta atcgtccaca atcgggtaac tggattaaaa ctttggcggc gaatctcaat
      661 cggaatccag tcttcgtttt caccacgctt gatcaaggct tcaaccgacg aaaccccaac
      721 tggatcagcc agttccagag ttatctcctg gctggttgcg attgattcca gattcaggcg
      781 atggagccca accctttgct tgactggtgg cgttggggcc gcttcgatgg cctggagcgg
      841 ttgcgttgat gcgttctgaa tcagtccctc aaacggggtg gaatcaggtg ataccatctg
      901 gagggtgttg ttaaacaagg tcacttcgcc acgcagtaac aggtttccgg tttgattatt
      961 ttgggcgatc agttctgtaa aggttgtgcc gttatccact gaaataaggg tgtttcgttt
     1021 gggtgatgta ctatccacgg ttgcaaaggg agaaacccca gatgcatttg gctggacaac
     1081 ctgatagcca accagaaagc tcccgttttg aattgtgaca tctgtgatgt tgaaagtatt
     1141 tgccgtgaaa tcgggtgttg tcccatcgtt ggtgatgaga aagttttcaa ctccttgcaa
     1201 tgctcgtgct ccagtggaat caattgaata cacaataatc cgcaccactg atcccaccgc
     1261 agaagccggt tgattcgtgg cccggttatt gaccgaaagc agtacctgat tcaatgttgc
     1321 cgggtaactc ggtggcgtca ggcgattggc aaaaatcacc gtgcctgagg tgaaaaacag
     1381 ttcagcccgg ccatcgtcca ggctcagagt ttgagatgtc ccgccaggtt gactggcaaa
     1441 acgaagctgg taggcggtcg ccgggctatt ggattgatca aaccggctga ccccgatgta
     1501 ataggttcca ggttgcagaa tgcgagcgcc gatgcgttca ggtttcagtg tgtctgaggc
     1561 tgacccatcc acaaaggccg cattggctga ccctggtacc aaagaattgt tgaagagata
     1621 gaggtccaga tccgctgccg tttgtgccca atcaagtgcc agagccactg tcattgtgga
     1681 tgggatggtg atgcgataga ggtcttcaat tcgatcctgg gtgttacccc caaactggat
     1741 aaaggttccg ccgctatcac ctggattgac atttccattg acggtgatgg ttgttccgtt
     1801 gagtacctgg gcattggctg gagcgtcatt tggttcagtt tcagatg
//
LOCUS       DAOQDO010001433         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65288_length_1846_cov_3.017309, whole genome shotgun sequence.
ACCESSION   DAOQDO010001433 DAOQDO010000000
VERSION     DAOQDO010001433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65288_length_1846_cov_3.017309"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKZ53_19495"
     CDS             complement(<1..420)
                     /locus_tag="PKZ53_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42681.1"
                     /translation="MALSTKQGTMMQKTGCVISDGFQLQYIIDGEGLPTIVVGSALYY
                     ERTFSKHLRTQLKMAFLDHRGFAPGNHCTDLEKYRLEVLVDDIEVMRKALGFEQFVLI
                     GHSGHTFMALEYAKKYPANVTHLVLMCSSPDFSEAGRA"
     gene            complement(463..>1846)
                     /locus_tag="PKZ53_19500"
     CDS             complement(463..>1846)
                     /locus_tag="PKZ53_19500"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ42682.1"
                     /translation="AHLPQKTPSKPLVLTHVTIIDPTGGPTKSDMTLIIQGDLISELG
                     KHNSVRIPKDVQVVDATGKFLIPGLWDMHIHWYEPESLSLFVANGVTGARVMWGHSLH
                     HQWRKDIEAGKLIGPRLVIASPIIDGPNPIWPGSVAVNNEAEARQAVKTAKQEGADFI
                     KVYSLLSREAFFAIADEAKKQGIAFAGHIPQSVTVIEAAEAGQKSVEHLTGMLTACSS
                     IEDELRKNEDIAGASSAHGKGTVDPAAIRQLTRSVIESYDAKKAANLFAKLKQHHTWQ
                     CPTLTVLRSIAFLTDPEFRNDPRLKYMPAGMRTRWDPANDFRFKARTAEDYELSKLVL
                     QRQFEIVGQMRKAGVEFLAGTDTLNPYCFPGFSLHDELELMVKAGFTPLEALQSATLN
                     PARYLGQEKNMGTVEKGKLADLVLLDANPLEDIKHTRKINAVVYGGKLYPRAELDRML
                     ATVEAGAKKK"
BASE COUNT          415 a          489 c          466 g          476 t
ORIGIN      
        1 cgcacgtcca gcctccgaaa aatcgggact ggagcacatc aggaccagat gggtcacatt
       61 ggccgggtat tttttggcgt attcgagggc cataaaggtg tgcccggaat gaccaatcag
      121 gacaaattgt tcaaatccaa gcgctttccg catcacctcg atatcgtcaa ccaggacttc
      181 aagccggtat ttttcaaggt cagtgcagtg attgcccggt gcaaagccac gatggtcaag
      241 aaaggccatc ttcaactgag tgcggaggtg ttttgagaac gttcgttcat aatacagcgc
      301 acttccaacc acgatggtcg gaagcccttc accatcaatg atatactgca gttgaaatcc
      361 atctgaaatt acacagccag ttttctgcat catcgttccc tgtttcgttg agagtgccat
      421 tctcttgacg tcgctttgga atttggcggc ttgacgacca gtttattttt tctttgcacc
      481 agcctcaacc gttgccagca ttcggtcaag ttctgcccgt gggtacaatt tgccaccata
      541 caccacggcg ttgatcttcc gggtatgttt gatgtcttca agcgggttgg catccagcaa
      601 aaccagatca gccagttttc ccttttcaac cgtgcccatg tttttttcct gaccaagata
      661 gcgtgccgga ttgagtgttg ccgactgaag tgcctctagc ggtgtgaacc cagccttgac
      721 catcaattca agttcatcgt ggagactaaa cccaggaaaa caatagggat tgagcgtatc
      781 tgtacccgcc agaaattcaa ccccagcctt tcgcatctgg ccgacaattt caaattgacg
      841 ttgcagcacc agttttgaga gttcgtaatc ttcggcggtg cgcgccttga accgaaaatc
      901 attcgccggg tcccatcggg ttctcatgcc agcgggcatg tatttgagcc gtggatcatt
      961 tcgaaattcc gggtcggtca ggaaggcaat gcttcgcaag acggtgagcg tcgggcattg
     1021 ccaggtgtga tgttgcttga gtttggcaaa caagttagcc gcttttttgg catcataact
     1081 ctcaataacc gaacgagtga gctgcctgat tgcagccgga tcgacagttc ctttgccgtg
     1141 cgccgaactg gcaccggcaa tgtcctcatt tttccgcaac tcgtcttcga ttgaagaaca
     1201 cgccgtaagc attccggtca ggtgctcgac acttttttgg ccggcttcag ccgcttcaat
     1261 aactgtcact gattgtggaa tatgcccggc aaaggcaatc ccctgttttt tcgcttcatc
     1321 agcaatcgca aagaaggctt cgcgggaaag caggctgtag accttgatga aatcagcgcc
     1381 ttcctgtttt gccgttttta cggcctgtcg ggcttcagct tcgttattaa ccgcaaccga
     1441 acccggccag atcgggtttg ggccatcaat aatcggactg gcaatgacca ggcgtgggcc
     1501 gatgagtttt cccgcttcga tgtcttttcg ccactggtga tggagcgaat gtccccacat
     1561 cacccgcgca ccagtcactc cgttggcaac aaagagcgac agggactctg gttcatacca
     1621 gtggatgtgc atatcccaga ggccgggaat gagaaacttc cccgtcgcat ccaccacctg
     1681 cacatccttt gggatccgca ccgagttgtg cttccccaac tcggaaatga ggtcaccctg
     1741 gatgatcaac gtcatatccg atttggttgg accacccgtg ggatcgataa tggtgacgtg
     1801 ggtcagaaca agtggtttgg aaggtgtttt ttgcggcaga tgggcc
//
LOCUS       DAOQDO010001434         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65307_length_1846_cov_2.499162, whole genome shotgun sequence.
ACCESSION   DAOQDO010001434 DAOQDO010000000
VERSION     DAOQDO010001434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65307_length_1846_cov_2.499162"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>1846)
                     /locus_tag="PKZ53_19505"
     CDS             complement(106..>1846)
                     /locus_tag="PKZ53_19505"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ42683.1"
                     /translation="LNGRNYATDFDKHLIRTGLFGQGLTNYAGQSGSPGNTDGALNQV
                     AFNGPLGLTVGDSAYIADINNQSVRKIDLTSGQTSTVVTLADVIASFPTVTNWGPRDV
                     EVDSNGDLYISDAVNHVIWQITFAGDAPTLSVLAGSIGQSGTVDGDGTAARFNSPQRL
                     TLEDQILSLTENSNRVRQIALPGGAVKSITSSSSKSVTKQQQPPPPPEEKGKLVLPEA
                     IAADVSGNLYVIDEGTVKLVIINRTTGEPLVNNLAPPGTFVNAVAVSVTDDTVYAVDA
                     GTGMLVRVGSAPPRVTSLSPGEVAVGEERVITVTGANFLRDTQVRVGGQPFTEVNIIS
                     PERLTFKVPPQRSNGSVPVIVYNRAGRAETMLTVTGDSVPFINLAAFPDARLVKPDQR
                     AVYTIKIEKSEVTGPVNLTVNGLPPGARAVFSPNPVSGDETILEISVARETSPGEYKF
                     DVVGTASGVSPAMIPIRLTVETGPAQGGTVVLLANPQTLMVNRDGSGMAIINVQRNNY
                     RGQIDLFARVLEGPSAGLNAQINPQQVNLESSATLMVGTRRETQPGTYRVEISARGQD
                     VTIKPIEVQVVVR"
BASE COUNT          420 a          523 c          477 g          426 t
ORIGIN      
        1 gctgtgtagc ggtggctgaa ttaaggcagg tcgtttacgg cctgtaaact gtaaacaagg
       61 atcgccgttc gaaaaccccg aaccctgaac cctgaaccct aaccatcacc tcaccacaac
      121 ctggacttca atcggcttga tcgtcacatc ctggcctctg gctgagattt caacgcgata
      181 ggtgccaggc tgggtttcac gtcgggtacc gaccatcagc gtcgccgaag attccagatt
      241 cacttgctgt ggattgattt gagcattcag cccggcagat gggccttcga gcacgcgggc
      301 aaaaaggtca atctgacctc gataattatt tcgctggaca ttgataatag ccatcccaga
      361 tccatcacgg ttcaccatca gcgtttgtgg attggccagc aacaccaccg ttccaccctg
      421 ggcaggccct gtttcaaccg tcaatcgaat cggaatcatt gccggagata cacctgaagc
      481 agttccaacc acgtcgaatt tgtattctcc aggtgaggtt tcacgggcaa ccgagatttc
      541 caggatggtt tcgtctcccg agaccgggtt tggggaaaag acggcacgag caccaggcgg
      601 caagccgttt acggtcaaat tgaccgggcc agtcacctcg cttttctcga ttttgatggt
      661 atacaccgcc cgctggtcag gttttaccag tcgtgcatct ggaaaggcgg ccagattgat
      721 aaatggcacc gaatcgcccg tcacagtgag catcgtctca gcccgtccag cccgattgta
      781 aacaatcacc ggtactgacc catttgatcg ctgcggagga actttaaatg tcagacgctc
      841 ggggctgatg atgttgactt cggtaaacgg ctggccgcca acccgaacct gagtgtcacg
      901 caggaaattg gcgccagtca ccgtaatcac ccgctcttca cctaccgcaa cttcgcctgg
      961 agaaagtgag gtcactcgcg gaggcgctga accaacccga accagcatcc ccgtgcccgc
     1021 gtctacggca tagacagtgt catccgtcac tgaaaccgcc acggcattga cgaacgttcc
     1081 tggcggtgcc aggttgttga ccagcggttc acccgtagtc cggttgataa tcaccagttt
     1141 gacggtgcct tcgtcaatca cgtacaaatt tccgctcaca tccgcggcaa tcgcttccgg
     1201 caaaaccagt tttcctttct cttccggagg cggaggcggt tgttgctgtt tggtcacgct
     1261 tttcgacgac gacgaagtaa ttgatttgac ggcccctccg ggtaaggcta tctgacgaac
     1321 acggttgcta ttttcggtca aactgaggat ttgatcttcg agcgtgagcc gttgaggcga
     1381 gttaaaccgg gctgctgtgc cgtctccatc aacagttcca gattggccga tacttccagc
     1441 cagaacgctc aatgtcggcg cgtcacccgc gaaagttatc tgccagatga catggttgac
     1501 ggcatctgaa atgtagagat ccccattgga atcaacttcg acatcgcgcg gcccccagtt
     1561 ggtcaccgtg ggaaacgaag caatcacatc ggctaaggtc accacggttg aagtttgccc
     1621 ggaggtcagg tcaattttgc gcactgactg gttgttgatg tcggcaatgt aggcgctgtc
     1681 accaacagtc agcccaagcg gtccgttaaa agcaacttga tttaaagcgc catccgtgtt
     1741 tcccggtgaa cccgactggc cggcatagtt ggtcaacccc tgtccgaaca agccggtccg
     1801 gatcaaatgt ttatcaaagt cagtggcgta gtttctccca tttaga
//
LOCUS       DAOQDO010001435         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65318_length_1846_cov_2.371301, whole genome shotgun sequence.
ACCESSION   DAOQDO010001435 DAOQDO010000000
VERSION     DAOQDO010001435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65318_length_1846_cov_2.371301"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..1399
                     /locus_tag="PKZ53_19510"
     CDS             392..1399
                     /locus_tag="PKZ53_19510"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNJ42684.1"
                     /translation="MAHHVFQHTREHQRHLERIRRLDEWFRSGQFFTIRWLCEQLERS
                     EKTVKRDLEELRNTHYRPLAYCARERAWHYTAPVSPLLPVSLSEGELVTILMAEQMAR
                     TYAGNTLGGQLSVAFSKILSVLTEAVSIELSQLAMIQSVDLLPTTDIDFALFETLMRA
                     IHTRTRLEMTYFTQSSGKVSNRRVDPLHLHSSKGEWYLIAFDHNRNKVLDFHLGRVRE
                     YSLTSEHFEVPETFDLHAVLDSGFGMFRGSGDRLYDVVVEFDAYQAGWIAQQSKVHRT
                     AEYTPLDGGGLRVTMRVGALEGVKMWLLQYGAHVKVIEPPELRQILKTEIEKMKELYE
                     D"
     gene            1606..>1846
                     /locus_tag="PKZ53_19515"
     CDS             1606..>1846
                     /locus_tag="PKZ53_19515"
                     /inference="COORDINATES: protein motif:HMM:NF021100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HNJ42685.1"
                     /translation="MLIDACFRLIGSEIPIDHGYALYSCLSRVLPFLHTSDSIGIHPI
                     SGVPSGKGRLLLTEHSRLSLRIPVESFREVLPLAGQ"
BASE COUNT          467 a          447 c          453 g          479 t
ORIGIN      
        1 ctcgcaaccg accggctatc cgaacggcag cctttcagga tgctcaatcc aaatgtctgc
       61 cctcaaacga aaacgggaag ttaaatttta acgcttccta agtttatttg attgaactat
      121 ttgactattt cctgacacga ggattccatt ccaaaatggt ataattgggc agcagactgc
      181 tgaccaattt tgaggtccgg gcatatttgg gcgaaatact tcatgtattt caggttatac
      241 ctggtcggaa tcgccagctt tgtggcccgc aattgaaaga ttttccgcta ctataagtag
      301 agggatgaga aagccgcagt tggaaacaac ttgaaatatt tccggagggc acgcctggta
      361 ggtcattcct tgacctacct ctggttgtac gatggcacat cacgtatttc aacacacccg
      421 tgagcatcaa cgtcacctcg aacgcattcg ccgcctggac gaatggtttc ggagtggcca
      481 atttttcacc atccgctggt tgtgcgaaca gcttgaacgc tcagaaaaaa ccgtgaaacg
      541 cgacctggaa gaactccgca acacgcacta tcgtccgctg gcctactgtg cgcgcgagcg
      601 ggcgtggcac tatacggcgc cagtgagtcc gctcttgccg gtttcgttgt cggagggcga
      661 actggtcacc attctgatgg ctgaacagat ggcccggacc tacgccggaa acacccttgg
      721 cgggcagctt tcggtggctt tttcaaagat tctctcagtt ttaaccgaag cggtttcgat
      781 tgaactctcg caactggcca tgatccagtc agttgacctg ttgccgacga ccgacattga
      841 ttttgcattg tttgagacat tgatgagggc cattcatacc cgaacgcgcc ttgagatgac
      901 ctactttacc caatcgtcag gcaaggtttc gaaccgtcgc gtagacccac tccacctgca
      961 cagttcaaaa ggcgaatggt acctgattgc ttttgaccat aaccgtaaca aagtgctgga
     1021 ctttcacctg ggtcgggtcc gtgaatattc gctgacgagt gaacactttg aagttccgga
     1081 aacctttgat ttacacgcag ttcttgattc tggatttggg atgtttcgcg gcagcggaga
     1141 ccggttgtat gacgtggttg tcgaatttga cgcctatcag gccggctgga tcgcgcagca
     1201 aagcaaagtc catcgcacgg ctgaatatac accgctcgac gggggcggcc ttcgagtgac
     1261 aatgcgagtc ggcgccctgg aaggcgtgaa aatgtggctg ctgcagtacg gcgcccacgt
     1321 caaagtcatt gaaccacctg aactgcgcca aatactgaaa actgaaattg aaaaaatgaa
     1381 agaactttat gaagattgag gaaaacgcct tacggcaatc gaagatagag gttaaatacc
     1441 tctttcaaat agctaaaact acatcacaat ctcacttgct caacgccttt cggcagtttg
     1501 aagaagttct gagaaacata ttgacaacca gcctaagaat caagctatag ttttttcaga
     1561 tggtgagatt gtgacgtagt aaagtaaact tcaggagaat aaactatgtt gattgatgcc
     1621 tgtttccggc ttatcggttc agagattcca attgaccatg gatatgcgct ttatagttgc
     1681 ctctctaggg tcttaccatt tctgcataca tctgattcta ttgggattca cccaatttca
     1741 ggcgtgccgt cagggaaagg gcggttgttg ctcacagagc actctcgtct gagtttgcga
     1801 attccagttg agtcttttcg agaagtgctt cctcttgctg gccaaa
//
LOCUS       DAOQDO010001436         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65417_length_1844_cov_4.005590, whole genome shotgun sequence.
ACCESSION   DAOQDO010001436 DAOQDO010000000
VERSION     DAOQDO010001436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65417_length_1844_cov_4.005590"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PKZ53_19520"
     CDS             complement(<1..1247)
                     /locus_tag="PKZ53_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42686.1"
                     /translation="MPQRPFAHHVIICLWLGIWGLICLPGMVTAQRISPRPAVAEKTD
                     NAVLNNAREDWVYQQFTDQNGLPQNSVEAICEWNQYVWVGTQDGIACYNGRQWQRVQF
                     PKNILSTFVYALLPASDKSLWVGLNGGGIAHFKHGEWELFSTQTGHLPGNFIRTLYET
                     QEPDGTSTIWAGTVGNGIVRYHQGQWTQVDMRPWLPTNNIRCFLETQTGTTRRLWVGS
                     LGGGLAYLEEGAWHRFEPVAGKLPDTTVHCLVETSETAGQPRLWIGMESGLACLQNGR
                     LEKVLIPSSSRPNSPVIALRETRSHRGEPRLWVATFSGIHCLFQNQWRQVGNIADNRQ
                     QVAISLLATDGINGESSIWVGFNSAGVSRIQKSSWAKFGLDWKPANNTITSLLETIDM
                     DGTPQIWMGTNGSGLVRYRRGEWT"
     gene            complement(1286..1723)
                     /locus_tag="PKZ53_19525"
     CDS             complement(1286..1723)
                     /locus_tag="PKZ53_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42687.1"
                     /translation="MAANFDQPLAELKRKVQTAQNLKEPWEYFFDHLGEKDEFLAAGK
                     EIQHPFLESVLQEVGKAILQTEPKPTQTLLIQMTNQPFIHGASWISGRLFNILFFPDD
                     QIGMIAVASPWDMKNVTFARFSCTEVDGEQRLLLEPLTSSSTQ"
BASE COUNT          470 a          486 c          455 g          433 t
ORIGIN      
        1 gtccactcac cgcgccggta tcgcaccagg ccgctcccat tggtgcccat ccagatttgg
       61 ggcgtcccgt ccatatcaat ggtttcgagc aagctggtga tggtgttatt ggcgggcttc
      121 cagtccaatc caaacttggc ccaggaactt ttctgaatcc gcgacacgcc cgcactgtta
      181 aatccaaccc aaatgctgct ctccccattg ataccatcag tcgccagtaa actgatggcc
      241 acctgctgac ggttatcggc aatattacca acctgtcgcc actggttttg aaacagacaa
      301 tggatgccac taaaggtcgc aacccagagc cggggttctc cgcgatgact gcgcgtttcc
      361 cgcagggcaa tcaccgggga atttggccga cttgacgaag gaatcaggac cttttcaagc
      421 ctgccgtttt gaagacaggc taatccagat tccatgccaa tccacagcct gggctgaccg
      481 gcagtctcgc tggtttcaac caaacaatgc acggtggtat caggcaattt cccggcaact
      541 ggttcaaagc gatgccacgc cccctcttcc agataggcca gtcccccgcc aagcgacccg
      601 acccataatc ttcgggttgt gccagtctgg gtttccaaaa aacatcgaat gttgttggtt
      661 ggcaaccagg ggcgcatatc aacctgggtc cactggccct ggtgatagcg cacaatgcca
      721 ttcccaacag ttcccgccca aatggtcgaa gttccatctg gttcctgggt ttcatacagg
      781 gtgcgaataa agttgcctgg caggtgcccg gtctgggttg aaaaaagctc ccattcgcca
      841 tgcttaaaat gggcaattcc accgccgttt aacccaaccc acagactttt gtcgctggct
      901 gggagcaagg catagacaaa cgtggaaaga atatttttcg gaaattgaac ccgctgccac
      961 tgtcggccat tgtaacaggc aatcccatcc tgagttccaa cccagacata ctgattccat
     1021 tcgcaaatgg cttcgactga attttgaggg agaccgtttt gatcggtaaa ctgttgatag
     1081 acccaatctt cgcgggcatt attcaggaca gcgttgtccg ttttctccgc caccgctgga
     1141 cggggggaga ttcgttgagc agtgaccatt cctggcagac agatcagtcc ccaaatcccg
     1201 agccaaagac agatgatcac gtggtgggca aacggtcgct gtggcatgaa tggtggtcaa
     1261 aaccaggaac tgagcggtaa ggtggctact gagtggagga actcgtcaaa ggttcaagca
     1321 ggagccgttg ttcgccatca acttcggtgc atgaaaaacg ggcaaacgtg acatttttca
     1381 tatcccaggg ggaggcgacg gcgatcattc caatctggtc atctggaaaa aacagaatgt
     1441 tgaacaaccg tcccgagatc caactggcac catgaataaa tggctgattc gtcatctgga
     1501 tcaacagcgt ttgcgtcggt tttggttcgg tttggagaat cgcttttccg acttcttgca
     1561 aaaccgattc taaaaaagga tgctgaattt ccttcccggc agccagaaat tcgtcttttt
     1621 cacccagatg atcaaaaaag tattcccacg gttccttcag gttttgcgcg gtttggactt
     1681 ttcgcttcag ctcagccagt ggttgatcaa agttcgcagc catagtaccc ctttgaatga
     1741 agaatgaaga ataaagaatt gagaatgaag aatgaagaat tgagaatgaa gaatgaagaa
     1801 tgaagaattg agaatgacct caattttgcc caacatcgca ccac
//
LOCUS       DAOQDO010001437         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65420_length_1844_cov_3.674120, whole genome shotgun sequence.
ACCESSION   DAOQDO010001437 DAOQDO010000000
VERSION     DAOQDO010001437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65420_length_1844_cov_3.674120"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..916
                     /locus_tag="PKZ53_19530"
     CDS             74..916
                     /locus_tag="PKZ53_19530"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ42688.1"
                     /translation="MKFITAIRLAVMMVLAAIFFSIPLVIALPLSPKWRRKVMFYPWF
                     GFANSLRLLFGATVQVTGREYVGGEYEQHHLYICNHLSMADIPILVTVYPLPFVAKKE
                     VMKVPIIGWAGLMSGNVSFDRSDPVARKKVVYETLRRIKEVNSIYIFPEGTRSKTGIP
                     KAEPYLSMMAAAWTEKIDVIPMAIHGSKNVLTDLTSDVRIKLDPPMRCADYPNADRFA
                     QACWSRVIEMFYELNADAKAAGSRTAIPAEMVPPTPATASTRLNEVFESVSQEIHDEP
                     STTE"
     gene            891..>1844
                     /gene="era"
                     /locus_tag="PKZ53_19535"
     CDS             891..>1844
                     /gene="era"
                     /locus_tag="PKZ53_19535"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNJ42689.1"
                     /translation="MTNPPRPNDSPEVEPTDTAQAVELEVEADQTTEIEVETDDAGTD
                     EVEALEVETDDADDLEDEDDEDEDELSDVEMDEVAPTPASFRSGFVGILGRPNAGKST
                     LINHLVGEKIAAVSDKPQTTRTRIQGIVTLPDEGQIVFVDTPGVHKPGYKLNKRMMQA
                     VLDTIQTVDLLLLLRDASVSTGNGDKFVLDMVKQAGKPTFLLLNKIDKVADKTALLKL
                     MEFYRTEYEFVEFIPLSGLKGDNTKLLVQKILEHLPVGPPLFEEDALTDQTDRMLVAE
                     LIREQALRALSQELPFSIAVMTEQWETRPNGVVHLACVIYVE"
BASE COUNT          469 a          493 c          460 g          422 t
ORIGIN      
        1 gcgacattac gccggagttt gactaaagac aaagcggcgt caagccgccg cactccatac
       61 agggggtacc caggtgaaat ttattactgc catccgactc gcggtgatga tggtgctggc
      121 ggccattttc ttttcgattc cgctggtaat tgccctgcct ctttcaccca aatggcggcg
      181 caaagtcatg ttttacccgt ggtttggatt tgccaattca ttacgactgc tttttggagc
      241 aacggttcag gttaccggtc gcgaatatgt cgggggcgag tatgagcaac atcacctcta
      301 tatttgcaat cacctgagca tggccgatat cccgattctg gtcacggtgt atccactccc
      361 gtttgtggcc aaaaaagagg tcatgaaggt tccaatcatc ggctgggcag gtttgatgtc
      421 tggaaatgtc tcgtttgatc gctcggatcc cgttgcccgt aaaaaagtcg tctatgaaac
      481 cctccggcga atcaaggaag tcaactcgat ttatattttt ccagagggca cgcgcagcaa
      541 aaccgggatt cccaaagccg aaccgtacct ctcgatgatg gccgctgcct ggaccgaaaa
      601 aattgatgtg atcccgatgg ccattcacgg ctctaaaaat gtgctcactg acttaaccag
      661 tgatgtccgt atcaagctgg atccaccgat gcggtgcgca gactatccca acgctgaccg
      721 gtttgcccag gcctgttgga gccgggtcat cgagatgttt tatgaactca acgccgatgc
      781 caaagcggct ggcagtcgaa ccgcaattcc agccgaaatg gtaccaccca caccagcaac
      841 ggcctcaacc cgactcaatg aagtctttga gtcagtttcc caggaaatcc atgacgaacc
      901 ctccacgacc gaatgactcg cccgaagttg aacccactga cacagcgcaa gcggttgaac
      961 tcgaagtcga agctgaccaa accacggaaa ttgaagttga gaccgatgac gccgggactg
     1021 acgaggtcga ggcacttgaa gttgagaccg atgacgctga tgatcttgaa gatgaagatg
     1081 atgaagatga agatgaattg tcagacgttg aaatggatga ggttgccccg actccggcct
     1141 catttcgatc tggctttgtt ggcattctgg gccgccccaa cgctggcaaa tcaacgctga
     1201 tcaatcatct ggtcggtgaa aaaatcgccg ctgtttccga taaaccccaa accacccgca
     1261 cccgcatcca ggggattgtg accttgcctg acgaaggcca aattgtgttc gtagacacac
     1321 ccggcgttca taaaccagga tacaaactca acaagcgaat gatgcaggca gtcctggata
     1381 ccattcaaac agttgatctt ttgctcttgc tacgtgatgc ttcggtttca accggaaacg
     1441 gtgataaatt tgtcctggac atggtcaaac aggccgggaa accaacattt ttgctgctga
     1501 acaaaattga taaagtcgcc gacaaaaccg cactcttgaa gttgatggag ttttaccgaa
     1561 ccgaatatga gtttgtggaa ttcatcccac tttcgggtct caaaggcgac aacaccaagc
     1621 tcctggttca gaaaatcctc gaacacctgc ccgttggccc gcctctgttt gaagaagacg
     1681 ccctgaccga ccagaccgac cgcatgctgg tggcggaact gattcgcgag caggcattgc
     1741 gggcgctctc acaagaactt ccgttctcaa ttgccgtgat gaccgaacaa tgggaaaccc
     1801 gcccaaatgg cgtggttcat ctggcctgtg tcatctatgt cgaa
//
LOCUS       DAOQDO010001438         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65490_length_1843_cov_3.384787, whole genome shotgun sequence.
ACCESSION   DAOQDO010001438 DAOQDO010000000
VERSION     DAOQDO010001438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65490_length_1843_cov_3.384787"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKZ53_19540"
     CDS             complement(<1..741)
                     /locus_tag="PKZ53_19540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ42690.1"
                     /translation="MFPKIVMRSYVLAVVYSCVILCLLSGMKGISRVYGQSGSTTGMI
                     VGRVKDVGGAVIAGAPVKARQIETNLERTADADEDGNYQFLQLPPGNYEVMVELEGFA
                     IKKERVLINIGTSALVQLTLVPAGATEIVKVEAAVSFEQGKTESSAINGSDRIDNLPI
                     NRRNFLDFAVTAPRVTVDRLPVQGVAATSGLSFNGQSSRQNNVTIDGLDNNDSNVGTV
                     RSTFSQEAVREFQVVSDSYSAEFGRALGG"
     gene            complement(1055..>1843)
                     /locus_tag="PKZ53_19545"
     CDS             complement(1055..>1843)
                     /locus_tag="PKZ53_19545"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HNJ42691.1"
                     /translation="IGGSVFCYMELASALGQERPFFGFQAMGLEPGQRPFQTVEQMAA
                     CYIASLRVVQPKGPYLLGGWSFGGLIAFEMAHQLRAQDQSQCTLILLDSPPPHSPDWP
                     SSTDARQILSWMQTDLFGLSKTADQPAGIKIETSANSDSIDQIESTTLEAQMESLWEQ
                     AVNAGVFAVDSTRSKLDQLFALYQAHIQAGSTYAPSPLAGRIILIEAVTQPQHFHLSL
                     LSGWQDLSETPIEYQQLEADHFSLMTSPQVQYLARLVTQAITSA"
BASE COUNT          458 a          462 c          477 g          446 t
ORIGIN      
        1 accacccaga gcgcggccaa attcagccga atagctgtct gaaaccacct gaaattcgcg
       61 cacggcttcc tgactaaatg ttgaccggac ggtgccaacg ttgctgtcat tgttatccaa
      121 tccatcaatg gtgacgttgt tttggcggct tgactgacca ttaaacgata atcctgaggt
      181 ggcggctacc ccttgaacgg gtaagcggtc aactgttact cgtggcgcag taacggcaaa
      241 atccaggaag ttccggcggt tgatgggcag attgtcaatt cggtctgacc cattgatggc
      301 gctgctttca gtcttgcctt gctcaaaaga aaccgctgcc tcaaccttga cgatttcggt
      361 ggcgcctgca ggcaccaggg tcagttgaac cagggctgat gtcccgatat tgatcaggac
      421 tcgttctttt ttgatcgcaa aaccttcgag ttccaccatc acttcataat tgccaggcgg
      481 caattgcaaa aattgatagt tgccgtcctc atcagcgtca gcggtccgtt caagattggt
      541 ctcaatttgt ctggctttga ccggggcacc ggcaatgacg gcccccccca catctttgac
      601 ccgaccaaca atcatgccgg tagttgagcc gctctggcca tatacccgtg aaattccctt
      661 cattccagat aaaaggcata aaatgacaca gctatagacc acagccaaca catagcttcg
      721 catgacgatc ttcggaaaca tagttcacct tcgagaaaag gaattcaaag aagaaagacc
      781 cacgttggat ttcaacagag acgaaaccat cctggatctt gtgcagattg ggaaagaccc
      841 aattgcctga ctggatggca gttggtgcga acgtgaaaac ctggatggac ttgcaagaga
      901 cagcaactga tatggagaag aaaaggactt gctgttcaat tttttgacct ggagtgaccc
      961 tggttggatg gatagcggct ggcatcatag cattgtttcc agtgacattc caaatttctc
     1021 caacattctg tgagatgact tcatcgcaaa aacttcaggc ggaagtgatt gcctgtgtga
     1081 ccaaccgggc cagatattga acctgaggcg aggtcatcaa cgagaaatga tcggcttcca
     1141 gttgttgata ttcaattggg gtttcactca agtcttgcca gcccgaaagg agtgataaat
     1201 ggaaatgttg gggctgcgta acggcttcaa tgagaattat tcgaccggcc agcggtgacg
     1261 gggcgtaggt tgacccggcc tggatatgtg cctggtagag agcaaagagt tgatccagct
     1321 tgcttcgcgt tgaatcaacg gcaaacacgc cggcgttgac cgcctgttcc cagagggact
     1381 ccatctgtgc ctccagggtg gttgattcga tctgatcaat tgaatctgaa ttcgctgaag
     1441 tttcgatttt gattcctgcc ggctggtcgg ctgtcttcga taacccaaat aaatccgttt
     1501 gcatccagct caggatctgc cgggcatcgg ttgaagatgg ccagtcgggt gaatgaggtg
     1561 gtgggctgtc cagcagtatc agggtgcatt gagattgatc ctgagccctg agctgatggg
     1621 ccatttcaaa ggctatcagc ccgccaaacg accagcctcc aagaagatag ggacctttgg
     1681 gttgtaccac tcgcaggctg gcgatatagc aggctgccat ttgttcgacg gtctggaatg
     1741 gcctttgtcc tggctccaga cccattgcct ggaatccaaa aaagggacgc tcttgcccca
     1801 gggctgaagc cagctccata taacaaaaca cactccctcc aat
//
LOCUS       DAOQDO010001439         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65505_length_1843_cov_2.840045, whole genome shotgun sequence.
ACCESSION   DAOQDO010001439 DAOQDO010000000
VERSION     DAOQDO010001439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65505_length_1843_cov_2.840045"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..>1843
                     /locus_tag="PKZ53_19550"
     CDS             243..>1843
                     /locus_tag="PKZ53_19550"
                     /inference="COORDINATES: protein motif:HMM:NF014894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ42692.1"
                     /translation="MVKKIDPEILMGFIEEAKSYLPTILDCIETFKHDPDQLDALEEA
                     HRLFHSIKGAASMVGLSALSHVAYFAEEALEEIAAGQLEFTPEATTVFLLTVSQIESY
                     LDGMLSGTLDDHAILVEVVKSFRRLKHLSPSGDEAAIETLVTHSQPSVEPSQPVVAVK
                     PPVEEHKTLEVKPVIISTARRTDHIDEASLELREAFLEEADEHLQNITRVLRLLEKDP
                     TNKGWQQEIRRSVHTLKGAAGMVGFQTVSRLAHRMEDLLDAVYEDDLELTPEIIALIF
                     VTTDTLEDLLHKGSAEVRERAEDLSQRFAAFLGTELANLEPVSGGNRLEPLGQESMID
                     LAGMLPQRRAEDQAAEEEHPESQRQPSQVVRVPIERLDELVRLVSELIVSRSTFEQYF
                     RKLNHEVDELRLSVGRSQRIANRFETEYEVSALGKGMASLVNVAAVGAGPGVTLSASP
                     VKQTHGFDELEFDRYTEFHLLTRELSENTGDINAVSSELHNTIGDFDSYLNGLNRLTS
                     EVQDKLMRLRMVPVGSLTARLHRTVR"
BASE COUNT          477 a          484 c          486 g          396 t
ORIGIN      
        1 ggcaagcacc agactgcaag atcattgagt gaactcgaat ttcaacaaaa tcgagccttc
       61 acggtgacaa aaccgacttc cacccggtcc tttctgtcct ttttgtcctt tgattttggc
      121 ccaatcaacc atctgagaaa ccacaactga ggaaaagatc ggtgttttgg gcgaaatttg
      181 gggaatgaag aatcatccta atctgaaacc ggtattcggg gcgaaccgag gaaagagtcc
      241 ttatggtgaa aaaaattgat ccagaaattc tgatgggatt tatcgaagaa gccaaaagct
      301 atcttcccac catccttgat tgcattgaaa cgtttaagca cgatcctgac caactcgatg
      361 cgctcgaaga agcccaccgg ctgtttcata gtatcaaagg ggcggcttcc atggttggac
      421 tctcggccct gagccatgtt gcctactttg cggaagaagc cctggaagaa atcgccgccg
      481 gtcaacttga gtttacgcca gaggccacca ctgttttcct gttgacggtc agccagatcg
      541 agtcttatct ggacggtatg ctctctggga cactcgatga tcatgccatc ctggtggaag
      601 tggtcaaaag ctttcggcga ctcaaacacc tttccccttc gggtgatgaa gccgccattg
      661 aaacactggt cacccacagc cagccttcgg tggaaccatc gcaaccagta gttgcagtca
      721 aaccgccagt tgaagaacac aaaacccttg aagtcaaacc agtcatcatc agtaccgctc
      781 gccggacaga tcatattgac gaagcgtcac ttgaactgcg cgaagccttt ttggaagaag
      841 ccgacgaaca cctccaaaat atcacccgtg tcctgcggtt gcttgaaaaa gatccgacga
      901 acaaaggctg gcaacaggaa atccgccgga gcgtgcatac gctgaaaggg gccgcgggaa
      961 tggtcgggtt tcaaaccgtc agccggctgg cgcatcggat ggaagatttg ttggatgcgg
     1021 tctatgaaga cgacctggaa ctgacgccag aaattatcgc gctgatcttt gtcaccactg
     1081 ataccctcga agacctgctc cataaaggca gtgctgaagt ccgtgagcgc gccgaagatc
     1141 tctctcaacg ctttgcggca tttctgggga cggaactcgc aaacctggag ccagtttcag
     1201 gtggaaatcg ccttgaaccc ctgggccagg aaagcatgat tgacctggct gggatgcttc
     1261 cccagcggag agccgaagac caggcggcgg aagaagagca ccccgagagc cagcggcagc
     1321 catcccaggt ggtgcgagta ccgattgaac ggctggatga actggttcgg ctggtcagtg
     1381 aattgatcgt cagccgctcc acctttgaac agtatttcag aaaactcaac cacgaagttg
     1441 acgaactccg attgagtgtc gggcggtcac aacgcattgc caaccggttt gaaaccgaat
     1501 atgaggtgag tgccctgggt aaaggaatgg catcgctggt caacgtggct gcagttggcg
     1561 ctggacctgg ggttacgctt tcagcgtctc cggtaaagca aacccacggc tttgacgaac
     1621 tggaatttga ccgctacacc gaatttcacc tcctgacccg tgaactcagt gaaaatacag
     1681 gcgatatcaa cgccgttagc agtgaactcc ataacaccat cggcgatttt gacagctatc
     1741 tcaacggctt aaatcggctg acgagcgaag tccaggacaa attgatgcgg ttacggatgg
     1801 tgccagtcgg gtcgttgacg gcgcggctcc atcgcacagt tcg
//
LOCUS       DAOQDO010001440         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65632_length_1841_cov_4.702128, whole genome shotgun sequence.
ACCESSION   DAOQDO010001440 DAOQDO010000000
VERSION     DAOQDO010001440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65632_length_1841_cov_4.702128"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKZ53_19555"
     CDS             complement(<1..637)
                     /locus_tag="PKZ53_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ42693.1"
                     /translation="MKFAEHIAVIGAGSFGTALALSIARYTPTGRPVRDVRLWSHRGE
                     HAEQMTQERLNATYLPGFTFPDNLTPTSDLAQALDGANIVLTVVPSHVTRETYEKMRP
                     FLKPQMVFVNGTKGIEGDTLKRMSQVISDVLSEDFEPRMVTLSGPSFAQEVAKGDPTA
                     VVAASTTQRWSEYVQAELSNPNFRLYSNLDMVGVEIGAAVKNVIAIAAGAVT"
     gene            complement(746..1390)
                     /gene="plsY"
                     /locus_tag="PKZ53_19560"
     CDS             complement(746..1390)
                     /gene="plsY"
                     /locus_tag="PKZ53_19560"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNJ42694.1"
                     /translation="MSLVFWSGILFLAYLLGSFPSGYLIVRLLTQDDVRHIGSGSTGA
                     TNVTRKAGVKAGLLTYVLDVGKGMLAVYVAGQLSGHNVHVMGSAGAIAILGHMFPIFL
                     KFQGGKGVATGVGVFLLLAPVPTLAVFAVWGLIFALTRTVSLGSVVATGLLPVAIWAF
                     EGLLAGKPASVWIPKLLWGLGIGLLIISKHHENIRRLLSGTESSFKKQNSEVVK"
BASE COUNT          472 a          473 c          493 g          403 t
ORIGIN      
        1 ccgtcacggc gccagcagca atcgcgatta cgtttttgac cgcggcgccg atttccacac
       61 cgaccatatc gaggttggag tacaaccgga agttagggtt gctgagttca gcctggacat
      121 attcgctcca acgttgggtg gtcgaagccg cgaccacggc ggtcgggtca cctttggcga
      181 cttcctgggc aaagctgggg ccagacaggg tcaccatacg cggttcaaaa tcttcactca
      241 acacatcgga aatcacctgt gacatccgct tcaaggtatc accctcaatg cctttggtgc
      301 cgttgacaaa caccatttgc ggtttcagaa atggccgcat tttttcatag gtttcgcggg
      361 tgacgtgtga gggaacaacc gtcaacacaa tgtttgcccc atcgagtgcc tgtgccagat
      421 ccgaggttgg tgtcaggttg tcaggaaagg taaatccagg gagataggtg gcattcagtc
      481 gctcctgggt catttgctcg gcatgttcac cccgatgaga ccagagtcga acgtcccgca
      541 cgggtcttcc tgtcggggta tatcgagcaa tactgagtgc cagggcggtt ccaaaactgc
      601 cagcgccgat gacggcgatg tgttctgcaa atttcatatg agcgaagccg tgaaagagtg
      661 aagtggcgaa gtcgtgaagc ggtgaaataa ctcagcgttc tgtttcagat tagtttctgg
      721 caactcctaa acgctttgtc tttggctatt ttactacttc actgttttgt tttttgaatg
      781 agctttcggt gccggaaagc aatcgtcgaa tattttcgtg gtgtttggag ataatcagta
      841 agccaatccc caatccccac aagagtttag gtatccaaac cgaagccggt ttcccagcca
      901 gaagaccttc aaaagcccaa attgcaactg gcagcaaacc agttgccaca actgagccga
      961 gcgagacggt tcgggtcagg gcaaaaatca gtccccacac agcaaacacg gccagggttg
     1021 ggactggtgc caggagcaga aagacgccaa ccccagtggc cacgcctttg ccaccctgga
     1081 attttaagaa aatcggaaac atgtggccga gaatggctat ggcgccggca cttcccatga
     1141 catggacatt gtgtccgctg agttgaccgg ccacatagac ggcaagcatt ccttttccga
     1201 catccagcac ataggtcagt aagccagcct tgaccccggc tttgcgggtg acattggtgg
     1261 cacctgtgct gcctgagcca atgtggcgaa catcatcctg ggtcaacaag cggacgatca
     1321 gataccccga agggaatgat cccaaaaggt atgcgagaaa aagaatgcca gaccagaaaa
     1381 caagcgacat agggttttgt gagaggcaca ggtatgaaat gaagtgatgt tgaccaatca
     1441 agattttcga agaacccaga acccggaatg tgaggtcaat acttgcctgt aagcgcaatg
     1501 gggcgcaata taacaaccac cttcggtgag cacaagttct tacgaattat gccttgtcaa
     1561 gagactcggc cctcaagggc caagcttgag tcgtgtaccc acgacgctat cctaacagat
     1621 agccgacatt ggtagactca ctcaagtttc acttgaccag acccccgtgc aaatcacggg
     1681 aaacgattgg gaagaatcca taggtaccag cggatgccgc ctcagtcggc tgctctacgg
     1741 tgacaggtta aacagggatg ggagtcagtg ccggtgcctg tcatagtcaa accttcccaa
     1801 atccggtcga gaggaagtcg aatccggtcg gcggtcacag t
//
LOCUS       DAOQDO010001441         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65710_length_1840_cov_3.838095, whole genome shotgun sequence.
ACCESSION   DAOQDO010001441 DAOQDO010000000
VERSION     DAOQDO010001441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65710_length_1840_cov_3.838095"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKZ53_19565"
     CDS             <1..493
                     /locus_tag="PKZ53_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ42695.1"
                     /translation="VYVAPERLNSDAYDGKSDVYSVGIMLFQMLTGALPFNISGNNPM
                     QVFLATLHKTPPELHTINPNLQPEISAVVLSALEKSPHQRPGAWELACAFVEAVNQSG
                     ISESSAWIPPSLTQEAERPRFETDPGWNVQTLGNQPLDNEPTQIAEPQARKLGAEDSQ
                     ARG"
     gene            801..>1840
                     /locus_tag="PKZ53_19570"
     CDS             801..>1840
                     /locus_tag="PKZ53_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42696.1"
                     /translation="MVWTAVARHFGWVWLIGLIGTGLSGMVWAQSPAQPAQAGMRFVH
                     NYTVEEYKAADQNWVVIQDLRGVMYFANNNGVLEYDGATWRSILLPNRSPVRALAMDA
                     QGRVFVGGANELGYLAPDEQGQQQFVSLLEFIPEFAHRFADVLRMYATPDGVFFQTSH
                     FFFIWDGQRMTVIRPALAGDTFSDCQEINHEIFLVEPSRGLVKLFHRTLQPVPGGQWL
                     KEKRLSGLLPMPDGNRLAVVRGEGLLLYDGQSFTPFAPDLSKALALWDPLHTLVLPDG
                     RFAIATRIHGVVILRPDGTLDEVIGKTAGLQSKLVHFLSLDRSGNLWMALDKGLARAE
                     LISPFTCFDERLG"
BASE COUNT          386 a          521 c          494 g          439 t
ORIGIN      
        1 ggtttatgtc gctccggaac gactcaatag cgacgcctat gacggcaaat cagacgtcta
       61 cagtgtcgga atcatgctct ttcaaatgtt gaccggcgcg ttgccattca atatctctgg
      121 caacaacccg atgcaggtgt ttctcgccac actccacaaa acacctccag agctgcacac
      181 catcaacccg aaccttcagc ctgaaatcag cgccgtcgta ttgtctgccc tggagaaaag
      241 cccgcaccag cgtccaggtg cgtgggaact ggcctgtgct tttgtcgaag ccgtcaatca
      301 gtcaggcatt tcagaaagct cagcgtggat tcctccgagc ctgacacaag aagccgaacg
      361 cccacgcttt gaaaccgatc ccggatggaa tgttcaaaca cttggcaacc aaccacttga
      421 caacgagcca acccagattg ccgagccgca ggctcgcaag ctcggggctg aagactcgca
      481 ggctcggggc tgaaagcttg gggctgaaga ctcgcaggct cggggcggaa gactcgcaag
      541 ctcagggctg aaagcttggg gctgaagaag atggggtcaa tctcctgagc ccatcttctt
      601 gagcctgatg tcttccgccc gatgtcttcc gccccaagcc cgatgtcttc cgcccacctt
      661 cttcagcccc aagccccaag cccgatgtct tccgcccgat gtcttccacc cgatgccttc
      721 cgccctgaac agtgctacac tgcggccttt ccgttattcc gcaggcaaca cacaaatttt
      781 tcggtaccgg attttcccct atggtctgga cggcagtagc acgtcatttt ggctgggtgt
      841 ggttgattgg tctcatcgga actggtctat ctggaatggt ttgggcgcag tcaccggctc
      901 aaccggcaca ggccggaatg cggtttgtac acaattacac agttgaagaa tataaagcgg
      961 ctgatcaaaa ctgggtggtc atccaggatt tgcgcggggt gatgtatttc gccaataaca
     1021 atggtgtcct ggaatatgac ggcgccacct ggcgatcaat cttattgccc aaccggtctc
     1081 ccgtccgggc actggcgatg gatgcccagg gccgcgtttt tgtcggaggc gcgaatgaac
     1141 ttggctacct ggcgcctgat gaacaggggc agcagcaatt tgtttcatta ctagagttta
     1201 tcccagagtt tgcccaccgt ttcgcggatg tcttgcgcat gtatgccaca cccgatggcg
     1261 ttttttttca gaccagtcat ttctttttca tttgggatgg tcagcggatg acggtgattc
     1321 gcccggcact ggcaggtgat accttttctg attgtcagga gatcaatcac gaaatttttc
     1381 tggtggaacc atcgcgcggg ctggtcaagc tctttcatcg aaccttgcag cctgtccccg
     1441 gcggtcagtg gttgaaagaa aaacggttat cgggcttgct cccgatgccg gatggcaacc
     1501 ggctggccgt ggttcgcggt gaaggactcc tgctctatga cggacaaagt tttaccccct
     1561 ttgccccgga tctttccaaa gcattggccc tttgggatcc actccacacg cttgttttgc
     1621 cggatggacg ctttgcgatt gcgacgcgca ttcacggcgt agtgattttg cgtccagatg
     1681 gcacacttga cgaagtcatc ggcaaaaccg ccggactcca aagcaagctg gttcattttc
     1741 tcagcctgga ccggagcggc aacctctgga tggcactgga taaaggactg gcccgggcag
     1801 agttgatctc accttttacc tgttttgatg agcggttggg
//
LOCUS       DAOQDO010001442         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65713_length_1840_cov_3.378711, whole genome shotgun sequence.
ACCESSION   DAOQDO010001442 DAOQDO010000000
VERSION     DAOQDO010001442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65713_length_1840_cov_3.378711"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKZ53_19575"
     CDS             complement(<1..189)
                     /locus_tag="PKZ53_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42697.1"
                     /translation="MHRKIFVRGQFQTWFRLFMLGLVVLASSQLTPAQTKPALAVTAE
                     QGRENIRKAVNYEALIKNS"
     gene            complement(464..>1840)
                     /locus_tag="PKZ53_19580"
     CDS             complement(464..>1840)
                     /locus_tag="PKZ53_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNJ42698.1"
                     /translation="LLWVPFLVAMFPHSVFSQTPARDFSKETSRPKPAWIQDAVIYEI
                     FPRTFSAKGNFAGVTEQLDRLKTLGVDVIWLMPIHPIGEVKRKGTYGSPYAVKDYYAI
                     NPDYGTAEDLKKLVREAHQRGLKVIIDIVANHTGWDSVMMKNPEFYVQNAAGQIISPY
                     PDWTDVADLNYDNPKLRDYMTSMLKYWLQEFDLDGFRCDVAGFVPTSFWDQARVELEK
                     VKPNLIMLAEWDQPDLLVKAFDIDYAWPFHKALDGVLTGKEPATLLRKVWETERNTFP
                     AGSLHLRFSDNHDERRAIVRFGEHGALAAQALMFTLDGVPLIYNGMEVGDTAESGAPA
                     LFEKVPILWQIGERRPEFARFYSQMVPLRKKHAALRQGETIWLQNSDESHIVSYLRRS
                     GGEEFLVVINFSNRPFRGFVEVNRGNQFEEVTPDLTDGKTQRETIVSLPTITLDPMGF
                     RIFRRATK"
BASE COUNT          440 a          434 c          467 g          499 t
ORIGIN      
        1 tgaatttttg atcaaagcct cataattgac tgctttgcgg atgttttcgc gaccctgctc
       61 agccgttact gccagagcgg gtttggtttg agccggcgtg agctggctac tggccaaaac
      121 caccagtcca agcatgaaca gccggaacca ggtttggaat tgaccgcgaa caaatatttt
      181 tcggtgcatg tggtccatcc ttataccagt cagtagtcag tagtcagtag tcagtagttc
      241 gctaagttta tttggttgaa ttactgaact gtctgctaaa gggttgttgt tgggtagttg
      301 tggtgcgaag cccacggtct cggaagccaa tgttcaaggt tgaacaattc gctatttgtt
      361 tatttttcaa cgttttacag ccagaggttt gttgtctgga tgaagccgat tcatttgttc
      421 ctggtggttt caccactcgc tcagcccgtg tggtttttcc tggctacttc gttgctctcc
      481 gaaaaatgcg gaaacccatc ggatcgaggg tgatcgtggg aagcgagaca attgtttcac
      541 gttgtgtttt gccatcggtc aaatctggag tgacttcttc aaactgattc cctcggttga
      601 cttcgacaaa tccacgaaat ggacgattgg aaaaattgat tacaaccagg aattcttcac
      661 caccggaccg ccgcaaatac gacacgatat gtgattcatc agaattttgc agccagatgg
      721 tttcgccctg tcggagggcg gcatgttttt ttctcagtgg aaccatctgg ctgtaaaacc
      781 gggcaaattc ggggcggcgt tcaccgattt gccataaaat cggaactttt tcaaacagag
      841 caggtgcccc cgattcggca gtgtctccaa cttccatgcc gttgtaaatc aagggtacac
      901 cgtcaagggt aaacatcagt gcctgggcag ccagcgcccc gtgttcgcca aagcggacaa
      961 tggcccgtcg ttcgtcatgg ttgtctgaaa atcgaagatg gagcgaaccg gctggaaagg
     1021 tgttgcgctc ggtttcccat acttttcgga gcaatgtcgc cggttccttg ccggttaaca
     1081 caccatcgag cgctttgtga aagggccagg catagtcaat gtcaaaagcc ttgaccagca
     1141 ggtctggttg atcccattct gccagcataa tcagattggg tttgactttt tcgagttcaa
     1201 cccgtgcctg atcccagaaa ctggtcggta caaaaccggc aacatcgcat cgaaatccat
     1261 ccaaatcaaa ctcttgcagc cagtatttga gcatgctggt catatagtcc cgaagcttcg
     1321 gattgtcata attgagatcg gccacatctg tccagtccgg gtaaggtgaa ataatctgcc
     1381 cggcggcatt ctggacgtaa aattcgggat ttttcatcat cacactatcc cagccggtat
     1441 gattggccac aatgtcaata atgactttta acccgcgttg atgggcttca cgaaccagtt
     1501 ttttaaggtc ttctgcggtg ccatagtcag ggttaatcgc ataatagtct ttgacggcat
     1561 atgggctgcc atacgtccct ttgcgtttga cttcgccaat tgggtggatt ggcatcaacc
     1621 agatcacgtc cacgccgagt gttttgagtc ggtcaagttg ttcggttacg cccgcaaaat
     1681 tgcctttggc ggaaaacgtg cgcgggaaaa tttcataaat gactgcgtcc tgaatccagg
     1741 caggtttggg acgcgaggtt tcttttgaga aatcacgggc aggtgtttgc gaaaaaacag
     1801 aatgtgggaa catagccacc agaaacggta cccagagcaa
//
LOCUS       DAOQDO010001443         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65755_length_1840_cov_1.767507, whole genome shotgun sequence.
ACCESSION   DAOQDO010001443 DAOQDO010000000
VERSION     DAOQDO010001443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65755_length_1840_cov_1.767507"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..987
                     /locus_tag="PKZ53_19585"
     CDS             271..987
                     /locus_tag="PKZ53_19585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ42699.1"
                     /translation="MRSRNNPWPAFVDLFSALLITTLGGFILLSGVYDQDVKIYQQKE
                     EQLKQIRQEAKQSIDQVQTSLGSKTVVKEVRATETERFFDLDIRFGTSDDTLDQEGSR
                     RIAEICHEIRSIFNNLSSDQKRDIEIIIEGHTDSQQAKIVDQREKDRFNWNLSARRAT
                     SVLYEFAQNGIRSPEYQIVATGFADSRPLCQDLTPECYSRNRRTTLCLRVNTKNIEDR
                     LESLLKGEGNPNQVNLPEKK"
     gene            complement(1024..>1840)
                     /locus_tag="PKZ53_19590"
     CDS             complement(1024..>1840)
                     /locus_tag="PKZ53_19590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ42700.1"
                     /translation="QNEQEVTRSLQDLNRMKTNFIVVTSHEMRTPLTVLKGFHEVLLS
                     EYLGLLTGAQRQSLETCQRMVDRLNANFDDILEMLKINEGATALNVTEFDLRTVINEI
                     HTEVSVFLDRRHQKLMIDCPEVLDIKADRAKLQLILMEIIQNAIKFSYDGGIIFLNVA
                     IDNHQLQVTVKDQGIGIDKSELSKIFDQFYTSADPSTHSSGRYEFGARGAGLGLSIAR
                     SYTESHGGRIWAESDGPGQGSRIQVRIPLHTVVSGQPQPQIPSATTFGSVLEK"
BASE COUNT          513 a          423 c          428 g          476 t
ORIGIN      
        1 ccaagctcat ccacggttca atccgaatca gttccccttg tagtacaaag tgaagaatat
       61 gcagtccaac ccccagggat tgaacatgat attcaaatat tgacgccttt tggtgatgga
      121 cgtgacccct tttcaacctt tcccgtctca cccccagcac caaatattat ccaaccaact
      181 gaactgaatc agccccaagg aacacaccca ctctacacac ctcgcaaacc agaattggaa
      241 tagtcggttg gatattcagg gagaaaacct atgaggagtc gaaacaaccc atggccagct
      301 tttgtagatt tattctctgc actgttaatt actactttgg gtgggtttat cttgctatca
      361 ggtgtttatg accaggatgt gaagatctat cagcaaaaag aagaacaact aaagcaaatt
      421 cgccaggaag cgaaacaatc cattgatcaa gtccaaactt cattagggtc aaaaactgtt
      481 gtcaaagaag ttcgagcaac tgagacggaa aggttttttg atcttgatat caggtttggg
      541 acaagcgatg acacgttgga tcaagaaggc tcaagaagaa tcgctgaaat ttgccacgag
      601 atccgttcaa tcttcaacaa tctctctagt gatcaaaaac gggatattga aataattatt
      661 gaaggtcaca cagatagcca acaggctaag attgtagatc aacgtgaaaa agataggttt
      721 aattggaact taagtgcacg acgagcaacc tcagtgcttt atgaatttgc ccaaaatggc
      781 atcagatcac ctgagtacca aattgtggca actgggtttg cagattcccg accactttgc
      841 caggacttaa ctcctgaatg ttattcaaga aatcgtcgaa ccacattgtg ccttcgtgtg
      901 aatacaaaaa atattgaaga tcgccttgaa tctcttctaa aaggggaggg caatcctaat
      961 caagttaact taccagaaaa gaagtgagtc gttgattcta ggaggcaatt tgtatgtttc
     1021 aatttatttc tccaagacgg acccaaacgt tgtagctgat ggaatttgag gctggggctg
     1081 accagaaaca acagtgtgga gcggaatccg gacttgaatc cggctgccct ggccggggcc
     1141 gtcggattcg gcccagatgc ggcctccgtg ggattcggtg taactgcggg cgattgacaa
     1201 tccgagaccg gcgccgcgcg ctccgaactc gtaccgtccg gaggaatgcg tggatggatc
     1261 ggcgctcgta taaaactggt cgaatatttt ggaaagttcg gatttatcta tcccaatccc
     1321 ctggtctttg actgttactt gtagctgatg gttatcaatc gcgacgttca ggaaaataat
     1381 gccgccatca tatgagaatt taatcgcgtt ctgaatgatc tccatcagga tcaactggag
     1441 tttggcccgg tcggctttga tgtcgagcac ttccggacag tcaatcatca gcttttgatg
     1501 ccgacgatca aggaaaaccg aaacttcggt gtggatttcg ttaatgactg tcctcaaatc
     1561 aaactctgtt acattgagcg ccgtggcgcc ttcgttgatt ttgagcattt ccagaatgtc
     1621 atcaaaattg gcgttgaggc ggtccaccat ccgctggcag gtctcaagcg actgacgctg
     1681 ggcacctgtt aacaatccca aatattcgct caagagcact tcgtgaaagc ctttgagcac
     1741 cgtcaacggc gttcgcattt cgtgcgatgt cacgacgata aagttcgtct tcatccggtt
     1801 gagatcctgg aggctgcgcg tgacttcctg ttcgttctgg
//
LOCUS       DAOQDO010001444         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65779_length_1839_cov_3.276345, whole genome shotgun sequence.
ACCESSION   DAOQDO010001444 DAOQDO010000000
VERSION     DAOQDO010001444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65779_length_1839_cov_3.276345"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..1236)
                     /locus_tag="PKZ53_19595"
     CDS             complement(442..1236)
                     /locus_tag="PKZ53_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42701.1"
                     /translation="MSKKFFEAILGLCLLGCLAFGVLSVLSWVFHWSPLPQVSGAVLL
                     NVGMGFLCLTGLMVIMKVPWDLHFEIRALLFEMNLSRERNIAVNADREKYLLRMRWIT
                     LVTAIGAHIGAAAVIAGVTYWSQGQTGYYFAAFYLLATFFRPAHAAHQFLWAKLQEIR
                     REVKYPRDDVISLVNDVRELKVQMEAVNEHLKNIDSQLITGNETDRKLRSDLTELQNV
                     LHQTEQTFHNRMQMLSDEVERSMLKAVDNQDIVSGLRAFAKLIKQA"
     gene            complement(1547..>1839)
                     /locus_tag="PKZ53_19600"
     CDS             complement(1547..>1839)
                     /locus_tag="PKZ53_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42702.1"
                     /translation="HWPEFLRWAHTTLVAAGVDSLALNFRDAREPDWNRGLRACGFLL
                     TDALTAKLIPSDCKAWVFRVLSDTSLAELRTFVEQFLPQKTGAEDVGLKTSG"
BASE COUNT          462 a          463 c          454 g          460 t
ORIGIN      
        1 taatattttc ccgaatcgcc accagatgca gcggtgtatc ttcaagatag agcgacacca
       61 tttcccgaaa aaaactatcg tcatccccat cctgaagatt ggttttcaat gttgtgagga
      121 ctttaaagtc aatggaatca tgctgatcag tcatatttgt caggatcatc aggaaaagca
      181 aaactggaaa tttctcgcgc cgtcaacaca gaggacagtc gaaaagtcct tcaaaatgag
      241 ttgtttccag tttatgtaac ggttagaggg aacttgaata tcaatttgga aagcggcgct
      301 ccaactatta catacccgat tctcaggacg caacatttcc gggttccggg ttccgggttc
      361 cgcgcttccg ggttttcgaa ttttggtcct tggggtcact tatgtccttt atgtcctttg
      421 tgcggaaccc ggaacccaca atcaggcttg cttgatgagt ttggcaaagg cccggagacc
      481 gctcacaatg tcttgattat caaccgcttt gagcattgag cgttcgactt catcactgag
      541 catctgcata cgattgtgga aggtttgttc ggtttggtgc aggacattct ggagttcagt
      601 caaatcgctt cgtagtttgc gatctgtttc atttccagta atgagctgac tatcaatatt
      661 tttgagatgt tcgtttacgg cttccatctg gactttgagt tcccgaacgt cattgaccag
      721 tgaaataaca tcatctcgcg gatattttac ctcgcggcgg atttcctgga gcttggccca
      781 caaaaattga tgggcagcat gggctgggcg aaagaaagtc gctaacaggt agaaagccgc
      841 aaagtagtac ccggtttgac cttgagacca gtaggtcacg cccgcaatca ccgctgcagc
      901 gccgatatgc gctccaatcg ccgtgaccag tgtgatccat cgcatccgca gcaaatactt
      961 ttcgcgatct gcattgactg caatattgcg ctcacgcgaa agattcattt caaaaagcag
     1021 cgcccggatt tcaaagtgca aatcccaggg cactttcata attaccatca atccggtcag
     1081 gcacagaaac cccatgccga cattgagtaa caccgcacct gagacctgag gaagcggact
     1141 ccaatgaaac acccacgaca gcacacttaa aacgccaaac gccagacatc ccaacaggca
     1201 aagtcccagg atggcttcaa agaatttttt ggacatggtt tttggattcc tttcaactgt
     1261 gagtcactga tagggcggta gcctagccac ggcaggctag tggagagaat ggcgatatgg
     1321 cggtttattg tttgtcctat tcgaagggct gaaaacatcg ggctgaagaa tatcgggctg
     1381 aagacgtcgg gcttggggct gaagacgtcg ggcttggggc tcagggtata cagattattt
     1441 aaatgcaatt ggttttaccc tcagccccca gccctgagcc cgtcttcttc agcccgatat
     1501 tcttcagccc caagcccgac gtcttcagcc ccgagcttgc gagtcttcag cccgacgttt
     1561 tcagcccgac gtcttcagcc cctgttttct gaggtaaaaa ctgttcgaca aaggtccgca
     1621 attcggccag cgaggtatca gacaacacac ggaaaaccca ggctttacag tctgaaggga
     1681 tgagtttcgc tgttaatgcg tcagtgagca ggaagccaca ggcgcgcaag ccgcgattcc
     1741 agtctggttc gcgggcatcg cggaagttga gtgccagtga atcaacccct gctgccacaa
     1801 gtgtggtgtg tgcccagcgt aaaaactccg gccagtgtg
//
LOCUS       DAOQDO010001445         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65846_length_1838_cov_3.351655, whole genome shotgun sequence.
ACCESSION   DAOQDO010001445 DAOQDO010000000
VERSION     DAOQDO010001445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65846_length_1838_cov_3.351655"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..473
                     /locus_tag="PKZ53_19605"
     CDS             42..473
                     /locus_tag="PKZ53_19605"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNJ42703.1"
                     /translation="MSKHTASLFCLLFLCVCQVLVPQVSGNTTQGGNNIRSPQDNEEN
                     HPKHQTEAELNQAIEIAEREKYSVRRVEFLGNYTTRDRILRRELTFDEGYEFTRKKLE
                     ISLARLNRLKRFHPIKLRDVWVELDSKYKEIDVTIKVVEKK"
     gene            complement(501..1037)
                     /locus_tag="PKZ53_19610"
     CDS             complement(501..1037)
                     /locus_tag="PKZ53_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42704.1"
                     /translation="MEIILILVGGVILALGVGFAHQAVVAYRCRTLTLSQAIQNVADR
                     PTLQNQTIFVNKLIESELGLIILDSKTPINHLELITTRPGDDIRVATSTGPNGEPFLL
                     AVCDVPTYFDQHPEHPIAIVTPKTALELAIQGGFEGLIVQKGDDAWAGVLKSEVERLL
                     QLPKVKNQGPTMPGVFKK"
     gene            1377..1661
                     /locus_tag="PKZ53_19615"
     CDS             1377..1661
                     /locus_tag="PKZ53_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42705.1"
                     /translation="MTHEKFRLLFFIVLGIGITGWIQWSWEYPGIDSYLRGFPIPFYD
                     SDITGGGRGHPRFDIFFFILDVTLTGFICGLALLRLDSLIQKIFRSGQKI"
     gene            complement(1658..>1838)
                     /locus_tag="PKZ53_19620"
     CDS             complement(1658..>1838)
                     /locus_tag="PKZ53_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42706.1"
                     /translation="VGVEVEDALTWEDIVNGYICSGLVINEALWNAASHPGRFSVFKP
                     GYFWQPFRVRDGQLG"
BASE COUNT          507 a          439 c          412 g          480 t
ORIGIN      
        1 tgacactgct tttggtgctt tgagttacaa gaaaggtcca tatgtcaaaa cacacagcca
       61 gcttgttttg tcttcttttt ctctgtgtct gtcaggtcct ggtgccacag gtatcaggga
      121 atacaaccca gggagggaat aatattcgtt cccctcaaga caatgaagag aaccatccca
      181 aacaccagac tgaagctgag ttaaatcaag caattgagat tgcagaacgt gaaaaatatt
      241 cggtcagacg ggttgaattt cttggaaatt atacgacccg agatcgaatc ctccgccgag
      301 agttaacctt tgatgaaggc tatgagttta cgcggaaaaa actggagata tcgcttgcca
      361 gattaaaccg tttaaaacga ttccacccga tcaaattgcg tgatgtttgg gtcgaactcg
      421 attccaaata taaagaaatt gatgtgacca ttaaggtggt tgagaaaaag tagcaaccac
      481 acctggaaca aacaccagac ctattttttg aacaccccag gcatcgtcgg accctggttt
      541 ttcacttttg gcaattgaag taaccgctcg acctcagatt tgagcacacc agcccaggca
      601 tcatctcctt tctgaacaat caatccctca aacccaccct ggattgccag ttcgagggcg
      661 gttttgggag tcacaatggc aatcggatgt tcaggatgct gatcaaaata ggttggtaca
      721 tcacaaacag ccagcaaaaa gggttcacca ttggggccag tcgaagttgc cacccggata
      781 tcatctcctg gccgggttgt aatcaactca agatgattga taggagtctt tgaatccaga
      841 atgatcaatc caagttcgct ttcaatcagt ttattcacaa aaatggtttg gttttggagc
      901 gttgggcggt cggctacatt ttgaattgcc tgactgagtg tcagggttcg acacctgtac
      961 gccaccacgg cttgatgagc aaagccaact ccaagcgcaa gaatgactcc tccaacaaga
     1021 atcaggataa tttccatcaa tatctccttg tcttacccag gtttaaaccc accaaagaat
     1081 tgaagttttg agaaacatgt tttcaaaatt ccagggaaca agttgaagac agcctgtgtc
     1141 taacagcctt gaaccacaca aaggtatggc ttcttagaaa aagaaacctg gctggagcgc
     1201 tggtgtcctg ccggcaactg attaaagagc aaccaattga caccggcaag gggctgacgc
     1261 tcccaggaaa tatttatctg aaactctgta gctccggtca tgcaaatttg ttctttgccg
     1321 gagtttttca aacgcaatct gattgatgat tggcctgtct ggcgagggag acacctatga
     1381 ctcatgagaa atttcgcctg ctctttttta tcgtcctggg aatcggtata actggctgga
     1441 tccaatggag ttgggaatac cctggcattg attcgtacct tcggggcttt ccgattccat
     1501 tttatgattc agacatcact ggcggcggtc gcggccatcc acggtttgat atttttttct
     1561 ttatcctgga tgtgacattg accggtttta tctgcggtct ggctctgctc aggctggatt
     1621 cactcatcca gaaaatcttt cgatcaggcc agaaaattta gccaagctga ccatccctga
     1681 ctcgaaacgg ctgccagaaa taccctggtt tgaacacact gaaccggccc gggtgactgg
     1741 cagcattcca aagggcttcg ttgatgacca gtcccgaaca aatatacccg ttcacgatat
     1801 cctcccaggt gagagcgtct tcaacctcaa caccaacc
//
LOCUS       DAOQDO010001446         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65875_length_1838_cov_2.282109, whole genome shotgun sequence.
ACCESSION   DAOQDO010001446 DAOQDO010000000
VERSION     DAOQDO010001446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65875_length_1838_cov_2.282109"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..454)
                     /locus_tag="PKZ53_19625"
     CDS             complement(86..454)
                     /locus_tag="PKZ53_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42707.1"
                     /translation="MFADLLRSIIGRVEGSLGALIMGLDGIAVEQVEVQKLEKSEALN
                     MVAAAHATLLRNSMRLTSDVGVGALNELTVMSSDMTLVMKLINREYFLLIAIRPDGNL
                     GRARFELRKAQLQIAEEFSI"
     gene            complement(454..>1838)
                     /gene="pilQ"
                     /locus_tag="PKZ53_19630"
     CDS             complement(454..>1838)
                     /gene="pilQ"
                     /locus_tag="PKZ53_19630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNJ42708.1"
                     /translation="DIKVTLKVTDVPWNQVLEAILRSNRLGYQTEGKLIRIATLTAFA
                     EEEKQRTDRAEAARNNIPMITEYFKLKYERVLGGQLGGGGGAAAAGGAGGGGGGGTGP
                     GSIGGVGLRPIVSQTLSQRGSISVNPRTNMLIVTDIPERVEKIRQIIARLDVAEPQVE
                     IEARIVQADRTFLRDLGVQLFTGVVARRGGGVGFSTSAGQSYGLGFGGDAGAGSGIIR
                     GTYTPRNFVTDSLFIGPLASGAIGNLNPASSVLSLTTGLIGTGFISAALTAAESKGIA
                     KQISSPRVTVQNNSEGEIKSGVSIPYTAAQAIGATAIATTTFQEATLSLKITPQITVE
                     GNVLLSLESTNDSVNQSVPVNGQPAINRQSSKTLVLVPDGGTTIIGGVNVDRESDSVN
                     RTPGISSIPLLGNLFKQRSVRRQTTELLFFITPRIFRGENFITNGVPPTPVPAPSPAG
                     VDQSTVGGQP"
BASE COUNT          466 a          495 c          425 g          452 t
ORIGIN      
        1 ttcgaccgta acttttctcc caaaagacaa aaaaaacccc ctactttaga atctaaagta
       61 gggggttttt tgggaggtat tcgcgtcaaa ttgaaaactc ttctgcaatt tgaagctgag
      121 ccttacgtaa ctcaaagcgg gctcttccca ggtttccatc gggccgaatc gcaattaaga
      181 gaaaatactc tcgattgatc agtttcataa ccagagtcat gtcgctggac ataacggtta
      241 gctcattgag agcgcctacg ccaacatctg aagtaagccg cattgaattt cgaagtagcg
      301 tcgcatgcgc ggcagccacc atgttgagcg cctcagattt ttccaatttc tggacttcga
      361 cttgctcaac tgcaatgccg tccaacccca tgatcaatgc gccgaggctt ccttcgactc
      421 tcccaataat tgacctgaga agatctgcaa acattacggc tggcctccga ctgttgactg
      481 gtcaacgcca gccggtgaag gtgcaggaac cggggttggt ggaactccat tggtaatgaa
      541 gttttcacca cggaaaatac gtggggtgat gaagaacagc aattccgtgg tttggcgtcg
      601 gacggagcgt tgtttaaaca agttccccaa taacggaatg cttgaaattc caggggttcg
      661 atttacggag tcactttcac gatcaacgtt gacaccaccg atgatggttg tcccaccatc
      721 tggtaccaac accagcgttt tcgagctttg ccggttgatc gcaggttgac cattgactgg
      781 aaccgactgg ttgacgctgt cattggtgga ttcaaggctt aacagcacat tcccttcaac
      841 agtgatctga ggtgtaattt tgagactcag ggttgcttcc tgaaacgtgg tggtggcaat
      901 cgccgtcgcc ccaatcgcct gggctgccgt atatggaatg gagaccccag atttgatttc
      961 gccttctgag ttattttgga cagttactct aggggacgaa atctgcttgg cgattccctt
     1021 tgactcagcg gcggtgagcg cggctgaaat aaagcccgtt ccaatcaaac cagttgttaa
     1081 gcttaacact gaactggctg gatttaagtt accaattgct ccagaggcca gcggaccaat
     1141 aaacagacta tcggtcacga agtttcgcgg agtatatgtc ccacgaataa ttcctgatcc
     1201 agcacccgcg tcccctccaa aacccaagcc ataggactgt cccgctgagg ttgaaaagcc
     1261 aaccccacca cctctacggg ccacaacccc tgtgaataac tgaacaccca aatcgcgcag
     1321 gaaggtccga tccgcctgaa caatgcgggc ttcgatttca acttgaggtt cagccacgtc
     1381 gagccgggca atgatttgac ggatcttttc aactcgttca ggaatgtcag tgacaatcag
     1441 catgttggta cgtggattca ctgaaattga accgcgctgg gacagtgtct gagaaacaat
     1501 cggacggagt ccaaccccac caatgctccc aggtccagtt ccaccaccac caccaccgcc
     1561 agcaccacca gccgccgcgg caccaccacc accacccagt tgaccaccca gcactcgctc
     1621 atatttaagt ttaaagtact cggtaatcat tggtatattg tttcgggcag cctcggctcg
     1681 atcagtgcgt tgtttttctt cttctgcaaa cgcagtcaat gtcgcaatgc gaatcaattt
     1741 tccttcggtt tggtatccca accgattgga acgcaaaata gcttccaaaa cctgattcca
     1801 cggaacgtca gtgactttca atgtcacttt gatatccc
//
LOCUS       DAOQDO010001447         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65931_length_1837_cov_2.643098, whole genome shotgun sequence.
ACCESSION   DAOQDO010001447 DAOQDO010000000
VERSION     DAOQDO010001447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65931_length_1837_cov_2.643098"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1837)
                     /gene="drmD"
                     /locus_tag="PKZ53_19635"
     CDS             complement(<1..>1837)
                     /gene="drmD"
                     /locus_tag="PKZ53_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HNJ42709.1"
                     /translation="DRRQLQAVMVRRLKRELPARWDGTPRFPERKLEALSVAYTEQEQ
                     RIHRALKAYTASRTSEVSDAIERYATEFVLKLLKKRLFSSPAAFITTLDRHELSLKES
                     RRRKSAPIRPSQGILKAFLGKIDEDYADDDAYEEATADAVTITSPLFRPPTAEETGYL
                     KEMRDWAEQASVVADSKAHTLLSWLETNLRPNHHWTDNRVIIFTEYRATQKWLHQILA
                     NEGYARDERLMLLFGGMDAQDRERVKAAFQTAPDISPVRILLATDAASEGLDLQNFCS
                     NLIHYEIPWNPNRMEQRNGRIDRHGQRASEVKVFHFVGSQFRSTTPSERVGDIDGDLE
                     FLFRAALKVETIREDLGKVGPVIAQQVEEAMLGHRHRLDTSRAEQESESVRRLFKFER
                     KLREQIAEFRDQLYETRQPLRLSPENIRDVVLIALELAGQPPLAPVSVPGLAGPAYRV
                     PGFKGSWSACLEGLAHPHTLKIRPIVFDPELAIGRDDVVLAHLNHRLVQMCLRLLRAE
                     VWSSGSTRRLNRVTARVATSPVLESPAVVAYGRLVVLGGDQQRLHEEVIVAGGIIREG
                     RFNRFNVGQTQAVLETVTDELVPAGVHRQLAGLWPTIGPGLLAALE"
BASE COUNT          386 a          503 c          544 g          404 t
ORIGIN      
        1 gttcgagtgc tgccagcagc cctggcccaa tggttggcca taacccggct aattggcggt
       61 ggactcccgc tggaacaagt tcatcggtca cggtttcaag cacggcctgg gtttgaccaa
      121 cgttgaaccg gttaaatcgg ccttcacgga tgattccccc ggctacgatc acttcttcgt
      181 gtagccgttg ttgatcacca cccagaacga ccagccggcc ataggccacc acggcaggcg
      241 attcaagcac tggagacgtg gcgacgcggg cggtaacccg gttgagccga cgggtgctgc
      301 cggacgacca gacttcagcc cgcagtaatc gcaaacacat ttgaaccagc cggtgattga
      361 gatgagccag caccacatca tcgcgaccga tggccagttc ggggtcaaac acaatcggac
      421 ggatcttgag tgtgtgcgga tgggccaacc cttccaggca ggctgaccag cttcccttga
      481 acccaggcac ccgataggcc ggtccagcca aacctggaac cgaaaccggt gccaacgggg
      541 gttgcccggc cagttccagc gcaatcaaga ccacatcccg aatgttctct ggggacagac
      601 gcaggggttg tctggtttca tacaactggt cacgaaactc agcgatttgc tcacggagct
      661 tgcgttcaaa cttaaagagc cggcgcactg attctgattc ttgctctgcc cgggaggtgt
      721 ccaggcggtg acggtgaccc aacatggctt cttcgacctg ctgggcaatc accggcccga
      781 ctttgcccag gtcttcgcgg atggtttcaa ctttgagagc ggctcgaaac agaaattcga
      841 ggtcgccatc aatatctccg acccgttcgg atggagtcgt actccgaaac tggctgccga
      901 caaaatgaaa caccttgact tcactggccc gctggccgtg gcggtcaatc cgtccattgc
      961 gttgttccat ccggttggga ttccagggga tttcatagtg aatcaagttg gagcaaaaat
     1021 tttgcaggtc gagcccttca gaggccgcgt cggtcgccag caaaatccga accggcgaga
     1081 tatcaggcgc ggtttgaaag gcggccttga cccgttcgcg gtcctgggca tccatcccgc
     1141 caaagagcaa catgagccgt tcatcacggg cgtagccttc attggccagg atttgatgca
     1201 gccatttctg ggttgcgcga tattcggtga aaatgatgac ccggttgtca gtccagtggt
     1261 ggttgggacg gagattggtt tccagccagg ataaaagcgt gtgggctttg gaatctgcca
     1321 caaccgaagc ctgttcggcc caatcccgca tttctttgag gtagccggtt tcctcggcag
     1381 ttggaggccg aaacagcggg gaggtgatgg tgacggcatc ggcggtggct tcttcatagg
     1441 cgtcatcatc cgcatagtct tcatcaattt ttccaagaaa tgctttgaga atgccctggc
     1501 tgggtcggat cggggctgat ttacgccgtc gggattcctt gagcgaaagt tcgtggcgat
     1561 caagggtggt aataaaggct gccggtgatg aaaagagccg ctttttgagc agtttgagga
     1621 caaactcagt ggcatagcgt tcaatggcgt ccgacacctc tgaggttcgg gatgcggtgt
     1681 atgctttcag tgcccgatgg atgcgttgtt cctgctcagt gtaggcgaca gaaagggcct
     1741 ccagtttccg ttcgggaaac cggggtgtcc catcccatct ggcgggaagt tcacgtttga
     1801 gccgtcgcac cataacggct tgcaactggc gccggtc
//
LOCUS       DAOQDO010001448         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65948_length_1837_cov_2.328844, whole genome shotgun sequence.
ACCESSION   DAOQDO010001448 DAOQDO010000000
VERSION     DAOQDO010001448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65948_length_1837_cov_2.328844"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1605
                     /locus_tag="PKZ53_19640"
     CDS             220..1605
                     /locus_tag="PKZ53_19640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42710.1"
                     /translation="MSTETLNNILFPILAQTDIDCLIPYGSVITLEPGDVFFHEGEPF
                     GYFAIVLDGEIKISKQAAGGEIVLTVHEPGGFVGEIGLMTRNPAIATARATKKSQLLR
                     LEVGVLRELLNACPTLSEVILPALIRRRPEADLLTQQREKMAALGKLSAGLAHELNNP
                     AAAAKRSAGYLQQTLNRLNESFFNLNSHGLTADHWTTIKGFLADVNAAKLQTRQLDSL
                     TQSDLESELGDWLEEQNIPDGWNLASTLVQAGLGREPLSRFLDELPRSSQADVLVWLE
                     ASIQAGELSREIEHSASRISTLVKAVKSYSYMDQSPVQEIDLHESLDTTLTILNHKLR
                     SGISVVKEYRCTVERIVGYGSELNQVWTNLIDNAIDALGGQGCLWLRTWCQADLVVVE
                     IVDNGPGIPADIRSRIFEPFFTTKQVGQGSGLGLDIAYRIVRRHGGDILVDSQPGNTS
                     FTIRLPIGFTP"
BASE COUNT          425 a          481 c          485 g          446 t
ORIGIN      
        1 acaagggact tgccagtcag aagtcaggag ttcgctcagt ttgtttggtt gggtcctgtg
       61 actgttttct aatccggggg cgccactgca atatggctat cacctgaccg aagctattca
      121 atcaactgct cattccgctg gtacgcatct ggaacgtgaa tcaattcggt tcagttcctc
      181 ccttgaacct cgaaccccga atcctcaatg gtattttcaa tgtcaactga aaccttaaac
      241 aacattttgt tcccgatact tgcgcaaacc gatattgact gcctgattcc ctatggctcg
      301 gtcataacgc ttgagccagg tgacgttttt tttcacgaag gtgaaccatt tgggtacttc
      361 gctattgttc tggatgggga gatcaaaatt tcaaagcagg cggctggtgg tgagattgtc
      421 ctgacggttc acgaacccgg cggctttgtc ggagaaatcg ggctcatgac gcgcaatcca
      481 gccattgcca ccgccagagc gacgaaaaaa agccagcttc tcagactcga agtgggggtg
      541 ctccgtgaac ttctcaatgc ctgtcccacc ttgtcagagg tcatcttacc agcgttgatc
      601 cgtcggcgac cagaagctga cctgctcacc cagcaacgcg aaaagatggc ggcgctcgga
      661 aaactctccg ctggattggc ccacgagtta aacaacccgg cggcagcggc aaaacggtca
      721 gccggttatt tgcaacagac gctcaaccga ctcaacgaaa gctttttcaa cctgaattca
      781 cacggcctta ccgctgacca ctggacaacc atcaagggtt ttttggctga cgtcaacgcg
      841 gccaaacttc agacccggca gttggattca ctgacccaaa gtgatctgga atctgagttg
      901 ggtgactggt tagaagagca aaatatcccg gacggctgga atctggcctc aactttggtc
      961 caggccgggt tggggcgtga accattgagc cgatttcttg acgaattgcc cagatcatcc
     1021 caggctgatg ttctggtgtg gcttgaagcc tcaattcagg cgggtgaact cagtcgcgaa
     1081 attgagcatt cggccagtcg catttcaacc ctggtcaaag ccgtgaagtc ctattcctat
     1141 atggatcaat ctccggttca ggaaattgac ctgcacgaaa gcctggatac caccttgacc
     1201 attttaaatc ataaactacg gtctggaatc agcgtggtga aagaatatcg gtgtacggtt
     1261 gaacgcattg tcggatatgg aagcgaactc aaccaggtct ggaccaattt gattgataat
     1321 gcgattgatg cactgggcgg gcaaggctgt ctctggcttc ggacctggtg tcaggcagat
     1381 ctggttgtgg ttgaaattgt tgataatgga ccgggcattc ccgccgacat ccgctcccgc
     1441 atctttgaac cctttttcac caccaaacag gttggccagg gaagtggcct gggactcgac
     1501 attgcctacc ggattgttcg tcggcatggg ggcgatatcc ttgttgattc ccagcctgga
     1561 aatacatcat ttaccatccg gctgccaatt gggtttaccc cataagggca atggcattaa
     1621 gttaagggtg ggagcgcggg catcctgccc gcttggattt caattccaac cgactgcggg
     1681 cgagacgccc gcgctcccag gtgaagatat tttgcttaac ttaataccat tgcccctgcg
     1741 gggcgcgggc gatgtgggga gggattgggg agatggcctt tccccggtgg tagctcccaa
     1801 aacctcgcaa ccaccggcta tccgaacggc agccttt
//
LOCUS       DAOQDO010001449         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_65958_length_1837_cov_2.212682, whole genome shotgun sequence.
ACCESSION   DAOQDO010001449 DAOQDO010000000
VERSION     DAOQDO010001449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65958_length_1837_cov_2.212682"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKZ53_19645"
     CDS             <1..377
                     /locus_tag="PKZ53_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HNJ42711.1"
                     /translation="SLNCVLAQRLVRQLCTHCARTYQPTDIELIESALDPDKCRQYQF
                     YEHVGCEHCNYTGYRGRTAIHELLDLSDHIRELILDRRPGSEIRRAARQEGLTSLRET
                     AVQKVFRGLSTLREINRVTFIE"
     gene            447..>1837
                     /locus_tag="PKZ53_19650"
     CDS             447..>1837
                     /locus_tag="PKZ53_19650"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNJ42712.1"
                     /translation="MNQRCSFRYRLYRAACHLLCLLLVLNLFGGSTALAFNGKKYFKT
                     GQKYEATKEWDRAAEEFSLALREDPGNAEYELHYLRCVSNASLMFMDRARMLVAQGDY
                     ESAYMAYKQAFTYDPTNEQAQAKMKEMLMKQGITPLPNGDPDPYQKVSEKNKQVMDYV
                     APASPKIADSIVITERSIEDIILQLASFINFNVVFDAQAKQPVTQKSKLSLKNITYAE
                     ALEIVLDANNLAFYPVGRRTILVGPKNNPQLAARYDTSLLKTFYIRNAKLQDVQTLIT
                     QMLGAGKQVAQNTQNNTLTVRDSPENLDIIQKLIASIDKPQAEVVMDINIYEVSQDDL
                     LQIGNQFNTGTGRNQASLGNLGGIAQSGLRNSSATGIFGPLGTALVLPPSTLSFLQSR
                     GNTRLLAQTQLHSFESEEATVNVGQKVPTQTASIPTFNNNNNNNNNNNNNNNNLFGGI
                     NQVQYVDVGLNIS"
BASE COUNT          510 a          476 c          417 g          434 t
ORIGIN      
        1 gttcgctcaa ctgtgtactg gcccaacgcc tggttcgcca actctgtacc cactgtgccc
       61 gaacctacca gccaaccgat atcgaactga ttgaatcggc gctggatcca gacaagtgcc
      121 gccagtacca gttttacgag cacgttggct gcgaacactg taactacacg ggatatcgtg
      181 gccgaacggc catccacgag ctgctggatt tatctgacca cattcgtgaa ttgatcctgg
      241 atcggcgtcc tggttcggaa attcgccggg cagcccgcca ggaagggctc acttcgctgc
      301 gtgaaaccgc agttcaaaaa gtttttcgag gattatcaac gctacgcgag atcaaccgcg
      361 tgacgtttat tgagtagagg cgactcacca tttttgtttg atcttgagat gactcactat
      421 atttttgttt ggttttgagg aaagttatga atcaacgatg ttcttttcga taccgactgt
      481 accgtgcagc ctgtcacctg ctttgtctgc tgctggtttt gaacctgttc ggtggcagca
      541 ccgccctcgc ctttaacgga aaaaaatatt ttaagaccgg gcagaaatat gaggcgacca
      601 aagaatggga tcgagcggct gaagaattca gcctggcact gcgcgaggat ccaggaaatg
      661 ctgaatacga attgcactat ctgcgctgtg tctcaaatgc gtcgttgatg tttatggacc
      721 gggcccggat gctggtcgca cagggagatt atgaaagtgc ctacatggcc tataagcaag
      781 cctttaccta tgatccgacc aatgaacagg cccaggccaa aatgaaggaa atgctgatga
      841 aacaggggat taccccttta cctaatggcg accctgaccc atatcagaag gtttccgaaa
      901 aaaacaaaca ggtgatggat tatgtggcgc cagcctcacc caaaattgcc gacagtattg
      961 tgattactga gcgctcaatt gaagatatta tcttgcaact ggcttcattt attaacttta
     1021 atgttgtctt tgatgcccag gccaaacagc ccgtcaccca gaaatcaaaa ctctcactca
     1081 aaaacatcac gtatgccgag gcattagaga ttgtactgga tgccaataac ctggcgtttt
     1141 atccggtcgg gcggcgaaca atcctggtcg gccccaaaaa caatccgcaa ctggccgcac
     1201 gctatgacac cagcctgctc aaaaccttct atatccggaa cgccaaattg caggatgttc
     1261 aaacgctgat cactcaaatg ctgggtgctg gcaaacaagt cgcccagaat acccaaaaca
     1321 acaccctcac cgtccgtgac tcgccggaaa atttggacat tatccagaaa cttattgcca
     1381 gtattgacaa accccaggca gaagtcgtca tggatatcaa tatctatgaa gtctcacaag
     1441 acgacctgct gcaaattggg aatcaattca acactggcac aggtcgaaat caggcatcgc
     1501 ttgggaattt aggtggtatc gcccaaagtg gactgcgcaa ttcgtcagcc acggggatct
     1561 ttggccctct gggtacagca cttgtccttc cgccatcaac gctgagtttc ctgcaatcgc
     1621 gtggaaatac tcgattgctg gcccaaaccc agttgcattc gtttgaaagc gaagaagcca
     1681 ccgtgaatgt cggccaaaaa gtcccaactc aaacggcatc gatcccaacc ttcaacaaca
     1741 acaacaacaa caacaacaat aacaataaca acaataacaa cctttttggt ggaatcaatc
     1801 aggttcaata tgttgacgtt ggcctcaata tttcgat
//
LOCUS       DAOQDO010001450         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66009_length_1836_cov_2.435149, whole genome shotgun sequence.
ACCESSION   DAOQDO010001450 DAOQDO010000000
VERSION     DAOQDO010001450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66009_length_1836_cov_2.435149"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKZ53_19655"
     CDS             <1..376
                     /locus_tag="PKZ53_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNJ42713.1"
                     /translation="FDLELNLVALKEMSSDVRSVRQFVDEYVITETGNRVIVLGEGRL
                     VNLAAAEGHPASVMDMSFANQALSAEYLKKNEGKLANQLHVLPGEVDQEIASLKLEAM
                     GVKIDTLTPEMVEYINSWELGT"
     gene            complement(590..1501)
                     /locus_tag="PKZ53_19660"
     CDS             complement(590..1501)
                     /locus_tag="PKZ53_19660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNJ42714.1"
                     /translation="MTERIRIFITGASGFVGTHLIRALTANQIEVIGLSRGKGRSEGL
                     VRWVQGTSSEPGDWQKEIDGCHGVINLAGEPVVGKRWSPEVKQRIRDSRVKTTSCVVD
                     AIERAKMRPSVLISASAVGLYPKNLETAMDENTPPADDFLGEVCQEWERAAQRVESLK
                     VRLVLLRIGVVLGLEGGALAQMMTPFKFGVGGPVGGGKQWFPWIHIDDVVEMIVFSLN
                     NGQVRGPVNCVAPNPVRMGEFTSELGRALHRPAILPVPGFAVQLLFGESASVVLDGQR
                     VTPAVAEKVGYKFRFPKLDEALRNLVG"
BASE COUNT          470 a          542 c          405 g          419 t
ORIGIN      
        1 ctttgacctg gaattgaacc tcgtggcgct caaggaaatg tcatctgacg tgcgttcagt
       61 ccgacagttt gtggacgaat atgtcatcac cgaaaccggc aaccgcgtca tcgtgctcgg
      121 cgaaggccgt ctggtcaatc tcgccgccgc cgaaggccat ccggccagcg tgatggatat
      181 gagctttgcc aatcaggcgc tctctgccga atacctcaag aaaaacgaag gcaaactggc
      241 caaccagttg cacgtgctgc ccggtgaagt tgaccaggaa attgcttcgc tcaaactcga
      301 agccatgggt gtcaaaattg ataccctgac cccagaaatg gtcgagtaca tcaatagctg
      361 ggaactcggc acctaaagca ggaatgaaga attgagaatg aagaatgaag aatgaagaat
      421 gaagagtgtg cctggttttt cttcgaaagt attgattcct aaaaatcaag aaccagtcac
      481 cacgtacgaa gcaacagtca tcagcaatga ccagctacct taactttctt cattctcaat
      541 tctcaattct tcattctcaa ttctcaattc tcaattcttc attcttcatt catccgacca
      601 gatttctgag ggcttcatcg agtttcggaa atcggaattt gtagccgacc ttctccgcca
      661 ccgccggcgt cacccgctga ccatccagaa ccacgctggc agattcacca aacaaaagtt
      721 gtacggcaaa tcctggcacg ggcaaaatcg ctggacgatg cagtgcccgg cctaattcag
      781 acgtaaattc tcccattcgc accgggttgg gggcgacaca attgaccggt cctcgaacct
      841 gaccattgtt caagctaaac acaatcattt caaccacatc gtcaatatga atccagggaa
      901 accattgttt cccgcctccg accggcccac caacgccaaa cttaaatggc gtcatcattt
      961 gcgccagggc gccgccttcc aaccccaaaa ccactccaat tcgaagcagc accagtcgca
     1021 ctttgagcga ttcaacccgc tgtgccgcac gctcccactc ctgacatact tcgcctaaaa
     1081 aatcatctgc cggaggggtg ttctcatcca tcgccgtttc caggtttttg ggatacagac
     1141 caacggcgga agctgaaatc agtacggaag gacgcatttt ggcccgctcg atggcatcca
     1201 caacacaact tgtcgttttc actcgactat cccgaatgcg ctgtttgact tcaggactcc
     1261 agcgttttcc aaccacgggt tcaccagcga gattgatcac gccgtggcac ccgtcaattt
     1321 ccttttgcca gtcacctggc tcactggatg ttccctgaac ccaccgcacc agcccctcac
     1381 tgcgaccttt ccctcggctt aacccaatga cttcaatctg gttagcagtc agggcgcgaa
     1441 tgaggtgtgt tccaacaaac cccgaagccc cagtgataaa aatccgtatg cgttcggtca
     1501 tagtaaccat ccttctggtg aattttcttg caaagaattg aatcggcttg gattatactc
     1561 ccggtcgccc aaacaagcct gagtgttttc cgattcaggg ctgagaaacc cagggctgag
     1621 aaacccaggg ctgaagaaag cgggcttggg gctgaagact cgcaagctca gggctgaaga
     1681 catcgggtgt aaaaagcctt cagcccgtcg tcttcagccc gtcgtcttca gcccgtcgtc
     1741 ttcagcccgt cgtcttcagc ccgacttctt ccgccctcaa aaaatatgac tgaaacttta
     1801 gatctcccgt cacgcaagcc aacaccagac agttca
//
LOCUS       DAOQDO010001451         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66042_length_1835_cov_3.650000, whole genome shotgun sequence.
ACCESSION   DAOQDO010001451 DAOQDO010000000
VERSION     DAOQDO010001451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66042_length_1835_cov_3.650000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKZ53_19665"
     CDS             complement(<1..274)
                     /locus_tag="PKZ53_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNJ42715.1"
                     /translation="MTAPLPASFGSWKSPISADLIVAKTINLLEIHVSGSDIYWIEGR
                     PLEAGRSVLVKWNGDSTTTDLIPEGFNARTRVHEYGGAPVLVDGETV"
     gene            complement(303..1226)
                     /locus_tag="PKZ53_19670"
     CDS             complement(303..1226)
                     /locus_tag="PKZ53_19670"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNJ42716.1"
                     /translation="MKDLLGIRPLNPQMILDVLDRADGFLPLETPDQATLSRCAGRTL
                     VNLFFEASTRTRTSFELAAKRLGMEVVNLVVGASSVTKGETLRDTILTLDAMRPDAVV
                     VRHSTSGVPAHLANFSQAHFINAGDGAHEHPTQALLDALTIRRHFGKIAGLTVAILGD
                     VLHSRVARSNVHLLTKLGAQVRISGPRTLLADGIEHLADGNAGTATVYTSIQEAVTEA
                     DAIMMLRIQRERQNSAFLPTLGEYFKFYGLNQTRLQLARPNAIVLHPGPINRNVEIAS
                     EIADSVQSKILEQVQNGVAVRMAVLEKLLHS"
     gene            complement(1262..1810)
                     /gene="pyrR"
                     /locus_tag="PKZ53_19675"
     CDS             complement(1262..1810)
                     /gene="pyrR"
                     /locus_tag="PKZ53_19675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNJ42717.1"
                     /translation="MEFIEKVRLMDAPQMNRVLARMASEIVERNHGIDDLLLTGIRRR
                     GVPLAERMADRITELEGTRPPVGILDITLYRDDLTLVASAPVVNQTEMPEDITPYTIV
                     LVDDVLYTGRTIRAALDALFHIGRPKRVQLAVMVDRGFRELPIHADYIGEKVATKASE
                     IVKVMIDSIDGINQVLIVEAKP"
BASE COUNT          430 a          466 c          503 g          436 t
ORIGIN      
        1 acactgtttc gccatcaacg agcactggcg cgccgccgta ttcgtgaacc ctggttcggg
       61 cgttgaagcc ttcaggaatc aaatcagtgg ttgtgctgtc accattccat ttgaccagga
      121 cgctgcgacc tgcctcaagc gggcggcctt ctatccaata aatatcagag ccagaaacgt
      181 ggatttcaag caggttgatg gtcttggcga caatcaaatc agcggaaatt ggggatttcc
      241 aggaaccaaa agaggctggg agtggagccg tcatatatca aaatgatctt tttgggttgg
      301 tttcaggagt ggagtaattt ctcaagcacg gccatgcgca cggccacacc gttttgaacc
      361 tgctccagga ttttggattg caccgaatca gcaatctcag aggcgatttc cacattgcgg
      421 ttgattggac cagggtgcaa aacaatcgca tttgggcgag ccagttgaag acgggtttga
      481 ttgagaccgt aaaacttgaa gtattcaccc agggttggca ggaaggcact gttttggcgt
      541 tcgcgctgga ttcgcagcat catgatggca tcagcctcag tcaccgcctc ttgaatggag
      601 gtataaaccg ttgctgttcc agcatttcca tccgccaggt gctctattcc atcggccagt
      661 aacgttctcg ggccactgat tcgaacctgg gccccgagtt tggtcagcaa atggacattt
      721 gagcgagcga cccgactatg gaggacatct cccagaatcg ctacggtcaa cccggcgatt
      781 ttcccgaaat gtcgtcgaat cgtgagcgcg tcaagcagtg cctgggtggg atgttcatgc
      841 gcaccgtctc cggcattgat gaaatgtgcc tgtgaaaaat tcgccaaatg tgccggtaca
      901 cctgaagtgg agtgacgaac caccaccgca tcaggtcgca tggcatccag agtcaaaatg
      961 gtatcgcgga gtgtttcgcc tttggtgacg cttgatgcac caacgaccag attgacgact
     1021 tccattccaa gtcgtttggc agcgagttca aatgaggtgc gggtgcgggt tgaggcttca
     1081 aaaaacaagt tcacgagcgt ccgaccagca cagcgtgaca atgttgcctg gtctggtgtt
     1141 tcgagtggaa gaaacccatc cgcccgatcc agcacatcaa ggatcatctg ggggttgagc
     1201 ggacgaatgc caagtaagtc tttcataatg tggagtgcgg cggctcgacg ccgcttttca
     1261 gttacggttt cgcttcaact atgagcacct gattgatacc gtcaatgcta tcaatcatga
     1321 ctttgacgat ttcgctggcc ttggtggcca ctttctcccc aatgtagtca gcatggatcg
     1381 ggagttcacg aaatccacgg tcaaccatga cggcaagctg gacgcgtttc gggcgaccga
     1441 tatgaaagag cgcatcgagt gccgcccgaa tcgtccgtcc ggtataaagc acatcgtcaa
     1501 ccaggacaat cgtgtacggg gtaatgtctt ccggcatttc cgtctggttt accaccggag
     1561 cactggccac cagcgtcaga tcgtcccgat agagggtaat gtccagaata ccaactggag
     1621 gccgggtgcc ttcaagttca gtaatccggt cagccattcg ttcggcgagt ggaacgccac
     1681 gtcgtcgaat tccagtgagc aacagatcgt caattccatg gttgcgttcg acaatttcag
     1741 aagccatgcg ggccaggacg cggttcattt ggggcgcgtc catcagacgg actttttcaa
     1801 tgaattccat aagaatgtct cgggtttttg agtca
//
LOCUS       DAOQDO010001452         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66117_length_1834_cov_2.966273, whole genome shotgun sequence.
ACCESSION   DAOQDO010001452 DAOQDO010000000
VERSION     DAOQDO010001452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66117_length_1834_cov_2.966273"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1834
                     /locus_tag="PKZ53_19680"
     CDS             <1..>1834
                     /locus_tag="PKZ53_19680"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNJ42718.1"
                     /translation="KFTASYNPKKWSGGIKADEISTAGTLSPDWMDGGTQRDTGYKLD
                     NRTYSSRVILTTNDVTNIGVPFEWEAGTTKLSSVQKTLLNDGGHGENRVNYLRGQRTY
                     ENSTGTPYFRIRDSVQGDIVNSTVWFVDKPVSNYTDSSYKSFAKTHAKRFGMVYVGGN
                     DGMLHGFKAADGEELIAYIPKAVIPELTKLSDPGYTHQYYVDGSPFTGDVNIGNPSTN
                     DWRTLLVGALGAGGKGYFILDVTNPGNATGSPSSNFSTGNANNLVVMDKTIHPSAPLT
                     SGSDDEDIGHIFAAPVMDDNNVFKSAQISRMNDGRWAVVLGNGYNSKNERPVLLIQYL
                     DGAKELKKIVATGNQTLTGVPANDVNVTGNGLSAPKPVDINSDGKIDVVYAGDLKGNL
                     WKFDLTSDVASDWSVATWNNSTATPCNQATTKCQPMFTATYINGKRQPISAPPIVKPN
                     DRGAGGVMVAFGTGVNVTDTDRISTDVQSVYSLLDNTRYKQLTNKLIVNTDTSPAENG
                     VVPESITGRTNLVQQTIGTQHSGTGNSASFNFWEMSQNTVNYNAALGTTKQGWYFDLP
                     VSSERVLRQMSFFDGSNNLMVYSLTPAYGGTGSNEESCEPAGTPE"
BASE COUNT          522 a          451 c          450 g          411 t
ORIGIN      
        1 gaaatttact gcgagttata atccaaaaaa atggtctggc ggaatcaagg cagatgaaat
       61 ttccaccgca ggcaccctga gtccagactg gatggatggt ggaacacaac gggacactgg
      121 ctacaagctg gataaccgca catattccag cagagtgata ttgactacca acgatgtgac
      181 caacattggt gttccattcg aatgggaggc tggcacaacc aagctaagtt ccgtacaaaa
      241 aactttgctc aatgacggtg ggcatggtga aaaccgtgtc aactacctcc ggggccagcg
      301 aacctatgaa aacagcaccg gcacacctta ttttagaatc cgggattctg tgcaaggcga
      361 tattgtcaac tcgacggtat ggttcgttga caaacctgta agcaactata cggacagcag
      421 ttacaagtca tttgccaaaa cccatgccaa gcgcttcggc atggtttatg ttggaggcaa
      481 tgatggaatg ctgcacggct tcaaggccgc agatggcgaa gaattgattg cctatatccc
      541 caaagccgtc attcctgaat tgacaaagct gagcgatcca ggatacaccc accagtatta
      601 tgtagatggt tcaccgttta caggcgatgt caatattggc aatcccagca ccaacgattg
      661 gagaacgctt ctggttggcg cacttggcgc aggtggaaaa ggttatttca ttctcgacgt
      721 caccaatcca ggaaacgcta ccggctcacc atcctccaat ttttcaactg ggaacgccaa
      781 taatttggtg gtgatggaca aaaccattca ccccagcgcg ccgctcacat ccggctcgga
      841 cgacgaggac attggacaca tttttgcggc gcctgtcatg gatgacaata atgtcttcaa
      901 gagcgcacag atcagccgca tgaatgacgg acgctgggca gtggtgcttg gtaatggcta
      961 caacagtaaa aatgaacgcc cggtgctgct gattcaatac ctggatggcg ccaaggaact
     1021 caagaaaatt gttgccacag gcaatcaaac attaactggc gttcccgcca acgatgtgaa
     1081 cgtcaccgga aacgggctct ctgcccccaa accagtggat atcaacagcg atggaaaaat
     1141 cgatgtggta tatgcgggag atctgaaagg aaatctctgg aaatttgatt tgaccagtga
     1201 tgtggctagc gattggtctg tggccacctg gaataattca actgcgactc cgtgcaacca
     1261 agccactaca aagtgtcagc cgatgttcac tgcgacttac atcaacggca aacgacaacc
     1321 tatctctgca ccacccattg tcaagcccaa tgaccggggt gcaggtggcg tgatggttgc
     1381 atttggtacg ggcgtaaacg tgacagatac agatcgcatc agtaccgacg ttcagagcgt
     1441 ttattcactg ctggacaaca cgcgctacaa gcaactcaca aacaagttga ttgtgaacac
     1501 tgacacatct cctgcagaga acggtgtggt tcccgagtcc atcacaggtc gcactaactt
     1561 ggttcaacag acaataggaa ctcagcattc aggaaccggg aacagtgcaa gtttcaattt
     1621 ttgggaaatg tctcaaaata ctgtcaacta caatgcggcg ttgggaacta caaagcaagg
     1681 atggtatttt gatctccctg tttccagtga acgtgtatta cgccaaatgt ctttctttga
     1741 cggcagcaac aatttgatgg tttattcgct cacgcccgca tacggcggca ctggcagcaa
     1801 tgaagaatct tgcgagcctg caggaacacc tgaa
//
LOCUS       DAOQDO010001453         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66121_length_1834_cov_2.918494, whole genome shotgun sequence.
ACCESSION   DAOQDO010001453 DAOQDO010000000
VERSION     DAOQDO010001453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66121_length_1834_cov_2.918494"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..688)
                     /locus_tag="PKZ53_19685"
     CDS             complement(269..688)
                     /locus_tag="PKZ53_19685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ42719.1"
                     /translation="MFKNILVPVGLSERYAGALKVASDLVRQNGGKVTLLHVIELIVG
                     LSLEEGKDLYDRLEQTSHEHLDRLSNQLESAGIPFEEVILYGRVAQEIVRFAEENKSD
                     LIVLASHRIDPCNPGYNWGTNSYKVGILAQCPVMLVK"
     gene            complement(723..>1834)
                     /locus_tag="PKZ53_19690"
     CDS             complement(723..>1834)
                     /locus_tag="PKZ53_19690"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNJ42720.1"
                     /translation="ALKENWPRESLEDLLALPNDADNNLYIEAGSHIASKYKLDHVVA
                     LHEFDVLTAALIREHMNIPGMTASQTRFFRDKYTMRVAAQKAGVRVPDFVQVLNHDDI
                     NAFLHRVPPPWMLKPRSSAGGIGIRKLNGEDQVWNTIKELEHRPLLHERPSHHLMEQY
                     VKGDVYHVDSLISGGQIVFCSASRYSRPPFDIYHHGGIFISCIIEHGSDEENQLFEYN
                     KQILDSMGLTDGVAHVEFIKGAADGEFYFLEIASRVGGAFIAETCEAANGISLWTEWA
                     KLETATPQNPYKPPFAANKYAGIIMSLARQEWPDTSEYTDPEIVHRVKKRYHAGFIVA
                     SPSYQRVQDLLSQYEERISEDFTAVAPPLERPEEL"
BASE COUNT          444 a          475 c          455 g          460 t
ORIGIN      
        1 cggcccaccc actgacgcgg gtggtactga ctcacaggat ttgtgacaga aggaaaagat
       61 ttcctcctgg gaacgccggc accctgccag taactgattg ggtataaacc gcttggtgcc
      121 ggcaagatgc cagcattccc aggaaatatt tatctgaaat gctataccat tggggaaaac
      181 aatgggcctg atccgacttt tacatccagg gtgaaagttg aatcaggccc actgccttaa
      241 aaagcaatcc caaattcttc attgtgcttt acttgaccag catgaccgga cattgcgcca
      301 ggatgccaac cttgtaactg ttggtacccc agttatatcc tggattgcac gggtcaatcc
      361 ggtgtgaggc cagtacaatc aggtcgcttt tgttctcttc agcaaaccgg acaatttcct
      421 gagctacccg gccatagaga atgacctctt caaacggaat tccggcgctt tccaactgat
      481 ttgaaagtcg gtcaaggtgt tcgtgggaag tttgttccag gcgatcatag agatccttcc
      541 cttcttccaa cgacaagccg acgatcaact cgatcacgtg caggagcgtc acctttccgc
      601 cgttttgtct gactaaatcg ctggcgacct tgagggcacc ggcatagcgt tctgaaagtc
      661 cgacggggac gagaatattt ttaaacatat gaccctccgt tccgtccgca gggccacttc
      721 cctcataact cttcgggacg ttcgaggggt ggtgcaactg cggtaaaatc ctcagaaatc
      781 cgctcctcat attgagagag caaatcctgt acccgctgat aactgggtga agccacgata
      841 aaaccggcgt gatagcgttt tttcacccga tggacaatct ctggatcagt atattccgaa
      901 gtatctggcc attcctgacg ggccagcgac ataatgatcc cggcgtattt gttggctgca
      961 aatggtggtt tatagggatt ttgcggggtg gctgtctcaa gcttggccca ttcggtccag
     1021 agactgattc cgttggcggc ttcgcaggtc tcggcaataa aggcccctcc aacccgcgag
     1081 gcgatttcta aaaagtaaaa ctccccatca gccgcgcctt tgataaattc gacgtgagcc
     1141 acaccgtctg tcagccccat gctgtccaaa atctgcttgt tatactcaaa aagctggttt
     1201 tcttcgtcgc tgccgtgctc gatgatacaa ctgatgaaaa ttccgccatg atgataaatg
     1261 tcaaacggtg gacggctata ccgactcgcc gaacaaaaca caatttgccc gcccgaaatc
     1321 aatgaatcaa cgtggtagac atctcctttg acatactgtt ccatcaggtg atgagacgga
     1381 cgttcgtgga gcaacggtcg gtgttcgagt tctttaatgg tattccagac ctgatcttca
     1441 ccgtttaatt tgcggattcc aattccgcca gcgctggatc gaggtttgag catccacggc
     1501 ggcgggaccc gatgcaaaaa agcattgatg tcgtcgtgat tgaggacctg cacaaagtca
     1561 ggcacccgaa cgcccgcttt ctgtgccgca actcgcatgg tgtatttgtc acggaaaaac
     1621 cgggtttgcg aggccgtcat gccggggata ttcatgtgtt cacgaatcaa ggcagcggtc
     1681 aacacgtcaa attcgtgcaa tgccacgaca tggtcaagtt tgtatttgga agcaatatga
     1741 ctgccggctt cgatatagag gttgttgtca gcatcattgg gcaacgcaag caaatcttcc
     1801 aggctttcgc gcggccagtt ttctttgagt gctt
//
LOCUS       DAOQDO010001454         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66194_length_1833_cov_3.007312, whole genome shotgun sequence.
ACCESSION   DAOQDO010001454 DAOQDO010000000
VERSION     DAOQDO010001454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66194_length_1833_cov_3.007312"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /gene="uvrA"
                     /locus_tag="PKZ53_19695"
     CDS             complement(<1..1759)
                     /gene="uvrA"
                     /locus_tag="PKZ53_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ42721.1"
                     /translation="MAIDRITVRGARQHNLKNINLEIPRNTLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVDSLSAYARQFLDQLEHPDVDSIEGLSPAISIDQKTTGRNPRSTVGT
                     VTEIYDYLRLLYSSIGTPHCPECAQPITRQSAEQIVQSILELPVGERVMILAPLVRGR
                     KGEFQKELEKLQKMGFSRARIDGELRFLDEEIRLDKRRNHTIEAVIDRILIKDGIQKR
                     LLESIHTAVSMTDGVVMVAVVDGDEKLYSQKMACPSCGINMPVLEPRSFSFNSSFGAC
                     KTCAGLGVTTEVDPSRLILDPKKTLPKLEFAVTDGHITNQLREGLLALARHHQLGAKL
                     TYADLPPVIKDQFFHGLKEKIVFEYAGYEYRSVWNGVVNLLRERFHDARTDRLRNELE
                     GLILPRTCAECDGKRLRPEALAVRVGGRGIADLTSTAISEAADWFHQLKVSSREEKIA
                     GPLLKEIRSRLSFLNKVGLGYLSLDRPAASLSGGEGQRIRLATQVGSLLRGVLYVLDE
                     PSIGLHPRDTNRLLESLEQLRDLGNTVLVVEHDEETIRRADFVVDLGPGAGSQGGEIV
                     AAGSPDRISADQQSVTGGFL"
BASE COUNT          456 a          518 c          430 g          429 t
ORIGIN      
        1 aaagaaaccc acccgttacc gactgttgat cggctgaaat ccggtcaggc gacccggcgg
       61 ccacaatttc cccgccctgt gaaccagcac ccggccccag atcaaccaca aaatcagccc
      121 gccggattgt ttcttcgtca tgctcgacga ccagcaccgt attccccagg tcccgcagtt
      181 gttctaatga ctcaagcaac cggtttgtat cccgtgggtg tagcccaatg cttggctcat
      241 ccaaaacgta caacacccct cgtaacagag aaccaacttg tgtcgccagg cgaatccgtt
      301 gcccttcacc acctgacaac gaagcggctg gccggtcaag cgataaatag ccaagcccga
      361 ccttattgag gaatgagagc cgtgaccgaa tttccttgag cagtggtccg gctattttct
      421 cttcacgtga ggagactttt aattgatgaa accagtcagc cgcttcagaa atggcggtac
      481 tggtcagatc agcaattccc cggcccccga cccgaacggc cagcgcttct ggacgaagcc
      541 gtttcccgtc acactcagcg catgttcgag gcagaatcaa accttccaat tcattgcgca
      601 accgatcagt tctggcatca tgaaatcgtt cgcgcaggag gttcacgacc ccgttccaga
      661 cgctccgata ctcatacccg gcatactcaa aaacgatctt ctctttgaga ccatgaaaga
      721 actggtcctt gatgactggt ggcaaatcag cataggtcag ttttgcccca agttgatgat
      781 ggcgagccag tgccagcaac ccttcacgca actggttggt gatgtggcca tcggtaaccg
      841 caaactccag tttcggaagt gtttttttgg gatccaaaat caaccgcgac ggatcaactt
      901 cagtggtcac ccccagacca gcacaggttt tacaggcccc aaatgaacta ttgaaggaaa
      961 atgaccgtgg ttctaatact ggcatattaa tgccacaact tggacaggcc attttttgag
     1021 aatacagctt ctcatcgcca tccaccactg ccaccatgac cacgccatcg gtcatggaga
     1081 cggcggtatg aattgattca agcaaccgtt tttggattcc atccttgatc agaatccggt
     1141 caatcacagc ttcaatcgta tggtttcgtc gcttgtccag ccggatttcc tcgtccagga
     1201 accgaagttc cccatcaatc cgggcccggg aaaaccccat cttttggagc ttttccagtt
     1261 ctttttgaaa ttcgcctttg cgtccgcgaa ccagcggcgc caaaatcatc acccgctcgc
     1321 caacgggcaa ctccagaata ctctgaacga tttgctcggc tgactggcgg gtaatgggtt
     1381 gggcacattc cggacaatgg ggagtcccga ttgaggaata gagcaatcgc aggtaatcat
     1441 aaatctccgt cacggtgccc acagtcgaac gggggttacg cccggtcgtt ttttggtcaa
     1501 ttgaaatggc cgggctgagt ccttcaatcg aatcaacatc aggatgttcc agttgatcta
     1561 aaaattgacg tgcgtaagct gaaagtgagt caacatatcg ccgctggcct tccgcataga
     1621 tggtatcaaa ggcgagtgat gatttcccgg agccacttaa tccggtaatc actgtcagtg
     1681 tattgcgcgg gatttcaaga ttgatgtttt tcaggttatg ctgtcgggca ccgcgaaccg
     1741 taatccggtc aattgccatg aatgactcca gtctcaatcg ataggatgaa aaaagacgct
     1801 tattctacct gcgaacccac ccttgagcaa cag
//
LOCUS       DAOQDO010001455         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66195_length_1833_cov_2.989876, whole genome shotgun sequence.
ACCESSION   DAOQDO010001455 DAOQDO010000000
VERSION     DAOQDO010001455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66195_length_1833_cov_2.989876"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKZ53_19700"
     CDS             <1..278
                     /locus_tag="PKZ53_19700"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNJ42722.1"
                     /translation="IRIVTADAKAPMLFSEYDWTQPTLLWLGSEGNGLPNEALQTADT
                     SVRIPMAGQLESLNITVATAILLYEAARQRTSLQAHIPQPPQGETLN"
     gene            293..1102
                     /locus_tag="PKZ53_19705"
     CDS             293..1102
                     /locus_tag="PKZ53_19705"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ42723.1"
                     /translation="MSLNPFSTVLANRTKHNQTVAFTLPEDWLQGRTAFGGVAASLAL
                     AAMREVTAIDRPVRSLQTNFVGPLGPGPFTVSVQLLRQGKSMSQVQATIFQNEQPACV
                     LLAIFGESRPTTLPSFFPEPTLIEPPDAFQDRPYIHGLMPVFTQHIGSRIVAGGVPFS
                     GSSLRHNRIYLKLKDIPVDSELLTVLLSDAIPPAALALADHPIWASTISWWLEFRSVS
                     EARPDDWWLLESEVTALSEGFGHHSYTLWTPSREPIAFGRQMVALFEGRKQ"
     gene            complement(1211..>1833)
                     /locus_tag="PKZ53_19710"
     CDS             complement(1211..>1833)
                     /locus_tag="PKZ53_19710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42724.1"
                     /translation="VPYLVMEYVRGRTLSQWVKEDGPMPISSILHIGAQTARALQEAH
                     SSGIIHRDIKSSNLVVNKKGDVKVLDFGLAKPGLFHLHRPQDSLGDDAELKVIPDITE
                     PGILVGTVTYMSPEQAHGEAEIGPASDIFSLGIVLYEITTGKLPFDGQTYFQTIEGIT
                     KREPKPIQELRPEAPQRLIDVINRALRKAPEDRFHSAAEMAEALEE"
BASE COUNT          408 a          495 c          454 g          476 t
ORIGIN      
        1 gcattcggat tgtgacggct gatgccaaag ccccaatgct gttttctgaa tatgactgga
       61 cgcaaccaac cctgttgtgg cttggttcag aaggcaatgg gctaccaaac gaagcgctgc
      121 aaaccgctga taccagcgtg cggattccaa tggccggaca gcttgaatcg ctcaacatta
      181 ctgtggcaac agcgattctg ctctatgaag cagcacgcca acgaaccagt ctccaggcgc
      241 acatccccca accgcctcaa ggcgaaactc tgaactaaat ttcttttcgg ctatgagttt
      301 gaatcctttt tcaaccgttc ttgcaaaccg aaccaaacat aatcaaaccg ttgcatttac
      361 ccttccagaa gactggctgc agggccgtac tgcgttcgga ggcgttgcgg cctctctggc
      421 cctcgccgct atgcgcgagg tgaccgcgat tgatcgtccg gtgcgttcgc tccaaaccaa
      481 ttttgttggc ccactgggtc cgggaccatt taccgtttca gttcaacttt tgcgccaggg
      541 aaaatccatg tcccaggttc aggcgacaat ctttcaaaat gaacaacccg cctgtgtcct
      601 gcttgcgatc tttggcgaat cacgccccac cacattgccg tcgttttttc cggagccgac
      661 tctgattgaa ccacccgatg cctttcaaga tcggccttat attcacggct tgatgccggt
      721 gttcacccag cacattggtt cgcggattgt ggctggtgga gtcccatttt caggaagttc
      781 actccgacac aaccgtattt atttgaaatt gaaagatata ccggttgatt cggagttatt
      841 gaccgtcctt ctgtctgatg cgattccacc agcagcactg gcgctggcgg accatccgat
      901 ttgggcgagt accatttcgt ggtggcttga atttcggtct gtttctgaag ccagacccga
      961 cgactggtgg ctgctcgaaa gcgaagtgac cgccttgagc gaagggttcg gacaccatag
     1021 ctacacgctg tggacgcctt cacgtgaacc aatcgccttt ggccgtcaaa tggtggcctt
     1081 gtttgaaggc cgaaagcagt gaagaatgaa gaatgaagaa aatacctggt tcttggttct
     1141 tcttcaaaag tattgattcc taagaaccaa gcaccaagca ccagatactc aattcttcat
     1201 tctcaactct tcattcttcc aatgcttcag ccatttcggc ggctgagtgg aaccgatcct
     1261 caggagcttt gcgcagggcc cggttgataa cgtcaattaa acgctgaggt gcttcgggtc
     1321 tgagttcctg aattggtttg ggttcgcgtt tggtaatgcc ttcaatggtt tggaagtagg
     1381 tctgtccatc aaatggcagt ttcccggtgg tgatttcgta gagcacaatt cccagcgaaa
     1441 aaatatcgct tgccggcccg atttcggctt caccgtgggc ctgttccggt gacatatagg
     1501 ttactgtccc gacgagaatt cctggctccg taatgtctgg aatcactttg agttcggcgt
     1561 catctcccaa agaatcttgt ggccgatgca ggtgaaataa ccctggtttg gccagtccga
     1621 aatccaggac tttgacatcg ccctttttat tgaccaccag attggatgac ttgatatccc
     1681 gatggataat cccgctggaa tgggcctcct gcagcgcccg tgcggtttgg gcgccgatgt
     1741 gcagaattga agaaattggc atcggaccgt cttctttcac ccactgactg agcgtccgcc
     1801 cgcgcacata ctccatcacc agatagggca ctt
//
LOCUS       DAOQDO010001456         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66196_length_1833_cov_2.917885, whole genome shotgun sequence.
ACCESSION   DAOQDO010001456 DAOQDO010000000
VERSION     DAOQDO010001456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66196_length_1833_cov_2.917885"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKZ53_19715"
     CDS             complement(<1..674)
                     /locus_tag="PKZ53_19715"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HNJ42725.1"
                     /translation="MRFPSRSESFDRIREKVISGQAALEFGDSAQALTLLDEAVGQVL
                     ALDWSDEAVWKRWPVMQGVLQFAKQYLAQEIAESSLAPDVSDNSDEPRFLTSIPELMI
                     VNREMRAIIDQIVRIRHTRMNVLITGESGVGKELLAKAIHLESDLRDNVYIPFNCAAV
                     APELLESRLFGHRRGAFTGAVSDQRGVIRAAEGGTLFLDEVGELPLEVQPKLLRFLQE
                     GEIQPLG"
     gene            829..>1833
                     /locus_tag="PKZ53_19720"
     CDS             829..>1833
                     /locus_tag="PKZ53_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42726.1"
                     /translation="MRNQLRRLACLILVFFSGMTGMVPPLSAQTARFSIASQHAQIQE
                     AMARRTYQVAIRLLDELKKAEPELYQLNNYEYLRARLADWTGDGATAIQLYRTVAEQK
                     SKLSPFAMRHLAELQHERKDFKNEQVYWQLLRTSYPNSPAAKGTLGKLAESYEHSGNY
                     RAAASVYQNISTTDREILGKTALALRKADKADAARLIFQRLLQTGKDDQALLAAENLD
                     ELGGETLTEADHLTRGRLFLKNRHAEGAIRHFQTIVRHYPACKQMPEVLFGIGRAHYI
                     SERWDEAITWYDRAHREFPKTPEGELAFYQLGHAHQNAGRTREAVAQYEAFIAEYPKS
                     E"
BASE COUNT          496 a          510 c          436 g          391 t
ORIGIN      
        1 cctaaaggct ggatttcacc ttcctggagg aaccgaagca atttcggctg aacctcaagc
       61 gggagttccc caacttcatc gaggaacaaa gttccgcctt cagccgctcg aatcacacca
      121 cgctggtcag agaccgcacc ggtaaaggcc ccccggcgat ggccaaagag acgactttca
      181 aggagttcag gggcaacggc agcacaattg aagggaatgt agacgttatc gcgcagatca
      241 ctttcaaggt gaattgcctt ggccagtagc tctttgccaa caccagattc acccgtgatg
      301 agcacattca tccgggtgtg ccggatgcgt acaatttgat caatgatggc ccgcatttca
      361 cgattgacaa tcatcaattc aggaattgat gtcagaaagc gtggttcatc tgaattgtct
      421 gaaacatctg gggcaagtga actttccgca atttcctggg caagatattg tttggcaaac
      481 tgtaaaacac cttgcatcac gggccatcgc ttccagacgg cttcgtcaga ccaatcaagc
      541 gctaacacct ggcccacagc ttcgtcgagc aacgtcaggg cctgggctga atctccaaat
      601 tcaagggccg cctggccaga aatcaccttt tcacgaatgc ggtcaaatga ctcggaacgt
      661 gaaggaaaac gcataaattt taagggtaga caacagactt ttttgaggtt tgaaatccat
      721 gaagggagcg cataaacata ccacacccaa tgaaacgtgt gcgatacttt tcccaatcac
      781 tccctggtcc acctctttgc caacacaatc aaaaacaacg gtgacattat gcgcaatcaa
      841 ctgcggcgac ttgcctgcct gatccttgtc tttttttcag gaatgaccgg aatggttcct
      901 ccactttcag cccaaacagc caggttttcc attgcctccc agcacgctca aatccaggaa
      961 gccatggcgc gtcgcactta ccaggtcgcc atccgtctgc tggatgaatt gaaaaaagct
     1021 gagccggaac tatatcagct caacaattat gagtatttgc gcgcccgact ggctgactgg
     1081 actggtgatg gggccacagc catccagctt tatcgcactg tggccgagca aaagagcaaa
     1141 ctctccccgt ttgccatgcg gcatctggct gaacttcagc acgaacgcaa agacttcaaa
     1201 aacgaacagg tgtactggca attgttgcgg acatcctatc caaacagccc ggcggcaaaa
     1261 ggtacactgg ggaaactggc tgaaagttat gaacacagtg gcaactaccg cgctgccgcc
     1321 agtgtgtacc agaatatttc aaccacggac cgcgaaattt tgggaaaaac ggcgctggca
     1381 cttcgcaaag cagataaagc cgacgcggcc cgattgattt ttcaacgcct gcttcaaacc
     1441 gggaaagatg atcaggcatt gctggcggct gaaaaccttg acgaactcgg cggcgaaacc
     1501 ctcactgaag ccgaccacct cactcgcgga cggctgtttc tcaaaaatcg ccatgccgaa
     1561 ggtgccattc gccattttca aaccatcgtc cgacactatc cagcctgcaa acaaatgccc
     1621 gaagttctgt ttggaatcgg acgcgcccac tacatttcag aacgctggga tgaagctatt
     1681 acctggtatg accgcgcgca tcgtgaattt cccaaaaccc cagagggtga actggctttt
     1741 taccagcttg ggcacgccca tcaaaacgct ggccgaacgc gtgaagccgt tgcccagtat
     1801 gaagccttca tcgcagagta cccaaagagt gaa
//
LOCUS       DAOQDO010001457         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66275_length_1832_cov_2.765898, whole genome shotgun sequence.
ACCESSION   DAOQDO010001457 DAOQDO010000000
VERSION     DAOQDO010001457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66275_length_1832_cov_2.765898"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1268)
                     /locus_tag="PKZ53_19725"
     CDS             complement(342..1268)
                     /locus_tag="PKZ53_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ42727.1"
                     /translation="MKETVRLYIQSKSSSREVAATMKIDQSTVIRWTQQLGEKCADTI
                     QTNRQLRPQWRGWLGIDGKFIRVGGHHMACLLAIDLATLDIPHCDLVTAEDEAGCRRF
                     LEIVRQQVGAGRGIVSDLGKGKVWLKLIAELFPDVPHQACIVHFDRYVQQTLPKSKNN
                     RHAAEHELLRQLIKRLLYAQTFADAEEVFVRLLDCQSYFQVACQRTILTSLKRHFDLL
                     TAHFHTPGLDRTNNVTENLIKQLDRKIFLLNDFSTPQAALNFLKLWVLNYRFKPFSAS
                     SIETRNGRSPLALAGVETNHWDWLDFGISPNN"
     gene            complement(1294..1455)
                     /locus_tag="PKZ53_19730"
     CDS             complement(1294..1455)
                     /locus_tag="PKZ53_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42728.1"
                     /translation="MTKKTVSLLPKPAGSKTRLDPLPAEDQTTSRTTKKATLPVSNLW
                     AHLFPRTLE"
     gene            complement(1578..>1832)
                     /locus_tag="PKZ53_19735"
     CDS             complement(1578..>1832)
                     /locus_tag="PKZ53_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42729.1"
                     /translation="NKAPGWPMSLGDNKKAIECLTKALKFGPKFYLNHLYLAEAYLSL
                     RDKKKAKEHLDWLLAAPLSPHHEREDRVTQKEAQALLKKL"
BASE COUNT          408 a          437 c          482 g          505 t
ORIGIN      
        1 tgggaattgt ggttcgtgtt ccgaattatg actgaagacc tgccaagtgc caaatcagga
       61 ggaaaaaatt tccattgaag cctaccgcct gcgacgggta ttctgaagtt tgttagaaca
      121 atacttttcc ttccccaacg gcgcgcatca ccggctgacg aagcggcaac caactttccc
      181 gtcattgatg ctgcccgctg atgtcagtcc gcgtgcatgc gctggttggg ctggtttgat
      241 gacttgccac tatgaaatct aacccaaccc atgacacttg ccaaccgggt ggtttggatt
      301 tggttttgga ttttggattt tggattttgg gcactgatgc attaattgtt gggactgatc
      361 ccaaaatcca accaatccca gtgattggtt tcaacaccag ccagcgcgag cggcgaccgc
      421 ccattgcggg tttcgatgga actggctgaa aagggtttaa agcgatagtt gagcacccac
      481 agtttgagaa aattgagggc cgcttgtgga gtggaaaaat cattgagcag gaagattttg
      541 cggtccaact gcttgatcag gttttcggtc acattattgg tccgatccaa gcctggcgta
      601 tgaaaatgag cggtcaacaa atcaaaatgg cgcttcaatg aagtcagaat ggttctttga
      661 caagccactt gaaagtagga ttgacagtcg agcaggcgga caaacacctc ttcggcatcg
      721 gcaaaagtct gggcatacag cagccgttta atgagttggc gcaaaagttc gtgttcagcg
      781 gcgtgtcggt tgtttttgga ttttggcaag gtctgttgga catagcggtc aaaatggaca
      841 atacaggctt ggtgcggcac gtcgggaaac aactcggcga tgagtttgag ccacactttc
      901 cccttgccca gatcactgac aatcccgcgc ccggctccga cttgctggcg cacaatttcc
      961 agaaatcggc ggcaaccggc ttcatcttcg gcggtcacca gatcgcagtg cggaatgtcc
     1021 aaagtcgcca aatcgatggc cagcaaacag gccatgtggt ggccgccaac ccggatgaat
     1081 ttgccatcaa tccccagcca accgcgccac tgcgggcgca gttgccgatt ggtttgaatg
     1141 gtatcggcac acttttcacc aagttgctgg gtccagcgaa tgacggttga ttgatcgatt
     1201 ttcatggtgg cggccacttc ccgactcgat gatttacttt ggatatagag tcggaccgtc
     1261 tctttgataa agctctcgct atgtcgttta tgcttactcc agcgttcttg ggaaaaggtg
     1321 cgcccacagg tttgacaccg gtagcgttgc ttttttcgtg gttcgggatg tcgtttggtc
     1381 ttcggctggg agtggatcca gccgtgtttt tgaacccgct ggctttggca aaagggacac
     1441 cgtttttttt gtcattttag cctccaattc ttcgtgcatc cggattgttg atggctactc
     1501 tttctttttc aatggcttac agcattttag ccaacaatta atgcatcagt gcccaattct
     1561 caattctcaa ttctcaatta gagttttttg agcagcgcct gagcttcctt ttgcgtcact
     1621 ctgtcttcac gttcatggtg aggcgagagt ggagccgcca gcagccaatc caaatgctct
     1681 ttggcttttt tcttgtcccg gagggacagg taggcttcgg caagatagag gtggttcagg
     1741 taaaatttgg gaccgaattt gagggctttg gttaggcatt caatcgcttt tttattgtca
     1801 cccaatgaca tcggccagcc cggagccttg tt
//
LOCUS       DAOQDO010001458         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66286_length_1832_cov_2.451322, whole genome shotgun sequence.
ACCESSION   DAOQDO010001458 DAOQDO010000000
VERSION     DAOQDO010001458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66286_length_1832_cov_2.451322"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="PKZ53_19740"
     CDS             complement(<1..1620)
                     /locus_tag="PKZ53_19740"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42730.1"
                     /translation="MILLVLLGISTTFGQQVTPPVAPLTSPGSNQSNQPTPLSFGIPL
                     RQTVVTGEKYVYQLSLPENSILEGKVDQHGVTATIRLRSADQSLRLERSFPLGTYGSQ
                     MIFFVSGQGGEYQLEIEPTESQSASSFFELTVSERHPATPDDFCRFADYVNLPQFFDE
                     VQKFSQTDDPEQLHRALAKLEAGIQLSLRLGDKRAEGLFVQESGYVYKRLNDTQKALE
                     KHQQAVQLFHDAGDADTECVALSALGGVYQYSGRLPEALKTYQQMLELSQKLGLRFNE
                     ATCLGNIGATHKLAGEQQTAIEYFEKALVIHREVKNQTDEIVTLDDIGLAYLQMGNPQ
                     KAIEFVTQAYKLAESLHIDGETTRTLNDLAGIYDMLGEYETALGYYSKALEIARKNKF
                     THSEANLANNLGYIYLMTKDYTKGSEILKQAIEISHRYGYQYIEASSLTTLGVIYRRM
                     GDLPQALSHYLQALELHKKISTPIIQASTFNNIGRLYHLTKEYPKAAEAIQTALRLGQ
                     QTGDIETQIRALGNLGPLQKDQHQFLEAKATL"
BASE COUNT          451 a          413 c          457 g          511 t
ORIGIN      
        1 aagggtggct ttggcttcta aaaattgatg ctgatctttt tgtaatggcc ccagattccc
       61 aagtgctctg atttgggtct cgatatcacc agtttgctga cccagccgaa gggctgtttg
      121 gatggcctcg gcggctttgg ggtactcttt ggtcaggtga tagagccgtc cgatattatt
      181 gaaggttgat gcttgaatga tgggggtgct gattttttta tgcaattcga gcgcctgaag
      241 gtaatggctc aacgcctggg gcaaatcacc cattcggcgg tagatgacac caagcgtggt
      301 cagcgaggag gcttcaatat actgatatcc atagcgatgg ctgatttcaa ttgcctgctt
      361 gaggatttct gacccttttg tatagtcttt ggtcatcaga taaatatacc caagattatt
      421 cgcgagattg gcttcactgt gggtaaattt attttttcga gcaatttcca gagctttgga
      481 ataatagcca agcgctgttt catactcacc caacatgtca tagatgccag ctaaatcatt
      541 gagcgtcctt gttgtttccc catcaatgtg caatgactca gccagtttgt atgcctgagt
      601 cacaaattca attgcctttt gaggattccc catctgcaaa taggccaggc ctatatcatc
      661 aagagtcact atctcatcag tctggttttt gacttcacgg tggatgacca gcgctttctc
      721 aaaatattca atcgctgttt gttgctcacc cgccagtttg tgagtggcac ctatattgcc
      781 caaacacgtt gcttcattaa atcggaggcc cagtttctga ctaagttcca gcatctgctg
      841 ataggttttc agggcttcgg gaagccgtcc tgaatattga tagacccctc ccaacgcgga
      901 aagtgcgaca cattcagtgt ctgcatcccc ggcatcatga aaaagctgga ctgcctgttg
      961 atgtttttcc agggctttct gagtgtcgtt caaacgcttg tatacgtatc ccgattcctg
     1021 cacaaacagt ccttcagcgc gcttatcacc aaggcgcagg ctcaattgaa ttccagcctc
     1081 aagtttggcg agtgcccggt gtagttgttc tgggtcatcg gtttgggaaa atttttgaac
     1141 ctcatcaaaa aattgcggca gattcacata atcggcaaag cgacaaaaat catctggcgt
     1201 ggctgggtgc cgttcactga ccgtaagttc aaaaaagctg gaagcggatt gtgattcggt
     1261 tggttcaatt tcaagctggt actcgccacc ctgtcctgaa acaaaaaaga tcatctgcga
     1321 tccataggtg cccaaaggga atgaccgttc aagtcgcagg ctctggtcag cacttctgag
     1381 tcgaatggtg gcggtaaccc catgttgatc aacttttcct tcaaggattg aattttcagg
     1441 aagtgagagc tgatagacat atttctcgcc tgtcacgact gtttggcgaa gcggtatccc
     1501 aaaagagagc ggtgtgggtt ggtttgattg atttgagccg ggtgaggtga gcggggcaac
     1561 cggcggagtc acttgctggc caaacgtggt ggaaatcccg agcaatacaa gcaggatcat
     1621 gccttgcacg aaaacaaggc tctttgggca tcggttcatc gcggacataa ctgggtaacc
     1681 tgtgtctaag gggatatctg ggatctaact gacattgctc acaccatagt atacaaggac
     1741 agatttttga acaaaaaaga cgaaccgtgg ccatcacagc cacggttcgt cttgtgggaa
     1801 aaacctgatg atgttgaaga cttgctaaac ag
//
LOCUS       DAOQDO010001459         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66393_length_1830_cov_3.560563, whole genome shotgun sequence.
ACCESSION   DAOQDO010001459 DAOQDO010000000
VERSION     DAOQDO010001459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66393_length_1830_cov_3.560563"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKZ53_19745"
     CDS             <1..431
                     /locus_tag="PKZ53_19745"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNJ42731.1"
                     /translation="FFILHSQFFILHSQFFILNSSLMSNPRSPWPTLKDWWEALRWVL
                     LVFTVVVALTLAGSFVSYETVLSSGHPWLPRTHCSGCLFCGMTRSFCALSSGHWAEAV
                     RWNRGGPVLYSAGWLWLAGSLVVFSRLVRTRSRTQVVTDS"
     gene            450..1811
                     /gene="larC"
                     /locus_tag="PKZ53_19750"
     CDS             450..1811
                     /gene="larC"
                     /locus_tag="PKZ53_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNJ42732.1"
                     /translation="MKTLYFDCFAGTSGDMTIGALLHLGVDFEVLKQELSKLDLGDYE
                     LSVQSVSRAQISGIKFDVTINGRLEQPAPLDTSHHHHSHDHSHHEHLPEPASHIQATH
                     GQPQAETHAHRTVTDIHDLIDKAGLSSWVTRQAKAIFWRLAQAEGTIHGVPPAQVHFH
                     EVGAVDAIIDIVGACIGFEFLGIERFIASPLQVGFGFVKCAHGRYPIPAPGTAELLKG
                     VPIYSLDIEGEFVTPTGAAIVTTLCSEYSRIPNFTPEKIGYGAGGRDFPRFPNLLRLM
                     VGQTEESNSAYLMTQPTLCDTTSSITVLEANIDDMNPQVFGHVMELLLNQGALDVFYT
                     PVQMKKNRPGTLLTVLCEDHLAKALMALVLRETTTLGVRSYRADRQVLDRQILTVETE
                     FGPTRIKVATLNGDVLKAMPEYDDCHQQALQAQVPLTVVQSAALTAFFGIKNEELRMK
                     NEE"
BASE COUNT          415 a          458 c          467 g          490 t
ORIGIN      
        1 ctttcttcat tcttcattct caattcttca ttcttcattc tcaattcttc attctcaatt
       61 cttcccttat gtccaaccca agaagcccct ggccaacact aaaagactgg tgggaagccc
      121 tgcgatgggt gcttctggtg tttaccgtgg tggtggccct gacgctggca gggtcgtttg
      181 tatcttatga aaccgtactg agcagcgggc atccctggtt gccacggacc cactgcagtg
      241 gttgtctttt ctgtggtatg acccgtagct tttgtgcgct ctcaagtgga cattgggccg
      301 aggctgtacg gtggaatcgt ggcggaccag ttctctattc agccggctgg ttgtggctgg
      361 cgggaagtct ggttgttttc agccggctgg ttcgcaccag gtcgagaaca caggttgtca
      421 ctgactcata attcatcact cataactcca tgaagacact ttattttgat tgttttgctg
      481 gcaccagcgg agatatgacc atcggggcgc tcctgcatct cggggttgat tttgaggttc
      541 tgaaacagga actaagcaag ctggatcttg gcgactatga gttatcagtt caatcggttt
      601 cgcgagccca aatttccgga atcaaatttg atgtcacaat caatggccgc cttgaacagc
      661 cagcgccgct tgataccagt catcatcacc attcccacga tcattcacat catgagcatt
      721 tgcctgagcc tgccagccat atccaggcga ctcatggtca gcctcaggct gagacgcatg
      781 cgcatcgaac cgtcaccgat attcatgacc tgattgacaa ggctggactt tcctcctggg
      841 tgacccggca ggccaaagcc attttctggc gactggccca ggctgaaggc acaattcatg
      901 gtgtcccgcc ggctcaggtg cattttcatg aagttggcgc ggttgatgct attattgata
      961 ttgttggcgc ctgtattggc tttgaattcc ttggtattga acgctttatt gcgtccccac
     1021 tccaggttgg cttcggattt gtcaaatgtg ctcacggacg ctatccgatt ccggcgccag
     1081 gaaccgctga actgctcaaa ggcgtgccga tttattcact cgatatcgaa ggtgaatttg
     1141 tcacgccaac cggcgccgcg attgtgacaa cgctgtgctc tgagtactca cgcatcccaa
     1201 atttcacacc tgaaaaaatt ggatatggcg ctggtggccg tgacttccca cggtttccca
     1261 atctgttacg gctgatggtt ggccaaacgg aggaatcaaa ctcagcttat ctgatgaccc
     1321 aacccacgct ttgcgatacc acgtcatcaa tcacggtgct cgaagccaac attgacgata
     1381 tgaatccgca agtttttggt catgtgatgg agttgctgct caatcaaggg gcacttgacg
     1441 ttttttacac tccggttcaa atgaaaaaaa accgccctgg cacgttgtta actgttctgt
     1501 gcgaagatca tctggcaaag gcattgatgg ctctggtgct gcgtgagacg accacgcttg
     1561 gcgttcgttc gtatcgggct gaccggcaag ttttggatcg ccagatactc acggttgaga
     1621 ctgaatttgg accgactcgg atcaaagttg cgaccctgaa tggagacgtg ttgaaagcca
     1681 tgccggaata tgacgattgc caccaacagg cgttgcaggc gcaggttcca ttgacggttg
     1741 tccaatcagc cgcgctgaca gccttttttg gaataaagaa tgaagaattg agaatgaaga
     1801 atgaagaatg aagaatgaag aatgaagaat
//
LOCUS       DAOQDO010001460         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66554_length_1828_cov_3.236887, whole genome shotgun sequence.
ACCESSION   DAOQDO010001460 DAOQDO010000000
VERSION     DAOQDO010001460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66554_length_1828_cov_3.236887"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKZ53_19755"
     CDS             <1..197
                     /locus_tag="PKZ53_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42733.1"
                     /translation="SGRPTKSQLDYAGVLEKEFTKAGQDFEALVTTEVNAINSKLQVK
                     KLDPLKVQSREEYDKAQSGR"
     gene            203..1429
                     /locus_tag="PKZ53_19760"
     CDS             203..1429
                     /locus_tag="PKZ53_19760"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNJ42734.1"
                     /translation="MISARTSLKVFVVCLAMTGIALTASMRTQALPDYLKIFASDPLA
                     RPEWKITCATCHESAKGGGVRNAFGKAFQAAGHKITPELRQQFPDRFLQAPEPPKVAF
                     SDGQSQVVIEMNGRKWRIDPTTKTVTDEGPVETAPPPAVAEVKTPVTPDGIHRPGDDK
                     IISLPTGRAVPKNGLIVNFSHRFSLNEDRTFGGFFGLDGFSNSSFGFLYGITNRIHVG
                     FNRSPSFIGRPIEFFGGFQISDEMKGDPITSQVRVGVEASNNFTRNYVTSISGTVARS
                     LTRHAQIAVTPTISFNNRPLLAITPVPELPGKTTFALGAGLTVKFRPSLNFIIEANQR
                     LEGKLGTPRPAFGFGIQKVTGDGRHAFALTFNNSPGFTTAQRSGTRATLFGEGTMLDD
                     TFSGLSIGFNITRRMF"
     gene            1612..1821
                     /locus_tag="PKZ53_19765"
     CDS             1612..1821
                     /locus_tag="PKZ53_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42735.1"
                     /translation="MPLDVASSAFTPEQARIALDEAVRLFLETATECGTLEDVLEDAG
                     YTLQDGQWISPVWVSIERHSLLVGV"
BASE COUNT          450 a          487 c          466 g          425 t
ORIGIN      
        1 atagtggacg tccaaccaaa tcacagcttg attatgcagg cgtgctggaa aaggaattta
       61 ccaaagctgg tcaggacttt gaggcgcttg tgacgacgga ggtaaatgca ataaactcaa
      121 aactccaagt caaaaaactc gatcccctca aggttcagtc ccgcgaagaa tacgataaag
      181 ctcaatcagg aaggtagatt ccatgatttc tgcacgaacc tccttgaagg tattcgttgt
      241 gtgtctggca atgaccggta tcgcattgac cgcctcgatg cgcacccagg cattgccaga
      301 ctatcttaaa atttttgctt ctgatccact ggccagacct gaatggaaga tcacgtgtgc
      361 cacctgccat gaaagcgcca aaggcggcgg cgtccgcaac gcttttggca aagcgtttca
      421 ggcggctggg cacaaaatca caccggaact gcgccagcaa tttccagatc gttttttgca
      481 agctcctgaa ccgccaaagg tggccttttc cgatggccag tcccaggtag tcattgaaat
      541 gaatggccga aagtggcgga ttgacccaac caccaaaacc gtgacggatg aaggtccagt
      601 tgaaaccgcc ccgccgccag cggtggctga ggtcaaaacg ccagttaccc ctgacggtat
      661 tcaccgtccc ggagatgaca aaatcatcag tcttcccaca ggacgggcgg ttcccaaaaa
      721 cggactgatc gtcaatttca gccatcggtt ttcgctcaat gaagaccgca cctttggtgg
      781 attttttggc ctcgacggct tttcaaactc atcgtttggg tttttgtatg gcatcaccaa
      841 tcgaattcac gttgggttta accggtcgcc atccttcatt ggccgcccga ttgaattttt
      901 cggtggattc cagatcagtg atgaaatgaa aggcgacccg attacctcgc aggttcgggt
      961 tggagtcgag gccagcaaca actttacccg caactatgtg acgagtattt ccgggacggt
     1021 ggcccgcagc ctcacccgcc acgcccaaat tgccgtcacg cccaccattt cattcaacaa
     1081 tcggccactg ctggccatta ccccggtgcc ggaactcccc ggcaagacta cctttgccct
     1141 gggcgccgga ttgacggtga aattcaggcc aagtttgaac ttcatcatcg aagccaacca
     1201 gcgacttgaa ggcaaactcg gtaccccacg tccggcgttt ggatttggga ttcagaaagt
     1261 cacgggtgat ggtcgtcacg cctttgcgct gacctttaac aacagccctg gctttaccac
     1321 ggcgcaacgt tccggcacac gggcgacgct ctttggtgaa ggcaccatgc ttgacgatac
     1381 cttcagcggt ctgtcaattg ggtttaacat cacccgccgg atgttttgat tgagggataa
     1441 aattcagggc tgaggaaaac gggctgaaga ttttcaaccc aggctcgaat tcttcagccc
     1501 caagccgtca atccccaggc ccaagttctt gaaatgagtg cagaaaaagc tatgaaaatc
     1561 gagtacacgg ttcacatttg gcaggaaggt taccaattta tcgctcacgc tttgccgctc
     1621 gatgttgcaa gttcggcttt cactccagaa caggcgcgaa ttgcacttga tgaggccgtt
     1681 cgattgtttc ttgaaacagc aactgagtgt ggaactctcg aggacgtgct tgaagatgca
     1741 gggtacacac tccaggatgg gcagtggatc agtccggtgt gggtatccat cgagcgacat
     1801 tcattactgg ttggagtgta agtgcgat
//
LOCUS       DAOQDO010001461         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66558_length_1828_cov_3.119571, whole genome shotgun sequence.
ACCESSION   DAOQDO010001461 DAOQDO010000000
VERSION     DAOQDO010001461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66558_length_1828_cov_3.119571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="PKZ53_19770"
     CDS             complement(<1..1712)
                     /locus_tag="PKZ53_19770"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42736.1"
                     /translation="MTSTTAPDRTQKAIFWGIGLISLASLLFELTLTRIFSVVMYYHF
                     AFMVISLAMFGFGVSGLMTYIKPDWFPREKLGTKLWRYSIYFSVWTVVSLLCVLQMQV
                     DLSFRPENIQKLVLIYILASVPFFFVGLCVSIAMTHLSEVIGKLYFYDLLGASLGCMI
                     AIPVMNFFGGPGAVLAATALAALGGLFFARVAQEEVNPKAQLSPSERAPGLILLGGLM
                     ALAGLALMIPGLKARGMAYLSSDQLSSVAVFASVIGTILCLSGFFSRRQRHIWLPVSL
                     AVSVLILAGANTQVEFLRVIFSKGNREENIVFSKWNSFSRISVEIYPPTGAHTMRIDA
                     DAATAVVQTGGKITVRKPFEEGVSGVAYHIRRGAGQVLIIGPGGGWDIATAVAHDAKN
                     ITGCEINPIIVNDVMKGQFKQFTGGLYEFPNVKVQVGEGRSFVRNSGQQFDIIQATLV
                     DTWAATAGGALSLTENNLYTVEAFEDYFKHLTDSGMITMSRWLMKPDQQNLRLVSLGI
                     EALRRLGVTQPERHFMLVSSGRGFEDTNQQICNVMMKRTPFTDEEVRAMEQEVQKYGF
                     SVVYT"
BASE COUNT          510 a          506 c          421 g          391 t
ORIGIN      
        1 gtatacacca cactgaaccc atatttctgg acttcctgct ccatcgcccg cacttcttca
       61 tcggtaaacg gcgttcgctt catcatcacg ttacagattt gttgattggt atcttcaaat
      121 ccgcgtccag aactcaccag cataaagtgc cgctcaggct gggtcacccc cagccgacgc
      181 agagcttcaa tgcccagtga aaccagccgt aaattctgct ggtcaggttt catcaaccag
      241 cgcgacatgg tgatcatgcc ggagtcagtt aaatgcttga aataatcttc aaaggcttcg
      301 accgtgtaga gattgttttc cgtcaatgac agtgcgccac cagccgttgc cgcccaggta
      361 tcaaccagtg tggcctgaat aatgtcgaat tgttgcccag agtttcgaac aaacgaccgg
      421 ccttcaccca cctgaacctt tacatttggg aattcataca gtccaccggt gaactgctta
      481 aactgtccct tcataacatc gttgacgata atcgggttga tttcgcagcc ggtgatattt
      541 tttgcatcgt gagccacagc ggtggcaatg tcccatcctc ccccaggtcc aataatcaaa
      601 acctgacccg caccgcgtcg gatatggtag gcaaccccag agacaccttc ttcaaacggt
      661 ttgcgaaccg tgattttccc gccagtttgg acgacggcgg tggcggcgtc ggcatcaatc
      721 cgcatcgtat gcgccccagt cggaggataa atttccacgg aaatgcggga aaacgagttc
      781 cattttgaaa acacaatgtt ctcttcccga tttcctttgg aaaaaatcac ccgcaaaaac
      841 tcaacctggg tattggcacc cgctaaaatc aacaccgaaa ccgccagtga aactggcagc
      901 cagatgtgtc gttgacggcg cgagaaaaag ccactcaggc agagaatggt tccaatcacc
      961 gaagcaaaaa ccgccacact gctcaactgg tcactcgata aataggccat tcccctggct
     1021 ttgagccctg gaatcatcag cgccaaacca gccagcgcca tcaatccacc cagcaagatc
     1081 agccctggcg cccgctctga aggtgaaagc tgtgctttgg gattgacttc ttcctgggcc
     1141 actcgtgcaa aaaacagacc accgagtgcg gccagcgcag tggcagccag aacggcaccc
     1201 ggaccaccga aaaagttcat caccggaatg gcaatcatac agcctaaact ggcgccgaga
     1261 aggtcataaa agtagagttt cccgatcacc tcgctcaaat gagtcatggc gattgagaca
     1321 cataacccga caaaaaagaa tggcacggat gccagaatat aaatcaacac cagtttctgg
     1381 atattttctg gtcgaaaaga gagatcaacc tgcatctgga gcacacacag caaactcacc
     1441 acggtccaca cactgaaata aatgctgtag cgccagagct ttgttcccag cttctcacgc
     1501 ggaaaccagt caggtttgat ataagtcatc aggccgctga caccaaaccc gaacatggcc
     1561 agtgaaatca ccatgaaggc aaaatggtag tacatcacga ccgaaaaaat tcgggtgagc
     1621 gttaattcaa acaacagtga agcgagcgaa atgaggccaa ttccccaaaa aatagctttc
     1681 tgtgtccgat ccggagcagt cgtcgaggtc attgtctaga gttgtccttg tctttgatta
     1741 caggtcaaga atgggttgct tgagataagg ggcgggattg tacggggagt tgcccgacat
     1801 cacaagggcc aaccaggaaa gaccatct
//
LOCUS       DAOQDO010001462         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66633_length_1827_cov_3.245485, whole genome shotgun sequence.
ACCESSION   DAOQDO010001462 DAOQDO010000000
VERSION     DAOQDO010001462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66633_length_1827_cov_3.245485"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKZ53_19775"
     CDS             complement(<1..334)
                     /locus_tag="PKZ53_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42737.1"
                     /translation="MNINHQVLSRRVWQFRTGCTVLILICSLFFSPLFRPGPVSAQTA
                     TPLSPKETVRRFYELLRQRKYVEAFQYSVYASAVETLSPAQFKLLESDFQAMADAIPE
                     KVMLNGEQI"
     gene            complement(442..1755)
                     /gene="asnS"
                     /locus_tag="PKZ53_19780"
     CDS             complement(442..1755)
                     /gene="asnS"
                     /locus_tag="PKZ53_19780"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNJ42738.1"
                     /translation="MKQTYIADIAQHLGQEVTLRGWLYNSRSSGKLQFLEVRDGTGLI
                     QTIVFKKAVTETVWETAEKLTQESSIIVTGVPREHPKRPGVYELDVQNIELVQLTENY
                     PITPKEHGVEFLMDHRHLWLRSRRQHAILRVRHEIVKAVRDFFDSNGFILADTPILTP
                     AACEGTTTLFEVDYFDDNKAYLTQSGQLYNEATAAAFGKTYCFGPTFRAEKSKTRRHL
                     TEFWMVEPEVAYADLNDIMDLAEEFLCFVVGRVLANRRPELEALERDISKLETLTRPF
                     PRLHYDDAVKMLHQGFDEGKLPTRFEWGGDFGAPDETYISGHFDCPVFVHRYPAKIKA
                     FYMEEDPERSELSLSVDVLAPEGYGEIIGGGQRMQSLERLCERIAEHELPQEAFDWYL
                     DLRKYGSVPHAGFGMGIERCVTWLCGIEHVRETIPFPRMLYRLKP"
BASE COUNT          468 a          446 c          457 g          456 t
ORIGIN      
        1 tgatttgttc tccgttgagc ataactttct caggaatggc atccgccatt gcctgaaaat
       61 cgctttctag cagtttgaat tgagctggtg aaagagtttc aaccgctgag gcatagacac
      121 tgtattgaaa ggcttcaaca tattttcttt gtctgagcaa ctcataaaac cggcgtacgg
      181 tttctttggg tgaaagaggg gttgcggtct gggctgatac aggtccgggc cgaaacagtg
      241 gggaaaagaa aagactgcat atcaaaatca aaaccgtaca cccggtacgg aattgccaaa
      301 cccgccttga tagaacctgg tggtttatgt tcatagccaa cctctttcaa cgagtaaaga
      361 tttgggattg gagggattga aagaagcgac ctctctcagc cccctttccc tacagataca
      421 gtgtcgtagt ctctgtgccg ctcagggttt gagccgatac agcatgcgtg gaaacggaat
      481 tgtctcacgg acatgttcaa tgccacacaa ccaggtgaca cagcgctcga tccccattcc
      541 aaagccagcg tgcgggacag aaccatactt gcgcaaatcg agataccaat caaatgcctc
      601 ctgggggagt tcgtgctcag cgatgcgttc gcacagacgt tcaagtgact gcatgcgctg
      661 accgccacca ataatttcgc catagccttc tggcgccaga acatcaacgg ataaagacag
      721 ctctgaccgt tctggatctt cctccatata aaaggctttg atcttggctg gataccgatg
      781 gacaaacacc gggcaatcaa aatgtccaga gatataggtt tcatccggtg ccccaaaatc
      841 cccaccccat tcaaaccggg tcggaagctt gccttcgtca aacccctgat gcagcatttt
      901 gacggcgtca tcataatgga gtcgaggaaa tggtcgcgtg agggtttcca gttttgaaat
      961 atcacgctcc agcgcttcca gttctgggcg gcgattggcc agcacccgtc ccaccacaaa
     1021 gcacaggaat tcttcggcca aatccatgat gtcattcaag tcagcatagg caacctcggg
     1081 ttcaaccatc caaaactcgg tcaagtgacg gcgggtcttg gatttttcag cccggaaggt
     1141 tgggccaaag caataggttt tgccaaaggc ggcagccgtt gcttcgttgt aaagctggcc
     1201 agactgagtc agataggctt tgttatcatc aaaatagtca acttcaaaaa gagttgtggt
     1261 gccttcgcag gcagccgggg tcaggattgg ggtatcagcc agaataaacc catttgaatc
     1321 aaaaaaatcg cgcacggctt tcacaatttc gtggcgcacg cgcaaaatgg cgtgttgacg
     1381 acgtgatcga agccacaagt ggcggtggtc catcaggaac tcaacgccat gttcttttgg
     1441 cgtaatcgga taattttcgg taagctggac cagttcaatg ttttggacgt ccagttcata
     1501 cacgccaggt cgtttgggat gttctcgcgg tactccagtc acgataattg aagattcctg
     1561 ggtcagtttt tcagcggttt cccacacggt ttcagtcact gcttttttga acacaattgt
     1621 ctgaatgaga ccggtgccat cgcgaacttc caaaaattgc agctttccac tggagcgtga
     1681 gttgtaaagc cagccgcgaa gggtgacttc ttgccccaga tgttgagcga tgtcagcaat
     1741 ataagtttgt ttcatgagct ttgatttcca gttgaagtgc ggaatttcgt tgagggcaat
     1801 aaaaaatggt cacgcatgcc ctcgttt
//
LOCUS       DAOQDO010001463         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66635_length_1827_cov_3.163093, whole genome shotgun sequence.
ACCESSION   DAOQDO010001463 DAOQDO010000000
VERSION     DAOQDO010001463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66635_length_1827_cov_3.163093"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKZ53_19785"
     CDS             complement(<1..271)
                     /locus_tag="PKZ53_19785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42739.1"
                     /translation="MTNILIIEDNFDVRQLIKGEVEDLVDQLFESEDGSQAFELYQKH
                     HPDWVLMDLSIKDVDGLTTTRQIKSVYPEALICIVTNYDDEYLREE"
     gene            complement(523..1176)
                     /locus_tag="PKZ53_19790"
     CDS             complement(523..1176)
                     /locus_tag="PKZ53_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ42740.1"
                     /translation="MSNPIRVIIADDHPIVRQGLRHTIEADPQLSVICDVGDGQAALE
                     RVEALKPDVVVLDIDMPKMDGLQVARSIVAKRLKVEIIFLTIHSEEDLFHAALDLGAK
                     GYILKDCVIDDVVSCIKAVINGKYFTSISMTNYLLNRFEPQSPLEPRRRELDLLTPTE
                     LRILKLLGDYKTSKEIAADLNISPRTVETHRNNICQKLDLHGSHALVKFAIQNRKKF"
     gene            complement(1173..>1827)
                     /locus_tag="PKZ53_19795"
     CDS             complement(1173..>1827)
                     /locus_tag="PKZ53_19795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ42741.1"
                     /translation="EAFSRKLIDSQELERKRIAAELHDSLGQSLVIIKNRARLGLKPD
                     DDLENKENQLDGISMAATQAIEELREISFNLRPYLLDKIGLTRTLKSMLGKVFPPGET
                     RVIVEIDELDDVFSKDSEILFYRIIQESVNNIVKHAQATQAHISIKRVKQSVVVTIED
                     NGKGFDLTDTRLTTQQSFGLIGIAERARLLGGNHFIISEIGRGTTVTITIDLSQENL"
BASE COUNT          429 a          457 c          462 g          479 t
ORIGIN      
        1 cttcttcgcg caggtattca tcgtcatagt ttgtaacgat acaaatgagc gcttccgggt
       61 agacagattt gatctggcgg gtcgttgtca gcccgtctac atccttgatt gacaaatcca
      121 tcaacaccca atccggatga tgtttttggt acagttcaaa tgcctgagag ccatcttcgc
      181 tctcaaagag ttgatccacc aggtcttcaa cttcgccctt gatgagttgt ctgacgtcga
      241 aattgtcttc aatgatgagt atgtttgtca tgagtctccg cctttgggtg aggtgggcag
      301 agtatggaca aactcaggcc ggatgagaat tcgcaagact acgcagaatc gctacgtaga
      361 agtacttaaa ttttactgat caaaacagag attctggcgc acatgcgcca ggataagaga
      421 ttttaggccc gcagggccgt tatgtaatag ccgtggtgcg aagcccacgg tcatagtagt
      481 ggggttgcac ctgagcgacg agccgccacc cgcttcacgg gatcaaaact tcttgcgatt
      541 ctggatggca aatttcacca gggcgtgact gccgtgcagg tcgagtttct ggcagatgtt
      601 gttgcggtgg gtttcaaccg tacgcgggct gatattgagg tcagcggcaa tttctttgct
      661 ggttttataa tccccgagca atttcaagat ccgaagctca gtcggcgtca acaaatcgag
      721 ttcccggcgg cgcggttcaa gcggtgactg cggttcaaag cggttgagga ggtaattggt
      781 cattgaaata ctcgtaaagt attttccatt gatcactgcc ttgatgcagc tcacaacatc
      841 gtcaatcaca caatctttga ggatatagcc cttggccccc aaatcgagtg ccgcgtggaa
      901 cagatcttcc tcgctgtgga tcgtgaggaa aataatctcg acctttaagc gtttggccac
      961 aatcgagcgg gcgacctgca aaccatccat cttgggcata tcaatatcaa gtaccaccac
     1021 atccggcttc aaggcttcga cccgctccag ggccgcctgg ccatcgccga cgtcacaaat
     1081 caccgaaagc tgtggatcgg cttcgatggt atggcgtagc ccctggcgca caattggatg
     1141 gtcgtcggca ataatgactc gaatcgggtt gctcatagat tctcctggct aaggtcaatg
     1201 gtaatggtga cagtggtccc cctgccgatt tcggaaatga tgaaatggtt gcctccgagc
     1261 aatcgcgctc gttcggcaat cccaatcaaa ccgaaacttt gctgtgtggt caacctggta
     1321 tcggtcaggt caaatccttt tccattgtct tcgatggtca ccaccaccga ctgtttcacc
     1381 cgtttgatcg agatatgcgc ctgcgtcgcc tgggcatgtt tgacaatgtt gttcacgctt
     1441 tcctgaataa tccgataaaa cagaatttcc gagtctttcg aaaacacatc gtccagttca
     1501 tcaatttcaa cgatcactct ggtttcaccg ggcggaaaca ccttgccgag cattgatttg
     1561 agcgtgcggg tgagaccgat tttatccagc agatatggcc gaagattaaa cgaaatttcg
     1621 cgcaactcct cgatagcctg ggtggcggcc attgagatgc catcgagctg gttttccttg
     1681 ttttcgagat catcatctgg ttttaagccc agacgagcac ggtttttgat gatcaccagc
     1741 gattgaccca atgaatcgtg gagttcagcc gcaatgcgtt tgcgctccag ttcctgtgaa
     1801 tcaatcagct tgcgggaaaa ggcttcc
//
LOCUS       DAOQDO010001464         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66642_length_1827_cov_2.727427, whole genome shotgun sequence.
ACCESSION   DAOQDO010001464 DAOQDO010000000
VERSION     DAOQDO010001464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66642_length_1827_cov_2.727427"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1827)
                     /locus_tag="PKZ53_19800"
     CDS             complement(<1..>1827)
                     /locus_tag="PKZ53_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42742.1"
                     /translation="RDGLPAGWCHRPRRDRIDIDQRPLQRPLLSQVMAQLASSGRIPH
                     FGALAKSDGCVESVADLIGEIQRAGKASGAFRLIVEDRIRRAMESFPKPEDDDDLING
                     LPPEVDAMLGYERDVALIYECYQSALYTNGLTDINEDHLRVLQVLDGEYEGYLCHVPY
                     LKPVRLMVVDGFFDFTPAQGEILKRLIARTPEVFFNFDYDVNNRDVFRPIDDTLEHVE
                     KMADHFERRYFHHIDPTAQPISVEEVLPGLDPIRQALFNTKWEAPPAEAMANLVEEGL
                     PITVFEAPDREREVRAVAKEIKSLVRVESFQPFEIAVVVRNKEAYGQDLRRVFSEEGI
                     AYALDERLKVSDIPAVRAVRKLLDAAASRHPDDTAERPPRIPVGKLVGLLKSDYFHLQ
                     GITSITPDSVYSITSPTDLTVDDIENIVAFVGQQLHLDDWLSRSAKLLGKFDIPVTGK
                     LIGTDQPGLPFEELNLYQEFFEDFEETLLSTDPHPRHQVRADAVIPWQIFATARVLAV
                     LGRILLKIPYESLAQDLVIRLNEVLTDLQFKAHLEQALKEAQGDPEKMLRATLDLRGS
                     EAINRAMAAVQDAVSHAAEGIRIICGGGSAPLKTKGSDLNEAT"
BASE COUNT          396 a          484 c          523 g          424 t
ORIGIN      
        1 ggtggcttca tttaggtcag agcctttggt tttgagcggg gccgaaccgc cgccgcaaat
       61 gatccgaatg ccttcagccg cgtgggaaac cgcgtcctgg accgccgcca tggcccggtt
      121 gattgcttct gacccgcgca ggtcaagtgt ggcccggagc attttttccg gatcgccctg
      181 agcctctttg agcgcctgtt ccagatgggc tttaaattgc agatcggtga gcacttcgtt
      241 gagtcggatg accagatcct gggccagtga ttcatacgga attttaagca gaatacgtcc
      301 aaggacagcc agaactcgtg cggttgcaaa gatttgccag ggaatgactg catcggcgcg
      361 aacctgatga cgtgggtgtg ggtcggtcga aagcagcgtt tcttcgaaat cttcaaagaa
      421 ttcctggtac agattgagtt cttcaaatgg caggcccggc tggtcggtcc caatcagttt
      481 tccggtcacc ggaatgtcaa atttgccaag cagtttagcg gaacgtgaga gccagtcatc
      541 cagatgtagt tgttgcccga caaacgccac gatgttttca atatcgtcaa cggtcaggtc
      601 ggtcggtgat gtgatcgaat acacgctatc gggcgtgatg ctcgtgatgc cctgaaggtg
      661 gaaataatcg cttttgagca ggccgacaag tttgccaacc gggattcgcg gtggacgttc
      721 cgctgtgtca tccggatggc gcgatgcagc cgcatccagc agtttacgaa cggctcgaac
      781 ggctggaata tcagagactt tcaggcgttc atccagcgcg taggcaatgc cttcttcact
      841 gaaaacacgg cgcaggtcct ggccataggc ttccttgttg cgcaccacga cggcaatttc
      901 aaatggttga aaactttcca ccctcaccag cgatttgatt tctttggcga cggcacggac
      961 ttcgcgttcc cggtcaggtg cttcaaaaac ggtgatgggc agaccttctt ccaccagatt
     1021 cgccattgct tcggcgggtg gggcttccca tttcgtgtta aacaatgcct gccggattgg
     1081 atccaaccct ggaagtactt cttcaaccga aatcggttgg gcggtcgggt caatgtggtg
     1141 aaaatagcgg cgctcgaagt gatccgccat tttttcaacg tgttcgagcg tgtcgtcaat
     1201 cggacggaac acgtcgcggt tgttcacatc atagtcaaag ttgaagaaga cttccggggt
     1261 gcgggcaatc agccgcttga ggatttcacc ctgggctggc gtgaagtcga aaaagccatc
     1321 caccaccatc aacctgactg gcttgaggta gggaacgtgg cacaggtagc cttcatattc
     1381 accatcaagc acctgcaaaa cgcgcaggtg atcttcgttg atgtcggtca atccgttggt
     1441 gtaaagcgcc gattggtagc attcataaat cagtgccaca tcacgttcat agccaagcat
     1501 cgcatcaact tcgggtggca ggccattgat caggtcgtcg tcatcttctg gtttgggaaa
     1561 gctttccatt gcccggcgga tgcggtcttc gacaatcaac cgaaaggccc cagacgcttt
     1621 tccggcccgt tggatttcgc caatcaggtc ggccaccgat tcaacacagc cgtccgactt
     1681 ggccagcgcg ccaaagtggg gaatccgtcc ggaacttgca agctgggcca tgacctgtga
     1741 caggagcggg cgctggagcg ggcgctggtc aatatcaatc cggtcacggc gggggcgatg
     1801 acaccagcca gcgggaaggc catctct
//
LOCUS       DAOQDO010001465         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66710_length_1826_cov_3.172784, whole genome shotgun sequence.
ACCESSION   DAOQDO010001465 DAOQDO010000000
VERSION     DAOQDO010001465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66710_length_1826_cov_3.172784"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKZ53_19805"
     CDS             <1..1031
                     /locus_tag="PKZ53_19805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42743.1"
                     /translation="ECGGLTPLSNSCGTRMLTGQTIETYHIGQLLGRSSTAEVYEARQ
                     PRVFNRLVAFKHSLVPVHHSNAANLAFLSEALVAAAVPHPNIVPIFDIGQMPDGEPFF
                     TMQRLTGVTLRFYFEQRLRQNAPVTDKIYSLETRLEHLKACLPILWQVAYGLESIHDA
                     GYLHGNFKPEHVFVHPQGHATLFGFRTHNPEMGLVGAPRYLSPEELRGEPLEYRSDLY
                     AFGVVVHEMLFGHVPFESVTPTEMVLRRLTEPFPAFPNQGAALSPHLEQILQTALNRT
                     LSNRYSSATAFVQDLDQALHNNVIPLRVITPAGHSTTEPMPASVIPFPGPSTLPGPPT
                     QPGDPEVE"
     gene            complement(1158..1454)
                     /locus_tag="PKZ53_19810"
     CDS             complement(1158..1454)
                     /locus_tag="PKZ53_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42744.1"
                     /translation="MKNTGKNYREASENSEKAKYSFSDRYFLGTPASCRHQAVHIQSV
                     AGRIPAFPGGNLFLKSYRQEKRLARENTAQHVQSWQSIYTILEWSPRITGRLKY"
     gene            1496..>1826
                     /locus_tag="PKZ53_19815"
     CDS             1496..>1826
                     /locus_tag="PKZ53_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42745.1"
                     /translation="MTTDQEKIIAMLLGELDEEEHLALHETLLDSQNYEAFKIIQDQL
                     IEDFLEDRLPTERKKRFEKVFLVTPARQSRLKLVQGLKEKQAIRARATNAWWIQLTRW
                     FDLSQPKI"
BASE COUNT          422 a          479 c          455 g          470 t
ORIGIN      
        1 cggagtgcgg tggcttgaca ccgctttcga actcgtgtgg aactcgtatg ttgactggtc
       61 agacgattga aacctatcac atcgggcaac ttcttggccg tagcagtaca gccgaagtct
      121 atgaggcgcg tcaaccccgt gtgtttaatc gtttggtcgc ttttaaacat agtttggttc
      181 cggttcacca ctcaaacgcc gccaacctgg cttttctgag cgaagccctg gtcgcggcgg
      241 cggtccctca tccaaacatc gtgccgattt tcgacattgg ccagatgcct gacggtgagc
      301 cgtttttcac catgcagcgc ctgacggggg taacgttgcg gttttatttt gaacagcggc
      361 tccggcagaa cgcaccggtg accgacaaaa tctattcact tgaaactcga cttgaacacc
      421 tgaaagcgtg ccttccgatc ttgtggcagg ttgcctacgg actcgaaagc atccacgacg
      481 ccgggtatct gcatggaaat ttcaaaccgg agcacgtgtt tgttcacccg caaggacacg
      541 ccaccctgtt tgggttccga actcacaacc ctgaaatggg gctggttggg gcgccccgct
      601 atctttcacc tgaggagttg cgcggagagc cgcttgagta ccggtctgat ttgtatgcgt
      661 ttggcgttgt cgtccacgag atgctgtttg ggcatgtccc gtttgaaagt gtgactccga
      721 ctgaaatggt cctgcggcgg ttaactgaac cgtttccagc ttttcccaat cagggggctg
      781 cgctgtctcc tcacctggaa cagatcctgc aaaccgcgct caatcgaacg ctatcaaacc
      841 ggtattcatc ggcaacggcg tttgtccagg acctggacca ggcgctgcac aacaatgtca
      901 ttccgttgcg cgtcatcaca ccggcgggtc actcaaccac ggaaccgatg ccggcatcgg
      961 tgattccttt tccaggccca tctacgctcc caggaccccc gacccaacct ggcgatcctg
     1021 aagttgaata gtgcacctat tgaactttgc agccaaacgg gccgatgatt cgactttccc
     1081 agacatctgg tgttccctgg ttaataccaa ttagggttca gggtatcggg gtcagggatc
     1141 agggaaatac aattttttca atattttagc cttcccgtaa tacgaggact ccattccaaa
     1201 atggtataaa tggattgcca gctttgaacg tgttgggcag tattttctct tgcaagtcgc
     1261 ttttcctgac gatagctctt cagaaaaaga tttcctcctg ggaatgctgg tatcctgccg
     1321 gcaactgatt gaatatgaac cgcttggtgc cggcaggatg ccggcgttcc caggaaatat
     1381 ctatctgaaa aactatattt cgctttttca ctattttcac tggcttccct gtaatttttt
     1441 ccggtgtttt tcatttctga atagcacgcc gctcaactga actggagacc tctctgtgac
     1501 gactgaccaa gagaaaatta ttgctatgtt gctcggcgaa ctggatgaag aagaacatct
     1561 tgcactccat gaaacacttc tcgacagcca aaattatgag gcgtttaaaa ttattcagga
     1621 tcaactgatt gaggatttcc tggaggatcg gcttccaacc gaacgaaaga aacgctttga
     1681 gaaagtattt ctggtgacac ccgcccgaca atcccgtctg aaactggttc agggattaaa
     1741 ggaaaagcag gccattcgag ccagggcaac caatgcgtgg tggatacagc ttacccggtg
     1801 gtttgacctg agtcagccaa aaattc
//
LOCUS       DAOQDO010001466         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66722_length_1826_cov_2.655562, whole genome shotgun sequence.
ACCESSION   DAOQDO010001466 DAOQDO010000000
VERSION     DAOQDO010001466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66722_length_1826_cov_2.655562"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1691
                     /locus_tag="PKZ53_19820"
     CDS             216..1691
                     /locus_tag="PKZ53_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42746.1"
                     /translation="MIDDSYYILLARALATGQGYTEIHLPVPRAHDHFPPGLPVLLSI
                     PTVFGFSFPTTVLLYKLMLIACGAGALFLFSRLIQAEGYSPLVQSASILLAATSIPFV
                     GFTCRVASEMPFTLFSILALFWFHRFQTEHQKPINGFLVLLFCGLALVTRSIGIVLIV
                     ALFSSWLLAQKHRRLVVFLVVASGIILSSLVWFGSHLPGTREYVDFFLSAYVTRDDAS
                     PLAAIMYYLRENGWLLAWRDIPRAIFSISVSEAIEANQWLKLGATPLRLAISAVVLGG
                     LAYSLRPRIQASSLYVACSLVLIVFWPWNPARFIVPLVPFLCLFFVLGIEFLVRQISQ
                     MKPDLIRNHNQLAGLICLLCILSQLISDIRFVRTVRASGDFSIQAASLWQDTADAYQW
                     LKTQTSEQSVIGCDPPIEAHVYLHTNRKACGLPRRANGYHRLGITHVLKLANPVLHQA
                     DPNTSVNPQQVIDQAKLAGVQLTLVYQNQSVAIFLVHTNLP"
BASE COUNT          410 a          476 c          418 g          522 t
ORIGIN      
        1 agacaacgat gaatgccaaa caaagaatgg ctgcccctca catcacccaa aatctaaacc
       61 attgaaaaaa tcacacttct ccgcaacctt gaaacctgaa tggtactaca atgcttgata
      121 acctcaaaaa gaaccagtat ttcaatttaa tctgtcttct gagtatcata ttctttatta
      181 cggtcggatg tctgatactg aacagtaaat tcgatatgat tgatgactca tattacattt
      241 tactggccag ggcgctggca accggtcaag gatatacaga aatccactta cccgttccac
      301 gggctcacga ccactttccg cctgggttgc cagttttgct gagtattccg acggtgtttg
      361 gtttttcctt tccgacgacc gttcttttgt acaaactcat gttgattgcc tgtggcgccg
      421 gagccctgtt tctcttttcc cgtttgatcc aggccgaggg gtattcgccg ctggttcaat
      481 cggcctcgat ccttctcgcg gcaacatcca ttccgtttgt gggttttacg tgtcgtgttg
      541 cctcggaaat gccgtttaca ctgttttcaa tactcgcgct gttctggttt catcggtttc
      601 aaactgaaca ccagaaacca atcaacggat tccttgtcct gctgttttgc ggcctcgcgc
      661 tggttacccg gtcaatcggg attgtcttaa ttgtagcgtt gttttcaagc tggctgctcg
      721 ctcaaaaaca tcgccgactg gtcgtctttc tggttgtggc cagcggaatt atcctttcca
      781 gcctggtttg gtttggctct cacctccctg gaaccagaga gtatgttgat tttttcctgt
      841 cggcatatgt cacacgggat gatgcttcgc ctctggcggc catcatgtac tacttgagag
      901 aaaacggctg gcttctggcc tggcgtgata ttccacgggc gattttctcg atcagcgtga
      961 gtgaagcgat tgaggccaac cagtggctca aactcggagc cactccgctc agacttgcga
     1021 tttcggcagt ggtcctcgga ggattggcct acagtctgcg ccctcgtatc caggccagtt
     1081 cactgtatgt tgcctgtagt ctggttttga ttgtcttctg gccctggaat ccagcccggt
     1141 ttatcgtccc gctggtgcca ttcctgtgtc tgttttttgt attagggatc gagtttctgg
     1201 tccgccagat ctcccaaatg aagcctgact tgatccgaaa tcacaatcag ctcgctggtt
     1261 tgatttgttt gctctgtatt ttgtcacaac ttatcagcga catcagattt gtgaggacag
     1321 ttcgagcaag tggcgatttt tcaatccagg ctgccagttt gtggcaagac acggcggatg
     1381 cttaccaatg gctcaaaacc caaacctcgg aacaatctgt gataggttgc gatccgccaa
     1441 ttgaagccca tgtgtacctt cataccaacc gcaaagcctg tgggcttccc cgccgagcga
     1501 acgggtatca ccgactggga ataacccatg tgttgaaatt ggcgaatccg gtgcttcatc
     1561 aagctgatcc caacacctcg gtcaatccgc aacaggtgat tgatcaagcc aaactggctg
     1621 gggttcaact cacgctggtc tatcaaaacc agtcagttgc catttttttg gttcacacaa
     1681 accttccatg accactgaat gatatcaact ttgaaaaaag ctttgcctga aaagaaacgg
     1741 taactccagg ccgtgagatt ttaacaaccg cttgttttca ggcaaagcgc ttccacccgg
     1801 tcaagcatcc aaacagagtg gcagct
//
LOCUS       DAOQDO010001467         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66741_length_1826_cov_2.012987, whole genome shotgun sequence.
ACCESSION   DAOQDO010001467 DAOQDO010000000
VERSION     DAOQDO010001467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66741_length_1826_cov_2.012987"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..978)
                     /locus_tag="PKZ53_19825"
     CDS             complement(268..978)
                     /locus_tag="PKZ53_19825"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ42747.1"
                     /translation="MNRLFLRFFLVLTTLLLVSPGLSAQEIQDNQTPILRPDPLTEAI
                     RANDTTTVSELIHNGAVLNPQVVDRTAPLNVAIRFSSLETVEMLVNAGARIDTRWVLP
                     RSEVSYQQMTPLRWSIVSGRHELVRYFLAKGCDPNEVYENQLTPLHVAAERSPEMIEL
                     LLKAKADVTRLDIQGRTPLSVYAANSMDNPKGIDLLLDAGANVNHRDTTQMTPLGWAS
                     ILQHSQKANALVARGGKE"
     gene            1268..>1826
                     /locus_tag="PKZ53_19830"
     CDS             1268..>1826
                     /locus_tag="PKZ53_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42748.1"
                     /translation="MTQIDFGTLSKSHLKLLVLGPTIDAIRPLGALLLEYFWMVELLE
                     YSTLHKSVAHPEVGIVLVDRPDSDSFTWLLDRIQKQYGSLPLVLVIWPEQESSIAHLW
                     EAGQIVDYVYSDRPYRLVSALRRIERDVVMPHRAKREVKPGKSQRQAEERFQDFQSAG
                     SVPLVALDNLNKRLVADNRWAFVVYD"
BASE COUNT          402 a          502 c          461 g          461 t
ORIGIN      
        1 ggttctgttg acattccttc gccgcgtagc ggtggctgac ttgagaccgg tgcttcagcg
       61 ccgggaacta caaccagatt tcggttttaa cctgtgaagt gggtgtcggc acgctagccc
      121 agctctgggc taaaaaccaa accttcaatc caaatcttgt tgtcgtgtta aaacccaatt
      181 ccttccaaga accaggaacc aggaaccaag aaccaggaac caggttcatt ctgcattctc
      241 aattctgcat tctcaattct gcattcttca ttccttgccg ccgcgagcaa ccagcgcgtt
      301 ggctttctgg ctatgttgca ggatgctggc ccatcccagt ggcgtcatct gggtggtatc
      361 ccggtggttg acgttggccc cggcgtcaag cagcaggtca atgcctttgg gattatccat
      421 cgaattggcg gcatacactg agagcggtgt gcgcccttga atatccagcc gcgtgacatc
      481 agctttggct ttgagcaaca gttcgatcat ttccggagag cgttccgccg ccacgtgcag
      541 cggcgttaac tgattttcat acacttcgtt tgggtcacag cctttggcca gaaagtaccg
      601 caccagttca tggcgaccgc tgacgattga ccagcggagc ggagtcattt gttgatacga
      661 aacttcagat cgcggtaaca cccaccgggt gtcaatccgg gcccctgcgt tgaccagcat
      721 ttccacggtt tcgagcgatg aaaatcgaat ggcgacgttg agcggtgccg ttcggtcaac
      781 aacctgggga ttaagcaccg ccccattgtg gatcaactcg cttacggtag ttgtgtcgtt
      841 ggcccgaatc gcttcggtca atgggtccgg acgtaaaatt ggtgtctggt tgtcctgaat
      901 ttcctgagca ctcaggcccg gcgagaccaa aagaagtgtg gtcaagacca gaaagaaacg
      961 taaaaaaaga cgattcattg gttaaatcct cagttaccgc aaaagatagt ggctgcgtca
     1021 cacggcagct tcctccccaa tcagaacctc aaccatagcc gattgaactc caaaagtgaa
     1081 cccaggctca ggcatttggt tgtttgtcag ctcattctat cggttcaacc aatctcaacc
     1141 ccggcatggt ttcggagtca tctctgcatt ttggattctg cattctcaac tctttatcct
     1201 tcattttgcc ccgaatatgg tctaatctag ggacaaccag gccatattcg tttctcgccc
     1261 agagaatatg acccaaatcg acttcggaac cctttccaaa tctcatctca agctccttgt
     1321 cctcggtcca accattgacg caattcgccc gcttggggca ctcctgctgg agtatttctg
     1381 gatggtcgag ttgctagaat actcgacgct tcacaaatcg gttgctcatc cagaagtcgg
     1441 gattgtcctg gttgaccgcc cggattcaga ttcttttacc tggttgcttg atcgcattca
     1501 aaaacaatat gggagcctgc cgctggttct ggtgatctgg cccgagcagg aatccagcat
     1561 tgcccacctg tgggaagctg gacagatcgt ggattacgtt tattctgatc gtccctatcg
     1621 gctggtgtct gccttgcgcc ggattgagcg cgatgtggtg atgccgcacc gtgcaaaacg
     1681 ggaggtcaaa cccggcaaat cccagcgtca ggctgaagaa cgattccagg attttcaaag
     1741 cgcgggttcg gtccctctgg tcgctctcga caacctcaac aagcggctcg tcgccgacaa
     1801 ccgctgggct tttgtcgtct acgacg
//
LOCUS       DAOQDO010001468         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66742_length_1826_cov_1.963298, whole genome shotgun sequence.
ACCESSION   DAOQDO010001468 DAOQDO010000000
VERSION     DAOQDO010001468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66742_length_1826_cov_1.963298"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKZ53_19835"
     CDS             <1..1025
                     /locus_tag="PKZ53_19835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ42749.1"
                     /translation="TFGSVAVNLLSFELDIWGRLRRSTEAARASLLAAEENRKAVITT
                     LVGDVSSSYFVLLELDTELEIARRTLLSRQDSLRLIKLREQGGVATMLDVRQAEQLVY
                     TTTQAMADLERQIEQTENRISLLIGKPPAEVKRGRSLTAQEAPPVVPPGLPSSLLERR
                     PDIRAAEQNLIAANALIGVAKAAYFPRISLTGSIGGQSNQLDTLLSGPSRTWNFIPQL
                     TQPLFAGGRIKSNVKLTEAQRQRAVIEYERTIQTAFREVSDALVQQRKVREIREQREL
                     LVTTLRDRSRLAYLRYRGGVDTQLNALDADRDLFNAELSLAQTRRDELLALVQLYKAL
                     GGGWQQ"
     gene            1609..>1826
                     /locus_tag="PKZ53_19840"
     CDS             1609..>1826
                     /locus_tag="PKZ53_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ42750.1"
                     /translation="MSTQRKPTYTPEEYLDLERAAAYKSEFVNGEIFAMAGASPQHVL
                     IVSNLVRELGVALKEGPCLVFSTDLKVRV"
BASE COUNT          469 a          473 c          485 g          399 t
ORIGIN      
        1 ggacattcgg cagcgtcgcc gtcaacctgc tgtcgtttga actggatatt tggggacggt
       61 tgcggcgttc gaccgaagcc gcccgggcca gtttgctggc cgccgaagag aatcgaaaag
      121 ccgtcatcac cacgctggtc ggagacgtca gctcctcgta ttttgtgctt ctggaactgg
      181 ataccgagct tgaaattgcc agacggacgc tgctttcgcg gcaggattcg ttgcggttga
      241 tcaagctgcg tgaacagggt ggcgtggcga ccatgcttga tgtgcgtcag gccgaacaac
      301 tggtctacac cacaacccag gcaatggcgg atctggagcg gcaaatcgaa caaaccgaaa
      361 accgaatcag tctccttatc ggcaaaccgc ctgccgaagt caaacgcggt cgcagcctga
      421 ccgcacagga agcgccgcca gtcgtgccac caggattgcc gtcttcgctg cttgaacgcc
      481 gtccggacat ccgcgctgcc gaacagaatt tgattgccgc caacgccctc atcggcgttg
      541 ccaaagccgc ctacttcccc cgaatcagtt taacgggcag catcggaggc caaagcaacc
      601 agcttgatac cttgttgagc ggtccaagcc gaacctggaa ctttattccg cagttaaccc
      661 agccgttgtt tgcgggaggc cgcatcaaat caaatgtcaa acttacagag gcgcaacgtc
      721 aacgggcagt catcgaatat gaacgaacca tacaaaccgc ttttcgggaa gtatcagatg
      781 ccctggtcca gcaacgcaaa gtacgggaaa tccgagaaca gcgggaattg ctggtcacga
      841 cgctccgcga ccgctcccgg ctggcctatt tacggtatcg aggcggggtg gacacccaac
      901 tcaacgcttt agacgctgac cgtgacctgt tcaacgcgga gctgagtctg gcacaaaccc
      961 gacgggatga attactggcc ctggttcagc tctacaaagc gctcggtggc ggctggcagc
     1021 agtgagagaa aatgcagaat gaagaattga gaatgcagaa tgaagaatga aagctttcaa
     1081 cgggaataag gtgttgctgt ccaataaaat agattgattt ttaacctgga gtccgcatcc
     1141 ttgactgggt gtccttttca accgattggt tgagaaagga cacccatttt tttcttggag
     1201 gtgttatcat ccaagccatc aactcactcc agtcaaaaga gaaggacaca aacaaggttg
     1261 gcacagaaat gagtgatgaa ctaaggccgg aatatgactt gcgtgtccga aagcctgtcc
     1321 ttctcacgat cagggccagt tttcagtcat tcaaattcgg ttcggtcagg atgaggtttc
     1381 gactatcagg aatgacagct tgtgaggtaa tgatatgacc acaaaagcaa gagcaacaat
     1441 tgatgatctt tatagaatcg ggcaagcgaa ccagaaacac caactgttta tcggcatggc
     1501 gaactcgctg aagccgaacc cgccgtacct ggatggagca tggcggttga tgatctgttt
     1561 tcataaccct ggatagattc tggacaggca aatcaggctc attttcttat gtcaacccaa
     1621 cgcaaaccca cctatacccc tgaagaatat ctcgatctgg agcgggctgc tgcttacaaa
     1681 agcgagtttg tgaacggcga gatttttgcc atggccggcg caagtcctca acacgttttg
     1741 attgtctcaa atctggttcg tgaactgggc gttgccctga aagaaggacc ctgtctggtc
     1801 ttctcaacag atttaaaagt ccgggt
//
LOCUS       DAOQDO010001469         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66767_length_1825_cov_3.845763, whole genome shotgun sequence.
ACCESSION   DAOQDO010001469 DAOQDO010000000
VERSION     DAOQDO010001469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66767_length_1825_cov_3.845763"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="PKZ53_19845"
     CDS             complement(<1..1803)
                     /locus_tag="PKZ53_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42751.1"
                     /translation="MGDDWYKVASNYGVWLTLDPKRTRDASRYLLAAAEDAPKSDEAQ
                     RGLANFHRLDKRFDLAKAHIQLAKELDPAQIETYALEGSILYESGDRTGARAVWNQML
                     NLPRGVSEAYVRYLEVMTEHDLFLEALPAVEDRLAARIRRPDVDPEDWAGLVRAMSAT
                     ADEEPKFAKAVNDSFVRVMKAVPEDMNFGLLILNEKLVPASEKIPFLRMMHERAADTI
                     LAAYSNGDYNFNNYSYYTYYFNYSSPANVLKVVEQRWIDHLLEKKNYSEAEKTLGEMR
                     SLRTLLFEQLRESDAVNPDEYYSDSNELLVEPTWMVMAQAVLELRTNRAAQALQRLRT
                     AMGLDKADDFREGEMEYEGNEYSSDSETKVTYQNAYTLLVNENHQAEADALLGEFYQK
                     FQSPNSPLSFFVGMAEIEFRRGRTTEGLAQLTRLINNRNTADAFISAAEIAAHYGQFA
                     QAVEWRTKATRLAPLNTLNRIELARVQERVGTADAALTTLADLIDNQSTTNPERLTAI
                     EVLGGIARRNRSAASTLMARYTSKPEYTAQLVLAQIQEATGNIGAAKSILDRLTPAPF
                     ATMARIERGQLAIREKQPETALTAYLAAIKDDPAS"
BASE COUNT          359 a          481 c          511 g          474 t
ORIGIN      
        1 gctcgccgga tcatctttga tcgctgccag ataggcagtg agggctgttt cgggttgttt
       61 ttcacgaatt gccagttgcc cgcgttcaat ccgggccatt gtggcaaacg gggcgggtgt
      121 gagccgatcc agaatggatt tggcagcacc gatgttcccg gttgcttcct ggatctgagc
      181 cagcacgagt tgggcggtgt attcgggttt gctggtatac cgggccatca gggttgacgc
      241 tgcgctccga ttgcgacggg caatgccacc caggacttca atcgccgtca atcgctcggg
      301 attggttgta ctctggttat caatcagatc ggccagtgtg gtcagggccg catcggctgt
      361 gccgacacgt tcctgaactc gcgccagttc gatccgattg agggtgttga gcggggccag
      421 tcgggtggct ttggttcgcc attcaaccgc ctgggcaaac tgtccatagt gcgccgcgat
      481 ttctgcggct gaaatgaacg catcggcggt gttgcgattg ttgatcaggc gggttagctg
      541 ggcgagacct tcggtcgttc gaccccggcg aaactcaatt tcagccattc ccacaaagaa
      601 gctcagtgga gagtttggcg attggaattt ctgatagaac tcacccaaca gggcatcggc
      661 ttcggcctga tggttttcat tcacgagcag ggtataggcg ttttgatagg tgaccttcgt
      721 ttctgagtca gaactatact cgttcccctc atactccatt tcaccttccc gaaaatcatc
      781 agctttgtcg agccccatcg cggtgcggag tcgttgcagt gcctgggcag cccgattggt
      841 ccgcaattca agcacggcct gggccatcac catccaggtt ggttcaacca gcaactcgtt
      901 ggaatcggaa taatattcat ctggattgac ggcatcgctc tcacgcagtt gctcaaagag
      961 cagtgtgcgg agcgaacgca tttcacccag cgttttttca gcttccgaat agtttttctt
     1021 ttccagcaag tggtcaatcc agcgttgttc gacgactttc aacacattgg ctggtgaact
     1081 gtaattaaag taataggtgt agtaactgta gttattgaag ttatagtccc cgtttgagta
     1141 agcggcgagt atcgtatctg ccgcccgctc gtgcatcatc cgcaggaacg ggattttttc
     1201 gctggcgggc accagttttt cattcaggat caagaggcca aagttcatat cttccggcac
     1261 ggctttcatg acccgaacaa aactgtcatt gaccgctttg gcaaattttg gctcttcatc
     1321 ggctgtggcg ctcatcgccc ggaccagccc tgcccagtct tcggggtcaa cgtcagggcg
     1381 tcggatccgt gctgccagcc gatcttcaac tgccggcagg gcttcgagaa acagatcgtg
     1441 ttcggtcata acttccagat accggacata ggcttcgctg acgccacggg gcagattgag
     1501 catttgattc cacactgccc gtgcgccggt acggtcacct gattcataca gaatcgagcc
     1561 ttcaagggca taggtttcaa tctgggcagg atctaactct tttgccagtt ggatatgggc
     1621 tttggccagg tcaaatcgtt tgtcgagccg gtggaaattt gccagcccgc gttgtgcttc
     1681 atccgattta ggggcgtctt cggctgccgc cagcagatac cggctggcat ccctggtgcg
     1741 tttgggatcg agcgttaacc agacgccata gtttgaagcg accttgtacc agtcatcacc
     1801 catcagttca cgttgctgat ttggg
//
LOCUS       DAOQDO010001470         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66775_length_1825_cov_3.084181, whole genome shotgun sequence.
ACCESSION   DAOQDO010001470 DAOQDO010000000
VERSION     DAOQDO010001470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66775_length_1825_cov_3.084181"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PKZ53_19850"
     CDS             <1..645
                     /locus_tag="PKZ53_19850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ42752.1"
                     /translation="IHTIYNGFDPADFENLEPVRSHEDEFRLSYVGSLSLKPRRTPEI
                     LLRAVQSLALKIPAFREKCRLEFVGLVYDVPLEQMLQEYNVASMCRIVGIVSHKTAID
                     CMRNADVLVLLINFDQKFGNDSVLSGKFGEYVAAQKPLLAIVPDGEAAHLVRTHHLGM
                     VGAPDDPDEVETAILSLFSQWQAGRLCPPPSEKLVRQFNRYEQTRALATIFSAL"
     gene            817..>1825
                     /locus_tag="PKZ53_19855"
     CDS             817..>1825
                     /locus_tag="PKZ53_19855"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate-resistant acid phosphatase type 5
                     family protein"
                     /protein_id="HNJ42753.1"
                     /translation="MRNHKNYLLFLVWLSVLMAIGCQSTTTPPPPVAPISTAPPKPEP
                     PPIPELEGNQGVQFLAFGDFGTGLADQKLVAEAMAAKASRAPIQFALALGDNFYPAGV
                     ESADDPQWQAKFESIYSQPSLNVDFYAVLGNHDHRLNPDAEVEYSKKPGTRWKMPDHY
                     YAFSKKIDDQTEARFLALDTDPLSEEPEKPVTPAQINQQLSWLESELKKAAAQKSPKI
                     VWTIVFGHHPIVSGGAHGSTRSEHMVKVDKLLKKYGVDVYIAGHDHDLQHIVFEKMNY
                     VISGGGSMTRKVGLTPFTKYAAGELGFAWFRLTPTVLNLEIFAGRDHLHQIGYTHSIL
                     AK"
BASE COUNT          454 a          456 c          455 g          460 t
ORIGIN      
        1 attcatacga tttacaatgg atttgatccg gcggactttg agaatctgga acccgtccgc
       61 tcgcacgaag acgaattcag gctttcctac gtcggctcac tttcgcttaa acctcgccga
      121 acaccagaga tcctgttacg ggccgttcaa tcgctggctt tgaaaatccc agctttccga
      181 gaaaaatgcc gtcttgagtt tgtcggtctg gtctatgatg ttccgctgga acagatgctc
      241 caggaatata acgtggcatc aatgtgccgg attgtgggta ttgtttcaca caagacagcc
      301 attgactgta tgcgaaacgc cgatgttttg gtgctcctga tcaattttga tcaaaaattt
      361 gggaacgata gtgtcctgag tgggaaattt ggggaatatg ttgccgccca aaaaccgctg
      421 ctggcgattg ttcccgacgg agaagccgca catctggttc gtacccatca cctgggaatg
      481 gttggggctc ctgatgaccc ggatgaagtc gaaacagcga ttctatcgct gttttcacag
      541 tggcaggctg gcaggctttg tccacctccg tctgaaaaac tcgtgcgtca gtttaaccgc
      601 tatgaacaaa cacgtgccct ggccacgatt ttctccgctc tttaaaccgc tgagcgattc
      661 tggccagagg gtgtttccaa tgagttgttg caagttcgcc tggaacaaga tatttgtttg
      721 aggacacatc cacagtacag cacccccggt ttatccttga gaataagcgc atcccaattt
      781 tgaacccact ttaaccctgc tttgtatttt gaaaccatgc ggaaccacaa aaattacctg
      841 ctttttcttg tttggctgag tgtgttgatg gccattggtt gccagtcaac cacgactccg
      901 ccaccgccag ttgctccgat ttcgacggcc ccacccaaac ccgaaccgcc accgatcccg
      961 gaacttgaag gaaatcaagg cgtccagttt ctggcctttg gcgattttgg aactggtctg
     1021 gcagatcaaa aactggtggc tgaagccatg gccgccaaag ccagtcgggc gccaatccag
     1081 tttgccctgg ctctgggaga taatttttac cctgccggag tggagagtgc tgacgacccg
     1141 caatggcagg caaagtttga gtctatctac tcacaaccga gcctgaatgt tgatttttac
     1201 gccgtcctgg ggaaccacga ccaccggctc aacccggatg ccgaggttga gtactcaaaa
     1261 aaacccggta cccggtggaa aatgccggat cattattatg ccttctcgaa aaaaattgat
     1321 gatcagacag aagcccggtt tctggcgctt gatacggatc cgttatctga agaacctgaa
     1381 aaaccagtga caccggctca aatcaatcag cagttgagct ggctggaatc tgagttgaaa
     1441 aaagcggcgg ctcaaaagtc tccgaaaatt gtctggacca ttgtgtttgg acatcacccg
     1501 attgtttcgg gaggggcgca tggttcaacc cggagcgagc acatggtgaa ggttgataaa
     1561 ttactgaaaa aatacggggt tgacgtctat atcgccggcc atgaccacga tctgcaacac
     1621 attgtttttg aaaaaatgaa ttacgttatc agtggtggcg gctcgatgac ccgaaaagtt
     1681 ggcctgacgc catttaccaa atatgcggct ggcgaactcg gatttgcctg gtttcgactc
     1741 accccgacgg tactaaacct ggaaattttt gccgggagag atcatttaca tcagattggg
     1801 tatacgcatt cgatactggc caaat
//
LOCUS       DAOQDO010001471         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66777_length_1825_cov_2.879661, whole genome shotgun sequence.
ACCESSION   DAOQDO010001471 DAOQDO010000000
VERSION     DAOQDO010001471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66777_length_1825_cov_2.879661"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..329)
                     /locus_tag="PKZ53_19860"
     CDS             complement(60..329)
                     /locus_tag="PKZ53_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42754.1"
                     /translation="MKTIFKIIIFLILLMIGVGLLFKLLGFVVWIAAVAGMIALIAGL
                     LYVLFGPNVNSQANQLESRTALDAKSVQTELHRRQQEIKNQQNRQ"
     gene            449..1798
                     /locus_tag="PKZ53_19865"
     CDS             449..1798
                     /locus_tag="PKZ53_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HNJ42755.1"
                     /translation="MRLTLSSETLSPALAQLSQANHTLAHHFPGESGHRQPVHSVYGG
                     GHLFKAEAASKLGKLALASLDQFAPESDQLGEALGLTLEKNVLDIIYQRVVEKLRHEP
                     VEDYRIDFEDGYGIRSDDEEDGHVVAAASELAKGVHNQSLPPFIGFRIKALTPEMHSR
                     AIRTLDLFLTTFTQETNGQLPENFCVTLPKVTLPEQVTALVQLLDQLETRLGLPSGAI
                     KIEVMIETTQSLFDAEGRFVPPGLLAAAAGRCLAAHFGAYDYTAGCNITSTYQSLQHP
                     ACDFARHMMQVGFSGTGVWLVDGATNVMPIPPHRTSTGQLLTDDQKAENRQTVHRAWK
                     LHFDNIYHALKSGFYQGWDLHPAQLPVRYAATYLFFFEGLEPAAERLRNFVHKAAQAT
                     RVRDVFDDAATGQGLLNFFLRAINCGALTEAETIERTGLSHEELRSRSFLKILQNRK"
BASE COUNT          447 a          475 c          437 g          466 t
ORIGIN      
        1 ttcattcttc attcttcatt cttcattctt cattcttcat tcttcattct caattggtat
       61 tactgacggt tttgctggtt cttaatctcc tgttgccggc ggtggagttc ggtttggaca
      121 cttttcgcgt caagggccgt tcgggattca agttgattgg cttgcgaatt gacattcggt
      181 ccaaaaagca cgtacaacaa cccggcaatc aacgcaatca ttcccgcaac ggctgcaatc
      241 caaaccacaa accccaacaa tttaaacaac aacccaacac cgatcatgag taaaatcaag
      301 aagatgataa tcttgaagat cgtcttcatg gcttgcctcc gctctttatt gtcaactcat
      361 tggcgacata ctacttcagg ctctgatgtt tggatatttg tttcaacctg aaaagtgcca
      421 ttttcacaca aggagtcatt gctcaaccat gcgcctcaca ctttcatccg aaacactctc
      481 accagccctg gcccaactca gccaggccaa tcacacactg gcacaccact ttccgggcga
      541 atcaggtcac cggcagccag ttcattccgt ctatggtgga ggacatttat tcaaagctga
      601 agcggcttca aaactgggaa aattggccct ggcgtcactc gatcaatttg ccccagagag
      661 cgatcagttg ggcgaagcct tgggactgac ccttgaaaaa aatgtgctgg atatcattta
      721 tcagcgggtc gttgagaagc tccgccacga gcccgttgag gattatcgga ttgattttga
      781 ggatgggtat ggaatccgtt cagatgatga agaagacggg cacgtcgtgg ccgcagccag
      841 cgaactggcc aaaggtgttc acaaccagtc gcttccgcca tttattgggt ttcgcatcaa
      901 agccctgaca cctgaaatgc acagccgggc catcagaacc ctggatctgt ttttgaccac
      961 gtttacccag gaaacaaacg gccagttacc tgagaacttt tgtgtaactt tgcccaaagt
     1021 tacgcttccg gaacaggtga cagcgctggt tcagttgctt gatcagcttg aaacccgcct
     1081 tgggcttcct tcgggtgcca ttaaaatcga agtaatgatt gaaaccaccc aatccctgtt
     1141 tgatgccgag ggtcggtttg ttccacctgg attgcttgcg gctgcggctg gacggtgtct
     1201 ggcggctcat tttggtgcct atgactacac agcaggctgt aatatcacgt caacctatca
     1261 gtcgctccag catccggcct gtgatttcgc ccggcacatg atgcaggtcg gattttcagg
     1321 aactggagtg tggctggttg atggtgcaac caatgtcatg cccatccccc cacatcgtac
     1381 cagcacgggt caactcctga cagatgacca gaaggcagaa aaccggcaga cggtccaccg
     1441 ggcctggaaa ctccactttg acaacattta ccacgcgctc aaaagtgggt tctatcaagg
     1501 atgggattta catccggcac aattacccgt tcgatacgca gcgacctatt tatttttctt
     1561 tgaaggactt gaaccggcgg ctgagcggtt gcggaatttt gtccacaaag cagcacaggc
     1621 aactcgggta agagatgtgt ttgacgatgc agcaaccggc cagggattgc tcaatttttt
     1681 cctccgggcg atcaactgcg gggcactcac tgaagctgaa accattgaaa ggacaggtct
     1741 ttcccacgaa gagcttcgaa gtcgctcgtt tctgaagatt ttacagaatc ggaagtaagg
     1801 gttcagggtt cagggttcag ggttt
//
LOCUS       DAOQDO010001472         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66814_length_1824_cov_12.989259, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001472 DAOQDO010000000
VERSION     DAOQDO010001472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66814_length_1824_cov_12.989259"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKZ53_19870"
     CDS             <1..1039
                     /locus_tag="PKZ53_19870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ42756.1"
                     /translation="LSRFEKMATMAAALLHDVGHGPFSHTIEPIARFRHETWTLSLLT
                     DPTSSLNQILTQVDRDLPAAIVHILQGTFSKRFLCQVVSSQFDCDRLDYLLRDSLMTG
                     VSYGHYDLEWLLSSLEVDPAANTLFIRAKGLHAIEEFIQSRNRMFRQLYFHHLIKAAE
                     AVFSGAIERAVELFQAQNLGTDDTTGILMNFLIGNPLTVQQYLSLDDVDILWHLKRWA
                     LSPDPELSDLATRFSDRRMFKTLDIPPDMTDETVEQILHHGQALVAGSPISPHRAVTV
                     QLSRDIPYFRPGSGKHPILVERGSGKSGLADLIDVSEIVASIQPVRLRRLCCVGELRP
                     AISALLKEHQP"
     gene            1201..1773
                     /gene="efp"
                     /locus_tag="PKZ53_19875"
     CDS             1201..1773
                     /gene="efp"
                     /locus_tag="PKZ53_19875"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNJ42757.1"
                     /translation="MITTAEFKRGAQILIDNEPFSIVDYSVQSPSARGASTLYKTKVR
                     HLITGNMFEKTFKAGDRFDEPDVEEREAQYLYESGGEYFFLDQESFEQFGLPTEKMED
                     LIPFLAEEAIVKSVLFNGEVVGVKLPQFLEFEVTEAEPGARGDTATGKVLKNAKISTG
                     ASVKVPIYLEVGERILVDTTTGEFVKRAKS"
BASE COUNT          480 a          460 c          464 g          420 t
ORIGIN      
        1 actgagccga tttgagaaga tggccaccat ggcagcagca ctgctccacg atgtcgggca
       61 cggaccgttt tcgcacacga ttgaaccaat tgccagattc cggcacgaaa cctggacact
      121 ttccctgctc accgatccaa ccagttcgct caaccagatt ctgacccagg ttgatcgtga
      181 cctgccagca gcgattgttc acatcttgca gggcactttt tccaagcggt ttctgtgtca
      241 ggtggtctct tcgcagtttg attgtgaccg gctggattat ctgctacgag attcactaat
      301 gaccggcgtc agctatggcc actatgatct ggaatggctg ttgtcctcgc tggaagttga
      361 cccagcagcc aatacactct ttatccgagc caaagggctg cacgcaattg aagaatttat
      421 ccagtctcgg aaccggatgt ttcgtcaact ctatttccat catttaatta aggcggctga
      481 agccgttttt tccggtgcca ttgaacgggc agttgaactc tttcaggcgc aaaacctggg
      541 tacagacgac acgaccggga ttctgatgaa ttttctcatt ggcaatcccc tgacggttca
      601 gcaatacctg tcactggacg atgttgacat cttatggcat ctcaaacggt gggcattgag
      661 ccctgacccg gagttatctg atctcgcaac ccgattttct gaccggcgaa tgtttaaaac
      721 tctggatata ccgccggata tgacggatga aaccgtcgaa caaatcttgc atcacggtca
      781 ggcactggtg gcaggcagcc cgatttctcc gcaccgggcc gtcaccgttc aactgagccg
      841 ggacattccc tactttcgcc ctggcagcgg aaaacacccg attctggtcg agcgcgggag
      901 cggaaaatca ggactggccg atctgattga tgtctcggaa atcgtggcca gcatccagcc
      961 ggttcgcctg cgccgcctgt gttgtgtcgg agaattgcga ccagctattt cggcattgct
     1021 caaagagcac cagccttaat ttggggggtc agggaaaaac aaaaaagacc aaaaagacca
     1081 aaaggaccaa aattcgaaaa cccggaaccc ggaacccgga acccggaacc cggaacccgg
     1141 aacccggaac ccggaaccct aaatgtctta ttcaactgca ttcaaaggag tatttcgtgc
     1201 atgatcacaa ccgcagaatt taagcgcggt gcgcagattt taattgacaa cgaaccgttc
     1261 agcattgtgg attactcggt ccagagccca tcggcccgag gtgcttcgac gctctacaag
     1321 accaaagttc gtcatctcat tactggaaac atgtttgaga aaacttttaa ggcgggagac
     1381 cgctttgacg agccagatgt ggaggaacgc gaagctcaat atctgtatga aagtggcggt
     1441 gagtattttt tcctggatca ggaatcattt gaacagttcg ggcttcccac tgagaaaatg
     1501 gaagatctga ttccatttct ggccgaagaa gcgattgtca aatcagttct gttcaatggt
     1561 gaagtggtag gggtaaaact gccccagttt ctggaatttg aagtcaccga agccgaaccc
     1621 ggcgctcgcg gagacaccgc aaccgggaaa gtgttgaaaa atgccaaaat ttcaaccggt
     1681 gccagtgtca aagtgccgat ttatcttgaa gttggcgagc gcattctggt ggataccacc
     1741 accggcgagt ttgtcaaacg cgccaaatcc tgaagaatga agaatgaaga atgaagaatg
     1801 aagaatgaag aatgaagaat gaag
//
LOCUS       DAOQDO010001473         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66961_length_1822_cov_4.045274, whole genome shotgun sequence.
ACCESSION   DAOQDO010001473 DAOQDO010000000
VERSION     DAOQDO010001473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66961_length_1822_cov_4.045274"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1057
                     /locus_tag="PKZ53_19880"
     CDS             71..1057
                     /locus_tag="PKZ53_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNJ42758.1"
                     /translation="MRAMIFEKVGQPLRMVELPDPVPGPTQVRLRVLACAVCRTDLHV
                     VDGELPNPKLPLIPGHQIVGIIEAVGQQVTHLKPGQRVGVPWLGSTCGICTFCQRGKE
                     NLCDDAKFTGYHLDGGYAEQTVADERYCFPIPEGYPDLQAAPLLCGGLIGYRALRMTE
                     SAEILGFYGFGSAAHMLIQVARFQGRRVYAFTKKGDTEGQEFARNLGAVWAGDPGQEA
                     PEKLDAAILFAPAGELVPAALRAVTKGGIVVCAGIHMSDIPSFPYRILWEERVIRSVA
                     NLTRQDGEEFLEIAPKVPVHTEVTVYPLEQANEALTDLREGRICGSAVLAVQ"
     gene            complement(1141..1719)
                     /locus_tag="PKZ53_19885"
     CDS             complement(1141..1719)
                     /locus_tag="PKZ53_19885"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ42759.1"
                     /translation="MSANPVHLYTLEEYFALEHASDARYEYWDGEIICMSGGSREHGV
                     ISRNVVGNLFSQLKGSPCEVHTSDSAIKTPVFPPYRYPNASAVCGKPIFENIQGIDVL
                     VNPVLLVEIISPTSEKRDLEKKLEIYQAIPSVKEYLVIYSTKRLVIQFVKQESGKWSR
                     LETSNEQGVVELTSVGCRLEMTEIYDRVFDGV"
BASE COUNT          441 a          455 c          484 g          442 t
ORIGIN      
        1 gaacccggaa cccggaaccc ggaacccgga acccggaacc cggaacccgg aactcctgat
       61 cggtacgatt atgcgagcga tgatttttga gaaagtcggt caaccacttc gaatggttga
      121 actcccagac ccggtgcctg gcccgacgca agtgcggtta cgggttttgg cctgtgccgt
      181 gtgtcggaca gatttgcacg tggtggatgg tgaactcccc aatccaaagc tgccactcat
      241 tcccggtcac caaatcgttg gcattattga agctgtcggg caacaggtca cgcatctcaa
      301 gcctggtcaa cgggttggtg tgccgtggct gggctcgacc tgtggcatct gcacgttttg
      361 tcagcgggga aaagaaaacc tctgcgatga cgccaaattt actggctatc acctcgatgg
      421 tggctatgcc gaacaaactg ttgcggatga acgctattgc ttcccaattc cagaaggcta
      481 cccggatttg caagccgcac cgctgttgtg cggcggactc atcggctatc gggcgttgcg
      541 aatgactgaa tctgccgaaa ttcttgggtt ttacggcttt ggttccgccg cacatatgct
      601 gattcaggtg gcccggtttc aaggtcgtcg ggtgtatgcc ttcaccaaaa aaggcgatac
      661 cgaaggacag gaatttgcgc gaaatttagg ggcggtttgg gccggtgacc caggccagga
      721 agcacccgaa aaactggatg cggcaattct ttttgctccg gcaggagagt tggtgccggc
      781 agcacttcgg gcggtgacca aaggtgggat tgtggtctgt gctggaatcc acatgagtga
      841 cattccgtca tttccatatc ggattttgtg ggaagagcgg gtcattcggt cagttgccaa
      901 cctgacccgt caggatggcg aggaatttct ggaaattgct ccgaaagtgc cagttcacac
      961 tgaggttacg gtatatccac ttgaacaggc caatgaggcc ctgaccgatt tgcgtgaagg
     1021 tcgcatttgt ggaagtgctg tgttggcggt tcagtagagg gaagctgagt tttgggaatg
     1081 cgaaaagaac aaaatttcga aaaccctgaa ccctgaaccc tgaaccctga accctgaacc
     1141 ctaaacccca tcaaataccc gatcataaat ttccgtcatc tcaagtcggc acccaaccga
     1201 agtcagctcc acaactcctt gctcattaga tgtttccagc ctgctccact tgcctgattc
     1261 ctgtttgacg aattgaatca ccagccgttt ggttgaataa atcaccagat actctttgac
     1321 cgaaggaatc gcctggtata tctccagctt tttttccagg tcgcgttttt ctgaggttgg
     1381 ggaaataatc tcaaccagta acaccggatt taccagaaca tcaatccctt gaatattttc
     1441 aaaaataggc ttaccacaaa cggctgaagc atttgggtac cgatatgggg gaaagacggg
     1501 ggttttgatg gctgagtcag aagtatggac ttcacatggt gaacctttca gttggctgaa
     1561 caaattacca acaacgttcc tggagattac accatgctct cgactccctc cgctcatgca
     1621 aatgatttct ccatcccagt attcataccg ggcatcactg gcatgttcga gggcgaaata
     1681 ttcttccaat gtgtagagat gaactggatt ggcagacata tgaaacctct tttgggctga
     1741 aactcctggg gctgagggct tggggctgaa gactcgcaag ctcggggctg aagacaggtc
     1801 caacccaaaa gcttcaggtt aa
//
LOCUS       DAOQDO010001474         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66964_length_1822_cov_3.689870, whole genome shotgun sequence.
ACCESSION   DAOQDO010001474 DAOQDO010000000
VERSION     DAOQDO010001474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66964_length_1822_cov_3.689870"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKZ53_19890"
     CDS             <1..855
                     /locus_tag="PKZ53_19890"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ42760.1"
                     /translation="DTVPPFFGWSEDEEFVYGRGACDAKSIMAAMIVAARQLQAQGTK
                     GFGLLFVVGEERGSIGARRANDIPNVSRFLINGEPTESTLATGSKGALRFKLKTRGVA
                     GHSAYPELGESAIHNLLDVLQTLRQTAWPTCSRLGATTLNIGEIQGGVAPNVIAPQAE
                     AHLIFRVVTSIAHLKQHLESVVGQQAAIEYLFECEPVITEVIEGFPTSIAAFTTDIPL
                     LTNWGKPLLFGPGSIHDAHTATERIRKSELLAAPARYVELVKKLIPEVVSEPQNVNGF
                     QPEFVQFD"
     gene            complement(897..1268)
                     /locus_tag="PKZ53_19895"
     CDS             complement(897..1268)
                     /locus_tag="PKZ53_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42761.1"
                     /translation="MANDADFLGQFPPGSPERQYHGTLLGLINEIDFPRSEAVGLALL
                     RLLQVADFTVGQVDTTIHPAAGSLYENAALVAWLFHTVDSEPIFWIASYQNTGGAVPQ
                     HWFQFWVDQFRSHHNVTSLRV"
     gene            complement(1286..1681)
                     /locus_tag="PKZ53_19900"
     CDS             complement(1286..1681)
                     /locus_tag="PKZ53_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42762.1"
                     /translation="MPHPLTIQNLLSRVPETSEDHQFWKAIYDLLTALSLPQEEAYGY
                     AAILALELADIQLRDGRKIGTFGVEGGIEDLCQVVAVVFASVSPDPGWWKCAYPQKMS
                     HPTVRKILPWLNKKIEELEIVENYSPWIT"
BASE COUNT          485 a          468 c          425 g          444 t
ORIGIN      
        1 gacacggtgc cgccattttt tggctggtcg gaagacgaag aatttgtgta cggacgcggc
       61 gcctgtgacg ccaagagcat catggcagca atgattgtgg cagcccggca actccaggcc
      121 caaggaacca aaggttttgg cctgctgttt gtcgtcggtg aagaacgtgg cagcatcggt
      181 gcccggcgag ccaacgacat cccgaacgtt tcacgatttt taatcaacgg agagccaacc
      241 gaatccacac tggcgactgg ttcaaaaggt gccttgcggt tcaaactcaa aacccgaggc
      301 gtggccgggc attcggctta tccggaactt ggcgaatcag cgattcacaa cctgctcgat
      361 gtcctgcaaa cgcttcgcca gacagcctgg ccgacctgtt cgcgattggg agcaacgaca
      421 ttgaacattg gcgaaatcca gggcggcgtg gcgccaaatg tgattgctcc acaggccgaa
      481 gcacatttga tttttcgagt tgtgaccagt attgcccatc taaaacaaca ccttgaatcg
      541 gtggttggtc aacaggcagc tattgaatat ctctttgaat gcgaaccagt catcaccgag
      601 gtgattgaag gctttccaac ttcgattgcg gcatttacca ctgacattcc gcttttgacc
      661 aactggggga agccgctgtt gtttggcccc ggctcaatcc acgatgccca caccgccacc
      721 gaacgcattc gaaaaagcga attgctggcc gctccggccc gatatgttga attggtgaaa
      781 aaattgattc ctgaagtagt ttcagaacct caaaatgtga atggatttca acctgagttt
      841 gtccagtttg attaaaacag atcgcctttc agattcgatc cgccaataaa ttcaggctat
      901 accctgagtg aagtcacatt atgatgactg cgaaactgat caacccaaaa ctgaaaccag
      961 tgttgaggaa ccgcccctcc tgtgttttgg tagctggcaa tccaaaaaat gggttcagaa
     1021 tcaactgtat gaaaaagcca ggcgacaagc gctgcatttt catacaatga acctgcggct
     1081 ggatgaatag ttgtatcaac ctgcccaact gtaaaatcag cgacttgtaa caacctcagc
     1141 agtgccagcc caactgcttc agaccgagga aaatcaatct cattgatcag gccgagaagc
     1201 gtgccgtgat actggcgttc agggctccca ggaggaaatt ggccgagaaa gtctgcgtca
     1261 ttcgccataa aaacctgcct gatggttaag tgatccaggg gctatagttt tctacgatct
     1321 caagttcttc aattttcttg tttaaccagg gcaagatttt gcgaacggtt ggatggctca
     1381 ttttctgcgg ataagcacac ttccaccacc ctgggtctgg gcttactgag gcaaagacaa
     1441 ccgcaacaac ctgacagaga tcctcaattc caccctcgac cccaaacgtc ccgatcttgc
     1501 ggccatctct caattgaata tcagccagtt caagcgccaa aatggcggca tatccatagg
     1561 cttcttcctg aggcaaggaa agagcagtca acagatcata gattgctttc cagaattgat
     1621 ggtcttcact cgtttcagga acccttgata acaggttttg gatcgttaaa ggatgcggca
     1681 tatcgtttct gaatatctct ggttacggaa cttgttcagt agttccatca gcatcaagta
     1741 tcatcttccc ctcataatct atcatcccat tcccattaaa gatcatccca tcaagtgttt
     1801 cggtaatcgc aaaaatcaaa tc
//
LOCUS       DAOQDO010001475         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66971_length_1822_cov_3.152801, whole genome shotgun sequence.
ACCESSION   DAOQDO010001475 DAOQDO010000000
VERSION     DAOQDO010001475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66971_length_1822_cov_3.152801"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>1822)
                     /locus_tag="PKZ53_19905"
     CDS             complement(47..>1822)
                     /locus_tag="PKZ53_19905"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ42763.1"
                     /translation="NEQKQNYLKAAECLTYLQNQTEERWVKNLVLGSESQKRNLITNQ
                     NNRWYGQTIWFHLHYNTEIPEFGRVALQAILRKKGRALDAMAETISSLRQQSTPEGQA
                     ILDELFVVRSQLSALSLRGPGKTPIEQYQAKLANLEKQTNQLEARLSALSFQYRQSAT
                     PINLDDIQAAIADQTTLIEYCLYLPVSPQARTWGAPRYAVYTLDRSGKIGFADLGERS
                     EIDSLVKEFRQALAAAGDQRNLTKLTLSTARRRSLNQVQKLARQLDAKLLQPVRKFIG
                     EKDHLLLSPDGALNLIPFAALVDESHRYLVESHEITYLSSGRDLLRLKEKVPTNQPAL
                     VLANPKFDDGSGPTIFGQNFDPLAQLPGGEEEGKFLKSLFPKTQLKQGADATETAIKQ
                     ANQPEILHIATHGYFLEPDQATQTLTQALENRSLSLSLSDQTVSPEFVRQSSPLLRSM
                     LFFSGANRQPSTTENTDDGILTALEVASLNLWGTKLVVLSACDTGVGEIKRGDGVYGL
                     RRALVLAGSESQMISLWPVSDAGTRELMFKFYRRLKAGEGRSEALRNAQLDLIKNPKR
                     NHPYFWASFIQSGEWANLDGKRKEE"
BASE COUNT          389 a          447 c          511 g          475 t
ORIGIN      
        1 ttcttcattc ttcattcttc attcttcatt ctcaattctt cattcttcat tcttccttcc
       61 gcttcccgtc cagattggcc cattcgccgg attggataaa actggcccaa aagtagggat
      121 ggttgcgctt tggatttttg atcaggtcaa gctgggcatt gcgaagggct tcgctacggc
      181 cttcgccagc tttgaggcgg cgatagaatt tgaacatcaa ctcgcgggtg ccggcatcgg
      241 aaaccggcca gaggctgatc atctgggatt cgctgccggc cagaaccagt gcccggcgca
      301 ggccgtagac accatcgccg cgtttgattt caccaacccc agtgtcacag gcggaaagga
      361 caaccagttt ggtgccccag aggttgaggc tggcgacttc gagggcggtc aggatgccat
      421 cgtcggtgtt ctcagttgtg gatggctgcc gattggcacc tgaaaagaag agcattgagc
      481 gcaggagcgg gcttgattgc cggacaaact caggcgaaac ggtctggtca gacaggctaa
      541 ggctgaggga gcggttttcg agggcttggg tgagcgtctg agttgcctga tctggctcca
      601 aaaagtagcc gtgggtggca atatggagaa tttccggttg attggcttgt ttgatagctg
      661 tctcagtagc atcggcgcct tgtttcaact gggttttagg aaagagggat ttgaggaatt
      721 tgccttcctc ctccccaccg ggaagttgag ccagtgggtc aaaattctga ccgaaaatgg
      781 ttgggccaga cccgtcatca aatttggggt tggccagcac cagcgccggc tggttcgtcg
      841 gaactttctc tttgagccgg agcaagtcac gcccgctcga gagataagtg atttcgtgcg
      901 attcgaccag gtagcggtgg gattcatcaa ccagtgccgc aaagggaatc aaattgagcg
      961 ctccatccgg agacagcaac aaatgatctt tctcaccgat gaacttccga actggctgca
     1021 ggagcttggc atcaagctgt cgcgccagtt tctgaacctg attcagtgat cggcggcgtg
     1081 cggttgaaag ggtgagtttg gtcaggttgc gctggtcgcc agccgccgcc agcgcctgac
     1141 gaaactcctt gaccaggctg tcaatttcag accgttcccc caaatcagca aacccaattt
     1201 ttccactgcg atccagggta tagaccgcat agcgaggtgc tccccaggtt cgtgcttgag
     1261 gagaaacagg taaatacagg caatattcga tcaaagtcgt ttgatctgcg atggcggcct
     1321 ggatgtcatc caaattgatg ggggtggcag actgacgata ttgaaaactc agggcactga
     1381 gtctggcctc aagctgattg gtctgttttt caaggttggc gagtttggcc tggtactgct
     1441 caatcggggt ttttccgggt ccgcgtagac tcaaagccga aagctgactt cgaacgacaa
     1501 acaattcatc cagtattgcc tggccttcag gtgtgctttg ctgacgcaat gagctgatgg
     1561 tttcggccat ggcatccagg gcacgtccct tttttctcag aattgcctgg agtgcaaccc
     1621 gcccaaactc tggtatttcg gtattgtaat gaaggtgaaa ccagatcgtc tggccatacc
     1681 atctattgtt ctggtttgtg atcaaattgc gtttttgaga ttccgaccca agaaccaggt
     1741 ttttcaccca tctttcttcg gtctgatttt gcaggtaggt aaggcattct gccgccttga
     1801 gataattttg cttttgttcg tt
//
LOCUS       DAOQDO010001476         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_66995_length_1822_cov_2.397849, whole genome shotgun sequence.
ACCESSION   DAOQDO010001476 DAOQDO010000000
VERSION     DAOQDO010001476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66995_length_1822_cov_2.397849"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKZ53_19910"
     CDS             complement(<1..1030)
                     /locus_tag="PKZ53_19910"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNJ42764.1"
                     /translation="MEAQQRLIETNDPHQWVNLAPGFEIYRAAWTEAAQAVLTREAIE
                     LIANLHRAFNPERLELLAARQERQAKIDAGALPAYLPKDSVAVTTDWKVAPIPSDLLR
                     RRVEITGPVNSTKMVINMLSRNAEGDRADTAMLDFEDSMKPSWTNVIQGVHNVIGAIE
                     KTLSYTQPGEKGQPDKVYQLDPADMAVVMVRVRGLHLNESNIRIDGSPIHGGLMDFAL
                     CMLHTAKTLVAQGATPKYYIPKCEHYLEARWWNNLFVAVQQALDIPVGTLRATFLIET
                     LPAAFQMEEILYEIREHAAGLNVGRWDKIFSDIKVLKNHTDRILADRATIGLNRPWMK
                     NYALRLIKI"
     gene            1303..>1822
                     /locus_tag="PKZ53_19915"
     CDS             1303..>1822
                     /locus_tag="PKZ53_19915"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ42765.1"
                     /translation="MAFHADNVNFILGLKLKTFRHEKGFSLKDLAEETGLSVSYLSEI
                     EKGKKYPKPEKILHLATALGVQFDELVSMKVDEALDPLKSILDSPFIQSFPFHLFGVQ
                     PKELLHLIVDAPEKIRALVHTFLEISHVYDVRVEHFLFAALRSYQQMNGNYFEDLETA
                     AMEFRKAQGWQDQ"
BASE COUNT          446 a          470 c          446 g          460 t
ORIGIN      
        1 agatcttgat caggcgcagg gcataatttt tcatccaggg gcggtttaac ccaatcgtgg
       61 ctcgatccgc caggatgcgg tccgtgtggt ttttcaaaac tttgatgtcg ctgaaaatct
      121 tgtcccatcg gcccacattc aacccggcgg cgtgctcgcg gatctcatac agaatttctt
      181 ccatttgaaa ggcagccgga agcgtttcaa tgagaaatgt ggcgcggagc gtgcccactg
      241 gaatgtcgag ggcttgctgg acggcgacaa acaggttgtt ccaccagcga gcttccagat
      301 aatgctcgca tttaggaatg taatactttg gcgtggcacc ctgggcgacc agggttttcg
      361 cggtgtggag catacacagg gcaaaatcca tcaagccgcc gtggatgggg ctgccatcaa
      421 tgcgaatgtt tgattcgttc aaatgcaacc cacgtacgcg aaccatcacc acggccatat
      481 cggcgggatc aagttgatac accttgtcgg gctggccttt ttcacccggc tgggtgtagg
      541 aaagcgtttt ttcaatggct ccgatgacgt tatgaacgcc ctggatcaca ttggtccagg
      601 atggcttcat tgaatcttca aaatccagca ttgccgtgtc ggctcgatca ccttcagcat
      661 ttcggctgag catgttgatg accatcttgg tgctgttgac cggaccagtg atttccaccc
      721 ggcggcgcag caaatccgat ggaatcgggg ccactttcca atcagtggtc acggcaactg
      781 aatcttttgg taaataggcc ggcaaggcgc cggcatcaat ctttgcctga cgttcctgac
      841 gggccgccag caattccagg cgttcaggat tgaaggcccg gtgaagattg gcaatcagtt
      901 caatcgcttc acgagtcaat accgcctgag cggcttcggt ccaggcagcg cgataaattt
      961 caaatccagg agctaaattc acccactgat gtgggtcatt ggtttcgatc aggcgctgtt
     1021 gagcttccat agcggttttt tccccaattc tgcgaaataa agaggcacat ggctgcccct
     1081 cacgtaaagg aagcaagtct caaaccaaac tcagggttga cgaagtgaag tgcaaaagaa
     1141 attttgatga ttgaattgtg cgctgtgatg tggcgcagtg aacgtgcgga atgtatactc
     1201 tgagcgaatt ctctgtgtca agcgaaattt cgctaaattt tccgatttca ctttttgcga
     1261 aatttaaacc ttctgcgaac ttcccatctc tggaatcaca ctatggcctt ccatgccgat
     1321 aatgttaatt ttatcttagg actcaaactc aaaacctttc gccacgaaaa aggcttctca
     1381 ctcaaagatt tagctgaaga aaccggactt tccgtttctt atttgagcga gattgaaaaa
     1441 gggaaaaaat accccaaacc tgaaaaaatc cttcatctgg cgacggcgct tggagtccag
     1501 tttgacgaac tggtctctat gaaagtagac gaagccctcg acccactcaa atccattctg
     1561 gattcaccct ttatccaaag cttccctttt cacctgtttg gcgttcagcc caaggaactc
     1621 ctccacctga ttgtggacgc ccctgaaaaa atccgggcgc tggtccacac ctttcttgag
     1681 atcagccacg tctatgacgt acgcgtcgag catttcctgt tcgccgccct gcgctcatat
     1741 caacaaatga atgggaatta ttttgaagac cttgaaaccg ccgccatgga atttcgcaaa
     1801 gcccagggct ggcaggacca gc
//
LOCUS       DAOQDO010001477         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67185_length_1819_cov_3.375283, whole genome shotgun sequence.
ACCESSION   DAOQDO010001477 DAOQDO010000000
VERSION     DAOQDO010001477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67185_length_1819_cov_3.375283"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKZ53_19920"
     CDS             complement(<1..796)
                     /locus_tag="PKZ53_19920"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNJ42766.1"
                     /translation="MQVDNELIIETPEHVQLRFVLASVGTRFLAALIDHLIQGAAIIT
                     ILILVYAAYNVFGFFKSAGNWAIAAVVFVIGGLILLIIGYFTLFESLWSGQTPGKRWM
                     KLRVIREDGRAVGFFEALVRNMLRVIDLLPSGYAIGVVTIIVSDQSRRIGDYVAGTVV
                     VKERASEAPSLSEVLDRHERETRRNGSNRPALQLDIRQLTKQEFLIVEKFLARRNEIP
                     QQSRPHVAARIAIPLLQKFQVMSVESYEAFLEDVDRQYKANAKYLVD"
     gene            complement(942..1373)
                     /locus_tag="PKZ53_19925"
     CDS             complement(942..1373)
                     /locus_tag="PKZ53_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNJ42767.1"
                     /translation="MVKPIYQDPQVIENILKTCRRVAVVGLSPKPDRPSYGVSEYLLE
                     TGYDVIPVNPTAKEVFGKTCYPNLIAIPDGVDLVTIFRRPEDVPEIVEEAIAKGAKAV
                     WMQFGVINDSAAERAHSAGLDVVMDCCMSVEHGRRRRAGKL"
     gene            complement(1394..>1819)
                     /locus_tag="PKZ53_19930"
     CDS             complement(1394..>1819)
                     /locus_tag="PKZ53_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNJ42768.1"
                     /translation="QRFLDLYGGTTPEPKRVLETPDEDLRKAGISRQKLGYLKDLAAK
                     FEGGIVPVDDLKELDDEAMVEALTTIKGIGRWTVQMFLIRLGRLNVLPDADLGIQKAI
                     KEAYELDSLPAPKEVVKIGAKWQPYCTIASWYLWRSIDG"
BASE COUNT          449 a          489 c          453 g          428 t
ORIGIN      
        1 agtccaccaa atatttggcg ttggctttgt actggcggtc tacatcttcc aggaacgcct
       61 cataactttc aactgacatt acctgaaact tttgcagaag tgggatggca attcgggcgg
      121 cgacatgcgg gcgggactgt tggggaattt cgtttcggcg ggcaaggaat ttttcaacga
      181 tgagaaattc ctgtttggtc aattgacgga tatccagttg caacgctggt cggttgctcc
      241 cattgcggcg ggtttcacgt tcgtgacggt caagcacttc ggaaagtgat ggcgcttcgg
      301 aggcacgttc tttgaccaca actgttccag caacatagtc accaatccgg cgggactggt
      361 cgctgacgat gatggtcaca accccaatcg cataccctga cggcagcaaa tcaatcaccc
      421 ggagcatatt gcggaccagc gcctcgaaaa agccgacggc gcggccatct tcgcggatca
      481 cacggagttt catccagcgt ttccctgggg tttgcccgct ccagaggctt tcaaacagcg
      541 tgaaataccc gataatgagc aaaatcaaac cgccaatcac aaacaccacg gcggcgatag
      601 cccagtttcc ggctgatttg aaaaacccga acacgttata agccgcatat accaggatca
      661 agatagtgat aattgccgca ccctggatca aatggtcaat caacgcagcc agaaaccgcg
      721 ttccgacact ggccagcaca aaccgaagct gaacgtgctc tggggtttca atgatgagtt
      781 cattatcaac ttgcatagcc ggaagtctcc tttgagggtt ggcgtcttaa aaggaccaaa
      841 gggacataaa ggacgaaaaa gacataaaga acaatcattc gaaaatccgg aatccggaac
      901 ccggaacccg gaatccggaa cccggaaccc ggaacccagc tctaaagctt tccagcccga
      961 cggcggcgac catgttcaac cgacatgcag caatccatca cgacatccag cccggcacta
     1021 tgggcgcgct cggcagccga atcgttgatc acgccaaatt gcatccagac agctttggca
     1081 cctttggcaa tggcttcctc gacaatttcc ggaacgtctt ccgggcggcg gaaaatggtg
     1141 accagatcaa ctccgtctgg aatcgcgatc aggtttgggt aacaggtctt cccaaacact
     1201 tctttggcag ttgggttcac cggaattacg tcataaccag tttcaagcag gtattcgctc
     1261 actccataac ttggacgatc tggctttggt gacagcccaa caaccgcaac acgacggcag
     1321 gttttgagaa tgttttcaat gacttgtggg tcttgataga ttggtttcac catagttttt
     1381 gattgtcttc tttctaaccg tcaatgcttc gccacaggta ccacgaagct atcgtgcaat
     1441 aaggctgcca tttggcgccg attttgacca cttcctttgg tgcggggagc gagtcgagtt
     1501 cgtaggcttc cttaatcgcc ttttgaattc ccaaatcagc atctggaagc acgttgagcc
     1561 gccccagtcg gatcagaaac atttgcaccg tccagcggcc aatgcctttg atcgtcgtga
     1621 gggcttcgac catggcttcg tcatcaagtt ccttgagatc gtcaactgga acaattccac
     1681 cttcgaactt ggccgccaga tctttcaggt atccaagttt ctggcgggaa atcccggctt
     1741 tgcggaggtc ttcatcgggc gtttcaagca cgcgtttcgg ttctggcgtt gtaccgccat
     1801 aaaggtctaa aaatcgttg
//
LOCUS       DAOQDO010001478         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67492_length_1815_cov_2.505682, whole genome shotgun sequence.
ACCESSION   DAOQDO010001478 DAOQDO010000000
VERSION     DAOQDO010001478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67492_length_1815_cov_2.505682"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKZ53_19935"
     CDS             <1..297
                     /locus_tag="PKZ53_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42769.1"
                     /translation="HPKVEELGHDRFTLPVLSYQVHTPEPSISHVPSIPLPSQEHSLP
                     EPHQASQGPAAAHYEHTVVHDHHDTSQANSSSSHQASNSHQTNHTHDHHVSMDM"
     gene            745..1593
                     /locus_tag="PKZ53_19940"
                     /pseudo
     CDS             745..1593
                     /locus_tag="PKZ53_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          470 a          455 c          473 g          417 t
ORIGIN      
        1 cacccgaagg tggaggagtt aggacacgac cgcttcactt taccggtgct ttcgtaccag
       61 gtgcataccc cggaaccttc catttctcat gtccccagca ttcctttgcc aagtcaggaa
      121 cattccctgc ccgaacccca ccaagcgagt cagggaccag ccgccgcgca ttatgaacac
      181 actgttgttc acgatcacca tgacaccagc caagcaaact ccagttcgtc gcaccaagcc
      241 tcaaactcgc atcaaaccaa ccatacccat gatcatcatg tgtcaatgga tatgtagggc
      301 tacggcacct cctgaatact cacgtccttc gacccctatc ccacaccggg aaacctggat
      361 gctggtagct tcccagcaaa tgaaaacagg agactcgaac aggcaaggtc ctattccaaa
      421 atccgcacgg gttgttgatg gatccggtaa aattggttgc tgtcgcacac ttaccatggc
      481 tggttttcgg aaagtggtcc tgaatttgaa aatccttcga atcacggttt gggggctggt
      541 cttttaacca ttccttccag gcgctcagca gaaaactgct cttggggaat accgaccata
      601 cctgaatgtt gggatggctc attttcttaa tgtctcagga gggttggaaa tcctttatcc
      661 agacccgagg ccactaagaa actatctcaa aacgaaaaaa ttaggtaaac catctggaac
      721 acatatgttg ataaggagaa ccagatggat accgatgtcc aacagaataa gccactgccg
      781 acgatttggc gagtttcaga tgccttgtgg gcagtaattg aacccgtatt gcaaaggctt
      841 gatccaccta caaccaccgg acgcaaacga atcgaggcgc gtgcggcatt ggaggggata
      901 attttccggt tgcggagcgg atgtcaatgg aatcacttgc ccaaagagta tccggatgac
      961 agttcgatcc accgaaccct gcaccgctgg gtcaagaaca aagtgtttga agcgatctgg
     1021 gcaattttga ttgagcattg tgctgaatga ggcggtgtgg attgggaatg gcaatccgcg
     1081 gatgcggcgc tgcataaggc ccggttcggt ggtgaccagg tcggtcgcaa tccgactgac
     1141 cggggaaaag ccgggagcaa acgcagcctg ctggtcgaag ccaatggtgg gccgttggcg
     1201 gtggtggtgg cggctgccaa tgttcacgat accaaactcc tggacgctac gatcagggca
     1261 attgtggtcg agcgcccgct ggtgaccgcc gatgaaccac agcatttgtg tctcgataag
     1321 ggcgatgaca atccaaccgg acacgccacc gtggcccaac atgactatct tccgcatatt
     1381 cgccggattg gagaagaaaa attcgatgac acccatcaga aacgctatcc agcacgccgg
     1441 tgggtggtgg aacgaacttt gagctggtta tccaaatgtc gggcattatt ggcgcgctat
     1501 gacaagcgtt catcgaatta tctggctttg gttcaactcg cctgtgcttt actctggttt
     1561 cgcaggtgtc actctttaaa cgttttgaga tagtttctaa ctctacaaca ccgttttggg
     1621 agtttgtgcc gggctctgga atttttatct cagagactgt ttggaaattc caggtacgac
     1681 ctggtttcaa tcggtaagag ggggaaccaa aaggtcaacc caacaactgg aaccagtgaa
     1741 taggttgaat tcaccccagc gatgtgatga ggtgaaatgg aaataaggtg agggtcagtt
     1801 caaaataagg gaatg
//
LOCUS       DAOQDO010001479         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67632_length_1813_cov_2.621729, whole genome shotgun sequence.
ACCESSION   DAOQDO010001479 DAOQDO010000000
VERSION     DAOQDO010001479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67632_length_1813_cov_2.621729"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..895)
                     /locus_tag="PKZ53_19945"
     CDS             complement(29..895)
                     /locus_tag="PKZ53_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42770.1"
                     /translation="MKIKSQSLPTRCEICHQLDEFDPVLNHCQRCCTINYQVAALPEP
                     VPEPAPETPGVTRLRKVLSGLKISFYLSVFGTFFGLPLVGKMLPPLSFDLDWIGVFQQ
                     RLFLVTMLALLSIGFVKIMIFIRADKTSLKAREVEWFVAGCIVAVLFCIMFPGPISAR
                     RSANRQVILQALTNIHRAQEAYRSGPGKGQYAPTLAWLTSQIQPEKALTEREAALETI
                     GLAGYQLKSFQVFPATSWSPARYELKVAPQIRTGMFRGGNHQYYLDQSGVIRESGDSK
                     KPANAQSPREVS"
     gene            complement(969..>1813)
                     /locus_tag="PKZ53_19950"
     CDS             complement(969..>1813)
                     /locus_tag="PKZ53_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42771.1"
                     /translation="SLPTRCEICHQLDEFDPVLNLCRRCQALNPYVSKLHDGITQAKR
                     RRMYWSWIPRVKIGIGICYVLIILGILSGLTNIDSTLVKLPIDPDWLTMVRICMGITG
                     TSLLISFGILSLLTCYWIDWQFRFVSLPDLIILGVVFWVFGSIGLPGAIAAHRSANTY
                     QAIQIVEKIRTDQDSFLKTHGYYASGVDQLVQSSDCPNGLTAMEARVGRIGYHGYFLA
                     SVHTIPATNTLPARYEIQVVPTIRTGWLRSGNDAYFLDQTGNIRHSRSPTKLADVNSD
                     LFSY"
BASE COUNT          495 a          429 c          425 g          464 t
ORIGIN      
        1 attggaagcg attcagattt gattttcatt agcttacctc tcgtgggctt tgagcgttgg
       61 ctggcttttt tgagtctcca gattcgcgaa tcaccccgct ctggtccagg tagtattgat
      121 gatttccgcc ccgaaacatc ccggttcgaa tttgtggagc aaccttgagt tcatatcggg
      181 cggggctcca ggatgtggct ggaaatactt gaaatgattt gagttgatac ccggcaagcc
      241 caatcgtttc aagcgcggct tcacgttcag tcaacgcctt ctcaggttga atttgtgaag
      301 tcaaccaggc caaagtcgga gcgtactgcc cttttccggg cccgcttcga taggcttcct
      361 gggcgcggtg aatgtttgtt aatgcctgaa ggattacctg tcggtttgcc gaacgccggg
      421 ctgagattgg ccccggaaac atgatgcaaa acagaacagc cacaatacat ccggcaacaa
      481 accattcaac ttctcgtgct ttgagagatg ttttgtcagc ccgaataaaa atcattattt
      541 tcacaaagcc aattgagagt aaggccagca tcgtcactaa aaacaaacgt tgttgaaaca
      601 cgccaatcca gtccaaatca aatgacaacg gtggaagcat tttcccaacc aacggcaacc
      661 caaaaaacgt cccaaacacg gacaaataaa agctgatttt caacccggag agtacctttc
      721 gaagtcgcgt tacccccgga gtttcagggg ctggctcggg gactggctcg ggtaaagctg
      781 caacctggta gtttatggtg caacatcgct ggcaatgatt caaaaccggg tcaaattcat
      841 ctaattgatg acaaatttca catctggttg ggagtgattg agacttgatc ttcatgaggt
      901 tccctcacat caatatggct accagatcat aaccagttga atactgacca gttgctggct
      961 ggcacatctt agtaggaaaa cagatcgctg ttgacatcgg ccagtttagt tggactcctc
     1021 gaatggcgta tgtttccggt ctggtccagg aaataggcat cattcccgct cctgagccag
     1081 ccagttcgaa tggttggcac cacctggatt tcatatcgag caggaagggt atttgtggct
     1141 ggaatcgtgt gaactgaggc gaggaaatag ccgtgatacc cgatccttcc aactctggct
     1201 tccatcgcag tcaaaccgtt gggacaatct gaggattgta cgagttggtc aacaccagaa
     1261 gcataatatc cgtgagtttt caaaaatgaa tcttgatcgg ttctgatttt ttcaacgatc
     1321 tggattgcct gataggtgtt ggcacttcgg tgggcggcaa tcgctcctgg taagccaata
     1381 gaaccaaaaa cccaaaaaac aaccccaaga atgatcaaat ctgggaggga aacaaatcga
     1441 aattgccagt cgatccaata gcaggtcagg agtgataaaa tcccaaaact gatgagcaga
     1501 gaagtccctg taatccccat acaaatccgc accatagtta gccaatcagg gtcaattggc
     1561 aatttcacca gtgtggaatc aatgttggtg agtccgctga gaattcctaa aatgatcaac
     1621 acgtagcaga tacctatccc aatttttacc cgtgggatcc atgaccagta catccgtctt
     1681 cgctttgctt gtgtaatacc atcgtgtaac ttgctaacat atggattcaa tgcctgacat
     1741 ctccggcaca ggttcaacac cgggtcaaat tcatctaatt gatgacaaat ttcacatctg
     1801 gttgggagtg att
//
LOCUS       DAOQDO010001480         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67686_length_1812_cov_3.109277, whole genome shotgun sequence.
ACCESSION   DAOQDO010001480 DAOQDO010000000
VERSION     DAOQDO010001480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67686_length_1812_cov_3.109277"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1024
                     /locus_tag="PKZ53_19955"
     CDS             260..1024
                     /locus_tag="PKZ53_19955"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNJ42772.1"
                     /translation="MTDRINYWDYIKLDELHRLQSNIQTDKYDEVMFIAIHQHFELWF
                     FQIIRNLDEVIRRLQASPPEVVSATALMNRVNQEFICATQGFEVMKTLSRESFLSFRD
                     ALRGASGLQSAQLTIIELLTGRHGDEMYHELVSGSGMQDFLAKYRGPDGILTRIAQER
                     LGKGTLRTAYDHVASFPDVQPEDLEPLKVQIARFDEQLFGWRKLHYEAAATVLGPEFT
                     STSGTVGNQHSCSENLKTGMEHTRRYFDDLAHSAKS"
     gene            1110..>1812
                     /locus_tag="PKZ53_19960"
     CDS             1110..>1812
                     /locus_tag="PKZ53_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42773.1"
                     /translation="MICITLTGWVDPFSTCCAQSEFQTESPTRANVAPYSAVAAPEWD
                     ALFDRQTGWTGADGIYSIPISGDERPGTAGNTTTFWVFSDTFIGSVNTQNQRVEGSTI
                     IRNTSALLTGGLPNPNQIQFLWKTSTTGAPLPRVVPTTSAAHWFWPNDGLVVNGRLYL
                     FSLRMKPGGSGVFNFATDGISLLSDSVANLADPFRTYTQVDTRLFQAASGAQGELAFG
                     MAVMPNTTTAGAPRPD"
BASE COUNT          429 a          497 c          484 g          402 t
ORIGIN      
        1 aagacagcgg gctcagggct gaagacagcg ggctcagggc tgaagacagc gggctcaggg
       61 ctgaagacag cgggcttggg gcaatcgaag ggatgaggga tgaaggatga gggatgaaat
      121 aaaaccagct cttcagccca atgtcttcag ccctcagccc caagcccgac ttcttcagcc
      181 ctcagcccca agcccgacgt cttcagcccg ctgccgtcag ccccaaaggc catcacattc
      241 cccaagcgag aacagataca tgactgaccg aatcaattat tgggattaca tcaaacttga
      301 tgaacttcat cgccttcaat caaatatcca gactgacaag tatgatgagg tgatgtttat
      361 tgccatccac cagcattttg aactctggtt tttccaaatc attcgcaatt tggacgaagt
      421 gatccgccgg ttgcaggctt cccccccgga agttgtctca gcaacggccc tgatgaaccg
      481 ggtcaatcaa gaatttatct gtgccactca gggttttgaa gtgatgaaga cgctctcgcg
      541 ggagagtttt ctttcgtttc gtgatgcctt gcgcggggct tcgggcctgc aaagcgccca
      601 gttgactatc atcgaactgc tgaccgggcg ccacggcgac gaaatgtatc acgaactggt
      661 ctctggaagc ggcatgcagg attttctggc caaatatcga ggaccggatg gtattttgac
      721 gcgcattgcc caggaacggc tgggcaaagg aacgcttcga actgcctatg accacgtcgc
      781 cagcttccct gatgtccagc ccgaagattt ggaaccgctc aaagttcaaa ttgcccggtt
      841 tgatgaacaa ctcttcggct ggcggaaact gcattatgaa gcggcggcga cggtgctcgg
      901 accagaattc acttcgacat cgggcacggt tggcaatcaa cattcctgtt ccgaaaacct
      961 gaaaaccgga atggaacata cccgccgcta cttcgatgat ttggcccaca gtgccaaaag
     1021 ctaacccaac gtcaattcaa cccaaacttt tttgaatgcg gggtgagaaa atgctcacaa
     1081 agcagttggc tttccgtctt ctggcgctga tgatctgtat tactctcact ggctgggtgg
     1141 atccattcag cacctgttgt gctcagtcag aatttcaaac cgaatcgcca accagagcaa
     1201 atgtggcgcc ctatagtgcc gtggcggctc cggaatggga cgcgctattt gatcggcaaa
     1261 ccggctggac tggcgcggat gggatttatt cgattccaat ttcgggtgac gagcgtcccg
     1321 gcacggctgg caataccacg acattttggg tttttagcga cacgtttatt ggttcggtca
     1381 acacgcaaaa ccagcgagtc gaaggcagca ccatcattcg caacaccagt gccctgctca
     1441 cgggcgggct gcccaatcca aatcaaatcc aatttttgtg gaagacttca accacgggcg
     1501 ccccgctgcc gagagtggtg ccaacaacgt cagccgcaca ctggttctgg cccaatgatg
     1561 gactggtcgt caatggcagg ctgtatctgt tcagcctgcg catgaaaccc ggtggcagtg
     1621 gcgtgtttaa ttttgcaacg gatggcattt ccctgctcag tgattcggtg gccaatctgg
     1681 cggatccatt tcgcacctac acccaggtag atacccggtt gtttcaagcc gcaagcggtg
     1741 cccagggcga actggctttt ggaatggcgg tgatgcccaa caccacgacg gcgggtgccc
     1801 cacgtccaga tg
//
LOCUS       DAOQDO010001481         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67691_length_1812_cov_2.867957, whole genome shotgun sequence.
ACCESSION   DAOQDO010001481 DAOQDO010000000
VERSION     DAOQDO010001481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67691_length_1812_cov_2.867957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..769
                     /locus_tag="PKZ53_19965"
     CDS             113..769
                     /locus_tag="PKZ53_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42774.1"
                     /translation="MPIPAADRTGLISQSPVGGEEILLTDTFEIRWTSTDNIGVVSHD
                     ILLSLDSGATFNETIVTGLPATTLSFQWTPAEYLEPTTQARIAVRAIDAGFNFGTASS
                     PADFSLIAGVDTEPPSVRVIYPNGEEKLKSKSSTTITWLSSDASDLRSHEIFLSTDGG
                     QTFPTKLGEASGTANSFIFEIPKSLTTKKGMIRVVATDMAGNIGQDESNGVFIIKKKK
                     "
     gene            1110..>1812
                     /locus_tag="PKZ53_19970"
     CDS             1110..>1812
                     /locus_tag="PKZ53_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42775.1"
                     /translation="MKQSVTQHWPVWVAVAILWAIVGGIWVSFTRQTSAIGPIYILDD
                     AYIHMAMARHFSQSFVFGVTPYAFSSSSSSLLWTVLLALGYRIFGVSQSLPLVFNLFI
                     ATGLLVLIASIYYRSFTNQWGLFFLLIGTVVWSPMVAIIFSGMEHLLQALVTIGLLFE
                     LAAHATSTQPRPTSRLLWLAPLAILVRFESCALILPGLLVFTRNKQWKTVIQLGGLAL
                     LPIVIYAGISLSLGWL"
BASE COUNT          405 a          487 c          435 g          485 t
ORIGIN      
        1 gccattcgcc atgacatagg tcgctcccac caggcgagct tcgtcggcga cacgccgctg
       61 atagtaaaac gactcgcccg ccaccgcgca ctcccctgcc gcgcggcggc ggatgcccat
      121 tccggcggcg gaccggaccg gtttgatcag ccagtctccg gttggcggcg aagaaatcct
      181 gttgaccgat acttttgaaa tccgctggac atccaccgac aatatcgggg tggtcagcca
      241 cgatattctg ctttcgctcg atagcggtgc cacgtttaat gagacaattg tcaccgggct
      301 tcctgccacc acgctctcgt ttcagtggac accggctgag tatcttgagc caaccactca
      361 ggcccggatt gcggttcggg ccattgatgc cgggttcaat ttcggaaccg cctccagccc
      421 ggctgatttt tccttgattg ccggagttga taccgaacca ccatcagtac gagtgattta
      481 tcccaacggt gaggagaaac taaaatccaa gagcagcacc accattacct ggctttcatc
      541 cgacgcaagc gatcttcgaa gtcatgaaat tttcctttca acggacggcg ggcaaacctt
      601 cccaaccaaa ctcggtgagg cttcaggcac ggcgaattca tttatttttg aaattccaaa
      661 atcactgacg accaaaaagg ggatgattcg agttgtggca acggatatgg ctggaaacat
      721 cggccaggac gaaagtaacg gagtttttat aattaagaaa aagaaataaa ttctgatctc
      781 acgccctgaa gccgtcaacc aaggttcagt tggatgaatg agcaaacgcc ctctggcata
      841 tggcatttcg gacaataatt ccggttcaga ataacgcctt caggcgtgag acggaacgag
      901 ttggacgagg gataaaacct ctcgtctaaa gacaagactc tcaacctttt ttgaagagct
      961 acatatccca gaaaccattg aattatttga atttactctt cgtgtacttt gtgtatttcg
     1021 tggtttcttt atctgaaatt ccagagtggt tcacttgacc accttagcga aacattttca
     1081 tgaaagtgcg gtctcatctt cttctgtggt tgaaacagag cgtcactcaa cactggccag
     1141 tgtgggtagc cgtggcaatt ttatgggcaa tcgtgggtgg gatctgggtc agctttacca
     1201 ggcagacatc ggccataggg cccatttata tcctggatga tgcctatatt cacatggcaa
     1261 tggccaggca tttttctcaa tcctttgtct tcggagtgac accctacgct ttttcctcta
     1321 gctcctcttc actcctctgg actgtgctcc tcgcccttgg ctatcgcatc tttggtgtga
     1381 gtcaatcact ccccctggtt tttaatcttt tcatagcaac tggcctgctg gttctcatcg
     1441 cttcgattta ttaccgctct ttcacaaacc agtgggggct gttttttctc ttgataggca
     1501 ccgtggtatg gtcgccaatg gtcgccatca ttttttcagg gatggagcat ttactccagg
     1561 cactggttac gattggattg ttgtttgagc ttgccgccca cgccacgtca acccagcctc
     1621 gaccgacgag ccggttgcta tggttggccc ccctcgcgat cctggttcga tttgaaagct
     1681 gtgccctgat tttacctggg cttctggtgt tcacacgaaa caaacagtgg aaaacagtca
     1741 ttcaactcgg agggctggca ctcctgccga ttgtcattta tgccgggatc tcgctctccc
     1801 ttggctggct tc
//
LOCUS       DAOQDO010001482         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67750_length_1811_cov_3.353075, whole genome shotgun sequence.
ACCESSION   DAOQDO010001482 DAOQDO010000000
VERSION     DAOQDO010001482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67750_length_1811_cov_3.353075"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1092)
                     /locus_tag="PKZ53_19975"
     CDS             complement(4..1092)
                     /locus_tag="PKZ53_19975"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ42776.1"
                     /translation="MFEYFPENELEFLRECELYANLSERTLQSIFKRGRLVQLSPGQE
                     LFQVGAPADNFYVVKSGMVEICRFGDKPEEIVTVAFLGRGDSIGEITMLTGTPHAVLA
                     RAPEGGEVFEVPRRNFRTMLEAYPEFAIHLCNTFAERLESSVKNESGTRNRQFHGHLK
                     YFDLATVIQTIVVSRMTGRLVVTDDFGNAYAEIYFDAGDPSCAKIGHLSGPEAFNQLF
                     QPPPVEGTFDFKSGERPPGELLGLFEGCPPGMNMLMDAIRMQDELDNFRPQIDLESIY
                     QPQQTELIWEGEERYLVLANDIWYRLHAEQPTVVQLLQEVPSCHYGVYYVLSTMKQAG
                     LIVSSMDTGLWQKVDSAEGWGLLDDDEV"
     gene            1360..1764
                     /locus_tag="PKZ53_19980"
     CDS             1360..1764
                     /locus_tag="PKZ53_19980"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNJ42777.1"
                     /translation="MNETPSAVRPSPKAPAIRTMMMPHQTNAFGTIFGGVILSYIDLA
                     GAVPARELASHQYVTVAMKEVIFVEPVYVGDLVSFYAEVSRVGRTSVTVHVVVESERW
                     REPGKVVKVTEADVIYVAVGDDGRPTPIQRKE"
BASE COUNT          470 a          449 c          461 g          431 t
ORIGIN      
        1 ggattaaact tcgtcgtcat caagtaaacc ccagccttcg gcggaatcca ctttctgcca
       61 taacccggtg tccattgatg agacgatcaa cccggcctgt ttcatcgtgg ataacacata
      121 atagacgcca taatggcagg atgggacttc ctgcaagagc tggacgacgg tcggttgttc
      181 ggcgtgcagg cgataccaga tgtcattggc caggaccaga tagcgttctt ccccttccca
      241 gatcagttca gtttgttggg gttgataaat tgattcaagg tcaatttgcg ggcggaaatt
      301 atcaagttcg tcctgcatcc ggatagcgtc catgagcatg ttcatgcctg gcgggcagcc
      361 ttcaaacaac cccagcaatt cacctggcgg gcgctcacca ctcttgaagt caaaggtgcc
      421 ttcgaccgga ggcggctgaa aaagctggtt aaaggcttca gggccagata aatgaccgat
      481 tttggcacag gacggatccc cggcatcgaa ataaatttcg gcataggcat tgccgaaatc
      541 atcggtcacg accagccggc ctgtcatccg tgacaccaca attgtttgaa tgaccgtggc
      601 caggtcaaaa tattttaaat gcccgtggaa ctgccggttc cgggtaccac tttcattttt
      661 gacagaagat tccagccgtt cggcaaaggt attgcagagg tgaatggcaa actcagggta
      721 ggcttcaagc atggtgcgga aattgcggcg tggaacttca aacacctcac cgccttcggg
      781 tgcgcgggcg agcaccgcgt gaggagtgcc ggtcaacatc gtaatttcac caatcgaatc
      841 acctcgcccc agaaatgcaa ccgtcacgat ctcttctggt ttgtcaccaa accggcaaat
      901 ttccaccata cctgacttta cgacatagaa gttatcggct ggtgccccga cctgaaacag
      961 ttcttgccca ggtgagagct gaaccagtcg gccacgttta aagatagact ggagcgtcct
     1021 ttccgaaagg ttggcataga gctcacactc acgcaaaaac tcaagttcat tttcgggaaa
     1081 gtactcgaac atgggacact tcctgtgaaa gggctgggct ggagatgcaa ggttgagatc
     1141 ggagtggaat tcctattgtg tcaaaagggc gcgccatggt caaatcaccc gctgcatttc
     1201 agttgactga aatgagacaa agtgcgcgtc attttctcgg agtcgtaaaa agttaacttg
     1261 ttgaaaaaat tgtatttccc tgacccatac tgccccaacc ctgaaccctg aacggcattg
     1321 cacaacccac aacagaattt ttacacacat aaaatactta tgaatgaaac tccatccgct
     1381 gtccgcccat cgccaaaagc gcctgccatc cgaaccatga tgatgcccca tcaaaccaat
     1441 gcctttggca cgatctttgg tggtgtcatt ttaagttata tcgatctggc cggggcggta
     1501 ccggcacgtg aactggcctc tcatcaatat gtaactgtcg cgatgaaaga ggtcattttt
     1561 gtcgaaccgg tctatgtcgg tgatttggtg agcttttacg ctgaagtctc gcgggtcggg
     1621 cgaacgtcgg tgaccgttca tgtcgtggtt gagtctgaac gctggcgtga acctggtaag
     1681 gttgtcaaag tcactgaagc cgatgtgatt tatgtcgccg ttggtgatga tggtcgccca
     1741 accccaatcc agcggaaaga atgaagaatg aagaattgag aatgaagaat tgagaatgaa
     1801 gaatgaagaa t
//
LOCUS       DAOQDO010001483         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67753_length_1811_cov_3.101367, whole genome shotgun sequence.
ACCESSION   DAOQDO010001483 DAOQDO010000000
VERSION     DAOQDO010001483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67753_length_1811_cov_3.101367"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PKZ53_19985"
     CDS             <1..918
                     /locus_tag="PKZ53_19985"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNJ42778.1"
                     /translation="GNANRPLAEEICRHLGIPLGEANTSRFSDGEFNYQINENVRGAD
                     VFLVQPTCPPVDASVMELLIMIDAFRRSSADRITAVIPYYGYARKDRKDRPRVPISAK
                     LVANLITKAGADRVLTMDLHAGQIQGFFDIPVDHIFAAPVILEYLANKNIPDLVVVAP
                     DAGGVERARAYAKRLGVDLALADKRREKERANFVEVMNIVGNVNGRNVVIVDDMVDTA
                     GSLTQVAGALREKGAQGIYACCTHAVLSGPAMDRIAASPIKEVVVTNSIPLRVVDEAQ
                     KIKPLSVAKLLADAIKSIHNSTSVSKLFI"
     gene            972..1538
                     /locus_tag="PKZ53_19990"
     CDS             972..1538
                     /locus_tag="PKZ53_19990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNJ42779.1"
                     /translation="MISDVTLSAEPRTKTGSTESRRMRREGVVPVSVYSGGQDAVSVQ
                     VNARQLAALLRSEEKRSSIFYLEIAGKEKTPVRIKNLQLHPVRGNVLHADFLWVDLAV
                     SLKVKVPIRLVGDAEGVKLGGTLDHGVREITVECLPADIPAHIDVTVTKLKIGDQLLA
                     SDVVLPSGVRLVSDSHQKIAVVSAARAQ"
     gene            complement(1499..>1811)
                     /locus_tag="PKZ53_19995"
     CDS             complement(1499..>1811)
                     /locus_tag="PKZ53_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42780.1"
                     /translation="VSYFMPLQHLTPAEAVPILQQAVPPHPYGVYVPVPNAQAIIITE
                     NSSVIRQLVALQELIDVPPTKMISQFVTSPGFPNPTRIFTAYFKLRNYCARAALTTAI
                     F"
BASE COUNT          442 a          416 c          506 g          447 t
ORIGIN      
        1 ggaaacgcaa atcgaccatt agctgaagaa atttgccggc acctgggcat tccgttgggc
       61 gaagccaata cctcacggtt cagtgacgga gagtttaact atcagattaa tgaaaacgtg
      121 cgcggagctg acgttttcct ggttcagcca acctgtccgc cagtcgatgc cagcgtgatg
      181 gaattgctga ttatgattga tgcattccgt cggtcgtcgg ctgatcgaat tacggctgtg
      241 attccctatt acggctatgc ccgaaaagat cgcaaagacc gtccacgcgt gccaatttca
      301 gccaaactgg tcgcgaactt gatcaccaaa gccggagctg atcgggtttt gaccatggat
      361 ctgcatgctg gccagattca ggggtttttt gatattccag tcgatcatat ttttgcggca
      421 ccggtgattt tagagtacct ggccaacaag aatattccag acctggttgt ggtggccccg
      481 gacgctggcg gagttgaacg ggctcgtgcc tatgccaaac ggttaggtgt tgatctggcc
      541 ctggctgaca agcgtcgtga aaaagaacgc gccaattttg ttgaagtgat gaacattgtt
      601 ggcaacgtca atggtcggaa tgtggtgatt gtggacgata tggttgatac ggctggatcc
      661 ctgacccagg tggctggagc cctccgggaa aaaggcgccc agggcattta tgcctgctgt
      721 acacatgcgg tgttgtctgg accagcgatg gaccggattg ccgcatcacc gattaaggaa
      781 gtagtggtta ccaactctat ccccctgcgg gtggtggatg aggcacaaaa aattaaaccg
      841 ctgagtgttg ccaagttgct ggctgacgcc attaaatcga ttcataactc aacttcggtc
      901 agcaaattat ttatctaaac gacctgttgg acttcgccgg agacaggccg catgtaagga
      961 gtagttttgt catgatttct gatgtcacgc tgagtgctga acctcgaacc aaaaccggct
     1021 cgaccgagtc ccgccgcatg cggcgcgaag gggttgtccc ggtaagtgtt tactctggtg
     1081 gtcaggacgc ggtttccgtc caggtcaatg cccgtcagtt ggcggcattg ctgcgttcgg
     1141 aagaaaaacg aagctcaatt ttttaccttg agattgctgg aaaagaaaaa acacccgtcc
     1201 gaatcaaaaa cttacaattg cacccggttc gcggaaacgt cctccatgcc gattttctgt
     1261 gggtggatct ggccgtcagc ctgaaggtaa aagtgccgat tcggctggtt ggcgatgctg
     1321 aaggggtcaa acttgggggc accctcgatc acggcgtgcg cgaaatcacc gtggaatgtt
     1381 tgccggctga tattccagcc cacattgatg tcacggtgac caaactcaag attggtgacc
     1441 agttgctggc cagtgatgtc gtgctcccaa gcggagtccg cctggtgagt gatagtcatc
     1501 aaaagatagc cgtggtcagc gctgcccgtg cgcaataatt ccttaacttg aaataagctg
     1561 tgaaaattct ggttgggtta ggaaatccag gagacgtgac aaattgtgaa atcatctttg
     1621 tcggcggcac atcgattaac tcctgtaaag ctactaattg acgaatcacg gaagaatttt
     1681 cggtaatgat aatagcctga gcattcggta caggaacgta tactccataa gggtgaggtg
     1741 gtaccgcttg ttgtaagatc ggaactgctt ctgcaggggt aagatgttgt aaaggcataa
     1801 agtagctaac g
//
LOCUS       DAOQDO010001484         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67817_length_1810_cov_3.479772, whole genome shotgun sequence.
ACCESSION   DAOQDO010001484 DAOQDO010000000
VERSION     DAOQDO010001484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67817_length_1810_cov_3.479772"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1810)
                     /locus_tag="PKZ53_20000"
     CDS             complement(<1..>1810)
                     /locus_tag="PKZ53_20000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ42781.1"
                     /translation="LTAWQIFLARMTGTKLVTIGVMDDGRTVDGLAHTFGLLARPVPV
                     CSELIEHTSFASAVKHTLDAVSETTEWQEYFDFEALFPASSQARIHFQFEFLDFRPET
                     PTTTEQFTLYRLRSIPDPFHLKLIGIHYPDKFSLEIQFDRAQIEHRYAAHLARCFETL
                     LTSLVETPESEVGKLNLLSDEDRQFLLVQLNQTAREYPTISCLHELFENQVRRIPDEP
                     ALVYQDANQSDTEDEFNDFDEFDDFDDFGEDDETASHLQILTFREVNARADRLARHLQ
                     SFGIGPEMLVGICSTHRPETIIGMLGILKAGAAFVPLDPTYPKERLAFLLSDSNVRVL
                     LTETRFKDRLPDFDGTIVYLDEPFNPQRRILQPSGVTPENLAYVIYTSGSTGQPKGVM
                     IEHRSVVNYLHWCLENYPTNFPHGAPLHSPIGFDLTITSLFAPLLAGRSVWITTEDVG
                     VSALAKALTDAKGFGFVKLTPTHLGMLNQELANAPISKKVGALIIGGEALSGETLTFW
                     QTHSPTTRLINEYGPTEATVGCCVYEVPPGQTISGPVPIGKPIANMQMFVLDAQGEPV
                     PFGVPGEIFIGGIGLARGYVGDRGQRMEDSAEKPGFR"
BASE COUNT          417 a          450 c          516 g          427 t
ORIGIN      
        1 cccggaaccc tggtttttct gcactgtcct ccatcctctg tcccctatcc cctacataac
       61 cacgtgccag cccaatgccg ccaatgaaga tttcacccgg aacgccaaat ggaaccggtt
      121 cgccctgggc atccaggaca aacatctgca tattggcaat tggtttcccg atagggaccg
      181 ggccggaaat agtctggccg ggtggaactt catagacgca acagcccaca gtggcttcag
      241 ttgggccata ttcgttgatc aaccgcgtgg ttggcgagtg tgtttgccag aaggtcaggg
      301 tttccccgga aagtgcttcg ccgccgatga tcagggcgcc gacttttttg gaaatagggg
      361 cgtttgccag ttcctggttg agcattccca gatgagttgg ggtcagtttc acaaacccaa
      421 atcctttggc atcggtcagg gctttggcga gtgcggaaac gccgacatct tcggttgtaa
      481 tccagacgct tcgcccggcc agaagtggag caaacaggct cgtaatggtc aaatcaaacc
      541 caattgggga atgcagcggc gccccatgcg ggaaattggt cggatagttt tccagacacc
      601 agtgcaggta attgacgaca ctccggtgtt caatcatcac gcctttgggc tgtccggttg
      661 agcctgaggt gtaaatcaca taggccaggt tctctggggt cacgccagac ggttgcagaa
      721 tgcggcgttg cggattgaat ggctcatcca ggtagacaat tgtcccgtca aagtcaggta
      781 accggtcttt gaatctggtc tcagtgagca gcacgcgcac gttggaatca ctgagcagaa
      841 aggcaagtcg ttctttggga taggttgggt caagcggtac aaacgccgct ccagccttta
      901 aaatcccaag catcccgatg atggtttccg gacgatgggt gctgcagatt ccaaccagca
      961 tctccgggcc gataccaaac gattgcagat ggcgtgccag acggtcagcc cgggcgttca
     1021 cttcacgaaa ggtcaggatt tggaggtgtg acgctgtttc atcgtcttca ccaaagtcat
     1081 caaagtcatc gaactcatca aagtcattga attcatcttc ggtgtctgac tggttggcgt
     1141 cctgatatac cagggcgggt tcatcgggga tgcgtcgaac ctggttttca aacagttcgt
     1201 gaaggcaact gatggttggg tattcacgcg ctgtttggtt cagttggacc aggagaaatt
     1261 gacgatcttc gtcgctcaac aaattgagtt tcccgacctc tgattccggt gtttcgacca
     1321 gactggtgag caacgtttca aagcagcggg ccagatgggc ggcatagcga tgttcaatct
     1381 gagcccggtc aaactggatt tcgagcgaaa atttgtctgg gtaatgaatg ccgatcagtt
     1441 tcaggtgaaa cgggtccggg attgatcgca ggcgatagag cgtgaattgc tcagtggtgg
     1501 ttggggtttc gggccgaaaa tcaaggaatt caaactggaa atgaattcgt gcctgggaag
     1561 aggcagggaa cagggcttca aaatcaaagt attcctgcca ttccgtcgtt tctgaaacgg
     1621 catcaagcgt gtgtttaacg gcgctggcaa agcttgtatg ttcaatcagt tcagaacaga
     1681 ccggcacggg tcgcgccagc aagccaaaag tatgtgccag tccgtcaaca gtgcgtccgt
     1741 cgtccatcac gccgatggtg accagtttgg tgccggtcat tcgggccagg aaaatctgcc
     1801 aggctgtcag
//
LOCUS       DAOQDO010001485         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67894_length_1809_cov_3.114595, whole genome shotgun sequence.
ACCESSION   DAOQDO010001485 DAOQDO010000000
VERSION     DAOQDO010001485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67894_length_1809_cov_3.114595"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1022
                     /locus_tag="PKZ53_20005"
     CDS             222..1022
                     /locus_tag="PKZ53_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ42782.1"
                     /translation="MAVFTELIRVTNLSFRYGQREILKNISFTVREGEFIGLIGPNGS
                     GKTTLLKLLLGLLPGAEQCVTLGGTPLANLSRKNIALQATLVPQDTKIEFAFSVRDIV
                     AMGRTPYLGRFRPEGPADRAAIHHAMTVTETLDFAERPVTHLSGGERQRVHLARALAQ
                     ETSIILLDEPTSNLDLVHQFEVLELVKKCVANHRSAIAAIHDLSLAARFCNRLLLLFD
                     GMIVADGSPEEVLTEPNLARYFSIEARIRKDPESGSLVVWPVAPCLPL"
     gene            1032..>1809
                     /locus_tag="PKZ53_20010"
     CDS             1032..>1809
                     /locus_tag="PKZ53_20010"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNJ42783.1"
                     /translation="MNLLQKISAQIYPLSPTIKEAAQARHLQLTKPPGSLGHLEQLGV
                     KLAAIQGTVTPSIGKKRIIVCAADHGVTTEGVSAYPRDVTYQMVMNFVRGGAAINVLA
                     RQTGIEIQVVDAGVDFDFPADLPVIHRKIVRGTANFAQGPALTQNEVTACLALGIDLA
                     NQAAAEQVSLLGIGEMGIGNTTAASAITAVLTGIDPALVTGGGTGVEGEVLYRKIGIV
                     RKAIATNQPQPENPVDILAKVGGAEIGVLVGIVLGAAANRI"
BASE COUNT          413 a          488 c          506 g          402 t
ORIGIN      
        1 aaaggtccga tcaagggcga tctggtggtg agttacaccc acccgattgg tgaacaaagc
       61 ctcaaagtct atggcaaggt tgagaacatg tttgatcgaa cctactttga aagcggctat
      121 cgaacaccag gtgcaaccgt tctcggcggc ctggcctttc ggttttaacc atcccagcaa
      181 gagtcagtac cacctgcatg agcgggtggg gttggattca aatggcagta ttcaccgaac
      241 ttatccgggt gacgaacctg agtttccgct acggacagcg tgaaatattg aagaatattt
      301 catttaccgt ccgtgaaggg gaatttattg ggttgatcgg tcccaacggc agcggcaaga
      361 caaccttgct caaactgctg ttgggcctgc ttcccggtgc ggaacaatgt gtcacacttg
      421 gcgggactcc gcttgcgaat ctgtctcgga aaaatattgc cttgcaggcc accctggtgc
      481 cgcaggatac caaaattgag tttgctttta gcgttcgcga cattgttgcg atggggagga
      541 ccccctacct tggccgcttt cgcccggaag gtccggcaga tcgggccgcc atccatcatg
      601 cgatgaccgt aaccgaaacg ctcgattttg cggaacgccc agtcacgcat ctgtcaggtg
      661 gtgaacgcca gcgggttcat ctcgcccgag ccctggccca ggaaaccagc atcattttgc
      721 tggatgaacc aacgtcgaat ctggatctgg tccatcagtt tgaagtgctt gaactggtca
      781 aaaaatgtgt ggccaatcat cgttcggcaa tcgccgcaat ccatgacctg tcactggcag
      841 cccgattttg caaccgactg ttgttgcttt ttgatggcat gatcgtggcc gatgggtcac
      901 ccgaggaagt tctgaccgag ccaaatctgg cccggtactt ttcaatcgag gcccgaattc
      961 gcaaggatcc ggaaagtggc agtctggttg tctggccagt tgcgccttgc cttccactgt
     1021 gagaatccaa cgtgaacctg cttcagaaaa tttcggcaca aatttatccg ctgtcaccca
     1081 caatcaagga agccgcccag gcccgacatc ttcaactgac gaagcctcca ggtagtttgg
     1141 gccaccttga gcagctcggc gtcaaactgg cggccattca gggcacagtc acaccgtcca
     1201 ttggaaaaaa acggattatc gtttgcgccg ctgaccatgg ggtcacaacc gaaggcgtca
     1261 gcgcctatcc ccgtgacgta acctatcaaa tggtgatgaa ttttgtccgg ggtggcgcgg
     1321 cgatcaatgt cctggcccga caaacgggaa ttgaaattca ggtggtggat gccggggtgg
     1381 attttgattt tccagccgac ctgccagtga ttcatcgaaa aatcgtccgg ggcaccgcca
     1441 attttgccca ggggccagct ctcacccaaa atgaagtcac ggcgtgcctg gcactcggaa
     1501 tagatctggc caaccaggca gccgcggaac aggtttcctt gcttggtatc ggagaaatgg
     1561 gaatcggcaa taccaccgcc gccagcgcaa ttaccgctgt gctgaccggg atagacccgg
     1621 cgctggtgac cggtggcggt actggcgttg aaggagaggt cctctaccga aaaattggca
     1681 tcgttcgaaa ggccattgcc accaaccagc cccagcctga aaacccggtt gatattctgg
     1741 ccaaagttgg tggggctgaa atcggagtcc tggtgggaat tgtgctgggg gcggcggcca
     1801 atcgaatcc
//
LOCUS       DAOQDO010001486         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67976_length_1808_cov_2.842556, whole genome shotgun sequence.
ACCESSION   DAOQDO010001486 DAOQDO010000000
VERSION     DAOQDO010001486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67976_length_1808_cov_2.842556"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKZ53_20015"
     CDS             <1..346
                     /locus_tag="PKZ53_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNJ42784.1"
                     /translation="VTPQVSFDAATGIMEIAGESYPENSMQFYQPVFKWLQEFFSSEP
                     GSATFNFKLEYFNTSSSKCILNILEILEDAHSEGHTIELNWYYREDDDDMLESGQEFC
                     EDMTLPFKFISY"
     gene            508..1734
                     /locus_tag="PKZ53_20020"
     CDS             508..1734
                     /locus_tag="PKZ53_20020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42785.1"
                     /translation="MEKEVGRESERSSNVNEVFADELDVVQKAETVLKQESLDPKNLA
                     EEYKFLVKKYRGLLKQAMKITKIGDSTQSKLIRTQQVLDRQNEELGTKNRALQEKNEA
                     LIRAHEALLQAKQRQESIFSALTEALPGTVLDNKYRLEKKIGTGGYGAVFEATHLGLN
                     RPLAVKVLQPRSGLITPNDLERFRREGISACRITHPNAVAVSDFGISTSGIAYLAMEL
                     LQGHTLSDELKEFGRLTAQRSVEILIPVCQALAEAHSAGLVHRDIKPDNIFLHKTPAE
                     EIVKVLDFGLAKLMGEEHRADLSDITQGVIVGTPLYMPPERLSNLPYDGRADIYSLGV
                     VWFEMLTGRVPFIIRKGNLGALVTAHMLQPPPPLRSIVPEISEVLETLVLRTLKKIPN
                     ERPTAKELAEAMTLFL"
BASE COUNT          477 a          447 c          475 g          409 t
ORIGIN      
        1 ggtcacacct caggtctcat ttgatgcggc gaccggtatt atggaaattg cgggggagtc
       61 gtatcctgaa aactcgatgc aattttatca gccagtcttt aaatggctgc aggagttttt
      121 cagttctgaa ccaggatcgg cgacctttaa ttttaagctc gaatatttca ataccagttc
      181 gtcaaagtgc atcctcaaca tccttgagat tcttgaagat gcacacagcg aagggcacac
      241 catcgaattg aactggtact accgcgaaga tgatgacgat atgttggaaa gtggtcagga
      301 gttttgcgaa gacatgaccc ttccgtttaa atttatctct tattaaggag gggtcagtac
      361 cacctgcgtg agcgggtggg ggctcgacac cggcaggccc acccactgac gcgggtggta
      421 ctgactcatc ggttcttctg attcatctcc aacacaaaat ccggtagagg cagcataatt
      481 cataattcca taaacatgag gactggcatg gaaaaagaag ttgggcgtga atctgagcgc
      541 agtagcaatg tgaacgaagt ttttgccgat gaactcgatg tggttcaaaa agcagaaacc
      601 gtgctcaagc aggaatcgct cgacccgaaa aatctggccg aggaatacaa gtttttggtg
      661 aaaaagtatc gtggactgct caaacaggcc atgaaaatca ccaaaatcgg agattcaacc
      721 cagagtaaac tgatccgtac ccaacaggtg ctcgaccgcc agaacgaaga acttggcacc
      781 aaaaatcgag ccctccagga aaaaaacgaa gccctgattc gggctcacga ggcattgctc
      841 caggccaaac aacgccagga aagtattttt tcggccttga ctgaggccct gccaggaacg
      901 gtgctcgaca acaaataccg gctggagaaa aaaatcggca ctgggggcta tggtgcggtg
      961 tttgaagcca cccaccttgg actcaaccga ccactcgccg tcaaagttct gcagccgcgt
     1021 tctggcctga ttacccccaa tgacttagag cgatttcggc gcgaaggaat ttcagcctgt
     1081 cgtattaccc accccaatgc ggttgccgtg tctgactttg gcatctcaac cagcgggatt
     1141 gcctatctgg cgatggaatt gctccagggg cataccctct ctgatgagtt aaaagaattt
     1201 ggccggctca cggcacagcg ttcggttgaa atcctgatcc cggtctgcca ggcgctggcc
     1261 gaagctcact cggctggact ggtccatcgc gatatcaaac ccgataatat ttttctccat
     1321 aaaactcccg ccgaagaaat tgtcaaagtg cttgattttg gtctggccaa gctcatgggt
     1381 gaagaacatc gggctgattt gtctgacatt acccaggggg tgattgtcgg cacaccgctc
     1441 tatatgccgc ccgaacgact cagcaatttg ccctatgatg gccgggcaga tatttacagc
     1501 ctgggtgtgg tgtggtttga aatgctgacg gggcgtgttc cattcatcat ccggaaagga
     1561 aatctgggcg cgctggtgac ggcccatatg ttgcagccgc caccaccatt gcgatcaatt
     1621 gtccccgaaa tttctgaagt gttggaaacc ctggttctcc gaacgctgaa gaaaattcca
     1681 aacgaacggc ccacggccaa agaactggcc gaggcgatga cgttgttttt gtaaaaacca
     1741 gggctgaaga aagcgggctt ggagcggaag actcgcaagc tcggggcgga agaaaacggg
     1801 tttgagac
//
LOCUS       DAOQDO010001487         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_67981_length_1808_cov_2.662293, whole genome shotgun sequence.
ACCESSION   DAOQDO010001487 DAOQDO010000000
VERSION     DAOQDO010001487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67981_length_1808_cov_2.662293"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..617)
                     /locus_tag="PKZ53_20025"
     CDS             complement(36..617)
                     /locus_tag="PKZ53_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNJ42786.1"
                     /translation="MKLRKQLTEDKKLPITPPSGVVDYIALIWATGLLSGYAPVAPGT
                     FGSAVAVIGYYGAAKLGWFSPFSTASVLPLMLVCVVVSYTGIWASNRAEHFFGYKDPN
                     EVVVDEVAGQLITYLFLSLTPNLAKVTWSFEAWTLVGFFVFRGLDILKPYPVRDLERL
                     ESGLGIMGDDILAGIQGAVLMAIAGFIASMMIA"
     gene            complement(804..>1808)
                     /locus_tag="PKZ53_20030"
     CDS             complement(804..>1808)
                     /locus_tag="PKZ53_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNJ42787.1"
                     /translation="ARRMKLDRIVALEEFDVLTAALIREHLRIPGMGSTTARHFRDKL
                     AMRVQARDAGVPVPDFVHVLNYDELAEYMRRVPPPWVMKPRSDVSAIGIKKMHDSEQV
                     WRTIDILDARPALTERSSYYLLEKYVAGDVYHVDSLVENSQVVFAGTNRYGRPPMNVA
                     HDGGVFVTYTLEHDSDDRKALLEENINLIKGLGLVRGATHAEFIKSTEDGKFYFLEIA
                     ARVGGAYIAETLEAATGVNIWREWATIELNRGEHPYTLVPERDEYAGIALSLAKQEFP
                     DTSAYTDPEIIYRVKRSHHVGLIVHSPNYDRVQELLGQYVKRIEHDFSAVHPVPERAE
                     "
BASE COUNT          463 a          501 c          443 g          401 t
ORIGIN      
        1 attcgaaaac ccggaacccg gaacccggaa ccctattatg caatcatcat actggcaata
       61 aaaccggcaa tcgccatcag tacagcaccc tggatccctg ccagaatgtc atcgcccata
      121 atgccaagcc cactttccag tcgctctaaa tctctgactg gatagggttt gagaatatcc
      181 aacccacgaa atacgaaaaa cccaaccagt gtccaggctt cgaaactcca ggtaactttg
      241 gccagattcg gagtcagaga aaggaaaagg taggtaataa gctggccggc gacttcgtca
      301 accacgactt cgttgggatc tttgtatccg aagaagtgct ccgctcgatt gctggcccaa
      361 attccggtat aggaaaccac cacgcacacc agcatcaacg gcaaaaccga agccgtgcta
      421 aagggcgaaa accagccgag tttggcagcc ccataatagc caatcacggc tacggcagag
      481 ccaaacgttc cgggtgcaac cggtgcatat cctgacaaca atccagttgc ccaaattaag
      541 gcaatataat caaccacgcc actcggcgga gtaatcggca actttttgtc ttcggtgagt
      601 tgttttcgta atttcatagt ctttcaggat ggcaaaaggg cgcggcaacc agcagcgccc
      661 ttctttcttt gagttcagct tgtctgatag gtatctcaat ctgcaatgaa atgatcgtat
      721 tagaaaacag tcaagtagtt caatcaaata aacttatcga actactgact acggactaat
      781 aactacaaac tggcaccaga tggtcattca gcccgctctg gaacaggatg aacagccgaa
      841 aaatcgtgtt cgatccgttt gacatactgg ccaagcagct cctgcacccg gtcataattg
      901 ggtgaatgca cgatcagacc aacgtggtgg cttcgcttga ctcgataaat gatttccggg
      961 tcagtgtacg ctgaagtatc cgggaattcc tgtttggcca gtgacagtgc aattccagcg
     1021 tattcatcgc gttcggggac cagggtataa gggtgttcac cccggttcag ctcgatggtc
     1081 gcccattcgc gccagatgtt gacaccggtc gcggcttcga gggtttcggc gatgtaggca
     1141 ccaccgacgc gagccgcaat ttccagaaaa taaaatttcc cgtcttcagt gctcttaata
     1201 aattccgcgt gcgtggcccc gcgaaccagc cctaagcctt tgatcagatt gatgttttct
     1261 tcgagcaaag cttttcggtc gtcagaatca tgttccagcg tgtaggtcac aaagactccg
     1321 ccatcgtggg cgacattcat cggaggacgc ccatagcggt tggtcccggc aaacacaacc
     1381 tggctgtttt cgaccagtga atcaacgtga tagacatcgc cggcgacata tttttcgagc
     1441 aggtaataag atgagcgttc ggtcagggcg ggtcgcgcgt ccagaatatc aatcgtgcgc
     1501 cagacctgtt ctgagtcgtg cattttcttg atgccaatcg ccgagacatc cgaccggggc
     1561 ttcatcaccc acggcggcgg cacccggcgc atatattccg ccaactcatc atagttgagc
     1621 acgtgcacaa aatcgggaac cggcacacca gcatcccgcg cctgcactcg catggccagc
     1681 ttgtcacgga agtggcgggc ggtggtgctg cccattccag gaatccgcag gtgctcgcgg
     1741 atcaatgcgg ctgtcagcac gtcaaattct tcaagcgcca caatccgatc cagtttcatc
     1801 cgacgggc
//
LOCUS       DAOQDO010001488         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68023_length_1807_cov_3.293950, whole genome shotgun sequence.
ACCESSION   DAOQDO010001488 DAOQDO010000000
VERSION     DAOQDO010001488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68023_length_1807_cov_3.293950"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKZ53_20035"
     CDS             <1..625
                     /locus_tag="PKZ53_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNJ42788.1"
                     /translation="ANWELNLQQVTVLSKKLQEMMHQPTLLNDGFPYPYGFGWETDTV
                     NAVARVRHGGSLGGFRTEFMRFPGEKLAVIVLTNQDSANPMKIASLVANTVLPNLKYR
                     PIKDTDPAFSATVKKLLESWADGTVDSSIFTPGCWKVFEPRFKNPNAIKGFKNLGPMT
                     GLSLVESRQTGDFRVTRHRITFASQTQLIQIVRTKAGLITDMRLENE"
     gene            870..1211
                     /locus_tag="PKZ53_20040"
     CDS             870..1211
                     /locus_tag="PKZ53_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HNJ42789.1"
                     /translation="MLIAVAGPYSAPTPKQRQANLDALNRAAAQVYRRGHIPVIGVNL
                     ALPVVEHLDPETDEYEAIMAISLAAIANCDALLLIAESPGANRERDLIAGLGRPVYRS
                     LKELPPASKKS"
     gene            1300..1749
                     /locus_tag="PKZ53_20045"
     CDS             1300..1749
                     /locus_tag="PKZ53_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42790.1"
                     /translation="MASIFTVFAAPDTIHDPLRYVQQVFPETECLELNPLDIDSLSRL
                     ILILDMESGVPRTPLDALTLIESDRVVDDEFEGAEVYRLPVWLPKQLARLSLSRRLEL
                     AVIWWELESPFHRTLELELVEVLVIGLQVISQIAIEDDLKLFLQIQG"
BASE COUNT          407 a          491 c          439 g          470 t
ORIGIN      
        1 ggcgaactgg gaactcaacc ttcagcaagt caccgtcctg tccaaaaaac tccaggaaat
       61 gatgcaccaa ccaactctgc tcaacgatgg cttcccgtat ccgtatgggt ttggctggga
      121 aaccgatacc gtcaatgccg tggctcgcgt ccgacacggt gggtcgctcg gcggcttccg
      181 caccgagttt atgcgctttc caggcgaaaa actggctgtc attgtgctga ccaatcagga
      241 ttcagccaac ccgatgaaaa ttgccagcct cgtggccaac accgtccttc caaatctcaa
      301 gtaccggccc atcaaagata ctgacccggc cttttcagcc acggtcaaaa agttgcttga
      361 atcctgggct gacggcactg tggatagctc gatttttacc ccaggttgct ggaaagtgtt
      421 cgaaccacga ttcaagaacc cgaatgcgat caaggggttc aaaaaccttg gcccgatgac
      481 tggtctttca ctggtcgaat cgcgccaaac cggagatttt cgagtcaccc gtcatcgaat
      541 cacctttgcc agccagaccc aactgatcca gattgtccgc accaaagctg gattgattac
      601 cgatatgcgg ttggagaatg agtagttttc tgagcgagat agtgagatag cgagatagtg
      661 agataccagc ctgatggatt gactcctcgc ttttctcttc gctactcact cctcgctctt
      721 gcttcactct tttcttcgct ccccgctctt ttggcctgtg cagaaaaaaa aattgaaaaa
      781 ggtctgtttt ttctacgcgc tagtcgctat cttggagatc acgtgttttt cgaattcata
      841 cctcagaacc aattctccaa accttactta tgcttattgc tgttgccggt ccttattctg
      901 ctcccactcc gaaacaacgt caggccaacc ttgatgcctt aaaccgggct gccgctcaag
      961 tctatcgccg gggccatatt ccagtgatcg gtgtgaacct ggcactaccc gtggtcgaac
     1021 atcttgaccc ggaaactgat gaatatgaag cgattatggc catctcgctg gccgctattg
     1081 caaactgtga cgccctgtta ctgattgctg aatcacctgg tgccaaccgc gaacgcgacc
     1141 tgattgccgg gttaggccgt ccggtgtacc ggagcctgaa agaacttccc cccgccagta
     1201 aaaaatctta gtttcggacc atctgccact tttttcgtgc accgtcttca ggtttttttt
     1261 cgctttaact gatagagcga atattcctgg agaaaactga tggcgtccat ttttactgtt
     1321 tttgcagccc ccgacacaat ccacgaccca cttcgatatg tgcagcaggt atttcccgaa
     1381 acggaatgcc tggaattaaa cccactggat attgattcac tctcgcggct gattttaatc
     1441 ctggatatgg aatctggcgt gcctcgaaca ccgttagacg ccctgaccct gattgaatca
     1501 gaccgtgtgg tggacgatga attcgaaggt gccgaagtct atcggctccc cgtgtggtta
     1561 cccaaacaac tggccaggtt gtctttgtcg aggcgactcg aactggccgt gatctggtgg
     1621 gaactggaat cgccgtttca tcgaacactc gaactcgaac tggttgaagt cctggtgatt
     1681 ggacttcaag tgatcagcca gattgccatt gaagatgacc tgaaactgtt tttgcagatt
     1741 caggggtgaa tggctggggt tccgggttcc gggttccggg ttccgggttc cgggttccgg
     1801 gttccgg
//
LOCUS       DAOQDO010001489         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68033_length_1807_cov_2.969178, whole genome shotgun sequence.
ACCESSION   DAOQDO010001489 DAOQDO010000000
VERSION     DAOQDO010001489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68033_length_1807_cov_2.969178"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKZ53_20050"
     CDS             <1..1206
                     /locus_tag="PKZ53_20050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42791.1"
                     /translation="HTDITDQKRALDELKKTETQLRHSQRMEAVGRLAGGVAHDFNNL
                     LMVISGYADMVAKKLGKAHALYRFLEEIQQASRSASGVTRQLLAFSRKQDQQLKSLNL
                     TDVVTQLHKLLRRVIGEDIKLVTSLADDTAPVLADPAQIEQVVLNLVVNARDAMPGGG
                     TLTLQTENVVRTTEEQALHFTVPPGNYVSLTVTDTGCGMEEATLERIYEPFFTTKEAH
                     KGTGLGLSTVYGIVQQSNGYILVESQPGTGTRFTVLLPQTPGMDLHQATEEPVVPTDS
                     SNTTILVVEDEDSVRIMVREVLTLWGYRVLEATSGEAAIKQMAHHHDQISLVLTDVVM
                     SGMSGVNLVQHIRENHPAVKVLFMSGYIADASDAVGIASQLDNFISKPFTPLTLVKAI
                     QTVLNESDK"
     gene            complement(1365..>1807)
                     /locus_tag="PKZ53_20055"
     CDS             complement(1365..>1807)
                     /locus_tag="PKZ53_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42792.1"
                     /translation="PRTHEVDQPPEMREYQPVLPAQTATILLVEDEDVVRNLTHHILK
                     DAGFRVYEASSAEAALELIEAQHLQIDFLLTDVVLPGMKGPVLAKRLKEKQPNLPVLY
                     ISGYTENAIAHHGVVDEGVELLQKPFTPKALVQRILAILNAGPK"
BASE COUNT          438 a          457 c          466 g          446 t
ORIGIN      
        1 cacactgata ttacggatca aaaaagagcc ctcgacgaat taaaaaagac tgagacccag
       61 cttcgccatt cccagcgcat ggaggcggtt ggccgactgg ctggcggcgt ggctcacgat
      121 tttaataacc tgctcatggt tatctccgga tatgccgata tggtggcgaa aaaactgggg
      181 aaggcgcacg ctctctaccg atttctggaa gaaattcagc aggccagccg ctcggcctca
      241 ggtgtgactc gccagttgct tgcctttagc cgcaaacagg atcaacaact caaatcgctc
      301 aacctgaccg acgtggtcac ccagcttcac aaattgctgc gccgcgtgat tggggaagac
      361 atcaagctgg tgacctctct ggctgatgat acggcaccgg tcctggctga ccctgctcaa
      421 atcgaacagg tggttctgaa tctggtggtc aatgcccgtg atgccatgcc aggtggcggc
      481 acactgacac tgcaaactga aaatgtggtt cgaacgactg aggaacaggc gctccatttc
      541 acggttccac ctggaaacta tgtcagctta actgttactg atactggatg tggaatggaa
      601 gaagccactc tggaacgaat ttatgaacca ttctttacca ccaaagaagc ccacaaaggc
      661 accgggttgg gtctgtcaac cgtctatgga atcgtccagc aatcaaatgg atatattctg
      721 gttgagagtc agccaggaac gggcaccagg tttacggtgt tgttgccaca aacaccgggc
      781 atggacctcc atcaggccac cgaagagcct gttgtcccaa ctgattccag caataccacg
      841 attctggtcg ttgaagatga agattcagtc cgaatcatgg tccgggaagt cctgacgttg
      901 tggggatatc gtgtcctgga agccaccagc ggcgaggccg ccatcaaaca aatggcccac
      961 caccacgacc agatcagtct ggtgctgacc gatgtggtga tgtctggaat gagtggggtc
     1021 aatctggtcc agcatatccg cgaaaaccac ccagcagtca aagttctgtt tatgtctgga
     1081 tatattgctg acgccagtga tgcggtcggg attgccagtc agctcgataa ttttatttcc
     1141 aaaccattta ccccgttgac actcgtcaaa gccattcaga cggttctcaa cgagagtgac
     1201 aaatgacaca cggtcgaggc ggtatctatc gcatttcagc taaatatttc ctgattttct
     1261 attttaggaa atttcagaaa ccattgaaga atctggattt attttctgtg tattccgtgt
     1321 attccgtggt ttcttattct gaaaacctat attttgtcat ctggtcattt ggggcccgca
     1381 ttcaaaatag ccaggatacg ctgaaccagc gctttggggg taaacggttt ctggagcaat
     1441 tccacccctt cgtccacgac gccatgatga gcaatcgcat tctcagtata gccggaaata
     1501 tagagtactg gcagattggg ctgtttttct ttcagccgtt tggccagcac cgggcccttc
     1561 attccaggca gcaccacatc cgtcagcaga aagtcaatct gaaggtgttg ggcttcaatg
     1621 agttccagcg ctgcttcggc agaactggct tcatacactc gaaacccggc gtctttgagg
     1681 atatggtggg tgaggtttcg gaccacgtct tcgtcttcaa ccagcagaat ggttgcggtt
     1741 tgggctggca gtaccggctg gtactcgcgc atttctggtg gctggtcaac ctcatgggtg
     1801 cgtggta
//
LOCUS       DAOQDO010001490         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68034_length_1807_cov_2.958904, whole genome shotgun sequence.
ACCESSION   DAOQDO010001490 DAOQDO010000000
VERSION     DAOQDO010001490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68034_length_1807_cov_2.958904"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKZ53_20060"
     CDS             <1..490
                     /locus_tag="PKZ53_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42793.1"
                     /translation="KWAEFQLIEPVVLNHQSKKFLEGVDSYPTHIREDSVALFLLTLG
                     ISTSVVIVATFLCESVFVFLFTSFLACYLSEIIVGPKLSRLKALNEKGKITLVEIDQV
                     TILSFPGLINPNHEYCVYFQFNIPDGPLVKCETRICTPYEIKPGNELVVLYLDEENYW
                     LL"
     gene            complement(487..1179)
                     /locus_tag="PKZ53_20065"
     CDS             complement(487..1179)
                     /locus_tag="PKZ53_20065"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNJ42794.1"
                     /translation="MSENTIEPIAILWDLDGTLIDSATYHFEGWRDMLASQGVAYTYD
                     DFLAHLGIRNDLFLKQYLGPDLPEAELTRLALVKEEAYRSKVRTNGLNLLPGAEAWLH
                     RVRANGWKQALASSAPRPNIDVAFEVIESFGLLDAHVSAEEVAHGKPFPDVFLAAAKK
                     LEVPIHRCIVVEDAPLGIEAARRAGMKVIGVLTTHTELEADLVVNTLEDLDDESFLGL
                     LQESELSVQTRA"
     gene            complement(1385..>1807)
                     /locus_tag="PKZ53_20070"
     CDS             complement(1385..>1807)
                     /locus_tag="PKZ53_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNJ42795.1"
                     /translation="DLRGTVITNSPPHATALAEHPHLQVILIGGGLLKESLVTIGAGT
                     VEAIRQIRADICFLGVCCVHHEAGITIPNLEESYVKRAMIAASAEVVALASTEKLETV
                     ATFAVAPLSELTHLVTESTAPEAVLQRYQQLGIKTMLV"
BASE COUNT          447 a          443 c          455 g          462 t
ORIGIN      
        1 aaaatgggct gaatttcagt tgatagaacc agttgtgctt aaccaccaat cgaaaaaatt
       61 tctggagggg gttgattcct atcctacaca catcagggag gatagcgtcg ctttatttct
      121 tctcacactt ggaataagca ccagtgttgt catcgttgcc acatttcttt gtgaaagtgt
      181 gtttgttttc ctgtttacaa gctttctagc ctgttatctg agtgagataa ttgttggccc
      241 caaactttct cgcctcaagg cattaaatga aaaaggaaaa ataacccttg ttgaaattga
      301 ccaggttaca atcttgagtt ttcctgggtt gataaatccc aatcatgaat attgtgtata
      361 ttttcagttc aacatcccag acgggcccct ggtcaaatgt gaaaccagga tttgcacacc
      421 ttatgaaata aagcctggga acgaactcgt ggttttgtat cttgatgagg aaaactattg
      481 gctgctttaa gcccgagttt gaaccgaaag ttcagattcc tggagcaacc ccaggaatga
      541 ctcgtcgtcg aggtcttcca gtgtattgac caccagatca gcttcaagtt ccgtgtgggt
      601 tgtcagcacg ccgatgactt tcatcccagc ccggcgggcg gcttcaattc caagcggagc
      661 atcttccacc acaatacacc gatggattgg gacttccagt tttttggccg ccgcaagaaa
      721 aacatccgga aatggttttc cgtgggcgac ttcttcggca gaaacgtgtg catcgagcaa
      781 accaaaagat tcgatcactt caaacgccac atcaatgttt ggacgcggag cggagctggc
      841 cagcgcctgt ttccagccat tggctcgaac ccgatgcaac caggcttcgg cacccggaag
      901 caggtttaac ccgttggtgc ggactttgga acggtatgct tcctctttga ccagcgccag
      961 ccgggtcagc tcagcttcag ggagatcagg ccctaaatat tgttttaaaa agagatcgtt
     1021 ccggatcccc agatgagcca ggaaatcgtc ataggtgtag gctactccct gacttgccaa
     1081 catatctcgc cagccttcaa aatgataagt ggctgagtct attaaagttc catccagatc
     1141 ccacaggatg gcaatgggtt cgatagtatt ttctgacatt gaatctcctt ttgaaaaacc
     1201 agggctgaag acttcgggct cggggctgaa gaaatcgggc tgaagaaatc gggctgaaga
     1261 aatcgggctg aagactcgca agctcggggc tgaagaaaat gggtttcata ctctgagcct
     1321 atcttcttcc gcccaatgtc ttcagccctt agcccctagc ccgaagtctt ccgcccgagg
     1381 ttctctaaac cagcatggtt ttgattccaa gttgttgata ccgctgaaga accgcttcag
     1441 gagcagttga ttcggtgacc agatgggtga gttccgagag cggcgccacg gcaaaggtcg
     1501 ccacagtttc cagtttttcc gtggaggcca gggccacgac ttcggctgaa gccgcaatca
     1561 ttgcgcgctt gacgtaggat tcttccaggt tcggaatcgt gattcccgct tcgtgatgga
     1621 cacaacagac gcctaaaaaa cagatatcag ctcgaatttg gcggatggcc tcaaccgttc
     1681 cagcgccgat ggtcacaagt gattctttca gcaatccgcc accgatgaga atgacttgga
     1741 ggtgtggatg ttcggccagg gccgtggcgt ggggcgggct attggtgatg accgttccgc
     1801 gcaaatc
//
LOCUS       DAOQDO010001491         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68037_length_1807_cov_2.894406, whole genome shotgun sequence.
ACCESSION   DAOQDO010001491 DAOQDO010000000
VERSION     DAOQDO010001491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68037_length_1807_cov_2.894406"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..>1807
                     /gene="pnp"
                     /locus_tag="PKZ53_20075"
     CDS             301..>1807
                     /gene="pnp"
                     /locus_tag="PKZ53_20075"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNJ42796.1"
                     /translation="MYVKQSIQFGGREFCLETGKIAKQANGSVIVRYGDTVLLVTAVG
                     AAEQRPDQSFFPLTVDYREYGYASGRIPGGYFKREGRPTERETLICRLIDRPIRPLFP
                     EGYMAETQVIAMVISADTNNDPDVLAITGASTALFISDLPFHTPIAGVRVGLIEGQFV
                     VNPNYQDMRNSELNLVLAGSEEAIVMVEAGAKEVSEETMVKAMVFGHSEIKKLCALQR
                     ELREKAGKAKREVAPPELDAEQLAEIEANFTGDIRTALDTRNRDKLTSYAELDALKKR
                     IAESYPEDAPAKRSMAKQIFEHLKEQIFRDDILNNRRRPDGRRFGEVRPISIEVGWLP
                     RTHGSCLFTRGETQAIVTTTLGTSEDIQYLDDLETGEVKRNFILNYNFPPFSVGETGR
                     MGTPGRREVGHGALAHRAIQAVLPGDEFPYVLRVVSDITESNGSSSMATICGGILSLM
                     DAGVPLKAPVAGVAMGLVMSDKKYAILTDIAGAEDHYGDMDFKVAGTANGIT"
BASE COUNT          439 a          479 c          489 g          400 t
ORIGIN      
        1 taatgaacgc tatcaccgac tcatcgccaa gcttggaatc cggaaataat ttacctgtac
       61 agtgtcagcc acttatctat aacgtcgctc aagtacaagc cgccagcacc ccgtcctctt
      121 gcgatcaaga acggggcctg ctgcggcttt ttgtgttcac gaactgaaag taccccacct
      181 gtcacggacc gtgggcggaa attcccagtt tgtgcgtcag gggcgagagg tcaatccccc
      241 ctgagaccct tgtgtgtctg agatcaacac acaactagat cggtagagga gaagatatat
      301 atgtatgtaa agcagtccat ccaattcgga ggacgtgagt tttgtcttga aactggaaaa
      361 attgccaaac aggccaacgg atcggtcatt gtgcgctatg gtgacaccgt gttgctcgtg
      421 acggcagtcg gcgccgctga acaacgcccc gatcaatcct tttttccatt gacggttgat
      481 taccgcgaat acggctatgc ctcaggccgg attccaggtg gctatttcaa acgtgaaggc
      541 cgccccaccg agcgtgaaac cttgatttgc cggctgattg atcgcccaat tcggcccttg
      601 ttcccagaag gctacatggc tgaaacccag gttatcgcca tggtgatctc ggcagatacc
      661 aacaatgacc cagatgtgct ggcaattact ggcgcatcca cggctctctt tatttctgac
      721 cttccatttc ataccccaat tgccggggtg cgggtcggtc tgattgaggg tcagtttgtg
      781 gtcaacccaa actaccagga tatgcgcaat tcggaactga acctggttct ggcgggcagc
      841 gaagaagcga ttgtgatggt cgaagccggc gccaaggaag tttcggaaga aaccatggtt
      901 aaggccatgg tctttggaca ttcggaaatt aagaaactgt gcgctctgca acgtgaactg
      961 cgtgaaaagg ctggaaaagc gaaacgcgaa gtggcgccac ccgaactgga tgcggaacaa
     1021 ctggccgaaa ttgaagccaa ttttactggc gatattcgga ccgcgctcga tacccgcaat
     1081 cgggacaaac tgaccagcta tgcggagctg gatgcgctca aaaagcggat cgccgaatca
     1141 tatccagaag atgccccggc caaacgcagt atggccaaac aaatctttga acacctcaaa
     1201 gaacaaattt tccgtgatga catcctcaac aaccgccgcc gtccagatgg tcgccggttt
     1261 ggggaagttc gcccaatcag catcgaagtt ggctggttgc cgcgcaccca cggaagttgc
     1321 ctctttaccc gtggcgaaac ccaggcgatt gtgaccacta cgcttggaac cagtgaagat
     1381 attcaatacc tcgatgatct cgaaaccggt gaagtcaaac ggaacttcat tttgaactat
     1441 aacttcccgc cgtttagcgt gggggaaacc ggtcggatgg gcacgcctgg ccgtcgtgaa
     1501 gttggtcacg gcgcactggc gcatcgtgcc attcaggcgg tgctccccgg agatgagttc
     1561 ccgtatgtgc tccgtgtggt ttcagatatc actgaatcca acggatcttc ttcgatggca
     1621 acgatttgcg gtggtattct gtcgttgatg gatgccgggg tgccgctcaa agcccccgtg
     1681 gctggggtcg cgatgggcct ggtgatgagc gacaaaaaat acgcgatcct gactgacatc
     1741 gccggtgccg aagaccacta tggcgacatg gacttcaaag ttgctggaac ggccaacggg
     1801 atcaccg
//
LOCUS       DAOQDO010001492         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68248_length_1804_cov_3.133791, whole genome shotgun sequence.
ACCESSION   DAOQDO010001492 DAOQDO010000000
VERSION     DAOQDO010001492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68248_length_1804_cov_3.133791"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKZ53_20080"
     CDS             complement(<1..536)
                     /locus_tag="PKZ53_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ42797.1"
                     /translation="MDDALAIDRWQFTFTITFHYLFPQLTMGLALLIVMLKSLALWKN
                     DDRFNRAAQFWGKVFGISFFFGVITGIPMEFQFGTNWARFARFAGGVIGQTLAMEGVF
                     AFFLESSFLGLFLFGEKRLGRWGHWLASVLVFLGSWLSGYFIIVTNAWMQHPVGYTLT
                     SDGSVHLSSFWALLLNPWA"
     gene            963..1520
                     /locus_tag="PKZ53_20085"
     CDS             963..1520
                     /locus_tag="PKZ53_20085"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNJ42798.1"
                     /translation="MTNVSHSLCIKYFLIGLLLGIVMLATASSALAQQPTGPQVRPKS
                     DQAESEPYTNRQPVFESLLRNPYESRLLAAALGLTEDQQTRIRQVYRQHGLTMASAFK
                     DMRARRIALDEALFSDEFNEAAVNQRAKDLAEAQTQLLMIQTKVQSQLRQILTNDQLH
                     KFMELRQDEGPRRFDKQLQKQMRKL"
BASE COUNT          484 a          452 c          446 g          422 t
ORIGIN      
        1 gcccacgggt ttagcagcag agcccaaaaa ctggagaggt ggacggaccc gtcgctggtc
       61 agggtgtagc caaccggatg ctgcatccag gcattggtca caatgatgaa atagcccgac
      121 agccacgaac caagaaaaac cagcaccgag gccagccagt gtccccagcg gccaagtcgt
      181 ttttcaccaa acagaaagag ccccagaaac gaggattcca ggaaaaaggc aaacacgcct
      241 tccatggcca gtgtctggcc gatgacacct ccggcaaagc gggcaaaccg cgcccagttg
      301 gtcccaaact gaaattccat ggggatgcca gtaatcacac caaagaaaaa gctgatgcca
      361 aagactttgc cccaaaactg cgcagcccgg ttgaaccggt cgtcattttt ccacaatgcc
      421 aggcttttca gcatgacaat caggagtgcc aaccccatcg tcagttgggg aaacaggtaa
      481 tgaaaggtaa tggtaaaggt aaattgccat cggtcgatag ccagggcgtc gtccatatcc
      541 agaaatgtac tttcttccca acagcgggac gtaacgacat ttcccatccg gtagcaaaag
      601 ccaggggtgt aagtagtaat aagggcaagc ggatcagtac cattccggat ggaaaacggg
      661 agaggaaaag aaccaaaccg aattctgttt tgcttgtgtt cccacgctat atcgctgatt
      721 gcttgaaaag caaatcaacc ttccgagcgc catttggcgg gtggcattgc tgatttgaaa
      781 tcggctggcc agttcggcca ctgaactgga ttagtcgttc tccgtgcttg attccgcatt
      841 gtggttttac ctgatttttg acaaactttt tcaggctgcg caggctctaa ccctggcaaa
      901 gaatccagtc actgaaaaga ttggtacagt tttacccagg cttgaaggat cacacaccca
      961 ctatgacgaa cgtgagtcac tcgctgtgca taaaatattt tctgatcggg ttgttacttg
     1021 gaatcgtgat gctggcgaca gcgtcaagtg cgctggccca acaaccgacc ggacctcaag
     1081 tccgacccaa atctgatcaa gccgaatcag agccctatac caaccgccag ccggtttttg
     1141 aatcattgtt gcgcaatccg tatgaatcgc gactgctggc cgcggccctt ggcctgaccg
     1201 aagaccagca gacgcggatt cgccaggtgt atcgccagca tgggttgacg atggccagtg
     1261 cgttcaagga tatgcgagcc aggcggattg ctctggatga ggcgttgttt agcgatgaat
     1321 ttaatgaggc ggctgtcaat caacgtgcca aagatctggc tgaagcacaa acccagttgt
     1381 tgatgatcca gaccaaagtg caatcccagc tccgccagat tttgaccaat gaccagctcc
     1441 acaaatttat ggaattacgt caggatgaag gaccacgccg ttttgataaa cagctccaaa
     1501 aacagatgcg aaagctgtaa gctgccagaa cctgcctgga acttgtaaaa aatcgtttgg
     1561 cagtttcatc agccatgaca tatagtttct ggacttcacc gatttgtttt tctgtctctt
     1621 tgtcgcaaaa caatttggtg taggcgtgaa gttgaatttg gaacattttt cacggtcgtc
     1681 tcctcacttt ggctctgaag ttgaatgcat acttcagagc ttttttgttt ccaaaatccc
     1741 agttttcgat ttgatttttg cctttcaaaa actcttcggg ccctgagttg gaaaggatga
     1801 agtc
//
LOCUS       DAOQDO010001493         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68334_length_1803_cov_2.635584, whole genome shotgun sequence.
ACCESSION   DAOQDO010001493 DAOQDO010000000
VERSION     DAOQDO010001493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68334_length_1803_cov_2.635584"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKZ53_20090"
     CDS             <1..200
                     /locus_tag="PKZ53_20090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HNJ42799.1"
                     /translation="RYSFGREAYQLLAPSAPIVDFSSQVMSVTWRQWTSKNWGVKVHV
                     EHYDNPSYRRTGLEMGVFKDF"
     gene            572..1636
                     /locus_tag="PKZ53_20095"
     CDS             572..1636
                     /locus_tag="PKZ53_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42800.1"
                     /translation="MSFKTLFLALMTLATITFGSTTSFAQDRFLRSVQSVAVTENHLY
                     IKDDVAQRVYRMVPGNNRGETFLRAGQLITFQDGSQHVLQSIGEYMLTPNGDFVVIDA
                     QAGRLIKRTASNGFEIVAEVGDPIFIPGTSDRIDTIEAISHIMFNAETNEILFSDNSL
                     RYVLRAAVGGTLLELDASRPVFVANLNDTVQIQDISNLSYATNPTRGGLIVLTDATSG
                     LVLTRDKYETNTLFGVLRSLTVFTNKALTSSRLESIEGVRELNFTNSNKVVLGNAATK
                     IIYRVDRFGIATADQKFDGNQIFTLPNGKLLTIGSMNDFAVTGENVFLADSAKLQVVK
                     VDQFGQVSLVASASETIFTE"
BASE COUNT          470 a          402 c          421 g          510 t
ORIGIN      
        1 ctcgctacag ttttggccgc gaagcctatc aactgctcgc ccccagtgcc ccgattgttg
       61 atttttcaag ccaggtcatg tcggtcacct ggcggcagtg gacgagtaaa aattgggggg
      121 ttaaagtcca cgttgagcac tatgacaacc cttcgtaccg ccgcaccggg ctggaaatgg
      181 gggtttttaa ggacttttaa acctggcctg ccggcatctg gagagggtgt gaatcagggc
      241 catcggtgat ttagcgagcg aatgatttaa atttaaatca ttcgctcgtt tttttatttt
      301 taaattaaat tatatcaagt aatttaattt aattttctaa attttctaaa tttttcaaag
      361 gaaaatttaa ttcaaggtca ggaatatatt taaacagatt tttttataat ttttaattaa
      421 ttcaaaataa aagagatatt caattttaat atttttccca cagtaaccgt gaatctttgg
      481 caccaacctt gcaatatgga aagtcacaca acagatcgaa agatcataac tcttcaaatt
      541 taattcgata acgaaaggtg actttacttt tatgtccttc aaaactttgt ttttggcgtt
      601 gatgaccctg gcaaccatta cttttggcag caccacttct tttgctcaag atcggttttt
      661 acgcagtgtg cagagcgtgg ctgtaaccga aaatcatctc tatattaaag atgatgtagc
      721 ccagcgagtg taccggatgg tgccaggcaa caatcgtggt gagacctttt tgagagctgg
      781 ccagctcatt accttccagg atggttcgca gcacgtgctc cagtcaattg gcgagtatat
      841 gttgactccg aacggtgatt ttgtggtgat tgacgctcag gcagggcgac tgatcaagcg
      901 caccgccagc aatgggtttg aaattgtggc tgaagtgggt gacccgattt tcattccagg
      961 caccagtgac cggattgata ctatcgaagc gatttcccac attatgttta acgccgagac
     1021 caacgaaatt cttttcagcg acaattcact gcggtatgtc ttgcgagcgg cggttggcgg
     1081 aaccttgctt gaactcgatg ccagccggcc agtgtttgtc gccaacctca acgatacggt
     1141 ccagattcag gatatttcca atctgagcta tgcgaccaac ccaacccgtg gagggctgat
     1201 tgtactgact gatgcaacgt cagggttggt cttaacacgt gacaaatatg aaaccaacac
     1261 gttgtttggg gtgttgcgtt cattgacggt atttaccaat aaagccttga ccagcagccg
     1321 gttggaatca atcgaagggg tgcgggaact caatttcaca aactcaaaca aagtcgtgct
     1381 gggcaatgcg gccaccaaaa tcatctaccg ggttgaccgg ttcggtattg ccacggctga
     1441 ccagaaattt gatggaaacc agattttcac actgcccaat ggcaagctgc tcacgattgg
     1501 ctcaatgaat gactttgccg taacgggtga aaatgtgttc ctggcagatt cagccaaatt
     1561 gcaggttgtc aaagttgatc agttcggcca ggtgagtctg gtggcaagcg cctcagaaac
     1621 aatcttcact gaataagatg actaagagat gatccacgtg attcattgac agctccccct
     1681 ccaaactcct ccttattctt gacgcagcct gttgtaccag caggctgcgt ttttttgtgt
     1741 acagcaaccc ggattggttt cggagtgcgg cggcttgacg ccgcttttac agttgccatt
     1801 cca
//
LOCUS       DAOQDO010001494         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68346_length_1803_cov_2.294622, whole genome shotgun sequence.
ACCESSION   DAOQDO010001494 DAOQDO010000000
VERSION     DAOQDO010001494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68346_length_1803_cov_2.294622"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKZ53_20100"
     CDS             complement(<1..542)
                     /locus_tag="PKZ53_20100"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNJ42801.1"
                     /translation="MKKHYPWLWFDADGTLFDYNQAEPIAIKNTFDALKLPFQDEYLA
                     IYRRINQQLWQALERGEITPAALHARRFELLMETIQLDFPPSQFGAAFMEQLALRDEL
                     IDGAHEVLHQLQEKCRVAIVTNGLQFVQRSRIALSPLKQYITELIISEEVGIAKPEAG
                     FFEIAAARTGHPPKNEVLIIG"
     gene            complement(805..1611)
                     /locus_tag="PKZ53_20105"
     CDS             complement(805..1611)
                     /locus_tag="PKZ53_20105"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42802.1"
                     /translation="MDAEVLAQEQHDAKKVDQAQLLIRDGQLDEAAARLREVIAHAPD
                     NHFSRFEVEKKLYIKCWDRAEFEYLVFLQQKTGDDRTLIWIPNAYPRAYYYLSLILMR
                     QNQFQEALEYLENGAVFEPKNPRFLLDKARAHGFLGHPQLAVQLYEQVLAMGVLILPA
                     LRAIAFRGKGVQLIELGELDQAEQALKQSLAIEPHNALAANELNHIANLRKSKHPGSL
                     LVAPMPLGTCALCQSSLDTDAGLLPINGLLQHVCSTCFKAEKPKWWQFWK"
BASE COUNT          461 a          391 c          450 g          501 t
ORIGIN      
        1 ccgatgatca agacctcgtt tttgggtggg tgccctgtcc gggcggcggc gatttcaaag
       61 aagccggctt caggtttggc gatgccaact tcttcagaaa taatcagttc cgtgatgtac
      121 tgcttgagcg gagacagtgc gatgcgactc cgttgaacaa actgcaaccc gttggtgaca
      181 attgccaccc gacatttttc ctggagctgg tgtaaaactt cgtgagcacc gtcaatgagt
      241 tcatcccgga gcgccagttg ttccataaat gccgccccaa actgactggg tggaaaatca
      301 agctggatgg tttccatcaa caattcaaat cggcgggcat gcagggctgc cggagtgatt
      361 tcacctcgtt caagtgcctg ccagagttgt tgattgatcc gccgatagat ggccaaatac
      421 tcgtcttgaa aaggcaactt cagagcgtca aacgtgtttt taatcgcaat cggctcggcc
      481 tgattgtagt caaaaagggt gccgtccgcg tcgaaccata accagggata gtgttttttc
      541 ataaaggttt agccatttct attgctgaat ttgattgata ggaacacatt ttgcgagaat
      601 gcaaagggcg actggctaca gaaaaaagct atagcaaaac gctgttagag aatttgccaa
      661 gtatggtggt ttgggatgtc tcaaggcaag aacggtaggc tgattttggt tggattacgc
      721 ctggtcactc ctcaaccttt ttgttgaaat cttcctgcca atcaacgaaa acgtgttatg
      781 attactgagt tatggggatg gtcgttattt ccagaactgc caccatttag gtttttcagc
      841 tttgaagcag gtactgcata catgttggag taaaccgttg atagggagaa gacctgcatc
      901 tgtatcaagt gatgattgac aaagggcaca ggttcccaat ggcatcgggg caactaaaag
      961 agaccctggg tgttttgatt ttcgaaggtt cgcgatgtgg ttcagttcat tggccgccag
     1021 agcattgtgg ggttcgatag caagtgattg tttcaaagct tgttccgcct gatccagttc
     1081 tcccaattca ataagttgaa ctccttttcc acgaaaggct atggctcgaa gtgccggtag
     1141 gatcaagact cccattgcca gaacctgctc atagagttga accgccagtt gtggatggcc
     1201 aagaaatccg tgagcgcgtg ccttatccag gagaaaacgc gggtttttgg gttcgaatac
     1261 cgcaccattc tcaagatatt ccagcgcttc ctgaaactga ttttgtcgca tcaagatcag
     1321 gctcaaataa taataagcgc gtggataggc attcggtatc cagatcaatg tccgatcatc
     1381 tcctgttttt tgttgcagga atacgaggta ctcaaactca gcccggtccc agcacttgat
     1441 gtacagtttt ttctcgactt caaagcggga aaagtgatta tctggagcat gcgcaatcac
     1501 ttctcgtaat ctggcagccg cttcatcaag ttgaccgtca cgaattaaga gttgagcctg
     1561 atcaactttt ttagcatcgt gctgttcttg agcgagtact tcagcgtcca tcgtttacct
     1621 acctgaaaag agtttgttga tttcatccat aggggagtca caaatgatct taatggttat
     1681 cttcaaggag agaaaagcat gaatttgcaa gccacgctgt attttcgaaa tgcaacttcg
     1741 gacaaggtct actttatcga gctgaaagaa tcgggcgacg gatatgtggt tgattttgct
     1801 tat
//
LOCUS       DAOQDO010001495         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68390_length_1802_cov_3.550086, whole genome shotgun sequence.
ACCESSION   DAOQDO010001495 DAOQDO010000000
VERSION     DAOQDO010001495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68390_length_1802_cov_3.550086"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKZ53_20110"
     CDS             <1..451
                     /locus_tag="PKZ53_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42803.1"
                     /translation="DFPSGRNPQPERRPSPEPIDPPTPAPTVNQKELRRVALRTRAKE
                     IMTRMSTIDSQIGLIKERLDELKREDQAVSPSQVKDGKTYLRAVGPRGEVVYIPVKTD
                     EDQRLKSRLQDLELDATRAQVEWEYLIDEVRRAGFTIQQVLKEDDAD"
     gene            559..1290
                     /locus_tag="PKZ53_20115"
     CDS             559..1290
                     /locus_tag="PKZ53_20115"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNJ42804.1"
                     /translation="MSPDLENLIALHEIDIRLVEFQAQIKALDTECTRIETEYQQYAV
                     AYNEVQSKLTSSHRRNTQLEADLADTQQKLEKYNEDQKRVRNQKEYAATLTEIDNAKK
                     AISRFETQLLELMDEVKRLEDEAAKYSPDIENKRKEADAAIADCRARQQQIKQDMEHI
                     KQERVRCEAAVSRPWMDRYNRVSKLRTGQALSQVINGACSSCRMTLRPQVYSEVRQGD
                     VIYICDHCSRILFYRPEPKVVVMEA"
     gene            1452..>1802
                     /locus_tag="PKZ53_20120"
     CDS             1452..>1802
                     /locus_tag="PKZ53_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42805.1"
                     /translation="MCQKHLKRDVLINLFCQEGTPMRMAVLWLLMLAAVVLSPLAWAQ
                     SGRVNESGTRSYSINAILQPVPDESGLPPLKGVESADPIPIPKNQVAVYEAGIEQEIQ
                     GFRSDVSGARYVLLV"
BASE COUNT          471 a          451 c          482 g          398 t
ORIGIN      
        1 agatttcccc agtgggcgca atcctcaacc tgagaggcgc ccatctcccg agccgattga
       61 ccctcccacg ccagccccaa cagtaaatca aaaagaattg cgccgggttg ctttacgaac
      121 ccgggcaaaa gagatcatga cgcggatgag taccattgat tcccaaattg gacttatcaa
      181 agagcggctt gatgagctga aacgtgagga tcaggcggtt tccccatccc aggtcaaaga
      241 tggaaagacg tatctcagag cagttggccc gcgtggcgag gtggtgtata tccccgtcaa
      301 aacggatgaa gaccagcggc tgaaatcacg tctccaggat ctggaactcg atgccacacg
      361 cgcccaggtt gaatgggaat atttaattga cgaagtgcgt cgtgctggct ttacgattca
      421 acaagtgctc aaagaagacg atgcagactg acccaccgga gcagggctcc gagtactccg
      481 ctcggatcat ttatccccga atgcgagtcg ccgctcctgc ttacctggtg ccgccgtctc
      541 agagttcagg aggtaaacgt gagccccgat ttggaaaatc taattgcgct gcatgaaatt
      601 gacatccgac tggttgaatt tcaggcacaa atcaaagcgc ttgatacgga atgcaccagg
      661 atcgagactg agtaccagca atatgctgtt gcttacaacg aagtccaatc aaaactgacc
      721 tcatcccacc gccgaaacac gcaactcgaa gccgatctgg ccgacactca gcaaaaactt
      781 gagaagtaca acgaggacca gaaacgagtt cgcaatcaga aagaatacgc tgccaccctc
      841 accgaaattg ataatgccaa aaaggccatc agccggtttg aaacccagtt gttggaattg
      901 atggatgaag tcaaacgtct tgaagacgag gccgccaaat attcccccga tatcgaaaac
      961 aaacgcaaag aagctgatgc ggcaattgcc gattgccgtg ccagacagca acagatcaag
     1021 caggacatgg agcacatcaa acaggaacgt gtccggtgtg aagccgctgt ctcgcgtccg
     1081 tggatggatc gctacaatcg ggtttcaaag ctgcgaactg gccaggctct gtctcaggtc
     1141 atcaatggcg cctgctcttc gtgccgtatg acgctcagac ctcaagtgta tagcgaggtc
     1201 cgccagggag atgtgattta tatttgtgat cactgcagcc gaattctgtt ttatcgtcca
     1261 gaacccaaag tggttgtgat ggaagcctga gcagcccctg actttcttca gatggctgat
     1321 caggcacgcc gctgaaggaa ggcactcggc aaaatatgtg ctcatccagt gtattttggc
     1381 ggctcaattc tgcattgaag ggaactgaag gcacttcagt gactgacgtt tcaggttggg
     1441 gattgtgagg cgtgtgccaa aaacacctga aacgggatgt gttgattaac ttattttgcc
     1501 aggaggggac gccgatgcgg atggcggtct tgtggttatt gatgttggcg gcagtggtac
     1561 tctcaccact ggcctgggcg caatcaggtc gggtgaacga atcaggaact cgttcctatt
     1621 caattaatgc cattctgcaa cctgttccgg atgaatctgg cctgcctccc ttaaaagggg
     1681 ttgagtcggc tgacccaatt ccgatcccta aaaaccaggt tgcggtgtat gaagccggga
     1741 ttgagcagga aatccaggga tttcgatctg atgtttctgg tgcgcggtat gtgctgctgg
     1801 tt
//
LOCUS       DAOQDO010001496         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68427_length_1802_cov_2.330853, whole genome shotgun sequence.
ACCESSION   DAOQDO010001496 DAOQDO010000000
VERSION     DAOQDO010001496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68427_length_1802_cov_2.330853"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PKZ53_20125"
     CDS             complement(<1..1386)
                     /locus_tag="PKZ53_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HNJ42806.1"
                     /translation="MANKNLFQSIPGNLIPQTNAVNNEGVRAYRFSPKHQLAQYTITG
                     CLNNTFYASAGQQLETVLELCKTIEPEFIAKTAVYCRERGFMKDMPALLMAVLSSKDR
                     DAFARVFPRVIDNGKMLRNFVQIMRSGVAGRKSLGSLPKRMVREWFETRTEDQIFDAS
                     VGQSPSFADILKMVHPKPGNEQREALFGYLIGRDINKNLLPDRIKLFEAYKAGYRAEV
                     PDIPFQMLTALELKTPEWTAIARNAPWQMTRMNLNTFARHGVFAQEGMTELIANRLRD
                     PKAVKRSRVFPYQLMTAFVSADSSVPVAVRDALQDAMEIALDNVPEFSGKVYVLPDVS
                     GSMSSPVTGYRPGATSVVRCIDVAALVAAAVLRQNPQAEVIPFEHRVVNIDINRRDSV
                     MTNAKKLASIGGGGTSCSAPLALLNSQKAKGDLVIYVSDNESWVDASRGRGTATMKEW
                     AVFKQRNPRARM"
     gene            1600..>1802
                     /locus_tag="PKZ53_20130"
     CDS             1600..>1802
                     /locus_tag="PKZ53_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR family
                     protein"
                     /protein_id="HNJ42807.1"
                     /translation="MAALQTVVIGLLGPTLDGGKGPTRWERWRPTVAVCQHENLLVHR
                     FELLYQEKFTELAEPIKGDIQSVS"
BASE COUNT          408 a          468 c          457 g          469 t
ORIGIN      
        1 catgcgcgcc cgtgggttgc gctgtttgaa cactgcccac tctttcatcg tcgctgttcc
       61 acgaccccgg cttgcgtcta cccaggactc gttgtctgaa acgtaaatca ccaaatcgcc
      121 tttggctttc tggctgttca gcaatgccag tggcgcgctg caacttgttc cgccaccacc
      181 aattgaggcc agttttttgg cgttagtcat cactgaatca cgccggttga tgtcaatatt
      241 gaccacgcgg tgctcaaatg gaattacttc cgcttgagga ttctggcgca acacggcggc
      301 tgcgaccagt gctgccacgt caatacaccg aaccacgctg gttgcgcctg gacggtagcc
      361 cgttaccggt gatgacatcg aacctgacac gtcaggcagg acatacactt ttccgctgaa
      421 ctcaggcacg ttgtcgaggg caatttccat tgcatcctgc aacgcatccc gaaccgcgac
      481 tggaacgctt gagtcggctg acacaaacgc ggtcatcaac tggtatggga acacacgtga
      541 ccgtttgact gcttttggat cacgcagccg gttggcaatc aactcagtca ttccttcctg
      601 agcgaaaact ccgtgccgcg caaaggtgtt caggttcatc cgggtcatct gccacggagc
      661 gttccgggca atcgctgtcc actcaggtgt tttcagctcc agagccgtca acatctggaa
      721 cggaatgtct gggacttccg cccggtatcc ggctttgtag gcttcgaaca atttgatgcg
      781 gtccggcaac aggtttttgt tgatgtcacg accgatcagg tagccaaaca acgcttcgcg
      841 ctgctcgttg cctggttttg ggtgaaccat tttcaaaata tcggcaaacg atgggctctg
      901 accaactgac gcatcaaaga tctgatcttc cgtgcgagtt tcgaaccact cgcgtaccat
      961 ccgttttggc aatgaaccca gtgatttacg acctgccacg cctgaccgca taatttgcac
     1021 aaagttgcgg agcattttac cgttgtcaat cacgcgaggg aacacacggg caaacgcatc
     1081 gcggtctttg cttgaaagca ccgccatcaa cagcgccggc atatctttca taaaaccacg
     1141 ctcacggcag tacacggcgg ttttcgcgat gaactcgggc tcaatggttt tgcacagttc
     1201 caacacggtc tcaagttgct gtccggctga cgcatagaag gtgttgttca aacagccagt
     1261 gatcgtgtat tgcgccaact gatgttttgg gctgaagcgg tacgcccgta cgccctcgtt
     1321 attgacggcg ttggtctgtg gaatcaagtt gccaggaatt gactgaaaca agtttttgtt
     1381 tgccattgtg atgttctcct tcggacaagt tttcgtttca ttccgatgtc ttcccatagt
     1441 gcacagggcg tgccagacgt actaagattt ccgaaaatat tttttaaata attgataata
     1501 aaaggaatat ttattatttg gttgatggtt ttgctttttg gtaagaaata tggtttgatc
     1561 attcttcgaa aattttctag ttaaagtaga gaaatagata tggctgcttt gcaaacagtc
     1621 gtaattgggt tgcttgggcc aacgctggat ggcgggaaag gtccgacgcg atgggaacgc
     1681 tggcggccaa ccgtggcggt gtgccagcac gaaaacctgc tggtccatcg gtttgaattg
     1741 ctctatcagg aaaagttcac cgaactggcc gaacccatca aaggcgatat ccagtcggtt
     1801 tc
//
LOCUS       DAOQDO010001497         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68537_length_1800_cov_3.493410, whole genome shotgun sequence.
ACCESSION   DAOQDO010001497 DAOQDO010000000
VERSION     DAOQDO010001497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68537_length_1800_cov_3.493410"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1470)
                     /locus_tag="PKZ53_20135"
     CDS             complement(217..1470)
                     /locus_tag="PKZ53_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42808.1"
                     /translation="MHVPRGRPIHENLSTSYVNLAALLAELQISEFTGYVSMNFWNYE
                     GYVFLDNGQLINAYERAEALTKRGREAVDSILSRSQARGGAISFFHHPETVIRALAGI
                     INGEVVYRELASEFTHLERLVDKLKKSPEVIWYVEVLLDREAGAGVIYIIDGKAEAVV
                     SIRENAESNVLRTTTGPEGLMVLVAQVNSGGGVFNVYRDAGTTSAPASPGVGYHQSLP
                     QLTAGGGSSSLLPAVSTANVTAISSTRATSSRMETEPATDSLVVALEDDDRDASPVEE
                     VHGSPVSLTTEQYSELVGLMGDVMAAVERGIVDVVRETDFRLAFREAAIRAAEHYPFL
                     DPFAGEFEYTAGEIVFLGAARPVDFVVGLSQALRWTLDDLSRRVTDVDLEARVEATLK
                     NLQRNRREDFEKFGLESAIEAILGH"
BASE COUNT          469 a          489 c          452 g          390 t
ORIGIN      
        1 atatgactcc acttattgtt tgaatcgctg ggccggctga agtcacagcg tgggaaaaac
       61 gcttttgagg agcagacctg gaatatccag attgaacggg cgacaattgc ggctggcaca
      121 cagaaatgaa ctggcttcaa gcaccttcca actgccccag gcactgactc acgttgtatc
      181 aaaaacaaag gcgaaaagcc aagtcttcgt ggttaattaa tggcctaaaa tggcttcaat
      241 ggcgctttcc agaccaaact tttcaaagtc ttcacgtcga ttgcgttgca agtttttgag
      301 tgtggcttca acccgggctt ctaagtcaac gtctgtcacc cgccgactca ggtcatcgag
      361 cgtccaccgc aaagcctgtg agaggcccac cacaaaatca actggccggg cagcccctaa
      421 aaaaacaatt tctccggcag tatactcaaa ctctccggca aaggggtcca aaaacggata
      481 gtgctcagcg gctcgaatcg ccgcctcacg aaaagccagc cgaaaatcag tttcacggac
      541 aacatccaca ataccgcgtt caacggcagc catcacatct cccattaacc caaccagctc
      601 tgagtactgc tcagtggtta aagacaccgg agagccgtga acttcttcaa caggtgaggc
      661 atcgcggtcg tcatcttcca aagccaccac cagcgaatca gttgctggtt cggtttccat
      721 ccgggaagaa gttgcccgcg tcgagctgat ggcggtcaca ttggcggttg aaactgccgg
      781 aagcagcgaa cttgaacccc ctccagcggt taactgtggc agtgattgat gatacccgac
      841 acccggcgac gcaggcgcac tggtggtgcc ggcatcccga tagacattaa agactccacc
      901 acctgagttg acttgcgcca cgagcaccat gagtccctca ggtccagttg tcgttcgaag
      961 cacattgctt tcagcatttt cccgaatcga caccacggct tcggctttcc catcaataat
     1021 gtagatcact ccggccccag cctcacgatc taacagaacc tcgacatacc agatcacctc
     1081 aggtgatttt ttgagcttgt caaccaggcg ttcaaggtgg gtaaattccg aggccagttc
     1141 ccggtaaacc acttccccat tgatgatacc agccagcgcg cgaatcacag tttccggatg
     1201 atgaaaaaag ctaatcgccc caccgcgagc ctgcgagcgg gagagaatgc tatcaaccgc
     1261 ctcacgtccg cgtttggtca acgcttcggc tcgttcatag gcattaatga gctggccatt
     1321 gtcgagaaac acatagcctt catagttcca gaaattcatc gacacatagc cggtaaattc
     1381 actaatctgg agttcagcaa gcaaagcggc aagattaacg taggaggtgc tgaggttttc
     1441 gtgaatgggt cttccgcgtg gaacgtgcat gattgaaact gagaagtgag gaggtaaatt
     1501 gataaccgcc ctatgggcac aaggtgagct ggctgtatgt agttgatgag agtgaatcaa
     1561 atcgctcaga ctggcttgtt tgagcgatgg cctgagcgac cagggcccag tgagcagttg
     1621 cctcaggtga ggtgtcaggt atctcggttt ttggatgggc acgctgggag tgagcctccc
     1681 tcgaaccacc ggatcaatct gattaggcaa aaagctgccg ggtacagaat ccacagatga
     1741 tggtcagtga ctgaatatgc cgtgatcaaa tacccagcat tccagtaccg cccagaggtc
//
LOCUS       DAOQDO010001498         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68594_length_1799_cov_4.452982, whole genome shotgun sequence.
ACCESSION   DAOQDO010001498 DAOQDO010000000
VERSION     DAOQDO010001498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68594_length_1799_cov_4.452982"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PKZ53_20140"
     CDS             complement(<1..761)
                     /locus_tag="PKZ53_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42809.1"
                     /translation="MISLQMHSKSQLRRKWLVAVALVVLVGYGVITGLAAWYLPNILL
                     FAWTPHRTEAQLETIRSKYSLPGDFWTRHEIKGGEGVPIELHWLHRKQSKGVAIHLHG
                     VFDDAWGASSPRLGELPDWDAVLFTFRGRDKHPEVPCTLGAWERFDVVAAAQFLASQN
                     QALNRIIIVGSSMGAGTALLALRELENQGTVLAGALLESPFQDVPTAASNHIHNAIGP
                     AELLLRPALIIGLHRLKSLARFDPFTISPLKASQGL"
     gene            1065..1625
                     /locus_tag="PKZ53_20145"
     CDS             1065..1625
                     /locus_tag="PKZ53_20145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ42810.1"
                     /translation="MSDAAIDRILLEQAIAGDEAAFSQLYERHWELVYRFAFRIVNSV
                     EAAQDITHDCFVSLIKHPDRYNPNLASLSTYLCAAARNLALKQFRRTETELLSEDEAF
                     APNQPGGDRSPLQQVLDRELSAVVQQAIAELPVLQREALVLFEFEELSLAEVATVVGT
                     DVGTVKSRLSRARERLKKVFAPYFQP"
BASE COUNT          437 a          514 c          428 g          420 t
ORIGIN      
        1 agcccctgtg aagccttgag tggcgaaatg gtaaacgggt caaacctcgc cagtgacttg
       61 agtcggtgca acccaatgat caacgctggc cgcaacaaca gttccgctgg cccaatcgcg
      121 ttgtggatgt gattgctcgc cgctgtcggg acatcctgaa atggtgattc aagcaacgct
      181 ccggccagca ccgttccctg attttccagt tcccgaagcg ccagcaatgc cgttcccgcc
      241 cccatgctgc tgccaacaat gatgattcgg ttcagcgcct gattttggga agcaagaaac
      301 tgggctgccg ccacgacgtc aaagcgttcc caggcgccaa gtgtgcaggg gacttccggg
      361 tgtttatcgc gtccgcgaaa ggtaaacagc accgcatccc agtcgggcaa ctctcccaat
      421 cgtggagacg acgcacccca ggcatcgtca aacaccccgt gcagatgaat cgcaacacct
      481 ttcgattgtt tgcgatgcag ccagtggagt tcaatcggga cgccttcccc gcccttgatt
      541 tcgtgccgag tccagaaatc tccaggcaag ctatatttgg aacggattgt ttcaagctgc
      601 gcctcagtcc ggtgcggtgt ccaggcaaag agtagaatat tggggagata ccaggcagcc
      661 agcccggtga tgacgccata tccaaccagc actaccagcg ccaccgcgac cagccatttt
      721 cgtcgaagtt gtgattttga atgcatttga agcgaaatca tggattggtt cctccgaaaa
      781 tatttgattg aaggcgtatc ttttctctga tttatggtaa taagcagcag aatctcaatc
      841 gggattccgg gttccgggtt cagggttcag ggttcaaagt cttcgaaatt tatgtctttt
      901 tcgtcctttt tgacttttcc aaaccctgaa ccctaaaacg gttttaaact ggcagtatca
      961 aactgaatac cagctattta gcaaattctg cattcaagcc cacccgctta cgcaggtggt
     1021 actgactctt cattttttcg agagagcaca tcacatcact caacgtgtct gacgctgcaa
     1081 ttgatcgcat tcttttagaa caggcaattg ctggcgacga agctgccttt tcgcagcttt
     1141 atgaacgcca ctgggaactg gtgtatcgtt ttgcctttcg tattgtcaat tcggtcgaag
     1201 ccgcgcaaga cattacacac gattgcttcg tcagcctgat caaacatccg gaccgctata
     1261 acccaaatct ggcgtcgctg agcacctatt tgtgtgcggc ggcccgcaat ctggcgctca
     1321 aacaatttcg ccggactgaa actgaacttt taagcgaaga cgaagccttt gcccccaacc
     1381 agccaggcgg ggaccgaagc ccgctgcaac aggtacttga ccgcgaactc tctgccgttg
     1441 tccagcaggc aattgccgaa ctcccggtgc tccaacgcga agcgcttgtg ttgtttgaat
     1501 ttgaagaact ttcgctggcc gaagtcgcca ccgtcgtcgg cactgacgtc ggcacggtca
     1561 aatcacgact ttcccgcgcc cgcgaacggc tcaaaaaggt ctttgcacct tattttcaac
     1621 cctaaaaacc agggttcacg gttcctggaa aagacataaa ggacgaaaag gacataaagg
     1681 accaaagttc aagaactcta aaccctggct ttcaaaccct gaaccctgaa ccctgaaccc
     1741 tggttctcaa accctgaacc ctgaaccctg aaccctggct ctcaaaccct gaaccctga
//
LOCUS       DAOQDO010001499         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68609_length_1799_cov_3.056193, whole genome shotgun sequence.
ACCESSION   DAOQDO010001499 DAOQDO010000000
VERSION     DAOQDO010001499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68609_length_1799_cov_3.056193"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKZ53_20150"
     CDS             complement(<1..364)
                     /locus_tag="PKZ53_20150"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNJ42811.1"
                     /translation="MTQRFADIAFTTSVKAKQEHYRTRRQNQRLEEADWGLDGLTETE
                     AQFISERDGFYLASVNEEGWPYVQFRGGPKGFLKVLDSHTLGYADFRGNLQYISMGNI
                     SHDNRVSLILMDYARKRRI"
     gene            complement(560..1120)
                     /locus_tag="PKZ53_20155"
     CDS             complement(560..1120)
                     /locus_tag="PKZ53_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ42812.1"
                     /translation="MSRLNVVDRLTTQPEIKPLFDAVESQLGGVPNFLRVFANSPKAL
                     AGILALNGNLGQGLLNAKLQEQIALTVGEANACQYCVSAHTAISQSLGLDATEIEAAR
                     QGNASDEKASAAVQFAKALVDNDGNITTGELKAVRDAGYSDGEIVEIITLVGLNTTLN
                     YLGKVSRVEIDFPEVTLLSRSTAVAG"
     gene            complement(1171..1614)
                     /locus_tag="PKZ53_20160"
     CDS             complement(1171..1614)
                     /locus_tag="PKZ53_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ42813.1"
                     /translation="MTNEAAKSVPLVPPFTRETALAKVKAAENAWNSRDPERVALAYT
                     LDSEWRNRTEFFTGREAITAFLQRKWAKELDYHLMKELWAFTENRISVRFEYEWRDAE
                     TGHWMRTHGNEHWEFDEQGLMRRRDMSANDYPIQESERRIFPASR"
BASE COUNT          434 a          477 c          449 g          439 t
ORIGIN      
        1 tgatccgccg ctttcgggca taatccatca aaatcagtga cacccggttg tcgtgactga
       61 tgttgcccat gctgatgtat tgcaaattcc cgcgaaaatc agcgtagccc agggtgtgcg
      121 aatccaaaac tttcaaaaat cctttgggtc caccacgaaa ctgcacatag ggccagcctt
      181 cctcattgac cgaagccaga taaaacccat cgcgctcact gatgaactgg gcttcggttt
      241 cagtcagtcc atccaatccc caatcggctt cttccagacg ctggttctgc cggcgggtgc
      301 gatagtgttc ctgtttggct ttgacactgg tggtaaaggc aatatcggca aatcgttgag
      361 tcatatgatc ctcgggttga agaaaatggg ctgaagacaa cgggatgaag acaacgggat
      421 gaagattcgc aagctcaggg ctgggggctg aagacaacgg gctgaagata acgggctcag
      481 ggatgaaaga aaaccagttc ttctgcccga cttcttctgc ccaacttctt cagcccccag
      541 cccttagccc caaatggcct tacccagcca cggcagttga ccggctcagt aacgtcactt
      601 ctgggaagtc aatttcgacc cggctcactt tgcccagata gttcaatgtc gtgttcaaac
      661 caaccagggt aatgatttcg acaatttcgc cgtctgaata tccggcatcc cgaacggctt
      721 tcaactcacc agtggtgata ttgccgtcgt tgtcaaccaa cgccttggcg aactgcacag
      781 cggcactggc tttttcatcg ctggcatttc cctgccgggc agcttcgatt tcagtggcat
      841 ccaggccaag tgactggctg atggcggtgt gagctgaaac acaatactga caggcattgg
      901 cttcaccaac ggttaacgca atctgttcct gtaacttcgc gttgagcaag ccctgtccca
      961 gattaccatt gagcgccaga attccggcta atgctttcgg tgaattggca aacacacgca
     1021 gaaaattggg aaccccaccc aactgtgatt cgactgcgtc aaacaagggt ttgatttcgg
     1081 gctgagtggt caaacgatca acaacattta aacgtgacat tggaaaatct cctctatata
     1141 aattttggta atcagttaag aaacaaaggt ttatcggcta gctgggaaaa ttcgacgctc
     1201 tgattcctga atcggatagt cgttggcgct catatcgcgc cgccgcatca gaccctgttc
     1261 atcaaactcc cagtgttcat tgccgtgggt gcgcatccag tgaccggtct cagcatcccg
     1321 ccattcgtat tcaaatcgaa ctgaaatccg gttttcagta aatgcccaca gttctttcat
     1381 caagtgatag tcaagttctt tggcccattt gcgctgcaga aatgccgtga tggcttcacg
     1441 accagtgaaa aactcggttc ggttgcgcca ctccgagtcc agggtgtagg ccagtgccac
     1501 ccgctccggg tcacggctat tccaggcatt ttccgccgct ttgactttgg ccagggccgt
     1561 ttcccgcgta aatggcggca ctaaggggac agatttcgcg gcttcgttcg tcatagaatt
     1621 ctccgggttt tggggctgaa gacttcgggt tgaagaaaac gggttgaaga aattgggctg
     1681 aagattcgca agctcggggc aatcgaaggg atgaaggatg aaggatgaaa gaaaaccagt
     1741 tcttcaacct gctttcttca gcccccagcc ctgagcccaa tgtcttcagc cccgggttt
//
LOCUS       DAOQDO010001500         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68621_length_1799_cov_2.685206, whole genome shotgun sequence.
ACCESSION   DAOQDO010001500 DAOQDO010000000
VERSION     DAOQDO010001500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68621_length_1799_cov_2.685206"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PKZ53_20165"
     CDS             <1..1008
                     /locus_tag="PKZ53_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42814.1"
                     /translation="GPLPLGNFTNLDLNNPSGVTVFAQLDTNVNSYVQQWSMQVQRQL
                     NSETALSIGYVGTQGKKLVSYYNLNRNFFNAPGGARPFPRLGDVNVAESRGNSIYHSL
                     QVQLERRLTKGWQYLMSYTWGHAIDDSRSGFSASNQPQDVRNFRLERGSADVDSRHRF
                     VFSSLYELPFGRGRQFGANMNRVADLFVGGWQVNGILTLQSGFPFDLNTNGNPGQVRP
                     DLVGSPSVNSDTVDRYLVRGQFREVEKNGDGVFLRPGGAGRNFLVGPGTKNFDLSLFK
                     SFAITERIRTEFRTEFYNLTNTPQFSQPNTNVSDGNFGRILGTRLGTNRQIQFALRVV
                     F"
     gene            1104..>1799
                     /locus_tag="PKZ53_20170"
     CDS             1104..>1799
                     /locus_tag="PKZ53_20170"
                     /inference="COORDINATES: protein motif:HMM:NF020653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ42815.1"
                     /translation="MSHGTHIRKKLAPQPSWWRLSILCFGLIFFPLTFVKAPFRLVSA
                     ASPHFQKTTPHILKTEPPNWWTVSRPTTVQVLLTGTNLKKAKVQTSTKGVSVGQTSAS
                     PNGNYLFCDLLLTPEATAGTVELEITADSTTIQKSWPLFSPLPREGNYQGLLPSDIVY
                     LIMPDRFADGDPSNNEPAGPGQTYNPRESRMYHGGDLRGVIQHLDYLKELGVTAIWLT
                     PVYDNTDTKGDVYH"
BASE COUNT          428 a          510 c          436 g          425 t
ORIGIN      
        1 gggccactcc cgttgggcaa cttcaccaat ctggatctca acaatccaag tggtgtgacg
       61 gtatttgcgc aacttgatac caacgtcaat tcctacgttc aacagtggag catgcaggtt
      121 cagcgccagt tgaattctga aacagcactg agcatcgggt atgttggaac tcaaggcaag
      181 aaactggtca gctactacaa tctgaaccgc aacttcttta acgctccagg tggtgcacgt
      241 ccgttcccac gcctggggga tgtcaacgtc gccgaatcac gcggaaattc aatttaccac
      301 tcgctgcaag tgcaattgga acgccgtctg accaaaggct ggcagtacct gatgtcttat
      361 acctggggtc atgccattga tgattcccgg agcggatttt ccgccagcaa ccagccgcaa
      421 gatgttcgca acttccggct tgaacgcggt agcgcggatg tggattcacg gcatcggttt
      481 gtcttcagtt cactgtatga actgccattc ggtcgtggcc gccagtttgg cgccaacatg
      541 aaccgggttg ctgatttgtt cgtcggtggc tggcaggtga acgggatttt gacgctgcaa
      601 agcggattcc catttgacct caacaccaac ggcaacccag gtcaggtccg ccctgatctc
      661 gtgggaagcc caagtgtgaa ttccgatacc gtggaccgat accttgtccg tggtcagttc
      721 cgcgaagttg aaaagaatgg tgatggcgtg ttcctgcgtc cgggtggcgc aggccgtaac
      781 ttcctggttg gtccagggac caagaacttt gatctgtcgc tctttaagag ctttgcaatt
      841 actgagcgga ttcgcaccga attccgcact gagttctata acctgaccaa cacaccacaa
      901 ttctcacaac ccaataccaa cgtcagcgat ggaaacttcg gtcgcatcct gggcacccgc
      961 ctgggcacca accgacagat tcagttcgcc ttgcgcgtgg tgttttaact gaaaggatgg
     1021 ggagtcctgg gaggaaccgg gccttgcacc cgctcctccc accccgtcca cgtcactcag
     1081 ctctcaagtc aggggatcct accatgtcac acggcacaca catcagaaag aaattggcac
     1141 ctcaaccatc ctggtggcga ctttcaatcc tgtgctttgg cttgattttt tttccgctga
     1201 cgttcgttaa ggctccgttc aggttggttt ccgcagcatc tcctcatttc caaaaaacaa
     1261 cgcctcacat cctgaaaact gaaccaccaa actggtggac cgtttccaga ccaaccacag
     1321 ttcaggtcct tttgaccgga acaaatctaa aaaaagcaaa agttcaaact tcgaccaaag
     1381 gggtttcagt tggtcaaact tcggcttccc ccaatggaaa ttacctgttc tgtgatctct
     1441 tgcttacgcc cgaagcaacc gccggaacgg ttgagctgga aatcacggct gattcaacga
     1501 cgattcaaaa atcctggcca ttattctcgc ccctcccgcg tgaggggaat tatcaaggtc
     1561 ttttgccatc tgatattgtg tatttaatta tgccggaccg gtttgccgac ggagacccct
     1621 ccaacaacga gccagccggt cctggccaaa cctataaccc cagggaatct cgcatgtacc
     1681 acggcggaga cctgcggggc gtgattcaac acctcgatta tttaaaagaa ctgggcgtca
     1741 ccgcgatctg gctcacccca gtctatgaca ataccgacac caaaggtgat gtctatcat
//
LOCUS       DAOQDO010001501         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68723_length_1798_cov_1.907631, whole genome shotgun sequence.
ACCESSION   DAOQDO010001501 DAOQDO010000000
VERSION     DAOQDO010001501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68723_length_1798_cov_1.907631"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKZ53_20175"
     CDS             <1..491
                     /locus_tag="PKZ53_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42816.1"
                     /translation="QSALSIPSLGTGVLTTSQHFLPIDVGGENPGNLYYLGIRLITTD
                     SNTENNQTQQWDMMPVFVPRAKTLLNLVSEVTGKEKETISIPVTLLGSRYYKPIAGQT
                     VQFFLDDQILGDGVTDQQGKAVLTYKLPKKSAGIKTLKVKYLGASAYEPAEASAELKI
                     VS"
     gene            747..>1798
                     /locus_tag="PKZ53_20180"
     CDS             747..>1798
                     /locus_tag="PKZ53_20180"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ42817.1"
                     /translation="MQRWVVILVLAMICAVPNYIGVQGFVVPQDRGQRVSGSTGARNT
                     TRKLALVIGNSAYTEAPLKNPANDASDMAVALKQLGFEVMSAINVNQREMKRLIREFG
                     GQLRTGGVGVFFYAGHGMQVNGRNYLIPIGATINTEPDVEDEAVDVNLLLGQFEMAGN
                     GLNIVILDACRNNPFGRSFRSVEKGLAQMTAPTGTVVIYATAPGRVASDGNTRNGLYT
                     QELLTNLRQPGMRLEDILIQTRVAVKTKSGGQQVPWENGALEGVFYFVPPGANSGGGS
                     SGSSVTAETVEEDAWNEIKDSPRAEDFKVFLREFPNGKRAPLARVKLAKVESPSVPIK
                     PVLTAGSRAQYGGMEFV"
BASE COUNT          466 a          416 c          512 g          404 t
ORIGIN      
        1 ggcagagtgc cctttccatc cccagtttgg gaactggcgt actgaccaca tcccagcatt
       61 ttttaccgat tgacgtgggt ggtgaaaatc ccgggaacct gtactatctc ggtattcgac
      121 ttattaccac tgattcaaac accgagaaca atcaaactca gcaatgggat atgatgccgg
      181 tttttgtacc gcgagccaaa accctgctga acctggtttc agaagtaaca ggcaaagaaa
      241 aagaaaccat ttcaattcct gttactttgt tgggaagccg gtattacaag cccattgctg
      301 gccaaacagt tcagtttttc ctcgacgacc agatactggg tgacggtgta acggatcaac
      361 aaggcaaagc tgttttgact tacaagctcc cgaaaaagtc ggctgggatc aaaaccttga
      421 aggtgaaata tcttggagcc tcggcctatg aacctgccga agccagtgcc gagcttaaaa
      481 tcgtttccta actccaattg cagatttgcc tcaatgggca actgaaagga ggctggcata
      541 gaatgtcgag ctcctttcag tcgcccagcg ggatggagta ttaacttcag atcacctttg
      601 cacaatggag gctcacccct ttccgacttg acaagattca agtaagtgtc agagactgag
      661 cgttaatcca gttctctact ggtttttttc cgggttgggt tcagcaactc aggttgttta
      721 tcgaaggaaa agttggaggg ccaggtatgc agcgatgggt tgtcatcctc gttttagcga
      781 tgatatgtgc cgtaccgaat tacattggag tgcaagggtt tgtggtaccc caggatcgtg
      841 ggcaacgggt ttcaggctca actggtgcca gaaataccac gcgcaaactg gcgcttgtca
      901 ttggcaacag tgcctacacg gaagcgccat tgaaaaatcc cgccaatgat gcctccgata
      961 tggccgtagc gttgaaacag cttgggtttg aagttatgag tgccatcaac gtcaatcagc
     1021 gggaaatgaa acggttgatt cgggaatttg gaggacaact cagaaccggg ggtgtcggcg
     1081 tcttttttta tgccgggcac ggaatgcagg tcaacggacg aaattacctg ataccgattg
     1141 gcgcgacgat caatacggaa ccggacgtgg aagacgaagc ggtggatgtc aatttgctgc
     1201 tgggacagtt tgagatggca ggaaacgggt taaacatcgt gatcctggat gcgtgccgga
     1261 ataatccgtt tgggcggagc tttcggtcgg ttgaaaaagg gctggcacag atgaccgcgc
     1321 cgacggggac ggtggtgatt tacgcgaccg caccgggaag ggttgccagt gatggaaata
     1381 cccgcaatgg gctttacacc caggagttgc tgaccaattt gagacaaccg ggaatgcggt
     1441 tagaggatat tctgatccag acccgagtcg ccgtgaaaac gaaaagtgga gggcaacagg
     1501 tgccttggga aaacggagcc ctggaggggg tgttttactt cgtcccacca ggagccaata
     1561 gcggcggcgg aagcagcggg tcatccgtca ctgccgaaac ggtcgaggaa gacgcctgga
     1621 atgaaatcaa agacagcccg cgtgctgaag atttcaaagt cttcctcaga gagtttccca
     1681 atggcaaacg cgcaccgctg gcccgagtca aactggcgaa ggtggagtca ccttcagtcc
     1741 cgatcaaacc agtgctgaca gcgggaagcc gggcgcagta tggaggaatg gaatttgt
//
LOCUS       DAOQDO010001502         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68772_length_1797_cov_2.639495, whole genome shotgun sequence.
ACCESSION   DAOQDO010001502 DAOQDO010000000
VERSION     DAOQDO010001502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68772_length_1797_cov_2.639495"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKZ53_20185"
     CDS             <1..366
                     /locus_tag="PKZ53_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42818.1"
                     /translation="GEEFVVVLVGGAPTEAYAIAERLRIKVAETPIWIGDRNLHVTFS
                     GGLADCASTPTYLQVLKDLQSQSNHWIESGSQSQSETEILANEMIELADQHLYRAKQA
                     GRNRICGSVSLETQTVATA"
     gene            950..1036
                     /locus_tag="PKZ53_20190"
     tRNA            950..1036
                     /locus_tag="PKZ53_20190"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:983..985,aa:Tyr,seq:gta)
     gene            1157..1232
                     /locus_tag="PKZ53_20195"
     tRNA            1157..1232
                     /locus_tag="PKZ53_20195"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1191..1193,aa:Trp,seq:cca)
     gene            1255..1340
                     /locus_tag="PKZ53_20200"
     tRNA            1255..1340
                     /locus_tag="PKZ53_20200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1288..1290,aa:Ser,seq:tga)
     gene            1465..1558
                     /locus_tag="PKZ53_20205"
     tRNA            1465..1558
                     /locus_tag="PKZ53_20205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1497..1499,aa:Ser,seq:gct)
     gene            1567..1641
                     /locus_tag="PKZ53_20210"
     tRNA            1567..1641
                     /locus_tag="PKZ53_20210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1602..1604,aa:Arg,seq:gcg)
     gene            1657..1732
                     /locus_tag="PKZ53_20215"
     tRNA            1657..1732
                     /locus_tag="PKZ53_20215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1690..1692,aa:Leu,seq:tag)
BASE COUNT          431 a          442 c          430 g          494 t
ORIGIN      
        1 ggtgaagaat ttgtggtggt gttagtcggc ggggcgccaa ctgaagccta tgccattgca
       61 gaacgtctgc ggatcaaagt ggcagaaact ccgatttgga ttggtgatcg caatctgcat
      121 gtcacctttt caggtggact cgccgattgt gcgtcaacac cgacttatct ccaggtgctc
      181 aaagaccttc aatctcaaag taaccactgg attgagtcag gatcccagag tcaatctgag
      241 actgagatcc tggccaatga aatgattgaa ctggcagatc agcacctgta ccgggccaag
      301 caagcaggac gcaatcgaat ttgcggctcg gtcagtcttg aaacccagac cgtcgccacg
      361 gcctgagcct ggagactcca ggctcagagt atcaggattt tcggacaagg attgaatgaa
      421 taatttcttt tacatattga tttatttatc aattaaatat tttttggaga ttatttaaaa
      481 aaacttgttg acatcatttt tttcctatgg tagatattcg ctatcgaaat ttacttactc
      541 tttttgcgag gtatgccatg cggcgctcaa cattttcggc catccagata agcgttcatc
      601 gcaccgtcaa gttgacggca ggacagccat gcgccgcgac cgggaccgga tatcaagcct
      661 ggtccaggat tgaacaaccc aaatttgaaa cgctgcaggg tagatttgtg ggcttaggtt
      721 gcctcgatca cctgaatatg gaaccgaaag gtagcgtcat tcttcgtagc agcgataaag
      781 cagtgtgaca ccgcgggagc ggatttactc ccgctcccga aactctcaaa atcaaattga
      841 tctgagattg tctaaggagc ggggccgctt tccacccaga aagcccccgc tccttttctg
      901 ttttggggtc tttgacaact aaatatctac ccttttttga agtatttatg caggagaggt
      961 cgaattggta cgacaacggt ctgtaaaacc gttctccttg tggagtatgc aggttcaaat
     1021 cctgcctcct gcaccagctc aattcagaag gatgaagcaa gccgaagcaa ggatgagctg
     1081 cgggctgatt tccaggccaa cgaaatcccc ccatctcctc tcccaaacgg gccccctctt
     1141 catacttctt tcctgcaggg gtgtggtgta accaggcagc acgacggttt ccaagtccga
     1201 cggttcgggt tcaaatcctg gcacccctgc cacacttcaa cctcaatttt ttgcggaggg
     1261 ttcgcctaac tggtaaggca ctcgtcttga aaacgagcgt gtttcgacac ctgagagttc
     1321 gactctctca ctctccgcca taaaattttc actcattatt caatattttt taccaggaaa
     1381 agtgtgtatt tttttggaaa ttttacatat tttttttcga attaattttg aatttatttt
     1441 tttattttta tcttttcttt acctggaggt gtcgcctaat ggtatggcag cggtctgcta
     1501 agtcgccaag ggcaaccaat acattgtgcc tgtgagggtt cgactccctc cgcctccgtt
     1561 ttttttgggt tcgtagctca gccggacgag agcacaggtt tgcgatgcct gaggtcggtg
     1621 gttcaagtcc acccgagccc tttttcaacc ctttgtgcgg gagtagcgaa atggcaaacg
     1681 cactggtctt aggaaccagg gactgtgggt tcgactccca ccttccgcac cacaaagttt
     1741 gaactcgtag ctcagccgga cgagagcaca ggtctccgac acctgaggtc ggcggtt
//
LOCUS       DAOQDO010001503         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68828_length_1796_cov_3.048248, whole genome shotgun sequence.
ACCESSION   DAOQDO010001503 DAOQDO010000000
VERSION     DAOQDO010001503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68828_length_1796_cov_3.048248"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..323)
                     /gene="cas2"
                     /locus_tag="PKZ53_20220"
     CDS             complement(30..323)
                     /gene="cas2"
                     /locus_tag="PKZ53_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNJ42819.1"
                     /translation="MDHSKHWYLVSYDLRDPKRWRKVYERVKGNAERVQYSVFKMYIN
                     QTQLERFRLDMAKLMDDEDDLLIIRLCSGCAKRVIDTSTDKSWDEARPPFQIL"
     gene            complement(355..>1796)
                     /gene="cas1"
                     /locus_tag="PKZ53_20225"
     CDS             complement(355..>1796)
                     /gene="cas1"
                     /locus_tag="PKZ53_20225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated endonuclease
                     Cas1"
                     /protein_id="HNJ42820.1"
                     /translation="WGIRGKVDAVRYRDGSLVAFEHKKGRSKSKEAWPWDRLQIIAYC
                     ALLEDATGKRIPEGRIRYHQDKTTVRIPFDDEARLELKTAIGRAQALRIQGERPPVTT
                     NSRACTHCSLAPVCLPEEERFVDEQQEHRPRRLFPENDDRRILHLTEPGTYVERSAEQ
                     LIAQLPDGRKVAFPGRDIAALVLHGPIQLTTQALHYCAIQEIGVHWLTGGGSYVGSLA
                     AGVPGVQRRHRQYRALSEPSFCLQLAIRVAHAKVENQLRYILRATRPSSGKEDERPAE
                     LETLIIRIRSALSHLTRATSVDELRGYEGQAGHAYFEALNFLIQPEAFKMKGRNRRPP
                     LDPTNALLSFGYALLYKECVAALLTVGLESAFGFYHTPRSAAQPLALDLMEIFRVLLV
                     DLPVIASINRKQWELREDFAVTPHKVWLNEDGRKKFLTLFENRKQETWKHPVLNYSLS
                     YARTIELEARLLEKEWTEQPGLFAKLRLR"
BASE COUNT          422 a          477 c          454 g          443 t
ORIGIN      
        1 gactcctgac aactgactac aaaccggtat tataaaatct gaaatggtgg tctggcttcg
       61 tcccatgatt tatccgttga ggtatcaatt actcgtttgg cacatcctga acaaagtcga
      121 atgatcaaca aatcatcttc gtcatccatc agtttcgcca tatccaggcg aaatcgctca
      181 agctgggttt ggttgatgta cattttaaag actgagtact gcacccgttc ggcgttacct
      241 ttgactcgtt catagacctt tcgccatcgt ttcggatcgc gaagatcata actcaccaga
      301 taccagtgtt ttgagtgatc catacttgtt ccctccagag attggttcag cccttcatcg
      361 aagccttaat tttgcgaaca accctggttg ttcagtccac tctttttcaa gcaaccgcgc
      421 ttcgagttca atcgtgcggg cgtaggaaag cgaataattc aacaccgggt gtttccaggt
      481 ttcctgtttt cgattttcaa acagggtgag aaatttcttc cggccatctt cattgagcca
      541 gactttgtgt ggcgtgacag caaaatcttc gcgaagttcc cactgtttcc gattgattga
      601 ggcaatgact ggcaggtcaa ccagcaaaac ccgaaagatc tccatcagat cgagcgccag
      661 aggttgggcc gctgagcgcg gagtatggta aaagccgaag gctgattcca gccctacggt
      721 cagcagtgcc gcaacgcatt ccttgtagag caacgcatac ccaaaactca acaatgcgtt
      781 ggtcggatca agcggtggac ggcgatttcg acctttcatt ttgaaagcct ctggctggat
      841 caaaaaattg agcgcctcaa agtaggcgtg cccggcctgg ccttcgtatc cgcgaagctc
      901 atcaaccgaa gttgcccgtg tgaggtgaga gagcgcactt cgaatccgaa taatcagggt
      961 ttccaactcc gctggacgtt cgtcttcttt tccagaggat gggcgagttg cccgcaaaat
     1021 ataccgaagc tgattttcca cctttgcatg ggcaacccgg atagccagtt ggaggcagaa
     1081 tgaaggttct gaaagggcac gatattgacg atgacgacgc tgaacacccg gtactccagc
     1141 cgccagactt ccgacatagc ttccaccgcc ggtcagccag tggacgccga tttcctgaat
     1201 tgcgcaataa tgaagcgcct gagtggtcaa ttgaattggc ccgtgaagca ccagtgcggc
     1261 aatgtcacga ccggggaagg caactttgcg gccatccggc aactgggcaa tcagttgttc
     1321 agccgagcgt tcaacatagg tacctggctc agtcagatga aggattcgcc ggtcatcgtt
     1381 ttccgggaaa agccggcggg gacggtgttc ctgttgttca tcaacaaaac gttcttcttc
     1441 cggcagacac accggcgcca gcgaacaatg ggtacaggca cgcgaattgg tggtcactgg
     1501 aggtcgttct ccctggattc gcaacgcctg cgctcgccca atggcggttt tcaattcaag
     1561 cctggcttca tcgtcaaacg ggatacgcac cgtggttttg tcctgatggt agcggatgcg
     1621 tccctcagga atccgttttc cggtcgcatc ctccaacaaa gcgcagtagg caatgatttg
     1681 caatcggtcc caaggccagg cttctttgga tttcgagcgg ccttttttgt gttcgaaggc
     1741 caccaaactt ccgtcacgat agcggacggc atcaactttt ccccgaatgc cccagg
//
LOCUS       DAOQDO010001504         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68863_length_1796_cov_2.095348, whole genome shotgun sequence.
ACCESSION   DAOQDO010001504 DAOQDO010000000
VERSION     DAOQDO010001504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68863_length_1796_cov_2.095348"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKZ53_20230"
     CDS             complement(<1..627)
                     /locus_tag="PKZ53_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42821.1"
                     /translation="MASLLKKFLFTATLTTVMLGAVGLFGTTAQAQTPESPVSLPIAS
                     DYTMACAGYIAAGPASEDIQIVGADREAEHDMYADGDIVYLNKGRGSNIQLGAEYQIV
                     RNIGPVKDFKGGKVVGNLVYELGLLRIVALQDNTATAQIKVACRPISFGDAAIPYDKK
                     VSPTPRPYKPLNLAGTPTGRTSGQILAAPDNREILGQNDVVYLNIGADN"
     gene            complement(858..>1796)
                     /gene="dnaJ"
                     /locus_tag="PKZ53_20235"
     CDS             complement(858..>1796)
                     /gene="dnaJ"
                     /locus_tag="PKZ53_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNJ42822.1"
                     /translation="DPQQRARYDRFGHAGVGTATAGGGQGFGDFTNIEDLLGDLFGFR
                     DVFNSGGRRSSSQVQRGADLRYDLEITLEEAANGVKPRVRIPRLETCETCTGSGAAAG
                     TSPSTCTTCSGTGQIRYQQGFFSVSRTCSACRGTGRMIKNLCKDCRGEGRVQKEKVLE
                     PKIPAGVDTGARIRIQGEGESGMGGGPAGDLYIVIHVKEHETFKRQDNNLYCTVPITI
                     SQAVLGAELKVNTLEGEETLSIPEGTQTDTVFRIRNKGIVSLSGRGKGDLFVSVTVVT
                     PSRLSREQRKLFEELAKLESQQAEEGFFDKVKNIFG"
BASE COUNT          441 a          475 c          440 g          440 t
ORIGIN      
        1 attgtcggca ccgatattga gataaacaac atcattttgg ccgagtattt cccggttatc
       61 cggggcagcc agaatctgac cagaggttcg cccggttggg gtccccgcaa gattgagtgg
      121 tttgtatgga cgtggggttg gggaaacctt tttgtcatag ggaatcgcgg catccccaaa
      181 actaattggc cgacaggcaa ctttgatttg ggcagtggcg gtattgtcct gtaaagccac
      241 aatgcgcaaa agacccagtt catataccag attgccaacg actttccccc ctttaaaatc
      301 cttcacaggg ccaatattgc gaacaatctg gtattcagcc ccaagctgaa tattgctccc
      361 acggcctttg ttgagataga cgatatcgcc atccgcatac atatcatgtt cagcttcccg
      421 atcagcccca acgatctgaa tatcttcaga cgctggtccg gcagcaatat atccggcaca
      481 ggccatcgtg taatctgatg caatcggcaa tgagactgga ctttctgggg tttgagcttg
      541 cgctgtggtc ccaaaaagcc ccactgcacc gagcatcacg gtggtaagag tggccgtgaa
      601 gagaaatttt ttcaacaagg atgccatgga cgttcctccg caaaagttga aggtctttca
      661 atgaagtggc aatgtgccga tgaaattgtg cgagaaaccc tggatgaaaa tgaaatggaa
      721 ggtgtgccta acttaccaga ctttgagtaa atccgtcaac gatggatttt tacagtccgg
      781 gaactgggga ttgattcctg gggtttggtg acaggtgatt ggagacggag agatatctca
      841 acaccagtca ccagagccta tccaaagata ttcttcacct tgtcaaaaaa cccttcttcg
      901 gcctgctgag attcgagttt cgccagttcc tcaaagagtt ttcgttgttc acgggacaga
      961 cggcttgggg ttaccacggt cacggaaaca aataaatcac cttttccacg accagagagt
     1021 gacacaatcc ctttattgcg aatccggaat acggtatcgg tttgcgtgcc ttccggaatg
     1081 gaaagcgttt cttcaccttc caacgtgttt accttcagtt cagccccgag tacggcctgt
     1141 gaaatggtaa tcggcaccgt gcaatacagg ttgttgtcct ggcgtttgaa ggtttcatgt
     1201 tctttgacat gaatgacaat atagaggtca cccgcaggac cgccgcccat tccagactcg
     1261 ccttctcctt gaattcgaat ccgagccccg gtgtcgaccc cagccggaat ttttggctcc
     1321 agaacctttt ctttttggac ccggccttcc ccacgacaat ctttgcacag gtttttgatc
     1381 atccgaccag tgccacggca ggccgaacat gtccggctga cactaaaaaa cccttgctga
     1441 taccggatct ggcccgtacc gctacaggtg gtacaggttg atggtgaagt acctgccgcc
     1501 gcacctgaac cagtacaggt ttcacaggtt tcgagtctcg gaatccggac acggggtttg
     1561 accccattgg cggcttcttc cagggtgatt tcaagatcat agcgaagatc ggccccacgc
     1621 tgaacctgac tgctggaacg gcggccaccg ctgttaaaca catcccgaaa cccaaataaa
     1681 tcgccaagca gatcttcaat attggtaaag tcaccaaatc cctgcccacc tccagccgtt
     1741 gcggtgccga cacccgcatg gccaaaacgg tcatatcggg cacgctgttg tggatc
//
LOCUS       DAOQDO010001505         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68898_length_1795_cov_3.657471, whole genome shotgun sequence.
ACCESSION   DAOQDO010001505 DAOQDO010000000
VERSION     DAOQDO010001505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68898_length_1795_cov_3.657471"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PKZ53_20240"
     CDS             complement(<1..1032)
                     /locus_tag="PKZ53_20240"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNJ42823.1"
                     /translation="MVSPRRKTLQTPAPGEVAGLSWRERWDIIHPWLGRSLWILGILA
                     LVWGTYRVIHSPAFTLAQITIQGQNRQSTRDIERLVQQTAPGSLLTASLTEIEQNIES
                     LPWIHHAQVTRILPNQVHIQVEERKPLLLVQGDQGNLVWIDEEGIPLEMYAPGKDQNP
                     PPIVIGLVAGNTEQNLRENLERIAVYKKLIWSLDSGTPQFSSKLESINLSRLEDVVVQ
                     LSDNPIVIHLGQEEFRDRLVRAMEVLDMVQRRDAQALEKYQIRDTGFLKYADQINSIT
                     AVNSAQISLGFRTDAGDSTEKSVNTSESASKSKNEAPTKPAASHSTSLEPVKPAKKEP
                     VKPAKKETKK"
     gene            complement(1065..>1795)
                     /gene="murB"
                     /locus_tag="PKZ53_20245"
     CDS             complement(1065..>1795)
                     /gene="murB"
                     /locus_tag="PKZ53_20245"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNJ42824.1"
                     /translation="GIPWSPLGYGTNLLVKDGPIRRVAVSLRALDDLLVYDGTRVHVH
                     AGYSLPRLVNGCADRGLAGIQGLAPIPGSVGGGVKMNAGSHGYEIADVIESVDVAREG
                     EVLRLKRDEIDFRYRYSPFTHQDLILGTILQLKPGNPADMKEEIKRYKQHRSATQPVR
                     ESSAGCIFKNPGSGLTTGRIIDELGMKGVFQGGATISTLHANFIVTDGKATAADIFAL
                     IDLIRDRVTTEHGLDLELEVEVWD"
BASE COUNT          389 a          471 c          454 g          481 t
ORIGIN      
        1 ttttttggtt tcttttttcg ctggtttcac tggctctttt ttcgctggtt tcactggctc
       61 cagcgaggtt gagtgactgg ctgccggttt ggtcggtgct tcgttttttg atttggaagc
      121 actctcactg gtgttaactg atttctcagt tgaatcgcca gcatcggttc gaaacccaag
      181 tgaaatctga gccgaattga ctgccgtgat ggaattgatt tgatcggcat actttaaaaa
      241 accagtatcc cgaatctgat atttctcaag cgcctgggca tcgcggcgtt gaaccatatc
      301 aaggacttcc atggcacgaa ccagccggtc ccgaaattct tcctgtccca aatgaattac
      361 aatcgggttg tcactgagtt gtaccacgac atcttcgagg cgactcaagt tgatactttc
      421 cagtttggaa gagaattgtg gggtaccgct gtcgagtgac cagatcagct ttttatacac
      481 cgcaatgcgt tcaaggtttt cccgtagatt ttgttcggtg ttgccggcca caagtccaat
      541 cacaatgggt ggcgggtttt gatctttgcc tggagcatac atttccagcg ggatcccttc
      601 ttcatcaatc cacaccagat ttccctggtc gccttgtacc aggaggagtg gttttcgttc
      661 ttcgacctgg atgtgtacct ggttgggaag aattcgggta acctgagcat gatgtatcca
      721 gggaaggctt tcgatatttt gctcaatttc agtcagtgat gcggtcagca ggctgcctgg
      781 cgccgtttgc tgaaccagcc gctcaatatc gcgtgtggat tgtcggtttt ggccctgaat
      841 tgtaatttga gcgagcgtaa aagccgggga gtgaatcacg cgataggtgc cccacaccag
      901 ggccagtatc ccaagtatcc agaggcttcg cccaagccag ggatggatga tgtcccagcg
      961 ttcgcgccag gacaggccgg ccacttcacc aggtgctggt gtctgaaggg ttttccggcg
     1021 gggtgaaacc acttgttcaa tttctctacc actgcgttgt gctgctagtc ccacacttcc
     1081 acctctagtt caaggtcaag tccgtgctcg gtggttaccc ggtcacgaat caggtcaatc
     1141 aaagcaaaaa tatcggctgc ggtggccttg ccgtcagtca caataaagtt ggcatgcagc
     1201 gtggatatgg ttgcgcctcc ctggaaaacc cctttcatcc caagttcgtc aatgatgcgc
     1261 ccggtggtca atccgcttcc tggattttta aaaatgcatc cagcactgct ttcgcggacc
     1321 ggttgagttg ccgagcgatg ttgcttatag cgcttgattt cttccttcat gtcagccgga
     1381 ttccccggtt ttaactggag aatcgtgcct aaaatcaaat cctggtgtgt gaacggtgaa
     1441 tagcgatagc gaaagtcaat ctcgtcacgt ttgaggcgaa gcacttcgcc ttcacgcgca
     1501 acatcaacac tttcaatcac atcagcaatt tcgtaaccgt gagaaccggc attcattttg
     1561 acgccgcccc ccacgctgcc tggaatgggc gccaacccct ggataccagc cagcccgcga
     1621 tctgcacatc cattgaccag tcgtggcagg ctatatccag catggacatg cacccgtgtg
     1681 ccatcataaa ccagcaagtc atcaagcgcc cgcagactga cagctacgcg ccgaattggt
     1741 ccatctttga cgagcaggtt agtgccatac ccaagtggac tccagggaat acctg
//
LOCUS       DAOQDO010001506         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68917_length_1795_cov_2.817816, whole genome shotgun sequence.
ACCESSION   DAOQDO010001506 DAOQDO010000000
VERSION     DAOQDO010001506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68917_length_1795_cov_2.817816"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PKZ53_20250"
     CDS             complement(<1..1496)
                     /locus_tag="PKZ53_20250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ42825.1"
                     /translation="MAHSPGSFIFRYSVAIISVALAIWARLLFDPVLGLQFPFATVFL
                     AVLITAWYGGFRPALVALVLGAIGSRYFLIPPRGSLGMTSFEQQMGLALYIFTGLGIS
                     LLAGSMHAARVEAESTAESVRRQAALIDQTYDAVLVWDWNGPITFWNRGAERLYGYSY
                     QEVLGRLSHELLHTERQGGLIELRNQLEKEGFWEGELKQTTRDGQQITVETRMVLVRR
                     AGEVYVLETDRDITRRKHIEAELLAAKDQLEIRVDQRTAELTQAHELLQASEERFRLL
                     IEGVKEYAIFMLDPAGHVMTWNSGAERHTGYQAEEIIGRHFACFYCEDDIAKARPEYT
                     LEKVLADGRCEDEGWRVRKDGSRFWARVVITPLYDTSGRLRGFAKVACDITEQKMKDE
                     ALQDRERLLSIVTDSARVGLVVVGPGYRYLFANKAYAEILGLETHEIVGRRVPEMLAP
                     AWSQIQPRLDRAFSGEQVTYELMMPPLPGNQMVRYFTVVYEPGITDSG"
BASE COUNT          462 a          495 c          433 g          405 t
ORIGIN      
        1 aatccgctgt ccgttatccc tggttcgtac accacagtaa aataccgaac catctggttg
       61 cctggtagtg ggggcatcat cagttcatag gtaacctgtt caccagaaaa tgcgcggtcc
      121 agccggggct ggatctgaga ccaggctgga gccagcatct caggaactcg acgcccgaca
      181 atctcgtggg tttcgaggcc caaaatttca gcataggctt tgttggcaaa gagataccgg
      241 tatccaggac ccaccaccac caacccaacc cgtgctgaat cagtcacgat gctgagaagc
      301 cgctcgcgat cctgtaacgc ctcatccttc attttttgct cggtgatatc gcaggcaact
      361 ttagcaaagc cacggaggcg gcctgatgtg tcatataaag gagttatgac cactctcgcc
      421 caaaagcgtg aaccgtcttt tcggactcgc catccttcgt cttcacaccg accatcagcc
      481 agaaccttct ctagcgtata ctcaggtctg gctttggcaa tatcgtcctc acaataaaaa
      541 caggcgaaat gccgtccaat aatttcctca gcctggtatc cggtatggcg ttctgcacca
      601 gaattccacg tcatgacatg acctgccgga tccaacataa aaatggcgta ttctttcacg
      661 ccctcaatca agagtcgaaa ccgttcttct gaagcctgta acaattcatg ggcctgggtt
      721 aactcagccg tgcgctggtc aacgcggatt tcaagctggt ctttggctgc cagtagctca
      781 gcttcaatgt gtttgcggcg ggtgatatca cggtcggttt caagcacata aacctcaccc
      841 gccctgcgaa ccagcaccat tcgggtttcc acggttatct gctgtccatc acgagtggtt
      901 tgtttgagtt cgccttccca aaacccttct ttctcaagct ggttgcgtaa ctcgatgagt
      961 ccgccttgtc gttcagtgtg caagagttca tggctgagtc gtcccaatac ttcctggtag
     1021 gaataaccgt acagtcgttc cgcaccgcga ttccaaaacg taattggccc attccagtcc
     1081 cagaccagaa cagcatcata ggtctggtca atgagtgccg cctgacgtcg aaccgactca
     1141 gccgtggact cagcctcaac tcgtgccgcg tgcattgacc cggcaagtag cgaaatcccc
     1201 aaaccggtga aaatatagag agccagcccc atttgttgct caaagctggt cattcccaaa
     1261 ctcccgcgtg gggggatcaa aaaatatctt gacccaattg ctccgagcac aagtgccacc
     1321 agggctggtc gaaaaccgcc ataccaggca gttatcagca cggccaggaa aaccgttgca
     1381 aatggaaact ggagcccaag taccggatca aataaaagtc tggcccaaat cgccagagct
     1441 acactgatga tggcgacgct ataacggaag ataaacgagc cgggggaatg agccataaga
     1501 cgtcctcatt gctcgataga gacagctcaa gagtagaagc gaaaaccggt caaccccaaa
     1561 atttcagggt caggcaggta cctgggagca gatcaacaga ggttgaaaac agcagccgaa
     1621 ttttcaaggc cgagtctttg aaaagagtca ttctgtcgct tcactctaca ccgaggcccg
     1681 gcgcaaaact accccatgca gccttttttg atcaataaag ctaaatctca ttttatctct
     1741 ttgagttccc aaatatggat ggggaatggg ggatggggga taggggatgg gggat
//
LOCUS       DAOQDO010001507         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_68999_length_1794_cov_2.459459, whole genome shotgun sequence.
ACCESSION   DAOQDO010001507 DAOQDO010000000
VERSION     DAOQDO010001507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68999_length_1794_cov_2.459459"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKZ53_20255"
     CDS             <1..180
                     /locus_tag="PKZ53_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ42826.1"
                     /translation="RVLSRTVLSEKVWDLSFDTLTNVIDVYINYLRNKIDRDFEPKLI
                     HTIRGAGYMLKEPGN"
     gene            687..1637
                     /locus_tag="PKZ53_20260"
     CDS             687..1637
                     /locus_tag="PKZ53_20260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42827.1"
                     /translation="MRFCPEDGTRLPDLAGEDYLLGRTLDNKYLIKSKIGQGASGAVY
                     RARRLSIQDDVAVKVLKPELVADSAATERFRREALALGRIHHPNTIAVYDFGVTSDGI
                     TFLVMEMLSGRTLRQVLREEKMLDLHRTVHLFNQMCSALNVAHNNKIIHRDLKPENII
                     VEKYEGQGEVVKVIDFGLAKLRFTGNLMKTLTEQGRVAGTPYYMSPEQWMDKTIDART
                     DVYGLGVILYEMLTGCVPFNAPTVMQLANKHVRIPPQPPNTLRPDLPKPVSDVVLRAL
                     SKKPDDRPSSTLEFAQELTEAADCSLPPNLAGRNFPLRNV"
BASE COUNT          410 a          447 c          465 g          472 t
ORIGIN      
        1 cgagtgcttt cgcgcacagt tttgtctgaa aaagtctggg atctgtcgtt tgacaccctg
       61 accaacgtca ttgatgttta tatcaactac ttacggaaca aaattgaccg cgactttgag
      121 ccgaagttaa ttcataccat tcgtggtgcg ggatatatgc tcaaggaacc tgggaattag
      181 ggctgaagac attgggctgg gggctgaggg cggaagaagt cgggctgaag acaatcgggc
      241 tcagggcaga agacattggg ctgaagactc gcaagctcgg ggcggaagac aacgggttta
      301 atctcccgaa cccaatttct tcaacccaac ttcttcagcc cgacttcttc agccccaagc
      361 cctaagccca atgtcttccg cccaaggtct tcaacccttt ttcttgaggc ttttgcattc
      421 ccggattggc gctttttggt gaaacgtcgt atactgcccg ggtctgttca tccacaatct
      481 ctacgctccg aaaaataatc aattcaatcg cttgcacgtg ccaatcccct cattgccacg
      541 gttggccaga gggaacaagc tgtcaaagag gatactccag ggaacttcct ccacctggca
      601 ggaataactc ggtcaggttg gccttgtctc acccttgatt gcctatgaaa aaatgcccac
      661 gttgtacccg agattggcca gataccatgc gattttgccc agaggacggg actcgattac
      721 cggatctggc cggtgaagat tatttattgg gtcgaacgct tgataacaaa tacctgatca
      781 aatccaaaat tggtcaaggc gccagtggcg ccgtctaccg tgcccggcgg ttgtcaattc
      841 aggatgatgt ggcggtcaag gtactaaaac ccgaactcgt agccgactcg gcagcaactg
      901 agcgttttcg acgtgaagcg cttgcccttg ggcgaatcca ccaccccaac accattgcgg
      961 tgtatgactt tggcgtaaca tcagatggta ttacgttcct ggtgatggaa atgctgtctg
     1021 ggcgaacctt acgacaggtc ctgcgcgaag agaagatgct tgacctgcat cggacagttc
     1081 atctgttcaa tcagatgtgt tcagcactca atgtcgccca taacaacaaa atcattcatc
     1141 gtgatctgaa accggaaaat atcattgttg agaaatatga gggtcagggt gaagttgtca
     1201 aagtaattga cttcggcctg gcgaaactcc ggtttaccgg taacttgatg aagacgttga
     1261 ccgagcaggg acgcgtggca ggaacaccat attatatgtc cccagaacaa tggatggata
     1321 aaaccattga tgctcgcact gatgtttatg gtctcggggt aattttgtat gaaatgctga
     1381 ccgggtgcgt tccctttaat gccccaacgg tgatgcaact ggccaacaaa cacgtccgga
     1441 ttccacctca accccccaac acgcttcgac cagacttgcc caaaccggtc tcggacgtgg
     1501 tgttgcgggc cctgagcaaa aaacctgatg atcggccttc gtcaacactt gagtttgccc
     1561 aggagttaac cgaagccgct gattgttcac tgccgccaaa cctggcaggt cgcaattttc
     1621 cgttgcgcaa tgtttaaggt gcttggttcc tggttcttgg tgcttggttc ctggttcttg
     1681 gttcctggtg cttggttctt ggttcctggt tcttggttcc tggtgcttgg tgcttggtgc
     1741 ttggtgcttg gtgcttggtt cttggttctt ggtgcttggt gcttggttct tggt
//
LOCUS       DAOQDO010001508         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69044_length_1793_cov_3.668585, whole genome shotgun sequence.
ACCESSION   DAOQDO010001508 DAOQDO010000000
VERSION     DAOQDO010001508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69044_length_1793_cov_3.668585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKZ53_20265"
     CDS             <1..718
                     /locus_tag="PKZ53_20265"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNJ42828.1"
                     /translation="IKHELKDKISTMCGDCQRRYETNPLRILDCKIDTAVVAQLPSTI
                     NLLSDESRRHFEALCRMLDERGISYVVDSQMVRGLDYYTKTVFEIVSGSLGSQSALLG
                     GGRYDGLSETLGGPPAKGFGFAIGVDRFAMSLPEDVKDTNTPQVFVAPLGEAAFHKAV
                     TIVSELRRAGVATLMDFEHRSLKSSMKLADKVGAQLVLILGETELQTGQWALRSMKDS
                     SQTTISAENWLSGIQKALNC"
     gene            805..>1793
                     /gene="pta"
                     /locus_tag="PKZ53_20270"
     CDS             805..>1793
                     /gene="pta"
                     /locus_tag="PKZ53_20270"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNJ42829.1"
                     /translation="MKILETLRLRAAAKPQHIVLPEGEDERTILAAQMVDRLKMARLT
                     LIGNAEKITEKARSLGVDLGPFTVVDPRKTPDLELYAREYYDHRKAKGIAPDEARKQV
                     MEPLYFGNMMVRLGKADGSVAGATNTTAHTVRAALQVIGLHPKFKIVSSFFLMALPNS
                     SLGASGNFIYADCGVVINPNATELAEIAIASAESCRALLQVEPRVAMLSFSTKGSASH
                     PLVDKVVEATRTVRARAPELSVDGELQADAALIADIGSKKAPGSTVAGKANVLIFPNL
                     EAGNIGYKLTERLAGATAIGPILQGLAKPANDLSRGCKAEDIVDAIAITAVQA"
BASE COUNT          446 a          469 c          492 g          386 t
ORIGIN      
        1 gatcaaacac gaactcaaag ataagatcag cactatgtgc ggagactgtc agcgacggta
       61 cgaaaccaac ccgctgcgca ttctggattg caaaattgac acagcggtgg tcgcccaact
      121 gccatcaacc atcaacttgt tgtctgacga aagccgccgt cactttgagg cgctgtgccg
      181 gatgcttgac gaacgcggaa tttcctacgt ggttgactca cagatggtac gcgggttgga
      241 ttactacacc aaaaccgtgt ttgaaatcgt ctcgggctcg cttggatcac agagtgcctt
      301 gcttggcggc ggacgctatg acggactatc tgaaacactc ggtggtccac cagctaaagg
      361 attcggattt gccattggcg ttgaccggtt tgcgatgtcg ctgcctgaag acgtcaaaga
      421 caccaacacg ccacaggtat ttgtggcccc actcggggaa gccgcatttc acaaagccgt
      481 cacaattgtc agcgaacttc gacgggctgg cgtggcgacg ttgatggatt tcgaacaccg
      541 aagtctcaaa agctcaatga aactggcaga taaagtcggc gcccaactgg ttttgatttt
      601 gggtgaaacc gaactccaaa ccggccagtg ggcgttgcgc tcaatgaagg atagctcaca
      661 aaccaccatt tcggctgaaa actggctttc tggtatccag aaggccctta actgttagcc
      721 tttggtgcct ggtgcttagg aaccctatag tttcaaagca ccaggcacca ggtcccaagt
      781 cccaaacacc aggtcctaaa acgtatgaaa atccttgaaa cccttcgact ccgggctgcc
      841 gccaaaccgc aacacattgt cctccctgaa ggtgaagacg aacgcacgat tctggcagca
      901 caaatggttg atcgtttgaa aatggcgcgg ctgacattga ttggcaatgc ggaaaagatc
      961 acggaaaagg cccgttcgct gggtgtggat ttgggtccgt ttaccgtggt tgatccgcgt
     1021 aaaacaccag acctggaact ctatgcccgt gaatattatg accaccgcaa agccaaaggg
     1081 attgctccgg atgaagctcg caagcaggtg atggagccac tctatttcgg caacatgatg
     1141 gtgcgactcg ggaaagcaga cggttcagtg gctggcgcga ccaataccac ggcccacacc
     1201 gtacgggctg cactccaggt gattgggctt catccaaagt tcaagattgt gtcgagcttt
     1261 tttctgatgg ccttgccaaa ttcatcgctt ggggccagcg gaaacttcat ttatgccgat
     1321 tgcggtgtgg tgatcaaccc aaatgccacc gaactggccg agattgcaat tgcgtcagcg
     1381 gaaagctgcc gggcacttct gcaggttgaa ccacgggtgg cgatgctctc attttcaacc
     1441 aagggcagtg cgtcgcatcc actggtggat aaagtcgtcg aagccacccg aaccgttcgg
     1501 gcgcgggcac cagagctttc agtggatggt gaattgcagg cggatgcggc gttgattgcc
     1561 gatatcggct ctaaaaaagc accaggaagt acggtggcag ggaaggccaa cgtgttgatt
     1621 ttcccaaatt tggaagcggg caacattggc tacaaactga ccgaacgact ggccggcgcc
     1681 accgcgattg gtccgatttt gcaagggctg gccaaaccag ccaatgacct gtcacgtggc
     1741 tgcaaagctg aagacatcgt ggatgccatt gccattacag cggttcaggc aca
//
LOCUS       DAOQDO010001509         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69252_length_1790_cov_5.339481, whole genome shotgun sequence.
ACCESSION   DAOQDO010001509 DAOQDO010000000
VERSION     DAOQDO010001509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69252_length_1790_cov_5.339481"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1248
                     /locus_tag="PKZ53_20275"
     CDS             298..1248
                     /locus_tag="PKZ53_20275"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNJ42830.1"
                     /translation="MSSAPIAPSSVSGNPLEVEIAGIRFKNPVLPASGTFGYGLEFLP
                     FFDVSRLGGFCTKGLSPKPLKGNPPPRIAETSAGMLNAIGLQNVGVKVFIEEKLPELR
                     AYDTQIIANVFGYSIDDYLEVVQMLNDAEGVAAYELNISCPNVKQGGTQFGNDPVLAA
                     EVTSAVKKAATRPVIVKLSPNAGDLIGVARAVEDAGADAVSLINTIVGMSIDVYTHRP
                     RLAFATGGLSGPAIKPIAVRMVYEVSKVLRIPRIGIGGIVSAMDAIEFILAGATAVQI
                     GTANYADPCAALRLVEGLELYCQEQKVTRITDLVGAVKTK"
     gene            1432..>1790
                     /locus_tag="PKZ53_20280"
     CDS             1432..>1790
                     /locus_tag="PKZ53_20280"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ42831.1"
                     /translation="MSNQSAPLTKTVVVIGGGFAGLAAATALAEAGYQVQVIERRPFL
                     GGRAYSMRDAKTGDVIDNGQHLMMGCYRETFAFLRRIGSFDLVEFQTQSRVDFLDRDG
                     HQTLECPNLPAPLHLLAG"
BASE COUNT          452 a          414 c          480 g          444 t
ORIGIN      
        1 ctggtggcag attggttatt gcgggggctg ggaacgacgc agttggttgg gattcagcgg
       61 caagggcagg cgcaaccgac tctggaactg tagtagccga agcagacgaa cgtccattca
      121 taaaattcag aaaatcaaag cgttatggtt cttgtttatc catttatgtg ccaggaagtt
      181 gataaatgac ttcccaagtg ggaggtcaat cagtacactg gccagtttca atccatccgg
      241 tacggatcaa agggttcttt tcctcatcaa acccgaaaaa accgagggta tcattttatg
      301 tcgtctgcgc ctattgctcc ttccagtgtg tctggcaatc cacttgaggt cgaaattgcg
      361 gggattcgct ttaaaaatcc ggtgctgccc gccagcggaa cctttggcta tggactggaa
      421 ttccttccat tttttgatgt atcgcgatta ggtggctttt gtaccaaggg gctttcccca
      481 aaaccactca aaggaaatcc gccaccacgc attgccgaga ccagcgccgg catgctcaat
      541 gcaattggtt tgcaaaatgt tggtgtgaaa gtatttatcg aagaaaaact ccctgagctt
      601 cgggcctatg acactcagat tattgccaat gtgtttggct attcgattga tgattatctc
      661 gaagtcgtcc agatgctcaa cgatgctgaa ggcgtggcag cctatgaact caacatttcc
      721 tgccccaacg tcaaacaggg aggcactcag tttgggaatg atccagtttt ggcggctgaa
      781 gtcacgtcag cggtcaaaaa agccgctacg cgtccagtca ttgttaaact ttcccccaat
      841 gctggcgatt tgattggtgt ggcacgagcg gtcgaagatg ccggtgcgga tgcggtttca
      901 ctgatcaaca ccattgtcgg aatgtcaatt gatgtttaca cccatcgtcc acggctggca
      961 tttgccacgg gtggcctctc tgggccggct atcaaaccga ttgcggtgcg catggtttat
     1021 gaggtgtcaa aggttctgcg catcccccgg attggcatcg gtgggattgt ctccgctatg
     1081 gatgcgattg aatttattct ggctggcgcg accgccgtgc aaattggaac cgccaattat
     1141 gctgatccct gtgccgcact tagactggtc gaaggtctgg aactgtattg tcaggaacaa
     1201 aaagttaccc gaatcacaga tctggtcggc gccgtgaaaa ccaaatgaga atgaagaatg
     1261 aagaattgag aatgaagaat tgagaatgaa gaattgagaa tgaagaattg agaatgaaga
     1321 atgaagaaag tacctggtac ttagtgctta ggaaccaata ctttccaaga accaagaacc
     1381 aagaaccaag aaccaggaac caggaaccag gaaccagtag ccctaatccg aatgagtaat
     1441 caatctgcac ccctcacaaa aacagtcgtc gtcattggcg gaggctttgc cgggctggcc
     1501 gccgccaccg cactggctga ggctggatat caggttcagg ttattgaacg tcgcccattt
     1561 ttgggtggac gggcctactc gatgcgcgat gcaaagactg gcgatgtgat tgataacggg
     1621 cagcatctga tgatgggttg ttatcgtgag acgtttgcgt ttttgcgccg aattgggagt
     1681 tttgatctgg tggaattcca aacccagtca cgggtagatt ttctggatcg ggatggtcat
     1741 cagaccctgg aatgcccaaa cctcccggca ccactgcatc tgctggcggg
//
LOCUS       DAOQDO010001510         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69428_length_1788_cov_2.941143, whole genome shotgun sequence.
ACCESSION   DAOQDO010001510 DAOQDO010000000
VERSION     DAOQDO010001510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69428_length_1788_cov_2.941143"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..520)
                     /locus_tag="PKZ53_20285"
     CDS             complement(32..520)
                     /locus_tag="PKZ53_20285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ42832.1"
                     /translation="MSRPFRYFLRVRFHECDAQKVVFNAIYGTYVDIAVLEFLRAIGL
                     GEAVTGQFDYQLVKQTVEWKAPARFDQVLEISVWAKHLGNTSFTLATEFRRAGQPHIN
                     VTAETVYVLVDAQTLSKTPLPPHFRHTLEAGGWGLWVDHASCTVHRAESLESQPPENQ
                     KN"
     gene            complement(626..>1788)
                     /locus_tag="PKZ53_20290"
     CDS             complement(626..>1788)
                     /locus_tag="PKZ53_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42833.1"
                     /translation="EEGQVKTPVVSLAQQTNVPGTPNSAVDKFSFIGDLEDESNVTYY
                     IDDVSVSLDKPVTQLSFVAPGRRMLFVDRWNNYYQAIRQRPTWIPAIDFSDFGISSAT
                     VKQMKQEGSYKYLEGLLAGQCLQAEAEKVASPENFRLLKAVCLWNQGCGAMSAGRPKT
                     ALERYDQAAQLSPGGKIYGLSAVLALSALKQWDEVNARLGSVYTNFQTDIRLSVALAM
                     IGTARGNLDEAQQWLRIPAETLTNDFRNTLLRRLWGAEISSDVVSELKSKFPDDWREI
                     LQTRLICEQYFFVLLWKNNYTEAERYASRMAERMKLLGLPEWHWHERAGDAAFLAGNP
                     AGALPHYEAALKDPAGRYVMLRMSDAYFRLGDFAKERDYREKIYGSLLQKTN"
BASE COUNT          438 a          490 c          423 g          437 t
ORIGIN      
        1 agcagaaatt gggcgtaact cattgcgatt cctaattttt ttgattttcg ggcggttgag
       61 actccagaga ttctgcacga tgaaccgtac aggaggcatg atcaacccag aggccccaac
      121 ctccagcttc aagcgtatgg cgaaagtgag ggggcagcgg cgttttggaa agcgtctggg
      181 catcaaccag gacatacacc gtttcagccg tcacattgat gtggggttgc ccggctcgcc
      241 gaaattcagt cgcgagtgta aacgatgtat ttccaaggtg ttttgcccaa accgaaattt
      301 caagtacctg atcaaaccgg gccggggcct tccattcaac cgtttgtttg acaagctgat
      361 aatcaaattg tccggttaca gcttcaccaa gcccaattgc ccgcaggaat tcgagcaccg
      421 caatatccac ataggtaccg taaatcgcat taaaaactac tttctgcgca tcgcattcat
      481 gaaatcggac acgcagaaag taccgaaacg gacgtgacat gagcgggttc tccagggttg
      541 aaatcagcca aaaacccaat cctgtttgag aaaccagcgc gggtcaaccc ttcttcgagg
      601 agaaatgatc aaccacacct cacattcagt tcgttttctg aagcagactg ccatagatct
      661 tttcccgata gtcacgttct ttggcaaaat cacccagccg gaaataggca tctgacattc
      721 gcaacatcac atagcggcca gccgggtctt ttaatgccgc ttcgtaatga ggaagcgctc
      781 ctgccgggtt tccagccaga aaagcagcgt ctccggcgcg ttcgtgccag tgccactctg
      841 gcagtcccag cagtttcatc cgttcggcca ttcgtgaggc ataccgttcc gcttcggtgt
      901 aattgttttt ccacaacaac acaaagaaat actgctcaca aatcagccgc gtttgtaata
      961 tttcgcgcca gtcatccggg aacttactct tcagttcgct gaccacgtcc gaactgattt
     1021 ccgctcccca taaccgtcga agcagggtgt ttcgaaaatc atttgtcagg gtctccgctg
     1081 gaattcgaag ccattgttgt gcttcgtcaa ggtttccgcg agcagtgcca atcatggcca
     1141 gcgccaccga taatcggatg tctgtctgga aattcgtata cacgctccca agccgggcgt
     1201 tgacttcatc ccattgtttc aaagcggaga gcgccagcac tgccgacaat ccgtagattt
     1261 tcccgcctgg cgataattgc gccgcctggt catagcgttc aagcgccgtt ttaggccgtc
     1321 cagccgacat ggcgccacag ccctgattcc acaaacacac ggctttgagc aaccggaaat
     1381 tttcgggtga agcgactttt tcagcttcag cttgcaaaca ttggccagcc agcaagcctt
     1441 caagatactt gtagctgcct tcctgtttca tttgtttgac cgtcgccgat gaaatcccga
     1501 aatctgagaa gtcaatcgct ggaatccacg ttggacgctg acggatggct tgataatagt
     1561 tgttccaccg atccacaaag agcatccgtc gccctggggc cacaaatgaa agctgggtca
     1621 ccggtttatc aaggctcacg ctcacatcgt caatgtaata ggtgacattt gattcatctt
     1681 ccaaatcacc aatgaaggaa aatttgtcta cggcggaatt gggtgtaccc ggcacgttgg
     1741 tttgctgggc gagcgacacc actggcgttt ttacctgtcc ttcctcaa
//
LOCUS       DAOQDO010001511         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69582_length_1786_cov_3.002889, whole genome shotgun sequence.
ACCESSION   DAOQDO010001511 DAOQDO010000000
VERSION     DAOQDO010001511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69582_length_1786_cov_3.002889"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(432..1244)
                     /locus_tag="PKZ53_20295"
     CDS             complement(432..1244)
                     /locus_tag="PKZ53_20295"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNJ42834.1"
                     /translation="MEKAPVMLPILPSYSNSYHPSSDWASQLVAKQALWQTRQTAGHP
                     VIEPVTTWTLNEWVVTELQFDAPHLDREQVLAWLTVTDQSQLAEPIQHEAVNLFRAVN
                     AIAQRAKTLPATERDALGKEDLIHSYRLLTGNPVSEWNELFRRTEAVPQVPTHQPAAP
                     HALPILVEMALDWFTTDSFAEMHPVEKASLFHLRMLDLQPFASHTNRLVRLLTSYYLL
                     RDGLPPLMIAAHNQQAYFDTLGFSFQMITQPGVELFARLLEQTLDQWLEMTK"
     gene            1545..>1786
                     /locus_tag="PKZ53_20300"
     CDS             1545..>1786
                     /locus_tag="PKZ53_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ42835.1"
                     /translation="MATRLRQYLLIWFCMFLAWPGLSVPAQTRASRPAPAKSAGSPKL
                     ILLIVVDQFRPDYLTRFGDLFGAGGFRRLLNQGAYFT"
BASE COUNT          441 a          452 c          450 g          443 t
ORIGIN      
        1 gcagttgact gtttggtttc agcggtatca gcggcaatat tggcggcaag cgggaaaccc
       61 gccaccaaca taccgaccat aaaaaagacg attcctctcg tcaaaagacg ctgcatagac
      121 atactctcct catcttcaag cttagaagtc ccctgggaaa tgagctttga tttccttctt
      181 cactctctgt tctaaaatcg catccagcac gcgcatacgg cacaaagatc ggcctcaaaa
      241 atatgtgctg aacccctggg attggttcac atctgcaatt ttctatggtg agagtgagcg
      301 aaggtttttt ggaagggtca atattagagg acttgaggcg aaatgcaaag ctgaccggat
      361 gacaaagtga ccaggtgacc tctgacacag tgccagaatg atctatttta tcctgctccc
      421 ctgtcatcct gtcatttggt catttccagc cactggtcca aggtttgctc taaaagtcgg
      481 gcaaagagtt ccacgccagg ttgagtgatc atctgaaatg aaaaaccgag cgtgtcaaag
      541 taggcctgct gattgtgagc agcaatcatc aagggcggta atccgtcacg caggagatag
      601 taactggtca gcaatcgcac cagccggttg gtatgacttg caaatggctg caaatccaac
      661 atccgcaaat gaaagaggct ggccttttca accgggtgca tttcggcaaa cgagtcggtg
      721 gtaaaccagt caagcgccat ttcaaccaga atcggtaagg cgtgaggcgc cgctggttga
      781 tgggttggaa cctgggggac agcctcagtc cggcgaaaaa gctcgttcca ctctgacact
      841 ggattgccag tcaatagccg gtaactatgg atcagatctt cttttcccaa ggcatcccgc
      901 tcagttgccg gaagtgtttt ggcgcgttgg gcaatggcgt tcaccgcacg gaacaaattc
      961 actgcttcgt gctgaatcgg ttctgcaagt tgggattggt ccgtcacggt cagccaggcc
     1021 agtacctgct cacggtccag atgtggagcg tcaaactgga gttcagttac aacccattcg
     1081 tttaaggtcc aggtcgtcac aggttcaatc acgggatggc cagccgtctg gcgggtctgc
     1141 cagagtgctt gtttggccac gagctggctg gcccagtcag aggaaggatg ataggagttg
     1201 ctataactcg gcaaaatcgg aagcataacc ggggctttct ccataaatga ttttcctgat
     1261 ttaagactga aatgagaaaa tcaccaattc taacttccgg caaatgagag ttcagcggtc
     1321 aggggtttcg aaaccagtcg ccagtcatca gatacaaaac acgaacaagg tggacggatc
     1381 ccgttgtttt ccgatagagt aggtaacctg tttcccaaac aggctttccg cgctcaaaat
     1441 tcccgatgcc caacctattt ttccactgag ctaaaaccgg catgatcatt tcatcttccc
     1501 cctctgaatc acctgtacct gtcgttcagt ctcgtggtat cacgatggca acacgccttc
     1561 ggcaatatct tctcatctgg ttttgtatgt ttctggcctg gccaggactg agtgttccag
     1621 cacaaacacg ggccagtcgt ccggcaccag ccaaatcagc cggctcgcct aaactgattc
     1681 tcttaattgt ggttgatcaa tttcgccctg attacctgac ccggtttgga gacctgtttg
     1741 gggcaggtgg atttcgacgc ttgctcaatc agggggcgta ctttac
//
LOCUS       DAOQDO010001512         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69583_length_1786_cov_2.964760, whole genome shotgun sequence.
ACCESSION   DAOQDO010001512 DAOQDO010000000
VERSION     DAOQDO010001512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69583_length_1786_cov_2.964760"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKZ53_20305"
     CDS             complement(<1..446)
                     /locus_tag="PKZ53_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42836.1"
                     /translation="MSELLASDTLIAVASITESVVGNIKLPGSVNTVEVKNQLAYGAS
                     VVMDQMTCLLDSLTRTFGPPIVIPVPGSTPLRGLNVLDMNQDDLVDVIAIAEDGDNQR
                     RLLVFPRTSATTFGTPIESEIITRDLILAVGTVDFVYSGTDLLVLG"
     gene            complement(582..>1786)
                     /locus_tag="PKZ53_20310"
     CDS             complement(582..>1786)
                     /locus_tag="PKZ53_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42837.1"
                     /translation="IDAQTTIIQTIAGNGTKGEAGVPGEQLATQTGLGILYGGGIFVS
                     ASSQLVITEGQWDRIRRVDLRTGMISVLAGGKPSDNGDKGPALTAKLVFSDIVNRLGI
                     VKPGEIAVTESGETIFLAEPARHRVRKIDLTTGFITTIAGTGISGYNGDSQPAEKAML
                     SEPTDVAISPGGNIMIADNRNHRIRQIEREDGLIRTVAGNGKGELNADYDEDYNEFYF
                     LDDDGPALEAAMVDPLGIALDANGNLFLSLFGVFVRRVDAQTQLVTTPIGCCFGSGRR
                     FLRGITVNPAGVVYVANTNQGILRLGPGETQPSAWFFGTSFFDVVTNRDGVVFGAALT
                     ENAIYQIEQVFGIKIAGGKLGYEGDNGPAREAAFSSPSAVAIDGANNLYIVDSGNNAV
                     RVIKNGAR"
BASE COUNT          446 a          514 c          393 g          433 t
ORIGIN      
        1 tctccgagca ccagcaggtc agttccgctg tagacaaaat ccacggttcc aaccgcaagg
       61 attaaatcgc gagtgatgat ttccgattca atcggagtcc caaacgtcgt ggccgacgtc
      121 cgggggaaca ccaaaagtcg gcgctggttg tctccatctt cggcaatcgc gatgacgtca
      181 accaggtcat cctggttcat atcaagcacg ttcaaccctc ggagcggggt tgaacctgga
      241 accggaatca caatcggcgg accaaaggtc ctggtcaggg aatccaataa acaggtcatc
      301 tgatccatca ccactgaagc tccataggcc aactgatttt tgacttcgac agtattgact
      361 gagccgggaa gtttgatatt gccaaccacc gattcggtga tggaggccac ggcaataagc
      421 gtatcggagg ccaatagttc cgacacgacg tgcgattcat aagttccctt tttaagcctt
      481 tttcaaatca tcgctttgcc tcagcaacag gcgaaatgat tgaaaaaatg gtgtttccct
      541 gatcctgaat ctgaaatggt gttgcttcac aaccacggta tttatcgggc gccgtttttg
      601 atcacccgaa ccgcattgtt tcctgaatcc acaatgtaaa gattatttgc cccatcaatc
      661 gccaccgcag atggagacga aaaggccgct tccctggctg gcccattgtc gccttcgtag
      721 ccgagcttgc caccagcgat tttaattcca aagacttgct caatttggta aatcgcgttt
      781 tcagtcaggg cagctccaaa caccacccca tcacggttgg tcaccacatc aaagaacgag
      841 gtaccaaaaa accaggcgct cggttgagtc tctcctggtc ccaacctcaa gattccttga
      901 ttggtgttgg cgacataaac caccccggct gggttgaccg taatcccacg caaaaatctt
      961 ctcccgctcc caaaacaaca cccaattggg gtcgtgacga gttgggtttg agcatcaacc
     1021 cgacgcacaa acactccaaa gagggaaaga aacagattgc catttgcatc cagagcaatc
     1081 ccaagtgggt caaccatggc cgcttccaaa gcgggaccat cgtcatccag aaagtaaaac
     1141 tcattgtaat cctcatcata atcggcattc agttcaccct tcccgttccc cgcaaccgtg
     1201 cgaatcaatc catcttcacg ctcaatctgg cgaatccgat gatttcgatt atctgcaatc
     1261 ataatatttc cgcctgggct aatcgccaca tctgtgggtt ccgacagcat cgctttttcc
     1321 gctggttggc tatccccgtt gtatccagag atacctgttc cggcaatggt ggtaatgaac
     1381 ccggttgtca gatcaatctt tcgcactcgg tgccgggcgg gctccgccag aaatatggtt
     1441 tccccgctct cagtcacggc aatctctcct ggtttgacaa ttcccagccg attcacaata
     1501 tctgagaaca ccaactttgc ggtgagggct ggtcccttgt ctccattgtc tgatggtttc
     1561 cccccagcca acactgaaat cattcccgtt cgtagatcaa ccctgcgaat ccgatcccat
     1621 tgcccctcgg taatcactaa ttgagaagaa gcactgacaa aaattccgcc accatacaaa
     1681 atacccaatc cagtctgtgt ggcgagttgt tcaccaggca ccccggcttc gcctttggtg
     1741 ccatttccgg caatggtctg aataatggtg gtctgagcat ctattc
//
LOCUS       DAOQDO010001513         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69699_length_1785_cov_1.846821, whole genome shotgun sequence.
ACCESSION   DAOQDO010001513 DAOQDO010000000
VERSION     DAOQDO010001513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69699_length_1785_cov_1.846821"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1185)
                     /locus_tag="PKZ53_20315"
     CDS             complement(103..1185)
                     /locus_tag="PKZ53_20315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42838.1"
                     /translation="MPIELTRTRVEIVFIPLSVILITLFHFASMQAASVLLHEISQRL
                     YYLPIIFAAYRFGLKGSVLCSVFAAGLYLSHISGHRHEPEKIVVNQLVEAVMFQVVGI
                     ATGLLSNAERRQVAKLEQASLDLSRAYRELRETVNLLLHTARLNSLGEMAASVAHEIR
                     NPLAAIKGAFEIVAEAVPADNPRREFVTVVETELERLNTLVSDFLKFSRPRTPEKKPV
                     SINTLISTVLIFASPQAQKRSVVFVTDLVEPLPHLLVDDEQMKQVLLNLVLNAIQAQP
                     QGGTVEVKTRTLPEIIEISIRDYGSGVPPQARDSIFEPFVTTKPDGTGLGLAIAYQLV
                     KQHGGDLKLVNVSGPGSEFVVSLPIQ"
     gene            complement(1175..1690)
                     /locus_tag="PKZ53_20320"
     CDS             complement(1175..1690)
                     /locus_tag="PKZ53_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42839.1"
                     /translation="MLKNYKEVWIGVALGGGIWALDAVMNMGIFGGYCCSHCAENAVL
                     GTMPQCLLRCLVVITTTTTGFVLWLSNRRRQQMQSLQATLDTFHQEVASPLLLIYGYT
                     RMLPAKEGFPVTRETVELIRQIQHQADRMNQLIEKLPPPGSSLTPDSKAASISTSPTR
                     TSDESLAPHAN"
BASE COUNT          431 a          457 c          459 g          438 t
ORIGIN      
        1 gggttccggg ttccgggttc cgggttttcg aatttatgtc cttttcgtct ttttcgtctt
       61 ttatgtcctt ttctgctttg ccggaaccct gcctcctgac ccctactgta tcggcaggct
      121 cacgacaaat tcactgccag ggccgctgac gttgaccagc ttcagatctc cgccgtgctg
      181 tttgacaagc tgataggcaa ttgcgagtcc gaggccagtg ccgtcaggtt tggttgtcac
      241 aaagggttca aagatcgagt cacgggcctg gggtggcaca cccgaaccgt aatcacgaat
      301 cgaaatttca attatttcgg gcaaggtacg ggtttttact tcgacggtgc cgccctgcgg
      361 ttgggcctgg atggcattga gcaccagatt gagcagtacc tgtttcattt gttcatcatc
      421 aaccagaagg tgaggcagtg gttcaaccag atcagtgaca aacaccacgc ttcgcttctg
      481 agcctggggt gaagcaaata tcaagactgt cgaaatcaga gtattgatcg aaaccggttt
      541 tttctcagga gttctgggcc gggaaaattt tagaaaatct gacaccagcg tgttgaggcg
      601 ttcaagttcg gtttcaacga ccgtgacaaa ctcacggcgc ggattatcag ccggcacggc
      661 ttcggccaca atttcaaagg cgcctttgat cgccgccagc gggttgcgaa tttcgtgggc
      721 cacggaagcc gccatttctc cgagtgaatt gagccgggcg gtatggagca agagattcac
      781 cgtttcacgc aattcccgat aggcacgact gaggtcgagc gaagcctgtt caagtttggc
      841 tacctggcgc cgttcggcat tgctcaggag tccagtggcg atgccgacca cttgaaacat
      901 caccgcctca accagttgat tgacgacaat tttttctggt tcgtggcgat gccctgaaat
      961 atgagacaaa tataaaccag cggcaaagac cgaacacaac acacttcctt taagtccaaa
     1021 ccgataggcc gcaaagataa tcggcagata atacagccgt tgtgaaatct catggagcag
     1081 cacactcgcg gcctgcatac ttgcaaagtg aaaaagcgtg atcaggatca cgcttaacgg
     1141 aatgaacacg atttcaacac gggtgcgggt cagttcaatt ggcatggggc gcaagcgact
     1201 cgtctgaagt tcgggttggg gaagttgaaa tactggcggc tttactgtca ggtgtcaaag
     1261 acgaaccagg tggcggcaat ttttcaatca attgattcat ccggtcagcc tgatgctgaa
     1321 tttgtcgaat gagttcaacc gtttcacgcg tcaccggaaa gccttctttg gctggcagca
     1381 tccgggtata gccgtaaatg agcagcaacg gcgacgccac ttcttgatgg aatgtgtcaa
     1441 gcgtcgcctg taaactctgc atctgctgcc gccgccggtt actcaaccac aaaacaaatc
     1501 cagtcgtggt ggtcgtgatc acgaccaggc agcgcaacaa acactgaggc atggtgccca
     1561 gcacagcatt ttcagcgcag tgcgagcaac aatatccacc aaaaataccc atattcatca
     1621 cggcatccag cgcccagatt ccgccgccca atgccacccc aatccagact tctttatagt
     1681 ttttcaacat aagccacccc agggagaatg aagaattgaa aatgaagaaa ctcttggttc
     1741 ttggttcttg gttcttggtt cttggttctt ggttcttggt tcttg
//
LOCUS       DAOQDO010001514         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69778_length_1783_cov_3.497685, whole genome shotgun sequence.
ACCESSION   DAOQDO010001514 DAOQDO010000000
VERSION     DAOQDO010001514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69778_length_1783_cov_3.497685"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1566
                     /locus_tag="PKZ53_20325"
     CDS             190..1566
                     /locus_tag="PKZ53_20325"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNJ42840.1"
                     /translation="MFCRCGVVVLMYMILGSFAILAQSEKPILIVRKSTKPNAPPPKP
                     RVGKTNLAISTTPPEAEVYVNGDKSGVSDSTGSLILEFTPNRTYQIEVRKPDFEPFAK
                     SYRPQVNKTEVLPVRLVAKFAPLVLAGVPEGASLMLDNQPVKLEDLRVDGKQVTFPKL
                     ATGTHTVRIEHADWMPWEDKVEIKPDDPNGNVVTPPLVAALVKVTIASVVGATVYVDD
                     ADRGTITPDGSLVIPNVRPGVRTIRVVKDDYVETKLTETLAVGEKSLKIDLTPIPSSA
                     EFSDRFNAGLGKWEAPAGWTVNKGLVVKGEGTGQPADKVYRDFDVDFDIQFLNGKGAA
                     WVVRARNTNAYVFYLSGSEGKYPGEFRGYVSRNGQLDLAKPIEAPPILTDIKANVYYH
                     VHIEVRGNRIKHELIPSSGPDAGQSVPLGFLEDPQNSFKLGNFGFRTIQGEEFQVNAF
                     FIKPVDLK"
     gene            1593..>1783
                     /locus_tag="PKZ53_20330"
     CDS             1593..>1783
                     /locus_tag="PKZ53_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42841.1"
                     /translation="MSKLRSVFASPHVWPIVIICLVTLMAYWNSLSGEFVYDDNVVIV
                     DRVEIQSLHSIPTLFKQSY"
BASE COUNT          441 a          425 c          446 g          471 t
ORIGIN      
        1 ataaacatga gtatctcggg tttcgagttg ttctggatca accggttccg tgatcattgg
       61 cccgctaaag attcgataga ctgtgtttct tttgtataaa tgaaaacctc acatctccag
      121 gcatcctatg gtgactaaga ttactcaaga acaaataaaa tcaattactt gtgaaggatc
      181 tacataatta tgttttgtcg atgtggggtt gttgtattga tgtatatgat tttgggaagt
      241 ttcgcgattt tggctcaaag cgaaaaaccg atattgattg tccgtaaatc aaccaaaccc
      301 aatgccccac ctcccaaacc tcgggttgga aagacaaatt tggccatttc aaccacccca
      361 cccgaagccg aagtctacgt caacggcgac aaatccgggg tttccgattc gaccggctct
      421 cttatccttg aatttactcc caaccgtacc tatcagatcg aagttcgcaa acctgatttt
      481 gagccgtttg caaaatctta tcgcccacaa gtaaacaaaa ctgaggtttt gccagtccgg
      541 ctggtggcca aatttgcccc gctggtcctg gccggtgtcc ctgaaggcgc cagtctgatg
      601 cttgataacc agccggtcaa acttgaagac ctgcgggttg atggcaaaca ggtcacgttc
      661 ccaaaacttg cgacggggac acacaccgtg cggatcgagc atgccgactg gatgccctgg
      721 gaagacaaag ttgaaatcaa accggatgac ccaaatggca atgttgtgac ccctccgctg
      781 gtggcagcac tggtcaaagt tacgattgca tctgtcgttg gggcgaccgt gtatgtggat
      841 gacgctgatc gggggacgat cacccccgat gggtcgctgg tcattccaaa tgttcgaccg
      901 ggtgtgcgta cgattcgtgt ggtcaaagat gactacgtcg agaccaaatt gaccgaaacc
      961 ctcgccgttg gggaaaagtc actcaagatt gacctgacgc cgattccgtc ttcggctgaa
     1021 ttcagtgacc ggtttaatgc cgggttgggg aagtgggaag cccctgccgg ttggacggtc
     1081 aacaaagggc tcgttgtcaa aggcgaaggt accggacagc cagcagacaa ggtgtatcgt
     1141 gactttgatg tggatttcga tatccagttc ttaaatggga agggggcggc gtgggtagtt
     1201 cgggcgcgca acaccaatgc ctatgtgttt tacctttcgg gctcagaagg gaagtatcca
     1261 ggtgagtttc gagggtatgt gtcccgtaat ggacagttgg atctggcaaa accaattgaa
     1321 gccccaccca ttttgaccga tatcaaagcc aatgtgtatt accacgtaca cattgaagtt
     1381 cgcggcaacc ggatcaaaca cgaactgatc cccagttccg gcccagatgc cgggcaatcc
     1441 gtaccgcttg gttttttaga agatcctcaa aacagcttca agcttgggaa ttttggtttc
     1501 cgcactatcc agggcgaaga atttcaagtg aatgcgtttt ttatcaagcc agttgatttg
     1561 aagtagttgc ctccctgtac cgccaccatt ttatgtcaaa actccgttcg gtttttgcct
     1621 cacctcatgt ctggccaatt gtcatcatct gtctggtcac gttgatggca tattggaaca
     1681 gtctctctgg cgagtttgtc tatgatgaca atgtcgtcat tgttgatcgg gtggagattc
     1741 agtcacttca ctctattccg actttattta agcaaagtta ttg
//
LOCUS       DAOQDO010001515         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69817_length_1783_cov_2.251157, whole genome shotgun sequence.
ACCESSION   DAOQDO010001515 DAOQDO010000000
VERSION     DAOQDO010001515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69817_length_1783_cov_2.251157"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKZ53_20335"
     CDS             <1..208
                     /locus_tag="PKZ53_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42842.1"
                     /translation="RLQLAAFQDDEWVALTPEGYYTASPKGDQYLNVRVGNQVLGIDS
                     FRNKFNQPERIKQVFSQFPAQQGK"
     gene            223..594
                     /locus_tag="PKZ53_20340"
     CDS             223..594
                     /locus_tag="PKZ53_20340"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNJ42843.1"
                     /translation="MSNDQAELMEVFVYGTLMRGESNHHHLAGCEFLGEDILEQAQLW
                     SAGPYPALRQGEGSVIGERFRVTPEVLVQLDWLEDHPRLYLRREVTLKSGATVWVYFG
                     NRIFRHQLVRLPENRWKSPAV"
     gene            641..1075
                     /locus_tag="PKZ53_20345"
     CDS             641..1075
                     /locus_tag="PKZ53_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42844.1"
                     /translation="MIQPGLSFEFTLPERWSSDHDRGWLIVHGFEDEELIISGKTVEG
                     FDPTEDPGKIVQEVLNGAINAMLHAVDIPELCVTQPLEQASQLTDLDCWTLRAQTQDA
                     AILFFQSVFCSDNGILLVTFEAPNTPASALQYQKFLQTVRKT"
     gene            1262..>1783
                     /locus_tag="PKZ53_20350"
     CDS             1262..>1783
                     /locus_tag="PKZ53_20350"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNJ42845.1"
                     /translation="MDQSLTAITGSALRKVGTGWEFASEAALEQFVWHHLNEVLGLRP
                     VARQFFCLEEICDIVGIDELQRLTIVELKNTEDRYVVQQLHRYFFNLREQKAFADQVD
                     WSKPPRLIAVAPSFHRHNRIDQEFSQLEIEFVRFAVTKETDQFFLNLFEGRATEPRKV
                     RIPYLELDLPATPI"
BASE COUNT          469 a          440 c          436 g          438 t
ORIGIN      
        1 acggctgcaa ctggcggcct tccaggatga tgaatgggtg gcactcacac ctgaaggtta
       61 ttacactgct tcacccaagg gagaccagta tctcaatgtt cgggttggca atcaggtcct
      121 gggaattgac tctttccgca acaaattcaa tcagccggag aggataaagc aggtattttc
      181 tcaatttcct gcccaacaag ggaaataagc agccagctat atatgtcaaa tgatcaggct
      241 gaactgatgg aagtctttgt ctacggcacg ctgatgcggg gtgaatcaaa ccaccatcac
      301 ctggctggat gtgaatttct gggtgaagac atcctcgaac aggcccaact ctggtccgcc
      361 gggccgtatc cggcgctccg tcagggcgaa ggatcggtca tcggtgaacg ctttcgggtc
      421 acaccggaag ttctggttca acttgactgg cttgaagacc atccccggct gtatcttcgc
      481 cgtgaagtca cactcaaaag cggcgctacg gtatgggtct atttcggcaa ccggattttt
      541 cggcaccaac tggtgcggct gcctgaaaac cgctggaagt caccagcagt gtaacgggtg
      601 ggatttcgtc aacttttcag cctgaacagc aactccgatc atgattcaac ctgggctcag
      661 ctttgaattt acccttccag aaaggtggtc ctcagaccat gatcggggct ggctgatcgt
      721 tcacgggttt gaagatgaag agttgatcat ttcaggaaaa accgttgaag gctttgaccc
      781 aacagaagat cctggaaaaa tagttcaaga agtactgaat ggagccatca atgccatgct
      841 ccatgcggtt gatatcccag aattatgtgt aacccagcca cttgaacaag cgtcacagct
      901 taccgatttg gattgctgga ccttacgtgc ccaaacccag gacgcagcga tattgttttt
      961 ccaatcagtg ttttgcagtg ataatggcat tttactggtt accttcgaag ctccaaatac
     1021 accagcgtca gcgctgcagt atcaaaaatt tcttcaaaca gtgcggaaaa cttagtcaca
     1081 cttttttcat tgcttcgtgg atcacatctg tttttcccaa tcagtgctgt caggtgtggt
     1141 gtcaacggcg aagcagtggc aaaatgctaa cctgtagcgt cagtcacagg attgaagaaa
     1201 taaatcaaca agccgcgaaa cggtgaaaga atattggtta caaaatggaa ggcatcaggt
     1261 aatggatcaa tcacttacgg caataaccgg gtcagcctta cgcaaagtcg gcaccggctg
     1321 ggaatttgct tcagaagccg cgctcgaaca gtttgtctgg catcacctga acgaagtttt
     1381 agggcttcgg ccagttgccc gtcagttttt ctgccttgaa gaaatctgcg atattgtggg
     1441 cattgatgaa ttacagcgac tgactatcgt agagctaaaa aacaccgaag atcgctacgt
     1501 cgtccagcaa ctccaccggt atttcttcaa cttgcgcgaa caaaaagcct ttgcggatca
     1561 ggtagactgg tcgaaaccgc cgcgtttgat tgcagtcgca ccgtccttcc atcgccataa
     1621 ccggattgac caggaatttt cacaacttga gattgaattc gttcggtttg cggtaacgaa
     1681 agaaaccgat caatttttcc tcaatctgtt tgaaggacgg gcaaccgaac ctcgcaaagt
     1741 acggattccc tacctggaac tcgacctccc ggcaacgccg att
//
LOCUS       DAOQDO010001516         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69854_length_1782_cov_3.172554, whole genome shotgun sequence.
ACCESSION   DAOQDO010001516 DAOQDO010000000
VERSION     DAOQDO010001516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69854_length_1782_cov_3.172554"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKZ53_20355"
     CDS             complement(<1..543)
                     /locus_tag="PKZ53_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42846.1"
                     /translation="MPILLFIFALSSPVWAQSGGRSKQNPPFDPQTKPQAKPGGTNLV
                     PQQSRTFFVVTSPHDDSKPYPLEDTVRNACQDELRSISGVKIIENENVLRWEAKDLAF
                     SADNQTWVIWIELKFNDLDLRHPDFLKVQYLLFESGSGRVLSGGFASTSAKNWQSQNP
                     WPWPASYVDLRVPANYAGRDV"
     gene            1000..>1782
                     /gene="proS"
                     /locus_tag="PKZ53_20360"
     CDS             1000..>1782
                     /gene="proS"
                     /locus_tag="PKZ53_20360"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNJ42847.1"
                     /translation="MHWHNLFIPTLREAPADAEAVSHKLLVRAGFIRQLTAGAYSILP
                     LAQRTILKITDIVRHEMNAIGGQEFYLPALNPASIWRESGRWDVMGDNMFRLKDRKGA
                     ELCLGMTHEEVFTSIARHEVRSYKELPQIWYQIQTKFRDEPRPKSGLLRVRQFTMKDS
                     YSFDIDQAGLDQSFDLHDAAYRRIYSRCGLKFAVVEASSGAMGGSASSEFMVASDAGE
                     DWIASCPECGYAANVEKATSNLPVIEDGEGLPAPEKFPTPNVR"
BASE COUNT          454 a          460 c          445 g          423 t
ORIGIN      
        1 aacatcacgg cctgcatagt tagctggcac tctcaaatcc acataacttg ctggccaggg
       61 ccacggattt tgtgattgcc agttcttggc ggatgtactg gcaaatccac cgctcagaac
      121 ccttccagag cctgattcaa acagcaaata ctgaactttg agaaaatccg ggtggcgtaa
      181 atccaggtcg ttgaatttga gttcaatcca gataacccac gtctgattat ctgcagagaa
      241 agccaggtct tttgcttccc aacgcaaaac attttcattc tcaatgattt tcactcctga
      301 aatggatctc aattcatctt gacaagcatt gcgcacagta tcttcaagcg gatagggctt
      361 tgaatcatca tgaggtgacg tcacaacgaa aaaggtccgt gactgctgag gaaccaaatt
      421 ggtgcctccg ggttttgcct ggggttttgt ctggggatca aacggtggat tttgttttga
      481 tcggcctccg gattgcgccc agacaggtga actcagagca aaaatgaaca gcagaatggg
      541 catccatcgt gtccaggaca gccaatcatg cgaatgagaa tgactggaaa tctcagcaca
      601 ggacatagca tggcctcctt tgagaggaag ttttgaaggc taggaacggg aaaagtcagg
      661 attgattgag gttattgaat tgttttgggg atgaggaatc cgccagcgcc tgtgatgcac
      721 aatccccgtc aatgggtgga ttctctttaa aaataaagag ttcacgtcac tgcaagacac
      781 atctccgttg acagaaagac ggcttccgcc ataccctaca cgctttttca gatacatacc
      841 ggtatccagc atgctgcaaa cccggaaccc ggaatcctga caagatgacc gactgacaag
      901 gtgaccaggt gattttttca tccctcatcc ttcatccctc atcccttcag aaacccggag
      961 cccggaatct cacttcacct caggacacat caagacacta tgcactggca caatctgttt
     1021 attccaactt tgcgtgaagc tccagccgac gctgaggctg tgagccacaa gcttctggtg
     1081 cgcgccggtt ttatccggca actgacggca ggcgcttatt caatcctgcc gcttgcgcag
     1141 cgcacaattt tgaaaatcac cgacatcgtt cgtcacgaaa tgaatgccat cggcggccag
     1201 gaattttatc tcccggcact taatccggct tcaatctggc gcgaatcggg ccggtgggat
     1261 gtgatgggcg ataatatgtt tcgcctcaaa gaccgcaaag gcgcggaact ctgccttggg
     1321 atgacccacg aagaggtctt tacctcgatt gcccgccacg aagtgcgatc ctacaaagag
     1381 cttccgcaaa tctggtatca gattcagacc aagttccgtg acgaaccccg tccaaagtct
     1441 ggattgctgc gggtgcggca gttcacgatg aaagattcat attcatttga cattgaccag
     1501 gctgggctag atcaatcctt tgacctgcac gatgccgcct atcgtcggat ttacagccga
     1561 tgcggcctca agtttgccgt tgtcgaagcc agcagcggtg cgatgggtgg gagcgcatca
     1621 agcgaattta tggtggcttc agacgcgggt gaagactgga ttgccagttg cccggaatgt
     1681 ggctatgccg ccaacgttga aaaagcaacc tcaaacctgc ctgtgattga ggacggcgaa
     1741 ggtttgccgg cacctgagaa attcccaacg cccaatgtgc gc
//
LOCUS       DAOQDO010001517         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69927_length_1781_cov_3.914253, whole genome shotgun sequence.
ACCESSION   DAOQDO010001517 DAOQDO010000000
VERSION     DAOQDO010001517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69927_length_1781_cov_3.914253"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKZ53_20365"
     CDS             complement(<1..777)
                     /locus_tag="PKZ53_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42848.1"
                     /translation="MVIRRSLSLILAVLAVYSVNIFHQPLTFVPLAGGVEANGKSQTA
                     ADFQPPVFSPANEGMPSLSGEKAVTYLKEQGGYDSLGDAIAAARYSANWANTTRIPTV
                     SGGAYEADNPAQGFTSYFTPEGVHLVAAGEGTPAWFLEMKLQGIGYGDQLGDVSASVP
                     AVTSNRVELVRNSALSPEPLALALNSSLIPHPSSLALTSAPSPEPSALIEWYENGPAG
                     LEQGLTLDAPPGERSGADRLRVVFDVDGTLEARRTADGQVI"
     gene            complement(926..1519)
                     /locus_tag="PKZ53_20370"
     CDS             complement(926..1519)
                     /locus_tag="PKZ53_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42849.1"
                     /translation="MKSLIWLLVLNAILLTGITTNQTQAEAEFPFAFQLQVVTFSDGS
                     KQFSLNPDPLEGDIRARGDYLRWEDTTFHGKQALVITFHIVSWRKDQVMELKERMGIN
                     LNAFPLVEGATQKRDRHRGYPGAYKIQIITFKQKNPQIVITPNPIERMDFNDKALVKC
                     EENQIDSATGEKCRVCTLYFLEWNPVTVQQVVKLMDP"
BASE COUNT          424 a          461 c          444 g          452 t
ORIGIN      
        1 gatcacctgt ccatcggctg ttcgccgggc ttccagtgtt ccatccacat cgaagaccac
       61 ccgcaaccgg tcggccccgc ttcgttcccc aggtggtgca tcgagcgtca gtccctgttc
      121 caacccggct ggaccgtttt cataccactc aatcagggct gagggctcag ggctcggggc
      181 tgaagtcaga gccagggatg agggatgagg gatgagggat gaattcaaag ccagggctaa
      241 gggctcaggg ctaagggctg aattccgaac cagttctact cgattgctgg tcacggctgg
      301 tacgctggct gagacgtctc ccaactggtc gccatagccg attccctgga gcttcatctc
      361 cagaaaccac gctggtgtgc cttccccggc agccaccaga tgcacccctt ctggcgtgaa
      421 gtaactggta aacccctggg ctggattgtc ggcttcatac gccccgccgc tcacggtggg
      481 gattcgcgtc gtattggccc agtttgcact gtacctggcc gcggctatgg catcacccaa
      541 cgaatcgtac cctccctgct ccttcaggta cgtgactgct ttttccccac tcaacgacgg
      601 cattccctcg tttgctgggg aaaacactgg gggctggaaa tcagccgccg tctgagattt
      661 gccgttcgcc tcaactcccc cggccagcgg tacaaaggtc agaggttgat gaaaaatatt
      721 gacactgtac acagccagca ccgccagaat aagcgataga gatcgacgaa taaccatagt
      781 tcattccttg tttttcggtt tggaatactg ctttttggag attggattgg tcgaggcaaa
      841 aaccactgac agggcaagcg aataagaaag aacaaacgaa ttctctttct tctgttgtgc
      901 ttcaaataga gtgaacttga atgggttatg ggtccatcag tttaacaact tgctgtacag
      961 tgaccggatt ccattccaga aaatacaggg tgcacaccct gcacttttcc ccagttgcgg
     1021 aatcaatctg attttcttcg cacttcacca gtgctttatc attgaaatcc attctttcaa
     1081 ttgggttagg ggtaataaca atctgcgggt ttttctgttt gaacgttatg atttgaatct
     1141 tgtaggcgcc tggataccct cggtgccggt ctcgtttctg cgtagcgcct tcaaccaaag
     1201 gaaaggcgtt gagattaatc cccatccgtt ccttcaattc catgacctga tcttttctcc
     1261 aggacacaat atgaaacgta atgaccagcg cctgtttgcc gtgaaaagtc gtatcttccc
     1321 accgcagata atcacctctc gctctgatat cgccctccaa tggatcagga ttgagactaa
     1381 attgcttgct tccatctgaa aaagtgacga cctgcagttg aaaggcaaac gggaattcag
     1441 cttcagcctg ggtttgattg gtggtgatgc cagtgagcag aatcgcattc agtaccagca
     1501 accagataag tgatttcatt tcacaatccg agtccagaac attcaaagtc ttctccaatt
     1561 cgatcaacca ggaaaacaat cagtgacacg ttcactggcc ctcgtgcttt cctggaagca
     1621 atcgaaaagg attgtggaca cggagaagga aatctggaat ccagggcact actcattttt
     1681 tatgagtagt actactcatt ttttggagtt gagtggagtt ggggaacgtt tggaggggag
     1741 aaagatttcg ggatgacgtc accggtcttg aggatgacac c
//
LOCUS       DAOQDO010001518         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_69956_length_1781_cov_2.533024, whole genome shotgun sequence.
ACCESSION   DAOQDO010001518 DAOQDO010000000
VERSION     DAOQDO010001518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69956_length_1781_cov_2.533024"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="PKZ53_20375"
     CDS             <1..1560
                     /locus_tag="PKZ53_20375"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ42850.1"
                     /translation="THNSKRCDMNRDSWRLSTDVKILLFLAGLKLLIHLFAIKGYGYF
                     RDEFYYLACGEHLAFGYVDHPPMVAVVAKLATTLFGESLLAIRILPALCGATITFLTG
                     YLTGELGGNRAAQILAAIASIGAPVFLNIHNYLSMNAFDHLFWLLGALVLVAILKQDE
                     PKLWVWFGLVAGLGLENKVSMLFLCFGVFVGLLLTPARKYFLSKWIWIGAGLALLLFL
                     PNIIWQITHDFPTLEFMRNAQRYKNYQISPSEFLIQVTLEANPFTLPLTLGSLFFFFV
                     LREGKPFRLLGWMFVAILGLFLVQKAKPYYLAPILPVLFAAGGVSIEILSRQRWGRFL
                     PPTYATVLVVGGIIVAPLAMPLLPEETFISYAKSLKITGTKSENHKMGKLPQFFADMH
                     GWEKMVVTVAEVFHKLPAEEQKKCGIYAQNYGEAGAVDFFGKRYGLPKAICGHNNYFL
                     WGPGTQTGEVMIIIGGDAEDHEQSFEEVKIEGVFVDEYAMPYENNLPIFVCRRLKKPM
                     TEVWPKTKHFI"
BASE COUNT          413 a          398 c          471 g          499 t
ORIGIN      
        1 actcataatt ccaagaggtg cgatatgaac cgggattcgt ggcgtctttc gactgatgtg
       61 aagattttat tatttctggc cgggcttaaa ctcctgattc atttatttgc catcaaaggc
      121 tacggctatt ttcgggatga gttttactac ctggcatgtg gcgagcatct cgcctttggg
      181 tatgttgatc atccgccgat ggtggccgtt gtggctaaac tggcaacgac attgtttggt
      241 gagtcgctgc tggcaattcg gattttgccg gcattgtgcg gtgcgacaat cacctttttg
      301 accggctatc tgacaggtga attaggtgga aatcgagccg cgcaaattct ggcagccatc
      361 gcttcgattg gggcgccggt gtttttaaac atccataact acctgtcaat gaacgccttc
      421 gatcacctgt tctggctcct tggagcgctg gtgcttgtag cgattctcaa acaggatgag
      481 ccaaaattgt gggtctggtt tggtctggtc gcaggactgg ggctggaaaa taaagtttct
      541 atgctctttc tgtgtttcgg tgtgtttgtc ggactgctgc tcacaccggc cagaaaatat
      601 tttctttcca aatggatttg gattggtgct ggactcgcct tgcttttatt tcttccaaat
      661 atcatctggc aaattacgca tgactttcca acgcttgaat ttatgcgcaa tgcccagcgc
      721 tataaaaact atcaaatctc cccatcggag tttttgattc aggtcacgct ggaagccaat
      781 ccgttcacgc ttccgttgac gctggggagt ttgttctttt tctttgtttt gcgtgaagga
      841 aaaccatttc ggctcctggg ctggatgttt gtggcaattc tgggattgtt tttagtccag
      901 aaggcaaaac cctactatct ggcaccgatt ctgccggtgc tgtttgcggc tgggggcgtt
      961 tcaattgaaa tcctgagtcg tcaacggtgg ggacggtttc tccctccgac atatgctacg
     1021 gtactggtgg tgggtggaat catcgttgca ccgctcgcca tgccgctact gccggaagaa
     1081 accttcatca gctatgccaa atcactcaaa atcactggca ccaagtctga aaatcacaaa
     1141 atgggcaaat tgccacaatt ttttgctgat atgcatggct gggaaaaaat ggtggtcacc
     1201 gtggcagagg tatttcacaa actgcccgcc gaagagcaga aaaagtgcgg aatttatgcc
     1261 caaaactatg gtgaagccgg ggccgtggac ttttttggca aacgctatgg attaccgaaa
     1321 gccatctgtg ggcacaacaa ttatttcctt tggggacccg gcactcaaac cggcgaggtc
     1381 atgatcatta ttggtggaga cgccgaagat catgaacaat cttttgaaga agttaaaatt
     1441 gaaggcgtgt ttgttgatga gtatgcgatg ccctatgaaa acaacctgcc gatctttgtt
     1501 tgccggcgct taaagaagcc gatgactgag gtctggccaa agacaaagca ctttatttag
     1561 tggggttccg ggttccgggt tccgggttcc gggttcttcg aagggatgag ggatgaggga
     1621 tgagggatga aagaaatcac cttttcaccc tatcagtgta tcctggtttt cgccgcgtag
     1681 cggtggctga attgaggcag gtcgtttacg acctgtaaat agagatcaaa attcgaaaac
     1741 ccggaacccg gaacccggaa cccggaaccc ggaacccgga a
//
LOCUS       DAOQDO010001519         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70017_length_1780_cov_2.844058, whole genome shotgun sequence.
ACCESSION   DAOQDO010001519 DAOQDO010000000
VERSION     DAOQDO010001519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70017_length_1780_cov_2.844058"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKZ53_20380"
     CDS             complement(<1..543)
                     /locus_tag="PKZ53_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNJ42851.1"
                     /translation="MPHSTPASEPSFDTARDTAGLGGHPKGLTMLFFTELWERFSYYG
                     MRAILMLYMTSAVAQGGLGFDVKTAASIYGTYTMSVYLTSLPGGLIADHLLGARMAVL
                     LGGIIIACGHFAMIFSSMPFFYGGLVLIAVGSGLLKPNISSMVGGLYGPNDPRRDSGF
                     SIFYMGINIGAVLAPLVCGYL"
     gene            complement(602..>1780)
                     /locus_tag="PKZ53_20385"
     CDS             complement(602..>1780)
                     /locus_tag="PKZ53_20385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNJ42852.1"
                     /translation="LTDIYEPVIAYINECRSRKTHRVPVCSLDLPSGLAADQGNPIGP
                     VVEADLTVTFTAPKLANVLPPACDYNGELRVVAIGSPKSLTRSAGKHKSWLHFVEAHD
                     VKRMLGVTRRRPGAHKGSVGHVLLAAGARGKTGAAALAGEAALRAGTGLVTVALPRSA
                     QHMLVASCLPEVMTFGAAETERGIFSESAAVQVLELAAERTVLAIGPGISSEEEDVRL
                     FVRHMVEKSQKPVVIDADGLNCLSPWPAELKGTPERPLILTPHPGEMARLTGLSTKTL
                     IRNRHDCVRALATEHHLHVVLKGQRTLIAAPNGEVYINPTGNAGMATGGSGDVLTGLL
                     AGLLAQSPGSPLESTLIAVYLHGLAGDKAAAKLGMRSMVASDITAALSDAFWEMEPSS
                     "
BASE COUNT          466 a          513 c          443 g          358 t
ORIGIN      
        1 taaatatcca cagaccagtg gtgccaggac agcgccaata ttgattccca tataaaaaat
       61 agagaatcca ctgtcccggc gtgggtcatt tggaccatac aacccgccga ccatggaact
      121 gatatttggc ttgagcaacc cagaaccaac agcaatcaaa accagtccgc catagaaaaa
      181 aggcatggac gaaaaaatca tcgcaaaatg cccgcaggca atgatgatgc cgccaagcaa
      241 gacggccatg cgggcaccga gcaggtgatc agcaatgagc ccacctggta agctcgtcag
      301 gtagaccgac atggtgtagg tgccataaat tgaggcagcc gtcttcacat caaacccaag
      361 ccctccctgg gcaactgcgg atgtcatata gagcatgagg atggcgcgca tcccgtagta
      421 gctaaaccgt tcccagagtt cagtaaaaaa aagcatggtc aatccttttg gatgaccacc
      481 aagacccgcg gtatcacggg ccgtatcaaa ggaaggctca ctggcaggcg tcgagtgggg
      541 cattgaaaac gtacgctctc ctcacaaata aattttgatc gctgataaaa acccggcctg
      601 gctatgacga aggttccatt tcccaaaaag cgtcgctcaa ggcagccgta atgtctgaag
      661 caaccattga gcgcattccc aatttggccg ccgctttatc tccggccagg ccgtgcaaat
      721 agacggcaat caaggtggat tcgagtggcg aacctggcga ctgagccagc aaaccagcca
      781 gcaaaccagt taacacatcg ccagacccac ctgtcgccat tccagcattg ccggttggat
      841 tgatatagac ctctccattg ggtgccgcaa tcagggtgcg ctgccccttg agcacaacgt
      901 gcagatggtg ctcagtggcc agggcacgaa cgcaatcgtg tcggttccga atcaaggttt
      961 tggttgaaag ccctgtcaaa cgcgccattt caccaggatg aggtgtcaaa atcaaaggtc
     1021 gttcgggtgt acctttgagc tccgccggcc agggagataa acaattcagc ccgtcagcat
     1081 caatcaccac tggtttctgg cttttttcaa ccatgtgacg gacaaacagt ctgacatctt
     1141 cttcttccga ggaaatacca ggcccaatcg ccagcacggt tcgttctgca gccagctcca
     1201 aaacctgaac agcggctgat tcagaaaaaa ttcctcgttc agtttcagcc gccccaaagg
     1261 tcatgacttc tggaaggcag cttgccacga gcatatgttg ggctgaccgt ggaagtgcca
     1321 ctgtcaccag tccggttcca gcccgtagcg cagcttcccc agccagcgcc gcagcgccag
     1381 ttttcccacg cgctccagcc gccagcagga catgcccgac agagcctttg tgtgccccag
     1441 gccggcggcg ggttacccca agcatgcgct tcacatcgtg ggcttccacg aaatgaagcc
     1501 aggatttatg ttttccagcc gaccgggtca gagattttgg cgatccaatc gccaccacgc
     1561 gcaactcacc attgtagtca caggccggag gaagcacatt ggccagcttt ggggccgtaa
     1621 acgtcaccgt gagatcagct tcaaccactg gcccaattgg atttccctga tcagccgcca
     1681 accccgacgg taaatccagt gagcaaactg gcacacgatg ggttttccga cttcgacatt
     1741 catttatata ggcaatgaca ggttcataaa tatcggtcag
//
LOCUS       DAOQDO010001520         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70036_length_1780_cov_2.410435, whole genome shotgun sequence.
ACCESSION   DAOQDO010001520 DAOQDO010000000
VERSION     DAOQDO010001520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70036_length_1780_cov_2.410435"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1780
                     /locus_tag="PKZ53_20390"
     CDS             <1..>1780
                     /locus_tag="PKZ53_20390"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42853.1"
                     /translation="GNLLVADTQNHRIRRIDPQTGIISTAVGTGEFGFDGDDGPADEA
                     QLALQFAGAIRIADDDDLLIADTFNNRIRRVDSDTDIITTVAGIGPTGNFNGDFTGDD
                     GPATEASLNVPIALALDEDQNLFIADLANNRVRRVDAETGIITTVAGNGENSGGDDGP
                     AVEAGLVPCGIVVDGFGNLYISDDGQNLIREVDAETGIITTIGGGGGDLGDNRPAIMA
                     NLSLQDTSGIALDQQGNIYFADDGNHRIRKIDIQTGIITTVAGNGRNGYSGDGGPATQ
                     ASLNDPSGMAVDQAGNILIADSSNSVIRRVDARTGIITTVAGNGGFDFDGDGGPALEA
                     TLNFPTDVEVDQSGNIYVVDSNNNRIRKVDARTGIILTVVGSGPAGEPADSGDGGPAT
                     SARLQYPRSLLIDSEGNLFVADRDAGRVRRVDASTRIIRTIAGGGENDDDNILATQAQ
                     LFGPHRMAMDGAGNLFISEAYASRIRRVDAQTGLVSSFSGFGIPGYSGDEGPADQAML
                     ASPQGVAIDKLGNVVILDTGNNAIRTVKEGAAPAGGGGNPDTQNPAVTDVTLLKTKGK
                     RKKDASLGNSWTSADNTGVTGHDLSFA"
BASE COUNT          416 a          463 c          495 g          406 t
ORIGIN      
        1 acggaaatct tctcgtcgcc gacacgcaaa accatcgcat ccgccgcatt gatccccaaa
       61 ctggaatcat ctccaccgcc gttggaactg gagaatttgg gtttgacggt gatgatggtc
      121 cggctgacga agcccaactg gcactccagt ttgccggtgc aattcggatt gctgacgatg
      181 atgacctgct gattgctgat acttttaaca accgcatccg gcgtgttgat tctgacaccg
      241 acatcatcac taccgtggct ggcattggtc caactggcaa ttttaatggc gactttaccg
      301 gtgatgatgg cccagccact gaagcatcgc tcaatgttcc gattgcactg gccctggatg
      361 aagaccagaa cctgtttatt gccgatcttg ccaacaatcg cgtccgacgt gtggacgctg
      421 aaaccggcat tatcaccact gtggccggaa atggcgaaaa ttccggcgga gacgatggcc
      481 cggcggttga agctggactc gttccctgcg gtatcgtggt tgatggtttt ggaaatctct
      541 acatttccga cgacggtcaa aatctgattc gagaggttga tgccgaaacc ggcattatca
      601 cgaccatcgg tggcggtggc ggtgatcttg gcgacaatag accggcaatc atggccaatc
      661 tcagcctgca ggatacttca ggcatcgccc ttgaccagca agggaatatt tactttgcgg
      721 atgatgggaa tcatcggatt cgcaaaattg atattcaaac cggcattatt acgaccgtcg
      781 ccggaaatgg ccgaaacgga tattccggcg atggcggccc agccacccag gcatcgctca
      841 atgatccatc tggaatggca gtggaccagg ccggaaatat tttgattgcg gattcgagta
      901 attccgtcat tcgccgggtt gatgcccgga ctggaatcat caccactgtc gctgggaatg
      961 gcgggtttga ctttgacggc gacggcggcc cggctcttga agcaaccttg aatttcccaa
     1021 ccgatgtgga agttgaccag tctggaaaca tctatgtcgt tgactcaaac aataaccgaa
     1081 ttcgtaaagt tgatgcccga actggaatca tcctgactgt ggttggaagt ggcccggcag
     1141 gtgagccggc agacagcggc gatggcggcc cggcaaccag tgcccggttg caatatccac
     1201 gaagcctgtt gattgatagc gaagggaacc tgtttgtggc tgaccgggat gctggtcgcg
     1261 tccgtcgggt tgacgccagt acgcgcatca tccgcaccat cgctggtggt ggtgaaaatg
     1321 atgacgataa cattttagcc actcaggctc agctttttgg cccccatcgc atggcaatgg
     1381 atggtgccgg taatctcttc atttctgaag cctatgcgtc gcggattcgc cgggttgatg
     1441 ctcaaacagg tctggtttca agctttagcg gctttggcat tccaggctac tctggcgacg
     1501 aaggcccagc cgatcaggcc atgctggctt cacctcaggg agttgccatt gataagctcg
     1561 ggaatgtggt gattctggat accggaaaca atgccattcg aacggtgaaa gaaggcgcgg
     1621 caccggctgg tggcggtgga aacccagata cccaaaaccc agcagtaacc gacgtgacgc
     1681 ttttaaaaac caaaggcaaa cgtaaaaaag acgcatcgct gggcaattcg tggacttcag
     1741 ccgataatac cggcgttaca ggtcacgacc tcagctttgc
//
LOCUS       DAOQDO010001521         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70094_length_1779_cov_3.022622, whole genome shotgun sequence.
ACCESSION   DAOQDO010001521 DAOQDO010000000
VERSION     DAOQDO010001521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70094_length_1779_cov_3.022622"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKZ53_20395"
     CDS             <1..225
                     /locus_tag="PKZ53_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42854.1"
                     /translation="DGPMKVKTFESSVNVMLDDSQELVINNQGQVQKYSLKDMNDGET
                     RTFTSGNDTILVTKKDGHFQVMVNKQGSEN"
     gene            complement(413..>1779)
                     /locus_tag="PKZ53_20400"
     CDS             complement(413..>1779)
                     /locus_tag="PKZ53_20400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ42855.1"
                     /translation="LSGQVIDFHEVPPKFADTRYADHWESAEQRELAKESAKQRPVPH
                     PIINYETCIGCHACILACPQDVLGFDEQEHHAVVVNYEQCMEDTGCQQACPTVPQSCV
                     LINTKLEIREPPRPLRKSAAEGFETLDVPGIYLVGDVSGVPLIRNAIREGRTAIDKIA
                     EVVNQQPGPPDVAYDVAIIGIGPAGLAATARASEKGLRYLALEQGRRYATIAEKYPAG
                     KYVAFNPFVPDDSQLGPIKLEGTGDLKERMISWWDESVAHLGLTIHEFEGCTGLEQNE
                     GFFVVKTVKNPDGYRARKVILAIGNAGEPRKLGVPGETAARVRYRLKAASDFQNQKVM
                     IVGAGNSAVEAAVDLAGKRQPDGTVVFPEEGGNEVTLVIRSDFPKDLTLENKMWVYYC
                     IDKGRIKAFFGAAIREIRDDDVVLESARGKKELAVVPNDVVFAMVGSVPPKEFLSKVG
                     IKYV"
BASE COUNT          437 a          493 c          406 g          443 t
ORIGIN      
        1 gatggcccaa tgaaagttaa aacgtttgaa tcatcggtca acgtgatgct tgatgattct
       61 caggaactgg tgatcaataa tcagggccag gttcagaaat actcactgaa agatatgaat
      121 gatggtgaaa cccgcacctt tacctctggc aacgacacca ttttggtgac caaaaaagat
      181 ggtcacttcc aggtaatggt caacaaacag ggttccgaaa actaatctca ggatttttga
      241 ctgcctctcg aaagatggcg tggggtttga ggtttgagaa cacattctta acctcaagcc
      301 cccatttttt tgtctcaatt ctcccggttt atagtcagca ccccgtcctt gacgtagcgg
      361 aaaacataac ccgcccacgt ccggaaacct ggaacccgga accctgaatt cctcacacat
      421 acttaatgcc aactttggac aggaattctt tcggcgggac acttccgacc atagcaaaca
      481 caacatcgtt tgggaccacc gccagttctt ttttgccacg ggctgattcc agcaccacgt
      541 catcatcccg aatctcacgg attgccgccc caaaaaaagc cttaattcgg cctttatcaa
      601 tgcagtaata gacccacatt ttgttttcca gagtcaggtc ctttggaaaa tcagaccgaa
      661 tcaccagtgt gacctcattc ccaccttctt ctggaaaaac aacggtgccg tcaggctggc
      721 gttttccggc caaatcaacg gcagcttcaa cggctgaatt cccagcgccg acaatcatca
      781 ccttttgatt ttgaaagtca ctggcggctt tcaggcgata acggacccgc gcagcggttt
      841 cgcctggaac accaagtttt cgtggctccc cggcatttcc aattgccaga attactttgc
      901 gggcgcggta tccgtctgga ttcttcacgg ttttgaccac aaagaatcct tcattttgct
      961 caagcccggt gcagccctca aattcgtgaa tcgtcagccc aagatgggcc actgactcat
     1021 cccaccagga aatcatgcgt tctttgaggt caccggttcc ttctaattta attgggccca
     1081 attgtgaatc atcaggaaca aacgggttaa aggcgacata ttttccagcc ggatatttct
     1141 cggcaatcgt ggcatagcgg cgtccctgtt ctaatgccag atagcgcaag cctttctccg
     1201 aggctcgggc tgtcgccgcc agacctgccg ggccaatccc aataattgcg acatcgtatg
     1261 cgacatcagg cggacccggc tgctggttga ccacttcggc aattttgtca atcgcggtgc
     1321 ggccttcccg aatggcattt cggatcaatg gtaccccaga gacatccccg accagataaa
     1381 ttccaggtac atccagggtt tcaaatcctt cggcggcact tttccgcagt ggtctggggg
     1441 gttcacgaat ttccagcttg gtattgatca aaacacacga ttgcggcacg gttgggcagg
     1501 cttgctgaca tccggtatct tccatgcatt gttcatagtt gaccaccacg gcgtgatgtt
     1561 cctgctcgtc aaacccaagc acatcctgcg ggcaggccag aatacaggcg tggcatccaa
     1621 tacaggtttc ataattgatg attggatgag gaacaggccg ctgtttggcc gattctttcg
     1681 ccaactctcg ctgctcagcc gattcccaat gatcagcata tcgggtatca gcaaatttgg
     1741 ggggcacctc atgaaaatcg atcacctgac cactcaggc
//
LOCUS       DAOQDO010001522         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70116_length_1779_cov_2.502900, whole genome shotgun sequence.
ACCESSION   DAOQDO010001522 DAOQDO010000000
VERSION     DAOQDO010001522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70116_length_1779_cov_2.502900"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="PKZ53_20405"
     CDS             <1..1754
                     /locus_tag="PKZ53_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42856.1"
                     /translation="SAVLSTVPEGEFLEHNLNFFCTTIVEGFTRFEWAGFLDAQTATN
                     IKTLIEVYPSVALKLERFLVGLIYQTDSFNTLLTRLRAIDYFQAVIDFCRQSSAGHEL
                     DTQVAKLAGQMADHLHSAFQADLKNGVSAPSREKCTRDLAFVLNALADGLKRLPAPAA
                     LLNVHRYLTEQVSPFIAYGEDVWKMTWQAAWFHAEKIFPPATYRELKTWFRSLEKIGK
                     PMVLIGALNQKFVCSQEPIFSPNPDEETEWRQTVCTLLALAQINAASPKSLSAIRWIL
                     AESLPLTKSQDFPEKLALILTSLRNWFIDLDLSPIQAQFEAFEQVLADLGTLRKFSLA
                     VNDVRQKLTERLPAVQELGSRERVNSFLFWMGRNALYGSAEPASRWGVPPEFKFLSES
                     GFLKLGTLTTSEIERLQAALLNSFAVHLPRISELAPTIQRLQQLAQTEKDLRQIWSRQ
                     WQVEGKEFSHASCQRDLQWMARRVCIDGWLGHRTAEGAIAWYLNEVIPFAGQITPKNY
                     IKAMKLLFEKFQTSNLVLPDMKTAADNFQTSVSTTLSSPKKVDPTVLKEFQPPEDEPQ
                     SLWKKISGAFSRPRLDA"
BASE COUNT          424 a          470 c          460 g          425 t
ORIGIN      
        1 cctcagccgt gctcagcacc gttccagaag gtgaatttct ggaacacaat ctcaattttt
       61 tctgcaccac gattgtcgag ggctttacgc gctttgagtg ggctggattt cttgatgctc
      121 agacagccac caacatcaag actttgattg aggtgtatcc gtcagtggca ctcaagcttg
      181 aacggtttct ggtcggcctg atttaccaga ctgattcctt caataccctg ctcacccggc
      241 tgcgggcaat tgactatttc caggcagtca ttgatttctg ccgtcagagc agcgccggac
      301 acgaactgga cactcaagtg gccaaactgg ccgggcagat ggccgatcat ctgcatagcg
      361 cattccaggc cgacttgaaa aatggggttt cggcgccaag tcgtgaaaaa tgcacccgcg
      421 acctggcgtt tgttctcaat gccctggctg atggcctgaa gcgactcccg gcccccgctg
      481 ccctgctcaa tgtccatcgc tatctgaccg aacaggtgtc accctttatc gcttacgggg
      541 aagacgtctg gaaaatgacc tggcaggccg cctggtttca tgccgaaaag attttcccac
      601 ccgcaaccta tcgcgaactc aaaacgtggt tccgcagcct ggaaaaaatc ggcaaaccca
      661 tggtgctgat tggcgcttta aatcagaaat ttgtctgtag ccaggagccc attttcagcc
      721 cgaacccgga cgaagaaact gaatggcggc aaacggtgtg cacactactg gcactggctc
      781 agatcaacgc tgcttcacca aagagtttat cagcgattcg atggatactg gccgagtcgc
      841 tgccactgac caaaagtcag gattttcctg aaaagttggc cctgattctc acaagtcttc
      901 gcaactggtt tattgacctt gatctgtctc cgattcaggc gcaattcgag gcatttgaac
      961 aggttctggc tgatctgggg acgctcagga aattttcact ggccgtcaat gatgttcgcc
     1021 agaaattaac tgagcgtctg ccagccgttc aggaacttgg cagtcgcgag cgggtcaatt
     1081 cctttttgtt ctggatggga cgcaatgccc tctatggaag tgccgaaccc gccagccgct
     1141 ggggtgtgcc accggaattc aaatttcttt cagagagcgg gtttttgaaa ctggggacgc
     1201 tcacaaccag tgaaatcgag cggttgcagg ccgcgttgtt gaattcgttt gccgtgcatc
     1261 tgcctcgcat ttcagaactg gcaccaacca ttcagcggct ccagcaactg gctcaaactg
     1321 aaaaagattt gcggcaaatc tggagccggc agtggcaggt tgaaggtaaa gaatttagcc
     1381 atgcttcgtg ccagcgggat ctgcaatgga tggcccgccg ggtctgcatt gatggctggc
     1441 ttggtcatcg aaccgcagag ggcgccattg cctggtatct taatgaagtc attccgtttg
     1501 ctggccagat tacgccgaaa aactatatca aggcgatgaa gctgctgttt gaaaaattcc
     1561 agaccagcaa cctggtattg ccagatatga aaaccgcggc tgacaatttc caaacttcgg
     1621 tttcaaccac gctttcatca cccaaaaaag tggatccaac cgttctcaaa gaatttcaac
     1681 caccagaaga cgaaccacag agtttgtgga agaaaatttc tggtgccttt tcgcggccca
     1741 ggcttgatgc gtaaggctgc gcgggctaag ggctaaggg
//
LOCUS       DAOQDO010001523         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70125_length_1779_cov_2.262181, whole genome shotgun sequence.
ACCESSION   DAOQDO010001523 DAOQDO010000000
VERSION     DAOQDO010001523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70125_length_1779_cov_2.262181"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1779)
                     /locus_tag="PKZ53_20410"
     CDS             complement(<1..>1779)
                     /locus_tag="PKZ53_20410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HNJ42857.1"
                     /translation="SSSVLALAVQPDGKILIGGSFVLINGVVSPGLARLNPDGTLDPT
                     FTSPQSLSVFAIALQPDGKIVIGGDFTFVAEQPAQRIVRLNPDGTRDTTFNSSANDLI
                     WTLGLQADGKLLVGGDFSMLGGQPCSRIGRLNADGTLDTSFPGGADSTVKTVLIQPDG
                     KIVVGGAFTMLGGQPRSRIGRLNADGTVDPVFNPGADGTVISLALQTNGKILAGGEPF
                     FTLGGQPRNSIGRLNADGTIDPTFNPGAGSLNSIAIQTDGKIVVGGSFFSLGGQSRIF
                     IGRVTNPDAAVENLSVDPFGTTLTWQRSGSSPEVQRVLFEQSTDGVNFTPLGNGTRIA
                     GGWQLTGLTIPFEQNLFFRARGVGSGLSSGSHSLYESVTNIFLPCALPATVSGSGAIC
                     LGTPSLITVTVSGGVGPYTVTLNNGGGTQTGNGPVFNFPVSPAETTTYSVQSGSDSTG
                     CAIFGTSSATVTVTPVASVNPVTPPAICGGTPTSIPLTSTPGGATLSWMIGPVTGTVT
                     GQAAGSGSTISQTLNGSGTVTYLVTATLDGCPGPTTSIVQTVEPRATANAGPDQTVCA
                     SSPAVTLAGVIGGSASNGTWSGGSGS"
BASE COUNT          404 a          518 c          489 g          368 t
ORIGIN      
        1 aagctgccgc taccaccgct ccaggtaccg ttgctggcgc tcccgccaat cacaccggcc
       61 agcgtgacgg ctgggctact ggcacagacg gtctggtctg gtccggcatt ggccgtggcc
      121 cgaggctcga cggtttgcac aatgctggtc gttgggcctg gacatccatc cagggttgcg
      181 gtgaccagat aggtgaccgt tccactgccg ttgagtgttt gtgaaatcgt gctgccggaa
      241 cccgctgcct gaccagtgac agtgccggtc acgggtccaa tcatccagct caacgtcgcc
      301 cctcccggcg tactggtcag aggaatactg gttggcgtcc ctccacaaat cgcaggcgga
      361 gtaaccggat tgacggatgc cactggtgtc accgtaactg tcgcgctact ggtcccaaag
      421 atggcacatc cggttgagtc acttccggat tgaactgaat aggttgtggt ttctgccggg
      481 ctcactggaa aattaaaaac cggcccgttc ccggtctggg tgccgccgcc attgttgagg
      541 gtcacggtat aggggccgac gcctccggaa accgtgacag tgatgagcga aggagttcca
      601 aggcaaatgg cgccactgcc actgaccgtt gccggcagag cacaggggag aaagatattg
      661 gtcactgatt catacagtga atgcgaacct gaagaaagac ctgaccctac accacgcgcc
      721 cggaagaaaa gattctgttc aaaaggaatg gtcaatcccg taagttgcca gccaccggca
      781 atgcgggtgc catttccaag gggtgtgaaa ttgacgccat cggtggattg ttcaaagaga
      841 acccgctgga cttcagggct gctgccgctc cgttgccagg tcagtgtggt gccgaaagga
      901 tcaacggata aattttctac cgccgcatcc gggttggtca cccgaccaat aaagatccgg
      961 ctttgccccc cgagggagaa gaacgacccg ccaaccacaa ttttgccatc ggtctgaatc
     1021 gcgatggagt tgagactgcc ggcaccggga ttaaacgttg gatcaatggt tccatccgcg
     1081 ttcaaccggc ctatgctatt tcggggttgc cctcccaaag tgaaaaaggg ttcccctccg
     1141 gccagaattt tcccattggt ctgaagtgcc agtgagataa ccgtaccgtc ggcaccagga
     1201 ttaaacaccg ggtcaacagt tccatccgca ttcaaccggc caatccggct gcgcggctgc
     1261 ccaccgagca tagtgaaagc tccacccacc acaattttcc catctggctg gatcaacacc
     1321 gttttgactg tactatcggc acctccgggg aacgatgtat ccagggttcc atccgcgttc
     1381 aaccggccaa tccggctgca cggctgcccg cctagcatgg aaaagtctcc cccaacgagc
     1441 aattttccat ccgcctgaag gcccagtgtc caaataaggt cattggcact ggagttaaac
     1501 gtcgtatcgc gtgtcccgtc agggtttaat cggactatcc gttgagctgg ttgctcagcg
     1561 acaaaggtga aatctccccc aatcacaatt ttcccatctg gctgaagcgc gatggcaaag
     1621 acgctcagcg attgagggct ggtaaatgtc ggatccagcg ttccatcggg attcaaccgc
     1681 gcaaggccgg gggaaacgac accattgata agtacaaagc ttccgccaat caggattttt
     1741 ccatctggct gcactgccag cgcaagaact gaactagac
//
LOCUS       DAOQDO010001524         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70156_length_1778_cov_3.673244, whole genome shotgun sequence.
ACCESSION   DAOQDO010001524 DAOQDO010000000
VERSION     DAOQDO010001524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70156_length_1778_cov_3.673244"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..>1778
                     /locus_tag="PKZ53_20415"
     CDS             255..>1778
                     /locus_tag="PKZ53_20415"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNJ42858.1"
                     /translation="MADELPLYPDEFDESEPQKAQDLLKNEYLIRSELNLERWPGIWQ
                     AANKTTEFNEIRTIERKIKLNNKEILAKVTIVPDLQFGTLNTEDQKTLYALLSIWEKK
                     GRPDKIHFTIRGILKALDRQYGKIAREGIINSLKRLKAVDITWENSFFDKTQNKTIKQ
                     LAMFNIISFLSLTSEESEESVEDEITVTFHELIHSNLKSYHSIPTKYHVLIGIKNPLA
                     QLVYKYIEPRLYSATRFERRTKDLLMDLAIQSTRYHKLSNRVDALNRAIRELQGLKIQ
                     NGVLSVYLEPSKDNDYKLVAIKTKNQADQIPEMAADATERTVHSENETNIIIENKSFV
                     NKIQIDQELEIFELFRRKFKLARKRPTLKEFEKAKHWIEIFDGSLQTFERFIEFAEAE
                     AQKTSFKIQNLAGLEQYFDQFINPSPSAKDPDPSVNQEVLVDADPAPPVSIHTEKEKA
                     FQCLRLMSETEQAYWCQSAKSFLHRSEPATLKKILTFGPEVQKETLLAVAARQFAAAI
                     "
BASE COUNT          569 a          429 c          369 g          411 t
ORIGIN      
        1 gacccggacc caagtggggt aggacccgga cccaagtggg gtaggacccg gacccaagtg
       61 gggtaggacc cggacccgcc attcccacaa actacagaaa aaaaagactt tacatagcgt
      121 caaaaattct ctaacatgta ttaagactta tttaagactt atttaagaca caatacatgc
      181 gcgcgtacgc gcgcgagtgt gtgtttttca ctttttttgt gtttttgatc caaaccaggt
      241 tttgagcacc ctgtatggct gatgaactgc ccctctaccc tgacgaattt gacgaatccg
      301 agccgcaaaa agcccaggat ttgctcaaaa acgaatacct gattcgttca gaactcaacc
      361 tggaacgctg gcctggtatc tggcaggcgg ccaacaaaac taccgaattt aacgaaatcc
      421 ggaccattga gcgaaaaatc aagctcaaca acaaagaaat cctggccaaa gtgaccattg
      481 tccctgacct gcagtttgga acactcaaca ccgaggacca gaaaaccctc tacgctttgc
      541 tctcgatttg ggaaaaaaag ggccgacctg acaaaatcca ctttaccatc cggggaatct
      601 taaaagccct tgatcggcaa tacggcaaaa ttgcccgtga aggaatcatc aactcactca
      661 agcgactcaa agcggttgat atcacctggg aaaattcgtt ctttgacaaa acccagaaca
      721 aaaccatcaa gcaactggca atgttcaaca tcatttcgtt tttgtcgctg acgtcggaag
      781 aatccgaaga atcggttgaa gacgaaatca cagttacttt ccacgaacta atccactcaa
      841 acctcaagag ttaccactcg attcctacga aataccacgt gttgattgga atcaaaaacc
      901 ctctcgctca actggtttac aagtacatcg aacccagatt gtactctgcc actcgatttg
      961 aacggcggac caaagatctg ctcatggatc tggcgattca atccacccgg taccacaaac
     1021 tttcaaaccg ggtggatgcg ctgaaccggg ccattcgcga actgcaaggg ctcaaaatcc
     1081 aaaatggcgt gctctcggta taccttgagc ccagcaaaga taacgattac aagctggtgg
     1141 caattaaaac taaaaatcaa gctgatcaaa ttcccgaaat ggcagcggat gcaaccgaaa
     1201 gaacagtaca tagtgaaaat gaaacaaata ttataattga aaacaaatct ttcgtaaata
     1261 agattcagat tgatcaggaa ctcgaaattt ttgagctatt tcgaagaaag tttaaacttg
     1321 ccagaaaacg accaacactc aaggaatttg agaaagcgaa acactggatt gagatatttg
     1381 atggatcatt acaaacattt gaaagattta ttgaattcgc tgaggcggaa gcccaaaaaa
     1441 ccagctttaa aattcaaaat ctggctggtc ttgagcaata ttttgaccag tttattaatc
     1501 cgtctccgtc tgcaaaagat ccagatccat cagtcaatca ggaagtcctg gtggatgctg
     1561 acccggctcc gcctgtcagc attcacaccg aaaaagagaa ggcttttcaa tgtctgagat
     1621 tgatgtctga aaccgagcag gcctactggt gtcagtcagc aaaatctttc ctgcatcgat
     1681 cagagccagc tactttaaag aaaattctga cttttggacc cgaagtgcaa aaggaaacct
     1741 tgctggccgt ggctgccagg caatttgctg ctgcgatc
//
LOCUS       DAOQDO010001525         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70241_length_1777_cov_3.360046, whole genome shotgun sequence.
ACCESSION   DAOQDO010001525 DAOQDO010000000
VERSION     DAOQDO010001525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70241_length_1777_cov_3.360046"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..627)
                     /locus_tag="PKZ53_20420"
     CDS             complement(49..627)
                     /locus_tag="PKZ53_20420"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ42859.1"
                     /translation="MSLKKLYLTLGLVLAVGMQVAGTVPAKADGRRVAGVAPVAAKDL
                     IANAAKEAKASNKAVFVKFTASWCIWCRRLNAALGLQEIQKPFGDNFVYLEIDVLERG
                     EKEALENPGGEDYLKQWGGENAGLPFSVILDKNGKKIADSNLMPNQKNIGYPGSDEEI
                     EAFLKLLKKGAPRLTEAQQLQISSTLKKIAPK"
     gene            complement(689..>1777)
                     /locus_tag="PKZ53_20425"
     CDS             complement(689..>1777)
                     /locus_tag="PKZ53_20425"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNJ42860.1"
                     /translation="SSVPAQSPEPPTPPAEEKPLVLLSVHKSDKPEPVAFVSVTKSIP
                     PENSALSTDVQVAETVESTYRKKVSWVRVATLIVVLGAGLLAFGFWWLKNSVPEVPVA
                     KIPAKSQPASKSTPSTPAPTKSSTPDPPPSPPPAGAALIVAADGSGAFKRINDALAKA
                     KPGERILVKAGTYRETIVLDKAVELVAEGSPDQTVIESTSGPVMVVQVDKAKIQGFTL
                     NGKAGQSNGGASTVEIKQGKATLDNCRISSDSLVCVAIYGRETTPVLQNCTVSNGKAS
                     GIGAWDGAQGMILNCDISSNRWAGVVVTKGSRLTIKDSRINKNGLQGIKVDSGGSATV
                     EKCDLSKNRKGAWSISSDATVEKLDNKE"
BASE COUNT          399 a          482 c          397 g          499 t
ORIGIN      
        1 ttcattcttc attcttcatt cttcattctt cattcttcat tcttcattct acttcggggc
       61 aatttttttc aacgtgctgg aaatctgtaa ctgttgggct tcggttaaac gcggtgcacc
      121 ttttttgagc aacttcagaa aagcctcgat ttcttcatca ctaccaggat acccaatgtt
      181 tttctggttg ggcatcagat ttgagtcggc tattttcttt ccatttttgt ccaaaatgac
      241 gctaaacggg agccccgcat tctctccgcc ccattgtttc aaatagtctt caccgccagg
      301 gttttcaagt gcttcttttt cgccacgctc cagaacatca atttcaagat aaacaaaatt
      361 gtcgccaaac ggcttctgaa tttcttgtaa tcccagcgcc gcatttaacc gacgacacca
      421 gatacaccac gacgccgtga actttacaaa taccgctttg tttgaagctt tggcttcttt
      481 ggcggcattg gcaatcaagt ctttggcagc gaccggagcc acgccagcaa cacgtcgtcc
      541 atctgctttt gctggaacag ttccggcaac ctgcatccca actgccagca ccagcccaag
      601 tgtaagataa agttttttca atgacataga tcaaccttcc ttgtgctttg acgaatcgca
      661 ggtaatagct cgtgactcgc tgttacggct attccttatt atcaagcttc tcaaccgtgg
      721 catcgcttga aattgaccag gcgccttttc ggtttttcga aagatcacat ttttcaaccg
      781 tagccgatcc accactatcc accttgatac cctggagtcc atttttgtta attcggctat
      841 ctttgatggt caaacgactg cctttggtaa ccacaacacc cgcccagcga ttgctggaaa
      901 tatcacaatt gaggatcatt ccctgtgccc cgtcccaggc accaatccca ctcgctttgc
      961 cattggaaac ggtacagttt tgcaaaaccg gagtggtttc acggccataa atcgccacac
     1021 acaccagtga atccgagctt atccggcagt tgtcgagggt tgctttgccc tgtttgattt
     1081 caaccgtcga ggcgccccca ttgctttgac cagcttttcc attgagcgta aagccttgaa
     1141 tttttgcttt atcaacctgg actaccatca cggggccgct ggtactttca atgactgtct
     1201 ggtcagggct gccttcagcc accaattcaa ccgctttatc aagcacgatg gtttcacgat
     1261 aggttccggc tttgaccagg attcgttccc ccggtttggc tttggccagc gcgtcattga
     1321 tccgcttaaa cgccccggac ccatcagccg ccacaatgag tgctgccccc gccggtggcg
     1381 gagatggcgg cggatcaggg gttgaagatt tcgtaggtgc cggagttgac ggcgtgcttt
     1441 ttgaagccgg ttgggatttg gctggaattt tggccaccgg gacttcaggc actgaattct
     1501 ttaaccacca gaaaccaaag gccagtagcc cggcacccaa aaccacaatc aaggttgcca
     1561 cccgaaccca tgaaaccttt ttgcgatagg tggattcgac ggtttcggcc acctgaacat
     1621 cagtcgagag ggctgaattt tccggtggaa ttgatttggt cactgaaaca aacgcaaccg
     1681 gttcgggctt gtcactttta tgaaccgaaa gcagaaccag tggtttttct tcagcgggcg
     1741 gggtcggagg ttcaggtgat tgagccggaa ctgatga
//
LOCUS       DAOQDO010001526         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70244_length_1777_cov_3.156214, whole genome shotgun sequence.
ACCESSION   DAOQDO010001526 DAOQDO010000000
VERSION     DAOQDO010001526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70244_length_1777_cov_3.156214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1777
                     /gene="uvrB"
                     /locus_tag="PKZ53_20430"
     CDS             59..>1777
                     /gene="uvrB"
                     /locus_tag="PKZ53_20430"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNJ42861.1"
                     /translation="MKFELVSPYKPTGDQPQAIETLVRNVQAGSPSQVLLGITGSGKT
                     FTIANMIEQLQRPTLVMAHNKTLAAQLFQEFRGFFPKNAVEYFVSYYDYYQPEAYVAS
                     TDTYIEKEATINEEIDRLRLSATRSAFERRDFVIVSSVSSIYGIGDPDAYFGMSMFIE
                     PGQVLKRQELLKRLVELQYDRNDVDFGRGSFRVRGDVVEIYPSYSEQAIRVSFWGDEI
                     DTMNVIDPLLGEILEPINRRYPIYPKSHYVMPKDVLKRAVVTIKEELETWEPQLLEMG
                     KLVEAQRLRQRTIYDLEMIRELGFCRGIENYSRHLTGRPAGEPPPTLFDYLPEDTLIV
                     VDESHQSIPQIRGMYEGDRSRKSTLVEYGFRLPSAMDNRPLKFAEWEKRIGQTIFVSA
                     TPGPYELQKTGGEIIEQIIRPTGLLDPLVDVRPVRGQIDDLLHEIRLRTAKKQRVIVT
                     TLTKKMSEDLTDYYLDVGVNVRYLHSEIETLERIKILRDLRRGDFDVLIGINLLREGM
                     DLPEVSLVAILDADKEGFLRSESSLIQIIGRAARHSEGKAILYADKMTDSMRRALREM
                     RWADRTK"
BASE COUNT          443 a          433 c          459 g          442 t
ORIGIN      
        1 ccttcgtggt ttacccctga atcacatttt ttgacgatgc tttgcaggaa gtcgctcgat
       61 gaaatttgaa cttgtttcgc cttataaacc aaccggagat caaccgcagg cgattgaaac
      121 tctggttcgc aatgttcaag ccgggagtcc atctcaagtc ttactcggca ttactggcag
      181 tggtaaaacc ttcacgattg ccaacatgat cgaacaactc cagcgcccaa cgctggttat
      241 ggctcacaac aaaaccctgg cagcacaact ctttcaggaa tttcggggat tttttcctaa
      301 aaacgcggtt gaatactttg tcagctacta tgactactat caaccggaag cctatgtcgc
      361 ctcgacggat acctacattg aaaaagaagc caccatcaac gaagaaattg accggcttcg
      421 actctcagcc acccggtcgg cctttgaacg ccgggatttt gtgattgtct cttcagtttc
      481 atcaatttac gggattggcg acccggatgc ctactttgga atgtcaatgt ttattgagcc
      541 gggtcaggtc ttaaagcgcc aggaactcct caaacgactg gttgaactcc agtatgaccg
      601 caacgatgtt gattttggcc ggggttcgtt tcgagttcga ggcgatgtgg tcgaaattta
      661 tccgagctat tcggaacagg cgattcgggt ctcgttttgg ggtgacgaaa ttgacacgat
      721 gaatgtgatt gacccgttac tgggtgagat cctggaacca atcaatcgcc gctatccgat
      781 ttatccgaaa tcgcactacg tcatgcccaa agatgtgctc aagcgggccg tcgtgacaat
      841 caaggaagaa cttgaaacct gggaaccaca actgctggaa atgggcaagc tggttgaggc
      901 gcaacgcctc cggcaacgca cgatttatga tctggaaatg attcgcgaac tgggcttttg
      961 tcgcggcatt gaaaactact cacggcattt gaccggtcgc ccagccggtg agccgcctcc
     1021 gacgctgttt gattatttgc cggaagatac cctgattgtg gtggatgaaa gccaccagtc
     1081 aattccgcag attcgtggga tgtatgaagg cgaccgctca cgcaaatcaa ccctggtcga
     1141 atatggcttt cgattgccgt cagcgatgga taaccgtccg ctcaagtttg cagaatggga
     1201 aaaacgaatt ggccagacaa tttttgtgtc ggccacgccc ggtccctacg aactacaaaa
     1261 aactggtggt gaaatcatcg agcagatcat ccgtccaacc ggattgcttg atccactggt
     1321 tgatgtccgc ccggtgcgcg gtcagataga tgacttgctg cacgaaatca ggttgcgaac
     1381 ggctaaaaaa cagcgcgtga ttgtgaccac gctgaccaaa aagatgtcag aggacctgac
     1441 cgattactac cttgatgtcg gggtcaatgt ccgctatctc cattcggaaa ttgagacgtt
     1501 ggagcgcatc aagattctga gggacctgcg acgcggggat ttcgatgtat tgattggaat
     1561 taatttgctg cgtgaaggga tggatttgcc tgaagtttcg ctggtcgcga ttctcgatgc
     1621 cgacaaagaa ggctttttac gcagtgagtc atcactgatt cagattattg gccgtgccgc
     1681 tcgccattct gaaggcaagg caatcttgta tgctgacaag atgactgatt cgatgcgccg
     1741 cgcgcttcga gaaatgcgct gggctgatcg aaccaag
//
LOCUS       DAOQDO010001527         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70270_length_1777_cov_2.235192, whole genome shotgun sequence.
ACCESSION   DAOQDO010001527 DAOQDO010000000
VERSION     DAOQDO010001527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70270_length_1777_cov_2.235192"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1531)
                     /locus_tag="PKZ53_20435"
     CDS             complement(59..1531)
                     /locus_tag="PKZ53_20435"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HNJ42862.1"
                     /translation="MTTPRKTYKLFINGEFVDAVDGKTFPDYNPATGQVTANVSEAGS
                     NDVCWAVESAQAALKGPWGRMAMTDRLNLLRKVADGIEARFNDFVDAEIADTGKPRSL
                     ACAVDIPRGAANFRIFAEIARSFATECFEMDTEDGAGAINYSLRVPLGVVGVICPWNL
                     PLLLMTWKVAPALAAGNTVVVKPSEETPGTATLLGEVMKQVGVPPGVFNVVHGFGPNS
                     AGQAITEHPGIRAVTFTGETRTGQAIMKASSVNMKQVSFELGGKNPAIIFADADFDKA
                     VAGTARSVFSNCGQVCLCSERVYVERPIFTRFVEAMKVEARKLRPGDPYDPATTIGPL
                     ISDTHRQKVLSYYQLAREEGAEIVTGGGIPNLGEPFNGGYFIQPTIFTGLHEHARVVK
                     EEIFGPVCHICPFDSEEEAVVLANNTDYGLCAAVWTQNLTRAHRVARQLECGIVWINA
                     WFLRDLRTPFGGMKMSGIGREGGLHSIQFYSELKNVCVKL"
     gene            complement(1546..>1777)
                     /locus_tag="PKZ53_20440"
     CDS             complement(1546..>1777)
                     /locus_tag="PKZ53_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42863.1"
                     /translation="LLFGTDAPSVDHPAASPIHACAHGGLWTGRVAILEGLESNFLPL
                     DLRLDGVLHILWNPMQLHADAKGALVTFYPSH"
BASE COUNT          408 a          489 c          483 g          397 t
ORIGIN      
        1 gtgggttgta ttcttccaat ccccacccgc tcacgcaggt ggtactgact cttattcttc
       61 agagtttgac gcacacgttt tttaattccg aataaaactg gattgaatgc aggccgcctt
      121 cacgaccaat cccactcatc ttcatgccgc cgaagggtgt tcgaagatcg cgtaaaaacc
      181 aggcgttgat ccagacaatg ccgcattcga gttgcctggc gacgcgatgc gcacgggtca
      241 ggttctgtgt ccagaccgcc gcgcacaggc cataatcggt gttgttggcg agcacgacgg
      301 cttcttcttc gctatcgaag gggcaaatgt gacagaccgg accaaagatt tcttctttaa
      361 ccacgcgagc gtgttcatgg aggccggtaa agatcgtcgg ctggatgaaa tagccgccgt
      421 taaatggctc accgagattc ggaatgccgc cgccggtgac aatttcggcg ccctcttctc
      481 gggcaagttg atagtaggaa agaacttttt gccgatgcgt gtcggaaatc aatgggccga
      541 tggtggttgc cggatcatac ggatcgcctg gtctgagttt acgggcttcg accttcatcg
      601 cttcgacaaa ccgggtgaaa atcggacgtt caacataaac tcgctcagaa cacaaacaga
      661 cctgcccgca atttgagaaa accgagcggg ctgttcccgc gacagctttg tcaaaatcgg
      721 catctgcaaa gataatggcc gggttttttc cgccgagttc aaaggaaacc tgcttcatat
      781 tgactgatga ggctttcata attgcctgac cggtgcgggt ctcgccggta aaggtcaccg
      841 cacggatgcc tggatgctcg gtaatggcct gtccggcgga atttgggcca aatccatgaa
      901 ccacattgaa gacgccgggt gggacgccga cctgtttcat cacttcaccc agcagtgtgg
      961 ccgtgcctgg ggtttcttct gaaggtttta cgacgaccgt gttgccagcg gccagtgctg
     1021 gtgccacttt ccaggtcatc aacagaagcg gcagattcca gggacaaatc acgccgacca
     1081 cgcccagtgg tacacggaga ctgtaattga tggcaccggc gccatcttcg gtatccatct
     1141 caaaacattc cgtcgcaaag ctgcgggcaa tttcggcaaa gatccggaag ttggctgctc
     1201 cacgcggaat atcaaccgca caggcgagac ttcgcggctt tccagtatcg gcaatctcag
     1261 cgtccacaaa atcattgaag cgggcttcga taccatcggc cactttgcgg agcaggttca
     1321 ggcggtcagt catagccatc cgtccccagg ggcctttaag cgctgcctgg gctgattcaa
     1381 ccgcccaaca gacatcgttt gatccggctt cgcttacgtt ggcggtgacc tgcccggtgg
     1441 ccgggttata gtcaggaaag gttttgccgt caactgcatc aacaaactct ccgttaataa
     1501 acagcttgta ggttttccgt ggtgtggtca tggcgagtgg tccaatcaat gtgaaggata
     1561 aaacgtgaca agcgcgcctt tggcatcggc gtgaagttgc attggattcc acagaatatg
     1621 cagaacccca tccagccgca aatccaaagg caggaaattg ctttcgagtc cttcaaggat
     1681 cgcgacccgt ccggtccaca gcccgccgtg ggcacaagcg tgaatcggag acgccgccgg
     1741 atggtccacc gacggcgcat cggttccaaa caaaagc
//
LOCUS       DAOQDO010001528         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70373_length_1775_cov_3.812791, whole genome shotgun sequence.
ACCESSION   DAOQDO010001528 DAOQDO010000000
VERSION     DAOQDO010001528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70373_length_1775_cov_3.812791"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..784)
                     /locus_tag="PKZ53_20445"
     CDS             complement(50..784)
                     /locus_tag="PKZ53_20445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ42864.1"
                     /translation="MRTLLCAGMCVVVVLVGCGGSGMAQNQKLQKRQPPAEKAAPPAQ
                     QPPSKKPVKKNPAPPPALTKPKPSLVEDLSPKQLENLQAVIETSMGNITLEFFGQVAP
                     NHVRQFLRLADQGYYNGTTFSRIVKGFVIQGGNAGTWLDTNPNQKVDFETPTLKGEFS
                     VIPHERGTLSMARAGDDENSATTHFFICLGRQSSLDGKYSAFGRVIEGMDVVDAIAAV
                     KIKGGTEEPEDRVEVKTIRVIFPPEK"
     gene            1063..>1775
                     /gene="argS"
                     /locus_tag="PKZ53_20450"
     CDS             1063..>1775
                     /gene="argS"
                     /locus_tag="PKZ53_20450"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNJ42865.1"
                     /translation="MFLHIQEKLRAKLSDSIQKHFHLNLPTIVLEHPPKLELGDLAAP
                     LAFELAKQLKQATGTKHNPRQLAEQLKADVLDLPELDRVEVAGAGYLNFFLKRDEVFL
                     CSLSPDGEPPLQPQIGGKLIVEHTSVNPNKAAHIGHIRNAVLGDTLVRLLKACGEDVE
                     TQNYIDNTGVQVVDVVIGFVFLEEKSLADIQQIEGKFDYYCWDLYARVGQFYEADPER
                     KKLREQVLHEIESGQGEIAG"
BASE COUNT          404 a          497 c          427 g          447 t
ORIGIN      
        1 caattcttca ttcttcattc ttcattcttc attctcaatt cttcattctt catttttccg
       61 gtgggaaaat gacccggatg gtctttacct caacccggtc ttcgggctct tcagtaccgc
      121 ctttgatttt gacagcggca atggcatcaa ccacatccat tccttcaatc acccgtccaa
      181 acgcgctgta ttttccatcg agcgatgatt gtcggcccag acagatgaaa aaatgggtgg
      241 tggcactatt ttcgtcatca cccgcccgag ccatcgaaag cgtgcctcgt tcgtggggaa
      301 tcacactgaa ttcccctttc agagttggtg tttcaaagtc caccttctga tttgggttgg
      361 tatcaagcca cgttccagca tttcctccct gaatcacaaa ccctttgaca atccggctaa
      421 aggttgtccc gttgtaatac ccctggtcag ccagccgcaa aaactggcgg acgtggttgg
      481 gtgctacctg tccaaaaaac tcaagcgtga tgttccccat tgaggtttca atcaccgcct
      541 gcaggttttc aagctgcttt gggctaagat cttcgaccag gcttggcttt ggcttcgtca
      601 gtgctggcgg tggagcagga ttttttttca ccggtttctt tgacggcggc tgttgcgccg
      661 gcggtgcggc cttttcggct ggcggctggc gcttttgcaa tttctggttt tgcgccatcc
      721 ctgatccacc acatccaacc aacacgacaa cgacacacat tcctgcacaa agtagcgttc
      781 gcataatttc aacctctgag caacataata gttaggacgg ctacgatgtg acgtagcgtt
      841 tgaaaagtga ctggtacaca tttaagagag ttgaacttgc ggcgtcaagt tgccagattg
      901 caaagccgcg tcaagggcgc cgcgctccac atttgcttgt tcgccactcc caagtaggct
      961 ataaaagcgg gctctcatca ccaaagcggg aaccacagtc attgtggacg atttgccgct
     1021 tgctattcgc tattcgttct tcgctcttca ctcaaaatac ctatgttctt gcatatccag
     1081 gaaaaactgc gcgccaagct ttcagatagc atccagaaac actttcacct gaacctgccg
     1141 accatcgtgc tggagcatcc gcccaagctc gaactgggtg atctggccgc gccgctggcg
     1201 tttgaactcg ccaaacaact caagcaggcc actggcacca agcataaccc gcgtcaactc
     1261 gccgaacagc ttaaagccga tgtgctggac ctgcccgaac ttgaccgggt tgaagtcgcc
     1321 ggggccgggt atctcaattt ctttctcaag cgcgatgaag tgtttttgtg cagcctctct
     1381 ccagacggag agccgccgct ccagccacaa atcggcggga aactcatcgt cgaacacacc
     1441 agtgtcaacc ccaataaagc cgctcacatt ggtcacatcc gcaatgcggt gcttggcgac
     1501 acgctcgttc ggctgctgaa agcctgcggt gaagacgtcg aaacccagaa ttacattgat
     1561 aataccggcg tgcaggtggt ggatgtcgtc atcggctttg tgtttctgga agaaaaatcg
     1621 ctggcggata ttcagcaaat cgaaggcaaa tttgattact actgctggga cctctatgcc
     1681 cgggttggcc agttttacga agctgacccc gaacgaaaaa agctgcgtga acaggtgctc
     1741 cacgaaatcg aatccggcca gggtgagatt gccgg
//
LOCUS       DAOQDO010001529         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70466_length_1774_cov_2.599767, whole genome shotgun sequence.
ACCESSION   DAOQDO010001529 DAOQDO010000000
VERSION     DAOQDO010001529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70466_length_1774_cov_2.599767"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..>1774)
                     /locus_tag="PKZ53_20455"
     CDS             complement(130..>1774)
                     /locus_tag="PKZ53_20455"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ42866.1"
                     /translation="GTSNGFNRFVPDQQKFVRYTEKNGLPAPYVLEILPDPGGDLWIS
                     FHFVLAKFEPSTGTFHVYDSKDGIQTRRFNIGCAFQAPDGQFYLGGVGGITRFWPDKI
                     KDNPEIPPVRLTAFRKFDKVTRLNASLCTLQEIELKYWETVFGFEFSALNYRSPEKNQ
                     YAYQLVNFDADWVYCGNRRFVNYTNLNPGEYIFRVKGSNNDGVWNDVGQSIRIRIIPP
                     PWRTWWAYGLYLLGFSAGLWGVVQFQTSKVKSRAHLKEIHLRAKTAESEAEVSRAKAE
                     AAVNLARAIEAERQRQAQELEEARQLQLSMLPRTIPNLPDLAISVYMKTATEVGGDYY
                     DFHLADDQTLSVVIGDATGHGLRAGMMVTATKSLFGTLAQEPDLPQLLSRMSQALKRL
                     NLKTLYMALTVLRIKANRVTLGAAGMPPALVYRAHTGQIEDIEIEGLPLGSFIQFPYQ
                     TAEFSLDPGDMVVLMSDGFPEQLNPTEEVFGYGRMREVLEASPAQSPQDVISRLIQTG
                     EAWARGVPQADDMTFVVIQYQPVRSVRQSSSVRVETVSG"
BASE COUNT          433 a          490 c          426 g          425 t
ORIGIN      
        1 aagggatgag ggatgaagga tgagggatga aataaaacca attcttcagc ccgacgtctt
       61 cagcccgacg tcttcagccc gttttcttca gcccgacgtc ttcagcccga cgtcttcagc
      121 ccgctttctt caacctgaca ccgtttcgac ccggactgaa gaagattgtc tgactgatcg
      181 aaccggctgg tattgaatca ctacaaacgt catatcatca gcctggggaa cgcctcgggc
      241 ccaggcttca ccggtttgaa tcagccggga aatcacatcc tgcggtgatt gggcaggtga
      301 agcctccaaa acttcccgca tgcggccata gccaaaaact tcttcagtcg gattgagttg
      361 ctctggaaat ccatcgctca tcagcacaac catgtctcct ggatccaggc taaattcagc
      421 ggtttgatat ggaaactgga taaaactccc gagtggaagc ccttcgattt caatgtcttc
      481 aatctggccg gtatgagcgc gatacaccag cgccggtggc atcccggcgg ctccaagcgt
      541 tacccgattc gccttaatcc tgagcacagt cagcgccata tacagcgttt ttaaattcaa
      601 tcgcttgagc gcctgggaca ttcgcgagag caactgtggc aaatcaggtt cctgagccag
      661 cgtcccaaac aaacttttgg tcgcggtcac catcatccca gcccgcaacc cgtggccagt
      721 ggcgtcgccg atcacaactg acagagtctg gtcatctgcc agatgaaaat cataataatc
      781 gccgccaact tcggtggctg ttttcatata aaccgaaatt gcaagatctg gaaggtttgg
      841 aatggttcgc ggcagcatcg aaagctgcaa ctgacgggct tcttccagtt cctgggcctg
      901 ccgttggcgc tcggcttcaa tcgcccgggc cagattgact gccgcctcag ctttcgcccg
      961 tgaaacttca gcttcgcttt cagccgtttt cgcccgtaga tgaatttctt tgagatgagc
     1021 gcgcgatttc accttgctgg tttgaaattg aaccacaccc cacaggccag cactgaatcc
     1081 aagcaggtac aatccataag cccaccacgt ccgccacggc ggaggaataa tccgaatccg
     1141 aatcgactgg ccgacatcgt tccagacccc atcattgttt gatcctttga cccgaaagat
     1201 atattcgcca gggttgagat tggtgtaatt gacaaagcgg cgattgccac agtacaccca
     1261 atcggcatcg aaattgacca actggtatgc atattgattt ttctcggggg accggtaatt
     1321 cagtgccgaa aattcaaacc cgaagactgt ttcccaatac ttcagttcga tttcttgcag
     1381 cgtacacagc gacgcattga gccgggtgac tttgtcaaat ttgcggaaag cagtcagccg
     1441 gacaggtgga atttcaggat tgtctttgat tttgtctggc caaaatcggg taatgccacc
     1501 cactccgccc aggtaaaact gaccatccgg cgcttgaaag gcacatccga tattgaaccg
     1561 ccgggtttga attccatctt tgctgtcata gacgtgaaac gttcctgtcg agggttcaaa
     1621 tttcgccagg acaaaatgaa aactgatcca gaggtctcca cctggatccg gcaagatctc
     1681 caatacatag ggcgccggga gtccattttt ttccgtgtag cggacaaatt tttgctgatc
     1741 cggcacaaac cgattgaagc cgtttgatgt ccca
//
LOCUS       DAOQDO010001530         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70588_length_1772_cov_2.552126, whole genome shotgun sequence.
ACCESSION   DAOQDO010001530 DAOQDO010000000
VERSION     DAOQDO010001530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70588_length_1772_cov_2.552126"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..529)
                     /locus_tag="PKZ53_20460"
     CDS             complement(146..529)
                     /locus_tag="PKZ53_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ42867.1"
                     /translation="MPQRFLVTKEFGALLGILSHPCRLQIVEELQDREYDVNTLQALL
                     NISHSGVSQHLTLLRAHRVVIERREGRHVYYRLRRPELATWLLDGLRFLEDEREVAEQ
                     IGSAVESVRRLWTITRNEELKPNEE"
     gene            788..1471
                     /locus_tag="PKZ53_20465"
     CDS             788..1471
                     /locus_tag="PKZ53_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42868.1"
                     /translation="MNSWTQNPEVFIEQCIAALGGHDALRGVTSIKIHACRHTHSAEN
                     PEPLISNLFTYRAVGGRIRLEEHFSDHISVVVINGLAGECATFASQSDLEANRRQTTD
                     PLHTCDVEPVKRSVRLYPRNFLAHANEHQYLPPETTVIDGRAVVWVDLPVEEVRFVFD
                     SETLLCCAVEDFRTTITTRYEAYQLANGVMTPFLEQRFRQGALIQEDQIQLLEYNLPL
                     EAQLFDIAQ"
     gene            complement(1526..>1772)
                     /locus_tag="PKZ53_20470"
     CDS             complement(1526..>1772)
                     /locus_tag="PKZ53_20470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNJ42869.1"
                     /translation="GLGLGVMLFLALQLKFTAIAQIGPRIVSLLATSQPILALIVAAL
                     VLNEWLSARQMVGVVLVVVALILVTQLKEEPVTRATE"
BASE COUNT          452 a          474 c          397 g          449 t
ORIGIN      
        1 tcgctggtca accaaatcgg tcgccaaagt gatggccaaa tgtgaaccgg ccatcacttc
       61 agccaaggcg gcatgatttc ataccccttt tatggggact tgtactaagc actaagcact
      121 aagcactaag caccaggcac atgcttcatt cttcattcgg cttgagttct tcgttcctgg
      181 tgatcgtcca gagacggcgg actgattcaa ccgcagaccc aatttgttcg gccacttcgc
      241 gttcatcttc cagaaaacgc aatccatcaa gtaaccaggt agccagttct ggacgtcgga
      301 gtcgataata gacgtgacgg ccttctcgac gttcaatcac cacccggtgg gcccgcagca
      361 acgtcagatg ctgggaaact ccggaatgac tgatattgag caatgcctga agggtattga
      421 cgtcatattc gcgatcttgc aattcttcaa cgatttgcaa gcgacagggg tgagaaagga
      481 ttcccagcaa agcaccaaat tctttagtaa ccagaaatcg ttgtggcata ttttctataa
      541 ttgatacatt tagacatttg aatgtttgaa ataaataaaa tgtctatatt gacttcgtca
      601 actgactctt ccggaaatga tggctttttt gggaactctg tggcaggttt tcctgccctg
      661 accgtgtttg tcgtaagttg ccctttccgg caccagccct cttgtttcca atagttgccg
      721 tgtgccagtc cacattccgg tgtctgatca cctcaattcc attccctctc gacccaagtt
      781 atattttatg aatagctgga cccaaaaccc tgaagtcttt attgaacaat gtattgccgc
      841 cctgggcggt cacgatgcct tgcgtggcgt caccagtatc aaaatccacg cttgccgaca
      901 tacacactct gcggaaaacc cagaaccact tatttccaat ctctttactt accgcgccgt
      961 tggtggccgg atccgacttg aagaacactt ctcagatcat atctccgtgg ttgtcatcaa
     1021 cggccttgcc ggtgaatgtg ccacttttgc cagccaatca gacctggaag caaaccgacg
     1081 acaaacgaca gaccccttgc atacctgtga tgtggaaccg gtcaagcgca gtgttcgatt
     1141 gtacccgcga aactttctgg cccacgccaa tgaacaccag tatctgccac cggaaaccac
     1201 agtgattgat gggcgcgctg tggtttgggt ggatttaccg gttgaggaag tgcgatttgt
     1261 ctttgatagc gaaacattgt tgtgctgtgc agttgaggat tttcgcacga cgatcacaac
     1321 tcgatatgaa gcatatcagc ttgcaaatgg cgtaatgaca ccatttcttg aacaacgctt
     1381 tcgtcagggc gcgctgatcc aggaagacca gattcagctt ctggaatata acctgccgct
     1441 tgaagctcaa ctctttgaca ttgcccagtg acatgctgac aaggtggcaa ctgaccagat
     1501 gattttcagt tcatttgtca aattgttatt ctgtcgccct ggtcacgggc tcttccttca
     1561 actgggttac caaaatcaac gccaccacaa ccagcacaac gccgaccatc tgacgcgccg
     1621 agagccattc attgagcacc agggcagcca caatcaaggc cagaattggc tgagacgtcg
     1681 ccaaaagcga cacaatccgt gggccaattt gggcaatagc tgtgaatttc aactgcaatg
     1741 ccagaaataa catcacccca agccccaacc cg
//
LOCUS       DAOQDO010001531         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70684_length_1771_cov_2.331002, whole genome shotgun sequence.
ACCESSION   DAOQDO010001531 DAOQDO010000000
VERSION     DAOQDO010001531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70684_length_1771_cov_2.331002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKZ53_20475"
     CDS             complement(<1..332)
                     /locus_tag="PKZ53_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42870.1"
                     /translation="MSQHTIQDTDPPPFTFTIPQTGSYTTASGTVTCNYPGYIVSTIK
                     GYMNDALVGNIPPDQTEPLDYSGETLPVTVEIQVKLKPIAPPGTTVEYSDEEDPGPEP
                     GQLLNTVS"
     gene            complement(596..>1771)
                     /locus_tag="PKZ53_20480"
     CDS             complement(596..>1771)
                     /locus_tag="PKZ53_20480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ42871.1"
                     /translation="SGFRVPGSGFRVFEFWSFSQQAVNDLSQFNHRYAAKTSTYTNFK
                     PGTRNPEPKTMFDLLKLSEMLSQISQPPEVAKTPSKVVYRENKLRVLYYLPKVKKTHS
                     VPILIVNSLINKYYILDLMPGKSYVEYLANQGFRVYMIDWGEPDDHDSHLTLEDHINK
                     YLVNITRAVLTHAGAEQVSMIGYCMGGTMALMYAALHKQFLKNLILLATPVDFHNDSL
                     LSTWAQPEYFNVDQFIDRHGNAPVEILKSTFMMLKPTKNVTKYVHLAENLHNEEYVKM
                     FQAFDYWVNDAVAVPGETFRKFIKDTYQQNLLCQNKMKLGRKLIKLKNVTCPLLNVVA
                     EHDDIVPLSSSTVVMDLVGSQEREEIRVKGGHHGISVGPGAIKLVWPKTVEWLANRE"
BASE COUNT          426 a          393 c          461 g          491 t
ORIGIN      
        1 atcgaaaccg tgttgagtaa ctggcctggc tcgggccccg gatcttcttc atcagagtat
       61 tccacagtgg tcccgggtgg agcgatgggt ttgagtttga cctgaatttc aacggtaacc
      121 ggcagtgttt cgccagagta atcaaggggt tcggtctgat ctggcggaat gttgccgaca
      181 agggcatcat tcatataccc ttttatggtt gaaacaatat agcccggata gttgcaagta
      241 actgtgcctg acgcagttgt ataacttccg gtttgcggga ttgtaaaggt aaacggggga
      301 ggatcggtat cttgaatggt gtgttgcgac atgaacgtac tccgagttgt atggtgtggg
      361 taaccagaat gctccgcagt actccgccat atatcctacc cgtgagcacg agtcaatcat
      421 tataccagtt tgtcgtcagc cgtcagtcgt caattgttcg ctaagtttat tcggttgaat
      481 tatttaactg gttgctaaga cgaggatttc attgcaaatt ggtgttactt cgaagtgaag
      541 acaggaggcg gaaaactttg gatgaaggcc atccgcctat gtgtgtggtt ccgtttcact
      601 cacggttggc aagccattcc actgttttag gccagacaag tttgatcgcg cctgggccaa
      661 ccgaaattcc gtggtgaccg cctttcactc gaatttcttc cctttcctga ctgccaacca
      721 gatccatcac caccgttgac gaactgagcg gaacaatatc atcgtgctca gccaccacgt
      781 tgagcagggg acaggtcaca tttttgagct tgatgagttt tcggccaagc ttcattttgt
      841 tttggcaaag caggttttgc tgataggtgt ctttgatgaa ctttcgaaat gtctcaccag
      901 gaacggcaac ggcgtcatta acccagtagt caaatgcctg aaacattttg acatactctt
      961 cattatgaag attttcggcc aggtggacat acttggtgac attcttggtg ggcttgagca
     1021 tcatgaaggt gcttttcaga atttcaaccg gggcattccc atggcggtca ataaattgat
     1081 caacattgaa atactctggc tgggcccagg tactcaacag cgaatcgtta tgaaaatcca
     1141 ccggtgtggc cagcaggatc agattcttga gaaattgttt atgcagggcg gcatacatca
     1201 gcgccatcgt tccgcccata cagtagccga tcattgagac ttgctcagcg ccagcgtggg
     1261 tcagcactgc acgtgtgata ttgaccagat atttattgat atggtcttcg agcgtcaggt
     1321 gggaatcgtg gtcgtctggc tctccccaat caatcatata cacacgaaat ccctgattgg
     1381 ccagatactc gacataactc ttccctggca tcaaatcaag gatatagtat ttattgatca
     1441 gtgagttaac aatcagaatc ggtaccgagt gcgtcttttt gacttttggc aggtaataga
     1501 gaacccggag cttgttttca cgatacacga ccttcgaggg ggttttggca acctcgggtg
     1561 gttgtgatat ctgcgagagc atttctgaga gtttgagtaa gtcaaacata gttttgggtt
     1621 ccgggttccg ggttccgggt ttgaaatttg tataggttga ggttttcgcc gcgtagcggt
     1681 ggttgaattg agacaggtcg tttacggcct gctgcgaaaa ggaccaaaat tcgaaaaccc
     1741 ggaacccgga acccggaacc cggaacccgg a
//
LOCUS       DAOQDO010001532         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70743_length_1770_cov_2.664723, whole genome shotgun sequence.
ACCESSION   DAOQDO010001532 DAOQDO010000000
VERSION     DAOQDO010001532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70743_length_1770_cov_2.664723"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="PKZ53_20485"
     CDS             <1..1713
                     /locus_tag="PKZ53_20485"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNJ42872.1"
                     /translation="VDFEGQTASLPKLLAALRRGERFVQLGDGSFGMLPEEWLAKYGL
                     LTGVGQAEGDTIKFRRSQAGLLDALLSTQPQIAVDEPFIKLRDQLHQFDKVAAQLPPP
                     GFIGELRPYQQEGLGWLPFLDQFGFGGCLADDMGLGKTVQVLAFLQAAKEHKEATPGR
                     PRPSLVVVPKSLLFNWTEEARRFTPQLQILTHVGTGRDQEFDSFQAFDIILTTYGTML
                     RDILKLKDIQFDFVILDESQAIKNASTETSKAARLLQGRRRLAMSGTPIENHLGEFWS
                     LFEFLNPGILGTASVFQQLVDDPNLRQIESRELLAKAVRPFLLRRTKQQVAKDLPTKT
                     EQTIYCEMEPEQRKHYLELRDYYRATLLGQIGTEGIQKNKLQILEGLLRLRQAACHPG
                     LIDSKQASKSSAKLDVLLPYIAEIVDEGHKALIFSQFTSFLSIVRKQLDKEKIPYLYL
                     DGKTQNRGELVEKFQTDPKSKLFLISLKAGGFGLNLTAAEYVFLLDPWWNPAVEAQAI
                     DRTHRIGQTEQVFAYRLITRDTVEEKVLKLQENKRNLADAILGENSATLRDLGREDLE
                     LLLS"
BASE COUNT          425 a          458 c          456 g          431 t
ORIGIN      
        1 gttgattttg aaggccagac agcttccctg cccaagctcc tggcagccct gcggcgcggt
       61 gaacggtttg tccaacttgg tgatggttcg tttgggatgc ttcctgaaga atggctggcc
      121 aaatacggcc tgttgaccgg cgttggtcag gctgagggag ataccatcaa atttcggcgg
      181 tcacaggccg ggctgctgga tgcgcttctt tctacccagc ctcaaattgc agtggatgaa
      241 ccatttatca agctccgtga ccagttgcac cagtttgata aagtggcggc tcaactcccg
      301 ccgcctggat ttatcgggga acttcgcccc tatcaacagg aagggctggg ctggctccct
      361 tttctggatc aatttgggtt tggtggctgt ctggccgatg atatgggact cggaaaaacg
      421 gttcaggtgc tcgcgtttct tcaggcagcc aaagagcata aagaagcgac cccaggtcgc
      481 ccacgtccat cactggttgt ggtaccaaaa tcgctcctgt ttaactggac agaagaagcc
      541 cgccggttta ccccgcagct tcaaatcctc acccacgtcg gcacgggtcg tgaccaggaa
      601 tttgattcat tccaggcgtt tgacatcatt ttgaccacct atggcaccat gctgcgtgat
      661 attctcaaac tcaaagacat ccagtttgac tttgtgattt tggatgaatc gcaggccatc
      721 aaaaacgcct caactgagac ctccaaagct gccaggttgc tgcaaggccg ccgacggctc
      781 gccatgagcg gaacaccgat tgaaaaccac cttggtgaat tttggagcct ctttgaattc
      841 ttaaatccag ggattttggg cacggcttcg gtctttcagc aactcgtgga tgaccccaat
      901 ttgcgccaga tcgaatcgcg tgaattgctg gccaaagctg tccggccatt tttgcttcgg
      961 cgcaccaaac aacaggttgc caaagatctg ccgaccaaaa ccgagcaaac catttattgt
     1021 gaaatggagc ccgaacagcg caagcactat cttgaactgc gcgattatta ccgggccacg
     1081 ttgcttggac aaatcggaac cgaaggcatt caaaaaaaca aactgcaaat ccttgaaggc
     1141 ttgctccggt tgcgccaggc cgcctgccat cctggactca ttgattccaa acaggcttcc
     1201 aaatcaagtg ccaaactgga tgtgttactg ccctacattg ccgaaattgt ggatgaaggc
     1261 cacaaagcct tgattttctc gcaatttacc agttttcttt cgattgttcg caaacagctt
     1321 gataaggaaa agattcccta tttgtacctg gacgggaaaa ctcagaaccg gggtgaactg
     1381 gtggaaaaat tccagactga tccaaaaagt aaattgtttc tgatcagtct gaaagccggc
     1441 ggatttggtc tcaacctgac agccgcggaa tatgtctttc tgcttgatcc gtggtggaat
     1501 ccggcggttg aagcccaggc aattgaccga acgcatcgca tcggacaaac tgaacaggtt
     1561 tttgcttatc ggctcatcac tcgcgatacc gtcgaagaaa aggtgctaaa actccaggaa
     1621 aacaaacgca atctggcaga tgctattttg ggtgaaaaca gtgccacgct acgcgatctg
     1681 ggtcgggagg atctggaact gttgctgtcc tgaagaattc cgggttccgg gttccgggtt
     1741 ccgggttccg ggttccgggt tccgggttcc
//
LOCUS       DAOQDO010001533         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_70922_length_1768_cov_1.575598, whole genome shotgun sequence.
ACCESSION   DAOQDO010001533 DAOQDO010000000
VERSION     DAOQDO010001533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70922_length_1768_cov_1.575598"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKZ53_20490"
     CDS             <1..390
                     /locus_tag="PKZ53_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42873.1"
                     /translation="KGSSGKQKLIYPHGGGISQVQVKKQIVLPSGEDWIRFDEQTGTE
                     QVTFLMSKKPFKAEVEASGGTGGQPVSGSIGGVANPQEEAEILAALNKSLPKDLEYDS
                     GNNGFTVTSSQNFTKPIKIELNLKHGR"
     gene            392..1453
                     /locus_tag="PKZ53_20495"
     CDS             392..1453
                     /locus_tag="PKZ53_20495"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ42874.1"
                     /translation="MKSGVLAVALLLSGCFGSVMAQEAKGPEKLRSAILATVRIITGS
                     LENGEFQGQSSGSGVIISPRGYVLTNFHVISDKGKPYPELWAGLVDPRQDYLPPNQAL
                     RLKLIGVDVELDLALLKLEPKASKPVSFPFLRLGDSGGLTYGSPLTIVGFPISVGPTT
                     TVTRSVVIGIDVDDGWIKVDGGLLPGISGGAVVNDQAELMGIPTKVQTNQQIPFFGDS
                     DVPLGMMVLSNVGYVRSAEKLAEFLSRAAVTEFPSTTPKLVRVIGKVIDANNRPVSGA
                     VIGILSPATTAPEVYVGRKDLMAYAKSARDGEFQVNRFLRPGKYIFKVVCQGYESVIE
                     ELEVTSQNHDFEIKLAPTD"
     gene            1502..>1768
                     /locus_tag="PKZ53_20500"
     CDS             1502..>1768
                     /locus_tag="PKZ53_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42875.1"
                     /translation="MSTRYTSLCFGMSLLIGLFILKPMVVPGAISPGTNKLSQDEFSP
                     DEEDSPALRRCAAYKSPEEIEAEEKEFSAKMISLSKSGAVPGGGQ"
BASE COUNT          442 a          394 c          498 g          434 t
ORIGIN      
        1 aagggcagtt cgggcaaaca aaaattgatt tacccacatg gcggtgggat ttcccaggtt
       61 caggtgaaga aacaaattgt gttgccttct ggcgaggact ggattcggtt tgatgaacaa
      121 accggcaccg aacaggtgac cttcttaatg tcaaagaaac cgttcaaggc cgaggtggaa
      181 gcatcaggag gaactggggg ccaaccggtg agtggttcga ttggtggcgt tgccaatccc
      241 caggaagaag ccgagatttt ggcagcgctc aacaaatcgt taccgaagga tctggaatat
      301 gattctggaa ataatggttt taccgttacc tcaagccaga attttaccaa accgattaaa
      361 atcgaattga acctcaaaca cgggaggtag catgaaatca ggtgtgttgg cggtggctct
      421 gttgttgagc gggtgtttcg gatcggtgat ggcccaggag gcaaagggcc cagagaaatt
      481 gcggtctgcg attctggcaa cggttcgaat tatcactggc agtcttgaga atggcgaatt
      541 tcaaggacaa agctccggct ccggtgtgat catttccccc agggggtatg ttttgacgaa
      601 ctttcacgtc attagcgaca agggaaaacc ctacccggaa ttgtgggccg ggctggttga
      661 tccgcgccag gattaccttc ctcccaacca ggccctgcgg ctcaagttga ttggagtaga
      721 cgtcgagttg gatttggcat tgctgaaatt agaacccaaa gcatcaaaac cagtgtcctt
      781 tccttttctc aggctgggtg attcgggagg gctgacgtat ggctccccgt taaccattgt
      841 ggggttcccg atttcggtgg ggccgacaac gacggtaacc cggtcggtgg tgatcggaat
      901 agatgtcgat gacgggtgga tcaaagttga tggcgggttg cttccgggaa tttcaggcgg
      961 ggctgttgtg aacgatcagg ccgaactgat gggcattccg accaaggtgc aaaccaacca
     1021 gcaaattccc ttttttggag atagtgacgt cccgcttgga atgatggttc tttcaaatgt
     1081 cgggtatgtt cggtcagccg agaaattagc cgaatttttg tcacgagcgg cagtcactga
     1141 atttccgtca accacaccga aactggttcg ggtcattgga aaggtgattg atgccaacaa
     1201 tcgcccggtt tccggagcgg tgattggtat tttgtcacct gccacgaccg cgccggaagt
     1261 gtatgttggc cgaaaagatt tgatggccta tgccaaaagt gcccgagatg gtgagtttca
     1321 ggtcaatcgt tttttacggc ccggaaaata cattttcaaa gttgtatgtc agggctacga
     1381 atcagtcatt gaggaattag aggtaacttc ccagaaccat gattttgaaa tcaagctggc
     1441 acctactgac taaccctggt gcggtttttc caaaactgta ctttatttgg agccaattgg
     1501 catgagtaca cgatacacat cactttgctt cggaatgagt ttgctcattg gcctgtttat
     1561 cctgaaaccg atggtggtcc caggtgccat atcaccaggg accaacaaac tgagccagga
     1621 tgaattttcg ccagacgaag aagactctcc agcgctccgc cggtgtgccg cctataagtc
     1681 gccggaagaa atcgaagcgg aagaaaaaga attttcagcc aaaatgattt cgctttcaaa
     1741 atcgggcgcc gtgcctggtg gcgggcag
//
LOCUS       DAOQDO010001534         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71081_length_1766_cov_1.718293, whole genome shotgun sequence.
ACCESSION   DAOQDO010001534 DAOQDO010000000
VERSION     DAOQDO010001534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71081_length_1766_cov_1.718293"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="pgl"
                     /locus_tag="PKZ53_20505"
     CDS             complement(<1..1051)
                     /gene="pgl"
                     /locus_tag="PKZ53_20505"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNJ42876.1"
                     /translation="MDRQISQSTKIKRTLWRSFSVILVLGVAMAALAYGPRLLKPTIG
                     RADFRTARVEAGPIEATINSTGVVVPDFEQVISSPVNGRILRVLKRPGDPVRRGDAIL
                     ELDVSEATLALEKLKQQIDLKQNQQAKARLDLQNTLNTLRSQWEIKNLDYEAAKASTG
                     CNRKLKELGLLSAEQLHEAIARSRSASELLWEHVEWWWGDERFVPSGSEQRNDVMAQR
                     SLFDRIQITPQKVHSMPASDDSFRTIQDAADSYTAQLTRQAPDGFDIVLLSLGPDDHI
                     ASLFPDSTSLSARQVKAIAITSAPKPPPERITLALEALAHTKNLWIMASGSEKAGAVK
                     AAISDQATDLPAVRLS"
     gene            complement(1425..>1766)
                     /locus_tag="PKZ53_20510"
     CDS             complement(1425..>1766)
                     /locus_tag="PKZ53_20510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ42877.1"
                     /translation="PGGRAAILEFSRPILPGFCEAFQWYFNHILPRIGGLISGSSMAY
                     TYLPTSVKSFPDQKQLADLMRKVGFTDVKYHNLSGGIAALHLGNKGKAEGGRRRAEEN
                     GLRAEDSQARG"
BASE COUNT          382 a          469 c          489 g          426 t
ORIGIN      
        1 ggctcaggcg tacagctggg agatctgtcg cttgatccga tattgcagcc tttaccgcac
       61 ctgccttctc acttcctgag gccatgatcc acaggttctt cgtatgggcc agcgcctcaa
      121 gagcgagggt aattcgctca gggggtggct ttggagcaga agtaattgcg atcgctttga
      181 cttgtcttgc cgacaaactt gtcgaatcgg ggaagagtga ggcaatgtgg tcatctggac
      241 ccaagctcaa caacacgatg tcgaatccat cgggggcttg ccgggtcagt tgtgcagtgt
      301 aggagtcagc cgcgtcctgg atggttctga atgagtcatc cgatgctggc atcgaatgaa
      361 ccttctgcgg cgtgatctgg attcggtcaa agagagatct ttgcgccatg acgtcattgc
      421 gctgttcaga tccgctcggc acgaatcttt cgtcgcccca ccaccattca acgtgttccc
      481 acaggagttc ggatgcggac cgactccttg cgatggcttc gtgaagctgt tcggcagaga
      541 gcaaccccag ttctttcaac ttccggttgc aaccggtcga agctttggcc gcttcataat
      601 cgagattttt gatttcccac tggctgcgga gcgtgttgag ggtgttttgc aggtccagtc
      661 tggctttggc ctgctggttt tgtttgagat caatttgttg tttgagtttt tcaagcgcca
      721 gcgtagcctc gctgacatcc agttccagaa tggcatcacc acgccggacc ggatcaccag
      781 ggcgctttaa cacccgcaga atccgaccat tcaccggact ggaaatgacc tgttcaaaat
      841 cgggcaccac caccccggtg gaattgatcg tcgcttcaat tggaccggct tcgacccgtg
      901 ccgtgcgaaa atcggcccgt ccaatggtgg gcttgagcag ccgtggcccg tatgccaggg
      961 ctgccatcgc cacaccaagg acgagaatta ccgaaaaact ccgccagagc gttcttttga
     1021 ttttggttga ttgactgatt tgacgatcca ttgatttggt ctcctcacgt ttgcaccggg
     1081 ttgagtgcaa gcaagagacc acaactggac aaaaggcgag tcagggctca gggctcaggg
     1141 ctcagggctc agggaaatcc aatctttcca acagtttaat ctctcgaaat aatttggatc
     1201 actggcttta aatgagccct gttttcgaac gtaacgattg atttcaggcg ggatacaagg
     1261 catttgacag ggcgaaggcg tacgcaaatg tacgggggtg tacggaaacg gacggtctgg
     1321 ggctgaagaa aacgggctga gggctgaaga aaacgggctg ggggctcagg gtattgaacc
     1381 cattttcttc agccctcagc cctcagccct cagccctggt attttcagcc ccgagcttgc
     1441 gagtcttcag ccctcagccc gttttcttcc gcccgacgtc ttccgccctc tgcctttcct
     1501 ttgtttccca aatgcagcgc ggcaatccca cccgaaaggt tatggtattt gacgtcggta
     1561 aacccgactt tgcgcatcag gtcagccagt tgtttctggt caggaaagct tttcacggaa
     1621 gtcggcagat aggtataggc catgctcgaa cccgaaatca ggccgccaat gcgaggcaaa
     1681 atgtggttaa agtaccattg gaaggcttca caaaaccctg gcagaattgg acgggagaac
     1741 tccagaatgg cggcccggcc tccagg
//
LOCUS       DAOQDO010001535         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71100_length_1765_cov_3.643860, whole genome shotgun sequence.
ACCESSION   DAOQDO010001535 DAOQDO010000000
VERSION     DAOQDO010001535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71100_length_1765_cov_3.643860"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..>1765)
                     /locus_tag="PKZ53_20515"
     CDS             complement(235..>1765)
                     /locus_tag="PKZ53_20515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ42878.1"
                     /translation="MTTPVTPKLAAGIVLLRQSSVTGWEFFWARRSNKMPFMSGFHAF
                     PGGRLDKSDAELTIEQASSPEQAALCVCAIREAFEEAAILISPDAHKLTVEERLTART
                     ALMQNQLTFAEFVNRHSLRLDARLLVPAGRWVTPPYAARRFDTWFYTAEIPEGQEPEL
                     WTDELEHGTWATPLEAERRWADLQVLLAPPTLHTIRGIGRWIETGAGEKRDWGHLTRL
                     LTNIPEANGKPIEWIETRPGIFVFPVRTPTLPPATHTNCYLIGDQELIVIDPASPYAD
                     EQARLDAHLADLRERTGARVREIWLTHHHPDHVSGVEHARTTWGVPVAAHPKTAELLN
                     GKVTVDRFLNDGDVLELSGNPGWRLRAIFTPGHAPGHLCFLEENSRAIVTGDMVVGLG
                     SVLIAPPDGNMQLYLDSLEKLSKLSLTMILGGHGQAMGSPQKHIASYIKHRLDREAQI
                     LAAMTTGAETIDDIVKIAYATTPVEMHPYAALSVKAHLEKLLNEQRITPVGEARWQVA
                     G"
BASE COUNT          417 a          470 c          450 g          428 t
ORIGIN      
        1 ttgtcaaaag tttcctgaga tattgaattt tggaagtgtt tggttttttt cgtgtatttc
       61 gtgtatttcg tggttcaaaa tgtctggaaa ttttcggtaa ggtacttatt gatttccaag
      121 caccaagaac caggcaccaa gaaccaggca ccaaggacac acttcattct caattcttca
      181 ttctcaattc ttcattcttc attcttcatt ctcaattctt cattcttcat tccatcaccc
      241 ggccacctgc caccgggctt cgccaactgg agtaatccgt tgttcattca aaagtttttc
      301 cagatgggct ttgactgaaa gtgcggcgta cgggtgcatt tccaccgggg ttgtggcata
      361 ggcaattttg acaatgtcat caatcgtttc ggccccagtc gtcatggctg caagaatctg
      421 cgcttcgcgg tccaggcggt gtttgatata gctggcaatg tgcttttgcg ggctgcccat
      481 tgcctgacca tggccaccca aaatcatggt aagtgacagt tttgaaagct tttccagtga
      541 atccagatag agttgcatgt tgccatccgg aggggcaatc aggaccgaac ccagtcccac
      601 caccatatcg ccagtcacaa ttgcgcggct gttttcttcc aaaaaacata aatgccctgg
      661 agcgtgaccc ggtgtgaaaa tggcacgaag tcgccagccg ggattacctg aaagttccag
      721 cacgtctcca tcattcaaaa accggtcaac cgtaactttg ccgttgagca attcagccgt
      781 tttgggatgt gcagccaccg ggacacccca cgtcgtgcgg gcgtgttcga cccctgaaac
      841 gtgatcggga tgatgatggg tgagccagat ttcacgcacc cgagcaccgg tgcgttcccg
      901 taaatcagcc agatgggcat ccagtcgcgc ctgctcatct gcgtagggcg aagccggatc
      961 aatcacgata agctcttgat ctccaattaa atagcagttg gtatgagtag caggtggcag
     1021 cgtcggagtt cgaactggaa acacaaaaat gccaggtcgg gtttcaatcc actcaattgg
     1081 tttgccattg gcttcaggga tattggtcaa gagacgcgtc agatgtcccc aatcacgttt
     1141 ttctcccgca ccagtttcaa tccagcgacc aatgccccgg atggtatgca gcgtcggcgg
     1201 cgccagcaaa acctgcaaat ccgcccagcg gcgttcggcc tccagcggcg tggcccaggt
     1261 gccgtgttcg agttcatcgg tccagagttc gggttcctgc ccttctggaa tttcagccgt
     1321 gtaaaaccag gtgtcaaagc gccgggccgc atagggcggc gtcacccagc gaccagccgg
     1381 aaccagcagg cgcgcatcaa gccgcaggct atgccggttg acaaattcag cgaacgtcaa
     1441 ttgattctgc atcaaagcag tgcgggcggt aagtctttct tcgaccgtca gtttgtgagc
     1501 atcaggtgaa atcaagatgg cggcttcttc aaaggcttcg cgaatcgcac agacgcacag
     1561 agctgcctgt tcaggcgatg atgcctgctc aatcgtcagt tccgcatccg atttatctaa
     1621 ccggccaccg ggaaacgcat gaaaaccgct cataaacggc attttattgg agcgccgggc
     1681 ccagaaaaat tcccagccag tcacggaaga ctggcgaagt aagacaattc cggcagcaag
     1741 tttgggagtg acaggagtag tcata
//
LOCUS       DAOQDO010001536         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71134_length_1765_cov_2.579532, whole genome shotgun sequence.
ACCESSION   DAOQDO010001536 DAOQDO010000000
VERSION     DAOQDO010001536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71134_length_1765_cov_2.579532"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKZ53_20520"
     CDS             complement(<1..468)
                     /locus_tag="PKZ53_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HNJ42879.1"
                     /translation="MAIQRNDWSLQRKGIMDQERHAERVREAVKKNLGSIVSNESIIL
                     SDGKKSVKIPLRSLDEYKFRFDYRKQKHVAQGDGHSQVGDVVAREGKPGKGQGKGQAG
                     NQEGQEFYEAEVNIDEIAALIFEDLALPFLEEKAKQAVPSRSTRFTEIRRTGIM"
     gene            complement(496..1542)
                     /locus_tag="PKZ53_20525"
     CDS             complement(496..1542)
                     /locus_tag="PKZ53_20525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ42880.1"
                     /translation="MKRQNLITALVGLGLAGGWVWRYQRRPRDIRWIDYAGEIHHPMA
                     SNFAVVDEVRLHFQEKGLHNPDTLMLLHGFGSSNFTWKDCLNPLAEDGYRVIAPDLKG
                     FGFSEKPADGRYHVQDQAQLMIGLLDQLEIEQATLCGTSYGAGVALACALMWPNRVKR
                     LILMDAAYNDAPLHQPLPTAHLALARTYGIAEATIPLLFGSKVFVRNSLKTMFSDPSL
                     VDAERTQAYFRPVQSINCQQAFMTGARQWDLNWLELELSAITVPTLILWGEEDRIIPL
                     HLGGQLHLAIPQSEFYVLPKCGHVPQEECPIETVELMIDFLRRTHMRARPEEFPEDGP
                     GSAPPSVEYGDLDR"
BASE COUNT          428 a          451 c          426 g          460 t
ORIGIN      
        1 cataattcca gtccgtcgga tttcagtaaa acgggtcgag cgagatggca cggcttgttt
       61 ggctttttct tccagaaacg gtaaggcaag atcttcaaaa atcagggcgg cgatttcgtc
      121 aatattgact tcagcctcat aaaattcctg tccttcctga tttccagcct gtccttttcc
      181 ctgtcctttt cctggtttcc cctcccgagc cacgacgtct cccacctgcg aatgaccatc
      241 accctgggcg acatgttttt gtttgcggta atcaaaccga aatttgtatt cgtccagaga
      301 tcgaagtgga attttaactg actttttccc atccgacagg atgatggatt catttgacac
      361 aattgagccc aggttttttt tgactgcctc acggacacgc tccgcgtggc gttcctggtc
      421 catgatccct ttccgctgta gactccagtc gttgcgttgt attgccataa agagaacctc
      481 aactttcaga ggtagttagc ggtctaaatc accatattcg acagagggtg gtgcggaacc
      541 cggaccgtct tcgggaaatt cttcaggacg tgctcgcatg tgtgtccgcc gtaaaaaatc
      601 gatcatcagt tcgaccgttt caatcgggca ttcttcctgt ggaacgtgac cacattttgg
      661 aagcacataa aactcagact gtggaattgc caggtgcagt tgtccaccca gatgaagtgg
      721 gatgatccgg tcttcttcac cccaaagaat cagagttgga acggtgatcg cacttaattc
      781 cagctcaagc cagttcagat cccattgccg ggcgccagtc ataaaggctt gctgacaatt
      841 aattgattga acaggtcgaa aataggcttg cgttcgctcg gcatcaacca gtgaggggtc
      901 agaaaacatg gttttcaggc tattgcgcac aaaaaccttg gaaccgaaaa gaagcgggat
      961 cgtggcttcg gcaattccat aggtccgcgc caaagccagg tgagccgttg gaagcggctg
     1021 gtgaagcggg gcgtcattat aggccgcatc cattaaaatc agccgtttga cccgattggg
     1081 ccacattaac gcacaggcca gtgcgacgcc ggctccatac gaagtcccgc acagggtggc
     1141 ttgctcaatt tcgagttgat cgagcaatcc aatcatcagt tgtgcctgat cctgaacatg
     1201 gtagcgccca tcggctggtt tttctgaaaa cccaaagcct ttcaaatctg gggcaatcac
     1261 tcgatagccg tcttcagcca gtggattcag gcagtctttc caggtgaaat tcgatgaccc
     1321 gaacccatgc aggagcatca gggtgtctgg attatgcaat cctttttcct gaaaatggag
     1381 tctgacttca tccaccacag caaaattgct ggccatcggg tgatggattt caccggcata
     1441 atcaatccat cgaatatcgc gtgggcggcg ttgataccgc cacacccacc cacctgccag
     1501 tccaagtccc accaatgcgg taatgaggtt ctgtcgtttc acaataagag tctctccgat
     1561 ccaaaagtaa caagaagcag ttattttatc acgatttcac cttcagccgg gcctgaaaat
     1621 cagcacgtag cgccgaccga ggaaaagctg ggtttcaact tgtgtggtat cagtcaatta
     1681 ccggcaagga tgattgttcg tggcttctga tttgagaagc ttgcttttta taagtcagtg
     1741 ttcacaggtg ctgttttgag cagct
//
LOCUS       DAOQDO010001537         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71185_length_1764_cov_3.681685, whole genome shotgun sequence.
ACCESSION   DAOQDO010001537 DAOQDO010000000
VERSION     DAOQDO010001537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71185_length_1764_cov_3.681685"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PKZ53_20530"
     CDS             complement(<1..1648)
                     /locus_tag="PKZ53_20530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ42881.1"
                     /translation="MDNLEIEFLKRLLQTFTLEADERLHVIGQHLVELETASDLSTQM
                     TATEATFREFHSLKGAARSVNFTAIEKLCQECESVLELVKKKKVPLDKRVFDGLHDAL
                     EQVTRLLPQGTSAPLTSQDMALISRLRALQDVAPISEPVPIPAVETQPEEPAGIPVSF
                     SSLFQLVQPEEPEPVSAPPFTEVPLPPVVELIAPPAVTVPPPPPVTLPGAARAPESPV
                     IATGSRAETVRLPKYRLEQVLIQAEEMVLVKQMLNHQLRTLKRLTGNLGTTIRTLNQS
                     GAPFPSSSTASDELEQLTALQTELEDFATQTAADSSLVHRLTDDLLNGLKHLFLVPLS
                     LVFESVPKIVRDLARSLGKDVDLQLQVGEIEIDRRISEEMRDPMIHLLRNALDHGLEL
                     PELRQQRGKPSRGSLRVIATQIEGNKVVIEVIDDGEGIDPGKVRVACLKAGIRTEQEI
                     AQLSDQEIIQFIFQSGVSTSPIITDLSGRGLGMAIVRDKIERLGGDIQVDSVPGQGTT
                     LRIILPLTVATVRGIVVNEAGRTYILPTLTVENVVRAAQSE"
BASE COUNT          404 a          452 c          489 g          419 t
ORIGIN      
        1 tttcagattg ggcggcccga accacgtttt caaccgtcaa ggttggtaaa atgtaggttc
       61 gcccggcctc gttgaccaca atgccccgga cagtggccac ggtcaatggc aggataatcc
      121 ggagtgtcgt tccctggccg ggtacggagt caacctgaat atcaccgcca agccgctcaa
      181 ttttatcacg gacaatggcc attccaagac cacgccctga aaggtcggta atgatcgggc
      241 tggttgatac gccagattga aaaatgaact ggatgatttc ttgatcagag agctgggcaa
      301 tttcttgctc cgtgcggatg cccgctttga gacaggccac gcgaactttg cctggatcaa
      361 tcccttcacc atcgtcaatg acttcaatca cgactttgtt cccttcaatc tgggtcgcaa
      421 tcacccgcag actcccccgt gagggttttc cccgttgctg gcgtaattca ggaagttcga
      481 gaccgtgatc aagcgcattg cgcaggagat ggatcatcgg gtcacgcatc tcttctgaga
      541 tccggcggtc aatttcgatc tccccaacct ggagttgcag atctacgtct ttcccaagcg
      601 accgggccag gtcgcgaacg attttgggta ccgattcaaa caccagcgac agcggcacca
      661 aaaacaaatg ctttaatcca ttgagcaagt catctgtcag ccggtggacc agcgatgaat
      721 cggcggcagt ctgggtggca aaatcctcca actctgtttg gagcgctgtc agttgctcta
      781 attcgtcaga agcggtgctg ctggatggga atggcgcacc tgattgattg agggtgcgga
      841 tggtggtgcc cagattcccg gtcagtcgtt tcagggttcg caactggtga ttgagcattt
      901 gtttgaccag aaccatttct tcagcctgga tcaaaacctg ctctaaccgg tatttaggta
      961 agcgaaccgt ttccgccctg gacccagtcg caataactgg tgattcaggc gcgcgcgcag
     1021 cacccggtag tgtgactggt ggtggcggcg ggaccgtcac ggcgggcggt gcgattagct
     1081 ccaccactgg cgggaggggc acctcggtga agggaggcgc cgataccggc tctggttcct
     1141 caggttgaac cagttgaaac aaacttgaaa atgaaactgg aatcccggcg ggttcctctg
     1201 gttgagtctc aactgcagga atggggaccg gctccgagat tggagcaacg tcctggagcg
     1261 ctcgcaaacg actgatcaag gccatgtcct ggctggtcaa tggcgctgat gttccctggg
     1321 gtaaaagcct ggtaacctgt tcaagcgcgt cgtgtaaccc atcaaacacc cttttatcaa
     1381 gcggtacttt ctttttcttt accagctcaa gaacactttc acactcctgg cagagttttt
     1441 caatagcagt gaaattgacg gaccgggcag cacctttcag gctatgaaat tcacgaaatg
     1501 ttgcttcggt ggctgtcatt tgggttgaca gatcagatgc ggtttcaagt tcaaccagat
     1561 gttggccaat gacatgcagt cgctcgtcgg cttcgagggt aaaggtctgc aacaggcgtt
     1621 tgaggaattc aatttcgaga ttatccatat caagaatctg gagctgaaga cgtcaggctg
     1681 aaaacttcgg gctgaaaaat ccttgggctc agggcctggg gctgaagatt cgcaagctca
     1741 ggactgaaga aaatgggttc catc
//
LOCUS       DAOQDO010001538         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71275_length_1763_cov_3.475995, whole genome shotgun sequence.
ACCESSION   DAOQDO010001538 DAOQDO010000000
VERSION     DAOQDO010001538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71275_length_1763_cov_3.475995"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1672)
                     /locus_tag="PKZ53_20535"
     CDS             complement(119..1672)
                     /locus_tag="PKZ53_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ42882.1"
                     /translation="MAKAKAETSKTAKSTTAKTSQTGPAEYKNFIGGQWVPAANGETF
                     ENRNPADTRDLVGVFPNSTVEDVNRAVDAARDAYKSWRLVPAPKRAEMIFRVADILVR
                     RKEEISRDMTREMGKILKETRGDTQEAIDMAYFVAGEGRRLYGHTSPSELRNKFAMSV
                     RDSVGVCGMITPWNFPIAIPSWKLLPALLCGNTAVIKPAEDTPLSVVNFMKAFEEVGL
                     PPGVVNMVTGYGATAGNPLVNHPTIRVISFTGSTQTGRVISTQAAANGKRCSLEMGGK
                     NAIIVMDDANLDLAVDGALWGAFGTSGQRCTASSRLIVHKKVVKKFSAALVERVKALR
                     VGPGLDERSEMGPVINQKQMEKVLGYIETGKTKDKAKLLCGGERLTEGDLQYGYFIAP
                     TVFGDVDAKATIAQEEIFGPVTTIIPCDSFDHAIEIANGVEYGLSSSIYTGDVNRAFS
                     ALRDLETGICYVNAPTIGAEVHLPFGGTKGTGNGHREGGLEAFDVYTEWKSIYVDFSG
                     VLQKAQIED"
BASE COUNT          417 a          492 c          424 g          430 t
ORIGIN      
        1 gggcttgggg ctgaagacat caggctcagg ggattgaacc cgttttcttt agccccgagc
       61 ttgcgagtct tcagccccaa gcccaaagtc ttcagcccaa agtcttcagc cccaaatttt
      121 agtcttcaat ctgggctttt tgcagcacac cgctgaaatc aacataaatg gacttccatt
      181 cggtgtagac atcaaatgct tccagtccgc cttcacggtg accgttcccc gtgcctttgg
      241 taccgccaaa tggcaggtgg acttcagccc caatcgtcgg agcatttaca tagcagatgc
      301 ctgtttccag atcacgcagg gctgaaaaag cacggttgac atcgccagtg tagattgaag
      361 atgacaatcc gtactcaacc ccattggcaa tctcaatcgc atggtcaaag ctatcgcatg
      421 ggataatggt cgtgactggc ccaaagattt cttcctgggc aattgtggct ttggcatcaa
      481 catcgccaaa aaccgttgga gcgatgaaat atccatactg caaatcgcct tcggtcaggc
      541 gttcaccacc gcacaagagt ttggctttgt ctttggtttt gccggtttcg atatacccaa
      601 gcaccttctc catttgcttc tggttgatga ccggtcccat ttcactccgc tcatcaagcc
      661 ctggaccaac ccgcaacgct ttcacgcgtt ccaccagcgc cgccgaaaat ttcttgacga
      721 ctttcttgtg gacaatcaag cgtgatgaag ccgtgcagcg ttgacctgaa gttccaaagg
      781 caccccagag ggcgccatca accgccagat ccagattggc atcatccatc acgataatgg
      841 cattttttcc gcccatttcg aggctgcagc gtttgccatt ggcagcggct tgtgtcgaga
      901 tcacccgacc agtttgggtt gagccagtaa aggaaatcac ccgaatcgtc ggatgattga
      961 ccagcggatt gcccgccgtg gccccatacc cggtcaccat gttgacgact cctggcggga
     1021 gaccgacttc ttcaaacgct ttcataaaat tgacgaccga aagcggcgtg tcttccgccg
     1081 gtttgatcac cgccgtgttg ccacacagca gcgcaggcag gagtttccac gacggaatcg
     1141 caatcgggaa attccagggt gtaatcatgc cgcagacccc aaccgaatca cgcaccgaca
     1201 tggcaaattt gttgcgcaac tctgaaggag aagtgtgccc gtacagccga cggccttcgc
     1261 cagccacaaa gtacgccata tcaatggctt cctgggtatc gccacgggtt tctttcagaa
     1321 tcttgcccat ttcgcgggtc atatcgcggg aaatttcttc cttgcgacga accagaatat
     1381 cggccacccg aaagatcatt tcggcacgct tgggggcagg aaccaaccgc catgatttgt
     1441 aggcgtcacg agcggcatca accgcacggt tgacatcttc aacggtcgaa tttgggaaca
     1501 cacccaccag atcacgggta tccgccgggt tgcgattctc aaaagtttca ccattggcgg
     1561 ctggtaccca ttggccacca ataaagttct tgtattccgc gggtccggtt tgcgaagtct
     1621 ttgcagttgt agattttgca gttttgctgg tttcagcttt ggctttcgcc atggcactcc
     1681 tcctgagtat gaaaacttat gaagatttcg agatgaaaat taagaaaaat agagaccggc
     1741 cactatagtg aatttttcta aat
//
LOCUS       DAOQDO010001539         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71295_length_1763_cov_2.502342, whole genome shotgun sequence.
ACCESSION   DAOQDO010001539 DAOQDO010000000
VERSION     DAOQDO010001539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71295_length_1763_cov_2.502342"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PKZ53_20540"
     CDS             complement(<1..1599)
                     /locus_tag="PKZ53_20540"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ42883.1"
                     /translation="MSSRPSSFVSQTENLSLRPVPHFIPRALSGWFKKMLALDQIFDH
                     YQHILTTNPEVSAKEFFDQALSHFKIQYQATSAEPVVFPSGPLVIVANHPFGVIEGMI
                     LCSQVFKQRPDARLMANSLLSTFTPIRSHCIFVDPFGTVDSPRHNLAPLRQAIQWVRD
                     GGALIVFPAGEVSHLTLDQRAVSDPPWSPTIARLIRKSEAAVVPVFFEGRNSDLFQVA
                     GLTHPRLRTLLLPRECLKKQKTEVSFTVGTSIGFDHLTRFETDSQLIEYLRFRTYHLK
                     HQNQPASVIRLNQRSWFQRQPRPVSSQCFTRDQLALEISRLDASQKLAESGELAVYAA
                     SFAQIPSVMQEIGRLRELTFRLTGEGTGLALDLDQFDRHYTHLFIWNQTASEVVGAYR
                     LARVDQILESNGMKGLYTSTLFRFSKTLFQHFPQALELGRSFVRPEYQKSFSPLLLLW
                     KGIGAFVCQHPQYKHLLGAVSMSRAYSDVSRQMLVRYLTHHESLPGLFPLVQPRHPFQ
                     PGDLPASFPAWAQSFDSSLEHVSDF"
BASE COUNT          484 a          402 c          498 g          379 t
ORIGIN      
        1 aaaatctgaa acgtgctcaa gcgacgagtc aaatgattga gcccaggcag ggaagctggc
       61 tggtaagtca cctggttgga agggatgtcg cggctgtacc agcggaaata accctgggag
      121 gctttcatga tgtgtcaggt accgaaccag catctggcgc gagacatctg aatatgcgcg
      181 gctcatgctc acagcgccca gcaggtgttt gtattgtgga tgctgacaaa caaacgcccc
      241 aatacctttc cagagcaaga gcaacgggct gaatgatttc tggtactctg ggcggacaaa
      301 tgaccggcca agctccagtg cttgcgggaa atgttgaaac aatgtctttg aaaatcgaaa
      361 cagcgtcgag gtatagagtc ccttcattcc gttggattcc aaaatctgat caaccctggc
      421 caggcggtat gcaccaacga cttcagaggc ggtttgattc cagataaata aatgggtgta
      481 atgtcgatca aactggtcaa ggtcaagcgc cagaccggtg ccttcgcccg tcagccggaa
      541 ggtgagttcg cgcaatcggc caatttcttg catcacgctg ggaatttggg cgaaagaggc
      601 tgcataaacg gcaagctcgc cagattcggc gagcttttga gatgcgtcaa gtctggaaat
      661 ttccagagca agctggtcac gcgtaaaaca ctggctggaa actggcctgg gttggcgctg
      721 aaaccacgac cgctgattca accggatgac cgaggctggc tggttctggt gtttcaggtg
      781 ataggtccga aaccgaagat actcaatcag ttgcgaatca gtttcaaaac gggtgaggtg
      841 gtcaaatcca attgacgtac cgaccgtgaa cgaaacttcc gttttttgtt tcttgaggca
      901 ttcacgcgga agcagcaacg ttcggagccg cggatgggtc agtccagcca cttgaaacag
      961 gtctgagttg cgaccttcaa aaaagactgg gacgacggca gcttctgatt tgcgaatcaa
     1021 tcgggcaatg gttggactcc agggcggatc tgaaacggcc cgttgatcaa gcgtgaggtg
     1081 cgagacttca cccgccggaa acacaattaa cgcgcctcca tctcggaccc attgaattgc
     1141 ctgccggagc ggtgccaggt tgtgacgcgg agagtctaca gttccaaatg gatcaacaaa
     1201 aatgcagtgt gaccgaatcg gggtaaacgt actgagcaac gaatttgcca tgagccgggc
     1261 atctggtcgc tgtttaaaaa cctgactgca caaaatcatg ccttcaatca caccaaaagg
     1321 atgattggca acaatcacca gcgggcccga aggaaaaaca accggttcgg ctgaggtagc
     1381 ctgatactga attttgaaat gtgacagggc ctgatcaaaa aattctttgg cagagacttc
     1441 aggattggtg gtcaaaatat gctggtagtg gtcaaaaatt tgatccagcg ccagcatttt
     1501 cttaaaccag ccactcaggg cacggggaat gaaatgggga accgggcgca gggaaaggtt
     1561 ttcggtctgg gaaacaaagc ttgatgggcg actgctcaca ggcaagatcc tccggcttgg
     1621 gcattcaact ttgaaaccaa tgccgagtgt gacctggaag cgttacaccc ctgaggctga
     1681 acctttacgt ttgtgtgagg ttggtgttac ggagaatgaa gaatgaagaa tgaagaatga
     1741 agaaaaagac agaatgaaga atg
//
LOCUS       DAOQDO010001540         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71422_length_1761_cov_3.378664, whole genome shotgun sequence.
ACCESSION   DAOQDO010001540 DAOQDO010000000
VERSION     DAOQDO010001540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71422_length_1761_cov_3.378664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="PKZ53_20545"
     CDS             <1..1684
                     /locus_tag="PKZ53_20545"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNJ42884.1"
                     /translation="TAMTGHGSLPPLWALGFQQSRWSYFPEARVREIADNFRRRKIPA
                     DTIYLDIDYMDGFRVFTWDKKRFPDPKKLISDLRQDGFRTVVIIDPGIKVDEKYGVYQ
                     SGQAGNHFVKTADGKEFQATVWPGTCAFPDFTDPKARAWFGSQYAGNLDDGISGFWND
                     MNEPATFIPWDLKEPNTLNHPMKTFPVTNRHAGDGHPGDHARYHNVYGMQMARSTFEG
                     LRKLRPNERPFVLTRAGYAGIQRYSAIWTGDNVASWDHLALSIPMLTNLSISGVSLVG
                     ADVGGFSGNPSAELNTRWMQAAALTPMYRSHTEAGSNDQEPWAFGPEFEKINRQAIEL
                     RYQLLPYLYSLFHTHEQTGQPVMRPLWYQFPQDRNTYLIDDQYLIGQDLLVAPVVKQG
                     ATKRRVYFPAGTDWIDWWTGARLAGGNWADVDAPIDRLPLYVRAGAVVPTQPVVQNTG
                     EMARQPLFLTIAAGNNQQGEFFEDTGEGYGYRQGEFRLVKTVGTATELKFQSSGKFAG
                     RTIQKIELIGLEQPPKAVLVNGQETSAVAFDAARKRLVLTVSALPETVTLRN"
BASE COUNT          403 a          465 c          494 g          399 t
ORIGIN      
        1 taccgcgatg actggtcatg gctcattgcc gccactctgg gcactcgggt ttcaacaatc
       61 acgctggtca tattttcctg aagcccgcgt ccgtgaaatt gccgacaatt ttcgccgtcg
      121 aaaaattcca gccgatacga tttacctgga tattgattac atggacggct ttcgggtgtt
      181 tacctgggat aaaaaacggt ttcccgaccc gaagaaactg atttccgacc tgcgtcagga
      241 tggatttcgg accgtggtca ttattgatcc aggcatcaag gttgatgaga aatatggggt
      301 gtaccagagt ggtcaggcgg gaaatcattt tgtgaaaacg gctgatggca aagagtttca
      361 ggcgacggtc tggcccggca cctgtgcctt tccagatttt accgacccca aagcacgcgc
      421 ctggtttggg tcgcaatatg ccggaaacct ggacgacgga atcagtggct tttggaatga
      481 tatgaacgag ccggcaacct tcattccgtg ggatctcaag gagccaaaca cgctcaatca
      541 tccgatgaaa acctttcccg tgaccaatcg ccacgccgga gacggtcatc ctggtgacca
      601 tgcccgctac cacaacgtct atggcatgca aatggcccgg tctacgtttg aaggattgcg
      661 caaattgaga cccaacgaac gaccatttgt gctgacccgc gccgggtatg ccggcatcca
      721 gcgttattcg gccatctgga ccggcgataa tgtggccagt tgggatcatc tggcgctgtc
      781 aattccaatg ctgaccaacc tgagcatttc cggagtgtca ctggtcggcg ccgatgtggg
      841 cggtttttcc gggaatccat cagccgaact caacacccgc tggatgcagg cggctgcctt
      901 gacgccaatg tatcgctcac acaccgaagc tggttccaac gatcaggaac cctgggcctt
      961 tggaccggaa tttgaaaaaa tcaaccggca ggcaatcgaa ctccgctatc aactgttgcc
     1021 ctatctctac tctctgtttc acacccacga acaaaccggc cagccggtga tgcgtccgct
     1081 ctggtatcaa tttccacaag atcggaacac ctatctcatt gatgatcagt atcttatagg
     1141 ccaggatctg cttgtggcac cagttgtcaa acagggtgcg accaaacgcc gggtctactt
     1201 tccggctgga acggactgga ttgactggtg gactggcgcc cgcctggccg gggggaactg
     1261 ggcagacgtt gatgctccga ttgaccggct gccgctctat gttcgggcag gcgcagtggt
     1321 gccaacccaa ccggttgtcc aaaatacagg cgaaatggcc agacagccgt tgtttttgac
     1381 aattgcggct ggaaacaatc agcaaggtga attttttgaa gataccgggg aaggatacgg
     1441 gtatcggcag ggggaatttc ggctggtgaa aaccgtcgga accgcgacag aactcaaatt
     1501 ccaaagcagt ggtaaatttg cgggccggac aattcaaaag attgaactga ttggacttga
     1561 acagccgccg aaggcagtgt tggtgaatgg acaagaaacc tcagccgttg catttgatgc
     1621 ggcccgaaaa cgactggtac tgacggtttc agccttgccc gaaacggtga ccttgcggaa
     1681 ctagggttcc gcgcttccgg ggttccgggg ttccgggttc cgggttccgg gttccgggtt
     1741 ccgggttccg ggttccgggt t
//
LOCUS       DAOQDO010001541         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71436_length_1761_cov_2.871043, whole genome shotgun sequence.
ACCESSION   DAOQDO010001541 DAOQDO010000000
VERSION     DAOQDO010001541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71436_length_1761_cov_2.871043"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="PKZ53_20550"
     CDS             complement(<1..1597)
                     /locus_tag="PKZ53_20550"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ42885.1"
                     /translation="MRFDHLKKHPLIFWFSISLIFLTCSIQFQPAPFTVSAQSSGIRI
                     REINPVVNEQNQITLTAIDASNQPVTNVVWESGSPEIASVDAQSGRVSGVRQGFATIT
                     ARRGEASFSTFVVVTRVHSGRGAKVPGDTKTDAEGKIYLSDPVGSVILKKESFSLPAD
                     IFAGQRGATGNTNGNRQEAKFNAPTAVAVNNSLRGGVFIADTANHSVRKIDFSDQVTT
                     VLGSGAPGVMNADVTPFAGAVFRGLRGLAIDAGGNLFIADTENHAVYYADFARQEIRL
                     LAGSPGTSGKSDGTGRAARFFRPKGGAISPDGRLLAIADEGNNVVRLLDRTGTVRTLS
                     RKAGQRFAFAWPMPKQAGTEIRFDSPQAVSFDELGNLYVVDRTSAKVITQALTDQADV
                     FDLAQPGSFSQAVSVVVRGTESFVLDANAMTDDEAVKSVTVGGPEIITVSPDSVTING
                     GEEVIVSGRNFAPESLLFLGETVISEFTVETATRIRFRVPPQIRPGVKTLTVRTRGGL
                     AQSSLFTASPRLSELQSGEITTYA"
BASE COUNT          446 a          485 c          430 g          400 t
ORIGIN      
        1 ccgcataggt tgtaatttca cccgattgta attcactcaa ccgtggcgat gccgtaaaca
       61 acgacgattg ggccaatcca cctcgggtgc gaaccgtcag ggttttgact ccaggtctga
      121 tttggggtgg cacccgaaac cgaatccggg tggcagtttc gacggtgaat tcagaaatga
      181 cagtttcgcc aagaaacagc agactttccg gagcaaagtt gcgcccgctg acaatgactt
      241 cttcaccgcc attgatggtg accgaatcag gtgacaccgt aatgatttca ggtccgccga
      301 ccgtaactga tttcacagct tcatcatccg tcatcgcatt ggcgtccagc acaaagcttt
      361 cagtgccacg gaccaccaca ctcacagcct ggctgaagct tccgggttgt gccaggtcaa
      421 agacatcggc ctgatcagtc agtgcctggg taatcacttt ggcgctggtc cggtccacga
      481 catacaggtt gccaagttcg tcaaaactca cggcttgtgg cgaatcaaac cggatttccg
      541 tacctgcctg ttttggcatt ggccaggcaa aggcaaatcg ctggcctgct tttcgagaaa
      601 gtgttcgcac cgtgccagta cggtccagga gccggaccac gttatttcct tcatcggcaa
      661 ttgccagcag ccgtccatcc gggctaattg ccccaccttt tggccggaag aagcgcgcag
      721 cacgtccggt accgtcactt ttgccgctgg tgccaggtga gccagccagc agccggattt
      781 cctgtcgggc aaagtcggca tagtacaccg cgtggttttc agtatcggca ataaagagat
      841 ttccacctgc gtcaattgcc agtccacgta acccacgaaa cacagcgcct gcaaatggcg
      901 tgacatcagc attcatcaca cccggcgccc cacttccaag cacggttgtg acctgatcgg
      961 aaaaatcaat ttttcgcacg ctgtggttgg cggtatcagc aataaagacc ccaccccgca
     1021 aactgttatt tacggccacg gcggtcggag cgttaaattt tgcctcttgc cggttgccat
     1081 tggtatttcc agtggcgcca cgttgcccgg caaaaatatc tgccggaagc gaaaaacttt
     1141 cctttttcaa aatcacgctc cccactgggt cactcaggta gatttttcct tcagcatcgg
     1201 ttttggtatc gccagggact ttggcgccac gtcctgaatg aactcgggtc accaccacaa
     1261 acgtggaaaa ggaagcctca ccccttcggg cggtaatggt ggcaaatcct tgcctgacac
     1321 cagacacccg gccagattgg gcatcaacgg aggcaatctc ggggcttcca gattcccaga
     1381 cgacattggt cacgggctgg ttactggcat caatggccgt cagggtaatc tgattttgtt
     1441 cattgacgac cggattgatt tcccggatgc gaatcccact ggattgagcc gaaacagtga
     1501 atggagctgg ttgaaattga atggagcagg tcaaaaagat gagtgaaata ctaaaccaaa
     1561 aaatcaacgg gtgtttcttc aaatgatcga atctcacaaa aacctcctgc ttgatcacaa
     1621 gcgaacggca catcaaatcg tttgtaagta gcttgccgaa aacttccaga cattttaacc
     1681 acggaaaaca cggaaaacac ggaaaacaat caagcactga ccaaatccaa tatctcagga
     1741 aacttatgac aaggtactta t
//
LOCUS       DAOQDO010001542         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71526_length_1760_cov_2.768915, whole genome shotgun sequence.
ACCESSION   DAOQDO010001542 DAOQDO010000000
VERSION     DAOQDO010001542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71526_length_1760_cov_2.768915"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PKZ53_20555"
     CDS             complement(<1..806)
                     /locus_tag="PKZ53_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42886.1"
                     /translation="MSLHQLAKALFTILTVMMVSGVIGTDSVWAQAETLPKGRGIFSV
                     FYTRSDTEKRFDFLGDRVRVIPPAGPDFRGTTKTNLLSFDVAYGITDRLEAHVTIPYA
                     NSELASIGRDGRVIKDPPEFSPSASALSNVRFGVRYNLVNEPFYLTAKFDVKLPANST
                     DLQRGFAGTTLPIDEGQTDFDITGQISKSFIVKDRTLRIGGEAGIRLRKAQKDGAIDA
                     FTNEVLPIDPANEFIYNFQVNYSLFSRLSLTLAGNGIEQGNFDVPFRTIRV"
     gene            complement(871..>1760)
                     /locus_tag="PKZ53_20560"
     CDS             complement(871..>1760)
                     /locus_tag="PKZ53_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42887.1"
                     /translation="SVTTPNGVSSENVTFTVTEKPAPGRTVELIQFGKSPDTGPDIYH
                     MTEVPDGSYVVTQYSTRSLVLLNSTGGVRQVQRNSFLLVSSIAFYSTEVAYTTCVPTI
                     LASRSFRSSDFGAEGIAPGNLGGVSNPRYMLGVDGLVSRTFFLSNGGTGEIRFIDPDT
                     RSTGRVIARGFGLSTTDANTRGLEQLAYNKTSRTIFALDSSRGVLVAVDEATGNQRVL
                     RNGFQYPFGLVLMPNGNLLVSDRGNGMLTEVTQTGQLVATYDTKQGADALRGLFVNSR
                     GEIFLLVDKTQTIFRIRFN"
BASE COUNT          461 a          465 c          448 g          386 t
ORIGIN      
        1 acccgaatcg tgcggaacgg gacgtcaaaa tttccttgct cgatcccatt tccggccagt
       61 gtgagtgaca accgactaaa caatgaataa ttgacctgga agttgtagat aaactcattg
      121 gctgggtcaa tcgggagcac ttcgttggta aaggcgtcaa tcgccccgtc tttttgggct
      181 tttctcaatc gaatcccagc ttcgccgcca atccggagcg tccgatcttt gacgataaag
      241 ctcttgctga tctgccccgt gatgtcaaag tcggtctggc cttcgtcaat cggcaaggta
      301 gttccggcaa aaccacgctg caagtcagtt gaattggctg gaagcttcac atcaaatttg
      361 gccgtcaaat aaaacggctc attgaccagg ttatagcgca caccaaaccg cacattgctc
      421 aacgcactgg ccgaaggtga aaactccggt ggatctttga tcacgcgtcc gtcacggcca
      481 atgctcgcca gttcgctgtt ggcataggga atggtgacgt gggcttccag acggtccgtg
      541 atgccatagg ccacatcaaa tgaaagcaaa ttggttttgg tcgtaccacg aaaatcagga
      601 ccggcgggag gaatcacccg cacccggtct cccagaaaat caaaccgctt ttcggtgtcg
      661 gatcgggtgt agaaaactga gaaaattccc cttcccttgg gcaaggtttc agcctgagcc
      721 caaactgaat cagttccgat gacgccgctc accatcatca cggtcagaat tgtaaaaagt
      781 gcctttgcga gttgatgaag tgacatagtg cctttcctga aaaagaaaac cgcacccaaa
      841 gaattcgaat tccctgggtg cggtggtcaa ttagttgaat cgaatccgaa agatggtctg
      901 tgttttatca acgagcagga aaatttcacc gcgtgaattc acaaacaacc cccgaagtgc
      961 atcggcaccc tgtttggtgt cataagtcgc aaccagttgg ccggtttggg tgacttcggt
     1021 caacattcca tttccacgat ctgaaaccag caaattgcca tttggcatca aaaccagtcc
     1081 gaacggatat tgaaacccat tgcgcaacac ccgttgattg ccagtcgctt cgtcaacggc
     1141 caccagcacg ccacgagaag aatcaagggc gaaaattgtc cgggaagttt tgttataggc
     1201 cagttgttcc agcccacggg tgttggcatc ggtggttgag agaccaaacc cccgtgcgat
     1261 gacccgaccc gtggagcggg tatcggggtc aataaaccgg atttcccccg tgccgccatt
     1321 tgacaaaaag aacgtacgtg aaaccagacc gtccacaccc agcatatacc gtggatttga
     1381 aacgccaccc agattccctg gcgcaatccc ttcggcaccg aaatcagaag accggaagga
     1441 acgggaggcc agaatggtgg gcacacaggt cgtataggcg acttcagtgc tataaaaggc
     1501 aattgaggaa accagcagga aggaattgcg ctgcacctga cgaactccgc cagtggaatt
     1561 gagcaacacc agtgaccggg ttgaatactg ggtcacgaca tacgagccat cgggaacttc
     1621 ggtcatgtga taaatgtccg ggccggtatc cgggcttttc ccaaactgga tcagttcaac
     1681 cgtgcgaccc ggtgccggtt tttcagtgac ggtaaacgtt acattttccg aggaaacacc
     1741 attgggcgtc gtcacactca
//
LOCUS       DAOQDO010001543         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71598_length_1759_cov_3.055164, whole genome shotgun sequence.
ACCESSION   DAOQDO010001543 DAOQDO010000000
VERSION     DAOQDO010001543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71598_length_1759_cov_3.055164"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="hemA"
                     /locus_tag="PKZ53_20565"
     CDS             complement(<1..563)
                     /gene="hemA"
                     /locus_tag="PKZ53_20565"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNJ42888.1"
                     /translation="MNLVLIGLNHKTAPVEIRERLAFGSQMLHHALHHLVDRANVEEG
                     MIVSTCNRVEVLAKTAAHPSEARHIITQFLYNYHTLPQSEVAPHLYIHEGQQAVRHVF
                     RVASSLDSMVVGEPQILGQVKDAYNEAVKAGVVGRTLYRLLPRAFSVAKRVRTETGIA
                     SFAVSLSYVAVELARKVFEDLRTKTAL"
     gene            complement(625..1434)
                     /gene="ccsA"
                     /locus_tag="PKZ53_20570"
     CDS             complement(625..1434)
                     /gene="ccsA"
                     /locus_tag="PKZ53_20570"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNJ42889.1"
                     /translation="MLLFLSLLCYGVGVVHSLWLLIRKQNVLFPVAMGAMVVGFLAHT
                     ASLVMRGIEVQRYPLMMHQEVSSFLAWAVMAYFLGAQFWYRSKSLSLFVIPVVFIFTA
                     TALLLPADQEVPSLLRFGENSPLLVVHASVFVLAYAAFVITFLAGVMYLIQERELKLK
                     RFGSFVFRLPSLDTCDDLSYKSLGIGFVLMTFGIVTGIVGSHHVAGIYWRGDPSEFLA
                     LATWIIYLFMIHCRLTAGWRGRRAALVGIVGFAMTLVSLVGVGYLSGFHFE"
BASE COUNT          489 a          486 c          432 g          352 t
ORIGIN      
        1 aagagcgcgg ttttggtgcg caggtcttca aagacttttc gagccagttc aaccgccaca
       61 taactcaacg acaccgcaaa tgacgcaatc ccggtctcgg ttcggacccg cttggcaacc
      121 gaaaacgccc gtggcagaag ccgatagagg gttcgtccga cgaccccggc tttgacggct
      181 tcgttatagg cgtctttgac ctgccccaaa atctgtggct ccccaaccac catcgaatcc
      241 aggcttgaag ccactcggaa aacatggcgg acagcctgct ggccttcatg aatatacaaa
      301 tggggtgcta cctcagattg aggcagtgtg tgataattgt ataaaaactg ggtgataatg
      361 tgtcgcgctt cgcttggatg ggccgccgtt ttggccagga cttcaacccg attacaggtc
      421 gagacaatca tcccctcttc cacattcgct cggtcaacca gatggtggag ggcgtgatgc
      481 agcatttgcg agccaaatgc gagccgctcg cgaatttcaa ccggtgcagt tttgtgattg
      541 agtccaatga gtaccagatt catagtttcg atcatgtgac gaccagcggg gatgaaaaag
      601 tctgttcgtg tgtggtgggt gacctcattc aaagtgaaag ccgcttaaat atccgacccc
      661 aaccagactg accagggtca tcgcaaatcc gacaatcccc accagagccg cccgtcggcc
      721 tcgccagcct gccgtcaggc gacaatgaat cataaagagg tagataatcc aggtcgccag
      781 ggcgagaaat tctgatgggt cgccgcgcca gtaaattccg gcgacgtggt gtgaaccgac
      841 aattccagtc acgattccga atgtcatcaa cacaaaccca atccccagtg acttataact
      901 cagatcgtcg caggtatcga gtgatggcaa ccggaacaca aaggaaccaa agcgtttgag
      961 ctttaattcg cgttcctgaa tgagatacat aacaccagcc aaaaacgtaa tcacaaatgc
     1021 ggcataggcc agcacaaaca cactggcatg cacgaccagt aatggggaat tttccccaaa
     1081 ccgaagcaac gatgggactt cctgatcagc cggtaaaagc aacgcggtgg ctgtgaaaat
     1141 aaacacgact ggaatgacaa agagcgaaag cgatttggag cggtaccaga attgagcccc
     1201 aagaaagtag gccatcaccg cccaggcaag aaacgaactg acttcctgat gcatcatcag
     1261 cggatagcgc tgcacttcga ttccccgcat gaccagcgat gccgtgtggg ccagaaatcc
     1321 aaccaccatg gcgcccattg ccaccggaaa caatacgttt tgcttacgga tcagcaacca
     1381 gagggaatga actacgccaa cgccgtaaca caacaagctg aggaaaagga gcattttcac
     1441 aggagaaatc cacatgtcgt caagttgagg atgagaagaa tccaattgta tcgctggatg
     1501 caacctgaaa caacttgcga tgcatggttt ctttgtgctt tccaggattt gcgaacacgc
     1561 ctataatgcc cacagtctcc cccggttggt tattttcagt ctcagctacg gctcaaggcc
     1621 actcgatctg gcaagggcag gaaccccagg atggcgggcc atcaccttta ccaaccacga
     1681 acggcgccac ccagcaagca caatcactgg ggatttttcg ctccacactc caacttcacc
     1741 ataaccccaa taggcaaaa
//
LOCUS       DAOQDO010001544         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71603_length_1759_cov_2.955986, whole genome shotgun sequence.
ACCESSION   DAOQDO010001544 DAOQDO010000000
VERSION     DAOQDO010001544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71603_length_1759_cov_2.955986"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="PKZ53_20575"
     CDS             complement(<1..1699)
                     /locus_tag="PKZ53_20575"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ42890.1"
                     /translation="MSPLSSAALVNFVGFLTGGTLYAMLLILVVRQPRFRGPVPHSGP
                     ATLVSPDLLPLSTALLGLFWNLGSLAVHALHDFMNGQPPAILIAGTYVSLGFLPAVVV
                     HSSLRSQPAAVATSFQRWVSVSAYGLSTVAGLIHLGSGIQGKSLPAQPALLLLTAGFG
                     VIMAALFWATRDQSGDRRAVWAVALAVFAVSALHLGQADHETESWWLALLGHQASIPL
                     AFAILYEDFRFALADIFLKRALILLTCVGLVLGVSGLGIAPLFVDHGRLDLQSPLTLG
                     VLLSLWVGSILCYPLVTRIGTWFVDSIVLRRPDYQAFCGDLARTLTTVQDPQLLLDRV
                     SHELQRALTAQNVSWVTSDVSSLDQDSTAGQPHRQLAEFPIIWHNHHAKGNFSLAEET
                     GLVTHVVFRKPQNRDRPRLPLMPFPKGQAAVLIPTVEPPNFLIVISDLSGGRRLLSDD
                     IAMLETVAVMVARRIDALKVNHERCEQNLRTQELSKLATEAELRTLRAQINPHFLFNA
                     LTTIGYLIQTSPERALETLLRLTSLLRTVLKSSGEFSTLGDELDLIESYLVIERARFE
                     "
BASE COUNT          441 a          461 c          519 g          338 t
ORIGIN      
        1 cttcaaaccg ggcgcgttcg ataaccagat aggattcgat taaatcaagt tcatcgccca
       61 gggttgaaaa ttcgccgctg gatttgagga ccgtccgcag caggcttgtt aaccgcagca
      121 acgtttccag ggcgcgttcg ggtgaagtct ggatcaaata accgatggtt gtcagggcat
      181 tgaacagaaa atgcgggttg atttgggccc gtaaggtacg tagttcggct tcggtggcaa
      241 gtttgctgag ttcctgtgtg cggaggtttt gttcacagcg ttcatggtta acctttaagg
      301 catcaattcg ccgggcaacc atgaccgcaa ccgtctcaag catcgcaatg tcatctgaga
      361 gaaggcggcg gcctccggaa agatccgaaa tgacaatcag aaagtttggc ggttcaacgg
      421 ttggaatcag aacggctgcc tgtcctttgg ggaagggcat gaggggaagc cgtggccggt
      481 cacggttctg tggtttccga aagacgacat gagtgaccag gccggtttct tcggccaatg
      541 aaaagttccc tttggcgtgg tggttgtgcc agatgattgg aaattcagcc agttgccggt
      601 gaggctgtcc ggcggtcgag tcttgatcca gtgaactgac atcagaggtc acccaactga
      661 cattttgggc ggtcagcgcc cgctgaagtt cgtgtgacac ccggtcgagc aaaagctgtg
      721 gatcttgaac cgtggtcagg gtgcgggcca aatcgccgca aaatgcctgg taatctggtc
      781 gccgcaggac aattgaatcc acaaaccagg tgccgattcg agtgacgagc gggtagcaga
      841 ggattgaacc aacccaaaga ctcagcagca cacccagcgt gagcggtgat tgcagatcaa
      901 gcctgccgtg atcaacaaac agcggcgcga tacccagacc actgacaccg agaaccagtc
      961 caacacaggt gagcagaatc aacgcccgtt tgaggaaaat atcagccagt gcaaaacgga
     1021 aatcttcata caaaatagca aaggcaagtg gaattgacgc ctgatgccca agcaatgcca
     1081 gccaccacga ctctgtttcg tggtcagcct ggccaaggtg cagtgccgaa accgcaaaca
     1141 ctgccagggc gaccgcccac acggccctcc ggtcacctga ctggtcgcga gtcgcccaaa
     1201 acagagccgc cataatgacg ccaaatccag cggtcagtaa caggagtgcc ggttgggccg
     1261 gaagggattt tccctggatt ccgctgccca gatgaatcag cccggccact gtactgagcc
     1321 catacgctga gacactgacc caccgttgaa aactggttgc caccgccgcc ggttgcgaac
     1381 gcaatgacga atgcaccacg acggctggta gaaatccaag ggaaacatag gttccggcaa
     1441 tcaaaatggc gggaggctgg ccgttcataa aatcgtgcag cgcgtgaacc gccagtgatc
     1501 ccagattcca aaacagcccg agcagggccg tactcaaggg gagcaaatca ggtgaaacca
     1561 gagtcgccgg tcctgaatga ggcaccggtc ctcgaaatcg gggttgacgc acaaccagaa
     1621 tgagcagcat ggcatagagc gtccctccgg tcagaaaacc aacaaaattg accagtgctg
     1681 ctgaggaaag tggtgacatg ggggttttag ggttccgggt tccgggttcc gggttccggg
     1741 ttccgggttc cgggttccg
//
LOCUS       DAOQDO010001545         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71784_length_1757_cov_2.327850, whole genome shotgun sequence.
ACCESSION   DAOQDO010001545 DAOQDO010000000
VERSION     DAOQDO010001545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71784_length_1757_cov_2.327850"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKZ53_20580"
     CDS             complement(<1..529)
                     /locus_tag="PKZ53_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42891.1"
                     /translation="MPQPKILIVDDNAKNRAICEEILMDEYELCQAVDGVEALEKVPV
                     FQPDLILLDLMMPRMDGYEVCRRLKSDPHTTHVMIVCVSAKGQTAEIVEGFQAKADDY
                     IVKPFSEEELLARVRAALRLKLTQDELKRVNTNLEEIVAKRTAQLIEAERLAVIGRNV
                     AQMAHNFRGALTTITL"
     gene            complement(653..>1757)
                     /locus_tag="PKZ53_20585"
     CDS             complement(653..>1757)
                     /locus_tag="PKZ53_20585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNJ42892.1"
                     /translation="LNNLVEVVYSRGPLPNFEMVLISPSIERLIGHQATEFSARPNLI
                     LDLVHPDDQPDVQAKLTGLFENGIEFIAEYRIQNDQTCTWCWVRDHLRPRFSPAQQIV
                     GYDGVLVDITERRQLEADIALARDRAEEANRLKSEFLSNISHELRTPLHGILSYARFG
                     LKRYDKVEREKLRAYFDEIRDSGEHLLGLINDLLDLSKIEAGKMDFEFQPLPIGHIVD
                     GVYSKLIHLVESKNIDFVCPPIPEEWIVEGDQNKLLRVFINLVGNSVKFTPVGGKIIL
                     NAEEATPASYCISVSDTGQGIPPEDLEHIFDKFAQSRHDPTHHFSGTGLGLAICREII
                     AIHGGRIWAESPGLGQGSTFYFTLPKTRQENVK"
BASE COUNT          400 a          445 c          445 g          467 t
ORIGIN      
        1 aaagagtgat ggtggtgagg gcaccacgaa agttatgcgc catctgggcg acattgcgac
       61 caatcaccgc cagtcgctcg gcttcaatca actgggcggt gcgtttggcg acgatttctt
      121 caaggttggt attgacccgc tttaattcgt cctgggtcag tttcaatcga agtgccgccc
      181 ggactcgggc cagtaactcc tcttcgctaa atggtttcac aatatagtca tcagctttgg
      241 cctgaaatcc ttcaacaatt tccgctgtct gccccttggc tgagacacaa acaatcatca
      301 catgtgtcgt atgcgggtca gatttcagcc ggcggcagac ttcatagcca tccatgcggg
      361 gcatcatcag gtccagcaga atcaaatcag gttgaaacac cggaactttt tccagagctt
      421 cgaccccatc cacggcctga cagagttcat attcgtccat caggatttct tcgcagatgg
      481 cccggttttt ggcattgtcg tcaacaatca gaattttggg ttgtggcata tgatgagggt
      541 tcggggttca gggttccggg ttccggattc cggattccgg gttttcgaat tttgatcttt
      601 tttgtccctt ttgtctttgt tgtattttca aaacctgacc ccctgtactg gtttacttta
      661 cattttcctg gcgtgttttc gggagggtga agtaaaacgt actgccctgt ccgagtcctg
      721 ggctttcggc ccagatgcgg cctccgtgga tggcgataat ttcacggcaa attgccagcc
      781 ctagccctgt accgctgaaa tgatgggtcg ggtcgtggcg actttgggca aatttgtcaa
      841 aaatatgctc caggtcctct ggcggaattc cctggccggt atcggaaacg gaaatacaat
      901 aacttgccgg ggtggcttcc tcggcgttca ggatgatttt tcctccaacc ggagtaaatt
      961 tcaccgagtt tcccaccaga ttgataaaga cccgcaggag tttgttttga tcaccttcga
     1021 caatccattc ttcagggatt ggcggacaca caaagtcaat gtttttgctt tcaaccagat
     1081 ggatcagttt ggaatagaca ccatccacaa tatgcccaat cggaagcggt tgaaactcaa
     1141 agtccatctt gccggcttca atttttgaca ggtcgagcag gtcgttgatc aacccgagca
     1201 gatgctcacc gctatcgcga atttcatcaa agtaggctcg cagcttttcg cgctcgactt
     1261 tgtcataccg cttgagccca aaccgggcat agctcaggat gccgtgaagt ggtgtgcgca
     1321 gttcgtgtga gatattggac agaaattccg atttcaggcg gttggcttcc tcggcgcgat
     1381 ctcgggccag ggcaatgtca gcttcgagct ggcgacgttc ggtaatgtca accagcaccc
     1441 catcataccc gacaatctgt tgagcgggac taaaccgagg tcgtaagtga tcgcgcaccc
     1501 agcaccaggt gcaggtctgg tcattttgga tgcgatattc ggcgataaat tcaatcccat
     1561 tctcaaacaa tccggtgagt ttggcctgaa cgtccggctg atcatcagga tgaaccaggt
     1621 ccagaattag atttggtcga gcagaaaatt ctgtcgcctg atgaccgata agccgctcaa
     1681 tactgggact gatcaaaacc atttcaaaat tcggcaacgg gccgcgactg tacacgactt
     1741 caaccaggtt attgagt
//
LOCUS       DAOQDO010001546         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71836_length_1756_cov_2.952381, whole genome shotgun sequence.
ACCESSION   DAOQDO010001546 DAOQDO010000000
VERSION     DAOQDO010001546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71836_length_1756_cov_2.952381"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1756)
                     /locus_tag="PKZ53_20590"
     CDS             complement(<1..>1756)
                     /locus_tag="PKZ53_20590"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ42893.1"
                     /translation="DEITTTGSLTGQLQFPGQTQQLKKDGKTTQEIEMERQGTQKLTP
                     QPQQTQQLQMKSSGSTVRMATMRLQRGQTGMLTALAGLSGDEQKKFQESIDTFWERRK
                     QAEQGEQKLADISALRPQEPDPDLAQYNLHLGKKQFNWLPSFDLVRPWPRGYLYVFGG
                     AAILAMILAAVVYEKAYSPGEVSNPHNLPLAQQPTDTSQRVGAKIVANIAAENNCAAC
                     HSPVLSMQKLCMDCHQTAHFNPEVISAHQKANVGCVDCHTEHKGQEFKPAGVARSMCI
                     DCHRDDPRHPKAHDAKGQLLKNPHPNTAGYPVNRQTGQWTWTDPLNPKWKQVFPANIG
                     PKEKFHLVHGVGLNAAGEDWKCSGCHEGFTPAAKAKVISEEKCSVCHGATYDEESKRK
                     LDQLAVNFKLDCNSCHPQHGYDTVALASLKKAGKDRTPIVWTPEGVYRGGKDWQWTSF
                     AAKFGGMSVNGWLAFFSILPLTALGFLAVDSNRRRNVLNLLKGNIIELSRKKVKFNDI
                     TYAKRWESKEQIKRAEEAARARPVPQPVINYETCIGCHNCILACPQDVLGFDDQEHHA
                     VVVNYEQCMEDTGCQQACP"
BASE COUNT          332 a          452 c          501 g          471 t
ORIGIN      
        1 tgggcaggct tgctgacaac cggtgtcttc catgcactgt tcatagttca ccacaacggc
       61 atggtgttcc tgatcgtcaa agcccagcac atcctgtgga caggccagaa tacagttatg
      121 acacccgata caggtttcat agttaatgac gggttgtggc acggggcggg ccctggcggc
      181 ttcttcagcg cgtttaattt gttctttgga ctcccaccgt ttggcatagg taatgtcgtt
      241 gaattttacc ttcttgcgag ataattcaat gatgttgcct ttgagcaggt ttaagacatt
      301 ccggcggcgg tttgaatcaa cggccaggaa cccaagcgcg gtgaggggaa ggatgctaaa
      361 gaaagccagc cagccattga ctgacatccc accgaatttg gccgcaaaag acgtccattg
      421 ccagtctttc ccgccccgat agacgccctc tggtgtccag acaatcggcg tgcgatcttt
      481 gccggctttt ttgagcgaag ccagcgccac cgtgtcataa ccgtgctgtg ggtggcaact
      541 attacaatcc agtttgaaat tgaccgccag ttggtcaagc ttgcgtttgc tttcttcgtc
      601 ataggtggca ccgtggcaaa cgctgcattt ctcttcactg atgaccttgg ccttggccgc
      661 cggggtgaag ccttcgtggc aacctgagca tttccagtct tcgccggcgg cattgagtcc
      721 gacaccgtga accagatgga atttttcttt cggcccaatg ttggccggga agacctgttt
      781 ccactttgga ttgagggggt cggtccaggt ccattgaccc gtttgccgat tgaccgggta
      841 gccagcggtg ttgggatgcg gatttttcag taactggcct ttggcatcat gggcttttgg
      901 atggcgaggg tcatcgcggt ggcagtcaat gcacatactg cgggcgactc cagccggctt
      961 gaactcctgg cctttgtgtt cggtgtgaca atcaacgcac cccacattgg ctttctggtg
     1021 ggcggaaatg acttctggat tgaagtgagc ggtttggtgg caatccatac acagtttctg
     1081 catggagaga actggcgaat ggcaggcggc acaattgttt tcagccgcaa tgttggccac
     1141 aatcttggcc ccaacccgct gactggtatc agttggttgc tgggccagcg gcaggttatg
     1201 cggattagaa acttcgccgg gtgaataggc tttttcatag accacagcgg ccaggatcat
     1261 cgccaggatc gcggctcccc caaaaacata gaggtatccg cgtggccagg gacgaaccag
     1321 atcaaatgac ggtagccagt taaattgttt tttgcccaga tgcaggttgt attgtgccag
     1381 gtccggatcg ggttcttgcg ggcgcaaggc cgaaatatcc gccagctttt gctcgccctg
     1441 ctcagcctgt ttgcggcgtt cccagaacgt gtcgattgat tcctgaaatt ttttctgttc
     1501 gtcgcctgaa agcccggcca gcgccgtcag catcccggtc tggccgcgtt gcagccgcat
     1561 ggtggccatg cggacggtgc tgccgctgct cttcatttgc aactgctggg tttgttgagg
     1621 ctgtggggtc agcttttgtg tcccctgacg ctccatttca atttcctggg ttgtttttcc
     1681 atctttttta agctgttggg tttgtcctgg aaattgaagt tgtccggtca ggctcccggt
     1741 ggtggtgatt tcatcc
//
LOCUS       DAOQDO010001547         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71904_length_1755_cov_3.507647, whole genome shotgun sequence.
ACCESSION   DAOQDO010001547 DAOQDO010000000
VERSION     DAOQDO010001547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71904_length_1755_cov_3.507647"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKZ53_20595"
     CDS             complement(<1..198)
                     /locus_tag="PKZ53_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42894.1"
                     /translation="MSRDGDRPLIFFWYNLALVVGLPLVLPYYVFRAIRHGKYLPGFK
                     ERLGYLPSALRFSRGPVKSVIW"
     gene            complement(223..780)
                     /gene="efp"
                     /locus_tag="PKZ53_20600"
     CDS             complement(223..780)
                     /gene="efp"
                     /locus_tag="PKZ53_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNJ42895.1"
                     /translation="MRANQIRRGTIIIFNGEPHRVVEFRHHTPGNLRAMVQTKLKSVK
                     TGSAFEHRFSATEDVERATLEQHDMNYLYSDGTTHHFMNNETYEQIGLDEEMLGDAIY
                     YMTPDLTIQVEFFDGSPIGISLPPSVELRVAETDPELKGATVSGSSKPAKLETGLTVN
                     VPGFIKEGELIRVDTTEGTYIERVK"
     gene            1159..>1755
                     /locus_tag="PKZ53_20605"
     CDS             1159..>1755
                     /locus_tag="PKZ53_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42896.1"
                     /translation="MHYKVIFERAGDGSPQAQALVHDRLITHFKVSPEVATAMLAQAP
                     LVVKQGLTQDQAEAYQQALESIGVVASIEVTFSLSEAPTAPNPPQPASAPVPPLIPSS
                     ATMGGLESEIAAVMSGSTQISDPPRSGWSPSVSPSQPPSSGTVQLPPNQPSPPTPAPA
                     ISLGKPKENPPAPAIGNTADWWARIRVCNPPFNEAGRGM"
BASE COUNT          446 a          479 c          429 g          401 t
ORIGIN      
        1 ccaaataaca gatttgactg ggccacgcga aaatcggagg gcagaaggta aataccccag
       61 ccgttcttta aaccctggta gatatttgcc atggcggatt gcccgaaaca cgtagtaagg
      121 caacaccagt ggtaacccta ccaccaatgc caggttgtac cagaaaaaga tcaacggacg
      181 atcaccatca cgggacatgt gaatgctgac aacaaactgg tcttatttga cgcgttcgat
      241 gtaagtacct tctgtggtgt ccacccggat caactcgcct tccttgatga accctggcac
      301 gttgaccgtt aaaccggttt ccagttttgc tggtttggag gaaccgctga ccgttgcgcc
      361 cttcaattct ggatcggttt cggccactcg cagttcaacg gatggtggca atgaaatccc
      421 aatcggactc ccgtcaaaaa attcaacctg aatggtgaga tcaggggtca tgtaatagat
      481 cgcatcgccc agcatttctt catcgaggcc aatttgctca taggtttcat tgttcatgaa
      541 atgatgagtt gtgccatcgc tgtagagata gttcatatcg tgctgttcca gcgttgcccg
      601 ttcaacatct tcagtcgcac tgaatcggtg ttcaaacgcg gacccggttt tgacgctttt
      661 gagcttggtt tgcaccatgg cgcgcaggtt gccaggggta tggtgacgga attccacaac
      721 gcgatgcggt tctccgttaa aaataataat tgtgccacga cgaatctggt tcgctctcat
      781 aacaaatcga aaataactcc ttacagaaat tacggcggaa atcgccagga ttcttgaaaa
      841 gagcacagcc tagatgagtt accggaaacg gtcaagtcaa gcgaagccag tcaggtcaga
      901 atttacagcg agatagcctg tgggcggaaa gaaaatggtt caccagtttc ccggcaatca
      961 catctcaacc cagttccccc tgaccaccac acctgagcaa agagctaaat aattgggaga
     1021 actgtatttt cccaaaccct gaaccctgaa ccctgaaccc tggattggta taaggttgca
     1081 gttttgtaaa agtagcttac acttgggggg tctctgtccc aaaagatccg aactcttcaa
     1141 ggccaaccac tcactgtaat gcattataaa gtcatcttcg aacgcgccgg ggatgggtcg
     1201 ccccaggccc aggcgctggt tcacgaccgg ttgatcaccc atttcaaagt gtcacctgaa
     1261 gtggcaaccg cgatgcttgc gcaagcaccg ctggttgtta agcaggggct cacccaggat
     1321 caggctgagg catatcagca agcccttgaa tcaattgggg ttgtagcatc aattgaagtg
     1381 acatttagcc tgtcagaggc cccaacggcg ccaaatccgc cccaaccagc gtcagcaccg
     1441 gtgccacctc tgattccttc ctcggcaact atgggcgggc ttgagagtga aattgcggct
     1501 gtgatgagtg gctccaccca aatctcagat cctcctcggt caggctggtc tccatctgtt
     1561 tcaccctctc agccgccatc ttctggaacc gtccagctcc cgcccaatca accatctccc
     1621 ccaacaccgg cgccggccat atcgcttgga aaaccgaaag aaaatccacc ggcgccagct
     1681 atcgggaata cagccgactg gtgggccaga atccgggtct gtaacccgcc ttttaatgaa
     1741 gccgggcggg ggatg
//
LOCUS       DAOQDO010001548         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_71912_length_1755_cov_2.832353, whole genome shotgun sequence.
ACCESSION   DAOQDO010001548 DAOQDO010000000
VERSION     DAOQDO010001548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71912_length_1755_cov_2.832353"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PKZ53_20610"
     CDS             <1..1462
                     /locus_tag="PKZ53_20610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ42897.1"
                     /translation="FVQDDWKVRTGLTFNLGLRYELNTVPTASGRLTNFTPDRGFFND
                     QAGLFDGDHNNFAPRIGFAWTPFEFGRTVIRGGAGMFYDLVFANVPFSLTFNPPASVQ
                     FYPLFGTDPEPGQLGSVFADIPQAPDFEDAGPALITIDPKLRTPYAFQWNLNVQQELG
                     NGLGFEIGYYGTRGVKQVLNRDINQAVFFPGDSSVSNIFDRRPTQLSGNAFLLNGVDV
                     GGIDLIQQQEASATSIYHSLQTRLSGRLTKDFYFLTSYTWAHSIDNASDIFGFTGSSG
                     FPQDSNNLRAERANSPFDIRHRFTSSFTFDLPFGQGKMFGSKASRKLDLVIGGWQVNG
                     IITAQTGQPFSVRLGQDVALDGNPLNEQRPNDIPGAFVPDGKGGLRLTVPVERLIPSP
                     GTYGNLGRNTFRGPKFVNFDFSVFKEFRLKDDIKLQFRSEFFNLFNHPNFALPEVNLS
                     SPTFGTFSRTPDVAAGSPRIASGAQRVIQLALKLTF"
BASE COUNT          413 a          443 c          440 g          459 t
ORIGIN      
        1 ctttgtccag gatgactgga aggttcgcac cggcctgact ttcaaccttg gattgcgcta
       61 tgaactcaac accgtcccca ccgccagtgg ccgattgacc aatttcacgc cggaccgggg
      121 tttcttcaat gatcaggctg gattatttga cggtgaccac aacaactttg cgccgcgaat
      181 tgggtttgcc tggacgccat ttgaatttgg aagaacggtc atccggggcg gggccgggat
      241 gttttatgac ctggtttttg ccaatgtacc gtttagcctg acctttaatc cgccagcgtc
      301 agttcagttt tatccgttgt ttgggacaga tccggaaccg ggtcagcttg gttccgtttt
      361 tgccgatatt ccgcaagcac cagattttga agatgctggg ccggcattga ttacgattga
      421 cccaaaactc cgcacgccct atgcctttca gtggaatctg aatgttcaac aggaactggg
      481 caatgggctg gggtttgaaa tcggctatta cggcacccgt ggcgtcaaac aggtactcaa
      541 tcgcgacatc aatcaggccg tctttttccc tggcgattcc agcgtttcaa acatttttga
      601 ccgccgacca acccagttga gcgggaatgc ctttttgctc aatggcgtgg atgtcggcgg
      661 aattgatttg attcagcagc aggaagcgtc ggcgacttcg atttatcatt cgctccaaac
      721 ccgactgagc ggacggctga ccaaagattt ctactttttg accagttaca cctgggcaca
      781 ttcgattgac aatgcctcgg atatctttgg atttaccggg agttcggggt ttccacagga
      841 cagtaacaat ttgcgagccg agcgagccaa ttcaccattt gacatccgac atcggttcac
      901 cagcagcttc acctttgatt tgccttttgg gcaggggaaa atgtttggtt caaaagccag
      961 tcgaaagctt gatctggtga ttggcggctg gcaggtcaac ggcatcatca cggcgcaaac
     1021 gggccagccg ttttcggttc ggcttgggca agacgtggca cttgatggca atccactcaa
     1081 cgaacagcgc ccaaatgaca ttccgggtgc ctttgttcca gatggaaagg gtgggttacg
     1141 cttgacggtt ccagtcgaac gtcttattcc gtcacctggt acctatggaa atctgggccg
     1201 aaatacattt cgcggtccaa aattcgtcaa ctttgatttt tcagtattca aggaattccg
     1261 cctcaaggat gatatcaagc tccagttccg ttcagagttc tttaacctgt ttaatcatcc
     1321 gaatttcgct ttacctgaag tgaatctctc atctccaacc tttggcacgt tttcgcgaac
     1381 tccggacgtt gcggctggca gcccacggat tgcctctggt gcccagcggg ttattcaatt
     1441 agccttgaag ttgacgtttt agtacctggt gcttcgtgct tggttcttgg tgactggttc
     1501 ttcttcgaaa gtattgattc ctaagcacaa agcaccaggc acaaagaaca aagcacaaag
     1561 cacaaagaac aaagaacaaa gaacaaagca caaagcacaa agcacaaaga acaaagcaca
     1621 aagcacaaag caccacactc catgaaagca ggaccaccta tgaaccgggg aatttgcgtt
     1681 tgttggccac gcgccttttc cctgggtgga tatctgcttt ggttgtgtct ggcactctgg
     1741 tggattggtg accag
//
LOCUS       DAOQDO010001549         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72059_length_1753_cov_3.212603, whole genome shotgun sequence.
ACCESSION   DAOQDO010001549 DAOQDO010000000
VERSION     DAOQDO010001549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72059_length_1753_cov_3.212603"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PKZ53_20615"
     CDS             <1..1614
                     /locus_tag="PKZ53_20615"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ42898.1"
                     /translation="LAVFWIQPAVCAQTRSVSNQSGKFQVFVKGKPVGTEIFHITGDE
                     YTGEVSIDLGVKVNGKSRLRYRGNQIEFFEAEQNNARLSLDTSTTPMKLTSPAGTREV
                     IIPDGAILLETVYLHQYHALVNRYDLEKGGKQSFQTFVPTIGMVFPLTCELTERIEPA
                     PPNQPMTLLKFQTNVANVQVINLITDENRSILMIDVPAQGYTVVREGFESLRPRSETE
                     IVSDKYDRIEVEIPTTNGVKLAGTLTVPKNGNSRHPVVVLITGSGPQDRDEDTPPFIN
                     YHLFRLIADRLSHNGIATLRCDDRGVGKSTGDFLSAGFHDFIADARAQVEFLRKRPEI
                     DPARISVLGHSEGGYTAPLLAATDSKLKAVVILAGPSKRLDPLMIEQLEYQAGFQDLP
                     EGTRSYARSLIDATKKMIADVKANKSVTDKEGRKLVKVGRLQWNIDYFRQHFAHDPVK
                     TIRKVRCPVLILQGDRDVLVQKYHAEALAAELKQAGNKNVTLHIFPYLTHMFTTFPYN
                     NPDPKALSKSETVSDEFLEALKDWGIKNF"
BASE COUNT          424 a          436 c          452 g          441 t
ORIGIN      
        1 ctggcggtat tttggattca acccgccgtt tgcgcccaga cacgatcagt gtcaaatcag
       61 tcgggaaaat ttcaggtttt cgtcaaaggc aaacctgttg ggacggaaat ctttcacatc
      121 acaggggatg aatataccgg cgaagtctcg attgatctag gggtcaaggt caatggcaaa
      181 tccaggttgc gttatcgtgg aaaccagatt gagttctttg aagccgaaca aaacaatgcc
      241 agattgtcgc ttgatacctc caccacgccg atgaaactca ccagtccggc tggaacccgc
      301 gaagtcatca tcccggatgg cgcgattttg cttgaaaccg tgtacctgca ccagtatcac
      361 gccctggtta accgatatga ccttgaaaaa ggtgggaaac agtcgtttca aacctttgtg
      421 ccgacgattg gcatggtgtt tcccctgacc tgcgaattga ccgaacggat tgaaccagcg
      481 ccgccaaacc agccaatgac gctgctcaaa tttcaaacca atgttgccaa tgtgcaggtg
      541 atcaacctga ttaccgacga aaaccgctcg attttgatga ttgatgttcc ggctcagggt
      601 tacacggtgg ttcgggaagg gtttgaatca ctccgccccc ggtctgaaac tgagattgtc
      661 agtgacaaat atgaccggat tgaagtggaa atcccaacta ccaatggcgt caaactggct
      721 ggaaccttaa ccgttcccaa aaacggcaat tcccgccatc cggttgtggt ccttatcaca
      781 ggctctggtc cacaggatcg ggatgaagat acgcccccat ttatcaacta ccatctcttt
      841 cggctgattg ctgatcgact ctcccacaat ggaattgcca cgttgcggtg tgatgatcgc
      901 ggcgtaggca aaagcaccgg ggattttctc agcgccgggt ttcacgattt tattgctgat
      961 gcccgggccc aggtggagtt tctcagaaaa cgcccggaga ttgacccggc ccgaatcagc
     1021 gtccttggac atagtgaagg cggctatacg gcgcccctgc tggcagccac cgattcaaag
     1081 cttaaagcgg ttgtcatttt ggccggtcca tccaaacggc tggatcccct gatgattgaa
     1141 caacttgaat accaggccgg gtttcaagat ctaccagagg gcacacgaag ctatgcccgg
     1201 tctttgattg atgccaccaa aaaaatgatt gccgatgtga aagccaacaa atcagtgact
     1261 gacaaagaag gccgaaagct ggtgaaagtt ggacgcttac agtggaatat tgactatttt
     1321 cggcagcatt ttgcccacga tccggtgaag acgattcgca aagtccggtg cccggtgctg
     1381 attttgcagg gagaccggga tgtgttggtc cagaaatacc atgccgaagc cctggccgcc
     1441 gaactcaagc aagccgggaa caaaaacgtc acgcttcaca tctttcccta tttgacccat
     1501 atgtttacca ctttcccgta caacaacccc gatccgaaag cactttccaa atctgaaacg
     1561 gtttcggatg aatttttgga ggcgctcaag gactggggaa tcaaaaactt ttgagatggt
     1621 aactggtgac tggtgactgg tgactggttc ttggttcttg gttcttggtt cttggttctt
     1681 ggtgcttggt gcttggtgct tggtgcttgg ttcttggtgc ttggttcttg gttcttggtt
     1741 cttggttctt ggt
//
LOCUS       DAOQDO010001550         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72079_length_1753_cov_2.450530, whole genome shotgun sequence.
ACCESSION   DAOQDO010001550 DAOQDO010000000
VERSION     DAOQDO010001550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72079_length_1753_cov_2.450530"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKZ53_20620"
     CDS             complement(<1..594)
                     /locus_tag="PKZ53_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HNJ42899.1"
                     /translation="MAKLPVTLEHLTESFGPHLARNAGNRLATGIEPDSVVKTHCCFC
                     GQQCGIQLKVKDNEVIGFEPWEEFPFNRGMLCPKGVKRYLQGAHPDRLLTALKRDPSH
                     PSGFSPMPYAEAIDRVATEIKRIQAVHGRDAFALLSGASLTTEKAYLMGKFARVALKT
                     ANIDYNGRLCMVSAGAGNKKAFGIYRAANPWSDMLKTD"
     gene            complement(824..1618)
                     /locus_tag="PKZ53_20625"
     CDS             complement(824..1618)
                     /locus_tag="PKZ53_20625"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ42900.1"
                     /translation="MPKKIAVVSVKGGVGKTTTCINLAAYTAAMGVNVLLVDLDPQNG
                     CLYGMGLEFRPGYSLKDAVLNGVHPGNALSSTNRPELNVLLNGFFETGEQLEAFQAAF
                     EQDVYLLDTLLTALTQERDLTPDLILMDCPAGVGAITLNALVTADSVILPMQCEPLSL
                     RTLPQMLRSVASVKSRYNPQLVVEGVLATMFDPRQEAKFGIAQQVWTDFPEDMVFETV
                     IPRHDQLIESFAIGAPAIDISARSMGSQAYIQLGRELIMRLPEETG"
BASE COUNT          422 a          450 c          443 g          438 t
ORIGIN      
        1 atccgttttc aacatatcgg accagggatt ggctgctcga taaattccga aagctttttt
       61 attgcccgcc ccggcgctga ccatacagag ccgcccgtta taatcaatat tggccgtttt
      121 gagcgcgaca cgggcaaatt ttcccatcaa ataggctttt tcagtggtca ggctggcccc
      181 gctgagcagg gcaaatgcgt ctcgtccgtg gacggcctga atacgcttga tctctgttgc
      241 cacccggtca atcgcttcgg catagggcat cgggctaaac cctgacggat gtgacggatc
      301 ccgtttcagt gccgtaagta gccggtctgg gtgggcgccc tgcaggtacc gcttcacgcc
      361 cttggggcac aacatcccac gattgaatgg gaactcttcc cagggttcga agccaatcac
      421 ctcattatct ttgactttga gctggattcc acattgctgc ccgcaaaaac agcagtgtgt
      481 tttcacgacc gagtctggtt caattccggt tgccagccgg ttgccagcat tgcgagccag
      541 atgtggacca aatgattcgg tgaggtgttc gagtgtaacc gggagtttag ccatacgttt
      601 tttgtaatac cagtcagtcg tcaatagtca gtagttagta gtttgttaag tttacctggc
      661 agaatcactt aactgtttgc ttgtccaggg atcttatcac aaattggtat aatttgtgta
      721 tgcactggtt aggatagaac cagggccagg ggctaagggc tgaagaagtg gttttatttc
      781 atccctcatc cttcaccctt catcccttca attgcccctt tcttcaacct gtttcctctg
      841 gcaaccgcat aatcaattcc cttccaagct ggatatatgc ctgtgatccc atcgaacggg
      901 ctgaaatatc aatcgccggg gccccaatcg caaatgactc aatcaactgg tcgtgacgag
      961 gaatcacggt ttcaaaaacc atgtcctctg gaaaatcagt ccacacctgt tgggcaatgc
     1021 cgaattttgc ttcctgacgc ggatcaaaca tggtggccag gacaccttca accaccagtt
     1081 gcggattgta cctggattta actgaagcca ctgagcggag catctggggc agggtccgta
     1141 aactcaatgg ctcacattgc atggggagaa tcactgagtc agccgtgacg agggcattga
     1201 gggtaatggc gccaaccccg gcgggacaat ccatcaggat caaatcaggc gtcaggtctc
     1261 gctcctgagt cagggccgtc aacagagtat ccagcaagta cacatcctgt tcaaaggcag
     1321 cctgaaacgc ctcaagttgc tctccggtct caaaaaaacc atttaaaaga acattcagtt
     1381 ctgggcggtt cgtggatgac agtgcgtttc cagggtgaac accattcaga acagcatcct
     1441 ttaaactgta ccctggacga aattcaagcc ccattccgta caaacagcca ttttgtgggt
     1501 caaggtcaac gagcaaaaca ttgaccccca tggccgccgt gtaggcagct agattgatac
     1561 aggttgtggt tttcccaaca ccacctttta cactcaccac agcaattttt ttcggcatag
     1621 gtgtttcctg aagatttttg ggctgaagat tttcgggctc aggactcgga actgaagacg
     1681 tcgggctcag ggctcggagc tgaagactcg caagctcggg gcaattgaag ggatgaggga
     1741 tgaaggatga ggg
//
LOCUS       DAOQDO010001551         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72145_length_1752_cov_2.644667, whole genome shotgun sequence.
ACCESSION   DAOQDO010001551 DAOQDO010000000
VERSION     DAOQDO010001551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72145_length_1752_cov_2.644667"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..>1752
                     /locus_tag="PKZ53_20630"
     CDS             45..>1752
                     /locus_tag="PKZ53_20630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HNJ42901.1"
                     /translation="MTPINSHLTPNEVSYIIQDSDAKALFFNSKTADVCTQAIQAIGY
                     PSHLCFSTDGHPKFPSYQELKNSQPTTLPSLRYAGASMTYTSGTTGKPKGVQISHRAL
                     VNFLCSMQREPGISSQDILVAVTTLSFDIAGLELYLPLLAGATVVIASRAVAMDGHRL
                     ARLLDVSQATVMQATPTTWRMLIEASWSPSLPFKALCGGEAFPPDLAQLLLERTTEVW
                     NLYGPTETTIWSTCLKLEPNQPISLGRPIANTQVFILDQSLHPVPIGIPGELCIGGDG
                     LARGYLNRPELTAERFKTGVTGPGSGVRGSGATEPDQPEGQREESLEPTSQETWGRSE
                     TQGQTLNPVPRTLYRTGDLARWRPDGTIEYLGRLDFQVKVRGFRIELGEIESALSAHP
                     AVKQAAVVVQKTDGGDQQLTAFLILTDSSPDTSQLRSFLKQSLPDYMLPSIFVVLEDF
                     PLTLNGKIDRKALTTHQGGRWHQVTRQVPPRTLVEFQLLTLWQHLFHRQSISIEDDFF
                     QLGGHSLMAIRLAAAIEKEFGCRLPLAILFQEPTIARLAAAVRTMSPDREWSPLVTLQ
                     PRG"
BASE COUNT          410 a          506 c          420 g          416 t
ORIGIN      
        1 ctatgcttga attgtaccta gctgctactc aagcagggtt ttatatgaca cctatcaata
       61 gtcatctcac accaaatgaa gtatcttata ttattcaaga ttcagatgcc aaagcactct
      121 tctttaactc aaaaacagcc gatgtctgca ctcaagcgat ccaagccata gggtatccat
      181 ctcatctttg tttttcaacg gatggacacc ctaaatttcc atcctatcaa gaacttaaaa
      241 actcacaacc tacgactctt ccttctcttc gttatgcagg agcaagcatg acttatacct
      301 ctgggacaac aggcaaaccc aaaggggtcc agatttcgca tcgggctctt gtcaactttt
      361 tatgttctat gcaacgggaa cctggcattt ccagccagga catcctggtg gcagtgacga
      421 cgctttcctt tgacattgcc gggcttgaac tgtacctgcc gctcctggct ggcgccacag
      481 tcgtcattgc cagccgggca gtggcaatgg atggccaccg cctcgcccga ttgctcgacg
      541 tgtcacaggc tacggtcatg caggcaactc ccaccacctg gcgaatgctc attgaagcca
      601 gttggagtcc gtcacttccc ttcaaagccc tctgtggcgg cgaagcattt ccccctgatt
      661 tggcccaact cttgctggaa cggaccactg aagtctggaa tctctatggt ccaaccgaaa
      721 ccaccatctg gtccacctgc ctcaaacttg aacccaacca gccgatttcg ctcggtcgtc
      781 ccattgccaa cacccaggtt ttcattcttg accagtctct ccaccctgtt ccaattggga
      841 ttcctggtga actttgcatt ggcggtgatg gactggcacg cggatacctg aatcgcccag
      901 aattgacggc ggagcgattt aaaaccgggg tcacgggtcc ggggtcaggg gtcaggggtt
      961 ctggagcaac agagcctgat caacctgagg gccagcgtga agaaagtttg gagccaacct
     1021 cccaggaaac ctggggacgt tccgagactc aaggccagac cctgaaccct gtccctcgaa
     1081 ccctgtatcg aaccggggat cttgcccgat ggcggccaga tggaacaatt gaatacctgg
     1141 gacgccttga ttttcaggta aaggtccgtg gatttcggat tgagctgggc gagattgaat
     1201 cagcactcag cgctcacccg gctgtcaaac aagcagcagt cgtggttcaa aaaacagatg
     1261 gcggtgatca gcaattgacg gcttttctga ttctgaccga ctcctcacct gacaccagcc
     1321 agctccgttc attcctgaaa caaagcttgc ccgactatat gcttccttcc atctttgtgg
     1381 ttttggaaga cttcccgctg accctcaacg gcaaaattga tcgcaaggca ttgacaaccc
     1441 atcagggagg ccgatggcat caagtcactc gtcaggtgcc gccacgcacg ctggtcgaat
     1501 ttcagctact taccctgtgg caacacctct ttcatcgtca gtcaatcagt attgaggacg
     1561 acttttttca gctcggaggg cattccctga tggcaatacg gctggcagca gcgattgaaa
     1621 aagaattcgg ctgccggttg ccgctcgcca tattgtttca ggaaccaacc attgcccggc
     1681 tggcagcagc ggtgcgcacg atgagccctg atcgggaatg gtcaccactg gtcacccttc
     1741 aacccagggg gt
//
LOCUS       DAOQDO010001552         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72147_length_1752_cov_2.633471, whole genome shotgun sequence.
ACCESSION   DAOQDO010001552 DAOQDO010000000
VERSION     DAOQDO010001552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72147_length_1752_cov_2.633471"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKZ53_20635"
     CDS             complement(<1..946)
                     /locus_tag="PKZ53_20635"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNJ42902.1"
                     /translation="MALTTYLEAIRQGIFEEMRRDPNVFCLGEDVAVYGGAFKVTAGL
                     LEEFGPDRMIDTPIAEAAIVGAACGAALMGMRPIAEMQFIDFISCGFDMLTNYAATSR
                     YRQGLGIPIVVRGPCGAGVRGGPFHSLNPEAFFMNTPGLKMVAPATAYDARGLIKAAI
                     RDDDPVLFFEHKFLYRRIKEDLPEAEYVVPIGKALTRREGEDLSIITYGSMVHTALEA
                     AHILQAEDDLEVEVVDLRSLLPYDKEAILATVKKTSKVILLHEATLTGGIAGEFAAFI
                     AEHAFEYLDGPIVRIGSIDTPTPYSPPLEDFFLPNVPKV"
     gene            complement(1066..>1752)
                     /locus_tag="PKZ53_20640"
     CDS             complement(1066..>1752)
                     /locus_tag="PKZ53_20640"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ42903.1"
                     /translation="QTNFSGANLAGADFEKADVLEANFDLAWLTDAQFVGTRLEKVSL
                     VGAIIKGANFEKSKLNANMTGMDLRVAKFAGATFGGSNLSQTDMRRMSLPGVSFKNSR
                     LVDARMAEMNLENTSFEDAELTGASMRGVNLNNANLNGSTMALADLTAASLLNARLQE
                     VKLERADLRFAKLNKANLERADLTRCNLTGTDFTDANLANTNFWAAKVKNAKLDAGLK
                     WKLLFLLFRY"
BASE COUNT          426 a          466 c          421 g          439 t
ORIGIN      
        1 ccacttttgg aacattcggt aaaaagaaat cttccaatgg ggggctatag ggcgttggcg
       61 tatcaattga cccaatgcgg acaatcgggc cgtcaagata ttcaaaggca tgttcggcaa
      121 taaaggcagc aaattcacca gcaatcccac cggtcaaggt ggcctcgtgt aacaaaatca
      181 ctttgctggt ctttttcact gttgccagga tggcctcttt gtcatagggc aataatgatc
      241 ggagatcaac cacttcaact tccagatcat cttctgcttg cagaatgtgg gcggcttcaa
      301 gcgccgtatg gaccattgat ccataggtaa taattgacag atcttcgcct tcccgccggg
      361 tcagtgcttt tccaatcggc accacatatt ccgcttcggg cagatcctct ttgattcggc
      421 gatagagaaa cttgtgctca aaaaacagca ccggatcatc atctcgaatg gcggctttga
      481 tcagtcccct ggcatcgtaa gccgtggccg gtgccaccat tttcaaccct ggtgtattca
      541 taaaaaaggc ttccgggttg agcgaatgga aagggccgcc tcggacacca gccccacacg
      601 ggccacggac cacaatcggg attccaagtc cctggcgata gcgactggtc gccgcatagt
      661 tggtcagcat atcaaagccg cacgaaatga agtcaataaa ctgcatttca gcaatcgggc
      721 gcatgcccat cagtgcggca ccgcaggcag cgcctacgat ggcggcttcg gcaattggcg
      781 tgtcaatcat ccggtctgga ccaaattctt ccagcagtcc agccgtgacc ttgaatgccc
      841 cgccatacac cgcgacgtcc tccccaagac aaaacacgtt tgggtcgcgt cgcatttctt
      901 caaaaattcc ctgtcggatt gcttcaagat aagttgttag ggccataaaa tttagaggtt
      961 atgagtgctt ggtttttcgt tcctggttct tcgttctttg tccttggttc ttggttcttg
     1021 gaaagcattg gttcctaagc actaagcacc aagcacctcc tggttttagt atcgaaacag
     1081 cagaaacaac aatttccact tcaacccggc gtccaatttt gcatttttca ctttggcagc
     1141 ccaaaagttg gtgttagcaa gattggcatc ggtgaaatca gtcccagtca aattgcagcg
     1201 ggtcaaatcg gcccgttcca ggttggcttt attgagtttg gcaaagcgca ggtcggcgcg
     1261 ttcgagtttg acttcctgca gacgggcatt aaggagtgag gcagcggtca aatcagccag
     1321 ggccatggtg gatccattca gattggcgtt attgagattg accccgcgca ttgaagcacc
     1381 agttaattcg gcatcctcaa agctcgtatt ttccaggttc atctcggcca ttcgggcatc
     1441 caccagacgc gaatttttaa aactcactcc tggcaaactc atccgccgca tatcggtttg
     1501 cgacaaattg gacccaccaa aggtcgcacc ggcaaatttg gcaacgcgca aatccatacc
     1561 ggtcatattg gcattgagtt tgcttttttc aaaattggcg cctttgataa tcgcccccac
     1621 caggctgacc ttttccaacc gggttccgac aaactgggcg tcggtgagcc aggccagatc
     1681 aaaattggct tcgaggacgt cagctttctc aaaatcagcc cctgccagat tggcaccaga
     1741 aaaattggtt tg
//
LOCUS       DAOQDO010001553         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72225_length_1751_cov_2.845519, whole genome shotgun sequence.
ACCESSION   DAOQDO010001553 DAOQDO010000000
VERSION     DAOQDO010001553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72225_length_1751_cov_2.845519"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKZ53_20645"
     CDS             <1..214
                     /locus_tag="PKZ53_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ42904.1"
                     /translation="PHPKIEKARQRIVLQGDVASPINPPTGCRFHTRCPHVMPMCREA
                     EPPLQDLGNGHLAACFLHTGGPEAKG"
     gene            complement(346..1659)
                     /locus_tag="PKZ53_20650"
     CDS             complement(346..1659)
                     /locus_tag="PKZ53_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HNJ42905.1"
                     /translation="MSSLLLDMQDFSSAPPSHTDDYSIADTLPKLVTQALRAETSSIA
                     FVLKLGKALHQYGTPAHRLEDTLVDISNTLGLEGQFFSTPTSIFASFGDPEEQRTTLI
                     RVEPGEVNLEKLVLLDTLAEQVLDGTLTPSEGAQQIDEIVAARPRYGDFLTAICFGIS
                     SGAASRFLGGGWREILMATVIGMALGIFAWLAGRYPNLSRVFDPVSAIIASVLAIVAT
                     QVFAPISVYITTLGGLIVLVPGLTLTVAMNELAQRSLVAGTARLMSAILLLIEIGFGV
                     ALGTQIQRILPAVSMFQRPEPLPWWTEPLALLIAPLSLAVLFRARPKDMGWILLSALV
                     GFWGARTGAWMLGPELGVCLGALLVGLAGNLYNKYKKRPAAVLLVPGIMLLVPGSVGY
                     GSVAKFLEKDVVSGVEAAFRMILVAIALVTGLLLANTTLPPRKAL"
BASE COUNT          449 a          480 c          489 g          333 t
ORIGIN      
        1 acctcatccc aaaattgaaa aagcccggca acggattgtg ctccagggcg atgttgcgag
       61 cccgatcaac cctccgactg gatgccggtt tcatactcgc tgtccacacg tgatgccaat
      121 gtgccgcgaa gccgaaccac cgcttcagga tctgggcaat ggtcacctgg cagcatgttt
      181 tttgcataca ggcgggccag aggctaaggg ctgagggctt ggggctgaag aatatcgggc
      241 tgaagaatac tgggtggctg aagacacggg aattctgctt tgaacccgaa gtcttcagcc
      301 ccgagcttgc gaatcttcag ccccaagccc tcagcccgcg tattttcaca atgccttgcg
      361 tggcggcagg gtggtattgg ccaggagcag cccggtgacc agtgcaattg caaccagaat
      421 cattcggaac gcggcttcaa ctccggacac cacgtctttt tcgaggaatt tcgcgacgct
      481 tccatagccg acgctgccag gaaccagcaa cataatgccc ggaacgagca ataccgccgc
      541 cgggcgtttt ttatatttgt tgtaaagatt tccggctaat cccaccagca acgcgcccag
      601 acacacccca agctccggcc ccagcatcca ggcgccggtt cgtgcgcccc agaaccccac
      661 cagggctgag agcaaaatcc atcccatatc ttttggacgg gctcgaaaga ggacggccag
      721 tgaaagcggt gcgatgagca gggccagcgg ttcggtccac cacggaagcg gttccggacg
      781 ctgaaacatg gaaacggctg gcagaatgcg ttgaatttgg gttcccagtg ccacgccaaa
      841 cccaatttca atcaggagca gaatcgcact catcaaccgt gccgtgccag caaccagact
      901 ccgctgcgca agttcattca tcgccaccgt cagggtcaat ccggggacta acacaatcag
      961 cccgcccagg gtggtgatat agaccgagat cggagcaaaa acctgggtgg ccacaatcgc
     1021 cagcaccgaa gcgataatgg ctgaaaccgg atcaaacacg cgtgacagat tgggataccg
     1081 acccgccagc caggcaaaaa tcccgagtgc cattccaatt actgttgcca tcagaatttc
     1141 acgccagccg cctcccaaaa atctcgaagc cgcacctgaa gaaatcccaa agcagatcgc
     1201 cgtcaaaaaa tcaccatacc ggggccgggc ggctacgatt tcatcaattt gttgagcccc
     1261 ctcactcggc gttaaagtgc cgtctaaaac ctgctcagcc agggtgtcga gcagaacaag
     1321 tttttcgaga ttgacttcac cgggttcgac acgaatgagc gtggtgcgct gttcttcagg
     1381 gtcgccaaag gaagcgaaaa tgctggttgg agttgaaaaa aactgacctt ccaaaccaag
     1441 cgtgtttgag atatcaacga gtgtgtcttc caggcgatgg gccggggtac cgtattgatg
     1501 gagggctttt ccaagtttga gaacaaacgc gatactcgac gtctcagcgc gtagcgcctg
     1561 ggtcaccagt ttgggcaagg tatcggcgat agaataatcg tcggtatggc ttggcggcgc
     1621 tgaggaaaaa tcttgcatat caaggagaag tgaggacata gaggaaagag ggctaagggc
     1681 ttggggctga agaaaacggg ctgaagactc gcaagctcgg ggcggaagaa attgggctga
     1741 agaaattggg a
//
LOCUS       DAOQDO010001554         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72226_length_1751_cov_2.798349, whole genome shotgun sequence.
ACCESSION   DAOQDO010001554 DAOQDO010000000
VERSION     DAOQDO010001554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72226_length_1751_cov_2.798349"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKZ53_20655"
     CDS             <1..670
                     /locus_tag="PKZ53_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42906.1"
                     /translation="VTVAQAADTQSPTVTVNNPMEGAQFESRPNTQIQVSWQSTDDQG
                     VVSHSVELKGQRNGTAFQDMVATGLPGTASSFTLTIAENDAFLQGQVIVSATDAARNT
                     GMGQSGIFSVTAPPPPDTQSPAVSQVMLSKTSIKRKKDPSLTISWTSSDNVAVESHDV
                     LYATDGVTFDTTVASGLAGTLQQFTWTVPSTLPKTRQGRIKVTAKDQAGNQGEAMSES
                     LKIK"
     gene            complement(868..>1751)
                     /locus_tag="PKZ53_20660"
     CDS             complement(868..>1751)
                     /locus_tag="PKZ53_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ42907.1"
                     /translation="LLKGMADGKYPEMIHVFLDASFVTGHHVFADSVNNGPWGRALTE
                     EFIPYLEKNFRQLPKTAARFLTGHSSGGWSTLWLQITYPDFFGGTWSTSPDPVDFRSF
                     SGINASPGTTDNLYRAADGKPHPLVRIGGQVVFTIEAYTLKEEIETDGLGGQLSSFEW
                     VFSPKDRDGRPMKLFDRTTGEFDQRVLKAWQKYDIRLTLEKNWATLGPKLAGKIHIVC
                     GEADTFYLEGAVRLLRDFLREKGSDAVVELVPDRGHFDLYKPFETYKEGLETRIFHEM
                     ETAFQSASKPGKSKVKK"
BASE COUNT          461 a          436 c          431 g          423 t
ORIGIN      
        1 ggtgacagtg gcccaggccg ccgataccca atccccgacg gtgactgtga ataatccgat
       61 ggagggcgcc cagtttgaat cgcgtccaaa tacacaaatc caggtttcgt ggcagtcaac
      121 tgatgatcag ggcgtggtga gccattcggt tgaactcaaa gggcaacgaa atggaacggc
      181 gtttcaagat atggtggcaa ccggattgcc cgggacagct tcgagtttta ctctgacgat
      241 tgctgaaaat gatgcgttcc tgcaaggtca ggtaattgtt tccgccactg atgccgcccg
      301 aaatacagga atgggacaat ctggaatttt ctcagtcacg gccccacctc cacccgatac
      361 tcaatctcca gccgtgagcc aggttatgct gtcaaagaca agtatcaagc gcaaaaaaga
      421 cccatcgctt acgatttcct ggacgtcatc agacaatgtg gcagttgaaa gtcacgatgt
      481 tttatatgca accgatggtg ttacatttga caccactgtg gcttcaggtt tagctggtac
      541 actgcagcaa tttacctgga ctgtgccttc aacccttccc aaaaccaggc aaggtcggat
      601 caaagtcacg gcaaaagacc aggctggcaa ccagggagaa gccatgagtg aaagcttgaa
      661 gatcaaatag aatcgagaga aacgggaatg aagaattgag actggtgact ggtgactggg
      721 aaatatcttc cagtcaccag ttataccatt ttggaatgaa gctatcatat tagcaaaagg
      781 ctcaattatt gggataattg ggtttcactg acccctgacc ccgagcccct gatcccaaaa
      841 tggtatgacc aggtcccaaa aacaaagcta ttttttcact tttgattttc ctggtttgga
      901 ggctgattga aatgccgttt ccatctcgtg gaagattcga gtttcaagtc cttccttgta
      961 agtctcaaag ggtttgtaca ggtcgaaatg gccacgatct gggacaagtt caaccaccgc
     1021 gtcacttcct ttttccctca gaaaatcccg caggagtcgg acagcgcctt cgagatagaa
     1081 ggtatccgct tcgccacaaa caatgtgaat tttcccggcc agtttcgggc caagcgttgc
     1141 ccagtttttc tccagcgtga gacgaatgtc gtatttctgc caggctttca gcactcgttg
     1201 gtcaaattca ccggttgtcc gatcaaacaa tttcatcggc ctcccgtctc tgtctttcgg
     1261 gctaaatacc cattcaaatg aagagagttg cccgcccaga ccatcagttt caatttcttc
     1321 tttaagcgtg tatgcctcaa ttgtaaacac aacttgccca cctatacgca cgagcggatg
     1381 aggtttcccg tcagcagccc ggtataaatt atccgttgta cccggcgagg cattgatgcc
     1441 tgaaaagctg cgaaaatcca ctggatcagg tgacgttgac caggtgccac cgaaaaagtc
     1501 agggtaggtg atttgaagcc acagcgtgct ccagccacca gaggaatgac ccgtcaggaa
     1561 gcgagcggcg gttttgggaa gttgacgaaa gtttttctca aggtagggga tgaattcctc
     1621 agtgagtgcg cgtccccagg ggccgttatt gacggagtcc gcaaaaacat gatggcctgt
     1681 tacaaacgaa gcatccagaa aaacatggat catttcgggg tacttcccgt cggccattcc
     1741 cttgagcaac t
//
LOCUS       DAOQDO010001555         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72254_length_1751_cov_2.220519, whole genome shotgun sequence.
ACCESSION   DAOQDO010001555 DAOQDO010000000
VERSION     DAOQDO010001555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72254_length_1751_cov_2.220519"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKZ53_20665"
     CDS             <1..305
                     /locus_tag="PKZ53_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42908.1"
                     /translation="VFGTVSDQPDFAANIEDVIFIPTPHPVTDLRVADDGQVWFCGPA
                     GAQGYVGRVDTSLTLHTESETLPGQANAINLTSTGYSVVGKVPQTTGSEAFVRYEP"
     gene            794..>1751
                     /locus_tag="PKZ53_20670"
     CDS             794..>1751
                     /locus_tag="PKZ53_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42909.1"
                     /translation="MKRTDVIGIRDLLRCTCLLAVSLPVIFPFANPVFSQSAPGFTNQ
                     GIGNEANVTLALANQIITAGQTTSSTLPNSGQPQNTNGGLSDGYIAVYSTGGTIAWST
                     FLGGSQSDAIRDAVVASSNVLILTGVTESSDFPVTDGSTLQGPSGAFVAAINLETQSL
                     IYSSILPGSSREIGHKLVQLADGRIAVVGERTTNGVTTGFVAVQPPLGETGSRLVLEL
                     DGPVFAVAQNTNGNLIVVGGYDTGLGFIKGVNPTTLEITSALDLPMVGRTVTVASNGK
                     IWVGGSNASVSNRPSFHVQPLDGDNTGQLGQLGRPPVTRPVFG"
BASE COUNT          411 a          431 c          416 g          493 t
ORIGIN      
        1 cagtctttgg aaccgtctct gaccaacctg attttgccgc caatatcgaa gacgtgatct
       61 ttatccccac gccacaccca gtgactgatt tacgggtggc cgatgatggg caggtctggt
      121 tttgcggtcc ggctggggcc cagggctatg tcggacgggt tgatacatcg ttgacgcttc
      181 acactgaatc ggaaacgctt cctggccagg ccaacgcaat caatctgact tcaaccggtt
      241 attcagtcgt cgggaaagtc ccccaaacga ctggttcaga agcctttgtg cgctacgaac
      301 cataacttga ttcttggtgt ttgctttctg gttcttttat tgtggaaagg tcgaggtgac
      361 ctttccacaa tgaaatctct acaaagttga cgctgaatcg aaagtattgt ccccactacc
      421 ctctccttct ccactttgct cccgtcaggt tccattctca tctgtttatt ttcccttgtt
      481 cttctgttca ggcaaaattt tggcgctttt gttgagttga tcttgaaaat acttcacaag
      541 tgtttcaaaa atgatttgca gttctcaaaa accgtgagct agctggtctc agaagattgg
      601 gaaatttgaa ttgaatttcc tgatttccct ctctgattag tttggaaaaa ctgaacttcc
      661 catgagttca aatttctttg cggttaaatg ttgagttgac tgtgaaacga attgatcttc
      721 aactcatctc tggtcagtcc agagaattga atcagactca gcgcagaatg aagtctaagt
      781 ggagaatgcc aatatgaaac gaactgacgt gataggtatt cgtgacctgt tgagatgtac
      841 ctgtttactg gctgtaagcc tgccagtgat tttccctttt gctaacccgg tcttttcaca
      901 atcggcgccg ggctttacca atcaagggat tgggaatgaa gccaatgtga cgttggcgct
      961 tgcgaaccag atcattaccg ctggccaaac aacctcatca acccttccca atagtgggca
     1021 accacaaaat acaaatggcg gtttatctga tggatacatc gcggtttata gcactggcgg
     1081 tacaattgcc tggtcaacct tccttggtgg aagtcaatca gatgcgattc gggatgcagt
     1141 tgtagcatca tcaaatgtgc ttatcctgac tggagttacc gagtcttctg attttcccgt
     1201 caccgatggc tcaaccctgc aaggcccatc aggtgctttt gtcgccgcca tcaacctgga
     1261 gacccagtcg ttgatctatt cctcaattct tccaggttcc agccgtgaga ttggtcacaa
     1321 actggttcaa cttgctgatg gccggattgc cgttgtcggc gagcgtacca ccaatggtgt
     1381 caccaccgga tttgttgccg tccaaccgcc acttggcgag actggaagcc ggcttgtgct
     1441 tgaactggat gggcctgtgt ttgcggttgc ccaaaatacc aatggcaatc tgatcgttgt
     1501 cggtggctat gacaccggcc ttgggtttat caagggtgtt aatcccacca cgcttgaaat
     1561 tacctcggcg cttgaccttc ccatggttgg gagaaccgtg acagtcgcaa gcaatggaaa
     1621 aatctgggtc ggagggagca acgcatcagt ttccaatcga ccgtcctttc atgttcaacc
     1681 actggatggt gacaatactg gacagctcgg acaattaggg agaccaccag tcacaagacc
     1741 agtttttgga a
//
LOCUS       DAOQDO010001556         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72380_length_1749_cov_2.701299, whole genome shotgun sequence.
ACCESSION   DAOQDO010001556 DAOQDO010000000
VERSION     DAOQDO010001556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72380_length_1749_cov_2.701299"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKZ53_20675"
     CDS             <1..574
                     /locus_tag="PKZ53_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNJ42910.1"
                     /translation="FSIASRYGTPPMTVAHGGGVFTTRTMIRGDADEQALLAKNREVM
                     AALGLVHGVSHTEFIKGAEDGQFYFLETASRVGGANIVELIEAATGMNLWAEWAKLEI
                     SDDKNPYKLPKMKKNHAGLLVSLARQEYPDYLAFKDPEIVWTLHKKHHAGLIVSSPDY
                     HRIDELLADYVERFTRDFTAFQPSPERPTG"
     gene            816..>1749
                     /locus_tag="PKZ53_20680"
     CDS             816..>1749
                     /locus_tag="PKZ53_20680"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42911.1"
                     /translation="MTNHPDKVLVVDDDPNFRLIVRKVLTKAGFTVTQADDGQKGLDA
                     AEHDQPGVILLDWMMPILDGPSVCRRLRENPEFQNLQIIMVSAKGELDDKISGLDSGA
                     DDYLVKPCEPKELLARVRSAMRIYELKRQLADKANWEALLRRVADRIRSSLNIEEILR
                     ATVEEIRVVARADRAVIFELDVASHQMLIRTEAIGPEISPSHHLVLEVSTAQFFYSVL
                     DQNKSNQISDIQTATGIEDLRPWIQQTQVRSLVAVPILLSEKGQTTTLYGLALHQCRE
                     SRTWSEEEIPFLESTAPQPGIALDHARLYAQAQQQ"
BASE COUNT          431 a          436 c          458 g          424 t
ORIGIN      
        1 gttttcaatt gccagtcgct atggaacccc tccgatgacg gtggcccatg gcggcggggt
       61 ttttacaacc cggactatga ttcggggaga tgctgacgaa caggcactgc tggccaaaaa
      121 ccgggaagtg atggccgcgc ttggtcttgt ccacggggtt tcgcacactg aatttatcaa
      181 aggtgcggaa gatggtcagt tttacttcct cgaaacagca tcgcgggtgg gtggcgccaa
      241 tattgtggag ttgattgaag cggccactgg catgaacttg tgggcagagt gggcaaagct
      301 cgaaatcagt gatgacaaaa acccgtataa gctccccaaa atgaaaaaga accacgctgg
      361 cctcctggtt tcgcttgccc ggcaggaata cccggattat ctggccttta aagatcctga
      421 aattgtctgg accctccaca aaaaacatca tgctggcttg attgtatcct cgccagatta
      481 tcatcgaatt gatgaattac tggccgacta tgtcgaacgc tttacgcgtg atttcaccgc
      541 gtttcagccg tcgcctgagc gaccaaccgg ctagagtcca gtcccctggt caggatcggt
      601 tcaactggtt gtaaggtgta caggttggga aaccgggacc tgcaggttca gacttcaaag
      661 ctgtttggaa atttttggaa atgtggtcag gcaggccaca tttccaacca gtttttgagt
      721 gccccttttt tgcattctgg aatctttcgt ccagattaaa cgggtttccc cgctttgatc
      781 acacgaaatt ctttgatggg gtgagaggtg ttggtgtgac aaaccatccg gacaaagtgc
      841 ttgtggttga tgatgacccc aattttcgtc ttatcgtgcg taaagtactg accaaggctg
      901 ggtttacagt gactcaggca gatgatggac aaaaagggct ggacgcagcc gaacatgatc
      961 aacctggggt gattctcctg gactggatga tgccaatcct ggatggacca tctgtgtgcc
     1021 gtcgtctgcg tgaaaatcct gaatttcaaa atctgcaaat catcatggtc tcggccaaag
     1081 gcgaactcga cgataagatc tctggccttg attcaggggc cgacgactat ctggtcaaac
     1141 cctgtgaacc caaagaactc ctggctcgcg ttcgaagcgc catgcgcatt tatgaattaa
     1201 agcgccaact ggcagacaaa gcgaactggg aagctctgct gcgccgggtg gcagaccgga
     1261 tccgaagctc gctcaatatt gaagaaatcc tgcgcgcaac ggttgaggaa atccgggttg
     1321 tggcccgggc tgaccgggcc gtgatttttg aactggatgt ggcgagtcat cagatgctga
     1381 ttcgcaccga agccattggg cctgaaattt ccccaagtca ccatctggtc ctcgaagtat
     1441 ctacagctca atttttctat tcggttttag accaaaataa atcaaatcag atttctgata
     1501 ttcaaacggc aactgggatt gaggatctca ggccctggat tcaacaaacc caggtgcgat
     1561 ctctggtcgc cgtcccaatt cttttgagcg aaaaaggaca aaccacaacg ctctatggac
     1621 ttgccctgca tcaatgccgt gaatcgcgga cgtggtcaga agaagaaatc ccttttttgg
     1681 aatcaacggc cccccaaccc ggcattgcac ttgaccatgc ccgcctctat gcccaggcgc
     1741 aacagcagg
//
LOCUS       DAOQDO010001557         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72571_length_1747_cov_2.148345, whole genome shotgun sequence.
ACCESSION   DAOQDO010001557 DAOQDO010000000
VERSION     DAOQDO010001557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72571_length_1747_cov_2.148345"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKZ53_20685"
     CDS             complement(<1..826)
                     /locus_tag="PKZ53_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42912.1"
                     /translation="MRWQPEFFQPVAGKFRVFVTLALTAVAVCLTDHPCQAQVSGSEA
                     TLPYTLQTRGATVLQEDFRMIGTPIPLAQSAGVAARGHAVPGNGAAPEVKVLESIRTL
                     KVGESQILTFTFDDRENDPLFAGVVGFLPQIVQDGRFFSKTKIKPDRSQLGRTIDIEV
                     LPSPAAGTIVWRLTALRPGTAVLLLVATELFASTDAGQFQLFAGNVSFEPVTLTVTDA
                     LTLPPVPALDTLPPDFVLRRGEALTWVTTSRAPSARPLTYGFGVLGFAGRIVEAKFT"
     gene            complement(909..1580)
                     /gene="gph"
                     /locus_tag="PKZ53_20690"
     CDS             complement(909..1580)
                     /gene="gph"
                     /locus_tag="PKZ53_20690"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNJ42913.1"
                     /translation="MPTPRQVGIMLFDLDGTLVDSRQDLTIALNEALDQLHLPPLTIE
                     TVSKFIGDGARKLVERGLHAGLGRDATPEETKQGLVLFKEAYGRHLTDSTLPYPNVVE
                     MLRHFQDLPKGVVTNKPYQFTVDLLEALEMARFFSVVIGGDTLPERKPRPEPVLAALT
                     RCQIPVSQAVMVGDSANDILAGRAAGVYTCGVTYGLRPRSELEQAGADVIVDEVLDLR
                     NLFVA"
BASE COUNT          462 a          465 c          467 g          353 t
ORIGIN      
        1 cggtgaattt tgcttctaca atccgaccag caaacccaag gactccgaag ccataggtca
       61 atggtcgagc cgatggtgcc cggctggtgg tgacccaggt tagtgcttca ccacgacgga
      121 gaacaaaatc aggtggcaat gtatccagtg ctgggactgg tggtaacgtg agcgcatcgg
      181 tcactgtcag cgtgactggt tcaaaactga catttccagc aaagagttga aactgaccag
      241 cgtcggtact ggcaaacaat tcagttgcta cgaggagcaa aactgcggtt ccaggtcgca
      301 aggcggtcaa acgccagaca attgttcctg ctgccgggct gggcagtact tcgatatcaa
      361 tcgtgcggcc caactggctt cgatctggct tgattttcgt ttttgagaaa aagcgcccat
      421 cctgaacaat ttgcggtagg aacccaacca cgccggcaaa cagcggatca ttttcacgat
      481 catcaaaggt aaaggtcaga atctggcttt caccgacttt gagcgtacga atagattcaa
      541 gtactttgac ttcaggagcg gccccattgc ctgggacggc atgaccacgg gcggccaccc
      601 cggcggattg ggcgagcgga atcggcgtgc cgatcatccg gaaatcttcc tggagaaccg
      661 tggcgccacg tgtttgcaag gtatagggca gtgtcgcttc tgatccgctg acttgagcct
      721 ggcaggggtg atccgtcagg cacaccgcca ctgccgtcag agccagcgtg acaaaaacgc
      781 gaaacttccc cgcaacaggt tggaaaaact caggctgcca tctcatagtc aatcagggga
      841 tccaaaaacg ggataaaaag aggtgctatt cttaccatat ccccgtggga ctggacaaaa
      901 attttcgctt acgccacaaa caaattccgt aagtccagca cttcatccac aatcacatcc
      961 gcgccagcct gttcaagttc actccgagga cgcaatccat acgtcacacc acaggtgtaa
     1021 acgccagccg cacgcccagc cagaatatca ttggcactgt cgccaaccat cacggcttgc
     1081 gagaccggga tttgacaccg tgtcagggcg gccagtaccg gttccgggcg cggcttgcgt
     1141 tcgggcaagg tatcgccgcc gatcacaacc gaaaagaacc gggccatttc aagcgcttcg
     1201 agtaagtcaa cggtgaattg atagggttta ttggtgacca cgcctttcgg aagatcctgg
     1261 aaatggcgca gcatttcaac gacattcggg tagggaagcg ttgaatcagt taaatgccgc
     1321 ccataggctt ctttaaatag aaccaggccc tgtttggttt cttcgggagt cgcatcgcgg
     1381 ccaagcccgg cgtgcaggcc gcgttcaacc agtttcctgg cgccatcgcc aataaattta
     1441 ctgacggttt caatcgtcag gggcggcagg tgaagctggt cgagtgcttc attaagtgca
     1501 atggtgagat cctggcggga atcaaccagt gtgccatcta agtcaaacaa catgattccc
     1561 acctggcgag gtgtcggcat ggaaaagctc ctttgattga gaaagtccct gagattggga
     1621 aagtgaaaaa ggacgaaaag gacgaaagag atgaaaagga cgaaaaagac aaaaaggaca
     1681 aaaaggacct aacttcgaaa accccgaacc ccgaaccctg aaccccaacc ccggagactt
     1741 tcccatg
//
LOCUS       DAOQDO010001558         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72599_length_1747_cov_1.329196, whole genome shotgun sequence.
ACCESSION   DAOQDO010001558 DAOQDO010000000
VERSION     DAOQDO010001558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72599_length_1747_cov_1.329196"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKZ53_20695"
     CDS             <1..337
                     /locus_tag="PKZ53_20695"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNJ42914.1"
                     /translation="FDHKYGEDVWGYYAKTPDRAAIFNESMTNVSENTNAAVVAAYDF
                     SSIRKLVDVAGGHGSLLAGILSANPHLHGVLFDQPHVVNGANAKLSDFADRCEIVANG
                     NPAGNCHRN"
     gene            276..>1747
                     /locus_tag="PKZ53_20700"
     CDS             276..>1747
                     /locus_tag="PKZ53_20700"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42915.1"
                     /translation="MQIVVKSSPTATQQVTATGTEASDAVEKPVTVQPDGEEQIQTDG
                     QLFSDSVKFETLVPTTVLSGTLRSTLKLYPNLLGHVFEGIEGILQRPHGCAEQTISST
                     YPNVMVLKYLAQLEQPEAVTTQRARDYTQQGIDRLLGYQHETGGFTYWGNGSPDLTLT
                     AYAIRFLTDAQEFATVDESVIDWARRYVLSQQQTDGSWAFDNRYSSPVQSVYDLQQSA
                     YVALSLASSGEKSATFQKALDRLASQIATSDSPYWLAVTALAASRAGQLDLAAQAVAR
                     LGKLIKPGTEQVSWNQSLETPFHGWGRAAEVETTALALRAILAVTGNVQASDWQSRFN
                     RGLLYLLQNKDQHGVWLSTQASVAVWELLFALNEQEREARKMASALEVWVNGQPFKTI
                     DLTGVENRLSPITVDLTERFTSGRNQVEVRSQQPVALMSAQLMTRLYAPWTVEPGKVA
                     HQSPDLNFNVNFDKIEATLSDVVTCRVDYERKDRGNGMLLA"
BASE COUNT          424 a          442 c          491 g          390 t
ORIGIN      
        1 attcgatcat aaatatggcg aagatgtctg gggatattac gctaagacac cggatcgcgc
       61 ggcgatcttc aatgaatcca tgacgaacgt cagcgaaaat accaacgcgg cagtcgtggc
      121 cgcgtatgat ttctcgtcca tccgaaaact ggtggacgtg gcaggcggcc acggcagctt
      181 actcgcgggc attctcagcg cgaatccgca tttgcacggt gtgttatttg accaaccgca
      241 cgtcgtgaat ggcgcaaatg ccaagctcag cgattttgca gatcgttgtg aaatcgtcgc
      301 caacggcaac ccagcaggta actgccaccg gaactgaagc cagtgacgcg gttgaaaaac
      361 cagttactgt ccagccagat ggcgaggaac aaatccagac tgatggacag ctcttctctg
      421 acagcgtgaa gtttgaaact ctcgtcccga cgacagtttt gtctgggaca cttcgcagca
      481 cgttgaaact gtatccaaat ttgcttgggc acgtgtttga aggcattgaa ggcattttgc
      541 agcgtccgca tggctgtgcc gaacagacga tttcttcgac gtatccaaat gtgatggtgt
      601 tgaaatacct ggcacaactt gaacaaccag aagctgttac cactcaacgg gcacgcgatt
      661 acactcagca gggaattgac cggttgctgg ggtatcagca tgaaacaggt gggtttacct
      721 attgggggaa cggttcgccg gatttgacct tgacggccta tgccatccgg tttttgactg
      781 atgcgcagga atttgccacg gtggatgaat cagtaattga ctgggctcga cgctatgttt
      841 tgagtcagca acaaactgat ggaagctggg cgtttgacaa ccggtattcc agcccggttc
      901 aatcggtgta tgaccttcag caatcagcct atgtggcgtt aagtctggcc agttccggag
      961 aaaaatctgc aaccttccag aaggcccttg accgactggc cagccagatt gcaacgagtg
     1021 atagcccata ctggctggca gtaacagcgc tggcggccag tcgcgccggt caactcgatc
     1081 tggccgctca agccgtggcg cgattgggca aactgatcaa accgggcacc gaacaagttt
     1141 cctggaatca atcgcttgaa acgccatttc acggttgggg tcgcgccgca gaggttgaaa
     1201 ctacggcgct ggcgctacgg gcaattctcg cggtgacagg aaacgttcag gcatcggact
     1261 ggcaatcgcg gttcaatcgg ggattgctct atctgcttca gaataaagat cagcacggcg
     1321 tctggctttc aactcaggct tcggttgccg tgtgggaatt gttgtttgca ctgaacgagc
     1381 aggagcgcga agccagaaag atggcttccg cgctcgaagt ctgggtcaat ggtcagccgt
     1441 tcaaaacgat tgacttgacc ggagtggaaa accgtttgtc tccgatcacg gttgacctga
     1501 cagagcgctt tacatcgggc cgcaatcagg tggaagtccg ctctcagcaa ccagttgcgt
     1561 tgatgagtgc gcaactgatg acccgcctgt atgcgccctg gaccgtggaa ccgggaaaag
     1621 tcgcccatca atctccagat ttaaacttca atgtaaattt tgacaaaatc gaagccaccc
     1681 tgagcgacgt ggtgacgtgc cgggttgact atgaacgcaa agaccgtgga aatggcatgc
     1741 tgctggc
//
LOCUS       DAOQDO010001559         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72625_length_1746_cov_2.943820, whole genome shotgun sequence.
ACCESSION   DAOQDO010001559 DAOQDO010000000
VERSION     DAOQDO010001559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72625_length_1746_cov_2.943820"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1746
                     /gene="acs"
                     /locus_tag="PKZ53_20705"
     CDS             <1..>1746
                     /gene="acs"
                     /locus_tag="PKZ53_20705"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNJ42916.1"
                     /translation="KEDRFFSPSTEFSAQAHIGSKEAYDQLVAEGERDPEGFWARAAE
                     ELHWFRKWDTVLKWEYPHAGWFLGGKLNVAYNCLDRHLSGPRRNKAAIIWEGEPGEIR
                     TLTYQQLYTEVCRFANVLKRLGVKKGDRVAIYMPMMPEAAVAMLACARIGAPHTVVFA
                     GFSADALRDRINNCGCVAVITADGAFRRGNEVALKPAVDRAIDECPSVQNVVVLKRTG
                     TKIHMEPGRDHWWHELIPTVSNICPAEELDSEHPLYILYTSGTTGKPKGILHTTGGYL
                     TQVALTSKIVFDLKETDTYWCSADIGWVTGHSYVVYGPLANGATVFMYEGAPNFPQPD
                     RFWEIIDRHQITIFYTAPTAIRAFIKWGEQWTFKHDLNSLRLLGTVGEPINPEAWMWY
                     SKVIGKGRCPIVDTWWQTETGAILITPIPGVTATMPGTATRPFPGLSIEIRTKDNKPV
                     GPNEGGYLVITKPWPSMLRTLWGDDERYVASYWSEIPGVYFAGDGARRDSHGNFWIMG
                     RVDDVVNVSGHRLGTAEIESALVSHPAVAEAAVVGRPDELKGQAIAAFVTLEGTFSAS
                     EELKKSLPPSADYPKL"
BASE COUNT          402 a          479 c          480 g          385 t
ORIGIN      
        1 aaagaagatc gttttttttc cccctcaacc gaattttcag cccaggccca catcgggtca
       61 aaagaagcct atgaccagct cgtggctgaa ggagaacggg atccggaagg attctgggcc
      121 cgggcggctg aagagttaca ctggtttcgc aagtgggaca ccgttctcaa atgggaatat
      181 ccgcacgccg ggtggttttt gggcggcaaa ttgaatgtgg cctataactg ccttgaccgg
      241 catctttctg gcccacgccg caacaaggcc gccattatct gggaaggcga gccgggcgag
      301 atcagaaccc ttacttatca gcagctttat accgaagtct gtcgctttgc caacgtgctc
      361 aaacgcctgg gcgtgaaaaa gggcgaccgg gtggcgattt acatgccgat gatgcccgaa
      421 gctgccgtcg ccatgctggc ctgtgcccgg attggcgctc cgcatacggt tgtgtttgcc
      481 ggattttccg ccgacgcgct ccgtgaccga atcaataact gtggctgcgt ggcggtgatt
      541 acggcagatg gcgcctttcg gcgtggcaat gaagtcgcgc tcaaacccgc cgttgatcga
      601 gccattgatg aatgcccatc cgttcaaaac gtcgttgtcc tcaaacgaac cggcacaaaa
      661 atccatatgg aaccaggacg cgaccactgg tggcacgaat taatcccaac ggtttcaaac
      721 atttgtccgg ctgaagaact cgacagcgag catccactct atattttgta cacttcgggc
      781 acgaccggaa aaccaaaagg aatcctgcac accacaggcg gctatctgac tcaggtagcg
      841 ttgacctcaa agattgtctt tgacctcaaa gaaactgata cctactggtg ttcggcggat
      901 attggctggg ttaccgggca cagctatgtg gtctatggtc cactcgccaa cggtgccacc
      961 gtgtttatgt atgaaggcgc ccccaacttt ccacagccgg atcggttttg ggaaattatt
     1021 gaccggcatc aaattacgat tttctacact gcgccaacag ccattcgggc ctttatcaaa
     1081 tggggtgagc agtggacctt taagcacgac ttgaattcat tgcggttgct gggcaccgtc
     1141 ggtgagccga tcaatcccga agcctggatg tggtattcaa aagtcatcgg caaaggtcgg
     1201 tgtccgattg tggatacctg gtggcagacc gaaaccggcg ccattttgat cacccccatc
     1261 ccaggcgtga cggcgaccat gccaggaacg gcaaccagac catttccagg tctgagcatt
     1321 gaaatccgaa ccaaagacaa caaaccggtc gggccaaatg aaggcggcta tctcgtcatt
     1381 accaaaccct ggccgtccat gctgcgcacg ctgtggggcg atgatgagcg gtatgtcgct
     1441 tcctactgga gtgaaatccc cggtgtgtac tttgccggag acggcgcccg tcgtgacagc
     1501 cacggcaatt tctggattat gggccgcgtg gatgatgtcg tgaatgtcag cggtcatcgc
     1561 ctgggcacgg ctgaaattga aagcgcgctg gtttcacacc cagccgttgc ggaagccgcc
     1621 gtcgtgggcc gaccagatga actcaaaggg caggcgattg cggcctttgt taccctcgaa
     1681 ggcacatttt cggcttcgga agaattgaaa aaatcgcttc ctccctccgc ggattatcca
     1741 aaactc
//
LOCUS       DAOQDO010001560         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72626_length_1746_cov_2.931993, whole genome shotgun sequence.
ACCESSION   DAOQDO010001560 DAOQDO010000000
VERSION     DAOQDO010001560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72626_length_1746_cov_2.931993"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="hpnK"
                     /locus_tag="PKZ53_20710"
     CDS             <1..757
                     /gene="hpnK"
                     /locus_tag="PKZ53_20710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HNJ42917.1"
                     /translation="MVTGEACTQAVVSAKARPRLSVGLHLVLICGRGALPPSEIPHLV
                     DPNGNFSNDPFTAGVKYYFSKAARAELRREIWAQFELFAATGLPFAHVDGHLHLHMHP
                     VAFSYLLEAARHFGVKRIRLPREPLFSNLKIRRSHVGQKLLHWSVFHLLSRSALKKMK
                     HDRFLLSDRVYGLLETGRMTEEFWLALLPQLTDALNEIYCHPEVAEPGADPENTGGRQ
                     ELAALMSPQIRALLPKYNIQLELNEPSLIQQQH"
     gene            778..1206
                     /locus_tag="PKZ53_20715"
     CDS             778..1206
                     /locus_tag="PKZ53_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNJ42918.1"
                     /translation="MRFLPILLISILVNAFGNTLLSRAMKEIHDPILAAFHAGQYGAA
                     VLVVLLNPTFWIAVGLLTAFFLLFLTMLSKADLSYVLPLTAINYVITVFLAAWFLGEH
                     VTVTRWIAVTFISMGVLMVSVGESSTVESQELSIPAEEQA"
     gene            1203..1583
                     /locus_tag="PKZ53_20720"
     CDS             1203..1583
                     /locus_tag="PKZ53_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNJ42919.1"
                     /translation="MITITLIAVVVLAGSVGDIFLSRGMKQVGEINSLHWRFLLQVSQ
                     RALTNLSLIGGIGGMAVAFFSWLAVLSFADLSFAEPATAVSYVLNTLGAKFFLNERID
                     RMRWAGTLLVCIGAALLSLDPGIK"
BASE COUNT          342 a          454 c          475 g          475 t
ORIGIN      
        1 gatggtgaca ggtgaggcat gtacccaggc ggtggtatca gccaaagccc gaccacgcct
       61 ttcagttggg ctgcacctgg tgttgatttg tggtcgtggg gcgctccctc catcggaaat
      121 tccgcatctg gttgacccaa acgggaattt ttcaaatgac ccgttcaccg ctggggtgaa
      181 gtattatttc tcaaaagctg cccgggcgga attacgccgt gaaatttggg cgcaatttga
      241 gctatttgcc gccaccggtc tccccttcgc acacgttgat ggccacctgc atctgcatat
      301 gcatccggtt gctttttcat acctcctcga agccgcccgg cactttggcg tgaagcggat
      361 ccgtcttcca cgcgaaccac tgttttccaa tctcaaaatt cgtcggtcac acgttggtca
      421 aaagctttta cactggagtg tgtttcacct tctctcacgc tcagcactca aaaaaatgaa
      481 gcacgaccgg tttttgcttt cagatcgggt gtatggactg ctggagacag gtcggatgac
      541 cgaggagttc tggctggcac tccttcccca actgacagat gccttgaatg aaatatattg
      601 tcatcctgaa gttgccgaac ctggtgcgga tccggaaaat actgggggca gacaggaact
      661 ggccgccttg atgagtccgc aaattcgtgc tttgcttccg aaatacaaca tccagcttga
      721 gttgaacgaa ccctctttga tccagcagca acattgaatc gctgcattcc aaagtctatg
      781 cgttttcttc cgatcctgct tatttcaatc ctggtcaatg cgtttggaaa cacgctgctc
      841 agtcgggcca tgaaagaaat tcatgacccg attctggcgg cgtttcacgc cgggcaatat
      901 ggggcggcgg tcctggtcgt actcctgaac ccaactttct ggattgcggt tggtttgctg
      961 acagcctttt tcctcctgtt tttgaccatg ttgtcaaaag cagacctgag ctatgtttta
     1021 cctctcacgg ccatcaacta tgtcatcaca gtgtttctcg ccgcctggtt tctaggcgag
     1081 catgtaacgg taacccgctg gattgccgtc acgttcattt caatgggagt actgatggtc
     1141 tcagttggag aaagctcaac cgtcgaatct caggaactat caatcccggc ggaggaacag
     1201 gcatgatcac cataactttg attgccgtcg tggtgttggc aggttcggtt ggcgacattt
     1261 ttctcagtcg tgggatgaaa caggtgggtg aaatcaattc cttgcactgg cggtttttgc
     1321 tccaggtatc ccaacgggca ttgaccaatc tgtcattgat tggaggcatc ggaggaatgg
     1381 cggtggcgtt tttttcctgg ctggcggtcc tgtcattcgc cgacttaagc tttgcggaac
     1441 ccgccaccgc cgtgagttat gtcctcaaca ccctgggcgc caagtttttc ctcaatgagc
     1501 gcattgaccg gatgcgctgg gccgggacgc ttctggtctg tattggtgcc gcattgcttt
     1561 cgctcgaccc tggaatcaaa taaaacctgt gtggagtgcg gtggcttgac accgctttgt
     1621 ggtctgccgg cttgacggct gtggagtgcg gtggcttgac accgctttgt ggtctgccgg
     1681 cttgacggct gtggagtgcg gtggcttgac accgctttgt ggtctgccgg cttgacggct
     1741 gtggag
//
LOCUS       DAOQDO010001561         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72647_length_1746_cov_2.516854, whole genome shotgun sequence.
ACCESSION   DAOQDO010001561 DAOQDO010000000
VERSION     DAOQDO010001561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72647_length_1746_cov_2.516854"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1060)
                     /locus_tag="PKZ53_20725"
     CDS             complement(191..1060)
                     /locus_tag="PKZ53_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42920.1"
                     /translation="MMKRTIAILFHETDATRDLTSYMITGMADIWREDGHDVIFLFGV
                     TRYVPADLILVHVDLSVVPDKYLDFAHRYPIVLNGKVKDIRKSTISQNLVKPGDAYDG
                     KVIVKTDLNYAGVPERIRNSTWLSENYPRLARLTSFVRPARSNQLAGVESPSDYRIYN
                     TVREVPSEIFQNKQFVVEKFLPEFENGQYYIRLFQFLGDRMTCLRLCAETPIVNATSR
                     SEREIVEPHPDMIALRHRLKFDYGKFDYVIHNGQPVLLDANKTVGGAKKATGSIQQSN
                     RKYRAAGIYSYFR"
     gene            complement(1057..1443)
                     /locus_tag="PKZ53_20730"
     CDS             complement(1057..1443)
                     /locus_tag="PKZ53_20730"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HNJ42921.1"
                     /translation="MARKLCQTRQTFLKVGNPSADRSWAEPVGMVLEIVPERDPTALH
                     SGEEFPVRVLKNGTPLPDFPLGLVRAGQKHGIIQKTDAEGRIVFHLKSGGKWLIRGTE
                     LRPSTQPDLDWESDFTTLTFEVARKP"
     gene            complement(1398..>1746)
                     /locus_tag="PKZ53_20735"
     CDS             complement(1398..>1746)
                     /locus_tag="PKZ53_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42922.1"
                     /translation="QLDLTSGMAFPALDTAIKPERVDRAKFRLADQTLDISKISPAAH
                     SLRFQTELAQVGIATLWVELKPKALTLTPAKVEEYLKEIDASESVRNRWKTSAVPKQW
                     RESYVKHAKPSSR"
BASE COUNT          418 a          402 c          460 g          466 t
ORIGIN      
        1 acaaatcgcg ttgaatatgc aggagaggca tgtattcaag gttcatggta agtaccttga
       61 cgtcaatttc ctgagagtct gggttttggt aagtatttga tttttttcgt gtatttcgtg
      121 tatttcgtgg ttaaaaatgt cctgaaatct tgagtagaat actcatttca aggtgcgaga
      181 aacgataaag ctaccgaaaa taggagtaaa tcccggcagc tcgatacttt cggtttgatt
      241 gctggatcga accggttgcc tttttggcgc cgccaacggt tttgttggcg tcgaggagca
      301 ccggctggcc gttgtgaatc acatagtcaa atttgccata gtcaaactta aggcgatgcc
      361 gcagggcaat catgtcagga tgtggttcaa caatctcgcg ctcactgcga ctggtggcat
      421 tgacaatcgg cgtttcggcg cagaggcgaa ggcaggtcat ccgatcaccc agaaattgaa
      481 acagccggat gtaatactgg ccattttcaa attcaggaag aaatttctca accacaaact
      541 gtttattctg aaaaatttct gatggcacct cgcgcactgt gttgtaaatg cggtagtcac
      601 tgggtgattc cacaccggca agctggtttg agcgggcagg ccgcacaaat gaggtcagtc
      661 tggccagacg cgggtaattt tccgaaagcc aggtggagtt gcggattcgt tcaggaacac
      721 cggcataatt cagatcagtt ttgacaatca ctttgccatc gtacgcatca ccgggtttga
      781 ccaggttttg actgatggtt gatttgcgaa tgtctttgac cttgccgttg agcacaatcg
      841 ggtaacggtg cgcaaaatca aggtatttgt cggggacaac tgagagatca acatggacca
      901 ggatcaaatc ggcaggcaca tacctggtga cgccaaacag aaaaatcacg tcgtgaccgt
      961 cttcgcgcca gatgtcagcc atgcccgtga tcatatagct ggtcagatcc cgtgtggcat
     1021 cagtttcatg aaaaagaatg gcaatggttc ttttcatcat ggttttctgg caacttcaaa
     1081 agtgagtgtc gtgaagtcac tttcccagtc caagtcaggt tgggtggagg gtctcaattc
     1141 agttccacgt atcaaccatt tgccaccgct tttcaggtgg aaaacaatcc tgccttccgc
     1201 atcggttttt tgaatgatcc cgtgtttttg accggcacgc accagcccga gcggaaaatc
     1261 tggaagggga gtgccatttt ttaaaacgcg caccgggaac tcctcgccag aatggagcgc
     1321 ggttgggtca cgctcaggca caatttcaag aaccatcccg actggttctg cccacgaacg
     1381 atctgctgac ggatttccta ccttgaggaa ggtttggcgt gtttgacata gctttcgcgc
     1441 cattgttttg gcaccgcaga tgttttccag cggttcctga cgctttcgga cgcgtcaatt
     1501 tctttcaaat attcttcaac tttggctgga gtcagtgtca gtgctttggg tttgagttca
     1561 acccagagcg tggcgattcc aacctgcgcc agctcggttt gaaaacggag cgaatgtgcc
     1621 gcaggtgaaa ttttcgaaat atccagggtt tgatccgcca gtcgaaactt tgcccggtca
     1681 acacgttcgg gtttgatggc ggtatccagg gctggaaacg ccatgccgct ggtcaggtcc
     1741 aactgg
//
LOCUS       DAOQDO010001562         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72741_length_1745_cov_2.327219, whole genome shotgun sequence.
ACCESSION   DAOQDO010001562 DAOQDO010000000
VERSION     DAOQDO010001562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72741_length_1745_cov_2.327219"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKZ53_20740"
     CDS             <1..796
                     /locus_tag="PKZ53_20740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNJ42923.1"
                     /translation="NEADVKDKPAFVKQLPPLSAENIKSIDKLYQNRLRTLLAVDEMV
                     ERVVETLRRTGQLDNTYIFFTTDNGFHLGTHRFTFGKGTPYEDAIRFPLIVRGPGVIA
                     GRELPAMIATIDLAPTFAELAGVTAPEFVDGGSLVSFLGAEIPPLEDQQHNVLIEHWP
                     GKGEDDRPDEQFAYFEATHRVLPDRKSGRKKRAPQWAAIRTDRYLYVEYVTGERELYD
                     LQVDPYELQNIYAAAEPSLLRSLEANLNALRSCVGRGCREAQRLRL"
     gene            complement(786..1631)
                     /locus_tag="PKZ53_20745"
     CDS             complement(786..1631)
                     /locus_tag="PKZ53_20745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ42924.1"
                     /translation="MTDKPRTELSQPFDFTKPELASVFDELSFWSSRFGALLIDNLVI
                     RKHQRILDIGCATGFPLFELAHIHGPTCTLVGVDIWKAALDRARHKRDIYGLTNVELI
                     EADASQMPFPEASFDLIVSNLGINNFADPDAVMKECFRVAADRARIVLTTNLFGHMTE
                     VYQVFRETLVRINKPHYLPKLAEQEHHRLSIDRVTALLESNGFQITHLVQQKFHLRFA
                     DGTAMLHHSLVKFGFLEGWLSAVSPDDEPEVYCEWEEDLNQLAHHAGEINTTIPMLLV
                     EAIKA"
BASE COUNT          451 a          412 c          458 g          424 t
ORIGIN      
        1 taatgaagcg gatgtgaaag acaagccagc ttttgtgaaa caattgccac cgttgtcagc
       61 agaaaacatc aagtcaattg acaaactcta tcaaaaccgg ctgcggacat tacttgccgt
      121 tgatgaaatg gttgagcgcg tagtcgaaac tttgcggcga acgggtcaat tggacaatac
      181 ctatattttc ttcacgaccg acaatgggtt tcatctgggc actcatcggt ttacttttgg
      241 aaaaggaact ccctacgaag atgccatccg gtttccgctg atcgtgcgcg gccctggggt
      301 tattgctggt cgagagttgc cggcaatgat tgccaccatt gacctggcgc cgacttttgc
      361 tgagctggct ggggtcaccg cgcctgagtt tgttgatgga ggctcgctgg tcagcttttt
      421 gggagccgag attccacctc ttgaagatca acaacataat gtactcatcg aacactggcc
      481 tgggaaaggc gaggatgatc gacctgacga acaatttgcc tattttgaag caactcatag
      541 ggtgttacct gaccgaaaat caggtcgcaa aaaacgagcg ccacaatggg cggcaatccg
      601 aactgaccga tatctgtatg ttgaatatgt caccggcgaa cgtgaactct atgatcttca
      661 ggttgacccg tatgaactcc aaaatatcta cgccgccgcc gaaccttcgc tcctgagatc
      721 attagaggca aatctcaacg cgctcaggag ctgtgttgga agagggtgtc gcgaggcaca
      781 acggctcagg ctttgatcgc ttcgaccagc agcatgggaa tggtcgtgtt gatttccccg
      841 gcgtggtgtg ccagttgatt taaatcttct tcccactcgc aatacacttc aggttcatca
      901 tccggggaaa cggctgacaa ccagccttcc aaaaacccaa atttgacgag cgagtgatgg
      961 agcatggcag ttccatcagc aaaccggaga tgaaactttt gttgaaccag atgggtgatt
     1021 tgaaatccat ttgattcaag cagggcggtg actcgatcaa ttgacaggcg atggtgttcc
     1081 tgctcggcca gtttgggcag gtagtgtggt ttgttgattc gaaccagcgt ttcacgaaag
     1141 acctggtaga cttcggtcat gtggccaaac aaattggtgg tcaggacaat tcgggcccgg
     1201 tcagccgcaa cacgaaaaca ctccttcatc accgcgtctg gatcggcaaa attgttgatt
     1261 cccaaatttg agacaatcaa atcaaaactg gcctcgggaa aaggcatctg actggcgtcg
     1321 gcttcgatga gttcgacatt ggtcaaacca tagatgtcgc gtttgtggcg ggcacggtca
     1381 agggccgctt tccaaatatc aacccccacc agcgtgcagg tgggtccatg gatgtgggcc
     1441 agctcaaaca gtggaaaccc ggtggcacag ccgatatcca gtatccgctg atgttttcgg
     1501 atgaccagat tgtcaatcag taaagcgcca aaacgggatg accaaaaaga cagttcgtca
     1561 aatacggatg ccagttccgg tttggtaaag tcaaagggct ggctgagttc agttcgcggt
     1621 ttgtcagtca tacagttcac accagaaaga aaaattggca actctgggta cgctccattg
     1681 caaaattgaa gcgattttgc ctgatttgaa gtttttttgc agacagtact tgaaagttcc
     1741 aggtg
//
LOCUS       DAOQDO010001563         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72758_length_1745_cov_2.040828, whole genome shotgun sequence.
ACCESSION   DAOQDO010001563 DAOQDO010000000
VERSION     DAOQDO010001563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72758_length_1745_cov_2.040828"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(638..979)
                     /locus_tag="PKZ53_20750"
     CDS             complement(638..979)
                     /locus_tag="PKZ53_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42925.1"
                     /translation="MASFNWIRVILSGLAAGFIMNCGEFVLNAVIFAKDIEESNRKLI
                     LPPPSGDFIAKAVILVFLVGIVAVLLYAAIRPRFGAGPKTAILAGFIIWFLVFVYQNQ
                     SLSVYKIVLNS"
     gene            complement(1142..>1745)
                     /locus_tag="PKZ53_20755"
     CDS             complement(1142..>1745)
                     /locus_tag="PKZ53_20755"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ42926.1"
                     /translation="HRIPVWAVAFSPDGQMLAVGGGGNVGIVYDVDTGQRLFELKGHT
                     NDINSIAFSPDGKRLATGSDDQTIRLWDMLRGSELALLDGHTGPVVSVAFSRDNQLLV
                     SGSRDRTVRVWDLATRRETLTLRGHRTNISSILFSPDGNRIVTGSQDGTTKLWDLQTG
                     QQLATLKGHSSWVNAVVFSPDGKILATGSQDSTVKLWQTE"
BASE COUNT          456 a          461 c          400 g          428 t
ORIGIN      
        1 ttcaaggata agtatttagg tttgctctaa aggatcaagg ttgggaagct ttgaaagtat
       61 tgtttcctga aaaaaacagt atctcaccac aaaggacaca gagaaagacc agcacactgt
      121 tgtatttcct ctgtgctcct ctgtgtcctc tgtggtgaaa atcttattca tctgcgtcaa
      181 acttcctgtt cccgatagac ccaggcgccg acgagcgtgg cgagtggggc ttcaaccagg
      241 gcccaggcca gtccgatgag ggtcaggttc aacgggaaaa ttcccatcgc attgtttaag
      301 acgctgccat agttccgaat tttattctaa tttataaaat cggtttcacc tggcggagtg
      361 gtctgtcacg attgataaac gacttctcgc cagccattcc actacgggga acggtgggtt
      421 atgggttata gaagaagttg atggtgctcc agaaaacgct ccaccgacac tccccccgtg
      481 tgtgggacag caaagccgtt gagttggtga tccccaaaac gtttggcgat cacgtcgctc
      541 acattgcaat ccgctcactt ctgaaatgat tataaattag taatatcaat cttgcaacct
      601 gaaacaattc gatttactct ccaaatcctt ataaatttta tgagttgagg acaattttat
      661 atacactcaa tgattgattt tgatacacaa acaccagaaa ccagattatg aacccggcga
      721 gaattgctgt tttcggcccg gctccaaagc gtggacgaat cgctgcatag agcagcaccg
      781 ccacaatgcc gaccagaaat accaggatga cggctttggc gataaagtct ccgctgggtg
      841 gcggaaggat gagtttgcgg ttcgattctt caatgtcttt ggcaaagatc accgcattca
      901 ggacaaattc accacagttc attataaatc cagcagccag tccgctaagg atcactcgaa
      961 tccagttaaa tgaagccatg ttcgttttcc tccgatgaaa tgattgatca tttcattgac
     1021 gaaccggctt tctgaaaatc gaccgcaccg aaaatttttg tcgaaaattt ttcaccacca
     1081 aaccgggcgc ggtacgccag gccgtaaacc gggcacagta cgcaaagccg tgcagtgccc
     1141 actactccgt ctgccacaac ttcaccgtgc tgtcctgact gccagtcgcc aaaatttttc
     1201 catctgggga aaacacaacc gcgttgaccc agcttgaatg ccctttcaaa gttgccagtt
     1261 gttgcccggt ttgaagatcc cacaatttcg tggttccatc ctggcttcca gtcacaattc
     1321 ggtttccatc gggcgaaaaa agaatggagc tgatgttggt tcgatgaccg cgtaacgtca
     1381 gtgtttcccg acgggtagcc agatcccaga ctcgaacggt tcgatcccgg ctgcctgaaa
     1441 ccagcaactg gttatcacgt gaaaaggcaa ctgacacgac cggaccggtg tgtccgtcaa
     1501 gcaaagcaag ttcactcccc cggagcatat cccagagtcg aatcgtctgg tcatcgctcc
     1561 cagtcgccaa ccgtttccca tctggtgaaa aggcaatgga gttgatgtcg ttggtgtggc
     1621 ctttgagttc aaagagtcgc tgaccagtat ccacgtcata cacaatcccc acatttcccc
     1681 ccccgccaac cgccagcatc tgcccatctg gggagaaagc caccgcccag actggaatcc
     1741 gatgg
//
LOCUS       DAOQDO010001564         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72786_length_1744_cov_3.779159, whole genome shotgun sequence.
ACCESSION   DAOQDO010001564 DAOQDO010000000
VERSION     DAOQDO010001564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72786_length_1744_cov_3.779159"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1744
                     /locus_tag="PKZ53_20760"
     CDS             <1..>1744
                     /locus_tag="PKZ53_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ42927.1"
                     /translation="ISGISLARGYLNQPELTAERFKTGFRVPGSGFRVSEPELTSQAP
                     DHEGEFASQRIEENSRLHNVEPGTRNPEPGTRTLYRTGDLARYRADGQIEFLGRIDDQ
                     VKLRGHRIELGEIEAVLNHHPLVRESVVMARDDAQSGKQLVAYLVLNQPTRPATGPLS
                     QNEQPGLSAEHHQELTDFLRHRLPEFMVPSVFVALDALPLSPGGKVDRRALPAPERLL
                     RTQTLEGEPLLRTPVEEMVADLWSQVLHQPVVHRHSNFFALGGHSLLATQLASRLRET
                     FQLDLPLSLIFESPTVVQQAAWITRQQMQGLPAESAIPPLVSVSREHPLPLSFSQLRT
                     WFMEQLEPSEPLTGGVRLQGKLDLMVLERCFVEIIERHEVLRTSFETVNGQPIQVIHR
                     TLRFQIEVINVQDAASPEERRARIEEFLAREGRKPFQLTTGPLLRVALIQLAPESSIL
                     FFAVHHIISDGWSLEIFGRELALLYRAFSKGEPSPLRPLPIQYADFAVWQRSWLQGDV
                     LKAHLNYWREQLTGAPAVLELPTDAPRPEVWAYRGMRETHTFSKTLTEKKKTLSQATG
                     VTMFMTLFAGYTVLL"
BASE COUNT          397 a          479 c          450 g          418 t
ORIGIN      
        1 catcagcggt atcagcctgg cccgtgggta tttgaatcaa ccggaattaa cggcggagcg
       61 atttaaaaca gggttccggg ttccgggttc cgggttccgg gtttcagaac cagagttgac
      121 ttcacaagct ccagatcacg agggtgaatt tgcatcccag agaattgagg aaaactcaag
      181 acttcacaac gtggaacccg gaacccggaa cccggaaccc ggaacccgta ctttgtatcg
      241 aaccggtgat ctcgctcgct atcgtgccga tggacagatt gaatttttgg gacgcattga
      301 tgatcaggta aagctccggg gacaccggat cgagcttggg gaaatcgaag ccgtgctcaa
      361 ccatcaccca ctggtcaggg aatcagtggt tatggctcgt gatgatgctc agagtggaaa
      421 gcaactggtg gcttacctcg tactcaacca gccgacacga ccggccaccg ggccactttc
      481 ccaaaacgag caacctggac tttcagccga acaccatcag gaactgaccg attttttgag
      541 gcaccggctg cctgaattta tggtgccgtc ggtctttgtt gcccttgatg ccctgccact
      601 ctcaccaggc ggcaaagttg atcgtcgagc cttaccagca cctgagcggc tgcttcgaac
      661 tcaaactctg gaaggtgaac ctcttctgcg aacaccagtt gaagaaatgg tggctgatct
      721 ctggtcccag gtcctgcatc agccggtcgt tcaccgccat tccaattttt ttgctctggg
      781 tggtcattcc ctgctggcaa cgcaactggc atcccggctt cgcgagacat ttcaacttga
      841 tctgccgttg agcctgattt ttgaatctcc aaccgtagtt caacaggcag cctggattac
      901 ccgtcagcaa atgcagggct tgccggctga atctgcgatt ccaccactgg tatctgtttc
      961 gagagaacat ccgcttcccc tttctttttc acagcttcgc acctggttta tggagcagct
     1021 tgagccgagc gaaccgttga ctggtggggt tcgattacag ggcaaactgg atctgatggt
     1081 tttggaacgc tgctttgtgg aaatcattga gcgtcacgaa gtgctgcgca catcatttga
     1141 aaccgtcaat ggccagccaa tccaggtcat tcaccgaacg cttcggtttc agattgaagt
     1201 catcaatgtt caagacgccg cctcaccaga agaacgccgg gcacggattg aagaatttct
     1261 tgcccgagaa ggccgtaaac catttcagtt gaccaccggg ccgcttctgc gagtggcatt
     1321 gatccagctt gcgccagaat cttcaattct cttttttgca gttcatcaca ttatttccga
     1381 tggctggtca cttgagattt ttgggcgtga actggccctg ctctatcggg ctttttccaa
     1441 aggtgagcca tcgccgctcc gaccacttcc catccagtac gctgattttg ccgtctggca
     1501 acggagctgg ctgcaaggcg atgtcctgaa agcacatttg aattactgga gagaacaact
     1561 taccggggcg ccagccgtgc tggaattacc aacagatgca ccacgccctg aggtctgggc
     1621 ctatcgcggc atgcgcgaaa cccacacgtt ttccaaaacg ttgaccgaaa aaaaaaaaac
     1681 gctcagtcag gcaaccggcg tgacgatgtt tatgacgctg tttgcgggct acacggtgct
     1741 cctg
//
LOCUS       DAOQDO010001565         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72794_length_1744_cov_3.246892, whole genome shotgun sequence.
ACCESSION   DAOQDO010001565 DAOQDO010000000
VERSION     DAOQDO010001565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72794_length_1744_cov_3.246892"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1744
                     /locus_tag="PKZ53_20765"
     CDS             <1..>1744
                     /locus_tag="PKZ53_20765"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNJ42928.1"
                     /translation="LIGLRVKPESLDRAEALTFEIIERLRRERISDADLQRAKSLLEK
                     DFLTRTESTQNQARTLAQVEATAGFKAWTTSLSRINQVTAEQIRAIAAKYLMLPKATV
                     HEFQSPTAPLRTFTPEKYAETVNILVPASTQTEIPSEAITDAKSVPMIRQGTTRQKQQ
                     DVGKFIVMAQAEPVKDFSTLRGPRAFVRVDPNRPLLVMGLFFQGGRTLEDETNSGVTE
                     LMLRSMLQGTAKQPGDTLASELEQLGGEIRIVNDPDYFGLELEILSRNGEEGLRRLVD
                     VIENPTFDKEAVKRERERLLADQQALTNAGSETALDLARSSLFGQHTYGFPRLGTAAV
                     AKMTEVDVQRWLERTVKRQYPLAVIVGDTEGSVLVSRVLVDGFNRDETDRSIKVRVIE
                     PNAKPAEQSGTSTRPVSYLNLVWLAPEGQKSVAAEWPVVAEMIASQLARQAEEAKLGG
                     AIFDSRYVPHLQYGEFILTAGTTVVEEPKLRSMVDTLIKGLATVEISESEWQGACRRA
                     ATREAQEIETFSGRARAYATAAFMVLPVAIVERNIENALRATSEDGKGVFSTLGQNQP
                     GRGVIRGTALPATPA"
BASE COUNT          432 a          428 c          504 g          380 t
ORIGIN      
        1 ttttgatcgg gttgcgagtc aaaccggaat cgcttgatcg ggccgaagcg ctgacgtttg
       61 aaatcattga gcggctccgg cgcgagcgga tttcagatgc tgatttgcag cgagcgaaat
      121 cattgttgga aaaggatttc ctgacccgaa ccgaatcaac tcagaatcag gcgcgaacac
      181 tggcccaggt tgaagccacg gcggggttta aagcctggac aacgtcattg tcacgcatca
      241 atcaggtaac agcggagcag attcgagcca tcgccgccaa atatttgatg ttaccgaaag
      301 cgactgtgca tgaatttcaa tcaccgaccg ctccgctgcg gacatttact ccggaaaaat
      361 atgccgagac cgtcaatatt ctggttccag cgtcaactca aactgaaatt ccatctgaag
      421 ccatcactga tgccaaatca gttcccatga tcaggcaggg aacaacccgt cagaaacagc
      481 aagacgttgg gaagttcatt gtgatggccc aggcagagcc agtgaaggat ttctcaacgc
      541 tgcgtggtcc acgggctttt gtgcgggttg accccaatcg gccattgctg gtgatggggc
      601 tgttttttca aggcgggcgc accctggaag atgaaaccaa tagtggtgtg acggaactga
      661 tgttgcgttc gatgttacag gggacggcca aacaaccggg cgatacactg gcgtcagaac
      721 tggagcaact gggtggggaa atccggattg tcaatgatcc agattatttc ggactggaac
      781 tggaaatcct ctcgcgaaat ggtgaagaag ggctgcgacg actggtggat gtgattgaaa
      841 atccaacctt tgataaagaa gccgtcaaac gcgaacgcga gcgtctgctg gctgatcaac
      901 aggcgctgac caatgcagga agtgaaaccg ccctggatct ggcccgatca agtctgtttg
      961 gccagcatac ctatggattt cctcgactgg gcaccgccgc tgtcgcgaaa atgaccgaag
     1021 ttgatgttca gcgatggctg gaacgtactg tcaaacgcca gtacccgctg gcggtcatcg
     1081 ttggcgatac cgaaggttcg gttctggtat cacgagttct ggtggatggg tttaatcggg
     1141 atgaaactga ccgttcgatc aaagttcgag tgatcgaacc caatgcaaaa ccagccgagc
     1201 aaagcgggac cagcacgcgt ccggtcagtt atctgaatct ggtctggttg gcgcccgagg
     1261 gtcaaaagtc agtcgctgcc gagtggccag tggtggctga aatgattgct tcacaactgg
     1321 ctcgtcaggc tgaagaagcc aaactcggcg gggctatttt tgacagccgc tatgtccccc
     1381 atttgcaata tggcgaattc attctcacgg ctggaaccac agtggttgaa gaaccaaagc
     1441 tcaggtcaat ggttgatact ttgatcaagg gattggccac agttgaaatt tccgaatctg
     1501 agtggcaagg tgcctgtcgc cgggcggcca cccgtgaagc tcaagagatt gaaacctttt
     1561 caggtcgggc gcgagcatat gccacggctg cctttatggt attgccggtt gcgattgtcg
     1621 aacgcaatat tgagaatgcg ctccgggcga ccagtgaaga tggcaaaggc gtcttttcaa
     1681 cgttggggca aaaccaacct ggacgcgggg tcatccgggg cacggctctg cccgcaacac
     1741 cagc
//
LOCUS       DAOQDO010001566         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72799_length_1744_cov_3.034932, whole genome shotgun sequence.
ACCESSION   DAOQDO010001566 DAOQDO010000000
VERSION     DAOQDO010001566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72799_length_1744_cov_3.034932"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PKZ53_20770"
     CDS             complement(<1..1537)
                     /locus_tag="PKZ53_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ42929.1"
                     /translation="MNVNLKSLIGKLNPTCRAVIEASAGLCLQRTHYDVEIEHLLIKL
                     LDVPNTDCIAIFRAFEVNQAQLGRDILRTLDRFKTGNGRTPALSPRIPQLIQEAWLVA
                     SVEFGVTSIRTGHLLLALLRNEQLARIGRETSRELLQISVEPLQRKFTEVTAGSLEAQ
                     AATTELNSPADQTQFNSQNSNSIKSAALDQFTIDLTARARKGEIDPVLGRDFEIRQVI
                     DILTRRRQNNPILTGEAGVGKTAVVEGLALRVAKGDVPEVLKKVAIRTLDLGLLQAGA
                     GIKGEFESRLKSVIDEVKASPQPIILFIDEAHTMIGAGGQAGQNDAANLLKPALARGE
                     LRTIAATTWAEYKKYFEKDAALARRFQVVKVEEPGEAKAMVMMRGIVESLETHHNVRI
                     LDEAIEAAVKLSHRYITGRQLPDKSVSVLDTACARVAIGQVATPPAIEDAERQIEQLT
                     TEIRVLERETVTGAAHDQRIRDLTTQRIEVEETRSRLKDQLDKESALINRIRDIRAHL
                     EALH"
BASE COUNT          385 a          461 c          451 g          447 t
ORIGIN      
        1 gatggagtgc ttccaggtgg gctcgaatgt cgcgaattcg attgatgagg gctgattcct
       61 tgtccaattg atctttcaaa cgtgaacgtg tttcttcgac ttcgatgcgt tgcgtggtca
      121 agtcacgaat gcgttgatca tgcgcagcac ccgtcacagt ttcgcgttcc aaaacgcgga
      181 tttccgtggt caactgctca atctggcgtt cggcatcttc aatggctggc ggcgtcgcca
      241 cctgcccgat ggcaacgcgg gcacaggcgg tatccaacac gctcaccgat ttatccggga
      301 gttgccgccc ggtaatgtaa cgatgcgaaa gttttaccgc ggcttcaatc gcttcgtcca
      361 gaatgcgaac gttgtggtgg gtttcgagtg attcaacgat gcctcgcatc atcaccatgg
      421 ctttggcttc gcccggttct tcgactttga ccacctggaa gcgtcgcgcc agcgccgcat
      481 ctttttcgaa atattttttg tactccgccc aggtcgtggc ggcaatggtt cgcagttccc
      541 cacgagccag cgcgggttta agcaggttgg cggcatcatt ttgacctgcc tgaccgccag
      601 caccgatcat ggtgtgcgct tcgtcaatga acagaatgat tggctgcggc gacgctttca
      661 cttcatcaat caccgacttg agccggcttt cgaattcgcc tttaattcca gccccggctt
      721 gcaagagccc caggtcgagc gtccgaattg ccactttttt gagcacttcc ggtacatcgc
      781 ctttggccac ccggagcgcc agcccttcga caaccgcagt ttttccaacc ccggcttcac
      841 cagtcagaat cgggttgttt tggcgccgac gggtcaaaat atcaatcacc tgccggattt
      901 cgaaatcacg gcccaacacc gggtcaattt cacctttgcg cgccctggca gtcaaatcaa
      961 tcgtgaattg atccaatgcc gccgatttaa ttgaatttga gttttgcgaa ttgaattgag
     1021 tttgatctgc cggtgaattc aattcagtgg tggcagcctg ggcttcaagg gagccagccg
     1081 taacttcagt gaacttgcgt tgtagcggtt caactgaaat ttggagcaat tcgcgcgagg
     1141 tttcgcggcc aatgcgggcc agttgttcat ttcggagaag tgccaggagc aggtgacccg
     1201 tgcgaataga cgtcacacca aactcgaccg aggccaccag ccaggcttcc tgaatcagtt
     1261 gtggaatgcg cggggagagc gccggagtcc gtccattgcc ggtcttgaac cggtccaacg
     1321 ttcgcaaaat atcacgtcca agctgggcct gattgacttc aaacgcccga aaaatcgcga
     1381 tgcaatcagt gttgggcaca tccagaagct ttatcagcaa gtgctcgatt tccacgtcat
     1441 agtgggttcg ctgcagacac aaaccggctg atgcttcgat caccgcccgg cacgttggat
     1501 ttaatttacc aatgagtgat ttaagattga cattcatcgc cttcgttcct accagagagt
     1561 tataccattt tgggatcagg ggctctggat cagggaaata caattttttc aataatttag
     1621 cctttccata atgcgagaac ttcagtccaa aatggtatta aattaaattt ctttgattct
     1681 gagtaagtgc aaatcgaagg ccagagtttg gggctgaaga ctcgcaagct cagggctgaa
     1741 gact
//
LOCUS       DAOQDO010001567         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72801_length_1744_cov_2.969805, whole genome shotgun sequence.
ACCESSION   DAOQDO010001567 DAOQDO010000000
VERSION     DAOQDO010001567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72801_length_1744_cov_2.969805"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKZ53_20775"
     CDS             complement(<1..300)
                     /locus_tag="PKZ53_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42930.1"
                     /translation="MPRFSFLLSAALILCLTGGGTPLTTTFGQSGRVHRQESADTSST
                     PMRSGSVVIPSGTILRLSLQTPINTKMNEVNDPVSGTLEDSFFLNGKLLFGKGTEF"
     gene            complement(496..843)
                     /locus_tag="PKZ53_20780"
     CDS             complement(496..843)
                     /locus_tag="PKZ53_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42931.1"
                     /translation="MPFSYFECPHCRQAGVHWFQKYRSADYGNELGLATCRRCQGKSF
                     TPGRNIPLSFGYLGTFLGLGTYLVLPGAQSVRATIAVSLMVGSVVVCAYLQLKKPLLK
                     KEKPSRKKEPKPR"
     gene            complement(950..>1744)
                     /locus_tag="PKZ53_20785"
     CDS             complement(950..>1744)
                     /locus_tag="PKZ53_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42932.1"
                     /translation="AMRPPMTSPFYGTAPLAPPQNYGTQPLAPPNPYPQATQPLSGYG
                     TSPLAAPPMSGPTSSSTGSYPAFGSTMPLQQPTGTLSAPPKPRMPRPVAYGTPGPTTP
                     PPVEDPADPMSYANREGDKWPEATMTRRPQWTDPSKSTVNLDAAIADLLAYLDDQKAP
                     LFVADSLSILFRTAAPFYMKRERVAEIVAGWALRRASLSGKPIHELMLAAVNNIKHAE
                     QARVLTQFEGPKFYEDFIRELLTYCPPEEHEIFYIKLGGIKELLRK"
BASE COUNT          420 a          396 c          494 g          434 t
ORIGIN      
        1 aaattcagtt ccttttccaa acaacagctt gccgttcaag aagaacgagt cttcaagtgt
       61 tcccgaaacc gggtcgttga cttcgttcat tttcgtgttg attggtgttt ggagtgacaa
      121 ccgaagaatc gttccagagg gaatgacgac ggatccgctt cgcatcggag tagacgatgt
      181 gtctgccgat tcctgacggt gaacgcggcc actttgaccg aatgttgtgg taagtggagt
      241 ccccccaccg gtgagacaga gtataagcgc ggctgataat aaaaaagaaa atcgtggcat
      301 aatggatctt caactttctc aaagcaatag agtgctcaga acttccgcct ttttcaaggc
      361 aacctcaagt gatttgggct gatgtgtgga atcgtgttag ccgaccaccc atactacctt
      421 acctgttggc gacttgtcta aatgcttctg aaaaaagatg gggaagttgg ggtggtgatg
      481 actggttaca cttcatcagc gtggcttcgg ttcctttttt cgagatggtt tctctttttt
      541 gagaagcggt ttcttcaatt ggagataggc acacaccacc acgctcccaa ccattagtga
      601 aacggcaatc gtggcccgaa ctgattgcgc tccgggaagc accagatagg tcccaagtcc
      661 taaaaaggtc ccgagatatc caaatgaaag cggaatattt cggcctgggg taaacgattt
      721 tccctgacag cggcgacagg ttgccaggcc aagttcattg ccataatccg ctgaacggta
      781 tttttgaaac cagtgcacgc cagcctgacg gcagtgagga cactcaaaat aagaaaaagg
      841 catagggagg ataaagagcg aagtagccag gtaacaagtg ttgattggca ggtttgtttt
      901 gttaatccaa aatttctatg taatttctat ttggctactt cacgaaaaat tatttgcgca
      961 acaattcttt gattccgccg agttttatgt aaaagatttc gtgctcttcc ggtgggcagt
     1021 aggtcaaaag ctctcgaata aagtcctcat agaatttggg gccttcaaac tgggttagaa
     1081 cccgggcctg ttcagcatgc ttgatattgt tgacggcggc cagcattaat tcatggatgg
     1141 gtttgccgga taaactggcc cgccgcaaag cccagccagc cacaatttcg gcgacccgtt
     1201 cacgtttcat ataaaatggg gcagccgtcc gaaacagaat tgataacgaa tcggccacaa
     1261 acaacggagc tttttggtcg tcgaggtacg ccagcagatc ggcaatggcg gcatccaggt
     1321 tgacagttga tttacttgga tcggtccatt gtgggcggcg ggtcatggtt gcttcaggcc
     1381 atttgtcacc ctcgcggtta gcatatgaca tcgggtctgc cgggtcttca actggaggtg
     1441 gtgtggttgg ccctggggtt ccataggcga ccgggcgagg catgcggggc ttcggcggtg
     1501 ccgacagtgt gccagttggt tgctggagcg gcatggttga gccaaaggcc ggatagctcc
     1561 cagttgaact cgatgtcggt ccagacatgg gcggtgctgc cagtggggag gtcccatagc
     1621 cactcaaggg ttgtgttgcc tgaggataag gattgggcgg cgccagcggt tgggttccat
     1681 aattctgagg cggggccaac ggcgcggtgc cataaaacgg agacgtcatc ggaggacgca
     1741 tggc
//
LOCUS       DAOQDO010001568         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72817_length_1744_cov_2.486679, whole genome shotgun sequence.
ACCESSION   DAOQDO010001568 DAOQDO010000000
VERSION     DAOQDO010001568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72817_length_1744_cov_2.486679"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..>1744
                     /locus_tag="PKZ53_20790"
     CDS             275..>1744
                     /locus_tag="PKZ53_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNJ42933.1"
                     /translation="MLYRLLRSALLCLAGGLAFFIFLSGSSSIPVVQGQVFRGGSYQI
                     EQRITLDPLSAQSGGPKDLAIDETTNRVYIIYQDSSLIAVLDGATDTITAYIDAGRPV
                     GKMVLHPERQQLYVTHFAENAVSVIDLTQQKVIQVIDNLQSPEGIAVNTQTQELFVQS
                     QFQIQVIDGLTWKMTRSFDLPAKERSIFAINSKNNHLYWILPGGEVDTDQFEPAVLYV
                     IDAATGSTLLTHRLGTGNYWRKIEADSLVLDPTNDLLFIQNAHLGYLFWSAVITVVKT
                     TDYSSWKYLGGEGALCFGYNPNTGHLFANNWLVDDSCQGANVMVNSLDTGENVASIPT
                     GLGFPYGVGVNTRTNRVYVITGYWRLLIIDDITLTAVKSLPLKLFVSDVAINPAMNQV
                     YVSIFANKTIVILDGTSQKQIGEIQLDRHPEEIRFSAEKNRLYVETFQGSSCNWYPWL
                     TVIDVPTRRVLSSYPVQTYLTNLRFHPTRPWIYQPTESGL"
BASE COUNT          451 a          425 c          392 g          476 t
ORIGIN      
        1 gtgatcaccc cgctgtcatc gccacgtgtc actacgggcc tggccgggtt gtcaattgtt
       61 ttttgcctca cgctggatag atgaaccatc cgcaacctgc gctgaagctg acttcaaacc
      121 ggtcgtcagg ccagccaccc aaaataagaa cctgggttgc gcaagattta acattgttaa
      181 caatcatttt gcatcggctt aacctttctc gaaagaaacg gtttatgatg gcggccacgg
      241 tgtcacatct ttccttcggt ttaaggaaca aactatgttg taccggctgt tacgcagcgc
      301 gctcctgtgt ttggcaggtg ggcttgcttt tttcatcttt ctttcagggt cgagttcaat
      361 tcctgttgtt caaggacagg tcttccgagg tggctcttac caaatcgaac aaagaattac
      421 tttagatcca ctcagcgctc aatcaggagg tcccaaagat ttagccattg atgaaaccac
      481 caatcgggtg tacatcatat atcaggactc atcgctgata gcagttctgg acggggcaac
      541 cgataccatc acggcatata tagacgcggg cagaccggtt ggaaaaatgg tcctccaccc
      601 agaacgccaa cagctctatg tcacacactt cgcagaaaat gccgtttcag tcattgacct
      661 cactcagcaa aaagtcattc aggtcattga caatcttcag tcacctgaag gaattgcggt
      721 taatacccaa actcaagaac tctttgttca gagccagttt cagatccagg tcattgatgg
      781 ccttacctgg aaaatgaccc gctcatttga ccttcccgca aaagagagaa gcatctttgc
      841 gatcaactcg aaaaacaacc atttgtactg gatcctccct ggtggcgaag ttgataccga
      901 ccagtttgaa ccagcagttt tgtatgtcat tgatgcagca actggctcaa ccctgctgac
      961 acaccggctt ggtactggca attactggcg aaaaatagag gcagacagcc tggtgcttga
     1021 tccaacaaac gatttattat ttattcaaaa tgcccatctg ggctatcttt tctggtctgc
     1081 ggttattaca gtggtgaaga caactgatta ttcgagttgg aagtacctcg gcggtgaagg
     1141 cgcactttgt tttggttata acccaaatac agggcatctg tttgccaata actggctcgt
     1201 tgatgatagt tgccagggcg cgaatgtcat ggtcaatagt cttgacactg gagaaaatgt
     1261 ggcctccatc ccgaccgggt tggggttccc atatggagtt ggcgtcaaca cgcggaccaa
     1321 tcgggtgtat gtcattactg gatattggcg gctcctgatc attgacgata taaccttaac
     1381 cgctgtcaaa tcattaccat taaagctatt tgtgtctgac gtggcgatca atcctgcaat
     1441 gaaccaggtg tatgtttcaa tttttgcgaa taaaaccatt gtcatccttg atggtacttc
     1501 ccaaaaacag attggtgaaa tacaactgga tcgtcatccg gaagaaatac ggttttcggc
     1561 tgaaaagaat cgtttgtatg ttgaaacatt tcagggaagt tcgtgtaatt ggtatccctg
     1621 gctgactgtc attgatgtac caacccgccg tgttttatcc agttatccgg tacaaacata
     1681 ccttaccaac ctgagatttc atcccactcg tccctggatc tatcaaccca ccgagtcggg
     1741 cctc
//
LOCUS       DAOQDO010001569         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72866_length_1743_cov_3.197275, whole genome shotgun sequence.
ACCESSION   DAOQDO010001569 DAOQDO010000000
VERSION     DAOQDO010001569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72866_length_1743_cov_3.197275"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKZ53_20795"
     CDS             <1..295
                     /locus_tag="PKZ53_20795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ42934.1"
                     /translation="VPVGLILNELLTNILKYAFPDNRQGKIQISLEPDAEKRLILTVS
                     DDGVGMPDTVKVQSPTTLGLRLVKILAKQLAAEVTFHTSTAGTSFQFLFPDLN"
     gene            345..947
                     /locus_tag="PKZ53_20800"
     CDS             345..947
                     /locus_tag="PKZ53_20800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42935.1"
                     /translation="MPQARILIVEDEGLIAADIEDRLTRLGYQVDGWASTGEEAIKNI
                     DACLPDLILMDIMLRGNLDGIAVAEHVHHHYQIPVIFLTAYSDETMIERAKRAEPFAF
                     LLKPFNERELRSNIEMTLYKHRMEQERNQLQAKLAEAQQQLKVLSGLLPICSFCKKIR
                     DDQGYWSQLEEYITDHSEAQFTHSLCPGCVRIHYPQYSDS"
     gene            1334..>1743
                     /locus_tag="PKZ53_20805"
     CDS             1334..>1743
                     /locus_tag="PKZ53_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNJ42936.1"
                     /translation="MTDTQTKKSSQAVNIPPATGKLGVLLVGLGAVSTTFIAGVEAIR
                     RGTAQPIGSLTQMASIRLGKRTEGRNPRIKDFVPLAELDDLVFGAWDLFEDSAYEAAV
                     HAGVLERTQLDQLKPALEAIKPMPAVFDQHYVKRL"
BASE COUNT          461 a          436 c          412 g          434 t
ORIGIN      
        1 ggttccagtg ggactgattt tgaacgaact cctgaccaat attctcaaat atgccttccc
       61 tgataatcgc cagggcaaga ttcaaattag tctcgaacct gatgcggaaa aacgtctgat
      121 actgactgtt tcagatgatg gcgtcggaat gcctgacacc gtgaaagttc aatcaccaac
      181 gacattaggg ctccgattgg ttaagatttt ggccaaacaa ctggcagcag aagtaacctt
      241 tcacaccagt actgctggca cttcatttca gtttctcttt cctgatctca attagaccgg
      301 gagaccattt tcattcaatt tattcagctt ttagggggtt ggttatgcct caagcacgca
      361 tcttgattgt ggaagacgaa ggcctgatcg ctgctgacat tgaagaccga ctgacccggt
      421 taggttatca ggttgacggc tgggccagca ctggcgagga agccatcaaa aacattgatg
      481 cctgtctacc ggatctcatc ctgatggata tcatgctgcg aggaaatctt gatggcattg
      541 ccgtggctga acatgtccac catcactatc aaatcccggt gatctttctg actgcctatt
      601 ccgatgaaac catgattgaa cgggctaaac gggctgagcc atttgctttc ttgctgaaac
      661 cttttaatga gcgggagttg cggagcaaca ttgaaatgac gctctataag caccggatgg
      721 agcaagagcg caaccagtta caagccaaac ttgccgaagc tcagcaacaa ctcaaagtgc
      781 tctctggctt attgccaatt tgctcgttct gcaaaaagat tcgcgacgat cagggatact
      841 ggagccaact cgaagaatat atcaccgatc attccgaagc ccagtttacc catagcctgt
      901 gcccagggtg cgttcggatt cactacccac aatatagtga ctcgtgacgg aatgagcgag
      961 tgagtagcaa gttttcactc cgtaagtgcc aaaccgtgga aggatcaatt ccattgaaaa
     1021 ccaaacggca aagtcaccct gccattttgc agtaactccc tgataaatca atattttctg
     1081 tgttgttctt gatcatcagg agtcaaggta ggacagagat gcctgtcaac acaggcttca
     1141 gctttcgcct tgcactcaca ggtccagtgc ttatatcttt gcacccccaa aaattcgaga
     1201 cgagacgaga ttcagactga ttccctgcaa ccaaacgctg aaaaacctgc cacgatatga
     1261 taaacaccgc aaacaatcat tcctcttgtc atttttcttt ctggaatcgc tgaatttgct
     1321 tagaaggagc agtatgaccg atacacaaac caaaaaaagt agccaggctg tgaatatccc
     1381 ccccgcaacg gggaagcttg gcgtgttgct ggttggattg ggcgccgtga gtacgacgtt
     1441 catcgccggc gttgaagcca tccgacgtgg gacggcccag ccaattggat cactgaccca
     1501 aatggcatct atccgccttg gaaaacgcac cgaagggcgc aaccctcgca ttaaagactt
     1561 tgttccgctg gccgaacttg atgacctggt gtttggtgcc tgggatttgt ttgaagactc
     1621 tgcgtatgaa gcggcggtcc atgccggcgt tttggaacgg acccagcttg atcaattgaa
     1681 accggcgctg gaagccatca aacctatgcc agccgtgttt gaccagcatt atgtgaaacg
     1741 gct
//
LOCUS       DAOQDO010001570         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72869_length_1743_cov_3.047393, whole genome shotgun sequence.
ACCESSION   DAOQDO010001570 DAOQDO010000000
VERSION     DAOQDO010001570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72869_length_1743_cov_3.047393"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /gene="cas3"
                     /locus_tag="PKZ53_20810"
     CDS             <1..1337
                     /gene="cas3"
                     /locus_tag="PKZ53_20810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNJ42937.1"
                     /translation="EIDRELLIEDVLHTNEDQHEPSGEKAESLDSRLASLEAWPNKLI
                     VCTVDTVLGLIQNNRRPLYSWPALCQSAFVFDEVHAYDNQLFGALLRFLETFRKTPIL
                     LMSASFSESQLHAIQALVEIKLGESLEIIDGPQNLEKIQRYQIQVLNTQDSAWDEIEQ
                     TLKEGSKVLWVTNIVSDTRNNKVGCIDIFREACQRLAPLGIRPLIYHSRFRYEDRVQK
                     HKDLVNAFAGEGPVFAVTTQVCELSLDLSATLLVTASAPAAALIQRFGRLNRKVEELP
                     NGQIRLRSGKVCPALVYPWKFEFPYEAEELATGTQLLDCLPSGPISQMDLAVVAAGLE
                     SQPVGPVTSSWLDKNWRSFPTSLREAGNTLTVLLEEDLPAIRQAVREKNVPLSVEAQR
                     WTVPITILKDSIHWQREGVYPIAPKGLIVYSKETGAEICKQNQKSNSTLVIL"
     gene            1337..>1743
                     /gene="cas8a1"
                     /locus_tag="PKZ53_20815"
     CDS             1337..>1743
                     /gene="cas8a1"
                     /locus_tag="PKZ53_20815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated Cas8a1/Cmx1"
                     /protein_id="HNJ42938.1"
                     /translation="MTLLHRAGLAGLWMTLREFETRQLQGPGNFRWELTNRSVSLDWD
                     CKDFEALDWLLKKAFSLDQGLITFTGLKGTDLQNRVLFQQGLRGTFLQHSQTFQKEGI
                     ATRTFHLDENGAEITFNYQFLSWFIVLNARRHQ"
BASE COUNT          471 a          408 c          429 g          435 t
ORIGIN      
        1 ctgagattga tcgagaatta ctgattgaag atgtcctgca caccaatgag gaccagcatg
       61 aaccatctgg tgaaaaagct gaaagcctgg attcacggct ggcgtcactg gaagcgtggc
      121 caaacaagct gattgtctgc accgtggata cggtgctggg tttaatccaa aacaaccgtc
      181 gtccgttgta ttcgtggccc gctctgtgtc aatcggcctt tgtttttgat gaagttcatg
      241 cctatgacaa tcaactcttc ggggcattac ttcggtttct tgaaactttt cgaaagaccc
      301 cgattttatt gatgagtgcg tcattttcgg aaagtcagct ccacgcgatt caagcattag
      361 ttgaaataaa gcttggtgaa tcacttgaaa tcattgatgg cccacaaaat cttgaaaaga
      421 ttcaacgcta tcaaattcag gtacttaaca ctcaagattc agcctgggat gaaatcgaac
      481 aaaccctcaa agaaggcagc aaagtgttgt gggtgaccaa tatcgtgtca gacacaagaa
      541 acaacaaagt tgggtgtatc gacattttcc gggaagcctg ccagcgactg gctcctttgg
      601 gaatcagacc gctgatctac cacagccggt ttcggtatga agaccgggta caaaaacata
      661 aagacctggt gaacgctttt gctggcgagg ggccagtatt tgccgttacc acccaggttt
      721 gtgagctgag cctggatctg agcgcgacat tgcttgtcac ggcgtctgcc ccagcggcgg
      781 cgttgattca acgatttggg cgactgaatc gaaaagttga agaattacct aacggacaaa
      841 tcagacttcg atcaggaaaa gtgtgtccgg ccctggttta tccctggaag tttgaatttc
      901 cttacgaagc cgaagaactg gcaactggaa cccaacttct tgactgtctt ccatctgggc
      961 cgatttccca aatggatctg gctgtggtgg cggcaggtct cgaaagtcaa cctgttgggc
     1021 cagtcacctc aagctggctg gataaaaact ggcgatcatt cccaacttcg ctcagagaag
     1081 ctggaaacac gcttacggtt ttgttggaag aagacctccc ggcaatccgg caggcagttc
     1141 gtgaaaagaa cgttccgctc tcagtcgaag ctcaacgctg gaccgttcca atcacaattc
     1201 tgaaagattc tatccactgg caacgagagg gcgtgtatcc aattgcaccc aaaggactta
     1261 ttgtgtatag caaagaaacg ggggctgaaa tatgcaaaca gaaccaaaaa tcaaactcca
     1321 ccttggtgat cctttaatga cacttttgca ccgggctggg ctggcagggt tatggatgac
     1381 cttgcgtgaa tttgaaaccc gccagcttca aggcccaggg aatttccgct gggaactcac
     1441 caatcgaagt gtctccctgg attgggattg caaagatttt gaggccctgg actggctttt
     1501 gaaaaaagct ttttcgcttg accagggctt aattaccttc actggcttga aaggaactga
     1561 cctgcaaaat cgggttttat tccagcaagg attgcggggg acttttttgc aacattcgca
     1621 aacctttcaa aaagaaggga ttgccactcg cacgttccat ctcgacgaaa atggagcgga
     1681 aataacattt aactatcagt ttttatcctg gtttatcgtg ctcaacgccc gaaggcatca
     1741 gag
//
LOCUS       DAOQDO010001571         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_72989_length_1742_cov_2.011855, whole genome shotgun sequence.
ACCESSION   DAOQDO010001571 DAOQDO010000000
VERSION     DAOQDO010001571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72989_length_1742_cov_2.011855"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1742)
                     /locus_tag="PKZ53_20820"
     CDS             complement(<1..>1742)
                     /locus_tag="PKZ53_20820"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ42939.1"
                     /translation="LITVLSPLGTGTSSDNFTVQSNTPTITSFTPTTGPPGTQVTITG
                     TNLTGATEVSFNTTSVGTNFTVVSATQINTTVPVGATGGPIKVKTPSGEATSPQSFIV
                     TSGAPPTISSFSPAMGPVGTVVTIEGNNFVGTTSVRFNATESLEYSVVSFTRLTAKVP
                     AGATSGAITVRTPDGAVTSTASFQVDTSLRPLINSFSPGRGPVGTFVIIKGVNFVDIT
                     SVKFGDVPVNSFKVVSSTELTTGVPAGAKDAQIVIENRAGVASSSKQFKIRTPDPTRC
                     KVKVPEERWRGEYYPNRNLDGKALAVRDDTNEEGFIKLAWAGSPAGVCGIPEDGFSVR
                     WTRKVTFPERAVYRFRTKADDGVRLYIDSTRILNRWEDQGPTEFTVDWDLTAGEHTLT
                     MEHYDRAGGAVAEFSWTPAPPEISDFSPKSGQVGDEVKIQGKNFAGELDVRFNGKPTQ
                     ILRGSTTEIVVAVPIAATSGPITVINPGGSAVTSQEFTVSGCNSLESIQRGQTIAGSL
                     ETSDCRSFIRGRNYFADRYQFKGKTGDLIAIQVDTPSVNTYVYLLRVDGTIVTENDDA
                     GGTLGSRIPAGPGF"
BASE COUNT          415 a          465 c          449 g          413 t
ORIGIN      
        1 agaagcctgg tcctgccgga attcgagatc ccagggtgcc accggcatca tcgttttcag
       61 tcacgatagt gccatcaacg cgaagtaaat agacatacgt attgacactt ggggtgtcaa
      121 cctgaatggc aatcaggtcc ccggttttgc ctttaaactg gtaccggtca gcaaagtaat
      181 ttcttccacg aataaaggat ctgcaatcac tggtctcaag cgagccagca atggtctgcc
      241 cacgttgaat ggattcgaga gaattacacc cactcaccgt aaactcttgt gaggtaacgg
      301 cactcccgcc tggattgatg acagtaatcg ggcccgatgt cgccgcaatt ggaacggcca
      361 ccacaatttc ggtggttgaa ccacgcaaga tctgagttgg ctttccatta aatcgcacat
      421 ccaattcacc agcgaagttt ttgccctgaa ttttgacttc atcgccaact tgcccactct
      481 taggcgagaa atcagagatt tccggcgggg ccggagtcca ggaaaattca gccacggcac
      541 cgccagcccg gtcatagtgt tccatcgtca gggtatgctc acccgctgtc aggtcccaat
      601 caaccgtaaa ttccgttggg ccttgatcct cccatcggtt gaggatgcgg gttgagtcaa
      661 tatagagccg gaccccgtcg tcggctttgg ttcggaaccg gtacacggcc cgttcaggaa
      721 aggtcacttt gcgagtccag cgaactgaaa agccgtcttc aggtatgccg cacaccccgg
      781 ctgggctccc cgcccaggcc agtttgataa atccttcttc attggtatcg tcacgaaccg
      841 ccagtgcttt tccatcaagg ttgcgatttg gataatattc acccctccac cgctcttccg
      901 gtactttcac tttgcatcgg gttgggtcag gagttctgat tttgaattgt ttggaactgc
      961 tcgccacacc cgctcgattt tcaatcacaa tctgggcatc tttggcgccg gcgggaacgc
     1021 cggtggtaag ttcagttgag gacacaacct taaatgagtt tacaggcaca tctccgaatt
     1081 tcaccgacgt aatatcaaca aaattcacgc ctttaatgat cacaaaggtg ccaactgggc
     1141 cacgaccggg tgaaaaactg ttgatgagcg gtcgtaacga agtatcaacc tgaaatgaag
     1201 ccgtgctcgt cacggcccca tcaggcgttc ggacggtgat ggcgccactt gtagcaccag
     1261 ccggtacttt ggccgtgagt cgggtaaacg aaacaaccga gtattccagg gattcagtcg
     1321 cattaaatcg aactgaggta gtcccaacaa aattattacc ttcaatcgta accacagttc
     1381 cgaccgggcc catcgccggg gaaaaacttg aaatcgtcgg aggtgcccca gaggtaacaa
     1441 taaatgactg gggactggtt gcttcaccag atggcgtttt caccttgatc ggcccgccgg
     1501 ttgccccaac cggcaccgtt gtgttgattt gcgtcgccga aacaaccgta aagttggtcc
     1561 caacactggt cgtgttaaac gacacctcgg tggcgccagt caaattggtt ccggtaatgg
     1621 tgacctgtgt tcccggagga ccggtggtcg gggtaaatga ggtaatggtt ggggtatttg
     1681 attgaacggt gaaattgtct gaactggtcc cggtgccaag tggcgacagg accgtgatca
     1741 ac
//
LOCUS       DAOQDO010001572         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73100_length_1740_cov_2.947181, whole genome shotgun sequence.
ACCESSION   DAOQDO010001572 DAOQDO010000000
VERSION     DAOQDO010001572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73100_length_1740_cov_2.947181"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /gene="uvrC"
                     /locus_tag="PKZ53_20825"
     CDS             complement(<1..1585)
                     /gene="uvrC"
                     /locus_tag="PKZ53_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNJ42940.1"
                     /translation="MHLDEKVSSLPTSPGCYLHKDKSGRIIYVGKAKNLRNRVRSYFQ
                     ESRGYDAKTRELVSRIADFEFIVTDTEIEALILESNLIKEYKPRYNVMLKDDKQYPHL
                     KLTLNERFPRVIKTRRVLNDKALYHGPYLPASLAQQTLKLINQLFQLRTCEIEIDGKR
                     ERPCLEYHIKRCLGPCVKDLCSKTQYNEAVKDVKLFFEGKKQDLIDSLRERMEQASEN
                     LRFEQAARYRDQIKLMERLGEEQKMMLKTAADIDIFGYFREGPRAALQLFTMRDGKII
                     GRREFFWEDLPLEGFDPRDFIGDALKQYYSLGNYVPSEAHVPVDFEDRPLLEEFLTQR
                     RGTRVKILDPQRGPKRDLIDLVERNAKISFDQRFRVLKPDMQKVLEDFADTLELPCFP
                     GRIECFDISHIQGAETVASMVVCDNGVMNKAEYRKFMIKTVEGIDDFKSMKEVVGRRY
                     KRLLREEKPLPDVVLIDGGKGQLSAAAAAMRQLGLEAVPMVAIAKREELLFVKGREQD
                     PVRIDHHSPLIHLVQMVRDE"
BASE COUNT          368 a          450 c          422 g          500 t
ORIGIN      
        1 cttcgtcacg caccatctgc accagatgaa tcagcggcga atgatggtca atccgcactg
       61 gatcctgttc ccgcccttta acgaagagca attcctcgcg cttggcaatc gccaccattg
      121 gtacggcttc aagtccaagc tgacgcatcg cggcggcggc ggctgacaat tgtcccttcc
      181 ctccgtcaat cagcacgaca tccggaagcg gtttttcttc ccgcaagagc cgcttgtagc
      241 gacgtccgac gacttctttc attgatttga agtcatcaat cccttccact gtcttgatca
      301 taaacttgcg gtattcggcc ttgttcatca cgccgttgtc acacaccacc attgacgcca
      361 cggtttcagc cccctggatg tgcgagatat caaagcattc aatccggcct ggaaaacaag
      421 gaagctccag cgtgtcggca aagtcctcga gcactttctg catatcgggt ttgagcactc
      481 gaaaccgctg gtcaaacgaa attttggcgt tgcgttcgac cagatcaatc aaatcgcgct
      541 ttggaccgcg ttgcggatcg agaattttca ccctggtgcc gcgccgctgg gtgaggaatt
      601 cctcaagcaa tgggcggtct tcaaagtcaa ctggaacgtg ggcttcactt gggacatagt
      661 tgcccaggct gtaatactgt ttgagtgcat caccaataaa atcacgcggg tcaaaccctt
      721 caagcggcag atcctcccag aaaaattcac gccgcccgat gatttttccg tcacgcatgg
      781 taaacaactg gagcgcagcg cgcgggcctt cgcgaaaata gccaaaaata tcaatgtctg
      841 ccgccgtttt gagcatcatt ttttgctctt cgccaagacg ttccatcaat ttaatctgat
      901 cccggtaacg agcagcctgt tcaaaccgga gattttctga cgcctgttcc attcgctcgc
      961 ggaggctgtc aatcagatcc tgctttttgc cttcaaaaaa cagcttcacg tctttaactg
     1021 cttcattata ctgggttttc gaacaaagat ctttgacaca gggcccaagg cagcgtttga
     1081 tgtggtattc cagacagggc cgttcgcgct tgccatcaat ttcgatttca caggttcgga
     1141 gttgaaataa ttgattgatg agctttaagg tttgttgcgc cagtgacgcc ggcaaatagg
     1201 gcccgtggta aagcgccttg tcgttgagca cgcgacgggt tttaatcact cggggaaatc
     1261 gctcatttaa tgttagcttc aagtggggat actgtttatc gtccttgagc atcacattgt
     1321 agcgaggctt gtattcctta atcagattgc tttccagaat taaagcttcg atttccgtat
     1381 cagtgacgat gaattcaaag tcagcaatgc gtgacaccag ttcgcgggtt ttggcatcat
     1441 acccccgcga ttcctggaag tagctgcgaa cccgattgcg caggtttttc gcttttccaa
     1501 catagataat ccttccggat ttatccttgt gtagataaca tcccggactg gtcggtaaac
     1561 tggagacttt ttcgtcaagg tgcataggct cgggcctgac ttctggtttt tggttcctgg
     1621 ttcctggttt ttggttcctg gttcctggtg cttggttcct ggttcttggt tcctggttct
     1681 tggttcttgg ttcttggttc ctggttcttg gttcttggtt cttggttctt ggttcttggt
//
LOCUS       DAOQDO010001573         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73101_length_1740_cov_2.946588, whole genome shotgun sequence.
ACCESSION   DAOQDO010001573 DAOQDO010000000
VERSION     DAOQDO010001573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73101_length_1740_cov_2.946588"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PKZ53_20830"
     CDS             <1..1357
                     /locus_tag="PKZ53_20830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ42941.1"
                     /translation="GYDSVADFPNKPLYYFQIVGANGVSVEDATTFKARQKEFALFAQ
                     DSWRIRSNFTLNLGLRWEGTTNPQPTRPNPAFASTSSVPNDYNNFAPRIGLAWDPKGD
                     GKQVIRADFGLNYGRVPAIYLFQAFNTNGVTNGAFFFFGDVPVTFPQTLPTSIPDAPL
                     GSDVFAFDPNFELTRSGMVNVSYERDLGRNFSFLATFTGSRTRNVPFVQNLNLASGFI
                     DTTGRFVYDRSVTPFPDYGRVFNLTSLGYDEYKALTLGVQKRFSNRFQFQANYTLSRT
                     KDTVSEEQPLFDIAQSNQFDPGYEFTTSARDIRHRFIANSVFDLPYGFRLSGIFTALS
                     GRPIDEVTGEDLNNDGNSGNDRVLGPDGRIRERNSQRGPAFYNFDVRVSKTFNLKDTA
                     SIEVLAEVFNLFRADNLGSNGTTNDGFSVVRRDTPAGPNSVLNSAGSPQQMQLGVRFR
                     F"
     gene            1413..1619
                     /locus_tag="PKZ53_20835"
     CDS             1413..1619
                     /locus_tag="PKZ53_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42942.1"
                     /translation="MNNRKTPSGFRLSEKAKLLLLALSQKLGISQTAVIEIAVRQMAE
                     TEHIELEKVNVREGLDETIITSKS"
BASE COUNT          411 a          462 c          441 g          426 t
ORIGIN      
        1 tggctatgac agcgtggctg acttcccaaa caagccgctc tattacttcc aaatcgttgg
       61 tgccaatggt gtttcggtcg aagatgcaac gacgttcaag gcccgacaaa aagaatttgc
      121 cctctttgcc caggatagct ggcggattcg gtccaatttc acgttgaatt tggggttacg
      181 ctgggaaggc accaccaatc cacagccaac gcgccccaac ccggcctttg ccagcacgtc
      241 atcggtgccc aatgattaca acaactttgc cccacgcatc ggcctggcct gggatccaaa
      301 aggtgatggg aaacaggtca ttcgggctga ctttgggctg aattatggcc gggtgccggc
      361 gatttatctg ttccaggcat tcaacaccaa cggcgtcacc aacggggcgt ttttcttctt
      421 tggcgatgtc ccggtgacat tcccgcaaac cttgccaacc agtattcccg atgcaccact
      481 tggttcagat gtctttgcct ttgatccaaa ttttgaattg acccgttctg ggatggtcaa
      541 tgtcagctat gaacgtgatt tgggacgcaa cttcagcttc ctggcgacct ttaccggtag
      601 ccgaacgcgt aatgtgccgt ttgtccagaa cctcaatctg gcgagtgggt ttattgacac
      661 caccgggcgc tttgtctatg accggagcgt gacgccgttt ccagattatg gccgggtctt
      721 taacctgacc agtctgggct atgacgaata caaagccctg acgctcggcg tgcaaaagcg
      781 gttttcgaat cggtttcagt tccaggccaa ctacacgttg agccgcacca aagacacggt
      841 ttctgaagaa cagccactct ttgacattgc ccaaagcaat cagtttgatc cggggtatga
      901 atttacaact tcggcgcgag acatccgcca ccggtttatt gccaacagcg tcttcgacct
      961 gccctacgga ttccgacttt cggggatttt cacggcactg agcggtcgcc caattgacga
     1021 agtaaccggc gaagacctca acaacgacgg caacagcggc aatgaccggg tgctggggcc
     1081 ggatgggcgc attcgtgagc gaaatagcca gcgcgggccg gcgttttaca acttcgatgt
     1141 gcgagtttca aaaaccttca acctcaaaga caccgcttcg attgaagtgc tggccgaagt
     1201 ctttaacctg tttcgggctg acaacctggg ctcaaacggc acgacgaacg atggtttttc
     1261 agtcgttcgg cgcgacacac cagccggacc gaattcggtg ttgaattcgg ctggttcgcc
     1321 acagcaaatg cagcttggcg tgagattccg gttctaagga attctctctt gattacattg
     1381 ctataattca ctcaatggtg tataaccaat gtatgaacaa tcgaaagacg ccaagtggtt
     1441 tccggctttc tgaaaaggcc aaactcttat tacttgcact ctcccagaaa ttgggaataa
     1501 gccagactgc ggtgattgaa atagccgttc gccagatggc ggaaaccgaa catattgaat
     1561 tggaaaaagt gaacgttcgc gagggactgg atgagacaat tatcacttct aaatcttgat
     1621 caaccctctg tgaatggccg ttgtgagcca ggggaagtca ttcactcgga tgctttaaca
     1681 gcgcttgctc aaattcctgc cgatacgttt cgttgcgctg tcaccagccc tccatattgg
//
LOCUS       DAOQDO010001574         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73257_length_1738_cov_3.163993, whole genome shotgun sequence.
ACCESSION   DAOQDO010001574 DAOQDO010000000
VERSION     DAOQDO010001574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73257_length_1738_cov_3.163993"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PKZ53_20840"
     CDS             <1..164
                     /locus_tag="PKZ53_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNJ42943.1"
                     /translation="YLLQIFTKPIQDRPTFFFEIIQRMGSESFGKGNFKALFESIERD
                     QAARGTLDK"
     gene            complement(236..1264)
                     /locus_tag="PKZ53_20845"
     CDS             complement(236..1264)
                     /locus_tag="PKZ53_20845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ42944.1"
                     /translation="MTQTAPRRVLFVDDEPAMTQLGKVVLERAGHRCSLATNGEEGLQ
                     RILTDHPDLVILDYMMPGMSGAEVFRALRTNSTFKDVRETPVLMLTAKTDNETERRQL
                     MELGLAAYLTKPFGHRELLNVIDNILITSEVRRQHLQTAADIKSMYIGAIHTLLKLLE
                     TKDPYAQEHSQMAELVSVAVAEQYGLNEYQIETIRMAALLHDIGKIEIPEHILNKPEP
                     FSADERSVMQQHVTHSVQILDAIPEMREVAILVGCHHERIDGKGYPQRLDADEIPLGA
                     RIIAVADAYDAMQGHRSYRTRLSKEETIAQLRAHAGTQFDGDVVEYFIEALETIEARK
                     SDKVTSDR"
     gene            complement(1454..>1738)
                     /locus_tag="PKZ53_20850"
     CDS             complement(1454..>1738)
                     /locus_tag="PKZ53_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42945.1"
                     /translation="AELETTRHELDQTRMQLEQSRAELFAAMNVGRSAPPPDQVNAFR
                     NSLMMVLGFSELLNRGEYGQMNPQQFEMFRNIEHHAKQMSALLETLLPSS"
BASE COUNT          430 a          440 c          414 g          454 t
ORIGIN      
        1 gctatttgtt gcagatcttt accaaaccaa tccaggatcg gccaaccttc ttctttgaaa
       61 tcatccaacg tatgggcagc gagagctttg gaaaaggcaa tttcaaagcg ctgtttgaat
      121 caatcgaacg cgaccaggcc gcccgtggca cgttggacaa atagaaacta cccacaaaat
      181 gaccaatgac agggtgataa ctgacaaaat ctcttgatgg tcactcatca ccttgtcacc
      241 tgtcacttgt caccttgtca ctctttcgag cttcgattgt ctctagagct tcaataaagt
      301 actcaaccac atcgccatca aactgagttc cggcatgggc tctgagctga gcaatggttt
      361 cttctttcga taaccgggtg cgatacgaac gatggccctg catagcatca taggcatcag
      421 ccaccgcaat aatccgcgcc ccaagcggaa tttcatccgc atccaatctt tgcggatacc
      481 ccttcccgtc aatccgctcg tgatggcatc ccactaaaat cgcaacctcc cgcatttctg
      541 gaatagcgtc taaaatctga acgctatggg tcacgtgttg ctgcataaca gatcgctcat
      601 cggctgaaaa gggctcgggt ttgttgagga tgtgttcggg aatctcaatt ttcccaatat
      661 cgtgaagcag cgcggccatc cgaatggttt caatctggta ctcatttaac ccatattgct
      721 cagcaacggc aactgaaacc agctcagcca tttgagagtg ctcttgggca tagggatcct
      781 ttgtttcgag cagtttcagg agggtatgga tcgccccaat atacatggac ttgatatcag
      841 cggcggtttg caggtgctgc cggcggactt cgctggtaat gagaatgttg tcaatcacat
      901 tgagtaattc gcgatgacca aacggtttgg ttaagtacgc cgccagccca agttccatca
      961 actgacgtcg ctcggtctca ttatcggttt ttgccgtcag catcaggacg ggagtttccc
     1021 gcacatcttt gaatgtagag ttggtgcgaa gtgcccgaaa aacttcagct ccactcatac
     1081 caggcatcat gtagtccaaa atgaccaggt ctgggtgatc cgtcaaaata cgttgcaacc
     1141 cttcttctcc atttgtggcc aaagaacagc gatgtccagc tcgttccaaa acaactttgc
     1201 cgagctgggt catagctggt tcatcatcaa cgaagaggac tcttcgtgga gcagtttgcg
     1261 tcatagcgaa atttccattt tctgtgtgga atgagttttt ggtgtgagat ccttagactt
     1321 cgagcttggg actaaagaaa aggggcttgg ggctgaagaa aacgggcctg gggctgaaca
     1381 cattgggcta aagaagacat ctggctcaaa gtattgaacc cattttcttc agccccaagc
     1441 cctgagcccg tgcttatgag gatggcagca atgtttccaa cagtgctgac atttgtttgg
     1501 cgtgatgttc aatattgcgg aacatttcaa attgttgagg gttcatttgc ccatattcac
     1561 cccgattcaa cagttctgag aaccccaaca ccatcatcag tgaattccga aacgcgttga
     1621 cctgatctgg cggcggggca ctgcgcccaa cattcatcgc cgcaaagagt tcggcccgtg
     1681 actgttccag ttgcattcgg gtttgatcca attcgtggcg ggtggtttca agttcagc
//
LOCUS       DAOQDO010001575         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73325_length_1737_cov_3.969679, whole genome shotgun sequence.
ACCESSION   DAOQDO010001575 DAOQDO010000000
VERSION     DAOQDO010001575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73325_length_1737_cov_3.969679"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            721..1647
                     /locus_tag="PKZ53_20855"
     CDS             721..1647
                     /locus_tag="PKZ53_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42946.1"
                     /translation="MKSTLIQKILYTVLVGLVVGNLVTPAALAQTNTSTRLKTVTVTV
                     NTEGNATIDPKDFMVYEDKIQQTVVSAIPASSEATPLNLAIVMETALPQINVELQRLR
                     TFVRELPAGSRVMVGYVWGNYLTVQQPFSDDLDAVAASMHVVPATQNGVWSPYLPLMD
                     VLKKFNGLPEGRNEVLFISNGFDPFNGSFSSPSSNLYLDRAVRQAQRSNITIHTVFSP
                     APYVRRFNAVLAGQSALAYVAEETGGQAYIGTRTGFVTFDSPLKNLSNALNNQYVITY
                     QSTNLDKKFHKVEVRTDFSNVKVSVLTGYSVK"
BASE COUNT          471 a          412 c          427 g          427 t
ORIGIN      
        1 gtgcttggtg tttagtgctt ggtgcttggt gtttagtgct tggtgcttgg tgcttagctc
       61 tcaatagtca cgacatcgtg cgcgaaattt tggctcgatt ctagccgggc aaccaatcac
      121 aacatcgtgc gcaatgacgc agttacactt gtctagcaaa tcgaaattct gttccatgtt
      181 ttttgttcac caaatggcaa gtgccgaggg gccgaatctt ttcatcatgg tcatttttgg
      241 atttggggac aagggttgaa actgcgcacg atgccgtgag tgcttggttt gactagagtg
      301 cgcacgatgt tgtgactgcg ttcgtgcgca cgatgtcgtg actattgaga cttagcgctt
      361 aggacccatt accttccaag aaccaagaac caagaaccaa gaaccaagaa ccaagcacca
      421 agcatcaaga accaggaacc agccaccttt tgagtcattt tcaccgtggc agttgagcaa
      481 atgaggcaaa attgcacagt cgtgaaaacc ttcccggacc gggaaggttt ttttgtgttc
      541 ggaaaactcc tggaacatga gcagaaccaa aggtttagaa agtccgtagg acggaagtaa
      601 gcaatcactt actttggcat aaaggttgct ctcttaattc tcagagagca caaaccacaa
      661 ccacgtgagc ccgatggcaa agcggaggta gtggtaattc ggcaacagga ggcgacacat
      721 atgaaatcaa cacttattca aaaaattctt tacacggtgc tggtcggatt ggtggttggc
      781 aatttggtaa ccccagcggc actggcccaa accaatacca gcacccgatt aaaaaccgtt
      841 accgtcaccg taaataccga aggcaatgca actattgatc caaaagactt tatggtctat
      901 gaggataaaa tccagcaaac cgtcgtttca gccatcccag cgtcaagtga agccacgccg
      961 ctcaatctgg cgattgtgat ggaaaccgcc ttaccgcaaa ttaatgttga actgcaacga
     1021 cttcggacgt ttgttcgtga gcttccggct ggttcccgag tcatggtggg ctatgtctgg
     1081 ggcaactacc tgacggtgca gcaaccattt tcagacgatc tggacgccgt tgccgccagc
     1141 atgcacgtgg taccggcaac gcaaaatgga gtctggagcc catacctgcc gttgatggat
     1201 gttctgaaga aattcaacgg attacctgaa ggacgcaatg aggttctgtt catttccaac
     1261 gggtttgatc catttaatgg ttcattttcc tcaccgtcat cgaatctgta tctggatcga
     1321 gccgtgaggc aagcacaacg ttcaaatata acgattcaca cggttttcag cccggcacca
     1381 tatgttcgac gctttaatgc ggtgttagct ggtcaaagtg cactggctta cgttgccgaa
     1441 gaaaccggcg gacaggcata catcgggacc agaaccggtt ttgtgacgtt tgattcacca
     1501 ctgaaaaatc tgagcaatgc gctcaataat cagtatgtga ttacctatca gagcaccaac
     1561 ctcgataaga aattccataa agtcgaggtt agaactgact tttcgaatgt gaaagtgtca
     1621 gtgctgacag ggtattcagt gaagtgaggt tatcaggggc tgggggctga aaagccagta
     1681 accttcgaaa accctgaacc ctgaaccctg aaccctgaac cctgaaccct gaaccct
//
LOCUS       DAOQDO010001576         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73327_length_1737_cov_3.590963, whole genome shotgun sequence.
ACCESSION   DAOQDO010001576 DAOQDO010000000
VERSION     DAOQDO010001576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73327_length_1737_cov_3.590963"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1052)
                     /locus_tag="PKZ53_20860"
     CDS             complement(99..1052)
                     /locus_tag="PKZ53_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ42947.1"
                     /translation="MNDSVWLENLHKSFVLAKGWAFRRTYSEILAVDHISLAVPEGQS
                     VAFIGPNGAGKSTTIKMLTGILRPTSGTARVLGVVPWEQRQKLAFQIGVVFGQRSQLW
                     YHLPPSHTLDLLAKIYNLTPTEYQARRAMLIDRFGLGAFLDTPVRKLSLGQRMRAEVA
                     ASLLHQPKVLFLDEPTIGLDVIARQELRDVIREWNQKEGVTVFLTSHDAGDIEQVAKR
                     VIIINHGRVVLDDKVSSMRRQYLGAKILDVKYQEPPQPFSLPGVTQLKSSGYALKLEI
                     DTQLISIEQVIHEVLKAGSVADIAIEDPPLEEVIAHIYNQE"
     gene            1251..>1737
                     /locus_tag="PKZ53_20865"
     CDS             1251..>1737
                     /locus_tag="PKZ53_20865"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ42948.1"
                     /translation="MLDSVILEATQLGDVAKVRELLQHNPNLVNAKGELGETPLHWAW
                     NREISETTKSNVLNSDDDDAATKYREIASLLIQMGADVNACDEFGKTPLHYSAWKREI
                     AELLITHGADINARDKDGNTALHITAWDNWNDQKTIPELLIKKGADVNVRNLKGSTPL
                     HS"
BASE COUNT          451 a          429 c          433 g          424 t
ORIGIN      
        1 ccaagaacca agaaccaaga accaagaacc aagaaccaag aaccaagaac caagctcttt
       61 ctctattctt cattcttcat tcttcattct tcattcagtt attcctggtt gtagatatgg
      121 gcaatgacct cttcaagtgg agggtcttca atcgcaatgt ccgccactga cccggctttg
      181 agcacctcat ggatgacctg ttcaatcgaa ataagttggg tgtcaatttc cagtttgagc
      241 gcatagccac tgctttttaa ttgggtgact ccggggagtg aaaagggctg gggaggctcc
      301 tgatatttga catcaaggat tttggccccc aggtactgac gccgcatgct tgaaacttta
      361 tcatccagca cgacccgtcc atggttaatg ataatgaccc gtttggcgac ctgttcgata
      421 tctccggcat cgtggctggt gagaaacacc gtaacccctt ctttttggtt ccattcgcga
      481 atgacatccc gcaattcctg ccgtgcaatg acatcaaggc caatggtggg ttcatccagg
      541 aacaagactt ttggctgatg gagcagactg gcggcaactt cagcgcgcat tcgctggcca
      601 agggacaatt ttcgcaccgg agtgtccaga aaggccccca gtccaaaccg gtcaatcagc
      661 atagcgcgac gggcctgata ctcagtcggc gtgaggttgt aaattttggc cagcaggtca
      721 agtgtgtgcg aggggggcag gtgataccag agctgagacc gttgcccaaa aaccacgcca
      781 atctgaaacg ccagcttttg ccgctgttcc cagggcacaa ctcccaggac ccgggcggtt
      841 cctgaagtcg gacgcaaaat cccggtcagc atcttgatgg tggttgattt cccggcgcca
      901 ttggggccga taaacgccac tgactggcct tccggaaccg caaggctgat gtggtcaacg
      961 gccaggattt cactgtacgt ccggcgaaaa gcccagcctt ttgccaaaac aaatgattta
     1021 tgcaaatttt ctagccaaac actgtcgttc atgccttgaa tatcctgaaa attatttgta
     1081 actattctga gacaagattt cgaattcagc gatccttctt gcctgacgct tctttccagc
     1141 cggttcaatc tgtggattgt gtggagaggg tgcaaggctg gtacgattca tagctcctca
     1201 aattgttcgg cgaatcttta taaaatcaag ttaggcaagg gtttatcata atgttggaca
     1261 gcgtgatttt agaggcaact cagttaggcg atgtggcaaa agttcgtgag ttactgcaac
     1321 acaatcccaa tcttgtcaat gccaaaggtg agctgggcga gaccccactg cactgggcct
     1381 ggaatcgtga aatctcggaa acgacaaaat caaatgtgct caactctgat gacgatgatg
     1441 ctgccaccaa ataccgtgaa attgccagcc tgctgattca aatgggagcg gacgtcaacg
     1501 cctgtgatga gtttgggaag acgccgcttc attattcagc ctggaaacgt gaaatcgctg
     1561 agttgttgat tactcatgga gctgacatca atgctcggga taaggatgga aacacagccc
     1621 tgcatatcac ggcctgggat aactggaacg atcaaaaaac catcccggag ttgctgatta
     1681 aaaaaggtgc agatgtcaat gtccgcaatc tgaagggcag cacaccatta cattcgg
//
LOCUS       DAOQDO010001577         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73337_length_1737_cov_2.936980, whole genome shotgun sequence.
ACCESSION   DAOQDO010001577 DAOQDO010000000
VERSION     DAOQDO010001577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73337_length_1737_cov_2.936980"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PKZ53_20870"
     CDS             <1..1072
                     /locus_tag="PKZ53_20870"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ42949.1"
                     /translation="VSNHCWRSLLAVERALIFAAALLHDIAKPECTVIDGDNQVSCPG
                     HRRRGMHKARMILWDDVRFQTPSVPFLIREMIVNLIRHLNVPVHWIDQPDPIRTALQA
                     SQSVRCDWLALLAEANVEGQDDSRKLGQLEKIELFRELCRENDCLAHPYAFPSDHSRF
                     VFFRSEPGKLPYTAYDTNQFDVVLLAGIPAAGKDTWVLDFAADRPIISLDDLRLSLDI
                     SPVDNQGPVVVAAKAQAREYLRRQQPFIWNATNITAAVRTQLIDLFAAYQARTTIVYL
                     EAPLQTILQRNAQRTAVVPNRVIYKLAAKLDIPSRTEAHGVHYIVNGTALENWWYQWP
                     IADGSLDSQIVINQSDLDNFEP"
     gene            complement(1165..>1737)
                     /locus_tag="PKZ53_20875"
     CDS             complement(1165..>1737)
                     /locus_tag="PKZ53_20875"
                     /inference="COORDINATES: protein motif:HMM:NF025602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNJ42950.1"
                     /translation="ELGLSLVEPPFHWLTTLPVQYRKRDIQFLSLGDAKAINHPAFFK
                     PADDKCFPAKVYQSGLELADFGYLEEDVPVLVSEPVVWEVEFRCFVHHRTLATFSPYS
                     RFGELACDSNGNWPATADEIAALRQFTDSLLNDWSVSIPEAVVIDVGVISGKGWAVVE
                     ANAAFGSGIYGCDPAQVLPVLAEASCHMSQ"
BASE COUNT          429 a          473 c          432 g          403 t
ORIGIN      
        1 ggtttccaat cactgctggc gatcactgct ggcagttgaa cgtgcgctga tcttcgccgc
       61 cgcgttgctg catgatattg ccaaacctga atgcacggtt attgatgggg acaaccaggt
      121 ttcctgcccg ggccatcgcc ggcggggaat gcacaaggca cgaatgatcc tgtgggatga
      181 cgtgcggttt cagactccct ccgtaccgtt tctcatccgg gaaatgatcg tcaatctgat
      241 tcggcacctc aatgtgccag ttcactggat tgatcagcca gatccaattc ggacagctct
      301 tcaggccagt caaagcgtga ggtgtgactg gctggcctta ctggctgaag ccaacgttga
      361 ggggcaggat gattcccgga agctgggaca gttggaaaaa atagagcttt ttcgagaact
      421 ctgccgtgaa aatgactgcc tggctcatcc ctatgccttt ccttcggacc acagccgatt
      481 tgtctttttt cggagcgaac cgggcaaatt gccatatact gcctatgata ccaaccagtt
      541 tgacgtagta ctgctggctg gcattccggc ggctggaaaa gatacctggg tccttgattt
      601 tgcggcagat cgccccatca tttcacttga tgaccttcgg ttgagccttg atatttcccc
      661 agttgacaat caggggccgg tggtggtggc cgccaaagct caggcacgtg aatacctccg
      721 ccgtcagcaa ccctttatct ggaatgccac caatatcacg gcagccgtgc gaacccagtt
      781 gattgatttg tttgcggcct atcaggcccg gacgaccatt gtttatcttg aagccccgct
      841 ccagaccatt ttgcaacgca atgcccagcg gacggcggtg gtgcccaatc gggtcattta
      901 caaactggcc gcaaagctcg acattcccag tcgaaccgaa gcccatggcg ttcactacat
      961 tgtgaatgga acagctcttg aaaactggtg gtaccagtgg ccgattgctg atggttctct
     1021 cgattcacaa atcgtcatca accagtcaga cctggataac tttgagccat gaaataacaa
     1081 accttgcctg aattggcaga ccaatcgtgt tcgcatcaaa agatagccag gcgcttcaat
     1141 caaaagaact tcacaaatta ctgactactg actcatatga caactcgcct cagctaaaac
     1201 cggcaggacc tgcgccgggt cacagccgta aatccccgat ccgaacgcgg cgtttgcttc
     1261 caccaccgcc cagccttttc ctgaaatcac acccacatca atcaccactg cttcaggaat
     1321 cgaaaccgac caatcattga gaagcgaatc agtaaactgg cgtagcgcgg caatttcatc
     1381 agcggttgcg ggccagttgc catttgagtc acaggccagt tctccaaatc gggagtaggg
     1441 ggaaaaggtt gcaagcgttc gatgatggac aaaacatcga aattcgactt cccacacaac
     1501 tggttcagaa accagtaccg ggacatcctc ctctaagtag ccaaaatcag ctaactccag
     1561 acctgattga tatacctttg cgggaaaaca tttgtcatct gcaggcttga aaaacgccgg
     1621 gtggttgatc gctttggcat caccaagtga caggaattga atgtcgcgtt tgcgatattg
     1681 aaccggaagc gtcgtcagcc agtgaaatgg cggttcgacc agagacaggc ccagttc
//
LOCUS       DAOQDO010001578         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73354_length_1737_cov_2.508918, whole genome shotgun sequence.
ACCESSION   DAOQDO010001578 DAOQDO010000000
VERSION     DAOQDO010001578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73354_length_1737_cov_2.508918"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..288)
                     /locus_tag="PKZ53_20880"
                     /pseudo
     CDS             complement(55..288)
                     /locus_tag="PKZ53_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(285..542)
                     /locus_tag="PKZ53_20885"
     CDS             complement(285..542)
                     /locus_tag="PKZ53_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNJ42951.1"
                     /translation="MSTVKTAFSISKTLLTATDEAARELNLPRSQVIALALEEFLCKH
                     RNQQLLTQLNAAYGDDAFEEEHEVQTRLKPQFRAILENDEQ"
     gene            763..>1737
                     /locus_tag="PKZ53_20890"
     CDS             763..>1737
                     /locus_tag="PKZ53_20890"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ42952.1"
                     /translation="MQKLHRLCLTAGIGVLIGIYAFCWHCQTIHSSFSTPALVSSEAD
                     HPLELNYRVREIQPPPLQTEPVWPPVEQLDPVRTRHLLDRLPPERPLPPETESVVLPE
                     PAMPLPVSATVVKTEFSPGTKLEPPDASPTGPLEVQRVSPKGAVEFSRSLTVHFSQPM
                     VALSGLADMHTVQSVVKLDPQPPGHWRWVGTQTLIFEPEAGRFPMATLFQATIPAGVR
                     SVAGHELQQPVEWAFQTPPLRVKTSFPEQAAASLQPLLVLEFDQRIDPTEVLHSLSLE
                     AGKQRFAIQVATPEQLAQDQDVSQRVESATPGRWIVFQPVNRLPADATI"
BASE COUNT          394 a          468 c          431 g          444 t
ORIGIN      
        1 tgttttccgt gttttccgtg gttaaaatgt ctggaaattt tcggtaaggt acttattcac
       61 cacactggct tcagtcagat ttccctctcc aacatttaaa agaacattgc caggagctat
      121 cgctcgtttg agattggttg ttaaggcaca cacaactgtt gttcgaagcc tgctcttatt
      181 aaagatgtta ttttgaacca cgatacaggg ccggcgatac ccaggttctg agccagccgg
      241 ctcatcgaat tcaatccaat agatttcacc ttgcagcacg gttctcattg ttcatcattc
      301 tccaggattg cacgaaattg aggtttcagg cgggtctgaa cttcgtgctc ttcctcaaac
      361 gcatcgtcac cataggcggc attgagttgg gtcaggagtt gttgatttcg atgtttacag
      421 aggaattcct caagggccag cgcaatgacc tggcttcgag gaaggttgag ttcccgtgcg
      481 gcttcgtcgg tggcagtgag cagtgtcttt gaaattgaga acgctgtttt tacggtagac
      541 atacaaagta ttcctctcgg gtatacctgt gagtatagca aacagcgtga tacgccttct
      601 atgttgaaat tcgctgagaa atcctggtct tagaactcaa aagtgtcaac gcgttgacaa
      661 ccacgtaaag aattgaattc acttgagtta agtggggaaa ctcagttgag catcttttca
      721 acgggtatgt cccaaactgg tagtatttca ggagttgcca tcgtgcagaa attacatcgc
      781 ctgtgtctga cggctgggat tggagtcctg attggaatct atgccttctg ctggcattgc
      841 cagacaatcc attcttcttt ttcaactccg gctctggtct catcagaggc agaccacccg
      901 ctggaactca actatcgagt tcgggaaatc caaccgccgc cgctccagac tgagccggtt
      961 tggcctccgg ttgaacaact tgatccggtt cgcaccagac acctccttga ccggttaccg
     1021 ccggaaagac cactcccgcc ggaaacagag tcggtcgttc ttccagaacc agcgatgccg
     1081 ctcccggtgt cagccaccgt cgtcaagact gaattttcac ctggaacaaa gcttgagccg
     1141 ccagacgcct ctccaactgg accgctcgaa gtccagcgtg tctcgccaaa gggagcggtt
     1201 gaattttccc gctcactcac ggttcatttt tcacagccga tggtggcgct ctcgggactg
     1261 gctgacatgc acactgtcca gtcagtcgtg aaacttgatc cgcagcctcc ggggcactgg
     1321 cggtgggttg gcacccaaac actgatcttt gaacctgaag ccgggcgatt tccgatggca
     1381 actctttttc aagcgacgat cccggcggga gtccgttcgg ttgccggcca tgaattgcaa
     1441 caacctgtcg aatgggcctt tcaaacaccg ccgctccggg tgaaaacttc atttccggaa
     1501 caggccgccg cgtcactcca gccgctcctg gtgcttgaat ttgaccagcg aattgatcca
     1561 actgaggttc tgcactcgct gtcactggaa gccgggaaac aacgctttgc catccaggtg
     1621 gcaactcccg aacaactggc tcaggatcag gatgtttccc aacgggttga atcagccaca
     1681 ccgggacgct ggattgtctt tcagcctgtc aaccgacttc cggctgatgc aacaatc
//
LOCUS       DAOQDO010001579         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73407_length_1736_cov_3.678763, whole genome shotgun sequence.
ACCESSION   DAOQDO010001579 DAOQDO010000000
VERSION     DAOQDO010001579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73407_length_1736_cov_3.678763"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..>1736
                     /locus_tag="PKZ53_20895"
     CDS             477..>1736
                     /locus_tag="PKZ53_20895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ42953.1"
                     /translation="MKICPACNHQFEDTLKFCPNDGTALASPDTVPSTLPNNPKETDS
                     LAGKLIAGRYQILARLGEGGMGAVYKAEQTMMNRTCAIKIMNPAYTQDHDAVSRFHRE
                     AQMSSKLDHPHAIHIYDFGNTEDGLLYLVMEFVEGQTLSSLLKQSGPLSLERTMTIAR
                     QAAAALEAAHKLNIVHRDLKPDNIMIAHRDNSDWVKVLDFGIAKVSGEDHRWDVTKAG
                     MVIGTPLYMSPEQLSGDRLDPRSDIYSFALIIYQMLTGGLPFTGDNAQAVMVKRLTET
                     PLPMATVNPRVSISATVENAIMQGLARNRDHRPYDVGTFLQLLEQAIRTTSPSAFAGT
                     IQYVAQRGDTNPISPAATHVTGAKGPSAPASSSVPASSPTPPTPGQPQRPVQPIGSNS
                     AMPPIPTIPQPQGTPGQAFGAPPVPPGF"
BASE COUNT          454 a          485 c          390 g          407 t
ORIGIN      
        1 actgaagcag aggtactttc cggtgccaaa cgagaagtga attggaatga agaattgaga
       61 atgaagaatt gagagtgaag aatgaagaac gatatcgttt tttagctaac tatttctgta
      121 gtgttttcac tgatcacagc tcagaaacta ttgaaaaatc tggatttgtt tttcgtgttt
      181 ttcgtgtatt gcgtggtttc ttgatctgaa attctatcgt tacgctccgc cactgacacc
      241 caattcctga aagcttgcat ccagccgctt gactccgtac actctcaact tctcaataac
      301 tacttcgtct agctgcaact tattgttttt gaatcagttg taccgttttg gaataaaatc
      361 cttatattca aaaacaatca agtaattcaa tcaaataaac ttagtgaacg actgacgact
      421 gacgactgac tggtattatt tgacacattc gtgcttttca atattgaagg ttcgctatga
      481 aaatctgtcc tgcctgtaat catcaattcg aagacaccct taaattctgc cccaatgacg
      541 gaacggcgct ggcctcacca gatacggttc cttcaaccct accaaataac ccgaaggaaa
      601 ctgattccct ggctggcaaa ttgattgccg ggcgctatca aatcctggcc aggcttggcg
      661 aaggcggaat gggtgcggtc tacaaagctg agcagacgat gatgaatcga acctgcgcca
      721 tcaaaatcat gaatccggct tacacccagg atcacgatgc cgtcagccgt tttcatcgcg
      781 aagcgcagat gtcgagcaag cttgatcatc cgcatgccat tcatatttat gactttggaa
      841 atacagaaga tgggctgctc tatctggtca tggaatttgt cgaaggccag accctgtcct
      901 cactgctgaa acaaagcggt ccgctttccc ttgaacgcac catgacgatt gcccgtcagg
      961 ccgccgccgc actcgaagcc gcccacaagc tcaacatcgt gcaccgggac ctcaaacctg
     1021 acaacatcat gattgcccac cgcgacaaca gcgactgggt caaggtcctg gattttggaa
     1081 ttgccaaagt atctggtgaa gaccaccgct gggatgtcac caaagctgga atggtgattg
     1141 gaacgccgct ttatatgtca ccggaacaac tttccggaga tcgcctggac ccacgctcag
     1201 acatctatag ctttgcactg attatctatc agatgctcac tggcggactg ccttttacag
     1261 gtgacaatgc ccaggccgtt atggtcaaac gactgaccga aacacctctt ccaatggcga
     1321 cggtcaatcc acgagtcagt atttcagcca cggttgaaaa tgcgattatg caaggactgg
     1381 cacgcaatcg tgatcaccgt ccctatgacg tcggcacgtt cctccaactg ctcgaacagg
     1441 ccatccgaac cacatcgccc tcagcctttg ccggaacgat tcaatatgtg gcgcaacgcg
     1501 gcgacaccaa tccaatcagc ccggcggcaa cccacgtgac cggggccaaa gggccgtcgg
     1561 caccggcttc cagttcagtc ccagcatcca gccctacccc accaacccca gggcaaccgc
     1621 agcggccggt tcagccaatc ggatccaaca gcgcaatgcc cccgattcca accattcctc
     1681 agccccaggg aacaccaggg caggcatttg gggcgccgcc cgttccaccg ggattt
//
LOCUS       DAOQDO010001580         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73421_length_1736_cov_3.013682, whole genome shotgun sequence.
ACCESSION   DAOQDO010001580 DAOQDO010000000
VERSION     DAOQDO010001580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73421_length_1736_cov_3.013682"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKZ53_20900"
     CDS             <1..181
                     /locus_tag="PKZ53_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42954.1"
                     /translation="ESAVSGQWFAKFISLNDLIVFHPAMSVQTSMAGFSLVLIVSISQ
                     NDSIFPGKLSYSQPQ"
     gene            282..1184
                     /locus_tag="PKZ53_20905"
     CDS             282..1184
                     /locus_tag="PKZ53_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42955.1"
                     /translation="MSFFRLQLYCFVILLALGSAAIPNFSSLQAQSKSVRAINSIDCD
                     VLPEPVFQKIPPGTVTPKKSPITLKQLSTSRNHITDGGDWLKNNQLSVPVYRVPNPNL
                     NDPGNTPDIIPARYLDFLLVKAIRSSNLTFLIYGEHYADGTFFFAVNEKRGTVEHAYD
                     FGQFSYPRGLNQKEEAKMPVVWAVQEGKILYVSYFHMGYAKVSNGMNGYLSAIDTSKN
                     KILWSSKPQVCNSQTFEIVGDVIISGYGFTAEPDYLYLLDKRSGEILQQIKLKTAPEL
                     ILRKNNLLYVRCYDTDYLFEIAGL"
     gene            1358..>1736
                     /locus_tag="PKZ53_20910"
     CDS             1358..>1736
                     /locus_tag="PKZ53_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42956.1"
                     /translation="MNIPTFTVIDRTLCLTTPECRVQSFELDQLKDIGFLSLSTDRTS
                     AVFIRAFITPDQQFLVADIRQQSTTPTQAFERLWQAHFRRNFAIVGMSESEYQEFAPL
                     AETVFSQCFQQIAQLTQAAQSGKS"
BASE COUNT          464 a          433 c          364 g          475 t
ORIGIN      
        1 ggagtcagca gtcagcggcc agtggttcgc taagtttatt tcgttgaatg acttgatcgt
       61 ttttcatcca gcaatgtcag tgcaaacaag tatggccggt ttttctttag tcctgattgt
      121 ttcgatcagt cagaacgact cgatatttcc aggcaagttg tcctattctc agccccaatg
      181 aaggttctgg gttatgaaaa acaaaaaaac agccgcttac atcatcagag caagactgtt
      241 gtttcttatt tttgcctcgt ttgatcagga ggatcaattt catgtcgttt tttcgacttc
      301 aactttattg tttcgtcatt ctgctggcgc ttgggagtgc tgcaattcca aacttttcca
      361 gtttgcaggc gcaatcgaaa tcagttcggg caatcaattc aattgattgc gacgttttgc
      421 ctgaacctgt cttccaaaaa atccctcccg gcaccgtaac tcccaaaaaa tcgcccatta
      481 cactcaaaca actctcaacc agccgaaatc acatcacaga tggaggagac tggctcaaaa
      541 acaatcaact ttcggtaccg gtctatcggg ttcccaatcc aaatctcaat gaccctggaa
      601 atactcccga catcattcca gcccgctatc tggattttct gctggtcaag gccatccggt
      661 cttcaaacct gacctttctg atctatggtg aacattatgc cgatggaacc tttttctttg
      721 ccgtgaatga aaagcgtggc accgtcgaac acgcctatga ttttggacaa ttttcctatc
      781 ctcgcggcct caaccaaaaa gaagaagcca aaatgccggt ggtgtgggca gtccaggaag
      841 ggaaaatcct gtatgtgtcg tacttccaca tgggctatgc caaagtgtca aatggaatga
      901 atggctatct gagtgcgatt gatacgagca agaataaaat cctctggagc agcaaacccc
      961 aggtatgtaa ttcgcaaacg tttgaaattg ttggagatgt tattatcagc ggatatgggt
     1021 ttacggcaga acccgattac ctgtacctgc tcgataaacg ctcaggtgaa atcctccagc
     1081 aaatcaaact caaaaccgcc cctgaactga tcctgcgcaa aaacaatttg ctgtatgtcc
     1141 ggtgctatga caccgattat ctctttgaaa tagctggact ttaagtttag gactgaagac
     1201 attgggctga agaaaccggg ctgaagaaaa cgggctgaag aaaatgggct gaagaagtgg
     1261 ttttacttca tccctttggt tgccctgagc ttgcgaatct tcagcccaaa ttcttcaacc
     1321 ctcaacccca agcccgatgt ctttcacccg gaaccccatg aatatcccca ccttcaccgt
     1381 cattgatcga acactctgtc tcacaactcc cgaatgtcgc gtccagtcct ttgaacttga
     1441 ccagttaaaa gacatcggat ttctttcatt gagcactgat cgaacctcag cggtcttcat
     1501 tcgggcattt ataacgcctg accagcaatt tctggtggcc gatattcggc agcaatcaac
     1561 cacgccaacc caggcttttg aacgactgtg gcaggcccat tttcgtcgaa attttgcgat
     1621 tgtgggaatg tctgaatcgg agtatcagga atttgccccg ctggctgaaa cagtcttttc
     1681 tcaatgtttt caacaaattg cccaattgac tcaggcagct caatcaggaa agtcat
//
LOCUS       DAOQDO010001581         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73506_length_1735_cov_2.689286, whole genome shotgun sequence.
ACCESSION   DAOQDO010001581 DAOQDO010000000
VERSION     DAOQDO010001581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73506_length_1735_cov_2.689286"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKZ53_20915"
     CDS             <1..475
                     /locus_tag="PKZ53_20915"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42957.1"
                     /translation="YDAGFTVMRQYEAVDKADDVKRISATEWEIKAGARVRVKVTMVA
                     PTRRYHVALVDPLPAGLEILNPALATTGSLPPEQSDVTQSWNWRWARTWFEHQNLRDD
                     RAEAFTSLLWDGVYSYVYYAQATTPGRFIVPPTKAEEMYAPETFGRGQTDVVTVR"
     gene            659..1102
                     /locus_tag="PKZ53_20920"
     CDS             659..1102
                     /locus_tag="PKZ53_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42958.1"
                     /translation="MNSPGADENSTVEIETEPSLEDLLAQTEEAYHRCYVAFDETWKE
                     KTDEAHDILARERERYHRLIQKLNQRARGIRIKLAWLVRESLKSGETAPFQEAQALFQ
                     QVPPVLQRWLLATQFTEAQIRSDDQTLAEHLDAARTRRWKKFGVL"
BASE COUNT          442 a          440 c          468 g          385 t
ORIGIN      
        1 gtatgacgcc gggtttacgg tgatgcgcca atacgaagcg gtggacaaag ccgatgacgt
       61 gaagcgcatt tccgcaaccg aatgggaaat caaagccggt gccagggttc gagtcaaagt
      121 gacgatggtg gcgccaaccc gccgctatca tgtggcactg gtggatccgc ttccggcggg
      181 ccttgaaatc ctcaacccgg cgctggcgac cacgggtagt ttgcctcctg agcaaagtga
      241 cgtgacccaa tcgtggaact ggcgctgggc cagaacgtgg tttgaacacc aaaacctgcg
      301 cgatgaccgg gccgaagcgt tcacgtcgct gctgtgggac ggcgtgtata gctatgtcta
      361 ttatgcccag gcgacgactc cggggagatt tatcgtcccg cccaccaaag ccgaagaaat
      421 gtacgctccc gaaacctttg gtcggggtca gacagatgtg gtgacggtgc ggtgatccgg
      481 tccgggttcc gggttccggg ttccgggttt ttgaagaggg cgcggttcaa agcaacagcg
      541 cccttccttc attctgtcat cctgtcattt gtcttcttgc cagtgggcag gccgtgaacg
      601 acctgcctca agtcaaccac cgctacgcgg cgaaaacgag attgctgacg caaaaccaat
      661 gaactcaccc ggtgctgatg aaaattcaac cgttgaaata gagactgaac cttcgcttga
      721 ggaccttctg gcgcaaaccg aagaagccta tcatcggtgc tatgtcgcct ttgatgaaac
      781 ctggaaggaa aaaacggatg aagcccacga cattctggct cgggaacgag agcgttacca
      841 caggctgatt caaaagctga atcaacgagc cagaggcatc agaataaagc tcgcctggct
      901 ggttcgcgaa agtctaaaaa gcggagaaac tgctccgttt caggaagccc aggccctgtt
      961 tcaacaggtg ccgcctgtcc tgcagcgctg gctcctggcg actcagttta cagaagctca
     1021 gatacgttct gacgaccaga cacttgcgga gcatctggat gcggctcgaa cgcgacgctg
     1081 gaaaaaattt ggagtgctgt gacttgtcac agctttggta tccagcgact tgtcgctgat
     1141 tgctgattgc tgattgattt tattccactg gcgataagct gccgatcttg aaagcgccga
     1201 cttgttggcg cgctccacca tcaggacata gagcgtttct gtgacaatct ggggagtcat
     1261 cccggtaaca cataacaaca cttttttgcg ctttgacatc agtaacacct ttcatcaacc
     1321 aaatcacggt ggagcaaagc gtacctgaaa aaaatggtca tcttcgaact ggcaacattg
     1381 ctggaaccag tcttcaacca aaaagaactc gtgatggttc agggtaatgc catcaagtgt
     1441 gagacgtaac gcaggggaca aggcagaaaa cctctcgtca aaaggccata ctctgaacct
     1501 ctttctaaac tgggctctat ttgatgtaaa cgcgtgctgg ctgagggaaa gagcacataa
     1561 ggacaggttg gggaacccgt atcaggataa agctgggaaa atatgcgctt ttgtactcgc
     1621 aaatgtcaat taccggtaaa caaaaaccag aactggcttt caaaaagggt gaattccatt
     1681 cttgtttttt aggggtgagt caagcatact ttgcgccatc ctttcagact tcacc
//
LOCUS       DAOQDO010001582         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73519_length_1735_cov_2.278571, whole genome shotgun sequence.
ACCESSION   DAOQDO010001582 DAOQDO010000000
VERSION     DAOQDO010001582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73519_length_1735_cov_2.278571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..655
                     /locus_tag="PKZ53_20925"
     CDS             188..655
                     /locus_tag="PKZ53_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42959.1"
                     /translation="MLRNRGYQILLWCFVVFAIVGLPACGKSKAATPPKPVTFAEAQK
                     LKDTLVSVEGYLGLGNQVSVNKNGSQGHCTIQFYRDETRQGTPINIHINLGNAPNQME
                     MLPQNYTVKDLKIRGKDGKLVDYNQKVRVTGKTGLPLNIAWISVESIEQFPKG"
     gene            712..>1735
                     /locus_tag="PKZ53_20930"
     CDS             712..>1735
                     /locus_tag="PKZ53_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42960.1"
                     /translation="MISFSRRVPVIGFGLLLVAGLFFSTSTLAATPKKDVMYLVGADE
                     SATKAAAGRLGGTAVGSLTKAFAAAVADLSAGSNVTIKLAAGEYNGDFGSGAYALGEI
                     NAPTATLKLEGGFKPDFSSRSPFRLPSMIVATPQRSAPLLKFAANSKLGAFIVDGVSW
                     DSQASNSYDAKTNSLLYGKSCTFPFVQFYYLETNLLVFQNCVIANSANRAIEPLIRAA
                     TPEAEIRFSNCIIFNNRIPLKLDSARFRNKPARMVVDHCSFLINWAYNPDPETSNPAS
                     LEIGSKDAAGEIVITNNLFYSNFGGAILALGGPPMVVSNNNFVGNGLLHGQSESDAVA
                     MIISAGG"
BASE COUNT          439 a          420 c          458 g          418 t
ORIGIN      
        1 ggcttggggc ttggggcttg ggggaataca ggcgagaata aggaattgaa gcctcaaaaa
       61 acaaggagtt ggggaaacag agacacagag acaccaggac cgggagacag aaagtcctga
      121 ccaaaattcg tccatgcctc cgttgtgatg ttcagtcaag ctgaatttca aaatcggaga
      181 accagccatg ttgagaaacc gtggatatca aattctgttg tggtgttttg ttgttttcgc
      241 gattgtgggc ctgccagcct gtgggaaatc aaaagccgcc acgccgccca aacccgtgac
      301 ttttgctgaa gcccaaaaac tcaaagatac gctggtaagc gtcgaaggct accttgggct
      361 gggaaatcag gtctccgtca acaaaaacgg gagtcagggg cactgcacca ttcagtttta
      421 tcgtgatgaa acccggcagg gcacaccgat caatattcat atcaatttgg gcaatgctcc
      481 caatcagatg gaaatgctgc cgcaaaacta caccgtcaaa gatctgaaaa tcagaggaaa
      541 agacggcaaa ctggtggact acaaccagaa agtaagggtt acggggaaga ccggattgcc
      601 cttaaacatt gcctggatct cggttgaatc aatcgaacag ttccccaaag gataaatttt
      661 ctggaaagtt taatttcaac acagtgtcaa atcaaaatta aaggagcggg tatgatttcg
      721 ttttcacgcc gggtgccggt cattggtttc ggtctgctgc tcgtggctgg tctcttcttt
      781 tcaacctcaa ccctggctgc cacgccgaag aaagacgtga tgtatctggt tggcgcggat
      841 gaatcagcca ccaaagctgc ggctggccgg ctgggaggca cggcggttgg ctcactgacc
      901 aaagcctttg ctgctgctgt ggctgatttg tcagccggga gcaatgtcac catcaaactg
      961 gctgccggag agtacaacgg ggattttggc tcaggcgcct atgccctggg ggagatcaat
     1021 gctccaaccg cgacactcaa actggaaggt gggtttaaac cggatttttc gagtcggtca
     1081 ccgtttcgat tgccttcgat gattgtggcg actccgcaac gaagtgcacc gctgctgaaa
     1141 tttgccgcca attcaaaact gggcgctttt atcgtggatg gcgtcagttg ggattcacag
     1201 gcatccaatt cctatgacgc gaagaccaat agcctgctct atggcaaatc gtgcacgttc
     1261 ccatttgtgc agttttatta tttggaaacc aatctgctgg tgtttcaaaa ctgcgtgatt
     1321 gccaattctg ccaatcgggc gattgaaccc ctgattcgag ccgcgacccc agaagccgaa
     1381 atccggtttt caaactgcat cattttcaac aatcgcattc ctttgaaact cgattcggcc
     1441 cggtttcgca ataaaccggc tcgtatggtc gtggatcact gtagcttttt gatcaactgg
     1501 gcctataacc cggacccgga aacgagcaat ccggccagtc tggaaattgg ctccaaagac
     1561 gctgccggtg aaatcgtcat taccaataac ctgttttatt cgaatttcgg cggtgccatc
     1621 ctggcgctgg gcggcccacc gatggtggtt tccaacaata attttgtcgg aaatgggttg
     1681 ctccatggtc agagtgaatc tgatgccgtg gcgatgatta tttccgctgg aggcc
//
LOCUS       DAOQDO010001583         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73800_length_1731_cov_3.360979, whole genome shotgun sequence.
ACCESSION   DAOQDO010001583 DAOQDO010000000
VERSION     DAOQDO010001583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73800_length_1731_cov_3.360979"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1500)
                     /locus_tag="PKZ53_20935"
                     /pseudo
     CDS             complement(272..1500)
                     /locus_tag="PKZ53_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721642.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
BASE COUNT          405 a          435 c          449 g          442 t
ORIGIN      
        1 agggtatgag cttgagggga aagaaccagc tttcacctgt agttttttgg ttttgcgccg
       61 cgaagcgctg ccgaccggct agccgaactg cagcttttca ggatgctcaa tccaattcac
      121 cacctgcctc gtcccgttgg agcacaattc aaaaaccttc acgtgaaagg tgaaaatatc
      181 gggctaaaga cgttggactg aagactccgg gttcaaacct gaattacccg ttttcttcag
      241 cccattttct tcagcccctg gcccctagcc cctagccctg gtttcgtccc attgggcggg
      301 atggtctggt actgctacgt ctggatgatt gggcttgacg aatagaccga ataaaatcct
      361 ggtcgggact tgtcttcgtc tcggcatagt taaacccttc cagtttacgg cgttcgagct
      421 ttttgcccat ggtgcgttca atcgttctga ccatagcttc atcactcgat gtgaccaggg
      481 tcagggcgtc acccgtttgg gcggcacgtc cggtgcgtcc aatccggtgg gtgtaggcat
      541 cagccgtgtc cggaatgtca tagttaatca cccgtgaaac accagtcacg tcaattcctc
      601 gggcggcgat atcggttgcc accaggatct ggtgacgtcc gcttcgaaac ccatccagcg
      661 ctgcctgacg ctggttttgt gacaaattcc cttgcaatga agcagagcca taccctgatt
      721 ttcggagctg ctcgtcaagg cgcttggccc ggtgcttggt tttggtaaaa accagaaccg
      781 acccttcacg ggtttgccgc agcaattcca tcaggagcgg tgttttgaga tcagaattga
      841 ccgggtaaat cgcgtgagaa accgtagagg ccggtgcaat tttcccaatc tggatgtttt
      901 tcggggattg taaaatttca gttgccagtc ctttgatggc atccggcatg gtcgctgaaa
      961 ataacagcgt ttgacgattt ttcggtaatg tcttcaggat ctgacgaatc ggaacaataa
     1021 aacccatatc aaacatatgg tcggcttcgt cgagaaccag tacttcaaga tgtgataaat
     1081 caatcgtgcc ctgactgata tggtcaagca gccggcctgg gcaggcaacc acgacttcac
     1141 tgtgtttgcg taacttttcc acctggggac gcatggcaac cccgccgtag acggcagtgc
     1201 tccgaatgcc agtttgtcgg ccaagattca caaatgtcgt gtgaatctgt tcggaaagtt
     1261 cacgtgttgg agcaataatc agcgcccgaa cgactccacg ttggcctgtc atcaggcggt
     1321 gaagaatggg caatgcgtac gctgcggttt ccccgttcca gtctgagcca gtcctaaaac
     1381 atcctggcct tgcaaaatca ctggaattga ctggtgttga atcggggttg gaatggtaaa
     1441 accctgggct ttcacccctg aaagtacccg tgggtgcaat tgaaataagt cgaaagtcaa
     1501 agaaaatcct tttcgcaaaa atccggaagc ttattttttt cctgtctcaa tccggaatgt
     1561 gaagaccata caaccaggcg aaatgacagg agattcctgc cttggggtat caaagtgact
     1621 caacgtagca cacttcctga ataaaagcac gggatatagc ctatcacaag attgcagtta
     1681 tggagtgctc cggcgtgacg gagcttttct tatgtgtgga gtgcggtggc t
//
LOCUS       DAOQDO010001584         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73819_length_1731_cov_2.708831, whole genome shotgun sequence.
ACCESSION   DAOQDO010001584 DAOQDO010000000
VERSION     DAOQDO010001584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73819_length_1731_cov_2.708831"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKZ53_20940"
     CDS             <1..569
                     /locus_tag="PKZ53_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42961.1"
                     /translation="PMMAILLIPLLLAAGVGFALAYCAIYTRLIFIPCSIMIEGQNAT
                     SSITRSFQLGKGNTWRLAQIYLFDFAIAIAIFSALGIPLSLYGYFSGALDDPTNLPGW
                     LAIAYSSVDQLSKLLTIPISATAYCLLYFDSRVRQEGYDVELLLSQMPVPVQPPSAQP
                     SPQWGQHLSPGFAGPPHSGPPPGSILGL"
     gene            complement(597..1688)
                     /locus_tag="PKZ53_20945"
     CDS             complement(597..1688)
                     /locus_tag="PKZ53_20945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNJ42962.1"
                     /translation="MTPLEAKLHQRIKTGGEISFRDFMDAALYDEEFGYYTTKPDRIG
                     RKGDYFTSSNVSRFFGELIAKQIWLSWQELGTPQPFSILEFGAGTGRLANDVLTTLET
                     SYPEFFQFLTYVICDRSPVLRQAQHEMLAPFSGKVRWIDQIEPVPAAFVLANEFVDAL
                     SFHRFRLRRGRIETCQVGVRDGKLVPVWTELASPETVQSYLKQTGMPVYDGHEWEICF
                     EAIDWLTAVASSLGCGAILIIDYGDLSDHLSEERHGTARCFFQHRVNTDFFSRIGDQD
                     ITADVNFSALISAGQELGLQTRRLDRQGDFLISLGLLEKLEILCLDVNNTLDSLQERL
                     ALKHFLIPGGLGDHFKVLLQMKLPEKSCS"
BASE COUNT          408 a          480 c          406 g          437 t
ORIGIN      
        1 gtccgatgat ggcaatcctg ttgatcccgc ttctgctggc ggcaggtgtg ggatttgccc
       61 tggcctactg cgccatttat acccgactga ttttcatccc gtgctcgatt atgattgaag
      121 gccagaatgc cacgtcttca atcacgcgta gctttcaact tggaaaaggt aatacctggc
      181 ggcttgccca gatctacttg tttgattttg cgattgcgat tgcgattttt tcagccctgg
      241 ggattccgct cagtttgtat ggatacttca gcggcgcgct cgatgaccca accaatttgc
      301 caggatggct ggcaattgcc tatagctcgg tggaccaact ttctaaattg ttgacgattc
      361 ccatttcagc caccgcctat tgtctgctct attttgatag ccgggttcgt caagaaggct
      421 atgacgtcga attactcttg agccagatgc ccgtgccggt tcaacctcca tctgcccagc
      481 cgtcacctca gtggggacaa cacctttcac ctgggtttgc tggtccacca cattcaggac
      541 ctcccccagg cagcatcctt ggtttgtaaa accgggccct gggtcctgat ttttcttcaa
      601 gaacaactct tttcaggtag tttcatctgc agcaacactt tgaaatggtc gccaagccca
      661 cctggaatca aaaaatgctt gagtgccagc cgctcctgca gcgagtccag cgtgttattg
      721 acgtcaagac agagtatttc gagcttttcg agtaatccca acgaaattaa aaaatcgccc
      781 tgccggtcaa gccgcctggt ttgcagtcca agctcctgtc cagcactaat caacgctgag
      841 aagttaacat cagccgtgat gtcctggtcg cctatccggc tgaaaaagtc ggtattgacc
      901 cgatgttgaa agaaacaccg ggcggtaccg tgtctttctt cggataaatg gtcactcagg
      961 tccccatagt caatgatcag aatcgctccg cagccgagtg aactggccac agcggtcagc
     1021 cagtcaatgg cctcaaagca gatttcccat tcgtgcccgt catacacagg cattccagtt
     1081 tgtttgagat agctctgaac cgtttccggc gatgccagtt ctgtccagac aggaacaagt
     1141 tttccatcgc gcacaccaac ctggcaggtt tcaattcgtc cccggcgcag tcgaaaccga
     1201 tgaaatgata aggcgtcaac aaactcattt gccaaaacaa aagcggctgg gacaggctca
     1261 atctgatcaa tccaccgtac tttcccgctg aacggcgcca gcatctcgtg ctgggcctga
     1321 cgaagcactg gacttcggtc acaaatcaca taggtcagaa attggaaaaa ttcagggtaa
     1381 cttgtttcca acgtcgtcaa gacatcgtta gccaaccgac cggttccagc cccaaactca
     1441 agaatggaaa atggttgcgg agtgcccagt tcctgccagc tcagccatat ttgtttggca
     1501 atcaactccc caaaaaaccg tgacacattg ctgctggtga aataatcacc tttgcggcca
     1561 attcgatcag gttttgtcgt gtagtagcca aactcctcgt catacagagc cgcgtccata
     1621 aagtcgcgaa aggatatttc accgccagtt ttgattcgct gatggagttt ggcttcaagc
     1681 ggcgtcactt gagcacctca atcgcccggt tgagggctgc cctcggcgat a
//
LOCUS       DAOQDO010001585         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73834_length_1731_cov_2.204057, whole genome shotgun sequence.
ACCESSION   DAOQDO010001585 DAOQDO010000000
VERSION     DAOQDO010001585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73834_length_1731_cov_2.204057"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1079)
                     /locus_tag="PKZ53_20950"
     CDS             complement(177..1079)
                     /locus_tag="PKZ53_20950"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ42963.1"
                     /translation="MSRRSLLFIICSVITALAVFCPVQFGQTQGQKGMSVEKAPVSIP
                     PCGTIVSPFIQKEGAKGLFFDPASGIALNANPSSTSSSRSSRQRTSARSQGKGTPASP
                     PTHTSPTLSPDLTDSTTRPATRPENTGLKFWIELIEPTGDTRRVLPTRTFHTGERIRF
                     HFESNVDGYIALTLLEATGTAKTLFPTDQGRAEKSFIEHQKDNILPAEDAWFRFDDQV
                     GTERILVFFSKSHQAIDQLQLQPEMETRSVAVLTQKAQEGSKGIFVEFDGASTPANQV
                     AYFVNPSSDSSAYVVLEICINHER"
     gene            complement(1139..>1731)
                     /locus_tag="PKZ53_20955"
     CDS             complement(1139..>1731)
                     /locus_tag="PKZ53_20955"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ42964.1"
                     /translation="IRTALNAIARNALAEDLVVIFLSSHGSPGKADLVGEGYLVTYDT
                     EIADLYATAFPMRDFTRDVKQRIQAQRKVVFLDTCYSGQANAEGGKGIQMVGELQNQT
                     AQDLCGFGSVVITSSSPHEQSWESPALQNGYFTYFLIQALRQENGYLSIDKVFSFLQD
                     TVPQTVRKEKEATQTPRIMPQPLREAAEIKIGVKTN"
BASE COUNT          440 a          412 c          433 g          446 t
ORIGIN      
        1 ccctcgactt aagtacctta ccgaaaattt ccagacattt taaccacgga aaccacggaa
       61 aaaaatcaac cacgtaccaa acccaatagc tcagaaaatt tatggcaagg tacttaatag
      121 gaggctaaat tattgaactg attgtatttc cataaagccc tgaaccctaa atgggattac
      181 cgttcatggt tgatgcaaat ttccagaaca acataagcac ttgaatcaga agacgggttt
      241 acaaaataag cgacctgatt ggctggcgtt gaagcgccat cgaattcgac aaaaattccc
      301 ttgcttcctt cctgtgcttt ttgagtcagg accgcaaccg accgggtctc catctcaggc
      361 tgaagctgaa gctggtctat agcttgatgc gacttggaaa agaaaaccag gatccgctcg
      421 gttccaacct gatcgtcgaa ccgaaaccag gcatcttccg ccggcaaaat attatctttt
      481 tggtgttcaa tgaaactctt ttcggctcga ccttgatcgg ttggaaagag cgttttggcg
      541 gttccagttg cttccagcaa tgtcagcgca atgtacccat caacattact ttcaaaatga
      601 aaccggatcc gttcaccagt gtgaaacgtt cgggttggga ggacacgcct ggtatctccg
      661 gtgggttcaa ttaattcaat ccagaacttc aaacccgtgt tttctggccg ggttgctggc
      721 cgggtggtcg aatcagtcag atcaggtgac agggttggtg aggtatgagt gggtggtgat
      781 gcgggagttc ctttgccctg agaccgggcg ctagtgcgtt gacgggatga acgggaactg
      841 gatgtggaag atgggttggc gttgagcgct atcccactgg ctggatcgaa aaagagccct
      901 ttggcccctt ccttttggat aaatggggag acaattgttc cacagggagg gattgaaacg
      961 ggggcttttt ccaccgacat gcctttttgc ccctgagttt gaccaaactg gaccgggcaa
     1021 aaaacagcaa gagccgtaat aacgctacag attatgaaca acaggctgcg tcgggacata
     1081 aaaaacttcc tttctggaaa tcaactgtat ggaggttcac cgctaaacca tgaccggact
     1141 aattggtttt gactccgatc ttgatttctg ctgcttctcg cagaggttgt ggcatgatgc
     1201 gtggagtttg ggtcgcttct ttttccttcc gaacggtttg gggaactgtg tcctggagaa
     1261 aactgaacac tttgtcaatt gataaatacc cattttcctg acggagggct tgaatgagaa
     1321 aataggtaaa atacccattt tgtaaggctg ggctttccca ggattgttca tgcggagagc
     1381 tcgatgtgat gactaccgat ccaaatccgc acaaatcctg ggccgtttgg ttttgcaatt
     1441 cacccaccat ctgtattccc ttgccgcctt ccgcattggc ctgcccgctg taacaggtat
     1501 ccagaaacac gactttgcgc tgagcctgga tccgttgttt gacatcccgg gtaaaatccc
     1561 gcatcgggaa ggcggttgca tacaggtcag caatctcggt gtcataggtg accaggtagc
     1621 cttctccaac cagatcggct tttccggggg agccgtggct actcaaaaaa ataaccacta
     1681 aatcctcggc cagcgcattc ctggcaatgg cattcaatgc ggttcgaatg c
//
LOCUS       DAOQDO010001586         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73882_length_1730_cov_2.810746, whole genome shotgun sequence.
ACCESSION   DAOQDO010001586 DAOQDO010000000
VERSION     DAOQDO010001586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73882_length_1730_cov_2.810746"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..>1730)
                     /locus_tag="PKZ53_20960"
     CDS             complement(116..>1730)
                     /locus_tag="PKZ53_20960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ42965.1"
                     /translation="LQEELIERFKEEAAILERLGEQSPHGQIPKLFAYFAEGGNFYLI
                     QEWVEGHTLIERIRKTGRLSESEVVAMLVGLLPVLHYVHSKHIIHRDIKPSNIIFRRQ
                     DHLPVLIDFGIARGVIGAHEEATTRIGTPGYMPLEQAAGRPVLASDLFSLGMTAICAL
                     TGVGPKDLPTDPQTGAVKWQSFVSSVNPGLASVLDRATRMYFTERFASAIEMLQALQA
                     VTVSDGLPVVAGVVPTGLVSEDSAERSFPPASGYATSELQVAALVPPTDQPEAPTIVR
                     AKRIIIPIFNSVVETLTGKGKRKIPLESGPVQPVLPTGRIPSPTLPREIKNRVKMEFL
                     LIRSGTFLMGSPETEPGHGQDEIQCEQVVDRPFYLGAYPVTQEEWKAVMGTPPGGFKG
                     EPRLPVESVSWEEVQEFLKRLNKKEGTTAYRLPTEMEWEYACRAGSTGPYAGTLELMA
                     WYADNSGQRTHPVGQKLPNDWGLYDMHGNVRELCQNQIGTEMTKTYRLSRGGSWADMA
                     SFCRSAQSHVELPECRTNRLGFRVLKEVG"
BASE COUNT          386 a          516 c          417 g          411 t
ORIGIN      
        1 gggttccggg ttccgggttc cgggttccgg gttccgggtt ccgggttttc gaaaatctac
       61 ctttttttca ctttttcgtc cttcttgccc tttaaacccg gaacccggaa cccgactatc
      121 ccacctcttt caacacccga aaccccagcc ggttggtacg gcactctggc aattcgacgt
      181 gtgattgagc cgaacggcaa aacgaagcca tatccgccca actgccaccg cgtgacagac
      241 gataagtttt tgtcatttca gttccgatct ggttctggca caactcacgc acattgccgt
      301 gcatgtcata gagcccccag tcattaggga gtttttgccc aacggggtgg gttcgctgcc
      361 ccgagttatc ggcataccag gccatcagtt ccagcgttcc agcatagggc cctgtactgc
      421 cagcccggca ggcatattcc cattccattt cggttggaag ccgatacgcg gtggtccctt
      481 cctttttgtt gagccgtttc agaaattcct gaacctcttc ccaactcaca ctctcaactg
      541 gcaaccgtgg ctcgcccttg aaccccccag gaggggtgcc catcacagct ttccattctt
      601 cctgtgtcac cgggtaggca cccaggtaaa aaggccggtc aaccacctgt tcacattgga
      661 tttcgtcctg gccatgtcca ggttctgtct caggtgaacc catcagaaac gtgccacttc
      721 gaatgagcaa aaattccatc ttaacccggt tttttatctc tcgtgggagc gtcgggctcg
      781 ggattcgccc agttggaagc actggctgaa ctggtccaga ctcaagtgga atttttcgtt
      841 tccccttgcc agtcaacgtc tcaaccactg agttaaaaat tggaataatg atccgtttgg
      901 cccgcacaat agttggcgct tcgggctggt cggttggcgg taccagagcc gcaacctgta
      961 gctcagaggt cgcgtagcca gacgcgggtg ggaaactccg ttcggcagaa tcttcagaaa
     1021 ccaaaccagt tgggaccacg ccagcgacaa ccggcagacc atctgaaaca gtcactgcct
     1081 gcagagcctg gagcatttca atcgccgaag caaaccgctc agtgaaatac atccgggtcg
     1141 ctcgatccaa aactgatgcc agcccaggat tcactgacga aacaaatgac tgccacttca
     1201 ccgctccggt ttgagggtca gtgggtaaat ctttcggccc aactccggtc aaggcacaaa
     1261 ttgcggtcat tcccaggctg aacaaatcgc tggccagaac cggacgacca gccgcctgct
     1321 ccagcggcat ataccctggc gttccaatcc gcgttgtggc ttcttcgtga gcaccgatga
     1381 ccccacgtgc aatcccaaaa tcaatcagaa ccggcaggtg atcctgccgc cgaaagataa
     1441 tgttgctcgg tttgatgtcg cgatgaatga tgtgtttgga gtgaacatag tgcaggacag
     1501 gcaacaaccc aaccagcatc gccaccactt ctgattcgct caagcgaccg gtttttcgaa
     1561 tccgctcgat cagggtgtga ccttccaccc attcctgaat caggtagaaa ttcccacctt
     1621 cagcaaaata ggcaaacagt ttgggaattt ggccgtgtgg cgactgttcc ccaagtcgtt
     1681 ccaggattgc agcttcttct ttaaaccgct caatcaattc ctcttgcagt
//
LOCUS       DAOQDO010001587         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_73950_length_1729_cov_2.992234, whole genome shotgun sequence.
ACCESSION   DAOQDO010001587 DAOQDO010000000
VERSION     DAOQDO010001587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73950_length_1729_cov_2.992234"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKZ53_20965"
     CDS             complement(<1..603)
                     /locus_tag="PKZ53_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ42966.1"
                     /translation="MNIEIQNLKKYFEKTRAVDDVSFSFTSGQIIGFVGPNGAGKTTT
                     MRIMATMEEPTSGDVLVNGVSVVEEPEKARQLVGYVPDSLFGHTDIAVHEYLDFFARA
                     YGLKGQHRRQVVESVQEFTNLLGIREKMIDSLSKGMKQRVTLARALLHDPMVLIMDEP
                     AAGLDPRARIELRELVKVLSQQGKAIPISSHILTELAEMCT"
     gene            complement(600..>1729)
                     /locus_tag="PKZ53_20970"
     CDS             complement(600..>1729)
                     /locus_tag="PKZ53_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42967.1"
                     /translation="SLQRFVECGGNLLLLGTPVSPLGLVLGQTEQNGLVTHYVGFGQW
                     QTLNNLTLDQITPLQWEKIQDGWLLSHGTFQDTKSVSQANTEFPVVDALAIPVRGLFV
                     MMLIFVIVIGPVNIFLLSRVKRRILLLVTVPVLSLFTCIAISSYAILSEGIRTKNRTE
                     GLTILDETTRLSATIGLTAFYAPLTPGGGLHFGYDTELTPQLGGYSYYSRENSGGGRT
                     IDWSQDQHLASGWITARIPAHFQVRKAELRRERLTITPSNSESYNLVNGLGGNIKQVW
                     FADQSGQMFESGPVPAGAQGTLKRSGKTATAQPDTLRTIYTWNWTGNIWNFTQSPEKV
                     LRPGCYLAWIEASPFIEEGLTGSVARQQQSVVYGITGGSRP"
BASE COUNT          409 a          449 c          466 g          405 t
ORIGIN      
        1 agtacacatt tcagccagtt cggtcaaaat gtgcgagcta atcggaatgg ctttgccttg
       61 ctgggacagg actttgacca gttcgcggag ttcaatccgg gcgcggggat caagcccggc
      121 ggcaggctcg tccataatca ataccattgg atcgtggagc agcgcccgag ccagcgtcac
      181 ccgttgcttc attcctttcg aaagcgaatc aatcattttt tcccgaatcc ccagcaagtt
      241 ggtgaattcc tggacgcttt caaccacctg tcgtcggtgt tgccccttga gcccataggc
      301 acgggcaaag aaatcgaggt actcgtgaac ggcaatatca gtatggccaa agagggaatc
      361 tggaacatag ccgaccagtt ggcgggcttt ttcgggctct tcgacgaccg agactccatt
      421 gaccagcaca tcaccgctgg tgggttcctc catcgtcgcc ataatgcgca tggtcgtggt
      481 ttttcctgcc ccgtttggac caacaaatcc aatgatttgc ccagaggtaa aggaaaacga
      541 gacgtcatca acggccctgg tcttttcaaa atattttttg aggttttgga tttcgatgtt
      601 catgggcggc tgcctccggt aatgccatac accacgcttt gttgctggcg ggctacgctg
      661 ccggtcaatc cttcttcaat aaatgggctg gcttcaatcc aggccagata gcaccctggc
      721 cgcaggactt tttcagggga ttgggtgaaa ttccatatat tgccagtcca gttccaggtg
      781 tagatggtgc gcagggtgtc aggttgagct gtggcggttt ttccagacct tttcaatgtt
      841 ccctgtgcgc cagccggcac tggaccagac tcaaacattt gaccggattg gtcggcaaac
      901 cagacctgtt tgatatttcc gcccaggcca ttgaccagat tgtaactctc tgagttactg
      961 ggagtaatgg tgagtcgttc gcggcggagt tcggcttttc gaacctgaaa atgggctgga
     1021 atccgggcgg tgatccagcc tgaagccagg tgctgatcct gagaccagtc aattgtgcgg
     1081 ccaccgcctg aattttcgcg gctatagtag gaatatccac ctaactgagg tgtcaattcg
     1141 gtatcgtacc caaagtggag acctccaccg ggggtcagcg gggcataaaa ggcggtcagt
     1201 ccaatcgtgg ccgaaagacg ggtcgtttca tcaaggatgg tcaagccttc cgtgcgattt
     1261 ttggtgcgga tgccttctga caaaatggca tacgatgaaa tcgcaataca ggtaaacagc
     1321 gacagtaccg ggacggtcac caggagcagg attcggcgct tgacgcgaga cagtagaaag
     1381 atattgaccg ggccaatcac aatcacaaag atgagcatca tgacaaaaag cccacggact
     1441 ggaatggcaa gcgcatcaac caccggaaac tcggtgttgg cctgacttac tgatttggta
     1501 tcctgaaacg tgccatggga caaaagccat ccatcctgga ttttctccca ctggaggggc
     1561 gtgatctgat ccaacgtcag gttgttgagc gtttgccact gaccaaaccc gacatagtgg
     1621 gtaaccagtc cattttgctc ggtttgaccc aggaccagcc ccagtggaga aactggcgtt
     1681 ccaagcaaca acagatttcc accgcattcg acaaaccgct gaagactcg
//
LOCUS       DAOQDO010001588         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74025_length_1728_cov_3.120741, whole genome shotgun sequence.
ACCESSION   DAOQDO010001588 DAOQDO010000000
VERSION     DAOQDO010001588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74025_length_1728_cov_3.120741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PKZ53_20975"
     CDS             <1..1659
                     /locus_tag="PKZ53_20975"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42968.1"
                     /translation="PFPEEFLKTRNPEPRTRNPVFFLILAVVLLLSSETAFAQKKAVV
                     GKAYSLSYRVGFSDLEAHLFDISIEIGNIREPAIEVAMPAWSPGRYAIYDFAKNVQEF
                     TAQTRSGETLPCGKIDKQTWRIQTRGARDIKVSYRVFGNDLSGTFSVVNRQHANYNGA
                     SIFAYVVNHKPDPVHLIIDPPAGWKVMNGAVDTDGQHEFDFANYDLLIDTPTEISSEL
                     VVRSFEVDGKTYRVAMNQPGGVTGDIDRFVKQVEQIVRAETATMGTPDYKHYTFLMNF
                     DPTGRASDGMEHLNSTQVCVSSTLETPQGRAEALETASHEFFHVWNVKRLRPIELGPW
                     DYSREVHTRSLWIAEGLTSYYGDLFLGRSGVYSPEQMYQLFAGQISYFESLPGRKLMS
                     VEQSSWDTWFIVGAPSRQETSLGRTSVNYYNKGEILGLLLDLEIRGRTNGAKSLDDVF
                     RSMFKAFYDQPAATYYLKGKGYRTEDFLTAVNKVSGADFSDFFERYIRGVEPLEYNGA
                     LKYVGLELVPGRQWSFRDVDQPTPKQLWIRKGWLTGVMSQAVGK"
BASE COUNT          439 a          374 c          483 g          432 t
ORIGIN      
        1 ccttttcccg aagagttttt gaaaacccgg aacccagaac ccagaacccg gaacccggtt
       61 tttttcctca ttcttgctgt tgtgttgctc ctgagttccg aaacggcctt tgcccagaaa
      121 aaagcggttg tcgggaaagc ctattcgtta agctatcgag tcgggttttc tgatctggaa
      181 gcccatctgt ttgatatttc gattgagatt ggaaatatcc gcgagccagc gattgaggtg
      241 gcgatgcctg cctggtcgcc cggacgctat gccatttatg actttgccaa gaacgtccag
      301 gaatttacgg cccagacccg gagcggggaa actcttccct gtggaaaaat agacaagcaa
      361 acctggcgaa tccaaacccg aggtgctcgg gacatcaagg tttcataccg tgtgtttgga
      421 aatgatcttt ccgggacgtt cagcgtcgtg aaccgccagc atgctaatta caacggggct
      481 tcgatctttg cctatgtggt caatcacaag ccggatccgg ttcatctgat tatagatccg
      541 ccagccggat ggaaggtgat gaacggtgcg gttgatacgg atggccagca cgaatttgac
      601 tttgccaatt atgatttact gattgatacc ccgaccgaaa tcagcagcga actggtcgtc
      661 cggtcatttg aagttgatgg caaaacctat cgggttgcga tgaaccagcc aggcggtgta
      721 accggagata tagatcggtt tgtaaagcag gttgaacaga ttgtccgggc tgagacggcg
      781 acgatgggaa ctcctgatta taagcactat acgtttttga tgaactttga cccaactggc
      841 cgggcgagtg atggaatgga acatttgaac tcaactcagg tgtgtgtttc gtcaacgctt
      901 gagacaccgc aggggagagc cgaagcgctt gaaacagctt cgcatgaatt ttttcacgtc
      961 tggaatgtca aacgattacg accaatcgaa cttgggccct gggattacag tcgcgaagtc
     1021 catacccgca gcctctggat tgctgaagga ttgacgagtt attacggtga tttattcctg
     1081 gggcggtctg gtgtttacag cccggaacaa atgtatcaat tatttgccgg gcagatcagt
     1141 tactttgaaa gtttgcctgg aaggaaattg atgtcggttg aacaatcaag ctgggatacg
     1201 tggtttatcg tcggcgctcc gtcacgccag gaaaccagtc ttgggcgaac gtcagtcaat
     1261 tattacaaca aaggtgaaat cctgggattg ctcctggacc ttgagatccg gggccggacc
     1321 aatggcgcca aatcactgga tgatgtgttt cgctcaatgt tcaaagcttt ttatgatcaa
     1381 ccagccgcaa cctattattt aaagggaaaa gggtatcgaa cagaagattt tctgacagcg
     1441 gttaataaag tgagcggtgc tgacttctca gatttcttcg aacgctatat caggggcgtt
     1501 gaaccccttg agtataatgg agctttaaaa tatgtcggtt tggaattggt tccaggccgg
     1561 caatggtcat ttcgagacgt agaccagccg acgccaaagc aactctggat tcgcaaaggc
     1621 tggctgaccg gtgttatgag ccaggcagtt ggaaagtgac gcgtgtagaa aacaggggat
     1681 ggaggatagg ggatggagga taggggatgg aggatagggg atggagga
//
LOCUS       DAOQDO010001589         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74035_length_1728_cov_2.672445, whole genome shotgun sequence.
ACCESSION   DAOQDO010001589 DAOQDO010000000
VERSION     DAOQDO010001589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74035_length_1728_cov_2.672445"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKZ53_20980"
     CDS             <1..238
                     /locus_tag="PKZ53_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42969.1"
                     /translation="MYVLGNEDVLNQATLLGEYETTANRPELPEEERGYRYLDAYLHR
                     TREVTTAEVQRVMRTYLHPDHRTVGYLVNDPKEN"
     gene            389..1702
                     /locus_tag="PKZ53_20985"
     CDS             389..1702
                     /locus_tag="PKZ53_20985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ42970.1"
                     /translation="MDFLEMNANQIAAGPAVKHKGEIERVVLPNGIVLLMAENHTLPM
                     ISMSAMVRFGSRYEAEAQAGLATLLGDMLDEGTPTRTAQEIALATEEVGARLSSFGGY
                     SHSGVGLVCLTEDFPGLLALAAEMLTQSTLPEDRFTMRRERVLAQLKSREDEPRAVAA
                     DAFAELIYGTHPAHRPSLGYPHTVAGLTRDQLAAAYQRFFVPNNSIFAVVGDFSRREL
                     QAQIEAAFANWQPDPAFSLPEVPTVERQTEPREKIIFKEKEQIQVYLGHLGIQRSNPD
                     FYPLLVMDTILGSSPGMTSRIPRILRDEQGLAYTTFSSITSSAGIDPGRFTAYIGTSP
                     ANREKAIRGLRAEIERITTEPVSQEELDIAKSYLTGSFVFNFETNAQVAGFLVEAEVF
                     NLGFDYLETYPEFIRQVTVDDVTRVARQYLDPVNLTTVIVGPVKE"
BASE COUNT          411 a          459 c          445 g          413 t
ORIGIN      
        1 catgtatgtg cttgggaatg aagatgtgct caatcaggcg actttgcttg gcgagtatga
       61 aaccacggcc aatcgcccgg aactccctga agaagaacgt ggctaccgct atctggatgc
      121 gtatctgcac cgcactcgcg aagtcacaac cgcggaagtt caacgggtga tgcgcaccta
      181 tctccacccc gatcaccgca cagttgggta tttggtcaat gacccgaaag agaactaggg
      241 ttcagggttc agggttcagg gttcagggtt tttgaattta tgtccttttt gtcccttatg
      301 ccctttatgt cttttcaaga acctcaaacc tgtcccccaa accctgaacc ccaaagcatg
      361 tcactttgct cacaaaggaa taaatctgat ggattttctg gaaatgaatg ccaaccaaat
      421 cgctgccgga ccggcggtga aacacaaagg cgagattgaa cgggtggttt tgccaaacgg
      481 tatcgtactc ttgatggctg aaaaccatac cctgcccatg atttcgatga gcgccatggt
      541 gcgctttggc agccgatatg aagccgaggc gcaagccggc ctggccaccc tgctgggtga
      601 catgctcgac gaaggcacgc caactcgcac ggctcaggaa atcgcgcttg ccactgaaga
      661 agtcggcgcc cgactgagtt catttggtgg ttattcgcat agtggcgtcg ggctggtgtg
      721 cctgactgaa gattttcctg gattgcttgc cctcgcggca gaaatgctga cccaatccac
      781 ccttccggaa gatcggttta cgatgcgacg cgagcgcgta ctggcccaac tcaaaagccg
      841 tgaagatgaa ccacgtgcag tcgcagcaga tgcatttgct gaattgattt acggaaccca
      901 tccggctcat cgtccatcgc ttggatatcc gcacaccgtg gccggattga ctcgtgatca
      961 actggcagcg gcctatcagc ggtttttcgt ccccaataat tccatctttg ccgtggtcgg
     1021 agatttctcg cggcgtgagt tgcaggcaca aatcgaagcc gcatttgcca actggcagcc
     1081 agacccggcg ttttcattgc cggaagttcc aactgtcgag cgccagactg agccccgcga
     1141 aaagatcatc ttcaaagaaa aagaacaaat ccaggtctat cttggacatc tggggattca
     1201 acgttcaaac cccgattttt atccgttgct ggtcatggac accattttag gcagcagccc
     1261 tgggatgacc tcccggattc cacgcatttt gcgtgatgaa caggggctgg catacacgac
     1321 attttcaagc attaccagtt cagccggaat tgatccggga cgctttacgg cctacatcgg
     1381 cacatcgcct gccaaccgtg aaaaggccat ccgtggactg cgagctgaaa ttgaacgcat
     1441 cacgaccgaa cctgtcagcc aggaagaact cgacatcgcc aaatcttatc tgactggaag
     1501 ctttgtgttt aactttgaga cgaatgccca ggtcgctgga tttctggtcg aagctgaagt
     1561 ctttaatctt ggctttgact atctggaaac ctatccggaa tttattcgcc aggtcacagt
     1621 ggacgatgtc actcgtgttg cgcgccagta tctggatcca gtcaacctca caaccgtcat
     1681 tgttggacca gtgaaggagt agggttcggg gtttttgaaa ggatttaa
//
LOCUS       DAOQDO010001590         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74166_length_1726_cov_3.754638, whole genome shotgun sequence.
ACCESSION   DAOQDO010001590 DAOQDO010000000
VERSION     DAOQDO010001590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74166_length_1726_cov_3.754638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKZ53_20990"
     CDS             <1..289
                     /locus_tag="PKZ53_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42971.1"
                     /translation="VDETPDLAPLSEAPASVIATSEPLQSEPVLDETLHTEPDFETAP
                     TAIPVSQPAIQDLFPPVLAETSPSINQTWDTAETAVPVAPTETKPEGGSIE"
     gene            286..1212
                     /locus_tag="PKZ53_20995"
     CDS             286..1212
                     /locus_tag="PKZ53_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42972.1"
                     /translation="MSRLLGFEVGVLRQLPGETVAIPFGRDGRLGWYLKLSGKRVFSN
                     DLRQSASWQAVGKVENNGYVLSAVEIQQLLDEADAFVQQTQGTPENQRLLGWFAPTQA
                     AWLDGYRKAALTLDDRSLRGLALTLGQRIGEYWRSFDSQTHHLQLPIDDVLSHFLDEL
                     HQVVDNRRENLATNLDAHQFVIQVHADLVYVHLPSPEPLSAQSQQPDGWTEVWITGNP
                     DIWETLTANQRNRFGGQLMTRTKYREVVKGFLKRLRHFPAWVISINQPGPLSTEEMVQ
                     LIEEHRPVEKIYSKDATEFLDGFTQHLIVSRL"
     gene            1248..1622
                     /gene="queD"
                     /locus_tag="PKZ53_21000"
     CDS             1248..1622
                     /gene="queD"
                     /locus_tag="PKZ53_21000"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNJ42973.1"
                     /translation="MEVELSKTFTFEAAHRLPKVPPGHKCARLHGHSFRIEVVVRGPV
                     DPESGWLIDYAEIKDAFEPLFKILDHNYLNEIEGLENPTSENLSRWIWERLSTGENNL
                     ARYLYRVTVAETCTSVCHYFGA"
BASE COUNT          395 a          458 c          445 g          428 t
ORIGIN      
        1 agttgacgaa acgcccgatt tggcccccct gagcgaagca ccggcgtcag tgatagctac
       61 ctctgagccg cttcaatcag aaccagttct ggatgaaaca ctgcacactg aaccagactt
      121 tgaaacggca ccaacggcga ttccggtcag ccagccggcc atccaggatc tgttcccacc
      181 ggttctggct gaaacttcgc catctatcaa tcagacctgg gacacagcag aaaccgctgt
      241 tccggttgcc cctacagaga ccaaacccga aggagggtca atcgaatgag ccggttactt
      301 ggatttgaag tcggcgtgtt gcgtcagtta cccggagaaa ccgtggccat tccctttggt
      361 cgcgatggtc gcctggggtg gtatttgaaa ctttccggga aacgggtctt ttccaatgat
      421 ttgcgccagt cggcaagctg gcaggcagtt gggaaggtgg aaaacaatgg gtatgttctg
      481 agcgcggttg agatccagca attacttgac gaagccgacg cttttgttca gcaaacccag
      541 ggaacacctg aaaatcagcg gctcctgggc tggtttgctc caacccaggc ggcctggctt
      601 gacgggtatc gaaaagcagc actcacgctg gatgaccggt cgttgcgtgg cctggctctc
      661 acccttggtc aacgaattgg cgaatactgg cgctcgtttg acagccagac ccaccacctg
      721 caattgccga ttgatgatgt gttgtctcac tttttggacg aactccatca agttgttgat
      781 aaccggcgcg aaaacctggc caccaacctt gatgcgcatc agtttgtgat tcaggtccat
      841 gcggatctgg tctatgtcca tctgccctca cccgaaccac tctcggctca gagtcagcaa
      901 cctgatggct ggaccgaagt ctggatcacc ggcaacccgg atatttggga aaccctcacc
      961 gccaatcagc gcaatcggtt cggcggccag ttgatgaccc gcaccaaata ccgcgaagtt
     1021 gtcaaaggct ttttaaaacg cctccggcac tttcccgcct gggtcatttc aatcaatcaa
     1081 cccggcccgc tttcaactga ggaaatggtg caattgatcg aagaacaccg cccggttgag
     1141 aaaatctatt ccaaagacgc gactgaattt ctcgatggat ttacccagca tttgattgtg
     1201 agtcgtctct gagtgcaaac aacagagaaa ggaaagaaat atttaccgtg gaagtcgaac
     1261 tctcaaaaac atttaccttt gaagccgccc accggctgcc caaggttccc cctggtcata
     1321 aatgcgcccg cctgcacggg catagttttc ggatcgaagt cgtggttcgg ggtccggttg
     1381 atccggaatc cggctggttg attgattatg cagagattaa agatgccttt gaaccactct
     1441 ttaaaatcct ggatcataac tacttgaatg aaattgaagg gcttgaaaac ccaaccagcg
     1501 aaaacctgtc ccgctggatt tgggaacgac tgagcactgg cgaaaataac ctggcaaggt
     1561 atctgtaccg cgtcaccgtt gctgaaacct gcacgtcggt ttgtcactac tttggtgcgt
     1621 agtccctggt tctgagttct tggttcttgg ttcttggttc ttggttcttg gctcttggtt
     1681 cttggttctt ggttcttggt tcttggttct tggttcttgg ttcttg
//
LOCUS       DAOQDO010001591         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74192_length_1726_cov_2.696589, whole genome shotgun sequence.
ACCESSION   DAOQDO010001591 DAOQDO010000000
VERSION     DAOQDO010001591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74192_length_1726_cov_2.696589"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKZ53_21005"
     CDS             complement(<1..913)
                     /locus_tag="PKZ53_21005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ42974.1"
                     /translation="MTIDWQARLETFLAIREALGLVSRPARILLQDFISHIESHHIPV
                     PVPAQVIVEWATSSRGASGHCARLSLARLFLEHVRAFVPETIVPPKGLLATRRRPHPY
                     LFAPEDICRLMESPITIRPHYTLRPYLVRTLVGLLASTGIRIGEALNLRVEDVSLDST
                     PPFLHIRNAKFHKSRYVPLHPTVAVQLSEYASIREAFGYAALSEMFFMSEQGTALTHT
                     SLQRTFSRLCQRLGISPINGHHPTLHSFRHTFAVQCLVSWSERGDDIHVRLPNLAVYL
                     GHLNPQETYWYLTATPQLLNPASLAFQR"
     gene            complement(910..>1726)
                     /locus_tag="PKZ53_21010"
     CDS             complement(910..>1726)
                     /locus_tag="PKZ53_21010"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNJ42975.1"
                     /translation="YLSLVRSFLSPIIQDPPLDWAWMNATKITDFICHHAQTHHGKGR
                     QAAAVAIRSWLRFMVGQGMLRAGLELCIPRIPTQRHAALPVTLTAEEINRILDTCDQS
                     TVSGRRNFTIFLMLARLGMRSGEIIRLRLDDIDWHSGQILVQAGKTRRQRRLPLSMEV
                     GEAIVRYLPDRPHSSHRTLFLSQQPPFVPLQHPSALSHLVARTLKRAGIRNVGGAHLF
                     RHTAATHMVCRGASFKDVADVLGHQSLQTTAIYAKLDLATLAEMAQPWPGGGL"
BASE COUNT          443 a          408 c          515 g          360 t
ORIGIN      
        1 aacgttgaaa agcaagcgaa gccggattca acagttgcgg ggttgccgtc aaataccagt
       61 aagtttcttg agggttgaga tgtccaaggt agacagcaag attgggcaat cggacgtgaa
      121 tgtcgtcacc ccgttcagac caactgacca gacactggac cgcgaaggta tgccgaaatg
      181 aatggagcgt cgggtgatga ccgttgatcg gggaaatgcc aagtcgttgg cagagacggg
      241 agaaagtacg ctggaggctg gtgtgggtca gcgctgtgcc ttgttccgac ataaaaaaca
      301 tttccgagag tgcagcatag ccgaaggctt ccctgatgga ggcatattca ctgagttgaa
      361 ccgccacggt tgggtggagg ggtacatatc tggatttgtg aaacttggcg ttccggatat
      421 gcaagaaagg cggggtggaa tccaatgaaa catcctccac tctcaaattg agggcttctc
      481 cgatccgaat cccggtgctg gccagcaaac ccaccaaagt tctgaccagg tatggccgca
      541 gagtgtagtg tggccgaatg gtgatgggag attccatcaa acggcagata tcttcgggtg
      601 caaacagata gggatgcggg cgtcgccggg tggcgagcag tcccttgggt gggacaatgg
      661 tttcagggac aaaggctcgc acgtgttcca gaaacaatcg agccaatgac aaccgggcac
      721 aatgacctga agcgccacgg gaactggttg cccattcgac gatgacctgc gcggggacgg
      781 ggacgggaat atggtgggac tcaatgtgag aaataaagtc ctgaagcaga attcgtgccg
      841 ggcgggaaac aaggccgaga gcttcacgaa tggccaggaa cgtttcaaga cgagcttgcc
      901 aatcaatggt cataatccac ctcctggcca cggttgagcc atttcagcca gggtggcaag
      961 atccagcttg gcgtaaatcg cggtggtttg aagtgactgg tgtcccaaca catcggccac
     1021 atctttgaag ctggcaccac gacaaaccat gtgagttgcc gccgtatgcc gaaacaaatg
     1081 tgcccctccc acattgcgga ttcccgcccg tttgagtgtt cgcgcgacaa gatgactcag
     1141 tgctgaagga tgttgcagcg ggacaaatgg tggctgttgt gacaaaaaca aggtgcgatg
     1201 tgaactgtgt gggcgatctg gaagatagcg aacgatagcc tcaccaactt ccattgaaag
     1261 tggaagccgc cgttgccgac gggtcttgcc agcttgaact agaatttgtc cggaatgcca
     1321 atcaatatcg tccaggcgca gtcggataat ctcgcccgaa cgcattccca accgtgccaa
     1381 cataaggaaa attgtgaaat ttctccgtcc actgacggta gactggtcac acgtgtcgag
     1441 aattcggttg atttcttcag ccgtcagagt taccggcaaa gcggcatgtc gttgggttgg
     1501 gattcgtgga atgcagagtt ccaaaccagc acgtaacatt ccctgcccga ccatgaatcg
     1561 cagccatgaa cgaatggcaa ccgccgccgc ctgacgtcct tttccgtggt gggtttgtgc
     1621 atggtgacag ataaaatccg ttatttttgt ggcattcatc caggcccaat caagaggggg
     1681 gtcctgaatg atgggggaga gaaaggagcg aactaaagac aggtat
//
LOCUS       DAOQDO010001592         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74341_length_1724_cov_2.982025, whole genome shotgun sequence.
ACCESSION   DAOQDO010001592 DAOQDO010000000
VERSION     DAOQDO010001592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74341_length_1724_cov_2.982025"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PKZ53_21015"
     CDS             complement(<1..1141)
                     /locus_tag="PKZ53_21015"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HNJ42976.1"
                     /translation="MHRLSRPFLFITALAVIGTAFWFSNSRANSGLFAFQSSPFGSTT
                     QTGSKLQSQPELLQLRKSDDGIWQDVEPSQIQARYPAGVVQRNVAPIAFRTINLDHLA
                     LKQILQRAPMESVETGLSSTAIISLPMPDGKFARFRFVESPIMESELAARYPDMKTYA
                     GQGIDDPTAVVRFDTVPQGFHAIILSVHGTTYIDPYLKNNTDLYASYYKRDFRKDAEP
                     FRCLFENPLHEDPESAHATRNFQPNVPTGANLRTYRAAVATTGEYTAAAGGGTLVGGL
                     GAVITSVNRVSAVYERELAIRLVLVNNEDKIIYTNSGTDPYSNSSASALLSENQSNLD
                     SVIGSANYDIGHVFSTGGGGLAGLGVVCRAGQKARGETGSGNPVGD"
     gene            complement(1541..>1724)
                     /locus_tag="PKZ53_21020"
     CDS             complement(1541..>1724)
                     /locus_tag="PKZ53_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42977.1"
                     /translation="MNRTQRYTTREHLPAGQVSLPPGDNLNAVDDFGPLPNRQTVGQP
                     ASVTESTTELLNNQSQ"
BASE COUNT          430 a          401 c          490 g          403 t
ORIGIN      
        1 gatcgccgac tggattgccc gaacccgttt caccacgggc tttttgccca gcacggcaca
       61 ccacgcccag tccggcgagt ccaccaccgc cggtgctaaa cacgtggcca atgtcataat
      121 tggcagaacc aatgaccgaa tccaggttag actggttttc gctcaacagg gctgaggcgc
      181 tgctgttgct gtaaggatcg gttccagagt tggtgtagat gatcttatct tcattgttga
      241 ccagcaccag gcggatagcc agttcacgtt cgtaaacggc actgacccgg ttgacgctgg
      301 tgatgacggc gcccaggccg ccaacgagtg tgccaccgcc agcagcggcg gtgtattcgc
      361 ccgtggtggc caccgcggca cggtaagtcc gtaagttggc gccggttggc acgttgggct
      421 ggaagttccg ggtcgcgtgg gctgattccg gatcttcgtg aagcggattt tcaaacagac
      481 accgaaatgg ctcagcatct ttgcggaaat cccgcttata gtatgacgca tagaggtcgg
      541 tattattttt caagtatgga tcaatgtacg ttgttccatg gactgaaaga atgatggcgt
      601 gaaagccttg tggcaccgta tcgaaccgga ccaccgccgt tgggtcgtca attccctgac
      661 cggcgtaggt cttcatatca ggatatctgg ccgccagctc agattccata attggcgatt
      721 caacaaaacg aaagcgggca aattttccat ccggcatcgg aagggaaatg atcgccgtgc
      781 tggaaagacc agtttcaact gactccatcg gggcacgttg caaaatttgc ttgagcgcca
      841 ggtgatcaag attgatggtt cggaaggcaa tgggcgcaac atttcgctga acgacacccg
      901 ccggatagcg agcctgaatt tgagacggct cgacatcttg ccaaattccg tcatccgatt
      961 tgcgaagttg gagcagttca ggttgtgatt gaagtttcga accagtttga gtggttgaac
     1021 caaaaggcga agactgaaag gcaaacagtc cactgtttgc acggctgttg gaaaaccaga
     1081 aagcggttcc aatcacagcc agggcagtga tgaaaagaaa cggtcgagac aatcggtgca
     1141 taaaccccca gtgattgttg ttgtaaactg acgtgacgat ctgcgttcaa acaggctggc
     1201 tcaggcggcc cctgacgggt caaaatatgc aggcacttga cagatatctg ttgactgagc
     1261 gagttgtttc aagcaagcga atctggcgag caaacggctt ttactgtggt gggatacagt
     1321 ctttaggaaa aggataccca caataaagga cgaaaagaga acaggtttct tccgttgatc
     1381 tctctgtctt taaggtaaag tactccataa agtagagata gagtctggaa gaaatgatgt
     1441 gtgacggaag tgaaacttgt gagaggagtc agcagtgaat gatctggcct ggtgctgagc
     1501 taaggcaggt tagacaacaa caaacgatgc ggcaggaggt ttactgactc tgattgttaa
     1561 gcaattcagt tgtgctttca gtcacacttg ctggttggcc gacagtttgt cggtttggca
     1621 atggcccaaa gtcatcaact gcattgaggt tgtcacctgg aggcaaggaa acttgccccg
     1681 ctggaaggtg ttcacgagtt gtatagcgct gtgtccggtt catc
//
LOCUS       DAOQDO010001593         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74651_length_1721_cov_2.140456, whole genome shotgun sequence.
ACCESSION   DAOQDO010001593 DAOQDO010000000
VERSION     DAOQDO010001593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74651_length_1721_cov_2.140456"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..342
                     /locus_tag="PKZ53_21025"
     CDS             139..342
                     /locus_tag="PKZ53_21025"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNJ42978.1"
                     /translation="MPKMTPLHWKRLEAIFLAAGFQFFRQEGSHRSYVKPGIARPVVI
                     PTYDEVPVFIIKNNLKNSRNLPR"
     gene            complement(452..1375)
                     /locus_tag="PKZ53_21030"
     CDS             complement(452..1375)
                     /locus_tag="PKZ53_21030"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HNJ42979.1"
                     /translation="MTHARRLKTAVLYVVWLLMIVICTAPNVQADPSVRFKLVQKHLI
                     VALVSVNGQGPFEFLIDTGTHLTLVDPLLVEELQLKSGTQVSLMTISGKQSVPRSVLK
                     SVQVGTALLEHLEALTVDLTELRRVRPQIRGMLGQNFLSRFNYLIDYHNQRIVFEEHQ
                     EFEHQATGTRLPFVLDSGKILISAQTSNHPAGELQLILDSGTPGLMLFEPTLQTRKVP
                     VERNGGFIMMAAATTGVRPTQTGRLARVRVGDEFFENLPVTFVRGDSIAKTFAANGLL
                     PTNLFQTLYVNNQKKFVVMNAETEAGMIVRK"
     gene            complement(1496..>1721)
                     /locus_tag="PKZ53_21035"
     CDS             complement(1496..>1721)
                     /locus_tag="PKZ53_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42980.1"
                     /translation="LIKTGSLDEFGDRGSMLGNVETMLSFAKESNSMKNQTSLFAARL
                     QTLWVRAKPPVRLCKPGISRPVPERHQIDT"
BASE COUNT          432 a          432 c          418 g          439 t
ORIGIN      
        1 tcttgaaaca gcaactgagt gtggaactct cgaggacgtg cttgaagatg caggttacac
       61 acttcaggat gggcaatgga tcagtccggt gtgggtatcc attgagcgac attcattact
      121 ggttggagtg tgagtgcgat gcctaaaatg acaccgcttc attggaaacg gctggaagct
      181 atttttctgg cggctggatt ccaatttttt cgtcaggaag gcagtcaccg ttcctatgtt
      241 aagcctggga ttgcacgtcc agtcgtcatt cctacctatg acgaagttcc agtgttcatc
      301 atcaaaaaca atctgaaaaa cagtcggaat ctcccgagat gaattttttc agcttcttga
      361 tcaggtataa aagttgggga ataaattctc tgaattcaac tttttcctga ccctgagccc
      421 gaagtcttca gcccaaagtc ttcagcccca cttatttcct cacgatcatt cctgcctcag
      481 tttcagcatt catcacgaca aatttcttct gattattgac atagagtgtt tgaaaaaggt
      541 ttgtggggag taacccattg gccgcaaagg ttttggcaat cgagtcaccc cgcacaaacg
      601 tcactgggag attttcaaag aactcatcac ccaccctcac ccgcgccagc ctgccagttt
      661 gtgtcggcct gacaccagtc gtggctgccg ccatcatgat aaaccctccg ttgcgctcca
      721 ctggcacctt gcgggtctgc aaggttggct caaacagcat cagtccgggc gttcctgaat
      781 ccaaaatcaa ctgcaactca cccgctggat gatttgaagt ctgggcggaa atcaggattt
      841 ttccggagtc gagcacaaag ggcagccggg ttccggtggc ctggtgctca aattcctggt
      901 gctcttcaaa cacgatccgc tgattgtgat agtcaatcag gtagttgaac cgactcagga
      961 aattttgccc gagcatcccg cggatctgcg gacgaacccg gcgcaattcc gtcaaatcaa
     1021 ccgtgagtgc ttccagatgc tctagcaaag ccgtgcccac ctgtaccgat ttcaaaactg
     1081 agcgtggtac actttgcttc ccggaaatcg tcatcagcga aacctgagtg ccggatttca
     1141 actgcaattc ctcaaccagc aacgggtcaa ccagtgtcaa atgagtgccc gtgtcaatga
     1201 ggaattcaaa cggtccctga ccattgaccg aaaccagtgc cacgatcagg tgcttttgaa
     1261 ccagtttaaa gcgaacggat gggtcggcct gcacgtttgg ggcagtgcat atcacaatca
     1321 tcaagagcca gaccacatac aaaacagcag tcttgagccg gcgagcgtgg gtcatagagt
     1381 cctccgaaat ggcgaagttg aggtgactct aaacccaggc caaacggcgc gtcttgatgc
     1441 cgttatgacg tttcaacgtg tttcggtgat gaagttatga tgaaagccaa actccttatg
     1501 tatcaatttg atgacgttca ggtacaggtc gagaaattcc gggtttacaa agccggacag
     1561 gtggtttcgc tcgaacccaa agcgtttgaa gtcttgcggc aaaaagggaa gtctggtttt
     1621 tcatgctgtt tgattctttt gcgaaagaaa gcatggtctc tacgtttcct aacatagagc
     1681 ctctgtctcc gaactcgtcg agtgatccag ttttgatcag t
//
LOCUS       DAOQDO010001594         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74706_length_1720_cov_2.923724, whole genome shotgun sequence.
ACCESSION   DAOQDO010001594 DAOQDO010000000
VERSION     DAOQDO010001594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74706_length_1720_cov_2.923724"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKZ53_21040"
     CDS             <1..1091
                     /locus_tag="PKZ53_21040"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNJ42981.1"
                     /translation="GIHILGEVPDRALDAIAGTGERLSARIVAAAIRNIGLRAKAIDA
                     TELVVTDDNFGDATPDSIETNLKVQTRLLPLLRDGVIPVVTGFIGATPQGVMTTLGRG
                     GSDYSGGILGAALNADEVIIWTDVNGFMTADPNLVPDAQTIPQISYSEAAELSYYGAK
                     VLHPKTLLPLAPAKIPLYIKNSFEPDHPGTHITSQTGGWNADIKAITAIKNLSLVTIS
                     GSGMMGVPGIAAKTFSAVASQSINVLMISQSSSENNLCFIVDSAEAERTRTALRKALD
                     IEFHHRHIENITVTDGIAIVAAVGEQMHGRPGIAARVFSAVADAKVNVIAIAQGSSEI
                     NISFVVDAKDVPAAVVAMHKRFELGNGK"
     gene            1153..>1720
                     /locus_tag="PKZ53_21045"
     CDS             1153..>1720
                     /locus_tag="PKZ53_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42982.1"
                     /translation="MTDLLLTQKLAALETEPWPLVDLRQVIDLSTSPAHRRVSFPAPE
                     YTPPFQILDRQILGKLIGVELDQTEGIFSEVKDVSSNRAGVCLVTSSVFGRWNAHAGS
                     HADQPIHWLKDPPLAQFDDRQYRGPATVLNLTSFLAGQSPPAITLDILTQAMTQARID
                     PLTVRRLVVRTYHHTPVEWDDTFAYLTPE"
BASE COUNT          380 a          498 c          454 g          388 t
ORIGIN      
        1 acggcattca tattctgggg gaagttccag atcgggcgct ggatgcgatt gccggaaccg
       61 gcgaacggct ctcagcccga atcgtcgccg ctgccattcg caacatcggg ttgcgggcca
      121 aagctattga tgccactgaa ctggtggtca ctgacgataa tttcggcgat gccacccccg
      181 atagcattga aaccaatttg aaggttcaga cccgattgct cccgctcctc cgggatgggg
      241 tcattccagt ggtgactgga tttattgggg caacaccgca gggcgtgatg accacactcg
      301 gacgtggcgg ctcagactac tcagggggaa tccttggcgc ggccctcaat gccgatgaag
      361 tcattatctg gaccgacgtc aacgggttta tgacggcgga tcccaacctg gttcctgatg
      421 cccagaccat tccccaaatc agttacagcg aagcagccga gctatcttac tatggcgcga
      481 aggtattgca tccaaagacg ttgcttccac ttgccccagc caagatcccg ctctatatca
      541 aaaacagctt tgaacccgat catcccggca cccatattac ctcccaaacc ggcggctgga
      601 atgccgatat caaggccatc acggccatca aaaatctgag cctggtgacc atcagcggga
      661 gcgggatgat gggtgttccg gggattgcgg ccaaaacttt ttccgccgtg gcgagtcaga
      721 gcatcaacgt gttgatgatt tcccagtcat cttcggaaaa taacctctgc tttattgttg
      781 actctgctga agctgagcgg acccgcacgg ccctgcgcaa agccctcgac attgagtttc
      841 accaccgcca catcgagaac atcacggtga ccgatggcat tgcgattgtc gctgcggttg
      901 gtgagcaaat gcatggtcgc ccaggcattg ccgccagggt cttttccgcc gtcgccgatg
      961 ccaaagtcaa cgtcatcgcc attgcccagg ggtcatcaga aatcaatatt tcatttgtgg
     1021 ttgatgccaa agatgttccg gcggcggtgg tcgccatgca caaacgcttt gaattaggga
     1081 atgggaaata acctggagtg cggcggcttg caaagcggcg tcaagccgcc gcactccgaa
     1141 aagaatcacc ctatgactga cctcctcctg actcaaaagc tggctgccct tgaaaccgaa
     1201 ccctggccgc tggttgatct tcgccaggtg attgacctga gcacatcgcc tgcgcaccgg
     1261 cgggtgagct tccctgcccc agagtatact ccgccgtttc aaatccttga tcggcagata
     1321 cttggaaaac tcattggtgt cgagctggat caaacggaag gtattttttc ggaagtgaaa
     1381 gacgtttcct ccaatcgagc cggggtttgt ctggtgacat ccagtgtttt tggccggtgg
     1441 aatgcccacg ccggctcgca cgctgaccag ccaatccact ggttaaagga tcctccgctg
     1501 gctcagtttg atgacaggca atatcgtggg ccagctacgg ttttgaatct cacgtcattt
     1561 ctggctggtc aatcaccacc tgcgattacc ctcgacatcc tgactcaggc catgacccag
     1621 gcccggattg atccactcac tgttcgccgg ctggttgtgc gcacctatca ccacacacca
     1681 gttgagtggg atgacacttt tgcctacctg acacctgaag
//
LOCUS       DAOQDO010001595         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74848_length_1719_cov_1.814904, whole genome shotgun sequence.
ACCESSION   DAOQDO010001595 DAOQDO010000000
VERSION     DAOQDO010001595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74848_length_1719_cov_1.814904"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1719
                     /locus_tag="PKZ53_21050"
     CDS             <1..>1719
                     /locus_tag="PKZ53_21050"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ42983.1"
                     /translation="TVVEETIEVVLTVLDPAGKTILTHNIPDRESCTFRFLWLAETAG
                     TYQLQVTPKINFYPRGTYRLTGVSMRKATSQDQKWFRAIQLMNEGDRWRPQRDLNLVK
                     TAIPALKEAKRLFYELGDTFFEASVTNLLAIAVQYLDGLPAAIPIYEETLALRIQAGD
                     VTGARQTALNMAAVYFRLGDKEKCFACCLKARKDFAASTDRYLQLLVYTYEMAYRTGF
                     ENYTDLDELAAKGIQVARELGFTANIVSLTVNYGIHLRNRGELSHSMQVFHEGLKLAQ
                     EFNVPEFEGTANEQLAEIYFALGDLDQSLHFRLKAVDCFTQAKSAVNVLNNQVGMAII
                     ALRKGEYQKALDQFHQLLASARQLKSKINEGNILYNLSLLAVETGDYEKAMDYNDQSL
                     LAFDGNPNPIYQIQAINHKINLMVGYYKDRKALDLSEKVIAQVQTFPDKRFECYLRTN
                     YATALSYFGDYEKALAQYETILNLWQRVGDRYGEGHTLLRMGSVAYSLKQPDRARENF
                     LKALPICQATFSHDNVASCYLGLAVLERDAEHYSAALENSLKAIEINESLRANFQDQQ
                     ARSNLL"
BASE COUNT          446 a          420 c          436 g          417 t
ORIGIN      
        1 gacagtggtt gaagaaacta tcgaagtcgt cctgacggtt ctggatccgg ctggaaaaac
       61 gatcctcacc cacaatattc cggatcggga aagctgtacg tttcgatttt tatggctggc
      121 cgaaaccgcc ggaacctatc agttgcaggt cacaccaaag atcaattttt atccgcgcgg
      181 cacctatcgc ctgaccggtg tcagtatgcg aaaggcaact tcccaggatc agaaatggtt
      241 tcgagccatt caactgatga atgaaggcga ccggtggcga ccccaacgcg atctcaacct
      301 ggtcaaaacc gcgattcccg ccttgaaaga agccaaaagg ttgttttatg agctgggcga
      361 tacatttttt gaagcttcgg taaccaacct gctggcgatt gccgttcagt atctggatgg
      421 actgccggcg gcgattccga tctatgagga aaccctggcg ctgagaatcc aggccggaga
      481 tgtgaccggc gcccgccaga ccgcattaaa catggcggcg gtctatttcc ggctgggtga
      541 caaagaaaaa tgctttgcct gttgcctcaa agcgcgaaaa gactttgcgg cctcgactga
      601 ccggtatctt cagcttctgg tgtataccta tgagatggcg tatcggaccg ggtttgaaaa
      661 ttacactgac ctggatgaac tggccgccaa aggcattcag gtcgcccgcg aacttgggtt
      721 taccgcaaat attgtctcat tgaccgtcaa ttatgggatt cacctccgca atcgaggcga
      781 actctcgcac agtatgcagg tatttcacga gggcctgaag ctggctcaag aattcaacgt
      841 gcctgaattt gaaggaacgg caaacgagca actcgcagaa atatattttg cccttgggga
      901 tctggatcaa agtcttcatt ttcgtctcaa agcggttgac tgttttaccc aggctaaatc
      961 ggcggtcaac gtcctcaata atcaggtcgg catggccatc attgccctgc gcaaaggtga
     1021 atatcaaaaa gcattggacc agtttcatca gttgttagcg tcggctcgcc agctcaagtc
     1081 aaaaataaat gaaggcaaca tcctctataa tctgagtctg ctggcggttg aaactggtga
     1141 ttatgaaaag gcaatggatt ataatgacca gtctctcctg gcttttgacg ggaacccaaa
     1201 tccaatctat caaatccagg caattaacca taaaatcaat ttgatggttg gatattacaa
     1261 agaccgaaaa gcgcttgatc tttccgaaaa agtcattgcc caggttcaaa cgtttccaga
     1321 taaacggttc gagtgttatt tacggacaaa ttacgccact gccctgagct atttcggtga
     1381 ctatgaaaag gcgctggctc agtatgaaac gattctgaac ctctggcagc gtgtcgggga
     1441 tcggtatggt gaaggtcaca ccttgctgcg aatgggcagt gttgcctact ctctgaaaca
     1501 accggatcgg gcgcgggaaa attttctcaa ggccttgcca atctgccagg ctaccttcag
     1561 tcacgacaat gtggcgtctt gttacctggg gctggctgtt ttggagcgcg acgctgaaca
     1621 ttactccgct gcgcttgaaa acagcctgaa ggcgattgag atcaacgaat cactccgcgc
     1681 caattttcag gaccagcagg cgcgcagtaa tttgttgag
//
LOCUS       DAOQDO010001596         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74875_length_1718_cov_3.229104, whole genome shotgun sequence.
ACCESSION   DAOQDO010001596 DAOQDO010000000
VERSION     DAOQDO010001596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74875_length_1718_cov_3.229104"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKZ53_21055"
     CDS             <1..399
                     /locus_tag="PKZ53_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNJ42984.1"
                     /translation="NRLWYFGFFCLVIVAGLASRRFGPILPKFIAAYAGDTLWALMVF
                     LGFGMLWPRRSTLWLAAMAAGFSYLIEFSQIYHAPWIDAIRQSRLGGLVLGRGFLWSD
                     LVCYTIGILIGFGLETIWSKMRANHPTSPQ"
     gene            471..1397
                     /locus_tag="PKZ53_21060"
     CDS             471..1397
                     /locus_tag="PKZ53_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ42985.1"
                     /translation="MKLTVSNVLNQRFSIIGMLILVFLVPLAGLVPTAAQTPASGGSM
                     VKKHAQFSSKLIDPRDVDVWLPPGYETDTHKRYPVLYMHDGQNIFDPKTSYGGVDWGV
                     DETMDRLIREKKIRPAIVVGIWNSPKRYAEYMPQKAAKDPDTAHLKNIPINATGPIVS
                     DKYLKFLVTELKPFIDAHYRTLTDRKNTSIMGSSMGGLISAYAISEYPNVFGGAGCVS
                     THWPAGEGSMIEYLKNHLPDPRTHKIYFDYGTATLDSTYEPFQLKMDEVMKQHRYKAG
                     KNWITRKFEGAEHSEKSWRERVDIPLVFLIGK"
     gene            complement(1502..>1718)
                     /locus_tag="PKZ53_21065"
     CDS             complement(1502..>1718)
                     /locus_tag="PKZ53_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ42986.1"
                     /translation="ARSWASLLEEYTTVRAATVSLIKGLPSDAWLRRGTANKNPVSVR
                     ALVCLIAGHELHHMTIVKERYLTQLSQ"
BASE COUNT          423 a          432 c          418 g          445 t
ORIGIN      
        1 aaccgtctct ggtactttgg gttcttctgc ctcgtcattg tggctggttt ggcctctcgc
       61 cggtttggac cgatccttcc aaaatttatc gccgcttatg ccggagacac gctctgggca
      121 ctgatggttt ttttgggctt tggaatgctc tggcccagac gatcaacttt gtggctggct
      181 gcgatggcgg ctggattttc ctatctgatt gaattcagcc agatttatca cgcaccgtgg
      241 attgatgcaa ttcggcaaag tcgcctgggt gggctggtgc ttggacgcgg ctttttatgg
      301 agcgatctgg tctgctacac catcggcatc ctgattggtt ttggattaga gaccatttgg
      361 agtaagatgc gggccaatca tccgacttcc ccccaataac aactcacgtt cttgaatttt
      421 cccaatcata tcagtggggt atccagtttc ttcaggaaag gaaccatgcg atgaaattga
      481 ctgtttccaa tgtactcaac cagcgtttct ccatcattgg aatgcttatc cttgtgtttt
      541 tggtcccatt ggcggggttg gttccaaccg cagctcaaac cccagccagc ggaggaagta
      601 tggtcaaaaa acacgcccaa ttcagctcca aactgattga tccccgtgac gtagacgtgt
      661 ggctgccgcc gggatatgaa accgatacac ataaacgcta tccggtgctc tacatgcacg
      721 acgggcaaaa tatctttgat ccaaagacgt cgtacggcgg tgttgattgg ggtgtggacg
      781 aaaccatgga ccggttaatt cgagagaaga aaattcgccc ggcgattgtg gtcggcatct
      841 ggaatagccc caaacgctat gccgagtata tgccccaaaa ggcggccaaa gacccggata
      901 ccgcccacct gaaaaatatt ccaatcaatg ccaccgggcc gattgtgtcg gacaagtatc
      961 tcaaatttct ggtcaccgaa ctcaagccct ttattgatgc tcattaccgc acactgactg
     1021 accgtaaaaa cacttccatt atgggttcga gtatgggcgg gttgatttcg gcctacgcca
     1081 tcagcgaata ccccaacgtc tttggcggtg ccggttgcgt ttcaacccac tggccagccg
     1141 gtgaaggatc aatgattgag tatttgaaaa accacctccc tgacccacgc acacacaaga
     1201 tttatttcga ctatggcacc gccacgctgg attcaaccta tgaaccattc caattaaaaa
     1261 tggatgaagt catgaaacaa catagataca aagctgggaa aaactggatt accagaaaat
     1321 ttgaaggcgc cgaacattca gaaaaatcct ggcgtgagcg ggttgatatt ccgctggtgt
     1381 ttttgattgg aaaataacac cagggacttt ggtgctgagt aatcattcta tgtttccaat
     1441 cattgatcta ttgttcagga gcgaactcaa gtgaagttca ctcctgactt tcaacagatc
     1501 attactggga aagctgtgtg aggtatcgtt ctttcacaat cgtcatgtgg tgcaattcgt
     1561 gcccggcgat caaacaaacc agcgcccgaa cgctcactgg attcttgttt gccgttcccc
     1621 gccggagcca ggcatcggag ggtaaccctt tgatcagact gaccgttgcc gcacgaaccg
     1681 tggtgtattc ttcaagcaaa cttgcccatg aacgggca
//
LOCUS       DAOQDO010001597         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_74900_length_1718_cov_2.457607, whole genome shotgun sequence.
ACCESSION   DAOQDO010001597 DAOQDO010000000
VERSION     DAOQDO010001597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74900_length_1718_cov_2.457607"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PKZ53_21070"
     CDS             complement(<1..1121)
                     /locus_tag="PKZ53_21070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ42987.1"
                     /translation="MSSVSLTASRKAVSICFFLLGASYANWLSRIPTVQQNLNLSDGM
                     LGLALLGPGIGALIAMPTAGWLIARIGSRPVTVLSSIATCISIILPGWATNGPTLFVA
                     LLILGITSGAMDVAMNTQAVEVEKAYGRHIMASFHALFSIGGLTGAAMGGGVAFFHIQ
                     PAVHLLCAGLVFAFVAGAASRYLLSTAPTHESKNQPVFVRPSKALWALGLIAFCAFLS
                     EGAMADWGAVYLHTALKTSDALAATGYIVFSLFMTTGRITGDAITHRFGSVSTVRTGS
                     LLAGAGLAFSLFSSTYWLALLGFATMGAGYACIVPAVFSAAGRSKVMQPGIALAAVTT
                     LGYLGLLLGPPVIGFLSEAFTLRGGLSLIVLLSVIIAVLS"
     gene            1451..>1718
                     /locus_tag="PKZ53_21075"
     CDS             1451..>1718
                     /locus_tag="PKZ53_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ42988.1"
                     /translation="MEYSSNKAVRIVAISDTHSMHRALVLPEGDILIHAGDLTGRGQL
                     EEIEEFNCWLGMLPFQYKIIVAGNHDWAFQLTPDIARRMLTNGIY"
BASE COUNT          480 a          427 c          433 g          378 t
ORIGIN      
        1 gagaggacag caattatgac gctgagcaac acaatcaggc tgagcccgcc tcgcaaggta
       61 aacgcctccg ataaaaagcc aatcactggt ggtccaagca ataacccgag atagccaagt
      121 gtcgtcacgg cagcgagggc aatccctggt tgcatgactt ttgaccgtcc agcagcgcta
      181 aacaccgccg gaacgatgca ggcatacccg gcgcccatcg ttgcgaaccc caaaagtgct
      241 aaccagtatg tacttgaaaa taaagagaat gccagtccag ctccggccag caggcttcca
      301 gttcgaacgg ttgaaaccga gccaaaccga tgtgtaatgg catcaccggt gattcggcca
      361 gttgtcataa agagtgaaaa aacgatatac ccggtggcag ccagggcatc agacgttttt
      421 aaggcagtgt gcaggtacac cgcgccccaa tcggccatcg cgccttcact caggaaggca
      481 caaaaggcaa tcagtccaag ggcccacagc gcttttgagg gcctgacaaa gactggctgg
      541 ttttttgatt cgtgggttgg cgccgttgac agtaaatacc gtgatgccgc acctgcgacg
      601 aaggcaaaaa cgagcccggc acacaacaaa tgaactgctg gctggatatg gaaaaatgcg
      661 actccaccac ccatcgcggc gccggttaaa cctccgatac taaacaaggc gtgaaacgac
      721 gccatgatgt ggcggccata ggctttttca acttcgacgg cctgggtatt catcgccaca
      781 tccatggccc ccgaagtgat acccaggatc aaaagcgcta caaacaaggt tgggccattc
      841 gtcgcccagc ccggcaaaat aattgatata caggtggcaa ttgaagacaa cactgtcact
      901 ggccggcttc caattctggc aatcaaccat ccggcggtgg gcatggcaat cagcgcccca
      961 atccctggtc cgagcagcgc caaccccagc atgccgtcac tcaggttgag gttttgttga
     1021 acggttggaa tgcgcgataa ccagttggca taactggcgc ccaacaggaa aaaacaaatg
     1081 gaaacggctt ttcgggacgc cgtgagtgaa actgaagaca tatcaatgtt ccttgatctc
     1141 gattcaaata tgcgagcgcc agttcaacga aaagaattgc acgaaagttc gtgtttctgc
     1201 atattactgc aagtttctgc ttgcttaaag ccggatttac cggatttttg attgattcgg
     1261 gcaaaaaacc gctcaaagat gattgttttt caggtgagtg tactgtggaa tcctgatgac
     1321 agtgctttat ttccagttct gaaagaatgg atggccgatt gtaaaatgtg ccgattcctc
     1381 aaagatccat ctgcctgaca atcgaaagcc tgtgttaccc ttttggaaat aaaaagaaaa
     1441 gaaaagaact atggaatatt catcaaacaa agcggtgcgg attgtggcga taagtgatac
     1501 ccactctatg catcgggcgc tggttttgcc cgaaggtgat attttaatcc atgccggaga
     1561 tttgactgga cgtggtcaac ttgaagaaat agaagagttt aactgctggt tgggaatgct
     1621 tccgtttcag tataaaatca ttgttgctgg caaccacgat tgggcgtttc agcttacgcc
     1681 ggacattgcc cgacgaatgc tcaccaatgg tatttact
//
LOCUS       DAOQDO010001598         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75169_length_1715_cov_2.146988, whole genome shotgun sequence.
ACCESSION   DAOQDO010001598 DAOQDO010000000
VERSION     DAOQDO010001598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75169_length_1715_cov_2.146988"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1715
                     /locus_tag="PKZ53_21080"
     CDS             <1..>1715
                     /locus_tag="PKZ53_21080"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNJ42989.1"
                     /translation="IFQIVSDYAERNRIYPKTLADVERILSSHGMKFSQITDPWGMPF
                     QSEFVSVLGLDKWRIKSSGPDKKPGTDDDFLAGELGWSPFTKTGDVIDRVLRMYHQKT
                     GGYIRDVETLRRELALEGINLSDLRDRWGRPLVCEFTIEEHWFNLYLKSAGEDGKLNP
                     RSETHLPLDDFVVWKSTISYFDDIRKVIDVVMRTSASVIGQAENKEVVFRQLLKTEGL
                     DFDGLRDPWGNAYQVKFEKRVPDDNESSSASASAPSKLEFWTIKISSLGPDGPKETWD
                     DFDLGLFSTLVSREKSAAGSTVLSTGSGGAVIATVLDATDAVISGAKVELRSEWRGSI
                     YTALTDGAGRVRFEDLAPGLYSATASAIGFKSTRWVNISVQKSEIAFLKIYLEPGAAE
                     NAVAVTAGPRLKQTSPAETVVKAKDSHPSRPLVQLSTPRLRKYFPETMLWEPLLETDQ
                     TGQVRIHFNLADNVTTWKINALASTMNGELVTGEAEITAFQPFFIEHDPPQVLTVGDE
                     LSLPVLLRNFLPDSQTMTVSVKPESWFELRGPSQKQVEVAAGGTAKPVFPLRATQMVK
                     HGPLQ"
BASE COUNT          439 a          412 c          459 g          405 t
ORIGIN      
        1 catttttcag attgtttccg actatgctga aagaaatcgc atttatccca agactttagc
       61 cgatgtcgaa cgcattctgt cgtcccacgg catgaaattc agccaaatta ccgatccctg
      121 gggaatgccc tttcaatcag aatttgtctc ggttttgggc cttgataaat ggcgaatcaa
      181 aagcagcggg ccggataaaa aaccagggac agatgatgat tttctcgccg gggaacttgg
      241 ctggtcgcca tttacaaaaa ctggggacgt gattgaccgg gtgcttcgga tgtaccatca
      301 gaaaaccgga gggtacattc gtgatgttga aacactccgc cgtgaacttg ccctggaagg
      361 aatcaatctg tctgatctcc gtgaccgatg gggccgtcca ctggtgtgcg aattcacgat
      421 tgaagaacac tggtttaacc tctacctgaa aagtgcaggt gaagatggca agttgaatcc
      481 gcgaagcgaa acccatctcc cgctggatga ttttgtcgtg tggaaatcca ccatcagcta
      541 ttttgatgat atccgcaaag tcattgatgt ggtgatgcgc acgtcggcaa gcgtgattgg
      601 acaggctgaa aacaaagagg ttgtgttcag gcaactgctg aaaactgaag gacttgattt
      661 tgatgggctt cgtgatccgt ggggaaatgc ctatcaggtg aaatttgaaa aacgagttcc
      721 agatgataat gaatcatctt ctgcctcagc ctctgcaccc tcgaagcttg aattttggac
      781 catcaaaatt tccagtttgg gtccagacgg accaaaagaa acctgggatg atttcgacct
      841 gggtctgttt tcgaccctgg tcagtcgaga gaaatcagca gccgggtcaa cagtgctttc
      901 aaccggaagc ggtggggctg tgatagcaac tgttttagat gccacggatg cagtcatatc
      961 aggggcaaaa gttgagttgc gaagtgaatg gcgtggcagc atctacacgg cgctcacaga
     1021 tggagccggc agggtccggt ttgaggatct ggcacctggc ctgtattcag cgactgcatc
     1081 tgcaattggg tttaaaagca caaggtgggt aaatatttca gtccaaaaat ctgagattgc
     1141 tttccttaaa atatacctgg aaccaggagc cgcggagaat gcggtagccg tcaccgctgg
     1201 gccacgtctg aagcaaactt caccagccga gacggttgtc aaagccaaag actcgcaccc
     1261 atcacgaccg ctggtgcaac tttcgacacc ccggctgcgc aaatattttc cggaaaccat
     1321 gctctgggaa ccgctgcttg agaccgacca aaccgggcag gtgcggattc acttcaatct
     1381 ggctgacaat gtaaccacct ggaaaatcaa cgcgctggct tcgacgatga atggcgaact
     1441 ggtgaccgga gaagccgaaa tcacggcctt ccagccgttt ttcatcgagc acgatccgcc
     1501 acaggttttg acggttggcg atgaactttc gctcccggtg ctgttgcgca acttcttgcc
     1561 ggattcacag actatgacgg tttcagtcaa acctgaaagc tggtttgaac tgcgaggtcc
     1621 ttcgcaaaaa caggtggaag tagctgcggg tggcactgca aagccggttt ttccgcttcg
     1681 agccactcaa atggtaaagc acggcccgtt gcagg
//
LOCUS       DAOQDO010001599         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75218_length_1714_cov_3.764919, whole genome shotgun sequence.
ACCESSION   DAOQDO010001599 DAOQDO010000000
VERSION     DAOQDO010001599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75218_length_1714_cov_3.764919"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKZ53_21085"
     CDS             <1..395
                     /locus_tag="PKZ53_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42990.1"
                     /translation="VAVTKTVTQIKGLPFLINAGYKATNGSLFGIAGNAPAWKGRAFG
                     AVAFVVPGPAKSTLILGSEFAQQPRDIEGLPGAVMPTSLTYFVRILPNKEVPMAIDFG
                     VAQAAGKILPGVDLKARTQFAMGLSYRF"
     gene            872..1639
                     /locus_tag="PKZ53_21090"
     CDS             872..1639
                     /locus_tag="PKZ53_21090"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNJ42991.1"
                     /translation="MVELYAAFLFGLLGSLHCIGMCGPIVLAYSLPLSAKEKARRVFW
                     HHLFYHFGRVTTYGFLGAVAGGIGSQMYVVGDWVGAGRIVLLIFGLVLLAVAIILAGG
                     SRLFPWLSFDFVTQSAMYKQTVGKLMVSSAPASKYALGLLLGFLPCHLIYAMLIQAAA
                     FGSIRTGFLMMVCFGAGMVPALLGIGFASQIISQPVRRWGERLVTASLVILAVTFLLR
                     SVGYNAMHFGHPPMAAPGPVVAGAPPSPEDHGGGGCH"
BASE COUNT          344 a          442 c          458 g          470 t
ORIGIN      
        1 atgtggcggt taccaaaacc gtgacccaaa ttaaagggtt accattcctg atcaatgctg
       61 gatataaagc caccaacggc agtctctttg gaatcgccgg aaatgccccc gcctggaaag
      121 gccgcgcatt tggcgccgtg gcgtttgtcg tccctggacc agccaagagc acattgattc
      181 tcggttcaga atttgcccag caacctcgtg acatcgaggg gctcccgggt gccgtcatgc
      241 caacctcctt gacctacttt gtgcgcattc tgccgaacaa agaagtccca atggccattg
      301 atttcggcgt ggcacaggct gccggcaaga tcctgcccgg tgttgacctc aaggccagaa
      361 cgcagtttgc gatgggtctt tcatatcggt tttaagtgta tgaaggagca aacagagagc
      421 gagtagcgaa tagatcgtga gtggtgaata gcgagtcgcg agatagtcaa tccagcacag
      481 agcttgtact caatattcaa ccattctgaa caccagagtt accgaataca tattcctaaa
      541 ctttttctga tggttcgcta cccgctactc gctatctgtt cacggctcac gctttatgga
      601 ttgactatct cgcgactcgc tactcactat ctgcttgcta ttttattcag ttttttcttg
      661 ttactcccta cgtttctctc cgagcccagc cagtggacaa ccacgaactg ggcctttttt
      721 tcttaaactc aaagtgggga aaagacactg ggctcaaaac ttggggctga agactcgcaa
      781 gctcggggct gaattacccg tcttcttcag cccgttttct tcagcccaaa gttcttcagc
      841 ccaaagttct tcagtcagag gttttcacaa tatggttgaa ttatacgctg cttttctgtt
      901 tggtttgctt gggagtcttc actgtatcgg gatgtgtggg ccgattgtgc tggcctacag
      961 tttgccactc agtgccaaag aaaaggcgcg tcgggtgttc tggcatcacc tgttctatca
     1021 ttttggtcgt gttaccacct atggtttttt aggtgcggtc gcgggcggaa tcggcagcca
     1081 gatgtatgtg gttggtgact gggttggcgc cggacggatt gtccttctga tctttgggct
     1141 ggttttgctg gctgtggcga ttattttggc cggaggaagc cgcctgttcc cgtggctctc
     1201 atttgacttt gtgacgcaat cagccatgta caaacagacg gttggaaaac tgatggtttc
     1261 atcagcacca gcatcgaaat atgcgcttgg cctgctgctt ggcttccttc cctgtcatct
     1321 gatttacgcc atgctgattc aggccgccgc ctttgggagc atccgaaccg ggtttctgat
     1381 gatggtctgt ttcggcgcgg ggatggtgcc agcattactg ggaatcgggt ttgcctcgca
     1441 aatcatcagc cagccagttc gccgctgggg agagcgtctc gtcacagcca gtcttgtcat
     1501 tctggccgtt acatttttgc tcaggtcagt tggatataac gccatgcatt ttgggcatcc
     1561 accgatggcc gctcctggcc ccgttgttgc cggcgcccca ccctccccag aggatcatgg
     1621 cggaggcgga tgccattaag aacctgggct gaagacttcg ggcttagggc tgaagacttc
     1681 gggcttaggg ctgaagactt cgggcttagg gctg
//
LOCUS       DAOQDO010001600         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75258_length_1714_cov_2.452682, whole genome shotgun sequence.
ACCESSION   DAOQDO010001600 DAOQDO010000000
VERSION     DAOQDO010001600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75258_length_1714_cov_2.452682"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKZ53_21095"
     CDS             complement(<1..245)
                     /locus_tag="PKZ53_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42992.1"
                     /translation="MHSRPHLSQLGLMLTAILAFGLAYQEWPLSVGCGLGVLSAYGNQ
                     FEFGWPTRNQMKRTGVAVLAILIISGGYYLAFFSSITP"
     gene            complement(428..>1714)
                     /locus_tag="PKZ53_21100"
     CDS             complement(428..>1714)
                     /locus_tag="PKZ53_21100"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ42993.1"
                     /translation="PSAAEIAAGTATLSLTTNDPPGACAPVFDTVVITIEPRVTVDAG
                     NNQTLCSDNPMVTLNGSIGGSATSATWSGGSGSFSPNASALNAVYMATLAEMQSGSLT
                     LTLTTNDPAGVCGPASDSVTIFFINCGSDTVLWVADTNNNRIQRFDGQNWSVVGVGTV
                     GSGDGQFRLPEAVVASSDSQRIYVADTGNNRIQWSTDGGNTWANFATNGTGLNQVRSP
                     KGLAYDALGNLYVSDNGNGRVVRFQAGLPGNSVVIASNGTASGQIMSPHGMVIDGTFR
                     LFVTDEAQSRILRINNASTVTTSTTGSIISAKGVGLNHVMNPQGLALDPAGNLFVADT
                     GNSRILRWANGNPAAGTAWALTGNTLGQVNRPEGVAIKFFQNGPLSGDLFLVVSDTSN
                     NRIQGRFLSAGPWNLVGVPNNIGSGVGQFRSPSKIR"
BASE COUNT          394 a          478 c          472 g          370 t
ORIGIN      
        1 ggggtaattg atgaaaagaa tgcaagatag tacccacccg aaatgatcag gattgccagc
       61 accgcaaccc cggtacgttt catttgattg cgtgtcggcc agccaaattc aaattggttt
      121 ccataggctg aaagcactcc cagcccacat cccaccgata gcggccattc ctgatacgcc
      181 agcccaaatg ccagaatcgc ggttaacatc aacccaagtt gggagaggtg aggtcgggag
      241 tgcataaggt ggcttttcgg gctgaagaca tcgggctgaa gaagtcgggc ctggggctaa
      301 ggggcttggg gcaatcgaag ggatgaggga tgagggatga gggatgaaaa aaaccagttc
      361 ttcagcccga aaatcttcag cccccagccc tcagcccaat atttccaacc caataccttc
      421 cgctctgtta tcggattttg gaggggcttc ggaattgccc gacgccactg ccgatgttgt
      481 ttggaactcc gaccagattc cacgggccgg cggagagaaa ccgaccctgg atccggttgt
      541 tgctggtatc actcaccacc aggaacaaat caccgctcaa tgggccattc tggaagaatt
      601 taatcgccac gccttctgga cgattgacct ggcccagagt atttccggtc aatgcccagg
      661 cggttccagc agccgggttg ccgttggccc accgcaggat gcgcgagttt ccagtatcgg
      721 cgacaaagag attccctgcc ggatcaagcg ccagaccttg tggattcatc acgtgattga
      781 gcccaacccc tttggccgag ataatcgaac cagtggtcga agtcgtcacc gtgctggcgt
      841 tgttgattcg cagaattcgg ctctgggctt catcggtgac aaacagccgg aacgtgccat
      901 caatcaccat tccgtgtggg ctcataatct gaccgcttgc ggttccattt gaggcaatca
      961 cgacgctgtt gcctggaaga ccagcttgaa accgcaccac tcgcccgttt ccattatctg
     1021 agacatagag atttccaagc gcgtcgtagg ccagaccttt tggtgaacga acctgattga
     1081 gcccggtgcc gttcgtggca aagttcgccc aggtgttgcc gccgtcggtt gaccattgaa
     1141 tgcgattgtt tccggtatcg gcgacataga tccgctgact atcggaactg gcaaccacgg
     1201 cttcaggtaa ccggaattga ccgtcacctg aaccgacggt tccgacacca accaccgacc
     1261 agttctggcc atcgaatcgc tgaatgcgat tattgttggt gtcagccacc cacaacacgg
     1321 tgtcgctccc gcagttgata aagaaaatgg tgaccgagtc actggccggg ccgcagacac
     1381 cggctggatc attggtcgtc aacgtcagtg tgaggctccc agactgcatt tcagccagtg
     1441 tggccatata gacagcgttg agcgcactgg cattgggcga aaatgatccg ctgccgccgc
     1501 tccaggtagc cgaagtggca ctgccgccga tacttccatt cagcgtgacc attggattgt
     1561 ctgaacacag cgtctgattg tttccggcat caaccgtgac gcgaggttca atggtaatga
     1621 ccacggtatc gaaaaccggg gcacaggcgc ccggcggatc attggtggtt aaactgagcg
     1681 ttgccgttcc agccgcaatt tcagccgctg atgg
//
LOCUS       DAOQDO010001601         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75355_length_1713_cov_2.431846, whole genome shotgun sequence.
ACCESSION   DAOQDO010001601 DAOQDO010000000
VERSION     DAOQDO010001601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75355_length_1713_cov_2.431846"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PKZ53_21105"
     CDS             <1..1325
                     /locus_tag="PKZ53_21105"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ42994.1"
                     /translation="IQAIYNASTGGKCKVPELVAAGATIITEGCNPGNGAIDPGETVT
                     VSLCVQNIGSSNTSALMGTLQATGGVTDIQSPNPQLYGVVIAGGSAVCAEFTFTATGT
                     CGDMLTASLQCQDGATDLGTLTYDFTLGTPGPTCCITPCTTCPSLYVADTSNNRVQMF
                     DGTAWSIIGVGSVGSGNGQFRLPEAVTFDVNNRIYVADTGNNRIQWSTDGGMTWANFA
                     TIGSGPNQVRAPQGLVIDAGGNLYVSDTGNGRVLRFDGGLPGNAVIIASNGTASGQVG
                     SPRGLAVDATFRLFVTDESNSRILRINNANTVVVSTSGVIIATVGSASNKVRNPQGVA
                     LDEDGTLYVADTGNSRILRWINANPNNSTTLALTGSGLGQVNHPEGITVSEFTTGPFA
                     GGPFLVVGDTSNNRIQGRFLPTGGWMLVGAPNGIGAGVGQFRSPSKIR"
BASE COUNT          396 a          429 c          468 g          420 t
ORIGIN      
        1 aaattcaggc catttataat gccagcactg gaggcaagtg caaagtcccc gaacttgtcg
       61 ccgccggtgc caccatcatc actgaaggct gtaaccctgg aaatggggcg attgatccgg
      121 gcgaaactgt aaccgtcagt ctatgtgttc aaaatatcgg gagttcaaat acttcagcgc
      181 tgatgggcac gttacaggcc acgggtggtg tgactgatat tcagtcgcca aatccacaac
      241 tctatggtgt cgtcattgct ggaggctctg ctgtttgtgc tgagtttact tttacggcaa
      301 ccgggacttg cggtgatatg ctgactgctt cgctccaatg ccaggatggt gccactgatt
      361 tgggaacgct gacctacgac tttacactgg ggacgccggg tccaacctgt tgcatcacgc
      421 cctgcaccac ctgccccagc ctgtatgtgg ccgataccag caataaccgg gttcaaatgt
      481 ttgacggaac tgcctggtcc attattggag ttgggtcggt tggttcaggc aatggtcagt
      541 ttcggttgcc cgaagctgtc acttttgacg tgaataatcg gatttatgtc gccgacaccg
      601 gcaataaccg cattcaatgg tcaaccgacg gcggaatgac ctgggccaat tttgccacaa
      661 ttgggtcagg tccaaatcag gtgcgggcgc cgcaggggct ggtcattgac gccggcggaa
      721 atttatatgt ttcagacacg gggaatggcc gggttctgcg ctttgatgga gggctgcctg
      781 gaaatgcggt gatcattgcc tccaacggta ccgccagcgg tcaggtcggc agtccacgtg
      841 gattggcggt ggatgccaca ttccggctgt ttgtcaccga tgaatccaac agtcgtattt
      901 tgcgcatcaa caacgccaac acggtggttg tttcgacttc cggcgtgatc attgccacgg
      961 ttggatccgc ctcaaacaaa gtcagaaatc cgcagggggt ggcccttgat gaagatggca
     1021 ctctctatgt ggccgatacg gggaattccc gaatcttgcg gtggatcaac gccaatccaa
     1081 acaacagcac aacgctggct ttgacgggtt ccggattagg ccaggtcaat caccctgaag
     1141 gcatcaccgt gagtgaattt accactggac cttttgccgg tggcccattc ctggtggtgg
     1201 gtgatacctc aaacaatcgc atccagggac ggtttctccc gactgggggc tggatgctgg
     1261 tcggagcacc gaacggcatc ggggcgggag tcggccaatt ccgatcaccg tcgaagatca
     1321 gataatgtgg aatgcggcgg cttgatgccg ctttgaggaa tgtgaaaagc ggtgacaagt
     1381 cgccgcattc caaagaattc tttgaaatgt ttttagggat gtgtcatatg gacgtgaaaa
     1441 cacctccatt cacgcaagtc cacaaaattg agccctctga atctcttacc aaaaacttga
     1501 agaacaaaga cgtggttgat tgagacagcc aaaaaaccga acttcaacca ggtgtaactg
     1561 accggcgtgc cttcatcttt ccggttgatt ggaattctgg ttctgtgtgt cctggctggt
     1621 ttgctcccaa acttaaaccc gattgccagt caggctttca atcaggcatc actgataatt
     1681 gatatagcct ttcagataaa tatttctgtt cag
//
LOCUS       DAOQDO010001602         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75393_length_1713_cov_1.889626, whole genome shotgun sequence.
ACCESSION   DAOQDO010001602 DAOQDO010000000
VERSION     DAOQDO010001602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75393_length_1713_cov_1.889626"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1713)
                     /locus_tag="PKZ53_21110"
     CDS             complement(<1..>1713)
                     /locus_tag="PKZ53_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42995.1"
                     /translation="NAVLLVRLANHYVWDIRDINGDGVPELYTSQATEEFPSMTLASA
                     LEVYAFSLSGSTGALQLYGKTLTQSQYLLRSVCIPDLPLIGASNETRIGLLMPNRAPF
                     FFSYFQQKRNGKVRLTTATPTPEGITVDLSLVRSGNVRTIWRDPVSGLLKFLVAEEIN
                     GALTGKVGFYEVESRRKALPAASSFVASPSSQLEPRVADLDGDGVNEILLRLPSRRIG
                     VFSCDPLTGTTQSLTTFEGTSVPLINELDIQSPTGNRPEILVTRNDAGRLRISALESS
                     GTFAAGTFAIRERWGVTFPEYPSDTALTLVTGKFLGAAQAAGVFISISRTGSLLLSGA
                     QGNTVWRTNEAFFFSNAVSVRDYDGDGDDDIYIVSNDKYSIRDGATGKALFGPVEVRT
                     LKGDLYSSAVLLPAGEVLLAGPARVVRIAKDNKRVWDFAKIIGTEAVMRINRAALPGV
                     APARFGSADVFIGANYGPEGTFSVYRMSDGVLVKRTGWWTSTDIVTVLGSREGDPTDG
                     LNWFVFGTADGQLVAVRADTGDTVWQLPLGAFATSPVIAQVGASKTLCLIFSTGDGQL
                     RVYPL"
BASE COUNT          421 a          489 c          459 g          344 t
ORIGIN      
        1 agcggataga cccgcaattg cccatcacca gttgaaaaaa tcaggcacag cgtttttgag
       61 gcgccaacct gggcaatgac aggtgaggtg gcaaaagcac caagcggaag ttgccagacg
      121 gtatcgccgg tgtcagcccg aacggccacc agttgcccat ccgcggtacc aaaaacaaac
      181 cagttgaggc cgtcggtcgg gtctccttcg cgcgagccaa gcacggtcac aatgtcggta
      241 ctggtccacc agccggtgcg tttgaccagg actccgtcac tcatccggta gaccgagaag
      301 gtgccttccg ggccataatt ggcgccgata aagacatccg ctgagccaaa tcgagcgggc
      361 gccacccctg gcaacgcagc gcggttgatg cgcataacgg cttcggttcc aatgattttt
      421 gcaaaatccc agacgcgttt gttgtctttg gcaatgcgga ccacacgggc tgggcccgcc
      481 agcaaaacct cgcctgctgg aagcaacacc gcagatgaat acagatcgcc tttcagtgtc
      541 cgaacctcaa cagggccaaa caatgctttc ccggtggcgc catcgcgaat actgtatttg
      601 tcatttgaaa caatatagat gtcatcgtcc ccatcaccat catagtcgcg aacactgacg
      661 gcattgctaa agaaaaaggc ttcgttggtg cgccagaccg tgttgccctg ggcaccgctg
      721 agcaacaaac tgccagttcg tgaaatactg ataaaaacac cggctgcctg tgccgcgccg
      781 agaaactttc cagtgaccag agtcagcgcc gtatctgatg gatattccgg aaatgtcacc
      841 ccccatcgct cacgaatagc aaaggttcca gccgcaaaag tcccacttga ttcaagggca
      901 ctgatccgca gacgtccggc atcattgcgg gtgaccagga tttccggtcg gttgccggtg
      961 ggtgactgaa tgtcgagttc attgatcaat ggaactgacg tgccttcaaa tgtggtcaga
     1021 ctctgggtgg ttcccgtcag cggatcgcag ctaaacacgc cgatacgccg tgagggcaat
     1081 cgcagcagga tttcattgac cccatcgcca tccagatcgg ccacgcgtgg ttcaagttgc
     1141 gacgaaggcg aggccacaaa cgaagaagcg gctggcagcg ccttgcggcg gctttcaacc
     1201 tcataaaacc cgactttgcc ggtcaaggcg ccgttgattt cttcagccac cagaaacttg
     1261 agcaacccgg atactggatc acgccagatc gtccgaacat tgcctgaccg aaccagcgat
     1321 aaatcaacgg taatgccttc gggcgtcggc gtggctgtgg tcaacctgac ttttccgttt
     1381 cgtttttgct gaaaataaga aaagaagaat ggggcccgat ttggcatcag gagaccaatt
     1441 cgggtttcat ttgaggcgcc aatcaacggc aaatccggga tgcacacact gcgcagcaaa
     1501 tactgtgact gggtcagggt ttttccataa agctgcaggg cgccggtgct tccactgagc
     1561 gaaaaagcat agacctccag tgccgaagcc agcgtcatcg aggggaattc ttcggtggcc
     1621 tgtgaggtat acagttccgg aaccccatca ccgttgatat cccggatatc ccacacataa
     1681 tggttggcca gccggacaag caacaccgcg ttc
//
LOCUS       DAOQDO010001603         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75433_length_1712_cov_2.831623, whole genome shotgun sequence.
ACCESSION   DAOQDO010001603 DAOQDO010000000
VERSION     DAOQDO010001603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75433_length_1712_cov_2.831623"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PKZ53_21115"
     CDS             complement(<1..1308)
                     /locus_tag="PKZ53_21115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNJ42996.1"
                     /translation="MFFYGRLTTFTWRLSRTWLLLATLVVGFSFGISAQTPQIVAGTV
                     VDTRKVPIADATVIIRTTAGEQSVTTDKQGHFRCEIAQAVTAVVVEGKNLIRVEKSPS
                     PGENVENLEIEADYVVSPVHDTIVIKGDTTEPTINRQDDTLYREGFPARDDQLFFQFG
                     SGINAGQHEGGGKSLEIRRFGFNLDHGGVGPGLLVRVDNFDQNQATQGHGQGYLGQLK
                     SLSSELVDNVQILNGPFSAQYGNFSGLGVVQVSLKESLAETAIVRLQGGSFNTKRGFF
                     AVSPKNGFLAYELTRTDTPFDKPYDRDNLTGAYKVDLGAEKSLAFKFNFGRNDFFSSG
                     QLPTDLIASRDLDRFGAIDGTEGGRVRTFNGSVLFKKEFQDGSSLKLDGLMARSLFDL
                     YNNFTFFLENETLGDQFNQHDSRLQQSANIQYLKPYKIGGYDAV"
     gene            complement(1515..>1712)
                     /locus_tag="PKZ53_21120"
     CDS             complement(1515..>1712)
                     /locus_tag="PKZ53_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ42997.1"
                     /translation="KIHLDETKNVDEPVGRFIFVNTHHTHNLTHQIPNGSTATAWESS
                     TPIFPLTITVAETSPFAPHCG"
BASE COUNT          424 a          439 c          434 g          415 t
ORIGIN      
        1 caccgcatcg tatccaccga ttttataggg cttcaaatat tgaatgttgg cgctttgctg
       61 caacctcgaa tcatgctggt tgaactgatc accaagagtt tcattttcca ggaaaaacgt
      121 gaaattgttg tagaggtcaa agagtgaccg ggccatcaag ccgtcaagtt tcaaggacga
      181 tccatcctgg aactctttct tgaacaacac cgaaccgttg aaggtccgca cccgaccacc
      241 ttctgtgccg tcaatggccc caaaacggtc caaatcgcgg ctggcaatca ggtcggttgg
      301 aagctgaccg gatgaaaaaa agtcgttccg gccaaaattg aacttgaacg ccaggctttt
      361 ttccgctccc aggtcaactt tgtaggcgcc ggtgaggttg tcgcggtcat agggtttatc
      421 aaatggggta tcagtgcggg tcagttcata ggccaaaaat ccgttcttcg ggctgaccgc
      481 aaaaaatcca cgcttggtgt taaacgagcc gccctgcaac cggacaatgg cggtttctgc
      541 cagggattcc ttgagtgaaa cctggaccac gcccagaccc gaaaaattgc catattgggc
      601 cgaaaatggg ccgttcagga tttgcacatt atcaaccagc tcactggaaa gcgatttgag
      661 ttgccccaaa tacccctggc cgtggccctg ggtggcctgg ttctggtcaa aattatctac
      721 ccgcacgagt aaccccggtc caacgccgcc atggtccaga ttgaatccaa agcgacggat
      781 ttcgaggctt ttccctccgc cttcgtgctg gccggcattg atgccactac cgaattggaa
      841 aaacagttgg tcatcgcgag ccggaaagcc ttcacggtac aaggtatcat cctggcgatt
      901 gatggtcggc tcggtcgtat cgcctttgat aacaatcgtg tcgtgaaccg gagacacgac
      961 atagtcggct tcgatttcga ggttctcgac gttttcgccc ggactgggcg atttttcaac
     1021 ccgtatcaga tttttccctt ctactaccac cgctgtcact gcctgtgcaa tctcacaccg
     1081 gaaatgacct tgcttgtcag ttgtcaccga ttgctcgcca gcggtggtac ggatgataac
     1141 cgtagcatca gcaatcggta ctttgcgggt gtcaaccact gtcccagcaa ctatttgcgg
     1201 agtttgggct gaaattccga aagagaaacc gaccaccagc gttgccagca gcagccaggt
     1261 gcgtgagagt cgccaggtga aggtggtgag tcttccataa aagaacataa tcgaaatcct
     1321 tcatacacga aaatgtgtta caaaattaaa tttcgtagca cgaattataa caaaaactgg
     1381 ctgtcctgca gccaaaaaca ttttcctgtc tgaaaaaaac tacggcggct caatctcgtt
     1441 gcttgacggg tatcaataaa atcccgtggt gaatggcctt gagcagtcaa aatgaatcgg
     1501 ccaacgccca atcctcagcc gcagtgcggt gcaaaaggac tggtttcagc aacggtgatc
     1561 gtcaagggaa agatcggtgt tgaggactcc caggccgtgg cggtgctgcc gttcggtatc
     1621 tggtgcgtca ggttgtgagt gtgatgagtg ttgacaaaga tgaacctgcc tacgggctca
     1681 tccacatttt tggtttcatc cagatgtatt tt
//
LOCUS       DAOQDO010001604         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75437_length_1712_cov_2.748340, whole genome shotgun sequence.
ACCESSION   DAOQDO010001604 DAOQDO010000000
VERSION     DAOQDO010001604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75437_length_1712_cov_2.748340"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1712
                     /gene="alaS"
                     /locus_tag="PKZ53_21125"
     CDS             52..>1712
                     /gene="alaS"
                     /locus_tag="PKZ53_21125"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNJ42998.1"
                     /translation="MGFTARHNTFFEMLGNFSFGDYFKEEAIVFAWDLLLKEFGLPLE
                     RLWFTVYEDDDEAIELWRKVGAPTNRILRFGKKDNFWQMGDIGPCGPCSEIHYFQGED
                     MADNLPEYVNGPGDTTVEIWNLVFMQYDRSADGTLTPLPKPSVDTGAGLERVAAVLQG
                     KPSNYETDLIRPIIDFIASLAGKDYAYDMEDDHVSMRVISDHARTTAFAMADGIYPGN
                     KGRNYVLRKIMRRAIWHGRRLGMEDLFLYKVTNFVIDHMAGAYPELRTQANEVERATL
                     TEEKLFSSTLNNGTKKLNEIFARSDSGVVSGQDAFTLYDTYGLPIDMTVFIAEQNKMT
                     VDVEGFEEILKGRKKADRESWTGAIAQVKEVYQSVHNGIPTVFTGYEGTALAETKIRG
                     LICEDQVVEVLKAGETGEIILERTPFYAESGGQVGDTGLIESISATARVSNTYAPVSG
                     LTAHKVTIETGEFKVGDLVSATVDAQRRQRIVLNHSATHLLHAALKEVLGPHVKQAGS
                     EVAPDRLRFDFTHFAPLTQEELTEIERLVNNEIRRNTGVSKIEMDL"
BASE COUNT          454 a          430 c          453 g          375 t
ORIGIN      
        1 acggcgcaaa aatgcttacg cgctggtggc aaacacaacg atttagaaaa tgtgggattc
       61 actgcgcgac ataatacttt tttcgaaatg ttgggtaatt tttcctttgg cgactatttc
      121 aaagaggaag ccatcgtttt tgcctgggac ctgctcctga aggaatttgg cctcccactc
      181 gaacggctgt ggttcaccgt gtatgaagac gacgacgaag ccattgaact ctggcgcaaa
      241 gtcggcgcgc cgacaaatcg aattttgcga tttgggaaaa aagataactt ctggcaaatg
      301 ggcgacatcg ggccctgtgg cccctgctcg gaaattcact atttccaggg cgaagatatg
      361 gccgacaacc tcccggaata tgtcaacggc cccggtgaca caacggttga aatctggaat
      421 ctggtcttta tgcagtatga tcggtcagcc gatggaacac tgaccccgct gccgaaaccc
      481 tcggttgata ccggtgccgg gctggaacgg gtcgcagccg tacttcaggg caaaccatcc
      541 aattatgaaa ctgacctgat tcgacccatt attgacttca ttgccagtct ggctggaaaa
      601 gactatgcct atgacatgga agacgaccat gtttcgatgc gggtgatttc cgaccacgcc
      661 cgaacaacag cctttgccat ggcagatggg atttatcctg gaaataaggg tcgcaactat
      721 gtgctccgca aaattatgcg ccgagccatc tggcacgggc ggcggctcgg gatggaggac
      781 ttgtttctct ataaagtcac caactttgtg attgaccaca tggcgggtgc ctaccccgaa
      841 ttaagaacgc aggccaatga agttgaacgc gcaaccctga ccgaagagaa actgtttagc
      901 tcaaccctga acaacggcac gaaaaaactc aatgaaatct ttgcgcggag tgacagtgga
      961 gttgtctctg gccaggatgc cttcacactc tatgacacct atggtctgcc gattgatatg
     1021 accgtcttca ttgccgaaca aaacaagatg acggttgatg tcgaaggatt tgaagaaatc
     1081 ctgaaaggcc ggaaaaaagc agatcgcgaa agctggacag gcgcaatagc tcaagtcaaa
     1141 gaagtctatc aatcagtcca caatggcatt ccaacagtct ttaccggcta tgaaggcacg
     1201 gccctggcgg aaactaaaat tcgggggttg atttgtgaag atcaggtggt tgaggtgttg
     1261 aaggcaggcg aaactggcga aatcatcctt gaacggacgc cgttttacgc cgagtcgggc
     1321 ggccaggttg gcgataccgg gttgattgaa agcatttcgg caaccgcccg ggtcagcaat
     1381 acctacgctc cagtttcggg cctgaccgca cataaggtca ccattgaaac tggcgaattc
     1441 aaggtcggag acctggtttc ggccacggtt gacgcgcaac gtcgccaacg aatcgtcctc
     1501 aaccactcgg caacacatct gttgcatgcg gccttgaagg aagtgcttgg gccgcacgtc
     1561 aaacaggctg gaagcgaagt ggcgccagac cggctccggt ttgactttac ccattttgcc
     1621 ccacttaccc aggaagaact cactgaaatt gagcggctcg taaacaatga aatccgacgc
     1681 aataccgggg tttcaaaaat cgaaatggac ct
//
LOCUS       DAOQDO010001605         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75610_length_1710_cov_4.043505, whole genome shotgun sequence.
ACCESSION   DAOQDO010001605 DAOQDO010000000
VERSION     DAOQDO010001605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75610_length_1710_cov_4.043505"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            611..>1710
                     /locus_tag="PKZ53_21130"
     CDS             611..>1710
                     /locus_tag="PKZ53_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNJ42999.1"
                     /translation="MAEPTKGLQDIVAADSTICLITPDGRLAYRGLDIEDLAEHSTFE
                     ETVFFLLYGHLPKQAELDDFKVKLAEGRKLPAEIITLMKDFPKTAFPMEALRTATSAL
                     SFYDPDTHDNSYEASVRKAIRLIGQLTLLTTGFDRIRRGLEPIEPDPSMSMAEAFLYS
                     LHGEKPSASGARAFDVCLILHADHELNASTFAARVIASTLSDVHAAITGGIGALAGPL
                     HGGANQRVLEMLLDIGELDRVEAYVDDIFTNKKKIMGFGHRVYRATDPRANIFRVFSK
                     NLGEETGEPKWFQMCEMIEKMVFEKKKLYPNVDFFSGTVLHALKIPTDLFTPVFACSR
                     VSGWSAHVLEQLNNNRLIRPVADYQGAIKMEYT"
BASE COUNT          421 a          421 c          408 g          460 t
ORIGIN      
        1 atgaagaatg aagaatgaag aatgaagaat gaagaatgaa gaatgaagaa tgaacagtta
       61 ctgataggtg agcttctatc tcgatagggt cacgtcaggt tgatgttgga aataaaaggg
      121 ttgtttgtgc cgattgtgtg aacgaataga cgatttgctg cctgtaacct atcttatggg
      181 aagcagttaa cctcaataag atccgggctt catcttcaat tttttattct tcattcttca
      241 ttctcaattc tcaattctca attcttcata ctcaattctt cattcttcat tccatcggaa
      301 actcttcagt cagttgcgtt gacacgtctt tgacggcttt gttatgctcg ctcggctttt
      361 atcctcacca accataaagc tatcacaatc tttctaaagg ctttcctggc aaggggacac
      421 gtttttttca tcaatcctaa ccacgctccc cactgcggtg taacaacctg gtttcttcca
      481 cgagagattg tgccggcagg cggtgaggta tctggttgtt ttccggacta ttatcctgtg
      541 ccatgggcgt cagatgtcgc ccccaacttc gagttgatat ggcttcatac ttcatcatag
      601 gagatcctca atggcagaac ctaccaaagg cttacaagac attgttgccg ccgattccac
      661 aatctgtttg attacaccag atggacggct ggcttatcgt ggactcgaca tcgaagattt
      721 agcggaacac tcaacctttg aggaaaccgt ctttttcctg ctctatggcc atttgccaaa
      781 gcaggctgaa ctggatgact tcaaagtgaa gttagccgaa ggtcggaaac ttccggcgga
      841 aatcatcacc ttgatgaaag atttcccaaa gactgctttc ccaatggaag ccctgcggac
      901 ggccacctca gccctctcgt tttatgaccc ggacacgcat gacaattcct atgaagcgag
      961 cgtacgcaaa gccattcgtc tgattggtca gttgacgttg ctcaccaccg ggtttgaccg
     1021 gattcgccgc ggactcgaac caattgaacc agacccgtct atgtcgatgg ccgaagcgtt
     1081 cctgtactca ctgcacggcg aaaaaccatc agcttcaggt gcccgtgcct ttgacgtctg
     1141 cctgattttg catgccgacc acgaactcaa tgcttcgacg tttgctgcgc gggtgattgc
     1201 ttcaacgttg tcggacgttc acgcggcaat tacgggtgga attggcgccc tggctggacc
     1261 attgcacggc ggcgcaaacc agcgcgtgct ggaaatgctg cttgatattg gtgaactgga
     1321 tcgtgtggaa gcctatgtgg atgacatctt taccaataag aagaagatca tgggctttgg
     1381 tcatcgcgtt tacagagcaa ctgaccctcg ggccaacatt ttccgcgtct tctcaaagaa
     1441 tttaggcgaa gaaaccggcg aaccgaaatg gttccagatg tgcgaaatga tcgaaaaaat
     1501 ggtttttgag aagaagaagc tgtacccgaa tgtggacttc ttctccggca ctgtgttgca
     1561 cgcgctcaag attccgaccg atctctttac cccggtgttt gcttgcagcc gggtttcagg
     1621 ctggtcggca cacgtactgg aacaactcaa caacaatcgt ttgattcgcc cggtggctga
     1681 ttaccagggc gcgatcaaga tggaatacac
//
LOCUS       DAOQDO010001606         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75630_length_1710_cov_2.666465, whole genome shotgun sequence.
ACCESSION   DAOQDO010001606 DAOQDO010000000
VERSION     DAOQDO010001606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75630_length_1710_cov_2.666465"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKZ53_21135"
     CDS             complement(<1..840)
                     /locus_tag="PKZ53_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43000.1"
                     /translation="MNIRLPRPKHWLWAVVLILLGCVSPLQAQEKLPAQTWNDPQLKP
                     SDPLTPMNNAFRVAYAGLRTRLLAETSPVIVHAGDKLILFRNGVQTDVATFTPRYHEL
                     KSVAHVPLALYVMLVHDAGSKLTAEQLTQLRDYRALVVAGRDSIAGRNFASEQQTRQY
                     RLLDQALALIDAILKDGMISRNALRQYTKSQAQDVLANAYDAADDQLATMDAQVKAWL
                     AQMTPEERKRVKIAVGSAHMPRLGNLAMQYFSATFNEPYEGKFEIEEEENSDFRVIYA
                     ESLF"
     gene            complement(928..>1710)
                     /locus_tag="PKZ53_21140"
     CDS             complement(928..>1710)
                     /locus_tag="PKZ53_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43001.1"
                     /translation="PDWIVQQHRQWFDEFINHGDEIGLHTHAWRWDEQFEVWIADHAN
                     QPWIEHCLQVSFDAFYEAFGRSCLSFRFGDRWMNNPTTALIQKLGVLYDLTLEPGHTT
                     TPALVRDECYTGAIPDYTRVPRLPFRPTTQDFRQSDPSRTEGMRMIPLSSGFHRKRHW
                     VFDHIPVKYLPSSHRRAVTLGLWHKPEILFPILNHLLNIEQSPYLTLVLRSDLPIIPL
                     ANNLHRCIEHLLNHPRIQDFVFSTPAETMTHIGLRDQRGFAG"
BASE COUNT          387 a          426 c          489 g          408 t
ORIGIN      
        1 aaagagggat tcggcataaa tcacccggaa atcgctgttc tcttcttctt cgatttcaaa
       61 tttgccttca tagggttcgt tgaaggtggc cgaaaaatac tgcatagcca gattcccaag
      121 tcgtggcata tgggccgagc caacggcaat tttcacccgt ttgcgttctt cgggtgtcat
      181 ttgagccagc caggctttga cctgtgcgtc catggtggca agctggtcat cggcagcgtc
      241 ataggcattg gcaagcacat cctgagcctg acttttggtg tattggcgca gggcattacg
      301 gctgatcatt ccatctttca gtatggcatc aatcaacgcc agtgcctgat ccagcaatcg
      361 gtactgccgg gtttgttgtt cagaagcgaa gttgcgcccg gcgattgagt cgcgccctgc
      421 caccaccagt gcccggtagt cgcgcaactg ggtgagttgc tccgccgtca gttttgagcc
      481 cgcatcatga acaagcatca catagagcgc cagcgggacg tgggccacag atttgagttc
      541 gtgatagcgc ggcgtgaagg ttgccacatc agtttgaacc ccgtttctaa acaggatcaa
      601 tttgtctccg gcatggacaa tgaccgggct ggtttccgcc agcagtcgag ttcgcaaacc
      661 ggcataggcg acccgaaagg cgttgttcat cggcgtcagc gggtcagacg gcttgagttg
      721 tgggtcattc caggtttgag ccggcagttt ctcctgggcc tgaagcggtg agacacaccc
      781 gagcaggatg agaacgacgg cccaaagcca gtgctttggc cggggcaggc ggatattcat
      841 tggctgatct cctgagaact aagttgaagt ggaaattgaa aaactatgcg gatgcgggtg
      901 catcttgcca ctggtgtgag tgctcattca accagcaaac cctcgctggt ccctcagtcc
      961 aatgtgggtc atggtttcag ccggcgtgga aaacacaaaa tcttggattc gtgggtggtt
     1021 cagcaggtgt tcaatacatc ggtgcaggtt gttggccagt ggaattatgg gcagatcaga
     1081 ccgcaatacc agcgtcagat agggagactg ctcgatgttc agtaaatgat tcaagatcgg
     1141 gaacagaatt tctggtttgt gccacagtcc cagcgtgacg gcccttcggt gtgaactcgg
     1201 gaggtacttg actggaatgt ggtcgaaaac ccaatggcgc tttctgtgaa agccgctgct
     1261 gagcggaatc atccgcattc cctcggttcg agatgggtca gattgacgaa aatcctgagt
     1321 tgtgggccgg aacggcaagc gtgggacacg ggtgtaatcc gggattgccc cggtgtagca
     1381 ctcatccctg accagcgccg gggttgttgt gtggcctggt tccagtgtca ggtcatacaa
     1441 cacaccaagt ttctggatca aggctgtggt cgggttgttc atccagcgat caccaaaccg
     1501 gaagctaagg caacttcttc caaatgcttc ataaaacgca tcaaaagaaa cctgaagaca
     1561 atgttcaatc cagggttggt tggcatggtc agcaatccag acttcgaatt gttcatccca
     1621 ccgccaggcg tgcgtgtgga ggccaatttc atcaccgtgg ttgataaatt cgtcaaacca
     1681 ctgccgatgt tgctgcacaa tccaatcagg
//
LOCUS       DAOQDO010001607         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75680_length_1709_cov_4.189238, whole genome shotgun sequence.
ACCESSION   DAOQDO010001607 DAOQDO010000000
VERSION     DAOQDO010001607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75680_length_1709_cov_4.189238"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PKZ53_21145"
     CDS             complement(<1..814)
                     /locus_tag="PKZ53_21145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43002.1"
                     /translation="MDDNSAESLVGLVLNGKYRLERHIASGGMASIYAATRLEIGDTV
                     AVKILTPDALRNPMAVLRFRRESQAAARIKHPGVVSVYDFSTLPDGRTFMVMEFLKGQ
                     SLRDELTTCGRIAPERVLAVMEQVCAAMQSAHEAGIIHRDLKPENIMAQLNSDGTETF
                     KVVDFGVAELREQAVGTALTKLTEAGMMVGTPYYMSPEQCRSQYLDARSDIYSLGIIL
                     YEMLTGTVPFASRTLSAVIIQQVTEPPRPLRDHRPEISPALERVVLKALAKEC"
     gene            complement(1092..1541)
                     /locus_tag="PKZ53_21150"
     CDS             complement(1092..1541)
                     /locus_tag="PKZ53_21150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNJ43003.1"
                     /translation="MHPRNPDFRSKLEHIFGKAPFISELGVQVTDCGAGWCETSLLVQ
                     PKHYQQDTFVHAGVQSTMADHTAGGAAGTLIGPDETILTANFFINLLRPAIGTRLRCK
                     ATVLKPGKTLIVVESEVYTGTEHHEKLTAKATVTLAVIKLTSLSAST"
BASE COUNT          395 a          422 c          432 g          460 t
ORIGIN      
        1 cacactcttt ggccagcgct ttgagcacca cccgttccag cgccggagaa atttcaggcc
       61 gatggtcacg taagggacgc ggaggctcag tgacctgctg aatgatcacg gcggaaagcg
      121 ttcgcgaggc aaagggcacg gtcccggtca acatttcata aagaatgatc cccagcgaat
      181 aaatatctga ccgtgcatcc agatactgtg accggcattg ttcaggcgac atgtagtagg
      241 gagtcccaac catcatcccg gcttcggtca gtttcgtcag tgccgtcccc acggcctgtt
      301 cccgaagttc agcaacccca aagtctacga ctttaaaggt ttcggttcca tctgagttga
      361 gctgagccat gatattttct ggcttgagat cacgatggat aattccagct tcgtgggctg
      421 actgcatcgc cgcacagacc tgctccatca cggccagcac ccgctctgga gcaattcggc
      481 cacaggtcgt gagttcatcc cggagcgact gccctttgag aaactccatc accataaagg
      541 tgcgaccatc gggcagtgtg ctaaagtcat agaccgaaac cacaccgggg tgcttgatgc
      601 gcgccgctgc ctgcgattca cgccggaacc gaagcaccgc catcggatta cgaagcgcat
      661 ccggggtcaa aatcttaacc gccaccgtat cgccaatttc cagccgtgtt gccgcataaa
      721 tactggccat gccgcccgag gcaatgtggc gttccagacg atatttgcca ttgagcacca
      781 atccaaccag actctcagcc gaattatcat ccaaccgaat gccgtccttt ggacagataa
      841 gaaattcctg tggatatgat tcctggcaaa cctggcaata cttcatgagc gccttgatcg
      901 aatagaaaat gtagaatgag tgcttggtgc ttggttcttg gttcttggtt cttggttctt
      961 ggtgcttggt tcttggttct tggttcttgg ttcttagttc ttggttctta gttcttggtg
     1021 cttggtgctt ggtgcttggt tttttggttc ttcttggaat gtactggttc ctaagcacca
     1081 ggtaccaagc actaagtact ggctgacaag gatgtcagtt tgatcacagc cagtgtgacc
     1141 gtggctttcg cggtgagttt ttcgtgatgt tcggtgccag tataaacttc tgattcaacc
     1201 acaatcaggg ttttgcctgg tttgagaaca gtggctttgc atcgcagacg agtgccgatg
     1261 gctggccgca gcaggttgat gaaaaaattt gcggtcaaga tcgtttcgtc tggaccaatc
     1321 agagttccag ctgctccacc agccgtgtga tcagccattg ttgactgaac tccagcatga
     1381 acaaatgtat cttgttgata gtgctttggt tgaaccagta aactcgtctc acaccagcca
     1441 gcgccgcaat ctgtgacttg aactccaagc tcagaaataa aaggtgcttt tccaaaaata
     1501 tgttcaagtt tgctgcgaaa atcggggttc ctggggtgca tgatttttta tctgtatgca
     1561 ggtgtaaggt aagaaacgat agctgtcgca gtttccctta aaaaggcgga aggccgcaag
     1621 atatttcctt cctgatgagg ttcaatctta tattagtttg tagtcagtag ccagtagtca
     1681 gtagttcact aagtttattt gattgaact
//
LOCUS       DAOQDO010001608         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75688_length_1709_cov_3.451028, whole genome shotgun sequence.
ACCESSION   DAOQDO010001608 DAOQDO010000000
VERSION     DAOQDO010001608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75688_length_1709_cov_3.451028"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..>1709
                     /locus_tag="PKZ53_21155"
     CDS             237..>1709
                     /locus_tag="PKZ53_21155"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ43004.1"
                     /translation="MNHARCFPKSAVVRQTLGSLAALTLCLLLTTVQTVAAKLPPAPN
                     VEKVKLQELKRHLSFLSSPEVGGRFTFSVGNRVAARYLASQLEFFGFQGAMPDKSFFQ
                     VVPFTRREVDLAKTRLTLGNNPTAFHYLDDFIVYEQTCPQKVEATADLVFVGYGYSSP
                     ENGYDDYQGLDVKGKIVVTIPFGTPKVLEGKTLPNSAIGVGAAEAHGAVGVITIPDSF
                     SEDIWKAIKAGAAMYGGRPSLKLTIPGNGDPLPRIPNILAGPKLATSLMDLLGMKYDD
                     LRKLADVGDTIKPQVLNTSCQASIVYPEILIDQARNVVGILEGSDPKLKNEYIVLSAH
                     YDHLEGNEKRYFPGADDDGSGTSAVLELARVLGAERPRRSVIVLFNTGEEIGLLGSEY
                     FVENSPVPLSQIVANFNIDMIGRSRAEGDTKQENSKLTDANSVYLIGPDKHSTELFAI
                     SEVTNQEITKFTIDYTYNNENDPSRFFYRSDHWNFAKKGIP"
BASE COUNT          429 a          419 c          444 g          417 t
ORIGIN      
        1 attttgataa gccctttgtt ttcaacgaac aattttttca acccttaact ggcattattg
       61 gttcctaagt accaggtacc aggtaccaag taccaggtac caagcaccaa aaaccaggaa
      121 cgaaattgaa agaaaccgca aatgaatggt cgttgtgtgc taagctgagg cttttccaaa
      181 gccgtcccgg ccatcatact ccgtcgcaca cttgaacaag aaggaaaatc aattggatga
      241 atcacgcgcg ttgtttcccc aaatcagccg ttgtgcgcca aacgctcggt tcgttggctg
      301 cactcactct gtgtcttttg ctgacgacgg ttcaaaccgt cgctgccaag ctcccgccag
      361 cacccaacgt cgagaaagtc aaattgcagg aactcaagcg ccatttgagc tttttgtctt
      421 cacctgaagt cggtggccgc tttacgttta gcgttggaaa tcgggttgca gcccggtatc
      481 tggcctcaca acttgaattt tttggatttc aaggagcgat gcccgacaaa agcttctttc
      541 aggttgtgcc gtttacccgg cgggaagttg atctggcaaa aacccggctg acgttgggca
      601 acaacccgac ggcctttcac tacctggatg atttcattgt ctatgagcag acctgtccgc
      661 aaaaagtaga agccaccgcg gatctggtct ttgtcgggta tgggtactca agccccgaaa
      721 atggctatga cgattatcaa gggcttgatg tgaagggcaa aatcgttgtc acgattccgt
      781 ttggaacgcc taaagtattg gaagggaaaa ccttacccaa tagtgcgatt ggggttgggg
      841 cggctgaagc ccacggcgcg gttggggtca ttacgattcc agattccttt tccgaagata
      901 tctggaaagc gatcaaagcc ggggccgcga tgtacggcgg gcggccttcg ctcaagctga
      961 cgattccagg caatggggac cctcttccga gaattcccaa tattctggcg ggtcccaaac
     1021 tggcgacgtc gctgatggac ctgctgggga tgaagtatga cgatttgcgc aagctggcgg
     1081 atgtgggcga tacgatcaaa cctcaggtgc tcaatacatc gtgccaggcc agcattgtct
     1141 atcccgagat tttgattgat caggctcgca atgtcgttgg gattttggaa ggcagcgacc
     1201 caaagctcaa aaacgaatat atcgtgttga gtgctcacta tgatcatctg gaaggcaatg
     1261 aaaaacgata tttcccaggg gctgatgatg atgggtctgg gacttcggcg gtgctggaac
     1321 tggcccgggt cctcggtgcc gagcgtcctc gtcggtcagt gattgtgctc tttaacaccg
     1381 gcgaagaaat cggcctgctg gggtcagagt attttgtcga aaactcacct gtgcctctgt
     1441 cgcagattgt ggccaatttc aacattgata tgattggccg ttcacgggct gaaggtgaca
     1501 ccaagcagga gaattcgaaa ctgacggatg ccaattcggt ctatctgatt ggcccggata
     1561 aacacagtac cgaactcttt gccatcagcg aagtgacaaa ccaggaaata acgaaattca
     1621 cgattgatta cacttacaac aacgaaaatg acccctcccg gtttttctac cggagtgatc
     1681 actggaattt tgccaaaaaa ggcattccc
//
LOCUS       DAOQDO010001609         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75781_length_1708_cov_2.684815, whole genome shotgun sequence.
ACCESSION   DAOQDO010001609 DAOQDO010000000
VERSION     DAOQDO010001609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75781_length_1708_cov_2.684815"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PKZ53_21160"
     CDS             complement(<1..951)
                     /locus_tag="PKZ53_21160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43005.1"
                     /translation="MHQVIRDLRTSLRFFVRKPGFCTIIVLTLALGIGANTAIFSVLH
                     AVLINPLPYREPDRIVGLRESLPDEGIIPVSYRSFAEWRDRLTTFERVAAGSNWNPNL
                     EDEHNPLRLIGATVSGGYFDVMGLSPLVGRVPMADDERPDAPRVVVLGEQLWRNRFHR
                     SPTVIGQQIRLSGNQFTVIGVVPDPFVQTNIGSVELWTPMRVNDQTARENPGRYLFVV
                     GRLKPGITLEQARSDAARVMTLIKQEFPETHNSRYQSVVQPLRDFVVSRDMQQALWLL
                     VLAVDCLLLIAGSNVAGLLLAHVTQRERELAIRAALGASRW"
     gene            complement(1325..>1708)
                     /locus_tag="PKZ53_21165"
     CDS             complement(1325..>1708)
                     /locus_tag="PKZ53_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43006.1"
                     /translation="GQGTTPTVIRKKNAFTVISDTAQAEIPFTLEFEAPTATVVRGKV
                     IDTVLHIKRNEGFMEAVTISISDDPLNRIRISPSTLTSTGCCLPVSISTRGIGPTGTF
                     TVNVSGKDSSGRVRTATLELNMKSE"
BASE COUNT          423 a          444 c          463 g          378 t
ORIGIN      
        1 ccaccgactg gcgccaagtg cggcccggat ggccagttcg cgttcgcgtt gagtgacgtg
       61 cgccagcaac aacccggcga catttgagcc ggcaatcagg agcaagcaat ccacggccag
      121 caccagtagc cagagcgcct gttgcatatc tcgggatacc acaaaatcgc gaagtggttg
      181 aaccactgac tgatagcggc tgttgtgggt ttctggaaat tcctgtttga tcagggtcat
      241 gactctggcg gcatctgatc tggcctgttc cagcgtgatt ccaggcttga gccgcccaac
      301 cacaaacaag taccggccag gattttcacg ggcggtctgg tcattcaccc gcatgggcgt
      361 ccagagttca actgagccga tgttggtctg aacaaacggg tcaggcacaa ccccaatcac
      421 ggtaaactga ttcccactca accggatctg ctggccgatg actgttggcg aacgatggaa
      481 ccgattccgc cagagttgtt cgcccagcac aaccacccga ggggcatcag ggcgttcgtc
      541 atcagccatt ggaacccgcc ccaccagcgg agaaagcccc ataacgtcaa agtacccgcc
      601 tgacacggtg gcgcctatga gtcgaagcgg gttgtgctcg tcttccaggt ttggattcca
      661 gttggatcca gcggccactc gttcaaaggt agttaagcgg tcgcgccact cggcaaatga
      721 acgataggaa accggaatga tgccttcatc cgggagggat tcgcgcagac caacaatgcg
      781 gtcgggttcg cgatagggaa ggggattgat caaaactgcg tgtaaaacac tgaaaatcgc
      841 cgtgttggcg ccaattccca gcgccagggt caacacaatg atggtgcaga atccaggctt
      901 gcgcacaaaa aaccgcaggc tggtccgcag atcacgaatt acttgatgca cgacataact
      961 ccagaaaatg aaagatggct tgaatgaaaa aaggcgaatc tgggtcagaa gtgcaattgt
     1021 tattccagaa aacaaagcgt gatattttct ggggtgcgcc aacttgtcgg cgctttcgat
     1081 cctggcgact tgccgccagt caaccgaagt aatgccattt agggttcagg gtatcgggtt
     1141 cagggttcag ggaaatacag ttttttcaac gggttagtct tcctgtaata caaggctttc
     1201 attccaaaat ggtattatcc agttatgatg taccggggtc tgaaaaagac cagacggaca
     1261 gtttggaagg ggcgactggt ttgaactgcg cccaattcga aaacccggaa cccggaaccc
     1321 ggcttcactc gcttttcata tttaactcaa gcgttgcagt tcgaacccgc cctgagctgt
     1381 ccttgcccga aacattcacg gtgaaggtcc cggtcggtcc aatcccccgc gttgaaatcg
     1441 aaaccggcag acagcaacca gtgctggtca aggtcgaagg cgaaattcta attctgttca
     1501 acggatcatc actgatagaa atggtcacgg cttccataaa tccttcattg cgtttgatat
     1561 ggaggaccgt atcaatgact ttcccgcgaa caaccgtggc ggttggcgct tcaaattcga
     1621 gtgtgaatgg aatttcagcc tgggcagtat ctgatatgac ggtgaaggca ttctttttgc
     1681 gaatgacagt tggggttgtt ccttgccc
//
LOCUS       DAOQDO010001610         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75802_length_1708_cov_2.308530, whole genome shotgun sequence.
ACCESSION   DAOQDO010001610 DAOQDO010000000
VERSION     DAOQDO010001610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75802_length_1708_cov_2.308530"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /locus_tag="PKZ53_21170"
     CDS             complement(<1..>1708)
                     /locus_tag="PKZ53_21170"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNJ43007.1"
                     /translation="VVDSLGQVTVAGITESTDFPVHNARQAVSAGDADGFVARLNAAG
                     TALVYSSYVGGSRGDVINAVTIDTQGAIYLTGSTESEDFPTQGPMQIQMGGQRDAFVT
                     KLNPANPPQTQLVYSAFVGGSDMEVGLAIAVQSGNVYLAGSTRSSNFPTVIPLQPTYG
                     GSNGFYGDAFVVAINSSGTQVLSSTYLGGEGEDVATGIAVLNGRGDVIITGQTKSTSF
                     PLKSAFQSTIRGESDGFVARLFESRSLTYASYLGGDGNELYNGIASAGPDHYYITGQT
                     SSAVYPGADPVDPLERRSDLVVVHVGETGAGVLPGIRDVTRLGGSGNDASESIAVDGS
                     GNIYLGGRTRSADFPILGPVQDTFGGGTQDAFVMKYRPCTTLTLSPATVPQATFESSY
                     VQPFTASGGAEPYRFLITYGGLPAGMTLTEDGSLSGVPVQPGEFLFTLTAIDQTGCNT
                     SRTYNFTVTCGNFTVTPDSLPLGAVNNTYTQTLSVTGGVGPYTFGLKSGTVPPGIELS
                     PDGIVTGTPTVVGNYLFTIRITDSRGCFVDKVLNLVVAACPTFTFEPSGSAYLLAPPG
                     VPF"
BASE COUNT          389 a          520 c          441 g          358 t
ORIGIN      
        1 taaatggtac tcctggcggc gccagcaggt aggcactgcc tgatggttca aaggtaaacg
       61 tcggacaggc cgccaccacc agattcaata ccttatccac aaaacaccca cgtgaatcgg
      121 tgatccgaat cgtgaataga taattcccca ccaccgtcgg ggttccagtc actattccgt
      181 cagggctcag ttcaattccg ggtggcaccg tgcctgactt caacccaaat gtatatggcc
      241 cgacccctcc ggtcacactt aaggtctggg tataggtgtt attgacggcc ccaagcggca
      301 aagaatcggg tgtcaccgta aaattcccgc acgtcaccgt aaagttgtag gtccggctgg
      361 tattacatcc ggtctggtcg atggccgtga gggtaaacag aaattcccct ggctggactg
      421 gaacccctga caaactgcca tcttcagtca aggtcatccc ggcaggcagc ccgccatagg
      481 tgatcaaaaa gcgatagggc tccgctccgc cactggcggt aaacggctga acatatgagc
      541 tttcaaacgt cgcctggggc accgttgccg gagacagggt cagggtagtg caaggccggt
      601 atttcatgac aaacgcgtcc tgcgttccgc ctccaaaggt atcctgaacc ggtcccagga
      661 tcgggaaatc ggctgaccgg gttcgcccac ccagataaat attcccgctc ccatcgaccg
      721 cgatactttc actcgcatca tttccgcttc cacccaggcg ggtcacatcc cggattcctg
      781 gaaggacgcc ggcgccggtc tcgcccacgt gaaccaccac cagatctgat ctccgctcaa
      841 gcgggtcaac cggatcggcg ccagggtaca ccgcacttga tgtctgaccg gtaatgtaat
      901 aatggtccgg tccggcactg gcaattccat tgtacaattc attaccgtcg ccacccagat
      961 agctcgcata ggtgagtgac cgggattcaa acaaacgggc gacaaaccca tcggactcac
     1021 cccgaatcgt actctggaag gcacttttta atgggaacga agtggatttg gtttgaccgg
     1081 tgatgatcac atctccccga ccattgagga ccgcaatccc ggttgccaca tcctcccctt
     1141 ccccacccag ataggttgaa ctcagcacct gcgtcccgga actgttgatg gcgacgacaa
     1201 aggcatcccc ataaaaaccg ttgctgccgc cataggttgg ttgaagcggg atgactgtcg
     1261 gaaaattaga ggaccgggtg cttccggcca ggtagacatt gccgctttgg acggcaatcg
     1321 ccagacccac ctccatgtca cttccaccaa caaaggctga atacacgagt tgtgtctggg
     1381 gcgggttagc cgggttcagt ttggtcacaa acgcgtcgcg ctggcctccc atctggattt
     1441 gcatggggcc ttgtgtcggg aaatcctcag attcagtgct tccagtcaga tagatcgcgc
     1501 cttgggtgtc aatcgtgacg gcattgatga catccccccg actccctccg acgtatgaac
     1561 tgtagaccag cgcggttcct gccgcattca atctggcaac aaacccatcc gcgtctcccg
     1621 cactcacggc ctggcgggca ttgtgaaccg ggaagtcagt tgattcggta attccggcaa
     1681 ctgtcacctg gcccaaacta tccaccac
//
LOCUS       DAOQDO010001611         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75905_length_1707_cov_2.061138, whole genome shotgun sequence.
ACCESSION   DAOQDO010001611 DAOQDO010000000
VERSION     DAOQDO010001611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75905_length_1707_cov_2.061138"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PKZ53_21175"
     CDS             <1..1252
                     /locus_tag="PKZ53_21175"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43008.1"
                     /translation="TVLLEYSLGDQQSYLWVATQTSLVSYELPRKAEVGKAAQKVYEC
                     LIAQGLEAEFLQGATDPQAQARQKVMFNQYVAQKNAEFPQAAAELSQMIVQPAAALLS
                     RRKRVLIVADGWLQYIPFAALPDPGVPVRKAQPLIVNHEILNLPSASTLSVLQNNLTG
                     RQLAPKKMALLADPVFSPADDRVRAVTSQVSQPVAENLLACPWFSNRGFTLDRLPATR
                     KLAHIVRSLVPPGQYTIALDFGANLNFATSNDLSHFQIVHFATHGMLNSENPDWSGLI
                     LSLVDVTGHSVPGFLTTLETFTLNLPAELVVLQGCQTGFGLDPFKTETGKLQSQSITN
                     EGLTGLTRGLMYSGARRVLVTLWNVRDDSSSELMKRFYQKHLHSKNPMPPLAALRAAQ
                     LEMRHTPNWKSPYYWAGFLFYGQP"
     gene            complement(1264..>1707)
                     /locus_tag="PKZ53_21180"
     CDS             complement(1264..>1707)
                     /locus_tag="PKZ53_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43009.1"
                     /translation="PQSGTPQPVVPTPAPLPAPRKVPRKPALPKLQVPEPDRVMATPG
                     GVTEFEEIKTVWIQSVEPAGHSGKSIRQVLRETLEANGFSVRESPPADAKVSIRIRKK
                     AITPDRVALTLRFLGGQIWEAEAEAETLEGALEKALKTLVLVEKK"
BASE COUNT          401 a          450 c          431 g          425 t
ORIGIN      
        1 tacggtcctg ctggaatatt cacttggtga ccagcaaagc tatctgtggg tggctactca
       61 gacctcgctg gtcagctatg aacttcccag gaaagcggaa gttgggaaag ctgcccaaaa
      121 agtgtatgaa tgtttgattg cacaaggtct ggaggcagag tttctgcaag gggcgactga
      181 cccgcaagcc caagctcgtc agaaggtcat gtttaaccag tacgttgccc aaaaaaatgc
      241 tgaatttcca caagcggcag cagagttgag ccaaatgatt gttcaacctg ctgcagcttt
      301 gctctccagg cgcaaacggg tactcatcgt cgcagacggc tggctacagt atattccctt
      361 tgccgcattg ccagacccag gcgttccggt tcggaaggct cagccgctga ttgtcaatca
      421 cgaaattttg aacctaccct cagcttctac tttatcggtt ttgcaaaaca acttaactgg
      481 tcggcaatta gctcccaaaa aaatggcact gctggcagac ccggtgtttt cgccagcaga
      541 tgaccgtgtt cgcgccgtaa cgagccaggt cagccagcct gtggcagaaa atctactcgc
      601 ctgcccctgg ttctcaaatc gcggcttcac gctggatcgg ttgccggcta cgcgaaaact
      661 agcgcacatt gtcaggtcac ttgtaccacc tgggcaatac acaatcgcgc ttgactttgg
      721 tgcgaacctc aactttgcaa ccagcaatga tctcagtcac ttccaaattg ttcattttgc
      781 tactcatgga atgctcaatt ctgaaaaccc ggattggtct ggactgattt tgtcgctggt
      841 tgatgtaaca ggacattcgg taccaggatt tctgacgact ttagaaacgt ttacccttaa
      901 ccttcctgcc gaactggtgg tgctccaggg ctgccagacc ggctttgggc ttgatccctt
      961 caaaactgaa accggcaagc tccaatctca gagtataacc aacgaagggc ttaccggcct
     1021 gacccgtggc ttgatgtata gcggcgcccg gcgggtcctg gtgaccttat ggaatgtgcg
     1081 ggatgattct tcaagtgaac tgatgaagcg gttctaccaa aaacaccttc actccaaaaa
     1141 tccaatgccg ccactggcgg cattgcgagc cgcacagctt gaaatgcggc ataccccaaa
     1201 ctggaaatcg ccttattact gggctggatt tctcttctat ggccagccat agagaaattt
     1261 gccttatttt ttttcaacca gcaccagagt tttgagagct ttctcaagag ccccttccag
     1321 ggtttcggct tcagcctcgg cttcccagat ttgtccgcca aggaatcgca gggtcagagc
     1381 tacccgatca ggggtaattg cttttttcct gatacggatg cttacttttg catcagccgg
     1441 gggtgactca cggacagaaa acccattggc ctccagggtt tctctcagca cttgacggat
     1501 agattttcca gaatggccag ccggttcaac cgattgaatc caaacagttt taatttcttc
     1561 aaattctgtc acgccgccag gtgttgccat aacccgatca ggctctggaa cctgcaattt
     1621 gggaagagca ggtttgcgcg gaactttgcg gggagcggga aggggagcgg gtgtcggtac
     1681 caccggttgg ggcgtacctg attgagg
//
LOCUS       DAOQDO010001612         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75936_length_1706_cov_4.307692, whole genome shotgun sequence.
ACCESSION   DAOQDO010001612 DAOQDO010000000
VERSION     DAOQDO010001612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75936_length_1706_cov_4.307692"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1706
                     /locus_tag="PKZ53_21185"
     CDS             <1..>1706
                     /locus_tag="PKZ53_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43010.1"
                     /translation="QWNEPALADYALPTETWREVVARRQRYAALRARLETGAIHDINQ
                     LITENLNLHQFAVDVIEQCSIPGFLFAFWKALERLTILDPTCGAGDFLLAALKTLEPL
                     YSACFSRMQTFLARLPLPGQSTDFEYFSQFALIQKVAECFPNQPYFVLFSIVTRNLFG
                     VDILEDATEVAGQRLLFKLIAQVTDIADTARLADIRLNLRTGNALVGSVTPKAIPEID
                     QSDRFLKDIDHQNPMHWFDEFPEVFARGGFEVIVGNPPYVKLNKHTIDYQLSGFTTLP
                     CQDLYACVLERAYALLKNGGRLGVIVPISMFGTDGFATLQAETLRQFDCLWTAFFANR
                     PTQLFDGAQKRLTLVLGRKGTQHQTKIHTTAYLRWSKEERESLFETRIGYATPVSPFQ
                     VFAQALEKLGSELEMNLFSKLVSASSGRMRESLCQNGKERCYYTRKFGYFLAFTDFIP
                     HMTDLKTRQVRRPSELKELRFQSQDTCLAAIAVLSSATFFWFWNVLSDCRNLNRRDIL
                     AFPLNVASLPGDLKQSLSELGRGYLEALKATSTTMLKSGLVVETFEYKACKPILNEID
                     RL"
BASE COUNT          399 a          409 c          436 g          462 t
ORIGIN      
        1 acagtggaat gagccagccc ttgccgatta tgcgttgccg actgaaacct ggcgcgaggt
       61 cgttgcccgg cgtcagcgat atgctgcgct tcgagcccgg ctggaaactg gcgccatcca
      121 cgacatcaat caactcatta ctgaaaatct aaatcttcat cagtttgcag tggatgtgat
      181 tgagcaatgc tcaatacctg ggttcttatt tgctttttgg aaagcactgg agcgactgac
      241 gatccttgat cctacctgtg gggccgggga ttttttgctc gcggcgctca aaacgctgga
      301 gccgttgtat agtgcctgtt ttagccggat gcagactttt ctcgctcggc ttcctcttcc
      361 tgggcaatcc accgattttg aatatttcag ccagtttgcg ctgattcaaa aagtggcgga
      421 gtgttttccg aaccagccgt attttgttct tttttcaatt gtgaccagaa atcttttcgg
      481 tgtggatatt ctggaagacg ccaccgaagt cgctggtcag cgtctgttgt tcaagttgat
      541 agcccaggtg actgacattg ctgataccgc acgactggca gatatcaggt tgaatctccg
      601 aactggaaat gctctggttg gatcagttac gccgaaagca attcccgaaa ttgatcagtc
      661 ggatcgtttt ctcaaagaca ttgaccacca aaacccgatg cactggtttg atgagtttcc
      721 cgaagtcttt gcccgtggcg ggtttgaggt gattgtgggg aatccgccct acgtcaaact
      781 caacaagcac acaattgact atcagctctc tggatttaca accctcccgt gccaggattt
      841 gtatgcctgt gtgttggagc gagcctatgc gctcttgaaa aatggtgggc gactcggagt
      901 gattgttccg atttcaatgt ttggtacaga tgggtttgcc acacttcagg ctgaaactct
      961 gaggcagttt gattgtctgt ggaccgcatt ttttgccaat cgtccgacgc aactttttga
     1021 cggtgcgcaa aaacgactga cacttgtgct gggaagaaag ggaactcagc accagacaaa
     1081 aattcacacc acggcctacc ttcgctggtc gaaagaagag cgggaaagtt tgtttgaaac
     1141 ccggattggg tacgccacac ccgtcagccc gtttcaggtc tttgcacagg cacttgaaaa
     1201 actcggcagt gagttagaga tgaatctgtt ttccaagctg gtatcggctt catctggccg
     1261 gatgcgggaa agtttgtgcc agaatggtaa ggagcggtgc tactacacac ggaaatttgg
     1321 ctatttcctt gcttttactg acttcattcc acatatgacg gacctgaaaa ccagacaggt
     1381 caggcgacca tctgaattaa aagagcttcg ctttcaatca caggacacct gtctggcggc
     1441 gattgcggtt ttgagttcag caaccttttt ctggttttgg aatgtgcttt cagactgccg
     1501 aaacctgaat cggcgcgaca tcctggcctt tccattgaat gttgccagtc tgccaggtga
     1561 cctgaaacag tcattgagcg agctggggcg aggttatctg gaggcactca aggcaacgtc
     1621 aaccaccatg ctcaaaagcg gacttgtcgt cgaaaccttt gagtacaaag cctgtaagcc
     1681 aattctcaat gaaattgatc ggcttt
//
LOCUS       DAOQDO010001613         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_75953_length_1706_cov_3.256208, whole genome shotgun sequence.
ACCESSION   DAOQDO010001613 DAOQDO010000000
VERSION     DAOQDO010001613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75953_length_1706_cov_3.256208"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1644
                     /locus_tag="PKZ53_21190"
     CDS             88..1644
                     /locus_tag="PKZ53_21190"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ43011.1"
                     /translation="MRAIILATGYDPNLQGLHERHAAAMLPLVDRPFLQHIVETLVDA
                     GVTEFDFVLSHKPHEIEHLLGTGVRWGSTFRFHLVRNPHQPFKALRAISVYYRQDAQT
                     PVFLVHSQCLIQAGFSSLIDPTQTAERVLMYDQASPVQPDRQWTGWAVVSPDIIDQIP
                     STAQFNEVEALLRKKITTPDQVIQVEKALWVESCPDLFDANVQVLQKQFSGLLIQGRE
                     VEPGIWISRNVSLHPTAKLIAPVFIGENCRIEPGVALGPNVVIGKDCVLSEGSLIANS
                     LVLPGSFVGEALELNESIVDRNRLINYAVGVSVPVSDDFVLGKMAENPIANWAQSLCS
                     RWVALVLLIVTSPLIVLTMIFLKLFRSGKVIFRRQVIELPTTSDPILWENISLLSFTP
                     TLTATTTPRDLLLRVLPGLINIVAGDLHFVGVAPRSESEIEAMSDDWKALYLHCKGGL
                     ITEALVQYGENPIEDELYAAEAYYSARMGIWRDLYLIFWYLCKVFNPFGRRLEFEEDP
                     TLSLNGRSLR"
BASE COUNT          401 a          411 c          433 g          461 t
ORIGIN      
        1 gaccatcccc gccgtgttcc tggggaaagg agcctattag ggctgaggac gttgaaaaag
       61 agtcaggatt tgatttagga aacatatatg cgtgcaatta ttcttgctac cggatacgac
      121 ccaaatttac aaggtctcca cgaacgccac gccgcagcca tgctgccact cgtggatcgc
      181 cctttcctgc aacatatcgt cgaaacgctg gtggatgccg gggtaaccga atttgacttt
      241 gtcctcagcc ataaaccaca cgaaatcgag catttgcttg gaactggggt tcgatggggg
      301 agcacgttcc gctttcatct ggttcgcaac ccgcatcaac catttaaagc actgcgggcc
      361 atttccgttt attacaggca ggatgcgcaa acaccagtct ttctggttca ttcgcaatgt
      421 ttgattcagg ctggatttag ctcgctgatt gatccaactc aaacggcaga acgggttttg
      481 atgtatgacc aggcttcccc agttcagcct gaccgacaat ggaccgggtg ggctgtggtg
      541 tctcctgata tcattgacca gattccgtca acggctcagt tcaatgaggt tgaggcgctc
      601 ttacgcaaga aaatcaccac gccggaccag gtcatccagg ttgaaaaagc cctctgggtt
      661 gaatcgtgtc ctgatttatt tgacgccaat gttcaggtgt tgcaaaaaca attttccggg
      721 ttgctcatcc agggtcgcga agttgaacca ggaatttgga tttcgcgcaa tgtcagcctt
      781 catccaacgg caaaactgat agcgcctgtt tttatcggtg aaaattgccg gattgaaccc
      841 ggagtcgccc ttggacccaa cgtcgtcatc ggaaaggatt gtgttttgag cgaaggcagc
      901 ctgattgcca attctctggt gctgccgggc agttttgtgg gtgaagccct cgaactcaat
      961 gaatccattg tggatcgcaa ccgactgatc aattacgccg tgggggtttc agttccagtt
     1021 tcggatgatt ttgtccttgg aaaaatggct gaaaacccaa ttgccaactg ggcacaaagt
     1081 ttatgctcca ggtgggtggc cctggttttg ttgatagtta cctctccgct tatcgtgttg
     1141 accatgatct ttctcaagct gtttcggagt ggaaaagtca tctttcgtcg tcaggtgatt
     1201 gaacttccga caaccagtga tccaatcctg tgggagaaca tttcacttct cagctttacc
     1261 ccaaccttga ccgccaccac cacccccaga gatttgctcc tgcgcgttct gccgggcttg
     1321 atcaatattg tggccggaga tcttcatttt gtgggtgttg ccccccggtc agaatctgaa
     1381 attgaggcca tgtcagacga ttggaaagcc ctgtacctgc attgcaaagg tggcttgatc
     1441 accgaagccc tggtgcaata tggtgaaaat ccgattgaag acgaactcta tgccgctgag
     1501 gcctattatt cagctcgcat gggcatctgg cgcgacctgt accttatttt ttggtactta
     1561 tgcaaagtct ttaacccgtt tggccgtcgg ctggaatttg aagaagaccc tacgctgagt
     1621 ttaaatggtc gttcattgcg gtgattgatt gagaatgaag aatgaagaat gaagaataga
     1681 cattatcgga gttaggtgtt tacgaa
//
LOCUS       DAOQDO010001614         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76478_length_1700_cov_3.343465, whole genome shotgun sequence.
ACCESSION   DAOQDO010001614 DAOQDO010000000
VERSION     DAOQDO010001614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76478_length_1700_cov_3.343465"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1056
                     /gene="panC"
                     /locus_tag="PKZ53_21195"
     CDS             193..1056
                     /gene="panC"
                     /locus_tag="PKZ53_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNJ43012.1"
                     /translation="MEIINRIPRMRMLSRKLRRDNRKIGFVPTMGALHDGHLSLIRRA
                     QQMSDVVIVSIFINPIQFGPSEDFEVYPRDLARDAEQIANKGVDYLFAPSIDEMYPED
                     FMTYVVTENLSSRLCGASRPGHFRGVTTIVNKLFNIITPDFAFFGQKDAQQVIIIQRM
                     VRDLCMDTEVVVCPIIREVDGIAMSSRNQYLDPEERQAGLVLYRSIELARNMYLDGER
                     DCPKIVGAIRELIEKEPLARIDYVAAVDVDTLEPLNEIDDDTSVLIALAVFIGRTRLI
                     DNTVLKPEIEN"
     gene            1255..>1700
                     /locus_tag="PKZ53_21200"
     CDS             1255..>1700
                     /locus_tag="PKZ53_21200"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNJ43013.1"
                     /translation="MLHIQTAEHMTEVDRLTTETYGIKGLILMENASAGAGREIAQFF
                     GPVDGKSIRVFCGKGNNGGDGAALARQLWMRGAKVEVLLFGKREVTRGDARVNFDIIS
                     RLAETETKLIHFKEIISETDFKNLIQQNPHVPDLHIDALFGTGLKRG"
BASE COUNT          453 a          397 c          415 g          435 t
ORIGIN      
        1 tagtaatatc gctcttgcaa cctaaaacaa ttcgacttac tcttcagacc ctcataaatt
       61 ttatgagttg agaacaattt tatataaact caatgattgg tttcgatact ctacggacta
      121 ttgaccactg actgatatca acacgtcgtt gcccctgaca ctacctgtcc ttgccacagt
      181 gctggaaaaa tcatggaaat catcaatcga attccccgaa tgcggatgtt gtcgcgaaaa
      241 ctgcgtcgcg ataaccggaa aatcggattt gttcccacga tgggcgcact tcatgacggc
      301 catttgagcc tgattcggcg tgcccagcag atgtcggatg tcgtcatcgt ctcgattttt
      361 atcaatccaa ttcagtttgg ccccagtgaa gatttcgaag tctatccgcg cgacctggcc
      421 cgggatgctg agcaaattgc caataaaggg gtagattatc tgtttgctcc cagtattgat
      481 gaaatgtatc cggaagattt catgacctat gtggtgaccg aaaatctttc ctcacgcctc
      541 tgtggcgcca gccgtccagg gcattttcgg ggcgtcacca cgattgtgaa taaactgttt
      601 aatatcatca cgcctgattt tgcctttttt ggtcaaaaag acgcgcagca ggttatcatc
      661 attcaacgga tggtgcgcga tctgtgtatg gatactgagg tggtggtatg cccgattatt
      721 cgtgaagttg acggcatcgc catgtcgtct cgcaatcagt atcttgaccc ggaagaacgc
      781 caggccggac tggttctcta ccggtcaata gaactggccc ggaatatgta cctggatgga
      841 gagcgtgact gcccaaaaat tgtcggagcg attcgggaat tgattgaaaa agaaccactt
      901 gctcgaattg actacgttgc cgctgtggac gttgataccc ttgagccact caatgaaatt
      961 gatgatgaca cctcagtttt aattgctctg gctgtgttta ttggccggac ccgcctcatc
     1021 gacaacactg ttcttaaacc tgaaatcgaa aattagtgac gctctggtgg taagtcgtta
     1081 gacaggcaag tcaatgggaa aaatggtttt gaacccagga actgagccaa gaatcaaaat
     1141 cctttcaatt cttcattcgc agcagaaacc aatcgtttcg aagacgcacc agtcaccaag
     1201 caccaggcac caagcaccaa gcaccaagga ctaaaaacaa agaaccagga acctatgctg
     1261 catatccaga ctgccgagca catgacagaa gtagatcgtc tcacgaccga gacatatggg
     1321 atcaaaggac ttattttaat ggagaacgct tcagcgggcg cggggcgtga gattgcccag
     1381 ttttttggtc ccgtcgacgg aaaatcaatt cgagtgtttt gtggtaaagg gaataatggg
     1441 ggagatggcg ctgccctggc ccgtcagctc tggatgcgtg gcgccaaggt tgaagtcctg
     1501 ttgtttggaa aacgtgaagt aactcgtggg gatgcccggg tcaacttcga tattatttcc
     1561 cggctggccg aaacggagac caaattgatt catttcaagg aaatcatttc ggagacagat
     1621 ttcaagaatc tgatccagca aaacccgcat gttcctgatt tacacataga tgcgctgttt
     1681 gggaccggat tgaagcgcgg
//
LOCUS       DAOQDO010001615         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76523_length_1700_cov_2.165957, whole genome shotgun sequence.
ACCESSION   DAOQDO010001615 DAOQDO010000000
VERSION     DAOQDO010001615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76523_length_1700_cov_2.165957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1082)
                     /locus_tag="PKZ53_21205"
     CDS             complement(81..1082)
                     /locus_tag="PKZ53_21205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ43014.1"
                     /translation="MFYLMGVLLFCLGSLPVKAQTEKTQPDDVIRQHIDVVTFDAFVH
                     THKTGLPIANLKATDFKLYEDGVEKEIEYFSQDKLPLSVLFLLDVSGSVLPIYKELRD
                     EALRAVGRFKPEDEIALMVFSTEAQLVQGFTKDRDQFADALGSLEQSVKVGGGTFLNE
                     GMYHATRYLRNAATPSNRKVVIVITDNLASKPLKKGMTHNEKEAMAELIESGGVVCGI
                     IVKSWISNVATYHPVAVLMRTLTQPGSVNHYAEESGGTVIPSQTEQFEETLGKLVDNL
                     RARYSIGYSCSPDAQDGKLHKIKLKPRPEVEKREGKIAIKTIRGFKFSKTGERTRTK"
     gene            complement(1115..>1700)
                     /locus_tag="PKZ53_21210"
     CDS             complement(1115..>1700)
                     /locus_tag="PKZ53_21210"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ43015.1"
                     /translation="WVLGLAISLSLLNVNLAGVLAALGITGAALGLGLRDVISNMVCG
                     IILLSVQPFSLGDTVVIEGNEGTVEDIKIRVTTLRTGDGKQIQIPNSRVFAANITNLS
                     AYTQRRPSFLIKVAGFHQPDAVRKILLESVKNVPGVLAEPAASVQLTDMNGEALTFEI
                     SFSVDTTRASLSDVQRTVRETVRARLLANHIRLV"
BASE COUNT          430 a          451 c          381 g          438 t
ORIGIN      
        1 cgtgtgagaa aacagtcaag caattcaata aaacagactt agcgaactac tgactgctga
       61 ctactgacta cgaactggta ttattttgtc ctggtacgct cacctgtttt tgaaaatttg
      121 aagccacgaa tggttttgat ggcaattttt ccttcgcgct tctcgacttc cgggcgtggc
      181 ttcaatttaa ttttgtggag tttgccatct tgagcgtcag gtgagcagct atacccaatg
      241 ctgtaccgcg ctcgcaggtt atcaaccagc ttgccgagcg tctcctcaaa ctgctcggtt
      301 tgactgggaa taactgtccc tcctgattct tcggcatagt gattgaccga tccaggctgg
      361 gtcagggttc gcatcagaac agcaactggg tgataggtgg caacgttaga aatccagctt
      421 ttgacgatga tgccacacac caccccacct gattcgatca actcggccat tgcctccttc
      481 tcattgtggg tcataccttt tttcaacggt ttacttgcca gattatcagt gatcacaatc
      541 accacttttc gattactcgg agtggccgcg ttccgtaaat accgcgtggc gtgatacatc
      601 ccttcattga gaaacgtccc accgccaacc ttgacacttt gctcaaggct tcccagtgca
      661 tcggcaaact ggtctcgatc tttggtaaac ccttgaacaa gctgggcttc cgttgaaaaa
      721 accatcaggg caatttcatc ttcgggtttg aaacgcccaa ccgcgcggag ggcttcgtca
      781 cgcagttcct tgtaaattgg caaaacgctt ccgctgacat caagcaaaaa aagcaccgaa
      841 agcgggagtt tatcctgact gaagtactcg atttccttct caacaccatc ttcgtacaac
      901 ttaaaatccg tcgctttcaa gtttgcaatg ggaaggccgg ttttgtgcgt gtgaacaaac
      961 gcatcaaatg tcacaacatc aatgtgttga cgaatcacat cgtcaggttg agttttctcg
     1021 gtttgtgctt ttaccgggag gcttcccaga caaaacaaaa gcacacccat cagataaaac
     1081 atcttcatgc tgctcaatta cctttcaaat ggaattaaac caaccggata tggttggcta
     1141 gcagccgggc ccggacggtt tcccgcaccg tgcgctgaac gtcgctcagg gaagcgcggg
     1201 tggtatcaac tgaaaatgaa atctcaaagg tgagagcttc tccgttcata tccgttaact
     1261 gaaccgaggc ggctggctcg gctagtacgc cgggaacgtt tttgacagat tccaataaaa
     1321 tttttctgac ggcatctggt tggtggaaac cagcaacttt gatcagaaaa cttgggcgcc
     1381 gctgggtata agctgaaagg ttggtaatgt tggccgcaaa cacccggcta tttggaatct
     1441 ggatttgttt cccatcaccg gtgcgcagcg ttgtcacccg gattttgatg tcttcaaccg
     1501 tgccttcatt cccttcaatc accaccgtat caccaagact aaagggctgc acgctcaata
     1561 agataatccc gcagaccata ttggaaatca catctcgcaa ccccaaccct aatgctgccc
     1621 cggtgattcc caatgccgcc agcactccgg ccagattcac attaagcagg gaaagcgaaa
     1681 ttgccagccc aagaacccac
//
LOCUS       DAOQDO010001616         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76687_length_1698_cov_1.825320, whole genome shotgun sequence.
ACCESSION   DAOQDO010001616 DAOQDO010000000
VERSION     DAOQDO010001616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76687_length_1698_cov_1.825320"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKZ53_21215"
     CDS             complement(<1..230)
                     /locus_tag="PKZ53_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43016.1"
                     /translation="MLASWRSFEALAARVAVYQAHEHQINALYWQLVQIVALQAFELR
                     HALANMDCPGSEAKPQHSHSQSFRKSRSQSTSP"
     gene            886..1644
                     /locus_tag="PKZ53_21220"
     CDS             886..1644
                     /locus_tag="PKZ53_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNJ43017.1"
                     /translation="MSSQNSRRHFLKTTALTGAGMTLAGVPLLDKVVEADAVNLIYEA
                     TQLTDATGKFALPALPYEFKALEPVIDAKTVELHHDKHHKGYVDGANKAEEKLAAARA
                     DGNFDLVKHWSRELAFHGSGHILHTIYWTNLSGKGGGEPKGDLAKHIAKDFGDFAKFK
                     MHLSKATTAVEASGWGVLAYQVFSKNLTVLQIEKHQDLTTMGAIPLLVIDVWEHAYYL
                     TYQNKRGEYVDKIFNIINWDNIAQRLDAALKAGG"
BASE COUNT          469 a          406 c          407 g          416 t
ORIGIN      
        1 ggtgacgttg actggcttcg acttttccta aatgattgag aatgagagtg ttgtggtttt
       61 gcttctgaac cggggcaatc catatttgcc agggcatgcc gcaactcaaa agcctggagc
      121 gcgacgatct ggacaagttg ccagtagagc gcattgatct ggtgttcgtg ggcctggtac
      181 accgctaccc gggcggccag tgcttcaaaa ctgcgccatg atgccagcat tatagaattc
      241 caaccagtaa atagaatgaa cgggcgaagg gcccaaccat atcacaatgg aggacgtcgc
      301 tcctgaaaat tataccagct tgtagtcagt agatcgtagt cagtagtcca ctaactccat
      361 ttgatcgaat aatttgacta tttcctaata cgaggattcc attccaaaat ggtattacgg
      421 caatgatcag gccaaaaaga acagatttct atttgtttgg gtggggagag cgccggaaag
      481 tataaaccgg ttaacgcagg gtgagaactc gcgacctggt acaacttccg tctggaaaca
      541 attgggggag aaagtagttg gtgggattca gacattatgc aaagggacag cttttgcagg
      601 cttttccctt tttatatttt ttgcagcatt tctttttagc cataactcca catcaggtaa
      661 ccaggaagag gataaaaggt tttgaaaacc attttcaagt acgtgaatct taggcgtcag
      721 gttctggcct gtcaagcatc ccttgcttga attttcctga catggatccc gcgacggttt
      781 ttcaactgcc gctctggggt gatatggtag ggagcattcc tggctgaatc acatcgtgct
      841 caactctgat aatcaccctg tttttttcat ctggagaacc tgcctatgtc ttctcaaaat
      901 tcacgtcgtc attttctcaa aaccactgct ctgaccggcg ccggaatgac gctggctggc
      961 gttccgctcc tggacaaagt cgtcgaagct gatgccgtca atctgatcta tgaagccact
     1021 caactgaccg atgccaccgg aaaatttgca cttccggcgc ttccatatga atttaaagca
     1081 cttgaaccgg tcattgatgc caaaaccgtt gaactccacc acgacaaaca tcacaaaggc
     1141 tatgttgacg gcgccaacaa agccgaagaa aaactggcgg cggcccgcgc tgatgggaat
     1201 tttgatctgg tcaaacactg gtctcgcgaa ctggcgttcc acggttcagg ccatatcctg
     1261 cacaccattt actggacaaa cctctctggc aaaggcggag gcgaacccaa aggtgatctg
     1321 gccaaacaca ttgccaaaga ctttggcgat tttgccaaat tcaagatgca cctttccaaa
     1381 gccaccacag cagtcgaagc ctccggctgg ggtgtgctgg cgtatcaggt cttttccaaa
     1441 aacctgacgg tgctccaaat tgagaaacac caggatttaa ccacaatggg cgctattcca
     1501 ttgttagtga ttgacgtatg ggagcacgcc tattacctca cctaccagaa caaacgcggc
     1561 gaatacgttg ataaaatctt taacatcatc aactgggata acatcgccca gcgcctggat
     1621 gcagcactca aagccggcgg ctaaggaatg aagaatgaag aatgaagaat gaagaatgaa
     1681 gaatgaagaa tgaagaat
//
LOCUS       DAOQDO010001617         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76761_length_1697_cov_2.359318, whole genome shotgun sequence.
ACCESSION   DAOQDO010001617 DAOQDO010000000
VERSION     DAOQDO010001617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76761_length_1697_cov_2.359318"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..536)
                     /locus_tag="PKZ53_21225"
     CDS             complement(114..536)
                     /locus_tag="PKZ53_21225"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HNJ43018.1"
                     /translation="MTHKTWFSVKCIFRHADRGVGPRQNYEERVVLIRARKLSKAIKI
                     AKREAKKYARALSHCSLVGVVDCFGLFDKVGHKAEVFAVMHQSDLDPSEYLNHFYPPS
                     PQDCEAEGKTHRFYNQGKGYSACYHCQVVKLRKTTCGN"
     gene            complement(555..1511)
                     /locus_tag="PKZ53_21230"
     CDS             complement(555..1511)
                     /locus_tag="PKZ53_21230"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNJ43019.1"
                     /translation="MSKNSETLKPDITDIAQTIERYCPDWKIDSIHWMGEGDFCRAYM
                     VNAAWVFRFGKHQTARESLRRESCLLPHLASAVTLQIPAPQVFAFDESAGVGFAGYPL
                     LPGVALTQEKYLNLDDTDRTRCAGQVATFLNQLHGIELSIAVDCGVPIRNYATEYADL
                     LERAQFELFPRLAKTERLFIEQVINTYLASDDTLNYHPTLLHGDLSPDHVLFDDTSQT
                     VTGIIDFGDMMIGDPAWDLVFIYEDFGLDFLSRVLTGFHHTDRTALFNRLFQLYLLAA
                     IDWAAGSRARGEAEFEEALELIHELQREATQQREYLFLVCKE"
     gene            complement(1504..>1697)
                     /locus_tag="PKZ53_21235"
     CDS             complement(1504..>1697)
                     /locus_tag="PKZ53_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43020.1"
                     /translation="LAQSQKSGVNRESGQYLASLSLIVRPEGIFLQSGTFDREIPLVG
                     GGASGCWGLDFAVFNHPNE"
BASE COUNT          432 a          430 c          407 g          428 t
ORIGIN      
        1 gatttcaccc acggcgctta ccttcaccta ccatgcactg gcgattggat gtgttgtcca
       61 gaatccgatc tattggggga tgaccgagat tcccctggta attgcgtaat gcatcaattc
      121 ccgcaggttg tcttccttag ctttacaacc tggcagtgat aacaggctga ataccctttt
      181 ccttgattgt aaaagcggtg tgtttttccc tcagcctcac aatcttgtgg agatggggga
      241 taaaagtgat tcaggtactc agacgggtct aaatcgctct ggtgcataac ggcaaaaact
      301 tcagctttat gaccaacttt gtcaaatagt ccaaagcaat ctacaacgcc aaccagagag
      361 cagtgtgaca gggcacgagc gtattttttg gcttcccgtt tggcgatttt gatggctttt
      421 gacagtttgc gggcacgaat caacacaact cgctcttcgt aattttgtcg gggaccaact
      481 ccccggtctg cgtgtcggaa aatacatttc actgaaaacc aggttttgtg cgtcatactc
      541 aagccgttct tttcctattc tttacagacc aaaaacaaat attcccgttg ctgtgtggct
      601 tcacgttgca actcatggat tagctccaga gcttcttcaa attcagcttc accacgtgct
      661 cgcgagccag ccgcccagtc aattgcagca agcaaataca gttgaaacag gcgattgaac
      721 agggccgtcc ggtcagtgtg gtgaaagcct gtgagcactc gcgacagaaa atccaatccg
      781 aaatcttcat aaatgaaaac caggtcccag gccggatcgc caatcatcat gtctccgaaa
      841 tcaataatcc cagtgacggt ttgtgacgtg tcgtcaaaga gaacgtgatc agggctcaga
      901 tccccatgca gcaaggttgg gtgataattc aacgtgtcgt ccgatgccag ataggtgttg
      961 ataacctgtt cgataaaaag ccgttcggtt tttgccagcc tgggaaaaag ttcaaattgt
     1021 gcgcgctcca ggaggtcagc atattcagtg gcataatttc ggattggtac cccacaatcc
     1081 accgctatcg aaagctcaat cccgtggagt tgattgagaa acgtcgcaac ctgcccggca
     1141 catcgggttc ggtctgtatc atcgagattg aggtattttt cctgggtgag tgccacccct
     1201 ggcaaaagtg ggtacccggc aaaccccact ccagccgatt catcgaaggc aaaaacctgt
     1261 ggagctggaa tctggagcgt tacggcactg gccaaatgcg ggagcagaca ggattcacgt
     1321 cgcaggcttt cccgtgctgt ctgatgtttt ccaaagcgga atacccaggc ggcattgacc
     1381 atataggccc ggcaaaaatc gccctcaccc atccagtgaa tcgaatcaat cttccagtct
     1441 gggcaatagc gttcaatcgt ctgggcaatg tcggtgatat ctggtttcag ggtttcagag
     1501 tttttactca tttggatgat tgaacaccgc aaaatcaagc ccccaacatc cgctggcgcc
     1561 tccgccaacc aagggtattt ctcgatcaaa tgtaccagac tgaaggaaaa tcccttctgg
     1621 tcgaacaatc aaagaaagtg atgccaggta ctgacctgac tcgcggttga ctcctgattt
     1681 ttgagattga gccaggg
//
LOCUS       DAOQDO010001618         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76861_length_1696_cov_1.999391, whole genome shotgun sequence.
ACCESSION   DAOQDO010001618 DAOQDO010000000
VERSION     DAOQDO010001618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76861_length_1696_cov_1.999391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1696)
                     /locus_tag="PKZ53_21240"
     CDS             complement(<1..>1696)
                     /locus_tag="PKZ53_21240"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43021.1"
                     /translation="NEFAKAEALYQRALTIREKLTGSESEEVAESLSNLALVYYKIGD
                     YPRSEPLYQKSVAIKEKLFGPEHPLVAASLHNFGILYRAWGDYPKAETILKRALAMKE
                     RFFGKEHREVALTMNELALVYQETANYRQAELLQKWVLELNEKALGPDHPSVALALNN
                     LGLTYAQLGEYTQAEVLYRRALKIREKAFGPNHPGVAVILGNLGGLYHDRGDYVKAKE
                     LFYQALAIDEKVYGADHPNVLNTLNNLASASLNQKKYDEAEALYVRILAAREKRLGPD
                     HPDVAWALNSLAVTYDQQGFYDKSETFLHRALSIREKILKPDHPDIAQSYHRFGLLYA
                     KKGDLKQAEAYYQQALKIQEKSLGPTHPRMAETFRDLARIQELNRDFTQALTSKIREN
                     EIIELNWRQNLLSGSERQKLLYVRQTAQSFEWMINLQVQSLSQNPLAIREALLAALRR
                     KGRVLDAMALSIEHLRDRATPADQQLLDQLIAAKTQLSNLTLKGPGKDGVEIHRARLK
                     ELEDQIDQFEAQVSGRSFEFRAQFEPITIEAVQAALPKAAVLVEVLEDLFLHRLGHG"
BASE COUNT          388 a          462 c          396 g          450 t
ORIGIN      
        1 ccgtgtccaa ggcgatgcag gaaaaggtct tcaagaacct cgaccagtac cgcagctttc
       61 ggaagcgccg cctgaaccgc ctcgatagtg atcggctcaa actgggcgcg aaattcaaag
      121 ctccgcccac tgacttgggc ttcgaactgg tcaatctggt cttcaagttc cttgagcctg
      181 gcccgatgaa tctcaactcc atcttttcca ggtcctttta aagtcagatt ggacaattgt
      241 gttttggcgg caatcaattg atccagtaac tgctggtcag ccggagtggc acggtcccgc
      301 aggtgctcaa tactcaaagc catcgcatcc agaacccgcc ccttgcgcct gagcgctgcc
      361 agcaatgctt cccgaattgc cagcgggttc tgagaaagcg actggacctg caaattgatc
      421 atccactcaa aactttgggc tgtttgtctg acatagagta atttttggcg ctcagacccg
      481 cttaataaat tctgtctcca gttcaattca atgatttcat tttcgcggat tttagaggtc
      541 agtgcctgag tgaaatctcg attcagttcc tgaattctgg caagatctcg aaaggtctct
      601 gccatccgcg gatgggtcgg tcctaaactt ttctcctgaa ttttcagtgc ctgttgatag
      661 tatgcctccg cctgtttcaa atcgcctttt tttgcataca aaagcccaaa gcggtgatag
      721 ctttgggcaa tatcaggatg atcaggcttc aaaatctttt cccgaatact caaggcacgg
      781 tgcaagaacg tctcagattt gtcatagaac ccttgctgat catacgtcac agccagacta
      841 ttgagagccc aggccacatc cggatggtcg ggccccaatc gcttttcacg agccgccaga
      901 atgcgcacat acaacgcttc ggcctcatca tattttttct ggttcaagga tgctgacgcc
      961 agattgttga gggtattgag cacatttgga tggtcagcac catagacttt ttcgtcaatg
     1021 gccagcgcct ggtaaaataa ctccttcgcc ttaacgtaat ccccccggtc gtggtaaagc
     1081 cccccgagat tgcccagaat cacggccacg cctgggtgat tcggcccaaa tgctttttca
     1141 cgaattttta gggcccgccg gtacaaaact tccgcctggg tgtattcacc gagttgggca
     1201 taggttaagc cgaggttgtt gagcgccagc gccaccgaag gatgatctgg ccccagcgct
     1261 ttttcattca gttcaagaac ccatttttgc aaaagttccg cctggcgata gttcgccgtt
     1321 tcctggtaaa ccagagctaa ctcattcatg gtgagcgcca cttcgcgatg ttcttttccg
     1381 aaaaagcgct ctttcatggc cagcgcccgc ttcaaaatgg tttccgcttt cgggtaatcg
     1441 ccccaggccc gatacaaaat accgaaattg tgtagcgatg cggccaccag tggatgctct
     1501 ggaccaaaca atttttcctt gatggcaact gatttttggt acagcggctc tgatctgggg
     1561 tagtcgccga ttttgtaata gaccagcgcc agattcgata aactttcggc gacctcttct
     1621 gattcagacc cggtcagctt ttcccgaatc gtcaatgccc gctgatacaa ggcttcagcc
     1681 ttggcaaatt cattct
//
LOCUS       DAOQDO010001619         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76905_length_1695_cov_2.950610, whole genome shotgun sequence.
ACCESSION   DAOQDO010001619 DAOQDO010000000
VERSION     DAOQDO010001619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76905_length_1695_cov_2.950610"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="PKZ53_21245"
     CDS             complement(<1..1689)
                     /locus_tag="PKZ53_21245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43022.1"
                     /translation="MVKVAGTPEQVGGALVSANFFQTLGVGIQTGRLFSPEEAVGAGE
                     EPVILTQGLWKRCYGGQADIIGQTIDISGLKFTVVGVLPANFQFAPLEKAEAFALLRP
                     SPAQVERRFWFWVDVIGRMKPEVSASQIQAELGQFSHYLSQTFPESHRDIQVQAIPLQ
                     DYITGPVRPLLLALGGVVALILLLACTNIANLVLAQATTRSQEIAVRLALGASRWQML
                     RQLLIENLLLTLPGGCFGLALAHWSLDLLVHSLPQTILNKYPFFETVQIQPQILLLAL
                     GLTIATGVGFSLVPLISLNGFALQRVLRLGRHSGQNLTNMSLRQLLVVIEVASAFVLL
                     VGTGLLVQSLVSLLHTSPGFNTENLLTCRILLDGNQYREAHQRLTFFDRLQKQVAAIP
                     GLKGVSVVSQLPLTNGGDTGTPKVVGRPETESPRVEMFVRTISHNYFQVMEIPLATGR
                     WFSDHDQSTTKPVVVINQTLAARVFPNENPIGKQLAFSFLEGQTPPEIVGVVGDENVT
                     EFGKTMAPVVYFPLSQDALATARVIVRSTVSPDSLKQSLPQAIKNLDPTIPVSGV"
BASE COUNT          434 a          456 c          444 g          361 t
ORIGIN      
        1 cacaccagag acgggaatcg ttggatccag gtttttgatc gcttgcggga gactttgttt
       61 taatgaatct ggggaaacgg ttgatcgcac aatcactcta gccgtcgcca gggcatcctg
      121 ggaaagtgga aaatagacca ctggtgccat cgttttgcca aattcggtca cattttcatc
      181 acccaccaca ccgacaattt caggtggtgt ctgtccttcg aggaaagaaa aagcaagttg
      241 ttttccaatc gggttttcat tgggaaaaac tcgcgccgcc agtgtctggt tgatgacgac
      301 cactggtttg gtggtggact ggtcgtggtc tgaaaaccag cgacccgtgg caagtggaat
      361 ttccattacc tgaaaatagt tgtgagaaat ggttcgcaca aacatttcaa ccctggggga
      421 ttcagtctct gggcggccaa ccactttcgg agtccccgta tcgccaccgt tggtcagtgg
      481 caattgactc accactgaca cgcctttgag tccaggaatc gctgccacct gtttctgtaa
      541 ccgatcaaag aacgtcaggc gctggtgggc ttcacggtat tgatttccat cgagcaaaat
      601 ccgacaggtc agcaggttct cagtattgaa gccaggactg gtatgcaata aagacacgag
      661 gctttgaacc agcaaccccg ttccaaccag gagcacaaat gccgaggcga cttcgatcac
      721 caccagcagt tgccgcagtg acatgttcgt caggttttga cctgaatgac ggccaaggcg
      781 aaggacccgt tgcagtgcaa acccattgag cgatatcagg gggaccaaac taaaaccaac
      841 gcccgtggca atcgttagcc caagtgccag cagcaagatc tgaggttgga tttgaaccgt
      901 ttcgaaaaat ggatatttat tgagaatggt ttgtggcagc gaatgaacca gcaggtcgag
      961 gctccaatgg gccagcgcca ggccaaaaca tccaccaggc aaggtcaaaa gtagattttc
     1021 aatcagcaac tggcgaagca tttgccatcg gctggcaccc agcgccagtc gaaccgcaat
     1081 ttcctgtgac cgggtggtgg cctgagccag aaccagattg gcaatattgg tgcaggccag
     1141 caacaaaatc agcgccacca ccccacccag ggccagcaac aacggacgca cgggcccggt
     1201 gatgtaatcc tggagcggga tcgcctgaac ctgaatatcg cggtggcttt cggggaaagt
     1261 ttgggacagg taatgactga actggcccaa ctcagcctgg atttgggacg cggacacctc
     1321 tggtttcatc cgcccaatga catcaaccca aaaccagaac cgccgctcaa cctgggccgg
     1381 tgacgggcgc agcagagcaa aggcttcagc tttttcaagc ggggcaaatt gaaaattggc
     1441 tggtaacacg ccgactacgg tgaattttaa cccgctgata tcaatcgttt gcccgatgat
     1501 gtcagcctgt ccaccataac accgtttcca aagcccctgg gtgaggatca ctggctcttc
     1561 accggcacca accgcttctt caggtgagaa caaccgacct gtctgaatgc caacgcccag
     1621 tgtctggaaa aaattggctg aaaccagcgc cccgccaact tgttcaggtg tgcctgcaac
     1681 tttgaccacc gcatt
//
LOCUS       DAOQDO010001620         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76910_length_1695_cov_2.754878, whole genome shotgun sequence.
ACCESSION   DAOQDO010001620 DAOQDO010000000
VERSION     DAOQDO010001620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76910_length_1695_cov_2.754878"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="gcvPB"
                     /locus_tag="PKZ53_21250"
     CDS             complement(<1..1091)
                     /gene="gcvPB"
                     /locus_tag="PKZ53_21250"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNJ43023.1"
                     /translation="MADQATRQRIKKVRTHISQNEGLLFEISRSGSRAYVLPPSDVPE
                     PPIDQLISSNFLRQDDLEGMPELTEPEVVRHYTRLSTWNYGTDTGMFPLGSCTMKYNP
                     KINEWAARLPGFAITHPLAPEALVKGNLQLIKKLEDELCEITGMDTISLQPAAGAHGE
                     LTGIMMVRAALEARGNRRKRVLIPDSAHGTNPASAVMCGYSVETIASNARGLTDLEVL
                     EKKMDDSVAAIMLTNPNTLGLFEENIEKIAQLVHSRGGFVYMDGANMNAMCGVTRPGD
                     MGVDVMHLNLHKTFSTPHGGGGPGCGPVLVVKELEPFLPFPTLRRTEAGEVEFDWEQP
                     QSIGKVKAFWGNFGMMVRALAYIRALGPDG"
     gene            complement(1239..>1695)
                     /locus_tag="PKZ53_21255"
     CDS             complement(1239..>1695)
                     /locus_tag="PKZ53_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43024.1"
                     /translation="KIPPQLLTATQVRLLDATFLFGRLLVTGQIPEITEYRVNDLTFG
                     HTLNNSEFVVTDALYQQFLKFVRENSKETGGITTAMVTENEDYVRQQLRREVVTAAYG
                     SEVADEIVIAADTQMVRAIEELPNARLLAEKSRKVWLESSQRTPRRFGQ"
BASE COUNT          406 a          454 c          411 g          424 t
ORIGIN      
        1 ccatctggtc ccagcgcccg gatatacgcc agagcccgca ccatcatgcc aaaattgccc
       61 caaaacgctt ttactttgcc aattgattga ggttgctccc aatcaaattc gacctccccg
      121 gcttctgttc gtcgaagtgt tgggaagggt aaaaatggct ctaattcctt gaccaccagc
      181 accggtccac atccagggcc accaccaccg tgcggtgtcg agaaggtttt gtgcaggttg
      241 aggtgcatca cgtcaacccc catatcacca ggtcgagtca cgccgcacat ggcattcatg
      301 ttggcgccgt ccatatagac aaagccacct cgggaatgaa ccaactgggc aatcttctcg
      361 atattttctt caaataaccc cagcgtattc gggttggtca acataatggc cgcaaccgaa
      421 tcgtccatct tcttttctaa aacctcgagg tcggttaacc cacgagcatt ggaagcaatt
      481 gtttcaacac tgtaaccaca catcaccgca ctcgccggat tggtgccgtg ggctgagtct
      541 ggaatcaaaa cccgttttcg gcggttgcct cgggcttcga gcgcggcacg gaccatcata
      601 attccggtga gttcaccatg ggcgccggct gcaggttgaa gcgaaatggt gtccatcccg
      661 gtaatttcgc agagttcatc ttctaacttc ttgattaact gaagattccc tttcaccagt
      721 gcttctggcg ccagcggatg ggtaatcgca aaccctggta atcgagctgc ccattcattg
      781 attttggggt tgtacttcat cgtacaggac ccaagcggga acatgccggt gtctgttcca
      841 taattccagg ttgacagccg tgtgtagtga cgaaccacct cgggctcggt caattccggc
      901 atcccttcca aatcatcctg gcgcagaaaa ttggaagaaa tcaactggtc aattgggggt
      961 tcgggtacat ccgagggagg taacacatac gcccgactcc cagaccgtga tatttcaaac
     1021 aacaaacctt cattttgact gatatgggtt cttacttttt tgattcgttg acgcgttgcc
     1081 tgatctgcca tgccatacct cttaagagtg agttgtgagt agcaaagtag taaagctgat
     1141 attggaacta aaggacgtgt cagtaccacc tcctgaccgg gtggggttgg aagcaaggcc
     1201 cacccgctgc cgcaggtggt actgacttca ctctgttgct attgcccaaa ccgacggggg
     1261 gtacgttgac tgctttcaag ccaaactttg cgtgattttt cagccagcaa ccgtgcattc
     1321 gggagttctt caatcgctcg aaccatctga gtgtcagcgg caatcacgat ttcatcagcc
     1381 acttcactgc cataagcggc agtaacgact tcacgacgca actgctggcg aacatagtct
     1441 tcgttttcgg tcaccatggc agtggtaatg cctccggttt ccttcgaatt ctcgcggaca
     1501 aattttagaa attgctgata gagcgcatcg gtcacgacaa actcagaatt gtttaaagta
     1561 tgtccaaagg tcaaatcatt gacccgatat tcagtaatct ctggaatctg gcccgttacc
     1621 aggagccgac caaaaaggaa ggttgcatcc agcaatcgga cctgagtggc ggtcagaagc
     1681 tgcggtggga ttttt
//
LOCUS       DAOQDO010001621         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_76915_length_1695_cov_2.636585, whole genome shotgun sequence.
ACCESSION   DAOQDO010001621 DAOQDO010000000
VERSION     DAOQDO010001621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76915_length_1695_cov_2.636585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..747)
                     /locus_tag="PKZ53_21260"
     CDS             complement(58..747)
                     /locus_tag="PKZ53_21260"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HNJ43025.1"
                     /translation="MTRHEGEQYVKGFFEQAKPRLIRLTGRFVEDPQTIVSRAETIFD
                     EMLPELAYVEKPFHPLASALFVCTVNLAAYLALKERGVAVHDFGSAMLQGLARAPIPP
                     PTEPETEAERHQRYVQFLAAAETSQQNPAPGEDVFEFVPGNGTDFDFGHNIKSCAIYT
                     QCSKYDAQDVVPYMCGADDVMSDKGNQGLRRTGSIALGASQCDFRFKKGGEPLRLAEQ
                     YPDKIRISTRD"
     gene            complement(874..>1695)
                     /locus_tag="PKZ53_21265"
     CDS             complement(874..>1695)
                     /locus_tag="PKZ53_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43026.1"
                     /translation="RTFPTVLADRTANDGAHTIVVPQLTTQAGRIKVEAVGNVFFDIS
                     NTNFEVKPGTSTDTQPPTVTLLAPNGGESFTIGSGVPVRWTSTDNTGVASHSLELSLD
                     GGGTFPISAAASVDGSAQAFTFTATDQFASMRARVRITAQDAAGNKGLDASDADFAIV
                     QAGPNDTEPPQVTVTSPTGGKVKVSASKSFTATWQSRDNVGVTSQDVGLSIDSGTTFP
                     TTLATGLAGTVQSLELNSALIGKQKSKTARLRITARDAAGNSGTGISPVFTLVSK"
BASE COUNT          358 a          459 c          458 g          420 t
ORIGIN      
        1 gccctgcggg cgggagttgc caggttcaac ctatcttaca acacctttta ttcaagtcta
       61 atctctcgta ctaatccgaa tcttgtctgg gtactgttcg gccagtcgga gcggctcgcc
      121 gccctttttg aaccgaaaat cacactggct ggcaccaagg gcaatcgaac cggttctccg
      181 aaggccctgg ttgcctttgt cgctcatcac gtcatctgcc ccgcacatgt aaggcactac
      241 atcctgagca tcatactttg agcattgggt gtaaatcgcg caggatttga tattgtggcc
      301 aaagtcaaaa tccgttccgt ttccaggcac aaattcaaac acatcttcgc ctggggccgg
      361 gttttgttgc gaagtttcag ccgccgccag gaattgaaca tagcgttggt ggcgttcggc
      421 ttcggtttcg ggttcggttg ggggcggaat tggtgctcgg gccagaccct ggagcatggc
      481 gctcccaaaa tcatgcacag caacgccgcg ttctttcaaa gctaaataag cggcgagatt
      541 aaccgtgcag acaaacagcg ctgacgccag tggatgaaat ggtttctcaa cgtaggccag
      601 ctccggaagc atctcgtcaa agatcgtttc cgccctggaa acaatcgtct gtgggtcctc
      661 gacaaatcgc ccggtcagtc ggatcagccg gggtttggcc tgttcgaaga aacccttcac
      721 atattgttcg ccttcgtgtc gtgtcatgat tttttccttc aggtgctttg tgactggtgt
      781 tgcaatgaat ttcttcgaaa accctaaacc ctgaaccctg aaccctgaac cctgaatcct
      841 gaaccctgaa ccctgaatcc tgaacccggc ttcctacttc gaaaccaagg taaacaccgg
      901 acttatgccc gttccactgt ttccggcggc gtcccgggct gtaattcgga ggcgagccgt
      961 tttgctcttt tgttttccaa tcagtgccga attcaattcc aatgattgaa ctgtccccgc
     1021 gagcccggtt gccagtgttg tcgggaaggt cgtcccgcta tcaattgaca gtccgacatc
     1081 ctgtgacgtg acaccgacat tgtcacgtga ttgccacgtg gcggtaaatg acttgctggc
     1141 gctcactttg accttgccac cagttggact ggtcaccgtg acctgcggtg gttcggtatc
     1201 atttggcccg gcctggacaa tcgcaaaatc agcatctgac gcatcaagtc ctttatttcc
     1261 agcggcatcc tgggccgtga ttcgcactcg tgcccgcatg gaggcaaatt gatcggtggc
     1321 ggtaaaggta aatgcctggg ctgaaccatc aacggaagcc gccgccgaaa tggggaaggt
     1381 gccgccacca tccagcgaaa gttcaagcga atgtgacgcc acgccagtat tgtccgtcga
     1441 agtccagcgt actggaacgc ctgatccaat tgtgaatgat tctccaccgt ttggcgccag
     1501 cagggtaacg gttggcggtt gggtgtcagt tgatgtgccg ggtttgacct caaagttggt
     1561 gttggaaata tcaaagaaga cattgccgac tgcttcgact ttgatgcggc cagcctgggt
     1621 ggtcagttgg gggacaacaa tcgtgtgggc gccgtcattg gcggttcggt cagccagcac
     1681 ggttggaaag gttcg
//
LOCUS       DAOQDO010001622         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77062_length_1693_cov_4.677656, whole genome shotgun sequence.
ACCESSION   DAOQDO010001622 DAOQDO010000000
VERSION     DAOQDO010001622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77062_length_1693_cov_4.677656"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PKZ53_21270"
     CDS             complement(<1..1135)
                     /locus_tag="PKZ53_21270"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNJ43027.1"
                     /translation="MRRRILRLCCVLFFAGLWGLSLTNWPQSSGTQSLFTLVASAATS
                     EKGENLSSEYELPYGENLFAPSNAATVSGGFIKQEEFIPAARCAGCHTEAHAQWSESA
                     HRNSFREPFYQTNVKHLIRDRKIAVTRHCESCHNPAALFSGALSEKAIFARPFDEEGV
                     TCSVCHSIVSTNTTGIGSYVIDKPALLETADGTRLADVTDREILEDVPSHRRAMMRPL
                     LKTPEFCAACHKAAVVPELNQRKWLRSFAIYDEWQQSAFSNETVQPLARRDRQSCQDC
                     HMPEANGLRSHRWPGANTALPAFYNAPNQLAETQKLLQSGILSVDIFAIPTASTPNPE
                     PQTLNPAFQTLNPEPRTLAFDVVVANRNVGHAFPAELRDIFEAW"
BASE COUNT          424 a          393 c          459 g          417 t
ORIGIN      
        1 gccaggcttc aaaaatgtcg cggagttcgg ctgggaacgc gtggccgacg tttcggttgg
       61 cgacgacaac atcgaaagcc agggttcggg gttcagggtt cagggtttga aaagcaggat
      121 tcagggtttg gggttcagga tttggagttg aagcagttgg tatggcaaag atatcaaccg
      181 acaagattcc tgattgcaac aatttctggg tttcggcaag ctggtttgga gcattataaa
      241 acgctgggag ggccgtattg gcgcctggcc agcgatgtga ccggagaccg ttggcttccg
      301 gcatgtggca atcctggcag ctttggcggt cacgacgggc cagcggttga accgtttcat
      361 ttgaaaaagc cgattgctgc cattcgtcat aaatcgcgaa tgaacgcagc cacttccgct
      421 ggttgagttc aggaacgacc gccgctttgt gacaggcggc gcaaaactca ggtgttttca
      481 ggagcgggcg catcattgcc cggcggtgcg aaggcacgtc ctccaagatt tcacggtcgg
      541 tgacgtcagc cagccgtgtg ccatcagcgg tttcaagcag cgctggtttg tcaatcacgt
      601 agctcccgat gccggtggtg ttcgtcgaga caatgctgtg gcagaccgaa caggtcaccc
      661 cttcttcgtc aaatggacgg gcaaagattg ctttctcgga cagtgctccg gaaaacagag
      721 ctgccgggtt gtggcagctt tcacagtggc gggtgacggc aatttttcga tctcggatca
      781 ggtgcttgac gttggtctgg taaaacggtt cgcggaagga attgcgatgc gccgactcgc
      841 tccactgggc gtgggcttcg gtgtggcaac cggcacagcg ggcggctggg atgaattctt
      901 cctgtttgat aaatccaccg gagaccgtcg cggcattgga cggagcgaac aggttttcac
      961 cgtatggaag ttcatattca gaactcaaat tctcgccttt ctcgctggtt gccgcactgg
     1021 cgaccagggt gaaaagcgat tgagttcctg aagattgagg ccagttcgtc aggctcaatc
     1081 cccaaagacc ggcaaaaaag agcacacagc ataatcggag aatccgtcgg cgcatgggag
     1141 tcacagtcac tttgaaaaat aatcagtttg aactttgaga agtgtgtcgg gtattgatgt
     1201 tatttatcaa tttgcgtgcc acactggaat caacttcaat gaaagtaatc gttctcaaca
     1261 caacttattc tcaatacaat acttgctgtc gtatgtaccc gcattccggt ttggctgaat
     1321 cgctcaaaaa ccggcctggt tttagccttt tggttaaaac ttttcacctt ttggttaaaa
     1381 ctatctttgt tcaagcttcc aatagagtgt tgaaaagcat ttgacaccag atgaggtaca
     1441 aatgttccag ctctgattca aaattcagct aagtaactat ctatcaatca gatagatggc
     1501 ataatcccct gaaagaatca aaaccagagc acacaacccc tgagtactca cggtaagtac
     1561 aaagacgatg aagttttaca aagggccacg atgacctgac gacaaaggtc atcagcgaat
     1621 acagacaaat tcacctgcac aaacaggttt tttgccagca atgaataaag ttgtgagcgt
     1681 tgtctttcac ctt
//
LOCUS       DAOQDO010001623         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77072_length_1693_cov_3.256410, whole genome shotgun sequence.
ACCESSION   DAOQDO010001623 DAOQDO010000000
VERSION     DAOQDO010001623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77072_length_1693_cov_3.256410"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKZ53_21275"
     CDS             <1..184
                     /locus_tag="PKZ53_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43028.1"
                     /translation="VPVQLDTVGRYESPLAQQSFYLAVQARHRGDHQAAKALKAQARA
                     QERAFKVATRLRFNAG"
     gene            complement(192..1304)
                     /locus_tag="PKZ53_21280"
     CDS             complement(192..1304)
                     /locus_tag="PKZ53_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNJ43029.1"
                     /translation="MASLLETFATVPDPRKALNRRHKLIDILTIAICTVICGGTGYED
                     MELYGTSQQEWFQTFLELPAGIPSHDTFRDVFRRLDPKQFEEHFRHWVQGIAEVAGGT
                     VINLDGKTLRGSRRGVAPGIHLVSAWASQNQLVLGQLKTADHSNEITAIPELLRVLAL
                     QGCIVTIDAMGCQTAIARQITEQEGDYVLALKDNHPTLRTAVETMFDQARATKFTHLP
                     HTFHQTVDADHGRIETRRCWATSHLEPLPAALRDAWPGLTSLIRLDSIRDHQGTVSTE
                     SRYYLSSLPAHAEQLLSVIRSHWGIENRLHWVLDVTFREDDCRIRTGHGPENLTVLRH
                     FALNLLKQETSAKKSVRAKRLKAGWNHHYLLKVLTS"
     gene            complement(1400..>1693)
                     /locus_tag="PKZ53_21285"
     CDS             complement(1400..>1693)
                     /locus_tag="PKZ53_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43030.1"
                     /translation="LKDNRQKEIVPPGDEQTPWQQDSGDFEKHVAHAGTHRADQKPER
                     HPAHREGNGKEQGERKQGHKAVDGPRNDVLTPQADRVGRRLAQLFGNRVDTPG"
BASE COUNT          298 a          502 c          521 g          372 t
ORIGIN      
        1 cgttcccgtt cagctcgaca ccgtgggccg ctatgagtcg cccctcgccc aacagtcttt
       61 ctatctggcc gtccaggccc gccatcgggg cgaccatcag gctgccaaag ccctcaaggc
      121 tcaggcccgc gcccaggaac gcgccttcaa ggttgccacc cggctccgct tcaacgcagg
      181 gtaaacggga attaacttgt taatactttt aggagataat ggtgattcca gcccgctttc
      241 agccgtttgg cccggaccga tttcttggcc gatgtttcct gcttgagaag gttgagggcg
      301 aagtgtcgca ggaccgtcag gttttccggg ccatgaccag tccgaatgcg acaatcatct
      361 tcgcggaagg tgacatcgag gacccagtgg agccgatttt cgatccccca gtgggagcga
      421 atgacggaaa gcaactgttc ggcatgggcg ggcagactgg aaagatagta acgggactcg
      481 gtggagacgg tgccctggtg gtcccggatg gaatcgagcc ggatgaggga agtcagcccc
      541 ggccaggcgt cgcgcaaggc ggcgggcaat ggttcgaggt ggctggtcgc ccagcagcgg
      601 cgcgtttcaa tccgcccatg gtcggcgtcc acggtctggt gaaaggtgtg cggcaggtgg
      661 gtgaatttcg tggcccgggc ctggtcaaac atggtttcca cggcggtccg caaggtcggg
      721 tgattgtctt tcaaggcgag gacatagtca ccttcctgtt ctgtgatctg ccgggcaatg
      781 gcggtttggc agcccatggc gtcaatcgtg acgatgcagc cctggagcgc caggacccgg
      841 agcagttccg ggatggccgt gatttcattg gaatggtccg ccgttttgag ctggccgagc
      901 accagttggt tctgactggc ccaggcgctc acgaggtgaa ttccgggggc gacaccccgc
      961 cgcgacccac gcagggtttt gccgtcgagg ttgatcaccg ttccgccggc cacttccgcg
     1021 atgccctgca cccagtgccg gaagtgttcc tcaaattgct tggggtccag ccgccggaac
     1081 acgtcccgaa acgtgtcatg cgacgggatg cccgccggca attcgagaaa cgtttgaaac
     1141 cattcctgct ggctggtgcc atataactcc atatcttcgt acccggtccc gccgcagatc
     1201 accgtgcaga tggcaatcgt caggatatca atcaatttgt gccgccgatt cagtgctttc
     1261 cggggatcgg ggaccgtcgc aaaggtttcc agcaaactgg ccatggctgc ctccaagggt
     1321 gaaaaatggg gaaaacgcct ttctaccaga ttttgcccgg ccagccgatc cctttgatgc
     1381 ggaaatattt agttcccgtt taccctgggg tatcaacccg gttcccaaaa agttgagcca
     1441 gccgtcgccc aacccggtcg gcctgcggag tgagcacgtc attccgtggg ccgtctacag
     1501 ctttgtgtcc ctgcttgcgc tccccctgct ctttcccatt cccctcacgg tgggccgggt
     1561 gccgctctgg tttctggtct gcccggtggg tcccggcgtg agctacgtgt ttctcaaaat
     1621 cgccggagtc ctgctgccac ggggtttgct cgtcgcctgg tgggacaatc tctttttgcc
     1681 ggttgtcttt gag
//
LOCUS       DAOQDO010001624         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77140_length_1692_cov_3.237019, whole genome shotgun sequence.
ACCESSION   DAOQDO010001624 DAOQDO010000000
VERSION     DAOQDO010001624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77140_length_1692_cov_3.237019"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PKZ53_21290"
     CDS             complement(<1..1619)
                     /locus_tag="PKZ53_21290"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNJ43031.1"
                     /translation="MSDNEVGFEVPAYDPAYPLIIDPVVSVESSTYVGGSGNDSFSGV
                     VTDTNRNLYLCGFSASANFPVQSAFQSDLKGPTDVTVLKLDPAGELVYATYLGGQGGD
                     TSGDLTVDDEGNVYLTGVTSSTDFPVVNPAQRFSGGGNDVFVAKLNPEGTELVFSTYL
                     GGPVNGTSNQEQGTSIAVDQSGAVYVTGITSSTGFPTVNPFQSKLGGSSNFSDAFVTK
                     LSSNGSQIVFSTYLGGRGNTSENGLGIAPDSAGNVYVYGLTFNFRLSSLVIDFPVTSN
                     AFQREFGGGGSDLFVSKLNPSGNQLLYASYLGGSEDENATCTVGSCSNLLTKKQVAVD
                     EAGNFVIAGFTTSPNYPVRNPIQGTFGGSVDAVVTQINPDGSEIVFSTFLGGSGSDFG
                     TGVTVGSTGDLYVTGVTFSTNFPTVNPTQAAFAGPADVFVTQMNRNQGKLVFSTYLGG
                     KENDQPTEIFVDQADKIHVVGFTTSPDFPVQNAIQDKFSGGQDAFLTTFAEVEATPDF
                     TLTASPATQILTAGETKSVEVKLSGVPESAAP"
BASE COUNT          460 a          449 c          381 g          402 t
ORIGIN      
        1 ggtgccgcgc tctcgggcac gcccgacaac ttgacttcga ccgatttggt ctcaccagca
       61 gtcaaaatct gcgtagccgg actggctgtc aatgtaaaat ctggcgttgc ttccacttct
      121 gcaaaagtag tcaaaaaggc atcttgcccg cccgagaact tatcctgaat agcgttttgg
      181 accgggaaat ctggtgacgt cgtaaaccca accacatgaa ttttatccgc ctgatcaaca
      241 aaaatttcag tcggttgatc gttctctttt ccgccaagat aagttgaaaa gaccagtttt
      301 ccttgatttc ggttcatttg agtcacaaac acatcggctg gccccgcaaa agcggcctgg
      361 gttgggttga cagttggaaa attggtggaa aaagtcacgc cggttacata gaggtcgcca
      421 gttgaaccaa cggtaacccc cgttccaaaa tcgcttccac tgccgcccag aaaggttgaa
      481 aagacaattt ctgacccatc tggattaatt tgagtgacaa cagcatccac actgccacca
      541 aaggtgccct gaatcggatt ccgaaccgga taatttggtg aggtggtaaa accagcaatc
      601 acaaagttgc cagcctcatc tactgcaacc tgctttttgg tcaggagatt gctgcaggaa
      661 ccaaccgtac aggtggcatt ctcatcctca gacccaccga gataggatgc atacagaagc
      721 tgattgcccg aagggttcaa tttcgagaca aacagatcgc tgccaccacc accaaattca
      781 cgctggaagg cattggatgt cactggaaaa tcaatgacca gcgatgacaa cctgaaattg
      841 aacgtcaggc catacacata tacatttcca gcactatctg gagcaatgcc aagcccattt
      901 tcagaggtat ttcctcgtcc gcccagatag gttgaaaaca caatctgact tccgttgctg
      961 ctcagtttgg tcacaaaagc atctgaaaaa ttgctactcc cacccagttt tgattgaaag
     1021 ggattgactg ttggaaatcc ggttgagctt gtaattccag tcacatacac ggcccctgac
     1081 tggtcaaccg caatcgaggt cccctgttcc tggtttgagg taccattcac cggaccaccc
     1141 aggtaggttg aaaacaccag ttcggtccct tctggattta atttggcaac aaacacatca
     1201 ttgccgccgc ccgaaaaacg ctgggcgggg ttgaccaccg ggaaatcagt cgaactggtt
     1261 acgcccgtta aatagacatt gccctcatca tctaccgtta aatcaccaga ggtatcacca
     1321 ccctgtccac ccagataggt ggcatagacc agctcacccg ccggatcaag ttttaaaacc
     1381 gttacatcgg tgggaccttt caagtcggat tggaaggctg attgaaccgg gaaattggca
     1441 gaggctgaaa acccacacag gtacaggttg cggtttgtat ccgttacaac tcccgaaaaa
     1501 ctatcattcc cgcttccgcc aacatatgtt gacgattcga ccgaaaccac tggatcaata
     1561 atgagtggat aggccggatc ataggctggg acttcgaatc caacctcgtt gtctgacaca
     1621 atggaaaacc gcccttctac aactttccgt tcaccattga tctcctggta tagcgtcggg
     1681 tgtttttgag aa
//
LOCUS       DAOQDO010001625         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77168_length_1692_cov_2.570556, whole genome shotgun sequence.
ACCESSION   DAOQDO010001625 DAOQDO010000000
VERSION     DAOQDO010001625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77168_length_1692_cov_2.570556"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="PKZ53_21295"
     CDS             complement(<1..1536)
                     /locus_tag="PKZ53_21295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ43032.1"
                     /translation="MKRKLIALMIFVVWLMLQPVGINAQQNPDALVTQAEQAYAKKDY
                     ARSAELFVAATKLQADDSGLLYNAACACALAGRKDDAFDLLNRALKLGYGNSAQLKQD
                     TDFQSLREDPRWQITLDACTRNEKRLTLMWNNPALQSPYRENLSEDEKIAGLSKLWAE
                     VKFNFANFDLVPELDWDKLYLAYLPKVRQTKSTDEYYRVLAELVAQLKDGHTNLSPPN
                     ELALQWYAVPALSTRMIEDRVMVRRVWDSKLTEAGIKPGLEVVEINGMPVKAYAEKFI
                     QPYVAASTPQDAETRLFSYELLRGPVQQALELTFRTESGETFKKSVPRLNSEQWAALK
                     LPPSPILEFKVLESNVGLLVLNSFNDGKIEAEFAKVLPQLMTTDALIIDVRNNGGGNS
                     GYGWNILAHLTNKPFQTSMWRTRMYRPSFRAWGRGESWYQGQASEVAPLLDKPYVKPV
                     VVLISPRTFSAAEDFAVAFDAMKRGTLIGEPTGGSTGQPLNIQLPGGGGARICTKRDT
                     YPDG"
BASE COUNT          410 a          428 c          419 g          435 t
ORIGIN      
        1 tccgtctgga taggtgtcgc gtttggtaca aatgcgggcg ccaccaccac ccggcaattg
       61 aatatttagc ggctggccgg tgctgccgcc ggttggttcg ccgatgagtg tcccgcgttt
      121 catcgcatca aaggccacgg caaaatcttc agccgctgaa aaggtgcgcg gactgattaa
      181 caccacgacg ggctttacat atggtttgtc aagtaacggc gcaacttccg aagcctgccc
      241 ctgataccag ctttcgccac gtccccaggc ccgaaacgaa ggtcggtaca ttcgtgtccg
      301 ccacatcgag gtttgaaatg gtttatttgt caaatgagcc agaatgttcc atccatagcc
      361 gctgtttccg ccgccattgt tgcgaacatc aatgatcaac gcatcggttg tcatcagttg
      421 cggaagaact ttggcaaatt cagcttcgat tttgccgtca ttgaagctat ttaacaccag
      481 cagcccaaca ttggactcca gaactttaaa ttccaggatt ggggatggtg gaagtttcaa
      541 tgcggcccat tgttcactgt tcaaacgtgg aactgatttt ttgaaggtct cgccagattc
      601 agttcgaaag gtcaattcaa gggcttgttg aaccggcccc cggagtaatt cataggaaaa
      661 caaacgggtt tccgcatcct gcggagtcga agcggcaaca tacggttgaa taaatttttc
      721 ggcatacgct ttgactggca ttccattgat ttccacgact tccagccctg gcttaatccc
      781 agcttctgta agcttcgaat cccaaacccg gcgcaccata acccggtctt caatcattct
      841 ggtcgaaagc gccgggacgg cataccactg caaagcaagc tcatttggag ggctcaaatt
      901 ggtgtggcca tcttttaatt gggcgaccag ttcggccaaa acccgataat attcatcggt
      961 acttttggtc tgcctgactt ttggaaggta cgccagatag agtttgtccc aatccagttc
     1021 cgggacaaga tcaaaattgg caaagttgaa tttgacctcg gcccacaact ttgagaggcc
     1081 cgcaattttt tcgtcttcgg acagattttc acgatagggc gactggagcg ccgggttgtt
     1141 ccacatcaaa gtcagccgct tttcgttgcg ggtgcacgca tccagtgtga tttgccagcg
     1201 tggatcttcg cgcaatgact gaaaatccgt gtcctgtttc aactgggctg agttaccata
     1261 tccaagcttg agcgcccggt tgagcagatc gaaggcatca tctttccggc ctgccagcgc
     1321 acaggcgcac gccgcgttat ataacaaccc ggaatcatcg gcttggagtt tggtcgctgc
     1381 cacgaaaagc tcggcactcc gggcataatc ttttttcgcg taggcctgtt cagcctgggt
     1441 taccagcgca tcaggatttt gttgggcatt tatcccaact ggttgaagca tcaaccaaac
     1501 tacaaatatc atcagggcaa tgagttttcg tttcacaggt tccttccttt ggagaataag
     1561 aaaattcagg tgagagacga taagaacaag atgttttgga gcttttggcc gggtaagtga
     1621 aacaatcccc attctggttg caacaattct tggtgtggaa tgaaaccaga ggattccaga
     1681 atcgagcaaa at
//
LOCUS       DAOQDO010001626         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77224_length_1691_cov_3.786675, whole genome shotgun sequence.
ACCESSION   DAOQDO010001626 DAOQDO010000000
VERSION     DAOQDO010001626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77224_length_1691_cov_3.786675"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKZ53_21300"
     CDS             <1..248
                     /locus_tag="PKZ53_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43033.1"
                     /translation="FILNSSFSILHSSFFILPPATFSGQIFRFLPNYPPSFLQLTSPW
                     RLLILEGTRCAPSGMIAAALPDQEVRAVQFSLSCQPD"
     gene            397..1074
                     /locus_tag="PKZ53_21305"
     CDS             397..1074
                     /locus_tag="PKZ53_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43034.1"
                     /translation="MNEQQSRLINSEVSVVWDLNREEWDAVARECKPVFLSRIEIAVT
                     SGFFATAVAIFIGLVVTILSLIGVNTPGIGTTLGVLAAVWVGTFLVTSLVIWSVDFFE
                     NLFAIRPKVGETPHCVVSTEGLMVGNRFYFWNKREDRRWHPYAFHHAFAMFDPTSKDT
                     GVSALQVWVTHWFGLKRMVVPVPKDRHREATRLISNLNKYIKHAPPAETIPFPVPQAS
                     SSQKYTA"
     gene            1299..>1691
                     /locus_tag="PKZ53_21310"
     CDS             1299..>1691
                     /locus_tag="PKZ53_21310"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNJ43035.1"
                     /translation="MTNPTSPSTTFFTRLSWLQKTVVATLIIGCLSFGALVADAYAQP
                     ERAKERRDRVLQGFDPQVLMNALKIRQEVEAIRTEVNLTDDQKAQVRAIVEETAPQAK
                     ALRETLRSSREAVKTQLVTNPNDSAALDA"
BASE COUNT          393 a          450 c          393 g          455 t
ORIGIN      
        1 tcttcattct caattcttca ttctcaattc ttcattcttc attcttcatt ctcccgcctg
       61 cgaccttttc tggacaaatt tttcgattct tgccaaatta cccgccatca tttttacaac
      121 ttacgtcacc ctggagactt ttaattctgg aaggaacgcg atgcgcacct tccggaatga
      181 tcgcggctgc acttcctgat caggaagtgc gggccgttca attcagtttg agctgtcaac
      241 ctgattgacc gccagctttg actttgttga atctcaaatc tgtaaccagt tcatcagtca
      301 caagttgcgt caacttgact gcttcaatgc tttatcttct caggggactt atctttttcg
      361 acatgatttt tttcttgtcc aaaggagctt tcagcaatga acgaacaaca gtcccgttta
      421 atcaattcag aggtatcagt cgtgtgggat ctcaatcgtg aagaatggga tgccgttgcc
      481 agagagtgca agccagtctt tctttcaagg atcgaaattg cggtgacttc tggttttttt
      541 gctacggctg tggcgatttt tattggcctc gtggtcacca tattgtcgct gattggcgtc
      601 aatacacctg gaattggaac tacgcttggc gttctggcag cagtttgggt cggtaccttt
      661 ttggtaacca gcctggttat ctggtcggtt gattttttcg agaacctgtt tgcgattcgt
      721 ccaaaggttg gtgagacgcc gcactgtgtg gtgtcaaccg aagggctgat ggtgggcaat
      781 cggttctatt tttggaataa gcgcgaagat cgccgctggc acccctatgc ctttcaccat
      841 gccttcgcca tgtttgaccc aaccagcaaa gatacgggag tgagcgcgct ccaggtgtgg
      901 gtaacccact ggtttggcct caaacggatg gtggttccgg ttcccaaaga ccggcatcgt
      961 gaagcaacac gcctgatttc caacctcaac aagtacatca aacacgcccc tccggctgaa
     1021 acaatcccgt tccctgtgcc ccaggcatcg agttcccaaa aatatacggc ataagaaata
     1081 tgggtggagg cgacggtttg tctcattgac ggtcgctttc acccagccca caaaccgctc
     1141 gtgattgact ttccgtcatt catcagtcag aaccggtcac tcaacccaaa gcatgctcct
     1201 tgccggccag accggcgtac tgtacgactg caagcttacc tgagcagcag ggcttgcaat
     1261 cgaaaaacct cttaagaaaa acagtaaagg agcattgtat gaccaaccca acttcaccct
     1321 ccaccacctt cttcacccgt ctctcctggc ttcaaaagac agttgttgcc acgctcatca
     1381 ttggatgttt gagttttggc gccctggtgg ctgatgccta tgcccagcct gaacgcgcca
     1441 aagaacgccg ggaccgtgtc ttgcagggat ttgatccgca ggtattgatg aacgctttga
     1501 aaattcggca ggaagtcgaa gccatccgaa ctgaagtcaa tctcactgac gatcagaaag
     1561 cccaggttcg ggccattgtt gaagaaaccg ccccacaggc aaaagcactc cgtgaaacgt
     1621 tgcggagttc acgggaagcc gtcaaaaccc agcttgtgac caatcccaat gatagtgctg
     1681 cgcttgatgc c
//
LOCUS       DAOQDO010001627         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77225_length_1691_cov_3.734719, whole genome shotgun sequence.
ACCESSION   DAOQDO010001627 DAOQDO010000000
VERSION     DAOQDO010001627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77225_length_1691_cov_3.734719"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKZ53_21315"
     CDS             complement(<1..566)
                     /locus_tag="PKZ53_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43036.1"
                     /translation="MREPYLLTPSARLVELLMVADNLRQQLDAYRPVKAKEHLWSVLQ
                     EKLRAEWTYHSNAIEGSTLTLGETIFFLQYGLTVEGKPFKDFLDARNHADAIDLLFDF
                     VRDDRPLSELLLKELNSLLLLGVTYTKAIDQFGNPSRKPATPGQYKQLPNTVLRPDGL
                     IHEYVDPLWVVEPNDERNLGLSRPSQASP"
     gene            817..>1691
                     /locus_tag="PKZ53_21320"
     CDS             817..>1691
                     /locus_tag="PKZ53_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43037.1"
                     /translation="MSTNSVVNETGLALPINTNWKLQVWIGVGLVVLIAVVYGQAWQF
                     GFVRWDDNEYVYENEHVIAGLTLKGVTWAFTTLYSSGYWHPVTWLSHMLDCQLFGVHP
                     GWHHVTNITIHAINTLLVLGIVNQMTRRQWCAAATAALFAIHPIHVESVLWLSERKDL
                     LCTLFELLAIGTYLRYGTQTNSWVRYLTVIGLTVLSALSKPSMVALPFLLFLLDFWPL
                     KRIDLPQTGHWKLFWVQGWQLLWEKLPLIVISLMIMLVTYVGTHQGLIAAKFGIPRSS
                     TATYVESTETPFSHQ"
BASE COUNT          414 a          404 c          408 g          465 t
ORIGIN      
        1 ggactcgctt gactcggcct tgaaaggccg agattgcgct cgtcgttcgg ttcaactacc
       61 caaagcggat caacatattc gtgaatcagg ccgtccgggc gtaaaacagt gtttggcaac
      121 tgcttgtatt ggccgggagt ggcgggtttt cgcgaagggt tcccaaattg atcaatggct
      181 ttggtgtagg taacccccag gagcaacagg ctgtttaact ctttgagcaa caactcactc
      241 aatggacgat catcccgaac aaagtcaaag agtaaatcaa tcgcgtcagc atgatttctg
      301 gcatccagaa aatctttgaa tggtttccct tcaaccgtga gaccatattg cagaaaaaaa
      361 atggtttcgc ctaaggtaag cgtgctgcct tcaatcgcat tgctatggta agtccattcg
      421 gcccggagtt tctcctgaag cactgaccag aggtgttctt ttgctttcac aggacgatag
      481 gcatcgagct gttgcctgag attgtccgca accatcagta attcaacaag tctggctgaa
      541 ggtgtcagta aatatggttc tctcataggc aaaatgaatg gatcgtttta aggtttagga
      601 tacaggtttg agcgtgtttc acacttatga tcataccatt ttgaaatgaa gcggttgtat
      661 caaaaaaaaa cagccaagct attcattcaa actaacttag tgaactactg actgctggct
      721 accgacgaca aacgggtatc agattcagcc aaattttatc aagaatttga aatatactgc
      781 ctgattgact catctcaggt ttctcgactc gactctatgt caaccaattc cgtggtcaat
      841 gaaactggat tagcactacc gatcaacaca aactggaaac ttcaggtctg gattggcgtg
      901 gggctggtcg ttctgattgc cgtagtgtat ggacaagcct ggcagtttgg gtttgtccgg
      961 tgggatgaca acgaatatgt ttatgaaaat gaacatgtga tcgctggact cacgctcaag
     1021 ggggtaacct gggcgtttac gacgctctat tcatccgggt actggcatcc ggtgacctgg
     1081 ctttcccata tgctggattg ccagttgttt ggggtgcatc cgggatggca tcacgtcacc
     1141 aatattacca tccatgccat taacacattg ctggtgttgg gaatcgtcaa ccagatgact
     1201 cgccgccagt ggtgtgcggc agcaacggct gctttgtttg ccatccatcc aatccacgtt
     1261 gaatcagttc tctggctttc agagcgcaag gacctgctct gtacgctgtt tgaattgctg
     1321 gccattggca cctatctccg ctatggcaca cagacaaatt cctgggtccg ctatctgacg
     1381 gtcattgggc tgacggtgct gagtgcactc tctaaaccga gcatggtcgc actgcccttc
     1441 cttctcttcc tgcttgattt ctggccattg aagcgcattg atctgcctca aaccggtcat
     1501 tggaaattgt tttgggtgca gggatggcaa ctcctttggg aaaaactccc actgattgtg
     1561 atttcgctga tgattatgct ggtgacgtat gttggaacgc atcagggact catcgccgcc
     1621 aaatttggta ttccacgatc ttccaccgca acctacgtcg aatcaactga aaccccgttc
     1681 tcacatcaat t
//
LOCUS       DAOQDO010001628         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77227_length_1691_cov_3.565403, whole genome shotgun sequence.
ACCESSION   DAOQDO010001628 DAOQDO010000000
VERSION     DAOQDO010001628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77227_length_1691_cov_3.565403"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKZ53_21325"
     CDS             complement(<1..401)
                     /locus_tag="PKZ53_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit A"
                     /protein_id="HNJ43038.1"
                     /translation="MTHTDRPAPPTVVVERVHFQRLFEVLHQKGYRTIGPTLRDGAIV
                     YDEIEGVSDLPVGWTDEQEAALYRIKKRTDDAHFGYVVGPHSWKKFLFPPRVKLWSAR
                     KTETGFQVETEAEEIQPMAFIGVRSCELHALT"
     gene            complement(721..>1691)
                     /locus_tag="PKZ53_21330"
     CDS             complement(721..>1691)
                     /locus_tag="PKZ53_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43039.1"
                     /translation="HKESGLTIDVAAVDNKVMLTIPGQSPYALKATPTKDSFGFKGLP
                     DAFNLTFRRGADGKIIGALLKQPQGDLELVRMSGGTTIPTLTVDELMAKVIDAIGGEA
                     TLTQHKTMVTQGTINLESQGFTGKLITKHKWPNLWAESTTFHAIGKTIGATEEYFDGT
                     AGAGLSSFAEPDTYVGKKLISVRLAAELFPELKWKTLYKTVTLKGIGKVNDEDVYIVV
                     KTPEGSDPITEYISAKTFLILRQDVLQTSDSSERIAIPATILFEDYRTVNGMKVPFKT
                     TQRSSEGGDVVFTLDRVSFDEEIPDSLFQTYKKKKKATGWPEKTSR"
BASE COUNT          400 a          391 c          447 g          453 t
ORIGIN      
        1 gtcagggcat ggagttcgca cgaacgaacc ccgatgaacg ccatgggctg gatttcttcg
       61 gcttcggttt cgacctgaaa gccagtctca gtttttcggg cgctccagag cttgacgcgt
      121 ggtgggaata aaaacttttt ccaggaatgc gggccgacga catagccgaa atgggcatca
      181 tcggtacgtt ttttgatgcg atagagagcg gcttcctgtt catcagtcca accgacgggt
      241 aagtctgaca cgccttcaat ttcatcatac acaatcgccc catcccgtag cgtcgggcca
      301 atggtgcgat accctttttg atggagcact tcgaacaacc gctgaaaatg gacccgctcc
      361 acgacgacag tggggggggc tggtcggtca gtgtgagtca tcggttttca cgatgtagtg
      421 gtctggcttc caacaggcca aatcacgaca tcgtcctcct gggaaagcgg caaagtaggg
      481 ttcagggttc agggttcagg aagagacaat tctttaaaga actgaaccat ttctcaatct
      541 gaaaagaaga cccaaaaagg ataaaattga gagttctact tgagtgaagc gtggggcaac
      601 agagcttctg aagtgttgtg aagtcggaag aacagaggca gaaccggcga ttgtcggttg
      661 aggtgtggct acaatcgaaa ccgccgggta attcatatca aaagatgggg ttggaggcaa
      721 ttagcgagat gttttttcgg gccatccagt cgcttttttc ttctttttgt aggtttggaa
      781 gaggctgtca ggaatttctt cgtcaaatga gaccctgtcg agagtgaaga cgacatcacc
      841 accttcgctt gaacgctggg tggttttgaa tggaactttc attccgttta cggttcgata
      901 atcctcaaac agaattgtgg ccggaattgc aatccgctct gagctgtcac tggtctgtaa
      961 tacatcctga cgtaagatca ggaacgtttt ggccgagatg tattcagtaa tcgggtcact
     1021 gccttccggg gtcttcacta cgatatacac atcttcatca ttcactttcc cgatcccttt
     1081 gagcgtgaca gttttataga gtgttttcca tttcaattcg ggaaaaagtt cagccgccag
     1141 gcgaacggaa atcagtttct tcccgacata ggtatccggt tcggcaaagc tggaaaggcc
     1201 agcgccagcg gtgccatcaa aatattcctc ggtggcacca atggttttgc caatggcgtg
     1261 gaaggtggtt gattctgccc acaaatttgg ccatttgtgt ttggtgatca gtttgccagt
     1321 aaatccttga gactcaagat tgatggttcc ctgagtcacc atggttttgt gctgggtgag
     1381 cgtggcttct ccgccaatgg catcaatgac tttggccatg agttcatcca ctgtcagggt
     1441 tggaatggtt gttcctccgc tcattcgaac cagttccaga tcaccttgtg gttgtttgag
     1501 caacgcccca ataattttcc catccgcacc acgtcggaag gtcaggttaa atgcatctgg
     1561 taaacctttg aacccaaatg agtctttggt cggagtggct tttaaggcgt agggcgactg
     1621 gccaggaatg gtcaacatca ctttgttgtc caccgccgca acgtcaatcg tgagccctga
     1681 ttctttgtga t
//
LOCUS       DAOQDO010001629         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77404_length_1689_cov_3.535496, whole genome shotgun sequence.
ACCESSION   DAOQDO010001629 DAOQDO010000000
VERSION     DAOQDO010001629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77404_length_1689_cov_3.535496"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1689
                     /locus_tag="PKZ53_21335"
     CDS             <1..>1689
                     /locus_tag="PKZ53_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ43040.1"
                     /translation="IFIGGIGLARGYVGDRGQGTGDSAEKPGFRVPGSGSESGDRGQG
                     TGDSSKPVSSAFINSSALSPQPPAPGPTPSTLSLRLYKTGDLGRIRPDGTIEFLGRID
                     DQVKIRGYRIEPGEIESVLRKNTAVRDAVVIAREDTPGNKRLVAYVIAQEAVRTEFDL
                     VRSGLQQFLADHLPEHMLPTALVFLSDFPVSPNGKIDRRALPAPERIETANQAAFAAP
                     ETAIQQTLAEVWTQLLGVKQVGINDNFFSLGGHSLLGTQLMSRIRKVFGVEISLRKLF
                     EFPTLAGLAELIDQTQTMTDPATPLQPELPAIERVSRDLPLPLSFAQHRLWFLDQLEP
                     GSAAYNVPAAIRLQRPLNSTALEQAFSELLHRHEVLRTVYHSVNGTPVQKVLPSASGF
                     QLRKTEVSAEFSTLSEFHQKAQLAQQLAAEASQPFDLATGPLVRALLVKLAEHDHIFM
                     VTMHHIVCDGWSIQILVRELITLYLAFSEHKPSPLVPLPVQYADFAHWQQEWFTGEVM
                     QRQVDYWKTQLADCSPLQLPTDFPRPPIQTHRGSKTTFHFSPELSEKLRTLSQSE"
BASE COUNT          392 a          494 c          423 g          380 t
ORIGIN      
        1 atcttcattg gcggcattgg gctggcacgt ggttatgtag gggatagggg acaggggaca
       61 ggggacagtg cagaaaaacc agggttccgg gttccgggtt ccgggtctga atcaggggac
      121 aggggacagg ggacagggga cagttcaaaa ccagtttcat cggctttcat caattcttca
      181 gccctcagcc ctcagccccc agccccaggc ccaacgcctt caacccttag ccttcggctt
      241 tacaaaaccg gtgacctcgg acgcatccga cctgatggca cgattgaatt cctgggccgc
      301 attgatgatc aggtcaaaat tcgcgggtat cgcattgaac ccggtgaaat tgaatcggtc
      361 ttgcgcaaaa acaccgccgt tcgtgatgcg gtcgtgattg cccgcgaaga tacccccggc
      421 aacaaacggc tggtggccta tgtgattgcc caggaagcgg ttcgcactga gtttgatctg
      481 gtacgaagcg ggctccagca atttctggcc gaccacctgc ccgagcatat gctgccgacc
      541 gcgcttgtgt ttttaagtga cttcccggtt tcgcccaatg gaaaaattga ccgccgggcc
      601 ttaccagccc cagaacgcat cgagaccgcc aatcaggccg cttttgccgc acccgaaacc
      661 gcaattcagc aaacattagc cgaagtctgg acgcaactcc tcggagtcaa acaggttggt
      721 atcaacgaca acttttttag cctgggcggg cattccctgc ttggaacgca attgatgtcg
      781 cggattcgca aagtatttgg cgttgaaatt tcgctccgaa aactctttga attcccgacc
      841 ctggctggac tggctgagtt gattgaccag acccaaacga tgactgatcc agcaacgccg
      901 cttcagcccg aacttcctgc aattgaacgg gtttcacgcg acctgccgtt gccgctgtca
      961 tttgcccaac accggctctg gtttttggat caacttgaac ctggcagcgc ggcctacaat
     1021 gttccggcag ccatccggtt gcaaagacca ctcaactcaa ccgccctgga acaggcattt
     1081 tctgaactgt tgcaccgcca cgaagtgctg cgaaccgtgt atcactcggt caatggtacg
     1141 ccagttcaaa aagtgctccc atcagcgtct ggctttcaac tgcgaaaaac tgaagtctcc
     1201 gctgaattct cgaccctttc agaattccac caaaaagccc agttagcaca acaacttgcc
     1261 gccgaggcca gccagccgtt tgacctcgcc acaggaccgc ttgtgcgggc attgctggtg
     1321 aaactggctg aacatgacca catcttcatg gtcaccatgc accacattgt gtgtgacgga
     1381 tggtccatcc agattctggt gcgcgaactg atcacgctct atctggcttt ttctgaacat
     1441 aaaccttcgc cactggtacc gttgccggtt caatatgccg attttgctca ctggcagcag
     1501 gaatggttta ccggcgaggt gatgcagcgt caggttgact actggaaaac acaacttgcc
     1561 gattgcagcc cgctccagtt accaaccgat tttccacgtc cgccgatcca gacccatcgt
     1621 ggatcaaaga caacctttca tttttcgccc gaactttcag aaaagctcag aaccttgagc
     1681 caatcagaa
//
LOCUS       DAOQDO010001630         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77426_length_1689_cov_2.581395, whole genome shotgun sequence.
ACCESSION   DAOQDO010001630 DAOQDO010000000
VERSION     DAOQDO010001630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77426_length_1689_cov_2.581395"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1689)
                     /locus_tag="PKZ53_21340"
     CDS             complement(<1..>1689)
                     /locus_tag="PKZ53_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43041.1"
                     /translation="SVRNGAGTGDPRVRYDRLTQRWFVMVFNTASSNNRILIAVSSGP
                     TISSAASFTFFFFQHNTVSPAGDNGAFADFPTMGVDANALYIGVNLFTFSYIGSTGFV
                     VRKSSITGAGPIVVSAFRNLVPGGTGEGPYTPQGVDNTDPAATEGYFLGISNTTAGRL
                     VFRRVSNPGGTPTLSADINLTVPTTASPILAPAQGSSIAINTVDDRLNEARIHRDQIS
                     GVTSLWTAHNIQVNSSGVASTSGGRNGARWYQIGNLTGTPTLLQSGTVFDSAASNPRS
                     FYFPAVAMSGQGHAAIAYSFSAANTFVGAATNGRLRTDATGTMQSQTIIQNGLASYQR
                     SDGSRNRWGDYSHTSVDPNDDMTLWTVQEYADSPANTWAVRLTRLNAPPPATPTCGST
                     TTVAPGTSNVNLIVSGTSSSGSGFFDPGTGFANRIQATVNGGGVTVNSVTVNNPTQLT
                     LNISVSGGATAGARTITVTNPDGQQATGGGCLTIGTACANPPTTADAGNPQTLCLPTQ
                     ATLAANTPSVGTGLWSVVSGPSTSAAQFSNTGSPTSTFTPAGGAGVYTLQWSITS"
BASE COUNT          358 a          436 c          512 g          383 t
ORIGIN      
        1 actggtgatt gaccattgca gggtatatac cccagctcca cccgccggtg tgaacgtcga
       61 ggttggtgaa ccggtgttgc taaactgtgc ggctgaagta cttggaccgc tcaccactga
      121 ccacaggcca gttccgacac tcggcgtatt ggctgccagt gttgcctggg tcggcaagca
      181 caacgtttga ggattgccgg catccgccgt ggttggtgga ttggcacagg cggttccaat
      241 cgtgagacag ccgccgccgg ttgcctgctg gccatctgga ttggtgactg taatggttcg
      301 ggcgccggct gtggcaccac cagaaaccga gatgttgagc gtgagttggg tcggattgtt
      361 gacggtgacg ctgttgacgg tgacgccacc accattgacc gtggcctgaa ttcgattggc
      421 aaacccggta cccggatcga aaaagccgga cccactgctt gatgttccac tgacaatcaa
      481 attgacgttt gaggtgcctg gcgccaccgt ggtggttgat ccacaggttg gtgtggctgg
      541 cggtggggca tttaagcggg ttaacctgac cgcccaggtg ttggctgggg aatcagcata
      601 ttcctggact gtccacaatg tcatgtcatc attggggtca acgctcgtgt gtgagtaatc
      661 tccccatcgg ttgcgggatc cgtcgctccg ctgatatgac gccagcccgt tttgaatgat
      721 agtttgactc tgcatcgtgc cagtcgcatc ggtgcgcagg cgaccattgg tggccgcacc
      781 aacaaaagta tttgcggcag aaaagctata ggcaatcgcc gcatggccct ggccagacat
      841 ggcaactgct ggaaaataaa agcttcgggg attgctggct gctgaatcaa aaaccgtgcc
      901 tgattgcagc agtgtcggag ttcccgtgag gtttccaatt tgataccacc gggcgccgtt
      961 tcgtccgcct gaagtgctgg cgaccccgct actgttgacc tggatgttat gcgccgtcca
     1021 caaactggta acgccgctga tttggtcacg gtgaatgcga gcttcgttga gccggtcatc
     1081 aactgtgttg atggcgattg aacttccttg cgctggggca aggattgggc tggccgtagt
     1141 tggaacggtg agattgatgt cggctgataa ggttggtgtt ccaccagggt ttgaaacccg
     1201 gcgaaacaca agccgacctg cggtggtgtt gctgatgccg agaaagtagc cttcagtcgc
     1261 ggctggatca gtgttgtcaa caccttgtgg tgtataggga ccttcaccgg ttccccctgg
     1321 caccaggttt cgaaaggctg aaaccacaat tggacccgcc ccggtgatcg aactcttgcg
     1381 aaccacaaac ccggttgatc caatataaga aaaggtaaac aggttgacgc cgatatagag
     1441 tgcattggca tcaaccccca tggttgggaa gtcggcaaac gccccgttgt caccggctgg
     1501 cgagaccgtg ttgtgctgga agaagaaaaa cgtaaagctc gcagcactgg aaatggtcgg
     1561 accgctgctg accgcgatca aaatgcggtt gtttgaactg gccgtgttaa aaaccatcac
     1621 aaaccagcgt tgggtgaggc ggtcatatcg aacgcgtggg tcaccggttc cagccccatt
     1681 ccgaaccga
//
LOCUS       DAOQDO010001631         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77514_length_1688_cov_2.603184, whole genome shotgun sequence.
ACCESSION   DAOQDO010001631 DAOQDO010000000
VERSION     DAOQDO010001631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77514_length_1688_cov_2.603184"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKZ53_21345"
     CDS             complement(<1..649)
                     /locus_tag="PKZ53_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43042.1"
                     /translation="MAAYSTTTLRTRYHDALVDWQKKNFPEMEILMKYWDKYFPGQTP
                     FQLVAKVGNLKSDTIEFGKFAGQKRFEHAGDMVGNMFYSARDIIKAQCSTELGSIQQH
                     RMTLESAISDEAKFAMLRVQAEELRHAYQMFWVLEHDQSWKKPGHGDVAEETIEQLLS
                     MELGSHVLDAFNIEFNRFLDNVMFTAVIDLVGKYQLDMQKVFSYGPVARSMGPMFS"
     gene            complement(878..>1688)
                     /locus_tag="PKZ53_21350"
     CDS             complement(878..>1688)
                     /locus_tag="PKZ53_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43043.1"
                     /translation="RGGQFTLVGRYRNNDDQRATIRLSGMVQNKEQSFAYEGNLFGRT
                     VPGNDFLPKLWATRRVGALMDQIRLNGENKELVDEIVTLGTRHGIVTPYTSYLATETN
                     QPPPPAPMIGGGRQNAATKPGYQAPRKDPFADEAIVRQSNNEAGAGKKKAGREPNDPF
                     ASAPRSQAVSGAEAVRESKDTAKLRDADKAENIQSATVRTIKNKRFYLVDAVWIDADV
                     TADDKIPTVDLKFGSDEYFALLKQKPELADYFALGVRVTVFFEGKIYRVTD"
BASE COUNT          409 a          409 c          415 g          455 t
ORIGIN      
        1 cgctaaacat cggtcccatg gatcgggcaa ccgggccata gctaaagact ttctgcatat
       61 caagctgata tttgccaacc aggtcaatca cggcagtaaa catcacgttg tcgagaaacc
      121 ggttgaattc aatattgaag gcatcaagaa cgtgtgagcc aagttccatt gaaagcaatt
      181 gctcgatggt ttcttcagcc acgtcgccat ggcccggttt tttccaactc tggtcgtgtt
      241 cgagcaccca aaacatctga taggcgtgac gcagttcttc ggcctggacg cgcaacatgg
      301 cgaacttggc ttcatcagaa atagcgcttt ccagcgtcat ccgatgttgc tggattgagc
      361 ccaactcggt gctgcactga gctttgataa tatcgcgagc gctataaaac atattgccaa
      421 ccatatcgcc cgcatgctca aagcgtttct ggccagcaaa ttttccaaat tcaattgtgt
      481 ctgatttgag gttgccgacc ttggcgacca gttgaaatgg cgtttgtccg gggaagtatt
      541 tgtcccagta tttcatcagg atttccattt cgggaaaatt cttcttttgc cagtcaacca
      601 gggcatcgtg gtagcgtgtg cgtagcgtgg tggtgctata agcggccatg aacgtgaact
      661 cctctgaaac aatgtgaaac aaaataaatg gttaaaattt cgtgagaatc tgacattggt
      721 gtgaatgtga taattttatc gaacgcttgt tcgaaaaagc aagctatatc agagtgatca
      781 agccaaccta tccagcaaaa aaatggcctc cagttgcgtt ggaggccagg ggaaagcatt
      841 tgaaaagact gagttgcaga aaatattcgt gctcaactca atcggtgaca cgatagattt
      901 ttccttcgaa aaagaccgta acgcggactc caagagcaaa gtagtcagcc agttctggtt
      961 tttgcttaag caaggcaaag tactcatcgc tgccaaactt caaatcaacg gttggaatct
     1021 tgtcatcggc tgtaacatca gcatcaatcc aaaccgcatc aaccagatag aaccgtttat
     1081 ttttaatcgt tcgcacggtg gccgactgaa tattctcagc tttgtcggca tcgcgtagtt
     1141 tggcggtgtc tttgctctca cgaacagctt cagcgccgga gactgcctgg gaccttggtg
     1201 cgctggcaaa tggatcattt ggttcccggc ctgctttttt ctttccggca ccagcttcat
     1261 tatttgattg gcgaacaata gcttcgtctg caaaaggatc ttttcgtggc gcttgatacc
     1321 caggtttggt ggctgcattt tgacgcccac cgccgatcat cggggctggc ggcggcggtt
     1381 ggttggtttc ggtggcgagg tatgacgtgt acggtgtcac aatcccgtgc cgggtgccga
     1441 gtgtcacaat ttcatcaacc agttctttgt tttcaccatt gagccgaatt tgatccatca
     1501 gcgcgccaac ccggcgagtt gcccagagct tgggcagaaa atcatttcct ggaactgttc
     1561 gcccaaagag gttgccttca taggcaaagc tttgctcctt gttttgaacc atccctgaga
     1621 gccgaattgt ggctcgttga tcatcgttgt ttcggtagcg cccaaccagc gtgaattgcc
     1681 cacctcgg
//
LOCUS       DAOQDO010001632         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77530_length_1688_cov_2.328843, whole genome shotgun sequence.
ACCESSION   DAOQDO010001632 DAOQDO010000000
VERSION     DAOQDO010001632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77530_length_1688_cov_2.328843"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1688)
                     /locus_tag="PKZ53_21355"
     CDS             complement(<1..>1688)
                     /locus_tag="PKZ53_21355"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ43044.1"
                     /translation="SLTAISALTGKCPTALHDPLTGEIQWREYARQVSPHLAAVLNKG
                     IESHYRDRFKTAREMQEAILSVSSTVAFARPSNGNVLRQTGKFPFQLPKVALSPTPPQ
                     SASLAQFSTVMPQRQSPQPVQPVSPPVPPAASPVALAREDTPQNQNSQPGALSSTNSD
                     AMDLAFWDSIKGSTNPKSYEAYLAKFPSGQFVELAKIRLEEMTSVAQQPVVQTFAFDT
                     VTLDAAGNIIDFKNKKIQGLIEDLGQGITLDMVSLPAGVFQMGSTDQESEKPIHQVRV
                     SAFHIGRFPVTQAQWRVVASYPKVTRDIKPEPSFRKDDHRPVEQISWEDAVEFCERLS
                     QKTGKQYRLPSEAEWEYACRATTSTPFAFGDTVTADLVNYDGNFPFASAPKGKYWNET
                     TRVGSLGAANLFGLFDMHGNVNEWCLDTWNPNYKGAPANATPWINPTESKRVVRGGSW
                     ITGAVNCRSASRASRPMDARNGYTGFRVILVEGSSGTHKSGAFQAASSPAPSPVAPMS
                     PSLPATSMISTPTATLARGSGSRQLSDLPRTRALDIGRGIKIEVVLIPAGEFQM"
BASE COUNT          361 a          424 c          494 g          409 t
ORIGIN      
        1 ccatttgaaa ttcaccggct ggaattaata ccacttcgat tttgatgcca cggccaatat
       61 ccagcgcacg ggtgcgtgga agatcagaca gttggcggct gcctgaaccc cgtgccagcg
      121 ttgcggttgg cgtggaaatc atacttgtcg ctgggagaga agggctcatt ggggcgactg
      181 gtgacggcgc tgggctactg gctgcctgga aggcgcctga tttgtgagtg ccactgctgc
      241 cttcgaccag aataacccga aagccggtgt atccattgcg ggcatccatt gggcgactgg
      301 cgcgactggc agagcgacag ttgacggcac cggtaatcca ggacccacct ctgacaactc
      361 gtttggattc cgtcggattg atccagggag ttgcattggc cggggcgcct ttgtaattcg
      421 gattccatgt gtcgagacac cactcgttga cattgccgtg catgtcaaat aacccaaaca
      481 ggttagcggc gccaagactt ccgacgcgag tggtttcatt ccagtatttg cctttggggg
      541 cggaggcaaa cgggaaattc ccgtcataat tcaccaaatc tgctgtcacg gtatcaccaa
      601 aggcaaatgg ggtgctggtt gtggcccggc aggcatattc ccattcggct tcggagggaa
      661 ggcgatactg tttgccggtt ttctgcgaca gtcgttcaca gaattcaacc gcatcttccc
      721 acgaaatctg ctcgaccggg cggtgatcgt ccttgcgaaa tgatggttca ggttttatgt
      781 cgcgagtcac tttcggataa ctggccacga ctcgccattg agcctgggtc actggaaacc
      841 gtccaatatg aaaggctgac acccgaacct gatggattgg tttttcactt tcctggtcag
      901 ttgagcccat ttggaaaaca cccgctggca acgaaaccat gtcaagcgtg atgccctgac
      961 caaggtcttc aatgaggccc tgaattttct tgtttttgaa gtcaataata tttccagcgg
     1021 catcgagcgt gacggtgtca aacgcgaagg tctgaacaac tggctgctga gccaccgagg
     1081 tcatttcttc cagtcggatt ttggccagtt caacaaattg cccgctgggg aatttcgcca
     1141 gataggcttc atagcttttg ggattggtgc tgcctttgat cgaatcccaa aatgccagat
     1201 ccatcgcatc ggagttggtg ctgctgagcg ctccaggctg agagttttga ttctgcgggg
     1261 tatcttcccg agccaatgct acaggagatg cggctggggg cactggtggg ctgaccggct
     1321 gaaccggttg cggagactga cgttgtggca tcacggttga aaattgggcc agtgatgccg
     1381 attgcggtgg ggttggggag agcgcaactt tgggaagttg aaacggaaac tttccggttt
     1441 gccgcagcac attgccattg gagggcctgg caaaagccac agttgaagaa accgacagga
     1501 tcgcttcctg catttcccgg gcggttttaa atcggtcacg gtagtgcgat tcaatgcctt
     1561 tatttaaaac agcggccaga tgcgggctca cctggcgggc atattcgcgc cattgaatct
     1621 cgccggttaa cgggtcatgg agcgctgtcg gacatttccc ggtcaatgcg ctaatggcag
     1681 tcaggctc
//
LOCUS       DAOQDO010001633         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77639_length_1687_cov_1.840074, whole genome shotgun sequence.
ACCESSION   DAOQDO010001633 DAOQDO010000000
VERSION     DAOQDO010001633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77639_length_1687_cov_1.840074"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PKZ53_21360"
     CDS             complement(<1..158)
                     /locus_tag="PKZ53_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43045.1"
                     /translation="MSSPNTPMLRQYHEIRKQYPGTLLFFRLGDFYEMFYEDAEIGAR
                     ELSITLTAR"
     gene            complement(346..840)
                     /locus_tag="PKZ53_21365"
     CDS             complement(346..840)
                     /locus_tag="PKZ53_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ43046.1"
                     /translation="MHKRNSHCSFCGAPFADDQPWPRVCGNCHNITFRNPIPVAVVLV
                     PVESGLLMIRRGIEPRKGMLALPGGYVNFGESWQQAAAREVLEETGLPLDPAEISLFD
                     VISAPDSTVLIFGQARRRTIAELPSFVPTDETMEMVVLEVLEELAFPLHTQVAEAYFA
                     RAAK"
     gene            complement(867..>1687)
                     /locus_tag="PKZ53_21370"
     CDS             complement(867..>1687)
                     /locus_tag="PKZ53_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43047.1"
                     /translation="SGLVPVLLILLPVWLTAMKRFHNQLEPAVARPKMQQVFATFQIG
                     IAGLLICIFAYSYLTIGSTSRLIEKTASSQFQSNPFERALVSKGSAALPALMGVVERA
                     DLSNYRSQNQLAACLRVLRDIGDPKAAPVVANFLLTASFKPDLYTTEVWAEAAQTLTS
                     LDKKLASPVLQRTIPQLQRQVSKKDYFGTEALIGVFLKCLIETQDREAIRKQLTPEII
                     EFIFEHLDDEQAFTNKLVFREILQALGSPYFKASVELNREWWHNTGKATIQQIF"
BASE COUNT          422 a          384 c          455 g          426 t
ORIGIN      
        1 cgggcggtga gggtgattga aagttcacgc gcaccgattt cggcgtcttc ataaaacatt
       61 tcataaaagt cgcccagccg gaaaaataac agggtgccag gatattgttt gcgaatttcg
      121 tggtactgcc gaagcatcgg cgtatttggg ctgctcataa gtctcgggtt cagggttcaa
      181 ggttcagggt tcagggtttc aaaaccgaaa ccagggactt tagagaggaa gcccattgtg
      241 agcaagagga aaagacaagg tgacaggatg atagggtgac aaggtgacag aataataaat
      301 gtgaggtgag acgcctgatt gactactcgc tccttcactc ctttgctact tcgctgctct
      361 tgcaaaatac gcctcagcca cctgggtatg gagtgggaag gccagttctt caagcacttc
      421 taaaacaacc atttccattg tttcatcagt tggaacgaag ctggggagtt cggcaattgt
      481 ccgtcgcctg gcctgaccaa aaatcaacac ggtgctgtcc ggggcgctga tgacgtcaaa
      541 gagggaaatt tcagccggat caagtggaag acctgtttct tcaaggactt cgcgggccgc
      601 agcctgttgc catgattcgc cgaaatttac atatccgccc ggcaatgcca gcattccttt
      661 gcgtggctcg attccacgcc ggatcatcag taacccgctt tcaaccggaa ccagaaccac
      721 ggcgaccggg attggattgc gaaatgtgat gttgtggcaa ttgccacaga cgcgtggcca
      781 gggctgatca tcggcaaatg gggcgccgca aaaggaacaa tgtgaattgc gtttgtgcat
      841 atcttgggaa ggaatccttt tcttttttag aatatttgct gaatggttgc tttaccagtg
      901 ttgtgccacc attcgcggtt taattcaact gacgctttga aatatggtga gccaagtgct
      961 tgcaagattt cacgaaaaac gagtttgttc gtgaatgcct gttcatcgtc gaggtgttca
     1021 aagatgaatt cgattatttc aggcgtcaac tgcttgcgga ttgcttcacg gtcctgagtt
     1081 tcaatcaggc atttcagaaa aacaccaatg agggcttctg ttccaaaata atcctttttt
     1141 gatacctggc gctggagttg aggaattgtg cgttgcaaga ccgggctggc cagcttttta
     1201 tcaaggctgg tcagtgtttg tgcggcttcg gcccatactt cggtggtgta aagatctggc
     1261 ttgaaggaag cagtgagcag aaaatttgcc acaactggtg ctgctttggg gtcaccaatg
     1321 tctcgaagca cgcgcaggca agctgcaagc tgattttgtg agcggtaatt ggaaagatca
     1381 gcccgctcaa caactcccat cagtgccggc aatgcggctg atccttttga aaccagtgcc
     1441 ctttcaaatg ggttggactg aaactggctg gaggcagttt tctcaatcaa ccggctggtg
     1501 gatccaatcg tcagatagga ataggcaaaa atacatatca gcaatccagc aattccaatt
     1561 tggaaagtgg cgaacacctg ctgcattttg gggcgtgcca cggctggttc aagctggtta
     1621 tgaaatcgct tcattgccgt cagccagacg ggcaatagta tgagcagcac cggaaccagc
     1681 ccgctcc
//
LOCUS       DAOQDO010001634         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77674_length_1686_cov_2.871245, whole genome shotgun sequence.
ACCESSION   DAOQDO010001634 DAOQDO010000000
VERSION     DAOQDO010001634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77674_length_1686_cov_2.871245"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            899..>1686
                     /locus_tag="PKZ53_21375"
     CDS             899..>1686
                     /locus_tag="PKZ53_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNJ43048.1"
                     /translation="MIIALASPSVATTLEDGLKKVKQCLAKAAAQRAEIICFPEAYLP
                     GLRGQDFEVFPFGQAEQERVLQTVAQWSKAYAIAAILGIERLTEAGRQIAACVFDADG
                     QIQGFQTKNQIAPTEDEFYVPGTTRQLFEVNGTKFGIAICHEGWRYPETVRWAAIRGA
                     KIVFHPQHTGSTREGVRLTEWGSTEAPYYEKAMVMRSIENTIYFASVNYGLPFQESAT
                     CLITPSGECQAFLPYGQEGVLVQAINLDEATGLIARRYAPERYQ"
BASE COUNT          474 a          408 c          376 g          428 t
ORIGIN      
        1 ctgatatttc tttgccgctc aaggctggga aatcaggcga accacaagtt gggcggtttc
       61 aatcccgaaa tttggtcatt cccggcgttc aaacaccagt ctcaattcaa ccatcacaag
      121 gtggcgaaga actgtcaaca gagcctaatt ttccccattc gttgagaaaa tcaaacagat
      181 cttctgggtt gggtcctggt acaaccataa ttttaaccac tctctgggcg cgcacttcaa
      241 ttctcacgat tatgctttcc ctgcaatgtg gacaactccc cagtccatat gcccaaaaat
      301 cagtattttc cgcacctgga tctggcccaa cataaggatt cgtcttattt gggcgttgaa
      361 aaacatgatc acccagaaaa aaactgtccc attgtaagta gccaatctca gcttgaacat
      421 ctatttcaga ttgtatttga caggctcgac attgaacggt tgcgtaaaag gtgttgtatc
      481 ttcccatagt tattatctca cggtggtgaa ggattcttat aaaagtaagg gaacccctta
      541 gctttattac cattttgttt ggcaacattg accaggcttc tatcagcaat aataccaatc
      601 agagttcagg gaaatacaat ttattcaatg acatagcctt cacataatac caaagacttc
      661 attccaaaat ggaaagtacc ttaccaaaaa tttccagaca ttttgaacca cgaaatacac
      721 gaaacacacg aaagaaacca aacacttcca aaattcaata tctcaggaaa cttttgacaa
      781 ggtacttata agactgtttt gcaatgaatg ctttatatga gagagtagtt aaataattca
      841 atcaaataag cttcgtgagc tactgactac tgactactga ctacgaactg ttataattat
      901 gatcattgca ctggcatcac cctccgtcgc cacaaccctt gaagatggat taaagaaagt
      961 aaaacagtgt ctggctaaag ctgccgccca gagagcagaa attatctgtt tcccggaagc
     1021 ctatctccca ggtttgcgtg ggcaggattt tgaagttttc ccctttggtc aggccgaaca
     1081 ggaacgtgtg ctccagaccg tggctcagtg gtcaaaagca tacgccatcg cggcaattct
     1141 tggaattgag cgacttaccg aagctggacg ccagattgcg gcctgtgtgt ttgatgctga
     1201 cgggcagatt cagggttttc aaaccaagaa tcagattgcc ccgaccgaag atgagttcta
     1261 tgtgccaggc acgacgcgtc aacttttcga agttaacggc acaaagtttg gcatcgcgat
     1321 ttgtcacgaa ggctggcgct accctgaaac cgtccgctgg gcagccatcc ggggtgccaa
     1381 aatcgtcttc catccgcaac atacaggaag cactcgcgaa ggtgttcgac tgacggagtg
     1441 gggttcaact gaggcgccgt actacgaaaa ggcgatggtg atgcgcagca tcgagaacac
     1501 gatctatttt gcaagtgtca actatggctt gccgttccag gaatcagcca cgtgtctcat
     1561 taccccatcg ggcgagtgcc aggcattttt gccctatggc caggaaggtg tgctggtgca
     1621 ggccatcaat cttgacgaag cgaccgggct gattgcccgg cgatacgctc cggagcgcta
     1681 tcagga
//
LOCUS       DAOQDO010001635         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77838_length_1684_cov_3.263352, whole genome shotgun sequence.
ACCESSION   DAOQDO010001635 DAOQDO010000000
VERSION     DAOQDO010001635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77838_length_1684_cov_3.263352"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..498
                     /locus_tag="PKZ53_21380"
     CDS             244..498
                     /locus_tag="PKZ53_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43049.1"
                     /translation="MKSSYGKKADQWLEQWLKSAINIDTVAQTPTGQYVLVLESPIPP
                     QTRQFFNSPGDVTQVTPSLIFKNQKIHTPATLGLRWLMAR"
     gene            588..1163
                     /locus_tag="PKZ53_21385"
     CDS             588..1163
                     /locus_tag="PKZ53_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43050.1"
                     /translation="MQGPVVEIDVTAICPSLKRLLAQYREFTLGWIEDLDLKKGFRID
                     PTCTGEVMNTLRQNQLKILRVEFSNHETVRLTVDCTDKPAVRRALSSSDEFGNSLQNA
                     WYYIIEDNPNDWQVFLEIRSSKTRFHLGFSLEREIPILKQIIQHRAVIVQAQGELECL
                     GLGNLAMNNLQLIIDKYETRRNQVNNPYQPG"
     gene            1362..>1684
                     /locus_tag="PKZ53_21390"
     CDS             1362..>1684
                     /locus_tag="PKZ53_21390"
                     /inference="COORDINATES: protein motif:HMM:NF024940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ43051.1"
                     /translation="MKKRNSTTEMKEWRRKQALELRKEGWTYREISEALGVTKVAISH
                     WMKQVESEGMAGLEARPHTGSPAKLSEEQQWRLPEYLSHGAEAYGFRGEVWTSRRVAE
                     VIEVE"
BASE COUNT          503 a          377 c          415 g          389 t
ORIGIN      
        1 gaacccggtt tgagatcaac cgaccataaa atcacagcca gcctgatacc agttggtcaa
       61 ttcgccgtgt tttccaaata caggcaacgc cgtcgatttg tgaagacaaa cccgatacca
      121 aaccattttt tttaaatcag gtgcttcttc agttttgact gaaatctaac tgaacttctg
      181 atggtttagc ataaaagttg acctcttcaa ctcaactcaa ttttcttgaa aggtaatccc
      241 caaatgaaat caagctacgg aaaaaaagca gatcagtggc tcgaacaatg gctcaaatca
      301 gccatcaata ttgatacagt cgctcaaact ccaactggcc aatatgtgct ggttctggag
      361 tcaccgattc ccccacaaac cagacaattt ttcaattctc ctggtgatgt cacccaggta
      421 accccatcct tgatcttcaa aaatcaaaag atacatacac cggctacctt gggtttgagg
      481 tggttgatgg cccggtaagt ttccggtttg gacgaccgtt aaaatattcg gagttggaca
      541 tatatgatca aattgcaacc accggggtcc tgaccgttgc cgcaagtatg caagggcctg
      601 ttgttgaaat tgatgtaacc gcaatttgcc catctctcaa acgcctgctc gctcaatatc
      661 gggaattcac cctggggtgg attgaggatc tggatctgaa aaaggggttc cgaattgacc
      721 cgacctgtac gggtgaggtg atgaacactc tccgccaaaa ccagcttaaa atcctgagag
      781 ttgaattttc aaaccacgaa acagtccgac tgaccgtgga ttgtaccgac aaaccagcag
      841 tacggagagc gctttcctcc agcgatgaat tcggaaattc tttgcaaaat gcctggtatt
      901 acattattga ggataaccca aatgactggc aggtatttct cgaaattcgt tcgagcaaaa
      961 cccgcttcca tttaggtttt tccctggaac gggaaatacc aattctgaaa cagattattc
     1021 agcatcgggc tgtgattgtt caggctcagg gggaactgga gtgccttggg ctgggaaatc
     1081 tcgccatgaa taacctccaa ctgatcattg ataagtacga gacgcgccgg aaccaggtga
     1141 ataatccgta ccagcccgga taaacaagca ccagaggatg agccgggatg cgcaatctgc
     1201 aacccggttc aacttggtat gcctcccagc ttgattggga tcgtctcaga aaacggaaaa
     1261 taaagcccgg taaccggtat tgaagagtaa tggaacagaa aactcactta tagagcgttg
     1321 cataatttgc ttaaccccac aatctggtta gaatgagggg tatgaaaaaa agaaatagca
     1381 caactgaaat gaaagaatgg cgacggaaac aagcgctgga attgagaaaa gaagggtgga
     1441 catatcggga aataagcgaa gcgcttgggg taacgaaagt tgcgattagc cactggatga
     1501 aacaagtgga aagtgaagga atggctgggc tagaggcgcg tccacacacg ggaagtccgg
     1561 caaagttaag tgaggagcaa caatggcgat tgccggagta cctctcacac ggtgccgaag
     1621 cgtacggatt tcgaggcgag gtctggacga gccggcgggt ggcggaggtg atcgaggttg
     1681 aatt
//
LOCUS       DAOQDO010001636         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77872_length_1684_cov_2.376304, whole genome shotgun sequence.
ACCESSION   DAOQDO010001636 DAOQDO010000000
VERSION     DAOQDO010001636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77872_length_1684_cov_2.376304"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKZ53_21395"
     CDS             complement(<1..1477)
                     /locus_tag="PKZ53_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HNJ43052.1"
                     /translation="MTTPHTPQPTSGQEIDRGTYEVIRDRLLAQARILAEKTNALNQR
                     RLDLFGSSELAIIGSERIRTVHNCIPRDIINAGRYMLFGYNVFIGLKTETKVEDVLSL
                     HWLEKTETGFAFQPVPPGSDEDFLSHPQFVKDFQELYRYYKEARLLQLRVVEGKILAI
                     FQTGALLKDIKVFRWAVDPKGKVTYLDNRGERDHVYPPSHDFVWTQTTREDQVGGKFP
                     HISILDQVFVETIGGNLTIKIEDNTESGAGIYEEPVEDPRQSLDDAEVHFAKLGGLIL
                     LKILPFREKHYRYFVFNLRTKTVVRIDAIGQACVQLPEDHGIIFPGGYYLQSGEYKTY
                     GESTGMEYKRMVRSPNGEDVLYIFHRRDEGRNVLLPYNKIRKEVALPIDCHGYSKFDD
                     GRLVVLKAPAEPTRVHPMQIWQTPFTSDEYAAAAPSTGTFLESIGNSELVRGISDALS
                     IQKLIVEQTPTTQVYEDLIATVNRTLDSYFWLDTDHAGNIGA"
BASE COUNT          381 a          421 c          438 g          444 t
ORIGIN      
        1 gggcaccaat attgcccgcg tggtcagtat caagccagaa ataactgtcc agcgttcgat
       61 tgaccgtagc aatcagatct tcatagacct gggtggtggg agtttgctcg acaattaact
      121 tttgaatgct gagtgcatca gaaatgccgc gtaccagttc agagttcccg atgctttcca
      181 gaaaggttcc ggttgacggt gccgccgccg catattcgtc actggtaaag ggcgtctgcc
      241 agatttgcat cggatggacg cgagtgggtt cggctggcgc cttgagcacc accagtcggc
      301 catcatcaaa cttgctgtac ccgtgacaat caatcggcag cgcgacttct ttgcgaatct
      361 tgttgtatgg cagtaacaca ttgcggcctt catcccgacg atggaaaata tagagcacat
      421 cctcaccatt gggtgaccgc accattcgct tgtattccat cccggtgctt tcaccatagg
      481 ttttgtactc cccggattgc aggtaatagc cgcctgggaa aatgatgccg tgatcttctg
      541 gaagctgaac acacgcctgg ccaatcgcat caatgcgaac cacagttttg gttcggagat
      601 taaagacaaa atagcggtag tgtttttcac gaaacggcag aattttgagc agaatcaacc
      661 cgcccagttt ggcaaaatgc acttcggcat catcaagcga ctggcgtggg tcttcgaccg
      721 gttcttcata aatgccggcg ccggattcgg tgttgtcttc gattttgatg gtcaggttac
      781 cgccgatggt ttcgacaaac acctgatcca ggattgaaat atgcggaaat ttcccgccga
      841 cctgatcttc acgggtggtt tgggtccaca caaagtcgtg agacggcgga tagacatggt
      901 ctcgctcgcc gcgattatcg aggtaggtca cttttccttt cggatcaacg gcccagcgaa
      961 acaccttgat gtctttgagc aacgccccgg tttggaaaat ggccaggatt ttgccttcga
     1021 ccacccgaag ttgcagcaat ctggcttctt tgtaatagcg atagagttcc tgaaagtcct
     1081 tcacaaactg cggatgactc aggaaatctt catctgaccc cggtggaacg ggctgaaagg
     1141 caaaaccagt ttccgtcttt tcaagccagt gcagactcag cacatcttca actttggttt
     1201 cggtctttaa cccaataaaa acgttgtaac caaagagcat ataccgcccg gcattgatga
     1261 tgtcgcgcgg aatgcaattg tgaacagtcc gaatgcgttc gctaccgatg atggccagct
     1321 ctgaactgcc gaacaagtcc aaccgccgtt gattgagagc attggttttc tcagccagaa
     1381 ttctcgcctg agccagcaac cggtcgcgaa tgacctcgta cgtgccgcga tcaatttcct
     1441 ggccgcttgt gggttgagga gtgtgtggtg tggtcatagg tctttgggtt tgggggtcag
     1501 ggggcagaaa acagggttca gggttcaggg ttcagggttc agaaaacagg gtttggggtt
     1561 cggggttttc gaaagttggt gtggttcaac gcaatagcgt ttactttcac cttgttcttt
     1621 tttcgtcctt tttgtccctt ctgtcctttt tgtcttttca taccctgaac cccgaaccct
     1681 gaac
//
LOCUS       DAOQDO010001637         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_77885_length_1684_cov_2.049110, whole genome shotgun sequence.
ACCESSION   DAOQDO010001637 DAOQDO010000000
VERSION     DAOQDO010001637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77885_length_1684_cov_2.049110"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PKZ53_21400"
     CDS             complement(<1..1349)
                     /locus_tag="PKZ53_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43053.1"
                     /translation="MADTGAFPPDTMGAVGPTQYCVAVNGRFRTFNKTTGVADGNLDI
                     NPDVFFASVLTTPSAGGFNFTTDPQIRFDRLSDRWFIVMLDVPLNSVGDTDKPNRVLL
                     AVSNTATITSSTAWTLYQFQGDATLFTDYESLGIDASALYIGGDMFTLAGAFNSTKGF
                     VVRKAEMLTASPLVVTAFSNLATGAAAGPFAPRGADNYDPTNTGPTATGYFVGVDTLT
                     FSTLMFRRVTNPAGTPTISPNISVTGPTTSSSNNAPQQGSSIRLDTLDDRLYAAHIRN
                     GRLWTAHNIRVNASGVANTGASARHAVRWYEFQNLGTTPILVQSGTQFDNTVTNPRHH
                     FIPSIMVSGQGHAAMGCSTSGSTQFAGAFTNGRLVTDMPLGTLQAGVVYQAGAANYNP
                     ADGSSPHRWGDYSYTCLDPCDDMTMWTIQEFANATNSYGVRAVKLLAPAPTVTACGTA
                     "
BASE COUNT          356 a          480 c          477 g          371 t
ORIGIN      
        1 gctgttccac aggcggtcac ggtcggcgcg ggtgccagca acttcacggc ccggacccca
       61 taggaattcg tggcattggc aaactcctgg atcgtccaca tggtcatgtc gtcacaggga
      121 tcaagacagg tgtaggaata atctccccac cgatgggggc tagatccatc tgccgggttg
      181 tagttggcag cgcctgcctg atagaccact cctgcctgga gggtgcccag gggcatatcg
      241 gttaccaatc gtccattggt aaacgccccg gcaaactggg tcgaaccaga ggtactgcac
      301 cccatcgccg cgtggccctg gcccgaaacc ataattgagg ggatgaaatg atggcgcgga
      361 ttggttaccg tattgtcaaa ctgggtgcct gactgaacca ggatcggcgt ggttcccaaa
      421 ttctggaatt cgtaccatcg gacggcatgc cgtgctgagg ccccagtatt ggcaactccg
      481 ctcgcgttca cccgaatatt atgggccgtc cacaaccgac cgttgcgaat atgagccgca
      541 tagagccgat catctaacgt atccagccga atagagcttc cttgttgtgg cgcgttgttt
      601 gaactcgacg ttgtcggccc cgtgacgctg atattggggg aaatggtcgg tgtgcccgcc
      661 gggttggtga cccgccgaaa cataagtgtt gagaatgtga gcgtatcaac cccgacaaaa
      721 taacccgttg cggttggccc cgtgttggtc ggatcataat tgtccgcccc acgtggcgca
      781 aatgggccgg cagcagcccc tgtcgccaga tttgaaaagg ctgttaccac cagcggtgac
      841 gctgtcagca tctccgcttt gcgcaccaca aaccctttcg tgctgttaaa cgcaccggcg
      901 agcgtgaaca tatccccacc gatatagagg gcactggcat ctattcccaa tgattcatag
      961 tcggtaaaaa gcgtcgcatc cccctgaaat tgatacagtg tccaggccgt ggaggacgta
     1021 atcgtggccg tattgctcac tgccagcaaa acacggttgg gtttatcggt gtcaccgaca
     1081 ctgttgagcg gtacatccag catcacgata aaccaccgat ccgacaaccg gtcaaatcga
     1141 atctggggat cggtggtgaa attaaacccc ccggcactcg gtgtcgtcag gaccgacgcg
     1201 aaaaacacat ccgggttgat gtccagatta ccgtcggcaa cccccgtcgt tttgttgaag
     1261 gtccgaaacc gaccattgac ggccacacaa tactgagtcg gccccaccgc ccccatggta
     1321 tctggcggaa aggcgccggt atcggccaac gtggcacctg taaatgatgt gctcgtggtt
     1381 tgcggcgcca atggctgaaa cggtcaaccc cggttgacca ggtcgttgcg atgaattgct
     1441 ttcatccact ggtggccact gcgatacggc tggcgcatcc gggttttgtg gaagatcttt
     1501 cgggcgttcg tattcaagct ccttccggag cggacggggt tttctcggca ggagctgggc
     1561 ctggcgttcc cgaatcatca tttgttccgt cgtctcgatg attccggcgg caccagtcca
     1621 cgggacgccg atcatttctc cactttgtcc aactggaatc gctggcgttg gcacatttga
     1681 ggta
//
LOCUS       DAOQDO010001638         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78106_length_1681_cov_3.547970, whole genome shotgun sequence.
ACCESSION   DAOQDO010001638 DAOQDO010000000
VERSION     DAOQDO010001638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78106_length_1681_cov_3.547970"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKZ53_21405"
     CDS             <1..920
                     /locus_tag="PKZ53_21405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNJ43054.1"
                     /translation="NLKMTSNNERQALVPRGAEVLPNPNGTAPGILIREGTQVIAMLP
                     GPPREMQPMFTNHVRPVLEPLAGGKRLRQRVMRVSGMGESAIDNLIAPVYSRYTNPST
                     TILFNRTDVEIHLTAFAPTDTEADRLIDEVAVQIEEKLGENLFAHQGETLEQVLALKL
                     TVKGYTIATAESCTGGLLAMRLTEVPGSSNYFLQGVVSYSNQSKTDLLGVPAELIETQ
                     GAVSPEVAEAMARGIKHRSGATIGVGITGIAGPDGGSPEKPVGLVYIGLADDVSVTHK
                     KLLLPGDRERIRWLTSQAAMDLVRRRYLL"
     gene            1135..>1681
                     /locus_tag="PKZ53_21410"
     CDS             1135..>1681
                     /locus_tag="PKZ53_21410"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNJ43055.1"
                     /translation="MTTSFVIKPESNESPFDRQERLSWWKQDVLRQARILIIGAGALG
                     NEALKNLALLGAGRLMVVDFDTISASNLSRTLLFRPEDVGRPKAELAAQRTRDLILEP
                     TARIQSLNADLVWDIGLGLFRRMDVILGCVDNDEARLAINRAARSVGMPWINAGIYEL
                     AGSVTVFSGTDHSCFECNITSD"
BASE COUNT          408 a          439 c          440 g          394 t
ORIGIN      
        1 gcaacctgaa aatgacatcc aacaatgagc ggcaggcatt ggtgccacgc ggggcggaag
       61 ttcttccaaa tccaaatggc acagcgccag gtattctgat tcgcgaaggc acacaggtca
      121 tcgccatgct gccaggccca ccacgtgaaa tgcagccaat gttcaccaat cacgtccggc
      181 ctgttttaga accactggcc gggggaaaac gactccgaca acgggtgatg cgggtttccg
      241 gtatgggcga atccgctatc gacaacctga ttgcgccggt ttacagccgt tataccaacc
      301 catcaaccac tattctgttt aatcgcactg atgttgaaat tcacctgacg gcctttgccc
      361 caaccgatac cgaggcggat cgtctgattg atgaagtggc ggttcagatt gaagaaaaac
      421 tgggcgaaaa cctctttgcc caccagggtg agaccttgga acaggtactg gctttgaaat
      481 tgaccgttaa aggctatacc atcgctactg ccgaaagctg cacgggtggc ttgctggcaa
      541 tgcgcttaac tgaggtcccc ggcagttcaa attattttct gcaaggcgta gtctcttatt
      601 caaaccagtc caaaactgat ctgctcggag taccagcaga actgattgaa acgcagggag
      661 ccgtaagccc cgaagtggcc gaggcgatgg cccgtggtat caaacaccgt tcaggtgcca
      721 ccatcggtgt ggggattacc ggcattgctg gccctgacgg cgggagtcct gaaaaacccg
      781 tgggcctggt ttatatcggc ctggcagatg atgtttcagt aacccacaaa aagctgttac
      841 ttcctgggga tcgggagcga atccgctggc tgacctcaca ggcagcgatg gatctggttc
      901 gccgtcggta tctgctttag gattcagggt ttgtgaaaac acaacccgag cgaacgtgac
      961 aaaaaggaca tccctttcga aaaccctgaa ccctaaaccc taacaagatg accaaatgac
     1021 aaggtgacaa ggtgacaagg tgattttttt catccctcat ccctcatccc tcatcccttc
     1081 cgaaacccct gaatcctgaa ccaagcctgt ccttattata tagagtctgt tgttatgacc
     1141 acgtcctttg tcatcaaacc cgaatctaat gaatcgccct ttgatcgtca ggaacgattg
     1201 agttggtgga aacaagatgt cttgcgtcag gcacgtattc tcattattgg ggcgggggca
     1261 ctgggtaatg aagcactgaa aaacctggcc ttacttgggg ctggacggtt gatggtggtt
     1321 gatttcgata ccatttcggc atccaacctg agccggaccc tattatttcg ccccgaagat
     1381 gtgggccgtc ctaaagcgga actggcggca caacgaaccc gtgatctgat tttggagcca
     1441 acagccagaa ttcaatccct caatgccgat ctggtctggg atatcggcct gggtcttttt
     1501 cgtcggatgg atgtcattct gggctgtgtt gataatgacg aggcccgtct ggccatcaat
     1561 cgggcagccc ggtcagtcgg gatgccctgg atcaatgctg gtatctatga actggcaggg
     1621 agtgtgactg tcttctcggg gacagaccat agttgctttg aatgcaacat cacctctgac
     1681 c
//
LOCUS       DAOQDO010001639         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78212_length_1680_cov_3.028923, whole genome shotgun sequence.
ACCESSION   DAOQDO010001639 DAOQDO010000000
VERSION     DAOQDO010001639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78212_length_1680_cov_3.028923"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKZ53_21415"
     CDS             <1..473
                     /locus_tag="PKZ53_21415"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNJ43056.1"
                     /translation="MLAFAGLMLIVATLMLTRKDQSHEVSQSPDAPPSMPKAIGVGMV
                     VGFLTGFLGVGGGFLIVPALVLFAGLEMKDAVGTSLVVIAINCAAGLVGHLSQAHFDL
                     KLTFLVSLLAVTGTLGGTALSKRVRASSLRNGFAVFVIAVALFLVFKNYGAVIG"
     gene            743..1441
                     /locus_tag="PKZ53_21420"
     CDS             743..1441
                     /locus_tag="PKZ53_21420"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNJ43057.1"
                     /translation="MKLFDNLSPRLVVVCLATVFLMEWSASTQAQTVPRPDDSDIQDW
                     NEVQVSKRIHPQVDVMISGALRFGSNLTHFDDGRFGGQMTLRLNKFISFSPGCFYVSS
                     HPEFKTHTPEVRFVEAINFSLPYKRFEISNRHQFEQRNFLTSRPDFSRYRNRVQVEYK
                     LEQRERPVTLIGSAEWFYNLTTGDWFRSRIGGGIRKQLTHQLSVETMYIHQSDSVSTP
                     GVVHIISSIFRVRL"
     gene            complement(1418..>1680)
                     /locus_tag="PKZ53_21425"
     CDS             complement(1418..>1680)
                     /locus_tag="PKZ53_21425"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNJ43058.1"
                     /translation="ATGHPPNPADFTEVRRHCTLPVILGSGVTPENVEIFYSHADALI
                     VGSTLKHNGKWNHPIDSTRAAVFMEKVYRQREDMCLEPDSEN"
BASE COUNT          401 a          402 c          402 g          475 t
ORIGIN      
        1 tcatgctggc ttttgctggc ttgatgctga ttgtggcaac gttgatgttg actcgcaaag
       61 accagtcaca tgaagtctca caatccccag acgccccgcc cagtatgccc aaagcgattg
      121 gagtcggcat ggtggtcggg tttttgaccg gatttttggg cgtcgggggc gggttcttga
      181 ttgttccagc cctggtcttg tttgctggtt tggaaatgaa agacgcagtt ggaacatcgc
      241 tggtggtaat tgcaatcaac tgtgcggctg gattagtggg tcacctcagt caggctcact
      301 ttgatttaaa gctgaccttc ctggtttccc tgctggccgt taccgggacc ctgggaggga
      361 ccgcgctgtc aaaacgcgtc cgggcctcca gcttgcgcaa tgggtttgcc gtttttgtga
      421 ttgcggtggc gctctttctg gtctttaaaa actatggcgc agtgattggg taatcaagaa
      481 acttcataac ttttccctgc tatttcccca catttggaaa tagctgataa aaaacaagtt
      541 gtctgcaagt tcgttcaaat ggtaaaaggg ttttacaaaa taaaaactca cccaatgtag
      601 aaaaaaacaa actggttcag attgagattt gggaattgcc cgtttgaatt cctgagtacc
      661 atcgagaacc aaacccttta accagcaacc agcagcacac tctatgtccc tgcctggttt
      721 tcttttgtcc agactttttc ccatgaaact ctttgacaat ctctcccctc gtctggtcgt
      781 cgtgtgtctg gcgactgtct ttctgatgga gtggagtgcc tccacccagg cccaaaccgt
      841 tcctcgaccc gatgatagtg acattcagga ctggaatgaa gtacaggtct caaagcggat
      901 tcatcctcaa gtagatgtaa tgatatccgg tgcattgcga tttggtagta atcttacaca
      961 ctttgatgat ggtcgttttg gaggccagat gacactgcgt cttaacaagt tcatttcgtt
     1021 ttcaccaggt tgtttttacg tctcctccca tccggaattc aaaactcata ccccggaagt
     1081 tcgttttgtc gaagccatca atttttcgct gccctataag cgatttgaaa tcagcaaccg
     1141 tcaccaattt gagcaacgga acttcctgac cagtcgtcct gatttttccc gttaccgcaa
     1201 tcgggttcaa gttgaataca aactggaaca acgggagcgt ccggtcacat tgattggttc
     1261 agccgaatgg ttttacaact tgacgacagg tgactggttt cggagtcgga ttggtggtgg
     1321 aattcgaaaa caactgaccc accagctctc ggttgaaacc atgtatattc atcagagtga
     1381 cagtgtttca acgccgggtg tggttcatat catcagttca attttcagag tccggctcta
     1441 aacacatatc ttcacgctga cgataaactt tttccataaa cacagccgcc cgagtggaat
     1501 caatcgggtg attccatttt ccgttgtgtt ttaaagttga accaacaatc aaagcatcag
     1561 cgtgcgaata aaagatttcg acattttccg gcgtcacgcc tgaccccaga atgaccggca
     1621 aggtgcagtg tcgcctgact tcggtaaaat cagccggatt gggcggatgt ccagtggcgg
//
LOCUS       DAOQDO010001640         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78309_length_1679_cov_2.769089, whole genome shotgun sequence.
ACCESSION   DAOQDO010001640 DAOQDO010000000
VERSION     DAOQDO010001640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78309_length_1679_cov_2.769089"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1679
                     /locus_tag="PKZ53_21430"
     CDS             <1..>1679
                     /locus_tag="PKZ53_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNJ43059.1"
                     /translation="FFFGRPSEMIRGKVDPPGCWLDASAVLVRQYLAYCFDSATKYQV
                     LTDFPRQARQLVEDLNRPRGQMERLRTWMSDQEGELRRHFLNRFQNNVQADTQSRFLQ
                     ETTTDLLFLRINEAVRDYESQLRDLENARKRLRDQLSKLDAEEKEAKDEIEQEINILN
                     GRLAGLGRTSALEILTDTGLLPNYAFPERGVRFYGAVYNRYRQNSEEINPVELTRSAG
                     VALKELAPANYFYTHGRQFEIQQLAIGNPQHPLIEEWAICGACGHLRRAEELSRPDAI
                     PACPQCGYANQAGSQLEVGQRRQFIEFSKSQALSTMEHYESLASDLSDEREQEFYQTV
                     HSFDLTKEAPVGAVGDEELPFGIEYRASVTMRTINAGFHSDNGTIPFGVNQKVSDQGF
                     KLCRDCGIAFLPGKKTVEDTHRRSCRARRRFEKLKQEGRLENTFQWHSVYLYRELKSE
                     AIRLLLPFADDADVDTLKASLLLGLRLRFEGNPAHLIVVPHTMPEPATGMKRYYLVLM
                     DGVPGGTGYLKSLYQEHNELGREGEGIMHVMRLAKNALETCVCRQLRQDPL"
BASE COUNT          433 a          426 c          425 g          395 t
ORIGIN      
        1 tgtttttctt tggtcgtccg tcagaaatga ttcgcggaaa ggttgacccg cctggctgct
       61 ggctcgatgc ctcagccgtt ttagtcaggc agtacctggc ctattgtttt gacagtgcca
      121 ccaaatatca ggtgttgacc gatttccccc gacaagctcg ccaactggtt gaagacctga
      181 accgacctcg tggccagatg gaacgcctcc gtacctggat gagtgaccag gaaggagagc
      241 ttcgaaggca tttcctcaac cggtttcaaa ataatgttca ggctgatacc caaagccgat
      301 ttcttcagga aacaaccact gacttattgt tcctccgaat caacgaggct gtacgggact
      361 atgagtcaca actccgtgat ctggaaaatg cccggaaacg cttacgcgat cagctctcca
      421 aactggacgc tgaggaaaaa gaggccaaag acgaaatcga acaggaaatc aacatcctga
      481 atgggcgatt ggccggatta ggacgtacct ctgccctcga gattttaact gatactggac
      541 tcttgcctaa ttatgcgttt cctgagcgcg gagtccgatt ttacggtgct gtttataatc
      601 ggtatcgaca aaacagtgaa gaaatcaatc ccgttgagtt gacacgctcg gctggggtgg
      661 cgctcaagga actggcgcct gccaattatt tttataccca tggcaggcaa tttgaaattc
      721 agcagcttgc cattggaaat ccacaacatc cgctgattga agaatgggca atttgtggtg
      781 cctgtggcca cctgcgccgg gctgaagaac tctcccgccc tgacgcaatt ccagcctgcc
      841 cacagtgtgg ctatgccaat caagccggaa gtcaacttga agttggacag cgccgccagt
      901 ttatcgagtt ttccaagtca caagcattgt ccactatgga gcactatgaa agcctcgcca
      961 gcgatctcag tgacgaacgc gaacaggagt tttatcaaac tgtccacagt ttcgacctga
     1021 ccaaagaagc tccagttgga gcagtcggag atgaagaatt accttttgga atcgaatacc
     1081 gtgcctcagt taccatgcgc acgattaatg ctggattcca ttccgacaat ggcacaattc
     1141 catttggggt caatcaaaag gtttcagatc aaggatttaa gctgtgccgg gattgcggaa
     1201 tagctttcct cccaggaaag aaaacagtag aggataccca tcggcggagt tgtcgtgccc
     1261 gacgacggtt tgaaaaactc aaacaggaag gtcgcctgga aaatacattc cagtggcatt
     1321 cagtttatct gtaccgcgaa ttgaagtctg aagccattcg gctgctgctt ccatttgccg
     1381 atgatgccga tgtagacacc ctgaaagcca gtcttctgct tgggttacga ctgcggtttg
     1441 aaggcaatcc cgcacactta atcgtggtgc cccacaccat gcccgagcca gccactggaa
     1501 tgaagcggta ctacctggtt ttaatggacg gagtccccgg tggaaccggg tatttaaagt
     1561 cactgtatca agaacacaac gaactggggc gcgagggtga gggcattatg catgtcatgc
     1621 gactggccaa aaacgccctg gaaacctgtg tctgtcgtca attgcgccag gatccactg
//
LOCUS       DAOQDO010001641         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78662_length_1675_cov_2.719136, whole genome shotgun sequence.
ACCESSION   DAOQDO010001641 DAOQDO010000000
VERSION     DAOQDO010001641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78662_length_1675_cov_2.719136"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..650)
                     /locus_tag="PKZ53_21435"
     CDS             complement(84..650)
                     /locus_tag="PKZ53_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNJ43060.1"
                     /translation="MNEPTDISANLAANIRRLRELRGATQDKLAQLSGLPRPTWANLE
                     SGSANPTVSVLIKAAAALQVSVEELISPPRSEGRLYHATEIPIKKREGVTVRKLLPDS
                     LQNMELDRMELPPRSRMSGVPHRPGTREYLTCETGQVEIALTGEKWILQPGDVFVFRG
                     DQRHSYANPGDEPAIAYSVVLLGVQGPF"
     gene            743..1462
                     /locus_tag="PKZ53_21440"
     CDS             743..1462
                     /locus_tag="PKZ53_21440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ43061.1"
                     /translation="MNYSGKSILITGASRGLGQALAFHLAERGARLALVARTTAPLEE
                     TVQTIRQRGGIAFAIQADVADKRAIYPIVAQAAELAGPVDILINNASTLGPLPMPLLL
                     DTECEDFGQVLETNLLAPFRLTKAVAGSMVLRGGGVILNISSDAATSAYPNWGAYGVS
                     KAALDHLTRIWAAEFDGTPVKVLSVDPGEMDTQMHADALPEADRATLDSPVTVAQKII
                     SHLENLESMASGTRFLASEVL"
     gene            1459..>1675
                     /locus_tag="PKZ53_21445"
     CDS             1459..>1675
                     /locus_tag="PKZ53_21445"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNJ43062.1"
                     /translation="MTPSRWPRSQPLNNRLLVIDGATQSWTDARVADLGRFLNPGDLV
                     IVNDAATLPAAFFARTETRELIEIRLIC"
BASE COUNT          373 a          441 c          460 g          401 t
ORIGIN      
        1 atttcataaa ctattgaaga attgggattt attttctgtg tattccgtgt attccgtggt
       61 ttctttatcc gaaattctat acctcaaaac ggtccctgaa caccaagcaa aaccacgctg
      121 taggcaatcg ccggttcgtc gcccggattg gcatatgagt gacgttgatc accccgaaag
      181 acaaaaacat cgccgggttg aagaatccat ttttcacccg tcagcgcaat ttcgacctgg
      241 cccgtttcac aggtcaaata ctcgcgagtg ccgggacgat gcggcacacc gctcattcgt
      301 gaccggggtg gaagctccat ccggtcaagc tccatatttt gaagcgaatc aggcaggagt
      361 ttgcgcaccg tcacgccttc ccgttttttg attggaattt cagttgcgtg atagagccgg
      421 ccttcagatc ggggtgggct gatgagttct tcgactgaaa cctggagggc agcggcagct
      481 ttgatcagca ccgaaacggt tggattggcc gaacccgatt caagattggc ccaggttggc
      541 cggggaagac ccgaaagttg ggcgagttta tcctgggttg ctccgcgcaa ctcacgcaat
      601 cgcctgatgt tggcggctaa attggctgaa atgtcggttg gttcattcat gacaagaaac
      661 taaccttttg acagtgtatt agcaaggaag tttcattatt ggcaggacct gatacggtat
      721 gggccaccaa aggagatacc gtatgaacta ttcaggaaag tcaattttga tcactggtgc
      781 cagccgggga ctcggtcagg cactggcctt tcacctggct gagcgcggcg cccggctggc
      841 cctggtggcc cgcacgacag ccccacttga agaaaccgtt caaaccattc gtcagcgagg
      901 aggcattgcg tttgccatcc aggcggatgt ggctgacaaa cgagccattt acccaattgt
      961 cgctcaggcg gcggagctgg ctggaccagt tgatatatta atcaataatg ccagcaccct
     1021 ggggccgctt ccaatgccct tgctgctgga taccgaatgc gaagattttg gccaggttct
     1081 ggaaaccaac ctgctggcgc cgtttcgttt gaccaaagcc gtcgcgggtt caatggtttt
     1141 gcgcggtggc ggcgtcattc tcaacatttc gtccgatgcg gctacgtcag cctatccaaa
     1201 ctggggcgct tacggtgtga gcaaggccgc gctggatcat ctgacccgga tttgggccgc
     1261 tgagtttgac ggaacaccag ttaaggtcct cagtgttgat ccaggtgaaa tggatactca
     1321 aatgcacgca gatgccctgc ccgaagctga ccgggcaacc cttgattcgc cagtaactgt
     1381 cgcccaaaaa atcatttccc acctggaaaa ccttgagtcc atggccagtg gaacccggtt
     1441 tctggcttcg gaggtgttat gaccccctca cgctggccac gaagccaacc gctgaacaat
     1501 cggctcctgg taattgatgg agcaacccag tcgtggacgg atgcacgagt ggctgacctg
     1561 ggcaggttct tgaatcctgg cgatctggtc atcgtcaatg atgcggctac gctgcctgcc
     1621 gccttttttg cccggactga aaccagggaa ctgattgaga ttcgattgat ttgcc
//
LOCUS       DAOQDO010001642         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78683_length_1675_cov_2.196296, whole genome shotgun sequence.
ACCESSION   DAOQDO010001642 DAOQDO010000000
VERSION     DAOQDO010001642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78683_length_1675_cov_2.196296"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1675)
                     /locus_tag="PKZ53_21450"
     CDS             complement(<1..>1675)
                     /locus_tag="PKZ53_21450"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43063.1"
                     /translation="PPSIIAAGQTTVTDLSKPIERDLKGGEVHTYQITASASEYFYVV
                     VDQKGIDVVVTVLAPSGEKIAEVDGPYGTQGPEHLEFVTLASGSIRLEVRPLEAGATS
                     GRYELKLMERRAANPKDKDRVAARGLWGEAEILYYQGARQTLQQAVEKYQAALKLYQS
                     IGDVQGQATCLNAIGLCLVSLGQFQQAISFFEQTLPLAASLKDSRGEGYVMLNIASAY
                     SALGEKLTAIRYYLQALPHFEAAHDEYARAKALNGIGAAYAETGELQKAADSYKKALT
                     LREKIEDPTSLAETLSNLGQVSDVLGKKQLALEYYSRAVEILEKTESRIQQTIVLINI
                     GSVYSSLGESKSALQFYEKAFAFAELLGDRRTQAVILNNIGTVWVQLGDPAAALPFFT
                     RSLPLWEAIGNQLGRAATLNNIGKIQAFQGDTSKAKDTFDQALQIRTKLSDRMGQAQT
                     LNNLGLLAAQTQDHLRAIELLTQSLELDTRVGNRWGQSQTLFNLTKSLVAVGKLTEAR
                     AKIEESLKLIEFIRSNAGAQASRSSFSATVHDHFELYIDVLMLQHQANPK"
BASE COUNT          368 a          437 c          426 g          444 t
ORIGIN      
        1 ttttggattg gcctgatgct gtaacatcag cacgtcaatg tagagttcga agtggtcgtg
       61 aaccgtggct gaaaatgacg accttgacgc ctgggccccg gcattggagc gaataaattc
      121 aatcagtttg agagactctt cgattttggc tctggcttca gtgagttttc cgacggcaac
      181 cagcgatttg gtgagattaa agagcgtttg cgattgcccc caccggttgc ccacccgggt
      241 atccagttcc aggctttggg tcaataactc gatagcacgc agatggtcct gggtttgcgc
      301 cgccagcagg ccaagattgt tcagagtttg cgcctgcccc attcgatcag agagctttgt
      361 gcgaatctgc aacgcctggt caaaggtatc tttcgctttg gaagtatctc cctgaaatgc
      421 ctgaattttg ccaatattat ttaatgtagc ggcgcgcccc aactgattgc cgatggcttc
      481 ccacaatggg agcgaacgag tgaaaaatgg caatgctgcg gctgggtctc ccagttgaac
      541 ccagacggtt ccgatgttat tgagaatgac tgcctgggtt cttcggtcac ccagaagttc
      601 ggcgaacgca aatgcttttt cataaaactg caaggctgac tttgattctc caagtgagga
      661 atacaccgac ccaatgttga tcagcactat tgtctgctgg atgcgactct ctgttttctc
      721 caggatttcg acggctcgac tgtagtactc caaagccagt tgttttttac caaggacatc
      781 gcttacctgc cccagattgc tgagtgtttc cgccagactg gttgggtctt cgattttttc
      841 ccgcaaggtt aatgcttttt tgtaggaatc agccgccttt tgcaactcac ctgtttcggc
      901 ataggccgcc ccaattccgt tcaacgcttt ggctctggca tattcatcgt gcgcggcctc
      961 aaaatgtgga agcgcctgca aatagtatcg gatggcggtt aatttctcgc cgagagcaga
     1021 ataggcactg gcgatattaa gcatcacata gccttcgccc cggctgtctt ttaaggacgc
     1081 agcgagaggt aacgtttgtt caaaaaagga aattgcttgt tgaaactgac caaggctcac
     1141 caggcatagc ccaatcgcat tcaaacaggt tgcctgcccc tgaacatccc caattgattg
     1201 atacaacttg agcgcagcct gatatttttc gaccgcctgc tgcagggttt ggcgagcccc
     1261 ctggtaatac agaatttcag cctctcccca caggccacgc gcagcaactc gatctttatc
     1321 ttttggattg gccgcccggc gttccatcaa cttgagttca tagcgtccag aagtcgcccc
     1381 agcttcaagt ggtcgcacct caagccgaat tgagccgctc gccagggtta caaactcaag
     1441 gtgttctggc ccctgcgtgc cataggggcc gtcaacctca gcgatttttt cgccgctggg
     1501 tgccaaaacg gtcaccacga cgtctattcc tttttgatca acgaccacgt agaaatattc
     1561 actggcactg gctgtgatct gataggtgtg gacttcacca cctttgaggt cacgctcgat
     1621 tggttttgag aggtcggtaa cggtggtttg gcctgccgca atgatggagg gcggt
//
LOCUS       DAOQDO010001643         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78805_length_1673_cov_5.382571, whole genome shotgun sequence.
ACCESSION   DAOQDO010001643 DAOQDO010000000
VERSION     DAOQDO010001643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78805_length_1673_cov_5.382571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKZ53_21455"
     CDS             <1..1029
                     /locus_tag="PKZ53_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNJ43064.1"
                     /translation="NPLYNRQLLTDNHQKPMKAACFSEFGLPQEVLKIEELPIPQPGP
                     GEVLVRLQSRPINPADLFQIMGIYGIKPQLPAVGGMEGAGVIEQVGEGVELQIGQKVV
                     PLKTGSGTWQEYVVAKAVQVIPVPDELSVETASTVVVNPLSAYLMTREIMKVPEGEWL
                     VQTAAGSALGQMVIQLSKVYGFKTINLVRRREQFDELTALGADACICTSDEIIARRIQ
                     EITDGAGVKYAIDAVAGKIGSQVANALTIGGTMLVYGALDQEGLLLNPGSMIFKQLII
                     KGFWLSRWIAEAAPEQRLGLIQAVLPLVMSGKIQSPIAARYNLDDITQAVTHAQQAAR
                     GGKILIVG"
BASE COUNT          410 a          446 c          417 g          400 t
ORIGIN      
        1 aatccactct acaaccgaca actcctcact gacaaccatc aaaaacctat gaaagcagct
       61 tgtttttctg aattcggtct tccgcaggaa gtcctcaaaa tcgaagaact tccaattcct
      121 cagccaggac cgggtgaagt cctggttcgc cttcagtcac gcccaattaa cccggctgat
      181 ctttttcaga ttatggggat ctatggcatt aaaccgcagt tgccagccgt cggtggaatg
      241 gaaggtgccg gcgttatcga acaggttggt gaaggcgttg agttacaaat cggtcagaaa
      301 gtcgtaccgc tgaaaaccgg cagcggcacc tggcaggaat atgtcgtggc caaagccgtc
      361 caggtgattc cagttccaga tgagctatca gttgagacgg cttcgaccgt ggtggtcaat
      421 ccgctatcag cctatctgat gactcgtgaa attatgaagg tgcccgaagg cgaatggctg
      481 gttcaaaccg cagccgggtc agcccttggg caaatggtca tccaactctc aaaagtgtac
      541 ggctttaaga ccatcaatct ggttcgccgc cgcgaacaat ttgacgaact gactgccctg
      601 ggagcagacg cctgtatctg tacttctgat gaaatcattg ctcgtcgcat ccaggaaatc
      661 actgacggtg ccggagttaa atacgcaatt gatgccgtag ccgggaaaat cggaagtcag
      721 gtggccaatg ccctgaccat tggcggcacc atgctggtgt atggtgcgct tgatcaggaa
      781 ggtctcttgc tcaaccctgg ctcaatgatc tttaaacaat tgatcatcaa agggttctgg
      841 ctcagtcgct ggattgccga agccgcgccg gaacaacgac ttggactcat ccaggcagtg
      901 cttccactgg tgatgagcgg aaagattcag tctccgattg ccgccagata caacctcgat
      961 gacattaccc aggcagtcac ccatgctcaa caggcagccc gtggcggaaa aatcctgatt
     1021 gttggataag gattggaacg caacagagac catcaaattc ttaccaccgc agaaacacag
     1081 aggagcccag aggaaaatca ttccttcaaa ccctgtgttc ctctgtgtcc ggcggtggtg
     1141 aaaaagtgtt ttttagacga tccacttttt acatctccac tttcgtcaca ggaagctgga
     1201 ttttccgata actcaacgaa tgagattctt gacgaatcca gactcgtttc gtatggttga
     1261 gggcatctac ctcactttct cttcacctca aaactcaaat cttcgctgga ttcttcagac
     1321 acctgcctgc cgtcttcttt tcaccggtaa ggtgtcctac tcagtcctgc cagtccagat
     1381 tggagagtgg agccaacctc agttggtcca cggctgtccg catcgtttgt tttgactcga
     1441 tgttatttcc cgcacccgga caggtactct cctgtcaaca gactcggccc tgaagggccg
     1501 agcttgagtc gtgtacccac gacaccatcc tgacggatgg ctggtcgtgg ttggattggc
     1561 tcaggcttcg gttgaccaga cccccgtgca aatcacggga aacgattggg aagaatatat
     1621 aggtaccagc ggataccgcc tcagtccgct gcgctacggt gacaggttaa aca
//
LOCUS       DAOQDO010001644         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78884_length_1673_cov_2.086527, whole genome shotgun sequence.
ACCESSION   DAOQDO010001644 DAOQDO010000000
VERSION     DAOQDO010001644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78884_length_1673_cov_2.086527"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..>1673)
                     /locus_tag="PKZ53_21460"
     CDS             complement(120..>1673)
                     /locus_tag="PKZ53_21460"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNJ43065.1"
                     /translation="SSDATIDASDQVLARVSVTGLAASGTSNFPTNSVTIPAGAVIGS
                     RFIAVIADSQNAVTEGNEANNTASIGLNVLLPKPDLAVTNLSLSTTSGTPGSTVSVSF
                     NATNRGGAVVSTSLAQIYLSTDSTITVGDTLVGMTTIQSVAPNTSLPATTTVTIPGTL
                     IVGNYFLGVILDPNGQIDEMDETNNTASNAFSITGQPDLVVDSVIVSPTSVAPGATVS
                     INLIIRNQGPVAAGNASHDIVFSNDATIRSGEDVILVSRSVGPLPPNQTVPISVTVVI
                     PTNQTSLGMKFIGVIADSGNAVAEVSETNNTGSTSFMLLDTVAPSGTIIRPNGGEAIA
                     AGRAFETIEWTATDNLSITSQELRLSTDGGASFPTVIMTGLSPEVRTLRWDVPAGINT
                     STARVQIVIRDASGNEGQASSSANFTIAPPPVIVPTPKLKATGKLVFPIAGSNIMPGA
                     KLQVTVNGAVETFDLTVSQKNVIVRNSARSTPGGRSISQLLPKGTSAMLVVQNPNNVT
                     STPFVFVAP"
BASE COUNT          395 a          424 c          446 g          408 t
ORIGIN      
        1 gttgatagag atttttaact tccttgcatg caaagaggcg atagatgaaa aatcatctat
       61 cgcctctggt tttaacttac aaaattgaac tcaccacatt attcaacccg tcgcattcac
      121 tatggagcaa caaacacaaa cggagtcgaa gtcacattgt ttgggttctg aaccaccagc
      181 atcgcggagg tgcctttggg caaaagttgc gaaatcgaac ggcctccggg tgtactgcgg
      241 gcactgtttc gtacaatcac gttcttttgg ctgacggtca ggtcaaacgt ttccaccgct
      301 ccgttgacgg tcacctgcaa ttttgctcca ggcataatat ttgaaccagc aatcggaaac
      361 acaagctttc cagtcgcttt gagctttggt gttggaacaa tgacgggtgg tggcgcaata
      421 gtaaaattgg ctgaactgct cgcctgccct tcatttccag acgcatctcg gatcacgatt
      481 tgaacgcggg cggtgctggt gttaatgccc gccggaacgt cccagcgcag ggttcgaact
      541 tccggtgaga gtcccgtcat aatcacggtt gggaagctcg cacctccgtc ggttgaaagc
      601 cggagttcct gactcgtaat gctcagattg tcggttgcgg tccattcaat tgtctcgaaa
      661 gcccgtccag cggcaatggc ttccccgcca ttcggtcgga tgatggttcc agacggggcc
      721 acggtatcaa gcagcatgaa cgaggtactc cccgtgttat tggtttccga gacttcagcc
      781 actgcgtttc cagaatcggc aatcacgcca ataaacttca ttcccagact ggtttgattc
      841 gttggaatca ccactgtcac actgatcggc acggtttgat ttggaggcaa tggaccaact
      901 gaccgactga ccaggatcac atcttccccg ctccgaatgg tggcatcgtt tgaaaagacg
      961 atgtcgtgtg aagcattccc ggcagccact ggtccctggt tgcggatgat caggttaatt
     1021 gaaaccgtgg cgccaggagc aacactggtc ggtgaaacga tgaccgaatc aaccaccagg
     1081 tcaggttgcc ccgtgattga aaatgcattg gacgcggtgt tgttggtttc gtccatttcg
     1141 tcaatctgcc cgtttggatc aagaatcacg cccaggaaat aatttcctac gatcaatgtc
     1201 ccaggaatcg tcaccgtggt ggttgctgga agcgaggtat ttggggcaac cgactggatg
     1261 gtggtcattc caaccagcgt gtctccgacc gtaatcgttg aatcggtcga gagataaatc
     1321 tgggcaaggg aggtactgac aaccgcgccg ccacggtttg tcgcattaaa gctcaccgaa
     1381 accgtcgagc ctggggttcc cgaagttgtt gaaagtgaaa ggttggtgac agccaaatca
     1441 ggttttggaa gcaacacatt caatccgatt gaagctgtat tgttggcttc attcccttcg
     1501 gtaaccgcgt tttgggaatc agctatcacg gcgataaatc gcgacccaat tacggcgccg
     1561 gcaggaatgg tcacggaatt ggttgggaaa tttgaggttc ccgaggcggc cagaccggtc
     1621 acgctgactc gggccagtac ctgatcactg gcatcaatcg tggcatcaga cga
//
LOCUS       DAOQDO010001645         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_78918_length_1672_cov_3.820037, whole genome shotgun sequence.
ACCESSION   DAOQDO010001645 DAOQDO010000000
VERSION     DAOQDO010001645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78918_length_1672_cov_3.820037"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..>1672
                     /locus_tag="PKZ53_21465"
     CDS             173..>1672
                     /locus_tag="PKZ53_21465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43066.1"
                     /translation="MYDLHVLLDELTILRQENEQLKGYLLEMSPDAVIAVNRDGKIVS
                     VNQHAEQLFGYDRQELLGQAMEVLIPEQYRDVHHLHRSVYSKSPHFRPMGKTNLEFYA
                     LRKDGTVFSADINLNPVQRSGIVLAAIRDISQRKQLEQSLREAKEQAEAAARAKSIFL
                     AGMTHEFRTPLNGILGFIQLLEQSPTLTADQQKYLAAIQRSGEHLLDIITDVLHMARI
                     ETGRIQLQPQVFDLHRFLYELETRTRTRAQLKHLQVTFDISSNLPRLVEGDAEKLRQI
                     LLNLLGNAVKFTVSGKVTLRVDWQDETAEFEVEDTGPGIAPEALPQIFEPFVQCKAGD
                     VVIEGTGLGLAICRKFVELLGGTIQVKSQVGVGSTFRFVIPLQVKEAVDEESSPASLA
                     PTLINYEAPIRLLVVDDKSDDRELLRMLLSRDGFDVRVAANGHEAVSYWQEWFPHLVW
                     MNMRMPVMDGYAATQKIRQLEELCPPAAPNRMFSTKIIALTANVLDDVRH"
BASE COUNT          394 a          424 c          434 g          420 t
ORIGIN      
        1 attcttcatt cttcattctt cattcttcat tcttcattct tcattcttca ttctccattc
       61 tccatcctcc aatcccgtat ggcaattttg gcaaaaataa ggtattgaac ctgcacaccg
      121 cccctttatt cctgcaccag ccctcttgac cttccacttg agtgatgccg ctatgtatga
      181 cttgcacgtt ctgctggatg aattgaccat tctgcgacag gaaaacgagc agttaaaagg
      241 ctatttgctt gaaatgtcgc ctgatgccgt gattgcggtg aatcgtgatg gaaaaattgt
      301 ttcggtgaat cagcatgcgg aacagttatt tggctatgac cgtcaggaat tgcttgggca
      361 agcaatggaa gtcctgatac cagagcaata tcgggatgtt catcacttac accggagtgt
      421 ctattcaaag tcgcctcatt ttcggcctat ggggaagacc aaccttgagt tttatgccct
      481 gcgaaaagat gggactgtct tttcggctga catcaattta aacccggtgc aacgaagcgg
      541 cattgtgctg gctgcgattc gggacatcag ccagcgcaaa cagcttgagc agtccttgcg
      601 tgaagccaaa gaacaggccg aagctgccgc ccgcgccaaa agtatctttt tggccggtat
      661 gacccacgag tttcgcacac cactcaatgg aattctggga tttatccagt tgctggaaca
      721 atctccgacg ctgactgctg accagcaaaa atacctggcg gccatccagc ggagcggaga
      781 gcacctgctc gacatcatta ccgacgtgct gcatatggcg cggattgaaa cgggtcggat
      841 tcagcttcaa ccccaggtct ttgatttgca ccggtttttg tatgaactgg aaacccggac
      901 ccggacccgc gcccagctca aacacctgca ggtgactttt gacatctcat cgaacctgcc
      961 tcgattggtg gagggcgatg ccgagaaact acggcagatt ttgctcaatt tgctgggaaa
     1021 tgctgtcaaa tttacggttt ccggaaaagt gaccctgcgc gttgactggc aggatgaaac
     1081 agcggaattt gaagtcgaag acaccggtcc cggtattgcc ccagaggcat tgccccagat
     1141 tttcgaaccg tttgtgcaat gcaaagctgg agatgtggtc attgaaggca ccgggcttgg
     1201 actggcgatt tgccggaagt ttgttgaact cctgggcggc accattcagg tcaagagtca
     1261 ggttggggtg gggagtacct tccggtttgt catcccgctc caggtcaaag aagcagttga
     1321 tgaggaatcc agcccggcca gccttgcccc cacgttgatc aactatgagg cgccgattcg
     1381 gttgctggtg gttgatgaca aatcagatga ccgggaattg cttcggatgt tgttgtccag
     1441 agacggtttt gacgtccggg tggccgccaa tggccacgaa gcggtttcat actggcagga
     1501 atggttccca catctggtct ggatgaatat gcggatgccg gtgatggatg gttatgcagc
     1561 gacccagaaa atccggcaac ttgaggaact ctgcccacca gccgcaccaa atcgaatgtt
     1621 ttcaaccaaa attattgcac tgaccgccaa tgtgctcgat gacgtgcgac ac
//
LOCUS       DAOQDO010001646         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79012_length_1671_cov_3.209158, whole genome shotgun sequence.
ACCESSION   DAOQDO010001646 DAOQDO010000000
VERSION     DAOQDO010001646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79012_length_1671_cov_3.209158"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1671
                     /locus_tag="PKZ53_21470"
     CDS             <1..>1671
                     /locus_tag="PKZ53_21470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43067.1"
                     /translation="AMFSIVNGVLFKPLPFQQPEELTVVWSLRTKDGKRNPISYPDFQ
                     DWRALNTSFQDLAVGSGAESLTFTTSIEAHHIRAEYISENFFSVLGVSPLIGRAFLAE
                     ENSMTGPNQVVVLNHSFWQRQFGGSPSIIGKTITLNDAPYTVVGVMPEGFRGESDHAD
                     MWLPFCSVTVAFPSDDPANQFDYVTDRRLRWVAGVGRLKPGVSLIQAQTELDTIAARL
                     EAQYPVTNTGFRVQIQTMTDAWVGEYRTTLQALLLSVGLILLIACSNVINLQMSRSSG
                     RQKEMTVRTALGASRWQLVRLLLIESLILTSLGSLLGTGLALWLDEAAVAFLPVKFPS
                     FVSFAVDWSVLGMAIAATVLSTVMTGLFPALALVVRPDIYTALREKTVKSGRTQWFGS
                     AFAVAEIALALMLLVAAGITLKTLVNHQSQPLGFESENLLTLRVHLGAEKYATDEAVS
                     RINRQLETALKGAPRLTQVGLVQSDLPPTGGGYAWHIERQDQPASGPADLMRTSIHRV
                     NAGFFDAFGMRLLRGEGFSDRQTLESQKVAIISESMARTCWGEADPIG"
BASE COUNT          354 a          418 c          462 g          437 t
ORIGIN      
        1 cgccatgttc agcattgtga atggtgtttt attcaaaccc ttgccctttc aacagcccga
       61 agaattgacc gtcgtctggt cgcttcgaac aaaagatggg aagcggaatc caatttccta
      121 cccggatttt caggactggc gtgcgttaaa caccagcttt caggatctgg ctgttggatc
      181 tggggccgaa tcgttgacgt tcaccacctc catcgaagcc catcacattc gagctgaata
      241 tatcagcgag aatttctttt cagtattggg cgtctctcct ctgattgggc gtgcgtttct
      301 ggctgaagaa aactcaatga ctggtcccaa tcaggttgtt gttctcaatc acagtttctg
      361 gcagcggcag tttggcggat caccatccat cattgggaaa acaatcacct taaatgatgc
      421 gccttacact gttgttgggg tgatgcccga ggggtttcgt ggtgaatctg atcatgctga
      481 tatgtggctg ccgttctgta gcgtcacggt tgcttttcct tctgatgacc cggcgaatca
      541 gtttgattat gtgacagatc ggcgcttgcg gtgggtggct ggtgttggac gactcaagcc
      601 aggtgtttca ctgatccagg ctcagactga acttgatacc atcgctgccc gactggaagc
      661 ccaatatccc gtgaccaaca ccggatttcg ggttcaaatt cagacgatga ctgatgcctg
      721 ggttggcgaa taccgcacca cgctccaggc gttactcctg agtgtcgggt tgattctgtt
      781 aattgcctgc agcaatgtga tcaatctgca gatgagtcgt tcttcggggc gacaaaagga
      841 aatgacggtt cggacggcgc tcggtgccag tcggtggcaa ctcgttcggc tgttactgat
      901 tgaaagcctg attttgacca gtctcggcag tttgctcgga actggcctcg cgctgtggct
      961 ggatgaagcc gccgttgcct ttttgccagt caaatttcca agttttgtct cgtttgccgt
     1021 ggattggtcg gttctgggga tggcgattgc cgcaaccgtg ctctcaaccg tgatgactgg
     1081 tttattcccg gcactggcac tggtcgttcg cccggatatt tacactgcgt tacgcgaaaa
     1141 aacggtcaag tcaggccgga cccagtggtt tgggtcggcc tttgcggtgg ctgaaattgc
     1201 gctggcgttg atgctgctgg tggcggctgg aatcacgctg aaaacgctgg tcaatcatca
     1261 atcgcaaccg cttggttttg aaagtgaaaa cctgctcacg cttcgagtcc accttggggc
     1321 tgaaaagtac gccacggacg aagctgtttc cagaatcaat cgccagcttg aaactgctct
     1381 caagggagcc cccaggctga cccaggttgg gcttgttcaa tcagaccttc cgccaaccgg
     1441 cggaggatac gcctggcata tcgaacgcca ggaccagccg gcatcggggc cagccgattt
     1501 aatgcgaacc agtattcatc gggtcaatgc tggttttttt gatgcgtttg gaatgcgatt
     1561 gctcagaggc gaaggttttt cagaccggca aacgcttgaa tcacaaaaag ttgcaatcat
     1621 cagcgaatca atggcccgca cctgttgggg cgaagctgat ccaattggaa a
//
LOCUS       DAOQDO010001647         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79033_length_1671_cov_2.636757, whole genome shotgun sequence.
ACCESSION   DAOQDO010001647 DAOQDO010000000
VERSION     DAOQDO010001647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79033_length_1671_cov_2.636757"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKZ53_21475"
     CDS             <1..390
                     /locus_tag="PKZ53_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43068.1"
                     /translation="KPVERVPYPPLADRQQQYRTGSLKGPTPLVQYLMNEVEVVGIYQ
                     TQKTTGALLKVKGSSRVIGARVGDLFFNGPIQIIRIYDTKKTGDEGGAALLPGQPAGE
                     IECEEVILYDDNHKEYKKYSITYIPRR"
     gene            456..1058
                     /locus_tag="PKZ53_21480"
     CDS             456..1058
                     /locus_tag="PKZ53_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43069.1"
                     /translation="MHKVPGLGHFQLKPWLLTVSILLCGANVAFATPPKSKSPQNSST
                     LSTGETKSNFKAPPKDLFVDESQIRKSNPVELVPNPPLPERQQLYRNGVIKGPTPLVQ
                     YLINEVDIVGMYRTQKSTGAMLKIKGNSTVIGARVGDLFFNGSIQQILVYTDKRQGDE
                     GRPALLPGQPAGEIRCNQVTRYSDDNQQQEAVTLTYIPRR"
     gene            1145..>1671
                     /locus_tag="PKZ53_21485"
     CDS             1145..>1671
                     /locus_tag="PKZ53_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43070.1"
                     /translation="MPSCYRMCMAVALAFIAGWTNIAAAVVPQQAEGDLAMVTALKVQ
                     PADAQKATVILEGTAHFEYTVLRPESRLITVDLYGVDTSKLQPSNPGGGRLIEAIQVK
                     SVGLPGKVAARVEIQLLQPCEVKSQFEQATHTLILELQLQGMMTSDATSLSPALDAKK
                     AVATANGTTIQSIETA"
BASE COUNT          469 a          395 c          414 g          393 t
ORIGIN      
        1 aaaccggttg aacgggttcc gtatccacct ttggcagacc gtcagcaaca gtaccggact
       61 ggttcattaa aaggaccaac tcctctggtt cagtacctga tgaatgaagt tgaagttgtc
      121 ggcatctacc agacccaaaa aacaactggg gcgttgctta aagtaaaagg ctcatcacgg
      181 gtgattggtg cccgggttgg agacttgttt ttcaatggcc cgattcagat tatccgcatc
      241 tatgatacta aaaagactgg cgatgaaggt ggcgccgcac tactcccagg tcagccagcc
      301 ggagagatcg agtgtgagga ggtgattctg tatgatgata accataaaga atacaaaaaa
      361 tactcaatca cttatattcc acgtcgttag gttgcaatcg gaaacggggt cggtttcaca
      421 atagagttca ggcacgtatc gtcaaaggag gaacaatgca caaagtacct ggtttagggc
      481 atttccaatt gaaaccatgg ctgcttacag tcagcatttt gctctgcggg gcaaatgtcg
      541 ctttcgctac acctccaaaa tccaagtcgc cacaaaattc ctcgaccctc tcaacgggcg
      601 agaccaaatc gaattttaag gcgcccccaa aagatctgtt tgttgatgaa tcgcaaatcc
      661 gcaaatccaa cccagttgag ttggtcccaa acccgccact tcccgaacgt cagcaactct
      721 atcgaaatgg ggtgataaaa ggaccgacac ctttggtcca atacctgatc aacgaggttg
      781 acattgtggg gatgtatcgg acccaaaaat caactggggc gatgctcaag atcaaaggga
      841 actccactgt gattggggcg cgggttggag atttattttt caacggatca attcaacaaa
      901 ttcttgtata cactgacaaa aggcagggcg atgagggcag accggctctc ttacccggtc
      961 agccagccgg tgaaataagg tgcaaccagg tgacccgata tagtgatgat aaccaacaac
     1021 aagaagcggt taccttaact tatattccac gtcgttagct ttagaaatga cactgaatcc
     1081 agagcaaagg gtaatgtaag ttctgtgaga acattctttc cgacacattt gaggaggtat
     1141 tcctatgcct agctgttacc gcatgtgcat ggctgtggcg ctagctttta ttgcagggtg
     1201 gaccaacatt gcagcagccg ttgtccccca acaggcggaa ggcgatctgg cgatggtcac
     1261 cgccctgaag gttcaaccgg cagatgccca aaaagcaaca gtgattcttg aaggcaccgc
     1321 ccattttgag tacaccgtgt tgcgtccaga atcacgtctg attacggtgg atctgtatgg
     1381 tgtagataca tcaaaacttc agccatcgaa tccaggtggg gggcgattga ttgaagccat
     1441 tcaggtgaaa tcagtggggt tgcctgggaa agtggctgca cgtgttgaaa ttcagttact
     1501 tcaaccctgt gaagtgaagt ctcagtttga acaagccaca cataccctga ttcttgaatt
     1561 acagctccag gggatgatga ccagtgatgc gacttctctc tcgccagcac tggatgccaa
     1621 gaaagcggtt gcaacagcaa atggaacgac catccagtct attgaaaccg c
//
LOCUS       DAOQDO010001648         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79042_length_1671_cov_2.434406, whole genome shotgun sequence.
ACCESSION   DAOQDO010001648 DAOQDO010000000
VERSION     DAOQDO010001648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79042_length_1671_cov_2.434406"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKZ53_21490"
     CDS             <1..987
                     /locus_tag="PKZ53_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ43071.1"
                     /translation="APKVARWLGDIRSYFPTSVVRVMQKDALERLNLRQMLMEPELLE
                     AVEADVHLVATLVSLSHVMPGKTKDTARMVVRKVVEDLQRKLANPTRQAVLGSLNRAI
                     RNRRPRHNEIDWNRTIKANLKNYQKEYRTIIPEVRIGYGRKRSSLRDIVLCVDQSGSM
                     ATSVVYSSIFAAVLASLPAVSTSLVVFDTAIVDLTEKLQDPVEVLFGTQLGGGTDINR
                     ALAYCQNLIRRPQETILVLISDLYEGGNQEEMRRRIAHLVNSGVQVVALLALSDSGAP
                     CYDHNNAAHFATLGVPAFACTPDLFPDLMAAAINRRDLGEWAATNGIASAQK"
     gene            complement(1060..>1671)
                     /locus_tag="PKZ53_21495"
     CDS             complement(1060..>1671)
                     /locus_tag="PKZ53_21495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNJ43072.1"
                     /translation="SSFFILHSSFFILNSSFSTLHSSFFILMPARLLDQTFLHRGRVF
                     DVSHDSIELESGLRAEIDIVHHNGGAAVLPLFENGDVLLVKQYRHPATEVLTEIPAGR
                     LEPGEDPLLAAERELEEETGWKAGKIEFLTKFYALPGYSTEVLYCYLATDLTPGTTQL
                     DEDEEIHLLRVPFAEALRMVEAGEIKDAKTMMSVLFTARRMTR"
BASE COUNT          413 a          406 c          462 g          390 t
ORIGIN      
        1 gcaccgaaag ttgcccgctg gttgggagac attcgaagtt actttccaac ctcagtcgtg
       61 cgcgtgatgc agaaagacgc gctggaacgg ttaaatcttc gacagatgtt gatggaacca
      121 gagcttttag aagcggtcga agccgatgtg catctggtgg ccacgctggt atcgctcagc
      181 cacgtgatgc ccggaaaaac caaagacacg gcccggatgg tggttcgcaa ggtggttgaa
      241 gacttgcagc gcaaactggc caatccgacc cggcaggcgg ttctgggcag tttgaatcga
      301 gccattcgca atcgtcgtcc acgccataac gaaattgact ggaatcggac gatcaaggcc
      361 aatttgaaga actaccagaa ggaatatcga accattattc ccgaagtccg cattgggtat
      421 ggtcgcaagc gatcatcact gcgtgacatt gtgctctgtg tcgaccagag cggctcaatg
      481 gcgacctcgg tggtgtattc aagtattttt gcggcggtgt tggcgtcgct cccggcggtt
      541 tccaccagtc tggtggtgtt tgataccgcc attgttgatc tgaccgaaaa gctccaggat
      601 ccggttgaag tcctttttgg aacccagctt ggtggtggta ctgacatcaa ccgggcactg
      661 gcctattgcc agaatctgat ccggcgtcca caggaaacga ttctggtgtt gatcagtgat
      721 ttgtacgaag gtggaaatca ggaagaaatg cgccgccgaa ttgcacatct ggtgaattcc
      781 ggcgtgcagg tggtggcgtt gctggcactc agtgacagcg gtgcaccctg ttatgaccac
      841 aacaatgcgg cacatttcgc cacactgggt gtcccggcgt ttgcctgcac tccagattta
      901 ttcccagact tgatggcggc tgcgattaac cgccgtgacc tgggtgagtg ggccgcaaca
      961 aacggtattg cttcagcaca gaagtgaagt gggctttggg ttccgggttc cgggttttca
     1021 aaagagtgcg gtttaaagca acagcgccct tacaccctgt caccttgtca ttcttcgggc
     1081 ggtgaagagc accgacatca ttgttttggc atctttaatt tcgccggctt cgaccatgcg
     1141 gagtgcttcg gcaaaaggaa cgcgaagcaa atgaatctct tcatcttcat caagttgcgt
     1201 cgtgcccggc gttaaatcag ttgccagata gcaatagaga acttcggtac tgtatcccgg
     1261 taaagcataa aactttgtca ggaactcaat tttccccgcc ttccagccag tttcttcttc
     1321 aagttcacgt tcagccgcca ggagcgggtc ctcgcccggt tcaaggcgcc cagcaggaat
     1381 ctcagtgaga acttctgttg ccggatgccg atattgcttc accagcagga catcgccatt
     1441 ttcaaaaagt ggtaataccg ccgcgccgcc gttgtgatga acgatatcaa tttcagcccg
     1501 caggccagat tcaagttcaa tcgagtcgtg cgagacgtca aaaacacgtc cacggtgaag
     1561 gaaggtctga tccaaaagtc gtgcaggcat aagaatgaag aatgaagaat gaagagttga
     1621 gaatgaagaa ttgagaatga agaatgaaga atgaagaatg aagaatgaag a
//
LOCUS       DAOQDO010001649         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79210_length_1669_cov_3.205081, whole genome shotgun sequence.
ACCESSION   DAOQDO010001649 DAOQDO010000000
VERSION     DAOQDO010001649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79210_length_1669_cov_3.205081"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKZ53_21500"
     CDS             complement(<1..887)
                     /locus_tag="PKZ53_21500"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ43073.1"
                     /translation="MANQKDQPPIFRAKRRLPESPLRVDPLDLPARPPSLRVKPKPVQ
                     VEEDAEGALEVRKKPEETALADPLENRQDLAKKTSKVKWGVGIGLALSAATVGGFLLF
                     RMLNRMKVEGLENIPESHENVLYCPNHSSLLDNFALGVGLYIPRMFFNPEYIPINLAD
                     RKNFFGDPASRRFKDRVLRILGEYFFKNLRTFPVDRGKGGLEQVEQWVEMLKSNIVIV
                     FPEGTRSRTGEIGRGKAGVGKMIYDARPTVVPIRLIGTDEVLGVGTLVPNVFRTVRIV
                     IGKPIDLNELMPDPLPEDP"
     gene            1128..1451
                     /locus_tag="PKZ53_21505"
     CDS             1128..1451
                     /locus_tag="PKZ53_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNJ43074.1"
                     /translation="MSLFEKHVFVCVFGKVCPAHGSEAIWQELRTGMKKAGLHEKIRV
                     NKAGCLAQCEHGPTIVTYPEGTWYAGVTLADVPQIIEDHLIGGCPVERLRHPQPELEP
                     VEAEK"
BASE COUNT          426 a          437 c          379 g          427 t
ORIGIN      
        1 ttgggatcct cgggcaacgg atctggcatt aactcattga ggtcaattgg tttaccaatc
       61 acaattcgca cagttcgaaa cacgtttggc accagggtgc cgactcccag aacttcatcc
      121 gtaccaatca accgaatcgg cacgacggtt gggcgggcat cataaatcat tttgccaacg
      181 ccagcttttc cacgtccgat ttctccggtg cgtgagcgtg ttccttcggg aaacacaatt
      241 acgatgttgg acttcagcat ttccacccac tgctcaacct gctccagacc acctttgccc
      301 cggtcaactg gaaaggtgcg caaattcttg aagaaatatt cgccgagaat gcgcaaaacg
      361 cgatctttga accgacgcga ggcaggatca ccaaagaaat tcttgcggtc tgccaggttg
      421 atcgggatgt attccgggtt gaaaaacatc cgtggaatat agaggccgac gcccaaggca
      481 aagttatcaa gcaacgaaga atggttggga caatagagca cgttttcgtg cgactcagga
      541 atgttttcca acccctcaac cttcatgcgg ttcagcattc ggaagagcaa aaacccgccg
      601 acagtggctg cgcttaaggc cagcccaatt ccaacacccc atttaacttt ggatgttttc
      661 ttcgcaaggt cttgacggtt ttcaagcgga tctgctaaag cagtttcttc cggctttttg
      721 cgaacctcca atgctccttc cgcatcctct tcaacttgaa ctggctttgg tttgactctc
      781 aaactgggtg gtcgggcggg taaatccagt ggatcaaccc gcaagggtga ctcaggcaat
      841 cgccgtttcg ctcgaaaaat tggtggttgg tctttttgat tcgccataga atattgtcga
      901 acatcaattc agggttaaga aacacttttt gaaagtactg gctgaaaaaa caggatattc
      961 ttttaacatc ttccgggttc tcacccggaa ctttccatga aaccttgcta atctatacaa
     1021 atttcggtac tgaaacaaac tcttcttgat gcgccaatca cgggttcttt ccaaaggtgc
     1081 acccttctcc aaccagttta ctttgaccca gagtgttttc acagattatg agtctttttg
     1141 aaaaacatgt ttttgtctgt gtttttggga aagtctgccc agcacacggc agcgaagcca
     1201 tctggcagga attgcggacc ggcatgaaaa aagccggctt gcacgaaaaa attcgcgtca
     1261 acaaagctgg ctgcctggct cagtgtgaac acggcccaac gattgtcact tatccggaag
     1321 gaacgtggta tgctggcgtc acactcgcag atgttccaca aattattgaa gaccacctga
     1381 ttggcggctg cccggttgaa cggttgcgtc atccccaacc agaattagaa cccgtcgaag
     1441 ctgagaaata gggttcaggg ggtggcacga ttatctcggc tttcgctttt ggccggcttg
     1501 tccttaaatt tctattttct gaatcctaaa ctctgatttg ttgcttattt tacaggccgt
     1561 gaacgacctg cctcaattca gccaccgctc cgcggcgaga acccaagcaa aaccaaattc
     1621 gaaaaccctg aaccctgaac cctgaaccct gaaccctgaa ccctgaacc
//
LOCUS       DAOQDO010001650         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79212_length_1669_cov_3.186493, whole genome shotgun sequence.
ACCESSION   DAOQDO010001650 DAOQDO010000000
VERSION     DAOQDO010001650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79212_length_1669_cov_3.186493"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKZ53_21510"
     CDS             complement(<1..540)
                     /locus_tag="PKZ53_21510"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNJ43075.1"
                     /translation="MPISSPRTPRIWPTVDLVAARHLEELLSTLCWDLNATGIETVLE
                     SDDQIQLRIFFAPDECPPDLEAQLREGLRTTGESPDGLFSVTIDGTEERDWQEEWKKS
                     YECLVIGSRWLVVPSWKRPEAEANPDFAGRHWIEIDPGMAFGTGTHDTTRMCLEFLEV
                     LPTSPSSIGDIGTGTGILAI"
     gene            complement(584..1474)
                     /locus_tag="PKZ53_21515"
     CDS             complement(584..1474)
                     /locus_tag="PKZ53_21515"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ43076.1"
                     /translation="MGKLNPSSRETGYLKPGQFYGKVSKNYQLSGFTLSELHHETPKR
                     LHRHAHELAYFCLLLEGNYREFFGTKEIVYTPLTIGYHPPDTVHRDEIGNHGGHFFAI
                     EVADQWLERLREYTSIPNSICELHGGELVWLALRLYRECTDFDLYSPLAVEGLILEML
                     ATVARAQPEKTRRPPTWLLQTVDLLHAEFHQNLTITDIAGRVGVHPFHLSKTFRHTFH
                     QPIGDYLQRLRIHFATQQLSQAETKLSDIALAAGFSDQSHFTRVFKTITGTTPKEFRS
                     TLRLGQQLGSGNTENEKNLW"
BASE COUNT          427 a          384 c          432 g          426 t
ORIGIN      
        1 aatggccaaa attccggttc cggttccaat atcgccaatt gaggaggggg aagtcggcag
       61 tacttccaga aactcaaggc acatccgggt ggtatcgtgt gttccggtgc cgaacgccat
      121 ccctggatca atttcaatcc agtgacgtcc agcaaaatcg gggttggctt cggcttccgg
      181 gcgtttccac gagggaacga ccagccagcg cgagccaatc accagacatt catagctttt
      241 cttccattct tcctgccagt ctcgttcttc agttccatca atcgtcactg aaaacaatcc
      301 atcgggagat tcgccagttg tccgcaagcc ttcgcggagt tgagcttcca aatcaggcgg
      361 gcattcgtct ggggcaaaaa agatgcgcaa ctggatctga tcgtcagatt ccaaaacggt
      421 ttcgatacca gtggcattca gatcccagca cagggttgag agtagttcct ctaaatgacg
      481 ggctgcgacc aggtcaacgg tcggccagat tcgaggagtt cttggtgatg aaattggcat
      541 aaggtgtgtg attttcaact ttgaggatag gaattgccgg attttaccag agatttttct
      601 cattctcagt gtttcctgaa cccagttgct ggcccaggcg caaggtgctc cgaaattctt
      661 ttggggttgt gccggtgatg gttttaaaca ccctggtaaa atgactctgg tcagaaaacc
      721 cggcggccag agcaatgtca gacagtttgg tttcggcctg agacagttgc tgggtggcaa
      781 aatgaattcg caagcgctgt aaatagtccc caatcggctg gtgaaatgtg tgacgaaatg
      841 ttttggaaag atgaaacggg tgaacgccaa cacgaccggc aatgtcggtg atagtcaggt
      901 tttggtggaa ttcagcgtgg agcaaatcta cggtctgtag cagccaggtt ggtggacgac
      961 gtgttttttc aggctgtgct ctggcgacgg ttgccagcat ttcaaggatg agtccttcaa
     1021 cagcaagcgg cgaatacaga tcaaagtccg tgcactcccg gtagagccgc agtgccagcc
     1081 agaccagttc tccaccgtgc aattcacaaa ttgaattggg gatggaggta tattcccgca
     1141 atcgttcaag ccactggtca gccacttcaa tcgcaaaaaa gtgtccaccg tggttgccaa
     1201 tttcatcgcg atgaactgta tcaggcgggt gatacccaat tgtcagcggg gtatagacga
     1261 tttcctttgt cccaaaaaac tcccggtaat ttccctcaag caacagacaa aagtaggcca
     1321 gttcatgggc atgccggtga agtcgtttgg gcgtctcgtg atggagttca gacaacgtaa
     1381 aaccagaaag ctggtaattt ttagacacct tgccgtaaaa ctggccgggt ttaagatagc
     1441 cagtttctct ggatgaaggg ttaagttttc ccataaatcc ttcttcggcc tgatgttgaa
     1501 gtgtgattga ttttggattg ataagctggt ggtgagcgta acggaatcaa cgtcaggctt
     1561 gttccttacc accagagcaa ataccagatt aggaatcgcc tgttcatatt tatagaactc
     1621 taaattatca attctaaaaa acttaacaaa atacaagccg ctgattatt
//
LOCUS       DAOQDO010001651         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79325_length_1668_cov_2.300062, whole genome shotgun sequence.
ACCESSION   DAOQDO010001651 DAOQDO010000000
VERSION     DAOQDO010001651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79325_length_1668_cov_2.300062"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1668
                     /locus_tag="PKZ53_21520"
     CDS             <1..>1668
                     /locus_tag="PKZ53_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ43077.1"
                     /translation="AYHPHGGSVHCAILAEAGHGEEDWTTRLYRRVMTPLLVIPKYRI
                     LFLVAVIGLLLASAALVGVKLVTVKMLPFDNKSEFQVIIDMPEGSTLEQTAAITREIA
                     GKLQQVPEVASLQLYVGTASPYNFNGLVRHYFLRQGPNVADIQVNLASAEARKAQSHD
                     IAKRVRPEVQQIAARYGARVKVAEVPPGPPVLQTLVAEIYGPTQKQQISLAKQILEIF
                     EKTPGVVDVDWYVEDDQPKYRFVVDKAKAALNGVSPEQIAASLRLAVNGMPAGLLHQV
                     QEKEDVVILLRMDRGSRSSIQDLQQIKVMGENGNLVPLSQLVTVEETVEDHNIYHKNM
                     MPVVYVTGDVAGSEESPVYAILKLTDAVKKLKIPEGYQIEQFTADQPFSTDRLSVKWD
                     GEWYITYEVFRDMGLAFAAVLVLIYILVVGWFQSLKTPLVIMAAIPFSLVGILPAHAA
                     MGAFFTATSMIGFIAGAGIVVRNSIILVDFIELRLAEGVPLTQAVVDAGAVRFRPMLL
                     TAAAVVVGASVILFDPIFQGLAISLMAGEIASLLLSRMTVPILYYLS"
BASE COUNT          369 a          429 c          480 g          390 t
ORIGIN      
        1 gcgcttatca cccccacggg ggcagcgtgc actgcgcgat cttggccgag gctggacatg
       61 gcgaagaaga ctggacgacc cgactgtatc gccgggtcat gacgccgcta ctggttattc
      121 caaaataccg aatactcttt ctcgtcgcag tcattgggtt gttgctggct tcagccgcac
      181 tcgtcggagt aaaactggtc acggtgaaga tgctgccgtt tgacaacaag agcgaatttc
      241 aggtgatcat tgacatgccg gaaggctcga cacttgaaca aaccgccgcc atcacccgtg
      301 aaattgccgg aaagctgcaa caggtccccg aagtcgccag cctgcaactc tatgtcggaa
      361 cagcgtcgcc ctataatttc aacgggctgg tgcggcatta ctttttgcgg caaggtccaa
      421 acgtagctga cattcaggtc aatctggcct ctgctgaagc gcgaaaagcc cagagtcacg
      481 acattgccaa acgtgtccgc cccgaagttc agcagattgc ggcccgatac ggtgccagag
      541 tcaaggtggc cgaagtccca ccgggtccgc cggtgttaca aacgctggtg gctgaaattt
      601 atggcccgac tcaaaagcaa caaatttcgc tggcaaaaca gattttggaa atcttcgaga
      661 agacgcctgg cgtggtggac gttgactggt acgtcgaaga cgatcagccg aaataccgct
      721 ttgtcgtgga taaagccaaa gccgcactca acggagtctc acccgaacag attgccgctt
      781 cgctccggct ggccgtcaat gggatgcccg ctggtttgct gcatcaggtt caggaaaaag
      841 aagatgtcgt gatcctgctc cggatggatc gtggatcgcg ctcaagtatc caggatttgc
      901 agcagatcaa agtcatgggc gagaacggaa atctggtccc gctcagccag cttgtgacgg
      961 ttgaagaaac cgtcgaagac cacaacatct accataaaaa catgatgccg gtggtctatg
     1021 tcaccggtga tgtcgctggt agcgaagaaa gcccggtgta tgcaattttg aaactaacgg
     1081 atgcggtcaa aaagctcaaa attccggaag gctaccagat cgagcaattt actgccgatc
     1141 aaccattttc gactgaccgg ctttccgtca agtgggatgg cgaatggtat atcacctacg
     1201 aagtcttccg cgatatgggg ctggcgtttg cggcggtgct ggtcttgatt tacatcctgg
     1261 tcgtcggctg gtttcagtcg ctgaaaacgc cgctggtgat aatggccgcg attccttttt
     1321 cactggtcgg gattttgccg gcgcacgcag caatgggcgc gttttttaca gccacttcaa
     1381 tgattggatt tattgcgggc gccgggattg tcgttcggaa ctcaatcatt ctggtggatt
     1441 ttatcgaact gcgactggcg gaaggcgtgc cgctcaccca ggctgtggtt gacgccgggg
     1501 cggtgcggtt caggccgatg ttgttgacgg cggcggcagt ggtcgtcgga gcgtccgtca
     1561 ttttgtttga cccgatcttc caggggctgg cgatttcgtt gatggcgggt gaaatcgctt
     1621 cgttgttact ttcacggatg accgttccga ttttgtatta cctcagcg
//
LOCUS       DAOQDO010001652         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79380_length_1667_cov_4.081266, whole genome shotgun sequence.
ACCESSION   DAOQDO010001652 DAOQDO010000000
VERSION     DAOQDO010001652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79380_length_1667_cov_4.081266"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1667)
                     /locus_tag="PKZ53_21525"
     CDS             complement(<1..>1667)
                     /locus_tag="PKZ53_21525"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ43078.1"
                     /translation="NCRKGLELLQKLQPLTLNDHLVLGKLRNTLALVDAEEGHNQQAV
                     QNCKQALEHWSQSKDGGLMAMGYANLAGAQLAIGTWRQAEESLRLAQTLIQDQNRHTE
                     CMVLTILSALHLRQGKFVEAERSARRAVELAMESGLKAAEGSGWESLGEILLAQGRAE
                     EAITQFRHSLRIFTKLGRRVDLPGLYLRLSEAHLERAQSDTAFDCLLKAQELLKDQPK
                     LHLNALTARLKGRLHIVRNELSQGITSLAQSISLFESISHPYEAACGHLEIGLALAGT
                     DPGRAVSHLETATRVFTELKAEPKLNKARELLASLRETLAPSRRSASALTIDDVVVIE
                     RLNAASGSHELLLRELAAILHNQLQQDVVIFEHIQADHSFKPVVIHGFSDREVEKAAR
                     TLRTSLETGKKVPDGILLKALTDTSHTVGADLQRNFWVFLQGDDSGLPPQFGALDALM
                     RISNMSLELCRLRSLTQSMRKTVSQFVTGSYVTIAGMFCESPSMQQVLSQIQKIRSSD
                     ATVLITGESGVGKELVARAIHQTSTRHSRLFLPFNCASIPAELVESR"
BASE COUNT          356 a          436 c          491 g          384 t
ORIGIN      
        1 accggctttc aaccagttca gccggaatcg aagcacagtt aaacgggaga aagagccgac
       61 tgtggcgagt gctggtctgg tggatggccc gggcgaccag ttctttgccg actcccgact
      121 cgccggtgat gaggaccgtg gcatcagagg agcgaatttt ctggatttgg ctcaggacct
      181 gttgcatcga gggggattcg caaaacattc cggcaatggt gacataactt cccgtcacaa
      241 actggctgac cgttttgcgc atggactggg tcaatgaccg gagccggcac agttccagac
      301 tcatattgga gatacgcatc aaggcatcga gggcaccaaa ctggggtggc agcccggaat
      361 catcgccctg gagaaaaacc cagaaattgc gctgtaaatc agctccgact gtgtgtgaag
      421 tatcggtcag ggctttgagt agaattccat ccggcacttt tttgccggtt tcgagactgg
      481 tgcgcaacgt gcgggcggct ttttcgactt cgcggtcaga aaatccatgg atgacaactg
      541 gtttaaaaga atgatcagcc tggatgtgct caaaaatgac aacgtcttgc tgtaactggt
      601 tatggagaat cgctgccagt tcgcgcaata acagttcgtg tgagccagaa gcggcattga
      661 gtcgctcgat caccaccaca tcatcaatcg tcaaggcact ggctgaccgg cggctgggcg
      721 cgagtgtttc ccgcagagag gccagcaatt ccctggcttt attgagtttt ggctcggctt
      781 tgagttctgt aaaaaccctg gtggcggttt caaggtgtga gacggcgcgt cctgggtcgg
      841 tgccggccag cgccagtccg atttcaaggt gaccgcacgc cgcttcatag gggtggctga
      901 tggattcaaa cagggaaata ctttgggcca ggctggtaat tccttgagag agttcattcc
      961 ggacaatatg cagccgtcct ttgagccgtg cggtcagggc attgaggtgg agtttaggct
     1021 gatctttcag taattcctgg gctttgagca gacaatcaaa ggcagtatca ctctgggcac
     1081 gttccagatg ggcttcgctc aaccgcaggt agaggcccgg aaggtcaacc cggcgtccga
     1141 gtttggtaaa aatccggagg ctgtgacgaa actgggtaat ggcttcttcg gcccgtccct
     1201 gagcgagcag gatttcccca agtgactccc acccggaacc ctcggcagct ttcaatcctg
     1261 attccatcgc cagttcaaca gcccggcggg cactgcgttc ggcttcgaca aatttcccct
     1321 gacgcaaatg cagggcgctc aaaatggtga gcaccatgca ttcggtatgt cggttttggt
     1381 cctgaatcag cgtttgtgcc agccgaagcg actcttcagc ctgccgccag gtgccgatgg
     1441 caagctgggc gccggccagg ttggcatacc ccatggccat caatccacca tctttggatt
     1501 gtgaccagtg ttcgagggcc tgcttgcaat tctggaccgc ctgctggtta tggccttcct
     1561 cagcatcaac cagcgccagg gtattgcgca gtttcccgag caccagatgg tcattgaggg
     1621 tcagcggctg gagcttttgc agcaattcga gtcctttgcg gcagttg
//
LOCUS       DAOQDO010001653         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79464_length_1666_cov_4.473619, whole genome shotgun sequence.
ACCESSION   DAOQDO010001653 DAOQDO010000000
VERSION     DAOQDO010001653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79464_length_1666_cov_4.473619"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1320)
                     /gene="wecB"
                     /locus_tag="PKZ53_21530"
     CDS             complement(148..1320)
                     /gene="wecB"
                     /locus_tag="PKZ53_21530"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNJ43079.1"
                     /translation="MLIHRKRIVLCLGTRPEIIKMAPVYRALTECGSEPIVVHTGQHQ
                     QGVKELYQFFGMPIHHELEIPRHREGLGHLTAQLIETLDQCFGRLKPEVVLVQGDTTS
                     AWAAALVAYYHRIPVGHVEAGLRTHREYEPFPEEKNREAIARLATWNFAPTLQARHNL
                     LAEGIPEAKIHRVGNTIVDAVRWAIPQVSGYSNGHRRLLPGALQLLAAQLDHRKLLLI
                     TAHRRENWGTNIAQISQAVAKLLTQDPSLVAVWPLHANPHVQTTVRQIVPTLPPQVRE
                     RFVLTDPLHYPFLIWLLTNAWLVLTDSGGIQEEASETRTPVLILRNTTERMELVHVGG
                     GKLVGTEPAAILHAVGYLLANPNEYNQMRYFDNPFGDGHAGRYIAEMLLGPLECRA"
BASE COUNT          422 a          415 c          429 g          400 t
ORIGIN      
        1 gtgcataaaa tcagtgacac gggcatttgg attgagggaa atataaccgt tgatccgata
       61 tgaacgggtt aaaataatca ctttggtcat ttcttgtgga ttcataaaag attgaactcc
      121 tcaccttgtc tcagttgcac atcatcatca agcccggcac tcaagtgggc ccaggagcat
      181 ttcggcgata tagcgcccgg catggccatc gccaaatgga ttgtcaaaat accgcatctg
      241 attgtattca tttgggttgg caagcaagta tcctactgca tggagaatgg ctgccggttc
      301 ggtcccaacc agtttaccgc cgccgacatg gaccagttcc atgcgttcgg ttgtgttgcg
      361 taaaatcagc accggggttc ttgtttctga agcttcctcc tgaattcccc ctgaatcggt
      421 caataccagc caggcattgg tcagcaacca gattaaaaat ggatagtgga gcggatccgt
      481 caagacaaag cgttcgcgaa cctggggcgg cagcgtcggt acaatctgac gaaccgttgt
      541 ctgaacgtgc gggttggcgt gcagtggcca aaccgcaacc agcgatggat cctgagtcag
      601 caacttcgct acggcctgac tgatctgggc aatgttggtg ccccaatttt cacgccgatg
      661 ggccgtgatc aagaggagtt ttcgatggtc aagctgggca gccagaagtt gtaatgcccc
      721 tggaagcaat cgccgatggc cattggaata gccgctgacc tgtggaatcg cccaccgcac
      781 tgcatccaca attgtatttc caacccgatg gatttttgct tcggggattc cttctgccag
      841 caagttatgt cgagcttgca gcgtaggcgc aaagttccag gtcgccagcc gggcaatcgc
      901 ctcacgattt ttctcttctg gaaatggttc atattcgcga tgtgttcgca agccagcttc
      961 aacatgacca accggaatcc ggtggtaata agccaccaga gccgccgccc aggcactggt
     1021 tgtatcacct tgaaccagca cgacttctgg cttgagccgg ccaaaacatt gatccagtgt
     1081 ctcaatcaat tgcgcggtca gatgccccag tccttcgcga tggcgtggga tttccagttc
     1141 gtggtgaatc ggcatcccaa agaactgata taactctttg accccttgct ggtgctgtcc
     1201 ggtatgaacc acaatcggct cagacccgca ctcggtcaga gcccgataga ccggggccat
     1261 tttgatgatc tctgggcggg tgcccaggca caggacgatt cgttttcgat gaatcagcat
     1321 cgttggttca gaaatcaagt tggagtgaaa tcagacacag aacagaagag agcggctttg
     1381 atatttggat tggacgccag gaagtcaaag gcccagagca cgtagaaaaa taccttgttt
     1441 gggctgcatc atacaccagg attggtcagt cgtcagtagt tgatgagtca gtcggtgatg
     1501 agtcagttgt cagtcgtcag tcgtcagtcg ataaaaccca gattgttgaa tccatgaaat
     1561 tcttgaaaag aattgttcag aactgactca aatagaacca gatagacctg gcagatactt
     1621 caaaatccca acacttgacg actgacgact gactcataag aaaaaa
//
LOCUS       DAOQDO010001654         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79527_length_1666_cov_1.792055, whole genome shotgun sequence.
ACCESSION   DAOQDO010001654 DAOQDO010000000
VERSION     DAOQDO010001654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79527_length_1666_cov_1.792055"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1666)
                     /locus_tag="PKZ53_21535"
     CDS             complement(<1..>1666)
                     /locus_tag="PKZ53_21535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ43080.1"
                     /translation="NSRFNGPVRDLFPSVESIAEFKVSSFNNSAEFGQVTDLTTTSRS
                     GQNEFHGSAFWFHQNRALNATDPFAPSDPARPGKRLKPALVANSFGGNLSGPISIPNF
                     YDGRNRSFFFFNYEGVRRPNQTLLSEIVPPDAFRAGDLSSISTPLLNPFSGQPFARNQ
                     VPVNPTSARILELLYPRQNQATGAAIQPNFVANVPGAYGVNGFDLRGDHAFSERKTVF
                     TRYSHKNVTDTGTDGTGSYNLQNGTFSRITEVRNLAGSFNWIFTPKVLNEFRAGLSRS
                     TFRTTYPLAADGGDLIQSLGLTGLPPTPASGGIPNFNFTDGTFISTSPGRPRTISNRT
                     IQLADNLSWVKGRHTLKFGADYQYLEFSDILTFFNGDEFGEYNFTGQFTGNAFGDFLL
                     GLPASTTYAQNGPDGNPFTHNLSFFVQDEIRMTPKLTVNLGLRYELHLPFKDKTNQLA
                     NFDRFFPGGRVVVQNRTGLGLVAQSFKDAIGNTPIVVSSEAGFPEALRETDKNNFNPR
                     IGLAYRPFDNTKTVVRGGFGVYTVTVLGSVLYSMLGVATSNAPVFSN"
BASE COUNT          409 a          416 c          463 g          378 t
ORIGIN      
        1 gtttgaaaac accggcgcat tgctcgtcgc caccccaagc atcgaataca gcaccgaacc
       61 aagcaccgtg accgtgtaga caccaaaccc accccgaacc accgttttgg tattgtcaaa
      121 tggacggtag gccagtccaa tccgtggatt gaagttgttt ttatcggttt cacgaagtgc
      181 ttctgggaaa ccggcttcgc ttgagaccac aatcggcgtg ttgccaatcg catctttgaa
      241 cgattgcgcc accagaccca gaccggttcg attctggacc acgacccgtc cgccaggaaa
      301 aaagcggtca aagttggcaa gctggttggt tttgtcttta aacggaaggt gcaactcata
      361 ccggagcccc agattgaccg tcagctttgg cgtcattctg atttcatcct gcacgaaaaa
      421 ggacagattg tgggtaaaag gatttccgtc gggcccgttc tgggcatagg tggttgacgc
      481 cggcagccca agcagaaaat caccaaaggc attgcccgta aattgtccgg tgaagttgta
      541 ttcaccaaat tcatcgccat tgaaaaaggt taggatgtct gaaaattcaa ggtactggta
      601 atccgcgcca aatttcagcg tgtggcgacc tttgacccac gaaagattat cggcaagctg
      661 gatggtccga tttgaaatcg tgcgcggacg gccagggctg gtcgaaataa aggtgccgtc
      721 ggtgaagttg aaatttggaa tgcctcctga agccggtgtc ggtggaagtc cagtcagacc
      781 aagggattga atcaggtctc cgccatcagc cgccagcggg taggtcgttc gaaaggttga
      841 ccgcgacaga ccggctcgaa attcattgag cactttgggc gtgaaaatcc agttgaagga
      901 tccggccagg ttccggactt cggtgatgcg tgaaaacgtc ccgttttgaa ggttgtagct
      961 tcccgtgccg tccgtgccgg tgtcagtgac atttttatgg ctgtagcggg taaacaccgt
     1021 tttgcgttcg ctgaaggcat ggtcaccgcg caggtcaaag ccattcacgc cgtacgcacc
     1081 gggaacgttg gccacaaaat tgggctggat ggcagctccc gtcgcctgat tttgacgcgg
     1141 atagagcaat tccaaaatcc tggctgacgt cgggttgacc ggaacctgat tgcgggcaaa
     1201 gggttggccc gaaaaaggat taaggagcgg tgtggaaatg ctcgacaggt cgccggctcg
     1261 aaaggcatct ggcggtacga tttctgaaag taaggtctga tttggacgcc gaacaccctc
     1321 atagttgaag aagaaaaatg agcgattgcg tccatcataa aaatttggaa ttgaaatcgg
     1381 cccgctcaga tttccgccga aactattggc caccagcgcc ggtttaagtc gtttcccagg
     1441 ccgcgccggg tcactcggag caaacgggtc agtggcattc aacgcccgat tttgatgaaa
     1501 ccagaaggcg gaaccgtgaa attcgttttg tccgcttcgc gacgtggtgg tgagatcggt
     1561 cacttgccca aattctgcgc tgttgttaaa cgaactgact ttaaactcgg caatgctttc
     1621 aaccgatgga aacaaatccc tgaccggacc attaaaccgg ctgttg
//
LOCUS       DAOQDO010001655         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79553_length_1665_cov_3.537267, whole genome shotgun sequence.
ACCESSION   DAOQDO010001655 DAOQDO010000000
VERSION     DAOQDO010001655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79553_length_1665_cov_3.537267"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1537
                     /locus_tag="PKZ53_21540"
     CDS             296..1537
                     /locus_tag="PKZ53_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43081.1"
                     /translation="MFRFVFIYFFIYNLRFLLSDLPFVGRLGESYENALHIIIPWVGK
                     YVLRLPYDITVFSAGSGDTTYDYVLVLCLIVVSASLALGWTLRKRQQVDDSKLHQWLR
                     VYVRYSLALAMISYGVVKFIPGGQFPAPTLDRLLQPFGDASPMGLLWTFMGASKGYNV
                     FTGAAEIIGGLLLITPRTTLLGSLVCFGVLTNVVMLNLCYDVPVKLYSSHLAAMSIFL
                     MVPDLGRLANLLLFNRPVEPVEYRPLFAQKRLNVAAQLFGVGCMLLFTGWSLYDLHTE
                     LKAETGPEAQSPLRGIWVVEEFEVDGQVRPPLLTEGERWRRVVVDYPGWIGIQLVNDS
                     RQRFLLDLDLEKKTLTLGNRDNPDWEAVLTFDRPESEVLLMEGDLDNHHLRARLHRIP
                     EPQFLLKNRGFHWITEHTFNR"
BASE COUNT          378 a          421 c          428 g          438 t
ORIGIN      
        1 taatggtgga gcaatcattc cgcctgccac cacttcctgg atttattttt atccatcgaa
       61 ctgaagaaat gatgcccagc attcggagcc tgacagattc gacctttgtg gtaaagacgg
      121 tgacgctcta aagcggcgtc aagccgccgc actccgaaag aatccaggtt gcgctgcgct
      181 gcgtagcggg acgtgtcaat ccgaccttcc cagccaactc tgaatttgag ccagcatccg
      241 caaaaaccac agattcatga ataccccctt cgcatcacat ttccttgggc gaattgtgtt
      301 cagattcgtc tttatctatt ttttcatcta taacttacgc ttcttattaa gtgatcttcc
      361 gttcgttggg cgacttggag aatcgtatga aaacgcactc catatcataa ttccctgggt
      421 tggaaagtat gttctgcgat tgccgtacga catcactgtg ttttcagcag gcagcggaga
      481 cacaacctat gattatgttt tggtgttgtg cctgattgtc gtttcagcaa gcctggcact
      541 gggctggacg ctacgtaaac gccaacaggt tgacgattca aaactccatc agtggctgcg
      601 ggtgtatgtg cgctattcgc tcgccctggc gatgatttct tatggggttg tcaagttcat
      661 tcccggaggc cagttccctg ccccaacact tgaccgactg ttgcaaccgt tcggagacgc
      721 ctcgccgatg ggattactct ggacatttat gggtgcctcg aagggctaca atgtgttcac
      781 aggtgcggcg gaaatcatcg gcggtctgtt gctcatcacg ccgcgcacaa cgttgctcgg
      841 gtcgctggtc tgtttcggag tgctcaccaa cgtcgtcatg ctcaacctgt gctatgacgt
      901 cccggtgaaa ctgtattcgt cacatctggc ggccatgtct atatttctga tggtgccgga
      961 tctggggcgg ctggcgaatt tgctgctgtt caatcgtccg gtggagccag ttgaatatcg
     1021 gccactcttt gctcagaagc gattgaatgt tgcggcacag ctctttggtg tggggtgtat
     1081 gctgctcttt acaggctggt cgctgtacga tttgcacacc gaactcaagg ctgaaaccgg
     1141 gcctgaagct caatcaccac ttcgtggaat ttgggttgtt gaagaatttg aagttgatgg
     1201 gcaggttcgt ccgccgctcc tgaccgaggg cgaacgctgg cggcgggttg tggttgacta
     1261 tccgggatgg attggcatcc agttggtgaa tgattcacgc cagcgttttt tactggacct
     1321 tgatctggag aagaaaaccc tcacgctcgg caaccgtgac aatccggact gggaagccgt
     1381 gctgactttt gaccgcccgg aatcagaagt ccttctgatg gaaggggatt tggacaacca
     1441 tcacctccgc gcccgattac accgcatccc agagccacaa tttttgctca aaaatcgcgg
     1501 cttccactgg atcactgaac ataccttcaa ccgctaaagc aaactggcaa aaggagatct
     1561 ccttttgcca actctcaatg cactgactga acttaaagag atgatgaatt gacaaattcg
     1621 aaaatcaaca agaagcaatc aaagttggat gagaaattgg attgg
//
LOCUS       DAOQDO010001656         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79561_length_1665_cov_3.186335, whole genome shotgun sequence.
ACCESSION   DAOQDO010001656 DAOQDO010000000
VERSION     DAOQDO010001656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79561_length_1665_cov_3.186335"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1578
                     /locus_tag="PKZ53_21545"
     CDS             121..1578
                     /locus_tag="PKZ53_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43082.1"
                     /translation="MKTVGLRINCLVYLVMLMALAGFETANAQTVSTFVSGLSEPNDV
                     AFDSTGNMYVAAMRSNKILKVTPAGGVSTFAGSGTYGSQDGPGATAQFAFPTHVTVDS
                     TGNVYVLDSDPYYPIRKITRDGTVSTLKPAIRILSTPPRISNTLVVDPAGNLFFFWAR
                     ELYKFTPTGQTTIVAGSFSNSNIVDGVGTSAYFRNPTGLAIDSQGNLYTLDFNEASGT
                     RFVRKITPNYTVSSIALSEPRQLVDVAVDSTGNLYITGGSVSKNIVKVNNVSAVSIHA
                     GTGAAGATDGPASSATFASPCGAAIFGNFLYVADSGNGKIRKVDVTLLTRKNVALASN
                     GGVASASSTGNETSVPSSATINGEQAGTNWAKGSGGWQDATKDVYPDWVQIDFNGLKS
                     IEEINVFTTQTNYVLNPVTPTPTMTTSSGIKDFEVQYWNGSSWVTVPNGTVTGNTLVW
                     RKFTFSAIPTSKIRVQINNAPGSSRITEIEAWGLN"
BASE COUNT          442 a          374 c          430 g          419 t
ORIGIN      
        1 tcctatttcg atcaggcaac gtcaacactc agtgaaaaat actagagggt agtttgtcaa
       61 aacgaacaag ctatttcatt gagtgggaat agaaaaactt tagctcagga gaaaataaaa
      121 atgaaaacag ttggtttacg aatcaactgt ttggtttatc tggtgatgct gatggctttg
      181 gcgggatttg aaacggccaa tgctcaaacg gtcagtacct ttgtcagtgg gctctctgag
      241 ccaaacgatg ttgcttttga ctcgacaggt aatatgtatg tggcagcgat gaggagtaat
      301 aagattttaa aagtgactcc ggctgggggt gtttctacgt ttgccggtag cgggacttat
      361 ggctcccaag acggtcctgg ggcaacggca caatttgcat ttcctaccca cgtgacagtt
      421 gactcaactg ggaatgtgta tgttttagac agcgatccat attatcccat tcggaagata
      481 acccgggatg ggactgtttc caccttaaaa ccggctataa gaattctttc cactccgccc
      541 cgcattagta atacgttggt tgttgatcct gcgggaaact tgttcttctt ctgggcaagg
      601 gagctttaca aatttacacc cactggacag accacaatcg tggcggggtc attttctaat
      661 tcaaacattg tagacggtgt cgggacctca gcctactttc gtaacccgac gggactggcc
      721 atagactcgc agggaaatct ctataccctc gactttaatg aagcaagtgg gacacgcttc
      781 gtacgaaaga taaccccgaa ctataccgtg agctcaatcg cattgtcgga accgcgtcaa
      841 ttagttgatg tagcggttga tagcacggga aatctctata taaccggtgg aagcgtttcg
      901 aagaatattg tgaaagtgaa taatgtgagc gcggtgagta tccatgccgg aacgggtgct
      961 gctggggcaa ctgatggacc tgcttccagc gcaacgtttg cgtctccctg tggtgctgca
     1021 atcttcggta acttcctcta tgtggcagat tccgggaacg gcaagattcg aaaggtagac
     1081 gtcacattgc tcacccgaaa gaacgtggcc ctggcttcca atggaggcgt cgcgagtgct
     1141 tcatcaacgg gcaatgaaac gagcgtcccg tcctccgcga ccattaatgg agaacaagcc
     1201 ggaacgaatt gggccaaggg gagtggtggt tggcaggatg ccaccaaaga cgtgtatccc
     1261 gactgggtcc agattgattt caatggcctg aagtccattg aggaaatcaa tgttttcacg
     1321 acccagacga attatgttct taatccggtt accccgacgc caacgatgac gacttcaagc
     1381 gggataaaag attttgaggt tcagtattgg aatggctcaa gttgggtaac ggttccaaac
     1441 ggcactgtca ccggcaacac cctggtctgg agaaaattca ccttttcagc gatcccaacc
     1501 tccaaaattc gtgtccaaat caataatgcg ccaggtagca gccgaattac cgagatcgaa
     1561 gcctgggggt tgaattaatg agggttcagg gtttaattcg aagccgtcga actggctggt
     1621 gacttattga agctcaatct gatggtctga acctgcaatc aatca
//
LOCUS       DAOQDO010001657         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79661_length_1664_cov_3.421380, whole genome shotgun sequence.
ACCESSION   DAOQDO010001657 DAOQDO010000000
VERSION     DAOQDO010001657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79661_length_1664_cov_3.421380"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PKZ53_21550"
     CDS             complement(<1..1350)
                     /locus_tag="PKZ53_21550"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNJ43083.1"
                     /translation="MSKLFRLLLCVVLVAGISGMWLRHGSASTSTTTDKQAAGLEIPT
                     TEAMDLWQDVDVATLRAVQQQEVNWINPVEYRSVALNELSLAGILNRAPMEFSAESMN
                     SRVVMTFPMPDGTNQRFAVVESPIMGPELAAEWSHIKTYAGQGIDDPTATIRFDVSPV
                     GLRGMILSAEDTVYIDPAKRGGTNLYMTYSKSKYVRDDKTFDCQMTSPELGDLDQHDH
                     KFSVTPNTLPTENPIRLRTYRLVVCGNAEYGNAAGGGVLANAQAAVVTTVNRVSSLYE
                     RDMAVRLSLVKSYVFVGSTTADPFTNSANTNATTSWSENTTYINNNWGAANYDVGHFF
                     STGAGGAGDIGQVCVAGRKANGATGQSAPTGDGYDIDYVAHELGHQFGGRHTFNSGGT
                     NGCNAGARDTTGPLPNAVETGSGSTVMAYASICGSQDLQLIGQGEGASGQVLAGASDD
                     "
BASE COUNT          374 a          422 c          445 g          423 t
ORIGIN      
        1 atcatcgctc gcgccagcca aaacctggcc gctcgcgcct tcgccctgac caatcaattg
       61 caaatcctgg ctgccgcaga tgctggcata ggccattacg gtgctgccgc ttccggtttc
      121 aaccgcattt ggcagtgggc ccgtggtgtc acgcgctccg gcgttacacc cattggtgcc
      181 gcctgagtta aaggtgtggc gtccgccaaa ctgatggccc agttcgtgag ccacatagtc
      241 aatgtcataa ccatcgccag ttggggctga ttggccggtt gcaccgttgg ctttacgtcc
      301 agccacacac acctggccga tgtcgcctgc accgcccgcg ccggttgaga agaagtggcc
      361 gacatcgtag ttcgccgcgc cccagttgtt gttgatgtag gtggtgtttt cactccacga
      421 agttgtggcg ttggtgttgg cactattggt aaatggatcg gcggtggtgc tgccgacaaa
      481 cacatacgat ttcaccaggc tcagacgcac agccatatct cgttcataca aactggaaac
      541 gcggttgacc gtggtgacca cggcagcctg ggcgttggcg agcactccgc caccagccgc
      601 attgccgtac tcggcgttgc cgcacaccac cagacggtac gtgcgcagac gaatcgggtt
      661 ttcggttggc agtgtatttg gagttacact gaatttgtgg tcatgctgat ccaggtcacc
      721 cagttccggt gatgtcatct ggcaatcaaa cgttttgtcg tcgcgaacat acttgctctt
      781 ggaataggtc atgtagaggt tcgttccgcc acgtttggcc ggatcgatat acaccgtatc
      841 ttcggcggac aggatcattc cacgcagacc gactggagac acatcaaatc ggatggttgc
      901 ggttgggtca tcaatgcctt gtccggcata ggttttgatg tgtgaccatt cggcggccag
      961 ttccggcccc ataatcggtg attcgaccac cgcaaaccgc tgattggtgc catctggcat
     1021 cgggaaggtc atcacgaccc ggctattcat tgattcagca ctgaattcca tcggtgctcg
     1081 attcaaaatt ccagccagcg ataattcatt cagcgcaact gaacgatatt ccaccgggtt
     1141 gatccagttg acttcctgct gctggacggc gcgcaacgta gcgacatcaa cgtcctgcca
     1201 gaggtccatg gcttcagtgg ttgggatttc caagccggcg gcttgcttgt cagtggtggt
     1261 tgacgttgat gcgcttccgt ggcgcaacca catcccactg attccggcaa caagaacgac
     1321 acataacaaa aggcgaaaca atttgctcat aacataacct cagcttggct gaatgaattt
     1381 ttatgtaggt ttgcagtgat tgaaaaaaat gaggataact tgtctttgtg caattctcgt
     1441 gccagatgag gttactttga aagatttacc gtggttctgt aagtgactga aatgaatgta
     1501 tttaagcgat caatagaatc tctgaaggac cagcaaattt ctggcggata aattagtaag
     1561 tgtgtagctg gtatgcagat agtagacgga gtgattgctt ttttattcgc taagttattt
     1621 ggaagcaatg aattctattg aattgtcaag aagaacgatg actg
//
LOCUS       DAOQDO010001658         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79677_length_1664_cov_2.972654, whole genome shotgun sequence.
ACCESSION   DAOQDO010001658 DAOQDO010000000
VERSION     DAOQDO010001658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79677_length_1664_cov_2.972654"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKZ53_21555"
     CDS             <1..609
                     /locus_tag="PKZ53_21555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ43084.1"
                     /translation="TDVMVASERNIDKILDLIQEILEFSRIESQNLSLDTEPVNIEGI
                     LRDCVVTTSVTALQNHITVEADIQENLPLVFGDRVKLERVFSNLLSNAVKFTPTSGKI
                     TIEARQMNQPDSTSQDLPLIAVHISDTGEGIPAEEVPYVFEPYRQATKRKGRLGVGLG
                     LAIVKRIVTAHKGQLCVESQLGVGSRFTVLLPALSAEQLKSE"
     gene            707..>1664
                     /locus_tag="PKZ53_21560"
     CDS             707..>1664
                     /locus_tag="PKZ53_21560"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ43085.1"
                     /translation="MSSAPTSPRTHFRTWVLAFFLPLAIAALSIYHITTPPAVLPATA
                     PPTEFSAERAMTHLWAIAQRPHPSGSIEHQKVREYLVARLKELGLQTEVQKTTALVDR
                     WETAGTVHNILARFPGTNPTKAVMLAAHYDSVPTGPGASDDGAGVAGILEAIRALKSA
                     GPLRNDVIVLFTDSEELGLLGASAFVDEHPWAKDVGVVFNFEARGNRGCSLLFETSEQ
                     NGWLMAEVAKAGAYTIGNSLFYEVYRRLPNDSDMTMFKRGGMIGCNFAFSQGILFYHS
                     ALDHPAYIEQRSLQHHGGYMLNLTRHFGRLDLSNPPRQANVVY"
BASE COUNT          391 a          465 c          418 g          390 t
ORIGIN      
        1 accgacgtca tggtggcatc ggagcgcaac atagataaaa ttcttgacct gattcaggaa
       61 attctggagt tttcacgaat tgaatcccag aacctgagtt tggatacaga acctgtcaat
      121 attgaaggca ttctgaggga ttgtgtggtg accaccagcg tgacagcact ccaaaatcac
      181 attaccgttg aagctgatat tcaggaaaac ctgccgctgg tcttcggaga ccgtgtcaaa
      241 ctggaacgtg tcttttcaaa cctgctctcc aatgcggtta aattcacccc aaccagtgga
      301 aaaatcacca tcgaagcccg gcaaatgaac cagcccgatt caacctcaca ggatctcccg
      361 ttgattgccg ttcatatcag cgatacgggc gaaggaattc cagccgaaga agttccctac
      421 gtctttgaac cttaccgcca ggcgaccaaa cgcaagggac gtctcggcgt tgggctggga
      481 ttagccatcg tcaaacggat tgtcacggcc cacaaaggtc aattgtgcgt tgaaagtcag
      541 cttggggttg ggagccggtt cacggttttg cttccagcgc tttctgctga gcaattgaag
      601 agcgaatagc aaccggcaaa tagtcaatcc acatccaggg cagatcgcaa aagcacgctg
      661 tccccctgtc agtttgtcac cctgtcaccc tgtcaccgat ttcatcatgt cgtctgcccc
      721 cacttcacct cgcacgcatt ttcgcacctg ggtgctggcc tttttccttc cactggctat
      781 tgccgcgctc agtatttacc acatcaccac accaccagcc gttcttccgg caacggcacc
      841 gccaactgaa ttttcagccg agcgtgccat gacccatctc tgggccattg cccagcgtcc
      901 gcatccatcg gggtctatcg aacaccaaaa agtccgtgaa tatctggttg cccggctcaa
      961 agaactcggg ttgcagaccg aagtccagaa aacaaccgcg ctggttgatc gctgggagac
     1021 ggctggtaca gttcacaaca ttctggcccg attccctggt accaatccga caaaggccgt
     1081 catgctggca gcacattatg attcagttcc aaccggaccg ggggccagcg atgacggcgc
     1141 cggcgtggca gggatccttg aagccattcg tgctttaaaa agcgctggcc cgctccgcaa
     1201 tgatgtgatt gtgctgttca ctgattctga agaacttggc ctgctcgggg catcagcttt
     1261 tgtggatgaa cacccctggg ccaaagacgt gggcgtggtg tttaacttcg aagcgcgtgg
     1321 aaatcgtgga tgctccctgc tgtttgagac cagtgaacaa aacggctggc ttatggcaga
     1381 agtcgccaaa gctggggcct ataccattgg aaactctttg ttttatgaag tgtaccgccg
     1441 cctgccaaat gacagtgata tgacgatgtt taaacggggc ggcatgatcg gctgcaattt
     1501 tgctttttca cagggaatcc tgttttacca ttcggcgctt gatcacccgg cctacattga
     1561 gcaacgcagc ctgcagcacc acggcgggta tatgctgaac ctcacccggc attttggccg
     1621 gcttgacctg agtaaccctc cacgccaggc aaatgtggtt tatt
//
LOCUS       DAOQDO010001659         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79698_length_1664_cov_2.396520, whole genome shotgun sequence.
ACCESSION   DAOQDO010001659 DAOQDO010000000
VERSION     DAOQDO010001659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79698_length_1664_cov_2.396520"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKZ53_21565"
     CDS             <1..198
                     /locus_tag="PKZ53_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43086.1"
                     /translation="MSTNDRFVMNNVPLEYWAVIQRLGHRCKVFNYENSKPIFDDFQF
                     TGLSNFDFLTTEFYLGGFDQE"
     gene            204..896
                     /locus_tag="PKZ53_21570"
     CDS             204..896
                     /locus_tag="PKZ53_21570"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNJ43087.1"
                     /translation="MAIFVEGQTERIFVSKLVREIAGEKKVFIATEKPMKSRKGERIF
                     TVIDVTKIPTDEKYYVLIRDCGGDSCVMSDIKDYCEKLSKSGYEKIVGLRDVYPIPAS
                     DIPKLRKGLVYGIPTRYIPIHLVLAVMEIEAWFLAETTHFRRIHDSLTPQVIASTLGF
                     DPTVEDVESRPHPSEDLKTVYKVAGLSYKKRKNSVERTVEAIDYGELYLEQAERVKSL
                     GEFVDQIDTFLT"
     gene            1066..1479
                     /locus_tag="PKZ53_21575"
     CDS             1066..1479
                     /locus_tag="PKZ53_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017110680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ43088.1"
                     /translation="MKQYLGGVSLLVRDYDEAIAYYTGVLGFQLIEDSGRGDGKRWVV
                     VAPPGAQETRLVLAQAKNPAELAHVGHQTGGRVFLFLNTDDFWRDYEIYRARGVKFHE
                     QPRTEIYGTVAVFEDLYGNKWDLLQLTALDTTAEY"
BASE COUNT          470 a          335 c          392 g          467 t
ORIGIN      
        1 atgtctacca atgatcgctt tgtgatgaac aatgtgccac ttgaatattg ggctgtgatc
       61 caacgactgg gtcaccgatg caaagttttc aattatgaaa actcaaaacc aatctttgac
      121 gattttcaat tcactgggct ctccaatttt gattttctta cgactgaatt ttatcttggg
      181 ggctttgacc aggaatgaaa aaattggcga tctttgttga aggtcaaaca gaacgaatct
      241 ttgtctcaaa gcttgtccgt gaaatcgcag gtgagaaaaa agtcttcatt gcaacggaaa
      301 aaccgatgaa aagtcgcaaa ggagagcgaa tattcaccgt tattgacgtt accaaaatcc
      361 caaccgatga gaaatattat gttcttatca gagactgtgg aggcgatagc tgtgtaatgt
      421 ctgatattaa agactattgt gaaaagctat caaagtcagg gtatgaaaag atagttgggc
      481 ttagggatgt ttatcccata cctgcatctg acattccaaa gctaagaaaa gggctggtgt
      541 atggaattcc aacacggtat atcccgattc atttagtgct cgcggttatg gaaatcgaag
      601 catggttctt agcagaaaca actcactttc ggcggattca cgatagtctc acgcctcagg
      661 tgattgcttc aacactggga ttcgacccca ctgttgaaga tgtggaaagt agacctcatc
      721 catccgagga cttgaaaact gtctataaag ttgccgggct cagttataaa aaaagaaaga
      781 actcagtgga aaggacggtc gaagcgattg attatggcga attatatcta gagcaagccg
      841 aaagggttaa aagcttggga gagttcgttg accagattga tacttttttg acctgaatat
      901 ttaattcaga taccgatttc attaagatag cctgtatggc gtaacctgtg gggttaattt
      961 cggattagaa aacttcataa gtcatttgtt tatattggtt acctgtaaaa ttattatcgt
     1021 gtatgtgtaa ttgcacaata ttcgttttct ctgaggatga ttagaatgaa acagtatctt
     1081 ggcggtgtga gccttctggt tcgagattat gacgaggcga ttgcctatta cactggcgtg
     1141 cttgggtttc aactgattga agacagtggc cggggtgatg gaaaacggtg ggtcgttgtc
     1201 gcacctcctg gcgcgcagga aacccggctg gtgctggctc aggcgaaaaa cccggcggaa
     1261 ctggcccacg tcggccatca aaccggcgga cgtgtcttcc tgtttctcaa tacagatgac
     1321 ttctggcgcg actatgaaat ctatcgggcc cgtggcgtca aatttcacga acaaccccga
     1381 accgaaatct atggcacggt ggcggttttc gaggatttat acggtaacaa gtgggattta
     1441 ttgcagttga ctgctctaga cacaaccgct gaatattgat tgacaccaac tggtcaattc
     1501 ctgtaccaga gtgtggtatc aacccagttt cccactggct atttcatgtt caaatcatca
     1561 agccattcct atgacatcca ccaacctcat gatcctgaaa aagaattacc cggtgttttt
     1621 agcagtgctc gtcaccttca tcagtcttgt ggaggtatgg acca
//
LOCUS       DAOQDO010001660         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79701_length_1664_cov_2.338098, whole genome shotgun sequence.
ACCESSION   DAOQDO010001660 DAOQDO010000000
VERSION     DAOQDO010001660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79701_length_1664_cov_2.338098"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKZ53_21580"
     CDS             complement(<1..469)
                     /locus_tag="PKZ53_21580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNJ43089.1"
                     /translation="MPVKHRFFASPEHVLGSTVALSEEESHHAIHVIRLRTGDEVSVF
                     DGCGQEWTATVVMIGRKQVTLELIEKLDDNRESPLELTLAAAQIKADRFEWLLQKAVE
                     LGVSRVIPLITTHTEHGVIKQDSPNRRARWARIVLEATKQCGRRQLMELTEPQS"
     gene            496..>1664
                     /locus_tag="PKZ53_21585"
     CDS             496..>1664
                     /locus_tag="PKZ53_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43090.1"
                     /translation="MSEYQYYEFLAVDRQLTKAEMAELRAISSRAEITSRSLVNEYSF
                     GSFKGDYRALLTKYFDAHVYVANWGTHRFAVKVPKAVIDLKAINQYLHKYSFEMTETP
                     ADVIVEFNSELEEGEGWVEVQPWMSSMIGIRDELLKGDLRSLYLGWLTWVCNDYAHLL
                     DSDSDEDDDDSDDEYEEDDEYEEDDEYEEDDEYEEDGEYEEGDESSVDEGEGSDELEP
                     PVPPGMGELTAAQQSLAEFLRIPRDLLTAVAEASPALKADHDSSTRLAEWITKLPADQ
                     KNAFLLELVQADPGMVRVKLMSQFRAGELSNSKAEKGGARRRSITQLMERANHLTRVR
                     LEAAARKKAEAAARKKAEAERRREVYLAALANTQEAAWKKVDSLIELRMAKSYAE"
BASE COUNT          442 a          380 c          457 g          385 t
ORIGIN      
        1 atgattgagg ttctgtcagt tccatcagtt ggcgccggcc acattgcttg gtggcttcaa
       61 gcacaatgcg tgcccagcgt gcccgccgat tgggtgaatc ctgtttgatc acgccgtgct
      121 cagtgtgggt cgtgataagc ggaattaccc gactgactcc gagttcaacg gctttttgga
      181 gcagccattc aaaccgatca gctttgattt gggcggcggc gagggttaat tcaagcggcg
      241 attcgcggtt gtcgtcgagt ttttcaatca gttcaagtgt cacctgcttg cgtccaatca
      301 ttaccaccgt cgctgtccat tcctggccac atccgtcaaa aactgagact tcatcacctg
      361 ttcgaaggcg aataacatgg attgcgtggt gcgattcctc ttcggaaagc gccacggtcg
      421 agcccagaac gtgctcaggc gatgcaaaaa agcgatgttt tacaggcata gttcaaaagg
      481 agattcagaa atcttatgag tgaatatcaa tattatgagt ttcttgcggt ggatcgtcag
      541 ttgaccaaag ccgagatggc agaattacgt gcgatttcat cccgcgcaga aattacatca
      601 cgatccctgg tgaatgaata cagcttcggc agttttaagg gagattatag agcgttattg
      661 accaaatatt tcgatgccca tgtatatgtt gccaactggg gaacccaccg ttttgcggtc
      721 aaagtaccca aagcggtgat tgacctgaag gcaatcaatc aatatctcca taagtacagc
      781 tttgaaatga ctgaaacccc agccgatgtc attgttgaat ttaacagtga actggaggaa
      841 ggcgaagggt gggtggaagt ccaaccctgg atgtcgtcaa tgattggcat tcgtgacgaa
      901 ttgctcaagg gtgatctccg ctcattgtac ctgggctggt tgacctgggt gtgcaatgac
      961 tatgcgcatt tgcttgatag tgactcggat gaggatgatg acgactcaga cgatgaatat
     1021 gaagaagatg atgaatatga agaagatgat gaatatgaag aagacgatga atatgaagaa
     1081 gacggtgaat atgaagaggg cgatgaatca tcggttgatg aaggggaggg aagtgatgaa
     1141 ctcgaacctc ctgttccacc gggaatgggg gaattgacgg cagcccagca gagcctggct
     1201 gaatttttgc gaattccccg cgatttgttg accgccgttg ctgaagcaag cccggcgcta
     1261 aaagcggatc acgattcgag cacccgactg gcggaatgga ttacaaaact cccggcggac
     1321 caaaaaaatg ccttcctgct cgaactggtc caggctgacc ctgggatggt tcgagttaaa
     1381 ctcatgagcc agtttcgtgc cggtgagctt tcaaattcca aagccgaaaa aggcggagca
     1441 cgccgacgtt ccatcaccca gcttatggag cgtgccaatc atcttactcg ggtcagactt
     1501 gaagcagcgg cccgaaagaa agccgaagca gcggcccgaa agaaagccga agccgaacgc
     1561 cgacgagagg tgtacctcgc tgcactcgcc aacacccagg aagccgcctg gaaaaaggtt
     1621 gattcgctga ttgaattgcg catggcgaaa agttatgccg aaat
//
LOCUS       DAOQDO010001661         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79916_length_1662_cov_2.204729, whole genome shotgun sequence.
ACCESSION   DAOQDO010001661 DAOQDO010000000
VERSION     DAOQDO010001661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79916_length_1662_cov_2.204729"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="PKZ53_21590"
     CDS             complement(<1..1595)
                     /locus_tag="PKZ53_21590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNJ43091.1"
                     /translation="MVSSLGKGIAASSIGCLLEARGLRVTQQKFDPYINVDPGTMSPY
                     QHGEVFVTRDGAETDLDLGHYERFTSAQMTRANNWTTGRVYLSVIEKERRGDFLGKTV
                     QVIPHITNEIKDAILAVSKDVDVTIVEIGGTVGDIESLPFLEATRQLRQQVGRNNAIF
                     VHVTLVPFIASAGELKSKPTQHSVKELLEIGIQPDILLCRTDRELPRDMREKIALFCN
                     VSEDAVVPAMDVASVYEVPLYFAREGLDELIVERLGLEKREPDLRRWQKLVNILKHPD
                     DSVTIGIVGKYVGLGDSYKSLTEALIHGGVANRLRVNIQWVEAEDLRANDNSEEKLRD
                     LDAILVPGGFGKRGISGMIRAISYARKTKTPFFGICLGMQCACIEHARNVCNLPEADS
                     TEFNQESPNPIFFKLRDLIGVEEMGGTMRLGTYPCKLETGSKVAAIYGSELIQERHRH
                     RYEFNVEYEKALGTDENFKFVGKSPDGRFIEMIERADHPWFIGCQFHPEYQSRPLEPH
                     PLFASFVKAAYDRRMREEINEAN"
BASE COUNT          405 a          451 c          410 g          396 t
ORIGIN      
        1 ctgtttgcct cgttgatttc ttcacgcatc cggcggtcat aggcagcttt aacaaacgag
       61 gcaaacagcg gatgcggctc aagtgggcgt gactggtatt ccgggtggaa ctgacagccg
      121 ataaaccacg gatggtcggc gcgttcaatc atttcaataa agcggccatc cggtgatttg
      181 ccgacaaact tgaagttctc gtctgttcca agtgcttttt catattcgac gttgaattca
      241 tagcgatgcc gatgccgctc ttgaatgagt tctgagccgt aaatggcggc gactttgctc
      301 ccggtctcaa gcttgcacgg ataggtgccg aggcgcatcg tgccgcccat ttcctcaacg
      361 ccaatcaaat cgcgcaattt gaagaaaatc ggattgggtg attcctgatt aaactcggtg
      421 ctgtccgcct ctgggagatt gcacacgttc cgggcgtgtt cgatacaggc gcattgcatc
      481 ccaaggcaga ttccaaaaaa cggggttttg gtcttgcggg catatgaaat cgcccgaatc
      541 atgccggaaa tcccacgttt cccaaaccca cccggtacca aaatcgcatc caggtcacgc
      601 aatttttcct ctgagttatc attggcgcgg aggtcttcgg cctcaaccca ctggatattg
      661 actcgcagcc ggttggccac gccgccgtga atcagggctt cggtcaagct cttgtaggaa
      721 tcgcccagac cgacatattt tccaacgata ccaatcgtga cactgtcatc cggatgtttg
      781 agaatattga ccagtttttg ccatcggcgg aggtccggct cgcgtttttc aaggcccagt
      841 cgttccacaa tcaactcatc gagcccttcg cgggcgaaat agagtgggac ttcatagacc
      901 gaggcgacgt ccatcgcggg cacgacggcg tcttccgaca cattgcaaaa caatgcgatt
      961 ttttcacgca tatcacgcgg cagttcgcga tctgtccgac acagcaaaat atcaggctgg
     1021 attccgattt cgagcaattc tttgactgaa tgctgggtcg gtttgctttt taattcgccg
     1081 gcgctggcaa taaagggcac cagggtcacg tggacaaaaa tcgcattgtt gcgaccaacc
     1141 tgctgccgta actggcgggt ggcttccaga aacggaaggg attcaatatc gcccaccgtg
     1201 ccgccaattt cgacaatcgt cacatccaca tcttttgaaa cagcgagaat ggcatcttta
     1261 atctcattgg tgatgtgcgg aatgacctgc acggtttttc ccagaaaatc gccacgccgt
     1321 tctttttcaa tgaccgaaag gtacacgcgc ccggtggtcc agttgttggc ccgcgtcatc
     1381 tgagctgagg taaaccgttc ataatgacca agatccaggt cagtttcggc gccatcacgg
     1441 gtcacaaaga cctcaccgtg ttgatacggc gacatcgttc cagggtcaac attgatatat
     1501 ggatcaaatt tctgttgtgt tacccgcaga ccacgggctt cgagcaagca cccaatggat
     1561 gatgcagcaa tgcctttgcc aagcgatgaa accacaccac ccgtgacaaa aatatactac
     1621 gacacacaaa cctccaatca gaaaacaaaa tgacaagatg cc
//
LOCUS       DAOQDO010001662         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_79987_length_1661_cov_2.837484, whole genome shotgun sequence.
ACCESSION   DAOQDO010001662 DAOQDO010000000
VERSION     DAOQDO010001662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79987_length_1661_cov_2.837484"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PKZ53_21595"
     CDS             <1..1194
                     /locus_tag="PKZ53_21595"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNJ43092.1"
                     /translation="PMKGTAARACTLAGDQAQAQWLFHSEKNRAENIMIVDMIRNDIG
                     RVAHIGSVTVPKLFDIERYPTVWQMTSTVAATTDEPVWKILKALFPCASITGAPKARS
                     MKIIAELESAPRHIYTGSIGYLAPGRKAQFNVAIRTVVLDRRTGQAEFGVGGGIVWDS
                     EQKDEYQECQTKAKVLTSRWDDFQLFESLLWTPEDGYFLWSNHLSRLRDSAAYFGFVV
                     DESRLVAELEQLARHLPRLAHKVKVSVSRRGELSHECLALPVSPAPAMVTVRFARSPV
                     QSTDRFLYHKTTRRDVYTQALAPFPECDDVVLWNERGEVTESTRANVVIRINGKLVTP
                     PVSCGLLAGTFRNRLIEKGELTEQIITREDFEQADARFLINSVRKWQAVELLATVQGK
                     PAAST"
     gene            1345..>1661
                     /locus_tag="PKZ53_21600"
     CDS             1345..>1661
                     /locus_tag="PKZ53_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43093.1"
                     /translation="MNNLLIKALPLMFGIGVCLCGILNSMSVQAQVIPLDIVFTPAIE
                     TAKPEPTERFPGEAEIDALLTFSGKPSVSQAREDAPLPQTSQPSTPSADPGQVKKDPY
                     QVNP"
BASE COUNT          401 a          428 c          446 g          386 t
ORIGIN      
        1 ccaatgaagg gaacggcggc ccgggcctgt actctggctg gtgatcaggc tcaggcacaa
       61 tggttgtttc actctgaaaa aaatcgggcc gaaaacatta tgattgtgga catgatccgc
      121 aatgacatcg ggcgggtcgc gcacattgga agcgtcacgg ttccaaaact ctttgatatc
      181 gaacgctatc cgaccgtctg gcaaatgact tcgacggtgg cggccacgac ggacgaaccc
      241 gtgtggaaga ttctaaaagc gctcttccca tgcgcttcga ttactggggc gccaaaagcg
      301 cgttcaatga agatcattgc cgaactcgaa tctgcccccc ggcatatcta caccggaagc
      361 atcggttatc tggcgccagg gcggaaggca cagttcaacg tggcaattcg aactgtggtg
      421 ctggatcgga gaaccgggca ggccgaattt ggcgtgggcg gcgggattgt gtgggattct
      481 gagcagaaag atgagtacca ggagtgtcag accaaggcga aggtgttgac cagccggtgg
      541 gatgattttc agttgtttga atcactgctc tggacacccg aagatggcta ttttctgtgg
      601 tcaaatcacc tgagtcggtt gcgtgattcc gccgcctatt ttgggtttgt cgtggatgag
      661 tccaggctgg ttgccgaact ggagcagctt gcccgacacc ttccacggct ggcacataaa
      721 gtgaaagtga gcgtttcgcg tcgcggggaa ctgagccacg agtgccttgc tttgccggtt
      781 tcgccagcgc cagccatggt cacagtgcgg tttgcccgat caccagttca atcaaccgac
      841 cgatttctgt atcacaaaac gacccgacgt gacgtctata cccaggcgct ggcgccattt
      901 cccgaatgtg atgatgtcgt cttatggaac gagcgtggcg aagtaactga aagtacaagg
      961 gcaaatgtcg ttatccggat aaacgggaag ctggtgacgc caccggtttc ctgcgggtta
     1021 ctcgcgggaa ccttccggaa ccggttaatc gaaaaaggcg aactcaccga acagatcatt
     1081 acccgtgaag atttcgaaca ggctgacgcc cgctttctga tcaattccgt tcgcaagtgg
     1141 caggcagttg aattgcttgc aacggttcag gggaaaccgg ctgcttcaac ctgaaaggaa
     1201 ccgaccaggt tataattcac tatcaaccca aactcgtgca tttgaaccag taacaggaaa
     1261 ctttcaggaa gatgtatttg cccaaaccga ccttttctcg ctagggttcg cggccaaatc
     1321 tatcttcgct cactttatcc aactatgaac aatctattga tcaaagcttt gcccctgatg
     1381 tttggaattg gagtctgtct gtgtggaatt ctcaactcga tgtctgttca ggctcaggtg
     1441 ataccgcttg atattgtctt taccccggcg attgaaaccg caaaacctga accgacagag
     1501 cgatttccag gagaagcgga aattgatgcg ctgctcactt tttctggcaa accatctgtt
     1561 tctcaagcca gggaagatgc acctctgcct caaacctctc agccgtcaac tccgtcagcc
     1621 gatccaggtc aggtgaaaaa agacccgtat caggtcaacc c
//
LOCUS       DAOQDO010001663         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80044_length_1660_cov_3.391277, whole genome shotgun sequence.
ACCESSION   DAOQDO010001663 DAOQDO010000000
VERSION     DAOQDO010001663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80044_length_1660_cov_3.391277"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..518)
                     /locus_tag="PKZ53_21605"
     CDS             complement(36..518)
                     /locus_tag="PKZ53_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43094.1"
                     /translation="MNNYSAPTSSLPPTDLAPALSGSAVTSRTIAVTGGQGIGKTHFV
                     ANVALELALRRRGITVVNIENGSVTSNILLEAGPAYNLSHVLIGACQLNEIMTATPQG
                     IRLISASRGMSEFGLIGATHQVLTELEQVEQNSFFTRKNARKPRPQPRSGRAGMNRSR
                     "
     gene            complement(775..1620)
                     /locus_tag="PKZ53_21610"
     CDS             complement(775..1620)
                     /locus_tag="PKZ53_21610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNJ43095.1"
                     /translation="MTDRPRRRIIYKKARSHDSHHGGAWKVAYADFVTAMMALFLVLW
                     LVTQGDQKLKENLANYFRSPGVFQTTNGGIFDGSNKALSSPVSLTHSEPASQAALYDT
                     AVILKKQLGAGGKLKSFHDQVKVDMVKQGLRIQILDKAEQSSFESGSATLNPTTRHLL
                     DEIAMVICKLPHKIQIGGHTDAYTYPTSHFTNWELSADRANAARRELEAACIDPKRIH
                     RIVGYADTELLDPEHPYAPSNRRISIILLGNENAHPEVLPLTNEEFGIVMGRPKPGSD
                     GNSKF"
BASE COUNT          382 a          408 c          448 g          422 t
ORIGIN      
        1 attactttgt ttaagatcgg tcagtcagat ttttttcagc ggctgcgatt catccccgcc
       61 cgaccgcttc gcggttgagg acggggcttt ctcgcatttt ttctggtaaa aaacgagttt
      121 tgttcgacct gttccagttc ggtcagaacc tggtgggtgg cgccaatgag tccgaattcc
      181 gacatcccac gacttgcgga aatcagtcgg atgccctgtg gcgtggcggt cataatttcg
      241 tttaactggc aggccccaat caacacatgg ctcagattat aggctgggcc agcttcgagc
      301 aggatgttgg atgtcaccga gccattctca atattgacaa ctgtaattcc tcgccgtcgc
      361 agggccagtt caagggcgac attggcgaca aaatgcgtct ttccaatgcc ttgcccgcct
      421 gtcaccgcaa tggttcggct tgtgaccgca ctcccagaga gtgcaggcgc cagatcggtc
      481 ggcggaagcg aagaggttgg tgcagaataa ttgttcatgg gggcctcgaa caaaagtttg
      541 ggtcgttctt taactggaaa gaatctgagc cgacataatt acaaaagatt gattggcgtc
      601 gccgcacgtc tgggtgctgg ataccggttt gccgaaataa actgaactac aacgtgcaat
      661 tgagatgcca caatccacac tatcctggaa acaccgtggt gcaaaccaac gcctggatca
      721 atcacggcac gccacctggt tatgactgat tctgagttct gacacgaggg aggttcaaaa
      781 cttactgttt ccatcggacc ccggtttggg gcgtcccatc acgatgccaa attcttcatt
      841 ggtaagaggt aaaacttcag gatgggcgtt ttcattgccg agcaggataa tgctgattcg
      901 gcgattggag ggggcgtatg gatgttcggg atccaacagc tcagtatcgg catagccgac
      961 aatgcgatgg attcgctttg gatcaataca ggctgcttct aactcacgcc gggcagcatt
     1021 ggcccgatct gccgaaagct cccagttggt aaagtgtgaa gtcggatagg tataagcgtc
     1081 ggtgtgtccc ccaatttgaa ttttgtgtgg gagtttgcag atcaccatgg caatttcatc
     1141 gagcaggtgc cgggtcgttg gattgagcgt ggcactgcct gattcaaatg aactctgttc
     1201 agccttatcc aaaatctgga tgcggagtcc ctgtttgacc atatccactt tgacctggtc
     1261 atgaaacgac tttaatttcc ccccggcccc aagctgtttt ttgaggataa cggctgtgtc
     1321 atacaacgct gcctgtgagg caggctccga atgcgtgagc gaaaccgggc tggacagggc
     1381 tttgtttgag ccatcaaaaa ttccgccatt ggtggtttga aagacaccag gcgagcgaaa
     1441 gtagttggcc agattttcct tgagcttttg atcgccctgc gtcaccagcc acagcaccaa
     1501 aaacagcgcc atcatggctg tgacaaaatc agcatacgcc actttccagg cgcctccgtg
     1561 gtggctgtca tgagatctgg cttttttata aataatgcgg cggcggggac gatcagtcat
     1621 gagaataccc gtttgcagtc agtcgttgta ccattttggg
//
LOCUS       DAOQDO010001664         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80072_length_1660_cov_2.707165, whole genome shotgun sequence.
ACCESSION   DAOQDO010001664 DAOQDO010000000
VERSION     DAOQDO010001664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80072_length_1660_cov_2.707165"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="PKZ53_21615"
     CDS             complement(<1..1551)
                     /locus_tag="PKZ53_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43096.1"
                     /translation="MYRFTSYSVNTGLPHNSVYCLFQDSQGYMWFGTESGVCRFDGLE
                     YKTFTVRDGLADPVVRDIFEDRQNRLWIVTESGVSRFDGLRFTSFKAAQGFSPEEAYS
                     GMCSRDGTLWFGTADGLIHYDGQTFTRFGKDQGVPTGRIWSLVEDQAGKIWLGIRGEG
                     LLRYDGKTFTRFGVNEGLPNEVIFGLAEDPRGGLWITTDQGLVFYDGFRFRTYSLPEG
                     LPISKTNQVVIDRYYRIWVTTFGGGLCRLEDGKFVVFNRTNGVPDNYLTSITLDYEGN
                     IWCATRSSGVFRFSSEQFASYTQASGLSEGRVSALGETPDETLWVASVDGGLRTMSKS
                     GDVNRLGTPEGLPDKDIWTLYVDRLNRIWASSYQGLFCRENGRWRKFTREEIGARERI
                     TSIIEDHQGNIWLGSYPSTSNGVLRYDGKSFTLFSTDHGLSSNSVSGFMVDHTGSLWA
                     CNERGVSRFNGQRFSSLTPADGLPSRRVSCALEDERGVIWIGTDSGLCQFVNEKIVKI
                     YTTDDGMVG"
BASE COUNT          415 a          493 c          386 g          366 t
ORIGIN      
        1 tccgaccatg ccatcatcgg tggtgtagat tttgacgatt ttttcattca caaactggca
       61 caacccgcta tcggtcccaa tccagatcac accgcgttca tcttcgaggg cgcaactgac
      121 ccgccggctg ggtaatccat ccgctggggt cagcgaagaa aaccgctgcc cgttaaagcg
      181 actgacccca cgctcattac aggcccacag gctgccggtg tgatcaacca taaacccgga
      241 tacagagttg ctgctcaagc cgtggtcagt ggaaaataac gtgaatgact ttccgtcata
      301 gcgcagcact ccgttactgg ttgacgggta ggacccaagc cagatgtttc cctgatggtc
      361 ttcgatgatt gacgtgatgc gctcgcgggc cccgatttct tcgcgggtaa attttcgcca
      421 tcgtccattt tcccgacaaa agagtccctg ataactgctt gcccagattc ggttgagtcg
      481 gtcaacatag agtgtccaga tatctttatc cggaagcccc tctggtgttc ccaggcggtt
      541 gacgtcgccc gatttggaca tcgttcgcaa cccaccgtca accgaggcca cccagagtgt
      601 ttcatccggt gtttctccca gggcactgac ccggccttca ctcagcccgc ttgcctgggt
      661 gtagctggca aattgttcgc tgctaaaccg aaagacccca cttgaacgag tggcgcacca
      721 gatattgcct tcataatcga gggtaattga cgtgagatag ttgtcaggta cgccattggt
      781 acggttaaac accacaaatt tgccgtcttc caggcgacac aagcctccgc caaacgtcgt
      841 cacccaaatc cggtagtagc gatcaatcac gacctggttg gtctttgaaa tgggcaatcc
      901 ttccggcagc gaataggtcc gaaaccgaaa tccatcataa aagaccagtc cttgatcagt
      961 ggtaatccag agtcctccgc gtgggtcttc agccagccca aaaatgactt cattgggcag
     1021 tccttcattg acaccaaacc gcgtaaaggt tttcccatca tagcgaagca acccttcacc
     1081 ccggatccca agccagattt ttcctgcctg atcctcaacc agcgaccaga tgcgacccgt
     1141 cggaacgccc tggtctttgc caaaccgggt aaaggtctgg ccatcataat gaatcagccc
     1201 atcagccgtg ccaaaccaga gcgttccatc acggctgcac attccagaat acgcttcttc
     1261 gggggaaaac ccctgggcag ccttaaacga agtaaaccga agcccatcaa agcggctgac
     1321 accactttcc gtaacaatcc agagtcgatt ttgacgatct tcaaaaatgt cgcgcacaac
     1381 tggatcagct aatccatcac gaacggtaaa tgttttgtat tcaagcccat caaagcggca
     1441 aactccagat tccgttccaa accacatata gccctggctg tcctgaaaca aacaatagac
     1501 gctgttatga ggtaacccgg tattgacgct atagcttgta aatcggtaca cctggccgca
     1561 aacacgtggc tggcccccgg gccacccaag aaacgccatc aggagaccca gccagagaat
     1621 ggactgtttt gcgccgaatc gcgtcaggga ttgccgatca
//
LOCUS       DAOQDO010001665         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80075_length_1660_cov_2.606231, whole genome shotgun sequence.
ACCESSION   DAOQDO010001665 DAOQDO010000000
VERSION     DAOQDO010001665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80075_length_1660_cov_2.606231"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..>1660
                     /locus_tag="PKZ53_21620"
     CDS             83..>1660
                     /locus_tag="PKZ53_21620"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ43097.1"
                     /translation="MQSDPEELRQAARQREAQRARVLLVGRQQELTAIVNLPDALTAD
                     KGQLDTLSEWNQKGNQPLQNGFVRLIPEIVSVEIDRTALKAKTVADGSGTMLRQVQSD
                     ELVTTLKFQIAGAFQIRPCLSNVRLPKGTTIWTYAETGEPQGPVGIDLVTPDGDLWCP
                     TIEGSTVWIEIHIPKAAFSGSVSFSFQLSKALETFRLTQKGVPLVESQHRGVFQPLDD
                     SCLQDVNCVQQTQLISDASRAVGRMQFISGGSGFVCTGGLLNDQDTSGFIPYFLTANH
                     CLSTQSEVSSLEVWWDYKTSTCNGSPPSSNSLQKSFGGTLLATSAGSDFTFIRLQSVP
                     SNRIFLGWNASTLGSGVQLFRVHNPGGRVQHYSEHRTVSPTSTCQSLPPSAFLYSEDL
                     VAATAGGSSGSPVLNSSEQVVGQLFGACGPDPENNCDRRNRTVDGRFSTTFPQIRQWL
                     EGSGNGGDCTPASISGNSFSTTASITNSSCTVNFGTYTLPDTGEALGTSRLSNLYSFS
                     GSSGEKVTITMGSSALDC"
BASE COUNT          376 a          489 c          428 g          367 t
ORIGIN      
        1 gctcacaccc ggccttcagg catcttcgaa ccccgaaccg gttgtcacgt ctcaggaggc
       61 agcacccgct tgcccacccg ccatgcaatc tgacccggag gagctccggc aagccgcccg
      121 gcaacgcgaa gcacagcgcg ccagagtgct gctggttgga cgacagcagg aactcaccgc
      181 cattgtgaac ctgcccgatg ccctcacggc tgacaaaggg caacttgata ccctgagcga
      241 gtggaaccaa aagggcaatc aaccactcca gaacggtttt gtgcgcctga tcccagagat
      301 tgtgtcggtg gaaattgacc gaacggcgct caaagccaaa acggtggcgg atggatcagg
      361 aaccatgctc cgacaggttc agtctgatga actggtgacc acgctgaaat ttcagattgc
      421 cggtgccttt cagattcgtc cctgcctgtc aaacgtccgg ctgcccaaag gtacgacaat
      481 ctggacctac gccgaaactg gcgaaccgca ggggccggtt ggcattgacc tggtaacacc
      541 cgatggcgac ctgtggtgtc cgacgattga agggtcaacc gtctggattg aaattcatat
      601 tcccaaagca gcgttcagcg gttcagtaag cttctctttt caactcagca aagcgctgga
      661 aacgtttcgg ctcactcaaa aaggcgttcc gctggttgaa tcccagcacc gtggggtctt
      721 tcaaccgctg gatgattcct gtctccagga cgtcaattgc gtccagcaaa cccagctcat
      781 tagcgatgcc agccgggcag tcggcagaat gcaatttatc agcggcggaa gcggctttgt
      841 ttgcaccggc ggtttgctca atgaccagga cacaagcggg tttatcccct actttctgac
      901 cgccaatcac tgtctttcaa cccaaagcga agtttcctcc ctcgaagtct ggtgggatta
      961 caaaacttcg acctgcaacg ggtcgccgcc gtcatccaat tcattacaaa agagttttgg
     1021 tggaacgttg cttgcaacca gtgcgggcag tgattttaca tttattcgcc tgcaaagcgt
     1081 gcccagcaat cgaatttttc tcggctggaa cgccagcacc cttggctcag gcgttcagct
     1141 cttccgggtg cataaccccg gtggtcgtgt ccagcactat tccgaacacc gcaccgtcag
     1201 cccgaccagt acctgccaat cgctcccgcc cagtgccttt ctctattctg aagatctggt
     1261 cgccgccacc gctgggggga gttccggttc gccagtcttg aatagctcgg aacaggtcgt
     1321 cggtcaattg tttggcgcct gcggccccga ccctgaaaat aactgtgacc gccgcaatcg
     1381 aaccgtagac ggacgcttta gcacgacctt tcctcaaatt cgccagtggc ttgaaggttc
     1441 cggaaatgga ggcgattgca caccagcttc aatttcaggc aatagttttt ccaccaccgc
     1501 atcaattaca aattcttcct gtacggtaaa ttttggcact tatacgttac ctgataccgg
     1561 cgaagcactc gggaccagcc ggttaagcaa cctgtattca tttagcgggt caagcggtga
     1621 aaaagtcacg atcacgatgg gttcatcggc gctggattgt
//
LOCUS       DAOQDO010001666         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80246_length_1658_cov_3.304429, whole genome shotgun sequence.
ACCESSION   DAOQDO010001666 DAOQDO010000000
VERSION     DAOQDO010001666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80246_length_1658_cov_3.304429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKZ53_21625"
     CDS             <1..633
                     /locus_tag="PKZ53_21625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43098.1"
                     /translation="LEAERARRETKPVIQVIINLIRNAAEATQENKGQIEVALTVEGN
                     DALIAISDDGSGISPENLKHIWEPFFSTKGEEGTGLGLEICRRIIEAHGGTISCESTE
                     NVGTTFTIRLPLNLETQELAADMFDTITDAYPLKKIKAPSVTPTQLAQLPADVLNTLY
                     QAVLEGDIEAANQVIDQILELDSYVAEVVQAMLKEYKFDELQELIEAATM"
     gene            complement(760..1605)
                     /locus_tag="PKZ53_21630"
     CDS             complement(760..1605)
                     /locus_tag="PKZ53_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNJ43099.1"
                     /translation="MKLQLETILGQPVKRLSALSGGCVGEVYRVWLENGNSVVAKVDQ
                     RRNAVLEIEGFMLQYLRDHSSLPVPAVMYSSPNVLIMEYIEGGSTFPMAAERHAAELL
                     AALHTVKAEQFGLEQDTLIGGLHQPNSWTASWIEFFRDQRLLFMGREALQSGQLPRTV
                     FSRLEKFAERLDEWIEEPDHPSLIHGDVWTTNVLSHQGRITGFIDPAIYYGHPEIELA
                     FTTLFHTFGQAFFTRYQELKPIKPGFFEIRRDLYNLYPLLVHVRLFGGGYVGSVSGIL
                     SRIGF"
BASE COUNT          443 a          431 c          425 g          359 t
ORIGIN      
        1 ctggaagcag aacgtgctcg gcgggagacc aagccggtga tccaggtcat catcaacctg
       61 atccgaaatg ccgccgaagc cactcaggaa aataaaggac agatcgaagt tgccctgacc
      121 gttgaaggca atgacgccct gatcgccatt tctgacgacg gaagcgggat ttccccagag
      181 aacctcaagc atatctggga gccgtttttc tcaaccaaag gtgaagaggg caccggactt
      241 ggcctggaaa tctgtcgccg aattatcgaa gcccacggcg gaactatttc gtgtgaaagc
      301 acagaaaacg tcggcaccac gtttaccatc cggctgccgc ttaacctgga aactcaggaa
      361 cttgcagccg atatgtttga cacaattacg gatgcgtacc cgctcaaaaa gatcaaagcc
      421 ccatcagtga cacccaccca actggctcaa ctcccggcag atgtcctcaa caccctctat
      481 caggcagttt tggaaggaga tatcgaagct gccaatcaag tcattgatca aattcttgaa
      541 cttgacagct atgtggctga ggtggttcag gccatgctga aagagtataa gtttgatgaa
      601 ctccaggaac tgatcgaagc tgcgacgatg tgacaggcag ggtgacaagg atgaccgagt
      661 aacaacctga cagggtgaca gaggcagaag gtgacaaagc acgagaggat catcctgacg
      721 actcgcgctc ttgcgcttct gcctgatttc ttcacccgct taaaatccaa ttcgtgacaa
      781 gattccactc accgagccca catatccacc gccaaacaaa cgcacgtgaa ccagtagtgg
      841 atagaggttg tacaaatctc gtcgaatttc gaaaaatcct ggtttgattg gctttagctc
      901 ctggtaccgg gtaaaaaaag cctgaccgaa cgtgtgaaag agtgttgtaa aggccagttc
      961 aatttcagga tgaccataat aaatcgccgg gtcaataaat ccggtgattc gtccctgatg
     1021 gctcagcaca ttggtggtcc agacgtcacc gtgaatcagt gatggatggt caggttcttc
     1081 gatccactca tcgaggcgtt cggcaaattt ctccaggcga gaaaagacag ttcgtggcag
     1141 ttgacctgac tgcaaagctt cccgccccat aaacaacagg cgctgatcac ggaaaaactc
     1201 gatccaggag gcagtccagg agtttggctg atgcagccca ccaatcaagg tatcctgttc
     1261 aaggccaaat tgctctgcct tgacggtgtg aagtgccgcc aggagttcag ccgcgtgacg
     1321 ttcggcagcc atcgggaagg tgctgccgcc ttcgatgtat tccataatca gcacatttgg
     1381 cgacgaatac atcacagccg gcaccggaag tgacgaatgg tcccgcaggt actggagcat
     1441 aaatccttca atctcaagca ccgcgtttcg tcgctgatca accttggcaa ccactgaatt
     1501 tccattttcg agccagactc gataaacctc acccacacag cctccgctga gtgctgacag
     1561 tcgtttgacc ggctgaccaa ggattgtttc aagctgtaat ttcatagttc ggggttcagg
     1621 gttcagggtt cagggggtag ggttcagggt tcagggtt
//
LOCUS       DAOQDO010001667         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80270_length_1658_cov_2.486588, whole genome shotgun sequence.
ACCESSION   DAOQDO010001667 DAOQDO010000000
VERSION     DAOQDO010001667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80270_length_1658_cov_2.486588"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PKZ53_21635"
     CDS             complement(<1..1137)
                     /locus_tag="PKZ53_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43100.1"
                     /translation="MFKPVLSLTVLLGMIASMVPAFAQSPRSSTDSHSDPVISVTEAS
                     SKAPAASQPGAENEATGHPPSSDDRATAVRDPLARLLMTKGLLTLTEAQSVSASDSSP
                     VQREQLTRLLLNKGILSKAEYDTLLRPLPVQTATQGPLTASNNSHAAVSGGVLKTTTP
                     LILPAIAPIRVLQTEPSKREGLIPDVRIGSSIRLKFFGFFKTSAIYDSSDPTGSDFPL
                     PGFLSDSGPTGSPSFHIKARSLRIGANFEWLDPSPKTTITGKLELDFEGNFARASNRN
                     IGSIRSNAPTLRLAYMRIDRKFTENTAGFLLFGQDWTPFGSSTLPNLIETTGHGIGYG
                     TLYDRTPQIKTGFLWKAGGKRNWQLLPEFALTLPAFGNMPANVSD"
BASE COUNT          438 a          390 c          443 g          387 t
ORIGIN      
        1 gtctgaaaca ttggctggca tattgccgaa ggccgggagc gtcaacgcaa attcaggcaa
       61 caattgccag ttccgttttc caccggcctt ccacaaaaaa ccggttttga tttgaggtgt
      121 gcgatcatag agcgttccat agccaatacc gtggccggtg gtttcaatca aatttggaag
      181 ggttgaagaa ccaaacggcg tccagtcctg accaaagagc aaaaacccag cggtgttttc
      241 tgtgaatttg cggtcaatcc gcatataagc cagcctgagt gttggcgcat tggatcgaat
      301 cgaaccaata ttgcggttgc tggcccgggc aaaattgcct tcgaaatcaa gttcgagttt
      361 cccggtaatc gtggttttag gtgacgggtc aagccattca aaattggccc caatccgtaa
      421 actccgggct ttgatatgaa aactcgggga cccggtcggt ccactgtcac tgagaaaccc
      481 tggcaacgga aaatcactcc cggttgggtc agagctatcg taaatggcac tggttttaaa
      541 gaatccaaaa aatttaagtc gaatactcga tccaatccgg acatccggaa tgagtccttc
      601 ccgttttgac ggttcagttt gcaagactcg aatcggagcg atagccggaa ggatcagtgg
      661 cgttgtggtt ttcaagacgc ctcccgaaac agccgcgtgc gaattgttgc tggctgtcag
      721 cggcccctgg gttgcagttt gaaccggaag cggtctcaac agcgtgtcat attccgcttt
      781 tgaaagaata cctttgttca acagcaacct ggtcagttgc tcgcgctgga ccggactact
      841 gtctgaagcc gagaccgact gcgcttcggt gagggttaaa agtcctttgg tcatcagtag
      901 tcgtgccagc gggtcgcgaa ccgcagtcgc tcgatcatct gatgaggggg gatgacccgt
      961 ggcctcgttt tcagcgcccg gctggctggc agcaggcgct ttggaggatg cttcagtgac
     1021 tgaaatgact gggtcagagt gtgaatcggt tgatgaccgg ggagactggg caaaggcagg
     1081 aaccattgag gcgatcattc ctaaaagaac cgtaagcgaa agaacaggtt tgaacataac
     1141 ggcattccga aacttggctg gaatgaggaa cgcggcaaac ggggggattc acattccact
     1201 ctggattgat ggaatcacaa gcttccatcg tgtcactcag gctctgtgca aagtccgtgc
     1261 ccaaatcagt catcagggaa agactatagg aatgtacttt tttatcaatg ttttagatat
     1321 tcttatccaa ccagacacac caatggaaaa attgctcagt ggggaattct tttggggtga
     1381 gttgtgaggg aatttactgg ctcagccaaa aaccgcacgc cgtagcttct gatgtcgaag
     1441 ctacggcgtg cggcgtggaa aagacgttga actggtcaaa tcaacgatga ggatgagttt
     1501 tggcataatc atatgcggta taggctgaaa tcaagtgaca aacccagcca agcataccgc
     1561 ccgtgccaac ccagaatcca ggtgtaatga tcaaccagat aattccccgg aaaaaacaac
     1621 cgttgtaaat ttctccgaca ccaggaatca aaaagctc
//
LOCUS       DAOQDO010001668         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80280_length_1658_cov_2.353088, whole genome shotgun sequence.
ACCESSION   DAOQDO010001668 DAOQDO010000000
VERSION     DAOQDO010001668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80280_length_1658_cov_2.353088"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PKZ53_21640"
     CDS             <1..1264
                     /locus_tag="PKZ53_21640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ43101.1"
                     /translation="AERMCLPEQRISLLHQIVDLSLSLQKFEDAYQRMRTLLEILIRD
                     GKSTTALEAITKLPSMGARDAQYRKELTEYVNLHREEWTQGARGTWIPDESDLPPVDV
                     TSRQLEMKFTTHTVLVVDDEPGILQILSMGIKRLGCRVVTASNGEEALQLAEQFQPNL
                     IISDLVMPQMDGSQLFSSLRKHPTLSHVPFVCLTSNRLEKERLAALNRGVEDFWIKPF
                     SLAEVVLRVGKLFLRHRKTADFSGQLSRVAFPELLQLLEHGGKTGMLKLRSDTREATL
                     FFDQGVMLDARYDSFPSEVVVYKLIHWMSGEFMFFAQPVNCEKKLKFNTQQLLMEGLR
                     RYDEASCLMDELSADVSKVYTFRCDPRRLHFEPEVIPYLHRIEGLFNGCKTLRVCCEA
                     LAGELESLVVVVELIEQQILIPDPSFEQ"
BASE COUNT          436 a          387 c          400 g          435 t
ORIGIN      
        1 agccgagcgg atgtgccttc ctgagcagcg gatctcattg ctgcatcaaa tcgttgacct
       61 gagcctgagc cttcagaaat tcgaagacgc ctaccagcga atgcgtacgt tgctggagat
      121 tctgattcga gacggcaagt caaccacggc cctggaagca atcaccaaac tcccctcaat
      181 gggcgccagg gatgcgcaat accggaaaga actcacagag tatgtcaacc tgcaccgcga
      241 ggaatggact caaggtgcca gagggacctg gattccggat gaatctgatc ttccgccagt
      301 cgatgtgacc agtcggcaac tggagatgaa gttcaccacc cacaccgtgc tggtggttga
      361 cgatgagcct gggattcttc aaatcctgag tatggggata aagcggcttg gatgcagggt
      421 ggtcaccgcc tccaatgggg aagaagcact ccaactggcg gaacagtttc aaccaaattt
      481 gatcatttcc gatctggtga tgccccaaat ggacggcagc cagctttttt catcgcttcg
      541 caagcatccg acgctcagtc acgtgccgtt tgtgtgcctc acctcaaacc gattggaaaa
      601 agaacgcctg gcggcgttaa accgtggagt cgaagatttt tggatcaaac cctttagcct
      661 ggcggaagtg gtcttgcgag tcgggaaact ctttcttcgc caccggaaaa cagccgattt
      721 ttcaggccaa ctgtcccgag tcgcgtttcc cgaattactg caactccttg aacatggagg
      781 gaaaacaggc atgttgaagc tgcgctctga tacgcgtgag gcgaccttgt tttttgacca
      841 gggagtcatg cttgatgccc gatatgatag ttttcccagt gaagtggtcg tgtacaaatt
      901 gatccactgg atgtctgggg aatttatgtt ctttgcccag cccgtcaatt gtgaaaaaaa
      961 acttaaattc aatacccagc aacttttaat ggaagggctg cggcgatatg acgaagccag
     1021 ttgcttaatg gatgagcttt cggctgatgt ctcaaaggta tacacatttc gatgcgaccc
     1081 gcgacgactt cactttgaac cagaagtcat tccctatctt caccggatcg aggggctctt
     1141 caacggatgt aaaaccttgc gggtctgttg cgaagctctg gcgggtgagt tggaatccct
     1201 ggtggtagtg gtggaactga tcgaacaaca aatcctgatt ccagatccta gctttgaaca
     1261 atagttttta ataccagttt gtagtcagta gcccgtagtc agtagttcac caagtctatt
     1321 tgattgaatc acttgactat tttctaatac aaggattcca ttccaaattg gtataacctc
     1381 attatttaca acagtattcg ggaaattccg aaatcaggtt tagcctggaa tatacctttt
     1441 cggggggata tgcaaaaaca cccttgttgg gaaataccca aagatcgaac aaaaactgat
     1501 aaaacgatct tccaataatt ctttgatgcc agatgaacct gcagtctgaa tcaccgagta
     1561 ggcactttat cgtgaattaa agaacacatg accctttttt ttgaaatttt acgctcttat
     1621 gtatgaattg gagtttcgct ctttttataa tttaagtt
//
LOCUS       DAOQDO010001669         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80477_length_1656_cov_2.260462, whole genome shotgun sequence.
ACCESSION   DAOQDO010001669 DAOQDO010000000
VERSION     DAOQDO010001669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80477_length_1656_cov_2.260462"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1656
                     /locus_tag="PKZ53_21645"
     CDS             <1..>1656
                     /locus_tag="PKZ53_21645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ43102.1"
                     /translation="PQNPTGAHLIGLRDELVEGLATKLWILQAATVFVLLICCANVAN
                     LYFAQVFQQQGELAIRTALGASRWQLLRQLMVQSLLLSGLGAIAGIAVAYWSQKPLLA
                     LSPLAPGPAASAITQIHTQVNLDLTSLGFALAVTLLVAILFGLIPALKASNTNPHLIL
                     RETTRTGGMTARRQHWLNGLVVIEFALAIVLLAGAGLMIRSFLALQNVQWGFSLKQTL
                     TFDLNFSESRYPNQAQRLAFIQTAMTNLRNIQGVQSVGATTVQPLYRGTFLSSYSIEG
                     RQVPDPPGYYICHHRRVLPGYFQTIGIPILSGSDFSGLERIDTEPVILISKMMADRFW
                     PGENPIGRRIKSGRLDSNGPWFTVVGVVGDVKESVDPNDGEVVGTWYLPYTQQRSTAF
                     SSISFVVKTDGAPENVTAAIREAIWAIDRDQAIYNVATFEHLAKESFRPERFSTTLIL
                     LFGVVGLILAVVGIYGVVSYSLVSRIPEIGIRLAFGASPLDIFKLILQHGASLIAAGL
                     TLGLIAAGLLSQYLKSQLFGVSELDPASYALASFLLAMVALVA"
BASE COUNT          346 a          421 c          462 g          427 t
ORIGIN      
        1 acccgcaaaa tccaaccggc gcgcacctga tcggtctgcg cgacgaactg gttgaagggc
       61 tggccaccaa attgtggatc ctgcaggcgg ccacggtgtt tgtgcttttg atttgttgtg
      121 ccaatgtggc caatctctat tttgcccagg tatttcaaca acaaggggag cttgccatcc
      181 ggacggcgct tggtgcgtcg aggtggcaat tattgcggca gttgatggtt caaagcctgc
      241 tcctgtcagg gttgggtgcc attgcaggga ttgccgtggc gtactggagc caaaaaccat
      301 tgctggcctt gagcccgttg gcaccaggac cggcagcctc ggccattacc caaattcaca
      361 ctcaggtcaa tttggatcta acctcgcttg ggtttgccct cgctgtcacc ctgctggttg
      421 caattctgtt tggcctgatc ccggcgctca aagcctcaaa taccaaccca cacctcatat
      481 tgcgcgagac aacccgaaca ggcgggatga cggcccggcg tcagcactgg ttaaatgggc
      541 tggtggtgat tgaatttgcg ctggcaattg tcctgctggc tggggccggg ctgatgattc
      601 gcagttttct ggcgttacaa aatgttcagt ggggcttttc actcaaacaa acgctgacat
      661 ttgacctgaa tttttcagag agtcgctacc caaatcaggc gcaacgtctg gcatttattc
      721 aaacagccat gaccaacctt cggaatatac agggggttca aagcgtcggc gccaccacgg
      781 tccagccgtt gtatcgaggt actttcctta gcagctacag catcgaaggc cgtcaggttc
      841 ccgaccctcc gggctattat atttgccatc accgccgggt cctgccaggg tatttccaaa
      901 cgattgggat tccaatttta agtggaagcg acttctctgg cctggaacgc attgatacag
      961 agccagttat cctgattagc aaaatgatgg ctgaccggtt ttggcccggg gaaaatccaa
     1021 ttggaagacg gattaagagt ggacgactgg attcgaatgg cccctggttt accgttgtgg
     1081 gggtggtggg tgacgtcaag gaatcagttg acccaaatga tggtgaagtg gtcggcacct
     1141 ggtatctgcc ctacacccag cagcgatcaa cggccttttc aagcatcagt tttgtcgtca
     1201 aaaccgatgg tgcgccagaa aatgtaacgg ctgccattcg tgaagcaatc tgggcgattg
     1261 accgcgatca ggccatttac aatgtagcta catttgaaca tctggccaaa gagtcgttcc
     1321 gacccgaacg ctttagtaca actttgattt tactgtttgg cgtcgtaggt ctgattctgg
     1381 cggtggtggg gatttatggc gtggtgtcgt actcgctggt ttcaagaatt ccggaaattg
     1441 ggatccgtct cgcgtttggg gcgtcgccac ttgatatctt caaattgatt ttacagcacg
     1501 gggccagcct gattgccgct ggcctgacgc ttggtctgat tgcagcggga ttgctttcgc
     1561 agtatctgaa aagccagctt ttcggcgttt ccgagctgga tccagcgtcg tacgccctgg
     1621 ccagtttctt acttgcgatg gtagcgttgg tcgcct
//
LOCUS       DAOQDO010001670         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80716_length_1653_cov_3.449312, whole genome shotgun sequence.
ACCESSION   DAOQDO010001670 DAOQDO010000000
VERSION     DAOQDO010001670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80716_length_1653_cov_3.449312"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1550
                     /locus_tag="PKZ53_21650"
     CDS             72..1550
                     /locus_tag="PKZ53_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43103.1"
                     /translation="MLDQSRTVCDQGITVSPGKADLYLVSANISILQAQTKTEKGEDP
                     VLELEGCIEKARAALAVDPHNAFGFRMLGEAFRQRGIYVMDKGQNPDRDFQSAIDFNR
                     QAVAARPNHGVNHFYLGWAYWSLGEYQLVNGESPIQAYDLAAGCYTKVLELSQPTARI
                     YNSLANVFLSKGEYLAIQGQNPIPVLNQAIETYQRALELNPNLYGALINTATAFTDII
                     AYKIDHGQDPSAEFEASIRFYERGIAANPKNPIAYNNFAQVYMQKAQYLADQKQDPLP
                     LLNQALETLAKGLKIRDNDAYTYLYIGRAHLIEASYQTSIKHDPMAALTPAIQAFQHA
                     QELNPTFFEAWSHEGQAQAIALEWLLDTGQPFSEHIFAKAQTVFRKTIELNKEYSAAH
                     NQLAKLQWLRARWLIAQKKEALATLNSGLEEIKQAIDLKGDNPEFHITQGKLFALKAQ
                     IQKDPVSANQLREQARQSFTKAVELNPEVKADVDEILKDLNR"
BASE COUNT          453 a          435 c          387 g          378 t
ORIGIN      
        1 cacctgattc aaccgttttc tttcttgaaa aaacagcttg acatagaatg tccgggcagc
       61 ccttccagga gatgcttgac caatcgcgga ctgtctgtga ccaggggatt accgtatcgc
      121 ctgggaaagc tgatttgtat ctggtcagtg ccaatatttc gatccttcag gctcagacca
      181 aaacggaaaa aggggaagac ccggtccttg agttggaagg ctgtatcgaa aaagccaggg
      241 ctgccctggc cgttgatccc cacaatgcct ttggatttcg gatgctgggt gaagcctttc
      301 gccagcgcgg aatctatgtc atggacaaag gccaaaatcc agatcgtgat tttcaatcgg
      361 ccattgactt taaccggcag gcggtcgcgg ccaggcccaa tcacggcgtc aatcatttct
      421 atttggggtg ggcctactgg tcacttggag aataccagct cgtcaatgga gagagtccga
      481 ttcaagccta tgatttagcc gccggatgct atacaaaagt tcttgagtta tcacagccaa
      541 cagccagaat ttacaatagt ctggccaatg tatttctctc aaagggggaa tacctggcga
      601 ttcaggggca aaacccaatt cccgtactca atcaggcgat tgaaacatat cagcgcgcgc
      661 tggaactaaa cccgaacctg tatggtgcgc tcatcaatac cgcaactgca tttaccgaca
      721 ttattgccta caaaatagac cacggtcagg atccatcggc tgaatttgaa gcaagcatcc
      781 ggttttatga acggggaatt gctgccaacc caaaaaaccc gattgcctac aataattttg
      841 cccaggtgta tatgcagaag gcccagtatc tggctgatca gaagcaagat ccgcttccgc
      901 tgctcaatca ggcgcttgaa accctggcca aggggctcaa gattcgggat aacgacgcct
      961 atacctatct ctatatcggg cgggcccatt tgatcgaggc cagttaccaa accagtatca
     1021 aacacgatcc aatggctgct ttgacccctg ccattcaggc atttcaacat gcacaggaac
     1081 tcaacccgac attctttgaa gcctggtcgc acgaaggtca ggcccaggcc attgctctgg
     1141 agtggctgct cgacaccggt cagccgtttt cagagcacat ttttgccaaa gcccaaactg
     1201 tttttcgaaa aacaatcgaa ttaaacaaag aatattcagc cgctcataac cagcttgcca
     1261 aattacagtg gctccgggct cgctggctca ttgcccagaa gaaagaggct ctggcaacac
     1321 tgaattcagg tctggaggaa atcaaacaag cgattgatct caaaggggat aacccggaat
     1381 ttcacattac ccagggaaaa ctgtttgcat tgaaagctca aatccaaaaa gatccagttt
     1441 cggcaaacca gttgcgcgag caggcccggc agtcatttac caaggccgtt gaactcaatc
     1501 ccgaagtcaa agccgatgtt gacgagatac tcaaagatct gaaccgataa ccgggttccg
     1561 ggttccgggt tccgggtttt cgaaaagcag gccgtaaacg acctgtctca attcaaccac
     1621 cacttccacc gcgaaaacct ttgtcacctt gtt
//
LOCUS       DAOQDO010001671         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80739_length_1653_cov_2.819149, whole genome shotgun sequence.
ACCESSION   DAOQDO010001671 DAOQDO010000000
VERSION     DAOQDO010001671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80739_length_1653_cov_2.819149"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..>1653)
                     /locus_tag="PKZ53_21655"
     CDS             complement(155..>1653)
                     /locus_tag="PKZ53_21655"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNJ43104.1"
                     /translation="IITILLFALYKFTAHARSDLPLPTDRAFALICVTLVAQTMLIWL
                     GINISRKFGGTAGMSTDLSLYEFAIPYAAATLIVALLLDENLALLCTLVVGLLAGVMT
                     GGSMELCVFAILSGSAAAYSVGQYRQRNTIIRAGVFVGGINLFAVGAVICIAGSSAML
                     NNTLLTMLLCGIASGLITAAFASFAVPVCESMFGILTDVKLLELSNADLPLLRELAMR
                     APGTQQHSVMVSSLAQEAAEAIGANALLVRIGCYYHDIGKLLAPEMFIENQGGGPNPH
                     DKMEPRRSASVITGHVRKGIIMAREANLPKEIIDLIPQHHGTRKLHYFFNKASERAAT
                     RGESVDETDYRYPGPKPQTKEAAIVMMADSAEAAARSLDEPTPENIGQIVKRILDDII
                     ADGQLDECLMTMREITLVREQLVRTLCNIYHHRVVYPGFNSSCADSEEIGLCLMTSDE
                     TERIEPVEVEFNPEPELTPEPVNISTAARIPSRLKTGEIPPIQGITRA"
BASE COUNT          414 a          434 c          420 g          385 t
ORIGIN      
        1 gatggagcgg cgatggatcc caaatttgat cgttcccggc ctcaagcacc agcctcactt
       61 cagccaccgc tacgcagcga agaaaggtca accgaaccta atactttcgg agaaccagga
      121 aacagacatc aaccactctc tcaattcttc attctcaggc gcgggtaatg ccttgaattg
      181 ggggaatttc acctgttttt aaccggctgg ggatccgcgc ggcggtggat atattgactg
      241 gttcaggggt taattccggc tctggattga attcaacttc aacgggttca attcgctcgg
      301 tttcatcaga agtcatcaga cacaggccga tttcttcaga gtcagcacag ctactattaa
      361 agcctgggta aacaacccga tggtgataga tattgcacag tgttctgacc agttgctcgc
      421 gaaccagggt tatttcccgc atggtcatta aacattcgtc caactggcca tcagcaatga
      481 tgtcatccag aatccgcttt acaatttgcc caatgttttc gggtgtgggt tcatcaagtg
      541 atcgggcggc ggcttcggca ctgtcagcca tcatgacaat ggccgcttct ttggtttggg
      601 gtttgggacc tggataccgg taatcggttt catcgactga ttcgccgcgc gtcgctgccc
      661 gttctgatgc tttattaaaa aagtaatgca gtttgcgggt gccgtggtgc tggggaatta
      721 aatcaataat ctcttttggc aggttggcct cacgggccat aataattcct ttgcggacat
      781 ggccggtgat cacactggca cttcgacggg gctccatttt gtcgtgggga ttgggacctc
      841 cgccctggtt ttcaataaac atttctgggg ccaataattt cccgatatcg tggtaatagc
      901 acccgatgcg aaccagcaag gcattggccc caatggcctc ggcagcttcc tgggccaggc
      961 tgctgaccat aaccgaatgt tgctgagttc ctggcgcccg catggccagt tcacgcaaga
     1021 gcggcaaatc agcgtttgag agttcaagca atttgacatc agtcagaatg ccaaacattg
     1081 actcgcagac cggcacggca aaactggcaa aagcggcggt gatcaatccg ctggcaatcc
     1141 cgcacaacag cattgtcagg agtgtgttgt tcagcatggc gctgctcccg gcaatacaga
     1201 tgacagcccc gaccgcaaat aaattgatcc ctccaacaaa aacacctgcc cggataatgg
     1261 tatttcgctg gcgatactgg ccaacgctat atgcagcggc acttccgctt aaaatggcaa
     1321 aaacgcataa ctccatgctc ccccctgtca tcaccccggc cagaaggccc accaccaggg
     1381 tacagagcaa ggccaggttt tcatcgagga gcaaggcgac aatcaaggtt gcagcggcat
     1441 acggaattgc aaattcatag agcgacaggt cagttgacat ccccgctgta ccgccaaatt
     1501 tccgactgat gttgattccc agccagataa gcatggtttg ggcgaccagg gtcacacaaa
     1561 tcaatgcaaa agcacgatct gtcggcaacg ggaggtcaga gcgggcatgc gccgtaaatt
     1621 tatagagcgc aaataataaa atggtgataa tcc
//
LOCUS       DAOQDO010001672         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80762_length_1653_cov_2.204631, whole genome shotgun sequence.
ACCESSION   DAOQDO010001672 DAOQDO010000000
VERSION     DAOQDO010001672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80762_length_1653_cov_2.204631"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..904
                     /locus_tag="PKZ53_21660"
     CDS             29..904
                     /locus_tag="PKZ53_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43105.1"
                     /translation="MKKPLLAAFLVVASWTASASAYTVILKDGRRLEVQENYRIVNDI
                     AVFVLADGKRFSVSVNNVDIAKTEMANHQGIGEFVQRAGAPLPILDSDVPAVKPTPAM
                     KDKAAVARAKTPITRTLVNADFETYKQRRGDSKKDKDTTPETSPTSEVAAATPAFQPL
                     PIDPVLDRKSEAYWRERARPLLTEMAVQEELIGILQNQLSQLEANRGRGQTSYSTIQT
                     PGGVVINGNGGYYNPGSTVLIPQTNTSGQDFETKVRERLMEARLNLTAVQIRYNALTE
                     EARRAGVPPGWTRWQ"
     gene            1015..>1653
                     /locus_tag="PKZ53_21665"
     CDS             1015..>1653
                     /locus_tag="PKZ53_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43106.1"
                     /translation="MKQFFRVQRSIVTTRLIKGKSKWAIVGVLLFLACSWGQVAADSD
                     DVFEQSFEVGSNANIKVDNPAGTIAVEVWGEDLVHVVAARKGGGSQRVLQSEVSFWKG
                     ANQITIVCPAKLDAGPINLKIYIPRRTHLRLTSNQGDVEVLGPVASASIETQTGNVLI
                     ESPSSQDAEVIMRSTAGSVTCGVHMTVCDSSTPTLVEGKLGKGGSAIVIRSTS"
BASE COUNT          413 a          400 c          453 g          387 t
ORIGIN      
        1 aaatcccaca atttttcgtg gagggcctat gaagaaacca cttctggctg cgtttcttgt
       61 ggtcgcttcc tggactgcca gcgcgtcggc atacacggtt attctcaagg atggccgacg
      121 gcttgaagtt caagagaatt accgcatcgt taatgatatt gctgttttcg tgctggccga
      181 cgggaaacgg ttctcagtgt cagtcaacaa tgttgatatt gccaagacag aaatggccaa
      241 ccatcaaggg attggtgaat ttgtccagcg agccggagca ccgctcccga ttcttgattc
      301 agatgtgcct gcggtaaaac caactccagc aatgaaagat aaagccgctg ttgcccgggc
      361 aaagacaccg atcacgcgta cgctggtcaa tgctgacttt gagacctaca agcaacgtcg
      421 tggcgattca aagaaggaca aggatacgac acctgaaacg tctccaactt ctgaagttgc
      481 tgctgccaca cctgccttcc agccacttcc gattgatcca gtcctggatc gaaagtccga
      541 agcctactgg cgtgagcgag cccgaccact cctgaccgaa atggcagtgc aagaagagtt
      601 gatcggtatt ttgcaaaatc agctctcaca actggaagcc aatcggggac gtgggcaaac
      661 atcatacagc accatccaaa cccctggtgg tgtggtgatc aatggcaatg gtgggtatta
      721 caatcctggg agcactgttt tgatcccaca aaccaatacc agtggccagg attttgaaac
      781 caaagttcga gagcgtttga tggaagcacg gttgaatctg acggctgtcc aaattcgcta
      841 caatgccttg accgaagagg cccgccgggc cggagttcca ccaggatgga ctcgctggca
      901 gtaggcaggt gactggtgct cagtgcttgg tacctggtgc ccggtgcttg gagtattcaa
      961 agaacccgga acccggaacc ccaatttctg ccccgtcttg aggaggacta cattgtgaaa
     1021 cagttctttc gtgttcaaag aagtatcgtg accacacgat tgatcaaggg gaagagcaag
     1081 tgggcaatag tcggtgtact tttattttta gcttgttcct gggggcaggt agcggctgat
     1141 tcagatgatg tcttcgaaca aagctttgag gttggctcga atgccaatat caaagttgat
     1201 aatccggctg gcacgattgc ggtggaggtc tggggagaag acctggttca tgttgtggcg
     1261 gcccgaaaag gtggcgggag ccagagagtt ctgcaatctg aggtctcgtt ctggaaaggg
     1321 gccaatcaga ttaccatcgt ttgcccagcc aaactggatg ctggtccaat caacttaaaa
     1381 atctatattc cacgccggac ccacctccga ttgacctcca atcaagggga tgttgaggtt
     1441 ctggggccag tcgcgagtgc cagtattgaa acgcagactg gaaacgtgct gattgaatcc
     1501 ccaagttcac aggatgccga agttatcatg cgctcgactg ctggttcggt gacctgtggt
     1561 gttcacatga cagtgtgtga ttcatcaact cctaccctcg tagaaggaaa actggggaaa
     1621 ggtggttcgg cgattgtcat tcgcagcacg agt
//
LOCUS       DAOQDO010001673         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80777_length_1653_cov_1.974343, whole genome shotgun sequence.
ACCESSION   DAOQDO010001673 DAOQDO010000000
VERSION     DAOQDO010001673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80777_length_1653_cov_1.974343"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKZ53_21670"
     CDS             <1..325
                     /locus_tag="PKZ53_21670"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNJ43107.1"
                     /translation="MRLQDAPFPVDPFFRSLALAFGRCGLAVIMNSLEADHSLGLTDV
                     ATAGGQILKQAVESPPMLGGVVSRAPLTTEVKPRQKIVLPENLDSTITYTVTQLMPLG
                     ASRRA"
     gene            494..883
                     /locus_tag="PKZ53_21675"
     CDS             494..883
                     /locus_tag="PKZ53_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ43108.1"
                     /translation="MRALVIDDSRAIRSVLTKMLKELGFEVFEAGQGLDGLDQLRKVG
                     KIDLALVDWNMPEMNGYQFVCAVRSDPAYNNLRLMMVTTESELSQMVSVLNAGADEYV
                     MKPFTREIIFEKLCLLNLSDSGKNPTN"
     gene            900..>1653
                     /locus_tag="PKZ53_21680"
     CDS             900..>1653
                     /locus_tag="PKZ53_21680"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNJ43109.1"
                     /translation="MVSFSSSEFSYIQKLIYEQSAIVIEPGKEYLVEARLSPLAHQTG
                     CASISDLITRLRLEPNTGLHRVAVEAMTTNETSFFRDVTPFEALKKEVIPQLIARRAA
                     EKTLSIWCAASSTGQEPYSLAMLLREHFPVLNSWKLYLMASDISTEVLARARQGKFSQ
                     LEVNRGLPASMLVKYFHREGLEWQISDQLRRQIEFREINLAKEWPYIPMLDLVLMRNV
                     LIYFDIKTKKEILEKTRRRLKPDGVLFLGTAET"
BASE COUNT          390 a          423 c          430 g          410 t
ORIGIN      
        1 gatgcgctta caggatgcgc cgttcccggt ggatcccttc tttcgcagcc tggcactggc
       61 ctttggtcgg tgcggtctgg ccgtcattat gaatagcctg gaggctgacc attcgcttgg
      121 cttaaccgac gttgccaccg ccgggggaca aatcctgaag caagcggtcg aatcaccccc
      181 gatgttgggc ggtgttgtca gtcgggcacc cctcacaaca gaggtcaaac cccgtcagaa
      241 aatcgtgttg cctgaaaatc tggattcaac catcacctac actgtgaccc agttgatgcc
      301 actgggcgcc agccgcaggg cctgagggta agtgggtgga gtgagagccc ctgtccgctt
      361 cacgtttcat ctgatgtggc ctccgtacac tcagttcagc aacctggcat ctcaccagac
      421 ggttatttcc cggctcagac ctgtctgccg ctggttctgg tgaatgcact ctctgtgagc
      481 aaaacgagga tctatgcgcg cattagtcat tgatgattca cgagcaattc gttccgtttt
      541 aaccaaaatg ttgaaggaac tcgggtttga agtttttgaa gctggtcagg ggttggatgg
      601 attggatcag cttcgcaaag tcggaaaaat tgatctggcc ctggttgact ggaatatgcc
      661 ggagatgaat ggatatcagt ttgtttgtgc ggtgagatcc gacccggctt acaacaacct
      721 ccgcttgatg atggtcacca ccgaatcaga gttgagccag atggtcagtg tgctcaatgc
      781 cggcgccgac gaatatgtga tgaaaccctt cacccgagaa ataatttttg aaaagctgtg
      841 cctgctcaac ctttccgatt caggaaagaa cccaacaaac tgaaggcgat gtgtttgcta
      901 tggtatcttt ttcaagttct gaattttctt acattcaaaa gctgatttat gagcaatcag
      961 ctatcgtcat cgagcccggc aaagaatatc tggtcgaagc ccgattgtcg ccactggcac
     1021 atcagaccgg ctgcgcctcg atttcagact tgatcacccg gttgcggctg gagccaaata
     1081 ccgggttgca tcgggttgcc gttgaagcca tgacaaccaa cgagacaagc tttttccggg
     1141 atgtgacacc gtttgaagct ctcaaaaaag aagtgattcc gcagcttatt gcccggcggg
     1201 cggctgagaa aacgctgtct atctggtgtg ctgccagctc aaccggtcag gaaccatatt
     1261 cgctggccat gctcttgcgc gagcattttc cggtgctcaa ctcctggaag ctgtacttga
     1321 tggccagcga tatttcaacc gaagttctgg cccgcgcccg acagggaaaa tttagccagc
     1381 tcgaagtcaa tcgggggctt cctgcctcga tgctggtcaa atatttccac cgcgagggcc
     1441 tggagtggca aatctcagat cagctccgcc gccaaattga attccgcgaa atcaatctcg
     1501 ccaaagagtg gccctatatt ccaatgcttg atctggtcct gatgcgcaat gttctgattt
     1561 attttgatat taaaaccaaa aaagaaattc ttgaaaaaac ccgaaggcgg ttaaagccgg
     1621 acggcgtttt gtttttggga acagccgaaa cca
//
LOCUS       DAOQDO010001674         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80901_length_1651_cov_3.320175, whole genome shotgun sequence.
ACCESSION   DAOQDO010001674 DAOQDO010000000
VERSION     DAOQDO010001674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80901_length_1651_cov_3.320175"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1414)
                     /locus_tag="PKZ53_21685"
     CDS             complement(152..1414)
                     /locus_tag="PKZ53_21685"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNJ43110.1"
                     /translation="MRRRNFIALFLMIFAIDSATMLSWNSVEAATVQQDQPTTHKLPA
                     GGKVTIRQVFGELSVIGVSGDTVTIEAKNDDGEPAEVKVMQEAQDLLISTEVDGPRRK
                     KRAISLEVKIPSSANLTIESQAGEISVLDITGQTAIRAGGGEIKLVNVGSLVAATGGG
                     EVTVQKVTGTVEIRTGGGELQVSDVSGAVSLITGGGEVTTSNTGDLEVRTGGGDVVVQ
                     DVHGSASIATGSGDLVVSKVEGNCELKSGHGDASLRDISGNVALSNVSGDVVAQNIGG
                     NMAVASISADMDLKCVKGRVEVRNASGSVQLAHIGGDIEVTSTSGDVTLSTEIAQGNR
                     YRIKTTSGNLLMMVPESASGFSVNLSSYTGTVESNLKLEGSTSTEKKLTGQYGEGRGE
                     IVLDTFSGSVSLKKGAVKNQSDCGKKSE"
BASE COUNT          381 a          443 c          390 g          437 t
ORIGIN      
        1 ctgccaaaac tatcgcagtt gcccggcaaa gagcaaccct tttgaaaagt gaggtgttca
       61 acacacgctg gtcaataaaa aaagaaggtg gccaattttg accacctttc atcatcaaca
      121 tgatccactt accacttgct cttctgattt gctattcgct cttttttcca caatcggact
      181 ggttcttgac ggcgcctttc ttgagtgaga cagatccgct aaaggtatca agcacgattt
      241 caccgcgacc ttcgccatat tggccggtca atttcttttc ggtcgaagtt gagccttcca
      301 atttcaagtt ggattcaacc gtgcccgtgt atgaagaaag gttgacggaa aaaccagagg
      361 ctgactcggg caccatcatc aacagatttc cagaagtggt tttgatgcga taccggtttc
      421 cctgggcaat ttcagttgaa agcgtcacat cgccactggt tgaagtgact tcaatatctc
      481 caccaatatg cgccagttgc accgacccgc tggcattgcg aacttcaacc cggcctttga
      541 cacatttcag gtccatatcg gcactgatgg aggcgaccgc catattgccg ccgatgtttt
      601 gggcaaccac atcgccgctc acattcgaga gcgccacgtt gccggaaata tccctgagac
      661 tggcgtcacc gtgaccgctt ttgagttcgc aattgccttc aactttggaa acaaccagat
      721 cacctgaacc cgtggcaatg gaagccgagc cgtgaacatc ctgcaccacg acatctccac
      781 cgccagttcg gacttcgagg tctccggtat tggatgtggt gacttcgccg ccgccagtga
      841 ttaaagaaac ggcgcctgaa acatccgaga cttgcagttc accgccgccc gttctgattt
      901 caaccgtgcc ggttactttt tgaaccgtga cttcgccacc accagtggct gccaccagcg
      961 agccaacatt gaccagttta atttcaccgc caccagctct gatggctgtc tggccagtga
     1021 tatccaaaac cgaaatttca cctgcctggg attcaatcgt gagattggcc gacgaaggga
     1081 ttttgacttc gagtgaaatt gcccgttttt tcctgcgggg gccatcaacc tcagttgaaa
     1141 tcaacaggtc ctgtgcctct tgcatcactt tgacttcggc tggctcgcca tcatcatttt
     1201 tggcttcgat ggtcaccgta tcaccagaaa ccccaataac cgagagttca ccaaaaacct
     1261 ggcggatcgt gacttttccc ccagccggta gtttgtgtgt cgtcggttga tcttgctgga
     1321 cggttgcggc ttcaaccgaa ttccaggaca gcatggtggc gctgtcaatg gcgaagatca
     1381 tcagaaacag tgcgataaag ttgcgtctcc tcataatatt tcacagtcct tttttgttgt
     1441 gtcgcttcag attcagtcag gttcacggtc ttttttaggg ttcagggttc aggggggtca
     1501 ggagggatga gggatgaggg atgagggatg aaaaaatccc ctgttacctt gtcatcttgt
     1561 cagaatcttg ggcgggatca gttgtttcat cgcctaaaac cctgaaccct gaaccctgtt
     1621 tcattttagg gtctcaattc aaccggtttt t
//
LOCUS       DAOQDO010001675         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80944_length_1651_cov_2.242481, whole genome shotgun sequence.
ACCESSION   DAOQDO010001675 DAOQDO010000000
VERSION     DAOQDO010001675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80944_length_1651_cov_2.242481"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKZ53_21690"
     CDS             <1..573
                     /locus_tag="PKZ53_21690"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HNJ43111.1"
                     /translation="EIELLASWLHGDYNSHAQADRDAAGKDPLVFKHRKITMHLRPCP
                     IEGLPGPTFYIEQHASEAPDQPYRQRIYQIFRKRGELTVRTYELIGGREAAKPYIMGY
                     KNPEVLKSITAAKLTPVCDLVVHRKGKEFVGKTPMPCPNMFNGAVEVTSDVTIGPDYL
                     FSLDRGWAADGQQAWGPNDGVAFEFRRVAE"
     gene            complement(704..>1651)
                     /locus_tag="PKZ53_21695"
     CDS             complement(704..>1651)
                     /locus_tag="PKZ53_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43112.1"
                     /translation="AAILAYEDSDADGKIDGTVTRQLITTDDLALRDVTEVSGGMAID
                     QRDRLFFTARNTLKNITSIQLATDTDADGYPDKLQLLPFVVTPTTTGLSGLARLRIRE
                     SDDRLFFSSVTVQDTRFIPLAGALFTIQDLDGNNQGDGLIRVAQDLSLNSVSPNITNS
                     GLEFGPDGEIYVIGAFPGFQAGPLVLPQYTLYQLQPASDGLAMPQVFDLGVPVDLTNK
                     RVGFLTGLARGTSPTRFQPRLSSFSLSKKVLSLNGFRFRKGLTLTVDGAPVAPSAYTV
                     TPTQIVLAKGKKLLPRRKSAVIRLVNPDGQSAEITVSRL"
BASE COUNT          408 a          434 c          418 g          391 t
ORIGIN      
        1 gaaatcgaac tcttagcctc gtggctgcac ggtgattaca actcgcatgc tcaggcggat
       61 cgcgatgccg cagggaaaga cccactggtt tttaagcatc gaaaaatcac aatgcacctc
      121 aggccctgtc cgattgaagg actgcctggc ccaacgtttt atatcgaaca acacgcttct
      181 gaggctccgg atcaacccta tcgccagcgg atttatcaaa tctttcggaa acgcggggaa
      241 ctgaccgttc gaacctatga actcatcgga gggcgtgaag cggccaaacc ttacattatg
      301 gggtataaga atcctgaggt tttaaagtca attaccgccg cgaaactcac gccggtatgc
      361 gatttagtgg ttcatcgcaa aggcaaggaa tttgtcggaa aaacaccgat gccctgcccg
      421 aacatgttta atggtgcggt tgaagtcact tcggatgtca cgattggtcc agattacctc
      481 tttagccttg atcgtggttg ggcagccgac ggccagcaag cctggggtcc aaatgatggt
      541 gtggcttttg aatttcggcg ggttgctgaa tagatccgcc ggcaaacctg ttttgatact
      601 ctcccagact ttttcttgat tcatcggggt gctggccagt ttcagggtat caccccgata
      661 tttttttcag acacctggtc ttccagttcc gccagcgttc ccgtcacaaa cgactcactg
      721 taatttccgc actttgtcca tcagggttta ccagccgaat cactgctgat ttgcgcctgg
      781 gaaggagctt tttccctttg gcaaggacaa tctgggtggg agtcaccgta tacgccgaag
      841 gggcaaccgg tgcgccatct accgtcaggg ttaagccttt tctaaatcgg aaaccattga
      901 gggaaaggac tttcttactg agtgagaacg aactgaggcg aggttgaaac cgggtgggtg
      961 acgtcccccg agccaaaccg gtcagaaacc caacccgttt gtttgtcagg tcaacgggta
     1021 cgcctaaatc aaagacctgg ggcatggcca gcccatctga cgctggctga agctgataca
     1081 gggtatattg aggcaagacc agcggacccg cctgaaatcc aggaaaagcc ccaatcacat
     1141 aaatttcccc gtcaggtcca aactcaagcc ctgaattggt aatatttgga ctcaccgaat
     1201 tgagactgag gtcttgcgcg acccggatca acccatctcc ctgattgtta ccatccaggt
     1261 cctggatggt aaacagagct ccggccagcg ggataaaccg cgtgtcctga acggtcacac
     1321 tcgaaaaaaa cagacggtca tctgattcac ggattcggag cctggccaaa ccactgagcc
     1381 cagtggtggt cggtgtaaca acaaagggca acagttgcag tttatccgga tatccatcag
     1441 catcagtatc ggttgccagt tggattgaag taatgttttt cagagtattt cgggccgtaa
     1501 aaaacaatcg atcccgctga tcaatggcca ttccaccact gacttcggtc acatcacgca
     1561 gtgccagatc gtcagtcgtg atcaattgcc gggtcacagt cccgtcaatt tttccatctg
     1621 catccgaatc ctcataggcg aggatggctg c
//
LOCUS       DAOQDO010001676         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_80981_length_1650_cov_3.780564, whole genome shotgun sequence.
ACCESSION   DAOQDO010001676 DAOQDO010000000
VERSION     DAOQDO010001676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80981_length_1650_cov_3.780564"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PKZ53_21700"
     CDS             complement(<1..1192)
                     /locus_tag="PKZ53_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43113.1"
                     /translation="MPGIFRRQVFKAWMGLLVMLMQGVVPVLAQTPQPASQSTPSTES
                     LARYLPDNQLLFLEIENIPGMVDELFKSEGIRKAVTKLSEGKITSPAQIEAKLQELGF
                     PDKATLSQIQIGFGLRIPATDVSGLARRQANTASAVQVLRNLHSAQVVYQTSLGKGNF
                     ASSLKLLGPGGEEAVDFLDESIVNLQTTPRNGYLLGPLKVTPPGENTAPTYSISAFPA
                     VKEGNAQTGDDCFYVDETGVIRHSGSATKPAGPESEPVESIPSVPSPSPEMVFLIKAP
                     TVQAATQCITALIKLENYGRAGESKTEEVGKNLVTLFPNTSLKPEDAFAYTVQPDGLV
                     MVSAFGQLKRLLTTGSEAPKLFNLKDFQVSRQDIGPGWPARFYLNTREVGGMFEYLIQ
                     AMNKQ"
     gene            complement(1427..>1650)
                     /locus_tag="PKZ53_21705"
     CDS             complement(1427..>1650)
                     /locus_tag="PKZ53_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43114.1"
                     /translation="DYQRKQARSIEEYETEIKEGENTGTSLMRAGLLELDKLTKPNLQ
                     AAIEFAKDAEEGQTDNQKESGEGAEKNSK"
BASE COUNT          375 a          403 c          409 g          463 t
ORIGIN      
        1 attgcttgtt catcgcttga atgaggtatt caaacattcc gccaacttct cgggtattga
       61 ggtaaaaccg ggctggccac ccgggaccaa tatcctgacg agatacctgg aaatccttga
      121 gattaaataa tttcggagct tcgcttccgg ttgtaagcaa gcgttttaac tgtccaaatg
      181 cactgaccat caccagtcca tcgggttgaa ccgtgtaggc aaacgcgtct tccggcttga
      241 ggctggtatt gggaaaaagg gtcaccagat tttttccgac ttcttcggtt tttgattctc
      301 cggcacggcc ataattttcc agcttgatca aggcagtgat gcactgagtt gcggcttgaa
      361 cggttggagc tttgatcaaa aaaaccattt ccggtgatgg cgagggcacg cttggaattg
      421 attcaacggg ttcactttct ggtcctgccg gtttggtggc tgatcccgaa tgccgaatca
      481 ccccagtctc atccacataa aaacaatcat ctccggtttg agcatttccc tctttaacgg
      541 ctggaaatgc gctgattgaa taggtcggcg cggtgttctc gccgggtgga gtgactttga
      601 gtggtccaag caaataccca ttcctgggtg tcgtttgcag gttcacaatt gactcatcca
      661 aaaaatctac cgcttcttcc ccgccaggac ctaaaagttt caatgaactg gcaaaattgc
      721 ctttgccgag cgaggtttga tacacaacct gggctgaatg gaggtttcgc aaaacctgta
      781 cggctgatgc ggtattggcc tgccgacgag ccaaacctga cacatcggtt gctggtattc
      841 gcaacccaaa tccaatttga atctggctca aagttgcctt gtcgggaaat cccagttcct
      901 ggagtttagc ctcaatttgg gcggggctgg tgattttgcc ttccgaaagt ttggtcacgg
      961 ctttgcggat gccctcagac ttaaataatt catccaccat ccctgggatg ttttcaatct
     1021 caagaaacaa aagctgattg tccggcagat accgggccag tgattcggtg ctcggggtag
     1081 attggctggc tggttggggt gtttgggcca ggaccggcac cacaccttgc atcaacatca
     1141 ccagcagtcc catccacgct ttgaaaactt gacgacgaaa tatcccaggc atagaaaaat
     1201 cctttcggtt gagtgaaaag agttcggtac tttttaggga cccggaagaa aataagatcc
     1261 caatgaaatc gagttcaggg tttgaggcga tgaagcgact gatctcctca cgatgagaac
     1321 tggggaacca gcagctacgg ctcatgctgg attatcttgc tctgaaccct aaaccctgaa
     1381 ccctgaaccc tggtggtatg atatttccat ccgactccct taaaagctat ttgctgtttt
     1441 tctcagcgcc ttcgccgctt tctttctggt tgtcggtttg gccttcttcg gcatctttgg
     1501 caaattcaat tgctgcctga aggttgggtt tggtgagttt gtcgagttcg agtaacccgg
     1561 cacgcatcag tgacgttccg gtgttttcac cttctttgat ttcggtttca tattcttcga
     1621 ttgaacgcgc ctgtttccgc tgatagtcat
//
LOCUS       DAOQDO010001677         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81014_length_1650_cov_2.550470, whole genome shotgun sequence.
ACCESSION   DAOQDO010001677 DAOQDO010000000
VERSION     DAOQDO010001677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81014_length_1650_cov_2.550470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKZ53_21710"
     CDS             complement(<1..452)
                     /locus_tag="PKZ53_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43115.1"
                     /translation="MASDTRQMLINLEQFLTESVKANYWLTPNDIAPNSIARHLLDEL
                     ARKRFIWVGNETYVPNKIVVNAPNPTPEKLEEYEVLFNSVVFMKYLYDYITGSGFKLF
                     APITVEIKGYVTEDRQKTCSLVFEWPTPEDTNDDVTVKVDESEGRILEV"
     gene            complement(568..1128)
                     /locus_tag="PKZ53_21715"
     CDS             complement(568..1128)
                     /locus_tag="PKZ53_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43116.1"
                     /translation="MTSGMITPSASLQVRSANPHWGRWIQVHLVIGVLLLAVGSGTGC
                     RTREPGVVTAVATSRQSATLWQPLEITQPVQFEHFQIPKGKYFVLPIIEIQDGNPSIV
                     EMLLSTQAGIAPHSVDRSTGKTSDGALGIFIPILGRATLRTQFANHQARFSFNPQIGT
                     IWFGLAHTDQEWCSMTLKRLSVSEPN"
     gene            complement(1140..>1650)
                     /locus_tag="PKZ53_21720"
     CDS             complement(1140..>1650)
                     /locus_tag="PKZ53_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43117.1"
                     /translation="SRVNSRCCFWSLPTGTTSAGNPPASSTSTGNPTAPTGPQSLWQP
                     VTVEKDCLLENVTISAGSYFVFPVIETVDGQKSITQMVLARKQDLKVEEIDKEKHTAG
                     DGTFAIFLPVVGRAELKTGFTENKARAALNPQIVSLWYGLNGGNHEWVSTPLKLKNPA
                     PAEPPGKKK"
BASE COUNT          408 a          392 c          427 g          423 t
ORIGIN      
        1 acttccagga tgcgcccttc tgactcatca accttcacgg tgacatcatc attggtatct
       61 tcaggtgttg gccattcaaa aaccagcgaa caggttttct gacgatcttc agtgacatac
      121 cccttgatct cgacggtgat gggcgcaaag agtttaaagc ctgatccggt gatataatca
      181 tagagatact tcataaacac caccgaatta aacaatacct catattcttc cagcttctcg
      241 ggtgtcggat ttggggcatt gactacaatc ttgttgggga cataggtttc attcccaacc
      301 cagataaacc gtttgcgtgc aagttcatcc aggagatgcc gtgcaattga gtttggggca
      361 atatcgttag gagtcagcca gtaattcgct ttgactgatt cggtcaaaaa ctgttccagg
      421 ttgatcaaca tttgtcgggt gtcgcttgcc ataaacgaat cgtccgttga gtgaagaggt
      481 caaaagttat actgccaggc agtggaggga agtgggtggg gcaatcccaa agagatgatt
      541 aagtcctctg agaaaacaca atgaggatca gttcggttca gaaaccgaga gtcgtttcaa
      601 agtcattgag caccattcct gatcggtatg agccagtcca aaccagatgg tgccaatctg
      661 cgggttaaag gaaaacctgg cctgatggtt ggcaaattga gtgcgaagtg tggctcgccc
      721 gaggattgga ataaaaatcc ccagtgcccc gtcactggtt ttcccggttg accgatcaac
      781 tgagtgaggg gcaatgccag cctgagttga gagaagcatt tcaacgatac tggggttgcc
      841 atcctggatt tcaataatcg gaagcacaaa gtacttcccc ttcggaatct gaaaatgttc
      901 aaactgtaca ggttgcgtaa tttcgagtgg ttgccagagt gtggctgatt ggcgggaagt
      961 tgccacggct gtaaccacgc ctggttctct ggtccgacac ccggttccgc tcccgaccgc
     1021 cagtaagaga accccaatta cgaggtgcac ttgaatccac cgaccccagt gtgggttggc
     1081 tgacctcact tgaagactgg cagatggggt gatcattcca gaggtcatgg atacacacct
     1141 tattttttct tcccaggtgg ttcagccggc gccggatttt tcagtttaag cggtgtcgag
     1201 acccactcgt gatttccacc attcaaccca taccataaag acacaatctg cgggttcagt
     1261 gcagctctgg ctttattttc agtaaacccg gttttgagtt ctgcccggcc aacgacggga
     1321 agaaaaatgg caaacgttcc atcgccggcg gtgtgctttt ctttgtcaat ctcttcaact
     1381 ttcaaatctt gtttgcgggc aaggaccatc tgagtaattg atttttgtcc gtcaacggtc
     1441 tcgatcactg gaaacacaaa gtatgatccg gcggaaatcg tcacattttc taaaagacaa
     1501 tctttttcaa ccgtgactgg ctgccagagg gattgcggtc cggttggcgc ggttggattc
     1561 ccggtcgaag ttgatgaagc gggtggattc ccagcggagg tggtgcccgt gggcaaggac
     1621 cagaagcagc acctcgagtt cacgcgcgat
//
LOCUS       DAOQDO010001678         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81303_length_1647_cov_2.323492, whole genome shotgun sequence.
ACCESSION   DAOQDO010001678 DAOQDO010000000
VERSION     DAOQDO010001678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81303_length_1647_cov_2.323492"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..301)
                     /locus_tag="PKZ53_21725"
     CDS             complement(20..301)
                     /locus_tag="PKZ53_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43118.1"
                     /translation="MTNLQQRVLAGLIALPLLFVSIWLGSPYFFCGLVLVTTLLGLHE
                     FFLLVEKIGFHPVRAAGFAMMLVIEGLFFLNQPGSDWFLITIGASQSLI"
     gene            complement(335..1129)
                     /locus_tag="PKZ53_21730"
     CDS             complement(335..1129)
                     /locus_tag="PKZ53_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNJ43119.1"
                     /translation="MFNFEGLIEPGSRDEALLSQLDPQRMPSHIAIIMDGNGRWAKRQ
                     GKPRVMGHPAGIESVREIVETCARLGITTLTLYTFSTENWRRPRFEIDMLWKLLRECL
                     HKEIDLLDRNNVQLRFIGRLHEIESSLLRDIQKAEQKLQHKTGLVLNIAFNYSGRAEL
                     TDAFRALATECQLTGRSPQSITEDDIARHLYIGSDPDLLIRTSGEMRLSNFLLWQLAY
                     TEIVITDTLWPDFRRPNLFESILAFQNRDRRFGGVEATNQKKPARV"
     gene            complement(1259..>1647)
                     /locus_tag="PKZ53_21735"
     CDS             complement(1259..>1647)
                     /locus_tag="PKZ53_21735"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNJ43120.1"
                     /translation="GRIISPFWHRYVVAVPTSINWEAMHMAAQSLVGTFDFTPFTVAD
                     CEVHTHTRTISDIAWRPLSDEVISLEFSGNGFLKYMVRRMVGLLMDVGTNRYPVSVVS
                     DVLAGRSSPQITTAPAKGLTLIRVDY"
BASE COUNT          424 a          410 c          441 g          372 t
ORIGIN      
        1 cggcagacaa aatgcagttt caaattaacg actgcgatgc gccgattgtg atcaagaacc
       61 agtctgagcc gggttgattg aggaaaaaga gcccttcaat cacgagcatc attgcaaacc
      121 ctgctgccct caccgggtga aatccaatct tctcaaccag aaggaaaaac tcatgcagcc
      181 cgagcagggt ggtgaccagt accaggccac agaaaaagta aggtgagcca agccagatgc
      241 tcacaaagag cagcgggagt gcgatcaaac cggcgagaac acgttgttgg agattggtca
      301 tgatgtaagg aaccgtggcc tttgattgat caggtcagac ccgtgctggt tttttttggt
      361 ttgtagcttc aaccccacca aaccggcgat ctcgattttg gaaggccaga atggattcaa
      421 acaggtttgg acggcgaaaa tcaggccaca gggtatcggt gatgactatc tcggtgtagg
      481 ccagttgcca gagtaaaaaa ttgctcagcc gcatctcccc actggtccga atcaataagt
      541 ctggatccga gccgatatac aaatgacggg cgatgtcgtc ttcggtgata ctttgtggcg
      601 agcgcccggt caactggcat tcagtggcga gtgcccggaa ggcatccgtg agttcggccc
      661 gaccactata attaaaggca atgttgagca cgagcccggt tttgtgctgt aacttctgtt
      721 ccgccttttg aatatcgcgt agcaagctgg actcaatttc atgcagccgg ccaataaatc
      781 gtagctggac gttgttgcga tcaagcaggt caatttcttt gtgcaggcat tcgcgcagca
      841 gtttccagag catgtcaatc tcaaaccgtg ggcgacgcca gttttccgtt gaaaaggtat
      901 agagcgtgag ggttgtaatt ccaagtcggg cacaggtttc aacaatttca cgcactgatt
      961 cgatccctgc cggatgaccc atgacccgag gttttccctg gcgtttggcc cagcgtccat
     1021 tgccatccat gatgatggca atgtgtgagg gcattcgctg cggatcaagc tgggagagca
     1081 acgcttcatc acgcgatcca ggttcaataa gtccctcaaa attaaacatg acaattctcg
     1141 atcccaaagc acgatgatcc ggtgagcccg gatggctgtg cctcaaggtt tggatgattg
     1201 gtttgatagg tccagcaagc gaggcaggaa aactcgaagt gtactaaagt ggccgatact
     1261 agtagtccac ccgaatcaga gtcaaccctt ttgctggggc ggttgtgatt tggggcgaac
     1321 ttcggcccgc cagtacgtcg gaaacaactg agaccgggta gcgattggtt ccaacatcca
     1381 tcaacaggcc aaccatacgc cggaccatat actttaaaaa cccattcccc gaaaattcca
     1441 gactgatcac ctcgtctgag agcgggcgcc aggcaatatc agaaatggtg cgggtatggg
     1501 tatgcacctc acagtcggcc accgtaaatg gagtaaagtc aaatgtgcca accagtgatt
     1561 gggccgccat atgcatcgct tcccagttga ttgaagtcgg aacggccacg acgtaccggt
     1621 gccagaatgg gctgatgatt cttcctt
//
LOCUS       DAOQDO010001679         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81476_length_1645_cov_2.954088, whole genome shotgun sequence.
ACCESSION   DAOQDO010001679 DAOQDO010000000
VERSION     DAOQDO010001679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81476_length_1645_cov_2.954088"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PKZ53_21740"
     CDS             <1..1050
                     /locus_tag="PKZ53_21740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNJ43121.1"
                     /translation="MSLGRLLPTSVVTRIVIDAAQGLHTIHQAGVVHRDVKPPNLFLS
                     GDGTTILGDFGIAVDTSASHEQPNIAGTLMFMAPEQALSSNLDRRSDIYSLGATGHYL
                     ATGKFVVEAHNLVELAKAQCHPYTPPPTDDPGKAYFFSVISRMLRHQPEDRYQTAETT
                     ARMLKVIAEPMPRYTLATSSEAVVGKIRLVLQVGDIATQEVGVIVNAANTMMLMRVGV
                     AGALHRAGGPEIEQEAQTQAPVPMGEVVWTKAGNLRAAWVAHAVAAIDGAICLQRCTL
                     RALLGAEARLQSSIAFPALGSGVGEVPMDLVAKLMLEAIRTFASLQLPSVRLIKIVLR
                     DQNALTRWQTILHAM"
     gene            1234..1473
                     /locus_tag="PKZ53_21745"
     CDS             1234..1473
                     /locus_tag="PKZ53_21745"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNJ43122.1"
                     /translation="MAVHIQFDVPVDMPTQEANQKAKELFLCSLYDEEKLSEKQVCDL
                     LGISRRDFQLLLTRYHVPRLRTLDDVNDEIFFAAQ"
BASE COUNT          405 a          433 c          409 g          398 t
ORIGIN      
        1 atgagtcttg gacggttgct tccaaccagc gttgtgaccc gaatcgtgat tgatgccgct
       61 cagggattgc acacgattca tcaggccggg gttgttcatc gggatgtgaa accaccaaac
      121 ctgtttttaa gtggcgacgg aacaaccatt ttgggagatt ttgggattgc ggttgacacc
      181 agtgcttccc acgaacagcc caatattgct ggaacactga tgtttatggc gcccgaacag
      241 gcgctgagca gtaatctgga tcggcggagt gatatttatt cgctcggcgc aaccggacat
      301 taccttgcca ccggcaaatt tgtcgttgaa gcccacaatc tggtcgaact ggccaaagcc
      361 caatgccatc cgtacacacc gcctccaacc gatgacccag gcaaagccta tttcttttca
      421 gttatttccc ggatgctcag gcatcaaccg gaggaccgct accagacggc tgaaaccacg
      481 gcccggatgc tcaaagtgat tgccgagcca atgccgcgct acacactggc cacctcatcg
      541 gaagccgttg tcggaaaaat tcggctggtc cttcaggtgg gcgatatcgc aacccaggaa
      601 gtcggcgtca tcgtcaatgc cgccaatacc atgatgttga tgcgcgttgg tgtcgccggc
      661 gccttgcacc gtgctggagg ccccgagatt gaacaggaag cacaaaccca ggctccagtt
      721 ccaatgggcg aagtggtctg gacgaaagcc gggaacctcc gtgcggcctg ggtcgctcac
      781 gcagtggcgg caattgacgg agcgatttgt ttgcaacgct gcacgctgcg ggcgttgctt
      841 ggtgccgaag cccggttgca atcttcaatc gcctttccag cactcggttc cggtgtgggc
      901 gaagttccaa tggacctcgt tgcgaaactg atgctcgaag ccattcgcac tttcgccagc
      961 ctgcaacttc catcggtacg attgatcaaa atcgtgcttc gtgaccagaa cgccctcacc
     1021 cgatggcaga cgattttgca cgccatgtag tgaacgaatc agcactcaac tcacggcttt
     1081 ttcgaagtta aaactctgta ctcaagagga aaccttcaaa catcgctcct ataatttttc
     1141 atagaactat ttgatctcaa aagacttgca cttactgact tttggatcta cattgagttc
     1201 acacaacctc caaaactgat agcggaatgg attatggcag tacatataca atttgatgta
     1261 ccagttgaca tgccaacaca ggaagccaat caaaaggcaa aagaactttt tctgtgtagc
     1321 ctgtatgacg aggaaaaact ttctgaaaag caggtgtgtg atcttttggg aatcagcagg
     1381 cgtgactttc aacttctttt aacccggtac cacgtgccac ggttacggac tctggatgat
     1441 gtcaatgacg agatcttttt tgctgcccaa tgacctacac gtacatcgtc agtgattcca
     1501 cacctctcat gcacctggaa gcccaactcg acggattttc ttttttgaga cgggtttttg
     1561 agaaagtcta tatccctcag gcagttgctg aggaactggc agaaggatat ggcatcacac
     1621 ctgaagagta tcttcagatg tatgc
//
LOCUS       DAOQDO010001680         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81491_length_1645_cov_2.673585, whole genome shotgun sequence.
ACCESSION   DAOQDO010001680 DAOQDO010000000
VERSION     DAOQDO010001680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81491_length_1645_cov_2.673585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..990
                     /locus_tag="PKZ53_21750"
     CDS             280..990
                     /locus_tag="PKZ53_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ43123.1"
                     /translation="MDSILLIDDDIELCQLMEEYLEPEGFLIEKVHTGRLGVERALAG
                     TYSLVVLDVMLPELNGFDVLRQIRARSKVPVLMLTARGEDVDRIVGLEIGADDYLSKP
                     FNPRELLARMRAILRRTRPEPTPTGLVLPTEKIQVNDVELDPRSRTVRRGGKLIELTT
                     VEFDLLDTLLRAAGNIVSREHLCETALGRSFHPFDRSIDMHISNLRKKLGARSGGQER
                     IKSIRGVGYIYTFESDEP"
     gene            1195..>1645
                     /locus_tag="PKZ53_21755"
     CDS             1195..>1645
                     /locus_tag="PKZ53_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43124.1"
                     /translation="MQSLFLKIFLWFWLAMALMVAALILATMTVDSSDRRFRTSFGRM
                     ISVQALSAVETYERSGAAGLTGFLERVNKISGLRGYFYDRDGALVSGIGADSPEKSGL
                     AQQVKTQRRVQVETLDTVMLVATPIFDSKGALFVYIEEIPRPPFNPWL"
BASE COUNT          378 a          384 c          433 g          450 t
ORIGIN      
        1 aattctatct ttaaaacaat caaagccagc aaaataagag gtgcttgttg ctggtactta
       61 taccattttg ggctgagggt tgaaggtaaa aggctgaagg ctaagggtat tgggctaagg
      121 gtaaagggta aagggtcagt ttcctctcat cccaaccttt tttaccatct cggaatgatc
      181 atatcgtatt atggagaaag ctaaattact gaaaaaattg tatttccctt agccctcaac
      241 cctcagccct caacccttag ccctcagccc tgcaaaccta tggactcaat tctactcatt
      301 gacgatgaca ttgaactatg ccagctcatg gaagaatacc ttgagcctga agggtttttg
      361 attgaaaaag tccacacggg ccgcctgggt gttgagcgag cgttggcggg aacctattca
      421 ctggttgtcc tggatgtgat gttgcctgaa ttgaacggtt ttgacgtcct tcgccagatt
      481 cgggcacgct caaaagtacc tgtcttgatg ctgaccgccc gtggggaaga cgtggatcga
      541 attgtcggcc tcgaaatcgg tgcggatgac tatctgtcca aaccatttaa tccacgcgaa
      601 ttactggctc ggatgcgggc tattttgcga cgtacccgcc cagaaccgac tccgactggt
      661 ctggtgttac cgacggaaaa aatccaggtc aatgacgtcg agcttgaccc ccggtcacgg
      721 actgtccgac gcggtggaaa actgatcgaa ctgaccacgg ttgaatttga tttgctcgat
      781 acattgctta gagcggctgg caatatcgtc tcacgtgaac atttatgcga aaccgcgtta
      841 ggccggtcat tccatccgtt tgaccgaagt attgatatgc atatcagcaa cctgcgcaaa
      901 aagctcggag cacgatctgg cggacaagag cggattaaat ctattcgggg cgtgggatat
      961 atctacacct ttgagagtga tgagccgtga atccgggttc cgggtcttcg gaagggatga
     1021 gggatgaggg atgagggatg aaagaaatca ccattcactt tgtcagttgg tcatcctgtc
     1081 agagttccgc gcttccggtt tccgggtctt cgaagtttga tccttttggt cctttttgtc
     1141 ccttatgtcc tttttgtgct tttcctaaac ccccgaactc ccgaaccctc aattatgcaa
     1201 agtctctttc tcaaaatttt tctctggttc tggctggcaa tggcgctgat ggtcgcggca
     1261 ttgattcttg ccacgatgac ggttgattcc tctgaccgcc ggtttcgcac ttcgtttggg
     1321 cgaatgattt cggttcaggc gttgagcgcg gttgaaacct atgagcggtc tggcgcggct
     1381 ggcctgactg gctttttgga acgagtcaat aaaatttcag gcttacgcgg atacttttat
     1441 gatcgggatg gggctttggt gagcgggatt ggggcggata gtcctgaaaa atctggcctg
     1501 gctcagcaag tgaaaactca gcggcgggtt caggtcgaaa cactggacac ggtgatgctg
     1561 gtggcgactc ccatttttga ttctaaaggg gccttgtttg tctacattga agaaatcccg
     1621 cgtccaccat tcaatccctg gcttg
//
LOCUS       DAOQDO010001681         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81495_length_1645_cov_2.623270, whole genome shotgun sequence.
ACCESSION   DAOQDO010001681 DAOQDO010000000
VERSION     DAOQDO010001681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81495_length_1645_cov_2.623270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKZ53_21760"
     CDS             <1..234
                     /locus_tag="PKZ53_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43125.1"
                     /translation="KVSEPDLWLSWLNRAIDAIRRPTATLPVVAGFKTHPYLMLITSG
                     DPTDLDRALNPQCFLASTELNLLNAVLFAEEAP"
     gene            complement(269..1594)
                     /locus_tag="PKZ53_21765"
     CDS             complement(269..1594)
                     /locus_tag="PKZ53_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43126.1"
                     /translation="MKELLRQKLIDSLAGQPPPRTLREVHLPDVPGKAIAVIGPRRGG
                     KTTFLWQILAHRLDQGTPREGLVYFNFEDERLAGLAATDLHLVVEEYFRLYPGWRDHK
                     KATFFFDEIQVVPGWELFAWRLLDTENIDLFLSGSSARLLSREVATSMRGRAMEALIF
                     PFSFREFLRHRNVEPQIPPDRLPKAERSAMDKELLTYLTTGGFPEAQGRHPSRDRFEL
                     LRGQVDTMLLRDVIERHAVSHPVALRWLVRQLLGSAGSSFSINKFYGDLRSQGIPVAK
                     DTLYAYVTHLEDAFLVLTVPIATDSERRRMVNPRKVYPIDPGLTPVFDRSGKANLGHS
                     LETAIALDLKRHGADIAYVRTRQDFEVDFLARAHDGTHALIQVSADLSDPVTRNRECR
                     ALIEASAEYPTATLNLISLTADHVPGVDHRIQLFSAANWLLQTDRIGSL"
BASE COUNT          395 a          421 c          453 g          376 t
ORIGIN      
        1 aaagtcagcg aacctgatct ttggttgtcc tggctcaatc gggcgattga tgcgattcgc
       61 cgacctacag cgaccctgcc ggtggtagcc gggttcaaga cccacccata tttgatgttg
      121 atcacatctg gggatccaac tgatttagat cgagccttga atcctcagtg ttttttagct
      181 tcaaccgaac tcaatttgtt aaatgctgtt ttatttgccg aggaagcccc ttgaacagag
      241 tcaaggattc ctcaaagaaa tctggccact acaaactacc gattctatct gtttgcaata
      301 accagttggc ggctgaaaaa agctgaatgc ggtggtccac tccgggtaca tgatccgccg
      361 ttaacgaaat cagattgagg gttgccgttg gatattcagc agaagcctca atcaaggccc
      421 gacattcgcg gtttcgagtg accgggtcag acaaatcagc actaacctgg atcaaggcgt
      481 gagtgccatc gtgagcgcgg gccaggaagt caacttcgaa gtcctgccgg gtgcggacat
      541 acgcgatatc cgctccgtgt cgtttcaggt caagggcaat ggcggtttcc agcgaatgcc
      601 caaggttggc tttcccagat cggtcaaaaa cgggagtcaa ccctggatca atcggataaa
      661 ctttgcgtgg attgaccatt cggcggcgtt cggaatcagt ggcaatcggc acggtcaata
      721 ccaggaaggc atcttcaagg tgtgtcacat aggcatagag ggtatctttg gcaactggaa
      781 ttccctgtga tcgaagatcg ccatagaatt tgttgatact aaacgagctg ccggcactgc
      841 caagtaattg ccgaaccagc cagcggagcg cgaccggatg cgagacggca tggcgctcga
      901 tgacatcgcg caacagcatg gtgtccacct gcccacgtag caattcaaat cggtctcgtg
      961 atgggtgccg gccctgggct tcagggaagc cgccggttgt gagataggtg aggagttctt
     1021 tatccatggc cgaccgttca gcttttggca ggcgatctgg aggtatttgc ggttcgacgt
     1081 tccgatgccg gagaaattca cggaaactga acggaaaaat cagagcttcc attgcgcggc
     1141 cacgcatact ggttgctacc tcacggctga gcaaccgggc tgacgagcca gaaagaaaca
     1201 ggtctatgtt ttcggtgtcg agcaatctcc aggcgaatag ttcccagcct ggcacaacct
     1261 ggatttcatc aaagaaaaac gtagccttct tgtggtcgcg ccagcctgga tagagccgga
     1321 aatattcttc taccaccaga tggaggtcag tcgcggccag acctgcgagg cgttcgtcct
     1381 caaagttgaa atacacgagt ccttcgcggg gggtgccctg atccagtcgg tgggcaagaa
     1441 tttgccacag gaaggttgtt ttaccgcccc gcctggggcc aatgacggca attgccttgc
     1501 ccggcacgtc agggaggtgc acctcccgaa gcgtgcgtgg tggtggctga ccagccagtg
     1561 aatcaatcaa tttctgtcgg agtaactctt tcatttctcc tcctcataag gaataaaggg
     1621 ctccccattg tctagacaaa aaaac
//
LOCUS       DAOQDO010001682         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81625_length_1644_cov_2.030837, whole genome shotgun sequence.
ACCESSION   DAOQDO010001682 DAOQDO010000000
VERSION     DAOQDO010001682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81625_length_1644_cov_2.030837"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1388)
                     /locus_tag="PKZ53_21770"
     CDS             complement(162..1388)
                     /locus_tag="PKZ53_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43127.1"
                     /translation="MFRPQPLGIFPFPTGSFILPDCSEAAGFAQFLLQGKIPPETPDR
                     LRFYSLALTGDFQGAFELLEQDVSIEGQFNHFVLQPTAELYARLKRTLPVEFLPLLDD
                     VAYSTGLISMPLNAPQLDGELLGFSYLIQATSQIEAGDIGAASSLLTFGINEAESVSP
                     VLAAQLRNSLAEMKLQIEGPTDEVLQLYIQALSVFEKTDLAEAQAGVHLNLGICLQDR
                     SQGNRAVLVEAIKHYQSALRFFTFDAFPEAFALAQNNLALAYLAMPMQEASDQLRMGV
                     AIQALREVLKVYTRATHPERWASAQLNLANALQYLPSTHTQENLIEAVNLYEEIVSVR
                     RAETDPLGFARLLANQGNCLAHLGIFDHASQKLQAARDLFATNGEPESAATVSELLAE
                     IDAHQPSSVKVEATVG"
BASE COUNT          409 a          399 c          448 g          388 t
ORIGIN      
        1 aattgttgtc gttggtaaag gtgcatagtg ctgagggttc tgggttctgg gttccgggtt
       61 ccgggttccg ggttccgggt tccgggtttt cgagaaaaag acaaaaagga cataagggac
      121 aaaaaggaca taaattcgaa tacccggaac ccgaagtcgt cctatcccac ggtggcttcc
      181 actttcacag atgacggttg gtgggcgtca atttcagcta gcaattcaga gacagtggcc
      241 gccgattcag gttctccgtt ggttgcaaac aggtcgcgag cagcctgaag tttctggctg
      301 gcgtgatcaa atattcctaa atgtgccaga caatttccct gattggccag aagccgggca
      361 aacccgagcg gatcggtttc tgctcgacgc accgacacaa tttcctcata taaattgacg
      421 gcttcgatca aattttcctg ggtatgggtt gagggcaggt attggagcgc gttggcaaga
      481 tttaattgag cgctggccca gcgttcaggg tgggttgccc gggtatagac ttttaacact
      541 tcgcgcaggg cctggattgc cacacccatc cggagctggt cgctggcttc ctgcattggc
      601 atcgccaggt aggccagggc cagattgttt tgggccaggg caaaggcttc ggggaaagca
      661 tcgaacgtga aaaatcgaag cgctgattga taatgcttga tggcttcaac cagcacggcg
      721 cgattgcctt gcgaccgatc ctgcaggcaa atccccagat tcaaatggac cccggcctgg
      781 gcttcagcca ggtcagtttt ttcaaagacg gaaagcgcct gaatgtagag ctgaagcacc
      841 tcatcggttg gtccttcgat ctgaagcttc atctctgcca gactattgcg gagttgtgcc
      901 gccagaaccg gagaaaccga ttcagcctca tttatgccaa aggtcaatag tgaactcgca
      961 gcgccaatgt ctccggcttc gatctgactg gtagcctgaa tcaaatagga aaagccaagt
     1021 aactcaccat ccagttgagg tgcgttcaag ggcatcgaga tcaacccggt actgtaggcc
     1081 acatcatcca gcaacggaag aaactcaaca ggtaacgtcc ttttcagcct ggcatagagt
     1141 tccgccgttg gctgtaaaac aaagtgattg aactgacctt cgattgaaac atcctgttcc
     1201 agtaattcaa atgcaccctg aaaatcaccc gtcagtgcga gcgagtaaaa ccgcaaccga
     1261 tctggagttt caggcggaat cttcccttga agcaaaaatt gagcaaaccc agccgcttca
     1321 gagcagtcag gcaatatgaa actgccggtc ggaaatggaa aaatgccgag tggttgtggt
     1381 cgaaacatgg gaaaaccttt tttggatcag ggttcggggg ttttcagaag ggataaggga
     1441 tgagggatga gggatgaaag aaatcacccg gtcaccttgt cattcggtca tctggtctgg
     1501 gttctgggtt atcaaacttt ggtctggtcg tccttttgtc tttcaggccg tcaacgacct
     1561 gcctcaattc agccaccgct acgcggcaaa aaccaggata caatgaccgg gtgaacaggc
     1621 gagggtttca tccttcatct cttc
//
LOCUS       DAOQDO010001683         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81693_length_1643_cov_2.323048, whole genome shotgun sequence.
ACCESSION   DAOQDO010001683 DAOQDO010000000
VERSION     DAOQDO010001683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81693_length_1643_cov_2.323048"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..>1643)
                     /locus_tag="PKZ53_21775"
     CDS             complement(267..>1643)
                     /locus_tag="PKZ53_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43128.1"
                     /translation="HVYFNVLEKDNQFWKLAANQENLERLQTALGWVRHPQVRLVLEA
                     GIISMRARMEKPFTAWEQWLVLKKENPAHPAGYLSFKDENEIEELEESFEDMETFLKS
                     PNIEIADNKGFLDDDLAADWREVFVPKLVARQKEKLTPVESLHLAVVLCHLGVEPEKN
                     LAQVVALAGQSREMAREALAALDEIEVDKSAQLEFFVQFLQREPEKCDELAQELHQLA
                     SYNLPDMHHPSVAGRLIEVYLQTLESDVQHRILEAILSIKPVIPFEQLMRLQLSDEIG
                     EMIWGQYVAALAESDPERARTVLRKWLLTRPKQKFLLLKQLALLGDPLIREPVIKELE
                     VTRDDHARRELVSIIRAFGDPALLLQLQFNSRLEPWMETTLLDLIPHGASDPRLFNWV
                     EAQFQNANLNRRRELLMPYLRAFQKLRDRRALPSLYSMLEIGQHQADIAKVIGEIVLD
                     EALLQDRP"
BASE COUNT          407 a          425 c          381 g          430 t
ORIGIN      
        1 aaggctgttg atggtatgtg ctttccggcc tggcacctga accaggtgct gtatttgtgc
       61 ggcgtgaatt tcatcagata tacactgttt cctttgtaga gtaggtttgg ttgaattgcg
      121 gtctggcgat aaccgtgggc tggtaaggac cttaccgaaa atttccagac attttgaacc
      181 acgacataca cgaaaaaaac cagacacttc caaaattcaa catctcagga aactgttgac
      241 aatgccaatt agagacacga gctgggtcag gggcgatcct ggaggagcgc ttcatccaac
      301 acgatttccc caatcacttt ggcaatatct gcctggtgtt gaccgatttc caacatggaa
      361 tacagggatg gaagcgcccg ccggtcacga agcttttgga atgcacgcag gtatggcatc
      421 aataattcgc gccgtcggtt cagattagcg ttctgaaatt gggcttccac ccagttgaat
      481 aaacgcgggt cagatgcccc atgcgggata agatccagca aggttgtttc catccagggt
      541 tccagcctgg agttgaactg caactgcagc aacagggctg gatccccaaa tgctctgata
      601 atcgagacca gttcccggcg ggcatgatca tcgcgggtga cttcaagttc ttttatgacc
      661 ggctcgcgga tgaggggatc acctaataaa gccagttgct tgagcagaag aaacttttgc
      721 tttggccgag taagcaacca tttccgaagc accgtccgcg cccgttcagg atcgctttca
      781 gcaagcgctg caacatactg tccccagatc atttccccaa tttcatctga gagctgcaat
      841 ctcatcagtt gttcaaatgg aataaccggc ttgatgctca atattgcttc caaaattcga
      901 tgctggacgt cactttccaa ggtttggaga tagacttcaa tcagccgccc agccactgat
      961 ggatggtgca tgtctggtag attgtaactg gcaagctggt gtaattcctg ggccagttca
     1021 tcgcattttt caggttcccg ctgaagaaac tggacgaaaa attccaactg ggctgactta
     1081 tctacttcga tttcatccag ggcggccaat gcttcccgtg ccatttcccg cgattggcca
     1141 gccagggcaa ccacctgagc cagatttttt tccggctcga cgcccaaatg acacaacacc
     1201 accgctaaat ggagcgactc aacgggtgtg agcttctctt tctggcgagc aaccagcttt
     1261 ggaacgaaaa cttctcgcca atcggctgca aggtcatcat ctaaaaaccc tttattgtca
     1321 gcaatttcga tatttggcga tttgaggaac gtttccatat cctcaaatga ttcctccaac
     1381 tcttcgattt cattttcatc tttgaaactc agatagcctg ccgggtgcgc cggattttct
     1441 tttttcagca ccaaccattg ttcccaggca gtaaatggtt tttccatccg tgcccgcata
     1501 ctgataatgc cagcttcaag caccagccga acctgtgggt gacgcaccca accaagcgcc
     1561 gtctgaagcc gttccaaatt ttcttgattt gcagccagct tccagaattg attgtctttt
     1621 tcaagcacat tgaaatagac atg
//
LOCUS       DAOQDO010001684         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81739_length_1642_cov_3.966604, whole genome shotgun sequence.
ACCESSION   DAOQDO010001684 DAOQDO010000000
VERSION     DAOQDO010001684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81739_length_1642_cov_3.966604"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1205
                     /locus_tag="PKZ53_21780"
     CDS             99..1205
                     /locus_tag="PKZ53_21780"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNJ43129.1"
                     /translation="MLLAFVAWLLMDALIVLMCWYMPVSRAEEAFFGVRVSSEFYRDH
                     GHRLLLQYRLWLGLTFLQIEVLGALVTIFRGEIGLARIIGVFLLLFANSALYVVFARQ
                     IKPFALVEENQRYAAPLITRHLADYTNLAVEIAILIALIGPFLALVYFYPQLPEWIPV
                     HWNWKGEVDDWARKSFTSVFAIPVLTLYMHGLLFLVKRGLLGVKMTLPGEHAAEYLEL
                     KERQLNLNLNLMDWMRALTSVVLGAIALNTVFSSLPEFASYARLVNIVLFSITGVMFV
                     IGFWYLFQMIGVNRDLKTRVGRVYVQRPSDAEHWYGGGTIYYNPEDPAFFVEKLVGWG
                     YTVNFAHREAQFLMGYTLLMPVLALWLIFGSKSN"
     gene            1404..>1642
                     /locus_tag="PKZ53_21785"
     CDS             1404..>1642
                     /locus_tag="PKZ53_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43130.1"
                     /translation="MCCFARRSFILAISLVFLLGCLLFTTQSGKYTPFNLISPPLKAA
                     PISHTLPEASFNGVKRQVITATVTGGGASCNGAPVA"
BASE COUNT          345 a          415 c          415 g          467 t
ORIGIN      
        1 cttggttctt ggaaggtatt ggttccaaag cactaagccc caagcacgaa gccccaagta
       61 ccaggcccca agcccttcgc cctgaaatga ggtggattat gttgctcgca tttgtagcct
      121 ggctcttgat ggatgcgttg atcgtgctga tgtgctggta catgccagtt tcacgggctg
      181 aggaagcctt tttcggtgtg cgtgtgagtt ctgaatttta tcgcgaccac ggtcatcggt
      241 tgctgctcca gtaccggctc tggttggggc tgacattttt gcaaattgaa gtgttgggcg
      301 ccctggtcac gatctttcgc ggagagatcg ggttggcccg gattattggg gtgttcctgc
      361 tcctctttgc caattcagcc ctgtacgttg tgtttgcccg tcaaatcaaa ccctttgcgc
      421 tggtggagga gaatcaacgg tatgcggcgc cgctcattac ccgtcatctg gctgattaca
      481 ccaatctggc ggttgaaatc gcgattctca ttgcgttgat tggacccttt ctggcgctgg
      541 tgtatttcta tccgcaactc ccagaatgga ttccagttca ctggaactgg aaaggcgaag
      601 tggacgactg ggcgcgcaag agttttacct cagtctttgc cattccagtg ctgacactgt
      661 atatgcatgg acttctcttt ttggtcaaac ggggactgct cggagtcaaa atgacccttc
      721 ctggtgagca tgcagcagaa tatctggaac tcaaagaacg ccaactcaac ctgaacctca
      781 acttgatgga ctggatgcga gcattgacct cggttgtgct tggtgccatc gcgctcaata
      841 ccgtgtttag cagtttgccc gaatttgcct catatgcccg cctggtcaat attgtgttat
      901 tttcgatcac gggtgttatg tttgtaatcg gtttctggta tttatttcaa atgattggag
      961 taaatcgaga tttaaagact cgggtcggtc gggtttatgt ccaacgtcca tctgatgccg
     1021 aacactggta cggcggcggg accatctatt acaacccgga agacccggcg ttttttgtcg
     1081 aaaaactcgt cggatggggc tacacggtga acttcgccca tcgtgaagcc cagttcctga
     1141 tgggatatac cctcctgatg ccggttctgg ccctctggct tatttttggg tcgaaatcca
     1201 attagagaag tgcaagaacc agggctttcc ggcaacggaa agcccattta ccttgaatca
     1261 acctctccca ggtggttcaa tattcactcc ctgccagttc atctcttcag gccaaaaatc
     1321 aacccgcaaa aattcagcac ttacgatcct ccctgtacct gctgaaccag tcaggttttg
     1381 cattgcttat ccaaggagaa cctatgtgtt gttttgccag gcgatcattt attttggcta
     1441 tttctctggt gtttctgctc ggatgtcttt tgtttacaac tcaaagcgga aaatacaccc
     1501 cctttaattt gatatcacct cccctcaaag cggcaccgat ttcgcacacg ttgcctgagg
     1561 caagttttaa tggtgtcaag cggcaggtca tcaccgccac ggttactggc ggaggcgctt
     1621 cgtgcaatgg agccccggtg gc
//
LOCUS       DAOQDO010001685         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81743_length_1642_cov_3.780718, whole genome shotgun sequence.
ACCESSION   DAOQDO010001685 DAOQDO010000000
VERSION     DAOQDO010001685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81743_length_1642_cov_3.780718"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1587
                     /locus_tag="PKZ53_21790"
     CDS             226..1587
                     /locus_tag="PKZ53_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNJ43131.1"
                     /translation="MTTPQRELLPLEASALFEQKIVELMKKAEGKRKPVKAIRLIPHP
                     LARALKNCGTAHIYTDTADQNEMYDLLVQSEDDQNFFLYEDVDGNTTNQPLVVKVLNR
                     FFEGDPDDTLVAWIEQLQQLKPELTLEQATILIYSIINGRLGCEVQDFYSAGRTWEIS
                     LELHTSLAPTPEASKQIGWCLNRAVPNAFVKVPFTPHYPHSLLIARDLENQGIPVNFT
                     ATFSARQIVAAVLLANPHRTNVFLGRLSQGLGSELLGEQVVLATQRYVKQLRDQFGCR
                     TLNMLASVRRWQTMVLTAGCDVYTVPYPVLKEFLNQQLVSPDQLENCVDKDYADQLTI
                     EPAVLDRVGLEKIQKLYQVEPEFIEFLTKLRASAEFQTIDGDQLYKRFDEAGFGDFFY
                     VPSATEWKELRKSKLPDLESPLTKALPLDTLYSLLAIGDFINCQDHMDGLIQSPIRHL
                     FQG"
BASE COUNT          417 a          414 c          407 g          404 t
ORIGIN      
        1 aagggattat tccagaaatt cagccatttt cattcaattg acggctttgg cacgacaatt
       61 gtctaaggga tatttgcccc tcaaccaagc actcgctggg tttttttaag cactttcatc
      121 gaaatatccc cacagtgttt ggctttttgc cagtcacaca cctgtgtctg gcttcaagtt
      181 ctgaggcttg ccccacacat cttcacacag cagcaggaaa atgccatgac gacaccgcaa
      241 cgcgaattgt taccgcttga ggcttcggct ttgtttgagc agaaaattgt tgagttgatg
      301 aaaaaagctg aaggcaaacg caaacccgtc aaagcaattc gattgattcc gcatccgctg
      361 gctcgcgccc tcaaaaattg cggcacggca cacatttaca cggatacggc ggatcaaaat
      421 gaaatgtatg atctgctcgt ccagtcagaa gacgatcaga atttcttttt gtatgaggac
      481 gttgatggaa acaccaccaa ccagcccctg gtggtcaaag tcctgaaccg gttttttgaa
      541 ggtgacccgg acgatacact ggtggcctgg atcgaacaat tacaacaact caaacctgaa
      601 ctgacgcttg agcaggccac catcctgatc tatagcatta tcaatggacg gctcgggtgt
      661 gaagttcagg atttttatag tgcaggccga acctgggaaa tcagcctgga gctgcatact
      721 tcgctggcac ccaccccgga agcgtccaaa caaatcggct ggtgcttgaa ccgtgcggtt
      781 ccaaatgctt ttgtcaaagt tccatttacg cctcactatc cgcattcact tctgattgcg
      841 cgggaccttg agaatcaagg aattccagtc aacttcaccg cgacattttc ggctcgccaa
      901 attgtcgcag ccgtgctgct ggccaacccc catcggacca atgtgttcct cgggcgatta
      961 agccagggac tgggttctga actcctcggt gaacaggtgg tactggccac tcagcgctat
     1021 gtcaaacagt tgcgcgatca gtttggctgt cgaacgctca atatgctggc cagtgtcaga
     1081 cgctggcaaa ccatggtttt aacggctgga tgtgatgtgt acaccgtgcc gtatccggtg
     1141 ctgaaagagt ttctcaacca gcaactggtg tcgccagatc aattggaaaa ttgcgttgat
     1201 aaagactatg cggatcagct caccattgag ccagcagtgc tcgaccgcgt agggctggaa
     1261 aaaatccaga aattgtatca ggtggagcct gaatttattg agttcttaac caaactgaga
     1321 gcctctgccg aatttcagac aattgacggt gatcaattgt acaaacgatt tgatgaagcc
     1381 ggattcggtg actttttcta tgttccgtcg gcaactgaat ggaaggaact gcgcaaaagc
     1441 aagcttcccg acctggaatc accgctgacg aaggcgctcc cactcgacac gctctatagc
     1501 ctgctggcga ttggggactt catcaactgc caggaccaca tggatggact gattcagtcg
     1561 ccaatccggc atttgtttca gggctgaaga cttcaggttt ggggctgaag acttcgggct
     1621 gaagaagtcg ggctgaaggc tt
//
LOCUS       DAOQDO010001686         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81777_length_1642_cov_2.424701, whole genome shotgun sequence.
ACCESSION   DAOQDO010001686 DAOQDO010000000
VERSION     DAOQDO010001686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81777_length_1642_cov_2.424701"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKZ53_21795"
     CDS             <1..522
                     /locus_tag="PKZ53_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43132.1"
                     /translation="AVQPVGRLWIWFGFSIGGIAFLTFRQLFELPLRYFVNANFLLIG
                     ISVLWEWLVLPLKDQSAAFSLFYLQTAVLLWLTAPLLLGILSCCFPAAWWWRAILLMS
                     VLGYAFLFSAVKYVMCLWALTWIGTPILLNLYCFLGPLLDVVLVSGFFAVFLSWVSNT
                     MNQSLNQEKIWQW"
     gene            516..1607
                     /locus_tag="PKZ53_21800"
     CDS             516..1607
                     /locus_tag="PKZ53_21800"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ43133.1"
                     /translation="MVAILQLWVLLVVGVAIVYTLRNWMLTLHRLFRPQRLYYQDILD
                     SDLPAISILIPMHNEEVVAAQILDTLLRSDYPRNRLEIIPINDHSSDQTAKILDEYAR
                     QFEFIQPLHRHTGQRGKPSGLNEALARAAHEVVLVFDADYQPSRGLLRSLAVAFLDPE
                     VGAVMGRVIPKNVSAGILTRLLDFERSAGYQIDQQARYSLDLIPQYGGTVGGFRKSVI
                     QKLGGFDPRMLAEDTHLTFQLYLAGWRGAYANRAECYEEVPETWEVRYRQLRRWARGH
                     NQVFFRYAWPLVTSSNLTLWQKTDGLLVLAMYALPVLMLSGFLVNLVLFLLGTKPVAR
                     RTRPPPRASMRSCCCSLNPSICLRPASST"
BASE COUNT          310 a          451 c          433 g          448 t
ORIGIN      
        1 gcagtccaac cggttggacg gctgtggatc tggttcggtt tttcaattgg tggaattgcg
       61 tttcttacct tccgtcagct ctttgagcta ccgcttcgct attttgtgaa tgcaaatttt
      121 ttgctcattg gaatctcagt gctctgggaa tggctggttc tgccgctcaa agatcaatcc
      181 gccgccttca gtttatttta tcttcaaacc gcggttttgc tgtggctgac cgcaccgttg
      241 ttactgggta tcctgagttg ttgttttccg gctgcctggt ggtggcgtgc aatcctgctg
      301 atgagtgttt tgggatatgc gtttctcttc tcagccgtga aatatgtgat gtgcctgtgg
      361 gcactgacct ggataggaac gccgatcctg ctcaatcttt actgcttttt gggtccctta
      421 cttgacgtgg tgctggtctc cgggttcttt gccgtctttt tgtcgtgggt gtcgaataca
      481 atgaatcaat ccctgaacca ggagaaaatc tggcaatggt agccatactc cagctatggg
      541 tgctcctggt ggttggggtg gcgattgtct acacccttcg caactggatg ctgacgctgc
      601 accggctgtt tcgcccacaa cgcctctatt atcaagatat tctcgatagc gatttgccag
      661 ccatttcgat cttgatccca atgcacaacg aagaagttgt ggcagctcaa atcctggaca
      721 ccctgcttcg gagcgactat ccgcgcaacc gacttgaaat cattccgatc aacgatcact
      781 catcagacca gacagccaaa atcctggacg agtacgcccg ccagttcgaa tttattcaac
      841 cgctccatcg ccatactggc cagcgcggca aaccttccgg cttaaacgaa gccctggctc
      901 gcgccgcgca tgaagtcgtg cttgtgtttg atgccgatta tcaacccagc cgggggctcc
      961 tgcgcagcct ggcagttgcc tttttggatc cagaagttgg tgcggtgatg ggccgcgtga
     1021 ttcccaaaaa tgtttcagcc ggaatcctca ctcgactgct tgattttgaa cgctccgccg
     1081 gctatcagat tgaccagcaa gcccgatatt cgcttgattt aatccctcaa tatggcggca
     1141 cggttggagg atttcgtaaa tcagtcattc aaaaactggg tggatttgac ccgcgaatgc
     1201 tggccgaaga tacccacctc acctttcaac tctatctggc tggctggcgg ggggcctatg
     1261 ccaatcgggc cgaatgttat gaagaagtcc ccgaaacctg ggaagttcgt tatcggcaac
     1321 tccgtcgctg ggcgcgtggc cataatcagg tcttttttcg gtatgcctgg ccgctcgtca
     1381 catcatcaaa cctgaccctc tggcaaaaaa cggacggctt gctggttctg gccatgtatg
     1441 ccctcccggt tttgatgttg agcggctttc tggtcaacct ggttctgttc ctgcttggca
     1501 ccaaaccggt tgcgcgacgc acgcggccgc cgccacgggc gtcgatgagg tcgtgctgct
     1561 gctctctcaa tccctccatc tgcttgcggc cggcgtcgag cacgtaggac agcagcatgg
     1621 ggtcgtcgac cggcgcgatc gt
//
LOCUS       DAOQDO010001687         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81893_length_1641_cov_2.381463, whole genome shotgun sequence.
ACCESSION   DAOQDO010001687 DAOQDO010000000
VERSION     DAOQDO010001687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81893_length_1641_cov_2.381463"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PKZ53_21805"
     CDS             complement(<1..1613)
                     /locus_tag="PKZ53_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43134.1"
                     /translation="MKAQSSTPELTDSQTDWMHPSKLEEALLKYDLTLLANQLAAKSL
                     ASDSPTELAFRIRVYIRAGHFSRLEQALPEIQRIKNYTDLFRDALTDLIDFGEIRLAQ
                     KLIELMPGLTPQTESVARFFDLMVENSSLQETDAWLAARVKLEPAWFEHHFDFVLHHG
                     SVEPLLAQLRLNVEQHPEDWFRAYEYTDYARKAGRVDSFDWMATVCRPVLAVDCYRFA
                     TLVEGTAAKRSFLEMALNRPFTPVDKDRMKSGYSIIPKDVDWEQLFEATVKKELAEGY
                     LLDKQPEKAQQLMEEAARLTPRGIPLQDLGATAGMTQLVSGKRVIEQSILENEAAYRK
                     EPQYWIERAHYYYGRDEYKLVDETIEKALAQFPLNKKNIENRNRQQIAYLSFWWLEKS
                     ESKGKMNPQLNRWIKEFESAPADHGYAIFLFRQLKPYLKDPDKYWVFPNGRMWHILAL
                     GSTWESNEPDEIEDQLKKARPDQQEVICLLLERIAKDGDVSKQYGVCAALCQAGLYKR
                     AIPLLKRLMKTSPPGSGYAHRLLWDCYLNT"
BASE COUNT          398 a          406 c          384 g          453 t
ORIGIN      
        1 gtgttgagat aacaatccca aagcagtcgg tgagcatacc cgctaccagg aggactcgtt
       61 ttcatcagac gtttgagcaa ggggattgcc cgtttataga gcccggcctg acacaatgcc
      121 gcacaaaccc catactgttt ggagacgtca ccatctttgg cgatgcgctc cagaagtaaa
      181 cagattactt cctgctgatc tggtcgggct tttttgagct ggtcttcaat ttcatcaggt
      241 tcattggact cccaggtgga gcctaaggcc aatatgtgcc acattcgtcc gttgggaaaa
      301 acccaatatt tatctggatc tttcaaatac ggctttaatt gtcgaaacag aaagatggca
      361 tagccatgat cagccggagc tgattcaaat tccttaatcc accggtttaa ttgagggttc
      421 atctttcctt tcgattcaga tttttcgagc caccaaaagg aaaggtaggc aatctgctgc
      481 cggtttcggt tctcgatgtt ttttttattc aagggaaact gagccagtgc tttttcaatg
      541 gtttcatcca ccaatttgta ctcatcccgt ccgtagtaat agtgagcccg ctcaatccag
      601 tattgaggtt cttttcgata cgcagcttca ttctccaaaa ttgattgttc gatgacccgt
      661 tttccagaaa caagctgagt catgccagcc gtggccccca ggtcttgaag tggaattccc
      721 cttggcgtga gccgggccgc ttcctccata agctgctggg ctttttcagg ctgtttatcc
      781 agcaagtacc cttctgccag ctcttttttg actgttgcct caaacaattg ctcccaatca
      841 acatcttttg ggatgattga atagcctgat ttcatccgat ctttatccac gggtgtaaac
      901 gggcgattca acgccatttc taagaaggat cgctttgccg ctgtgccctc gactaaggtt
      961 gcaaacctat aacaatcaac tgccagtact ggtcggcaca cggttgccat ccagtcgaag
     1021 gaatccaccc ttcctgcttt tcgagcgtag tcggtgtatt cgtaagcgcg aaaccaatcc
     1081 tcaggatgtt gctcgacgtt aaggcgcaat tgggccagca gtggttcaac tgatccatgg
     1141 tgcaatacaa aatcaaagtg atgctcaaac caggctggct ccaatttcac cctggcggcc
     1201 aaccaggcat cagtttcctg gagtgaactg ttctccacca tgagatcaaa aaatcgagct
     1261 actgattcag tctgtggggt taaacccggc ataagttcga tcaatttttg agcgagtcgg
     1321 atctcaccaa aatcaatcaa atcagttaag gcatcacgaa acaaatctgt gtaatttttg
     1381 attcgctgta tttccggtaa tgcctgttcg agtcgggaaa aatgtcccgc ccgaatatac
     1441 acccgaatac gaaacgccag ttcagttggg gagtcagagg ccagagattt tgccgccagt
     1501 tggttggcca gcagggtcaa atcatatttg aggagcgcct cttcaagttt cgaggggtgc
     1561 atccagtcgg tttgcgagtc ggtcagttca ggcgtggagg attgcgcctt caccagttgc
     1621 gaccctattg cattgggtag a
//
LOCUS       DAOQDO010001688         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_81989_length_1640_cov_2.403785, whole genome shotgun sequence.
ACCESSION   DAOQDO010001688 DAOQDO010000000
VERSION     DAOQDO010001688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81989_length_1640_cov_2.403785"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKZ53_21810"
     CDS             <1..456
                     /locus_tag="PKZ53_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HNJ43135.1"
                     /translation="VKRQTPAWLARLRNAGWQVTEFSVAEVIETFLKNDPRRKLWLAV
                     DRKSPQDWAKTNQALANALTTSGILEKKLEQLLADLDGDPNALVLVTDLEALHPYLRI
                     GAIESRLQGKFQVPTVFLYPGVRTGKTQLKFLGFYPEDGNYRSVHVGGA"
     gene            578..>1640
                     /locus_tag="PKZ53_21815"
     CDS             578..>1640
                     /locus_tag="PKZ53_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HNJ43136.1"
                     /translation="MNIRNLFDPGKGIDRIIEKVITYGVSQEKRLKAEISEYVVTEHI
                     EDQFRKLLDRMQLAIETKGGNEIGVWVSGFYGSGKSSFTKYLGFAFDDRVKIDGIPFL
                     KYLQDRLHSPQVKAHLGTVTHRYPAAVVMLDLASDMLAGATMEDISSVLYFKVLQWAG
                     YSRNLKVAALERRLEKDQRYAEFCARIESDFPGLTWKEMQNDPLVVDGLVPQIAHELY
                     PTLFPTPASFSSSTEGFIQFENQRVQEMIDIVREKSSKQHIIFIVDEVGQYIATRDNL
                     ILNLDGLAKNLKRLGDGKVWIIATAQQTLTEDDPRARLNSDKLYKLKDRFPIQIDLES
                     SDIKEICYRRLLGKSPAGED"
BASE COUNT          405 a          440 c          423 g          372 t
ORIGIN      
        1 gtgaaacgac agaccccggc ctggctggcc agacttcgca atgcgggctg gcaggtcact
       61 gagttttcag tggctgaggt gattgagaca ttcctcaaaa acgacccccg ccgtaaactc
      121 tggctggctg ttgatcgcaa atcacctcaa gactgggcca agacaaacca ggccctggcc
      181 aatgccttga ccaccagcgg gatccttgag aaaaagctgg agcaactcct ggccgatctc
      241 gatggtgatc ccaacgctct ggtgctggta accgatctgg aagccctcca cccgtacttg
      301 cgaattgggg ccattgagag tcggcttcaa ggaaaattcc aggtgcccac cgtttttctt
      361 tatcctgggg tccgcaccgg gaaaacccaa ttgaaattcc ttggtttcta tcccgaagat
      421 ggaaactacc gctcggtcca cgtgggcggt gcttagtttt ttgccagctc aaggcaaatt
      481 cctcattgag ccgcagttgc agattcaaac tccccaatcc gctgcacatt gatgctttgt
      541 cagtggtctg atttcacttc aaacggaaac aggtgctatg aacattcgca atttgtttga
      601 tcccggcaag ggaattgacc gcatcatcga aaaagtcatc acctatggcg tgtcccaaga
      661 aaagcgcctc aaggccgaaa tttcagaata tgtcgtcacc gaacacattg aggaccaatt
      721 tcggaagttg ctggatcgaa tgcaactggc tatcgaaacc aagggaggaa acgaaatcgg
      781 cgtctgggta tcagggtttt atggctcggg aaaaagttcg ttcaccaagt accttgggtt
      841 tgcctttgac gaccgggtga aaattgatgg aattccattc ctcaaatacc tccaggatcg
      901 gctgcattcg cctcaggtca aggcccatct gggaactgtc acacaccggt atcccgccgc
      961 tgtcgtgatg cttgacctgg ccagcgacat gctcgccggg gccacgatgg aggacatttc
     1021 ctccgtgctc tatttcaagg ttttgcaatg ggccgggtat tcgcgaaacc tcaaggtggc
     1081 tgccctcgaa cggcggcttg aaaaagatca gcgctatgcg gagttttgcg ccaggattga
     1141 atctgatttt ccagggctga cctggaaaga aatgcagaat gacccgctgg tggtggatgg
     1201 tctcgttcca cagattgccc acgagttgta cccgacgctg tttccaacac ccgcttcgtt
     1261 ttcgtcgagc accgaaggat tcattcaatt cgaaaaccag cgtgtccagg aaatgattga
     1321 tattgttcgc gaaaagagca gcaaacagca catcattttc atcgtggacg aagtcggcca
     1381 gtacattgcc acccgtgata acctgattct caacctcgat ggtttggcga aaaacctcaa
     1441 acggctgggg gatgggaaag tctggattat cgccacggcc cagcagacct tgaccgaaga
     1501 cgacccccgc gcccgcctga actcagacaa actatacaag ctcaaagacc ggttcccgat
     1561 tcagattgac cttgagtcga gcgacatcaa ggaaatttgc taccgccgct tgttgggcaa
     1621 atctccggca ggcgaagacg
//
LOCUS       DAOQDO010001689         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82036_length_1639_cov_3.767677, whole genome shotgun sequence.
ACCESSION   DAOQDO010001689 DAOQDO010000000
VERSION     DAOQDO010001689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82036_length_1639_cov_3.767677"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PKZ53_21820"
     CDS             <1..1608
                     /locus_tag="PKZ53_21820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HNJ43137.1"
                     /translation="QNQTQLQLKRALNQSPQVVAQAKLGAALAERPQVTNLPIQRFQD
                     PETKLEVNIEELSLERATHYLLLNGRGQLPLTFEEYIRLTKRELELLKEKQKSYPVEP
                     PNPVLASKVNLLTDEEISQLSQSEATNFLLLANRGVSIEPAIYMKLLQRELTLMKARV
                     SGEESSGLAPMTVTTTEDVRTPAVRREETAETARRSAIETLMRAAKQLRETISQREVQ
                     EHFLKVFKHSSPFETVLSNLTRMAEYLQSTRLRIDYRDGDPSSANAFTLSGVITLRTR
                     FFDKLDPAEQRNTVIHEAVHAALGTPDYAYTTERLITALKPDIAVRNPDSYVWFLAGL
                     LEGKKEPKNQVQDTFTGEFSEFQKFELAKTIGIATSSFIRVQELFGQAAEDANQAYKV
                     PRKFEHNTIAAIQQVKKVFTGPSEKWNQDIPTEGIAKGLTLVSQMLTYLYGGLTVPVN
                     ITWTDDGTPKWTRVGTGPVSLQVPRGISGENLYKSLMRNYVKDLLEPTRGEKWKQYYN
                     LIVNVSPTFINLGNYIGTSMSPLTPEM"
BASE COUNT          496 a          400 c          370 g          373 t
ORIGIN      
        1 caaaaccaga cccagttgca actcaagcgg gcactcaatc agagcccaca ggtggttgcc
       61 caggcaaaac tgggcgctgc tctggcagaa cgacctcagg ttaccaacct gccgattcaa
      121 agatttcagg atccggaaac taagctggaa gtcaacattg aagaactctc actggaacgc
      181 gcaacgcatt atcttctcct gaatgggcga ggtcagcttc cacttacatt tgaggaatac
      241 atccgactga ccaaacgaga gcttgaactc ctgaaggaaa aacagaaaag ctatccggtt
      301 gagcctccca atccagtttt agccagcaag gtcaatttgc tgactgatga agaaattagt
      361 cagttatccc aatctgaagc gacaaatttt ttgctcctgg ccaatcgagg ggtctcaatt
      421 gaacctgcga tttatatgaa actgcttcaa cgggaactca ccctgatgaa agcccgcgtt
      481 tcaggagaag aatcgagtgg cttagcccct atgacagtta cgacgactga ggatgtgaga
      541 accccagccg tccgccggga agaaacggct gaaaccgcca ggcggagcgc catcgaaact
      601 ctgatgcgtg ccgccaagca gttgagagaa acaatcagtc aaagggaagt acaggaacat
      661 ttcctcaaag tgtttaaaca tagctctcca tttgaaacag ttctctcaaa tcttactcgt
      721 atggctgaat acctgcagtc cacgagactt cgaattgatt accgtgacgg agacccatca
      781 tcggccaatg cctttacgct ttcaggagtg attacactca ggacacgatt cttcgacaag
      841 ttagacccag cagaacagcg aaatacggtc attcacgagg ctgtccacgc ggcgttggga
      901 acacctgact atgcctacac aaccgaacga ctgattacgg ccttaaaacc agatattgca
      961 gttcgtaacc cagacagtta tgtctggttc ttagccgggt tgcttgaagg aaaaaaagag
     1021 ccgaaaaatc aagtccagga tacatttaca ggtgaatttt cagaatttca gaagtttgaa
     1081 ctcgctaaaa ccattggtat tgcaacctct tcgtttatac gggttcaaga attattcggc
     1141 caggcggctg aagatgcaaa ccaggcgtac aaagtccctc gcaaatttga acacaacacc
     1201 attgccgcca ttcaacaggt aaaaaaagta tttaccggac catccgaaaa atggaaccag
     1261 gatattccga ccgaaggcat tgccaagggg ctaacccttg tttcccagat gctgacatat
     1321 ctttatggag gcttaactgt acctgtgaat attacctgga ccgatgatgg aacacccaaa
     1381 tggacccgcg ttgggactgg cccggtgagt ttacaggtcc caagaggcat ttcgggtgaa
     1441 aatttatata aatcattgat gcgaaactat gttaaagatc tacttgagcc aaccagagga
     1501 gaaaaatgga agcagtatta caacttgatt gtcaacgttt cccctacgtt catcaatttg
     1561 ggaaattaca ttgggacgtc tatgtcgcct ctgacacctg aaatgtaact ggactctttc
     1621 gccaaagaag gccaaaacc
//
LOCUS       DAOQDO010001690         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82281_length_1637_cov_2.453856, whole genome shotgun sequence.
ACCESSION   DAOQDO010001690 DAOQDO010000000
VERSION     DAOQDO010001690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82281_length_1637_cov_2.453856"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..>1637
                     /locus_tag="PKZ53_21825"
     CDS             244..>1637
                     /locus_tag="PKZ53_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ43138.1"
                     /translation="MRIPDPEPGTRNPEPGTPKSMDYPNIYSLFRNRVEQFRGQQVFY
                     TRQDDQWVGSTWDQFDEDVHNFASALIASGLTPQRSVCILMKTVPEWPVADLGTIAAG
                     GISVGLYPTNSAEQCQYIINHSDAEFVLVDTRGQLEKVLSVRDQLPHVKAIICLSESA
                     AQAHAGVISYRTFLEFGKSRRLETAGELETRAEGATADDTAIMVYTSGTTGLPKGACL
                     SHRYILHSAKSLEGSIPIQSDDTSFSYLPYCHVAERISGLYNRLNAGAAAYFVDDLTK
                     LWDYMLEVKPTVFGSLPRFFEKIYARILGDVHKLPPEEQARFQQAREVGKQVSQFRQR
                     GESVPADLQAQYDELVEPFAQKIKSYFGGRIRIATSGGAPLPQEIAEFFSGFGLPLLQ
                     AYGLTENICVAFNRPERYKFGTVGPAMEGCEIRIADDGEILVRSQMQFSGYYKQPEET
                     ADVLRDGWVYTGDLG"
BASE COUNT          369 a          391 c          434 g          443 t
ORIGIN      
        1 ttccgggttc cgggttccgg gttccgggtt ccgggttccg ggttttcgaa accagcacat
       61 attctgtcat ttgtcttctg atcggttggt tttggtaagt gtttgattct tttcgtgttt
      121 tttgtgtttt tcgtggttaa gaatgtcttg gagttcttga tagcggactt ctttgctttt
      181 gaaccacagg cccagccagt actgattcaa tagcttacaa ttccttcaat ttgatcattt
      241 attttgcgaa taccagaccc ggaacccgga acccggaacc cggaacccgg aacccccaaa
      301 tctatggact atcccaacat ctattctttg tttcgcaatc gagttgagca atttcgtggt
      361 caacaggtgt tttacacgcg tcaggacgac cagtgggttg gcagcacctg ggatcagttt
      421 gacgaggatg ttcacaattt tgcttcggca ttgattgcga gcgggctgac cccccagcgt
      481 tcggtgtgca ttttgatgaa gaccgttcca gagtggccag tggccgatct gggcacgatt
      541 gctgccggag gcatcagcgt tgggctctat ccgaccaatt cagccgaaca atgccagtac
      601 atcatcaacc attcggatgc tgaatttgtc ctggttgata ctcgtggtca gcttgaaaaa
      661 gtgctctcgg ttcgtgacca gcttccgcat gtcaaagcaa ttatttgcct ttcagaatct
      721 gccgctcagg cgcatgcggg agtaatcagc tatcgtacat ttcttgagtt tggaaaatcc
      781 cgtcgtttgg aaaccgccgg agaattagaa accagggccg agggcgccac tgctgatgac
      841 acggcaatta tggtttacac ctcaggtaca actggtttgc cgaaaggtgc ctgcctgagc
      901 catcggtaca ttttgcacag tgccaaatcg ctcgaaggct cgatcccgat tcaatcagat
      961 gacacatcgt tttcctattt gccctattgc catgttgctg agcggatttc aggactctat
     1021 aaccggctca atgccggggc agcggcttac tttgtagatg atttgacaaa gttgtgggac
     1081 tacatgctcg aagtcaaacc aactgtgttt ggcagcctgc cgcgcttttt tgaaaaaatc
     1141 tatgcccgca ttttgggaga tgttcataag cttccgccag aggagcaggc tagattccag
     1201 caggcacgtg aggtcggaaa acaggtcagt cagtttcgac agaggggtga atcggttccg
     1261 gcagacttac aggctcagta tgatgaactg gtcgaaccat ttgcccaaaa aatcaaaagt
     1321 tattttggag gccgcatccg gattgccact tcaggtggag cgccgttgcc tcaggaaatt
     1381 gctgagtttt tcagcgggtt cggattgcca ctgctccagg catatggcct gaccgaaaat
     1441 atctgtgtcg cctttaaccg gcctgaacgc tataagtttg gaacggttgg cccggcaatg
     1501 gaaggctgtg aaatcagaat tgccgacgat ggggaaattc tggttcgaag ccagatgcag
     1561 ttcagcggat attacaagca acccgaagaa accgccgacg tgctccggga tgggtgggtg
     1621 tacactggcg accttgg
//
LOCUS       DAOQDO010001691         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82343_length_1636_cov_3.420620, whole genome shotgun sequence.
ACCESSION   DAOQDO010001691 DAOQDO010000000
VERSION     DAOQDO010001691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82343_length_1636_cov_3.420620"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1636)
                     /locus_tag="PKZ53_21830"
     CDS             complement(<1..>1636)
                     /locus_tag="PKZ53_21830"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ43139.1"
                     /translation="RTVKLPASTRSSTTPQTPKPKPKDPVIEVDYSQPRLVVQLGHTM
                     DVDSVTFSEDGSLAVTGSWMEGTAIVWDVQSGREIRRFTGNTDNKGYQDGWISGAISP
                     DKRLVVVLSEFSFRLWDLVTGKLVKRIRITDGDYIESGEFSFSPDQTRLIWHNNISRK
                     VWDLATGKIIENKRGKGEPDLRPEAYLSPDGKLKAVIEEKVVRIMDTATGAEVSRLDG
                     NPSVVARTTITPAGLQVATVQGNAVFVRDAATGRAVEVGTSAYEILRPDAKISPDGRL
                     LAATSRGNVGIWDIQTGHQLLTLTGHTDDVSSVAYSPDSKYLVTNSFDFTVRMFDLAT
                     GKERLQLKKTSNLETPIAFTPDCTQIAYAIEPDESGKLFVYVQGLMSRRVLKRLLVTT
                     DGNTWVHDIALSPDKKQMVVATGSMDDATLFDVQVWDLETEKLVRHLKGHSTYVQAVA
                     YSADGKTIATNGWDTTIRLWNAATGAQLRVLKGHVGPVGAVEFFANDQLLLSQSFDST
                     SRVWNVQTGAELACLTSFETGDWLVLTPDGRFDTNNP"
BASE COUNT          369 a          436 c          419 g          412 t
ORIGIN      
        1 ggggttgttg gtatcaaagc gtccatcagg ggttaatacc agccaatcgc cggtttcaaa
       61 tgaggtcaaa cacgcaagtt ccgcgccggt ctggacattc cagactcggg aagtactgtc
      121 aaagctttgt gaaagcaata actggtcatt ggcaaaaaac tcgaccgcgc caaccggccc
      181 cacgtgacct ttcaagaccc gcaattgggc tccggtcgcg gcattccaca agcggatggt
      241 tgtgtcccaa ccattggtgg caatggtctt gccatcggct gagtaggcaa ccgcctggac
      301 ataggttgaa tggcctttga ggtgccgaac cagcttttcg gtttcgagat cccacacctg
      361 gacatcaaac aacgtggcat catccattga gccagtggca acgaccatct gttttttgtc
      421 tggagacagc gcaatatcgt gaacccaggt attgccatcc gtggtcacaa gtagcctttt
      481 cagcacccgg cgagacatca aaccctgcac atagacaaac agtttgccgc tttcgtcggg
      541 ctcaattgcg taggcaatct gggtacaatc cggtgtaaaa gcaattggtg tttccaaatt
      601 cgaagttttc ttgagctgca ggcgctcttt gccggttgca aggtcaaaca tccgcacggt
      661 gaaatcaaaa ctgttggtaa ccagatattt gctgtcaggt gaataggcaa cggatgaaac
      721 atcatcggtg tggccagtca aggtcagcag ttggtgtccg gtctggatat cccagattcc
      781 gacattgcca cgactggttg cggccaggag tcgcccatcc ggcgaaattt tggcatctgg
      841 tcgcaagatt tcataagccg aagtgcccac ttcgacagct cgcccggtgg cggcatctcg
      901 tacaaataca gcgtttcctt gaacggttgc cacttgtagc cccgctgggg tgatggttgt
      961 tctggcgacg acacttggat tcccatccag gcggctgact tctgcgccag tggctgtatc
     1021 cataatccga accacttttt cctcaatcac cgctttgagt tttccatcag gtgacagata
     1081 ggcttctggg cggaggtctg gttcaccttt cccacgttta ttttcaatga ttttcccggt
     1141 tgccaggtcc cacactttgc gtgaaatatt gttgtgccag atgagccggg tttgatcggg
     1201 cgaaaacgaa aattcaccac tttcaatata atcgccatcg gtaattcgaa tccgtttgac
     1261 gagttttccc gtgaccagat cccacaaccg aaaactgaat tcgctgagga ccacgaccag
     1321 ccgtttatcg ggtgaaattg caccgctgat ccagccatcc tgatagcctt tgttgtcggt
     1381 gttgccggta aagcgccgga tttcgcgccc gctttgaaca tcccacacaa tggcggtgcc
     1441 ttccatccag cttccagtca ccgccagcga accgtcttcc gaaaaggtca ctgaatcaac
     1501 atccatggta tgcccaagtt gcacgaccag gcgcggttga ctgtaatcaa cttcaatcac
     1561 tggatctttc ggtttgggct ttggcgtctg gggtgtggtt gatgatctgg tcgaagctgg
     1621 caatttcacg gtgcgt
//
LOCUS       DAOQDO010001692         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82370_length_1636_cov_2.552182, whole genome shotgun sequence.
ACCESSION   DAOQDO010001692 DAOQDO010000000
VERSION     DAOQDO010001692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82370_length_1636_cov_2.552182"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1636)
                     /locus_tag="PKZ53_21835"
     CDS             complement(<1..>1636)
                     /locus_tag="PKZ53_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNJ43140.1"
                     /translation="EVPKTTPPTVEEIPALQGLELDMKVLAELSGTELVKRLAPVTGA
                     YRQWIHDQAARIDDPAANLEEFRETARLALENCSRALTRIQEGITLLQTDPVAAEAFR
                     FANQAMYRQRVHSLYAERKRQGKEVTLSEVDIPANHRWFPFQMAFMLLNLPGLTQLDH
                     PDRTGEAQATADLLWFPTGGGKTEAYLGLTAYVLGLRRLQGEIEGRTGDYGVAVLMRY
                     TLRLLTLQQFQRASALICACESIRREAIEAKNTQWGQEPFRIGLWVGQKTTPNHTEDS
                     KAYVDRSQFQSGQSSSSTPHQLTNCPWCGEKINPGKDIKVEPYPNGRARTFIFCGDKL
                     GRCLFSSRRSPDEGIPILVVDEEIYRRLPALLIATVDKFAQMPWEGRTQMLFGQVDSY
                     CPRHGFRSPEVNDSSLHPAKGGMPAVKAVPIRPLRPPDLIIQDELHLISGPLGTLVGL
                     YETAVDRLASWEVNGKRVYPKVVASTATIRQAREQVHGVFLRQVQIFPPQGLDAADNF
                     FSRQRPPCETTPGRVYLGFCAPGKRLKIALIRTYIAL"
BASE COUNT          340 a          457 c          465 g          374 t
ORIGIN      
        1 agggcgatgt aggtccgaat caaggcaatc ttcaaccgtt tgcctggcgc gcaaaagcca
       61 aggtacaccc gccccggtgt tgtttcacag ggtggacgct gccgggaaaa gaagttgtct
      121 gccgcatcca gtccctgggg tggaaaaatc tggacctgtc gcagaaacac tccgtggacc
      181 tgttcgcggg cctgtcggat ggtcgcagtg gaggccacga ctttcggata gacccgtttc
      241 ccgttgacct cccaggaagc cagccgatca accgccgttt catacaatcc gacgagcgtt
      301 ccgagtgggc cgctgatcag gtgcaattca tcctggataa tcagatccgg tggccgcaaa
      361 ggtcgaatgg gtactgcttt gacagccggc attccacctt tagccggatg caaactgcta
      421 tcgttgactt ccggcgagcg gaagccatgg cggggacaat agctgtcaac ctgtccaaag
      481 agcatttggg tccggccttc ccagggcatc tgggcaaact tatccacagt ggcaatcagg
      541 agggccggga gtcggcggta gatttcttca tccaccacca ggattgggat tccttcgtcc
      601 ggtgaccgtc gagatgaaaa caggcagcgg cccagtttat cgccacaaaa gataaacgtt
      661 cgtgcccgac cattggggta gggctcaact ttgatgtctt tgccggggtt gattttctcg
      721 ccgcaccacg gacagttggt caactggtga ggggtactgg aactggattg acctgattga
      781 aactgacttc gatcaacata ggctttgctg tcctccgtat gattcggggt tgttttttgt
      841 cctacccaga gcccaatccg aaacggttcc tgcccccatt gggtattttt ggcttcgatt
      901 gcctcgcgtc gaatggattc acaggcgcaa atcaatgccg aggctcgctg gaattgctgg
      961 agtgtgagca accgaagcgt gtaccgcatc agaacagcaa ccccatagtc accagttcgg
     1021 ccttcgattt cgccttggag tcggcgcagg cccagcacat aggctgtcaa acccagatag
     1081 gcttcggttt tccccccgcc ggttggaaac cacagcaaat cagccgttgc ctgggcttct
     1141 ccggtgcgat ctggatggtc aagctgggtg agaccgggaa ggttcagcaa catgaacgcc
     1201 atctgaaatg ggaaccaccg gtgattggcc ggaatgtcca cctcggacag cgtgacttct
     1261 ttcccctggc gttttcgttc ggcatagagg gaatgaaccc gttgtcggta catcgcctga
     1321 ttggcaaatc gaaatgcctc tgccgcgacc gggtcggttt gtaacagagt aatcccttct
     1381 tgaatgcggg tcagcgcacg ggagcaattc tcaagggcaa gccgggctgt ttcgcgaaat
     1441 tcttccaggt tggcggctgg atcatcaatg cgcgcggctt ggtcgtgaat ccactggcgg
     1501 taggccccag tcaccggcgc cagccgtttc accagctccg tcccggataa ttctgccagc
     1561 accttcatgt ccagttccaa tccctgcaac gccgggattt cctcaaccgt cggcggtgtt
     1621 gttttaggaa cttcat
//
LOCUS       DAOQDO010001693         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82371_length_1636_cov_2.532574, whole genome shotgun sequence.
ACCESSION   DAOQDO010001693 DAOQDO010000000
VERSION     DAOQDO010001693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82371_length_1636_cov_2.532574"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKZ53_21840"
     CDS             complement(<1..234)
                     /locus_tag="PKZ53_21840"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43141.1"
                     /translation="MRRLSCTRHWLQLSMGLLFLVAGGLAHPEVVFGQEKGWVDRAPN
                     QEARQEVAVTELQGRIYLIGGIANRIVSNTVERY"
     gene            861..1199
                     /locus_tag="PKZ53_21845"
     CDS             861..1199
                     /locus_tag="PKZ53_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43142.1"
                     /translation="MKPKADVQYFEFLKYSKISWLTVAVILTIFGLVIANMMFNQPQT
                     TQEIASVKAEDFFAQQAAHQRSSSVNTLASHKANDAEYLRFLSISGVSPKRNLKTVSE
                     GNREERTQAN"
     gene            complement(1445..>1636)
                     /locus_tag="PKZ53_21850"
     CDS             complement(1445..>1636)
                     /locus_tag="PKZ53_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43143.1"
                     /translation="AGRMPAFPGGNLFLKNYSQLSRQEVRLTFGNRGMTQDLNRQQTE
                     PGQYQKTSMQQFVCQDREK"
BASE COUNT          408 a          411 c          351 g          466 t
ORIGIN      
        1 atatcgttca accgtattgc tgacgatgcg attggcgatg cctccaatta aataaatacg
       61 accttgcaat tccgtaacgg cgacttcctg gcgtgcctcc tggtttgggg cccgatcaac
      121 ccaacctttt tcctgaccaa aaacgacctc agggtgtgcc agcccaccgg caaccaggaa
      181 cagcaggccc atacttagtt gaagccagtg cctggtgcaa gacagtcttc tcattctcaa
      241 actcctgacg aaagccacaa atccaatgaa gcgtgtttct ccttgcactg gcggcccaat
      301 ttctcattga tatttcaacc ctgtgtctaa accacaaatt aaagcggctg acaaccccaa
      361 actgcattta tgggtgactt gaaacctgtg acttcccatc tctgcgtatc gttcaggcca
      421 gaaggtgtga cggcaccgtt ggcctgggta ttacctggct atttatttga tagaattgga
      481 tatggggaat ctctaaatca agtttttcca aacttttgaa tataaactat ttagcctgat
      541 ttttatggtt gttcggaatt gaaacacggg tttcccaaaa acgggcacct gcttgtgatc
      601 cccgcctttt tggcctggtg ttgatgactt attcccagga ggaaatagaa atttttactt
      661 ctgtaatttg ggcgatgggt tggcatgcaa tcatctgaaa ccgttttagt tagttttgat
      721 tttcttcttg tttcccttgg aaattcaggg atttttttta caagtaacac tgattttttt
      781 acacgtggca cacgattcgc cttttaaaag actgtcaaac gtcacgtaaa tggttcagcc
      841 acagaaagtt ctccaaaatt atgaaaccta aagctgatgt gcagtatttt gaatttttga
      901 agtattcaaa gatctcctgg ctaacagtcg ctgtgattct gacaatcttt ggcctggtaa
      961 ttgcaaatat gatgttcaat cagccgcaaa ccactcaaga gattgcttca gtgaaagccg
     1021 aggatttttt tgcccaacag gctgcccatc agcggtcatc atctgtaaat acactggctt
     1081 cgcataaagc caatgacgcc gagtatttgc gatttctatc aatctcggga gtcagtccaa
     1141 agcggaattt gaaaacggtt tctgagggaa acagggagga gcgaactcaa gccaattgat
     1201 ggtgagaaag aaccgacgcc agagtgagga gacgatcacc ggtggttctt tcaaaatcac
     1261 aaccaataac atccatcaat gacagaaagc ccttaacccc tcaacccata tggcgctcgt
     1321 cacggtcggc atcgtagtgt cggcctggtt gcgatcctgg ccgtgacgcg cgaccttata
     1381 ccacctttcc tgatttcttt cgctgcccac agttataccc tcgcatccct ctccggtttc
     1441 tgcctcattt ttccctatcc tggcaaacaa attgctgcat gctggttttc tggtattgcc
     1501 caggttctgt ctgctggcgg ttcagatcct gtgtcatccc ccgatttcca aacgtgagcc
     1561 gcacctcctg tcggcttaac tggctatagt ttttcagaaa aagattcccc cctgggaacg
     1621 ccggcatcct gccggc
//
LOCUS       DAOQDO010001694         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82449_length_1635_cov_2.997468, whole genome shotgun sequence.
ACCESSION   DAOQDO010001694 DAOQDO010000000
VERSION     DAOQDO010001694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82449_length_1635_cov_2.997468"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1187)
                     /locus_tag="PKZ53_21855"
     CDS             complement(138..1187)
                     /locus_tag="PKZ53_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNJ43144.1"
                     /translation="MSASMVSQPTDLKERWAEFRKNNPKVRIRDAAEQLGVSEAQLVA
                     TGCGETVTRLEGTWSDVLDAISPIGRVMALTRNEHAVHEKKGLYQGLETTGHVGLVHT
                     EGIDLRIFFSRWKLGFVVSEDSGQAFMRHSLQFFDQAGTALHKIFLQPESNFAALDEL
                     VKRFRSNDQSPVQTVEPETTSKTIRPDADIDVRGLQEAWYTLKDTHDFFGMLRTFEVE
                     RTQAFRLVGADLARPVSTQSARQVLTLASEQGVSIMVFVGNPGIIQIHTGPVSKIVPY
                     GDWINVMDPDFNLHLRETGIASAWIVRKPTLDGDVTSLELFDQEGNNIALFFGERQPG
                     KPELQSWRDLLADVK"
     gene            complement(1156..>1635)
                     /locus_tag="PKZ53_21860"
     CDS             complement(1156..>1635)
                     /locus_tag="PKZ53_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43145.1"
                     /translation="RYTTLSGGEQQRTHFARVLAQLWPAIGTVPVLLMLDEPTASLDL
                     QHQHHVLSTARHLTQLGVAVVVVVHDLNLAAPYADNIVLFQKGAVVASGTPVQVLTKP
                     ILERTFGIMVEVSSHPTRNIPLIVPTGADQPPLQFQPLSEKFVKGVRYECFNGIAAD"
BASE COUNT          401 a          421 c          429 g          384 t
ORIGIN      
        1 agtcaatttt cggagggaag gagaatcatt cataatgcat ttaccttagt taacagtgtt
       61 aactaaggta aatgcatgat tgaattttgt caatcaaaac ttttagaagc ggtaaagtac
      121 cagacggtcc aagactgcta tttcacatct gccagcaggt cgcgccacga ttgtaattct
      181 ggcttgccag gctggcgttc gccaaaaaac aacgcgatgt tgttgccctc ctggtcaaag
      241 agttcaagcg aggtcacgtc tccgtcaagc gtgggtttgc ggacaatcca ggcgctggcg
      301 attccggttt cacgcaggtg caggttgaaa tcaggatcca tgacattgat ccagtcgcca
      361 tagggtacaa ttttggaaac cgggcctgtg tgaatctgga taattcccgg attaccgaca
      421 aataccataa ttgagactcc ctgttctgaa gccagggtca gcacctgtcg ggcactctgg
      481 gtcgaaactg gtctggccag atcagccccg accagtcgaa acgcctgggt tcgttcgact
      541 tcaaacgtgc ggagcatgcc aaagaaatcg tgggtgtctt ttaaggtgta ccaggcttcc
      601 tgcagtcccc gcacatcaat atccgcgtct ggacgaatgg ttttggacgt ggtttctggt
      661 tcaaccgtct gcaccggtga ctgatcgtta gatcggaacc gtttcacgag ttcatcgagt
      721 gcggcaaagt ttgattcggg ctgcaggaaa atcttgtgaa gcgccgtccc agcctgatca
      781 aaaaactgaa ggctgtggcg cataaatgcc tgtccggaat cttcagacac cacaaatccc
      841 agcttccagc gtgaaaaaaa gattcgcagg tcaatgcctt cggtatgaac cagtccaacg
      901 tgcccggtgg tttccagacc ctggtagagc ccttttttct cgtgaaccgc gtgttcattg
      961 cgggtcaggg ccatcacccg accaatgggc gagattgcat ccagaacatc actccaggta
     1021 ccttccaggc gggtgacggt ttctccgcag ccggtggcca ccagttgtgc ctcactgacg
     1081 ccaagttgtt ccgccgcatc acggatccga acctttgggt tattttttcg aaattccgcc
     1141 caccgttctt tgagatcagt cggctgcgat accattgaag cactcatatc gaactccttt
     1201 aacaaatttt tcagacagag gttgaaactg aagcggtggt tggtcagcac cagttggcac
     1261 aatcaacgga atattgcgag ttggatggga cgaaacctcg accataattc caaacgtgcg
     1321 ctcaagtatt ggcttcgtca agacctggac gggtgttccg gatgcgacca ccgctccttt
     1381 ttgaaataaa acaatattgt cagcatacgg cgcggccaga ttcagatcgt gaaccacgac
     1441 caccacggca acgccaagct gagtcaggtg gcgggccgtc gaaagcacat gatgctgatg
     1501 ctgcaaatcg aggctggcgg ttggctcatc cagcataagc aaaacgggca cggtgccgat
     1561 ggctggccag agttgcgcca aaacacgggc aaaatgggtt cgctgctgtt caccaccaga
     1621 aagcgtcgtg tagcg
//
LOCUS       DAOQDO010001695         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82576_length_1634_cov_2.478151, whole genome shotgun sequence.
ACCESSION   DAOQDO010001695 DAOQDO010000000
VERSION     DAOQDO010001695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82576_length_1634_cov_2.478151"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..595)
                     /locus_tag="PKZ53_21865"
     CDS             complement(146..595)
                     /locus_tag="PKZ53_21865"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ43146.1"
                     /translation="MKAGWIGLILCSGILVTVQAAINAQLRTYVVNPFYSSLISFVVG
                     TVALLVIALISNATGQTGNWRGATQAPSWLLIGGVLGAIYVTSAILAIPKIGAGSVAT
                     SLIVGQLLAALMLDHFGWIGLPRFELTGSRIFGAGLLILGLWFIQRK"
     gene            650..907
                     /locus_tag="PKZ53_21870"
     CDS             650..907
                     /locus_tag="PKZ53_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43147.1"
                     /translation="MNAASSRCTLNAGNCLVMGELNARMRLWYCRSLELGLKTSGLGL
                     KIRKLRTEEERENSPMSSALSPMSSAQCLQPDVFKAQFKPD"
     gene            929..1357
                     /locus_tag="PKZ53_21875"
     CDS             929..1357
                     /locus_tag="PKZ53_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43148.1"
                     /translation="MNDLNDEKNTTDPTPDSVVKAESPKSSGGDEPRRPFDELTSSVR
                     ELAQKLPDSIGRIPGSIGAAIQSALSPRDQSVLVYINDDLSKSLDMLVTAEIFGSRSE
                     AAEFLMKEGLKAQQELFGRIQDKVQQIERLKSELKTIVPH"
BASE COUNT          447 a          389 c          441 g          357 t
ORIGIN      
        1 aaagatgcgg gctattgtag ggggcaacct ggattaaggc aaaggaagat tggcggaaga
       61 ttggaaaagt acggcaagcg gcctgttctg tgcagtcaga tggtggatga atttgtgttg
      121 aagacagggt gacaacgttc tgaattcatt tgcgttgaat aaaccataag ccaagaatga
      181 gcaatccagc cccaaatatc cgtgacccgg ttaattcaaa gcggggaagc ccaatccatc
      241 caaaatgatc cagcatcaat gccgccagta actgtccaac aatgagcgag gtcgccactg
      301 agcccgctcc aattttggga attgccagaa tggcagatgt gacgtaaatg gcaccgagca
      361 ccccgccaat caagagccag gatggggcct gagtggcgcc tcgccagttc ccggtttgtc
      421 ccgtagcatt tgagatcagg gcaattacca gtaaagccac cgtcccgacg acaaaactga
      481 tcaaagaact gtagaacggg ttgaccacat aggttcgcaa ctgggcattg atcgccgcct
      541 gcaccgttac caggattcca gaacacaaaa tcagtccaat ccaaccagct ttcactgtac
      601 cttctcactt tcaatgtgga gtgcggcggc ttgacgccgc tttggtatta tgaacgccgc
      661 gtcaagccgc tgcacgctca atgcgggaaa ttgcctggtg atgggtgaat tgaacgcacg
      721 aatgcggctg tggtactgtc gcagtttgga gctggggctg aagacatcgg gcttggggct
      781 gaagattcgc aagctcagga ctgaagaaga gcgggaaaac agcccaatgt cttcagccct
      841 cagcccgatg tcttcagccc aatgtcttca gcccgatgtc ttcaaagctc aattcaaacc
      901 tgattagttt ttgaaagagg tgaggtttat gaacgatcta aatgacgaaa aaaatacgac
      961 tgacccaact ccggattcgg tggtcaaagc tgaatctccg aaaagctcag ggggtgacga
     1021 accccggcga ccatttgatg aattgacttc gtctgtccgt gagctggccc agaagctccc
     1081 agattccatt ggccggattc cgggttcaat cggcgccgcc attcaaagtg cactctcccc
     1141 acgcgatcaa tcagtgctgg tgtatatcaa tgatgatctg tcaaagtcgc tggatatgct
     1201 tgtgacggct gagatttttg gcagtcggtc ggaggcggct gaatttctga tgaaggaagg
     1261 tctcaaagcc cagcaggagt tatttgggcg gatccaggat aaagtgcagc agattgagcg
     1321 gttgaaatca gaactcaaaa ccatcgtgcc gcactaaaga ttgcgcaccg gaattcgacg
     1381 ggtggttgtc aaaatagcgt ttgacgctga aaaaacgatt tggtagcgta cggctgcaca
     1441 aaacacactt aaacgaatcc tgattaaaac gcagtccact tcacacgacg gtcaaaccag
     1501 accccagccg ggaaggtctg acctttggca agcggtttgt tgttggaggc aagaggaaaa
     1561 aatgtcttac gaagcacttg gaatgattga aaccaaaggg tttattggcg cagtcgaagc
     1621 atctgacgcc atgg
//
LOCUS       DAOQDO010001696         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82579_length_1634_cov_2.462951, whole genome shotgun sequence.
ACCESSION   DAOQDO010001696 DAOQDO010000000
VERSION     DAOQDO010001696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82579_length_1634_cov_2.462951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKZ53_21880"
     CDS             complement(<1..200)
                     /locus_tag="PKZ53_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43149.1"
                     /translation="MSAEHSEQQFEAFCEDLEAYVEGALSDVRRREIESFLKDSAEAR
                     ELLAEFQQANSLFAAPEWEVPEP"
     gene            complement(255..821)
                     /locus_tag="PKZ53_21885"
     CDS             complement(255..821)
                     /locus_tag="PKZ53_21885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ43150.1"
                     /translation="MSKTDEELMRDFQRGDEAAFAVLYNRYRVLIYRFLARKISSPTR
                     AEDLTQEVFMAVVQHAREWREEASVKTYFYRIAFNRAVSELRRSEHKVMVAEQDHDSE
                     DGPPPKEAVSTDSPAQDYETRERARLVREALKEIDPDFRDAILLKEYEELKYEEIAEI
                     LNVAVGTVKSRIFRGKLELKKRLEHLFT"
     gene            complement(978..>1634)
                     /locus_tag="PKZ53_21890"
     CDS             complement(978..>1634)
                     /locus_tag="PKZ53_21890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ43151.1"
                     /translation="PGLKDQVAAPAAPAAPPAAALVESFAPAPVAERQFQFAPIESKP
                     SEETNTEVMLALLGIASRRQFDKFLDREWRRAARNHVPIALVIVDIDYFRSFREGFGN
                     QKGDDHLKAIAQTLNDELKRPGDLLGCYDHDQLIVLLPETPPDGALIVAEKLRAKIEA
                     LGLPNQRSPKGITTISLGLAALTPQQRATEASLIDAAELALYQAQRGGGNRVVSGHPL
                     "
BASE COUNT          358 a          417 c          463 g          396 t
ORIGIN      
        1 ggttccggga cctcccattc aggggccgca aacagtgagt tcgcctgctg aaattcagcc
       61 aggagttcac gggcttcggc tgaatccttt aaaaacgatt cgatttcgcg ccgacgcacg
      121 tctgaaagcg cgccttcaac ataggcttcc aggtcttcgc aaaaggcttc gaattgttgt
      181 tcagagtgtt cagcagacat aactttaggg cttagggctt ggggcttggg gcttggggaa
      241 atccaaatcc ggcctcaggt aaaaaggtgt tcgagtcgtt ttttgagttc cagtttgccg
      301 cgaaagattc gcgattttac cgtgccaacc gcaacattta aaatctcagc aatttcctca
      361 tatttcagtt cttcatactc tttgagcaag atggcgtccc ggaaatccgg atcaatttcc
      421 ttgagtgcct cgcgaaccag acgtgcccgc tcccgagttt cataatcctg agcgggcgag
      481 tcggtactga cggcttcttt gggtggcggc ccatcttctg agtcgtgatc ttgttcggcc
      541 accatgacct tgtgttcgct gcgacgaagc tcactgacgg cacggttaaa ggcgatgcga
      601 taaaaatacg tcttcaccga ggcttcttca cgccactcgc gcgcatgctg caccaccgcc
      661 ataaagactt cctgtgtcag gtcttcagcg cgggttgggc tgctgatttt gcgtgccaga
      721 aagcgataaa tcagaactcg atagcggttg tacagcacgg caaacgcagc ttcgtcgcct
      781 cgctgaaaat cacgcatgag ttcttcgtcg gttttgctca tgaattgggg tgaatgacgc
      841 gcatgcgttt aacctgtttc atcagcaggt atgtagtcgc aaggcgaaag aaaaggttcc
      901 acacaaaatg gcaggatgat acgtaaccag gtgacaactg accagttgtc acccagtcat
      961 tttgtcaggt tggtcagtta cagagggtgc ccgctgacaa cccggtttcc accgccccgt
     1021 tgtgcctggt acaaagccag ctcggcggca tcaattaatg atgcttcggt cgcgcgttgt
     1081 tgaggggtca gtgcagccag acctaaacta atggtggtga taccttttgg cgaacgttgg
     1141 ttggggaggc cgagggcttc gattttggcg cgaagttttt cggccacaat caaggctcca
     1201 tccggcgggg tttcgggcag taagacaatc aactgatcgt ggtcataaca ccccaggaga
     1261 tcaccgggcc gtttcagttc gtcgttaaga gtttgggcaa tggctttgag atggtcgtca
     1321 cctttttgat tgccgaaccc ttcgcggaac gagcggaaat agtcaatatc cacaatgacc
     1381 agcgcaatgg ggacgtggtt tcgggcggcg cggcgccatt cacgatccag aaacttgtca
     1441 aactgacgcc ggctggcaat gccgagcaag gccagcatga cttcggtatt ggtttcttca
     1501 gatggcttgg attcaatggg ggcaaactgg aattgccgct ctgcaacagg ggccggcgca
     1561 aagctttcaa ccaaagcggc ggcgggcggt gcggcgggtg cggcaggtgc cgcgacctgg
     1621 tctttcaatc cagg
//
LOCUS       DAOQDO010001697         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82943_length_1630_cov_3.925079, whole genome shotgun sequence.
ACCESSION   DAOQDO010001697 DAOQDO010000000
VERSION     DAOQDO010001697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82943_length_1630_cov_3.925079"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1630
                     /locus_tag="PKZ53_21895"
     CDS             <1..>1630
                     /locus_tag="PKZ53_21895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43152.1"
                     /translation="LATLPSNIIFQIQQDADGRIYATTNSGVVRLTFPANQPLSPETV
                     KVTTFTTEDGLPSGICNFGASLVDSQGRIWVGTAKGATFLETRADSENLQPRPLLIEN
                     VSNATHQIPLTPGTVLAYRENHLTFEFACLNYHRESEIRYQTQLIGFEESPTAWNQDS
                     KKEYTNLGAGTYIFRVWAKNHVGVVSGPAEFQFSITAAPWRTWWAYLMYGLVVGLIGL
                     GVIRLSQYRLERRNQLLETKIAERTVELAAKVKEVEASEQKALYASNAKSTFLANMSH
                     ELRTPLNAILGFAQLMAREHHRTSQDYEYLGIIIRSGEHLLSLINDVLSLSKIEAGKL
                     SLSEQVFDLKKMLRSLQEMFQLRAEAKRLRLEFRVAEDLPRLVTSDESKLKQVLINLI
                     NNAIKFTDQGSVILSVAPTEERIRFEVTDTGVGIGSHEFQHLFEPFVQTHSGLKTKEG
                     TGLGLAISREIIRLMGGEIKVESEIDRGSTFSFEIVLPASTSPESPVSTLSVSRVSAG
                     QPVYRMLVVDDHLENRLTLRILLESVGFEVQEAAN"
BASE COUNT          413 a          400 c          444 g          373 t
ORIGIN      
        1 ctggcgacat tgccgagcaa catcattttc cagattcaac aggatgccga tggacgcatc
       61 tatgccacga caaacagcgg agtggttcgg ctgacgtttc ctgccaatca gccactatca
      121 cctgaaaccg tcaaagtgac gacctttacc actgaggatg gattgccgag cggaatttgt
      181 aattttgggg catcgctggt ggacagtcag ggccgaatct gggtggggac ggccaaagga
      241 gcgacctttc ttgagaccag ggcggatagt gaaaacctgc aaccacggcc actgctcatc
      301 gaaaatgttt ccaacgccac tcatcagata ccactgacac caggtacggt cctcgcgtat
      361 cgggaaaacc atttgacctt tgaatttgcc tgtttgaatt atcaccgaga gtctgaaatc
      421 aggtatcaaa cccaactgat tggttttgag gaatcaccaa ctgcctggaa tcaggatagt
      481 aaaaaggaat acaccaatct tggtgctggc acctatatct tccgggtctg ggcaaaaaac
      541 catgtgggag tagtttccgg cccggctgag ttccagtttt caattacggc ggccccgtgg
      601 cgtacctggt gggcgtatct gatgtatggt ctggttgtgg ggctgatcgg cctgggggtc
      661 atccggctga gccagtatcg gcttgaacgt cgcaaccagt tgcttgaaac caaaattgcc
      721 gagcggacag ttgaactggc ggcaaaagtg aaagaagttg aggcgtcaga acagaaagcg
      781 ttgtatgcca gcaatgccaa gagcaccttt ctggcaaata tgagccatga attgcgcaca
      841 ccattaaacg ccattctcgg atttgcccag ttgatggcac gcgagcacca tcggacgtct
      901 caagactatg aatacctcgg aatcatcatt cgcagcggtg aacatctatt gagcctgatc
      961 aatgacgtgc tgtcgctttc caaaattgaa gctggaaaat tatcgttgtc agaacaggtt
     1021 tttgacttga aaaagatgtt gcgcagcctc caggaaatgt tccagttacg ggctgaggcc
     1081 aaacgcctcc gactggagtt tcgggtggcc gaagacctgc cccgcctggt caccagcgat
     1141 gaaagcaagc tcaaacaggt tttaatcaat ttaatcaaca atgcgatcaa atttaccgac
     1201 cagggcagcg tgatcctgtc agtggcgccg accgaggaac gaatccggtt tgaagtgacc
     1261 gacaccggag ttgggattgg ctcgcacgag tttcaacacc tgtttgagcc atttgttcaa
     1321 acgcatagcg ggctcaaaac caaggaaggc actggcctgg ggctagctat cagtcgtgag
     1381 atcattcggc tgatgggtgg tgaaatcaag gttgaaagcg aaatcgacag gggctcgacg
     1441 ttctcgtttg aaattgtgct tccagcctca accagcccgg aatctccggt ctcaaccctg
     1501 agtgtgagcc gggtgtcagc cggccagccg gtctaccgca tgctggtggt tgatgatcat
     1561 ttagaaaacc ggctgacctt gcgtatcctg ctcgaatcgg ttgggtttga agtccaggaa
     1621 gcggcaaatg
//
LOCUS       DAOQDO010001698         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82967_length_1630_cov_2.808254, whole genome shotgun sequence.
ACCESSION   DAOQDO010001698 DAOQDO010000000
VERSION     DAOQDO010001698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82967_length_1630_cov_2.808254"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKZ53_21900"
     CDS             <1..670
                     /locus_tag="PKZ53_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43153.1"
                     /translation="GGSAIQTNSFEPVAAWIGICTGVAGLALISSVCERETLTRRIRR
                     TIPTNPISRIVAFFFYSGSANGLIWSLAFMGITLLIGATLAETITRFKDRGDLDKAIL
                     MGFGTTMYFFNYSMTALFIRRLLPGEKVKPFLTWIFVLVLMALGSAVPPILAFLVSFE
                     SADTAWLLLNPYAFVIDSAGKSQWLFFFVVGIWGLAVLGIHLKWLLTQIRNFTPLTPA
                     PVSE"
     gene            711..1583
                     /locus_tag="PKZ53_21905"
     CDS             711..1583
                     /locus_tag="PKZ53_21905"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNJ43154.1"
                     /translation="MEPRFRQYLQEGSQAGVRYALSVPQRVTLGTAGMHVGSRAGSSL
                     EFKDHREYQSGDDLRRIDWNAYARSDKLIVKLYREEINPHLDIVVDGSRSMNLEDTPK
                     CQVTLGLAALFAEASANAGYTHAAWIVRNGCEKVANGANRPHVWDGIAFDATEPLPAL
                     LNRTPPNWYRQGVRVLISDLLWMDNPLHTLQHLARNASTIYVIQVLARADINPEDQGN
                     LCFVDSETGELREIYVDATVTKRYRERLAQHQQNWHVACKQVGARLATVIAEDVVSNW
                     HLDGLLSEEILKLA"
BASE COUNT          352 a          441 c          431 g          406 t
ORIGIN      
        1 gggtgggtca gctatccaaa ccaattcgtt tgaacctgtt gccgcctgga ttggcatctg
       61 cactggggtc gctggcctgg cactgatttc atcggtctgt gagcgcgaaa cgctgacccg
      121 ccggattcgt cgcaccattc cgaccaatcc aatcagccgc attgtggcct ttttctttta
      181 tagcgggtca gccaatggcc tgatctggtc actggctttt atgggtatca cgctcctcat
      241 tggcgccaca ctggccgaga ccatcaccag gtttaaggat cggggtgacc tcgacaaggc
      301 cattctgatg gggtttggca ccacgatgta ctttttcaat tattcaatga cagccctgtt
      361 catccgccgc cttttgccag gagagaaagt gaaaccattt ttaacctgga ttttcgtgct
      421 ggtcttgatg gctttaggta gtgccgttcc gccgattctg gcgtttttag tcagcttcga
      481 atctgccgac acggcctggt tgctcttgaa cccctatgcg tttgtgattg atagtgccgg
      541 caaatcacag tggcttttct tctttgtcgt gggtatctgg gggctggccg tgctgggtat
      601 tcatctcaaa tggctgctga cgcaaattcg taactttacc cctctcacac ccgccccagt
      661 ttctgaatga caaggtgacc ggttctgact actgactact tcactcacat atggaaccac
      721 gctttcgcca atatttgcag gaaggatcac aagccggggt ccgatatgcg ctgtccgtgc
      781 cgcaacgggt cacgctaggc acagccggaa tgcacgttgg gagccgtgcc ggcagctcgc
      841 ttgagttcaa agaccatcgc gaataccagt ctggcgatga cctgcgacgc attgattgga
      901 acgcctatgc ccggagcgac aaactgatcg ttaaactcta tcgcgaagaa atcaatccac
      961 accttgacat tgtggttgac ggttcacggt caatgaatct tgaagacacc cccaaatgtc
     1021 aggtcacact tgggttagcg gccttgtttg ccgaagcttc ggccaatgcc gggtataccc
     1081 acgccgcctg gatcgttcga aacggatgcg aaaaagtggc caatggcgcc aatcgtcccc
     1141 atgtctggga tggaattgcc tttgatgcga ctgaaccgct tccggccctg ctcaatcgca
     1201 cacctccaaa ctggtaccgg caaggagtcc gagtcctgat aagcgatctg ttgtggatgg
     1261 ataacccgct ccatacgctg caacatctgg ctcgcaatgc ctccaccatc tatgtgatac
     1321 aggttttagc ccgcgccgac ataaatccgg aagatcaggg aaacctgtgt tttgtggatt
     1381 ccgaaactgg cgaactccgt gaaatttatg tggatgccac cgtcaccaaa cgctatcgcg
     1441 aacgactggc ccaacatcag caaaactggc acgtggcctg taaacaggtc ggagcccggc
     1501 tggccacagt cattgccgag gacgtggttt caaactggca tctggatgga ctgctatcgg
     1561 aggaaatttt gaaactggca tgagacctgg gttcagggtt cagggttcag ggttcagggt
     1621 tcagggttca
//
LOCUS       DAOQDO010001699         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_82968_length_1630_cov_2.762540, whole genome shotgun sequence.
ACCESSION   DAOQDO010001699 DAOQDO010000000
VERSION     DAOQDO010001699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82968_length_1630_cov_2.762540"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..>1630
                     /locus_tag="PKZ53_21910"
     CDS             314..>1630
                     /locus_tag="PKZ53_21910"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ43155.1"
                     /translation="MSLKTRIFLTFSAVSLATCLSGPAKAQTQPNSTPTSSAPALTAS
                     SSASAQSLIDKAKQRFKQGETLFREGKTEEARALFDQAVDMLLSAPADVRNHADVQTY
                     YFDLINKVHLLEVGALQSDDDTQETATPSPLDELATIDINKENVEDKTKQIDTDKFGF
                     KFTVTSEVYKFISFYTNGRGRSTMEHGLARSGRYRAMAERIFAEEGVPTDLIWLAQVE
                     SVWQPSALSWAKAKGIWQFIPGTGQRFGLQQNGWVDERVAPEKATRAAARYLKFLNNY
                     FAGDWLLAMAAYNSGEGNVERAIARCGYADFWELHSRNLLPNETRNYVPAILAVIAVA
                     KDQKNYGFNVRPEPAWKFDPVTIDDQIDLTVAANLLNVSEESLKALNPELKRGVTPPG
                     QYTLKVPAGTKQQFEVAYAALPADQRMRRTPMFDGPDTDTDGGLELA"
BASE COUNT          411 a          478 c          384 g          357 t
ORIGIN      
        1 acccggaacc cggaacccgg aacccggaac ccggaacccg gaacccggaa ccccaactcc
       61 ctgtctacag agtagccaat gcgcgggcgc gctaaaccac acggtaaaag ctttcaaatt
      121 ttaaataaaa ataagagtgg aagggttgac ctgtctttcc aagcgatgct ataacgcttt
      181 cgcacactta gtccggtaaa tttcccttat ccactctcgc ctctttattt catatcagac
      241 cgggaaatcc ctgagcagtt tcaaccaggc tgcattcata tctttttgtt ctacataagg
      301 agacaaggct gcgatgtctt taaaaactcg tattttcctc accttctccg ctgtttcgct
      361 ggcaacctgc ctgtcggggc cggcgaaagc tcaaacacaa ccgaattcga ccccaacgag
      421 ttccgcacct gcccttaccg cttcctcctc tgcctctgcc caatcactca tcgacaaagc
      481 caaacaacga tttaaacaag gtgaaacttt gtttcgtgaa ggcaagaccg aagaagcccg
      541 tgccctgttt gatcaggccg ttgatatgtt gctgagcgca ccggctgatg tccgcaacca
      601 cgctgacgtg caaacctact attttgactt gatcaacaaa gttcacctgc tcgaagttgg
      661 ggcattgcag tcagatgatg acacccagga aacggctacc ccgtcgccgc tcgacgaact
      721 ggcgaccatt gatatcaaca aagagaacgt cgaagacaaa accaaacaga ttgacaccga
      781 caaatttgga ttcaaattta cggtgacctc cgaagtctat aaattcatca gcttctatac
      841 caacggtcgc ggtcgcagta cgatggaaca cggtctggct cgctctggcc ggtatcgtgc
      901 gatggctgaa cggatttttg ccgaagaagg cgtccccacc gatttgatct ggctcgccca
      961 ggttgaatca gtctggcaac cctcagctct ctcctgggcc aaggccaaag gcatctggca
     1021 gtttatccca gggacgggac aacgctttgg gctccagcag aatggctggg ttgacgaacg
     1081 ggttgctcca gaaaaagcca cgcgcgccgc cgctcggtat ctcaagttcc tgaacaacta
     1141 ttttgctggt gactggctgc ttgcgatggc agcctacaac agcggcgaag gcaacgttga
     1201 acgggcgatt gcccgctgcg gatacgccga tttctgggaa ctccacagcc ggaatctgct
     1261 tccgaacgaa acccgcaatt atgtgccagc tattctggcc gtgattgctg ttgccaaaga
     1321 tcagaaaaat tacggcttta acgttcgccc cgagcctgcc tggaaatttg atcccgtcac
     1381 gattgacgat caaattgacc ttaccgtagc ggccaacctg ctcaacgttt ctgaagaatc
     1441 cctcaaagcc ctcaatccag aactcaagcg tggcgtcaca cctccaggac agtacacgtt
     1501 aaaagttcca gccggcacca agcaacagtt tgaagtggcc tatgcagccc tgccagccga
     1561 tcagcggatg cgccgcacgc cgatgtttga cggtccagac actgataccg atggcggcct
     1621 tgaactggcc
//
LOCUS       DAOQDO010001700         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83065_length_1629_cov_3.421220, whole genome shotgun sequence.
ACCESSION   DAOQDO010001700 DAOQDO010000000
VERSION     DAOQDO010001700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83065_length_1629_cov_3.421220"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PKZ53_21915"
     CDS             complement(<1..750)
                     /locus_tag="PKZ53_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43156.1"
                     /translation="MEDLLQQATLDFESFLSGHKAPALVGHSLMTIMKNDVRTIAHIT
                     MTWAVKQTSGALPQNLMQARNKVFDIFFYRVIKFEGIYKFFPLFEEALLQFCPPNFRG
                     QLTALFTQYSWQDIRPIGDIKQQPVFALEKRTAAEVKEEQFNEDVYKNVTHNILALES
                     RFTFADEQQGAEMAQHTARISTLFKDFVGLVKDKDARREIIIANEGDKDAMYENKKKF
                     NLDDYIAQCLDLTVAFFNDDYLLQSVQMMDLL"
     gene            complement(1241..1432)
                     /locus_tag="PKZ53_21920"
     CDS             complement(1241..1432)
                     /locus_tag="PKZ53_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43157.1"
                     /translation="MKNHTGRYFIFMLLFVIFPLQRLVASPHFETMRAVDMLLLFASG
                     MMAGVLIIQIKQHRNHPGQ"
BASE COUNT          420 a          362 c          414 g          433 t
ORIGIN      
        1 cagcaaatcc atcatctgga cgctttgcag caggtagtcg tcattaaaaa aggcgacggt
       61 caggtccaga cactgggcga tgtagtcatc aagattaaat ttcttcttgt tttcatacat
      121 cgcgtcttta tcgccttcat tggcaataat gatttcgcga cgcgcatcct tgtctttgac
      181 caatccaacg aaatctttga acaaggtgct gatacgggcc gtgtgctggg ccatttcagc
      241 gccttgttgt tcgtcagcaa acgtaaaccg gctttcaagg gccagaatgt tatgcgtgac
      301 gtttttatag acgtcttcgt tgaactgttc ttctttgact tcagccgccg tccgtttttc
      361 aagcgcaaag accggttgct gcttgatgtc gccaattggg cgaatgtcct gccagctata
      421 ttgggtaaac aaagccgtga gttggcctcg aaaattcgga gggcaaaatt gaagcaacgc
      481 ttcttcgaat aatgggaaaa atttatagat tccttcaaat ttaataacgc gatagaaaaa
      541 gatatcaaaa accttgtttc gggcctgcat caggttttgg gggagtgctc ccgacgtttg
      601 tttaacggcc caggtcatgg tgatatgcgc aatcgtacgc acatcatttt tcatgatggt
      661 catcaacgaa tgcccaacca gtgctggggc tttatggcct gacaaaaagg actcgaaatc
      721 aagggttgcc tgctgaagaa ggtcctccat agcaacgaac tcctctgctg gtgtctttcg
      781 gcaaacagtc tgtttttggg tatggttcag gaatccgcca gagaaatgaa ccggcattgc
      841 tggtaatact ctgaaagaga ttatcttcca atgagttgca aagagcctgt accaggtgat
      901 tatgctggcc aactgaccga atgccgaacc tgccgtgtgc ggcttgagtt ttcggtttcc
      961 ctggatctgg aagttgtttc ctgtgtattt tggctaagat atgcggggat gcgcaactat
     1021 atgtgttttg cgcaaaaaag gaaaccttca gtgtgggata tgggaaagaa gtgaagattg
     1081 cagccagaga gcgcaggcgt gagggggtca aagctgatca aaaaagccat tggacctggg
     1141 gaatcaccgg ttggttcatt ctccgaaaac caggttccgt gggtggcgtc aacgcagacc
     1201 cacggtgagt acacattgat tgcagatgag cggcacaaca ttactggccg gggtggttgc
     1261 ggtgctgttt gatctgaatg atcaacaccc cagccatcat tcccgaggca aagagcagga
     1321 gcatatccac tgcccgcatt gtttcaaagt gtggtgacgc caccagccgt tgtagcggga
     1381 agatcacaaa gagcaacatg aaaataaaat atcgacctgt atggtttttc ataacaaatt
     1441 caatcctttc agtgagttaa aatggttagg cattcgcttg atatctggag tcgagtgata
     1501 cgtattcttc aattctcaat tcttcattct tcatccctga ttgggcagtt tgccagttac
     1561 cacacgaaga agaatggtga ccaggacgcc gccgacgcat tcgagatgct gctgcgcaaa
     1621 cactcggcg
//
LOCUS       DAOQDO010001701         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83067_length_1629_cov_3.337357, whole genome shotgun sequence.
ACCESSION   DAOQDO010001701 DAOQDO010000000
VERSION     DAOQDO010001701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83067_length_1629_cov_3.337357"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..517)
                     /locus_tag="PKZ53_21925"
     CDS             complement(125..517)
                     /locus_tag="PKZ53_21925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ43158.1"
                     /translation="MPHFFQVTPRVPVTDLRRTIDFYTQTLGFTEDVVWPEDQPTFCI
                     LTRDTVSLGFFVPDGCRSDTKPGTCDLYIEVDGAQELHAELKDKLTIEWGPEVYAYGR
                     REFAFRDPDGYLIIVTEETDDPPTCQVD"
     gene            complement(647..1084)
                     /locus_tag="PKZ53_21930"
     CDS             complement(647..1084)
                     /locus_tag="PKZ53_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ43159.1"
                     /translation="MTAPFRKSFLAGWGDMDFNAHMRNTSYLDRSGDVRMMYFQEHGF
                     PMSEFARLRIGPVILRDEIEYFRELHLLDPFEVTLTLSALTSDASRFRLRNEFFRQDG
                     KMCARVTSHGGWLDLATRKLTIPPAELIEAIRAIVRSEDFAEL"
     gene            complement(1160..>1629)
                     /locus_tag="PKZ53_21935"
     CDS             complement(1160..>1629)
                     /locus_tag="PKZ53_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43160.1"
                     /translation="LAVIILAGMCGWSTVPALFQPNSAKTQKTKKKKAVPSDEYSDPE
                     TDRAFQLEEDADLVVGVGRHRIWVSCPGKPTETALSEEYLVAALPFLDLKKKELVVVV
                     FDKTMIGDYYGFRLKDVMDRLEKAFLENGFKKIVFQQGTATGRPILREIERRP"
BASE COUNT          402 a          405 c          396 g          426 t
ORIGIN      
        1 gatgagggct tggggctgaa gatgttgggt tcaaggcaaa atctccaatt gtctccagcc
       61 cgctttcttc agcccgtctt cttcagcccg tcttcttcag cccgtcttct tcagcccagg
      121 tttttcagtc cacctgacag gttggcgggt cgtcggtttc ttcggtcacg ataatcaggt
      181 agccgtctgg atcccggaag gcaaattccc ggcggccata agcataaact tccggccccc
      241 attcaatcgt gagtttgtct ttgagttcgg cgtggagttc ttgtgcgccg tcaacttcga
      301 tatacaggtc acaggtcccc ggtttggtat ctgatcggca gccatctggt acaaaaaagc
      361 cgaggctgac ggtatcacga gtcaggatgc agaaagttgg ttgatcttcg ggccagacaa
      421 catcttcagt aaaaccaaga gtttgagtgt aaaagtcaat tgtgcgtcga aggtcagtga
      481 cgggaacacg cggagtaacc tggaaaaagt gtggcatagg atgtcctcaa cgatgaaagt
      541 atgcggattt gcatcacgga ttgcccttct cctataacac agtaattcat tattttccag
      601 aaaaacactt cgagttgggg ttcagtgtcc tgaagtgaca aaaaaattag agttctgcaa
      661 agtcttcact gcggacaatc gcccgaattg cttcaatgag ttcggctggc ggaatggtca
      721 atttccgggt cgccaggtcc agccatccgc cgtggctggt aacccgagca cacatttttc
      781 catcttgacg aaaaaattca ttccggagcc gaaatcgtga agcatcagac gtcagtgccg
      841 agagcgtcag ggtaacttca aatggatcaa ggagatgcaa ctcacggaag tattcaattt
      901 catcgcgcag aatcaccggg ccaatccgca agcgggcaaa ttcgctcatt ggaaaaccgt
      961 gttcttgaaa gtacatcatt cggacgtcgc ccgaacggtc aaggtacgag gtattgcgca
     1021 tatgcgcgtt aaaatccata tctccccagc cagcgagaaa acttttgcgg aacggagccg
     1081 tcatgcagat ggtgattcct ttcgagtgga tttgcaggtt gtgaaacttt ttgcatcttg
     1141 ccagaaagaa agggaatttt catggcctcc gctctatttc ccgtaagatt ggccttcccg
     1201 tagccgttcc ctgttgaaag acaatttttt tgaagccatt ctccagaaaa gctttctcta
     1261 atcggtccat cacatctttc agccgaaacc catagtaatc tccgatcatc gtcttatcga
     1321 aaacaacgac caccagctcc ttctttttca agtcgagaaa tggcaaggcg gcaacgaggt
     1381 actcttcact gagcgcggtt tcagtcggct ttcctgggca gcttacccaa atccggtgtc
     1441 ttcctacacc tacaaccaaa tcagcatctt cttcaagttg gaaagcacgg tcggtttcag
     1501 gatcagaata ttcgtccgag ggaaccgcct ttttcttttt ggtcttttgt gtttttgcgg
     1561 agtttggttg gaaaagcgct ggaaccgttg accagccgca cattccagcc aggattatta
     1621 cggcaagaa
//
LOCUS       DAOQDO010001702         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83082_length_1629_cov_2.987294, whole genome shotgun sequence.
ACCESSION   DAOQDO010001702 DAOQDO010000000
VERSION     DAOQDO010001702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83082_length_1629_cov_2.987294"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1629)
                     /locus_tag="PKZ53_21940"
     CDS             complement(<1..>1629)
                     /locus_tag="PKZ53_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43161.1"
                     /translation="VITAQTRLIQSWIGLGIGVALTGSFCTAVIGVQNRLYFSNDLRT
                     ALWMLAGFGLAGCAIRFHQKSQLEEDKERHPLSPVHYVTELTWVAQLVVLGSAVLVGY
                     AMLSSPRMAAIGLGAELAALALVTSRMVKTEVELGWLCLTAWMSLLGSWWHTVSFLKF
                     PDGWSYLPQTIIWATAGYVLLWAALDRFRKATEQEILAANGLPEYQAKAARQRIIGQT
                     LWAVSYVVLAAGLEMLAYTWPHLSHPFYRIWPSVLLTIELAGLLLICGHWIRQTHLGI
                     VHLIGGMGLMAFGYAAFIDYLPLKNPRWEMPLIALWLLVQHGFARIAVSQSWTQDERW
                     AKAARLTNDLCAFPVLVVNFFILPMAVVESFPAKEAELLTFTLITVIWASWVLSSQTT
                     WYHKIGLGLSLSSTWAVVLNYLGIKPAYFPAAYLFLGFLLLVLSLQLLPRLNRNREAS
                     HFLANPLEHIGHFISLVSLAGLVGQALVWLPDLRRDEQYLLVALMLGLLSTTLISIIT
                     RGTAWRKFYAPASYLLGGLFYVRIGLFLGYGLFR"
BASE COUNT          428 a          457 c          422 g          322 t
ORIGIN      
        1 tctctgaaca atccgtagcc caaaaacaac cctatccgaa catagaacag ccctcccagg
       61 aggtaactcg ccggagcata gaattttcgc caggctgttc cacgggtgat gatgctgatc
      121 aacgtcgtag agagcaagcc aagcattaac gccaccagca ggtattgctc atcccgacga
      181 agatccggca accagaccag cgcctgtccc accagccctg ccagtgagac cagactgatg
      241 aaatgaccga tgtgctcaag cgggttggcc aggaagtgag atgcttcgcg gttgcggttc
      301 aggcgtggca ggagttgaag gctcagtacc agcaacagga acccaaggaa cagataggcg
      361 gctgggaaat aggctggttt gattcccaga taattgagta ccacggccca ggttgacgaa
      421 agcgataacc ccaacccgat cttgtggtac caggtcgttt gtgaactcaa cacccagctt
      481 gcccaaataa cggtaatcag cgtgaaggtg agtaattcag cttctttggc cgggaatgat
      541 tccaccacgg ccatcgggag aatgaaaaag ttgacgacca gtaccgggaa cgcacacagg
      601 tcattggtca gccgggcagc ttttgcccac cgctcgtctt gcgtccagct ttgggaaacg
      661 gcgatccgcg caaatccatg ctggacaagc agccagaggg caatcaatgg catttcccat
      721 cgcggatttt tcagcggcag gtaatcaatg aatgccgcat agccgaatgc catcagcccc
      781 attccgccga tcaaatgcac tattcccagg tgtgtctggc gaatccagtg tccacaaatc
      841 aacagcaggc cagcaagttc gattgtcaga aggacgctcg gccagatccg gtaaaagggg
      901 tgtgaaaggt gtggccaggt gtacgccagc atttccagtc ctgctgccag gaccacataa
      961 ctgactgccc agagtgtttg accaatgatt cgctgacgag ccgctttagc ctgatattcg
     1021 ggcaggccat tggcggccag aatttcctgc tctgtggctt ttcggaatcg gtcaagcgcg
     1081 gcccagagca gcacatatcc tgccgttgcc cagataatgg tctgtggcag atagctccat
     1141 ccatcaggaa atttgagaaa ggaaaccgta tgccaccacg acccaagcaa cgacatccag
     1201 gccgtcaaac acaaccaccc taattcaact tcagttttca ccatccgtga agtcaccagc
     1261 gccagggcag ccagttcagc acccaggcca attgccgcca tacgtggtga agacaacatc
     1321 gcatacccga ccagaaccgc tgacccgagc accacgagtt gggcgaccca ggtcagttcc
     1381 gttacataat ggacgggtga aagtgggtga cgttctttgt cttcttcgag ttgtgatttt
     1441 tgatgaaagc gaatggcaca tccggcgagg ccaaacccgg caagcatcca cagggcagtt
     1501 cgcaggtcat ttgaaaagta cagtcggttc tgaacaccaa tcaccgcagt acaaaaactt
     1561 ccggtcaggg ccacgccaat tccaagacca atccagcttt gaatcaatcg ggtttgagcc
     1621 gttatgacc
//
LOCUS       DAOQDO010001703         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83088_length_1629_cov_2.859593, whole genome shotgun sequence.
ACCESSION   DAOQDO010001703 DAOQDO010000000
VERSION     DAOQDO010001703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83088_length_1629_cov_2.859593"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /gene="ftsA"
                     /locus_tag="PKZ53_21945"
     CDS             <1..1123
                     /gene="ftsA"
                     /locus_tag="PKZ53_21945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNJ43162.1"
                     /translation="VRKGVIVNIESAEECIRQALTEAEHMAGVDAHQVHVGLSGAHVR
                     GHNGRGVVAISRKNREITVADIRRVIDQASAINLPSDREIVEVLPQEFIVDDQDGIGD
                     PLGMLGMRLEVIVHIVTSPITAKQNIVTSVNRSGMIVSDLTLESLAAAEATLSEEERE
                     YGVALIDIGGEITSLAIYQRGAVRHTSVIPLGGTHFTNDIAVGLRAPFPEAERIKRHF
                     GCSFGPLLDTCERETVLEVLNVGGRVSKTLSRQLLCDMLQPRAEELFCQIQEEIHHSG
                     YERQLSSGLVLTGGGSLLAGVPEIAERILDVPVRLGSPQGFDGIADDLISPEYATVAG
                     LVLHAARQQVLGRTSWNQNGLRRHSARLKNWWLELKNLF"
     gene            1276..>1629
                     /locus_tag="PKZ53_21950"
     CDS             1276..>1629
                     /locus_tag="PKZ53_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNJ43163.1"
                     /translation="MAADNNRTDNSLKLEFVDIPPTNGANIKVVGVGGGGSNAVNRMI
                     EFGIEGIKFLVVNTDLQALRMSHAPVKLQVGAKLTKGLGAGANPEIGRQAALEDTDKI
                     IEALEGADMVFITSGM"
BASE COUNT          383 a          424 c          458 g          364 t
ORIGIN      
        1 ggttcgcaag ggcgtaattg tcaacattga atcagcggag gagtgtatcc gtcaggcgct
       61 caccgaagct gaacatatgg ccggcgtgga tgcccatcag gtccatgtcg ggctttccgg
      121 tgcccacgtc cgcggccaca atgggcgtgg cgtggtggcc atttcacgca aaaaccggga
      181 aattaccgtc gctgatattc ggcgcgtcat tgatcaggcc agtgcgatta acctcccgtc
      241 ggaccgggag attgtcgaag tactgcccca ggagtttatt gttgatgatc aagacggaat
      301 tggcgatccc ctcggtatgt tggggatgcg cctggaagtc atcgtccata ttgtgacttc
      361 ccccatcaca gcgaagcaaa atatagtgac aagcgtaaac cgttcgggta tgatcgtttc
      421 ggatctcaca cttgaatccc tagcagcagc cgaggctaca ctgagcgaag aagagcgcga
      481 gtacggtgtc gctctcatcg acatcggtgg cgaaatcacc agcctggcga tctaccagcg
      541 tggagctgtt cgccatacct cagtcatccc gcttggcggt actcatttca ccaatgacat
      601 tgcagttggc ctccgtgcgc cgttcccgga agcagagcgg atcaagcgcc actttgggtg
      661 ctcttttggc ccattgcttg atacctgtga acgggaaact gtccttgaag tgctcaatgt
      721 cggtggacgg gtttccaaga cgctgtcccg gcagttgctc tgcgatatgc ttcagcctcg
      781 cgcagaggag ctcttttgcc agatccagga agaaatccat cacagcggat atgaacgcca
      841 actctcaagc gggctcgtcc tgactggggg tgggagtttg ttggctggtg tcccggaaat
      901 agccgaacga atcctggatg ttcccgtccg actcggatca ccgcaaggct tcgacgggat
      961 tgcggatgat ttaatcagcc ctgaatatgc cacggtggcg gggctggtcc tgcatgcggc
     1021 tcgtcaacag gtactggggc ggacctcgtg gaatcaaaat ggccttcgcc gccacagtgc
     1081 ccggttaaaa aactggtggc ttgaactcaa aaacctgttt taaccccccc tgtttcgccc
     1141 ctctatcaga ggccaacaga catcagaagt aacctttttc aaaacaatca tataacgcgg
     1201 ggcacatccc ccatccaggc atatatttta agaacaagca ggggaacaag gctcctgtga
     1261 ggaggcaata agtacatggc agcagacaac aatcgcacag ataattcatt gaaattggaa
     1321 ttcgttgata ttcctccaac aaatggggca aatattaaag tggttggggt tggcggtggt
     1381 ggaagcaatg ccgtcaaccg catgattgag tttgggatcg aaggaatcaa atttctggtc
     1441 gtgaacactg atctccaggc cctgcgcatg tcacatgccc cggtaaaact tcaggttggg
     1501 gccaagctta ccaaggggct cggggcgggc gccaaccctg aaattggtcg gcaggcggct
     1561 ctggaagata ccgataagat tattgaagcc ctcgaaggcg ccgacatggt gtttatcacc
     1621 agcggcatg
//
LOCUS       DAOQDO010001704         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83286_length_1627_cov_2.317430, whole genome shotgun sequence.
ACCESSION   DAOQDO010001704 DAOQDO010000000
VERSION     DAOQDO010001704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83286_length_1627_cov_2.317430"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1627)
                     /locus_tag="PKZ53_21955"
     CDS             complement(<1..>1627)
                     /locus_tag="PKZ53_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNJ43164.1"
                     /translation="ATSVTRERWLLPLFQELGYGRLMSVKAFTIEDKPYAISHLWQDT
                     PIHLVGVGIEIDRRTAGATGAARQSPHSLVQEFLNRSEAHLWAFVSNGLRLRVLRDNK
                     SLTRQAFIEFDLEAMFEGQLYSDFVVLWLVCHQSRVEAERPEHCWLEKWSQTARTQGT
                     RVLDQLRDGIQQAISALGSGFLAHPANAALREKLRSGTLSPQDYYRQLLRTVYRLLFL
                     CVAEDRDLLHDPNGDLTAKERYFKYYSVRRLRQMAGSIRGTQHTDLWHGISTVFGKLD
                     SDGCEQLALPALNSFLWASSSIPDLPPTLQITNRAVLDALRVMVFTVEKNTRRPVDFK
                     NLGAEELGSVYESLLELIPEINTDTCTFELKTASGNERKTTGSYYTPTSLIQCLLDSA
                     LDPVLDEAARNSEAEQAILDLKVCDPACGSGHFLIAAAHRIAKRLAMVRTGDEEPSPE
                     TLRHALRDVIGRCIYGVDINPMAVELCKVALWMESLEPGKPLSFLDHHIQCGNSLLGT
                     TPELLKNGIPDEAFTPIEGDDKAVCSELKKRNKK"
BASE COUNT          359 a          442 c          467 g          359 t
ORIGIN      
        1 cctttttgtt gcgttttttg agttcgctgc acacggcttt gtcatcgcct tcgattggtg
       61 tgaaggcttc gtcgggaatt ccattcttca aaagctcagg tgtcgtgcca agcaaactgt
      121 tgccgcactg aatgtggtga tcgagaaacg acaggggctt gccgggttcg agcgattcca
      181 tccagagcgc gactttgcac agttcaaccg ccatcgggtt gatgtctacg ccatagatgc
      241 accgcccaat cacatcccgg agcgcgtggc gaagcgtttc aggcgaaggt tcttcatcac
      301 cggtgcggac catggccagc cgtttggcaa tccggtgcgc cgccgcaatc agaaaatgac
      361 cgctgccgca ggccgggtca cacactttca agtccagaat cgcctgttcc gcctctgagt
      421 tgcgggcggc ctcgtccaaa accggatcca gcgccgaatc cagcaggcat tgaatcaaac
      481 tcgttggggt gtagtaactt ccggtggttt tgcgttcgtt gccgctggcg gttttgagtt
      541 caaaggtaca ggtatccgtg ttgatttcag gaatcaattc aaggagcgat tcatagacgc
      601 tgccaagttc ttcagcccca aggtttttga aatcaaccgg gcggcgggtg ttcttctcca
      661 cggtgaaaac catgactcgc aacgcatcca gcaccgcccg attggtgatt tgaagtgttg
      721 gaggaaggtc agggattgac gaactggccc agagaaaact gttgagtgcc ggcaacgcca
      781 gttgttcaca gccgtctgaa tcaagttttc caaagacggt tgaaatgccg tgccacaaat
      841 cggtgtgttg cgtgccgcga atcgagcccg ccatctgccg caaccgccga accgagtagt
      901 atttgaaata gcgttctttg gctgtcaggt caccgtttgg gtcgtggagc aaatcgcgat
      961 cttccgccac gcacaaaaac aacagccgat acacggtgcg cagcaattgc cggtagtaat
     1021 cctgcggaga aagcgtgccg ctccgcagct tttcccgaag tgcggcattg gcgggatggg
     1081 ccagaaaacc gcttcccagc gctgaaatcg cctgctggat tccatcccga agctgatcaa
     1141 gcacccgcgt gccctgggtt cgggccgttt gcgaccattt ttcaagccag caatgttcgg
     1201 ggcgttcggc ttcaacgcgt gactggtggc acaccagcca gagcacgaca aaatcggaat
     1261 aaagctgacc ctcaaacatg gcttcgaggt caaattcaat aaatgcctgg cgggtgagtg
     1321 atttgttgtc gcgcagcact cgcaaccgca ggccgttgga gacaaacgcc cagagatggg
     1381 cttccgaacg gttgagaaat tcctgaacca gactatgcgg ggactgccgg gcggcgccag
     1441 tggcaccagc cgttcggcgg tcaatttcaa ttccgacgcc gaccagatga atcggggtgt
     1501 cttgccagag atgagaaatc gcgtagggtt tgtcttcgat ggtaaaggct ttcaccgaca
     1561 tcagccgacc gtagccaagt tcttgaaaca gcggcaggag ccagcgttca cgggttacgg
     1621 aggtcgc
//
LOCUS       DAOQDO010001705         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83346_length_1626_cov_3.378740, whole genome shotgun sequence.
ACCESSION   DAOQDO010001705 DAOQDO010000000
VERSION     DAOQDO010001705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83346_length_1626_cov_3.378740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>1626)
                     /locus_tag="PKZ53_21960"
     CDS             complement(297..>1626)
                     /locus_tag="PKZ53_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43165.1"
                     /translation="LMKPWDPPDEHLYPVLTDLLDDWSSGYQPPFEGIRLIGHRWSPE
                     THRLKEFLARNQVPFRWLDVESNLDAQSLVLVSRLTNPTFPLVSFPDGTVFTAPTEAE
                     VAQKIGLRKEAEMPFYDLVIIGAGPAGLAAGVYGASEGLRTLLIERHAPGGQAGTSSR
                     IENYLGFPVGLSGGDLARRAVAQARRLGVEILAPEEVVAIQDVSPYRSVSLKDGRSIN
                     CYAVLIATGVSYCPLDVPGVHRLTGAGVYYGAAMTEAISCQGEAVFIVGGANSAGQAA
                     MYFSKYACTVTMLVRGNSLEQRMSQYLVDQIRSTPNIVVRLNTAVVGVDGEQNLESVT
                     LSDLKTGSREVLPARALFIFIGAVPQTDWLGTTVARDPRGFILAGPDITGNGTRPAGW
                     KRDPFLLETSLPGVFVAGDVCANSVKRVASAVGQGSIAIQFIHQYLSLVK"
BASE COUNT          427 a          433 c          409 g          357 t
ORIGIN      
        1 ggaatacacg gaatacacag aaaataaatc cagattcttc aatggtttct gaaatctcaa
       61 gacatagaaa cccaagaaat atttatctga gaagctatag ccatttgcag tggcgccccc
      121 ggattagaaa acagtcacat gacccaacca aacaaactga gcgaactcct gacttctgac
      181 tggcaagtcc cttgtcaaaa gtttcctgag acaatgaatt ttggaagtgg ttgatttttt
      241 tcgtgtattt cgtgtgtttc gtggttcaaa atgtctggaa cttttcagta atgaactcat
      301 ttcaccagac ttaaatattg atgaataaac tggatggcaa tcgaaccctg accgactgcc
      361 gacgctaccc gtttgaccga attggcacag acatcccccg ccacaaagac accagggaga
      421 ctggtttcca gcaaaaaagg atctcgtttc caaccagcag gccgggttcc attgccagtg
      481 atgtctggtc ccgccaggat aaacccacgt ggatctcggg ccacggtggt tccaagccag
      541 tcggtttggg gcacggcgcc aataaaaatg aagagcgccc gggccggaag tacttcccga
      601 cttccagttt tcaaatcgga aagtgtaact gattcaaggt tttgctcgcc gtcaacccca
      661 accacggcgg tattgagccg aaccacgata ttgggtgttg atctgatttg gtcaaccaga
      721 tattgagaca tgcgttgttc gagggaattg ccccgcacca gcatcgtcac agtgcaggca
      781 tactttgaaa aatacattgc cgcctggccg gcggaattgg cgccaccaac aataaagact
      841 gcttcaccct gacaggaaat ggcttcggtc attgctgcgc cataataaac acctgcacca
      901 gtcaggcgat gcacacccgg aacatcgagt ggacagtatg aaactccggt tgcaatcaaa
      961 accgcataac aattgatact acggccatct ttcaaactga ctgagcgata gggagacaca
     1021 tcctggatgg cgacaacttc ttccggggca agaatttcca ccccaagtcg cctggcctgg
     1081 gcaactgccc ggcgcgcgag gtcgccaccg ctgagcccaa ccggaaaacc aagatagttt
     1141 tcaattctgg aactggtacc ggcctgcccg cctggggcat ggcgttcgat cagcagcgtc
     1201 cgcaagcctt ctgaagcccc atacaccccg gcagccagcc cggctggccc ggccccgata
     1261 atcaccagat cgtaaaatgg catttcagct tctttgcgca atccgatctt ttgggcgact
     1321 tcggcttcgg tcggagcggt gaacaccgta ccatcgggaa atgaaaccag gggaaaggtc
     1381 ggattggtca accttgacac cagaaccagt gattgggcat ccaggtttga ttcgacatcc
     1441 agccagcgga agggaacttg attacgggcc agaaattctt ttaagcggtg cgtttccggg
     1501 ctccagcgat gaccaatgag gcggatgcct tcaaatggag gctggtatcc gctcgaccag
     1561 tcgtcaagta aatctgtcaa aaccgggtag aggtgctcgt cgggtgggtc ccagggtttc
     1621 atcagg
//
LOCUS       DAOQDO010001706         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83467_length_1625_cov_3.218471, whole genome shotgun sequence.
ACCESSION   DAOQDO010001706 DAOQDO010000000
VERSION     DAOQDO010001706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83467_length_1625_cov_3.218471"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKZ53_21965"
     CDS             <1..542
                     /locus_tag="PKZ53_21965"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ43166.1"
                     /translation="APGLESDSPLDFDVVDVSSSPEEVVEALMSEFEAEDVPTLEDEI
                     ETAALPEVFPEPVAEVSAMTEFLDEAEDDLTSEAAPLALEDSLAPPPVVETINTDPFA
                     NSLFEVPIESNLAVCMECGSPATDGDIFCRICNTALPGTTGSFEAIRVESVSVMVCRH
                     CGAGINHGDVFCLKCGEVI"
     gene            716..1081
                     /locus_tag="PKZ53_21970"
     CDS             716..1081
                     /locus_tag="PKZ53_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43167.1"
                     /translation="MSKPIIAVGTDLLFSSKINEASTQTGIKVEFARRPEDVLAKVRA
                     FHPAWLLIDLNSQRVNALDVISGVRAEAELDSIQILGFISHIQKELRQAALQAGCSKV
                     IPRSEFSARLPELLLELSV"
     gene            1131..1595
                     /locus_tag="PKZ53_21975"
     CDS             1131..1595
                     /locus_tag="PKZ53_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNJ43168.1"
                     /translation="MKEEMEVFHRHLQKAKLKRTTQRDLILEVFLETEKHLSVEELYD
                     LVKQRDPSVGFTTVYRTMHLLVEAKLARAVEFNDGRTRYEHEYKHDHHDHLICTECSA
                     LIEFFSPEIERLQEEIAREHSFLIQSHSHRLFGVCRAFHNQEAELPPYCLKR"
BASE COUNT          401 a          407 c          415 g          402 t
ORIGIN      
        1 ctgcgccggg tctggagtct gattcaccac ttgattttga cgtcgtggat gtttcgtcct
       61 cacctgagga agtggtggaa gccctgatgt ctgaatttga agccgaagac gtgcccacac
      121 tcgaagacga aatcgagacc gctgctctgc ccgaggtttt tcccgaacca gttgccgaag
      181 tttcggcaat gaccgagttt ttggacgagg cagaagatga tttaaccagc gaggccgcgc
      241 cactggcact tgaagattct ctcgctccac cgcctgtggt ggagacgatc aacaccgatc
      301 cgtttgccaa ctcgctgttt gaagtcccca ttgagtccaa tctggcggtg tgtatggaat
      361 gtgggtcgcc agcgacggac ggtgatattt tctgccgtat ctgcaatacc gccttacccg
      421 gcaccaccgg gtcatttgaa gccatcaggg tggaatcggt atcagtgatg gtttgtcgtc
      481 attgcggcgc cggaatcaat cacggcgacg ttttttgtct caagtgcggt gaggtgattt
      541 gacgaagtcc gattggtggc gatagctttc cagaaaaaga aacctggctg ggatcaccga
      601 cattgtgctc gtgtcaatcg cttgatcttc aatcagttgc cggcaggatg ctggcgctcc
      661 caggcaatat tgatctgaaa agctatctcc cagcctcagg ctgcgtctgg agtctatgtc
      721 aaaaccaatc attgctgttg gaaccgatct acttttttcg tccaaaatta acgaagccag
      781 tacccaaacc ggtatcaagg tcgaatttgc ccgtcgtcca gaagatgttt tggccaaagt
      841 cagagcgttc catcccgcct ggttactcat tgatctcaat agccaaagag taaatgcgct
      901 ggacgtaatt tcaggcgtac gagccgaagc tgaactcgat tcaattcaaa tcctgggttt
      961 tatttcacac attcaaaagg agttacgcca ggcagccctg caggccggtt gttcaaaagt
     1021 catcccccgg tctgaatttt ccgcccgact tcctgaatta ttgctggagt tgagtgtgtg
     1081 atgaggttga ctggatgccg ccgccactcc cccagaatag aggtttgtgc atgaaagaag
     1141 aaatggaagt gtttcatcgt cacctccaga aagccaaact caagcgtacc acacagcgcg
     1201 atttgatttt ggaagtgttt ctggaaacgg aaaaacacct cagcgtcgaa gaactatatg
     1261 atctggtcaa gcagcgggat ccttccgttg gatttaccac tgtgtaccga accatgcatc
     1321 ttctggttga agccaaactg gcacgtgcgg ttgaattcaa cgatgggcgg acacggtacg
     1381 aacacgagta caagcacgac catcatgatc atctgatttg tactgagtgc agcgcgctga
     1441 tcgaattttt cagccctgag atcgaacgac ttcaggaaga aatcgcccgc gaacactctt
     1501 ttctgatcca aagccacagc caccgactct ttggtgtgtg ccgcgctttt cacaaccagg
     1561 aagccgaact tccaccctat tgcctgaaaa ggtgaagaat gaagaatgaa gaattgagaa
     1621 tgaag
//
LOCUS       DAOQDO010001707         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83579_length_1624_cov_3.233907, whole genome shotgun sequence.
ACCESSION   DAOQDO010001707 DAOQDO010000000
VERSION     DAOQDO010001707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83579_length_1624_cov_3.233907"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..1283
                     /locus_tag="PKZ53_21980"
     CDS             579..1283
                     /locus_tag="PKZ53_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNJ43169.1"
                     /translation="MLDAIHLRLREAANPEKAKILQRFFKTGPGQYGAGDLFLGLTTP
                     FLRQVVREFQTLSVDDTLKLLASEIHEERMLALLIWVRQFEKGDAQTKKTIYDTYLQN
                     TSRINNWDLVDASAASIVGGWLFEQTDRSRLTELAVSESLWERRIAMIATFYFIRRNH
                     FDDTLTIAVQLLSDREDLIHKAVGWMLREMGKRDLAILEGFLQKHYCVMPRTMLRYAI
                     ERFPEDRRQQYLKGTL"
     gene            1307..>1624
                     /locus_tag="PKZ53_21985"
     CDS             1307..>1624
                     /locus_tag="PKZ53_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ43170.1"
                     /translation="MSETPQIELGKIGWIDLTIEDADGVRDFYKSVVGWETSDISMGD
                     YNDYCMHPPGSAPVAGVCHARGTNVGLPAQWLIYITVADLEQSIARCVELGGAVLVAP
                     KSAG"
BASE COUNT          396 a          409 c          435 g          384 t
ORIGIN      
        1 gtatcaaacc gggcatcctg agccaaaacg gtttttggag cgaggctgcg ggcttcgaca
       61 ctgtgcatcc aggtgccgag cagccatccg ctggtcagaa agaaaacacc agttccaatt
      121 aaggcaaaat aagagcgagt cgtgttgtgc tgcataggag aattgagggt ttagagggtt
      181 cagggttcag ggttttaagc atcgagataa gggttttagg cccagggtcg tcgtgtaata
      241 gccgtggtgc gaagcccacg gtcacggcca gatcgaggtt taagcccagc aggggcgtcg
      301 taaaatgact gcaacccgca ggctatattt tcctatcttg gcacatatgg ggttcagggt
      361 ttgggaaatt ctaacctgac cagacacggc agaatatatg tttttgggaa attgttgagt
      421 tgaaagaatc ctatacattg gctcgtcagg caatccagtg aaagaaagca agtcttcagc
      481 aagggtatga cttgcaccat accggtcact tcagtattgt ttggcgcacc ttccgccgtc
      541 caggctaacc gtcacatccc aacggagttc caaaaatctt gctcgacgcc attcacctca
      601 ggctcaggga agctgccaac cctgagaaag ccaaaatttt gcagcggttt ttcaaaaccg
      661 gccccggcca atacggtgcc ggagacctgt ttttgggact caccacgcct tttttgcgtc
      721 aggtcgtgcg tgaattccag acgctttccg tggatgacac cttgaaactt ctggcttctg
      781 aaattcacga agagcgtatg ctcgccttgc tcatctgggt gcgtcagttt gaaaaaggcg
      841 atgctcagac gaaaaaaacg atctatgaca cctacctgca aaataccagc cggatcaata
      901 actgggatct ggtggatgct tcggctgcgt ctatcgtcgg tggctggctg tttgaacaaa
      961 ctgaccgttc gcgcctgact gaactggcgg tgtcggaaag cctttgggaa cggcgcattg
     1021 ccatgattgc cacgttttac ttcatccggc gcaatcactt tgacgacacg ctcacgattg
     1081 cggtgcaact cctttctgat cgcgaagacc tcatccacaa ggctgtgggc tggatgcttc
     1141 gcgaaatggg caagcgagac cttgcaattt tggaagggtt ccttcaaaaa cattactgcg
     1201 ttatgccacg tacaatgctt cggtatgcca tcgagcgctt ccccgaagat cggcggcagc
     1261 aatatctcaa aggaactctt taaccaacac agaaaggaac tcacgtatga gcgaaactcc
     1321 acagattgaa ctcggtaaaa ttggatggat tgacctgacc attgaggatg ccgacggtgt
     1381 ccgtgatttt tataaatcag ttgtcggctg ggaaaccagt gacatcagca tgggtgacta
     1441 taacgactat tgcatgcatc cgccgggaag tgccccggtt gcaggtgttt gccacgcacg
     1501 cggcaccaac gttggattgc ccgcgcaatg gctgatttac atcacggtgg ccgatctcga
     1561 acagagcatc gcccgctgtg ttgaactcgg cggtgccgtt ctcgtcgccc cgaagtcagc
     1621 cggc
//
LOCUS       DAOQDO010001708         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83672_length_1623_cov_4.191964, whole genome shotgun sequence.
ACCESSION   DAOQDO010001708 DAOQDO010000000
VERSION     DAOQDO010001708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83672_length_1623_cov_4.191964"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..423)
                     /locus_tag="PKZ53_21990"
     CDS             complement(73..423)
                     /locus_tag="PKZ53_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43171.1"
                     /translation="MNFFLVEGATDPAGYPMADLISLVEFDRRDQNLRQFLIFSLGVM
                     GVAFVVFVILIKWVWTVLPIIIVVHVLVLTGFVTISRRVGQCPQCRTSLKVEIHDYHP
                     RPVVFCEDCHIRYR"
     gene            902..1537
                     /locus_tag="PKZ53_21995"
     CDS             902..1537
                     /locus_tag="PKZ53_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43172.1"
                     /translation="MDSYKRFVKKRNRKIQNSVVGGMIFSVVYIGFPTGSFLLGLSNL
                     QTSLEPVLIVSTLLSLLIACFFGWMFGTFFRTVHHFRVVAYYNRRVDDPLLDTWLTGK
                     AIFHNLKDLDDLCDQAGIQRMTEFGFLDDLDGDKMIWHNPLDGLVTIRTLINQLYTEE
                     SDTTPLLSELEKIEYALTQAASKDIQFAFILRPVNGGTSGQEMEIRQGTFW"
BASE COUNT          424 a          369 c          386 g          444 t
ORIGIN      
        1 acgaatcaaa ataatttggg taatgacaag acccggaacc cggaacccgg aacccggtat
       61 tttcttcatt cttcaccggt agcgaatgtg acaatcttca caaaagacaa ctggtcgcgg
      121 gtgatagtcg tggatttcga ctttcagact ggttcgacat tgtgggcatt gaccaacccg
      181 acgtgaaata gtgacgaatc cagttaaaac cagaacatgc accacaataa tgattgggag
      241 aacagtccaa acccatttga tcaagatgac gaacaccaca aaagcaaccc ccataactcc
      301 caacgaaaaa atcagaaact ggcgcaggtt ttgatctcgc cggtcaaact ccacaagtga
      361 tatcaggtca gccatcgggt agcccgccgg gtcagttgca ccttcaacca aaaagaaatt
      421 cattttttct cccccttcac aggtgggttg aagtattggc ccggattgaa taacgaccag
      481 gggtgaacat cgttggcact ctccggtttc ctgatcaaac agatcggact ggtgacaaac
      541 ctcacatcgt ttgggaaggt gttgttgctt gacacgaatc atacgctcaa tacctgtggt
      601 ttcaggactt agggcttgag gggatgcatc attctcggga aaaagaggga ttttcgcagt
      661 aagcccttat cctacacgca attctgattc gtgcaattct gcattgaaat tttgccggga
      721 aattccagtt ttttgccggt ttacaacggc accaggtggg ttaaatcttc ctggctcaaa
      781 tcagatcaga gtggccagta catcatttga gtgctatggt ttgcagtgta ttcgatgctt
      841 tgcaaccgct ctgtatttga tccgttacca atccgggctt cctgactgag agctatagtt
      901 tatggattca tataaacggt ttgtcaaaaa acgaaatcga aaaatccaga attcagttgt
      961 ggggggcatg attttctcgg ttgtttatat tggttttcca actggatcat ttcttttggg
     1021 tctcagtaac ctgcaaacca gcctggagcc agtactgatt gtctcaaccc tgctcagtct
     1081 gttgatcgcc tgcttttttg ggtggatgtt tggaacattc ttccgcaccg ttcaccactt
     1141 tcgagttgta gcctattaca atcgcagggt tgatgaccca ttgcttgata cctggttgac
     1201 tggtaaagcc atttttcaca acctgaaaga ccttgatgac ctgtgcgatc aggctggcat
     1261 tcaacgaatg accgaatttg ggtttttgga tgatttggac ggggacaaaa tgatctggca
     1321 caatccgctg gatggcctgg tcaccatccg aacgctgatc aaccaacttt ataccgagga
     1381 atccgacaca actcccctgt tgagtgaact tgagaaaatt gaatatgcac tcacccaggc
     1441 agcttcaaaa gatattcaat ttgccttcat cttgcgcccg gtaaatggcg gcacctccgg
     1501 ccaggaaatg gaaatcagac aggggacgtt ttggtagctc caacttgggg aaagttggaa
     1561 atgaagaatt gaattggtgc ttggtgcttg gtgcttggtt cttggtgctt ggtgcttggt
     1621 gct
//
LOCUS       DAOQDO010001709         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83678_length_1623_cov_3.536352, whole genome shotgun sequence.
ACCESSION   DAOQDO010001709 DAOQDO010000000
VERSION     DAOQDO010001709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83678_length_1623_cov_3.536352"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1623)
                     /locus_tag="PKZ53_22000"
     CDS             complement(119..>1623)
                     /locus_tag="PKZ53_22000"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNJ43173.1"
                     /translation="SLLRMPGGCFEQTSSTTYPNVLVMDYLKTTKKIAPEIQVKAEGF
                     ISLGYQRLVTFEVNGGGVSWFGEAPANKILTSYGLMEFYDMSKVHEVDPRLISRTQQW
                     LASQQQPDGSFKPDTSFINEGATNQFNTDVVRITAYIGWALAYTGYQGEAIDKAKRYV
                     ESHTTGKEDAYTEAVIANFAADYKADKTWADRTVGNLVESATQQNDMMFWEVGGKTPT
                     YASGDNAAIETTALATQAVIKWGRASDVARKALAYLTSKKDSFGNWQSTQATILSLKA
                     FLLAQKAASSGDTRGTVEVVVNGQPVHRFTIDADNQDLMQQVDLKRWTQTSGAHQVDL
                     RFSGQGSLLYQIVGRYYMPWAARKTSDGAGAEPLSIKVDYDRTRLAQDDIATAKVTVR
                     NNQNLNAELVMVDLGIPPGFEPLAEDLQELMGMNAGQQGGHVTKFTITGKQVILYFDA
                     FKARQTVSFNFRLKAKYPLKAKTVSSQVYEYYNPQKGGNAVPVEMVVTSK"
BASE COUNT          327 a          453 c          413 g          430 t
ORIGIN      
        1 gactcgcaag ctcggggcgg aagaaaccgg gtttaatacc ctgagcccaa tttcttcagc
       61 ccgatgtctt cagcccctag ccctcaaccc tcagccctgg ttttctcagc ccgctgtctt
      121 actttgaagt caccaccatt tcaaccggca cggcgtttcc gcctttctgc gggttgtaat
      181 attcatacac ttgcgacgaa acggtcttgg ctttgagcgg atacttcgct ttcaatctga
      241 aattgaaact caccgtttgc cgggctttga aggcgtcgaa gtacaaaata acctgtttgc
      301 cggtaatcgt gaacttcgta acgtggccgc cttgttgacc ggcgttcatg cccatcagtt
      361 cctggaggtc ttcggccagc ggttcaaatc caggaggaat ccccaaatcc accattacca
      421 gttcggcatt caaattctga ttgttacgaa ccgttacttt cgcggtggcg atgtcgtctt
      481 gtgccagtcg cgtgcggtca tagtcaacct tgatgctgag cggttcggca ccggcgccgt
      541 cgctggtttt tctcgctgcc cacggcatgt aataccgtcc gacgatttga tagagtagac
      601 tcccctgacc actaaaccgc aggtcaacct gatgtgcccc ggaggtttgc gtccagcgtt
      661 tgaggtcaac ctgctgcatc aggtcctggt tgtcggcgtc aatcgtgaag cgatgcaccg
      721 gctgaccatt gaccacaact tcgaccgttc cgcgagtgtc acccgaagat gctgcttttt
      781 gggccagcaa aaatgccttg agcgacaaaa tcgtggcctg ggtggattgc cagttgccga
      841 atgaatcttt cttgctcgtc aaataggcca gggctttgcg ggcgacatca ctggcgcgtc
      901 cccacttgat gacggcttgc gttgccagcg cggtggtttc aatcgcggca ttgtctccgc
      961 tggcgtaggt cggagttttt ccgccgactt cccaaaacat catgtcattt tgttgggtgg
     1021 ccgattcgac cagatttcca accgttcgat ctgcccaggt tttgtcggct ttgtaatcgg
     1081 cagcgaagtt ggcaatcacg gcttcggtat aggcgtcttc cttgcccgtt gtgtggcttt
     1141 cgacatagcg tttggcttta tcaatcgcct ctccctgata tccggtgtaa gccagcgccc
     1201 atccgatata ggccgtaatg cgcaccacat ccgtgttgaa ttgattggtc gcaccttcgt
     1261 tgataaatga cgtgtccggt ttgaaactgc catccggttg ctgttgcgaa gccagccact
     1321 gttgcgttcg tgaaatcaaa cgcgggtcaa cttcgtgaac tttcgacatg tcgtaaaatt
     1381 ccatcaaccc gtaggaagtc aaaatcttgt tggccggcgc ttcgccaaac catgaaaccc
     1441 caccgccatt gacttcaaac gtcaccaacc gctgatagcc cagggaaata aagccttcgg
     1501 ctttgacctg gatttcaggc gcgattttct tcgtggtttt cagataatcc atcaccagca
     1561 cgtttggata ggtcgtggag gatgtttgtt caaagcaccc gccgggcatg cggagcaagc
     1621 tgt
//
LOCUS       DAOQDO010001710         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83685_length_1623_cov_3.119898, whole genome shotgun sequence.
ACCESSION   DAOQDO010001710 DAOQDO010000000
VERSION     DAOQDO010001710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83685_length_1623_cov_3.119898"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PKZ53_22005"
     CDS             <1..1354
                     /locus_tag="PKZ53_22005"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ43174.1"
                     /translation="TKTYGRVTETILDTPLPVPIELEPVFDAKTEATLSKLTPATGIE
                     KIDSVEMAFWDSIRSSTNPKEFAAYLKRYPKGQFAELAEIKLEQLTSDQGLTSGSTFG
                     VVPTPVYRPIQGTPPPDASPAPISALHTPLPSQPPSVVQPGPGVSSASHPVVPPTQPL
                     VPPTQPLVPPTQPLPQVPPIQPRYPTGPQTQLTLPPSRPPVSTMQVQTEIKFTNQLGM
                     DLVMIGVGSFLMGSTEVENEGPVRQVSIRRPFYIGKYQVTQKQWLAVMGQNPSNFSGD
                     NLPVEQVSWEAAVEFLRRLNASGGKFLYRLPTEAEWEYACRAGTTTRYSFGDDEKLLG
                     EYAWYDDNSAVDDGFSEDIAESSTQPVGKKKPNAWGLHDMHGNVWEWCLDWYHDTYQG
                     APPDNRPWDDGTSHQYRVVRGGSWLDSARYCRSARRRNVAPQIGLSAVGFRVVAAIRM
                     "
BASE COUNT          379 a          461 c          432 g          351 t
ORIGIN      
        1 taccaaaacc tatggtcggg tcactgaaac cattttggac accccgctcc ccgtgccgat
       61 agaactggag ccggtctttg atgctaaaac agaggccacg ctgtcaaaac tgacacccgc
      121 cacggggatt gaaaagattg actcagttga aatggccttt tgggattcaa tccgcagcag
      181 caccaatccc aaagaatttg ctgcctacct caaacgctac ccgaaaggac aatttgccga
      241 actggccgag atcaagcttg agcaactcac cagtgatcag gggctgactt caggttccac
      301 gtttggggtg gtgcccacgc cggtctatcg tccaatccag ggaaccccgc cgccggatgc
      361 ttcgccggcg ccgatatcgg ccttacacac cccacttccc agtcaaccac cctcagtggt
      421 ccagcctgga cctggcgttt cttcggccag tcatccggtg gtgccgccaa ctcagccgct
      481 ggtgccgcca acccagccgt tggtaccacc tacccaaccc cttccgcagg tgccaccgat
      541 acagccgaga tatccgaccg gaccccaaac ccaattgacg ttgccaccct cgcgaccacc
      601 cgtttcaacc atgcaggttc aaacggagat taaatttacc aatcaacttg gcatggatct
      661 ggtcatgatc ggcgtcggat cgtttttgat ggggtcaacc gaagtcgaaa acgaagggcc
      721 ggttcgtcag gtatctatcc gccgcccatt ttatataggg aaatatcagg tgacccaaaa
      781 acaatggttg gccgtgatgg ggcaaaaccc aagtaacttc agcggtgaca atctgcctgt
      841 tgagcaggtt tcgtgggaag ctgccgtgga gtttctccgg cgactcaatg ccagtggagg
      901 aaaatttctg taccgcctcc ccaccgaagc cgaatgggaa tatgcctgcc gggctgggac
      961 caccacacgc tactcgtttg gcgatgatga aaaactgctt ggcgaatatg cctggtatga
     1021 cgataactca gccgtggacg atggattcag cgaagatatt gctgagagca gcacccagcc
     1081 cgtcggtaaa aagaagccca atgcctgggg gttgcacgac atgcacggca atgtctggga
     1141 gtggtgcctc gactggtatc acgacaccta ccagggtgcc ccacccgata accggccctg
     1201 ggatgacggc accagccatc aataccgcgt tgttcgcgga ggctcgtggc tcgatagtgc
     1261 ccgctattgt cggtccgccc ggcgacgcaa tgttgcaccc cagatcggcc tgagtgcggt
     1321 tgggttccgg gttgtggctg ccatccggat gtaaccagga atcagcctgg gtcaggcatc
     1381 aaactgcaaa attcaatgag ttatgctcac ctgagtttcc ccacttaaag cctttgatta
     1441 caagtgatta aatgactgtc aacgcgttga cactttcatt tctggccgaa taggggtgac
     1501 ctataaaaaa tgttgaatcg cagcttcatc ggctggtttt tgaaggggtg gaagagaagt
     1561 gaccaggctg gtggtggtgg tccgtgcttt atttttttca agcaggcgag tggccagcac
     1621 cat
//
LOCUS       DAOQDO010001711         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_83960_length_1620_cov_3.741214, whole genome shotgun sequence.
ACCESSION   DAOQDO010001711 DAOQDO010000000
VERSION     DAOQDO010001711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83960_length_1620_cov_3.741214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PKZ53_22010"
     CDS             complement(<1..1295)
                     /locus_tag="PKZ53_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43175.1"
                     /translation="MPNQRQFFIAVILALWIGIGFAIPEYPTNARSDLNPESPRTQHT
                     DPQGAGLPQLSKGDSIILSAKDQLRAGQMQELAELERQLIDLKTLPQHQWSPAQSRQY
                     ATLKTRIEPLRNTLYGSQPPDPQSVFVTKGFIPANDGCVPANHTITHSLSQTVLPGFG
                     VSYVDGETISHSANSYVRAFDLATDFGITTDFDITSIDFGIQVADAANDDLQPLTVNL
                     FQTCGAFPVSLSTLDLIGTTTINLPDQALTVVNIPVNGTVSVGATLIVEVSIPDATDI
                     GDIFFPGANNLGQTGPTYLASRGFTDIPFDLADSFFPDSHDVMNVHGLTVCDQGTECT
                     NPGRLLLVADTVNNRIQAFDGLDWTAPLMTLGSAVGQVRAPKSITQTSDGRVIYVADT
                     GNNRIQKTLNGGCSWDVVALTGVLPATLRSPGGIAVKPN"
BASE COUNT          416 a          364 c          423 g          417 t
ORIGIN      
        1 tcatttggtt tgacggcaat gccgcctgga cttctgagcg tggcaggtag aacaccggtc
       61 aacgcaacta catcccagga gcacccgcca ttcagggttt tttgaatccg gttgtttccg
      121 gtgtcggcta cgtaaattac ccgcccgtcg ctggtttggg ttatgctctt tggagcgcgc
      181 acctgaccga ccgctgaccc gagggtcatc aacggtgctg tccaatccaa tccgtcaaaa
      241 gcctgaatcc gattgttgac ggtatccgcc accagcagca atctgcctgg attggtgcac
      301 tcggtgccct gatcacacac ggttaagccg tggacgttca tgacatcatg actgtctggg
      361 aaaaatgagt ctgccaaatc aaatggaata tcagtaaatc ctcttgatgc aagataggtt
      421 ggaccggtct gacctaaatt attggcccct ggaaagaaga tgtctccgat gtcggtggca
      481 tccgggatgg atacctcgac aatcaaggtg gccccgactg acacggtgcc attgaccggg
      541 atattgacca ccgttaatgc ctggtccgga agattgatgg tggtggttcc aatcaaatcc
      601 agggtggaaa ggctcactgg aaaggctccg caggtttgaa agagattcac cgtcaacggt
      661 tgcaggtcat cattcgctgc atcagcgacc tgaatgccaa aatcaatact ggtaatgtca
      721 aaatccgttg tgattccaaa gtcggttgcc aggtcaaagg cgcgaacata gctattcgca
      781 ctatggctga tggtttcccc atcaacataa gaaaccccga atccgggcag caccgtttgg
      841 ctgagggaat gggtgatggt gtggttggct ggaacacacc catcatttgc cggaatgaat
      901 cctttggtga cgaaaacgct ttgggggtct ggtggctggg aaccatacag tgtattccga
      961 agcggttcta tccgggtttt gagagtggca tattgtctgg attgggccgg agaccattga
     1021 tgttgaggga gcgtcttcaa gtcaatgagt tgcctttcca gctctgccaa ttcctgcatc
     1081 tggccggctc gaagctgatc cttcgccgac aaaataatgg aatcgccttt ggataactgt
     1141 gggagtccgg ccccttgtgg atcggtatgc tgggttcggg gagattccgg gttcagatcg
     1201 gatctggcat tggtgggata ctctgggatt gcaaagccaa taccaatcca cagagcaagg
     1261 attactgcga taaaaaattg tctttggttg ggcatgtttt ttcctcgaat tctctgaaaa
     1321 ctgggattcg ctctttcctc ataagctatt gaagataaac aagttttgat ctaatccggt
     1381 tcgattgctt tagaaatgca aaattttgaa ttacttacat ttacagtttt gaaagaaact
     1441 taaattataa aaagagcgaa actccaattc atacataaaa gcgtaaaatt tcaaaaaaag
     1501 gggtcacgta ttctttaatt caagataaca caaatactcg gcaggtgagg gtttgggttg
     1561 aggtagcgaa atgagattat cggaagacgc tcaaaacaag ctgaaggtcc tgacagccct
//
LOCUS       DAOQDO010001712         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84095_length_1619_cov_2.950767, whole genome shotgun sequence.
ACCESSION   DAOQDO010001712 DAOQDO010000000
VERSION     DAOQDO010001712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84095_length_1619_cov_2.950767"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1619
                     /locus_tag="PKZ53_22015"
     CDS             <1..>1619
                     /locus_tag="PKZ53_22015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ43176.1"
                     /translation="TPPYKQYQFGATVGGPIKKDKLFYFGSFERLSVKQNNIVTIPES
                     IVQSARRIGFSYVDNGAQPFALGNTSVLARTDMQVNANNLLTLRYNYGGTFDGNFEPF
                     GALTDRSNSGTQRLNDNQFAASNTYINANLNMVNETRFLYSHRSQDVLTDTVGPRVSI
                     NSGDVGRIIFGPPIFLNQPRRVNIFQIVDNVSLSRGNHQIKFGVDFLRIMAKNRLPSR
                     EQGSITFNDLDLRFIGLPGDGFFRAIEAFDPSLRSADQKTFLSALAPLVPLFANSPNF
                     PDNLDLANLPLPTFYAQGFISQKPLEVAQNFFSAFIQDDIKLRDNLMLKLGLRYDRFT
                     TQFLDGNRGDFSPRVALSYTPSKFPKLSLYATYGLFFATPNIGPIFAAFPKGRSTSFQ
                     LLPETILTFQRPTRNFPVSDQIPENVGFARQFGLEDQVQQDLQTSYTQQTGLGFNYFI
                     TPKTILNISYNYTRGIRLFGTRDVNPVVRPEPILAQSYLNGRVDTTRGAVLYYESAFD
                     SYFHGLSTSINRRFANNFGFQASYTFSKTID"
BASE COUNT          402 a          430 c          354 g          433 t
ORIGIN      
        1 aaaccccgcc gtataaacaa taccagtttg gggcaacggt cggcggcccg atcaagaaag
       61 ataaactctt ttattttggc tcatttgagc gtctttcagt caaacaaaac aacattgtca
      121 ccattcctga aagtattgtt caatcagccc gccggatcgg gttttcctat gttgacaacg
      181 gggctcaacc atttgccctc ggaaacactt ctgtcctggc cagaaccgat atgcaggtca
      241 atgccaacaa cctgctgacc ctgcgctaca actatggcgg gacgttcgat ggtaactttg
      301 agccgtttgg ggcgttaact gaccgatcca actcgggaac tcaacggctc aacgacaatc
      361 agtttgctgc cagcaatacc tatatcaacg ccaacctcaa tatggtcaat gaaacccggt
      421 ttctctatag ccatcggtct caagatgtgc tgactgacac ggtggggcct cgggtttcaa
      481 tcaatagcgg cgatgtgggt cgcatcatct tcggaccacc aatctttctg aatcagccac
      541 ggcgggtcaa tatttttcag attgtggata acgtctcact gtctcggggc aatcatcaaa
      601 ttaaatttgg ggtcgatttc ctgcggatta tggccaaaaa ccgccttccg agccgtgaac
      661 agggttcaat cacgtttaat gacctggatt tgcgatttat tggcttgccc ggagatggat
      721 tctttcgggc gattgaggcg tttgatccca gcctgcggtc agcggatcaa aagacatttt
      781 tgagtgcgct ggcgccgctg gtgccactgt ttgccaattc tccaaatttc ccagacaacc
      841 tggatctggc aaacctgccg cttccgactt tttatgccca ggggtttatt tctcaaaaac
      901 cactggaagt cgcccaaaac ttcttttctg cctttattca agatgacatc aaattacgtg
      961 ataatctgat gctcaagctg ggtctgcgct atgaccggtt tacaacacag tttttggatg
     1021 gcaatcgagg agacttcagt ccgcgagtgg cattgtcata taccccgagt aaattcccga
     1081 aactttcgct gtacgccacc tatgggttgt tttttgcaac cccaaatatc ggccccattt
     1141 ttgcggcctt cccgaaaggc cgcagcacct cattccaatt gctgccagaa accattttga
     1201 ccttccagcg accaacccga aattttccag tctcggatca gattcctgaa aacgtagggt
     1261 ttgcccgcca atttggcctt gaagatcagg ttcaacagga tctccaaacc agctataccc
     1321 agcaaacagg gttggggttt aattacttca ttaccccaaa gacgattctg aatatctcct
     1381 ataactacac ccgcggtatt cgtctgtttg ggacccgtga tgtcaaccca gtggtccgac
     1441 cagaacccat tctggctcaa agttatctca atggccgggt tgataccacc cggggagcag
     1501 ttctgtacta cgaatcagct tttgatagct actttcatgg actatcaacc tcaatcaatc
     1561 gccgatttgc aaataacttt gggttccagg ccagctacac attttcaaaa acgattgat
//
LOCUS       DAOQDO010001713         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84289_length_1617_cov_3.576825, whole genome shotgun sequence.
ACCESSION   DAOQDO010001713 DAOQDO010000000
VERSION     DAOQDO010001713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84289_length_1617_cov_3.576825"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..598)
                     /locus_tag="PKZ53_22020"
     CDS             complement(215..598)
                     /locus_tag="PKZ53_22020"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ43177.1"
                     /translation="MNRSAKLLVVLFISVAFIAANQLNSLALSPVKVSPAATVTSAED
                     AKSGAKLFAETCKICHHTESTDSLVGPGLKGLYKDRKKMLSGKAVTDDNVKNLIQKGR
                     NAMPAYGSKFSAAQLADLIAYLKTL"
     gene            669..1508
                     /locus_tag="PKZ53_22025"
     CDS             669..1508
                     /locus_tag="PKZ53_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43178.1"
                     /translation="MEKNTLQRWKWQALPDLFQGSAHASYQLADRVLDWGLNLAAPQV
                     CKICGKLVLSRHEGVTCKACWSDYGHTGAAYQVSCLRCGRWRARPTERDVTEVQSNCD
                     HCQEMVFTQARSCGPYLGALRENLLLSKRHPYLPKRLRSYISTAWAHYPELHQANRIL
                     PVPLARGRLRERGHNQAELIARLLATQSGIELDSFTLARIAETERHRVGMDAHRRAKS
                     VQRAFQVICPQLIENTSVLLVDDVFTTGATIEACSRILLTAGATAVMVFTVGRTMRYS
                     DLI"
BASE COUNT          411 a          396 c          428 g          382 t
ORIGIN      
        1 ccaactgggt aaatttttca actcatcaag cgacagtttg aggcgcggac tcgacatggt
       61 gggaaggatc tcctttggaa tgagcgaagt agtaaagtct gaacgagcga aggagcgaac
      121 cagcgaatag tggacgagcg aggtcgtgaa ttctttgtct tggcactctt tcgctctttc
      181 gccagttcac tccactggct ctttcactca aaaatcagag cgttttcaaa taagcaatca
      241 ggtcagccag ttgtgcggca ctaaatttgc tcccgtaggc gggcatggcg ttgcggccct
      301 tttgaattaa atttttgaca ttgtcatcgg tcacggcttt tccactcagc attttcttgc
      361 ggtctttata gagtcctttt agcccaggac caaccagcga gtcagtgctc tcggtgtggt
      421 gacagatttt gcaggtttcg gcaaagagtt tggcgccgct tttggcatct tcagcgcttg
      481 tgacagtagc tgccggtgaa actttaacag gtgacaaggc cagtgaattc aactggtttg
      541 cagcaataaa ggcaactgaa ataaacaaca cgaccagtaa tttggcagat cggttcatac
      601 aattctccaa tgtgaacaaa aagaataagg tgggtgcgat aggtcagatt aaacgaaggg
      661 ataaaaggat ggaaaaaaat accttgcagc ggtggaagtg gcaagccctt cctgatttgt
      721 tccagggttc ggctcacgcc agttatcaac tggcagatcg cgtactcgac tgggggttaa
      781 atctggcggc accacaggta tgcaaaatct gcggaaaact ggtcctgtca cgtcacgaag
      841 gagttacctg taaggcgtgc tggtcagatt atggacatac aggtgccgcc tatcaggtga
      901 gttgtcttcg atgtggtcgg tggcgtgcaa gacccaccga acgggacgtg actgaagttc
      961 aatcgaattg cgaccactgc caggaaatgg tgttcactca ggcgcggtcg tgtgggccat
     1021 acctcggagc actccgtgag aatctgctgc tttccaagcg tcatccatat ctgcccaaac
     1081 gacttcgaag ctatatttcc accgcctggg cacactatcc ggaactccat caggcaaacc
     1141 ggattcttcc ggttccgctg gcccgtggga gactccgcga acgtggtcac aaccaggccg
     1201 aattgatcgc ccgccttctg gcaacccaat ccgggattga gcttgattcc tttactttag
     1261 ctcgaattgc cgaaaccgaa cgtcaccggg ttgggatgga tgcccaccgc cgcgccaaaa
     1321 gcgtgcagcg cgcattccag gttatctgcc cacaactgat agaaaatact tcagttttgc
     1381 tggtggacga tgtatttacg actggtgcca ccattgaagc ctgttccaga atactgctga
     1441 ctgccggcgc cacagcagta atggttttca ccgttggccg gaccatgcgg tatagcgatt
     1501 taatttaatt tgggttcagg agtcgggggt agaaaggaca taaaggacga aaaggacgaa
     1561 aaagacataa attcgaaaac ccgaaacccg gaacccggaa cccggaaccc ggaaccc
//
LOCUS       DAOQDO010001714         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84298_length_1617_cov_3.110115, whole genome shotgun sequence.
ACCESSION   DAOQDO010001714 DAOQDO010000000
VERSION     DAOQDO010001714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84298_length_1617_cov_3.110115"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKZ53_22030"
     CDS             <1..317
                     /locus_tag="PKZ53_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43179.1"
                     /translation="LGGLHTTSPARRGGRGAMGAADERAVSQLRAQLESSMRQRQDLE
                     LDANRIREKADETRSRFDQALANIELDPNAVLDVDSEWWIETMKNARVNKEITVHFDD
                     EP"
     gene            322..762
                     /locus_tag="PKZ53_22035"
     CDS             322..762
                     /locus_tag="PKZ53_22035"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNJ43180.1"
                     /translation="MSFLLDTNHCVYLNNAKNKRPEDCKAFEIHTLATFQRVSDPLFV
                     SEVTLGEMYFGAAKSQQAAKNYGRVEALRTSLNVLPVTLGLWKLFGETKADLQKQGKV
                     IADFDLLIGCTAMYHGLTLVTNDKAFEMLKPTLKLVDWSIGTTP"
     gene            792..1079
                     /locus_tag="PKZ53_22040"
     CDS             792..1079
                     /locus_tag="PKZ53_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43181.1"
                     /translation="MKEKPTNEERLIARTFFLEAKQDEWLTRIAQIKKMTKSELLRMA
                     VDDVIEKLATEILAEREREAARQQELERQRAEEEAQRKAKARTPKPKKPKA"
     gene            1119..1316
                     /locus_tag="PKZ53_22045"
     CDS             1119..1316
                     /locus_tag="PKZ53_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43182.1"
                     /translation="MPNEETDNGQILAQQEVVPTIPTQGNRQGDYFSGDAWERATGSP
                     EEIQAVIAGLKKFLAVCSQKD"
BASE COUNT          470 a          383 c          420 g          344 t
ORIGIN      
        1 cactcggcgg cctgcacacc acctcccccg cgcgccgcgg cggtcggggc gcgatgggcg
       61 ccgccgacga gcgggccgtc tcgcagcttc gtgcccagct cgagtcgtcg atgaggcagc
      121 gtcaggacct cgagctcgac gccaaccgca tccgcgagaa ggcggacgag acccgcagcc
      181 gatttgacca ggcactcgcc aatattgagc ttgaccccaa tgctgttttg gatgttgatt
      241 cagagtggtg gattgagacg atgaagaatg cacgggtgaa taaagagata actgtacatt
      301 ttgacgacga gccatagcac tatgtctttt ttgttggata cgaatcattg cgtttacctg
      361 aacaacgcga aaaacaagcg accggaggac tgcaaagctt ttgaaataca cactcttgcc
      421 acgttccaac gtgtgagcga ccctcttttt gtttcggaag ttactctggg tgagatgtat
      481 ttcggtgcgg cgaagtccca gcaagcggcc aaaaactatg gacgggttga ggctttgcga
      541 acaagcctga atgttttacc agtgacactg ggtttgtgga aactttttgg agaaacaaaa
      601 gcagaccttc agaaacaggg aaaggtaatc gctgattttg atctactgat tggatgcacc
      661 gctatgtatc acggcctcac tttggtcacg aatgataaag cctttgagat gctgaaacca
      721 accttgaagc ttgtagactg gtcaatcgga acgacaccct gacattccca cacgaggaga
      781 cccaaccggc aatgaaagag aaaccgacca acgaagaacg cctgatagcc cgcacgtttt
      841 ttctggaagc gaaacaggat gaatggctca cacggattgc gcagatcaag aagatgacca
      901 aaagcgagct tttgcggatg gcggtggatg acgtgatcga gaagctggcc actgaaattt
      961 tggcggaacg ggagcgggaa gccgcccgcc aacaggaact tgaacgccag cgggcggagg
     1021 aagaagccca acggaaagcg aaggccagaa cccccaaacc gaagaaaccc aaagcatgaa
     1081 actcccatcc ggtgacgaaa gaaaagaagc gactaactat gccgaatgaa gaaacagaca
     1141 atggacagat tctggctcaa caggaagttg tgccgaccat ccccacccaa ggcaatagac
     1201 agggagacta tttttcaggc gacgcgtggg aacgggcaac cggcagtcct gaagaaatac
     1261 aagccgtcat tgcaggtttg aagaaatttt tagccgtttg ttctcagaag gactgatgag
     1321 gagcggcaac caagtaactt tcccttcctc aaaaccgata tgaaaaggca attgattgac
     1381 ctttgacata aaaacctgga ggagtttggg cttgaattca agataagtgc aattatcttg
     1441 aattaaactt gattgctatg aatttatgcc aagacgcaat tctataatgg ccctttttaa
     1501 gcccatttta cacttttaca gtaagggtgt ttttgcataa accgacaaaa cggcatattt
     1561 caggctcttt ggtatttccc gtggaaacga acaaaacttc aaaaacgaat attgatt
//
LOCUS       DAOQDO010001715         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84392_length_1616_cov_3.761691, whole genome shotgun sequence.
ACCESSION   DAOQDO010001715 DAOQDO010000000
VERSION     DAOQDO010001715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84392_length_1616_cov_3.761691"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKZ53_22050"
     CDS             complement(<1..536)
                     /locus_tag="PKZ53_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43183.1"
                     /translation="METELFEEAQLDPVLYVSDACGPASPQTINCLAAANRPTGGPFF
                     LENEEIRCLTLTQNQTIYIYVDDANSQSTPFEFTVVVTPCNSEVEPNDDPETPSLVSC
                     NTQGGFSGQDPVDYFSAGMNTIDGSRLFAVVDASTMTGSDLDVRVIQGNDTLEYDDDD
                     NAVELGDQSPNVAGTPLGT"
     gene            728..>1616
                     /locus_tag="PKZ53_22055"
     CDS             728..>1616
                     /locus_tag="PKZ53_22055"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNJ43184.1"
                     /translation="MKALLTSQILRTKYRRGDKDFVRQRLLTLPVVIILILKGHKMSF
                     QNILNKVFEALGLVEKVPTASAYCQAREKLKPEVFVELNRQVVRDYMELSRQDGTLKL
                     WNGYRLLGVDGTYLNLPDTEETRAEYSVQTNQFAGSDQVQALMSVMYDLLNEVGISAG
                     LGKRQGENNFLIQPHLSETEWLDLLVLDRGYANYLTMALIYKYQRDFVIRFPSKSLRE
                     VNAFWTASDTDRVVTVVCPPKAVAEVRELGLPETITFRLVKVELPDGKLEVLATSLLD
                     AVTFPPPALKQVYGWRWNEE"
BASE COUNT          409 a          368 c          462 g          377 t
ORIGIN      
        1 gtgccaagtg gcgttccggc cacattgggt gactgatctc caagctcaac ggcattatcg
       61 tcatcgtcat attccagtgt gtcattaccc tggatgaccc gcacatccag gtcactaccc
      121 gtcatggtgc tggcatccac cacggcaaac aggcggctcc cgtcaatggt attcatccca
      181 gcggagaaat aatcaaccgg gtcttgtcct gaaaatccgc cttgagtgtt acaactcacc
      241 agggaaggtg tttcaggatc gtcatttggc tcgacttcgc tattgcaggg ggtgaccacc
      301 acggtaaact caaatggggt tgattgagaa ttggcatcat ctacataaat gtaaattgtc
      361 tggttctggg ttaacgtcag acagcggatt tcttcatttt ccaggaaaaa cggacctcct
      421 gtgggccgat tggctgccgc caggcaattg atggtttgtg ggctggcggg tccgcaggca
      481 tcggaaacat acagcaccgg gtccaactgg gcttcttcaa ataattctgt ctccaatcgg
      541 accgagtagg tgccagttgc cggcgccgta aacgaataga cgacatctgg tccagccgct
      601 ggatcaggtt gatttccgtc cccttgcagg ccaatggcat taagttaagc aaaaagatgg
      661 acaacccgtt gaaaataata tagctataga aaatgatgat aaaaaaaacg aacttctcat
      721 tgaacgattg aaagccctgt tgacgagcca gatcctgcga accaaatatc gaagagggga
      781 caaagatttt gtgcggcagc gattgttgac actgccagtg gtgatcattc tgattctcaa
      841 aggccataaa atgtcattcc agaatatcct gaacaaagta tttgaggcgc tggggttggt
      901 tgaaaaagtg ccgacggcga gtgcgtattg tcaagccaga gagaaattga agccggaagt
      961 gtttgtggaa ctgaaccggc aagtggtgcg cgattatatg gaattatcgc ggcaggacgg
     1021 gacattgaag ctatggaacg gatatcggtt gttgggggtg gatgggacat atttgaatct
     1081 gccggacacc gaagagacac gcgccgagta tagtgtgcaa accaatcagt ttgccggaag
     1141 cgaccaggtc caggcattga tgtcggtgat gtatgacctg ctcaacgagg tgggaatcag
     1201 tgccggactg gggaagcggc aaggggaaaa caactttttg atccagccgc atctgagcga
     1261 gaccgaatgg ctggatttgc tggtgttgga tcggggatat gccaattatc tgaccatggc
     1321 actcatctat aagtatcagc gcgactttgt gatccggttt ccgagcaaaa gtttgcggga
     1381 agtcaatgcg ttttggacgg catccgacac cgaccgggtg gtgacggtgg tctgtccgcc
     1441 aaaggccgtc gctgaggtgc gggaattggg gttgcccgaa accatcacct ttcggttggt
     1501 gaaagtggaa ctcccggatg gcaaactcga ggttctggcc acgagtttgc tggatgcggt
     1561 cacctttccg ccacccgcgc tcaaacaagt gtatggctgg cgctggaatg aagaaa
//
LOCUS       DAOQDO010001716         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84394_length_1616_cov_3.619475, whole genome shotgun sequence.
ACCESSION   DAOQDO010001716 DAOQDO010000000
VERSION     DAOQDO010001716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84394_length_1616_cov_3.619475"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKZ53_22060"
     CDS             <1..224
                     /locus_tag="PKZ53_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43185.1"
                     /translation="QPLADQQAQLAQWKQRLKTASGDQSLGLMFLEQAEYVQGVFSKS
                     NNPDDLRRSTAVLEHVLPLYASLLKIKTP"
     gene            221..364
                     /locus_tag="PKZ53_22065"
     CDS             221..364
                     /locus_tag="PKZ53_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43186.1"
                     /translation="MKIFQGLMLILGLLWFGTTAIAHPPQTKTKKAKSAVLTVTLVRW
                     PYT"
     gene            386..1108
                     /locus_tag="PKZ53_22070"
     CDS             386..1108
                     /locus_tag="PKZ53_22070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ43187.1"
                     /translation="MLVKDVVNRYPGKAEFVSLDWGNSELPKRYGITKYPVVFVGDVL
                     VAKPEDFGGWGVTESKYGPISEKANQERFKQDLARMIDILLANPNQKPQVKKVGATSK
                     ELTVLPDFDWKDIDGQPLKSSELAGKITIVEFWATWCPPCRSTLSWLGELKAEHPDQL
                     NIVGIAIDSEEAKIKEMTEKLRPSVHIVNGSKDRISKFGIITSVPTLYVFDQNGRLVQ
                     AFYGAPPDLHAKIKQVLSQIAK"
     gene            complement(1308..>1616)
                     /locus_tag="PKZ53_22075"
     CDS             complement(1308..>1616)
                     /locus_tag="PKZ53_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43188.1"
                     /translation="TKAEKSGYARGAADFERTYTEYFRRFRALECEVVIEFDGIAGEI
                     SGGKFEDHDVTLAGLSFRRKLEHERKGTRMDLKDVPPVLLCEAVADYEAIGAMGKKRA
                     "
BASE COUNT          410 a          437 c          396 g          373 t
ORIGIN      
        1 cgcagccgct ggccgaccag caagcccagc ttgcccagtg gaagcaacgg ctcaaaactg
       61 cctctggcga tcagagtctt ggattgatgt ttctcgaaca ggccgaatat gtgcagggtg
      121 ttttttccaa atccaacaac cctgacgatc tccgacggag cacggcggtg ctggagcatg
      181 tgttgccgct ctatgcctcg ctgttgaaaa taaaaacacc atgaaaattt ttcagggatt
      241 gatgctcatc ctgggcctgc tctggtttgg tacaaccgcc atcgcccacc caccacaaac
      301 aaagacgaaa aaagcaaagt ccgctgtttt gaccgtgacg ctggtccgct ggccatacac
      361 ctgagggctc tctgacccgg caagtttgct ggtcaaagac gtcgtcaatc gctatcccgg
      421 aaaagccgaa ttcgtcagcc ttgattgggg caattccgaa cttcccaaac gctatggtat
      481 caccaaatat ccagtggtgt ttgttggtga tgtgctggtc gccaaacccg aagattttgg
      541 cggatggggt gttaccgagt ccaaatacgg gcccatcagc gagaaggcca accaggagcg
      601 atttaaacag gatctggccc gaatgattga tattctgctg gccaatccca atcagaaacc
      661 gcaggtcaaa aaagtcggcg ccacctcaaa agaactgacg gtgctgcctg attttgactg
      721 gaaagacata gacggccagc cactcaaatc aagcgaactg gccgggaaaa tcacgattgt
      781 tgaattttgg gcgacgtggt gcccgccctg tcgctcgaca ctaagctggc ttggggaact
      841 caaagccgag caccctgacc agctcaacat tgtcggaatt gcaattgatt ctgaagaagc
      901 caaaatcaag gaaatgaccg aaaaactccg gccttcggtt catatcgtca acggctcaaa
      961 agatcggatt tcaaaattcg gcatcatcac cagcgttccg acgctctatg tgtttgatca
     1021 aaatggaagg ctggtgcagg cgttctatgg cgcaccgccg gacctccacg ccaagatcaa
     1081 acaggtgctt tcccaaatag caaaatagcg agtcgcgagt cgtgggtagt caatatgaaa
     1141 gagtatggtg aggagagcag ttcctggatg tagcatcggc acgttctagg accacttaaa
     1201 gcattctttt acagtatttt aacctgttgt gaacgcgttc acaatttgtt ccaatgctga
     1261 ccgatgcttc aatcaccttg tgagtcttac tactcacgac tcgtttttta tgcccgtttc
     1321 ttccccattg ccccaatggc ctcataatcg gcaacggctt cacacagcag cactggcggc
     1381 acatccttga ggtccatccg ggtgcccttg cgttcgtgtt cgagcttgcg gcggaagctc
     1441 aacccggcca gcgtgacgtc gtggtcttca aactttccac ctgagatttc accagcgatg
     1501 ccgtcaaact caatcacaac ttcacattca agcgcccgaa agcggcggaa atattcagta
     1561 taagtccgtt caaaatcagc cgcaccgcga gcatagcctg acttttcggc tttggt
//
LOCUS       DAOQDO010001717         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84399_length_1616_cov_3.274824, whole genome shotgun sequence.
ACCESSION   DAOQDO010001717 DAOQDO010000000
VERSION     DAOQDO010001717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84399_length_1616_cov_3.274824"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKZ53_22080"
     CDS             <1..1049
                     /locus_tag="PKZ53_22080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43189.1"
                     /translation="MTGPESQSTSPAGYKLLDTYARNLPHVESFSICTMMQLVNGFPN
                     GDKVQAFLKRTDGNYWRIFDFQFLEGGPFLEEDNQKANFVAVINDATRQKFFGNTTAI
                     GKTIELDGQRFRVVGVVTNVPITRLAPFSDVWVPLNTAKSDSYRNELMGGHTGVILAE
                     NAAAIPLIKAEFDTRVQSVEIPDPKRYTEFLSRAETLWEFVARTLTGDASSRATMKLL
                     VGALSFMFLFMLLPAINLINITVSRILERSSEIGVRKAFGASSMTLIGQFVVENVILT
                     LVGGVLGLVGSWLVLRGISESGLIPYAQYQINWRVFLYGLGLALVFGVFSGVYPAWKM
                     SRLHPVEALKGGVR"
     gene            1046..>1616
                     /locus_tag="PKZ53_22085"
     CDS             1046..>1616
                     /locus_tag="PKZ53_22085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43190.1"
                     /translation="MIKQLFKLTWNRKRANLLISIEIFISFLVLFAVVLMAGVYIYNY
                     LQPLGFEYDNVVCVSVDTSARSHDAKSNAAERELFKQLQLSAQSLGEVESVALSNVDP
                     FGNSQNVSGFERNGREIQYSLSRVTDRYKDVMRLQLVAGRWFSREDDGSKFQPVVISQ
                     RLAREGFGTENPIGKAIEKLVELARAEVPF"
BASE COUNT          366 a          375 c          448 g          427 t
ORIGIN      
        1 agatgacggg cccggaatct caatccacca gccctgccgg gtacaaactc ctcgatacat
       61 atgcccgaaa cctgccacac gtcgaatcct tttcgatttg caccatgatg caactggtta
      121 atggatttcc aaatggcgat aaagtgcagg cgtttctcaa gcggacggat ggaaactatt
      181 ggcgaatctt tgattttcag tttctcgaag gcggaccgtt tttggaagaa gacaaccaga
      241 aggcaaattt tgtagccgtg atcaatgacg ccacccggca aaagtttttt ggtaatacca
      301 ccgcgattgg caaaacgata gaactcgacg gtcagcgatt ccgcgtggtc ggagtcgtta
      361 ccaatgtgcc aattacccgc ctcgctcctt tttctgatgt ctgggtgccg ctgaacacag
      421 ccaaatctga tagctatcga aatgaactga tgggtggtca caccggagtg attctggccg
      481 aaaatgctgc tgccattcct ctgataaaag ccgaatttga tacccgggtt caatcggttg
      541 agatcccaga cccgaaacga tataccgaat ttctcagccg ggcggaaaca ttgtgggaat
      601 ttgttgcccg gaccttgacg ggggacgctt cctcacgcgc caccatgaaa ctcctggtgg
      661 gcgccttgag ctttatgttt ctgtttatgt tgttgccggc cattaactta atcaacatca
      721 ctgtcagtcg catcttagag cgctcaagtg agatcggagt ccgcaaagcg tttggtgctt
      781 catctatgac actcatcggc cagtttgtcg tggaaaacgt gatcctgacg ctggtgggcg
      841 gagtactggg gctggttggc tcgtggctgg tattgcgtgg tatctcggaa agcgggctga
      901 tcccctatgc ccagtatcaa atcaactggc gggtttttct gtatgggctg gggctggcgc
      961 tggtcttcgg ggtgttttcc ggggtgtatc cagcctggaa aatgtcacgt ctccatccgg
     1021 ttgaagccct caagggaggt gtccgatgat caagcagctt tttaaactca cctggaatcg
     1081 gaagcgggcc aacctgctga tttcaattga aatatttatt tctttcctgg tgttgtttgc
     1141 cgtggtgctg atggccgggg tttatatcta taattacctg cagccattgg ggtttgagta
     1201 tgacaatgtg gtgtgcgtat cagttgatac ctcagctcgt tcccatgatg cgaaaagtaa
     1261 tgccgccgag cgcgaactct tcaagcagct tcagctatct gctcagtcac tgggtgaagt
     1321 cgaaagcgtg gcgctgtcca atgttgatcc gtttggcaat agtcagaatg tctcgggctt
     1381 tgaacgcaac gggagggaaa tccagtacag tctgagtcgg gttacagata gatataagga
     1441 tgtgatgcgg ttgcaactcg ttgctgggcg ctggtttagc cgtgaagacg atggatcgaa
     1501 atttcagccg gtcgttatca gccagcgtct ggcgcgtgag ggatttggca ctgaaaatcc
     1561 aattgggaaa gcgattgaga aactggttga gcttgcacgt gcagaagtgc ctttcg
//
LOCUS       DAOQDO010001718         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84400_length_1616_cov_3.172966, whole genome shotgun sequence.
ACCESSION   DAOQDO010001718 DAOQDO010000000
VERSION     DAOQDO010001718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84400_length_1616_cov_3.172966"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="PKZ53_22090"
     CDS             complement(<1..1596)
                     /locus_tag="PKZ53_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ43191.1"
                     /translation="MNYFVTGGTGFIGKFLVERLARRKDATIYVLVRESSQDKFEFLQ
                     ERIGENGKKLVPIFGDITKPGVISPASLKKLKGKIDHVFHLAAIYDMELDEETAEAFN
                     VEGTRNVVKLVNKLGGEVRLHHTSSVAIAGSDFAGQFTEEMFDEGQTLTHPYHRTKFE
                     SEQIVREECNVPFRIYRPGVVIGSSVTGEIDKIDGPYYFFKTLQKISYKIPKWLPLLG
                     IHGGKVPIVPVDYVANAMDAIAHKPNLDGKAFFLLQANSPSVGELIETILAAAHGPGF
                     AHKLELPWLNAPLRTATESIDRVMPDRIKKQIAKAIGAPVSTLSFSFVTTVFSDRNTR
                     AALKGTGIRCPNLVDYAPVIWQFWEQYLDYDYTVPVKLVRKLTGKVVLITGASSGIGF
                     STAKKLAATGAKVLLVARTREKLEETQRIIENAGGEAYVYPCDLNDLEAIDKMAADVL
                     SDFGAVDVLINNAGRSIRRAVDESYDRFHDFQRTMQLNYFGAVRLIMALLPSMQQHRS
                     GQIINISSIGVQLNAARFSAYVAS"
BASE COUNT          394 a          447 c          388 g          387 t
ORIGIN      
        1 gctggccaca taggctgaaa accgggcggc gttgagctgg acgccaattg aactaatatt
       61 gatgatctgg ccgcttcggt gctgctgcat acttggaagc aaggccataa tcaaccgaac
      121 cgcgccaaag tagttaagct gcatcgtgcg ttgaaagtcg tgaaagcgat cataggattc
      181 atccacggcc cgtcggatcg agcgcccggc attattgatc aggacatcca cggcgccaaa
      241 gtcactcagc acatccgccg ccattttatc aatagcctct aaatcattga gatcacaggg
      301 gtaaacatac gcttcgccac cggcattctc aataatacgt tgggtttctt cgagcttttc
      361 ccgcgtgcgg gccaccagca acactttggc cccggtcgcc gccagttttt tggcagtgga
      421 aaaaccaatc ccacttgagg ccccagtaat aagcaccact ttcccggtca gtttgcggac
      481 cagtttcacc ggaacagtgt aatcataatc aagatattgc tcccaaaact gccagatgac
      541 cggggcataa tcaaccagat ttggacagcg gatgccagtc cccttgagcg ccgcccgggt
      601 gttccggtca ctaaaaaccg tggtcacaaa cgaaaaactc agggttgaaa ccggtgcgcc
      661 gatggctttg gctatttgtt tcttgatccg gtcaggcatc acccgatcaa tggactcggt
      721 cgcggttcgc aacggagcat tcaaccaggg caattcaagt ttgtgggcaa accctggacc
      781 gtgggccgcc gccagaatcg tttcaatcaa ttcacccact gacggtgaat tggcttgcaa
      841 caggaaaaag gctttcccat ccagatttgg cttatgggca atcgcatcca tcgcattggc
      901 gacataatca accgggacaa tcgggacttt tccgccgtga atcccaagca gtggcaacca
      961 tttgggaatc ttgtagctga ttttttggag cgtcttgaag aaatagtacg gcccatcaat
     1021 tttatcaatc tcgcccgtca cactcgaccc aatgaccacg ccgggccggt agatgcgaaa
     1081 ggggacgttg cattcttcgc gcacaatctg ttcagattca aacttggtcc ggtggtacgg
     1141 atgcgtcaac gtctgtcctt cgtcgaacat ctcttccgta aattgcccgg caaagtcaga
     1201 gccggcaatc gccaccgaac tggtatggtg caggcgaacc tctccaccca gtttattgac
     1261 cagcttcacc acgttgcgtg ttccttcgac attaaaggct tcggcagttt cttcatctaa
     1321 ctccatgtca taaatggctg ccagatggaa cacgtgatca atctttccct tgagcttttt
     1381 cagacttgcc ggagaaatca cgccaggttt ggtgatatcg ccgaaaattg gaaccagttt
     1441 tttgccattt tcgccaatgc gttcctgcag aaattcaaac ttatcctgcg acgactcgcg
     1501 caccagtaca taaatggttg catccttgcg ccgtgccagg cgttcgacca gaaatttacc
     1561 gatgaacccc gtgccgccgg tgacgaagta gttcatatga tttgatccat ccagag
//
LOCUS       DAOQDO010001719         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84493_length_1615_cov_3.530128, whole genome shotgun sequence.
ACCESSION   DAOQDO010001719 DAOQDO010000000
VERSION     DAOQDO010001719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84493_length_1615_cov_3.530128"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKZ53_22095"
     CDS             <1..355
                     /locus_tag="PKZ53_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNJ43192.1"
                     /translation="VGVILVAIIGNAAEHSTAVLMALKNKMDLAMNIAVGSSLQIALF
                     VAPMLVFLSYPFGGKGPMDLLFTTLEVVSIVIAVTTTSLVSQDGESNWMEGVQMLAVY
                     LILGIAFYNLPHLPL"
     gene            496..1455
                     /locus_tag="PKZ53_22100"
     CDS             496..1455
                     /locus_tag="PKZ53_22100"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HNJ43193.1"
                     /translation="MNTLSTAQTIRDGALLYFPLEVGNQWIYRSTTLEGIVIEVVETR
                     EVQGKTYFVVRGLVRGRDVLVRESKKGTLVIFDEESKTENLWYDFKTKKDDDFISPPH
                     VCAPKAVMTSRTYTYRGLIGTYSNALQLTYPGVFQCGNAQEIFVPTIGLVQWTEVRGP
                     AQYVYDLMYVRLGKNTVLTQPEIGFSITLDQSSYLTRPPGSSAGYIPHLTAKITLRST
                     HPTPLKLTFPTGQLFDFSIRDEKGEVFYTWSDGKGFPEVITEESVSGERHFVIGVPLI
                     TSDARVLPAGRYIAEAWITTQGARKFVASVGFEVVDNKAKLAN"
BASE COUNT          397 a          391 c          395 g          432 t
ORIGIN      
        1 cgtcggtgtg attttggtgg cgatcatcgg gaacgcggca gagcattcaa ccgccgtgct
       61 gatggcactc aaaaataaaa tggacctggc gatgaacatt gcggttgggt cttcactcca
      121 gatcgcgtta tttgtggcgc cgatgctggt tttcctcagc tatccatttg gcggtaaagg
      181 gccaatggat ttgcttttta ccaccctcga agtggtttca attgtgattg ccgtcaccac
      241 aacctcactg gtttcacaag atggcgagtc caactggatg gaaggcgtgc aaatgctggc
      301 cgtgtatctg atactcggaa ttgcttttta caaccttccg catctaccgt tatgacagat
      361 tgactcgtca ggttattttc aaacttgcag tgaaaggcca aagatatgcg aagtttgggt
      421 cttgtcccgg tagccgtctt gatgctggca tggattggag gcttacagtc cccaatccat
      481 gcaaaaccaa tgaatatgaa cactttgagc accgcccaaa ccattcggga tggtgctctt
      541 ttgtattttc cgcttgaagt tggcaaccag tggatctatc gcagcaccac ccttgaaggc
      601 attgtgatcg aagttgttga aacccgtgag gtccagggaa aaacctattt tgtggtgagg
      661 ggacttgtcc gagggcgtga tgtgctggtt cgtgaatcca aaaagggaac cctggtgatt
      721 tttgacgaag aatcgaaaac cgaaaacctg tggtatgact tcaagacaaa aaaggacgat
      781 gattttatat cgccgcctca cgtctgtgcc ccgaaagcag tcatgacatc cagaacatac
      841 acctatcgag ggttgattgg cacctattcc aatgcactgc aactgactta ccctggtgtt
      901 tttcaatgcg ggaatgctca ggaaatcttt gttccgacga ttgggctggt tcagtggacc
      961 gaagtccgtg gcccggccca gtatgtttac gatttgatgt atgtacggct tggaaaaaat
     1021 accgtattaa cccagccgga gatcggtttt tcaatcacgc tcgatcaatc aagctatttg
     1081 acacggcctc ccggctcgtc cgccgggtat attccacatt tgacagccaa aatcaccctt
     1141 cgaagcaccc atccaacacc attgaaactg acctttccca ccgggcaact gtttgatttc
     1201 tcgattcggg atgaaaaagg cgaagtgttt tacacctggt cggatgggaa ggggtttcct
     1261 gaagtcatca ccgaagaatc cgtttccggt gagcggcatt ttgtcatcgg tgtgccgtta
     1321 atcacatcag atgccagggt tttgcccgca ggccgctaca ttgccgaagc ctggatcaca
     1381 acgcaggggg cgcgcaaatt tgtagcatcg gttggatttg aagtggtgga taacaaagct
     1441 aaattggcca actgaaagag ccctctccac tatagcttcc agacctgagc aacccagtcg
     1501 gaatcaaatt ccttttacaa aaaattcacc aaaggcgctg tggttgtctg ctatccttag
     1561 ggtctctttg ttcgcattct ccaaacacgt ttttctcaaa cccattcatc atttt
//
LOCUS       DAOQDO010001720         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84586_length_1614_cov_3.460552, whole genome shotgun sequence.
ACCESSION   DAOQDO010001720 DAOQDO010000000
VERSION     DAOQDO010001720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84586_length_1614_cov_3.460552"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1434
                     /locus_tag="PKZ53_22105"
     CDS             67..1434
                     /locus_tag="PKZ53_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43194.1"
                     /translation="MSSPLIAQTKAHLSKRRRKPPMAFDQRILWLGFLTGLPGALVAL
                     GLLWLGDYTPKVQWTLTFLIGTSWTWLTFALQERVIRPLQTISNLLAALREGDYSIRG
                     RSDRRDDALGEVMLEINELSKTLQDQRLGTIEATAMLRTISAEIDVAIFAFDSDQILR
                     LVNRAGERLLAQPLERLLGRNASDLGLVDCLEGEPAQTVQMAFPGGIGRYGVHRSTFR
                     EHGLPHHLLVFTDLSRQLREEERQAWQRLIRVIGHELNNSLTPIKSIAGSLETLLNRQ
                     PRPPDWEDDMQSGLSVIASRAESLSRFMAAYARLARLPQPRLAPLNVPSWIQRVVALE
                     TRLSVVVVPGPMITIQADQDQLEQLLINILRNGVDASMETGGKVAVGWTRHAAYLEIW
                     VEDNGPGLSNVNNLFVPFFTTKPGGTGIGLVLSRQIAEAHGGSLILENRVPGPGCLAR
                     LHLPL"
BASE COUNT          355 a          439 c          448 g          372 t
ORIGIN      
        1 tcatccctca tcccttccga aaaccctgaa ccctgaaccc tgaaccctga accctaagcc
       61 ccaaccatgt cttcaccact tatcgcccaa accaaagctc acctgagcaa gcgtcgtcga
      121 aagcccccca tggcttttga tcaacgcata ttgtggcttg ggtttttgac tggtctgcct
      181 ggagccctgg tggcacttgg gctgttgtgg ttgggtgact atacgccaaa agtccagtgg
      241 acactgacat ttctgattgg taccagttgg acctggttga catttgcgtt gcaagagcgt
      301 gttattcgac cgctgcagac aatttccaat ctgctggcgg cattgcgcga gggcgattat
      361 tcgatccgtg gccggagcga ccgccgcgat gatgccctgg gcgaagtgat gcttgaaatc
      421 aatgaactga gtaaaacgtt gcaggatcag cgcttaggga caattgaagc cacggcgatg
      481 ctgcgtacca tctcggcgga aattgatgtt gccatctttg cctttgattc agaccagatt
      541 ctccggctgg taaatcgagc cggagaacgg ttgctggcac agccgttaga gcggttgctg
      601 ggacgaaatg cctctgattt gggacttgtg gattgcctgg aaggcgaacc ggcccaaact
      661 gtccaaatgg cttttccagg tggcattgga cggtatggcg ttcatcgcag cactttccgt
      721 gaacacgggt taccccatca cttgctggtg tttactgatc tcagccggca actccgcgaa
      781 gaagagcggc aggcctggca gcggttgatt cgcgtcattg gtcatgaatt gaacaactcg
      841 ctgacaccca tcaaatctat tgccgggagc ctcgaaaccc tgctcaaccg acaaccgcgc
      901 ccgcccgatt gggaagacga tatgcaaagc gggttgtcag tgattgcctc gcgggctgaa
      961 tcgttgagcc gctttatggc cgcctatgcc cggctggctc gattgcctca accacggctg
     1021 gcacccctca atgtgccatc gtggatccag cgggttgtgg cgcttgagac ccgactttca
     1081 gtggttgtcg tgccgggccc gatgatcacc attcaggccg atcaggatca gttggaacag
     1141 ttactgatca acatcttgcg aaatggagtg gatgcctcga tggaaaccgg tgggaaagtt
     1201 gccgttggat ggacccgaca cgctgcctac cttgaaattt gggttgagga taatggccca
     1261 ggtctttcga atgtcaacaa cctgttcgtt ccgttcttca caaccaaacc tggcggaacc
     1321 gggatcggtc tggtgctcag tcgccagatt gccgaagccc atggcggcag tttgatcctt
     1381 gaaaaccggg ttcctggccc tggctgtctg gcacggctcc acctgccgct gtaaggctga
     1441 aaaatcatcg ggctgaagaa gatgggctga aaaatcatcg ggctgaagaa ggtgggctga
     1501 aaaatcatcg ggctgaagaa ggtgggctga agaaattggg taattcagcc ctcagcccaa
     1561 agtcttcagc cctcagccca aagtcttcag ccctcagccc aaagtcttca gccc
//
LOCUS       DAOQDO010001721         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84595_length_1614_cov_3.203335, whole genome shotgun sequence.
ACCESSION   DAOQDO010001721 DAOQDO010000000
VERSION     DAOQDO010001721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84595_length_1614_cov_3.203335"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1344)
                     /gene="hpnI"
                     /locus_tag="PKZ53_22110"
     CDS             complement(187..1344)
                     /gene="hpnI"
                     /locus_tag="PKZ53_22110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HNJ43195.1"
                     /translation="MVQSVFYLVLGLVVASAIYQLVSLYSAFSFFRRHRRQTVHPDFF
                     PPVSILKPVRGMDDRALECWTSFCQQNYPGPMELVFGVRDEADPAVPFIRQLQQDFPL
                     LQITLIINPSQIGVSGKVSNLNNMLAGVHSELLVLSDSDIRVGTEYLQTVVSPFANPR
                     VGLVTCMYRAIGSTRLAALIEGIGITAEFLPGALVSERFEGIKFAMGSTIALRRTVLD
                     QIGGFPAIADYLADDYLLGKLTADAGHEVQLSPYVVETVLPDYGFREFFDHQLRWARC
                     VKYSRPGGYLGLLFTHTTVLALTLFLLSGSSVLGIGVGVGALILRLVAAWMIGVQWLQ
                     DRRLGRYFFLVPVRDILSFVVWGVSFWGTTVLWRGDRFRLEAGGKLNRIQN"
     gene            complement(1420..>1614)
                     /locus_tag="PKZ53_22115"
     CDS             complement(1420..>1614)
                     /locus_tag="PKZ53_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43196.1"
                     /translation="RWAACFEAHPPEYRYGMVRARMGAGSKLKGFAELRIPDLQDFPA
                     PIRARTSPSTGPVVARPKRR"
BASE COUNT          440 a          446 c          388 g          340 t
ORIGIN      
        1 gacattttga accacgaaac acacgaaata cacgaaaaaa accaaacact tccaaaattc
       61 aatcgctcag gaaacctttg acaagggact tatgagcctg atcagaatcc actgaagaat
      121 ttggatttat tttctgtgtg ttccgtgtat tccgtggttt cttaatctga aattcaacct
      181 tgtgggtcaa ttttgtatcc gattcagttt tccaccagct tccaatcgga aacggtctcc
      241 gcgccacagc acggttgttc cccaaaaact cactccccac accacaaaac tcagaatgtc
      301 ccgcaccggc accaggaaaa aataccggcc taagcgacga tcctggagcc actgcacgcc
      361 aatcatccag gccgcgacca gtctgaggat gagtgctccg acgccaactc cgattcctaa
      421 aactgaactt ccactcagca ggaacagtgt gagcgccagc acggttgtat gagtgaacaa
      481 caacccaagg tatccgcctg gacgggaata tttgacgcac cgtgcccagc gcaattgatg
      541 atcaaaaaac tctcgaaatc catagtcagg taaaacggtc tccacgacat agggtgaaag
      601 ctgcacttca tgtccagcat ctgcggtcag tttcccaagc agataatcat ccgccagata
      661 atcggcaata gctggaaaac caccaatttg atccaaaacg gttcgacgca gggcaatggt
      721 ggatcccatg gcaaatttga tcccttcaaa gcgttcagac accagtgcgc caggcagaaa
      781 ctcggcggta atgccaatgc cttcaatcaa ggctgccagc cgggtgcttc caattgcccg
      841 gtacatacag gtcaccagcc caactcgtgg attggcaaac ggtgagacga ccgtttgtaa
      901 atattcggtt ccaacacgga tgtcactatc gctcaatacc agcagctcag agtggacacc
      961 cgccagcata ttattgagat tgctgacctt accgctgacg ccgatttggc ttggattgat
     1021 aatgagcgta atttgaagca agggaaaatc ttgttgtagc tggcggatga atggcaccgc
     1081 cgggtcagct tcgtcccgca cgccgaacac aagctccatc ggccctggat agttctgctg
     1141 gcaaaaagat gtccagcact ccagggcccg gtcgtccatc ccacgcactg gcttgagaat
     1201 tgaaactggt ggaaaaaaat ctggatgaac cgtctgccgc cgatgccgcc gaaaaaaact
     1261 gaaggcactg tacaggctta ccagttgata gatggctgag gcgaccacaa gacccaaaac
     1321 caggtaaaaa acagattgaa ccatggaaaa actccattgc ggaaaggtca cttgatctgg
     1381 acatttcccg tccggaggac gatggagagt agccggtggt cagcgtcgct ttggacgcgc
     1441 aaccaccggg cccgtgctcg gcgaggttcg cgcccggatg ggcgctggaa aatcttgtag
     1501 gtcaggtatt ctcaattctg cgaacccttt gagttttgat ccagcgccca tccgggcgcg
     1561 aaccattcca tacctgtatt ccggtgggtg cgcctcaaag caggctgccc accg
//
LOCUS       DAOQDO010001722         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84630_length_1614_cov_2.512508, whole genome shotgun sequence.
ACCESSION   DAOQDO010001722 DAOQDO010000000
VERSION     DAOQDO010001722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84630_length_1614_cov_2.512508"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1614)
                     /locus_tag="PKZ53_22120"
     CDS             complement(<1..>1614)
                     /locus_tag="PKZ53_22120"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43197.1"
                     /translation="QYRHDRKDPTTIGDDNIRSIFQDRAGNVWVGLSGGGLNRLDYRA
                     RRFQHFTHNPDLPNTLSNESVFALHKDHHGLIWVSTSDGLNRIDPATGTVKVYPIDET
                     GQTGFPAKIATCFFEDRNDWLWIGSPRGLCRYERETDTFTCFRNDPQNPQSLSSSTVM
                     RMAQDQTGKLWLSSSGYTLNRFDPQTGTAQVFRPFPEEASEVGNNRIWSLVFGQHNIL
                     WLATSAGLFRYDPASQHIRQYVHNPKDSKSITSNRVFCLVEDADGILWVGTNGGLNRF
                     SPTTEEFSPVTTQDGLPDDNIYGIVQDDDGNLWLSTNTGLAKYNPRTRAIRAYDIRDG
                     LQSNEFNSGAYAKSPQGELLFGGINGFNLFHPRDITDNPYIPPVVITQFRQYDHLVEG
                     FNGKDIAPLRYDENYLSFEFAALNFHLPDKNQYAYQLEGFDRNWIYCGHRRFASYTNL
                     DPGEYTFRVKASNNDGVWNEAGTALRVVILPPPWRTWWAYLGYTGLLTGLVIGAFRFQ
                     QHRLQTRAQLREAELTARAIDVERSGDKTL"
BASE COUNT          376 a          410 c          448 g          380 t
ORIGIN      
        1 aagggttttg tcgccactgc gctcgacatc tatggcgcgg gcggtgagtt cggcttcacg
       61 cagttgcgca cgggtctgga gtcggtgctg ttgaaaccga aacgcaccga tcaccagccc
      121 ggtcaacagg ccggtatatc ccaaataggc ccaccaggtc cgccagggcg gcggcaaaat
      181 cacgacccgc aaggccgttc cggcttcgtt ccacacaccg tcattgtttg aagctttgac
      241 ccgaaaggtg tattcgccgg ggtcaagatt ggtataactg gcaaaacgcc gatgcccaca
      301 ataaatccag ttgcggtcaa acccttcaag ctggtaggca tactggtttt tatccggcag
      361 gtgaaaattc aaggcggcaa attcaaacga caaatagttt tcgtcgtagc ggagcggcgc
      421 aatgtccttg ccgttaaatc cttcgaccag atgatcatac tgtcgaaact gggtgatgac
      481 gaccggcgga atgtacgggt tatcggtgat atcacgtggg tgaaacaggt taaatccatt
      541 gattccacca aataaaagct cgccttgtgg tgatttggcg taggcgccgg aattaaattc
      601 gttactttgc aagccatccc ggatgtcata agccctgatc gctcgggtgc gaggattgta
      661 tttggccagc ccggtgttgg tgcttaacca gaggttgccg tcatcatcct gcacaatgcc
      721 ataaatgttg tcatcgggca ggccatcctg ggtggtaacg ggcgaaaact cctcggttgt
      781 cggtgaaaac cggttcaatc cgccattggt gccaacccag agaatcccgt cagcgtcttc
      841 gaccagacaa aatacccgat tgctggtgat acttttggag tctttggggt tgtgaacata
      901 ttgccgaata tgttgtgagg ccgggtcata tcggaataac ccggctgaag tggccagcca
      961 caggatattg tgttgaccaa acacaagcga ccagatccgg ttattgccaa cttcggaggc
     1021 ttcttcgggg aatggacgaa aaacctgggc ggtgccggtc tgtgggtcaa agcggtttaa
     1081 ggtgtacccg gaagacgaaa gccacagctt cccagtctgg tcctgggcca tccgcatcac
     1141 agtgctggag cttaaactct gagggttttg cgggtcattt cggaaacagg taaacgtgtc
     1201 ggtctcgcgt tcatagcggc agagtcccct gggtgaccca atccacagcc agtcgttccg
     1261 gtcttcaaaa aaacaggttg cgattttcgc tggaaatccg gtctgaccag tttcatcaat
     1321 cggatacacc ttgaccgtgc cggtggccgg gtcaatccgg ttgagcccat cagaagtact
     1381 cacccagatc aatccgtgat ggtctttatg taaggcaaag accgattcat tgctcagcgt
     1441 atttggcagg tctggattgt gagtgaagtg ttgaaatcgt cgggcacggt aatcaagccg
     1501 attgagcccg ccgccgctca agcccaccca gacattgcca gctcgatctt gaaaaatgga
     1561 ccgaatattg tcatcgccaa tcgtggttgg gtctttgcgg tcgtgccgat actg
//
LOCUS       DAOQDO010001723         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84920_length_1611_cov_2.748715, whole genome shotgun sequence.
ACCESSION   DAOQDO010001723 DAOQDO010000000
VERSION     DAOQDO010001723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84920_length_1611_cov_2.748715"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>1611)
                     /locus_tag="PKZ53_22125"
     CDS             complement(78..>1611)
                     /locus_tag="PKZ53_22125"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNJ43198.1"
                     /translation="AQSLQPEVFSPESFMKPLFFALLFLLLTGLVAPILAGPGYVVAI
                     GGGSEDDRTPTSWSGVVYQQIVERGNRGKVVILGTEPESDFLPRYFRSLGASDAVNLT
                     VSSSKTANKKRTLKALETASVVFIRGGDQAEYIRLWKGTRLATAIRDLYQRGGVVAGT
                     SAGAMILGDVVYTAENGTIFPEEALRNPFNQYLTVSEGFLNLIPGVMFETHYTERGRI
                     GRLGPMLARWWTANPTSPAVGIALDDQTAVMISPTLEAEVLGQGIAGVMRRTPQTQID
                     LKPGEAPLITHLQYDQLTEGFRFDVTSQTLTKTPASARPVTLLPPPTVFTPTILNGTE
                     AAAASFGEHTVTGVTDDPFGLYLGKLNVTAGSGQVPQTLVMPRVYSEVKFIENRTGGL
                     FFGLATAQVRTGILMDQGSTASLESDGTLNFDAKNSGHPSLLVISLAGVSLVDFSTYS
                     TDRDSVGPRQSAALVGMLVHYLPSGHSYHLGSGEVGTLERKGQGNGQGYEKTKRTEKT
                     QH"
BASE COUNT          368 a          449 c          414 g          380 t
ORIGIN      
        1 gggccagaat ccgagtgcga agtgtatgtt ccatagtttt tgagggttcc gggttccggg
       61 ttccgggttc cgggttttca atgttgggtc ttttctgtcc tttttgtctt ttcgtacccc
      121 tgcccgttgc cctgcccctt acgttccaaa gtgccgactt ctccgcttcc taaatgatag
      181 ctgtgtcctg acggcagata atgaaccagc atcccgacga gggcggctga ttgacgtggg
      241 cctacactat cacgatcagt actataagtc gaaaaatcaa ccagactgac acctgccagt
      301 gaaatgacca gcaacgacgg atggcctgag tttttggcat caaagttgag cgtaccatct
      361 gactccaggc ttgccgttga accctggtcc atgaggatcc cggtccggac ctgagcggtg
      421 gccaatccga aaaagaggcc gccagttcga ttttcaataa atttgacttc cgaataaacc
      481 cgtggcatca ccagcgtttg cggcacctga ccagatccgg cagtcacgtt taattttcca
      541 aggtagagtc caaaggggtc gtctgtcacg ccagtcacgg tatgctcacc gaaactggcc
      601 gctgccgctt cggttccatt caaaattgtc ggtgtaaaaa cagtcggcgg aggcaaaagc
      661 gttaccggac gagcactggc tggcgttttg gtcagggtct ggctcgttac atcaaagcga
      721 aacccttcgg tcaactggtc atactgaagg tgggtaatca acggcgcttc gccaggcttc
      781 aaatcaatct gggtttgggg cgtgcgccgc atcacaccgg caattccctg ccccaggact
      841 tcggcttcaa gcgtgggcga aatcatcacc gctgtctggt catcaagggc aattccgacc
      901 gctggtgatg ttggattggc cgtccaccag cgagccagca tgggtcccag tcggccaatt
      961 cggccacgtt cggtgtaatg ggtttcaaac atcacacctg gaatcagatt taaaaaccct
     1021 tctgaaacag tcagatattg attgaatgga ttgcggagtg cttcttcggg aaaaatcgtc
     1081 ccgttttctg cggtataaac aacatcgcct aaaatcatag cgccggcgct ggttccagcc
     1141 acaacgccgc cacgctgata gagatcacga atcgccgtcg cgagcctggt gcccttccac
     1201 aaccgaatgt attcggcctg atcaccgccg cgaataaaca ccacgctggc agtttccagc
     1261 gcttttagtg ttcgtttttt gttggctgtc ttcgatgagg agacagtcag gttgaccgcg
     1321 tcgctcgccc caagcgaacg aaaatagcgc ggcagaaagt cgctttcggg ttcagttccc
     1381 aaaatcacga cttttcctcg attgccgcgc tcaacaatct gttggtacac cacacccgac
     1441 cagctcgtgg gtgtccggtc gtcttcactt ccacccccaa ttgccaccac ataccccggc
     1501 cccgccagaa ttggcgcaac caaaccagtc agaagaagaa agaggagtgc aaaaaagagt
     1561 ggtttcataa agctttccgg gctgaaaact tcgggctgaa gactttgggc t
//
LOCUS       DAOQDO010001724         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_84939_length_1611_cov_2.241003, whole genome shotgun sequence.
ACCESSION   DAOQDO010001724 DAOQDO010000000
VERSION     DAOQDO010001724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84939_length_1611_cov_2.241003"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKZ53_22130"
     CDS             <1..222
                     /locus_tag="PKZ53_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43199.1"
                     /translation="MKNDLNFAQKSTALLNDQCRPVRVIQGSHQSARSVRELEFQRNR
                     AFAVTNLTSNRSFRSFLSFLSFDFGLIRY"
     gene            complement(390..1448)
                     /locus_tag="PKZ53_22135"
     CDS             complement(390..1448)
                     /locus_tag="PKZ53_22135"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ43200.1"
                     /translation="MKYALIPSARTLTACVLALTFIVVPTARGQEPEVIDQTIARVNR
                     DIITRSMYKKAESRLKEELPQITPDPKKQEELLGKLQKRILASLIDDKLIAQRADELG
                     INVEPEVNQAVIELCKQNNLELAACQEAMEKQGLSMDEIKGNFRNQLRKRSLMSQEVY
                     GPIFEKLTEAEKKDYYAKHKDQFSMPGEMVLSEIYVSYTAETLKDAEARVRDAMAELR
                     AGKEFCTVVKKYSDDKRASKPKCGNLPPFKDGELADDLKREVDKIKPGQFTSILKMPN
                     AFQILKLESRVESKAKPFEDVANEISSRLAYEKSKTKIKEYFKSLREKAYIKVADGYE
                     DNFDLEDEPIAGETEGGQ"
BASE COUNT          380 a          388 c          364 g          479 t
ORIGIN      
        1 atgaagaatg acctgaattt cgcccaaaag agcaccgcgt tgctcaacga tcagtgccga
       61 cccgtccgag tcatccaggg cagtcaccag tcggcacgat cagtgaggga acttgaattt
      121 caacgaaatc gagccttcgc ggtgacaaac ctgacttcca atcggtcctt tcggtccttt
      181 ttgtcctttt tgtcctttga ttttggtctc atcaggtact aaaaccaggg aacagatcca
      241 gtattttggg caaaatttgg ggaatgaaga atgaagaaaa ggcaagtgtt tagtgctttg
      301 gtgcttagga atcaatatct tccaagaacc aagaaccagg aaccagtcac caaacaattt
      361 cttcattctt cattctcaat tcttcattct tattgtccgc cttcggtttc accagcaatt
      421 ggctcgtctt ccaggtcaaa gttatcttca tatccatcag caactttgat ataggctttt
      481 tcacgcaggg atttaaagta ctctttgatc ttggttttgc tcttttcata ggccagccgg
      541 cttgagattt cattggcaac gtcttcaaac ggtttggctt tggattcaac tctggattcc
      601 agtttgagaa tctggaaggc attcggcatt ttgagaattg acgtaaactg gcccggcttg
      661 attttgtcca cctcgcgttt caaatcatca gccagctcac catctttgaa cgggggcagg
      721 tttccacact tgggtttgga agcacgcttg tcatcagaat attttttgac cacggtgcaa
      781 aattcttttc cagcccggag ttctgccatc gcatcacgaa cccgagcctc agcatctttg
      841 agcgtctcag ccgtgtaact gacataaatc tcactcagga ccatttcccc aggcattgaa
      901 aattgatctt tatgtttggc gtaatagtct ttcttttcag cttcggtcaa tttttcaaaa
      961 atcggtccat aaacttcctg cgacatcaag gaccgtttgc gcaactgatt gcgaaagttg
     1021 cctttgattt cgtccattga caggccctgc ttttccattg cttcctggca ggcggccagt
     1081 tctaaattgt tctgtttgca cagttcgatc acggcctgat tcacttcagg ttcgacattg
     1141 atacccagtt catccgcccg ttgagcaatc aatttgtcat caatcagcga tgccagaatc
     1201 cgtttttgca acttcccaag cagttcctcc tgcttttttg gatccggggt gatttggggg
     1261 agttcttcct tcaagcggct ttccgctttc ttgtacatcg aacgggtaat gatgtcgcgg
     1321 ttaacacggg caatcgtctg gtcaatcacc tcgggctcct ggcctcgggc cgtgggaacc
     1381 acaataaaag tcagcgccag tacacaggcg gtcaatgttc gagcggatgg aatgagtgcg
     1441 tatttcataa cgtgcacagt ttaagccttt ctgacagttg aagattcagc ttcgggcgcc
     1501 ggcttgagtt tggccagcac agtgtgaatc tgggcaaaaa attcggcatt gttcgcagat
     1561 ttcacaggat atttgaaaag accggaaggt gaaaagctga cttgtggatt g
//
LOCUS       DAOQDO010001725         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85015_length_1610_cov_3.070740, whole genome shotgun sequence.
ACCESSION   DAOQDO010001725 DAOQDO010000000
VERSION     DAOQDO010001725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85015_length_1610_cov_3.070740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..676)
                     /locus_tag="PKZ53_22140"
     CDS             complement(11..676)
                     /locus_tag="PKZ53_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43201.1"
                     /translation="MKQQSIYGIALIVGSLAGIVTMIFHPSGLSHTAPPDQMGHLAQV
                     AVATHALALIGLPIVVFGFLGFTLNQGADRPLIQAGFVAYVFASFWVSISAVSSGLIA
                     PQLIRQMVGADESAQKVLQAILSYNFQLNQGFAKLFVIGSSVAVILWSISLLKSGTVR
                     WLGILGCVVGLVNLFVFGLGHLRLNVHGFGLFTFIQSTWVILLGVMMCRPIEFFPSGK
                     SSG"
     gene            824..1399
                     /locus_tag="PKZ53_22145"
     CDS             824..1399
                     /locus_tag="PKZ53_22145"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ43202.1"
                     /translation="MEKQKKDRRTRRTRDMLGDALIELILEKHYDEITVQNVIDRANV
                     GRATFYAHFRDKEDLFLSDFENLLDGVNQHIQLEQAGTAQIVPIRQLFSHLQDFHHLY
                     RALAMSRKMELMLKTGTEYLSKKIEHKLVSQSVSTSVPPAILAHHIASTLFSLLKWWL
                     ESNMPYSPERMDEIFHELVAPGIQQTFEKGI"
     gene            1396..>1610
                     /locus_tag="PKZ53_22150"
     CDS             1396..>1610
                     /locus_tag="PKZ53_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43203.1"
                     /translation="MTLQPILRPAIPEDIAWIVAQEQRSEFEAFIGHWPAETHLKNLA
                     DPDKRYLMATDEAGQPGLLRHPRLMKAG"
BASE COUNT          481 a          429 c          373 g          327 t
ORIGIN      
        1 cccgatgtct tcaacccgat gatttcccag atggaaaaaa ctcgattgga cgacacatca
       61 tcactcccaa caaaatcacc caggttgatt gaataaaggt aaacaaacca aacccatgca
      121 cattgagacg aaggtgacca aggccaaaaa caaacaggtt gaccagtcca accacacacc
      181 cgagaatccc cagccagcga acagttcctg atttcaacaa agaaattgac cagagaatca
      241 ccgccactga tgaaccgatc acaaagagct ttgcaaaccc ctgattgagc tggaagttgt
      301 aggataaaat ggcttgcagg accttttggg ctgattcatc tgccccaacc atttgccgaa
      361 tgagttgtgg tgcaatcagc ccgctggaaa cggctgaaat tgaaacccaa aacgaagcaa
      421 agacataggc cacaaaccca gcctggatca atgggcgatc tgctccctgg ttgagagtaa
      481 atcctagaaa cccaaagacc acgattggaa ggccgatcag tgccagagca tgcgtagcca
      541 ccgccacttg agccagatgc cccatttgat caggtggcgc agtgtggctc agccccgagg
      601 ggtggaaaat catggttaca atacccgcca gagaaccgac aatcagagca attccataga
      661 ttgactgctg tttcatgggt caacgtcctt ctgatattga aaaataaacg gttgaccgca
      721 agtctatcca ggagatgaaa tcaggtcacc aaacagcctg gccgggtttg ttccaaactg
      781 aacaacccgg cctggattgt tcgaaaacgt gaggagcttg atgatggaaa aacagaaaaa
      841 agatcggcgg acccgtcgca ctcgggatat gttgggagac gcgctgattg aactcatctt
      901 ggaaaagcac tatgacgaaa tcacggtgca gaatgtgatt gaccgtgcca atgtcggacg
      961 ggctacgttt tacgcccatt tccgcgacaa ggaggacctc tttctcagcg atttcgaaaa
     1021 tcttctggat ggagtcaacc agcacatcca gttggaacag gctggcacag ctcaaatcgt
     1081 cccgattcga cagctcttta gccaccttca ggactttcat cacctgtatc gagccctggc
     1141 gatgtcgcga aaaatggagt tgatgctcaa aaccgggact gagtatctga gcaaaaagat
     1201 cgaacacaag ctggtgtcac aatctgtctc aacatccgta ccgccagcga ttctcgcaca
     1261 ccatatcgcc agcaccctgt tttccctgct gaaatggtgg cttgaaagca acatgcccta
     1321 ttctccagag cgaatggacg aaatattcca cgaactggtc gcacctggaa ttcaacagac
     1381 atttgaaaaa ggaatatgac acttcaacca attctccgtc cggcaatccc ggaagatatc
     1441 gcctggatcg tcgcccagga acaacgctct gaatttgaag catttatcgg tcactggccg
     1501 gctgaaacac acttaaaaaa cctggcagac ccggataaac gctacctgat ggccactgat
     1561 gaagctggac aacccggcct acttcgccat cctcgactga tgaaggccgg
//
LOCUS       DAOQDO010001726         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85117_length_1609_cov_2.729730, whole genome shotgun sequence.
ACCESSION   DAOQDO010001726 DAOQDO010000000
VERSION     DAOQDO010001726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85117_length_1609_cov_2.729730"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..>1609)
                     /locus_tag="PKZ53_22155"
     CDS             complement(169..>1609)
                     /locus_tag="PKZ53_22155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ43204.1"
                     /translation="QVLSPEQLRAVIAHELGHLSGNHGRFSRWIYHSRQTWGRLLNQL
                     KSNQDQLTTAIFEAFLKWYGPFFNAYTFVLARTQEYEADQAAAEFAGISYAAEALIET
                     DIKATFLTEVYWPSVFQKAETEPDPVAGVYAEIPRVLYRDGIDPELVTKVLKQALGAE
                     TSYDDTHPSLRERLQAIRFLPRTADEISGWSETYQPKPFKQSAAEYFLAESLPWLLKA
                     AETEWHESVKEEWADRHAFLTKARSELTRIEAKVQTDVLTSDEILDRAFLTKILKGLE
                     SAVELFQEALNHFPDHPEANYQYGVALLEQGNAEGVRHLEKAMNQDIEAVMPGCRRIF
                     EFYQDQNQPEEAERYRGRAQQHYEEMQLAQEERQSFTLSDELVPHTATSEQVRTLLHY
                     LRGNPKIKAAYLAQKRVQHFPDYPYFVLGIVPDFPWYKLSRDSAQSELLQLVINQAPF
                     PGEAYVILLVNDTRGFQKKFEKIYNSRIF"
BASE COUNT          359 a          398 c          425 g          427 t
ORIGIN      
        1 gtatatgctc cgttgaaatg gaatcgggtt cagggtttag ggtttaggtt cagggcagaa
       61 ccatctcaag tgagcggtac ctgccgattc tccaggcctc agcttgagtg ggatcagttg
      121 cttgatcact tataccctga aaccctgaac cctgaaccct gacaagaatt agaagattct
      181 tgagttatag atcttttcaa attttttctg aaaccctcgg gtgtcgttca ccagcaaaat
      241 cacataggct tcacctggga acggcgcctg attgataacc agttgcaata attctgactg
      301 cgctgaatcg cggctcagtt tgtaccacgg aaaatcaggc acaatcccca gcacaaaata
      361 tgggtaatca ggaaaatgct ggactctctt ctgcgccaga taagcggctt tgatttttgg
      421 atttccccgc agatagtgaa ggagcgttcg aacctgctcg ctggtggccg tgtggggcac
      481 taattcatcc gacagggtaa aggattgccg ttcctcttgg gccagttgca tctcttcata
      541 gtgttgctgg gcacggccac ggtagcgttc ggcttcctcg ggctggtttt gatcctgata
      601 aaactcaaaa atccgccggc agccaggcat caccgcttcg atgtcctggt tcatggcctt
      661 ttccagatgg cggacaccct cggcatttcc ttgttccagc agagcgaccc catactgata
      721 gttggcttca gggtggtcgg gaaagtggtt gagggcttct tgaaacagtt caacggctga
      781 ttcaagcccc ttgagaattt tggtcagaaa cgctcgatcc aggatttcgt cgctggttaa
      841 cacatcggtt tgcactttgg cttcaatccg agttaattcc gatctggctt tggtcaaaaa
      901 ggcgtgacga tctgcccact cttctttgac cgattcgtgc cattcggttt cagccgcttt
      961 gaggagccac ggcagacttt cggccagaaa atactctgcc gcgctctgtt taaagggttt
     1021 tggttgatag gtttcactcc aaccggagat ttcgtctgcg gtgcgcggca aaaagcggat
     1081 cgcctgaagg cgttctctca aggatgggtg cgtgtcgtca taactggttt cagccccgag
     1141 agcctgtttc aggactttgg tgaccagttc cgggtcaatg ccgtcacgat acaggactct
     1201 gggaatttca gcatacaccc cggccaccgg gtctggctcg gtttcagctt tttgaaacac
     1261 cgatggccag tatacttcgg tcaaaaacgt cgctttgata tcggtttcaa tcagggcttc
     1321 ggcggcatag ctgattccag caaattccgc cgctgcctga tcggcttcat attcctgggt
     1381 tcgggccaga acgaacgtgt aggcattaaa aaatgggcca taccatttta agaatgcctc
     1441 gaaaatagcc gtggtgagtt gatcctgatt tgatttgagc tgattgagca atcgtcccca
     1501 ggtctggcgg ctatggtaaa tccagcgtga aaaccggcca tgattgccgg aaagatgccc
     1561 gagttcgtgg gcgataaccg cccgaagttg ttctggtgaa agaacctgg
//
LOCUS       DAOQDO010001727         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85205_length_1608_cov_3.383773, whole genome shotgun sequence.
ACCESSION   DAOQDO010001727 DAOQDO010000000
VERSION     DAOQDO010001727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85205_length_1608_cov_3.383773"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="PKZ53_22160"
     CDS             <1..1518
                     /locus_tag="PKZ53_22160"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HNJ43205.1"
                     /translation="AVDAANTAYLNSQVTARLRLVQATEVNYAETPDFFQMLEWVRTD
                     PTVAARRNACQADLVSMLVNNTSLGGRGYLMQSGNIGPAFEAYAFSVVCWYYASSYYV
                     LAHEVGHNLGCHHDVANTTEQGAYPFSYGQYYSGIYRTVMAYDNGCQCPMYAGFSNPN
                     LTFQNLPTGIVNQTDNAQTINQTAPIAANFRGPGGTIPLLTLAAPNGGETLMAGTAVQ
                     IVWTTTTDYLETAVRLELMRNGTLQQVISASTSTGSNGIGTFSWVIPTNVTPGNDYQI
                     RVSHPSNSTVQDVSDATFTLSGPNTATLTVTTPNGGESWALNTRQTVRWTYSDNPGSA
                     VKVELFKNGILRQVIAAAAPVGSNGSGSFDWDISSAELPGNDYTVKITSTSFSTISDV
                     SNNTFSLGSVPVNVTIQVVTPNGGEKWKPRTQQQILWSFTGNPGAKVKVELYKGGVFK
                     QIISAGVAIGTNGAGSLSWLVPSTLAAGSDYKIKITSTTTASATDFSNQNFSVIR"
BASE COUNT          410 a          414 c          421 g          363 t
ORIGIN      
        1 gccgtagatg ccgccaacac tgcctatctc aacagtcagg tgactgcccg gctgcggctg
       61 gttcaggcaa cggaggtgaa ctatgcggaa acacctgact ttttccagat gctggaatgg
      121 gtacgaactg accccacggt tgctgccaga cgcaatgcct gtcaggcaga cctggtttca
      181 atgctggtga ataacacgtc gctgggtggc cgaggctact tgatgcaatc cggaaacatc
      241 ggtcccgcct ttgaagccta tgcgttttcg gttgtttgct ggtactacgc atccagttac
      301 tatgtgctgg cccacgaagt cggccacaat ctcggctgtc atcacgatgt ggcaaataca
      361 actgagcagg gagcttatcc attttcctac ggtcagtatt attcaggtat ctatcggacg
      421 gtgatggcct atgacaacgg gtgccagtgc ccgatgtacg ccgggttttc aaatccaaat
      481 cttacctttc aaaacttacc gactggtatc gtcaaccaga ccgacaatgc ccaaacgatc
      541 aaccagaccg caccgattgc cgcaaatttc cgtgggccag ggggaaccat tccattgctt
      601 accctggcag cacccaatgg aggggaaact ctgatggcgg gaactgcggt tcaaattgtc
      661 tggaccacga ccactgacta cctggaaaca gcggttcgat tggaactgat gcgtaatgga
      721 actcttcagc aggtgatttc ggcttcaact tcaactggaa gcaatggaat cggcactttt
      781 tcctgggtga ttccaacgaa tgtcacacct gggaacgact accagattcg ggtgagtcac
      841 ccatcaaatt ccaccgttca agatgtaagt gacgcaacgt ttaccctcag tgggccaaac
      901 actgcgacac tcacggtgac aacacccaac ggtggggaat cgtgggcact caacacccgg
      961 caaacggtcc gctggaccta cagcgataat ccggggagcg cggttaaagt tgaattgttc
     1021 aaaaacggaa ttcttcgaca ggttatagca gcggcagcgc cagttggaag taatggaagt
     1081 ggttcatttg actgggatat ttcctctgct gaattgcctg gaaatgacta cacggtaaaa
     1141 atcaccagca cctcgttctc gaccatcagt gatgtcagta acaacacttt ctcactggga
     1201 agcgtgccgg tgaatgtcac catccaggtc gtgacgccca acggtggcga aaaatggaaa
     1261 ccccggactc agcaacaaat tctctggagt ttcacgggaa atccaggggc caaggtcaaa
     1321 gttgaactct acaaaggcgg agtgttcaaa cagatcatca gtgccggtgt ggcgattggc
     1381 accaatgggg ctgggagcct gagctggctg gttccttcaa ccctggccgc tgggagtgac
     1441 tacaaaatca aaatcaccag caccaccact gccagtgcca ctgatttcag caaccagaat
     1501 ttcagtgtga ttcggtaagt gagggagaac tggtgcttgg tgcttggtgc ttggtacaaa
     1561 tccccataaa tgagggatgg aattcggttc tttgatcgaa tcaaatcg
//
LOCUS       DAOQDO010001728         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85214_length_1608_cov_2.889891, whole genome shotgun sequence.
ACCESSION   DAOQDO010001728 DAOQDO010000000
VERSION     DAOQDO010001728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85214_length_1608_cov_2.889891"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..347
                     /locus_tag="PKZ53_22165"
     CDS             57..347
                     /locus_tag="PKZ53_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNJ43206.1"
                     /translation="MLELRPNCEHCDRDLPPNAIDAMICTFECTFCVDCVDTVLHNVC
                     PNCGGGLVRRPIRPHRLLDKYPPSQQRVFKPVKLEEHQARLEQLRDIPPAQR"
     gene            377..>1608
                     /locus_tag="PKZ53_22170"
     CDS             377..>1608
                     /locus_tag="PKZ53_22170"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ43207.1"
                     /translation="MPHSQKPPSLSLAELHEQIDQACDTLRQHNHHVLQETAKTLAAL
                     GLAAVEPLMEKLHDPDRYVQDVVARALGQLGDSRPVVLLLEIIKDYQPLSDDEKDEED
                     LSEPEFRAVEALGKIGDRRVVEPLLDELDRLVAIQPDVVTTEIIYCLGEIGDRRALET
                     CLRFLKPPLVDHSHWKAAHYALPKFGEPAVAPLIELLNEVDDPLIAYDLAEIGHPSAI
                     PVLYEVLEDTWRPDHLRANAAHALGKLKAPNAFETLLRLLNHEADNPNLLRGVILGLG
                     NTQDERALEALLKLFDHPFDQALFGWIIEALGELGTSGAIDVLVVLLDSQDMHIRQLA
                     IRALERGGDERAIPALQLLVDDYERPTSLMLDETTEAGIKQMLGPDGVDEVLDLFGEG
                     TRRWAASAIRAIRQRSQIS"
BASE COUNT          380 a          458 c          403 g          367 t
ORIGIN      
        1 attcttcatt cttcattctt cattcttcat tcttcattct tcattctcaa ttctatatgc
       61 ttgaacttcg acccaactgc gaacactgtg accgggacct tcccccaaat gcgattgacg
      121 ccatgatctg cacctttgaa tgcacgtttt gtgtggactg tgttgatacg gtgttgcaca
      181 acgtgtgtcc aaactgcggc ggcggcctgg ttcggcgacc aattcggcca caccggcttc
      241 tggacaaata cccgccctcc cagcagcggg tgttcaaacc tgtcaagctt gaagaacacc
      301 aggcacggct cgaacagctc cgggatatcc ctccggccca acgatgaatt gtatttctcc
      361 aaaaggctta atcggcatgc cacactctca aaaaccccct tcgctctctc tggccgagct
      421 tcacgaacaa attgatcaag cctgtgacac cctgcgtcaa cacaatcatc acgtactcca
      481 ggaaacggcc aaaacactgg cagcactggg tctggcagcc gtcgagccgc tgatggaaaa
      541 gctgcacgac cctgatcgct acgtacagga tgttgtggcc cgtgcgcttg ggcaacttgg
      601 tgactcacga ccagtcgttt tgctgctgga aataatcaag gactatcaac cactttcaga
      661 tgatgaaaaa gacgaagagg acctgtcaga acctgaattt cgggcggttg aagcgcttgg
      721 aaaaattgga gaccggcgcg tggtggaacc acttttggat gaacttgacc ggctggtggc
      781 catccagcct gacgtcgtca ccacggaaat tatttattgt cttggcgaaa ttggggaccg
      841 acgtgcgttg gaaacctgcc tgcgatttct caaaccacca ctggttgatc acagccactg
      901 gaaagcggct cactacgcat taccaaaatt tggtgaaccg gcagtggcac cgctgattga
      961 gctgctgaac gaagttgacg acccgctcat tgcctacgat ctggctgaaa ttggccaccc
     1021 gtcggcaatt ccggtgttgt atgaagtact tgaagatacc tggcgaccag accatctgcg
     1081 agccaatgcc gctcacgcgt tgggaaaact caaggcgccc aatgcctttg aaacactgct
     1141 caggttgctc aaccacgaag cagacaaccc caatttgctc cgcggagtca tcctgggtct
     1201 gggcaacact caagacgaac gcgccctcga agcactgctc aaactctttg atcatccctt
     1261 tgatcaggcc ctgtttggat ggatcattga agcgctgggc gagcttggaa cctccggtgc
     1321 gattgatgtg ctggtcgtgc tactcgacag tcaggacatg cacattcgac agcttgccat
     1381 ccgcgccctg gaacgtggtg gcgatgaacg agccattccg gcacttcaac ttttagtgga
     1441 tgactacgaa cgaccaacca gcctgatgct tgacgaaaca accgaagccg gaatcaagca
     1501 aatgcttggc ccggacggcg ttgatgaagt actggattta tttggcgaag gcactcgccg
     1561 ctgggctgcc agcgccatcc gggccattcg ccagcgttcg cagatttc
//
LOCUS       DAOQDO010001729         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85223_length_1608_cov_2.643915, whole genome shotgun sequence.
ACCESSION   DAOQDO010001729 DAOQDO010000000
VERSION     DAOQDO010001729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85223_length_1608_cov_2.643915"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1592)
                     /locus_tag="PKZ53_22175"
     CDS             complement(111..1592)
                     /locus_tag="PKZ53_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HNJ43208.1"
                     /translation="MQKRKVFKRIGLLIIILVVILSGFLLFRTTRFTSKQVSVQSVPL
                     AVKDQDTTARHLASALQFQTISILEADQFRQFHEFLKATYPKVFATLTLEKVNEHSLL
                     FRWEGRAPNLKPILLVAHQDVVPVEPGTEDNWTHPPFGGHIADGYVWGRGSLDDKSGV
                     IGILEAAELLLSEGFQPQRTMYFAFGHDEETSGKQGAAEISKVLQSRGLQFECVLDEG
                     GAVVEDYPQVPLPVALVGIAEKGYVSIKLTAKGEGGHSSMPPKQTAVGILSAALVKLE
                     ANQVPARLEGPTRQMFEYLGPEMSFPNRVIMANLWLFRPLVQSILAKAPTTNAYIRTT
                     TAPTMLEGSIKDNVLPISARAIINFRILPGDTIDSVTRHVRQTVNDPRVDVEPLTEMA
                     ANPSPTSRTDSEAFATVQRTLHQVFPGIVVTPTLVIGATDGRYYASLSQNVYRFLPIW
                     IKQADTVRIHGTNERVSVKNFAQAVTFYRQLMVNFDGKTSHSG"
BASE COUNT          364 a          426 c          421 g          397 t
ORIGIN      
        1 ggttccgggt tccgggttcc gggttccggg ttccgggttc cgggtcttcg aattttggtc
       61 ctttttgtcc cttttgtcct ttatgtcttt taaatcctgc cccctgaccc ctaaccgcta
      121 tgactggttt taccatcaaa attcaccatc agttggcgat aaaaagtgac ggcctgggca
      181 aagtttttga ctgacactcg ctcgttggtg ccgtggatgc gaaccgtatc ggcctgtttg
      241 atccagatgg gcaaaaaccg atacacattc tggctgagcg acgcgtaata gcgcccatcc
      301 gttgcgccaa tgaccaatgt cggcgttacg acaattcctg gaaatacctg gtgaagtgtg
      361 cgttgaacgg tggcaaacgc ttcggaatca gttcgtgaag tcggcgaggg gttggctgcc
      421 atttccgtca acggttcgac gtcaacccgt gggtcgttca ccgtctggcg aacgtgtcga
      481 gtcactgaat caatggtgtc tcccggtaaa atccgaaaat tgataatcgc ccgggctgaa
      541 atcggcagca cgttgtcttt gatactgcct tcaagcatcg tcggcgccgt tgtggtgcgg
      601 atataggcat tggtggtcgg tgctttggcc agaatggatt gtaccagcgg gcgaaacaac
      661 cacaaattgg ccataatcac ccggttggga aaagacattt ccgggccgag gtattcaaac
      721 atctggcggg ttggtccttc gagccgggcc gggacctggt tggcttccag tttgacaagt
      781 gccgcgctca ggattccgac cgccgtttgc ttcggaggca ttgacgaatg tccgccttcc
      841 ccttttgcgg tcaacttgat gctgacatag cctttttcag caataccaac cagcgccacc
      901 gggagtggca cctggggata atcttcaacc accgcaccgc cttcatccag aacacattca
      961 aactggagcc cccgcgattg gagaaccttc gaaatctcag ccgccccctg tttcccgctc
     1021 gtctcttcat cgtggccaaa agcaaaatac atcgtgcgct ggggttgaaa tccctcggat
     1081 aaaagcagtt cagccgcttc caagatcccg ataacaccgg acttgtcatc gagtgaacca
     1141 cgtccccaca catatccatc agcgatgtgg ccgccaaacg gaggatgtgt ccagttgtct
     1201 tccgttccag gttcaaccgg gacaacatcc tgatgcgcaa ccagcaggat gggcttcagg
     1261 tttggcgcgc ggccttccca tcgaaacagc aggctgtgct cgttgacttt ttcaagcgtc
     1321 agcgtggcaa acacttttgg ataggtggcc tttaaaaatt cgtgaaactg acggaactga
     1381 tcggcctcca ggattgaaat cgtctgaaat tgcagggctg aggccagatg acgcgccgta
     1441 gtgtcctggt ctttcacagc cagggggact gattgaaccg aaacctgttt tgaagtaaac
     1501 cgggtggttc gaaagagtaa aaaaccactc agaattacca ccaaaatgat gattaagagg
     1561 ccaatgcgtt tgaatacttt ccttttttgc ataatttcag aaaggaac
//
LOCUS       DAOQDO010001730         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85224_length_1608_cov_2.634256, whole genome shotgun sequence.
ACCESSION   DAOQDO010001730 DAOQDO010000000
VERSION     DAOQDO010001730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85224_length_1608_cov_2.634256"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKZ53_22180"
     CDS             <1..267
                     /locus_tag="PKZ53_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43209.1"
                     /translation="GANRKFTQVEMEPAEAKKIAGDLIRVLFQSNRGPILEGLRRGTF
                     SDAIKAEISKAKKEYEKRVPRASRQDFFKQELEEQLRHMKESSR"
     gene            559..>1608
                     /locus_tag="PKZ53_22185"
     CDS             559..>1608
                     /locus_tag="PKZ53_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ43210.1"
                     /translation="MPHDLRHSVANENRPRRQSAWLFGLACLVVFGVAVACATTRSDQ
                     SELVVFVVENSAQLRSGSGREGFQANIKTYLETFPKNRFESELILFGSYAERIVPATV
                     PQITGKSQFADCTAGLGMALNVVKAQPQRVKRVILITSGKQTVNTAEWLNLSQPGMEQ
                     VTPKPSSQTQVPEIARQFAVELCDQLHCPLYIVAIGPSQDSGWLRDMVQAANGNIAAS
                     RILELAVVQLPFRSHLFQQYLYSIEGISSPKQVKQVEGYFKQLAQPVSSSPDIGLGVF
                     GGTVALLVLVQSLRTFPGPGDIEYFEVIEGTPVYLATGIENALALQNNQMAAFSDSVQ
                     MCRGAEMASLGVMYRT"
BASE COUNT          400 a          382 c          423 g          403 t
ORIGIN      
        1 ggggctaacc ggaaattcac ccaggttgaa atggaaccag cggaggccaa aaagattgca
       61 ggtgatttga ttcgagttct gttccagtca aaccggggac caattctcga aggtctgcgg
      121 cgcggaacgt ttagtgatgc cattaaggcc gagatcagta aggcgaaaaa ggaatatgaa
      181 aagcgcgttc ctcgtgcttc gcgtcaagat ttctttaaac aggagttaga agaacaactc
      241 cgccatatga aagaatcttc aagataagca ccgggagggg gatcggggag aaataccttg
      301 tcagaagttg aacgagagtt tggagttttg gacgtggttg agttttttcg tgcttttcgt
      361 agttcaaaat gtcctggaat ttttggtcag gcgtttatct tcgcccccct tcatatcatt
      421 tttgaatcac attctggcag agcaagcagt gaaatagctg gaaacattgg atttcccagc
      481 cctgacccac gaatggtatt agaagtcctg agcctggaaa ccgctcaacc agaacctgga
      541 acctaatttg agggaaccat gccccacgac ttgcggcact ctgtcgccaa tgaaaacagg
      601 ccgcgccgcc aatctgcctg gttgtttggg cttgcctgtc tggttgtttt tggggttgcg
      661 gtggcctgtg ccaccacccg atcagaccag tcagagctgg ttgtctttgt ggtcgaaaat
      721 tcagctcaat tgcggagtgg atctggtcga gaaggattcc aggcaaatat caaaacgtat
      781 ctggagacct tcccaaaaaa tcgatttgag tccgaattaa tcctttttgg gagttacgcc
      841 gaacgaattg tacccgccac ggtaccgcaa ataactggca aatcacagtt tgcagattgt
      901 actgccgggc tgggaatggc cctcaatgtg gtcaaagcgc aaccccagcg agtcaaacgg
      961 gtcatcctga tcacttctgg gaaacaaacc gtcaataccg ctgaatggtt aaatctgagc
     1021 cagcctggca tggaacaggt gactcccaaa ccttcgtctc aaacccaggt tccagaaatt
     1081 gcccgccagt ttgcggttga attatgtgac cagttacatt gccccctcta tattgttgcg
     1141 attggtccga gtcaggactc aggctggctc agagatatgg tccaggccgc aaacggaaat
     1201 attgccgcgt cacggatttt ggaactggcg gtagtgcagc ttccatttcg cagtcacctg
     1261 tttcaacaat acctgtattc aattgaaggg atcagttctc cgaaacaggt caaacaggtc
     1321 gaagggtatt tcaagcaact cgctcagccg gtgtcttcca gcccggacat tggacttggg
     1381 gtatttggag ggacggttgc cttgctggtt ctggtgcagt cgcttcgcac ctttccaggt
     1441 cctggtgaca ttgaatattt tgaagtcatc gaagggaccc cagtctacct ggcaactgga
     1501 atcgaaaatg ccctcgcctt acaaaataac cagatggcgg cgttttctga ttcggtccag
     1561 atgtgccgcg gtgccgaaat ggcttcgctt ggggtgatgt atcgaacc
//
LOCUS       DAOQDO010001731         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85315_length_1607_cov_2.699098, whole genome shotgun sequence.
ACCESSION   DAOQDO010001731 DAOQDO010000000
VERSION     DAOQDO010001731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85315_length_1607_cov_2.699098"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKZ53_22190"
     CDS             <1..387
                     /locus_tag="PKZ53_22190"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNJ43211.1"
                     /translation="NPEPQNGAAMKNLTVLYDINCELCTSIRTWLEKQPKYIEMLFVP
                     AASEFARRRFPDLNHRATLQDLTVVSDKGDVYYGAKGWLMCLWALREYRHWSLKLSAP
                     EYLPTVRRFVSMISRNRHRFRRSSLF"
     gene            407..1177
                     /locus_tag="PKZ53_22195"
     CDS             407..1177
                     /locus_tag="PKZ53_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNJ43212.1"
                     /translation="MGTPAEATPKLSKSEQTRSLILQTALELFRERGYEETTMRAIAE
                     RANISLGNTYYYFKSKEVLIQEFYAHTHGEHVLASAPVLAQEKTLKGRLLGVMKAKLT
                     TIQPYHAFSGVLFKTAADPKSPLNPFSEDSTRTREESTELFAEVLRGAKGKVPLELQS
                     ELANLLWVYHMGVVLFWIHDNSPGCRRTHLLVDHTVDIIVKLIALASTPLLIPLRKKI
                     LKMMTDLRQTAGEIGTPDTLPVELENSEEVEEADPVAE"
     gene            complement(1381..>1607)
                     /locus_tag="PKZ53_22200"
     CDS             complement(1381..>1607)
                     /locus_tag="PKZ53_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43213.1"
                     /translation="SARIIISKSIPPYAIDLLAYNSSTQTNNNGASKNNKTVLKWLEV
                     FFFHKWHPFEKVHRRRVYSYLQADFLAVFN"
BASE COUNT          423 a          402 c          394 g          388 t
ORIGIN      
        1 aacccggaac cccaaaatgg tgctgctatg aaaaatctga ccgtgctcta tgacatcaat
       61 tgtgagttat gcaccagcat tcgaacctgg cttgagaaac agccaaaata tatcgaaatg
      121 ctgtttgtcc cggcagcatc ggagtttgcc cgacgtcggt ttccggattt gaaccaccgg
      181 gcgacactcc aggatctaac ggtggtgagt gacaaagggg atgtttacta tggggcaaaa
      241 ggctggctga tgtgtttgtg ggcgttacgc gaatatcgcc actggtcgtt aaaactttcg
      301 gcgccagagt atttgccaac tgtgcgccgg tttgtttcaa tgatctcgcg caaccggcac
      361 cggtttcgcc gctcatcact tttttgatcc tggaggcaag taacccgtgg gaacaccggc
      421 tgaagcgaca ccgaaacttt cgaaatctga acaaacccgc tcattgattt tacaaaccgc
      481 gctggagctg tttcgcgaac gcggctatga agaaaccacg atgcgggcga ttgccgaacg
      541 cgccaatatt tccctgggca atacttatta ttactttaaa tcgaaagaag ttctgatcca
      601 ggagttttac gcccacaccc acggtgaaca cgtcctggcc agtgcgccgg ttttggccca
      661 ggaaaagacg ctcaagggcc gcctgctcgg ggtgatgaag gccaaactta cgaccatcca
      721 accgtatcac gcgttttccg gcgtgctctt taaaacagcc gccgacccga aaagcccgct
      781 caatccgttc agcgaagact caacccgcac gcgtgaagaa agcaccgaac tttttgccga
      841 agtgctgcgc ggcgccaaag gaaaagtacc gctggaatta caatcagaac tggccaacct
      901 gctctgggtc tatcacatgg gcgtggtgct gttctggatt catgacaatt ctccgggctg
      961 tcggcgaaca cacctgctgg ttgaccacac agttgatatc atcgtgaaac tgattgccct
     1021 cgccagcaca ccgctgctga ttccactccg gaagaagatt ctgaaaatga tgacggacct
     1081 gcgccagacc gccggggaaa ttggaacgcc agacacgctg ccagttgagc tggaaaattc
     1141 agaagaagtc gaagaagcag acccggtggc tgaataatga tgtgatcgga caaacaactt
     1201 ccttgttctc agtctcccta ttctggaagg ggcgattgag cttgacagct tccacccgca
     1261 gggcgatgga aaattccatt gaatcaccct tcagttgagt tctggaaacc tgtgctcatt
     1321 attttggttt tctgtcctac aagaatgtag agtcagaata ctatcaactc attgctacaa
     1381 tcaattgaac actgcaagga aatcagcctg aaggtaggaa tagacccttc ttcgatgcac
     1441 tttttcaaat gggtgccatt tatgaaagaa aaaaacttca agccatttca aaaccgtctt
     1501 attatttttg ctggctccat tattgtttgt ttgtgtgctg gaattataag ccaacagatc
     1561 tatagcatag ggcggaatgg atttggaaat aatgatacga gcgcttt
//
LOCUS       DAOQDO010001732         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85528_length_1605_cov_2.434194, whole genome shotgun sequence.
ACCESSION   DAOQDO010001732 DAOQDO010000000
VERSION     DAOQDO010001732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85528_length_1605_cov_2.434194"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="PKZ53_22205"
     CDS             complement(<1..1564)
                     /locus_tag="PKZ53_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43214.1"
                     /translation="MRHLVPPNMQAIHLIHKILITPSEVFFRPEDQIVRWDGHQITLF
                     NPTTSEDWYGRAFHIGDRIVVRHQSHGLIEWKQGKFELIPGSERFGQLGVYTILPFDS
                     NRWLLFTVQGMMLYDGLAFTAFAPEMTAFLKAVLPYGGLVLPDGRFAINTLNNGVVIL
                     NRQGRVDEIINKASGLGDNAVKYLYLDREGGMWAAMETGLARIEISPQLTFFDERSGL
                     QGLVTAITRYRGDLYVATVSGVYTARPAAESSVLELTSLRTPTFLKVEGLSTQCWVFE
                     QVGDQLLVASNDGIYRIDQLKATPVSPISTACLFQSAHFPGKLVVPDRNGHIFLLAWK
                     QNQWAIESELFDIKAEPRVFQEMPTGEWWIATKTNGLKRVIFDSHQPENKTEIRNLQI
                     KDFGIREGLPALEIPYPLIVHTQLLVGTQKGLVEFDPNTQCFKPASGFITEPGVLPII
                     RIALDANGIVWGNFDKDPQIQEEQLVGRWIPQADGSARWQRSQLGLVPDGLVMNFLAE
                     PDQTVWICSVRGL"
BASE COUNT          407 a          417 c          382 g          399 t
ORIGIN      
        1 acaatccccg aacgctacaa atccagacgg tttgatccgg ctcggccaag aaattcatca
       61 ccaggccgtc cggtaccaac cccaactgtg acctttgcca tctggccgat ccgtcagcct
      121 gcggaatcca gcgtccaacc agttgttctt cctggatttg gggatctttg tcaaagtttc
      181 cccatacaat tccatttgca tcaagtgcga ttcgaataat cggaagcaca ccaggttcag
      241 tgataaatcc agatgccggt ttgaaacact gtgtgttcgg atcaaattca accagacctt
      301 tttgggtccc gacgagtaat tgagtgtgaa ctatcagcgg gtaagggatt tccagagctg
      361 gcaatccttc gcggattcca aagtctttga tctggaggtt tctgatttcc gttttatttt
      421 caggctggtg agaatcaaat ataacccgtt ttaaaccatt ggtctttgtg gcaatccacc
      481 attcaccagt gggcatttct tgaaaaactc ttggttctgc tttgatatca aataattcag
      541 attcaatcgc ccactgattc tgtttccagg ccagcaagaa tatatgcccg ttccgatcag
      601 gaaccaccag ttttccagga aaatgggcgg actgaaacag gcaggcggta cttattggac
      661 tgacgggggt tgccttcaac tggtcaattc gataaatccc atcgttgctg gccaccagca
      721 actgatcgcc tacctgttca aaaacccagc attgggtgga aagaccttca accttgagaa
      781 acgtcggtgt gcgcagagaa gtgagttcca gaacggaact ttcggcagca ggtctggctg
      841 tataaacccc tgacacggtt gccacataga gatctcccct gtatcgcgtg atggcggtga
      901 ccagtccctg taacccggaa cgttcgtcaa aaaaagtcaa ttgtggtgaa atctcaatcc
      961 gggccagacc tgtttccatt gcggcccaca tgccgccctc acggtccaga tagaggtact
     1021 tgactgcatt atcgcctaag ccagaggctt tgttgatgat ttcgtccacc cgcccctggc
     1081 gattgaggat gaccacgccg ttgttcaacg tgttgatggc aaatcgtcca tctggcaata
     1141 ccaggccacc atagggcaag acagccttga gaaaagccgt catctctgga gcaaaggccg
     1201 taaaggcgag gccgtcataa agcatcattc cctgaaccgt aaacagcaac cagcgattag
     1261 aatcgaacgg taaaattgta tacaccccaa gctggccaaa gcgttcgctt ccagggatca
     1321 actcaaattt cccctgcttc cattcaatca ggccatgcga ctggtgcctg accacgattc
     1381 ggtcgccgat gtggaatgcc cgcccatacc agtcttccga ggtggttggg ttaaacaggg
     1441 taatctggtg accatcccac ctcacaatct ggtcttcagg tcgaaaaaac acctcggagg
     1501 gagtaatcag gattttatgg atcaggtgaa tggcttgcat atttgggggc accaaatgtc
     1561 gcattgactc atactgtaat tgtccggctg agtcaggttt gagat
//
LOCUS       DAOQDO010001733         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85634_length_1604_cov_2.781795, whole genome shotgun sequence.
ACCESSION   DAOQDO010001733 DAOQDO010000000
VERSION     DAOQDO010001733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85634_length_1604_cov_2.781795"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKZ53_22210"
     CDS             complement(<1..665)
                     /locus_tag="PKZ53_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43215.1"
                     /translation="MKPNSLSQRWLFLAVISVFFCLTSQTTLARLSPRGGEKIKAEDV
                     IAKHLEDLGPAAVRASIKTRRAEGTASFWSLAVRDYRPSGPALFTSDGKKVLLEMIFG
                     LSDYQSERTTFDGNQVWSGYRRPGVRSPLSEYLLPRKVIFQEGLFGGVLSTGWPLLDL
                     SSNKVRLEYGGIKKVDGTPAHLIRYQPKGGSDVMIRLYFDTEHFRHIQTEYEQVFAAQ
                     MGAT"
     gene            complement(1095..>1604)
                     /locus_tag="PKZ53_22215"
     CDS             complement(1095..>1604)
                     /locus_tag="PKZ53_22215"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNJ43216.1"
                     /translation="SPIPQRNGWLVEQIIRSQRGPNGTSEILIDAAVQSMATAGNHYV
                     TLGLSPLSRRADFSQNNINLPHHPLWLRFVFGWVRAHGQRFYNFDGLDAFKSKFQPEW
                     WEPVFAISNEPNFSLQTLYAIASAFTNGTPITTILKALVGAAQTEIRWMIERVNSPEK
                     SQIQPFVIR"
BASE COUNT          426 a          376 c          422 g          380 t
ORIGIN      
        1 gtagcaccca tttgagccgc aaaaacctgt tcatattccg tctggatatg ccgaaagtgc
       61 tcagtgtcga aatacaggcg aatcatgaca tccgaaccgc ctttgggttg atagcggatc
      121 aaatgagccg gggtcccatc cactttctta atccctccat attcgagccg gactttgttt
      181 gatgaaaggt ccaggagcgg ccagccggtt gagagtacgc cgccaaacaa cccttcctga
      241 aaaatcactt ttcggggaag caggtattcg ctgagcggag accggacacc aggccgccga
      301 tagcccgacc agacttgatt tccgtcaaat gtggtgcgct ctgattgata atcactcaag
      361 ccaaagatca tttcgagcaa aaccttcttc ccatctgaag taaatagcgc tgggcccgaa
      421 ggacgatagt cacgaacagc caacgaccag aaactggcgg tgccttctgc ccggcgggtt
      481 ttgattgaag cccgtaccgc tgccgggccg agatcttcga ggtgtttggc gataacgtct
      541 tcagctttga ttttttctcc gccgcgtggg gacagcctgg caagcgtggt ttgagatgtc
      601 aggcagaaaa agacactgat gacggccagg aacagccatc gttgtgagag cgaatttggt
      661 ttcatagacc tatttttgag cgttcaaagg caaagctcat ttcctcaatg aataagatgt
      721 gccgcgcaga gtggtgaccc acgacggcaa aggtggcgga atgggagcat cttctgatct
      781 caaaacccta aacgaagagt tggttgaccg catgacagtc aagctctgga caggagacac
      841 ctgagagatc aggggaaaat gtttctgaga ttctaaatta cgaaaggtac aaaatataca
      901 aaaaataaat ctgtcggatc tccgttgcgg aaaggttgct tgaacttgac ggtcccaccc
      961 agagatctgg cggatttaaa ggtttgtgtg agtataagat aggggagagg aatctcggaa
     1021 atgtcaatca gcagcctcat aaactgggtt tcattgaact ttgggtatgg ttgcctggac
     1081 aaaaaaataa accgtcacct tatgacaaag ggctggattt gacttttttc cggactattg
     1141 acccgctcga tcatccaccg gatctctgtt tgggcagcac caaccagagc tttgaggatc
     1201 gttgtgatgg gtgttccgtt cgtaaaggct gaagcaattg cgtagagtgt ctgcaaggaa
     1261 aaattcggtt cgtttgaaat tgcaaagact ggttcccacc actcaggctg aaatttcgat
     1321 ttaaaggcat caagtccatc aaaattataa aaccgctgcc catgggcccg cacccatccg
     1381 aagacaaacc gaagccaaag tggatggtgt ggcaggttga tgttgttctg ggagaaatca
     1441 gcccggcgtg aaagtggtga taacccaagc gtgacataat ggtttccagc cgtcgccatt
     1501 gactgaactg cggcatcaat cagaatttca gatgtcccgt tcgggcctct ctggctccgg
     1561 atgatttgtt cgaccaacca gccattgcgc tgcgggatcg gaga
//
LOCUS       DAOQDO010001734         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85748_length_1603_cov_2.691860, whole genome shotgun sequence.
ACCESSION   DAOQDO010001734 DAOQDO010000000
VERSION     DAOQDO010001734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85748_length_1603_cov_2.691860"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1076
                     /locus_tag="PKZ53_22220"
     CDS             3..1076
                     /locus_tag="PKZ53_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43217.1"
                     /translation="MTLVSGLSFVVLLFVQQGSVFCGLMARTAKPIEAVGAPIWGTDP
                     NLQSIDDSKPLIDTDLGRVRSAPGVKWAVPLFLRQVQVRLRDGRFQQVRLFGLDNASL
                     IGRPSQMIEGDPASLYLPDAVIIGKAEAERLGSPKIGDTFELNDRLARVVGIADVPRD
                     FLSSPYIFTTYDRALSYTPRQRKQLNFVLASPQDGVSIEQAIKNIEQTTGLKAYTEEG
                     MSWFTIGYYMRNTGIPINIGLSLGLVFVVGMAIAGQTFYAFALQNERYFGALKAMGTR
                     SRTLVAMIIVQSLTVGLVGYGIGAGAGTLIGWLGRNGRLAFYTPPQLLWISFGITIFI
                     CMLSSLISIQRVLRLEPAVVFRS"
     gene            1196..>1603
                     /locus_tag="PKZ53_22225"
     CDS             1196..>1603
                     /locus_tag="PKZ53_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ43218.1"
                     /translation="MSDVIAATLNKVNKSFGLGDTRIHVLKDVELEVRAGELLLLVGP
                     SGCGKTTLLSVLAGILDIDNGEVDVFGKRLDNLSQSKKTDFRRDYIGFIFQQFNLVPT
                     LTATENVAIPLLIAKRPYQESLRKARTYLELVGL"
BASE COUNT          348 a          401 c          445 g          409 t
ORIGIN      
        1 acatcacgct ggtgagcggc ctgtcgtttg tggttttgct ctttgtgcag caaggctcgg
       61 tcttttgcgg attgatggcc cgaacggcaa aaccgattga ggcggttggc gctccgatct
      121 ggggcacgga tcccaatctt caatcaattg atgattcaaa gccgttgatt gacaccgacc
      181 tgggacgggt tcgaagcgct ccgggtgtga aatgggcggt gccgctgttt ttgcggcagg
      241 ttcaggttcg tctgcgcgat ggacggtttc aacaggtgcg gttgtttggg ttagacaatg
      301 cctcgctgat tggacgacct tcccagatga tcgaaggaga cccggcctcg ctctacctcc
      361 cggatgcggt gatcattgga aaagctgaag ccgaacggct cggcagtcca aagatcggtg
      421 acacgtttga actcaatgac cgtctggcgc gggtggttgg aattgccgat gtgccgcgtg
      481 attttttgtc ttcgccttat atttttacga cgtatgaccg ggcgctttcc tacaccccac
      541 gccaacgcaa gcaactcaac tttgtgctgg cttccccaca ggatggggtt tcaattgaac
      601 aggcgataaa gaatatcgaa cagaccaccg ggctcaaagc ctacaccgaa gaaggcatga
      661 gctggttcac gattggctat tacatgcgca acaccgggat tccaatcaac atcgggttgt
      721 ccctgggact ggtgtttgtc gtcggaatgg cgattgccgg gcagacattt tatgcctttg
      781 cactccaaaa tgaacggtat ttcggcgcat taaaggcgat gggcacccgg agtcgaacgc
      841 tggtcgccat gattattgtt caaagtctga cggttggtct ggtcggatac ggcatcggtg
      901 ccggtgccgg gacattgatt ggctggcttg gtcgcaacgg gcggctcgcc ttttacaccc
      961 cgccgcaact gctctggatc agttttggaa tcacgatttt catttgcatg ctgtcgagcc
     1021 tgattagtat tcagcgggtg ctgagactgg agccggcagt tgtgtttcga agctaattga
     1081 aaaccattgg gcttggggct tggggcttgg ggcttagggc ttttcgaaag atcagggttt
     1141 tcagccctca gccctcagcc ctcagccctt aaagaatcct gaaccctata tccctatgtc
     1201 tgacgtcatt gctgccacac tcaacaaagt taataaaagc ttcggcctgg gcgatacccg
     1261 cattcacgtt ttaaaagacg ttgaactgga agttcgggct ggtgaactac tcctgctggt
     1321 tggaccatcc gggtgcggaa agacaaccct gctcagtgta ctggctggaa ttctcgatat
     1381 tgataacggc gaagtagatg tttttggaaa gcgacttgat aatctgtcgc aatccaagaa
     1441 aaccgatttt cggcgggatt atatcggatt catttttcag caattcaacc ttgttccaac
     1501 gctgaccgcg accgagaacg tcgcgattcc gctcttgatc gccaaacgac cttatcaaga
     1561 atcactcaga aaagcccgga cctacctgga actggttggc ctc
//
LOCUS       DAOQDO010001735         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85750_length_1603_cov_2.669897, whole genome shotgun sequence.
ACCESSION   DAOQDO010001735 DAOQDO010000000
VERSION     DAOQDO010001735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85750_length_1603_cov_2.669897"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKZ53_22230"
     CDS             <1..610
                     /locus_tag="PKZ53_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43219.1"
                     /translation="TIGFSAHYTFSKAIDNFLDPIRSDLTTANDSLQPALERGLSVQD
                     VRHRFIASGQWKPNFFRHPVLRDYRIATIIRLESGRPYNLIAGVDLNLNGDFPPADRP
                     LINGVPLGRNVGITPGLAIVDVRLTRTLKLKENINLELIAEGFNLFNHTNINQVNNVF
                     PPDSQANFNLPAKQGSRYSAPPDRWRSAFAARQFQLGFRLSF"
     gene            complement(632..1267)
                     /locus_tag="PKZ53_22235"
     CDS             complement(632..1267)
                     /locus_tag="PKZ53_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNJ43220.1"
                     /translation="MTLLGKLMALYPTFLLSLLLGLCLWFGVRPSILQPCLMVLVLYV
                     LPPVTHRLHSLFFPLREKTSDLSKPEYSPWWGSHQIQVLYIALPGLEALLRLVPGLYS
                     AWLRLWGARIGKNVYWTPLVHITDRTLIEIGDNVLIGHKVEFLCRVIKPNKSRMVLYL
                     KKIKVGNNVFLGAGSRLAPGVVIEDGVYLPILSDIYPNQKVKRSPADTTSE"
     gene            complement(1264..>1603)
                     /locus_tag="PKZ53_22240"
     CDS             complement(1264..>1603)
                     /locus_tag="PKZ53_22240"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ43221.1"
                     /translation="LLADPGPFLMAVALTAALDLWRHTQVTFPAGSRWHRLLARVLVT
                     PLEHQWHHSTTLANRNFGANLSIWDRIHGTYHWSATPPETLGVPVRLSLVRQLIFPFH
                     QASEQENSAR"
BASE COUNT          403 a          421 c          414 g          365 t
ORIGIN      
        1 aacaattggt ttttcagccc attacacgtt ttcaaaggca attgataatt ttctcgatcc
       61 gattcgcagt gatcttacaa ctgccaacga ttcgttacag ccggcactgg aacgagggct
      121 ctcggttcag gatgtacggc atcgctttat tgcctctggc caatggaagc caaatttttt
      181 tcgccatccg gtactgcggg actatcgcat cgctaccatc atccgtttgg aatctggtcg
      241 tccatacaat ctgattgccg gggttgatct gaacctgaat ggcgatttcc ctccggcaga
      301 ccgcccattg atcaatggcg taccgctggg tcgcaatgtg ggtatcacac ccggactggc
      361 cattgtggat gtgcgtctga cccgaacgtt gaaactcaag gaaaacatca atttggaact
      421 gattgctgaa ggttttaacc ttttcaacca caccaacatc aaccaggtaa acaatgtgtt
      481 tccgcctgat tcacaggcga attttaacct gccagccaaa cagggaagtc gctatagcgc
      541 cccacccgac cggtggcgga gcgcctttgc cgcccggcaa ttccagcttg ggttccggct
      601 ttctttctaa atggtgggtt gacatcattc ttcattctga agtggtatca gccggggacc
      661 gtttcacttt ttgatttgga taaatatcag acaaaattgg aagatagaca ccgtcctcga
      721 ttacaacgcc tggtgccagt cggcttccag ccccgagaaa cacattgttt cccaccttaa
      781 ttttcttgag atagagcacc atccgggact tgtttggttt gatgacgcgg cacagaaatt
      841 caaccttgtg accgatcaaa acattgtcgc caatctcaat cagtgtccgg tcagtaatgt
      901 ggaccagtgg cgtccagtag acatttttcc caatcctggc gccccacaac cgcaaccagg
      961 cactatataa acccggcacg agccggagca gggcttcaag ccccggcagg gcaatataga
     1021 gcacctgaat ttgatggctc ccccaccacg gagagtactc tggtttactg agatcagagg
     1081 tcttttcccg cagtggaaaa aacagtgagt gcaggcgatg ggtcaccggc gggagcacat
     1141 acaggaccag taccatcaag cagggttgga gaattgaagg acggacgccg aaccagagac
     1201 ataacccaag cagaagcgac agtaaaaagg tcggatacag cgccatcaat tttcccaaaa
     1261 gcgtcatcgg gcagagttct cctgttcaga tgcctggtgg aatggaaaga tcagttgccg
     1321 aacaagtgac agccgaactg gaacgcccag tgtctcaggt ggcgtggcag accagtgata
     1381 ggtgccgtgg attcgatccc aaattgagag gttggcgcca aaattccgat ttgccagcgt
     1441 tgtgctgtga tgccactggt gttcgagcgg agtaaccaaa acacgggcca gcagccggtg
     1501 ccagcggctc ccggcaggaa aggtaacctg ggtgtgccgc catagatcaa gtgctgccgt
     1561 gagtgccacc gccatcagaa atggacctgg gtcagcgagc aaa
//
LOCUS       DAOQDO010001736         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85751_length_1603_cov_2.668605, whole genome shotgun sequence.
ACCESSION   DAOQDO010001736 DAOQDO010000000
VERSION     DAOQDO010001736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85751_length_1603_cov_2.668605"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1492)
                     /locus_tag="PKZ53_22245"
     CDS             complement(386..1492)
                     /locus_tag="PKZ53_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43222.1"
                     /translation="MLKSLRLKNFTLFPDADLAFGKHLNVIIGENGLGKTHMLKAAYC
                     VLAVSAQGLKDSVSENPTKGHLQKAIANKLRGVFKPDEIGRLARRQAGRNRSEVECRF
                     DDPRFDFRFSFNTSSKTEVAIDSSPAAWMTEVPVYLPTRELLTIAPGFVSLYETTHLQ
                     FEETWKDTVLLLGAPLAKGPREKKIKKLLQPLEEAMDGTVDLDTAGRFYLKTKSGKFE
                     MHLVAEGLRKLAMIARLIATGSLLGKGYLFWDEPEANLNPKLVKLIARVILNISQSGI
                     QVFIATHSLFLMRELYLLQKQHSSDLDARYFGLHRGENEGVNVQPGTTMDEIGDIAAL
                     DEELAQSERYLDTENQLGRLTNFNQAEKSQEFRL"
BASE COUNT          379 a          401 c          395 g          428 t
ORIGIN      
        1 ataaaggtgc gctgaaagaa ggcaccggca tcctgatatt ctcgggtggc cttgccactg
       61 cggaccgccg tcatgtcagc cgcaaattct gattgttgga acgttccttc gagtacgtcg
      121 ggatggggaa cggcgacttc tcgccagggt ttcaggttca tgatcagaat cctttcaagt
      181 tcaagtcaaa caaagacacg aatgatacca gtttgtagtc agtagtcagt agtcagtagt
      241 tcactaagtt catttgattg aattgcttaa ctatctcatc agacgagtat cagcaattct
      301 tagtttggaa atggagagag tcaagagttc cgcctttagg cgggttttgt ctccccaatt
      361 tggtcgaaag ggacactggg gccgcctaaa ggcggaactc ttgacttttt tccgcttgat
      421 tgaagttggt gagtcttcca agctggtttt ctgtgtcaag gtagcgttca gactgtgcca
      481 gctcttcatc aagtgcggca atgtcaccaa tctcatccat tgtagtgccc ggttgaacat
      541 tcacaccttc attctcgcca cggtgtaacc caaaataacg ggcatcaagg tctgaggagt
      601 gctgtttttg gagaaggtac aattcccgca tcaagaaaag gctgtgcgtg gcgatgaaga
      661 cctgaatacc actctggctg atattgagaa tcacccgagc aatgagcttc accagtttgg
      721 ggttcaagtt ggcttccggt tcatcccaga aaagatagcc tttacccaac agagagccgg
      781 ttgcaatcag tcgggcaatc atcgccagtt tcctcagccc ttccgccacc aggtgcatct
      841 caaatttccc cgattttgtt ttgagataaa accggcctgc ggtgtccaaa tccacggtgc
      901 cgtccatcgc ctcttccaac ggctgcaaaa gctttttgat cttcttttca cgcggcccct
      961 tggccagagg cgcccccagc aaaaggaccg tatccttcca ggtttcttca aattgcagat
     1021 gtgtcgtttc gtagagggaa acaaaccccg gcgcaatcgt cagcaattca cgtgttggca
     1081 gatacactgg aacttccgtc atccaggccg ccggtgagga atcaatcgcc acttctgttt
     1141 tgcttgaagt gttgaacgaa aatcggaaat caaaccgggg atcatcaaac cggcattcaa
     1201 cctcgcttcg gtttcgccca gcctggcgac gggctaaacg accaatttca tccggcttaa
     1261 acacacctcg caatttattg gcaattgctt tctgaagatg acctttcgtg ggattttcac
     1321 tcactgagtc tttcagcccc tgagcactca cggccagaac acagtatgcc gcttttaaca
     1381 tgtgtgtctt cccaagcccg ttctctccga taatgacatt gaggtgtttc ccaaatgcca
     1441 ggtcagcatc tggaaaaagc gtgaaatttt tgaggcgaag tgatttcaac atacattttg
     1501 ccagggttca gggttcaggg ttcagggttc agggttcagg gtttttgccg cgtagcggtg
     1561 gttgaactga ggcaggtcgt ttacggcctg caattgaggg ttc
//
LOCUS       DAOQDO010001737         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85838_length_1602_cov_3.053652, whole genome shotgun sequence.
ACCESSION   DAOQDO010001737 DAOQDO010000000
VERSION     DAOQDO010001737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85838_length_1602_cov_3.053652"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKZ53_22250"
     CDS             <1..368
                     /locus_tag="PKZ53_22250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ43223.1"
                     /translation="GARELIAYCQEQFQVGVWSSAKEDYVQALTAQLFDLEKVVVIRH
                     REHCVLVTDDDQFGSGPVKQLRQLEHPIEQVVIVDDDSSTARCNLANLIEVTSFRDNR
                     EGKDLFRVLEELKRRTSHA"
     gene            361..900
                     /locus_tag="PKZ53_22255"
     CDS             361..900
                     /locus_tag="PKZ53_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43224.1"
                     /translation="MPDDQIEIEELADLVVKYAREQNWQLQKPVLTLYGWGWIPTITF
                     WNGMRRVHVYDNIQGSFRDGELWVDGVDGWFVELADRSETQLAPPTIQQCLVRTPEDV
                     WHIVSAFLREEDSVSKLDLFEWTVDTGGAEYHIPHLPNENPSSFGIWPQENQAFRQPP
                     ILSVLFRKVIEWWKKRIDN"
     gene            978..>1602
                     /locus_tag="PKZ53_22260"
     CDS             978..>1602
                     /locus_tag="PKZ53_22260"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNJ43225.1"
                     /translation="MTHFPTFSSQEFQRDLAWARRHMTLTQGQATQLPDLSPVRLAVS
                     AHLDLKTILAIEAVLDHSGSVFLTTCNPTTVRNHVVEYLKSRGAQAHAWNGMSLQDQA
                     EAIEQVLAWNPTHLCEMGADLSGAIIERGLHTQVKAGLEATGSGISRLTRLQSAGNTF
                     TFPIFNWDDLPIKEGLHNRHLVGLTTWHTFTERTRLSLHLKRVLVVGD"
BASE COUNT          412 a          391 c          415 g          384 t
ORIGIN      
        1 caggagcacg ggaattgatt gcctactgtc aggaacagtt tcaggttgga gtctggtcaa
       61 gtgcgaaaga agattacgtt caggcgctca ccgcacaact ctttgacttg gaaaaggtgg
      121 tggtgattcg tcatcgtgag cattgtgttc tggtaactga cgatgatcaa tttggcagtg
      181 ggccggtgaa gcaacttcga cagttagaac atccaatcga gcaggtggtg attgtggatg
      241 acgactcttc gacagcacga tgcaatctgg caaacctgat tgaagtgaca agctttcgcg
      301 acaaccgaga agggaaggac ttgtttcggg tactcgaaga actgaaacgg cggaccagcc
      361 atgcctgacg accagattga aattgaagaa cttgccgacc tggttgtgaa atatgcccgc
      421 gagcaaaact ggcagcttca aaaaccggtc ctcaccctgt atggctgggg gtggattcca
      481 accattacct tttggaatgg gatgagacgt gtccatgtgt atgacaatat tcagggaagt
      541 ttcagagatg gggaactttg ggttgacggg gttgatggct ggtttgtcga actggctgac
      601 agatcagaaa ctcaacttgc tccaccaacg attcagcaat gtctggtcag aacacctgaa
      661 gacgtctggc acattgtttc agctttcctg cgtgaagaag atagtgtgtc aaagttagac
      721 ctttttgaat ggacagttga caccggaggt gcggaatatc acattccaca tctgccaaat
      781 gagaatcctt ccagttttgg gatatggccg caggaaaatc aggcgtttcg ccagccacca
      841 atcctttctg tactctttcg aaaagtcatt gagtggtgga aaaaaagaat tgataactag
      901 ctgaaaaatc tatatttgtc caaactccaa accctgaacc ctgaaccctg aaccccaaac
      961 cccaaacccc aaaccctatg actcatttcc caactttttc cagtcaggaa ttccaacgtg
     1021 atttggcctg ggcgcgtcgg catatgacct tgacccaggg acaggcgaca caattgcccg
     1081 atttgtcgcc agttcggctc gcagtttcag cccatctcga tttaaaaacc attctggcga
     1141 ttgaagctgt gctcgatcat agcggcagcg tttttttgac aacctgcaac ccgacgactg
     1201 ttcgcaatca cgtggttgag tatttaaaat cacgcggcgc acaggctcat gcctggaatg
     1261 gtatgagttt gcaggatcag gccgaagcca ttgaacaggt gctggcgtgg aatccaaccc
     1321 acctgtgtga aatgggggcc gatttaagcg gcgccatcat cgaacgcgga ctccacaccc
     1381 aggttaaagc tggtcttgaa gccacgggct ctgggatttc gcgtctgacc aggctgcaat
     1441 cggctggaaa tacattcacc tttccgattt tcaactggga tgatttgccg attaaagaag
     1501 gtctccacaa ccggcacctg gtcgggctca cgacctggca cacctttacc gagcgaaccc
     1561 ggctcagttt acacctcaag cgcgtgctgg tcgtcggcga tg
//
LOCUS       DAOQDO010001738         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85879_length_1602_cov_2.011635, whole genome shotgun sequence.
ACCESSION   DAOQDO010001738 DAOQDO010000000
VERSION     DAOQDO010001738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85879_length_1602_cov_2.011635"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>1602
                     /locus_tag="PKZ53_22265"
     CDS             139..>1602
                     /locus_tag="PKZ53_22265"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNJ43226.1"
                     /translation="MSSFYVTGGTLRRDAPCYVPRQADIDLLAGLSEGKFCYVLTSRQ
                     MGKSSLMVRTAVRLREEGVAVAVLDLTALGQNLTTEQWYDGLLTRVGQQLDLEDELEE
                     FWLDHERLGPLQRWMRAIREVVLEKIRRQVVIFVDEIDAVRSLPFSTDEFFAALREFF
                     NRRTEDPELTRLTFCLLGVATPSDLIRDTRTTPFNIGRRIELTDFTEAEAAALVRGLG
                     RDEQLGLTLLNRILHWTNGHPYLTQRFCQAVAENQQIQDAPAIDRFCEELFLSSRARE
                     HDDNLLFVRERILRSEADLGGLLDFYARICSRQAVRDDDTNPLINILRLAGIVQIRDG
                     LIVVRNRIYAHVFDQEWVTSHMPDAEIRRQRVAYRRGMIRTTLIAVVILAIMAALLVA
                     AIKQRDRAEQQELSKRRLLYAAQMNLAQQAWEDANVAQVLELLEAYHPKPGQEDFRGF
                     EWYYLWRLCHTNLMTLHHKSLVYAVAFSPDGKWIATGN"
BASE COUNT          320 a          426 c          448 g          408 t
ORIGIN      
        1 ctatctcaaa cgtcatctgc tttaaatcgg ggttccgggt tccgggttcc gggttttcga
       61 aatctttgtc cttttcgtcc ttttcgtcct tttcgtcctt ttcgtctttt aaccctgaac
      121 cctgaaccct aagtttttat gagcagtttt tacgtcacag gcggaacgct acggcgggac
      181 gcaccatgct atgtgccacg tcaggcggat attgacctgc tcgcggggtt gagcgaaggg
      241 aagttctgct atgtcttgac atcgcgacag atgggcaagt cctcgctgat ggtgcggact
      301 gctgtgcggc ttcgcgaaga aggtgtggcc gttgccgtcc ttgatttaac cgccctgggg
      361 caaaacctga ccactgagca atggtatgac ggattgctca cccgtgtcgg gcagcaactt
      421 gatctggaag acgaactcga agaattctgg cttgatcacg aacgactggg accactccag
      481 cgctggatgc gggcaattcg ggaagtcgtc ctggagaaaa tccgccgcca ggttgtgatt
      541 tttgttgatg aaattgatgc tgtccgcagc ctcccgtttt cgaccgatga gttctttgcc
      601 gccctgcgtg aattttttaa ccgccgaacc gaagaccctg aactgacccg tctcaccttt
      661 tgtctgttag gtgttgccac accttccgac ctcattcgcg atacccgcac caccccgttt
      721 aatattggcc gccgtatcga actcaccgac tttactgaag ccgaagctgc cgcactggtc
      781 cgtgggctgg ggcgtgacga gcaactgggc ttgaccctgc tcaaccggat tttgcactgg
      841 actaacgggc atccgtacct gacccaacgg ttttgtcagg ctgtggctga aaaccagcag
      901 attcaggatg cgcctgcgat tgaccggttt tgtgaagaac tgtttttaag ctcacgggct
      961 cgcgaacacg atgacaacct gctttttgtc cgcgagcgga ttttacgaag tgaagccgat
     1021 cttggcgggt tgctcgactt ttatgcccgc atttgttcac ggcaggcggt ccgggatgat
     1081 gataccaacc cgttgattaa tattttgcgg ctggccggga ttgtgcagat tcgggatggg
     1141 ctgattgtcg ttcgcaatcg aatttatgcc catgtgtttg atcaggaatg ggttacctcg
     1201 catatgccgg atgctgaaat tcgccggcaa cgggtggcct atcggcgagg gatgatccgc
     1261 acgacgctga ttgcagtggt gattctggcg attatggcgg ccctgctggt ggccgcgatt
     1321 aaacagcgcg accgggctga gcaacaggaa ctcagcaaac ggcgactgct ctatgccgcc
     1381 cagatgaatc tggcgcaaca ggcgtgggaa gatgccaacg tggctcaggt cctcgaactc
     1441 cttgaagcct atcacccaaa gccggggcag gaagactttc gtggatttga gtggtattac
     1501 ctgtggcggc tctgccacac caacctgatg accctccatc acaaatcact ggtctatgcc
     1561 gtggcttttt ctccggatgg taaatggatt gccaccggga ac
//
LOCUS       DAOQDO010001739         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_85940_length_1601_cov_2.952135, whole genome shotgun sequence.
ACCESSION   DAOQDO010001739 DAOQDO010000000
VERSION     DAOQDO010001739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85940_length_1601_cov_2.952135"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1601
                     /locus_tag="PKZ53_22270"
     CDS             <1..>1601
                     /locus_tag="PKZ53_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HNJ43227.1"
                     /translation="TTLEAYVTALTQLRGQRGHLISLREVRYIDLSQLEKLEHEVVAQ
                     FDRVSARAVEFLRGDKALQPYHVKIARLVADAEPVTTVLGINPHRATVEKIGAELDLL
                     TEVIGGLKIEDTLVRTKILEDISEVMGGLNRARAIVEARRKELLSRESLAEFGAQFKL
                     FAQSVSSALSLADSPEKCDAQMGTLMLRLEEIESRFSEFDEFQVQLAEKRDDVMEAFQ
                     AKRQLLVEQRQRRAEHTFQAADRVLQGIKRRLSTFKTADDLNAYFAADQMVAKVRELI
                     ETLRKLEDSVKADEVESRLKSTREDALRVLRDRQDLFEGAAVVKFGRHRLSVNTQPLE
                     LTMLPRQGAMMFHLSGTDFFQPVADAGFDATRELWEQSLVSETPEVYRGEYLAFCLLS
                     DAESGSHNLSLEQLRDAELSETGLIGVVRTYAETRYDEGYERGIHDADAALILARVLQ
                     LYRTAGRLRFAPGPRALACLFWAFLDDDSKREQWGRQARSLARLRTVFGSTPAIESLI
                     GELMAAITEFLTANHIPFPQEDIKF"
BASE COUNT          372 a          402 c          465 g          362 t
ORIGIN      
        1 ggacaaccct ggaggcctat gtcacggcgc tgacgcaatt gcgtgggcaa cgcgggcatt
       61 taatttcact tcgggaagtt cgctacattg atttgtccca gttggaaaag ctagagcacg
      121 aggttgtcgc gcagtttgac cgggtcagtg ccagagcggt tgaatttttg agaggtgaca
      181 aggcgcttca gccctatcac gtcaaaattg ccagactggt ggccgatgcg gaaccggtga
      241 ccactgtcct tggaatcaat cctcatcggg ccacggttga aaaaatcggg gccgaactcg
      301 atttactcac cgaagtcatc ggtggcttaa agattgagga cacactggtc aggaccaaaa
      361 tcctggaaga catttctgaa gtcatgggag ggcttaatcg agcgcgggcg attgtcgaag
      421 ctcgccgcaa ggaattgctg tcacgcgaaa gcctggccga atttggcgct cagttcaaac
      481 tctttgctca gagcgtttcc agtgccctca gcctggctga ttcgcctgaa aagtgtgatg
      541 cgcagatggg cacactcatg cttcggctgg aagaaatcga aagccgattc agcgaatttg
      601 acgagtttca ggttcagttg gcggaaaaac gcgacgacgt gatggaagcc ttccaggcca
      661 aacggcaact gctggtcgaa caacgccagc gccgggccga acacacgttt caggctgcag
      721 accgtgtttt gcaggggatc aaacgccgcc tctcgacttt taaaaccgcc gatgacctca
      781 acgcctattt cgcggctgat caaatggtgg cgaaagttcg cgagttgatt gagacgctcc
      841 gcaaactcga agacagtgtc aaagccgacg aagttgaatc acgactgaaa tccacccgcg
      901 aagatgcctt gcgggtcctg cgcgaccggc aggacctgtt tgagggcgcg gcggtcgtaa
      961 aatttggacg ccaccgatta agcgtcaaca ctcagccgct ggaattgacc atgctcccac
     1021 gccagggcgc gatgatgttt catttgagcg ggaccgattt ttttcaacca gtggcagatg
     1081 ccggatttga cgccacccgc gaattgtggg agcaatcact tgtctcggaa acgccggaag
     1141 tctatcgggg tgaatatctc gctttttgcc tgctttctga tgccgaatcc gggagccata
     1201 atttgagtct ggaacagcta cgcgatgctg aattgtcaga aacgggtctc atcggagtgg
     1261 tcagaaccta tgcagaaact cgatatgatg aaggttatga acgcggtatt cacgatgcgg
     1321 atgcggcgct gattttggcc agggtgttac aactctatcg aaccgcaggc cggttgcggt
     1381 ttgcaccggg gccacgggcg ctggcctgtc tgttttgggc atttttggat gatgattcaa
     1441 agcgcgaaca atggggacgt caggcgcgaa gcctggcccg gttgcgaacg gtgtttggtt
     1501 ccactccggc gattgaatca ctcattggcg agttgatggc agccataacc gagtttttga
     1561 cggccaacca tattccgttt ccccaggaag acatcaagtt t
//
LOCUS       DAOQDO010001740         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86025_length_1600_cov_3.699029, whole genome shotgun sequence.
ACCESSION   DAOQDO010001740 DAOQDO010000000
VERSION     DAOQDO010001740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86025_length_1600_cov_3.699029"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="queC"
                     /locus_tag="PKZ53_22275"
     CDS             complement(<1..569)
                     /gene="queC"
                     /locus_tag="PKZ53_22275"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNJ43228.1"
                     /translation="MSKPAVVLLSGGLDSTTVLAIAQSEGYAVYALSFDYGQRHAREL
                     EAARHIAAQSQVKDHLVVSFDMRAIGGSALTAAIDVPKNRSSEQLSAGIPVTYVPARN
                     TIFLSFALAWAEVLGAEDIFIGVNALDYSGYPDCRPEYIHAYETLANLATRAGVEGKQ
                     HLQIHAPLMTLTKAEIIRRGLELGVDYGLT"
     gene            complement(603..>1600)
                     /locus_tag="PKZ53_22280"
     CDS             complement(603..>1600)
                     /locus_tag="PKZ53_22280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNJ43229.1"
                     /translation="LRQGIRFDANVPLPEPTGRNWDELASLVSHPSWLLTHWARQWSP
                     ARAIQIARSDNTIPNFYLWLNPLRAPIDQTRQTLEQAGVQLQTAGNTPQTVQWMEGDF
                     SALWPLVEQGAAYVQDAASQQVARWLGVQPGMRVLDCCAAPGGKTAQLAAQMNNRGEL
                     IACDLHPRRVADLKNNCERLGVTILTTGKVDFSQETLFATAPKYLHLEPAGFDAVLVD
                     APCSGTGTLRRHPEIKWKLTPQKLPELAELQGRILENAAQAVKPGGVLLYATCSLEEK
                     EGEEVVRRFRNRHPEFELEPPADAGSALTSEKYLRTWPDAGPDGFFGARFRKRKH"
BASE COUNT          340 a          431 c          473 g          356 t
ORIGIN      
        1 gtaagtccgt agtctacacc aagttcaagc ccgcgccgaa taatttcagc cttcgtcagt
       61 gtcatcaacg gagcgtgaat ctgcaagtgt tgttttcctt caaccccagc ccgcgtcgcc
      121 agattggcca gcgtttcata ggcgtgaata tattctggcc ggcaatcagg ataacctgaa
      181 taatcgagtg cgttgacccc gatgaaaatg tcttccgcgc ccaggacttc ggcccaggcc
      241 agggcaaatg acagaaaaat ggtatttcgg gcgggcacgt aggtcaccgg aatcccggcg
      301 gagagttgtt cgctggaacg gtttttggga acatcaatcg ctgccgtcag ggcagagcca
      361 ccgatggcac gcatatcaaa tgacaccacc agatggtctt tgacctgtga ttgggccgcg
      421 atgtggcggg cggcttcaag ttcacgggcg tggcgttgtc catagtcaaa tgacaacgca
      481 tagacggcat agccttctga ttgggcaatc gcgagaacgg tggtcgaatc aagaccacca
      541 ctgagcaata caacggctgg tttggacata aatctatgga gtgctccggc ttgacggagc
      601 tttcaatgtt tccgctttcg aaaccgggca ccaaaaaatc cgtctgggcc agcatcgggc
      661 caggtgcgca ggtatttctc tgaggtcagg gctgagcctg catccgctgg cggttcaagc
      721 tcaaattccg gatggcgatt gcgaaaccgg cggacgactt cttcgccttc tttttcttca
      781 agtgagcagg ttgcatagag cagcaccccg ccgggtttga ccgcctgagc cgcattttca
      841 agaatccggc cctggagttc agccagttcg ggcagctttt gcggcgtaag tttccatttg
      901 atttcgggat gtctgcgaag cgtgcctgtg cccgagcaag gcgcgtcaac cagcacggca
      961 tcaaatccag ccggttccag gtgcagatac tttggtgcgg tggcaaagag tgtttcctga
     1021 ctgaaatcaa cttttccagt cgtcagaatc gtgacgccga gccgttcgca attgtttttc
     1081 aggtcagcca cccgtcgcgg atgcaggtca caggcgatca attcgccacg attgttcatc
     1141 tgggccgcca gttgggcggt ttttccgccg ggggcggcac agcaatcaag cactcgcata
     1201 ccgggctgga cgccgagcca gcgggctacc tgttgcgagg cggcatcctg aacgtaggcg
     1261 gcaccctgtt cgaccagcgg ccagagggct gaaaaatcac cttccatcca ctgaacggtt
     1321 tgcggggtgt tacctgcggt ttggagctga actccggctt gttcaagggt ttgccgtgtt
     1381 tgatcaatcg gcgcccgcag gggattcaac cagagataga aattgggaat ggtattgtca
     1441 gacctggcaa tctggatggc ccgtgccggt gaccactggc gtgcccagtg ggtgagcaac
     1501 cacgatggat gcgacacgag gctagcaagc tcatcccagt tgcgtccggt tggttccggc
     1561 agcggtacgt tcgcatcaaa gcgaataccc tggcggagtg
//
LOCUS       DAOQDO010001741         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86030_length_1600_cov_3.500971, whole genome shotgun sequence.
ACCESSION   DAOQDO010001741 DAOQDO010000000
VERSION     DAOQDO010001741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86030_length_1600_cov_3.500971"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..390)
                     /locus_tag="PKZ53_22285"
     CDS             complement(43..390)
                     /locus_tag="PKZ53_22285"
                     /inference="COORDINATES: protein motif:HMM:NF036463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HNJ43230.1"
                     /translation="MYTAVIPREEWVNFFNSFSCEHHGWLVSMEIFTDEIGAQVEAKN
                     LPLAGIAVDIKGSGANNILITVGEKAGAQVTHTIRSPKQVCLERSNGGCDESLQIESV
                     SGEQTLLRFCGAD"
     gene            complement(617..>1600)
                     /locus_tag="PKZ53_22290"
     CDS             complement(617..>1600)
                     /locus_tag="PKZ53_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ43231.1"
                     /translation="ISAIDVPPIAMTAIMAAKLYTKRHEIGVLRHSYSGRSATSLSAM
                     GHAPWRLLPTQVAGFVHAAAPYCYRCPFKLTYPSCELACANDIEELIRTTTSGQIAAF
                     MAEPILGVGGFVVPPPGYFERAAEIARQYGGLFIADEVQTAWGRTGDKWFGIEHWNVE
                     PDIITSAKGLGNGAPIGLTIATPEVADAVPSPTFSTFGGNPVSMAAALAVIKTIEEED
                     LRTHVKVVGTYLREKLDGLKEKYAIIGDVRGMGLMLGLELVKDRTTKEPNPQGLAEVF
                     EETKKRNVLIGKGGLYGNVIRTGMPMIASKDNVDELVEALDASFAAVSNRS"
BASE COUNT          368 a          415 c          421 g          396 t
ORIGIN      
        1 gactactgac tactgactac aaactggtat tctaagttcg ctttaatccg ctccgcaaaa
       61 ccggagtaag gtttgttcac ctgagacaga ttcaatttgt aaagattcgt cgcacccgcc
      121 gtttgatcgt tcaagacaaa cttgctttgg gctccgaatg gtatgcgtca cctgagcgcc
      181 ggctttttcg ccgacggtaa tcaggatgtt attggcaccg cttcctttga tatccactgc
      241 aatccccgcc aacggcaaat tctttgcctc gacttgcgcg ccgatttcat cggtgaaaat
      301 ctccattgaa accagccagc catgatgctc gcaactaaag ctattaaaga aattaaccca
      361 ttcttcgcgt gggataaccg ctgtatacat agacactcgc tttcttgccg ctgcgaaggc
      421 aattgatgaa ctgtggattt ttcagattgg atgcctgcaa ttcttcaata gagcaaacac
      481 tatgccaggg tgaaacgagc gggatatcac cggtttgggg ggaatatggg gtgtgaaatg
      541 aggaaaaatt cagtcggcgg gaaaaagtgc gcaatttgtg gagtgcggcg gcttgacgcc
      601 gcactccaca ccagaatcag gatcggttgg aaactgccgc aaacgatgcg tcgagtgcct
      661 cgaccagttc gtctacgttg tccttagaag caatcatcgg catgccggtg cggatgacgt
      721 ttccgtacag tccgcctttt ccgatcaaga cgtttcgttt cttggtttct tcaaagactt
      781 cggccagtcc ttgcggattg ggttccttgg tggtgcggtc tttgaccaac tccaggccga
      841 gcatcaggcc cattccccga acatccccaa tgatggcgta cttttctttg aggccgtcga
      901 gtttttcacg caggtaggtg ccgaccactt tgacgtgggt gcgcaaatcc tcttcttcaa
      961 tggttttaat cacggccagg gctgcggcca tcgaaaccgg gttgccgcca aaggttgaaa
     1021 atgttggact tggaacggca tctgccactt caggcgtggc aatagtcaac ccaatcgggg
     1081 cgccatttcc caggcctttg gcggaggtga tgatgtcagg ttccacattc caatgttcaa
     1141 ttccaaacca tttgtcaccc gttcgccccc aggcagtctg aacttcgtcg gcaataaaca
     1201 aaccaccata ttgtcgggca atctcggcag cgcgttcaaa ataccccggt ggaggaacga
     1261 caaatccacc gacgccaaga attggctctg ccataaaggc tgcaatttgg ccggaagttg
     1321 tggtccggat gagttcttcg atgtcattgg cacaggccag ttcacagctt gggtaggtca
     1381 atttgaatgg gcaccggtag caatatggcg ctgccgcgtg aacaaacccg gccacctggg
     1441 tcgggagcaa tcgccagggc gcatgaccca ttgccgaaag cgatgtggcc gagcgtccgc
     1501 tgtagctatg gcgtaacacc ccgatttcat ggcgtttggt atagagtttg gcggccataa
     1561 tcgctgtcat ggcgatgggt ggcacgtcga tagctgaaat
//
LOCUS       DAOQDO010001742         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86064_length_1600_cov_2.372816, whole genome shotgun sequence.
ACCESSION   DAOQDO010001742 DAOQDO010000000
VERSION     DAOQDO010001742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86064_length_1600_cov_2.372816"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..>1600
                     /locus_tag="PKZ53_22295"
     CDS             235..>1600
                     /locus_tag="PKZ53_22295"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNJ43232.1"
                     /translation="MSSPADSLPSVNDPNETPLDLEESKKTQAKKTSGKSPIDVFLDF
                     LSSVKVGVTLLAFLILSTAIGTFIIQKGTSGFREYYQQLLPAEKTLYTFLGFLDIYHT
                     WWFNLLLLTVSLNIILASIDYFPAAWRYVKKPVLKINQAFINRQPWKDSVVVPTTSVD
                     LNKIKDACEHVFLPGWAKGPLRWVTSVRLFVTENPQHKSTTILVERGVWNRLMPYAIH
                     VALLTIFIGAFIGGQLGRKGVIRLAPGDTTNRFITQSPDPAIPDDEFEMPFTLVCTDI
                     QQDLLNPKKNDLSAPNTLDWHTKVLFQYKGQNYAGHIHLNTPIDFRGYRFFQASFDNV
                     ASAREVSLILTPTTGGTPQEVKISREATKVPGVGTLRLADFYPDFMFSNGQVGTRSGE
                     YNNPVAELEWIPESGGAPSLVLAFNAEMAKQVENLPVGNRTLAGTVRTSLKDFEKVSR
                     AHVLQ"
BASE COUNT          390 a          434 c          358 g          418 t
ORIGIN      
        1 tatcccccat tccctatccc ctatcctcca tcccctatcc cccatcccct atcctccatc
       61 ccctatcctc catcccctat ctcctacctc tttgtcatga tgtgggacat ccgtcttgat
      121 cttctgagtc tcaggcgtca ataatcaaag tttcccagtt ctcaagatgc accccaggtg
      181 tggaaagctc tatcccaaca cgttttcatc actttaagtc aggttatttg tcttatgtca
      241 tcaccagccg attcgctccc ttctgtcaac gatccgaatg aaacaccact tgatctggaa
      301 gaatctaaaa agacccaggc caaaaaaaca tcaggaaaaa gcccaattga tgtgtttctt
      361 gattttctga gctctgtcaa agttggagtt acactgctgg cttttttgat tttatccacg
      421 gcgattggga catttatcat ccaaaaaggc accagtgggt ttcgtgagta ctatcagcaa
      481 ttgttaccgg cggagaaaac cctctacacg tttcttggct ttttggatat ttatcacacc
      541 tggtggttta acctgctgtt gctgacagtc agtttgaaca tcattctggc ttcgattgat
      601 tattttccgg cagcctggcg ctatgtgaaa aaaccagttc tgaaaatcaa ccaggcgttt
      661 atcaaccgtc agccctggaa agattcagtc gtggtgccga ccacctcagt tgacctcaat
      721 aaaatcaagg atgcctgcga acatgttttt ttacccggtt gggccaaagg accattgcgt
      781 tgggtgacca gtgttcgact gtttgtgact gaaaatccac agcataagag cacaacgatt
      841 ctggttgaac gcggagtctg gaatcggttg atgccctatg ccatccacgt tgcactgctc
      901 acgatcttta ttggtgcctt tattggcgga caacttggcc gcaaaggggt gatccggctg
      961 gcgccaggtg atacaactaa ccggtttatc acccagagcc cggatccagc catcccagat
     1021 gatgaatttg aaatgccatt cacactggtg tgcactgata ttcagcagga tttactcaat
     1081 cccaagaaaa atgatttgag cgcgcccaac accctcgact ggcacaccaa agttctgttt
     1141 cagtacaagg gccaaaatta cgctggccat atccatctca acacgccgat tgattttcgt
     1201 ggctatcggt tcttccaggc cagctttgac aatgttgcca gcgcccgtga agtctcactg
     1261 atactgacac caaccactgg cggcacgcct caggaagtca aaatcagccg tgaggcaacc
     1321 aaagttccgg gtgtcgggac gttacgtctg gctgactttt acccggattt tatgtttagc
     1381 aacggacagg ttggaacccg ctcgggcgag tacaacaacc cggtggcaga actggaatgg
     1441 atccctgaat caggcggggc gccaagcctg gtcctggcct ttaatgccga aatggccaag
     1501 caggtcgaga atttaccggt tgggaatcga acactggcag ggactgtccg cacctcgctc
     1561 aaggattttg aaaaggtctc gcgcgcccac gtcctgcagg
//
LOCUS       DAOQDO010001743         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86115_length_1599_cov_3.315415, whole genome shotgun sequence.
ACCESSION   DAOQDO010001743 DAOQDO010000000
VERSION     DAOQDO010001743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86115_length_1599_cov_3.315415"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1599
                     /locus_tag="PKZ53_22300"
     CDS             <1..>1599
                     /locus_tag="PKZ53_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNJ43233.1"
                     /translation="AFTATPKAKTLELFGRRPQPDQPAGPDNLPAPFHLYSMRQAIEE
                     GFILDVLKNYTSYKLAFQLASDGKELNEKEIERSEALKGIMRWVRLHPYNISQKVQIT
                     VEHFRETVSPLLNSQAKAMVVVGSRVEAVRWKLAIDGYIRDQNYPIGTMVAFSGEVKD
                     EESGPDPFTETSKLLNPNLKGRNIRDAFAGTEYQILLVANKFQTGFDQPLLCGMYVDK
                     RLAGLQAVQTLSRLNRAHPGKDTTYILDFVNDPEEILAAFKIYYEAAELAGVTDPNLV
                     YDLRARLDTSGYYDDHEIERVVTVEMNPKASQSELSAALEPVADRLLKRYKAATQALD
                     AAKATASADAAQDAKNEIEELILFKRNLGGFIRIYSFLSQIFDYGNTALEKRAIFFKR
                     LLPLLEFGREREGVDLSKVVLTHHKLKNLGNRDLSLSSGEAGKLQPLTEAGTAEVQEK
                     QKALLDEIIAQLNEIFEGDVSESDQLVYVNHALKGKLLQSATLVQQAKNNTKEQFSNS
                     PDLDTALLNAIMDELSTHTIMSKQA"
BASE COUNT          436 a          411 c          411 g          341 t
ORIGIN      
        1 ggcttttacg gcaacaccca aagccaagac gctggaactc tttggccgcc gtccacaacc
       61 cgaccagccg gctggaccag ataaccttcc ggcgccgttc cacctttatt caatgcggca
      121 ggcgattgaa gaagggttca ttctcgatgt gctcaaaaat tacacgtctt acaaactggc
      181 ttttcagctt gccagcgacg ggaaagaatt gaatgaaaag gaaatcgaac gcagtgaagc
      241 gctcaaaggc attatgcgct gggtgcggct gcatccgtac aatatcagcc agaaagttca
      301 gatcacggtt gaacatttcc gcgaaaccgt gtctcccttg ctcaacagcc aggcaaaagc
      361 catggttgtg gttggcagtc gggttgaggc ggtgcgctgg aaactggcaa tagatggcta
      421 cattcgagat caaaactacc caatcggcac gatggtggct ttttcaggtg aagtcaaaga
      481 tgaggaatcc ggccccgatc cgttcaccga aaccagcaaa ctgctcaacc caaacctgaa
      541 aggccgaaac atccgcgatg cttttgccgg aactgagtat caaattctcc tggtggcaaa
      601 caagtttcaa accggtttcg atcagccgct gttgtgtgga atgtatgttg ataaacggtt
      661 ggcgggactc caggccgtgc agacgctttc acggctgaac cgggcacatc ccggtaagga
      721 cacaacttac attttggatt tcgtcaacga cccggaagaa atcctggcag cgttcaaaat
      781 ttactatgaa gcggcggagc tggcgggtgt caccgatcca aacctggttt atgacctgcg
      841 cgccaggctc gatacgtcgg gttattacga tgatcacgaa attgaacggg tcgtaacggt
      901 tgaaatgaat cccaaagcaa gccaaagcga actttcagcc gcgctggaac cagtcgctga
      961 ccggttgctg aaacgctaca aagcggccac ccaggcgctg gatgccgcca aagcaacggc
     1021 gtccgcagat gctgcccagg atgcgaaaaa cgaaatcgag gaactgattc tgttcaagcg
     1081 caatctggga ggcttcatcc ggatctattc ctttttgtcg cagatttttg attatggaaa
     1141 cacggccctt gaaaaacggg ctattttctt caaacggttg ctgccgctgc ttgaattcgg
     1201 gagggagcgc gaaggtgtgg acctttccaa agtcgtgctc actcaccaca agctcaaaaa
     1261 cctgggcaat cgcgatttgt cgttgtcgtc cggggaagct gggaaattgc agccgctgac
     1321 cgaagccggt actgctgaag tccaggaaaa gcaaaaagcc ttgctggatg aaatcattgc
     1381 ccagttgaat gaaatctttg aaggcgacgt ctcggaaagc gatcaactgg tgtatgtcaa
     1441 ccacgcactc aaaggaaaac tcttgcaatc ggcaacgctg gttcaacagg caaagaacaa
     1501 caccaaagaa cagttttcga attcaccaga cctcgatacc gctttgttaa acgccattat
     1561 ggatgaactc agtacccaca ccatcatgag caaacaggc
//
LOCUS       DAOQDO010001744         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86250_length_1598_cov_2.600778, whole genome shotgun sequence.
ACCESSION   DAOQDO010001744 DAOQDO010000000
VERSION     DAOQDO010001744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86250_length_1598_cov_2.600778"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /locus_tag="PKZ53_22305"
     CDS             <1..>1598
                     /locus_tag="PKZ53_22305"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ43234.1"
                     /translation="RRLKENRERGNIKPALRVIFEASSEIRNSIVYATIIVVLVFIPL
                     FALSGVEGRLFAPLAVAYIVSLTMSLVVSLTITPVLCSYLLPKAKIMEHGESLFVRWL
                     KKLDARLLHITLDHPMVIICGAALLFSVAMATVPFMGREFLPSFNEGSLTINVLSEPG
                     ISLAESDQIGMVAEQLLLTVPEVKTTGRRTGRAELDEHAEGVHYSEIDCDLRKSPREK
                     EEILADIREKLSLLPGVSINIGQPISHRIDHLLSGVRAQVAVKLFGDDLTTLRTRAED
                     IRKVMQAIDGVTDLSVEKQVLIPQLRIAVNREAAGRYGLFPGTVAEQMETLLNGTVVS
                     QVLDGQRTFDVLLRLNEPSRNDFETIKQILIDTPSGAKIPISTVAEIQPEKGPNQILR
                     ENVRRRIVIQCNVAGRDLGSTITEIQARIKNQVPLPTGYFVEYGGQFESQRTATRQLG
                     ILSIFTLIAIFIVLYKALDDWRIALQVMANVPLAFIGGVIAVFATGGVLSVASLIGFI
                     SLTGITVRNGIMMLSHYQHLMREE"
BASE COUNT          399 a          359 c          387 g          453 t
ORIGIN      
        1 tccggcggtt gaaagaaaat cgtgagcggg gaaacatcaa acctgctctg cgagtaattt
       61 ttgaagcgtc gtccgaaatt agaaattcga ttgtctacgc cacaattatt gtcgtactgg
      121 tgtttatccc attatttgct ctttcaggtg ttgaaggccg gcttttcgcg ccacttgccg
      181 ttgcctacat tgtttcattg accatgagtt tggtcgtaag tctcacgatc acgccagtcc
      241 tgtgctcgta tctgcttcca aaagcaaaaa ttatggaaca tggtgaaagc ctgtttgttc
      301 ggtggttaaa gaaactggat gcccgattgc ttcatattac gctggatcat ccgatggtga
      361 ttatttgtgg ggcagccctg ttattcagtg tggcaatggc aacagtgcca tttatgggcc
      421 gcgagtttct tccttcattc aacgaaggct ccctgaccat caatgtactc tctgaaccag
      481 ggatttcatt agctgaatca gaccaaatcg gaatggtggc cgaacaactt ctactcaccg
      541 tccctgaggt aaaaaccact gggcggcgta ctggacgggc cgaattggat gagcatgccg
      601 aaggagttca ttactctgaa attgattgtg acttacgaaa aagcccccga gagaaagaag
      661 agattctggc tgacattcgt gaaaaacttt ctctacttcc tggtgtttcg atcaatattg
      721 gacaaccaat ttcacatcgg attgatcatc ttctgtcagg tgtgcgtgct caagtcgcgg
      781 tcaagttatt tggggatgat ttaacaaccc tccgtaccag ggctgaggat atccgaaaag
      841 tcatgcaagc tattgatggg gtgacagatc tttccgttga aaaacaagtt cttattccgc
      901 aattacggat tgctgtcaac cgtgaagccg ctggacggta tgggttgttt ccgggaaccg
      961 tggccgagca aatggaaacc ttactgaatg gtacagttgt ttcacaggtt ttagatgggc
     1021 aacgaacatt tgatgttctt ttgcgactca acgagccctc tcgcaatgat tttgaaacaa
     1081 tcaaacaaat tctgattgat acaccttcgg gtgccaaaat ccccatttcc actgtggctg
     1141 aaattcagcc tgagaaagga cccaaccaga tcctgcgtga aaatgttcgt cgccgaattg
     1201 taatccagtg caatgttgcc ggacgagacc ttggaagtac gattacggaa atccaggctc
     1261 ggatcaaaaa ccaggttcct ttgccaaccg ggtattttgt tgaatacggt gggcagtttg
     1321 aaagccagcg gactgcaact cgacaacttg gaatcttgag catatttaca cttattgcca
     1381 tttttattgt gctctacaag gcactggacg attggcgtat tgcattgcaa gtgatggcga
     1441 atgtcccact tgccttcatt ggcggtgtga tcgctgtttt tgccactgga ggagtcttat
     1501 ccgttgcgtc actcattgga tttatctctc tgaccggcat tacggttcgc aatggcatta
     1561 tgatgttgtc gcactaccag cacctgatgc gcgaagaa
//
LOCUS       DAOQDO010001745         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86378_length_1597_cov_2.161479, whole genome shotgun sequence.
ACCESSION   DAOQDO010001745 DAOQDO010000000
VERSION     DAOQDO010001745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86378_length_1597_cov_2.161479"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKZ53_22310"
     CDS             <1..577
                     /locus_tag="PKZ53_22310"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43235.1"
                     /translation="LRFNGGVPGVATLLASVGGGFGEVTNPHGLAIDGNFNLYITDRL
                     ASRVLRIQNANTVTLPNLGIPVAQPGNGLSPGQVQSPTGVGVDNDGNLYVADTGNDRI
                     LMFFRGNAGPATLIASGGSNLGEVIQPEGVTVTRFVLGPESGRRNLVVGNSGNHRIES
                     REFGSSVWKLVGTPNLFGSAVGQFNYPSKIR"
     gene            766..1506
                     /locus_tag="PKZ53_22315"
     CDS             766..1506
                     /locus_tag="PKZ53_22315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ43236.1"
                     /translation="MSTREITAVIVDDERMARKMLRPLLAEHPHIQIVGEASNITQAI
                     EVIGETNPEVVFLDIQMPGASGFELLNQIEVTFKVIFVTAFDNFALRAFEVNALHYLL
                     KPVDPEALAQAIHRLTPQAVAESVVPEPVETRLQADDPILLTSGERQRLVKISAIKVI
                     HAEREYSSVVTRDGQRFLMRKSMSDWEAKLSDVGFVRIHRSVIINPDFIDRLEKDEAG
                     NYQAFLVNEPEALPISRRSLAQLRSRLK"
BASE COUNT          370 a          411 c          422 g          394 t
ORIGIN      
        1 gttgcgcttc aacggtggtg ttccaggtgt ggcgacgctg ctggcatcag tcggcggcgg
       61 gtttggcgaa gtcaccaatc cgcatggcct ggcaattgat ggcaacttta acctgtatat
      121 cacagatcgc ctggcaagcc gggtgctccg aatccaaaac gccaacaccg tgacacttcc
      181 aaatctgggc atcccagttg cccaacctgg caatggtctc tcgcccggac aggtccagtc
      241 gccgaccggt gttggtgttg ataatgacgg gaatctctat gttgccgaca ctggcaatga
      301 tcgcatcctg atgttcttca gaggaaacgc agggccggca accctgattg cttcgggagg
      361 gagcaacctg ggcgaggtca tccagccaga aggggtaacc gtgacccgat ttgtacttgg
      421 gccagagtcc gggaggcgaa atctggtggt cggtaacagc ggaaaccacc gcattgaatc
      481 ccgtgagttt ggttcatcgg tctggaaact ggttggaacg cccaacctgt ttggaagcgc
      541 ggttggacaa ttcaactatc caagcaagat tcgatagtct ttgttcagag taacgccttt
      601 aggcgtgagg tttgacgagt gagacgaaag atgagaccct ctcgtctaaa gacgatactc
      661 tgaacttttt tctgcccttg acgcctgcgc ctgccttgaa gactatgtgg atgggtttca
      721 tcatcgtgaa atccatccac attcaggctt tatgggttca aattcatgtc caccagagag
      781 atcacggcgg tgattgttga cgacgaacgg atggccagga agatgctgcg ccccttgctg
      841 gccgaacatc cccacattca gattgttggc gaagcgtcaa atatcaccca ggccatcgaa
      901 gttattggtg aaaccaaccc cgaagttgtt tttctggaca ttcaaatgcc gggcgcttcg
      961 gggtttgagt tgttaaatca gattgaggtc accttcaaag tgattttcgt gaccgcgttc
     1021 gacaactttg cgctcagagc ctttgaagtc aacgccttac attatttgct caaaccagtt
     1081 gacccagaag ccttagctca ggccatccac cgactgacac cccaggcagt tgcggaaagt
     1141 gtcgtccccg agccagtaga aacacgactt caggcggatg atccaatttt gctgacatcc
     1201 ggtgaacgcc agcgtctggt caaaatcagt gcgatcaagg tcatccacgc cgaacgcgaa
     1261 tattccagcg tcgtcactcg cgacggtcag cgatttttga tgcgcaagtc aatgtcagat
     1321 tgggaagcca agttgagcga tgtgggattt gtacggattc accgctcggt gattatcaac
     1381 ccggatttta ttgaccgact tgaaaaagac gaagcgggga attatcaggc atttctggtc
     1441 aacgaaccgg aagcccttcc aatcagtcgc cggtcactgg ctcagttgcg ctcaaggctc
     1501 aaatgatttc agggttccgc gcttccgcgc ttccgggttt tcgaaattaa ttgttcactc
     1561 cttttgtctt tttcatcctt ttagtcacca aactgtg
//
LOCUS       DAOQDO010001746         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86525_length_1595_cov_3.412987, whole genome shotgun sequence.
ACCESSION   DAOQDO010001746 DAOQDO010000000
VERSION     DAOQDO010001746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86525_length_1595_cov_3.412987"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..>1595)
                     /locus_tag="PKZ53_22320"
     CDS             complement(175..>1595)
                     /locus_tag="PKZ53_22320"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNJ43237.1"
                     /translation="ILHFSFFILHSSFSILHSSFISFHRRTYNRSSVLLNSMRILSLG
                     VGISLLLGIAGGANFAYVNGFQSDKPLVTTMECYQVAGKSLQEKDYRTGQKALERALQ
                     LGSSHPRFIYRLAVAQTQVGDTAGALARLKQLAGMQVEYPLETDADLATLKPNPDFQT
                     VVAAFEKNRKPVSQASVAFTIGEKDLIPEGITWDPVDRVYFVGSIYKRKILKIDQAGK
                     ATEFVPSGQDGLGPVIGLKVDPKRRWLWACSAYEKRGDAPLREDGGIFKYDLRTGKLL
                     QKYLLKNQPVAHFLNDLALDAQGGAFVTDSETGSVYVIRPGKSELELFVQSPELIYPN
                     GLALTPDGNQLFVAQYDGIILVDVVTKKLSRLAHPETVTVAGIDGLSLNHRSLIAVQN
                     SFEPHRVIRFDLNSKLNRVERAEVLEARNPHFVIPTTGTMAGNEYVVLANSQLDRLGP
                     DGNLTEPEKLKAVVGLRIRLKK"
BASE COUNT          420 a          398 c          377 g          400 t
ORIGIN      
        1 gactgtgcgg caactgtcag cgaacacagg gcggctaaaa tcaaaagtgc cagaaaacgc
       61 atgacctttc ctcatttata tgttgagagt tccgccttga ggcggcgaca ttgttgattg
      121 gtttcttttt tcaatctttt tccgcctgaa ggcggggcgt tcaactttta caaatcattt
      181 cttgagccga attcgcaacc caaccacagc tttgagcttt tcgggttcgg tcagatttcc
      241 atccgggccc agtcggtcaa gctgactgtt ggccaaaacg acatactcgt tcccagccat
      301 cgtcccggtc gttggaatga caaagtgtgg gttccgggct tcaagaacct cggctcgttc
      361 aacccgattc agtttggagt tcagatcaaa acggatgact cgatgtggct caaaactgtt
      421 ttgaaccgca atcagactgc gatgattgag cgacaggcca tcaatcccgg cgacggtcac
      481 ggtttcaggg tgtgccaatc ggctgagttt tttagttacc acatcaacca ggatgattcc
      541 atcatattga gccacaaaga gttggttacc atcgggcgtc aaggccagac cgtttggata
      601 gatcaactca ggcgattgta caaacagctc cagttcactt ttgccaggtc gaatcacgta
      661 taccgagcca gtttcgctat cggtcacaaa ggcaccaccc tgagcatcca gcgccagatc
      721 attgagaaaa tgagcgaccg gctgattttt caagagatac ttctgaagaa gtttgccggt
      781 cctcaagtca tatttaaaaa tgccgccatc ttcccggagt ggcgcgtcac cgcgtttttc
      841 ataggcacta cacgcccata accagcggcg tttggggtca actttgagac caatcaccgg
      901 acccaggcca tcctgtcctg atggtacaaa ttcggtggct tttccggctt gatcaatttt
      961 caggattttc cgcttgtaaa tgctgccgac aaaataaact cgatcaaccg gatcccaggt
     1021 gatgccctcg ggaatcaagt ctttttcgcc aatggtgaag gctactgatg cctgggaaac
     1081 aggctttcgg tttttttcaa atgccgccac gacggtttga aagtctggat tgggtttgag
     1141 tgtcgccaga tcagcatcgg tttcaagtgg atactcaacc tgcattccag ccaactgttt
     1201 caacctggcc agtgcgccag cagtatcacc aacctgcgtt tgcgcaaccg ccagacggta
     1261 gataaaccgg ggatggcttg acccaagctg caatgcccgc tccagggcct tttgaccagt
     1321 tcgataatcc ttttcctgta agcttttgcc agctacctga tagcactcca tcgtggtaac
     1381 aagtggttta tcactttgaa aaccattgac atatgcgaaa tttgctccac cagctattcc
     1441 caacaacagg ctgatcccga cacccaaaga caaaattctc atagagttca acaaaacaga
     1501 agatcggtta taggtgcgac ggtggaaaga aatgaatgaa gaatgaagaa ttgagaatga
     1561 agaatgaaga atgaagaatg aaaaatgaag aatga
//
LOCUS       DAOQDO010001747         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86633_length_1594_cov_3.333333, whole genome shotgun sequence.
ACCESSION   DAOQDO010001747 DAOQDO010000000
VERSION     DAOQDO010001747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86633_length_1594_cov_3.333333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PKZ53_22325"
     CDS             complement(<1..127)
                     /locus_tag="PKZ53_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HNJ43238.1"
                     /translation="MKPKPGHLGPLLQNFFVDHLCTHKRASPQTIASYRDTFRLLL"
     gene            complement(124..1077)
                     /locus_tag="PKZ53_22330"
     CDS             complement(124..1077)
                     /locus_tag="PKZ53_22330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ43239.1"
                     /translation="MRKTNLLARLDAYLTLREVLGFSPQANRLLLQDFVAYLEEHSDG
                     TPIRAQLAVAWACGSSPARGVSGQAARLRVARAFLTHLKVSLPETEIPPGNLLATPRR
                     PQPFLFSAEEIRRLLAGAARFGPKRSLRPHTHQTLFGLLACTGMRSSEAVNLLVSDVQ
                     LNENPPRLMVRKTKFQKSRWVPLHPSTVEQLQRYAKLRHELNYSGLSDVFFVSEQGQR
                     LDLKILHRTFGRLIYRLDINPRPGQRKPTLHSFRHTFAVNRLRTWYESGVDPRALLPN
                     LSVYLGHLDPASTYWYLTAIPDLLNPAAQLFERYAEKGELK"
     gene            complement(1074..>1594)
                     /locus_tag="PKZ53_22335"
     CDS             complement(1074..>1594)
                     /locus_tag="PKZ53_22335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNJ43240.1"
                     /translation="GSALGRRNFAILMLLSRLGLRAKEVARLQLNDIDWFNGSLLVRA
                     GKTHSERTLPIGQDVGEALLDYVRQGRPNTPHRDIFIEHRAPFRPLQTASAISHIVKR
                     LMVKAGMKRQSSGAHLFRHTAATQMVNRGVSFKEVADVLGHQKLQTTTIYAKLELTAL
                     AEVAMPWPGGVQ"
BASE COUNT          380 a          391 c          457 g          366 t
ORIGIN      
        1 ccagcaacaa ccgaaaggta tcgcggtaac tggcgatggt ttgtggactg gcgcgtttgt
       61 gtgtgcagag gtgatcaaca aagaaatttt gcagcagcgg accgagatga cctggtttcg
      121 gtttcatttg agttctcctt tctctgcata gcgttcaaag agttgcgcgg cggggttgag
      181 caaatctggt atcgccgtga gataccaata ggtggaagct gggtcaagat gtcccaaata
      241 gacggaaaga ttggggagca gtgctcgcgg atcgaccccg gattcatacc aggttctgag
      301 ccgattgaca gcaaaggtgt gacgaaacga atgcaaggta ggttttcgct gccccggtcg
      361 cgggttgatg tcgagtcgat agatcaatcg gccaaaagtt cggtgcaaga ttttgagatc
      421 cagccgctgt ccttgctctg agacgaaaaa aacatccgat agtccgctat agttcaattc
      481 gtggcggagt tttgcatagc gttggagttg ctcgacggtt gaaggatgga gcggaaccca
      541 ccgtgatttc tgaaacttgg ttttccggac catcagacgg ggagggtttt cattgagctg
      601 gacgtctgaa accaaaaggt tgacagcttc gctcgaacgc attccagtac aggccagaag
      661 accgaacaag gtctgatgag tatgtggacg caaggatctt tttggaccga agcgggccgc
      721 cccggccaac agtcgcctga tttcctctgc agaaaaaaga aaaggctgtg gacggcgtgg
      781 tgtcgcaagg agattccctg ggggaatctc tgtttccggc agactgactt tgagatgagt
      841 gagaaatgcc cgcgccactc tgagtcgcgc ggcttgaccg gaaacccctc gtgcaggcga
      901 actcccacag gcccaggcaa ccgccagttg agcccgaatc ggggttccat cactgtgttc
      961 ttccaggtac gccacgaaat cctgtaacag aaggcggttg gcctgtggag aaaaacccag
     1021 aacttcccgc agtgtcagat aggcatcgag tctggccagc aaattggttt tcctcattga
     1081 acacctcctg gccagggcat cgccacttct gctaaagctg tcaattcaag cttggcataa
     1141 atcgtggttg tttgcagttt ctggtgacca agcacatccg cgacttcttt gaagctgaca
     1201 ccccgattga ccatctgggt tgcggctgtg tgtcggaaaa gatgggcacc ggatgactga
     1261 cgtttcatcc cggctttcac cattaaccgt ttgacaatat ggctgatggc tgaagccgtt
     1321 tgcaggggtc ggaatggagc ccgatgttcg ataaagatgt ctcggtgagg agtattcgga
     1381 cggccttgcc gcacatagtc aagcaatgcc tccccaacat cctgaccgat cggaagagtc
     1441 cgctcagaat gtgttttgcc ggcacggacc aacaggctgc cattgaacca atcaatatcg
     1501 ttgagttgaa gtctggcgac ttctttcgca cgcagcccaa gacgggagag caacatcaaa
     1561 atggcaaagt tgcgtcttcc aagcgcgctt ccgt
//
LOCUS       DAOQDO010001748         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86649_length_1594_cov_2.925276, whole genome shotgun sequence.
ACCESSION   DAOQDO010001748 DAOQDO010000000
VERSION     DAOQDO010001748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86649_length_1594_cov_2.925276"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKZ53_22340"
     CDS             <1..443
                     /locus_tag="PKZ53_22340"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ43241.1"
                     /translation="MTDRAWDEVVNTNLNGLFNVTKPVFKQMVRQRYGRILNISSIGG
                     IRTVPTSVHYATTKSAVVGFTKMLAREGAPFGLVVNGIAAGIFDTDLGHTLPDKFRET
                     YEGWCSLGRYGRPEEMGELAAFLVSDRNSYMNGEMIIQDGGTVT"
     gene            701..>1594
                     /locus_tag="PKZ53_22345"
     CDS             701..>1594
                     /locus_tag="PKZ53_22345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ43242.1"
                     /translation="MKLLLSMNLPYLRLNGANKGNRMLAEALSAQGHPFRVIVPSFSP
                     TDQLTRSSFQALLQSRDISFRAEDHKDVFTLNGVEVHSIADPAQLRAELVTELERDEP
                     DFVFISTEDPTQNLLDAALRICPSKVVYIVHTLEFLPFGPHAFFPSQARTRLIAQTAG
                     IVSVSQFVQQYIKEWSGLDSLQLYWPAYGEPPFPHLGSFDNPFVTHINPCAVKGISIL
                     LGLADALPQVQFAAVPTWGATQEDRAALAQRVNISLLTPTEHIDELFRHTKVLLVPSL
                     WFEGLGFVTLEAMLRGVPVIAA"
BASE COUNT          351 a          385 c          429 g          429 t
ORIGIN      
        1 cgatgacgga ccgtgcctgg gatgaagtcg tcaacaccaa tttgaacggc ctgtttaatg
       61 tcaccaaacc tgtgttcaaa caaatggtcc gccaacgcta tggccggatt ttgaatatca
      121 gttcaattgg gggcatccgc accgttccga cgagtgttca ttacgccaca accaagtccg
      181 ccgtggtcgg ttttaccaaa atgctggccc gcgaaggggc gccatttgga ttggtggtca
      241 atggcattgc cgctggaatc tttgacacgg acctgggcca caccttgccc gataaattcc
      301 gcgaaacgta cgaaggctgg tgttcactgg ggcgctatgg acgaccagaa gaaatgggcg
      361 aactggctgc gtttctggtt tctgaccgca atagttatat gaatggcgag atgatcatcc
      421 aggatggcgg gacagtgact taatacatgt tcgggctgaa gaaaccgggc tgaagaagtc
      481 gggctcaggg ttgaagaaac tgaacgtgtt ttttcccagc cagagccggt ggtaagtgct
      541 ctgtcgtaag ttttctgagg tattaaattt ggtaagtctt tgattctttt cgtgtttttc
      601 gtgtttttcg tggttaaaat gtcttggaat ttccggtaga gtacttattg attctgttgg
      661 ggtgaaatac cctaaatcaa aggtaatgtg aggtaatggt ttgaagctgc tgttaagtat
      721 gaatctgccc tatttgcggc tcaatggggc gaataaaggc aatcggatgc tggccgaagc
      781 cctctctgct caaggtcacc cctttcgagt gattgttcct tcctttagcc caaccgacca
      841 gctaacccgc tcatcgtttc aggcgttgct ccagagtcgg gacatttcat ttcgggctga
      901 agatcacaaa gatgtgttta ccctcaatgg ggtcgaagtg cattcaattg ctgacccggc
      961 gcagcttcgg gcagaactgg tcacggaact tgagcgcgat gagcctgatt ttgtgtttat
     1021 ttcgaccgaa gatccaaccc agaatttgct tgatgcggcc ttgaggattt gcccatcgaa
     1081 agttgtgtat attgttcaca cacttgagtt tctcccgttt ggcccccacg cctttttccc
     1141 aagccaggcg cgtacccgtc tgattgcaca aaccgccggg attgtttcgg tcagtcaatt
     1201 tgtgcagcaa tatatcaagg agtggagcgg gctggattcg ctgcaactct actggccagc
     1261 ctatggtgag ccgccatttc cgcaccttgg gagttttgat aacccgtttg tgacgcatat
     1321 caacccatgt gcagtcaaag gcatttcaat tttgctgggt ctggcagatg cccttcccca
     1381 ggttcagttt gccgcggtac cgacctgggg cgctacacag gaagaccggg cggccctggc
     1441 acaacgggtc aatatttcac tcctgacgcc aactgagcac attgacgaac tcttccgaca
     1501 cacaaaagta ttgctggtgc cgtcgttgtg gtttgaagga cttgggtttg tcacacttga
     1561 agccatgctg cgcggggttc cagtgattgc cgcc
//
LOCUS       DAOQDO010001749         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86652_length_1594_cov_2.893437, whole genome shotgun sequence.
ACCESSION   DAOQDO010001749 DAOQDO010000000
VERSION     DAOQDO010001749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86652_length_1594_cov_2.893437"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PKZ53_22350"
     CDS             complement(<1..991)
                     /locus_tag="PKZ53_22350"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ43243.1"
                     /translation="MLKFLASLLVVVCLGVLVPVLAQSPKPLFEIQNYSARFYREQNL
                     SKFSVMAVNHSAEPLTIEVFYELDDENNHAQPVSTQLIELKPGPQKLFYEFDQDQLPA
                     QGFKWKKPFLQEISYRVHLQNGSQFQGGSIELTRPDPEPFVISFISPATACTGTDFHL
                     PVKVLNPTTLAPIAGVRISGTIQYQEDTPDDSINRQLHQEAISDQNGLAMLTFRFEKS
                     IDYDSQLDLEADDGLYQTKTAFNISVETPAQFLLHTDKPLYQPGQTLHVRAQLFDLTN
                     RALPNEKVTLVITNPRQEQMAEHALTASVNGILAFDWEIPAAAPLGNYKIQIKS"
     gene            1206..>1594
                     /locus_tag="PKZ53_22355"
     CDS             1206..>1594
                     /locus_tag="PKZ53_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43244.1"
                     /translation="MNRKPTPGKPYLYVKVEDGTITCTILRDFGDGSGGFDFKGTPFG
                     PEQFQAYRYEFDEEGWRTKLTVLREMGFLPIHEARRQNLIPREWKPLRKDLMISYYII
                     RLLRRFQKIHRLIKRGDLADFRTDDQDL"
BASE COUNT          411 a          338 c          440 g          405 t
ORIGIN      
        1 cggacttgat ttggatttta taattaccca acggtgccgc cgccggaatt tcccaatcaa
       61 acgcaagaat tccattgacc gaagctgtga gtgcgtgctc cgccatctgt tcctgcctgg
      121 ggtttgtaat aaccagcgtc actttttcat tgggcaatgc gcgattggtg agatcaaaaa
      181 gctgagcccg aacgtgcaat gtctggccgg gctggtacag tggtttatcg gtgtgcagca
      241 aaaattgggc tggggtctcg actgaaatat tgaaggccgt tttggtttga tacaacccat
      301 cgtcagcttc caggtccagt tgggagtcat agtctattga cttttcaaag cgaaaggtca
      361 gcattgccag cccgttctgg tcggaaattg cttcctgatg gagctgacgg ttaatcgagt
      421 cgtcaggtgt gtcttcctga tattgaattg ttcctgaaat gcgtactccg gcgatggggg
      481 ccaacgtggt tggattgaga actttaacgg gcaaatggaa atcagtgccg gtgcaggctg
      541 tagccgggct tataaacgag atgacaaacg gttccgggtc aggtcgggta agttcaatgg
      601 aacccccctg gaactgactg ccattttgaa gatgaacccg ataggaaatt tcctgcaaga
      661 acggtttttt ccatttgaac ccctgggctg gcaactgatc ctggtcaaat tcatagaaga
      721 gtttctgcgg tcctggtttg agttcaatga gttgtgtgga gaccggctgg gcgtgattat
      781 tttcgtcgtc gagttcatag aaaacctcga tggtcaacgg ttcagccgaa tgatttactg
      841 ccatcacaga aaacttcgag aggttttgtt cccggtaaaa tcgagcagaa tagttttgga
      901 tttcaaagag tggtttcggt gattgggcca atactggcac caacactcca agacagacca
      961 cgaccagcag gcttgcgaga aacttcagca tatccggtcc cctgaaattg agagataatt
     1021 gcggataaaa accgtttggg acacgctctc aagcttgaac tgattgactc aagagcgcag
     1081 gctattgggc agcataccct ggcagagacg gttctggcaa tgaagttgaa ttgagagcaa
     1141 aaccaacagg caatagatgg tgaattggga tttgtcaaaa cttgtagtca cagaggagaa
     1201 tgaggatgaa ccggaagcca acccctggaa aaccgtatct gtacgtcaaa gttgaagatg
     1261 gaaccattac ctgcacgatt ttgagggact ttggcgatgg ctcagggggt tttgatttca
     1321 aagggacgcc gtttggccct gagcagttcc aggcgtacag atatgaattt gatgaggaag
     1381 gctggcgtac aaagttgacg gtactgcgag agatggggtt ccttccaatt catgaagccc
     1441 gccgtcaaaa cctgattccc agggagtgga aaccgcttcg gaaggatttg atgatcagct
     1501 attacatcat ccggctcttg cggcggtttc agaaaattca caggttgatc aaaagaggag
     1561 atctggctga ttttcgaacg gatgaccagg acct
//
LOCUS       DAOQDO010001750         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86746_length_1593_cov_3.568270, whole genome shotgun sequence.
ACCESSION   DAOQDO010001750 DAOQDO010000000
VERSION     DAOQDO010001750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86746_length_1593_cov_3.568270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="PKZ53_22360"
     CDS             <1..1423
                     /locus_tag="PKZ53_22360"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNJ43245.1"
                     /translation="QFFSEGTLTDDELLKGIRLGVANRSMVPVFVGSAYLNIGIQNLL
                     NALVKFAPSPDVLGEAKGTAPDEPETELTRQVNAKEPFSALVFKTLIDQFNRITLCKI
                     YSGVLKSDSTVFNVTRNTMEKLGSVQAVLGKNLEKLPEACAGDIVALTKLRETMTGDT
                     FCDKAAPIVYTPVVLPEPAIAFALEPKSRTDEDKLSTAIAKILEQDQALRYLRDPQTK
                     EFLLAGSGQLHVEMTVERLKKRYGVDVILHQPKVAYRETFKGSVQVQGRHKKQTGGRG
                     QFGDCVCIFSPAERGEGFKFIDKIFGGSVPANFRPAIEKGIIEAAATGALAGYPMVDF
                     QVELIDGSYHTVDSDEMSFKLAGRKAFRAAMEKVKMVLLEPVMRVEVTAPSEFYGDLI
                     ADMNTRRGRIENTETKGSQVVLKSFVPLSEMLNYMPRLNSITGARGSYTMEFLRYDES
                     PASVVQKVVAEAQAAGRLKVEEE"
BASE COUNT          389 a          402 c          415 g          387 t
ORIGIN      
        1 gcagtttttc tcagaaggca cgctcaccga tgacgaattg ctcaaaggga ttcgacttgg
       61 tgtggccaac cggtccatgg tgccagtgtt tgtcggctcc gcctacctca atatcggaat
      121 ccaaaatctg ctcaatgcac tggtgaaatt tgccccaagt ccagatgtcc tcggtgaagc
      181 caaagggacc gccccggatg aaccggaaac ggaactcacc cgccaggtca atgcgaaaga
      241 acctttttca gcccttgtct ttaagacgct tattgatcaa ttcaaccgca ttactctttg
      301 caaaatttat tctggggtgc tgaaaagtga ttcgacggtg tttaacgtga cccgcaacac
      361 gatggaaaag ctcgggtccg ttcaagccgt actgggcaaa aacctggaaa aactccccga
      421 agcctgtgcg ggcgatattg tggcgctgac caaactcaga gaaaccatga cgggcgatac
      481 cttctgtgac aaagcggcac cgattgtgta taccccggtc gtgttgccgg aaccggcgat
      541 tgcctttgca cttgaaccca aatcccgaac cgatgaggac aagctctcca ccgccatcgc
      601 caaaatttta gagcaggacc aggctctgcg gtatttgcgg gatccacaaa ccaaagaatt
      661 cctgctcgct ggaagtggtc agctccacgt cgaaatgacc gtcgagcgtc tcaaaaaacg
      721 ctatggcgtg gatgtcatcc tgcatcagcc gaaagtggcc taccgcgaaa cctttaaagg
      781 ctcggtccag gttcagggcc gtcataagaa acaaaccggt ggacgcggcc agtttggaga
      841 ttgcgtctgc attttctcgc ctgcggaacg cggcgaaggc tttaagttta ttgacaaaat
      901 ttttggcgga tcggttccag ccaacttccg accggcgatt gaaaaaggga ttatcgaagc
      961 ggcggcgact ggcgccctgg ccgggtatcc gatggttgac tttcaggttg aactcattga
     1021 cgggtcctac cacacggttg actcagatga aatgtcattt aaactcgccg gacgcaaagc
     1081 cttccgggca gccatggaaa aagtcaaaat ggtgttgctg gagccagtca tgcgggttga
     1141 agtcaccgcc ccgagcgaat tttacggtga cctgattgcc gacatgaaca cccgtcgcgg
     1201 acgcattgaa aataccgaaa ccaaaggctc gcaagtcgtg ctcaaatcct ttgttccact
     1261 ttcggaaatg ctcaattaca tgccccgctt aaattcaatc acgggggctc gcggaagcta
     1321 caccatggaa tttttgcgct atgacgaatc tccagcctcg gtggtccaga aagttgtggc
     1381 cgaagctcag gccgctggac ggttgaaggt tgaggaagag taaccttttc gggttccggg
     1441 ttccgggttc cgggttccgg gtttatgtat agcctttcag ataaatattt cctgagtatt
     1501 tgttttggga cgtctcagaa atgattgaag aatttggatt tattttctgt gtgttccgtg
     1561 tattccgtgg tttctttatc tgaaattcta tag
//
LOCUS       DAOQDO010001751         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86861_length_1592_cov_2.988289, whole genome shotgun sequence.
ACCESSION   DAOQDO010001751 DAOQDO010000000
VERSION     DAOQDO010001751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86861_length_1592_cov_2.988289"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1505)
                     /locus_tag="PKZ53_22365"
     CDS             complement(219..1505)
                     /locus_tag="PKZ53_22365"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNJ43246.1"
                     /translation="MEPVVAFSIRDLARSAREASFVAATLSSTTKNNLLRAFALALDQ
                     NREVLTAANARDVEQAERNGIRGAMLDRLRLTDKVISGMMTSLQEVADQTDPVGEILG
                     LTRRPNGIMVGRMRIPLGVIGIIYESRPNVTSDAAGLCLKAGNACILRGGSEAFHSNQ
                     AIGKLFQSVLHQQGLPPALVTIIPTPDRDVLLELLTLEDDIDLIIPRGGESLIRFVAQ
                     HSRIPVIKHYRGVCHTFVDASANPAMATDLICNGKAQRPGVCNATETLLIHKDAVETC
                     LRPMIRNLVQLGVEIRGCEQTILALQGETGLEDKLKAATPEDFGMEFLDLILAVKIVD
                     SFEKAVAHIQQFGSNHTETIVTENHAQAMRFIREINSSTVMVNASTRFADGFQLGLGA
                     EIGISTTRLHAYGPMGTEGLTTLKFVVLGDGQIRTS"
BASE COUNT          378 a          406 c          392 g          416 t
ORIGIN      
        1 tgccatcaac catagtcagt tgggaaaagc ttgtcaagcc gcgctcacca gatccgtggc
       61 ggagataggt gtctatccta ttttggcggt ggctgcttat gagagagttc cagcctgtga
      121 ttctattcct gggtgtaccc tcaagtttca atccaagatc ggtgaaacag ctttgaacca
      181 ccgccaaagt ctattggtct tctggtttgc ccttcgctct aactggttcg aatttgacca
      241 tctccgagga ccacaaattt gagcgttgtg agtccttcag tccccattgg tccataggca
      301 tggagcctgg tggtgctgat cccaatctca gccccaaggc caagttgaaa cccgtcagca
      361 aatcgggttg aagcattgac catcaccgtt gacgaattaa tttcacggat gaatctcatt
      421 gcctgggcat ggttttcggt cacaatcgtc tccgtatgat tggacccaaa ctgttggata
      481 tgggcaaccg ccttttcaaa ggaatccaca atcttgactg ccagaatcaa atcaaggaat
      541 tccatcccaa agtcttcagg ggtagccgct ttgagtttgt cttcaagccc tgtttcccct
      601 tgaagtgcca gtatggtttg ctcacatcct cgaatttcaa ccccaagctg taccaggttt
      661 ctgatcattg gcctgaggca ggtttcaact gcatctttgt ggatcagaag agtttcagtt
      721 gcattgcaaa ctcccggtcg ttgcgctttt ccattacaga tcaggtcagt tgccatggct
      781 gggttggcgc tggcatcaac aaaggtatgg cagacaccac gataatgttt gataaccgga
      841 atccgcgaat gctgggcaac aaaccgaatc aggctctcac ctccgcgagg aatgatcaag
      901 tcaatgtcat cttcgagtgt gagtaactca agcaaaacgt ctcggtccgg cgtagggatt
      961 attgtcacca gtgctggcgg taatccctgt tgatgcaata ccgattggaa cagctttcca
     1021 attgcctgat tggaatggaa tgcctctgac cctccacgta aaatacaggc gttcccagct
     1081 tttaagcaca atcccgctgc atcggaagtg acatttgggc gtgattcata aatgatacca
     1141 atgactccga gcgggattcg cattcgtcca accattattc catttgggcg gcgggtcaag
     1201 ccgagtattt cacctaccgg gtcagtctgg tcagccactt cttgcagtga ggtcatcatg
     1261 cccgaaatga ctttgtcggt gaggcgcaac cgatccaaca ttgccccacg gatcccattt
     1321 cgttcagcct gctccacatc tcgggcattg gctgccgtta acacttcacg attctggtca
     1381 agtgccaggg caaatgcccg gagcaagttg tttttggtgg tactggaaag cgttgcggcg
     1441 acaaaagaag cttcacgagc tgagcgggcg agatcccgaa ttgaaaaggc aacaactggt
     1501 tcaattgaca cggtaaaatc tccttgtggt ggatagctgg gctgaagacg ttgagctcag
     1561 ggtattgaac ccattatctt cagccccgag ct
//
LOCUS       DAOQDO010001752         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_86873_length_1592_cov_2.759271, whole genome shotgun sequence.
ACCESSION   DAOQDO010001752 DAOQDO010000000
VERSION     DAOQDO010001752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86873_length_1592_cov_2.759271"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..913)
                     /locus_tag="PKZ53_22370"
     CDS             complement(266..913)
                     /locus_tag="PKZ53_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNJ43247.1"
                     /translation="MSKPKIELQQIYIQNRQEWRDWLAHNGSTSPGIWLVYYKAGTGK
                     PSIKYTDAVKEALCFGWIDSKAKTIDAERYMQIFTPRKTKSVWSKLNKSYIKELIELG
                     LMAEAGLQKIEMAKADGSWTSLDAIEELVVPPDLQSALETSEAASSYFEQASNSTKKS
                     LLFWVESAKRPETRAKRIAQLVESAAENKHPVDQNSLLVSNSRLSSRNGKVNGPA"
     gene            complement(1076..1468)
                     /locus_tag="PKZ53_22375"
     CDS             complement(1076..1468)
                     /locus_tag="PKZ53_22375"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ43248.1"
                     /translation="MEHPHVELIRRFYTARAENDRDTLREILATDVAWHDPYPPPHGG
                     DLVGIEAVFREIIDKAGQLTGGTTRLWLHDVLANDQHAVALVNWSATYRGKTMEGREV
                     AVYHVHNGQITEAWFYPEDKAASDAFFQ"
BASE COUNT          398 a          358 c          425 g          411 t
ORIGIN      
        1 ggtcaggtcc aactggatgg gtgttccaac tggaactgaa aaacgacctg gaatcaacca
       61 ggtatcatgt ccaaatgctg gcaggacaca aacgatcagg ctgagaaaga taatcaggga
      121 tttaactcgg ttcataaaat cagtttttca ttaggttatg agtaaaggca tacacctgga
      181 caggtttatt gcccagcaac tgttgagtga tgatgtggtt gagttcttta actctgaccg
      241 gggtatgagt tttttggcat tcgtttcagg ctggcccatt gacctttccg ttgcgggaac
      301 tcaacctgga attggaaacc aggagcgaat tttgatcaac cgggtgttta ttctcagcgg
      361 ctgattcaac gagttgggca atgcgtttgg cgcgggtttc cgggcgtttg gcgctttcaa
      421 cccagaagag caggcttttt ttcgtggaat tgctggcctg ttcaaaatag ctgctagccg
      481 cttcactggt ctctagcgcg ctttggaggt caggcgggac gaccagttcc tcaattgcat
      541 cgagcgatgt ccacgagcca tctgctttcg ccatctcgat tttttgcaat ccggcctcag
      601 ccatcaagcc aagttcaatc aattctttga tatagctttt gttgagtttg gaccagacgc
      661 ttttggtttt acgcggagtg aatatctgca tatatcgttc agcatcaatg gttttagctt
      721 tactatcaat ccaaccgaaa cataacgcct ctttgacggc atcagtgtat ttgatactgg
      781 gctttccggt accggctttg tagtacacca gccagattcc cggcgaggta ctgccattgt
      841 gggcgagcca gtcgcgccac tcctggcgat tctggatata aatctgttga agctcaattt
      901 ttggttttga catgaccgag aatccaaaag ggcgagtggg gaaatgcaga ttgctaagta
      961 cttcaccgaa aattccaaga cattttaacc acgaaaacca cgaaaaacac gaaaaacacg
     1021 aaaagaatca aatacttacc aaattcaata tctcaggaaa cttatgacag agtacttatt
     1081 gaaaaaacgc gtccgatgcc gctttatctt ccgggtagaa ccacgcttcg gtgatttggc
     1141 cgttatgaac gtgatagaca gcgacttcgc ggccttccat cgtttttcca cgataggttg
     1201 ctgaccagtt gacgagggcc acggcgtgct ggtcattggc cagcacgtcg tgcaaccaga
     1261 gccgggtggt gccgccggtg agttgtccgg ccttatcaat gatttcgcgg aaaacggctt
     1321 cgattccaac cagatcgcct ccgtgcggag gcggatacgg atcgtgccag gcaacatcag
     1381 tggccaggat ttcgcgaagt gtatcgcggt cattttcagc acgggcggta taaaatcggc
     1441 gaataagttc aacgtgtgga tgttccataa gagagggcca ggggctaggg gctaagggct
     1501 aagggcttga ggttgaagac attgggctga agaagtcggg ctgaagacat cgggctgaag
     1561 acatcgggct gaagaagtcg ggctgaagac at
//
LOCUS       DAOQDO010001753         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87049_length_1590_cov_3.289251, whole genome shotgun sequence.
ACCESSION   DAOQDO010001753 DAOQDO010000000
VERSION     DAOQDO010001753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87049_length_1590_cov_3.289251"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1590
                     /locus_tag="PKZ53_22380"
     CDS             <1..>1590
                     /locus_tag="PKZ53_22380"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43249.1"
                     /translation="PDVLVQFHLNYGSLLMECSQVQEAEHQLQLALKLCQETKLSALG
                     RTQAALARLCNLSGLSEQALEFGRAALDRLQEHEDREGVVAALHTIGEAHLAQGQHAT
                     ALQYLEEALKMADDAGNRAGKAAVLSSLGKMYYQQGRYAQALTQTEQALAITRLLGNG
                     FDERRNLSLLGNIHLYLGQLEEAESYYQSALKMACALGHRLGEAAGLNHIGDVLTRQE
                     RFGEALESYQQAIRIARDIGNRLAEGQYLLNIGAVQRERGEYKAALAALEQALSIAWE
                     TSSHHVEAETLCIIGDTYRRLNLFEESESAFVRAGTLVQELEIPEVAWRTHYFWAISE
                     QAQNKNDRAIKHLQEAITIIERIKAEIPEDDDSSESFKHNKQPVYDLLAKLGGASTSP
                     EVVSKGLSQRMVTPAPITMIMPPKATAPVKDLEFELETDETAWRDESESKGNRSAPVR
                     KGARGSSARLYVEDGGGRTPGREGKAVLSETGEFVDEAFNPVSEVLSDLIEFANQLEM
                     DGLFEVGEVQESSALSRGEAAS"
BASE COUNT          392 a          392 c          461 g          345 t
ORIGIN      
        1 ccagatgtcc tggttcagtt tcacctcaat tatggctcgc tcttgatgga atgctcccag
       61 gttcaggaag ccgaacacca gttgcaactg gcactcaaat tgtgccagga gacaaaactt
      121 tcagccctgg gacgaaccca ggcggcctta gcccgattgt gtaatttatc gggtctttcg
      181 gaacaggcgc tggaatttgg acgggcggcg cttgatcggc ttcaagaaca tgaagaccgc
      241 gaaggtgtcg tcgctgccct ccataccatt ggtgaagccc atctggcgca gggccagcac
      301 gccacggcct tacagtacct cgaagaagcc ttgaaaatgg ctgatgatgc cggaaaccgg
      361 gccggcaagg ccgccgtgct ctccagcctg ggcaagatgt attaccagca aggtcgctat
      421 gctcaggcgt tgacccagac ggaacaggcc ctggctatta cgcggttgct tgggaatgga
      481 tttgacgagc gtcgtaacct cagcttgctc ggaaatattc atctctatct cggccagctt
      541 gaggaagcgg aaagttacta tcaatccgct ttgaaaatgg catgtgcgct gggtcaccgg
      601 cttggtgaag ctgccgggct caatcatatt ggggatgttt taacccggca ggagcggttt
      661 ggcgaagcgt tggaaagcta tcaacaggca attcggattg cacgcgatat tggaaaccgg
      721 ctggcagaag gacagtatct gttgaatatt ggtgcggtgc agcgtgagcg aggcgaatat
      781 aaggcggcac tggccgctct ggaacaggca ctttccattg cctgggagac cagtagccac
      841 cacgtcgaag ctgaaaccct gtgcatcatt ggcgatacct atcgccggct caatctgttt
      901 gaagagtcag aatcggcttt tgtgcgggct gggacgctgg tgcaggaact tgaaatcccc
      961 gaagttgcct ggcggacgca ttatttttgg gcaatttcgg aacaggctca aaacaaaaat
     1021 gatcgggcaa ttaaacacct tcaggaagcc atcaccatta ttgagcggat caaagctgaa
     1081 atccctgaag atgatgattc atcggaaagc tttaagcaca acaaacaacc tgtgtatgac
     1141 ctgctggcca aactcggcgg agcctcaact tcaccggaag ttgtttccaa agggttaagt
     1201 caacgaatgg tcacgccggc acccatcacg atgattatgc cgccgaaggc aaccgcaccg
     1261 gtcaaagatt tagagtttga gctggaaact gatgagaccg cctggagaga cgaatctgaa
     1321 tcaaaaggca accggagtgc cccagtccgc aagggggcac gcggcagcag tgcccggctg
     1381 tacgtcgaag atggtggcgg acgcacacct ggacgcgagg ggaaggctgt cctgagtgaa
     1441 accggcgagt ttgttgatga agcgtttaac ccggtatcag aagttttgtc agacctgatt
     1501 gaatttgcca atcagcttga aatggatggt ctgtttgaag ttggcgaagt ccaggaatcg
     1561 tcggctctct ccagaggtga agcggcttcg
//
LOCUS       DAOQDO010001754         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87197_length_1589_cov_2.358540, whole genome shotgun sequence.
ACCESSION   DAOQDO010001754 DAOQDO010000000
VERSION     DAOQDO010001754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87197_length_1589_cov_2.358540"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..604)
                     /gene="pyrE"
                     /locus_tag="PKZ53_22385"
     CDS             complement(17..604)
                     /gene="pyrE"
                     /locus_tag="PKZ53_22385"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNJ43250.1"
                     /translation="METHDFEHLLTETGALLSGHFRLSSGLHSGNYVQCARLLQFPNI
                     ATQIGQELADKLKTHLTEPPDFVAAPAIGGILIAHEVARAFGVRCLFTEREGTEMKLR
                     RGFLVETGERAIVIEDVVTTGGSTRETMTALEKEGAQIIAVGSIIDRSSEPVNLGIPR
                     ESLIRLQIPSYKPETCPLCQAGMAIVKPGSRKLTE"
     gene            677..1516
                     /locus_tag="PKZ53_22390"
     CDS             677..1516
                     /locus_tag="PKZ53_22390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNJ43251.1"
                     /translation="MIAPVGLVQRLRLKLETASHPVPLETLGRELFFTANGSAQMVCR
                     LLEPLLKEIPEAEVLPNGCVAWLPRKAQPISLRETTYAVVDVETTGSSTRYARVTEIA
                     IYRIERGAIVSEFSTLVNPQMFIPELITSLTGITNAMVAQAPQFADIIEPVAKQLHDA
                     VIVGHNVNFDYSFLNLELALADTQVKLTNPSLCTVKLARTLVPGLENYRLHTVAAHYG
                     IEIKDRHRAGGDAFATARLFLELIDLLDEAGVHTVFEAQRLKRHKRRSYMSGAQRVGS
                     MMN"
BASE COUNT          389 a          399 c          417 g          384 t
ORIGIN      
        1 gtcatcgggt catttgtcat tcggtcagtt tccggcttcc aggtttgacg attgccattc
       61 cggcttgaca cagcgggcag gtttccggtt tataggaggg aatttgcagc cggatgagcg
      121 attcccgagg aatccccagg ttaactggct cactactgcg gtcaatgatt gagcctaccg
      181 caataatctg ggcgccttct ttttcgaggg cggtcatggt ttcacgggtt gatccaccag
      241 tggtgacaac gtcttcgatc acaatggcac gttcacctgt ctcgaccaga aaaccacgcc
      301 tcaatttcat ttctgtgcct tcacgttcgg taaacagaca gcggacgcca aacgcacggg
      361 cgacttcgtg agcaattaaa atccccccaa tcgcaggtgc tgcgacaaag tcaggtggct
      421 cggtcaaatg ggttttcagt ttatcggcca actcctggcc aatctgggtt gcgatgttgg
      481 gaaactgcaa caggcgggca cattgcacat aattgccact gtgcagtcca gaactcagcc
      541 ggaaatgacc gctcaacaga gcaccggttt cagttaaaag gtgttcaaaa tcgtgtgttt
      601 ccatgatttg atgtggtcct tacagcaaaa ttagtgaaaa ctggcctgag gccagagatg
      661 aggaaagctt gaaggtatga tcgcaccagt tggattggtc caacgtcttc gtttgaaatt
      721 agaaactgca tcgcatccgg ttccgctcga aacactgggc cgcgaactct ttttcacggc
      781 taatggttcg gcccagatgg tgtgccgatt gttggagccg ctgctcaaag aaattcccga
      841 agccgaggtg ttgccaaacg ggtgtgtggc ctggctgccg cgtaaagctc agccgatttc
      901 attgcgggaa accacttatg ccgtggtgga tgtcgaaacc actggaagca gcacccgcta
      961 cgcccgcgtc accgaaattg cgatctatcg gatcgagcga ggcgctattg tcagtgagtt
     1021 ttcgaccctc gtcaatccgc aaatgttcat cccagagttg attaccagcc tgacaggtat
     1081 taccaatgcc atggtggccc aggcgcccca gtttgctgac attattgagc ctgttgccaa
     1141 acaactccac gatgcggtga ttgttggcca taacgtcaat tttgattatt catttttgaa
     1201 cctggaactg gcgctggctg atacccaggt gaaattgacc aatccgtcct tatgcaccgt
     1261 aaaactggcc cgaacactgg taccagggct ggaaaattac cggttacaca ccgtcgctgc
     1321 ccactacggc attgaaatca aagatcgtca ccgcgccgga ggcgatgctt ttgccaccgc
     1381 ccggcttttt ttggaattga ttgatttgct ggatgaagcc ggggtgcaca ccgtgtttga
     1441 agcccaacgg ctcaagcgcc ataaacgccg atcttatatg tcaggggccc aacgcgttgg
     1501 ctcaatgatg aattgaacct gcaggtgaca gataccaatg aagaatgaag aatgaagaat
     1561 gaagaatgaa gaatgaagaa tgaagaatg
//
LOCUS       DAOQDO010001755         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87289_length_1588_cov_2.707763, whole genome shotgun sequence.
ACCESSION   DAOQDO010001755 DAOQDO010000000
VERSION     DAOQDO010001755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87289_length_1588_cov_2.707763"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1331
                     /locus_tag="PKZ53_22395"
     CDS             240..1331
                     /locus_tag="PKZ53_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ43252.1"
                     /translation="MKVQRAILPETNRVTWLVLGDNFLPIQPIQSFLTYFQNIERSPF
                     TIRSYAFHLKLYWQFLESTKLDWKKVGLKELSDFVVWLREPHPSGIVAIHERTAKRTE
                     STVNTMLASISAFYTYQERLGVIEPISLHGTLPSHQVGHRGFLHGIAKSKPAKTRLLK
                     LKATKRIPRTLTPDQLGQLVGACGHLRDKFLLCLLSETGMRIGQALGLRHEDILSREK
                     IIRVIPRDDNANGARAKTRESNSIHVSVNLMRLYADYLVDEVQGVRSDYVFINLWHEP
                     TGQPLTTSSVADIFRRLEKKTGIHITPHMLRHTHATELIRSGWDAALVQKRLGHASVQ
                     TTLNFYTHLTDQDLKQAFVDHLKKKEMKP"
BASE COUNT          430 a          382 c          360 g          416 t
ORIGIN      
        1 caggagagcc ggaataacgt ccggctcccg ctgcgcgctc tttgcgccgg tgggttgccc
       61 cccaccgatc ttccttagaa aggaggtcac taacatggat aactatgatt atacatggtg
      121 aataaaatta ctcttaataa aataatcaaa gaatgttttt tattgaattt tgatacttca
      181 tattatacta ttctatcata aatacatgaa gtaaataaag atttgagaaa ctttttctta
      241 tgaaagtaca gcgtgctatc ttgcctgaga ccaatcgggt cacttggttg gttctaggcg
      301 acaattttct cccgattcag ccgatccaat catttctcac ctactttcag aatattgaac
      361 ggtccccttt caccattcgc tcctatgcct ttcacttgaa gctctactgg cagtttcttg
      421 aatcaaccaa actggactgg aaaaaggttg gattaaagga actctcagat tttgtggttt
      481 ggctcagaga accgcatccc tctggaatcg tcgccatcca tgagcgaacc gccaaacgga
      541 ctgaatccac cgttaatacc atgctggctt ccatttcagc tttctacacc tatcaggagc
      601 gattgggtgt gattgagccg atttctttgc acggaaccct cccttcacat caggtggggc
      661 atcgtggttt tctgcatggg attgccaaaa gcaagccggc caaaacccgc ctcctgaaat
      721 tgaaagcaac caaacgaatt cctcggaccc tcaccccgga ccaacttggc caactggttg
      781 gggcttgcgg tcacctgcgc gacaaatttc tgctctgttt gctatctgaa accgggatgc
      841 ggattgggca ggcgctcggg ttgcgccatg aggatattct gtcccgcgag aaaatcattc
      901 gggtgattcc cagagatgac aatgccaacg gggccagagc caaaactcgg gaatcaaact
      961 caatccacgt ttcggtcaat ttgatgcggt tatatgcgga ttacctagtt gatgaagtcc
     1021 agggggtacg gagtgattat gttttcatca acctctggca tgaaccgact gggcagccgc
     1081 tcaccacttc atccgtcgct gatattttcc gccggctgga aaaaaagacc ggcattcaca
     1141 tcaccccgca catgttgcgg cacacccatg cgaccgaact gattcgttcg ggctgggatg
     1201 ctgccctggt ccagaaacgg ctgggacatg cctccgttca gacaaccttg aatttctata
     1261 cccatttgac tgatcaagac ttaaagcaag catttgtaga ccacctgaaa aagaaagaga
     1321 tgaaaccgtg atagtttcaa cgtttagtca atggacagaa gcaaagcaaa acgaactgtg
     1381 caaagaacta accgggtatt gggcagaaaa tatttgggat ttgcgttcgt gtcccgtcaa
     1441 tcttccagtt gcaaatttgg gaaagtgtcg gtttctcaaa tttgagtgcc cctctcaaaa
     1501 tgtcaacaat gaactgaaat acgtatgtcg gaaaaaattt attcagggtg tttggaaagt
     1561 cgggaaagat aagcagaacc tggttcat
//
LOCUS       DAOQDO010001756         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87318_length_1588_cov_2.212655, whole genome shotgun sequence.
ACCESSION   DAOQDO010001756 DAOQDO010000000
VERSION     DAOQDO010001756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87318_length_1588_cov_2.212655"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKZ53_22400"
     CDS             complement(<1..498)
                     /locus_tag="PKZ53_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43253.1"
                     /translation="MKQLTRQVLVSPEAQAQPWQSPALESKIMRHCDLSQLRRWEMLS
                     TRRVLVVMVWLLTIVAGIHIIGTRIPNLVTLRTRRATLFLTLDALKQTFFDQVAGRTA
                     LHDQVAVKPRAVPETWAEPPSFEITIRPHFWHHWWVQLLIGLLFLSIIYAVYAWRIHT
                     LSARQQ"
     gene            980..1189
                     /locus_tag="PKZ53_22405"
     CDS             980..1189
                     /locus_tag="PKZ53_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43254.1"
                     /translation="MSIYPALEFSLSKIEPVHVRKKLLFASNECVWSGMVGSQERDVR
                     QENESSAIPVPDRKTNQKPSFKSKE"
     gene            complement(1401..>1588)
                     /locus_tag="PKZ53_22410"
     CDS             complement(1401..>1588)
                     /locus_tag="PKZ53_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43255.1"
                     /translation="SHSIGKTSRGLIEELDRNRFEVIAIHVPPPRDDAMGRAIRAAAD
                     RNVDLSLTSAPFQFIAP"
BASE COUNT          458 a          358 c          397 g          375 t
ORIGIN      
        1 ttgctggcgt gcactcaatg tatgaatgcg ccaggcatag acggcataaa taattgaaag
       61 gaacaaaagc ccaatcagga gctgaaccca ccagtggtgc cagaagtgag gtcgaatcgt
      121 gatttcaaag gatggaggtt cagcccaggt ttctggcacc gcacggggtt tgaccgcaac
      181 ctgatcgtgc agggcggtcc tgcctgcgac ctgatcgaaa aaggtctgtt tgagggcatc
      241 aagtgtcagg aacaatgtcg ctcgtcgggt acgcaaggtc accaggttgg ggatacgagt
      301 tccaattatg tgaatgccag caacgattgt cagcaaccac accatcacca ccagtaccct
      361 tctggtagac agcatttccc agcgtcgaag ttgcgataaa tcgcaatggc gcataatctt
      421 gctttccaat gcaggcgatt gccagggctg agcctgagct tccggggata ccagaacctg
      481 ccgagtaagt tgtttcaatt agaggttgga agggcgaata caggacaact cttgaaatga
      541 acggtgagct ggaaaggatg aaagtgaaaa ttgaatcaac ccagaatcct ctgaccctgt
      601 ggttttcagc caggagccag caggtcttcc acctgggttg cagagagtgt aatacaaaac
      661 cctgaatcag ccgcgataac tcaccaattc gttcgattcg ttgaataacc agtctgtaat
      721 cttgcagttg ctcagatttg gaggaatgag gcaggaagta aatcgaaagc ttaaaacttg
      781 agatggatac caatagcact ctttttctga aaaaaacctc aataaaagat cattttcctg
      841 aaaattgagg acgcttgctg ggtaaaaatt ctatttcttg agaattttga gaaacttccc
      901 tccaataaac ccgtgaagga tgatcgaggt caatacgcga tcttcagggc tcactttccc
      961 cctacctttc cagttcacta tgagcattta tccagctctt gagttttcac tttcgaaaat
     1021 agaaccggta catgtcagga aaaaactcct ttttgcttca aacgagtgcg tgtggtcagg
     1081 catggtgggc agtcaggaaa gagacgtgag gcaggaaaac gaatcttcag caattcctgt
     1141 accagacagg aaaacaaatc aaaaaccctc ttttaaatca aaagaataaa gcgatttacc
     1201 ttcacaatct ttcaattacg aaagacactt ttttaggcat aaagcacaaa aggtggcatt
     1261 actgcagaca ctttttgttt cattcattca attttgaaaa ccttttgaga aaaacaattg
     1321 cctggactga agaaactcag cccaggcaat tgtttttgaa tgggttgtga agcgggcaaa
     1381 atcacacgcc cgcactcagg ctatggagca ataaactgga acggcgccga ggtgaggctc
     1441 aggtccacgt tgcggtcggc ggccgcgcga atggcacgtc ccattgcgtc gtcccgggga
     1501 ggcggaacgt ggatggcgat gacttcgaac cgattgcgat cgagctcctc gatcagaccg
     1561 cgggaggtct tgccgatgga atgggaat
//
LOCUS       DAOQDO010001757         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87479_length_1586_cov_3.244285, whole genome shotgun sequence.
ACCESSION   DAOQDO010001757 DAOQDO010000000
VERSION     DAOQDO010001757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87479_length_1586_cov_3.244285"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PKZ53_22415"
     CDS             <1..935
                     /locus_tag="PKZ53_22415"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43256.1"
                     /translation="GTGAERRWDGPASASAFAQPSGLATDGQFLFVADSEISSIRSVD
                     TKSGGAVATLAGGDLFDYGDKDGTGDDVRLQHPLGVTFAGGKVFIADTYNHKIKVLNP
                     ATRSVTSLVGSTSPGLEDGEKAKFFEPGGLSEADGKLYVADTNNHAVRVVDLATRKVS
                     TLALKGLKPVSPLPVSSDEFAPNAETITLPVQKIAHGQAGKLILDIGLPGGFHFNDAI
                     EQRYQVKASGTGPLTFAPGDVKKASKFLTFPVSVPFEAAKAGKMELEVSATFYYCRED
                     NTGVCQIKSVIWKVPVEVTASKTSDIRLQFQAKP"
     gene            1039..1479
                     /locus_tag="PKZ53_22420"
     CDS             1039..1479
                     /locus_tag="PKZ53_22420"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ43257.1"
                     /translation="MTAIVDDLKQTIRAASIRLQAYNDKKASQPTAPDKWSRKELLGH
                     LIDSAANNHQRFVRAQFTDGVVLPGYEQNEWVACQQYQSADWLDLVGLWKNYNEHLTR
                     VVAAIPEEKLGNRCQVGQNEPVTLEFLIADYLQHMKHHLGQIFG"
BASE COUNT          419 a          371 c          417 g          379 t
ORIGIN      
        1 ccggtacggg tgcggaacgg cgctgggatg gtccagccag tgcttcagct tttgcccaac
       61 cttcaggact ggcaaccgat ggccagttcc tgtttgtggc tgacagcgaa atcagcagta
      121 tccggtcagt tgataccaaa tcaggtgggg cggtggcgac cctggccgga ggcgatctgt
      181 ttgattatgg cgacaaggat ggaaccgggg acgatgtgcg gttgcaacat ccactcgggg
      241 tgacgtttgc tggcgggaaa gtcttcattg ctgataccta taaccacaaa atcaaagtgt
      301 tgaatccggc aacccggagc gtgacttcgc tggtcggaag taccagccca gggcttgaag
      361 atggcgaaaa ggcaaaattc tttgaacctg gcggactgag tgaagccgat gggaaattgt
      421 atgtggctga taccaataac catgctgtcc gggtggtaga tttagcgacg cgaaaggttt
      481 caaccctggc tctcaaagga ctcaagccgg tgagcccgct gccagtttca tctgatgaat
      541 ttgccccaaa tgcagaaacc atcacccttc cagttcagaa aattgctcac ggacaggctg
      601 gaaaattgat tctggacatt ggactcccag gtggttttca cttcaatgat gcgattgaac
      661 agcgatatca ggtcaaagca agcggtactg ggccgcttac gtttgcaccc ggtgacgtga
      721 aaaaagcgtc taaattcctc acatttccag tcagcgtgcc atttgaggcg gcgaaagccg
      781 gtaaaatgga gcttgaagtt tctgccactt tctattattg cagggaagac aataccggag
      841 tttgccagat caaatccgtg atttggaaag taccggtcga agtcaccgcc agcaagacct
      901 cagacatccg gcttcaattt caagccaaac cctaagtctc aaggaatgat gaatgatgaa
      961 gctgataaac attggccagt caaccagata ggtttattca tcattcatca ttcataactt
     1021 gcatttaaag gagaaccaat gaccgcaatt gttgatgatt tgaaacaaac cattcgggcg
     1081 gcttccatcc ggttgcaagc ctataacgac aagaaagcca gccagccaac ggcacctgac
     1141 aaatggtcac gcaaagaact tttaggccat ttgattgatt cagcggccaa taaccatcag
     1201 cggtttgtcc gggcccagtt tacggacggg gttgtgttac ctgggtatga gcaaaacgag
     1261 tgggtggcct gccagcagta tcaatcggct gactggcttg atctggttgg cttgtggaaa
     1321 aactataacg aacacctcac ccgcgtggta gcggcaatcc cagaagagaa actgggcaac
     1381 cggtgccagg ttggacaaaa cgaacccgta acccttgaat ttttaattgc tgactacctt
     1441 cagcatatga aacaccatct gggacagatt tttgggtagt tgctttccgc actttttcta
     1501 ttttttctcg aaggcgaacc tgaaagggtt cgccttttat aatttcgggc gactcagtta
     1561 tagtcttgga gaggaaaagc ttggag
//
LOCUS       DAOQDO010001758         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87780_length_1583_cov_2.996728, whole genome shotgun sequence.
ACCESSION   DAOQDO010001758 DAOQDO010000000
VERSION     DAOQDO010001758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87780_length_1583_cov_2.996728"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..540)
                     /locus_tag="PKZ53_22425"
     CDS             complement(139..540)
                     /locus_tag="PKZ53_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ43258.1"
                     /translation="MKLTTRSEYALLALIYLGRNQTDGPIASARIAEAQKIPLKYLEQ
                     IMSAMQRSGYVSSIKGQHGGYRLARNPSEISLAQVIRLFDGALAPTESVSKYFYKATP
                     LEKEQKMIDVLREVRDFIAQKLETTTLADLL"
     gene            729..1271
                     /locus_tag="PKZ53_22430"
     CDS             729..1271
                     /locus_tag="PKZ53_22430"
                     /inference="COORDINATES: protein motif:HMM:NF017778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HNJ43259.1"
                     /translation="MSSTVSVDVQTDVLTITDFVNRLTRLDQNERTPDVIAALFQQVQ
                     RFDPEVELHVTFSRQKYTRNLFFRNEAFELLIMCWEPGQGTLIHDHDGSFTVDKIYSG
                     SLQCIDFQRTHLNSSTLEETRRTQLIPGNVMISSSEDIHRVVNISDQTPAVSIHLYAP
                     PLKRMNCFDLETGTTWVVIP"
BASE COUNT          396 a          395 c          349 g          443 t
ORIGIN      
        1 atgcccctcc ttttctatat acaggtgtga aatgagtgtg tgaaattggt taacgtgaca
       61 tccaggttaa cgtgattttc cagggcagag ttcagctttt accaggtgaa cccagccatt
      121 tcacagcctg aattcaggtc atagtaaatc agccagtgtc gtggtttcca gtttttgagc
      181 gatgaagtcg cgtacttcgc gcagcacgtc aatcatcttc tgttcttttt cgagcggcgt
      241 cgccttgtaa aaatatttgc tgaccgattc cgttggggcc agcgccccat caaagagccg
      301 aatcacctgg gccagtgaaa tttcactggg atttcgggca agtcggtatc cgccgtgctg
      361 gcccttgatg ctggaaacat acccggagcg ctgcatcgcc gacataatct gttcgagata
      421 tttgagtggg attttctggg cttcggcaat ccgcgccgat gcaatcgggc cgtcggtttg
      481 attccggccc aggtaaatca aagccagcaa agcatattca ctgcgggtgg tgagtttcat
      541 agaaagtttt cctagtaaac cgataggaat tgtattatat tggccgccca taagcagtca
      601 gccatcaaaa tccttgtttt ccacaataca catccacgta cctcgctggc aatcagggat
      661 acttccaact tcacgcacgc tgttttgaac tcgattcagc cacaacctga acctttgagg
      721 gtagacctat gtcttcaacc gtttctgttg atgtacaaac cgatgtcttg accatcactg
      781 actttgtcaa tcgcctgacc cggcttgacc agaacgaacg aacacccgac gtgattgcag
      841 cgctgttcca acaagttcaa cgttttgacc cggaagttga gcttcacgtt acgttttcca
      901 gacaaaagta cactcgaaac ctgttttttc gaaatgaagc atttgaactc ctgattatgt
      961 gctgggaacc cggccagggc acactgattc acgaccatga tggttcattt acagtcgata
     1021 aaatttattc tggtagtctg caatgtattg attttcaacg aactcatcta aattcatcta
     1081 cgcttgaaga aactcgccgg acccaactca ttcctggcaa tgtcatgata tcgagttccg
     1141 aggatatcca ccgcgttgtg aatatttcag accagacacc agcggtttca atccatctgt
     1201 atgcgccacc actcaagcga atgaattgtt ttgacctgga aaccgggaca acctgggtgg
     1261 tgattccttg atcagggttc aggattcgga acaacaatca atcggctatt ttcgtcaagt
     1321 cactcatgat gataaccagg aaggtttttg accttaaatg gctgtgtcca gatgtcatag
     1381 ggtttttctg aaaaccatcc agtaatacaa atcctgagtt ttacatttcc tccgtttgga
     1441 aactcatatg ggctcaaata aattttttgg cttgagttcc ctttcaaccc agccgttccc
     1501 aaccctgtgc cgcaaaacgc caggacactc gttcgccagt acccgccacg gttcgactga
     1561 tatttgtaaa ttggcgtttc tgg
//
LOCUS       DAOQDO010001759         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87794_length_1583_cov_2.613220, whole genome shotgun sequence.
ACCESSION   DAOQDO010001759 DAOQDO010000000
VERSION     DAOQDO010001759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87794_length_1583_cov_2.613220"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKZ53_22435"
     CDS             complement(<1..497)
                     /locus_tag="PKZ53_22435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43260.1"
                     /translation="MRNILQDVKYSFRLLIKKPAFSILIILTLALGIGANTALFSVLY
                     GIVFKPLAINEPARVVTIYQYYHRMHMRGANSPPNFVDIRNQCQSFESVAATVPWNAN
                     LVGEGEPSRVFGLQVSSNFFQTIGMQPVLGRAFVPEEEQAGAPLVADGRPDVVFNESA
                     RWRDRR"
     gene            868..1533
                     /gene="pnuC"
                     /locus_tag="PKZ53_22440"
     CDS             868..1533
                     /gene="pnuC"
                     /locus_tag="PKZ53_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNJ43261.1"
                     /translation="MKHLDYWMVFGLGIAMTLGSAFHWLPYSLTETLGFVTGAACVYL
                     VVKEHIWNFPIGIANNLFFLVLFGQARLYGDAGLQVVFMLLGIQGWYVWLYGGKNRTH
                     RQIAHATPPMLGLVTGFVLFGTYGLTQILHRVSGAAPLLDAFTTVLSLAAQYLLNCKL
                     IENWYVWILANIIYIYLYLHRGLQLTAILYFVFLCLCIAGLINWTQTFKKQSGTLTCQ
                     SVD"
BASE COUNT          375 a          396 c          396 g          416 t
ORIGIN      
        1 cgtcgatcac gccaacgagc ggattcattg aatacaacgt ccggacggcc atcggccacc
       61 agtggagcgc cagcctgttc ttcttccggc acaaaggcac gtccgagcac tggttgcatc
      121 ccaatggttt gaaaaaagtt agatgaaact tgcaggccaa acacacggga cggctcacct
      181 tcaccaacca gattggcatt ccagggaacg gtggcggcaa ccgattcaaa ggactgacat
      241 tggttccgga tatccacaaa attcggcggc gaattggccc cgcgcatgtg catgcggtga
      301 taatactgat agatcgtgac cacccgggct ggttcgttga tggccagcgg tttaaacaca
      361 atgccataca agacactgaa caaggcggta ttggccccaa taccaagtgc cagcgtcaga
      421 ataatcaaaa ttgaaaacgc gggtttcttg atcagcagtc ggaagctgta cttaacgtcc
      481 tggagaatat ttcgcatagg attttggtgg attcagacat gagattcccc acacaagtgg
      541 aaaagcgaac cggaagacag atttgaggag gtttgcaaaa tgcagcgcaa aggccgtgcc
      601 atagccaaat gttgcctcaa ccactgagtt tgcttttatt tgtaaggttt tgtctgacag
      661 gctaaactat gttttttgct ccagttctcg tcccttcact ctacctgcct gccgtttgtg
      721 cctgatgatt caaatcggat ttccatccag attggtacct ggggccaggc actcttgttc
      781 ggtaacgaac actgactttt tcaaaaatga acagcccgaa gagagagttg gctgtcaatt
      841 caacccttcc ggaacgagtg agccatcatg aagcaccttg attactggat ggtttttggg
      901 cttggaatcg ccatgacgct cggatctgcg tttcactggc tgccttatag tctgaccgaa
      961 accctggggt ttgtgactgg ggccgcctgt gtttatctgg tcgtcaaaga acacatctgg
     1021 aattttccca tcggtattgc caataacctt ttctttctgg tgctctttgg gcaggcacgg
     1081 ctctatggag atgccgggct tcaggttgta tttatgctcc tggggatcca aggctggtat
     1141 gtgtggctct atggcgggaa aaatcgcacc caccgtcaga ttgcccatgc cacgccgccc
     1201 atgctggggc tggtgaccgg gtttgtcctg tttggcacct atggattgac ccaaatcttg
     1261 caccgggtga gtggggcggc gccgcttctg gatgccttta ccaccgtgct gagcctggcc
     1321 gcccagtatt tgctcaactg taaattgatt gaaaactggt atgtctggat cctggcaaat
     1381 atcatttata tctacctgta cctccatcgt gggttgcaat taaccgccat tttgtacttt
     1441 gttttcctgt gcctgtgtat tgccggtctg atcaactgga cccaaacctt taaaaaacaa
     1501 tccggaacac tcacatgcca atccgtggat taggttctgt tgacatttat tgtaaaatgg
     1561 gagcatgaca cctctcaaac tca
//
LOCUS       DAOQDO010001760         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87801_length_1583_cov_2.504581, whole genome shotgun sequence.
ACCESSION   DAOQDO010001760 DAOQDO010000000
VERSION     DAOQDO010001760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87801_length_1583_cov_2.504581"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1067
                     /locus_tag="PKZ53_22445"
     CDS             126..1067
                     /locus_tag="PKZ53_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNJ43262.1"
                     /translation="MKQRLTENKLILIKRRTRLDEMIVRYNTVNQAKFYIEHQNADFS
                     DYQAEHDQYNQALRETENWLGEFGRVQVVDRAFLPNFIFGKDDTIVVLGQDGLVANTV
                     KYAPALPIIGVNPDPKRWDGVLLPFTVQDLPKIVPEVFSQRRQFQEVSMAKAQLNNGQ
                     TLYAVNDFFVGVKSHTSARYTLQIGNQKEQQSSSGVIISTGLGSTGWFTSLMRGALEV
                     ASRISGQTLSHQYFQKLSWDADMLYYTVREPFPSKTSATNLVFGAITPATPLKLISQM
                     SEAGVIFSDGIERDFLEFNSGTQATITLAEEKGHLIV"
     gene            1157..>1583
                     /locus_tag="PKZ53_22450"
     CDS             1157..>1583
                     /locus_tag="PKZ53_22450"
                     /inference="COORDINATES: protein motif:HMM:NF026931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm42 family immunity protein"
                     /protein_id="HNJ43263.1"
                     /translation="MFFGSQNTFGIECEYDPSFFHPTCFFGHICFWIGNHPVGDFQEF
                     VMLDIPAIHLKDTLRFEGQRRDPAIDNQQAQVILEIVSKALWGNDDFENDDFQQLKNR
                     FQRFSICPNGGEAFDGIQCVLVDEDDSERMIWKWRGSPPE"
BASE COUNT          407 a          374 c          377 g          425 t
ORIGIN      
        1 gggttccggg ttccgggttc cgggttccgg gttccgggtt ccgggttttc gaatgtatgt
       61 ccttttcgtc cttttcgtcc tttttgtttg ttcctgaacc ctgaaccctg aaccataaac
      121 cctttatgaa acagcgtctt accgaaaata aactcatcct gatcaagcgg cggacccgcc
      181 ttgatgaaat gatcgtgcgg tataacacgg tcaatcaggc aaagttctat attgagcacc
      241 aaaacgctga tttttctgac tatcaggctg agcacgatca atacaatcag gcgctccgcg
      301 aaactgaaaa ctggttgggc gaatttgggc gggttcaggt ggtggaccgg gcatttttgc
      361 caaattttat tttcggcaag gacgacacaa ttgtggtgct tgggcaagac ggactcgtgg
      421 ccaatacggt caaatatgcg ccagccttgc cgatcatcgg cgtcaacccg gacccaaaac
      481 gctgggatgg cgtgctgctg ccctttactg ttcaggattt accaaaaatt gtcccggagg
      541 ttttcagtca gcgtcgccaa tttcaggaag tctcgatggc caaagctcaa ctcaacaacg
      601 gccagacgct ctatgccgtc aatgattttt tcgttggggt taaatcgcac acgtcagcac
      661 gatacaccct ccaaatcgga aatcaaaaag aacaacaatc atcaagtggc gtcatcattt
      721 cgaccgggct tggttccact ggctggttta ccagcctgat gcgtggtgcg ctcgaagttg
      781 cttcgcggat ttcaggccag acgctgtcac atcagtattt tcagaaacta agctgggacg
      841 ccgatatgct gtactacact gttcgcgagc cattccccag caagacttct gcgaccaacc
      901 ttgtctttgg tgccattaca ccggccacac cactcaaact catttcacaa atgtcggagg
      961 ccggcgtgat ttttagtgat ggtattgaac gcgatttcct ggaattcaat tctggaaccc
     1021 aggccaccat cacactagct gaagaaaaag ggcatttgat tgtgtgaaga accctggcct
     1081 tgagttcttc aacggcattt taagttggag gtaccaactg aatttctgaa gcactttgag
     1141 aggagtccaa atgaaaatgt tctttggatc ccaaaacaca tttggaatcg agtgtgagta
     1201 cgacccctca ttttttcatc caacctgctt tttcggtcat atctgttttt ggattggaaa
     1261 tcacccggtt ggtgatttcc aggaatttgt catgctggat attccggcaa ttcacttaaa
     1321 agatacattg cgctttgaag gccaacgaag agaccctgcg attgataatc agcaggcaca
     1381 agtcatttta gagattgtct ccaaagctct ctggggaaat gacgattttg aaaatgacga
     1441 ttttcaacag cttaaaaatc gcttccaacg gttttcaatc tgcccgaatg gaggtgaagc
     1501 ttttgacgga atccaatgtg tattggtaga tgaagatgac agtgaacgaa tgatatggaa
     1561 atggagagga tcgccacccg aaa
//
LOCUS       DAOQDO010001761         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87838_length_1583_cov_1.843586, whole genome shotgun sequence.
ACCESSION   DAOQDO010001761 DAOQDO010000000
VERSION     DAOQDO010001761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87838_length_1583_cov_1.843586"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..919)
                     /locus_tag="PKZ53_22455"
     CDS             complement(221..919)
                     /locus_tag="PKZ53_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43264.1"
                     /translation="MLKKSQEADDSVKQPVVDDSSEVSFEEGLVVESAEIDPLPIVRQ
                     GNIVRLSTDALSVEARAAIHAELAEESGQTFFIGKIEHGYSLQSARHPDQCPRCQSPT
                     KTHMASLIYATNRGPRVLTTRGVHFCTACPTVIVDEDMVERSIAEEFEYRGLLGLDFD
                     GKAEPCFFQYWNEQEMIILVDQETGFMELVPKSELKLSKPAIGKGLTRQVSKKKSSRS
                     AMAKKSRKQNRKKK"
     gene            1048..1521
                     /locus_tag="PKZ53_22460"
     CDS             1048..1521
                     /locus_tag="PKZ53_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HNJ43265.1"
                     /translation="MAFILKPPHPLDLAPDRPGLFLAGSIEMGRAVHWQALFEQELSD
                     LDVTILNPRRDDWDASWEQSLRNPNFRGQVEWELDAQEKADAIAMYFAPETHAPITLL
                     ELGLFASSQKLIVCCPEGFWRKGNVEVVCLRYGVPTVETLPELVAATRRRMLALL"
BASE COUNT          341 a          431 c          379 g          432 t
ORIGIN      
        1 gccgcttgtg tggttggccc tgaaggaaag ttttgtttcc ccaaacttcc ccctggtcag
       61 tactttcttg tagccggatg cctctcaagt gaggcgggga taaacacaac acaaattgtc
      121 gtcacaattg atccgaaagc gaagaaagac cacccattgg agctcagtct gtcgccaggg
      181 acttgaagaa atcaggcata cctctttcta ttccccaacc ttacttcttc ttccggtttt
      241 gcttgcggga ttttttggcc atggcgcttc gacttgattt ctttttcgac acttgacggg
      301 ttagcccttt ccctatcgcg ggtttggaca gtttcaactc gcttttcggc accagttcca
      361 tgaacccggt ttcctggtcc accaggatga tcatttcctg ctcattccaa tactgaaaga
      421 aacagggttc agctttccca tcaaaatcga gcccaaggag cccccggtat tcaaattcct
      481 cggcaatact tcgctcgacc atgtcttcat ccacaatcac agttgggcac gccgtacaaa
      541 aatgaacgcc tctggtcgtc aaaactctag ggccccggtt ggttgcatag atcaggctcg
      601 ccatgtgggt ttttgttggt gactggcatc tggggcattg gtcgggatgg cgtgccgact
      661 ggagagaata cccatgctca atcttgccaa taaaaaatgt ctgcccgctt tcttcagcca
      721 gttcagcgtg gatcgctgcc cgagcctcga cgctcagggc atcggtgctg agtcgaacga
      781 tattcccctg gcgaacaatc ggaagagggt caatttcagc ggattcaacc accaaccctt
      841 cctcaaaaga cacctctgat gagtcgtcca cgacaggttg tttgactgaa tcatccgctt
      901 cctgcgattt tttcaacata cattcctcac tttcctgaat taaatttttc aagcccaacg
      961 agttttgcga taatcccaac cgttaccatc aaccagaaat tgtttcaacc atattccttc
     1021 ccttctccaa ctcaaggaca tccgtttatg gctttcattt tgaaacctcc gcatccgctt
     1081 gaccttgccc cagatcggcc aggtctcttt ctggctggtt cgattgaaat gggccgggcg
     1141 gtacactggc aggcactgtt cgaacaagaa ctgagcgacc tggatgtgac catcctcaat
     1201 ccacgccgtg atgattggga cgccagttgg gaacagagtc tccgcaatcc aaactttcgc
     1261 ggtcaggttg aatgggaact cgacgcccag gagaaagcag atgccatcgc aatgtatttt
     1321 gctccggaaa cgcatgctcc gatcacattg ctcgaacttg gactctttgc cagcagtcaa
     1381 aaattgattg tgtgttgtcc cgaagggttt tggcgcaagg gcaatgtgga ggtggtgtgt
     1441 ctgcgctatg gcgtaccaac ggtcgagacg cttccggagc tggtcgccgc cacccgccgc
     1501 cgaatgctgg ccctgctgta ggaattggta cctggttctt ggttcttggt tcttggttct
     1561 tggttcttgg ttcttggttc ttg
//
LOCUS       DAOQDO010001762         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87901_length_1582_cov_2.582187, whole genome shotgun sequence.
ACCESSION   DAOQDO010001762 DAOQDO010000000
VERSION     DAOQDO010001762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87901_length_1582_cov_2.582187"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..>1582
                     /locus_tag="PKZ53_22465"
     CDS             535..>1582
                     /locus_tag="PKZ53_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43266.1"
                     /translation="MKKSLFCLVSLFLVQLVVGGNLVLAATPSGSTTQFLVPQQVANA
                     STTGGSSGTATDAAASQDQEYNDMLAATDEKDAELRYKKIQEFIGKYPQTQYKAFVYA
                     RLNGARGVLINKSLGKGNMADAHKYGRELFAEPKENKEGLDDFPYYWLLGRASGLIAI
                     QSNDNGYLKVGAEYSQKALDLIAQGSKIDNKSIKWEEKKNSIAADLHRTIAMMHYIDA
                     KYDDATREFEEAIKVDCDAYGLYYRGRIKFIDFTAAAKAYNDIPDKTTPDTATALATA
                     KDKARVASEYLAKAIAFIEKRSDAATFKSFSDDARKLLDPVFNFAYEDKINEQPAPPP
                     APEPAGPAPVYAGFW"
BASE COUNT          428 a          371 c          399 g          384 t
ORIGIN      
        1 ttgtatagtt gtcttctttg ctgtagggaa ttcgcttcaa aggcatgccg tgggtgctcc
       61 tcttggtaga aagtcgggat gatgtgagga agggattttt ttgagaatcg gtatgcgcgt
      121 tatacaccaa gccgctcctg gttgtccaaa tgcatttctg aaattgccgg tcaccgcact
      181 tgattgaatc agaggaagaa gccctggtgg tttgtcggtc aactggattt cagattggct
      241 tgactgaagg ctggctgccg gcaacaacac cagtcaggtc attgaaataa ggtccgaaca
      301 gtccgaaaat cggaaggaag tccgaaagcg ttgacaaacc catggccgcg tgtcagcatc
      361 cgaaattcgt gaaaaacact gtgcacaacc acattccagg cttttcgcgt gttcgacaaa
      421 ctttgctttt acaggcatcg ctggtaaggt aaaaatcatc actcaattcg cctgtccaac
      481 atttcaccgt cataaaaatt tcatatttcg ccttaggagg agcttaaccc ccttatgaaa
      541 aagtctctgt tttgccttgt cagtttattt ctggtccaac tggtggttgg cggaaatctt
      601 gtccttgccg ccaccccatc agggagcaca actcagtttt tggtcccgca gcaggtggca
      661 aatgccagta cgactggtgg ttccagtggt acggcaaccg atgccgccgc aagtcaggat
      721 caagaatata atgacatgct ggccgcaact gatgagaaag atgctgaatt acgctacaaa
      781 aaaattcagg aatttattgg gaaatacccc cagactcagt ataaagcctt tgtttatgct
      841 cgattgaatg gtgcgcgcgg cgtattgatc aacaaaagcc tggggaaagg gaacatggct
      901 gatgcccaca aatatggaag ggaactgttt gccgagccaa aggaaaacaa agaaggcctg
      961 gatgactttc cttattactg gctgcttgga cgtgcttccg ggctgattgc gattcaatcc
     1021 aatgacaatg gctatctgaa ggttggggct gagtattcgc aaaaagccct ggacctgatt
     1081 gctcagggaa gcaagattga taacaaaagc attaagtggg aagaaaagaa gaattcaatt
     1141 gccgcagatc tgcatcgcac gattgccatg atgcattaca tagatgccaa atacgacgac
     1201 gcgacccgag agtttgaaga agccatcaaa gttgattgtg atgcctatgg gctctattat
     1261 cgtggccgga tcaaatttat tgacttcact gccgctgcca aagcgtataa cgacattccg
     1321 gataaaacca cccctgacac ggccacagcc ttagccaccg ccaaggacaa agccagggtt
     1381 gccagcgaat atctggccaa agcgattgct tttatcgaga agcggtcaga tgcggcgacg
     1441 tttaagtcat tttcggatga tgcccggaaa ttgttagatc cagtctttaa ttttgcctac
     1501 gaagacaaaa tcaacgagca gcctgcaccg ccaccggcgc cggaaccggc cggccccgcg
     1561 cccgtgtacg ccggtttctg ga
//
LOCUS       DAOQDO010001763         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87929_length_1582_cov_2.060904, whole genome shotgun sequence.
ACCESSION   DAOQDO010001763 DAOQDO010000000
VERSION     DAOQDO010001763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87929_length_1582_cov_2.060904"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKZ53_22470"
     CDS             <1..305
                     /locus_tag="PKZ53_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43267.1"
                     /translation="GVLINLPVILVAQEQLGIETRFRPMDRSELYVADEAFFCGTGAQ
                     IAAIATIDHRPVGNGKIGPITEKLTKLYNKMVHGELPQYNHWLTPCFGHKASVVAG"
     gene            321..1127
                     /gene="mqnB"
                     /locus_tag="PKZ53_22475"
     CDS             321..1127
                     /gene="mqnB"
                     /locus_tag="PKZ53_22475"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNJ43268.1"
                     /translation="MGTRKPNQGTRGYSLSQDPEPGAGNPEPVLLLAAVTKESRGVCA
                     QIQNQQTVNFPAGVSAIFGTLIGQPVALVLTGVGPVRAAAVLAVVLAQTPFSCVLQFG
                     VGGAYPHSGLNIGDLVLAESECDPQTGLESPEGFRDLRGLGFPITDHFPNKQLLENNW
                     IEFIAGALPGVMRGGVATVATCSATDALADRMEHLTGAKVEAMEGFPAAWVSAQFGIP
                     FAELRAISNTTGDRARQQWDLSRGCDVATEAVLTIFREMTICQGDQISPS"
     gene            1236..>1582
                     /locus_tag="PKZ53_22480"
     CDS             1236..>1582
                     /locus_tag="PKZ53_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNJ43269.1"
                     /translation="MKLTLGYSPCPNDTYIFGALALGLVQLPGLDFEIRLEDVETLNQ
                     LAKAGTLDVTKISYAALADGLLEEYQLLASGGALGRGCGPLIISRTPLNSNDLTSARL
                     VTPGKLTTAHLLTR"
BASE COUNT          350 a          426 c          419 g          387 t
ORIGIN      
        1 aaggcgtact gatcaatctg cccgttatac ttgtggccca ggagcaactc gggattgaaa
       61 cccgttttcg tccaatggat cggtctgaac tctatgtcgc tgatgaagcc tttttctgcg
      121 gtacgggtgc tcaaatcgca gccattgcaa cgattgacca ccgtccagtt ggcaatggca
      181 aaattggccc gatcactgag aaactgacca aactctataa caaaatggtg catggtgaat
      241 tgccgcaata caaccactgg ctgaccccgt gttttggcca taaagcctcc gtcgtcgccg
      301 ggtaagattt cgagggtaga atgggaaccc ggaaaccaaa tcagggaaca cgaggttaca
      361 gtttgtctca agacccggaa cccggagccg ggaacccgga acccgtttta ctcctagctg
      421 ccgtcaccaa ggaatccagg ggcgtttgcg cacagatcca aaaccagcag accgtaaatt
      481 tcccggcagg tgtttcagcc atttttggaa cgctgatcgg gcaacctgtg gcgctcgtgc
      541 tgaccggtgt cggtccagtt cgagcggcag cggttctggc agtggttctg gcgcaaacgc
      601 ccttttcgtg tgttttgcag tttggggttg gtggtgcgta cccacattca ggtttaaaca
      661 ttggcgatct ggtacttgct gagagtgaat gcgatccgca aaccgggctt gaatcacccg
      721 aggggtttcg cgacctgcgg gggcttggat tccccatcac tgatcatttt ccaaataaac
      781 aattgcttga aaacaactgg attgagttta ttgccggggc actgcccggt gttatgcgcg
      841 gcggcgtggc caccgttgcc acctgctcgg ccacagatgc actggctgac cgaatggaac
      901 acctgaccgg tgccaaagtt gaagccatgg aaggtttccc cgccgcctgg gtgtcggctc
      961 aatttggaat tccgtttgct gaactccgtg ctattagcaa cacgactggc gaccgggccc
     1021 gtcaacagtg ggatctctcc aggggctgtg acgtcgccac cgaagctgtc cttacaatct
     1081 ttcgcgaaat gaccatatgc cagggtgacc agatttcccc atcctaaaat tgcttgcctc
     1141 ttgatcccca tctctgcttt gctctgatca cacattttga aacttataga aagccttact
     1201 tcatttttga agctgaggtc aggtgtttat tgcccatgaa actgacattg ggatattcgc
     1261 cctgtccgaa tgacacctac atttttggcg cgctggcgtt gggtctggtt caacttccgg
     1321 gtctggactt cgagattcgg cttgaagatg tcgaaacgct caatcaactg gccaaagctg
     1381 gaaccctgga tgtgaccaag atttcctacg cggcactcgc tgatggattg ctggaggaat
     1441 accagcttct ggccagcgga ggagcacttg gacgtgggtg cggcccgctg attatttcac
     1501 gcactccatt gaattcgaat gacttaacct cagcccggct ggtaactccc ggaaaactga
     1561 ccaccgccca tttgctaacc cg
//
LOCUS       DAOQDO010001764         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_87990_length_1581_cov_2.973132, whole genome shotgun sequence.
ACCESSION   DAOQDO010001764 DAOQDO010000000
VERSION     DAOQDO010001764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87990_length_1581_cov_2.973132"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..735
                     /locus_tag="PKZ53_22485"
     CDS             151..735
                     /locus_tag="PKZ53_22485"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ43270.1"
                     /translation="MNILSQSKPQTESRTDEELVTNVLAGKGEVFSLLYERYYQRVFR
                     LAAGMTGATVAEDLTQEIFFRVFQKLNQFAGQSRFSTWLYRLAVNHCLNARESARPQT
                     QVDTELELLELPSTHTHLEKAVIEKQLQAHVQRALLTLKPEIRMIIILKDIEGLNYDE
                     IAERMGCSPGTVASRLNRGRKLLAEKLAPLRGKI"
     gene            829..>1581
                     /locus_tag="PKZ53_22490"
     CDS             829..>1581
                     /locus_tag="PKZ53_22490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNJ43271.1"
                     /translation="MNARHFFQHLIHSVLHRGYRRQFSPWLDDELNLPARTALEAHLQ
                     HCELCRKELETLAFASRTVSQIGIPLPENDLVPVWRPVPRSDSNPIRFGWWKVATVGV
                     AMAGLIVAWWSVGHFLHHPGSSPAVFWEVVNLEGSPAIDAHQIAQTAQFGPGQWLETD
                     ARSRAKITVGEIGSVEIDPNTRIKLVQAGQAEHRLALDEGRMHARIYAPPRVFFVDTP
                     SAVAIDLGCAYTLEVNKDGDSTLQVTSGWVALM"
BASE COUNT          355 a          431 c          422 g          373 t
ORIGIN      
        1 gttccgggtt ccgggttccg ggttccgggt tccgggttcc gggttccggg tttcgaaagt
       61 tgatcctttc cgctgattct ttcctggtcg tccctttttc atgaaaagat tttggaagac
      121 gattgaactc gcgcctgtca aaatggtcga gtgaacattt taagccaatc aaaaccgcaa
      181 actgaatcgc gaactgacga agaactggtc accaacgttc tggccggtaa gggcgaagtc
      241 ttttctttgc tctatgaacg ctattaccag cgcgtcttcc gattggcagc ggggatgact
      301 ggtgccacgg tggctgaaga cctgacgcag gagatttttt ttcgcgtgtt tcaaaagctc
      361 aatcagtttg ccggacaatc gcgattttcg acgtggctct accgcctggc ggtcaaccat
      421 tgcctgaatg cccgcgaatc tgcccgtccg caaacccagg ttgatactga acttgaactc
      481 ctggagttac cttcaacgca cacccacctt gaaaaagcag tcattgaaaa acagcttcaa
      541 gcccacgttc aacgggcttt gctgacgctc aagcctgaaa ttcgcatgat tatcatttta
      601 aaagacatcg aaggtctgaa ctatgacgaa attgccgaac ggatgggatg ttcgcccgga
      661 acagtggctt cacgcctcaa tcgaggccgc aaattactgg ctgagaaatt agccccgttg
      721 cggggaaaaa tctgaagtgg ggctaagggc taagggctaa gggctgaaga aaacgggtaa
      781 ttcagcccca agccccaagc cccaagcccc aggccctaag ccctaatcat gaatgcacgt
      841 cacttttttc aacacctcat ccactcagtg ctccaccggg gctaccggcg gcaattttcg
      901 ccgtggctgg atgatgagtt gaaccttcca gcccggaccg cgctggaagc ccatttacaa
      961 cactgcgaat tgtgccgcaa agagcttgag actctggcgt ttgcttcacg aacggtttct
     1021 cagattggga tccccctgcc ggaaaacgac ctggttccag tctggcgacc tgtcccccgt
     1081 tcagattcaa atcccattcg atttgggtgg tggaaagtcg caaccgttgg ggtggcgatg
     1141 gctggtttaa ttgtggcctg gtggagcgtc gggcactttt tgcatcatcc agggtcttca
     1201 ccagcggttt tttgggaggt tgtcaacctc gaaggtagcc cggcgattga cgcacatcag
     1261 attgcccaaa cggcccagtt tggccctgga caatggcttg aaactgatgc cagatcccgc
     1321 gccaaaatca ccgtcggaga aatcggctcg gtcgaaattg acccaaacac ccgcatcaag
     1381 ctggttcagg ctggtcaggc cgaacatcgc cttgcccttg acgaaggccg catgcacgcc
     1441 cggatttatg ctccgccacg ggtgtttttc gtggataccc cgtcggcggt ggcgattgat
     1501 ttaggatgtg cctacaccct cgaagtcaac aaagacggcg acagcacctt gcaggtcacg
     1561 tccgggtggg tcgctttgat g
//
LOCUS       DAOQDO010001765         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_88024_length_1581_cov_2.306684, whole genome shotgun sequence.
ACCESSION   DAOQDO010001765 DAOQDO010000000
VERSION     DAOQDO010001765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88024_length_1581_cov_2.306684"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1581)
                     /locus_tag="PKZ53_22495"
     CDS             complement(<1..>1581)
                     /locus_tag="PKZ53_22495"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNJ43272.1"
                     /translation="GSFEPVDVNKIVRAVSRCCHGLYYVDALRVATKTISGLYDGATT
                     KELDQLSIQTAASLIVEEPEYSRLAARLLGTYIDKEVTNQEIHSFSQSITAGHKLGLF
                     SDRLAEFVSANSRKLNAAITGQRTELFEFFGIRTLYDRYLVKHPTKRDVIETPQYFFM
                     RVACGLAETVKDVIDLYHLFSSLSYVPSSPTLFNSGTKHEQLSSCFLLDSPEDDLEAI
                     YKKYTDVALLSKFSGGIGIAYSRVRSQGSLIRGTNGHSQGIVPWLKTLDASVAAVNQC
                     FAPETLVYTAHGVKEIKDIKVGDLVLGQRGKYREVLKHMTYFQRDPMVEIKVKHSISP
                     IKVTAGHPMWAIQGIPMEQSLERTMSQLEREKFVPAWVEAGQLRKGDYVAQVIPTEVV
                     PVPGFTEDDARMYGILLSDGHLAKDNDEWGVSGNPLTDTHIEFVRQYLNNANIHYWQT
                     SRGENYLQIRWAAGRGVVREATTGRFLSSGASTLPFTREDLYDENGQKHIARRFSHLP
                     PQQTRALIQGLIETDGGI"
BASE COUNT          376 a          395 c          412 g          398 t
ORIGIN      
        1 cttatgccac catcagtttc aatcagaccc tgaataagtg cgcgggtttg ttgcggaggc
       61 aggtgcgaaa accggcgggc aatatgtttt tggccgttct cgtcatacag gtcttctcgc
      121 gtgaagggca atgttgacgc tccgctgctg agaaatcgtc cagtggttgc ttcccgaacg
      181 accccacgtc ctgccgccca ccgaatttgc aggtaatttt caccccgact cgtttgccag
      241 tagtgaatat tggcattgtt gaggtattgg cgaacaaact cgatgtgtgt gtcagttagt
      301 gggtttcctg aaactcccca ttcatcattg tcttttgcaa gatgaccatc actgagcaag
      361 attccgtaca tccgggcatc gtcttcagtg aatcctggaa ctggaaccac ctcagttgga
      421 atgacttgtg ccacgtagtc gccttttcgg agttgaccgg cttcaaccca ggctggaacg
      481 aatttttcac gctccaactg tgacatcgtg cgctcaaggg attgctccat cggaatcccc
      541 tgaattgccc acattgggtg accagccgtg actttgatgg gagaaatcga gtgtttgact
      601 ttgatttcaa ccattgggtc gcgttggaaa taggtcatat gcttgaggac ttcgcggtat
      661 tttccacgct ggccaagcac cagatcgcca actttaatgt ctttgatttc cttcacccca
      721 tgtgcggtat agaccagcgt ctctggagca aagcactggt tcacggctgc caccgaagca
      781 tctaacgtct tgagccacgg gacgataccc tgggagtggc cattcgttcc gcgaatcaat
      841 gacccctggc tgcgcactcg cgaataagca atgccgatac cgcctgagaa tttcgacagg
      901 agcgcaacgt cggtgtattt cttataaatt gcttcgagat cgtcttcggg ggaatcaagc
      961 agaaagcacg atgaaagctg ctcgtgtttg gtgccggaat taaacagcgt cggagaactt
     1021 gggacatagg acagtgatga aaaaagatgg tacaggtcaa tcacatcctt gacggtttct
     1081 gccagtccgc aggccacacg cataaagaaa tactgtggcg tttcaatcac atcacgtttg
     1141 gtcggatgct tgaccagata gcggtcatag agggtgcgaa tcccaaaaaa ttcaaataat
     1201 tcagttcgtt gcccggtaat cgcggcattg agcttgcggc tattggctga gacaaattca
     1261 gccagccggt ctgaaaagag cccaagcttg tgaccagccg tgatggattg tgaaaacgaa
     1321 tgaatttcct gattcgtgac ttctttatca atataggtgc ccagcaaccg ggcggcgagg
     1381 cgtgagtatt caggttcttc gacaatcagc gacgcggcgg tttggatcga aagttgatca
     1441 agttccttcg tggttgcgcc gtcatagaga ccgctgatgg ttttggtcgc aacccgcagg
     1501 gcgtcaacat aatagagtcc atggcagcag cggctcaccg cgcgcacaat tttattgacg
     1561 tcaaccggct caaacgatcc a
//
LOCUS       DAOQDO010001766         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_88058_length_1581_cov_1.841415, whole genome shotgun sequence.
ACCESSION   DAOQDO010001766 DAOQDO010000000
VERSION     DAOQDO010001766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88058_length_1581_cov_1.841415"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1422)
                     /locus_tag="PKZ53_22500"
     CDS             complement(13..1422)
                     /locus_tag="PKZ53_22500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ43273.1"
                     /translation="MNCHRHQSAVSSRRSRGFSLLELLIVLALFTVVMGAVFVTLDYA
                     KKTSFTNNQLVDTQQNIRASAKIMADDVITLGQDFMGQISLDSVYVRRGFLAANSFPD
                     TDVTTGAAYDELFAAQWANNVNSTQAVLGVDTNGNPSTNIRVAPGSGPAILGDGSAGY
                     IPAYGTGTDQLLFVQADSITLRFADDFGLVQPDDNTDIELGEARYFAQANFSGSDLIL
                     LPLTGSSPASNFDNATSAALVDQSDRLMARRLVPMVDVIIVRSTTGGAQFMGLVTAVN
                     QTSGAITLSYANDPIQLTPDWTYPLTEASGGRTGTHPRLCGQGDVVSIRRGRILRYFV
                     GSFINTPAGAPTDFCALYRRDGARIDPVAFSIENMHCTLLLADEEVLNAGRGQFYTMP
                     GATLTDLNTAPPQPPAAGRTRRWSRTAIRSIRVHLFGRSDEYDPSLNRQVGAATRKGD
                     YLRINQEFTISLRNSVYNR"
BASE COUNT          422 a          392 c          386 g          381 t
ORIGIN      
        1 ccagcttgag ctttatcggt tgtaaaccga gtttcgaaga gaaatggtaa actcctgatt
       61 gatccgtaag taatcacctt ttcgtgtcgc agctccgacc tgacgattga gtgagggatc
      121 atactcatca gatcgtccaa aaagatgaac tcgaatggat cggatggcgg ttcgactcca
      181 acgccgggtt cgccctgcgg ctggaggttg aggtggagca gtatttaaat cagtcagtgt
      241 tgctcctggc atcgtataaa attgtccacg cccagcatta agaacttctt catcagccag
      301 caacaaggtg cagtgcatgt tttcgatact aaatgctact ggatcaatcc gggcaccatc
      361 gcggcgatac agggcgcaaa aatcggtggg ggcgccggct ggagtattga taaacgaacc
      421 aacaaaataa cgcagtatcc gaccacggcg gatacttacc acatcgcctt gtccgcacag
      481 ccgagggtgc gtgccagtac gaccacctga ggcttcagtc aaggggtatg tccaatcagg
      541 ggtcagttga attgggtcat tggcatatga caatgtgatg gcacctgaag tttggttgac
      601 ggctgtaacc aatcccataa attgcgcccc acccgtcgtg ctgcggacaa ttattacgtc
      661 aaccattggc actaatcgcc gggccattaa ccgatcactt tgatcaacca gggccgcaga
      721 cgtcgcattg tcaaaatttg aggctgggct tgaacctgta agcggtaata aaatcagatc
      781 actaccacta aagtttgcct gggcaaagta tctggcctca ccaagttcaa tatcagtatt
      841 gtcatcaggc tggaccaacc cgaaatcatc tgcaaatcgc aacgtaatcg aatccgcctg
      901 gacaaacaat aactgatcag tccctgtacc ataggccgga atatagcctg cggacccatc
      961 tcccaggata gcggggcctg aaccaggcgc aacacggatg ttcgttgaag ggttgccgtt
     1021 ggtatcaacg cctaatacag cctgtgttga gttgacgtta ttggcccatt gggcagcaaa
     1081 tagctcgtca tacgcggcac ctgttgtcac atcagtatct gggaaagaat tggctgccag
     1141 aaatcctcgc cggacataca ctgagtcaag tgaaatctgc cccataaaat cctgacccag
     1201 cgtaatgaca tcatctgcca taatcttggc tgatgcccgg atattctgct gggtatcaac
     1261 cagttgatta ttggtaaacg atgtcttttt ggcatagtcg agggtcacaa agacagcccc
     1321 catcaccaca gtaaaaagag caaggacaat caggagttca agcagcgaaa acccgcgact
     1381 ccggcgagat gaaacagcag attgatggcg gtggcaattc attgtttttt cgactccggg
     1441 gttgaattct tcattctcaa ttcttcattc cacttagatt tttccaggaa caatccattc
     1501 atctttatga agtcgccaga ctgaagttcg tccagtggcg cctaaaacag tgatggcacg
     1561 aggtttgtat tgacgtgctt t
//
LOCUS       DAOQDO010001767         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_88289_length_1578_cov_3.572554, whole genome shotgun sequence.
ACCESSION   DAOQDO010001767 DAOQDO010000000
VERSION     DAOQDO010001767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88289_length_1578_cov_3.572554"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1578
                     /locus_tag="PKZ53_22505"
     CDS             94..>1578
                     /locus_tag="PKZ53_22505"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNJ43274.1"
                     /translation="MIESTSSPVNRRIAAIDIGSNSIHLIIAEIRPGQHLHIIDREKE
                     MVRLAAGTLQTHHLATRRMNVAIRVLQRFAELANAHQAHPILVTATSAVREAWNREDF
                     LNRVKKETGLDIEIIPGVEEARLISLAVSEVTDFHQKRGLIIDIGGGSTEFILTRGKE
                     PELLLSVKLGAVRLQEQFFAATKSGPPTRETIQATITHIRSSLARTVREIKQAGGFAA
                     VIGTSGTILALANAAAQTKTDRIEDRSPDYTPFSLNLSLAGLETINKQLHKTSLKERR
                     RMPGLDSRRADIIIAGGILLETILKELGAKELTTCDWSLREGIILNYLRENEPHILSA
                     QAHREPASAQPSLFQLQNQESIRARSVLSVARRYEFEERHSHHTAYLARRLFDETQLL
                     HKLGYVERELLEYAAILHDIGYHIAHNSHHKHAMYLIRHAELPGFHSHEVAILANIAR
                     YHRGSTPKSKHVEFMALTPFQREVVRKLSAILRIADGLDRRHLGV"
BASE COUNT          406 a          459 c          378 g          335 t
ORIGIN      
        1 cagccctgag cccgaagtct tcagccccaa gcccgttttc ttcagccctg agcccaatgt
       61 cttcacctga cccccgaact cgaagaggca tttgtgattg aatcaacatc atctcctgtc
      121 aatcgccgga tcgcagccat tgatatcggc tcgaactcaa tccacctgat cattgctgaa
      181 attcgtcctg gccagcacct gcatattatt gaccgggaaa aggaaatggt ccgactggca
      241 gccggaacgc tccaaactca ccatctggca actcgccgca tgaacgtcgc gatccgggtt
      301 ctgcagcgat ttgcagaact ggccaatgcc catcaagcac atccgattct ggtcacagcc
      361 accagtgccg tccgcgaagc ctggaaccgc gaagactttc tcaaccgggt caaaaaagaa
      421 accggcctcg atattgaaat cattcctggg gtcgaagaag cccgcctgat ttcgctggcg
      481 gtcagtgagg taaccgattt tcatcaaaaa cggggattga tcattgatat cggcgggggg
      541 agcaccgagt ttatcctgac gcgtggcaaa gaaccggaac tactgttgtc agtcaaatta
      601 ggggccgttc ggcttcaaga acaatttttt gccgccacca aatcaggacc tccgacccgc
      661 gaaacaatcc aggccacaat tacccatatt cggagcagcc tggcgcgaac ggttcgtgaa
      721 atcaaacagg ccggggggtt tgccgctgtg attgggacat ctggcacgat tctggcgctg
      781 gccaatgcag ccgcccaaac caaaactgac cggattgaag atcggtcacc agattacact
      841 ccatttagtc tcaatctgtc gctggccgga ttggaaacaa tcaacaaaca actccacaaa
      901 acatcgctca aagaacgccg tcgaatgccc ggtcttgata gtcgccgcgc tgacatcatc
      961 attgcgggtg gaattttgtt ggaaacgatc ttgaaggaac tcggcgccaa agaactcaca
     1021 acctgtgact ggtcactcag ggaagggatc atcctcaatt acctgcgtga aaatgaacca
     1081 cacattctct cagcccaggc tcaccgtgaa ccagcctcgg ctcagccctc gctattccag
     1141 cttcaaaacc aggagagtat tcgagccagg agtgtgctgt cagtggccag acggtatgaa
     1201 tttgaagaac gtcactctca ccacactgcc tatctggccc ggcggctctt tgacgagacc
     1261 caattgctgc acaaactggg ctatgtcgaa cgggaattac tcgaatatgc ggcaattttg
     1321 cacgacattg gctaccacat tgcccacaac agccaccaca aacacgcgat gtacctgatt
     1381 cggcatgccg aactcccagg atttcatagc cacgaagtcg ctatactggc caacattgca
     1441 cgttatcacc gtggatcaac cccgaagtcc aaacacgtcg aatttatggc tctgacgcca
     1501 tttcagcgcg aagtggttcg taaactatcg gccatcctga gaatcgcgga tgggcttgat
     1561 cgccgacatt tgggcgtg
//
LOCUS       DAOQDO010001768         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_88291_length_1578_cov_3.482600, whole genome shotgun sequence.
ACCESSION   DAOQDO010001768 DAOQDO010000000
VERSION     DAOQDO010001768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88291_length_1578_cov_3.482600"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1342)
                     /locus_tag="PKZ53_22510"
     CDS             complement(134..1342)
                     /locus_tag="PKZ53_22510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNJ43275.1"
                     /translation="MSASAVHYKIVIIGGGSAGLTVASRLSKEIPGGTDHPQIAIIEP
                     STKHYYQPMWTLVGAGVFPKEVTEREEADFIPPGTKWIHDAVETFFPEENYVVTRNGK
                     QVGYDYLIVAPGIQLDWEKVKGLKDAVGKNGVCSNYSYGTVNTTWENIKAFSGGTAIF
                     TQPPPPFKCGGAPQKIMYLADDYFRQSGVRDKTKIIYASAAATSFQVKKYADSMDQVI
                     ARKGIETKYRHNLIEIRPEKKEAVFQHLDTQEIVTLHYDMIHATPPQSAPDFIKRSPL
                     ANEAGWVDVNKETLQHVRYPNIFSLGDASSLPTSKTGAAIRKQSPVLVRNLEAMMRGV
                     PFPARYTGYTSCPLVTGYGKLVMAEFDYDLNPQETFPFDQSKERLSMYLVKKYVLPEL
                     YWHGMLKGRA"
BASE COUNT          355 a          367 c          420 g          436 t
ORIGIN      
        1 gctattgaac aaccgaccct gcgggcctca aacccttatc ctggcgctta tggggctatc
       61 ccctcgggtg gtactgactt tttgattgct cttttctgtg tattgcgtgg tttgttactt
      121 tgaaattcaa tatttatgcg cgtcctttga gcattccgtg ccagtagagt tcaggtaaca
      181 catacttttt gaccagatac atgctgagtc gttccttgga ttgatcaaat gggaatgtct
      241 cctggggatt gagatcataa tcaaactcag ccatcaccag cttgccgtac ccggtcacga
      301 gcgggcagga ggtatagcca gtgtagcggg cagggaaggg aacgccccgc atcatggctt
      361 ccagattgcg caccagaacc ggagattgtt tgcgaatcgc cgccccagtt tttgaagtcg
      421 gcaggctgct ggcatcgccc aaactgaaaa tgttgggata gcgcacgtgc tgcaaggtct
      481 ctttattgac atctacccag ccggcttcat tggccagcgg gctgcgcttg atgaaatccg
      541 gagctgattg cggtggggtc gcgtggatca tgtcatagtg cagggtgaca atttcctggg
      601 tatcgagatg ctgaaagacg gcttcttttt tctcgggtcg aatctcgatc aggttgtgac
      661 ggtatttggt ctcaatccct ttgcgtgcaa tgacttgatc cattgaatca gcgtattttt
      721 tcacctggaa actggtggct gcggcagacg cataaatgat ctttgttttg tcgcggacac
      781 cagactgtcg aaaataatca tcggccaggt acataatttt ttgcggggcg cccccgcatt
      841 taaatggcgg tggaggctgt gtaaagatgg ccgtgccgcc tgaaaacgct ttgatatttt
      901 cccaggtcgt gttgactgtt ccgtacgagt agttgctgca caccccattt ttgccgacag
      961 catctttcaa gcctttcacc ttctcccaat caagctggat gcctggcgcc acgatcaaat
     1021 agtcatatcc gacctgtttc ccatttctgg tcacgacata attctcttca ggaaaaaaag
     1081 tctcaacggc gtcgtgaatc cacttggtcc ctggcggaat aaaatcggct tcttcgcgtt
     1141 cggtcacttc tttgggaaaa accccagccc caaccagtgt ccacatcggc tggtaataat
     1201 gtttggtcga aggttcgatg atggctattt gtgggtgatc ggtgccacca gggatttctt
     1261 ttgatagccg ggaggccacg gtcaggccgg cgctaccgcc accgataatg actattttgt
     1321 agtgaactgc tgaagctgac ataatggatt tcacctgtgg gtattgagtt gtgtgtgggc
     1381 tttgaaggag tggatctgga aatgcaggaa agccgaagaa tatttgtaaa atatggaaaa
     1441 cacgctcaaa acacaaggta aaccaaatcg ggttccgggt tccgggtttt cggaagggat
     1501 gagggatgaa ggatgaggga tgaacaaatc acccggtcat cttgtcaggg ttccgggatt
     1561 tcaaactttt gtcctttt
//
LOCUS       DAOQDO010001769         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_88848_length_1573_cov_3.379447, whole genome shotgun sequence.
ACCESSION   DAOQDO010001769 DAOQDO010000000
VERSION     DAOQDO010001769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88848_length_1573_cov_3.379447"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1197)
                     /locus_tag="PKZ53_22515"
     CDS             complement(118..1197)
                     /locus_tag="PKZ53_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HNJ43276.1"
                     /translation="MMNTQMKPQIQMLPVPDHYDFKHAQRAEFNVNDANALIRKAEIW
                     KQKHGLKPVGSDKTKVHMLVIDDQVDFTFPSGSLFVAGRSGTGAMDAQNNLVQFIYRY
                     IHVLSQITCTMDTHLPYQVFFTSAHLRDDGSHPDAHTIVSADEYRRGKFQPNPAMAKQ
                     LGADLMWLRRQFIHYCEQLEASGKYQLYLWPYHCLLGSNGHRLAGVVEEARLFHSFAR
                     GAENRPEIKGGNPLTEHYSIFAPEVTTCWDSRPIPGAQKNTHLVKTLMEADVVLMAGL
                     ASSHCVRGSIADFLGEIQRQDPKLAKKVYILRDCTAAVVVPGGPDFTDEAERAFQNFK
                     DAGMHIVNSTDPIESWPEIQATGLC"
     gene            complement(1357..>1573)
                     /locus_tag="PKZ53_22520"
     CDS             complement(1357..>1573)
                     /locus_tag="PKZ53_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43277.1"
                     /translation="TLRGKCAVGNFLLVATDDGIIRMEPKFNQILKTREFPDTESFVD
                     AGCQLIAGRQGLYVVTSQEIRLLELRA"
BASE COUNT          379 a          418 c          387 g          389 t
ORIGIN      
        1 gtaagtcctt tctgaaaaaa ctgtccttcg tgcctggtat ttagtgcttt gaaactattg
       61 gataccaggc accaggcacc aggcactaaa ccctgaaccc cgaaccctga accctgatta
      121 gcacaatccc gtggcctgga tttccggcca gctttcaatc gggtcggttg aattgacgat
      181 gtgcattccg gcatccttga agttttggaa agcccgttcg gcttcgtcag taaagtcagg
      241 accacccgga accaccactg ccgccgtgca atcacgcaaa atataaactt tcttggccag
      301 ttttggatcc tggcgctgaa tttcacccag gaaatcggcg atgctcccgc gcacacagtg
      361 gcttgaagcc agacctgcca tcaataccac atcagcctcc atcagcgttt tgaccagatg
      421 ggtgttcttt tgagcgcctg gaattgggcg actatcccag caggttgtga cttccggagc
      481 aaagatcgaa tagtgctccg ttaaaggatt accacccttg atttcggggc gattttccgc
      541 acctcgcgca aagctatgaa acaaccgggc ttcctcgacc acacctgcca gccgatgtcc
      601 atttgaccca agcaggcagt gatacggcca caaataaagc tgatatttgc cgctggcttc
      661 gagttgttcg cagtagtgaa taaactgccg ccggagccac atcaaatctg ctcccagttg
      721 tttggccatc gccgggtttg gctgaaactt gccgcgccgg tattcatcgg ctgaaacgat
      781 ggtgtgggca tctggatggc tcccgtcatc acgcaggtga gcactggtga aaaacacctg
      841 atagggcagg tgggtgtcca tcgtgcaggt aatctgtgac aacacgtgaa tgtaccggta
      901 aatgaactgc accaggttgt tttgagcgtc catggcacct gttccagagc gaccagccac
      961 aaacagcgac cctgacggga aggtaaagtc cacctgatca tcaatcacca gcatatggac
     1021 tttggttttg tcgctgccaa caggcttcaa tccgtgtttt tgcttccaga tctcagcttt
     1081 gcgaatcagc gcattcgcat cattgacgtt aaattcagcc cgttgggcgt gtttgaaatc
     1141 atagtgatct ggaacgggta acatctggat ttggggtttc atttgagtat tcatcataaa
     1201 aagggctcct taaatcgagg gctgaagaaa acgggcttgg ggttaagggc ttggggctga
     1261 agattcgcag gctcagggct gaagacttcg ggttcaatgc aggattccca atttcttcag
     1321 ccctcagccc caagccctta gccctggttt ttggccttaa gccctaagtt ccaacaagcg
     1381 aatttcctgt gatgtcacca catacagacc ctggcgacca gcaatcagtt gacatccggc
     1441 atccacaaaa ctttcggtgt caggaaattc acgggttttg agtatctggt taaatttcgg
     1501 ctccattcga ataatgccat catcagtggc cacaagcaga aaatttccaa ccgcgcattt
     1561 tccgcgaagc gtc
//
LOCUS       DAOQDO010001770         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_88910_length_1573_cov_2.083004, whole genome shotgun sequence.
ACCESSION   DAOQDO010001770 DAOQDO010000000
VERSION     DAOQDO010001770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88910_length_1573_cov_2.083004"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKZ53_22525"
     CDS             <1..208
                     /locus_tag="PKZ53_22525"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ43278.1"
                     /translation="LEVSVSKHLTSVKHTFTHFQVTLHVFSCAINQGEIVTSNPRWVT
                     VDETSSLALTRTARKILEFLEKQS"
     gene            383..1552
                     /locus_tag="PKZ53_22530"
     CDS             383..1552
                     /locus_tag="PKZ53_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNJ43279.1"
                     /translation="MTNEAQPPKSSQRLDSIDLLRGVVMVIMALDHVRDFFSGNHIDP
                     TNLAQTSTALFLTRWITHFCAPVFVFLAGTAMFLSASKGKSKKELASFLVTRGLWLVF
                     LELTVVRFGWFFNFNYQLMVGQVIWAIGWSMVALGGLIFLPVWAVTTFGILMVAVHNL
                     FDNVQAEAFGRFDWVWMILHNTGTMEVFPGKSFAVSYPLIPWIGVMAAGYGFGQLYQL
                     EPAQRRRALYGLGSALVGLFIVLRGINIYGNPLPWSVQPSMVFTVLSFLNCEKYPPSL
                     LYLLMTLGPAILFLAYFDGSVSKIGQPFVTFGRVPLFYYVLHLPLINLIFHIDALRVL
                     GSLEFTGPFSIPEDYGYSLPVVYLVWMCIVLALFPACRWFADVKRRRKDPWLSYL"
BASE COUNT          316 a          394 c          394 g          469 t
ORIGIN      
        1 gcttgaggtt tcggttagca aacacctgac gagcgtcaag catacgttta cccattttca
       61 ggtcacactc catgtctttt cctgtgcgat caatcagggt gaaatcgtaa cttctaaccc
      121 tcggtgggtg acggtggatg aaacctccag cctggcctta acccgaacag cccgaaaaat
      181 cctggaattt ctcgaaaaac agagttgaca gtttcgatca aagccgacag gatgtgcctt
      241 cctgccgctc atccctactc gccgttgctg ttcccgtctc tttttttcag aagcgacctg
      301 ttggctggct tcaaagaaat cagaagccat tttgtttttc aagcccttct tcaactccca
      361 gcaacttttt ggaaatccag gcatgaccaa cgaagctcaa ccaccaaagt cttctcagag
      421 gcttgactcg attgacctct tgcgcggtgt tgtgatggtg attatggcgc ttgatcacgt
      481 ccgcgatttt ttctccggaa atcacattga ccccacaaac ttagcccaga ccagcacggc
      541 cttgtttctc acccggtgga ttacccattt ctgtgcaccg gtgtttgttt tcctggctgg
      601 aacggccatg tttttgtcgg cgtcgaaagg aaaatcaaaa aaagaactgg cttcctttct
      661 ggtaacacgg gggctctggc tggtgtttct ggaactgacc gtggtgcgat tcggctggtt
      721 ttttaatttc aattatcagt tgatggtcgg gcaggtcatc tgggcgattg ggtggtcaat
      781 ggttgccctg gggggactta tttttctgcc agtatgggcc gtcacaacct ttggaattct
      841 gatggtggcg gtccacaatc ttttcgacaa tgttcaggcg gaagcatttg gccgatttga
      901 ttgggtgtgg atgattcttc acaacaccgg gaccatggag gtttttccag gcaaatcttt
      961 tgccgtcagt tacccgctca tcccctggat tggcgttatg gccgccgggt atggctttgg
     1021 gcaattgtat caacttgaac cagcccaacg gcggcgggct ctctatgggt tggggagtgc
     1081 cctggtgggt ttgttcatcg tactgcgcgg aatcaatatc tatgggaacc cacttccctg
     1141 gagtgttcaa ccatcaatgg tctttaccgt gctttcattt ctcaactgtg agaaatatcc
     1201 gccgtcgctt ctgtacctgc tcatgaccct gggtccagcg attctgtttc tggcttattt
     1261 tgacggttcg gtcagtaaaa tcggtcaacc ctttgtgacc tttgggcgag taccgctgtt
     1321 ttactatgtg ctgcacctgc ccttgatcaa tttaatcttt cacattgatg ccctccgggt
     1381 gctgggttca ctcgaattca ccggaccctt tagcattccg gaggactatg gatacagttt
     1441 gccagttgtg tacctggtct ggatgtgtat tgtcctggcg ttgtttcccg cctgtcggtg
     1501 gtttgccgat gtcaagcgac gcagaaaaga cccatggctg agttatcttt aaaaatcatc
     1561 gcagtttgta ggc
//
LOCUS       DAOQDO010001771         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_89287_length_1569_cov_3.732497, whole genome shotgun sequence.
ACCESSION   DAOQDO010001771 DAOQDO010000000
VERSION     DAOQDO010001771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89287_length_1569_cov_3.732497"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1420
                     /locus_tag="PKZ53_22535"
     CDS             128..1420
                     /locus_tag="PKZ53_22535"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNJ43280.1"
                     /translation="MNKEFFKTYGLILFLTVVAFGIAFQFAEPAPPRKVVMATGLESG
                     AYYKFALRYRDYFAQNGIQLEVLQTSGSVENLELLKTHGKGVDIIFAQTGTGDPAATP
                     DFTSVASVFYEPLWVFYRGEQPIQRLVELKGKRVAVGAEGSGTRMVSLKILSDNGLTV
                     ATTQILSLGGNQAAEALKTSQIDAAFFVGAPEAPVINDLLRSEGIRLMNFERVEAYKS
                     RYRYFSSVILYEGVVDMQKNIPAEDVVLLASTASIVVRENFHPALVNLLLQAANEIHR
                     KETGMLEKTGQFPSSDFIQFPLNDEAVRYFRFGPSFLTRHLPFWAATAVDRLKFVLLP
                     LLTMLIPLLRIAPPIYAWRTRRKIYTWYGMLIALENKVVNPEQNQDFAQILVELDDLE
                     REVAKIVVPLSYTDEVYNLRLHIRLVREKAMEPRTKSE"
BASE COUNT          401 a          409 c          368 g          391 t
ORIGIN      
        1 aaaacccgcc ccgccgttcg agcacaggtc accaaacaac gagtcaaagc cgtcccgccc
       61 cagtcagccc aacgaccttc cttgaaaaaa cccaaacccc aggatcaaac gacccacgaa
      121 atcacccatg aataaagaat tcttcaagac ctatggcttg atcttgtttt tgaccgttgt
      181 cgcttttggg attgcctttc agtttgctga accagcgcca ccacgcaaag tcgtgatggc
      241 aacagggctc gaaagcggcg cttattacaa gtttgccctc cgctatcgag attattttgc
      301 ccaaaatggg attcagcttg aggtgctgca gacttcgggt tcagtcgaaa acctggaact
      361 gctcaaaacc cacggcaaag gcgtggatat cattttcgcc caaactggaa ccggggatcc
      421 ggctgctacc cctgacttta cttcagtagc cagtgttttt tatgaaccgc tctgggtgtt
      481 ttatcgcggg gaacagccga ttcagcgact ggtggaattg aaagggaaac gagttgcggt
      541 aggggcggaa ggaagcggca cccggatggt ctcgctgaaa atcttatctg acaatggttt
      601 aactgtcgcc acgacccaaa ttctctcact tggaggaaat caggctgctg aagcattaaa
      661 aaccagtcaa attgacgcgg ccttttttgt cggtgcgcca gaagccccgg tcatcaatga
      721 tttgctgcgg tctgaaggca tccggttaat gaattttgaa cgcgttgaag cctacaaaag
      781 ccgctatcgc tatttttcca gcgtcattct gtatgaaggc gtggtggata tgcagaaaaa
      841 cattccagcc gaagacgtgg tcttgctggc gtcaaccgcc tcaattgtcg ttcgagagaa
      901 tttccacccg gcgctcgtca atttgttgtt acaggccgcc aatgaaattc atcgcaaaga
      961 aacgggaatg cttgaaaaaa ccggtcagtt cccgtcgtct gactttattc aatttccgct
     1021 caatgacgaa gcggtgcggt atttccgatt cggaccatca tttctaaccc gccatttgcc
     1081 gttttgggca gccacggcgg ttgaccggtt gaaatttgtg ctcctgccgc tgttgacgat
     1141 gttgattcca ctccttcgga ttgccccacc gatttatgcc tggcgaaccc gccgaaaaat
     1201 ctatacctgg tacggcatgc tcattgccct tgaaaacaaa gtggtgaatc ctgagcaaaa
     1261 ccaggatttc gcccagattt tggttgaact tgatgacctg gagcgcgaag tcgccaaaat
     1321 tgtggtgccg ctttcctaca ctgacgaagt gtacaatttg cgcctccaca tccgactggt
     1381 ccgggaaaaa gcgatggaac cgcgaacaaa aagcgagtag tcaatccatc tgatcaagga
     1441 tctttcgaga attccagcat ttttcaccgc agaggacaca gagggacaca gaggaaagct
     1501 gaaaacaaaa aaccggttca ttcttcattc ttcattcttc attcttcatt cttcattctt
     1561 cattcttca
//
LOCUS       DAOQDO010001772         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_89513_length_1567_cov_2.824074, whole genome shotgun sequence.
ACCESSION   DAOQDO010001772 DAOQDO010000000
VERSION     DAOQDO010001772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89513_length_1567_cov_2.824074"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1274)
                     /gene="hemW"
                     /locus_tag="PKZ53_22540"
     CDS             complement(75..1274)
                     /gene="hemW"
                     /locus_tag="PKZ53_22540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNJ43281.1"
                     /translation="MTQLNQEPQSQSFKKPEPGIYLHLPFCHSKCTYCGFVTRSFDDD
                     LSTQYLAALEQEIRAFPPSGTVVSRERPVIDTIYLGGGTPSILTPKAIGRLLDACRER
                     FELSEQAEITIEVNPGDTQADRLEGFRTCGINRASLGVQSFLDHQLRATGRDHTADQS
                     RAAVAALRAAGFDNLSLDLIAGLPNQTMDEWKYNLHQALALKPEHLSLYLLEVKEGTK
                     LADQILNGKLPTPDDDLAAEMYETLLDMLEQAGFEAYEISNFARSRALRSRHNLKYWN
                     DIPFFGFGVGAHSYDGDERYRNPSSIHDYLQLMSTQGMAVIDRTSRNPEEKFYEALMM
                     GLRLREGVHLPSLTSQYTISLSPTFTQELQRLLEAEVIELQDDQLRLTRRGVLVSNEV
                     FVALMPE"
     gene            1273..>1567
                     /locus_tag="PKZ53_22545"
     CDS             1273..>1567
                     /locus_tag="PKZ53_22545"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNJ43282.1"
                     /translation="MSKFHLRWIFKSVVMVGLLYFLLRKVQLQPLIEALRRANPSFLA
                     IAVVVGVAMVVVRVYKWQALLHRLPATVAPHPVSFRQAWASVLGGLAFGLITPA"
BASE COUNT          357 a          392 c          421 g          397 t
ORIGIN      
        1 tgacacctga ccaggtgact atgtgactgg gtgacacgag agaaatggtc tgggctggcg
       61 actcagcact cacttcattc aggcatcaag gcgacaaaaa cttcatttga gaccagaaca
      121 ccgcgtcgag tcagccgaag ctgatcatct tgaagttcaa tcacttcagc ttccaaaaga
      181 cgttgcaatt cctgagtaaa ggttgggctg agggaaatag tatactggct ggtcagcgaa
      241 ggcaggtgaa ccccctcgcg taagcgcaac cccatcatca aggcttcata aaatttctct
      301 tccgggttcc gtgaggtacg gtcaatgacg gccatgccct gggttgacat caactgcaga
      361 tagtcatgga tcgaagacgg gttgcgatat cgctcgtcgc catcgtagga atgggcgccg
      421 acaccaaatc cgaaaaacgg tatatcgttc caatatttca ggttatggcg tgaccgaagt
      481 gctcgggacc gagcaaaatt tgagatttca taggcttcaa agcctgcttg ttcgagcata
      541 tccagtagcg tttcatacat ctcggcggcc agatcatcat ctggtgttgg cagtttcccg
      601 tttaggatct ggtcagccag cttggttcct tctttcacct ctaataaata gagcgagagg
      661 tgttcaggtt tgagtgccag tgcctggtgt aaattatatt tccattcatc catcgtttga
      721 tttgggagac cagcaattaa atcgaggctc agattgtcaa acccggcagc ccgtaacgca
      781 gccacggcgg cgcgtgactg gtctgccgta tgatcacgcc cggttgcccg aagctgatgg
      841 tcgagaaatg actgcacgcc aaggctcgcc cggttgatgc cgcacgtacg aaatccttcc
      901 agccggtcgg cctgggtatc gccgggatta acttcaatgg taatttcagc ctgctcactc
      961 agttcaaacc gttcccgaca ggcatcaagc aatcggccaa tggctttcgg cgtcaggatc
     1021 gagggtgttc ctcctcccaa ataaatggta tcaatgaccg gtcgttcgcg gctaaccacc
     1081 gttcccgagg gcggaaaggc ccggatttct tgttccaggg ctgccagata ttgtgtggat
     1141 aaatcatcat caaaactacg ggtgacaaac ccgcaatagg tacactttga gtgacaaaat
     1201 ggcagatgga ggtaaattcc aggttctggt tttttgaaac tttgagattg aggttcttga
     1261 ttgagttgag tcatgtccaa atttcatctg cggtggatat ttaagtcggt cgtgatggtt
     1321 gggttgctct attttctgct tcgcaaagtg caacttcaac cgctgatcga agccttacgc
     1381 cgggcgaatc cttcctttct ggccattgcg gtggtggtcg gagtcgccat ggtcgtggtc
     1441 cgggtttata agtggcaggc gctcttgcac cgactgccag caacggttgc cccccacccg
     1501 gtttcatttc gacaggcctg ggcatcagtg ttgggcgggc tggcctttgg gttgattacg
     1561 cccgcca
//
LOCUS       DAOQDO010001773         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_89609_length_1566_cov_3.178028, whole genome shotgun sequence.
ACCESSION   DAOQDO010001773 DAOQDO010000000
VERSION     DAOQDO010001773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89609_length_1566_cov_3.178028"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKZ53_22550"
     CDS             <1..308
                     /locus_tag="PKZ53_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43283.1"
                     /translation="YSFQYGNLGANDHSCGIGAARNEDNINWEDTNSTSKITYTQQYF
                     DEEKTYTVVFEQSADQYTLTFGDEHESYFCGMRATLPKKVIVKKIGTTYTGSAVLGY"
     gene            700..1455
                     /locus_tag="PKZ53_22555"
     CDS             700..1455
                     /locus_tag="PKZ53_22555"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43284.1"
                     /translation="MLTRRLFLTRSLCLPLLPTLPSWNAFDWPQSVEGPVIQKFTPAP
                     GDESPDVKTVLDTVRTELKTGKTTAERLLTDPKYLDLHPWPRFRELICEFGQESHLTM
                     VCPDEPGIRLSVTCAVIDAQGKRVPEAQVYLYHTSSQGLYARQAAHISAMEGDRRHAR
                     LFGYLRTDQNGMFSVQTIRPAGYPGTELPAHIHVEIKSGDLSPLITEIRFEDDPRLTP
                     ALRAQSIREGFRICSVVSKNQSEHRVETKFQLR"
BASE COUNT          417 a          398 c          363 g          388 t
ORIGIN      
        1 tctatagctt tcagtatgga aaccttggtg ccaatgatca ttcctgcggg attggtgccg
       61 cccgcaatga agacaatatc aactgggaag ataccaattc aacctcgaaa attacctaca
      121 cccagcagta ttttgatgaa gagaaaacct acaccgtcgt gtttgaacaa agcgccgacc
      181 agtacaccct gacctttggc gatgagcatg aatcctattt ttgcggcatg cgggcaacgc
      241 tcccgaaaaa ggtgattgtg aaaaagattg gaacgactta taccggctca gccgtgcttg
      301 gatattgatc tcgttcaggc aggcacagca ataccatttt ggaatgaatt tatcgtatta
      361 gcaaaaggct aagttattag gagaattggg gtttcctgct ccctaacccc gacgccctga
      421 ccccgaaatg gtataagtgg gatgaacctc cagactgaga cgtaactgca ttaccagaca
      481 ccagaaaaga attgaagaaa tttgcccatc tgcctgtcta actatttagg gctaagggta
      541 tcgggctaag ggctgaggga aatacaattt tatcagcagc ttagccttat gctaccgcga
      601 tagaatcact tcaaaatggt tgaaaaacca ggtttggcca ctcagtttga cgggtggctc
      661 attttcccaa aaacgaaaag ttcttttcag gtgtctccta tgctgactcg acgtttgttt
      721 ctcaccagat cgctatgcct gcccctgctt ccaactctcc cctcgtggaa tgcctttgac
      781 tggccgcaat ctgtcgaagg gcctgtcatc caaaaattca cacccgctcc gggtgatgaa
      841 tccccagacg tcaaaaccgt tctcgataca gtcagaactg agttaaaaac cggtaaaacg
      901 accgccgagc gactgttaac tgaccccaaa tacctggact tacatccctg gccccggttt
      961 cgggaattga tctgtgagtt tggtcaggaa tcacatttga cgatggtttg ccctgatgaa
     1021 ccaggaatcc gactctctgt aacctgtgcc gtgattgatg cacaaggaaa acgcgttcca
     1081 gaggctcaag tgtacctcta tcacaccagt tcccagggac tttatgcccg gcaggcggct
     1141 cacatttcgg caatggaagg tgaccgtcgc cacgcccggc tgtttgggta tttgcgtact
     1201 gaccagaatg gaatgttttc agtgcaaacc attcgaccgg ctggatatcc aggaactgaa
     1261 ctccccgctc atattcatgt agaaatcaaa agcggagatt tgtcgccgct catcactgaa
     1321 atccggtttg aagatgaccc acgattgaca cctgccctgc gagcgcaatc catccgcgaa
     1381 gggtttcgaa tttgttcggt tgtatcaaaa aatcaatctg agcaccgggt tgaaaccaaa
     1441 ttccagcttc gatgaccgga accctcattg ggcagagggt tttagaaata agccttcccg
     1501 acaaccgttg aattgaccgc actggaccgg gtttgtatag ttgcagccgg ctcaagaatt
     1561 tttccc
//
LOCUS       DAOQDO010001774         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_89848_length_1564_cov_2.768721, whole genome shotgun sequence.
ACCESSION   DAOQDO010001774 DAOQDO010000000
VERSION     DAOQDO010001774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89848_length_1564_cov_2.768721"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1516)
                     /locus_tag="PKZ53_22560"
     CDS             complement(56..1516)
                     /locus_tag="PKZ53_22560"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNJ43285.1"
                     /translation="MLIYNQSLALLTDLYQLTMAYGYWKSGIGQKEATFHLFFRKNPF
                     NGGYCVAAGLAHVITLLQHFKFDASDVDYLASLTGRDGNPLFEDKFLQYLQEMSFSCD
                     LDAVPEGTIVFPSEPLIRIQGDIIQTQILETALLNIINFQSLIATKAARVCMAAEGEP
                     VLEFGVRRAQGIDGGLAASRAAYIGGCAATSNVLAGKVYGIPVKGTHAHSWVMCFDDE
                     LEAFQTYAKAMPNNCVFLVDTYDTLEGVKKAIEVGKWLRSEGHEMIGIRLDSGDLAYL
                     SIEARKLLDEAGFPHATIVASNDLDETIINSLKQQGAQIGMWGVGTKLITAYDQPALG
                     GVYKLSCIRDRDGEWHHRVKLSEQAIKISNPGIQQVRRYFHNGESVADVIYDVSTDLS
                     SGCVMVDPMDMTRQKRLTAGTPFEDLLVPVFRNGSCVYQNPPIAEIRAQTHQNLEKFH
                     SGVKRFVNPHQYPVGLEKSLHTLKTDLVLKARGVKS"
BASE COUNT          378 a          401 c          384 g          401 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattctca attctctagc
       61 ttttgactcc gcgagccttc aaaaccagat cggtcttgag cgtgtggagt gatttttcaa
      121 gtccgaccgg gtactggtgt ggattgacaa accgcttgac gcccgaatgg aatttctcca
      181 ggttctggtg tgtttgggcg cggatttcag caatcggcgg gttttgatac acacagcttc
      241 cattgcggaa aacgggaacc agcagatctt caaatggggt tccggcggtc agtcgtttct
      301 ggcgggtcat atccattgga tcaaccatca cacatccact cgataaatca gtactcacgt
      361 cataaataac gtcggccaca ctttcaccgt tgtgaaagta gcgtcgaacc tgttggattc
      421 cagggtttga aattttaatc gcctgttcgg aaagtttgac ccggtgatgc cattcgccat
      481 cgcggtcccg aatgcaggac agcttataga ctccacccag ggccggctga tcatacgcgg
      541 tgatgagttt ggttccgacg ccccacatcc caatttgagc gccctgctgt ttgaggctgt
      601 tgatgatagt ttcatccagg tcattactgg ccacaatcgt ggcgtggggg aacccggctt
      661 cgtccaggag ctttcgggct tcgatgctca gataggctaa gtcacctgaa tccaatcgaa
      721 tgccaatcat ttcatgacct tcactgcgca gccattttcc aacctcgatg gcttttttga
      781 caccttcgag cgtgtcatag gtatcaacca gaaacacaca attattgggc atggctttgg
      841 cgtaggtttg aaaggcttcc agttcatcat caaagcacat cacccagcta tgagcgtgcg
      901 tgcctttgac cggaattcca tacactttcc cagccagcac atttgaagtc gcggcacatc
      961 cgccaatata agcggcccgg ctggcggcca gtccgccgtc aattccctgg gctcgccgaa
     1021 caccaaattc gaggactggc tcaccttcgg cggccatgca cacccgggca gccttggtgg
     1081 caatcagtga ttgaaaattg atgatattta acaaggctgt ttccagaatc tgggtctgga
     1141 tgatatcgcc ttgaatccgg atgagtggct cagaaggaaa cacgatagtg ccctcaggca
     1201 cggcgtccag gtcacatgaa aaagacattt cctgcagata ttgcagaaac ttgtcttcaa
     1261 acaatggatt cccgtctcgt ccagtcaacg acgccaggta gtcgacgtct gaagcgtcaa
     1321 atttgaagtg ttggaggagg gtaatcacat gtgccaggcc agcagctaca cagtaaccgc
     1381 cattaaatgg atttttgcgg aaaaacagat gaaaggtggc ttctttctga ccgatccccg
     1441 atttccaata gccataggcc atcgtgagtt gatacaggtc ggtaagcaaa gccagggact
     1501 ggttatagat caacatgggg tgaacctcag tgtggaaaac aaagacttca ggctgaagac
     1561 ttgc
//
LOCUS       DAOQDO010001775         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_89850_length_1564_cov_2.630219, whole genome shotgun sequence.
ACCESSION   DAOQDO010001775 DAOQDO010000000
VERSION     DAOQDO010001775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89850_length_1564_cov_2.630219"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKZ53_22565"
     CDS             complement(<1..295)
                     /locus_tag="PKZ53_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43286.1"
                     /translation="MKSRLFQISILCLGIALWVFPPVSAFQQKKDVPPPPLPLPTGTG
                     QKSPEPAGQQEDEDTVIRIGTELVNVPFSVTNKQNRYINDLTQEQIQVLEDGKE"
     gene            complement(419..1423)
                     /locus_tag="PKZ53_22570"
     CDS             complement(419..1423)
                     /locus_tag="PKZ53_22570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ43287.1"
                     /translation="MHKVIRFSAGKLLYLLVLLTIVSLVPVPSGFSASTTAQPQKETT
                     GGGQDEPLKGGGVTNVTLPVTITDNDDRFIYGLTKEDFEVFEDNKKQDIDKFEASEEL
                     PLYIAILLDTSGSVKAKLKFEKEATISFLQTVLRRRKDQALVVTFDSTVQLRQDFTDN
                     TELLAKAIDSIRASGTTSLYDAVYRICEEKMVGVPTPRKVIIILSDGDDTSSTHTLEE
                     AIDIAQQSEVVIFGISTKGAGFFGVSGGQVYNTDDKELRRLCLETGGDITFPSKVIDL
                     ERAFQRYEKTARRYYLLSYEPEDPTRPGYRKIEVRLTNRKGLRVKTRKGYTVPKTEAN
                     "
BASE COUNT          361 a          367 c          383 g          453 t
ORIGIN      
        1 gttccttgcc atcttccaaa acctggattt gctcctgggt caggtcattg atgtagcgat
       61 tttgtttatt ggtcactgaa aagggaacgt tcaccagttc cgttccaatt cgaatcaccg
      121 tatcttcgtc ttcttgttga cctgctggct ctgggctttt ttgcccggtt ccggtgggta
      181 acggcaacgg cggcggtgga acatcctttt tttgctgaaa ggcactcacg ggtgggaaga
      241 cccacagtgc gatccccagg cagagtatac tgatttggaa cagtctggat ttcattagct
      301 taacctcacg gaagaatgaa gaattgagaa tgaagagatt gcctggtgca agctattgat
      361 ttctaaacac tcgccactac taaccagttt cttcattctt cattcttcat tctcaattct
      421 aattggcttc tgtcttaggc acggtatagc cctttcgggt cttgacacgc aaccctttgc
      481 gattggtcaa cctgacttca attttgcggt agcctgggcg agtgggatct tcaggttcat
      541 agctgagcag gtaatagcgc cgggcggttt tttcatatcg ctggaaagcc cgttccaggt
      601 caatcacttt ggatggaaag gtaatatcgc caccggtttc caaacacaga cgccgtagtt
      661 ctttgtcatc tgtgttgtaa acctgcccgc cactaactcc gaaaaatcct gcccccttgg
      721 tactgattcc gaaaatgacc acttcgctct gttgagcgat gtcaatggct tcttcaagcg
      781 tgtgcgtgct tgatgtatcg tcgccgtcac tcaagatgat gatgaccttg cgtggggttg
      841 gcacaccaac cattttctct tcgcaaatcc ggtagaccgc gtcataaagc gaggttgtgc
      901 cgctggcccg gatgctatca atggctttgg ccaataattc agtgttatca gtaaaatcct
      961 gccgaagttg cacggtggaa tcaaaagtca ctaccagggc ctgatctttg cgtcgtcgaa
     1021 gcacggtttg caaaaatgag atcgtggctt ctttttcaaa cttgagcttg gctttcacac
     1081 taccgctggt gtccagcaaa atagcaatat aaagcggtaa ttcttctgaa gcttcaaatt
     1141 tatcaatgtc ttgcttttta ttgtcttcaa agacctcaaa atcttctttg gtcaaaccat
     1201 agataaagcg gtcatcattg tcagtgatcg tgacaggaag tgtcacattg gtcacaccgc
     1261 cgcccttgag cggttcatct tgtcctccgc cagttgtttc tttctggggc tgggcggtgg
     1321 ttgatgccga aaaacccgac gggacgggga caagcgaaac aatggtcagc aaaacaagaa
     1381 gatacagcaa cttaccggct gaaaagcgga ttactttgtg cataacaact ttcgagatgg
     1441 gaaattggat ttaaggatga caggttgatg tttcaaccca ggcgcatgat gtcttgtttc
     1501 tcccaggaag ccaagccgct ttggtgtttg gggactgggg actggtgctt ggttcttggt
     1561 gctt
//
LOCUS       DAOQDO010001776         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_89911_length_1563_cov_3.934350, whole genome shotgun sequence.
ACCESSION   DAOQDO010001776 DAOQDO010000000
VERSION     DAOQDO010001776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89911_length_1563_cov_3.934350"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PKZ53_22575"
     CDS             <1..1473
                     /locus_tag="PKZ53_22575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43288.1"
                     /translation="LIRQLLIEHTVFAVTGGVMGVWLAYLSLGLLVKISPDLVARFNT
                     VSISGPVLWYTVGATTLAAIVFGVLPALLGTKTDLIEVLKQGATTVSQGTGQKLFQSG
                     LVVVEISLAFVLMVGAGLLVQSFVKLNQVKPGYDLDPVLTMDVYLPGARYTQAEQRIA
                     FYRSAIERLRSIPGVTVAAAVQGPPLKGPIYSDPVLIEGQPAPRPGEVPFLRQNVVTA
                     DYFRTLGIQVVQGRSFSETETWENHNVIVINQSFARRFWPGENAIGKRLKTGEEKPWL
                     SVVGVIADVAQDRFDRPGIEEMYYPYVNPAETLPVFGMNLVVRSNLDPESLIPAIRQQ
                     LRQIDPVVPINRVLTLRQLADQVNGRTRLMVVLLNLFAGLALILALTGVYAVMTFFVT
                     QRQQEIGIRMAMGATQHQVLKLVIGRCMTLCAIGLALGLGVSQLVGQVLNSQVFSISV
                     FDVRTFVGMAVVLGFSTLIAVYFPARRATEIDPIAVLRGE"
BASE COUNT          328 a          416 c          424 g          395 t
ORIGIN      
        1 ctcatccgcc agttactgat cgaacacacc gtttttgccg tcacaggtgg cgtgatgggt
       61 gtctggctgg cctacctaag ccttggactc ctggtcaaaa tcagccctga cctggtggct
      121 cgattcaaca cggtatctat cagcggaccg gtgctctggt acaccgtcgg cgccacgacg
      181 ctggccgcga ttgtctttgg tgtgttgccg gcactccttg gcaccaaaac tgacctgatc
      241 gaagtcttga aacagggcgc caccactgtt tcacaaggca ctggccaaaa actttttcaa
      301 agtggactcg tcgtggtgga aatcagtctg gcgtttgtgt tgatggttgg ggcgggcctg
      361 ctggttcaaa gttttgtcaa actcaatcag gtcaaacctg ggtatgatct ggacccggtg
      421 ctcacaatgg atgtctattt gcctggcgcc cgatataccc aggccgagca acgcatcgcc
      481 ttctatcggt ctgcgattga gcggctccgg tcaattcctg gggtaacggt tgccgctgcg
      541 gttcaagggc caccgctcaa aggcccgatt tattccgacc cggtgctgat tgaaggtcaa
      601 ccggcaccac gccctggcga agtgccgttc cttcgccaaa acgtcgttac ggctgattac
      661 tttcgcacat taggtattca ggttgttcaa ggccgctctt tttcggaaac ggaaacctgg
      721 gaaaaccata acgtcattgt gatcaatcaa tcctttgccc gccggttctg gccgggcgaa
      781 aacgcgattg gaaaacggct caaaaccggt gaggaaaagc cctggttatc agtggtcggc
      841 gtcattgccg acgtggctca ggatcgcttt gatcgtcctg gaattgagga aatgtattac
      901 ccctatgtaa atccggctga aacacttcca gtctttggaa tgaatctggt ggtgagatca
      961 aatctagatc ctgagagttt aatcccggcc atccggcagc aactacggca gattgatcca
     1021 gtcgtgccaa tcaaccgggt cctgaccctg cggcaactgg cagatcaggt caatggtcgc
     1081 acccggctga tggtggtctt gttgaacctg tttgccgggc tcgcccttat cctcgccctc
     1141 accggggtgt atgccgtcat gacctttttt gtgacccagc gccagcagga aattggcatt
     1201 cgaatggcga tgggcgccac acagcaccag gttttgaaac tggtcattgg ccgctgtatg
     1261 acgctgtgtg ccattggact tgccctgggg cttggcgtct cgcaactggt tgggcaggtg
     1321 ctcaactcgc aggttttttc aatctcagtg tttgatgtgc gaacctttgt cggaatggct
     1381 gtggtactgg gcttttcaac cctgatcgcg gtgtattttc ccgctcgccg cgcaacagaa
     1441 attgatccga ttgccgtcct gcgcggggaa taacaccatt ttgcaatgaa gttcttgtat
     1501 tacgggaagg ctaagtacct taccgaaaat ttccagacat tttaaccacg gaaaacacgg
     1561 aaa
//
LOCUS       DAOQDO010001777         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90044_length_1562_cov_2.512276, whole genome shotgun sequence.
ACCESSION   DAOQDO010001777 DAOQDO010000000
VERSION     DAOQDO010001777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90044_length_1562_cov_2.512276"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKZ53_22580"
     CDS             <1..229
                     /locus_tag="PKZ53_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43289.1"
                     /translation="EANQRHRRVELKEHLRDFFSSAHRLNPNRWITQTIERLGFALLD
                     GELAAEVRPETIIAVALKIAAQFAAEGCAGW"
     gene            465..1076
                     /locus_tag="PKZ53_22585"
     CDS             465..1076
                     /locus_tag="PKZ53_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43290.1"
                     /translation="MKSLMWKTFYRWLKTKELNIVYLGPPLAGKHANLRHLQALALQE
                     FGVKSSTTNELILEGSQLLPLLGMDSALVGMQPIEFRIQTIPISTYRDPVRDDLLRSM
                     DAAVMVFDSQRGRMDSNAAINWNLKSWMINLNRDMSAIPQVIQYNKRDFPRLETIEVL
                     EQRLNPQQLPYVEASASKGVGVTETLRILVEKLVPSAVTSQAR"
     gene            1319..>1562
                     /locus_tag="PKZ53_22590"
     CDS             1319..>1562
                     /locus_tag="PKZ53_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43291.1"
                     /translation="MGIVLLLWISGALGLSSPFVCGARILVEASTTRFFPTVQTNSAV
                     TQSAVEPTSLSLADMIERELQAEQKQSFTVQFEANRY"
BASE COUNT          425 a          362 c          366 g          409 t
ORIGIN      
        1 cgaagccaat cagcgtcacc gccgggttga actcaaagaa catctccggg attttttcag
       61 tagtgcccac cgtctcaacc cgaatcgctg gatcacccaa accattgaac gccttggatt
      121 tgcccttctt gatggagaac tggcagccga agtgagacct gaaaccatca ttgcggttgc
      181 cctcaaaatt gcggctcagt ttgcggcaga aggttgcgct ggctggtgag ttgtttcaaa
      241 ttgcgattcc tggattttcc ccgagaccgg aaccggctcc attggctctc tacatccgct
      301 ggcaagctct ccggtatcgg aagaaaaaaa gcctgacccc ctttaaaaga tccagataga
      361 tgacagaaac gcaaaaacga tcataaaaga agttgtctgt tatcagtgtt ttatggaaaa
      421 ataactgaca ccggataaca acctgatctc tttttgccca cacgttgaaa tcgctcatgt
      481 ggaaaacctt ttaccgctgg ctcaaaacca aagaactcaa tattgtgtat cttggcccac
      541 ctctggctgg aaagcatgcc aatttgcgcc atctgcaagc gcttgcatta caagagtttg
      601 gcgtgaagtc gagcacgacc aatgaactaa ttttggaagg gagccagtta ttgccattgt
      661 tagggatgga ttcggcgttg gtagggatgc aaccgataga atttcgtatc cagacgattc
      721 caatcagcac ctatcgtgat ccggtacgtg atgacctgtt acggtcaatg gacgccgccg
      781 tcatggtttt tgattcacaa cggggacgga tggactcaaa tgccgcgatc aactggaatc
      841 tgaaaagctg gatgatcaat ctcaatcgag acatgtcagc tattccacag gttattcagt
      901 acaataaacg ggatttccca cggcttgaaa caattgaagt tctggaacag cggttaaacc
      961 ctcagcaatt gccgtatgtt gaggcttcgg cttccaaagg tgttggcgtg actgaaacct
     1021 tgcggatcct ggtagaaaaa ttagttcctt cggcggtcac ctctcaggct cgttaaagcc
     1081 agcagatcaa aatgtcacaa attctaaata aacttgccag atatctgacc gaagtggttt
     1141 acattcggtt tgatgaatgg atgggtatga tttacaccgt ctgagcaaat cgtcagtttc
     1201 cttgatgagt tgtcggtgtt tgatcatcag cagatgccgg aatcatcaat catttggtta
     1261 gaaccttaag atagaaaaca ggaaaaacat gaactgtcac cgacgatttt atcaccagtt
     1321 gggtattgtt ctgttgctgt ggattagtgg agcattgggt ttatcaagtc ctttcgtttg
     1381 cggtgcaaga atcctcgttg aagcatcaac cacccgattt tttcccaccg ttcaaaccaa
     1441 ttcagccgtg acccaatcag cagtagagcc aacatctttg tcgcttgctg atatgattga
     1501 gcgtgagctg caagccgaac agaaacagag ttttaccgtg caatttgaag ccaaccggta
     1561 tg
//
LOCUS       DAOQDO010001778         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90049_length_1562_cov_2.360319, whole genome shotgun sequence.
ACCESSION   DAOQDO010001778 DAOQDO010000000
VERSION     DAOQDO010001778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90049_length_1562_cov_2.360319"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1562
                     /locus_tag="PKZ53_22595"
     CDS             <1..>1562
                     /locus_tag="PKZ53_22595"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNJ43292.1"
                     /translation="KVEDKSGYHGYWAQDFLDIDPHVVSRTKLGGGEYPDTREGRLEH
                     YRDVIRLAHQKNIKVIQDVVMNHAGPVFYYDVNGNGQFDQDYTDKNNSEWIQPYLPDG
                     RHTNTKWADIGKWNMAKVGPAAGEIIKLSGSRPVKLNTSGIFQSFDAYTRKGYRDGSL
                     GKSDGEEVECDFFALRDFWTSKKNPVFDKLVDEFVEIYGFYIQELGVDGLRIDTVKHV
                     HHEFWDAFSERLRKKLPADVARRLILFGEVYSGNPRDLGKYTYRADGTATPNLDSLIH
                     FQTNYAIRDFLRVDGNGYKTPNSLAQMIEDAYVTKDNSGRPMYNPKAGLDGLSARQKM
                     VNFAENHDGLNRFRANGVSEQNNLLASALVLTLEGIPCLYYGTEANLIANSKIEGDSE
                     AGRLTFCKPNDLETLKQTRSNRSFVVLSSLMNYRKSLPALESGQISTLWVDSPESPDD
                     DGVFAFARFIQRGNSIATKDSVVVVVNANENEAKSAGTTQKRVKLVSRDGKPLISKGA
                     KLVRQPVAGQDL"
BASE COUNT          416 a          383 c          407 g          356 t
ORIGIN      
        1 caaaagttga ggacaaaagc ggatatcacg gatactgggc gcaggatttt cttgatattg
       61 acccgcacgt ggtcagtcga accaaacttg gcggtggcga atatcctgac acccgcgagg
      121 gccggctgga acactatcgg gatgtcatcc ggttggcgca tcaaaagaac atcaaggtga
      181 ttcaagatgt ggtgatgaat cacgccggcc cggtttttta ctatgacgtc aatggcaatg
      241 ggcagtttga tcaggactat actgacaaaa acaacagcga gtggattcaa ccctatctgc
      301 cggatggtcg ccataccaac accaaatggg ctgacatcgg aaaatggaac atggccaaag
      361 ttggcccggc ggctggcgaa atcatcaagt tatctggttc gcgacctgtc aaactcaaca
      421 cttctggtat ttttcagagt tttgatgcct acactcgtaa aggataccgc gatggcagtt
      481 tgggcaaatc agacggcgaa gaagtcgaat gcgatttttt tgccctccgc gatttctgga
      541 cgtcaaagaa aaatccagtc tttgacaaac ttgtagatga atttgttgaa atctacgggt
      601 tttatattca agagcttggc gtggatggac tccgcattga cacggtcaag cacgttcatc
      661 acgaattttg ggatgcgttt tcagaacggc ttcggaaaaa actcccggcg gatgttgcca
      721 gacgcctgat tctctttggt gaagtgtata gcggcaatcc ccgggacctt gggaaataca
      781 cctatcgagc cgatggcacc gccacaccga atctggattc cctgattcat tttcagacca
      841 attatgccat tcgcgatttt ttgcgggtgg atggaaacgg atacaaaaca ccaaattcgc
      901 tggcccagat gattgaagat gcctatgtca ccaaagacaa cagtggacgg ccaatgtaca
      961 acccgaaggc tggtctggat gggctttccg cccgccagaa aatggtcaat tttgccgaaa
     1021 atcacgatgg gctcaaccgg tttcgagcca atggcgtgag tgaacaaaac aatctgctcg
     1081 cttcggcgct ggtgctcacg ctcgaaggta ttccctgttt gtactacggc acagaagcca
     1141 acctgattgc caacagcaaa attgaaggcg acagcgaagc cgggcggtta acgttttgca
     1201 agccaaatga cctggaaacc ctcaagcaaa cccggagcaa ccggagcttt gtggtgcttt
     1261 cgtcgctgat gaactatcgc aagtcactgc cagcgcttga aagcggtcaa atttcgacgc
     1321 tttgggttga ttccccagaa agcccggatg atgacggagt atttgccttt gctcgcttca
     1381 tccagcgcgg aaactccatt gccaccaaag acagcgtcgt ggtggtcgtc aacgccaatg
     1441 aaaacgaagc caaatcggca ggcaccaccc aaaaacgagt caagctggtg tctcgtgatg
     1501 gcaaaccatt gatttcgaag ggggccaaac tggttcggca accagttgcc ggccaggatt
     1561 tg
//
LOCUS       DAOQDO010001779         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90075_length_1562_cov_2.036496, whole genome shotgun sequence.
ACCESSION   DAOQDO010001779 DAOQDO010000000
VERSION     DAOQDO010001779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90075_length_1562_cov_2.036496"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKZ53_22600"
     CDS             <1..262
                     /locus_tag="PKZ53_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43293.1"
                     /translation="LSYFYVDSVCEGVPFKGRRSDIEGELWLFRSTQVAISQVPGGWR
                     PNEDYSSEKTIVRYRFVLMPLFDFYVLYDKEDRVEMSIPAYE"
     gene            complement(513..>1562)
                     /locus_tag="PKZ53_22605"
     CDS             complement(513..>1562)
                     /locus_tag="PKZ53_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43294.1"
                     /translation="YAEAEKKFRRMIELDPDHPSGYVSLANCMWLRKLYTSRRLQTDL
                     YATKSFYAESEEKADPEFDKAFRETLKLAMTKSEGRLKKNKKDAVALYYLGSAHGLLA
                     AYEATVMRSFVAALRNGSRSVDYHEKTLEADPTYIDAKLTLGVYNYVVGSLPAPVKLL
                     ALMGGVKGSKKKGFELLEEARDHSRFVKDESRTMLIALYKRENRMEDALRELAILAEK
                     YPRNYLIQLEQATTLAKAGKAESSNQVFSTLLENQTASGIRDVINFQFAESLLVQGNQ
                     AEALKRYQRVIHTPKANGDLITLSHLKSGQLYDLANDRQKALGQYRLVSLRRNVFDSR
                     EQAKKYLKTPYSAQD"
BASE COUNT          358 a          368 c          395 g          441 t
ORIGIN      
        1 gctttcctat ttttatgtgg actcggtgtg tgagggagtt ccatttaaag gaagacggag
       61 tgatattgaa ggtgagttgt ggctattcag atcaacccaa gtagcaatta gtcaagtacc
      121 cggcggatgg aggccaaatg aagactattc atcagaaaaa acgatagtcc gctataggtt
      181 tgtgttgatg ccattatttg atttttacgt gctatacgat aaagaagatc gtgtggaaat
      241 gagtattcca gcttatgagt agcgttgcag tagaaacaat aggaacttca atggtatatc
      301 tgttatctgt tcattcaaga actgttatcg ggatctctta atgaatctga gtccaattgc
      361 ttgagcagtt tttccagata tgcctggatt tcagccgtcg tgagaccttt gaagcgctcc
      421 tcgggtggca atccttttcg cggagtgggg ccacttgacg ctgttttcat actgaaaagc
      481 ggtgtcaagc caccgcactc caaagcttga cttcaatctt gagcggaata tggtgttttc
      541 aggtactttt tggcctgttc ccgactgtca aaaacgttgc ggcggagcga taccagacga
      601 tactggccca gcgctttttg gcggtcattg gccaggtcgt acaactgtcc ggatttgaga
      661 tgtgacagtg tgatcaagtc tccattggct tttggcgtgt ggatcacccg ctggtatcgc
      721 ttgagcgctt cggcctgatt gccctggacc agcagcgact cggcaaattg aaaattaatt
      781 acatcgcgga tcccgcttgc ggtctgattt tccagcaagg tggaaaaaac ctgattgctg
      841 gactcggctt ttccagcttt ggccagcgtt gtcgcctgtt cgagctggat caaatagttg
      901 cgagggtatt tctcagccag gatggccagt tcgcggagcg cgtcttccat tcggttttca
      961 cgcttataga gcgcaatcaa catggtccgg gattcgtctt tgacaaagcg ggaatggtca
     1021 cgagcttctt ccagcaattc aaaccctttt ttcttcgatc ctttgacacc tcccatcagc
     1081 gccagcaatt tgactggcgc cggaagcgac ccaaccacat agttatagac cccgagcgtc
     1141 agcttggcat caatgtatgt agggtcggct tcgagcgttt tttcatggta atcaactgat
     1201 cttgaaccat ttcgcagtgc cgccacaaat gaccgcatca cggttgcctc gtaggcggcc
     1261 agcaaaccgt gagctgatcc aaggtaatag agcgccaccg catccttttt gttttttttc
     1321 agccggcctt cagatttggt catcgccagc tttaacgttt cgcgaaatgc cttgtcaaat
     1381 tcgggatcag ctttctcttc cgactcggcg taaaaagact ttgtggcata caggtcagtt
     1441 tgcaaccggc gcgaagtgta gagtttccgc agccacatac aattggccag gctgacatag
     1501 cctgacgggt gatctggatc cagttcaatc atgcggcgaa acttcttttc cgcttcggca
     1561 ta
//
LOCUS       DAOQDO010001780         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90123_length_1561_cov_3.337981, whole genome shotgun sequence.
ACCESSION   DAOQDO010001780 DAOQDO010000000
VERSION     DAOQDO010001780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90123_length_1561_cov_3.337981"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKZ53_22610"
     CDS             complement(<1..480)
                     /locus_tag="PKZ53_22610"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNJ43295.1"
                     /translation="MTLPMLRFFARSAALCLLILTASLTFMVKAEPFKDEHIEVELVS
                     EVESIQAGQPFWVALRMKTEAHWHTYWLNPGGPGLPTSLKWKLPAGFTAGPIQWPFPT
                     KIVTEGLVSYGYEEETFLLVRIQPPATLKPGTKIELAATADWLVCKEICLPGHADLKL
                     "
     gene            829..>1561
                     /locus_tag="PKZ53_22615"
     CDS             829..>1561
                     /locus_tag="PKZ53_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ43296.1"
                     /translation="MVWAQQSSDPNQASSDSKPKDPLGPEKFAGLKLRSVGPALTSGR
                     IIDFAVHPHNRAHYYAAAACGGVWKTTNSGTTWSPVFEKEGSYSIGTVVLDPKNPSVV
                     WVGTGEDNSQRSVAYGDGVYRSDDGGKSWKNLGLKASEHIGKIVIDPRNSNHVYVAAL
                     GPLWGPGGDRGLYKTTDNGKTWDKVLSISENTGVCDVIMDPTNPDVLFAAAYQRRRHV
                     WTLINGGPESAIYKSTDAGKTWNKLS"
BASE COUNT          392 a          403 c          407 g          359 t
ORIGIN      
        1 cagttttaaa tcagcatgac cgggcaggca gatttccttg cacaccagcc agtcagccgt
       61 agccgccagt tcaattttgg tacctggctt gagcgtagca gggggttgga tgcggaccag
      121 gaggaaggtt tcttcttcat agccgtagct gaccagtcct tcggtgacga tcttggttgg
      181 gaacggccac tgaattgggc cggcagtgaa tccggcgggc aacttccatt tgagggaggt
      241 cggaagccct ggtccaccgg ggttgagcca gtaggtgtgc cagtgcgctt cggtcttcat
      301 gcgcagggca acccaaaagg gctgtcccgc ctggatggat tccacttcgg aaaccagttc
      361 aacttcaata tgttcgtctt taaatggctc tgccttcacc ataaaagtca ggctggcagt
      421 cagtatcaat aggcacaggg ccgcagaacg ggcaaaaaag cgaagcatgg gtaatgtcat
      481 atgatcctga ttttgaaaaa agacggctgc cgccaaagag agacttttac acgacatgct
      541 gtctgaaacg gttaaatcaa atgaatgaac gttggatttg agatgagaaa ctgacgcaaa
      601 gtcaagtcaa gtgtgcgcta cgcaagtctt tacttcccta gattgctttc aaataattgc
      661 tacttaggtc cggtccgggc ataatcggtt cgcttacgtc aaaatttcaa ccgtcgccat
      721 tccactccta aaaatttaca acttcataaa aaggagaatc gggttttgaa gaaacttcgt
      781 gccttgttgt ctctcttttt gattgccgtg ctctcgccca cctttgcgat ggtttgggcc
      841 cagcaatctt ccgatccaaa ccaagcttcc tctgattcca aacccaaaga tccattaggc
      901 cctgaaaaat ttgctggtct caaattgcgc tcagttggtc cagcactcac gtcaggacgg
      961 atcattgact ttgcagttca tccccacaac cgagcgcact actatgctgc cgcagcctgc
     1021 ggcggggttt ggaaaaccac caattctggg acgacctggt cacctgtctt tgaaaaagaa
     1081 gggtcttatt caattggcac ggtggtgctt gaccccaaaa acccatccgt ggtgtgggtt
     1141 ggcactggtg aagacaacag ccagcgaagt gtggcctatg gcgatggcgt atatcgctcg
     1201 gatgacggtg gaaagtcgtg gaaaaacctc ggtctcaaag cgtctgagca catcggcaaa
     1261 atcgtgattg acccacgcaa cagcaaccac gtctatgtgg cggcactggg tccactctgg
     1321 ggcccaggcg gggaccgggg tctgtataaa accaccgaca atggcaagac ctgggacaaa
     1381 gtgctttcaa tcagcgaaaa taccggggtc tgcgacgtga taatggatcc aaccaacccg
     1441 gatgtcctct ttgccgcggc ctatcaacgc cggcgacacg tctggacact catcaacggt
     1501 ggcccggaaa gtgcaatcta taagtcaacc gacgccggca agacctggaa caaactctcg
     1561 t
//
LOCUS       DAOQDO010001781         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90132_length_1561_cov_2.980080, whole genome shotgun sequence.
ACCESSION   DAOQDO010001781 DAOQDO010000000
VERSION     DAOQDO010001781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90132_length_1561_cov_2.980080"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..619
                     /locus_tag="PKZ53_22620"
     CDS             347..619
                     /locus_tag="PKZ53_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43297.1"
                     /translation="MSLISMILLVLIAGICGAVGQSIAGYSRGGCLASVAVGFIGAML
                     GMWVARMVGMHELFAVRIGGQSFPIIWSIIGSALFVALISFISKQR"
     gene            1136..>1561
                     /locus_tag="PKZ53_22625"
     CDS             1136..>1561
                     /locus_tag="PKZ53_22625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ43298.1"
                     /translation="MVAATQELVHPHERTARKRYGVAIRLGFSFLMFGLMGYLRAQNI
                     LTAEHDLGAYALVSLGFILLAAYNFSIVLGSIGVPHVTAYLLIGILSGPYVFNLFSTD
                     VVKELGLVNNLALGLIAFIAGGELRLRILRERWKSISWMM"
BASE COUNT          387 a          346 c          362 g          466 t
ORIGIN      
        1 acaagcaggt aaaagcggtg tcaagccacc gcactccaat tattccagtt caattcgcca
       61 aaagtacaaa tcatcatctg taatcaccca gatattacaa acatacaccc tacatcgcag
      121 gcaacgttcc gagccgaaat cattcagtca ccgttggtag tcattttcca aaggtgaatg
      181 agttttcggc tcaatgttca tttaaactga cttaattctt tgattctatt tagcttattt
      241 tatttcatgt gctacggatt gctcttccag gcaacctgtg gtcctgccgg gtacaaccta
      301 tctgtggctg gcaacaatta taatttcaga tgaagggagc acgtcaatgt cacttatttc
      361 aatgattcta ctggttttga ttgccggaat ttgcggtgcc gtaggacaaa gcattgctgg
      421 atatagccgg ggcgggtgcc ttgcttctgt ggcggttggg tttattggcg caatgctcgg
      481 gatgtgggtg gcacgaatgg tcgggatgca cgaactcttt gccgttcgga ttggcggcca
      541 gagtttcccg attatatggt caattattgg ttcagccctg tttgtagccc tgattagttt
      601 tatttccaaa caacgataac caaagtttgt ggtcagtagt cagtagcgtg ctaagtttat
      661 ttgattgaac tatttgacta tcttctaatg gagagctagc aataccattt tgcaatgaaa
      721 cccttgtagt agaagatagt caagtaattc aatcaaatgg agttagcgaa ctactgacta
      781 cgggctactg actacaaact ggtataattc tttgtttgga aatgaaaaac gtgtcaagag
      841 ttccgccttc aggcggcccc agtctccctt cagatcactt tagggtacat acctcgcctg
      901 aaggcggaac gcttgactat gtgcttgatg gttgaaaaag ttttgggttg taagctggca
      961 tccttcctcg tttgaaaaca cccgagcaac gaatcgctcg cacagaagct ctgatttctg
     1021 taaacatttg atctcattcg tctttttcgt gattaaacct gtctttgaat tttcggcaga
     1081 tacatatcac aacgaaccaa aagctcaata atgaaaacaa aggaggcttc ccgcaatggt
     1141 tgctgcgacc caggaactgg tgcatccaca cgaacgaaca gcccgcaaac ggtatggtgt
     1201 ggccattcgg ctcggatttt cattcttaat gtttggcttg atggggtatt tacgagccca
     1261 aaacattctg acggcagaac acgacctggg agcctatgcc cttgtgtcac ttggctttat
     1321 cttactggct gcctataact tttcaattgt gttgggatca atcggcgtcc cacacgttac
     1381 cgcctatctg ctgattggga ttttgagcgg cccctatgtg ttcaatttgt tttccactga
     1441 tgtcgtcaaa gaacttggcc tggtcaataa cctggcactc gggctgattg cctttattgc
     1501 cgggggtgaa ctccggctgc ggattttgag ggaacgctgg aaatctattt cctggatgat
     1561 g
//
LOCUS       DAOQDO010001782         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90142_length_1561_cov_2.676627, whole genome shotgun sequence.
ACCESSION   DAOQDO010001782 DAOQDO010000000
VERSION     DAOQDO010001782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90142_length_1561_cov_2.676627"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..386
                     /locus_tag="PKZ53_22630"
     CDS             60..386
                     /locus_tag="PKZ53_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43299.1"
                     /translation="MSDQLRLAQFRNHLHTDFAVQISETQSMNLTLSEILVRQEKPTC
                     EEFSLHFHGPAEFLLAQQTLHLSHPVLGELDLFTVPIGRTQDGFTYEAIIYRFLDTET
                     STATTA"
     gene            483..980
                     /locus_tag="PKZ53_22635"
     CDS             483..980
                     /locus_tag="PKZ53_22635"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ43300.1"
                     /translation="MSSAPNATMPEIHLKPIESGDESFLFQIFAATRAEEMARVPWDD
                     SLKQAFLWSQFQAQSEQYRIKYPAGDFSIILLDNQPAGRLYLGRLDDQLRIIDLALLP
                     EFRRQGIAGKIMADLMAESRQKQKPIRVYVESFNPTLPWFFKLGFQKIDGDEVYYFLE
                     WQAQG"
     gene            1215..>1561
                     /locus_tag="PKZ53_22640"
     CDS             1215..>1561
                     /locus_tag="PKZ53_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43301.1"
                     /translation="MAFFNRESQSPSVLPVWKRQLVLCALWMIPGILIVAQVTFGPSA
                     RPRPHSWGWELFFQLLYWEPLALATPFVFYLSRQFPFEVPRRGRAFGFHLVAGIIFGF
                     VYALVLTPVSRWA"
BASE COUNT          356 a          434 c          385 g          386 t
ORIGIN      
        1 ctgaaccctg aaccctgaac cctgaaccct gaaccctgaa ccctgaaccc tgaaatccta
       61 tgtctgacca gctacgcctt gcccagtttc gcaaccatct tcacactgac tttgcggttc
      121 aaatcagtga aacccaatcc atgaacctga cactgagtga aattctggtt cggcaggaaa
      181 aacccacctg cgaagagttt tccctgcact ttcacggacc ggctgagttc ctgttggctc
      241 aacaaacgct tcatttgtcg catccggtgc ttggcgagct tgatctgttt acggttccaa
      301 ttggccgcac gcaggacggg tttacctacg aagccatcat ttaccggttt ttggatacgg
      361 aaaccagtac cgctacaact gcatgaatca gtctatcagg ctgaaaaaat cagggctgag
      421 ggctgaagaa agtgggttta ataccccaag ccctcagccc ttagcccgac ttcttcagcc
      481 cgatgtcttc agccccaaac gcaactatgc ccgaaatcca tctcaaaccc atcgaatcag
      541 gtgacgaatc ctttttgttt caaatttttg ccgccactcg ggccgaagaa atggcccgcg
      601 ttccctggga tgactcgctg aaacaggcat tcttgtggag ccagtttcaa gcgcagtctg
      661 aacagtaccg gattaaatat ccagccggag acttttccat catcctcctc gacaaccagc
      721 ctgctggtcg gctttacctt ggacgattgg acgatcagct tcgcatcatt gacctggctc
      781 tcctgccaga gtttcggagg caaggcatcg ctggaaaaat catggctgat ctgatggccg
      841 aaagccgcca gaagcaaaag ccgattcgag tctatgtcga aagctttaac ccgacgctgc
      901 cctggttttt caagctcggg tttcagaaga ttgacggcga cgaagtgtat tactttctgg
      961 aatggcaagc tcagggatga gaaaaccggg gctgaagaca tcgggctgag ggcttggggc
     1021 tgagaaaacc ggggctgaag acatcgggct gagggcttgg ggctgaagac tcgcaagctc
     1081 ggagcaattg aagtgatgag ggatgaaata aaaccaactt cttcagcccg tcttcttcag
     1141 cccaatatct tcagccccaa gcccgatgtc ttcagcccaa cttcttcagc cccaagccct
     1201 cagccctggt ttttatggca tttttcaatc gtgaatccca atcaccctct gtccttccag
     1261 tctggaaacg gcaactggtg ctgtgtgcgc tgtggatgat accggggatt ctgattgtgg
     1321 cgcaggtgac attcggtcct tcggcgcgcc cgcgacctca ttcctgggga tgggaattgt
     1381 tctttcaact tctgtactgg gagccgctgg cactggcaac gccatttgta ttctatctca
     1441 gccgacagtt cccttttgag gttccacgac gtggacgcgc cttcggattc catctggtgg
     1501 caggcattat ctttggattt gtgtatgccc tggtgttgac cccagtctca cgctgggcaa
     1561 t
//
LOCUS       DAOQDO010001783         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90319_length_1559_cov_3.097074, whole genome shotgun sequence.
ACCESSION   DAOQDO010001783 DAOQDO010000000
VERSION     DAOQDO010001783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90319_length_1559_cov_3.097074"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PKZ53_22645"
     CDS             complement(<1..1319)
                     /locus_tag="PKZ53_22645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43302.1"
                     /translation="MSQPSDPKKPPVGADETVQMGSAPLNPTAAAGNADVTQYVSSSK
                     PTRPNIDQTVAFHQSDHNAPTVMATITTGAVNKPNPMPVDVTIATGSTQAGPGSMDET
                     IATGSSLTGERFDSTLMGGSGVISNAETINATIAGGSTPTTPVGSATRAVQSKDGLSI
                     GDYFQKRYRIIKLLGAGGMGKVYQAHHEGLDKMVAIKFLIGNMNEDAEAIARFQREAQ
                     ATAKVSHPNAVQVIDTGVEDGICYIVMEFLEGESLRERIFRRGRIPLPETVHFAEKVC
                     YVLEYMHRKGITHRDLKPDNIFYQIQEDMETIKVLDFGIAKVHATATHAGSDLTREGM
                     MIGTPRYMSPEQCQGQGVDGRSDLYSLAVVVFEMLSGQVPYDADNPFSVALKQINSPI
                     PKLSEVAPHIPEAVSDVIYKAMAKAPKDRYPSVKDFSHALVKAANLQS"
BASE COUNT          337 a          419 c          434 g          369 t
ORIGIN      
        1 gattgcagat tggccgcctt taccagtgca tgggaaaagt ctttgaccga gggatagcgg
       61 tccttcggcg ctttggccat ggctttgtag atcacatccg aaacagcttc gggaatatgc
      121 ggcgccactt cagaaagctt cggaatcgga gaattgatct gtttgagcgc gacgctgaat
      181 ggattatcgg cgtcataggg cacctggcct gatagcattt caaacaccac aacggccagc
      241 gaatacaaat ctgaccggcc atccacgccc tggccctggc actgttcggg cgacatatag
      301 cgcggcgtac cgatcatcat gccttcgcgg gtcaggtcgc tcccggcgtg cgtggcggtg
      361 gcgtggactt tggcaatccc gaagtccagc accttgatcg tctccatgtc ctcctggatt
      421 tgatagaaga tattgtctgg cttcaaatcg cggtgggtga tgcctttgcg gtgcatgtac
      481 tccagcacgt agcacacctt ctcggcaaaa tggaccgttt ccggaagcgg aatccgccca
      541 cgtcggaaga ttcgttcccg gagcgattca ccttccagga attccatcac gatgtagcaa
      601 atcccatctt caacgccggt atcaatcacc tggacggcgt tggggtgcga gactttggcc
      661 gtggcctggg cttcgcgctg aaaccgggca atggcttcgg cgtcttcatt catattgccg
      721 atcaggaact tgatggctac catcttgtcc agaccttcgt gatgagcctg atagactttg
      781 cccatcccgc ccgcgccgag cagtttgata attcggtatc gtttctggaa ataatcgcca
      841 attgacagac cgtctttgga ttgaaccgct ctggttgctg atcccaccgg cgtggtcgga
      901 gtactgccgc cagctatcgt ggcattgatg gtctcagcat tgctgatgac gccagatcca
      961 cccatcagtg tcgaatcaaa ccgctcaccg gtcaggcttg atccagtggc aatggtctcg
     1021 tccatcgaac cgggcccggc ctgggttgat ccagtcgcaa tcgtgacatc aaccggcatt
     1081 gggttgggtt tattgaccgc accagtggta atggttgcca tcacagttgg ggcgttgtgg
     1141 tcggattgat gaaaggccac tgtctggtca atgttgggcc gggttggttt actggatgaa
     1201 acatattggg tgacatcggc attcccagcc gctgctgtcg ggttcagcgg ggccgacccc
     1261 atctggacag tttcatcagc ccccacgggt ggctttttcg gatctgacgg ttgtgacata
     1321 agacgtttct ccaaacactg accgacactt cacgctttga acagcacaaa gatcggcgga
     1381 ttggtggtgg cagtaaagta aggaagttaa tcgaacaacg atttcgggaa gctcaatgaa
     1441 tgaagctcaa aagtatagtg ccggcaccct gaaaaaccaa gctcaaaacc tgtcggcact
     1501 taggtgagtc aacagactcg gccctgaagg gccgagcttg agtcgtgtac ccacgacac
//
LOCUS       DAOQDO010001784         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90424_length_1558_cov_3.178310, whole genome shotgun sequence.
ACCESSION   DAOQDO010001784 DAOQDO010000000
VERSION     DAOQDO010001784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90424_length_1558_cov_3.178310"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    6..105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(182..>1558)
                     /gene="ftsY"
                     /locus_tag="PKZ53_22650"
     CDS             complement(182..>1558)
                     /gene="ftsY"
                     /locus_tag="PKZ53_22650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNJ43303.1"
                     /translation="KQSLFNRLMGREAEASKPSEESSAVQSTETLTSAGVAPAIATPA
                     SPEVIPSPVQFQPAAPPVSEPTTSPAAPVSWTTAPQPEPPVVVPPTPVPVETGEQSGK
                     SGFLSRFFGRGQSEAPTAPLPPPPAPVEVITPTLSPEEEAKQKFFYRFKNAVQKTREN
                     IVGRLDTLLRGQKVINAQVMDDLEEILLGSDIGVQTTLDLVEKIRKQVDRNQINNVDE
                     LKRLLKSELLAILTAPPPKRNFGGKSVASELELADDIRPYVMMMVGVNGVGKTTTIAK
                     LANLIKKEGNKVLICAADTFRAAASDQLAVWAQRTQVDLVQQKPGTDPAAVVFDSLQA
                     AKARDADVVIIDTAGRLHTKTNLMQELEKMTRIAKREVPQAPHEVLLVIDAVTGQNGL
                     EQARQFTRSVAVTGIVLTKLDGTAKGGIVVAISKELGIPIRYVGIGEQMDDLVVFSPE
                     DYVNGLFE"
BASE COUNT          301 a          378 c          404 g          375 t
ORIGIN      
        1 ctcgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgact gacgactgac
      121 gactgacgac tgacgactga cgactgacga ctgacgactg acgactggct attgaccacg
      181 atcattcaaa caacccattg acataatctt caggtgagaa taccaccagg tcgtccatct
      241 gttcgccaat gccaacatag cggattggaa ttcccagttc tttggaaatg gccaccacaa
      301 ttccgccttt ggccgttccg tccagtttgg ttagcacaat gccggtgacc gccacgctcc
      361 gggtaaattg acgagcctgc tccagtccgt tttggccagt gacagcgtca atgaccagca
      421 gcacttcgtg aggtgcctgg ggaacttcgc gtttggcgat gcgggtcatt ttttcaagtt
      481 cctgcatcag attggttttg gtatgcagtc gcccggctgt atcaatgatg accacatcgg
      541 catcgcgggc tttggctgcc tggagcgagt caaataccac ggctgccggg tccgttcccg
      601 gtttttgctg caccagatct acctgggtgc gttgagccca gacggcaagc tggtctgacg
      661 ccgctgcccg aaatgtgtca gccgcacaaa tcagcacttt attgccttct tttttgatga
      721 ggttggccag tttggcaatg gtggtggttt ttccgacgcc gttgacgcca accatcatca
      781 tgacatatgg ccgaatatcg tcagcgagtt caagttcaga ggcaaccgat ttaccgccaa
      841 aattgcgctt tggtggaggc gcggtgagaa tcgccagcaa ctctgatttt agcaaccgct
      901 ttaactcatc cacattgtta atctggttgc ggtctacctg tttgcgaatt ttttcaacca
      961 gatccagcgt ggtttgaacg ccaatgtcag accccagcag aatctcttcg agatcatcca
     1021 tgacctgagc gttgatcacc ttctgtccac gcaacagtgt gtcgagtcgt ccaacaatat
     1081 tttcacgggt tttttggact gcgtttttga agcgatagaa aaatttctgc ttggcttctt
     1141 cttcagggct cagggttggg gtgatgactt cgaccggtgc cggaggaggc gggagtggcg
     1201 ccgttggtgc ctctgactgt ccacgaccaa aaaaccggct gagaaaccct gattttccac
     1261 tctgttcacc tgtttcgact ggtactggag ttggcggaac aactacggga ggctctggtt
     1321 gcggcgccgt tgtccaggag actggagctg cgggtgaggt ggtgggctct gaaacaggcg
     1381 gtgcggctgg ctgaaattga accggagaag ggatgacttc tggtgatgcg ggagttgcga
     1441 ttgccggcgc cacacccgct gaggtcagcg tttcggtgct ttgaactgca gatgactcct
     1501 cagatggttt ggacgcttct gcttcgcgac ccatcaggcg attgaacaaa ctttgctt
//
LOCUS       DAOQDO010001785         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90441_length_1558_cov_2.702595, whole genome shotgun sequence.
ACCESSION   DAOQDO010001785 DAOQDO010000000
VERSION     DAOQDO010001785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90441_length_1558_cov_2.702595"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1434
                     /locus_tag="PKZ53_22655"
     CDS             217..1434
                     /locus_tag="PKZ53_22655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ43304.1"
                     /translation="MILWKRMQPGNASSKKQMVVCFLPVLFVTLVFVICATQTWGQTK
                     KPKTQPARGPAASPATSPATGTAQPTTVNTVEIQKVADGVYAAIRKEPPGLMFNGNCV
                     FIINDEDVVVVDTTITPSSAREVLAELKKLTSKPVKYVINTHWHDDHIIGNQVYREAF
                     PGVEFIGHASTLVDLPTVGASNRQQLQEGAPGFVEQIRATMNNKKNLVGADLTEEERI
                     SFTSDIEQVERYLSEIPKFQVVLPTITIQESLTLHRGSRTIEIRHLGRGHSGADLVVY
                     LPKERIAITGDLVVWPVPLVGSTSYPAEFAAALEKLVALQPTTIIPGHGQVMHDDTYA
                     RLLIRLLNSLKEQTAAAVARGETLEQARKSVNLDEFRKLIAGDSQLKNFIFRFYVSGP
                     GIEAAYQQASQKK"
BASE COUNT          431 a          395 c          378 g          354 t
ORIGIN      
        1 tcgaagggat gagggatgaa ggatgaggga tgaaagaaaa ccagttcttc aacccactgt
       61 cttcagcccg atgtcttcca ccccagggtt tttcagcccc atctacccaa acctccctaa
      121 atcggatata acccctctac caggctgact aaagattaaa aaacaacaaa gagcgtgttt
      181 atgtgggaaa cgtgacgctc aacgaggtaa ttcaagatga ttctctggaa aagaatgcag
      241 cccggtaatg caagctcaaa aaaacaaatg gtcgtttgtt ttttaccggt actgttcgta
      301 accctggtgt ttgtgatctg cgccacccaa acctggggac aaaccaaaaa gccaaaaacc
      361 caaccggccc gggggccagc cgcttcaccg gccacttcac cggccacagg aaccgcccaa
      421 ccaaccaccg tcaacacggt cgaaatccaa aaagttgccg atggtgtcta tgcggctatc
      481 cgcaaagagc caccaggact gatgtttaac ggcaactgtg tgtttattat caatgacgaa
      541 gatgtggtgg ttgtggatac caccatcacc cccagttcag ctcgtgaagt cctggctgaa
      601 ctcaaaaaat tgaccagtaa accagtcaaa tatgttatca acacccactg gcatgatgat
      661 catatcattg ggaatcaggt ctatcgcgag gcatttccag gggttgagtt tatcgggcat
      721 gccagtacgc tcgtggattt accaaccgtt ggggcctcaa accgccagca actgcaggaa
      781 ggtgcccctg ggtttgtcga gcaaatccgc gccacgatga acaacaaaaa aaatcttgtc
      841 ggcgctgacc tgaccgaaga agaacggatc agttttacca gcgatatcga acaggtcgaa
      901 cggtatttat ctgaaattcc aaaatttcag gttgtcttgc caactatcac gattcaggaa
      961 agtttgaccc tgcatcgagg cagtcgaacg attgaaatcc ggcatctcgg gcgcgggcac
     1021 agcggcgcgg atctggtggt ctatttgccc aaagaacgga tcgccatcac cggagacctg
     1081 gtggtctggc cagttccgct ggttgggtca acctcgtacc cggctgaatt tgccgccgcg
     1141 cttgaaaaac tggtggcctt gcagccgacc acaatcattc ccggccacgg ccaggtgatg
     1201 cacgatgaca cctatgcccg gcttttgatt cggctgttga actcactcaa agagcaaacc
     1261 gcagcggcgg ttgcccgtgg tgaaacgctg gagcaggccc gcaaaagcgt caatctggat
     1321 gaattcagaa aattgattgc cggagactca caactgaaga attttatctt caggttttat
     1381 gttagtggcc caggcattga agccgcctat caacaggctt cgcaaaagaa ataatttcag
     1441 tcagtagtca gtagtcaagt gttgggaatt gaaatttttg tcaagcgtat tctgttctgt
     1501 tcagacaagt tataccatcc tggaatagaa tccttggttc aaaaacagga attgattc
//
LOCUS       DAOQDO010001786         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90541_length_1557_cov_3.075899, whole genome shotgun sequence.
ACCESSION   DAOQDO010001786 DAOQDO010000000
VERSION     DAOQDO010001786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90541_length_1557_cov_3.075899"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKZ53_22660"
     CDS             complement(<1..882)
                     /locus_tag="PKZ53_22660"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNJ43305.1"
                     /translation="MVNQVPVVAVLMIVQGSLECLMGILLLILGPVMLAAPQHNGPSP
                     AGVGTFIFIFGVVIIIIAGLKIAAGVKGLKYRDRAFGIVTLLLGVVTFLTFYCAPTSL
                     ALMIYGLIVYLNQDVAKAFELGEQGYSPRDIQASFGQPFHQAGYSSPPVSGSGPGFPP
                     SYGSTAFGTHAGSTSPMPGYGVPSRSGAAPNSGSPGKGMAVASLILGLIGLFTAGLFL
                     VGAITGLILGIVSLTKSRKSAVYAGSSRGMAIAGIVLNSLAGLIAPLIAAIAIPNLLA
                     ARRAANTASAIQTLRTIH"
     gene            complement(1310..>1557)
                     /locus_tag="PKZ53_22665"
     CDS             complement(1310..>1557)
                     /locus_tag="PKZ53_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43306.1"
                     /translation="EDIDGWQLWLEPVTQLTFEQLNYLRKILARFGPGNQPENVLQVR
                     KQYCTGKRHLLLDNIWAGNYKEMSLHLRKLQIPHTFC"
BASE COUNT          447 a          382 c          385 g          343 t
ORIGIN      
        1 atggatggtg cgcaatgttt gaattgctga ggcggtatta gctgcccggc gagccgccaa
       61 caggtttgga atcgcaatgg ctgcaatcaa tggtgctatc aggccagcca gcgagttgag
      121 aacaattccg gcaatggcca tgccccggct actcccagcg taaacagcgc tttttcggga
      181 tttagtgagt gaaacaatcc ccagaatcag ccccgtaatt gccccaacca gaaacaaccc
      241 tgccgtaaag agtccgataa gacctagaat caaagaagca acggccattc cctttcctgg
      301 ggatccactg tttggtgctg cacctgacct cgatggtacc ccgtatccag gcatgggaga
      361 tgtgctgcct gcgtgcgtgc caaacgctgt actgccataa ctggggggga atcctgggcc
      421 agagcctgaa actggtggcg atgaataccc agcctgatga aatggttgac caaatgaagc
      481 ctggatgtcg cgtggagaat acccttgttc cccgagttca aaggctttgg ctacatcctg
      541 gttgagatag acaatcagtc cataaatcat gagcgccagg gaagttggag cacaataaaa
      601 cgtgaggaag gtcacaaccc ctaaaagcaa ggtcacaatt ccgaaagctc gatcccggta
      661 cttgagtcct ttaaccccag ccgcaatttt gagcccggcg atgataatga tgaccacacc
      721 gaagataaaa atgaaggtgc ccacaccggc tggcgaaggg ccattgtgtt ggggggcggc
      781 cagcatcaca ggccccagaa tgagcaggag aatccccatc aaacattcca aacttccctg
      841 gacgatcatc aaaactgcca cgactggcac ctggttgacc ataaaacctc cttgtgacca
      901 caagaaaaag gatcctgatg cctgggggta gggcatcccc attgatagca ccgtaacaat
      961 gaattctgaa tgacgaagaa ccacaaactg gcactgccag aatgcaatct gaagtgtgaa
     1021 attcaggaaa gagaattcaa aactcgctga gccaggaaat aaggtcccat cactaacctg
     1081 ccgtcagtct ataactgacg gcatactgat tggaatctct aagaaagttt cgggatgaac
     1141 tgaaaagatc tgaatctatt ggacaacagg ggagccacaa cccagatctt tgatgtggtg
     1201 aaacgtatgc ttatccatcc tgaattgcgt tcaacctttt atagcccagg atcggaaaaa
     1261 ccgcaaacct tatttaatgt cctgatggaa tgagctgaag tcatagaaat taacaaaaag
     1321 tgtgaggaat ctggagtttt ctcaaatgca ggctcatctc cttataattg cctgcccaga
     1381 tgttgtcgag cagcaagtgt cgtttcccag tacaatattg ttttcgaacc tgaagaacat
     1441 tctcaggctg gttccctggg ccaaaccggg caagaatctt gcgaagatag tttaattgtt
     1501 caaacgtcag ttgagtgacg ggttcaagcc agagttgcca gccgtcaatg tcttcga
//
LOCUS       DAOQDO010001787         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90641_length_1556_cov_3.356429, whole genome shotgun sequence.
ACCESSION   DAOQDO010001787 DAOQDO010000000
VERSION     DAOQDO010001787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90641_length_1556_cov_3.356429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1556
                     /locus_tag="PKZ53_22670"
     CDS             <1..>1556
                     /locus_tag="PKZ53_22670"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43307.1"
                     /translation="LYRKGEYARAEPLYLRALAIYEKASGSDNLEVVNILNNLAALSY
                     QKGDYARAEPLFLRSLAISEKILGANHPAISNVLNNLAALSYQKGDYARAEPLMVRAL
                     AIAETALGPDHPDVAQSLSSLASLYQIKGDYTNAEPLLVRALAIREKSLGADHLSVAN
                     SLNNLAGIYQGKGEFAKAEPLYVRALAISEKVLGPSHPIIASLLNNLAVLYLSNREYT
                     KAEPLYLRALAIREKTLGAGHPETAQSLNNLATFCHKGGDYAKAGLLLVRALAITEKT
                     LGAEHREVAGSLNSLAAVSEARGEIPQAIRYRIRSNDATERDLVRNLVAGSEFQKSTY
                     LKQTASYTHSTLSLHLQSAPHDLEAAQAGFTVILRRKGRALDAMTDALAILRNQQRPE
                     TQKLLDDYTSLVGQISVLTLRGPGTKKPEVHLAEIRSLEDQKEKLENDISRRSGEFKA
                     QTTPITLEAVQKLIPTNAALVEYVVYRPFDAKTGDFGKPQYAAYVLQGIGDRGQGIGI
                     EKQGSGFRVP"
BASE COUNT          402 a          390 c          438 g          326 t
ORIGIN      
        1 cttatatcgc aaaggcgagt atgcacgggc ggagccgttg tatctaaggg cattggccat
       61 ctatgagaag gcatcaggtt ctgacaacct ggaggtcgtc aatatcctca ataacctggc
      121 ggcgctctct tatcaaaaag gtgactatgc acgagccgaa ccgctctttt tgaggtcgct
      181 ggcgatttct gaaaagatat taggcgcgaa ccacccagct atttccaacg tccttaataa
      241 tttggcggcg ctctcttatc aaaaaggtga ctatgcgcga gccgaaccgc tgatggtacg
      301 ggcactggca atcgccgaga cggcattggg acccgatcac ccagatgtcg cacagagtct
      361 cagcagtctg gcttcgctct accagatcaa aggggattac acaaacgcgg agccgctcct
      421 ggtaagggca ttggcgatca gggaaaaatc cctgggtgct gatcatctga gtgttgccaa
      481 cagtttgaac aatctggctg gaatctacca ggggaaaggt gagttcgcca aagccgagcc
      541 gctctatgtg agagcactgg caatctctga gaaggtactg gggccttctc atccaattat
      601 tgccagcctg ctcaacaacc tggcggtgct ttacctgagc aatcgtgaat acacgaaggc
      661 cgaaccgctg tacctgagag cactggcgat cagagaaaag acattggggg caggtcaccc
      721 agagacagcc cagagcctca acaatctggc gaccttctgt cacaagggag gggattacgc
      781 gaaagctgga ctgctgctgg tgagggcgct ggcgatcacg gaaaagacat taggggctga
      841 acaccgggag gtcgccggta gtctgaatag cctggcggcg gtttctgaag ccagaggaga
      901 aatacctcag gcgattcggt atcggatacg tagcaacgac gccaccgagc gtgatctggt
      961 ccgcaatctg gttgctgggt ccgagttcca gaaatcaact tacttgaaac aaacggcgag
     1021 ctatacgcac tcgactcttt ccttgcacct gcagagcgcg ccgcacgatt tggaagccgc
     1081 ccaggctggc ttcactgtaa ttttgcgccg taagggacgg gcgctggatg cgatgaccga
     1141 tgctctggca attttacgga atcaacaacg tcccgaaacc caaaaactcc ttgatgacta
     1201 tacaagcctg gtcggtcaaa tttcggttct gacgttgcgt ggtccaggca caaaaaagcc
     1261 tgaagttcat ttggctgaga ttcggtcact tgaggaccag aaggaaaaac tagaaaacga
     1321 catcagccgg cgctctggag aatttaaagc ccagaccacc ccgattaccc tggaagccgt
     1381 tcaaaaacta attcccacca atgcagcact ggtggaatat gtcgtgtatc ggccctttga
     1441 tgcgaagact ggagactttg gaaaaccaca gtatgcggct tatgttttac aggggatagg
     1501 ggacagggga caggggatag gaattgaaaa acagggttcc gggttccggg ttccgg
//
LOCUS       DAOQDO010001788         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90649_length_1556_cov_3.051965, whole genome shotgun sequence.
ACCESSION   DAOQDO010001788 DAOQDO010000000
VERSION     DAOQDO010001788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90649_length_1556_cov_3.051965"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..636)
                     /locus_tag="PKZ53_22675"
     CDS             complement(163..636)
                     /locus_tag="PKZ53_22675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43308.1"
                     /translation="MKQLTHFSDVPLHLQRLTVSVYFGWIVTCIVFFVLGLAIGKLST
                     SRFESAVQVQSTRSDKPTPGQTNEERLAQTAAATAQCTVLVTVVSNASEANQVVADLR
                     RKGFTSAYITLPAEGSPNPAYTVKVGFYNLPTANQVAEELQRDLGFKNARVITNN"
     gene            1040..1378
                     /gene="hfq"
                     /locus_tag="PKZ53_22680"
     CDS             1040..1378
                     /gene="hfq"
                     /locus_tag="PKZ53_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNJ43309.1"
                     /translation="MDKAPPPQNLQDSFLNQARRDRVTVAIYLMSGVKLTGRIRGFDK
                     YSVILESGNQEQMIFKHAISTVSMLRSSGGRPYEGGGSERGEYRENTGRGHAGAAGHS
                     GSNDSDKDEG"
     gene            1375..>1556
                     /locus_tag="PKZ53_22685"
     CDS             1375..>1556
                     /locus_tag="PKZ53_22685"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNJ43310.1"
                     /translation="MTSGLFITFEGIDGCGKTTQWRLLTRYLESNGINLLATREPGGT
                     ALGVQIRKLVLEKTVST"
BASE COUNT          408 a          344 c          404 g          400 t
ORIGIN      
        1 caatcagttg catcaatcgg agtgtgccct gccgggtcag gttgatcggt ttgaaggaaa
       61 agagaaacag gtgatgtgtc ataaaggcga aatagtgaaa taaggatttg tcagcaccaa
      121 cacttgagag ataaaaggtt tcatctcttt cgcgctttca ctctaattat tggtaatcac
      181 tcgggcattc ttaaaaccaa gatctcgttg gagttcttcg gcgacctgat tggccgtcgg
      241 gaggttataa aaaccaactt tcacggtata ggccgggttg ggtgaccctt cggcaggcaa
      301 agtgatatag gcgctggtga atccttttcg ccgtaagtca gccaccactt gattggcttc
      361 gcttgcattg ctgaccacag tcaccaaaac cgtacattga gccgtagccg ctgccgtttg
      421 ggccagccgt tcctcgttgg tttgacccgg cgttggttta tcagagcgtg ttgattgcac
      481 ctgaaccgct gattcaaagc gtgatgttga gagcttgcca atggccaggc caagcacaaa
      541 aaacacaatg caggtcacaa tccagccaaa atagactgaa accgtaagtc gctggaggtg
      601 gagcggtaca tctgaaaaat gggtaagttg cttcattgga aaaacttgaa cgaaatgatg
      661 aggtcaactc gcaggtgatt gctgaaaaga aaggctacta tacggcggaa tgggcgtgag
      721 tttcaacacg agaaacgggc tccggctgta agttcagatt gtgaatcaat tccgagtttg
      781 caaacaagtc ggctttggag ttgcaagaag gaatttttgt atggtagggt gtgtgccgag
      841 ctttgtccgt ccatcatcgc cttgaagctt cccaaatttt gttatcaacc tgtcattttt
      901 tcttctcatt ttctagagtt caagaactcg ttcaggccgc tgtgtcatag actcgcaacg
      961 ctgtttcttg aaatgctctc ttctgatttc cccgccaatt ctttgagttg gcaatgctcc
     1021 gtgaaagagg ataaataaca tggataaagc acctcctcca cagaacctgc aagatagttt
     1081 tctcaaccag gctcgacgcg accgcgttac cgttgccatt tacctgatga gtggcgtgaa
     1141 gttaaccgga cggattcgag gatttgataa atactccgtc attcttgaat ctggcaatca
     1201 agaacaaatg atttttaaac acgcaatttc aactgtatca atgctccgtt catctggtgg
     1261 gcgcccatat gaaggagggg gaagcgagcg tggcgagtat cgtgaaaata ctggtcgtgg
     1321 gcatgctgga gctgccgggc attccggatc aaatgacagt gataaagacg aaggatgacc
     1381 agcggactct ttattacgtt tgaaggcatt gatggttgcg ggaaaacaac ccaatggcgg
     1441 ttgttgaccc gctacctgga atcaaacggt atcaatctgc ttgcgacccg tgaacctggc
     1501 gggacggcgc tcggcgtcca gattcgaaag ctcgtgctgg aaaaaacggt atcaac
//
LOCUS       DAOQDO010001789         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90669_length_1556_cov_2.564957, whole genome shotgun sequence.
ACCESSION   DAOQDO010001789 DAOQDO010000000
VERSION     DAOQDO010001789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90669_length_1556_cov_2.564957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1377
                     /locus_tag="PKZ53_22690"
     CDS             304..1377
                     /locus_tag="PKZ53_22690"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNJ43311.1"
                     /translation="MFPFSLPNTVPPVLVEITRGGLTESQIRGMIAVVEPTGRLVASI
                     GQIDFPCYLRSVAKPFQSTSLFLTGVISTYHITPRELAVMTSSHNGERFHVACVREIL
                     ARTAIPEACLRCGVHLPFNEASIAAMNGQNPQPIHNNCSGKHAGMLTAARLMGAPLET
                     YEETDHPVQVLNREQVAYFTDVPASELSPASDGCTVPTWPIPLRNLALGYARLAAPEN
                     LPLLADVSRRDQTIDASRQIVQAMMQHPEMVGGTGRIDTALMQAVPGKLVSKVGAEGV
                     HAFAVLPCDSFPTGLGMAIKLEDGDNFRARHPVVIAVLEQLGVVSSDQAHRLRALYAP
                     PIRNCREEIVGEPRVVFSLKNQG"
BASE COUNT          369 a          432 c          402 g          353 t
ORIGIN      
        1 ggaaaaaaat aacgagtgga aacggcaaca aggcgacagg accaaaaaat ctgaagtgag
       61 gctggatttt actgaatttc ccggctacag gtctagcagg atattgccgt ctgaggtctg
      121 aatatttcgg gtcaggcggg taatgtatct gattctccta taaaaacaaa tcgaaaaatt
      181 attcacgccg cgatagagtg cggagcgcag cggctttgag gagtgcggtg gcttgacacc
      241 gctttctcat ccgacccaaa aacgaaagcg gtgtcaagcc accgcactcc acacaacatt
      301 cgtatgtttc cattttctct tccaaacaca gtgccaccag tactggttga aatcactcgt
      361 ggcggcctga ccgaatccca gatccggggc atgattgccg tagttgaacc aaccggacgg
      421 ctggtcgcca gcattggtca gattgacttt ccctgctatc tgcgctcagt ggccaaacct
      481 tttcaatcaa cgtcactttt tctcaccggg gtcatttcca cctatcacat cacccctcgc
      541 gaactggcag taatgacttc gtcacacaac ggcgagcgct ttcatgtggc ctgtgtcagg
      601 gaaattctgg ctcgaaccgc tatcccagaa gcctgtttgc gctgtggggt acatctgccc
      661 tttaatgaag cctcaatcgc ggcaatgaac gggcagaacc cccaaccgat ccataataac
      721 tgttcaggaa aacacgctgg aatgctcacg gcggctcgtt tgatgggcgc tccgcttgaa
      781 acctatgagg aaacagacca tccggttcaa gttctcaacc gtgagcaagt ggcgtatttt
      841 actgatgttc cagcttcaga actctctccg gcgagcgatg gatgcaccgt tccgacctgg
      901 ccgattccac tccgtaatct ggcacttggc tatgcccggc tggccgcacc agagaacctg
      961 ccactcctgg ctgacgtcag tcgccgcgac cagaccattg atgccagccg ccagattgtc
     1021 caggcgatga tgcagcatcc agagatggtt ggtggaactg gccgcattga tacagcgctg
     1081 atgcaggcag tccccggaaa actggtctca aaagttgggg ccgaaggcgt gcatgccttt
     1141 gcggtcctcc cctgtgattc attcccaact ggactgggca tggccattaa acttgaagat
     1201 ggtgataact ttcgggcgcg gcatccggtc gtgatcgcag tgcttgaaca attgggggtg
     1261 gtgtccagcg atcaggctca ccggctgagg gctctatatg cccctccaat tcgcaactgc
     1321 cgcgaagaaa tcgtgggcga accacgggtt gtgttttcac tgaaaaacca gggctgagaa
     1381 aaccagggct gagggctgaa gatattgggc tgggggctga agaaaacggg tttcataccc
     1441 cgagccgacg tcttcagccc cgagcttgcg agccttcagc ccgacgtctt cagccctaaa
     1501 cctaacctat ctcatttcag gaacactctt tatgcaacga atcttcctca tccttg
//
LOCUS       DAOQDO010001790         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90761_length_1555_cov_2.828000, whole genome shotgun sequence.
ACCESSION   DAOQDO010001790 DAOQDO010000000
VERSION     DAOQDO010001790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90761_length_1555_cov_2.828000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PKZ53_22695"
     CDS             complement(<1..1138)
                     /locus_tag="PKZ53_22695"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNJ43312.1"
                     /translation="MTSMKTALNPKGTKRIVTWSLALWMLFISGIAIQAQGPQRQELA
                     LTQLPGTVATINGDQFRLSTGFSSKLAESSGLPGHGGQVLSAQFNQHPAFTKKSEDGL
                     NSIFRSKITAINGNTIECEGGVTASIASASLYHSAFRTTSRSELTVGTHVWLNGKFVT
                     SPQGNAYLDAGYCLIETDTTMVIRGIVQKVDAKNQTLTILNQTVKVSPQADIQNVINP
                     KKLKLSSFKPGHDVFIGGEVINGTLVANSLVLRDKPKDPDDGEGYLIGTIRELNNDVA
                     RFDGGFVFDVSKFNAANAPLLNLQAGRRMEVDFNFDDVPTTAAPIKVENGVLPQDLDH
                     RLFGLVQDVNAATSMVTVFNQSLMIPDGVFLFFPPLPQSVSQLTA"
     gene            complement(1314..>1555)
                     /locus_tag="PKZ53_22700"
     CDS             complement(1314..>1555)
                     /locus_tag="PKZ53_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43313.1"
                     /translation="MALIGSQLGQVNRPEGVTVKFFQTGAFAGNLLLVVGDTMNNRIQ
                     GRFLPTGQSGQWSLIGAPNGSGTTVGRFRNPSKLQ"
BASE COUNT          378 a          379 c          404 g          394 t
ORIGIN      
        1 ccgccgttaa ctggctgact gattgtggca gtggtggaaa gaacaaaaac acaccgtctg
       61 gaatcatcaa actctggttg aacaccgtga ccatgctggt ggcggcgttg acatcctgaa
      121 ccagcccaaa cagtcggtgg tccagatctt gcggaagcac tccgttttcg accttaatcg
      181 gcgcggctgt ggttggaaca tcatcaaaat taaagtctac ttccatccga cgtccagcct
      241 gcaggttcaa aagcggcgcg ttggcagcat tgaattttga aacatcaaaa acaaacccac
      301 catcaaatcg ggcaacatcg ttatttaatt cccgaatcgt tccaatcaga tagccttcgc
      361 catcatctgg atctttcggt ttgtcgcgca acaccaggct gtttgcgacc agcgtgccgt
      421 ttatgacttc gcctccgata aaaacatcgt ggccgggttt gaagctcgac agtttcagtt
      481 tcttcggatt gatgacattt tggatgtcgg cttgaggact gactttgacg gtttgattca
      541 agatcgtgag ggtctgattt ttggcatcaa ctttctgcac aattccccga atcaccatcg
      601 tggtatcggt ttcaatcaaa caatatccgg catcaaggta tgcattaccc tgtggcgagg
      661 taacaaactt cccattgagc cagacgtgag taccgaccgt gagttcagat cgtgaggtgg
      721 tccgaaaggc tgagtgataa agtgaagctg aggcaatact ggcggtgaca ccgccttcac
      781 attcaatcgt gttgccattg atggcggtga ttttactgcg gaagatcgaa ttgagtccat
      841 cctcgctttt tttggtaaag gcgggatgtt ggttgaattg agctgaaagg acctggcctc
      901 cgtgaccagg aagccctgaa ctttcagcca gtttggatga aaaaccagtg ctcaatcgaa
      961 attgatcgcc attgattgtg gctacggttc caggcaattg tgtcagggcg agttcttgcc
     1021 gttgcggccc ttgtgcctga atggcaatac cggaaatgaa aagcatccag agcgccaggc
     1081 tccaggtcac aatgcgtttc gtgcccttcg ggttgagggc cgttttcatt gatgtcataa
     1141 agatcctcca aaatcgctgt ttaaccgggg atcacacctg agtgtgtccc ctatttcgaa
     1201 acgcgcagcc aaaaacgcaa aattgtcagg caaggcggga gaattttttg gaggatgcag
     1261 aatctttatc gtggatgcag aatctttatc gtggaaaagc cgggttcgtc cggtcattga
     1321 agtttcgatg ggttgcggaa acgacccacc gttgtgccag agccgtttgg cgcgccaatc
     1381 aaactccatt gtccagattg tccagtcggg agaaaccggc cctggatgcg attgttcatt
     1441 gtgtctccga ccaccagcag gagattgccg gcaaaggcac cggtctggaa aaacttgact
     1501 gtcacgcctt cgggacgatt gacctgaccg agttgtgacc caatcaacgc catcg
//
LOCUS       DAOQDO010001791         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90771_length_1555_cov_2.596000, whole genome shotgun sequence.
ACCESSION   DAOQDO010001791 DAOQDO010000000
VERSION     DAOQDO010001791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90771_length_1555_cov_2.596000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..652)
                     /locus_tag="PKZ53_22705"
     CDS             complement(119..652)
                     /locus_tag="PKZ53_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017377365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ43314.1"
                     /translation="MAIQAIKDQLGDFAKDTKLNLSSVLTEEGAPDLKKNQIFGIALA
                     SAYATKDRQLVAEILSEAKEVLSEAEINAAKAAATIMGMNNVYYRFQHLVSDKDYTTM
                     PARLRMNVIGNPGVKKLDFELYSLAVSAINGCGRCIDAHADSIVKEGLSKTGVQSTVR
                     IASVINAAAQGLFINSL"
     gene            complement(804..1343)
                     /locus_tag="PKZ53_22710"
     CDS             complement(804..1343)
                     /locus_tag="PKZ53_22710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005772008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ43315.1"
                     /translation="MLGVGDLFPKFQVTATVSNDIKNAFTEINNDTYQGKWLVVFFWP
                     KDFTFVCPTEIAAFGELNQEFADRDAQVLGASTDSEFVHLAWRQHKEELNNLPFPMLA
                     DVKRDLTTGLGILDKNAGVAQRATFIVDPQGVIRFTMVTDLSVGRNPKEVLRVLDALQ
                     TDELCPCNWQKGEETIVVA"
BASE COUNT          379 a          354 c          400 g          422 t
ORIGIN      
        1 cccagagccc atcccaactc agaaaaagcg ggaacgataa aggaaaaaaa taaacggagg
       61 atgtcgttgg cgacgtcctc cgtttaacag ggagcgatgc aattgggtgc gctgaagctt
      121 acagagaatt gataaaaagt ccttgtgcgg cagcgttgat caccgaagcg atccgaaccg
      181 tagattgaac gccggttttg gaaagtcctt ctttcacaat cgagtccgcg tgggcgtcaa
      241 tgcagcgtcc gcagccatta atagctgaaa cagccagcga atataattcg aaatccagtt
      301 ttttcacgcc gggattgcca atgacattca tccgcagccg ggcgggcatg gtggtgtagt
      361 ctttatctga gaccaggtgc tgaaaccggt aatagacatt gttcatgccc ataattgtcg
      421 cagcggcttt ggcggcgttg atttccgctt ctgacaaaac ttctttggct tctgacagga
      481 tttccgcgac cagttgacga tctttggtgg catatgccga agccagggca atgccgaaaa
      541 tctgattttt cttgaggtcg ggcgcgcctt cttcggtgag aacgctcgac aggttgagtt
      601 ttgtatcctt ggcaaaatcg ccgagttgat ccttgatagc ctgaatcgcc atggtttgct
      661 ccttatgaag taaccgggga ctgatgggaa aggaaactgg gatatttgat aagccttccc
      721 aattttcctt tcccggtttt ttctctctga acgctgaagt tgttgaaaat caatgagttg
      781 aagtattcaa cccgtgatgg ttcttaggca acgacgatgg tttcttcacc tttttgccag
      841 ttgcaggggc agagttcgtc ggtttgcagc gcatcgagga cgcgcaagac ttctttcgga
      901 ttgcggccta cgctcaggtc cgtgaccatc gtgaagcgga tcacgccttg tgggtcaaca
      961 atgaacgttg cacgttgggc gacacccgca tttttatcca aaattcccag accagtggtc
     1021 aaatcacgtt tcacatcagc cagcatcggg aacggcaggt tgtttaattc ttccttgtgc
     1081 tgccgccagg ccaggtggac aaattctgaa tcggtgctgg cgccaagcac ctgggcatca
     1141 cggtcggcaa attcctgatt caattcgcca aacgcggcaa tttcagttgg gcagacaaaa
     1201 gtgaaatctt tgggccagaa gaacaccacc agccatttgc cctggtacgt gtcgttgttg
     1261 atttcggtga atgcgttttt gatgtcgttt gaaaccgtgg ccgtgacttg aaacttcggg
     1321 aaaagatctc ctacgcctaa cattgtttat cctccaaaaa aggttggttt gttgtttatg
     1381 aatgttcgta tggttgaaaa cttgcttgat ggcggtactc tagggaaaga gtttcgataa
     1441 gtaaaatata ttgtttttat caaaatgata atccagaatt atcattaaaa tttgccgcgg
     1501 tggaatgcgg cccacttgac gccgcttttg gtggcgctta tttcaccaca aacgg
//
LOCUS       DAOQDO010001792         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90857_length_1554_cov_3.580387, whole genome shotgun sequence.
ACCESSION   DAOQDO010001792 DAOQDO010000000
VERSION     DAOQDO010001792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90857_length_1554_cov_3.580387"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="PKZ53_22715"
     CDS             <1..1447
                     /locus_tag="PKZ53_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43316.1"
                     /translation="ALQASRAKSTFLANMSHELRTPLNAVLGFAQLMERDQKLLAEHR
                     ENLAVILRSGEHLLELINDVLSISKIEAGKLSLVLEPFDLKLLLKGIREMVQVRAQSK
                     GLRLIFDLDPALPIYVKGDEGKLRQVLINLLGNAVKFTEVGGVALRVQVNGDLLEFEV
                     EDTGQGIAEDELNNLFQPFVQAASGRKSAEGTGLGLAISRTFIQLMGGDIRVRSTPGK
                     GSIFTFELPLPAVNDAIKPTQSRRVRGITFGQPTYRILVVDDVAENRLLLNKLLSPIG
                     FQVREAVNGREACEVWSGWHPHLIWMDLRMPVMNGYAATRFIREAENKLHFDKLPAPD
                     AGGGKAGSRSLRRPHTVIIALTASAFEHDRKGILEAGCDDFVTKPYREQTIFDKLTEH
                     LGIQFIVENEAPVYVPPSETALTTERFAQLPPEWIAELNRALTLGDLMGARSIIDQIS
                     ELDPDLADELRQKVKEYQFDEVFHLIERMQV"
BASE COUNT          343 a          378 c          448 g          385 t
ORIGIN      
        1 agccctgcag gccagccgcg ccaagagtac tttcctggcc aatatgagcc acgaacttcg
       61 gacgccactc aatgcggtgc tggggtttgc ccagttgatg gaacgtgatc aaaaactgct
      121 ggctgaacac cgcgagaatc tggcggtcat tttacgcagt ggcgagcatt tgcttgaact
      181 catcaacgac gtgctctcga tttccaaaat tgaagctgga aagctctcgc tggtgctaga
      241 gccattcgat ttaaagctcc tgctcaaagg aatccgtgaa atggtgcagg ttcgggcaca
      301 gtccaagggg ctacggttaa tttttgattt ggatccggcg ttgccaattt atgtcaaagg
      361 agatgaaggc aaactgcgtc aggttctcat caatttactt ggcaatgcgg tgaaatttac
      421 cgaagtcggc ggcgttgccc tgcgggttca ggtgaatggt gacttgcttg agtttgaagt
      481 tgaggatacc gggcagggaa ttgctgaaga cgaactcaac aatctgtttc agccctttgt
      541 tcaggccgcc agtgggcgaa aatctgctga aggcacgggc ctggggctgg ccatcagtcg
      601 aacgtttatt cagttgatgg gcggtgacat tcgggtgcgc agcacacctg gaaagggttc
      661 gatctttacg tttgaactcc cgttgccggc ggtcaacgat gctattaaac ctacccaatc
      721 ccgacgggtt cggggcatta catttggtca gcccacctat cgcattctgg tggtggatga
      781 tgtggcggaa aaccggttgc tccttaacaa gttgctttct ccgattgggt ttcaggtccg
      841 tgaagccgtc aacgggcgcg aagcctgcga agtctggtcc ggttggcatc cacatttgat
      901 ctggatggat ctacgcatgc cggttatgaa cggctatgcc gccacgcgtt ttattcgcga
      961 agctgaaaac aaactgcact ttgataagtt gcctgctcct gacgccggcg gcggaaaagc
     1021 cggtagtcgg tcactccgcc gcccgcatac ggtgattatt gccttgacgg cgagtgcttt
     1081 tgagcatgac cggaagggaa ttttagaagc cgggtgtgac gactttgtaa ccaagcccta
     1141 tcgcgaacag acgattttcg acaaattgac cgaacatctg gggattcaat ttatcgttga
     1201 gaacgaagcg ccggtgtatg ttccaccttc cgaaacggca ctgaccactg agcgctttgc
     1261 ccagcttcca cccgaatgga ttgcggagtt gaatcgggcc ctgacgcttg gtgatttaat
     1321 gggcgcccgg agcatcattg atcaaatcag tgagcttgat ccggacttag ccgatgagct
     1381 ccgccaaaaa gtcaaagaat accagtttga tgaagtcttt catttgattg aacgaatgca
     1441 ggtgtgaact gtggagtgcg gtggcttgac accgctttca gattcggcgg cttgacgcct
     1501 gtggagtgcg gcggctcgac gccgctttcg tattcggcgg cttgacgcct gtgg
//
LOCUS       DAOQDO010001793         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90864_length_1554_cov_3.410941, whole genome shotgun sequence.
ACCESSION   DAOQDO010001793 DAOQDO010000000
VERSION     DAOQDO010001793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90864_length_1554_cov_3.410941"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="PKZ53_22720"
     CDS             <1..>1554
                     /locus_tag="PKZ53_22720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ43317.1"
                     /translation="LVSDDVSFGACLTRRDGGTWFGTNDGVSRYKAAKEISLPVPPRL
                     ALSTVRIQEKTVEIIPKDNQVSLQLPPLSYYENTLTFEFAAMSFIDPEAVRYQYRLEG
                     FDHDWSAPVSDRFVRFINLPPGNYRFFIRAISASGVWTDPQNIQVTIQLPFWQTWWFR
                     FLVLLLVTTTIGVVYTANIYRIKRRQEEKLAVLRQIQEQRIKSLRELLESIRVINSNL
                     ELENVLQNIAEESARLVDGEPGGIGLVENGKVCFRRLWRQDHWESSPLEFHLGDGIAG
                     KVAQNARPMIVNEPVQSTDVIYPELIEEYFVHGWMDVPIVARSGQVVGVLDVRRKSGR
                     PPFTESDCHLIEALAHQAAVAIENAGLYGELEEKNLMVVESMHELEKLYKQEREVTRA
                     LQDIDRMKTNFITVMSHEMRTPLTVIKGYNEAILDQYFGPLTPIQEKSLQTCQRMVER
                     LVCSFNDILEMLKINEGHVELRYERVNIRTLVDEVLSDLANFIENRHQHITLDSPAEF
                     HIEIDSDKI"
BASE COUNT          392 a          367 c          421 g          374 t
ORIGIN      
        1 gtctggtcag cgatgatgtc agttttggag cctgtttgac ccgtcgcgat ggcggaacct
       61 ggtttggcac caacgatggt gtttcacgat ataaagccgc caaggaaatt tcgctcccgg
      121 ttccaccgcg tctggctttg agtaccgttc ggattcagga aaagacggtt gaaattatcc
      181 cgaaagataa ccaggtttca ttacaattac cgccgctgtc ctattatgaa aatacactca
      241 cctttgaatt tgcggccatg tcatttattg acccggaagc cgtgaggtat cagtaccggc
      301 tggaagggtt tgaccatgac tggtcggcgc cggtcagcga ccggtttgtc cgctttatca
      361 atctgccgcc ggggaactac cggtttttca ttcgcgcgat ttcagcttcg ggggtctgga
      421 ccgacccaca aaacatccag gtcacgattc agcttccatt ctggcagacc tggtggtttc
      481 gatttctggt gcttctgctg gtaacgacga ccattggcgt tgtatacacc gccaatatct
      541 atcggatcaa acgaagacag gaagaaaaac tggcggtgct cagacagatt caggaacaac
      601 ggatcaagag cctgcgggaa ctgctggaaa gtattcgggt cattaactcc aatctggaac
      661 tggaaaacgt gctccaaaac attgccgaag aaagtgcccg gctggttgat ggcgaacccg
      721 gcggcattgg actggttgag aacggaaaag tctgttttcg gcgattgtgg cggcaggacc
      781 actgggaatc ctcgccactt gagtttcacc tgggtgatgg cattgccgga aaagttgccc
      841 aaaatgctcg accaatgatt gtgaatgagc cggttcaatc aaccgacgtg atttatccgg
      901 aactgatcga ggagtatttt gttcacggct ggatggatgt tcccattgtt gcccgctcag
      961 gtcaggtggt tggggtgctg gacgtcaggc gcaaatcagg gcggcctcca tttactgaaa
     1021 gcgactgcca cctgatcgaa gccctggccc atcaggcggc ggtggcgatt gaaaatgcag
     1081 ggctgtatgg cgagcttgag gaaaagaacc tcatggtggt cgaatccatg cacgaattag
     1141 aaaaactcta taagcaggag cgggaagtca cccgcgcctt gcaggatatt gaccggatga
     1201 agaccaattt catcaccgtg atgtcgcacg aaatgcgcac cccgctgacc gttattaaag
     1261 ggtacaacga ggcaattttg gaccagtatt ttggtccatt gactccaatc caggaaaagt
     1321 cattacaaac ctgtcagcga atggttgaac ggctggtgtg tagctttaac gatattttgg
     1381 aaatgctcaa aatcaacgaa ggacacgttg agctacgcta tgaacgggtc aatatccgga
     1441 cgctggttga cgaggtgtta agtgatctgg ccaactttat tgaaaaccga caccagcaca
     1501 ttacacttga ctcaccggct gagtttcaca tcgaaattga tagtgacaag attc
//
LOCUS       DAOQDO010001794         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90867_length_1554_cov_3.230821, whole genome shotgun sequence.
ACCESSION   DAOQDO010001794 DAOQDO010000000
VERSION     DAOQDO010001794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90867_length_1554_cov_3.230821"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..>1554)
                     /locus_tag="PKZ53_22725"
     CDS             complement(64..>1554)
                     /locus_tag="PKZ53_22725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43318.1"
                     /translation="RDKESKSGSFINGAMVTEKVLASGDEIILGDITAARIVFSCVAP
                     PVTSESVESRPSVSNILTAPTRAQKVLVPEMAGATIITDRQTRFLNTELMLQPQYVST
                     RTLQRMASLYEITHKILPVHDPMELVSMWLDCIFKALPVEHGVILIYNPASGQLETAI
                     NRDRGNTGALTTFSKTIVEKTFLENVAILASDASADERFSARESIIIQNIRSVLSAPI
                     SSKSRVWGVCYLYSRTQAALFDSEDLEFLMATAREVGLVMENLSLINELRATQEQLVK
                     SERMAAIGKLASSISHELRNRLALLSGAEILEIKYGNDPEVKQFTDMVLLGQERALAL
                     VEEIRAYAKNRPMDFEKIRRPIVPTIERTISLLRLDHGVKRRSLHFQFTESPELVFNE
                     EKVEQVLMNLVRNAVEATKEDTGQVIVRLVVENATVAIHVADNGCGIPAENLQRIWEP
                     FFTTKGDEGTGLGLDICRRIIEAHGGTITCQSQAGVGTCFTIRLPL"
BASE COUNT          370 a          415 c          378 g          391 t
ORIGIN      
        1 cctgtctctg tgttccgctg tgtccgctgt ggttcaaacg ttttggattt ttcgataacc
       61 gacttacaag ggaagccgaa ttgtaaagca agttcccact ccggcctggc tctggcaggt
      121 aattgtcccg ccgtgggctt cgataatccg acggcagata tcgagcccaa gacctgtccc
      181 ttcgtcaccc ttggtggtaa agaacggctc ccaaattcgt tgcaaatttt cggctggaat
      241 cccacaacca ttgtcagcca cgtgaatggc cacggttgca ttttccacga ccagccggac
      301 aatcacctga ccagtgtcct ctttggttgc ctcgacggcg ttgcgaacca ggttcatcag
      361 cacctgttcg actttttctt cattaaaaac aagttctggc gattcagtaa attgaaaatg
      421 gagcgaacgc cgtttgacgc catgatctaa tcggagcaat gaaatggtac gttcaatggt
      481 tggaacaatc ggacgacgaa ttttctcaaa atccatcggt cgatttttgg cataggcacg
      541 gatttcttca accagcgcca gagcgcgttc ctgtccgagc aacaccatat cggtaaattg
      601 tttgacctcg ggatcattgc catatttgat ttccaagatt tcggcaccac tcagcagcgc
      661 cagccggttg cgaagctcat gggaaataga cgaggcaagt tttccgatgg ctgccatgcg
      721 ttccgacttg accagttgct cctgagtcgc ccgtaattca ttgatcaaac tgaggttttc
      781 catcaccagg ccgacttcac gggccgtagc catcaaaaac tccaggtctt cagaatcaaa
      841 cagcgcggcc tgggttcggc tgtagaggta acaaactccc cagacccgac tcttggaact
      901 aattggcgct gaaagaactg accggatgtt ttgaatgatg atgctttcac gggctgaaaa
      961 ccgttcatcc gcactggcat cgctggccag aatggcgaca ttttccagaa acgttttttc
     1021 aacaatcgtt ttgctgaacg tggttaaggc cccggtattg ccccggtcac ggttgatggc
     1081 ggtttcaagc tgcccgctgg ccgggttata gatcaagatc acgccatgct cgaccggcag
     1141 cgctttaaaa atacagtcca gccacatcga aaccagctcc atcggatcat gaactggcag
     1201 aattttatgt gtgatttcat agagcgatgc catgcgctgt agcgtccggg ttgaaacata
     1261 ctgtggctgg agcatcaact cggtattgag aaaacgggtc tgccggtcgg taataatggt
     1321 ggccccagcc atttctggaa ccaacacttt ttgagcccga gtcggggcgg tcagaatatt
     1381 tgaaaccgac gggcggcttt cgaccgactc actggtaact ggcggagcaa cacaactaaa
     1441 cacaattcga gccgctgtga tatcccccaa aatgatttca tcaccgctgg ccagcacctt
     1501 ttcagtcacc atggcgccat tgataaagct ccctgatttg ctttctttgt cccg
//
LOCUS       DAOQDO010001795         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90978_length_1553_cov_3.432577, whole genome shotgun sequence.
ACCESSION   DAOQDO010001795 DAOQDO010000000
VERSION     DAOQDO010001795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90978_length_1553_cov_3.432577"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /gene="rbcL"
                     /locus_tag="PKZ53_22730"
     CDS             complement(<1..253)
                     /gene="rbcL"
                     /locus_tag="PKZ53_22730"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748132.1"
                     /note="type III RuBisCO; involved in carbon fixation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HNJ43319.1"
                     /translation="MTTGMVAKDRYKAGVLPYAKMGYWDGDYIPKDTDVLALFRVTPQ
                     EGVDAIEAAAAIAGESSTATWTVVWTDRLTACEVYRAKAY"
     gene            complement(316..1332)
                     /gene="fbp"
                     /locus_tag="PKZ53_22735"
     CDS             complement(316..1332)
                     /gene="fbp"
                     /locus_tag="PKZ53_22735"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNJ43320.1"
                     /translation="MSHDVINISRHILEQQRQSPGATGELSILLNTLALAAKISSHEV
                     NRAGLGNVLGHAGYQNVQGEQVTKLDIFTHNLLHGLLGRSGQVCVMASEEAEHPLEVP
                     EGYPNGKYVVLFDPLDGSANLEINASVGMIFSVFRRVSSGGKGTIEDCLQAGRHQVCA
                     GYVVYGASTMLVYSTGHGVHGFTLDPAIGEFLLSHENIRIPKKGTMYSINEGRSKGWP
                     PGIVEYLNSIKSGQNGPTYNLRYIGAAIADVHRTLLTGGIFLHPASSANPKGKLRLLY
                     EASPLAFLVEQAGGRASDGKQPILEIQPESLHQRVPMIIGSVDDVLTAERFIREAEAK
                     AYYA"
BASE COUNT          359 a          438 c          398 g          358 t
ORIGIN      
        1 gataggcttt ggcccgatag acctcacagg ccgttagccg gtctgtccag accaccgtcc
       61 aggtagcagt cgaagattca cccgcaatcg ccgccgctgc ctcgatggca tcaacccctt
      121 cctggggagt tacccgaaac aaagccagca catcggtatc tttggggatg taatccccat
      181 cccaatatcc catcttggca tagggcagca cccctgcttt gtaacggtcc tttgcaacca
      241 tacctgttgt cataaattcc tccgcaataa gcaaaacgtg agcgcttgag tcactgacgc
      301 tcaccaactt ttgccttacg cataatatgc tttggcttcg gcctcacgga tgaatcgttc
      361 agccgtgaga acatcgtcaa cgctgccaat aatcattggc acgcgctggt gtaaagattc
      421 cggctgaatc tccagaatcg gctgtttccc gtcagaggca cggcctccgg cctgctcaac
      481 caaaaatgcc agcggtgatg cctcatacaa caggcggagt ttccctttcg ggttcgctga
      541 actggctgga tggaggaaaa tccctccggt caagagagtc cgatggacat cggcgatggc
      601 tgccccgata tagcgcaggt tgtaggtcgg accattctgg ccgctcttaa tgctattgag
      661 atattccaca attcctggag gccacccttt ggaacgccct tcattgatgc tgtacatcgt
      721 cccttttttt ggaatccgga tattttcatg cgacagcaga aattccccaa tcgccggatc
      781 aagggtaaac ccgtgaaccc catggccggt cgaatacacc agcatcgtac tggcgccata
      841 gaccacatac ccggcgcaaa cctgatgtcg cccggcctgc aggcaatctt caatcgttcc
      901 cttcccgccg gatgaaacac gtcgaaacac tgaaaaaatc attccgacgc tggcattgat
      961 ttccaaattg gccgaaccat ccagcgggtc aaacaaaaca acatattttc cgttggggta
     1021 gccctcggga acttcaagtg ggtgttcggc ttcttcagag gccatcacac acacctgacc
     1081 tgagcgtccc agcaggccat gcagcaggtt atgggtaaaa atatcgagtt tggtcacctg
     1141 ctcgccctgc acattttgat atccggcgtg gccaaggaca ttgcctaacc cggctcgatt
     1201 gacttcgtgg gaactgatct tggcggccag ggccagggta ttgagcaaaa ttgatagctc
     1261 gccagttgcc ccaggcgact ggcgctgttg ttcgagaatg tgtcttgaga tgttaatgac
     1321 gtcatgtgac ataagttgaa cctttttggg gctgagaaat ccagggctca gggcttgggg
     1381 ccgagaaatc cagggctggg ggcttggggc tgagaaatcc agggctgggg gcttggggct
     1441 gaagattcgc aagctcaggg ctgaagaaaa cgggtttaat accaagcccg aagtcttcag
     1501 cccgacttct tcagcccgaa gtcttcagcc cgacttcttc agcccgaagt ctt
//
LOCUS       DAOQDO010001796         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90988_length_1553_cov_2.979973, whole genome shotgun sequence.
ACCESSION   DAOQDO010001796 DAOQDO010000000
VERSION     DAOQDO010001796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90988_length_1553_cov_2.979973"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..>1553
                     /locus_tag="PKZ53_22740"
     CDS             229..>1553
                     /locus_tag="PKZ53_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HNJ43321.1"
                     /translation="MTNIIEVLDTDPISARAEADSENRDFSLGIQGFTYGDLYRPERL
                     KELADTFYAAMAEDDPELAATFEGYRLSGGKEISPKAESDLLVRTAPHLSRFVARMFG
                     IEAELETLGYVITRESDVFVMKREFIQRRVVKRPASEYSVERRAHLEQSAQTLLEIGF
                     DLSDDLELAVAQAVASLLRWDKAFEKFLAKKAELSEDIRQCVTNLYQVLSENEASRTA
                     WAEILPGAASDLTDEARQTFLKQVLDRLEGWLHALSTTPETRHRIKDWVSFRLPEAVE
                     YQHLVQINRPRPELPEMMDGPHEHFRHRDGFHLTDPRYTRREVSSETDYCIICHPREK
                     DSCSKGFFEKDGSYRKNPLGIPLTGCPLDEKISEAHAMQRQGDSLAALAIILVDNPMA
                     PGTGHRICNDCMKACIFQKQSPVNIPQAETGILTDVLGLPFGFEIYALLT"
BASE COUNT          375 a          422 c          392 g          364 t
ORIGIN      
        1 tcttggttct tggttcttgg ttcttggttc ttggttcttg gttctacttc gaaagtattg
       61 attcctaagc accaagcact aagcactaag caccaagcac caggcaccag tcaccagtca
      121 ccagccccgg tacggggatg ggcacattga ttgcatcacc agttaagttc ctgcatcatt
      181 tttcggaaaa atccgcactt tttggaatgt cctggttgac gtttacttat gacaaacata
      241 atagaagttt tagatacgga tccgatttcg gctcgtgccg aagcagatag tgaaaatcgc
      301 gatttttcac ttggaattca agggtttacc tacggtgacc tgtatcgtcc ggaacggctc
      361 aaagagttag ccgacacgtt ttatgccgcc atggccgaag acgatccgga gctggctgcc
      421 acgtttgagg gttatcgcct gagcgggggt aaggaaattt ccccaaaagc tgaatcagac
      481 ttgctggtac ggaccgcacc tcacctgagc cggtttgtcg cccgaatgtt tggcatcgaa
      541 gccgagcttg aaacacttgg ctatgtgatc actcgcgaaa gcgacgtctt tgtgatgaaa
      601 cgcgagttca ttcagcgccg ggtcgtcaaa cgtccagcat ctgaatacag cgtcgagcgg
      661 cgtgctcacc ttgagcagtc ggcccagacg ctcctggaaa tcggatttga cctgagcgac
      721 gaccttgaac tggccgttgc ccaggcggtg gcttccctgc tgcgatggga caaagcgttt
      781 gaaaaattcc tggccaaaaa agctgaactc tctgaagaca tccggcagtg tgtaaccaat
      841 ctgtatcagg tgctgtctga aaatgaagcc tcccgcaccg cctgggccga gatactgcct
      901 ggtgctgcaa gtgatctgac tgacgaagcc cgccaaacct ttctcaagca agtgcttgac
      961 cggttggaag gctggctgca cgcactatca accacaccgg aaacccggca tcggatcaag
     1021 gattgggttt ccttccgcct tcccgaggca gttgagtatc agcatctggt gcaaatcaac
     1081 cgtccacgtc cggaactccc ggaaatgatg gatggcccac acgaacattt tcggcatcgc
     1141 gatggctttc acctgactga cccacgctat acccgccgcg aagtctcaag cgaaactgat
     1201 tactgcatca tctgccaccc acgtgaaaag gattcctgtt caaaaggctt tttcgaaaaa
     1261 gacggttcct accgaaaaaa tccgctcgga atcccgctga ctggctgtcc actggatgaa
     1321 aaaatctctg aagctcatgc catgcaacgc cagggcgatt cactggccgc gctggcgatc
     1381 attctggttg acaatccaat ggctccaggc accgggcacc gcatctgcaa cgactgtatg
     1441 aaagcctgca ttttccaaaa acagtcgcca gtcaacatac cgcaggccga aacgggaatt
     1501 ctgaccgatg tgctcgggct gccgtttgga tttgaaatct atgcgttgct gac
//
LOCUS       DAOQDO010001797         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_90993_length_1553_cov_2.814419, whole genome shotgun sequence.
ACCESSION   DAOQDO010001797 DAOQDO010000000
VERSION     DAOQDO010001797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90993_length_1553_cov_2.814419"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..>1553
                     /locus_tag="PKZ53_22745"
     CDS             258..>1553
                     /locus_tag="PKZ53_22745"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNJ43322.1"
                     /translation="MSRPSKKNLSSPDIPVRAERSLLELPLFMVDREARFELRVIEQE
                     KQTSEGPLTSRIEIGFNQFGTLIPRDRLVYLALTAIWQEQGKPVNFVVSLRRIAKLLN
                     MGWGKKQILALKESLHRLKGVNLIFINSLFDASKDKTVEKETSFTFLDKLEIFSISEN
                     GKTNKADCVVAFNHVIDSNLRLGHVRPVMLPTLTNLRSDLAQSLYLLLDAQTCNPNKK
                     YSISSKKLLAELGLTSSRYSFLSRRKEILEKAIHELDGIAVLGGTLKVSLEPTRDGTD
                     LKLTAERIAVASPDSEMEMAAVLIEPPSPRLKDLMDPTIFIGLIFDRFGIKRKPKVKE
                     LEFARNVVSQYGIDLDVARHIVEFAFRDASRTKFLLRDARGLIQYIEPALESYEEFAD
                     SQGNDLEEASFAETYHQITEFEETELFEKAGEKVLSSMDT"
BASE COUNT          430 a          326 c          362 g          435 t
ORIGIN      
        1 gctggtgtac ttctcgacaa tcgctggtgt acttctcgac aatcgctggt gtacttctcg
       61 gcaattgctg gtgtacttct cgacaaaaat tactttttaa tggtacttaa cctctttatt
      121 ttcatcggct tccaggaagt caaattttac tagagtattt agagtatatt tagagtattt
      181 aagagtatat taaacacaca ctgtgtggta ttttcacttt gtctggattt cctagaaagt
      241 gactgttgta aatccgaatg agccgtccat caaagaaaaa tctttcgtca cctgacattc
      301 ctgtacgagc tgaaagaagc ctgctggaat tgcccttatt tatggtggac cgagaggcaa
      361 ggtttgagct acgggtgatt gaacaagaaa aacaaacttc tgaagggccg cttacttccc
      421 gaatagaaat tgggtttaat cagtttggga cgcttattcc gagagataga ttggtttatt
      481 tggcattgac cgccatttgg caagaacaag gaaagccagt caactttgtt gtttcgttgc
      541 gcagaattgc caaactcttg aatatgggct gggggaaaaa acaaatcctg gcactcaagg
      601 aatccctcca ccgtctgaaa ggggtgaatc ttatttttat caacagcctc tttgacgcca
      661 gcaaagacaa aacggtggaa aaggaaacga gctttacctt tttggataaa cttgaaattt
      721 tttccatttc cgagaatgga aaaaccaaca aagccgactg tgtcgtggcc tttaatcacg
      781 tgattgattc aaacttgcgt cttggtcatg ttcgcccagt gatgcttcca acactaacga
      841 acctgcgttc cgatctggct caatcgttgt acctgctttt ggatgctcaa acttgtaacc
      901 caaataagaa atactcgatt tccagcaaaa aattgctggc agagctgggg ctgacgtcct
      961 ctcgatatag ctttttatca cggcgaaagg aaattcttga aaaggcgatc catgagcttg
     1021 atggaattgc cgtgctgggc ggaaccttaa aggtgagttt agaaccaacc cgcgatggaa
     1081 ccgaccttaa attgaccgcc gaacggattg ccgtcgcctc gcccgattca gaaatggaaa
     1141 tggcggcagt gttaattgag ccaccatcgc cgcgactcaa ggacctgatg gatccaacta
     1201 tttttatcgg gttgattttt gaccggttcg gaatcaagag aaaaccaaag gtgaaagaac
     1261 tggagtttgc ccggaatgta gtcagtcagt atggcataga tcttgacgtg gcccgccata
     1321 tcgttgaatt tgccttccgg gatgccagtc gaaccaaatt tctgttacgg gatgcacgtg
     1381 gccttatcca gtacattgag cctgctcttg aatcatatga agagtttgcc gattctcaag
     1441 ggaatgattt ggaggaagcc tcattcgcgg agacctatca tcaaatcact gaatttgagg
     1501 aaacagagct tttcgaaaag gcgggtgaaa aggttctttc cagtatggat acc
//
LOCUS       DAOQDO010001798         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91088_length_1552_cov_3.420842, whole genome shotgun sequence.
ACCESSION   DAOQDO010001798 DAOQDO010000000
VERSION     DAOQDO010001798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91088_length_1552_cov_3.420842"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKZ53_22750"
     CDS             complement(<1..273)
                     /locus_tag="PKZ53_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43323.1"
                     /translation="MARLDVASKALHYQVLPEEGQEEGGGKPVTGATNNTVWSFRRPV
                     VIVELRQVGYGLLETWKPRNPTLAAATIVPAAEMPCRHAKVLTCGDA"
     gene            328..744
                     /locus_tag="PKZ53_22755"
     CDS             328..744
                     /locus_tag="PKZ53_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HNJ43324.1"
                     /translation="MIPEKSDTSSPKFECRPWGSYTVLDEADGYKVKRIEVLPGKRLS
                     YQKHTHRTEHWFVVQGIGKVTLNGDEVLLEPGQVIDIPVLAPHRIENPGLELLVFIEV
                     QRGDYLGEDDIERLQDDFGRLPGPWLDQVADLAGVS"
     gene            complement(960..>1552)
                     /locus_tag="PKZ53_22760"
     CDS             complement(960..>1552)
                     /locus_tag="PKZ53_22760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ43325.1"
                     /translation="QSVDILRVAIEPHFFDRIATQEGMATTSELPLRRDIVDARIFNL
                     AQVLKVELEDQCPNGAVFGDSIGTALVVHLLKRYATRPLVIPNYASGLEPAQLRRVTD
                     FLRANLDQKVTLDQLAELVDVSQFHFARLFKRSTGLSPHQYLIKLRVERAKDLLLNRN
                     LSIADVAQAVGFTDQSHLTNHFKSLLGISPGKYRSR"
BASE COUNT          398 a          397 c          394 g          363 t
ORIGIN      
        1 tgcatcgccg caagtcagga ctttcgcatg ccggcaaggc atttctgcgg ctggcacaat
       61 cgttgcagcc gccagggtag gattccgggg tttccaggtt tcaagcaggc cataaccgac
      121 ctgccgcaat tcaactatca ccacaggacg acgaaaactc caaactgtgt tattcgttgc
      181 cccagtgacc ggtttcccac ccccctcctc ttgaccttct tcgggtaaga cctgataatg
      241 cagcgctttg gaagcaacat ccaaacgggc caccgcgtca cactccggcc caacacgaaa
      301 agcaccactg agtttgagga ataatctgtg atacctgaga aatccgatac ttcttccccg
      361 aagtttgaat gtcggccctg gggaagctac acggttttgg acgaagccga tggctataaa
      421 gtcaaacgca tcgaagtttt acctggaaag cgactgagtt atcagaaaca cacacaccgc
      481 actgaacact ggtttgtggt ccaaggaatt ggaaaagtca cgctcaatgg agatgaagtg
      541 ctgctcgaac cagggcaggt aattgacatt ccggttttgg cgccgcaccg cattgagaac
      601 cccggcctgg aattgcttgt gtttattgaa gttcaacgcg gtgattacct gggcgaagat
      661 gatatcgaac gccttcaaga cgattttggc cgccttcctg gaccctggct ggatcaggtt
      721 gctgatctgg caggtgtttc ataatccagt ttcatcagac ccagacaaaa atcagaccag
      781 tgattgggat gaacaccatc ctggtcactg gtctgtgagg ttctgggtaa agaataaaaa
      841 tccgttctga cgaataggat tttcggggtt cagctcagag aacctgaccc gatgtcaaca
      901 gtagccggag acacaggccc gtgtcttgga ggtttgtgcg gaaaaagtgg agcttcttgc
      961 tatcgggaac gatattttcc aggcgagatg ccaagcaggc ttttaaaatg gttggtgaga
     1021 tggctctgat cagtaaaccc aacggcctgg gcgacatcgg caatactcag attccgattc
     1081 agcaataaat ctttcgcccg ttcgacccga agtttgatca aatattgatg cggagaaagt
     1141 cctgttgacc gcttaaacag tcgggcgaaa tgaaactgac tgacatcaac cagttctgcc
     1201 agttggtcga gtgtcacttt ttgatccaga ttggctcgca ggaaatctgt cacacgacgc
     1261 agttgggctg gttcgagtcc actggcataa ttaggaatca ccagtggccg ggtggcatac
     1321 cttttgagca aatgaaccac cagtgccgtg ccaatcgaat caccaaacac ggcaccgttg
     1381 gggcactggt cttcgagttc aactttgagc acctgggcca gattaaaaat cctcgcgtcc
     1441 acaatatcgc gccgcaacgg gagttcgctt gtggttgcca ttccttcctg ggttgcaatc
     1501 cgatcaaaaa aatgcggctc aatcgcaacc cgcagaatat caaccgattg at
//
LOCUS       DAOQDO010001799         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91104_length_1552_cov_2.924516, whole genome shotgun sequence.
ACCESSION   DAOQDO010001799 DAOQDO010000000
VERSION     DAOQDO010001799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91104_length_1552_cov_2.924516"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            609..>1552
                     /locus_tag="PKZ53_22765"
     CDS             609..>1552
                     /locus_tag="PKZ53_22765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ43326.1"
                     /translation="MGNQTESSDKQFSPLAWDGQNIHLDDIGAALAVCTTDGELVGAT
                     ASARVILTHFSNPIPGYPQLLPVGLWQDLMASPLGEIVQWRPSDWAQDTYLGCTRYRL
                     GESHAVLVMRVLRDRSTILSKRLHEQRLEATGRLVANIAHELRTPLSSILFNADFMVL
                     RWDQLPPDLALESLKEIQTACRRMQRTIEGLLDFARLGVQRTSSVSLRENFERTTSLL
                     RPTFRDGGHTIQVSIRPDAEWVRGNPLVLEQVFVNLMMNAIESAPRGVRIEIESSREV
                     YQVLDPSQEFVCVRVRDTGLGVATEIRNRIFEPFFTTKP"
BASE COUNT          371 a          344 c          417 g          420 t
ORIGIN      
        1 tagcgtcgtg ggtacacgac tcaagcttgg cccttgaggg ccgagtctct tgactgatgt
       61 ctaactgggc aattcaagat ggggagaaga tcttactgta cttttatcgg ttcacttcgt
      121 caattattgc ctctggtgag agagcttcat cgaaactgct tgccattcct gttttgagat
      181 ttggtgtctt gtttttggac accaatgatt gtttaagcga agtgactggt agatggttca
      241 gtgaaatcga gggcaatgta taaagaaaat caaggagttg accagtgccg gatcaaccag
      301 ggtggaggta tctctggtgt ggcacgacaa atgcacaggt ggtgtgtctg cgttcaactc
      361 aatattttct gccggcgttt ccagggggaa cccatttctg aaaaactaca actgacctcc
      421 ctggcagtgc aaacccaaaa acctgaacag aagaggatat ccgtgttccc aactgaaaac
      481 gggaagttga ttttttacag ttccttaggg tttgttgagc aaaaatcgca gttccaatcc
      541 gtgatgtgat atctaacggt cagatactaa atttgcaaag aggtacggag aaagggatag
      601 gatttgttgt gggcaaccaa actgaaagtt cagacaaaca attttcccca ctcgcctggg
      661 atggacaaaa tattcacctg gacgatattg gggctgcact ggctgtctgt accactgatg
      721 gtgaactggt tggtgcgacg gcttcggcca gggtaattct gacgcatttc tcaaatccaa
      781 ttccagggta tccgcaactc cttccagttg ggttatggca ggatttgatg gcttctccgc
      841 tcggagaaat tgtccagtgg cgaccctctg attgggcgca ggatacctat ttgggttgta
      901 cccggtatcg tctcggggag agtcacgccg tgctggttat gagggtgctc cgagatcgga
      961 gcaccatttt gtcaaaacgg cttcacgagc aacgacttga agccactggc cgactggtgg
     1021 ccaatattgc gcatgagcta cgaactcccc tgtcgagcat tctgtttaac gctgatttta
     1081 tggtgcttcg atgggatcaa ctgccaccgg atctggcgct tgaaagtttg aaagaaattc
     1141 aaacagcctg tcgtcgaatg caacggacaa ttgaaggctt gcttgatttt gctcgacttg
     1201 gtgtccaacg aacctcttcg gtttcactgc gggaaaattt tgagcgaacc accagtttac
     1261 tgcggcctac gttccgtgat ggcggccaca ccatccaggt ttcaattcgc cctgacgctg
     1321 agtgggtgcg cggaaacccg ctggtcctgg aacaggtttt tgtgaattta atgatgaatg
     1381 cgattgaatc agctccaagg ggggttcgga ttgaaatcga gagttcccgt gaagtctatc
     1441 aagtccttga tccaagccag gagtttgtgt gtgtccgggt gcgggatact ggcttagggg
     1501 tggcgactga aatccgcaat cgcatttttg agcctttttt taccaccaaa cc
//
LOCUS       DAOQDO010001800         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91241_length_1551_cov_2.440508, whole genome shotgun sequence.
ACCESSION   DAOQDO010001800 DAOQDO010000000
VERSION     DAOQDO010001800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91241_length_1551_cov_2.440508"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..>1551
                     /locus_tag="PKZ53_22770"
     CDS             286..>1551
                     /locus_tag="PKZ53_22770"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43327.1"
                     /translation="MQFPALFRTGLAIFLLLVQSAGIVTVAHPAIKISQTDPPPQEKP
                     KITAVLSAGSKLERAVTGEESNTFELSLSQGQYLELEVEQKELKLSLSLMDPAGKSVL
                     EIDSAYDVGSTEFVGFVAETTGTFLVTVWTEGTPRNRPRKYEIRVVDIRPAQPADLER
                     AAAIRSFVEAQELADGKEPDAKSKALERLYPLLARFRAIQDKHWEVTTLIEISRWLNE
                     TGQKRKAFDRCQEVLPLLLNLNEKHLATSILIDIATLHNTFGQPQKAIEALQQAISMA
                     QSVNHKLGLSVAYTNLGVAYRNLGENYKAVEYQKRALDLRREMDDMENSGALLLNMGV
                     AYGTIGETQLALDHFEDALKLLRKRGDQKGQAFTLISMGREYINLGDPATAIQLYQQG
                     LELTRELNDIRAEANILLALATTQQENDPH"
BASE COUNT          404 a          398 c          386 g          363 t
ORIGIN      
        1 aacagtactg ctgaaaagga tggatgacaa tgacaacgtt tgatctatcc ctttttagat
       61 aaatactttt tggagtgcgc tgacttgtca gcgctttgga tctcggcgac ttgtcgctgg
      121 aaatgaaagc tgtgacaagt cacagcactc caaatatttt tcctggtggg caaagcggag
      181 agtctttatt tcccgttcgc aaatagtcag ttttagagta agctcgactc agtcacagcc
      241 cctgtgtgct ttcagattct caggtttccc tctctggagt tccctatgca gtttcccgca
      301 ctgtttcgca ctggtctggc gatttttctt ctgctggttc aatcggctgg gattgtaacg
      361 gtggcgcatc ccgccatcaa aatttcccaa acagatccac caccacaaga aaaacccaaa
      421 atcacggcgg tgttgtcggc tggatccaaa cttgaacgag ccgtcacggg tgaagaatcc
      481 aataccttcg aactctccct cagccagggt caatacctgg aactcgaagt cgagcaaaaa
      541 gaactcaagt tgagcctttc gctgatggat cctgccggga aatcagtgct ggaaattgat
      601 agcgcctatg atgtcggctc aaccgagttc gtcggatttg tggctgaaac aacaggcacc
      661 tttctggtca cagtctggac ggagggcaca ccccgcaatc ggccccgcaa atatgaaatc
      721 cgggtggttg acatccgccc tgcccagcca gccgatcttg aacgggcggc ggcgattcga
      781 agctttgtcg aggcccagga gctggctgat gggaaggagc ccgatgcgaa atccaaggca
      841 ctggagcggc tctacccatt actggcgcga tttcgggcca ttcaagacaa acattgggaa
      901 gtcaccacat tgattgaaat ctcacgctgg ctcaacgaaa ctggccagaa acgcaaagcc
      961 tttgaccggt gtcaggaagt attaccgttg ctgctgaact tgaatgaaaa acaccttgcc
     1021 acttcaatcc tgattgatat tgccacacta cacaacacgt ttggtcaacc tcaaaaagcg
     1081 attgaagccc tgcagcaggc aatttccatg gctcaatccg tcaatcacaa actgggtctt
     1141 tccgtggcct ataccaatct gggtgtggcc tatcgaaatc tcggtgagaa ttacaaagca
     1201 gttgagtatc aaaagcgggc gctggacctg cggcgtgaaa tggatgatat ggagaattca
     1261 ggcgccctgt tgctcaatat gggcgtcgct tatggaacaa tcggagagac tcaactagcc
     1321 ctggatcatt ttgaggatgc cctcaagctt ttacgcaaac gaggtgacca aaaaggtcag
     1381 gcgtttaccc tgatcagtat gggccgtgag tatatcaatc tgggtgatcc agcaactgcc
     1441 atccaactct atcagcaggg tctggaattg acccgagaat taaacgatat ccgggcagaa
     1501 gcgaatattc tcctggcact ggccacgacg cagcaagaga acgacccaca c
//
LOCUS       DAOQDO010001801         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91330_length_1550_cov_2.831438, whole genome shotgun sequence.
ACCESSION   DAOQDO010001801 DAOQDO010000000
VERSION     DAOQDO010001801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91330_length_1550_cov_2.831438"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="tilS"
                     /locus_tag="PKZ53_22775"
     CDS             <1..1094
                     /gene="tilS"
                     /locus_tag="PKZ53_22775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNJ43328.1"
                     /translation="LHSSFSILHSQFFILNSSMVCDPLWPPIARYLKQTSALQDVWCL
                     VVAVSGGPDSVALLHILKAEGLRTYPGLHLHVAHLNHGLRGSAAIADAQFVYNLATQL
                     ELPVTIETEDVSAQAQHRKQNWEATARLVRYGFLHRVARNTGAQAIATGHTKTDQAET
                     VLMRLIRGTGLDGLGSIQPVQVLESDTPFASDPVRLIRPLLAVERFEIEAYLERQQIK
                     ACHDETNDLIELTRNQIRHQVMPLLTRLNSKAVDHLAGLAALAHQEADFFKPLVDQWI
                     AQFSVVMTGQVQVPLAELRQLHPVLVGRVIHQLVQQPEFLSANQKQIQQICDVLVWGK
                     TGGKAIDLPGNRRVRRIRNYLLFERSNNI"
BASE COUNT          360 a          377 c          400 g          413 t
ORIGIN      
        1 ttcttcattc ttcattctca attcttcatt ctcaattctt cattctcaat tcttctatgg
       61 tttgtgatcc gttgtggcct ccaattgcca gatatctcaa gcagacttca gcgttgcagg
      121 acgtttggtg tctggtggtt gcggtatctg gcggcccgga ttcagtggcg ttgctccata
      181 tactcaaggc tgaaggtctt cgcacgtatc caggattgca cctccatgtt gcccacctca
      241 atcacggttt gcggggaagt gccgccatag ctgatgctca atttgtctat aaccttgcga
      301 ctcagcttga gttaccagta acgattgaga ctgaggatgt atccgctcag gcacaacaca
      361 gaaaacagaa ctgggaggcg acggcgcgtc tggtccgcta tggttttctg caccgggttg
      421 cccgcaacac aggtgcacag gccattgcca ctggtcacac caaaaccgat caggccgaaa
      481 ccgttttgat gcgattgatt cggggaactg gacttgatgg attggggtct attcaaccag
      541 ttcaggtgct tgaaagcgac acgccttttg cttctgaccc tgttcggctc attcgtccgt
      601 tgttagccgt tgaacgcttt gaaattgaag cctatctgga acgacaacag ataaaggcct
      661 gtcatgatga aaccaacgac ctgatcgaat tgacccgaaa tcagattcgg catcaggtta
      721 tgccgctttt gacccggtta aactcgaaag cggttgatca tctggctggt ctggccgcgt
      781 tagctcatca ggaagccgat ttttttaagc cgctggttga ccagtggata gcccaatttt
      841 cagttgtgat gactgggcag gttcaggtgc ccttggctga actcaggcag ttgcatccgg
      901 ttctggttgg tcgggtgatt caccaactgg tccagcaacc tgaattccta tcggccaatc
      961 aaaagcagat tcagcagatt tgtgatgtgt tggtctgggg aaagacgggt ggcaaggcga
     1021 ttgacctgcc ggggaaccgg cgtgttcgtc gcatccggaa ctatctcttg ttcgagcgtt
     1081 caaataacat atagaaaata ttgggttttt gagactaaat tctggcaagc ctcaggtaaa
     1141 gagggctagg caggatgatt taccataaaa aaactgagaa agccccgttc tcaactgcga
     1201 agccgtaggc cgggggatga atcagcttga tctttttgat ttcctaaatt agccatgtta
     1261 ccgttatctc actccatgag ccatatataa ggcatattga tccgtatagc gtcatcgctc
     1321 ggacatggag cgttggaaat ttcggtttcg gtgattctcc ggaatcacct ttggatcgac
     1381 attcgcgtca tcgaaccttg acgccgcacg gtcgatccca aacgtgtgga gcgaacaaaa
     1441 tgcgaccgag ggaaatttct tcccgaaacg gtactccaca taccgttcga gcgtttcgtg
     1501 cgaaagctgg aaagtgccac ttagagccgt cggctcccag gcaggatcgg
//
LOCUS       DAOQDO010001802         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91439_length_1549_cov_2.511379, whole genome shotgun sequence.
ACCESSION   DAOQDO010001802 DAOQDO010000000
VERSION     DAOQDO010001802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91439_length_1549_cov_2.511379"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKZ53_22780"
     CDS             complement(<1..729)
                     /locus_tag="PKZ53_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNJ43329.1"
                     /translation="MTTSVTTSTVTTSLTREQKLELYHYLRQTRLLEERLTNLYRQTK
                     VIGGLFRSLGQEANAVGTAYALDKSKGDMITPLIRDLGAVLVMGGTPREILLQYMARR
                     DSPSRGRDLNIHFSDLNRGFIGPISHLGDLMPVMTGVALASRMKKQDRVCMVYMGDGA
                     TSTGAFHEGLNFAAVQKLPIVVVVEHNGYAYSTPTSKQMAVDNIVEKAVGYGIRGEMV
                     DGNDVLAVYETTRDAVEYARSGQGT"
     gene            complement(884..>1549)
                     /locus_tag="PKZ53_22785"
     CDS             complement(884..>1549)
                     /locus_tag="PKZ53_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43330.1"
                     /translation="GVDLTLRNQAGERLSLSVDKVCVTVGRVPQSAGLGLEWTQASIK
                     KGYVQVNKMLQTAEPTIYAVGDVADTVWLPHVAASEGLLAVGHILERDEVDPINYAHV
                     PSCTYSDPEVASVGLTEAAAKARGHKVRTGSFPFVANTQARVLNQTEGMVKLVSDAQY
                     DELLGVHIVGARATELIAEACIALRGEMTIDELIRTLHAHPTLSESLWEAALTIHSRP
                     IRF"
BASE COUNT          345 a          440 c          414 g          350 t
ORIGIN      
        1 agttccctga ccagagcggg catattcgac cgcatcgcgg gtggtttcat acaccgcaag
       61 cacgtcgttt ccatccacca tttcaccacg gataccatac ccgacggcct tttcaacaat
      121 gttgtcaacc gccatttgct ttgacgtcgg cgttgagtag gcatacccgt tgtgctcaac
      181 caccactaca atcggtaatt tttgaacggc agcaaagttg agtccttcgt ggaaagcgcc
      241 ggtcgaagtc gcgccatcgc ccatatacac catgcacacg cggtcctgtt ttttcatccg
      301 cgacgccagc gctacaccgg tcatcaccgg catcaggtcg ccaaggtgcg aaatagggcc
      361 gatgaaccca cggttcagat cgctgaagtg aatgttaagg tcacgtccac ggcttggact
      421 atcgcggcgg gccatatatt gaagcaaaat ttcgcgtggc gtcccaccca tcaccaggac
      481 cgcccccagg tcgcgaatca acggcgtgat catatcgcct ttggatttat ccagcgcata
      541 ggccgtccca accgcattgg cttcctggcc gaggctgcga aacagaccgc cgataacctt
      601 ggtctggcga tagagattgg tcagacgttc ttcgagcagg cgggtttgcc gcagatagtg
      661 atagagttca agcttctgct cacgcgtgag cgaagtggtt acagtgctgg tggttacaga
      721 agtagtcata aaagtccgta attgattttg aaaaatgaaa aaaaggtggg cggcaacccc
      781 ctggattgca cgccaaattc ggaatgcact ctttagccgt ttttttgaaa aacagcaatc
      841 gaaaaattga cgctgactcc caacccataa ccacggcgat tgattagaat cgaattgggc
      901 ggctgtggat cgtcaatgcc gcttcccaca aagattcgga gagcgttgga tgggcgtgga
      961 gcgtgcggat caattcgtca atcgtcattt ctccacgcag ggcaatgcac gcttcggcaa
     1021 tcagttcggt ggctcgtgcg ccgacgatat gaacgccgag gagttcgtca tattgcgcat
     1081 cgctcaccag tttgaccatg ccttcggtct ggttgagcac ccgtgcctgg gtgttggcca
     1141 cgaatgggaa ggatccggtt cggactttgt gtccacgggc tttagcggcg gcttcggtca
     1201 gcccgacgct ggccacttct gggtcgctgt aggtgcaact cggcacgtga gcataattga
     1261 ttgggtcaac ttcgtcgcgt tcaagaatgt gccccaccgc cagcaagcct tccgaagccg
     1321 cgacatgcgg cagccagacg gtgtcggcca catcacccac ggcataaatc gtcggttctg
     1381 ccgtctgaag cattttgttg acctgaacgt agcccttttt gatagatgcc tgggtccatt
     1441 ccaatcccag cccggcactc tggggaactc gcccgacggt cacacacacc ttatcaaccg
     1501 acagagaaag ccgttctccg gcctgatttc ggagcgtcag gtcaacgcc
//
LOCUS       DAOQDO010001803         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91539_length_1548_cov_2.700603, whole genome shotgun sequence.
ACCESSION   DAOQDO010001803 DAOQDO010000000
VERSION     DAOQDO010001803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91539_length_1548_cov_2.700603"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1548
                     /locus_tag="PKZ53_22790"
     CDS             128..>1548
                     /locus_tag="PKZ53_22790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43331.1"
                     /translation="MSNNYSATQWERMKELFNEVVDCPPQAREALLNARCGENELLRK
                     AVWSLIESFEKAGSFIDIPPAMNACSGLMENEEILVPEQRIGPYQIVRELGSGGMGAV
                     YLAKRVDEQFHKLVAIKIVKPTFWNTVVFQQFRKERHILAGLEHPNIARVLDGGTTSE
                     GLPFLVMEYIEGTSIDTFCNQHKLSIGERLPLFLKVCAAVQSAHQKLIVHRDLKPGNI
                     LVTAEGEPKLLDFGIAKLLDPNAWDASLEQTQTGLRMMTPRYASPEQIRGESVTTATD
                     VYSLGIVLFELLTGIHPYLHAQSNRIEIEQAICDTDPHRPSTAINRLNTRERQDSTTD
                     SINPQEIVAQRSESSPKRLRQRLEGDLDTILLKALQKDPAQRYSSVEQFAADIRRHLD
                     GLPVLAQPDSSWYRTRKFIKRQRGLVVAAGLIVLTLTGGVSATLYQAYVAKLEKERAI
                     RRFQEVRELASSFLFEFHDSIRD"
BASE COUNT          398 a          390 c          395 g          365 t
ORIGIN      
        1 aaccaggaac caggaaccag gaaccaggaa ccaggaacca ggaaccagga accaaaaacc
       61 aggaaccaaa aaccaggaac caaccagttt cttcattctc aattcttcat tcttcatttg
      121 gttttttatg tccaacaact attccgcaac ccagtgggaa cgaatgaaag agctctttaa
      181 tgaggtggtg gattgccctc cgcaggccag agaagctctt ctcaatgccc ggtgtggaga
      241 aaatgagttg ttgcgcaagg cggtctggtc actgatcgaa tcgtttgaga aagccgggag
      301 ctttattgat attcctcctg ccatgaacgc ttgttccggc ttgatggaaa atgaagaaat
      361 tctcgtccca gaacaacgga ttgggccata ccagattgtt cgggaactag gaagcggtgg
      421 aatgggggcg gtttatctgg cgaaacgggt tgatgaacag tttcacaagc tggtcgccat
      481 caaaattgtc aaacccactt tctggaacac cgttgttttt caacagtttc gcaaagaacg
      541 tcacatcctg gctggcctgg aacacccaaa cattgcccgc gtgctcgatg gtgggaccac
      601 cagcgagggc cttccatttc tggtgatgga gtacatcgaa ggaacgtcca ttgatacctt
      661 ttgtaatcag cacaaactga gtattggtga acggttgccg ttgtttctca aagtttgtgc
      721 tgccgttcaa tcagcccatc agaaactcat tgttcaccgg gatttgaaac ccggcaatat
      781 tctggtgacg gcggaaggtg agccgaagct cctggatttt ggcattgcga aattgcttga
      841 tccaaacgcc tgggatgctt cgctggagca aactcaaacc gggttgcgga tgatgactcc
      901 acgctatgcc agcccggaac aaatccgagg ggaatcggtc accaccgcta cagatgtgta
      961 ttcgctcggg attgtgttgt ttgagttgct gaccgggatt catccttatc tgcacgccca
     1021 gtcaaaccga atcgaaattg agcaggcgat ttgtgacact gacccacatc gaccaagcac
     1081 ggcgatcaat cggttgaata ctcgcgagcg ccaggattcg accacagatt ccatcaatcc
     1141 acaggaaatc gttgctcagc ggagcgaatc aagtccaaaa cgcctgcgac agcgactgga
     1201 aggtgatctg gacacgattt tgctgaaagc cttacaaaaa gatccagccc aacggtattc
     1261 gtcggttgaa cagtttgccg ctgacattcg ccgccatctg gatggattgc ccgtcctggc
     1321 tcaacctgat tcaagctggt atcgcacccg gaaattcatc aagcgtcaac ggggtctggt
     1381 cgttgccgcc ggactgattg tcctcacgct gaccggaggt gtcagtgcca cgctctatca
     1441 ggcatacgtg gccaaacttg aaaaggaacg cgctatccgt cgttttcagg aagtgagaga
     1501 actggccagc tcatttctat ttgaatttca cgactcgatc cgggattt
//
LOCUS       DAOQDO010001804         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91573_length_1548_cov_2.004689, whole genome shotgun sequence.
ACCESSION   DAOQDO010001804 DAOQDO010000000
VERSION     DAOQDO010001804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91573_length_1548_cov_2.004689"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKZ53_22795"
     CDS             <1..189
                     /locus_tag="PKZ53_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43332.1"
                     /translation="FTDAKVMTMALMQYYFRTPPLKQTFLLVKANDPGAFPRCCRYQQ
                     WIARLNRLIPLVAQDFGS"
     gene            296..874
                     /locus_tag="PKZ53_22800"
     CDS             296..874
                     /locus_tag="PKZ53_22800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNJ43333.1"
                     /translation="MTQHPPSQFTQLLSHWNHGDKNALNQIITLGYENLHQLARAFFR
                     REQAKNTLQPTILIHEVYLKMVHQTGLELKDRTHFFALAARMMRQILTDYARAKDAQK
                     RGGEIQFITLDQAVDFGTQVQIDFLALEEALEALAQLDPGQVEIVELRFYGGLSIEET
                     AEALGISPRTVNREWRLAKAWLYDRLFSDPVK"
     gene            complement(1076..>1548)
                     /locus_tag="PKZ53_22805"
     CDS             complement(1076..>1548)
                     /locus_tag="PKZ53_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43334.1"
                     /translation="NQLSEDARKYFEIVCLAGKPISRHVIQKAATIQHPEPLIVAELR
                     ARNLILIRQMDREHNIEVYHDRIREAVLKGISRDRRRALHCALAETLIFFNQGDPEQL
                     AIHYFEAERFGEALYYTIQAAELAEANLALDHATFLYQRADALRFLGKKPSDLD"
BASE COUNT          375 a          369 c          375 g          429 t
ORIGIN      
        1 tttaccgatg ccaaagtgat gacgatggcc ctgatgcaat actattttcg aaccccgccc
       61 ctcaaacaga ccttcctgct ggtcaaggcc aatgatccag gggcgtttcc cagatgttgc
      121 cgttaccaac agtggattgc acggttgaac cgattgatcc ctcttgttgc tcaagatttt
      181 ggatcatagt ttggttaccg ccgacttgat ccctgctttt tcaactcaag actgaggttt
      241 aattaagaaa acgaaatcca gttgaaaacc atgccaccag ttctgaaaat cttttatgac
      301 ccaacatcca ccttcacaat tcacccaact gctcagtcac tggaatcatg gtgacaaaaa
      361 cgcattgaat caaattatca cgttgggcta tgagaacctt caccagcttg cccgggcctt
      421 ctttcgtcgg gaacaggcca aaaatacgct tcagccaacg attcttattc atgaagtcta
      481 tttgaaaatg gtccatcaga ccggacttga actcaaagac cgaacccact tttttgcgct
      541 ggccgcccgc atgatgcgcc aaatcctgac tgattatgca cgggccaaag acgcccaaaa
      601 aagaggtggg gaaattcagt tcattacttt ggatcaggcg gttgattttg gtacccaggt
      661 ccagattgac tttctcgccc tggaagaagc cctggaggcc ctggcccagc ttgatcccgg
      721 tcaggttgaa attgtcgaac tccgcttcta cggtggtctc tccattgaag aaactgcaga
      781 ggccctgggg atctcgcccc ggaccgtcaa ccgtgagtgg cgattggcca aggcgtggtt
      841 atatgatcgg ctgttttcag acccggtcaa gtgactgcgc tgaccgggtg gattggtttt
      901 ggagttgtgc cttctcaaat aggtgccttg tcataaattt tctgagatat ttggatttgg
      961 gaagtatttg gtttttttcg tgtatttcgt gtgtttcgtg gttaaaaatg tctgagaatt
     1021 ttcggtaaag tacttattat ggtaaggctt aatgattgaa agaactctat tttcctcaat
     1081 ccaaatcgga cggttttttg cctaaaaacc gaagggcatc cgcccgctgg tataaaaaag
     1141 tggcatgatc gagtgccaga ttggcctcgg ccaattctgc tgcctgaatt gtgtagtaca
     1201 acgcttcacc aaatcgttca gcctcaaaat agtgaatcgc cagttgttcg gggtctccct
     1261 gattgaaaaa gatcaatgtt tcagcgagcg cacaatgcag ggctcggcgc cggtctcgac
     1321 tgatgccttt taaaaccgcc tctcggatcc ggtcatgata gacctcaatg ttatgctcgc
     1381 ggtccatttg gcggatcagg atgaggttcc gggcacgtaa ttcagccaca atcagaggtt
     1441 ctgggtgttg gatggtggcg gctttctgaa tgacgtgtcg actgatgggt ttcccggcga
     1501 gacaaacgat ttcaaaatat ttccgtgcgt cttctgaaag ttggttca
//
LOCUS       DAOQDO010001805         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91671_length_1547_cov_2.368633, whole genome shotgun sequence.
ACCESSION   DAOQDO010001805 DAOQDO010000000
VERSION     DAOQDO010001805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91671_length_1547_cov_2.368633"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..628)
                     /locus_tag="PKZ53_22810"
     CDS             complement(23..628)
                     /locus_tag="PKZ53_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43335.1"
                     /translation="MRALLLMLLIGSGILATSHIVFSQNKKSEEPLKSGIVYGYDHTF
                     AITAPDGWVLDNQAGVPDGLHAVFYPIGESWPNASTVMYTSVASKEKEHRSFEQIIDD
                     DVAGFREASGNGIAREIGKEKTADGAPVRIWYFENSKQGRIEQVAFIEESKVVVMLSY
                     SASSKKAYEKTSSALKDLLKSYRFLTDKVIIDKKPKDPTEK"
     gene            complement(677..1123)
                     /locus_tag="PKZ53_22815"
     CDS             complement(677..1123)
                     /locus_tag="PKZ53_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ43336.1"
                     /translation="MSIEIKILQPDEENLLNSVAPDIFDDPLDVERTREFLKDPRHHL
                     VVAIDQAQVVGFISAVHYVHPDKPNPELWINEVGVAPTHHGQGIGKALMKSLLNLARE
                     LNCSEAWVLTERTNLAAMKLYASAGGQEAPIDTVMFNFFLSDTSKE"
     gene            complement(1155..>1547)
                     /locus_tag="PKZ53_22820"
     CDS             complement(1155..>1547)
                     /locus_tag="PKZ53_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43337.1"
                     /translation="DKDTPIKQLAQTLATYLYPQEKKDFQWKELPSYGRLSKFEIADG
                     GGLQGFNGNQRVCLHYRKLLIRKQQVLVGYIFGLDRGAEAKKLFDQNLAGLSFSGVAA
                     QNHIIASMTDERFEDLSSGSAVRSNPIP"
BASE COUNT          385 a          358 c          353 g          451 t
ORIGIN      
        1 atttgtgtgg tgactggatg cttcattttt cagttgggtc tttcggtttt ttatcaatga
       61 tgactttgtc cgtgaggaat cggtaactct ttaacagatc tttgagagcc gaagaagttt
      121 tctcataggc ttttttgctc gaagctgaat aagagagcat caccacaact ttgctttctt
      181 cgataaaagc cacctgctct atccgacctt gtttggaatt ttcaaaatac cagattctga
      241 ctggtgctcc atcggctgtt ttctcttttc cgatttctcg ggcaatgcca ttgccgcttg
      301 cttctcgaaa accagccaca tcatcatcaa tgatttgctc aaacgaacgg tgctcttttt
      361 ccttcgaggc aactgaggta tacatcacag tggaagcatt cggccacgat tcaccgattg
      421 gatagaacac cgcgtgtaat ccatcgggaa cgcccgcctg attatcgagt acccatccgt
      481 caggggccgt aatggcaaac gtatggtcat acccgtagac aatcccggat tttaaaggtt
      541 cttcagattt tttgttttgg gaaaatacaa tgtgcgatgt ggccagaatt cctgacccga
      601 taagtaacat gagtagaaga gcacgcataa ttttctcttt tggtgataag ttgatcacaa
      661 gcgtgatttc cccagattac tctttggaag tatcgctgag gaaaaagtta aacatcacgg
      721 tatcaattgg cgcttcctgg ccgccggctg aggcataaag cttcatcgcc gcgaggttgg
      781 ttcgttcagt cagcacccag gcttcagagc agttcaattc acgagccaga ttcaaaagtg
      841 acttcatcag ggcttttcca attccctgac cgtgatgggt tggagcgacg ccgacttcgt
      901 tgatccacag ttccgggttg ggtttgtctg ggtggacata atgaacggca gaaatgaacc
      961 caaccacctg cgcctgatca atggcaacca ccagatgatg gcgcgggtct ttgagaaatt
     1021 cacgggtgcg ctccacatca agcgggtcgt caaaaatatc tggagcgaca ctgtttaaaa
     1081 ggttttcttc atctggttgc aggattttga tttcaatact catggtggat acctgttcag
     1141 gatgatgagg ggggttaagg aattggattg cttctaactg cgctccctga gcttaaatcc
     1201 tcaaatcgct catctgtcat cgaagcaatg atatgatttt gagcagccac acctgagaaa
     1261 gaaagtcccg ccagattttg atcaaacaat ttctttgctt ctgcaccgcg atctaaacca
     1321 aatatatatc ctacaagcac ctgctgcttc cgaatcagaa gcttgcggta gtgcaagcag
     1381 acacgctgat tgccattaaa cccttgaagc ccacctccat cagcaatctc aaatttgctc
     1441 aagcgtccat atgacgggag ctctttccac tgaaagtctt tcttttcttg tggataaagg
     1501 taggttgcaa gtgtttgggc cagttgtttt attggtgtgt ctttatc
//
LOCUS       DAOQDO010001806         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91747_length_1546_cov_2.935614, whole genome shotgun sequence.
ACCESSION   DAOQDO010001806 DAOQDO010000000
VERSION     DAOQDO010001806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91747_length_1546_cov_2.935614"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1546)
                     /locus_tag="PKZ53_22825"
     CDS             complement(183..>1546)
                     /locus_tag="PKZ53_22825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ43338.1"
                     /translation="QTWWASILYFVTVAGGLYGGHLYRLRRLHRQNAILEAKVIDRTA
                     QLDQKNEELAQTVVEVKSAKTEVEQKNKQLDAKIRELLDSQRQADRIFSALTEAMPGT
                     VIDEKYRLDEKIGSGGFGAVFRATHLALNRQVAVKVFRPTPGNDSADAIERFRLEGIS
                     ASRINHPHVVAILDSGITPDGIAYLVMELLNGHSLAQEMKELKVLTLRRCLEITVPIC
                     EALAKAHQMGVIHRDIKPENIYLHQAPNGEVVKLVDFGIAKLVEDELISSRHKLTLTG
                     GIIGTPAYMAPERLNNSKYDGKSDVYSLGIVVYEMLCGRTPFQNTANNLVEMVIFQLQ
                     RNPPKLHTVNPHIPLEIEAVVHQALDKDPQRRPTPTEFTEALAKAAANYPELLKEDTP
                     FPDMETDPLMIVDMPTQITHRSKLVAAMRVALKEPEADELPTRQVPPEEMVESGENSM
                     GHG"
BASE COUNT          349 a          402 c          362 g          433 t
ORIGIN      
        1 cctcgcatac attcagaatt gatcagtttt gattatacca ctaccatcta cttatatatt
       61 gttcactgat ttcaaatgac atttcccctc caagtcaacg acaaattaaa cataactaga
      121 agtctcccac taaccaaaga gtctaactgc cattatgccc tttatcttat ttttagccaa
      181 ttttacccat gccccatcga attttcaccg ctttcaacca tttcctctgg cggaacctgt
      241 cgtgtcggca attcatccgc ttcaggttct ttgagtgcca ctcgcattgc agccaccagt
      301 tttgaccgat gggtaatctg ggtcggcata tccacaatca tcaacgggtc agtttccata
      361 tctggaaagg gcgtatcttc tttgagcaat tccggatagt tggcggcagc ctttgccagc
      421 gcttcggtaa attcagtcgg agttggtcgc cgctgcgggt ctttgtccag tgcctgatgc
      481 acgacggctt cgatttcgag tgggatatgc ggattgaccg tgtgcaattt cggaggattg
      541 cgttgaagtt ggaaaatcac catttcaacc agattgttgg cggtgttttg aaacggtgtg
      601 cgaccacaga gcatttcata caccacaatg ccgaggctat agacatccga tttgccgtca
      661 tattttgaat tattcaaccg ttccggggcc atataggctg gcgtcccgat aattccgccc
      721 gtcagcgtga gtttatggcg gctcgaaatc aattcgtctt cgaccagttt ggcaatcccg
      781 aaatccacca gtttaaccac ttcgccattt ggggcctgat gcaggtaaat attttccggt
      841 ttgatgtcac gatggatcac acccatctgg tgggctttgg ccagtgcttc gcagattgga
      901 accgtgattt ccagacaccg ccggagcgtc agcactttca attctttcat ttcctgggcc
      961 agcgaatgcc cgttaagtag ctccatcacc agatacgcga taccgtccgg ggtaatgccc
     1021 gaatccagaa tggcgaccac gtgaggatga ttgatccgtg aggctgaaat gccttcgagg
     1081 cgaaaccgct cgatggcatc cgccgagtca tttccaggag ttggtcgaaa gactttcacc
     1141 gctacttggc gatttaatgc cagatgcgtg gctcgaaaca cggcgccaaa tccaccggac
     1201 ccgatttttt catccagccg gtatttttca tcaatcacgg ttccgggcat ggcttcggtc
     1261 agggctgaaa aaatccgatc agcctgacgt tgtgaatcga gcaattcgcg gattttggcg
     1321 tccagttgtt tgtttttttg ttcgacttcg gttttggcac ttttgacttc gaccacggtt
     1381 tgggccagtt cttcattttt ctgatcaagc tgggccgtcc ggtcaatgac tttggcttcg
     1441 aggatggcat tctggcgatg cagccttcgc aagcgataca gatgtccacc atacaaacca
     1501 cctgccacgg tcacaaaata caggatagaa gcccaccagg tctggt
//
LOCUS       DAOQDO010001807         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91764_length_1546_cov_2.659960, whole genome shotgun sequence.
ACCESSION   DAOQDO010001807 DAOQDO010000000
VERSION     DAOQDO010001807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91764_length_1546_cov_2.659960"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PKZ53_22830"
     CDS             complement(<1..1440)
                     /locus_tag="PKZ53_22830"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43339.1"
                     /translation="MLRAVCQSQRYIIYLGLLLFLSVWTLISVKLADAQTVQLSVVNG
                     SKSPLVTPGQTVHIWANVHLPGQVFERWEGNSELLADPLAAHTTLVMPARNVELIARF
                     KPAADWTPTEERINGSRVFSYFPQPHRGVITLYHGSGGSATSWTDGRGAPENLLFCRD
                     AVAAGFAIVATESQNRVDKQWNSRGTNNDDVENIQTILETFKARGEMRPDAPVFAVGM
                     SNGGGFSPLVSALLGFRATAIFCAAGYDAVIEQTQVPTIWCLAENDQIVNNSDAVTNF
                     GVLSGRKVPAELYTNGASPVFPLRFARIEGMSEADSTEFYTRLKNNGFLDDQDFLESS
                     PAASNWERLLGEYTPFRNGIEDELNVCRASHKFFSDFNHKVLLFFQRAATGDTESPVV
                     SEVRTSKGKIIRKKDATVTITWRSTDNVGVESQDLAFAGDGATFTTPVASGLTGSTQS
                     FSWSIPTNLAKTKTGRLRVLARDTAGNSGE"
BASE COUNT          357 a          419 c          403 g          367 t
ORIGIN      
        1 ttcaccgctg ttacccgctg tgtcccgagc gagcactctc aaccggcctg tcttggtttt
       61 tgccagattg gtggggattg accaggaaaa cgactgtgtg cttcctgtca atccagaagc
      121 cacaggtgtg gtaaaggttg ccccgtcccc ggcaaaggcc agatcctgag attcaacccc
      181 aacattgtca gttgagcgcc aggtaatagt caccgtggca tcttttttgc ggatgatttt
      241 tccttttgag gtgcgaacct ctgacacaac cggactttcg gtgtctccgg tggcggctcg
      301 ctggaaaaag agcaggactt tatgattgaa gtcgctgaag aacttatggc tggcacggca
      361 tacattcaat tcgtcttcga tgccgttgcg aaacggggtg tactcgccca aaagccgctc
      421 ccagtttgag gcggcaggcg atgattccag gaaatcctgg tcatcgagga acccgttgtt
      481 tttgagccgg gtgtagaatt cagtcgaatc agcttcgctc atgccttcga tccgggcaaa
      541 ccgaagcgga aatactggtg acgcaccgtt ggtgtacagt tcggctggaa cttttctgcc
      601 gctcaggacg ccaaaattgg tcacggcatc gctgttgttc acgatctgat cattttcagc
      661 cagacaccag atggtcggaa cctgggtttg ctcaatcacc gcgtcgtacc cggcagcaca
      721 aaaaatggca gttgctcgaa acccaagcaa cgcagacaca agcggtgaaa acccgccgcc
      781 atttgacatt ccaacggcaa acactggggc atcaggtcgc atttcgccac gcgctttgaa
      841 cgtttctaaa atggtctgga tattttcgac atcgtcattg ttggtcccac ggctattcca
      901 ttgtttgtcc acccgattct ggctttcagt tgccacaatc gcaaatccag ccgccaccgc
      961 atcgcgacaa aagagcagat tttccggggc accacgacca tcagtccaac tggttgcact
     1021 cccgccgctc ccgtgataga gcgtaatcac accacgatgg ggttggggaa aataagaaaa
     1081 cacccggctg ccgttgatcc gctcttcggt tggtgtccag tcagccgctg gtttgaagcg
     1141 ggcgatcagt tcaacatttc tggccggcat caccagtgtc gtatgggcgg ccagagggtc
     1201 ggccagcagt tccgaatttc cctcccaccg ctcaaacacc tgtccaggca ggtggacatt
     1261 tgcccagata tggacggttt gcccaggtgt cacgagtggg cttttgctgc cattgacgac
     1321 cgaaagctga acggtttgtg catcggccaa tttcaccgaa atcagcgtcc agacactcag
     1381 aaacaacaga agaccgagat aaataatgta cctctggctt tgacaaacag cgcgcaacat
     1441 tttgaacatc cttcttttat ggtttgagtc gagttgaaaa atgttttcag gcagggccag
     1501 ggggagtctg cacctgagac gtcttgtcca gtcaaaaagt cgtgca
//
LOCUS       DAOQDO010001808         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91865_length_1545_cov_2.971141, whole genome shotgun sequence.
ACCESSION   DAOQDO010001808 DAOQDO010000000
VERSION     DAOQDO010001808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91865_length_1545_cov_2.971141"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKZ53_22835"
     CDS             <1..747
                     /locus_tag="PKZ53_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNJ43340.1"
                     /translation="QTGTRIQHLILDTSVIIDGRVADIAETGFLAGRLVIPQFVLREL
                     QQVADSADSAKRNRGRRGLDILQRIQKNPNLDITITDVDFPDIREVDLKLIELAKQLD
                     GKIVTNDFNLNKVSQLRGVPVLNINELANALRPVVLPGEIMRVFILKEGKEYNQGVAY
                     LDDGTMVVVDNARRNIGKTIDVNVTSVLQTTAGKMIFGRFNDESSIRPEASIPPRDPR
                     EARMDRTERSERPERLNAPRPITPTPSPKA"
     gene            808..1527
                     /gene="ispD"
                     /locus_tag="PKZ53_22840"
     CDS             808..1527
                     /gene="ispD"
                     /locus_tag="PKZ53_22840"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNJ43341.1"
                     /translation="MNIALVPAGGSGTRFGAVKAKQFLELAGIPIIIRTLRHLCDCPD
                     ISGVIVALPESDRLQFQELLAQFPLPKPCQSVIGGRERQDSVFAALEAAPVETRYFVV
                     HDAVRPFISAQLVSATLVAAQEDRAAIVGYPVTDTIKLVENGVAVSTPPRNTLFAVQT
                     PQTFEAGLLREAHNRANHEGWRATDDAMLVEGLGVPVRIVPGPLDNLKITHPRDLELA
                     EFVLKQQERMKNEELRMKNEE"
BASE COUNT          398 a          393 c          404 g          350 t
ORIGIN      
        1 cagactggaa ctcgaattca acacctgatc cttgacacca gtgtgatcat tgatggtcgg
       61 gtggcggata ttgcggagac tgggtttctg gctggccgac ttgtgattcc gcaatttgtg
      121 ctccgcgaac tccagcaggt tgccgatagt gctgactcag ccaagcgcaa tcgtggccgg
      181 cggggactcg acattctgca acgcattcag aaaaacccca accttgacat taccatcaca
      241 gatgttgact tccctgatat tcgcgaagtt gatctcaaat tgatcgaact ggcgaaacag
      301 cttgatggaa agattgtcac caatgacttt aacctcaata aagtctctca attgcgtggc
      361 gttccagtgc tcaacatcaa tgaactggcc aatgccctgc gtccggtggt gctccctggc
      421 gagattatgc gggtgtttat tctgaaagaa ggcaaagaat acaatcaggg tgtcgcctat
      481 ctggatgacg gcaccatggt ggtggttgac aatgcccgcc ggaacattgg caaaaccatt
      541 gatgtcaacg tcaccagcgt gctgcaaaca acggctggaa agatgatttt tgggcgcttt
      601 aacgacgaga gcagcatccg ccccgaagct tcaattccac ctcgtgaccc acgtgaagcc
      661 cggatggatc gaactgaacg ttccgaacgc ccggaacgac tcaatgcacc acgaccaatc
      721 acgccaacgc cttcgccgaa agcctaaaaa taccagggct tggggcttgg ggctgaagat
      781 tctgggtttt caaaaaccag gtactgaatg aatattgcac ttgttccggc aggcggctct
      841 ggtacgcgct ttggagccgt caaagccaag cagtttcttg aactggcagg aattccgatc
      901 attattcgca ccctgcgtca cctttgtgac tgccctgaca tttcaggggt gattgtggcg
      961 ttaccggaaa gtgatcgcct gcaatttcaa gagcttctgg ctcaattccc acttcccaaa
     1021 ccctgtcagt cagtgatcgg tggacgcgaa cgccaggatt cggtgtttgc ggctcttgag
     1081 gcggcgccag tggaaacacg ctattttgtt gttcacgacg cagtccgccc atttatttca
     1141 gcccaactgg tttcagcaac cctggtggca gcccaggaag acagagccgc catcgtgggc
     1201 tatccggtca ccgatacgat taaactggtt gaaaatggag tcgcggtttc cactccgcca
     1261 agaaataccc tgtttgccgt tcaaacccca caaacatttg aggctggtct actccgtgaa
     1321 gcacacaacc gggccaatca cgaaggctgg cgggcaactg atgatgcaat gctggtcgaa
     1381 ggtctgggtg taccggtgag aattgtgcca ggaccgcttg ataacctgaa gattacccat
     1441 ccacgtgacc tggaactggc agaatttgtg ctgaagcaac aggaacgaat gaagaatgaa
     1501 gaattgagaa tgaagaatga agaatgaaga ataaagaatg aagaa
//
LOCUS       DAOQDO010001809         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_91949_length_1544_cov_3.832102, whole genome shotgun sequence.
ACCESSION   DAOQDO010001809 DAOQDO010000000
VERSION     DAOQDO010001809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91949_length_1544_cov_3.832102"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKZ53_22845"
     CDS             <1..274
                     /locus_tag="PKZ53_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43342.1"
                     /translation="LHLSGHVEAFVCAITPDNLHYIIRKEKAKQVGPDPSQHHVSLQF
                     AHQVISDVVTTLLVAPVDKSVLQAAVALIGRILKTRYRVPVPQSYL"
     gene            complement(440..1441)
                     /locus_tag="PKZ53_22850"
     CDS             complement(440..1441)
                     /locus_tag="PKZ53_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ43343.1"
                     /translation="MRRLIILVLLSSLFLSLACKTGPSPEEAGKIRVGIVFDIGGKDD
                     KSFNAAAWEGVKRAAKDLPIVLRDVEPGEPTSLEPSMRAFAERGYDLIIGIGFAQAPI
                     MESVAKDYPNLNFAIVDSVIELPNVASLVFDEHEGCFLVGMIAARTTKTNKIGFVGGM
                     DIPLIRKFAKGYEEGIHYVNPKCEVYENFVGINDAAWNNPGKGKELAKAQFDKGADIV
                     FQAAGNSGIGVFDAAEEVNRFAIGVDSNQNWMKPGIILTSMVKRVDVAVYSTVKEVVE
                     KKFKAGIHSYGLQNDGITFALDQYNQKLIPQTVIDEVDAARQKIIKQEIKVTNIMAK"
BASE COUNT          388 a          388 c          351 g          417 t
ORIGIN      
        1 tcttcattta tccggccatg ttgaggcttt tgtctgtgcc atcacgcctg ataatcttca
       61 ttacataatt cgaaaagaaa aggccaaaca ggtaggccct gacccttctc aacaccatgt
      121 atcgcttcag tttgcacatc aggtgatttc agatgtggtc accactttgc tggtcgcccc
      181 ggttgataaa tccgttttgc aagctgccgt tgctttaatt ggaaggattt tgaagacgcg
      241 atacagagtg cctgtgccac agagttatct ttagacgcga ttgtcactcg cgataagaag
      301 gattttcagt caccgtcggt acaaattttt cagccggacg agtttctgaa ctttctggcc
      361 aatcagtacc ccgaaccacc acagaaccaa taagttatct catcattaaa cgtaactcag
      421 aacatattct ctgagaaagc tacttcgcca taatgttggt gactttgatt tcttgcttga
      481 tgattttctg gcgggcggca tccacttcgt caatcaccgt ttggggaatc aatttctgat
      541 tgtattgatc aagggcaaaa gtaataccat cgttttggag tccgtaggag tgaattccgg
      601 ctttgaattt cttttcgacg acttctttga ccgtgctgta cacagcgaca tccacgcgtt
      661 tgaccatgct ggtcagaata atgcccggct tcatccagtt ttgatttgag tcaacgccaa
      721 tggcaaaacg gttaacttct tcagccgcat caaagacccc aatccccgag tttccagccg
      781 cctgaaagac gatatccgca cctttgtcaa actgcgcttt ggctaattcc ttgcctttgc
      841 cggggttatt ccaggcagca tcgttgatgc cgacgaagtt ttcatagact tcgcactttg
      901 gattgacata gtggatgcct tcttcatagc ccttggcaaa tttacggatc aacggaatgt
      961 ccatgccacc gacaaaccca attttgttgg ttttggtggt tcgggcggca atcattccca
     1021 ccagaaagca gccttcgtgt tcgtcaaaga ccagggacgc cacgttgggc agttcaatca
     1081 ctgagtccac aatcgcaaag ttcaaattcg gatagtcttt ggcgacgctt tccataatcg
     1141 gcgcctgggc aaacccgata ccgataatca ggtcatatcc acgttcggca aaggcccgca
     1201 tggagggctc cagcgacgtg ggttcaccgg gttcaacatc ccggagcaca atcgggaggt
     1261 ctttcgccgc ccgtttgaca ccctcccagg cggcggcatt aaacgactta tcgtcttttc
     1321 cgccaatatc aaagacaatt ccgacccgaa ttttcccggc ttcttccgga cttggaccag
     1381 ttttgcacgc cagggacaga aacaaagaac taagcagcac caggatgatc aaacgtcgca
     1441 taaaacccca ttgagaatga agaattgaga atgaagaatt gagaatgaag aaagaagaaa
     1501 gcttagtgct tggtgcttgg tgcttggtgc ttagtacttg gtgc
//
LOCUS       DAOQDO010001810         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92022_length_1544_cov_1.850907, whole genome shotgun sequence.
ACCESSION   DAOQDO010001810 DAOQDO010000000
VERSION     DAOQDO010001810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92022_length_1544_cov_1.850907"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1544)
                     /locus_tag="PKZ53_22855"
     CDS             complement(<1..>1544)
                     /locus_tag="PKZ53_22855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ43344.1"
                     /translation="NTDLLQRVLSEERMFDAEYRIQRADGVYRWFKVRAVPFRDQSGK
                     IVRWIGTSTDIDDQKQTEVALRQWADAFENCAHGIAIGDPVTNCVIACNPAFASLHHQ
                     SAEGIVGQPLLSLYDPADWDYIKACIASADQIGQVRYETRMQRKDGALFNVQMDVVSV
                     RDESGRVRYRVATMQDITDRKQAEEIIRESEARYRNLVEVSPDAIFINRDRQIVFVNR
                     QGLKLFGAQTSDQLLGKSPYDLFHPDYHELIRHRIQTMLATKKSSRAIEEKIVRLDGQ
                     VVDVEVTASSFLDQEKQAIHVVLRDITERKQAEHRLQTQARVSRILAEANTFGETALD
                     IIQTLCEAEAWDYGVIWKVDKKANQLECVDVWARPEPGLEALVAQTRSLTFAPNMCLP
                     GRVWIKGKAAGDEAVVGEHHLCPRAPLAIKSGLCSTVAFPIVHQGETLGVMDFMSRTR
                     IVLDQPLQAQLGIVGSQLGQFLARNQAQEEVHRFVSGSPAVIFALKREEAAWKPAWTS
                     ENVFDL"
BASE COUNT          359 a          445 c          380 g          360 t
ORIGIN      
        1 tcagatcaaa cacattctca ctggtccagg ccggtttcca ggctgcctct tctcgcttga
       61 gggcaaagat aacggctggg cttcctgaaa caaaccggtg gacttcttcc tgggcctggt
      121 tgcgagccag aaattggcca agctgacttc cgacaatacc cagttgcgcc tggagcggct
      181 ggtcaaggac aatccgagtt cggctcataa aatccataac cccaagcgtt tccccttgat
      241 gcacaatcgg gaacgccacc gtgctgcaga gaccagattt gattgccaat ggtgcccggg
      301 gacagaggtg atgctcaccg acaacagcct catctccagc cgctttgcct tttatccaaa
      361 cccgtcctgg caaacacata ttaggggcaa atgtcaggga ccgggtctga gcgaccaggg
      421 cttcaagtcc aggctcagga cgtgcccaaa catcaacaca ctccaactgg ttcgcctttt
      481 tatcaacttt ccaaatcaca ccatagtccc aggcttcggc ttcacataag gtctggatga
      541 tgtcgagtgc ggtttcgcca aaggtattgg cttcagccaa aatacgactg accctggcct
      601 gggtctgcaa gcggtgctcg gcctgcttcc gctctgtaat atcgcgtaag acaacgtgaa
      661 tcgcctgttt ctcctgatcc agaaacgatg atgcggttac ttcgacatca accacctgtc
      721 cgtccaggcg gacaattttt tcttcgatag ccctggacga tttttttgtg gccagcatgg
      781 tttgaatccg atgacggatc aactcgtgat agtctgggtg aaagagatca tagggagact
      841 taccaaggag ttgatcactg gtttgggccc caaacaactt cagcccctgg cgattcacaa
      901 agacaatctg gcggtcacga ttgataaaaa tggcatctgg cgaaacttcg accagattgc
      961 ggtagcgggc ttcgctttcc cggatgattt cctcagcctg tttccggtcg gtaatatcct
     1021 gcatcgtcgc cacccgatat cgaacccgcc cgctctcgtc acgcacagac accacatcca
     1081 tctggacatt gaacagagcg ccatctttgc gctgcattcg ggtttcgtag cgtacctgcc
     1141 caatctggtc ggcgctggca atacaggctt tgatgtaatc ccaatcagcc gggtcataca
     1201 gactcaggag tggttgtccc acaatgcctt ccgccgattg atgatgcaac gaagcaaaag
     1261 ccgggttaca ggcaatgacg caattggtga ccggatcacc aatcgcgatg ccatgcgcac
     1321 aattttcgaa ggcgtcagcc cactggcgca gggcgacttc ggtttgtttt tgatcgtcaa
     1381 tatcggtact ggtgccgatc catcgcacga tttttcctga ctgatcacgg aacggaaccg
     1441 cccgcacctt aaaccagcga tacaccccat ctgcccgctg gatccgatat tccgcatcaa
     1501 acatccgttc ttcagagagc acccgctgaa ggaggtcggt attg
//
LOCUS       DAOQDO010001811         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92101_length_1543_cov_2.520833, whole genome shotgun sequence.
ACCESSION   DAOQDO010001811 DAOQDO010000000
VERSION     DAOQDO010001811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92101_length_1543_cov_2.520833"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..>1543)
                     /locus_tag="PKZ53_22860"
     CDS             complement(12..>1543)
                     /locus_tag="PKZ53_22860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43345.1"
                     /translation="DDLENLHAQFQSLVTPWDDAFISIDDHQTIVWVSPGAEKMFGYT
                     AEELIGTPADRLTPKRFLETHHEYINRMLKSPESVTPKEKPQRVWGLRKNQAEFPGEA
                     FIAKSIRNHKTILTIRLHDLSEQLHLEEQFLQAQKMEVVGRLASGVAHDFNNLLTVIL
                     GHSNLLLRWSGLDTTAYRSLKEITKAAERASELTRQLLLFSRKQEQELKIIHLNTVLT
                     DINKMLQRIIGEDIELVYDLDPTIQAIKADPRHIEQIVLNLCINARDALPQGGTIHIS
                     THLYQSDFVPIGSYASLLPGSYIALKIQDTGTGIRPELLDRIFEPFFTTKDPEKGTGL
                     GLSTVYGLVRQSEGQITVESELGKGTTFSVFLPCNEETLDLIPAEPILTTTAQGTETI
                     LVVEDDKMVSEYLKQVLEMSGFQVLVAQNGMEAHKICMHTLKPLHLLMTDVVMPKMNG
                     IELAKMVSRFHPNMKILIMSGYQDQVQDTRTLHPSWAFIRKPFRVEELLKKIRDLLDA
                     D"
BASE COUNT          357 a          373 c          378 g          435 t
ORIGIN      
        1 cctaaatggt attagtcggc gtccagcaaa tccctgattt ttttgagtaa ttcttctacc
       61 cggaacggtt tgcggataaa tgcccagctt gggtgcagcg ttcgagtatc ctgaacctgg
      121 tcctgatagc ccgacatgat caaaatcttc atgtttgggt gaaatcggct caccatttta
      181 gccagttcga ttccattcat tttgggcatg accacatcgg tcatcagcaa atggagtggt
      241 ttcagggtgt gcatacagat tttgtgggct tccatcccat tttgtgcgac cagaacctga
      301 aacccactca tttccaaaac ctgcttcagg tattcactta ccattttgtc atcttcaacc
      361 accagaatgg tttcagttcc ttgagccgtc gtggtgagaa tcggttcggc ggggatcagg
      421 tccagagttt cttcattaca gggcaaaaat accgagaatg tcgttccttt tccgagttca
      481 ctttcaaccg taatctggcc ttcgctttgc cgaaccaatc cgtacacggt tgagaggcca
      541 agtccggtcc ctttttcggg gtctttggtc gtaaaaaatg gttcaaaaat tcggtccagc
      601 agttcaggcc gaatcccggt gccggtgtcc tggattttga gggcaatata gctgcccggc
      661 agcaatgacg cataactgcc aatcggaaca aaatcagatt gatacagatg agttgaaata
      721 tgaatggtgc caccctgtgg cagggcatct cgggcgttga tacacaaatt caggacaatt
      781 tgctcaatgt ggcgtgggtc agccttaatg gcctgaatgg ttggatcaag gtcatacacc
      841 agctcaatat cctcgccaat gatccgctgg agcattttat tgatgtcagt gagaactgta
      901 ttgaggtgaa tgattttgag ttcttgttcc tgcttccggc taaaaagcag caattgccgg
      961 gtgagttccg acgcccgttc ggcggccttg gtaatttctt tcaggctgcg ataggccgtg
     1021 gtgtctaacc cggaccaccg caacagcagg ttgctatgac ccagaatgac cgtcagcaga
     1081 ttattgaaat catgcgccac cccgcttgcc agtcggccca caacttccat tttttgcgcc
     1141 tgaaggaatt gttcctcaag gtgtaattgc tcagacaaat catggagccg aatcgtcaaa
     1201 attgttttgt ggttccggat tgatttggca atgaacgcct cacctggaaa ttccgcctga
     1261 tttttgcgca gcccccaaac ccgctggggt ttttcttttg gagttactga ctcaggcgat
     1321 ttaagcatgc ggttgatata ctcatggtga gtttccagaa accgttttgg cgtcagtcta
     1381 tcggctgggg tgccaatcaa ttcttcggca gtgtatccaa acatcttttc tgcccctgga
     1441 ctgacccaaa caatagtctg gtggtcgtca attgagataa aggcgtcgtc ccagggggtc
     1501 accagacttt gaaattgggc gtgaaggttc tctaagtcat cct
//
LOCUS       DAOQDO010001812         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92300_length_1541_cov_3.273217, whole genome shotgun sequence.
ACCESSION   DAOQDO010001812 DAOQDO010000000
VERSION     DAOQDO010001812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92300_length_1541_cov_3.273217"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..>1541
                     /locus_tag="PKZ53_22865"
     CDS             343..>1541
                     /locus_tag="PKZ53_22865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ43346.1"
                     /translation="MSSPQPASHDGFSALTRNVRRTHLPNGLTILTKEVHTHPIVAAM
                     IWYRVGSRNEELGHTGKSHFLEHMMFKGTERLGKGEIDLLTQIRGGRNNAFTWLDFTA
                     YYFTFASDRWEMALEIEADRIRNTSFAPEEFESEKQVIIEELQMGLDSPFELLENEVW
                     ATAFRQHPYHSPTVGWLQDVQGVTVEDMKRYYDQWYQPRNATVVLVGDFETEQAIARV
                     TELFGHLPAGPEPPAMKIVEPPQRGEKRVIVKKITPVERLMIAYHAPEIAHSDSYALH
                     VASMLFSHGRTSRLYQRLQEKDQSVTMAYASYNEHIDPSLFYIQAEIKSGHTLAAVEQ
                     AIYEEIDRLATEPIPDAELKKAKRQIEAMYVLGNEDVLNQATLLGEYEPTANRRNSLK
                     KNVATVI"
BASE COUNT          402 a          431 c          380 g          328 t
ORIGIN      
        1 tggtcaaatg tcacggattc cgtttgagcg aatcccacac ttcccccacc caatgaccaa
       61 aggatgacac acaggagtaa acaaattggt acacgcataa cagtttggga agacagtggg
      121 ggaaagggtt gcggccaggc gcgtcgaaga aagttggaac gatcagacca gcacgtgagc
      181 ggctctgacc aggcacgcga ttgtacctga atctcaccag ggttgcaccc gactccctcc
      241 gccgagaaag cattgtctcc catcaaattc ccctctatac tggctggctt ttctcccaaa
      301 agctgaaatt cggccaatct ccaaaaggag caccacgttc gtatgtccag cccacagcct
      361 gcttcacatg atggattttc ggcactgact cgcaatgtcc gccgtacaca tctacccaat
      421 ggcttaacca ttctcaccaa agaagtccat acccatccca tcgtagcggc gatgatctgg
      481 tatcgagttg gctcccgcaa cgaagaattg ggccataccg gcaagtctca tttcctggag
      541 catatgatgt ttaaagggac tgagcggctt ggcaaaggcg agattgacct gctgacacag
      601 attcgtggcg ggcgcaacaa cgccttcacg tggcttgatt tcaccgccta ttacttcacc
      661 tttgcctcag accggtggga aatggcgctc gaaatcgagg ctgaccgcat ccgaaacacg
      721 tcgtttgccc cggaagaatt tgaatcagaa aaacaggtca tcattgaaga actccaaatg
      781 ggcctcgata gcccgtttga attgctcgaa aatgaagtct gggccaccgc gtttcgacaa
      841 catccgtatc attcgccaac ggttggctgg ttgcaggatg tgcaaggcgt cacggttgag
      901 gacatgaagc gctattacga ccagtggtat cagccgcgca atgccaccgt ggtgctggtt
      961 ggtgattttg aaaccgaaca ggcaattgcc cgtgtcaccg aactctttgg acatctgccc
     1021 gctggtccgg aaccgcccgc catgaaaatc gttgaaccac cccaacgcgg tgaaaaacgg
     1081 gtcattgtca agaaaatcac tccggtggag cggctgatga ttgcctatca cgccccagaa
     1141 attgcccact ccgactcata tgccctgcac gtagcctcga tgttattttc ccacggacga
     1201 acatcacggc tctatcaacg ccttcaagaa aaagaccagt ccgtgaccat ggcctatgcc
     1261 agctacaacg agcacattga tccttcactg ttttatattc aggccgaaat taaatccgga
     1321 cacaccctgg cagcggtgga acaggcgatt tatgaagaga ttgaccggct ggcaaccgag
     1381 ccgattcccg acgccgaact aaagaaagcc aagcgccaga ttgaagccat gtatgtgctt
     1441 gggaatgaag atgtgctcaa tcaggcgact ttgcttggcg aatatgaacc cacggccaat
     1501 cgccggaact ccctgaagaa gaacgtggct accgttatct g
//
LOCUS       DAOQDO010001813         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92315_length_1541_cov_2.697174, whole genome shotgun sequence.
ACCESSION   DAOQDO010001813 DAOQDO010000000
VERSION     DAOQDO010001813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92315_length_1541_cov_2.697174"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /locus_tag="PKZ53_22870"
     CDS             complement(<1..>1541)
                     /locus_tag="PKZ53_22870"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43347.1"
                     /translation="HKVTQDVYTARDGSIWVNTFGGYAVIRNGKISSFPGSIDLLNRH
                     SVMFGENEQGLLAFTPGVGMVRVTSSEAVRISLASEFDALADNQRSWLFQDRDGTLWG
                     ATLGKLHHLVNGKVRTYTVADGLLGLQIGVMYRDRDDNLWMGFEGGGLGRLTAGQFTY
                     FSQKDGLTNDLVRTICEDQEGNLWVGTTGGGLNCFKDEKFTVYNKRHGLADNNVRVVL
                     EDRQGNLWIGTNGNGLNCLKPDRSVVTYTVENGLLNNFIRSLAEDHTGAIWVGFNGSG
                     VARIANGQVTSFQGQPGLAQQPVYTILEDHHGTMWFGSHGGGLTRYENGTFRSYTTED
                     GLAHNTVVALVEDNIGDGLWVGSFLGLHYFKDGKFRVWHSQDGMPDETVRSLTVDAAG
                     TVWVGTNGGLARLKNGRMIPITTSNGLADNAVFSILEDQSGYLWMSCNRGISRVRKQD
                     IEDFCDGKRPSFTHDAFTRDDGMRNNQCNGIAQPAAIRGHDGRFWFAGVAGLITINPD
                     HIRRNP"
BASE COUNT          362 a          469 c          374 g          336 t
ORIGIN      
        1 ggattccgcc ggatgtgatc cgggttaatt gtgatcaggc cagctacgcc ggcaaaccag
       61 aaccgtccat catgcccgcg aatcgccgcc ggttgggcaa tcccgttgca ctggttgttg
      121 cgcatgccat cgtcacgagt gaaggcgtca tgggtaaatg atggacgctt gccgtcgcaa
      181 aaatcctcga tgtcctgttt gcgtacccgc gaaatgcccc ggttgcaact catccaaagg
      241 tatccgctct ggtcttcaag aattgaaaac acggcgttat ccgccagccc gttgctggtt
      301 gtgatcggga tcatccgccc atttttgagc cgggccagtc cgccattggt gccgacccag
      361 acagtgccag ccgcatcaac cgtcagcgaa cgaacggttt catcgggcat tccgtcctga
      421 gagtgccaga cgcgaaactt cccgtctttg aaatagtgca ggcccagaaa tgacccaacc
      481 cacagaccat cgccgatgtt gtcttccacc agagccacaa ctgtgttgtg cgccagacca
      541 tcctcagtgg tgtaactccg aaacgtaccg ttttcatatc gggtcaggcc accaccatga
      601 gagccaaacc acatcgtgcc gtggtggtcc tccagaatgg tatacactgg ttgttgtgcc
      661 aacccaggct gcccctgaaa cgatgtcacc tgaccattag caatccgtgc cacacccgaa
      721 ccattgaacc caacccagat ggccccggtg tggtcttcgg cgagtgagcg aataaaattg
      781 ttaagcaacc cattttcaac cgtataggtc acgactgacc gatcaggctt gaggcaattg
      841 agcccattgc cgttggtgcc gatccacaaa tttccctggc gatcttcaag caccacccgc
      901 acattgttat ccgccagccc atggcgcttg ttgtagacgg taaacttctc atctttaaag
      961 cagttgagcc cgccgccagt ggtaccgacc cagagattgc cttcctgatc ctcacaaatg
     1021 gttcgcacca gatcattggt caggccatct ttttgagaaa agtaggtaaa ctgtccagcg
     1081 gtcaatcgtc ccaaaccacc gccttcaaat cccatccaca aattgtcatc ccgatcccgg
     1141 tacataacgc cgatttggag ccccagcaga ccatcagcaa cggtgtaggt ccggactttt
     1201 ccattgacca gatgatggag tttccccagg gtcgcccccc acaatgtgcc atcgcggtcc
     1261 tgaaacagcc aggaccgctg attgtcagcc agggcatcaa attcactggc caatgaaatc
     1321 ctgaccgctt cggaagaggt gacccgcacc atgccaacac ctggcgtaaa cgccaggagt
     1381 ccctgctcgt tttccccaaa catcacggaa tgtcggttga gcaaatcaat cgagccagga
     1441 aatgaggaga ttttcccgtt tcggatcacg gcgtatccgc caaaggtgtt gacccagata
     1501 gagccatccc gtgcggtata tacatcctgg gtgaccttat g
//
LOCUS       DAOQDO010001814         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92543_length_1539_cov_2.547844, whole genome shotgun sequence.
ACCESSION   DAOQDO010001814 DAOQDO010000000
VERSION     DAOQDO010001814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92543_length_1539_cov_2.547844"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..>1539
                     /locus_tag="PKZ53_22875"
     CDS             134..>1539
                     /locus_tag="PKZ53_22875"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNJ43348.1"
                     /translation="MNGNFTRISFPDVIRELHLTRRTGLLRLVQDKTLRAIFVEEGKL
                     VFALSNLPQERLGDFLLRENRITREQYDQALLHPNSKQRMGKVLVELGFLRPDEADFY
                     TKRQITEIILANFGWSHGEFTFEENTRAAHDVKLDLLTPNIILMGVRTITDENLIRSA
                     VGPVAQKVQMSMEAPLQLMQATLDGTEGFLLSRITDSHSLDELVTMCGLPEAMVLRVI
                     YGLICAGILESDYPRPMMDGSRPASIHSQAAPAGASATPPPPSKSSAAATIPVSGPNT
                     GSQPPLSTPSSAEPVLTEKDARDDLKKILYINSLKTTTYYTVLGVDPSAREADIKKAY
                     YALAKKYHPDRFRQYGAADILTDAEQVFAKIGEAYEKLKDLDFRRRYDDFLGVGASSS
                     TSGMSAAPAPPPTPPSTHNPYKAASTPSSSASPSTSSSGHGSSGFGKPMSPPPSTSSS
                     GHGSSGFGKPMSPPPSTS"
BASE COUNT          373 a          434 c          373 g          359 t
ORIGIN      
        1 ctgcaatcaa ggttttggct caatcggaac ggatattgaa ggtttcattg acagtcaaac
       61 aggcggtcgt tagagtttga agacgacacg tcagacggcc ctcccttttc agttcatctt
      121 tggaatcagt tgtatgaatg gtaatttcac ccgaatttca ttccccgacg tcattcgcga
      181 gctgcatctt acacgccgca caggattgct tcggcttgtt caagataaaa cattgcgtgc
      241 catttttgtt gaagaaggga aactggtgtt tgccctttcc aacctgccac aggaacgatt
      301 aggagatttc ctgttgcgtg aaaaccgcat tacccgcgaa caatatgacc aggccctgct
      361 ccacccgaat agtaaacaac ggatgggtaa agtcctggtt gaattggggt ttttgcgtcc
      421 tgacgaagct gatttttaca ccaaacgcca gattacagaa atcatcctgg ccaattttgg
      481 ctggagccat ggtgaattta cctttgaaga aaatacccgt gctgcccacg acgtcaaact
      541 cgatttgtta acaccaaata ttatcttgat gggtgtgcgc accattacgg atgaaaactt
      601 gattcggagt gcggtgggcc cggtcgccca aaaagttcaa atgtcaatgg aggcgccgct
      661 ccaactcatg caggccaccc tggatgggac cgaaggtttt ttactctcgc gcattaccga
      721 ttcccattct ctcgatgaac tggtgacgat gtgcggcctg ccggaagcga tggtgttgcg
      781 ggttatttat ggcctgattt gtgccggaat tttagaaagc gattatccgc gaccgatgat
      841 ggacggcagt cgcccggctt cgattcattc tcaggcagcg ccggctggcg cctcagcaac
      901 gccaccaccg ccgtcaaagt ccagtgccgc tgcgaccatc cccgtttcgg gaccaaacac
      961 gggctcgcaa cctccacttt caacgccatc gtccgccgaa cccgtcctga cagaaaagga
     1021 cgcccgtgac gatctgaaga agatcctgta tatcaattca ctcaaaacca cgacctatta
     1081 cacggtgctt ggtgttgacc cgtcagcccg cgaagccgat atcaaaaaag catattacgc
     1141 cctggccaaa aaataccatc cagaccgatt tcgccagtac ggagcggctg atattttgac
     1201 ggatgctgag caggtctttg ccaagattgg cgaagcctat gaaaaattga aggaccttga
     1261 tttccgccgc cgctacgatg attttctggg tgtgggggcg agtagcagca cctctgggat
     1321 gtccgccgca ccagctccac cgccaacacc accatcaacg cacaacccat acaaggccgc
     1381 ctcaactccg tcaagcagtg cttccccctc cacttcaagc tcaggtcatg gctcaagcgg
     1441 ttttgggaaa ccgatgtcac cgccgccctc aacctcaagt tctggccacg gctcaagcgg
     1501 ttttgggaag ccgatgtcac cgccgccctc aacctcaag
//
LOCUS       DAOQDO010001815         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92644_length_1538_cov_2.469993, whole genome shotgun sequence.
ACCESSION   DAOQDO010001815 DAOQDO010000000
VERSION     DAOQDO010001815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92644_length_1538_cov_2.469993"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PKZ53_22880"
     CDS             complement(<1..1215)
                     /locus_tag="PKZ53_22880"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43349.1"
                     /translation="MKTIRPVVVVGVLACCFGPGVFQTPGVRMESTRYPALCSQIQNP
                     EPQLLSPGAKKTCPVTLKVGDYLKAVVEQQGIDVVVRLTGPDGSVVTEVDDSSPKEGP
                     ETLTWVVEKDGTYQLEIETAEGETSSGNYVLKLDPPRPATEPERAQVEIDRLSHEVES
                     LFQQRKYDQAIHLAQEVLDLAERVLGPNHSYTGDCINNLAALYWARGNYDKAEPLFIR
                     ALAIVEEKLGPEHLETAVSFNNLARLYQAKGDLAKAEPLYMRCLAIREKETGPDSLKV
                     SDTLNSLGLLKKEMGNFSEAEAILKRALTIQEKKLGPDHPDVTEVSSNLALLYLEIGK
                     YAKVEPLFLRNLAVQEKVNGPEHPDVATSLNDLAGYYQTRGDYAKAELLFQRSLAIWE
                     KAVGPNHPEVALS"
     gene            complement(1364..>1538)
                     /locus_tag="PKZ53_22885"
     CDS             complement(1364..>1538)
                     /locus_tag="PKZ53_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNJ43350.1"
                     /translation="NVILAVGKSITNRTSKADIGTLLLKYGGGGHANAGTCQVAVDQV
                     EAVLEELFPLLNQ"
BASE COUNT          339 a          411 c          374 g          414 t
ORIGIN      
        1 gctcagtgcg acttctggat ggtttggccc gacagctttt tcccaaatgg ccagtgaccg
       61 ctgaaacaga agttcggctt tcgcatagtc acctctggtt tgataatatc cggccagatc
      121 gttcaagctg gtggccacat ctggatgttc cgggccattt actttctcct gaaccgccag
      181 atttcgcagg aaaaggggtt cgactttggc atatttccca atttcaagat acagcaaggc
      241 cagattagac gacacttcgg taacatcggg gtggtcaggc cctaatttct tctcctggat
      301 tgtcagtgcc cgttttagaa tggcttcagc ctcggaaaaa ttgcccatct ctttcttgag
      361 tagcccaaga ctattgaggg tgtcggaaac tttgagcgag tcgggaccgg tttctttttc
      421 acgaatggca agacatcgca tatagagtgg ttctgctttt gccagatcac ctttggcctg
      481 ataaagccgt gccagattgt tgaagctcac ggctgtttca aggtgctcag gacccaattt
      541 ttcttcaacg attgccaggg cccggataaa cagcggctcc gctttgtcat agtttccgcg
      601 tgcccagtac aaagccgcca gattgttgat acaatccccg gtgtatgaat ggtttggccc
      661 cagaactctt tcggccaggt caaggacctc ctgagccaga tgaatggctt gatcatattt
      721 tcgctgctga aacaacgatt caacctcatg gctgagtcgg tcaatttcaa cctgggcccg
      781 ttcgggttcg gtggccggtc gcggtgggtc aagtttgaga acgtagtttc ccgaactggt
      841 ttcaccttcc gcagtttcga tttcaagctg gtacgtgccg tctttttcca ccacccaggt
      901 cagggtttcc ggcccctctt ttgggcttga atcatcaact tcagtcacca cactgccatc
      961 tggccccgtt agtcgcacca ccacatcaat tccctgttgc tcaaccaccg ccttgaggta
     1021 atccccaact ttgagcgtaa ccggacaggt ttttttggct cctggactga gaagttgagg
     1081 ttccgggttc tggatctggg aacacaaagc tggataccgg gtgctttcca ttctgacacc
     1141 tggcgtttgg aaaactccag gtccaaaaca acacgccaaa acgccgacca caaccacagg
     1201 tctgattgtt ttcatatgtg tgcgaaccag aagatgggcc atcttcagaa agaaagttca
     1261 gagtaacgcc ttcaggcgtg agggtttacg atgggaattg aagccgaacc cctttcgtct
     1321 caagatgata ctctaaacct tgtgcgactg tttttgcgct ttctcactga ttcaacaacg
     1381 ggaagagttc ttccaatact gcctcaacct gatcaacggc cacctgacag gtacccgcat
     1441 tggcatgccc acctccgcca tatttcagca ggagcgtccc gatgtcggct tttgaggtcc
     1501 ggttggtgat tgacttccca accgcgagaa ttacgttt
//
LOCUS       DAOQDO010001816         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92722_length_1537_cov_3.705803, whole genome shotgun sequence.
ACCESSION   DAOQDO010001816 DAOQDO010000000
VERSION     DAOQDO010001816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92722_length_1537_cov_3.705803"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1537)
                     /locus_tag="PKZ53_22890"
     CDS             complement(<1..>1537)
                     /locus_tag="PKZ53_22890"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43351.1"
                     /translation="AHLNTKNGLTNDVVYALAEDPLGRIWVGTYGGGVNCVQDGKVIA
                     TYSVANGLAHNVIRSVFVDRQGVVWIGTDGGGINCIKDGVLTTLSSRDGLSSDIVFSF
                     YEDSADALWIGTNSGLNRLKDGRVTVFTTQQGLFDDKIFQILEDSDGFLWMSCNKGVF
                     QVRRADLEQVGRGEIPLAQSVVYGKADGMRSSQCNGASQPAGWKARNGLLWFPTMAGV
                     TVIDPRHIPINTMPPPVVIEKVLVNNEPLSLYPQRQIELAPGKENFEFYYAGLSLLVP
                     EKVTFQYRLEGFDSDWKSADTRRVAFYTNVPPGKYRFVVRAQNNDGIWSTAPATVEFR
                     LQPHFYQSGWAIFLMTGIVIGGVYGGTQLRLRTLHQRNERLEARIRARTLELAQTIDQ
                     LRVSEKNALELKDKALEAERKALEASATKSMFLANMSHEIRTPMNGVLGMLELLLETR
                     LATDQQEYASIAHHSAESLLQIINDILDFSKVESGKLQLELVAFDLTRTVIESVELFA
                     RQAH"
BASE COUNT          382 a          427 c          370 g          358 t
ORIGIN      
        1 gatgggcctg acgggcaaaa agctcaacac tttcaatcac ggtgcgggtc aaatcaaagg
       61 ccaccagttc cagttgcagt ttgccggatt caaccttcga aaaatccaga atgtcattga
      121 taatctgcaa caatgactcg gctgagtgat gcgcaatcga agcatattct tgctggtcag
      181 tcgccagtcg ggtttcaagc agtaattcca gcatacccag aaccccattc atgggggtcc
      241 ggatttcgtg gctcatattg gccaaaaaca tgcttttggt cgcactcgct tccagggctt
      301 tccgctcagc ctcaagggcc ttgtccttga gttcaagcgc atttttttca gaaactcgaa
      361 gctgatcaat ggtttgtgcc agttcgaggg tccgggctcg aatccgggct tcaagacgtt
      421 catttcgctg gtgcagtgtt cgcaaccgca actgggtacc accatacaca ccgccaatca
      481 caataccggt catcaggaaa attgcccagc ctgactggta aaaatgaggt tgcagcctga
      541 actcaaccgt ggctggcgct gtgctccaga taccgtcgtt attttgagcc cgaacgacaa
      601 accgatactt gcctggcggc acgttagtgt aaaacgcaac ccggcgcgta tcggctgatt
      661 tccagtctga atcaaatccc tcaagccggt actggaacgt gactttctca ggcaccagca
      721 aactgagtcc ggcataataa aactcgaaat tttcttttcc gggggcaagt tcaatttgac
      781 gttggggata gagcgaaagg ggttcgttat tgaccagaac tttctcaatg acaaccggcg
      841 gcggcatagt gttgattgga atatgccggg gatcaatcac tgtgactccg gccatggtgg
      901 gaaaccacaa caggccatta cgcgctttcc agccggctgg ctgtgaggcc ccgttgcact
      961 ggctggagcg catgccatcg gctttgccat acacaactga ctgtgccagg ggaatttcgc
     1021 cacgtccaac ctgctcaagg tcagcccgcc ggacttgaaa cacccctttg ttacaactca
     1081 tccacaaaaa tccatcgctg tcttccaaaa tctggaaaat cttatcgtca aacaacccct
     1141 gttgcgtggt aaagacggtc acgcgaccgt ctttcaaccg attgagacca ctattggttc
     1201 caatccagag cgcatcggct gagtcttcat aaaatgaaaa gacaatatcg cttgataacc
     1261 catcgcgtga cgacagggtc gtaaggacgc catctttgat gcagttgatt ccgccaccat
     1321 cggtgccgat ccaaacaacc ccctggcgat caacaaatac ggaccggatg acattatggg
     1381 cgagaccatt tgccacgcta taggtcgcga tcactttgcc atcctgcacg caattcacac
     1441 caccaccata ggtgcccacc caaattcgcc cgagcgggtc ttcagccagg gcatacacca
     1501 cgtcgttggt gagaccattt ttggtgttta agtgcgc
//
LOCUS       DAOQDO010001817         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92747_length_1537_cov_2.827935, whole genome shotgun sequence.
ACCESSION   DAOQDO010001817 DAOQDO010000000
VERSION     DAOQDO010001817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92747_length_1537_cov_2.827935"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..589
                     /locus_tag="PKZ53_22895"
     CDS             38..589
                     /locus_tag="PKZ53_22895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ43352.1"
                     /translation="MCAGCEPEIALVGKPAPDFTLEGVFGNSFKKVSLSEYRGKWLVL
                     FFYPLDFTFVCPTEVTGFSDRVSEFHDLGAEVVAVSTDSVYSHRAWIETSREKNGVGP
                     INFPLASDITKQVSRDFGVLIEDRGIALRGLFIIDPNGVLQYQVVHSLNIGRNVDEAL
                     RVLEALQSGERCPINWKRGQSHI"
     gene            792..1067
                     /gene="purS"
                     /locus_tag="PKZ53_22900"
     CDS             792..1067
                     /gene="purS"
                     /locus_tag="PKZ53_22900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNJ43353.1"
                     /translation="MKATVHVTLKPGVLDPQGQAIHHAAETLGYQAIAAIRQGKFFEI
                     SIADGVSPETAQAEVEKLAHEVLSNPVIEDFTVSIGPLQPAIPGGQA"
     gene            1064..>1537
                     /gene="purQ"
                     /locus_tag="PKZ53_22905"
     CDS             1064..>1537
                     /gene="purQ"
                     /locus_tag="PKZ53_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNJ43354.1"
                     /translation="MKFGVLVFPGSNCDHDTYHVISKVIGQPVQFIWHRNTTLNGCDV
                     VFLPGGFSYGDYLRCGAIASFSPIMQAVKEFAAQGGYVVGICNGFQILCESKLLPGTL
                     LRNRDLKFTCQFTHLRVENNLTPFTRNCLPSSVLSLPIAHGEGNYFCTAEELSDMQ"
BASE COUNT          354 a          422 c          369 g          392 t
ORIGIN      
        1 attaacccca attcttatgt catgttgctc ctctggaatg tgcgccggct gcgaacctga
       61 aatcgcactc gtgggcaaac ccgcccctga ttttacgctc gaaggtgtct tcggaaactc
      121 cttcaaaaaa gtatctcttt cggaatatcg tggaaaatgg ctggtcctct ttttctaccc
      181 acttgacttc acctttgtct gtcccactga agtaactgga tttagtgatc gagtcagtga
      241 attccatgat cttggcgccg aagtggtggc ggtcagcacg gacagcgtgt actcacatcg
      301 ggcctggatc gaaacctcac gtgagaaaaa tggggtcggg ccgatcaact tcccactcgc
      361 cagcgacatt accaaacagg tttcccgcga ttttggggtc ctgatcgaag accgcggcat
      421 tgccctgcgt gggttgttca ttattgatcc caatggcgtc ctccagtatc aggtcgtcca
      481 ctcactcaac attggtcgca acgttgacga agccctccgg gtcctggaag ccttacaatc
      541 cggggaacgc tgcccaatca actggaaacg cggtcaatca catatctagc agggctaagg
      601 gcttggggct tgaagacatc gggctgggct gcggatacct gtttgcttgt gccccgagcc
      661 ctcatctgtg gtatactggc cagtttttgc ccgacgcctg ggtaaaaacc tgagtattgc
      721 accgggctga agttcgagcg ctccagttca ccaccctcaa ccatcattcc cgactctcaa
      781 tctgatttca tatgaaagct actgttcacg taaccctcaa acctggagtt cttgaccccc
      841 aaggacaggc cattcaccac gcggctgaaa cactcggcta ccaggcaatt gccgccattc
      901 gccagggaaa attttttgaa atctcaatcg cagatggcgt ttcgcctgaa accgcgcagg
      961 ctgaagttga aaaactggcc catgaggttc tctccaatcc agtcattgaa gacttcacgg
     1021 tctcaatcgg accacttcag ccagccattc caggaggcca ggcatgaaat tcggggtact
     1081 tgtttttcct gggtcaaact gcgaccacga cacctatcac gtcatcagta aagtcattgg
     1141 ccagccggtt caatttatct ggcatcggaa tacaaccctg aacggatgcg atgtcgtgtt
     1201 cctgccgggc gggttttcct atggggatta tctgcgatgt ggcgctatcg ccagcttttc
     1261 accaattatg caggcggtga aagaatttgc cgcccagggc ggctatgttg ttggcatttg
     1321 caatggattt caaattttat gtgaatccaa attacttccc ggaacgttgc tccgcaaccg
     1381 tgacctgaag ttcacctgcc agtttacaca cctgcgcgtt gaaaataatt taaccccctt
     1441 tacacgcaat tgtttgccat cgagtgtcct gtcactgcca attgcccatg gcgagggaaa
     1501 ttatttctgt acggcggaag aattatcaga catgcag
//
LOCUS       DAOQDO010001818         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92963_length_1535_cov_3.249324, whole genome shotgun sequence.
ACCESSION   DAOQDO010001818 DAOQDO010000000
VERSION     DAOQDO010001818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92963_length_1535_cov_3.249324"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..842)
                     /locus_tag="PKZ53_22910"
     CDS             complement(186..842)
                     /locus_tag="PKZ53_22910"
                     /inference="COORDINATES: protein motif:HMM:NF020544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HNJ43355.1"
                     /translation="MGIECYLRHITPHWLEQLTSDPTLGLQFVTAITPFVAEDQWKSM
                     GIDTNDPQQIMDHLSRSGPVSAPLKHHLYEKQAVKAYLKQTFRDELLRGKLLDTHNAW
                     DAIHAILSGTTELTGTTLDKVIKSGTKIGNDDNFNYGAPHFLTPAEVKAVYDAISPIT
                     KTEFRRRFDALSADEAELVYGFDQDETGFQHIWNVFRKVADHYREAAEEGLAMLVFFA
                     "
     gene            1086..1394
                     /locus_tag="PKZ53_22915"
     CDS             1086..1394
                     /locus_tag="PKZ53_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43356.1"
                     /translation="MTFEEMERTMEFIVNQQAKFSVDIDLLKEAQAILTANMAEMKVQ
                     AEQDRELMKAMFRHLSDNAEADRRMVREMVQDLKGLFYQVDAKADKALRLAQKNDTKE
                     "
BASE COUNT          426 a          317 c          386 g          406 t
ORIGIN      
        1 gtcagtagcc cgtagtcagt agttcactaa gtttatttga ttgaatcact tgactatttt
       61 ctaatgcgaa gatttcattc caaattggta taacacaacg cggtgccgtt ttaaggactt
      121 cactcgaaga gggtcaggat ggcggatgac tgtttggttg tccgtcatcc tttgtttttt
      181 ggaaatcagg caaaaaagac cagcatggcc agtccttcct cagccgcttc gcggtaatga
      241 tccgccactt ttcgaaagac attccagatg tgttggaatc cggtctcatc ttgatcaaac
      301 ccatagacca gttcggcctc atcagcagag agcgcatcaa accgtcttcg gaactcagtt
      361 ttggtgatgg gactgatggc gtcgtacacg gctttgactt cagccggtgt taagaaatgc
      421 ggcgcgccgt agttaaaatt gtcatcattt ccgattttcg tgcccgattt aatgacctta
      481 tccagagtcg ttcccgtgag ttcggtcgtg cccgacaaaa tcgcgtgaat cgcatcccag
      541 gcattgtggg tgtcgagcag tttcccccgc agcaactcat ctctaaatgt ttgttttaaa
      601 taggctttga ccgcctgttt ttcatacagg tggtgtttga gcggtgctga aaccgggccg
      661 gatcttgaga gatgatccat aatctgttgg ggatcgttgg tgtcaatccc cattgatttc
      721 cattgatctt cagccacaaa cggtgtgatg gctgttacaa attgaagccc cagcgtgggg
      781 tcagatgtca gttgttccaa ccagtgcggc gtgatatgcc gcaggtaaca ctcaattccc
      841 attcgatttt tccagaaata ttgaataatg agtcgtgaag ggcagggcga ataggattag
      901 ctgaaaaaga ccagcatggc aagcccattt tcagtagtat gatcaagaac aatcattgag
      961 tgggagtcaa tcaggcacag ccagtgtcgt ggcccgtgga ttgccttgaa aatgggcaac
     1021 cagtattgtt tgtgaaaaac cagtttcgac aatagcctga actgaagatt taaggagaag
     1081 tcaaaatgac ctttgaagag atggaacgca cgatggaatt tattgtcaat caacaggcta
     1141 aattcagcgt tgacattgac ttgctgaaag aggcacaggc tatcttaacg gctaatatgg
     1201 ctgaaatgaa ggtccaggcg gaacaggatc gggaattgat gaaagcgatg tttcgacacc
     1261 tttcggacaa tgctgaagct gaccggcgga tggttcgtga aatggtccag gatttaaaag
     1321 gtctgtttta tcaggttgat gcgaaagccg ataaagctct gagactggca caaaagaatg
     1381 ataccaaaga ataaagtgac ctaaactgag cgctcaaaga tcaaaatgga gaaatcaaaa
     1441 tgacctttga agagatggaa cgcacgatgg aatttattgt caatcaacag gctaaattca
     1501 gcgttgacat tgacttgctg aaagaggcac aggct
//
LOCUS       DAOQDO010001819         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_92984_length_1535_cov_2.673649, whole genome shotgun sequence.
ACCESSION   DAOQDO010001819 DAOQDO010000000
VERSION     DAOQDO010001819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92984_length_1535_cov_2.673649"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..849)
                     /locus_tag="PKZ53_22920"
     CDS             complement(121..849)
                     /locus_tag="PKZ53_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ43357.1"
                     /translation="MSAIFATDNLVDIVYPESDGKPMAENTLQYEWIVMIKGGLDAVL
                     PNDFVAGDLLWYPVEGKPKIAQAPDVMVAIGRPKGHRRSYLQWQEEHVAPQVVFEILS
                     PGNTASEMTKKELFYNEHGVEEYYLYDPEKNDLFGWIRSEKGLTAIPAIDTWTSPRLG
                     IRFERTPETLIIFRPDGRRFETFAELEARAEAEHQRAEAEAQRAEAEAQRAQQALSQI
                     EQERLITERLKQKLLELGIDPETV"
     gene            complement(1138..>1535)
                     /locus_tag="PKZ53_22925"
     CDS             complement(1138..>1535)
                     /locus_tag="PKZ53_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNJ43358.1"
                     /translation="ADAGNQDLTPIFEQALTAGAWNEGTVGYFKKAIPQFQQALGDKQ
                     REYAIKDAMIAATHLVLAAESLGLSTCYMNGWIEDKVKAAIGASDNPNLAIAVVIPVG
                     YAAEPRKDPGRFPWNVNVFADTLETPFQK"
BASE COUNT          357 a          392 c          391 g          395 t
ORIGIN      
        1 ggtaggttcg aattgaaatc atgtgagggt gaaatgactg agtcggtgga aacaaagggg
       61 cgcggtaact gactggccgc gccctgatgt tgagtagata tcctgatgtg cggtatcggt
      121 ttaaaccgtt tcaggatcaa ttccaagttc gagcagtttc tgcttgagtc gttcagtaat
      181 cagtcgctcc tgttcaatct gggagagtgc ctgttgagcc cgttgcgctt cagcttcagc
      241 ccgttgcgct tcagcttcag cccgttgatg ctcagcttca gcgcgtgctt caagctcggc
      301 aaaagtctca aaccgccgtc cgtctggtcg gaaaataatg agggtctcag gtgttcgttc
      361 aaatcggata cccagtcgag gactggtcca ggtgtcaatt gctggaatgg ccgtcaagcc
      421 cttttcagag cgaatccacc cgaaaaggtc atttttctca gggtcataga ggtaatactc
      481 ttcaacccca tgttcattat aaaaaagctc ttttttggtc atttccgagg cggtgttccc
      541 tggtgaaaga atctcaaaca caacctgcgg tgcgacatgt tcttcctgcc attgcagata
      601 cgagcggcga tggcctttgg ggcgaccaat ggccaccatc acatcgggcg cctgggcgat
      661 ctttggtttt ccttcgaccg gataccacaa caggtcccca gccacaaaat cattggggag
      721 caccgcatct aacccgcctt tgatcatcac aatccactca tactgcagtg tgttttcagc
      781 cattggtttt ccatcgcttt ctggataaac aatgtcaacc agattatcgg ttgcaaaaat
      841 tgctgacata gcatcctcct tggggtttgt actgtgggtt atgattggcg tggctcgaag
      901 aaaacacccc ccaactataa gctgaaaagg tagcttgtgc aatttttcag tcagggttcc
      961 atacaccata aaaaaacccc accgtggcag gtggggtttt cgcaaaaaac acagcacaac
     1021 tgatagaaaa agatttcggt tcagcgtaac gccttcaggc gagaggtttg acgagtggga
     1081 cgccagatga aaccctcttg ttccaagacg atactctgaa cctttctctg aagaaggcta
     1141 tttctgaaat ggcgtctcca gcgtgtcggc aaagacattg acgttccagg gaaagcgtcc
     1201 tggatctttg cgaggttcag cggcataccc gaccggaatc acgacggcaa tcgccagatt
     1261 gggattgtct gaggcgccaa tcgccgcttt gactttgtct tcaatccagc cgttcatata
     1321 acaggttgac agccccaggc tttctgccgc caggaccaga tgggtcgccg caatcatggc
     1381 gtctttgatg gcgtattcac gctgtttgtc gcccagtgcc tgctgaaatt ggggaattgc
     1441 cttcttgaaa tatccgactg tcccttcatt ccaggcacca gcggtcagtg cctgctcgaa
     1501 aatcggggtc agatcctggt tgccagcgtc agcgt
//
LOCUS       DAOQDO010001820         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93001_length_1535_cov_2.362838, whole genome shotgun sequence.
ACCESSION   DAOQDO010001820 DAOQDO010000000
VERSION     DAOQDO010001820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93001_length_1535_cov_2.362838"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKZ53_22930"
     CDS             complement(<1..594)
                     /locus_tag="PKZ53_22930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ43359.1"
                     /translation="MAEQILANHQQPAEPKSIRVAIIEDLRDLREGLQLFLENSTGFE
                     LTGVFGSMEEALRGLPTSIPDAILVDLGLPGMSGIEGIRVMKERFPDVQLIALTVFDE
                     DDKIFDALCAGANGYLLKKTPPAKLLEALRDVTSGGAPISPEIASRVIYLFRQFRPPA
                     DREACHLTQQETQLLKLFVDGHNYKTAAIELGVSVNTI"
     gene            complement(534..>1535)
                     /locus_tag="PKZ53_22935"
     CDS             complement(534..>1535)
                     /locus_tag="PKZ53_22935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNJ43360.1"
                     /translation="SGLSFAVGDDGLFQYRLEGTDDDWSPPTRQRSVTLANLSPGDYR
                     FEVRAVSASNRLSSQPAVIEFVIKPPIWQRWWFLLLLASIPALVAYGVHSYRLAQVLE
                     RERLRMRIAADLHDEVGAHLSQVAILSEVIRQKAGGMGEQVDDLLSRMARNSIEAVNT
                     MSDIVWAINPQKDRRKDLVRRMRQLAGELLSASDIRFRLEAAESGADKKMPPDTRRQI
                     YLIFKESLNNIVRHASCTEVQIEFRVQASQIVLQITDNGKGFEPRLARDGNGLTNMRR
                     RAESLGGELVIETRPNQGTTVRLTAQLLPKNEPGKVRKWLSKYLPITSNRLNPNQSE"
BASE COUNT          358 a          422 c          399 g          356 t
ORIGIN      
        1 aatggtattg acgctgacgc cgagttcgat ggcggcggtt ttgtaattgt ggccatccac
       61 aaagagcttt aataactggg tttcctgctg ggtgagatgg cacgcttcgc ggtcggcagg
      121 tggccgaaat tgccggaata aataaatcac tcgcgaggca atttccggtg agatcggggc
      181 gccaccggaa gtgacatccc ggagcgcttc gagtaatttg gccggtggcg ttttcttcag
      241 aagatacccg ttggcaccgg cacagagcgc atcaaagatt ttgtcgtctt cgtcaaacac
      301 ggtcagggca atcaactgga cgtccgggaa ccgctctttc atcacgcgaa tgccttcgat
      361 tccagacatt ccaggcaacc ccagatccac cagaattgca tccggaatac tggtcggcaa
      421 gccacggagc gcctcttcca tcgaaccaaa caccccggtc aattcaaacc cggttgagtt
      481 ttccagaaag agttgaagcc cttcgcgcag gtcacgcaaa tcttcgatta tggctactcg
      541 gattgatttg ggttcagccg gttgctggtg attggcaagt atttgctcag ccattttcgt
      601 acctttccag gttcattttt gggtaaaagt tgggccgtca ggcgaaccgt ggtgccctgg
      661 tttggacggg tctcgataac cagttcccca cccagacttt cagcccgccg acgcatattg
      721 gttaatccgt ttccgtcacg agccagtcgt ggctcaaagc ctttgccgtt gtcagtgatc
      781 tgcaacacaa tctgactggc ctgcacccgg aattcaatct gcacttcagt gcaactggcg
      841 tgacggacaa tattattcag actttcctta aaaattagat aaatctggcg tcgcgtatcg
      901 ggtggcattt ttttgtcagc gcctgattcg gcggcttcca atcgaaaccg aatgtcagac
      961 gccgaaagca gttcgccagc cagttggcgc atccggcgga ccagatcctt gcggcggtct
     1021 ttttggggat tgattgccca tacaatatcg ctcatggtat tgacggcttc gattgaattc
     1081 cgggccatcc gcgacagcaa gtcatccacc tgttcgccca tcccacccgc tttttgccgg
     1141 atcacctcac ttaaaatagc cacctgtgac agatgggcgc caacttcatc atgcagatca
     1201 gcggcgatgc gcatccgcaa tcgttcgcgt tcgagcacct gagctaatcg gtatgaatga
     1261 acaccatagg caaccagagc tggaatagag gccaggagca agagaaacca ccagcgttgc
     1321 cagatgggcg gcttaattac aaactcaatg actgcgggtt gggaactgag gcgattgctg
     1381 gcactcactg cccgaacttc aaagcgatag tcacctggtg agaggttggc cagagtaacc
     1441 gaccgctgac gggtcggagg gctccaatca tcgtcagtcc cttcaagccg atactggaac
     1501 aacccatcat cgccaaccgc aaaggacaat cccga
//
LOCUS       DAOQDO010001821         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93006_length_1535_cov_2.247297, whole genome shotgun sequence.
ACCESSION   DAOQDO010001821 DAOQDO010000000
VERSION     DAOQDO010001821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93006_length_1535_cov_2.247297"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..>1535)
                     /locus_tag="PKZ53_22940"
     CDS             complement(60..>1535)
                     /locus_tag="PKZ53_22940"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43361.1"
                     /translation="GEEQATLQKEIDGLKTEYREIQNQIRTQSPHYAALTQPQPLTAQ
                     EIQTQVVDSDTALLEYALCSTTSYLWVVTPTGVKTFTLPPRDVIEKKARQVRELLLER
                     TKTEPAPASTQNSKSEASRNLQKVTDQRKVRIRQADEQFFQVAAQLSQMILGPAAQEL
                     GTKRLVIIPDGALQYIPFSTLCLPGPTFNQTRPGTRYTEPLIVHHEIVTLPSASTLAV
                     LRRETANRSPAQNQLAILADPVFGGTDDDRVHAIEPAANPQPKQNEDQPESATDSASQ
                     YVATRLLGRSEDAPASESKNSLQIPRLFFTRNEANQVLNLAGETQNLKVLDFSVNRQL
                     ISEGRLSSYRYIHFATHGWLDAEHPEFSALLLSMVNEKGEPQDGFLRALDIYNLQLPA
                     EMVVLSACQTGLGKEIKGEGVVGLTRGFMYAGAKRVVVSLWSVSDKATADLMTRFYQK
                     IFREGMSPAAALRAAQLELWQSEKWHAPFFWAPFVIQGEWK"
BASE COUNT          334 a          394 c          430 g          377 t
ORIGIN      
        1 cggaacccgg aacccggaac ccggaacccg gaacccggaa cccggaaccc ggcttttcct
       61 cacttccact cgccctgaat cacaaacggt gcccagaaaa acggcgcatg ccatttttca
      121 ctctgccaga gttcgagttg ggcggctcgc agggcagcgg ctggcgacat tccttcccgg
      181 aaaatcttct ggtaaaagcg tgtcatcaga tcagcagtgg ctttgtcgct cacgctccag
      241 agactgacca caacacgttt ggcaccggca tacataaatc cacgtgtcag cccaaccaca
      301 ccttcaccct tgatttcttt ccccagacca gtttgacagg ctgacaaaac caccatttca
      361 gccggaagct ggagattgta aatatcgagt gcccgcaaaa aaccgtcctg aggctcgcct
      421 ttttcattca ccattgagag cagcagggcg gaaaactcag ggtgttcggc gtcaagccag
      481 ccgtgggtgg caaagtgaat atagcgataa gaactcaacc gaccttctga aatcagttgg
      541 cgattgaccg agaagtcaag cactttgaga ttttgggttt cgcctgccag gttcagaacc
      601 tggttggctt cgttacgggt gaaaaacagg cgtggaatct ggaggctatt ctttgactct
      661 gaggccggcg cgtcttcact gcgtccaagc aatcgggtgg caacatactg gctggcgctg
      721 tcagtggctg attcaggctg atcttcattt tgttttggtt gtgggttggc agcaggttca
      781 atcgcatgta cccggtcatc atccgtgccg ccaaaaactg gatcggccag gatcgcaagc
      841 tggttttgcg caggtgaccg attcgccgtt tcacgccgca acacggccag agtcgaggct
      901 gaaggcaggg tcacaatttc gtggtgaaca atcagtggtt cggtatatcg ggttccgggt
      961 ctggtttggt tgaaagttgg gccgggaaga caaagagtac taaatggaat gtattgcagg
     1021 gctccatcag gaatgatcac cagccgtttg gttccgagtt cctgggctgc gggaccgagg
     1081 atcatctgac tgagctgggc agccacctga aaaaactgct cgtcagcctg gcggatacgc
     1141 acttttcgtt gatccgtaac cttctgcaga ttccgagaag cctctgactt tgagttctga
     1201 gtggaggctg gagctggctc tgttttggtc cgttcaagga gcaattcacg cacctgccgg
     1261 gccttttttt caatcacatc acgtggcggc aaggtaaagg ttttgacacc tgtcggggtg
     1321 acaacccaca ggtaactggt tgtgctacac agggcatatt caagcaatgc ggtgtcgctg
     1381 tctacgacct gggtttgaat ttcctgagcc gtgagtggtt gaggctgggt aagggcagcg
     1441 tagtgcgggc tttgggtacg aatctggttt tgaatttccc ggtattcggt ttttaacccg
     1501 tcaatttctt tttggagcgt ggcctgttcc tctcc
//
LOCUS       DAOQDO010001822         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93088_length_1534_cov_2.737661, whole genome shotgun sequence.
ACCESSION   DAOQDO010001822 DAOQDO010000000
VERSION     DAOQDO010001822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93088_length_1534_cov_2.737661"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1268
                     /locus_tag="PKZ53_22945"
     CDS             360..1268
                     /locus_tag="PKZ53_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43362.1"
                     /translation="MPLPFPDPEEEAPLSLEAPIPAEIPAVATPAESSRLNYFNYYTE
                     VEEFFVARRGSPMMISPLDWSLVESWKEAGIPLAVILRGITRAFEKRAREVESSSIRK
                     TFKKVNTLFYCQQSVMEEYATHLETHVGESPSGDASTQEETELSEAQAAENDLFPPAT
                     VRRYLEEVSAELEHTRQVVQFQATEMYPHTRWVEAIDRAMAHLAELMGELPKPGEPRR
                     TPINFEKMEQDLTWIEELLYTALCADAPPEVLTEATNEAVSQLKPHKKRMDAAVYQQT
                     LENYRARQLRTRYQVPRLSLFYLRPE"
BASE COUNT          370 a          404 c          375 g          385 t
ORIGIN      
        1 cagaacacaa accagggttc aaggccaccg tgttctgcct gttggcgtgc ggcttcagcc
       61 gtgtcgccga ctccacaaag ggtgccatca gttaatgcaa cccactgacc agcataggtt
      121 tcccggtttt gatccagcca ggcttttttt cgggtagctt catcaatttc gcgtagcata
      181 agtttttgtc caggttttcg aagagggcac agttcaaagc aacggtgccc ttccatcatt
      241 ttgtcagcaa gacggtctgg agatttttta tggattttgg cggaaaggtt gtgatttttt
      301 tcatcggcct ttaccttttc ataatcacat aatccccttt ttcttgaaag ctgaccgtca
      361 tgccattgcc atttccagac cctgaagaag aagcccccct ctctctcgaa gcgccgattc
      421 cagccgaaat ccctgcggtt gcgacgccgg ctgagtcgtc acgattgaat tattttaatt
      481 actacactga agtcgaggaa ttttttgttg cccggcgcgg atcgcctatg atgatttcgc
      541 cgcttgactg gtcgctggtc gaaagctgga aagaggcggg cattccgctg gctgtgattt
      601 tgcgtggcat tacccgtgcc tttgagaagc gggcccgcga ggtagaatcg tcctcgattc
      661 gcaaaacatt caagaaggtc aacacgctgt tttattgtca gcaatcggtg atggaagaat
      721 acgccaccca tctggaaacc cacgtcgggg aatcgccgtc aggtgatgcc tcgactcagg
      781 aagaaaccga actctctgaa gcccaggcgg ctgaaaacga tctgtttcca ccagcgacgg
      841 tgcggcgcta cctcgaagaa gtctccgctg agcttgaaca cacccggcag gtggtccaat
      901 ttcaggcgac cgaaatgtac ccccataccc ggtgggtcga agccattgac cgggcaatgg
      961 cccatctggc tgagttgatg ggtgagcttc cgaaaccggg tgaaccgcgc cggacgccaa
     1021 tcaattttga gaaaatggaa caggatttga cctggattga agaactgctc tacaccgcgc
     1081 tctgtgccga tgccccgccc gaagtcttga cggaagccac caacgaagcc gtgtcacaac
     1141 tcaaaccgca caaaaaacga atggacgccg ccgtttacca gcaaacgctc gaaaactacc
     1201 gcgcccgtca gttacgaacc cgctatcagg ttcctcggtt gagtttgttt tatttgcgac
     1261 cggaataaat ccaacccgat ttctaaagct caagccacgg aatacattga atccattgga
     1321 gaagaacagt tcttcttctc cctgattcag tctgttccgt ggcttctttt ttggtgctgg
     1381 gaaatacaag gccgggcgac ctcaaaactc attccccgga gaaatagttt tcctgactca
     1441 atttttttgt ggacttttaa aaagaagtag tgtactactg tactagtgta ccgctagtgc
     1501 gatacactag tacagtgatg aaaacatttt gaac
//
LOCUS       DAOQDO010001823         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93209_length_1533_cov_2.783491, whole genome shotgun sequence.
ACCESSION   DAOQDO010001823 DAOQDO010000000
VERSION     DAOQDO010001823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93209_length_1533_cov_2.783491"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1061)
                     /locus_tag="PKZ53_22950"
     CDS             complement(201..1061)
                     /locus_tag="PKZ53_22950"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ43363.1"
                     /translation="MKPVTLSAQPGATAQPKSFYGMTGESWRLTGITFTESSYPAGLA
                     MTRHDHCHPYCSFVLAGAYAETVGSMTRSVEPNHITFHPREEAHAVRFGPHPSRIFRV
                     EFNANWLADLVGNRQTLHQPMHLRNEPFKRHRVALYREFSRQDRWSALAVEGIVLELI
                     AELGRLFEAPSSTRRQPSWIQPIREQLEAEFLTPPSLAQLAQSVNVHPVTVAKTFRTI
                     FHVTMSEFIRQRRIEFACDQLLQSQLPITQIALESGFYDQSHFTNAFKRVLGVTPAEY
                     RTMNQKPVKL"
     gene            1107..1292
                     /locus_tag="PKZ53_22955"
     CDS             1107..1292
                     /locus_tag="PKZ53_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43364.1"
                     /translation="MERGLDVMVSDQYGASRIGVAAMVLDQTGRGPHRSFANVVCGVE
                     SLVVRSCSLRLGLFSET"
     gene            complement(1354..>1533)
                     /locus_tag="PKZ53_22960"
     CDS             complement(1354..>1533)
                     /locus_tag="PKZ53_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43365.1"
                     /translation="LERRPGQRSTRQRKQIKTKVAKVEAIGICSNRLKRPSRDEKRKN
                     DLLHHVTQKNTPESR"
BASE COUNT          328 a          327 c          462 g          416 t
ORIGIN      
        1 ggtcagatgc cggcaggatg ccggcgctcc caggaattct ttatcttaga caattgattg
       61 tcttggaaaa aattagggtg gttggaaaag tttgacttca gttctggcgg tgaaagattc
      121 gaaactgggc aatttgagat acagccaggt gttgtaggaa caatggcaag caatccgaca
      181 ctgagaaaaa gcgggaataa ttacaatttt acaggtttct gattcattgt acgatattca
      241 gccggcgtta cccccaggac tcgtttaaag gcattggtaa aatggctctg atcataaaac
      301 cctgattcaa gggcaatttg agtaattggc aactggcttt gtaatagttg atcacaggca
      361 aattcgatac gtcgctggcg gatgaactcg ctcattgtaa cgtgaaaaat tgtgcgaaag
      421 gttttggcta cggtgactgg atggacattg acggattggg ccagttgggc cagcgatgga
      481 ggtgtcagaa actcagcctc cagttgctcg cggattggct gaatccagga tggctggcgg
      541 cgtgtgctgg atggcgcttc aaacagccgg ccaagctcgg cgatgagttc gagcacaatc
      601 ccttccactg ccagggccga ccatcggtcc tgacgggaaa attcacggta caaggcaacc
      661 cggtgacgtt tgaatggttc gtttcgcaga tgcatcggct ggtgcagtgt ctggcggttt
      721 cccaccagat cagccagcca gttggcattg aattccaccc ggaaaatccg ggacggatgg
      781 ggtccaaacc gaacggcgtg ggcttcttcg cgaggatgaa acgtgatgtg gttcggttca
      841 accgaacgag tcatcgaacc aaccgtttca gcatacgcac cggccagaac aaagctgcag
      901 taggggtgac agtggtcgtg gcgggtcatt gcaagccccg ccgggtaact cgattcggtg
      961 aaagtaattc cggtcagtcg ccagctttca ccggtcattc cgtaaaacga tttcggttgt
     1021 gcggttgccc ctggctgagc agagagggtg actggtttca ttgcgaaacc ctcctgaggt
     1081 gacgtcggtc tggattgaag ttttgggtgg aacgtggttt ggatgtgatg gtgtcggacc
     1141 agtacggagc aagtcggatc ggtgttgcag cgatggtgct ggaccagact ggccgcggcc
     1201 cgcaccgatc ttttgcgaat gtggtttgtg gagtggaatc gctggttgtt cggtcgtgtt
     1261 ccctccgatt gggtttgttt tcggaaacgt gatgttttgt ttcgggaatg tttctgacca
     1321 cagggtgact tgagagtatg cgtttaattg cgtttatcgg ctctctggtg tgtttttctg
     1381 ggtgacatga tgtagtaagt cgttttttcg tttttcgtcc cttgacgggc gttttaggcg
     1441 atttgagcaa attccgatgg cttccacttt cgctactttg gttttgattt gttttcgttg
     1501 gcgagtactg cgttgaccag gtcgtcgttc cag
//
LOCUS       DAOQDO010001824         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93371_length_1532_cov_1.548409, whole genome shotgun sequence.
ACCESSION   DAOQDO010001824 DAOQDO010000000
VERSION     DAOQDO010001824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93371_length_1532_cov_1.548409"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..991
                     /locus_tag="PKZ53_22965"
     CDS             164..991
                     /locus_tag="PKZ53_22965"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ43366.1"
                     /translation="MGRQAEIALYPERLTEDELAWLVESKPGSRFVSLLSVSRINKEK
                     TPQARIDSIELLGKLKRADLPDVTEVLCQSLKDESPIVRAAAVQALGQLGSNTDDVVT
                     ALKNATADSDEDVKTMAWHTLSQLGITPLESVEIPPLYWIETPPAESFRPVLVSHSAT
                     DPFGPTPVAKRYQKRRDFSDQEGGPRVGFVSVEEIGNWRLIFKAVARNQFKGLEIQAI
                     LYREGGTDEERLECVLKLEATEDPTWVGCELIVDEPEWFLDAKRDGKIGFEPALNQK"
     gene            complement(1022..1288)
                     /locus_tag="PKZ53_22970"
     CDS             complement(1022..1288)
                     /locus_tag="PKZ53_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43367.1"
                     /translation="MSIAKRPQNKPANVDQKEIHEFINKGGTPPQSQPVEKEVTSLQL
                     RIPSDLLARVDSLVKSRNVKTPRHYWLLEAIVEKVEREEQKNQN"
BASE COUNT          387 a          330 c          389 g          426 t
ORIGIN      
        1 actgaacctt tccgattgga tttcaagttg aggatttatg gctactgaag atttacttaa
       61 tcccgcaaaa tattctgtta ttgaacggat tttagaaaac cacgccagga gaccaggggc
      121 acagcctaaa gcgactgcat caccaacttt tcctcaagtt tcaatgggtc gccaggcgga
      181 aattgcctta taccctgagc gactcactga agatgaactt gcgtggctgg ttgagtcaaa
      241 accagggagt cgttttgtaa gcttgctgtc cgtttcacgg atcaacaaag aaaagacgcc
      301 ccaggcacga atagattcta tcgaactgtt agggaagctg aaacgagctg atcttccgga
      361 tgtaaccgag gtactgtgtc aatcgctcaa agatgagtct ccaatagttc gagccgccgc
      421 agttcaagca ttggggcaac tcggaagcaa tacggatgat gttgtgacgg ctttgaagaa
      481 cgccacggct gattcagacg aagatgtcaa aaccatggcc tggcacaccc ttagccagct
      541 tgggatcaca ccactggagt cggtggaaat ccctccgctt tactggatag agactccacc
      601 ggcggagtca ttccggcctg ttttggtatc acactcagcg accgatccct ttggtccaac
      661 gccagttgca aaacgatatc aaaagcgtcg ggacttctct gaccaggagg gtggtcccag
      721 ggtcggtttt gtttcggtcg aagaaatcgg aaactggagg ttgatattca aagcagttgc
      781 caggaaccag ttcaaaggcc ttgaaattca agcaattctg tatcgagagg gaggtacaga
      841 tgaggaacgg ttggaatgcg ttttgaaatt agaggcgact gaagatccaa cctgggttgg
      901 ctgtgagttg attgttgacg aaccagaatg gtttcttgat gccaaacgtg acggtaaaat
      961 tgggtttgaa cctgcgttga atcagaaatg attgattaca atatttttgg ttcagaaggt
     1021 gtcagttttg gtttttttgt tcctcgcgct cgactttttc aacgattgct tccagaagcc
     1081 agtaatggcg tggggttttg acattccgac ttttcaccaa cgaatcaact cgggccagga
     1141 ggtctgacgg aattctgagt tgcaaagacg tgacttcttt ctcaactggc tgcgattgcg
     1201 gcggagtccc tcccttatta ataaactcgt ggatttcttt ctgatcaaca ttcgctggct
     1261 tgttttgtgg gcgcttggca atgctcattg ggttctcctg cttctaaagt ggtattgatg
     1321 aaatatgata gttgtatcta ttcagtatct atctgatatc tatttaatgt ctgattagca
     1381 tctaacttat cccaaacaca tactgataga gcgccttgac ttcattgatt gctttttcgt
     1441 cgtggggttt gagttcagtg accgccagtc cctgagaggc agcatttcca aatgccttgc
     1501 gcgtaccaag agaggttggg attagtgaca gg
//
LOCUS       DAOQDO010001825         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93411_length_1531_cov_2.956640, whole genome shotgun sequence.
ACCESSION   DAOQDO010001825 DAOQDO010000000
VERSION     DAOQDO010001825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93411_length_1531_cov_2.956640"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1049)
                     /locus_tag="PKZ53_22975"
     CDS             complement(63..1049)
                     /locus_tag="PKZ53_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ43368.1"
                     /translation="MRAFVTGGTGFIGGNLIPELLASGSEVRALVRPGSDRKNLEGLA
                     IDCIEGDLNDEHFLHRALDGCQLVFHVAAHYSLLRRDSAAIYRSNVDGTKNLLRAARK
                     TNIRRFVHTSSVAAIGVPPQGQEADETTRTTVDELVSAYKKSKFLAEEAAVAAAREGL
                     PVVIVNPSTPIGPRDVKPTPTGNIVLRFLKREMPMYVHTGLNLVDVRDVVKGHLLAAE
                     KGRVGERYILGNRNVTLKEMVDMLEVITGLPAPTKAIPHWIPIAVGYIDELFISRLTG
                     RTPTVEVDSAKMARHAMYYRSDKAIRELGLPQTPVETALRDAVEWFRANGYV"
     gene            1151..>1531
                     /locus_tag="PKZ53_22980"
     CDS             1151..>1531
                     /locus_tag="PKZ53_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNJ43369.1"
                     /translation="MKKLFAAENIMNLLLVFVPVAMILEWVIHAQPIWIFVVSCLAII
                     PLAGLMGKATEHLSEKLGEGIGGLLNATFGNAAELIIAFMALREGLYDVVKASITGSI
                     IGNLLLVFGLSALVGGLKFPNQTFN"
BASE COUNT          346 a          406 c          390 g          389 t
ORIGIN      
        1 acccggaacc cggaacccgg aacccggaac ccggaacccg gaacccggaa cccgtgtctt
       61 tctcacacat atccattggc gcgaaaccac tccacagcat cgcggagtgc agtttcaact
      121 ggggtttgag gcaggccaag ttcgcggatg gctttatcgc tccgatagta catggcgtgt
      181 ctggccattt tggcactgtc aacttcaacc gttggggtcc ggccagtgag ccgactgatg
      241 aacaattcgt caatgtaacc cacggcgatt ggaatccagt gtggaatagc tttggtcggt
      301 gcgggcagac ccgtgatgac ttcgagcatg tccaccattt ccttgagtgt cacattgcgg
      361 ttccccaaaa tgtagcgttc gccgacccga cctttttcag ccgccaggag atgtcctttg
      421 accacgtcgc gaacatccac cagattgagt cccgtgtgga catacatcgg catttcacgc
      481 tttaaaaacc gcaggacaat atttccagtc ggagttggct tgacatcacg cgggccaatt
      541 ggggtgctcg gattgacgat gaccactggc agtccttcgc gagcagcagc gacagcagct
      601 tcttcggcca ggaactttga ttttttatag gcgctgacca gttcatcaac ggtcgtgcgg
      661 gtggtttcat ccgcttcctg accctgtggt ggcacgccga tggctgccac cgaactggta
      721 tggacaaacc gtcgaatatt ggtttttcga gcggctcgaa gcagattttt ggtcccatca
      781 acgttgcttc gataaatagc cgcactgtca cgccgaagca ggctataatg agccgccacg
      841 tgaaaaacca gttggcaccc atccagagcc cggtgtaaaa agtgttcgtc gtttaaatct
      901 ccctcaatac aatcaattgc cagtccttcc agattttttc ggtcacttcc gggtcgtacc
      961 agcgcccgga cttcactccc tgaggccagt agttccggga tgagatttcc gccgataaac
     1021 cctgtgcctc cagtcacaaa tgcacgcata gattggttat ttttctcctt cgactgaact
     1081 ttggattgac accactgaag tcacatcacc aacgattttc aaacctcagg cggagcctca
     1141 cccaaaaact atgaaaaaac tctttgcggc tgaaaacatc atgaatctgc tactggtctt
     1201 tgtgccggtg gcaatgattt tggaatgggt gatccatgcc caacccatct ggatttttgt
     1261 ggtatcgtgt ctggcgatta ttccactggc tggcttgatg ggaaaggcca ccgagcacct
     1321 ttcggaaaaa ttaggggaag ggattggcgg tttgctcaat gccacctttg gcaatgcggc
     1381 ggaattgatc atcgcgttta tggcgctccg cgaaggcttg tatgatgtag tcaaagcctc
     1441 gattacgggt tcaattatcg gtaacctgtt gctggtcttt gggttgagtg ctctggttgg
     1501 cggattgaaa tttccaaatc aaacgtttaa c
//
LOCUS       DAOQDO010001826         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93465_length_1531_cov_1.964770, whole genome shotgun sequence.
ACCESSION   DAOQDO010001826 DAOQDO010000000
VERSION     DAOQDO010001826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93465_length_1531_cov_1.964770"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKZ53_22985"
     CDS             complement(<1..1038)
                     /locus_tag="PKZ53_22985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ43370.1"
                     /translation="MPPKVVVIAGPLRGATYELSSPELLIGRSSACGIAVSDPLLSRH
                     HACLANKEGQIWLIDLNSRNGVFVNGLPIQKRLLKNGDHVVMGRSFMVYLNEEESPDL
                     DEKGEEASLNATQSRTRTETKEDLFPQIIATALELVPADRAALFLASDERGFLQFACS
                     WDRRQSSPTPFEVDPKIIHQAFHEAKPVVHNQLASAGIMLGNGQILNAQLCVPFVSPK
                     PIGVLYLDTTDELIAYSPRDIKCAMAVADLAAVAVKRLWKFRQMVEDQRKLQAELETP
                     RGMVGESQAMQQVYQFVNKVAKTDSTVLILGESGTGKELVARELHANSPRASQPFVAV
                     NCAAIPETLLES"
BASE COUNT          407 a          369 c          362 g          393 t
ORIGIN      
        1 gctttcgagc agcgtttcgg ggatcgccgc gcaattcacc gccacaaatg gctggcttgc
       61 acgtgggctg ttggcgtgca attcacgggc aaccagttcc ttgccggtcc cgctttcacc
      121 caaaattaaa acggttgaat cggttttggc caccttgttg acaaattgat acacctgttg
      181 cattgcctga ctctcgccga ccatcccgcg aggcgtctcc agttcggctt gcagttttcg
      241 ctgatcctca accatttgcc gaaatttcca gagccgcttg accgccactg ccgccaggtc
      301 agcaactgcc atggcacact tgatatcgcg gggcgagtag gcaatcagtt catctgttgt
      361 atcaaggtag agaaccccaa tcggcttagg ggaaacaaac gggacacaca attgcgcgtt
      421 cagaatttgc ccattcccaa gcatgattcc agcgctcgcc agttgattgt gcaccactgg
      481 tttggcttcg tgaaaggcct gatgaataat ttttgggtct acttcaaacg gtgtgggtga
      541 actttgtcgg cgatcccagg agcaggcaaa ttggaggaat ccgcgttcgt cactggctaa
      601 aaacaaagca gcgcggtcgg cgggtaccag ttccagggcc gtagcgataa tctgcggaaa
      661 aaggtcttct ttcgtttcag ttcgcgttcg gctttgggtc gcattgagcg aggcttcttc
      721 acccttttca tccagatccg gtgactcttc ctcattgaga tacaccataa atgagcggcc
      781 catcacaacg tggtccccgt ttttcaacag tcgtttttga atggggagac cattgacaaa
      841 cacaccattt cgactgttga ggtcaatgag ccagatttgg ccttctttat tggccaggca
      901 ggcatgatgg cgcgaaagta acggatctga aacagcaatg ccacaggctg aactccgacc
      961 aatcaggagt tccggtgaac tcaactcata ggtagccccg cgcaggggac cggcaattac
     1021 cacaactttt ggaggcatag ggagggggtg agacacccac tggatatctg gtctccccaa
     1081 ttcaggtgag caccagcttc ccaatgggaa ctaaagtaaa aaaataattc taaattaaaa
     1141 ttaaattttt tgaattaaat tgaattcgaa tttaattcaa accagtgact tgaaagtaca
     1201 ttgtacccaa aagcaaagaa actttaacct aaaagtggaa aattagcgaa aaatctcatt
     1261 caatcaacca gtcatagaag tgtaaattac ttatataaaa aacttatagc tggtcttcac
     1321 aaagataacg caggatgaaa aagatctggc acagagcttg ccctggataa gatcatctga
     1381 gttcagattt ttgaattgcc tctcctggat cggttattgg atctgaactc tttttcgctg
     1441 acttacacgg ctggataaat ggctgtaacg tgacgtcgtg taggtcagca aaactatttt
     1501 acgtgaacca catgttattc ttccggatac g
//
LOCUS       DAOQDO010001827         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93530_length_1530_cov_2.789153, whole genome shotgun sequence.
ACCESSION   DAOQDO010001827 DAOQDO010000000
VERSION     DAOQDO010001827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93530_length_1530_cov_2.789153"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKZ53_22990"
     CDS             <1..337
                     /locus_tag="PKZ53_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43371.1"
                     /translation="LTLWVLASWECTIGVGLVCGRFLRATLLLLFLQMGGTMTPMVLF
                     PQEVFISIPFVPTLEGQYIIKNLVLVSAGIVIGATIRGGRIISDPQIAQQDTQSLRAL
                     MTDGNINTL"
     gene            complement(324..1169)
                     /gene="rfbD"
                     /locus_tag="PKZ53_22995"
     CDS             complement(324..1169)
                     /gene="rfbD"
                     /locus_tag="PKZ53_22995"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNJ43372.1"
                     /translation="MNILITGAGGLLGQHLTTELKSSHRVTGYRHSDLDITKTESIAQ
                     VFDQIHPEVVVNCAAMTAVDACETESERAFQCNAIAPQLLAMACHQYAAQLVHISTDY
                     VFDGSKHGLYEPEDTPNPISVYGQSKLQGEVLVQQELPSACIVRVAGLYGTGGRNFAS
                     QLGTLLRKPGTLRSVADNRILTGYAVDVACRLRELIEGQHTGLFHVTNSGTPCSWYEL
                     ALFGAEVVGAEVQATILPVNEAELNRPAPRPMNSALGCSTSTQLGLKPLRDWKEALTD
                     YLTRY"
     gene            complement(1301..>1530)
                     /locus_tag="PKZ53_23000"
     CDS             complement(1301..>1530)
                     /locus_tag="PKZ53_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43373.1"
                     /translation="YAEIPESDVLEELLQKFIRSNGLPASPQEINRLTNMLVRRGFSF
                     DLILPRLRQLARQARLEAMSEALDTIQMNEE"
BASE COUNT          376 a          362 c          404 g          388 t
ORIGIN      
        1 gctgaccttg tgggtgctgg cgagttggga gtgtaccatt ggtgtagggt tggtgtgtgg
       61 gcggttttta cgggcaaccc ttctactgct gtttttgcaa atgggcggca ccatgacgcc
      121 catggtgttg tttcctcagg aagtgtttat ttccattcca tttgttccga ccctggaagg
      181 tcaatacatc atcaaaaacc tggttcttgt gagtgccgga attgtgattg gcgcaactat
      241 tcgtggggga agaattattt cagatccaca aattgctcaa caagatacac aaagtttacg
      301 ggcgctgatg accgatggaa acattaatac cttgtgaggt aatcagttag tgcctctttc
      361 cagtcccgca atggtttgag tcccagttgg gtgctggttg agcagccaag ggcggagttc
      421 atgggacgtg gggccgggcg gtttaactca gcttcattga ccggaagtat tgtggcctgg
      481 acttcagcgc ccacaacctc ggcgccaaac agggccagtt cataccagct acaaggagta
      541 ccgctgttgg ttacgtgaaa caacccggta tgctggcctt cgatcaactc gcgaagacga
      601 caagccacat caaccgcata tccggtcagg atgcggttat ccgccactga ccggagtgtt
      661 ccgggcttgc gcagaagtgt tccaagctga ctggcaaagt tgcggcctcc cgtgccatat
      721 aaccccgcga cccgcacaat acaggccgat ggaagttcct gctgaaccag gacttcgcct
      781 tgaagctttg attgaccata gactgaaatc gggtttggcg tgtcttccgg ttcatacaaa
      841 ccatgcttgc ttccatcaaa cacataatca gtgctgatat gaaccagttg ggctgcgtac
      901 tggtggcaag ccatcgccag cagttgtggc gcaatggcat tacattgaaa cgcacgctcc
      961 gattcggttt cacaggcatc aactgccgtc atggcggcac aattcacgac aacttcagga
     1021 tgaatctgat caaaaacctg ggcgattgac tcagtttttg ttatgtccag atcgctgtgc
     1081 cgatatcctg taactctatg agaagatttg agttcagttg taaggtgctg tccaagtaac
     1141 ccgccagcac cggtaatgag aatattcata cgaatgaaga atgaagaatt aagaattaag
     1201 aatgaagagt gaagaaacca tctggtggtt actgattaga aattaatgct ttcgacgcag
     1261 cagtcacccc gcgccaggta ccaggcactt tctcaattct tcattcttca ttcatctgaa
     1321 tggtatcgag tgcttctgac atggcttcaa gtcgtgcctg acgagccagt tgccggagtc
     1381 ggggcaagat taaatcgaat gaaaaaccac ggcggaccag catattggtc aggcggttga
     1441 tttcttgggg cgaagctggc aggccattgg agcgaataaa cttttgcagg agttcctcca
     1501 ggacatctga ttcaggaatc tcggcataca
//
LOCUS       DAOQDO010001828         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93573_length_1530_cov_2.107119, whole genome shotgun sequence.
ACCESSION   DAOQDO010001828 DAOQDO010000000
VERSION     DAOQDO010001828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93573_length_1530_cov_2.107119"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PKZ53_23005"
     CDS             complement(<1..515)
                     /locus_tag="PKZ53_23005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ43374.1"
                     /translation="MPQPIVAPTASDSDQGSVTVRYATFVLISLTVLNLLNYTDRFIF
                     AALIPYIQEDTHFTDQQFGLIGSAFTWIYTVCAPLFGYFGDRYHRGKLIAIGISIWSL
                     ATAAAGISRSFMQLLVSRSAVGIGEANYATIAPGLLSDFFTKERRGLAMSIFMAAIPI
                     GTAVGYLVGGF"
     gene            complement(662..1420)
                     /locus_tag="PKZ53_23010"
     CDS             complement(662..1420)
                     /locus_tag="PKZ53_23010"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43375.1"
                     /translation="MDKKSLSIGITVGLLIGFFFGFYWTNAMNRGFVKEAEDKLRQAQ
                     ANPAQSATSATPTGQPPNDDVHSNVTTEQATPEEIAQALDYAQKNRGNYEAQMNVGKY
                     LYFVGKKLSEARACFEDALKTKPNDMEALVTLGNVSFDEQKYDIAQKHYEAALKLNDK
                     DVNVRTDLGNTYFQRQQFDKAISFYQESLKLDPKHIPTLFNLASAQIRTNKMAEAKVT
                     IDELAKVDPQNRALPDLKAALESASSTKRDIPTH"
BASE COUNT          405 a          323 c          425 g          377 t
ORIGIN      
        1 aaaaagccgc ccaccagata gccaaccgcg gttccaatcg ggatggctgc catgaaaatg
       61 ctcatggcca gtccacggcg ttctttcgta aaaaaatcag ataagagtcc tggggcaatc
      121 gtggcgtagt tggcttcacc aattccgacg gctgagcgtg aaaccaggag ttgcataaaa
      181 ctgcgcgaaa ttcccgcggc ggcagtggcc agactccaga ttgaaattcc aatggcaatg
      241 agtttccctc gatgataccg gtctccaaaa tagccgaaca acggggcaca aaccgtgtag
      301 atccaggtaa atgcgctgcc aatcaaccca aattgttgat cagtgaaatg ggtgtcttct
      361 tgaatatagg gaatgagcgc agcaaagata aatcggtcgg tatagttgag caggttgaga
      421 acggtcagcg aaatgaggac gaaggtggca taccggacgg tcactgaacc ctgatctgag
      481 tcagacgccg tgggtgcgac aattggctga ggcatggaaa gtccttttcg aggagcgaaa
      541 tagcgaacct gaagcgagta gcaagtcgtg agtaagaagg gacaacgact gcctctgtga
      601 aggcatacag aggaaatagt tgggcacagg gttttcaaaa cgactcgcaa ctcgcttttc
      661 ttcaatgagt tggaatgtcc cgtttggtgc tggaggcgga ttcaagcgca gctttcaaat
      721 ctggaagcgc gcgattttgg gggtcaactt tggccagttc atcaatcgtg actttggctt
      781 cagccatttt gttggtccga atttgagctg aagccagatt aaagagggtt ggaatgtgtt
      841 tggggtcgag cttgagtgat tcctgataga aggagatggc tttatcaaac tgctgacgct
      901 ggaaataggt atttccgagg tcagtccgga cattgacatc cttgtcattg agtttgaggg
      961 ccgcttcata atgcttttga gcaatgtcgt atttttgctc gtcaaaggaa acatttccaa
     1021 gtgtcaccag cgcttccata tcattgggtt tggttttgag tgcatcttca aaacaggcgc
     1081 gggcttcaga cagtttcttg cccacgaaat acagatattt gcccacattc atctgggctt
     1141 catagttacc gcgattcttt tgggcataat cgagggcctg ggcaatctct tcaggggtgg
     1201 cttgctcggt ggtgacgttc gaatgaacgt catcattggg gggctgcccg gttggtgtgg
     1261 ctgaggtcgc tgattgagcc gggttggcct gtgcctgacg gagcttatct tcggcttctt
     1321 tgacaaaacc ccggttcatg gcgtttgtcc aataaaatcc aaagaaaaag ccgatgagca
     1381 ggccgaccgt gataccgatt gaaagacttt ttttatccat aacaaatgca acgtaaagaa
     1441 agaaaatgag ataagggacc cgaaagaaaa taaaatccca atgaaatcgg ggtcagggtt
     1501 cagggtttta gacgatgaag tgacgtaatc
//
LOCUS       DAOQDO010001829         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93626_length_1529_cov_3.535957, whole genome shotgun sequence.
ACCESSION   DAOQDO010001829 DAOQDO010000000
VERSION     DAOQDO010001829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93626_length_1529_cov_3.535957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKZ53_23015"
     CDS             <1..319
                     /locus_tag="PKZ53_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43376.1"
                     /translation="SNNNPAVVAMIGHTLALSGKRDEATKLLTSLQKRAKTEYVPSYS
                     LAVIAIGLGDEAAAFKYLEKSLEERYREMVFLNIEPRFDPIRQDPRFTHLLRKVGLLP
                     KGQ"
     gene            complement(460..987)
                     /locus_tag="PKZ53_23020"
     CDS             complement(460..987)
                     /locus_tag="PKZ53_23020"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNJ43377.1"
                     /translation="MSSKLVISAIGPDRVGLVKSISAFIVERNANIEDSKMAVFCGEF
                     AIIMLVGGEEADLTAIEHSQSQLAEQTGLNIFMRRPSEVKPAESGVPYTLVASSIDHP
                     GIVHRLTSELSASGINIESMETKTYSAPWSGTPMFRFEAKLSIPQQVNVPRLRSHFLS
                     LGEEENFDIDLTVIS"
     gene            1153..1446
                     /gene="gatC"
                     /locus_tag="PKZ53_23025"
     CDS             1153..1446
                     /gene="gatC"
                     /locus_tag="PKZ53_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNJ43378.1"
                     /translation="MSITKQDVEKIAVLASLELTEEEKDRLTVQMGAIVDYFGELNKL
                     DTSNVEPMSHSTLSGDPASTWRDDLMLPSLDQDAALGNAPQAGAGHFKVPRVL"
BASE COUNT          383 a          361 c          409 g          376 t
ORIGIN      
        1 gtccaacaac aacccggcag tggtggccat gattgggcac accctggcgc tttcaggcaa
       61 gcgcgacgaa gcaaccaaac ttcttacttc gctgcaaaaa cgtgccaaaa ccgaatatgt
      121 cccgtcctac agtctggctg tgattgcaat tggactcggc gacgaagcag cagccttcaa
      181 gtatcttgaa aaaagcctgg aagaacgcta ccgcgagatg gtgtttctca atattgagcc
      241 gcgatttgac ccaatccgtc aggatccacg ctttacccat ttattgcgga aagttgggct
      301 tttaccaaag ggtcagtagt cagtagtcag gtgttgggat tttgaattat ctgtcaagtg
      361 tatttggttc tatttatgca atttatgtat gcgtcttccc aggaagtcat caaactgaag
      421 cacctgggtt tggtctactg actactgact actgactcat caactaatta ccgtcagatc
      481 aatgtcaaaa ttttcttctt cgccaagcga gaggaaatgg gagcgcaggc gggggacatt
      541 gacctgttgt ggaatggaga gtttggcttc aaatcgaaac atcggcgttc cgctccaggg
      601 agcgctatag gttttggttt ccatggactc gatgttgatt ccagaggcag aaagctcact
      661 cgtcaggcga tggacaattc ccggatgatc aatcgaggac gcgaccagtg tgtagggcac
      721 acccgattcc gctggtttca cctcagacgg acgccgcata aagatgttca gaccggtttg
      781 ttcagccagt tgtgactggc tgtgttcaat agccgtcagg tcggcttctt caccgccaac
      841 gagcatgatg atggcaaatt cgccgcaaaa gaccgccatt ttgctgtctt cgatgttggc
      901 gttgcgttcg acaatgaagg ccgaaatgct tttaacgagt ccgacccgat ccggaccgat
      961 ggcgctgatg acaagtttgc tcgacatagg cttttcgtga attccttttt ttctacagac
     1021 gtgatgtggt ttgtgggagg tgcagcgtgg tgaaagcaac caatcaaact tgaatgaacg
     1081 tgccgttctg attacgctgg caaattctta actcaaaatt catttcgatg gaagccaaag
     1141 gaagcaactg caatgtcaat taccaaacaa gatgtggaaa aaatcgccgt tttggccagc
     1201 ctggaactga cagaagaaga aaaagaccgc ctgacagtcc agatgggcgc gattgtggat
     1261 tattttggtg aactcaataa gcttgacacg tcaaatgtcg agccgatgtc gcacagcacc
     1321 ttgagcggcg acccggccag cacctggcgt gatgatttga tgctaccctc gcttgatcag
     1381 gacgccgccc tgggcaatgc gccacaggcc ggtgccgggc attttaaagt gccgcgagtg
     1441 ctgtgatttt ttagggctga agaaactggg ctgagggctg aagacatcgg gctgaagact
     1501 cgcaagctcg gggctgaaga catcgggct
//
LOCUS       DAOQDO010001830         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93683_length_1529_cov_2.173677, whole genome shotgun sequence.
ACCESSION   DAOQDO010001830 DAOQDO010000000
VERSION     DAOQDO010001830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93683_length_1529_cov_2.173677"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKZ53_23030"
     CDS             complement(<1..453)
                     /locus_tag="PKZ53_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43379.1"
                     /translation="MTLPSQTSQPVSPWVDFSILSFVFLTLAGLSWAKGPDLLTDFGM
                     QTYIPYQLLAGKKLYIDIAWKYGPLSQYFNAALFWLFGESLITLYVANLIILAGITYL
                     IYKVFVEFTDRFTAVIVGCVFLSVFGMAQYVWMGNWNYVAPYVPEATHS"
     gene            complement(475..1431)
                     /locus_tag="PKZ53_23035"
     CDS             complement(475..1431)
                     /locus_tag="PKZ53_23035"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNJ43380.1"
                     /translation="MKLFVWQPIFSVFSMVCAVVLCSTCAVTGQQAPAAGAASGGETK
                     PPATRGRVEYATFQSASLGRAVNYAVSLPASYDQDKSRRYPVVIFLHGLFNSERDWEG
                     RGIEAKLQELRTAGKVGEYIVAMPYGENSFYINSKSGVRYEDAIVKDFIPFVDKSFRT
                     IAKPEHRLIEGISMGGYGALLIAFKHPELFSGVVTHCAALFEEAPKPPSSASDMRGQY
                     RYKIATELYGNPPDTSFFQENNPLYLAKTNAAKLKKLKIYFDVGSEDRYGFDVGNKLL
                     NQILTDAGVEHEFTLAPGNHGWSFLLSRGEPAFTCCWKAVKK"
BASE COUNT          421 a          385 c          376 g          347 t
ORIGIN      
        1 actatgggtc gcttcaggaa catagggcgc cacataattc cagtttccca tccacacata
       61 ttgggccatt ccaaacactg acagaaatac acatcccaca ataacggcag taaagcgatc
      121 cgtgaattca acaaatacct tgtaaatcaa ataagtaatc cctgctaaga tgatcaaatt
      181 ggcaacatac aaggtgatga gcgattcccc aaacagccaa aataatgcgg cattgaaata
      241 ctgagacagc ggtccgtact tccaggcaat atcgatatag agtttttttc cagccaaaag
      301 ttgatacggg atataagtct gcatcccaaa atcagtcagg agatccggcc ccttggccca
      361 acttagtccc gctaaagtta aaaagacaaa actgaggatt gagaaatcaa cccatgggct
      421 cacaggttgg gaagtttggg acgggagggt cataggattt tcactctttc ggtctcattt
      481 cttcacagcc ttccagcaac aggtgaaggc cggctcgcca cgactgagca gaaagctcca
      541 gccgtggttg ccgggcgcga gtgtgaactc gtgttccaca ccggcatcgg tcagaatctg
      601 attcaggagc ttgttgccca catcaaaacc gtagcggtct tcgctgccca catcaaaata
      661 aattttcaat ttcttgagct tcgcggcgtt ggttttggcc aggtagagcg gattgttttc
      721 ctggaaaaat gacgtatccg gcggattgcc atagagttcg gtggcaatct tgtaacggta
      781 ctggccacgc atatctgaag ctgatgatgg cggttttggc gcttcttcaa aaagcgcggc
      841 acagtgggtc acgacaccag aaaagagttc cgggtgctta aacgcaatca gaagcgctcc
      901 gtagccgccc atcgaaatcc cctcgatcaa gcgatgctca ggtttggcaa tcgtccgaaa
      961 actcttgtca acaaagggga taaagtcttt cacaatcgca tcttcatagc gcacgccgct
     1021 cttgctgttg atataaaagc tgttttcgcc atagggcatg gcgacgatgt attcgccaac
     1081 tttgccggca gtacgcaact cctgcagttt ggcttcaatg ccacgacctt cccaatcccg
     1141 ctctgagtta aacaacccgt gcagaaaaat caccaccgga tagcggcgtg atttgtcctg
     1201 gtcataggaa gccggcaacg aaacggcgta gttcacggct cgaccaaggc tggccgactg
     1261 aaaggtggca tattcaaccc ggccacgggt ggcgggaggt ttggtttctc cgcccgacgc
     1321 ggccccagcc gcaggtgctt gttggccagt cacggcacag gtggagcaaa gaacgacggc
     1381 gcaaaccatt gaaaagacag agaaaattgg ctgccagaca aacaacttca ttcaggtgtt
     1441 tctccttaat acagatatga ataatgtgga gagatgcgga tttgaagcga tcttgagaga
     1501 acgaagattt ttccaaagct gcccaaatt
//
LOCUS       DAOQDO010001831         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93744_length_1528_cov_3.541752, whole genome shotgun sequence.
ACCESSION   DAOQDO010001831 DAOQDO010000000
VERSION     DAOQDO010001831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93744_length_1528_cov_3.541752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..411)
                     /locus_tag="PKZ53_23040"
     CDS             complement(88..411)
                     /locus_tag="PKZ53_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43381.1"
                     /translation="MSCTVHTDHTHVHTENCGHVRVQHEDHVDYLHDGHLHHAHGDHV
                     DEHTLAVSETNPDVCAPTECGCHHDGCGHEQVPHGDHMDFLVNGRLHHVHGDHCDDHG
                     SVATQ"
     gene            complement(461..820)
                     /locus_tag="PKZ53_23045"
     CDS             complement(461..820)
                     /locus_tag="PKZ53_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ43382.1"
                     /translation="MPEHSTSCLTEHAGDALVKDSTAYLLDGTTAFQVAELFKALADP
                     TRVRIISLLAEKEMCVGEICLLLGMAQSAVSHQLRLLRTLRLVTVRKDGRHAFYVLVD
                     DHIRSLFKQGVEHIQHG"
     gene            1071..1466
                     /locus_tag="PKZ53_23050"
     CDS             1071..1466
                     /locus_tag="PKZ53_23050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43383.1"
                     /translation="MSFHSVQKTLALVFLLFVGFDLMIADMVFPHCAEHIEVCRNDIP
                     LTFDQSSGHTQVAMAEVPNQPELFSGEYSVEGTDGCDHCCFGCCSHITSTTVFTFARP
                     DVPPLVFAAEPVPLVISLWQRLYRPPKSF"
BASE COUNT          328 a          394 c          393 g          413 t
ORIGIN      
        1 atgaactggt tttctttcat ccctcatcct tcatccctca tcccttgagt tgcccccagg
       61 cccgcttttt tcagccccca ggatgtctta ttgcgtggcg acgctgccgt gatcatcaca
      121 gtggtcaccg tgaacgtggt gaagccggcc attgaccaga aaatccatat gatcgccatg
      181 cggcacctgc tcgtgtccgc acccatcgtg gtggcatccg cattcggtcg gcgcgcaaac
      241 gtcggggttg gtttcgctga cggcgagggt gtgctcgtct acgtggtcgc cgtgggcgtg
      301 gtggaggtgg ccatcgtgca gataatcaac gtggtcttcg tgttgaacgc gaacgtggcc
      361 gcagttttcg gtatgaacgt gagtatgatc ggtgtgaacg gtgcaagaca taggaaattc
      421 ctttcttttt cgtggttggt attgaacaaa cgggagtcaa tcacccgtgc tggatgtgct
      481 ccaccccttg tttaaaaaga ctgcgaatat ggtcatcgac cagaacataa aaggcatgcc
      541 ggccatcttt gcggaccgtt accagtcgga gggttctgag caaccgcaac tgatgcgaca
      601 cagcggattg ggccattcca agcagcaggc aaatttcacc gacgcacatt tccttttcag
      661 ccagcaaact gatgattcgc acccgagttg ggtcagcgag ggctttgaag agttcggcga
      721 cctggaaggc cgtggtgccg tcaagcaagt aggcggttga gtcttttacc agagcgtctc
      781 cggcgtgttc ggtcaggcag gacgtggaat gttcgggcat atgaaccttt caacatatga
      841 gcaagtgctc atatgttaat caaaaacctg gttctgtcaa accttttttt ctcaccctgt
      901 gtggcgctga aaagatatgc ctggttcaca cctcgaccac cagaggtgat cgggattgac
      961 ctgatggact gttgtaaagc aaaccaacgc ttgaactctg gactcatttt gcgtaaggtt
     1021 tgcccatcac ataaacaatt gcacctattt cagccaaccg gacacgcctc atgtcgtttc
     1081 attcagtcca aaagaccctg gcacttgttt ttctgctttt cgtcgggttt gacctgatga
     1141 ttgcagatat ggtgtttccg cattgtgcgg aacacatcga agtgtgccgt aacgacattc
     1201 ccctgacgtt cgatcagtcc agcggacaca cccaggtggc aatggctgaa gtaccaaatc
     1261 aacctgaact gttttcaggg gaatattctg tcgaaggtac cgatggctgt gaccattgtt
     1321 gttttggctg ttgttcacat attacttcga ccaccgtgtt tacgtttgca cgaccggatg
     1381 tgcctccgct ggtgtttgcc gcggaacctg tgccgctggt gatttccctt tggcagcggc
     1441 tctatcgccc accaaaatca ttctgaatca acgtcctctc tgaacaatca attttatatt
     1501 tgatgtattc aacctgtctt ttctcagg
//
LOCUS       DAOQDO010001832         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93746_length_1528_cov_3.473184, whole genome shotgun sequence.
ACCESSION   DAOQDO010001832 DAOQDO010000000
VERSION     DAOQDO010001832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93746_length_1528_cov_3.473184"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PKZ53_23055"
     CDS             complement(<1..955)
                     /locus_tag="PKZ53_23055"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ43384.1"
                     /translation="MKPVSRGQKFKLSDVTPLQSIEVGVALTFAAPQVVDVTCFGVDG
                     QEKLSDERYMIFYNQKTSPCGGLRLLGAQGGDAERFAVDLSRLPQSVQKLVFTATIDS
                     AGVMSHLTSGYLRISASGTEVARFSFSGADFAQEKAVILGELYLKDVWRFGAVGQGFN
                     GGLSALLKHFGGEEASDAAPATAPPPAAPAPPPQPAPAVNLRKVNLEKRVEKEAPKLV
                     SLVKKAAVSLEKAGLQDHQARVALCLDISGSMYKLYSSGKVQKFAERILALGCHFDDD
                     GAIDIFLFGEKAHQAGNMTLANYEGFVPRFRQTYPLEGGTSY"
     gene            complement(1043..>1528)
                     /locus_tag="PKZ53_23060"
     CDS             complement(1043..>1528)
                     /locus_tag="PKZ53_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ43385.1"
                     /translation="KVLAPLIEGEADVVYGSRFLGGDVRRVLFFWHSVGNKFLTLLSN
                     MISDLNLTDMETCYKAFRREVIQSFQLEQTRFGFEPEITIKVARRRLRVYEVGISYHG
                     RTYEEGKKIGWKDGVQALWCLLKYGFTVPVEPYHAPQSNLEGSTPATSSSPASVSVSK
                     K"
BASE COUNT          389 a          403 c          372 g          364 t
ORIGIN      
        1 cataagacgt tccaccctca agcggataag tttgtcgaaa ccgtggaaca aagccttcat
       61 agtttgccag cgtcatgttt ccggcttgat gggccttttc gccaaacagg aaaatatcaa
      121 tcgcgccgtc gtcatcaaaa tggcagccca gcgccagaat ccgttcggca aatttctgga
      181 ctttgccgga tgaatagagt ttgtacattg agcccgaaat atcaagacag agcgccacgc
      241 gtgcctgatg atcctgtaac ccggcttttt caagcgagac cgccgccttt ttgaccagtg
      301 aaaccagctt gggcgcttct ttttcaaccc gtttttcgag attgaccttg cggagattga
      361 cggcgggtgc aggttggggc ggtggagctg gagccgcggg tggcggtgct gtggctgggg
      421 cggcatctga ggcttcttca ccgccaaaat gtttgagcaa ggcactgagt ccgccattaa
      481 atccttgtcc cacggcacca aaccgccaga catctttcaa atacaattca cccaaaatca
      541 ccgccttttc ctgggcaaaa tcggcgccgg aaaagctaaa ccgcgccact tcagtgccac
      601 tggctgagat tcgtagatag ccgctcgtca ggtgtgacat tacaccggca ctatcaatgg
      661 ttgcggtaaa cactagcttt tgaaccgatt gtggaagccg gctcaggtca accgcaaacc
      721 gttcggcatc acctccctga gcaccaagca gccgaagccc accacagggt gaggttttct
      781 gattatagaa aatcatgtag cgctcatcag aaagtttctc ctgcccatca acaccaaagc
      841 acgttacatc aacaacttgc ggtgcagcaa atgtaagcgc cacaccgact tcgattgatt
      901 gaagcggcgt gacatctgac aacttgaatt tttgacctct ggaaactggc ttcatactta
      961 ttcccaaata tataagggcc tgtcagtacc acctgcgtga gcgggtggga gcgggcgacg
     1021 gacgactgac tccaaaatgg tattatttct tcgaaaccga aactgatgcc ggtgaactgg
     1081 aggttgcagg ggtgctccct tccaggtttg attggggggc gtgatagggc tcaacaggta
     1141 cggtgaatcc atatttcaat aaacaccaga gggcctgaac tccgtccttc cagccaattt
     1201 ttttcccttc ttcataggtt cgaccatgat agctgattcc gacttcatac actctcaaac
     1261 gacggcgggc gactttaatc gtaatttccg gttcaaatcc aaatcgggtt tgttcaagct
     1321 gaaatgactg gataacctca cggcgaaagg ctttatagca ggtttccata tctgtcagat
     1381 tcagatcgga aatcatgttt gacagtaagg tcaaaaattt gttcccaact gaatgccaga
     1441 agaacagcac ccggcgcaca tcaccgccta aaaatcgaga gccatagacc acatcagctt
     1501 cgccttcgat gagcggtgcc aggacttt
//
LOCUS       DAOQDO010001833         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93886_length_1527_cov_2.740489, whole genome shotgun sequence.
ACCESSION   DAOQDO010001833 DAOQDO010000000
VERSION     DAOQDO010001833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93886_length_1527_cov_2.740489"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1527
                     /locus_tag="PKZ53_23065"
     CDS             <1..>1527
                     /locus_tag="PKZ53_23065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43386.1"
                     /translation="PEFDKGWGIKLVGIQDELVGEVRPALYVLLAAVGLVLLIACANI
                     ANLLLARAMRRKSEIAIRSAIGASRWRVIQQLLVESLLLAVIGGVLGVLLARWGVDVL
                     LALSPKEIRGLAQVRLNLQVLGFTSLVTALTGVFFGLMPALEVSKLRVIEILKRQTGG
                     GIGSPHSRRFRSGLVVVQVALAVLLLIGSGLLVQSFLRLESVDPGFNQKGLLTAKIQI
                     PMSRYAEADQRIAFYNQLVERVKALPGVKSASAISFLPFTGLVAGTGFTIESRPAPPA
                     GQEWITEVELITPEYFKTMETSLVQGRMLTEAEGQKASNVVIINETMARQFFPNENPI
                     GKRLLIEMSDPIIPTEIIGVVKDSKNKGLDRNPRPMAYWAYPQLAFPFMTLVIRADND
                     PLQLIGALQREVSDLDQNLPLADVQTMEMLLAESIARSRFAAFLLTLFAGIALLMSVV
                     GVYAVVAYTTAEQTREIGIRVALGAESRDVLKLILGQGMGLVLAGIALGTAAAAGFSQ
                     V"
BASE COUNT          323 a          359 c          456 g          389 t
ORIGIN      
        1 tcctgagttt gacaaaggct ggggcatcaa actggttggc attcaagatg aactggtcgg
       61 agaggttcgc ccagcgttgt atgttttgct ggcggcggtt gggttggtgc tgctgattgc
      121 ctgtgccaac attgccaatt tgctgctggc ccgagcgatg agacgcaaat cggaaatagc
      181 aatccggagc gcgattggcg ccagtcggtg gcgggtcatc cagcaattgc tggtcgaaag
      241 tctcttgctg gcagtgattg gcggtgtgtt gggggtgttg ctggcgcgtt ggggagtgga
      301 tgtgcttctg gcactgagtc cgaaagaaat ccgtgggttg gcccaggtgc ggcttaatct
      361 gcaggtactt ggatttacgt cactggtaac tgccctgacc ggtgtgtttt ttggattgat
      421 gccggcgctt gaagtatcga aattgagggt gatcgaaatt ctcaaacggc agaccggagg
      481 cggtatcggg tcgcctcaca gtcgtcggtt tcgttctgga ttggtggtgg tacaggtggc
      541 actggccgtt ctgttgctga ttggttccgg gttgctggtc cagagttttc tccgacttga
      601 atcagttgat ccaggattta atcagaaagg attactcacc gctaaaatcc agattcccat
      661 gtccaggtat gccgaagcag accagcgtat tgccttctac aatcaactgg ttgaacgcgt
      721 aaaagcgctg ccaggtgtga aatcagccag tgccatcagt tttctcccct ttaccggtct
      781 ggttgccggc accggcttca cgattgaaag tcgcccggcc ccgcctgctg gacaagagtg
      841 gattaccgag gttgaactca ttaccccaga gtatttcaag acgatggaaa catcactggt
      901 tcagggacga atgttgaccg aggctgaagg ccagaaagcc tccaacgtcg tcatcatcaa
      961 tgaaaccatg gcccgccagt ttttcccgaa tgaaaacccg attggcaaac ggttgttgat
     1021 tgaaatgagc gacccgatta ttcccaccga aatcatcggc gtggtgaaag attcgaaaaa
     1081 caaagggctg gatcgcaatc cacgaccaat ggcctactgg gcgtatccgc aactggcttt
     1141 tccctttatg acgctggtga ttcgggctga caatgaccca ctccaattga ttggtgccct
     1201 gcagcgcgaa gtgtcggacc ttgatcaaaa cctgccgctg gccgatgtcc agacaatgga
     1261 aatgctgtta gcggaaagta ttgcccggtc acggtttgcg gcttttttac tgacgctttt
     1321 tgcagggatt gccctcttga tgtcagtggt tggagtctat gccgtcgttg cgtacacaac
     1381 ggctgaacag acgcgggaaa ttggtattcg agtcgctctg ggcgccgagt cccgtgatgt
     1441 attgaaactg atattggggc agggaatggg tctggtgctg gctggtattg cgctcggaac
     1501 tgcagccgca gccggatttt cacaggt
//
LOCUS       DAOQDO010001834         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93892_length_1527_cov_2.596467, whole genome shotgun sequence.
ACCESSION   DAOQDO010001834 DAOQDO010000000
VERSION     DAOQDO010001834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93892_length_1527_cov_2.596467"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKZ53_23070"
     CDS             <1..938
                     /locus_tag="PKZ53_23070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ43387.1"
                     /translation="CLLALGQADPPADELIVVTDGDTQGSGDLAKSCGATVYSTSVPS
                     GPAAARNLGASQASGDIVFFVDADVVLHPDAIGRVHTTFEQFPEIVAVFGSYDTQPGA
                     PNFLSQYRNLLHHYVHQTACEDASTFWSGCGAIRRSVFLEVGGFDARTYQTPCIEDIA
                     LGYHLRAQGFHIRLDKQLQCRHLKEWRPWSMLKADFWYRAVPWTLLILRSNGFVNDLN
                     VQTSSRLCVALTFLLMLSAGSGWWWPVMWAVTMVCALVLFVVNRELYLFYYHLRGFPF
                     TFLAVFWHWLYFFYSGLAFLFGCMLHLFNLSKPSL"
     gene            997..>1527
                     /locus_tag="PKZ53_23075"
     CDS             997..>1527
                     /locus_tag="PKZ53_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNJ43388.1"
                     /translation="MTDHFPTVIIGAGPAGLTAAYELVRNGMRPLVLEQGSQVGGIAR
                     TEVYKGYRFDIGGHRFYTKVKEVEDLWREMMGDDFIKVPRLSRIYYKGRFYQYPLSFL
                     NTLTNLGLTESFLVLMSYCKARITPHRDEQTFEEWVTNRFGSRLYRTFFQAYTEKVWG
                     IPCNKIQAEWAAQRIQN"
BASE COUNT          315 a          376 c          414 g          422 t
ORIGIN      
        1 aatgtttact tgcacttggt caggcagacc ctcctgccga tgaactgatt gtcgtgacag
       61 atggtgatac ccaagggtca ggtgatctgg caaaatcatg tggggctacg gtctattcaa
      121 cctcagtccc ttcgggccca gccgcagcgc gaaatctggg cgccagccag gcgagcggcg
      181 acatcgtctt ttttgtggat gccgatgtgg tactgcatcc ggatgcaatt ggacgggttc
      241 acacgacgtt tgagcagttc ccggaaattg tggcggtgtt tggttcctat gacacgcagc
      301 ccggagcgcc caactttttg tcgcaatacc gaaatttgct tcatcactat gttcatcaaa
      361 ccgcctgcga agacgcctcg accttctgga gcggctgtgg cgccattcgc cggtcagtgt
      421 ttttggaggt cggcggtttt gacgcccgaa cctaccagac tccctgcatc gaagacatcg
      481 cgcttgggta tcacctgcgg gcgcaagggt ttcacatcag acttgataaa caactgcagt
      541 gccggcactt aaaagaatgg cgtccctggt ctatgctgaa agccgatttc tggtatcggg
      601 ctgtgccctg gacgctgctt attttgcgtt cgaacgggtt tgtcaatgat ctcaatgttc
      661 agacctccag ccggttgtgc gtggcgctga catttttgtt gatgttgagt gctggcagcg
      721 gctggtggtg gccggtgatg tgggctgtga caatggtttg tgctctggtg cttttcgtgg
      781 taaacagaga attgtatctg ttttattacc acttgcgcgg attcccgttc accttccttg
      841 ctgttttctg gcactggttg tacttttttt atagtggact tgccttcctc ttcggatgta
      901 tgctccacct cttcaatctg tccaagccct cactctgatt ttcaccttac cgcctgtgtg
      961 cggtcatttt tttcaagaaa gattcaataa accggaatga ccgatcattt cccaactgtc
     1021 attattggtg ccggaccggc gggacttacg gcggcgtacg aacttgtccg taacggaatg
     1081 cggccattgg tgttagagca aggtagccag gtgggcggta ttgcccggac cgaagtctat
     1141 aaaggatatc ggtttgatat tggcggccat cggttttaca caaaagtcaa agaggttgaa
     1201 gacttgtggc gcgagatgat gggcgatgac tttatcaaag tgccccgact gtcgcggatt
     1261 tactacaagg gccggtttta tcaatatccg ctcagttttc tgaacacgct caccaatctt
     1321 gggttgaccg agagctttct ggttctgatg agctactgta aagcccggat tacgccccat
     1381 cgcgatgaac agacctttga agagtgggtg accaatcggt ttggttcgcg gctctatcgc
     1441 accttctttc aagcttacac agaaaaagtc tggggcattc cctgcaataa aattcaggcc
     1501 gaatgggcgg cgcaacggat tcaaaat
//
LOCUS       DAOQDO010001835         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93931_length_1527_cov_2.063859, whole genome shotgun sequence.
ACCESSION   DAOQDO010001835 DAOQDO010000000
VERSION     DAOQDO010001835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93931_length_1527_cov_2.063859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKZ53_23080"
     CDS             complement(<1..425)
                     /locus_tag="PKZ53_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ43389.1"
                     /translation="MKQRKLGKNGLVVSALGLGCMGMSHAYGVPDDAESMRVLHRYVE
                     LGGNFLDTAEMYGPYKNEELLGRFLREVPREKLVIATKFGFRIDPQTGTATGLDSSPD
                     NIRKVCDDSLRRLGIETIDLFYQHRVDPAFPIEETVGVMA"
     gene            681..>1527
                     /locus_tag="PKZ53_23085"
     CDS             681..>1527
                     /locus_tag="PKZ53_23085"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNJ43390.1"
                     /translation="MRRTGQVCLLIGFMILFIGGILWHFQSTVQADDPIDPGGGMLII
                     LDSAGNPGASCPLRKTEVSASISGPIARVNVTQIFYNPTNQPVEAIYLFPLSNHAAVD
                     GMKIHIGNRQIEGILTRREEAERLYETARRSGRTAALLTQERPNVFTQSVANIGPQEV
                     IRVEITYAETLTYENGQYQFVYPTVVGPRYQPALSSEPALPQPPVLPTNLSTPNDLTM
                     SITVDAGQLIQQLSSPSHDISTSFTPERQTAIIHLNPATALANRDFVLNYRVAAVEIS
                     DVFLAH"
BASE COUNT          374 a          417 c          342 g          394 t
ORIGIN      
        1 gccatcacac caacggtttc ttcaattggg aaagccgggt ccactcgatg ttgatagaac
       61 aaatctatgg tttcaattcc cagtcggcgc aggctgtcgt cacaaacctt gcgaatgttg
      121 tctggtgaac tatccagacc cgtcgctgtc ccggtttggg gatcaatccg aaaaccaaat
      181 ttggtggcaa tgacgagttt ttcacgtggg acttcgcgca gaaaacggcc caataattct
      241 tcgtttttgt aaggtccgta catttcggcg gtatcaagga aatttccgcc aagctccaca
      301 tatcggtgga gaacccgcat ggattcggca tcatcaggca caccgtaggc gtgtgacatt
      361 cccatacagc ccaggcccag tgccgagaca accagaccat tttttccaag tttgcgctgc
      421 ttcatcaact gtctcctttg atcactattt ctgaaaactg agaatgtctg gtcaacagaa
      481 agttattgcc agaccagagg ttttgattca gttccccaac tatggtgcca ccctccggcg
      541 agtgcaaatc atctttcctt cttctttttc tccaggccca cctgatggaa ctgtaactgg
      601 attccaggaa ctaatttccc cacctggtgt ccaactcgac caatgcttcc gaacaaatca
      661 cacattggag gtagacggct atgcgtcgaa caggacaggt gtgtcttctg attgggttta
      721 tgatcttatt tattggagga atactctggc attttcagtc aacggttcag gctgacgatc
      781 ccattgatcc aggtggtggg atgttgatca tcctcgatag cgcggggaac ccaggtgcca
      841 gttgcccgct tcgtaaaacc gaagtctctg ccagcatcag cggtccaatt gcgcgagtga
      901 acgttaccca aattttttat aaccccacca atcaaccagt tgaagccatt taccttttcc
      961 ccctctcaaa tcatgcggca gttgatggga tgaagataca tatcgggaac cgtcagattg
     1021 aaggtattct gactcgtcgt gaagaagctg aacgactgta tgaaactgcc cgccgatctg
     1081 gccgaactgc ggccctgctg actcaggaac ggcccaatgt gtttacccaa tcggtcgcca
     1141 atattggccc gcaggaagtg attcgggttg aaattacgta tgccgagacc ttgacctatg
     1201 aaaatggtca gtaccagttt gtctatccaa cggtggttgg cccacggtac caaccggcat
     1261 taagttcaga accagctttg cctcaaccac ccgttctgcc aacaaacctc agcaccccca
     1321 atgatcttac aatgtcaatc acagttgatg ccggtcaact gattcaacaa ctttcttctc
     1381 ccagccatga catcagtacc tcgtttactc ctgaaagaca aaccgccatc attcacctca
     1441 acccggctac ggcgttggca aatcgggatt ttgtcctcaa ctaccgggtt gcggcggttg
     1501 aaatctcaga tgtgttcctt gcccacc
//
LOCUS       DAOQDO010001836         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93953_length_1527_cov_1.616168, whole genome shotgun sequence.
ACCESSION   DAOQDO010001836 DAOQDO010000000
VERSION     DAOQDO010001836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93953_length_1527_cov_1.616168"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKZ53_23090"
     CDS             complement(<1..227)
                     /locus_tag="PKZ53_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43391.1"
                     /translation="MKRYTGLSIGVLWGVVLFLALPFFVTRATHLSGTNEAAAHSPAH
                     RLTVSPRAYSLLRESARLAEPRLPAAVRQAVT"
     gene            644..>1527
                     /locus_tag="PKZ53_23095"
     CDS             644..>1527
                     /locus_tag="PKZ53_23095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ43392.1"
                     /translation="MPALSSSGSQSVPLISVVVATRNRGDQVRQTLISILANDYPRFD
                     LWVVDQSESDDTSQTVQPFETDGRLHYVRSSTKGVSIGRNLGIALSQGELIAITDDDC
                     EAAPDWISQMVNAFSEQPSAGIVFGNVLTSPHDTNAGFIPGYHRSNCFLARTIYYKHC
                     VEGISACMGLRRSVWHQLNGFDPLLGAGAPLKSAGETDFAVRALLARIPIYETPAPAV
                     THFGFRTWEQGRSLIQGYMFGIAAALTKNLKCGHWSVLAVLVHLGWRWAFQKPVVDLG
                     RRPPRWMRLAAFLRGIWRA"
BASE COUNT          343 a          401 c          411 g          372 t
ORIGIN      
        1 gtgaccgcct gccggaccgc cgctgggagt cgaggttctg ccaatcgggc gctctcacgc
       61 aacagcgaat aggcgcgggg ggacactgtc aaccgatgcg cgggcgaatg ggctgccgct
      121 tcattggttc cactgagatg cgtggcacgc gtcacgaaaa acggaagtgc cagaaataac
      181 accactcccc agagaacacc gattgacaac cctgtatacc gtttcatcat tgactccagt
      241 tcattttcca tgaacccgct cccacccgct cacgcaggtg gtactgacta gcccccacgg
      301 gaggcgcaca aacgctgatt ccccgttgtt tttaccggta gacagcaaaa ctgcgcacta
      361 aacagtcttc ggttggcctg atgaaggtcg gcaattgcca gacacaccgc gcctgaccgg
      421 gttgagagac tcccgtaaat gaatattgtt cgcgatggta gctgtaacaa aatagatttg
      481 tcttgtgggg agacacggta aagaatcagg ttactgaacg tcaagtcaaa agttaaaatt
      541 ttttcagttg agtgaaagat tttttatgct tcaatatgga gtgcggtggc ttgacaccgc
      601 ttttgctcta ttgccgaatt cgagaagcaa tcaggaaatt ctcatgccag ccctctcttc
      661 ttctggttct caatcagtgc cgctgatttc agtcgtggtt gccacccgca atcgtggaga
      721 ccaggtgcgg caaacactca tctcgattct ggccaacgac tatccccggt ttgatctgtg
      781 ggtcgtggac cagagtgaat cagatgacac cagccagaca gttcaaccat ttgaaacaga
      841 tggccggctg cattatgtca gaagttcaac gaaaggcgtc tcaattgggc gcaatctggg
      901 aattgccctc tctcaaggcg aattgattgc cattactgat gatgattgtg aggcggcccc
      961 tgactggatc agccagatgg tgaacgcctt ttccgaacag ccctccgccg ggattgtctt
     1021 tggaaatgtt ttgacgtcgc cacatgatac aaacgccgga ttcatccctg ggtatcaccg
     1081 aagtaattgt ttcttagccc gtacaatcta ttacaaacat tgtgttgaag gaatttcagc
     1141 ctgcatgggc ctgcgtcgct cagtgtggca tcaactcaat gggtttgatc cgctgctcgg
     1201 tgctggagcg ccgctcaaat cggctgggga aactgacttt gccgttcggg cgctgctggc
     1261 caggattcca atttatgaaa ccccggcccc ggcagtcacc catttcgggt ttcgcacctg
     1321 ggagcaggga cgaagcctga ttcaaggata tatgtttggg attgctgcag ccctaaccaa
     1381 aaacctgaag tgtggtcact ggtctgttct ggcggtcctg gttcatcttg gctggcgatg
     1441 ggcgttccaa aaaccagtgg ttgatctggg acggcgtcca ccccgctgga tgcggctggc
     1501 ggcttttctc cgcggtattt ggcgggc
//
LOCUS       DAOQDO010001837         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_93984_length_1526_cov_3.248810, whole genome shotgun sequence.
ACCESSION   DAOQDO010001837 DAOQDO010000000
VERSION     DAOQDO010001837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93984_length_1526_cov_3.248810"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKZ53_23100"
     CDS             complement(<1..881)
                     /locus_tag="PKZ53_23100"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ43393.1"
                     /translation="MRRKLTQLLSALLFLAVVLSPTGFSQPVLAYAGPAVAGGAQEVS
                     GSIKGVVRDQTGAVVAGATVTAKNAQRTYEATTDASGNYTFANLQPGTYTISATASGF
                     TTYEVSDLSLELGRTLQVNMDLKAGGQDEIVNVTAADAAYVDVTSSKTAVNINEMQIR
                     VLPKTLNFSSVLEVAPGTRSEGKSGGFQIDGASGVENVFVVDGVEVTEITQGRLRSSK
                     NTPLDFVKEVQVKSAGYEAEYGGATGGVVNVVTKSGTNEFHGEGRFEYTSDSFRAEDN
                     PTQRLNPLDPRQQTVEY"
BASE COUNT          398 a          397 c          350 g          381 t
ORIGIN      
        1 aaatattcaa ccgtttgctg gcgtggatcg agtggattta accgctgggt cgggttgtct
       61 tccgcccgga atgaatcaga ggtgtactca aaccggcctt cgccgtggaa ttcgttggta
      121 ccgctcttgg tgaccacgtt gaccacacca ccggtcgcgc cgccgtattc agcttcatag
      181 ccagcgctct tgacctgaac ttctttcaca aagtccagtg gcgtattttt tgagctgcgc
      241 agacggccct gggtgatttc agtcacttcc acgccatcca ccacaaacac gttttcgacg
      301 cccgaggcac cgtcaatctg gaaaccacct gattttcctt cgctccgggt cccaggagcg
      361 acttcgagaa ccgacgaaaa attcagtgtt tttggcaaca cccgaatctg catttcattg
      421 atgttgaccg ctgtcttgga tgaagttacg tccacatagg ccgcatcggc ggctgtcacg
      481 ttgacaattt catcctgacc acccgctttc aggtccatat tgacctgcag cgtccgaccc
      541 agttccaatg acaggtcact cacttcatag gtggtaaacc cggaagcggt ggctgagatg
      601 gtataggttc ctggctgaag gttggcaaaa gtgtaatttc cggacgcatc ggtcgtggct
      661 tcatacgtac gctgagcgtt tttggcggta accgtcgctc cagccaccac agcaccggtt
      721 tgatcccgga caacaccttt gatacttcct gatacttcct gggcaccacc cgcaaccgct
      781 ggtccggcat aggccagcac cggctgcgaa aatcctgtcg gtgacaggac aacggccaga
      841 aataacaggg cggaaagcaa ttgagtcagc tttcgtctca cgttttattc ctcccagaat
      901 gcttatcgtt ttttgttctg tgatcgccca tcactgtggc ctgcgggtaa agcaggcgca
      961 cttcacggct tgaagaggtc agatgccatt gagatccttt acagacttga ggggagatag
     1021 acaggtattt gcgaacaagg tgccgtgaac tgagaaggag tggcaatctt ttgggcagca
     1081 tctcgcgttc caaaacattc aattttttga atcattccta taaaaaccag acccagaaac
     1141 atcgcccagg ggtttccact gcattggctt gattgcaaaa gtccagcatt gaaacaccat
     1201 cactctgatc aaaagtgctt ttaattcaaa attttgaaat aactaaaacc cacacataaa
     1261 aacccgtaac agaaatcagg cattcaggtg aaaaaaatta tgcaaacaga tgaatttttt
     1321 gacaaatcat cacgtcaggg cgttcaaaat cgcaagaata tccaaaatta tgaaaagaat
     1381 atccaaaatt atagatttct aatctgagat tacgaaggcc gcactcgact cgccaggcat
     1441 gaattaaaat tccaaaaaaa tggatttcct ggttctctca ccagtcgaaa cctggcaatc
     1501 ccggtctctg gtgcagccat ttgagg
//
LOCUS       DAOQDO010001838         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94236_length_1524_cov_2.567733, whole genome shotgun sequence.
ACCESSION   DAOQDO010001838 DAOQDO010000000
VERSION     DAOQDO010001838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94236_length_1524_cov_2.567733"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..705
                     /locus_tag="PKZ53_23105"
     CDS             277..705
                     /locus_tag="PKZ53_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ43394.1"
                     /translation="MTRIRFHLAFPIQDIEQAKVFYRDGLGCQVGRESPVSIILNLGG
                     NQIVAHKTSEDLPEQRGIYPRHFGLIFDAEADWEAMLERAQAHHLKFYQEPKVRFPGL
                     PLEHRTFFLQDPFNNLLEFKFYRHAEAVFGAAEFSQIGDQ"
     gene            968..1486
                     /gene="rfaE2"
                     /locus_tag="PKZ53_23110"
     CDS             968..1486
                     /gene="rfaE2"
                     /locus_tag="PKZ53_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNJ43395.1"
                     /translation="MTTAGPQNCAASAKVCSLTDLVGHRAQLRAAGKRVVFTNGCFDL
                     IHPGHVRYLAEARTLGDALIVAINSDRAVRELKGPSRPILNQDERAEVLAALEAIDFV
                     TVFDDLDPRSTIVALLPDVLVKGGDWDLSQIIGREEVEAAGGLVRSLPFVPGVSTTDI
                     VSRILKGIQNEE"
BASE COUNT          366 a          373 c          379 g          406 t
ORIGIN      
        1 cgctgcccaa aatagaatca aaggtctgtc ccggtgaata ttgggaatac caactgcagc
       61 cagacggcac cccaatcctc aaattcagcc agccgattga gaattcagag ttcaagcttt
      121 aagtaccttg tcataagttt cctgagacat tggattaggt aagtatttga ttttttccgt
      181 gttttccgtg ttttccgtgg ttaaaatatc tggaattttt caggtacgtt cttgcaagct
      241 aaagcttgaa ctctgaactt tttccgaagg agtgatatga cgcgcattcg ttttcatctt
      301 gctttcccaa ttcaggacat cgaacaggcc aaagtattct atcgtgatgg cttagggtgc
      361 caggtcggac gcgaaagccc ggtttcgatc atcctcaatc tggggggaaa tcaaattgtt
      421 gcccacaaaa ccagcgaaga cttgcccgaa caacgcggta tttatccacg tcatttcggg
      481 ttgatcttcg acgccgaagc cgactgggaa gccatgctcg aacgggcgca ggcgcatcac
      541 ttgaagttct atcaggagcc aaaggtccgc ttcccaggcc tgccactcga acatcggacg
      601 ttctttctcc aggatccatt caacaacctg ctggagttca aattctatcg ccatgctgaa
      661 gctgtgtttg gcgctgcgga attttcccag attggcgacc agtaaacgaa cagggctcag
      721 ggttcagggt tcagggaaat acaagttttt tgaatgattt agcctgttgc tcatccgatt
      781 ggatcatttc attacggcat tcttccgtat gctcattttg ttcattttat ccattcaaaa
      841 accctgaacc ctgaaccctg aaccctgaac cctgcatctt gaatcccaaa ggtgtgggtt
      901 atgcttgaca gtgcggcgta aacccttcta cattcgctgt tttgcatcac tttcaggaga
      961 tttcgacatg acgactgcag gtcctcaaaa ctgtgcagcc agtgccaagg tctgttcact
     1021 gactgattta gtcgggcatc gcgctcaact tcgggcggct ggcaagcgcg tggtatttac
     1081 taacggctgc tttgatctga tccatccggg gcacgttcga tacctggctg aagcccgtac
     1141 ccttggggat gcactcatcg tggcgattaa cagtgaccgg gcggtgcgcg aactcaaagg
     1201 ccccagccgt ccgattctca atcaggacga acgcgctgaa gtcctcgcgg cacttgaagc
     1261 cattgatttc gtcacggtgt ttgatgatct cgatccacgc tccacgattg tggcgctgtt
     1321 gccagatgtg ctggtgaaag gcggcgactg ggatctcagc cagattattg gccgtgaaga
     1381 agtcgaagcc gctggcggtc tggttcgttc gctcccgttt gtccccggcg tctcaacgac
     1441 agatattgtt tcaaggattt tgaaaggaat acagaatgaa gaatgaagaa ttgagaatga
     1501 agaatgaaga attgagaatg aaga
//
LOCUS       DAOQDO010001839         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94336_length_1523_cov_4.014305, whole genome shotgun sequence.
ACCESSION   DAOQDO010001839 DAOQDO010000000
VERSION     DAOQDO010001839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94336_length_1523_cov_4.014305"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>1523
                     /locus_tag="PKZ53_23115"
     CDS             196..>1523
                     /locus_tag="PKZ53_23115"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ43396.1"
                     /translation="MSVSFFQKLSQFQTEKLAPTWHGILTRIGFILLIVAIMGYLRAE
                     RILTVDNNLGGYAIVSLGFILLVAHNLSIVLAVIGLPHVTSYILTGVMCGPFVFNLLA
                     EPLVKDLSLVNNLALSLIAFIAGGELRLRTLRERWKSVLFISLFETAFVFLLCSSSFW
                     FLSRYIPFTAGTSNAFRLAVSLVFGGYIIVNSPAVTIGVINEYRPKGPVSQTVLGVVV
                     VKDIIVVLIFSLMVSLAKVLTNPEIKFEIVSFLLHFGWELLVSSCLGAAVGVVVAIYL
                     KYIKTDLILFTVGVGLLCYQTAKALHLDGILLCLVAGFFVENFTRQGETFIQSIEHTL
                     GVVFAIFFSVAGAKMNLDALQSVWYFALFFVTVRLLAIYCGSRIGAKLGKAALRVEQH
                     AWLGLVSQAGVALGLAVVIEQTFPNWGTNLSTIILALIAMFELIGPLAFR"
BASE COUNT          316 a          396 c          372 g          439 t
ORIGIN      
        1 ggaacccgga acccggaacc cggaacccgg aacccggaac ccggaaccct gaattaaaat
       61 gcacccatct attgcgcaat ttacaactat cggttagata ccaaacaaca cccctcacta
      121 caccacgtcc caacgaacca attcagtaca ttccatcagg ccactcaagc cgcagacatc
      181 ctgaggaggc acaccgtgag tgtttcattc tttcagaaac tgtctcagtt tcagaccgag
      241 aagctcgccc caacctggca cggtattttg actcggattg ggtttatttt gctgattgtg
      301 gcaatcatgg ggtatctccg ggcggaacga atcctcaccg tagacaacaa tcttggtggc
      361 tatgctattg tttcacttgg atttatcctg cttgtcgctc acaatctgtc aatcgttcta
      421 gcggtcattg gattgcctca tgtcacatcg tacattctca ccggagtaat gtgcggacca
      481 tttgtattta atcttctggc cgagccactg gtcaaggatt taagtctggt caacaacctg
      541 gccctcagtc tgattgcttt tatcgccggt ggtgagttgc ggttgcgaac cttgcgcgag
      601 cggtggaaat ccgttctctt catcagcctg tttgaaacag cctttgtctt tctcctgtgt
      661 agctccagtt tttggtttct ctcacgttat attcccttca ctgccggtac ctccaatgcg
      721 tttcgactgg cagtttcgct ggtctttggc ggctacatta ttgtcaattc cccagccgtc
      781 actatcggtg tgatcaatga ataccgcccc aaaggtccgg taagccaaac cgtgctgggc
      841 gtggtcgtgg tcaaagacat tatcgtcgtg ttgattttca gtttgatggt gtcactggcc
      901 aaagtactga ccaacccgga gatcaaattt gaaattgtat cttttttact tcacttcggc
      961 tgggagctac tggtgtcaag ttgtcttggt gcagccgttg gtgtggtggt ggcgatttac
     1021 ctgaaataca tcaagaccga cctcatcctc tttacagtcg gggttggcct gttgtgttat
     1081 caaaccgcca aagccctgca cttagatgga attctgctct gtctggtcgc ggggttcttt
     1141 gttgaaaact ttacccgtca gggagagacc ttcattcaaa gcattgaaca cacgctgggc
     1201 gtcgtgtttg ctattttctt tagcgtggct ggtgccaaga tgaatctcga tgcactgcag
     1261 tccgtctggt actttgctct gttttttgtc acagtgcgct tattggccat ttactgtggg
     1321 agtcgaattg gcgccaaact cggcaaggca gccctgcggg ttgagcaaca tgcgtggctg
     1381 ggattggttt cacaggccgg ggttgcactg ggtctcgccg tggtgattga acaaaccttc
     1441 cccaactggg gcaccaatct gagcacgatc atcctggcct tgatcgccat gtttgaactg
     1501 atcggcccgc tcgcttttcg gta
//
LOCUS       DAOQDO010001840         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94369_length_1523_cov_2.611717, whole genome shotgun sequence.
ACCESSION   DAOQDO010001840 DAOQDO010000000
VERSION     DAOQDO010001840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94369_length_1523_cov_2.611717"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1523
                     /locus_tag="PKZ53_23120"
     CDS             <1..>1523
                     /locus_tag="PKZ53_23120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ43397.1"
                     /translation="ERDLRRTPLLQVMFSFESASHRPPRPEALAGETELLKINPLEIN
                     LAIDRGTSNLDLTVFFRETAEGIASTWEYNRELFESATIRRFAQILEIILETVVTNPD
                     LPITEIPLLSEAELRRVTVDWNRTHTDFPLETPVHLLFEQQVKRTPFAIAVTYHGQSL
                     SYRELNQRATQVASHLASLGVGIESLVGLCLDRSLEMVIGILGILKTGAAYVPLDPVY
                     PKERLAWMIQDARLTSILTTTHMRNRLPEQIPNVIALDNQFFPPSVFFQSPIPDPENL
                     AYVIYTSGSTGLPKGVLIPHRGLTNLTLAQIKAFGLTGKSRVLQFASLSFDASVSEIF
                     TTLLAGATLILASKEDLRPGPELIDLLHREQISTVTLPPSVLAVLPDAELPHLHTLVT
                     AGEACSSDIVHRWGCNRRLLNAYGPTETSVCATIHHCTSTNRRPPIGRPIDNVQVYVL
                     DHAQQPLPVGIPGEIYIGGMGVARGYLNRPDLTEERFQPGFRVPGSGVRVPGFRNPF"
BASE COUNT          336 a          447 c          377 g          363 t
ORIGIN      
        1 cagagcgtga tttgcgtcgc accccgctac tccaggtcat gttttcattt gaatcggcca
       61 gccatcggcc acctcgaccc gaagcactgg ccggtgaaac cgaacttttg aagatcaacc
      121 cgcttgaaat caacctggcg attgatcggg gaacatcgaa cctggatttg acggtctttt
      181 tccgcgagac agccgagggt atcgcatcga cctgggagta caaccgggaa ttatttgaaa
      241 gcgccacgat acgacgcttt gcccagatac tcgaaattat tcttgaaaca gttgtcacca
      301 atcccgatct gccaattacc gaaattccgc ttctctcaga agcagaactc agacgggtca
      361 ccgttgattg gaatcgaacc cacaccgatt ttccgctcga aaccccggtt catctgctgt
      421 ttgaacagca ggtgaagcgt accccgtttg ccatcgcggt tacctatcac ggacagagct
      481 tatcctatcg ggaattaaat caacgggcga cccaggtggc atcgcacctg gcttcactcg
      541 gggttgggat tgaatctctg gttgggttgt gcctggatcg ttcacttgaa atggtgattg
      601 gaatactggg aattttgaaa actggggccg cctatgtccc acttgatcca gtgtacccga
      661 aagaacggct ggcctggatg attcaggatg cccggctcac atcaatcctg accacaaccc
      721 acatgcgcaa ccggttgccg gaacagatcc ccaacgtcat tgcgctcgac aatcagtttt
      781 tcccgccatc ggtatttttt caatcgccca tacccgatcc agaaaacctg gcgtacgtga
      841 tttatacctc gggttcaacc ggtttgccga aaggggtgtt gattccgcac cgtgggctga
      901 ccaacctgac gcttgcccaa atcaaagcgt ttggcctcac agggaagtcc cgagttctgc
      961 aatttgcctc gctaagtttt gacgcttcgg tttctgaaat ttttaccacg ctccttgctg
     1021 gtgccacgct gatcctggca tcaaaagaag acctgcgacc tggccctgaa ttgattgacc
     1081 tcctccatcg cgagcaaatc tccaccgtca cgctgccacc ttccgtactg gccgttttgc
     1141 cggacgctga gttgccacac ctgcacacgc tcgtcacagc gggcgaagcc tgctcatctg
     1201 atattgtgca tcgctgggga tgcaatcggc gattgttaaa cgcctatggt ccaaccgaaa
     1261 catcggtatg tgccaccatc caccactgca catcaaccaa ccgcagacct ccgattggcc
     1321 gcccgattga caatgtccag gtttatgtcc tggaccacgc ccagcaaccg ctcccggtgg
     1381 gtattccagg tgaaatttac attggcggga tgggggttgc ccgtggctac ctcaaccgac
     1441 ctgacctgac ggaagaacgg tttcaaccag ggttccgggt tccgggctcc ggggtccggg
     1501 ttccgggttt tcgaaatcct ttt
//
LOCUS       DAOQDO010001841         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94372_length_1523_cov_2.591281, whole genome shotgun sequence.
ACCESSION   DAOQDO010001841 DAOQDO010000000
VERSION     DAOQDO010001841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94372_length_1523_cov_2.591281"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKZ53_23125"
     CDS             complement(<1..895)
                     /locus_tag="PKZ53_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNJ43398.1"
                     /translation="MHDKFREECGVFGIWGHPEAARLTYLGLYSLQHRGQESAGIVSS
                     DGNRLYSERGMGHVSEIFTEEAFRRLPGNRAIGHVRYSTAGEVSINEAQPFAIKCHWG
                     EIATCHNGNLPFASEMRRELEAEGAIFSSTSDTEVVLHKLARSRKASLYEAVVETFRS
                     IEGAYSILIQTTDCLIAVRDVHGFRPMCIGQLDEHSYVVASETCAFDLIGATYLRDVQ
                     PGEVVMINRDGIHAQSPFPEHKPAFCIFEHVYFSRPDSLVFGRSVNKSRHKMGKRLAL
                     EHPVPADIVVPIPDSGVAAAIG"
     gene            complement(953..1120)
                     /locus_tag="PKZ53_23130"
     CDS             complement(953..1120)
                     /locus_tag="PKZ53_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43399.1"
                     /translation="MHFLLMVLFAAFVSAVFAGVSSEVSTPKARFLYGLRVFLSFIGI
                     GLALSWLMYLL"
     gene            complement(1265..>1523)
                     /locus_tag="PKZ53_23135"
     CDS             complement(1265..>1523)
                     /locus_tag="PKZ53_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43400.1"
                     /translation="MVGLGLLMKGHLYTWSDMDRLSQLYFVSDIGIGLVYPVLYIFNV
                     GRELLPGLPLFEHANNFLAVAGLLNYLIIFDAFDIGMGRKA"
BASE COUNT          418 a          398 c          377 g          330 t
ORIGIN      
        1 acccaatcgc agcggccacg cctgaatccg ggattgggac cacaatatca gccgggacgg
       61 gatgctcaag ggccagccgt tttcccattt tatggcgact tttgttcaca ctccggccaa
      121 aaaccaggct gtctggtcgt gaaaaataca catgctcaaa aatacaaaat gccggtttgt
      181 gttctggaaa cggcgattgg gcgtgaatac catcccgatt gatcatgacg acttcgccgg
      241 gttgcacgtc tcgcaggtag gtggcaccaa tcaaatcaaa ggcgcaggtt tcagacgcca
      301 ccacatatga atgttcatcg agttgaccaa tgcacattgg acggaagcca tgcacgtcgc
      361 ggacggcgat caggcaatcg gtggtttgaa tcagaatcga ataggcgcct tcaatcgagc
      421 gaaaggtttc aacgacggct tcatacagtg aggctttgcg tgagcgggca agcttgtgca
      481 acacgacttc ggtatcgctg gttgatgaaa aaatggcgcc ttcggcttcg agttcgcgcc
      541 gcatctcact ggcaaaagga aggtttccat tgtggcaggt cgcaatctca ccccagtgac
      601 acttgatcgc aaacggctgg gcttcattga tactgacttc tccggcagtt gagtaccgga
      661 catggccaat cgcccggttg ccaggcaatc gccgaaaggc ttcctcggtg aaaatttcgc
      721 ttacgtgacc catgccgcgt tcactataca gccggttccc atcgcttgag acaatcccgg
      781 ctgattcctg accccgatgt tgcagggagt acagaccaag ataggttaat cgggcggctt
      841 cggggtgtcc ccaaatacca aaaacaccac attcttcgcg aaatttatcg tgcatatcag
      901 aattcaagaa gttacagggg catcgttcaa acgagatcaa cgcgggagtc acttacagta
      961 aatacatcag ccagctcagt gccaggccaa ttccaataaa tgacagaaaa acccgcaatc
     1021 catagaggaa gcgtgctttg ggtgtgctga cttcgcttga aacacctgca aacacggcag
     1081 agacaaaagc tgcaaaaagt accattaaga ggaagtgcat agtattacga tgtcaggaaa
     1141 ctggacctct gaaggaatgc aagatgacgg gcgagagcct gctcaattct tcggtccgat
     1201 gtcttcaccc ggtgttgttg gccctgggcg tgtgaatttt cagccctcaa ccgcaggccc
     1261 tggctcaggc tttgcgtccc ataccaatat cgaaggcatc aaaaataatc agataattga
     1321 gcaatcccgc caccgccaaa aaattgttgg catgttcaaa caatggcagg cccggcagaa
     1381 gttcccggcc aacattaaaa atgtacaaaa ccgggtatac caggccaatc ccaatgtcac
     1441 tgacaaaata taattgtgac aaccggtcca tatccgacca ggtatagaga tgtcctttca
     1501 tcaagagtcc cagtccaacc atg
//
LOCUS       DAOQDO010001842         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94374_length_1523_cov_2.569482, whole genome shotgun sequence.
ACCESSION   DAOQDO010001842 DAOQDO010000000
VERSION     DAOQDO010001842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94374_length_1523_cov_2.569482"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKZ53_23140"
     CDS             <1..745
                     /locus_tag="PKZ53_23140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNJ43401.1"
                     /translation="VLLAGSGWAEGLKWFFFGFTCVFLPLMAYQSRKGFQHLDLDALR
                     HFSRVGNYLSSIVILLILLGLSLGTAWSHQLELFSGPVFTVRALVTLMALLSGALTLL
                     RIFLPVAAQEPVIQYIIPTTNIEFVWFSALSITAGLAEEIAFRGVLTSLLQQAWGSYW
                     LATLASALVFGLAHLLQGVRGVVVAACFGLVNQMGTSWTGTLLVAIIVHALYDIVAGY
                     WLAQMNQELRMKNEEVSTTCVSGWVKNEE"
     gene            complement(965..1090)
                     /locus_tag="PKZ53_23145"
     CDS             complement(965..1090)
                     /locus_tag="PKZ53_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43402.1"
                     /translation="MKNEEVSTTAMTKLTSTLSFMSFMSFMSFDLGSINHNERQY"
     gene            1191..>1523
                     /locus_tag="PKZ53_23150"
     CDS             1191..>1523
                     /locus_tag="PKZ53_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43403.1"
                     /translation="MKAFHLSDEFIDADAERQMIAAVARRPDLFWELGELLRPDVFSA
                     ENATWARLVYEIEERAPITQGQAWEPARNPQATAQHLADLFQRRLLAGTQEQLAAGLF
                     AHAPVSELV"
BASE COUNT          329 a          384 c          383 g          427 t
ORIGIN      
        1 ggttttgctg gcgggttcag gctgggccga agggctcaaa tggttctttt ttggctttac
       61 ctgcgtgttt cttcctttaa tggcctatca aagccggaaa ggatttcaac accttgacct
      121 ggacgccctg cgccactttt ccagagtggg aaattacctc agctccattg ttatcttact
      181 cattttgctc gggctttcgc ttggtacagc ctggtcacat cagttggagt tgttttcagg
      241 acccgtgttt accgtcagag ccctggtaac cttgatggcc ctgctcagtg gggccttgac
      301 cttgttgagg atttttttac cagttgcggc gcaagagccg gtcattcaat acattattcc
      361 aaccaccaac attgaattcg tgtggtttag cgccctgtca atcacggcgg gactggccga
      421 agaaattgca tttcgcggcg tactgacctc acttttgcag caagcctggg gttcgtactg
      481 gctggcaacg ctggccagcg ccctggtatt tgggctggct catttgctcc agggcgttcg
      541 tggtgtcgtc gtggcagcct gtttcggact ggtcaaccag atgggtacca gttggacggg
      601 aacgctgctg gtagccatca tcgtgcatgc cctctatgac atcgtggccg gctactggct
      661 ggcgcagatg aatcaagaat tgagaatgaa gaatgaagaa gtcagtacca cctgtgtcag
      721 cgggtgggtg aagaatgaag aatgaagaaa attcctggcg actggttttt ctttgaaagg
      781 agtgatttcc aaccactagc tgtaataaca gacactattg tttcaatggt tttgagcgtt
      841 cacctgattt ttcaaaggac tacctcggca acatctgaaa caacatgaga tgtcattaca
      901 acaaaccatg agcaactccg ctttctttac tccctcaatt tcacccaaaa cactgaccac
      961 tttctcagta ttggcgttca ttgtggttga ttgagcccaa atcaaaggac ataaaagaca
     1021 taaaggacat aaaggataag gtggaagtca gtttggtcat cgcggtggta ctgacttctt
     1081 cattcttcat tcttcattct tcattctaac cgcttctcat tttttcgatt ccggagtatt
     1141 tttgtcattc ctgaccaggg tttccactct ttttttttcg catccagccc atgaaagctt
     1201 ttcacctgag cgacgagttt atcgacgccg acgccgaacg tcagatgatt gctgcggttg
     1261 cacgccgtcc cgatctgttt tgggaattgg gtgaactgtt gcggcctgat gtcttttcgg
     1321 ctgagaacgc gacctgggcg cggctggtct atgaaattga ggaacgggcg cccattactc
     1381 aagggcaagc ctgggaacca gcccgcaacc cgcaagccac tgctcagcat ctggccgatc
     1441 tatttcaacg gcggctcctg gctggcacac aggaacaact tgctgccggg ctctttgccc
     1501 atgctcccgt ctcagaactg gtc
//
LOCUS       DAOQDO010001843         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94377_length_1523_cov_2.530654, whole genome shotgun sequence.
ACCESSION   DAOQDO010001843 DAOQDO010000000
VERSION     DAOQDO010001843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94377_length_1523_cov_2.530654"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..>1523)
                     /locus_tag="PKZ53_23155"
     CDS             complement(110..>1523)
                     /locus_tag="PKZ53_23155"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43404.1"
                     /translation="REQLNRKADEQLQLLSRNAPTDQQESVKREIQKLTDEYEMVCSQ
                     IRLQNPRYVSLTNPGTLTLAQIQQQLDASTVLLEYALGEKRSYVWMVTQSGISVQELP
                     READLSRLGQELYQSISNRAISDSKKKPAGAPDYQSPSRRLGQLLLGPFASQLTGKRL
                     VIVSDGALQYLPFSVLSLATPGAKGKPAWVPLIAEHEIVHLPSVATLKVLRQEIEGRR
                     PAPKSLAILADPVFSPTDERLLAHPPTTTTRLTTAERDLKLNSLNQSTSDVRLGALGL
                     EIPRLPYSRLEAEAIAKLVPPSERIQALDFEASRKLALSATLGQYRILHFATHSLLNT
                     VHPQLSGIVLSLYDRSGVAQDGFLRVNEIYNLKLPAELVVLSACNTGLGKDVRGEGLI
                     GMTRGFMYAGAPRLITSQWRVDDQATAELMTTFYDGMLGKTQLTPASALRTAQVKMWR
                     HAKWNHPYYWAAFELQGEWK"
BASE COUNT          340 a          398 c          410 g          375 t
ORIGIN      
        1 ctgtcaggac aaggctttcc gccttgcctc tcccagaatc tccagatttt tcaaaggttt
       61 acgtcccttc ttcaaaggct gcgtaaaccc tatcttcatt cttcattctt catttccact
      121 cgccttgcag ctcaaaggcg gcccaatagt acggatgatt ccatttggcg tgccgccaca
      181 tttttacctg ggcggtacgc agcgcactgg ctggggtcaa ttgtgttttg ccaagcatgc
      241 catcataaaa tgtcgtcatc aattcggcag tcgcctgatc gtcaacccgc cactgactgg
      301 taatcaaccg tggcgcaccg gcatacataa aaccgcgtgt catcccaatc aaaccttccc
      361 cacggacatc tttcccaagc ccggtattgc aggcactgag caccaccagt tctgccggga
      421 gtttgaggtt gtaaatctca tttacccgca agaatccatc ttgcgcgacg ccactccggt
      481 cataaagcga aagaacgatc ccggaaagtt gcggatgcac cgtattgagc aagctgtggg
      541 tggcaaaatg gagaatccga tattgcccaa gcgtcgcact gagagccagt tttcgactgg
      601 cttcaaaatc aagcgcctgg atgcgctctg acggtggcac cagtttggca atggcttcgg
      661 cttccaggcg cgaataaggc aggcgcggaa tttcgaggcc cagtgcacca agtcggacgt
      721 ctgaggttga ttgattgagt gaattgagtt tgaggtcacg ttcggcagtc gtcagacgag
      781 tggttgtggt tggaggatga gccagcaggc gttcatcagt tggtgaaaac accgggtcgg
      841 ccaaaatggc cagcgatttt ggggccggac ggcggccttc aatttcctgg cgcaacacct
      901 tgagggtggc aaccgacggc aaatgcacga tttcatgctc ggcgatgagc ggtacccagg
      961 ctggtttgcc ttttgctcca ggcgtagcaa gtgacaacac cgaaaatgga aggtactgga
     1021 gcgctccatc cgaaacaatc accagccgct tgccagtgag ttgactggca aatggcccca
     1081 gcaaaagctg tccaagtcgg cgtgatggcg actgatagtc tggagcgccg gcgggtttct
     1141 ttttggagtc actgatagcc cggttggaaa tagattgata cagctcctgg cccagccggc
     1201 tcaggtcagc ttcacgcggc aattcctgaa cgctgattcc agattgggtg accatccaga
     1261 cataacttcg cttttctcca agcgcatatt ccagcaagac agtgctggca tccagttgtt
     1321 gctgaatttg cgcaagcgtt aaggttcctg ggttggtaag tgaaacatac cttgggtttt
     1381 ggagtcgaat ttgactacaa accatttcat attcatcggt cagcttttga atctcacgtt
     1441 tgaccgattc ctgctggtca gtgggggcat ttcggctcag gagctggagt tgttcatcag
     1501 ctttgcggtt gagttgctcg cga
//
LOCUS       DAOQDO010001844         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94458_length_1522_cov_3.799591, whole genome shotgun sequence.
ACCESSION   DAOQDO010001844 DAOQDO010000000
VERSION     DAOQDO010001844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94458_length_1522_cov_3.799591"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..>1522
                     /locus_tag="PKZ53_23160"
     CDS             329..>1522
                     /locus_tag="PKZ53_23160"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43405.1"
                     /translation="MPAPNRWLFFSRLFVVCGLFLFLSLILRPILSASTPATVVSKSV
                     SQPDEPSLRGEAAIKHLQEQGTYQSLQEAVNRAQYDVLWRDQTPVKGLASGAFEAINQ
                     KQKFATWFTDDRIVVHPSHAGVPDWEFQFKFESLGYGATQYPVGPGKVSAELSKVENA
                     RLVGGITKLTEWYINRSEGLEQGITIEAPPAQRVPGERMRLGFRVGGDLFPQLEDPSA
                     INFTRRNGSGTALRYDQLYVTDSIGNAVPAQFAVEGGKVWIEVDDAQAVYPINIDPTF
                     ALPFKLVGPTPLGAGGSVAVYGNTAVVGTISNRVFVYVQDMAGAWTLQATLTPSDTST
                     PADFGIDVTIYGNTIAVGAFLQGPGRVYIFNRTGTTWSETQILTNPDLEPGAWFGYSV
                     AMNVDT"
BASE COUNT          325 a          407 c          393 g          397 t
ORIGIN      
        1 gcacccagtt caaccgtaat cacgtgaaat gtcatttcat caatcattgg cgctgatttc
       61 cgacaaatca ttcccgcttg tcacaaaata agtcccattc gtcatgtgta cgttgagttt
      121 ggcgcagatt ctgcgctagg ttcatcagtc ttttttcaag ttgtctctcg ttgccgcact
      181 ttcttttcca cgtcatccat ttcaaaaact ccaggccagg ggcaaagcat gcccgccatg
      241 ctcgaacgat tggtgtttct atctgggagc accgaagttt gtcgccttca atctgacttg
      301 atggccagct ttccagggtg gagtttttat gccagcacca aatcggtggt tgtttttctc
      361 gcgtttgttc gtcgtttgtg ggttgtttct gttcctgagt ttgatcctga gacccattct
      421 gtccgcttcg acacctgcca ctgtggtttc aaaatctgtc agtcaaccgg atgaaccgtc
      481 attgcggggc gaggcggcca tcaagcacct tcaggaacag ggcacgtacc agtcactcca
      541 ggaagccgtc aaccgggccc agtacgacgt tctctggcga gatcaaacac ctgtcaaagg
      601 gctggcttcg ggtgcgtttg aagctatcaa ccagaagcaa aaatttgcca cctggtttac
      661 tgatgaccgg attgtggtgc atccatccca cgctggtgtg cctgactggg agtttcagtt
      721 caagttcgaa tcactcggct atggagccac ccagtatccc gttggcccag ggaaggtttc
      781 ggctgaactt tcgaaagttg aaaatgcccg gttggttggc ggaatcacca aactcaccga
      841 atggtacatc aaccgttccg aaggactgga gcagggcatt accatcgaag ccccacccgc
      901 tcaacgcgtt ccaggtgaac ggatgcgcct tggctttcgc gttggcggag acctctttcc
      961 ccaactcgaa gacccctctg ccatcaattt tacccgccgc aatggctctg gcacggcgct
     1021 cagatatgat cagttgtatg tgacggattc aattggaaat gctgtaccgg cacagtttgc
     1081 ggttgaaggc ggaaaagtct ggattgaagt tgatgatgcc caggcggtgt atccgatcaa
     1141 cattgaccca acctttgccc ttccgtttaa actggtcgga ccaactcctc ttggagctgg
     1201 cggatccgtc gccgtctatg gcaacacggc tgtggttggg accatcagca accgggtctt
     1261 tgtctatgtg caggatatgg ccggggcctg gacgctccag gcgacgctca ccccatctga
     1321 caccagtact cccgctgatt ttggaattga tgtcacgatt tatgggaaca cgattgccgt
     1381 cggtgctttt ttacaggggc caggacgggt ctatatcttt aatcgaacgg gcaccacgtg
     1441 gagtgaaacc caaatcttga cgaatccaga tttggagcct ggcgcctggt ttggctattc
     1501 ggtggccatg aacgtagaca ca
//
LOCUS       DAOQDO010001845         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94799_length_1520_cov_2.019113, whole genome shotgun sequence.
ACCESSION   DAOQDO010001845 DAOQDO010000000
VERSION     DAOQDO010001845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94799_length_1520_cov_2.019113"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1520
                     /locus_tag="PKZ53_23165"
     CDS             94..>1520
                     /locus_tag="PKZ53_23165"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ43406.1"
                     /translation="MQGIFQWRVAMGFENVQFTKLKYTFGVGLGWMLMGLLLPAVCLA
                     QDGSGYIRGKVLDADQKAIPGATVVVKSLRSGQELTTQTDLSGAFTIQNAEIGTYEVR
                     VEATGFELHQSEYEVLPGTGQPALEITLGIEGGSFESTTVANISATFRTLTPDVQKAD
                     IKTQIQTKQVVELPTITRNIYGLTALSGNITATTPPNATSTDATLINPDVRGSGFNIN
                     GLRSASTVITLDGGLNVNPFTSTIGQNVPLDAVNELNITTNNPSAGVGRASAGLVAVT
                     TKSGTNEFHGTVYEFNRNSALASNGFDNNARGIDKGYFQRNQFGYSFGGPIRKQKAWF
                     FSSAEGLTVNSREDRLFVVPAQSFLTGPGVNKATQDFFSQYGQLVPGQAQSSILLGQK
                     TAPNPLPGGLFDVFRATDLSVDAGGGAPQSSIQLVNRVDWNLSNDTRITARHAFERID
                     RDPGSFSVSPYTGFDTGSLETNHSLL"
BASE COUNT          386 a          397 c          386 g          351 t
ORIGIN      
        1 gctgactgcc gacgacaaac tggtatgaga caaccgggcc cattcaaacc aaacaatttg
       61 atttttcata ccaacgtgga gcgagaatta cttatgcaag gcatttttca atggcgtgtc
      121 gcgatgggtt ttgagaacgt tcagttcaca aaattaaaat acacctttgg ggttggattg
      181 ggttggatgt tgatgggtct tctccttcca gccgtttgtc tggcccagga tgggtctggg
      241 tacattcgag gaaaagtcct ggacgcagat caaaaggcaa ttcctggcgc cacggtggtg
      301 gtcaaatcgc ttcgttcagg tcaggaactg accacccaaa ccgatctcag cggggcattt
      361 accattcaga acgcagagat cggaacctat gaggtgagag ttgaggcaac cgggtttgaa
      421 ctgcatcagt cggagtatga agtcctgcct ggaaccgggc aacctgcgct tgaaatcacc
      481 cttggcattg aaggcgggag ttttgagtca acgacggtgg ccaacatttc ggcaacgttc
      541 cgaacgttga cgccagatgt tcagaaagct gatatcaaaa cccaaattca aaccaaacag
      601 gtggttgaat taccaaccat cacccgaaac atctatgggc tcacggcgct ctctggcaac
      661 atcaccgcca ccactccccc aaatgccacc agtactgatg ctacactgat aaaccccgat
      721 gtacgtggaa gtggttttaa tatcaatggg ttacggtctg ccagcaccgt gatcaccctc
      781 gatggcgggc tcaatgtcaa cccatttacc tcaaccattg ggcagaatgt gccgctcgat
      841 gctgtcaatg aactcaatat caccaccaat aacccatcgg caggtgttgg acgagccagt
      901 gccggtctgg tggccgtgac aaccaaatcg ggcaccaacg aatttcacgg cacggtgtat
      961 gaattcaacc gaaactcagc gctggcttcc aatgggtttg acaacaatgc gcgcggcatt
     1021 gacaaggggt attttcagcg gaatcaattt ggatattcgt tcggtggacc gattcggaag
     1081 caaaaagcct ggttctttag cagcgcggaa ggtctgacgg tgaacagtcg ggaagaccgc
     1141 ctctttgtgg tacccgctca atcctttctg actggtccag gtgtcaataa ggcaacacaa
     1201 gattttttca gccagtatgg tcagttggtc cccgggcaag ctcagagttc aatcctgctt
     1261 gggcaaaaaa cagcccccaa tccactcccc ggcggactct ttgatgtgtt tcgggcaacc
     1321 gatctttcag ttgatgctgg tggaggtgca ccacaaagct cgattcagct tgtcaatcgg
     1381 gttgattgga acctcagtaa tgacacccgg attaccgccc gtcacgcctt cgagcgcata
     1441 gatcgagacc cgggaagctt ctcagtcagc ccctataccg gatttgatac tggcagcctc
     1501 gaaaccaatc acagcctgct
//
LOCUS       DAOQDO010001846         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94884_length_1519_cov_2.474044, whole genome shotgun sequence.
ACCESSION   DAOQDO010001846 DAOQDO010000000
VERSION     DAOQDO010001846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94884_length_1519_cov_2.474044"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKZ53_23170"
     CDS             <1..1359
                     /locus_tag="PKZ53_23170"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNJ43407.1"
                     /translation="KGEDTRLSITGRIGAAGKTTLGLDGDVNLKFLRNVTKNTLYTSG
                     VARLKAAVSGTLDDPRITGTADIDRFAMQVKDFPLILENGGGRVLFTNNQAQIDTFTA
                     DAGGGKVELSGGAVIDTLKPVGLRWRFGLRADNVRMNYPQDVRSLLDGDLVYQGNLKL
                     QILQGTIKVKRAEYTERTDLVSLLQSTLAATASNSSDLQATVSPQSQLRFDVRLEAPE
                     SLFVRNNLADMVGTASLRLSGSSTDPRLIGRVVISRGQLELRNDRYQITRGIVVFPEA
                     RSEKVFFDIQAETELQNYRVIVGATGNPDKFSSILRSEPALPQSSIVTLLATGNLPPS
                     GQSQNDPYTTTQTGVSAASSLVAELFSERVEERTNKIFGINRFQIDPLLVGRGNDPTA
                     RVTIGRRITKDLSITYSTNLATTQEQIILVEYRLSPTVSVIGIRDQRGNFGVDVRFTK
                     RF"
BASE COUNT          379 a          404 c          376 g          360 t
ORIGIN      
        1 aagggagaag acacccgact ttcaataacc gggcgcattg gggccgccgg caaaacaact
       61 ctggggctgg atggcgacgt aaacctcaag tttttgcgca atgtaacgaa aaacacgctc
      121 tataccagcg gtgtggcccg actcaaagcc gcggtttcgg gcaccctcga tgatccgcgg
      181 attaccggaa cggctgatat tgaccgcttt gccatgcagg tcaaggattt cccattgatc
      241 cttgaaaatg gtggcggacg cgttctattt accaataacc aggcccagat tgataccttt
      301 acggcagatg ccggtggtgg aaaagtcgaa ctctcaggtg gagcggtcat tgataccctc
      361 aagccagttg ggttgcgctg gcggtttggc cttcgggctg acaatgtgcg gatgaactat
      421 ccacaggatg tgcgctcgct gctggatgga gatctggtgt accagggaaa tctgaaactg
      481 cagattctgc aaggaaccat caaagtcaaa cgtgctgaat acaccgaacg aaccgatctg
      541 gtgtcgttgc ttcaatccac ccttgccgcc actgcctcaa atagtagcga cttgcaggca
      601 actgtttcac cccaatctca acttcggttt gatgtgcgac ttgaggcgcc agaatcgctg
      661 tttgtccgca ataatctggc agatatggtt ggaaccgcat cgctccggtt gagcgggtcc
      721 agcacggatc cgcgactgat cgggcgagtc gtcatcagcc gtggtcagct tgagttacgc
      781 aatgaccggt atcaaatcac acgcggaatt gtcgtctttc ctgaagcccg ctcagaaaag
      841 gtattttttg atattcaggc cgaaaccgag cttcagaatt atcgggtcat tgtgggggca
      901 acaggcaatc ccgacaaatt ctcatctatt ttgcgctcag aaccagcgct cccccaaagc
      961 agcattgtga ccttactcgc caccgggaac ctgccgccat ctggtcaatc tcaaaatgac
     1021 ccctatacca caacccaaac cggagtgagt gccgcctcca gcctggtggc tgaactgttt
     1081 tcagaacggg ttgaggaacg caccaacaag attttcggaa tcaatcgttt tcaaattgac
     1141 ccactgctgg tcggtcgtgg caatgacccg accgcacgcg taacaattgg ccgacgcatc
     1201 accaaagatc tttcaatcac ctattcaacc aacctggcca ccacccagga acagatcatc
     1261 ctggttgaat atcggctttc accgaccgtt tccgtcatcg gaattcgcga ccaacgcgga
     1321 aatttcgggg tggacgtgcg gtttaccaaa cggttttaaa aggaatgaag aatgaagaat
     1381 tgagaatgaa gaatatgctt ggtgcttggt ttctggtgcc tggttcttgg ttcttcaaaa
     1441 gtattgattc ctaagcacca agcaccaagc accaagcacc aaccactttt tgaattcttc
     1501 attcttcatt cttcattct
//
LOCUS       DAOQDO010001847         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94973_length_1518_cov_2.920711, whole genome shotgun sequence.
ACCESSION   DAOQDO010001847 DAOQDO010000000
VERSION     DAOQDO010001847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94973_length_1518_cov_2.920711"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKZ53_23175"
     CDS             <1..395
                     /locus_tag="PKZ53_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43408.1"
                     /translation="VSYLSDKQNGQVKLINEINELAAKNQVRLTDGIEFESAKVELPP
                     VEGQKTRRLTASDQTNEQLAYPGIVLRFYVVGPYYNMRRFIKAVEASKQFMLIQLGEL
                     SSENVKEGGGADIVQVSFQITAFFQRGD"
     gene            476..1057
                     /locus_tag="PKZ53_23180"
     CDS             476..1057
                     /locus_tag="PKZ53_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HNJ43409.1"
                     /translation="MKLIPSRKACEVLGIHPNTLRKWADNGTIQHIRTKAGQRLYDVE
                     SITGQGTGRKICYCRVSSAKQRDDLGRQIKSLRDAFPNHEIISDIGSGLNFKRKGLTA
                     LLEAVCRGDVREVVVAHRDRLARFGFDLIKWLIEHHGGKLVVLGTDNCSPESELVHDL
                     LAIVNVFSGRMPGLRKYKNQIKSQIKEDRDLSG"
     gene            1119..>1518
                     /locus_tag="PKZ53_23185"
     CDS             1119..>1518
                     /locus_tag="PKZ53_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ43410.1"
                     /translation="MEYLKQPGTKANWKAIKTEIIRKLPEWAKNTPYQIKSIAIRDAC
                     NAVKAAKKKFIQTKQFQEVKFRSRKKRLDSIFIPSSAVSSASVYPNLLGSSLRPSEPF
                     PESVIHDCRLVIDHGRYFLAVSIDVTPIQPE"
BASE COUNT          441 a          362 c          358 g          357 t
ORIGIN      
        1 aagtgagcta tctgtcagac aaacagaatg gtcaggtcaa acttatcaac gaaatcaatg
       61 aactggcggc caaaaatcag gttcgcctga cagatggcat agaatttgag tctgcaaagg
      121 tggaactccc accggttgaa ggacaaaaaa cacggcgatt gactgccagc gaccagacca
      181 acgagcaact tgcgtaccct ggaattgtgc tcagatttta cgtggttggt ccttattata
      241 atatgcgtcg gtttatcaaa gccgtcgaag ccagtaagca gtttatgtta atccaactgg
      301 gcgaattgag ttcggaaaat gtcaaagagg gcggaggggc tgatatcgtc caggtaagtt
      361 ttcaaatcac cgcgtttttc cagcgtgggg actgatctga tcgtagccat agagattgac
      421 aaatagcagc gatattatta atttttgtta atcttagtta acaattgcca ggcttatgaa
      481 acttatccct agtcgcaagg catgtgaagt tcttggcatt caccccaata ctctccgaaa
      541 gtgggcagac aatggaacaa ttcaacatat ccgaaccaaa gccgggcagc ggctctatga
      601 tgttgaatcc attaccggac aaggcactgg ccggaaaatc tgctactgcc gggtctccag
      661 cgccaaacag agagatgacc ttggtcggca aatcaaatcc cttcgcgatg cgttccctaa
      721 ccacgaaatc atttcagaca ttggaagcgg acttaacttc aaacgtaaag gacttacagc
      781 cttactggaa gccgtgtgtc gcggagatgt ccgggaagtt gtggttgccc atcgggaccg
      841 actcgctcga tttggatttg accttatcaa gtggcttatc gagcaccacg gtggaaaact
      901 cgtggttctc ggcacagata attgctcccc agaatccgaa ctggttcacg atctactcgc
      961 catcgtcaac gtgtttagtg gccgaatgcc cggactccga aaatacaaaa atcaaatcaa
     1021 atctcaaatc aaagaagatc gagatttatc cggataagga acaagccgca cttcttcgcc
     1081 ggtgggcggg aacggcccgg tatgtattca accagaccgt ggaatacctg aagcaaccag
     1141 gtacaaaagc caactggaaa gcgatcaaaa ctgaaattat ccgtaaattg ccggagtggg
     1201 cgaaaaatac gccatatcag attaagtcca ttgcgattcg ggacgcctgc aacgcggtaa
     1261 aggcggccaa gaaaaaattc attcaaacca aacagttcca ggaagtaaaa ttccgttctc
     1321 ggaaaaagcg attggattca atttttatcc catcatcggc agtttcaagt gcttcggtct
     1381 accccaactt acttgggagt tccctgcgac cttccgagcc tttccctgaa agcgtaatcc
     1441 acgattgccg gctggtaatt gaccacgggc ggtactttct cgctgtatca attgacgtaa
     1501 ctccgattca acctgaga
//
LOCUS       DAOQDO010001848         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94980_length_1518_cov_2.784005, whole genome shotgun sequence.
ACCESSION   DAOQDO010001848 DAOQDO010000000
VERSION     DAOQDO010001848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94980_length_1518_cov_2.784005"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1518)
                     /locus_tag="PKZ53_23190"
     CDS             complement(71..>1518)
                     /locus_tag="PKZ53_23190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43411.1"
                     /translation="GSNLRSVIELRGNSHALGPVFIFNNVFQNNQPELDGALFYWVRE
                     NGQPSTFFHANNLFLNNIAVTDTPITGESGSLYTVDPMLTAPVVPSLTNLTFERLEQI
                     RAGFALKPGSPAIDRGLNAIARTGHPNWKPGVTDRRWDFQQQVRPETNTWDLGVDEVI
                     AADTTPPTVRVIAPNGGETISSGSQLTISWASSDAVGVVSQAISLSTDGGTSFPTSVT
                     TGLAGNVTEFQFAVPVSMTTTAGRIQVIARDGAGNTGLDASDANFTIRPASDSLAPTV
                     KVLAPNGGEIVESGSPLMISWSSTDNVGVVSQGINLSTDGGNTFPTSVATGLSGMTSE
                     FQYSVPDTISTDLAQIQVTARDLAGNAGTDTSDASFTIRSVGDTTPPAVRVIAPNGGE
                     KLKGRASATVSWESSDDTALARHDVLLSTNGGASFTTELATGLAGNLQSFTFTVPATK
                     TKTGRIRVVAVDTSGNQSQDESDGNFKIKVK"
BASE COUNT          359 a          406 c          396 g          357 t
ORIGIN      
        1 gcaccaggca ccaggcacca ggcaccaggc accaggcacc aggcaccagg caccaaacac
       61 caggcacccc ttacttcacc ttgatcttga aattcccgtc actttcgtcc tgagactggt
      121 tgccgcttgt gtcaaccgca accactcgga tccgcccagt tttggttttg gttgctggaa
      181 cggtaaaagt aaaggattga agatttccgg ccaatccggt ggccagttca gttgtaaaac
      241 ttgcccctcc attggtcgaa agcaacacat cgtgccgtgc aagcgctgta tcatccgaag
      301 attcccaact cacagtggcg ctggcacgac ctttgagctt ttcgccgccg tttggcgcaa
      361 tcactctgac cgctggtggt gtggtgtctc caactgaacg aatcgtaaaa ctggcatctg
      421 aggtgtctgt gcctgcattt ccggccagat cacgggcagt gacttgaatc tgggctaaat
      481 cggttgaaat ggtatctggc actgagtact gaaattcact ggtcattccc gaaagcccgg
      541 tggcaactga agttggaaat gtgtttccgc catcggttga aaggttaatt ccctgtgaaa
      601 ccacaccgac gttgtcggtt gatgaccacg aaatcatcaa tgggctaccg ctttcgacga
      661 tttcaccgcc gtttggagca agaactttca ctgttggcgc caggctatct gacgccgggc
      721 gaatggtgaa attcgcatcc gatgcgtcca gccctgtatt tcctgccccg tcacgagcaa
      781 taacctgaat acgaccagca gttgtagtca ttgagacggg aacggcaaac tggaattcag
      841 tcacattgcc ggcaagtcca gttgtgactg aagttgggaa tgaagtgcct ccgtctgtcg
      901 aaaggctgat tgcctgtgaa accactccaa cggcatcgct tgaagcccag gaaatggtca
      961 gttgacttcc gctggagatg gtttcgccgc cattgggtgc gatcactctg acggtgggcg
     1021 gcgtggtatc agcggcaatc acttcgtcaa cacccaaatc ccaggtattt gtttcaggac
     1081 gcacttgttg ctggaaatcc cagcgtcggt ctgtcacgcc aggcttccag tttggatgac
     1141 cagttcgagc aatggcattc aatccacgat caatggccgg tgaaccaggt ttgagcgcga
     1201 atccagcccg aatttgctca agtcgttcaa acgtcaggtt cgtcaacgat ggaactaccg
     1261 gggcggtcaa catcggatca accgtgtaga gactcccgga ttcaccggta attggagtgt
     1321 cagtcaccgc tatgttattg agaaatagat tgttggcgtg aaaaaatgtc gaaggctgcc
     1381 cattttcgcg cacccagtag aaaagcgcgc catcaagttc cggctgattg ttctgaaaca
     1441 cgttgttgaa aatgaagact ggccccagcg catgcgaatt gccacgaagt tcaatcactg
     1501 accgcaggtt tgaaccgc
//
LOCUS       DAOQDO010001849         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_94985_length_1518_cov_2.574846, whole genome shotgun sequence.
ACCESSION   DAOQDO010001849 DAOQDO010000000
VERSION     DAOQDO010001849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94985_length_1518_cov_2.574846"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1518)
                     /locus_tag="PKZ53_23195"
     CDS             complement(119..>1518)
                     /locus_tag="PKZ53_23195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNJ43412.1"
                     /translation="LRMVPVGSLTARLHRTVRVTAEHQAKLAKLTVEGEQVELDKTVL
                     EEIADPLLHILRNAVDHGIEPPALRQALGKPKIGHIRVRATYEATQVVIQVSDDGGGI
                     EPDVVRAAAVRRGHISETEAAQLTDDEIYSFLFLPGFSTASEVSEISGRGVGMDIVKE
                     TVTRMKGTVAIQSVPGQGMTLTIRLPMTLAILRVILVKASTETFAIPLAPVTRILRIE
                     KEDIEYIGQKPVIRVEGEVYPLLHLGTVLNLKHPADETLRRVPVLILNLGSQQIAVAV
                     DQLVEAREVVVKSMGNLLQRVPGIAGATLMGDGSVVLILNPNELVRDTTRSEAQPRSV
                     VRTPAKVREVLQVLIVDDSVSVRRVLSNFVRNQGWTPTEARDGLEALELLQSGTVTPD
                     IVLLDIEMPRMDGYELTTTVRSNAALKHIPIVMLTSRAGEKHRRKAMDVGATDYLVKP
                     YQEEVLLGVMRRLTQ"
BASE COUNT          306 a          429 c          404 g          379 t
ORIGIN      
        1 cagggttcgg ggttcggggt ttcaaattga ggtcctttgt ccttttcgtc cgtttggtca
       61 cttatgtcct tcaggtcttt tttgcctttt cctcaaaccc tgaaccctga accctcattt
      121 actgagtcaa tcgtcgcatc actcccagca gtacttcttc ctgatagggt ttgaccagat
      181 agtcggtagc accaacgtcc atggccttcc ggcggtgttt ttctccggca cgagatgtga
      241 gcatcacaat cggaatgtgc tttaaggctg catttgaccg aaccgtggtt gtgagttcat
      301 agccatccat gcggggcatt tcaatgtcga ggagcacaat atctggagtg accgttccgc
      361 tctgcagcag ttcaagggct tcgagtccat ctcgcgcttc agtcggagtc cacccctggt
      421 ttcgcacaaa attggaaagc acccggcgca cactcactga atcatccacg atcaaaacct
      481 ggagcacctc acggactttg gctggtgtcc tgacaactga tctgggctgg gcttctgacc
      541 tggtagtatc tcggaccagt tcgtttggat tgagaatgag aaccacactg ccgtctccca
      601 tcagggtggc gccggcgatg ccggggacac gctgtaagag attgcccata gatttgacca
      661 ccacttcgcg agcttccacc aactggtcca cagccaccgc aatttgctgg gacccaaggt
      721 tgagaatcag caccggaacc cgacgaaggg tctcgtcggc tgggtgtttg agattgagca
      781 ctgttcccag gtgcaggagc ggatacacct cgccttcaac tcgaatgacc ggcttttgcc
      841 cgatgtattc gatgtcctct ttttcgatcc gcaaaattcg cgttaccggg gccagcggaa
      901 tggcaaatgt ctcggtgctg gctttgacca gaatgacccg cagaatggcc agcgtcatgg
      961 gcaggcggat agtcagggtc atcccttgac ccggaacgga ctggatcgcc accgtgcctt
     1021 tcatccgggt cacagtttct ttgacaatgt ccatgccgac gccacgacca gaaatttcac
     1081 tgacttctga tgctgtgcta aatccaggta gaaagaggaa ggaataaatt tcgtcatccg
     1141 tcaattgggc ggcttcggtc tcggaaatat ggcctcgccg aaccgccgct gcccggacca
     1201 cgtccggttc aattccgccc ccgtcatcac tgacctggat taccacctgg gtcgcttcat
     1261 aggttgctcg tacccggata tgtccgattt ttggttttcc aagggcctgg cgcagggccg
     1321 gaggttcgat tccgtggtca actgcattgc gcaggatgtg caagagcgga tcggcgattt
     1381 cttcaaggac ggttttgtcg agttcgacct gttcgccttc aacggttaat ttcgccagct
     1441 tggcctgatg ttcggcagtg actcgaactg tgcgatggag ccgcgccgtc aacgacccga
     1501 ctggcaccat ccgtaacc
//
LOCUS       DAOQDO010001850         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95078_length_1517_cov_3.435705, whole genome shotgun sequence.
ACCESSION   DAOQDO010001850 DAOQDO010000000
VERSION     DAOQDO010001850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95078_length_1517_cov_3.435705"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..>1517
                     /locus_tag="PKZ53_23200"
     CDS             126..>1517
                     /locus_tag="PKZ53_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43413.1"
                     /translation="MLNIRRVILYKHGVGYFERVGKVVDDQRVDLFFRASEVSDVLKS
                     LTVIDLDGGTVASVSYDSIKPIEELLADIAFNIPDEGSLLRLLPQLKGAEVEVQVGSE
                     TIVGTVIGIDFSKAQSADGIITIPLVALLTKAGQLQSLSLREVRSIKILDDRLQRDLE
                     YYLKTYLAAKKKDVKQFTLFAQGTGTRQLQASYTVAMPVWKATYRIILLENSEPTIQG
                     WAVVDNTQDEDWENIHLSLISGLPISFEHDLYTARYLRRPVVHVKEEAAVAPPTMEEG
                     IPTEFSRERSTREIVPPPQSVFDDVMAAPAAVAPAPAPGFAARRAAVSTVQVQTRERA
                     VGDLFQYEITSPVTIKRNQSALVPIVFTKFKGRSVLLYNELTRGRNPLTCVEMENTTG
                     LTLEGGPVTVLDGSTYVGEAMLQTMRPGDNRLIPYSVELGVVADATIDGRKERTHRVR
                     IANGTIEASYLDIR"
BASE COUNT          369 a          362 c          393 g          393 t
ORIGIN      
        1 accctgaacc ctgaaccctc aatttatcct tgactctctt tccgattcag agttgtatat
       61 cgcaacctta tctgaaatca cgtcatttct tttttaaatt actgatttaa ggagttttat
      121 tctgtatgct gaacattcgc cgggtgattt tatacaaaca cggagttgga tattttgaac
      181 gggttggcaa agttgttgac gatcaacggg ttgatctgtt tttccgcgcc tcagaagtga
      241 gcgatgtgct gaaatcactc actgtaattg acctcgatgg tggcacggtg gcctcagtct
      301 cgtatgactc aatcaagcca attgaagaac tactggccga tattgccttt aacattcctg
      361 acgaaggcag tttgctgcga ttgctgccac agttgaaagg cgctgaggtt gaggtccagg
      421 tgggttctga gacgattgtc gggacggtga ttgggattga tttttcaaaa gcccagtcgg
      481 ctgacgggat tatcaccatt cctttggtgg ctctgttgac caaagccggt cagttgcaaa
      541 gtctgagttt gcgtgaggtc cggtcaataa aaatcctcga tgatcgtctg caacgggatc
      601 ttgaatatta tttaaaaacc tatctcgccg cgaaaaagaa agatgtcaaa caatttacac
      661 tctttgccca gggaacaggc acgcgacagt tacaagccag ctacaccgtg gccatgcccg
      721 tgtggaaagc gacctatcgc attattttgc tggaaaattc tgagccgaca atccagggtt
      781 gggctgtggt tgataataca caggatgaag attgggaaaa tattcatctc tcgttgattt
      841 cagggttgcc gatttccttc gagcacgacc tctatactgc ccgctatctg cggcgtccag
      901 tggtgcatgt caaagaggaa gcggcggttg cgccacccac catggaagaa ggaattccga
      961 ctgaattttc ccgtgaacgg tcaacgcgtg aaattgtacc gccaccccaa tccgtttttg
     1021 atgacgtcat ggcagcgccg gctgcggttg ccccagcccc ggccccaggt tttgccgccc
     1081 ggcgagcggc ggtcagtaca gttcaagtcc aaacccgaga acgagcagtt ggcgatttat
     1141 ttcagtatga aatcacctcg cccgtcacca tcaagcgtaa tcaatcggcc ctggtgccga
     1201 tagtttttac taaattcaag ggacgttcgg tgctgcttta taacgaactg acccgaggtc
     1261 ggaacccatt gacctgcgta gagatggaaa ataccacggg gctaacgctt gaaggcggtc
     1321 cggtgacggt tctggatgga agcacctatg ttggtgaagc catgctgcag acgatgcgac
     1381 cgggtgataa ccggctgatt ccatattcgg ttgaacttgg cgtcgtggca gacgccacca
     1441 ttgatggacg caaagaacgc acccatcgcg ttcgaattgc aaatgggacg attgaagcca
     1501 gttatctgga tattcgg
//
LOCUS       DAOQDO010001851         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95084_length_1517_cov_2.997948, whole genome shotgun sequence.
ACCESSION   DAOQDO010001851 DAOQDO010000000
VERSION     DAOQDO010001851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95084_length_1517_cov_2.997948"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PKZ53_23205"
     CDS             <1..963
                     /locus_tag="PKZ53_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43414.1"
                     /translation="RLTEGVAINTTVEDQQFVDEVLWLLSPQLVTAVQTGVAPAASFP
                     RIVEVMPETDTGEYTFGQINHPTELVTQFQQLVSSQNNVVVSFALREVLNALGERYGI
                     DPKEPLGDAIGPEFGWVTMGPERSRIWFLQAHNKVRLLPVIDRYLRTDGAQLQNQSAQ
                     GVEIVKSSHPDQRAAMFLGDFLVFGPYPALLQISDAFQKGKTLVHTSTYQEWRSRPIP
                     QSWMATSLSSDRTIFQETVQALANTQRPQSQAGTAPASSLPTPPGITSSTSVLRPTGI
                     YITRRSILCLSLQLVGATLADTQSAQTTSALTSPSTKIGTSETQ"
     gene            1019..1390
                     /locus_tag="PKZ53_23210"
     CDS             1019..1390
                     /locus_tag="PKZ53_23210"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNJ43415.1"
                     /translation="MLQAEIIAIGSELLTPYRSDTNSLWLTSQLNALGISLRQKTIVG
                     DDEQCLEDALRDALKRSSMIISTGGLGPTEDDITRKVFSRVLKRPLILHQDILDHIRK
                     LFESRNLRASGNTLARKTIRR"
BASE COUNT          389 a          424 c          354 g          350 t
ORIGIN      
        1 cggcttacag aaggtgtggc catcaacacc acggtcgagg atcagcagtt tgtcgatgag
       61 gtgctctggc tcctttcacc acaactggtc accgctgtgc aaacaggggt tgcgccggct
      121 gcttcattcc cccgaattgt tgaagtgatg ccagaaactg acacgggtga gtataccttt
      181 ggccagatca accacccaac cgaactggtc actcaatttc aacaactggt ctcgtcgcaa
      241 aacaacgtgg ttgtcagctt tgcgcttcgg gaagtgctca atgcccttgg ggagcgctac
      301 gggattgatc caaaagaacc actcggagac gcgattggtc ccgaatttgg ctgggtgaca
      361 atggggccag agcgctcacg aatctggttt ctccaggccc acaacaaagt tcgtctgtta
      421 ccagtcattg atcggtatct gcgaactgat ggagcccaac tccagaatca atcggctcaa
      481 ggagttgaaa ttgtgaaatc ttcgcatcct gatcaacggg ccgctatgtt tttgggagat
      541 tttctggttt ttggtcctta cccggctctc cttcaaatca gtgatgcgtt tcaaaaggga
      601 aaaacgctgg tccatacatc gacttatcag gaatggcgat cccggccaat tcctcaatcc
      661 tggatggcga catctctgtc ctcagatcgc accatatttc aagaaacagt ccaggcactt
      721 gccaacacac agcgaccaca atcccaggcg gggacagcac cggcatcatc acttccaaca
      781 ccacctggaa tcaccagttc aacttcggtt ttgcgaccaa ccggaatcta tatcacacga
      841 cgatccatcc tgtgcctgag cctccaactg gttggcgcca ccctggctga cacacaatcg
      901 gcgcaaacga cttctgcttt gaccagtcct tcgactaaaa ttggaacatc agaaacacaa
      961 taatccagaa ttcaaccttt ttttgagaga gtgtcgcggc ttccctgttt caaacactat
     1021 gttacaagct gaaatcattg ctattggctc agaactgtta accccctacc gaagtgatac
     1081 caattccctc tggttaacct cccagctcaa cgcgctaggc atcagtttac gacaaaaaac
     1141 gattgttgga gatgatgaac agtgcctgga agacgcactc cgagatgcgc tcaaacgctc
     1201 atcgatgatt atctcaaccg gagggcttgg cccaacggaa gacgacatca cccgcaaggt
     1261 gttttcacgg gtgctgaaac gacccttgat tctgcatcag gatattcttg atcacatccg
     1321 caaattgttt gaaagccgca acctgagagc ctccggcaac accctggcga ggaagaccat
     1381 tcgtcgatag tgatgtccag ggtgttgcct gagagcctcc ggcaacaccc tggacatcac
     1441 tatcgacgaa tggtcttcct cgcccttgat ggtggaagga aacagcagca gcggcatcga
     1501 agtctccaac aatgtca
//
LOCUS       DAOQDO010001852         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95085_length_1517_cov_2.987004, whole genome shotgun sequence.
ACCESSION   DAOQDO010001852 DAOQDO010000000
VERSION     DAOQDO010001852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95085_length_1517_cov_2.987004"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..728
                     /locus_tag="PKZ53_23215"
                     /pseudo
     CDS             168..728
                     /locus_tag="PKZ53_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            888..1481
                     /locus_tag="PKZ53_23220"
     CDS             888..1481
                     /locus_tag="PKZ53_23220"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ43416.1"
                     /translation="MALGKALTRQTGASRFYLLLVLEQFGNRMVLPSVVEVLNSESDS
                     GIRLAAARTLAAIGDVSEIDVLANAFASEPDPMVQSGMVEALFTLGGIQSVSILVPAL
                     NASESNVRSKVAEALGRLGDQQAVEPLIAQLSDQNNYVRAEVAEALGRLRDPRAKERL
                     ELLQNDPDWNVREKVNAALRQLPGSQKLTIWNRLGWK"
BASE COUNT          351 a          361 c          415 g          390 t
ORIGIN      
        1 tttcagcccg atgttttcag ccctcagccc tcagcccgtt ttcttcagcc ctcaacccgc
       61 tgtccccaga tcttcatatc caaaaaattt gttcgatatt tttgagaatt cagtcatccc
      121 aagtaacaga ccacctcttc aatttatgaa cacttgaggc atttttcatg acgtttctcg
      181 acacttcgcg acgtgaaatc aactgcaaga ttgtctatgt cggtcctggt cttgccggga
      241 aaaccacgaa tttgcaattt ctgtttgatg cgcttccatc taactgcaag ggaaagctgc
      301 ggagtgtggc aactgaaact gaccggacgc tgttctttga ctgtgtcttt ccaaaacccc
      361 tggttgttca ggggtatcgc ctcagatttc atctctacac cgtacctggg tgagtctttt
      421 atgacaccag ctggaggctg gttttaaaag gtgcggatgg ggttgtcttt gtggctgata
      481 gccagccgga acggatggat gccaacgaag aggatctttt caaactggat cggcatttgc
      541 agtcgtttgg ctacagtttt gatgtcattc cctatgcctt gcagcttaat aaacgtgatt
      601 tgcctggagt gatgccggtg caacaaatgg tgacgggtct gcggcggaag ggggaaccgt
      661 acttcgaagc cgtcgcttac aaaggaactg gtgtctggga aacattgcgc tccgttaccc
      721 ggcaaattgc ccacaggctt gcccagcaat caaacccagg gctggccaca atcaagcttc
      781 cagtagatgt tgaatcattg atcaaaaagc tggaaaccac ttcggacccg gaaactgtga
      841 gggaaattgc ttcccggttg gggcaatccg gtgagttgaa agctgtggtg gctctgggaa
      901 aggctctcac ccggcaaact ggggcctcca gattctatct gttgctggtc ctggaacaat
      961 ttggaaaccg aatggtcctg ccatcggtcg ttgaggttct gaattcagag tcggattcgg
     1021 ggattcgcct ggctgccgcc cgaacgctgg ctgcaattgg ggatgtttcc gaaattgatg
     1081 tgcttgcaaa cgcctttgcc agcgaacctg acccaatggt gcagtctggc atggtggaag
     1141 cactcttcac tttgggagga attcaatcag tttccatttt agtgccagcg ctcaatgctt
     1201 ctgagagcaa tgttcgaagc aaagtcgccg aagcgttggg gcgtcttggc gatcaacagg
     1261 cggttgaacc tttgatagct cagctttcag accaaaacaa ctatgttcgg gctgaagttg
     1321 cggaggctct gggaaggttg cgtgacccac gggcaaaaga acggctggaa ttgcttcaaa
     1381 acgacccgga ttggaacgtc cgggagaaag tgaacgctgc gttgagacag ctcccaggca
     1441 gccagaagtt gaccatctgg aatcggttgg gatggaagta agatcatttt gggctgaggg
     1501 tatcgggcta agggctg
//
LOCUS       DAOQDO010001853         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95109_length_1517_cov_2.483584, whole genome shotgun sequence.
ACCESSION   DAOQDO010001853 DAOQDO010000000
VERSION     DAOQDO010001853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95109_length_1517_cov_2.483584"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKZ53_23225"
     CDS             complement(<1..463)
                     /locus_tag="PKZ53_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43417.1"
                     /translation="MKIQWFTVLLLNLVCAGLVLGTNPCLAVTPPVKGEKIKVEDVIA
                     KHVESLGTQKALTDVKTRVIAGTAMFRSKTIGATQLQGPAVVASDGAMSLVGIGFNTA
                     EYSQEKVAYDGKNVTVGYIRPGTRSALGEFLLARNVIFKEGLFGGVYSSGWA"
     gene            complement(893..>1517)
                     /locus_tag="PKZ53_23230"
     CDS             complement(893..>1517)
                     /locus_tag="PKZ53_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43418.1"
                     /translation="KALAEKSDNLSVMAQLADVASNAALNGNLAFSDQGIKFGRDVIA
                     LFENGKRPYEFTEEQWAGVRPKRMAQLYTSVGILLTLKNESDGAVNMLVKALQFEGRD
                     PYTQLMLAKAYKSMLDNLSKGVGKNPAVPKELTGQIQTVREQMMLSYTRALVLSEDPQ
                     FSWLHPSVEAEVTYVYQTVPELAKIQSMTLQALTDRVRTEQNAEPSK"
BASE COUNT          365 a          389 c          369 g          394 t
ORIGIN      
        1 atgcccaccc ggacgaataa accccaccaa aaagcccttc cttaaaaatc acatttcggg
       61 ccagcaaaaa ttctccgagc gccgagcggg tgccgggccg aatatatccc acggttacat
      121 tcttaccgtc ataagccact ttttcctgtg aatattcggc tgtattaaaa ccaattccca
      181 ccaggctcat agcgccatct gaagccacca cagccgggcc ctgtaactgg gtggcaccaa
      241 ttgtctttga tcgaaacatg gccgtcccgg caatcactcg ggttttgaca tcagtcagcg
      301 ccttctgcgt tccaagcgat tccacatgtt tggcaatcac gtcttctact tttatttttt
      361 cccctttgac tgggggagta acggccagac aggggttggt acccaggacc aatccagcac
      421 acaccagatt gagtaaaaga actgtaaacc actgtatctt cataattttt tgcaagctgt
      481 ggagggaaat ctcccagctt tctcctttct caagccgaaa tatgatcggc agtttcccag
      541 ggcaacgccc tccgctcagg gtaggtaaac ggcaaagggg ttttttggtt ggaactgaac
      601 ggcaggtcca gctctttggg cctgggttta tgaaaaagca catttcaggc aatgtgcggc
      661 tagttgttag aacgatcacg ccggcgctcg atcaatggag gaaggtagtc cctcaaacca
      721 ggcacagatt ggaaaccgga tggtgattga gtttgaaatg cacacggaat tgatgaggca
      781 ctcagacgag gcataaacca actatagtat aaaaaacagg acggcacgaa ccccaaactg
      841 gtgattcgtg ccgtcggtgt gttttttctc ggatacggtt ggtttgtcag agttatttgc
      901 tgggttctgc gttctgctcg gtgcgaaccc gatccgtcag cgcctgtaag gtcattgact
      961 gtattttggc cagctctgga accgtctggt agacataggt gacttcggct tcaaccgaag
     1021 ggtgcagcca gctaaactgc ggatcttcgg aaagcaccag ggcccgggta taactcaaca
     1081 tcatctgttc gcgaacggtt tggatctgcc ctgtcagttc tttagggaca gccggatttt
     1141 ttccaactcc ttttgacaga ttatccagca ttgatttgta cgccttggcc agcatcaact
     1201 gggtataggg atcgcgtccc tcaaattgga gggctttgac cagcatattc acggcgccat
     1261 ctgactcatt cttcagtgtg agtaaaatac cgactgatgt atagagttgc gccatgcgtt
     1321 ttggtctgac gccagcccat tgctcttcag taaattcata cggacgtttt ccattttcaa
     1381 acagggcgat gacatctcgg ccaaatttga ttccctgatc agaaaacgcc agatttccgt
     1441 tgagcgcggc gttactggcc acatcagcca gttgagccat cacactcagg ttgtctgatt
     1501 tttcggccag agctttg
//
LOCUS       DAOQDO010001854         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95154_length_1517_cov_1.857729, whole genome shotgun sequence.
ACCESSION   DAOQDO010001854 DAOQDO010000000
VERSION     DAOQDO010001854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95154_length_1517_cov_1.857729"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKZ53_23235"
     CDS             complement(<1..574)
                     /locus_tag="PKZ53_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43419.1"
                     /translation="MNQLARCGIFIILLFQLSCREVESRTGQPSTTTRLPVEVDVLII
                     NTNPNDGFNVPGEITTGESFVVKSPRSGKILEIQHLSDGIIRKGTVLARLNREPLEAQ
                     LTELQLSLRSAELQIQSAEKSVMEHRRTLAQNESLFAGGLQNRNMVEKSRFELEQSVL
                     DEQKARIDKEVAATRVTKTQAELAASDLVAP"
     gene            complement(579..1370)
                     /locus_tag="PKZ53_23240"
     CDS             complement(579..1370)
                     /locus_tag="PKZ53_23240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ43420.1"
                     /translation="MSLSIPEFSAESVSLVDAVRIGRFERKLDWLEKAVNGAVVRRVA
                     QTRGIEISDQDLKPLIEAWRQQYRLSRPEDVTAWLAHCRLSLEDLELCLTEKALEQKL
                     IELEILPQIEPFFKRNRLEYDQVCLGRLKVSSQALAQEVMAQIVDDGTDFAILARLYA
                     LTPDMGFNAGYVGWKYRAELSTEIATAVFGSEDGDIIGPFRQGDGWEIFKIHTIKRAT
                     LDEPTARLIQDRLWEEWIKAEILKQEVTAASTRHQPPAVPNNQVH"
BASE COUNT          346 a          379 c          387 g          405 t
ORIGIN      
        1 acggcgccac gaggtcagac gctgccagtt cagcctgagt tttggtcaca cgggtggccg
       61 cgacttcttt gtcaattcgg gctttttgtt catcaagcac tgattgctca agttcaaagc
      121 gtgatttttc gaccatattg cgattctgta agccaccagc aaagagcgat tcgttttgtg
      181 ccagcgttcg ccgatgttcc atcactgatt tttcggctga ttgaatctgg agttcggctg
      241 acctgagcga aagctgaagt tcggtcaatt gtgcttcgag gggttcgcgg tttaaccggg
      301 ccaaaaccgt tcctttgcgg atgattccgt ctgatagatg ctgaatttcc aggatttttc
      361 ccgaccgtgg cgacttcacg acaaatgatt caccggtggt gatttcacca gggacattga
      421 atccatcatt gggattggta ttgatgatca acacatcaac ttcaactgga agccgggtgg
      481 tcgttgatgg ctgtccggtt cgtgattcaa cctcccgaca actcagttga aacagaagaa
      541 tgataaaaat tccacagcga gcgagctggt tcatagattt agtgcacctg gttgttggga
      601 acggctggtg gttggtgtct ggttgaagct gcagtgacct cttgtttgag aatttctgct
      661 ttgatccatt cttcccagag tcggtcctga atcaagcggg ccgtcggttc atcaagtgtt
      721 gcgcgtttga tggtatgaat tttgaagatt tcccagccgt caccctgccg aaacggaccg
      781 atgatgtcac catcttcgct tccgaaaaca gccgttgcaa tttcggttga taattcggct
      841 cgatatttcc atccgacata tccagcattg aaccccatgt ccggagttaa agcatacagc
      901 cgggccagaa tggcaaaatc agtgccgtca tccacgattt gcgccatgac ttcctgagcc
      961 agcgcctgtg acgagacctt caaccgcccc agacaaacct ggtcgtactc cagccggttg
     1021 cgtttaaaaa atggttcaat ctggggaaga atttccagtt caatcaattt ttgttcaagt
     1081 gctttttcag tcaggcacag ttcaaggtcc tcaaggctca atcggcagtg ggcaagccag
     1141 gcggtgacat cttcgggtct ggaaaggcga tattgttgtc tccaggcttc aatcaatggt
     1201 ttgaggtcct ggtctgaaat ctcgattcct cgggtctgag cgactctccg caccacagcc
     1261 ccgttgacgg ctttttcgag ccaatccagc tttcgttcaa accgcccaat gcgaacagca
     1321 tcaaccaggg aaacagattc agcgctgaat tcaggaatcg aaagtgacat agtgtttagg
     1381 gctgaaggca tcgggttgaa gatatcgggc tgaaaaagtc gggctcaggg ggttcgcccc
     1441 gttctaacgt cccaaccgtc cccaaaccca ttgggcaatc gtccgttttt cagagctgac
     1501 ccaaatggtg gcttcca
//
LOCUS       DAOQDO010001855         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95195_length_1516_cov_3.984942, whole genome shotgun sequence.
ACCESSION   DAOQDO010001855 DAOQDO010000000
VERSION     DAOQDO010001855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95195_length_1516_cov_3.984942"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKZ53_23245"
     CDS             complement(<1..411)
                     /locus_tag="PKZ53_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNJ43421.1"
                     /translation="MTEDQFTHLITRLEDLAQRHPVQYKLRVALVAMLGYAYMVLVLM
                     VLAALIFLMMGLMPTRRSGDPAVYLAIKIGVVVFFLATVLMRAMWVKIPTPNGLKLTR
                     EQTPRLFHLVDELSKKLQTPRIHHILIDIEYNAAM"
     gene            complement(460..1176)
                     /locus_tag="PKZ53_23250"
     CDS             complement(460..1176)
                     /locus_tag="PKZ53_23250"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HNJ43422.1"
                     /translation="MLKSSKLLSVFFLVVLLCAQSLASLASGPSTIRSAGEKETERIN
                     NAATMFDEIMKAPDKGVPGDLLETCAALILIPGVKKGAFIFGGEYGKGVMLVRNPDKS
                     WNIPGFIRMDGGSVGFQIGGQSIDVILVVKNRKGIEKLTNNKFKLGADASVAGGPVGR
                     DAKANTDAQMQAQILSYSRTQGAFAGVSLEGAALRLDDNSNKSVYGRALKADDVLNPS
                     SLKAPAAADELYKVIKKYAK"
     gene            complement(1319..>1516)
                     /locus_tag="PKZ53_23255"
     CDS             complement(1319..>1516)
                     /locus_tag="PKZ53_23255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43423.1"
                     /translation="ATWFQPIASVIDADQWTLVDLRPLRPILASGKIKGLHRNLEKVI
                     WGFDLVVVLSKSQPATPLRKS"
BASE COUNT          367 a          404 c          365 g          380 t
ORIGIN      
        1 catggcggca ttgtattcaa tatcaattaa gatgtggtga atacgtgggg tttggagctt
       61 ttttgaaagc tcgtctacca gatgaaacag ccggggcgtt tgctcgcgcg tcagtttgag
      121 cccattgggg gttgggattt tgacccacat ggcgcgcatc aaaacggttg ccaggaaaaa
      181 aaccacaact ccaattttga tggccagata gacggcagga tcccctgagc ggcgcgttgg
      241 catcaagccc atcatcaaaa aaatcaacgc agccagcacc atcaacacca aaaccatgta
      301 ggcgtatccc aacatcgcca ccagcgccac ccggagtttg tattgcaccg ggtgacgctg
      361 ggcgaggtct tcaagcctgg taatcaagtg agtgaactgg tcttcggtca tgcgtcaact
      421 ttcaacccag gcgcagtccg caaagccgtg cgacgcctgc tacttcgcgt attttttgat
      481 taccttgtac agctcatcag ccgcggcagg agctttgagt gaacttgggt tgaggacatc
      541 atcggctttg agcgcccggc catacaccga tttattggaa ttatcgtcaa gtctgagggc
      601 tgcgccttcg agtgagacac cggcaaatgc cccctgggtg cgcgaatatg acaaaatctg
      661 agcctgcatt tgcgcgtcgg tattggcttt cgcatcacgt ccaaccgggc cgccagccac
      721 cgaagcatcc gccccaagtt taaacttgtt gttggtcagt ttttcaatcc ccttgcggtt
      781 tttcaccacc aggatgacat caattgactg accaccgatt tgaaacccga cgctgccacc
      841 atccatgcgg ataaaccctg ggatattcca gcttttatcc ggatttcgaa ccagcatcac
      901 gccctttcca tattctccac caaaaataaa ggcacctttt ttgacacctg ggatcaaaat
      961 caacgcagca caggtttcga gcaaatcacc tggaacacct ttgtcgggtg ctttcataat
     1021 ttcatcaaac atggtggcgg cattgttgat tcgttcggtt tccttctcac cagcagagcg
     1081 aatggtggat ggacctgaag ccagcgaagc cagcgattgt gcgcacaata acacgactag
     1141 aaaaaaaaca ctgagcaatt ttgaactttt taacatagat cctccaattg agtttgaata
     1201 agagtgtgaa tggaaagcag agtttcgggt tcagggtttc gggtcttcga atttatgtcc
     1261 tttatgtcct tttcgtccct ggtgtccctc ttgtcccttt tgtctttccc tgaaccaatc
     1321 aggattttct cagcggtgtg gccggctgac ttttggacag caccacgacc agatcaaatc
     1381 cccaaataac cttctccaga ttccgatgta gtcccttgat tttaccgctg gccaggatcg
     1441 gacggagcgg gcgaagatcc accagcgtcc actggtcggc gtcaatcact gaggcaatcg
     1501 gctgaaacca tgtggc
//
LOCUS       DAOQDO010001856         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95207_length_1516_cov_3.036961, whole genome shotgun sequence.
ACCESSION   DAOQDO010001856 DAOQDO010000000
VERSION     DAOQDO010001856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95207_length_1516_cov_3.036961"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKZ53_23260"
     CDS             complement(<1..243)
                     /locus_tag="PKZ53_23260"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ43424.1"
                     /translation="MRREAIRKWKSNPSNPVGPFEVNLKSGELYRDSELVSLPPQPFK
                     VLVLLIEHAGELVTREEIQRQVWGTDTFVDFEKGLNF"
     gene            471..1421
                     /locus_tag="PKZ53_23265"
     CDS             471..1421
                     /locus_tag="PKZ53_23265"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ43425.1"
                     /translation="MIKRILFLGLFCLLPVTVFAQNQPPNDDREADRTAIREHIEGIF
                     RAYINKDRKTVQATHSEDWRGFLIGTQHIIRGIDEYMQAADSSLKNPTAGMAKFSFPE
                     FDVIFQGPNLALVCYVADIEGKVGDTPFTDKYRTLDVYARRNGAWIQIASNTARHPEA
                     IAKNFSQPVPVTPQTRQSILDAREEVWRAWFSNDQAKLEKLIPPEVIAINGGSETWAN
                     RNQILASARQLAAKGTKLIRLEFPRTDLQIFGRTIILYTTYVYETETDGKRTTVSGRG
                     TEMFVIRDNTLVNVGWHLENDGQELDKSQGSAVEKEKPTQ"
BASE COUNT          403 a          381 c          365 g          367 t
ORIGIN      
        1 aaagttgaga cctttctcga aatcaacaaa tgtgtctgtc ccccaaacct gacgctgaat
       61 ttcctcacgg gtcaccaact ctccagcatg ttcgatcagc aagacgagta ccttaaacgg
      121 ctgtgggggc agactgacca gttcactgtc ccgatacagt tctccgcttt tcaagttgac
      181 ttcaaacgga ccaaccggat tggatgggtt tgatttccat ttgcgaatag cttcacggcg
      241 cacactcctc ttcctcagtt gatgggaaaa ccatctcgca ctgaggtgtt caggtttggg
      301 atgtgtcggc gtggagcatt cattctaaag cggttgccct tattcaaaaa gaaatctcat
      361 tcaaatctca caaaattctc atcggttgct ggttgggatg aagttcagtt cccggttaca
      421 aacctggcgc acattgccaa cctgtttgtt tcatcgaaag gagactgacc atgatcaaaa
      481 gaattctatt ccttgggctg ttttgcctgt tgccagtcac ggttttcgct caaaaccaac
      541 ccccgaatga tgaccgggag gctgatcgga cagcgattcg agagcatatc gaaggtattt
      601 ttcgggcgta catcaataaa gatcgcaaga cagtgcaggc gacccattcg gaagactggc
      661 gaggctttct catcggcaca cagcacatta tccgtggaat tgacgaatat atgcaggcgg
      721 ctgacagttc attgaaaaac ccaacggctg ggatggcgaa attttcattc cctgaatttg
      781 atgtgatttt tcagggaccc aacctcgctc tggtgtgcta cgttgcagat atcgaaggaa
      841 aagttggaga taccccgttt accgacaaat accggacact tgacgtctat gccagacgca
      901 atggagcgtg gatccagatt gcctccaata cagcacgcca tccagaagcc attgccaaaa
      961 atttctccca gccggtacca gttacaccgc aaacacgtca gtccattttg gatgcccgcg
     1021 aagaagtctg gcgggcgtgg ttttcaaacg atcaggcaaa gctggaaaaa ctcatccctc
     1081 ccgaagtcat tgcgatcaat ggtggatcag aaacctgggc caaccggaat cagatactgg
     1141 caagtgccag acaactggct gccaaaggta ccaaactcat tcggctcgaa tttccacgga
     1201 ctgacctgca gatttttggc cggacaatca tcctgtacac gacttatgtg tatgaaaccg
     1261 aaaccgatgg caaacgaacg accgtcagtg ggcgagggac agaaatgttt gtgattcgtg
     1321 acaatacgct ggtcaatgtc ggctggcatc ttgaaaacga cggacaggaa ttggataaaa
     1381 gtcaggggtc ggcggttgag aaggagaaac caactcaatg atttgttttc gaaacccttg
     1441 agctttcccc gacaccactc attcttcttc ctgaaaacaa ccgcattttg gagtgcggcg
     1501 gctcgacgcc gctttt
//
LOCUS       DAOQDO010001857         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95217_length_1516_cov_2.765229, whole genome shotgun sequence.
ACCESSION   DAOQDO010001857 DAOQDO010000000
VERSION     DAOQDO010001857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95217_length_1516_cov_2.765229"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..669
                     /locus_tag="PKZ53_23270"
     CDS             196..669
                     /locus_tag="PKZ53_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ43426.1"
                     /translation="MHRQSILFRLNLRYVPIIGSLLVVATLFTSVQAQNRAARHGQFH
                     IEWKDQHTQQPIEKARVEICPLLEGPGIILETDAKGRASHIRLPEGGYVVFLFREGYA
                     PVEISDVVIEDHYPVRVYVKSLNADEAPYKRKFIRYQRSQVQTDSSEIRTIMRSQ"
     gene            complement(913..1188)
                     /locus_tag="PKZ53_23275"
     CDS             complement(913..1188)
                     /locus_tag="PKZ53_23275"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ43427.1"
                     /translation="MNKAELVELIAQDAEISKAAAEKALNAILSGIADGLRNGKDVTL
                     PTLGSFKLITYKPRRTINPQTKRPMQLPERKSVRFAVNTRLKNALNR"
     gene            1247..>1516
                     /locus_tag="PKZ53_23280"
     CDS             1247..>1516
                     /locus_tag="PKZ53_23280"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNJ43428.1"
                     /translation="MTNFCPKCGTQQIPTNLLCFKCGALLPTSRPQPPQSPPQASLNP
                     YIAPPPDQLTPGYPPPLPQTVNKSRATTSMILGLLSVPLLLLWGLG"
BASE COUNT          356 a          404 c          358 g          398 t
ORIGIN      
        1 ggaagttgat ttttaacgct ccctaaggtt atttgattga agcacttgac tgtttatgaa
       61 tacgagtgtt tcattccacc tgagtatcaa ttgcttttca gcaaccaaat cacagtcatc
      121 ggtccaggcc atatcctacc cgttcagtca ctttgacgtc ggcgggaccg gtttttccca
      181 ccaggaggtt gagcgatgca ccgccagagc attttgttca gattgaatct tcggtacgtg
      241 ccgatcatcg gcagtctcct tgttgtcgcg acgctgttta cctcagttca ggcccaaaat
      301 cgagcagcac gccacggtca gttccatatc gaatggaaag accagcatac ccagcaaccg
      361 attgaaaaag cgagagttga gatttgtccg cttttggaag gaccaggcat catcctggag
      421 actgatgcca aaggccgggc gtcgcatatc agactgcctg agggtggata tgtcgtgttt
      481 ctgtttcggg agggatatgc cccggttgaa atatctgacg tggtcattga agatcactat
      541 ccggtccggg tctatgtgaa atcactgaat gcagatgaag ctccgtacaa acgaaagttc
      601 atccgctatc aacgctccca ggtccaaacc gacagcagtg aaatccgaac cattatgcgc
      661 agccagtaaa cacctggggc ggaaaaaacc agggctgaag aacctgggct tggggctgag
      721 ggctgaagaa aacgggctaa gggctaaggg cttggggctg aaaaaccttg ggctgagggc
      781 ttgaggctca tggtatacaa attatttgaa ttcaattggt ttgtcccctc agccccaagc
      841 ccttagcccg ttttcttcag cccgctttct tcagcccgaa gtcttcagcc ccaggccctt
      901 agccccaaat catcaccggt tcagtgcatt tttgagccgg gtattgacag caaaccgaac
      961 tgatttccgc tctggaagct gcattggtcg tttggtttgg ggattgatcg tccggcgtgg
     1021 tttataagta atcaatttga aactgccaag tgtcggcaac gtcacatctt tgccattgcg
     1081 taacccatca gcaataccac ttaagatcgc attgagtgct ttctcggctg ctgcctttga
     1141 gatttcggca tcctgtgcaa ttaactcaac cagttcagct ttattcatga aatgatcttt
     1201 ttcctttctg cgtgcgctca gttcgcacgt ttttcgagtt attcggatga caaatttttg
     1261 ccccaaatgt ggaacccaac aaatcccaac gaacctgctc tgttttaagt gtggtgcctt
     1321 gctcccaact tctcgaccac agccaccgca atcaccaccc caggcaagtc tcaatccgta
     1381 tatcgccccg ccgccagacc agttaacccc agggtatccg ccgccattgc cacagaccgt
     1441 taacaaatca cgagccacga cctctatgat tcttggctta ttgtcggtcc cgctcctcct
     1501 tttatggggg cttggc
//
LOCUS       DAOQDO010001858         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95259_length_1516_cov_2.093771, whole genome shotgun sequence.
ACCESSION   DAOQDO010001858 DAOQDO010000000
VERSION     DAOQDO010001858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95259_length_1516_cov_2.093771"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1516)
                     /locus_tag="PKZ53_23285"
     CDS             complement(<1..>1516)
                     /locus_tag="PKZ53_23285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ43429.1"
                     /translation="VYTNQLHICLDDRLSWLAAYGFDAAVMDIFGALLNGATLCPFDV
                     RTEGLDRLPAWVVDQRISIYHSTPTVYRYFVATLTPETNLTLLRLVVLGGEEVVKRDF
                     DQFVEHFPDHCLFVNGLGPTESTITLQQFMNRESMVSGSTIPVGFPVPETQVLVLNSD
                     GEPVAPLAAGEIVIRSPFVALGYWHRPDLTQKAFAVDDKDSRFRRYRTGDIGRFQPDG
                     TLEFMGRRDFQVKIRGHRIELNEIEARMLTCSWVKEAVVVAIPVSSEEKRLAGYVVPR
                     AGFPVDLTGLRAMLKATLPAPMVPAAITVLEALPLTPNGKLNRHALPVPDWTEGISSR
                     QMVSPRTSTEAALERIWAKILGQPQVGIHDNFFDLGGHSLLAVRLRAEIKQQFNQDFP
                     LALLFQYSTIEQLARCLDQTVSETVWSPLTPIQPEGQRTPFFCVHPAGGNVLGYADLA
                     RRLGTDQPFWGLQATGLDGRQAPLTSVAEMANRYVLALREVQPSGPYVLGGASMG"
BASE COUNT          360 a          440 c          409 g          307 t
ORIGIN      
        1 cccattgacg ccccgccgag aacatagggg ccgctgggct gaacttcacg cagggccaac
       61 acataccggt tggccatttc cgcaaccgag gtcaacggcg cctgccggcc atccagcccg
      121 gttgcctgaa gcccccaaaa tggctggtca gttcccaggc ggcgagccag gtcggcatat
      181 ccgagcacgt ttccaccggc tggatgaaca cagaaaaacg gcgtccgctg gccttcgggc
      241 tgaatcggcg tcaatggtga ccagacggtt tcggaaacgg tttgatccag acagcgggcc
      301 agttgctcaa tggtcgaata ctggaacaac aacgccagcg gaaaatcctg attgaactgc
      361 tgcttgattt ccgcccggag ccggactgcc agcaatgaat gcccacccag atcaaagaaa
      421 ttgtcgtgga tgccaacctg tggctgccct agaattttcg cccagatgcg ttccaaagca
      481 gcttcggttg aggtgcgtgg gctaaccatc tggcgtgagg aaataccttc agtccaatct
      541 ggaaccggca gggcatgacg attgagttta ccgttaggcg ttaacggaag cgcctcaagg
      601 acggtaatcg ctgccggcac catgggtgcc ggtaaggtag ctttgagcat cgcgcgcaac
      661 ccggtcaggt ccactggaaa tccagcgcgt ggaacaacat acccggccag tcgcttttct
      721 tcgcttgaaa ccggaattgc caccaccacg gcttctttca cccaggagca agtcagcatt
      781 ctggcttcga tttcgttcaa ctcaattcgg tgacctcgaa ttttgacctg gaaatcccgc
      841 cgccccataa actccagcgt tccatctggt tgaaagcggc caatgtcgcc ggttcgatag
      901 cgtcggaaac gtgaatcctt gtcatccacg gcaaatgctt tttgggtcag gtcaggccga
      961 tgccagtatc cgagcgccac aaacgggctg cgaatcacaa tttccccggc tgcgagcggg
     1021 gccacgggtt cgccatcgct attgagcacc agtacctggg tttcagggac cggaaagcca
     1081 accggaatgg tgctcccgga aaccatactt tcgcggttca taaactgctg caatgtaatg
     1141 gttgactcgg tggggccaag cccattcaca aacaaacagt gatctggaaa atgttcgaca
     1201 aattgatcaa agtcgcgttt gaccacttct tcgccaccca gtaccacgag ccgcaggagt
     1261 gtcaggtttg tttcaggtgt cagcgtggca acaaaatagc ggtacaccgt tggtgttgag
     1321 tggtaaatgg agatgcgctg gtcaaccacc caggccggca accggtcgag cccttcggtg
     1381 cgcacatcaa acggacacaa tgtcgctccg ttgagcagtg ctccaaaaat atccatcacc
     1441 gccgcgtcaa atccataagc tgccagccac gacagccggt catccagaca aatatgaagc
     1501 tggttggtat aaaccc
//
LOCUS       DAOQDO010001859         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95436_length_1514_cov_3.399589, whole genome shotgun sequence.
ACCESSION   DAOQDO010001859 DAOQDO010000000
VERSION     DAOQDO010001859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95436_length_1514_cov_3.399589"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKZ53_23290"
     CDS             <1..544
                     /locus_tag="PKZ53_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNJ43430.1"
                     /translation="GNSEHCVAVHPSDMCVALAALDAVVEVEGPKGQRKIAFGEFHRL
                     PGDTPNRDTNLEPGELITAVTIPASPFAKSSVYLKLRDRASYAFALISVAAVLDVSGK
                     KIKTARLALGGVAHKPWRTTEAEAFLQGKSADTATFQKAAELALKGAKPLSHNEFKVT
                     LAKRAIVRALTISAKGGGVA"
     gene            541..>1514
                     /locus_tag="PKZ53_23295"
     CDS             541..>1514
                     /locus_tag="PKZ53_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ43431.1"
                     /translation="MTKVIGTSVSRTDGLAKVTGTATYAAEHQIPGLVHGYLVTASIS
                     AGRIKNIDTKEAEKAPGVIAVFTHRNPPKMFMPANDFMNSRIYEARLPLSDDKVHYAG
                     QIIGVVVADTLERARHGAHLVKVDYDMAKPVVSGRDTSYKDASSMFGEELKFQKGSFE
                     TGTFAAGSASAAAKVDAVYGTATELHSPMEPHAIISHWHSDGSLTVYEPSQWVYGTQR
                     TYADLFGIAPEKVRIVTPFIGGGFGSKAFPWQHSVLCAAAGRAIDRPLKLVVTRRQMT
                     ANTGHRSETEQTVRLAATADGTLTAIEHLAKTSTSPVETFAEPCTGITP"
BASE COUNT          372 a          409 c          415 g          318 t
ORIGIN      
        1 tgggaatagc gagcactgtg tggcggttca tccgtcagat atgtgtgtgg cgttggcggc
       61 actggatgcg gtggtcgaag tcgaggggcc aaaaggacag cgaaaaattg ccttcggtga
      121 gtttcatcgg ctgccgggtg atacgccaaa ccgggacacc aatctggaac ccggcgaact
      181 catcacggcg gtgacaattc cagcctcacc attcgccaaa agcagcgtct atctcaaatt
      241 gcgggatcgg gcgtcgtatg cgtttgccct gatttcagtc gccgctgtcc tggatgtgtc
      301 tggcaagaaa atcaaaacgg cccggcttgc cctgggcggc gtggcccata aaccctggcg
      361 cacaaccgaa gccgaagcct ttttacaggg taaatcagcg gatacagcca cattccagaa
      421 agcagcggaa ctggcgctca aaggcgcgaa accactgtct cataatgaat ttaaggttac
      481 tctggccaaa cgggcgattg ttcgcgcatt gacgatttcg gccaaaggag gaggtgtcgc
      541 atgaccaaag tgatcggaac ttccgtcagc cgaaccgatg gattggccaa agtcaccggc
      601 acggccacat atgctgccga gcatcaaatc cctggactgg ttcatgggta tctcgtaacc
      661 gccagcattt cggcgggtcg aatcaaaaac attgacacca aagaggctga aaaagcccca
      721 ggcgtgatcg ccgtctttac ccatcgcaac ccacccaaaa tgtttatgcc agccaatgac
      781 tttatgaatt cacgaattta tgaagcccgg ctgccgctct cagatgataa agtccactat
      841 gccggtcaaa ttattggggt tgtggtggct gatacgctgg aacgagcccg tcacggggcc
      901 catctggtca aggttgacta tgacatggcg aaacccgtgg tatcaggcag ggacaccagt
      961 tacaaagatg cgtcctcaat gtttggcgaa gaactcaaat ttcaaaaagg gagttttgaa
     1021 actggaacgt ttgcggctgg ttcagccagc gctgctgcca aagttgatgc cgtttacgga
     1081 acagcaaccg aacttcattc cccaatggag ccgcacgcca tcatcagcca ctggcattcg
     1141 gacggttcgt tgaccgtgta tgagccatct cagtgggtct atggcacaca acgaacctat
     1201 gccgatttgt ttggaattgc ccctgaaaaa gtgcggattg taacaccttt catcggtggt
     1261 ggattcgggt caaaggcatt tccctggcag cattcggtgt tgtgcgccgc cgccggacgg
     1321 gccattgatc gcccactgaa actggtggtc actcgccggc aaatgacggc caatacaggg
     1381 catcggtcag aaacagaaca gacggttcgg ctggcagcca ccgctgatgg taccctgacc
     1441 gcgattgaac atctggcgaa aacctcgacg tcgccagttg aaacctttgc cgaaccctgc
     1501 accggcatca cacc
//
LOCUS       DAOQDO010001860         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95482_length_1514_cov_2.170665, whole genome shotgun sequence.
ACCESSION   DAOQDO010001860 DAOQDO010000000
VERSION     DAOQDO010001860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95482_length_1514_cov_2.170665"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="rfbD"
                     /locus_tag="PKZ53_23300"
     CDS             complement(<1..677)
                     /gene="rfbD"
                     /locus_tag="PKZ53_23300"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNJ43432.1"
                     /translation="MKILLLGNTGQLGWELNRCLLPLGDVQAMDYPDINLAQPETLRS
                     LIRAVSPDVIVNATAYTAVDKAETERELNFSINAVSPGVMAEEAKKHGAVLIHYSTDY
                     VFDGSKGSLHMETDVTNPLNAYGESKLAGEKAIQDVDGNYLILRTAWVYSNRRDSFVS
                     KVLQWSRQNEVLKIVNDQVSNPTWARMLAEVTSLLLARGTAYIEERKGLYHLAGGGYA
                     SRLDWAR"
     gene            complement(674..1514)
                     /gene="rfbA"
                     /locus_tag="PKZ53_23305"
                     /pseudo
     CDS             complement(674..1514)
                     /gene="rfbA"
                     /locus_tag="PKZ53_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698069.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
BASE COUNT          418 a          421 c          302 g          373 t
ORIGIN      
        1 tcacgcgccc aatccaaccg gctggcatac ccgcctccag ccagatgata caatcccttg
       61 cgttcctcaa tataagctgt gccacgcgcc aaaagcaaac tagtcacttc agccagcata
      121 cgagcccagg tagggttgct gacctggtcg ttcactattt tcaatacctc attctggcgc
      181 gaccattgca aaactttcga cacaaaactg tcacgtcggt tgctatagac ccaggccgtg
      241 cgaaggatca aataatttcc atccacgtcc tgaatggctt tttcaccggc cagtttgctt
      301 tctccatatg cattgagcgg gttggtcaca tcggtttcca tatgtaatga tcccttggag
      361 ccatcgaaca cataatcagt ggaataatgt atcaacactg cgccatgctt tttggcttcc
      421 tctgccatca cacctgggct gactgcatta atggaaaagt tcaactcgcg ctcagtctcg
      481 gctttatcca cagccgtgta tgcagtcgca tttacaatca catctggcga aacagcccgg
      541 atcaagctgc gcaatgtttc aggttgagcc aaattaatat ccggataatc catggcctga
      601 acatcaccca aaggaagcag gcagcgattc aattcccaac ccaattgacc cgtgtttccc
      661 agcaacaata tcttcattca attcttcctt gtgccagtcg atttaaatac tcaccatatg
      721 aattattctt gtactttatt gccaaatcac tcaactgatt gcgatcaata tatcccatcc
      781 gaaaagcaat ctcttccggg caagagatca tcattccctg acgttcctgc accgcctgga
      841 taaaggtcga agcttgcagt aaagactcat gcgtacccgc atccagccag gcatagccgc
      901 gtcctaaaat ttcgacactg agctgcccac gttccatata gatacgattt aaatctgtaa
      961 tttcaagttc accgcgggca gaaggcttga gattggcagc catttccaca acttgcgagt
     1021 cgtaaaaata cacacccggt acagcataat gcgaccgagg ttgacgcggt ttttcttcca
     1081 aacttatcac ctttcctgaa gaatcaaact ccacaacccc ataccgttca gggtcacgga
     1141 ctgggtatgc aaatactcgt gcgccttgct caacttgcat agcatgttga aggatctggg
     1201 caaaccatga ccaaaaaaaa tgttatcgcc aaggattaga caactggaag aaccagccac
     1261 aaagtccgcc ccgacgcgga acgcatccgc cagacctcga ggttgatcct gttccttata
     1321 ttcaaaccga atcccccact gctccccact acccaaaagg cgtttaaaca atggcaggtc
     1381 attgggtgta cttatcagca aaatctcccg aataccggtc aacattaaca ttgaaagcgg
     1441 ataataaatc atcggcttgt cataaacagg taaaagttgc ttgctgactg ccagagttaa
     1501 tgggtgcagg cggg
//
LOCUS       DAOQDO010001861         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95501_length_1514_cov_1.828650, whole genome shotgun sequence.
ACCESSION   DAOQDO010001861 DAOQDO010000000
VERSION     DAOQDO010001861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95501_length_1514_cov_1.828650"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKZ53_23310"
     CDS             complement(<1..248)
                     /locus_tag="PKZ53_23310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43433.1"
                     /translation="MNLDTPSSKAEILATLSQINSTDFQFWLGFSPAAFVEPLEGGWS
                     ASDTVRHLEKSTKPVVKALHLPGLVLRATFGMAPNPSIS"
     gene            594..>1514
                     /locus_tag="PKZ53_23315"
     CDS             594..>1514
                     /locus_tag="PKZ53_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNJ43434.1"
                     /translation="MYVALSGSDTDTCGSAESPCRSIRQGINRATSGQTIAVRAGLYE
                     ESYLELKSNTTLVSADGPLQARIFSGETSAIRFEGISNATLDGFEVFASYDQGPEGDG
                     LVRVLDAANCTIRNCLLHDAAHDSDVVKVSGNVTGLLLENLIAFNPGRRASPNPCDET
                     RPWFQEVIDIYGSGTPGAPPPVDDVIVRGCWLFQTPERGGDYLLYSKINCQNILYENN
                     IFGPSVGQGCGNTAVGIGTSEPGEPDPDAAVVRHAIVRNNIFVGCQGDGVFGIKNSED
                     VWVYNNTFFANGGPNLRSVIELRGNSHALGP"
BASE COUNT          347 a          369 c          416 g          382 t
ORIGIN      
        1 gaaattgatg ggttcggcgc cattccaaac gttgctcgca gcacaagccc aggcagatga
       61 agcgctttca ccactggttt cgtggatttt tcaaggtggc gaaccgtgtc agacgccgac
      121 catccgccct caagtggttc aacaaaggca gccggtgaaa agccaagcca gaattgaaag
      181 tcagttgagt taatctggct gagtgtggct aaaatttcgg ctttggagga cggggtgtcc
      241 aggttcatag agtctccaga tgggtggttt caggttttgc agttgctgtt ggggagaaga
      301 tcggtacaga aagcagggca attctaacgg tgaacccgat attggacaag tttgtctggg
      361 gcctgaattt tttcgctgta ccatctatac tgaaagttca gagttgccca actcattctg
      421 tttttgtcct cacggaattt gtgcttatga agtatttaag acctcacgta caactcacgt
      481 ttgttcaaaa gcttacaggc tgtattttct tgtgcaccat tcttttgaca agtttccaac
      541 tgtttgaccc tgtcgttgaa gcgcaaccac catcaccagc gcagaataca acaatgtatg
      601 ttgctctgtc cgggagcgac actgacacct gtggcagtgc tgagtccccc tgccggtcta
      661 tccggcaggg catcaatcgg gcaacgagcg ggcagacgat tgccgttcgt gccgggctct
      721 atgaagaaag ctacctggag ttaaaaagca acacgacgct ggtctctgcc gatggaccgt
      781 tgcaagccag aattttttca ggcgaaacca gtgccatccg gtttgaggga atttcgaatg
      841 cgacactgga tggcttcgaa gtctttgcca gctacgatca gggccctgaa ggtgacgggc
      901 tggtgcgggt gctggatgcc gccaactgca cgattcgcaa ttgtttgctc cacgacgcag
      961 cccatgactc agatgttgtc aaagtttctg gaaacgttac cggtttgctg cttgaaaacc
     1021 tgattgcctt caacccaggc cgccgcgcat cacccaatcc gtgtgatgaa accaggccct
     1081 ggtttcagga agtgattgat atttatggca gcggaacgcc cggtgcgcct ccgccagtgg
     1141 atgacgttat tgtgcgtgga tgctggctgt ttcagactcc ggaacgcggc ggtgactatt
     1201 tgctctatag taagatcaac tgccagaaca ttttgtatga aaataacatc ttcggtccgt
     1261 cagttggtca agggtgtggg aataccgccg tcggcatcgg caccagcgaa cccggcgaac
     1321 ctgacccgga tgcagcggtt gtccggcacg cgattgtccg taacaacatt tttgttgggt
     1381 gtcagggaga cggcgtcttt ggaattaaga attcagaaga tgtctgggtc tataacaaca
     1441 ctttttttgc caatggcggc ccaaacttgc ggtcagtgat tgagctacgc ggcaattcgc
     1501 acgcgctcgg accg
//
LOCUS       DAOQDO010001862         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95540_length_1513_cov_3.506173, whole genome shotgun sequence.
ACCESSION   DAOQDO010001862 DAOQDO010000000
VERSION     DAOQDO010001862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95540_length_1513_cov_3.506173"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKZ53_23320"
     CDS             <1..204
                     /locus_tag="PKZ53_23320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43435.1"
                     /translation="FCPELASLQPQAPGPGMFSPQCVRWEIKNGANLTKLLQTNTLLA
                     RLKEAASQKNHGQGSSIEEMQEQ"
     gene            821..>1513
                     /locus_tag="PKZ53_23325"
     CDS             821..>1513
                     /locus_tag="PKZ53_23325"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNJ43436.1"
                     /translation="MPLNHFVETHQDSWFDRLKESIKSVATGAFMFLIAFPILFFNES
                     NAVKTSQGLSEGAGSVVKATADQVSPANDGKLVHVTGQVKVEPPPTDVDFGVSAPAVK
                     LRRVVEMYQWKEYQKSEKSKNTGGSSTTTTTYDYKQEWSSDVIKSKDFRIPSGHVNPE
                     KMPFESETIKAESVTVGAFQLSSELTDKMDKFEPLPANETVLSQMPAALRQYAKVSNG
                     AVYYGKNPNSPQI"
BASE COUNT          442 a          364 c          347 g          360 t
ORIGIN      
        1 ttctgccccg agcttgcgag tcttcaaccc caagccccag gcccagggat gttcagccct
       61 caatgtgtga gatgggaaat caagaacggt gcaaatctga cgaagttgtt gcagacaaac
      121 acactgctcg cccggttgaa ggaagccgca tcgcagaaaa accacggcca ggggtcgagc
      181 atcgaggaaa tgcaggaaca gtgatgggtg agtaacgtcc tacgtgaagc gcatgtgtgt
      241 ttctgtaact ccttgttctg tcagaacgcg ccctatggga ttcgaaccca caacacagtg
      301 ttttagagac acctgctcgg ccattgagct tagggcgctg attgtggagt ggaacgaact
      361 ggacaatagc gaaaattttc gaatttgaca aaaatttttt tgagaggccg tatctttttt
      421 gatcttccgg gtaataagcg aatgtcacgc catttccgat ttgccgccga tgatgtgacg
      481 tgctctctca tttcacgctt tccttgatcg tgtggcaaat cggcaatgga cggtacaccg
      541 aaatgaagaa tgaagaattg ggaatgaaga atgaagaaaa tagtactatt ttgtaatcct
      601 tgtattatag agaggctaaa tcattcaaaa acttgtactt cccaatattt gagccataca
      661 tgacataaaa ctgtcattca ttcatcactg cgagcagata ccaggtattt gcttttactt
      721 cactatttta gctacttcca caatcttgga aaaccagaaa ccggaaagta cggaacccgg
      781 accagcatac gtccgccaac tgcgcgaagg agaatttcac atgccactca atcactttgt
      841 tgaaactcat caagattcct ggtttgaccg tttaaaagaa tccatcaaaa gcgttgcaac
      901 tggtgcattt atgttcctga ttgcttttcc gattctgttt tttaatgaaa gtaatgctgt
      961 taaaacctcc cagggacttt cagaaggtgc cggctcggtg gtgaaagcaa ccgccgatca
     1021 ggtaagcccg gccaatgacg ggaaactggt tcacgtgacc ggacaggtca aagtcgaacc
     1081 accaccaacc gatgtggact ttggtgtctc ggccccggca gtcaaactcc gccgggtggt
     1141 tgaaatgtat caatggaagg aatatcagaa atccgaaaaa tcaaagaaca ccggcggaag
     1201 ttcaacgacg acgaccacct atgattacaa acaggaatgg tcaagcgatg tgattaagtc
     1261 caaagacttt cgcattcctt ccggtcacgt caaccctgag aaaatgccat ttgaaagcga
     1321 aaccatcaaa gctgaatcgg ttacagtcgg agccttccag ctctcatctg aattgaccga
     1381 caaaatggac aaatttgaac cactcccagc caacgaaacc gtgctcagcc agatgccagc
     1441 agcgcttcgt caatatgcca aggtgtcaaa cggagccgtc tactatggga aaaaccccaa
     1501 ctcgcctcaa atc
//
LOCUS       DAOQDO010001863         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_95823_length_1511_cov_2.260302, whole genome shotgun sequence.
ACCESSION   DAOQDO010001863 DAOQDO010000000
VERSION     DAOQDO010001863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95823_length_1511_cov_2.260302"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PKZ53_23330"
     CDS             complement(<1..1401)
                     /locus_tag="PKZ53_23330"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ43437.1"
                     /translation="MTTTKCAVIGLDGATFDLILPMVEAGELPTLGRLLNDGSWGTLR
                     STILPNSFPGWASATTGTSEGMHGIFSPFLKRREEYGVRAISAVDLQTRPIWDLLNAQ
                     GYSTGIINIPTTYPVEKVTGYQITGMLTPSTKSPFTYPPSLQAELLEHLPNYIIEPRR
                     ALGRAEKAAEFRNCLEGHVQAAEYLIQTRPTDFLMIVLSVPDRAQHDFWADMDSAHFR
                     YDPVQSPEYSEFIREIYRQADAGVERILRQLPDDVTVVICSDHGFSAELRELRVNEWL
                     AQRGFLRYQPEAFRTASTTLDKISNKLTYHYRALSTKLKPHSVLEKKVVFGRDFLNQI
                     DWEHTTAFFGQDKGVWINLKGRDRAGTVEPQDFERVRSEVIQALNEWIDPETGECVFE
                     AVVPREATLTGRFADRLPDILVIPQRSEDVPIETASPGAPIVPATTTTGTHAPDGILL
                     MAGPHIRKNHQLTGANL"
BASE COUNT          356 a          400 c          386 g          369 t
ORIGIN      
        1 gagattggca ccggtcaact ggtggttttt acgaatgtgc ggccccgcca tgagcagaat
       61 accgtctggg gcgtgcgtgc cagtcgtggt ggttgccggg acaatcggtg ctccggggct
      121 tgccgtttca atcggcacat cttctgatcg ttgtggaatg actaaaatat ccggcaaccg
      181 gtcagcaaaa cgtccagtta aggtagcttc gcgcggtaca acggcttcga aaacacattc
      241 cccggtttca gggtctatcc actcgttcaa cgcctggatc acttcactac gcacccgctc
      301 aaagtcctgc ggctcaaccg ttcctgcccg atcacggccc ttgaggttga tccagactcc
      361 cttatcctgt ccgaaaaacg cggttgtatg ttcccagtca atttgattga gaaagtcccg
      421 gccaaaaacc acttttttct ctaacaccga gtgcggcttg agtttggtag ataaagctcg
      481 atagtgatag gtcagtttat ttgagatttt gtccagcgtc gtgctggctg ttcgaaaggc
      541 ctcaggctgg tagcggagaa acccacgctg ggcgagccat tcattgactc gcaattcccg
      601 cagttcagcc gaaaacccat gatcggaaca aatcacaacc gtcacatcat ctggcaattg
      661 gcggagtatc cgctcaacac cagcatctgc ctgtcgatag atctcacgga taaactcact
      721 gtattcaggt gattgcaccg ggtcataccg aaaatgagcg gaatccatat ctgcccagaa
      781 atcatgttgg gcacgatctg gcacactgag cacaatcatc aagaaatcag ttgggcgagt
      841 ttgaatcaaa tattcggcag cctggacatg gccttccaga caattgcgga attcggcggc
      901 tttttcagct cgtccaagtg ctcgccgggg ttcgatgatg tagtttggaa gatgctccaa
      961 caactcagcc tgaagcgaag gcggataggt aaatggcgac tttgtactgg gagtcagcat
     1021 tcccgtgatc tggtacccag ttaccttttc aaccggatag gtggttggaa tgttgataat
     1081 tccggttgaa tagccttgcg cattcagcaa atcccagata ggccgggttt gcaggtcaac
     1141 cgctgagatc gcccgaaccc catattcttc acgacgcttg agaaatggcg aaaaaatacc
     1201 gtgcatccct tccgaggtgc cagtcgtggc gctggcccaa ccaggaaagc tattcggcaa
     1261 aatggttgat cggagcgtcc cccagcttcc gtcgttgagc aatcggccca gggttgggag
     1321 ttctccggct tccaccatcg gcagtattaa atcaaaggtg gcgccatcca ggccgatgac
     1381 agcgcatttt gtagtcgtca tagttcaggg tcttcagaag agatgaggga tgaaagaaat
     1441 caccctgtca cctcatcacc ttgtcacttt gtcactttgc tatcttcgct ccatcgctgt
     1501 tcgagacatt c
//
LOCUS       DAOQDO010001864         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96008_length_1509_cov_3.199450, whole genome shotgun sequence.
ACCESSION   DAOQDO010001864 DAOQDO010000000
VERSION     DAOQDO010001864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96008_length_1509_cov_3.199450"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..531
                     /locus_tag="PKZ53_23335"
     CDS             181..531
                     /locus_tag="PKZ53_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43438.1"
                     /translation="MVRIISGSSVVLSLHSPREKIWGILVSIEQSGVQICGVDINTFD
                     DWLNSVTNGESNIGLTNSFLPMWRVERISLDETIGDILSLEEQFALRVGITLTEYLKG
                     AKDGYVSSTENWRQ"
     gene            complement(675..1175)
                     /locus_tag="PKZ53_23340"
     CDS             complement(675..1175)
                     /locus_tag="PKZ53_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ43439.1"
                     /translation="MAQERKKRPKRYTIGVVANTFGIHEQTLRLYEREGLLTPSRSEK
                     NTRYYTDDDLEKLKMILSLTRDMGVNLAGVQIIIDLRQRMDEMQRDFNRFLEYLREQI
                     AENMAVDQEHLQNALVRTPPSHLVIIEQTERRTLRGSTLAKTARQIKIETADTPITID
                     LDQNQS"
     gene            complement(1322..>1509)
                     /locus_tag="PKZ53_23345"
     CDS             complement(1322..>1509)
                     /locus_tag="PKZ53_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNJ43440.1"
                     /translation="QQFRLREKGFPILRSQGQERGDQYVEVRITLPKVISEETKDLLR
                     HFAKLNPENPRAEIGLS"
BASE COUNT          362 a          349 c          351 g          447 t
ORIGIN      
        1 cttggtgctt ggttcttggt tcttggttct tggtgcttgg ttcttggttc ttggttcttg
       61 gttcctggaa ggtattgatt cctaagcacc aagcactaag taccaggtac taagcacatt
      121 cttcattctc cattcttcat cttggtgagg actatgtctc gaaaaaccag cgggagctct
      181 atggtacgga ttatctcagg ttcaagtgtt gtattgagtt tacatagccc tcgcgagaag
      241 atttggggta ttttggtttc aattgaacaa tctggggttc aaatctgcgg cgttgatatc
      301 aacacttttg acgattggct caattctgtc accaatggag agtcaaatat tggcttgacc
      361 aacagttttc tcccgatgtg gcgggtcgaa cgcatttctt tagatgaaac aattggggat
      421 attttgtcgc tggaagaaca atttgcgctc cgggtgggga tcacactcac cgaatacctg
      481 aagggcgcca aagatggata tgtgagttca actgaaaact ggcggcaata gacaagtcaa
      541 aacaaattga tttaaaccaa acaggctgac gatatcgggt ttgaggaaca tagattccaa
      601 ctcgaaatcg tcagcctgtt ttctgttgcc gctcttgttg agaaaagggc cacctcattg
      661 aagactggac caattcacga ttgattttgg tccagatcaa tcgtgattgg agtgtcagcc
      721 gtttcaattt tgatttgccg ggccgttttg gcaagcgtac tgcctcgaag ggtacggcgc
      781 tcggtttgtt caataatcac cagatggctg ggcggggttc gcaccagtgc gttttggaga
      841 tgttcctggt caaccgccat attttcagca atttgctcgc gcaggtattc taaaaaccga
      901 ttgaaatcac gttgcatttc gtccatgcgc tgccgcaaat caataatgat ttggactcca
      961 gccaggttga cccccatatc tcgggtcaaa ctcaaaatca tcttcagttt ttccaggtca
     1021 tcatcagtat agtaccgggt gtttttttct gaccgggacg gcgtcagaag accttcacgt
     1081 tcgtacagcc gaagcgtctg ctcgtgaatg ccaaacgtat tggccaccac accgatggtg
     1141 tatcgctttg gtcgcttctt ccgctcttgc gccacaacct acctccaaat tcaattgaat
     1201 gaagaatgaa gaatgaagaa atattagctc ttggttcttc ttcgaaagta ttgatttcta
     1261 agcactaagc accaaggcac caagcaccca tttcattctt cattctcaat tcttcattca
     1321 gttaggaaag tccaatttca gcccgagggt tttctggatt caactttgca aagtgtcgca
     1381 acaggtcctt ggtttcttca ctgatgacct ttggaagcgt aatccggacc tcgacatact
     1441 ggtctccgcg ttcctggccc tggctgcgaa gaatcggaaa tcctttttcc cgcaaccgga
     1501 actgctgcc
//
LOCUS       DAOQDO010001865         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96178_length_1508_cov_2.369580, whole genome shotgun sequence.
ACCESSION   DAOQDO010001865 DAOQDO010000000
VERSION     DAOQDO010001865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96178_length_1508_cov_2.369580"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKZ53_23350"
     CDS             <1..205
                     /locus_tag="PKZ53_23350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ43441.1"
                     /translation="TVELTPDNHQLVIRLEDDGLGFDPESFNHQTGRGLANIRSRASL
                     ISAQVSWIHKEPGGTVVEVRLNQ"
     gene            380..1069
                     /locus_tag="PKZ53_23355"
     CDS             380..1069
                     /locus_tag="PKZ53_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43442.1"
                     /translation="MKQDWKSPKVYLSISLAGILIVLLAGISFNFSLTDGFVRQVDSH
                     QPNSRKQQSSVESRGIQCSVSIRPDWWSKTTPAKIQVNLTNHSGKVIKLDGFVGLDLV
                     KSVNGKEIDHYTLEGQRNEFGSVMAISEAISVSGQQGREPEELQPNETKLIEIDPINS
                     AGWDRAISSTIRRENLFTVVPDGEYQLTVTILLRKIDDSVISGAAKKTNAKEDFLDVI
                     HKSKYKIIIGQ"
     gene            complement(1300..>1508)
                     /locus_tag="PKZ53_23360"
     CDS             complement(1300..>1508)
                     /locus_tag="PKZ53_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43443.1"
                     /translation="TMEDFKRDAKEEFLKTLTPEERLKGLPPEERLKGLPPEERFKGL
                     TAAEIQAYLEKLLTQSEPDSSEKP"
BASE COUNT          411 a          327 c          358 g          412 t
ORIGIN      
        1 gacggttgag ctgacgccag acaatcatca acttgtgatt cggctggaag atgatggcct
       61 cgggtttgac ccggagtctt tcaatcacca aaccggacgc ggcctggcca atatccgttc
      121 ccgcgccagt ttgatttcag cgcaggtctc ctggattcac aaagagccgg gtggaacagt
      181 ggttgaggtg aggctgaatc agtgagaaaa agatttcttc ctgggagcgc cggcatcctg
      241 ccggcaactg attggatatg aaccgtttgg tgccggcaag ataccggcgt ttccaggaaa
      301 tatttatctg aagaactata gcaacttatt tggctcagca agcaatactg tcattttggt
      361 aatggctgcg aggtgtaaaa tgaaacagga ctggaaatct ccgaaagttt acctgagtat
      421 ttcactcgcc ggaatactta tagttttact tgcaggcata tcatttaact tcagtttaac
      481 cgatggtttc gtcaggcaag tggattctca ccagccaaat tcacgtaaac agcaatcttc
      541 agtggagagt cgtggaatcc aatgttcagt gagtattcgc cctgattggt ggtcaaaaac
      601 aaccccagcg aaaatacagg tgaacctcac aaatcactca ggcaaagtca taaagcttga
      661 cggttttgtc ggactcgatt tagtgaaatc agtcaatggt aaagagatag atcattacac
      721 tttagaagga caaaggaatg aatttggatc tgtcatggca atttcagagg ccatctctgt
      781 atctggacaa caagggcgtg aacctgaaga acttcaaccc aatgaaacga aactgattga
      841 aattgatcca atcaactctg ccggatggga cagagctatt tcatcaacga ttcgacgaga
      901 aaaccttttt acagtcgttc ctgacggcga atatcagtta accgttacta ttttgctcag
      961 aaaaatagat gactccgtta tttcaggagc agcaaaaaag acaaacgcca aagaagattt
     1021 tttggatgtg attcataaaa gcaaatacaa aattatcatt ggtcaatgag acaatgatca
     1081 gtgattcttt gagacgttcc ttgggtgtca gagttttgag aaattcttcc tttgcatcgc
     1141 ctttgaaatc ttccatttct ttggagtacg gcccacttga cgccgctttg gtatttgcaa
     1201 ttcgatgctg gtcaccttca agcctgcctc aacccgcacc cgatgaaagt cgtgtcaagt
     1261 gggccgcaat ccaaaaaagg atctcaaccg ggttgtcttt cagggctttt cggatgaatc
     1321 tggctcagat tgcgtgagca atttttccag atatgcctgg atctcagcgg cggtgagccc
     1381 tttgaaacgc tcctcgggtg gcaacccttt gaggcgttct tcaggtggta atcctttgag
     1441 gcgttcttca ggtgtcaggg ttttgagaaa ttcttctttg gcatcgcgtt tgaaatcctc
     1501 catcgtgt
//
LOCUS       DAOQDO010001866         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96275_length_1507_cov_3.046832, whole genome shotgun sequence.
ACCESSION   DAOQDO010001866 DAOQDO010000000
VERSION     DAOQDO010001866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96275_length_1507_cov_3.046832"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..214)
                     /locus_tag="PKZ53_23365"
                     /pseudo
     CDS             complement(1..214)
                     /locus_tag="PKZ53_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008227983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            241..507
                     /locus_tag="PKZ53_23370"
     CDS             241..507
                     /locus_tag="PKZ53_23370"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNJ43444.1"
                     /translation="MVSHKVRRGETLAGVASRYGVSIQEVAQHNRMSSKAELRNGQVI
                     KVPVQKNEGSKYSVRSTKNSKHIAKSTPKGKASKGAKSSARRRR"
     gene            complement(634..1224)
                     /locus_tag="PKZ53_23375"
     CDS             complement(634..1224)
                     /locus_tag="PKZ53_23375"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNJ43445.1"
                     /translation="MPFELPALPFAKDALAPHISAQTFDFHHGKHHKAYVDNANKLIE
                     GTAHAEKSLEELIVAAWTDKNAGLFNNAAQIWNHTFFWNSLKPGGGGAPTGELADRIN
                     SAFGSLDGFKDEFKKAATTQFGSGWAWLVLDNGDLKVTKTPNAEPPIIHGQTALVTCD
                     VWEHAYYLDFQNRRPDFVQAFLDHLINWDFVAANLG"
BASE COUNT          342 a          412 c          401 g          352 t
ORIGIN      
        1 cgagatcacc gcggaagaac gacaccactg attccggaaa ggcaacgtcg accgccgggt
       61 cttcaaccgc cgcgcgcgtc aggccgctgg tcaccatcat caaggccatg tcgcccacca
      121 ccttggaact gggcgtgacc ttgacgatgt cgccgaacat gtcgttgacc tcggcatagg
      181 cccgcgccac ttccggccag tgttcgccca gccccggcca aatcctccta tcgcaccgag
      241 atggtcagcc acaaggttcg ccgtggcgaa accctggcgg gtgtggcatc acgctatggc
      301 gtttcaatcc aggaagtggc ccagcataac cggatgagtt ccaaagctga actgcgcaat
      361 ggacaggtca ttaaagtccc ggtgcagaag aatgaaggct caaagtactc ggttcgttcg
      421 accaagaaca gcaagcacat tgccaaaagc actccgaaag gaaaagcttc aaagggcgcc
      481 aaatcttcag ctcgccgccg tcggtaagcc gattttttca agttccaccc accgaaagca
      541 aaaaggtgag gcagaagttc attctgcctc acctttttcg ctttttcccg gcgtgcgatc
      601 tcgcaggcgg gtgaactgtt tccagggtct tttctagcct aaattcgcag ccacaaaatc
      661 ccagttaatc aggtgatcca aaaacgcctg aacaaaatcg gggcggcggt tctggaagtc
      721 cagatagtac gcgtgttccc aaacatcgca ggtcaccagc gccgtctggc catggataat
      781 cgggggttca gcatttggcg ttttggtgac tttcagatcg ccattgtcga gcaccagcca
      841 ggcccaaccg ctgccaaact gggtggtggc ggctttcttg aattcatctt tgaatccatc
      901 gaggcttcca aaggctgaat tgatccggtc agcgagttca ccagttgggg caccaccacc
      961 gccgggtttg aggctattcc agaaaaaggt gtgattccaa atttgggcgg cattgttaaa
     1021 caggccggcg tttttgtctg tccaggcagc aacaatcagt tcttccaatg acttttcagc
     1081 gtgtgcggtg ccttcaatca atttgttggc attgtccaca taggctttgt gatgcttccc
     1141 atggtgaaaa tcaaaggtct gggctgagat atggggcgct aatgcgtctt tggcaaaggg
     1201 taatgcaggc aattcgaatg gcattgactt tctcctcaga atgaagtgtg aaaaggttgt
     1261 ctcttgatgc cgctctttta taccaagtcc cggcggatgg ctatctgtct ttgcagaagg
     1321 atgattggag ctgaagacat cgggctgaag acatcgggct gaaaacatcg ggctcagggt
     1381 attaaacctg ttttcttcaa ccccgagctt gcgagtcttc agccccaagc ccgttttctt
     1441 cagccctggt ttttcacggt tgggcggctg ccgctccggc ctggctgcca taattggcca
     1501 gcgcttc
//
LOCUS       DAOQDO010001867         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96417_length_1506_cov_2.667815, whole genome shotgun sequence.
ACCESSION   DAOQDO010001867 DAOQDO010000000
VERSION     DAOQDO010001867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96417_length_1506_cov_2.667815"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..833
                     /locus_tag="PKZ53_23380"
     CDS             24..833
                     /locus_tag="PKZ53_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ43446.1"
                     /translation="MPDIQPWLNSLYAQLTGLGLKIIGAMVLWSIGRWLINIAVDLLM
                     RPLRKQPFDQTVFTWIGSTIRVLLNVTLIVAILGFFGVQTTTFAALLAAFGFAVGTAW
                     GGLLANFASGAFLIILHPFKVGDLISAGGVLGTVEEIGLFVTTINTLDNVKTFVGNNK
                     IFSDNIQNFSANPYRRVDLVAQINHSVDHKEAMALLKAGLAKIPNVVTAPPPDVEILS
                     FSDMGPVLAVRPYCHNDHYWQVYFDTNRLIRDSFGEAGFPAPQKHLTVHQS"
     gene            1219..>1506
                     /locus_tag="PKZ53_23385"
     CDS             1219..>1506
                     /locus_tag="PKZ53_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43447.1"
                     /translation="MSINPETLIVRREHLPERCEICHQSDQFDPVANHCSRCSLVKLS
                     PTSNPGHRDLKEFSNQRTTIEKIQVAGLGMIYSFIFAAGLGFLPLFVVVQLK"
BASE COUNT          372 a          365 c          349 g          420 t
ORIGIN      
        1 aaatttacct ggaggaaaat cacatgccgg atattcaacc ctggttaaat agcctgtatg
       61 cacaacttac gggtctggga ctcaaaatca tcggtgccat ggtgttatgg tccattggac
      121 gatggttgat caatatcgcc gttgatttgc tgatgcggcc actccgcaag cagccgtttg
      181 atcaaaccgt gttcacctgg attggttcaa ccattcgggt gttgctcaat gtcacattga
      241 ttgtcgccat cttaggcttt ttcggagttc aaaccaccac gtttgcggca ttgctggcgg
      301 ctttcggttt tgcggttgga acggcctggg gcgggttact ggccaacttt gcatcgggtg
      361 cttttttgat tattctgcac ccattcaagg tcggagacct gatttcagcc ggtggggtgc
      421 ttggaaccgt cgaagaaatc ggtttgtttg taacgaccat caacacgctc gacaatgtca
      481 aaacatttgt cggtaataac aaaatcttct cggataacat ccagaacttt tcagccaacc
      541 cctaccgtcg cgtagacctg gttgcccaaa tcaaccattc cgtagatcac aaagaagcaa
      601 tggccttgct caaagccggg ctggccaaaa tcccaaatgt cgtaactgca ccgccgccag
      661 atgttgaaat tctgagtttt tccgatatgg ggccggttct ggcggtgcgc ccctattgcc
      721 acaacgatca ttactggcag gtatattttg acaccaaccg gctgattcgc gattcatttg
      781 gtgaagccgg cttcccagcg ccacaaaaac acctcacggt ccatcaatca taaccttcct
      841 tgcttctctt ttcggagtgc ggtggcttga caccgctttg gtattgccag gttcatccgc
      901 cgcactctga aaaactgtag tcccttgaaa aagtgtcaat gcgttgacag gcatttatag
      961 ctctgaatct gaacaagtta agtcgggaaa cttaggtgag caagtagttg ctgatcttca
     1021 ctgactcagg attatttttt tcagctactg tcagtcagaa acaccatgtg ccatcggttt
     1081 gtggtagttc acaaattgag acaaaccttc ctcattacac acgatcagaa tgccaggatg
     1141 gcaagtcgtt tgaatgtgtc aacgcgttga cagtcattga acttgctgat tctgaaatag
     1201 ttaagtgggg aaactcttat gagcatcaac ccggaaactc tcatcgtcag gcgtgagcac
     1261 ttgccggaac gctgcgaaat ctgccatcag tcagaccagt ttgacccggt ggcaaaccac
     1321 tgttcacggt gtagtttggt taaactctcc ccaacctcca acccaggtca tagagatcta
     1381 aaagagtttt caaatcaacg aacaactatt gaaaagattc aagtcgcagg tttgggaatg
     1441 atctatagtt tcatctttgc ggctggtttg gggttccttc ctttatttgt cgttgttcag
     1501 ctaaaa
//
LOCUS       DAOQDO010001868         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96472_length_1506_cov_1.760855, whole genome shotgun sequence.
ACCESSION   DAOQDO010001868 DAOQDO010000000
VERSION     DAOQDO010001868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96472_length_1506_cov_1.760855"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..665)
                     /locus_tag="PKZ53_23390"
     CDS             complement(54..665)
                     /locus_tag="PKZ53_23390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNJ43448.1"
                     /translation="MSILNQNQPVVIKFTQDQTYVRQYYNLRERMYTSVWGLQGFNGQ
                     EDEFDQKSHILVAVKDGRVVGGARISVRTPQAPNLLPMEKREVSLEHQIADLNLPQCR
                     YGEYSRLALLPEYRGGGCTLAMYKALNRKSISLNLKFAFALSPLTQARSYRTIYQALG
                     FNYTILEDVVIPENPEYEGIKMCLSVLDLSRYCSDSVDFVEEL"
     gene            complement(891..1250)
                     /locus_tag="PKZ53_23395"
     CDS             complement(891..1250)
                     /locus_tag="PKZ53_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43449.1"
                     /translation="MSISQQEKARDWVNKNTGAAVAVVLATALVPGAAAAALFAQEVA
                     MAYKIGGIYKADFSMHDAKAVAAHIGLGALAGKIAALEAAILTGPLAFAIKPIIAAGI
                     VKAIGELVIAYCEEKWG"
BASE COUNT          381 a          374 c          352 g          399 t
ORIGIN      
        1 gaaaaggctg ccgtggcata agggtcccct tgagtatcca ggtcattgac gatctataat
       61 tcctcgacaa agtctaccga atcagagcag tatcgggata aatccagaac cgagaggcac
      121 attttaattc cttcatattc tggattctca ggtatcacca cgtcttccag gattgtgtaa
      181 ttaaatccca gtgcttgata gattgttcgg tagcttcggg cctgtgtgag tggcgagagg
      241 gcaaatgcaa atttcagatt aagcgaaata cttttccgat tcagtgcctt gtacattgcc
      301 agggtgcagc cgccacctcg atattctggg agcaatgcca gtcgagaata ttcgccatac
      361 ctgcactgtg gcaaattgag atcagcgatc tgatgttcca gactaacctc tcttttttcc
      421 attggtaaaa ggttgggggc ttgaggtgtt cgaactgaga tccgtgcacc acctaccacc
      481 ctcccatctt ttaccgcaac gaggatatgg cttttctggt caaattcatc ttcctggcca
      541 ttaaatccct gaagtcccca gacgctggta tacatacgct cccgtaagtt gtaatactga
      601 cgtacgtagg tttgatcttg ggtaaattta ataacaactg gctggttttg attcaggatt
      661 gacatatttg taaacctctg gctggtaatt tggttagcga gtgattcaac aggttttaat
      721 cttcatccag gactggccta tcacaaatgc aacttcacct ggctcatctg gcctgttcta
      781 aacaaaggat gacgactccc tggtaccaca ccgccacaac ttaaaggatt tttgagagag
      841 accagactaa attggagttc accaaaagca ccgctggtca tctgttcaac tcatccccat
      901 ttttcttcgc agtaggcaat aaccaattct ccaatcgcct tgacgatacc tgcggcaata
      961 atcggtttaa tggcaaatgc aagtggaccg gtgagaattg cagcttcgag agctgcgatt
     1021 tttccagcca gagcccccag tccaatatgg gctgcgacgg ctttggcatc gtgcatggaa
     1081 aaatctgctt tgtaaattcc tccaatctta taagccatag ccacctcctg ggcaaagagg
     1141 gctgcggctg cggccccggg taccaatgcg gttgctaaaa caacagccac ggcggcgcca
     1201 gtatttttgt ttacccaatc cctggctttc tcttgttgag agattgacat gtttaacctc
     1261 cttttcaggc aatcagtaaa agaacagaac tcccgcgtgg gcacgctgcc cagggtgagt
     1321 cttcgagttc tggaaatctg ggcatcccca cttgccactc atccggcaag ccagagtgag
     1381 gctgtgagct gacgactgat gacaaactgg tatcgggttg attggatcgc tgtcattgaa
     1441 gcgacacccg caccaccggc aagcgaagta cacactgaaa actggtgggt gtcagccaaa
     1501 gttgtt
//
LOCUS       DAOQDO010001869         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96498_length_1505_cov_4.789655, whole genome shotgun sequence.
ACCESSION   DAOQDO010001869 DAOQDO010000000
VERSION     DAOQDO010001869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96498_length_1505_cov_4.789655"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..963)
                     /locus_tag="PKZ53_23400"
     CDS             complement(82..963)
                     /locus_tag="PKZ53_23400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43450.1"
                     /translation="MAYTLISRLVLIVLTIFLLVACGVEPAAAQNSLVGQWVSETEQM
                     KMTILLLPDGRYSAEIVVENTPVRETGRYVVRGTTLRFEPEGDTPGEYLVSLRGTQMT
                     LTGTNATLVFEKVSGTTDTVEPPTKQADTTQLSEDDQWRARIRIAKMTSQPPQVPVGE
                     VPADPNPRRIFSQPTVFTNQQLYYWMTAFPIVVKDHPEWGELRSTMKYYFFTNGRFFF
                     KSVQYHHTYTTREGNVTALWGRYHVQEDEQVQLESDAGEKLILPLKYGRRNLIFGETV
                     LGQIDWENEALQRYLNK"
     gene            complement(1012..>1505)
                     /locus_tag="PKZ53_23405"
     CDS             complement(1012..>1505)
                     /locus_tag="PKZ53_23405"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNJ43451.1"
                     /translation="ILHSSFSILNSSFSILHSKMTDKVSDQASFRIARISRKIMSHYE
                     RRCRDVGVTPVQLLVLSALNHRNASTIGELADELGFDSTTMTRFIQRMIELKLVERIA
                     DPADRRVQRVYLHKEAKKRLKSWLAAAEKIETELFAQLNEADRTALELTLATLEHLEF
                     KPT"
BASE COUNT          344 a          364 c          379 g          418 t
ORIGIN      
        1 gtttcttcat tcttcattct tcattcttca ttcttcattc tcaattcttc attcttcatt
       61 cttcattctt cattcttcat tctatttgtt caaatatcgc tgcagggctt cattttccca
      121 gtcaatctgt cccagtactg tttccccaaa gatcaaattc cggcgcccat atttcagtgg
      181 aaggatgagt ttttcacccg catcgctttc aagctggacc tgttcgtctt cctgcacgtg
      241 atagcgtccc caaagtgcgg tgacgttgcc ctcacgagtc gtgtaggtgt ggtgatactg
      301 gactgattta aagaaaaatc ggccattggt aaaaaagtaa tacttcattg tgctgcgaag
      361 ctcgccccat tctggatggt ccttcaccac aatcggaaaa gccgtcatcc agtagtaaag
      421 ctgttgattg gtgaaaacag ttggctgact gaagatccga cgcggattag ggtcagcggg
      481 gacttcgcca actggaacct gggggggctg gctggtcatt ttggcaatgc ggatgcgtgc
      541 ccgccattga tcatcttccg acaactgggt ggtgtctgcc tgtttggtcg ggggttcaac
      601 cgtatctgtc gtgcctgata ctttttcgaa gacgagcgtg gcattggtgc cggttaaggt
      661 catctgtgtt ccacgcagtg aaacaagata ttcacccggc gtgtcgcctt ccggctcaaa
      721 gcggagcgtt gtcccacgaa ccacataccg gcctgtttca cgcaccggag tgttttcgac
      781 cacgatttca gcactgtacc ggccatccgg taacaaaaga atggtcattt tcatctgttc
      841 ggtttctgaa acccattgtc cgaccagact attttgcgcg gcggctggtt cgacaccaca
      901 ggcaaccagc aaaaatatgg tgagcactat cagcacaagg cgggaaatga gcgtgtaagc
      961 cataatcttc tccgattggg actgagccaa tcagaatgga agctggctca gttaagtagg
     1021 tttaaattca aggtgttcca gcgtggccag tgtcagttcc agggccgttc ggtcagcttc
     1081 gttcaactgg gcaaacagtt cggtttcaat tttctctgcc gccgcaagcc acgatttgag
     1141 ccgttttttg gcttctttgt gcagatagac gcgttgtacc cgacggtctg ccggatccgc
     1201 aatgcgctcg accagtttga gttcgatcat gcgctggata aaccgggtca tggtggttga
     1261 gtcaaaccca agctcgtcgg ccaattcacc aattgtggat gcattgcggt ggttgagcgc
     1321 actgagcacc agaagctgga ctggggtcac tcctacatcg cgacaccggc gctcataatg
     1381 gctcatgatt tttcgagaaa ttcgcgcaat gcgaaatgaa gcttgatctg agactttgtc
     1441 agtcattttt gaatgaagaa ttgagaatga agaattgaga attgagaatg aagaatgaag
     1501 aatga
//
LOCUS       DAOQDO010001870         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96525_length_1505_cov_2.855862, whole genome shotgun sequence.
ACCESSION   DAOQDO010001870 DAOQDO010000000
VERSION     DAOQDO010001870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96525_length_1505_cov_2.855862"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="PKZ53_23410"
     CDS             <1..1465
                     /locus_tag="PKZ53_23410"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ43452.1"
                     /translation="SSHYLHACTYTACGHSVLLSGSIPAITGIIGNEWVDRESGFKVT
                     SVSDADYVGVPEGRGASPRKMMSTTLGDQLRLQNNFRSRAIGISLKDRSAILPAGKSA
                     NAAYWFDTAKGQMQTSSYYLPQLPDWVKTFNDQKIPTSYFGKTWDRVLPESEYARSDQ
                     DNAPYETGLSGVKTFPHVVNGGGTQAGPKFYDDFTRTPWANDYLVDFAELAIENEKLG
                     QDEYTDVLSVSFSANDIMGHAFGPYSQEMQDMVIRTDRTLERFFQIIDQRVGLANTLV
                     VFSADHGVAPVPEYAQAKKLGGGRVSKKALYDIISQTLNGKYGEAPWIAGSSNESIYL
                     NQEVIKSKGLNRSEVERVAGEAAMNIQGVATYFTRTQLINGPQPASDLSQRVAAAFNP
                     QRSGDVYLIAQPYFFFEEDYFLATTHGSPYSYDTHVPVILMGKELRPGQYPAPSSPAD
                     IAPPLAAILGIEPPSGSVGRVLTEALEPAVAPTKKKK"
BASE COUNT          374 a          395 c          389 g          347 t
ORIGIN      
        1 gtcatcgcat tacctgcatg cctgtaccta caccgcctgt ggacattcag ttttgctttc
       61 tgggtcaatc ccggccatta caggaattat tgggaacgag tgggtagatc gcgaatcagg
      121 ctttaaggtc accagcgtga gtgatgctga ttatgttggt gtccccgaag gtcgcggtgc
      181 cagcccacgg aaaatgatga gcaccaccct tggggaccaa cttcgcctgc agaacaattt
      241 tcggtcgcgg gccattggga tttccctcaa agatcggagc gcgattcttc cggctggaaa
      301 aagcgccaac gcggcctact ggtttgacac ggccaaaggg cagatgcaga ccagcagcta
      361 ttatcttccc caacttccag actgggtcaa aaccttcaat gatcagaaaa tcccaaccag
      421 ttattttggg aaaacctggg accgggtgct tccagaaagt gaatatgcac gctcggacca
      481 ggacaatgca ccctatgaaa ccgggctttc tggagtgaaa acttttccac atgtggtgaa
      541 tggcggtggc acccaggctg gtcccaaatt ttatgatgat ttcacccgga ccccctgggc
      601 caatgactat ctggtggatt ttgccgagct ggcaattgaa aatgagaagc tgggacagga
      661 tgaatacaca gatgtcctct cagtcagttt ttcagccaat gacatcatgg gccatgcatt
      721 tggaccgtat agccaggaaa tgcaggacat ggtgatccgc accgaccgca cccttgaacg
      781 gttttttcaa attattgacc agcgggttgg gctggccaat actctggtag ttttttcggc
      841 tgatcacggc gtggcgccgg tcccagaata tgcgcaggcc aaaaaactcg gtggcggacg
      901 ggtctcgaaa aaagccctct atgacatcat cagccagaca ctcaacggaa aatatggtga
      961 agcaccctgg attgccggta gttccaacga atcaatctac ctcaatcagg aagtgatcaa
     1021 atcaaagggg ttgaatcggt cagaggttga gcgtgtggca ggtgaggcag cgatgaatat
     1081 tcaaggggta gcgacctact ttacccgaac ccagttaatt aacggtccac aacccgccag
     1141 tgacctctcg cagcgagttg ctgctgcgtt taacccacaa cgaagcggtg atgtctatct
     1201 gattgcccaa ccctactttt tctttgaaga agactacttt ctggcgacca cgcacgggtc
     1261 tccatacagt tatgacaccc acgttcccgt cattttgatg ggaaaagagc tacgtcctgg
     1321 tcaatatccg gcgccgagtt ccccggctga tattgcgcca cccctggccg caatccttgg
     1381 gattgaaccg ccgagtggtt cagtcggtcg ggtgttaacc gaagcgctgg aaccagccgt
     1441 cgccccaacg aaaaagaaaa aataaagtct ttcggatttc ccgggttcct agccggcatt
     1501 ttcac
//
LOCUS       DAOQDO010001871         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96757_length_1503_cov_2.509669, whole genome shotgun sequence.
ACCESSION   DAOQDO010001871 DAOQDO010000000
VERSION     DAOQDO010001871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96757_length_1503_cov_2.509669"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKZ53_23415"
     CDS             complement(<1..436)
                     /locus_tag="PKZ53_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43453.1"
                     /translation="MKFEFYHSGLESVPKLSVDGTVPSSLHFSHWKGNQTPRELKADT
                     STEICLNLVSHPDRLRFTQGIELITNNHFDADGLLSVWVTLTGPPALQHRDLLIATAE
                     AGDFSEYSTDEAVRLCILLQGGDQVILDEPTLSPLAQHLAGKP"
     gene            519..594
                     /locus_tag="PKZ53_23420"
     tRNA            519..594
                     /locus_tag="PKZ53_23420"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:552..554,aa:Phe,seq:gaa)
BASE COUNT          380 a          345 c          372 g          406 t
ORIGIN      
        1 ctggttttcc agccagatgc tgggccagcg gagacagcgt tggctcatcc aggatcacct
       61 ggtctccacc ctggagcaaa atgcagagcc gaaccgcctc atcggttgaa tactccgaaa
      121 aatcgcccgc ttcagccgtc gcaatcagga ggtcacgatg ttggagcgcc ggtgggccgg
      181 tgagcgtgac ccagacagaa agcagcccgt cggcgtcaaa gtgattattg gtaatgagtt
      241 caatgccttg cgtaaaccgg agccgatcag gatggctgac aaggttaaga cagatttcgg
      301 tcgaagtatc ggctttgagt tcgcgaggag tttgattgcc cttccaatgc gagaaatgca
      361 ggctactggg aaccgtccca tcaaccgaaa gtttgggtac tgactccagt ccggagtgat
      421 aaaattcgaa tttcatatgg gtgcgggcct cgaaaaaagg aattgacagc gccaaagcgg
      481 ttgcgtatta tccgcgttct gtcttgttca ttcaatgcgg ccaggtagct cagtcggtag
      541 agcagcggac tgaaaatccg cgtgtcggcg gttcgattcc gtccctggcc accatttacg
      601 agaaacaaaa aagcggcgag cctcacacga ctcgccgctt ttttgttttt tcaaatggag
      661 tcgtctgtaa aaccagcgaa attcagctat ctgatggtga taccattttg gaatgaaatc
      721 ctcgtattat gggaaggcta aatgattgaa aaaattgtat ttccctgaac cctgaacccg
      781 ataccctgac ccctaattgg tataacctca ttcccacggt accagtttgg cgctaaccaa
      841 actggcaaca tccctgggct gaagcagtca gaggacacct tcgacggctt cagcccagtc
      901 tgttttggtc gattcttttc cttacttaat tgcagcgtca ggaaatgtca ggttgtgata
      961 tattgtccga attggaagct cacaattgat tgttatacca gtttgtagtc agtagcccgt
     1021 agtcagtagt tcactaagtc tatttgaatg aattgtttga ctgttttcta atacgaggct
     1081 tttattccaa aatggtatta ccagagaaag aatagagtct ggatgatttg tttctatcat
     1141 cacccattga tctggcgatt gcttcacaaa atgctcaacg tgacattttt cctgcgaaat
     1201 cagcaggtat tcgcgaaacg tctcaatcat tcggagcatt tcgaatttct caccgcgatc
     1261 ataatttttg gttgagtgtg aaagcacttc gatcaataga actggattca gtcaggtatt
     1321 cctggttgaa aaaacgggga atcagattgg ttgacatatt ttaagtctcc caaaaacggc
     1381 ttgatgcctg gatgggggtt gctcagtgtg tcatttgaac ttgaatcagg tcattttgca
     1441 cctgagccgc aaccaccggc tttcgacgcg ctttgatttc tatgccggat ggtggaccga
     1501 cgc
//
LOCUS       DAOQDO010001872         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96814_length_1502_cov_14.952315, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001872 DAOQDO010000000
VERSION     DAOQDO010001872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96814_length_1502_cov_14.952315"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..895
                     /locus_tag="PKZ53_23425"
     CDS             365..895
                     /locus_tag="PKZ53_23425"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ43454.1"
                     /translation="MKFLSHSAKLFFIAALAFFLPTVFMTMPNTTDPVQAAQAKAAPA
                     FSLDNVNGGKVSLASYRGKVVVLNFWATWCGPCIAELPDFKAISSEFGPKGVQVIGLS
                     IDGGPDVVKTFLQKNPLNYVVAMSTGKVSSSYGVTGSIPQTFIIDRKGKLRKSYTTRI
                     NKQQVVADINALLAEK"
     gene            complement(1083..>1502)
                     /locus_tag="PKZ53_23430"
     CDS             complement(1083..>1502)
                     /locus_tag="PKZ53_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43455.1"
                     /translation="FSRDSFENLLFGICRFREYTGKYPHRISVVSWKFKQERFDLHRQ
                     AIRFPADRFEFIGVNNPPDLESALIGEHQNSLIPFSQDPFGTGPVLAAKRHHRNPFHR
                     QHGYTTSCPEVALLLNDAGPDPFPDKLPWEKHNGHAT"
BASE COUNT          340 a          357 c          391 g          414 t
ORIGIN      
        1 cctgccacgc ggggatcaag tccaaaaggc cctgggccgc tctggttcga ttgttgcggt
       61 ggctgatatg ggtattgtga ttgttgctgc tgttgctgat aagggtcata aggtgggtag
      121 ttcgacattc gggcaaagtc ctccgtttga aaaggggtta accgtgtgaa tatcctgatt
      181 caaaaacgtg tttaaaagtg agtgtcaaca aagctgtgag gagaagtgat attgaactat
      241 tcctggattg caaaacattt gtcaaatcca cagaagcctt gagttccgaa aagcatcctg
      301 tggtactctg cagacaactg attcaatcac tttactttac aatttacaat ttggagcctt
      361 ttcaatgaaa tttctgtctc attcagccaa actttttttc atcgcggcgc ttgccttttt
      421 ccttccaact gtgttcatga ccatgcccaa cacgaccgat ccagtgcagg ctgcccaggc
      481 caaagcggcg ccggcttttt ctctggacaa cgtcaatggc ggcaaagttt cactggccag
      541 ctaccgtggc aaagtggtgg tgctcaattt ctgggcaacc tggtgcggcc catgtattgc
      601 cgaattacct gatttcaaag ccattagcag cgaatttggt cccaaaggcg tgcaggtgat
      661 tggtctttcg attgacggcg gcccagatgt cgtcaagaca tttctccaaa agaacccatt
      721 gaactatgtg gtggccatga gtaccggcaa agtttcatca agctatggcg tgactggttc
      781 cattcctcag acgtttatca ttgaccgcaa aggcaaactc cggaaatcct ataccacgcg
      841 gatcaataaa cagcaggtgg tggctgacat caatgccttg ctggccgaga agtaagacta
      901 tttagggttc cgggttccgg gttccgggtt ccgggttccg ggttccgggt tccgggttcc
      961 gggttttcga aatccttttt cgtccttttt tgtccctgtt gtccttttcg tcctttttgt
     1021 cccttttgtc ttttccctaa accctaaacc ctaaaccctg atctccccaa cgggcacgtc
     1081 actcaggtgg cgtgcccatt gtgtttttcc cagggaagtt tgtctggaaa tgggtctggt
     1141 ccggcatcgt ttaggagcaa ggccacttcc gggcagcttg tggtgtagcc gtgctggcgg
     1201 tgaaatgggt tgcgatgatg acgtttggcg gccagcaccg ggccggtgcc aaagggatcc
     1261 tgggaaaaag gaatcaggct gttttggtgt tcgccgatca gggctgattc caggtcaggt
     1321 ggattgttga caccgatgaa ttcaaatctg tctgcgggaa accgaattgc ctgccggtga
     1381 agatcaaatc gctcctgttt aaacttccaa ctgaccaccg aaatccggtg tggatacttg
     1441 cctgtatatt cacgaaagcg acagatacca aagagaaggt tttcgaagga atctcgtgaa
     1501 aa
//
LOCUS       DAOQDO010001873         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96835_length_1502_cov_3.211472, whole genome shotgun sequence.
ACCESSION   DAOQDO010001873 DAOQDO010000000
VERSION     DAOQDO010001873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96835_length_1502_cov_3.211472"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..>1502)
                     /locus_tag="PKZ53_23435"
     CDS             complement(417..>1502)
                     /locus_tag="PKZ53_23435"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43456.1"
                     /translation="GSVALFRDRVFQLPASPVSMFTTGMLSLAEKFEAIRFLTRLEHI
                     KTEQLLETSIQTWVDQHLKHATMREYLKALLRLSTYSNAPEQTSAGVALAQMKQGVKH
                     NVLYLHNGWQSMVNSLCEMATQAGVEIRTSTPVQSVIRDEQGTVRGVRLITGEQLSAA
                     AVILVVPPKVALDLVEGSHQSAFGRWAAQTTPIKLACLDLALSRVPQPKTNFALGIDQ
                     PWYFSVHSAAAKLGPTGGGLIHVAKYLPINTSNSSASVRAELEHVLDLVQPGWREVIV
                     HQRFLPEMTVANSNPRPVASGLGDRPQTAVAEIPGLFLAGDWVGPEGLLVDASVASAR
                     DAARKATEFISKNDLSFLVLKEVSSNN"
BASE COUNT          378 a          376 c          379 g          369 t
ORIGIN      
        1 cgtagcgagg gcaatccgtg gttcaaacca tgaagccaat gaaaaagtaa acaacaatcc
       61 tggagtcagc accaatgaag ccagaaacag gtgacgcata acattcatat ccccaaaagt
      121 cagtcgtgag ttgtcggtta tatccaatct tatgagatat ttagcttgtt atctggcaga
      181 aagaatccgt ctcaattcgt cattttcaga gaaaaggcca ggcgactcgt agatgaaatc
      241 tgacgtgaga aagggctcct tgatgattga agagtaacca acccgcaaaa cgagttgggt
      301 tgtctgggca taaacccggc aatggtacca ctgaccaaat aaaacctgcc tgctcgacaa
      361 tcaggctccc caccccctca gttgtcaaga ttttttcagc aggtttcttt ctttttttag
      421 ttgttggatg aaacttcttt aagtaccaga aaagaaaggt cattttttga gataaattcc
      481 gtggctttgc gtgctgcatc tctggcgctg gccacgctgg catcaaccag caacccttct
      541 ggaccaaccc aatctccggc taaaaacaat cctggaattt ctgcaactgc tgtttggggg
      601 cgatccccga gacctgaagc aactggcctc gggttgctat tcgcgacggt catttctggc
      661 aaaaatcgct ggtggacaat cacttcgcgc caacccggct ggaccagatc caacacgtgt
      721 tcgagttcgg cgcgcactga agctgatgaa ttactggtat tgatcggaag gtatttggcc
      781 acatggatga gtcctccacc agtcggaccc agtttggcgg ctgccgaatg aaccgaaaaa
      841 taccagggct ggtcaatccc caatgcaaaa ttggttttgg gctgtggcac ccggctcaga
      901 gccaggtcaa ggcaggcgag tttgatcggc gtggtctgtg ctgcccagcg gccaaaggct
      961 gactgatgac tgccttcgac cagatccagt gcgacttttg gaggtactac cagaatcaca
     1021 gcggctgccg agagttgttc accggtgatc agtcgaactc cgcgaacggt tccctgttca
     1081 tcacggatga cagattgcac cggggtgctg gtgcgaattt caaccccagc ctgggttgcc
     1141 atttcacaca gactgtttac cattgattgc cagccgttat gcaaatacaa gacgttatgt
     1201 tttacaccct gtttcatttg ggccagagcc accccggcac tggtttgctc cggagcattg
     1261 gaataggtcg agaggcgcag gagtgccttg agatattcgc gcattgtggc gtgtttcaaa
     1321 tgctggtcaa cccaggtttg aattgaagtt tccagcaact gttctgtctt gatgtgctct
     1381 aatctggtca gaaatcgaat agcttcaaac ttttctgcca ggctcaacat tccggttgtg
     1441 aacatggata ccgggctggc cggaagttga aagacccgat cccggaaaag cgcaactgaa
     1501 cc
//
LOCUS       DAOQDO010001874         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96958_length_1501_cov_3.570539, whole genome shotgun sequence.
ACCESSION   DAOQDO010001874 DAOQDO010000000
VERSION     DAOQDO010001874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96958_length_1501_cov_3.570539"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PKZ53_23440"
     CDS             complement(<1..1229)
                     /locus_tag="PKZ53_23440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43457.1"
                     /translation="MIGTYLLHYKILKKLGEGGQGEVYQAEDTRLRRTVAIKILPPEL
                     VADEKSRKRFLREAQLASALDHPNICTVYEINEDKNLHFIVMQYLEGKRLKQMVRGRP
                     LELESVLSIAIQLADALAAAHERGVIHRDVKATNVIVNDRGQAKILDFGLAKATTTDI
                     GDQSVMELTQAGVPFGTAGYMSPEQARGGTIDQRSDVFSLGVLIYEMVTGKLPFQGKS
                     SVEIMHAVMHEAAPPLAQTNPNLPDRLQVILDKAMAKGASGRYQQMRDFQADLKQLMR
                     NVQSQSGGMVDESIMPPIAPRHEKPGWLKNGMVGRLLNRLMSPGEQPPPRPSSSRERD
                     SVGTGTQPGSPRPDPGATLPPGPPPREKDSLEPSQEQRQARSLRDSMPSTWKNNEKRA
                     IAILPFKNLSGDPESDF"
BASE COUNT          327 a          383 c          427 g          364 t
ORIGIN      
        1 taaaaatcgg attctgggtc accgctcagg tttttgaacg gcaaaatcgc aatggcccgc
       61 ttttcattgt tcttccaggt actgggcatt gaatcacgca ggctgcgagc ctggcgttgt
      121 tcctgagaag gttcaagtga atctttttcc cgtggtggcg gtccaggcgg aagcgtggca
      181 ccaggatcgg gacgaggtga cccaggttgg gttccagtac caactgaatc acgttcacgc
      241 gatgatgaag gacgcggtgg cggctgctca ccaggagaca tcaatcggtt gagtaaccgg
      301 ccaaccatcc cgtttttgag ccagccgggc ttttcatgac gtggcgcaat gggtggcata
      361 attgattcat caaccatccc tccggattgc gactggacgt ttcgcatcaa ctgtttcaaa
      421 tccgcctgga agtcccgcat ctgctgatag cgtccggatg cccccttggc catggcctta
      481 tccagaatga cctgcaatcg gtcgggcaga ttggggttgg tttgagccag gggcggtgcc
      541 gcctcatgca tcacagcatg cataatttcg acactgctct tgccttgaaa cggcaatttt
      601 ccggtcacca tttcatagat taagaccccg agcgaaaaca catctgagcg ttgatcaatg
      661 gtgcccccac gagcctgttc gggtgacata tagccagccg tgccaaaggg caccccggcc
      721 tgggtcaatt ccatcactga ctggtcacca atatcggtgg tggtggcttt cgccaatcca
      781 aaatcgagaa tttttgcctg cccgcgatca ttgacgatca cattggtggc tttgacatcc
      841 cggtgaatga cccctcgttc gtgggctgcc gccagggcgt cggccagttg aattgcaatc
      901 gaaagcacgc tctccagctc caatggtcgg ccacggacca tttgcttgag gcgcttccct
      961 tccaggtatt gcatcacaat aaagtgcagg tttttgtctt cgttgatttc gtagacggtg
     1021 cagatattgg ggtgatcaag tgcggaggca agctgggctt ctcgtaaaaa ccgtttgcgc
     1081 gatttttcgt cggcgacgag ctcaggtggg agaatcttga tcgcgacagt ccggcggagg
     1141 cgggtgtctt cggcctggta aacttcaccc tggccaccct cgccaagttt tttgagtatt
     1201 ttataatgga gaagataggt gccaatcata caagtcggtg ggcggatttc cgctgagata
     1261 agacctggga atcgtgcggg ccaagaacca atgggcagcg ctgccttgga aaactcccaa
     1321 cggtctgagg gtgggctcaa tcgtcgggga tctgactgga atggtcagcc agcggattga
     1381 caaattcgat actagaccat tttcttgtgt gagggaaggg caaagacaac aatagcttgg
     1441 gttgtttttg tccagtcagg gccgaaagga ccgggagcaa acccattttg acccggctat
     1501 a
//
LOCUS       DAOQDO010001875         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_96993_length_1501_cov_2.533887, whole genome shotgun sequence.
ACCESSION   DAOQDO010001875 DAOQDO010000000
VERSION     DAOQDO010001875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96993_length_1501_cov_2.533887"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PKZ53_23445"
     CDS             <1..1200
                     /locus_tag="PKZ53_23445"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43458.1"
                     /translation="DVKTQKYGELRYVVYVVTRPSGKTHRQGEGPDSSTAPIQWTDLG
                     PAAPIDQLANEFRQALRKKSPLEKVGDSKGTGAQRPVLEIARELDQLVMQPVRKLVGN
                     ARHLLMSPEGELNLIPFAALVNEQGSFLVEHYTLTYLTSGRDLLRLQVKIAPAQPPLI
                     VADPDYAEGGGPTLFGTHLPPLKRLLGTKVEARMIQTEFPDAEVKLGPAAGKMALEAV
                     EKPELLHIATHGYFMGGGNALVAVADNQRALAKDDEQPINIDQIKRENPFLRSVLFFA
                     DANRSEQGTMTALEVAQLNLWGTKLVALSACDTGVGEIKNGDGVYGLRRALVLAGSEA
                     QMISLWAVSDSGTQELMANYYKRLKAGEGRSEALRNVQLKMLKNPRRRHPFYWASFIQ
                     SGEWKPL"
BASE COUNT          361 a          372 c          423 g          345 t
ORIGIN      
        1 gatgtcaaaa cccaaaaata tggggaattg cggtacgtgg tttatgtggt taccaggcca
       61 tcaggaaaaa ctcaccggca gggcgaaggt ccagactcgt caaccgctcc aatccagtgg
      121 actgatttgg gaccagccgc gccaattgac caactggcca atgaatttcg gcaggcattg
      181 cgcaagaaat ctccacttga aaaggtagga gattccaaag gtacaggtgc ccaacgacca
      241 gtcctggaaa tcgctcggga actggatcag ttggtgatgc aaccggttcg caagctggtg
      301 ggcaatgcca gacacctgct gatgtcaccc gagggtgaac tgaatctgat tccctttgcg
      361 gctctggtca atgaacaagg ttcgttcctg gtcgagcact ataccttgac ctatctcacc
      421 agtgggcgtg atttgctgcg attacaggtg aaaattgcgc ctgcccagcc acctttgatc
      481 gtggccgatc cagattatgc tgaaggcgga gggccgacat tgtttggcac acacctgcca
      541 ccattgaaaa gacttttggg gacaaaagtt gaagcccgga tgatccagac tgaatttcct
      601 gatgccgagg tgaaacttgg tccggctgcc gggaaaatgg cgttggaagc ggtcgagaaa
      661 cctgaactcc ttcacatcgc gacccatgga tactttatgg gcggcggaaa tgctctggtc
      721 gcagtagctg ataatcaacg tgcgcttgcc aaggacgacg agcaaccgat caacatagac
      781 cagatcaagc gggaaaatcc gtttctgcgg tcggttcttt tctttgctga tgccaatcgg
      841 agtgagcagg gaaccatgac ggcactggaa gtcgctcaat tgaatctgtg gggcaccaaa
      901 ctggtcgcat tgtcggcctg tgacaccggt gtgggagaga taaaaaacgg cgatggggtg
      961 tatggcttgc ggcgggcgct ggtcctggcc gggagtgaag cccaaatgat cagtctatgg
     1021 gcggtttctg acagtgggac ccaggaattg atggctaact attacaaacg gctcaaagcc
     1081 ggtgaaggtc gttctgaagc cctgcgcaac gttcagctca aaatgctcaa aaacccccga
     1141 cgccgccacc cgttttattg ggcttctttt atccaatccg gtgaatggaa accgttgtga
     1201 ttccagtttg gcatcagaag tcatgcgatt tttggctgaa ggtatcgggc cgtaagcgcc
     1261 aggataaggg aaaccaatgg tttgcgctcc agcaggagcc gccagagggt agcctggggg
     1321 gcaaagctac cccaggctac cctctggcca cctcttcgag gtgtattctt gtgcctgcta
     1381 tgggtgtccc gacctgggcg ggactgattt ttgagccagg tggtgctatt ttgggcaaaa
     1441 ttgaggtcat tctcaattct tcattctcaa ttcttcattc cccaaatttc gcccaaaata
     1501 c
//
LOCUS       DAOQDO010001876         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97106_length_1500_cov_2.858824, whole genome shotgun sequence.
ACCESSION   DAOQDO010001876 DAOQDO010000000
VERSION     DAOQDO010001876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97106_length_1500_cov_2.858824"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1056
                     /locus_tag="PKZ53_23450"
     CDS             316..1056
                     /locus_tag="PKZ53_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43459.1"
                     /translation="MKSNSVSHSPLFEGVAINLSRNPFRNRRLFWLGWLIGIAVLLVV
                     VVQEVRFYRQAQTSLAERQAQILTKTEALEKLKKENAQTPGVTLSPQQQQTMRSVLYL
                     TKQRSFSWSRLLTQLERETPRDVRIVGVKFELKEKVAADKEVTTRVPLELVIRSQKPE
                     ELTKYLYDVNQRKVFFFEPQSQGPAEKNERGGTLNETELILAGTYDPTAEHLSAPAHK
                     PPTPENAALTDRPATNSRSTSRTRGKGL"
     gene            1062..>1500
                     /locus_tag="PKZ53_23455"
     CDS             1062..>1500
                     /locus_tag="PKZ53_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43460.1"
                     /translation="MQKFWKNLFVKSRLGVAQTSYLRPQEILGILLIFVIGSGVVYFH
                     VSYLGPLDSQRVEADRKLATLGSDVDRLKTEVKRKRIDTEKFDIAMEKLKDFEVSYLS
                     DKQNGQVKLINEINELAAKNQVRLTDGMEFEPAKVELPPTEGQK"
BASE COUNT          413 a          348 c          376 g          363 t
ORIGIN      
        1 ccgcaaggag atggttgagg gtatttttcc ctcacgatgg cgagtggagg gagtttgctc
       61 cttccattcg ctgttcaata ttgattcaaa gctggagcgg cgtttcctcc cggccctgaa
      121 ggaccgggtc tccacgccga agataggatg aagaatgaag aatgaagaat tgactaacta
      181 tctgtaagta agatagatag gtgcttcgtt attcatcatt gatcatttcg ctcattctca
      241 tttcaaccaa atctgggtga tgacttcaat tggttgaggc cagttcaccc gttgtcacat
      301 acttctgaaa gacttgtgaa atcaaatagc gtatcccatt ccccactctt tgaaggcgtg
      361 gccatcaatc tttcccggaa tccatttcgg aaccgccgcc tgttttggct tggctggctg
      421 attgggattg ccgttctgct ggtggttgtc gttcaggaag tccgttttta ccgtcaggct
      481 cagacttctc tggcagagcg tcaggcgcaa atcctgacca aaactgaggc acttgaaaag
      541 ctgaaaaaag agaatgccca aaccccaggt gtgaccttgt caccccagca acaacaaacc
      601 atgcgctcag tgttatatct gacaaagcaa cgtagctttt cgtggtcccg gttgttgacc
      661 caacttgaac gtgagacgcc gcgtgatgtc cggatcgtcg gcgtcaagtt tgagttgaaa
      721 gaaaaagtcg ccgccgataa agaagtgacg accagggtgc cactggaact ggttatcagg
      781 tcgcaaaagc cggaagagtt gactaaatat ctctatgatg tcaatcaacg caaggtcttt
      841 ttctttgagc ctcaatccca ggggccggct gaaaaaaatg agcgaggtgg aacgctgaac
      901 gagaccgagt tgatcctggc cggtacctat gaccccacgg ctgagcattt gagcgcgcca
      961 gcccacaaac ctccgactcc tgaaaatgca gcgttgactg atcggccagc aaccaactcc
     1021 aggtcaactt cacgaacccg aggtaaagga ctgtaatcgc catgcagaaa ttctggaaaa
     1081 atttatttgt taaaagccga ttaggtgtgg cgcaaacttc ttatctccgg cctcaagaaa
     1141 ttcttggaat actcctgatt ttcgtaatcg gcagcggtgt tgtctatttt catgtcagtt
     1201 atttaggacc acttgacagc caaagggtcg aagctgaccg aaagctggca accctggggt
     1261 cggatgttga tcgcctcaaa actgaggtga agcgaaaaag gattgatacc gaaaagtttg
     1321 atattgcgat ggaaaagctc aaggattttg aagtgagcta tctatcagac aaacagaatg
     1381 gtcaggtcaa actgatcaac gaaatcaatg aactggcggc caaaaatcag gttcgcctga
     1441 cggatggcat ggaatttgag ccggcaaagg tggaattacc accaaccgaa ggacaaaaaa
//
LOCUS       DAOQDO010001877         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97136_length_1500_cov_2.375087, whole genome shotgun sequence.
ACCESSION   DAOQDO010001877 DAOQDO010000000
VERSION     DAOQDO010001877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97136_length_1500_cov_2.375087"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKZ53_23460"
     CDS             complement(<1..564)
                     /locus_tag="PKZ53_23460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43461.1"
                     /translation="MHSLWQDWRYSLRGLIKTPLFTLSVVFTLALSLGANTAVFSVIN
                     GVLLKPLPFPNAERLVTVRESNQAKSYNQSSFSPGKFTDLTAQVESFEHLAAYYGEDY
                     ALTDNDLPEQVSGCLVTSDFFQVLGTLPAQGRIFETRDAESGAEPVVILSHNLWKKRF
                     GANPEIIGKKISLSKQPYQVIGVMPAAA"
     gene            592..846
                     /locus_tag="PKZ53_23465"
     CDS             592..846
                     /locus_tag="PKZ53_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43462.1"
                     /translation="MKATLIQQTTVAQPICHHKAKPGKTTGESSRTGQLASIWFDENN
                     LQAGISIWKIKSLDLKWLKRARLIRDGCPQLGFRLVTRGN"
     gene            1058..>1500
                     /locus_tag="PKZ53_23470"
     CDS             1058..>1500
                     /locus_tag="PKZ53_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43463.1"
                     /translation="MIKPFEIPAAWDAVTLHRDPSWMIHLTDPHRVELRSALEFFKDT
                     THSQGLTAQWLHGNMTPQPTNFPLPTLGPLLKQVQTDLEEKYGLVLLKGTPVSDFSAK
                     ELHLLHAGIASYVGTPRPQTVFGEMVQDIKDIGQASLIERRGSKH"
BASE COUNT          387 a          396 c          357 g          360 t
ORIGIN      
        1 ggccgccgcc ggcatcaccc cgatgacctg atagggctgt ttgctgagcg agattttttt
       61 tccgatgatt tcgggattgg ccccaaatcg ctttttccac aggttgtggc tcaagatgac
      121 cactggctcg gcacctgact cagcgtcacg ggtttcaaag atccgtccct gagcaggtaa
      181 ggttccaagt acctgaaaaa aatcagatgt caccagacac ccggaaacct gttcgggtaa
      241 atcattgtca gtcaaggcat aatcttcgcc gtaataggct gccagatgtt caaagctctc
      301 gacctgtgcg gtgagatcag tgaatttccc cggtgaaaac gaagattgat tgtaactttt
      361 ggcctggttg ctttcgcgga ccgtgaccag acgctcagca tttggaaacg gcagtggctt
      421 gagcaaaacg ccattgatca cgctaaacac agccgtattt gcgccgaggc ttaatgccag
      481 cgtaaaaacc accgacaagg taaaaagcgg ggttttgatc aagccacgca aactgtaccg
      541 ccaatcttgc cacaaactgt gcatataaac tgactcccca gaaatgattt ggtgaaagcg
      601 accttgattc aacagaccac ggttgcgcaa cccatatgcc accacaaagc caaacccgga
      661 aaaaccactg gcgaatcatc ccgcacagga caattggctt ctatctggtt tgatgaaaat
      721 aacttacagg caggtatcag tatctggaag atcaaatcgc tggatctcaa gtggttgaaa
      781 cgagcacgtc tgattcgaga cgggtgcccg caactgggat ttcggcttgt cactcgtggg
      841 aactgagcgc ttgattgaac cacagaggca catagaggca tttttggctt tctccgtggc
      901 cctctgtggt ctctggggca gaattctatc tggactttgg gaaaccacct ggccagtttc
      961 tgctcataac tatttgacaa acttgggatt tcccttcttt aatcagccac caatccatat
     1021 tggatattct ggtatttttg ttcccccaat cccccctatg ataaaaccgt ttgaaattcc
     1081 ggctgcctgg gatgcggtga cccttcaccg agatccttcc tggatgatcc acctgactga
     1141 tcctcatcga gttgaactgc gttcagcact ggaatttttt aaggatacaa cccactcaca
     1201 agggctcacc gcccagtggc ttcacgggaa tatgaccccg cagccaacca attttccact
     1261 gccaaccctg ggcccgctgc tcaaacaagt ccaaactgat ctggaagaaa aatatggcct
     1321 ggtgctgttg aaaggcacgc cggtctcgga tttttcagct aaagaactgc acttgctgca
     1381 cgctgggatt gccagctatg tggggacgcc acgtccacaa actgtttttg gagagatggt
     1441 tcaagatatc aaagacatcg ggcaggcatc actgatcgaa cgccgtggca gcaaacacaa
//
LOCUS       DAOQDO010001878         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97221_length_1499_cov_3.184211, whole genome shotgun sequence.
ACCESSION   DAOQDO010001878 DAOQDO010000000
VERSION     DAOQDO010001878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97221_length_1499_cov_3.184211"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1499)
                     /locus_tag="PKZ53_23475"
     CDS             complement(<1..>1499)
                     /locus_tag="PKZ53_23475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43464.1"
                     /translation="AVKQFDDAFALLKRDVQSRTSPEFLEAASDEFRRNRRTDEEEQT
                     LKRLVTLARDEHETMKYRLQLAAFYERNNQVDTAVSVMDRLTSEFASNLGVLQEATQF
                     YWRVGLLDKSIALYQRTSTQAVGQYKRQFTLQLAKRQMDAGKLADSEKTLRTWYGENP
                     LDIEAFTLLTKVLAESNQSEALAELYKTGLKNIATGNLSSDERRNKTADLRLGLIETL
                     TKLKRHSEAVDQYIEIINRDPENTTALAAALRYSSRTGQMGRLRAYYTDLAQKADRNY
                     RWNVVLGEISIYNGEVTVASDQYRQAILNEPQRLDFRSKLAGLYEQLGRYDDAVAVLR
                     RAYEIDAGNSEWLVTLASLYIHQGKADKAVETLREAVTVRKNTLAATYFQYGQILLSA
                     GLTKESASFFEDGVEKVRRDPYRNTISSYEAGSWLTTVALTDTPLAALNKVEALANLL
                     SAEANKPNNAQPGTPRSSAESVRSTLREKLAEVVFNYATPTQKAELETA"
BASE COUNT          297 a          388 c          416 g          398 t
ORIGIN      
        1 gggcagtctc aagttccgcc ttttgggtgg gcgttgcata gttgaagaca acttcggcca
       61 gtttttcccg tagcgtcgag cgaactgatt ctgccgagct tcgcggcgtt ccaggttggg
      121 cattattggg tttattggcc tcggcgctga gcaggttggc cagggcttcg actttgttca
      181 aggccgcgag cggtgtgtct gtcaacgcca cggtggtcag ccagcttccg gcttcatagg
      241 acgaaatcgt attccgatat ggatcacggc gcactttttc cacgccgtct tcaaaaaagc
      301 tggctgattc tttggtcagt cccgccgaaa gcaaaatctg accatattga aaataggtgg
      361 ccgccagcgt gtttttgcga actgtcaccg cttcgcgcag ggtttcaacc gctttatcgg
      421 cttttccttg atgaatatag agactggcca gcgtcacgag ccattctgaa tttccagcgt
      481 caatttcgta ggcccgccga agcaccgcca ccgcatcgtc atagcgccca agttgctcat
      541 acaacccggc cagttttgag cggaaatcaa gccgctgcgg ttcattcaaa atcgcctgcc
      601 gatattgatc agaggcgact gtgacttcac cgttgtagat cgagatttca cccagcacca
      661 cattccagcg atagtttcgg tcagctttct gggccagatc ggtatagtag gcccgcaacc
      721 ggcccatttg cccggtccgt gacgaataac gcaatgctgc tgccagcgcg gttgtgtttt
      781 ccggatcccg attgatgatt tcaatgtact ggtcaaccgc ctcgctgtgg cgtttgagtt
      841 tggtgagtgt ttcaatcaaa cccagtcgca aatccgccgt tttgttgcgg cgttcgtcag
      901 agctgaggtt tccggtcgcg atgtttttga gtccggtttt atatagttcg gccagtgctt
      961 ctgactgatt ggattcagcc agcactttgg ttagcagggt aaaggcttcg atatcaagtg
     1021 ggttttcgcc ataccaggtt cggagcgtct tctcagagtc cgccagtttg ccagcatcca
     1081 tctggcgttt ggcaagctgg agcgtaaatt gacgtttgta ttgccccacg gcctgggttg
     1141 acgtgcgttg atagagggca atggatttat cgagcaaccc aacacgccag taaaactgag
     1201 tggcttcctg caacacgccc agattgctgg caaattcgct ggtgaggcga tccatcaccg
     1261 acacggcggt gtccacctga ttattgcgct cataaaaggc tgccagttgc agccgatatt
     1321 tcatggtttc gtgttcgtcg cgggccaggg taacaagccg tttgagggtt tgttcttctt
     1381 cgtcggtgcg ccgattgcga cggaattcat cgctggcggc ttcgagaaat tcagggctgg
     1441 tgcgcgactg aacatcacgt ttgagcaggg caaaagcgtc gtcaaactgt ttgacggct
//
LOCUS       DAOQDO010001879         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97242_length_1499_cov_2.577562, whole genome shotgun sequence.
ACCESSION   DAOQDO010001879 DAOQDO010000000
VERSION     DAOQDO010001879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97242_length_1499_cov_2.577562"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..>1499
                     /locus_tag="PKZ53_23480"
     CDS             194..>1499
                     /locus_tag="PKZ53_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43465.1"
                     /translation="MIDGQGVVYSFYRQSATSQQPEVDRWTFNGFAETDTQVLTIDPT
                     NPSTIYAGVRVNGLQKSTDSGQTWEKIDPGGGANFYAIEIPSKETQTIYLAKDRGLFK
                     SLDAGATWRQLTNGLGNVEAIQDVEVDPLNNNRVYAATTTGLSLSTDAGGSWQQIFQS
                     GGNEELEINPQNPGIMYFLADPGGGFSEKVLKSTDAGQSWSVVANGLEEIRFLYAMAL
                     DPNQPQNLYVGGTDGVYKSTNGGETFKPANKRLEGKIIITLAFDTLDQTSLFAGGSRE
                     GGLFYTKDGGANWADFTNGLPPPFVISILPVPQIQTIFTGVRNGLYWRKLLTEQPDTI
                     PPQVQVLAPNGQDVLRPNSRFAIAWNAIDDIQVVSQDIALSLDSGATYSMPIATDLAG
                     DASRFTWTTPREIKTQFARIKITARDAAGNVGSDTSDEDFGVY"
BASE COUNT          410 a          320 c          379 g          390 t
ORIGIN      
        1 ggttgtggtg aatggggttt tgtcgaatgt ctatgccttc gaatattgaa cctctgatca
       61 tctgatcgtt ttactgtgaa aaaaggtggt tatgccgtct gatgcaaggt gggttcaaaa
      121 aatgagaggg cctgaccatg gctctcaaag ccggtttctc cccagaataa tcctgctttg
      181 gattttgctg tgtgtgattg atggtcaagg agttgtgtat agtttttacc gtcaatcagc
      241 tacatcccaa caacctgaag tggatcggtg gacattcaac ggatttgcgg aaactgatac
      301 ccaggtactg accattgacc caacgaatcc atcaaccatc tatgctggag ttcgtgtcaa
      361 tggacttcaa aaaagcactg atagcgggca aacctgggaa aaaatcgatc cagggggcgg
      421 ggcaaatttc tacgcaattg agattccatc aaaagagact caaaccattt atttagctaa
      481 ggatagaggg ttattcaaat cactggacgc tggagcaacc tggcgacaac tcacaaacgg
      541 tttgggaaat gtggaagcga ttcaggatgt agaggttgat cctctgaaca acaaccgggt
      601 ctatgccgcg accaccaccg gactatcttt gagcactgat gctggtggtt cgtggcaaca
      661 gatctttcaa tcaggaggga atgaagaact tgagataaac cctcagaatc caggtattat
      721 gtattttttg gctgatccag gcggagggtt ttcagaaaag gttctcaaaa gtactgatgc
      781 tggccagtcc tggtcagtcg ttgcgaacgg attggaagag ataagatttc tttatgcgat
      841 ggcacttgat cccaaccagc ctcaaaatct atatgttggt gggacagatg gagtttataa
      901 aagcaccaat ggtggagaga cctttaaacc agccaacaaa cgtcttgaag gaaaaataat
      961 catcactctg gcatttgata cactggatca aacttcctta tttgctggcg gcagccgtga
     1021 gggaggattg ttttatacca aagatggtgg cgccaactgg gctgacttta caaatgggct
     1081 gcccccacca tttgtgatat caatcctgcc agttccgcaa attcaaacaa tctttactgg
     1141 ggttcgcaat ggtctttact ggcggaaact cttaactgaa cagccagaca caattccacc
     1201 tcaggttcag gtattggcgc caaacggaca ggatgttttg cgccccaata gccggtttgc
     1261 cattgcctgg aatgcaattg atgatattca ggtggtttcc caggatattg ccctttcact
     1321 cgatagtgga gcgacatact caatgccgat tgcgactgat cttgccgggg atgcctcgcg
     1381 gtttacctgg acaactcctc gtgaaataaa gactcaattt gccaggatca aaatcacagc
     1441 tcgtgatgcc gcagggaatg tggggagcga cacaagtgat gaggatttcg gagtgtatg
//
LOCUS       DAOQDO010001880         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97252_length_1499_cov_2.385042, whole genome shotgun sequence.
ACCESSION   DAOQDO010001880 DAOQDO010000000
VERSION     DAOQDO010001880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97252_length_1499_cov_2.385042"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>1499
                     /locus_tag="PKZ53_23485"
     CDS             143..>1499
                     /locus_tag="PKZ53_23485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43466.1"
                     /translation="MLSPNTLLQNRYLVVRLIGQGGMGAVYEARDQRLNHTVALKETF
                     FDEDEGRRAFEREARLLARLKHPALPRVSDYFSDKQGQFLVMEFIPGTDLGDMLKRRG
                     GAFSLEEVSRWADQLLDVIEYLHGQQPPIIHRDIKPQNLKLNQQGQLILLDFGLAKGP
                     SSATIATGGDNRSVFGYTPNYAPPEQMQGTGTDERSDLYSVAATLYHLLTGRKPVDAL
                     NRAMQLISNSSDPLTPAHQVNPAVPLQIGQALQQTMALTREQRPASVGELRQSLRARR
                     TTPLLNTSPTRKTASRQTVDSTEARSSGSEAATVIHPSQELPPLAQVIPPGKQSDTQV
                     MAPVVPVGTNFYISPSKCTVKVLSGHTSGVSGVCFTPNGQFVISGSHDTSIKIWDVET
                     NQQTGNLLPAVGPPSAIESLAVSPRGNLLLSGTRAGVLRLWDLETGRELASLKGHGSA
                     IH"
BASE COUNT          345 a          454 c          388 g          312 t
ORIGIN      
        1 tcctgactga acttactgcc gcatcccttc aaacttcccc ggttcgatgc taatctgttc
       61 ggcgctttcc ttcacacaaa aatacatcca gggttttcac agttgcgact tcacgacatc
      121 atttttcact ctttcgctca gtatgttgtc tcccaacaca ctcctgcaga atcgctatct
      181 tgtggtgcgc ctgattggtc agggcgggat gggggcggtc tacgaagctc gcgaccagcg
      241 gctcaatcat acggtggcgc tcaaagagac tttctttgat gaagatgaag gccgacgtgc
      301 cttcgaacgc gaagcccgat tgctggcccg gctcaagcat ccggcactcc cacgggtgag
      361 cgattatttt tctgataaac aaggtcagtt tctggtgatg gaatttattc cggggaccga
      421 tctgggtgat atgctcaaac gccgtggcgg tgcgttttcc cttgaagaag tctcccgctg
      481 ggctgaccag ttgctcgatg tcatcgagta cctccacgga cagcaaccgc cgatcattca
      541 ccgcgacatc aaaccccaaa acctgaaact caatcagcag ggccagctca ttctgcttga
      601 ttttggtctg gccaaaggtc cgtcttcggc cacgattgca accggaggtg acaatcggag
      661 cgtgtttggt tacacgccaa actacgcccc acccgaacag atgcagggca ccggtaccga
      721 tgaacgaagc gacctctatt cggtggcagc tacgctctat catctgttga cgggtcgaaa
      781 accagtggat gcgctcaacc gggcaatgca actcatcagc aactcaagcg atccgttgac
      841 tccggcgcat caggtcaacc cagccgttcc gcttcaaatt ggacaggcgc tccagcaaac
      901 catggcgctg acccgcgaac aacgtccagc cagcgtcggc gaactccgcc aatcgctccg
      961 ggcccgccgg acgacaccgc tcttaaacac cagtccaacc cggaaaaccg ccagccggca
     1021 aacagtggac agcaccgaag ctcgaagcag cgggagcgaa gccgccaccg tcattcatcc
     1081 gtcacaggaa cttcccccgc ttgcccaggt gattccgcct ggcaaacaga gcgataccca
     1141 ggtgatggct ccggtggtgc cggttggaac aaatttctac atcagtccct ccaaatgcac
     1201 agtcaaagtc ctgtcagggc ataccagcgg cgtcagcggc gtgtgtttta caccaaatgg
     1261 ccagtttgtg atttccggaa gtcacgacac atccatcaaa atttgggatg tcgaaacgaa
     1321 ccagcaaacc gggaatctgt tgccagccgt ggggcctccg tcagcgattg aatccctggc
     1381 ggtttcgcca cgcgggaacc tgctgctttc aggaacacgg gcaggggtac tgcggctgtg
     1441 ggatcttgaa accggacgcg agctggcatc actcaaaggt cacggaagtg ccatccata
//
LOCUS       DAOQDO010001881         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97473_length_1497_cov_2.906380, whole genome shotgun sequence.
ACCESSION   DAOQDO010001881 DAOQDO010000000
VERSION     DAOQDO010001881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97473_length_1497_cov_2.906380"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKZ53_23490"
     CDS             <1..406
                     /locus_tag="PKZ53_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ43467.1"
                     /translation="VIQALRSWIEQAPAAQTGWLGALKDQQIGRAISLIHRDPAHPWT
                     LATLAEEVAMSRSAFAARFTEIVGEPAMYYLTQWRMNLALMWLKENDMPLSDVAERLG
                     YQSEAAFSRAFKRVMGNSPGAVRRHNGNLLRE"
     gene            complement(446..964)
                     /locus_tag="PKZ53_23495"
     CDS             complement(446..964)
                     /locus_tag="PKZ53_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNJ43468.1"
                     /translation="MPKFIVGVMGAGENARSEDSTRAYQLGELIAREGWVVLSGGRAA
                     GVMEAVNRGAKSVPGSLTIGILPSRESIASEFVDVAIVTDLGNARNNVNVLSSHVVVV
                     CGKGEAGTLSEIALALKARKPVVLCLAASDETQLYEAFLKELGRELLTVCRTPEAVID
                     WVKHQRSREIAK"
     gene            1180..>1497
                     /locus_tag="PKZ53_23500"
     CDS             1180..>1497
                     /locus_tag="PKZ53_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43469.1"
                     /translation="MLRKTSISMKYACFFTFLLLSFLGFSTLISQPIEKSVQAAAPKA
                     FQTWLNRCALTVTSTNSSPVTGYQVLVTLNPGNFNYANANLDGSDIRFATSPAAPPFD
                     LNYF"
BASE COUNT          357 a          420 c          349 g          371 t
ORIGIN      
        1 ggtgatacag gcgcttcgat cctggatcga acaggcgccg gcggcccaaa ccggctggct
       61 tggggcatta aaggatcaac agattggacg agccatttca ctgattcacc gtgacccggc
      121 gcatccctgg acgctggcca cgctggccga agaagtcgcc atgtcccgct cggcgtttgc
      181 ggcacgattt acggagattg tcggggaacc agcgatgtac tacctgacac aatggagaat
      241 gaatctggcg ctgatgtggt tgaaggaaaa cgatatgccg ctgagtgatg tggcagaacg
      301 gctcgggtat cagtctgaag ctgcctttag ccgtgccttc aaacgggtta tgggaaattc
      361 tcctggcgcc gtgcggcgac acaatgggaa ccttctcagg gaataaccgc aaatagataa
      421 gtccggggag ggagtctttt tttcgctact tcgcgatttc gcgacttcgc tgatgtttca
      481 cccagtcaat cacggcttcg ggagtccggc aaacagtcaa taattcacgc cctaattcct
      541 tcagaaatgc ttcatacaat tgagtttcat cgctcgcagc caggcacaga acaaccggct
      601 tccgagcttt gagcgccagt gcaatttccg acagggtgcc ggcttcgcct ttaccacaga
      661 ccaccactac gtggcttgac agcacgttga cgttgtttcg ggcattgccc aaatcagtca
      721 caatcgccac atccacaaat tctgaagcga tactttcccg tgacggtaaa attccaatcg
      781 tcaggcttcc gggcacggat ttggcgcccc ggttgacggc ttccatcacg cctgccgccc
      841 gcccaccaga caaaacgacc cagccttcgc gggcaatgag ctcgcccaac tgataagcac
      901 gtgtactatc ttcagaacgg gcattttctc cggcgcccat cacgccgacg ataaattttg
      961 gcattggatt ttccaggaaa aaatttcggg cgacttattt tctctgctct tgttttcatc
     1021 catttagcat atactggatt tacccaggta gaatccccca tctgcccaca aacgtcacaa
     1081 tcacccaact catttaaacc tcaacccttt tgtactgcag taatcagttc gtggaaacgt
     1141 gtttttgaaa cttgatccag atatttatga agagggctta tgctgcgaaa aacttctata
     1201 tcaatgaaat atgcctgctt tttcactttt ctcctcctca gtttcctggg gttttcaacc
     1261 ctgataagcc aacccatcga aaaatcagtt caagcggcgg cgccaaaagc ttttcaaacc
     1321 tggctgaatc gttgcgcact gaccgtcacg tcaaccaaca gcagtccagt caccgggtat
     1381 caggttctgg tcacgctcaa tccgggcaat ttcaactatg ccaatgccaa tctggatggt
     1441 tcggatattc gctttgcgac gtcaccagcc gcaccaccgt ttgatcttaa ctatttt
//
LOCUS       DAOQDO010001882         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97505_length_1497_cov_2.407074, whole genome shotgun sequence.
ACCESSION   DAOQDO010001882 DAOQDO010000000
VERSION     DAOQDO010001882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97505_length_1497_cov_2.407074"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKZ53_23505"
     CDS             <1..928
                     /locus_tag="PKZ53_23505"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43470.1"
                     /translation="GCANSVTTILKRLPGVLRSEPFRAVPVRLRFQHYSRRVAILGLQ
                     PDSELRCLVDKDLNTQPIPPEGVVLTKKLAEILHVVPGDVLRVEILEGRRAMTSIPVS
                     GLVDELLGLSVYMDVRALMQLLGEDNTVSGAYLKVDPLLINQLYAELKRMPAVSGVGL
                     RDVEIESIHQTIQQNMAVSTTMLIVFAGVIAFGIVYNSARIALSERGRELASLRVLGF
                     LQAEVSFMLLGEQAILTLTGIPLGCAIGFGVCAWIPHRLNTELYRMPLVINSETYVAA
                     FLIVALAAFLSGLFVRWRIRGLDLIAVLKTRE"
     gene            1032..>1497
                     /locus_tag="PKZ53_23510"
     CDS             1032..>1497
                     /locus_tag="PKZ53_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HNJ43471.1"
                     /translation="MKNLGSKVVFGIVVLALGWAIWWGTRPTPVKVETSLVTRGPLTV
                     TVDAEGKTRVQDRFVVGAPVGGRLLRIGFRRGDQVSQQDVLAKIEPLPLSPLDPRETA
                     EATSRVLAAEALAREAVANQAHAMAEAEQARRELARAEKLAATGDVSPQEVER"
BASE COUNT          316 a          388 c          455 g          338 t
ORIGIN      
        1 gggctgcgcc aacagcgtca ccaccatcct caagcgattg cccggcgtgc tgcggtccga
       61 accgtttcgg gcagtgccag tccggctacg gtttcagcat tattcacggc gggtggcaat
      121 tttgggcctg caaccagaca gtgaactccg gtgcctggtg gataaagacc tcaataccca
      181 gccgataccg cccgaaggcg tcgttttgac caaaaagctg gctgaaattc tccacgtggt
      241 tcccggcgac gtgctcaggg tggaaatcct cgaaggccgc cgcgcaatga ccagtatccc
      301 ggtttcgggg ctggttgatg agttgcttgg actgtcagtc tatatggatg tccgggcact
      361 gatgcaatta ctgggggaag acaacaccgt ttcaggggct tatctgaaag ttgatccact
      421 cctgatcaat caactctatg cggaactcaa acgcatgccg gcggtgagtg gtgtcgggtt
      481 gcgggacgtt gaaattgaaa gtatccacca gacaattcaa caaaatatgg cggtttcaac
      541 caccatgttg attgtgtttg ccggtgtgat tgcgtttggc atcgtctata acagcgcccg
      601 gattgcgctt tctgaacgtg gtcgcgagct ggccagcctg cgggtgctcg gcttccttca
      661 ggccgaagtc agctttatgt tgcttggtga gcaggccatt ttgacgctga ctggaattcc
      721 actcgggtgc gctatcgggt ttggggtctg tgcctggatt ccacaccggc tcaataccga
      781 actctatcgg atgccgctgg tgatcaacag cgagacctat gtggctgcct ttttgatcgt
      841 ggcactggcc gcatttttgt cagggctgtt tgtcaggtgg cgaattcgag ggctggattt
      901 gattgcggtg ttgaaaacac gggagtgagg tcagggctga agaaagcggg ctgaagaaaa
      961 cgggtaattc agcgcaagcc caatgtcttc agccccaagc ccgatgtctt cagccccaaa
     1021 caggtattca tatgaaaaac cttggaagca aagttgtatt tggaattgtg gttttggctc
     1081 ttggttgggc aatctggtgg ggcacgcgcc cgacaccggt taaggttgaa accagtctgg
     1141 taacccgagg gccactcacg gttacggttg atgccgaagg aaaaacccgc gttcaggacc
     1201 gctttgtggt tggagcaccg gtgggtggcc gactgctgcg gattgggttt cgacggggcg
     1261 atcaagtttc ccagcaggat gtactggcca aaatcgaacc actcccattg tcaccacttg
     1321 acccgcgtga gacagccgaa gcaaccagcc gggtcctggc agccgaagcc ctggcccgcg
     1381 aggcagtcgc caatcaggcc catgcaatgg ctgaagccga acaggcccga cgtgagctgg
     1441 ctcgcgctga aaagctggcc gcgacgggtg atgtgtctcc acaggaagtc gaacggc
//
LOCUS       DAOQDO010001883         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97680_length_1496_cov_1.882720, whole genome shotgun sequence.
ACCESSION   DAOQDO010001883 DAOQDO010000000
VERSION     DAOQDO010001883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97680_length_1496_cov_1.882720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..837
                     /locus_tag="PKZ53_23515"
     CDS             166..837
                     /locus_tag="PKZ53_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43472.1"
                     /translation="MPKATIDMVKIDTNSWNFYSVNDHQQIWTNDAGEALSLSFFEAK
                     PDIPVAIKPEKINELRKAYRGFAKQSGAAIISVEIVSICGIPSLEVILKKPFQGRPGF
                     AFLGSVTIPLRDFSYVIRFESLEQGTTGIREATVMMLEQPIPQIDKQTSKILGWVKDP
                     YDAKFDKGALYNLADQEKYDQMFEFHPLTRVRGHLHRLKQTIQIDSYVTDYPRHSGYW
                     YKSIF"
     gene            929..1468
                     /locus_tag="PKZ53_23520"
     CDS             929..1468
                     /locus_tag="PKZ53_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43473.1"
                     /translation="MFQFVEKYLSARYSYLELSCSCGFEQKLFPNYPRYYQFENQEVM
                     PVLVCLAWCQRCQSFVESEDLATAQTILDVLQTVQTQLIPVYGLVEGDPPAPTDRCVF
                     YHAKLKLLLARQSPPKCLRYGSIDIRQFPVSTDEQPLHPGCGQFCQITRKVMSFGDSF
                     FYALYTQEGEWIPRMWAST"
BASE COUNT          402 a          342 c          334 g          418 t
ORIGIN      
        1 gttacccgat ggggactgcc cggttgtttg gtttgaccat gacgaacttt tcagcctttc
       61 agaagaacaa cttggcaatc gagaaagtgt tgaacctttg gcccatccgc ttttttcatc
      121 atttcgagaa ttgttagcca aaatctttga ataactgtgg tgtatatgcc aaaagcaact
      181 attgatatgg tgaaaattga taccaactcc tggaactttt attccgtcaa tgaccatcag
      241 caaatttgga ccaatgatgc tggagaggca ctttccctca gcttctttga agcgaaacca
      301 gatattccag tggcaatcaa gcctgagaaa atcaatgaac ttcgcaaagc gtaccgtggg
      361 tttgcaaagc aatcaggagc cgccatcatt tccgttgaga tcgtatccat ttgtggaatt
      421 cccagcctgg aagtcattct gaaaaaacca tttcaaggcc gaccaggatt tgccttttta
      481 ggttcagtga caatcccgct ccgtgatttt agctatgtga ttcgctttga atctcttgaa
      541 cagggcacaa ctggtatccg ggaagcaacc gtgatgatgc ttgaacagcc cattccccaa
      601 attgataaac aaaccagcaa gattctggga tgggtgaaag atccgtatga tgcgaaattt
      661 gataaagggg cactctacaa cctggccgac caggaaaaat atgaccagat gtttgagttt
      721 cacccactaa cccgagtcag aggtcatctt catagattaa aacagacgat tcagattgac
      781 tcttatgtta cagactatcc aagacactca ggatactggt acaagtctat attttagata
      841 gcacaactcc aatctgcttt tggtagaaca agacagattt ccaaaccagt gctggcaaat
      901 gctgagctgg gtatgtcttc tctgaactat gtttcaattc gtcgagaaat acctttcagc
      961 tcgctattcc tacctcgaac tctcatgttc gtgcgggttt gagcaaaagc tttttcccaa
     1021 ctatccacgg tactaccagt ttgaaaatca ggaagtcatg ccggtattgg tgtgcctggc
     1081 ctggtgtcag aggtgccaga gttttgttga atctgaagat ttggcaacag ctcaaacgat
     1141 tttagatgtg ctccagacgg tgcagacaca attgattcca gtttacggac tggttgaagg
     1201 cgacccgccg gctccgacag atcgctgtgt tttttatcac gccaaactca aactcttact
     1261 ggcacgtcaa tcaccaccga agtgcctccg gtatgggtca attgacatca ggcagtttcc
     1321 tgtttcaact gatgagcagc cccttcatcc gggatgcggt cagttctgtc agataaccag
     1381 gaaagtgatg tcatttggtg attcgttttt ttatgctctg tacacgcagg agggcgagtg
     1441 gattccacga atgtgggctt ctacgtgaat agccattctc attgagtcac ctgtac
//
LOCUS       DAOQDO010001884         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97747_length_1495_cov_2.972917, whole genome shotgun sequence.
ACCESSION   DAOQDO010001884 DAOQDO010000000
VERSION     DAOQDO010001884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97747_length_1495_cov_2.972917"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1112)
                     /gene="tatC"
                     /locus_tag="PKZ53_23525"
     CDS             complement(72..1112)
                     /gene="tatC"
                     /locus_tag="PKZ53_23525"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNJ43474.1"
                     /translation="MAETPVTQGTTELEPEGAMGFLDHLEELRQRIFNIAIFLVVAFL
                     VCWGFSKPIYNFLSVPVSEALRKAKFMAVKATSRVQELKELENGKVVTFIFEKGVTVE
                     KINIPTGTTVQAKIIRDPGQPIRMVTIQPIVIGKRILPEGFQLPSDLLPPDADPASQL
                     VVHTVQGAFNLYVKVAFYAAIFFSIPYILYQIYIFVSPGLYEHEKSYVWPVIIMGTFF
                     FLLGSTFGYYIAFPRACDFLIGEAENFQPFIEANEYFDLIITIIMGLGLVFEIPTVVY
                     FLARLGLVTAGFLLKIWRFAVVAIMILAAVLSPTSDIPNMMIFAVPMLGLYYFSVGIA
                     YIFNRPRKEVAS"
     gene            complement(1297..>1495)
                     /locus_tag="PKZ53_23530"
     CDS             complement(1297..>1495)
                     /locus_tag="PKZ53_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43475.1"
                     /translation="SLTKADPPVSIEPPASTIEELIPPAPEISASPVAVSRGRSAVSV
                     AALPPPPSKPKVSSMIEGFEG"
BASE COUNT          403 a          345 c          367 g          380 t
ORIGIN      
        1 attcttcatt cttcattctt cattcttcat tctcaattct tcattctcaa ttcttcattc
       61 ttcattctgt cttagctggc gacttctttg cggggacggt taaaaatgta ggcaatgcca
      121 acactaaaat agtacaaccc aagcatggga acggcaaaaa tcatcatgtt gggaatgtcg
      181 gaagttggtg aaagaacggc tgccaggatc atgatggcca ccacggcaaa gcgccagatc
      241 ttgagcaaga atccagccgt aaccagtccc aatcgagcca ggaaatacac cactgtcgga
      301 atttcaaata ccagccctaa ccccataata atcgtgataa tcaggtcaaa gtattcattt
      361 gcttcaataa aaggctggaa gttttcagct tcaccaatca aaaagtcaca ggctcttggg
      421 aaggcgatgt aatagccgaa ggttgaccca agcaggaaaa agaaggtccc cataatgatg
      481 actggccaga catagctctt ttcgtgttca tagagccctg gagagacaaa aatataaatt
      541 tgatagagga tataggggat cgaaaagaaa atcgcggcat aaaaagcaac tttgacatac
      601 aggttaaacg ctccctgtac ggtatgaacc acgagttgac ttgccgggtc agcgtctggc
      661 ggaagcaggt cagacggaag ttgaaaccct tcgggtagga tgcgtttacc gatcacaatc
      721 ggctgaatcg taaccatgcg gatcggctgg ccagggtcac ggatgatttt tgcctggacc
      781 gtggtgcccg tcggaatatt gattttctcg accgtcaccc ctttttcaaa aataaaagtc
      841 accacttttc cattttcaag ttcttttaat tcctgaaccc gactggtggc tttaaccgcc
      901 ataaatttgg ctttgcggag tgcttcagag actggaaccg agagaaaatt ataaattggt
      961 ttggaaaatc cccagcacac cagaaacgcc accacgagaa agatggcgat attgaaaatg
     1021 cgctggcgga gttcttcgag atgatcgaga aaccccatgg cgccttcggg ttccagttcg
     1081 gttgttcctt gggttacagg tgtttcagcc ataaacaaat accagttgag ggtttggggc
     1141 tgaaaggacc ccctgtttcc ccaactattt aacctttgat gaatgccatg cgttgatttg
     1201 aacgtggagt caatgccaga tactgatcaa ttgccgatgc gatagcctgg tttcttcagc
     1261 tcaggcccga gtgtctttag cccacgcctc aggagcttag ccttcaaatc cttcgatcat
     1321 cgagctgact ttgggtttac tggggggagg gggaagtgcg gccactgaaa ctgctgaccg
     1381 accccggctc acggcgactg ggctggcgga aatttctggt gctgggggga tcaattcttc
     1441 aatggtcgaa gctggcggtt caattgatac cggagggtct gctttcgtaa gcgat
//
LOCUS       DAOQDO010001885         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_97855_length_1494_cov_3.345379, whole genome shotgun sequence.
ACCESSION   DAOQDO010001885 DAOQDO010000000
VERSION     DAOQDO010001885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97855_length_1494_cov_3.345379"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKZ53_23535"
     CDS             <1..192
                     /locus_tag="PKZ53_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43476.1"
                     /translation="VVPVEVAQALKTQFNVIASPFYQGNIRLRLFCDNGKPGEGCQAA
                     TPTLEDFYFCVINNLAKGV"
     gene            195..>1494
                     /locus_tag="PKZ53_23540"
     CDS             195..>1494
                     /locus_tag="PKZ53_23540"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNJ43477.1"
                     /translation="MERPFFGPWAIICAGEILFNQRRIAPWVLMGLFSLNALLWTVSG
                     QAAAYGRPVNSDYETARLCMGFVFMTTPFFTALLMGDVILRDQWFGIDAVLLSKPIRS
                     TEYILGKFLGNFIVLSACHVCFIFVPFVVQFVPTSAVRVVPWHIIPYLKHFFWLVLIP
                     HFALAALCCTVGTLTRSVKFVYGLVTALYIAYITLQLSARNLPTRWLEAFDPLLVNWI
                     SNMAHGRSQAQLGKLVVTYNLDVLVNRGVLITFGCLCLAFLIFRFSRAGIGQVTQTGI
                     SFSLLFEQTTNTKINTVYKREVVIPPLSEHLDHRSVPSPSSASLLTAGERGKQALLQT
                     GSCQTYLIQFLAAVGCEFRLLQSERSLFVLVPVVLLLCGSHLESFGNAFGAPFYPVSS
                     VFATNSVQALVFVLSGITIFMTGEAFYRDDECNIKPVLWSL"
BASE COUNT          315 a          365 c          357 g          457 t
ORIGIN      
        1 gtggtaccag ttgaagtcgc gcaagcgcta aaaacacaat tcaacgtgat tgcctcgccg
       61 ttctatcaag gcaatataag acttcgactc ttttgcgata acggaaaacc tggtgagggc
      121 tgccaggccg caacgccaac acttgaggac ttctattttt gtgttatcaa caatttagca
      181 aaaggagtct gatcatggag cgccctttct ttggtccctg ggcgatcatc tgcgcaggtg
      241 aaatcctctt caaccaaagg cgtatcgcac cgtgggtatt gatgggattg tttagcctga
      301 atgccctcct gtggacagtc agcggtcaag ccgcagctta cggacgtccg gttaattccg
      361 attatgaaac agcccgcctg tgcatgggtt ttgtctttat gacgacacct tttttcactg
      421 cactgttgat gggtgatgtt atcctgcgtg accaatggtt tgggattgac gcggtgctgc
      481 tctcaaaacc aatccgaagt actgagtata ttcttggaaa atttttggga aattttattg
      541 tcctcagcgc ctgtcacgtt tgttttatct tcgtgccctt tgtcgttcag tttgtgccaa
      601 catctgcagt ccgggtggtg ccctggcaca ttatcccgta cttaaaacat ttcttttggc
      661 tggtacttat cccacacttt gccctggcag ccctctgttg caccgtcggt acgctgaccc
      721 gaagcgttaa atttgtctat ggcctggtca cggcattgta tattgcctat atcacgctcc
      781 aattgagtgc tcgcaatctt ccgacccgat ggctggaggc atttgaccca ttgctggtga
      841 attggatttc aaacatggct catggccgga gtcaggctca actgggtaag cttgtcgtca
      901 cctataatct ggatgtgctt gtaaatcgcg gtgttctcat aacctttggc tgtttgtgtc
      961 tggcttttct catttttcgc ttttccaggg caggcattgg tcaggttact caaacaggca
     1021 tttctttcag ccttctcttc gagcaaacca cgaacaccaa aataaacacg gtgtacaaaa
     1081 gagaggttgt catacctccg ctttccgaac acctggacca tcgctcggta ccgtcacctt
     1141 cctcagcatc tcttcttact gccggagaaa ggggcaaaca agctttactc caaactggtt
     1201 cctgtcaaac atacctgatt caatttttgg cggcagtggg gtgtgaattt cgacttcttc
     1261 aatcggagcg aagtcttttc gtgcttgttc cggtggttct gctcctgtgc ggttcacatt
     1321 tggaatcatt tggcaatgcg tttggagcgc cattttaccc ggtgtcttcg gtgtttgcga
     1381 cgaatagtgt tcaggccctg gtttttgtct tgagcggcat cacgatattt atgaccggcg
     1441 aagcctttta ccgtgatgac gaatgcaata ttaagcctgt tttatggagt ctgc
//
LOCUS       DAOQDO010001886         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98008_length_1493_cov_3.370654, whole genome shotgun sequence.
ACCESSION   DAOQDO010001886 DAOQDO010000000
VERSION     DAOQDO010001886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98008_length_1493_cov_3.370654"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1414
                     /locus_tag="PKZ53_23545"
     CDS             59..1414
                     /locus_tag="PKZ53_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ43478.1"
                     /translation="MKHILFVDDEASFRQVMAFRLKEFGYQVSTAENGARGLELFLSE
                     HPDLVLADFSMPELNGLELMQKIKAISPDTPVILITAFGDVATAVNAMKAGASDFLTK
                     PVEMDQLRLTLDRVSKVTELEDENRKLRALVEERLQFGAIIGTSKRMQDLFRILERVS
                     RTEVAVLILGESGTGKELVAKAIHQHSARKKASFVTIDCGTIPENLMESTLFGHKRGS
                     FTGAMTDQRGLFEEANSGTVFLDEVGEVPLLLQPKLLRILQEGEFTKLGETQPRRVDV
                     RVIAATNRDLSKMVAEGKFREDLFFRLNVIPVKLPALRERKEDIPLLANRFLIEAGNR
                     YGRTALRFSSEVFRIFQQYAWPGNVRELKNTVERLGILAASDIIQPQDLPEEILQVTA
                     GSGELPFSLPDSGLDLEALEKEILRQALAKHNGNRTHAARYLHMTRDTLLYRIQKFGL
                     D"
BASE COUNT          378 a          380 c          402 g          333 t
ORIGIN      
        1 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc ccaatcctat
       61 gaaacacatt ctttttgttg atgatgaagc gtcgtttcgc caggtgatgg cttttcggct
      121 caaggaattt gggtatcagg tttccaccgc cgaaaacggt gcccgtgggc tggaactttt
      181 tctttcagag caccctgacc tggtgctggc tgatttttcg atgcccgaat taaacggcct
      241 cgaactgatg caaaaaatca aagccatttc gcctgataca ccagtcatcc tgattactgc
      301 gtttggggac gtcgccacag cggtcaatgc catgaaagct ggtgcttctg attttttgac
      361 caaacccgtc gagatggatc aattgcggct cacgctggac cgggtcagta aagtgaccga
      421 actcgaagat gaaaaccgca agttgcgtgc cctggttgaa gagcggcttc agtttggcgc
      481 catcattggt acttcaaagc gaatgcagga cttatttcgc atcctggaac gtgtttcccg
      541 aactgaagtg gcggtgctca tcctgggtga aagcgggact ggcaaagaac tcgtcgccaa
      601 agcgattcac caacacagtg cccggaaaaa agcgtcattt gtcacgattg attgcggaac
      661 cattccggaa aatctgatgg aatcaacgct tttcggacac aaacgaggct catttaccgg
      721 agcgatgacc gatcagcgag ggctgtttga agaagccaat agcgggacgg tttttctgga
      781 tgaagttggc gaggtgccgt tgctcctcca gccaaaattg cttcgaatct tacaggaagg
      841 cgaatttacc aaacttggcg agacccagcc gcgtcgggtt gatgtcagag tgattgccgc
      901 caccaatcgt gacctctcca aaatggtggc tgaaggaaaa tttcgcgaag acctgttctt
      961 ccggctcaat gtgatacccg tcaagctccc tgcattacga gaacggaaag aagacatccc
     1021 gcttctggcc aaccgattcc tgattgaagc tgggaatcgc tatggtcgga ccgcgcttcg
     1081 gttttcatcc gaagtttttc ggatctttca gcagtacgcg tggccaggga atgtccggga
     1141 actcaaaaac acagtggaac ggctggggat actggccgcc agtgacatca tccagcctca
     1201 ggatctcccc gaggaaatac tccaggtcac tgccggatca ggcgaattgc ccttttcttt
     1261 gccggattcg ggattggatc ttgaagccct ggaaaaagaa atcctgcgtc aggcgctggc
     1321 caaacacaac ggaaaccgga cacacgccgc ccgctatttg cacatgacgc gggatacctt
     1381 gctctatcga attcagaaat ttgggttgga ctgagaggga agggttctgg gttccgggtt
     1441 ccgggctccg gaaaaagaca aaaaggacga aagagacgaa aaggacataa att
//
LOCUS       DAOQDO010001887         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98037_length_1493_cov_2.493741, whole genome shotgun sequence.
ACCESSION   DAOQDO010001887 DAOQDO010000000
VERSION     DAOQDO010001887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98037_length_1493_cov_2.493741"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKZ53_23550"
     CDS             complement(<1..368)
                     /locus_tag="PKZ53_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNJ43479.1"
                     /translation="MLSLTFQPLTVENWADFESLFGERGACGGCWCMWWRLKRAQFDL
                     QKGEANKTAMKEIVVASKVAPGILAYLDQTPIAWCAVAPRTDYPVLERSRVLRRVDDT
                     PVWSITCLFVAKPYRRQGVTV"
     gene            complement(599..>1493)
                     /locus_tag="PKZ53_23555"
     CDS             complement(599..>1493)
                     /locus_tag="PKZ53_23555"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNJ43480.1"
                     /translation="GYGDFVPKTWEGRLFTIIFVLVGVGGAGYVITTAAQTVLQATLN
                     ATLNRRRMYRDISQLEFHYIICGAGRVGQRLVKELQREGVDFVVIERDEHLAEKLMEQ
                     GCLVLNGDATDEDALIGARIETARAMVCCASTDADNVYITLTARGLNEKIYIVARANE
                     ESAIPKLRKAGANRVVSPVMIGTHQMSQVLLRPAVADFIELTTMTEELDLAIEQVQLA
                     SDSPLAGKKLKDSGIRSALNIIVVAVKRGQSEMIFNPSGDTIFHPDDCLVVIGDQKGL
                     SKLVQMASPGPGKTPGRFRKK"
BASE COUNT          358 a          415 c          357 g          363 t
ORIGIN      
        1 actgtcaccc cctggcggcg atagggtttg gcaacaaata aacacgtaat cgaccagacc
       61 ggggtatcgt ccacacgtcg aaggacacgt gaccgctcca gaaccgggta gtcagttcgc
      121 ggggccacgg cacaccaggc aatcggggtc tggtccagat atgccagtat gcctggtgca
      181 accttgctcg ccaccacgat ttccttcatg gctgttttgt tggcttcgcc tttttggaga
      241 tcaaattgtg cccgtttcag ccgccaccac atacaccagc agccgccaca ggcgccacgt
      301 tcgccaaata aggattcaaa gtcagcccag ttttccactg ttaagggttg aaaggtgaga
      361 gacagcatag gttggggttg gaatgaaaac cagcaagcta attacaatgt aatcgaactt
      421 ctaagaaatg ccaagctttt tttgcggggt acactggcaa aaagaacgag ggagggagcg
      481 ccgcagcgag gtagcccggc gttgagtctc agaacgtctc tgtcagcctc taaactgttt
      541 gtgttaggct ctttcagtac ttcacgactc cactcacaat acgattgcac tctcatggtt
      601 atttctttcg aaatcgccct ggggtttttc caggtccggg tgatgccatt tgaaccagtt
      661 ttgagagtcc tttttgatca ccaatcacca ccaggcaatc atctgggtgg aagatcgtat
      721 ccccagatgg gttaaagatc atttcactct ggccacgttt gacggccacc acaataatgt
      781 tgagcgcact ccgaatccct gagtctttga gtttttttcc tgccagagga gaatcactgg
      841 ccagttgcac ctgttcgatg gccagatcaa gttcctcagt catcgtggtt aattcgataa
      901 aatcagccac ggctgggcgc aataaaacct gcgacatctg gtgggtgcca atcataaccg
      961 gggataccac ccggttggcg ccggccttgc gcaactttgg aattgctgat tcctcattgg
     1021 cccgggccac aatatagatt ttttcgttca acccccgcgc cgtgagggtg atgtacacat
     1081 tgtcggcatc ggtggaagca cagcacacca tggcccgagc cgtttcaatc cgcgccccaa
     1141 tcaacgcatc ttcatctgta gcgtcaccat tgagtaccag gcacccttgc tccatgagtt
     1201 tctcggctaa atgctcgtcc cgctcaatga cgacaaaatc aactccttca cgttgaagtt
     1261 cctttaccag ccgctgtcca acacggcccg caccgcaaat aatataatgg aattctaact
     1321 ggctgatatc tcggtacata cgtcttcgat tcagagttgc attgagtgtt gcctggagca
     1381 ctgtctgagc agcagtcgta atcacatagc cggcgccgcc gaccccgacc agcacaaata
     1441 taattgtaaa taaccgtccc tcccaggttt tgggaacaaa atctccgtac ccg
//
LOCUS       DAOQDO010001888         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98055_length_1493_cov_2.267038, whole genome shotgun sequence.
ACCESSION   DAOQDO010001888 DAOQDO010000000
VERSION     DAOQDO010001888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98055_length_1493_cov_2.267038"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..>1493
                     /locus_tag="PKZ53_23560"
     CDS             288..>1493
                     /locus_tag="PKZ53_23560"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ43481.1"
                     /translation="MKCLLWNWIWLASLPGIFLLIQAEGNRIPTPPPHSAFRHPQTGQ
                     NLEILQLSPGGPVTDPPNLSVSFSQPMVTFTGLKRQSQPPTGVTLTPQPAGSWFWIGP
                     KTLVFEPETFFPMATVYQVNIGREVRSMAGNQLSTPVVWTFSTPELCITEDNADIFGY
                     SHNQHIYLEFNQRIDPAALLPFLTVVADHQTFPVRLASPEAITDDQSLTQLRNHAGSG
                     RWIVLQTKGKLPDDSTFQVHFKAGAPSAEGPLSSQHLQVRTFHTLRPMQLVESDCGGN
                     LPCDPMDWWILRFSNNIDRTTFRPEMISIAPPVEGISIDQTNHEILICCGVPEKTTYQ
                     VTISPELCDEFGQPLEQPTIVNFTVGKNDPYLFIPDSGLVTLDPAHKAELPIYTCGVE
                     SVKIEVYQVT"
BASE COUNT          354 a          386 c          345 g          408 t
ORIGIN      
        1 ttggattcag atcattattt gttttcctct ggcacttatc ctgtggtctc cgtatttctg
       61 tatcattttg attgtcgttg gcgggatagt tgcctggaaa tgggtcagct atatagccgt
      121 gaagggactg catcggttga gcgaacttca gtaatgttta gctcacactt ttgaggtttg
      181 gttcgtttcc ttattcgaac ttccatcttc gagttttgtt ataatttgcc atcttccagt
      241 gacattggcc tgttggaaaa caaatttgct cctggaaggg ccaaaccatg aaatgtctgc
      301 tttggaattg gatctggctg gcgagcctgc ctggaatttt tcttctcatc caggcggaag
      361 gcaaccgaat tcccacgcca ccaccacact cagcttttcg ccatccccaa actggtcaaa
      421 atctggagat tcttcagttg agtcctggtg gaccagttac agaccctccc aatctcagcg
      481 tttcattttc ccagccaatg gtgaccttca ctggtttgaa acgacaatca caaccaccaa
      541 ccggagtaac actcacgcct cagccggctg gttcctggtt ttggattggt ccaaaaacac
      601 tggtttttga gcctgagacc ttttttccga tggcgacagt gtatcaggtg aacattgggc
      661 gggaagtcag atcaatggcc gggaaccagc tttcgacccc agtcgtctgg actttttcaa
      721 caccagagtt gtgcataacc gaagacaatg ctgacatctt tggatattcg cacaatcagc
      781 atatctacct ggaatttaat caacgaattg atccggcggc actgctccca tttctgaccg
      841 ttgtggcaga ccaccaaacc ttcccggtgc gtctggcttc gcctgaggcc atcacagatg
      901 atcagtcact cacccagctt cgcaaccatg cgggttcagg gcgatggatc gtacttcaaa
      961 caaaggggaa gcttcctgac gactcaacct ttcaagtcca cttcaaagcc ggtgcgcctt
     1021 cggcggaagg gccactctct agccagcacc tgcaagtacg aacctttcac acgcttcgcc
     1081 caatgcaact ggttgagtca gattgcggtg ggaacctgcc gtgcgacccg atggattggt
     1141 ggatcctgcg cttctctaac aatattgacc ggacgacctt tcggcctgaa atgatttcga
     1201 ttgcaccacc agttgaagga atttcaattg atcaaacaaa ccatgaaatt ctgatctgtt
     1261 gtggggtgcc ggaaaagaca acctatcagg taaccatttc tccagagctg tgcgacgagt
     1321 ttggccaacc cttagagcaa ccgacgattg tgaatttcac ggttggaaaa aatgacccgt
     1381 acctgtttat tcctgactct ggtcttgtca cactggatcc cgcccacaag gctgaacttc
     1441 cgatttatac ctgcggggtg gaatcagtaa agattgaagt ttatcaggta act
//
LOCUS       DAOQDO010001889         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98117_length_1492_cov_3.375783, whole genome shotgun sequence.
ACCESSION   DAOQDO010001889 DAOQDO010000000
VERSION     DAOQDO010001889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98117_length_1492_cov_3.375783"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKZ53_23565"
     CDS             complement(<1..375)
                     /locus_tag="PKZ53_23565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ43482.1"
                     /translation="MQDLNIPMLISIRKIDQAQPALSEELVELLIDSVSNGASVGFLN
                     PVNPSTAREYWHNVLSTPEDELVLWVATCEETIVGSVQLALCPKENGRHRAEVQKLMV
                     HSSARGNRIASRLMQTLETYALQ"
     gene            complement(495..983)
                     /locus_tag="PKZ53_23570"
     CDS             complement(495..983)
                     /locus_tag="PKZ53_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43483.1"
                     /translation="MLVCSLFFTTAIQPVNTQQTELRTVVDYFLALPDRYAGETTREE
                     RKQLVSEDSGRLIAKDLKNGYLAISGDGGSPGFIVAIFKKPDGKYLVGVNPYFEMGED
                     FFILSFENGKWINFTRMVIPGYDPKLHYVLPQYGTTIKVQNQAEKDLYELVWKGGQFV
                     KK"
     gene            complement(1213..>1492)
                     /locus_tag="PKZ53_23575"
     CDS             complement(1213..>1492)
                     /locus_tag="PKZ53_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HNJ43484.1"
                     /translation="NEPHYAGIPVQGVTGSHAWEHHAEGDDFVQAGSLYRVMSEEEKD
                     RLIANLAGSLAQVQREDVIQRSISYFRKADADYGERLAQAVQALRSAS"
BASE COUNT          368 a          358 c          357 g          409 t
ORIGIN      
        1 ttgaagggca tatgtttcga gggtttgcat caatcgcgag gcaatccggt ttccacgtgc
       61 gcttgaatgc accatcagtt tctgaacttc agcccgatgg cggccatttt cttttggaca
      121 gagtgccagt tgaactgaac caacaattgt ctcctcacac gttgcgaccc ataacaccag
      181 ctcgtcctcc ggtgttgaca gcacattgtg ccagtattcc cttgcggtgc tgggattgac
      241 cgggttgaga aatcccaccg aagcgccatt ggaaacggaa tcaatcagta gttccaccag
      301 ttcttcactg agggcaggtt gagcctgatc aatctttcga atggaaatca acatcgggat
      361 attcaaatcc tgcataaaca attcactcgt tttctctcgt tcagttttag aagtgtttgg
      421 tttttttcgt gtgtttcgtg tatttcgtgg ttcaaaatgt ctggaagttt tcggtaaagt
      481 acttaaggta cttattactt tttcacaaat tgaccgcctt tccacaccag ttcatacaaa
      541 tctttttctg cctgattttg cactttgatg gtggttccat attgggggag cacatagtgc
      601 aatttgggat cgtaccccgg aatcaccatt cgggtgaagt ttatccattt tccattttcg
      661 aaggaaagaa tgaaaaaatc ttcgcccatt tcaaagtacg ggttcacgcc gaccagatat
      721 ttcccgtccg gctttttgaa aatggccaca ataaaacccg gtgacccacc atcgcctgaa
      781 atggcgagat atccattttt caaatctttg gctatcaatc ggccagagtc ttccgaaacc
      841 agttgtttgc gttcttcacg agtggtctcg ccagcatacc ggtcaggcaa ggccaggaaa
      901 tagtcaacta cggtgcgtaa ctctgtttgc tgggtgttga caggttgaat cgcggtggtg
      961 aaaaacagag aacagacaag catcagcaga agaacaaggc gaagggggag aacgtgtgac
     1021 ataaaaacct cggctggggc aaaggggctg gatgttttga aagcggaaga tcgttttcga
     1081 ttgtaaccca acctcttgcc tcagcaaagg ctaaacaatt gaggaaatta gatttcctca
     1141 gtctgacagt ttggtatgaa agagcatcgc tatcgcactt cagtcacaga actgggtgcc
     1201 atccctgggt acttatgaag ctgaacgtaa cgcctgaact gcctgggcga gtcgttcgcc
     1261 atagtcggca tcggctttgc ggaaatagct gatgctgcgt tggatcacat cttcacgctg
     1321 tacctgagcc agacttccag ccagattggc aatcagccgg tctttttcct cttcgctcat
     1381 cacgcggtag agactcccgg cctgaacgaa gtcatcacct tcggcatgat gttcccatgc
     1441 gtgtgaacca gttacgccct gaactggaat cccggcataa tgcggctcgt tt
//
LOCUS       DAOQDO010001890         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98135_length_1492_cov_2.910926, whole genome shotgun sequence.
ACCESSION   DAOQDO010001890 DAOQDO010000000
VERSION     DAOQDO010001890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98135_length_1492_cov_2.910926"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            510..1358
                     /locus_tag="PKZ53_23580"
     CDS             510..1358
                     /locus_tag="PKZ53_23580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ43485.1"
                     /translation="MFAESNLSQSSPVESNVSLLTGARAIPQPEAPRPVRILVVDADS
                     GTRSQVRTWLEYRTGFELTAECSTTWGAAKAIEDTHPEICVVDVGTLNGAGREFKEIN
                     PHRQQPVLIALAQTSQEALEAFELSAAAFLLKPVDRRHFDQALNRAHTQLQTAQEASL
                     LHRILDRLENIAPKSPKYLDQILVKTSELSFFVKVKEIDWIESEDHYVRLHTKKQSYL
                     IRENLSQLENQLNPRHFARIHRSMIVNLDEIQSLSPLFHGDVRLVLRTGQELTLSRRY
                     RKNVTR"
BASE COUNT          401 a          389 c          375 g          327 t
ORIGIN      
        1 aaatgccttt ggggaaattg gcaagagcat gcccgcacgt tccacaacgc gccgtacggt
       61 atcaattcgc aggtcatcaa tttcgtaaaa acgaggcggt gccaccggca tacacagggt
      121 cagcgataag atttaataag aaaaaataat ttaccttcaa cgacagaaag cctgggttaa
      181 cagtactctg gcgctgttga ttgtgggagt gcggctttct gcatctatcc acgcgcctgg
      241 agttttgtca attttggaag cctacaggaa gcacctggaa tcaacaacac cggggcactc
      301 ccgccacaaa catccctgaa agagtgagta gcgagtagtc agaaagtaaa agagtgagga
      361 aatcaggtcg tcaaaacgaa gagaaccgta agaaccggaa agttttggca ttcttgaatc
      421 aaagaaatgc gtcagcaacc acctgaatgg gtaaaagcat tgagctttcc tcccctctaa
      481 gcaccaagcc ataagttgag tcaacgtata tgtttgcaga atccaatcta agccagagtt
      541 cgcctgttga gtctaacgtt tcgcttctta ctggggcgcg ggcaattccc cagccggagg
      601 cgcctcgacc cgttcggatt ctggtggttg atgctgattc cggcacccgg tcccaggtgc
      661 ggacctggct tgagtatcga accggatttg aattgacggc tgaatgctcc accacctggg
      721 gcgcagccaa ggccatcgaa gatacccatc cggaaatatg tgtggttgat gttggaacgt
      781 taaacggcgc cggtcgggaa ttcaaagaaa tcaaccctca ccgtcagcaa ccagtcttga
      841 ttgccctggc ccaaacaagt caggaagctt tggaagcctt tgaactgagt gcggcggcct
      901 ttctcctcaa gccagttgac cggcgacatt ttgatcaggc cctcaaccgg gcacacacgc
      961 aactgcaaac ggcacaggaa gccagcttgc tccaccggat cctggaccgg cttgaaaata
     1021 tcgcccccaa atctccgaaa tacctggacc agatactggt caagaccagt gagttgagtt
     1081 tttttgtgaa agtgaaagaa attgactgga tcgaatcgga agaccactac gtcaggctgc
     1141 acaccaaaaa gcaatcgtac ctgattcgtg aaaacctcag tcaactggaa aatcagttaa
     1201 atccgcgcca ctttgcccga attcatcgct caatgattgt caacctggat gaaatccaat
     1261 cgctgtcacc actctttcac ggcgacgtcc ggctggtgct tcgtaccggc caggaattaa
     1321 cactcagccg gcgctatcgg aagaatgtga cccgatgaca gaatctcaat gctggcctgg
     1381 acttgtcacc ttgtcacctg acaaacctgg gtttgagctg ggcgttaccc acaccagatt
     1441 tccagtttga cgtcgggcgc caggagtggc cagaaccgtt ttcttgggaa gg
//
LOCUS       DAOQDO010001891         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98363_length_1490_cov_3.488502, whole genome shotgun sequence.
ACCESSION   DAOQDO010001891 DAOQDO010000000
VERSION     DAOQDO010001891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98363_length_1490_cov_3.488502"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1490)
                     /locus_tag="PKZ53_23585"
     CDS             complement(<1..>1490)
                     /locus_tag="PKZ53_23585"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43486.1"
                     /translation="LALVFTEKGDYAQAVSIYTRALAIQEKALGQSHPASILILNNLA
                     LVYRMQGEFSKAEPLYQRALAAQEKALGPVHPLVALSLHNLGGLYYEKGDYVKAEPLL
                     ERARMIREKISTGETPELATTLNALATIARDQGDYAKAEALAHRGLALYEKAFGHEHM
                     QVGFSLNFLALINASKGDLVQAEQFYNQALTIWEKLFGPEHPQVALSLFGLARIYQMQ
                     GNSQKAEPLLKRSLAIREKALGGKHPDVARTLNELATLYQTMGNYPEALRFQVKANEA
                     SERDLTENLILGSERQKILYLNQTGAYTDQTISLHTQFLPADIEALKAAVTVILRRKG
                     RVLDATSQNIEQLRSRTTPEDQLLLDELTKIRQIQSALTLKGPGTRNREDHLGELVQL
                     EKQAEDLEAKVSARSLEFQARRQPITLDQVQKAIPEDAVLVEFAGYTPFDAKLRTFGP
                     RRYVAYVVKGPKSRGTKTEPLESGSLLWADLGPADVIDQLATEFRK"
BASE COUNT          319 a          396 c          380 g          395 t
ORIGIN      
        1 tttccgaaat tcagtggcga gctgatcaat cacatcagcc ggtcccaggt ctgcccagag
       61 caggcttcca gactccaggg gttcggtctt ggttccccga cttttcggcc ctttcacgac
      121 atacgccaca taacgacgcg ggccaaaggt ccgcagttta gcgtcaaaag gagtatatcc
      181 agcaaattca accaggacag catcttccgg gatcgctttt tggacctggt caagcgtgat
      241 gggctgtcgt cgggcttgaa attccaggct tcgggcactg actttggctt caaggtcttc
      301 agcctgcttt tccaactgca ccagttcccc cagatggtct tcccggttgc gggttccggg
      361 tcctttcagg gtcagggctg attggatttg gcgaattttg gtgagttcgt caagcaacag
      421 ttgatcttcc ggcgtggttc ggctgcgcag ttgctcaatg ttttgggaag tggcatccag
      481 cacacgccct ttccgccgca aaatcactgt cactgccgct ttgagtgctt cgatatcggc
      541 tggcaaaaac tgggtgtgca gcgaaatggt ttgatcggta taggcacccg tctgattcag
      601 gtagagaatt ttttggcgtt ctgagcccaa gatcagattt tctgttaaat ctcgctcaga
      661 tgcctcgttc gctttgactt gaaaccgaag cgcttctgga taattcccca ttgtttgata
      721 cagcgtagcc agttcattca aggtacgcgc aacgtctgga tgtttgcccc ccagcgcttt
      781 ttcccggatg gccagcgacc gtttcagcaa cggctcggct ttttgggaat ttccctgcat
      841 ttgatagatc cgggccagtc caaagaggct caacgccact tgtggatgtt cgggcccaaa
      901 caacttttcc catatggtga gtgcctggtt gtaaaactgt tcggcctgta ccagatcacc
      961 tttactggca ttgattaaag caagaaaatt gagtgaaaac ccaacttgca tatgttcatg
     1021 gccaaatgct ttttcatata aagcaagtcc tcggtgcgcc agcgcttcag cttttgcata
     1081 gtcaccctga tcacgggcaa ttgtggccag cgcattgagc gtggttgcca gttccggggt
     1141 ttcaccagtt gatatttttt cccgaatcat cctggctcgc tcaagcaacg gctcagcttt
     1201 tacatagtca cccttttcat aatacagccc ccccagatta tgcagcgaaa gcgcaaccag
     1261 tgggtgaacc ggccccaatg ccttttcctg ggcagccaga gcccgttgat agagcggttc
     1321 agccttggaa aattcaccct gcatgcgata caccagcgcc agattgttga gtatcaggat
     1381 tgaagctgga tgactctggc caagggcttt ttcctggatg gccagggcgc gggtgtaaat
     1441 cgaaaccgcc tgggcataat cgcctttttc agtaaaaacc agcgccaggt
//
LOCUS       DAOQDO010001892         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98420_length_1490_cov_2.190244, whole genome shotgun sequence.
ACCESSION   DAOQDO010001892 DAOQDO010000000
VERSION     DAOQDO010001892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98420_length_1490_cov_2.190244"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PKZ53_23590"
     CDS             complement(<1..1468)
                     /locus_tag="PKZ53_23590"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43487.1"
                     /translation="MFTNSGTTWNFQQKLRASDGQFADSFGGAVAISGDTLVVGAGGA
                     DIGANNGQGAVYVFVRNGTIWSEQQKLVASDGAIFDGFGFSVSINGETIVVGSEADDV
                     GDKMSQGSAYVFVRTGTTWNEQQKLTASDGASFDFFGRSVAIYNETIVIGAPQVNIST
                     NSDQGSAYVFVRTGTTWSLQQTLTASDGAADDLFGGSVAISGETVVVGAFLDDIDSRL
                     DQGSAYVFVRTGTTWNLQQKLTDLDGMTESYFGCSLMVEGEVVVVGAFNTDLGNGVNQ
                     GAAYVFRRTGTVWEQLFKLTPLDPEESMQFGFSVAISGGLILVGAPVDTIGENINQGS
                     AYVFVLDFDTNYPSSLFVADTSNNRIQRFDGTSWLVVGSGIAGSGPGQFRLPEAVAAD
                     LSGNVIYVADTGNQRIQWSTDQGATWKVFASIGSGLNQVRSPQGLALDSSRNLYVSDT
                     GNNRVLRFSGGMPGSGVVLLPFGSAANQVNSPRGLTIDT"
BASE COUNT          401 a          438 c          294 g          357 t
ORIGIN      
        1 cagtgtcaat tgtcaatcca cgggggctat tgacctggtt agcggcgctt ccaaaaggga
       61 gtaagaccac accagaacca ggcattccac cactaaagcg taacacccgg ttattgccgg
      121 tgtcgctaac atataagttt cgagatgagt ccaatgccag cccctgtggg gatcgcactt
      181 gattgagtcc cgatccaatt gaggcaaaaa ccttccaggt agccccttga tccgttgacc
      241 actggattcg ctgattgccg gtatccgcca cataaatcac attccctgat agatctgcag
      301 caacagcttc aggtaagcgg aactgcccgg gtccagaacc agcaattcca ctgccaacca
      361 ctaaccagga agtcccgtca aatctttgaa tccgattatt actggtatca gccacaaaga
      421 gtgatgaagg gtaatttgtg tcaaaatcca aaacaaacac ataggctgat ccttgattga
      481 tgttttcccc aatcgtgtca actggtgccc caaccaggat tagtccacca ctaatcgcta
      541 ctgaaaagcc aaattgcatg ctttcttctg ggtcaagcgg ggttagcttg aatagttgtt
      601 cccagacggt ccctgttcgt ctaaatacat aggctgcccc ctgatttaca ccgttcccaa
      661 gatcagtatt gaaggcccca acgacaacaa cttcaccctc taccatgagg gaacatccga
      721 agtagctctc agtcattcca tccaggtcgg tcagtttctg ttgtagattc caggtggtgc
      781 ctgtccgcac aaacacatag gctgaaccct ggtcgagacg actatcaata tcatcaagaa
      841 acgctccaac caccactgtc tccccactga ttgcgaccga gcccccaaac aggtcatctg
      901 cggccccatc cgaggccgtc aacgtctgtt gcaaactcca ggtggtgcct gtccgcacaa
      961 acacataagc tgaaccctgg tcagaattgg tacttatgtt cacttgaggt gcccctatca
     1021 cgattgtctc attatatata gccactgagc gaccaaaaaa atcgaaactt gccccatctg
     1081 aagccgtcaa tttctgttgc tcgttccagg tggtgcctgt tcgcacaaat acgtaagcgg
     1141 agccctggct cattttatcg ccaacgtcat cagcctctga tccaaccaca atagtttcac
     1201 catttatgga aactgaaaaa ccaaacccat caaaaattgc tccatccgaa gcaaccagtt
     1261 tctgttgttc gctccagatc gttccattcc tcacaaaaac atagactgcc ccttgaccgt
     1321 tattcgcacc aatatcagcg ccacctgccc caacaacaag ggtgtctcca cttatagcca
     1381 ccgccccccc aaacgagtct gcaaattggc catctgaagc ccttaatttc tgctgaaaat
     1441 tccaggttgt cccgctgttt gtaaaaatat aaactgatcc ctcgccatca
//
LOCUS       DAOQDO010001893         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98432_length_1490_cov_2.059930, whole genome shotgun sequence.
ACCESSION   DAOQDO010001893 DAOQDO010000000
VERSION     DAOQDO010001893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98432_length_1490_cov_2.059930"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1490
                     /locus_tag="PKZ53_23595"
     CDS             <1..>1490
                     /locus_tag="PKZ53_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43488.1"
                     /translation="QALGDSNTGIPPDTHGAAGPNHLMVTLNTDVRIQDKTGRPLSTS
                     TLGAFWGRLGNLSTFDPKILYDSIQNRWIFVTMADPLEPTAAMLIGVSQTDDPTGRWN
                     LYLIQIDSNGQNWADYPSIGFNKDWIVVSCNIFPNQGGGFRGANIYVFNKADLYANGT
                     GKFTLLRGPDLTPCPAITQDANLATLFVVTNLNSAIKLSTITGAVGAEVLTQGPTISG
                     GSDTWAFATSTDIGPQKGTSVKLDTGFSWMQNVVYRNGTLWAAQTVNRPASNPTRSGL
                     QLWNLSTGGGVLQRIIVEDPTNAEMFCYPTVAVNKNSDMLIGYSRLGADEFASAHYRF
                     RGGTDALNTLRDDTLLKVGEAPYDKTFGGPTNRWGDYSSTIMDPNDLDMWTIQEYAVP
                     ATGGFDRWATWWGRVNAPQGSDTTAPTVQLTSPNGGEKLTGSNKVTISWTSSDNSLVL
                     FHDLDLSTDGGTTFATTVASGVVGTAKTFEWIVPLVESTQARVRVT"
BASE COUNT          344 a          399 c          400 g          347 t
ORIGIN      
        1 ttcaggcatt gggtgacagt aacactggga ttccacctga tactcacggc gccgctgggc
       61 caaatcacct gatggtgacc ctcaacactg atgttcggat tcaggataaa accggacgtc
      121 ccttgagcac ctcaaccctt ggcgcctttt gggggcgact gggcaatctt tcgacctttg
      181 accccaaaat tttgtacgac tcaattcaaa atcgctggat ctttgtgacg atggccgatc
      241 cgcttgagcc tacggcagcg atgttgattg gcgtttctca aaccgatgac ccaacgggcc
      301 ggtggaacct gtatctgatt caaattgata gcaacggcca gaactgggcg gattacccaa
      361 gcattggctt taacaaagac tggattgtgg tgtcgtgcaa catctttcca aatcagggag
      421 gcggtttccg cggcgccaac atttatgtgt ttaacaaagc tgacctgtat gcgaatggca
      481 ccgggaagtt taccctgttg cgcggaccag atttgacgcc ttgtcccgca attacccagg
      541 atgccaacct ggcaacactg tttgtggtga ccaacctcaa ttcggcgatc aagctttcga
      601 ccatcacggg tgccgtgggg gcagaagttc tgacccaggg gccgaccatt tccggcggct
      661 ctgatacctg ggcctttgct acctccaccg atattggtcc ccagaagggg acttcggtaa
      721 aactggatac tggattttcc tggatgcaga acgtggtcta tcgcaatggc acgctgtggg
      781 ccgcgcaaac cgtcaatcgt ccagcttcaa acccaacccg atccgggttg cagttgtgga
      841 atttatcaac cggcggaggc gtcctgcagc gaatcattgt cgaagatccg accaatgccg
      901 agatgttttg ttacccaacc gtggcggtca acaaaaacag cgatatgttg attggatatt
      961 cacgattggg tgccgatgaa tttgccagtg cgcattaccg gttccggggt ggaaccgatg
     1021 ccctcaatac cttgcgcgat gacaccctgc ttaaagttgg agaggcaccc tatgacaaaa
     1081 cttttggtgg tccaaccaat cgctggggtg attacagcag cacgattatg gatcccaatg
     1141 acctggatat gtggacgatc caggagtatg ccgttccggc caccggtggg tttgaccgct
     1201 gggcgacctg gtggggacgg gttaatgcgc cccagggatc agacaccacc gcacccacgg
     1261 ttcagttaac ctcacccaat ggcggcgaga aactcaccgg gagcaacaaa gtcaccatta
     1321 gctggacgtc atctgataac tcgctggtac tctttcacga ccttgatctt tcaaccgatg
     1381 gcggaacaac gtttgccaca acggttgctt caggcgtggt tggaaccgca aagacatttg
     1441 aatggatcgt tccgctggtt gaatcaactc aggctcgtgt gcgagtcacg
//
LOCUS       DAOQDO010001894         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98528_length_1489_cov_2.834728, whole genome shotgun sequence.
ACCESSION   DAOQDO010001894 DAOQDO010000000
VERSION     DAOQDO010001894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98528_length_1489_cov_2.834728"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKZ53_23600"
     CDS             complement(<1..564)
                     /locus_tag="PKZ53_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43489.1"
                     /translation="MHSKLLKKLHSLFHFRLLFLAIGLLWCGSLTTVTATTAILLPDD
                     DLIVSSRLIVRGQVIARESQWDASHERIYTYVTIRVDEFLKGNLSSRELVIRQLGGEV
                     GPTAQVVFLSPEFSPSEEVLLYLNPAPDGALRIAHFFMGKFSVVTDPTTGQKFVKREI
                     DQTHLESPASQTLQTGAITDSMELGAYL"
     gene            1046..1465
                     /locus_tag="PKZ53_23605"
     CDS             1046..1465
                     /locus_tag="PKZ53_23605"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43490.1"
                     /translation="MSCSRVFAQHELSEFQHVSRDQGLSQGTVMCLLQDREGYLWFGT
                     QGGLNRYDGYEFKVYYYDQQNPASLSSNFIMSLCEDPSGALWVGTNGDGLNRLDRRTG
                     KFDHFFADPKNPNSLSDNQISSLLVDHQGKLWVGTLS"
BASE COUNT          370 a          334 c          380 g          405 t
ORIGIN      
        1 aaggtaagca ccaagctcca ttgaatcggt gatcgctccg gtttggagcg tctgggaggc
       61 cggtgattca aggtgagttt gatcaatctc ccgcttgacg aatttctgcc cggtggttgg
      121 gtcagtcacg accgagaatt ttcccataaa gaaatgggca atgcggagtg cgccatctgg
      181 tgccgggttg aggtagagca gaacttcttc tgatggcgag aattccggag ataagaaaac
      241 cacctgagcc gtcggcccga cttcgccacc cagttgacga atgacaagtt cgcgggatga
      301 aaggttgccc ttgaggaact catctacccg gatcgtgaca taggtgtaaa ttcgctcgtg
      361 gctggcatcc cattgtgatt cacgggcaat gacctgccct cgaacaatca accgggaact
      421 gacaatcaaa tcgtcatccg gaagcaaaat cgcggtggtg gccgtgaccg tggtcaggga
      481 tccacaccac aagagtccaa tcgcaagaaa caacaaccga aaatggaata aggaatgcaa
      541 ttttttcaat agtttgctgt gcatatcgaa tttgaataga aactttcact ttgaattgaa
      601 tgtgaacagt ggcttgattg agttcaaaaa atgtaccacg ggtgaaagtt caattcacaa
      661 ttacttatac cagtcagtcg tcagtcgtca gtcgccagta gttcactaag cttatttgat
      721 ggaattactt gactgttccc tcatcaatgt attcattgca aaatagtatt acccggcagt
      781 acggatgatt gggttccaat gaagagaaat gcctggtttt tccagcctgc gtttatcaaa
      841 ccagtgaatt cttggtagaa tgccagcact ttttggcgaa cccaggtcct gacccagatc
      901 cccgtttgat tggtttacct caacctcagt tttttcgtgt ttgtgtttca acggcctcat
      961 ctttttggcc agttggaata caccccgttg tgtgcccatg atcagcaagt tctggattgg
     1021 atggtccatt ctcctgttga gccttgtgtc gtgcagtcgt gtgtttgcgc agcacgaact
     1081 gagtgagttt cagcatgtct cgcgggatca aggactttct cagggcacgg tcatgtgtct
     1141 gttacaggat cgggaaggat atttgtggtt tgggacccag ggtggcttga atcgctatga
     1201 tgggtatgaa ttcaaagtct attactatga ccagcaaaat ccggcgagtt tgagttcaaa
     1261 ttttatcatg tcgttatgtg aagacccgtc tggtgcgctg tgggtcggca ccaacggtga
     1321 tgggttaaac cggctggatc gtcggactgg aaagtttgac cacttctttg ccgacccgaa
     1381 aaacccaaac agtctgagcg ataaccagat ttcatcgctc ctggttgacc atcagggaaa
     1441 actctgggtt ggcacattgt cctgaatcaa ttttaagatg ctgttccac
//
LOCUS       DAOQDO010001895         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98619_length_1488_cov_4.988835, whole genome shotgun sequence.
ACCESSION   DAOQDO010001895 DAOQDO010000000
VERSION     DAOQDO010001895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98619_length_1488_cov_4.988835"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..483
                     /locus_tag="PKZ53_23610"
     CDS             193..483
                     /locus_tag="PKZ53_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43491.1"
                     /translation="MATATQSKSTSVLSTLNAFSELPREAALATFRATVASQDASAKM
                     VRSLIDASLANQEAANRFAKDYLTSMYSAQMEWMKKSTELAEKVLTASPSLD"
     gene            701..1132
                     /locus_tag="PKZ53_23615"
     CDS             701..1132
                     /locus_tag="PKZ53_23615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43492.1"
                     /translation="MPRKTSPKESTPAAKAPSKKLTMPDPAYLVKEVSEQIWKQTEDF
                     WGQMTRNPAFLAAISTATEQSHALTGRVQELVSQSMKSMNMVTRDDIQAVIRRLDALS
                     EQLADLNDKLDELTPQPAPAPKPVAKPKATTPARRTKKASD"
     gene            1125..>1488
                     /locus_tag="PKZ53_23620"
     CDS             1125..>1488
                     /locus_tag="PKZ53_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43493.1"
                     /translation="MTNGFLNYARESFETWQWHADAVYQQWMKNPLLINIVRENQNQM
                     LQTYALSRHWSEQLFGTSSLLESSLHTWNQATRAMIPIAATPSQVVYQEDRLRVLRFQ
                     PRKPKEFGIPLLIVNSLVN"
BASE COUNT          429 a          396 c          330 g          333 t
ORIGIN      
        1 attttctggc attatttcaa gcaaaccaca gccacactct ctgtttttca cataattttg
       61 aaaggttaac tagttgaaaa taaacaattt aaaattttta tctcgcaatt gcgaaaaaaa
      121 attattgaca gataaattcc cgatgtctat tctccgggcc atcacaactt acatttcgga
      181 ggatttgaca ccatggctac tgcaactcaa tcaaaatcaa catcggttct ttcaactttg
      241 aatgcgtttt ctgaacttcc ccgtgaagcc gcgctggcga catttcgggc tacggtcgcg
      301 tctcaggatg cctctgccaa aatggtgcgg agcctgattg atgccagcct ggccaaccag
      361 gaagccgcca atcgttttgc caaggattac ctgaccagca tgtattccgc tcagatggaa
      421 tggatgaaaa agtcaacgga gcttgcggaa aaggtgctca ccgcatcacc gtctctggac
      481 tgagcaccca actcacagcc aaggcaacag gcgagatgaa gaattccagg aatgaagaat
      541 gaagaatgaa gaatgaagaa tgaagaatga agaatgaaga atgaagaaac tgattttagc
      601 tgctctattt ctcaattctc aattctcaat tcttcattcc tggaattctt cattcggtcg
      661 gttggcttcg gctttttcag aggaggaaag ggaatctcat atgccacgca aaacatcacc
      721 caaagaatca acaccggcag ccaaagcgcc cagcaaaaaa ctgaccatgc ccgacccggc
      781 ctacctggta aaggaagtca gtgaacaaat ctggaaacag accgaggatt tttggggaca
      841 aatgacccgc aatccagcgt ttctcgccgc gatttccacg gcaaccgagc aatcgcacgc
      901 actgaccgga cgggttcagg aactggtctc ccagtccatg aaatcaatga acatggtcac
      961 ccgcgatgat attcaggcgg tgattcgccg gctggatgcc ctttccgaac aacttgccga
     1021 tctcaacgac aaactcgacg aactcacgcc gcaacctgcc cctgcaccaa agccagttgc
     1081 caaaccaaag gcaaccactc cagctcgacg gacgaaaaaa gccagtgact aacggttttt
     1141 taaactacgc gcgcgaatca tttgaaacct ggcaatggca cgcggacgcg gtgtatcagc
     1201 agtggatgaa aaatccactg ctgatcaaca ttgtgcgtga aaaccagaac cagatgctgc
     1261 aaacctacgc tctgagccgc cactggtctg agcagttgtt tggcaccagt tcgcttctgg
     1321 aatcaagcct gcacacctgg aatcaggcca cccgggcaat gatccctatc gcggccaccc
     1381 cctctcaggt tgtataccag gaagatcggt tgcgagtact gcgttttcaa cctcgcaagc
     1441 ccaaagaatt tggaatcccg ctcctgattg tcaattcgct ggtgaacc
//
LOCUS       DAOQDO010001896         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98774_length_1487_cov_2.924581, whole genome shotgun sequence.
ACCESSION   DAOQDO010001896 DAOQDO010000000
VERSION     DAOQDO010001896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98774_length_1487_cov_2.924581"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKZ53_23625"
     CDS             <1..264
                     /locus_tag="PKZ53_23625"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNJ43494.1"
                     /translation="VFGILLIANHLLLPAHQGQTLGKWLVGIRIIQTSFAPVSVFHIL
                     KRHLVGYPLSMIFGLGFLWMLWDRRQQGWHDKLAHTLVVRLDV"
     gene            563..>1487
                     /locus_tag="PKZ53_23630"
     CDS             563..>1487
                     /locus_tag="PKZ53_23630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ43495.1"
                     /translation="MPLLFVQQANGMNYEVAAQKVRTTIGRSSRNDICLNDPFASRVH
                     AEVRRENESFFITDMGSANGTYHNGVRMSGTLPLQIGDIIRIGETQIHFRDSDSTGSY
                     SANILLSDADWTTAPEATIMGRSGTNKKDSLLTSVISEMAGRDKLSDKTGILTPQLGV
                     LDKQRDLLAVVSKVVETLLSERSLDETLKLILDLVFDAVAAERGYIFLTSPTGELLLK
                     AARTRTGNTDQLVTDEVKISRSIIDTVLSECTSVLTSDAQQDPRFSQRNSIMLSNIRS
                     IMAVPLAHTGDGVLGMIYVDSHYGTSRFSQED"
BASE COUNT          358 a          361 c          357 g          411 t
ORIGIN      
        1 gtatttggta ttcttttgat tgccaatcat ctgctgcttc cagctcatca gggacaaacg
       61 cttggaaaat ggctggttgg cattcgcatt attcaaacca gctttgctcc ggtctcagtg
      121 ttccatatcc tcaaacgcca tctcgttgga tatccactct cgatgatttt tggtcttggt
      181 tttttgtgga tgttgtggga ccgccggcaa caaggatggc atgataaact tgcacataca
      241 ctggtcgttc ggttagatgt ctagttacct gcacaaaatt atgaattatg aatcagccta
      301 tcctggcttc catcagatct gaatctcata attcagaatt cataactggt ttacctcctc
      361 gcgttcaccg ttcttttgat tccacacttc cctgtggatc aatcaccagc cattgtccat
      421 aacctacttc tgacagaatc gagtgctact ggctgaatgc aaagctgtgt tgtcttgagc
      481 ctgttcaggt cgtcttgttg aaaaaagtca atcatagctt cccccaactc ttctggttgt
      541 cacgttaaag gccgatttgg atatgccact tttatttgtc cagcaagcca atggaatgaa
      601 ttatgaggtt gccgcccaga aagtgcgcac cacgattggg cgctcttcgc gcaatgatat
      661 ttgcttgaat gatccatttg cttcgcgcgt tcatgccgaa gtccgccggg aaaacgagag
      721 cttttttatt acggatatgg gcagtgccaa cggcacctac cataacgggg ttcggatgtc
      781 aggcaccttg ccgcttcaaa ttggggacat cattcgaatt ggcgaaacac aaatccactt
      841 tcgtgactca gattcaaccg ggtcatacag tgccaacatt ttgctttcag acgctgattg
      901 gacaaccgca cccgaagcga cgattatggg tcggagcggc accaacaaaa aagacagcct
      961 cctgacctcg gtgatcagtg aaatggctgg ccgggataag ctcagtgata aaactgggat
     1021 tctgacgccc cagctcgggg tgctcgacaa gcaacgcgat ttgctggctg tggtgagcaa
     1081 ggttgttgaa acgctgcttt cagagcgttc attggatgaa accctcaaat tgattttgga
     1141 tctggttttt gatgccgtag cggctgagcg agggtatatc tttctcacca gtccaaccgg
     1201 tgagttgttg ctcaaagctg cccgaacgag gacgggaaat accgatcaac tggtcactga
     1261 cgaagtcaaa atcagccggt caattattga tacagtctta agcgaatgca cctcggtttt
     1321 gacctctgat gcccaacagg atccgcgatt ttcacagcgg aactcgatta tgctcagtaa
     1381 catccggtca attatggcgg ttccgctcgc gcatactggt gatggagtgt tgggcatgat
     1441 ctatgttgac tcgcattacg gaaccagccg gttcagccag gaagatc
//
LOCUS       DAOQDO010001897         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98800_length_1487_cov_2.407821, whole genome shotgun sequence.
ACCESSION   DAOQDO010001897 DAOQDO010000000
VERSION     DAOQDO010001897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98800_length_1487_cov_2.407821"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1166
                     /locus_tag="PKZ53_23635"
     CDS             387..1166
                     /locus_tag="PKZ53_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43496.1"
                     /translation="MQPNYPMQHSYGQQVGPHFERPHNGQWTVALIWFGIGLAGLMAA
                     FAPAVIEMDMMNGGYALIMLGLFVTMFGLIGGIMYANRARTLDRIFSGDRVLVWWRYA
                     PDEWRKFAVTEFQIAKSEKVPLYILTVVISLIIGGIFFIADPKAGGIVFLGLMGLMAL
                     IGLLVLVLPRLRYAKNQETIGETIISMDGVYINGDFHSWNFFSASVDSVRVLDADPLL
                     LEFTYSFVTRTGRQEENVRVPIPRGQEAVAGRVVEFFTHQA"
BASE COUNT          360 a          334 c          380 g          413 t
ORIGIN      
        1 cgcggcaatc gccagacgtg acaagcgtaa aataggggtg ttcgtgatag tgccagtcaa
       61 tgaatttgta gttatattcg gtttcggtcg caacgacgcc gtcaaacacc cttttttgga
      121 aggtatcccc ttgaaattga gccacattgg agtattccat ttccgcaagc tccttcaatt
      181 tgcacaagat tggtttgacc gaatcctaag taccttaccg aaaactcaaa gacattttga
      241 accacgaaac acacgaaata cacgaaaaaa accaaatact taccaaattc aatctgtcag
      301 taggtttatg tcaaagtcct taaaggtggc attcagcttg ctcattaaac tcaatggcga
      361 attttcaccc aaggagggct cagtgtatgc aaccgaatta cccgatgcaa cacagttatg
      421 gacagcaggt cggaccgcac ttcgaacgtc cgcacaatgg acagtggacc gtggcgctga
      481 tctggttcgg cattggtttg gctgggctga tggcggcgtt tgctcctgcc gtgattgaaa
      541 tggatatgat gaacggcggc tatgccctga ttatgctcgg tctttttgtg acgatgtttg
      601 gtttgatcgg aggcattatg tatgccaatc gagcccgcac cctggatcga attttctccg
      661 gagaccgggt gctggtctgg tggcgctatg ctcctgatga atggcggaaa tttgccgtga
      721 ccgaatttca aatcgccaaa tctgaaaaag tacctctcta cattttgaca gtggtgattt
      781 cattgatcat cggcggtatc tttttcattg ccgatccaaa ggctggtggc attgtttttc
      841 tgggtttgat gggtttgatg gcattgattg ggttgcttgt gctggttctg ccccggctcc
      901 ggtatgccaa aaaccaggaa accattggcg aaaccatcat ttcaatggac ggtgtttata
      961 tcaatggtga ctttcactcc tggaattttt tcagtgccag cgttgactca gtcagagtcc
     1021 tggatgcgga cccgctgttg ctggagttta cctactcgtt tgtcacccga actggacgcc
     1081 aggaagaaaa tgtccgtgtc ccgatcccac gcgggcagga ggcagtggct ggtcgggtgg
     1141 tggagttttt cacacaccag gcgtaggact tggggctgag ggccgaagaa gttaatcggt
     1201 tccaaatcgt tacagtccac aaaagtgtgc taaaccaggc cgtgagcatt cttttccagg
     1261 tgcatgatgc tgaaatcaaa gcttcacaat tttgacctgg agggtttgtc atgcaacgtt
     1321 cagctatttt tgtgtgtgcg gtgtttttct gtttgtttac actggtttca cttccaatca
     1381 aagctcagga actatctgat tttgaagaag atgaaagtga aattcttgcc agtgacctga
     1441 ctccggtgca gatcgaaaag atcaaacgag ccgaagccgc cgccgat
//
LOCUS       DAOQDO010001898         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_98802_length_1487_cov_2.379190, whole genome shotgun sequence.
ACCESSION   DAOQDO010001898 DAOQDO010000000
VERSION     DAOQDO010001898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98802_length_1487_cov_2.379190"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..756
                     /locus_tag="PKZ53_23640"
     CDS             304..756
                     /locus_tag="PKZ53_23640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43497.1"
                     /translation="MKTRLKILLLLGFLYLLPGTQSTQSVSGNPTRTIVSYCWVSHHP
                     QSFDGKIISVSGILSMYPNAMALSDPACVLEPDNPTCRLILADDFQPDLETASWLQQA
                     TDFQTDRQQKMAEVIVQGKFSYDKYDCFQPKFTLVISEIKPLSEAFDQ"
     gene            791..1273
                     /locus_tag="PKZ53_23645"
     CDS             791..1273
                     /locus_tag="PKZ53_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43498.1"
                     /translation="MIQFQEVPPNEGVKDNYRPLASINQTVAAIAQQHQLTIDEFDED
                     GLGWSHMVVLRASDGKQIRVIQHLQSHWSETSTMIEMVPDDPTTPIVLDEFLAWLGIG
                     TADLTWLSPVITLPTYRLWRQDDNGQTFHIETVSGLGEAIRRVKAFEDLGHKQCYWYE
                     "
BASE COUNT          384 a          337 c          352 g          414 t
ORIGIN      
        1 ccattcgagg aaaaaaaacc acatagcagt caatattgcg ctatgggtac tgactggttg
       61 tttcctcggg ctgtttttgt ggggagttgg atttcttgtt ccgagtcttc ctggtcagat
      121 aggacaaacc gttcgcctgg gagttgttat gactgtatgt agcctttgct ttggattcat
      181 cggactggtg acggaactga tccggtttcg gaacaaacca cagctagcaa ttcaaagggg
      241 aacatttcaa aattcacccc gaacaaagaa caagaaacgc agccggagga ggtcaccacc
      301 cgtatgaaaa ctcgactgaa gattttgctg ctgcttggtt tcttgtattt attgccaggt
      361 actcaatcca cacaatccgt ttctggcaac cccacccgga caatcgtcag ttattgctgg
      421 gtaagccacc atccgcagtc gtttgacggg aaaatcattt ctgtaagcgg aattctgtca
      481 atgtatccca atgcaatggc tctttctgac cctgcctgcg ttcttgaacc ggataacccc
      541 acgtgccggc taatcctggc tgatgatttt caaccggatc tggaaactgc ttcctggctg
      601 caacaagcaa cggattttca aactgatcgc cagcagaaaa tggcggaagt gattgttcag
      661 gggaaattta gctacgacaa gtatgactgt tttcaaccga aattcaccct ggttatttct
      721 gaaatcaagc cgctttctga ggcatttgat cagtgatgcc agtcagtagt cagttggtca
      781 ttcactttat atgattcaat ttcaagaagt tccacccaat gaaggtgtga aagacaatta
      841 ccgtccgctg gcttcgatca atcagacggt agctgccatt gcacagcagc atcagttgac
      901 cattgatgaa tttgacgaag atggtcttgg ctggtcacat atggttgttt tgcgggcaag
      961 tgacggcaaa caaattaggg taattcagca tttgcaaagc cactggtcag aaacctcaac
     1021 gatgattgaa atggtacctg atgatccaac aaccccaatt gtgcttgatg aatttctggc
     1081 ctggcttgga atcggaactg ctgatctgac ctggctttcc ccggtgatta cacttccgac
     1141 ctatcgcctc tggcggcagg atgacaacgg acaaaccttt cacattgaga ctgtttcagg
     1201 attgggtgaa gccattcggc gggttaaagc ctttgaagat cttggccata aacagtgtta
     1261 ctggtatgaa tgagagcaaa agggtcagtc gcatgtagtc aatccaggaa ggggagggaa
     1321 ttttgatgca ccaggttttg gttcttgtga ttttagtttt gccgatattg gacctgatcg
     1381 ttcaacctgt tcctgcattt cgccaaactg tgaaaagcac tattctcaga ggaaccatta
     1441 cctttgaaag ccaactgggc aatcgtcctg cttctaactc ctttatt
//
LOCUS       DAOQDO010001899         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99168_length_1484_cov_2.207838, whole genome shotgun sequence.
ACCESSION   DAOQDO010001899 DAOQDO010000000
VERSION     DAOQDO010001899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99168_length_1484_cov_2.207838"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1484)
                     /locus_tag="PKZ53_23650"
     CDS             complement(<1..>1484)
                     /locus_tag="PKZ53_23650"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ43499.1"
                     /translation="QEAETGLQFARKTRFGGTVRVFIGLLTLIRSLRGLPADFRSFTD
                     EAEFEHDLEHDPRLALPNYWYWFRKMQARFFAKDYASAVAAALKAHKLRATTPTFFEN
                     SEFHFFGALVRAAGYDQTTGDEQASHLEAVVAHHKQLAVWAENCPETFECRLKLAAAE
                     LARIEGRELDAMQLYEAALTGARAQGFVQIEGLASELAAAFYSARGLETSASAHVRNA
                     RYCYLRWGAEGKVGQLDQAHPHLREDLTVRSSPTIASPVEQLDLATLFKVSQAVSDEI
                     ILEKLIEKLMVISVEHAGAERGILILQRNDKLSLEAEATTADGKVQVTTGKMEVTSAV
                     LPESILRYVTRTLDSVLLDDATAAHSFSDDPYLNRRRTRSVLCLPLLKHTCLSGVLYL
                     ENNVASHVFTPARISMLNLLASRAAISLENAYLYCDLQQAKVYLAEAQRLSLTGSFGW
                     KVHTGEIFWSEETFRIFGYDPTLKPEVSLVLARIHPEDINLVQQ"
BASE COUNT          324 a          402 c          435 g          323 t
ORIGIN      
        1 ctgctggaca aggttgatgt cttccggatg aatccgcgcc agaaccagac tgacttctgg
       61 tttgagtgtc gggtcatacc caaaaatgcg aaaggtttcc tcggaccaga aaatttctcc
      121 ggtgtgtact ttccagccaa aactgccggt caggctgagc cgctgggctt cggccagata
      181 gaccttcgcc tgttgtaaat cacaataaag ataggcattt tccagggaaa tcgccgcccg
      241 cgaggccagc aggttcaaca tcgaaatccg ggcgggtgta aacacatgcg aggcaacatt
      301 gttttcaaga tacagcaccc cgctgaggca ggtatgtttc agcagaggca aacagagtac
      361 tgaacgtgtg cgccggcggt tcaggtacgg gtcgtcagaa aatgagtggg ctgcggtggc
      421 atcatcgagc agcaccgaat cgagcgttcg ggtgacatag cgcagaattg actcaggaag
      481 taccgctgag gtgacttcca ttttgccagt ggtgacctgg accttgccat cggcggttgt
      541 ggcttcagct tcaagcgaga gtttgtcatt tcgctgcagg atcaggatgc cacgctccgc
      601 gccggcatgc tcgaccgaaa tcaccatgag tttttcgatc aatttctcaa ggatgatttc
      661 atctgaaact gcctgggata ctttgaacaa cgtggccaga tccagttgct cgacgggtga
      721 ggcaatcgta ggcgaagacc gtaccgtcag gtcctcccgc aggtggggat gtgcctgatc
      781 aagctgcccc actttcccct cggcccccca tcggagataa caataccggg cgttgcgcac
      841 atgggcactg gcgctggtct ccaagccccg tgcgctataa aaggcagcgg ccagttcgct
      901 ggccagccct tcgatctgga caaacccctg ggcacgtgct ccggtcagcg cggcttcata
      961 gagctgcatg gcgtcgagtt cgcggccttc aatgcgggcg agttcagccg ctgccagctt
     1021 cagccggcat tcaaatgttt ccgggcagtt ttcggcccac accgcaagtt gtttgtggtg
     1081 cgcgacaact gcctcaaggt gactggcctg ttcgtcacca gtggtctggt catatccggc
     1141 ggctctgacc agggcgccaa agaaatgaaa ttcgctgttc tcaaagaagg tgggtgttgt
     1201 cgcccgaagt ttgtgtgcct tgagcgctgc cgccacggct gaggcatagt ccttggcaaa
     1261 aaaccgcgcc tgcattttgc gaaaccagta ccagtagttc ggcagtgcca gccgtggatc
     1321 atgttccaga tcgtgctcaa attcagcttc atcggtgaaa gatcggaaat cggcgggcag
     1381 accacgcagg gaacggatga gggtgaggag cccgataaag actcgaaccg tgccgccaaa
     1441 gcgggtcttt cgagcaaatt ggaggccggt ttcagcttcc tggt
//
LOCUS       DAOQDO010001900         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99251_length_1483_cov_3.546919, whole genome shotgun sequence.
ACCESSION   DAOQDO010001900 DAOQDO010000000
VERSION     DAOQDO010001900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99251_length_1483_cov_3.546919"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..569
                     /locus_tag="PKZ53_23655"
     CDS             273..569
                     /locus_tag="PKZ53_23655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43500.1"
                     /translation="MTFRISQTIDHTAQNADVLVEGILTQAEAKLLENICTDLMDRVQ
                     SNITINVSGVTIQDDGAKELLHRIKDRCTSLTRCSFFPRNLIEDFHNGEFQPCY"
     gene            544..1317
                     /locus_tag="PKZ53_23660"
     CDS             544..1317
                     /locus_tag="PKZ53_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43501.1"
                     /translation="MANSNPVTNRLTLALGALLLIVVTVPPALAQKKPAQQAGSQTTL
                     AEKAKQSHQDLIEATESYKASLEKLIALYETDAQNQAERLGKLKTLFEQGLIARRELD
                     TQQQAIDSLQAKISNAQNQITQADSMIVEVLALDEQLNRIAQSTARLSKYTTVSIKYT
                     GTRGWSISEASFLQQFYLTRFGRQLPITAFGQSATHDKLGYDHHNAMDVGVHPDSAEG
                     QALMEHLRANGIPFQAFRQAVPGSSTGAHIHIGFPSRKF"
BASE COUNT          408 a          409 c          311 g          355 t
ORIGIN      
        1 ttcttcattc ttcattcttc attcttcatt cttcattctc aattcttcat tcttgacgac
       61 gctttggtat atttttgaca gagtgtgaat cttttgacgc accctggaat agagcaggcc
      121 gggaattcga ctgaaacttc tgagcggtca gaccgtaatt tgtgcgtccg caagcacttg
      181 ctgtgaaagc cggaaaaata ttgcacgctt tggcaaggta tttgcacaac ccaaagccgc
      241 aacattcatc acagcatcag gaggaacggc aaatgacatt tcgaatttca caaaccattg
      301 accacaccgc tcaaaacgca gacgttttgg tcgaaggcat tctgacccag gctgaagcga
      361 aactgctgga aaacatttgc accgacctga tggaccgtgt gcaaagcaac atcacgatta
      421 atgtttcagg ggttaccatc caggatgatg gggcaaaaga attactgcac cggatcaaag
      481 acaggtgtac ctcactgacc cgctgctcgt tttttccccg aaatctgatt gaggattttc
      541 acaatggcga attccaaccc tgttactaac cggttgactc tggcgctcgg tgccctgttg
      601 ctgattgtgg tgacagttcc ccccgcactg gcgcaaaaaa aaccagctca acaagctggt
      661 tcccaaacga cactggctga aaaagccaaa cagagccatc aggatttgat tgaggccact
      721 gaaagctata aagccagtct tgaaaaattg attgccctgt atgagaccga tgctcaaaac
      781 caggcagaac gccttgggaa actcaaaacc ctttttgagc aaggtctcat cgctcgccgc
      841 gaacttgaca cccagcaaca agccattgat tctctccagg ccaaaatcag taatgcccaa
      901 aatcagatca ctcaggccga ttcgatgatt gtcgaagtcc tcgccctcga cgaacaactc
      961 aaccgaatcg ctcaatccac cgcccgcctt tcgaaataca cgaccgtttc catcaaatac
     1021 accggcacgc gcggctggtc catttcagaa gccagtttcc tccagcaatt ttacctcacc
     1081 cgatttggcc gtcagctccc gattacggcc tttggtcagt cagccaccca cgacaagtta
     1141 ggctatgacc accataatgc catggacgtc ggtgttcatc cggatagcgc cgaaggtcag
     1201 gcgctgatgg aacacctgcg cgccaacggc atcccgtttc aggcatttcg tcaggcggtt
     1261 ccaggatcct caaccggtgc tcacatccac attggattcc cttcccgaaa attctgaaaa
     1321 ctcatccaac cgaagaatta acaaccttaa caaattcttt gcatttcctt aaccaaccag
     1381 gatagaagtt cctttatggt gttggctaca aaaaatcaga gatggtgtcg gctaccgaga
     1441 atcaaattta tctgtaaagg agtgtcctat gcacaagaca ctc
//
LOCUS       DAOQDO010001901         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99259_length_1483_cov_3.228291, whole genome shotgun sequence.
ACCESSION   DAOQDO010001901 DAOQDO010000000
VERSION     DAOQDO010001901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99259_length_1483_cov_3.228291"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1442
                     /locus_tag="PKZ53_23665"
     CDS             93..1442
                     /locus_tag="PKZ53_23665"
                     /inference="COORDINATES: protein motif:HMM:NF016090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43502.1"
                     /translation="MERVRAAVAHYNQLLEAHPSVLTDSRDFIKDRIQAEKMIFGGRP
                     LSPYLRPHFVSAAQWRHITDVCERIWSCVEVVGQAVTTEPRLNADLGITPGEAELIAI
                     DPGFRGISVTSRLDSFLTESAYSFVELNAENPAGISYCEVMGKIFMELPVMQEFIKTF
                     PVTPLYARHLLLETLLRAFKDWSGGRDKPNIAIVDWEGLPTQCEFEQFREYFIHSGYA
                     CEIVAPEKLDYTNGRLRTGDFEIDLVYRRVLTNDILENPEKCKALIDAYRAHDVCVVN
                     GFRTKFVHKKMFFGVLTDDANAHLFTEEQRAAIKAHIPWTRRCQEGHTMYAGQSIDLV
                     SYTRANRDTLVLKPNDEYGGKGIFIGWESTESEWDDAIQTALAGDYLVQERVSTAQEI
                     FPHYTPEGDITFIEQLVDLDPLLFYGKVGGAFTRLSSNALCNVTSGGGMVPTFILND"
BASE COUNT          335 a          389 c          377 g          382 t
ORIGIN      
        1 gcagctcact cgttgtcacc catctgcctt cttccgtccc cgcacctctc aaaatgggat
       61 tctttctcct tactttctga aagacaaaca ctatggaacg agttcgtgct gccgttgctc
      121 actataatca actccttgag gcgcacccat cggttttgac cgattcccgg gattttatca
      181 aagaccgcat tcaggctgag aaaatgattt tcggaggccg accgttatcc ccctatctgc
      241 gcccccactt tgtttctgcg gcccagtggc gacacattac cgacgtgtgc gagcggatct
      301 ggtcgtgcgt cgaagtggtt ggccaggcgg tcaccaccga accacgcctg aacgccgacc
      361 tgggcatcac gccaggtgaa gccgaattga ttgctattga tccaggcttt cgtggtattt
      421 ccgtcacttc ccgacttgat tcttttctca ccgaaagtgc ctatagcttt gttgaactca
      481 atgctgaaaa cccggcaggg atttcctatt gcgaggtgat gggcaaaatc tttatggaat
      541 tgccggtgat gcaagagttt atcaagacat ttccagtaac cccactctat gcccggcatt
      601 tgctgcttga gaccctgctg cgggctttta aggactggag tggcggtcgc gacaagccaa
      661 atattgcgat tgtggattgg gaagggttgc ccacccaatg cgaatttgaa caattccgcg
      721 agtactttat ccactccggg tatgcctgtg aaattgtggc gcctgagaag ctggactaca
      781 caaatggtcg gctgcgcact ggtgattttg aaattgacct ggtctaccga cgtgtcctga
      841 ccaatgacat ccttgaaaac ccagagaaat gcaaagcgct cattgacgcc tatcgtgccc
      901 acgatgtgtg tgtcgtcaat ggatttcgga ccaagtttgt ccacaaaaag atgttttttg
      961 gggtcctgac cgatgacgcc aacgcccatt tgttcaccga agagcagcgg gcagcaatca
     1021 aggcccacat tccctggact cgccggtgtc aggaaggcca cacaatgtat gctggtcaat
     1081 ccattgatct ggtcagttat actcgcgcca accgcgatac cctggtgctc aaacccaatg
     1141 atgagtatgg cgggaaaggg atctttatcg gttgggaatc aaccgagtca gagtgggacg
     1201 atgccattca aaccgccctc gctggtgatt atctggtgca ggaacgtgtc agtacagcgc
     1261 aggaaatctt cccccattac acgcccgaag gtgacatcac gtttattgag caactggttg
     1321 accttgaccc attgctgttt tatggaaaag ttggtggcgc tttcacccgt ctgtccagta
     1381 acgcactgtg caacgtcaca tctggtggcg gcatggttcc aacattcatc ttgaatgact
     1441 aattttcaag ggtttgggac tcagggttcg gagttggggt cag
//
LOCUS       DAOQDO010001902         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99394_length_1482_cov_2.959355, whole genome shotgun sequence.
ACCESSION   DAOQDO010001902 DAOQDO010000000
VERSION     DAOQDO010001902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99394_length_1482_cov_2.959355"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..>1482)
                     /gene="uvrA"
                     /locus_tag="PKZ53_23670"
     CDS             complement(58..>1482)
                     /gene="uvrA"
                     /locus_tag="PKZ53_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ43503.1"
                     /translation="RLRFLKDVGLEYLTLDRLTSSLSGGEAQRIQLATHLGSSLVGAL
                     YVLDEPSIGLHSRDGHRLIQVLQRLRDIGNTVLVVEHDPDMMLAADYLIDIGPGAGEF
                     GGEVIFQGTLPELLKHPTSLTAKYLRKENSIKKPQTRRTPGNRKITISGAREHNLKNL
                     TVEIPLGLMVCITGVSGSGKSTLVHDVLYRGLQREPENGKRVPTFEIDGKQYIDEVIC
                     VDQSPIGRTPRSNPATYLKVYDGIRELFAATREAQNAGWTASHFSFNLPGGRCETCQG
                     DGTVTVEMQFLADVELICDDCHGQRFRPEVLAVKYRGKSIHDVLNLTVREAISFFSNV
                     KKISDRLKILDEVGLGYLRLGQSATTLSGGEAQRIKLATFLSRPGTSRMLYIFDEPTT
                     GLHFDDINKLLFAFRRLLDLGASLVIIEHNLDVIKVSDWVIDLGPEGGHAGGTVIGCG
                     TPEDIAQVSHSHTGRFLAKVLTTA"
BASE COUNT          346 a          404 c          359 g          373 t
ORIGIN      
        1 tttcattctt cattcttcat tcttcattct caattcttca ttcttcattc ttcatggtca
       61 ggctgtggtt aaaactttag cgagaaaccg gcctgtatgt gaatgtgata cctgggcaat
      121 gtcttcaggg gtgccacacc caataacagt tcctccggca tggccacctt ctggtccgag
      181 gtcaatgacc cagtctgaaa ccttgatcac atccagatta tgctcaatga tgacgagcga
      241 ggcgcccagg tcaagcaacc gccgaaatgc aaaaagcagt ttattgatat catcaaaatg
      301 aagcccggtg gtgggttcat caaagatata gagcatgcgg cttgtccctg gtcgcgacaa
      361 aaacgtggcg agtttgatgc gttgggcttc accgccagac agtgtggttg ccgactgtcc
      421 aagccgtaaa tacccaaggc caacttcatc gaggattttg agacgatcac tgattttttt
      481 gacgtttgag aagaaactga ttgcttcccg aaccgtcaga ttgagcacat catgaatgct
      541 tttcccacga tacttgaccg ccagcacttc agggcggaac cgctggccat gacagtcgtc
      601 acaaatcagt tccacatccg ccaaaaattg catttcgacg gtcactgtcc catccccctg
      661 gcacgtctcg caccggccac ccggcaggtt gaatgaaaaa tggctggcgg tccatcccgc
      721 gttttgggct tcgcgcgtgg cggcaaagag ttcgcggatt ccatcataca ctttgaggta
      781 ggtggccggg tttgaacgtg gggttcgacc aattggggac tggtcaacac aaatgacttc
      841 gtcaatatat tgcttgccat caatttcaaa cgtcgggacg cgttttccat tttcaggttc
      901 gcgttgcagc cctcgataca gcacatcatg caccagggtt gactttcctg aaccagaaac
      961 ccccgtgata cacaccatca gtcccagggg aatctcaacc gtcaggtttt tcaagttgtg
     1021 ttcacgggcg ccagaaatcg ttatttttcg gtttccaggt gtccgccggg tttggggttt
     1081 tttaattgag ttttctttcc gcagatattt ggccgtaagt gatgtcgggt gcttgagcag
     1141 ttccggcaac gttccctgga aaatgacttc accgccaaac tcacccgctc cgggtccaat
     1201 gtcaatcaag taatctgccg ccagcatcat gtctggatca tgctcgacga ccaaaacggt
     1261 gttcccaata tcgcgcaatc gctgcagtac ctgaatcagg cggtgaccat cgcgtgaatg
     1321 cagcccaatg cttggttcat ccagcacata gagcgcgccc accagcgaag accccagatg
     1381 ggttgccagt tggatccgct gggcttcacc cccggaaagg cttgacgtca gccggtcaag
     1441 cgtcaggtat tccaacccga catctttcaa gaaccgtaac cg
//
LOCUS       DAOQDO010001903         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99610_length_1481_cov_2.345722, whole genome shotgun sequence.
ACCESSION   DAOQDO010001903 DAOQDO010000000
VERSION     DAOQDO010001903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99610_length_1481_cov_2.345722"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PKZ53_23675"
     CDS             <1..1188
                     /locus_tag="PKZ53_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43504.1"
                     /translation="VFRQGKVFDHDLDGTLTVRLRHGVEKQGNHLLMKKRETATHNDD
                     PSEIDDRLRPLLTADGEQYQVLLQEVIEKHAEPLIQKIIGAKLHVSSTEAYDHSEAEV
                     LEDFCQEAILKLVAYLNQSRQKPQEYCIKSLDDFVATTAFNVYNKYLREKYPLRYKLK
                     RQLYYLLNNRKALALWKEGHEFISGLSKWRERRPAERHSQALWSLREDDSKFVTSRLK
                     GRAPSELPPDSLLIHVFQWLGHSLELNLLVDVVAELLKIKDQPITKTPEEDENKRVAQ
                     NHAHHPGPLTRIEHQQILKQLWEEIEILRVNQRWALLCSLRDEQGNGLITFFPSCGIA
                     SPRQIAQTLKIEIEELMMLWDKFPLKDSEIAARLGVSTQQVINLRKSARERLERRIRK
                     ANQ"
     gene            1287..>1481
                     /locus_tag="PKZ53_23680"
     CDS             1287..>1481
                     /locus_tag="PKZ53_23680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43505.1"
                     /translation="MAEHLSNETIQLSLHRELAAESRFNADIHFADCSSCRERLGEGD
                     KLQALYQAFQADLTISTEETC"
BASE COUNT          428 a          368 c          359 g          326 t
ORIGIN      
        1 gtttttcggc aaggaaaagt gttcgaccac gatttagatg gaactctgac ggtgcggctc
       61 aggcacggag tggaaaaaca aggcaatcat ctgctgatga aaaagcgaga gacggccacc
      121 cataacgacg atccctcgga aattgatgac aggcttcggc cactactgac cgctgatgga
      181 gagcaatatc aggttctcct gcaagaggtg attgaaaaac acgctgaacc gttgattcaa
      241 aaaatcattg gtgccaagct ccatgtgtct tcgaccgaag cttacgacca ttccgaagcg
      301 gaagtcctgg aggacttctg ccaggaagcg attctcaagc tggtggcgta tctcaatcag
      361 tcacgccaaa agcctcagga atactgcatc aagtcccttg atgattttgt cgcaaccacg
      421 gccttcaatg tttacaacaa gtatctgcgc gaaaaatacc cgctccgcta taagctgaag
      481 cggcagcttt actatttgct caacaaccgt aaggcgcttg cgctttggaa agaaggacac
      541 gagttcatca gcgggctttc caaatggcga gagcggcgtc cggccgaacg tcacagccag
      601 gctttgtgga gtttgcgcga agacgactcg aagttcgtga catcccggtt aaaaggccga
      661 gcgccttcgg agcttccacc ggattccctg ttgatccacg tgtttcaatg gctggggcat
      721 tcgctggaac ttaatttgtt ggttgatgtc gttgcggaat tactgaaaat caaggatcaa
      781 cccatcacca aaacccctga agaagacgaa aacaaacgag tcgctcaaaa ccacgcccac
      841 caccccgggc cgttgacgcg aatagaacat cagcagattt tgaaacagct ctgggaggag
      901 atcgaaatat tgcgtgtaaa ccagcgttgg gcgctgttgt gcagtttaag agatgagcaa
      961 ggcaatggat tgataacctt ttttccgtcc tgcggaattg cttctcccag gcaaattgcc
     1021 caaaccctga aaatcgaaat tgaagaactg atgatgcttt gggacaagtt cccactcaag
     1081 gattcggaaa tcgctgcgcg attgggcgtc agcacacagc aagtcataaa cctgcgaaaa
     1141 tccgcacgag aacgactaga acgtcgaata aggaaagcaa atcaataaag cccgaactca
     1201 ttgaataaaa aggcgatacc ggctttttta cagagatgag taataattcc agccgatggt
     1261 catcttaatc tgctgagaaa atagatatgg ccgaacattt atccaacgaa accattcaac
     1321 tttccctgca ccgggaacta gctgcggaat ccaggtttaa tgctgacatt cacttcgccg
     1381 attgctcatc ctgccgtgaa cggcttggag aaggtgacaa gcttcaagct ctgtaccaag
     1441 cctttcaagc agacctcaca atctcgaccg aagaaacctg c
//
LOCUS       DAOQDO010001904         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99614_length_1481_cov_2.202665, whole genome shotgun sequence.
ACCESSION   DAOQDO010001904 DAOQDO010000000
VERSION     DAOQDO010001904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99614_length_1481_cov_2.202665"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKZ53_23685"
     CDS             <1..358
                     /locus_tag="PKZ53_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43506.1"
                     /translation="SGFRVPGSGFRVPGSGFRASGFSNFRPFCPFRPFCPFSFSLLNP
                     EPGFQCRNIGTRRNIVGTGVGTPSAEGITIGILIFLNCCNSIAYKIFSSSALVWQLNC
                     HCMGIEWRHDNDENNG"
     gene            339..611
                     /locus_tag="PKZ53_23690"
     CDS             339..611
                     /locus_tag="PKZ53_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43507.1"
                     /translation="MMKITADSTDRSIIVKLEGTLTTDWVKELLLYWHETFVYEKNRS
                     RLIDLRGITFIDDAGKALLALLYRDGAELIADDLLIRSIVDEITRL"
     gene            655..>1481
                     /locus_tag="PKZ53_23695"
     CDS             655..>1481
                     /locus_tag="PKZ53_23695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ43508.1"
                     /translation="MTAQVVHAQTVAQSDTPQPVVRERIGVSSTKPYALTMQEAITLA
                     LENNRDIEVERIGVQRKEFDLAAAKGVYDPTMDLQFSYNRRDTPVASFLAGGENGKVE
                     SSDLSNTTTFTKRLPWQGGTFKATFENNRATTDNQFYALSTEFQTGLRFEFTQPLLRN
                     RSTDSARRQIKISSKQLALSDIQFRQRVIEIISQVNSAYWDLVFSYRNEEIKRESVEL
                     AKTQLEQIQRLVDKGQMAPSEMISANVEIERRTDEAEAALEAVQRAENSLKSLILQP"
BASE COUNT          401 a          361 c          343 g          376 t
ORIGIN      
        1 ttccgggttc cgggttccgg gttccgggtt ccgggttccg ggttccgggt tccgcgcttc
       61 cgggttttcg aactttcgtc ctttttgtcc ctttcgtcct ttttgtcctt tttcttttag
      121 tcttttaaac ccggaacccg gttttcaatg ccgaaatatc ggcacgcgcc gaaatatcgt
      181 cggcactggc gtcggcacac cttcggcgga aggcataaca atcggcatct taatcttctt
      241 aaattgctgt aattcaatag cttacaaaat attttcatcg tcggcactgg tttggcaatt
      301 gaattgccat tgtatgggca tcgaatggag acacgacaat gatgaaaata acggctgaca
      361 gcacagacag atcaatcata gtaaaactgg aaggcacact caccaccgat tgggtgaaag
      421 aactcctgct ttactggcac gaaacctttg tttacgaaaa gaaccgctcc cggctcattg
      481 atctcagagg cattactttt attgacgatg ccggtaaagc gctcctggcg ttactttacc
      541 gagatggagc cgagttgatt gccgatgatc tgctgatcag aagcattgtt gacgaaatta
      601 ccagacttta ggaagggtga aactatgtta cggaatgtca tacttatttt tcttttgact
      661 gctcaagtag ttcacgcaca gacagttgca caatcagaca ctccacagcc agttgttcgg
      721 gaacgcattg gtgtcagcag caccaaaccc tatgcgctta ccatgcagga agctatcacg
      781 ctggcactgg aaaacaatcg cgatatcgaa gtcgaacgca ttggcgtaca acgcaaagag
      841 tttgacctgg ctgccgccaa aggtgtgtat gatccaacta tggacctgca attttcatac
      901 aatcgccgtg ataccccggt ggcttcgttt ttggcaggag gtgaaaatgg gaaagtggaa
      961 agttcagatc tgagcaatac aaccactttt accaagcgcc ttccctggca gggcggaacg
     1021 ttcaaagcaa cgttcgaaaa caatcgggcc accaccgaca accagtttta tgccctcagc
     1081 actgaatttc aaactggatt gcgatttgaa tttacccagc cgctgttgcg caatcggagc
     1141 accgattcag cccgacgtca gatcaaaatt agttcaaaac aactcgcact ctcggatatt
     1201 caatttcggc aacgagttat cgaaattatt tcacaggtca acagtgccta ttgggacctg
     1261 gtgtttagct atcgcaatga agaaatcaaa cgtgaatcag ttgagctggc taaaacccag
     1321 cttgaacaaa tccagcggct ggttgacaaa ggacaaatgg cacccagcga aatgatttcg
     1381 gccaatgttg aaatcgagcg ccgaaccgat gaagccgaag ccgcccttga agctgttcaa
     1441 cgagcggaaa acagtctgaa atcattgatt ttacaaccag a
//
LOCUS       DAOQDO010001905         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99616_length_1481_cov_2.144460, whole genome shotgun sequence.
ACCESSION   DAOQDO010001905 DAOQDO010000000
VERSION     DAOQDO010001905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99616_length_1481_cov_2.144460"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKZ53_23700"
     CDS             complement(<1..649)
                     /locus_tag="PKZ53_23700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ43509.1"
                     /translation="MTLTTPISVLIVDDDCLARDCMRSVVEASSGFSVIGECTTGLGV
                     IKAMEDLSPSLILLDIQMPMMNGLELVESLGELLPLVVFVTAFDRYAVTAFDHHAVDY
                     VLKPIDRLRLEKSLQRAKVILETRQKHAFNQKLIACLHDLKLKYAASSEQILIRSAGN
                     SLFLKVQSIDWVEAEGSYVRLHIGKNSHLIRENIGSFLSRMDPKIFIRIHRSVAVN"
     gene            complement(646..>1481)
                     /locus_tag="PKZ53_23705"
     CDS             complement(646..>1481)
                     /locus_tag="PKZ53_23705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ43510.1"
                     /translation="IVAMNWQDIQAGEFTLHNYSVELLMRINLSLLIYLVLIFTTHAF
                     DYRQESQDRLIRELQLQTQLDQTRLNALKIQLQPHFLFNVLNSISVLQHQDLPGAQKM
                     VHRLGEFLQKILYQSNDLQVRLEQELEWLECYLDIERIRHRERLQVTVIAPAEALEVY
                     VPNLILQPVVENAVRYAMAGPHPTGEIVIRALLAENRLQVEVIDNGPGLAVTDLNSSS
                     ARKGIGLANTRARLEQLYGRDARLELLPAAGGGVNVTLTLPIQRESEPQFNHFPESVM
                     I"
BASE COUNT          403 a          368 c          346 g          364 t
ORIGIN      
        1 cgttgaccgc caccgaccga tgaatccgga taaaaatttt tggatccatc cgggatagaa
       61 atgatccgat gttttcgcgg atcaaatgcg agttttttcc aatatgtaac ctgacataac
      121 tgccttcggc ttcaacccag tcaatgctct ggactttgag gaagaggctg tttccggctg
      181 atcgaatcag gatttgttcg ctactcgcgg catatttcaa ttttaagtcg tgcaaacagg
      241 caattaattt ctggttgaag gcgtgctttt gccgggtttc aagaatgact tttgcccgct
      301 gaagtgactt ttccagacgc aggcggtcaa ttggttttaa gacataatca accgcgtgat
      361 ggtcaaaggc agtcacagca tagcgatcaa aagcagtaac aaaaaccacc agcggcaaga
      421 gttcgccaag agattcaacc agctccaatc cattcatcat tggcatctga atatccagga
      481 ggatcaggct gggtgacaga tcttccatgg ctttgatgac gccaagacca gtcgtgcatt
      541 caccaatcac tgaaaatcca gaactggctt cgacaactga ccgcatacaa tcacgagcca
      601 gacaatcatc atcaacaatc aacacagaaa taggagttgt gagggtcata tcatcacact
      661 ttcaggaaaa tgattgaatt ggggttcaga ttcccgttga atcggcaagg taagcgttac
      721 gttcacgcca ccgccagcgg caggtaatag ttcgagccgc gcgtcccgac catacaattg
      781 ttccagccgg gccctggtgt tggccagacc aatacccttt cgggcagagg aggaattgag
      841 atcagttaca gccagacctg gcccattatc aatgacttca acctggagcc ggttttcagc
      901 cagcaatgct cgaatgacaa tttcacccgt cggatgcggc ccagccatgg cataccggac
      961 cgcattttca accactggtt gaagaattaa atttggcaca tagacctcca gggcttctgc
     1021 aggcgctatc acggtcacct gcaggcgctc acggtggcga atgcgctcaa tatccagata
     1081 acattccaac cactccagtt cctgttccaa tctcacctgc aggtcatttg actggtacag
     1141 gattttctgt agaaattcac ccagccgatg caccattttt tgggcgccag gcaagtcttg
     1201 atgctgtaac acagagatgg agttcaagac attaaacaaa aagtgtggct gcaactgaat
     1261 tttcaaggca ttgagcctgg tttgatcaag ctgggtttgg agttgtaact cccggatcaa
     1321 tcggtcctgc gattcctgcc gatagtcaaa cgcgtgcgtg gtaaagatca gcaccagata
     1381 gatcagtaaa ctcaggttga tccgcatcag caattcaact gagtaattat gtaaggtaaa
     1441 ttcgccagcc tggatgtctt gccagttcat ggcaacaatt c
//
LOCUS       DAOQDO010001906         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99697_length_1480_cov_2.880702, whole genome shotgun sequence.
ACCESSION   DAOQDO010001906 DAOQDO010000000
VERSION     DAOQDO010001906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99697_length_1480_cov_2.880702"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PKZ53_23710"
     CDS             <1..1422
                     /locus_tag="PKZ53_23710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43511.1"
                     /translation="EAGQTIWFNRQWMEFTGKSIESQLGTAWQQVIHPEDQEPTLGIC
                     HQAALKHQKFRMEWRMKRFDGQYRIMLDVGVPRFLETGKYVGHIGTCIDITDVKQAEA
                     ERGQVARLESLGTLAGGIAHDFNNMLTSMLGNISLARRAKSPVQLETRLTEAERAIGR
                     AQYLTKQLLTFAKGGEPIKSQVNLQALVVETVTFCLRGSSVEPIFRFAPHLPLVDADP
                     GQLGQVIQNLVINARDAMPAGGNLVVSASVITSPQDRSELLRLTFDDEGDGIPANILN
                     RIFDPYYTTKPTGHGLGLAVVYSVVRQHGGTINVTSSQNKGTQFEILLPISPLVEQTP
                     EPLSVPVTPTFENCRILVLDDDDGVREITLEMLYMLGIQATGASRGEQLVELYTQALH
                     SRTPFDLLLLDLTIPGGKGGIWTLNELKKINPQIRAIVCSGYSQDPALAYPADFGFIG
                     RLVKPFSLEELQAQIRQALTKDG"
BASE COUNT          363 a          387 c          385 g          345 t
ORIGIN      
        1 gaagccggtc aaaccatctg gtttaatcgt caatggatgg aatttacagg taagtcgatt
       61 gaatcccagt taggtacagc ctggcaacag gttatccatc ctgaagatca agaaccaacc
      121 ttgggaatct gtcatcaagc tgctctcaag catcagaaat ttagaatgga atggcgaatg
      181 aaacgatttg acgggcaata tcgcatcatg ctggacgttg gggtacctcg gtttttagaa
      241 accggcaagt atgtcgggca catcggcacc tgcattgaca ttacggatgt caagcaggct
      301 gaagccgaac gtggtcaggt ggcccggttg gaaagcctgg gaacactggc gggaggcatt
      361 gcccatgatt tcaacaacat gctgaccagt atgctcggca atatttcact ggctcgtcgg
      421 gcgaaatctc ccgtacagct cgaaacccgt ctcaccgaag ctgaacgagc gattggccgg
      481 gcgcaatatc tgaccaagca gttgctgact tttgccaaag gcggtgaacc cattaaaagt
      541 caggttaatc tgcaggcgct ggtggttgag acagtgacgt tctgccttcg tggatcatca
      601 gttgaaccga tttttcggtt tgccccccat cttccgctgg ttgatgcaga tccaggtcag
      661 cttgggcagg tgattcaaaa tctggtgatc aatgcccgcg acgccatgcc agccggggga
      721 aatctggtgg tctcggcatc ggtcatcacc tctccccagg accggtcaga actgttgcga
      781 ctgacctttg atgacgaagg agacggcatt ccagccaata ttttgaatcg aatctttgat
      841 ccatattaca ccacgaaacc aaccggccac ggactgggac tggcagtggt gtattcggtc
      901 gtccggcaac acggcgggac catcaatgtg acttccagtc agaacaaagg cacccagttt
      961 gagatcttgc ttccgatttc gcccctggtt gaacaaacgc cagagccgct ctcggtccca
     1021 gttaccccca catttgaaaa ttgccggatt ctggttttag atgatgatga cggagtccgt
     1081 gaaataaccc tcgaaatgct ctacatgctt ggcatccagg caactggtgc cagtcggggc
     1141 gaacaactgg ttgaactcta tactcaggcg ctccacagcc gcaccccatt tgacctcctg
     1201 ttgctggatt taacgattcc aggtggtaaa ggcgggattt ggacactcaa cgaactcaag
     1261 aaaatcaatc cacagattcg ggccattgtg tgtagcggct attctcagga cccagctctg
     1321 gcgtatccag ccgactttgg atttatcgga cgactggtca aacccttttc gcttgaagaa
     1381 cttcaagcac aaatccggca ggcactcacc aaagacggtt gacggtgatt agattattcg
     1441 tcgcatggca acccggcggg tccgccatcc catccgggct
//
LOCUS       DAOQDO010001907         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99850_length_1479_cov_2.361657, whole genome shotgun sequence.
ACCESSION   DAOQDO010001907 DAOQDO010000000
VERSION     DAOQDO010001907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99850_length_1479_cov_2.361657"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1197)
                     /locus_tag="PKZ53_23715"
     CDS             complement(223..1197)
                     /locus_tag="PKZ53_23715"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43512.1"
                     /translation="MTDQPVEFSLPQVMMTLSAVAYTYENSPLADIRTHLIEQLATPG
                     YATQGNWSLFWGPGVTDQTMMYCARDTANPNRFVLSIRGTDLMDIKNWVQDLWVNLVP
                     FHKGGEPFDGNPQISHGTLLGLNHLISMMTQTNPGDFVTAESGPNLLQYFANRADTAE
                     TEIIVTGHSMGACLGVVLAVWLQDVLAANGRQVTVSSCTFASPTPGDPVFATYAENRL
                     GSRNQRYFNQLDIIPKAYNDILGMKTTPTKLEFGDDIWVIPILDEVDRLVRDSHFTHP
                     GDHFPIQQELRTEGGWFTQAGFEHDINTYLSILGAPKVENLMPPKKHK"
     gene            complement(1194..>1479)
                     /locus_tag="PKZ53_23720"
     CDS             complement(1194..>1479)
                     /locus_tag="PKZ53_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNJ43513.1"
                     /translation="VVFAANFSAMFDSGKVDAPDELRTNRPSSNYIHFGYAQHRCLGE
                     YISQVQLRETIKRLLKLKNLQKAAGSDTQPVAPGNLPTEMTITFDPGDAQ"
BASE COUNT          366 a          364 c          371 g          378 t
ORIGIN      
        1 tttgtctaaa gaattcaagt ttcagaataa aaccatccgg tttgcccgat atggggtgat
       61 tgcctggatc tttgactatg tcaataaaat gggcactcaa gatttaagaa atcaacctaa
      121 tcctccccga aaatcgatca agcggttgcc tcgacaaaac ttataaaggt ggctgattga
      181 ataatccacc accttcaata agccagttta tttctaaaac cgtcacttgt gttttttcgg
      241 cggcatcagg ttttcgacct tcggggcacc taaaattgag agataggtgt tgatgtcgtg
      301 ttcaaacccc gcctgggtaa accagccgcc ctcagttcgg agttcctgct ggatcgggaa
      361 gtgatctccg ggatgcgtga aatggctgtc tcgcaccagc cgatcaactt catccaaaat
      421 cggaatgacc caaatatcat ctccgaattc aagtttggtc ggggttgttt tcattcccag
      481 aatgtcattg tatgcctttg gaatgatatc gagctggtta aaataccgtt gattgcggct
      541 tcccagacgg ttttcggcat aggtggcaaa aaccgggtcg ccgggcgttg gtgaagcaaa
      601 ggtacaggat gaaacagtta cctgccttcc gttcgcggcc agaacatcct gcaaccacac
      661 ggccaaaacc acgcccaggc aggcgcccat actgtgcccg gtcacgatga tttcggtctc
      721 ggcagtgtca gcccggttgg caaaatactg aaggaggttg ggaccagatt ctgctgtgac
      781 aaaatcacct gggttggtct gggtcatcat cgagatcagg tgattaagcc caagcagcgt
      841 cccatgcgaa atttgtggat ttccatcaaa gggttcgccg cctttatgaa acggcaccaa
      901 attgacccac agatcctgca cccagttttt gatgtccatc aaatctgtgc cacgaatcga
      961 aaggacaaac cggttgggat tggctgtgtc acgggcacaa tacatcatgg tctgatctgt
     1021 cacgcctggt ccccagaaca atgaccagtt tccctgagtc gcatagccag gagtggcgag
     1081 ttgctcgatt aagtgcgttc gaatgtcagc cagcgggcta ttttcatagg tgtaagccac
     1141 ggctgacagc gtcatcatca cctgtggcaa actaaattca acgggttgat cggtcattgt
     1201 gcatctcctg ggtcaaacgt gatcgtcatt tcggtcggga gatttccagg tgccaccggc
     1261 tgggtatctg aacccgctgc tttttgcagg ttcttcagtt tgagcaggcg tttgatcgtt
     1321 tcgcgcaatt gaacctgaga aatgtattct cccaggcacc gatgctgggc atatccaaag
     1381 tgaatgtagt tggatgaagg ccgattggtc cgtaattcat ctggcgcgtc aaccttccca
     1441 ctatcaaaca tggccgagaa gttcgcggca aaaacgact
//
LOCUS       DAOQDO010001908         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_99967_length_1478_cov_2.625439, whole genome shotgun sequence.
ACCESSION   DAOQDO010001908 DAOQDO010000000
VERSION     DAOQDO010001908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99967_length_1478_cov_2.625439"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1478)
                     /locus_tag="PKZ53_23725"
     CDS             complement(<1..>1478)
                     /locus_tag="PKZ53_23725"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNJ43514.1"
                     /translation="ATAWRKWAGTTDFLNPLKQRLQTLGWPVSGVEKLVATALADPTW
                     QTLGALDAATRMVTSLVQAGGIQRGKQARRVLESFFEQATGESQDRTLEIPNAYWFVR
                     AAPGNQQLYMRGAVLVRVKGCRASRQMAGKAAQDQAEGRPALSPELVAALNESPTRPG
                     RELLRLLAQDGILTPVVVGFALMCSAAGTVIEALLFRGLFDIGGQLTLPEQRLVALAA
                     LLGFLALSMFLDLPIMTAMLRLGRHLEIRLRVAFLEKIPRLGDRYFQSRLTSDMAERS
                     HSIQGIRVLPHIGGQFIQAMSQLVMTTAGIIWLDPSSTVLAMTVAVVAVLVPMLILPQ
                     LAEKDLRVRVHTGALTRFYLDSLLGLIPIRTHGAERAIRREHESLLVEWVRAQIGLLR
                     ATVVIDGFLSTVGFLLVVLLVFHSLNRGQEPGGMLLLLYWALSLPTLGQEVALIARQY
                     PVIRNMTLRLLEPIGALEEPTDDDDIDRSQMSTLPLVQTLQA"
BASE COUNT          341 a          430 c          417 g          290 t
ORIGIN      
        1 aagcttggag cgtctgaacc agtggcagcg tgctcatctg actgcggtca atgtcgtcgt
       61 catcagtcgg ttcttccaaa gcgccgatag gttccagtag ccgaagcgtc atgttgcgaa
      121 tcaccggata ttggcgggca atcaacgcta cctcctgacc cagtgtcggt aagctcaacg
      181 cccagtagag caaaagtagc attccgcccg gttcctggcc tcgattcaga gagtgaaata
      241 ccaggagcac caccagcaaa aaaccaaccg ttgagagaaa cccatcaatt acgaccgtgg
      301 cgcgcagaag gccaatttgg gctcgaaccc attcaaccag aaggctttcg tgttcacggc
      361 ggatcgcacg ttcggcgcca tgggtccgga ttggaatcag gccaagtaat gaatccaggt
      421 aaaatcgggt gagggcgccg gtatgaaccc gcacccgcaa atctttttca gccagttgtg
      481 gcaggatcag catcggcacc aggaccgcca ccacggctac cgtcatggcg aggacggtgc
      541 ttgacggatc aagccagata attccagccg tggtcatcac aagctggctc atagcctgga
      601 taaattgccc gccaatgtgc ggtaacactc tgattccttg aatgctatga ctgcgttcgg
      661 ccatatctga cgtgaggcgg ctttgaaaat aacggtcgcc caggcggggg attttttcca
      721 gaaacgcgac tctcaagcgg atttcaagat gccgtcccaa tcgaagcatg gcggtcataa
      781 tcggcaaatc cagaaacatc gaaagcgcca aaaagccaag caacgcggcc agtgccacca
      841 ggcgttgttc gggaagggta agctggccgc caatgtcaaa gagtccgcga aacaacaggg
      901 cttcaatgac ggtgcccgcc gccgagcaca tcaaggcgaa ccccaccacc accggtgtca
      961 ggatcccatc ctgggcgagc aaccgcagca gttcacgtcc gggtcgagtg ggtgattcat
     1021 ttaatgccgc aaccagttcg ggcgagaggg caggccgacc ttctgcttga tcctgggctg
     1081 ctttgccagc catttgacgc gaggcccggc agcctttgac ccggaccaga acggcgcctc
     1141 gcatatacag ttgctgattt cccggcgccg ctcgcacaaa ccagtaagca tttggaattt
     1201 ccagcgtgcg gtcttgtgat tcccctgtcg cctgctcaaa aaatgattcc aggacgcggc
     1261 gggcctgttt cccgcgctgg attcccccgg cctgtaccag cgaagtcacc atccgggtgg
     1321 cggcatccag ggcgccaagt gtttgccagg ttggatcagc cagggccgtg gcaaccagtt
     1381 tttcaacacc ggatacgggc caccccaggg tctgtaaacg ctgtttgagt gggttgagga
     1441 aatcagtggt gccagcccat tttcgccagg ccgtggct
//
LOCUS       DAOQDO010001909         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100034_length_1478_cov_1.771609, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001909 DAOQDO010000000
VERSION     DAOQDO010001909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100034_length_1478_cov_1.771609"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1478)
                     /locus_tag="PKZ53_23730"
     CDS             complement(<1..>1478)
                     /locus_tag="PKZ53_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ43515.1"
                     /translation="VLKGSGQIVYGPVTVGGVVNYVTPMPPTRPGGSLMLTGGNRDYF
                     NGIASYGGTWRNTGFLVDYMRKQGEGSRVNLRHKLNDLNVKLVTTLNARNFLTFKANY
                     YGEDSQVTYSGLTLKEFQDDPRQNPFQNDAFEIDRFGFSGTHNFLITSNFVMNTNLYG
                     SIFKRDWWRQSSNSTQRPNDAADPDCGGLQNLNTDCGNEGRLREYYTWGVAPQFRLSH
                     RLFGIRNEADFGFRAHFETQDRLQLNGDSPRARTGIVVEDNIRKNKAFSGYIQNRFII
                     GKFAVTPGVRIEEINYKRTNNLANAGAGITGTTELTQVIPGLGVSYTVNDRTTLFAGV
                     HRGFAPPRTEDIINNTTGGAIELDPGLSWNYEAGIRSLPIQGLRLDAAYFRMNYENQI
                     VPASLAGGSGTTLTNGGETRHQGIELNSRLDTGTLFRSEHNFFVRFNYTYVGIAEFVG
                     KRFSNIPGFSTVSVSGNRLPYSPNHLATTGFGYSHQKGIDVFV"
BASE COUNT          367 a          387 c          361 g          363 t
ORIGIN      
        1 acaaatacat cgattccctt ttggtgtgag tatccaaacc cggttgtggc cagatggttt
       61 ggcgagtatg gcaaccgatt gccggaaaca ctcacggtgc taaatccggg aatattgcta
      121 aaccgtttgc cgacaaactc agctatgcca acataggtat agttgaaccg aacaaagaag
      181 ttatgctcag accgaaaaag cgttcctgta tccagccgac tattcaattc gataccttga
      241 tgacgcgttt caccgccgtt ggtcaaagtc gtccccgaac caccagccag actggctgga
      301 acaatctggt tttcataatt catccgaaaa taggcggcat ccagccgaag cccctgaatg
      361 ggaaggctgc gaatcccggc ttcataattc cagcttaatc ccggatccaa ctcaattgcc
      421 ccgccagtcg tgttattgat gatgtcttcg gttcgcggtg gggcaaaccc acgatgcact
      481 ccggcaaaca gagtcgtccg gtcattgacc gtgtacgaca cccccaggcc aggaatcacc
      541 tgagttagtt ccgtggtgcc ggtgattcca gcaccggcat tggccaggtt gttggttcgt
      601 ttatagttga tttcttcaat gcgtaccccc ggcgtcaccg caaatttgcc aatgataaac
      661 cggttctgga tatagccgga gaatgccttg tttttgcgga tgttatcttc aacgacgatt
      721 ccagtgcggg cccgcggcga gtcaccattg agttgcaacc gatcctgggt ttcaaagtgg
      781 gcacggaagc caaaatcagc ttcgttgcgg atcccaaaca gccgatgact caaccgaaac
      841 tgaggggcaa ctccccaggt gtaatattca cgcagacggc cttcattgcc gcaatcggtg
      901 ttcaggtttt gcaatccgcc gcaatccggg tcagccgcat catttgggcg ctgagtggaa
      961 ttgctcgatt gtcgccacca gtcacgttta aaaattgatc cataaagatt tgtgttcatc
     1021 acaaaattac tggtaatcag aaagttgtgg gtgcctgaaa acccaaaccg gtcaatttca
     1081 aaagcgtcgt tttggaacgg attctggcgc ggatcatcct gaaattcttt gagggtcaac
     1141 cccgaatagg ttacctgtga gtcttcacca tagtaattgg ctttgaaggt caaaaagttg
     1201 cgggcgttca aggtggtgac cagcttcaca ttcagatcat tgagtttgtg gcgcaaattg
     1261 acccgcgagc cttcgccttg cttgcgcata taatcaacca aaaaaccagt attcctccag
     1321 gttccgccat agctggcaat cccgttgaag taatcacggt ttcctccggt caacatcaat
     1381 gacccacctg gtctggtcgg aggcatgggg gtgacatagt tgaccactcc gcctacagtg
     1441 accgggccat agacgatttg accagaaccc tttagaac
//
LOCUS       DAOQDO010001910         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100085_length_1477_cov_2.893812, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001910 DAOQDO010000000
VERSION     DAOQDO010001910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100085_length_1477_cov_2.893812"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1329
                     /locus_tag="PKZ53_23735"
     CDS             385..1329
                     /locus_tag="PKZ53_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNJ43516.1"
                     /translation="MAGTSLLALIDDIASVLDDISVMTKVAAQKTAGVLGDDLALNAQ
                     QVTGVHADRELAVVWAVAVGSLKNKAILVPAALAISAFAPWGITPLLMIGGAFLCFEG
                     FEKLAHKFLHSHAEDEAHHTEHVKALADPAIDLVALEKEKIAGAVRTDFILSAEIIAI
                     TLGTVEKVAFGTQVAVLVGIALIMTVGVYGLVGGIVKLDDAGIYLNKQSGTFQRRLGQ
                     WILLGAPYLMKGLSIAGTAAMFLVGGGILVHGIPGSHDLIHIVEHHAHGIPVVGGILA
                     KIVPMLLDAVIGIIAGALILGCVTLIGKVMKGKKKPSQ"
BASE COUNT          358 a          358 c          387 g          374 t
ORIGIN      
        1 ggttgatacc gaagatggat ggaaaatgaa ggccagcacc accattgaag aaaccctcac
       61 ccttgacgga aagccaatgc aataagcatt agggctgaag aaaatgggct gaaggcttcg
      121 ggctgaagca ggcgggctga agaaagcggg ctgaagaata tcgggctgaa gaaagcgggc
      181 ttggggtatt gaacccaacg ttttcagccc aatgtcttca gcccaatgtc ttcagcccaa
      241 tgtcttcagc tcgatattct tcagccctgg atttttcagc cccaacatgg cttgaccgtt
      301 tgcgaaatcc atggtttttt gacggccttt gcacacattc cctttcattc aattttctat
      361 tcaaccagca gaggaattta tccaatggcc ggaaccagtc tgttagccct gattgatgac
      421 atcgcttcgg tacttgacga tatttcggtg atgaccaaag tcgccgccca aaaaaccgct
      481 ggtgtgttag gtgatgacct ggctttgaat gcccagcagg tgaccggagt ccacgctgac
      541 cgcgaactgg ccgtggtgtg ggccgtcgcg gtgggctcac tcaaaaataa ggcgattttg
      601 gtgccagcgg cgcttgccat cagcgccttt gcaccgtggg gtattacacc gctgctgatg
      661 attggcggtg catttctttg ttttgaagga tttgaaaaac tggcccataa gtttctccac
      721 agccacgctg aggacgaagc ccaccacact gaacacgtca aagccctggc tgatcctgcc
      781 attgatttag ttgcgcttga aaaagaaaaa atcgcgggtg ccgttcggac agactttatt
      841 ttatcggctg aaattatcgc cattacattg ggaacggttg aaaaagttgc gtttggcacc
      901 caggtcgcgg ttctggttgg gattgccctg attatgacgg ttggcgttta tgggttggtc
      961 ggaggcattg tcaaactcga tgatgccggc atctatctga acaaacaatc cgggacattt
     1021 cagcgccgcc ttgggcaatg gatcttgctg ggcgctccgt acctgatgaa aggactctca
     1081 attgccggta ccgccgcgat gtttctggtc ggcggaggca ttctggtcca cggcattccc
     1141 ggttcgcacg acctgattca tatcgtcgag caccacgccc acggcattcc agtggttggg
     1201 ggaattctgg ctaaaatcgt ccccatgctg ctggatgcag tcatcggtat catcgccggg
     1261 gccttgatcc tgggatgtgt cacgctgatc gggaaagtaa tgaaggggaa gaagaaacca
     1321 agtcagtaaa ctggtgttag cttgtcacca aaaagcttac gccagatgaa aactatatga
     1381 cctttgatat tctttctgaa agaaagtctg acctgattca ccacatcaaa cactttgttt
     1441 ggtccgggtt ttattctaaa aaacaaatcc tccatat
//
LOCUS       DAOQDO010001911         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100095_length_1477_cov_2.706048, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001911 DAOQDO010000000
VERSION     DAOQDO010001911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100095_length_1477_cov_2.706048"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..161)
                     /locus_tag="PKZ53_23740"
     tRNA            complement(90..161)
                     /locus_tag="PKZ53_23740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Thr,seq:ggt)
     gene            complement(286..360)
                     /locus_tag="PKZ53_23745"
     tRNA            complement(286..360)
                     /locus_tag="PKZ53_23745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Gly,seq:tcc)
     gene            complement(429..516)
                     /locus_tag="PKZ53_23750"
     tRNA            complement(429..516)
                     /locus_tag="PKZ53_23750"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Tyr,seq:gta)
     gene            complement(628..700)
                     /locus_tag="PKZ53_23755"
     tRNA            complement(628..700)
                     /locus_tag="PKZ53_23755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(665..667),aa:Thr,seq:tgt)
     gene            1058..1279
                     /locus_tag="PKZ53_23760"
     CDS             1058..1279
                     /locus_tag="PKZ53_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43517.1"
                     /translation="MRIPPNIRPIFLIAFIIFALLGSWQYFGYRDNKEANLHFWVSVA
                     LFVVAMISFLIFLLSKPDEDPSDISITKF"
BASE COUNT          363 a          352 c          347 g          415 t
ORIGIN      
        1 caattgttcc cacattgata tgtggcttac tgcgatcaaa tttctctttt gccatggatg
       61 gtttttcgtc ctcgcttaac caagcataaa gcccacaacc gggcttgaac cggtgacctc
      121 caccttacca aggtggcgct ctaccactga gctatgtggg catggattga tattaaatta
      181 ttgaactggc cgtcggagct gttctcaaga ggtgtttcct gagaaggatt cacttcgacc
      241 ggcagcatag attgaatgct gccctgggat acctgagaat caggctggag cgggagacgg
      301 gattcgaacc cgcgaccaac ggcttggaag gccgtgactc taccactgag ttactcccgc
      361 tcgggaaaca gaaacttcaa aattttcaaa aatgaggttt gaaaatttta aactgataga
      421 ttcggtattg gtgcaggggg taggattcga acctacgtaa ggcgtcagcc tgccgggttt
      481 acagcccggt gccattaacc actcgaccac ccctgcacat caattctcaa agaacacgcc
      541 aaaatcaagc tctcattggt gacgtgacgg tctttcaaca agaaaatttt cccaaatcgc
      601 cccagttcat caggtgtgtc tcgatgaagc cggtgaaggg acttgaaccc ccgacctact
      661 gattacaagt cagttgctct accaactgag ctacaccggc atatcgtttt taagctgtat
      721 gagcactgca gcacagttga agtaggggtg ctctcggaaa agtgagcata ctagcagaaa
      781 aattgatcat tgcaaggctt ttttgccagg gattttcaaa tcaaatgcct ggataggtaa
      841 aatgggctgg gaacctggct ctggaaaagc tggtttctga gaaaaagtcg gtggcggttc
      901 ataaagggca ttttcttttg tggtgctctg tggtatctct caggggaaca aggaatccac
      961 agtcagcaca gtagttgtca atcacccaaa acatttaaat cacacacttt ttgtgaagcc
     1021 tgcgatctcc tcattccagg cttcggagga ggccactatg cgtattccgc ccaacattcg
     1081 tcccattttc ctgattgcct ttatcatttt tgccttgctt ggctcgtggc agtattttgg
     1141 gtatcgagat aacaaggaag ccaacctgca tttttgggtt tcggtggcgt tgtttgtggt
     1201 tgcaatgatt tcgtttttga tctttttgct gagcaaaccg gacgaagatc cgagcgacat
     1261 cagcatcacc aaattctgat ctcttcacgt tgaatctcca cctcttttca ctcaatttct
     1321 gggaatggat gccaggcaca ccacagcgtg ggttcctgct ggcactacat tcccagtgaa
     1381 gtgtgtattg cccggattac agttgttgtt ttctcatgat gtttgatacc aagctgggtt
     1441 tacagcgttc atctctcgat tcttcattat ccctcac
//
LOCUS       DAOQDO010001912         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100132_length_1477_cov_2.175809, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001912 DAOQDO010000000
VERSION     DAOQDO010001912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100132_length_1477_cov_2.175809"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..>1477)
                     /locus_tag="PKZ53_23765"
     CDS             complement(72..>1477)
                     /locus_tag="PKZ53_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43518.1"
                     /translation="AVAARREIKDTLPQNQWLDLYAEAFPDKVEQLWQPLGAGPTDEE
                     SPVARLARAWVKRVGRKVAVPVELMAQATAAFEHLPVKPALILEAIVNPSQSGLLTKD
                     TEFYFNEAAYLTSKIVAQAQPADADPDEIEEEDLQPKQNQYFHFGVATTLVNYVPFLY
                     DALPVGDPFRASLPEVLDLVIKRLQNPDLLIALGDKHINVREQNLEKVQSGVDAFLNA
                     IGGDTYTTPDVPAEKMPKGRDNGALVAIIRHLSDYYASISIAYRPSKVTDFAQLQKLI
                     ESPDFYAQERQPVTELLFSAGYRAIIERIKSTPVPVGQWECNPKYSVPELVQDVQQTL
                     NLSENAAILYLQTLALAEPTTKNIQKWNGWTAAQYKKAADELVKANLVLEAKRERAGR
                     AHFLPGGWTALKAPHLPIETWKLALFGVERIKVPQGVQTVVKLRKLFPLRPYHQLFAE
                     VWERIKQGDKPDYEEVE"
BASE COUNT          305 a          388 c          398 g          386 t
ORIGIN      
        1 aacccgtttt cttcagcccc gagcttgcga gtcttcagcc ccaagcccgt ggccttcagc
       61 gccggctttc atcactcgac ctcttcatag tctggtttgt cgccttgttt gatgcgttcc
      121 caaacttcgg caaacagttg gtgataggga cggaggggaa acagttttct gagtttcacc
      181 acagtttgaa ccccctgggg aaccttgatc cgttccacac caaacagtgc cagcttccag
      241 gtctcaattg gcaggtgtgg cgctttgagg gcagtccacc caccagggag aaaatgggcc
      301 cgtccggcgc gttcacgttt ggcttcaagt accagattgg ccttgaccag ttcgtcagcc
      361 gctttcttgt attgggccgc cgtccagcca ttccattttt ggatattttt ggtggttggt
      421 tcagccagtg ccagggtttg cagatacaga atagcggcat tttctgaaag attgagtgtt
      481 tgctggacgt cctggaccag ttccggcacc gaatatttgg ggttgcattc ccattgaccg
      541 accgggacgg gtgtggattt gatgcgttcg atgatggctc gatacccggc tgaaaagagc
      601 aattcagtga ctggttggcg ctcctgggca tagaagtcgg gggattcaat cagtttttgg
      661 agctgggcaa aatccgtaac tttgctgggc cgataggcga ttgatatcga agcataatag
      721 tcgcttagat gccgaatgat ggcgaccagg gctccattat cgcgcccctt cggcattttc
      781 tccgccggga catcgggggt tgtgtacgtg tcgcccccaa tcgcatttaa gaacgcatcc
      841 accccagatt gaactttctc caggttctgt tctctgacgt tgatatgttt atcccccaag
      901 gcaatcagca aatcgggatt ttggagccgc tttatcacca ggtccaggac ctctggtaac
      961 gaagctcgaa aagggtcgcc caccggaaga gcgtcataca aaaatggcac gtaattgacc
     1021 agcgttgtgg ccactccaaa atggaaatat tgattttgct ttggttgcag gtcttcctct
     1081 tcaatctcat ccgggtcagc gtcagcgggc tgggcttgcg ccacgatttt tgaagtcagg
     1141 tatgcggctt cattgaaata aaactcagtg tctttggtca acaaccctga ttgtgacgga
     1201 ttgacaattg cctccagaat cagagccggt ttgaccggaa ggtgttcaaa agccgccgtg
     1261 gcttgagcca tcaactcaac cggaaccgcg acttttcgac caacccgctt cacccaggct
     1321 ctggccagac gggccaccgg gctttcttca tcagttggtc cggccccgag tggttgccag
     1381 agttgttcca ccttgtctgg gaaagcttcc gcatagaggt caagccactg gttttgtggg
     1441 agcgtgtctt tgatttcgcg acgggcagca accgctt
//
LOCUS       DAOQDO010001913         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100219_length_1476_cov_3.292752, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001913 DAOQDO010000000
VERSION     DAOQDO010001913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100219_length_1476_cov_3.292752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..>1476)
                     /locus_tag="PKZ53_23770"
     CDS             complement(197..>1476)
                     /locus_tag="PKZ53_23770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43519.1"
                     /translation="SISKIEAGKLTLKNAIFDLPALVQGLSEMFYFRAQSKGITLDVR
                     MDPGLPHYVSGDEGKLRQVLINLIGNAFKFTEFGRVVIRVKPLDEELCHFEVEDTGCG
                     IAENDLEQLFEPFVQINSPGRKNQEGTGLGLTISRNYVRLMGGDLTVRSIPTQGTSFS
                     FWLPLPSLPSSEYTVPISHSVIGLEDGQPHYRILVVEDQDDNRALLCRLLKSAGFEVY
                     EAIDGKRAIEGWARYRPHLIMMDLHMAVLDGYEAMRIIRAIESGAGQDILPTSVDVTL
                     PEEIVRTKIIAVSADAFGVDRSRIREFGGDDFIPKPFHVEVIFEKLREHLSVKFVQIN
                     RTTGQLIAPAGLLNQTLTPDRFQPFLKEWLVRMHHSVQSGDVDEAARLIEDIAPIDTE
                     LAFELKKLLRSYDFDELIDALVVAIEQHGQNFQ"
BASE COUNT          402 a          389 c          329 g          356 t
ORIGIN      
        1 aactctctcc gattcatgca gttctctcct taactgaaaa caaattaatg taaacgatgg
       61 ttgccaaagc gagcagcttt gtacagggat tgctctttgc aaggttgact ttcgaatgga
      121 ttttttagaa gcgcgtctat ttgacaaaag tgaccaggaa aaatcaagtc taaatcattg
      181 aacgacaaac ttttggtcat tgaaaatttt ggccatgttg ctcaatggcg accacgaggg
      241 catcaatgag ttcatcaaag tcataactgc gcagcaattt cttcaattca aaggccagtt
      301 ctgtgtcaat gggggcaata tcttcaatca accgggcagc ctcatcaaca tcacctgact
      361 gaaccgaatg atgcatcctc acaagccatt ctttcaaaaa gggttggaag cgatctggag
      421 tgagagtctg attaagaagt ccagccggag caatcaattg tccagttgtc cgattgattt
      481 ggacaaactt cacactgagg tgctcgcgca atttttcaaa aatcacctcg acgtgaaagg
      541 gtttggggat aaaatcatcg cccccaaatt cgcgaatgcg tgaccgatcc acaccaaagg
      601 catcagccga gacagcgatg attttggtcc gcacgatttc ttcaggcagg gtgacatcca
      661 cactggtggg taaaatatcc tggccggcgc cgctttcaat ggctcgaata atccgcatgg
      721 cttcataccc atccaaaacg gccatgtgca aatccatcat gatcaggtgt ggacgatacc
      781 gggcccaacc ttcaatcgcc cgcttcccat caatggcttc atagacttca aacccggcag
      841 atttcagcaa ccggcacagc agggcgcgat tgtcgtcctg gtcttcaacc accagaatcc
      901 gatagtgggg ttgaccatct tcgaggccaa tcacggaatg ggaaatcgga accgtatatt
      961 cactgctcgg cagacttggc agcggcaacc agaaactgaa actggtgccc tgggtgggaa
     1021 tgctccggac ggtcaggtca ccgcccataa gtcgaacata atttcgcgaa atggtcagcc
     1081 ctagccccgt accttcctgg tttttgcgac cagggctgtt gatctgcaca aatggttcaa
     1141 ataactgctc cagatcattt tccgcaattc cacacccggt atcctcaacc tcaaaatggc
     1201 agagttcttc gtccagcggc ttaactcgaa tgaccacccg cccaaactca gtaaatttaa
     1261 acgcattgcc aatcaaattg atcaacacct ggcgcagttt tccctcgtct cccgatacat
     1321 agtgcggcaa cccgggatcc attctcacat cgagggtaat ccctttcgat tgcgcccgaa
     1381 aataaaacat ttccgaaagc ccctgcacca gcgcgggcag gtcaaagatt gcgtttttga
     1441 gggtcagttt cccggcttca attttggaaa tcgaaa
//
LOCUS       DAOQDO010001914         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100248_length_1476_cov_2.725545, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001914 DAOQDO010000000
VERSION     DAOQDO010001914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100248_length_1476_cov_2.725545"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PKZ53_23775"
     CDS             <1..1382
                     /locus_tag="PKZ53_23775"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNJ43520.1"
                     /translation="QSGILSTPAVSCIIRKYQAFGGIILSASHNPGGPHGDFGIKYNI
                     SNGGPAPEKVTEAIFARTKTIAEYRVLDAPDIDLDQLGTSTLGNTTVEVVDSVADYAE
                     LMETLFDFAAIRELVTSGSFRMAIDSMHAVTGPYAREIFEHRLGAPTGTVMNGIPLED
                     FGNGHPDPNLVYAHELVEKLFGEDVPDFGAASDGDGDRNMVLGGNFFVTPSDSLAVLA
                     ANATLVPGYRNGIAGVARSMPTSQAADRVATKLGINCFETPTGWKFFGNLLDAGTATL
                     CGEESFGTGSNHVREKDGLWAVLFWLNILAVRKQSVAEIVREHWQTYGRNYYSRHDYE
                     EVATEPANQLMNQLRAALGEMPGKTPGSYQVEYADDFSYTDPVDGSVSQKQGVRIGFT
                     DGSRIVFRLSGTGTKGATLRVYFEKYEPDTSNHGLDPQTALADLITIADDVAQIRTLT
                     GREKPDVIT"
BASE COUNT          364 a          376 c          409 g          327 t
ORIGIN      
        1 gtcaaagcgg gatcctctca acaccggctg tctcgtgcat tatccgcaaa taccaggcgt
       61 ttggaggaat cattctttcg gcgtcacata atcccggcgg gccacatggc gattttggga
      121 tcaaatacaa catcagtaac ggcggcccgg ccccagaaaa agtaacggaa gctatttttg
      181 cccgtaccaa aacgattgcc gaatatcggg tccttgacgc tccggatatt gatcttgacc
      241 agttgggaac ctcaacactc ggaaatacaa ccgttgaagt ggttgattcg gtagcagatt
      301 atgccgaatt gatggaaaca ctctttgatt ttgcggccat ccgcgagctg gtcacgtctg
      361 gttcatttcg aatggcaatt gactcgatgc atgcggtaac cggcccgtat gcacgtgaaa
      421 tctttgaaca tcggcttggc gccccaaccg gcaccgtcat gaatggcatc ccactcgaag
      481 atttcggcaa cgggcaccca gatccaaacc tggtctatgc ccatgaactg gttgaaaagc
      541 tgtttggtga ggacgttcca gattttggcg ccgcttccga tggtgacggt gaccgaaaca
      601 tggtgcttgg cggcaacttt tttgtcaccc caagcgacag cctggcggtt ctggcagcca
      661 atgcgacact ggtgccgggc taccggaacg gcatcgccgg cgtggcacgc tcaatgccga
      721 ccagtcaggc agccgaccgg gtcgcgacca aacttggaat caattgtttt gagacgccaa
      781 ccgggtggaa attctttggc aatctattgg atgcgggcac agcaactctg tgtggtgaag
      841 aaagttttgg cacgggctca aatcacgttc gcgaaaaaga cggcctgtgg gcagtgctct
      901 tctggcttaa tatcctggcg gtgagaaaac aatcggtggc tgaaattgtc cgcgaacact
      961 ggcaaaccta tggccgaaat tactattccc ggcacgacta tgaagaagtt gccaccgaac
     1021 cagccaatca attgatgaac cagcttcggg cagccctggg tgaaatgcca ggaaaaacac
     1081 ctggcagcta tcaagtcgaa tatgccgatg attttagcta cacggacccg gtggacggca
     1141 gcgtgagcca gaaacaaggg gtcagaattg gatttactga cggttcgcgg attgtcttcc
     1201 ggctctctgg cactggaacg aagggggcaa cgctccgggt gtactttgaa aagtatgaac
     1261 ctgataccag caaccatggc ctggatccac aaacagccct ggccgatctg attacgattg
     1321 cggatgatgt ggctcagatt cgcaccttaa ccggtcgcga aaaaccggat gtcataactt
     1381 aatttgggtt ccgggttccg ggttccgggt tccgcgcttc cgggttccgg gttttcgaaa
     1441 gggaaaggac aaaaggggcg aaaaggacat aaatgc
//
LOCUS       DAOQDO010001915         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100507_length_1474_cov_2.892178, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001915 DAOQDO010000000
VERSION     DAOQDO010001915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100507_length_1474_cov_2.892178"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1268)
                     /locus_tag="PKZ53_23780"
     CDS             complement(6..1268)
                     /locus_tag="PKZ53_23780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43521.1"
                     /translation="MHALLENLILAISTIFSNKLRSLLTVLGVVVGTATVIAVSSVLT
                     GLRVRTQELAEQTGPNVIYVSKYTNIGPRFSRPSPEERQRKPLVLEDAVAINELPAVQ
                     AASPQLVVGSFGPSATQLNVKYQGREAVRPIIFGVWANYPTVRRTDLRSGRFFTSQEV
                     EHKLNVVVIGPAIADTLFADESPLEKEIEVEGQLYRVIGVVEKGPTGIFGDTAEDRQV
                     LIPYDSLAKRYADILNDRGITIIACAQDGKLDQMRDEITELMRRRRAVRYDQPDNFGL
                     NTPDAIFSTFDNITTILGLIVVPISGAGLLVGGVGVMNIMLVSVTERTREIGVRRAIG
                     ARKLDILSQFLIEAVSLTVTGGGIGILIGFAISLSLNTFMPTVPSVVPLWSVIIGFGI
                     SVAVGILFGLWPAMRAARLDPSEALRYE"
BASE COUNT          384 a          375 c          365 g          350 t
ORIGIN      
        1 ggtacttact cataccgaag tgcttcagac gggtcaaggc gagcagcacg catcgcgggc
       61 caaagcccaa ataaaattcc tacggcaact gaaattccaa acccaatgat cactgaccag
      121 agtggtacca cggagggcac tgtcggcata aaggtattga gactgaggct gatggcaaat
      181 ccaatcagaa ttccaatccc gccaccagtc accgtgagtg aaaccgcttc gatcagaaac
      241 tgggataaaa tatcaagttt gcgagcccca attgcccggc gaactccgat ttcacgggtg
      301 cgctcagtga ccgaaaccag catgatgttc atcacgccaa cacctccgac cagaagcccg
      361 gcaccggaaa tcggcaccac aatcagcccc aaaatcgtgg tgatgttgtc aaaagttgag
      421 aaaatagcat ctggcgtgtt gagaccgaaa ttgtcaggct gatcatatcg aacggctcgc
      481 cgccgccgca tcaactctgt aatttcatcc cgcatctggt ccagtttgcc atcctgggca
      541 caggcaatga tggtaatgcc acggtcgttc aaaatatcag catagcgttt ggccaaacta
      601 tcataaggaa tcaaaacctg ccggtcttca gccgtatcgc caaaaatgcc ggttggacct
      661 ttttcaacca ccccaatcac tcgatagagt tgcccttcga cctcgatttc cttttccagt
      721 ggtgattcat ccgcaaataa agtgtccgca atcgcaggcc caatcacaac cacattgagc
      781 ttgtgctcga cttcctgtga cgtaaaaaac cgtccgctgc gcagatcagt ccggcgcaca
      841 gtagggtagt ttgcccaaac cccaaaaatg attgggcgga cggcttcacg tccctggtac
      901 ttcacgttga gttgagtcgc actcggtcca aagcttccaa caaccagttg tggactggcc
      961 gcctggacag ccggaagctc attgatcgca acggcatctt ccaggaccag cggtttacgc
     1021 tgacgttcct caggtgatgg tcggctaaaa cgaggaccaa tgttggtgta tttgctcaca
     1081 tagatcacat tggggccagt ttgttcagcc agttcctgtg ttcgtacacg caaccccgtc
     1141 agcaccgaag acaccgcaat cacggttgcc gttccaacaa caacccccaa cacggtcaga
     1201 agtgaccgta atttgtttga gaaaatcgtc gaaatggcca gaatcaggtt ttcgagaagt
     1261 gcgtgcatag gtaaggaaag aatgaagaat gaagaattga gaatgaagaa tgaagaatga
     1321 agaatgaaga atgaaaaatg aagaatgttc ctgatgtttg gttcttggtg cttggttctt
     1381 ggttcttggt gcttggttcc tgatgtttgg tgcttggtgc ttggttcttg gtgcttggtt
     1441 cttggtgctt ggttcttggt tcttggtgct tggt
//
LOCUS       DAOQDO010001916         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100533_length_1474_cov_2.405215, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001916 DAOQDO010000000
VERSION     DAOQDO010001916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100533_length_1474_cov_2.405215"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1474)
                     /locus_tag="PKZ53_23785"
     CDS             complement(<1..>1474)
                     /locus_tag="PKZ53_23785"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739778.1"
                     /note="catalyzes the reversible formation of D-erythrose
                     4-phosphate and D-fructose 6-phosphate from sedoheptulose
                     7-phosphate and D-glyceraldehyde 3-phosphate; catalyzes
                     isomerization of glucose 6-phosphate and fructose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNJ43522.1"
                     /translation="VMDTLAAIGISMEEVTDTLLSDAVRLFAEPFEKLLRSIDKKCKL
                     VSWTEVNRQSYHLPAELAALVKESIEDWQVNGKVRRLWARDTSLWTRSDENSWLGWLS
                     IIEDQLGDPAHLHRIAEDVKAAGFSHILLLGMGGSSLCPEVFKMTFGHLDGFPELHVL
                     DSTDPAQVKAFEHKVDLAKTLCIVSSKSGSTLEPNIFKQYFFERLKETVGADQAGSHF
                     VAITDPNSKVEQIATSEGFRHIFHGLPSIGGRYSALSNFGMIPAAAMGLDTLKFLDLS
                     EEMASACSHCVPTGDNPGVVLGNVLGALARAGRDKVTIITSPDIWDFGAWLEQLLAES
                     TGKEGKGLIPVDRENLGGPEVYGSDRIFAYLRSDNGADPAQDAAIDALEQAGHPVVRI
                     NLIDTYHIGQEMFRWEIATAVAGSVIGINAFNQPDVEASKIATRKLTSEYEKTGSLPA
                     ESPFFEADGIKLFSDEANVAALTAAVGENQSLTAFVKAHLD"
BASE COUNT          303 a          379 c          466 g          326 t
ORIGIN      
        1 ggtcgagatg agctttgaca aaggcagtga gtgattgatt ttcgccgacc gcggcggtga
       61 gggccgcgac attggcttca tcactgaaga gtttgattcc atcagcctca aagaacggtg
      121 attcggctgg aagtgagccg gttttttcat attctgaggt gagtttgcgg gttgcaatct
      181 tgctggcttc gacgtcgggc tggttaaagg cattgattcc aatgacggag ccggcgacgg
      241 cggtggcgat ttcccagcgg aacatttcct gcccgatgtg ataggtatca atcaggttga
      301 tccgcaccac cgggtgcccg gcctgttcga gggcgtcaat cgcggcatcc tgggccgggt
      361 cggcgccgtt atctgatcga aggtaggcaa aaatgcggtc actgccgtac acctcagggc
      421 caccgaggtt ttcacgatcc actggaatca ggcccttgcc ctccttgccg gtggattcag
      481 ccagcaactg ttccagccag gcgccgaaat cccagatatc aggcgaggta atgatcgtga
      541 ctttgtcgcg accagcacgg gccagggcgc ctaacacatt gcccagcacc acaccgggat
      601 tatcgccggt cggcacacag tgcgagcagg cgctggccat ttcttcggac aggtcaagga
      661 atttaagggt atcgagcccc atggcggcgg cagggatcat gccaaaattc gagagggctg
      721 agtatcgtcc gccaatgctg gggaggccgt ggaaaatatg gcggaagcct tcgctggtgg
      781 cgatctgttc gaccttggag ttggggtcgg tgatggcgac aaagtggctg ccggcctggt
      841 cggcgccaac ggtttctttt aagcgttcaa aaaagtactg cttgaagatg ttgggctcca
      901 gcgtgctgcc ggatttgctc gacacaatgc acagggtttt ggccagatca actttgtgtt
      961 caaaggcttt gacctgggcc gggtcggtgg aatccagcac atggagttcg gggaagccgt
     1021 caaggtggcc gaaggtcatt ttgaagactt cgggacacag gctggagccg cccataccaa
     1081 ggagcaggat atgcgaaaac ccagcggctt tgacgtcttc ggcaatgcga tgcaggtgag
     1141 ccgggtcgcc aagctggtct tcgatgatgc tgagccagcc aagccagcta ttttcatctg
     1201 accgggtcca gagcgaggtg tcacgtgccc agagtcggcg gaccttgcca ttgacctgcc
     1261 agtcttcgat ggattctttg accagggccg ccagttcggc gggcaggtga taggactgcc
     1321 ggttgacttc agtccaggag acgagtttgc actttttgtc gattgagcgg aggagtttct
     1381 caaacggttc ggcaaacagc cggacggcat cgctcaggag ggtatcggtc acttcctcca
     1441 ttgaaattcc aatcgcggcg agggtatcca tcac
//
LOCUS       DAOQDO010001917         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100624_length_1473_cov_3.817348, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001917 DAOQDO010000000
VERSION     DAOQDO010001917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100624_length_1473_cov_3.817348"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1473
                     /locus_tag="PKZ53_23790"
     CDS             <1..>1473
                     /locus_tag="PKZ53_23790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNJ43523.1"
                     /translation="SDAVLTIFKRARRDRTLANTILTTGYPLIFETLPAKFAKFAQPF
                     REIIDQLEVESLVIIALQSKDRVLGVMVFGFRSGQMLAPSEVQLLNAIGRQVGVAVEN
                     AQLISSLQDALKDIREASRLKDEFLATLSHELRTPLTSVTGWSEVLIDRVSQYADAEL
                     QEGISTIHHNAENLKQLINDLLDLSRVENRVLKLELELTNINVAIQSAVQTVKQMAEN
                     KGVHLVMQLSDRLPKINADVNRLQQVFWNLLTNAIKFTEKSGVVCLTSQESPGGVEVS
                     VTDNGIGIESEFLPFVFERFRQADGSSTRRFGGLGIGLSLVKSFTEAHGGSVDVTSKG
                     PGQGSTFFVRLPAAEIVERNLEPERRSGPHQISRITGEVKPLTPVILIIDDLEDSLRM
                     LESTLKREGYSTATANTADAGLRMAERTPPDLILVDMEIPGMGGVEVFRRLQANLKLM
                     QIPVVGILGMGMDSQRTQALELGFRGIVTRPFRRRELLEII"
BASE COUNT          355 a          340 c          406 g          372 t
ORIGIN      
        1 tcagatgcgg tattgaccat ttttaaacgt gcccgccggg atcgaaccct ggcgaatacc
       61 attttgacga ccggataccc gttgattttt gagaccttgc ctgcgaaatt tgcgaaattt
      121 gcgcaacctt ttcgggaaat tattgatcag cttgaagtgg aatcactggt gatcattgct
      181 ttgcaatcca aagatcgggt gctgggggtg atggtttttg gatttcggag cgggcaaatg
      241 ctggcgcctt ccgaagtcca gttgctcaat gcaattggcc gacaggttgg agtggcggtt
      301 gaaaacgccc agttgatttc cagtttgcag gatgcgttga aggacattcg cgaagccagc
      361 cgtctcaaag acgagtttct ggcgacctta tcacacgagc ttcgcacccc gctgacttca
      421 gttactggct ggtctgaggt tttgattgat cgagtgagtc agtatgctga tgccgaatta
      481 caggaaggca tcagcaccat ccaccacaat gctgaaaacc tcaaacagct cattaatgac
      541 ttgcttgatt tgtcgcgggt ggaaaaccgg gtgctcaagc ttgagcttga actcaccaat
      601 atcaatgttg ccattcaatc agccgttcaa accgtcaagc aaatggctga aaataaaggt
      661 gttcacctgg tgatgcagtt gagtgaccgg ctcccgaaaa tcaatgcgga tgtcaaccgg
      721 ctgcaacagg tcttttggaa tttattgacg aatgcgatta aatttactga aaaaagcggg
      781 gtagtgtgcc tgacttcgca ggaatcgcca ggtggtgtcg aagtcagtgt gactgataac
      841 gggattggaa tcgaaagcga atttctcccc tttgtgtttg agcggtttcg ccaggctgat
      901 ggcagttcga cccgacgctt tggcggcctt gggattggcc tctcactggt gaaatcgttt
      961 accgaagccc acggcggcag tgtggatgta accagcaaag gacccggaca gggaagtacc
     1021 ttttttgtcc gactcccagc ggcagaaatt gttgaacgaa acctcgagcc tgagcgacgc
     1081 agtggtcccc atcaaatcag tcgaattact ggtgaagtaa aacccctgac acccgttatc
     1141 ctgattattg atgacctgga agacagttta cggatgctgg aatcaacctt gaaacgagaa
     1201 gggtacagta ctgctacggc caacactgct gatgccggcc tgcggatggc tgaacggacg
     1261 ccgcctgatt tgatcctggt tgatatggag attcctggaa tgggtggagt tgaagtcttt
     1321 cggcggcttc aggccaacct gaagttaatg cagattccag tggttggcat tttagggatg
     1381 gggatggatt cgcagcgaac ccaggccctt gaacttgggt ttcgcggaat cgtcacccgc
     1441 ccattccgac ggcgtgaact tctggaaatc att
//
LOCUS       DAOQDO010001918         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100629_length_1473_cov_3.573343, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001918 DAOQDO010000000
VERSION     DAOQDO010001918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100629_length_1473_cov_3.573343"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /gene="lpdA"
                     /locus_tag="PKZ53_23795"
     CDS             <1..1215
                     /gene="lpdA"
                     /locus_tag="PKZ53_23795"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNJ43524.1"
                     /translation="KHKANQFGIKVDGTLSFDYGEAYRRSRKVADGRVKGVHFLMRKN
                     KIDEYDGWASFQDANTLEVALNKGETETLKFKNCIIATGATTRLIPGSKLSERVVTYE
                     EQILEDKVPGSIIIAGAGAIGVEFAYVLSNYGCQVTIVEFLDRIVPLEDEEISAELKK
                     HYERAGIKIMTSTRVDLIEDTGSNVRVTVTDGTGQQKVLEADKVMQAIGFKPRVEGYG
                     LENTGVKLTDRGAIEISDYMQTNVPHIYAIGDVTAKLMLAHVAEAMGVIAAEHMTGTE
                     TMGLNFVMMPRATYCQPQVASFGYTEKQAIEQGYDVKVAKFPFTANAKAHGLNDAIGF
                     VKLISDKTHGELLGGHLIGPEVTELLPELTLAQQWELTVEEMSRNVHAHPTLCEAVKE
                     AIHGLAGHMINM"
BASE COUNT          431 a          359 c          370 g          313 t
ORIGIN      
        1 aagcacaaag ccaaccagtt tggaatcaaa gtggatggaa cgctcagctt tgactatggt
       61 gaagcctacc gccgtagccg caaagtcgcc gatggacgcg tcaaaggtgt ccactttctg
      121 atgcgcaaaa acaaaattga cgagtacgac ggctgggcca gttttcagga tgccaacacg
      181 ctggaagttg ccttaaataa aggcgaaacc gaaaccctca aattcaaaaa ctgtatcatt
      241 gccaccgggg cgacaacccg tttgattcca ggttcgaagt tgagcgaacg tgtcgtgacc
      301 tacgaagagc aaatccttga agacaaggtt ccaggcagca tcattattgc cggtgctggc
      361 gccatcggag tcgaatttgc ctatgttttg agcaattatg gctgtcaggt gacaattgtt
      421 gaatttttgg atcgaattgt gccgcttgaa gacgaagaaa tttcggctga attgaaaaaa
      481 cactatgaac gtgccggcat caaaatcatg accagtaccc gtgttgatct gatcgaagac
      541 accggctcaa acgttcgggt cacggtcact gacggcacgg gacagcaaaa agtgctcgaa
      601 gccgacaaag tcatgcaggc gattggattc aagccacgtg tggaaggcta tggcctggaa
      661 aacacgggtg tgaagctgac tgaccgtggt gcgattgaaa tcagcgacta tatgcaaacc
      721 aatgtgccgc atatttatgc cattggtgat gtgacggcca aattgatgct cgcccacgtg
      781 gccgaagcga tgggggtgat tgccgctgaa cacatgaccg gaacggaaac catgggcctc
      841 aactttgtca tgatgccgcg ggcgacctac tgccagccgc aagttgccag ctttggctac
      901 acggaaaaac aggcaattga acagggctat gacgtcaagg ttgccaaatt cccattcacc
      961 gccaatgcca aagcccacgg cttaaacgat gccatcggtt ttgtaaaact catcagcgac
     1021 aaaactcatg gcgaattgct cggcggccac ctgattggac cggaagttac cgaactgctg
     1081 ccggaactga ctctggctca acaatgggaa ctgacggttg aagaaatgtc ccgcaacgtt
     1141 catgcccatc caactctgtg cgaggcggtc aaagaagcca tccacggtct ggctggccac
     1201 atgatcaata tgtgagaaat tgagaatgaa gaatgaagaa tgaagagtga agaatgaccc
     1261 agattttgcc caaaatatca ctacattgct caacaatcag tgccgaccag atcgggagac
     1321 gactcgcaca ggggaatccc aatcaacctg aaatggaatg caaattcact aaaatttcct
     1381 cgtcacttcc accaaactga ctccgattaa gtcctttctg tcctttgatt gtggtctcat
     1441 caggtaccaa aatcacaagg acaaaaagga caa
//
LOCUS       DAOQDO010001919         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100764_length_1472_cov_3.705011, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001919 DAOQDO010000000
VERSION     DAOQDO010001919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100764_length_1472_cov_3.705011"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKZ53_23800"
     CDS             <1..1088
                     /locus_tag="PKZ53_23800"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ43525.1"
                     /translation="WLIKFYQNLPLKNIRHEANKNTLSRRSFMNFKRFNFIVGLMLIT
                     MIVLAACGTSSAAKPADKITVQLSWFHGVEYAGFYTAVEKGYYAEENIEVILNAGGPE
                     INPVNEVGNGNAQLGITSGDSIIIEKSNQKNFIAVGTIFKENPLTITSLSKDNIQKPN
                     DLVGKTVGVYSLDLSNFFDLPFLALLSRTGLDRDSIQYALIQDFQGANEIKAGNMDAM
                     SGMFATDQQVMAMEAGDELSLIYYKDYGYDVYINTIFTTSEYAANNPDILTRFMRATM
                     KGYQYAIENPEEAASLALKYDPALDLGYQTEVMKVQIPFIDTGDGPLGSMNESIWKNT
                     QDILLEFDLISSPVDLNSIYTNQFVTP"
     gene            1126..>1472
                     /locus_tag="PKZ53_23805"
     CDS             1126..>1472
                     /locus_tag="PKZ53_23805"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ43526.1"
                     /translation="MKKNSFRYGFILLAVMALFINACAGGNPFQENDKVTVQLSWFPS
                     VEYAGFYTALEKGYYSQENLDVTLVAGGPELDVLGEVNSGNAQFGIGTGDSLIIAKTR
                     QKNFLAVATIFRN"
BASE COUNT          434 a          327 c          333 g          378 t
ORIGIN      
        1 cctggctgat taagttttat caaaatttgc cattgaaaaa cattagacat gaagcaaaca
       61 aaaacacatt atcaaggaga tctttcatga atttcaagag atttaatttt atcgttgggc
      121 tgatgttgat cacaatgatt gtcttggctg cttgcggaac ttccagcgct gccaaacctg
      181 ccgataaaat cacggttcaa ttaagttggt tccatggcgt tgaatacgcc gggttctata
      241 cggctgtgga aaaggggtat tacgcggaag aaaacatcga agtcattctg aatgcaggtg
      301 gcccggagat caatccggtc aatgaagttg ggaacggaaa tgcccaattg gggatcacca
      361 gcggcgacag cattattatt gagaaatcca atcaaaagaa ctttattgct gtgggaacaa
      421 ttttcaaaga aaacccgttg accatcacat ccctgtccaa ggataacatc caaaaaccaa
      481 acgacctggt cggaaagact gtgggtgttt actcgcttga tctaagcaat ttcttcgatc
      541 tgccgtttct ggctttgtta agccgcactg gactggatcg tgattccata caatatgccc
      601 tcattcagga ttttcaggga gccaatgaaa tcaaggccgg taatatggat gccatgagcg
      661 gcatgttcgc caccgatcaa caagtgatgg ccatggaagc cggagatgaa ttgagcctca
      721 tttattataa agattatggg tacgatgttt atatcaacac cattttcacg acaagtgaat
      781 atgccgccaa caaccccgac atactgaccc ggttcatgcg cgcgaccatg aaggggtatc
      841 aatatgccat agaaaatccg gaagaagctg cctcgctggc tcttaaatat gaccctgctc
      901 tcgatctcgg atatcaaaca gaagtcatga aagtgcaaat cccttttatt gatactggtg
      961 atggtccgtt gggctcgatg aatgaaagca tctggaagaa tactcaggac attcttcttg
     1021 aattcgacct gatctcatct ccagtcgatc taaacagcat ttataccaac caatttgtaa
     1081 caccttagcc attcgtacca aggaagacct cataaggagc acgaaatgaa aaaaaactct
     1141 ttccgctatg gtttcatctt actggctgtc atggcattgt tcatcaatgc atgtgctggt
     1201 ggcaatccgt tccaggaaaa cgataaggtg actgtccagt taagctggtt tcccagtgtt
     1261 gagtacgccg ggttttatac cgccctggaa aaaggatatt actcacaaga aaaccttgac
     1321 gtaaccctgg tcgcaggcgg gccggagctt gacgtgcttg gcgaggtcaa cagcggaaat
     1381 gcccagtttg gcatcggtac cggagatagt ttgatcattg ccaaaacaag gcaaaaaaac
     1441 ttcctggctg tcgccacaat cttccgaaat aa
//
LOCUS       DAOQDO010001920         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_100909_length_1471_cov_3.033192, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001920 DAOQDO010000000
VERSION     DAOQDO010001920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100909_length_1471_cov_3.033192"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="PKZ53_23810"
     CDS             complement(<1..1429)
                     /locus_tag="PKZ53_23810"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNJ43527.1"
                     /translation="MIGITWHPSTTGRVPVELDVSCFLLKPDGKVRSDADFIFYNQNK
                     DLAKSVFLLTEGDSALPQGAAIGFQAILNKIPASISKLAFCLTIYEATPRRQSFGQVK
                     GIRIFLTNRAGTLLGQYELSNHLTSETAVILGELYRYKDQWKFRAVGQGYTGGLATLA
                     TGFGVAFDAPTHPTPAVAPPRSNQPAAQISTPPVPQPPRSNQPAMARQIPAVQAPPPP
                     VPQPQVFPDGFCQRCGKEAGLIGKLFGYNAQTKRCKACDKEYQKELNVFRQYFIQYCA
                     DGILTQNEWANLQRIATDRRLKWDEALGYVQADSVLFLERTLTFAASDGIITDEEYTG
                     IHQLQKKLNIPLVQAKPIFERLDSLKRVSDIRQGKLSACTTNAHLESGEVCYLETSAV
                     YHKITTRTTEQVPGTFVATNKILRFLSPKGGWTIQWKNVMRTSQGNQCVCLELSTKKG
                     NGHYQVADCVYVEAVIAALTKIAKRQ"
BASE COUNT          358 a          364 c          378 g          371 t
ORIGIN      
        1 gctggcgttt ggcaattttg gtcagggccg caatcacagc ttcgacatac acacaatctg
       61 ccacctggta atgaccattt cctttcttgg ttgaaagttc aaggcacaca cactgattgc
      121 cctgtgacgt ccgcatcacg tttttccatt gaatggtcca gccacctttg ggcgagagaa
      181 atcgaaggat tttgttggtc gccacaaagg ttcctggaac ctgctcggtc gttcgagtcg
      241 tgattttgtg atacaccgca ctcgtttcca ggtagcacac ttcacctgac tcaaggtgtg
      301 cattggtggt acaggcggaa agttttccct gccggatgtc agacacccgc ttcaaagaat
      361 cgagtcgctc aaagattggc tttgcctgaa ccagtggaat gttcaatttc ttctgaagtt
      421 gatggattcc ggtatattcc tcatcggtaa taatgccatc tgacgccgca aaagtaaggg
      481 ttcgttctaa aaagaggact gaatccgcct gaacataccc cagagcctcg tcccacttaa
      541 gtcgccgatc agtcgcgatc cgctgcagat tggcccattc gttctgagtg agaattccat
      601 cagcacaata ctgaataaaa tactgccgaa aaacattgag ttctttctgg tactctttgt
      661 cacaggcttt acagcgcttt gtttgggcat tgtagccaaa gagcttgccg atgagtccgg
      721 cttctttccc acaccgctgg caaaatccat ccgggaatac ctgcggctgc ggaactggag
      781 gcggtggagc ttgaacagcc gggatttgtc gagccatcgc cggttgattt gaacgcggtg
      841 gctgcggaac tggaggcgtt gaaatttgag ccgccggttg atttgaacgt ggcggagcaa
      901 ctgccggagt cgggtgtgtc ggtgcgtcaa atgcaacgcc gaacccggta gccagggtgg
      961 ccagtccgcc tgtatatccc tgaccgacgg cccgaaattt ccactggtct ttgtaccgat
     1021 acagttcgcc cagaataacc gctgtttcac tggtgagatg attggacaat tcatactgac
     1081 cgagaagcgt tccagcccga ttggtcaaaa aaatacgaat ccctttcacc tgaccgaaag
     1141 attgtcggcg aggagttgct tcataaatcg tcaggcaaaa tgccagcttt gaaattgaag
     1201 ccggaatttt gttcagaatg gcctggaatc caatcgccgc tccttggggg agagccgaat
     1261 caccttcggt cagtaaaaag accgatttgg ccagatcttt gttctggtta tagaaaatga
     1321 aatcagcatc ggagcggact tttccgtctg gtttgaggag aaaacaactg acatcaagtt
     1381 caaccggcac ccggccagtc gttgacgggt gccaggttat tccaatcatt acagtgggag
     1441 aacgttcgtc agacggttcc aaaagaaaat t
//
LOCUS       DAOQDO010001921         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101046_length_1470_cov_2.886926, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001921 DAOQDO010000000
VERSION     DAOQDO010001921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101046_length_1470_cov_2.886926"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1470)
                     /locus_tag="PKZ53_23815"
     CDS             complement(<1..>1470)
                     /locus_tag="PKZ53_23815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ43528.1"
                     /translation="QKEYSETIYRSGEALLLIINDILDFSKIEAGKLEVEQSPFELQP
                     VLEGVMDLLAESAQRKQLEFTCVVSPTVPQILCGDPARLRQVLTNLLSNGIKFTDQGE
                     VTLRVSLVNQSTGQVRLRFEIKDTGIGISPEQQSLLFQPFTQADSSNTRRFGGTGLGL
                     VISKKLIEAMQGEVQFESQMDQGTTFWFEIEFPVQAQTPPVWELPQSVSNRRILVVED
                     HPPCLELITQMLATWHLPFDQATTAESVLDKIQEAELTTTPFDTIILDITQPGLDISI
                     LIETLPHKLISEKNLILLAPWGQSQFVQLPNQFKAVRYLQKPIRLRQLFESLFPENQL
                     AGVGASTSYSVESADSTSLFAGCQVLVVEDNLINQRVTLYQLLRFGVEVHIANNGREA
                     LAALQKSSYDLVLMDCQMPEMDGYEATRQYRQCEATGQHLPIIALSANAYSENKHRCL
                     QSGMDDFISKPVKLPELERVLKQWIIKAKPALPALPVSQP"
BASE COUNT          354 a          343 c          403 g          370 t
ORIGIN      
        1 ggttggctga caggcagcgc aggcagggct ggtttggctt tgattatcca ttgtttgaga
       61 acacgctcta gttctggaag cttgaccggt ttcgaaataa aatcatccat tccagactga
      121 agacaccggt gtttgttttc agagtaggca ttggctgaca gggcaatgat cggcaaatgc
      181 tgtccagttg cttcgcattg gcggtactga cgggttgctt cgtatccgtc catttctggc
      241 atctggcaat ccatgagaac cagatcgtag ctggattttt gcaaggcggc cagggcctct
      301 cggccattgt tggcgatatg aacttcaacc ccaaaccgca ggagctggta aagcgtgact
      361 cgctggttga tgagattgtc ttcaaccacc aacacctggc acccggcaaa tagtgaagtt
      421 gagtcagccg attcaactga gtaagaggta ctggcgccaa cacccgccag ttgattttct
      481 ggaaacaggc tctcaaaaag ctgtcgcagg cgaattggtt tttgaaggta ccgaactgct
      541 ttgaattgat tgggaagctg gacgaactgg ctttgacccc agggagcaag caaaatcagg
      601 tttttctctg agattaactt gtgcggcaag gtttcgatca ggatggaaat atccaggcca
      661 ggctgagtga tatcaagaat gatggtgtca aacggggttg tggtcaattc tgcttcctgg
      721 attttgtcca gtaccgattc cgccgtggtt gcctggtcaa aggggagatg ccaggttgca
      781 agcatttggg taatcaattc aagacagggc gggtgatctt ccacaaccag tatgcgtcgg
      841 ttggaaaccg actggggaag ctcccacact ggtggtgtct gggcctgaac cgggaactca
      901 atttcaaacc agaaggtggt tccctggtcc atctggctct caaactgaac ttccccctgc
      961 atggcttcaa tcagtttttt ggaaatgacc aggccaagac cagtgccgcc aaagcgtcgg
     1021 gtgttgctgg aatccgcctg agtaaatggt tgaaacagga gtgactgttg ttctggtgaa
     1081 atcccgatcc cggtgtcctt gatttcaaat cgaaggcgaa cctgcccggt actctgattg
     1141 acgagtgaca cccgaagcgt cacctcacct tgatcggtaa atttaatgcc attgctgagc
     1201 aggttggtga gaacctgacg aagccgggct ggatctccac acagtatctg cggaacggtg
     1261 ggtgagacca cacaggtaaa ttccaactgt ttgcgctgag cagattcggc gagcaagtcc
     1321 atgacgccct ccaggactgg ctgcaattca aatggagact gttcgacctc aagttttcca
     1381 gcctcaattt tcgagaaatc cagaatgtca ttgataatca ggagcaaggc ttcgcctgac
     1441 cgataaatgg tttcggaata ttccttttgg
//
LOCUS       DAOQDO010001922         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101165_length_1469_cov_3.403112, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001922 DAOQDO010000000
VERSION     DAOQDO010001922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101165_length_1469_cov_3.403112"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..835
                     /locus_tag="PKZ53_23820"
     CDS             56..835
                     /locus_tag="PKZ53_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ43529.1"
                     /translation="MNVLTLTDGGVNFGQKWLVRGASLVLRPGSLTALVGPNGSGKTT
                     LLKCLAGLITPTEGTVVLDGKSVRGFPRRELARKITFVPQDTHLAFAFTVRDVVAMGR
                     HPHLRRFELETVADRQAIESALIRADVAHLSQRNVIKLSGGERQRVILARSLATEAPI
                     ILLDEPTASLDIAHALDVLELCRQLADEGKTVACALHDLNLVNRYATQVVMLSAGQVA
                     AVGTPAAVLTSANIESIFHVRAECVAASHSTQLFDFQRLPV"
     gene            847..>1469
                     /locus_tag="PKZ53_23825"
     CDS             847..>1469
                     /locus_tag="PKZ53_23825"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ43530.1"
                     /translation="MEKFGGLSAVGYVVGLLLVLGTVVGLIEGLKPPRIRPLQTATNT
                     LVPFPRDLQDGSGVCLEITRPPKRIVSQTLGTDEMLLDLCEPDRLAAVSVLASDPKYS
                     NVAGRTELAGKPVAKGPESILQLQPDLIFVASYSRAELVDLLRAAGAPVFRFSNFDRI
                     DDIKTNLRTLGYATGTDARAAHLVTQMETEIASIQARIPKTGTPPRV"
BASE COUNT          326 a          382 c          412 g          349 t
ORIGIN      
        1 accgctccgc caggtgaaac cgattttata aattataata aaattcggaa ctattatgaa
       61 tgtgctgaca ctaactgatg gtggagtcaa ttttggtcaa aaatggcttg tacggggtgc
      121 ttcgctggtt ttgcgaccag gttccttgac ggcactggtt gggccaaatg gttcaggaaa
      181 aacgacgttg ctcaaatgtc tggccggatt gatcactccc accgaaggaa ccgtggtcct
      241 ggatggaaaa tcggttcgtg gatttccccg gcgtgaactg gcccgaaaaa tcaccttcgt
      301 tccccaggac acccatctgg catttgcctt caccgtgcgc gacgtcgtcg ccatgggacg
      361 tcaccctcat ttgcgccgat ttgaacttga aaccgttgcc gaccggcagg ccatcgaatc
      421 agccttgatt cgagcggatg tggctcatct gagccagcga aatgtcatca aactttcagg
      481 tggggaacgg caacgggtta ttctggcgcg cagtctcgcc accgaagccc cgatcattct
      541 gctcgatgag ccgactgcca gtctggatat tgcccatgcg ctggatgtgc ttgaactctg
      601 tcgtcaactg gcggatgaag gaaaaaccgt ggcctgtgcc ctgcacgatc tgaatctggt
      661 caatcggtat gccactcagg tggtgatgct ttctgcgggt caggtggctg cggtgggaac
      721 gcccgccgca gtgttgacgt cagccaatat cgaatccatt tttcatgtgc gtgccgaatg
      781 cgtggcggcg tctcattcaa cacaactttt tgatttccaa cgacttcctg tctaagaaag
      841 caacatatgg aaaaatttgg agggctttca gccgtgggct atgtcgttgg gctgttgctt
      901 gtattgggga ctgtggtcgg gttgattgag ggactcaaac cacccaggat tcgcccatta
      961 caaaccgcga caaatacgct ggtgccgttt cctcgtgatc tgcaggatgg aagtggggtg
     1021 tgccttgaaa ttaccaggcc accaaaacgg atcgtgtctc agaccctcgg aaccgacgag
     1081 atgctgctgg atttgtgtga accagaccgc ctggcagcgg tgagtgtgct ggcttccgac
     1141 ccaaaataca gcaatgttgc tggccggaca gagctggctg gaaagccggt cgccaaaggg
     1201 ccggaatcca tcctccagct ccagccagat ctgatttttg tggcgagtta tagccgggca
     1261 gaactggtgg atttgctgcg ggcggcgggc gcgccggttt ttcgattctc aaattttgat
     1321 cgaattgacg acatcaaaac caacctgcga acgctcgggt acgccacggg gactgatgcc
     1381 agagccgccc atctcgttac gcagatggaa acagagattg ccagtattca ggctcgaatt
     1441 ccaaaaaccg gcaccccacc gcgtgtctt
//
LOCUS       DAOQDO010001923         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101173_length_1469_cov_3.188826, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001923 DAOQDO010000000
VERSION     DAOQDO010001923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101173_length_1469_cov_3.188826"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PKZ53_23830"
     CDS             complement(<1..1344)
                     /locus_tag="PKZ53_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNJ43531.1"
                     /translation="MKTRQRVALLFCLLISLTIIRSPYRIQAQTSTITINVDASANRR
                     PINPMIYGVAFASSQQLTDLNVPVHRSGGNNTSRYNWQQNADNRGFDWYFQSIAYSSS
                     TAGEVGDSFISSSKAAGAQPMLTIPMVGWVAKLGANRSKLCSYSIAKYGQQTGNDWQW
                     FPDAGNGILTNGQEITWNDPNDANTPANSTFQQGWVQHLVSRWGNAANGGLRYYILDN
                     EHSIWHSTHRDVHPTGATMDEVKSKMIDYGGKIKAIDPTAKVVGPEEWGWSGYLLSGY
                     DQQYGAQHGWSNLPDRANHGGMDYLPWLLDQLRQHQTSTGQRLLDVFTVHYYPQGGEF
                     SDDVSSGMQLRRNRSTRSLWDPTYTDETWINDKVQLVPRLRNWVNTYYPGTEIGITEY
                     NWGAEGHINGATTQADIFGIFGREGLDIGARWTTPATGTPTYNAIKMYRNYDGNKS"
BASE COUNT          331 a          362 c          381 g          395 t
ORIGIN      
        1 ggatttgttg ccgtcatagt tgcgatacat cttgatggca ttataggttg gggtgccggt
       61 tgctggtgtc gtccagcggg caccgatgtc gagcccttca cgtccaaaga tcccaaaaat
      121 atcagcctgg gtggtggcgc cgttgatgtg accttctgca ccccagttgt attcggtaat
      181 gccaatttcg gtgccggggt agtaggtatt gacccagttg cgaagccggg gaacaagctg
      241 gactttatca ttgatccagg tttcgtcagt gtaagtcgga tcccacaacg aacgggtcga
      301 gcgattgcgc cgaagctgca tacctgagga aacatcgtcg ctaaattcac cgccttgtgg
      361 gtaatagtga actgtaaaca catccagtaa ccgctgcccg gtgctggttt ggtgctggcg
      421 aagttggtca agcagccagg gaaggtaatc cattccgcca tgattggccc gatctggaag
      481 attgctccag ccgtgttggg cgccatactg ctggtcatat ccgctcagca gatacccgct
      541 ccagccccat tcttccggtc caactacttt cgcggttggg tcaatcgctt tgatcttccc
      601 accgtagtca atcatcttgg attttacctc atccatggtg gccccggttg gatgaacatc
      661 acgatgggtt gaatgccaga tgctgtgttc attatccaaa atgtaatagc gcagcccgcc
      721 gttggccgca tttccccagc gactgaccag atgctgaacc cacccttgct ggaaggttga
      781 attggctggg gtgttggcat cgttgggatc attccaggta atttcctggc cgttggtcag
      841 aattccattt ccggcatctg ggaaccattg ccagtcattt ccggtttgct ggccatattt
      901 ggcaattgaa taactacaga gttttgaccg atttgcccca agtttcgcca cccagccgac
      961 cattggaatt gtcagcattg gttgtgcccc agccgcttta ctcgatgaaa taaatgaatc
     1021 accgacttcg ccagcggtgc tgcttgagta ggcaatactc tgaaaatacc agtcaaatcc
     1081 gcgattatcc gcgttttgct gccagttata gcgactggtg ttattcccgc ccgaccgatg
     1141 taccgggaca ttcaaatcag tgagttgttg cgatgaggca aacgccactc cataaatcat
     1201 cgggttgatt ggacgacgat tggcgctagc gtcaacgttg attgtaatgg ttgaagtctg
     1261 ggcttgtatt cggtatggac tccgaatgat ggtcagactt attaaaaggc aaaacagcag
     1321 ggccactctc tgacgagttt tcatgggggt ctcctccgcc tgcccaaact ctgaaatttt
     1381 tgaatttgat tgcaatccgt ggtacttcgt aagtaaatga aaataaatta cttgcgaaga
     1441 gaatttgtct ggccggcagt tcaaaaata
//
LOCUS       DAOQDO010001924         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101419_length_1467_cov_3.601983, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001924 DAOQDO010000000
VERSION     DAOQDO010001924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101419_length_1467_cov_3.601983"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKZ53_23835"
     CDS             <1..393
                     /locus_tag="PKZ53_23835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43532.1"
                     /translation="IAAREKMSAKVRADAKAETPDAKARIPAKEKVDAPLMAQPPQIL
                     LPNIAVKAKMNAKVRADARPETPDAKARIRAKERADAPPMPRLRLKRKMTKPLPLRKK
                     TPKPAKIKIVVAEKMVAVPSSNLFLEPT"
     gene            712..>1467
                     /locus_tag="PKZ53_23840"
     CDS             712..>1467
                     /locus_tag="PKZ53_23840"
                     /inference="COORDINATES: protein motif:HMM:NF026947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HNJ43533.1"
                     /translation="MTIQVYHLERLNNPARKIAFSFHFLAKLGTVSASDLYDCRLVLR
                     HFIEQSLDEKPLCGTPLIVGLAESGILLSALLHQEAHQLGITTGWICSTRRPATGIQF
                     TERHSHQPAHILPVPASPVDELWLIEDEITTGQTIFQVIRHLSSLTSVKMVRIFTLVD
                     ARSDEQRTAFSELLNRDGIQPLVHSLLEFHGFPTMECFDRKFLEESTLSPSLCTQESV
                     DHWMLPVARPTLSVQTAPTLDAFDGLEGSLLVIG"
BASE COUNT          373 a          366 c          360 g          368 t
ORIGIN      
        1 atagctgcaa gggaaaaaat gagtgcaaag gtcagggcgg atgcaaaggc ggagacgccg
       61 gatgcaaagg caagaattcc tgcaaaggaa aaggtggatg cgccactgat ggctcagccg
      121 ccccagattc tgctgccaaa catagctgta aaggcaaaaa tgaatgcaaa ggtcagggcg
      181 gatgcaagac cggagacgcc ggatgcaaag gcaagaattc gtgcaaagga aagggcggat
      241 gcgccaccga tgccgcggct ccggctgaaa agaaagatga caaagccgct gcccctgaga
      301 aaaaagacac caaagccagc aaagataaaa atagttgtgg cggaaaagat ggttgcggtt
      361 ccaagcagta atctttttct cgaaccaacc taattgactg cggaacatct gagcccacgc
      421 tttcagcaca ctttattttc tgaaaggtga tcacctgttc cgcagttttt cgttcactga
      481 tcgcgtttat ttaaacttct gttgaatcta attttgatgg tgttaacaag ctggccctat
      541 tttccacctg ctgattgtcg gtcctgaacg acgtataccg cttttcactg cagcgcttga
      601 attagaaagc aggtaagtcg ttcaatcaaa taagcttagt ggacgactga cgactgacaa
      661 ctggcgacaa actggtatta tttattttcc gaaggaatta ttttatacgc tatgacaatt
      721 caggtttatc atcttgagcg gctcaataat ccagcccgaa aaatcgcgtt tagttttcat
      781 ttcctggcca aactgggaac ggtttcagct tctgatttat acgactgccg tctggtgctg
      841 cgtcatttta ttgaacaatc gttggatgag aaacctctgt gcggtacccc tctgattgtg
      901 gggctggctg aatcgggcat tttgctttca gcgttactac atcaggaagc gcatcagttg
      961 gggattacga ctggatggat ttgctctacc cgacggcctg ccaccggcat ccagtttacc
     1021 gagcgccact ctcatcaacc cgcgcatatc ctccccgtgc cagccagccc cgtggacgaa
     1081 ctctggttga tcgaagacga aatcaccact ggccagacca tctttcaggt cattcgccac
     1141 ctgtcatcgc tgacctcggt caagatggtt cgtattttca ccctggttga tgcccgaagc
     1201 gacgaacaac ggaccgcttt ttcggaattg ctgaaccgtg atggcatcca gccgttggtt
     1261 cattcactgc tggagtttca tggctttcca accatggaat gttttgaccg gaaatttctt
     1321 gaagaatcaa ctctgtcacc cagtttgtgt acccaggaat cagttgatca ctggatgcta
     1381 ccggtggctc gcccgacctt gtccgttcaa accgcaccaa ccctcgatgc ctttgacggt
     1441 cttgaagggt cactcctggt cattggc
//
LOCUS       DAOQDO010001925         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101458_length_1467_cov_2.614023, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001925 DAOQDO010000000
VERSION     DAOQDO010001925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101458_length_1467_cov_2.614023"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="PKZ53_23845"
     CDS             complement(<1..1412)
                     /locus_tag="PKZ53_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43534.1"
                     /translation="MQLFLKALIIIVLGIGVFFTWYEVTTLTPVEIHTQVVASAARLA
                     IAPQTEFSSRLDTFRKHLAEADPDTSRYPKTWAALSALVEKRDKLQTEKNARTPATDF
                     PVPAVEPLLAACEQEAVTMQTQAKSAMQHRLRLMSGCFWSLVTIFGLGLWIYQQQLIR
                     QLGKYSLSRPRSLSEAISNLIDNFESLQRDMAVRERQALDVALMQRRIKMHSTGSMEP
                     VKPIPVGPSPATIDSGKASPSRQSTGKIEPVKPMAGGPEKAITPPSPVALPEPYFPLS
                     HSQSSEKFPSTSSSGSVPAFTLPSEESASLPATRPPTQELFADPAPVKYARLADSIGG
                     FILGDTTDPTLVPRIPIGSGQFSQFEYLQRQNGTLVAIALQDVELFQHIYIKPEVVLE
                     AALQNIVNLLLRCRVRPSEIAIEHDTVFWHIPPEVDISPTDIESFLLNAYRDSVFVFG
                     DRQLELPEISITIPPRGSFFG"
BASE COUNT          355 a          362 c          398 g          352 t
ORIGIN      
        1 ccgaaaaatg agccacgcgg cgggatggtg attgaaattt caggtaattc caattgccgg
       61 tctccgaaga caaacacgct gtctcgatag gcattgagca agaatgattc gatgtcagtt
      121 ggtgaaatat caacttcggg tggaatgtgc caaaacaccg tgtcgtgctc aatggcgatt
      181 tcagatggtc gaactcgaca ccgaagcaac aagttgacaa tgttttggag cgctgcttct
      241 aagaccactt ctggtttgat gtaaatatgt tgaaagagtt caacatcctg taacgcaatc
      301 gccaccagtg ttccgttttg tcgctgaagg tattcaaact gactgaactg acctgaacca
      361 attggaatcc gcggcaccaa cgtcgggtca gtggtatcgc ctaaaataaa gcccccgatg
      421 ctatcagcca gccgcgcata cttgacgggt gccgggtcag caaaaagctc ctgggttggc
      481 ggtctggtcg caggcagcga cgcactttct tcagatggta gtgtaaaggc aggcaccgag
      541 ccgctactgg aggttgaggg aaatttttct gacgattgac tatgagacaa cgggaagtag
      601 ggctcaggta acgcgaccgg cgacggagga gtaatcgcct tttcaggtcc cccagccatt
      661 ggtttgaccg gttctatttt tccggttgac tgcctggaag gtgacgcttt cccagaatct
      721 attgtggccg gcgatggtcc gactgggatg ggtttgactg gctccatact cccggtggaa
      781 tgcattttga tccggcgctg catcaaggcc acgtccagcg cctgtcgttc gcgaactgcc
      841 atgtcgcgct gtaaactctc gaaattgtca atcaagttgg agatggcttc agacaatgag
      901 cgcgggcggg ataaactata ttttccaagc tgccggatca gttgttgctg atagatccac
      961 aatcccagcc caaaaatggt cacaaggctc caaaaacagc cactcatcag ccgtaaccgg
     1021 tgctgcatgg ctgattttgc ctgtgtttgc atcgtaaccg cctcttgttc acaggcagcc
     1081 agtaatggtt caaccgccgg cacggggaag tcagtggccg gagtcctggc gtttttttcc
     1141 gtttgcaatt tgtcacgttt ttccactaac gctgacaatg ccgcccaggt ttttgggtac
     1201 cgtgaagtgt cagggtcagc ctcagctaaa tgctttcgga acgtgtctaa cctggaggag
     1261 aactcagtct gcggagcgat tgccagccgg gcagccgaag caaccacctg agtatggatt
     1321 tcaactggtg tcagggttgt gacctcgtac caggtgaaga atactccgat tcccaacaca
     1381 ataatgatga gagctttcag aaataactgc acaggcgcag ggctccttca gacggttaag
     1441 cacaaacgga gctacatctt ggcagga
//
LOCUS       DAOQDO010001926         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101479_length_1467_cov_2.310907, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001926 DAOQDO010000000
VERSION     DAOQDO010001926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101479_length_1467_cov_2.310907"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKZ53_23850"
     CDS             <1..255
                     /locus_tag="PKZ53_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HNJ43535.1"
                     /translation="SLSSPTAIPANGALPFGRQEALTQLRRLAEFFRATEPHSPVSYL
                     VQRAVKWGEASLEDVLGELIKNEEVLDHVRETLGIQGAQS"
     gene            314..883
                     /gene="tssB"
                     /locus_tag="PKZ53_23855"
     CDS             314..883
                     /gene="tssB"
                     /locus_tag="PKZ53_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019333264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNJ43536.1"
                     /translation="MPKKESLQEKLSRVRPPRVHIKYEVEIGGAIELKEIPFVVGVIG
                     DFSGQPAEPLKPLRDRNFTYIDRDNFNDILKRMTPRLALRVANRLNRKDEKLNVELKF
                     NRIEDFEPDQLAQQVPALKKLIEARNKLSGLLTKMDGNNQLESVLQSVLSDTEKRNQL
                     GRDLGIEPPRTSPSQPGQSLEFPEEEPAQ"
     gene            880..>1467
                     /locus_tag="PKZ53_23860"
     CDS             880..>1467
                     /locus_tag="PKZ53_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNJ43537.1"
                     /translation="MSTQSNPELKMVTAPQPGGGSLLDQILAEGRLAHSPEQRPHAIN
                     LISELVDQVLQGQLVMSNDMEAAINERIKQIDALLSDQLNEIMHHPDFQKLEASWRGL
                     HHLVFETETSTMLKIRVLNATKRELIKDFERGGNDPQSGQGNEFDQSSVFKKVYEAEY
                     GMFGGEPYGMLLGDLEFTNHPQDMYLLEKMSGVAAA"
BASE COUNT          397 a          390 c          360 g          320 t
ORIGIN      
        1 tctctgagtt ctccgaccgc gataccagcg aatggggcac tcccatttgg gcgccaggaa
       61 gcactcaccc agttacgccg actggccgaa tttttccgcg ccaccgaacc tcacagcccg
      121 gtttcctatc tggtgcaacg ggcagtgaaa tggggtgagg cttcactcga agacgttctg
      181 ggcgaactga ttaaaaacga ggaggttctt gatcatgtcc gcgaaacctt agggattcag
      241 ggagcccaat cctgaactca atctggtttc ttcatcactc atcacacatc tcaatttacg
      301 aggtatacct ttcatgccga aaaaagaaag tttgcaggaa aaactcagcc gtgttcgccc
      361 accccgagtt catatcaagt atgaagtgga aatcggcggt gctattgaac tcaaagaaat
      421 tccatttgtg gtgggtgtga tcggggattt ctccggacag cccgccgaac ccttaaagcc
      481 cctccgggac cgaaatttta cctatattga tcgcgacaat tttaatgaca tcctcaaacg
      541 aatgacgccc cggcttgcac tgcgagtggc aaaccggctc aatcgcaaag atgaaaagtt
      601 gaacgttgag ctgaagttca accggattga ggacttcgaa cccgaccagc ttgcccaaca
      661 ggtgccggcg ctcaagaaac tgattgaagc cagaaacaag ctctcgggtc tgctcaccaa
      721 aatggacggg aacaaccagt tggaatccgt gttgcaatcg gtgttgtccg ataccgaaaa
      781 acgaaaccag cttggccgtg atctgggtat cgaaccaccc cgaaccagtc catcacaacc
      841 tggtcaaagt ctcgaattcc ctgaggagga gccagcacaa tgagcacaca atccaacccc
      901 gaactcaaaa tggtgacggc gcctcaaccc ggaggtggat ctttgttgga tcaaatcctg
      961 gctgaaggcc gacttgccca ctcacccgaa caacgcccac acgccatcaa tttgatttct
     1021 gaactggtgg accaggtcct gcagggacaa ctggtgatgt caaacgacat ggaagccgcc
     1081 atcaatgaac gcattaagca gattgatgcc ctgctctcgg accagctcaa tgaaattatg
     1141 catcaccctg attttcaaaa gctggaggcc tcgtggcgcg gactccacca tctggtgttt
     1201 gaaactgaaa ccagcaccat gctgaaaatc agagttctca atgccaccaa gcgtgagttg
     1261 atcaaagatt ttgaacgggg tggcaatgat ccacaaagcg gccaggggaa cgaatttgat
     1321 caatcctcgg tctttaaaaa ggtttatgaa gccgaatacg gcatgtttgg tggtgaaccc
     1381 tatggcatgc tgttgggtga tcttgagttt accaaccacc cccaggatat gtatctgctg
     1441 gaaaaaatgt caggtgtcgc cgccgcg
//
LOCUS       DAOQDO010001927         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101601_length_1466_cov_2.518072, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001927 DAOQDO010000000
VERSION     DAOQDO010001927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101601_length_1466_cov_2.518072"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKZ53_23865"
     CDS             complement(<1..471)
                     /locus_tag="PKZ53_23865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ43538.1"
                     /translation="MGDLKEPPQGYGDGMSLSSSTVIDSLQSESNTLPTQHQLNQTST
                     SIRTTDLIGQMLGDKYRIHRLLGQGGAGAVYHATQLDLDRPVAIKVLRSDVVCDVVAI
                     ERFRREARAAARLHHPNVVAVYDFGMLPEICAFIAMEYLTGRTLREEIRKFPTGL"
     gene            complement(747..>1466)
                     /locus_tag="PKZ53_23870"
     CDS             complement(747..>1466)
                     /locus_tag="PKZ53_23870"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ43539.1"
                     /translation="SFYKYLHLAGLVPINPADTRLVTPPPPPEMATTNALSPKEVRML
                     LGSPDRKEVTGARDYVVLLLLARLALRAAEVCSLRNQDIVRVSARDVRGRSINWVIRV
                     RVKGGRERKLPLPDDVKTAIEDYLKLDDEMRRLTKGLNPDAALIQRTGMGRTLGGKPL
                     TTRALWNIVNKYAQYVGLEQVHPHSFRHTAITRALDMGLSYRDVQMMTGHKTLLTVQR
                     YDAHREDMSRNAINELNYEEE"
BASE COUNT          353 a          416 c          360 g          337 t
ORIGIN      
        1 tagaccagtt gggaactttc gaatttcttc gcgcagtgtc cggccagtca gatactccat
       61 ggcgataaag gcacaaattt ccggaagcat gccaaagtca tataccgcaa ccacattggg
      121 atgatggagc ctggccgccg cccgcgcttc acggcgaaag cgttcaatcg ccaccacatc
      181 acacaccaca tcggaccgta aaactttgat tgccactgga cggtccaggt caagctgggt
      241 ggcatgatag accgcaccag ccccaccttg ccccagcaac cgatggatcc ggtacttgtc
      301 gcctaacatc tgcccaatta aatctgtagt tcggatgctg gtgctcgtct ggtttaactg
      361 atgttgtgtc ggaagagtgt tgctttcgga ttgcaatgag tcaatcacag ttgaactgct
      421 caacgacatt ccgtccccat acccctgagg tggttctttc agatctccca taaattccct
      481 ttgctcaaaa cccttccggt gatggctacc tggttgacga accagggtta acaatgggtc
      541 cacgtaacga ttggaaattt gggtcacgcg cctcttgctg gcacaaccgg gattccatag
      601 gggctggcgg caaaagagtg gactcaccca aacaagcaat tgaatcaaga agcccccatc
      661 atactccaga cccaggtcac ccgaaaacct gaatccacgc ccccgcagac agtcataatc
      721 aattcaattc aaatcacact tcatttctat tcctcttcat aatttaattc attaatggca
      781 ttgcgcgaca tatcttcccg gtgggcgtca tagcgttgaa cggtgagcag ggttttatgc
      841 ccggtcatca tctggacatc acggtaactc agccccatgt cgagcgccct ggtaatcgca
      901 gtgtggcgaa atgaatgtgg gtgaacctgt tcaaggccaa catattgggc atatttattg
      961 acgatgttcc acaaggcacg ggtggtcagg ggttttccac ctaacgtccg gcccattccc
     1021 gtccgctgga tcaaagcagc atcgggattg agacctttgg tgaggcgtcg catctcatca
     1081 tccagcttta agtagtcttc aatcgcagtt tttacatcat ctgggagcgg gagttttcgc
     1141 tcacgtccac ctttgacccg gacgcgaata acccagttga tcgaacgtcc ccgaacgtca
     1201 cgggccgaca cccgcacaat gtcctgattg cgaaggctac agacctcagc cgcccgcagt
     1261 gccagcctcg ccagcaagag gagcaccaca taatcacggg caccagtgac ctctttacgg
     1321 tccgggctgc caagcaacat ccggacttct ttgggggaca gagcattggt ggtggccatt
     1381 tcaggtggcg gtggcggggt caccagccgg gtatcagccg ggttgatcgg caccaatcca
     1441 gccagatgca gatatttata aaaact
//
LOCUS       DAOQDO010001928         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_101987_length_1463_cov_2.769176, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001928 DAOQDO010000000
VERSION     DAOQDO010001928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101987_length_1463_cov_2.769176"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PKZ53_23875"
     CDS             <1..1119
                     /locus_tag="PKZ53_23875"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ43540.1"
                     /translation="SSFFILHSSFFILHSSFKMSGPAVNIVTGFLGSGKTTLLQHVLK
                     HGLNGRRVALIINEMADLGIDGQIVECANVERMIELNNGCICCTINREFASAIQSIIE
                     TANPHLIIIETTGAAEVMPLIDEVYTCGLRVDAVITVVDALNFFQAAETSDVTMEQVA
                     GADFLVLNKLDLVTARPLGKVRRALRKLNPRALILETVRGALESDVLFATSAASFREH
                     LRQSPSPAQSAIVATTDTPPVLERYLATATGNQPSSRGNSRSLPVAVSNHLQESDLSA
                     FSYTSSKPLKRLAFEQFLEGLPPAIYRAKGIVHFDGERWPCLFNFTCGRYDLDPLLYP
                     VTATFQNQAIFIGHTISTVRHQILPRLQACEQNSPNHE"
     gene            1217..>1463
                     /locus_tag="PKZ53_23880"
     CDS             1217..>1463
                     /locus_tag="PKZ53_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43541.1"
                     /translation="MEQEVLERLHQKLLDKRRELQIALNIESQKDSQMDEEGKDPLDA
                     AATYTNRELVARQIDQYRQLLLDVEEALERVEDGTYGI"
BASE COUNT          373 a          391 c          328 g          371 t
ORIGIN      
        1 tcttcattct tcattcttca ttcttcattc ttcattcttc attcttcatt caaaatgtct
       61 ggaccagccg ttaacattgt cactggattt ttaggcagcg gaaaaaccac gctgctccaa
      121 cacgtgctca agcacggtct caatggccgt cgggtcgcct tgattatcaa cgaaatggcg
      181 gacctgggaa ttgatggtca gattgtcgaa tgcgccaatg tcgagcgcat gattgaactt
      241 aacaacggat gtatctgctg caccatcaac cgcgagtttg catcagcgat tcaatccatt
      301 attgaaaccg ccaatccaca cctgatcatt attgaaacca ctggcgctgc cgaagtcatg
      361 ccactcattg atgaggttta cacctgcggg ttacgggtgg acgccgtcat caccgtggtg
      421 gacgcactca acttttttca ggcggctgag accagcgatg tcaccatgga acaggtcgcc
      481 ggggccgatt ttctggtcct caacaagctc gatctggtta cggcccggcc tttgggaaaa
      541 gtgcgacgag cgctccgcaa actcaatcca cgggcgttga ttttggaaac ggttcgcggc
      601 gcgctggaaa gcgatgtgct gtttgcgacc agtgcggcct cgtttcgcga acatctccgc
      661 cagtcaccct caccggctca atcagcgatt gtcgccacca ctgacacccc acctgtttta
      721 gagcgctatc tggcgacagc aactgggaat cagccttcca gtcgtggcaa ttcacgtagc
      781 cttccagtgg ccgtcagcaa tcatctgcag gaatctgacc tttcggcctt tagctatacc
      841 agttccaaac cgttgaagcg actggccttt gaacaatttc tcgaaggatt gccaccagct
      901 atctaccggg caaaaggaat tgttcatttt gatggcgaac gctggccctg cctgtttaat
      961 ttcacctgtg gccgctatga tctggacccg ttactctatc cagtgacagc aacgtttcaa
     1021 aatcaggcaa tttttattgg ccataccatc tcaactgtca gacaccaaat cttacccaga
     1081 cttcaggcgt gtgaacagaa ttctccaaat cacgaataaa ctatccactt tttgcaatcc
     1141 atcgttgaca aagcaagagt tggtttttta ttttggcaaa ccttaatcta tccttaaaga
     1201 aaaacggtaa ctcctcatgg aacaagaagt tcttgaacgg ctgcatcaaa aattgctcga
     1261 caaacggaga gaactccaaa ttgccctgaa cattgagtcc caaaaagact ctcaaatgga
     1321 tgaagaaggc aaagacccgc tcgatgcagc agcgacttat accaatcgcg aactggttgc
     1381 tcgccagatt gatcagtatc gtcaactcct gctggatgtt gaagaagcgt tggaacgagt
     1441 cgaagatgga acctatggca tct
//
LOCUS       DAOQDO010001929         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102245_length_1461_cov_4.049787, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001929 DAOQDO010000000
VERSION     DAOQDO010001929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102245_length_1461_cov_4.049787"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKZ53_23885"
     CDS             complement(<1..284)
                     /locus_tag="PKZ53_23885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ43542.1"
                     /translation="MKKLGRGNFGVVWLAEHRTSVVTTQVALKLPIEEDIDVEAVKRE
                     AAIWVKASGHPNILPIISADVFQGQIVIVSEYAPGGSLYDWLERYGGKAPS"
     gene            867..>1461
                     /locus_tag="PKZ53_23890"
     CDS             867..>1461
                     /locus_tag="PKZ53_23890"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNJ43543.1"
                     /translation="MFRSFVSSNTSLFTGAATVAQSLGLFLPKATASLWIHGTSIEAF
                     QSARSLIFRLQHQIRPCRWAFTGIDFDTLTWLQKRYIDDFVYLPPVSVSLSINRFFQS
                     VRPQALVILGSGEGLSKAVFSRACQQNIPIIWTEIDETVIPQLQQLWKAGDLHPANSR
                     FGVQTSLVKEQLQASGIPAPFISVTGSLQYEFPPEPSQ"
BASE COUNT          365 a          373 c          315 g          408 t
ORIGIN      
        1 gaaggcgctt ttccgccgta ccgttcaagc cagtcataga gcgacccgcc cggagcatat
       61 tcgctgacaa tcacaatctg tccttgaaac acatccgcgc tgatgattgg caaaatattc
      121 ggatgacccg atgccttcac ccaaatggcg gcttcacgtt tgacagcttc cacatcaatg
      181 tcttcttcaa tcggaagctt gagcgccacc tgagtggtga caaccgatgt ccgatgttca
      241 gccagccaga ccacaccaaa attgccacgg ccaagctttt tcacaaggat gtaaggtcca
      301 atctgagcac cagatgacag cataatagac attatcggag ttagggtttt gcctcattct
      361 gactattgat ttaaaagcac ttacaatcgt gatttcctta tcaccgataa tgtctaatat
      421 ctttcagggt tcagggttca gggttcaggg ttcaaaattg taagggaaga caattttatc
      481 aataataagt accttgtcat aagttttctg agatattggg tttggtaagt gcttgattct
      541 tttccgtctt ttccgtgttt tccgtggtta aaatgtttgg aaattttcgg taaggtactt
      601 accatttagg aaggaatcat ctggatcagc aaatagttaa ctcattgaaa gagttgtatt
      661 cctctgaatc gtgaatcctt agacgaaaga gcacatcatt gtgccgcaac tgagtttaaa
      721 ctcaaatagt cctgtcaatg gcaactgaaa aaacaggttg caggattgga tttttcttcc
      781 taaagtggca gcccccgtca cgtttctggt tcatccaaat accccaaatg ctgaacatgc
      841 ccattttccc gccgcaggaa agcactatgt tccgctcgtt tgtcagttca aatacttcac
      901 tcttcactgg tgccgcaacc gttgcccaaa gtttgggcct gtttttgcca aaagccacgg
      961 cctcactctg gatccacggc acatcaatcg aagcctttca atcggcgcgc tccctcattt
     1021 ttcgcctgca acaccagatc cgcccctgtc ggtgggcgtt taccgggatt gactttgaca
     1081 ccttaacctg gctgcagaaa cgctacattg atgatttcgt gtatctgcca ccagtttcgg
     1141 tctcgctgag catcaatcgt ttctttcaat cggttcggcc acaggcattg gtgattctcg
     1201 gctcgggtga aggattgtca aaagctgttt tttcaagggc ttgccagcaa aatattccca
     1261 tcatctggac tgaaattgat gaaacagtaa tcccacagct tcagcagttg tggaaagccg
     1321 gggatcttca ccctgcgaat tccaggtttg gcgtccaaac atccctggtg aaagagcaac
     1381 ttcaggcatc gggcatcccc gccccgttca tttcggtcac cggcagcctg cagtatgaat
     1441 ttcccccaga accgtctcaa c
//
LOCUS       DAOQDO010001930         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102383_length_1460_cov_3.545907, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001930 DAOQDO010000000
VERSION     DAOQDO010001930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102383_length_1460_cov_3.545907"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PKZ53_23895"
     CDS             complement(<1..156)
                     /locus_tag="PKZ53_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ43544.1"
                     /translation="MLPNLVIIGAMKCGTTSLHGYLSLHPQIFMSEQKELNFFIKSKG
                     WANGLDWY"
     gene            322..927
                     /locus_tag="PKZ53_23900"
     CDS             322..927
                     /locus_tag="PKZ53_23900"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNJ43545.1"
                     /translation="MHIEHHPRHSGWIEVIAGGMFSGKTEALIHRLRRAQIARLRVQT
                     FKHEIDERYATYHIVSHSQLRFEAENVTRSLDILPLVKAETDVVGIDEGHFFDTNLLE
                     VCSELANQGKRVIVAGLDQDYLGRPFEPMPQLLAIAEYITKLQAICAQCGNPANFSQR
                     LVANEARVLVGADGFYEARCRRCFVPQVLRVETAVKAESEH"
     gene            932..1411
                     /gene="ispF"
                     /locus_tag="PKZ53_23905"
     CDS             932..1411
                     /gene="ispF"
                     /locus_tag="PKZ53_23905"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNJ43546.1"
                     /translation="MFRIGIGYDVHRLVDGRKLIIGGVEIPYDRGLHGHSDADVLTHA
                     ICDALLGAAALGDIGQHFSDRDPQWAGIDSFHMLKHVVHLVTQRQMQVVNVDSNVVAQ
                     APKLAPYIPAIRQRLADALNLPLDAVSVKAKTNEGLDSIGRSEGIAAQAAVLVKTQD"
BASE COUNT          345 a          376 c          403 g          336 t
ORIGIN      
        1 ataccagtcc aacccgtttg cccagccttt agacttgata aagaaattta gctctttttg
       61 ctcagacata aaaatctgag gatgcaggct caggtagccg tggaggctgg tggtcccaca
      121 tttcatggca ccaatgatta caaggttggg aagcatagat ttttgatttt cttcagagat
      181 gtgataagag agaatgttcg aaactgccac aaataacaca caaaggattt tcgggcaatc
      241 tttccaccgg gaaagtcaga gagggactct ctctgtggtt ggctctcgtc ggttggtcgt
      301 ggcccgaagc acaaaccacc tatgcacatt gaacatcacc cacgacactc cggctggatc
      361 gaagtcattg ccggaggcat gttttctgga aaaaccgaag ccctgattca ccggctccgc
      421 cgcgcccaaa ttgcccgatt aagggtccag accttcaagc atgaaattga cgagcgctat
      481 gcgacctatc atatcgtctc gcacagccag cttcgattcg aggctgaaaa tgtgacgcga
      541 tccctggaca ttctcccgct ggtcaaagct gaaactgatg tggttgggat tgatgaaggc
      601 cacttctttg acaccaattt gcttgaagtg tgctctgaac ttgccaacca gggaaagcga
      661 gtgatcgtgg ctggcctgga tcaggattac ctgggacgtc cctttgaacc aatgccacaa
      721 ttgctggcga ttgccgagta catcaccaag ctccaggcga tttgtgccca atgcggcaat
      781 cccgctaatt tctcgcagcg gctggtcgcc aacgaagccc gggtcctggt cggggccgat
      841 ggattttatg aagcccggtg tcgccggtgt tttgtgccgc aggttctgag agttgaaacg
      901 gctgtcaaag ctgaaagtga gcattgaacc catgtttcga atcggaatcg gatatgacgt
      961 ccatcgtctg gtggacgggc gcaagttgat catcggcgga gttgaaatcc cgtatgatcg
     1021 tgggttgcac ggtcattcgg atgccgatgt cctgacccat gccatttgcg atgcactgct
     1081 tggggccgcc gcattgggcg acatcgggca gcacttctct gatcgtgatc ctcaatgggc
     1141 aggcattgat agttttcaca tgctcaaaca cgtcgtgcat ctggtcactc agcgccagat
     1201 gcaggtggtc aatgttgaca gtaatgtggt tgcccaggcg ccaaaactgg cgccctatat
     1261 tcccgccatc cgtcagcgac tggctgatgc gctcaacctg ccactcgacg ccgtgagtgt
     1321 gaaagccaaa accaacgaag ggctggactc aatcggacgc agcgaaggca ttgcggccca
     1381 ggcagccgtt cttgtgaaga cccaggactg aagaaggcgg gctgaaaaaa ccagggcctg
     1441 gggctgaggg ctgaagattc
//
LOCUS       DAOQDO010001931         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102384_length_1460_cov_3.531673, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001931 DAOQDO010000000
VERSION     DAOQDO010001931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102384_length_1460_cov_3.531673"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKZ53_23910"
     CDS             <1..192
                     /locus_tag="PKZ53_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43547.1"
                     /translation="FCFQQIPDVSSVWGSFGVAFAWEKDGFASSGFPSVLSELLFLHP
                     DLFISTASEIIFKKKETIP"
     gene            189..>1460
                     /locus_tag="PKZ53_23915"
     CDS             189..>1460
                     /locus_tag="PKZ53_23915"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNJ43548.1"
                     /translation="MKNLWQTIRGKVYLSLMVALAVFGSGWAEVAQVHAQQGDPPPLI
                     KKGSVEFTGSLRTRYENWGWFDVPGRDSQQDFVGTLFRFALKGKHSLNDTHSIEWAAE
                     AAFPSLIGLPRNAVAPAPQGALGLGGTYAAGSGDERASIFLKQAYLRWGIKGQKFNTG
                     IKIGRFEFSEGAELAATDPTIGALKRTRIQERLIGPFGFTHVGRSLDGGQLSFGTSTT
                     NVTVLAARPTEGVFQLDGMRQIPGVSVVYASFNKALHAHKVNDKTQHQGEARVFGLYY
                     RDTRSVLKTDNRSIIAREADRQNDINVTTIGGHYVRDFKLGKGKADVLLWGAGQFGSW
                     GALDQRAGSIVAEVGYQPEMKWNPWIRAGFTHSTGDDDPTDNEHGTFFQVLPTPRIYA
                     RTPFFNMMNNQDIFAEVIVRPTKKLNIRSDFH"
BASE COUNT          348 a          343 c          393 g          376 t
ORIGIN      
        1 ttttgttttc aacaaatacc agatgtttcc agtgtatggg gtagtttcgg cgtagcgttt
       61 gcatgggaaa aagatgggtt cgcctcaagt ggttttccct cagtcctgag tgagctgctt
      121 tttcttcatc cggacctttt catatccaca gcttcagaaa tcatcttcaa aaagaaggaa
      181 acaattccat gaaaaatctc tggcaaacga ttcgcggtaa ggtttatctc tccttgatgg
      241 tcgctctggc agtgttcggc agtggttggg ctgaagtggc acaagttcat gctcaacaag
      301 gtgatccacc acctttgata aaaaaaggtt cagttgaatt cactggcagt ttgcggacac
      361 ggtatgaaaa ctggggctgg tttgatgttc cgggtcgtga tagtcagcag gattttgtcg
      421 ggacgctctt tcgatttgcc ctcaagggaa aacattccct caatgatacc cattctattg
      481 agtgggcagc cgaggcggcg tttccgtcat tgattggctt gccgcgcaat gcggtggcgc
      541 cagctccgca aggcgccctt ggcctgggtg gaacgtatgc tgccgggagt ggtgacgaac
      601 gggccagtat tttcctcaag caggcatatt tacggtgggg tatcaaaggc cagaagttca
      661 atactggcat taagatcggt cgttttgaat ttagcgaagg ggctgaacta gcggcgactg
      721 atccaaccat cggagcattg aaaaggactc ggattcagga acggttgatt gggccctttg
      781 gatttaccca tgttggtcgc agccttgacg gtgggcagct ttcctttggc acttcgacga
      841 ctaatgtcac ggtgctggcc gctcgaccaa ccgaaggggt gtttcagctt gatggcatgc
      901 gtcagattcc tggcgtaagt gtcgtgtatg cgtcgttcaa caaagcgctg catgcccaca
      961 aagtcaacga caaaacccag catcagggcg aagctcgggt gtttggcctg tattatcgag
     1021 atactcgatc cgtgctcaaa accgacaatc gttcaattat agcccgcgaa gccgaccgcc
     1081 aaaatgatat caatgtgaca accatcggcg ggcactatgt tcgtgacttc aaactgggca
     1141 aaggaaaagc ggatgtgctc ctgtggggcg ccgggcaatt tggaagttgg ggagcccttg
     1201 atcagcgggc aggttccatt gtggctgaag tcggttatca acctgaaatg aagtggaatc
     1261 cgtggatccg ggctggtttt acccacagca ccggcgacga tgatcccacg gacaacgaac
     1321 acggtacatt tttccaggtg ctgccaacac cacgcattta tgcccgaact ccattcttta
     1381 atatgatgaa caaccaggat atttttgctg aagtgattgt tcgtcccacc aagaaactca
     1441 atattcgctc agatttccat
//
LOCUS       DAOQDO010001932         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102421_length_1460_cov_2.498221, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001932 DAOQDO010000000
VERSION     DAOQDO010001932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102421_length_1460_cov_2.498221"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PKZ53_23920"
     CDS             complement(<1..1291)
                     /locus_tag="PKZ53_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNJ43549.1"
                     /translation="MSERKILTDESGAPVVDNQHSQTAGPNGPVLLQDHHLVEKLARF
                     NREKIPERIVHARGSAAYGYFEVTHDVTQWTKAKFLSEIGKRTEVFLRFSTVALELGS
                     ADTVRDPRGFAVKFYTEEGNYDLVGNNTPIFFIRDPQKFPDFIHSQKRDPYTHVQDPN
                     NAWDFFSHSPELTHQFTWLYGDRGIPYSYRHMDGFGSHTFQWLNAQGEAFWVKYHFKT
                     DQGIKCLPSAEAARIAGENPNFHHLDLLHAIDRGDFPSWTLKMQIMPVADAAHYRFNP
                     FDLTKVWPYADYPLIEVGKLVLNRNPDNYFADVEQSAFNPANFVPGIGPSPDKMLQGR
                     LFAYGDAHRYRLGVNHTHLPVNRPHATEAHNYGRDGAMRFDGNGGRSKNYEPNSHGGP
                     VQSNEPHYAGIPVQGVTGSHSWEHHAEGDDFVQAGSLY"
BASE COUNT          338 a          344 c          426 g          352 t
ORIGIN      
        1 ggtagagact cccggcctga acgaagtcat caccttcggc atgatgttcc catgagtgtg
       61 agccagtcac accttgaact ggaatcccgg catagtgcgg ctcgtttgat tggacaggcc
      121 cgccgtggct gttgggttca taattttttg accggccacc attgccgtca aaacgcattg
      181 caccatcgcg accatagttg tgggcttcgg tggcgtgggg gcgattgacg gggagatggg
      241 tatggttgac gccaagccgg taccggtggg cgtctccata ggcaaacagt cggccctgca
      301 gcatcttgtc tggtgatggg ccaatgccag ggacaaaatt cgccgggttg aaggctgatt
      361 gttcaacgtc ggcgaaatag ttgtccgggt tgcgattgag caccagtttt ccaacttcga
      421 tcagtgggta gtcggcatat ggccagactt tggtcaaatc aaatgggttg aaccggtagt
      481 gtgccgcatc agccaccggc ataatctgca ttttgagcgt ccaggatgga aaatcacccc
      541 ggtcaatcgc gtggagcagg tccaggtgat ggaaattcgg gttttcacca gcaatccggg
      601 cggcttcagc cgaaggcaga cattttattc cctgatcggt cttgaagtga tatttcaccc
      661 aaaaggcttc accctgggca ttcagccatt gaaacgtgtg cgacccaaac ccgtccatat
      721 ggcggtatga gtaagggatg ccgcgatctc cgtaaagcca ggtgaactga tgggtcaatt
      781 ccggcgaatg agaaaagaaa tcccaggcgt tgttgggatc ctgcacgtgg gtgtagggat
      841 cgcgtttctg tgaatgaatg aaatcaggga atttctgtgg atcccgaata aaaaagatcg
      901 gtgtgttgtt gcccaccaga tcatagttgc cttcttcggt ataaaacttg acggcaaacc
      961 cgcgcggatc acgcaccgta tcggctgacc caagttcgag cgctacggtt gaaaagcgca
     1021 ggaagacttc ggttcgtttg ccaatttcac tcaggaattt cgctttggtc cattgggtga
     1081 catcgtgggt gacttcaaag tagccgtaag ctgccgagcc acgggcgtgg acaatccgtt
     1141 caggaatttt ttcacgatta aagcgggcca gtttttcaac caggtgatgg tcttgcagca
     1201 ggaccgggcc atttggaccg gcagtctggg aatgctggtt atcaacaact ggcgcacctg
     1261 attcatcagt caggattttg cgttcgctca tggtaagtct ccttatcaaa tcggttggaa
     1321 acaacagcaa cgtgatgttg gaaataaagg acttgtcagt accacctgcg tgagcgggtg
     1381 gggttgaaag caaacagacc cacccactga cgcgggtggt acttggactc attggttttg
     1441 aaaacgcagg gcagtgtggt
//
LOCUS       DAOQDO010001933         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102763_length_1458_cov_1.851033, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001933 DAOQDO010000000
VERSION     DAOQDO010001933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102763_length_1458_cov_1.851033"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..>1458
                     /locus_tag="PKZ53_23925"
     CDS             348..>1458
                     /locus_tag="PKZ53_23925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43550.1"
                     /translation="MFQSMLQDLRFAFRMLIKNPGFSGVAILTLALGLGANAAIFIVF
                     NAVVLRPLPYRAPDRLVTVWGKIPGNGLEKLSASVPEFGDYRGQTNIFSVLAAYSSIG
                     VNVTGGSDPQRVRSTFATADIFPLLGVTPLLGRPFLPEEDKPGSDDVVVLSYGLWQRR
                     FGGDPNILGQVMTINHQKCTIVGVMPSTFQFPSAETELWKPIAFTPDQFQVDQRGSHW
                     LRIIGRLNPGISLAQAQAEMTTVAERLQLQNPDYYESNSGWGITVSALQEEQTREVRP
                     TLVLMLAAVGFLLLIACANTGNLVLARVTMRRRELATRMALGAGQWRLIRQTATESMV
                     LVTIGSGLGLVFSVWGKSGLVAFLQASNVPGLDDVG"
BASE COUNT          326 a          359 c          402 g          371 t
ORIGIN      
        1 tggtaatgtg agagtcatac aaaatctcct gataagtgct cattcaactg tgcatttaac
       61 cgtacggtta ttggcgatga atttcaacac tggcttcgtt ctcaggctgt gggccgtttg
      121 tctttgacca ataagcaacc caaatgccgc cccccagtct tccgcttccg ggatagaaac
      181 gttccagaaa cgaacactgg ttgtgactct gtttccctga atcgccaatg atatcaaagg
      241 gttccaggaa ggttatgcct gcggcacgag acttgtatcg ttcagaggat tcacctattt
      301 ctctcatcaa tcaccacaca attgagcttc aaaaggaaga gggcgttatg ttccaatcca
      361 tgttgcagga cttgcggttt gcgtttcgga tgctcatcaa aaatccagga ttttcagggg
      421 ttgccatact cacattggcc cttggcctgg gtgccaacgc cgctatcttc atcgtgttta
      481 atgctgtggt gttgcgtccg ttgccgtacc gtgcgcccga ccgtctggtg accgtgtggg
      541 gaaaaatccc aggcaatggt ctggaaaaac tgagtgcctc agtacctgaa tttggggact
      601 accgggggca aacaaatatt ttctcagtac tcgcagctta tagttcaatc ggtgtgaatg
      661 taactggtgg gagtgacccc caacgagtga ggagcacctt tgccaccgca gatatatttc
      721 cgctgctggg tgtgaccccg ttgttgggcc gcccgtttct gcctgaggaa gataaacctg
      781 ggagtgacga tgttgtcgtg ctcagttacg ggttgtggca acgtcgcttt ggtggagacc
      841 caaacattct tgggcaagtg atgaccatca atcatcagaa atgtacgatt gtcggcgtga
      901 tgccttcaac tttccagttt ccgtcggctg aaaccgaact gtggaaaccg atagctttca
      961 cgcctgatca atttcaagtt gaccagcgtg gttcccactg gctgcggatc atcggacgcc
     1021 tgaatcccgg catttcactg gcccaggctc aggccgaaat gacaacggtt gccgaaagac
     1081 tgcaactaca aaatccggac tattatgaat caaacagcgg gtggggcatt accgtttcgg
     1141 cgcttcagga agaacaaacg cgagaggtcc gcccaaccct ggtgcttatg ctggcagcag
     1201 ttggattttt gctcctgatt gcctgtgcca ataccggaaa tctggtgctg gcccgcgtga
     1261 cgatgcgccg acgggaactg gcaacgcgga tggctctggg cgccgggcaa tggcggttaa
     1321 ttcggcaaac ggcaaccgaa agtatggtcc tggtcacgat tggaagcggg cttgggctgg
     1381 tattttctgt ttggggaaaa agcgggctcg tcgcattttt gcaggcttcg aacgtgccag
     1441 ggttggacga tgttggct
//
LOCUS       DAOQDO010001934         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102825_length_1457_cov_2.919401, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001934 DAOQDO010000000
VERSION     DAOQDO010001934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102825_length_1457_cov_2.919401"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1302
                     /locus_tag="PKZ53_23930"
     CDS             334..1302
                     /locus_tag="PKZ53_23930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNJ43551.1"
                     /translation="MHAMILAAGFGTRLWPLTIDRTKPAIPVLNRPLVAYAVEYLREA
                     GIQEMVVNLHHQGESVSEALGDGSQFGCRIQYSDEADQILGTSGALDHARHLLEHGPF
                     VVMNGKVITNIDLRPAIDLHVSQKALATLVLRPNTSRERFSRVEVDDRGWVTRFAGFP
                     APNDGIEEPLMFTGIQILDPAIFEFIPRGVFSHSTTDVYPQAMAQGLPVVGYVSRNPH
                     EYWYEFSTLRRYLDLSLHLHHQSTVIGEDCQIDPSAYITDSVIWKQVQIGPGSRIHRA
                     IIGDNVVLPAESNLEQVVVVRADKLSPEELEKHQAHIFHDNLVVEF"
BASE COUNT          343 a          418 c          349 g          347 t
ORIGIN      
        1 gcaatcctga cgaagaaccc aaagctgagt ctcccaaaca accagcccca gaggccaaac
       61 cagccgataa gctgaaagac atctcaggat catttctcaa tcggctgttt cgcagagggt
      121 aacatcgttg gggctgaggg cttggggctg aagactcgca agctcggggc tgaagataat
      181 tgggcttcgg gctgaattac ccgttgtctt caacccgaag ttttcagccc gacttcttca
      241 gcccgacttt ttcagcccga ctttttcagc ccgatttctt cagcccgact ttttcagccc
      301 aaagttttca gctcccgacc cattaccacc tttatgcatg ccatgatttt agcggctgga
      361 tttggaactc gcctctggcc gctcaccatt gatcgcacca aacccgcgat tcccgtttta
      421 aaccgtccgc tggttgcata tgccgttgag tatcttcgcg aggctggcat tcaggaaatg
      481 gtggtgaatc tgcaccatca gggcgaatcc gtcagcgaag cactgggcga tggcagccag
      541 tttgggtgcc gcattcaata tagcgatgaa gccgatcaaa tcctggggac gtccggggcg
      601 cttgatcatg cccgccatct gcttgaacac ggcccatttg tcgtcatgaa cggcaaggta
      661 attaccaaca tcgacctgcg accagctatt gatctgcacg ttagccagaa ggcactcgct
      721 accctggtgc tcaggccaaa taccagccgc gaacgattca gccgagttga agttgatgac
      781 cgtgggtggg tcacccgatt tgctgggttc ccagcgccaa atgatggaat tgaagaacca
      841 ctcatgttta ccggaattca aatcctggat ccggccatct ttgagttcat cccacgcggc
      901 gttttttcgc actccaccac tgacgtttac cctcaagcta tggcccaggg actaccggtt
      961 gtcgggtatg tttcccgtaa tccgcacgag tactggtatg aattcagtac cttgcgccgc
     1021 taccttgatc tcagcctgca cctgcaccat caaagcactg ttattggcga agactgccag
     1081 attgaccctt ccgcctacat tacggattca gtcatctgga aacaggtcca gattggacct
     1141 ggttcaagaa tccaccgcgc aatcatcgga gacaatgtgg tgctcccggc tgaaagcaac
     1201 cttgaacagg tagttgtggt acgggcagac aaactttcac ctgaagaact tgaaaaacat
     1261 caagcccata tcttccacga taacctggtg gttgagtttt aactggaaga aacgcgagtt
     1321 gtaacttcac ggctttggac tgacgattcg ctcctcacga cttcacatct ccccatgctg
     1381 tcagtcacca tcatcaccca caatgaagct cacaatctgc cggacgccct ggcatcggta
     1441 gcctgggccg atgaaat
//
LOCUS       DAOQDO010001935         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102949_length_1456_cov_4.121342, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001935 DAOQDO010000000
VERSION     DAOQDO010001935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102949_length_1456_cov_4.121342"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..>1456
                     /locus_tag="PKZ53_23935"
     CDS             344..>1456
                     /locus_tag="PKZ53_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43552.1"
                     /translation="MPAMIRLLLFAFLTGACLLLTAKLTQPASFHSVAGYKAPTLQAS
                     SGSRSKARLNQLSSEEKYQTYLKKKAERERLSKRPRLASGPLAAPANDTCASAEIIPG
                     TGPFPYTTSIISVVEATSDPSDPAITCGGDPMHSVWYSFTPQSDGVFTFSACGTASDF
                     DSIFGLYTSTGGCGTAFTQVACNDDSCGLQSQVSAALQTGVTYYLVVYSFGEVSAGSD
                     TLQVRVLPPAEPATNDECSTATVIAGAGPFPSATAPVGVSTASMSPTDPDPSCTVEGT
                     PPNIWYTFTPAVSGNYFISACGNETLTNTGNAISFFTSTGGCAGPFTEVFCFNNDNCG
                     SQQESGTVPLTGGTTYFILVELEGTLAADSVLQLLVD"
BASE COUNT          305 a          419 c          337 g          395 t
ORIGIN      
        1 accggatttt gtttgggagg taaatcagaa aaaccactga gtcagtcccc ccgcgtcagt
       61 gggtcggtcg gttttccaac tccactcgct cacgcaggtg gtactgacca gtcctttgtc
      121 ctacgctgtc aacccccttt cctttcaggc ttgaatttca cgagcctgaa gctgtaaagt
      181 cgtaactacc ttctctgatt cccgtctcat tccttgatca aatctcctgt ggtgtaattt
      241 ttttcctcct ggtttcatct ctccgtgaac caaagctcat accaggttta acttgcttta
      301 cttgaaccaa tccaatccat tcctttttgt tttgggagtt cctatgcctg ctatgatacg
      361 gcttctgttg ttcgcctttt taacgggtgc ttgtctcctt ctgacagcga aactgaccca
      421 accagcttcc tttcattcag ttgcaggtta taaagcccca actcttcaag ccagttccgg
      481 gtcacgttcc aaagccagat tgaatcagct ctcgtctgag gagaaatatc aaacctacct
      541 gaaaaagaaa gctgaacggg aacgactgtc aaaacggcca cgccttgctt ctggcccgct
      601 ggcggcgccg gccaacgaca cctgtgcttc cgccgaaatc attcctggca ccgggccttt
      661 cccttatacc acctccatca tttcagtcgt tgaagccacc agtgatccgt ctgacccggc
      721 aatcacgtgt ggtggcgatc cgatgcacag cgtctggtat tcctttacgc ctcaatcaga
      781 tggcgttttc actttttctg cctgtgggac agcctcggat tttgattcca tttttgggct
      841 ctataccagt accggcggct gtggtaccgc ttttacccag gttgcctgta acgatgacag
      901 ttgtgggctg caatcacagg tttcggcagc actccaaacc ggagtgacct actatctggt
      961 ggtgtactcg tttggtgagg tgtctgctgg ctctgacacg ttgcaggtgc gagttctgcc
     1021 accggcggaa ccggcgacca acgatgaatg cagcaccgcc acggtcattg cgggtgcggg
     1081 cccattcccg tccgcaaccg ctccagtcgg ggtgagcacg gcttcaatgt ccccaaccga
     1141 tccggatcca tcctgtacag ttgagggaac cccacccaac atctggtaca cctttacccc
     1201 agcggtgagt ggaaattatt tcatttcggc ttgcggaaac gagacgttga ccaatacagg
     1261 aaacgccatt tcattcttta ccagcacggg aggctgcgcc ggtccattta ccgaagtttt
     1321 ctgcttcaat aatgacaact gtggatctca acaggaatca ggaacggtcc ctctcactgg
     1381 aggaacaact tatttcattc tggttgaact ggaaggcacc ctcgctgccg atagcgtgct
     1441 tcaacttctg gttgat
//
LOCUS       DAOQDO010001936         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102954_length_1456_cov_3.542470, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001936 DAOQDO010000000
VERSION     DAOQDO010001936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102954_length_1456_cov_3.542470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1456)
                     /locus_tag="PKZ53_23940"
     CDS             complement(<1..>1456)
                     /locus_tag="PKZ53_23940"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ43553.1"
                     /translation="TLRRIIGHFQTLVSEVIIQADRSIRRLPLLTQPELDQQLIQWNE
                     TTRLYPVNRCLQALFMEQVTQTPDAIAVQFGDLGDHLLSPPTLTFRQLHDQAEHLAQL
                     LRAAGVGPEVLVGLCIERSLEMVVGVLGILKAGGAYVPMEPGYPLERLQFMVEDTQMP
                     VVLVLSKDANRFAEMTVTTFCLDALPEFIQVEPAEEKPVTQPATSLAYVLYTSGTTGK
                     PKGVQIEHRQIVNYVFGVSERVGFEPGMSYAMVQPLTVDSCKTVLFPPLLFGGCLHVI
                     TKELSLNPEALSQYFDHHRIDVLKIAPSHLAALQIPPFTQKLLPRKRLVVGGEGSTWD
                     WIHSFDELSDPACLFFNHYGPTETTVGVLTNPIQKGRREHSSPNLPLGKPLPNCTMFI
                     LDQFLQPVPVGVVGELFIGGDCVARGYLNRPNNSGFRVPGSGFRVPESEFVHQLKIQK
                     VKIPTENSEENLSLHDNSGFRVPGSGFRVPESEFV"
BASE COUNT          365 a          366 c          405 g          320 t
ORIGIN      
        1 gaacgaattc tgattccgga acccggaacc cggaacccgg aacccggaac ccggagttat
       61 cgtgaaggct caagttttcc tcagaattct cagtgggaat tttgactttt tgaattttga
      121 gttgatgaac gaattctgat tccggaaccc ggaacccgga acccggaacc cggaacccgg
      181 aattatttgg tcgattcaga tagccccgag ccacacagtc accaccgatg aataattctc
      241 cgacaacgcc caccggcacg ggctgtaaaa actgatccag aataaacata gtgcagttgg
      301 gcaatggttt gcccagtgga agatttgggc tgctgtgctc gcggcggcct ttttgaatcg
      361 gattggtcaa aacgccaacc gtggtttcgg ttggaccata gtggttaaag aacaaacagg
      421 cgggatccga gagttcatca aaggagtgga tccagtccca ggtggaacct tccccaccca
      481 ccaccagccg ttttcggggc aacagctttt gggtaaaggg cgggatttga agcgcggcca
      541 ggtgcgaagg cgcaattttc agcacatcaa tgcggtggtg gtcaaaatac tggctgaggg
      601 cttcaggatt gagggaaagc tctttggtaa tgacgtgcag acagccccca aagagtaagg
      661 gtggaaataa aaccgttttg caggaatcca ctgtgagcgg ttggaccatg gcatagctca
      721 ttccaggttc aaacccaacc cgttctgaaa ccccaaacac atagttcaca atctggcggt
      781 gttcgatctg gacgcccttg ggtttgccgg ttgtgccgga ggtatataaa acataggcca
      841 gtgaggttgc cggttgcgta actggttttt cttcagccgg ttccacctga atgaactcag
      901 gaagggcatc caggcaaaag gtcgtcaccg tcatttcggc aaaccgattc gcgtctttgg
      961 aaaggaccag taccacgggc atctgggtgt cttcgaccat aaactggagt cgttcaagcg
     1021 gatatcccgg ctccattgga acataggcgc cgcccgcctt gagaatgcca agcaccccga
     1081 ccaccatttc cagcgagcgc tcaatacaca ggccaaccag cacctctgga ccaaccccag
     1141 cggccctgag caattgggcg aggtgttcgg cctggtcatg taattgccgg aacgtcagtg
     1201 tgggcgggga taagagatgg tcgccaaggt ccccaaattg aactgcaatt gcgtctggag
     1261 tttgtgtaac ctgctccata aagagggctt gaaggcaacg gttaaccggg tacagacgtg
     1321 tagtctcgtt ccactgaatg agttgctgat caagttcagg ctgcgtcagc aatggaagtc
     1381 gccggataga tcggtctgcc tggatgatga cttcagaaac cagcgtttgg aaatgaccaa
     1441 ttattcgacg cagggt
//
LOCUS       DAOQDO010001937         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_102968_length_1456_cov_2.843683, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001937 DAOQDO010000000
VERSION     DAOQDO010001937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102968_length_1456_cov_2.843683"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1456
                     /locus_tag="PKZ53_23945"
     CDS             <1..>1456
                     /locus_tag="PKZ53_23945"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ43554.1"
                     /translation="AYVGQWEDRLIAILKEARRLKLVLYFDDLLGLFMAGQSSTSSLS
                     VADVLKPYIERRDVRILAEITPESLRVLREKDRSLADLFHLLFVEPTNENETLEVVID
                     CKRQFELRHQCTFSLDSLPVVIDLQRRFVKEAVFPGKAAGFLRQLAVKHTGHLINRQA
                     VLREFQASSGLSLAYLDDEVKLERKDIFDTLRQLVIGQETAVTAVADVVSVAKAKLSD
                     PTRPLAAMLFLGPTGVGKTHCAKVIARYLFGHEDKLLRFDMNEYLTPAAVSRLIGTFD
                     QPEGLLTSAIRRQPFAVVLLDEIEKAHPDMFNLLLQVMGDGRLTDALGRTTDFSNAIL
                     ILTSNLGVREAQTSLGFHTDGSHETAAYLTAAQKFFKPEFFNRLDRIIPFERLSRPEV
                     ERIAQGLFGEILHREGLLRRKATLYVDPRAMEAIISLGYHPQLGARALKRSLERNLIQ
                     PVAARLAALPLDSLTLISVLPQPEGFGVAVQAIEP"
BASE COUNT          313 a          402 c          387 g          354 t
ORIGIN      
        1 ggcctatgtt ggtcaatggg aagatcggtt gattgcgatc ctgaaagaag ccaggcggct
       61 caaactggtc ctctattttg acgacctgct cggtttgttt atggctggtc aatccagcac
      121 ttccagcctg tcggtggccg atgtgctcaa accctatatc gaacggcggg acgtgcgcat
      181 cctggctgaa atcacgccgg aatccctccg ggtcctgcgt gaaaaagacc gctcgctggc
      241 tgacctgttt catctgctgt ttgttgagcc aaccaatgaa aacgaaacac ttgaggtggt
      301 cattgattgt aaacgtcagt ttgaactccg ccaccagtgc accttttcgc ttgattcact
      361 gccagttgtg attgaccttc aacgccggtt tgtcaaagaa gcagtgtttc cgggcaaagc
      421 ggctggtttt ttgcgccaac tggcggtcaa gcataccggt cacctcatca accgccaggc
      481 agtcctgcgt gaatttcagg ccagcagcgg cctgtcgctg gcgtacctgg acgatgaagt
      541 caaactggaa cgcaaagaca tttttgacac cctgcgccaa ctcgtgattg gtcaggaaac
      601 ggctgtgacg gcggtggcgg atgtggtttc ggtggctaaa gcaaagctga gcgaccccac
      661 ccgcccgctg gcggccatgt tgtttctcgg tccgaccggg gtgggcaaga cccactgtgc
      721 caaagtcatt gcccgctatt tgtttggtca tgaagacaaa ctgctgcgct ttgatatgaa
      781 cgagtacctg actcccgcag ccgtttcccg gctgattggg acatttgacc agcccgaagg
      841 acttttaacc agtgccattc gccgccagcc atttgccgtc gtgctcctgg atgaaatcga
      901 aaaagcccat ccggatatgt tcaatttgct gctccaggtc atgggagatg gacgattgac
      961 cgatgccctt gggcgaacaa ctgatttttc gaatgccatt ttgatcctca cctcaaacct
     1021 gggcgtgaga gaagcccaga cgtcactcgg atttcatacc gatgggagtc acgaaacagc
     1081 agcctatctg acagcagctc agaaattctt caaacctgaa ttcttcaacc ggctggatcg
     1141 gattatcccg tttgaacgcc tttcccgacc tgaagtcgag cggattgccc agggattgtt
     1201 tggggaaatt cttcatcgtg aaggacttct gcgccgaaaa gcgacgttgt atgttgatcc
     1261 acgggcaatg gaagccatta tttcccttgg ataccatccc cagcttggtg cccgcgcgct
     1321 caagcggtca ctggaacgca acctgattca acccgtcgct gcccggctgg cggccctccc
     1381 gcttgattca ctgacactga tcagtgtgtt gcctcaacct gaaggatttg gcgttgccgt
     1441 tcaggcgatt gaaccc
//
LOCUS       DAOQDO010001938         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103092_length_1455_cov_4.136429, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001938 DAOQDO010000000
VERSION     DAOQDO010001938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103092_length_1455_cov_4.136429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1455
                     /locus_tag="PKZ53_23950"
     CDS             <1..>1455
                     /locus_tag="PKZ53_23950"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNJ43555.1"
                     /translation="GVLRGFQKIFDDGSKRFTAGLKKKSSFVILGGSLAQSGPVYIVQ
                     GLATGLSIRKAIGASVICALDAFNLEPVVKSLRKAARGLKIIIAADNDHTKAQNLNPQ
                     TGLPIGNTGLRKAHRTALKYRCLVASPGFRPDDPGTDFNDLQQAYGLNAVRDDLDMAS
                     RPNPALAYQGERESQIARKAKAFSRCELIRMESQYLSAESLQAAFERPDVKTIVAASP
                     IGTGKTEAVAQLISGLDPNERVLYISHLVSLNADAAARLGLEMYSDYKGHRADGDCKL
                     TELPRVAVCLNSLPMLVDGGRIQPFEVIVVDEIEQLIRRLTTPIDHKRLVIDVLKYLV
                     QHAKKLILLDAHIGKVTLELLDLWRPDGGVILWNTHQPATSRTVQLYPDKETLVETAV
                     NAVATGEKVFFCANSKNEARRVFKLMQKVGTAGHGLYISGDNRGDADVTNFLADVNKQ
                     ATSYDFIVVSPAICTGVNIDNGHFTWVGGAFRCDV"
BASE COUNT          373 a          356 c          410 g          316 t
ORIGIN      
        1 gggggtactg cgcgggttcc aaaagatctt tgatgatggc agcaagcgat tcacggctgg
       61 actgaagaaa aaaagctcat ttgtaatact tggggggtca ctcgctcaat ccgggccggt
      121 ttacatcgtg caagggttgg ctaccgggct ttcaatccgt aaagcaatcg gggcaagcgt
      181 aatttgcgcc ctcgatgcgt tcaaccttga gcccgtggtg aaatcgctcc gaaaagccgc
      241 acgcgggctg aaaatcatca tcgcggcgga caacgatcac accaaagccc aaaacctaaa
      301 tcctcaaact ggattgccaa tcggaaacac cgggctgaga aaggcacacc ggacagcact
      361 caaataccgg tgtttggtgg catcaccagg gttcagaccc gatgatccgg gaacggattt
      421 caacgacctg cagcaagcat atggactgaa cgccgttcgg gatgacctgg atatggcatc
      481 gcgaccaaac ccggcactgg cctaccaggg ggagcgggaa agtcagattg cgcgcaaagc
      541 gaaggctttt tcacggtgcg agttgatccg gatggaatcc caatacctgt cagccgagag
      601 tttgcaggcg gccttcgaaa gacccgatgt aaaaaccatt gtggcggcat cgccaatcgg
      661 gaccggcaaa actgaagcag ttgcccaact tatcagcggg ttggatccaa acgagcgggt
      721 tctgtatatc agtcaccttg tgtcgctgaa tgcggatgca gcggcacggt tggggctcga
      781 aatgtactcc gattacaagg ggcaccgggc agatggtgac tgcaagttga cggaactccc
      841 acgggtggcg gtgtgtctaa actcgcttcc gatgctggta gatggtgggc gcattcaacc
      901 ctttgaagta attgtcgttg atgaaattga gcaattaatc agacgattga ccaccccgat
      961 tgaccacaaa cggctggtga ttgacgtctt gaaatatttg gttcagcacg ccaaaaaact
     1021 gattttgttg gatgcacata ttggaaaagt cactcttgaa ctcctggact tgtggcgacc
     1081 agatggcggg gtgatcctct ggaatactca ccagccagcc accagccgaa cagttcaact
     1141 ttaccctgac aaagagaccc tggttgaaac agcggttaat gcagtggcaa ccggtgaaaa
     1201 agtcttcttc tgcgcaaact caaagaatga ggcacggcgt gttttcaaac tcatgcaaaa
     1261 agttggtacc gctggacacg ggctttacat ttcgggagac aatcgcggtg atgcggatgt
     1321 gacgaacttc cttgcggacg tcaacaagca ggccacgagc tatgatttca tcgttgtcag
     1381 cccggctatt tgcactgggg tcaacattga taatgggcat ttcacatggg tgggtggtgc
     1441 gtttcgctgc gatgt
//
LOCUS       DAOQDO010001939         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103101_length_1455_cov_3.406429, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001939 DAOQDO010000000
VERSION     DAOQDO010001939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103101_length_1455_cov_3.406429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PKZ53_23955"
     CDS             <1..1054
                     /locus_tag="PKZ53_23955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43556.1"
                     /translation="MQADVAARVARSLEVELLPQSQPARGHSPHPEAYDAFLKGCYLV
                     SKDTLPDFERSLGYFEQAIQKDPAFAAAYARLAETQVLIAIWRNTTSPELVAKAKMAA
                     EKAVALDPMLPEALAALGAVNFWLEWNWPEAEKNITQALAFNPSDPAVHLLYASLLLT
                     KEQIASARREIQQALALDPVSRLTNGIAAYCYLRAGQLDEAVTQSRRMLELEPDSAAG
                     YSCLTSAYFFQGNYHMAQELWHKRLLQLGGNNTWLNQLHSDEPERGIADLHRAMLEQM
                     MKRRRQGEPVPASSIASIHFNLGNQEQGIEWLEKAVASREPLVVFLKTHPTYARLRTD
                     SRFQKLVHRLGLDLSQ"
     gene            1253..>1455
                     /locus_tag="PKZ53_23960"
     CDS             1253..>1455
                     /locus_tag="PKZ53_23960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43557.1"
                     /translation="MAERGVDFAHLQDLVSLPYIEDQKSDDPEQYRIVGFANGMMATF
                     IVEYRFDELGEIMWVVTAWQSTTS"
BASE COUNT          362 a          343 c          392 g          358 t
ORIGIN      
        1 gatgcaggct gacgtggctg cccgtgtggc gcgctcgctg gaagtggaac ttctgccaca
       61 gagccagccg gcgagggggc attcaccaca tccagaagcg tatgacgctt ttttgaaagg
      121 gtgctatctg gtttcaaaag atacacttcc ggattttgag cgcagccttg gatactttga
      181 gcaggcgatt caaaaagatc ccgcttttgc cgccgcttat gcccggctgg ctgaaaccca
      241 ggtactgatt gcaatttggc gcaatacaac ttctccggaa ctggtcgcga aagccaaaat
      301 ggcggctgaa aaagcagttg cgcttgatcc aatgctcccg gaagccctgg cggcgttggg
      361 cgccgtcaat ttctggttgg agtggaactg gcccgaagcc gagaaaaata tcacccaggc
      421 gctggctttc aacccaagtg atccagccgt gcatttgcta tatgcctcgc ttttattaac
      481 caaagaacaa atcgcatctg cccgtcggga aattcaacag gcactggcgc ttgacccggt
      541 ttccaggcta acaaatggaa ttgcggccta ttgttatttg cgggctggtc agcttgacga
      601 ggcagttact caaagccggc gcatgctcga acttgagccg gactctgcgg ctggttattc
      661 ctgtttgacg tcagcttatt tcttccaggg aaattatcac atggctcagg agctatggca
      721 caaacgcctg ctgcaactcg gaggtaacaa tacatggtta aaccagttac attctgatga
      781 gcctgagcgt gggattgccg atttacatcg agcgatgctg gagcagatga tgaagcggcg
      841 ccggcaaggt gagcctgttc cagccagttc aatcgcttca atccatttca acctgggaaa
      901 ccaggagcaa gggattgaat ggctcgaaaa ggcagttgcc agtcgtgaac cactggttgt
      961 gtttttgaag acccatccga cttatgcccg gctacgcacc gactcacggt ttcaaaagct
     1021 cgttcaccgc ctggggctgg acctcagtca gtaactgtga gccaaaagaa atgccagccg
     1081 gaggctcgat tgaggaggct ccttcggctg gcagagaact tatgagtggt tgctgtgata
     1141 gtttttctaa agtaattgat agtgcccaat gagttgttgt atataaaagg tataaaaatt
     1201 atatcttatt attatgcgaa tcaaatggga tgaaccaaag cgccaattag tcgtggcaga
     1261 acgcggagtg gattttgcac atttgcagga cctggtaagc ctgccttata tcgaagatca
     1321 aaagtctgat gatcccgagc aatatcgaat cgttggattt gcaaacggaa tgatggccac
     1381 gtttattgtt gaatatcgct tcgacgagtt gggggaaatt atgtgggttg tcactgcctg
     1441 gcaatcaacc acatc
//
LOCUS       DAOQDO010001940         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103121_length_1455_cov_2.707857, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001940 DAOQDO010000000
VERSION     DAOQDO010001940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103121_length_1455_cov_2.707857"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PKZ53_23965"
     CDS             <1..968
                     /locus_tag="PKZ53_23965"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNJ43558.1"
                     /translation="KHQAPSTKHQKPSTKNQAPKTRHPSPEVMADTVIWAALSQLLIA
                     TLHGFFGAWLAVRMVFRPRKAIIVWGIQLPFTPGMIPAERERFLTTFAQVIAERVLTV
                     ETIADELFRLGIRREMEVVSARQYAEQTGSDAFLHDIAERMVQVIEDTGNNRQVAERL
                     DISVARGVIAAVDEKYGLLGQTVAKILVEAGLVRKVLAASLRDLAKEISQNPLSRAAL
                     LEAIAAAGHQIFPVRELKDELDGLPLVTPDLSAVDRFVISLSQRLDMERILKEQLMAF
                     SDEMIEDLVYQAAGRELKMIIRFGAVVGFLIGLVQALLVVVSRWP"
     gene            complement(1049..>1455)
                     /locus_tag="PKZ53_23970"
     CDS             complement(1049..>1455)
                     /locus_tag="PKZ53_23970"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNJ43559.1"
                     /translation="PLSLAATFVVRPAMLDNYRAHWWGLVFPMLVVGGLVGLEYFWRT
                     GKDGMVFLSSTTYLVGMLGGAAFGLFPTVLPASTDVAHSLTIYNASTGEYGLSVGLIW
                     WCIGMLLASGYFAYLYRSFQGKVLVEDSQTHG"
BASE COUNT          376 a          374 c          377 g          328 t
ORIGIN      
        1 ccaagcacca agcaccaagc accaagcacc aaaaaccaag caccaaaaac caggcaccaa
       61 aaaccaggca cccgtcacca gaggttatgg ccgacaccgt catttgggca gcgctttccc
      121 agttattgat tgccacgctc cacggttttt ttggagcctg gcttgcagtc cgaatggttt
      181 ttcgtcctcg gaaagcaatt attgtgtggg ggatccaact gccctttacc ccaggcatga
      241 ttccggctga acgcgaacgc tttttgacca catttgctca ggtgattgct gaacgagtgc
      301 tgaccgttga aacaatcgct gatgaattgt ttcgattggg gattcgccgg gaaatggaag
      361 tggtgtcggc cagacaatat gccgaacaaa ccggaagcga tgccttttta catgatattg
      421 ctgaacgaat ggtccaggtg attgaggata ccggcaacaa tcgtcaggtg gccgaacgcc
      481 tggacatttc tgtggcacgt ggggtcatcg ccgctgttga cgaaaaatat ggattgctcg
      541 ggcaaaccgt agcgaaaatc ctggttgaag ccgggttggt gcgcaaagtt ctggccgcct
      601 cgctccgaga tctcgccaaa gaaatcagtc aaaacccact ttcacgagct gccttgttgg
      661 aagccattgc tgccgccggt catcaaattt tcccggttcg agaactcaag gacgagctgg
      721 atggactccc cctggtcaca ccagatctct cagccgttga tcggttcgtg atttcactca
      781 gccagcgact ggatatggag cggattttaa aagaacaatt gatggcgttc tctgatgaaa
      841 tgatcgaaga tctggtctat caggcggctg gtcgcgaact caagatgatt attcgatttg
      901 gggccgtagt cgggtttttg atcggattag ttcaggcgtt gctggtggtt gtgagccgct
      961 ggccctaatg gaatgaagaa ggaagaattg agaatgaaga atgaaatcaa ttcgaaatct
     1021 tcagccccag gccctcagcc ctggtttttc agccatgagt ttgcgaatct tccaccaaaa
     1081 ccttgccctg aaatgaacgg tagagatagg caaagtaccc tgaggcaagg agcattccga
     1141 tgcaccacca gatgagcccg acactgaggc catattcgcc agttgaagca ttgtaaattg
     1201 tcaggctgtg cgccacatcg gtactggcag gcagaactgt cggaaaaagt ccaaaggcgg
     1261 caccaccaag cattcccacc agataggtgg ttgatgacag aaacaccatt ccatcctttc
     1321 cggtcctcca aaaatattcc agtccgacga gtccacccac aaccagcatt ggaaacacca
     1381 gtccccacca gtgtgcccgg taattatcga gcatggcggg tcgaaccacg aatgtagccg
     1441 ctaagctcag tggtg
//
LOCUS       DAOQDO010001941         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103135_length_1455_cov_2.485000, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001941 DAOQDO010000000
VERSION     DAOQDO010001941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103135_length_1455_cov_2.485000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1058)
                     /locus_tag="PKZ53_23975"
     CDS             complement(48..1058)
                     /locus_tag="PKZ53_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ43560.1"
                     /translation="MVEPESLRDDYDSPWKEALDEYFKDFLELLFPMVHDGIDWSIPP
                     QSRDKEFQKVTATAQMGRQVADKLKEVQTLDGVKRWVLVHVEVQSQWEADFPERIFRF
                     DARLFDEFGKHVVSLVVLGDSNPDWVPKEFGWAFWGYRMVVQFPTVKLVKLNVDGLEQ
                     SQNRFAPIVLAHLKTLETKHAPQTRREWKFRIVRSLYERGYSADQVRKLFRLIDWFMQ
                     LPTDLENQFQRDLEEYEEEHHMPYVTSIERFAEARGEARGEARGEARGEARGEARGRV
                     QSKIEMLLLTLKLKFGELTPDQVTTIQTIQDEDQLMVLYQKAVLAGSPEDLAPFLEAL
                     NH"
BASE COUNT          348 a          367 c          319 g          421 t
ORIGIN      
        1 agcatttcag aaaaaggttc agcgtatcgt cttgaaccag agagagttta gtgattgagt
       61 gcttccaaaa acggggccag gtcttcaggc gaccctgcca gaacagcttt ttggtacaga
      121 accatcaatt gatcttcatc ctgaattgtc tgaatagtgg tgacctgatc tggcgtcagt
      181 tcaccaaatt tgagtttcaa ggtgagcaaa agcatttcaa tttttgattg tacccggcca
      241 cgggcttcac cacgggcttc accacgggct tcaccgcggg cttcaccacg ggcttcaccg
      301 cgagcctcgg caaaacgttc gatacttgtc acatacggca tatgatgttc ctcctcatat
      361 tcttctaaat ctctttgaaa ctggttttcc agatcggttg gtaattgcat aaaccagtca
      421 atcaatcgaa acagttttcg aacttgatcc gccgaatatc cccgttcata gagcgaacgg
      481 acaatccgaa atttccattc gcggcgtgtt tgaggtgcgt gtttggtttc aagggttttc
      541 aaatgcgcca gcacaatcgg cgcaaagcga ttctggctct gttcgaggcc atcaacattt
      601 aatttgacaa gtttgacggt tggaaactga acgaccattc tatatcccca gaatgcccat
      661 ccaaattcct tcggaaccca gtctggattt gaatctccca acacgaccag actcacgacg
      721 tgtttcccga attcatcaaa caaccgggcg tcaaatcgaa aaatccgttc ggggaaatcc
      781 gcttcccact ggctttggac ctcgacatga accagtaccc agcgttttac gccatcaagt
      841 gtctgaactt ccttgagttt atctgccacc tggcggccca tttgggcagt tgccgtcact
      901 ttctgaaatt ccttatcgcg tgattgaggc gggatactcc agtcaatgcc gtcgtggacc
      961 attgggaaga gcaattccag aaagtctttg aaatattcat caagtgcctc tttccagggg
     1021 ctgtcataat cgtcacgaag tgattcgggc tccaccattg atttctctcc tgacagaaat
     1081 gttgaaaaga aagataccag atcagcttgt gtttgattga acctccccat tttcctgtct
     1141 gaaaaaaacc ttgtcaatcc agccagttgc gtgtaagtag aagcctttct gacacagtgt
     1201 tccttcatcg gtttttcaac cattgttcct caccaaattc aattggttcg ctttgaggtt
     1261 ccgaccaatc gctgtctggt cgttcctcac gggtcaagtt cttccaaaat ccacctgttt
     1321 aaaggagttc ccttgatgac caattttttt cggaagttca tggcggggtt gtctcgtcct
     1381 accgttcttt ggctgggatg ctcgatgttg tttttttcct caactgtttt ggctgacacg
     1441 acaggcgtga ttgtg
//
LOCUS       DAOQDO010001942         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103193_length_1455_cov_1.857857, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001942 DAOQDO010000000
VERSION     DAOQDO010001942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103193_length_1455_cov_1.857857"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKZ53_23980"
     CDS             complement(<1..326)
                     /locus_tag="PKZ53_23980"
                     /inference="COORDINATES: protein motif:HMM:NF024673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNJ43561.1"
                     /translation="MKRGLVEVGHPELSIARQCELIGLARSSWYYQAGGEGADNLAVM
                     RRLDELYTAHPFYGVRRMTAALRQEGYAINPKRVRRLLRLMGLAAIYPKPNLSCPDLT
                     HQVYPYL"
     gene            complement(328..618)
                     /locus_tag="PKZ53_23985"
     CDS             complement(328..618)
                     /locus_tag="PKZ53_23985"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ43562.1"
                     /translation="MAKTRKTHSPEFKAKVALEAIRGHKTINEIASEYEVHPVQIGQW
                     KKMAVEELPQAFGKRQAAQTKADEELQAALYEQIGKLQMELAWLKKKAGLAG"
     gene            826..>1455
                     /locus_tag="PKZ53_23990"
     CDS             826..>1455
                     /locus_tag="PKZ53_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43563.1"
                     /translation="MRKFRCIIACVIISIAYVIGQFSLSFPVGVSDQHDQFHSTQWKT
                     VQTKRVQFRCPSSFQVQVWEPENEEEVPSQINLLAPTEILQIEINPAQDDFALESLDN
                     NQSLLVRYDFDGETCHRHCEIYSGINFKTRSGLECRELKIKKIVDCHPENVCRPGEVT
                     EYYDVPSIFAGRFTRNGKAGCVYVIPDLGARRIPNDILWEIVQSIQISIP"
BASE COUNT          326 a          396 c          338 g          395 t
ORIGIN      
        1 agatacggat atacctggtg ggtcaggtcg gggcagctca gatttggttt tggataaatc
       61 gctgccagcc ccatcaaccg caacaagcgc cgcacccgtt tcgggttgat ggcgtaccct
      121 tcctgccgca gcgccgccgt catccgccgc actccgtaaa acggatgggc cgtatagagt
      181 tcatccaacc gccgcatcac cgccagattg tccgcccctt cccctccagc ctggtaatac
      241 caacttgacc gcgccaaccc aatcaactca cactgccggg cgatgctcaa ctccgggtgt
      301 ccgacctcga ccaacccccg cttcacttca accggccagt ccagcttttt ttttcaacca
      361 cgccagttcc atctggagtt tcccaatctg ctcatagagc gccgcctgta attcctcatc
      421 cgctttggtc tgcgccgcct gccgcttccc gaacgcctgc ggcagttcct ccaccgccat
      481 ttttttccat tgcccgattt ggaccggatg gacctcgtac tcgctcgcta tctcattgat
      541 cgttttgtga ccccggatgg cttccaacgc cactttcgct ttgaattccg ggctatgggt
      601 ttttcgtgtt tttgccatga tttttcccct cccctgggtc tcaatcataa cttatttccc
      661 tgtccagttt ttggggagca ttataggttg tcgtgtttgc gccacccaac aatggcatgc
      721 accggactct cgcagcacgg ttcatcggtc ggaacgaagg ttgttcggtg ctgtgttcgc
      781 cggtgatgcc cgccgttggg cttgtaattt ggtgttcgga ggattatgag aaaattcaga
      841 tgtattattg cctgtgtgat aatttcaata gcttatgtga taggccaatt ttcactatca
      901 tttccggttg gtgtttccga tcagcacgac caattccact ctactcagtg gaaaacggtt
      961 caaaccaaaa gggtacagtt ccgttgtcct tcaagttttc aagttcaagt atgggagcca
     1021 gaaaatgaag aagaagtgcc atcccaaata aatctcttgg ccccaacaga gattctgcag
     1081 attgaaataa atccagctca ggatgatttt gcgcttgaaa gcctggataa caatcaatcc
     1141 cttttggtga ggtatgattt tgatggagag acctgccatc ggcattgcga aatctattct
     1201 ggcattaatt tcaagactcg atccgggctt gagtgcagag agttaaaaat caaaaagatt
     1261 gtggattgtc acccggaaaa tgtgtgccgc ccaggagagg tgacagaata ttatgatgtg
     1321 ccttcaatct ttgctggacg gtttaccaga aacgggaaag caggttgtgt ctatgtaatt
     1381 ccagatctcg gggcaaggag aattcccaac gacattttgt gggaaatcgt tcaatccatc
     1441 caaatcagca tcccc
//
LOCUS       DAOQDO010001943         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103225_length_1454_cov_3.904217, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001943 DAOQDO010000000
VERSION     DAOQDO010001943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103225_length_1454_cov_3.904217"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1420
                     /locus_tag="PKZ53_23995"
     CDS             425..1420
                     /locus_tag="PKZ53_23995"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ43564.1"
                     /translation="MSHSLPYATLSYLDSSGQTHIVPINAPIFTIGRLRTNHLQINDS
                     YVSRQHAEIAYQDGHFYLRDKNSTGGCLVNGKRVSDYLLQHNDQIQLGNTNPLILVFE
                     LHDDQITTEASEMNTGEEHSTDKLMTIITHNQTRFLNTSLLDSAECITDATLNRLKNL
                     NETMRRMLAARSTQELLEILLDAVMAALPAERGVVILPPQGSNRLEIRAVRRRTENGT
                     PVQPSSTIVEHVYQENVAIRSFDAMADNRFALNSSIIQQAIHSVMCAPVSSQNRVWGV
                     CYIDNLMAHTQFNDEELEFFLALTQQAGLTMENLHLIEELRATQEKLIARTGCEF"
BASE COUNT          380 a          382 c          323 g          369 t
ORIGIN      
        1 tgtaaatctt tttgtttcct gactctcaaa cctcaggtaa acgtggtatg ctgagccgac
       61 atgatttctg ccagagcaac ccggacagta cacaccgttg cttcattcct gagtacattt
      121 tgttcctggg gtttcagcgt caacgacctc tgggtccacc tggagacctg actcctgtca
      181 ctctttttct gccgctgtcg ttgatgatca cactggtctg aagtaagagc ccaaagtggc
      241 tcactcacca gacagttgcg gatggggcag ggcaaaattc acctgcaccc ctcgcaccca
      301 acacctattg caacctgtgg gaaatctgca gcaacgtccc accctacaca atcttaacgg
      361 gagaacggcg ttacctcttg cctgtgatct ggcggggctc cacgtcgatt ttaagatgaa
      421 agagatgtct cattcccttc cctatgccac cctcagttac ctggatagtt caggacaaac
      481 acatattgtc ccaatcaatg caccaatttt cacaattgga cgattgagaa ccaaccatct
      541 tcaaatcaat gactcctatg tttcgcgtca acatgctgaa attgcctatc aggacggaca
      601 tttctatttg cgcgataaaa acagtaccgg agggtgcctg gtcaatggga agcgggtgtc
      661 agattatttg ctccagcaca atgatcaaat ccaactcggc aataccaacc ccttgattct
      721 cgtttttgaa ctccacgacg atcaaattac gactgaagcc tctgaaatga acacgggtga
      781 ggaacattca actgacaagt taatgaccat catcactcat aatcaaaccc ggtttctcaa
      841 cacctcgctc ctcgatagtg ccgaatgcat caccgatgcg accctgaacc gactgaagaa
      901 tttgaatgaa accatgcggc ggatgctggc agcccgcagt actcaggaat tgctcgaaat
      961 cctgcttgat gcggtcatgg cggccttgcc ggcggaacgc ggcgtggtaa tcttgcctcc
     1021 ccagggctca aatcggctgg aaatcagagc cgtccgacgg cgaactgaaa atgggactcc
     1081 ggtccaaccg agcagcacga ttgtcgaaca cgtttatcag gaaaatgtcg ccattcgcag
     1141 ctttgacgcc atggcggata atcggtttgc cctcaattcc agcattatcc aacaggcaat
     1201 tcactcagtt atgtgcgctc ctgtcagttc acaaaatcga gtgtggggtg tctgctatat
     1261 tgataactta atggctcaca cccagtttaa tgatgaagaa ctggaatttt tcctcgcctt
     1321 gacccaacaa gccgggttga cgatggaaaa tttgcatttg attgaagaat tgcgcgcaac
     1381 tcaggaaaaa ctcattgcca gaactggttg cgagttttga aaattgatct aaacctttgt
     1441 tttgtatatg ttag
//
LOCUS       DAOQDO010001944         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103477_length_1452_cov_3.405870, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001944 DAOQDO010000000
VERSION     DAOQDO010001944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103477_length_1452_cov_3.405870"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1138
                     /locus_tag="PKZ53_24000"
     CDS             167..1138
                     /locus_tag="PKZ53_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNJ43565.1"
                     /translation="MATVLWLQGGACSGNTMSFLNAEEPSVCDLITDFGLDLLWHPSL
                     GVELGDNVQKLLRDCTSGKKPLDIFVYEGTVIMGPNGSGRFDMFADRPMKDWVVELCN
                     QAQLVVAIGDCACWGGIPATAPNPSESIGLQYMKRDRGGFLGPDYRSKMGLPVINIPG
                     CPAHPDWITQIIVALASGRVGDVALDDLQRPQTFFKTFTQTGCTRVQYFEYKQSTMEF
                     GQGTRKGCLFYELGCRGPMTHSSCNRILWNRQSSKTRAGMPCTGCTEPEFPFFDLAPG
                     TVFKTQKISGVIPKEVPNGTDHVTYMTHAAAAKVASPQWAKEDMFVV"
BASE COUNT          356 a          375 c          368 g          353 t
ORIGIN      
        1 tctcccttta ttttttcttt ccctgaaccc tgaaccctga accctgaacc ctgaatcctg
       61 tcttttccgc attttttcaa atttgttacc cgagaagatt gctcgggcag gggatggtct
      121 gtcttcaaac agctcacctc cagtgaccaa ccaaggagaa atcgccatgg caacagtttt
      181 atggttacaa ggtggcgcct gtagcggcaa caccatgtcc tttctcaatg ccgaagaacc
      241 aagcgtgtgt gacctgatta ccgattttgg actggacctg ctctggcatc cgtcacttgg
      301 cgtcgagcta ggtgacaatg ttcaaaaatt actcagggac tgcacgagcg gcaaaaaacc
      361 gctcgatatt tttgtgtatg aaggcaccgt cattatgggg cccaatggtt caggccggtt
      421 tgatatgttt gctgaccgac caatgaaaga ctgggtcgtg gaactctgta atcaggcgca
      481 acttgtggtt gccatcggtg attgtgcctg ctggggagga attccggcaa ccgccccaaa
      541 cccaagcgaa tccatcgggt tacagtatat gaaacgtgac cggggcggat ttttaggtcc
      601 cgactatcgt tcgaagatgg gcctgccggt catcaacatt cctgggtgcc cggcgcatcc
      661 tgactggatt acccagatta tcgtcgccct ggccagtggt cgcgtcggtg acgtggcgct
      721 tgatgacctg caacgaccgc aaaccttctt taagaccttc actcagaccg gctgtacccg
      781 cgttcagtat tttgaataca agcaatccac gatggagttt gggcaaggca cccgcaaagg
      841 ctgcctgttt tatgaactgg ggtgccgtgg accaatgacc cattcgtcct gtaaccggat
      901 tttgtggaac cgtcagtcaa gtaaaacccg cgccgggatg ccctgcaccg ggtgtaccga
      961 acctgaattt ccattctttg atttggcgcc cggaactgtg ttcaaaaccc agaaaatcag
     1021 cggcgtgatt ccaaaagaag ttcccaatgg caccgaccac gtaacctata tgacccacgc
     1081 cgctgccgcc aaagtcgctt cgccacaatg ggcgaaggaa gacatgtttg tggtttgaaa
     1141 aaactttggg ctgaagaaaa cgggctcagg gctgaaaact cgcaagctcg gggcgaccca
     1201 agaaataagg gatgaaataa aaccatttct tcagcccgaa attttcagtc cgatgtcttt
     1261 agcctgggtt tcaggtcttt gggcagagag gtttgcaaac ctggggttaa agacatcggg
     1321 ctgaaagact cgcaagaatt cggtaacgcc cattcataat tcataatttg gtacaaggag
     1381 tgagtatggc aacagtaagc tcaagttcca ccgccaccaa agccgttgat cttcatatca
     1441 gtcctctggg gc
//
LOCUS       DAOQDO010001945         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103496_length_1452_cov_2.805297, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001945 DAOQDO010000000
VERSION     DAOQDO010001945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103496_length_1452_cov_2.805297"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PKZ53_24005"
     CDS             complement(<1..1373)
                     /locus_tag="PKZ53_24005"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43566.1"
                     /translation="MIQPFRSVVGFGGTIWFCLQFGVPSLISVPSGCQNQEAGVPAPI
                     LRTLTGGEKHIHPLVLNANDCVQVIVDQHGIDVVVRLLGPDGDKLQEVDSLNGTDGPE
                     PLTVIVAQSGAFRIEIESLEKTARPGKYEFKLTPPHPATKLDRAEVDLGVLNAKLEQL
                     QSQGKTAQALELAQPGVARAEQVFGSSGPYVLARSLTILGGLHFAAGEYARAEPCFER
                     ARVICEQTFGVTHLETAGSLNNLAVLATEKGNFEKAESLYRQSLAISEQVVGPEHPQV
                     AAITHNLADVLEHLGRYSQAEILYRQALTIREKVFGPHHLETAATLHGLAVLYDSTGE
                     YPRAEELYQRALGIREKALGRIHPLTVATLNNLALLADKSGDLTTAERLYQQVLAINE
                     ANLGEHHPGVAVIVYNLAALYRDQGDYTKAEPLYQRALAIFEKRLGPDHPRVAACLSG
                     LALLYQF"
BASE COUNT          326 a          409 c          378 g          339 t
ORIGIN      
        1 ttgaactggt acagcaacgc cagaccagaa agacaggcag ccacacgtgg atggtcaggt
       61 cccaggcgtt tttcaaaaat ggccagggca cgttgataaa gcggttctgc ttttgtgtag
      121 tcgccctgat cgcgatacag cgccgccagg ttatagacga taacagcaac ccctggatga
      181 tgctccccga gatttgcttc attgatggcc agaacctgct ggtagagacg ttcggcagtt
      241 gtgaggtctc cacttttgtc agccagcaac gccagattat tgagggtggc gaccgtgagc
      301 gggtggatcc tgccaagggc tttttctcgg attcccagtg cccgctgata cagttcctcc
      361 gcccgagggt attcgcccgt gctgtcatat agaaccgcca gaccgtgcag ggtggcagca
      421 gtttccaggt ggtgcgggcc aaagaccttt tcccggatgg tcagcgcctg gcgatagagg
      481 atttccgctt gcgagtaccg ccccaggtgt tccaggacat cagccagatt atgggtgatg
      541 gccgcgacct ggggatgctc tggacccact acttgctcag aaattgccag tgactgccgg
      601 tacagtgatt cggctttttc aaaattgcct ttttcggtcg caagcaccgc cagattgttg
      661 agagaaccgg cggtttccag atgggtgacg ccaaacgtct gctcgcaaat caccctggct
      721 cgctcaaaac agggttccgc acgggcatat tcaccagcgg caaagtggag acctcccaaa
      781 atcgtcaaac ttcgagccag aacatacggg ccactcgaac caaaaacctg ttcagctctg
      841 gcaacccccg gttgcgccag ttccagcgcc tgcgcggttt tcccctgaga ttgaagctgc
      901 tcaagtttgg cgttgagcac accgagatca acttcagccc gatccagttt agtggctgga
      961 tgtggcggcg ttaatttgaa ctcatacttt ccggggcggg ctgttttttc cagggattca
     1021 atttcaatcc ggaaggctcc gctctgcgcc acaatcaccg tcagtggttc ggggccatct
     1081 gttccgttga gactatccac ttcctggagt ttgtcgccat ccggcccaag caaccgcacc
     1141 accacatcaa ttccgtgctg gtcaacgatt acttgcacac aatcattagc gttaagcacc
     1201 agtgggtgaa tgtgtttttc tcctccggtc aacgttcgaa ggatcggagc tggaacgcca
     1261 gcttcctggt tttgacagcc tgaaggaact gaaatcaacg atggcacacc aaattgtaag
     1321 caaaaccaga tcgtgccccc aaaccctaca acggatctga atggttgtat cacgtcttga
     1381 cctcctgact tttctgacaa tctttcaatc cggatcttcc acaagcagga ttactggcaa
     1441 acgcgtcaaa cc
//
LOCUS       DAOQDO010001946         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103524_length_1452_cov_2.293486, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001946 DAOQDO010000000
VERSION     DAOQDO010001946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103524_length_1452_cov_2.293486"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PKZ53_24010"
     CDS             <1..1296
                     /locus_tag="PKZ53_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNJ43567.1"
                     /translation="PKPALSNSTRQVRYLPSPLDIPAVEGIVPRTDANETREANDNAP
                     FSGLVFKIMGDKHVGQLAFVRIYSGTLQSGSYVLNSTRDSKERVGRVLKMHANKREEV
                     DSAGAGEIVAVAGLKNVTTGDTVCDDKKPIILEAMEFPAPVIEVAIEPKTRQDQDKLS
                     IALGRLAQEDPSFRVKVDPETNQTLIAGMGELHLEIIVDRLKREFSVDANVGRPQVAY
                     RETIRIAADGNGVFKRQTGGRGMYGHAEIKMYPGEPGSGFVFENKVVGGSIPREYIKP
                     VENGIIEAMGRGIVAGFEMVDIRVELVFGSYHEVDSNEMAFKIAGSMAFQDAAKKAKP
                     VLLEPIMSVEVEAPEEYMGAITGDLSSRRGRIEKMDERPGTKIITAAVPLATMFGYST
                     DLRSATQGRAIYTMHFKCYEEAPKSVSDEIIARVKGTAA"
BASE COUNT          380 a          347 c          402 g          323 t
ORIGIN      
        1 ccaaagcccg cgttatcgaa ctcaacgcgt caggttcggt atctgccttc accgcttgat
       61 attccagcgg ttgaaggaat tgtgccccgt actgatgcca atgaaacccg tgaagccaac
      121 gacaatgcgc cgttttcggg gctggtcttc aagattatgg gcgacaagca cgttggtcag
      181 cttgccttcg tccggattta ttcaggaacg ctccagtctg gctcatatgt gttgaactca
      241 acccgtgatt cgaaagaacg cgtgggccgg gttctcaaaa tgcacgccaa caagcgcgaa
      301 gaagttgatt cagccggtgc tggtgaaatc gtagcggtgg ccgggttaaa gaacgtgacc
      361 actggcgaca ccgtgtgcga tgacaagaaa ccgatcattt tggaagccat ggaattcccg
      421 gcgccggtga tcgaggtcgc catcgagccc aaaactcgcc aggatcagga caagctttcg
      481 attgctctgg gccgtctggc acaagaagat ccgtcgttcc gtgtcaaagt tgaccccgaa
      541 accaatcaga cgctgattgc cgggatgggg gaacttcacc tggaaattat tgttgatcgg
      601 ttgaagcgtg aattttcagt tgatgccaac gtcggtcgtc cacaggtggc ctatcgcgaa
      661 acaattcgga ttgctgccga tggaaatggc gtattcaagc gccagaccgg tggtcgcggt
      721 atgtacggtc acgcggaaat caaaatgtac cctggcgaac caggtagcgg ctttgtgttt
      781 gaaaacaaag tggttggtgg gtcaatcccc cgcgaataca tcaaaccggt tgaaaacggc
      841 attatcgaag cgatgggtcg cggtatcgtg gctggcttcg aaatggttga tattcgggtt
      901 gaactggttt tcgggagcta ccacgaagtt gactcaaacg aaatggcgtt caaaattgct
      961 ggttcaatgg ccttccagga tgctgcgaag aaagccaagc cggttcttct ggagccgatt
     1021 atgtcagtcg aagtcgaagc cccagaagaa tatatgggcg caattacggg tgacctgagc
     1081 tcacggcgtg gccggattga aaaaatggat gaacggccag gaacgaagat cattaccgcc
     1141 gccgtgccgc tggcaactat gtttggatac tcgaccgatc tgcgttcagc gactcaagga
     1201 cgcgccatct acaccatgca cttcaagtgt tacgaagaag caccgaagtc agtgagcgat
     1261 gaaatcatcg cccgggtgaa ggggacggct gcctaactca agttgaataa agaatgtgga
     1321 gcgatggaaa acagattcta acgctccacc ttcatcctca tatttcaaaa aattttcatt
     1381 cgtcggagca acagacaatg gcaaaagaga aatttgatcg cagtaagcca catatcaatg
     1441 tgggaacaat tg
//
LOCUS       DAOQDO010001947         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_103900_length_1449_cov_2.587518, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001947 DAOQDO010000000
VERSION     DAOQDO010001947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103900_length_1449_cov_2.587518"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(539..>1449)
                     /gene="hslO"
                     /locus_tag="PKZ53_24015"
     CDS             complement(539..>1449)
                     /gene="hslO"
                     /locus_tag="PKZ53_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNJ43568.1"
                     /translation="LSTSSQPDFLLYALAASDTVRCVTAVTTNLVQEACNRHLTWKTA
                     SVALGRALTGGALIASQLKELERLTLQFQGRGPLGSITVDADAHGRVRGFIQNPQTDM
                     PLRADGKFDVGGAVGKGVLHVMRDAGFEIGFMKDPYRGSVPLVTGEIAEDLTFYLTNS
                     EQIPSAMGLGVYMDRGSGHVRAAGGFLVQVLPGTSDEVISHLEQAIIEAPPSTELVSA
                     GATPEDIMKVVLGGIDYRILAEKPLKFECTCSYERALSIISALGDVEVRDMLEKDGQA
                     EMTCHYCNAMYHLDAETLARILSGEI"
BASE COUNT          376 a          371 c          362 g          340 t
ORIGIN      
        1 ataagaaatc ttgctaagta acctctggct acggctgaat gtaataccag tttgcagtca
       61 gtagtcagtc attagcagtc catcaagtct attttactca atcgtttgac tgtatcctat
      121 ggattgcaaa ttggtataat gaaatgagcc ggctctgaaa aaggtaaaaa cctgtcagaa
      181 cgttgaaggc cattacggtt gaggtttttc cttagccggc cttactctta aacgcgaaga
      241 atccagcaaa aaatgctcat tcgtcatccg gcacccccgc aaagataggc gcgcactgaa
      301 ccaggccagc ggctcaaaaa agaaaacccc gagcaaatcg gggtttttat accattgagg
      361 gttcagggtt cagggttcgg ggttcagggc tcagggttca gggaaataca attttctcaa
      421 tcgtttagcc tgcccgaaat acatagattg aatttgggaa gtatttgatt cttttcgtgt
      481 atttggtgtg tttcgtggtt aaaaatatcc gggaattttc ggtaagctac ggaacgggtc
      541 aaatctcgcc gctcagaata cgggccagtg tttccgcatc aagatgatac atagcgttgc
      601 aatagtggca ggtcatttca gcctgcccgt ctttttcgag catgtcgcgg acttcgacgt
      661 ctcccagggc tgaaataatg gacagggcgc gctcgtacga gcaggtacat tcaaatttga
      721 gcggtttttc agccaggatc cggtaatcaa tcccgccgag gacaactttc ataatgtctt
      781 cgggtgtggc accagcgctg accagttcag tactgggtgg cgcctcgata atggcctgct
      841 ccagatgcga aatcacctca tcactggttc caggcagaac ctgaaccaga aatccacctg
      901 cggctcgaac gtgcccgctc ccgcgatcca tgtagacccc aagccccatc gcggacggaa
      961 tttgttcaga gttggtcagg taaaaggtca ggtcttcggc aatctcacca gtaaccagcg
     1021 gcaccgatcc gcgataggga tctttcataa aaccgatttc aaatcccgcg tcccgcatca
     1081 cgtgcaacac gccttttcca accgcaccgc caacatcaaa cttgccatct gctcgtagcg
     1141 gcatatccgt ttggggattt tggataaatc cacgaacccg gccatgggca tcagcatcaa
     1201 ccgtaattga cccaagtgga ccacgacctt gaaactggag ggtcagacgc tcaagttcct
     1261 taagctggct ggcaatcagc gcgccaccag tcagggcccg gcccagcgcg acagaagcgg
     1321 tcttccaggt caaatgacgg ttacaggctt cctgcaccag attggtggta acagcggtca
     1381 cgcaacgaac ggtatcgctg gcagccaggg catacagcaa aaaatcaggt tgagatgagg
     1441 ttgaaagta
//
LOCUS       DAOQDO010001948         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104003_length_1448_cov_2.898780, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001948 DAOQDO010000000
VERSION     DAOQDO010001948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104003_length_1448_cov_2.898780"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1448)
                     /locus_tag="PKZ53_24020"
     CDS             complement(<1..>1448)
                     /locus_tag="PKZ53_24020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ43569.1"
                     /translation="GMLLISELAPIMIPVGLGYTIIKHRVFNIQVVIRRGVQYLLAKN
                     ALRALISLPLIALVVTILVNPNRTVREILIQNRLLVTVSILAGAGLIFRQQVTKWIDR
                     RFFREEYNQEQIMLRLIQEMKTLNSFSEISKLVNKEVEAALHPKSMYVFEKEQHLQAQ
                     TLVYSTHRLPQQLSIPDNFQLLRTIELEPQILEAPFTLKKPLPPHELEWLEELGVNLM
                     IPMCGTDHRLIGLLLLGEKKSEEPYTPTDQKLLLVIAEQMAVVYENTRLKERVGREQT
                     LRKQVLERLEEQNINIVKECPTCGICYDSTDEYCSKDKTELTLSLPVERVIERKYRLE
                     QLLGKGGMGAVYEATDLRLNRRVAIKIILSSMFGNPAALRRFEREARAVAMLNHPNVI
                     AVYDYGRTSTDGAFLVMELITGISLREATRRLGGVLDPETAANWFEQILSGIQAAHES
                     GIVHRDLKPENILLIEQANGQKIVKILDFGLA"
BASE COUNT          358 a          382 c          324 g          384 t
ORIGIN      
        1 tcgctaaccc gaaatcaagg attttcacaa tcttctggcc gttggcctgc tcaataagca
       61 ggatattttc aggctttaga tcccggtgaa caatcccaga ttcgtgcgct gcctgaatac
      121 cgctcaagat ttgctcaaac cagttggctg ccgtttccgg atccagaaca cctcccaatc
      181 ggcgggttgc ttcacgcaga gaaatacctg taatgagttc catcaccaaa aaagcaccgt
      241 ccgtgctggt tcgaccataa tcgtagacgg caatcacatt gggatggttt aacatcgcca
      301 ctgcacgggc ttcccgctca aatcgtcgga gggctgccgg gttcccaaac atactgctca
      361 aaatgatttt aatggcgacg cgtcggttca gccgtaaatc agtggcttca tacacggccc
      421 ccatgccacc ctttccaagc aattgctcca accggtattt tcgctcaatc acgcgctcga
      481 ccggcaacga caacgttagt tcggtcttat ctttcgagca atattcatcg gtactgtcat
      541 aacaaatgcc acaggtggga cattctttga caatattgat gttttgttct tccagacgtt
      601 ccagcacctg cttgcgcagc gtttgttccc ggccaacccg ctcctttaac cgggtatttt
      661 catacaccac ggccatttgt tcagcaatca ccagtaacaa tttttgatcg gttggggtgt
      721 agggttcttc tgatttcttt tctccaagta acaacaaccc aattaaacgg tgatccgtcc
      781 cgcacatggg gatcattaaa ttgacgccaa gttcttcaag ccactccagt tcgtgaggag
      841 ggagcggttt tttgagcgta aatggcgctt ccaggatttg cggctccagc tctatggttc
      901 tcaataactg aaaattgtct gggattgaaa gctgctgagg aagccgatgg gtcgaataca
      961 ccagcgtctg agcctgcaga tgctgttctt tctcaaaaac atacattgat ttcgggtgca
     1021 gcgccgcctc gacttcctta ttcaccagtt ttgaaatctc agagaatgaa ttcaaggtct
     1081 tcatttcctg aatcagccgc aacatgatct gttcctggtt gtactcttcg cggaaaaatc
     1141 tccgatcaat ccatttggtt acctgctgac ggaaaattaa gcccgctccg gccaggattg
     1201 aaactgtcac cagaagccga ttttgaatca ggatttccct caccgtacgg ttggggttga
     1261 ccaggattgt gacgaccaga gcaatcaagg ggagtgaaat caatgcccgt aaggcgtttt
     1321 ttgccagcag gtactgtacc ccacggcgga tcactacctg gatattaaat acccggtgtt
     1381 taatgattgt atatccaagc ccaaccggaa tcatgatcgg ggccagttcc gaaatcaaca
     1441 acatgcca
//
LOCUS       DAOQDO010001949         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104112_length_1448_cov_1.613783, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001949 DAOQDO010000000
VERSION     DAOQDO010001949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104112_length_1448_cov_1.613783"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PKZ53_24025"
     CDS             <1..917
                     /locus_tag="PKZ53_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43570.1"
                     /translation="ARRGFFGVQFRITNDGANGEEVYLRQHKSGLPDAMQYTPVLNTG
                     RNWQLYNGQGFTGAVDIPKDTWFHLRLEVTGAQARLYVTDMDKPALVMNDLKTGVQTG
                     QVALFVLTGATYFSNFEIRKTPDMAWERHLPPMPPDTLTRWELSPAYDGLARNLEQPL
                     LPAESKAMGWQTVEAEPPGLVVIYRYREAPHPVVSFANDFSKRLDPQPGMKVVYARTF
                     IDSDRDQVKKLSIGYSDEVSVFLNGRILFQGRSAQNFRDPGFLGIVNPENDTVYLPLK
                     KGKNELVLAISELGGGWGFICRLTSLKN"
     gene            complement(988..>1448)
                     /locus_tag="PKZ53_24030"
     CDS             complement(988..>1448)
                     /locus_tag="PKZ53_24030"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ43571.1"
                     /translation="GATIGELPVFDGGRYPSTVIADQDSQVLFIDKQDVRRLFLEYPT
                     IALEALAVLSQRLRQCAELIEFLSLREVGQRIARLLVLEARKNGKATGNGVTVTLHLT
                     NQQIAAQVGSVREVVSRAIARLQKEHLVVFQGRSVVIPDLEALAQYADFS"
BASE COUNT          362 a          401 c          375 g          310 t
ORIGIN      
        1 cggcacgccg tgggtttttc ggtgttcagt ttcgaatcac caacgatggc gctaacggcg
       61 aagaggtgta cctgcgccag cacaagtctg gacttcccga cgccatgcag tatacgccgg
      121 ttctcaatac tggtcgtaac tggcaactct ataatggaca aggctttacc ggcgccgttg
      181 atatcccgaa agatacgtgg tttcacctgc gccttgaggt aaccggcgcg caagccaggc
      241 tctatgtcac cgacatggac aagcccgcac tggtgatgaa cgatctgaaa actggtgtcc
      301 aaaccggtca ggttgccctg tttgtgctga cgggcgcgac gtatttttca aattttgaaa
      361 tccgaaagac acctgatatg gcctgggaac gtcacctgcc gccgatgcca ccagacaccc
      421 tgaccagatg ggagctgtca ccggcctatg atgggctggc acgtaattta gaacagccgc
      481 tattgcctgc tgaaagcaag gcgatggggt ggcagacagt ggaggcagag ccgcctggac
      541 ttgtggtcat ctatcggtat cgagaggcac cacatccagt ggtgtcattt gcaaacgact
      601 tttcaaaacg tctcgaccct caacctggga tgaaggttgt gtatgcgcgg accttcattg
      661 attctgaccg cgaccaggta aaaaaattgt ccattggcta tagcgacgag gtcagcgttt
      721 ttctgaatgg cagaatactt ttccagggcc gaagcgccca gaatttccgt gatccaggat
      781 tccttggtat cgtgaacccg gaaaatgaca ccgtctacct cccattaaag aagggcaaaa
      841 atgaactggt cctggcaatc agtgaactcg gtggcggatg ggggttcatc tgccgtttaa
      901 ccagcttgaa aaactgaact tccgcccctg gacagaaaag atcaactcag agtccagcgt
      961 gtcagagagc atcgtccaaa cagcctctca ggaaaaatca gcatactggg ccagtgcttc
     1021 caggtcagga atcacaactg atcgcccctg aaaaacaacc aggtgttcct tttgcaggcg
     1081 ggcaatggcc cgtgagacaa cttcgcgaac cgagccaacc tgcgccgcaa tttgctgatt
     1141 ggtcagatgg agggtcaccg taaccccatt gcccgttgcc ttcccgtttt tgcgggcttc
     1201 cagtaccagg agccgcgcaa tgcgctgacc aacttcacgc agcgacaaaa attcaatcaa
     1261 ctcggcacac tggcgcaatc gttgcgacag caccgccagc gcctcaaggg caatcgtcgg
     1321 atattctaaa aacagccgcc gcacatcctg tttatcaata aacagcacct gtgaatcctg
     1381 gtcggcaatg acggtcgaag gataccgccc gccatcaaaa actggtaatt ccccaatggt
     1441 tgccccag
//
LOCUS       DAOQDO010001950         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104436_length_1445_cov_3.235252, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001950 DAOQDO010000000
VERSION     DAOQDO010001950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104436_length_1445_cov_3.235252"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1087)
                     /locus_tag="PKZ53_24035"
     CDS             complement(287..1087)
                     /locus_tag="PKZ53_24035"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNJ43572.1"
                     /translation="MSRLPIVTTQPDLTRRRTRQLPVIVKRTLTHFDRQSKIWLESVL
                     ESGDLPTNYCGQGCFHCCEFPVQATLLEAQHLAAGLPESIWPVIARRVEHLQRLAGEA
                     RDLSDFDEQVRHRLGTCALLDEARKCLAYSRRPLGCRKTYSTLPGDYCARTAQEQMTP
                     QEWHQYQHWISVNPLTGQLDHYIEPLNDFGSELSEKILEAMERELGFSVEGELTVLLW
                     LTRSAEVMEGFWNGDRSRLQSVLDQLGLAHPFLTLIDAQPSPCSGRGE"
BASE COUNT          383 a          361 c          367 g          334 t
ORIGIN      
        1 tcatagtcgt cattccagtc actggctcgg gctcgtgggg gaactggcag caggattccg
       61 cgcatggtga gaaccgccgg cgaaagaggg atgctgtaac gaagaaactc gcttcctgct
      121 gactctaaat ccttttcaca acgaatgtcc ggccattcca tagcaccctc gcttgatgtg
      181 cgattgttgg tgaaaagctt aaatcaaaaa tcgtgaagcg caaagaaaga actgcctgga
      241 gatgattttg aatgatacga gtttgatatt gaaacgaaaa gacttgtcat tcgcccctac
      301 ctgaacaggg gctgggctga gcgtcaatga gtgtcaaaaa cggatgagcc aatccaagct
      361 gatccaaaac cgactggagg cgtgaccggt caccattcca aaacccttcc ataacctcag
      421 ccgaacgggt caaccacaac aagacggtca gttcaccttc caccgaaaaa ccaagttccc
      481 gttccatggc ctccagaatt ttttcagaga gttcagatcc aaaatcattg agtggttcaa
      541 tgtaatggtc gagctggcct gtcagtgggt tgaccgagat ccagtgctgg tactgatgcc
      601 attcctgggg tgtcatttgt tcctgggccg tacgggcaca atagtcacct ggaagcgtgg
      661 agtaggtttt tcggcatcca agcggtcgcc gtgaataggc aagacatttt ctggcttcat
      721 caagcagggc acaggttcca aggcgatgac gaacctgctc gtcgaaatca ctgaggtcgc
      781 gggcttcacc ggcaagtcgt tgtaagtgtt cgacccgcct ggcgatcacc ggccagattg
      841 attcaggaag cccggctgcc aggtgctggg cttcgagtag tgtggcctga actggaaatt
      901 cacagcagtg aaaacacccc tggccacaat aattggttgg caggtcgccg ctttccagga
      961 ctgattccag ccagattttt gattgccggt caaaatgagt cagcgtccgt ttcactataa
     1021 ccgggagctg ccgggttctt cgtctggtga gatcaggttg agtggtgacg attggcaatc
     1081 gtgacataac cccgacggta agagctgaaa agttcctcga aaccaaaagt gctcacagat
     1141 acatatagaa tttcagataa agaaaccacg gaaaccacgg aacacacaga aaataaatcc
     1201 aaattcttca atcatttctg agacgtccca aaacaaatac tcaggaaata tttatctgaa
     1261 aggctatcgt tgctgaggtg ctggcatcct actggtatca atcggttgct cttcgctcag
     1321 gtgtcagccg gataccagca ccccgaccag gtttctatac ctccgaagcc tgtatctgaa
     1381 gtggccgaat gaccctgacc actggatact ggttcatatc tggctcaaga tagccagagc
     1441 gttca
//
LOCUS       DAOQDO010001951         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104473_length_1445_cov_2.353957, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001951 DAOQDO010000000
VERSION     DAOQDO010001951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104473_length_1445_cov_2.353957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKZ53_24040"
     CDS             complement(<1..275)
                     /locus_tag="PKZ53_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43573.1"
                     /translation="MYNFAFFFEVMDKEPGLIEIWLFYAALAVIGFGLTRFRSWLLAI
                     VFPINLLLGLGWLTELHDQHIGPVILQEAGYAYVIQSYLAIVLAVVLP"
     gene            complement(340..1083)
                     /locus_tag="PKZ53_24045"
     CDS             complement(340..1083)
                     /locus_tag="PKZ53_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43574.1"
                     /translation="MSQDLTSTKHDWSDRWEHIYQFIHAWTGLNFRPQPDIKRLEKLE
                     LELGITLPPSAREWCFLGWSASKLRGYFSFRDGPLFVTSFPEHNAISLLTQAEGDVKW
                     VIETQDLDKDDPPVMAFFLDYEADEETFAKRGMWAPTVSAFAFDYFLAYLDSTGGGFA
                     VSTDSFPYDRAALAADFGEPTQFGDTEYFLRDGILVFTSHNPISWHHEVVKVEFQRSI
                     PVEQLPPSIQVLSKAAYMRSGAFIDLDKF"
     gene            complement(1091..>1445)
                     /locus_tag="PKZ53_24050"
     CDS             complement(1091..>1445)
                     /locus_tag="PKZ53_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43575.1"
                     /translation="TSPSARIERDLQKLRSKGWSEAKIQRWQSQREDSSHNHHHSSES
                     ERWILLIHKLLEQPQAKRIGLFLHWYSASVELEHLIISDTRKVQLSELTVDFLTHLQE
                     DVVYAFVNDQKGWIG"
BASE COUNT          406 a          354 c          335 g          350 t
ORIGIN      
        1 ggaagcacca ccgccagcac gatggccaga taagactgaa taacgtaggc atatccagct
       61 tcctgaagaa taaccggacc aatatgttga tcgtgaagct cggttaacca gcccaatccc
      121 aaaagcaaat tgattggaaa aacaatggcc agcaaccagg aacgaaaccg ggtcaaccca
      181 aaaccaataa ctgccagggc cgcataaaaa agccagattt cgatcaaccc cggctcttta
      241 tccatgactt caaagaagaa ggcaaaattg tacatatgag ttgaaccacc cggtaggaga
      301 ctcaaagctc cctcgatctt tgagtgatgg gatagttgct cagaacttat ccaggtcaat
      361 aaaagcacca cttcgcatat aagcggcttt ggatagcact tgaattgatg gcggtagctg
      421 ctcaaccgga atacttcgtt gaaactccac tttgacaacc tcgtgatgcc aggaaatcgg
      481 gttgtgcgag gtaaacacca ggattccatc gcgaagaaag tattcagtgt caccaaattg
      541 cgtgggttct ccgaaatcag ccgccaatgc cgcccggtca tacggaaagg agtcagtact
      601 cactgcaaat ccaccacctg tgctgtccag atacgccaga aagtagtcaa aggcaaaggc
      661 tgaaacggtt ggcgcccaca ttccccgttt tgcaaaggtt tcttcgtcgg cttcataatc
      721 caggaaaaag gccatcactg gcggatcatc cttgtccaga tcctgagttt caatcaccca
      781 tttcacatcg ccttcagcct gggtcagcaa cgaaatggcg ttgtgttccg ggaatgaagt
      841 cacaaacaat ggtccatccc gaaaagaaaa ataacctctg agttttgagg ctgaccagcc
      901 aagaaagcac cattcgcgag ccgaaggtgg aagggttatt ccaagttcca attcaagttt
      961 ttcgagccgt tttatatccg gctgaggtct gaaattgaga cccgtccagg cgtgaataaa
     1021 ttgatagata tgctcccacc ggtctgacca gtcgtgtttg gtacttgtaa gatcttgtga
     1081 cataagcgtt tcaaccaatc catccttttt gatcattcac aaatgcatac accacatctt
     1141 cttgcaggtg cgtcaggaag tccaccgtca attcgctcaa ttgtactttc cgggtatctg
     1201 aaataataag gtgttccagt tcaaccgaag ccgaatacca gtgaaggaac aaacctatcc
     1261 gctttgcctg tggttgttcc aacagtttgt ggatcaacag gatccagcgc tcagattcag
     1321 acgagtgatg gtggttgtgt gatgagtctt ctctttgtga ttgccagcgt tggattttgg
     1381 cctcagacca ccctttggat cggagttttt gaagatcgcg ttcaattcgg gctgatggtg
     1441 acgtg
//
LOCUS       DAOQDO010001952         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104580_length_1444_cov_3.007199, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001952 DAOQDO010000000
VERSION     DAOQDO010001952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104580_length_1444_cov_3.007199"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKZ53_24055"
     CDS             complement(<1..1117)
                     /locus_tag="PKZ53_24055"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ43576.1"
                     /translation="MKKSSGPHNNSIIGRILYGTLFVGIVPWLLWIWARALHSQFQHW
                     LIPVSPNSGVFIASAGMVVMVLGMWALWVWGEGLPMNAYPPARFVTAGVYRWLPHPIY
                     TGFIVFCFGASAWLNLPAGFWLVSPLVIAGCLALVWGYENPDLVRRFPEARATSWLRL
                     PPNTLESPDFLERVAGFFLTLFPWGLVYVLLALIPSAPDLCEVRGLWEFDVPRPGWAL
                     WPYNFAYGWVVLALLIVPSRQKLRDFVLDGWVLSLTGYVVMLGLPFAAAALPIVGTPF
                     EQALWVANCECDASWLAVPSFHVAWSVIACTPFADRWPRLRWVWWFIAASISISCVLV
                     GLHGSLDVVTGVALAGLAHHRHIVWGKLLTATEYLGNS"
     gene            complement(1089..>1444)
                     /locus_tag="PKZ53_24060"
     CDS             complement(1089..>1444)
                     /locus_tag="PKZ53_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43577.1"
                     /translation="WGRWIGSQHTLTWVEWEGRHPRRVAILNGHCCSLVNIGPTRVFL
                     ENGCHLAFGTPKVLVHARLGEDALRIFSYLPGLIPRPFLDGVECKYLAPATLTCGNSQ
                     DEGWAIYEEVIWPAQ"
BASE COUNT          421 a          403 c          368 g          252 t
ORIGIN      
        1 agctattgcc aaggtattcg gttgctgtca gaagttttcc ccaaacaatg tgccggtggt
       61 gagccagtcc agccagagcg actccggtga cgacatccag gcttccgtgc agtccaacca
      121 ggacacaact gatggaaata ctggctgcta taaaccacca gacccaccga agacgcggcc
      181 agcggtcagc aaatggagtg caggcaatga ccgaccaggc aacatgaaaa gagggaactg
      241 ccagccacga ggcatcacat tcacaattgg cgacccacag tgcctgttca aacggggttc
      301 cgacgattgg aagtgctgct gccgcaaagg gcaatccaag cataacaaca tagccggtaa
      361 ggctcaaaac ccatccatcc agaacaaaat cacgcagctt ctggcgtgag ggaacaatga
      421 gcaaggccag tacaacccat ccataagcaa aattgtaagg ccaaagcgcc catccaggcc
      481 gtggtacatc aaattcccag agtccacgaa cttcacacag gtccggtgcg gatggaatca
      541 aagccagcag cacatagacc aatccccacg gaaacagggt cagaaagaac ccggcgactc
      601 gctctaaaaa gtcaggtgat tccagcgtgt tgggcggcaa ccggagccaa ctggtcgcac
      661 gtgcctctgg aaaccggcgt accagatcgg gattttcata tccccagacc agtgccaggc
      721 atccggcgat gaccagtggt gaaaccagcc agaatccagc cggcaggttg agccaggctg
      781 aagcgccaaa acaaaacaca ataaaaccgg tataaattgg atggggtaac cagcggtaaa
      841 cccccgccgt gacaaaacgt gctggagggt aggcattcat gggcaatcct tcgccccaaa
      901 cccacagggc ccacattcca agcaccatga cgaccattcc agcactggca ataaaaaccc
      961 ctgaatttgg tgaaacagga atcagccagt gttggaactg tgagtggaga gccctggccc
     1021 atatccagag tagccaggga acgataccaa cgaaaagggt tccgtataaa atacgtccga
     1081 taatggaatt attgtgcggg ccagatgact tcttcataaa ttgcccatcc ttcatcctgg
     1141 gaattcccac aggttaacgt ggcgggcgcc aggtatttac actcgacgcc atcaaggaag
     1201 ggacgtggga ttaaccctgg aaggtaagaa aagatgcgca acgcatcttc cccaagtctg
     1261 gcatgaacca gaactttggg tgttccaaaa gccagatggc atccattctc caggaaaacc
     1321 ctggttggac caatgttgac cagtgagcaa cagtgtccgt ttaatattgc aacccgccga
     1381 gggtgacggc cttcccattc aacccaggtc aaggtgtgtt ggcttccaat ccagcgaccc
     1441 cagc
//
LOCUS       DAOQDO010001953         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104602_length_1444_cov_2.464363, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001953 DAOQDO010000000
VERSION     DAOQDO010001953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104602_length_1444_cov_2.464363"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PKZ53_24065"
     CDS             <1..1394
                     /locus_tag="PKZ53_24065"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43578.1"
                     /translation="PVAAQAEARKSVDALVLQYETLLSKIREQSPRYAALTQPEPLTL
                     EAVQKTVVEPGSLLLEYSLGKDQSFVWAISPTQVFWYELPGQERIEPAVSKVRDLLLT
                     RNQLPAHLLRDQRGDRIKQAEKEFTSAAAELSDLILAPVAAHLGTNRLLIVADGALQY
                     LPFAALPEPGGKGTAAGERTPLVVRHELVSLPSASTLAVLRAEAPTKPKADPGKLVAV
                     FADPVFDVEDPRFQHLMKRDPLVAENPPSNRVEGIELNRLRQLTPVVKPDGKLSIPRL
                     PFTRQEATEIRTLATSARTALFLDFAASHNKVTTADIGKYQYLHFATHGLLDTEHPEF
                     SALVLSLVDEKGNRQKGFLRMMEVYNLDLAAQLVVLSACETGLGKNIRGEGLIGLTRG
                     FMYAGAPSVVVSLWSVSDQGTSELMKRFYRGLLTEKLRPAAALRKAQIELMGQKAWNS
                     PFYWAPFIVQGEY"
BASE COUNT          334 a          404 c          388 g          318 t
ORIGIN      
        1 ccccagttgc agcccaggcc gaagcgcgca aaagcgtgga tgcgctcgtc ctccaatatg
       61 aaacgctcct ttcaaagatt cgggaacaaa gcccgcgcta tgcggcactc actcaacccg
      121 aaccactcac gcttgaggca gtccaaaaaa ctgtggttga gcctggcagc ctcctgcttg
      181 aatactcact tgggaaagac cagagctttg tctgggcaat cagcccgacc caggtctttt
      241 ggtatgaatt accagggcag gaaagaattg aaccggcggt cagcaaggtg cgcgatctct
      301 tgctgactcg aaaccagctt ccagcccatc tgctgcgcga tcagcgtggc gaccggatca
      361 aacaggccga aaaagaattt acttcggctg ccgccgaatt aagtgacttg attctggccc
      421 ctgtggcggc tcatctgggc accaaccggc tgctgattgt ggccgatggg gcattacaat
      481 accttccttt tgccgccttg ccggaaccag gtggaaaagg aacggctgcg ggtgaacgca
      541 ctccgctggt ggttcgccac gaactggtca gtttgccgtc agcctcaacc ctggctgtac
      601 ttcgtgccga agccccgacc aaacccaaag ccgaccctgg caaactggtg gccgtgtttg
      661 ccgatccggt ttttgatgtc gaagacccac ggtttcaaca cctgatgaag cgggatccac
      721 tcgttgccga aaatccgccc tccaatcgag ttgaaggcat cgagttgaat cgcctccgac
      781 aattgacccc ggtggtaaag ccagatggga aactatcaat ccccaggctt cccttcaccc
      841 ggcaggaagc caccgaaatc cgcacgctgg ccacatccgc gaggacggct ttgtttcttg
      901 attttgccgc cagccacaat aaagtcacca cggctgacat tggaaaatac cagtaccttc
      961 actttgccac tcacggtctg ctcgataccg agcatcctga gttttccgcc ctggttctgt
     1021 cactggtgga tgaaaaaggc aaccgtcaaa aagggttttt gcggatgatg gaagtctata
     1081 accttgacct ggcggcccaa ctggttgtgc tttcggcctg tgaaaccgga cttggaaaaa
     1141 atattcgcgg cgaaggtctg attggtctga ctcgtgggtt tatgtatgcc ggtgccccat
     1201 ccgttgttgt cagcctgtgg agtgtgagcg atcagggcac ctcggaactg atgaagcgtt
     1261 tttatcgcgg attgctgact gaaaaactcc gtccggcagc ggcgctccgc aaagcccaaa
     1321 ttgagttaat gggccagaaa gcctggaatt ccccgtttta ctgggcacca tttattgtcc
     1381 agggagaata ttgaagaacc aggactgaaa ggcatcgggc tcagggcttg gggctgaaga
     1441 atat
//
LOCUS       DAOQDO010001954         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104708_length_1443_cov_3.431556, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001954 DAOQDO010000000
VERSION     DAOQDO010001954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104708_length_1443_cov_3.431556"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKZ53_24070"
     CDS             <1..894
                     /locus_tag="PKZ53_24070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43579.1"
                     /translation="PPLAQVYFLAAYRLAGNTLWGIKGLLFLAECGTFWLLFKLLAGN
                     SASANPLWIVVYAWCPLPILECGIAGHVDGLGILFVVLFYLSIQQKQIPLAAVALAGA
                     VLIKLVPVILLLWVWFHLPKADRWRFGLIFGTLIVTGYAPFIEFSREVSVIQSLLVYL
                     GTWSFNGPLYGLGKLLIGGPVSRTLCALGLLYWIWKVSTRVGHFQTAMLGLWFGFFLL
                     TPTVYPWYLLWLAITLSWRWSTSGWWWLVSCPVSYLVLVTRLETGIWHESWLAYVIQY
                     VPLMIFGWREWQSGFRKFSEK"
     gene            complement(1035..1340)
                     /gene="rpsS"
                     /locus_tag="PKZ53_24075"
     CDS             complement(1035..1340)
                     /gene="rpsS"
                     /locus_tag="PKZ53_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNJ43580.1"
                     /translation="MSRSLKKGPFIDPKLAKKLAALGKEDRTIVKTWARSCSIPPEFV
                     GRTIAVHNGRVHVPVFVTENMVGHKLGEFSPTRKFRKHGVTRFQTVLGFLLGNTIWV"
BASE COUNT          297 a          330 c          384 g          432 t
ORIGIN      
        1 ccgccgctgg ctcaggtata ttttctggct gcttaccgat tggccggaaa taccctctgg
       61 gggatcaaag gtctcttgtt tctggctgaa tgcggcacgt tctggttgct tttcaaatta
      121 ctcgccggga attcagcctc agccaatccg ctctggattg ttgtctatgc gtggtgtccg
      181 ctgccgattt tggaatgcgg catagctggg cacgtggatg ggctcggaat tttgttcgta
      241 gtgctttttt atctttcaat ccagcaaaaa cagattccgc tggcggcagt ggccctggct
      301 ggtgccgtac tgatcaaact tgtgccggtg attttgctgc tgtgggtgtg gttccacttg
      361 cccaaagctg accgctggcg atttgggctg atttttggaa cgctgattgt caccggatac
      421 gcaccgttta ttgaattctc acgtgaagtc tcagttatac aatcacttct ggtgtatctg
      481 ggaacgtggt catttaacgg cccgctgtac ggattgggca aattattgat tggtggtccg
      541 gtttcacgca cattgtgcgc ccttgggtta ctctactgga tttggaaggt ttcaacccga
      601 gttggacact ttcagaccgc aatgcttggg ttgtggtttg gttttttcct gctcacacca
      661 accgtctatc cctggtattt gctctggctg gcaatcacgt tgagttggcg atggtcaaca
      721 tcgggctggt ggtggctggt gtcgtgcccg gtgagctatc tggtgctggt aactcgtttg
      781 gaaactggaa tctggcacga atcgtggctg gcctatgtga tccaatatgt gccgttgatg
      841 atatttggct ggcgcgagtg gcaatcaggg tttcggaaat tttctgaaaa ataattttca
      901 acgttttggt gctttgttcg atataccaca atgaacacca aaaaacatat cgggcttggg
      961 gctgagggct gagggctgag ggttgagggt tgaaaaaacc agggcttcag ccactagccc
     1021 ggaacccgat accctcagac ccaaatggtg ttacccagta aaaatcctaa aacagtttgg
     1081 aaacgagtga cgccatgctt tcggaactta cgagttggag aaaactctcc aagcttatgt
     1141 ccgaccatgt tttctgtcac gaagacagga acatggacgc ggccgttatg gactgcaata
     1201 gttcgaccaa caaattctgg tggaatcgaa caagagcgtg cccaagtttt tacgattgtg
     1261 cggtcttctt taccaagcgc tgctagtttt ttcgccagct ttggatcgat aaatggacct
     1321 tttttcagtg atctgctcat cctattacct tatttcctct tagcgtcgtg gcgactgcgc
     1381 acgataaatt tagttgttgt cttacgacgt cgtgtcttat aacccagcgt cttctggccc
     1441 cat
//
LOCUS       DAOQDO010001955         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104803_length_1443_cov_1.830692, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001955 DAOQDO010000000
VERSION     DAOQDO010001955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104803_length_1443_cov_1.830692"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..523)
                     /locus_tag="PKZ53_24080"
     CDS             complement(74..523)
                     /locus_tag="PKZ53_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNJ43581.1"
                     /translation="MIDEIDSKILTILQKEARTSNAEIARQLGMAPSGILERIRKLEE
                     RGVIQGYRAEINPKALGLGLLAFVAVRSNDRVNDIATGKLLAQIPGVLEVHDTAGEDC
                     YLVKIRAADTESLGKIIRTQIGTIPSVLTTRTTIVLETLKESTEMPI"
     gene            806..1369
                     /locus_tag="PKZ53_24085"
     CDS             806..1369
                     /locus_tag="PKZ53_24085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43582.1"
                     /translation="MKYLMVRLSLILLLILGHLAGSLVSASPHVSENQTPAPAPVSPS
                     NEAITPGIPIEKPIRGGEKHRYSLTLSQGDFVRLVIESTTLTPAIEITTDDGKTMGAS
                     KIADRVVQRTIVFLIAETAGTYHVTVYPAREGCPPGRYQISVPALRPATDEDRERVAA
                     QTEFFATGELLAKRTKEALDEGCQRCQ"
BASE COUNT          353 a          404 c          333 g          353 t
ORIGIN      
        1 aacgccacaa ccagtttcca ccgagctacc ttcgtaaaga gttgcatgcc gataaacctc
       61 cttcacatgc aggtcagatc ggcatttcag ttgactcctt gagggtttcc aaaacgatgg
      121 tggtgcgcgt ggtgagcacc gaaggaatcg tgccgatctg ggttcgaata atcttgccta
      181 acgattcagt atccgcggcc cggattttga ccagataaca gtcttctccg gcagtatcgt
      241 gaacttcgag cacccctgga atctgggcga gcaacttgcc agtggcaatg tcgttgaccc
      301 ggtcgttgct gcgcaccgcg acaaaagcca gcagacccaa acccaacgcc ttcgggttga
      361 tttcagcgcg atagccctgg atcaccccgc gttcttcgag tttgcgaatc cgctctaaaa
      421 tccctgatgg cgccatgccg agttgacggg caatctcagc atttgaagtt cgcgcttctt
      481 tttgaagaat cgtcagtatt ttactatcaa tctcatcaat catacttaaa aacaggtata
      541 ttaaagaaca ttcatctcat aaaacgatat tttagagaat aaagttctct tgtcaaccct
      601 tcctttctga aaaatggctg gctggcaatt gcggtgggaa ccttttcgaa ttggaaatat
      661 ccaggcacaa tagctgaaga aaccctcgtc tgacactgta atttccgctc ctccccaaac
      721 gctcatattc cttcaatctc atcttctttt tctggcagcc ccccagcttt cacccccttt
      781 gtttttccgg taggagacac tgtgtatgaa ataccttatg gtgcgcctga gcctgatact
      841 actactgata ttgggacacc tggctggcag tcttgtgagt gcctcacccc acgtttctga
      901 aaaccaaaca ccagcgccag caccggtcag tccttcaaac gaagccatca cgcccggaat
      961 cccgattgaa aaaccgatcc ggggtggtga aaagcaccgc tattccctca cattgagcca
     1021 gggcgatttt gttcgactgg tgattgaatc aaccactctc actccggcta ttgaaatcac
     1081 caccgatgac gggaaaacga tgggtgcttc caaaattgcc gaccgggttg tacaacgtac
     1141 cattgtcttt ttgattgcgg aaaccgctgg aacctatcac gttacggtct atcctgcccg
     1201 cgagggctgc ccaccgggcc gatatcagat ttccgttcca gccctccggc ctgccacaga
     1261 tgaagaccgc gaacgggtgg cagcacaaac ggaatttttt gccactggcg agttgctcgc
     1321 caaacgaacc aaagaagcac ttgacgaagg ttgtcagcga tgtcaataga ctcgaccctc
     1381 aagggccaag cttgagtcgt gtacccacga cgccatcctg acggatggtc ggtcgtgggt
     1441 gga
//
LOCUS       DAOQDO010001956         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104861_length_1442_cov_3.718097, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001956 DAOQDO010000000
VERSION     DAOQDO010001956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104861_length_1442_cov_3.718097"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..952)
                     /locus_tag="PKZ53_24090"
     CDS             complement(152..952)
                     /locus_tag="PKZ53_24090"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNJ43583.1"
                     /translation="MAHKRQIITLMTDFGTSDHFVAAVKASILNIHPDVDIIDITHDI
                     PAHDVFAGAFTLRNAISLFPRFTLHMAVVDPGVGSARRPIVVMTDNFNFIGPDNGLFS
                     YIYQEETVNRVLHINAEHYFRHPVSPTFHARDIFAPIAGYIAKGVDPRKVGDEITDFV
                     RFDTPKPDASNPKMVRGAILHVDRFGNCITNLTRNEITLSAIRAGGKLVINNREVVKF
                     CTHYAEAQPNELFMLFSSTNLLEVAALKTPAARVIEARRGMEVYFVVP"
     gene            1135..>1442
                     /locus_tag="PKZ53_24095"
     CDS             1135..>1442
                     /locus_tag="PKZ53_24095"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ43584.1"
                     /translation="MIYTYDLSTGVSLRVNLSGEGEPIVFIHGLGGNFSFWAGQVRDL
                     AYRFQVIRYDLRGHGGSSVSSVQTPQDHVADLAALLETLEIDQVRLVGLSMGGVIAQT
                     "
BASE COUNT          362 a          324 c          403 g          353 t
ORIGIN      
        1 gacagaaagg gcgacaagga cgaaaaagac cgggtggaag tcggtggtgc ggaagtgaca
       61 gggaaaaggc caggctattt ggggcagaat tgaagccatt ctcaattctt cattcttcat
      121 tcttcattct caattcttca ttctcaacgg atcagggcac aacgaaataa acttccatgc
      181 cgcggcgggc ttcaatcacg cgggccgccg gggtttttag tgctgccact tcaagcaaat
      241 tggtgcttga aaagagcata aagagttcat tgggctgggc ttcggcatag tgagtacaaa
      301 atttgaccac ttcgcggttg ttaataacca gttttccgcc agcacggatc gcagaaaggg
      361 tgatttcatt gcgggtgagg ttggtaatgc agttgccaaa ccggtcaacg tgcaagattg
      421 caccccgaac catctttgga ttgctggcgt caggcttggg cgtgtcaaac cggacaaaat
      481 cggtgatttc gtctccgact ttgcgcgggt caacaccttt ggcaatgtat ccagcaatcg
      541 gcgcgaaaat atccctggcg tggaacgtcg gactgactgg atgccggaaa taatgttcgg
      601 cgttgatatg gagcacccgg ttgacggtct cttcctggta aatgtaacta aataaaccat
      661 tatccgggcc aataaaattg aagttgtcgg tcatcaccac aatgggccgc cgggccgagc
      721 caacacctgg gtcaaccacg gccatatgca gcgtgaagcg cggaaagagc gaaatcgcat
      781 ttcgaagggt aaacgccccg gcaaaaacat catgggcggg gatatcgtgg gtgatgtcaa
      841 taatgtccac atctgggtga atattcaaaa ttgaggcttt tacagcggcg acaaaatgat
      901 cactggtgcc aaagtccgtc atcaaggtaa taatttggcg tttgtgggcc atggacagtt
      961 ccccctgaaa tggtgatgat caattgaact ggagagagaa gaagctggaa acgtttgaaa
     1021 aatgggtatg aggtgcgtta cccatgactt ctgtgaggcc gttactctag cataggtgat
     1081 tttcagtgcc aaacagggtt ttttcagcca catgagcatg aaggagcaaa ccacatgatt
     1141 tacacatatg atctttcgac cggagtgagt ctccgagtca atctgtcggg tgaaggcgaa
     1201 ccgattgtat ttatccacgg tctgggcggg aacttttcat tttgggccgg gcaggttcgt
     1261 gatctcgcct atcggtttca ggtgattcgc tatgatctgc gtggtcacgg cggatcatcg
     1321 gtttcaagtg tccagacgcc tcaggatcat gtggcggatc tggctgcgct ccttgagacc
     1381 ctcgaaattg atcaggttcg actcgttggg ctctcaatgg gcggcgtgat tgcccaaacc
     1441 tt
//
LOCUS       DAOQDO010001957         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_104888_length_1442_cov_2.836337, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001957 DAOQDO010000000
VERSION     DAOQDO010001957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104888_length_1442_cov_2.836337"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PKZ53_24100"
     CDS             <1..1149
                     /locus_tag="PKZ53_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ43585.1"
                     /translation="GGFLSGRLSIPVPATRRLVNGKHLSVMGATHNNLKKIDVRFPIG
                     VFTVVTGVSGSGKSSLVGDILYRGLAKQIYGSFSVPGGHTTIEGIEHIDKVIEIDQSP
                     IGRTPRSNPATYTGLFTMIRELYAGLPEARERGYKVGRFSFNVKGGRCEACQGDGLKR
                     IEMNFLPDVYVQCDVCQGARYNRDTLAVRYRGLSIADMLNTTISDALELLSAIPSIKQ
                     KLQTLNEVGLGYLKLGQSATMLSGGEAQRMKLAKELSRRATGRTLYILDEPTTGLHFE
                     DVRKLLYVLHRLVDNGNTVIVIEHNLDVIKSADWLIDLGPEGGVMGGRLVGEGRPEEI
                     ARLKKSHTGQALRAVLGRPTPGGAKGRSRSISPEHWDDEGIELVQDEG"
BASE COUNT          332 a          310 c          446 g          354 t
ORIGIN      
        1 ggtgggtttc tttcggggcg gctctcaatt ccggtgccgg ctacccggcg gctggtcaat
       61 ggcaagcatc tttcggtgat gggggcgacc cataacaatt tgaagaaaat cgacgtccgg
      121 ttcccgatag gggtgtttac ggtggtcaca ggagtttcag ggtcggggaa atcaagtctg
      181 gtgggtgata ttttatatcg ggggctggcc aaacaaattt atggaagttt ttcagttccg
      241 ggtggtcaca caaccatcga gggaattgaa catattgata aggtgattga aattgaccag
      301 tcgcccattg gccgaacacc caggtcaaac cccgccacct ataccggttt gtttacgatg
      361 attcgcgaac tctatgctgg tcttcccgaa gccagggagc gtgggtataa agttggtcgc
      421 ttctcattca acgtcaaagg tggtcggtgt gaagcatgtc agggagatgg tctcaaacgg
      481 attgagatga actttctgcc ggatgtctat gtgcagtgtg atgtctgtca gggcgcacgc
      541 tataaccgtg acacgctggc cgtcaggtat cgagggttgt caattgccga tatgctcaat
      601 accacgattt cagatgccct ggagcttctg agtgccattc cttcaatcaa gcaaaaactg
      661 caaaccctga atgaggttgg attaggctat ctcaagctcg gtcaatcggc caccatgttg
      721 tctggcgggg aagcgcaacg catgaaactg gccaaagagc tttcccggcg cgccactggc
      781 cgaaccctct acattctgga cgagccaact accggattac attttgagga tgtgcgtaag
      841 ttgttatatg tattacatcg cctggtggat aatggtaata ctgtaatcgt aattgagcat
      901 aatctggacg taattaagtc ggctgattgg ctgattgatc ttggaccaga aggtggggtc
      961 atgggcgggc ggctggttgg agagggccgg ccagaggaga ttgcccgatt aaagaaatca
     1021 cataccgggc aggcgctccg agctgttctg gggcgtccga caccgggcgg cgccaaagga
     1081 agatcgcgct cgatttcccc tgagcattgg gacgatgaag ggattgaact ggtccaggac
     1141 gaggggtagt gccaggggga ttgggccggc attcaggttc tttggcgttt gacagtgagg
     1201 atttcaccgg gagattgata taaaaaaaag cctgcagaaa aaattctgca ggcttgagag
     1261 agtagaacag gaagccctca atgggcctac tgggaggagg aactatcagt tggaggcggg
     1321 tctgaatttt gtggtttgcg tttgagcgtt ggccgatcag atggttgagg cggctgcgat
     1381 tgattgtcta tatctccggt ggcatctgga tcaaccggcg cattcggcgc attggggtcg
     1441 gt
//
LOCUS       DAOQDO010001958         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105210_length_1440_cov_2.509025, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001958 DAOQDO010000000
VERSION     DAOQDO010001958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105210_length_1440_cov_2.509025"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKZ53_24105"
     CDS             <1..270
                     /locus_tag="PKZ53_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ43586.1"
                     /translation="VAVIINRALESGSLFQRVKGKSLPPWASELDCASWGQFFLKFVI
                     SHPAVTCVISGTSKPQHMKDNLGAAFGKLPDAPTRRKMVELMSSL"
     gene            391..1200
                     /locus_tag="PKZ53_24110"
     CDS             391..1200
                     /locus_tag="PKZ53_24110"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNJ43587.1"
                     /translation="MQRLNGKIALVTGAAQGIGEAIAERFAQEGAFVVVTDIQDELGA
                     SVAQRYPGQTIYRRLDVRNEADWIAVTDDILRQKGKLDIVVNNAGVTGFHEDLGPQDP
                     EHASLESWRKVHSTNLEGVFLGCKHGIRAMRPARAGVIINISSRSGQVGIPGAAAYAS
                     SKGAVRNHSKTVALYCAEQGLNIRCNSVHPAAILTPMWDHLLGHDDQREARVAMFTSD
                     IPLRRFGLPREVADACLFLASDEAAYVTGIEFVIDGGILAGASAAPGKKDE"
BASE COUNT          347 a          363 c          390 g          340 t
ORIGIN      
        1 gtggcggtta tcatcaaccg ggcgcttgaa agtgggtctt tgtttcagag ggtcaaggga
       61 aagtcactac ctccgtgggc cagtgaactt gattgtgcat cgtggggaca attctttttg
      121 aaatttgtta tttctcaccc ggcggtaacc tgtgttatat caggaacatc caaaccacag
      181 catatgaaag acaaccttgg ggccgcgttt ggaaaacttc cagatgcccc aacccgacgc
      241 aagatggttg agttgatgtc gagcctgtaa tcaaaaccga aacaaattcc caggattcag
      301 tcgttttgtg cgggttcttt tgcggatgaa cgctgaggca ggtacgccct ggtggaatca
      361 cacactgaaa gagaacagaa aggaacgtgt atgcaacgac tgaatggaaa aattgccctg
      421 gttacaggtg cagcccaggg tatcggtgaa gccattgccg aacgctttgc ccaggaaggc
      481 gcctttgtgg ttgtgactga tattcaggac gaactcgggg cctcggttgc gcaacgctat
      541 ccgggccaaa caatctaccg acggctcgat gtgcgcaatg aagccgactg gatcgctgtc
      601 actgacgaca tcctcaggca aaaaggaaaa ctggatattg tggtcaataa tgccggggtg
      661 actgggtttc acgaagacct cggccctcaa gacccggaac atgcctcgct cgaaagctgg
      721 cggaaagtcc actccaccaa tctggaaggg gtttttctgg gttgcaaaca tgggattcga
      781 gccatgcggc ctgccagagc gggggtcatc atcaatattt cctcccgctc tggacaggta
      841 ggcattcctg gtgctgccgc ctatgcctca agcaaaggcg cggtgcgcaa tcattctaaa
      901 accgtagcgc tctattgtgc cgaacagggg ctcaatattc gctgcaattc ggttcacccg
      961 gcggccatcc tgacaccgat gtgggaccac ctgctcgggc acgacgatca gcgcgaagcc
     1021 cgtgttgcaa tgtttaccag cgacattcca ctgcgtcggt ttgggctccc ccgagaagtg
     1081 gccgatgcct gtctgtttct ggcgtcagat gaagccgcct acgtcaccgg catcgagttt
     1141 gtcattgacg gtgggattct ggccggagca tcagcagcac ctgggaagaa ggatgagtaa
     1201 cccttttcgg gttccgggtt ccgctttccg aaagagaagg tgtgctcatt tgcgacaggt
     1261 ttgtcccaaa atcgaacaac cttttaaaaa attttggagt gcggtgacgt ctcaccgctt
     1321 tgattttgag caacttatcg ctggattaca ccattttgga atgaaagcct cgtattatgg
     1381 aaagattaac tcattgaaaa aactgtattt ccctgaaccc tgaacccgat accctgaacc
//
LOCUS       DAOQDO010001959         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105312_length_1439_cov_3.539740, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001959 DAOQDO010000000
VERSION     DAOQDO010001959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105312_length_1439_cov_3.539740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKZ53_24115"
     CDS             complement(<1..660)
                     /locus_tag="PKZ53_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43588.1"
                     /translation="MLYRVRWVGIVIICLAVGILPYVKPVPGRTDTQGVATSGRLNAS
                     PAFPNLTGEKAIEHLKQDGSYDSLAEAMAAARYSANWVTNPRLPHLNGAYEADNPAQG
                     FTSYFTPDGVHLVAAGEGTPAWFLEMKLQGIGYGDKLGTVSVRTPTVTSHRVELARNS
                     SFSILNSSFTEWYENSPAGLEQGLTLDAPPGERNGADRLRVAFDVDGTLEARRTADGQ
                     VI"
     gene            complement(1062..>1439)
                     /locus_tag="PKZ53_24120"
     CDS             complement(1062..>1439)
                     /locus_tag="PKZ53_24120"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43589.1"
                     /translation="QGVAVDGSGNLLIADTGNSRVLKFVGGNGETGVLLSQSGSALGQ
                     VRNPEGVAFSVFSTGPLAGRLMLSVGDTGNNRIVGLDLGDANNQSSTAWPALSSSGFW
                     QLVGAPNRLGSQVGQFRAPSKIN"
BASE COUNT          358 a          391 c          340 g          350 t
ORIGIN      
        1 gatcacctgt ccatcggctg ttcgccgggc ttccagtgtt ccatccacat cgaaggccac
       61 ccgcaaccgg tcggccccgt ttcgttcccc aggcggtgca tcgagcgtca gtccctgttc
      121 taacccggct gggctgtttt cataccactc tgtgaatgaa gaattgagaa ttgagaatga
      181 agaattccgc gccagttcca cccgatggct ggtcacggtt ggtgtgcgaa ctgagacagt
      241 tcctaatttg tcgccatagc caattccctg gagcttcatc tccagaaacc acgctggtgt
      301 accttcccct gccgctacca gatgcacccc gtctggcgtg aagtaactgg taaatccctg
      361 cgcaggattg tccgcctcat aggcaccgtt caggtgcggc aaccgtggat tcgtcaccca
      421 gtttgcactg tagcgggcgg cagccatggc ctccgccagc gagtcgtatg atccatcttg
      481 cttgagatgc tcaattgctt tctccccggt taaatttgga aacgcgggtg aggcattcag
      541 cctgccagaa gtagctacac cttgggtgtc tgtccggcct ggaactggtt tcacatacgg
      601 aaggattccg accgccaggc aaataataac aattccaacc catcgaacac gatataacat
      661 acttttctcc agtgaattgc aggctatccc caccagaaat aaaatcccaa aggacataaa
      721 ttattctgtg atttaaccaa aagactggtt gccaattgat cgctgaggac aatctgatat
      781 tccagacaca ctgatctgtt acacctggac caaaaggccg atggattggc cttttcaggg
      841 ttcctaattt gtgggtcttc aattttcagg caagcagttg aaaagcagga agctgacgag
      901 cttggatcaa tgtggggttc actttcttat gacatcttcc gaaagatatt tcaactgaac
      961 tgaagaaaat gacaaaatga cagtgtgaca ctgtgaccag atttgatcac aatgtcacac
     1021 tgtcatttgt cacgctgtta cctggtcaaa cggttacggt gttagttgat tttggacggt
     1081 gcccggaatt ggccaacctg gctgccaaga cggtttgggg cgccaaccag ttgccagaaa
     1141 ccagaactgg ataaagccgg ccaggcagtt gaactttggt tgttggcgtc acctaaatcc
     1201 agccctacga tccggttatt gccggtatct ccgactgaaa gcattaacct gcctgccaga
     1261 ggtcccgtag aaaagacact gaaggccact ccttcaggat tgcgaacctg tcccagtgcg
     1321 gaaccacttt gtgacaggag taccccagtt tcaccattcc caccaacaaa tttcagcaca
     1381 cgactattcc cagtgtcggc aataagcaga ttgccagaac catcaacagc aactccttg
//
LOCUS       DAOQDO010001960         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105314_length_1439_cov_3.489162, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001960 DAOQDO010000000
VERSION     DAOQDO010001960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105314_length_1439_cov_3.489162"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PKZ53_24125"
     CDS             <1..1072
                     /locus_tag="PKZ53_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNJ43590.1"
                     /translation="ANETVLKNLIIKELTDVKREFANERRTQIVDEETELTIEDLIPD
                     EEVVITITHGGYIKRTPLSSYRTQRRGGVGRRGMATKAEDVLDAMFIASTHSYMLIFT
                     DKGQVYKVKVHEIPDAATAGRGKAIVNLVELPQGEKVAGMFPIREFAEGRYVVMVTRR
                     GIIKKTELTEFANIRSNGIIAMSIEDGDVLMGIQMSDGNKHILLSTYHGMAIHFKETD
                     VRAMGRGAYGVIGINLRQDDYVTGVSVVDGTEHILALSELGLGKRTLVSEYPPQSRGG
                     VGVINMKVTDRTGGVITALPVKNDDQLMVITQQGQVVRIEVEPIRETSRSAQGVKIIN
                     LSEGDRVVSASLIDMQNAETSE"
BASE COUNT          362 a          330 c          391 g          356 t
ORIGIN      
        1 ggccaatgaa accgtgctca aaaacctgat tattaaagag ttaactgatg tcaaacgcga
       61 atttgccaat gagcgtcgga cacaaatcgt tgatgaagaa accgaactca ccatcgaaga
      121 cctgattccg gatgaagaag tcgtcatcac catcacccac ggtggctaca tcaagcgcac
      181 accgctttcg tcgtatcgca cccagcgacg cggcggcgtt ggtcggcgcg gaatggcgac
      241 caaggccgaa gacgtgctcg atgcgatgtt tattgcttcg acgcacagct atatgctgat
      301 cttcaccgat aaggggcagg tctataaagt caaggttcac gaaattccag atgcggctac
      361 cgccggacgc ggcaaagcga ttgttaatct ggtcgaattg ccgcagggtg aaaaagttgc
      421 gggcatgttc ccaattcggg agtttgccga aggccgctat gtggtgatgg tgacccggcg
      481 cggcatcatc aagaaaaccg aactcaccga atttgccaac atccggtcaa acggcatcat
      541 cgccatgagc attgaagacg gcgatgtgtt gatgggcatc cagatgagtg acggcaacaa
      601 acacattctg ctctcgacct atcacggaat ggcaattcac tttaaggaaa ccgatgtccg
      661 ggccatggga cgcggcgcct atggggtgat cggcatcaac ctccgacagg atgactatgt
      721 caccggagtt tccgtggtgg acggaacaga acacatcctg gcgctttcag aactcggcct
      781 tggcaaacgc acgctggtaa gtgaataccc gcctcaaagc cgtggtgggg taggggtcat
      841 caatatgaaa gtcacagatc ggacgggtgg tgtcatcacg gcgcttccgg tcaaaaacga
      901 cgatcaattg atggtgatta cccagcaggg acaggtggtt cggatcgaag tcgagccaat
      961 ccgtgaaaca agtcgctcag ctcagggagt caaaatcatc aacttgagcg aaggcgaccg
     1021 tgtggtttca gcctcattga ttgatatgca gaatgccgag acgagcgaat aggttcttgg
     1081 tgactggtga ctggtgactg gtgcctggtg cttggttctt ggttcttggt tctttgtgac
     1141 tggttcttgg ttcttggtgc ttggttcttg gttcttggtt cttggttctt ggttctttga
     1201 aagtattgat gccagttaag ggttgaaaaa attgttcgtt gaaaacaaag ggcttatcaa
     1261 aatctcaaat ttcagatgtt caatttgctg cctggaagtt gttgattttg aaatctcaat
     1321 ttcaactctt aattcgcatt attgattcct aagtacaaat ccccatcaat aggggatgga
     1381 atttggttct ttgatcgaat caacccggtt ttcgcccgga tgggcgctgg aaccaaact
//
LOCUS       DAOQDO010001961         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105316_length_1439_cov_3.367775, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001961 DAOQDO010000000
VERSION     DAOQDO010001961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105316_length_1439_cov_3.367775"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..608)
                     /locus_tag="PKZ53_24130"
     CDS             complement(105..608)
                     /locus_tag="PKZ53_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004721827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNJ43591.1"
                     /translation="MKLICLDVHYRDLDATAAGVVFDHWTDASPHLELTTTISPIAAY
                     ESGNFYRRELPCLQAILDLISDPLDVILVDGFVWLDHGAPGLGGHLYAALNQTIPVVG
                     VAKTKFLGATSVVEVFRGTSKTPLYISAAGIELAEAAQAIQHMHGPYRIPTLLKRVDQ
                     LSRKTSG"
     gene            complement(612..>1439)
                     /gene="rfbA"
                     /locus_tag="PKZ53_24135"
     CDS             complement(612..>1439)
                     /gene="rfbA"
                     /locus_tag="PKZ53_24135"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNJ43592.1"
                     /translation="HPITRVVSKQLLPVYDKPMIYYPLSVLMLAGIREILIISTPDDL
                     PKFVHLMGDGSQIGVQFTYAVQPRPEGLAQAFTIGRSFIGTDSVCLILGDNIFFGHGL
                     SELLKRAASLQSGGLVFGYPVSDPERYGVVDFDKEGNAISIEEKPQHPKSKYAVPGLY
                     FYDNSVVDIASKLKPSPRNELEITDVNREYLKRGTLRVELLGRGFSWLDTGTHEALQQ
                     ASLFVQVIQERQGLQVACIEEIAYREGFISADQLRSLAAPLLKSGYGKYLMDILNES"
BASE COUNT          384 a          363 c          359 g          333 t
ORIGIN      
        1 aggctgaaaa aaccagggct aggggatgag ggctaggggc aatcgaaggg atgagggatg
       61 aaagaaaacc aattcttccg cccctagccc tcagcccgct gtcttcatcc cgaggtcttc
      121 cgggacaact gatcaacgcg tttgaggagg gttggaatcc gatatggtcc gtgcatgtgt
      181 tgaattgcct gagcggcttc ggccagttcg attccggcag cactgatata gagtggcgtt
      241 ttgcttgttc ctcgaaagac ttcgaccact gatgtcgcac ccaaaaattt ggttttggca
      301 actcccacta ccggaattgt ttgattgagt gccgcataga ggtgaccgcc aagcccaggg
      361 gctccatggt caagccagac aaatccatcc acaagaatga catccagcgg atcgctgatc
      421 agatccagaa ttgcctgaag gcagggaagt tcacgccgat agaagttccc tgattcatag
      481 gcggcaattg gactgatggt tgtagtgagt tccaggtgtg gtgacgcatc agtccagtgg
      541 tcaaagacga cacccgccgc agtggcatcc agatcacgat aatgaacatc gaggcaaatc
      601 aatttcatgg ttcaagattc gttcaaaata tccattaaat atttgccata cccgcttttg
      661 aggagcggtg ctgccagact ccgtaattga tcagccgaaa taaacccctc gcgataggca
      721 atttcttcaa tacaggcaac ctgcagcccc tggcgttcct gaatgacctg aacaaacagc
      781 gatgcctgtt gcagagcttc gtgggtgccg gtatcaagcc aggaaaatcc ccggcccaat
      841 agctcgaccc gcagggttcc acgcttcaga tattcacgat tgacatctgt gatttccagc
      901 tcgttgcggg gagacggttt taatttcgat gcaatgtcaa ccacagaatt gtcatagaaa
      961 tagaggcccg gtaccgcata ttttgatttt gggtgctggg gcttttcctc aatcgagatc
     1021 gcatttcctt ctttatcaaa atcaaccacg ccataccgct cagggtcact caccggatag
     1081 ccaaacacca gtccgccaga ttggagtgat gctgcgcgct tcaataattc tgataaccca
     1141 tggccgaaaa agatgttatc gcccaaaatg agacacacgg aatctgtgcc aataaatgac
     1201 cttccgatgg taaatgcctg ggccagccct tcaggacgag gttgaaccgc gtacgtaaac
     1261 tggactccga tttgggaacc atctcccatc aggtggacaa acttgggcag gtcatccggg
     1321 gtcgaaataa tcaaaatctc gcgaatacca gccagcatca aaaccgataa cgggtaataa
     1381 atcatcggtt tatcatacac tggcaagagt tgtttgctga caacacgcgt aattggatg
//
LOCUS       DAOQDO010001962         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105456_length_1438_cov_3.014461, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001962 DAOQDO010000000
VERSION     DAOQDO010001962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105456_length_1438_cov_3.014461"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..641)
                     /locus_tag="PKZ53_24140"
     CDS             complement(60..641)
                     /locus_tag="PKZ53_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNJ43593.1"
                     /translation="MNRICVFCGSNPGTNPAYVEAAVTVGTLLASRQIGLVYGGGHVG
                     MMGTIADAVLARGGEAIGVIPVALAEKEVEHKGLTRLHVVKSMHERKALMADLADGFI
                     ALPGGFGTFEEYCEIITWAQLGFHHKPCGLLNVAGYYNHLLAMIDHGVDQQFIRPVHR
                     QLVIDDTDAERLLERMTTFVPPVIDKWIQRDET"
     gene            complement(752..1225)
                     /locus_tag="PKZ53_24145"
     CDS             complement(752..1225)
                     /locus_tag="PKZ53_24145"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNJ43594.1"
                     /translation="MVSVLFVCTGNICRSPSAEGVFQHLVDTAGLGEHIEVDSAGTHD
                     YHAGEPPDRRSQQAAARRGYNLSHLRAREVERGDFQRFDYLVAMDQGHYRYLRALCPR
                     GLDHKVKLFLDFAPHLNLKDVPDPYYGGAHGFENVLDLIEEAARGLLAEIQENHL"
     gene            complement(1240..>1438)
                     /locus_tag="PKZ53_24150"
     CDS             complement(1240..>1438)
                     /locus_tag="PKZ53_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009990089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HNJ43595.1"
                     /translation="IPGLEGYTGVLYRNPNDPRLWVPKLSGLGYTINFAHPKGWLVFL
                     AMIGIPLLIVLGTILLLASAR"
BASE COUNT          364 a          425 c          331 g          318 t
ORIGIN      
        1 aaccctgaac cctgaaccct gaaccctgaa ccctgaaccc tgaaccctga accccaggtc
       61 tacgtctcat cccgctggat ccatttatct atcaccggtg ggacaaaagt tgtcattcgt
      121 tccagcaacc gttcggcatc ggtgtcatca atcaccagtt ggcgatgcac gggacgaata
      181 aattgctgat ctacgccatg atcaatcatc gccagcaagt gattgtaata tccggctaca
      241 ttgagcagcc cacagggttt gtgatgaaac ccaagttggg cccaggtgat aatttcacag
      301 tattcttcga acgtaccaaa tcctcctggc agtgcaataa agccatctgc gagatcagcc
      361 atcagggctt tgcgttcgtg catggatttg acgacatgaa gcctggttaa acctttgtgt
      421 tcgacttctt tttccgccag cgcaacgggg atgacgccaa ttgcttcccc acctcgcgcc
      481 agcacggcat cggcaatggt gcccatcatt ccgacgtgcc cgccaccata gaccagccca
      541 atctgacgac ttgccagcag ggttccaacg gtcacagccg cttcaacata ggctggattc
      601 gttcctggat ttgaaccgca aaatacacaa attcgattca tgttattcct tccattgtaa
      661 gctgaagccg tcttcttcag ccctgagctt gcgaatcttc agccccaagc ccaatgtctt
      721 cagcccgatg tcttcagccc caggccccga tctataaatg gttttcctga atctcagcca
      781 gcaggccacg agcggcttct tcgataagat caagtacatt ctcaaaaccg tgggctccgc
      841 catagtaagg atcaggtaca tctttgaggt tgagatgcgg cgcgaaatcc aaaaaaagct
      901 ttactttatg atcaaggccg cgtgggcaga gtgcgcgcaa gtaacgataa tgcccctggt
      961 ccatcgccac cagataatca aaccgctgaa aatcgccgcg ttcgacttcg cgggcgcgca
     1021 aatggcttaa attgtagccc cggcgggccg cagcctgctg cgaacggcga tctggcggct
     1081 caccggcgtg atagtcatgc gtcccggctg aatccacttc gatatgttcg ccaagcccag
     1141 ccgtatcaac cagatgctga aacacacctt ctgccgaagg tgaccgacaa atatttccgg
     1201 tacagacaaa cagcacactc accatagcca accaactttc tatctcgccg aagcgagcaa
     1261 caaaatagtg cccagtacaa tgagcaatgg aattccaatc atggccagaa acaccagcca
     1321 gcctttgggg tgggcaaagt tgatcgtgta gccaagcccc gaaagctttg gcacccacaa
     1381 gcgtgggtca tttggattgc gatagagcac accggtatac ccttccagcc cagggatt
//
LOCUS       DAOQDO010001963         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105484_length_1438_cov_2.547361, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001963 DAOQDO010000000
VERSION     DAOQDO010001963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105484_length_1438_cov_2.547361"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..602
                     /locus_tag="PKZ53_24155"
     CDS             192..602
                     /locus_tag="PKZ53_24155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ43596.1"
                     /translation="MSETFPQEVQSKNRAVLLVDDEFSIRRVAGAVLEMNGYCVLSAS
                     NGAEALLIYQKHQKQIDLVITDWSMPVMNGKACIQEIRKVNLNVPIILVTASLEEIRD
                     VGMKELGIQALLGKPFTQFDFLKTVESVLESCNK"
     gene            839..1405
                     /locus_tag="PKZ53_24160"
     CDS             839..1405
                     /locus_tag="PKZ53_24160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ43597.1"
                     /translation="MAIKRQLVSQASIQKEHMVLDLGCGTATLTILIKQLHPEAVVYG
                     LDGDQKILELAQSKTTAANLDIQLVKGLVTELQLPNNVFNEVLSSLVFHHLSLEEKKL
                     ALREVFRVIKPGGSLHIADWGKPKNWRMRAVFLLVQLLDGFKTTRDSVTGILPELMGK
                     AGFENVQTTTDYNTPLGTLSLYRGCKPE"
BASE COUNT          427 a          270 c          345 g          396 t
ORIGIN      
        1 tggtggatgg tcattggcgc ctggctatcc ttacttgcag ggattggagg catgtggttg
       61 atctggaatc gccgcggaaa gaaatcagtt ttatctttca actaaaattt gtaatcaagc
      121 tcgaataatg agactaccca atcaccgtca gtgtcttttt tgatactgaa aagaatccag
      181 gcaggagata tatgagtgaa acttttcctc aagaggttca aagtaaaaat agggccgttc
      241 tcctggtaga tgacgaattt tctatccgaa gagttgctgg tgctgttttg gagatgaatg
      301 gatattgtgt tttgagcgcg agtaatggcg ccgaggcgct cctgatttat caaaaacatc
      361 aaaagcaaat cgatttggtg attactgact ggtcaatgcc tgtcatgaac gggaaagctt
      421 gtatccagga aattagaaaa gtgaatttaa atgtccccat tatcctggtc acagcgagcc
      481 tggaagagat tcgtgatgtt ggaatgaagg agttgggtat tcaagctctg ttggggaaac
      541 cgtttactca atttgacttt ctcaaaactg tggaatctgt acttgagtca tgtaacaaat
      601 gagttggttc tttggtattt gctctggaaa tgaacatacc ctcaaaaaca gaggtcggtt
      661 ctaaagagtt tacaggttca ggtcttgctc aaaaagctgg gcttcacggc aaataccaaa
      721 gagccaataa gttcgggaga aaaatgtgag tcagcaaaat tataacctta tacgagcact
      781 caaatttgat tttttgacac cagcctatga ttcagttttg ggttggactc tacctgaaat
      841 ggctataaaa cgtcagcttg tgagtcaggc aagtatccaa aaggaacata tggtgcttga
      901 tctcggttgt gggacagcaa cactgaccat attaatcaaa cagcttcatc cggaagcagt
      961 tgtctatgga ttggatggtg atcaaaagat tttagaactc gcccagtcca aaactacggc
     1021 agccaacctg gatattcaat tggtcaaagg gctagtgact gagcttcagc ttccgaataa
     1081 cgtttttaac gaagtgcttt caagcctagt ttttcatcat ttatctcttg aagagaaaaa
     1141 acttgcccta cgggaagtat ttcgagtcat caaaccagga ggaagtctcc atattgctga
     1201 ctggggaaaa ccgaaaaact ggcgaatgcg ggctgtcttt cttctggtcc aattgctgga
     1261 tgggttcaaa accacccgag atagcgtaac tggaatttta cccgagctga tggggaaagc
     1321 cgggtttgaa aacgtccaga caaccactga ttacaacact ccgttgggga ctctttctct
     1381 gtacagaggg tgcaaacccg aataagagaa aaaataaaga attcaggcat tggggtta
//
LOCUS       DAOQDO010001964         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105494_length_1438_cov_2.411424, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001964 DAOQDO010000000
VERSION     DAOQDO010001964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105494_length_1438_cov_2.411424"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKZ53_24165"
     CDS             <1..240
                     /locus_tag="PKZ53_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNJ43598.1"
                     /translation="DIAASLKVPVLGLYAEKDKGISLESVEQMRKQLKSPSEIVVFPG
                     VDHGFHADYRPSYNKEAAQDGWKRLLAWFKKYGAA"
     gene            complement(362..>1438)
                     /locus_tag="PKZ53_24170"
     CDS             complement(362..>1438)
                     /locus_tag="PKZ53_24170"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNJ43599.1"
                     /translation="AFITSAGSDSTIEEIGFLPFQIEMIYEKIAAATDGLLLVGGPTN
                     SGKNGTLKAITALLQQNYKGKRFASIEDPVEVGVPSMYQVSVSAKKQATDYLPALLRS
                     NFQGIQMTEIRDPETARFAFMAADTGIYTQATIHIRNPFQVIPRLSKYGISPNEISET
                     LIAVIYQRLLPRLCRECAIPEDWELLWKKYPRLKAILEYWQVPLHSFRPKRANKQGCP
                     HCIGGYRGRIGIFEIWNPELQRHQLSTNPDINEIRETALALGHWSMLRAGLCHAFAQI
                     VDIESVMKATNGVDPVYEGVEGYRYKDVEARIRDMEAEAAGVNNRKPSSEPPPASPSE
                     PAQPSAPVVDSHIIDAEFSDVEES"
BASE COUNT          333 a          356 c          390 g          359 t
ORIGIN      
        1 gatattgccg cgtcgctcaa ggtgccggta ctggggctct atgctgagaa agacaaaggc
       61 atctcgctgg aatctgtcga acaaatgcgc aaacagttga agtcaccatc tgagattgtg
      121 gtttttcctg gggtagatca tggcttccac gccgattacc gcccaagcta caacaaagaa
      181 gccgcccagg atggctggaa acgcctgctc gcctggttta agaaatacgg cgccgcgtga
      241 tctgacgcga tgagtttttg acaaaatgac acgctgacag gatgacaagg tgacaaataa
      301 caaggtgaca tctgtcattt tgtcatctgg tcacgctgtc accttgtcat tggtcacttc
      361 gtcagctttc ttccacatca gaaaattcgg cgtcaataat gtggctatca acaactggtg
      421 ctgagggttg ggccggttca gaggggctgg ctgggggtgg ttcagacgaa ggtttgcggt
      481 tgttgacgcc ggcggcttcg gcttccatgt cgcgaatgcg ggcttcgacg tctttataac
      541 gatatccttc aaccccctca tacaccgggt caaccccatt ggtggccttc atcaccgatt
      601 caatatccac aatctgggca aaggcatggc acagacctgc ccgcagcatt gaccagtggc
      661 ccagggccag agccgtctca cggatctcgt tgatgtcagg attggttgag agttgatgcc
      721 gctggagttc cgggttccag atttcaaaaa ttccgattcg gccccgatat ccgccgatac
      781 agtgcggaca gccctgttta ttggcccgtt tggggcgaaa tgaatgcagc ggcacctgcc
      841 agtattccaa aatggctttt aagcgcgggt actttttcca gagcaattcc cagtcttccg
      901 ggatcgcaca ctcacgacac aagcgtggga gcaaccgttg ataaataacg gcgatgagcg
      961 tttcgctgat ttcattggga ctgatgccgt attttgaaag gcgtggaatg acttgaaagg
     1021 gattgcgaat gtgaattgtc gcctgggtgt aaatcccggt atctgcagcc ataaacgcaa
     1081 agcgggcggt ttccgggtcg cgaatttctg tcatttgaat gccctgaaaa ttgctccgta
     1141 acagtgcggg cagataatct gtggcctgct ttttggccga aacgctcacc tgatacatcg
     1201 agggaacccc gacttccacc gggtcttcga tactggcaaa gcgtttgcct ttgtagtttt
     1261 gctggagcag ggcggtaatg gctttcaagg tgccgttttt cccgctattg gtcggaccac
     1321 cgacgagcaa tagcccgtcg gtcgccgctg caattttttc gtagatcatt tcaatctgga
     1381 agggcaaaaa gccaatttct tcaatagttg aatcagaacc agcagaggta ataaatgc
//
LOCUS       DAOQDO010001965         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105592_length_1437_cov_3.839363, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001965 DAOQDO010000000
VERSION     DAOQDO010001965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105592_length_1437_cov_3.839363"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKZ53_24175"
     CDS             complement(<1..341)
                     /locus_tag="PKZ53_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43600.1"
                     /translation="MNQKTEPQPDRRQNTKLNKPPVLFSKTQSIISKIERELGHTFIA
                     YWNSPGGSVCQNDVIGLYGVLRKLGHRDTIVLFIKSDGGSGQASLRMINLLRQYAGRL
                     VAVVPLTCASAA"
     gene            complement(473..1288)
                     /locus_tag="PKZ53_24180"
     CDS             complement(473..1288)
                     /locus_tag="PKZ53_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ43601.1"
                     /translation="MPDEPRNAIEFHDVVLSFGEQTILNGVSFVVKPGETKILMGGSG
                     TGKSTSLKLVLGLLKPDSGQIIVDGEDVTHFSENQLMKVRQKIGMVFQDGALFDSLSV
                     YQNVGFKMFEQGADEEEIEETVQRTLRFVNLEQAISKMPSELSGGMRRRVGIARALVG
                     NPKIILYDEPTAGLDPPTARTICELAMKLRDLEGVSSIFVTHRIQDATTLAHEHATIN
                     DMGELAICKNSERHHETNTQFVMLRSGKVIFEGDAQALWSSKDPYIQTFLDME"
     gene            complement(1315..>1437)
                     /locus_tag="PKZ53_24185"
     CDS             complement(1315..>1437)
                     /locus_tag="PKZ53_24185"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43602.1"
                     /translation="TSGGTVGVGRSTTQSVVVSILLILISDFFLTKLVLALSNF"
BASE COUNT          376 a          351 c          363 g          347 t
ORIGIN      
        1 gctgccgacg cacaggtgag ggggaccacg gccaccagac gtccagcata ctgacggagc
       61 aagttgatca tgcgcagcga tgcctgaccc gacccaccat cagatttgat aaacaacaca
      121 attgtatcgc ggtgaccaag tttgcgaagc acaccataca gcccgatcac atcgttttga
      181 caaaccgatc cgcccggtga attccaatag gcaataaatg tgtggcctaa ctcgcgctcg
      241 atttttgaaa taatgctttg ggtttttgaa aataaaaccg gtggtttatt gagtttggtg
      301 ttttgtcttc ggtctggttg aggctcagtt ttttgattca tttgcacatc catgaaaatc
      361 aggggtcagg gaaagcagaa aaggacataa aggatgaaaa agacgaaaag gacataaatt
      421 cgaaaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc cgctactcca
      481 tatccaaaaa cgtttgaata taagggtctt ttgaactcca cagtgcctgg gcatcgcctt
      541 cgaagatgac tttcccgcta cgcagcatca caaactgggt attggtttca tggtggcgtt
      601 cgctattttt acaaatagcc agttcgccca tgtcattaat ggtggcgtgt tcatgggcca
      661 gggtcgtggc gtcctggatg cggtgggtga caaagattga gctgacacct tccaggtcgc
      721 gcaatttcat cgccagttca cagatcgtgc gagcggttgg tgggtcaagt cctgccgttg
      781 gctcatcata caggatgatc tttggatttc ccaccagggc ccgggcaatt cctacgcggc
      841 ggcgcatccc gcctgagagt tcagacggca ttttactaat ggcttgctcc aggttgacga
      901 agcggagcgt gcgctggacg gtttcttcaa tttcttcttc atccgccccc tgttcaaaca
      961 ttttaaagcc aacattctgg tacaccgaga ggctgtcaaa gagcgctcca tcctgaaaga
     1021 ccatgccgat tttttggcgg accttcatca actggttttc agaaaaatgg gtcacatctt
     1081 cgccatcaac aatgatctga cctgaatcgg gtttgagcaa tcccaggaca agcttgagcg
     1141 aggtggattt tccggttccg gaaccgccca tcaaaatttt ggtttcgcct ggtttgacaa
     1201 caaaactgac gccattgagg atggtttgct ctccgaacga aagcacgacg tcatgaaatt
     1261 caatcgcatt gcgtggttca tctggcataa gttgggtggt aaaggcagca acaattagaa
     1321 attgctcagg gcaagcacca gtttggtcaa aaagaaatct gaaatcaaaa tgagtaaaat
     1381 tgagaccaca actgactgag tggttgagcg tcccacgcca acggtgccgc cagaggt
//
LOCUS       DAOQDO010001966         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105598_length_1437_cov_3.416787, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001966 DAOQDO010000000
VERSION     DAOQDO010001966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105598_length_1437_cov_3.416787"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKZ53_24190"
     CDS             <1..312
                     /locus_tag="PKZ53_24190"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43603.1"
                     /translation="IVVDNNGSEPVLNVTVKDVVPAALTYVSSSASQGTATVATENGM
                     PTVTFNFGAIPAKGKVTATMVAKTPLFIFGTVYNSATIVTGTGQPNRFNTASVDTLLV
                     L"
     gene            590..1270
                     /locus_tag="PKZ53_24195"
     CDS             590..1270
                     /locus_tag="PKZ53_24195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ43604.1"
                     /translation="MIQANPYTIKFKQQMQEMLQEGSIQARSLKVKRNDHIYTCGDHD
                     EMVYFIVSGQVKLLMVSPEGKECILAIHTAGDIFGELSLSGTGPRLETAAAMGTTILK
                     QIPSSRFVERLNRDSLVEGFIRYLAARIADQQQVIANLVTVDSEQRLGKTLLQLAHTF
                     GKKDPRSIRIELRISHEELSAMIGTTRPRVSVFMQRFKNMGLIELNADHYLIIKEDKL
                     TTYLESQA"
BASE COUNT          398 a          321 c          330 g          388 t
ORIGIN      
        1 attgtggttg acaacaacgg gtccgaaccg gtgttgaatg tgactgtaaa ggatgtggtc
       61 cccgcggccc tcacctatgt ttcttcttca gcttcacagg gaacggcaac cgtagccacc
      121 gagaatggaa tgccaaccgt gaccttcaac tttggggcaa ttccagccaa aggcaaagtc
      181 acggcaacca tggtggccaa aacaccgctc tttatttttg gaacagtgta caactcagcc
      241 accattgtca ccgggacggg tcaaccaaac cgattcaaca cggccagtgt tgatacgctt
      301 cttgtcttgt aatccgcctc caaactccat acgtgtgcta gatcaaaacc aaaccgggaa
      361 gggctctcgc aatgagttct tcccggtttg gttttgttca tttttgtctc aacacgacct
      421 caatttggct catattcacc aaatatgaag cgtgactgga ttttctcgcc gggttttcta
      481 taatttttta tacagtcttg aaatataagg gtattgataa tttcatttgt cagtcaatga
      541 gatcagcgag ttctgtaatc actcatttat tttttaaagg aggttaccta tgattcaagc
      601 aaacccttat acaatcaaat ttaaacaaca aatgcaggaa atgctccagg aaggaagcat
      661 ccaggcacgt tctctcaaag tgaaaaggaa cgatcatatt tatacgtgcg gtgaccatga
      721 cgaaatggtg tattttattg tcagtgggca ggttaagttg ttgatggtct caccggaagg
      781 aaaagaatgt attctcgcta ttcacactgc cggagatatt ttcggagagt taagtctgtc
      841 agggaccggc ccacggttgg aaacggcagc cgctatggga acgacaatcc tcaaacaaat
      901 tccaagctcg cggtttgttg aacggttaaa tcgggactca ctggttgagg gctttatccg
      961 ctatctggcg gctcggattg ccgaccagca acaggtcatt gcaaacctgg tcaccgtaga
     1021 cagcgagcaa cggttaggaa agacgctgct ccaactcgct catacatttg ggaaaaaaga
     1081 ccctcgcagt attcggattg aactcagaat ttcacatgaa gaattatcgg caatgattgg
     1141 cacaacccgt ccccgagtga gcgtttttat gcagcggttt aagaatatgg gattgattga
     1201 actgaacgcc gaccattatc tcattatcaa agaagataaa ctgaccacct atctggaatc
     1261 acaagcttga taccaattgg ggctaagtac aaatccccat aaatgaggga tgaaattcgg
     1321 ttctttgatc gaatcaaatc ggttttcgcc cagtgagtgg ttgaagttca tcccccattg
     1381 atggggattt ttgtactaag ggatcgttaa aatttaactt cccgttttcg ttggggg
//
LOCUS       DAOQDO010001967         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105599_length_1437_cov_3.405210, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001967 DAOQDO010000000
VERSION     DAOQDO010001967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105599_length_1437_cov_3.405210"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..>1437
                     /locus_tag="PKZ53_24200"
     CDS             275..>1437
                     /locus_tag="PKZ53_24200"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ43605.1"
                     /translation="MKLVITYLQGSLANTRSEIGDPLLYIGRDPSQCRIVFGENDAGV
                     SRRHAEIRLHNESLVLTDLQSTYGTFVNNYRIDRPVLLTPGCIVQFGQGGPVIRIDSF
                     EPHGQVTVAEAPQTPPVSPAAGVSSSPNAIPAASPPVQPMTRPPSGMPGPVSQGGSHQ
                     PPASVRFTPPAEPQPPLSPTPSIPPSVSSLSSPPSGAGNGPVTVSVYLELLNGNRTEQ
                     FKLMTSGTMLLGREETNQIRLDPTVFPTVSRRHAEIQARNGEYLLCDLGSFNGTLLDG
                     QRIASPTPLRHGNRIQLGFGGPEFRFVNPTQAAAMVIASAHEEGAGTVVIRGQKPVSS
                     VAPGERRQFVVRKEFTKDILTLGRSSDTDLQLDVLQISKHHARFVRQGGTVTV"
BASE COUNT          297 a          389 c          381 g          370 t
ORIGIN      
        1 cacggtgtca ttctgtcatt ttgccaccca gccagaagcg ccccttgttc cttgttccag
       61 ccctatagta cattccagcc tgcattcatt ctcacggttc ctcatctctt tcccgaaaat
      121 ttgacccctg gttgtgattg gatccatcat tgtctggcct gactccggac ctgtgtggat
      181 ctttgcaaat cctgtggcgt gttttgacag actcccatct gttgtgccgt ggatcaaagc
      241 tgagctggtg tctgcgtttt gaggcgacaa tcccatgaaa cttgtcatta cctatctaca
      301 gggatctttg gccaacacac gaagtgaaat tggcgatcct ttactttata ttggccgtga
      361 tcccagtcag tgccggattg tatttgggga aaatgatgct ggtgtttcgc gtcggcacgc
      421 tgaaatccgt ctccataatg agtcactggt tttgaccgat ctccaaagta cctatggcac
      481 ctttgtcaat aactaccgaa ttgaccgacc tgtgttactg acaccagggt gtattgtgca
      541 gtttggacag ggggggccag tgattcggat tgatagtttt gaaccacatg ggcaagtaac
      601 tgtagctgaa gcacctcaga cacctccggt atcaccggct gccggggtca gctcaagtcc
      661 aaacgcgatt ccggctgctt caccaccagt tcaaccaatg acccgaccgc cttccggaat
      721 gcccggtcca gtttcacaag gcgggagcca tcagccgcca gcttcggttc gatttacccc
      781 gccggctgag ccgcagcctc cgctttcccc gacgccttcg attcctccct cagtttcgtc
      841 actttcatca ccgccatctg gtgccggaaa tgggccggtt accgtgagcg tgtacctgga
      901 actcctcaat gggaaccgaa cggaacagtt caaactgatg acaagtggca cgatgttgct
      961 tggccgcgaa gaaaccaacc agattcggct ggatccgacg gtatttccaa ccgtgtcgcg
     1021 ccgccacgct gaaattcaag cccggaatgg cgagtatttg ctgtgtgatt tggggtcgtt
     1081 taacggcact ttactggatg ggcagcgaat tgcttcgccg acacctttgc ggcatggcaa
     1141 tcgaattcag ctcggctttg gtgggccaga atttcggttt gtgaacccga cccaggcggc
     1201 agccatggtg attgccagtg cgcatgagga aggtgcgggg acggtggtga tccgtggcca
     1261 gaaacctgtg agcagcgttg ctcctggtga gcgccgtcaa tttgttgttc gcaaagagtt
     1321 taccaaagat atattgaccc tggggcgaag ttctgacacg gatttgcagc ttgacgtttt
     1381 gcagatttcc aaacaccacg cccggtttgt gcgtcaaggc gggactgtga cagtgga
//
LOCUS       DAOQDO010001968         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105603_length_1437_cov_3.286541, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001968 DAOQDO010000000
VERSION     DAOQDO010001968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105603_length_1437_cov_3.286541"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1324
                     /locus_tag="PKZ53_24205"
     CDS             302..1324
                     /locus_tag="PKZ53_24205"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNJ43606.1"
                     /translation="MDFKDFWYIAAESKDLRPDRPWPVQILGNWVVLFRDEKGRPVAL
                     EDRCLHRGARLSTGKVTAGRLQCGYHGWMYDGLGQVVNVPSEGPESKVRKSRCARRFP
                     VCEQDGYLYVRLSESPESEVPPFSIPFYQTPGWAAIRLKNWFHNTVTNCVENFVDIPH
                     TTFVHPRIFRDAKAERFTAEVTRKHGSVVVQYHNERANFGWFSKFLNPTAREIQHTDS
                     FHLPNITSVDYIFSPKRRFIITSQSIPVSDDETLVYTDLTYNYGIWNLLARPVIRWLA
                     QRIIDQDMAILKNQMQTIRQFGAEFSNTEADTIHVLIESIRNELAAGRDPRLLPEKRV
                     EIEFWV"
BASE COUNT          345 a          374 c          373 g          345 t
ORIGIN      
        1 gattgaaatc gaaaacgtct cggacggcct cctggctgac ccgctccccg atgaactcga
       61 agtcctcgac atcgttgccc ccgccaaccc acgggcccgc cttgcctgtc aattgcaact
      121 gacagcttca ctcaaggtga aatgcctggg ctgagaaaac cagggcttgg ggctcatggc
      181 tgaagactcg caagctcggg gcggaagaaa atgggttcaa taccctgggc tcgatgtctt
      241 cagcccaact tcttcagccc gatattcttc agcccgatgt cttcagcccc actaaacgcc
      301 catggatttc aaagatttct ggtacattgc cgccgaatcc aaagacctcc gaccagaccg
      361 cccctggcca gttcagattt tgggcaactg ggtggtactg tttcgtgatg aaaaaggccg
      421 tccagtcgcg ctcgaagatc gttgcctgca tcgcggtgcc cggctttcga ctggaaaagt
      481 aactgccggg agactccagt gtgggtatca cggctggatg tatgatgggc tgggacaggt
      541 agtaaatgtt ccatctgaag gcccggaaag caaagttcga aaaagtcgct gtgctcgtcg
      601 ctttccggta tgtgagcaag atgggtatct ctatgtgagg ctttctgagt cacctgagtc
      661 tgaagtcccg ccgttttcaa ttccctttta tcaaactcca ggctgggcag cgatccggct
      721 caaaaactgg tttcacaaca cggtgaccaa ttgtgtcgag aattttgtgg atattccgca
      781 tacgaccttt gtccatccgc gaatttttcg ggatgcgaaa gctgaacgat ttaccgctga
      841 ggtgacccga aaacatggtt cggtggtggt tcagtatcac aacgaacggg ccaatttcgg
      901 gtggttttca aaattcctga acccaacagc tcgtgaaatc cagcacacgg attcgttcca
      961 tctgccaaat atcacttcag ttgattatat tttcagcccg aagcgccgct ttatcattac
     1021 ttcacaatca attccggtga gcgatgacga aacgctggtt tatacggatt tgacctataa
     1081 ctatgggatc tggaatctcc tggcgcggcc agtgattcgc tggctggccc agcgcattat
     1141 tgaccaggat atggctattt taaagaacca gatgcagacc attcgccagt ttggagctga
     1201 attttccaac accgaggccg acaccatcca tgtcttgatc gagtcaatcc gcaacgaact
     1261 tgcggctggt cgtgacccgc gcctgctccc tgagaagcgg gtcgaaattg aattttgggt
     1321 gtgaattttc gggttcaggg ttccgggttc agggttccgg ggttcgggaa agggcaaaag
     1381 gaacataaag gacataaagg acataaattc gaaaacccgg aacccggaac ccggaac
//
LOCUS       DAOQDO010001969         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105644_length_1437_cov_2.368307, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001969 DAOQDO010000000
VERSION     DAOQDO010001969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105644_length_1437_cov_2.368307"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PKZ53_24210"
     CDS             complement(<1..1081)
                     /locus_tag="PKZ53_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ43607.1"
                     /translation="MRSLGKLSWIKLLSCGLVIGLALIVFGVRWNGARTQAASSSPTQ
                     PANETDLATDPETAATASAPAPSKDAPKSTKAGDRYAQDADDSGCKSCHQGVEPSHVN
                     IKGQEDPVISCVYCHGGKGDVKAPAGEQMGATGYEKAKNEAHVQPKYPKKWQDAKTKK
                     QTSANPVRSYELLNQEDPKFIRFINPGDLRVADVSCGECHAEEVKFVRTSMMTHGGML
                     WGAALYNNGAYRVKNSQFGEAYNVDGAPIRLETHPKPTPEETKYKGVIPFLNPLLRWE
                     VSQPGNILRVFERGGKKLPEVGNPIPDERPGLPEVKLSPRGFGTVLRTDPVFLGLQKT
                     RLLDPLLSFLGTNDQPGDYRSSGCTA"
     gene            complement(1155..>1437)
                     /locus_tag="PKZ53_24215"
     CDS             complement(1155..>1437)
                     /locus_tag="PKZ53_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43608.1"
                     /translation="EVNFNYMRFHRTQVLKELLFQPRIRHNIGFDYGASLIYRPLLSE
                     NIIFKSGISVFNPLSGFTDIYTSSCEPGVNCGQGQKTLFALFSKVIVTF"
BASE COUNT          316 a          377 c          362 g          382 t
ORIGIN      
        1 aggcagtaca accgctggag cggtaatccc ctggttgatc gttggtgccc aggaacgaca
       61 gtaatggatc cagcaaccgt gtcttttgca gccccaggaa caccgggtca gtgcgcagca
      121 ctgttccaaa tccacgcggg ctgagtttga cttcgggtaa tccagggcgt tcgtccggaa
      181 tcgggttgcc aacttcgggc agtttcttac ctccgcgttc aaacacccgg aggatgttgc
      241 ccggttgaga aacttcccac cgcagcaacg ggttcaaaaa tggaatcacg cctttgtact
      301 tggtttcctc tggcgttggt tttggatggg tttcaagccg gattggggcg ccatcaacat
      361 tgtacgcctc cccaaactgc gaatttttta ctcgatacgc cccattatta tagagcgcgg
      421 ccccccacag cattccgcca tgggtcatca tgctggtgcg gacaaacttc acttcctcag
      481 cgtggcactc gccacacgag acatcagcca cgcgcaggtc gccagggtta atgaagcgga
      541 taaattttgg atcttcctga tttaacagtt catatgatcg gaccgggttg gccgaagttt
      601 gttttttggt ctttgcatcc tgccatttct tggggtattt cggctgtacg tgggcctcat
      661 ttttggcttt ctcatagcca gtagccccca tttgctcacc agcaggggct ttgacatcgc
      721 cctttccacc gtggcagtac acgcatgaaa taactggatc ttcctgacct ttaatgttga
      781 cgtgggatgg ttcaaccccc tgatgacagc ttttacatcc tgaatcatct gcatcctgtg
      841 catatcgatc accagctttg gtgctcttgg gtgcatcttt ggatggcgct ggtgcagatg
      901 cagtcgccgc tgtctcgggg tcggttgcca agtcggtctc gtttgcgggt tgggttggtg
      961 atgaggaagc ggcctgagtc cgggcgccat tccatcggac gccaaaaacg atcagcgcca
     1021 gcccaattac caggccacaa ctcagtaact tgatccaaga gagtttcccg agtgaacgca
     1081 tgtgtctttc cctctcttct tgatatgaaa gcgatcagtt cacaactggt ttttttgttt
     1141 gataacgtct cagattagaa cgtcacaata acctttgaga acagcgcaaa cagtgttttc
     1201 tggccctggc cgcaattcac accaggttca caacttgaag tataaatgtc agtgaatccg
     1261 ctcaatgggt taaacactga aatcccggac ttgaaaatga tgttttcact tagtagtggc
     1321 cggtaaatca gcgacgcgcc atagtcaaac ccgatattgt ggcgaatcct cggctggaag
     1381 agtaactctt tgagcacctg ggtccgatgg aagcgcatat agttaaaatt cacttcc
//
LOCUS       DAOQDO010001970         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105723_length_1436_cov_4.775525, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001970 DAOQDO010000000
VERSION     DAOQDO010001970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105723_length_1436_cov_4.775525"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1436)
                     /locus_tag="PKZ53_24220"
     CDS             complement(66..>1436)
                     /locus_tag="PKZ53_24220"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNJ43609.1"
                     /translation="GVAGQKIDADLEIAGIADYVAMRVLQCHLDTMERLGIRYDVMPR
                     ESEVLHMHLWDAAFERLKASGSIIYETSGRNKGCWVMKAEQSETTQDEESEHDADKIL
                     VRSNGTVNYTGKDIAYHLWKLGQLDLEFHYKPFHTYPDGKVVWITTPTLPENAAELPS
                     RPAFGNGSAYFNVIDVGQSYTQEYVKKGVLAALVNQMDTHRVERSAHLSYEKVALTPN
                     SCDVLGFELDEEERKRTIVSMSGRKGLGVKADDLLDRLETSALVHVTEHQATLPPEEQ
                     KSPEAQQAMAHTVAVGALRYFLLKYTRGTIIAFDFKEALKEQGETGIYLQFAAVRINS
                     IYRKANRRGISTDDPLSVLKANPEKLQTFFTGEDGQFFWGLTSLSLQLNEVLVQAAEF
                     LEPAQIAKYAFQIAQTMSSFLNKYHVLGEADPVRQQFYLALLALVRRQLQTAMHVMGI
                     DVPEQM"
BASE COUNT          334 a          373 c          365 g          364 t
ORIGIN      
        1 ccaagaacca agaaccaaga accaagaacc aagaaccaag aaccaagaac caagccttcg
       61 gcttgctaca tttgctccgg cacatcaatg cccatcacgt gcatggcggt ctgcaattgg
      121 cgccgaacca gcgcgaggag tgccagataa aactgctggc ggacagggtc agcttcgccc
      181 agaacgtggt atttatttaa aaagctgctc atggtttgag caatctggaa ggcatatttg
      241 gcaatttgag ctggttccag gaattcagcc gcctgaacca gaacttcatt caattgaagc
      301 gaaagactgg tcaatcccca aaaaaactgg ccgtcttcac cggtaaagaa ggtctggagc
      361 ttttcgggat tagctttcaa caccgacagc gggtcgtcgg tggaaattcc acggcggttg
      421 gctttgcgat aaatgctgtt gattcggaca gcggcaaatt gcaggtaaat gccggtctcg
      481 ccctgttctt tgagcgcttc tttaaagtca aaggcaatga ttgtgcctct ggtgtatttg
      541 agcagaaagt accggagtgc accaaccgct accgtgtgcg ccatggcttg ttgggcttcg
      601 ggagattttt gctcctcggg cggcagtgtc gcctgatgct cggtcacatg aaccagcgcg
      661 ctggtttcca gtcggtccag caggtcatcg gctttgacgc caagtccttt gcgaccactc
      721 atgctcacaa tggttcgttt gcgttcttct tcgtccagtt caaacccgag cacatcacaa
      781 ctattcggag tgagtgccac tttttcatag ctcaggtggg cgcttcgctc aacgcgatgg
      841 gtatccatct gattgacgag tgctgccaga acgccttttt tgacatattc ctgggtatag
      901 ctctggccga cgtcaatcac gttaaaataa gccgagccat tgccaaatgc cggtcgtgat
      961 ggaagttctg ctgcgttttc aggcagagtc ggagttgtaa tccagacaac tttgccgtcc
     1021 gggtaggtat gaaacggctt gtaatggaac tcaaggtcaa gttgccccag cttccagaga
     1081 tggtaggcaa tatctttccc cgtgtagttg accgtgccgt ttgatcgaac caggattttg
     1141 tcagcatcgt gttctgattc ttcgtcctgt gtggtctctg attgctcggc tttcattacc
     1201 cagcagccct tgttgcgacc agatgtttca taaataattg aaccagaagc ttttaatcgt
     1261 tcaaaagctg catcccaaag atgcatgtgc aagacctcgc tttcgcgagg catcacgtca
     1321 tagcgaatcc ccagccgttc catcgtgtcg agatggcatt gcagaacgcg cattgccaca
     1381 taatcggcaa tcccggcaat ctcgaggtca gcgtcgatct tctgtcccgc gacgcc
//
LOCUS       DAOQDO010001971         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105724_length_1436_cov_3.900072, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001971 DAOQDO010000000
VERSION     DAOQDO010001971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105724_length_1436_cov_3.900072"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKZ53_24225"
     CDS             complement(<1..427)
                     /locus_tag="PKZ53_24225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger protein"
                     /protein_id="HNJ43610.1"
                     /translation="MTIREGKWKCTYCDMLNRGRDLKCTGCGAVREQDVQFMYDENAP
                     EITDQAELASARRGPDWICETCGVSNLSTAEGCRQCGAPRGGSVNREVGLVPPPDAQS
                     MMPEPPTTSGKSWMVKGLLGCGGLVAVAVILFLGLGIYLT"
     gene            586..1389
                     /locus_tag="PKZ53_24230"
     CDS             586..1389
                     /locus_tag="PKZ53_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ43611.1"
                     /translation="MSEENPSVPVIEAIGLQKSYGHHQAVKGVDLVVRPGEIFGFLGP
                     NGAGKTTTIKMLVGLLAPSAGVARIGGHDIQKEPIQAKSLIGYVPDQPHLPDKLTARE
                     YLRFIAGLYNLDRTAARRRGEELLKLFALSDQADELVGGYSHGMRQKIVLAGALLHDP
                     KVFFLDEPTVGLDPRSARLIKDILRDLAGRGTAVFMSTHILEIAERMCDRVAIIYDGR
                     VIAIGTMEELRTAQTAESLEDIFLKLTGGTDEKASGELSIKRGFENPEP"
BASE COUNT          344 a          368 c          379 g          345 t
ORIGIN      
        1 gtgtgagata aattccaaga ccaagaaaga ggataaccgc aacggccacc aggccgccgc
       61 atccgagtaa ccccttgacc atccaggatt tcccgctggt cgtgggtggt tcgggcatca
      121 tggattgggc gtcaggtggt ggaaccaggc ccacttcacg attgactgaa cctccacgcg
      181 gagcaccaca ttgccggcat ccttcagccg tactgagatt tgagacacca caggtttcac
      241 aaatccaatc cggcccgcgt cgagccgatg caagctcagc ttgatcagtg atttcgggag
      301 cattctcatc atacatgaat tgcacatcct gctcacgaac tgccccacat ccggtgcact
      361 ttaaatctcg tccacgattg agcatgtcac agtaggtaca tttccatttt ccttcacgga
      421 tcgtcatagc cagggttcct tgtgctgatg taaggtgtga aaactgagac catttggggc
      481 tgagggcctg gggttgagcg aattccaatt ctctcaacga tgtcaccagt tgccgctatc
      541 ctggagcgac tccaaactgg tcatatcaat ctttcggagt tattgatgtc tgaagaaaat
      601 ccaagtgtac ctgtgattga ggccattggt ctgcagaaaa gttatggtca ccatcaggca
      661 gtcaaagggg ttgatctggt ggttcgtcct ggtgaaattt ttggcttttt gggccccaac
      721 ggcgctggga aaacaaccac gatcaaaatg ctggttgggt tgcttgcacc atcggctggg
      781 gtggctcgga ttggcggcca tgatatccaa aaagaaccca tccaggccaa gtctttgatt
      841 ggatatgtcc ccgaccagcc gcacctgcca gataaactga cggccaggga atatctgcga
      901 tttattgctg gtctgtataa tctggatcgc acggctgccc gtcgccgggg agaggaattg
      961 ctcaaactct ttgctctgag cgatcaggct gacgagctgg tcggtggata ttcacacggt
     1021 atgcgccaaa agatagttct tgcgggggcg ttactccacg atcccaaagt gttttttctg
     1081 gatgaaccaa ccgttggcct tgacccacga agcgcccgtc tgattaaaga cattttgcgt
     1141 gacctcgctg gacgtggaac ggcagtcttt atgtcaaccc acattcttga aattgctgag
     1201 cggatgtgtg accgggtggc gattatttat gatggacggg tgattgccat tggcaccatg
     1261 gaggagcttc gcacggctca aaccgctgaa agcctggaag atattttcct gaaactgact
     1321 ggcggaactg atgagaaagc gagtggtgag ttgtcaatca aaagagggtt cgaaaaccct
     1381 gaaccctgaa ccctgaaccc taccccctga accctgaacc ctgaaccccg aactat
//
LOCUS       DAOQDO010001972         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105755_length_1436_cov_2.864591, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001972 DAOQDO010000000
VERSION     DAOQDO010001972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105755_length_1436_cov_2.864591"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="aceA"
                     /locus_tag="PKZ53_24235"
     CDS             complement(<1..719)
                     /gene="aceA"
                     /locus_tag="PKZ53_24235"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNJ43612.1"
                     /translation="MTVYEMYENWKTNERWAGIERPYTPEDVLKLRNTVEIKYSLAEL
                     GAKRLWDLLNTEDYVHALGALTGNQALQQVKAGLKAIYLSGWQVAADANLAGQMYPDQ
                     SLYPSNSVPSVVRRINQTLLRADQIYHSEGKDGTYWLAPIVADAEAGFGGPLHAYELM
                     KAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTSHFIRILTAARLAADVMNVPSLVI
                     ARTDADSAQLLTSDVDPADHR"
     gene            complement(858..>1436)
                     /locus_tag="PKZ53_24240"
     CDS             complement(858..>1436)
                     /locus_tag="PKZ53_24240"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNJ43613.1"
                     /translation="DAQFAKVREDKELEASMGHDGCWVSHPFFIGIAMSAFIRDNQLD
                     VLLEDFDKYPDLIPEPSGAKTLAGVRKNVRVGIAYQNGWNQDIGCVAWDNLMEDLATL
                     EISRAQTWQWLHNRVTLDDGQTVTPELIKQVFEEEYNRIVTEIRDEMAGAPEARVEAV
                     IAGFLKAREDAEALFTKEEFPDFLSLSSDFAS"
BASE COUNT          358 a          392 c          326 g          360 t
ORIGIN      
        1 aaccgatgat cagccggatc aacgtcgctg gtcagcaact gagcgctatc agcgtctgtg
       61 cgtgcaatca ccagcgatgg gacgttcatc acgtcagcgg caagccgggc cgctgtcagg
      121 atccgaatga aatggctggt cggcaccagg acttttccac ccaggtgacc acatttcttt
      181 tccgaagcaa gctggtcttc aaagtgaacc ccagccgcgc cggcttcgat catcgctttc
      241 atcagttcat atgcgtgcaa cgggccgcca aatccagctt ctgcgtctgc aacaataggt
      301 gccagccaat aggtaccatc ttttccttca gagtggtaaa tttggtcagc ccgaagcaac
      361 gtctgattga tacgacgtac aacacttgga acactgtttg aaggatacaa actctgatct
      421 ggatacattt gcccggctaa atttgcatct gctgcgacct gccaaccgct gagataaatg
      481 gccttgagtc cagctttgac ctgttgcaac gcctgattgc cggttaaggc acccagcgca
      541 tgcacataat cctcagtgtt caacagatcc cacaaccgct tggcgcccag ctccgccagt
      601 gaatatttga tttcaacggt attgcgcagc ttcaatacat cttccggggt ataaggccgc
      661 tcaattccag cccagcgttc gttggttttc caattttcat acatctcgta aacggtcata
      721 agtttttgta tcctttccag cgtaagtaaa ttcttctttt actggcactt cgacgttgta
      781 cttcgttcaa aaaattaaga taaagacgtg ggcagtaaga acaatgcgac gacgtaccag
      841 acactccaac atcagaatca ggaggcgaaa tctgatgaca aacttagaaa atcagggaat
      901 tcttctttgg tgaagagcgc ttcggcatct tcacgagctt ttagaaaacc ggcgatgacg
      961 gcttcaactc tcgcttcagg cgcgccagcc atctcgtcac ggatttcagt cacgatccgg
     1021 ttgtattctt cttcaaagac ctgtttgatc agttcagggg taaccgtctg accgtcgtca
     1081 agcgtcaccc ggttatgaag ccactgccag gtctgagcgc gtgaaatttc cagggtggcc
     1141 agatcttcca tcaaattatc ccaggcgaca cagccgatat cctggttcca gccgttctga
     1201 taggcaatcc caacccgaac gtttttgcgc accccagcca gcgttttggc acccgatggc
     1261 tctggaatca gatccggata tttgtcgaaa tcttccagga gcacatccaa ctggttatca
     1321 cggataaaag ccgacatggc aatgccaatg aaaaatgggt gtgataccca gcagccatca
     1381 tgccccattg aagcttcaag ttctttatct tctcgaactt tggcaaattg cgcatc
//
LOCUS       DAOQDO010001973         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105813_length_1436_cov_2.107893, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001973 DAOQDO010000000
VERSION     DAOQDO010001973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105813_length_1436_cov_2.107893"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..>1436)
                     /locus_tag="PKZ53_24245"
     CDS             complement(45..>1436)
                     /locus_tag="PKZ53_24245"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43614.1"
                     /translation="GGVLYFFLSKTFLNLVDSSLQFHVRALAANATASPTIENEAEFA
                     ESPTVPASPESGALVLPPQLIQFVDETGNVSQQVLEQSEQTLLGKSPTKPLGNWREAG
                     RLSSPYFETIELARGHRLRLATLAVRSEGQLELFITVGQELDGLIRLQNTVLQVVGVS
                     IPIILVLLGSGGWLLVTQTLKPVDQLTRAAQRIGEGNLRELVQTPKTGDELARLAETF
                     NQMITRLNVAFERQRQFTADASHEFRTPLAIMRSELELALQRERPAEDYQQTLESLLE
                     ETRRLNKLVDDLLTLARSDSGELALESNPVDLGALCTEVTEAFMPLAELREQQLRRTL
                     PEGKAMVSGDQQWLRRLLLNLLDNALKYTPSGGQIELEVEVIPGDASHQSQIRVLICD
                     TGPGIPQADRERIFDRFYRHCHTGSNEQGGFGLGLSICRWIVQAHYGTLKLLESEHGA
                     VFEVVLPGMKNEE"
BASE COUNT          340 a          417 c          341 g          338 t
ORIGIN      
        1 cccggaactg gtttcttcat tcttcattct tcattctcaa ttcttcattc ttcattcttc
       61 atgcctggca gcaccacctc aaaaaccgct ccgtgttcac tttccaataa tttcaaggtt
      121 ccataatggg cctgcacaat ccaccggcaa atagacaacc ccaatccaaa cccaccttgc
      181 tcgttgcttc cggtgtggca atgacgataa aaccggtcga agatccgttc ccgatctgcc
      241 tgcggaatgc caggcccggt atcgcaaatc agcacccgaa tctgtgattg atgtgatgca
      301 tccccaggta taacctcaac ctcaagttcg atttgtccgc ctgacggagt gtatttgagg
      361 gcattatcca gcaaattgag caataaccgg cgcaaccact gttgatctcc gctgaccatg
      421 gcttttcctt ccggtaacgt gcggcgcaac tgttgctcac gaagttccgc cagcggcata
      481 aatgcctcgg tgacttcggt acagagtgcc ccaaggtcaa ccgggtttga ttcgagcgcc
      541 aactcgccac tatcagacct cgccagagtt aaaagatcat caaccagttt gtttaagcgt
      601 cgggtttctt cgagcaggct ttcaagggtt tgctggtaat cttcggctgg tcgttcgcgc
      661 tgcagagcaa gctcaagctc actgcgcata attgccagcg gcgtgcgaaa ttcatgcgag
      721 gcatccgccg taaattgacg ctgtcgctca aaggcaacat taagtcgcgt gatcatctgg
      781 ttaaaggttt ccgccagccg tgccagttca tcgccagttt ttggtgtctg caccagctcc
      841 cgaagattcc cttccccaat gcgctgggcg gcgcgggtca actgatcaac cggcttgagc
      901 gtctgtgtca ccagcaacca cccgccgctt cccaacaaga ccaggatgat ggggatactg
      961 acaccgacga cctgtaaaac cgtgttctga agccggatca aaccatccag ttcctgccca
     1021 acggtgataa ataattccaa ctggccttca ctccggaccg ccagcgttgc caatcgcagg
     1081 cgatgaccgc gagccagttc aattgtttca aaatacgggc ttgaaagccg acccgcctcg
     1141 cgccagttgc caagtggttt ggtcggggac ttaccaagca acgtttgttc tgattgctcc
     1201 agcacctgct gcgatacatt tccggtttca tccacaaact gaatcaattg tggcgggagc
     1261 accagcgcac cagattcagg cgacgcagga accgtcggtg actcagcaaa ctcagcctca
     1321 ttttctatgg tggggctggc tgtggcattg gcagccaggg ctcgaacgtg aaattgaagc
     1381 gacgaatcaa ccagattcag aaatgttttc gagaggaaaa aataaagcac accacc
//
LOCUS       DAOQDO010001974         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105896_length_1435_cov_3.054348, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001974 DAOQDO010000000
VERSION     DAOQDO010001974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105896_length_1435_cov_3.054348"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..515
                     /locus_tag="PKZ53_24250"
     CDS             69..515
                     /locus_tag="PKZ53_24250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43615.1"
                     /translation="MNIFLWTAGMSALAILVTCLVSLIVMRKIRQAIQTAEEKVRETE
                     NKLLSSKEELNSLSKRVVEQSSRIAWLESRVRNKPVPAGTQSPEPEPQAGPTKLSLTE
                     RRHRVLSLARRGFDTDAISSLLNTPNGEIELILSLTTQGAGLRVEG"
     gene            592..1383
                     /gene="flgF"
                     /locus_tag="PKZ53_24255"
     CDS             592..1383
                     /gene="flgF"
                     /locus_tag="PKZ53_24255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNJ43616.1"
                     /translation="MDHGLYTAYLGLRARQNALDVTANNIANASTPGFKGDYMLYRSI
                     QLSAQEQSAAPTGINPQAITQLDPSQAAAHTRSTGLVTGTALNTTAGTIRQTGRNLDV
                     AIEGDGYFVVQTQQGIRYTRAGNFTRDPSGQLTTANGDPVMGEKGPISLPPGEAHITP
                     DGEITVGYQSVDKFQVVRFDKPSRDLVKEGNTLLTLNDQTGITPQQVTGVRVVPGALE
                     GANVDTVSEMAAMIHHSREFESLQKAISMLMNDVGRKVGNDIGRL"
BASE COUNT          383 a          389 c          369 g          294 t
ORIGIN      
        1 attatggaat ccgcatcact gaaattgcgt ccccgcacga acgacctcaa ctccttgggt
       61 gaacatatat gaatattttt ctttggacgg ctggaatgag tgcgctggcg atcctggtga
      121 cctgcctggt cagtttgatt gtcatgcgaa aaattcgtca ggcaattcaa acggcagaag
      181 aaaaggtccg ggaaacagaa aacaaactgc tcagttccaa agaagaattg aacagtttgt
      241 ccaaacgcgt tgtggaacaa tcctcgcgca ttgcctggct cgaatcacgg gttcgcaata
      301 aaccagtccc ggcgggaacg cagtcacccg aacccgaacc ccaggctggc ccgaccaaat
      361 tgagtttgac ggaacgccga caccgggtcc tgtcactggc ccgtcgtgga tttgataccg
      421 atgcgatctc gtcgcttttg aataccccga acggagagat tgaacttatc ttgagtctga
      481 cgacccaggg cgccgggctc cgggtggaag gataattcca tccaaggttg aaggaatcag
      541 gcccagaaac gtcctcacgt tatgaaattt tgacaatcag gagaatcagt tatggaccac
      601 ggattataca ccgcatacct gggactacgg gcccgccaaa acgcccttga tgtcacagcc
      661 aacaacattg ccaatgcttc gacaccagga tttaagggcg actatatgct gtaccgatca
      721 atccagctat ctgcccagga gcaatcagcc gccccgacgg gcatcaatcc ccaggccatc
      781 actcaactcg acccgtcaca ggctgccgcg catacccgct ccaccggact ggtcaccgga
      841 acggcactca acaccacagc cggtaccatc cgtcaaaccg gaagaaattt agatgtcgcc
      901 atcgagggag acgggtactt tgtggtgcaa acccaacagg gaattcgata tacgcgggcc
      961 ggaaatttta ctcgtgatcc gagcgggcaa ctcaccacgg ccaacggaga cccagtgatg
     1021 ggcgaaaaag ggccaatttc gctaccaccc ggcgaggcac atattacgcc cgatggtgaa
     1081 attacggttg gataccagtc agttgataaa ttccaggttg tccggtttga taaacccagc
     1141 cgtgacctgg tgaaggaagg caataccctg ctgacgctca atgaccagac cgggattacc
     1201 ccgcaacagg tcaccggtgt gcgcgtcgta ccgggggcgc ttgaaggcgc taacgtggat
     1261 accgtctcag agatggcggc aatgatccat cattcgcgtg agtttgaatc gctccaaaaa
     1321 gcaatttcga tgttgatgaa cgatgtcgga cgcaaagtcg gaaacgacat cggaagactc
     1381 taataccagt ttgtagtcag tagcccgtag tcagtagttc actaagttta tttga
//
LOCUS       DAOQDO010001975         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_105904_length_1435_cov_2.842754, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001975 DAOQDO010000000
VERSION     DAOQDO010001975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105904_length_1435_cov_2.842754"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKZ53_24260"
     CDS             <1..187
                     /locus_tag="PKZ53_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43617.1"
                     /translation="PVAESVRRNVQPSEVGPVTVSTLDGGQFSLSPASPAGRWRHSIP
                     PVAPGATFSHLLRRLNS"
     gene            complement(264..920)
                     /locus_tag="PKZ53_24265"
     CDS             complement(264..920)
                     /locus_tag="PKZ53_24265"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNJ43618.1"
                     /translation="MTKPKYALDPKLAQLFQEASDWRLLSLLFECPRRHWQREITKLA
                     AQTTDPDLKEAAQAALQQASEGLYHSTFGPGGPVPGREISYHTMAQPGYLLSEVACYY
                     AAFGYQPDSREAPDHIAIEIGFFSYLRLKEAFALAAKDAEAAQITTETAEHFMTEHLS
                     VFGGQFSQSLVASEIPYLVAAGRSLLRRVGPRKSLPLYPASPTQPFQQDEECGFACGE
                     "
     gene            complement(917..>1435)
                     /locus_tag="PKZ53_24270"
     CDS             complement(917..>1435)
                     /locus_tag="PKZ53_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43619.1"
                     /translation="QVPTKIEANVPAPQMGEAGKPVEITIWKADWQAWTNGREDSIKS
                     IYPNAQPDHYPFEAQSLPKGSPEQQEMAKRYAPASAAGNRRVGPRDNPVEDLLAEGPG
                     TITPVARGARGLGVRTETGWQVVIVRKLPTGLSPQVRSQIAFAVWEGSHQEVGARKMR
                     TGWVPLQMKVQP"
BASE COUNT          304 a          403 c          398 g          330 t
ORIGIN      
        1 gccggtggca gagagcgtcc ggcgaaatgt ccagccatcg gaagtcggac ccgtgacagt
       61 ctcaaccctc gatggcggtc agttctctct ttcgccggct tcgccggctg gcaggtggcg
      121 gcattcgatt cccccggttg caccgggggc tactttctcc cacctgcttc gcaggctcaa
      181 ctcctgaatt tccagcttgt tccatagaac aaaacccaca cccggaaaaa atttgggtaa
      241 tgacaagacc ccgaaccccc aaattactcc ccacaggcaa acccgcattc ttcgtcctgt
      301 tgaaagggtt gggtgggtga tgccgggtag agtggcaaac tttttcgcgg gcccacccgc
      361 cgaagcaatg acctgcccgc agcgaccaaa tagggaattt cagaagcaac cagcgattgg
      421 gaaaattgcc cgccaaatac cgacaaatgt tcggtcataa aatgttcagc cgtttcagtt
      481 gtgatctgag cagcttcagc atctttcgcg gccagagcaa acgcttcttt aagtcgcaaa
      541 taggagaaaa aaccaatttc aatggcaata tggtcaggtg cctcacgtga atcaggttgg
      601 tatccaaagg cagcgtagta acaggccacc tccgagagca aataccctgg ctgagccatc
      661 gtgtgatagc tgatttcacg tcccggaacc ggtccgcccg gtccaaaggt cgaatgatag
      721 agtccctcgg aagcctgctg aagcgccgct tgagctgctt ctttcaggtc tgggtcggtc
      781 gtctgggcgg caagtttggt gatttcacgt tgccagtggc gacgcggaca ctcaaaaagg
      841 agactcagca gccgccaatc agaagcttcc tgaaataact gtgccagttt ggggtcaagc
      901 gcatatttgg gttttgtcat ggttgaacct tcatttgaag cggtacccag ccggttcgca
      961 tcttccgggc gccgacttcc tggtgactgc cttcccagac ggcaaaggca atctgggatc
     1021 gaacctgtgg ggacaggccc gtcgggagtt ttctgacaat gaccacctgc cagccggttt
     1081 cagttcggac accaagcccc cgggcacctc gggcgacagg tgtaatcgtg ccggggcctt
     1141 ccgccagcaa atcttccacc gggttgtcac gcgggccaac ccgccggttg ccagccgcgc
     1201 tggccggagc gtagcgtttg gccatttcct gttgttcagg tgagcctttt ggcagcgact
     1261 gggcttcaaa tggatagtga tctggctggg catttggata aatggattta atcgagtctt
     1321 cgcggccatt ggtccaggct tgccagtcag ctttccaaat tgtgatttca actggtttgc
     1381 cagcttcgcc catttgtggc gctggcacat tggcttcgat tttggtcggc acctg
//
LOCUS       DAOQDO010001976         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106036_length_1434_cov_2.993474, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001976 DAOQDO010000000
VERSION     DAOQDO010001976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106036_length_1434_cov_2.993474"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..960)
                     /locus_tag="PKZ53_24275"
     CDS             complement(163..960)
                     /locus_tag="PKZ53_24275"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNJ43620.1"
                     /translation="MISSLAGFLRLAAAYLKFNLSAQLAYRGAFISQVVAMFLNDIVW
                     VTFWWLFFTRFPVLRGWDVKDVITVWAIAAAGFGISHGICGNAFSLSSLIVQGQLDVW
                     MLYPRTLLPHLLLGRTNATAWGDALFGYVIYLVIVRPDLAHFLLFFLLSISAAMVFLG
                     FNILVGSLAFFLGNSEGLAEQLRFSLITFSTYPSVIFEGPVKIILFIVIPAMFINNLP
                     ITALRELSLLHASLSFLGGSLALGIGGSVFYYGLRRYESGNLMNMRG"
     gene            complement(1040..>1434)
                     /locus_tag="PKZ53_24280"
     CDS             complement(1040..>1434)
                     /locus_tag="PKZ53_24280"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNJ43621.1"
                     /translation="PISISLSGLAMFAVSIPLAFVLDFLGMFLIGLGAFWLEDTAGLT
                     LIYSRITMILGGLLIPIELFPDAIQPIVRILPFASMVYGPAHMFVAPGWSQLIDLIIK
                     QSLGIVVLGVIVSFVYQLALKRISAHGG"
BASE COUNT          454 a          363 c          334 g          283 t
ORIGIN      
        1 tgcttggtgc ttggtgcttg gtgcttggtg cttggtgctt ggtgcttggt gctttcgaaa
       61 gcattgactt ctagccactg ctacgcggcg aagaaatatc aacagaacct atccactaac
      121 cacattcttc attcttcact ctcaattctt cattcgactc agtcagccgc gcatattcat
      181 caaatttcct gactcgtagc ggcgcagccc ataataaaaa actgatccgc caatccccag
      241 ggccagcgac ccacctagaa acgaaaggct agcatgaagc agagatagtt cgcgcaaggc
      301 agtgatgggg aggttattga taaacatggc tggaatcacg ataaacaaaa tgatcttgac
      361 cggtccttca aaaataactg acgggtaggt actgaaggtg attaacgaga agcgcaactg
      421 ttcagccagt ccttctgagt ttccaaggaa aaaggcaaga cttccgacca gaatgttaaa
      481 tccgagaaaa accatggccg ccgaaatact caacagaaag aagagcagaa aatgagccag
      541 atctggccga acaatcacca gataaatgac atatccaaac agggcatcgc cccacgctgt
      601 cgcattcgtt cgtcccagca gcagatgcgg gagcaaggtg cgtggataca gcatccagac
      661 atcaagctga ccctggacaa tcagcgatga aagcgaaaac gcatttccac agatgccatg
      721 tgaaattcca aacccggcag ccgcaattgc ccagacggta atcacatctt ttacatccca
      781 gccccgcagg accggaaagc gcgtgaagaa cagccaccag aaggtaaccc acacaatgtc
      841 gttcaaaaac atggccacga cctgtgagat aaaggcgcca cggtaggcca gttgggcgct
      901 gagattaaat tttaagtaag cggctgccag ccgaagaaaa ccagcaagtg acgaaatcat
      961 aaatcgaatc agaggtggag tttatggttc agagttgaat gaaacccaaa tatttcaatt
     1021 agttcccaga tgtttgtctt tacccgccgt gggctgaaat ccgtttcagg gcaagctgat
     1081 acacaaaaga gacgataaca cccaggacca caatcccaag cgattgcttg atgatcagat
     1141 caattaactg tgaccatcca ggggcgacaa acatgtgcgc cgggccatac accatgctgg
     1201 caaacggtaa aatccgaaca attggctgaa tggcatctgg aaagagttca atcgggatca
     1261 acaacccgcc taaaatcatg gtgatccgcg agtaaatcag tgtcagcccg gcagtatctt
     1321 ccagccagaa agcgccaaga ccaatcagaa acatccccaa aaaatcgagt acaaatgcca
     1381 gaggaatcga aacggcaaac atcgccagcc ctgaaaggct gatcgaaatt ggtc
//
LOCUS       DAOQDO010001977         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106069_length_1434_cov_2.313270, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001977 DAOQDO010000000
VERSION     DAOQDO010001977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106069_length_1434_cov_2.313270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKZ53_24285"
     CDS             complement(<1..685)
                     /locus_tag="PKZ53_24285"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ43622.1"
                     /translation="MVGQWLWQKLGICTDRACRQKLVLYLDGQLSSQDSQKVHAHLNQ
                     CRICHSELETLTFATRLAAQIVLPTAPSAGRPTWKKPSAASTLTSPVRQPVSRWPVLV
                     PVSLALMLAVASGVLWRLTPKMTEPTWEVIRLAGVPHIGQTPLGKTGYLKTGQVLQTD
                     AQSRALINFNRLGQVEVDPNSVLRLVKPGSSEYRFSLIKGRIFATIQAPPRLFFIDTP
                     AGTAVDLGCA"
     gene            complement(691..1284)
                     /locus_tag="PKZ53_24290"
     CDS             complement(691..1284)
                     /locus_tag="PKZ53_24290"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ43623.1"
                     /translation="MTSTSHGPAPDLIHRSDEELVPLIVAGQEEVFSIVYDRQYGRIY
                     RLVFGMLGRHEGADDVTQEIFLRAFLKLAQFDGRAQFSTWLYRLAVNHCLNQVSTIQR
                     EDQMYESDQDLPELTNDLPSAESLILQHQIQHQVTQALLSLKPHYRIVIILRDIEGFR
                     YEDIAEQLNCSVGTLAARLKRARMLLARKLEHLKGMV"
BASE COUNT          361 a          354 c          381 g          338 t
ORIGIN      
        1 aagcacagcc aaggtcaacc gccgttccag ccggagtatc aatgaaaaag aggcgcggcg
       61 gggcctgaat tgtggcaaaa atacgccctt tgatcagtga aaagcgatat tcgctggacc
      121 cgggtttaac caatcgcaag accgaattcg ggtcaacttc aacctgtcca agccgattga
      181 aattgatcaa tgctcgtgat tgagcatcgg tttgaaggac ctgtcctgtt ttaaggtaac
      241 cagttttgcc cagcggcgtt tgcccgatgt gcggcactcc ggccagccga atcacctccc
      301 aggtgggttc tgtcattttg ggagtcagcc gccagagcac gccgctggcc acagccagca
      361 tcagtgccaa actgaccggc accagcacag gccatcggct gactggctga cgaactggac
      421 tcgtcagagt gcttgccgca gatggttttt tccacgttgg gcggcctgca gaaggtgctg
      481 tgggtaatac gatctgagct gccagtcgag tggcaaacgt aagggtttca agctctgagt
      541 ggcatatccg gcattgattc aggtgggcat ggactttctg gctgtcttga gaagacaact
      601 ggccatccag atacaacacc agtttctggc gacaggcgcg gtcagtacaa atacccagtt
      661 tctgccagag ccactgtcca accatggccg ttacaccatt ccttttaaat gttcgagttt
      721 ccgagccagc agcattcgtg cccgcttcag acgtgccgcc agcgttccaa ccgaacaatt
      781 gagttgttcc gcaatgtctt cataccgaaa cccttcgata tcgcgcaaaa taatgacaat
      841 tcgatagtgt ggtttgaggc tgagcagtgc ctgggtgacc tgatgctgaa tttgatgttg
      901 caaaatgagg gattcagccg aaggtaaatc gttcgtcagt tctggcagat cctggtcaga
      961 ctcgtacatt tgatcttccc gttggatagt ggaaacctgg tttaaacaat ggttgacagc
     1021 caaccggtag agccaggttg aaaactgtgc ccgcccgtca aactgcgcca gtttgagaaa
     1081 cgcacgtaga aaaatctcct gggtcacatc atcagcacct tcgtggcgtc ccagcattcc
     1141 aaaaaccagt cggtagatgc gtccatactg tcggtcatac acaatggaaa agacctcttc
     1201 ctgaccggca acaatgagag gcaccagttc ttcatcagag cggtgaataa gatctggcgc
     1261 tgggccgtga gaggtggagg tcacgatgga ttgggtaaac tgtggccctg gttgaattcc
     1321 aaaaacataa aaggttgact ggttttcagt gattgagatg gtgtttgtcg aattagacag
     1381 aagtagatca aactttatgt caaaatttgt tcagacgttc aaaaacaccg gggt
//
LOCUS       DAOQDO010001978         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106175_length_1433_cov_2.862119, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001978 DAOQDO010000000
VERSION     DAOQDO010001978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106175_length_1433_cov_2.862119"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1232)
                     /locus_tag="PKZ53_24295"
     CDS             complement(237..1232)
                     /locus_tag="PKZ53_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNJ43624.1"
                     /translation="MVAIIAGIGIGSVTIAPDSVVRIIASHVLPAGWVDVTGIRDVDV
                     AVVWLIRTPRVLVAVLVGAGLAIAGAQMQGLFQNPLASPDIIGTSSGGALGAVIALAT
                     GLATRSLWYMPILAFLGALVSLFVVYAISTQRGRTPVSTLLLAGVALNALIGAGTSFV
                     ITLKWVRWEVATGIIFWMMGGLDTRTWDHVWLALPCVLVGIGIALMFTRELDVLLLGE
                     EFALGLGIDVERAKRIILTGAALLTGTAVAVSGVVGFVGLIIPHIVRLVIGPRHRYLL
                     PASALTGASFLVVADILARTLQSPEEIRLGIITAVFGAPFFLYLLLKHQQAANSI"
     gene            complement(1129..>1433)
                     /locus_tag="PKZ53_24300"
     CDS             complement(1129..>1433)
                     /locus_tag="PKZ53_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43625.1"
                     /translation="GAEDIGLGAEDIGLGAEENGFHTLSPMSSAQRLQPNVFSPTSSA
                     QRLQPRFRTRNPMVLPHRHFYSGGGNHRWHWYRECNDCTRLGSADHRFARASGWVG"
BASE COUNT          395 a          395 c          382 g          261 t
ORIGIN      
        1 gcatttaaat gttagctaca gatgaatgtg gcaggtttga taacccagcc agtctaatca
       61 ggtaaataat tgaaatttag ctatttccag gggatttaag tcagaattga gaaaagcaag
      121 ctgagggaca ttcaggtgat aggcttttct cggattctga tacttgagca gacctggtca
      181 aaattaccat aaactgaagg taaaaatgac cgttcactgt ttcccgaaac cgatgattag
      241 attgaattgg ccgcctgttg gtgtttgagc aggaggtata gaaaaaatgg agccccgaac
      301 acagcggtga tgatgcccag gcgaatttct tccgggctct gcagggtccg tgccagaata
      361 tcggccacaa ccaggaacga ggccccggtc agggcgctgg ccgggagcaa atagcgatgg
      421 cgaggtccaa tcaccagccg cacaatgtgc ggaatgatca accctacaaa cccaacgacg
      481 ccgctgacag ccacggcggt cccggtcagg agggcggcgc cagtcagaat gatgcgtttt
      541 gcgcgttcga catcaatgcc aagcccaagg gcaaattctt caccaagcag caacacatca
      601 agttcacgtg taaacatcag ggcgataccg atcccgacca gtacacaggg caatgcgagc
      661 cagacgtggt cccaggtgcg ggtatcgagc ccacccatca tccaaaaaat aatgccggtg
      721 gcgacttccc agcggaccca cttcaaggtg atgacaaacg aggttccggc accaatcaag
      781 gcattgagtg caaccccagc cagtaaaagc gttgaaactg gcgtgcggcc tcgttgagtc
      841 gaaatggcat acacgacaaa gagggaaacc agtgcaccaa gaaacgccag aatcggcata
      901 taccacagcg agcgcgtggc caaccccgtc gccagcgcaa tgacggcccc cagcgctcca
      961 cctgaactgg tgccgatgat atcaggcgaa gccagcgggt tttgaaacaa tccctgcatt
     1021 tgagccccgg caatcgccag tccagcacca accagaaccg ccaccaggac gcgaggtgtc
     1081 cgaatcaacc agaccacggc cacatctacg tctcgaattc cggttacatc aacccaccca
     1141 gccggaagca cgtgcgaagc gatgatccgc actaccgagt ctggtgcaat cgttacactc
     1201 ccgataccaa tgccagcgat gattgccacc accagaataa aaatggcgat ggggaagaac
     1261 catcgggttc cgggttctaa agcggggctg aagacgttgg gctgaagacg ttgggctgaa
     1321 gacgttgggc tgaagacgtt gggctgaaga cattgggctc agggtatgaa acccgttttc
     1381 ttcagcccca agcccgatgt cttcagcccc aagcccgatg tcttcagccc caa
//
LOCUS       DAOQDO010001979         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106287_length_1432_cov_3.053740, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001979 DAOQDO010000000
VERSION     DAOQDO010001979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106287_length_1432_cov_3.053740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..404
                     /locus_tag="PKZ53_24305"
     CDS             231..404
                     /locus_tag="PKZ53_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43626.1"
                     /translation="MKSWVRATGPDVFQELSLREPSDKVSQPGYLRYDLPSVRMEATT
                     VQTIQKMQTTSCK"
     gene            485..1096
                     /locus_tag="PKZ53_24310"
     CDS             485..1096
                     /locus_tag="PKZ53_24310"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HNJ43627.1"
                     /translation="MSKVLARFAPILLLSSLFLIVLAIPVSAQEERHQDHEELRALLK
                     TATEALNARNFDALAPHLYRQFSITTVDQKEFSDLNSFKSHYAALFSGDKPVLKDISL
                     KPVADKLTVFAGDNTGVCWGTSTDTFTFTDGETRTMTSRWTATVVKDEGKWKIMSVHF
                     GANFMDNPVTQTFQGMLYKIGGGALAVGIVLGGLIGWVLGRRK"
     gene            1205..>1432
                     /locus_tag="PKZ53_24315"
     CDS             1205..>1432
                     /locus_tag="PKZ53_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HNJ43628.1"
                     /translation="MLDLILHNGLIFDGLGSAPQRASLAVEQGRVVAISDRIDTPARE
                     AIDAAGMWITPGFLDIHTHYDLEVEIAPALLE"
BASE COUNT          332 a          368 c          367 g          365 t
ORIGIN      
        1 caatcgtttc ccgtgatttg cacgggggtc tggtcaagtg aagcttgagt cagtccacca
       61 atatcggctc tccgttagga tagcgtcgtg agtacacgac tcaagcttgg cccttgaggg
      121 ccgagtctct tgacaagagc ctccaacagc aaagaaaacc gggggattat ttttaggatt
      181 tgacgcgaaa caaagtcaac agaatcggtg aaattgattg cgtcgggtga atgaagtcgt
      241 gggtacgagc aactggtccg gatgtgttcc aggaactttc cttgcgcgaa ccttccgaca
      301 aggtttcaca acccggctat ctccgctatg atttgccctc ggttcgtatg gaagccacca
      361 ccgttcaaac gattcaaaaa atgcaaacta cttcctgcaa atagttgatt ttgcagatga
      421 tcgttcattg ttcaaccttg aacaatttgt attgaacgcc ctttttcaac ttttcggagg
      481 acctatgtcg aaagttcttg cccgctttgc cccgatcctg ctgctgtcca gtttattttt
      541 aatcgtgctg gccattccag tgagtgctca ggaagaacgc caccaggatc acgaggaact
      601 tcgtgccctg ttaaaaacgg caaccgaagc tctcaatgcg cgcaatttcg atgcactggc
      661 accccattta taccggcagt tttcaatcac aactgtggat caaaaagaat tttccgacct
      721 caattccttc aaatcgcatt acgccgcctt gttttccggc gacaaacccg tgttgaaaga
      781 catctcgctc aaacccgtgg ctgacaaatt gaccgtcttt gccggagaca acactggcgt
      841 gtgctggggg acatcaaccg atacctttac ctttactgac ggtgaaaccc gaaccatgac
      901 cagccgctgg acggcgacgg ttgttaaaga tgaggggaaa tggaaaatca tgagcgtcca
      961 ttttggggcg aattttatgg acaatccggt cacccagacg ttccagggaa tgctctacaa
     1021 aatcggcggc ggcgcactgg cagtcggaat tgtacttggc ggactcatcg gatgggttct
     1081 tggccgacga aaataatttc ggagtgcggc ggagtgcggc ggcttgacgc cgctttgtca
     1141 gagtgcggcg gcttgacgcc gctttttttg gactgcggcg gcttgacgcc gctttgtcat
     1201 tcccatgctc gatctcatcc ttcacaacgg tctgattttt gatggcctcg ggtctgcgcc
     1261 gcaacgtgcc agcctcgcgg ttgaacaagg ccgggtggtt gcaatttcag accgaattga
     1321 taccccagcc cgtgaggcga ttgatgccgc tggaatgtgg atcacgcctg gatttcttga
     1381 tattcacacc cactatgatc tggaagttga aattgcaccg gcgctgcttg aa
//
LOCUS       DAOQDO010001980         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106434_length_1431_cov_3.209302, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001980 DAOQDO010000000
VERSION     DAOQDO010001980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106434_length_1431_cov_3.209302"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKZ53_24320"
     CDS             complement(<1..922)
                     /locus_tag="PKZ53_24320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43629.1"
                     /translation="MKKYDLIVIGSGPAGEKAAARAAYFGFQVAVIEKAPRVGGAGIL
                     TGTLPSKTLKETALYFSGKLDKGLYGVDRQFSRETSIGDFMFRKDFVTTSESNQMHNN
                     LLRHKIDVYFGLGQFQDSHTVHIQKADGEELITADYILVSTGSYPYHPPHIPFDGKRV
                     HDSDTILTLDRFPHTLAVIGAGVIGCEYATIFSTVGTKVFLVNSTDAILPFLDKAISQ
                     ALVDKMNKDGVEILFNTSFDQIDVPVDDSQPIRIPLKSGRTLEVDMFLYAAGRCGNIR
                     GLNCDRAGIHLGPRETILVDTDYRTNVPHIF"
BASE COUNT          378 a          317 c          371 g          365 t
ORIGIN      
        1 caaaaatatg gggaacattg gtccgatagt cggtgtcaac caggatagtt tcacgggggc
       61 caaggtgaat gcctgcgcgg tcacagttga gtccacgaat attgccgcac cgcccggcgg
      121 catacaggaa catatcaact tcgagtgtcc ggcctgattt gagcgggatg cggatgggtt
      181 gcgagtcatc aaccgggacg tcaatctggt caaatgaagt attgaacagg atttcaactc
      241 catctttgtt cattttatca accagcgcct gggaaatcgc tttatcgagg aagggaagaa
      301 tcgcatcggt tgagttgacg agaaacactt tggtgccaac cgttgaaaaa atcgtggcgt
      361 attcacagcc gatgaccccg gcaccaatca cggccagcgt gtgtggaaag cgatcaagcg
      421 tcaaaatcgt gtctgagtcg tggacccgct tgccatcaaa gggaatatgt ggagggtgat
      481 atgggtagga accagttgaa accagaatat agtctgcggt gatgagttcc tcaccatcag
      541 ctttttggat gtgaactgtg tgtgaatctt gaaattgacc aaggccaaag tacacatcaa
      601 ttttatgccg gagcaaattg ttgtgcatct ggttgctttc actggtggtg acaaagtcct
      661 tgcggaacat gaaatctccg attgaggttt cacgtgaaaa ttggcggtca acgccataga
      721 gacctttgtc aagttttcca gaaaaataga gtgcggtttc cttcagcgtt ttcgatggca
      781 aggtgccagt caagataccg gcgccgccaa cccgtggcgc tttttcaatc accgcgacct
      841 gaaacccgaa ataggctgcc cgggccgctg ctttttcacc ggctggaccg gatccgatca
      901 cgattaagtc atattttttc acggctggag actctccacg aagcaggaat agacattaca
      961 gaaggggaca cctgtgctcg accaacgttt ggggagttgg tgcccatact ggctgtcagg
     1021 cggtctggtg tcaaatagtt tcagaaagaa agcgtgagcc cggttgattg attttcatca
     1081 gttgaaaaat tcgagttaat tgcaactggg aaaagcaatt acccgtagag tgcgactgat
     1141 ttccaccctg ggtgtcaacc acctgattta ctctccaaag gaggattccc aacgccagga
     1201 taaaaaagcg ggttgattgt tcgaaattgg accaattttt caggggctcg gcccttgtcg
     1261 ttcatcagtg ggtgcgttag attaacattt gccgttcaac ttcgaaaaag tcttgattca
     1321 gaccaggtaa attaaaattt taatttacct caccccgtac ggctggcgtt gggctatgat
     1381 ttcaaaaatg cactatttaa aagaagccgt aactgagcct ttaaatattt g
//
LOCUS       DAOQDO010001981         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106442_length_1431_cov_2.999273, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001981 DAOQDO010000000
VERSION     DAOQDO010001981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106442_length_1431_cov_2.999273"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1431
                     /locus_tag="PKZ53_24325"
     CDS             191..>1431
                     /locus_tag="PKZ53_24325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ43630.1"
                     /translation="MTTVLHILHQISSGGGSRAAIQVASESSRQSPVLCHRLVSLTPA
                     NPQALKLAQSAGLTVLNAPSDETLWAEIAAADVVHVHFWNTPELYQVLSSELPPCRMV
                     VWVHIAGDSPPQVIIPELIEYADFVVACSPYTARHPVFQSALADQKTTMILAGCDPHR
                     LSGFSRQPQSTFTIGYIGTVDFIKMHPDFIRLHDEIDLPEVKVVIVGTGASFKRVQSQ
                     IQQAQHPERFVLSGQIEDVTSVFEQLDVFGYPLCPENYAAAELVLQEAMLAGVPPVIF
                     PYGGARDLVVDGQTGLIVASEADYPKAIERLYFDRELRQQLGTNAQLYAHQFFGADKT
                     ATQFNRLYKQLIERPKRERPALDTLLERHQPAAWRALKAQFPGAVSFLSSLGTSAPAF
                     QTSFWSSDVNEQLAAESEIAT"
BASE COUNT          343 a          391 c          351 g          346 t
ORIGIN      
        1 gccagccttt gaaatggggt ttccgcttcg acatccagcc gaaatcacac gccatattca
       61 agccgatgac tttaccgagc aaaccatttt tagccgcaag gtgggaaagg tatttgaagc
      121 ttttaaatct cggaaaaaac aatagcgttt atcaaccctg cgaaccgcgc gaaatgccaa
      181 tttttcattc atgacgacag ttctgcacat cctgcaccaa atatcgtccg gaggaggcag
      241 ccgggcagcc atccaggttg ccagtgaatc atccagacag tcaccggtgt tgtgtcatcg
      301 gctggtctcg ctgacgccag ccaatcctca ggcattgaag cttgcccaat cagccgggct
      361 gaccgtgctc aatgcacctt cggatgaaac actctgggct gaaattgccg ctgccgatgt
      421 ggttcacgtc catttctgga acacaccgga actctatcag gtgctctcat cagagttacc
      481 accgtgccga atggtcgttt gggttcatat cgccggtgat agcccgccac aggtcattat
      541 cccggaactg attgaatatg ctgactttgt cgttgcctgt agcccataca ccgccagaca
      601 tccggtcttt caatcggctc tggctgatca aaaaacgacc atgatcttgg ccggatgtga
      661 tccgcatcgg ctgtcaggat tttcccgcca accgcagtca acgttcacca tcggatatat
      721 cgggacggtg gatttcatca aaatgcaccc agactttatc cggctgcacg atgaaattga
      781 cctgccagaa gtgaaagttg tcatcgtcgg tactggtgcc agtttcaaac gggttcaatc
      841 ccaaattcag caagcccaac atccagaacg atttgtcctg tcaggtcaga ttgaagatgt
      901 cacatcggtg tttgaacagt tggatgtgtt tggctacccg ttgtgtcctg aaaattatgc
      961 ggcagcagaa ctggtgctcc aggaagccat gctggcgggt gtcccaccgg ttatttttcc
     1021 gtatggcggt gctcgtgatt tggttgtgga tggccagacc ggactgattg tggcgtctga
     1081 agccgattat ccgaaagcca ttgagcggct ctattttgat cgagaactcc ggcagcaact
     1141 tggtacaaat gcacagttgt atgcacatca attctttggc gccgacaaaa cagccactca
     1201 gttcaatcgc ctttacaaac agttgataga acgccccaaa cgagagcgtc cagcgctgga
     1261 cacgctcctc gaacgtcacc agccagccgc gtggcgggcg cttaaagccc aattccctgg
     1321 cgcagtttct tttctctcgt cacttggcac cagtgcaccc gcgttccaaa ccagtttctg
     1381 gtcgtcagat gtgaatgaac aactggcagc ggaatcagaa atcgcaacca g
//
LOCUS       DAOQDO010001982         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106513_length_1431_cov_1.927326, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001982 DAOQDO010000000
VERSION     DAOQDO010001982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106513_length_1431_cov_1.927326"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKZ53_24330"
     CDS             complement(<1..391)
                     /locus_tag="PKZ53_24330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43631.1"
                     /translation="MDWEHWWHDVLEDIETWCRNKSPIWRAILLLYFGYAGVHSVFDP
                     EYQNFFSGITFGIHELGHVMFSFAGLFISVLGGSLAQVAAPLIVIGGFLHQRDYFAVT
                     IGSSWLSLSLSNLATYVGDARAQSLPLL"
     gene            526..1338
                     /locus_tag="PKZ53_24335"
     CDS             526..1338
                     /locus_tag="PKZ53_24335"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNJ43632.1"
                     /translation="MKKQFSHFAFALVLVIAASAVAMAQNFQKSYPLSAGGSISVKNV
                     SGDIKINGYGGETVQVTATKEGRDADRVEIEDLSSGNRVELRVKYPQNCNCDASVNFE
                     VQVPKSTKFQFDKIATASGDISLEEVTGDIDAKSASGDVRVVNSSGDIDVATASGDVL
                     VDDAEGLVSAKTASGDVKVNITNLSNASGKMEFASASGDVDVRVPGNLGAQVDMATVS
                     GDVHSDFSLNVEKREYGPGATARGSIGNGGMRISIKSASGDVSLRKSGGRAN"
BASE COUNT          390 a          358 c          376 g          307 t
ORIGIN      
        1 ccagcaacgg caggctttga gcgcgggcgt ccccgacata ggtggccaga ttggaaaggc
       61 tgagcgagag ccagctactg ccaattgtca cggcaaaata atcacgctgg tggagaaatc
      121 ccccgatgac gatgagtggg gccgcaacct gagccagact cccgcccaaa accgaaatga
      181 acaacccggc aaagctaaac atgacgtggc cgagttcgtg aatgccaaag gtaatgcctg
      241 aaaagaaatt ttgatattcc ggatcaaaca ccgaatggac accggcatat ccgaaataga
      301 gcagcaatat tgcccgccag atcggagatt tgttccgaca ccaggtttca atatcctcaa
      361 gtacatcgtg ccaccagtgt tcccaatcca tagcaccacc ttctgaattt tatctgagaa
      421 ttcgtggaaa ttttttctgc aacttttttt gattgtctca cacacactcg ggcaacaggt
      481 caagagaatc aaaacacatt tgaaaggaat ccccgaggag acaatatgaa aaaacaattt
      541 tcccatttcg cattcgcact tgttttggtg atcgcagctt ctgcggtggc gatggctcaa
      601 aacttccaaa aatcctaccc actgtcagcc ggtggcagca tcagcgtcaa aaatgtttca
      661 ggtgacatca aaatcaatgg ctatggtggt gaaacggttc aggtgacagc caccaaagaa
      721 ggtcgcgacg ctgaccgcgt tgaaattgaa gacctgagca gcggcaaccg ggtcgaactc
      781 cgggtcaaat atccccaaaa ctgcaactgt gacgccagcg tcaactttga agtccaggtt
      841 cccaaatcaa ccaaattcca gttcgacaaa attgccaccg ccagcggtga tatttccctg
      901 gaagaagtca cgggcgatat tgacgccaag tcggccagcg gtgatgtgcg agtggtcaat
      961 tcttcaggtg acattgacgt tgcgacagcc agcggcgatg tgctggtgga tgatgccgaa
     1021 gggctggtca gcgccaaaac tgccagcggt gatgtcaaag tcaacattac aaatttgtcc
     1081 aacgcgagcg gaaaaatgga atttgcctca gccagcggcg atgttgatgt ccgtgtccca
     1141 ggaaatctgg gcgctcaggt agatatggca accgtgagcg gcgatgtgca ttcagatttc
     1201 tcgctcaacg ttgaaaagcg cgaatacggc cccggcgcca cggctcgtgg cagcattggc
     1261 aatggcggaa tgcggatttc aatcaagtca gcctcgggtg atgtttcgct gcgcaaatca
     1321 ggcgggcgag ccaattaaga catcgggctg aagaagtcgg gctgaagact tcgggcttgg
     1381 ggctgaagaa gtcgggttga agaactggtt ttatttcatc cttcatcctt c
//
LOCUS       DAOQDO010001983         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106580_length_1430_cov_2.810909, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001983 DAOQDO010000000
VERSION     DAOQDO010001983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106580_length_1430_cov_2.810909"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKZ53_24340"
     CDS             <1..678
                     /locus_tag="PKZ53_24340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ43633.1"
                     /translation="PLNGILGMSELVLDAYDTFSREEILEMVRDIHTSARRLHRLVEN
                     FWVYAKLETLCTDPGHLELIRKQAQTYSAKSIIEKVAVTKARDFSRIPDLHLTVADEV
                     IRISDSHLAKVVEELLENAFKYSIPGTPVIVTGTTGPETYLLRVTEQGRGMTANQIAD
                     IGAYMQFERTIYEQQGAGLGLAIVQRIVELNEGRLHIESVPDQQTTVEVEIPKLQLTF
                     PNTIEYV"
     gene            826..1401
                     /locus_tag="PKZ53_24345"
     CDS             826..1401
                     /locus_tag="PKZ53_24345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43634.1"
                     /translation="MRVNESELPTWSQLPEPVQVQFTAANISQPVFDSQLNRRRADCL
                     NFYAALTVHGLWECLFIEVPSREVGLNSILFRPQPDPRTLLERIKSNPAFTNLKTTIR
                     GRINSLLHPEEFNYSENIPWKASLHFTFNADGLAFVHFDYYNAHAASVWPVIGHAGYE
                     YPLKFVMSEFSTAKDIQLCLNERFGRNVWQP"
BASE COUNT          371 a          369 c          335 g          355 t
ORIGIN      
        1 ccgctcaatg ggatcttagg catgtcagaa ctggtactgg atgcctacga tacctttagc
       61 cgtgaagaaa ttctggagat ggtgcgtgac attcatacct cagccaggcg gctgcaccgg
      121 ctggtcgaga atttttgggt gtatgccaaa cttgaaacgc tctgcaccga tccaggtcac
      181 cttgaactga tccgaaaaca agcccaaacc tattccgcca agagcattat cgagaaagtt
      241 gctgtcacca aagcccgcga ttttagtcga atccctgacc tgcatctgac tgtggctgac
      301 gaagtcatcc gaatatcaga ttcccacctg gcaaaagtgg tcgaagaact ccttgaaaac
      361 gcttttaaat attctatccc aggcacaccg gtgattgtca ctggaacgac tggcccagag
      421 acctatcttc tgagggtgac ggagcagggc cgtggaatga cggccaatca aattgcagat
      481 attggggcat atatgcaatt tgagcgaacg atctatgagc agcagggcgc cgggttagga
      541 ctggcaattg tacagcggat tgttgaactc aacgaaggcc gcctccacat tgaaagcgtt
      601 ccagaccagc aaaccacagt cgaggtcgag atccccaaac tccagctcac atttcccaat
      661 acaattgagt atgtgtgagg aatgagcaaa accgtaaaga agctgagcct tgtatttcag
      721 cttggtttct cttaattcgt aagctctttg tttttgatag gtttgcttgc ttatcaattg
      781 caactccacg ctcttttccc gttcgctctt tcaccccaaa aacgaatgcg agtcaatgaa
      841 tcagaactcc caacctggag tcaattgcct gagccagtac aggttcagtt cacggctgcc
      901 aacatttccc agccagtctt tgattcccaa ctcaaccgga ggcgagctga ttgcctgaat
      961 ttttatgccg cactgacggt acatgggttg tgggagtgtc tgtttatcga ggtcccaagt
     1021 cgtgaagtcg ggttaaattc gattttattc cgaccgcaac cagacccgcg cacccttttg
     1081 gagcggatca aatccaaccc ggcgtttacc aatttaaaaa cgaccatccg tggacgaatc
     1141 aatagcctgc ttcacccgga agagtttaac tactcagaga atattccctg gaaagcctca
     1201 cttcacttca cgttcaatgc tgacggtctg gcatttgtcc attttgacta ctacaacgca
     1261 cacgctgctt ccgtctggcc cgtgatcggc catgccgggt acgaatatcc gcttaaattt
     1321 gtgatgtcag aattctcgac ggctaaagac atccagctct gcttgaatga acgctttggg
     1381 cgaaatgtct ggcaaccctg acaaagccag gatgacagaa gtgacagtgg
//
LOCUS       DAOQDO010001984         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106726_length_1429_cov_4.021106, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001984 DAOQDO010000000
VERSION     DAOQDO010001984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106726_length_1429_cov_4.021106"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..893)
                     /locus_tag="PKZ53_24350"
     CDS             complement(42..893)
                     /locus_tag="PKZ53_24350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ43635.1"
                     /translation="MAKNLRKAVSSERLAELAAIAPKISDVPVKTFGRRVLEQLRYVD
                     ITLLRPNPFQPRKTFSDEQLADLTTSITEQGIIQPVVGKANQDGTFTLIAGHRRWLAA
                     QKAGLSVIPMIVREASDEELQLLALIENIQREDLHTIDKAQALIDIADQFSTQEEAAQ
                     AVGMKRSSFANWLCVRELSPEVLDICRQIPDFSLKSLQRLTALPPNRRLMVARQMQTM
                     ATKTTQPEETKPVLSSPNLMTFHFDHPTHRYHCRIEVRARTRKHQMSRQEVREFLQLA
                     LESLNEE"
     gene            complement(886..>1429)
                     /locus_tag="PKZ53_24355"
     CDS             complement(886..>1429)
                     /locus_tag="PKZ53_24355"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ43636.1"
                     /translation="LTEDELTLHRQQDQARLVGYMDALRDRYDLILVDCSPKISEIVL
                     QVLIAVDALLVPVQTEAKAVAAFAEVQHEIARTQARRRNMRFPPIKVLGVVPTLFSPR
                     LVLHRHHLQELTSDICASFQYAALTPIRDYVAVSEAGTQGMPLKAYAPECQANQDVAA
                     VADEVLRFCGLKAIREVANG"
BASE COUNT          316 a          362 c          387 g          364 t
ORIGIN      
        1 cattctcaat tcttcattct caattcttca ttctcaattc ttcattcttc attcaaagac
       61 tcaagagcga gttgcaggaa ttcacgcact tcctgccggg acatctggtg tttgcgcgtt
      121 cgggcacgga cttcgatccg gcagtgatag cgatgggttg ggtgatcaaa gtgaaatgtc
      181 atcaggttgg gactggatag aactggtttg gtttcttcgg gttgagttgt ttttgtggcc
      241 attgtctgca tctgacgggc caccatcagc cgccgattgg gtggtaatgc ggtcagtcgc
      301 tgtagtgatt tgagtgagaa atcagggatt tgacggcata tatcgagcac ctcggggctc
      361 aattcacgca cacacagcca gttggcaaaa ctggaacgtt tcatcccgac ggcctgagcc
      421 gcttcttctt gcgtggagaa ctggtcggca atgtcaatca gcgcctgggc tttatcaatc
      481 gtgtgcaggt cttcacgctg gatattttca atcagggcca gcagttggag ttcttcgtca
      541 ctggcttccc gcacaatcat cgggatgacc gacagtccgg ctttttgagc cgccagccac
      601 cggcgatgcc cggcaatgag cgtaaacgtt ccgtcctgat tggcttttcc aaccactggc
      661 tgaataatac cttgttcggt aatgctggtg gtaaggtcag ctaactgttc atctgaaaag
      721 gtttttcgag gctggaaggg atttggtcgc agtaaggtga tgtctacata acggagctgt
      781 tccagcaccc ggcggccaaa ggttttgact ggaacatcac tgatttttgg agcaatcgcc
      841 gccagttcgg ccagtcgttc gctgctcact gccttgcgca gatttttagc cattggcgac
      901 ctcccggatg gcttttaatc cgcaaaaccg caggacttca tctgccacag ccgccacatc
      961 ctggttagcc tgacattcgg gggcataggc tttgagcggc attccctggg ttccggcttc
     1021 ggaaacggcg acataatcac gaattggtgt cagggcagca tactgaaacg aggcacaaat
     1081 atcagaggtt aattcctgca agtgatggcg atgcaatacc aggcgcgggc taaacagcgt
     1141 aggcacaacc cccagaactt tgatcggtgg aaagcgcatg ttgcgtctgc gagcctgagt
     1201 ccgggcaatt tcgtgttgaa cttcggcaaa cgctgccacg gctttggctt cggtctgaac
     1261 aggaacgagc agggcatcaa ctgcaattaa cacctgtagg acaatctctg aaatcttggg
     1321 cgagcaatcc accaagatca ggtcatagcg gtcacggagc gcatccatgt acccgaccag
     1381 tcgagcctga tcctgttgac gatgcagcgt cagttcatct tcggtcaat
//
LOCUS       DAOQDO010001985         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_106743_length_1429_cov_2.889374, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001985 DAOQDO010000000
VERSION     DAOQDO010001985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106743_length_1429_cov_2.889374"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1429)
                     /locus_tag="PKZ53_24360"
     CDS             complement(<1..>1429)
                     /locus_tag="PKZ53_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ43637.1"
                     /translation="ILQFTATQEDTAFIATSQRLNLITTELQEGVMKTRMQPIGNIWS
                     KFPRIVRDLALACGKRVQIDMEGKETELDRTIIEAIKDPLTHIVRNSIDHGIELPEVR
                     ESRGKSPEGRLFLRAFHEGGQVNIEISDDGAGIDPERIKARAIQRGMFPPEQANRLTD
                     RELINLIFAPGFSTAEKVTNVSGRGVGMDVVKTNIEKIGGTLDVINRPGEGTTLKIKI
                     PLTLAIIPALMVTTGGDRYAIPQVSLLELVRLEGAQAHNGIETIQGTPVYRLRGNLLP
                     LVYLNRELQVQDLSAPTETEIINIVVVQADDRQFGMVVDEINDTEEIVVKPLGKQLKG
                     LATFAGATILGDGRVALILDVLGIAQRASVVSELRDRAIAEKTLQYQESSGDRQTLLL
                     FQLGETDRLAIPLSMVARLEEFTGTAVERAGGQEVVQYRQQIMPLIRLSKILGRPTSR
                     QAGADPLQVIVYSEHGHSVGLVVDQ"
BASE COUNT          319 a          402 c          366 g          342 t
ORIGIN      
        1 atttgatcaa ccacaagccc aacgctatgg ccatgttccg aatagacaat cacctgcagc
       61 gggtcggctc cggcctgccg gctggtgggt cttcccaaaa ttttagataa ccgaatcaat
      121 ggcatgattt gctgacgata ttggacgact tcctggccgc cagcgcgctc gaccgccgtg
      181 cccgtgaatt cttccagccg ggcaaccatt gacagcggaa tggccaggcg atcagtttca
      241 ccaagctgga acagaagcag ggtttgccga tcacccgagg actcctgata ttggagggtt
      301 ttttcagcaa tcgcccggtc tcgtaattcc gagaccacac tggcacgttg ggcaatacca
      361 agaacatcca ggatgagcgc cacccggcca tcgcccagaa tggtggcacc ggcaaaggtg
      421 gccagccctt tcaactgttt gcccagcggc ttgaccacga tttcttcggt atcattgatt
      481 tcatcaacca ccatgccaaa ctgccggtca tcggcctgaa ccaccacaat attgataatt
      541 tcggtttcag ttggtgccga aagatcctga acctgtaatt cgcgattgag atagaccagg
      601 ggcaacagat tgccgcgcaa ccggtagacg ggcgttccct ggatggtttc aatgccgttg
      661 tgggcctggg ccccttcgag acgaaccagt tcgagcaggc tgacctgggg aatcgcatag
      721 cggtcaccgc cggtggtcac catcagggcc ggaataatcg ccagcgtaag tggaatctta
      781 attttcagcg ttgtgccttc gcccggacgg ttgatgacat caagcgttcc accgattttt
      841 tcgatgttgg ttttcaccac atccatgccc accccgcgtc cagacacatt ggtcactttt
      901 tcagcggtcg aaaacccagg ggcaaaaatc aggttgatca attcgcggtc tgtcagccga
      961 tttgcttgct caggaggaaa catcccacgt tggattgccc gcgccttaat gcgttccggg
     1021 tcaatgcctg cgccatcatc tgaaatttca atattgacct gaccaccttc gtgaaacgcg
     1081 cgcaggaaca gtcgtccttc cggagacttg cctctggact cgcgaacttc aggcagctcg
     1141 atgccatgat caattgagtt gcggacgata tgtgtcagcg ggtctttgat ggcttcaata
     1201 atggtccgat ccaattcggt ctctttgccc tccatatcaa tctggacacg tttgccgcaa
     1261 gccagtgcca ggtcgcgaac aatgcgcgga aatttactcc agatattgcc gataggctgc
     1321 atccgggtct tcatcacccc ttcctgtaat tcggtggtaa tcaagttgag tcgctgcgat
     1381 gtggcaataa atgcagtgtc ttcctgcgtg gccgtaaatt gcagaatct
//
LOCUS       DAOQDO010001986         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107072_length_1427_cov_2.501458, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001986 DAOQDO010000000
VERSION     DAOQDO010001986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107072_length_1427_cov_2.501458"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1427)
                     /locus_tag="PKZ53_24365"
     CDS             complement(<1..>1427)
                     /locus_tag="PKZ53_24365"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNJ43638.1"
                     /translation="IGDDSRANEAASLGVVAAFLRHKEELQDSILIPVDAHPELFGTR
                     DRQRPGENQRARCDLVRIRFRSRRFEATFIEVKYRSTLPTPNSQELMNRIVDQIEVTE
                     QVFKDLFFRRNPERLDHILQRSRLVAILRFYLRRSWRYGLIRNEARLHELEEGIGKLE
                     AGIPDVKIERRGYIVNLFGHPQKPVTLKECQIYFLCAQDLAGTDLRVASNAQSVPAQP
                     KLFDMTPVPLVTARRSVPETDSDQTEAHCAATSPELVSFPISPLVSDLAQEQIEASDE
                     PGRPTTPLPPLATRPLIPKETQLETPENRIPPDPVVVELGEIVGDQDTLQWKASVKGS
                     PHLFILGIPGQGKSVTVTRLLTELAKQGLPALVVDFHGQFGEPHSPYGQIAAPTVFDA
                     SRGLPFSPFEIDLETLRGTAWKTNCFEVAEIIQYVCELGDIQRDVVYEALIQCYLSVG
                     IGSGTPERLPRIEELAKTIEVCENS"
BASE COUNT          343 a          372 c          380 g          332 t
ORIGIN      
        1 ggctgttctc acacacttcg atggttttag ccagttcttc aatccgtggt agccgctcag
       61 gagtgccaga tccgatccca acgctcagat agcactggat cagggcttca tagacaacat
      121 cgcgctggat gtcgcccagt tcacagacat attgaataat ttcagcgacc tcaaagcagt
      181 ttgttttcca ggctgttcca cgcagggttt ccagatcgat ttcaaacgga gaaaaaggca
      241 atccccgact ggcatcaaac acagtcggcg ccgcaatttg gccataggga gaatgtggct
      301 caccaaactg tccatgaaaa tcaaccacca gtgccggcaa cccctgtttg gcgagttcgg
      361 ttaacagccg ggtcaccgtc actgatttcc cctgacccgg aatgccaaga ataaataaat
      421 ggggactccc tttgacactg gccttccact gcaaggtgtc ctgatcacca acgatttccc
      481 cgagttcaac caccaccggg tcaggtggaa tgcggttttc gggtgtttca agttgggtct
      541 cttttggaat cagcggtcga gtcgccagtg gcggcaatgg agtggtaggg cggccaggct
      601 catcactggc ctcaatctgt tcttgggcca gatcggaaac cagtggggag atgggaaatg
      661 acaccaattc aggtgatgtc gccgcacagt gagcttcggt ctgatccgaa tctgtctccg
      721 gaacgctccg tctggccgta accagcggaa ccggtgtcat atcaaagagc tttggctggg
      781 cagggactga ttgggcattt gaagccaccc tgaggtcagt cccagccaga tcctgggcgc
      841 ataaaaagta aatctgacac tccttgaggg taaccggttt ttgaggatgt ccaaaaagat
      901 tgacgatata gcctcgtcgt tcaattttga catcgggaat accggcttcg agttttccaa
      961 taccttcttc aagttcatgg aggcgtgcct cattgcgaat gagcccatag cgccaggacc
     1021 ggcgcaagta aaaccggaga atggcaacca gccgtgaccg ttgaaggata tggtcgagtc
     1081 gttccggatt ccgccggaaa aacagatcct taaagacctg ttccgtgact tcaatctgat
     1141 ccacaatccg gttcatgagt tcctgggaat tgggtgtggg cagggttgat cggtatttta
     1201 cttcgataaa tgtggcttca aaccgacggc ttcggaagcg aattcgaacc aggtcacacc
     1261 gtgcccgctg gttctcacca ggacgttgcc gatcccgagt gccaaataat tctggatgag
     1321 catctactgg aattaaaatg gaatcctgaa gttcctcttt gtgacgcaaa aaggcagcga
     1381 cgaccccgag gctggcggct tcgttggccc gagagtcatc cccgatg
//
LOCUS       DAOQDO010001987         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107205_length_1426_cov_2.605398, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001987 DAOQDO010000000
VERSION     DAOQDO010001987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107205_length_1426_cov_2.605398"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1426
                     /locus_tag="PKZ53_24370"
     CDS             <1..>1426
                     /locus_tag="PKZ53_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ43639.1"
                     /translation="IHKDIQPANILVNEATGTVRLTGFGMASRQPREHQSPNPPETIA
                     GTLAYMSPEQTGRMNRSLDSRSDLYAFGVTLYELLTGTLPFTASDPMEWIHCHIAKKP
                     LSPSHRRGDIPEPLSQIVMKLLAKAAEDRYQTAAGVEADLRTCLTSWEFAGQIDAFPL
                     AEQDISERLWIPEQLYGREREIETLVATFNRVKTTGRPELVLVSGYSGVGKTSVVTEL
                     HKALVSSRARFAGGKFDQFQRGIPYATLVQALRSLVSTILGQGESELDWWRHRISQAV
                     GLNGQLMVNLFPELELIIGPQSPAPELPPQDSQNRFQMVFRRFVSVFAQPDQPLVLFL
                     DDLHWLDMATLEFLHNIVTEDEARYLMVIGAYRDNEVTQVHPLMRMMEKLRKAGGRIQ
                     DITLAPLGTADVCRLLADAFGCDTRRVYSLATLVQEKTDGNPFFVIQFVTALAEEKLI
                     VFEPESREWTWNLEGIRAKGYADNV"
BASE COUNT          320 a          390 c          385 g          331 t
ORIGIN      
        1 gattcacaaa gatattcaac cggccaacat tttggtgaat gaagcaaccg gcacagttcg
       61 actcaccggc tttggcatgg cgtcacgcca gccgcgtgag caccagtcgc ccaatccgcc
      121 agaaaccatt gccggaacgc tggcctatat gtcgcccgaa caaaccgggc ggatgaatcg
      181 gtcgctggat tcacgcagtg acctctatgc ctttggcgtg acgctctatg aactgctcac
      241 cggcacattg cctttcacag cttcagaccc aatggaatgg atccattgtc acattgccaa
      301 aaaaccactc tcccccagtc atcggagagg cgatattcca gagccattgt cacaaattgt
      361 gatgaaactg ctggccaagg cggctgaaga tcggtaccag accgctgccg gagtcgaagc
      421 cgatctccgt acctgtctta ccagttggga attcgccggc cagattgatg catttccatt
      481 agctgaacag gatatctctg agcggttgtg gatccccgag caactctatg gccgggaacg
      541 cgaaattgaa acgctggtgg cgacgttcaa tcgagtgaag acaactggcc gccccgaact
      601 ggtgctggtt tcgggctatt cgggtgtagg caaaacttcg gttgtgaccg aattgcataa
      661 agctctggtt tcctctcggg cccggtttgc cggtggaaaa tttgaccagt ttcaacgcgg
      721 aatcccctat gccacgctgg ttcaggcgtt acgatctctg gtcagcacca ttttggggca
      781 aggagagtca gagcttgact ggtggcgaca tcgaatttct caagcggttg ggctcaacgg
      841 ccagttaatg gtcaaccttt ttcctgaatt ggaactgatc attggtccgc aatcacccgc
      901 gccagagctg ccacctcagg attcccagaa ccgctttcaa atggtcttcc gccggtttgt
      961 cagcgttttc gcgcagccgg accagccgct ggtgctgttc ctggacgatt tgcactggct
     1021 ggatatggcc acgcttgagt ttcttcacaa catcgtcacc gaggatgaag cccgatacct
     1081 gatggtgatt ggtgcctatc gggacaatga agtgactcag gttcacccac tgatgcggat
     1141 gatggagaag cttcgcaagg ctggagggcg aatccaggat attaccctgg cgccgcttgg
     1201 aaccgctgac gtctgccggc tcctggctga cgcctttggc tgtgataccc ggcgtgttta
     1261 ctcactggcc acgctggttc aggaaaaaac agatggcaat cccttttttg tcatccagtt
     1321 tgtcactgcc ctggccgaag aaaaactgat tgtctttgaa cctgagtccc gcgaatggac
     1381 ctggaacctg gaaggaattc gagccaaagg atatgccgac aacgtg
//
LOCUS       DAOQDO010001988         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107313_length_1425_cov_3.350365, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001988 DAOQDO010000000
VERSION     DAOQDO010001988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107313_length_1425_cov_3.350365"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKZ53_24375"
     CDS             <1..1047
                     /locus_tag="PKZ53_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43640.1"
                     /translation="IIILGMTSTLMVVGCLGNMPSRLVWAQTESAPAPFRTSLVKHSA
                     RWHFERALILKAQDQPAHGIVELHRAQESLKLARDIFETRFAGKELTKPQQAERDQIK
                     VELDQQELELQQLLGMLQRQNQRTVDAITALEFVIESPHPENILARLELVRAYQDLKA
                     WDKAFPHARRLVAILKVSDASSHSAIEQIRQTLPNTKLAAREATLEKAFRTLILSIEA
                     SLYADPVKFREYTLGQKVRDPLVYAELVQLFSEEDKTDEMKSMLEEAIRIQDGFCPIA
                     YLSLGDMQETAGDEAVKTNAAAAAQELYSAALKNYEIAIDQLKYLDFREEKGELDLLH
                     IQDLQKKLNQLPKH"
     gene            1086..>1425
                     /locus_tag="PKZ53_24380"
     CDS             1086..>1425
                     /locus_tag="PKZ53_24380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43641.1"
                     /translation="MATRKYKSITRIDRPDRNHHAWYVRVAYKGTLVAKHFADRKYGG
                     KRKALSEAIKFRNQTETEMGKPRTDRVVVTRNPFNKTGVIGVRRTVKRSRSVSEGRPL
                     GNVFEVTWAPA"
BASE COUNT          398 a          370 c          345 g          312 t
ORIGIN      
        1 attatcatcc tgggtatgac atcaaccctg atggttgttg gatgcctggg aaatatgcca
       61 tccaggctgg tgtgggccca aacggaatca gcgccagcgc cttttcgaac gtcgctcgtc
      121 aaacactcag cccgctggca ttttgaacgg gccttgattt tgaaagctca ggatcaaccc
      181 gctcatggga ttgtcgaact ccatcgcgcc caggaaagcc tcaagctcgc ccgcgatatt
      241 tttgaaacca ggtttgcagg taaagaactc accaaaccgc aacaggcaga gcgtgatcaa
      301 atcaaggttg aacttgacca gcaagaactt gaactccaac agttgctcgg catgcttcaa
      361 cggcaaaatc aacgcaccgt tgatgcgatt accgctctgg aatttgtgat tgaatctccg
      421 catcctgaaa atatcctggc cagattggaa ctggtccggg cctatcagga tctcaaagcc
      481 tgggacaaag cgtttccaca tgcccgtcgt ctggtggcga tcctcaaagt cagcgacgca
      541 agcagtcatt cggccattga acaaatccgc cagacactgc cgaatactaa actcgccgcc
      601 cgtgaggcca cactcgaaaa agccttccga accctgattc tctcaattga agccagtctg
      661 tatgccgacc cggtcaagtt tcgggaatac acccttggac aaaaagttcg ggacccgctt
      721 gtctatgccg aactggtaca actctttagt gaggaggaca aaaccgatga aatgaaaagt
      781 atgctcgaag aagccattcg gattcaggat ggattctgcc cgattgctta tttaagtctg
      841 ggagatatgc aggaaacggc tggggatgaa gccgtcaaaa ccaacgctgc cgctgccgcc
      901 caggaattat atagtgctgc tttaaagaac tacgaaattg ctattgacca gttaaagtat
      961 ctggattttc gtgaagaaaa aggcgaattg gacttacttc acattcagga cttgcaaaaa
     1021 aagttaaatc agctaccaaa acattgatca tgcacctacc ttaatttcat ataggagagt
     1081 tcattatggc cacccgaaag tataagtcga tcacccggat cgatcgtcca gatcgaaacc
     1141 atcatgcgtg gtatgtccgc gtagcctata aaggaacgtt agtcgccaag cactttgccg
     1201 accgcaaata cggcggcaag cgcaaagcgc tttccgaagc catcaaattc cgcaaccaga
     1261 ccgaaaccga gatggggaaa cctcggacgg atcgggttgt ggttacccgt aacccattta
     1321 acaaaaccgg cgtcattggg gtgcgtcgga cggtcaaacg gtcacgatca gtcagtgaag
     1381 ggcgcccgct gggcaatgta tttgaagtca cctgggcacc agcac
//
LOCUS       DAOQDO010001989         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107325_length_1425_cov_3.032847, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001989 DAOQDO010000000
VERSION     DAOQDO010001989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107325_length_1425_cov_3.032847"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PKZ53_24385"
     CDS             complement(<1..1047)
                     /locus_tag="PKZ53_24385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ43642.1"
                     /translation="MNHTTSQPAPPPIVAIRTELPNGLVILTEQMTSIRSVTLGIWLR
                     AGSRHEPPHLNGITHFIEHLLFKGTKNRSTRQIAIESDHLGGAVDAFTMRECTGFHIQ
                     TLDHRLAEGFDLLADLVLNPLFDPDELERERKVILEEMKMVEDTPDELAFEKFTSRFW
                     DGHGLGRPIEGTPETVETFSREIIRQYFEQTFTPANFVITAAGNLEHQQIVELANRYF
                     GSLPAGIAHLTQDLPHPTLFVDAINKPYEQAHLVLGVVAPPLLSHYRFTCQLLSTVLG
                     GGLSSRLFQRIREEEGLAYTVYSDVNAVHDAGCLNIYAAVSPANLRRTIELIMAEISS
                     LVCETVTDEELQRAR"
BASE COUNT          352 a          334 c          411 g          328 t
ORIGIN      
        1 gcgggcccgc tggagttctt cgtcggtgac tgtttcacaa accagagatg aaatctcagc
       61 catgatcagt tcaatggtcc gtcggagatt ggctggtgaa acggctgcat aaatattaag
      121 gcagcccgcg tcatggacgg cattgacgtc tgaatagacc gtgtaggcga gtccttcttc
      181 ttctcggatt cgctgaaata atcgtgagct gaggccacca ccaaggacgg ttgaaaggag
      241 ctggcaggta aaccgatagt gtgagagcag cgggggagcc accacgccca ggaccagatg
      301 ggcctgctca tagggtttat tgatggcgtc aacaaataaa gttgggtggg gaagatcttg
      361 agtgaggtga gcaatcccag ccggaagcga gccaaaatag cggtttgcca gttcgacaat
      421 ctgctggtgc tcaaggtttc cagccgccgt gatgacaaaa ttggctgggg taaatgtctg
      481 ttcaaaatac tggcgaatga tctcacgaga gaaggtttca accgtttcgg gggtaccctc
      541 aattggtcgt cccaggccat ggccatccca aaatcgggac gtaaattttt caaaggccag
      601 ttcatccggg gtatcttcaa ccatcttcat ttcttcaaga atgaccttgc gctcacgttc
      661 gagttcatcg gggtcaaaca gagggttcaa gaccaggtcg gccagcaggt caaagccttc
      721 tgccaggcga tggtccagcg tctgaatgtg aaagccagta cattcgcgca tggtgaacgc
      781 atccacggcg ccgccgagat gatcactttc aatcgcaatc tggcgggttg agcggttttt
      841 cgtgccttta aacagcaaat gctcgataaa atgggtaatc ccattcaggt gcggcggttc
      901 gtgtcgggat ccggcgcgaa gccagatgcc aagggtgacc gaccggatcg aggtcatttg
      961 ttcagtcagg atgacaaggc cattgggaag ttcagtgcgg atggcgacga tgggtggtgg
     1021 tgctggctgg gaagtggtgt gattcaagcg ttggggcatt ccttatacca ttgaggctga
     1081 agggattagg ctgagggctg agggaaatac cattttatca acagcttagc tttttgctca
     1141 tacgatagga tcattccaaa aaggtatcat atcattttgg agaaaaaaca gattgaagaa
     1201 gaatccattg actgggggaa gaaaacgggc agaaccacga ttgccctgtg cccgaaatct
     1261 tcaaccccat gtccaaagtc ttcagcccta aaaattgatt ggtgcgtctt cagtaaactg
     1321 aatcttgagg gttgagccgg gttgcagccg gatatcattg cctttggtca gcaggacccc
     1381 cgcgagtgct cctccgccaa gagttgccgc cgccgccgca ccacc
//
LOCUS       DAOQDO010001990         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107451_length_1424_cov_3.983199, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001990 DAOQDO010000000
VERSION     DAOQDO010001990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107451_length_1424_cov_3.983199"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..622)
                     /locus_tag="PKZ53_24390"
     CDS             complement(89..622)
                     /locus_tag="PKZ53_24390"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNJ43643.1"
                     /translation="MSALKQISKKWLALGFVVIFTAIVVASVMYRSPTTPKASTTSDI
                     EFLGNVTRIGFAYIKDANYDFDPKKPAPPVPVVLKNLEGKQVEITGYAMPLYNPDGKA
                     EFFLTQAANGCCFGAPPQLQHMVLVRAADPKLKLEDISHPVKVRGTFSVGEEKDSYGY
                     VTSLIRISADSIEGSRE"
     gene            complement(641..1144)
                     /locus_tag="PKZ53_24395"
     CDS             complement(641..1144)
                     /locus_tag="PKZ53_24395"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNJ43644.1"
                     /translation="MATRSRWMIFGMALTLALGFNLGLISTALPTTTVKASSAVAGAT
                     PLRIRDLAGVKVVQQAVQFPTKANELNGKLVEVNGFMVPLGLGGNKLKRFVLIEVPLS
                     CCFADGPGIDGMLYVTLAPGKELDTASDFPISVVGKFEAGVVRNQYGQVESVYRITGA
                     SARKLVQ"
BASE COUNT          350 a          384 c          316 g          374 t
ORIGIN      
        1 ttcccaggaa atcatcaatc tgaagtacct gggtttggtc tactgactac tgactactga
       61 ctactgactt atcaactact gactggtgtt attcccggct tccttcaatc gaatctgcgc
      121 taatccggat cagactggtc acgtagccgt aggaatcctt ctcttctcca actgagaaag
      181 ttccccgcac cttcaccgga tgcgaaatat cctccagctt caattttggg tcagccgccc
      241 ggaccagtac catatgttgc aattgaggtg gggcaccaaa acaacagcca ttggccgctt
      301 gtgtcaggaa aaattcggct ttcccatcag ggttgtagag cggcatggca tacccggtaa
      361 tttcgacctg cttcccttcc aaattcttca aaaccacagg cacaggcggg gcgggttttt
      421 tgggatcaaa gtcataattg gcatctttaa tgtaggcaaa tccgatcctg gtgacattgc
      481 ccagaaactc gatatcactg gtggttgaag ccttaggcgt cgtcggagac ctgtacatca
      541 cacttgctac aacaattgcg gtgaagataa cgacaaaccc cagtgccagc cactttttcg
      601 aaatttgttt cagtgcactc atctgacgta acaactcctt ttactggacc agctttcggg
      661 cactggcgcc agtaattcga tacacgcttt caacctgacc atactgattg cgaaccacac
      721 ctgcttcaaa tttcccaacc acggaaatcg ggaagtcgct ggcggtatca agttctttcc
      781 ccggtgccaa cgtcacataa agcattccat ctatacctgg accatcagca aaacaacacg
      841 aaagcggcac ttcaatcagt acaaatcgct tgagtttgtt gccgcccagc ccaagcggca
      901 ccataaagcc gttgacttcg acgagtttgc cattcagttc attggctttg gttgggaact
      961 gtaccgcctg ttgaacaact tttactccag ccagatcacg aatgcgcaac ggtgttgcgc
     1021 ccgctacggc agaagacgcc ttcacggttg tcgttggcaa agccgtcgaa atcagtccga
     1081 gattgaatcc aagcgccagg gtgagcgcca ttccgaaaat catccatcgc gagcgagttg
     1141 ccataatcac ctgctttgta gtcagtcgtc agtggtcagt agtcagtagt tcgctaagtt
     1201 tatttggttg aatcacttga ctgttttctc atacgggggt ttcagtgaaa attggtagta
     1261 tgagttataa aactgataac ctgttgttgt ccaacaggat gctatttcca gcccaattcc
     1321 ttcagcccga gcttgcgagt cttcagcccg acttcttcag ccccaagccc taaccccttg
     1381 gttttcaagc tcccaaattg gttgagacgt tggttcgata ggcc
//
LOCUS       DAOQDO010001991         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107634_length_1423_cov_2.917398, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001991 DAOQDO010000000
VERSION     DAOQDO010001991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107634_length_1423_cov_2.917398"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKZ53_24400"
     CDS             complement(<1..964)
                     /locus_tag="PKZ53_24400"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ43645.1"
                     /translation="MKADELLGLYLKGERSFAGVDLSGAQLVGAELIGVDLTDANLSR
                     ADLTGADLTGANLTRTNLTGATLNGILADNTCFQDTILPTGERWTAFVLVNEGSDGSG
                     WCRNPVDRRWPPRWLAAHSASQRRLRKLAWISPELARIVVRNPNCGSRLLKKLSHYPD
                     EQVRKGVFTHPKTPSTCILALHAEFADELAVALLKAPPDELPISTLTTLIEHQCGSLT
                     FLERVEQCLNRIPPFPAARPVLEALAKNPETPSSVLVRLARGPLARYLLRHLLRHPNI
                     DGPTLAALARFRLLESQMEIAGYPATLPTTLEQLAYVAPDVDRLI"
BASE COUNT          351 a          379 c          358 g          335 t
ORIGIN      
        1 cgatcaaccg atcaacatct ggcgcaacat aagccagttg ctccagcgtg gtggggagtg
       61 tggctggata gccagcaatt tccatctgac tttcgaggag tcgaaaccgg gccagcgcgg
      121 ccagagttgg tccatctatg tttggatgac gcagcaaatg ccgaagcagg tacctggcca
      181 gcggtccgcg agccaatcgt accaggactg acgatggagt ctcggggttt ttagcaagtg
      241 cttccaaaac tggccgggcg gctggaaaag gtggaatccg gttcaaacac tgttccactc
      301 gttccaaaaa tgtcagggaa ccacactggt gctcaatcag tgtggttaga gttgaaatcg
      361 ggagttcatc tgggggagcc tttaagagtg ccactgctag ctcgtcggca aactcagcgt
      421 gtaacgccag aatacaggtt gaaggtgttt tgggatgagt gaaaacccct ttgcgaacct
      481 gctcgtctgg ataatggctg agttttttga gcaatcgaga cccacaattt ggattccgga
      541 cgacaattcg agccagttca ggactgatcc aggccagttt ccgtaaccgt cgctgagaag
      601 cactgtgcgc ggccagccac ctcggaggcc agcgccgatc aactgggttt cggcaccaac
      661 cggatccatc gcttccttca ttaaccagca caaatgccgt ccatcgttcc ccggttggca
      721 aaatggtgtc ttgaaaacag gtattgtcag ccaggattcc gttgagcgtc gcgcccgtta
      781 aattggttcg agtcagattt gcgccagtca aatctgctcc ggttaaatca gcccggctca
      841 aattggcatc agtcaagtca acgccaatca gttcggcacc aacgagttga gcacctgaca
      901 ggtcaacacc ggcaaaggac cgttcacctt tgagatagag ccctaaaagt tcatcagctt
      961 tcataggcac gtgttgcaaa aaccagcaac catggtgagc aaattaaagc ggggggactg
     1021 ctttatacgg gtttgcccgt tctccaggca aggttctttc tgttttggaa ttttctccct
     1081 gaaccaaatc acggattgct tcgttaaggg gggcattcac aggctcaaac caatccaaaa
     1141 ttcaaatcga acgcatcgcc aaacagcact cgctgtttca aactcttttt ctttttcggc
     1201 gtctgcgttc accctgcaaa tcattccgat cacaaccgga ttcgcagaaa ccggaactga
     1261 attcatcttg aggtatcatc acacatgcgt ttgttcggcc aaaaattgtt cgttggagca
     1321 ctcgccctga gtttcctggc cggtccgttt acgaccctgg ccaatcaccc aaaagccccg
     1381 accaatccgg gttttgatcc agccaatctg gataccaaat gca
//
LOCUS       DAOQDO010001992         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107738_length_1423_cov_1.604532, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001992 DAOQDO010000000
VERSION     DAOQDO010001992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107738_length_1423_cov_1.604532"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKZ53_24405"
     CDS             <1..205
                     /locus_tag="PKZ53_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43646.1"
                     /translation="SETRIFQIQGDPRINPIKGLEQGRQPAQSNTDPAEVDTNKGLRY
                     FRREAGKFGSYPLCDDYSEESEP"
     gene            complement(350..958)
                     /locus_tag="PKZ53_24410"
     CDS             complement(350..958)
                     /locus_tag="PKZ53_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43647.1"
                     /translation="MIDSKEKNCLIARGLRLNCLTRAYADLWTEVADDSIRQEAWTSD
                     DSRLCHEFELPWADLNPGQWEWKTPLRSDFARRQALVEIDVLVALALGLTLEEVLTIY
                     RVQFPVMRQYELVDEYDARGRRLPNTTRKSQGGTEFRTAFEAWKTAGNHPHDPNAAPL
                     TVSWDIDDGRQTVTKTFYPPFSRVDREADYAQAFEKFTERFA"
     gene            1171..>1423
                     /locus_tag="PKZ53_24415"
     CDS             1171..>1423
                     /locus_tag="PKZ53_24415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43648.1"
                     /translation="MMQNGNVQLSSDFLATGEFSTQTQFGYLFHSLVCTQLAEGNFHQ
                     SESGQYRHRPPWNCILSLGFWQTVFQQKNEGRKAPNQRVI"
BASE COUNT          379 a          356 c          323 g          365 t
ORIGIN      
        1 atccgaaacc agaatattcc aaatccaggg agatccgcga atcaacccga tcaaaggtct
       61 ggaacagggc cgacaaccag cccaatcaaa tacagatccc gccgaagttg ataccaacaa
      121 agggctcaga tattttcgcc gagaagcggg caagtttggt tcctacccat tgtgtgatga
      181 ttattcggaa gaatcagaac cgtaaaggct tgaaacccgg acggactcac ccagtgttag
      241 accattttgg aataaaactc ctcgtattag cggaaggcta aaccattgaa aaaattgtat
      301 ttccttcagc tcttagcccg ataccttcag ccccaaatag tgtcagactt caggcaaaac
      361 gttcggtaaa tttctcaaat gcctgggcgt aatccgcttc ccgatcaacc cgtgaaaatg
      421 gcggataaaa ggttttggtc acggtttggc gtccgtcgtc aatgtcccag gaaaccgtga
      481 gcggtgcggc attggggtcg tgcgggtggt tcccagcagt cttccacgct tcaaaggcag
      541 tccgaaattc cgtcccgccc tgacttttgc gggtagtatt gggcagtcgc cgtcctcggg
      601 catcatattc gtccaccagt tcatattgcc gcatcactgg aaactgaacc cggtaaatcg
      661 tcagtacctc ttccagcgtc agccccagtg ccagcgccac cagtacatca atttccacca
      721 gcgcctgccg ccgggcaaaa tcgctccgga gcggtgtttt ccattcccac tggcctgggt
      781 tgaggtctgc ccagggcaat tcaaactcgt ggcacaatcg ggagtcgtcg ctcgtccagg
      841 cttcctgtcg gatggaatca tccgcaactt ccgtccaaag atcagcatag gcacgggtca
      901 agcagttgag gcgtagacca cgggcaataa gacaattttt ttcttttgaa tcaatcaatg
      961 gtactcttcc aagaatttca gaatgtaaat tacttctccc actaactctg atgacaaaat
     1021 cacagactat tgaacaagac ccagcagcaa atattagtaa tgttcttcga ttttgaaaag
     1081 ttgtacttaa tactgaattt atatgagtag tatcatacgg aattattgag ttgataaatg
     1141 ttctttcggc actaagatta agcatttgcc gtgatgcaaa acggtaatgt tcagttatct
     1201 tctgattttc tggccaccgg ggaattttca actcaaacgc agttcggtta cctttttcat
     1261 tccctggttt gtacacagct cgcggaaggt aattttcatc aatctgagtc aggtcaatat
     1321 cgtcataggc ccccttggaa ttgcatattg tccttggggt tttggcaaac ggtgttccaa
     1381 caaaaaaatg agggccggaa agcacccaat cagagggtga ttc
//
LOCUS       DAOQDO010001993         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107771_length_1422_cov_3.021214, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001993 DAOQDO010000000
VERSION     DAOQDO010001993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107771_length_1422_cov_3.021214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1422)
                     /locus_tag="PKZ53_24420"
     CDS             complement(<1..>1422)
                     /locus_tag="PKZ53_24420"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNJ43649.1"
                     /translation="LSRLIKKGYRVALCEQVEEASAAVKLVKRKVVRVITPGTAIDEQ
                     LLEGADNQYLAAVFGAGDHMAAAFLDLSTGEFQATEFAGPDHFTNTLLQLEQFNPREI
                     LCSRSLEPLFAPHIAPRSVENLPITTEDEESADQRLRLSTVLTLLDDWSFAPEQGADF
                     LTQHFEVTTLDGFGLSDKKWAIGASAAIVRYVRDTQMCDAKHITELSYFEPQEYMQLD
                     AATVRNLELVEAIQGGKRETLFGTIDLTQTGMGARLLKSWLLRPSLKLGELNARLDAV
                     GELHRQTMNRDKLRQWLTQVYDVERLLGRVSLGTANPRDLVALGSSLEPLPAMKQLLG
                     TMKSSLLQVLYESLDELTDVRSWIGEALTESPPMKIDEGNVIRVGFHAELDSLRELRS
                     NTQSFIAEVERRERERTGIASLKVKSNNVFGYFIEVTKSNLKNVPADYQRKQTIATGE
                     RFITPELKDYEEKILSAEQNILQ"
BASE COUNT          321 a          369 c          376 g          356 t
ORIGIN      
        1 gctggagaat attttgctcg gcgcttagaa ttttttcttc atagtccttg agttcaggtg
       61 tgataaagcg ctcaccggtg gcaatggttt gtttgcgctg gtaatcggct gggacattct
      121 tcaaattgga tttggtgact tcgataaaat agccaaagac gttatttgac ttgaccttga
      181 gcgaggcaat gccggtgcgt tcgcgttcgc gacgttcaac ttcggcaata aagctttggg
      241 tattgctccg aagttcccga aggctatcaa gttcggcgtg aaacccgacc cgaatgacat
      301 tgccctcgtc aattttcatg ggcgggcttt cggtcaatgc ctcgccgatc cagcttcgaa
      361 catcggtcaa ttcgtccagt gattcataca ggacctggag tagcgaactc ttcatcgttc
      421 ccagcaattg tttcatcgcc gggagcggtt ccagactcga cccaagcgcc accagatcac
      481 gcgggttggc tgtccccagt gaaacccgcc ccaacagacg ctcaacatca tagacctggg
      541 tcaaccactg ccggagttta tcccgattca tggtttgtcg gtgaagctcg ccaacggcat
      601 caagtcgggc attgagttca cccagtttga gcgatggtcg caaaagccat gatttgagca
      661 gtcgggcacc catcccggtt tgggtcaggt caatcgttcc aaagagcgtt tcacgttttc
      721 cgccctggat ggcttcgaca agttccagat tgcgaaccgt cgccgcatca agctgcatgt
      781 actcttgcgg ctcaaagtaa gaaagctccg taatgtgttt ggcatcacac atttgcgtgt
      841 cgcgcacata ccgcacaatc gcggctgagg caccaatcgc ccatttttta tcactcagac
      901 caaatccatc gagtgtggtg acttcaaaat gttgggtgag aaaatcagcg ccttgctctg
      961 gggcaaatga ccagtcatcc agcagtgtca gcacggtact gagccgaagc cgctggtcgg
     1021 ctgattcttc atcttcagtt gttattggga gattttcgac tgaacgcgga gcaatatgcg
     1081 gagcaaagag cggttctaaa cttcgtgagc agaggatttc acgtggattg aactgctcaa
     1141 gctgcaggag ggtgttggtg aaatgatctg gcccggcaaa ttcggtcgct tgaaattcgc
     1201 cggtggaaag gtcgagaaac gcagcggcca tgtggtctcc ggcgccaaaa acggcagcca
     1261 gatactgatt gtcagcccct tccagaagct gttcgtcaat ggccgtgcct ggcgtaatca
     1321 cgcgcacaac tttgcgtttt accagcttca cagcggctga ggcttcttcg acctgttcgc
     1381 acagcgcgac gcgataccct tttttgatca atcgggaaag at
//
LOCUS       DAOQDO010001994         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107782_length_1422_cov_2.773958, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001994 DAOQDO010000000
VERSION     DAOQDO010001994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107782_length_1422_cov_2.773958"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PKZ53_24425"
     CDS             <1..1217
                     /locus_tag="PKZ53_24425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ43650.1"
                     /translation="GIVLGVIRLVFVTVFAVRNRLTRSTTPEMVPDSLTLSVIIPAYN
                     EEKVICQTVESLLNATWSRFDIIVVDDGSSDATYDRVVEKFGGHPNVKAFRVANGGKA
                     AALNFGISQTRADVIVAVDADTMFHPDAIGKLARHFADPKIGAVAGNAKVGNRINLLT
                     RWQALEYITSQNLDRKAYDTLNCISVVPGAIGAWRRELIIKAGGFTHQTLAEDADLTM
                     AILRLGYRIVYEDQAIAYTEAPDSISAFLKQRFRWMFGTLQAVWKNRDAVFQPRYRAL
                     GMFVLPNVLIFQIFFPLISPLMDLMMVFAVVSTFWQRSQHPTDYSSDGLVRALAFYAL
                     FVAIDLMAAVTAFLLESREDFTLIRWLFLQRFFYRQLMYFVAIKSTLTAIRGSLVGWG
                     KLERKATVNLAQ"
BASE COUNT          300 a          340 c          384 g          398 t
ORIGIN      
        1 tgggaattgt tctgggggtt atccggctgg tatttgtgac cgtgtttgcc gttcgcaacc
       61 ggttgacccg gtcaaccacg ccagagatgg ttccagactc gctgacgctc agtgtgatta
      121 tccctgccta caacgaagaa aaggttattt gtcagacagt tgaatcactg ttgaacgcta
      181 cctggagcag atttgacatc attgtggttg atgatggttc gtctgatgcc acctatgacc
      241 gggtcgtaga gaaatttggt ggccacccaa acgtcaaagc ctttcgggtt gccaacggag
      301 gaaaagccgc ggcactcaat ttcggtatca gccagacccg cgccgatgtg attgtggcgg
      361 tggatgccga cacgatgttt catcccgatg cgattggaaa acttgcccgg cattttgccg
      421 atccaaaaat tggcgctgtg gcgggcaatg ccaaggttgg caatcggatc aatttactca
      481 cccgctggca ggcactggaa tacatcacca gccagaacct tgaccgaaaa gcctatgaca
      541 ccttgaattg tatttcggtc gttcccggtg ccatcggcgc ctggcgacgc gaattgatca
      601 ttaaagctgg tggttttacc catcaaactc tggccgaaga tgctgatttg accatggcga
      661 ttctccgcct gggctatcgg atcgtgtatg aagatcaggc cattgcctat accgaagcgc
      721 cagactcgat ttctgcgttt ctcaaacagc ggttccgctg gatgtttggc acgttgcagg
      781 ctgtttggaa aaatcgggat gcggtgttcc agccacggta tcgggcgctg gggatgtttg
      841 tgttacccaa tgtgctgatt ttccaaattt tctttcccct gatttcaccg ctgatggatt
      901 tgatgatggt atttgctgtg gtttcaacat tctggcagcg gtcacagcac ccaaccgatt
      961 attcctctga tggtctggta cgggccctgg ctttttacgc tttgtttgtc gccattgatt
     1021 tgatggcggc ggtgaccgcc tttttgctgg aatcgaggga agatttcacc ctgattcgct
     1081 ggcttttttt gcaacggttt ttctatcggc aattgatgta ttttgtcgcg attaaatcaa
     1141 cgctcactgc gattcgtggg tcactggtcg gatggggaaa actggagcgc aaggccacgg
     1201 tcaatctggc tcaatagaaa accgcctccc tggaagaata tttcagggag gcggttcttg
     1261 acggtcagtg ggttcccagg gcccgaagat tcgctctgag ctacgaaccg gcgtttcctt
     1321 cggagactga gagtcaaaac cacgtcaact cttcactggt aagtacctta tcataagttt
     1381 cctgagatat tggatttggt aagtgcttga ttttttccgt gt
//
LOCUS       DAOQDO010001995         1421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_107927_length_1421_cov_3.106881, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001995 DAOQDO010000000
VERSION     DAOQDO010001995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107927_length_1421_cov_3.106881"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKZ53_24430"
     CDS             complement(<1..387)
                     /locus_tag="PKZ53_24430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43651.1"
                     /translation="MNPIKIFRAPTLREALEQVKKELGEHALVLEHKRTRSKGFLGLG
                     SRELVELHAIANLSIRSNREESVPATEALPNLDSLNLQLDTVDLTPMTPAEAETAGST
                     TAGENYSRKSSLTSFLRAGSTTSAKDT"
     gene            complement(777..>1421)
                     /locus_tag="PKZ53_24435"
     CDS             complement(777..>1421)
                     /locus_tag="PKZ53_24435"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNJ43652.1"
                     /translation="TVVDPITVVCTHLSEVIKDFAPELLGRQETRGLLDEFAKTHPKT
                     VEETTPKVLSLGEVQRVLQNLLREHIPIRDLASILEVLADVGALTHDINALTEAVRLT
                     LSRTICSHLANEQGELAVLTLDPKVERQLSERFGLEGAAFATGLEPDFARSLLEKIET
                     TCQTAILSQPVILCSASVRPHLKKLTTRFMPQLAVISHGEVSPHIRLVALGVIS"
BASE COUNT          340 a          357 c          389 g          335 t
ORIGIN      
        1 ggtgtctttt gcggaagtcg tacttccggc gcgtaaaaac gaggtcagcg acgacttccg
       61 gctatagttt tcaccagccg tcgttgaacc tgccgtctcg gcctcagccg gtgtcatggg
      121 ggtcaggtca acggtgtcga gttgtaaatt gagtgaatcc aggtttggca gcgcttcggt
      181 ggccggaaca gactcttcgc ggttggaccg gattgagaga ttggcgatag cgtgtagctc
      241 aaccagttcg cgtgaaccaa gtccgagaaa acctttggag cgcgtgcgtt tgtgctccag
      301 aaccagcgca tgttcgccaa gttctttttt gacctgttcg agggcttcgc gcaacgttgg
      361 ggctcgaaaa attttaattg gattcataac gtttgggtga ccgtgttggg ggtgtgtggc
      421 gtgtgaccac ggttggaaat cagttccaaa cacgaaaaag atcacccatt ccaaatgcaa
      481 aacgtgtgcc ggaatttcac cgcttcacgg catcggcgtg agggttgctc agtaccttac
      541 tgaaaatttt cagacatttt caccacggaa cccacggaac ccacagaaaa caatcaacca
      601 cgcgccaaac ccaatcgctc agaaaattga tggcacggta cttataccat tttggaatga
      661 aagcctcgta ttatgagaag gctaacttat tgaaagaact gtatttccct gaaccctgaa
      721 cccgataccc tgaaccctca atggtattat caaaccagtg ctccacccct gatgccttat
      781 gaaatcaccc ccagggccac cagccgaata tgtggcgaga cctcaccgtg cgaaataacc
      841 gccagttgag gcataaaccg ggtggtcagt tttttcaaat gtggtcgaac cgaagccgaa
      901 cataaaatca cgggctgtga aagaatggcg gtttgacagg tggtttcgat tttttcaagc
      961 aatgatcggg caaaatcagg ttccagaccg gttgcaaacg cggcgccttc cagcccaaat
     1021 cgctctgaca actggcgctc gaccttcgga tcaagcgtga ggacggccag ctctccctgc
     1081 tcattggcta aatgtgaaca aatcgtgcgc gagagcgtga ggcggaccgc ttcggtcagc
     1141 gcgttgatat cgtgggtcag ggcgccgaca tcagccagaa cttcgagaat gctggccagg
     1201 tcgcggattg gaatgtgttc gcggagtaaa ttctggagga cccgttggac ttccccaagg
     1261 ctcaacactt ttggcgtggt ttcttcgacc gttttggggt gcgttttggc aaattcatcg
     1321 agcaatccac gggtttcctg gcggcccagc aattcagggg caaagtcttt gatgacttca
     1381 gaaaggtggg tgcagaccac ggtgattggg tccaccacgg t
//
LOCUS       DAOQDO010001996         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108091_length_1420_cov_3.330403, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001996 DAOQDO010000000
VERSION     DAOQDO010001996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108091_length_1420_cov_3.330403"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKZ53_24440"
     CDS             complement(<1..556)
                     /locus_tag="PKZ53_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ43653.1"
                     /translation="MFEGKPLTGDVAVVTGGGTGLGRSMALRFAELGARVVVTSRALD
                     NLESVVKEISEKGGEAHAIACDVRDYGRVEEVAAEVADRVGPATILVNNAAGNFLAPT
                     ETLSANAFNSVVGIVLNGSFHCTSVFGKQMMAAGNGGRILNIVTTYAWMGSAFVVPSA
                     CAKAGVLAMTRSLAVEWGQYKIRVN"
     gene            complement(655..>1420)
                     /locus_tag="PKZ53_24445"
     CDS             complement(655..>1420)
                     /locus_tag="PKZ53_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016950775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNJ43654.1"
                     /translation="QQVTEFNQELAAENSDIQLTIGIGINTGEVTVGYIGSKLRTDYT
                     VIGDPVNLAARLESNAKNGQILIGEQTYVAARAYMLGLHDEVLNSSLDGTAWSESGGQ
                     KFDFSMRPIGGLKVKGKTKSVQVYQVYWDGQPPESKLEPISPPPQSSPDPKNAEAGAE
                     RRKEPRIFLAIKLQIRGAGIDGRPFEEVCVTEDVSVSGACLSLVHEVRVGDQLLIRGI
                     DSVFQSQAEVRSVKFARNHYRTGIQFISNYPVWQLK"
BASE COUNT          339 a          409 c          319 g          353 t
ORIGIN      
        1 cattgactct gattttatat tgcccccatt cgacggccag cgagcgcgtc atggccagca
       61 caccggcttt ggcacaggct gagggaacca caaaggctga ccccatccag gcataggtgg
      121 tgacaatgtt gaggatccgg ccaccattcc cagccgccat catttgtttc ccaaagactg
      181 aagtacaatg aaaactgcca ttgagcacaa tgccaaccac gctgttgaag gcatttgccg
      241 acagggtttc agtcggtgcc aaaaagttgc ccgcggcatt gttgaccaga atcgtggctg
      301 gcccaacccg gtcggcgact tcggcggcga cttcttcgac gcgcccataa tcacgcacat
      361 cacaggcaat cgcgtgggct tcgccacctt tttctgaaat ttctttcacg acggattcga
      421 gattatctaa agcccggctg gtgaccacga ccctggcgcc taactcagca aatctgagtg
      481 ccattgagcg tcctaacccg gtaccgccac cggtcacaac tgcaacatca cccgtaagtg
      541 gtttaccttc aaacatgtgg ttgtgatact cccctttttt cgatggaatt gacgtttcag
      601 cattgaaaca tctttaagtt tttgcatttt tacccactaa ggcaattaaa cattttattt
      661 taattgccag accggataat tggaaataaa ttgaatgcca gtccggtaat ggttgcgggc
      721 aaatttcacc gaccgaactt cagcctggga ttgaaacaca ctatcaatcc cgcgaatcaa
      781 taactggtct cccaccctca cttcatggac cagggacagg caggcgcccg agacacttac
      841 atcttcggtc acacacacct cttcaaatgg gcggccatca atgccggccc cccggatctg
      901 aagcttaatc gccaggaaaa tgcgtggttc tttacggcgt tccgccccag cctcggcatt
      961 tttgggatcg gggctacttt gcggtggcgg ggagatcggc tccaatttcg actctggcgg
     1021 ctgtccatcc cagtaaacct ggtaaacttg cactgatttg gttttgcctt ttactttcaa
     1081 tccaccaatc ggtcgcatgg aaaaatcgaa cttctgccct ccagattcag accaggcggt
     1141 tccgtccaga ctactgttta acacctcgtc gtgtagccca agcatataag cccgagccgc
     1201 cacataggtt tgctcaccaa tcaagatctg gccgtttttg gcatttgact ctaatcgagc
     1261 cgccagattc accgggtcac caatcaccgt ataatccgtg cggagctttg acccaatata
     1321 cccaaccgtg acctcaccag tgttaatgcc aatcccgatt gtcagttgaa tatcagagtt
     1381 ttcagcagcc agttcctgat taaattcggt gacctgctgt
//
LOCUS       DAOQDO010001997         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108154_length_1420_cov_2.069597, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001997 DAOQDO010000000
VERSION     DAOQDO010001997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108154_length_1420_cov_2.069597"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..970)
                     /locus_tag="PKZ53_24450"
     CDS             complement(53..970)
                     /locus_tag="PKZ53_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ43655.1"
                     /translation="MSYQAIETHNLRKDFGTKLAVADLTLNVNRGEVFGFLGPNGAGK
                     TTSIKMLLGLVQPTAGTGSVLGAPLNNQKAKAKIGFLPEHFRFQDWLTGREFLYFHGR
                     LYGMKRQQIQARMEELLVRVDMQDAADRKLSEYSKGMLQRIGLAQALLNEPELVFLDE
                     PTSGLDPLGRLLVRDIIRDLRDQGTTVFLNSHLLSEIEVTCDRVVFVKQGRVVKEHVL
                     GEEEAEVEVELKLGTISPEIVSGLSRFGHRLVQNNGHLRLKVDSEARLPEIATWIVGK
                     GVPLYKLTSIKKPLETVFLEIMGSDERPG"
     gene            complement(1023..>1420)
                     /locus_tag="PKZ53_24455"
     CDS             complement(1023..>1420)
                     /locus_tag="PKZ53_24455"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNJ43656.1"
                     /translation="ELLVILSVMWFAGFLANNSGPVPVQVVAQSPTPQKLFVFLYSPG
                     PKWIAGKSVFEQPLENHQAYMVRLTKDKTLITGGPFADNSGAMGIIQAENLDRAWQVI
                     ELDPAVRDQVVTARVMPWTPAVSGCVGNH"
BASE COUNT          375 a          389 c          285 g          371 t
ORIGIN      
        1 cattcttcat tctcaattct tcattctcaa ttcttcattc tcaattcttc attcatcctg
       61 gtcgttcatc agaccccata atttcgagga atactgtttc aagcggcttt ttaattgagg
      121 tcaatttgta gagcggaact ccttttccga caatccaggt cgcaatttct ggaagccggg
      181 cttctgaatc cactttcagg cgcaggtgcc cattgttttg caccaggcga tgaccaaatc
      241 gagaaagacc agacacaatc tcaggtgaaa tggtacccaa tttcaattca acctcaactt
      301 cggcctcttc ttcgcccagg acgtgctctt tcacaactcg accttgtttg acaaaaacca
      361 cccgatcaca ggtcacttcg atttcactca gcaaatgcga attcaaaaaa actgtcgtcc
      421 cttgatcacg caaatcacga ataatatccc gaaccagcaa ccgcccaagt ggatcaagtc
      481 ctgaagttgg ctcatctaaa aaaacaagct cgggttcgtt cagaagcgcc tgggccaagc
      541 caatccgttg gagcattccc ttgctgtact cactcagttt tcggtcagcc gcatcctgca
      601 tatcaacccg aaccaacaat tcttccattc gcgcctggat ttgctgccgc ttcatgccat
      661 acagccgtcc atgaaaatac agaaattcac gtccggtaag ccaatcctga aagcgaaaat
      721 gctcgggtaa aaaccctatt ttggctttgg ctttctgatt gttgagtgga gcacccagca
      781 cagatccagt accagccgtc ggttgaacca gtccaagtaa cattttgatc gaagttgtct
      841 tccctgcacc attgggtcca agaaaaccga aaacctcacc ccgatttaca ttcagggtga
      901 ggtcggccac tgccagtttt gtcccaaaat ctttgcggag attgtgagtc tcaattgctt
      961 gatatgacat gaaaattgac ttgattgggt gagattttca agttcagaac tctgaacttc
     1021 aattagtgat tcccaacaca gcctgaaacc gctggtgtcc agggcatcac tctggctgta
     1081 accacctgat cccgcacggc tggatcgagt tcgatgacct gccaggcacg gtccagattc
     1141 tcagcctgaa taatccccat ggcgcctgaa ttatccgcaa atggcccgcc agtaatcagt
     1201 gttttgtcct tcgtcagcct gaccatatag gcttgatgat tctccagggg ttgttcaaac
     1261 actgacttcc cagcaatcca tttcggccct ggtgagtaca aaaaaacaaa caacttttgg
     1321 ggtgtgggtg attgtgccac aacctgaacc ggcactggtc cagaattgtt tgccagaaat
     1381 ccagcaaacc acatcaccga taagataacc agcaattcga
//
LOCUS       DAOQDO010001998         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108365_length_1418_cov_3.202494, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001998 DAOQDO010000000
VERSION     DAOQDO010001998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108365_length_1418_cov_3.202494"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PKZ53_24460"
     CDS             <1..979
                     /locus_tag="PKZ53_24460"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNJ43657.1"
                     /translation="LSFSGLGGDVKTISPLIEYKYFKSTNFLAQGKDQPAVFGMRFLA
                     QHVSGFGTPFDSNSLSFVGGIPIFSRFFLGGENTIRGYNIRSISPVAQVKNYRTTTNV
                     KAVDVLTGRTLKILKEGQTIRGIDASVLKDFTFTDDLIADPFGFPRFTQVGGDTNLLL
                     NLEYRIPIAGPVAVAMFADAGTVFNLRKLNNQNLVSNALPTTLTPFGEVLNPRGLLAT
                     QEEINDSRTPETPPGALPEGFRTILISGDQVTRSEVLLGSEFGGLRRNFRSSLGAEVR
                     VQVPVLQVPFRLIFAYNPNARVFNPSAPDPRLLAIEQKFTVRFTVGRTF"
     gene            1089..>1418
                     /locus_tag="PKZ53_24465"
     CDS             1089..>1418
                     /locus_tag="PKZ53_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43658.1"
                     /translation="MSKFRFAFYALALIVSLGFGLPQVLAQQPASQSETKIAFVNSLE
                     FSENIIEFRQQAETLDREFKPQTDQLQKLAEQLQLDEEALRRTLQDLQPDARQRRVEE
                     LEKAKKDY"
BASE COUNT          332 a          385 c          352 g          349 t
ORIGIN      
        1 cctgagcttt agcggtctgg gtggcgatgt gaagacgatt tccccgctga ttgaatacaa
       61 gtactttaaa tccacgaatt tcctggccca gggtaaagac cagccagccg tgtttgggat
      121 gcgttttctg gcccagcacg tgagtggatt cggcaccccg tttgacagca actcgctttc
      181 atttgtcggt ggaattccga ttttcagccg gttcttcctg ggcggtgaaa acacgatccg
      241 tggttataat atccggtcaa tttcaccagt ggctcaggtc aaaaattatc gcacaaccac
      301 caatgtgaaa gcggttgatg tgctgactgg ccgaacgctg aagattttga aagaaggcca
      361 gaccatccgt gggattgatg cttcggtgct caaggacttt accttcaccg atgatctgat
      421 tgccgatcca tttggatttc cacggtttac tcaggttggg ggcgatacca atctgttact
      481 caatcttgaa taccgaatcc caattgctgg cccggtcgcc gtggccatgt ttgcggacgc
      541 cggaacggtg tttaacctcc gcaagctcaa taatcagaac ctggtttcca atgccctgcc
      601 caccactctg acgccgtttg gtgaagttct taacccacgc gggttactcg caacccagga
      661 agaaatcaat gattcacgca caccagagac tccacccggt gcattgccag aaggattccg
      721 gacgattttg atctccggtg atcaggtgac gcgtagcgaa gtcttattgg gcagtgaatt
      781 tggtgggtta cgacgaaact tccgatcctc tttaggggct gaagtgcggg ttcaggtgcc
      841 ggtgctccag gtgccgttcc ggctgatctt tgcatacaac cccaacgctc gggtcttcaa
      901 cccaagtgcg ccagatccac gattgctggc cattgagcaa aaattcaccg tccggttcac
      961 cgtcggacgg accttctaaa aattcaacgg attgagaggt tggggttgga ttcagtgacc
     1021 acgccgacac aggttcacgt cccaccccct tcccccatcc gctaactcct aaccctggaa
     1081 gaagtagtat gtcgaaattc cgttttgctt tttatgcact tgccctgatc gtcagccttg
     1141 gttttggtct tccgcaggtc ctggcccaac aacctgcctc ccagagtgaa actaaaatcg
     1201 cgtttgtgaa ttcactggag tttagtgaaa atattatcga gttccggcag caggctgaaa
     1261 ctcttgaccg cgagtttaag ccgcaaactg accagcttca gaaactggct gaacagctcc
     1321 aactcgacga agaagccctc cgccgcacct tgcaagacct ccagccagat gcccgccagc
     1381 gtcgggttga agaacttgaa aaagccaaaa aagactat
//
LOCUS       DAOQDO010001999         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108509_length_1417_cov_2.878855, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010001999 DAOQDO010000000
VERSION     DAOQDO010001999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108509_length_1417_cov_2.878855"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PKZ53_24470"
     CDS             <1..1099
                     /locus_tag="PKZ53_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43659.1"
                     /translation="RFLNIADPWSDPALKLWSGYSKTFFEDSAGRISAVSAPDSKPVD
                     MIPLALYGLDFPKIPALLVDFRRVLNPKLREVSRQAINDISRFVLDITPFRNLSGYLV
                     QKCAAIIPNKKGFDINQPSRAVTYAQLKSLLMLKDLFDPELKAMIVRDLETANVNPLA
                     DERNLEQSFAKAQYQALMTDVERNQLDRQIEQDRRRERVQLKYGTGKTALFKMLQLAS
                     VGIFRKKADTPDDIQTYLTARSMRYHLKLLEQVAQTPAPIEVGWDPSRFLPSLEFVAR
                     NSNQAPASVVGVLEKIYRNSTDTGVQLASVEGLAAFKRPEAKLALARIERDSRLIQLS
                     RARSVRGLRKEPSTPVMAEPSGWLSLMPAQN"
     gene            1148..>1417
                     /locus_tag="PKZ53_24475"
     CDS             1148..>1417
                     /locus_tag="PKZ53_24475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43660.1"
                     /translation="MPKKPASYLELVLGCALIFCSGWTATVRATSQREPRQPLRLVIM
                     KVDGLPPPVVERYISQINPRTGRSVLPWIEKLFFSNGIRVENFYTT"
BASE COUNT          350 a          389 c          351 g          327 t
ORIGIN      
        1 acgatttttg aacattgcag atccctggag tgacccggcg ttgaaattat ggtcagggta
       61 ttcaaaaacc tttttcgagg actcagccgg tcggataagt gccgtatcag caccggactc
      121 aaagccggtg gacatgattc cactggcact ctatggcctt gattttccaa agattccggc
      181 tttgctggtt gattttcgcc gggtactcaa ccccaaactg cgcgaagttt cccgtcaggc
      241 catcaatgac atcagccggt ttgtgctcga catcacgccc tttcgcaact tgagcggata
      301 tctggttcaa aaatgtgccg ccatcattcc aaataaaaaa gggtttgata tcaaccagcc
      361 ctcccgagcg gtcacctatg cccagcttaa atccctcttg atgttaaaag atctctttga
      421 tccagaactc aaggccatga tcgtccgcga tttggaaacg gcaaatgtga acccgctggc
      481 tgatgaacgg aacctggagc aatcttttgc caaagcgcaa tatcaggcac tgatgaccga
      541 tgttgaacgc aaccagcttg accgccagat tgagcaggat cgccgtcgcg agcgagttca
      601 attgaaatat ggaaccggga aaaccgccct ttttaaaatg ctccagcttg cttccgtcgg
      661 gatctttcga aaaaaagctg acactccaga cgatattcaa acctacctga ccgcccggtc
      721 aatgcgctat cacctcaaac tgctggagca ggttgcccag acaccagcgc ccattgaagt
      781 tggttgggat ccaagccgct ttctgccctc gcttgaattt gtggctcgca acagtaatca
      841 ggcgccagcc agtgtagtgg gggtgctcga aaaaatttac cggaattcga ccgataccgg
      901 ggttcaactg gcttcagttg aaggactcgc ggcattcaag cgcccagaag ctaagctggc
      961 gctggcccga atcgaacggg atagccgatt gatccagctt tcgcgagccc ggtccgtgcg
     1021 aggactacgc aaagagcctt caactccggt tatggccgaa cccagcggct ggctgtcgct
     1081 catgccggcc caaaattgat accagggaac cccaacgtag tgacacgtca ccgcattcca
     1141 aattgttatg ccgaagaaac cagctagtta tcttgaattg gttttgggtt gcgccctgat
     1201 cttttgttca gggtggacag caaccgtcag ggcgaccagt caacgtgaac ctcgccagcc
     1261 gctccgactg gtcattatga aagttgatgg gttgccacca cctgtggtcg aacgatatat
     1321 cagccagatc aacccgcgaa ccggacgcag tgtgctgccc tggattgaaa aactgttctt
     1381 ttcgaacggc atccgggtcg aaaattttta caccacc
//
LOCUS       DAOQDO010002000         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108513_length_1417_cov_2.752570, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002000 DAOQDO010000000
VERSION     DAOQDO010002000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108513_length_1417_cov_2.752570"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PKZ53_24480"
     CDS             complement(<1..1402)
                     /locus_tag="PKZ53_24480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43661.1"
                     /translation="MPRIFDIRPANSPFHLKIEYISGSPLSDVMAANCGNLDFCLRVG
                     REMLDALHHLAEHKILHRDISPSNILIPDTDNAPIRLIDFGLASATEATQTAVGTPLY
                     RAPEIDRGGYWRVQCDLYSLGVVLFELLTGRLPYVLTERGVPRKDQESVYDTSQVSEE
                     FRRVVKVLRKVTSFDPNQRYGSAAEVSEALRLARIPKYQSDTGGTQRENPFVKDLRSV
                     YRNSRLGNAENRGLDSQFARKTYVQTQLDRELLPQILSRTLKLVILSGNPGDGKTTFL
                     AKVAEEVNRQKGQPISRDDSGWVMQIGGHQFAALYDASESHKDQSADALMHQLLLPLA
                     GETEPRVHYTALIAANDGRLLDFFERFGKEHYPWLWSHLQQQLFDGKPADSGVVLVDL
                     KSRSLTGWQQEETSLFSQILDEFVRPDRWAICQECVSRRECPMYFNVQSLQDPVLKPG
                     IVRQLHRMLLAVHLKRE"
BASE COUNT          354 a          384 c          343 g          336 t
ORIGIN      
        1 gctcgcgttt caaatggacc gccagcaaca ttcgatgaag ttgacgcacg ataccaggtt
       61 tcaggactgg atcctgcagg ctctggacat tgaaatacat tgggcattca cgtcgtgaaa
      121 cacattcctg gcagattgcc catcggtcag gccggacaaa ttcatccaga atctgagaaa
      181 acaacgatgt ttcttcctgc tgccacccgg tcagtgatcg ggatttcaaa tccaccagca
      241 ccacaccaga atcagccggc tttccatcaa acaactgttg ctgcaggtgt gaccagagcc
      301 atggatagtg ttctttccca aaccgctcaa aaaagtccag cagccgcccg tcattagcgg
      361 caatcaaggc tgtgtaatga accctgggtt cggtctctcc ggccagtggc aacaacaact
      421 ggtgcatgag cgcatcagcg gactgatctt tatgggattc tgaggcgtca tagagcgcgg
      481 caaactggtg cccgccaatt tgcattaccc atcccgaatc atctcgcgag atgggttggc
      541 ccttttgtcg gtttacttct tcggcaactt ttgccagaaa cgtggttttt ccatcgccgg
      601 gattgccact caaaatgacc agcttcaacg tccggctcaa aatttgaggt aacaactccc
      661 ggtcaagttg agtctgaaca taggttttgc gagcaaactg cgaatccagc cctcgatttt
      721 cagcattgcc cagccggcta ttgcgataga cgctgcgcaa atctttaaca aaaggatttt
      781 cccgctgcgt gccgcctgta tcagattgat attttggaat ccgcgccagt cgaagtgctt
      841 ctgaaacctc tgctgccgag ccatatcgtt ggttcggatc aaaagaagtg actttccgta
      901 acactttaac cacacgtcga aactcttctg acacctgact ggtgtcatag accgattcct
      961 gatctttgcg cgggacaccg cgttcggtca ggacataggg aagcctgccg gtcaacaatt
     1021 caaacagcac caccccgagt gaatataaat cacactgcac cctccagtac ccgccccggt
     1081 caatctcagg ggcccggtat agtggcgtcc ccacagcggt ttgggtggct tcggtggcgc
     1141 tggccagccc aaaatcaatc agccgaatcg gggcattgtc agtatccgga attaaaatat
     1201 ttgacggcga aatatcgcgg tgcaggattt tgtgttccgc cagatggtgc agggcatcaa
     1261 gcatttcccg gcctacccgg aggcaaaaat caagattccc acaattcgcc gccatgacat
     1321 cactcaatgg agaccccgaa atatattcga tttttaagtg aaacgggctg ttggcggggc
     1381 gaatatcaaa aattcgtggc aaatttggat ggtttaa
//
LOCUS       DAOQDO010002001         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108519_length_1417_cov_2.728341, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002001 DAOQDO010000000
VERSION     DAOQDO010002001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108519_length_1417_cov_2.728341"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKZ53_24485"
     CDS             <1..315
                     /locus_tag="PKZ53_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43662.1"
                     /translation="DCYLNTGDWKSAEPLAVHFPKYIGLFERNLAALTVLAARNKELD
                     EALRLWARKDNLNRLELTALPDLIRLGMGNHLLRYYQQMKLADPESVIPDKVLPLLQS
                     NE"
     gene            complement(450..1316)
                     /locus_tag="PKZ53_24490"
     CDS             complement(450..1316)
                     /locus_tag="PKZ53_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNJ43663.1"
                     /translation="MSYWNYYPKASKPREAKGGIKAKSQRGDIGETWWSKRFIKLLES
                     FKLGARLTRGKTYARKGQVLNLGIEPGEVTAKVQGSARTPYTISIRIQPFTERQWQDV
                     EIALAQQALFMAKLLNNEMPNEIEQAFNACGLNLFPASVREIKSNCSCPDSSNPCKHI
                     AAVYYILAEKFDENPFEIFFWRGRSQEKLITNLRNLRGADDEPDPTPEPVLESVEPVI
                     PLSQSLHNFWDMGESAARLQFKPQAAKVPDAILKQLDPLPLEISGVPVTDILSELYEV
                     FTSQAEKKAFSE"
BASE COUNT          318 a          330 c          359 g          410 t
ORIGIN      
        1 gattgttatc tcaacactgg tgactggaaa tcggctgaac cccttgccgt tcactttcca
       61 aagtacatag gcttatttga acggaacctg gcggcgctta cggttctcgc tgccaggaat
      121 aaggaactgg acgaggcctt gcggttatgg gcccgcaaag acaatctgaa ccgtcttgag
      181 ttgacagcgc ttccagatct gatccgcctg ggtatgggga accacctgct tcgctattac
      241 cagcagatga aactggcaga ccctgaatca gtcattccgg ataaagtgct gcctttgtta
      301 cagtccaacg aatagctttt cagataaata tttcctgagt ttgtatttta ggaagtctca
      361 gaacccattg aagaatttgg atttattttc tgtgtgtttc tgtgtattcc gtggtttctt
      421 tatctgaaat tctatatttt gctacgttgc tactcactaa aggctttctt ctctgcctga
      481 ctggtaaaga cttcatatag ctctgataat atgtcggtta caggcacacc actgatttca
      541 agcgggagcg ggtcgagttg tttcaaaatg gcatccggaa ctttggctgc ctggggtttg
      601 aactggagtc tggccgctga ttcgcccatg tcccaaaagt tgtggagtga ctgggataac
      661 ggaataaccg gctcgactga ttcaagaact ggttctggtg ttgggtcagg ttcatcatct
      721 gcaccccgga gattgcgcaa gtttgtaatg agtttttcct gcgaacgtcc gcgccagaag
      781 aagatttcaa acggattttc gtcaaatttc tcagccagaa tgtaatacac ggcggcaata
      841 tgcttgcacg ggtttgaaga gtcagggcag gaacaattcg acttaatttc ccggaccgaa
      901 gccgggaaga ggttcaatcc gcaagcgtta aatgcctgtt cgatctcgtt tggcatttca
      961 ttgttcagta atttggccat aaacagcgcc tgctgggcga gggcaatttc aacgtcctgc
     1021 cactggcgct cggtgaaggg ttgaatccgg atcgaaattg tatagggagt ccgtgctgac
     1081 ccctgaactt tggcggtgac ttcaccgggt tcaatcccaa gattcaatac ctgtcctttc
     1141 cgggcatagg tttttccacg ggtcagtctg gcgccaagct taaatgactc cagcaacttg
     1201 ataaagcgtt tcgaccacca ggtttcacca atatcacccc gttggctttt ggctttgata
     1261 ccgcctttgg cttcacgggg tttagaggct ttcggataat aattccagta actcataccg
     1321 gttgccctcc tttggttttc gagggtttgg aaacggcttt tttcgaaccg ggtttggctt
     1381 tggccgaagc ttgggttgag gcttcggcct cggccac
//
LOCUS       DAOQDO010002002         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108658_length_1416_cov_3.083027, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002002 DAOQDO010000000
VERSION     DAOQDO010002002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108658_length_1416_cov_3.083027"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1220
                     /locus_tag="PKZ53_24495"
     CDS             192..1220
                     /locus_tag="PKZ53_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ43664.1"
                     /translation="MKIIDLHTHILPANWPNLKDKYGYGGWPRLEYLSPCHAKIMIDD
                     RFFREITDNCWDPEVRLKEGGVDIQVLSTVPVMFSYWARPQDAYDLSRFLNDHLAELV
                     NRWPDRFVGLGTLPLQAPDLAIQELKRCVQELGLAGVEIGSHVNDWNLDQPELFPVFQ
                     AAAKLGASVFVHPWDMVGRERMPQYFLPWLVGMPAESSLAICSVIFGGVLERLPQLKI
                     CFAHGGGAFPYTLGRINKGYEARPDLCQIHITHPPRTYLPQIYVDSLVHDPDALLYIL
                     KLFGANRVAMGSDYPFPLGEFPPGNLIKSMAELDELTRERLLWGTAAEFLGLEKRMVP
                     WEALENEE"
BASE COUNT          345 a          322 c          361 g          388 t
ORIGIN      
        1 tttcgctggt caaccaaatc ggtcgccaaa gtgatggcca aatgtgaacc ggccaccagt
       61 tcagccaagg cggcatgatt tcatacccca tttatgggga cttgtactaa gcatcaggta
      121 ctgaccactg accactcgcc actaaaaggt ttcttcattc tcaattcttc attctcaatt
      181 cttcattctt tatgaaaatc attgatttac acacccacat ccttccggca aattggccca
      241 atctgaagga caaatatgga tatggcggct ggccacggct ggagtacctg tcaccttgcc
      301 acgctaaaat catgattgat gatcggtttt ttcgtgaaat caccgataat tgttgggatc
      361 ctgaggtgag gttgaaagaa gggggcgttg atatccaggt tttgtcgacg gtgccggtga
      421 tgtttagtta ttgggccagg ccgcaggatg cctatgattt atcccggttt ttgaatgacc
      481 accttgctga actggtcaac cggtggccag atcgatttgt gggacttggc accttacctc
      541 ttcaagcgcc agatctggcc attcaggaac tcaaacgctg tgttcaggag cttggtctgg
      601 ctggggttga aatcggaagc cacgtcaatg actggaacct ggatcaacca gaactctttc
      661 cggtgtttca agccgcagcg aagctggggg cgtcggtgtt tgtccatccc tgggacatgg
      721 ttggccgtga gcggatgccc cagtattttc tgccctggct ggttgggatg ccggctgagt
      781 cttcgctggc gatttgttca gtgatttttg gaggagtttt agagcggctt ccacaattga
      841 aaatttgctt tgcccatggt ggcggtgctt tcccctatac gcttggtcgg attaacaaag
      901 ggtatgaagc tcgacctgat ttgtgccaga ttcacattac ccatccaccc cggacctatt
      961 tgccccaaat ctatgttgat tcactggttc atgatccgga tgcgttgctc tatattctga
     1021 aactctttgg agctaatcga gttgcaatgg ggtcggatta tccatttccg cttggggaat
     1081 tcccccctgg aaacctgata aagtccatgg ccgaactgga tgaactgacc agggaacggt
     1141 tgttatgggg aacggcggca gaatttctgg ggcttgaaaa gaggatggta ccgtgggaag
     1201 cgcttgagaa tgaagaatga agaattgaga atgaagaatt gagaatggag aatgaagaaa
     1261 cacctggtga ctggttctta gtactcggta cttggttctt ggaaggtatt gatccctaag
     1321 caccaggtac tcactaagta ctttaccgaa aatttccaga cattttaacc acggaaacca
     1381 ctgaaaaaaa tcaactactt accaaaccca gtatct
//
LOCUS       DAOQDO010002003         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108739_length_1416_cov_2.057311, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002003 DAOQDO010000000
VERSION     DAOQDO010002003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108739_length_1416_cov_2.057311"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PKZ53_24500"
     CDS             <1..1107
                     /locus_tag="PKZ53_24500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43665.1"
                     /translation="SVPTNDGFEYFCGNIYTTTWRVTDPYSTPPDQDSYPALLRGAGI
                     AQTMLGVTAAAGTAPAASQTGAVNWRIVDSAKTGRTRAFRRGVRIVNGWDIPRWYLKK
                     DGPVPRHFTGMSLVCENPVYIYGNLNAIGVQEQPSTIIDPDSGLPVDGPTASRRFLQT
                     GNRNMPLVPGDFNSATPVSGGADDGLLHGPMSIMADAVSIQSRAYQDARMFISQGGHS
                     YRPQNRLRRYAMETTVKAAWLTGQPKAGVQTTSQQTIEGTGDSVDLNSGNSPNTQGGV
                     HNFPRFCETWTWPTGQVRFNYNGSFIYQFYSQQGNGPWKIGGWNNASHYNAPPRNWNF
                     DSAFKKLGGLPPGTPRVSFYKIRGFRQVFIEDQD"
BASE COUNT          346 a          367 c          375 g          328 t
ORIGIN      
        1 tcggtgccga ccaacgatgg atttgaatat ttctgcggga atatctatac cacaacctgg
       61 cgggttaccg atccatactc aacgcctcca gaccaggaca gttatcctgc gctgcttcgc
      121 ggggccggga ttgcacaaac catgttgggt gtaaccgctg ccgctggaac tgcccctgcc
      181 gccagccaaa ccggagccgt caactggcgg atagttgatt cggccaaaac gggtcgtacc
      241 agagccttcc gacgaggggt gcggattgtc aatggatggg atatcccgcg ctggtacctt
      301 aagaaggatg gtccggttcc acgtcacttt accggcatgt cgctcgtttg cgaaaacccg
      361 gtgtatattt acggtaacct gaacgccatt ggggttcagg aacaaccatc aacgattatt
      421 gatcctgact ctggcctccc agttgacggg ccgacggcct cacggcggtt ccttcaaact
      481 ggaaaccgaa atatgccgct ggtaccaggt gactttaact cagccacgcc agtatcgggc
      541 ggcgctgatg acggcttact ccacgggcca atgtcaatta tggcggatgc ggtctcgatc
      601 caatcccgtg cctaccagga tgcccgaatg tttattagtc agggcggaca ctcataccgt
      661 ccacaaaacc gattgcgccg ctatgcaatg gagacaactg tgaaggctgc ctggttgacc
      721 gggcaaccaa aagcgggtgt tcaaactaca tcgcaacaaa cgattgaagg tactggtgat
      781 tcagtcgacc tgaattccgg aaactcaccc aacacccagg gcggggtgca caacttccca
      841 cgtttctgcg aaacctggac ctggccaacc gggcaggttc ggtttaacta taatggatcg
      901 ttcatctacc agttctatag ccagcagggg aacggaccgt ggaagattgg tggttggaac
      961 aatgcctcac attacaatgc cccacctcgg aactggaact ttgactcggc attcaaaaaa
     1021 cttggcgggt tgccaccagg aacgccgcga gtgagtttct ataagatccg aggcttccgt
     1081 caggtcttta tcgaagacca ggattaagca cagggagtag ggaacagggg acaggggata
     1141 gagatcaatt caagaaaatt gagctttgaa ctgtttgacc ctaacttatc cacaagcgtt
     1201 gaaggcaggt gcgaaggcac ctgccttctt tgttttctta gagacatatt tctacttcta
     1261 ccggattttg tgtttcgcca ggtcggcgtt gaaataaagg gctggtcagt accacctgcg
     1321 tgagcctaga ttttgtgcaa acagctcaga aagatgattt ttggtcagag ttcaagcttt
     1381 cgctggtgaa aaccttgttt cggcctcatt caaagt
//
LOCUS       DAOQDO010002004         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_108959_length_1414_cov_4.091979, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002004 DAOQDO010000000
VERSION     DAOQDO010002004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108959_length_1414_cov_4.091979"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1133)
                     /locus_tag="PKZ53_24505"
     CDS             complement(192..1133)
                     /locus_tag="PKZ53_24505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNJ43666.1"
                     /translation="MKYLTRTQKLDIRQIGLHRALFPCVVFLRGDKLGLAMRLEKTMT
                     VIGRGSDVAIQLDGDDEASRSHAQIRSIEDPSGQRQFWLMDLGSTNGTILNGVLLERH
                     KEILLNDGDKFSIGRHILKFAMLDDLDEEYHRRIHELIMHDDLTGLLTRKSFCIELDR
                     ELARSMRHNHEFCVLMLDIDFFKKVNDSYGHLVGSQVLAEVSLVIRQTLRDSDVAGRY
                     GGEEFIALLPETNKIRGLEAAERIRQLIERQPFTGSVHDSSVKSHITISIGVASYPAD
                     GSTPTMLIDNADIALYHAKQTGRNRICGYSSNLVHTP"
BASE COUNT          382 a          328 c          341 g          363 t
ORIGIN      
        1 gctattgact gatgaagggt cgaacgccag tcaaagggta aagtttcttt ttctgattgt
       61 cgagacaatg atggcaggta attcgatttt gaggagggaa caagccagta gcaaggaaaa
      121 atggggaacc actcaccgtg ggcattcgcg acccgggccg catccatcaa ccagcgatcc
      181 accgggttgg gctatggggt atgcaccaga tttgatgaat atccacaaat ccggtttcgg
      241 ccagtttgct tggcgtgata gagggcgatg tcagcattgt caatcagcat ggttggggtt
      301 gatccatctg ccggatagct ggccacacca atactgatgg tgatgtgtga tttcacactt
      361 gaatcgtgta cagagcctgt aaatggctgt cgttcaatga gttgccgaat tctttcagcc
      421 gcttcgaggc cacggatttt gttcgtttcc ggcaggagtg caataaactc ctcacctcca
      481 tatcggccag ccacatccga atcccgcaac gtctgacgaa tcaccaggct gacttcggcc
      541 aggacctgac tccccaccag atgcccgtag gaatcgttga ccttcttaaa gaaatcaata
      601 tcgagcatca acacacaaaa ttcatgattg tggcgcattg agcgggctaa ctcacggtcg
      661 agttcaatac aaaaactttt gcgcgtcagt aatccggtca gatcatcgtg catgatcagt
      721 tcatgaatcc ggcggtgata ttcctcatct aaatcatcga gcatggcaaa tttgagaata
      781 tgccgcccaa tcgaaaactt gtcgccgtca ttgagtaata tttctttgtg tcgttcgagc
      841 aataccccgt tcaaaatcgt gccattggtg ctcccaaggt ccatcagcca gaactgacgt
      901 tgcccggatg ggtcttcaat gctccgaatt tgggcgtgtg accgtgaagc ttcatcatcc
      961 ccatcaagct gaattgcaac atctgacccg cgcccaatca cggtcatcgt tttttccagc
     1021 cgcatcgcca gtcccaattt atctccgcgc aaaaatacaa cacagggaaa gagtgcacga
     1081 tgcaacccaa tctggcgaat atcgagtttt tgagtacgtg tcagatattt cacggtgatc
     1141 tcaaaaaatc cctttcacaa gtttggcaat atccctggaa ataaattgaa tacaagaagc
     1201 tgaggaaatt caacgtggac agagagttca gtttggaaac tggaaataaa accagtgcgc
     1261 atcagcataa attcggaatg agcaagttgc aagtgacaca ccgaagaatg aagaattgag
     1321 aatgaagaat tgagaattga gaattgagaa tgaagaaccg tttggttctt ggttcttggt
     1381 tcttggttct tggttcttgg ttcttggttc ttgg
//
LOCUS       DAOQDO010002005         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109010_length_1414_cov_2.573216, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002005 DAOQDO010000000
VERSION     DAOQDO010002005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109010_length_1414_cov_2.573216"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1414
                     /locus_tag="PKZ53_24510"
     CDS             77..>1414
                     /locus_tag="PKZ53_24510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43667.1"
                     /translation="MPEWANLPPGVHLITGMEQVLGLCHDEKWVYVGHPWGVSIFDFQ
                     GNLVTRVATQSAAASFAPLNGDLWIGTPTGLLKLDCQTFTLAQLPVDWGMTADRLQEI
                     YTNQKNRRDELTVNENQVDPLNGVQSLVPQGDQLWISTQHSICRYNPRTQELRVYPTD
                     EITNLSLGNWDALWVDGQYVWARSSLRGYPPHFRFFRYDPHTDTWASYQDQNQNLTRI
                     LGNINGKLWVYFTYPDQLEGFGYLKSQSLEIIPVSKPIPSWLVYCGKAEGKMIFQDSR
                     SSKFFSLDEESNTLKAVGESAQKIETDIPSALWYSTLRVGITGTISGQIHSNRHEVFG
                     QFLRSGGVHLLRLPNGTQIIGAAGGYTFNYFELSEWSLIPSLRFPLTPTTCGLRFRFP
                     DGRVRLTTLPENQKNILGNIIYFLTFDPATSSIWLGTDYGVSVLNENLELLRTF"
BASE COUNT          364 a          337 c          334 g          379 t
ORIGIN      
        1 tcttcaaaat tcccgatctc gtgaaacttc aactgatctg acaccaggga ttggactttc
       61 tggaccagga aaaaaattgc cggaatgggc aaacctgccg cctggggtac accttatcac
      121 cgggatggag caggttcttg gtttatgtca cgacgaaaag tgggtgtatg tcggtcaccc
      181 atggggggtt tcgatctttg attttcaggg aaatctggtc acacgcgtgg ccacccaaag
      241 tgccgccgcc agtttcgccc ctctcaatgg tgatttgtgg attgggacac caactggtct
      301 tttgaaactg gactgtcaaa ccttcacact cgctcagttg cctgtcgatt ggggtatgac
      361 ggctgacagg cttcaagaaa tttacacaaa tcaaaaaaat cggcgggatg agctgacagt
      421 caacgaaaat caggtggatc cccttaatgg agttcaaagc cttgttcccc agggcgacca
      481 gttatggatc agtacccagc atagtatttg ccgatacaat ccccgcacac aggaacttcg
      541 ggtttaccca acggatgaaa taaccaatct cagtcttggc aattgggatg cactgtgggt
      601 tgatggtcag tatgtctggg ccagatcttc gttgagaggc tatccaccac acttcagatt
      661 tttccgatac gatccacata ccgatacctg ggccagttat caagaccaga accagaatct
      721 cactcgaatc ctcggaaata taaatggaaa attatgggtg tactttacct acccagatca
      781 acttgaaggg tttggctatc tcaagagcca gtcacttgaa attatcccag tttcaaagcc
      841 tatcccgtca tggcttgttt attgtggaaa ggctgagggg aaaatgattt tccaggattc
      901 ccgctcctcc aaattctttt cgcttgatga ggaatcaaac acattgaagg cagttgggga
      961 atcagcacag aagattgaga ctgacattcc gagcgcactt tggtattcga ctctcagagt
     1021 tggaatcact ggaacgattt ctggacaaat tcattccaat agacatgagg tctttggaca
     1081 gtttcttcga tctggcggcg ttcacctgct tcgcctcccc aacgggactc agatcattgg
     1141 ggctgctgga ggatatacct tcaattattt tgaattgagc gaatggtcat tgattccatc
     1201 acttagattt ccgctcacac cgacaacctg cggtctgcgc ttccgatttc ctgatggacg
     1261 ggtccgttta acgactctac ccgaaaacca aaagaatatt ctggggaata tcatttattt
     1321 cctgaccttc gatccggcaa ccagctcgat ctggctgggg actgattatg gggtgagtgt
     1381 cctcaacgaa aatttggaat tgcttcgaac tttt
//
LOCUS       DAOQDO010002006         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109169_length_1413_cov_2.606038, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002006 DAOQDO010000000
VERSION     DAOQDO010002006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109169_length_1413_cov_2.606038"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PKZ53_24515"
     CDS             <1..792
                     /locus_tag="PKZ53_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43668.1"
                     /translation="LKPQLSSSTYQGLSQTTNQADAIEFAVTARVNQPSTTGAGARGG
                     QTLYAPPPVTRLPYTQTAAVNDSKSNKLTLGIVIASIVVLGIGGVALVVKKNTKTETT
                     EVVKTPVEANSGLTGNSSAYRESFDRLSTNIHQAVDLVPVSGPSSPGELKLARDQVDH
                     AQAVVGTSANDLGMTEPPTAEIRVPHENLKDRYQALKTSLDNYSIALRNYEFALTSTG
                     EYNARDRDNPPGTAERAEIYRCQSAFRDAWREAQTYEQRLRTQLK"
     gene            complement(872..>1413)
                     /locus_tag="PKZ53_24520"
     CDS             complement(872..>1413)
                     /locus_tag="PKZ53_24520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43669.1"
                     /translation="CKKDRGRIATNEYLEVPGWPGVWAVGDAASIPDPSGKPYPPTAQ
                     HAIRQGKVLADNIIASLRGGKKKPFLFKTLGLLSALGKRSGVANILGFQFSGFVAWFL
                     WRSIYLSKLPGFDKKIRVMIDWTLDLIFSKDLVQFMTLRAPSVSHAENGNGHHHQHQH
                     PAPAPPVQVSTPHSTMSHV"
BASE COUNT          370 a          364 c          353 g          326 t
ORIGIN      
        1 ttaaagccac agctttcgag ttcaacgtac cagggattga gtcaaaccac caatcaggcg
       61 gatgcgattg aatttgcagt cacggccaga gtcaaccagc cttcaaccac tggggctggt
      121 gccagaggtg ggcagactct atatgccccc ccgccagtga cgcgtcttcc ctatacccaa
      181 acggcagcgg tgaatgactc aaaatcaaat aagctaaccc ttggaattgt gattgcttca
      241 attgttgtgc ttggaattgg gggcgtagcg ctagtcgtca agaaaaatac aaaaacagaa
      301 accactgaag tggtgaaaac gccggttgag gccaattcag gactgaccgg aaatagttct
      361 gcctatcgcg agagttttga ccggctctca accaatattc atcaggctgt ggatttggta
      421 cccgtttcag gtccttcatc accaggagaa cttaaactgg ctcgcgacca ggttgatcat
      481 gcccaggcag tagttgggac ttcagccaat gatttgggaa tgactgagcc cccaaccgca
      541 gagatccggg tgcctcacga aaacctcaaa gaccgctacc aggcattaaa aaccagtttg
      601 gacaattaca gtattgcact ccgaaactat gagtttgcgt tgacttcaac tggcgagtac
      661 aacgccaggg accgagacaa tccccctgga accgctgagc gggctgagat ttatcgctgc
      721 cagtcagcct ttcgcgacgc ctggagggaa gctcaaacct atgaacaacg gttacgcacc
      781 cagctcaagt agccagttcc aaaagcaaaa cccgcagcgg tgagctgcgg gttttatatt
      841 tcgagagttg tgctgtgggt ccaatatcct tttatacgtg tgacatcgtt gagtgcggtg
      901 ttgaaacctg taccggaggt gctggtgctg ggtgctggtg ctggtgatga tgcccattcc
      961 cgttctcagc atgtgataca gacggtgccc gcaaggtcat aaactgaacc agatcctttg
     1021 aaaaaatcag atccagcgtc cagtcaatca tcactctgat ctttttatca aagccaggaa
     1081 gcttgctcaa ataaattgac cgccacaaaa accaggccac aaaccctgaa aactgaaacc
     1141 cgagaatatt ggccactcca gatcgctttc caagcgccga aagcagtccc agggtcttaa
     1201 agaggaacgg ttttttcttg ccacctcgaa gtgaagcaat gatgttgtca gccaggactt
     1261 ttccctggcg gatggcgtgt tgggccgttg gtggataagg tttcccagat ggatctggaa
     1321 ttgaagcggc atctcccact gcccaaaccc ctggccagcc agggacttcc agatattcat
     1381 tggttgcaat tcgtccccga tcttttttac agg
//
LOCUS       DAOQDO010002007         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109290_length_1412_cov_3.593957, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002007 DAOQDO010000000
VERSION     DAOQDO010002007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109290_length_1412_cov_3.593957"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PKZ53_24525"
     CDS             complement(<1..160)
                     /locus_tag="PKZ53_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNJ43670.1"
                     /translation="MSEPKDPRPTDAELQLLKILWERGPSTVREVHETLNQTKPTMYT
                     TVLKTFQIM"
     gene            complement(197..1180)
                     /locus_tag="PKZ53_24530"
     CDS             complement(197..1180)
                     /locus_tag="PKZ53_24530"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ43671.1"
                     /translation="MKFKLILLVCFSIVFLLPAKAANPHKPVEKRQRLVAVTFDDLPS
                     PEKLDVQLLETLTRNLLKTFTQYKIPVVGFVNEGKLEVENEKAARTRVLKLWTDAGFE
                     LGNHTYSHPYFFKKPLVEFEENVIKGEPVTRQLVAEKGMKLRYFRHPFLSTGPDAATK
                     AAFEKFLTERGYTVAPVTIDNSDWLFAGVYADAQKRGDTEMMRRVAEAYVPYLERVFE
                     FHEKLSREVVGYEVRQILLLHANSLNADHFGKVVEMMKRRGYQFISLEEALQDKAYRL
                     PDTYIGANGISWLQRWAITKGGNYREEPALPTFVTEKWAWIAEEKLKQEKR"
BASE COUNT          337 a          328 c          350 g          397 t
ORIGIN      
        1 ccataatctg gaacgttttc aggactgtgg tgtacattgt tggtttggtt tggtttaagg
       61 tttcgtggac ttcgcggacg gtgctcggac cgcgttccca caaaattttc agcaattgga
      121 gctccgcatc ggtcgggcgt gggtctttgg gttcactcat atttaggttc aggggttaga
      181 gttcaagatt caggggttat cgtttttcct gcttcagctt ttcttcagcg atccaggccc
      241 atttctcggt gacaaatgtg gggagtgcgg gttcttcacg atagttccca cctttggtga
      301 tagcccagcg ttggagccac gaaataccgt tggcgccaat gtaggtatca gggaggcgat
      361 aggctttgtc ctgcagcgct tcctccaggg aaatgaactg atatccccgt cgtttcatca
      421 tttcgaccac tttgccaaaa tggtcggcat tgagcgaatt ggcgtgcagg agcaaaatct
      481 gacgcacttc atacccgaca acctcgcggg acagtttctc gtggaactcg aaaacccgtt
      541 ccagataggg cacatacgct tctgccactc gccgcatcat ttcggtatcg ccgcgtttct
      601 gggcatcggc atagacaccg gcaaacagcc agtcggaatt gtcaatcgtg actggtgcca
      661 ccgtgtatcc acgttcggtc aggaattttt caaaagcagc cttcgtcgct gcatcgggtc
      721 cggtacttaa aaaaggatgg cggaagtaac gcaatttcat tcccttttca gccactaact
      781 gcctcgtgac gggttcacct ttaatgacat tctcctcaaa ttcaaccagt ggttttttga
      841 aaaagtatgg atgcgaatag gtgtggttgc cgagttcaaa cccagcgtcc gtccagagtt
      901 tcaaaactcg ggtgcgagcg gctttttcgt tctcgacttc cagcttacct tcattgacaa
      961 atccaaccac cgggattttg tactgagtga atgttttgag caagttgcgg gtgagagttt
     1021 cgagtaattg aacatcaagt ttttctggcg atggcaggtc atcaaacgtt acggcgacca
     1081 gtcgctgccg tttttcaacg ggcttgtgtg ggttggcagc cttcgcaggg agtaaaaaca
     1141 cgatggaaaa acacacaaga agaatcagtt tgaatttcat gagattttga gtttgagcgt
     1201 gtagactggg tgacagcagg tatcaagaaa tatactaatt atttcgtacg agatatatag
     1261 tacgaaaaac ttcgtagatg tcaattgctt ttttcgggtt cagggaaccg acaaaaaaga
     1321 cataacttca aaaaccctga accctaaacc ctgaaccctg aaccctgtat ttcattgatc
     1381 ttttttccga tttcgacgtt ttcgactccg ac
//
LOCUS       DAOQDO010002008         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109303_length_1412_cov_2.991157, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002008 DAOQDO010000000
VERSION     DAOQDO010002008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109303_length_1412_cov_2.991157"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1412
                     /locus_tag="PKZ53_24535"
     CDS             <1..>1412
                     /locus_tag="PKZ53_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNJ43672.1"
                     /translation="KVALLLFGSGMCALIYQTAWLREFRLIFGASTAASAAVLGIFMG
                     GLGLGGLLIGKRVDQNAQPLRLYGHLELLIALSTALTPFLVWVIRQLYIVFGGSTTLG
                     LTGGTLVRLLFSALVLSVPTFLMGGTLPAAARAVETEGDQNRRNLALLYGMNTLGAVT
                     GTVLSTFYMLEIYGNRATLWTACLVNMAIALAALKLAPGMIVENVDSETEPVQSEAGD
                     DLASGSATRQGVPAMFILAAAGLVGFAFLLMEIVWYRMLGPLLGGSTFTFGLILAVAL
                     LGIGLGGAIYSVFGGNRTPTISGFALTCVLEAVCIAIPYALGDRLALLTILLRPLGSI
                     GFSGHLLGWSIITVITIFPAALVAGIQFPLLIALLGKGKKDIGQQVGLAYAWNTVGAI
                     IGSLAGGFGLLPLLSAIGTWKLIVFLLVGQGGIALALSFRESGNVIRLIPSSMAAVLA
                     VLLILTAGPTTAWRHSPIGAG"
BASE COUNT          267 a          314 c          407 g          424 t
ORIGIN      
        1 aaggttgcac tcttactatt tggatcaggc atgtgcgcac tgatatatca gactgcctgg
       61 cttcgtgaat ttagacttat ttttggggct tcaacggctg catctgctgc ggtgcttggg
      121 atttttatgg gaggcctggg attgggtggc ttactcattg gcaaacgagt ggatcaaaac
      181 gctcaaccct taaggttata tggtcacctt gagttattga ttgcactgag cacggcgctg
      241 accccttttc tggtatgggt tattcgccag ctttatattg tttttggtgg ctcaaccacg
      301 cttggcctga cgggaggaac cctggtcagg ctcctttttt cagctctggt actcagcgtg
      361 ccgacatttt taatgggagg gacattgccg gctgctgcac gtgctgttga aacggaggga
      421 gatcagaatc gtcgaaacct ggcgttactc tatgggatga atacgctggg tgctgttacg
      481 gggacagtgc tctcaacctt ttatatgctt gagatttatg gaaatcgagc cacattgtgg
      541 acagcttgtc tggtcaatat ggccattgcc ttagctgcgc tcaaacttgc cccaggaatg
      601 atcgttgaaa atgtagattc tgaaaccgag ccagttcaat ctgaagcggg tgatgatttg
      661 gccagcggat ctgccactcg tcaaggggtg ccggcgatgt ttattttagc ggcagcggga
      721 ctggttggat ttgcctttct gctcatggaa attgtctggt accgcatgct gggtccgtta
      781 ttgggtgggt caaccttcac gtttggttta attttggcgg tggctttact gggaatcgga
      841 cttggaggtg ccatctattc agtttttggg ggaaatcgca caccaactat ttcaggtttt
      901 gccctgacct gtgtgctcga agcggtatgc attgccattc catatgcgct gggagatcgg
      961 ctggcactct taaccattct cttgcgcccg cttgggtcaa ttgggtttag tggtcatttg
     1021 ttgggatgga gcatcatcac tgtcattact atttttccag ccgcgctggt tgccggtatc
     1081 cagtttccgt tattgattgc cctgcttggg aaaggaaaga aagatattgg acaacaggtc
     1141 ggtctggctt atgcctggaa caccgtgggc gccattattg gatcactggc cggaggcttt
     1201 ggcctgttgc cgctcttgtc ggcgattggg acctggaaat tgatcgtttt tttactggtc
     1261 ggtcaagggg gaattgcgct ggccttgagc tttcgagaat ctgggaatgt gatccggcta
     1321 atcccatcca gtatggcggc tgttctggcg gtgctcctga tcctgactgc tggtccaacc
     1381 acagcctggc ggcatagccc cattggtgct gg
//
LOCUS       DAOQDO010002009         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109329_length_1412_cov_2.563007, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002009 DAOQDO010000000
VERSION     DAOQDO010002009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109329_length_1412_cov_2.563007"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKZ53_24540"
     CDS             <1..927
                     /locus_tag="PKZ53_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43673.1"
                     /translation="ANALFGSNGVKSLINEFDYPRLGPGQMWGRFQQAVEDRGGAVHL
                     NTTIVRLNYEGNRVTSVVARRDGQEFEIAAPHFISSMPINKLVLHMNPQPPEAVVKAA
                     QSLSYRAFVLVGLIVRRENLFPDNWIYIHSPELSVGRIQNFGNWSAAMVPEPGKSCLG
                     LEYFCNAGDDLWKTPDEELIRLAGKELMQLGLAREGDVEDGVVIRQPKAYPVYDSEYR
                     GHLEVIKSFLDQFTNLQTVGRNGMHRYNNQDHSMLTGMLAVKNILGEKHDLWDVNTER
                     SYYEEFTTTDAPKPASAHLHSAGVIPQFHEAE"
     gene            complement(1034..>1412)
                     /locus_tag="PKZ53_24545"
     CDS             complement(1034..>1412)
                     /locus_tag="PKZ53_24545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43674.1"
                     /translation="KPDEALVYFLKGVKVKPDWVEGYFHLGEIYTLLARQQHSAQYLA
                     SAITQYQTAIERRPDFAAARLSLANLLLGIEKIAEAEAEVQTVLNQSPDSPGAHLLLS
                     KILRKQGKLSEAEAHERRAKGAK"
BASE COUNT          327 a          346 c          387 g          352 t
ORIGIN      
        1 gccaacgcgc tgtttggttc aaacggggtg aagtcgctca ttaatgagtt tgactatcca
       61 cggctgggac cggggcagat gtgggggcga ttccagcagg cagtggaaga tcgcggcggc
      121 gccgtccatc tcaacacaac gattgtgcgc ctcaattatg aagggaaccg ggtcacaagt
      181 gtggttgcac ggcgagatgg tcaggaattt gaaattgccg cgccgcactt tatttccagc
      241 atgccaatca acaagctggt gctccatatg aacccccaac cgccagaagc ggtggtaaag
      301 gcagcccaaa gtttgagtta tcgcgccttt gtcctggtcg ggctgattgt caggcgcgaa
      361 aacctgtttc cagacaactg gatttatatt catagcccgg aactgagtgt tggtcggatt
      421 caaaattttg gcaactggag cgccgccatg gttcccgaac cagggaagag ctgcctgggc
      481 ctcgaatatt tttgcaatgc cggcgatgat ttgtggaaaa ctccggatga agaattgatt
      541 cgcctggctg gcaaagaatt gatgcaactg gggctggccc gtgaaggcga tgttgaggat
      601 ggggttgtaa ttcgtcagcc gaaagcctat ccggtgtatg acagcgaata tcgcggccac
      661 ctggaagtca tcaaatcatt tctcgaccag tttaccaacc tgcaaacagt tggccgcaat
      721 ggcatgcatc ggtacaacaa ccaggatcac tcgatgttga ccggaatgct ggccgtaaaa
      781 aacattctgg gtgagaagca cgatctgtgg gacgtcaaca ccgaacgatc ctactatgaa
      841 gaatttacca ccacagatgc tcccaagccg gccagtgccc atcttcacag tgccggcgtg
      901 attcctcaat ttcacgaggc cgaataggta atgccagttt gtcgtcagta gatcgtcgtc
      961 agtcgctcgc taactctatt tgattgaatt ctttaaccgt tttctgatgc gagaacttca
     1021 tggcaaattg gtattatttc gctccttttg ctcttctttc atgcgcctcc gcttcgctga
     1081 gtttgccttg ttttcgcagg attttgctca gcaacaggtg ggcaccaggg gaatccgggc
     1141 tttggttgag cacggtttgg acttcggctt cagcttccgc aattttttca atgccaagca
     1201 gaaggttggc cagactcagc cgggcagcgg caaagtcagg gcgccgttca atcgcggtct
     1261 ggtactgagt gatggctgag gcgagatatt gcgctgaatg ctgttgcctg gccaggagcg
     1321 tatagatctc accgagatga aagtatccct cgacccagtc tggtttgact ttgacccctt
     1381 tgagaaaata gaccagtgct tcatcaggtt ta
//
LOCUS       DAOQDO010002010         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109488_length_1411_cov_2.470501, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002010 DAOQDO010000000
VERSION     DAOQDO010002010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109488_length_1411_cov_2.470501"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..>1411)
                     /locus_tag="PKZ53_24550"
     CDS             complement(366..>1411)
                     /locus_tag="PKZ53_24550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43675.1"
                     /translation="MALEIRRAADRGAELVRELMAFGRRTEANRAIIDVNEVVLETTS
                     LMQRLIGPHIQVDLDLDETCPHICADASQLTQVLLNLAANARDAMPTGGILTIQTRLV
                     SVDEQTLSELIDRPGKTVVSTNPITVGKKVKVTVRDTGAGIPPEVLKRIFEPFFTTKD
                     IGKGTGLGLATVYGFVNGTGGFIEVYSRPEYGTTFHIYLPSANQETLIGIDYDPLLPN
                     LEEGTRTVLVVDDEPAVRKMVKELLHQNGFHLLEAGSGEEALALERSFKGTIDLILSD
                     LLMPKMNGYELVARFLTRRPAARALMMTGDGSRITAETEHTLLPLLAKPFTEEQLLAA
                     VRRSLTGRQKTEG"
BASE COUNT          352 a          356 c          355 g          348 t
ORIGIN      
        1 ctctccaggc aaaattcgat aaacaaggat tgagattgac agcctggaaa acctttaagg
       61 aaaggaaata gatgggcgac agttgcaagc ccccgatgta caaggtatcg attttcaaaa
      121 aaatatttcc tgggagtacg ggtatctcgc cgcactaaat ctattgataa caaatgagtt
      181 gtgagcgaga tactcgcgcc cctaaaccga aatcttttcc tgaagaacta taacgaaaaa
      241 attcatgtcc aaacaaagaa ttgctgattt cacccgaagg cgcgccgggc ctatctggaa
      301 acaagatggt gttgccattg gaaacagact catcagcaat tcacattttg ggttattggc
      361 atccttcatc cttcagtctt ctgtctgccc gtcagcgagc gccgaaccgc tgccagaagt
      421 tgttcttccg taaagggttt ggccagtaat ggcagcaatg tgtgttcggt ctcggcagtg
      481 attctggagc catcaccggt catcataagg gctcgggcag caggtcgccg ggtcaaaaag
      541 cgggcaacca gttcgtatcc attcattttc ggcatcagta aatcactcaa aatcaaatca
      601 atcgtgccct taaaggaccg ttccagggcc agggcttctt cacctgaacc ggcttccagc
      661 aggtgaaaac cgttttgatg gagcaattct ttcaccattt tgcggacagc gggttcatca
      721 tcaaccacca gcaccgttcg ggtgccttct tcaagattgg ggagcaatgg gtcataatca
      781 atcccgatga gggtttcttg attggcactg ggcaggtaaa tatgaaacgt ggttccatat
      841 tcgggtcgtg aatacacctc aataaagcca cctgtcccat tgacaaatcc ataaacggtg
      901 gccagtccta acccggtacc cttgccaatg tccttggtgg taaagaatgg ttcaaaaatt
      961 cgcttgagca cttcgggcgg aatgccggcc ccggtatctc gaaccgttac ttttaccttc
     1021 tttccaaccg tgattggatt ggtgctgaca accgtttttc cagggcgatc aatcagttca
     1081 ctcagcgttt gttcgtcaac cgaaaccagc cgggtttgaa tggttaagat gcctccggtg
     1141 ggcattgcat cgcgggcatt ggccgcgagg ttgagcagta cctgggtcag ttgactggca
     1201 tcggcacaaa tatgggggca ggtttcgtcc aggtccagat caacctggat gtgcggtccg
     1261 atcagacgct gcatgaggct ggttgtttct aaaaccactt cgttgacgtc aataatggcc
     1321 cgattggctt cggtgcgacg gccaaaggcc atcaattccc gcaccagttc ggcaccccgg
     1381 tcggcagccc ggcgaatttc gagggccatc t
//
LOCUS       DAOQDO010002011         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109630_length_1410_cov_2.657565, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002011 DAOQDO010000000
VERSION     DAOQDO010002011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109630_length_1410_cov_2.657565"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKZ53_24555"
     CDS             complement(<1..1106)
                     /locus_tag="PKZ53_24555"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNJ43676.1"
                     /translation="MLRRITLLAALLLVAVVSWTQTPRRWTSGDIHEGIRKLNFLGSA
                     LYVGAHPDDENTRLIAYLANEVKANVAYLSLTRGDGGQNLIGPEIAELLGVIRTQELL
                     AARRVDGGKQFFSRANDFGFSKHPDETFRIWNRDEVLSDVIWAIRRWQPDVIINRFDH
                     RTPGRTHGHHTASAILSVEAFDMAGDPKVHPEQLALAGPWQPKRLFFNTSWWFYGSRE
                     KFDQADKTNLMSVDVGVYYPIKGKSNTEIAAESRSMHRCQGFGSIGERGSESEYLEIL
                     KGEMPTDKSDIFAGINTTWSRVPGGAPIGEILAGVERNFKYENPSASVPELMRAYKMI
                     KALPDGYWKRVKLGEITEVIKACLGLFAEAIASD"
     gene            1219..>1410
                     /locus_tag="PKZ53_24560"
     CDS             1219..>1410
                     /locus_tag="PKZ53_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ43677.1"
                     /translation="MSHKTKLGELASTAICGNDISSSVLYVSALAIGFAGQYAWITLL
                     IVAVILFLFRRIYGEVVGAL"
BASE COUNT          335 a          361 c          372 g          342 t
ORIGIN      
        1 taatcactgg caatggcttc agcaaaaagg ccgagacagg ctttgatgac ttccgtaatc
       61 tctcccaatt tcacccgctt ccaatagccg tcggggagtg ctttgatcat tttgtaagca
      121 cgcatcagct caggaacact agccgacggg ttttcatatt tgaaattgcg ctccacgcct
      181 gccaaaattt cgccgatggg cgctccaccg ggcacacgcg accaggtggt attgatccca
      241 gcaaagatgt cggacttatc tgtgggcatc tcgcctttga ggatttcaag gtattcggat
      301 tctgaaccgc gttcgccgat ggaaccgaat ccctgacagc ggtgcatact ccggctttcg
      361 gcagcaattt ctgtattgga ttttcctttg atgggataat aaacacccac atccacactc
      421 atcaggttgg ttttgtcggc ctggtcaaat ttttcgcggc tgccatagaa ccaccaggaa
      481 gtgttaaaaa acaaacgctt gggttgccag gggccggcca aggccaattg ctcagggtgc
      541 actttcgggt caccggccat atcaaaggct tccacagaga ggatggcaga tgccgtgtgg
      601 tggccatggg tgcgcccggg ggtgcgatga tcaaaacggt tgatgatcac atcgggctgc
      661 caccggcgga tggcccagat gacatctgac aaaacttcgt cgcggttcca gatccggaat
      721 gtttcgtcag ggtgttttga gaaaccaaaa tcattggcac ggctgaagaa ctgcttgccg
      781 ccatcgaccc tgcgcgcagc gagcaattcc tgggtgcgga tgacccccag cagctcagca
      841 atctcagggc ctatcaggtt ctggccacca tcgccacggg tcagggataa gtaggccaca
      901 ttggctttga cttcattggc caggtaagca atcaaccggg tgttttcatc atcggggtgt
      961 gcacccacat aaagggcgga acccagaaaa ttgagtttcc ggatgccttc gtggatatcg
     1021 ccggaggtcc agcgacgagg tgtttgggtc caggagacca cggctaccaa taaaagggcg
     1081 gccaaaaggg taattcttct aagcatgatc gcgttgcttt ggtataaaaa tcatttcaat
     1141 ctaaccattc gcaggggtta tcagtttaaa gtttgccaaa attaatagct ttgagggatt
     1201 gaccaatcac tcagccccat gagtcacaaa acaaagcttg gagaactggc ttctaccgca
     1261 atctgtggga atgacatcag ctcatccgtt ttgtatgttt ctgcgctggc catcggattt
     1321 gccgggcagt atgcctggat caccctgctc atagtagccg ttatcctgtt cctattccga
     1381 agaatttacg gcgaagtagt aggcgccctg
//
LOCUS       DAOQDO010002012         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109653_length_1410_cov_2.323247, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002012 DAOQDO010000000
VERSION     DAOQDO010002012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109653_length_1410_cov_2.323247"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKZ53_24565"
     CDS             complement(<1..1024)
                     /locus_tag="PKZ53_24565"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43678.1"
                     /translation="MKCLPFQVTLSYTLVLIVITGLGSIGRLPNSQTVKPATDQERAQ
                     LEIEKLDAEAKNLYRAGKYDQGIVLARQAVEQSEKIFGPEHTLLATRLNLLANFHRAK
                     AEFQKAETLYQRALAIQEKSFGPAHPDVATSLHNLGLVYQATGDFQKAESHYQQALAI
                     QEKSLGTEHLDVSITLNNLGNLYRNTGDYQRAEPLYQRALAIKKKVLGVDHPSVATSL
                     TGLANLYLEKGNYKKAEPLYQEGLAIREKALGANHLDVANSLTNLALLYNEKGDYQRA
                     QSLYHRALAIYEKSLGPDHPKLTACLNNLGKLSWDQGNYQQAEQYLQRALAIREKPLG
                     PDHPDTA"
BASE COUNT          302 a          343 c          385 g          380 t
ORIGIN      
        1 acgcggtatc cgggtggtca ggccccaggg gtttttcccg gatggccagc gcccgctgga
       61 ggtattgttc cgcttgctgg tagttgccct gatcccacga gagtttgccc aggttgttga
      121 ggcacgcggt gagctttgga tggtcagggc ccagcgattt ctcatagatg gccagtgccc
      181 gatggtacaa agattgggcc cgctgatagt cccctttttc attgtagaga agtgctaaat
      241 tggtgagact gttggccaca tccaggtgat tggctcccag cgctttctcc cggatagcca
      301 gcccttcctg gtacagcggc tcggcttttt tgtagttgcc tttttccagg tagagattgg
      361 ccaaccccgt gaggctggtg gcgacacttg ggtggtcaac gccgagtact ttcttcttga
      421 tggccagtgc ccgctgatag agtggttcgg ctcgctggta gtcacctgtg tttcggtaga
      481 ggttccctaa attgttgagg gtgatagaca catccaggtg ttcagtgccc agtgatttct
      541 cctggatagc cagcgcctgc tgatagtgtg attcggcttt ttgaaagtca cctgtcgcct
      601 gataaaccag ccccagatta tggaggctgg ttgcaacatc tggatgagct ggaccaaaac
      661 ttttctcctg aatggccagg gctcgctgat agagtgtttc agccttctga aattcggctt
      721 tggcacgatg gaaattggcc agcaggttga gcctggttgc cagcagcgta tgctctgggc
      781 caaagatttt ttcgctctgt tccaccgctt gccgggccag gacgatacct tgatcatatt
      841 ttccagcgcg atacaggttt ttcgcctcag catcgagttt ctcaatttca agctgagccc
      901 gctcctggtc cgtcgctggt ttgactgtct gggagttggg aaggcgaccg atggaaccca
      961 gacctgtgat gacgatcagg accaaggtat aagacagcgt cacctgaaac ggcaagcatt
     1021 tcatggggta aattcctttc aaattcaaat caaaactgag gttaagcaaa aggatgacga
     1081 ctggtcgaag gcaacttgga atgtcaaggt ttatggttgc caagtttatc acccaacgtt
     1141 tgcccgcgta acaccagatt ttgaattgat tgtgctcaca aagccgttca tcggcgaaca
     1201 atcgccgtcc caatttcgca cgtccctcag accaatcatt ccagaattca gaatgtaact
     1261 cattgaaaat acagtggaat ctcaaacaac gactctggca catcaattga gttgcacaaa
     1321 cggatccggt ttcaatcttt ggcctaagga gagacttgct tatggattcg ctggttcggg
     1381 atgtgcgttt tggggtgcga atgctcttga
//
LOCUS       DAOQDO010002013         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109699_length_1410_cov_1.946125, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002013 DAOQDO010000000
VERSION     DAOQDO010002013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109699_length_1410_cov_1.946125"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1410
                     /locus_tag="PKZ53_24570"
     CDS             25..>1410
                     /locus_tag="PKZ53_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43679.1"
                     /translation="MDNILIVTGGNGTKMAEATVRLLATGFPTQSANGTVTSIGDTLQ
                     IWRVDPDRSSGALSSLQRCLDEYKKLQHLMNDGNGSPRPTHLATSPWAMEVNTHIKDF
                     DPFKLTKQSASIKNLRELLGKAPGKNEAKPILNAFYENKDLEVEIDRGFYQKPFIGAP
                     VMAAFAESLDNQNSPAGELINLNSLKNREVRFFLCGSIYGGTGASALPVLGQFLAKQK
                     LDHDLNHWKIGGCVLMPYCLPPAPPFNALPEHEQNDPVRIREESFRLAQIHASHPAFI
                     NLTIEERHKLARQILQGFYADPKGLHLRARHSLVYYSHILKATFDELYLVGKPEPDEL
                     KVWSNGGQSQTNPLNSAEVVAAISALNFFSGTEVCRREAYGLTTGTAEMQSQALRLSD
                     LPCYKVNEQVVDPEKVVLATSLLIHLLKHQIQWGHDAQGWDSDPGGLRKVYEMSPDRQ
                     RADRVGYETALN"
BASE COUNT          377 a          360 c          359 g          314 t
ORIGIN      
        1 cccacaatca taaaaggagc aagtatggat aacattttga ttgtaacggg cggtaacggc
       61 accaaaatgg ctgaagcaac cgttcggcta ctggcaaccg ggtttccaac tcaaagtgcc
      121 aatggcaccg tgacgagtat cggagacacc ttgcaaatct ggcgggttga cccggaccgc
      181 agctccggag ccttaagttc tcttcagcga tgccttgatg agtataagaa attgcaacat
      241 ttgatgaacg atggcaacgg gtcaccgaga ccgactcacc tggcgaccag cccctgggcc
      301 atggaggtca acactcatat taaagatttt gacccgttta aactgacaaa acagtcggcg
      361 agtatcaaaa acctgagaga actcctgggg aaggcgcctg gaaaaaacga agcgaaacca
      421 atcctgaatg ccttttatga aaacaaagat ttagaggttg aaattgatcg tgggttttac
      481 caaaaaccat ttattggcgc tccagtcatg gcggcgtttg ctgaatctct cgataatcaa
      541 aactcaccgg ctggggaact gatcaatttg aattccctca aaaatcgtga agttcggttc
      601 tttctgtgcg gcagcatcta tggcggtact ggcgccagtg ccttgccagt cctcgggcaa
      661 tttcttgcca aacaaaaact cgaccatgac ctcaatcatt ggaaaatcgg cggatgcgtg
      721 ttgatgccct attgcctgcc accagcgccc ccgtttaatg ccttgcccga acacgaacaa
      781 aacgatccag tacgaattcg ggaagaatcc ttccgcctgg cccagatcca tgcgagtcac
      841 cctgctttta tcaatttaac cattgaagag cgacacaaac tggcccggca aattcttcag
      901 ggtttttacg cggatccgaa agggttgcac ctgcgtgccc ggcatagcct ggtctactac
      961 agccatattt taaaagcgac gtttgatgaa ttgtacctgg ttggaaaacc agaaccggat
     1021 gaactgaaag tgtggagcaa tggcggccaa agtcagacca atccgctcaa ctctgccgag
     1081 gtcgttgccg ccatcagcgc actcaacttc ttttccggga ctgaagtgtg ccggcgtgag
     1141 gcatatgggt tgacgaccgg cacggctgaa atgcaatcac aggcacttcg gctttcagat
     1201 ctgccctgtt acaaagtgaa tgaacaggtg gtggatccgg aaaaagtcgt gctggccaca
     1261 tccttgctca tccatttact caaacatcag attcaatggg ggcacgatgc tcaagggtgg
     1321 gatagcgatc cgggcgggct ccgcaaggtg tatgagatga gccctgaccg ccagcgagct
     1381 gatcgagtgg gctatgagac tgccctgaac
//
LOCUS       DAOQDO010002014         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_109782_length_1409_cov_2.649188, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002014 DAOQDO010000000
VERSION     DAOQDO010002014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109782_length_1409_cov_2.649188"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..737)
                     /locus_tag="PKZ53_24575"
     CDS             complement(171..737)
                     /locus_tag="PKZ53_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HNJ43680.1"
                     /translation="MAEIFVSTDIEADGPIPGPYSMLSFASAAYRADKTLISTFSANL
                     ETLPGASGHPLTMTWWETQRDAWIACRQNMRPPEEAMKEYLVWLKQLPGKPVFVAYPA
                     AFDFMFVQWYLIRFTGESPFAHSALDIKSYAMAMLKSNFRETVKPALPRHWFDDLPHT
                     HVALDDALEQGALFCNMLADNLKLKDGQ"
     gene            complement(995..1408)
                     /locus_tag="PKZ53_24580"
     CDS             complement(995..1408)
                     /locus_tag="PKZ53_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ43681.1"
                     /translation="MFPFHLAFPVTNLEATRSFYADLLGCQVGREAERWIDFDLFGHQ
                     ISAHLADSVVGKVASNDVDSKQVPIPHFGVILDWDTFHRLADRLQTARIEFIIEPYIR
                     FQGEVGEQATMFFQDPSGNYLEFKAFKDMASIFAR"
BASE COUNT          383 a          324 c          370 g          332 t
ORIGIN      
        1 gtcgtcagtc gtcagtcgtc agtcgtcgag tgttggggtt ttgaatggtc tgtcacgtct
       61 atttggttct ttttaagtca gttatgaaca atttttttca ggaactctat gaagtcaacg
      121 atctgggttt tatctactga ctgatggacg actgacgact gactgatgga ctactgaccg
      181 tctttcaact tcaaattatc ggccagcata ttgcagaaga gcgctccctg ttcaagcgcg
      241 tcgtcaaggg cgacgtgcgt gtgagggagg tcatcaaacc agtgccgtgg gagggctggt
      301 ttgacggttt cacggaaatt ggatttgagc atggccatgg cgtagctttt aatatccaat
      361 gctgagtggg cgaaggggct ttcacccgta aaccgaatca aataccactg cacgaacata
      421 aaatcaaagg cggctggata ggccacaaac accggtttac cgggaagttg tttgagccag
      481 acgagatact ccttcattgc ttcttccgga ggccgcatgt tttgacgaca ggcaatccag
      541 gcgtcgcgct gggtttccca ccaggtcatc gtcaatggat gaccggacgc accgggtaag
      601 gtttccagat tggctgaaaa cgtgctgatc agggtcttat cagctcgata ggcggctgaa
      661 gcaaagctca gcatggaata tggacctgga attggtccat ctgcctcaat atcagtactc
      721 acaaaaattt ctgccatgtt caaacgaata cctttctttg atcaaaaaag cggcgaaagc
      781 tggcacttta ccgcacattc ggctggttcg aacaggggag acgcacaact tgttcacaaa
      841 aggggagtct gaatctggct ttatcgaggg caaaagcgtc gtcaaattgc aaatcgaact
      901 cgaactggtc ggaaaaaacg aaactgaaag cgaagaataa aaaagcgtac cggaagcgtc
      961 actgggtgac gcccccggca aaatttcaac cgggttagcg tgcaaaaatc gatgccatgt
     1021 ccttaaaggc tttaaattca agataattgc cgcttggatc ctggaaaaac atagttgcct
     1081 gttcgccgac ttcgccttgg aaccggatat agggttcgat gatgaattca atccgggcgg
     1141 tttgcagtcg gtcagccaga cgatgaaacg tgtcccagtc cagaatgacg ccaaagtggg
     1201 gaatcggcac ctgtttggaa tcaacatcat tggacgcgac ttttccaacg acggaatccg
     1261 ccaggtgcgc cgaaatctga tggccaaaca ggtcaaaatc aatccagcgc tcggcttcac
     1321 gcccaacttg gcatccgagc aagtcggcat aaaacgaacg cgtggcttca agattggtta
     1381 caggaaaggc aagatgaaat ggaaacata
//
LOCUS       DAOQDO010002015         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110021_length_1408_cov_2.032520, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002015 DAOQDO010000000
VERSION     DAOQDO010002015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110021_length_1408_cov_2.032520"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1408
                     /locus_tag="PKZ53_24585"
     CDS             <1..>1408
                     /locus_tag="PKZ53_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43682.1"
                     /translation="MSPLTTLNAYVKFAAVKTDSGVTIVAATSEFDTVKGCGSGALAG
                     RGGVLRRSTDSGVTWSEPVSGISGYCGGQCFYDIAVAMSPLDPKVIYIGGQGRVGQVG
                     NCASITFARSGDGATFARRERGLYVDTHAIEVSTTNPSIVYTGNDAGIFKSTDEGMNW
                     TSLNTPDLATILFHSVALHPTNRNLCIGGTQDLGTEFRRADGTWSQTDISDGGAVAID
                     RNVRDTSNVLMYHTYFNIRNNIIGFLRVRTTACAESASWDWRGCGLRQSNKGCADEAI
                     AADNGINCNDNTFFYAPMELGPGNPSTLYFGTEKLYRSSDAGDTMTAVGGPFSQMPGS
                     TLGVPVTAIGISQVDDNLRVVGLNNGKIFRTTTGSTTFDDISGPIPVGTFVTQTIIDP
                     TNDDTAYVTLNGYGLVLGQHIWKTTNLRAGRPTWRPSSTGIPDVPVNDLVVDPKNPEE
                     LYAATDIGMFQSLNGGKNW"
BASE COUNT          344 a          338 c          382 g          344 t
ORIGIN      
        1 atgtctccgc tcaccacctt aaacgcttat gtcaaatttg ctgctgtcaa aactgactca
       61 ggtgtgacga tcgtggcggc aacctccgaa ttcgatacgg tgaaaggttg tggaagcgga
      121 gcgcttgccg ggcggggcgg agtgctcagg cgttcgactg atagcggtgt aacctggtca
      181 gagccagttt caggcatcag cggttattgt ggaggccagt gcttttatga cattgcggtt
      241 gccatgtcac cgcttgaccc aaaagtgatt tatatcggag gccaggggcg agttggtcag
      301 gttggaaatt gtgccagcat tacgtttgcc cgatccggtg atggagctac ctttgcccgc
      361 cgggaacgcg gactctatgt tgatactcac gcgattgagg tctcaactac caacccctct
      421 attgtgtata ccgggaatga cgccggtatc ttcaaatcaa cggatgaagg gatgaactgg
      481 accagtttaa acactccgga tttggccacg atcttgtttc atagtgtagc gttgcaccca
      541 accaaccgaa atctttgtat cgggggaacg caggatctag gaactgaatt tcgaagggcg
      601 gacggaacct ggtctcagac tgacatcagc gacggtgggg cggtggcgat tgatcgcaat
      661 gttcgcgata cctcgaatgt cttgatgtat cacacctatt ttaacattcg aaataacatt
      721 atcggttttc tcagagtacg aaccaccgcc tgtgccgaaa gtgcaagctg ggactggcgc
      781 ggatgtgggc ttcggcaatc aaacaaaggg tgtgccgacg aagccatcgc ggctgacaat
      841 gggatcaatt gtaacgacaa cacgtttttc tacgcaccaa tggagttggg gccaggaaac
      901 ccgagcacgc tgtatttcgg aactgaaaag ctctatcgct cttcggatgc cggagacaca
      961 atgactgcag ttggtggtcc ctttagccag atgcccggtt cgacccttgg ggttccagtc
     1021 acagctatcg gtatttccca agttgatgac aacctgcgcg tggttgggct caataatgga
     1081 aagatatttc gtaccaccac gggctcaacc acatttgatg atatttcagg gccaattccg
     1141 gttggaacct ttgtcacgca aaccattatt gacccaacga atgatgatac agcctatgtg
     1201 accctcaatg gctatggttt agtattgggg cagcacattt ggaagacaac caacctgaga
     1261 gccggacgac cgacctggag accttcttcg actgggattc cagatgttcc agtcaatgac
     1321 cttgttgttg atccaaagaa cccggaagaa ctctatgctg ccactgacat tgggatgttt
     1381 cagtcattga atggaggcaa aaactggg
//
LOCUS       DAOQDO010002016         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110090_length_1407_cov_3.890533, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002016 DAOQDO010000000
VERSION     DAOQDO010002016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110090_length_1407_cov_3.890533"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..1196
                     /locus_tag="PKZ53_24590"
     CDS             537..1196
                     /locus_tag="PKZ53_24590"
                     /inference="COORDINATES: protein motif:HMM:NF015141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43683.1"
                     /translation="MITVSYKRSFVIGMLLAGATSRRTDLPNKLRATFTVVHSLAQTD
                     LAEWKASEVRRLFGLPVQILQNQQATQTSFSFVQEGRLRVIHDWFHRANQKTITDKVR
                     FLSHPIGLAVILSDCGFVHYSNSIGIQLPDINRASTQRLLEHITGFCGATGHLISENN
                     EPGITGVQLIYSDNEARKLWECASPWMPSIPSVVDKFSVFSDFLDIGKDGRHPNGINR
                     G"
BASE COUNT          359 a          326 c          360 g          362 t
ORIGIN      
        1 cctgagcggc acaacctgga cgctggttgg aacgccaaat ggtctgggca gcggcctcgg
       61 ccagttccgc gctccgagca agatccgata aacctttaac ctcaatgttt gattaaggtt
      121 atgggagcct gagtaattca ggctcccatt ttctttgctt ttggaaaaat tagtcgttga
      181 caaacagtcg tttatctgta caatccgacg tttccagtga cgcggggtgg agcagcctgg
      241 tagctcgttg ggctcaagac gcaaaaccct tcccgtcagc cttgatttca tcactaatca
      301 attcaaagct ggcacttaca gacaataacc accgtcattg tctgtgatat ggggggatgt
      361 tcttgcattg ggcggtatgt ggggtaactc acatatgttg acctgtcaaa ttcggggaag
      421 ccactggtgg ggtaatcccg agccaagccc aaaaatgcaa tttatttttg ggaaggtgta
      481 gagactgtca ctgatgttcg aagaggtccg acacgttgat gaaggacata gatcatttga
      541 taactgtttc atataagcgg tcatttgtga ttgggatgct cctggctggt gcgacatcgc
      601 gccgaacaga tcttccgaat aagctgcgcg ccacatttac ggtggttcac agtctggcac
      661 aaactgacct ggctgagtgg aaagcttccg aagtgagacg gttgtttggc ttgccggttc
      721 aaatacttca aaatcagcaa gcaactcaaa caagcttctc ttttgtgcag gaaggccgac
      781 tgagagtcat ccacgactgg tttcatcggg caaaccaaaa aacgattacc gataaggttc
      841 gctttttgtc tcatccgatt ggactggcgg taattctcag tgattgcggg tttgtacact
      901 attccaactc gattggaatt cagcttcccg acatcaatcg tgccagtaca caacggttgc
      961 tcgaacacat cacagggttc tgtggtgcaa ccggccacct tatttctgaa aacaatgaac
     1021 ctggaattac tggagtccag ctcatttatt cagacaatga ggcccgaaag ctttgggaat
     1081 gtgcttcccc gtggatgccg tcaattccct cagtggttga taagttctca gttttcagtg
     1141 atttcctcga cattggtaaa gatggcaggc acccgaacgg tatcaaccga gggtgaagag
     1201 aaagtccaga ccacaaactg attttcaggc agcgaaagct gtagtggtac gcataaccca
     1261 aaggtcgcgg gttcaaatcc cgcccccgca accaatttgc ttcaaagaaa aagccatcgg
     1321 gcaaccgatg gctttttctt ttttggattg agaaaaccag ggttgaagac tttgggcttg
     1381 gggctgaaga cgtcgggctg aagaagg
//
LOCUS       DAOQDO010002017         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110119_length_1407_cov_2.468195, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002017 DAOQDO010000000
VERSION     DAOQDO010002017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110119_length_1407_cov_2.468195"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKZ53_24595"
     CDS             complement(<1..500)
                     /locus_tag="PKZ53_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43684.1"
                     /translation="MRRFTVVATIGVFILPMPSFAQTKPTRPSKVRKDSQLPVLSPQS
                     FNSAEPRTLNGGETHTYPLKLKANDYVKLVVEQRGIDVVVRLVGPDGTPVQEQDSSAA
                     QGPELIPFIAEKGGTYQLEVEALEKDVPPGKYELKLEAHRKATKQDRAEVELENLNKE
                     VSTLED"
     gene            944..>1407
                     /locus_tag="PKZ53_24600"
     CDS             944..>1407
                     /locus_tag="PKZ53_24600"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ43685.1"
                     /translation="MIVAVFNQSGGVGKSTLTRDLGFELFSRGLKVLLIDADPQGTLG
                     FFFGIYPHGCPKTDMFWSGICGSEDPGQPPIAQAECGISLGLANKTLVADEILLMQQH
                     DPARLLSVLEPLKEVYDFILIDCPPRISELTIQILFATDGLLIPVQTEAKSV"
BASE COUNT          326 a          342 c          326 g          413 t
ORIGIN      
        1 tcatcttcca gggtacttac ttccttattc agattttcca gttcaacctc agctcgatcc
       61 tgtttagtag ccttccggtg tgcttccagc ttgagttcat actttccggg cggcacgtct
      121 ttttccaatg cttcaacttc aagctggtag gttccacctt tttctgcaat gaatggaatc
      181 aactctgggc cttgagcagc ggaactgtct tgttcttgca ctggtgttcc atccgggcca
      241 accagccgca ccaccacatc aattccgcgt tgctccacga ccagtttcac atagtcgttg
      301 gctttgagct tcaacgggta ggtgtgtgtt tcaccgccgt tcagggttcg aggttcggca
      361 gaattgaaag attgagggct gaggactgga agttgcgagt ctttccgaac tttagatggt
      421 ctggttggtt tggtttgggc aaaggatggc atcggaagga tgaagactcc aatcgtcgcc
      481 accaccgtga agcgtctcat ccatttcatc atcattggtc tccccttgat aagtaccttc
      541 gatttcctga catttaacca cagaaaacat gggaacctcg gaaaaaatca agcacttccc
      601 aaatccaatg tctcaggttt gccatcattt gacaaaatct ccttccaggt tgaactttcg
      661 cttgtttggt agcctgatca accctgggtt cgaaacaggc cacgagtatc agtttcagac
      721 aagccggttt ttcgtgcgtc ccgttcgttg cgtctcacac ctcaagacac ccgattgaac
      781 aaaaattcat ttttgaaatg cgattgaaat ttgcttgacg aatctctgtg acgagcaata
      841 gggtaatttt tcttcctcaa gaaccgaatc cttttatgtt tgcaggcata tctggcccgg
      901 cattggaaaa attcggtttg actcacaagg agatggaaac actatgatcg ttgccgtttt
      961 taatcagtcg ggtggagtcg gaaaatcaac ccttacccgt gatctggggt ttgagctttt
     1021 ttcacgtggg ctcaaggtac tgctcattga tgcggaccca cagggaaccc ttggattttt
     1081 cttcggaatt tacccgcatg gttgcccaaa aaccgatatg ttttggtcag gaatttgtgg
     1141 ttctgaagat cccggccagc caccaattgc tcaggccgaa tgtgggatca gtttgggact
     1201 ggccaataaa acgctggtgg cagatgaaat tcttctgatg cagcaacacg acccggcccg
     1261 cttattgagc gtacttgaac cgctgaaaga agtgtatgac tttattttga ttgattgtcc
     1321 acctcgcatt tcagaactga cgattcaaat tctgtttgca accgatggtc ttttgattcc
     1381 agtccagacc gaggccaagt cggtcat
//
LOCUS       DAOQDO010002018         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110228_length_1406_cov_3.059215, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002018 DAOQDO010000000
VERSION     DAOQDO010002018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110228_length_1406_cov_3.059215"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1406)
                     /locus_tag="PKZ53_24605"
     CDS             complement(<1..>1406)
                     /locus_tag="PKZ53_24605"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNJ43686.1"
                     /translation="EKETIHIAKQMCAGLHAAHLQNIVHRDLKPENVMIQQLSDGEIM
                     ARVLDFGIAKLLQRPGEIETESLTGAALPGTPKYMAPEQMFGGNVDARVDVFAIGLII
                     YEMLTQTLPLVLMSNVKPLTELRPDITPRLNDIVLKAISKEVDDRFESAHAFKQALEE
                     IEQHAFIEAELVKRTRTLGELAGSLPTSTQRVTVANRVVSQTDPNVGSTMRIEVAPPP
                     PKGKAWIAIPVILVLLGGGFGGLWAFSPDFRKLFKSETATAEPGGKLAEQFIPQLVSA
                     RSGVTKIGSMTANKASGYGFLSQPEHSTTIDEFQVSKQLVTSAQYAEFVRLAKHRPPS
                     NWNGPTPPSDLVNKPVTMVSWEDCQDYCSWLTVQTNRLHRLPTEFEWEYIARDGGHFG
                     TSDMLTGYREWTGSTIGPYPGAKWDTSILQDFASARIIRGETVDPDGKQVDQLTYRSF
                     QVPAYAESDKLGFRVVCET"
BASE COUNT          325 a          358 c          372 g          351 t
ORIGIN      
        1 gtctcacaaa caacacgaaa tccaagttta tcgctttcgg cataggctgg aacctgaaat
       61 gagcggtagg ttaactgatc aacctgtttc ccatccggat caaccgtttc gcctcgaatt
      121 atccgggcac tggcaaagtc ttgcaaaata ctggtatccc atttggcacc agggtatgga
      181 ccaatcgttg aacccgtcca ttcccgatat ccggtgagca tatccgaggt gccaaaatgg
      241 cctccatcgc gggcaatata ctcccattca aattcagttg gtaatcggtg gagccggttg
      301 gtttggaccg tgagccagga acagtaatcc tggcaatctt cccaactcac catcgtaacg
      361 ggtttgttta cgagatcgct tggtggggtg ggaccattcc agttggatgg cgggcgatgt
      421 ttggccaatc ggacgaattc agcatactgt gcactggtca ccagttgttt tgagacctgg
      481 aattcgtcaa ttgttgtgga atgctctggt tgggataaaa aaccgtatcc actggcttta
      541 ttggcggtca tcgagccgat tttggtcaca ccactccggg cagaaaccag ttggggaata
      601 aattgctcgg cgagtttgcc gcctggttca gccgtggccg tttccgattt gaacagtttg
      661 cggaaatcag gtgaaaatgc ccacaggccg ccaaacccac cgcccaacaa caccagaatc
      721 accggaatcg cgatccaggc ttttcctttt gggggcggag gtgcaacctc aatccgcatg
      781 gtggaaccaa cgtttgggtc ggtttgagaa acgacccggt tggcaacggt tacgcgttgg
      841 gttgaggtcg gaagggaccc ggccagttca cccagggttc tcgtccgttt aaccagctcg
      901 gcttcaataa aagcatgctg ctcaatttct tcaagcgcct gtttaaaggc atgggctgat
      961 tcaaaccggt catctacttc tttggaaatc gctttgagga caatgtcatt gagccggggt
     1021 gtaatatctg gtcggagttc agtcagcggt ttgacatttg acatcaacac cagcggcagc
     1081 gtctgggtca acatttcata aatgatcaat ccgatggcga acacatccac ccgggcatca
     1141 acattgccgc caaacatctg ctcaggggcc atgtacttgg gggtcccagg aagggcagcc
     1201 ccagtcaacg attcagtttc gatttcacct ggccgctgga gcagtttggc aatgccgaaa
     1261 tccagcacgc gcgccatgat ttcaccgtct gaaagctgct gaatcatcac gttttcaggc
     1321 ttgagatcgc ggtgaacgat gttttgcagg tgcgcagcat gcagcccggc acacatttgt
     1381 ttggcaatgt ggatggtttc tttttc
//
LOCUS       DAOQDO010002019         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110440_length_1405_cov_2.442963, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002019 DAOQDO010000000
VERSION     DAOQDO010002019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110440_length_1405_cov_2.442963"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKZ53_24610"
     CDS             <1..834
                     /locus_tag="PKZ53_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43687.1"
                     /translation="ELRIGRSVVILDNADRKLTSPKLAEILTSQWTTDRLLGKSEMLT
                     LPNFGIWILTANNPQFSDELNRRRVRIRLDTHLESAELRQEFKHQDLLGYIQSNRACL
                     VSGLLTLIQAWVVAGSPLRESRQLPSFEAWSSVMAGILNTCGIDEFLANNIEVSGHTD
                     SSEEEWGLFFADWFAVNRDRPFLPGDVVDFCLNTDPHLLNEVRGGGTSRSQAICLGKA
                     LPRKVGCSHGKLKLIQSSVIRSNGKGNRAGYRIVPTADAPPSPHQPTTHQEMVDNFSP
                     D"
     gene            1010..>1405
                     /locus_tag="PKZ53_24615"
     CDS             1010..>1405
                     /locus_tag="PKZ53_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ43688.1"
                     /translation="MIITVGGIKGGSGKTTIATHLAILRATAGKDVVLVDADDQETAS
                     DFTLLRNERSQGEAGYSCIKLNGPAVRTEVLRIKSKYDDVIIDTGGRDTTSQRAALTA
                     SDLLLVPFVPRTFDIWTVEKVVNLVEELRP"
BASE COUNT          355 a          343 c          353 g          354 t
ORIGIN      
        1 gaactgcgga ttggccggag tgtggtcatt ctggacaatg ccgaccgcaa gctgacgagc
       61 ccgaagttag cagaaattct gacctcacaa tggacgacag atcggttgct tggaaagtct
      121 gagatgttga cactgccaaa cttcggaata tggattctga cagccaataa tccacaattt
      181 tcagacgaac tgaaccgacg acgagtccgg attcgcctgg atacgcatct tgaatcagca
      241 gaacttcggc aagaattcaa gcaccaggac ctgcttggct atatccagtc caatcgagcc
      301 tgcctggtca gtggtcttct gactttgatc caagcctggg ttgtggctgg atcaccgttg
      361 cgtgagtcgc gacaactgcc ctcattcgaa gcctggtcat cagtcatggc tgggattctc
      421 aatacctgtg gaattgatga gtttctagcc aataacattg aggtctcggg tcatactgat
      481 tccagtgaag aagagtgggg cttgtttttt gctgattggt ttgcagttaa tcgggatcgt
      541 ccatttcttc caggggatgt cgttgatttt tgcctcaaca cagaccctca tttgctcaac
      601 gaggtacgag gtggtggcac cagccgatcc caggcaattt gtcttggaaa agcacttcct
      661 cgaaaagttg gctgttcaca cggcaagttg aagctgattc agtcctctgt catccgctcc
      721 aacggcaagg gcaatcgagc aggataccgc attgttccga ctgctgatgc ccccccatcg
      781 ccacaccaac caaccactca tcaggaaatg gtggacaact tctcaccaga ttaaaactcc
      841 catctggtcg gctcaggatt tgctcctggc cgacctcttt cttctatctt ttaaaaaaag
      901 gaagtcactt tcccttgaat caaactacca ggttaactat actttaatac cgttcacata
      961 tctttttcat accttactga gattatccag gtactatatt gaggttaaaa tgattatcac
     1021 agttggagga atcaaagggg ggtccggcaa aacaaccata gcgacccatc tggcaatact
     1081 gcgagccaca gccggaaagg atgtggtgct ggtggacgcc gacgatcagg aaaccgccag
     1141 tgatttcacg cttcttcgca atgaacgcag ccagggggaa gccgggtata gctgtatcaa
     1201 gttaaacggc ccggctgtgc ggactgaggt tttaagaatt aagtcaaaat atgacgatgt
     1261 gattattgac actggtggac gtgacacaac cagccagcgg gcggcgctta cggccagtga
     1321 tctgttgttg gtgccgtttg tccccagaac ctttgatatc tggaccgttg agaaagtcgt
     1381 gaatctggtg gaagagttac ggcca
//
LOCUS       DAOQDO010002020         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110532_length_1404_cov_3.595997, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002020 DAOQDO010000000
VERSION     DAOQDO010002020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110532_length_1404_cov_3.595997"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..875)
                     /gene="hxpB"
                     /locus_tag="PKZ53_24620"
     CDS             complement(159..875)
                     /gene="hxpB"
                     /locus_tag="PKZ53_24620"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HNJ43689.1"
                     /translation="MFQAVIFDLDGLLIDSEPLWRDTEVKVFGALGLPITHQDCEQTI
                     GLRVDEFVRYWYDRFPWKGKSLQVVEQEIVEEMIFCIRQRGVPKAGVEKIFNFFAEKQ
                     LPMAVASSSYVRIIEAALQTLGLLPHITLFHSAEHETFGKPHPAVYLTTARKLNVDPV
                     RCLAFEDSPMGVQSAKSAGLTCVCIPDSIVDQTKLSQADLLLESLEVFNDEHLTALAR
                     AEEVGLKKPGLKKPGLKKSG"
     gene            994..1257
                     /locus_tag="PKZ53_24625"
     CDS             994..1257
                     /locus_tag="PKZ53_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43690.1"
                     /translation="MQYFVLYIAVRNDNLKSITLYVTAIEYQQIRAEADQRGISISQL
                     MRLLINEHSSLALPPLPPRGAPRGDRNGKRKSTKKNTPDLEST"
     gene            1260..>1404
                     /locus_tag="PKZ53_24630"
     CDS             1260..>1404
                     /locus_tag="PKZ53_24630"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNJ43691.1"
                     /translation="MNYIGSKLSLLPFLEQVFREISDGTEQTVCDMFAGTGAVGRHFK
                     KLGL"
BASE COUNT          383 a          352 c          323 g          346 t
ORIGIN      
        1 cagggctcag ggcttagggc tgaagactcg caagctcggg gctgaagtaa gcgggctgaa
       61 gaaaccaggg ctcagggctt agggctgaag actcgcaagc tcggggctga agtaagcggg
      121 tttaataccc cgagcccgct ttcttcagcc cgactttttc agcccgactt cttcagccct
      181 ggtttcttca gccctggttt cttcagcccg acttcttcag ctctggctaa tgccgtcaaa
      241 tgttcatcat taaagacttc aagtgattcc aggagcaaat cggcctgact gagtttggtc
      301 tggtccacaa ttgaatcggg gatgcagaca caggtcagcc cggcactctt tgcggattga
      361 acccccatcg gagagtcttc aaatgccaga caccgcaccg gatcaacatt gagcttccgg
      421 gcggtggtca gatacaccgc tggatgaggt ttgccaaagg tctcgtgctc ggctgagtga
      481 aacaaggtaa tgtgcgggag caatcccaat gtttgcaggg cagcttcgat aatccgcaca
      541 tatgacgacg aagcaacagc cattggtaac tgcttttcag caaagaagtt aaatattttt
      601 tcaacacccg ccttcggtac cccgcgctgc cggatgcaaa aaatcatttc ctcaacaatt
      661 tcctgctcaa ccacctggag cgacttgccc ttccagggaa atcggtcata ccagtagcga
      721 acaaactcat caacccgcag accaatcgtc tgttcgcaat cctggtgggt aatcggaagg
      781 ccgagcgctc cgaagacttt gacttccgta tcccgccaga gcggttcaga gtcaattaac
      841 aatccatcaa gatcaaaaat tacagcttga aacatagttt ttaagcaaca ggaactcttt
      901 tgtttccttt aaggaatagt ttgctattga tacagtttag acactttatt cgatcaaaac
      961 ccaaccaata cacagtaatt ttccgtttac aaagtgcagt actttgtatt atacattgcc
     1021 gtgcgaaacg ataacctgaa aagtatcaca ctctatgtga ccgcaattga atatcaacaa
     1081 attcgcgctg aggccgatca gcgggggatt tccatttcac aattgatgcg gttgctgatc
     1141 aatgaacaca gttcgttggc acttcctcca ttacccccac gaggcgcccc tcgtggagat
     1201 cgaaatggaa aacggaaatc tacaaagaaa aatactcctg atttggaatc tacataacta
     1261 tgaattacat cggctcaaaa ttgagcttac taccctttct tgaacaagtc tttcgggaaa
     1321 tctcggatgg gacagaacag acggtgtgcg atatgtttgc cggaactgga gctgtgggcc
     1381 gacatttcaa aaaattagga ctga
//
LOCUS       DAOQDO010002021         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110621_length_1404_cov_1.925871, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002021 DAOQDO010000000
VERSION     DAOQDO010002021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110621_length_1404_cov_1.925871"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1404
                     /locus_tag="PKZ53_24635"
     CDS             <1..>1404
                     /locus_tag="PKZ53_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ43692.1"
                     /translation="LPKGVSLIHFYDRTELIDRTIRTVITNLVEGALIVIFVLILLLG
                     NWRGALLVATVIPLSMMSAAIGMNFFGVSGNLMSLGAIDFGLVVDGAVIMVENAIRRI
                     GERQLEQGRTLTPGELRATICNAAAEVRKATMFGELIIAVVYLPILSLTGIEGKMFKP
                     MALTVIFALAGAMILSLTYVPAAMTLIFRGPVSERENSLIAIAKRWYRPALDHVIRYR
                     SQALALATGLILVAAAVFPLLGAEFVPRLDEGSLAIQVQQLPSVSLTQSIQTTTEVER
                     VLRQFPEVTRVVSKTGRAEVATDPMSVDFSDLYIALKPPAEWKSARDRETLIALMSKQ
                     LENQVPNAQFSFSQPIELRVSELISGVRSDVVIKLFGDDLDILKKKANEIARVVGGIQ
                     GAEDIKVEATTGLPQLQVVPRREVIARHGINVEDVNTLVESIVVGKEGGLVYEGEQRF
                     GLVVRLDPAMVRDVQAIR"
BASE COUNT          302 a          357 c          418 g          327 t
ORIGIN      
        1 cctgcccaaa ggcgtatcgc tcattcattt ttatgaccgc acagaattga ttgaccgaac
       61 catccggacg gtcatcacca acctggttga aggcgccttg attgtgattt tcgtgctgat
      121 cttgctgttg gggaactggc gcggggcgtt gctggtggca accgtgattc cgctctcaat
      181 gatgtcggcg gccattggca tgaatttctt tggcgtgtcg ggcaacctta tgagccttgg
      241 ggcaattgat ttcggcttgg tagtggacgg cgcggtgatt atggttgaaa acgctattcg
      301 gcgaattggc gaacgccagc ttgaacaggg acgaacgctc acaccgggcg aacttcgtgc
      361 gacgatctgc aatgcggcgg cggaagtccg caaggcaact atgttcggtg aactgataat
      421 tgccgtggtc tacctgccga ttctgagcct gaccggaatt gagggaaaaa tgttcaagcc
      481 gatggcattg acggttattt tcgccctcgc cggagccatg attctctcgc tcacctacgt
      541 gcccgctgcc atgacgctga tctttcgcgg tccagtctcg gaacgcgaga actcgctgat
      601 tgccattgcc aaacggtggt atcggccagc gctcgaccac gtaattcgtt atcgctcgca
      661 agcactcgca ctggcgactg ggctgatact tgttgcggca gcggttttcc cgctccttgg
      721 ggcagaattt gtgccccggc tggatgaggg ttcattggcc attcaagtcc agcaactgcc
      781 aagtgtttcg ctcacccaat ccattcagac gacgaccgag gtagaacgtg tcctgcggca
      841 attcccggaa gtaaccaggg tggtttccaa aaccgggcgg gcagaagtcg ccaccgatcc
      901 gatgagtgtg gatttcagtg atctctatat tgccctgaaa ccacccgctg aatggaagag
      961 cgccagagac cgagaaaccc tgattgcgct catgtcaaaa caactggaaa accaagtacc
     1021 caatgctcag ttcagtttct cgcagccaat tgagctgcgg gtctcagaac tgatttctgg
     1081 cgtgcggtcg gatgtggtca tcaaattgtt tggcgacgat ttggatatcc ttaagaaaaa
     1141 agcgaatgaa atcgcccgag tcgtgggtgg aattcaaggc gcggaggata tcaaagtcga
     1201 agccaccacc gggttgcctc agttgcaagt ggtgccgcgc cgtgaggtga ttgcacggca
     1261 cggcatcaat gtcgaggacg tcaacacgct tgttgaatcc attgtggtgg gcaaagaagg
     1321 cggcctggtc tatgaagggg agcagcgatt cgggctggtg gtgcggcttg acccagcgat
     1381 ggtgcgggat gtgcaggcta tccg
//
LOCUS       DAOQDO010002022         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110760_length_1403_cov_2.033383, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002022 DAOQDO010000000
VERSION     DAOQDO010002022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110760_length_1403_cov_2.033383"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKZ53_24640"
     CDS             <1..344
                     /locus_tag="PKZ53_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43693.1"
                     /translation="FNSPLGLERLHFWMIGIQLFRHLLRQKQAQLFLYLPPGCPISTG
                     RNFYSIQALLDWILAEALSLAEKFDFRNGNDFYQRQLNQLPEFEVTQVPIPLPSKPQS
                     ADSRRQTLPSL"
     gene            complement(407..616)
                     /locus_tag="PKZ53_24645"
     CDS             complement(407..616)
                     /locus_tag="PKZ53_24645"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ43694.1"
                     /translation="MDNKEYLTIEESAEVLGVDPSTVEKLVQDGVFRAVRIGVERRIG
                     RSYIEAFRSGRIRAPRKKYPPHSVE"
     gene            940..>1403
                     /locus_tag="PKZ53_24650"
     CDS             940..>1403
                     /locus_tag="PKZ53_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43695.1"
                     /translation="MTHNFDPKDLMASTPVESPPTSGEPQLPTITPDQPGSLPRPAAG
                     PPHLTAHHFNQLKKPASANLIDLARETAVTGEFQALPENFDELPTDPLPSLEHIIFGP
                     RWRYLPSPVGPEPRPDSVTFSAHLPSGFEVLAARVRGKKHKHEGTHCDDWFE"
BASE COUNT          349 a          393 c          310 g          351 t
ORIGIN      
        1 ttttcaattc tccacttgga ctggaacgac ttcatttttg gatgatcggg atccaacttt
       61 tcagacacct gcttcgccag aaacaggctc aactgttttt gtatctgccc ccaggctgcc
      121 caatttcaac cggaagaaat ttttattcaa ttcaagcact tctggattgg atactggccg
      181 aagcactcag tctggcagag aaatttgatt ttcgaaacgg caatgatttt taccagcgcc
      241 agctcaacca gcttcccgaa tttgaggtca ctcaggtccc cattcccctg ccatcaaaac
      301 ctcaatcagc cgattccagg cgtcagacgc tcccttccct gtaaattttc agaatcaaaa
      361 acctgactgt gaacactggc cggaactatt tatgctggaa acctgactat tcgaccgagt
      421 ggggtggata ttttttgcgt ggggctcgaa tccgacctga ccgaaaggct tcaatgtaac
      481 ttcggccaat tcggcgctca acaccaatcc gcacagctcg aaaaaccccg tcttgaacca
      541 gtttttcgac tgttgacgga tcaaccccaa ggacttcggc actttcttcg atagtcagat
      601 attccttgtt gtccatgata gttccaggtt ggtgactgat cgtgaccaga gtgtgtggag
      661 gtgaattgaa caattgaccg gtacgagcct gtggcaattt ctatgtttaa cggactgggg
      721 ctggttgtca agcaacatca ggctgaacct gaaagatgta ccgatttgaa ctcaagaagg
      781 cgagcctggc gtcagtgcgg cgttttctct tgcgtatgcg gatcctcaga acgtcaaaac
      841 cgaactgggt tcctttcact tgccctaatc ttgtagtaaa aaagagatta gccaccgcag
      901 gaaacggaaa cgtccattct tggtcttcag ggtactttca tgacacacaa cttcgatcca
      961 aaggatttga tggcttccac accggttgaa agtcccccaa cttcgggtga gccacaactc
     1021 cccaccatca ctccagatca acccggttca ctcccacgcc cggcagccgg tccgccacac
     1081 ctgacagccc atcatttcaa tcaactcaaa aaacctgcca gcgcaaacct gattgattta
     1141 gcgcgtgaaa cagccgtcac cggcgagttt caggctttgc cggaaaactt tgatgaactt
     1201 ccaacggacc cgcttccatc gctggaacac atcatctttg gtccccgatg gcgatacctt
     1261 ccctcacctg tcggccccga gccaagaccg gattccgtaa cgttttcagc ccatctgccg
     1321 tctgggtttg aagtactcgc cgccagggtt cgcggcaaaa aacacaaaca cgaagggacc
     1381 cattgtgatg actggtttga gtt
//
LOCUS       DAOQDO010002023         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110874_length_1402_cov_2.503341, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002023 DAOQDO010000000
VERSION     DAOQDO010002023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110874_length_1402_cov_2.503341"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..665)
                     /locus_tag="PKZ53_24655"
     CDS             complement(117..665)
                     /locus_tag="PKZ53_24655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNJ43696.1"
                     /translation="MPNTVTILLQKWRSGDQAALDELIPIIYEKLHLLAASYIRGQKN
                     DYTLQPTALINEAMIQLLDDCTNFENRFHFIAIVAKTMRRVLVDYARRENAKKRGRDY
                     YSISLTGLNPSILPQNIDVITLHEALNRLEQIDPLQMKIVELRYFCGLTIKETAEVLD
                     TSTTTVSNEWAMAKTWLFMELN"
     gene            1210..>1402
                     /locus_tag="PKZ53_24660"
     CDS             1210..>1402
                     /locus_tag="PKZ53_24660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43697.1"
                     /translation="MFTLLCRNRLIPELVTSLFKVFACPKRWVMASAVVAGLVWFSTP
                     TLAQSAIDEFNPNANNQVTT"
BASE COUNT          337 a          358 c          313 g          394 t
ORIGIN      
        1 gtcaggatgg tgtcgtgggt acacgactca agctcggccc ttcagggccg agtctattga
       61 ccaaattctt cggtgtgatt caagctccgg cggtcaccgc cggggtctgg ttgaccctaa
      121 tttaactcca taaacagcca ggttttggcc atggcccatt cgtttgacac cgtggtggtc
      181 gatgtatcca aaacctcggc ggtttctttg atcgtgagac cgcaaaaata ccgcaactcg
      241 acgattttca tctgaagggg atcaatttgt tccaggcgat tgagggcttc atgcaaggtg
      301 atgacatcaa tattttgagg caggatactt gggttaagtc cagttaagga aatactgtag
      361 tagtcccgcc ccctcttttt cgcattctcc cggcgggcat aatcaaccaa tacccgccgc
      421 atggtttttg ccacgatggc gataaaatga aagcggttct cgaaattggt gcaatcatca
      481 agcagttgga tcatggcctc attaatcaag gctgttggtt gtaaggtata atcatttttt
      541 tgtcccctga tatatgaggc cgccagcaaa tgaagttttt cataaatgat tgggattagt
      601 tcatccaggg cggcttgatc gccgcttctc catttttgga ggagtatggt cacggtattt
      661 ggcattcagc ctctccttga acagtatcga aagtgcctca gcgattatac ctttcgtgac
      721 cttgactgtg ccaaccggaa tgaggtttcc aaccctcaat gttaaagatc agggattggt
      781 caacctcttg tgaagactga acttttccct ggctggagtg atcctgaatc aggtcgggat
      841 cacaaacatg cccgatcaat atggccaatc ccgccttcat cacatttgcc caggtgctca
      901 tcacgcctcc aaagccatct ccagagttct tttcttccca acaaggctca gcgccctcag
      961 gaaccatctc ggtaacctct tgaaactaaa gcaataaaaa aattgtgaaa aaatttttgg
     1021 aaatagcttt gtaaaaaatc cctcctttac gacttatagc ccattgattc tcattgctcg
     1081 gtcaaaaaac agtttccctg atattccagc aaatctaaat gaactcctgg gcttttgtgt
     1141 tcagaaagtc cgccgcattc cccaatcctg cgcagtggtt ccgcgctcca atcctttaag
     1201 aggtccccca tgtttactct tctttgccgc aaccggttga tccccgaact tgtcacctct
     1261 cttttcaagg tattcgcctg ccccaaacgc tgggtgatgg cgtcggcagt tgtggctggt
     1321 ttggtgtggt tctctactcc gacattggcg caatcggcga tagatgagtt taaccccaat
     1381 gccaacaacc aagttacaac ct
//
LOCUS       DAOQDO010002024         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110912_length_1402_cov_2.153675, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002024 DAOQDO010000000
VERSION     DAOQDO010002024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110912_length_1402_cov_2.153675"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PKZ53_24665"
     CDS             complement(<1..1387)
                     /locus_tag="PKZ53_24665"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43698.1"
                     /translation="MALRLFSPDGKEVDTSDEPNGNQGFERVKWISAEAGTYELRIEA
                     TDSAAAAARCLVRFEALRPATELDRSVMEIDHLNEQVQRLFLEGKIDTALEVMLKGLA
                     LAEKNLPPDSEMLAQCLNNLASLYFTKGEFAKAEPVYERALAVYRQAGLQESQDALTL
                     LQNLGTLYQELGKYNLAQPAFEQLVDLRKKVLGPDHPEVALALNRLAMLYRVQGQLDQ
                     IEPLLQQALSINEKALGPESLGVAASFNSLGMYYFSRGDLTQAVQVFERSIAIMRKGL
                     PKNHPDFLTSYNNLAIVNDQLGNFKIAADYINQALAQAEAQFGPDHPAVALLLSTLAT
                     NRRTVGDFHQAQLYDQRAYEIRLKLFGENHKETATSLNNIGFTYYKRGDYESAEPYYR
                     RSLAIREKVLPPNHVEIGHSYTNLAALLIAKKDYQPAKTYQEKGLQILEAALGPNHPD
                     VANSLNNLGILY"
BASE COUNT          327 a          357 c          358 g          360 t
ORIGIN      
        1 catagagaat ccccaggtta ttcaaactgt tggcgacatc tggatggttt ggcccaagcg
       61 cggcctcaag aatctgcaac cctttttcct gatatgtttt ggctggttga tagtcttttt
      121 tggcaatgag taaagctgcc agattggtgt aactatggcc aatttcaaca tgatttggcg
      181 ggaggacttt ttcccggatg gccagggacc gacgatagta cggttccgca ctctcataat
      241 cacctcgctt gtaataggta aagccaatgt tattcaaact ggtggcagtt tctttatggt
      301 tctcaccaaa gagcttgagt cggatttcat acgcccgttg atcatagagt tgcgcctgat
      361 gaaaatcgcc cacggttcga cgattggtgg ccagtgtact gagcagcaac gccacggctg
      421 gatggtcagg accaaactgg gcttcagcct gcgccagggc ctgattgata tagtcggctg
      481 cgattttgaa gttccccaac tggtcattca caatggccag attgttgtaa gaggtcaaaa
      541 aatcagggtg gttttttggt aaccctttgc gcatgatcgc aattgatcgt tcaaacacct
      601 gaacggcctg tgtcagatcc cctcgtgaga aatagtacat ccccaatgaa ttgaagctgg
      661 cggcgactcc caaagactca ggccccagtg ctttttcatt gattgaaagc gcctgttgca
      721 ataacggctc aatttgatcg agttgcccct ggacacggta caacatcgcc agccggttga
      781 gcgctagggc cacctcggga tggtccgggc caagcacttt tttgcgcagg tcaacgagtt
      841 gttcaaaggc aggctgggcg agattgtatt tcccaagctc ctgatagaga gtgcccagat
      901 tctgcaacag ggtcagggca tcttgtgatt cctgcaaccc agcttgacga tagacggcca
      961 gggcgcgctc atacaccggt tcggctttgg caaactcacc tttggtaaaa tagagtgacg
     1021 ccaggttgtt caaacattga gccagcattt cagagtccgg cggcaggttt ttttcagcca
     1081 atgccagccc tttcagcatg acctccagag ctgtgtcaat tttcccttcg agaaacaggc
     1141 gctgaacctg ttcattcaga tggtcaattt ccatgaccga cctgtcgagt tcagtagctg
     1201 gacgtagtgc ttcaaatcgc acaagacacc tggcggcagc ggcggctgag tcagtggctt
     1261 caatacgaag ctcataggtg ccagcctcgg ctgaaatcca tttcacgcgt tcaaacccct
     1321 gattgccatt gggttcatct gaggtgtcta cttcttttcc atccggggaa aacaaccgaa
     1381 gcgccacatc aatcccctgc tg
//
LOCUS       DAOQDO010002025         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_110933_length_1402_cov_1.947290, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002025 DAOQDO010000000
VERSION     DAOQDO010002025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110933_length_1402_cov_1.947290"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1402
                     /locus_tag="PKZ53_24670"
     CDS             <1..>1402
                     /locus_tag="PKZ53_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43699.1"
                     /translation="KVLAELKIEALHEAPGQWEADETYFSITLNGQRNCRVEISPVLR
                     KIEHRTIQHPEEGGCYTAHLDLAETLDAGRLLTPVDLSPLWQTDLGATLLSRRKELFR
                     LLQKQESRGLIETADWTSDLSERARSYAKAYREVLTQCDTPEILWLALRLDTIFLEIN
                     NNQETETAVLVQPTHPMRVVWYAAYVDLLGHWEKELGKLKIPRERQRQIDLALIERVT
                     PLNWPAFVLNPGGEIYLFAQNLHFFWALALPVGIPDPGRQAAQIARMVGFQEDEASLS
                     DLPPKRIAAELNAYLGVHPYLKKLRVNAINPGSGGFLAEALREFYAVDQNEQAAEADL
                     PASRPHLEVIAHAHPPLPVKLEALTQLQSDYYESQPRGHRSHLNPLFSIAIRPIDQVC
                     QVPGGDVNLSIAIDQLIPQITLFPPSEYEDSASFYGLLVRFLPYFEKNGPSSRWVHRL
                     SFLPEIRLTRHPVQMYT"
BASE COUNT          342 a          381 c          344 g          335 t
ORIGIN      
        1 caaagtgctg gctgaactca aaattgaagc gctgcacgaa gcccctggcc agtgggaagc
       61 ggatgaaacc tacttctcaa ttaccttaaa cggacagcgg aattgtcggg ttgaaatcag
      121 cccggtgctt cgcaaaatcg agcaccgaac gattcaacac cctgaggaag gtgggtgcta
      181 taccgcccac ctggacctgg ccgaaaccct tgacgcggga aggttattga ctccggtgga
      241 tttgtcgccc ctgtggcaaa ctgacctggg ggcaaccctg ctttcacgtc gcaaagagtt
      301 gttccggttg ctccaaaaac aggaatcccg tggactgatc gaaacagcag attggacatc
      361 tgatctctca gaacgcgccc ggtcttatgc caaagcgtac cgtgaagtct taacccagtg
      421 tgacacgcca gaaatcctct ggctggcact acgactcgat accatttttc tcgaaataaa
      481 taacaatcag gaaactgaaa ccgctgtgct ggtgcaaccg acgcacccga tgcgggtcgt
      541 gtggtatgcc gcttatgttg acttgcttgg tcactgggaa aaagaactgg gcaaactgaa
      601 aataccgcgt gaacgccagc gccagattga tctcgcgctg attgagcgtg tcaccccgtt
      661 aaactggccg gcctttgttt taaacccagg tggcgaaata tatttgtttg cccaaaacct
      721 gcattttttc tgggccctgg cccttccagt tgggattccg gatccagggc gacaggcggc
      781 gcaaattgcc cgcatggttg gatttcaaga agacgaagct tcactttcag atcttcctcc
      841 caaacgcatt gcggcagaat taaacgccta tctaggtgtt catccttatt tgaaaaaact
      901 ccgggtaaat gccattaacc ctggctccgg agggttttta gctgaagccc tgcgtgaatt
      961 ttacgcggtt gatcaaaatg aacaggcagc agaagccgat ctaccagcca gtcgtccgca
     1021 tctggaggtc attgcccacg ctcatccgcc actaccagtc aaacttgaag ccttaaccca
     1081 gcttcagagt gactattatg aatcccagcc acgagggcat cgcagccatc tcaatccatt
     1141 gtttagcatc gccatccgcc caattgacca ggtttgccag gtgcccggtg gggatgtcaa
     1201 cctttcgatt gccattgatc agttgatccc gcaaatcacg ctgtttccgc catccgaata
     1261 tgaagacagt gcgtcctttt atggattgct ggtccggttt ttgccctatt ttgaaaagaa
     1321 tgggccgtct tcccgctggg ttcaccggtt aagctttctc ccagaaataa gactgaccag
     1381 gcatccggtt cagatgtata cc
//
LOCUS       DAOQDO010002026         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111036_length_1401_cov_2.554978, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002026 DAOQDO010000000
VERSION     DAOQDO010002026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111036_length_1401_cov_2.554978"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKZ53_24675"
     CDS             complement(<1..697)
                     /locus_tag="PKZ53_24675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ43700.1"
                     /translation="MSNDPTQSFKPPFRCLNQRNLTRQVVFLFIVVFCAGWGWQLTPV
                     PIVSSFSQTGEKQSSKQTDDEPILRLRSDLVTVNVTVSDKLGRFVTGLKKTDFQVYEN
                     GVLQSIEYFSDIDGPISVGIIFDVSYSMRHSIDARKEEVRAFVESCLEGDEFFLVTFN
                     NKVQQVSDFTSSGETIVNTVALTKTGGQTALYDAVYFGLEKLRNARHPRKALIVLSDL
                     DDNSSRYSLKELRK"
     gene            complement(694..>1401)
                     /locus_tag="PKZ53_24680"
     CDS             complement(694..>1401)
                     /locus_tag="PKZ53_24680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43701.1"
                     /translation="HSYLEVLLGFGSVIWLDAGTTWIVKNTRFDQFEMELETGSSIIE
                     FPGYEQIKLNPTIVLAQTKLKLQLEGRYRFETTGEQRGRCLVYKGEVQNGVTGPGRHG
                     APKQLVFKSGQLLESEISKPPEDVFYKWTRERARILGAYFDQVYTSRKGDSLIDGYSG
                     LWGYGNAPLSNAIWVWSPSRSCYGFYCRNVSGQTESRFVELEYVWRPRYYLYRNRWVK
                     QQRSLLKNKIQSSGKTL"
BASE COUNT          350 a          368 c          303 g          380 t
ORIGIN      
        1 gctttcgcaa ttccttgagc gaataccggc tgctgttgtc atccaaatca ctcaggacaa
       61 tcagtgcttt tctgggatga cgggcatttc tcagcttttc caatccaaag tacaccgcat
      121 catacaaagc cgtctgtcct ccggttttgg tcaatgccac cgtattgaca atggtctcgc
      181 cgcttgaggt gaagtcactg acctgctgga ctttgttgtt gaacgtaacc agaaaaaatt
      241 cgtctccctc cagacacgat tcaacaaaag ctcgaacctc ttctttcctg gcgtcaatgc
      301 tatggcgcat gctgtaggaa acatcaaaga taattccaac ggaaatcggt ccatctatat
      361 cgctaaaata ttcaattgat tgcaggacac cgttttcata aacctggaaa tccgttttct
      421 tcagtccagt gacaaaacgc ccgagcttgt cgctcaccgt gacattgacc gtgaccagat
      481 cacttcgcag ccgtagaatt ggctcgtcat ctgtttgttt cgatgattgt ttctcacctg
      541 tttgactgaa tgaagacaca atcggaaccg gcgtcaactg ccacccccaa ccagcacaaa
      601 aaaccacgat gaagagaaat accacctgac gtgtgaggtt tctctggttc aggcaacgaa
      661 aaggtggctt aaaactctga gttgggtcgt tactcataag gtttttcctg atgactggat
      721 cttgttttta aggagcgagc gttgctgttt cacccatcgg ttgcgataca ggtaatatct
      781 cggtctccag acatattcca gttcgacaaa tcttgattca gtttgccccg agacatttcg
      841 acaatagaac ccataacagc ttcgtgatgg tgaccaaacc cagatagcgt tgctaagcgg
      901 ggcatttcca tagccccaca atcctgaata cccgtcaatc aacgagtctc ctttgcggct
      961 ggtgtaaacc tgatcaaagt atgccccaag gatgcgcgcc cgctcgcgag tccatttgta
     1021 aaaaacatcc tccggtggtt tgctgatttc agattccagg agctggccag atttgaagac
     1081 aagttgcttc ggcgctccgt gccggcctgg cccagtgaca ccattttgga cttcaccttt
     1141 gtagaccaga caccgtccac gttgctcacc tgtggtttca aaccgatatc ggccttcaag
     1201 ctgaagtttc agttttgttt gggccagcac aatcgttggg ttgagtttga tttgttcata
     1261 ccctgggaac tcaatgattg aactcccggt ttccagttcc atttcaaact ggtcaaacct
     1321 cgtgtttttg acaatccagg ttgtcccggc atccagccaa atgaccgaac caaatccaag
     1381 caggacttcc agatagctat g
//
LOCUS       DAOQDO010002027         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111067_length_1401_cov_2.210996, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002027 DAOQDO010000000
VERSION     DAOQDO010002027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111067_length_1401_cov_2.210996"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKZ53_24685"
     CDS             <1..761
                     /locus_tag="PKZ53_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006790307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNJ43702.1"
                     /translation="YGIRSGDQDTRQIDVWIDCVNTSLSGLSLTVHWPQGTLEIQSPM
                     VGKPHAYNILASIATATACSIPVEHIVKGISQAQVPGRFERVDDGSQGFAVVVDYAHT
                     PDAIHNALGAARELVTQTGGRVITLFGCGGDRDRTKRPLMAEAAAQNSDILVATSDNP
                     RSESPEQILDDAEIGLRKVGKPFHRITDRRQAISFAIHQAQPGDIVVLAGKGHEPYQI
                     LADRTIHFDDREEARQALQNRLEPPHQEAACVHS"
     gene            748..>1401
                     /gene="murF"
                     /locus_tag="PKZ53_24690"
     CDS             748..>1401
                     /gene="murF"
                     /locus_tag="PKZ53_24690"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNJ43703.1"
                     /translation="MFIHEIGHLAGAKNLAELPAEVAEVTPVGFAIDSRSIEAGELFF
                     AIQGAVFDGHAFVKMAFERGACAAILSADVKDLTDAERQRCLMVENTLLALQQLAHRL
                     LTDWGRPVIGITGSAGKTTAKELTARTVSTCGRVHASVGNLNNTFGLPLTALKMISNH
                     HKMDDFDFAVFEMGMSFPGEITQLTQIAPPHISVVLNVAPVHLENFDSIEGIAAAKAE
                     "
BASE COUNT          327 a          381 c          387 g          306 t
ORIGIN      
        1 gctatggaat tcgctcaggt gatcaggata ctcgccagat agatgtctgg attgattgtg
       61 tgaacaccag cctttccggg ctgtcactca cggtccactg gccgcagggc acgcttgaaa
      121 ttcaatcgcc gatggttggt aaaccacacg cctacaatat tctggcgtca atcgcaaccg
      181 ctacggcctg ttcaattcca gttgaacata ttgtgaaagg aatttcacaa gcccaggttc
      241 cgggacggtt tgagcgggtt gatgacggaa gccaggggtt tgccgtggtg gtggattatg
      301 cgcacacccc agatgcaatc cacaatgcac tcggggcagc acgagaacta gtgacccaga
      361 ccggtggacg ggtgattaca ctctttggct gtggagggga tcgggatcgc accaaacgtc
      421 cgctgatggc tgaagcggct gcccaaaaca gcgacattct ggtggccacc tctgacaacc
      481 cgcgctctga gtctccggaa cagatcctcg atgacgccga gattggctta cgaaaagtgg
      541 gcaagccgtt tcaccgcatc actgaccgcc gccaggcaat ctcttttgcc attcatcagg
      601 cgcaaccagg tgacatcgtg gtgttagctg gtaaagggca tgaaccctat caaattcttg
      661 cagatcgaac gattcacttt gatgaccggg aagaagcccg gcaggcattg caaaatcggc
      721 tcgaaccacc ccaccaggag gctgcctgtg ttcattcatg agattggcca cctggcgggt
      781 gcaaaaaatc tggcggaact gccagccgaa gtcgctgaag tgacaccggt tgggtttgcg
      841 attgattcgc gcagtattga agcgggcgaa ctcttttttg ccattcaggg cgcggtattt
      901 gacggtcatg cctttgtgaa aatggctttt gagcgcggcg cctgcgccgc tattctttca
      961 gccgacgtca aagacctgac cgatgccgaa cgccagcggt gcctgatggt tgagaacacc
     1021 ctgctggcac tccaacaact ggcccaccgg ctgctgaccg actggggacg cccggtcatc
     1081 ggcatcaccg ggagtgccgg gaaaaccaca gccaaggaac tcaccgcccg cacggtttca
     1141 acctgtgggc gggttcacgc gtcggtggga aatctcaaca acacgtttgg cctcccactg
     1201 accgcactca agatgatttc aaatcaccat aaaatggatg atttcgactt tgctgtcttt
     1261 gagatgggca tgagctttcc tggagaaatc acccaattga ctcagattgc cccgcctcat
     1321 atcagtgtgg tacttaatgt ggcaccagtg catctggaaa actttgacag cattgaagga
     1381 attgccgcgg ccaaagctga a
//
LOCUS       DAOQDO010002028         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111081_length_1401_cov_2.014859, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002028 DAOQDO010000000
VERSION     DAOQDO010002028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111081_length_1401_cov_2.014859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PKZ53_24695"
     CDS             <1..983
                     /locus_tag="PKZ53_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43704.1"
                     /translation="EALREVLLNPTCPLVLSTHGTLPRSKNYTTFVYKIVRPWGEAAI
                     PLLQKKLVSPNPSVRSLAMSLLLEFSDFLFNIEKVERLIPSRDIGKALTREIDVAIFE
                     LRRTQFRSSPILIHFLANVRPFQNKFDEESPVFLQPTTYREWEFFEDLGDSWPQPDVV
                     CPQLAKPQERQFTKLDSRMVYSFYPDAYQSPSTKWSKAFEDRAFLKLLSQADRKAPTF
                     KQYFTKLRALRLYRLLSDPKPEIKVSAAISLVFQDDQIGQKVLETWLSSTSPLYKKLI
                     VNHFSRWISDKRKLVKFLPQFNQCATDPTLPAETHAQAKALVEKIKGDPR"
BASE COUNT          365 a          351 c          293 g          392 t
ORIGIN      
        1 atgaagcgct tcgagaggtt ttgctcaatc ccacctgccc cctggtactt tccacgcatg
       61 gaacattgcc tcggtcaaag aattacacca catttgtcta caaaatcgtt cgaccctggg
      121 gagaagctgc gattccgctg ctgcaaaaga aattggtgtc accgaaccct tccgtccgat
      181 ccctggccat gagtcttctt ttggaattca gtgattttct cttcaacata gaaaaagtag
      241 agagactaat tccctcacgc gacatcggaa aagcactgac ccgggaaatt gacgtcgcta
      301 tttttgagct tcggagaacg cagttccgat caagtccgat tctcattcac ttccttgcta
      361 atgtgcgtcc ttttcaaaac aagtttgacg aggaatcccc agtttttctc caacctacaa
      421 cctatcggga atgggagttt ttcgaagacc ttggcgatag ctggccgcaa ccagacgtgg
      481 tttgtccaca attggccaaa ccacaagaga gacaatttac caaactggac tccagaatgg
      541 tctattcttt ttaccctgat gcataccaat caccctcaac gaagtggagc aaagcttttg
      601 aggatcgggc atttctcaag ctcctgtctc aagcagatag gaaagctcca acctttaaac
      661 aatacttcac gaaactccgt gccctccggt tgtatcggct tctttctgat ccaaaaccgg
      721 aaatcaaagt cagtgccgcc atcagtctgg tcttccagga tgatcagatc gggcaaaaag
      781 ttctcgaaac ctggttgagc agcaccagtc cactctataa gaaactgatt gtgaaccact
      841 tcagcaggtg gatttccgac aagaggaaac tggtcaagtt tctcccccag ttcaatcagt
      901 gtgccaccga tccaacgctt ccggctgaaa cccatgctca ggccaaagcg ctggtggaaa
      961 agataaaagg tgatcctcgt tgaaatcctc gaagccatca cgcggtggat gtgattttgc
     1021 cacaggcaac cttgaattct ttttggcttt gacaatgccg ggtcaagagg caggtttctc
     1081 ttatgctctc attggatttt ggcgaattgc aattttccaa agtatactta accagttgat
     1141 tgaagactgc ctcaggattt cctcctcaat tttcaaaaga acgaaactcc aggaagaacg
     1201 agaaggtgag ctgattgtaa ctgttcaccc ttacgatatg attatgtttg attttcgtca
     1261 ccacagagac acaaaggaca cggagtttga aaacaagttt tcaagatttt gcttttctct
     1321 tcttcgtatt tctctgtgct ccccgtgtct ccgtggttaa atatgtcttc cgttcctttt
     1381 ctgtgttatt ctgtgttttc t
//
LOCUS       DAOQDO010002029         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111163_length_1400_cov_3.163569, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002029 DAOQDO010000000
VERSION     DAOQDO010002029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111163_length_1400_cov_3.163569"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKZ53_24700"
     CDS             complement(<1..877)
                     /locus_tag="PKZ53_24700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43705.1"
                     /translation="MNLTAALKKLLSELLFPIIAVIAAFVIGGVIVLCIGDSPLLVYE
                     LFFSSAFGTLDGISYTLFYATPLIFTGLAVALAFHCGLLNIGCEGQLSVAAFATAWVG
                     FTLPVYLGEAVSPTVKSLILIPACILGGIVMAGLWGAVPGFLKAKFGSHEVINTIMLN
                     FIAAAIVSYLTQYHFRTVGDQILETFEIDKAAHIPRFHHLLAPLGITYPERLPLNIGF
                     IIALLTCVAVYVFLWKTKWGYELRAVGNNASAAEYGGISVKRNIILAMTISGGLAGMV
                     AISEVQWLTGFRIDLA"
     gene            complement(999..>1400)
                     /locus_tag="PKZ53_24705"
     CDS             complement(999..>1400)
                     /locus_tag="PKZ53_24705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43706.1"
                     /translation="VIDYLNEHKRHFIVRSDLKHIIGEIVDNPLPQMIYFWEGLSDDE
                     KLVLSLLAEVISDDSQFATAARLARVIVRNDYPVTLSENTIRLTLEELFRSEILIKEG
                     NEDFAYRIDLMRYWIKRSHSIWQVVREVKTL"
BASE COUNT          386 a          340 c          362 g          312 t
ORIGIN      
        1 cggcgaggtc gatgcggaag cccgtcagcc actgcacttc gctgatggcc accattcccg
       61 ccaatccacc tgagattgtc atggccagaa taatgttgcg tttgacactg atgccaccat
      121 attcggctgc cgaagcattg ttgccaactg cccgtaactc atatccccat ttggttttcc
      181 acaaaaatac atagacggcg acacaggtaa gaagggcaat gatgaaccca atattgagcg
      241 gcaggcgctc tggataagta attcccagcg gggccagcaa atggtgaaac cggggaatat
      301 gggcggcttt gtcaatttca aaagtttcaa gaatctgatc cccaacggtt cggaagtgat
      361 attgcgtcag atagctcaca atggcggcag caatgaagtt caacataatg gtgttgatca
      421 cttcgtgcga gccaaacttc gctttcaaaa agcctggaac cgcaccccag agtcccgcca
      481 tcacaattcc gcccaggata caggctggta tcaagatcaa tgatttaacc gttgggctaa
      541 ccgcctcgcc aagatagacc gggagcgtaa acccaaccca ggcggtggca aaggcagcca
      601 cactgagctg gccttcacac ccgatgttga gcaacccaca gtgaaatgcc agtgcgaccg
      661 ctaacccggt gaaaatcaac ggggtggcat aaaataacgt gtagctgata ccgtcgagtg
      721 ttccaaaggc actgctgaaa aagagttcat acaccagcag cgggctatcg ccgatacaca
      781 gcacgatcac accgccaatc acaaaggctg caatcacggc aatgatcgga aataagagtt
      841 cagaaagcag tttttttagg gcggcagtga gattcatggg caatgagtgg ttggcagttg
      901 gtggttggcg gttggtggtt ggtggttggt ggttggtact tagaaccaat cttttcgaag
      961 aacgaagaac gaagcgccag gcaccaggca cgaagtactt agagagtctt tacctcgcga
     1021 acgacttgcc agatgctgtg gctgcgtttg atccagtacc gcatcagatc aatgcgataa
     1081 gcaaaatcct cgttgccttc cttgatgagg atttcactgc ggaataattc ttcgagcgtg
     1141 aggcggatgg tattttctga aagcgtgacc ggatagtcat ttcggacaat gactcgggca
     1201 agcctggcag ccgtggcaaa ttgagaatca tctgaaatta cttcagccag cagggaaagc
     1261 acaagctttt catcgtcact gaggccttcc cagaagtaaa tcatttgcgg aagcgggtta
     1321 tcaacaattt ccccaatgat atgtttgaga tcagagcgca caataaagtg gcgtttgtgc
     1381 tcatttaagt agtcaatcac
//
LOCUS       DAOQDO010002030         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111172_length_1400_cov_2.826766, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002030 DAOQDO010000000
VERSION     DAOQDO010002030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111172_length_1400_cov_2.826766"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKZ53_24710"
     CDS             complement(<1..687)
                     /locus_tag="PKZ53_24710"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ43707.1"
                     /translation="MMRIKTLLCLVLAVLPLVGMWKLEIAGKPQARGQRVPVKTETGT
                     EVLLYAESHALVIGVSEYQRGTGWGTLPGVKDDVPAVRSALEQHGFQVEVVLNPTGSQ
                     LRQALNQFAEDWGFAEGNRLVIYFAGHGYTGKTIDGREMGYIVPQDAPDPDQDNARFN
                     KVAVSMKEIMNIAERVQAKHALFLFDSCFSGTLFKTRERVSPSIVQKTARPVREFITA
                     GTARQAVPDQS"
     gene            1144..1365
                     /locus_tag="PKZ53_24715"
     CDS             1144..1365
                     /locus_tag="PKZ53_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43708.1"
                     /translation="MDDSTKKKAIAAAKITFGAARVISGIATATGHGLLGTYLKSHHM
                     RSQAVALGKRSVDGGIAMFKNGLDDWKKS"
BASE COUNT          330 a          389 c          332 g          349 t
ORIGIN      
        1 gctctggtcc ggcactgcct gtctcgccgt tcctgccgtg ataaattccc gcaccgggcg
       61 ggccgttttc tgcacaatcg aaggagagac ccgttcacgg gttttgaaca acgtcccgga
      121 aaaacaactg tcaaacagaa acagggcgtg tttggcctgc acccgttcgg caatgttcat
      181 gatttctttc atgctcacgg caactttgtt aaatctagca ttgtcctggt ccgggtcggg
      241 cgcgtcttgc gggacaatgt agcccatttc acggccatca atcgtttttc cggtgtatcc
      301 gtgccccgcg aagtagatca ccagtcggtt cccctcggca aagccccaat cttcagcaaa
      361 ctggttgagc gcctggcgca actggctgcc ggttggattg agcaccacct caacctgaaa
      421 gccgtgttgt tccaatgccg accggactgc cggcacatca tctttgactc cggggagtgt
      481 tccccatccg gttccccgtt gatattcgct gaccccgatc accagggcgt ggctttcggc
      541 gtacagcagg acctcggtgc cggtttcggt tttgaccggt acccgttgcc cgcgagcttg
      601 cggtttcccc gcaatttcca gtttccacat tccaaccagc ggcaggaccg ccagcaccag
      661 acacagtaac gtttttatgc gcatcatatt ttccctcacc tgtcatctat gagttgtgcg
      721 cccggttata ccataccgac ctggagttta gtgtatctga tttggaaaaa tgtgttgtga
      781 gccgcaggaa attggcggtt ctgccctggc tgactgacgc ccgggcaatg catctctcgt
      841 tctgccgtca aactaaatta ttgaaaaaac tgtatttctc tgacccctga ccccgagccc
      901 ctgatcccaa aatgatatta ttttcctaat ctgatacggt gtgatttgga aaatgcccct
      961 agagtagcac tatcgaacct gctcggcagg aataaccatc actcgaaaac aaattcaaat
     1021 ttagattcag ggaatcagag ccatctgaca cgaaaagaca aatcccggaa gggtttactc
     1081 ttttccgaat caagggcact agctaccaaa tcttgttcca tcactgatca acaggaggtt
     1141 ttcatggacg attccaccaa gaaaaaagca attgccgccg caaaaattac ttttggagcg
     1201 gcgcgtgtca ttagcggtat cgcaacggcc acaggtcacg gactccttgg tacctacctg
     1261 aagtctcacc acatgcgaag tcaggcagtc gcgcttggaa aaagaagcgt agacggtgga
     1321 attgcgatgt ttaaaaacgg cctcgacgac tggaaaaagt cttaacctaa atcattgatt
     1381 ttgctcattg cctgacaatt
//
LOCUS       DAOQDO010002031         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111338_length_1399_cov_3.213542, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002031 DAOQDO010000000
VERSION     DAOQDO010002031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111338_length_1399_cov_3.213542"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..710)
                     /locus_tag="PKZ53_24720"
     CDS             complement(87..710)
                     /locus_tag="PKZ53_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43709.1"
                     /translation="MNSKPLLSAYLAALPVQDRVAARSQSGPGPLQAWLWGLSAWGQL
                     VQTRAGYALALEAFSQWKTFLTQSPDQNWPEAVCLENCWKQFQVWIDTRDHTSFTELK
                     TLQPELAQFLNIVDLNIAEGVRPEEPYWAALVCSHAISVALWDTAQLPSGLSQPEYDQ
                     RLRAGPAFDLLEAAFCAKKAFGWTDEQIRACIAQKLSEESPGDLESQ"
     gene            complement(823..1173)
                     /locus_tag="PKZ53_24725"
     CDS             complement(823..1173)
                     /locus_tag="PKZ53_24725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43710.1"
                     /translation="MGIDFSHGDARFGYGGFNYFRQNLAKAIGIDLLAMQGHGGSQSW
                     EGISDPLVLFLQPFDVEIKIPPDLCPAVADRLLEVTTTFTPPDGWKNADAVALEVIEG
                     LREAATRGELFKSF"
BASE COUNT          347 a          353 c          339 g          360 t
ORIGIN      
        1 cactctatga ctgttgtggc tttgatttca atcaaaaatt caggcctgac gagggttgaa
       61 atgcttattc agaatccagt gacgtgtcat tgactttcga ggtctccagg actttcttca
      121 ctgagctttt gcgcgataca cgcccgaatt tgctcgtcag tccagccaaa cgcttttttg
      181 gcacaaaagg cagcctccaa gaggtcaaat gcgggtccag ccctcaagcg ctggtcatat
      241 tcaggttgtg aaagcccaga tgggagctga gcggtgtccc acaatgcaac cgaaatcgcg
      301 tgtgagcaca ccagcgctgc ccaatatggc tcttctggtc gaactccttc agctatattg
      361 agatccacga tgttcagaaa ctgtgcgagt tccggctgaa gtgttttgag ttccgtgaaa
      421 gaagtgtgat cgcgggtgtc tatccacact tgaaactgct tccagcagtt ttccaggcac
      481 accgcctcgg gccagttctg gtctggcgat tgagtcaaga aggttttcca ttgcgaaaaa
      541 gcctcaagtg ccagcgcata tccggctctg gtttgtacca actgtcccca ggccgaaagc
      601 ccccaaagcc acgcctggag cggacctgga ccggattgag agcgggcggc gactcgatct
      661 tgtactggaa gggcagccag ataggccgac aataatggtt tggaattcat ttttcccatc
      721 gccttttgaa atgatccagt caggcgaaat aactgaaaga tctgtgtttc cctcaatctc
      781 aagttccatt gcctcatccc cggataaaac tctccgggag agttaaaacg atttaaatag
      841 ctctcctctg gtcgctgctt cgcgaagtcc ctcgatgacc tccaaagcca cggcatcagc
      901 gtttttccat ccgtcaggcg gtgtgaaagt ggtcgtgact tccaggagac ggtctgccac
      961 tgcaggacaa agatcgggtg gaatctttat ctcaacatca aatggttgaa ggaacagaac
     1021 cagcggatca gaaatccctt cccacgactg tgatccaccg tgcccctgca tcgccaggag
     1081 atcaattccg atagctttcg ccagattctg gcggaaatag ttaaaccctc cgtatccaaa
     1141 acgcgcgtcg ccatgtgaaa aatcaattcc catttttcgt cctttcaagt tgttattctt
     1201 ttacaacggc aatcagtgat tgttgatgaa agcttctcag caggtttgtt ttgtgtaagt
     1261 tgaggcatcg cttgactgaa gattgaatcg gcgatgaatg cctcaaagta caaaggagta
     1321 ctggctggaa ccagcaacga tgaggattat accagtttgt agtcagtaga tcgtagtcag
     1381 tagttcacta accctattt
//
LOCUS       DAOQDO010002032         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111554_length_1398_cov_1.982130, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002032 DAOQDO010000000
VERSION     DAOQDO010002032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111554_length_1398_cov_1.982130"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1375
                     /gene="truB"
                     /locus_tag="PKZ53_24730"
     CDS             428..1375
                     /gene="truB"
                     /locus_tag="PKZ53_24730"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNJ43711.1"
                     /translation="MIPTLLNGAVLLDKPMGITSHDVVARLRYVLRTKRIGHTGTLDP
                     FATGLLILCVGSSTRLSQFLTGKDKTYHATMRLGFATDTQDYTGKPLSEPNSTEVVTE
                     DNLREAMRHYTGDYAQVPPMYSAKKVDGISLHRLARQGQVVFRSTAPVKIYELTLNPD
                     EQGNTIRRNETGTVDVDFTVSCSAGTYIRTLAHDIGQHLGCGAHLTALRRTRVGPFAI
                     EQATTIEQLAGLPMEQIRASLLKPVELLKDWKLFQVSVTDHLKFVQGQAIPLDISGLD
                     PISEESDLLVGICTESNDLLGIARLNSQGKARPQVVFNE"
BASE COUNT          334 a          358 c          325 g          381 t
ORIGIN      
        1 gtattgattt gacgcggggg cctgtacgtt gtatccaaag ctcacatcac tctagcccag
       61 agcgcttcaa ctttcaacca aaagcaaatt cactttctcg ctcttgattc aggatttttc
      121 agcattttgg gcttattttt gatttggaat atctggcagt gaaactgact ggcgtttacg
      181 gtgttgtttt tcaacttgga tgttgaagaa ttattttttt ctgggcttta tcaaaatgga
      241 cctcgtgttt gatgcacgct ggtcaaatcc tgtgatacaa atttttattt ccacaaataa
      301 aaaattcctg cttttttcag tttcagaaca cactctgggg ttcaactcgc tcgtggaacc
      361 gtctacaatt tgccgatttc attcagcgtc actatcaatt cactcatttc tccttctacg
      421 gactcccatg attcctacat tgttgaatgg tgctgttttg cttgataagc caatgggcat
      481 tacgtcccat gatgtcgtgg cccgtctccg ctatgtgttg cggaccaaac gcatcggaca
      541 caccggaaca ttagacccct ttgcaaccgg gcttttgatt ctgtgtgttg gttcaagtac
      601 tcgattgtcc caattcctga ctggcaaaga caaaacctat cacgccacca tgcggctggg
      661 atttgccact gatacccagg attacacggg taaaccactc tctgagccca actcgaccga
      721 ggttgtcact gaagataacc tgcgcgaagc catgcggcat tataccggcg actatgccca
      781 ggtaccgccg atgtattcag ccaaaaaagt ggacgggatc tcgttgcatc ggctcgcccg
      841 ccaggggcag gtcgtgtttc gctcgaccgc accggtcaag atttatgaac tcacgttgaa
      901 cccagatgag cagggcaata ccatccgacg caatgaaact ggtacggttg atgttgattt
      961 tacggtttcc tgctcggcag gcacttatat tcggaccctg gctcatgaca ttggccagca
     1021 tcttgggtgt ggagcccacc tgacggcact ccgtcgaacg cgggttggcc catttgcgat
     1081 tgagcaggca accacgattg aacaactggc cgggctcccg atggagcaaa tccgggccag
     1141 cttattaaaa ccagtcgaac tcctcaaaga ctggaaattg ttccaggtat cagtcaccga
     1201 ccatctgaaa tttgtgcagg gacaggccat cccgctcgat atttctggac tggatccaat
     1261 ttcggaggaa tcagacctgc tggtggggat ttgcaccgaa agcaatgacc tgctcggaat
     1321 tgcccggctt aactctcaag ggaaagcccg accgcaggtt gtttttaatg aataagttat
     1381 gagcacccca cacttaaa
//
LOCUS       DAOQDO010002033         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111655_length_1397_cov_3.067809, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002033 DAOQDO010000000
VERSION     DAOQDO010002033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111655_length_1397_cov_3.067809"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1397
                     /locus_tag="PKZ53_24735"
     CDS             <1..>1397
                     /locus_tag="PKZ53_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ43712.1"
                     /translation="RLGGGPKKIEQQHSQGKLTARERVTRLLDPNTYFQEIGLLMAYD
                     QYDGQAPGVGVITGIGWCCGREVVVVANDATVKAGAWWPETITKMLRAQEIAMRSRVP
                     IIYLVDSAGVNLPYQGGVFPGQYGAARIFHYNSIMRRHLKVPQLAAVMGQCVAGGAYL
                     PALSDVIVMVEGTSFMGLGGPNLVKGATGQVVDAETLGGARMHNEISGVAHYREPNDE
                     ACLERLRKLVLELPPRPASDVTLVESRSPKRAEADLYQIIPNDHKLPYDIREVLDCLV
                     DADSFEEFQADFAKEVICAHVRLRGLPVGLITNQRGLIKGAKGKPPRFGGIIYRESAE
                     KIAYFIETCNRHRTPLLFVQDVSGFMVGTDAEQSGIIRAGAHFVEAMATATVPRLVLT
                     VNHASGAGYYAMAGQGFDPDFIFSWPTGRMGVMEGDSAVMALFSAQLDKIKKEGKEPD
                     EALKAEMEKVRATYE"
BASE COUNT          317 a          339 c          413 g          328 t
ORIGIN      
        1 tcgactcggc ggaggtccca aaaaaatcga acaacaacac agccagggca aacttacagc
       61 ccgcgaacgg gtaaccagac tgcttgaccc gaatacctac tttcaggaaa tcgggttgtt
      121 gatggcctat gatcagtatg atgggcaggc accaggtgtc ggtgtcatca ccgggattgg
      181 ctggtgttgt ggccgcgaag tggtcgtggt tgccaatgat gccacggtca aggctggcgc
      241 ctggtggccg gaaacaatta ccaagatgct ccgggcgcag gaaattgcca tgcgctcacg
      301 ggtaccaatt atttatctgg ttgattcggc tggggtaaac ctaccgtatc agggtggggt
      361 tttccctggt cagtatggtg cggcacgcat tttccactat aactcaatta tgcggcgcca
      421 tttgaaagtt ccccaactgg ctgcggtgat ggggcagtgt gtcgctggtg gggcctattt
      481 gccggccctt tccgacgtga ttgtgatggt tgaaggcact agctttatgg ggcttggcgg
      541 cccgaatctg gtcaagggcg cgaccgggca ggtggtggat gccgaaaccc ttggcggagc
      601 acgcatgcat aatgaaattt caggtgtggc tcattatcgc gagccaaacg acgaagcctg
      661 cctcgaacgc ctgcgcaagc tggttctgga attgccaccc cggcccgctt cagatgtcac
      721 actggttgaa tcacggtcac caaaacgcgc tgaagccgat ctctatcaaa ttattccaaa
      781 tgaccataag ctgccttatg acattcgtga agtgcttgat tgcctggtgg atgccgatag
      841 ttttgaggaa tttcaggctg attttgccaa agaggtgatt tgtgcccacg ttcgactgcg
      901 agggctgccg gttgggttaa tcaccaacca gcgcgggttg attaaaggtg ccaaaggcaa
      961 accacctcgc tttggcggca ttatctatcg cgaaagtgct gaaaaaatcg cctatttcat
     1021 tgaaacctgc aatcgccatc ggaccccttt gttatttgtc caggatgtat caggatttat
     1081 ggtcggaacg gatgccgagc agtcagggat tatccgggct ggcgcgcatt ttgtcgaagc
     1141 catggccacc gcaactgttc cacggctggt actcacggtc aaccatgctt ccggcgccgg
     1201 gtattacgcc atggcgggcc agggatttga tccggatttt attttctcgt ggccaactgg
     1261 tcgaatgggg gtgatggagg gagattctgc tgtgatggcg ctcttttcag ctcaactgga
     1321 caaaatcaag aaggaaggga aggaacccga cgaggcattg aaagccgaaa tggagaaagt
     1381 acgggccacg tacgaag
//
LOCUS       DAOQDO010002034         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111729_length_1397_cov_1.997019, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002034 DAOQDO010000000
VERSION     DAOQDO010002034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111729_length_1397_cov_1.997019"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..279
                     /locus_tag="PKZ53_24740"
                     /pseudo
     CDS             23..279
                     /locus_tag="PKZ53_24740"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
     gene            complement(840..>1397)
                     /locus_tag="PKZ53_24745"
     CDS             complement(840..>1397)
                     /locus_tag="PKZ53_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNJ43713.1"
                     /translation="DEFGNAGSVSFLQDIMHPISVARMVMEKTPHVMLSGKGAQMFAV
                     DNGFPLQNLLTEKARKAWEDWKVKSEYKPVINIERHDTIGIVAMDAAKRISGACTTSG
                     LAFKMHGRVGDSPVIGAGMFSDWPVVGAVAASGAAPIFFSPVLGNLVDGGVGVHGNPC
                     LAATAEAMEYIGAAEGWYSGHFAGR"
BASE COUNT          320 a          363 c          366 g          348 t
ORIGIN      
        1 tgcctataac cggatttatg ccctgtgcga agatcgggca gggacactgt gggttggaac
       61 tatcggcggg ggcttgagtt actacaaaaa cggcacgttt ggcaccgtgc ccaattttcc
      121 atcgaaaatt gtcgaagcca tttttgagga tcacctgggc aatctgtggt ttggcactga
      181 cggttctgga ctggtgcgct ttaaagatgg aatgtatacc cggtttgaaa gcgcggttgg
      241 aatcctcaat caatcggttc attcgtttta tgaggacgcg gctggaacgt tgtggatgtg
      301 taccaacgga gggatcagtt gttaccaaaa cggcgtcttt tacaatttca cccgggatga
      361 tggcattttt gaaaatcaat tgttccagat tctggaagat gggcacggga gtttctggtt
      421 gagctgcaat cggggcattt tttcggtcaa acgtgaagaa ctcgaagctt ttatggccgg
      481 aaaaatcctc aaaatcaaat ccacggtgta tggaatttcc gacgggatga agagcaatca
      541 atgcaatggc ggttcacaac cagccgggtg tcggacacat gatggacgcc tctggtttgc
      601 caccattgga gggttgacgg tgattgaccc gcaaaacatc cggaccaatt tacttccgcc
      661 gcctgtcgcc atcgaagccc tcgacgtgga tggaacaccg gttgatttga gtcaggcggt
      721 tgaattgaag ccagggtcag accggattga gttttcctac gccgggctga gttttctgtc
      781 acctggcaag gtcaagttta aagtcttttt ggaaggctat gaccacgatt gggttgaagt
      841 cagcgcccgg cgaaatgtcc gctataccaa ccttccgccg cgccgatata ttccatcgcc
      901 tcagccgttg ccgccaagca cgggttgcca tgcactccca ccccgccatc gactaaattg
      961 cccaacacag gtgagaagaa aatcggcgct gcgccgctgg ccgccaccgc gcccaccacc
     1021 ggccaatcgg agaacatccc tgcgccgata acgggactgt cgcctaccct gccgtgcatt
     1081 ttgaatgcca atccactggt agtgcaagcg ccgctgattc tctttgcagc gtccatagcc
     1141 acaattccaa tagtatcgtg gcgttcgata ttgatcacag gcttgtattc cgatttgacc
     1201 ttccagtctt cccatgcttt tctggccttt tccgtcagga gattttgcag gggaaaaccg
     1261 ttgtcgaccg caaacatctg ggcgcctttt ccgctcagca tgacgtgcgg cgttttttcc
     1321 atcaccatgc gcgctacact aatcgggtgc ataatatcct gcaaaaaaga aaccgaaccg
     1381 gcattgccga attcgtc
//
LOCUS       DAOQDO010002035         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111800_length_1396_cov_3.350485, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002035 DAOQDO010000000
VERSION     DAOQDO010002035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111800_length_1396_cov_3.350485"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1319
                     /locus_tag="PKZ53_24750"
     CDS             126..1319
                     /locus_tag="PKZ53_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ43714.1"
                     /translation="MYRLSIRQLLLIIIGTAFLTGGTVFGLYYFLGKYLPHEKEVNGQ
                     SGQPPRSGTPLVLGSDEKNNIDIYEKFGPGVVNITSTAIVEDFFWGPYRQSGGGSGSI
                     IDDKGNILTNFHVINNAQELIVTLADKTTHVAKVVGADPNNDLAIIKIDVPKEKLHIV
                     PLGTSQNLKVGQKVLAIGNPFGFERTLTIGVISGLGRPLRTESGRTIDNVIQTDASIN
                     PGNSGGPLLNSAGEIIGINTAIYSPSKGSIGIGFAVPVDVARQVIPDLLTFGRVRRPW
                     LGIETFAITPRLVRRYSLPVNEGLIITKVVPAGPSDRVGIRGSNQVQRRPDGRETVLG
                     DILVKVGDQDVKTEEDLYRALKDRKIGETVQVVVYREGQPTRLAVKLDELPARYESFR
                     PDPEE"
BASE COUNT          353 a          327 c          379 g          337 t
ORIGIN      
        1 catccttcat ccctcatccc ttcaattgcc ccgagcttgc gagtcttcag ccccaggccc
       61 gatgttttca gcccaaagtc ttcagcccca aatgaaagaa aaacaactat tgaggaactg
      121 tgaatatgta ccgattaagc attcgacaac tcttgctcat cattataggc acggcattcc
      181 tgaccggggg caccgtgttt ggtctgtatt attttctggg aaaatacctt ccccatgaaa
      241 aagaggtaaa tgggcaaagc ggccagccgc ctcgttctgg cacaccgctc gttttgggaa
      301 gcgacgaaaa aaataacatt gatatctatg aaaaatttgg tcccggtgtg gtcaacatca
      361 ccagtacggc cattgtggag gatttctttt gggggccata tcgtcaatct ggtggtggtt
      421 caggctcgat cattgatgat aaaggcaaca ttttgaccaa tttccatgtg atcaataatg
      481 cccaggagct gattgtgacc ctggctgaca aaacaaccca cgttgccaaa gtggtcggtg
      541 ctgacccaaa caacgatctg gcaatcatta aaattgatgt gcctaaagaa aaactccaca
      601 ttgtgccgct cggcacctct caaaacctga aagttggaca aaaggtgctc gcgattggaa
      661 acccgtttgg gtttgaacga accctgacca ttggggtgat cagtgggtta gggaggccac
      721 tccggactga aagtggacgc accattgata acgtcatcca gacggatgct tcgatcaatc
      781 caggaaattc cggtggaccg ctgttgaatt ccgccgggga aatcatcggg atcaacaccg
      841 ccatttacag cccgagcaaa ggaagtatcg gcattggttt tgctgttcca gtggatgttg
      901 cccggcaggt gatccctgat ttgctgacct ttggccgtgt ccgcaggccc tggcttggaa
      961 ttgaaacctt tgccattacg ccgcgtctgg tccggcgata tagtttgccg gtaaacgaag
     1021 ggctcattat caccaaagtc gtgccggctg gtccttcaga tcgagttgga attcggggca
     1081 gcaatcaggt tcagcggcgc ccggatggac gtgaaacggt gttgggagat attttggtga
     1141 aagtcggaga ccaggatgtc aaaaccgagg aagatctcta tcgggcgctc aaagaccgga
     1201 aaattggcga gacggttcag gttgtggttt accgtgaagg ccagcctacc cgcttagctg
     1261 tcaaactgga tgagttgccc gcccggtatg aatctttccg tcctgaccca gaagaataaa
     1321 atcatttggg gttgagggtt gagggttgag ggttgagggt tgagggttga gggttcaggg
     1381 taaagacgaa aaggac
//
LOCUS       DAOQDO010002036         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111829_length_1396_cov_2.745712, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002036 DAOQDO010000000
VERSION     DAOQDO010002036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111829_length_1396_cov_2.745712"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..>1396
                     /locus_tag="PKZ53_24755"
     CDS             179..>1396
                     /locus_tag="PKZ53_24755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43715.1"
                     /translation="MLRYFSEIRLAFSNLQSHKLRTFLTMLGMIFGVGAVIAMLSIGA
                     GAEQESLRLIDTMGVRNIIIRDREFKDEDLKKNRENSLGLSLRDAQNIVAVAPDIELH
                     ACKKRVKTFQIFSYQGKADSANVIGATPAYFRLVKYQLSEGNFLAEPDEREFEQYCVI
                     GSRVKQKLFSSMSPIGQLIKINNLWFRVIGVLADNTLTKDEFEGVKLQDFSQDIYIPL
                     STALKKFELKKLESELDEIIVSVKTTDAIKPSAVLISQILTSTHGQVEDFSLIVPREL
                     LEQNQRTQRIFNIVMSCIASISLLVGGIGIMNIMLANILERTREIGVRRALGARQRDI
                     WMQFLIEALTISLLGGAIGVVFGFGVSRVVALYAEWSTVVTTTSIGLSFGVSAAVGLI
                     FGIYPAVRASRLDP"
BASE COUNT          346 a          321 c          351 g          378 t
ORIGIN      
        1 cgtcgccaaa acttaagaat taaattggat ccacaagttt tccacacggg atccacaggt
       61 tttccacaga ttttgacaag ttttccacag atttcggtaa gttttccaca ggcactcctg
      121 aaaataatgt ggagtgcggt ggcttgacac cgctttcgac ctgaccccca gaacccctat
      181 gttgcgctat ttcagtgaaa tccgactggc cttcagtaat ttgcaaagcc ataagctgcg
      241 caccttttta accatgctgg ggatgatctt tggcgtgggt gccgtcattg ccatgctttc
      301 gattggtgct ggggccgaac aggaaagcct gcggctgatt gataccatgg gtgtgcgtaa
      361 catcattatc cgcgaccggg agtttaaaga cgaagacttg aaaaagaatc gtgagaattc
      421 acttggtttg tcgctccggg atgcccaaaa cattgtggcc gtggcccccg atattgaact
      481 gcatgcctgt aaaaaacgcg tgaagacgtt tcaaattttt tcttatcaag gcaaagccga
      541 tagtgccaat gtgattggtg ccacgccagc ttactttcga ctggtcaagt atcaattatc
      601 agagggcaat ttcctggcag agccggatga acgcgaattt gaacagtatt gcgtgattgg
      661 ttcacgagtg aaacaaaagc tcttcagttc gatgtctcca attggccaat tgattaaaat
      721 caataactta tggtttcgtg taattggtgt tctggccgat aacacgctga caaaagacga
      781 gtttgaaggt gtcaaacttc aggatttcag ccaggatatc tatattccgc tctcaaccgc
      841 actcaagaaa tttgaactca agaagctcga atcagaactc gatgaaatca ttgtttcggt
      901 gaaaacaacc gatgccatca aacccagtgc cgtgctcatc agtcagattt tgaccagtac
      961 ccacggtcag gttgaagatt tttcattgat tgtcccacgc gagttactgg agcaaaacca
     1021 gcgaacccag cgaattttca acattgtgat gagttgtatt gccagcatct cgcttttggt
     1081 gggtgggata ggcattatga acattatgct ggccaatatc ctggaacgaa cccgcgaaat
     1141 cggagtccgt cgggcacttg gggcccgtca gcgtgatatc tggatgcagt ttttgatcga
     1201 agccctgacg atcagcctcc ttggcggagc gattggggtt gtgtttggat ttggtgtatc
     1261 gcgggtggtc gcactctatg ctgaatggag cacggtcgtg acgaccacct caattgggct
     1321 ttcgtttggc gtgtcggcag ccgttggatt gatcttcggg atttatccag ccgttcgggc
     1381 ttcacgactc gacccg
//
LOCUS       DAOQDO010002037         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111832_length_1396_cov_2.695749, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002037 DAOQDO010000000
VERSION     DAOQDO010002037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111832_length_1396_cov_2.695749"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1289
                     /locus_tag="PKZ53_24760"
     CDS             177..1289
                     /locus_tag="PKZ53_24760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ43716.1"
                     /translation="MKRFVRQNRVMFINSISMGLPSVRSRDFFAKVRRKLKSYLKFVR
                     RTPEGIWVVTPIVLPFFSSALGRNINLALLQFQIRFLAWILGFRTPVLWVAIPTARDM
                     VGRLNESALIYQVSDKYDANTMDHGDRSALIRKLHADLIEAADVVYYSGRKLLADATV
                     GLEKSRLLEQAVDFEHFASTTSKTWDCPPELKEIKAKGQPILGYFGAIETWLIDQDLI
                     RYVTERHPDWQFVFIGLKTSPLDIESLPNVHCISSQPYANLPAFAGQFDVCVLPWVTN
                     NEFVNYGSAIKVREYLATGKPVVITPLYEYEPLDGILRIVRSYDHFIECIESVLATDS
                     LESRQSRQQAVMGSTWDVRAEQVSEVIETILKHKKV"
BASE COUNT          347 a          353 c          355 g          341 t
ORIGIN      
        1 aagaaccaag aaccaagcac caagaaccaa gaaccaagaa ccaagaacca agcacgaaga
       61 actaagcacc aaaaaccaag caccaagaac ccgacattgt gattcaaaat caatccatca
      121 tttgttttgc cggcgaggac tggtggtatc accatccaca ttcaaaaaat cacctcatga
      181 agcggtttgt ccgtcaaaac cgcgtgatgt ttatcaactc gatttcaatg gggctcccca
      241 gtgtcagaag tcgagacttt tttgccaaag tccgccgcaa gctcaaaagt tacctgaaat
      301 ttgtgcgccg gaccccggaa ggaatctggg ttgtaacccc gattgtcctt cctttttttt
      361 caagtgcgct tggccggaac atcaacctcg ccttgctcca gtttcaaatt cgatttctgg
      421 cctggatttt gggttttcgg acgccggtgc tctgggtggc gattccaacc gctcgcgaca
      481 tggtcggacg gttaaacgaa tcggccctga tttatcaggt ttcagataaa tatgacgcca
      541 acacgatgga tcatggcgac cggtcagcac tgattcgcaa gcttcatgct gatttgatcg
      601 aagcggcgga tgttgtctat tactctggcc gaaagttgct ggctgatgcc acggttggac
      661 tggaaaaatc gcgcttgctt gagcaggcgg ttgatttcga gcattttgct tcgacgacat
      721 cgaaaacctg ggactgccca cctgaactga aagaaatcaa agccaaaggg cagccgattt
      781 tgggctattt cggagcgatt gaaacctggt tgattgacca ggatttgatc cggtatgtca
      841 ccgaacggca tccggactgg cagtttgtgt ttatcggctt aaaaaccagc ccgttggaca
      901 tcgagtcttt gccgaatgtc cactgcattt cgtcccaacc ctatgccaac ctgccagctt
      961 ttgccgggca gtttgatgtg tgtgtcttgc cctgggtgac caacaacgaa ttcgtcaact
     1021 acgggagcgc gatcaaagtc cgcgaatacc tcgccaccgg caaacccgtt gtcatcacgc
     1081 cactctatga atatgagccg ctggacggca ttctgcggat tgttcggagt tacgatcact
     1141 tcatcgaatg tatcgaatca gttctggcaa cggattcgct tgagtcgcgc cagtcccggc
     1201 aacaggctgt catgggcagc acctgggatg tccgggcgga acaggtgagc gaagtgattg
     1261 aaaccatcct taagcacaag aaagtttaga gttttgcttt gtttcactcc ttgaagatga
     1321 aacatccgtt gtgggaaggt cagttttcgc ccgttagggc gccggaaagt agccggtgtg
     1381 gaagcctgct ttgagg
//
LOCUS       DAOQDO010002038         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_111866_length_1396_cov_2.319165, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002038 DAOQDO010000000
VERSION     DAOQDO010002038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111866_length_1396_cov_2.319165"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1301)
                     /gene="asd"
                     /locus_tag="PKZ53_24765"
     CDS             complement(234..1301)
                     /gene="asd"
                     /locus_tag="PKZ53_24765"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNJ43717.1"
                     /translation="MATHSKKLRIGILGATGTVGQRFIQLLNEHPNFEITALAASDRS
                     EGKPYAQATNWKLSTPIPENIQTMVLAPCLPPLDCDVIFSSLPGNMAKETESLFAEQG
                     YPVISNSSSHRMADDVPLLVPEINADHVRLIDIQKRNRGFDRGFLVTNPNCTTLALVL
                     ALAPIERAFGIELGMISSLQAISGAGYPGVASLDILDNVIPFIDGEEEKVEIEPRKIL
                     GKLINTQISLAEMKLSAHCHRVAVSDGHTLAVSLKLRKPATMDEIRNVLESFQGPPQE
                     YRLPSAPDRVVVVRTERDRPQPRLDRDEGNGMSSVVGRLRQDPIWDVKFTVLGHNTVR
                     GAAGATILNAELLHHLGYFTA"
BASE COUNT          354 a          331 c          359 g          352 t
ORIGIN      
        1 gtgtcctggt gagagccggg ataggtgggt tcgattccca cacgcctccg ccaacgattg
       61 tgggtttagg gtttctggac cagcagaaaa agcccgttca attcattgaa ttgaacgggc
      121 ttttctttgg tctgggtgac agagaaacag aggataccgt ttggggatca caccttcgat
      181 ttatatgaag gctaaattat tgaaaaaaaa ttgtgtttcc ccaacccctg aacctaagcg
      241 gtaaaatagc caaggtggtg gagcaactca gcattgagga ttgtggcgcc ggctgcccca
      301 cggacggtgt tatgcccgag gacggtaaat tttacatccc aaatcgggtc ctggcggagc
      361 cgcccaacta cgctgctcat cccattgcct tcatctcgat ccaggcgtgg ctggggacga
      421 tcacgttcgg tacgaaccac aacaactcga tctggcgcac ttggcaaccg atattcttgg
      481 ggtggtccct gaaatgactc caacacattg cgaatttcat ccatcgtggc tggcttgcgg
      541 agtttcagcg agacggccag ggtatggcca tctgagaccg ccactcgatg acagtgggca
      601 ctgagtttca tctcggcgag cgaaatctgg gtgttgatga gttttcccaa aatttttctg
      661 ggttcaatct ccactttttc ttcttcacca tcaataaaag gaatgacatt gtccaaaata
      721 tcgagcgatg caacgccagg atatccagca ccagagattg cctggagtga tgaaatcata
      781 cccaactcaa tgccaaacgc ccgttcaatt ggggctaatg ccagaaccag cgccagcgtt
      841 gtgcagtttg gattggttac cagaaaccca cggtcaaatc cacggtttct tttttgaatg
      901 tcaatcaatc gaacatggtc agcattaatc tctggtacca ggagcggaac atcatctgcc
      961 atccgatggg aactggagtt gctaatgact ggatagcctt gctcggcaaa caggctttcg
     1021 gtttctttcg ccatattgcc cgggaggctt gaaaagataa cgtcacagtc aagcggtggc
     1081 aaacaaggcg ccagaaccat ggtttgaata ttttcaggaa ttggcgtcga gagcttccag
     1141 tttgtggcct gagcgtatgg ttttccttct gatcgatcag aggctgcaag agctgtaatc
     1201 tcaaaattgg ggtgctcgtt cagaagttga ataaaccgct ggccgacggt tccggtggca
     1261 cccagaatac caattctgag ttttttggag tgtgttgcca taataaattt gagtcaggtc
     1321 gaaaatagag tttggcgaaa cgactgggaa catccatcag ccgcagaaaa ataaacctga
     1381 tttcccagct tgagtc
//
LOCUS       DAOQDO010002039         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112149_length_1394_cov_3.206124, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002039 DAOQDO010000000
VERSION     DAOQDO010002039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112149_length_1394_cov_3.206124"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PKZ53_24770"
     CDS             complement(<1..709)
                     /locus_tag="PKZ53_24770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43718.1"
                     /translation="MITGINTDIKYNGVVYHVQTEDKGPENPLLLSLVYVGGHILAAK
                     RTNYARDLVDGISQDELQAKLDKQHKLILAIISRGRIDELVKMREREVQAEREAAQQT
                     SGSAEVPEPDRPMFSPPPVVPEAPVFSAPSPVLAEPVPAEVFPDITLPAAEAMPAAPP
                     LGTTQMLSPLLAEQFLSSFSSTPQPPIESAPSVGVSEELSALLGESSEPAGKITSPPA
                     PTPLGVTQMLSPLVAGLL"
     gene            1014..>1394
                     /locus_tag="PKZ53_24775"
     CDS             1014..>1394
                     /locus_tag="PKZ53_24775"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNJ43719.1"
                     /translation="MAPVEWNTSNAAHLLRRAGFAGSDVEIADLVKRGLEGSVDYLLN
                     YELIDNSALEKFLAETIRFDVEDPRSFNRNEIRRFFLLRMAYTRRPLEEKLTLFWHDH
                     FATAFSKVAEYPMYIQNLTLRRFAL"
BASE COUNT          338 a          347 c          370 g          339 t
ORIGIN      
        1 acagcaatcc agcaaccagt ggcgacagca tctgggttac ccccagtggt gtcggggcag
       61 gcggtgaggt gatctttccg gctggctcac ttgattcgcc cagtaatgcc gaaagttcct
      121 cagaaacccc gactgatggg gccgactcga taggaggctg cggtgttgaa gaaaagctgg
      181 aaagaaattg ctcagccagt aacggcgaga gcatctgggt cgtgcccagt gggggtgctg
      241 ctggcatcgc ttccgccgct ggaagtgtga tgtcaggaaa aacttcagcc ggcacaggtt
      301 cagccagaac aggtgatgga gctgagaaaa ccggggcttc tggaacaact ggcggaggtg
      361 aaaacatcgg gcggtcaggc tcaggaactt cggcgcttcc actcgtctgt tgtgcggcct
      421 cacgctcagc ctgaacctcg cgttcgcgca tctttaccag ctcatcaatt cgcccacgac
      481 tgatgatggc cagaattaac ttatgctgtt tatccaactt tgcctgtagt tcatcctggc
      541 taattccatc aaccaggtct ctggcatagt tggtccgctt ggcagccagg atatgaccgc
      601 caacataaac cagagacaac agcaacggat tttccgggcc tttgtcttcg gtttggacgt
      661 gatagacgac accattgtat ttgatgtcgg tgttaatacc ggtaatcatg gacggttacc
      721 actaaaaccg aaatcaggta gataggctga agttaggatc aatcacgaag agagttctgg
      781 atgtgctccg aaggtatcgc accataccat aggggttgaa agggcgtcaa cgcttcggga
      841 tttgctggat ttaggcagtt ttccaaacca tttccgtggt tggtgcgtca taggcggcag
      901 ttccgtcttt tcaaaagctc tggacatgta catccatatt attcatcgaa ctcatccacc
      961 taccagctta tcaaagctcc agaatatagc atgaagggag actgccagga cctatggcac
     1021 ctgtagaatg gaatacttca aatgcagcgc atctgcttcg ccgggcagga tttgccggaa
     1081 gcgatgttga aattgccgat ctggtcaaac gcggccttga aggcagtgtt gattacctgc
     1141 tcaattatga gttgattgac aacagcgcat tggaaaagtt tttggctgaa accatcagat
     1201 ttgatgtcga agacccgcgc tcgtttaacc gcaatgaaat ccgtcgcttt ttcctgttgc
     1261 gcatggcgta tacgcgccga ccgctggagg aaaaactcac cctcttttgg cacgatcatt
     1321 ttgcaaccgc gttttccaaa gtggctgaat atccaatgta tatccaaaac ctgaccctgc
     1381 gccggtttgc gctg
//
LOCUS       DAOQDO010002040         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112169_length_1394_cov_2.601942, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002040 DAOQDO010000000
VERSION     DAOQDO010002040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112169_length_1394_cov_2.601942"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKZ53_24780"
     CDS             complement(<1..235)
                     /locus_tag="PKZ53_24780"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNJ43720.1"
                     /translation="MIEAMRLEKTSTESAQASPDSVRSIFERACRGDEDAFRLIFNRY
                     ARPVMSFLHNLVNNHELAEELTQETFVRAHRMLN"
     gene            596..>1394
                     /locus_tag="PKZ53_24785"
     CDS             596..>1394
                     /locus_tag="PKZ53_24785"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43721.1"
                     /translation="MALFLGGWLSVSALDPKKELIDYSHAAYQDDLPQNTVHSIIQTK
                     DGYLWFGTYEGVARFDGVRFTIFDGSNTPQIRNNSIMSLAEGPDGTLWMATKGGGLVR
                     YQNHTFTSFTTENGLPSNFLITVCVDKQGRVWVGTQENGVVVYDGGKFQLFNTQNGLS
                     ANAINTLFCDHQGAMWIGTTGGGICRYFNNEFVSFSRESGLASTFVKAICEDQSRRVW
                     IGLEGGGITILDNGQSQTLTTADGLPSNVIRSLYCDREGSVWIGTEDR"
BASE COUNT          332 a          356 c          362 g          344 t
ORIGIN      
        1 tgttgagcat gcggtgagcc cgaacaaagg tctcctgggt gagttcttcg gccagttcgt
       61 gattgttcac cagattgtgg agaaaactca tcacgggccg ggcatagcgg ttaaagatca
      121 accgaaaggc gtcttcgtcg ccgcgacagg cacgctcaaa aatgctgcgt acactgtctg
      181 gcgaagcttg ggctgattcg gttgaggttt tttccaatct cattgcctca atcatgctgc
      241 ttcctcatcc ttggtttcgg ccagtgtgtg gccagacact cggaaaagtt gcagaaaaag
      301 ttgcaaagtg gggaaatttg ttccgaaaga atgcgaactg aagctccaaa acagcctgat
      361 ctggcggcag gtccaaaaac cacaattggt aatcaaggca caatttggct attatgcggg
      421 ttcaagacaa atccgcaaac ttttgagtca cttcctgcac cttgcccaga cggctgattc
      481 cttcgccacg gactccactc gctgacgcac gtttcgcctg aggctttcac cgatgtcgcc
      541 cacttcttgg actcaaaacc aattccgtct gtttggctgc cgatttttgg tgctgatggc
      601 tctgtttctt ggcgggtggt tatcagtcag cgccttagac cccaaaaaag aactgattga
      661 ttacagccac gctgcctatc aggatgacct gccacaaaac acggtgcatt ccatcattca
      721 aaccaaagat ggctatttat ggtttggcac ctatgaaggc gtggcgcgct ttgatggcgt
      781 ccggtttacc atttttgatg gcagcaatac gccacaaatt cgaaacaaca gcatcatgtc
      841 actggcggaa ggcccagacg gaactctctg gatggcaacc aaaggcggcg ggctggtgcg
      901 ctaccaaaat cacaccttta ccagtttcac gactgaaaac ggcctcccga gcaattttct
      961 gatcacggtc tgtgttgata aacagggacg ggtgtgggtt ggcactcagg aaaacggggt
     1021 tgtggtctat gacggcggga agttccagct tttcaatact caaaatggac tctccgccaa
     1081 cgccattaac accctgtttt gtgaccatca gggggccatg tggattggca ccaccggggg
     1141 cggaatctgc cgctatttca acaatgaatt tgtttcattt tcacgggaaa gcggcctggc
     1201 ttcgaccttt gtcaaagcca tttgcgaaga tcaatcccgg cgcgtatgga ttgggcttga
     1261 aggcggcggc atcacgattt tggacaatgg ccagtcacaa accctgacca cggctgatgg
     1321 attgccatct aatgttattc gctcactcta ttgtgatcgc gaaggcagcg tctggatcgg
     1381 caccgaagat cgcg
//
LOCUS       DAOQDO010002041         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112320_length_1393_cov_2.636024, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002041 DAOQDO010000000
VERSION     DAOQDO010002041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112320_length_1393_cov_2.636024"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..>1393
                     /locus_tag="PKZ53_24790"
     CDS             243..>1393
                     /locus_tag="PKZ53_24790"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ43722.1"
                     /translation="MLGSNPPVSPPQVSHPLAQVVNQARSLIELSPTAIVVMDLDSNI
                     YALNPAAERLLGYTTADVAGVLYPFVCPQDQEVLRQICTEVISDESVVKSTTSRIQKN
                     GNVCWVKATLATLSAQSGQVVGMIEFLEPVAPQDLFPSIETNSEPEPLTPEPRLTFSD
                     SMFESGPVVIFRCEAVSPHAILAITSNITQFGYTPQMFLSGEMASEDLVHPDDRERVW
                     ATVEVWKQCEYTPNLSEFYYRLVCADGSVRWVYDYTVPVRDETGTLIAFDGYLLDITS
                     QKLAEEALRESESRFRNIVNTLPQMVGLVGHDLRYQFVNNTYLEFFGLERSQIVGKTV
                     PEIIGRKVFAVTRPYIEQALIGHKVHFEQWLSLQDGKRCIEGYLIPHYS"
BASE COUNT          328 a          331 c          339 g          395 t
ORIGIN      
        1 caaggtttag aatgtatcac acctctcctt ccaaaaaaag cgacccccag tcaagtctgc
       61 tgccttgagg tatttccaat cccaaatact accttcagta gcaggcaata gatatatagc
      121 tatccctcat cgctatataa aacaaatggt tacgctcttt attcaataat ttgccgcaag
      181 tttgtacagg taacgctgtt attttcaaaa gcagctattc tggtcaagtt tttcatgcat
      241 ttatgctggg ttcaaatcca ccggtttccc ctcctcaggt ctcccatcct ctggctcagg
      301 ttgtgaatca ggctcggtcc ttgattgagc tttccccaac ggccattgtg gtgatggacc
      361 ttgactccaa tatttatgct ctcaacccgg cagcggaacg cctgttaggg tataccactg
      421 ctgacgttgc cggggtgctc tatccgtttg tctgcccgca agatcaagag gtcttgcggc
      481 agatttgcac tgaggtgatt tcagacgaat ccgtcgtcaa atccaccacg agccggatcc
      541 aaaaaaatgg taatgtgtgc tgggtaaagg ctacactggc gacgctttct gctcagtcag
      601 gtcaggtggt tggaatgatt gagtttttag agccggtggc ccctcaggac ctgtttccgt
      661 caattgaaac aaactctgaa cctgagcccc tgacgcccga gccccggctg accttttcag
      721 attcaatgtt tgaatctggc ccggtggtaa tttttcgatg tgaggctgtc tctccgcatg
      781 cgattctggc gatcacctca aatatcacac aatttggata cacccctcag atgtttttat
      841 ctggtgaaat ggcatctgag gatctggttc acccggatga tcgggagcgg gtgtgggcaa
      901 cggttgaggt ctggaaacaa tgtgagtaca ccccaaattt aagtgaattt tactatcggc
      961 tggtctgtgc ggatggttcc gtcagatggg tttatgatta caccgtacct gtgcgggatg
     1021 aaaccggcac cttgattgcc tttgacggat atcttctgga cattaccagc caaaaactgg
     1081 ctgaagaggc attgcgtgag agcgagagcc ggtttcgcaa catcgtcaat actctgccgc
     1141 aaatggttgg tctggttggc catgacctgc gctatcaatt tgtgaacaac acctatctgg
     1201 aattttttgg gcttgagcgt tcccaaatcg ttggaaaaac agttccggaa attattggtc
     1261 ggaaggtttt tgcggtgact cgaccttata tcgaacaggc actcattggg cataaagttc
     1321 attttgaaca gtggctctcg ctgcaggatg ggaaacggtg tattgagggc tatctaattc
     1381 cgcattattc tga
//
LOCUS       DAOQDO010002042         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112432_length_1392_cov_3.267764, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002042 DAOQDO010000000
VERSION     DAOQDO010002042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112432_length_1392_cov_3.267764"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..514)
                     /locus_tag="PKZ53_24795"
     CDS             complement(53..514)
                     /locus_tag="PKZ53_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ43723.1"
                     /translation="MENLERYLAEHSFFKDIEPQYLKLLVGCASNVRFQPGQFLFRCG
                     EEANSFYLIRHGRIAVELFAPNRGAITVQTVSEGEILGWSWLIPPYRWHFDAKATELT
                     RAIALDGKCLRTKCEADHSLGYELLKRFSYILSQRLEATSLQLLDIYGVGK"
     gene            complement(694..>1392)
                     /locus_tag="PKZ53_24800"
     CDS             complement(694..>1392)
                     /locus_tag="PKZ53_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNJ43724.1"
                     /translation="TDSRYQARSHNLFIVAVNCTVVGGTCFCASMQTGPKATAGYDLA
                     VTEILEADRHYFVVEAGTDAGMEVLNQIPHQTADPAETTRVDALMTQSASQMGRTLNT
                     ENIKDLLYRNSESPIWEAIAQRCLTCANCTLVCPTCFCSTVEDVTDLTGDIAERWRRW
                     DSCFTVEFSYIHGGPVRSSPESRYRQWMTHKLASWIDQFGTSGCVGCGRCITWCPVGI
                     DITKEVQTIRESEK"
BASE COUNT          340 a          345 c          368 g          339 t
ORIGIN      
        1 aagggcgtcg gaatcatcgg gtcattgtga taggaaattt gtacgttcga aatcatttcc
       61 cgaccccgta aatatcgagc agttgaaggc ttgttgcttc caggcgctgg ctgagtatgt
      121 aggaaaatcg tttcaacaat tcatacccca ggctgtgatc ggcttcacat ttggtccgca
      181 aacacttgcc atcgagggca atcgctcggg tgagttcggt tgctttggca tcaaagtgcc
      241 agcgataggg tggaataagc caggaccatc ccaaaatctc gccttcgctg accgtttgaa
      301 ccgtgatcgc acctcgattt ggggcaaata actcaaccgc aattctgccg tgtctgatca
      361 agtagaagct gttggcttct tcgccgcatc gaaacagaaa ttgccccggc tgaaaccgaa
      421 cgtttgaggc gcaaccgacc agtaatttca aatattgggg ctcgatatcc ttaaaaaaag
      481 aatgttctgc caggtatcgt tccagatttt ccatggacgc ctcctctaaa agtgatgaaa
      541 aaggacaaaa aggatttttt agggttcagg gttcagggtt cagggtttga agtactgagg
      601 tgactgatcc cactcaagat tgaggactgg ggaactacag ttaccgctca agtggagtat
      661 cttgctctga accctgaacc ctgaacccta aacttacttc tctgattccc gaatcgtctg
      721 aacctctttg gtgatgtcaa ttccgacggg acaccaggta atgcaccgcc cacagccgac
      781 gcagcccgaa gtcccaaact ggtcaatcca gcttgccagt ttgtgggtca tccactggcg
      841 atatcgggac tcaggtgatg agcggacggg accgccgtgg atataggaaa attcgacagt
      901 aaagcaggag tcccagcgcc gccagcgttc ggcaatgtcg ccagtcaggt ctgtcacgtc
      961 ttctactgtt gagcaaaagc acgttgggca gaccagtgtg cagttagcac aggtcaaaca
     1021 ccgttgggcg atggcttccc agatcgggct ttcagaattt cgatagagca ggtctttaat
     1081 attttcggtg ttgagggttc gccccatctg tgacgctgac tgggtcatca gggcatcaac
     1141 tctggttgtt tcggctggat ctgccgtttg atgtggaatc tgattgagaa cttccattcc
     1201 ggcgtcagtt ccggcctcga ccacaaagta atggcgatca gcttccaaaa tttcagtcac
     1261 ggccaggtca tacccggcag tggctttggg gccggtctgc atcgaagcgc aaaaacaggt
     1321 gccgccaacc acggtacaat tgaccgccac gatgaagaga ttgtgtgatc gtgcctgata
     1381 gcgggaatca gt
//
LOCUS       DAOQDO010002043         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112586_length_1391_cov_3.210329, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002043 DAOQDO010000000
VERSION     DAOQDO010002043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112586_length_1391_cov_3.210329"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKZ53_24805"
     CDS             complement(<1..523)
                     /locus_tag="PKZ53_24805"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNJ43725.1"
                     /translation="MLFTLLTIGSLLTPGLLLAAGKSEDEKFELLANSYIESFLKQNP
                     ETATLLGDHQFDGKLTDRSRSGYKQRLKVNVETLKSLGSINPARLSAINRIDYKILQS
                     NVEAKIFEIETLREHEWNPMLYNPGDALFSLVSRDFNPLKTRLESLKKRLESVPDVVA
                     SAKSNLQNPPKIYT"
     gene            complement(737..>1391)
                     /locus_tag="PKZ53_24810"
     CDS             complement(737..>1391)
                     /locus_tag="PKZ53_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNJ43726.1"
                     /translation="HGILVQLPLPPHIDAEAILRAVDPAKDVDGFHPVNAGKLLLKQP
                     GFVACTPAGIIEMLKFYDIPIRGSRAVVIGRSTIVGLPMSLLLLHRDATVTICHSRTK
                     DLPRVAQEADLLIAAIGRTAFVTEEHIRPGAVVIDVGINQVSTEADVAEYFGDDPTRQ
                     ADLAKKGYTLIGDVHPRHAIAKAAEFTPVPGGVGPLTIAMLMQNTVRAAEWISGIKL"
BASE COUNT          313 a          340 c          365 g          373 t
ORIGIN      
        1 cagtataaat tttgggcggg ttctgcaggt tggatttggc gctggcaacc acatcgggaa
       61 cactctcaag tcgctttttt aagctctcaa gccgggtttt gagtgggtta aaatccctgg
      121 agaccagcga aaataatgca tcgccggggt tgtaaagcat cgggttccac tcgtgttcac
      181 gcagggtttc aatttcgaaa atcttggctt cgacattgga ctgcaaaatt ttgtagtcaa
      241 tgcgattgat ggcactgaga cgggcagggt taatgctgcc gagtgacttg agcgtttcaa
      301 cattcacttt gaggcgctgt ttgtacccgc tacggctccg atctgtcagt ttgccgtcaa
      361 actggtgatc tcccaaaagg gtggccgtct cagggttttg ttttaaaaat gattcgatgt
      421 agctattggc aagtaattcg aatttttcgt cttccgattt tcctgccgca agcaacaaac
      481 cgggggtaag cagagaccca attgtcagaa gtgtaaagag cattttcatc ggtaaatcct
      541 tttttagtgc cgggtgcttg gtgcctggtt cttagtgctt ggttcttagt gcttggtgct
      601 tggttcttag tgcctggtgc ttggttctta gtgcctggtt cttggttctt agtgcctggt
      661 gcttggttct tggaagatat tgattcctaa gcactaagta cgaagtacca ggtacgaact
      721 atcagttacc taccacttaa agcttgattc ctgaaatcca ttcggcggcg cgaacggtgt
      781 tttgcatcaa catagcaatc gtaagggggc cgactccgcc tggaaccggt gtaaactcag
      841 cggctttggc aatcgcatga cgtgggtgga cgtctccgat taaggtatag ccttttttgg
      901 ccaaatcagc ctgtcgggtc gggtcatcgc caaaatactc agccacatca gcttcggttg
      961 aaacctggtt gatccccaca tcaatcacaa ccgcgcccgg tcgaatgtgt tcctcagtca
     1021 caaatgccgt tcgcccaatc gccgcaatca gcaaatcggc ttcctgggcc actctgggca
     1081 ggtctttggt gcgcgagtgg cagattgtca cggtggcatc acgatgcaac aacaggagcg
     1141 acatcggtag accaacaatg gtgctacgcc caatcaccac tgcccggctc ccacgaatcg
     1201 ggatgtcata aaacttgagc atttcaataa tgcccgccgg ggtgcaggca acaaatccag
     1261 gctgtttcag caacagcttc ccagcattga ctggatgaaa cccatccaca tctttggccg
     1321 gatccactgc ccggagaatc gcttcagcgt caatgtgtgg tggcaacggc agttgaacca
     1381 ggatgccgtg g
//
LOCUS       DAOQDO010002044         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112656_length_1391_cov_2.181138, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002044 DAOQDO010000000
VERSION     DAOQDO010002044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112656_length_1391_cov_2.181138"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKZ53_24815"
     CDS             complement(<1..591)
                     /locus_tag="PKZ53_24815"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ43727.1"
                     /translation="MNQNQHRVQCGVWGLAAMLLILPMLVPASKGQDSRGQRVSKANS
                     TRRVALVIGNAAYTEAPLKNPVNDARDMAAALREMGFEVTELHNQNFEQMETAIQTFG
                     QRLSGGGAGLFYFAGHGVQTKDNQNYLIPVGGTITAENVKYRAVNVGELTEQMQAAQN
                     GINLIILDACRNNPWRGLRSGARGLAAMDAPKGTLIA"
     gene            880..>1391
                     /locus_tag="PKZ53_24820"
     CDS             880..>1391
                     /locus_tag="PKZ53_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43728.1"
                     /translation="MFFRFNLLISLLVFLVGISVPASGQTKPDTISIASHNFKTGETS
                     ITAVSLTRTPQSTLPQIKILRGHSISTERGFQSNNFTESQLITSREDLLTFPNSAVVK
                     IRLEYPDRKEGSGGSGMLISPRHVLTARHVILGGENNDIWPSKAYVYPAFSTNDDPLP
                     FGESTGKSAFV"
BASE COUNT          303 a          364 c          326 g          398 t
ORIGIN      
        1 ggcaatcaac gttcccttcg gcgcgtccat cgccgccagt ccgcgtgctc ctgaccgcaa
       61 cccgcgccac ggattgttcc ggcaggcatc caggataatc aggttgatcc cattttgggc
      121 cgcttgcatc tgttcggtca gttctcccac gttcaccgcc cggtatttga cattttcggc
      181 ggtgatggtg ccgcccaccg gtatcaggta gttctggttg tctttggttt gtactccgtg
      241 cccggcgaaa tagaacagcc ccgcgccgcc gcctgacagt cgttgcccaa acgtctggat
      301 ggccgtttcc atttgctcga agttctggtt gtgcaactcc gtcacttcaa aacccatttc
      361 gcgcaatgcc gccgccatat cacgcgcatc gttgaccgga tttttgagcg gtgcttctgt
      421 ataggccgcg ttcccgatca ccagcgcgac ccggcgggtg gaattggcct tcgagactct
      481 ttgcccacga ctgtcctgtc ccttgctcgc cggtacaagc atcgggagta tcagcaacat
      541 cgctgccaac ccccaaactc cacattgaac gcggtgttga ttttggttca tggtgatttt
      601 cctcactctg gattttaatc ggcaacatct tgccagcatg ccggtttcga cactgtatca
      661 gatttggata gtccagaagc gcttgagaaa taatggttta tatttcttaa gcaccattgg
      721 gggagattgc tttttttcaa gatgattctg aaagtgatac accggattgg aaacaaactc
      781 aatagagtgc ttctgaaatt ggaagatctg caccaggttt gggcctgcca tctgctgttt
      841 aatcaatggg ttggtttttt ttgattcagg agtttaccta tgttttttcg attcaattta
      901 ctgataagcc tgcttgtgtt cctggttggt atttcggttc ccgcgtccgg acaaaccaaa
      961 ccggacacga tttcgattgc cagccacaac tttaaaaccg gggagacctc cattaccgcc
     1021 gtttcactca ctcgtacccc tcaaagcact ctcccacaaa tcaagatttt gagggggcat
     1081 tctatttcca cggaaagggg gttccaatca aataatttta ctgagagtca gctcatcaca
     1141 tctcgagagg atcttttaac ttttccgaat agtgctgttg taaaaatccg tcttgaatat
     1201 cctgacagga aagaaggctc aggcgggtct ggaatgttaa ttagtccacg tcatgtacta
     1261 actgctcgcc acgtaatttt gggcggtgag aacaatgata tttggccttc aaaggcttat
     1321 gtttacccag cttttagtac aaatgacgac ccccttccgt ttggtgaatc aactggaaaa
     1381 tcggcttttg t
//
LOCUS       DAOQDO010002045         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112747_length_1390_cov_7.029213, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002045 DAOQDO010000000
VERSION     DAOQDO010002045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112747_length_1390_cov_7.029213"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1219)
                     /locus_tag="PKZ53_24825"
     CDS             complement(95..1219)
                     /locus_tag="PKZ53_24825"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNJ43729.1"
                     /translation="MSDIEFPSSHAESRSFRRGLVMTALGVLVLIIGVFSLVKVLDSG
                     VRLYTQYHSVDGLHTGAEVRLAGVKIGKVKSFRFLDTTDVGPDTPQVEVEMLLDPTFQ
                     GKDLTSLIYKDSVVYLRTEGSLAQRILDIADGTPRSGTVKDGDLLKGQIDANLQRLSE
                     RGQTLTQTLEVAQTQLNEVGKNLKAGRGSVGAFLYRKEFQNRMLQLNQEVDSFVRELD
                     TGPGNIRKLRSPEFRKRLDRLSSLILKIADQVVAGKGTLGKAYQDQELANRLTRMAET
                     YQRLGQLVDKLNQRYKAGNGSIGKLAHDPKFQQDVRELQARIKRLSDGVKAGEGTIGK
                     YLQDPRLKNSLADTTTEAFKTLYDIQQSPRRYIRLRFSLF"
BASE COUNT          342 a          342 c          346 g          360 t
ORIGIN      
        1 ccaagaacca agaaccaaga accaagaacc aaatacattc ttcattcttc attcttcatt
       61 cttcattctt cattcttcat tcttcattct tcattcaaaa gaggctgaat ctcaggcgga
      121 tgtagcgtcg aggtgattgc tggatgtcat acagtgtttt gaatgcttcg gttgtggtgt
      181 cagccagcga atttttcaag cgtggatcct gcaaatactt accaatggtt ccttctccgg
      241 ctttgactcc atccgacagg cgtttgattc gagcctgaag ttcacggaca tcctgttgaa
      301 attttgggtc gtgggctaac ttgccgattg aaccgttgcc tgctttgtag cgctggttga
      361 gtttatcaac cagttgtcca agtcgttgat aggtttcggc catacgggtc agtcggttgg
      421 ccagttcttg atcctgatag gcttttccaa gcgttccttt cccggcgaca acctgatcgg
      481 caattttcaa aatcagggaa ctgagtcggt ccaggcgttt gcggaattcc gggctgcgga
      541 gttttcgaat gttgcccgga ccagtatcaa gttcccggac aaagctatca acttcctggt
      601 tgagctgcag catccggttt tgaaactctt tgcgatataa aaatgcccca acggagccac
      661 ggcccgcttt gaggtttttt ccaacttcat tcaactgagt ttgggcaact tcaagggttt
      721 gggtgagggt ttggccacgt tctgaaagcc gctgcaggtt ggcatcaatc tggcccttga
      781 gcagatcgcc gtccttgact gtcccgctcc gaggggttcc atccgcaata tcaagtattc
      841 gctgtgccag tgagccttcg gtgcgcaggt agacaaccga atctttatag atcaacgagg
      901 tcaggtcttt tccctgaaac gtagggtcaa ggagcatttc aacttcaacc tgtggcgtgt
      961 cagggcccac atccgtggta tcgagaaacc gaaaactttt caccttgcca attttgaccc
     1021 ctgccagccg gacttcggcc ccggtgtgga gtccatcaac cgaatgatat tgcgtataga
     1081 gccgaacgcc tgaatccaga accttgacca gtgaaaaaac cccaataatc aagaccagta
     1141 cgccgagcgc ggtcatcacc agtccgcgcc ggaaggatcg agattctgca tgagatgatg
     1201 gaaattcaat gtcagacatg cggatggttt agggtttggg gtttggggtt tggggaaaag
     1261 gcaaaaagac aaaaaggacc aaaatgacga aaaggacaaa aagaatatca aggtgctctc
     1321 gatttgaatg ataattcttt cgaaaacccg gaacccggaa cccggaaccc ggaacccgga
     1381 acccggaacc
//
LOCUS       DAOQDO010002046         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112769_length_1390_cov_3.041199, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002046 DAOQDO010000000
VERSION     DAOQDO010002046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112769_length_1390_cov_3.041199"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..745
                     /locus_tag="PKZ53_24830"
     CDS             260..745
                     /locus_tag="PKZ53_24830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43730.1"
                     /translation="MKQLIIGTLVTLILGITGVISAGQSQYKVYQNARFDYSISYPAK
                     ILFPQGEATNGDGQRFLSKDGKAELIVYGSHNALDQTLNDVFLDEQRQTEEHPRRTVT
                     YKLLKTDWFVVSGVDGETIFYQKTMLKHGIFKTFRITYPKNQKSTFDPITTVIAKSFR
                     G"
     gene            855..1265
                     /locus_tag="PKZ53_24835"
     CDS             855..1265
                     /locus_tag="PKZ53_24835"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ43731.1"
                     /translation="MSTPKKTPHPHFDDRGALSWHTKLADALAEAKATGKFVFIEFGR
                     FACGSCFALVETVLPQPPIAALLNEHFVCVASDCDHPEPEVMHIGRQHMPHARSLPFV
                     LYLDSNGNFLYGTTGGRSAEAFKADLDHVLQKKG"
BASE COUNT          369 a          352 c          335 g          334 t
ORIGIN      
        1 gcaccgggtg tctggtgggc caagttcagc cccgatgccc gcaacgtcaa ttacacgacc
       61 tctcaggcgg tgactgacat gggcgaaggc gcgacgtttt atgacaatct ggttgaagtc
      121 gccgtgcttg attaggagcc tcatttttca ccacagagga cacagaggat cacagaggaa
      181 attcgaaaat ggcatcgcgt ccctctattg tcctttgtgg tgagtttctg agttggtaca
      241 agctaaagag catctgtgta tgaaacaact gatcatcgga actctggtaa cgttgatact
      301 tgggatcact ggagttattt cggctggaca atctcaatat aaagtctatc aaaacgcccg
      361 ctttgactac tcgatttcat atccagcaaa aatcctcttt ccacaaggcg aagccaccaa
      421 tggcgatggt cagcggtttc tttcaaaaga tgggaaggcc gaattgatcg tctatggcag
      481 ccacaatgcg cttgaccaga cgctcaatga cgtttttctt gacgagcaac gtcagactga
      541 agagcatcca cgccgcaccg tgacctataa actgttgaaa accgactggt ttgtggtctc
      601 cggggttgac ggcgagacca ttttttacca gaaaaccatg ctgaaacatg gtatcttcaa
      661 aacttttcgg atcacctatc ccaaaaacca aaaaagcacc tttgacccca tcacgactgt
      721 gattgcgaaa tcatttcgag gctgaaaaac ctggggctaa ggggatgggg ctaagggctg
      781 agaaatcaat attttcgaat aaccctgaac cctgaaccct gaaccctgaa ctttgaacag
      841 aggaatcccc gtcaatgtcc acacctaaaa aaactcctca cccacatttt gatgatcgtg
      901 gagcactgag ctggcacacc aaactggctg atgccctggc agaagctaaa gcaactggaa
      961 agtttgtgtt tattgagttt ggccgctttg cctgtggcag ttgttttgcc ctggttgaaa
     1021 ccgtgcttcc ccagccgcca attgctgcgc tgctcaatga acactttgtc tgtgtggctt
     1081 cagattgtga ccacccggag ccggaagtaa tgcacattgg acggcaacac atgccgcacg
     1141 cccggtcgct cccgtttgtg ctctatctgg attcgaacgg gaattttctc tatggcacca
     1201 ctggtggtcg ttcagccgaa gccttcaaag ctgaccttga ccacgtactg cagaaaaaag
     1261 gctgaaaaat cctcgggctc agggcgattg aagggatgag cgatgaaaga tgaaggatga
     1321 aataaaacca atttttcaac caacgtcttc agccccgagc ttgcgagact tcagccctaa
     1381 gccctgagcc
//
LOCUS       DAOQDO010002047         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112926_length_1389_cov_3.339580, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002047 DAOQDO010000000
VERSION     DAOQDO010002047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112926_length_1389_cov_3.339580"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1175)
                     /locus_tag="PKZ53_24840"
     CDS             complement(42..1175)
                     /locus_tag="PKZ53_24840"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNJ43732.1"
                     /translation="MNLPNHQPLIGELIQFSNLTQLKRSAFFATHGAELDRIVTTPEL
                     FSPAVIHPPTQHFLRVACWNIERGNRFEAVRHLLTHHPLLSSADVLLLNEVDVGMVRT
                     GNRHVGFELAEAVGMHCVYGAEYLELTKGIGEELNLPGENTTALHGNAILSRYPLKQT
                     RLIQLPVCFEPFHFHEKRYGSRIALYAEIELSTGQRLALVTAHLEVRDTPACRGRQMQ
                     YILDWLRHNQLPVVFGGDWNTNTFPRGTRLRTAKSTARVLLTDPKALQHVLKHPDTGE
                     EPLFEALRASGFEWNDLNDDQPTCSVPLASMEDAALIPGLIRSWITHRIEQHAGHLHM
                     RLDWIASRGMKAASPGVTVADYWVDGQPASDHAPIVVDLTNEE"
BASE COUNT          355 a          359 c          324 g          351 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattcttc attcgttaga
       61 tccaccacaa tcggggcgtg atctgaggca ggctgaccat caacccagta atcagccaca
      121 gtcactccag ggctggcggc tttcattccc cgacttgcaa tccaatccaa tcgcatgtgc
      181 aaatggccag cgtgttgctc gatgcggtgg gtaatccagg atcgaatcag gccgggaatc
      241 agggccgcat cctccatgct ggccagcgga acggaacagg ttggctggtc atcattcaag
      301 tcattccatt caaatccaga ggcccgcaac gcctcaaata atggttcttc cccagtgtca
      361 ggatgtttta aaacgtgttg taaggctttg gggtcagtca acaacacccg cgcggtgctt
      421 ttcgccgttc gcaatcgggt cccacgtgga aaggtgttgg tattccaatc gcccccaaac
      481 accacgggta actgattgtg cctgagccaa tccaggatgt attgcatctg gcgaccacga
      541 caggccggag tgtcacgcac ttccagatga gctgttacca gcgcgagccg ctgcccggtg
      601 gataattcaa tttcagcgta caaggcaatt cggctgccat atcgcttttc atgaaaatga
      661 aatggctcga agcaaaccgg aagctgaatc aatctggtct gtttgagcgg gtatcgtgac
      721 aaaatggcat ttccatgcag cgccgtggtg ttctcacccg gtaaattcag ttcttcgcca
      781 atgcctttcg tcagttcaag atactctgcc ccgtacacac agtgcattcc aacagcttca
      841 gccagttcaa atcccacatg gcgatttccg gttcgcacca ttccaacatc aacttcattg
      901 agcaaaagta catccgcgct tgaaagcagt ggatgatgtg tcaacaagtg ccggacagcc
      961 tcaaaacggt tgccacgttc aatattccag caagccaccc gtaaaaaatg ttgcgtgggc
     1021 gggtggatca cggcgggtga aaacaactcg ggcgtggtga caatgcggtc cagttccgcc
     1081 ccatgagttg caaaaaatgc agaccgtttg agttgggtga ggtttgaaaa ttggatgagt
     1141 tcgccaatga ggggctggtg gttgggaagg ttcattgtgt ttcgccagat tgaggaattg
     1201 aaaagcaccg ccacagaata attgaacccg gtctcccgtc aataaattaa attaatccag
     1261 aatctaaaat tcaattaaat caaatagctt ttgaattgac tgtcctctct cgttttcaag
     1321 tgctccaata gagctaaaac tatctattct acaataactt acactaaaac ctggactatc
     1381 ccaggctgt
//
LOCUS       DAOQDO010002048         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112979_length_1389_cov_2.410795, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002048 DAOQDO010000000
VERSION     DAOQDO010002048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112979_length_1389_cov_2.410795"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKZ53_24845"
     CDS             complement(<1..483)
                     /locus_tag="PKZ53_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HNJ43733.1"
                     /translation="MAEERTYLELSEEGGSSHKFYEVVVDGAKLTIRYGRIGDAGQVQ
                     TKDFPTPEKATAEAQKKINEKKKKGYEPAVQGVRQKRTVTRRSAMLDLPPAPTAQPAR
                     SQSSRSSASSRSSAPARPLAPPPPRQTVPPRATSCPRVTKPIAHSIAHSHPPNSRSRP
                     N"
     gene            complement(649..1110)
                     /locus_tag="PKZ53_24850"
     CDS             complement(649..1110)
                     /locus_tag="PKZ53_24850"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ43734.1"
                     /translation="MRGRRLTLKPYLTSEQLEARFRLAPTCSEARHWLALWFISQGQS
                     VSDTAIATDLPKPHVQQLIYRFNNFGEEGVFKQRTPAAPGRPRRLTASQAHKIAQVLT
                     QSPPDGTAWTGRKVADWVWQELDLKISLTFANQLLRARVVPPARSSDSRKE"
BASE COUNT          306 a          318 c          425 g          340 t
ORIGIN      
        1 gtttgggcgc gatcgcgaat tcggggggtg ggagtgtgca atggagtgtg caattggctt
       61 ggtcacacgg gggcaggacg tggcacgggg tggcacggtc tggcgtggcg gcgggggtgc
      121 cagtggtcgg gccggagcac ttgaccgcga agaggcgctt gatcgtgatg attggctgcg
      181 ggctggttgc gcggttggtg ccgggggcag atccagcatg gccgaacgcc gggtgacagt
      241 gcgtttctgc cgcacaccct ggacggcggg ttcgtagcct tttttcttct tttcgttaat
      301 ttttttctga gcttcggcgg ttgctttttc aggagttgga aaatctttgg tttggacctg
      361 gccggcgtcg ccaatccgcc catagcgaat cgtgagtttg gcaccatcaa cgacaacttc
      421 gtaaaactta tgcgaactgc caccctcttc cgagagttca agataggttc gttcctcagc
      481 cataaagcct ccggtataga caagaaagta taaaatgaat cacacgtgcg atagcgattt
      541 acaagacaac tcagcggttg atcctgagcc tgccgaaaac acggcaagtg atacaggcaa
      601 cagaacatat ctgagttatt gagaaatttc aaagcaggtg gaatttttct attctttccg
      661 ggaatcagaa gaccgggcgg ggggcaccac acgtgcccga aggagctggt tggcgaaggt
      721 aagcgaaatt ttgaggtcaa gttcctgcca gacccagtcg gccactttcc ggcctgtcca
      781 ggcggttcca tcaggtggtg actgggtaag cacttgagcg attttgtggg cctggctggc
      841 agtcaatcgg cgggggcggc ctggggcggc tggcgttcgt tgcttgaaaa caccttcttc
      901 tccaaagttg ttaaaacgat agatgagttg ttggacgtgg ggtttgggca ggtcggtggc
      961 aatggcggta tcgctcacgc tctggccctg gctgataaac cagagggcga gccagtgtcg
     1021 tgcctctgaa caggttggtg cgaggcgaaa gcgcgcctct agctgttcag atgtgagata
     1081 gggttttaag gtaagtcgtc gtcctcgcat accgcaacgt atagggcacc ttgaattttc
     1141 ggacgacagg ttttcaatga attgtcgaaa tcgagcgacc atcaaaatct ttaaacaaat
     1201 gagctgagat tattcaaaaa caaattgaaa ttttggtgga accaactaca atcgcgctca
     1261 tcctttcatc agcgatatct ccccccattt tggtcacgcc tctttgttga aaaaccaaag
     1321 agttcagtct gctgccttga ctgggggtcg cttttttcag gaatccggga gcaggtggaa
     1381 gcgaaagcg
//
LOCUS       DAOQDO010002049         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_112980_length_1389_cov_2.397301, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002049 DAOQDO010000000
VERSION     DAOQDO010002049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112980_length_1389_cov_2.397301"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1389)
                     /locus_tag="PKZ53_24855"
     CDS             complement(<1..>1389)
                     /locus_tag="PKZ53_24855"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNJ43735.1"
                     /translation="LVVVDEAALALTGYRLTSPVESFYRDSSAYITTTAFRSHILLEA
                     PVESSDEELAQPWDEPGDFEATEKISRYQSPRHDPFADLRTTRRIGMDPIQLRTQFAP
                     LALFADSVVTDATGRASVKVKLPDSLTRYRVMAVAVAGARFGIGESTITAQLPLMVRS
                     SSLRFLNFGDQCEFPVLVQNQTARPLTVDVAIRANHLIFPQGTCQRVTVPANDRVEVR
                     FPVETTTPGTARVHIAGVAGQFSDAAEIVLPVYRPATTEAFAEQGSLEQGMIVQSLGA
                     LSTVLPDYGGVEVTTSSTQLQSLTDALLYLVNYEYACTEQLSSEILGVVVLRDVLSAF
                     QSRDLPDPKILDSNVAESLNKLKSRQLGTGGFCLWDSGNADFPFASIHALHAVAKAKE
                     KQYPVPDGLIERGKEYLRSIESVFKQEYSPESRQTMQAYALYVRHLLGESDVAQAREL
                     INHATPDQLPVEA"
BASE COUNT          326 a          378 c          371 g          314 t
ORIGIN      
        1 ggcctcgact ggaagctggt caggtgtggc gtggttgatc aattccctgg cctgggcaac
       61 atctgactcc cccagaaggt gacgaacata gagcgcatac gcctgcattg tttggcgcga
      121 ttcagggctg tattcttgtt tgaacacgct ttcaatcgag cgcagatact ctttcccccg
      181 ctcaatcaga ccgtcaggca cggggtactg cttctctttg gctttggcta cggcatggag
      241 cgcatggatg ctggcaaagg ggaaatcagc atttcctgaa tcccacaagc aaaatccacc
      301 agttccaagt tgtcgacttt tcaacttgtt caaactttca gcaacattgg aatcaaggat
      361 tttcggatcg ggtaaatcac gtgattgaaa ggcgctgagc acatcccgta acaccacaac
      421 gcctaagatt tcagatgaga gctgctcagt gcaggcatat tcgtagttga ccagatagag
      481 cagtgcatcc gtgagggact gcagttgtgt ggatgatgtc gttacctcaa caccgccata
      541 gtcgggaagt accgtagaca aagcaccgag cgattgcaca atcatgcctt gttccaggct
      601 gccttgttcc gcaaaggctt cggtggtggc tggcctgtac actggcaaaa cgatctcggc
      661 agcatcgctg aactgcccgg ccacccctgc aatatgaact cgtgccgtac ccggcgtggt
      721 ggtctcaacc ggaaatcgaa cttcaacccg gtcattggcc ggcaccgtga cccgctggca
      781 ggtcccctgt ggaaaaatta gatggttggc tcggattgcc acgtcaaccg tcaagggtct
      841 ggccgtctga ttttgaacca gcactggaaa ctcgcattga tctccaaaat tgagaaatct
      901 caaggatgat gatcggacca tcagcgggag ttgggccgtg atggttgatt ctccgattcc
      961 aaaccgtgcc ccagccaccg ccaccgccat cactcgatac cgggtgagcg aatcaggaag
     1021 tttgactttc acactggcac gtcccgtggc atcagtcacc acggaatcgg caaacaaggc
     1081 taatggcgca aactgggtcc ggagctgaat tggatccatc ccaatgcggc gagtggtgcg
     1141 cagatcggca aatggatcgt gtcggggtga ttgatacctc gatatctttt cagtcgcttc
     1201 aaaatcgccg ggctcatccc agggttgagc cagttcttcg tcacttgatt cgaccggtgc
     1261 ttccagcaaa atatgtgacc gaaaggcggt cgtggttatg tacgcactgc tatcccggta
     1321 aaaagactcc accggagaag taaggcgata cccggtcaat gccagcgcgg cttcgtccac
     1381 caccaccag
//
LOCUS       DAOQDO010002050         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_113101_length_1388_cov_2.994749, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002050 DAOQDO010000000
VERSION     DAOQDO010002050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113101_length_1388_cov_2.994749"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKZ53_24860"
     CDS             complement(<1..223)
                     /locus_tag="PKZ53_24860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43736.1"
                     /translation="MNSNPGSQIYFVRPHLAVCGFAGLINRQSFELHHFEAQLQCASG
                     FEPWLETCLEVKSLPLYDGYPVPADIFWEG"
     gene            complement(242..670)
                     /locus_tag="PKZ53_24865"
     CDS             complement(242..670)
                     /locus_tag="PKZ53_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ43737.1"
                     /translation="MKELKSCGILLFDSARYERFLLLRHASRYDLPKGHKDRGEDDLA
                     CALRETWEETGIESDQISLDDNFRCEIVYFPRYKRFCYEPVKKTLVMFLGHLTQDVDI
                     DLTEHFSFEWVKWAPPHQIQEQSIDQVLAEVEQYLKAKMF"
     gene            complement(706..1116)
                     /locus_tag="PKZ53_24870"
     CDS             complement(706..1116)
                     /locus_tag="PKZ53_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ43738.1"
                     /translation="MTNLELVQELYTAFGKRDTEAIRRIFHPEIEWIQNAGFPGGGTH
                     HGTETVLTEVFAKFRVEWETWQAMVTEWLDAGDNIIAIGVYRGTFKTTGRSMEAAFAH
                     VYTVQDGRIVKFRQYTDTLKVAEARQPAASLLEN"
BASE COUNT          349 a          347 c          336 g          356 t
ORIGIN      
        1 ggccttccca gaaaatatcg gctggaactg gatacccatc atacaacgga agcgatttga
       61 cttccagaca ggtttcaagc cagggctcaa acccggaggc acattgcaac tgggcttcaa
      121 agtgatgtaa ttcaaaggat tggcgattga tcaagcccgc aaacccacac actgccagat
      181 gggggcgcac aaaataaatc tggctgcctg gattgctatt catgaggaac acctcattcc
      241 atcagaacat ttttgctttc aagtactgtt cgacttcagc cagtacctga tcaatgcttt
      301 gttcctgaat ctgatgtggt ggcgcccact tgacccactc aaagctgaaa tgctcagtga
      361 gatcaatatc cacgtcctgg gttaaatgtc ccaggaacat caccagcgtt tttttcaccg
      421 gttcgtaaca aaagcgtttg taccttggga agtacacgat ttcacatcga aaattgtcat
      481 cgagggaaat ttgatcagat tcgatcccgg tttcttccca ggtttcgcgc agggcacagg
      541 cgaggtcatc ttcgccacgg tctttgtggc ctttgggcag gtcgtaccgg ctggcatgcc
      601 gcaggagtaa aaaccgttcg taacgggccg aatcaaacaa caagatgcca caggatttca
      661 gttctttcat atggtgtagg gttcggggtt cagggttcag ttcaatcagt tttccaaaag
      721 tgaagctgct ggttggcggg cttcggcaac tttgagtgtg tcagtgtact ggcggaactt
      781 cacaatacga ccatcctgca cggtgtagac gtgcgcgaag gcggcttcca tcgaacgccc
      841 cgtcgttttg aatgttcccc gatacacgcc aatagcaata atgttatcac cggcatcaag
      901 ccattcggtc accatcgcct gccaggtctc ccactcgacg cgaaatttgg caaacacctc
      961 cgtcagcacg gtttctgttc cgtgatgggt cccgccgcca ggaaaccctg cgttttgaat
     1021 ccattcgatt tcgggatgaa aaatccggcg aatggcttcg gtatcgcgct ttccaaacgc
     1081 ggtgtagagt tcctgtacca gttcaagatt ggtcattggt agttcgctcc aaaatgggtt
     1141 gtttggtgtc agtggataaa tccagggccg gtttctgatt tgatttccgt agaaacaata
     1201 ctcgtaaaat cttctcatcg aacaaaatac atcaagtcag aaatctatct ttccaatccg
     1261 tgacgactga cgactgactg atcaactact gactattgat caagcttcgg ttagaatagc
     1321 cacctcggtt tcacaaactc aactgaacgg agcggaaagg agggaagcga tgggcgcgtt
     1381 tacttttc
//
LOCUS       DAOQDO010002051         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_113264_length_1387_cov_2.975976, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002051 DAOQDO010000000
VERSION     DAOQDO010002051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113264_length_1387_cov_2.975976"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1387)
                     /locus_tag="PKZ53_24875"
     CDS             complement(23..>1387)
                     /locus_tag="PKZ53_24875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43739.1"
                     /translation="FYSFSLADSLITELAALKSLVVRPSSFIAKFQGREIDAQKVGAE
                     LKVDAVLISGYLKAGNRFRVTPQLIDINTGEILWSDKIDLDAADIMTLQDTISRKIVD
                     ELRVRITETEQVRIATPATNNSEAYEYYLRGRNQLNRFMQTMMKDDFSSAIGMFENAL
                     RLDPNFALAHSGIGLCYVQYVLKGIGGSVHFERAEGRFREAMKLDDSLIEPQSNMVYI
                     HLLKNEKIEARAQVKRVLSVAPNDANVHSIAGYLYRWDGLYKQALQQYDTCIKLNPTD
                     LVPAAYNRARILMYQGRYEDAMNELESALFYEPDHPFTLMVLGQVQYYQGQWEEASTT
                     LKNVLDRNPDLHAFRPIYGLCHVATGPPEKALAIINDAVRDTASADGDAAYWLATVYA
                     ALKRESEALQWLRKAITLGNENYPWFCNNPDWKDFQDNPKFKEILEPLRVQWLDLCRQ
                     YGNP"
BASE COUNT          364 a          356 c          304 g          363 t
ORIGIN      
        1 tcattcttca ttcttcattc aatcaggggt tgccgtactg gcggcacagg tcaagccatt
       61 ggactcgcaa tggctccaaa atttccttaa atttcggatt atcctgaaag tctttccaat
      121 ccgggttatt gcaaaaccag gggtaatttt catttcccaa agtaattgcc ttgcgcagcc
      181 actgtaaggc ttcactttcc cgtttcagcg ccgcataaac cgtagctaac cagtatgcag
      241 cgtcgccatc ggcactggcg gtatcacgaa cagcatcgtt aataatggcc agtgcttttt
      301 cagggggacc ggttgccacg tggcacaagc cataaatggg gcgaaacgcg tgcaggtcag
      361 ggttccggtc aagcacattt tttaaggtgg ttgacgcttc ttcccattgg ccctggtaat
      421 actgaacctg gccaagcacc atcaaggtga aaggatgatc tggttcataa aacagcgcgc
      481 tttccaattc attcatcgca tcttcgtatc gtccctgata catcaaaatt cgggcacgat
      541 tgtatgcggc aggtactaaa tcagttggat tcagcttgat acaggtatca tactgctgga
      601 gtgcctgttt ataaagacca tcccaccgat atagatatcc agcaatactg tggacattgg
      661 catcatttgg agcaaccgaa aggacccgtt tgacctgagc ccgagcttcg atcttttcat
      721 ttttcaaaag atggatgtac accatgttcg attggggctc aatcaatgag tcatccagct
      781 tcatagcctc acggaaccgt ccttcggccc gttcaaaatg gaccgaaccg ccaattcctt
      841 ttaaaacata ctggacataa cacaacccga ttccagaatg agccagggca aagtttggat
      901 ccagacgcaa cgcattttca aacatgccaa tcgccgatga gaaatcatct ttcatcatgg
      961 tttgcataaa ccgattcagt tgatttcgtc cccggaggta atactcatac gcttcggaat
     1021 tgttggttgc cggtgtggca atccgaacct gttccgtctc agtgatgcga acccgaagtt
     1081 catccacgat tttgcgggaa atggtgtcct gcaaggtcat gatatcagcc gcatcaagat
     1141 caattttatc gctccacaag atctcgccgg tgttaatatc aatcaattgc ggcgtcaccc
     1201 gaaagcgatt cccggctttg agatagccac tgatcaacac ggcatccact ttgagttcag
     1261 cgcctacctt ttgggcatca atttcccgtc cttgaaactt ggcaataaaa ctggacggtc
     1321 tgaccaccag cgatttcaag gcagccagtt cagtaatcaa gctatcagcc agcgagaaac
     1381 tataaaa
//
LOCUS       DAOQDO010002052         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_113310_length_1387_cov_2.219219, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002052 DAOQDO010000000
VERSION     DAOQDO010002052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113310_length_1387_cov_2.219219"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..>1387)
                     /locus_tag="PKZ53_24880"
     CDS             complement(72..>1387)
                     /locus_tag="PKZ53_24880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ43740.1"
                     /translation="TTMYRKTTLENGVRIITETIPEVRSTSIGVVLNAGPRTEMPSQC
                     GLAHLTEHLMFQGTSSRSALQIARLMDGAGGYMGGFTTQDYTYYSATVLAEYSTYVLD
                     LFGDILLNSIFPQDSLEKEKEAVICEIEAARDNPDQRVRQLLRATAWPDHPLGRPVAG
                     YTEAVKTLTREDVIYFAHQHYLPTNLIVAAAGQLDHDDFVAQVRDAFWRMLGDGSTPT
                     VAPPAPKMSIVTENSPVSQAYFCIGLRTFPYTHPDRYALHVLDKVLGGGISSRLFRRI
                     REELGLVYYLGSEYLAFLEDGMLVIEGSTSPEYLQMVLELTLAELTELFTWETPVTDE
                     ELLIAQTHLRGQHLISGENTDTRMSRLATQELYFGRHIPTDEIAAHLMAVDLPALQRL
                     GQTVLVDALRQATIAVVGPDVSESCNVPRLEKQLSLFDSTILQRV"
BASE COUNT          333 a          356 c          377 g          321 t
ORIGIN      
        1 gtaaaggtcc tccatgaaaa aataaaataa gcttgtacag ttccgctgta ccgggttaag
       61 ccccaattgg ctcaaactcg ctgcaaaata gttgaatcaa aaagagaaag ctgtttttca
      121 agacgcggca cattacaact ttccgataca tcagggccga caaccgcgat ggttgcctga
      181 cgcagggcat ccaccagcac cgtttgccca agccgttgca gtgccggcag atcaacagcc
      241 atcaggtgag ccgcgatttc atcggttgga atatggcgcc caaagtacaa ttcctgggtg
      301 gccaggcggc tcatgcgggt atcggtgttt tcgccagaga tgaggtgctg cccgcgcaaa
      361 tgtgtctgag caatcaggag ttcttcgtcg gtgacaggtg tttcccaggt gaagagttcg
      421 gttaattcgg ccagggtcag ttccagcacc atctgcaaat attcaggcga ggtgctgcct
      481 tcgatgacga gcatgccatc ttcgagaaag gccagatatt cagatcccag gtagtacacc
      541 aggccaagtt cttcgcgaat gcgccgaaac aatcgcgaac tgatgccgcc gccaagcact
      601 ttatcgagca cgtgcagtgc ataacggtct ggatgggtat atggaaacgt ccgcaatccg
      661 atgcagaaat aagcctggga caccggtgag ttttcagtca cgatgctcat tttcggggca
      721 ggcggggcga cggtgggtgt cgagccgtct ccgagcatcc gccagaaggc atcgcgaacc
      781 tgagcaacaa aatcgtcgtg atcaagctga cctgccgctg ccacaatcag attggtgggc
      841 aggtaatgtt gatgcgcaaa ataaatcaca tcttcgcgcg tcagtgtttt aacggcctca
      901 gtgtatcccg ccaccgggcg tcccagcgga tgatctggcc aggctgttgc tctgagcaac
      961 tggcggacgc gttgatcagg attgtcacgg gcggcttcga tttcacagat gacggcttct
     1021 ttttcttttt caaggctgtc ctggggaaag atcgaattga gcaaaatatc gccaaagaga
     1081 tcaagtacat aggtactgta ttcggccagt accgttgctg aataataggt gtaatcctgg
     1141 gttgtaaacc cgcccatata tccgcccgca ccatccatca accgtgcaat ctggagcgca
     1201 cttcggctgc ttgtcccttg aaacatcagg tgttcggtca ggtgtgccag cccgcattgg
     1261 ctgggcattt cggtacgtgg accagcattg agcacaactc caatcgaagt tgaccgaacc
     1321 tcaggaatcg tttcggtgat aatgcgaacc ccattttcga gcgttgtttt ccgatacatg
     1381 gtggttg
//
LOCUS       DAOQDO010002053         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_113600_length_1385_cov_2.351128, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002053 DAOQDO010000000
VERSION     DAOQDO010002053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113600_length_1385_cov_2.351128"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>1385
                     /locus_tag="PKZ53_24885"
     CDS             102..>1385
                     /locus_tag="PKZ53_24885"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNJ43741.1"
                     /translation="MAHFVAHLRFSSVNVMKFLSLLRLILSGLVGLWFVWASPQTLLA
                     RTGPVPVRLVNAKADLNEFKWKFQPGDDPTWADSRFDDSPWKEISTTESWVKQGHSGY
                     SGYGWYRLKAQLPTEQIPTAPQRALALTLTPPRTSEGEVFIQGRTVGNWGKSPAPLFS
                     LDRLEGPQVFSIPSELIPADGILVIAVRFYWSPQAAQYFPRQGGFQGGRFQLGEPTLL
                     TELAIAYKRNRLINYLPQLLFAAIFVVAGLYHLLLFWRRRQLREYLWFGLILLASAIN
                     NLALTPWAEFLLPPFVGNILTNCTLYLIFMGMLPFTLSFLGFRFHRLACITEGLLGLC
                     LVLTLLFPASIVTNPQAALVDFCLLMSVMVWCLGLVIRESWRGNPDARTILFGSIITT
                     AFEVYLLIAPIGVVPFFILQYVGLAVFMFSMAAALA"
BASE COUNT          272 a          362 c          352 g          399 t
ORIGIN      
        1 gatttcgaca tgtgctcagt ccttcccaat ggtctgaagt gcgcctgctg ttatcgaaag
       61 tgtgattcat tgacggtttg ccgaaaatct ttattctgtg gatggcacat ttcgtcgccc
      121 acctgcggtt ttcatcggtc aatgtcatga aattcctgtc gctacttcgt cttatcctca
      181 gtggattggt tggactctgg tttgtctggg cttcacctca aacgctgctg gcacgtaccg
      241 ggccagttcc agtcagactc gtcaatgcca aagccgatct caatgaattc aaatggaaat
      301 tccagcccgg agatgaccca acctgggccg acagccgttt tgacgactcc ccctggaaag
      361 aaatttcaac cactgaaagt tgggtcaaac agggacatag cggatatagc ggatatggct
      421 ggtatcggtt gaaagcccaa ctgcccacag aacagatacc cacagcgcct caacgcgcat
      481 tggcacttac actgacaccg ccccgaacca gcgaaggtga ggtgtttatt caggggcgca
      541 cggttggaaa ttgggggaaa tcgccagcac cgcttttttc acttgaccgg ttggaaggtc
      601 cccaggtgtt ttcaatcccg tcggaactca tcccggcaga tgggatactg gtcattgcag
      661 ttcgctttta ctggagtccc caggccgctc aatattttcc gcgacagggc gggtttcagg
      721 gtggtcggtt tcaactgggc gaaccaactt tgctgaccga actggccatc gcctacaaac
      781 gaaaccggtt aatcaattat ttgcctcaac ttctctttgc tgcgattttc gttgtggccg
      841 ggctctatca cctgttgctt ttttggcgac ggcggcaact tcgtgaatac ctgtggtttg
      901 ggctgattct gctggcttcg gcaatcaata acctcgcgct cacgccctgg gccgagtttc
      961 ttcttccacc atttgtcggc aacatcctca ccaactgcac gctgtatctg atctttatgg
     1021 gcatgctgcc gtttacgctg tcgtttctgg gttttcgctt tcaccggctg gcctgcatca
     1081 cagaaggctt gctgggattg tgtctggtgc tcaccctgct cttcccggca tcaatcgtca
     1141 ccaatccaca ggcggcgctg gttgattttt gtttgctgat gtcggttatg gtttggtgtt
     1201 tagggcttgt catccgggaa agctggcgtg gaaatccaga cgcccgaacg attctttttg
     1261 gaagtatcat caccactgcg tttgaagttt acttactgat tgccccgatt ggtgttgtac
     1321 cattttttat tctgcaatat gtggggttgg ccgtttttat gttttcaatg gcggcagcac
     1381 tcgct
//
LOCUS       DAOQDO010002054         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_113714_length_1384_cov_3.403311, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002054 DAOQDO010000000
VERSION     DAOQDO010002054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113714_length_1384_cov_3.403311"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKZ53_24890"
     CDS             <1..220
                     /locus_tag="PKZ53_24890"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNJ43742.1"
                     /translation="SDPWFSVGENDIFPEEFQNFLQLTGKQRDVFLHLHGDLFQVKFW
                     RSIQDRLRAGEVIRILPYLPEKSLGEMM"
     gene            335..583
                     /locus_tag="PKZ53_24895"
     CDS             335..583
                     /locus_tag="PKZ53_24895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43743.1"
                     /translation="MVARLWALPPDHRPIPLRARMGAGSSVGFFSGWIPILTDQFGSS
                     AHPGENRVDSIKEPNSIPHLWGVVLSDSRSLQFTFQPG"
     gene            complement(554..988)
                     /locus_tag="PKZ53_24900"
     CDS             complement(554..988)
                     /locus_tag="PKZ53_24900"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ43744.1"
                     /translation="MIRKGSARWSGTIKEGSGTVSTPSGTLNDTPYSFTSRFEDGSGT
                     NPEELLAAAHAGCFTMAFSLMLTMKGFTPGTLSTNATLTMERQEGGFTITKVHLDLVG
                     AVPGIDDETYQALAAQAKANCPVSKLFNAEITLSARLEGELQ"
     gene            complement(1112..>1384)
                     /locus_tag="PKZ53_24905"
     CDS             complement(1112..>1384)
                     /locus_tag="PKZ53_24905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43745.1"
                     /translation="DSPIFPLSKIEFAIAGNIELVKAKQLTIQYLAINDGLEDILYTL
                     KNQYHVVRLVKRYPQLFLYLVLNRKKSNLAMARLILSRIEEKLVLE"
BASE COUNT          345 a          338 c          358 g          343 t
ORIGIN      
        1 atctgacccg tggtttagcg tcggcgaaaa cgatattttt cccgaggaat tccaaaactt
       61 cctgcagttg accggaaagc agcgcgacgt ctttcttcac ctccacggcg acctgtttca
      121 agtcaaattc tggcgatcta ttcaggatcg gttgcgggct ggcgaggtca ttcgaatctt
      181 gccgtacctg ccggagaaaa gcctggggga aatgatgtga gtaaccgaac gagcgagtag
      241 acgagtaaag catcgcacca gagcaagtcc ccataaaaaa gggatgaaca aagatctgat
      301 gccctaccgc ccggagggcg atggagagta gccggtggtc gcgcggcttt gggcgctacc
      361 accggatcac cgccccatcc cgcttcgcgc ccggatgggc gctggaagtt cagtcgggtt
      421 tttctccggc tggattccga tcctgaccga ccagtttggt tccagcgccc atccgggcga
      481 aaaccgggtt gattcgatca aagaaccgaa ttccatccct catttatggg gagttgtact
      541 tagcgactca cgctcactgc aattcacctt ccagcctggc tgaaagtgtg atttcggcat
      601 taaacagttt tgaaaccggg cagttggctt tggcctgggc ggcaagagcc tgataggttt
      661 cgtcatcaat tccaggcacg gcgccgacta agtccagatg aactttggta atggtaaacc
      721 cgccttcctg tcgttccatc gtcagggtcg cattggttga aagggtgcca ggtgtaaacc
      781 ctttcatcgt caacatcaag gaaaatgcca tggtgaagca cccggcgtgg gccgctgcga
      841 gcaattcttc agggttggtg ccggagccat cttcaaaccg gctggtgaat gaataggggg
      901 tatcgttgag tgttccgctt ggagtggaaa cagtaccact gccttcttta atcgtacctg
      961 accaacgagc tgaacctttg cgaatcatgt gaaaaactcc tgtgaaggga ttaaatattg
     1021 aactatttgg aaacttgtct ccaaaagact ggcacacctt agaaagaatg tggttgtcag
     1081 gtcaatctga accggaaact ttctgagaat ctcactctaa aaccagcttt tcctcaattc
     1141 ttgaaagaat cagtcgcgcc atcgccagat tggatttttt tcgattgagg accaaataca
     1201 ggaataactg cggatagcgt ttgaccaacc gcaccacgtg atactggttt ttgagtgtat
     1261 ataagatatc ttccagacca tcgttgatgg ccagatactg aatcgtcaat tgtttagctt
     1321 tgaccagctc aatgtttccg gcaatcgcaa attcaatttt tgaaagcgga aatatggggg
     1381 aatc
//
LOCUS       DAOQDO010002055         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_113756_length_1384_cov_2.434913, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002055 DAOQDO010000000
VERSION     DAOQDO010002055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113756_length_1384_cov_2.434913"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKZ53_24910"
     CDS             <1..429
                     /locus_tag="PKZ53_24910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43746.1"
                     /translation="LVKLEPQNLEYRTGLGVWAYRFQQYQEAVDNLAYAYRQDPHFKE
                     NASNCFALGYSLLVTRHPKESLDPLMQAVKIDAQIPQYWYTLGDARLDLGQPVEALEC
                     YYTVLKLDASQPEAVKSKITLVEQSLHQVPQKTKLPGSHS"
     gene            499..1071
                     /locus_tag="PKZ53_24915"
     CDS             499..1071
                     /locus_tag="PKZ53_24915"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNJ43747.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELNPNIEVHRNDRLTLFDINQKSP
                     SHIVISSGSGHPQRAGISVEAIQTFAGRIPILGVGLGIQALVVAFGGTVAPTPVITLG
                     KTSEICHDGRSVFQGLEYRFRATRYHSLMVDRNYIPPVLEVSATTPDGIIMGLRHRQA
                     ICEGIQFHPESIMTLPGKVLIRNFLSLSTV"
     gene            1126..>1384
                     /locus_tag="PKZ53_24920"
     CDS             1126..>1384
                     /locus_tag="PKZ53_24920"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNJ43748.1"
                     /translation="MPSYGRLLNSLAFLFFLIVFGTVTFHLLEGWSLLDSLYVTVITL
                     ATVGYGDFVPKTWEGRLFTIMFVLVGVGGAGYVITTAAQTVL"
BASE COUNT          317 a          345 c          324 g          398 t
ORIGIN      
        1 ctggtgaaat tagagcccca aaatctagag tatcgaacag ggttgggtgt atgggcctat
       61 cggtttcaac agtaccagga agccgttgac aacttagcct atgcctatag acaagaccct
      121 cactttaaag aaaatgcctc aaattgcttt gcgctcggtt attccttgct tgtaacccga
      181 catccaaaag aatcactgga cccactgatg caggccgtta aaatagacgc tcaaattcca
      241 caatactggt acactttggg cgatgcccgg ttggacctgg gtcagccagt cgaagccctt
      301 gaatgctact acaccgtatt aaaactggat gccagccagc ccgaggcagt caaatcaaaa
      361 atcaccctgg ttgagcagtc gctgcaccag gttcctcaaa agaccaaact gcccgggtcc
      421 cattcttaat ctttgattga cttgaggtct accccaccct ggtcaatttc gccagccatt
      481 tttttaggtg agatttttat gatccttgtg attgataact acgattcctt cacctacaac
      541 ctggttcaat atctcggcga actgaatcca aatattgaag tccatcgcaa tgaccgcctt
      601 accctcttcg atattaatca gaaaagcccc tctcacattg tgatttcatc tggaagtggt
      661 catccccagc gggctggtat atcggtcgaa gccattcaaa cctttgctgg gcggattccg
      721 attttgggtg tcgggcttgg gattcaggct ctggtcgtag cttttggagg aactgttgcc
      781 ccgaccccgg tgattacgct tgggaaaacc agtgaaattt gtcacgacgg gcgaagtgtt
      841 tttcagggcc ttgaataccg gtttcgggca actcgctatc attcgctgat ggtggaccga
      901 aattatattc cgccagtgct tgaagtctct gccacaaccc cagacggtat cattatgggg
      961 ttgcggcatc gtcaggcgat ttgcgaaggg attcagtttc atccggagtc aattatgaca
     1021 cttcctggta aagtattgat tcggaatttt ttaagtctat caactgtcta atcttcaatt
     1081 gattgtcacc tccgattcgt tcacaagcac tcactttgtt ttgttatgcc gtcatatggg
     1141 cgattgctca actccctggc ttttttattc tttcttattg tattcggcac ggtcacgttc
     1201 catctgctgg aaggctggtc actgcttgat tcgctctatg tgacagtcat taccctggcg
     1261 accgtcgggt acggagattt tgttcccaaa acctgggagg gtcggttatt tacgatcatg
     1321 tttgtgctgg tcggggtcgg cggcgccggc tatgtgatta cgactgctgc ccagacagtg
     1381 ctcc
//
LOCUS       DAOQDO010002056         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114102_length_1382_cov_2.097212, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002056 DAOQDO010000000
VERSION     DAOQDO010002056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114102_length_1382_cov_2.097212"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1382
                     /locus_tag="PKZ53_24925"
     CDS             62..>1382
                     /locus_tag="PKZ53_24925"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNJ43749.1"
                     /translation="MNHPLLTFALDWIDERTGLRHLMSEALDEPIPGGSSYAYTFGSA
                     LVFVFMLQLLTGMFLTMYYVPSAEDAHRTVEYISKEVMCGAFLRGIHHYSASAMVILV
                     VCHILQIIIWGAYKKKRELVWLVGAGLLQFILAFSLTGYLLPWDMKAYFGTQVTVNIA
                     SEVPLLGDFIKRVIQGGPTLGTLTLSRFFMVHAFLLPALAIGGIGLHLFLFRKARPAG
                     PFHLSEDQANARTETFFPGQLFKDAFVALVIFGVIAWLAWTAPAPLEPKANPGNTAYI
                     ARPEWYFLSLFQLLKYFQGGLAIVGSLVIPGALISVLTFLPFIDRSPERNPFKRPVLM
                     SCTAAVLATIGILTFMAKSEDRATYAEQFAKQARDGQEFLKQPFKPKEIGGAPVAPAA
                     KKPVPEAFKTKCAMCHGDTGRGGPVGPNLAGISRTKEDIIGLIENPGA"
BASE COUNT          289 a          358 c          373 g          362 t
ORIGIN      
        1 ttcttcagcc cgatttcttc agcccgattt cttcagcccg atttcttcag cccgaatcct
       61 tatgaaccat cccctcctta cctttgccct ggactggatt gatgaacgga ccggtttgcg
      121 ccacttgatg agcgaggcgc ttgacgaacc gattccgggc gggtcgagtt atgcctacac
      181 gtttggcagt gcgctggtgt ttgtgtttat gctgcaactg ctgaccggca tgtttttgac
      241 tatgtactat gttccgagcg ccgaagacgc ccaccgaacg gtcgaataca tcagcaaaga
      301 agtgatgtgc ggtgcctttc tgcgtggaat ccaccattac tcggccagtg ccatggtgat
      361 tctggtcgtg tgtcacattt tgcagatcat catttgggga gcgtacaaaa agaaacgcga
      421 actggtgtgg ctggttgggg caggtcttct gcagtttatt ctggcctttt cactgaccgg
      481 atatctgctt ccgtgggata tgaaagccta tttcggcacc caggtgacgg tcaacattgc
      541 cagcgaagtc ccgttgcttg gagattttat caaacgggtg atccagggcg ggccgacgct
      601 tgggacgctg acactgtcgc gcttttttat ggtccatgcg tttttgctcc cggcgctggc
      661 aattggcggc atcgggttac atttgtttct gtttcgcaaa gcacgcccgg ctggaccctt
      721 tcacctttct gaagatcagg ccaatgctcg gaccgagacc ttttttcctg gtcagctttt
      781 taaagatgcg tttgtggcgc tggtgatttt tggcgtgatt gcgtggctgg cctggacggc
      841 accagcacca cttgagccga aagccaatcc gggaaatacg gcctacatcg cacgccccga
      901 atggtacttc ctgtcgcttt ttcaattgct caaatatttt cagggaggac tggccatcgt
      961 tgggtcactg gtcattcctg gcgctcttat cagcgtgctg acattccttc cgtttattga
     1021 tcgaagtcca gaacgcaacc ctttcaaacg cccggtttta atgagctgca cggcagcggt
     1081 tttagcaacc attgggattc tgacctttat ggccaaatcc gaagatcggg ccacctatgc
     1141 cgaacagttt gccaaacaag ccagagacgg gcaggagttt ttaaagcagc cgtttaaacc
     1201 caaggaaata ggtggcgcac cagttgcacc agccgcgaaa aagccggttc cagaagcctt
     1261 caagacgaaa tgtgcgatgt gtcacggaga tacaggtcgg ggtggcccgg tggggccgaa
     1321 tctggcggga atttcacgaa ccaaagaaga tatcatcgga ctcattgaaa accctggtgc
     1381 gc
//
LOCUS       DAOQDO010002057         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114233_length_1381_cov_2.541478, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002057 DAOQDO010000000
VERSION     DAOQDO010002057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114233_length_1381_cov_2.541478"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..732
                     /locus_tag="PKZ53_24930"
     CDS             205..732
                     /locus_tag="PKZ53_24930"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNJ43750.1"
                     /translation="MSAQTVANSLALAGPPSPPGNAMGTIGWIHIIFGSLALFFGLIV
                     VTLPKGTRLHRTVGHLYFSAMIGLNLTALSLYRLFKTVGPFHILALVSLGYVLTGVWY
                     AIRQTPRQTWLSKHIRFMSWSYVGLLAAATAEIACRIPGTNFWLAVLIPSCLVSVLGW
                     YIIENKAVVLKTQLP"
     gene            complement(811..>1381)
                     /locus_tag="PKZ53_24935"
     CDS             complement(811..>1381)
                     /locus_tag="PKZ53_24935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43751.1"
                     /translation="GLAGTLAEVYVGLNKPEKGLGYIEKALARDPFREDYWLTRGKLL
                     EANGQVEGAKSAYRRSIALAPELIESRFGLAQLAFRQQKYAEIESILHRDNELYPNLV
                     PSASALNLLAAAYQNQGKPLLALATMIEVVQKEDNPEYWITLARFQIALHRDPRESIN
                     RALACDPNQRQLLEADQVFAPYLEPKKLS"
BASE COUNT          319 a          365 c          323 g          374 t
ORIGIN      
        1 gagctgattt tggaattgaa cacctgattg tctcagaacc cgtccggttt gccaatcagc
       61 gggcaatcgg tcaccaacca ttcagttgcc aaattcaacc gttcagaacc agggatcgtc
      121 ctggtctaac ctgactgcgt acagtggtct gattttgtaa cgccacctca ttgaataact
      181 ggaaaggagc cggaggtgag ggcaatgtca gctcaaacgg tggccaattc tctggcactg
      241 gctggtcccc cctctccacc tggaaacgct atgggtacga ttggatggat tcacattatt
      301 tttgggagcc tggcactgtt ttttggcctg atagttgtca cactccccaa agggacacgc
      361 ctgcaccgga cagtaggcca cttgtacttt tcagccatga tcggattaaa tctcaccgcc
      421 ttgagcctgt accggttatt taagacagtt ggcccatttc atattctggc tctggtgagc
      481 cttgggtatg tgctgactgg tgtgtggtat gccattcgac aaacaccgcg tcaaacctgg
      541 ctctcaaaac atatccgatt tatgtcctgg tcatatgtgg gattactggc ggcagcaaca
      601 gccgagatag cctgccgaat tccgggcacc aatttctggc ttgccgtctt gattccatcc
      661 tgcctggtct ctgtccttgg atggtacatc attgaaaaca aagcggttgt tcttaaaacc
      721 caacttccgt gaacctgacc gctcatgttt ccccggtgat ccagacccgg caatttgaaa
      781 cttcaggccg ggttcagaat ccaggttgca ttaactgagt ttctttggtt ccaggtacgg
      841 ggcaaaaacc tgatcagctt ctagcaactg gcgctggttt ggatcacacg ccagtgcccg
      901 attgattgat tcacgtggat cccggtgaag tgcaatctga aatcgcgcca gggtaatcca
      961 atactccgga ttatcttctt tctgcaccac ctctatcatg gttgctaacg caaggagtgg
     1021 ttttccctgg ttttgatacg cagcagccag cagattcaac gcactggctg aaggcaccag
     1081 attaggatat aactcattgt ctctgtgtaa tatagattca atttccgcat atttctgttg
     1141 tcgaaaagct agttgagcca gaccaaaccg tgattcgatc aactctggtg ccagagctat
     1201 actgcggcgg tatgcagatt tcgcgccctc aacttgtcca ttcgcttcaa gcaactttcc
     1261 cctggtcaac cagtaatctt cacgaaaagg gtcgcgggcc agtgcttttt caatatagcc
     1321 aagccccttt tccggtttat tcaacccaac atacacttcg gctaaagtgc cagcaagccc
     1381 g
//
LOCUS       DAOQDO010002058         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114391_length_1380_cov_2.674717, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002058 DAOQDO010000000
VERSION     DAOQDO010002058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114391_length_1380_cov_2.674717"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1380
                     /locus_tag="PKZ53_24940"
     CDS             <1..>1380
                     /locus_tag="PKZ53_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43752.1"
                     /translation="VEPPGKTITAQGAIDISMRDIPDLDTSPNTRRGGFSIRLFKNGV
                     EYFPDPTRVKRELKKLWAEYRRRYPLQPEYEELFGFPKGQFRDLEQYYSIQNQYPNVY
                     GISEYGLPSAASEVRRGQAKQLKGYLLVFEQLLADYFSQLAQAKDLFSTRSDLDATYF
                     FQYLIKSVPNVKPLLGEEYFTGLPELIASQDPVTERRNRFLDFLLALFAERLDADSII
                     DSANSNQEAGTTGEQLLQAKLALLQHLVTSTQKRGSGFDYLAPPLPRNIAGMEIKCRI
                     QLGMEVYPERPLIDILEEQALELVETEAKATVGRSMSRHSDHISEHFTGVTAPTEAET
                     SSQMPPLLRAQSFTEEFLHAASEQTNFRTGILPGDTSTAVVCRSPNEKNWRLVGKYPD
                     PETAQASVPALVNLVQKLRRFSQQLYIIEHTLLRFGRADRRPPPASTSEELQRESPRD
                     PQPFHYSFTI"
BASE COUNT          334 a          400 c          343 g          303 t
ORIGIN      
        1 gttgaaccac ctgggaaaac gatcacagct cagggggcga ttgatatttc aatgcgggat
       61 atccccgacc ttgacacctc gcccaatacc cggcgtggag gattttcaat ccgattattc
      121 aaaaatggtg ttgagtattt tcctgatcca acgcgggtta aacgcgaatt gaaaaaactc
      181 tgggccgagt accgccggag gtatccgtta cagcctgagt atgaagagtt gtttggcttc
      241 cccaaagggc aatttcgtga cctggagcag tattattcga tccagaacca gtatccgaat
      301 gtctatggca tcagcgaata tggactgcct tcggcggctt cggaggttcg gcgcggacag
      361 gccaaacagc tcaagggcta cctgctggtt tttgaacaac tgctggcgga ttacttctcg
      421 caactggctc aagccaaaga ccttttttca acccgttcgg atctggatgc cacctatttt
      481 tttcagtacc tgatcaaatc ggtccccaac gtcaaaccgc tgctgggcga agaatacttc
      541 accggattgc ctgaattgat tgcgagccag gatccggtga ccgaacgccg caaccggttt
      601 cttgatttcc tgctggccct ttttgccgaa cgacttgatg cggattccat cattgattcg
      661 gccaactcaa accaggaagc tggcaccact ggcgaacaat tgctccaggc caaactggcg
      721 ttgctccagc atctggtcac cagcacccaa aagcgtggaa gcgggtttga ttacctggcg
      781 ccgccgttgc cgcgcaacat tgccgggatg gaaatcaaat gccggattca gcttggaatg
      841 gaggtttatc ccgaacgccc attgattgac attctcgaag aacaggccct ggaactggtt
      901 gaaaccgagg ccaaagccac cgttggccgg agcatgagcc gccattccga tcatatttcc
      961 gaacacttta ccggtgtcac cgccccaacc gaagccgaga catcttccca gatgccgccg
     1021 ttgctgcgcg cccaatcgtt tactgaagaa ttcctgcacg ctgccagcga gcagaccaac
     1081 tttcgaactg gcatcttgcc cggagatact tcaaccgctg tggtgtgccg gtctccgaat
     1141 gaaaaaaact ggcgactggt cgggaaatac cctgacccgg agaccgccca ggccagcgta
     1201 ccggccctgg tcaatctggt acaaaaactg cggcgattta gccagcaact gtacatcatc
     1261 gaacatacct tgctgcgctt tggtcgcgcc gaccgaagac cgcccccagc ttcaaccagc
     1321 gaagaactcc aacgtgaaag cccacgagac ccacagccat ttcactacag tttcacgatc
//
LOCUS       DAOQDO010002059         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114517_length_1379_cov_4.504532, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002059 DAOQDO010000000
VERSION     DAOQDO010002059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114517_length_1379_cov_4.504532"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1186
                     /gene="ilvC"
                     /locus_tag="PKZ53_24945"
     CDS             152..1186
                     /gene="ilvC"
                     /locus_tag="PKZ53_24945"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNJ43753.1"
                     /translation="MKVYYDADADLSLLKGKKFAILGYGSQGHAHAQNLRDSGFDVIV
                     GLRRGSASQPKAESAGFKVFDTAEAARQADITMILTPDELQPEIYRQELSPVFEAAAA
                     EGKPKYIAFGHGFSIHFKKIVPPAATNVFMVAPKSPGHLVRKEYANGGGVPALIAVHQ
                     NPAGNTREIALAYATAIGSGKAGILETTFREEVETDLFGEQAVLCGGLTELIRAGFET
                     LVEAGYSPEMAYFECLHEMKLIVDLVYEGGIANMRYSISNTAEYGDMTRGKRIITQDT
                     RSAMKELLTEIQSGHFADEWIAEHNAGKPNYKRMASDQANHQIEQVGAKLRSMMPWIE
                     RNRLVNKAKN"
BASE COUNT          359 a          311 c          396 g          313 t
ORIGIN      
        1 tgcgtgagcg ggtgggtcaa agagtcagta ccacctgcgt gagcgggtgg gtcaaagagt
       61 cagtaccacc tgcgtgagcg ggtgggtcaa agagtcagta ccacctgcgt gagcgggtgg
      121 gtcaagaata aaaaacgttg ggaggatgaa aatgaaggtt tattatgatg cagatgcgga
      181 tttgtcttta ttaaaaggaa aaaagtttgc cattttgggt tacggcagcc agggacacgc
      241 ccacgcgcag aatttgcgcg atagcgggtt tgatgtgatt gttgggcttc gccggggcag
      301 tgcatcgcaa cccaaagccg aatcggctgg attcaaggtt tttgacaccg ccgaagccgc
      361 ccgccaggcc gacatcacca tgattcttac gccagatgaa ctgcagccag aaatttatcg
      421 ccaggaacta tctccggtat ttgaggcggc ggcggctgag ggaaaaccca aatacattgc
      481 atttggacac ggattttcaa ttcattttaa gaaaatcgta ccgccagcgg cgaccaatgt
      541 gtttatggtg gcgccgaaat cgccagggca tcttgtccgc aaggaatatg ccaatggcgg
      601 cggcgttccg gcactgattg cggtccatca aaacccagcc ggaaacaccc gcgaaattgc
      661 gttggcctat gccacggcga ttggttcggg gaaagctgga attttggaaa cgaccttccg
      721 cgaagaagtg gaaaccgatc tttttggtga acaggccgtt ctgtgcggcg ggttgacgga
      781 actgatccgg gccgggtttg aaacactggt cgaagccgga tattcgccgg aaatggccta
      841 ttttgaatgt ttgcacgaaa tgaagctgat tgtggatctg gtgtatgaag gcggcattgc
      901 caatatgcgc tattcgatca gcaatactgc tgaatatggc gacatgacac gtggaaaacg
      961 aatcatcacc caggataccc gttccgccat gaaagagttg ctgaccgaaa tccaatcagg
     1021 acattttgct gatgaatgga ttgccgaaca caacgccggc aaacccaact acaagcgcat
     1081 ggcgtccgat caggccaacc atcaaattga acaggtcggt gccaaactcc ggtcaatgat
     1141 gccgtggatt gagcgcaacc ggctggtgaa caaggcgaag aattagggct tggggcggaa
     1201 gaaatcgggc ggaagacgtc gggctttggg ctgaagactc gcaagctcgg ggctgaagac
     1261 gatgatttca gccccaaact ctaaaatgtt cgaggtcgaa caattcttgt aatctattga
     1321 tttgaagata tttaacctcg attagtattt tccttcaggc tatgatattt tctgctttc
//
LOCUS       DAOQDO010002060         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114703_length_1378_cov_3.571429, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002060 DAOQDO010000000
VERSION     DAOQDO010002060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114703_length_1378_cov_3.571429"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..548
                     /locus_tag="PKZ53_24950"
     CDS             123..548
                     /locus_tag="PKZ53_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43754.1"
                     /translation="MPTGPLKRLCFEADTGLHPEFHGCGQVLRVLWNGIYVGRVLQSR
                     ESATWGNDQELGTYPWKTPEEASFHLIGRNEVTRLALQENYEWNVYLADTGGFWMGPF
                     PSREEAERAVHTTAGFDLPFASQIRQDPVGTAKNYLTSK"
     gene            931..>1378
                     /locus_tag="PKZ53_24955"
     CDS             931..>1378
                     /locus_tag="PKZ53_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNJ43755.1"
                     /translation="MTKFKVLVCGNLAEDGLAVFRAADNVELDVKPELKEDELVACIG
                     PYHGLVVRSDTKPTAKVIAAAENLKVIGRAGTGVDNIDVKAATKRGVVVMNTPGGNTV
                     TTAEHSFALLMALARNIPQGTASLKAGRWDRKKLMGVELCNKTIGIL"
BASE COUNT          344 a          316 c          343 g          375 t
ORIGIN      
        1 ggcgtattgt tgagtctttt cctggctgtg gtctttacca ttgtgtacac cattttcctg
       61 ggcttatggt tttggttttt tggtagtttg ccgttttgat ttcctgaaag gagcttgaat
      121 ctgtgccgac gggaccactc aaacgacttt gttttgaagc tgatacaggt cttcaccccg
      181 aattccacgg ctgcgggcag gtactgcggg ttttgtggaa tggtatttat gtgggccggg
      241 tccttcaaag ccgtgaaagt gccacctggg gcaatgacca ggagcttggg acctatccct
      301 ggaaaacacc tgaagaagca tcctttcatt tgattggacg caatgaggtc acgcgactgg
      361 cgcttcagga aaactatgaa tggaatgtct atctggctga tacaggcggg ttttggatgg
      421 gcccctttcc ttcgcgcgaa gaagccgaac gtgctgtcca caccactgcc gggtttgacc
      481 tcccctttgc cagccagatt cgccaggatc cggttggaac agccaaaaac tatctgacct
      541 caaaatgaag cattgagaat caagaatgaa gaatgaagaa ttaagaatta agaattaaga
      601 atcaagaatg gagaatgaag aataagtcca gtcagtcgtt tagcgtgtag attggaaaat
      661 gcctgtcaga ggggtcttgt tcgaaaagtc aacttgatga aggctctttt aaagaaataa
      721 caaattagat attaccggag ttagggcctc gcctcaggtc gactattgat tgaaagcact
      781 tacaatcgcg atttctttat caccaataat gcctattctt cattctcaat tcttcattct
      841 tcattcttca ttctcaactc ttcattctca attcttcatt ctcaattctt catccccctt
      901 atactctcca caaaggtaat ttttttgtat atgacgaagt ttaaagttct tgtctgcggt
      961 aacttagccg aggatggcct ggctgtgttc cgggctgcgg acaatgtcga actggacgta
     1021 aagccggaat tgaaagaaga cgaactggtc gcctgcatcg gcccctatca cgggctggtc
     1081 gttcggagcg ataccaaacc aaccgccaaa gtgattgcgg cggctgaaaa cctgaaagtc
     1141 attggccggg ctggcactgg cgttgacaac attgatgtca aggctgctac caagcgtggg
     1201 gtagtcgtga tgaatacccc tggcggtaac accgtgacca cggctgaaca cagttttgcc
     1261 ctgctgatgg cacttgcccg caacattccc cagggtacgg cttcgctcaa ggctggccgc
     1321 tgggaccgaa aaaaactgat gggtgttgaa ttgtgcaaca aaaccatcgg gattcttg
//
LOCUS       DAOQDO010002061         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114718_length_1378_cov_3.074830, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002061 DAOQDO010000000
VERSION     DAOQDO010002061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114718_length_1378_cov_3.074830"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKZ53_24960"
     CDS             complement(<1..655)
                     /locus_tag="PKZ53_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ43756.1"
                     /translation="MSAKPEQTNSWQPEIDELRKREELAQEMGGAEKVAKHRSQGKLT
                     VRERIDAVLDIETFHEKGVLAGTSKYDQNGKLTSFIPANFVCGTGNINGRKVVVGGDD
                     FTVRGGAADAAIIAKQIYAEQMASSLQIPIVRLVDGTGGGGSVKLLETHGFTYVPFNP
                     GWDYVVDNMSVVPVVAACLGSVAGLGAARVVASHFSVMTEHTAQLMVAGPPVVKFGTG
                     "
     gene            complement(854..>1378)
                     /locus_tag="PKZ53_24965"
     CDS             complement(854..>1378)
                     /locus_tag="PKZ53_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017712008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ43757.1"
                     /translation="WYPVEGNPKIRQAPDAMVVFGRPKGHRGSYLQWKEENIAPQVVF
                     EILSPGNTYGEMVRKLEFYERYGVEEYYVYDPDHNELNGWQRQEGRFQTIAAIDDWTS
                     PRLKIRFERTPESLKLYRPDGTLFETFAELLVRAEADRQRAETLQREKDQTQAKLERL
                     AARLRELGEDPDLP"
BASE COUNT          292 a          370 c          344 g          372 t
ORIGIN      
        1 cgccagttcc gaatttgacg accggcggcc ccgcgaccat caactgggcg gtgtgttcgg
       61 tcattaccga aaaatgcgat gccacgacgc gagccgcacc caggcccgcc accgaaccca
      121 gacaggcggc aaccaccggg accacactca tgttgtcaac cacataatcc caccctggat
      181 tgaacggcac ataggtaaat ccgtgcgttt caagcagttt gacgctgccg ccgccgccag
      241 tcccatccac caggcgcacg attggaatct gcagactgct ggccatttgt tcagcataga
      301 tttgtttggc aatgatggca gcatcggcgg cgcctccgcg aaccgtgaaa tcatcgccgc
      361 cgaccacaac ctttcggcca ttgatgtttc cggtaccaca gacaaaattt gccgggataa
      421 agctggttaa cttcccattc tggtcatatt tggaagtccc agccaggacg cctttttcgt
      481 gaaatgtctc aatatcgagg acggcgtcaa tccgttcgcg aacagtgagt ttgccttgtg
      541 aacgatgttt ggcgactttt tcggcaccac ccatctcctg ggcgagttct tcgcgtttgc
      601 ggagttcatc aatttctggt tgccaactgt tggtttgttc tggttttgcg ctcattgtgg
      661 gattcagggt tcagggttca gggttcaggg ttctttgtac ttcgttcttg gttctttgtg
      721 cctggttctt ggttcttggt tcttggttct tggttcttgg ttctttgtgc ctgattctta
      781 gtgcctggtt ctttaaagaa ttggttccaa agtaccaagc accaagtacc aagtaccaat
      841 caccaagcac aacctatggc agatctggat cttcgccaag ctcgcgcaac cgggcggcca
      901 gtcgttctaa ctttgcctgg gtttgatcct tttcacgttg caaagtttcc gcacgttggc
      961 ggtcagcctc agcacggaca agcaattcag caaaggtttc aaacaacgtg ccgtctggcc
     1021 gatacaattt cagagattcg ggagtgcgtt caaagcgaat tttcaaccgg ggactggtcc
     1081 aatcgtcaat tgctgcaatt gtctggaagc gtccttcctg gcgttgccag ccgttgagtt
     1141 cgttatggtc agggtcatag acgtaatatt cctcgactcc ataccgctca taaaactcca
     1201 gcttgcggac catttcacca taagtatttc ctggtgaaag gatttcaaaa acgacttgtg
     1261 gagcgatatt ttcttctttc cactggagat atgacccgcg atgacctttg ggacggccaa
     1321 aaacaaccat ggcatcgggg gcctgacgga tttttgggtt tccttcgacc ggatacca
//
LOCUS       DAOQDO010002062         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114867_length_1377_cov_3.531014, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002062 DAOQDO010000000
VERSION     DAOQDO010002062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114867_length_1377_cov_3.531014"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="PKZ53_24970"
     CDS             complement(<1..1298)
                     /locus_tag="PKZ53_24970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ43758.1"
                     /translation="MQQVNEQLRSREQHFRTLTTLAPVGIFLNNAEGECIFVNEAWSE
                     ITRLPVEEGLGQKWFQIIHPDDREYVHTQWQRCRVLGEEVSFEMRILRLDATIAWISG
                     RVRPVKSRPGEVIGYVGTVMDITESRQLEEMLHEREATLRALGDNLPNGAIYQIKHDR
                     DGKPQFNYFSAGIEHIIGIPREVIFADPSVLMQQLSEADQIRFNQAVEKAVQTQTLID
                     EQVLKQTPHRGPVWTQIRTSPRRLADGTVVWDGIEFDITESKQLEAELRRSKDLFQAV
                     FEESADALFLVDAKTILITDCNQRVVEMFEGESKTDFIGTYGEHLQKYRFTPDELASL
                     GERARSGDFVSLELEYVTKKGTEFWGNLAFKLIDVGGSRLQLVRVTDITTQKQAETAL
                     INSEHRFRTIFDTAFQMSGLLTPEGVVLQANQAALDFARLT"
BASE COUNT          317 a          357 c          334 g          369 t
ORIGIN      
        1 gtgagtctgg caaaatcgag ggcggcttga ttggcctgga gaacaacccc ctcaggtgtc
       61 agcaatccag acatttgaaa ggcggtgtca aatatcgttc gaaaccgatg ttcactgttg
      121 atcaatgccg tttcagcctg tttttgggtc gtaatatcag tgacccggac cagttgcaat
      181 cggcttccac ccacatcaat gagtttaaag gccagattcc cccaaaattc agttcctttt
      241 ttggtgacat attcgagttc aaggctgaca aaatctcctg agcgagcccg ttccccgagt
      301 gatgccagtt cgtcgggggt aaaccggtat ttttggaggt gttcgccata cgtgccgatg
      361 aaatcagttt tggattcgcc ttcaaacatt tccacgacgc gctggttgca atcggtaatc
      421 agaattgtct tagcatcaac cagaaacaga gcatcggctg attcttcaaa gactgcctga
      481 aacaggtctt tgcttcggcg tagttcggcc tccagttgct tgctctcagt aatgtcaaat
      541 tcgatgccat cccagacgac agttccatcc gcaagcctgc gtggtgaggt ccgaatctgc
      601 gtccacaccg gcccccgatg gggcgtttgc ttgagtacct gttcatcaat cagggtttgt
      661 gtctggactg ccttttcaac tgcctgatta aacctgatct gatcggcttc gctcagttgc
      721 tgcatcagca ctgaaggatc agcaaaaatc acttcccgag ggatcccaat aatgtgttcg
      781 atccccgcac taaaatagtt gaactgtggt ttgccatccc ggtcgtgttt aatctggtaa
      841 atggcaccgt ttggcaggtt atcccctaag gcccgcaagg ttgcttctcg ttcgtggagc
      901 atttcctcaa gctggcgaga ttctgtgatg tccatcacgg ttccgacata gccgataact
      961 tctccgggtc tacttttgac gggtcgcacc cgacctgaaa tccaggcaat ggttgcgtcc
     1021 agacgcagga ttcgcatctc aaacgaaact tcttcaccca ggacccggca gcgctgccac
     1081 tgagtatgga catattcccg atcatcagga tgaatgattt gaaaccattt ttggccaagt
     1141 ccttcttcaa ccggcaaccg ggtgatttcc gaccaggctt cgttgacaaa gatacattcg
     1201 ccttcagcat tattgaggaa gattccaacc ggggccaggg tcgtcagggt gcgaaaatgt
     1261 tgctcacgtg agcggagttg ctcattaact tgttgcattt gaagttgatg tttcaattgc
     1321 aggtttgacg cccacatcat aacgcccgtc attataacga tggtgacagc cgtcatc
//
LOCUS       DAOQDO010002063         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114890_length_1377_cov_2.835855, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002063 DAOQDO010000000
VERSION     DAOQDO010002063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114890_length_1377_cov_2.835855"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..479)
                     /locus_tag="PKZ53_24975"
     CDS             complement(156..479)
                     /locus_tag="PKZ53_24975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43759.1"
                     /translation="MSLSKISRKRQVVIPEDICRAIGAHIGDFVEFVQRENDVLLKVK
                     KVVDADLVPWGKPGGLPPAPSYEERMRMLKELEGDAEDDTYDIPIEEIRASRTRKELP
                     VSLDE"
     gene            complement(813..>1377)
                     /locus_tag="PKZ53_24980"
     CDS             complement(813..>1377)
                     /locus_tag="PKZ53_24980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43760.1"
                     /translation="AEFLSIRSSLFSELDAAGSTVKTVEFEYVTPRRYSRNTVVIAPF
                     IKTTAGVFLAVQHRDLPAAQYFTGNSMLVVAPGWRVPFEVTTQWELPTFIEANLKREF
                     GVTARMIQELGGPYFPTPGATPEIVYPFAVELAAQSLEASSLHLVGINEFLNQLESIE
                     DAHLLIVGLRLAHALGFLLAAPDLHQA"
BASE COUNT          374 a          317 c          313 g          373 t
ORIGIN      
        1 atgttttggg tttcaactcg aatgattgag ggagtttaaa ttacaaagta ttcgatggca
       61 agtctttttt tatcaaccat aaagaatttg ttcctgatat aagtggttgt agttggtaaa
      121 ggatgagtat aaaaaataaa actgaattca atacgttact cgtcaagaga tactggcaat
      181 tcctttctgg tgcgtgacgc cctgatttct tcaatcggaa tatcataggt gtcatcttcg
      241 gcatcacctt ctagctcttt gagcatcctc attcgttcct cataggacgg agccggaggc
      301 agcccccctg gcttacccca aggaacaagg tcagcatcca ctaccttttt gaccttgaga
      361 agtacatcat tttctctctg tacaaattct acaaaatcgc caatatgcgc gccgattgcc
      421 cggcaaatat cttcaggaat caccacctgt cttttgcggc tgatcttgct taatgacata
      481 gtgccccctg tgtctttcct ttcaaacgtc atacagtttg atggttaaat tgtaaaacgg
      541 taaaacccat agtcattgta ggtcaaaaaa agccgaacac aagcaaattg aacttgttaa
      601 accaggtagg tgccatagta tgaaggcttt ggcgatgaaa agaacaagaa cggttgccgt
      661 gacttgcgcc atcatcgttt tttcatcagc accttgattt ggttcagaaa tcgtaactga
      721 ttttcatcgg tcagacattg aaaccgttct atcaatattt gcagggtttg gtcaaactgg
      781 gttgcagtta gttcagggta aatgacctgg acttatgcct gatgcagatc tggagcagcc
      841 agcaaaaacc ccaaggcgtg cgccagtcgc agcccgacaa tcaacaagtg ggcgtcttcg
      901 attgattcaa gctggttcaa gaattcattg attccgacca gatgcaatga acttgcttca
      961 agcgattggg ccgcaagctc caccgcaaat ggatacacaa tctctggcgt ggcgccgggc
     1021 gttggaaaat acggcccgcc aagctcctga atcattctgg cggttacacc aaattcacgc
     1081 ttcaaattgg cttcgataaa agttgggagt tcccactggg ttgtgacctc aaatggaact
     1141 cgccagccgg gtgccacgac gagcattgaa tttccggtaa aatattgggc tgcgggaaga
     1201 tcccggtgct gaactgccag aaatactccg gcggtggttt tgataaatgg cgcaatcacc
     1261 accgtgttgc ggctgtagcg ccgtggcgtc acatattcaa attcaacggt ttttactgtc
     1321 gaacctgcag catcaagttc tgaaaaaagg ctgcttcgaa tcgaaagaaa ctcagcc
//
LOCUS       DAOQDO010002064         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_114935_length_1377_cov_2.188351, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002064 DAOQDO010000000
VERSION     DAOQDO010002064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114935_length_1377_cov_2.188351"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1377
                     /locus_tag="PKZ53_24985"
     CDS             <1..>1377
                     /locus_tag="PKZ53_24985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ43761.1"
                     /translation="IALEQHVEQFDLTFVMAEVAGCLTGAFQYNTSLFEESTIARLKG
                     HFETLLTDLMDRPHVQVSHLNLLSEPEQVWLTQWNQTQAAYPDEACLHALFEHQARLH
                     PDGLAVESDDEQLTYGELNRQANQIAHRLQAMGVQTDMPVGVCLNRSAKLVAVLLGIL
                     KAGGGYVPLDPGYPKERLGFMLTDCRTPVLVTESSLLAQLPEYDGHTLLLDVEWKALK
                     FEDDQNLPATGVSSNLAYVIYTSGSTGKPKGVSCNHRGVVNLLTDIDRRQPLSPEDGC
                     SWWTSLSFDVSVYEIFSALLFGATLHIPTEEVRLNSPAFLGWLAAHGIRSAYIPPFML
                     REFEARLQNGAADVQLRRLLVGVEPIPESLLASINRQLPDLRIINGYGPTEASICATL
                     HTFDPLQAREHKNVPIGRPVQNTVIYVLDTNLQPVPVGVAGELYIGGDGLARGYLHHG
                     GLTVEKFI"
BASE COUNT          296 a          379 c          376 g          326 t
ORIGIN      
        1 ccatcgcgct tgaacaacac gtcgaacaat ttgacctgac gtttgtcatg gccgaagtcg
       61 ctggttgttt aaccggagcg ttccagtaca acaccagcct ctttgaagaa agcacgattg
      121 cccggctcaa agggcatttt gaaaccttgc tgactgacct gatggaccgg ccacacgttc
      181 aggtgtcaca cctcaatctg ctatcggagc cggaacaggt ctggctgacc cagtggaacc
      241 agacccaggc tgcctatcca gatgaagcct gtctccatgc tttgtttgaa caccaggcgc
      301 gccttcatcc agatggattg gcagtggaaa gtgatgacga acagcttacc tatggtgaac
      361 tgaaccggca ggcaaatcag attgcccatc ggttacaggc catgggggtt caaacggata
      421 tgccggttgg tgtgtgcctg aaccggtcag ccaaactggt ggccgtcttg cttgggattt
      481 tgaaagccgg cggtggctat gttccgctgg atcctggcta tcccaaagaa cgacttggat
      541 ttatgctcac cgactgccgg acgccggtgc tggtgaccga atcttcgctg ctggcacaac
      601 ttcccgaata tgatggccat accctgttgc ttgatgttga gtggaaagcg ctgaagtttg
      661 aagatgacca gaatttgcct gcgacgggtg tttcaagcaa tctggcctat gtgatttaca
      721 cctccggttc aaccgggaaa cccaaaggcg tcagttgcaa ccaccggggt gtcgtcaatc
      781 tgctcaccga cattgaccgt cggcagccgc tgtcacccga agacggctgt agctggtgga
      841 ccagccttag ttttgatgtc tcagtctatg aaattttttc cgcgctcctg tttggtgcca
      901 cactccacat tccaaccgag gaagtccgct taaacagccc tgcgtttttg ggctggctgg
      961 cggcgcacgg catccgaagc gcctacattc cgccgtttat gctgcgcgaa tttgaagccc
     1021 ggcttcaaaa cggagcggca gacgttcaat tgcgccgatt gctggtgggg gtggaaccga
     1081 ttcccgaaag cctgctggcc tcaatcaatc gtcaacttcc tgacctgcgc atcatcaacg
     1141 gctatggtcc gaccgaagcc agtatttgtg ccacgctgca cacctttgat ccgcttcagg
     1201 cccgcgaaca taaaaacgtg cccattggcc gtccggtaca aaacacggtt atctatgtac
     1261 tcgataccaa tttgcagccg gtgccggttg gcgtggctgg tgaactctac atcggcggtg
     1321 atggtctggc gcgcgggtat ctgcatcacg gtgggctgac ggttgagaaa tttatcc
//
LOCUS       DAOQDO010002065         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_115120_length_1376_cov_2.032551, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002065 DAOQDO010000000
VERSION     DAOQDO010002065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115120_length_1376_cov_2.032551"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKZ53_24990"
     CDS             complement(<1..222)
                     /locus_tag="PKZ53_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNJ43762.1"
                     /translation="MEIAILLFDRFTALDAVGPYEVLRSIPGATVKFVAKQPGPVRTD
                     ARSLAITADFALDEITAPDILLIPGGPGQT"
     gene            complement(291..1232)
                     /locus_tag="PKZ53_24995"
     CDS             complement(291..1232)
                     /locus_tag="PKZ53_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43763.1"
                     /translation="MGFFRKLFGLGSTVPEWASFFSLEEYQRFNQLIEQHFVATQTKI
                     SLQDGIVVVVATKQKYGLQNLAQVCCQSEAESWTEVIANHFETIQQAEAEGREIQEKF
                     SDFSKIAPYLAVRLYPDSYLEQVGADKAVASIDLEGTVTMLVLDLENAIRTVCTDDIK
                     PWGKSNYELIRLGLNYVRQINDVEIEDLELTEDVSVKIMSGDSFFASTQILMLEDFSP
                     CLGLYGSLVGVPHRHTAIIYPIQNLDVIKAVNVLIPLIKGLYNEGPGSISPNLYWYRH
                     QKFITLPYRETRKALQFYPPAEFTDMLNRMAASDTSQ"
BASE COUNT          389 a          347 c          313 g          327 t
ORIGIN      
        1 cgtttgccca gggcctccgg ggatgagcag gatgtctggc gcagttattt cgtcgagtgc
       61 aaaatctgcc gtaattgcca gacttctggc atcagtccga actggaccgg gctgtttggc
      121 cacaaatttc acggtggccc caggaattga tctcaacact tcatacgggc caacggcatc
      181 cagggcggta aagcggtcaa agagaagaat tgcgatttcc aaacgacacc tcatttcatc
      241 gaaattgtcc aacaataatc aaggaacctt agcggatgac gaactacggg ctactgactg
      301 gtatcagaag cagccatccg gttcagcata tcggtaaatt cagccggagg ataaaattgc
      361 agagctttgc gcgtttcacg gtagggcagc gtaatgaatt tctggtgccg gtaccagtac
      421 aaattcggac tgatggagcc ggggccttca ttgtataaac ctttgatcaa cgggattaga
      481 acgttgacgg ctttaattac atcgaggttt tgaatggggt agataatggc cgtatgacga
      541 tgtggcacac caacaagcga accatacaac ccaagacacg gcgagaagtc ttcgagcatc
      601 agaatctggg tcgaagcaaa gaagctatca cccgacataa ttttgaccga aacatcctca
      661 gttaattcaa gatcctcaat ttcaacatca ttgatctgac ggacataatt caagcccagt
      721 cggatcagtt catagttgga tttcccccag ggtttgatat catcagtaca gaccgtccga
      781 atcgcgtttt ccaaatccag aacaagcatg gtcacggtgc cttccagatc aatcgaagcc
      841 accgctttgt cggcaccaac ctgttccaga taactgtcag ggtacaatcg aactgccagg
      901 taaggagcga ttttggaaaa atcagaaaac ttttcctgga tttcgcggcc ttcagcttca
      961 gcttgttgaa tggtttcaaa atgattggca atcacttcgg tccaggattc agcttcagac
     1021 tggcaacaaa cctgagccag gttttggagg ccatatttct gtttggtagc caccaccacc
     1081 acaatcccat cctgcaatga gattttagtc tgagtcgcaa caaagtgttg ctcaatcaac
     1141 tggttaaagc gctggtattc ctcaagcgag aaaaacgacg cccactcagg aactgtggaa
     1201 ccgagcccaa atagttttct gaaaaacccc atgccgtgcc tcgtttcgac ccgtaacttt
     1261 tcttgaaagc aagtacctgc tgaaaattcc aggccatctt aaaccacgga aaccacgaaa
     1321 aacacgaaaa aagtcaacca cttctttcat caccgtgtca cggcgccgtt cccatc
//
LOCUS       DAOQDO010002066         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_115229_length_1375_cov_2.698485, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002066 DAOQDO010000000
VERSION     DAOQDO010002066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115229_length_1375_cov_2.698485"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..396)
                     /locus_tag="PKZ53_25000"
     CDS             complement(55..396)
                     /locus_tag="PKZ53_25000"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNJ43764.1"
                     /translation="MKFDSVLFFLTIFWLVVTNLDVTAAVIPQTPSLRPLLSKAVKAN
                     KTVRFAIEGMHCKGCVRAIETDVSSIVGVKSCKVNLEKKRAEVTFDPIAVSEQIIIDR
                     IKKLGYTARVA"
     gene            589..1371
                     /locus_tag="PKZ53_25005"
     CDS             589..1371
                     /locus_tag="PKZ53_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNJ43765.1"
                     /translation="MSLRIEKNGPLTTVILDRREVRNAVDRPTAQALADAFREFEADP
                     TALVGVLCGDHGTFCAGADLKTIATGNGNRLEPTGDGPMGPSRLILSKPVIAAISGYA
                     VAGGLELALWCDLRVMEADAVLGVFCRRWGVPLIDGGTVRLPRLIGLSHALDLILTGR
                     PVLAEEALRLGLVNRVVPTGKAREAAEELAWQLAAFPQTCLRADRISAYESFDRPFEA
                     AVAFEFENARPVLQTETQAGAQRFADGAGRHGAFDPNAPTKS"
BASE COUNT          321 a          356 c          379 g          319 t
ORIGIN      
        1 ggaacccgga acccggaacc cggaacccgg aacccggaac ccggaacccg gttctcaagc
       61 cactcgtgcc gtgtagccca gcttcttgat gcggtcaatg atgatctgtt cagaaacagc
      121 aattgggtca aaagtgactt cggcgcgttt cttttccagg ttgaccttgc aactttttac
      181 ccccacgatt gaggacacgt cagtttcaat tgctcgaaca catcccttac agtgcattcc
      241 ttcaattgca aaccgaaccg ttttgttggc ctttaccgct ttgctcagga gtggtctcag
      301 cgaaggtgtt tgaggaatca ccgctgcagt cacatccaga ttcgtcacaa ccagccagaa
      361 gatcgtcaag aaaaacaata ctgagtcaaa tttcataaaa accttttcaa atggaaaaat
      421 gatttgggat tggaggaaac gagactgtac cacatttaaa gcgaggagtg agtagtgagg
      481 agcgaggagt cagattaagg aaaatagtga agctggtatc actgagtcgc tcttcaatca
      541 attaccggca ggatgccggc gctctcagga actatcaagg agaaaccaat gtcgcttcga
      601 attgaaaaaa atggaccgct gacgactgtg attttggatc gccgcgaagt ccgcaatgca
      661 gttgaccgcc cgacggctca ggccctggct gatgcgttcc gtgaatttga agctgatccg
      721 acagcactgg tcggtgtctt atgcggcgac cacgggacgt tttgcgccgg tgctgattta
      781 aaaaccattg ccactggaaa cggaaatcgt cttgagccga ctggcgatgg cccgatggga
      841 ccatcacgcc tgattctttc caaacctgtg attgctgcca tttcggggta tgccgtggct
      901 ggcggactcg aactggcctt gtggtgcgac cttcgcgtga tggaagctga tgccgtgctg
      961 ggtgtgtttt gtcggcggtg gggagttcct ctcattgatg gaggcactgt ccggctgccc
     1021 cgactgattg gtttgagcca cgccctggat ttaattctga ccggacggcc cgttctggct
     1081 gaagaagcac tgcgcctggg cctggtcaat cgggttgttc cgaccggtaa agctcgcgaa
     1141 gccgccgaag aactcgcctg gcaactcgca gcctttccac aaacctgcct gcgggcagat
     1201 cggatatccg cttatgaaag ctttgaccgg ccatttgaag ctgccgtggc ttttgaattt
     1261 gagaatgcca gaccggtgtt gcagactgaa acccaggccg gcgcccagcg ctttgccgac
     1321 ggtgctggac ggcatggggc atttgatccg aacgcaccaa caaagtcttg aaaca
//
LOCUS       DAOQDO010002067         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_115530_length_1373_cov_3.490895, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002067 DAOQDO010000000
VERSION     DAOQDO010002067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115530_length_1373_cov_3.490895"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1373
                     /locus_tag="PKZ53_25010"
     CDS             <1..>1373
                     /locus_tag="PKZ53_25010"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNJ43766.1"
                     /translation="SQNIGSAQLFRLEEYKLPEFKVSVTTPEENGRKKAFKLGETVEV
                     AVQADYYFGGPVANANVQVVVYQNPYYQSWQPPRDYPWFYQEADQRGYRGYGNGQVIK
                     QETLKTDLNGKAVLTFDTPQTGQVDFEYRVEARVTDASRREIIGSDSVRVTRQRYYVY
                     LQPDHTIYHPQDRVKIKVKALDANRNPVQTDGTILVSRETWHEIWLDPTGREVTGDEL
                     KQLQASPKGFPPQLKPGECGWQMKSRGYKREEVLKQIVKTDAEGNGEFNFVAAQEGYY
                     SANWTSVDQDQRPIFGSTAFWVSTTGNADLGFRPTEVKIVVDQDTAVVGQTIPVLITS
                     PYTDRYVLFSVEGEELYSHQLVHLTSNTKLVEVPIDSRHIPNVSFRAVMVSNYQVMGD
                     SVEVIVPPTRNFLSVEVKADREEYQSRQDGQLTITTRDASNKPVSAEVSLGLVDESVF
                     YIQQEYA"
BASE COUNT          360 a          354 c          360 g          299 t
ORIGIN      
        1 ggtcgcaaaa tatcggctcg gctcaactgt ttcgacttga agagtacaaa ctgccggaat
       61 tcaaagtcag cgtcaccaca cctgaagaaa atggccggaa aaaagccttc aaactgggtg
      121 aaacggtcga agttgcggtc caggctgact attactttgg tggtccagtc gccaatgcca
      181 acgtgcaggt ggtggtgtat caaaatccgt attatcaatc ctggcaacca ccgcgtgact
      241 acccgtggtt ttaccaggaa gccgaccagc gcgggtatcg cggttacggc aatgggcagg
      301 tcatcaagca ggaaaccctc aaaactgatc tcaatggcaa agccgtgctg acctttgaca
      361 caccacaaac ggggcaggtg gattttgaat atcgcgttga agcccgtgtg accgatgctt
      421 cgcggcgcga aatcatcggg agtgattcag ttcgcgtcac ccgccagcgg tattatgttt
      481 atttgcagcc agatcacacc atttaccatc cacaagatcg agtcaaaatc aaagtcaaag
      541 cgctggacgc caaccgcaat ccagttcaaa ccgacggcac catcctggtt tcgcgtgaaa
      601 cgtggcacga aatctggctt gatccgacag gccgcgaagt caccggcgac gaattaaagc
      661 agttgcaggc gtcgccaaaa ggatttccgc cacaactcaa gccaggcgaa tgtggctggc
      721 agatgaagtc acggggctac aaacgcgaag aagtcctcaa gcaaattgtc aaaaccgatg
      781 cggaaggcaa cggggaattt aatttcgtcg cagcccagga aggctactat tccgccaatt
      841 ggaccagcgt tgatcaggat cagcgaccaa ttttcgggtc aacagctttc tgggtctcga
      901 cgacgggaaa tgctgacctt ggctttcgcc caactgaagt caaaattgtg gttgatcaag
      961 acacggcggt tgtcggccag accataccgg tgttgatcac gtctccatac acggaccgct
     1021 atgtcctttt ttcagtggaa ggtgaagaac tctacagcca tcaactggtc catttaacct
     1081 ctaacaccaa actggtcgag gtgccgattg attcacgcca cattccaaac gtgtctttcc
     1141 gtgcggtgat ggtttccaac tatcaggtga tgggcgattc ggtcgaagtg attgtgcctc
     1201 cgacgcgaaa tttcctgagt gtcgaggtca aagcggaccg tgaagaatat caatcacgcc
     1261 aggatggcca gttgaccatc acgacgcgtg acgccagtaa caaaccagtt tccgccgaag
     1321 tttcactggg gctggttgat gaatcggtgt tttatatcca gcaggagtat gcg
//
LOCUS       DAOQDO010002068         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_115565_length_1373_cov_2.709408, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002068 DAOQDO010000000
VERSION     DAOQDO010002068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115565_length_1373_cov_2.709408"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1373)
                     /locus_tag="PKZ53_25015"
     CDS             complement(<1..>1373)
                     /locus_tag="PKZ53_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ43767.1"
                     /translation="MLKYSLKHPILVIVVSVEVVASVVPVFQLIGKDFLPQDDQSRFE
                     VLVKLPEGNSLSAAQERIAQIEEEIKKIPHVIHVLSTIGGDEQRRVTRATLSVELKPI
                     SERKESQPKLMDLARKSLAKFSDIGIQIQNPAAISGGGQTNADVQYVIRGPEIETLTT
                     ASANLMSFMRNVKGVVDVDSNLEVGKPEMRAIINRPKASDLGVNVASIASALRTMVAG
                     EVVSSYREGDDRYDIRLRVEPTDRDSSIALLRTYVPSSKLGNVPLSNVVTMLDGTGPA
                     QINRYNRQRQVTVTANLQLGQSQSVVLTEIEKKIKELNLGPQYTAAPQGQSRELGRAA
                     QNFALAFILSFVFMYMILAAQFESFLHPVTILLSLPMAIPFALLSLLPFGQTLNIFSA
                     LGILMLFGVVKKNSILQIDHINTLRAEGMPRFEAIVEGCRDRLRPILMTTLALVAGLI
                     PMAVGQG"
BASE COUNT          323 a          364 c          363 g          323 t
ORIGIN      
        1 gccctggcca accgccattg ggattaaccc cgccaccagt gcgagcgtgg tcatcaaaat
       61 tgggcgtaac cgatctcgac agccttcgac aatggcttca aagcgcggca tcccttcagc
      121 tcgcaaggta ttgatgtggt caatttggag aatcgagttt ttcttcacca ccccaaacaa
      181 catcaaaatc cccagagccg agaaaatatt gagcgtctgg ccaaacggca gcagtgagag
      241 caaggcaaat gggatcgcca ttggcaagct gagcaggatc gtgaccgggt gcagaaagct
      301 ctcaaactgt gcggccaaaa tcatgtacat aaagacaaat gacaggataa acgccagcgc
      361 aaaattttgg gcggcccggc ccagttcacg gctttggccc tgtggggcgg cagtatattg
      421 aggcccaaga ttgagttctt tgattttctt ctcaatttcg gtgagcacca ctgattgcga
      481 ctgaccaagt tgaaggttgg cagtgactgt aacctgacgc tggcggttat agcggttgat
      541 ttgggcgggt ccggtgccgt caagcatggt gaccacgttg ctcaacggca cattccccag
      601 ctttgaggat ggaacatagg ttctcagcaa ggcgattgaa ctgtcccggt ctgttggttc
      661 aacgcgcaga cgaatgtcat accggtcatc gccttcgcga tagcttgaaa ccacttcacc
      721 tgccaccatc gtgcggagcg ccgaagcaat cgaggccaca ttgaccccga gatcagaggc
      781 tttgggacga ttgataattg ctcgcatctc tggcttccca acttcgaggt tggaatcaac
      841 gtccaccaca cccttgacgt tacgcataaa gctcatcagg ttggctgagg ctgtggtcaa
      901 ggtttcaatt tcaggtcccc ggatgacata ttgcacgtca gcattggttt gaccaccgcc
      961 ggagattgcc gccgggttct ggatttgaat cccgatgtct gaaaatttcg ccagactttt
     1021 gcgggccagg tccatcaatt tcggctgtga ttctttgcgc tcggaaatcg gtttgagttc
     1081 aaccgaaagc gttgcccgtg tcacgcgtcg ctgttcgtca ccgccgatgg tggaaagaac
     1141 atggataacg tgtggaatct ttttgatttc ctcttcaatc tgggcaatgc gttcctgggc
     1201 cgctgacagt gaatttcctt ccgggagttt gaccaggact tcaaagcggc tctggtcatc
     1261 ctgtggcaga aagtcctttc caataagctg gaacaccggc accaccgagg ccaccacctc
     1321 caccgacacc acaatcacca gaattggatg cttgagcgaa tacttgagca tgg
//
LOCUS       DAOQDO010002069         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_115719_length_1372_cov_2.911162, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002069 DAOQDO010000000
VERSION     DAOQDO010002069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115719_length_1372_cov_2.911162"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..737
                     /locus_tag="PKZ53_25020"
     CDS             432..737
                     /locus_tag="PKZ53_25020"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNJ43768.1"
                     /translation="MSSVGIRALKANLSAYLRTVQNGQEITVLDRNVPVARIVPIDPA
                     EEPESEEMELVAAGVLRMPKKPLPNDFWETAPAGLSPETLIQTISQERDEAEDRLLG"
     gene            709..1149
                     /locus_tag="PKZ53_25025"
     CDS             709..1149
                     /locus_tag="PKZ53_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNJ43769.1"
                     /translation="MKPKTAFWDSSALAPLCCQQPSSARARQLARRLTRQIVWWGTTT
                     EITSALARLDREGRLTPGLLRQHLEQLERLQNGWIEVLPVEQVRLTAADLLLRYPLRG
                     ADSLQLAAALAWCKQKPKRRPFVCFDERLAQVAEEVGFEVYRFE"
BASE COUNT          340 a          334 c          367 g          331 t
ORIGIN      
        1 caccgcgttg cgggatatcc actcgctgct tgaacgtggc attttggttc agaatccgag
       61 tggaggccgc agtactagtt atcggttagc ccaggaaagt gaaatcccga aagacgtctt
      121 ccgataatct catttccagt attcgcccca aaccctcacc taccgagtat tggtgaactc
      181 tcaaaagcca gtgtttatcg ctaccttgga acaccggaga atgtgtaatt cctgaaatat
      241 actttttcgg ggggatatgc aaaaacaccc aaattggctg gatgaagcgc tgtgaccata
      301 aagccggcca ataggcacaa aggctgttac ggaaactgct tgagctgctt caatgatttt
      361 gagtggacaa atttaccgca agggactaca gtgactacat tcttaaacaa cttggaaagg
      421 ggttttttgc catgtcaagt gttggaattc gtgcccttaa agccaatttg agcgcctact
      481 tgagaaccgt tcagaatggg caggaaatta cggtgctgga tcgcaatgta ccagttgcca
      541 gaattgtgcc gattgatccc gctgaggaac cggaaagtga ggaaatggag ttggtcgctg
      601 ccggtgttct gcgaatgccg aaaaaaccac tgccgaatga tttttgggag acggctccgg
      661 ccggattatc cccggaaaca ttgattcaaa ccatttcaca ggagcgcgat gaagccgaag
      721 accgcctttt gggatagcag tgcccttgcc ccactctgct gccagcagcc atccagcgcc
      781 agggcacgac agcttgcccg caggcttacc cgccaaatcg tatggtgggg aacaacaact
      841 gaaattacct ccgccctggc ccggttggac cgcgaaggac ggttgactcc ggggctgctc
      901 cggcagcacc tggaacagtt ggagcggttg caaaacggtt ggattgaagt actcccggtg
      961 gagcaagttc ggttaacggc cgccgacctg ctgctgaggt atccgttacg cggtgctgac
     1021 agtctgcaac tggctgcggc actcgcttgg tgcaaacaga aacccaaacg ccgtccgttt
     1081 gtctgttttg atgaacgcct tgcccaggtc gctgaagagg tggggtttga ggtataccgg
     1141 ttcgagtaat accgttactc ctttgagggt atccacccgg ctcaaaatag gttgattgac
     1201 cgcaaatttc atcattttca gtcaatatga tctttaattc aagataactg cactactcgg
     1261 tagatgagga ttgggagtga aaaggggaaa tgagattatc ggaaggcgtc tttcgggatt
     1321 tcactttcct gggctaaccg ataactggta ctgcgtcctc cactggaatt ct
//
LOCUS       DAOQDO010002070         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_115760_length_1372_cov_2.365983, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002070 DAOQDO010000000
VERSION     DAOQDO010002070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115760_length_1372_cov_2.365983"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKZ53_25030"
     CDS             complement(<1..625)
                     /locus_tag="PKZ53_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43770.1"
                     /translation="MPPKIRVALMYSDNTFASPINPEVTFGWGNYDDFGDEEAALVLE
                     MHRDGRFDVFNFTWQDITPELTFRQIHNLLTGERLEHVPIDETVDVIFVRGVGSSQRA
                     HDFRGTICERLQLLKQLSRTKVINPVETVISDLESKRYLVKLFSAGVPMPATYLARSL
                     AEIEEIGKQLSQGYVIKPVQGFGGFGIERFPGGSVAQVEQFLSTDGEV"
     gene            complement(849..>1372)
                     /locus_tag="PKZ53_25035"
     CDS             complement(849..>1372)
                     /locus_tag="PKZ53_25035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43771.1"
                     /translation="IERTQVQINGKSVDFTLKVNSEPGNLVIDPDSKLLRTSEEVRVA
                     VVVRRGIQHMEQEEYAEAQQQFEAAVKLNRRSSWAYYNLGLLYMKQQNFQRAIDAFDE
                     AYNGDLLPSWLEVWSLLYKGMAYDAAGQRDRAVAEYDKVQKLGDNPDNVQDLAQKYMS
                     APYRPERSTPSNP"
BASE COUNT          347 a          374 c          316 g          335 t
ORIGIN      
        1 gtacttcccc gtcggttgag agaaattgct caacctgagc caccgatccg ccgggaaacc
       61 gttcaatccc aaatccacca aacccctgga ccggcttgat gacatagcct tgtgaaagct
      121 gcttcccgat ttcttcgatt tccgccagtg atctcgccag ataggtcgcc ggcatcggaa
      181 cacccgccga aaacaatttg accagatatc gtttgctttc cagatccgaa ataactgttt
      241 cgaccgggtt gatgactttg gttcgactca actgcttcaa aagctgaagt cgttcacaaa
      301 tcgtgcctcg aaaatcgtgc gcccgttgtg agcttccgac accgcgcaca aaaatcacat
      361 ccaccgtctc gtcaatcggc acatgttcga gccgctctcc ggttaaaaga ttatgaatct
      421 ggcgaaaagt tagctccggc gtgatgtcct gccaggtgaa gttaaacacg tcaaaacgcc
      481 catcccggtg catttccagg accagcgcgg cttcttcatc gccgaagtcg tcataattcc
      541 cccacccaaa ggtcacttca gggttaatcg gagaagcaaa cgtgttatca gagtacatta
      601 aggcaacgcg aattttggga ggcatataaa agtcagtagt cagtagtcag tagtcaatcc
      661 gaaagcaagt ggctcagttt tgaattgaac tgatgttctg tcaaccagaa aaaaccaggg
      721 tgaatcgcgg gattcaacgc ttgaaccagg cctgatgact gtcaacgcct aaaaataaaa
      781 gcgagtagca agcttgggaa attgattgct cgctactcgc tactcactac tcactctttc
      841 actcaggctt acggattgga tggagtactc cgctcgggcc gatagggggc gctcatatat
      901 ttctgagcca gatcctgcac attgtccgga ttgtcgccaa gtttttgaac tttgtcgtat
      961 tccgcgactg cccggtcacg ctgcccggcg gcatcatagg ccattccttt gtagagcaac
     1021 gaccagactt cgagccacga cggcagcaaa tcaccattgt aggcttcatc aaaggcatca
     1081 atcgcccgtt ggaagttttg ctgtttcata tagagcaaac ccaaattgta gtaggcccaa
     1141 ctgctacggc gattgagttt aacggcagct tcaaattgct gttgagcttc ggcatattct
     1201 tcctgctcca tgtgttgaat accgcgccgg accaccaccg ccacccgaac ttcttccgag
     1261 gtgcgcagca atttgctatc cgggtcaatg accagattac ctggctcgga attaactttg
     1321 agcgtgaaat caaccgactt accattgatt tggacctgag tgcgctcaat cc
//
LOCUS       DAOQDO010002071         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_116057_length_1370_cov_3.147529, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002071 DAOQDO010000000
VERSION     DAOQDO010002071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116057_length_1370_cov_3.147529"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKZ53_25040"
     CDS             complement(<1..226)
                     /locus_tag="PKZ53_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43772.1"
                     /translation="MGVVLILSVSWPLFVQAEQLPITTFTTTNGLAQDRVKKIVRDSR
                     GYLWFCTADGLSRFDGYSFTTYGTDQGLPHQ"
     gene            663..>1370
                     /locus_tag="PKZ53_25045"
     CDS             663..>1370
                     /locus_tag="PKZ53_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43773.1"
                     /translation="MAHFQIKAENYPTRCEICHQADQFDPVTTICQRCQLAQTVAQVT
                     ITPTQPHVWLSKKAKIWLGIAGIILADTLCTLCYPLRMGVLFNVFSFITARQPFSSLE
                     FYQGRMCGMGAMASVAGVAIFYLLPAFFICCFLVVVILEVNGGKIPAWVQSASQQSDL
                     KPSQYPQIGSGQVLKPIFRQLLFGFIFNILAFVVLIVPQYSLAQTVRYDYGSLTRLFL
                     KLGVNVDQPDDHRWTALM"
BASE COUNT          336 a          327 c          331 g          376 t
ORIGIN      
        1 actggtgtgg caggccctgg tccgtgccgt aggtcgtaaa cgaatacccg tcaaaccgac
       61 tgagtccgtc cgctgtgcaa aaccagaggt acccgcgaga atcacggaca attttcttga
      121 cccggtcctg tgccagccca ttggtcgtgg taaaggttgt aattggaagt tgttctgcct
      181 gaacaaataa tggccagctc acgctgagaa taaggacgac gcccaatcgg agaaaccagg
      241 gcgaaactac cttgaagcgc tgacttgaga aagaagcaag acaaaaggcc atagtccttc
      301 tttgaaccag caagggcgtc tttcatgggg tgttttggat agttgtctgt gaagacaagt
      361 tcccaatacg tcgctgaaaa caaagtttgt tcacttgaaa acaagcacct tgagttcata
      421 tcaaggaatg atgtcaaaag aaaagagttt tctttcagat ttgagaggat gaggaataaa
      481 atggtgtttc tgcttcccac ccattcattt gcggatcttg cggaacaatt tgagttgctt
      541 tggactctaa ccccgaggat ccgttggaag ttgataccca ctgattccag aattttcctc
      601 ccggtttgat gacgtcattc caggattctg gtttcaattt cttcatgtca ggggatttga
      661 cgatggcaca ttttcagatc aaagccgaaa actatccgac gcgatgtgaa atctgtcatc
      721 aggccgatca gtttgacccg gtgacaacca tctgtcagcg gtgccaactc gcccagaccg
      781 ttgctcaggt gacaataact ccaacccaac cgcacgtctg gctctcgaaa aaagcaaaaa
      841 tctggcttgg gatcgctgga atcattctcg cggatacttt gtgtaccctc tgttacccac
      901 ttcggatggg ggtactcttc aatgtgtttt ctttcattac agcccgtcag ccattttcga
      961 gtcttgagtt ttatcagggc cggatgtgcg gtatgggggc aatggcctca gttgcaggcg
     1021 tagctatctt ttatctgctt ccagcctttt tcatttgctg ctttctggtg gtggtgatac
     1081 tcgaagtcaa tggcgggaaa atcccagcct gggttcaatc agcatcgcaa caatccgatt
     1141 tgaaaccctc ccaataccca caaattggct cagggcaggt acttaagcca attttcagac
     1201 aattgctatt cggatttatc ttcaacattc tggcctttgt ggttttgatt gtgcctcagt
     1261 attcacttgc ccaaacggtg cggtatgact acgggtctct gaccagattg tttttgaagc
     1321 tcggggtcaa tgttgatcag cccgatgacc accgctggac agccttgatg
//
LOCUS       DAOQDO010002072         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_116214_length_1369_cov_3.455860, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002072 DAOQDO010000000
VERSION     DAOQDO010002072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116214_length_1369_cov_3.455860"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKZ53_25050"
     CDS             <1..911
                     /locus_tag="PKZ53_25050"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ43774.1"
                     /translation="EVLRRSYIGRSVPVAAGGFSFELTVADTTGCLGTRQFTLTVDPA
                     APKISSFSPTSGRAGTSVVIVGARLTGATQVRFADKPATTVTVNSSGQITAIVPPGAT
                     TGPILVTGPGGSTVSTVAFTILNSAPTATDGTLSAKQNTPTTGKLTASDPEGTPLRFE
                     TVRSESTFRGIVTITNVTTGEYTYTPGRDFVGTESFFFRVFDGVAYSNLGRVTVTVAP
                     DTSPRISSVEVSGKDLVVNGENFGQGATVLVNGVAKAATNDAQNPTRKLVAKKAGKKI
                     KVGQTVTVQVRNPDGTTSAVFNFTRQ"
BASE COUNT          363 a          332 c          334 g          340 t
ORIGIN      
        1 ctgaagttct ccggcgatct tacatcggac gaagcgtacc agttgcagca ggcggctttt
       61 cgtttgagtt aaccgtggct gatacaaccg ggtgtcttgg tacccggcaa ttcacgctca
      121 cggttgatcc agccgcgcca aaaatctcct ctttttctcc aacaagtggg cgtgctggaa
      181 caagtgtcgt gattgtcgga gcgcgcctga ctggcgcgac acaggtacgg tttgccgaca
      241 aaccagccac aaccgtcacg gttaactcat ctggacaaat tacagcgatt gttcctccag
      301 gagcaacaac gggacctatc cttgtgacgg ggccaggtgg ttcgaccgtc agtacggtgg
      361 catttacgat tcttaattca gcaccgacag caaccgatgg aacactttcc gccaaacaaa
      421 acacgcccac aaccggaaaa ctgacagctt ccgatccaga aggaactccg ctccgttttg
      481 aaaccgtacg cagtgaaagt actttccggg ggattgtgac aatcaccaat gtgacaaccg
      541 gagagtacac gtacacgccc ggacgggatt ttgttggcac ggaatcattt ttcttccggg
      601 tatttgatgg tgtggcctac tcaaatctgg gacgcgtgac cgtgacggtg gcacccgata
      661 cttcaccgcg aatttcaagt gttgaggttt caggcaaaga tttggtcgtc aacggcgaga
      721 attttggtca aggggccacc gttttggtga acggtgttgc caaagcagcc accaacgatg
      781 cgcagaatcc aacccgaaaa cttgttgcca aaaaagctgg aaagaagatc aaggttggtc
      841 agactgttac tgtgcaggtt cgcaatccag acggaactac ctcggcggtg tttaacttta
      901 cccgtcaatg acaagtcacc tgctgtaaat ttttccgtga tttgattcac catattgaaa
      961 ttgatacttc ccgaaacccg cctggtgggt tcagaagatg tatcaagttc aaaccagggg
     1021 ttaaatcacg gaaaagttga aatcgctctt cagaacacag tctagagttc aagctttcgt
     1081 ttgttttggt cgaacggatc actcaactca aggtttatca agaacttaat caacacgagg
     1141 gggggcgatt tcgcaaactg aagcctggac tctggactga aatcattttt tcaagacagg
     1201 tagcagaaag ttcagtattg tggtcgaatt ttgggtcaaa ccctgaattg cttgctggaa
     1261 gagtcgtttt gaattgcatt ttatttccag acagagaaaa tcccaccatc aatccaccaa
     1321 acagtgtaat cgttttatcc taactcaata actcctgagg tcgtatgga
//
LOCUS       DAOQDO010002073         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_116291_length_1369_cov_2.213851, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002073 DAOQDO010000000
VERSION     DAOQDO010002073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116291_length_1369_cov_2.213851"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKZ53_25055"
     CDS             complement(<1..724)
                     /locus_tag="PKZ53_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ43775.1"
                     /translation="MKFTEEHQMFRESLRRFIEKEINPFVEEWEQAEIFPAKAIIAKM
                     GELGFLGVNYPEEYGGLGLDYWYNVIRAEELGRIPCGGVPMGISVHTDMCTPALADFG
                     SHELRKKFLEPSIKGELLGAIGVTEPDAGSDVSSIRTRAESDGDHYVITGTKLYITNG
                     TQADWICTLVRTSPGTGFDGMSLVIVPTDAPGFSVSRKLKKMGNYSSDTAELSFDRVR
                     VPKWYRIGPEGKGFMLQMQQFQK"
BASE COUNT          341 a          366 c          331 g          331 t
ORIGIN      
        1 ccttctgaaa ctgctgcatt tgcaacataa agcctttgcc ttcggggccg atgcggtacc
       61 acttgggaac acgaacccga tcaaatgaca attcggctgt gtcgcttgaa taattcccca
      121 ttttcttgag cttgcggctc acgctaaatc ctggggcatc cgtcggcaca atcaccagcg
      181 acatcccatc aaacccggtg ccaggcgaag tccgcaccag cgtacaaatc cagtcggcct
      241 gggtgccgtt ggtgatatag agtttggtgc cggtaatgac atagtggtcg ccatctgatt
      301 cggcgcgggt gcgaatgctt gaaacatcag agccagcgtc gggttcggtc acgccaatcg
      361 cacccagtaa ttcaccttta atggacggct ccagaaattt tttccgaagt tcatgcgagc
      421 caaaatcagc cagcgccggc gtgcacatat cggtgtggac cgaaattccc attgggacgc
      481 cgccacaggg aatgcggccc agttcttcag cccgaatcac gttgtaccag taatccagac
      541 ccaacccgcc gtactcttcc gggtaattga cacccaaaaa ccccagttcg cccattttgg
      601 cgataatcgc tttggcggga aagatttccg cctgctccca ctcttccaca aaggggttga
      661 tttctttttc gataaagcgc cggagcgatt cccgaaacat ctgatgttct tcggtaaatt
      721 tcatgaacag caaactcctt attgaaatga aaaattgcgc cacccgcctg aatataaaga
      781 aactcggttg aggtttttcg ctgaggacgt tctgggaacg gtgacaaagg ctggcagatg
      841 aaaccacagt cgtgacttcc ccaggtttca ggtatgagga acaagccagg gtctggggat
      901 gtcaatgcct caaatgatag cagagggatt cctggaaacg gtagcggcat cttggtgacc
      961 tgacccgaaa gcaaccggca aatcaaacca aaccagtcaa ctcaatggat tggcacattt
     1021 ttttggtgtc caggtgaaaa ctgttgctgg gagtcaggcc gtacccgcgc tcattccaca
     1081 aaagaatcca ctcaaaatca acttttgacc tgttttcact gtcttggggg caggtgggaa
     1141 aactcatttt ttagaaattt tggagaaccg aaatgacttt aggcggactt gcagtttttt
     1201 ctttcagaat caaacacatg gtgactcgga tcgcgacttc tcgccggttc ttgatttttc
     1261 cggtggttgt aatcagcctg tttctgaccc attcacgtgc cgttacatca caggcccaag
     1321 ctccagttga tcaggccagg tcagacccga aaccccagtc atcaccagc
//
LOCUS       DAOQDO010002074         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_116514_length_1368_cov_1.923077, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002074 DAOQDO010000000
VERSION     DAOQDO010002074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116514_length_1368_cov_1.923077"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKZ53_25060"
     CDS             complement(<1..182)
                     /locus_tag="PKZ53_25060"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNJ43776.1"
                     /translation="MTKETHHLRMYLMRHGEIDATHRQFAYGSRDINLSTTGEEQSHW
                     MCQQLSGVKLDAVYSS"
     gene            complement(179..991)
                     /gene="cobS"
                     /locus_tag="PKZ53_25065"
     CDS             complement(179..991)
                     /gene="cobS"
                     /locus_tag="PKZ53_25065"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNJ43777.1"
                     /translation="MTNNRLHSSFFILHSSFGEMLKLFFIAIQFLTRLPVPISHATTE
                     EEIGRATAFFPMVGLIVGGSAAGIAMLAHPFFPHSTCVLAALVWMTLLTNGFHEDGLA
                     DSFDGFGGGWGKDQVLTIMRDSRIGSFGSLALIFLILAKYNLLSNLSFDQVWRWLIVA
                     HVASRWTVLPLARWLPYARPEGQGKLVAKQINGQTLVFGTLTLVGTVALILPVKIAVT
                     ALMIPLAVVILSGLYYRERIGGLTGDCLGATNQLTEVSLYVTAVALRNMFSL"
     gene            complement(1129..>1368)
                     /locus_tag="PKZ53_25070"
     CDS             complement(1129..>1368)
                     /locus_tag="PKZ53_25070"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNJ43778.1"
                     /translation="ALAVAIQPAVCEYLFLAHRSVEPGHAALLDFIGLPPILDLQLRL
                     GEGTGAALAMPILESAARILSEMATFSEAGVSNQE"
BASE COUNT          375 a          347 c          324 g          322 t
ORIGIN      
        1 tcacttgaat agacggcatc cagtttgacc ccggaaagtt gctggcacat ccagtgggat
       61 tgttcttcac cggtggttga taagttgata tcgcgtgagc cgtaggcaaa ttggcgatgt
      121 gttgcatcaa tttcaccgtg gcgcatcagg tacattcgaa gatggtgtgt ttctttcgtc
      181 atagagaaaa catatttcgc agtgcgactg ccgtcacata gagcgaaacc tcagtcagtt
      241 gatttgtggc acccaggcaa tctccggtca ggccaccaat ccgttcacga tagtacaaac
      301 cgctcaagat caccactgcc agaggaatca tcagagcggt caccgcgatt ttcactggta
      361 aaatcaaagc caccgttccc accagcgtca acgtgccaaa gaccagtgtc tgcccattga
      421 tctgtttggc aacgagtttc ccctgtcctt caggtcgggc gtagggaagc catcgtgcca
      481 gtggaagaac ggtccaccgg ctcgccacat gagccacaat caaccatcgc cagacctggt
      541 caaatgacag attcgaaagc agattgtact tcgccagaat aagaaaaatg agtgccagcg
      601 aaccaaacga accgatccgg ctgtcacgca taatggtcaa aacctgatct tttccccagc
      661 cgccgccaaa tccgtcaaag gaatccgcca gtccgtcttc gtgaaagcca ttggtcaata
      721 aagtcatcca caccagcgct gccagaacgc aggtcgagtg gggaaaaaat ggatgtgcca
      781 gcatggcaat tcccgccgca ctcccgccca caatcaagcc aaccattgga aaaaaagcag
      841 ttgcccggcc aatttcttct tctgtcgtgg cgtgagaaat cggcaccggc aaccgtgtca
      901 aaaattgaat tgcaatgaaa aagagtttga gcatttcgcc aaatgaagaa tgaagaatga
      961 agaatgaaga atgaagccga ttgtttgtca aatcagttta ttgctaaata tactttgttc
     1021 ttattaaatg gtttacaagg attttcccca ggaagcttta ctgcttgctc atgcgagtct
     1081 gtcattccaa aatcatataa ctggctttat ttcttcattc ttcattcttc attcttgatt
     1141 gctgactccg gcttcggaaa atgttgccat ttctgaaagg attcgggcag ccgattcaag
     1201 gattggcatt gcgagcgctg cgccagttcc ttcaccaagt cgaagttgaa gatcaaggat
     1261 cggtggcaac ccaatgaaat ccagcaaggc ggcatgtcct ggttcaactg agcgatgcgc
     1321 cagaaacagg tattcacaaa cggcaggctg gatggcaacc gcgagggc
//
LOCUS       DAOQDO010002075         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_116626_length_1367_cov_2.408537, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002075 DAOQDO010000000
VERSION     DAOQDO010002075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116626_length_1367_cov_2.408537"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1367)
                     /locus_tag="PKZ53_25075"
                     /pseudo
     CDS             complement(1..1367)
                     /locus_tag="PKZ53_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525473.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          287 a          372 c          375 g          333 t
ORIGIN      
        1 ccgctgtgag ctgggtttcg cctttcgggg ttactttgcc caccagaatt gaccccggtt
       61 tgacccgggc gccaatgcgg atgatgcctg actcatccag gtcacgcagc attgactctg
      121 agacgtttgg aatatcgcgg gtgatttctt caggtcccag cttggtgtcg cgggcttcga
      181 tttcgagttc ttcaatgtga atcgaggtgt aggcatcttc ccgcaccagc ttttcagaaa
      241 tcagaatcgc gtcttcgaag ttgtatccgc gccacggcat aaacgacaca gcacgttgcg
      301 gcccagggca agttctccga aatcagtaca cggaccgtct gccagcacat cacctttttt
      361 gacccgctgt ccgacttgca caatcggctt ctggttgatg caggtgctct ggtttgagcg
      421 acggaacttg accagttgat aaatatcggc ggtcacttca cgcgagagtg atccggactg
      481 ggtattgtca acccggatga taatgcgttc tgagtcaacg ctatccacca cgccatcacg
      541 acgggccagc acaacggcac cggaatcctg ggcggtgact ttctccatcc cggttccaat
      601 caacggggct tcggctcgca ggagcggcac tgcctgacgt tgcatgtttg atcccatcag
      661 cgcgcggttg gcgtcgtcgt gttcgagaaa cggaatcaac gccgccgcca ccgataccaa
      721 ctgtttgggt gaaacgtcca caaagtggat ttcatgacgg gctgattctt tgaaatcccc
      781 ctggttgcgt gatgaaacac gttcgttgag gatcacattg ttttcatcaa ccgtgatgtt
      841 tgcctgtcca acggtgtatt tatcttcttc ccaggctgaa agataaaacg gatgtggctc
      901 gtaatcggcg gtcttcttcc ctggtgcgag cgctttattg atgcgattga cttcatcggc
      961 gggcaggtgc atctcaggct tcagttcagt gtcaccacca ttggtaatgc gcacatattc
     1021 gatcacccga ccattttcga ctttgcggta gggggattcg ataaagccaa attcattgat
     1081 tcgggcaaaa cacgacagcg atgaaatcaa cccgatgttc ggaccttcag gggtttcaat
     1141 cgggcagata cgaccatagt gcgtgggatg cacgtcgcgg acttcaaacc cggctcgttc
     1201 acgcgaaaga ccgcccggcc cgagggccga cagacgccgc ttgtgggtga tttccgacag
     1261 cgggttggtt tggtccataa actgcgacaa ctgagatgag ccaaaaaatt cgcgcaccgc
     1321 agccgtgacc ggcttggcgt tgatcaggtc gtgcggcatc gcggtct
//
LOCUS       DAOQDO010002076         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_116768_length_1366_cov_3.196796, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002076 DAOQDO010000000
VERSION     DAOQDO010002076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116768_length_1366_cov_3.196796"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1366)
                     /locus_tag="PKZ53_25080"
     CDS             complement(<1..>1366)
                     /locus_tag="PKZ53_25080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43779.1"
                     /translation="YFELPFGKLVWYPQVWTLIGLGALMFLMLAVGLIGVRSQQLTLS
                     GIFLGILAMMTVLVPPALVVWGLWSLIHALSAHHQAFAYFEDAYQQHWYLLGFTSLTV
                     AIISALWMTASQKVSWPNLAYGALFIWTVLAIIAIRFFPGGAFLFMLPALASALSLGI
                     LFVRQWDDWTAWRPGVVLSLGMLPALYFMSPLILCLVVALTLKLAWVAMIVVGLLLGL
                     LLPQIAVLHQTVPKRLPLLALTVGVICLITGGLKTRVDYDHRQPNMIFYALNADTNQA
                     FWVSDLSPVDDWERQFLKDTPTRRSLPDFFPISSSTFQVSPASVAPIEPALAEVLSDE
                     RTADSRKVRLRVRSPRLAERLSLYFEDTELRSLAVNGKAFQKLPTYQAESGPTYTFDY
                     VAPPADGLVIDLETVGTNPIKLVMVDQSLRLPPLPGFIFIHRTEEMMPSIRSLTDSTF
                     VVKTV"
BASE COUNT          378 a          364 c          353 g          271 t
ORIGIN      
        1 tcaccgtttt taccacaaag gttgaatctg tcaggctccg aatgctgggc atcatctctt
       61 cggtgcggtg gataaaaata aatccaggaa gcggtggcag gcgcaacgat tgatctacca
      121 tcaccagctt gatggggttg gttccgacag tttcaagatc aatcacgagc ccatcagcgg
      181 gcggggccac atagtcaaaa gtgtaggtcg gaccagattc cgcctgatat gtggggagtt
      241 tttggaaggc tttgccattc accgccaaag atcgaagttc tgtgtcttca aaatagagcg
      301 acaaccgttc tgccagtcgc ggagaccgga cccggagccg tacttttcgc gaatcagccg
      361 tgcgttcgtc agataagact tcggcgagag ctggctcaat cggcgccacg gaggccggac
      421 tgacctgaaa ggtacttgaa cttatcggaa aaaaatctgg gagcgaccga cgtgttgggg
      481 tgtctttgag gaactggcgt tcccaatcgt ccaccgggct caggtctgaa acccagaacg
      541 cctggttggt gtcggcattc aaggcataaa aaatcatgtt tggctgacgg tgatcatagt
      601 cgactctggt tttcaaacca cctgtaatca ggcagataac accaactgtc agtgccagga
      661 gtggaagtcg tttcggcact gtttggtgca aaactgcaat ctgtggaagt aacagcccga
      721 gcaaaagccc caccacaatc atggcaaccc aggccagctt gagggtcaat gccaccacca
      781 gacacaaaat gagcggtgac ataaagtaca acgccggaag catccccagg ctcaacacaa
      841 ctccaggtcg ccaggcggtc caatcgtccc actgtcgcac aaagagaatt cccaggctca
      901 aggcacttgc cagcgccgga agcataaaca gaaacgctcc tcccggaaaa aatcgaattg
      961 cgataattgc cagcactgtc cagatgaaca gtgccccata ggccagattg ggccacgaga
     1021 ctttttggct ggcggtcatc cagagcgctg aaataattgc aaccgtaagg gatgtaaatc
     1081 caaggagata ccagtgctgc tgataagcat cttcaaaata cgcaaatgcc tgatggtgag
     1141 ccgaaagcgc atggattaaa ctccataaac cccacaccac cagggctggc ggcaccaaca
     1201 cggtcatcat tgccaggatc ccaaggaaga ttcccgacag ggtgagttgc tggctgcgaa
     1261 ctccgatcaa tcccacggcc agcatcagga acatcagtgc tcccaaccca atcagagtcc
     1321 aaacctgtgg gtaccagacc agttttccaa agggaagttc aaaata
//
LOCUS       DAOQDO010002077         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_117483_length_1362_cov_3.604438, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002077 DAOQDO010000000
VERSION     DAOQDO010002077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117483_length_1362_cov_3.604438"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1362
                     /gene="selB"
                     /locus_tag="PKZ53_25085"
     CDS             33..>1362
                     /gene="selB"
                     /locus_tag="PKZ53_25085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNJ43780.1"
                     /translation="MKNIIVGTAGHIDHGKTSLVKALTGTDTDRLKEEKQRGITIDIG
                     FANLTLNGFQLGFVDVPGHERFVKNMLAGAHGIDLVMLVIAADESVMPQTREHLEICQ
                     LLRIPTGLVVLTKIDMVDAEWLELVQSEVETFVDGTFLEGKPVIAVSTRTGDGIDELK
                     QALAQIALTVTEKSSARPPRLPIDRVFSVKGFGTVVTGTLIAGSFRVGDDVEIHPGGR
                     RTKIRSIEVHGQSSERATAGQRTAINLQGVDIETVTRGQVVTPAGRFAATPMIDVQLE
                     LLASVEKPLRHRTRVRFHHGTSEVMARVHLLNPPADLASKNRELGPGQRCFAQLHLEE
                     PVLALPGDRFILRHYSPQFTIGGGLVLDVQPQRHRASEREGVTLFLAKLSSSDSAVRV
                     AAFLEKSGPSGVTLAELAARSGESDAVLKAVLTRLHQQNQLVFIDTANGKA"
BASE COUNT          325 a          336 c          379 g          322 t
ORIGIN      
        1 actactgact cctgactact gactgatctc atatgaaaaa tatcattgtt ggcacggcag
       61 ggcatattga tcacggtaaa acatcgctgg tcaaagcact taccggaaca gacactgatc
      121 gtctcaaaga agaaaaacaa cgcggtatca cgattgatat tggatttgca aacctgacac
      181 tcaatgggtt tcagttgggg tttgttgacg tgccggggca tgagcggttt gtgaaaaaca
      241 tgctggctgg tgcacatggc attgatctgg tcatgttggt gattgcggct gatgagtcgg
      301 tgatgccgca gaccagagag catctcgaaa tttgccagtt gctcaggatc ccaaccgggc
      361 tggtggtact gaccaaaatt gacatggttg atgccgaatg gcttgaactg gttcagagcg
      421 aagtcgaaac atttgttgat ggaacattcc ttgagggcaa accagtcatt gcggtcagta
      481 ctcgcaccgg tgacggcatt gatgagttaa agcaggcgtt ggcacagatt gcgctcaccg
      541 ttaccgaaaa aagcagtgct cgcccgcccc gcctcccgat tgatcgggtg ttttcagtca
      601 aagggtttgg gaccgtggtc accggaaccc tcattgccgg aagttttcga gttggggatg
      661 atgtcgaaat tcatccaggt ggacgccgaa ccaaaattcg aagtatcgaa gtccatgggc
      721 aatcctctga gcgtgccact gccggacagc ggacagccat caacttgcaa ggcgtagata
      781 ttgagaccgt cacgcgtgga caggtggtca cgccggctgg gcggtttgcg gcaacgccca
      841 tgattgatgt ccagttagag ttgctggctt cagttgaaaa accattgcgt catcggaccc
      901 gcgtacggtt ccatcacgga acaagcgagg ttatggcgcg agtccatttg ttaaatccac
      961 cagccgatct ggcctcgaaa aatcgcgagc ttggtcccgg gcaacgttgt tttgcccagc
     1021 tccacctcga agaacccgtg ctggctttac ctggtgaccg gtttattctg cggcattatt
     1081 cgccccagtt cactatcgga ggcggactcg tgcttgacgt tcagcctcaa cggcatcgag
     1141 ccagcgaacg tgaaggggtt actttatttt tagcaaaact ttccagttca gattcagccg
     1201 ttcgagtggc cgcgttcctg gagaaaagtg gtccttcagg ggtgacactg gctgaactgg
     1261 ctgccagaag tggtgaatcc gacgcggtac tcaaagctgt cctgacgcgg ctgcaccagc
     1321 aaaatcaact cgtgtttatt gacaccgcca atggaaaagc cc
//
LOCUS       DAOQDO010002078         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_117660_length_1361_cov_2.822358, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002078 DAOQDO010000000
VERSION     DAOQDO010002078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117660_length_1361_cov_2.822358"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKZ53_25090"
     CDS             complement(<1..773)
                     /locus_tag="PKZ53_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43781.1"
                     /translation="MRFQLPYFVFSILLLIDFPLVVHNEDFSDSRRILNPKWNFPRPV
                     IQDSPPQDTTESSSIGLEALSPYAIAKLITDDPLALDDIWNQLGIHSLHLGYEGISTD
                     RLQSPDQFLTSPIVNVKTYTDELDGEPGNEVLLRVSNNTSEECRYVLFKPLKRRAGKP
                     RWKLLGYIDHDFGRYQMPEHSIAQSGGRKWLMITVQMGSGTGYARYDNRLFSVDHTGL
                     KEILQFPSEEIFACCDNYPARKFKTKLVRCEMSGATTTVT"
     gene            complement(1140..>1361)
                     /locus_tag="PKZ53_25095"
     CDS             complement(1140..>1361)
                     /locus_tag="PKZ53_25095"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNJ43782.1"
                     /translation="ERYTITYRLKDIELRLDSAQFVRLSRSAIVRINAIRSATPMPGG
                     TYLVSLTNGQQLHSSRSQSRILREDLLRL"
BASE COUNT          369 a          307 c          328 g          357 t
ORIGIN      
        1 gtaacggttg tggtcgctcc gctcatttca caccgtacca gctttgtttt gaacttcctc
       61 gctggataat tatcgcagca tgcgaaaatt tcttcagatg gaaattgcaa tatttctttt
      121 aagcctgtgt gatcaaccga aaacagccgg ttatcatagc gtgcataccc agtcccagac
      181 cccatttgaa ccgtaatcat cagccatttc ctgccaccac tctgagcaat tgagtgctca
      241 ggcatctggt atcttccaaa gtcatgatca atatatccga gcaatttcca tcgtggtttt
      301 ccagcccggc gttttaatgg tttgaacaga acatagcgac actcttcaga ggtattgttt
      361 gagactctga gcaaaacttc gttccccggc tcgccatcga gttcgtcagt atatgttttg
      421 acgttaacaa tgggggaggt taaaaactga tctggtgact gtaaccggtc ggtacttatt
      481 ccttcatatc caagatgtaa actgtgaata cccaactgat tccaaatatc gtctaaagcc
      541 aacgggtcat cggtgatgag ttttgcaatc gcatagggtg ataatgcctc caacccaatg
      601 gaactggatt cagttgtgtc ttgtggcggc gagtcctgta tgactggtcg aggaaagttc
      661 catttgggat tcaaaattct tcttgaatca gaaaaatcct cattgtgcac aaccagagga
      721 aaatcaatga gaagcaaaat tgaaaagaca aagtagggaa gttggaacct cattggcgaa
      781 actcctggtg tggcagatat agattttcag aataaaaaac cacggagcca cggaatacag
      841 agaaaaccaa accagatcct tcgctgattg gtgaggtttt aatggggtaa ctatcgggga
      901 aatattgatc tgaaaagcga tcaatcaatc aatttggcga cctggccgcc agattagctt
      961 gaactggagg ttgcctcact gtgtgcatca ccagtcagca tcaattgatc accacaactc
     1021 gcaatgagtc tggggctgaa aaaacagggc tgagggcttg gggctgaaga aaatgggttc
     1081 aatcccccga gcccgttttt tcagcccgac ttcttcagcc aaaattggtg ttactttcat
     1141 taaagccgca gcaggtcttc gcgtaagatg cgcgattgcg agcggctgga atgcagttgc
     1201 tgaccattgg tcaaactgac cagataggtg ccgccgggca ttggggtggc gctccggatg
     1261 gcattgatac ggacaatcgc gctccggctg agtcgcacaa actgcgccga atcaagccga
     1321 agttcaatat ctttgagtcg gtaggtaatt gtgtagcgtt c
//
LOCUS       DAOQDO010002079         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_117671_length_1361_cov_2.710567, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002079 DAOQDO010000000
VERSION     DAOQDO010002079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117671_length_1361_cov_2.710567"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKZ53_25100"
     CDS             <1..190
                     /locus_tag="PKZ53_25100"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNJ43783.1"
                     /translation="TADWSLFSLSPGTSAVDLKRAYRDLIDVWHPDRFAHNPRLQAKA
                     EAMTKRINAAYQRLQVAA"
     gene            complement(435..>1361)
                     /locus_tag="PKZ53_25105"
     CDS             complement(435..>1361)
                     /locus_tag="PKZ53_25105"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HNJ43784.1"
                     /translation="VRSRILTPLEMNQTGFTIADLEREPDHAIPVMETRDGGKLYRLP
                     FYRELQGIAPAGAIHSNVEELSHWVIALLNDGMYRGRQILPASVLKATLEPAIVCQNK
                     DLENGFSEVLNPTYGMGRWIASYRGHRLTYHGGSIDGYISQISMMPQEKIGVILLFSG
                     EHVWGLHEVVSYQIYDRLLGLNVTPWNERRLATRLKEAQAEKDARSKVDKDKVPNTKP
                     SHALADYVGEYEHPVYGILKIGLKDGQLHLESHKLQFPLIHFHYDRFDTPDDEYYGRW
                     SVNFSTNLKGVIEKAGMTMNDSEWVFTRRSNR"
BASE COUNT          368 a          349 c          286 g          358 t
ORIGIN      
        1 aacagcagac tggagtcttt tcagcttgtc ccctgggaca agtgccgttg atctcaagcg
       61 agcctaccgg gatctgatag atgtttggca tccagatcgc ttcgcacata acccccgatt
      121 gcaggcaaaa gctgaagcca tgaccaaacg catcaatgcc gcgtaccaac gccttcaagt
      181 agcagcttaa atcaaactaa gtctgataac tgaaaaaaaa atctcaattt acattgtatc
      241 agtcaatttc ttagccttgc ctgtcattat cggactcttg atcattatgc ccaattcttg
      301 ccagtaacca atcccttcag tggtttaaat acttcatcaa gcgctaaccc cttgttgaaa
      361 cacctcttac aacccttctg atttacctcc gacacaacat ccagtagaaa ccgaaaacta
      421 aagccagaat ggaactaacg gtttgaacga cgggtgaaga cccattcaga atcattcatc
      481 gtcatacctg ctttttcaat cacgcctttc aaattggtcg aaaaattgac tgaccaccgg
      541 ccatagtatt catcatcagg cgtatcgaac cgatcataat ggaaatggat caacggaaac
      601 tgcagtttgt gagattcaag gtgcagttgg ccgtctttaa gaccaatttt taaaattccg
      661 tataccgggt gttcatattc accgacataa tcagccagag cgtgagaagg tttggtattg
      721 ggcactttat ccttgtcaac tttggatctg gcatctttct ccgcctgggc ttctttcaac
      781 cgggtagcca gccgccgttc gttccagggc gtcacattca atcccagcaa ccggtcataa
      841 atttgatagc tgacgacttc gtgaagtccc caaacgtgtt caccgctgaa aagcaaaatc
      901 acgccgattt tttcctgagg catcattgag atttgcgaaa tatacccatc aattgacccg
      961 ccgtgataag tcagccgatg cccccggtag gaagcaatcc atctccccat tccgtaagtt
     1021 gggttgagaa cctctgaaaa accgttttcg agatctttat tttgacagac aattgctggt
     1081 tcgagggttg ctttgagcac actggctggc aggatttggc gacccctgta catcccatca
     1141 ttcagcaggg caatgaccca gtgggagagt tcctcaacat ttgaatgaat ggctccagcg
     1201 ggagcaatgc cttgcaattc acggtaaaaa ggaagtcgat agagtttccc gccatccctg
     1261 gtttccatca ccggaatggc atgatccggt tctcgttcaa ggtcagcaat cgtaaacccg
     1321 gtctggttca tttctaatgg agtgagaatt cgactccgga c
//
LOCUS       DAOQDO010002080         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_117756_length_1361_cov_1.888208, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002080 DAOQDO010000000
VERSION     DAOQDO010002080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117756_length_1361_cov_1.888208"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1361)
                     /locus_tag="PKZ53_25110"
     CDS             complement(<1..>1361)
                     /locus_tag="PKZ53_25110"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ43785.1"
                     /translation="GPLTTLIDQSFEFDTYPLLRMVGDESTESSLHGRMVLEFNNQLD
                     FKTFRPEMVTISPPIPSAQITVWQNQILIDGKKPYVPVYTVTVSGDLTDQSGQRLEQP
                     VSRQFKIRLAQPDIHFDYGVITTFDPGGLHQLNVYSQNIQTLNVRLYRVTADDWNAYA
                     SPGVYGWAKTNEVTKAILREPVFSQSIPVKVDLDEVIQGQIDLTPALPNGTGQILVEV
                     SGDVAPGDFDIHTVRAFHWVQSSKLAVDVVAGGSELLVWANSLSDGKPVSGATVTAYP
                     GGETVPVDADGLGRFPFQADIHEYNRFVVRKGDDSAILCQTYERWYCQPPGISAIHWH
                     LLSDRTLYQPGEDIHLKGWARVFKTNQTEPIEPVGNAFQSVSYTITDPRGVKLGEGSV
                     PVNAFGGFYVKGQIPKTAATGAASIHLSADLPDTSSLFTGQDTQHPFQIEEYQRPDFE
                     VTV"
BASE COUNT          345 a          338 c          339 g          339 t
ORIGIN      
        1 aacggtgact tcgaaatccg gtcgctgata ctcctctatt tggaacggat gctgtgtgtc
       61 ttgacccgta aacaagctgg aggtatcagg gagatctgcc gaaaggtgga ttgacgcggc
      121 gccggtggcg gcagtttttg gaatttgacc tttgacataa aaccccccaa aagcattgac
      181 gggcactgat ccttcgccca gcttgacgcc tctcgggtca gtaatggtgt aggacactga
      241 ttgaaaggcg ttgcccaccg gctcgatagg ttcagtctga tttgtcttga agacacgagc
      301 ccaacctttg agatgaatgt cttctccggg ttgatacagg gtacgatcac tcaaaagatg
      361 ccagtgaatc gctgatattc caggtggttg gcaataccag cgttcatatg tttgacacaa
      421 gatggctgaa tcatcaccct tgcgaaccac aaaacggtta tattcgtgaa tgtctgcctg
      481 gaacgggaat cgccccaggc catcagcatc aactggaacg gtttccccgc caggataggc
      541 cgtgacagtt gccccggaaa ctggttttcc atcactcaga gaattggccc acaccagcaa
      601 ctcagagcca ccggcaacga catccaccgc cagtttggac gattgtaccc agtggaaagc
      661 cctgacggtg tggatgtcaa aatcaccagg ggcgacatca ccgctcacct caaccagaat
      721 ctgacccgtt ccgttgggca aggccggagt caggtcaatt tgcccttgta taacttcgtc
      781 gaggtcaacc ttgaccggta ttgattggga aaatactggt tccctgagaa ttgctttggt
      841 gacttcattt gtttttgccc aaccatatac ccctggagaa gcataggcat tccagtcatc
      901 agccgttaca cgatagagcc tgacattcaa cgtctgaatg ttttgcgaat atacattcaa
      961 ttggtgcaag ccgcctgggt caaaggttgt gatcacccca tagtcaaagt gaatatccgg
     1021 ctgagccagc cgtatcttga attgacgact cactggttgc tcaagtcgtt gtccagattg
     1081 atcggtgaga tcaccagaaa cagtcacggt ataaacggga acatagggtt tttttccatc
     1141 aatcaagatc tggttctgcc aaacagtaat ttgagcggat ggaattgggg gtgaaatggt
     1201 taccatctct gggcgaaatg ttttgaaatc cagttgatta ttaaactcca gcaccatcct
     1261 cccgtgaagc gaactctcag tactctcgtc cccaaccatc cgcaagagag ggtaggtatc
     1321 gaattcaaat gactggtcaa tcagcgttgt gagtggtcct t
//
LOCUS       DAOQDO010002081         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_117848_length_1360_cov_2.731034, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002081 DAOQDO010000000
VERSION     DAOQDO010002081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117848_length_1360_cov_2.731034"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1231)
                     /locus_tag="PKZ53_25115"
     CDS             complement(233..1231)
                     /locus_tag="PKZ53_25115"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNJ43786.1"
                     /translation="MPTLKSPAIFNNPECLVEGWYWALRSAELKLKAVKGLMFLGREL
                     VVYRGESGMVSALDAFCPHMGAHLKEGMVEGDSLRCLFHYWKFDAQGNCVEIPCQEKC
                     DFVPALKTWPVVEKYGLIWIWAGKTASQPVPCVPELKDLPCAARLGNRFVKNCHPNVV
                     MINAIDAQHFNSVHNLPVDLDLQPTVINKNCIEFRNTTQIPHNTWMTRFLARFYQKAL
                     TYELCYWFGSTGTVTLGPDFLHFHIMFALRPTPDGKTEGQTLLITRERPGMFGGLINS
                     GLLVLTQIVSNYFAKGDTLIFESIRFHFRTPIKADSAIIEFIQHLEQQSATTWGNW"
BASE COUNT          342 a          325 c          358 g          335 t
ORIGIN      
        1 gttcagggtt cagggttcag ggttcagggt tcagggttca gggttcaggg ttcaagggtt
       61 tcgaaagaat ataaggataa aggatgaaaa aagtcactct gtcaccttgt cataccattt
      121 tgggatcagg ggtcagggaa atacgatgtt gtctgttccc tcatggtttt tgtttgattt
      181 attcccaaaa tttgaaaccc tgaaccctga accctgaacc ctgtctagtc tgtcaccagt
      241 tcccccaggt tgtggctgat tgttgttcca gatgctgaat aaactcgatg atggcactgt
      301 cggctttgat tggggtgcga aagtgaaagc ggatgctttc aaaaatcagg gtatcacctt
      361 tggcaaagta atttgagaca atttgggtga gtaccagcag tccagagttg atcagccccc
      421 cgaacatacc cggtcgctcg cgggtaatca acagggtttg gccttcggtt ttgccatcag
      481 gcgtcggacg aagcgcaaac ataatgtgaa aatggagaaa atctggcccc aaggtgaccg
      541 ttccggtact gccgaaccag taacacaatt cataggtgag tgccttctgg taaaaacgag
      601 ccagaaaacg ggtcatccag gtgttgtgag ggatttgggt cgtattgcga aactcgatac
      661 agtttttatt gatgacggtc ggttgcaaat ccaggtctac cggcaggttg tggaccgagt
      721 tgaaatgttg ggcatcaatg gcattgatca tcacgacatt gggatgacag tttttgacaa
      781 accgattccc cagccgggca gcacagggca ggtctttgag ttccgggaca catggcaccg
      841 gctggctggc tgttttcccg gcccaaatcc agattaaccc gtatttttcc accacgggcc
      901 aggttttcag cgctggcaca aaatcgcatt tttcctgaca gggaatctca acacagttcc
      961 cctgggcatc aaatttccag tagtgaaaca gacaccgcag gctatcgcct tcaaccattc
     1021 cttctttgag atgggccccc atatgcgggc agaaggcgtc cagagcgctg accattccgc
     1081 tttcacctcg atacaccacc agttcccgcc ccagaaacat aagtcccttg acggccttga
     1141 gtttgagttc ggctgaacgc agtgcccagt accagccttc gacgaggcat tccgggttgt
     1201 taaaaatagc gggcgacttc agggtaggca taggggtgtg gagtgcggcg gcttgacgcc
     1261 gcttttgtag aatcagcacc aaccttttag ctagtaaaac aaaaaatagc caacgtcaag
     1321 ggattgaact ctgaaaagca caaagcggca tcaagctgcc
//
LOCUS       DAOQDO010002082         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118027_length_1359_cov_2.371933, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002082 DAOQDO010000000
VERSION     DAOQDO010002082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118027_length_1359_cov_2.371933"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..914)
                     /locus_tag="PKZ53_25120"
     CDS             complement(255..914)
                     /locus_tag="PKZ53_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNJ43787.1"
                     /translation="MPISPADGGIKNYHNYFVPEEFTRRDPETQNLYLRDGQRAVYAS
                     EDFIVGLHSGLTQEVGDAANLIMYKCGYEWGVNDMKRFSERMRHEFGGGKLDVWQMNR
                     QFVMESWWWPLTVEGWGGWTIDFGFEKQSMVFITIRNSAVAKSMEQVGKPVCHMYAGM
                     FAGVFSVYEREERDAIEVQCYAMGNDSCKFLISKKDKVNAAEFWRREGANAQEILERL
                     A"
     gene            complement(981..>1359)
                     /locus_tag="PKZ53_25125"
     CDS             complement(981..>1359)
                     /locus_tag="PKZ53_25125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43788.1"
                     /translation="LSINPTHSLARKALAEMIFTDAIEAINIGDWDQGALLLKEAAEL
                     NPKHEPTWMWLASLTIETDPVQTIEYLKHVLDINPKNEKAVTWLDEIYQRILGSPVPP
                     TLIRQSETEVAPHHNTQDSLAVA"
BASE COUNT          316 a          344 c          363 g          336 t
ORIGIN      
        1 ggccaattgc cgaccaccgg cgatgttggg tggtactcaa agttgggtgc ataggttagg
       61 aaagtccaga gtcgtgtgga atgtcattga gggctgaaga aagcgggctt ggggctgagg
      121 gcgtgggggc tgaagacagc gggcttgggg ctgagggctg agggtaaaaa ccaattgaat
      181 tcaaatgatt tgtatccttt cagccccaag ccctcagccc caagccctca gccctggttg
      241 gttagccccg gtctttaggc cagtcgctcc aggatttcct gggcattggc tccttcacgt
      301 cgccaaaact cagccgcatt gaccttatct ttcttactga tcaggaattt gcagctatcg
      361 ttccccatgg cataacactg gacttcaatc gcgtcgcgtt cttcgcgttc atagacgcta
      421 aacacacccg caaacatacc ggcatacata tggcacacgg gtttcccaac ctgctccatt
      481 gatttggcaa cggctgaatt gcggatggta ataaaaacca tggattgttt ttcaaacccg
      541 aaatcaatgg tccagccgcc ccacccttca accgtaagcg gccaccacca ggattccatc
      601 acaaactggc ggttcatttg ccagacatca agcttgccac cgccaaattc gtgccgcata
      661 cgttctgaaa agcgcttcat gtcattgacg ccccattcat aaccgcactt gtacataatc
      721 aggttggcgg catctccgac ttcctgggtc aacccggagt gcaggccgac aataaagtct
      781 tctgaggcgt acactgcccg ctggccatca cgtaaataga ggttttgggt ctcaggatca
      841 cgccgggtaa attcttctgg aacaaagtag ttatggtaat ttttgatgcc accatctgct
      901 gggctgatcg gcatagatac acgttctcct tgcggattaa agagtagaaa aacgcaggtt
      961 caaggggggc tgacggagcc tcaggctacg gcaagtgaat cttgcgtgtt gtgatgaggg
     1021 gcaacttccg tttcagactg acgaatcagg gtcgggggga ctggggagcc aagaattcgc
     1081 tgataaatct cgtcgagcca ggtcacagct ttctcatttt tcggattaat gtcgagcaca
     1141 tgctttaaat actcgatggt ctggaccgga tcggtttcaa tggtgagaga ggccagccac
     1201 atccaggttg gctcgtgttt gggattgagt tcggcggctt ctttgagcaa cagcgctccc
     1261 tgatcccaat caccaatatt gattgcctcg atggcatcgg taaatatcat ttcggccagt
     1321 gctttgcggg ccagtgagtg agttggattg atgctgagc
//
LOCUS       DAOQDO010002083         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118152_length_1358_cov_3.980046, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002083 DAOQDO010000000
VERSION     DAOQDO010002083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118152_length_1358_cov_3.980046"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKZ53_25130"
     CDS             complement(<1..411)
                     /locus_tag="PKZ53_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43789.1"
                     /translation="MSPPLSDPSSAFGSSSDRLSELRSLQLSFDVRVTTCGMSLQGTT
                     HISNEDNFLVFHTDQHRRAEAGDTLAAPVGAKGVLLAVADGLGGNLAGEIASSLAVQA
                     LSEEWIIRHKQRPPEEAFLQAVEATNERVWSAWQN"
     gene            complement(533..1198)
                     /locus_tag="PKZ53_25135"
     CDS             complement(533..1198)
                     /locus_tag="PKZ53_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNJ43790.1"
                     /translation="MDFILTAVEVRVLGALIEKEFTTPDYYPLTLNALVNACNQLSNR
                     EPVVSYDDSTVLDALFNLRGKRCARQISGADHRVPKYAEIFTEEFALKMPEIAVMDVL
                     MLRGPQTPGELRSRGGRIYEFGSLAEVEETLQTLETRSPDPLIIRLPRQPGTKETRFA
                     HLLSGEIKIEKPEPASMTESVVSKHSAENERMAQLESEVAALRQELTDLKQQFLEFRK
                     QFE"
BASE COUNT          335 a          329 c          363 g          331 t
ORIGIN      
        1 attttgccag gctgaccaga cacgttcgtt ggtggcctca accgcctgaa gaaacgcttc
       61 ttcgggtgga cgttgtttgt gacggataat ccactcttct gaaagtgcct gcacagctaa
      121 gctgctggca atttcaccgg ccaggtttcc tcccaggcca tctgccaccg ccagaaggac
      181 gccttttgcc ccaaccggcg ccgccagcgt gtcgccagct tcggcccggc ggtgctgatc
      241 agtgtgaaac accaaaaaat tgtcttcgtt ggaaatatga gtggtgccct gaaggctcat
      301 cccgcaggtg gtaacccgaa catcaaaaga aagctggagc gaccggagtt cactgagtcg
      361 gtcggaactg gaaccaaatg ctgaactggg gtcgctaagg ggcgggctca taacagcaac
      421 cataaatgtg ttgatacctc tacttcttcg atagactttg aatctgaaga agcagcgtcg
      481 caacgggaaa accgaagaat gttccctggt tcctgagagc cacctgggac gttcattcga
      541 attgttttcg gaattcgagg aattgctgct tgagatcggt gagttcctgg cggagtgccg
      601 caacttcaga ttcaagttgc gccatacgtt cattttccgc tgagtgttta ctgacaacgc
      661 tttctgtcat cgaagccggt tcaggctttt cgatttttat ttctccgctg agcaggtggg
      721 caaaccgtgt ctctttggtt cctggctgac gtggtaaccg gatgatcaat ggatctgggc
      781 tgcgggtttc caacgtctgg agggtttctt caacttcggc cagtgagccg aattcataaa
      841 tccgaccgcc acggctccgt agttcgccag gagtttgagg cccccggagc atcaggacat
      901 ccatcacggc gatttccggc attttgaggg caaattcttc cgtgaaaatt tccgcatatt
      961 ttggcacccg gtggtcagca ccactgattt gacgggcaca tcgtttccct cgaaggttaa
     1021 acagggcatc cagaacggtt gaatcgtcat aggaaaccac tggttcacgg ttcgagagtt
     1081 gattacaggc gttcaccagt gcattgaggg tgagcgggta gtagtcaggc gtggtgaatt
     1141 ctttctcgat cagggcgcca aggactcgta cttcaacggc agtcaggatg aaatccacgg
     1201 caaactcgct tcctttgatt tgaagataaa ctctttctgg agaaatcaga aatgaggctg
     1261 gatcaacact tatgcaccaa gctacaccag atgtaaagcc atataagaat gaagaatgaa
     1321 gaatgaagaa tgaagaatga agaatgaaga atgaagaa
//
LOCUS       DAOQDO010002084         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118161_length_1358_cov_3.535687, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002084 DAOQDO010000000
VERSION     DAOQDO010002084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118161_length_1358_cov_3.535687"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..605
                     /gene="mscL"
                     /locus_tag="PKZ53_25140"
     CDS             168..605
                     /gene="mscL"
                     /locus_tag="PKZ53_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNJ43791.1"
                     /translation="MLKEFKEFAMRGNVLDMAIGIIIGGAFGKIVTSFVGDILMPPIS
                     KILGNIDFSNKFINLSDKPAATLAEAKAAGLVTINYGLFFNTVLDFLIVAFAIFLLVK
                     QVNRFKRQEEPAPPTTKDCKYCCSPIPVNAVRCPQCTSQLNAA"
     gene            820..1356
                     /locus_tag="PKZ53_25145"
     CDS             820..1356
                     /locus_tag="PKZ53_25145"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ43792.1"
                     /translation="MNQRKPFPTISPEDWAATPPAVRELLDHLVQIATEIELVRTRAA
                     SHDDPDLTHHEVHATMGNPIAKLVVLAPNRAMVYSFPIFKKEVFIGRIDRRSASEPDI
                     DLSEFDIKTRVSRRHARIFCEDNNFYIEDLGSVNGTVINDSRKLRPNTPYKLNDQDEI
                     RVGSSISLMLRTEPATVE"
BASE COUNT          347 a          363 c          288 g          360 t
ORIGIN      
        1 tgatctgaac gaaggtgccc tttccgtgct tatcacagtt caccaattct cacacttgac
       61 agtaaaacct gttccgtgga atggtttgac gcttcctttt cccttcaaac atcgaacttt
      121 cgtttgcccc caaagtgaac tgacactcat ctttcctgga gacatctatg ctcaaagagt
      181 ttaaagaatt tgccatgcgt ggaaatgtgc tcgatatggc catcgggatc atcatcggag
      241 gcgcgtttgg taaaatcgtg acctcgtttg tgggcgatat tttaatgcca ccgattagca
      301 agattttagg aaatattgat ttctcaaata aattcatcaa cctttcagat aaacccgccg
      361 ccacactggc cgaagccaag gctgcagggc tggtcaccat taactatggt ctgtttttta
      421 ataccgtgct tgatttcttg atcgttgcct ttgcgatttt tcttctggtc aaacaggtaa
      481 accggtttaa gcgacaggaa gaacccgcac ctcccacgac caaagactgt aagtactgct
      541 gctcacccat tccggtcaat gccgttcggt gtccacaatg tacgtcccag ttaaatgccg
      601 cgtgagtcaa atcattcaaa acctgggcac agcgttcact gtgcccaaat cgtttttcca
      661 acggcggaat ctgctttcag ccaggccata aaatgtggag ttgattcaac actcaaacat
      721 tgccaaacct gcctcatgtg ctgttattat ccatgtttcc atctgcacct gagcgtcggt
      781 tgcttgtcct cattcatatt ttaacaaagt gcgattttca tgaatcaacg gaagcctttt
      841 cccaccatta gtcctgaaga ttgggcagca acccccccgg cagttcgaga gttgctggac
      901 catctggttc agattgcaac cgaaatcgaa ctggtccgaa cccgggccgc ctcacacgat
      961 gatcctgacc tgacccatca cgaagtccat gccacgatgg gaaacccgat tgccaaactg
     1021 gtcgtcctgg caccaaaccg ggcgatggtg tacagttttc caatcttcaa aaaagaagtc
     1081 tttattggcc ggattgaccg ccgaagcgct tcggaacccg acattgattt atcggaattt
     1141 gacatcaaaa cccgtgtctc ccgccgtcat gcccgaattt tttgcgaaga taataacttc
     1201 tatattgaag atttgggcag cgttaatgga acggtcatca acgattcacg caaactccgc
     1261 cccaataccc cctataagct caacgaccag gatgaaatcc gggtcggcag cagcatttcg
     1321 ctgatgcttc gaaccgaacc agcgacggtt gaatagtc
//
LOCUS       DAOQDO010002085         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118170_length_1358_cov_3.060629, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002085 DAOQDO010000000
VERSION     DAOQDO010002085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118170_length_1358_cov_3.060629"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1257
                     /gene="ttcA"
                     /locus_tag="PKZ53_25150"
     CDS             376..1257
                     /gene="ttcA"
                     /locus_tag="PKZ53_25150"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNJ43793.1"
                     /translation="MDKLEKLQKTMFRRVGEAIKDFHMIQDGDKIMVCLSGGKDSFTM
                     LDMLLHFQKKAPIHFDLLAVNLDQKQPGFPEHILPEYLTQLGVPFVIVEQDTYSIVKR
                     LIPEGKTTCSLCSRLRRGILYNTAIEHGCTKIALGHHSDDIMETFFLNLFYVGTLKAM
                     PPILRSDDGRNTIIRPMAYCLEKKIAEYARERDFPIIPCDLCGSQENLKRKKVKNILK
                     QLENEIPRVKNSILAGFGRIQPSHMLDKTLYDFKNFEFLTGDVEAELDDALGFNHNEH
                     EMLHTDPDRLSISLPVL"
BASE COUNT          377 a          334 c          277 g          370 t
ORIGIN      
        1 gaagaaattg tatttccctg actcttgacc ccgagcctct gatcccaaag tggtattaga
       61 catgagttca acccaatctt ttgaataatt gtgccagagg atccagcctg tgattctctg
      121 gtactttttc tttcctgcag atactttatt atgtttttcc atattaactt ataaaatcaa
      181 aaacacgaaa aaccaaatac ccgattgtgt ttttcttgcc aaagaacccg gcttaaagtt
      241 gattttttcg agtagaccag gaaaaaccgg actcagtgct tgaattaaac aacaccggat
      301 tgctactttt cagggttgca atcagtgacg cactcatctc cttatcagtt ctcttcactt
      361 tgggcccttt agaacatgga caaactcgaa aaattacaga agacaatgtt tcgccgcgtt
      421 ggtgaagcca tcaaagattt tcatatgatc caggatggcg acaaaatcat ggtttgtttg
      481 agtggtggca aagacagttt taccatgctg gacatgctgc tccactttca gaagaaagca
      541 ccaatccact ttgacctgct cgccgtcaac ctcgatcaga aacaacctgg atttcctgaa
      601 cacatcttgc ctgaatatct gacccaactg ggcgttccgt ttgtcatcgt tgaacaggat
      661 acctactcca ttgtcaaacg tctcattcct gaagggaaaa cgacctgctc gctctgttca
      721 cgcctgcgac ggggaattct ctacaacacc gccatcgagc acggctgtac caaaattgcc
      781 ctgggtcacc actccgacga tattatggag acgtttttcc tgaatctgtt ttatgtcgga
      841 acgctcaaag ccatgcctcc aattttgcga agcgacgatg gccgcaacac gattattcga
      901 cccatggctt attgtttgga gaagaaaatc gctgaatacg cccgcgaacg ggatttccca
      961 atcattccct gtgatttgtg tggctcgcag gaaaacctga aacgcaagaa ggtcaaaaac
     1021 atcctcaaac aactggaaaa tgaaattcca cgggtcaaaa actcgattct ggctggcttt
     1081 ggtcgcatcc agccgtcgca tatgctggat aagacgctct atgactttaa aaattttgaa
     1141 ttcctgactg gcgatgttga agccgaactc gatgacgcgt tggggtttaa tcacaatgaa
     1201 cacgagatgc tccacaccga cccggatcga ttgagcattt ccctgccggt cctgtaaaac
     1261 attagagctt ggggcttggg gcttgaaaat tcaatcgttt aaacctacgc aaatcccaag
     1321 ctttcattcc aaacagagct gatagacttc ctgaattt
//
LOCUS       DAOQDO010002086         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118174_length_1358_cov_2.972371, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002086 DAOQDO010000000
VERSION     DAOQDO010002086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118174_length_1358_cov_2.972371"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..588
                     /locus_tag="PKZ53_25155"
     CDS             121..588
                     /locus_tag="PKZ53_25155"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNJ43794.1"
                     /translation="MSKLPESVAAFLSHHAIAVAGVSRDSSQPANAVFKRLKECGHLV
                     FAVNPKTEQVENGPCYPNLRSLPQPVGAVVIATHPNVSAQVVRDCAELGIRHVWFHRS
                     FGEGSVSEEAIRECQANGIECIVGGCPMMYCGHVDFGHRCMKWILKFQHRVPG"
     gene            737..1171
                     /locus_tag="PKZ53_25160"
     CDS             737..1171
                     /locus_tag="PKZ53_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNJ43795.1"
                     /translation="MNWQDLSATSPYQTALEIEDELAKGNIAEASAGLKELIEALGRS
                     EKRALKSQLIRLMAHIIKWKTQTQKRSRSWVASIYNAREEIADIQEETPSLTNEVIEE
                     IWEKCFQAAKRDAEAEMNQDTRMAGLSWEDVFEAEYKNESSE"
BASE COUNT          388 a          300 c          346 g          324 t
ORIGIN      
        1 ttgacgaagc gaccgggctg attgcccggc gatacgctcc ggagcgctat caggaaaaag
       61 attttggttc aaagttcaag ccttaactta cccaaactgt aatgcgacag gcgaaatttt
      121 atgtcaaaac ttcctgaatc agttgctgcc tttcttagcc accacgcgat agccgttgca
      181 ggtgtctccc gagattccag tcagcctgca aatgctgttt tcaaaaggct taaagaatgc
      241 ggtcatcttg tttttgccgt caatccaaag actgaacagg tggaaaatgg gccgtgttat
      301 cccaatctcc gctcacttcc tcagccagtt ggggccgtcg tcattgcaac ccatccgaac
      361 gtctcagccc aggtcgtgcg tgactgcgcc gagctgggaa tccggcatgt ctggtttcat
      421 cggtcatttg gagagggaag cgtgtctgaa gaagccattc gcgaatgtca ggcaaatggg
      481 attgagtgta tcgtcggagg ttgcccgatg atgtattgtg gtcacgtaga ttttggccat
      541 cggtgtatga agtggatcct caagtttcaa catcgtgttc caggatgaat ggcagaatca
      601 gagttgatgg taaataatgc agagtccata ctgggagcgt taatcccatc gtgaaccgtg
      661 agtcacccca tgacccgaaa tggtataagt gcttatggct gctcaaaaat ggcttcaagc
      721 aataaggaga tcaggcatga actggcaaga tttatcggcc acctcgccct atcaaacagc
      781 cttggaaatt gaagacgaac tggccaaagg aaacattgct gaagcctcag ccggcttaaa
      841 ggagttaatc gaagccttgg gacgatcaga aaaacgcgcc ttaaaaagtc aactcattcg
      901 gttgatggcc cacatcatca aatggaaaac acagacgcaa aaacgatcac gcagttgggt
      961 ggcttcgatt tacaacgctc gcgaagaaat cgccgatatt caggaagaaa ccccaagttt
     1021 gaccaatgag gtcatcgaag aaatctggga aaaatgcttc caggcagcaa aaagagacgc
     1081 tgaagcggaa atgaaccagg acacgaggat ggctggtctt tcgtgggaag atgtgttcga
     1141 ggcagagtat aaaaatgaat caagcgaatg actttttcta tactatgaat aacactgagg
     1201 tacaggtatg gaattaacaa ttactgttcc agaagattct aaactcgctt tagagcaaag
     1261 ggcacgcgaa cgcggattca cggatatatc caggtatgtt gagcgcttga ttgaaactga
     1321 ccttttggcg gcaaggtctt ttgatgaaat cttgtccc
//
LOCUS       DAOQDO010002087         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118188_length_1358_cov_2.787414, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002087 DAOQDO010000000
VERSION     DAOQDO010002087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118188_length_1358_cov_2.787414"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1358
                     /locus_tag="PKZ53_25165"
     CDS             <1..>1358
                     /locus_tag="PKZ53_25165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknK"
                     /protein_id="HNJ43796.1"
                     /translation="HPDHPLVLFLDDLQWADSDSLKLLQMLFTVNPIPNLLMISAFRA
                     NEVSTAHPLTLVLEEIHKSDTTVTELQLRALDQDTVTRFVADALHSDPSVVGGLAELI
                     YKKTAGNPFFTGEFLKSLTQNDLIGFDAQLGRWIWDLEQITQTQITDNVADLLAGKLK
                     KLAPSTQEIVAAAACLGSQFSFHYLCLVTQKSPALVLADIRDAINEELLLPIGDGYRL
                     LEFSAGDTQLDATFECCFVHDRIQQAAHSLLDDAQKTALHLVIGRRLEHGLSASEREQ
                     EQFTIVNHLNLGGDLLANEVERTDLARKNLAAGRKAKASAAFQPAYDYFETGLRFLPP
                     SAWETCYELTLALHEEAAETALWCAQTERADEFIAIVLANVRRFIDSIRVRDAQIQSL
                     ISKNRLQDCIACSLEALNQLGLVIPSSPTEADVQGVFMEVQQLLAGRPPFVGEASSMV
                     IA"
BASE COUNT          304 a          357 c          350 g          347 t
ORIGIN      
        1 caccctgatc atcccctggt gctctttctg gatgaccttc agtgggcaga ctctgattcg
       61 ctgaaactgc tccagatgct gtttaccgtt aacccaattc caaacctctt aatgatcagt
      121 gcttttcggg ccaatgaggt ttcgactgct catccgttga cgctggtgct tgaagaaatt
      181 cacaaatcag acacaaccgt gaccgaactt caactcagag cccttgatca agacactgtt
      241 acccggtttg tggcggatgc acttcattca gatccatcag tggttggcgg cctggcggag
      301 ttgatttata agaagacagc gggaaatccc tttttcaccg gggaatttct gaaatcgctg
      361 acccaaaacg acctgattgg gtttgatgct cagcttgggc gctggatctg ggatctggag
      421 cagattaccc agactcagat aaccgacaat gtcgcagatt tgctggctgg caaattgaaa
      481 aaactggcgc cgtcaacgca ggaaattgta gcggcagcag cctgtcttgg tagtcaattt
      541 tcatttcatt atctgtgtct ggtgacccaa aagtctccgg cactggttct ggctgatatt
      601 cgggatgcca tcaacgaaga actcctcttg ccgattggag atgggtaccg gttgctggaa
      661 ttttctgctg gcgataccca actggatgcc acctttgagt gctgttttgt ccacgaccgg
      721 attcaacagg ctgcccactc gctgctggat gacgcccaaa aaaccgctct tcatctggtg
      781 attggacgtc ggttggaaca cggtctttcc gcctccgaac gggaacagga acaatttacc
      841 attgtcaatc acctgaattt gggcggtgat ttgctcgcca acgaagttga acgaactgac
      901 ctggcacgca aaaacctggc agccgggcgc aaagccaaag cctcggcagc cttccagcca
      961 gcctatgatt attttgaaac tggtttgcgc tttctccccc cgtcggcctg ggaaacgtgc
     1021 tatgaattaa ccctggcctt gcacgaagaa gcagcggaaa ccgcgctctg gtgtgcccaa
     1081 accgaacgag ctgatgagtt tattgccatt gtgctggcca atgtccgccg tttcattgat
     1141 tcgatccggg tgcgtgatgc tcagattcaa agcttgattt cgaaaaatcg tcttcaggac
     1201 tgtattgcct gctcactgga agcactcaat caacttggcc tggtgattcc ttcgtcgccg
     1261 acggaagccg atgtccaggg tgtttttatg gaagtccagc aactgctggc cgggcggcca
     1321 ccgttcgttg gcgaggcgtc gtccatggtg atcgcgca
//
LOCUS       DAOQDO010002088         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118211_length_1358_cov_2.451266, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002088 DAOQDO010000000
VERSION     DAOQDO010002088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118211_length_1358_cov_2.451266"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..>1358)
                     /locus_tag="PKZ53_25170"
     CDS             complement(376..>1358)
                     /locus_tag="PKZ53_25170"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNJ43797.1"
                     /translation="LHSQFFILNSSFSILHSQFFMRIWLSKHTEVPIREQLTEQVMFG
                     IISQELQPGQRLPSIRELARRLRIHSNTVNAAYHELARRGWVEFRKGSGVYVRTLAAE
                     SRETGSQAELDQHIASFLRMLHRRGFSQAAIQSRLHYLLELQPPDHFLILESDPHLQQ
                     ILKQELTDATQFPVLVTPLETVKSTPQVLIGAIPVALYSRAELVRPLLPPQTECTWLQ
                     LRSIPKSLTAYQKPGEEALLSVVSHWPEFLKWGRMLLIAAGIAPDAVHCVTPTDPDWV
                     RSLRHSYLVITDVVTAQLLPAGTPIAIYRVIGDESFAELQHMVASLISGE"
BASE COUNT          384 a          323 c          367 g          284 t
ORIGIN      
        1 cagtgaaaaa accactccgc aactgatcac aagaaaccag cgtgaaaaaa tcataagcaa
       61 ctcctgggaa aaattctggg aacctgatga atagcggcca atagcggcct gaaaggaaga
      121 tggtttgagc acgatctcaa tcaagcagtc tctgaattca cttgagagaa acaaaccagc
      181 tttccgcacc gcgacgatat cgcgtcgata caaccgaaac agcaaatgag agagttgatt
      241 gggatcgtgt tgcggagaaa ataacccggc tggggctcac acgagattgg ctctatcaca
      301 ctttttctga ctgtgacaat tccgggtgac gaactggaac cggtcactgg aaacgagaat
      361 aggtgggaac cagggttact cgcccgagat cagactggca accatgtgct ggagttcagc
      421 aaaagactcg tctccgataa ctcgataaat ggcaatcggc gttcctgctg gcagcaattg
      481 tgccgtgacc acatcggtaa tcaccagata actatgccgg aggctgcgca cccaatcagg
      541 gtcagtcgga gtcacacaat gcacggcatc tggtgcaatt ccagcagcaa tcagcagcat
      601 tcgcccccat ttcagaaact ccggccagtg tgacacaact gacaacagcg cctcttcacc
      661 tggtttctgg taggcagtca gcgatttcgg aatggatcga agctggagcc aggtgcattc
      721 ggtttgcggc ggaagtaatg gtcggaccaa ctcagcccgg ctatagagtg cgaccggaat
      781 ggcaccaatc aacacctgag gcgttgactt taccgtttca agcggagtga ctaaaaccgg
      841 aaactgtgtg gcatcagtca gttcctgttt gagaatttgc tgcaaatgcg ggtcagattc
      901 gaggatgaga aaatgatctg gtggctggag ttcaagcaga tagtggaggc gggattgaat
      961 cgcggcctgt gaaaagccac gtcggtggag cattcgcaaa aatgaggcaa tatgctggtc
     1021 aagctctgct tgtgaaccag tttcgcggga ttcagcagcg agcgttcgga catagacgcc
     1081 gcttcccttg cgaaattcaa cccatccacg acgggccagt tcatgatatg ccgcattaac
     1141 cgtgttggaa tgaatgcgca accggcgggc cagttccctg atgctgggca atcgctggcc
     1201 gggctgcaat tcctgactga tgatcccaaa cataacctgt tcagtcagtt gctcgcgaat
     1261 tggcacctca gtgtgctttg aaagccagat acgcataaag aactgagaat gaagaattga
     1321 gaatgaagaa ttgagaatga agaattgaga atgaagaa
//
LOCUS       DAOQDO010002089         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118239_length_1358_cov_2.248657, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002089 DAOQDO010000000
VERSION     DAOQDO010002089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118239_length_1358_cov_2.248657"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1358
                     /locus_tag="PKZ53_25175"
     CDS             <1..>1358
                     /locus_tag="PKZ53_25175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43798.1"
                     /translation="TILILVWSLVEHGRGIVHSRSMGALLMGSGILALPCSVYLWGHY
                     SELSSEWHPETLYFRGFGISNIFKNLAREHLRYDQWESGVLKIATEDFWSVSLFQLLI
                     GLSLLYLGVRVVVAFTRSHGWQEFTEMLQSMTPEGQALAQDRSQFFHLGPDEPRYTGS
                     LLRSMARQGGWRRKLTVALHELEEESRPVTTWLDALGAPSHPAWRASRAGLLLVSLVS
                     IIFFALFDAQKSAFHLPFITVWLALCCGVGIVDGIRWGIEQRKRSAKRVLMGLGALLL
                     VVIPTIGYATSASRVVWRFYRWSRTFEPAPYSDVTQVLRTIVPQNLLVVSRPVHWLSF
                     QDNPNYVWASKKVWKDQDITSLPGVLVVDDEFIERENYNRLFARQVPNFTLLAELSDT
                     LYGNIKVYYTGLDGRFQNHTPAHYYFMGGYHKGYARKGYYSEEQRQAAKVIWLAGPPE
                     LE"
BASE COUNT          272 a          323 c          408 g          355 t
ORIGIN      
        1 cacgattttg attctggttt ggtcactggt tgagcacggg cgggggatcg ttcacagtcg
       61 gtcaatgggg gcgttgctca tggggtcggg gatcctggca ctcccctgca gtgtgtattt
      121 atggggccat tattctgagc tttcatctga gtggcatccg gagaccctgt attttcgagg
      181 ctttgggatc tctaatattt ttaagaacct ggcccgcgag cacctgcgat atgaccagtg
      241 ggaaagtggc gtcttaaaaa ttgcaactga ggatttctgg tcggtcagcc tgtttcaact
      301 gttgattgga ttgagtttgt tgtatctcgg agttcgggtc gtggtggcat ttacccgctc
      361 tcacggatgg caggaattta ccgagatgtt gcagagtatg acgcctgaag gtcaggccct
      421 ggctcaggat cggtcacagt tttttcattt aggacctgat gagcctcgat acaccgggtc
      481 attgcttcgc tcaatggcgc gccagggcgg gtggcgacga aaattaaccg ttgcactcca
      541 cgaattggaa gaggaatccc gtccagtgac aacctggcta gacgcgctgg gggcgccatc
      601 tcaccctgcc tggcgggcct cgcgtgccgg gctgttgctg gtttcactgg tcagtatcat
      661 cttttttgcc ctgtttgacg ctcaaaaaag cgcgtttcac ctgcccttta tcactgtgtg
      721 gctggccttg tgttgtggtg tcgggattgt agacggtatt cgatggggaa ttgagcagcg
      781 gaaacgaagt gcaaaacggg tactgatggg ccttggggca ctcctgctgg tggtgattcc
      841 gacgattgga tatgcgacct cggcttcccg cgtggtgtgg cggttctatc ggtggtcgcg
      901 aacctttgag ccggcgccct actcagatgt cacccaggtt ttgcgcacga ttgtgcctca
      961 gaatttactg gtggtgagtc gcccggttca ctggctgtca tttcaagata atccgaatta
     1021 tgtctgggcc agcaaaaagg tctggaaaga tcaggatatc accagtctgc ctggggtgct
     1081 ggtggtggat gacgagttta ttgagcggga aaactataac cgcctctttg ccaggcaggt
     1141 tccgaatttc acgttgctgg ctgaactgag tgacacgctg tacgggaata tcaaggtcta
     1201 ttacacgggg ctggacggtc ggtttcaaaa ccacacacct gcccactact actttatggg
     1261 aggctaccat aaaggatatg cccgcaaagg atattacagc gaagaacagc gtcaggccgc
     1321 caaagtcatt tggctggccg ggccacccga actggagc
//
LOCUS       DAOQDO010002090         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118393_length_1357_cov_2.333333, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002090 DAOQDO010000000
VERSION     DAOQDO010002090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118393_length_1357_cov_2.333333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1357)
                     /locus_tag="PKZ53_25180"
     CDS             complement(<1..>1357)
                     /locus_tag="PKZ53_25180"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNJ43799.1"
                     /translation="TSLQFALVSLIVAIFVFPAMGQTHPTYSVPPSPPLTGWQPAAAG
                     PARSITESTHASPPDLLTHFEKSNYVETGRYAEALELCRKLEKRSPLVKVQTIGTTPQ
                     GRDLVVVIVSRDRAFTPEAAAKTGKPVIFIQNGIHSGEIGGKDASLMFLRDVVVTKKY
                     AALLDHVILLVMPVFNIDGHERVSPYNRINQAGPREMGFRVTAQRKNLNRDYLKADTP
                     EMRAWLRMWTSWNPDFLMDNHVTDGMDYQYDVTISIPNGAEVWPTVGTWIRNSFLPKF
                     QAGLEQDGHVVGPYIEPIDEQDVKKGFQGGPSTPRFSTGYAAICSRPGLLVETHSLKP
                     YRTQVWSHYDVIVQTLQFIAENSSSLKQAVTQADEQIAALSQKTPGSQLYLSGKLGTA
                     SEPYAFRGVTTTFEPSPITGSTLPRYGNQPVTVDSVFYDQTIPTASPVVPYGYLIPAE
                     WT"
BASE COUNT          325 a          322 c          387 g          323 t
ORIGIN      
        1 gtccactcag ccgggattaa atatccatag ggaaccacgg gtgaagctgt gggaatggtc
       61 tgatcataaa aaactgaatc tacggtgacg ggctggttgc catagcgtgg gagggtgctg
      121 cctgtaattg ggctgggctc aaacgtggtg gtcacgccgc gaaaagcata tggctcgctg
      181 gcggtcccaa gcttgcctga taaatataat tgactccctg gggttttttg cgagagggcc
      241 gcaatctgtt catctgcctg ggtcactgcc tgcttgagag aggatgaatt ttcagcaatg
      301 aactgcagcg tttgaacaat cacgtcatag tgtgaccaga cctgagtccg atagggtttg
      361 agactatgcg tttcaaccag taaccctggg cgactgcaaa tggccgcata cccggtcgag
      421 aatcttgggg ttgaggggcc accttgaaac ccttttttga cgtcctgttc gtcaattggt
      481 tcgatatagg gcccaacgac gtgcccatct tgttcaagtc ccgcctgaaa tttcggcaaa
      541 aaggaatttc gaatccaggt gccaaccgtt ggccagactt cggctccatt gggaatactg
      601 atggtgacgt catattgata atccatgcca tcggtcacgt ggttatccat caagaagtcg
      661 gggttccaac tggtccacat tcgaagccag gcgcgcatct ccggtgtatc agccttgaga
      721 tagtcacgat tgagattttt gcgctgtgcg gtgacgcgaa atcccatttc acgcgggccg
      781 gcctggttaa tgcggttata tggcgaaaca cgttcgtgac cgtcaatatt aaaaactggc
      841 atcacaagca aaataacgtg atctaacaga gccgcatatt ttttggtgac gacgacatcg
      901 cgcaaaaaca tcagtgaggc atctttgccg ccaatctcac ctgaatgaat tccattttga
      961 ataaagatga caggtttgcc ggttttcgct gcggcttcag gggtaaaagc ccgatccctg
     1021 gaaacgataa ccaccaccag gtcacggccc tggggggtgg tgccgatggt ctggactttg
     1081 acaagcggag atcgtttttc aagtttccga cacagttcca gggcctcagc atagcgccca
     1141 gtctcaacat agttggattt ttcaaaatgg gtcagcaaat caggcggcga ggcgtgtgtg
     1201 ctctctgtga ttgaacgggc aggtccggct gcggctggtt gccagccagt cagggggggc
     1261 gaaggcggaa ccgaataggt tggatgggtt tgacccatgg ctgggaaaac aaaaatggcg
     1321 acaatcaggc tgaccagcgc aaactggagg ctggtgc
//
LOCUS       DAOQDO010002091         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118519_length_1356_cov_2.974635, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002091 DAOQDO010000000
VERSION     DAOQDO010002091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118519_length_1356_cov_2.974635"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1174
                     /locus_tag="PKZ53_25185"
     CDS             221..1174
                     /locus_tag="PKZ53_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNJ43800.1"
                     /translation="MPSDHLVVGIAQMSPVWLNRQATTEKMLAFLENAAEQRCQLVVF
                     SEALLPGYPFWIERTDGAQFNSPIQKEIHAHYLDQAVQIESGHLSALCQAAKCHSLAV
                     VLGCVERPLDRGGHSLYCSLVFIDPKGEIRSVHRKLMPTYEERLTWSPGDGHGLHVHQ
                     LEAFTVGGLNCWENWMPLARAALYGLGEDLHIAVWPGGIRNTENITRFIALESRSFVI
                     SASGLMRPTDFPSETPHRDLIVQNCEPFLANGGSCIAAPDGTWLLSPVIEDETLLVAR
                     LDHTLVRQARQNFDPAGHYSRPDVTHLTVNRKRQSTVSFTD"
BASE COUNT          302 a          357 c          331 g          366 t
ORIGIN      
        1 gttccgggtt ccgggttccg ggttccgggt tccgggttcc gggttttcga agaggaaaag
       61 cttttgtcac agaagtttat cgtgattgtg aatggaacca attgaaactt gaaaccggtt
      121 cacactacag ttgatttgac ttttgacatt ccctttttgg ccaccagccg actgtaaaca
      181 atctgtattc aaaaaccccg aacccggaac tcctacccac atgccttccg atcaccttgt
      241 tgtcggtatt gcccagatgt ctcctgtctg gctcaatcgt caggccacga ccgaaaaaat
      301 gctggccttt cttgaaaatg cggctgagca gaggtgtcaa ctggtggtgt ttagtgaagc
      361 cctcctgcct ggatatccct tttggattga gcgaaccgac ggtgcccagt ttaattcacc
      421 gattcaaaaa gaaatccacg cccactatct tgatcaggcg gtgcaaattg agtctggtca
      481 tctgagtgcc ctttgccagg ctgctaaatg tcattctctg gcggtggttc ttgggtgtgt
      541 tgagcgcccg ctggatcgtg gtggccacag cctctactgc tcgctggtgt ttattgaccc
      601 gaaaggtgaa attcggtcag ttcatcgcaa gttaatgcca acctatgaag aacgcctcac
      661 ctggtcacca ggagatggac acgggttgca cgtgcatcaa cttgaggcct ttaccgtagg
      721 tgggctcaat tgctgggaaa actggatgcc cctggcccgt gcggcgctct atggcctggg
      781 ggaagatctc catattgcag tttggcccgg aggaatccgc aacaccgaga atatcacccg
      841 atttatcgcc ctggagtccc gctcatttgt gatttctgcc tctggattga tgcgccccac
      901 cgattttccc tctgaaactc cccatcgtga cttgattgtc cagaattgcg aacccttcct
      961 ggccaatggc ggctcctgta ttgccgcacc ggatggcacc tggctgcttt caccggtcat
     1021 tgaagatgaa acgcttctgg tggccaggct cgaccacaca ctggttcgac aggcacggca
     1081 aaactttgat ccggctggac attattcccg ccctgatgtc acccatctca ctgtcaatcg
     1141 caagcggcaa tccaccgttt catttacaga ttaaattcgg aaaaattttt aaacgactgt
     1201 cgattctggg aaaactgatt cgtcattgga tcaaacacgc cttctcgata cacttcgaag
     1261 ggcaaatttt gtcaatgatt aggagaaatg attgctatgc ctcagtttat gttgatttcc
     1321 atgtatgata tgtccgaagc cgccgatgtc gaactc
//
LOCUS       DAOQDO010002092         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118528_length_1356_cov_2.835511, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002092 DAOQDO010000000
VERSION     DAOQDO010002092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118528_length_1356_cov_2.835511"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1356
                     /locus_tag="PKZ53_25190"
     CDS             71..>1356
                     /locus_tag="PKZ53_25190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43801.1"
                     /translation="MTPERYQKLTELFQAALDQPVELRAAFLVQACGDDHDLLAQVEK
                     LLARHEEIGDLIAPPVLEAGMKAVSTAVIEQEAKSMIGRHIGIYRLVKELGRGGMGVV
                     YQATRTDDHYKPVAIKILKRGMDTDSILSRFYRERRILAGLDHPHIARLLDGGTTSDG
                     LPYFVMEYIDGQPIHTYCDTRRLSITERLNLFRTVCSAIQYAHQNLVVHRDLKPGNIL
                     VTTDGTPKLLDFGIAKVIKSDETTQEDLTQVDLTQPGQRPMTPAYASPEQIRGEAVTT
                     ATDVYCLGILLYELLTGQLPYHLSPSNPDETLKIICTQKPERPSTLINRRPKVFPKAA
                     SLQATEAASIVREGTPDRLRRRLAGDLDNIVLMALRKDPRRRYSSVEQFSDDIRRHLE
                     GRPVSARQDTFGYRAGKFIQRNKLGVGVAAALLRHLV"
BASE COUNT          324 a          399 c          353 g          280 t
ORIGIN      
        1 aatatttcac cgcgaacagt caaacgcgag tggagcatgg cccgagcctg gctgctcaat
       61 gaattaagcc atgacgcctg aacgctacca aaaactcaca gaactgtttc aagctgccct
      121 tgaccaacca gtcgaattac gggcagcttt cctggtccag gcgtgcggtg atgatcacga
      181 cctgttggcc caggtcgaga aactcctcgc tcgccacgaa gaaattggtg acctgattgc
      241 ccccccggtg ctggaagccg gaatgaaagc tgtctccaca gcagtgattg agcaggaagc
      301 aaaaagcatg atcgggcgac atatcggaat ctatcgcctc gtcaaggaac ttggtcgtgg
      361 aggaatgggc gttgtttacc aggcaacccg gactgacgat cattacaaac cagtcgccat
      421 taagatttta aagcgcggga tggatacaga ttccattctg agccgctttt accgcgaacg
      481 ccggattttg gccgggttgg atcatccaca cattgcccgg ttgcttgatg gcggaacaac
      541 atcggacggg ttgccctact ttgtgatgga atacattgat gggcagccaa tccataccta
      601 ctgcgacacc cgccggcttt cgatcaccga gcgactcaac ctgtttcgca ctgtctgttc
      661 agccattcag tatgcccacc aaaatctggt ggttcaccgg gatctcaaac ctggaaatat
      721 cctggtgacc accgatggca ccccgaaact gctggatttt gggattgcca aagtcatcaa
      781 atctgacgaa accactcagg aagacctgac ccaggttgat ttaacccagc ccggtcaacg
      841 cccgatgacc ccggcctatg ccagcccgga gcaaattcgg ggcgaagccg tcaccaccgc
      901 cactgacgtc tactgcctgg gaatcttgct ctatgaattg ttaaccggcc agcttcccta
      961 tcatctgagc cccagcaatc cggatgagac gctcaagatc atctgcaccc aaaaacccga
     1021 gcgaccaagc acactgatca accgtcgccc gaaagtattc cctaaagcgg ccagtcttca
     1081 ggctaccgaa gcagccagca tcgtgcgcga aggcacccca gacaggctcc gtcggcgatt
     1141 ggctggcgat ttggacaata ttgttttgat ggccctgcgc aaggacccgc gccggcgcta
     1201 tagttcggtt gaacagtttt cagacgacat tcgccgccat ctggaagggc gcccggtgag
     1261 tgcccggcag gatacgtttg gctatcgcgc cgggaaattc atccagcgca ataaacttgg
     1321 cgtcggggtc gcggcggcac tgctccgcca tctggt
//
LOCUS       DAOQDO010002093         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118531_length_1356_cov_2.817064, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002093 DAOQDO010000000
VERSION     DAOQDO010002093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118531_length_1356_cov_2.817064"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKZ53_25195"
     CDS             <1..453
                     /locus_tag="PKZ53_25195"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HNJ43802.1"
                     /translation="SMGAVVGEKITRAIERAIAKRCPIVITSASGGARMMEGTLSLMQ
                     MAKISAALARLDDAKLPFISILTDPTTGGVTASYAMLGDLNIAEPGALIGFAGPRVIE
                     QTIRQKLPPGFQTSEFLLDHGMLDAVVDRVELRNYIIQALEFMTPAAR"
     gene            619..>1356
                     /locus_tag="PKZ53_25200"
     CDS             619..>1356
                     /locus_tag="PKZ53_25200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNJ43803.1"
                     /translation="MTYHETLDYLYALGHEVLAMKLGLENITHLLSALGNPHRTCRTV
                     HVAGTNGKGSTCAMLASILQTAGLRVGLYTSPHLERIEERIQINRQLIETREFTRYIK
                     EVRNTAEQLLTAGQLQARPTFFEHVTAAAFLYFHHQKPDVVVLETGLGGRLDATNVVS
                     PELVILTNIGHDHQQYLGNTLEAIAGEKAAIIKPDTPVLVAGTQPVEAAAVIRNQATA
                     VKAPLEWVSPLPILGRTATGELLVKVGQ"
BASE COUNT          317 a          359 c          376 g          304 t
ORIGIN      
        1 agcatggggg cggttgtcgg tgagaaaatc acccgagcga ttgaacgggc aattgccaaa
       61 cggtgtccga ttgtgattac ttcagcatcg ggcggtgccc gaatgatgga aggcaccctc
      121 agcctgatgc aaatggccaa aatcagtgct gcactggcca ggctcgatga tgctaaattg
      181 ccgtttattt caatcttgac tgatccgaca accgggggcg tgacggcgag ctatgcgatg
      241 ctcggtgatt taaatattgc tgaaccaggc gcgttgattg gatttgccgg accgcgagtg
      301 attgaacaaa ccattcgcca aaaattgccg ccggggtttc aaacctccga attcttgctt
      361 gaccatggga tgctggatgc cgtcgttgat cgggttgaat tgcgaaatta tatcattcag
      421 gcgcttgaat ttatgacgcc agccgcgagg tgagggctga gaaaaccagg gcttggggct
      481 cagggcttgg ggctgagaaa accagggctc agggtatgaa acctgttgtc ttcagccccg
      541 agcttgcgaa tcttcagccc tcagccccta gccctcagcc ctggtatttt cagcccaaaa
      601 tcttcagccc taaatggaat gacctaccac gaaacccttg attatctgta tgcactcggc
      661 cacgaagtct tagccatgaa gcttggcttg gaaaatatca cccaccttct ttccgcactt
      721 ggaaaccccc atcggacttg tcgaaccgtg catgtggcgg gaacgaacgg caagggctcg
      781 acctgtgcca tgctggcttc cattctgcaa acggctggtt tgcgcgttgg tctctacact
      841 tcgccccatt tggaacgcat tgaagagcgg atccagatca accgccagct catcgaaact
      901 cgcgaattta cccgatacat taaggaagtc cggaacacgg cggaacagtt actgactgcc
      961 gggcagcttc aggcacggcc aacatttttt gaacacgtaa cagcggcagc ttttctctat
     1021 tttcatcatc aaaagccaga tgtggtggtt ctggaaaccg ggttaggtgg ccggctggat
     1081 gccaccaatg tggtgtcacc ggaactggtc atcctcacca acattgggca cgaccatcag
     1141 caatatctcg ggaacacgct ggaggcgatt gctggtgaaa aagccgcgat tatcaagcct
     1201 gacacaccgg tgctggtggc cgggactcag ccagttgaag ccgccgctgt tattcgcaat
     1261 caggccacag cggtgaaagc gccactggaa tgggtatcgc cgctcccgat cctggggcga
     1321 accgcgacag gtgaattgct ggtcaaggtc ggtcag
//
LOCUS       DAOQDO010002094         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118689_length_1355_cov_3.219231, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002094 DAOQDO010000000
VERSION     DAOQDO010002094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118689_length_1355_cov_3.219231"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PKZ53_25205"
     CDS             <1..1161
                     /locus_tag="PKZ53_25205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ43804.1"
                     /translation="FEEPAQIEAIETEPGTRNLEPGFEAVNPSPLPSLPSPIAHPPSS
                     LPEFPRFSETLKLEPVKPVDAPIEEGLSAIESILQRGKTAKLKPIVPAEGVYSEYVHR
                     IQTEQGDISEAVRAITAPLTLTPQSSQWAEEVAPASESVATGALGRTSEVNVSAPPSA
                     ISSETRSATHVSSMSWEASHPINPLHESRLSPEPQPVWTVSFDELESGSLKPADMKEE
                     LAGKVAELMTAQELNKTLLAHWNNRFTQVEVSFIKLVVDPFVKYGTAVTPELSKAILS
                     PVADMIETECRTEPKLIAYCGSAEFFIVLPGVTSGEIAEIAERLRSQAESLWLSMPNR
                     ESDEWLTVSLGVVTSFPLKSSLDSLLSAVNLALELAKDAGGNRVMISPSPLD"
BASE COUNT          331 a          356 c          338 g          330 t
ORIGIN      
        1 tttgaagaac ctgctcaaat tgaagcgatt gagacggaac ccggaacccg gaacctggaa
       61 cccggttttg aagcggttaa tccatcccct ctcccctctc tcccatcccc tatcgcccat
      121 cccccatcct ctctccctga gtttccccgg ttttcagaaa cgctcaagct tgagccagta
      181 aagccggttg acgcaccaat tgaagaagga ctgtcggcga ttgaatcaat tctccaacgt
      241 ggtaaaactg ccaaactcaa gccaattgtt cctgctgagg gggtgtatag cgaatatgtc
      301 caccgaattc agactgagca gggagatatt tcggaggcgg ttcgagcaat aactgcgcca
      361 ctcaccctga caccgcaatc ttcacagtgg gcagaagaag ttgctccagc gtctgagtca
      421 gttgcaacgg gggcgttggg caggaccagt gaggtaaatg taagtgcccc gccatcagcc
      481 atttcatctg aaacaagatc tgcaacccac gtctcatcaa tgagttggga ggcttctcac
      541 ccgatcaatc ccttgcacga atcccgcctg tcacctgaac cccagccagt ctggactgtc
      601 tcatttgacg aattagaatc tggcagtctg aaaccggcag atatgaagga ggaactagcc
      661 ggcaaagttg ccgaattgat gacagcccag gaactgaata aaacgcttct ggctcactgg
      721 aacaaccgtt ttacccaggt cgaagtctct ttcatcaaac ttgtggttga tccatttgtg
      781 aaatatggga ctgcggtaac tcctgaactc agtaaagcca ttttaagtcc cgtagctgac
      841 atgattgaaa ctgagtgtcg cacagagccg aaactgattg cctattgtgg ttcggctgag
      901 tttttcattg tgttgccggg tgtgacttca ggtgagattg ctgaaattgc tgagcggctg
      961 cgttcacagg ctgaatcgct gtggctctcg atgccaaacc gggaatcaga tgaatggctc
     1021 acggtcagtc tcggtgtcgt tacttccttc cccttgaaat cttcgcttga ttccttgctc
     1081 agtgccgtga atctggcact tgaactggcc aaagatgctg gtggaaatcg ggtgatgatt
     1141 tcgccctccc cgctggacta atctcgttta aaaaaagcgg cgtcaagccg ccgcactcca
     1201 aacaggtatg cgactttttt ggttgaaaga cgtgtctcta cggcttgcaa cgatgccgcg
     1261 cccacggggc ggagaatggc ttgaatccga gattgaaacc ctcaaacagg agggtgtcaa
     1321 tatcctggtg tccctgttaa ccggatctga aatga
//
LOCUS       DAOQDO010002095         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_118888_length_1354_cov_2.692071, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002095 DAOQDO010000000
VERSION     DAOQDO010002095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118888_length_1354_cov_2.692071"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKZ53_25210"
     CDS             complement(<1..466)
                     /locus_tag="PKZ53_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ43805.1"
                     /translation="MTALLRKATIDDLLQVPNHGKAELVHGEIVSMPPTGGDPGRTAG
                     DIYASLRQYERQSGNGFAYPDNVGFRVNLPHRASFSPDAAFHIGLRTGMKYLEGAPVF
                     AVEVRSENDYGPEAELQMAEKRRDYFAAGTLVVWDVDLLSESVVKVYRSTDPE"
     gene            complement(637..>1354)
                     /locus_tag="PKZ53_25215"
     CDS             complement(637..>1354)
                     /locus_tag="PKZ53_25215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ43806.1"
                     /translation="SAQDSSPLIPEGLLTNRTYVIGLIVALLYATTQSSFYFTFVIFI
                     QVGLKFKPLWVALTLTPMALGFISGSIFASQLTKRFGQKTLTIGIILMGLGPLIAYLT
                     LLTNSEKINFIALSIGLMIQGAGMGLITTPLYNMILAKVRRAIAGAATGILTTVQQIG
                     GALGIAIVGVIFFGILGSRQALPNVTQAQAYTAAFMGSLWYYLGVCVVIFVLLQFLPR
                     QSTGNTGPLKSPVGKLPGLD"
BASE COUNT          363 a          324 c          359 g          308 t
ORIGIN      
        1 actcaggatc agtagaccga taaaccttga cgactgattc acttaacaga tcaacatccc
       61 agaccacgag tgtgcctgcc gcaaagtagt cgcgccgttt ttccgccatt tggagttcag
      121 cttccgggcc gtaatcattt tcacttcgca cttcaaccgc aaaaaccgga gctccttcaa
      181 ggtatttcat tccagtccgc aagccgatgt gaaacgcggc atccggacta aacgaagcgc
      241 ggtgagggag attgacccga aagccgacgt tgtccgggta agcaaagccg ttgcctgatt
      301 ggcgttcata ctggcgcaaa ctggcataaa tatcgccagc cgttcggcct ggatctccac
      361 ctgttggggg catagacaca atttctccgt gaacgagttc agctttcccg tggttgggaa
      421 cttgaagcaa gtcatcaatg gtggctttgc gaaggagagc agtcattcgg gtttcctcca
      481 gatgaattga ggttgaggaa acccgattgt accagcgaga ggagggaata ccaatttgga
      541 atcacttagt tcgattcgta gaaggttaag ttatttaatc aaataaactt aacgaacgac
      601 tgacgactga ctactgactc caagctgata tcaattctaa tcaaggccgg ggagttttcc
      661 gactggagac ttcaaggggc cggtattccc ggtactttgg cgagggagaa actggagcag
      721 cacaaagatc acgacacaca cgcccaggta ataccagagt gaacccatga aggcagccgt
      781 gtacgcttgc gcctgggtca catttggcag ggcctgacgg ctacccagga ttccgaaaaa
      841 gatcacgcca acaatggcaa ttcccagtgc tccaccgatt tgttgcaccg tggtcaaaat
      901 gccggttgcc gctccagcaa tggcccggcg gactttggcg agaatcatat tgtagagtgg
      961 tgtggtgatg agtcccattc ctgccccctg gatcataaga ccaatcgaaa gggcaataaa
     1021 attgatcttt tcactgttgg tcaggagggt gaggtaggca atcagtgggc ccaatcccat
     1081 caggataatc ccaatggtca gagtcttttg accaaaccgt ttggtgagtt gagaggcaaa
     1141 aatcgaccct gaaataaatc cgagtgccat tggggtgagg gtgagtgcga cccagagcgg
     1201 tttaaattta agtccaacct gaatgaaaat cacaaacgtg aagtaaaacg agctttgggt
     1261 tgtcgcgtaa aggagtgcca caatcaatcc gatgacatag gttcgatttg tgagaaggcc
     1321 ttccgggatc agcggggaac tatcttgcgc actg
//
LOCUS       DAOQDO010002096         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119041_length_1353_cov_2.869029, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002096 DAOQDO010000000
VERSION     DAOQDO010002096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119041_length_1353_cov_2.869029"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1101)
                     /locus_tag="PKZ53_25220"
     CDS             complement(241..1101)
                     /locus_tag="PKZ53_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ43807.1"
                     /translation="MASLGKRLPRNVEGEFFVDSTCINCDTCRQLAPDVFEDDGDYSF
                     VQAQPDNEKTRREATRALLACPTGSIGTTGENLSHQVISDFPFLIEDGVYYCGFNSAK
                     SYGGHSYFVQHPDGNWLIDSPRFLPHLTRPIEELGGIRFIFLTHRDDVAEAASYARKF
                     KAERIIHRDELSAQPDAERIIDGVETINFHPDFQIIPTPGHTRGHMVLCYRNRFLFTG
                     DHLWWSRVRQGLSASADYCWYSFPEQLKSLAKLKNYSFEWVLPGHGQRVHLPAEQMQH
                     ELGQFLKNRG"
     gene            1107..>1353
                     /locus_tag="PKZ53_25225"
     CDS             1107..>1353
                     /locus_tag="PKZ53_25225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43808.1"
                     /translation="MPLSVQTGVCFKNDEEYPIRFLLVTPFLHLGEQDQSVGGAVLQK
                     GQKGLKGLKVKKKQVCQMGRISSFFILNSSFFILNSSF"
BASE COUNT          357 a          309 c          369 g          318 t
ORIGIN      
        1 aaatcttgca tatcaaggag aagtgaggac atagaggaaa gagggctaag ggctaagggc
       61 ttggggctga agaaaacggg ctgaagactc gccagctcgg ggcggaagaa attgggtgga
      121 agaaattggg aattttgcct tagaaattgg gtggaagaaa ttgggaattt tgccttgaac
      181 ccgaagtctt cagccccaag cccgaagtct tcagccccaa gccctgagcc cgaagatttt
      241 tcagccccgg tttttcaaaa attggcctag ttcgtgctgc atctgctctg cgggaagatg
      301 cacccgctgg ccgtgtccgg gtaaaaccca ttcaaatgaa tagtttttga gttttgcgag
      361 tgatttgagt tgttcgggaa acgaatacca gcagtaatca gccgaagctg ataatccctg
      421 gcgaacacgg ctccaccaca gatggtcgcc cgtgaataaa aaccggttgc ggtagcacag
      481 gaccatatgt ccacgagtgt gaccgggagt cggaataatc tgaaaatccg gatgaaagtt
      541 gatggtttcg acaccgtcaa tgatgcgttc ggcatctggc tgggcggaaa gctcatcgcg
      601 atggatgatg cgttcggctt tgaatttgcg ggcatagctg gcggcttcgg ccacatcgtc
      661 acgatgcgtc aggaaaataa agcggattcc gccaagctcc tcaatgggac gagtcaggtg
      721 cggcaagaag cggggcgagt caattaacca gttcccatcg ggatgttgca caaaatagct
      781 atgtcctcca taggactttg ccgagttaaa cccgcagtag tacacaccat cttcaatcaa
      841 aaatgggaag tctgaaatca cctggtgcga caggttttcc ccagttgttc caatggatcc
      901 ggttgggcag gcaagcaggg cacgcgtggc ttcgcggcgg gttttttcat tgtcgggctg
      961 tgcctggacg aaggaatagt caccgtcatc ttcaaacacg tcaggggcaa gctgacgaca
     1021 ggtatcacag ttgatacagg ttgaatcaac aaaaaattcg ccttcgacat tgcggggaag
     1081 ccgctttcca agacttgcca ttgtgattgc ccctttcagt ccaaaccgga gtgtgcttca
     1141 aaaatgatga agagtaccca atccggttcc tgcttgtcac cccattcctc cacctggggg
     1201 aacaagacca gtcggtcgga ggggcagtgt tgcagaaagg acagaaagga ctaaaaggat
     1261 taaaagttaa aaagaagcag gtgtgtcaaa tgggtcggat ttcttcattc ttcattctca
     1321 attcttcatt cttcattctc aattcttcat tct
//
LOCUS       DAOQDO010002097         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119227_length_1352_cov_2.834233, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002097 DAOQDO010000000
VERSION     DAOQDO010002097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119227_length_1352_cov_2.834233"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1352)
                     /locus_tag="PKZ53_25230"
     CDS             complement(<1..>1352)
                     /locus_tag="PKZ53_25230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43809.1"
                     /translation="YGVWLSNGQAVIKLAQALQAQAQDPVFGHHPLRHLTRELLVQSD
                     PVPQLQAALDHITALVHQIQATTSQLPVQLASASTIDELTALCSYAQQLMPLVELDCL
                     DLLNRKSKGYDELRDVFHQFLECSEALAQAQSRTTHWRTRLPLSEITLMLPATRQVES
                     SFLGVLTPTYWKLRKRLKAAYDFQAHPIKPTWSTILEQLQREYELFEALSGLRKAIHT
                     RFRVEDPRPLRELIERLHAAELALYPTQKAFRAGLISGKIDPAVCVQLAQIRHPVELL
                     TIELEELLDPFANRTLADISADLTQLRANLSVLGTLQPELAELAGGPVELHRAVCDFP
                     GTPRQLEMALLLKIIARARRTERAVFQADATKLANQADDARRLHFKLMASTAQMVSTR
                     PHETFRQKLNKAELPAARLQDAEKSWKKGFNRGRRELEREFSKVMRFRSIRDLAEGET
                     G"
BASE COUNT          293 a          341 c          397 g          321 t
ORIGIN      
        1 ccggtttcac cctcggccag gtcgcggatt gaccgaaatc gcatcacctt gctgaattct
       61 cgttcgagtt cacgtcggcc ccggttaaat ccttttttcc agcttttttc agcgtcctgc
      121 aacctggctg cgggtaattc cgctttgttg agtttttggc gaaaggtttc gtgcggtcgg
      181 gtgctgacca tctgagccgt gctggccatc aatttgaaat gcaagcggcg agcgtcatcg
      241 gcctggttgg ccagtttggt ggcatccgcc tgaaaaacag cccgctctgt ccgtcgggca
      301 cgagcgatga ttttcagcag caacgccatt tccaactggc ggggcgtccc gggaaaatcg
      361 catactgctc gatggagttc caccggacct ccggccagtt cggccagttc gggctgtaat
      421 gttcccaaca cagacaggtt ggcccgcaat tgagtcagat ccgccgaaat atcagccagg
      481 gtgcgattgg caaacggatc cagaagttct tcaagttcaa tggtgaggag ttcgaccggg
      541 tggcggattt gagcaagctg aacacagaca gcaggatcaa tttttcctga aatcagcccg
      601 gcccgaaatg ctttttgggt cggatagagc gccagttcgg cggcatgaag acgctcgatc
      661 agctcgcgga gtggtcgagg atcttcgact cgaaaccggg tgtgaatcgc tttgcgcagg
      721 ccagacagtg cctcaaacaa ctcatattcg cgctggagtt gttcgaggat ggtgctccag
      781 gttggtttga ttggatgcgc ctgaaaatca taagccgctt tcaatcgttt gcgcagcttc
      841 cagtaggtgg gtgtcaacac gcccaaaaat gaagactcaa cctgcctggt tgcgggtaac
      901 atcagggtaa tttctgagag gggcagtctg gtccgccagt gagtcgtccg actttgggcc
      961 tgggcgagtg cttcggaaca ttcaagaaat tggtgaaaca catcccggag ttcgtcatag
     1021 cccttcgatt tccggttaag caggtccaga cagtcaagtt ccaccagcgg catcaattgt
     1081 tgggcatatg agcaaagggc ggtcaactcg tcaatggttg aagctgaagc aagttggact
     1141 ggtaactggc tggttgtggc ctgaatttga tgaaccagag ctgtgatgtg atcaagcgcg
     1201 gcttgaagtt ggggcaccgg atctgattga accagcaact ctcgggtcag gtgtctgagc
     1261 ggatgatggc caaaaaccgg atcctgagcc tgcgcctgaa gtgcctgggc aagtttgatg
     1321 accgcctgtc catttgagag ccagacccca ta
//
LOCUS       DAOQDO010002098         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119296_length_1352_cov_2.125675, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002098 DAOQDO010000000
VERSION     DAOQDO010002098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119296_length_1352_cov_2.125675"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKZ53_25235"
     CDS             <1..431
                     /locus_tag="PKZ53_25235"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNJ43810.1"
                     /translation="LRVARSHHKSRTGQWATQWGGWVPGVKVWFCPVEFWAMTALQMQ
                     RQGHQLEAIFDESVFYARDFVVEEDNFQQPGLRDDRTADWDLFSLSPGASAGDLKRAY
                     RDLVDVWHPDRFAHNPRLQAKAETMTKLINAAYQRLQVAA"
     gene            complement(518..>1352)
                     /locus_tag="PKZ53_25240"
     CDS             complement(518..>1352)
                     /locus_tag="PKZ53_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43811.1"
                     /translation="AMVTQDPEPAEPAEVESCDEPTPYNKEEETFLSEIKERVLVGIQ
                     AESDLSPDETDDWEPPVQAEESVFWVDGLTTQTENEITDSGELNPDEFSGNAVNSDSL
                     TTLENELELPPGTFQNELKFICEEPKETRRAFARERYQKLRDHLEKCAPNFGFSELRW
                     KFRSIFGGAICDLLEKVDVPLRFTDKCYACQHRHAQLKRVRKCFMDDTQDDMKEAREF
                     GEYLRECLNKGLTLDDAFEPLERLIAPDDDEVRNVVGFIKRGLKQCLRNEEESGENQA
                     Q"
BASE COUNT          326 a          355 c          301 g          370 t
ORIGIN      
        1 cgttaagggt tgcgcgcagc catcacaaat ctcgaactgg tcaatgggcc acacaatggg
       61 gaggctgggt accaggggta aaagtttggt tctgcccggt tgaattctgg gccatgaccg
      121 cgctccaaat gcagcgccag gggcatcagt tggaagcaat atttgacgag tccgtttttt
      181 acgctcgcga ttttgtcgtt gaggaagaca attttcagca accaggtttg cgcgatgacc
      241 gaacagcaga ctgggatctc ttcagtttgt cccctggggc aagtgccggt gatctcaagc
      301 gagcctaccg ggatctggtt gatgtctggc atccagatcg cttcgcacat aatccccggt
      361 tgcaagcaaa ggccgaaacg atgacaaaac tcatcaatgc ggcgtaccaa agacttcaag
      421 tagcagctta aatcaaagtt taaatcaaag taatccaggt ctgataactg aaaaatctca
      481 attcatgtag ttttgtccgt cattatcgga ctcctgatca ttgtgcctgg ttttcgcctg
      541 actcttcctc attgcgcaaa cactgcttaa gccctcgttt tatgaaaccc acgacatttc
      601 gtacctcatc gtcgtcagga gcaatcaatc tctccagtgg ctcaaacgca tcatcaagcg
      661 ttaacccctt gttgagacac tctcttaaat actcgccgaa ctcccgtgct tctttcatgt
      721 catcctgagt atcatccata aaacacttcc tcactctttt caattgagca tgacgatgct
      781 gacaagcgta acatttgtca gtgaatcgca gcggtacatc aactttttca agcaaatcac
      841 aaatcgcccc accaaaaata gacctgaatt tccatctcaa ttcggaaaat ccgaagttcg
      901 gggcgcactt ctccaggtga tcccgtagtt tttggtaacg ctcgcgggca aatgcgcgtc
      961 gggtctcttt cggttcctca caaatgaatt ttaactcatt ctggaacgtg cctggcggca
     1021 actccagttc attttcgagg gtggtcaggg agtcggaatt tacggcattt cccgaaaact
     1081 catcaggatt aagttctcca gaatcagtta tttcgttttc ggtttgggtg gtcaatccat
     1141 ccacccaaaa taccgattct tcagcctgaa ctggtggctc ccaatcgtcg gtttcgtcgg
     1201 gggacaaatc cgattcggct tgaattccca ccagcaccct ttcttttatt tcagataaga
     1261 aagtttcctc ctccttatta tagggggtcg gttcgtcgca gctttcgact tcagccggtt
     1321 cagccggttc cggatcctgg gtcaccattg cc
//
LOCUS       DAOQDO010002099         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119430_length_1351_cov_2.375000, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002099 DAOQDO010000000
VERSION     DAOQDO010002099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119430_length_1351_cov_2.375000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKZ53_25245"
     CDS             complement(<1..254)
                     /locus_tag="PKZ53_25245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43812.1"
                     /translation="MAKSKNTPLKESPWIEVGNGYALALKSQKLICRNAAGELLTSLP
                     KIVRESEVADQLRSTLEFLELHKQACLRTVETWMVRSLPIP"
     gene            complement(558..>1351)
                     /locus_tag="PKZ53_25250"
     CDS             complement(558..>1351)
                     /locus_tag="PKZ53_25250"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNJ43813.1"
                     /translation="LGAGNWRTRTEDRLSPPILKAGARLQIEGSSLQAHILETSVVSP
                     RSVTLEFNLDESATWREMYRAGHPIQYSHLNGDLDLWDVQTAYASRPWAMVMPSAGKP
                     LQWKLLLDLMRHGVNLAALTHAAGISASGDPVLDALLPLPERYDLPLATIEAIDSTHN
                     QGGRVIAIGTSVTRALEGNYARHGKLVAGLGMTDLILQPGFERQIVDGILSGMHEATE
                     THFRLLSCFLDESLLQAAVEHAEASGYLSHEFGDSCLILPETQII"
BASE COUNT          336 a          355 c          333 g          327 t
ORIGIN      
        1 gggatgggca atgaccgcac catccacgtt tcaacggtcc gcaggcacgc ctgtttatgg
       61 agttccagaa actcaagggt tgatcgaagc tggtcagcta cttcactttc gcgaacaatt
      121 ttgggaagcg acgttaacaa ctcgcccgcc gcattgcgac agatgagttt ctggcttttg
      181 agtgccaggg cataaccatt tccaacctca atccaggggg actctttcag tggggtattt
      241 tttgatttgg ccattccggg aattcttcca ttcttcagga tttactggcg gaattttgca
      301 tgacaaattc cagtttgata ccaggatttt tcccgagcat atcggaacca aagagccaaa
      361 aacaaccact tcgttatgta cacacgacaa aatacataag gaacatcagg gacagacaga
      421 ctgatgttcc tctgtatttt tatgtgatga gggttcagct actgatgccg tttggcaatg
      481 aaaccctcct gttacggaga tgctaaatta ctgaagaaat tgtatttcca tgaaccctga
      541 accctgaatc ccgaacccta aattatctga gtttcaggca gaatgaggca ggaatctcca
      601 aactcatgac tgaggtagcc gctggcttcc gcatgctcaa ccgcagcctg caagagtgat
      661 tcgtccagaa agcagcttaa caaccggaaa tgggtttcgg ttgcttcgtg cattccagag
      721 agaatgccat ccacgatctg acgctcaaat cctggttgca aaattaaatc ggtcattcct
      781 aatcccgcaa ccagctttcc atgcctggca taattccctt caagcgcccg agtcactgac
      841 gtcccaatgg caatcacccg cccaccctgg ttgtgggttg aatcaatggc ttcaatcgtg
      901 gccagtggaa ggtcataacg ttctggcaat ggaagcaagg catcgagcac cggatcaccg
      961 ctggcggaaa tcccagcggc atgggtgagc gccgccaggt ttaccccgtg gcgcatcaga
     1021 tcaagcaaga gcttccattg caagggtttc cccgctgatg gcatcaccat cgcccaggga
     1081 cgcgaggcat aggcggtctg cacatcccac aagtccaggt cgccattgag atgagaatac
     1141 tggattggat gccctgcccg gtacatttcg cgccacgtcg cggattcatc cagattgaat
     1201 tcaagtgtca cactccgagg tgatacaacg gaagtttcaa gaatgtgtgc ctggagcgat
     1261 gaaccttcaa tctgtaatcg tgctccggct tttaaaatcg gcgggctgag ccggtcttcg
     1321 gtgcgtgttc gccagtttcc tgcgcccagg a
//
LOCUS       DAOQDO010002100         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119566_length_1350_cov_3.111197, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002100 DAOQDO010000000
VERSION     DAOQDO010002100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119566_length_1350_cov_3.111197"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKZ53_25255"
     CDS             <1..684
                     /locus_tag="PKZ53_25255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ43814.1"
                     /translation="LYDPTGIRQHWCARTWCKLIAWTVGMKVIIHGRENLDLSRPAVY
                     MSNHQSYMDIPTLFAFLPFQFRIFAKKVLFYIPFMGWHLWRAGNIPVDRGNHSATTKV
                     LNLGVEKLRSGVPIVVFPEGTRGTELRRVKRFKAGAFKIAQMAKVPVVPITIVGTAHI
                     LQKDSLLFRPGTVHLFIDPPIETADTDDQRLSDVVAQVQQIMNSNLERVYGQSSAGTA
                     GSVPVSILR"
     gene            629..>1350
                     /locus_tag="PKZ53_25260"
     CDS             629..>1350
                     /locus_tag="PKZ53_25260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNJ43815.1"
                     /translation="MVNLQPVPPDPYPFPYSAEGSWPLPIDPDKPAWGPGSGLLMWGI
                     SILAIVIMQLPAAMLWAGEIYFRTGKMPVLSPTVQDPRLVLYSVLSTIVAHLLTFAAC
                     WMLVRTSAPNVKFWNALGWGWTKDFRWYHCWGIILSFLALGVAVSQLIPHKETDFDRI
                     LKIGPSIRIAVSIVALATAPLVEEVVYRGMLYSGFRKMVEPWAAITLVSFLFLLVHVP
                     QYWGGWDGLTVLALLSITLTIVR"
BASE COUNT          268 a          351 c          370 g          361 t
ORIGIN      
        1 ctctatgacc caaccgggat tcgccagcac tggtgtgccc gaacctggtg caaattgatt
       61 gcctggacag tcggaatgaa ggtgatcatc cacgggcggg aaaacctgga cttatcccgt
      121 ccagccgtct atatgtccaa tcaccagagt tacatggaca ttccgacgct atttgccttt
      181 ctgccgtttc agttccggat ttttgccaag aaggtgctct tttacattcc ctttatgggc
      241 tggcatctgt ggcgtgccgg gaatatcccg gttgatcgtg gcaatcactc cgccacgacg
      301 aaagtattga atctgggggt cgaaaagctt cgctcaggtg tgccgattgt ggtgtttccc
      361 gaagggacgc gtggtactga actgcgacgg gtcaaacggt ttaaagctgg cgccttcaaa
      421 atcgcgcaaa tggccaaagt tccggtggtg ccaattacta tcgttggcac cgcccacatt
      481 ttgcagaaag attcactgct gtttcgtcct ggaaccgttc atctttttat tgacccgccg
      541 attgaaacgg ctgacaccga tgaccaacgc ctttcggatg tggtagccca ggttcagcaa
      601 atcatgaatt caaatttgga gcgcgtttat ggtcaatctt cagccggtac cgccggatcc
      661 gtacccgttt ccatactccg ctgaggggag ctggcccttg ccgattgatc ctgacaagcc
      721 agcctggggt cctggttcgg gcttactcat gtggggaatc agcattttgg cgattgtgat
      781 tatgcaattg ccggcagcta tgttgtgggc aggtgaaatc tattttcgaa ccggaaaaat
      841 gccggtactt tcgcccacgg tacaggaccc gcgactcgtc ctgtatagcg tgttatcaac
      901 cattgtggcg cacctgctta cgtttgccgc gtgctggatg ctggtgcgaa ccagcgctcc
      961 gaatgtcaaa ttctggaatg cgctgggatg gggctggacc aaagatttcc ggtggtatca
     1021 ctgctgggga attatcctga gctttctggc gcttggcgta gccgtttcgc aattgattcc
     1081 acacaaggaa accgactttg accgcattct caagattggg cccagcatcc ggatcgctgt
     1141 ctcaattgtg gcgctggcca ccgcgccgct ggttgaagaa gtggtctatc gtgggatgct
     1201 ctatagcgga tttcgcaaaa tggtcgaacc ctgggcggcc attacgctgg tttcttttct
     1261 ctttttgctt gtccacgtcc cccagtactg gggcggttgg gatggtttga cagttctggc
     1321 cctgctcagc atcactttga ccattgtgcg
//
LOCUS       DAOQDO010002101         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119604_length_1350_cov_2.538224, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002101 DAOQDO010000000
VERSION     DAOQDO010002101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119604_length_1350_cov_2.538224"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKZ53_25265"
     CDS             complement(<1..810)
                     /locus_tag="PKZ53_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43816.1"
                     /translation="MNIVLPKESRGYVYYRIFTVEMNNFDVERLLPALFYLIVTKGRQ
                     RAKIPNDPTAFDVYIERLSKHPAIAGFNTASGRRLLDRWIRSSVIQVGKKGRAHKDEQ
                     IECIQPLTFLAYKSGLPKESSRHRRVHLFLYWLMCSVLTRSPHSLSEIQAKDALDKVF
                     REAFGSGIVIGGGPKFDGKYNGFTNLDLQTLLCLYYLDGFAPTEISNLREANSPFGPA
                     LPWQAENFGKDLLQYVMQYATKVPILALTRSLLALINFELFTYTVKMVYAVN"
     gene            complement(807..>1350)
                     /locus_tag="PKZ53_25270"
     CDS             complement(807..>1350)
                     /locus_tag="PKZ53_25270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43817.1"
                     /translation="GRALPQPDRLLPYRYFDDFLAALSNQQHDHKLLSQLAGGMSRAD
                     GVPAKASEGFLAFRMNETETLDFTVVKQFPLNEFEVRRSATQSRYVEAISDQLLFRYQ
                     NGYHQLSIGLDLFEFLQRAADGLVAGAEEQKAMSEDLTLFKNMLLANPTRQVTLIEAG
                     RSIHQVSIQDGKIVREVQSK"
BASE COUNT          370 a          318 c          311 g          351 t
ORIGIN      
        1 attcacggca tataccattt tgacggtata agtaaacaat tcaaaattga tcaaagccag
       61 gagcgaacgt gtcagggcaa ggattggaac ttttgttgca tattgcatga catattggag
      121 aagatccttc ccaaaatttt cagcctgcca gggtaaggcg ggaccaaatg gggagttggc
      181 ttctctcaag tttgaaattt cggttggtgc aaatccgtcc aaatagtaca aacaaagcag
      241 tgtttgcaga tccagattcg taaagccatt atatttccca tcaaatttag gaccgccccc
      301 aattacaatc ccagacccaa acgcttcacg aaagacctta tccagggcgt ctttagcctg
      361 gatttcactc aaagaatgcg gagaccgggt taaaacagaa cacataagcc agtaaagaaa
      421 taaatgaacc cgccggtggc gtgaactttc ttttggaagc cctgacttgt atgccagaaa
      481 tgtcaggggc tgaatacatt cgatttgctc atccttatga gcgcgtccct ttttcccaac
      541 ttgaatcacg ctggatcgga tccaccgatc cagaagccgc cgaccagagg cggtgttaaa
      601 tccagcaatg gctgggtgct ttgaaagacg ttcgatatag acatcaaatg ctgttggatc
      661 attcggaatt ttggctcgct gtcttccttt ggtcacaatc aaataaaaca gtgctggtaa
      721 cagacgttcg acatcaaagt tattcatctc tacagtaaag attcggtaat atacataccc
      781 acggctttct ttgggcaaca cgatgttcat ttggattgta cctcccgcac tattttaccg
      841 tcctggattg atacctgatg gattgagcgt ccagcttcaa tcaaagtgac ctgccgggtt
      901 ggattagcca gcagcatgtt tttaaacaag gtcaggtctt cgctcatcgc tttttgttct
      961 tcagcaccgg ccaccaatcc gtcagccgca cgctgcaaaa attcaaacag atcgagtccg
     1021 atactgagtt ggtgatatcc gttctgatac cgaaacagaa gctggtctga aatggcttca
     1081 acgtatcgcg attgggttgc tgatcggcgc acttcaaatt cattaagagg gaattgcttc
     1141 accacggtaa aatcaagggt ttcagtttca ttcatgcgga acgccaggaa tccttcgctg
     1201 gctttggctg gtaccccatc agcccgtgac atcccacctg ccagttgaga caggagtttg
     1261 tgatcgtgtt gctgattgga aagagccgct aaaaaatcat caaaataccg gtatggaagc
     1321 agccggtcag gctgggggag cgcacgacct
//
LOCUS       DAOQDO010002102         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119657_length_1350_cov_2.028571, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002102 DAOQDO010000000
VERSION     DAOQDO010002102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119657_length_1350_cov_2.028571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKZ53_25275"
     CDS             <1..202
                     /locus_tag="PKZ53_25275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43818.1"
                     /translation="AGFASLTVQPMPNNACCTMPGLVWYAPLVPLQNRKVGYTVNASY
                     EGSQVGMPWSRASENSAFYGTF"
     gene            complement(899..>1350)
                     /locus_tag="PKZ53_25280"
     CDS             complement(899..>1350)
                     /locus_tag="PKZ53_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43819.1"
                     /translation="ALRQAMDKGVGIGQLGGEGWGWSGVSYTDVQLKVFNSKLNPAEL
                     QTLCSTKALSVARSLSSNATFQTQTVNTWQKSVIQFDLWFQDYGGTATVEFWIQSAKT
                     GSLIFVFMHANGPECEEVSEILSSMGIIQPVAGLQNSTSNLEDLRKH"
BASE COUNT          358 a          311 c          325 g          356 t
ORIGIN      
        1 ggctgggttt gcctcattaa ccgtgcaacc gatgccaaat aatgcctgtt gtaccatgcc
       61 ggggctggtg tggtatgcgc cgttagtgcc gctccagaat cgaaaagtcg gctataccgt
      121 caatgccagt tatgaaggca gtcaggtcgg catgccctgg tcacgtgcct ctgaaaacag
      181 tgctttttac gggacattct gactggcagg ggaacgacgt aaaacacaga gttttaagct
      241 acttacttgc cgagcgattg cctgaatgaa gagcacgccc aagcgtgaac ccggcacaga
      301 aatcagtttc agaaatactt tcaatcaaat cgtgaaacca tccttctgtt cacaaaaaac
      361 gaaaggatgg ttttctattt ttgggtgttg gctggctgct gagagagttc agtgtcatcc
      421 tggagtccac acccaatgac cgctgcgcat tttccatggc tgtttacctg gagtccatcc
      481 atccaggtgt cgaaagccga ggtgggctct gcacaagaac cggaactcaa ttctgagtct
      541 tctgagtcca tacctggttc atcttcaacc aaaccggtaa ccgcttgaaa tcgaaatgat
      601 tcctgttcca gccagtgggc cttttctctt attttttttc gtgattctaa aagcattgaa
      661 actcctgaaa aaatggaagg tgttcaaaaa aaccggtgtc aactcagagt cagttcctgg
      721 gtggtaccct gaggtatctg aagtgtgacg ggttttgaag tctgagtctt acgtaaaact
      781 tgagttcagg ttcaaccgag aaatcaaagt catttgaaga aatgctctgc cactcggata
      841 caaactaagt tgtgataact gtaggcctga catattgagc tgaagatcaa acacgggatc
      901 aatgctttct cagatcttcc agatttgaag tcgagttttg aagccccgcg acaggttgga
      961 taatgcccat tgaactgaga atttcagaaa cttcttcaca ctcagggcca tttgcatgca
     1021 tgaaaacaaa gatgagtgag ccggttttgg ctgactgaat ccaaaactca actgtggctg
     1081 ttcccccata atcttgaaac cagaggtcaa attgaattac ggatttctgc caggtattga
     1141 cagtttgagt ttgaaaggtg gcattgctgg aaagggacct ggcaacggat aaagcttttg
     1201 tcgaacacag ggtttggagt tcagcgggat ttagtttgga attgaaaact ttgagctgaa
     1261 catccgtgta gctaaccccg ctccaccccc aaccttcgcc gccaagttgg ccaatgccga
     1321 cacctttatc catcgcttgc cgcagggcta
//
LOCUS       DAOQDO010002103         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_119966_length_1348_cov_2.232019, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002103 DAOQDO010000000
VERSION     DAOQDO010002103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119966_length_1348_cov_2.232019"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKZ53_25285"
     CDS             complement(<1..676)
                     /locus_tag="PKZ53_25285"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43820.1"
                     /translation="MNSAILNPTPWYRLFCVLLIVCSIDTLCARPIFQSPTQDETRLA
                     AQQMCTEGDQLRKTPTAETLTQAIQKYQVALEYWQKLGDQASQAETHAKIGNSYLELG
                     NYSKALAAFEQALHFAPAAKNPKWESDLLIRAGSIQSRLNQFSQAQDKLSRGLAIKHE
                     LGDATGEANALTELGVIASETGDHQKAIQSFSDALARFRTLGNQRGELFIQFYLGQLY
                     INRNEHQ"
     gene            949..>1348
                     /locus_tag="PKZ53_25290"
     CDS             949..>1348
                     /locus_tag="PKZ53_25290"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43821.1"
                     /translation="MNCSSITFRLSKWLLIVAIPWGTVFAQAPQSDTFVSLLQQAQAK
                     TNAHQWAQAAPLWEKVVSLNPVHGRFWELLAEARFQNKQYPEAIQAYEKALELRIGFP
                     ADQAYNIARCHAARGDKEASLTWLERAFDLG"
BASE COUNT          308 a          339 c          370 g          331 t
ORIGIN      
        1 tctggtgctc gttgcggttg atgtacaact gacccaggta aaactggata aagagttctc
       61 cgcgttgatt cccaagcgtg cgaaaccgtg ccagcgcgtc tgaaaacgat tgtatggctt
      121 tttgatgatc gccagtttct gaggcaatga cgcccagttc ggtcagggca ttggcttcac
      181 ccgtggcgtc gcccagttcg tgttttatgg ccagtccgcg actcagtttg tcctgggctt
      241 gagaaaactg attgagccgc gactgaattg acccagctcg aatcagcagg tccgattccc
      301 actttggatt tttggccgcc ggggcaaagt gcagggcctg ttcaaacgca gcgagggctt
      361 ttgagtagtt gcccaattcc aggtaactat tgccgatttt ggcgtgagtc tcggcctgac
      421 tggcctgatc tcccagtttt tgccagtact ccagggcgac ctggtatttt tgaatggcct
      481 gggtgagtgt ttccgccgtt ggggttttcc gaagctgatc accttcggta cacatctgct
      541 gggcagcaag gcgggtttca tcttgcgttg gcgattgaaa aatgggacgg gcgcacagcg
      601 tgtcaatcga acacacaatg agcaacacac aaaagagtcg gtaccagggc gtaggattca
      661 ggattgcgga gttcatggga aaggcttagg ggtcaagcgg ttgcgaaagc ccagtttttt
      721 caactgcttc catttctttg ccggaaaaga tgagttttag atggagggca ggcccagaaa
      781 gaatagcaga agacgcagcg tattgaaatt tagagcgagc caacccgtca gcgattttat
      841 ctgcggagaa tcactttttt ctttctgtgt tgttaacctc tgaccctgca ccaccgtcat
      901 agcggtacac aaaaccggaa accggtgctt tctggcaggg aggtccaaat gaattgttcc
      961 agcattacat tcaggttgag caagtggctg ctgattgtgg cgataccgtg gggaaccgtt
     1021 tttgcccagg cgccacaatc agacacattt gtcagcctgc tccaacaggc tcaggccaaa
     1081 acgaacgcac accaatgggc tcaagccgct ccgttgtggg aaaaagtcgt gtccctcaac
     1141 ccggttcacg gacgattttg ggagttgctg gccgaagcac gctttcagaa caagcaatat
     1201 cccgaagcca ttcaggcata tgaaaaagcg cttgaactcc gcatcggttt tcctgccgat
     1261 caagcctaca acattgcccg ctgccatgcc gcccgtggtg acaaagaggc atccttaaca
     1321 tggcttgaac gagcctttga cctcgggt
//
LOCUS       DAOQDO010002104         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120151_length_1347_cov_2.393189, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002104 DAOQDO010000000
VERSION     DAOQDO010002104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120151_length_1347_cov_2.393189"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKZ53_25295"
     CDS             <1..483
                     /locus_tag="PKZ53_25295"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNJ43822.1"
                     /translation="TEASPPPPPATPSQPAQPNPEPVTFPLKESPPLFSETNSNQPAP
                     EPPGIGSLLGRTVLVLGSVIAVLVSLAWGLQRYGVNRTAQFGAQDMLTVLKVVPLGER
                     RFLSVVRFGNQILLLGSTAQSITVLASHTEPTALSDTESTFSGCSVAEMLDSVNFKEP
                     "
     gene            483..1232
                     /gene="fliP"
                     /locus_tag="PKZ53_25300"
     CDS             483..1232
                     /gene="fliP"
                     /locus_tag="PKZ53_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722487.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNJ43823.1"
                     /translation="MAYLSPILTQLAFSPWLAALVAAPASGSPPTLPIDPAKDPTGTT
                     LPLQIVITLTLLTFIPAILVSMTCFTRLIIVFHFLRQAIGTQEVPNNQILISLALFLT
                     LFVMAPVVSQVNQAAVQPMIEKKIGYQEALTRALVPIRGFLFKHTRETDLALFVRLSH
                     APRPQKADDVPTTTLIPAFMISELKTAFQIGFVLFIPFLVIDLAVSSVLLSLGMMQVP
                     PVLISTPLKLLLFVMVDGWYLIIGSLVKSLL"
BASE COUNT          269 a          362 c          327 g          389 t
ORIGIN      
        1 accgaagcgt ccccgccgcc gccgccagcc actccttccc agccagctca gccaaacccc
       61 gagccggtga catttccgtt aaaagagtca ccccctctct tttccgagac taactcaaac
      121 cagccagccc ccgaaccgcc ggggatcggc agcctgcttg ggcggacggt tctggtttta
      181 gggagcgtga ttgcggtgct ggtcagtttg gcatggggtt tgcagagata tggggtaaat
      241 cggacagctc aattcggcgc ccaggacatg ttgacagtct tgaaggttgt gcccctcgga
      301 gaacgccggt ttttgagtgt tgtgcgcttt ggtaatcaga tcctgttgtt gggatcaacg
      361 gctcaatcaa tcacggtgct ggcttctcat accgaaccca ccgccctttc agacactgag
      421 tccacatttt caggatgctc tgtggccgag atgctggatt cggtcaattt caaagaacca
      481 tgatggcata cctttccccc atactgaccc agcttgcctt ttcaccctgg ctcgccgctc
      541 tcgtggccgc cccagcttcc ggttcacctc ccacgcttcc gattgatcca gccaaagacc
      601 caactggaac gaccctcccg ctgcaaatcg tcattactct cacattatta acttttatcc
      661 cggccatcct ggtctcgatg acctgtttta cccggctgat catcgtgttt cattttttgc
      721 gacaggcaat tggcacccag gaggtgccaa ataaccagat tctcatcagt ttagcccttt
      781 ttttgacact gtttgtaatg gcaccggtgg tcagccaggt caatcaagcc gccgtacagc
      841 cgatgattga aaagaaaatc ggctatcagg aagccttgac ccgggcactg gttccgattc
      901 gggggttttt attcaagcat acccgcgaaa cggatctggc attgtttgtg aggctctcgc
      961 atgcgccccg cccccaaaaa gccgatgacg tgccgactac gacgctgatt cccgctttta
     1021 tgatttctga actcaaaacc gcctttcaaa ttggatttgt tctatttatt ccatttttgg
     1081 tgattgatct ggcggtttca tcggtcttgt tgtcgttggg gatgatgcag gtcccgccag
     1141 ttctgatttc aactccgtta aagctcttgt tgtttgtgat ggttgacggg tggtatttga
     1201 tcatcggttc gctggtgaaa agtttgttgt aagtaccttg tcataaattt tctgagatat
     1261 tgggtttggt aagtgcttga ttcttttccg tgttttccgt gttttccgtg gttaaaatgt
     1321 ttggaaattt tcggtaaggt acttacc
//
LOCUS       DAOQDO010002105         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120274_length_1346_cov_3.166538, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002105 DAOQDO010000000
VERSION     DAOQDO010002105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120274_length_1346_cov_3.166538"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PKZ53_25305"
     CDS             <1..1201
                     /locus_tag="PKZ53_25305"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HNJ43824.1"
                     /translation="RYSNTDNRNKWVIYLQGGGDCGSGQACAQRWCSIETNFGANKMS
                     SSFAPDKGIRGNGLLSPDSSLNNFATWNQVFVYYCSSDQWSGTAANVQLSATGVNGQS
                     FQYTIHFKGAEIFDAVVSMLRRENKAKVKYRDQAGKKLVLPDLDDATTVVFAGASAGS
                     GGVRNNTDRLQSLLRQNNTNCTGSQCPLQYFAVIDSGFPPDMSGLDFSQSVPCEKASL
                     CSYEAFIQYQWNVVQRGTWGARPDQSCLDFHQKDGTEFECANLVHVMQNHLTSPYFTR
                     QDTQDQLLLGNNVDAGLTTPQSFGDLVHAQLTNLVNLDSFAEEGSVRSGGAKLTTPGV
                     FGAQCTDHETLTNNQDFFEVKVLHNGQMYSAHDVLTNWLRGQQPSVVIRPFTTPGAAP
                     ECPPEAK"
BASE COUNT          341 a          343 c          339 g          323 t
ORIGIN      
        1 aagatattcc aacaccgata accgcaacaa atgggtcatt tatctccaag gtggcggtga
       61 ttgtggctcg gggcaggcct gtgcccagcg ctggtgcagc attgagacca attttggcgc
      121 caacaaaatg agttcttcgt ttgcacccga caagggcatt cgtgggaatg gacttctcag
      181 cccggattca tcactgaaca atttcgccac ctggaatcag gtatttgtct attattgcag
      241 ctcagatcaa tggtccggta cggcagcgaa tgttcagctc tcggcaactg gggtcaatgg
      301 gcagtctttt cagtacacga ttcattttaa aggggctgaa atctttgacg ctgtggtttc
      361 aatgctccgg cgggaaaaca aagccaaagt gaaatatcgc gatcaagccg ggaaaaaact
      421 cgtcctgcca gatctggatg atgccacgac ggttgtattt gccggtgctt cggcagggtc
      481 aggcggagtt cggaacaata ctgaccggtt gcaatcgctc ttgcggcaaa acaacaccaa
      541 ctgtacaggc tctcaatgtc cactccagta tttcgccgtg attgactccg ggtttccgcc
      601 tgatatgtct ggactggatt tctcacagtc tgttccgtgt gaaaaagcct cgttgtgtag
      661 ttatgaagca ttcatccagt atcaatggaa tgttgtgcaa cgtggaacct ggggcgctcg
      721 cccggaccag tcgtgccttg attttcacca aaaagacggg actgaatttg aatgcgccaa
      781 cctggttcac gtcatgcaaa atcatttgac ctcgccttac tttactcgac aggataccca
      841 ggaccagctt ttgctcggaa ataatgtgga cgctggctta accactcccc agtcctttgg
      901 tgatctggtt cacgcccaac tcaccaatct ggtcaatctt gattcgtttg ctgaagaagg
      961 aagtgttcga agcggagggg ccaaattaac cactcctgga gttttcggcg cccaatgcac
     1021 cgaccatgaa accctgacaa acaaccagga tttcttcgag gttaaggttt tgcacaatgg
     1081 ccagatgtat agcgcccacg atgtcctgac aaactggctg cgaggacagc agccttccgt
     1141 agtgattcgt ccctttacga ctcccggcgc ggcacctgaa tgcccgccag aggcaaaata
     1201 ataccatttt gggatcaggg tatcgggttc agggtttaaa agacgaaaag gacggaaagg
     1261 acggaaagga cgaaaaggac ataaattcga aaaccctgaa ccctgaaccc tgaaccctgt
     1321 catcttgcca ccgtatcatc cgggcc
//
LOCUS       DAOQDO010002106         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120287_length_1346_cov_2.829589, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002106 DAOQDO010000000
VERSION     DAOQDO010002106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120287_length_1346_cov_2.829589"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKZ53_25310"
     CDS             <1..437
                     /locus_tag="PKZ53_25310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43825.1"
                     /translation="HRVGRFLNFATEETLTPPFQDSWLHSDQDIRLDWSWDYIQGIAN
                     ISEGCGVFLRPFAILYSGPPHPDEMQVKYADLLCFSFKEEGSPRVVVWYNEQALTEHF
                     RCKAAGLDTTWDVELSNFTEEVATTFDDFLTLLREHREDLDK"
     gene            523..936
                     /locus_tag="PKZ53_25315"
     CDS             523..936
                     /locus_tag="PKZ53_25315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43826.1"
                     /translation="MDIDQLVAKTQKRLFVSLVTHAVLVTPECLKRKQYKSSFELRFF
                     LHPPNPEHIIQAWKRGSQELEPCAPEEFLAMCKERRKSRLLFCFLEEGNLVYLTWAYK
                     LQGFSRGGSGGTEAYIWTPSGWESVSVPGCGRWIS"
     gene            1064..1297
                     /locus_tag="PKZ53_25320"
     CDS             1064..1297
                     /locus_tag="PKZ53_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43827.1"
                     /translation="MIQAKFSLDQAQLEFVGQYHKFGFKDKSALVRAALAHLQRKLEQ
                     QQLQASADLYAEVYAEEADLQELTESALTGWPE"
BASE COUNT          349 a          285 c          353 g          359 t
ORIGIN      
        1 tccacagagt tggccggttt cttaattttg caactgaaga aacgctaacg ccaccatttc
       61 aagattcctg gcttcactca gatcaagaca ttcgactgga ttggagttgg gactatatcc
      121 agggcattgc caacatctca gaagggtgtg gggtttttct tcggccattt gccattctct
      181 attcaggtcc accccatcct gatgagatgc aggtgaaata tgccgattta ttgtgtttca
      241 gttttaagga agaaggttct ccgagggtcg ttgtttggta caatgaacag gcattgactg
      301 agcattttcg atgcaaggca gcaggactcg atacaacctg ggacgtagag ttgtcaaatt
      361 tcacggaaga agttgccaca acctttgatg attttctgac actcctgcgc gagcaccggg
      421 aagacctgga taaataagcg tggtatagaa ctcagtgtgt agaaaaagaa cgaacggcaa
      481 gctcaggttc tgttgatatt tagggattga gccacaaatc aaatggacat tgatcaactc
      541 gtcgcaaaaa cccagaaacg gctctttgtg agtctggtga cccatgcagt gctggttacg
      601 cctgagtgtt tgaagcggaa acaatataag tcgagcttcg agttacgatt ttttcttcat
      661 cctccaaacc cggagcacat tatccaggcg tggaaacgtg gttctcagga attggaaccc
      721 tgcgcaccgg aagaattcct tgccatgtgt aaggaaagga gaaaatcccg acttctgttt
      781 tgttttctgg aagaagggaa cctggtgtac ctgacctggg cttacaaatt gcagggattt
      841 tcgcgcggtg ggtcgggcgg cacagaggcg tacatttgga ctccgagcgg ttgggaatcg
      901 gtttcagttc caggatgcgg tcggtggatt tcctgatttg tgtgagatta tctctgaaag
      961 attctgagtt tgatgagccg cttgacaatt tatcatccca agccgcaatg cctccgatct
     1021 ggccctggtc caggggcttc atttggaggg gatgtgaggg gttatgattc aggcaaaatt
     1081 tagtcttgat caagcacagt tggaatttgt tggccagtat cacaaatttg ggtttaagga
     1141 caaaagcgct ctggttcgtg ccgccctggc acatttacag cgcaaacttg aacagcaaca
     1201 acttcaagct tctgccgatc tgtatgccga agtgtacgct gaagaggctg accttcagga
     1261 actgactgaa tctgcactca cgggatggcc tgaatgacaa aaatctttga acgagtaagt
     1321 tgatccagat agaaatagct caatgg
//
LOCUS       DAOQDO010002107         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120295_length_1346_cov_2.723470, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002107 DAOQDO010000000
VERSION     DAOQDO010002107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120295_length_1346_cov_2.723470"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PKZ53_25325"
     CDS             <1..1198
                     /locus_tag="PKZ53_25325"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43828.1"
                     /translation="LEYAGVLQTLGLFEAATSQIAEAEALADTLGSSYVLALVALGRG
                     RLYEDQGNLRLALDSGYQGLARFVQQEDLAGILMSLLLVAGIHTRLGHYPRALEQYHQ
                     ALLRAQEQSNSNAEAMVLYGMGMTLHSQGSYGEALNRGHQALELSQRERGQREWVAAR
                     QARTLLGHLYLDMGLWATAREFLQTALISAQSGGYRLSEMHLLALMGRLHFELAVQRA
                     NGKTQVNPLTMSPEVAAELATATEYLDQAYRLAVHLNHPETQARTMLTRGYVLAFKGK
                     LGLALDIAQQVISLIREMERPTLEAQAYIALGNIRFRLGQFAVGMYDRALTLAREINH
                     RRLMWQALRGLADIARAGGDLQQEKIYLLQASELLVQFQAGLPEELHQSAFARERNIL
                     QQVVPM"
BASE COUNT          290 a          330 c          401 g          325 t
ORIGIN      
        1 tcttgaatat gcaggtgttt tgcaaacgct tggccttttt gaggctgcaa ccagtcagat
       61 tgctgaggct gaggccctgg ctgatacgct agggagttcc tatgttttgg cactggtggc
      121 tttaggccgt ggccgattgt atgaagacca ggggaatctt cgactggcgc ttgattcggg
      181 gtatcagggg ctggcccgat ttgttcaaca ggaggattta gctggcatct tgatgagttt
      241 gcttctggtc gcagggattc atacccgact tggtcactac cctcgggcct tggagcaata
      301 ccaccaggct ctgctgcggg ctcaggaaca atcaaactcg aatgccgaag ccatggtcct
      361 ctatggaatg ggaatgaccc tccatagtca aggcagttat ggagaagctc tcaaccgggg
      421 acatcaggcc cttgaactca gtcagcggga acgtggccag cgagaatggg tggctgcccg
      481 gcaggcacgc acgttactgg gtcacctgta cctggatatg ggattgtggg ccaccgcccg
      541 tgagtttttg caaacggcac tgatcagtgc gcagagtggc ggataccgcc tgagtgaaat
      601 gcacctgctg gcgttgatgg ggcgactcca ctttgaactg gcggtgcagc gggcaaatgg
      661 caagacccag gtgaatccgc tcaccatgag tcctgaagtt gccgctgaat tagcgaccgc
      721 gaccgaatat ctggatcagg cctatcgtct ggcagtccac ctcaatcacc ctgaaaccca
      781 ggcccggacg atgttgaccc gtggatatgt tttagctttt aaagggaaac tggggctggc
      841 cctggatatt gcccagcagg tgatttcctt aattcgtgaa atggaacgtc caacgcttga
      901 agcccaggcc tatattgcgc ttggcaacat ccggtttcga ttaggacaat ttgcggttgg
      961 aatgtatgac cgggcactga cgctggctcg tgaaatcaac caccgtcgcc tgatgtggca
     1021 ggcgttgaga ggattggccg atattgctcg ggcaggtggg gatttacagc aggaaaaaat
     1081 ctatttgctt caggccagtg aactcctggt tcagtttcaa gctggacttc cagaggaatt
     1141 gcaccagtca gcctttgcac gagagcgcaa tattttacag caggtggtgc cgatgtagtg
     1201 tcagggttca ggggataggg ttctggggtc aggtcttcga agaaattgtg ttgatgtcct
     1261 tttcgtccct gatgtccttt ttgtcttttt ctcgaagacc cggaacccgg aacccggaac
     1321 ccggaacccg gaacccggaa cccgga
//
LOCUS       DAOQDO010002108         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120461_length_1345_cov_3.468992, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002108 DAOQDO010000000
VERSION     DAOQDO010002108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120461_length_1345_cov_3.468992"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PKZ53_25330"
     CDS             <1..1222
                     /locus_tag="PKZ53_25330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ43829.1"
                     /translation="VDPETASPYVALMFYLKNAEAISNGEMGVETLGVQAVDEFTLRV
                     EMEHPTAFFIKLTAHFMFVPLPEWTIKRHGAQWVEPAKIVTNGPFTLAEHRPNDVIVL
                     RKSATYWDASTVKLNEISFLPVNDAAAMNLYKAGELDVMTSGLIPLPFMKIIQTKQDY
                     CTGAFFSTYYYSINITRKPFDNPLVRKALNLSINKREIAEKLFNGIIQPATMLIPPGI
                     PGYPVLKGPDFNPSEAQRLLAQAGFPDGKGFPKFTIYYNTQESHKQIAETIQRMWKEH
                     LGLTVELQNEEWQTFQARWDRRDFDVCRDVWQGDYLDPTTFLDIYQTENLNNHSGWLD
                     PKFGGLLKQASTESDPAKRLQILAEAENYLIDQMPLIPIYFYTNSFMTKPFVKGYHVN
                     LLDTHQMKFVSIQN"
BASE COUNT          383 a          336 c          311 g          315 t
ORIGIN      
        1 ggttgacccg gaaacagcct cgccctacgt cgctctgatg ttttacttaa aaaatgccga
       61 agcaatcagc aacggcgaaa tgggggttga aacgctcggg gttcaggcag ttgatgagtt
      121 cacgctccgg gttgaaatgg agcatccaac cgcgtttttc atcaagctga ccgctcattt
      181 tatgttcgtc cccttgccgg aatggaccat taaacgccac ggtgcacagt gggttgaacc
      241 agccaaaatc gtcacgaacg gaccttttac actggccgaa catcgtccca atgatgtgat
      301 cgtgctgcgc aaatcagcca cgtattggga tgcatctaca gtgaaactca atgaaatcag
      361 ctttttacca gtcaatgacg ccgcagcgat gaatttgtat aaggccggtg aactggatgt
      421 gatgacgagc gggttgattc cgcttccgtt tatgaaaatc atccaaacca agcaggatta
      481 ttgcactggc gcgtttttca gcacttacta ctattcgatc aatatcaccc gaaaaccctt
      541 tgataacccg ctggttcgaa aggcgttaaa cctttcgatc aacaaacgtg aaattgccga
      601 aaaactcttt aatggaatca ttcaaccggc cacgatgctc attccgccag ggattccagg
      661 gtatccggtg ctcaaaggcc ctgattttaa cccatccgaa gcccaacggt tgctggctca
      721 ggctggcttt ccagatggaa aaggtttccc gaaattcact atctactaca acacccagga
      781 aagccataaa cagattgctg aaacaatcca gcgcatgtgg aaagaacatc ttggattgac
      841 ggtcgaactg caaaacgaag agtggcaaac ctttcaagcc cggtgggacc gacgtgattt
      901 tgatgtgtgc cgcgatgtct ggcagggtga ctaccttgat ccaacgacgt tccttgatat
      961 ttaccagact gaaaatctca ataaccactc tggctggctt gatcccaaat ttggtggact
     1021 gctcaaacag gcaagtactg aatcagaccc ggccaaacga cttcagatcc tggccgaagc
     1081 tgaaaattac ctgattgacc agatgccgtt gattccaatt tatttctata ccaatagttt
     1141 tatgaccaag cccttcgtca aagggtatca cgtcaacttg ctcgacaccc atcaaatgaa
     1201 atttgtgtct attcagaatt agggttcagg gttcagggtt gaaaaccagg gttccggggt
     1261 agaaaaggac agaaaagacg caagtgaccc aaaggacata aattcgaaaa cccggaaccc
     1321 ggaacccgga acccggaacc cggaa
//
LOCUS       DAOQDO010002109         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120484_length_1345_cov_2.886822, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002109 DAOQDO010000000
VERSION     DAOQDO010002109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120484_length_1345_cov_2.886822"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKZ53_25335"
     CDS             complement(<1..264)
                     /locus_tag="PKZ53_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006457229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNJ43830.1"
                     /translation="MNDPILRFSTRVENYVKYRPSYPKAILTMLEQECGLTADSVIAD
                     IGSGTGFSAVLFLDHGNQVYGIEPNLEMRTAGEAYLARYPKFVS"
     gene            complement(429..1193)
                     /locus_tag="PKZ53_25340"
     CDS             complement(429..1193)
                     /locus_tag="PKZ53_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ43831.1"
                     /translation="MGKQFLDKIVLVTGGGSGIGRATALAFAQAGAQVVVSDVSVGGG
                     EETVRLIQEADGKASFLKADVSVVAEVQALIEKIVADFGRLDIAFNNAGIPGDLGKTA
                     ECSIENWERVVAVDLNGVFYCAKFELQQMLKQGEGIIVNMASVAGLVGTPFGAPAYVA
                     SKHGVVGLTRSMALEYARSNIRVNAVCPAFTDTPILDPLRAFDPAMVERFADRQPLGR
                     LGKPEEVAAVVLWLCSPSASFITGAAIPIDGGTTAN"
BASE COUNT          363 a          368 c          322 g          292 t
ORIGIN      
        1 actcacaaac tttggatacc tggccagata ggcttcccca gccgtccgca tttccagatt
       61 gggctcaatg ccatagacct gattcccatg gtctaaaaac aagacggctg aaaatccagt
      121 gccggaacca atgtcggcaa tgacagagtc ggcggtgagc ccacattcct gttccaacat
      181 cgtgagaatt gctttcggat agctgggccg atatttcaca tagttttcca cccgggtgga
      241 aaatcgcagg atcggatcat tcatagaacc tcttttgagt gaatgagcaa tcaaaaagcg
      301 aggagcgagg agtcagaaaa agattttcgg tgaaagtggt ttgaaatttt gctttgggaa
      361 ctgtgacgta cccaaaagct gaaaagtgaa ttcaaggacg aatgggttga ctcctcgctc
      421 ctcgcgtttt aattggctgt cgttccgcca tcaattggaa tggcggcgcc ggtgataaaa
      481 ctggcggagg gtgagcacaa ccacaacacc accgcagcca cttcttcggg ttttccgagc
      541 cgacctagcg gctgacggtc ggcaaagcgt tcaaccattg ccgggtcaaa ggcgcggagt
      601 ggatccaaaa tcggagtgtc agtaaacgcc gggcaaaccg cgttgacccg gatattggac
      661 cgggcatatt ccagcgccat tgatcgggtt aagccaacca caccgtgttt gctggccacg
      721 taggcaggcg ctccaaaggg tgttccgacc aggcccgcga ccgaagccat attcacaatg
      781 atgccttcac cctgtttgag catttgctgt aattcaaact tggcacagta aaaaaccccg
      841 tttaaatcaa ctgccaccac gcgttcccag ttttcaatcg agcactcagc cgtttttcct
      901 aaatcccccg gaatcccggc attgttaaag gcaatatcaa gtcgtccaaa gtcagcgacg
      961 attttttcaa tcaacgcctg aacttcagcc accactgaca catccgcctt gagaaaactg
     1021 gctttcccgt cggcttcctg aatcaaccgg acggtttctt caccacctcc gactgacaca
     1081 tccgagacca caacctgagc gccagcttga gcaaaagcga gcgcagtggc tcgaccaatc
     1141 ccagagccac cgccagtcac caacacgatc ttatctaaaa attgcttccc cattcgataa
     1201 aaaccctttc tgtgtgaatt gtttaggacc aggactttag ggttcagggt tcagggttca
     1261 gggtttataa agacaaaaag gacggaaaga acataaattc gaaaacccgg aacccggaac
     1321 ccggaacccg gaacccggaa cccgg
//
LOCUS       DAOQDO010002110         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120502_length_1345_cov_2.519380, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002110 DAOQDO010000000
VERSION     DAOQDO010002110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120502_length_1345_cov_2.519380"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKZ53_25345"
     CDS             <1..217
                     /locus_tag="PKZ53_25345"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ43832.1"
                     /translation="VKLSVSDIMKQNVITIAPDTSTLEAIRLMRHNRISCLPVIKDNR
                     LVGVVTERDFMNITHVLLEKMLLNEQG"
     gene            complement(342..860)
                     /locus_tag="PKZ53_25350"
     CDS             complement(342..860)
                     /locus_tag="PKZ53_25350"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNJ43833.1"
                     /translation="MQTIFQAGDPEIERLRGVRGWLLFFCIGLVIFKPIWTILQLIFQ
                     TLALTKLGDAVPFEFWLFYFGEATVNVMLMIFGVIAGIWLWQIQPNAVRLAKIFLISQ
                     PLALVLLSLVVGLIPLPDQTKQAIAYQYGQDLFRILMYSLIWLAYFYKSKRVAVTYAP
                     PTEGQFVYLGVS"
     gene            complement(1037..>1345)
                     /locus_tag="PKZ53_25355"
     CDS             complement(1037..>1345)
                     /locus_tag="PKZ53_25355"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ43834.1"
                     /translation="GGSRINLVTQPHYIVERSEDFIRSLAIETPPEPSTPVTGKVTVH
                     ILISESGEVISVTGKNESSAQLTEASRLARQWKFRPYSVEGQPVRIESTLTLNFSHRF
                     "
BASE COUNT          395 a          320 c          303 g          327 t
ORIGIN      
        1 ggtcaaactt tcagtctcag atataatgaa gcaaaacgtc attacaattg ccccagatac
       61 atcaacgctg gaagcgattc gactcatgcg ccacaatcga atcagttgtt tgcccgtgat
      121 caaagacaac cggctggtcg gggttgtcac tgaacgcgat tttatgaaca tcacccatgt
      181 tttgttggaa aagatgttgc tcaatgagca gggctaatac caatttggaa tgaaatcctc
      241 gtgttaggaa acagtcaagt gattcaatca aataaactta gtgaactact gactacaggc
      301 tactgactac aaactggtat aacatctgat tgaacccgtt ttcaggaaac accgagatag
      361 acaaactgtc cctcagtcgg aggggcatag gtgactgcca cccgttttga cttataaaag
      421 taggccagcc agatcagtga gtacataaga atcctgaaca ggtcctgacc gtactgatag
      481 gcaatggctt gtttggtctg atcgggaagt gggattaacc ccaccacaag tgacagtaaa
      541 accagggcta acggttgtga aatcagaaag atcttggcaa gtctgaccgc attgggttgg
      601 atttgccaga gccagatacc agcaatcacc ccaaaaatca tcagcatcac attgaccgtc
      661 gcttcaccga aataaaataa ccaaaattca aatgggacgg catccccaag tttggttaag
      721 gccagagttt gaaaaatcag ttgcaggatc gtccaaattg gtttgaatat gacgagccca
      781 atacaaaaaa acagaagcca tccccgtact ccacgcagtc gttcaatttc cggatcccca
      841 gcctgaaaga tcgtttgcat tgtccacacc cgctttctta gacgaatcga aacgctgatt
      901 cgtcaatcaa tgctgttggt aaaagataca tctggaacgc cttttcacct gcaacataga
      961 acaagagtca gtcggcagaa ccaacctcca gatgagtttt tgacgctgta ttgcccgcaa
     1021 tcctagcact aaatcctcag aaacggtgcg aaaaattcaa tgtcagtgtg ctttcgattc
     1081 ggaccggttg accttcaact gagtaaggcc ggaacttcca ctgtctggcc agacgggaag
     1141 cttccgtcaa ctgagccgaa gattcattct ttccggtgac gctgatgacc tcgccggatt
     1201 cagagatgag aatatgaacc gtcacttttc cggtaactgg tgttgaaggc tcaggcggtg
     1261 tctcaatggc cagcgagcga atgaaatctt cgcttcgttc aacgatgtaa tgtggttggg
     1321 ttaccaggtt tatccgactt ccacc
//
LOCUS       DAOQDO010002111         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_120566_length_1345_cov_1.917054, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002111 DAOQDO010000000
VERSION     DAOQDO010002111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120566_length_1345_cov_1.917054"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKZ53_25360"
     CDS             <1..445
                     /locus_tag="PKZ53_25360"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ43835.1"
                     /translation="LVRELGVALKEGPCLVFSTDLKVRVRSEGPYFYPDVVVVCEEPR
                     FVGPYGDTLENPQLLIEVLSPTTQDYDRGGKFEQYRTISSFVEYLLVAQDRIHIEHHV
                     READGAWRMTETNDVNGQVTLQLVPVTLLVREIYARIMVEGKTND"
     gene            438..773
                     /locus_tag="PKZ53_25365"
     CDS             438..773
                     /locus_tag="PKZ53_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HNJ43836.1"
                     /translation="MTEHHKIIIASDSGVRIRTFSDISDAVSTSFGSAGLILSEHDVI
                     PEFFDLRSGLAGELFQKFTNYRLRLALVVPEPGVYGERFSELAYEHRTHSMIRLVRSQ
                     DEAMAWLNS"
     gene            1108..>1345
                     /locus_tag="PKZ53_25370"
     CDS             1108..>1345
                     /locus_tag="PKZ53_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43837.1"
                     /translation="MNCLKKLTASVLVSCLVSPLLTAETGPILPVGSTSSVQNPSSAG
                     NPVELKVGIPIEHKLSGGEAHSYLYPLKSNEFIDI"
BASE COUNT          343 a          323 c          333 g          346 t
ORIGIN      
        1 tctggttcgt gaactgggcg ttgccctgaa agaaggaccc tgtctggtct tctcgacaga
       61 tttaaaagtc cgggtgcgtt ctgaaggccc ctatttttat ccagatgtgg tcgttgtgtg
      121 cgaagaacct cgatttgtag gcccgtacgg cgacacgctt gaaaacccgc aactcctgat
      181 cgaagtctta tccccgacga cccaggatta tgaccgggga gggaagtttg aacagtatcg
      241 aacaatttcc agctttgttg aatatttgct ggtggctcaa gatcggattc acatcgaaca
      301 tcacgttcga gaagccgatg gcgcctggcg aatgactgaa accaacgatg tcaatggcca
      361 ggttacattg cagttggtgc cggtaaccct cctggttcgt gaaatttacg ctcggattat
      421 ggttgaaggg aaaaccaatg actgaacatc acaaaatcat cattgcgtcc gattcaggtg
      481 tgaggattcg aaccttcagc gatatttctg acgccgtgag caccagtttc ggttccgctg
      541 gactcattct cagcgaacat gatgtcatcc ctgaattttt tgatctccga agcgggctgg
      601 ctggcgaact gtttcagaag tttaccaact accggctgcg gctggcgctg gttgtcccag
      661 aaccgggagt ctatggagag cgattcagcg agctggccta cgaacaccgg acacactcta
      721 tgattcgcct ggtccggtct caggatgaag ccatggcctg gttgaattca taagtgcgat
      781 cttctgtaat ttttgaatag tcaactcatt caatcaaatg gatttagtga gctgctaatg
      841 caaaccaggg tttgaaaaaa gaatggtttt gaacccacga agcgcctggc cgggtgggga
      901 aaaggtgggg aatgacttgt ttcccaaagc tcgaagactc acccagggct acggactgac
      961 gcccagttcc tgggctgaga accaattgcg gttgacaacc ccttgatgga aagcaaagtt
     1021 ttcagctata cactccgttt catcaacccc accccaaact cctactggaa aaagaagtaa
     1081 gcagaaatca aaaaggttta ttttgctatg aattgcctga aaaagctcac agccagtgtt
     1141 ctggtgtcat gcctcgtctc tccattgttg accgctgaaa ctggtccgat ccttccagtg
     1201 ggttctactt cttcagtaca gaatccatca tctgctggca acccggttga attaaaagtt
     1261 gggataccca ttgagcacaa actgagtggc ggagaagctc actcctacct gtatccattg
     1321 aaatcaaatg agtttattga cattc
//
LOCUS       DAOQDO010002112         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121028_length_1342_cov_4.817405, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002112 DAOQDO010000000
VERSION     DAOQDO010002112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121028_length_1342_cov_4.817405"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKZ53_25375"
     CDS             <1..480
                     /locus_tag="PKZ53_25375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43838.1"
                     /translation="AVEGGGFKPGPMGSRGLGQLSYDKGMRILTATQADNVALEDNRL
                     QHGLLTYSLMHDGIEARQADFNPKDRRITLAEWLGYGVVRVPQLYEEIKLGAVTTFAD
                     NSSQNKPQVLVTDAQGRILERNEERQRVVVEQRVQAPGLFDFARRNREVVVWKAGGK"
     gene            complement(601..1254)
                     /locus_tag="PKZ53_25380"
     CDS             complement(601..1254)
                     /locus_tag="PKZ53_25380"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ43839.1"
                     /translation="MSGKRKPMLNFEEYLAFERKVIDRHEFLDGQIYARAGESLSHST
                     ICANLTGELHTQLKGKPCRTLSPNMKVRSGPYLVGSRMTKGMFSYADATVVCDQPLFH
                     DEHQDVLLNPRVLFEVLSPSTENFDRGEKFHRYARHLASLTDYVLISQNQPMVEHFHR
                     QEDGTWIYRVITGLTEFLELPTIDCWIQLSDLYDRIEFPPAEEDLDDSELEDTIEPS"
BASE COUNT          329 a          337 c          351 g          325 t
ORIGIN      
        1 gctgtcgaag gtggcgggtt taaaccaggc ccaatgggaa gtcgtggact gggacaactt
       61 tcctatgaca aaggcatgcg gattttgacc gcgacgcagg ccgacaatgt ggcgctcgaa
      121 gacaatcgcc ttcaacacgg gttgctgacc tattcgctga tgcacgatgg tatcgaagcc
      181 cgtcaggccg atttcaaccc gaaagatcgc cgcattaccc tggccgaatg gcttggatac
      241 ggtgtggtgc gcgttccgca attgtatgaa gagataaaac ttggtgctgt gaccactttt
      301 gccgacaatt catctcagaa taaaccgcaa gttctggtga cagatgccca gggacgaatc
      361 ctcgaacgaa atgaagaacg ccaacgagtt gtggtcgagc aacgggtgca ggcccctggg
      421 ctttttgact ttgcccgccg aaatcgcgaa gtggtggtgt ggaaggctgg cgggaagtga
      481 gaaaatcagg gttccgggtt ccgggttccg ggttccgggt tccgggtttt cgaagcgggc
      541 gcggttcaga gcaacaacgc ccttacgtcc tgtcaccttg tcaccctgtc actttgtcat
      601 ttagctcggt tcaatcgtgt cttcaagctc tgagtcatcc aggtcttctt cagccggtgg
      661 aaactcaatc cggtcataga gatctgagag ttgtatccag cagtcaatcg ttgggagttc
      721 gagaaattcg gtgaggccag ttatgactcg atagatccag gttccatcct cctgacgatg
      781 gaaatgttca accatcggct ggttttggga aatcaggacg taatcagtca ggctggcaag
      841 atggcgggca tagcggtgaa atttctcacc ccgatcaaaa ttttcagtag acggtgagag
      901 tacttcaaag agaactcttg gatttagaag cacatcctgg tgttcgtcgt gaaagagcgg
      961 ttgatcacac acaaccgttg cgtcagcata agaaaacatg ccttttgtca ttcggcttcc
     1021 gacaaggtag ggcccactcc gaactttcat gtttggtgaa agtgtccggc aaggttttcc
     1081 tttgagttga gtgtgaagct cgccggtcag gttggcacaa attgtgctgt gcgaaagact
     1141 ttctcccgcc ctggcataaa tctgcccatc cagaaattca tgccgatcaa tcaccttccg
     1201 ttcaaaagca agatattcct caaaattcag catcggtttt cgctttccac tcataacacc
     1261 ctccacaaaa aagatgagca tgacctgatt cgaaaaccct gaaccctgaa ccctgaaccc
     1321 tgaaccctga accctgaacc ct
//
LOCUS       DAOQDO010002113         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121219_length_1341_cov_3.318818, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002113 DAOQDO010000000
VERSION     DAOQDO010002113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121219_length_1341_cov_3.318818"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKZ53_25385"
     CDS             complement(<1..636)
                     /locus_tag="PKZ53_25385"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNJ43840.1"
                     /translation="MKHSIQLDSLHFLQARDLVLMHGWNATAYQILNPGITYWFSSRK
                     DAVIGYVCHAGVRIVAGAPVCDHQRLVEIVAEFEFEAARAGQRVCYFGAQTRMEQLFR
                     NHPTHSTVLLGAQPAWRPQQWGDIVRTRASLRAQIHRARNKSVSVTEWSPELAHEHPT
                     LRQCLNEWLKNKGLPPLHFLIEPKTLARLFDRRVFVAERDGHIAGFLVTSPI"
     gene            complement(769..1290)
                     /locus_tag="PKZ53_25390"
     CDS             complement(769..1290)
                     /locus_tag="PKZ53_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ43841.1"
                     /translation="MEFQLTHALEILRQTPATLRSLLEGLSPEWIHATEGPETWSPFN
                     IVGHLIHGEETDWISRALIILNQGESHPFEPFDRVAMFERYGHQSLAELLDLFAQHRA
                     ANLEKLISLNLTDEQLALRGQHPAFGSVTLRQLLATWVAHDLSHLAQISRVLAHQYQA
                     AVGPWRAYLSILK"
BASE COUNT          346 a          325 c          374 g          296 t
ORIGIN      
        1 gatcggagac gttaccagga acccggcgat atgaccatca cgttccgcta caaaaacccg
       61 acggtcaaaa agccgcgcca acgtttttgg ttcaattaag aaatggagcg gtggcaatcc
      121 tttatttttg agccactcat tgaggcattg acgcaatgtc ggatgttcat gtgccagttc
      181 gggtgaccat tcggtaacgg aaactgattt gttgcgggcc cgatggattt gggctcgcaa
      241 tgaagcacga gttcgaacaa tatcgcccca ttgttggggg cgccaggcag gctgggcgcc
      301 aagcaacaca gttgaatggg ttgggtggtt acgaaaaagc tgttccatcc gggtttgggc
      361 gccgaaataa cagacccgtt gcccggcccg ggcggcttca aattcaaatt cggctacgat
      421 ctcaactaat cgttggtgat cacacaccgg cgcacctgca acaatccgaa ccccagcgtg
      481 gcaaacatat ccaatgacgg catctttgcg ggatgaaaac cagtaggtaa tcccaggatt
      541 gagaatttgg taagccgtag cattccaccc gtgcatcagc accaggtcgc gtgcctgtag
      601 gaaatgaagg gaatccaatt gtatcgagtg tttcattcta aaggtttgag aaataaaaaa
      661 atccaaagag cccaaatcag tcacaattgc cgatttgccc aaaggctgtc aaccctgcac
      721 caccaaaatc ttcattggag gagagagaat accccagtgc ctggacactc attttaaaat
      781 tgaaaggtaa gctcgccagg ggccgacagc cgcttgatat tgatgcgcca gaacccgtga
      841 aatctgggct agatggctta aatcatgcgc gacccaggtt gccagaagct gccggagcgt
      901 aacgctccca aaagccgggt gctgaccacg aagcgcgagt tgttcatctg tcaggttgag
      961 cgaaatcagc ttctcaaggt tggcggcccg atgttgggca aacaggtcca gtaattcagc
     1021 cagtgattga tggccgtacc gttcaaacat cgccacccgg tcaaacggtt caaacgggtg
     1081 actttccccc tggttgagaa tgatgagggc gcgggaaatc cagtcggttt cttcgccgtg
     1141 gatcaaatgg ccgacgatgt tgaatgggct ccaggtttcg ggaccttcgg tggcgtgaat
     1201 ccattccgga gagagtcctt ccagcaggga acggagcgtg gcaggtgttt gccgcagtat
     1261 ttcaagtgcg tgggtgagtt ggaattccat agtcgtgaag tgagaaagtg aaattggtgt
     1321 cggtcacacc agtgtagcaa a
//
LOCUS       DAOQDO010002114         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121393_length_1340_cov_2.931518, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002114 DAOQDO010000000
VERSION     DAOQDO010002114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121393_length_1340_cov_2.931518"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKZ53_25395"
     CDS             <1..1046
                     /locus_tag="PKZ53_25395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43842.1"
                     /translation="VLGFLAAMVPALLISFPLPTSLAGINRRVQLPGDFAMSVTPNTP
                     RTITPGTTSQYEVRIEGVYGFTQSVNFSAKVTPATTNIGLNFSASAVTPGNGTRLTVT
                     TTGNTPLDEYTLTVTGTSGSLVHSVDVKLTVEQTIAPDFALNFNPGERALLPGGAASF
                     LIQTSPQAGFAQLVNLTADITPADSTIGIALSSNLVNAGDAAMLTLTTTSETPVAGYT
                     ITLTGTSGLLVHTATGTLTVTNQPDTSPPVVTDVAFSKKKVNRKADPHVAIVWYSKDD
                     QKLANHSIVYATDGVNFNTMVVTGLSGGTQSFLWEVSNALPKTKLGVVKVIASDAFGN
                     VGEAASVAKLKIK"
BASE COUNT          324 a          352 c          334 g          330 t
ORIGIN      
        1 ctgtgcttgg ctttctggcg gcgatggtgc cggcgctctt gatttctttt cctttaccca
       61 caagtctggc tgggatcaat cgccgggtgc aactgcccgg agatttcgcc atgtcggtca
      121 cacccaatac accacgaacc attacgcccg gaacgacttc gcaatatgaa gtccgtatcg
      181 aaggtgtcta tggctttacc cagagcgtca atttcagcgc caaagtcaca cctgccacaa
      241 ccaacatcgg attgaacttt tcggcttcag cggtgactcc ggggaatggc acccggttga
      301 cggtgaccac cacgggaaac acgccgcttg atgaatacac cctgacggtc accggtacgt
      361 cggggagtct ggttcattca gtagatgtca aactcactgt cgaacaaacc attgcccctg
      421 attttgcttt gaatttcaat ccaggtgaac gggcgttact gcctggtggc gccgccagtt
      481 tcttgattca aaccagcccc caggccgggt ttgctcaact ggtaaatctc acggctgata
      541 tcactccggc tgattccaca attggaattg cgttgtcttc aaaccttgtg aatgcaggtg
      601 atgcggcaat gttgaccttg acaaccacat cggaaactcc ggtggctggg tacacgatca
      661 ctttgacggg gacctctgga ctgcttgtcc acactgccac tggtaccctc acggtcacaa
      721 atcaacctga cacgtcaccg ccggttgtga ccgatgttgc tttttccaaa aagaaggtga
      781 accgcaaagc tgatccacat gtcgccattg tctggtattc aaaggacgac cagaaactgg
      841 ccaatcattc gattgtgtat gccaccgacg gtgtcaattt caacacgatg gttgtgactg
      901 gcctttcagg aggcacacag tcttttctct gggaagtctc caacgctttg ccaaaaacca
      961 aacttggtgt ggtgaaagtc attgccagcg atgctttcgg caatgtcggg gaagcagcct
     1021 ctgttgcaaa actcaaaatt aaataaaaca gggttccggg ttccgggttc cgggttccgg
     1081 gtcttgtcat tacccaaatt attttggttc gtcaaaaccc acgactgaaa tgaaaaaact
     1141 cgtagaagaa tgacgaagtt cagttctgac cagggcaatt tgggccattt tttagagaac
     1201 tgaaaaaatt tgggtaatga caagacccgg aacccggaac ccggttattg gatcttggat
     1261 ggtgagcgga actggcccgc accagtgccg atgttgtttg gcgaaccaac cagattccac
     1321 tgtccggttg ggataaaccg
//
LOCUS       DAOQDO010002115         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121446_length_1340_cov_2.165759, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002115 DAOQDO010000000
VERSION     DAOQDO010002115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121446_length_1340_cov_2.165759"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PKZ53_25400"
     CDS             complement(<1..951)
                     /locus_tag="PKZ53_25400"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ43843.1"
                     /translation="MHLKTVTLRNFRCFKELKLNLHPRLTVLVAENGGGKSAVLDAIR
                     MGLSLYIDTITDTQQKVKFSHTDIQVFQGNYTEQITFEGDCEIAGQSLHWSQALKSSS
                     KSNRLTSKNTRSLTNAAKALRDQVNNFHVNSGQPVPILSLVVSYGTKRLWIDNLRKEK
                     LRIKRSNNIRLEGYAGCLGSSASVSTIVGWYEARMKEMGSGQYSTETENYTKVIAGIE
                     TAMKVVLEPTGWCELEWDFSKKKLLVKHSVFGSLLLEQLSDGIRTMVVLTMDIARRCS
                     MLNPQFGEDAAQKTPGLVLIDEVDMHLHPRWQQLVLLSLQN"
     gene            complement(965..>1340)
                     /locus_tag="PKZ53_25405"
     CDS             complement(965..>1340)
                     /locus_tag="PKZ53_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HNJ43844.1"
                     /translation="LAMNAVRQAEEARGCRVVDVSAQKCGWDLTSYPPVVDGIQPDAR
                     HIEVKGRVKGATTITVTRNEILYAFNQADKFVLAIVFIGEHEAIDGPYYLTNPFDSEP
                     GWGISSINFNLNQLIARAKRTL"
BASE COUNT          353 a          324 c          285 g          378 t
ORIGIN      
        1 attttgcaaa gagagtaaaa ctaactgctg ccagcgtgga tggagatgca tatccacttc
       61 atctataagc actaaccctg gtgttttttg cgcagcatcc tcaccaaact gcgggtttag
      121 catagagcac cggcgtgcaa tatccatggt aagcacaacc atagttcgaa taccatcact
      181 gagttgttcg agtaataacg aaccaaaaac tgagtgcttt accagtaatt tcttctttga
      241 gaaatcccac tccagctcac accatccagt tggttcaagc accaccttca ttgcagtttc
      301 tatcccagct attacctttg tgtaattctc agtttcagtt gaatattggc cgctgcccat
      361 ttctttcatt ctggcttcat accaaccaac aatagtgcta acagatgcag aggaccccaa
      421 acaaccagca tacccctcta accgaatatt attacttctt tttatcctga gcttttcttt
      481 acgtaggtta tctatccata agcgtttggt gccataggaa accacaagtg acaatattgg
      541 aacgggttga ccgctattaa catggaaatt gtttacttgg tctcgaagtg ctttcgcagc
      601 gttagtcaat gaccttgtat ttttactggt tagccggttt gatttactgg aactcttaag
      661 cgcctgactc caatgaagag actgaccagc aatctcacaa tcaccttcaa atgtgatttg
      721 ctcagtataa tttccttgaa agacctgaat atcagtgtgg ctgaatttaa ctttctgttg
      781 ggtatctgtg atcgtgtcaa tatataagct cagccccatt cgaattgcat caagaactgc
      841 agattttcca cccccatttt ccgccaccag cacagtcagc cgagggtgca aattgagttt
      901 caactccttg aaacagcgaa aatttctgag ggtgactgtc ttgagatgca tgggtatacc
      961 tgggttaaag ggttcttttg gctcgggcga tcagttggtt caaattgaag ttaatcgaag
     1021 atattcccca gccgggctct gaatcaaatg gattcgtcag gtaataagga ccatcaatgg
     1081 cctcatgttc accaatgaaa acaatggcca ggacaaattt gtcagcctgg ttgaacgcat
     1141 agaggatttc attgcgggtc acagtgatgg tggtggcccc tttgacgcgt cctttgactt
     1201 cgatatggcg ggcatccggc tggattccat ccacgacagg cgggtaggac gtgagatccc
     1261 agccacattt ctgtgcggaa acatccacga cacgacaacc acgggcttct tcggcctgac
     1321 ggacagcgtt catcgccagc
//
LOCUS       DAOQDO010002116         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121703_length_1338_cov_3.915043, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002116 DAOQDO010000000
VERSION     DAOQDO010002116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121703_length_1338_cov_3.915043"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..690
                     /locus_tag="PKZ53_25410"
     CDS             178..690
                     /locus_tag="PKZ53_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43845.1"
                     /translation="MSVRHLLMCVLLVFGIGFVTEPTWAQNQGKLPAPKETRPMEPPF
                     RIQWDPENVPPDTKFYQLAANQRIENNGSVIWTVPTLGFREARVVVFMTEAEKIGKVA
                     SPSLRLGTIVRDGDKEFEIDNDSISIETGPGKTSGKRMTMAVYSDSLTLKVEARNLTG
                     NLHFSVYLIK"
     gene            complement(775..>1338)
                     /locus_tag="PKZ53_25415"
     CDS             complement(775..>1338)
                     /locus_tag="PKZ53_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43846.1"
                     /translation="VLLILFPSVSAGAAGFPQVTTPQPTTLRDREVNACSALCVMRNI
                     WGAQREYAKVVGKGNFAPSMDLLGDEEKGIGFLDASLVNMQQTPRLGYVLSAITIQPA
                     TPQSPAKFSIVAYPAIQDGPNRTGDDCFYLDETFVIRHSGSATVFPTAQSEVVPDDLF
                     KTGDWNDLIEEGMKRWEQQMRPSAEKQ"
BASE COUNT          364 a          350 c          306 g          318 t
ORIGIN      
        1 caagcaccaa gcaccaagca ccaggtacca agcaccaggt accaagtacc aggtaccaag
       61 taccaggtac caagcacctg gtaccagccc gtgaaccgaa attcacctgt ttcgcgcgta
      121 cttccaacaa gaaattcacc accaatccac ctttcataat aaaaggagag aatgatcatg
      181 agcgttcgtc acctgctgat gtgtgtcctg ctggtgtttg gaattggatt tgtaactgaa
      241 cccacctggg cccaaaatca agggaagctc cctgcgccca aagagacgcg cccgatggag
      301 ccaccatttc gcatccagtg ggatcctgag aatgtgccgc cggataccaa attctatcaa
      361 ctggcggcca atcaacggat tgaaaacaat ggttccgtca tctggaccgt tccaaccctt
      421 ggctttcgcg aagcccgtgt ggttgttttt atgaccgaag ccgaaaaaat cgggaaagtt
      481 gcgtcaccat cgcttcggtt gggcacgatt gtgcgcgacg gtgacaaaga atttgaaatt
      541 gacaatgact cgatcagtat cgaaaccggt cccggcaaaa ccagtgggaa acggatgacg
      601 atggcggttt attcagattc gctaaccctc aaggtcgaag cccgaaacct caccgggaat
      661 ctccactttt cagtgtattt aatcaaataa aaagcttgga acatggcgaa tggcgaatga
      721 atcggttcaa accgtttcat tcgccattct tcactgagct tgaattttct gaaactactg
      781 cttctccgcc gacggtctca tttgctgctc ccagcgcttc atcccctctt cgatcaagtc
      841 attccaatca ccggttttga ataaatcatc tggtaccact tcactttgtg ccgttggaaa
      901 cacggtggct gatccagaat ggcgaatgac aaaggtttca tccaggtaaa aacagtcgtc
      961 tccggttcgg ttagggccat cctgaattgc tggataggca acgatagaaa attttgccgg
     1021 actctgggga gttgcaggtt gtatggttat ggcactcagc acatagccaa gtcgtggggt
     1081 ctgctgcata ttcacaagac tcgcatcgag aaaaccaatg cctttttcct catcgcccaa
     1141 taaatccata cttggcgcaa aattaccttt accaacgacc ttggcatatt ctctctgggc
     1201 gccccatata ttccgcatta cacacaaggc actacaggca ttgacttcac gatctctgag
     1261 tgttgtgggt tgaggggtag tgacctgggg aaaaccagca gcaccggcac ttaccgatgg
     1321 aaaaagaatc aataaaac
//
LOCUS       DAOQDO010002117         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121716_length_1338_cov_3.097428, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002117 DAOQDO010000000
VERSION     DAOQDO010002117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121716_length_1338_cov_3.097428"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1338)
                     /locus_tag="PKZ53_25420"
     CDS             complement(<1..>1338)
                     /locus_tag="PKZ53_25420"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNJ43847.1"
                     /translation="GSVANAQAAIVTTLNRVTQIYERELAIRLVLVKTFVFSNPETDP
                     FNNDDAGAMIDQNIAFYASSNGIAASAYDVGHVLGVGGGGVALLGSVCDNSSKAGGVT
                     TGAPPVGDPFDVDYVAHEFGHQFGADHTFNAAEGGACTTRNESTAFEPGSGSTIMGYT
                     NICQGRDLQPASDDYFHAGSLKEILELTINGSGSCGQSPNPQAVNNPPRVTVPNPVVI
                     PARTPFALTAVGTDLDAQDAARLTYCWEQYDLGEPGPPDDDNGNRPIFRSYSPSSNPT
                     RVFPQLRYILNNGNTPPETYTVPNNEKVFQTGETLPRTNRVMRFRVTVRDNRVSDGSV
                     AGTVGFGDTQIRVVADAGPFVVTEPVASTVWSTNSRQTVRWNTAFTDQAPINCTSVKI
                     TLSTDGGQTFPATLAENVPNNGTASVVVPDLPLTASVATARVRIESVGNIFFDI"
BASE COUNT          317 a          364 c          360 g          297 t
ORIGIN      
        1 aatgtcaaaa aagatatttc ctactgattc aattcgcacc cgagctgtgg cgacagaggc
       61 ggtaaggggc aagtccggca ccaccacgga agcggtccca ttattcggca cattttcagc
      121 cagtgtggcc gggaaggttt gcccgccatc ggtggaaagc gtgattttta cactggtgca
      181 attgatcgga gcctggtcgg taaaggcggt gttccagcgg acggtttgac gtgaattggt
      241 ggaccagacc gttgaggcaa ccggttcggt caccacaaat ggaccagcat cagccaccac
      301 tcgaatctga gtgtcgccaa atccgaccgt tccagccaca ctaccatcac tcactcgatt
      361 atcacgcacc gttacccgga aacgcatcac ccggttggtt cggggcagcg tttcgccagt
      421 ctgaaacacc ttttcattgt tgggaacggt atatgtttct ggcggagtgt tgccgttgtt
      481 taaaatgtag cgcagttgcg ggaaaacccg ggttggattt gatgaaggtg aataggatcg
      541 gaaaatcggt cggttgccgt tgtcatcatc gggaggcccc ggttcaccta aatcatactg
      601 ttcccagcaa tatgtcagac gggcggcatc ctgggcatcg aggtcagttc caaccgccgt
      661 caacgcaaag ggcgtccggg ctggaatcac aaccggattt ggcaccgtga cgcgtggtgg
      721 attattgacg gcttgcggat tgggcgactg cccgcaactc cccgaaccat tgatcgtgag
      781 ttcaagaatt tctttcagac tcccggcatg gaaataatca tcgctcgccg gttgaaggtc
      841 acgcccttgg caaatgttgg tataacccat gatggttgag ccgctacctg gttcaaacgc
      901 cgttgactca ttgcgggtgg tacaggcacc gccttcggca gcgttaaagg tatgatccgc
      961 cccaaactga tgtccaaatt cgtgggcgac ataatccaca tcaaacgggt caccaaccgg
     1021 tggggcgccg gtggtcacgc caccagcttt gctggagtta tcacacacag agcctaacag
     1081 cgcgacacca ccaccaccaa cccccagcac gtggccaaca tcataggcac tcgcggcaat
     1141 accgtttgaa ctggcataaa aggcaatatt ctgatcaatc atggcgccag cgtcgtcatt
     1201 attgaatgga tcggtttccg gattggaaaa aacaaacgtt ttcaccagca ccagccgaat
     1261 ggcaagctcc cgttcataaa tttgggttac ccggttgagg gtcgtaacaa tcgccgcctg
     1321 ggcattggcc acacttcc
//
LOCUS       DAOQDO010002118         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121770_length_1338_cov_2.310210, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002118 DAOQDO010000000
VERSION     DAOQDO010002118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121770_length_1338_cov_2.310210"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..387
                     /locus_tag="PKZ53_25425"
                     /pseudo
     CDS             1..387
                     /locus_tag="PKZ53_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
     gene            545..1270
                     /locus_tag="PKZ53_25430"
     CDS             545..1270
                     /locus_tag="PKZ53_25430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ43848.1"
                     /translation="MEITLRQVSKRYLLLDALKEVNATIEPGEIVAVLGLNGAGKTTL
                     LQCLAGIVHPTSGEILFDGEHLRRDRMDLRRRFAFMPDMPFLFPDLTPIRHIGMVLKL
                     YEKTYAGIEDRVVDILRGLDLLPLAESRIGTLSRGQAYKVGLAALMAAEPELLMLDEP
                     FASGMDTQGMSAMKLFSREAASRGKTVVYTTQILEVAEAFSHRVMILNRGEVFAFDRL
                     ERLLTMPGDDLSSVFKRLREQRS"
BASE COUNT          314 a          331 c          369 g          324 t
ORIGIN      
        1 aaaaaactgg ggtttgatgt ctgttgcggc ggaatctttg gcatgggtga aacccgcgaa
       61 caacgggttg aactggctgt cacgctccgc gaacttgacg ttgatcacat cccaatgaat
      121 tttctcgacc cgcgaccagg caccccactg gccgacgtcg aacgactttc cccaaccgaa
      181 tgcctgaaaa tcattgccgt gtatcggttg atgatgccca ccaaagatat ctttgtgatg
      241 ggtggccgtg aagtgaacct gcgcgatatg caatcgtgga tctttatggc tggtgccaac
      301 ggcatgctgc ttggcaacta tctcaccacc agaggccggt cagccgaaga cgatctgaaa
      361 atgctcgaag atttaggcat gaaaattaag ttgccagaaa taaaaccggc accaaatgac
      421 acagtcgagc gtccaatgaa gctaacgcaa ctgcgacggg caacaaactg agcgaaggag
      481 cgaaagattg aagtagctca acttcgctcc ttcaatcttt cgctccttca atctgggttt
      541 cgatatggaa attacgcttc gacaggtttc caagcgatat cttcttctgg acgcactcaa
      601 agaggtcaat gccacgattg agccaggaga aattgtagcc gtgctggggt taaatggcgc
      661 tgggaaaacc acgcttctgc agtgtctggc tggcattgtg caccccacca gcggagaaat
      721 tttgtttgac ggcgaacact tacgccggga ccggatggac ctccgacgcc ggtttgcctt
      781 tatgccggat atgccgtttt tgtttcctga cctgactccg atcaggcata tcggcatggt
      841 gttgaaactc tacgaaaaaa cctatgccgg cattgaagac cgcgtggtgg atattttgcg
      901 cgggttggat ttgctgccac tggccgaatc ccgcatcggg acactctcgc gcgggcaggc
      961 atataaagtc ggtttggccg ctttgatggc tgccgagcct gaactcctga tgctcgatga
     1021 accgtttgct tccgggatgg acacccaggg catgtccgcg atgaagctct tttcgcgcga
     1081 agccgcttcg cgtggcaaaa ccgtggtcta tacaacgcaa attctcgaag ttgccgaagc
     1141 cttttcgcat cgggtgatga ttctcaatcg aggcgaagtc tttgcctttg accggcttga
     1201 acggttattg accatgccgg gtgatgattt gtcttctgtg tttaagcggt tgcgcgaaca
     1261 gcggagttag ggttccgggt tctgaaggga tgagggatga gggatgaggg atgaaaaaaa
     1321 tcaccttgtc accttgtc
//
LOCUS       DAOQDO010002119         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121845_length_1338_cov_1.759938, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002119 DAOQDO010000000
VERSION     DAOQDO010002119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121845_length_1338_cov_1.759938"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PKZ53_25435"
     CDS             complement(<1..1285)
                     /locus_tag="PKZ53_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNJ43849.1"
                     /translation="MIGDPVLFAAGGDDHAFHIIYCRLKSDRLLFGDEREIVDTLSAD
                     HPYVLFVFSNQSQSAWHFLNLKYTTKTGIRRVFRRITVQKGEGLRTATERLALLDLEA
                     IQPLSPLAIQQRHDEAFDVEAVTKKFFTTYRDVFQKVEQSIEGTSSIEQRRLFTQRLF
                     NRLMFIAFIQKKGWLRFSHAQTGKGRRQIPASDETPNYLDQLSENYRNDPIVERNFYR
                     ERLKLLFFSGLNTPNEANLVSIANGGFLRKLIGDVPYLNGGLFEEDDDDRNPSIFVPD
                     ASIQLILEDLFAGFNFTVTESTPLDIEVAVDPEMLGKVFEELVTGRHESGSYYTPKPI
                     VSFMCREALKGYLATELPKESPAALEAFVDQHHPADLTDPEAVLEALRRVTVCDPACG
                     SGAYLLGMLHELLDLRAALFAVKQVDNRSNYDRKLE"
BASE COUNT          324 a          341 c          351 g          322 t
ORIGIN      
        1 tttccagctt gcggtcatag tttgagcggt tgtctacctg cttcacggca aagagcgccg
       61 cccgcaagtc cagcaattcg tggagcatgc ctaaaaggta ggccccgctg ccgcaggccg
      121 ggtcacagac cgtcacccgc cgaagtgcct ccagcacggc ttccgggtcg gtcaggtccg
      181 ccgggtggtg ctgatccaca aaagcttcaa gcgcggcagg agattctttg gggagttcgg
      241 tcgccagata gcctttgagc gcctcacgac acataaagct gacgattggc tttggcgtgt
      301 aatagcttcc tgactcatgc cgtccagtaa cgagttcttc aaacactttc cccagcattt
      361 ccgggtcaac ggctacttcg atgtcaagcg gagtgctttc cgtcaccgtg aaattaaacc
      421 cggcaaaaag gtcttccaaa atcagttgaa tggaagcatc tggaacaaaa atgctgggat
      481 tacggtcgtc gtcatcttct tcaaacagac cgccgttcaa ataaggcacg tcgccaatca
      541 atttgcgcag gaagccacca ttggcgatag aaaccagatt ggcttcgttg ggcgtgttca
      601 atccggaaaa gaaaagtaac ttgaggcgtt cacgatagaa attccgctca acgattggat
      661 catttcggta attttccgat aactggtcga ggtaattcgg tgtttcgtcg ctggcgggta
      721 tttgccttct gcccttgccc gtctgagcgt gtgagaatct caaccagcct ttcttttgaa
      781 taaatgcaat aaacatcaac cggttgaaca gccgctgagt aaacagccgc cgttgctcga
      841 tgcttgaagt tccctcgatt gactgttcca ctttttggaa cacgtcccga taggtggtga
      901 agaatttctt tgtgaccgct tcgacgtcaa aagcttcgtc gtgccgttgt tggattgcca
      961 gtggtgaaag cggctgaatc gcctcaagat caagcagggc cagtcgttcg gttgcagtgc
     1021 gaagaccttc acctttttga acggtaatgc gcctgaaaac tcgtcgaata cctgttttgg
     1081 tggtgtactt gaggttgaga aaatgccacg cggattggct ctgattcgaa aaaacaaaga
     1141 gcacataggg atgatctgcc gagagagtat ccacgatctc acgttcatcg ccaaacaaca
     1201 gccggtcaga cttgagccga caataaatga tgtgaaaggc gtgatcatct ccgccagcgg
     1261 caaaaagtac tggatctcca atcagttttt cggcaactcc gggggcggca gttcggagcg
     1321 aaagcggtcg gtcaaccc
//
LOCUS       DAOQDO010002120         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_121953_length_1337_cov_2.768331, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002120 DAOQDO010000000
VERSION     DAOQDO010002120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121953_length_1337_cov_2.768331"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PKZ53_25440"
     CDS             complement(<1..1263)
                     /locus_tag="PKZ53_25440"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HNJ43850.1"
                     /translation="MRSAIILMLGGTGLEVGAMTRRMIVERYGHLRELPIITYLHLDT
                     ALEDASLTPAKVLGVDLSLVSQERIRLEMPSFSDGGVSYLAQHPEIKEWFPSNLRIEH
                     DFSLGAGAVRPYGRIAFAQNAAKIEQALHDCARRVNSNDVRTQVSQRWDPVENGIDVY
                     IVCSLLGGTGGGTFLDAAYLARHVVSRYTPDVQILGYLVIGAGSSIKETPNLANCYSA
                     LKELNYYTTQAQIAKGNINSPFSVEYPGMPFRIESGSVPPFDFCYLVTNFNEENVELQ
                     KPELFELTAQNIFLEFTPGVSSDKRAIRNNIVEHKFRELEQQLGQAQSYLTFGIATIE
                     YPAARVQDCLAYRLAGETVAYLPFYTAPVDPSIPQEAKADLKRWVLDDEQLVASLITG
                     DGGKRITTVIAEQKDQGSAQFQNYLGRPS"
BASE COUNT          314 a          340 c          351 g          332 t
ORIGIN      
        1 cgatgggcgg cccagatagt tttgaaattg ggccgaaccc tggtcttttt gttcggcaat
       61 caccgtggtg atccgttttc ccccatctcc agtgatgagc gatgccacca gttgttcatc
      121 gtcgaggacc caccgtttta aatcggcttt ggcttcctgg ggaattgatg gatcaaccgg
      181 cgcggtataa aacggcaggt aggctacggt ttctcctgcc agccggtatg ccagacaatc
      241 ctgaaccctg gccgccgggt attcaatcgt ggcaatgcca aaggttaagt aggattgagc
      301 ctgtccaagt tgctgttcca gctcacgaaa cttgtgttcg acaatattgt tccgaatggc
      361 ccgtttatcg gatgaaactc cgggtgtaaa ttcgaggaag atgttctggg cggttaactc
      421 aaacagttcg ggtttttgta attcaacgtt ttcttcgttg aagttggtca ccaggtagca
      481 gaaatcaaac ggcggcaccg acccgctttc aattcggaac ggcataccgg gatattcaac
      541 actaaacgga ctgttgatgt tgcctttggc gatttgggcc tgggtcgtgt agtagttgag
      601 ttctttcagg gcgctgtaac aattggccag attgggagtt tctttgatcg aactcccagc
      661 cccgatgacc aggtagccca ggatttggac atctggcgta taccggctga cgacgtggcg
      721 ggccagatag gcagcatcca gaaatgtgcc gcctccggtg ccgcccagga gcgagcacac
      781 aatatagacg tcaatcccat tctcgactgg atcccagcgc tggctgacct gggtgcgaac
      841 gtcgttggaa ttcacccgcc gggcacagtc atggagcgcc tgttcgattt tggctgcatt
      901 ttgggcaaaa gcaattcgtc catagggtct gaccgctccg gcacctaacg agaaatcgtg
      961 ttcaatccgc agatttgacg gaaaccattc tttgatttct ggatgttggg ccagataact
     1021 cacgccgccg tctgaaaaag aaggcatttc aagccgaatg cgttcctgac tgaccagtga
     1081 aagatcaacc ccgagcacct tggctggggt caacgacgcg tcttcaagcg ctgtatcgag
     1141 atgcaaatag gtgataatcg gcaattcccg caaatggcca tagcgttcga caatcatccg
     1201 ccgggtcatt gccccaactt ccagccccgt tccgccaagc atcaaaatga tggctgatcg
     1261 catagagtgc tccttcacag tgcaataatg aattacgaat tataccaatt tgcaatgaag
     1321 ctcttgtatg agaaaat
//
LOCUS       DAOQDO010002121         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122305_length_1335_cov_2.390625, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002121 DAOQDO010000000
VERSION     DAOQDO010002121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122305_length_1335_cov_2.390625"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1335)
                     /locus_tag="PKZ53_25445"
     CDS             complement(23..>1335)
                     /locus_tag="PKZ53_25445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43851.1"
                     /translation="LRATPDLAEVPILMLTALDDHESRVKGIESGADDFLTKPISPDE
                     LNARVHTIVRLNRYRRLHAERAKFDWVVEQSEDGYLVIDDQDQITYANPKACLFLSID
                     PIGTHRDPIYFRPTVQKQYLCQPPELWATWPVESRGADSASASARYLVRPETESSQAF
                     WLQVLQFQEPIQQAARQILCLKDVTSQILTMRDVQAFHRLIPHKLRSPLNSMRLGIEM
                     LAMHSAALLGGEIADLVDMALQGIYRLDQEVTDVVQYLHAPTLARKGKEVPVEGVLPL
                     INRISEELDLLPPLVSISPHLRPYQLQLSEPALERVVFELLENSKKFHPRQLPQITVE
                     AEPAGLEQIKLRITDDGVHLSPEQLERVWTPYYQGEKYFTGQVAGMGLGLPMVAMLIW
                     AAGGSCVLRNRPDQPGIQVELVFPISPSQAKSEDDPVSANPRQP"
BASE COUNT          299 a          342 c          384 g          310 t
ORIGIN      
        1 tgaaaaaagc cggggtcacc ggctatggtt gacggggatt ggcactgacg gggtcgtctt
       61 cagatttggc ttgagatggc gagatgggga acactaattc aacctggatg ccgggctggt
      121 ctggccggtt tcgcaagaca caactgccgc ctgccgccca aatcaacatg gccaccattg
      181 gcaaccccag acccatcccc gccacctggc cggtaaagta tttttcacct tgataataag
      241 gtgtccagac tcgctctagt tgctccggcg ataagtggac gccatcatca gtaatgcgca
      301 atttgatttg ctccagaccg gcgggctctg cttcgaccgt aatttgtggg agctggcgcg
      361 ggtgaaattt ttttgagttt tcaagcagtt caaacaccac tcgctccagc gctggctctg
      421 aaagttgtaa ttgatagggg cgcaggtgcg gggaaatgct taccagtggc ggcaagaggt
      481 caagctcctc actgatccgg ttgatcaggg gcagcacccc ttcaaccggg acttctttcc
      541 ctttacgggc cagggtcgga gcatgcaggt actgcaccac atcggttact tcctggtcca
      601 gtcggtagat gccttgcagg gccatatcaa ccagatcggc aatttcccct cctaaaagag
      661 ccgctgaatg catggcgagc atctcgattc cgaggcgcat ggaattgagc ggagagcgga
      721 gtttatgtgg gatcaaccgg tgaaaggcct gaacatcccg catcgtcagg atttgagagg
      781 tcacgtcttt caaacacaga atctgccggg cagcctgctg gattggttcc tgaaattgca
      841 aaacctggag ccaaaacgcc tgggacgatt cggtttcagg tcggaccagg tagcgcgcag
      901 aggctgaggc ggagtctgcc cctcgacttt ctactggcca ggttgcccag agttcaggcg
      961 gttgacacag gtactgtttt tgaactgttg gccgaaaata gattgggtcc cggtgggtac
     1021 cgattgggtc aattgacaga aacaggcagg ctttggggtt ggcataggta atttgatcct
     1081 gatcgtcaat caccagatag ccgtcctcgg attgttcgac cacccaatca aattttgcgc
     1141 gttcagcgtg cagccggcgg tagcgattca accggacaat ggtatgaacc cgggcattga
     1201 gttcatccgg tgaaattggt ttggtgagaa aatcatccgc gccagattca atccccttga
     1261 cccgcgattc atgatcatca agggccgtga gcattaagat cggaacttcg gccagatcgg
     1321 gtgtcgctcg cagtt
//
LOCUS       DAOQDO010002122         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122335_length_1335_cov_2.120312, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002122 DAOQDO010000000
VERSION     DAOQDO010002122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122335_length_1335_cov_2.120312"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1158)
                     /locus_tag="PKZ53_25450"
     CDS             complement(64..1158)
                     /locus_tag="PKZ53_25450"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ43852.1"
                     /translation="MEVAVIGAGLAGLTTAFELKQQGIGCEVIEKSRTVGGRMNSRRV
                     ADCVIDDGAQYFTVKSPEFAAFLRQIGLADRLHRLAAPVVAYPFSYLDEALAAALEDE
                     ARMANSGLEYPFRYSFAEGMDALPTALAEQLDPEHLNLQTYAEGIRWNVDQQVWDLHT
                     RGDNTTLGGQVSARSVVISLPAPQAAQLLKRSFPLPAPLAELAEALDQVTYYPCLTVL
                     LRMIRDYQLQSIGGLRAEDYAHPIGWMAWIERLVPGRMPESETGLVIQLTPNVSERAM
                     ELPDDQILSATIGSLEQEFGMSLPTVRWIHIKRWRYANPTGVTLDPALREAAEKLGIY
                     LCGDYLERGRIESAFWSGRKLARRIVEMTR"
BASE COUNT          358 a          370 c          337 g          270 t
ORIGIN      
        1 gaccttttga cagggtgaca aggtgacaag agaagtctgt caaaaggtca ctttgtcaaa
       61 aggtcatcgg gtcatctcaa caatccgccg ggccagtttt ctgcccgacc aaaaggctga
      121 ttcaattctg ccgcgctcta aataatcgcc acacagatag ataccaagct tctcagccgc
      181 ctcgcgtaag gctggatcaa gcgtgacacc agtcggattg gcataccgcc agcgtttgat
      241 atgaatccag cgaacagtgg gcaaactcat tccaaattcc tgctctaatg aaccaatggt
      301 cgcactcagg atttgatcat ccggcaattc catggcccgt tcactgacgt ttggcgtgag
      361 ttgaatcacc agtccagttt cagactctgg cattcgacct ggcaccaacc gctcgatcca
      421 ggccatccag ccaattggat gggcataatc ctcagctcgt aaaccgccga tactttgaag
      481 ttgataatcc cgaatcatcc gcaacagcac cgtaagacag gggtaatagg tcacctgatc
      541 cagggcttcg gccagttccg ctaaaggtgc cggaagcgga aagctgcgct tgagcaattg
      601 agcagcctga ggtgccggca aggaaatcac gactgaccgg gcactgacct gaccgccaag
      661 cgtggtgtta tcacctcgcg tgtgtagatc ccaaacctgc tgatccacgt tccagcgaat
      721 gccttcagca taggtttgga ggtttaagtg ctctggatcc aactgttcag ccagagcggt
      781 cggaagtgca tccattcctt cggcaaaact gtatcgaaac ggatattcca gccccgaatt
      841 ggccatgcgg gcttcatctt ccagagccgc cgccagggcc tcgtccagat atgaaaacgg
      901 ataggccacc accggtgcag ctaaccgatg aagccgatca gccagcccga tctggcgcaa
      961 aaaggcagca aattcaggtg atttgacagt gaaatactgg gcaccatcat caatgacaca
     1021 gtcggccaca cgacgggagt tcatccggcc cccaaccgtg cggctctttt cgatgacttc
     1081 acatccaata ccctgttgtt tcaattcaaa ggcagtggtt agcccagcta acccagcgcc
     1141 gatgacagca acttccatat gtgttgtgac cagtctgtaa gaagatggac gaacaaatcg
     1201 aggataggaa ccagggttca gggttcaggg tctgaaaacc agggttcagg gtttaaaaga
     1261 ccagaaggac gaaaagaacg aaagttcgaa aacccggaac ccggaacccg gaacccggaa
     1321 cccggaaccc ggaac
//
LOCUS       DAOQDO010002123         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122427_length_1334_cov_3.086787, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002123 DAOQDO010000000
VERSION     DAOQDO010002123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122427_length_1334_cov_3.086787"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PKZ53_25455"
     CDS             complement(<1..866)
                     /locus_tag="PKZ53_25455"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNJ43853.1"
                     /translation="MGSDVPDIANLTPEQRRALLKKLLEIQTRPRTEISPLAHGQRAL
                     WFLHQLAPDSSAYHVISALRIISPVNPAALRQSFQKLVDRHPVLRSTFEIHDTDPVQI
                     IHPHQIVHFTEIEAATWSEDDLERQVRQAAHHPFELSQGPLLRVSLYHRSEIDHLLLV
                     VLHHIVIDFWSLTILVDELRQFYAAEIRGHPAQLPPIQSSYADFVQWQTQWLTSAAGQ
                     TAWTYWSHKLTGEIPTLSLITDQPRPRTQTYRGATVEAIVSVEHTRRLHHFARSEQTT
                     LFAVLLAVFQVLL"
     gene            complement(856..>1334)
                     /locus_tag="PKZ53_25460"
     CDS             complement(856..>1334)
                     /locus_tag="PKZ53_25460"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNJ43854.1"
                     /translation="KRESYFAASVDDRTHLLEGYLQNAIARTLGIKPSRVERFTSLMS
                     LGFDSLMAVTLRSQIESDLGVVLPVASFLQEASVATLTTTLHQSLTALPAPATVKITA
                     LTLEPPPVTPPASVNHNQAPRGTTDDLLDKVDQLSDEQVKALLQQMLSSGGSSHGQ"
BASE COUNT          314 a          306 c          392 g          322 t
ORIGIN      
        1 agcaggactt gaaatacagc cagcaacact gcaaacagtg tggtttgttc agaccgggca
       61 aagtgatgca gccgtctggt atgttcaacc gaaacgatgg cttcaactgt tgcgcctcga
      121 taggtttgtg tccgtgggcg tggctgatcg gtgatgagag aaagggttgg tatctctccc
      181 gtcagtttgt ggctccaata ggtccaggcg gtttgtcctg ccgctgatgt cagccattgg
      241 gtttgccact gtacaaaatc agcataactg gattgaatgg gcgggagttg agccgggtga
      301 cctctgattt cggctgcata gaactggcgt aattcatcaa ccagaatcgt caatgaccag
      361 aaatcaatca caatatggtg caggaccacg agcaacaggt gatcaatttc agagcggtga
      421 tacagactga ctctcagtaa cgggccctgg ctcaactcaa atggatgatg ggcggcctga
      481 cgtacctgac gttcaaggtc atcttcagac caggtggcag cttcaatttc agtaaaatgg
      541 actatttggt gagggtgaat gatctgaact gggtctgtgt cgtggatttc aaacgttgat
      601 cgcaggaccg gatgccggtc aaccagtttc tgaaaagatt ggcggagggc ggctggattg
      661 actggggaga taattcgcag ggctgagatg acgtgatagg cactgctgtc cggcgccagt
      721 tggtgcaaaa accacagtgc ccgttgacca tgcgccaatg gagagatttc tgtgcggggt
      781 ctggtttgaa tttcaagaag tttttttaac agtgctcgtc tttgctcagg ggtcagattg
      841 gcaatatctg gcacatcact gcccatgcga cgatcctcca ctggagagca tttgctgaag
      901 aagcgctttg acctgttcgt cagaaagctg atcaacctta tcaagcagat catccgtcgt
      961 accgcgtggg gcctgattgt gattgactga ggctggcggt gtcacgggtg gtggttccaa
     1021 ggtcagggcc gtgatcttta ccgttgctgg agccggaagc gccgtcaatg actggtggag
     1081 cgttgtcgtc agcgtggcga cagatgcttc ctgcaggaat gacgccaccg gaaggaccac
     1141 gcccagatca ctttcaatct ggctgcgaag ggtaacggcc atcaatgagt caaatccaag
     1201 tgacatcagc gaagtaaatc gctcaacccg tgaaggttta atccccaggg ttcgggcgat
     1261 ggcattttgg aggtaccctt ccagcaggtg tgtccgatca tcaactgagg cggcaaaata
     1321 cgactcccgt ttta
//
LOCUS       DAOQDO010002124         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122428_length_1334_cov_3.044566, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002124 DAOQDO010000000
VERSION     DAOQDO010002124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122428_length_1334_cov_3.044566"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1334
                     /locus_tag="PKZ53_25465"
     CDS             <1..>1334
                     /locus_tag="PKZ53_25465"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ43855.1"
                     /translation="VMVWMSGADGKSFFFNDRWLSFRGRTINEERGDGWLDGVHPDDA
                     QHCFDIYLRAFRQQQEFEIEYRLQRADGEYRWVLDIGRPHYVAGSFAGYVGSCIDITD
                     RKQTERWEAGQKDILELITKNAGLDQIFHLLIKTLEDIIPGMIGSILLLDNDGKRLLQ
                     GAAPHLPDEYNQQIHGLEIGPQVGSCGSAVFQRELIIVEDTFTDPRWAQFEAAARKFN
                     LRACWSQPILSAAGRVLGTFAMYYRTRRQPTTEEQNLIIGAGRLAGIAIERHQAETAI
                     RVSEERLELAVRGANLGLWDWNYETGEIFINDRWAEMLGYERQELDMHQDTWERLVHP
                     DDFPSTQQLLNEHLEGKIPFFQAEYRLRTKSGDWCWVLDSGKVVERDLAGKPRRVSGT
                     HLDITKRKQFEEHLLQSHNLTSIGRLAGGIAHNFNNLLTVVTGYTDLLAKRMP"
BASE COUNT          330 a          324 c          360 g          320 t
ORIGIN      
        1 gtcatggtct ggatgtctgg cgccgatggc aaatccttct tttttaatga tcgctggctc
       61 agttttcgcg ggcgaacaat caatgaggaa cgtggcgatg gttggctgga tggggtgcat
      121 ccagacgatg cccagcactg ttttgacatc tatcttcggg cgtttcgcca gcaacaagaa
      181 tttgaaatag aataccgcct tcaacgagcc gatggtgaat accgctgggt tttggatatt
      241 ggacgaccac attatgtcgc gggaagcttt gcgggctatg ttggaagctg cattgacatt
      301 acagatcgaa aacaaaccga acgctgggaa gcaggtcaaa aagacatctt agaactcatt
      361 accaaaaatg ctggcctgga tcagattttt cacctgttga tcaaaaccct ggaagacatc
      421 attccaggca tgatcggctc tatcctgctc ctggataatg acgggaaacg actcttgcag
      481 ggtgcggcgc cacacctgcc tgacgaatac aaccagcaaa tccatggcct tgaaattggt
      541 ccccaggttg ggtcgtgcgg aagcgctgtt tttcagcgag aactgatcat tgttgaagat
      601 acattcactg accctcggtg ggctcaattt gaggctgcag cccggaaatt taacctgcgg
      661 gcctgctggt cccaaccaat tctttcggcg gctggtcgcg tgctgggcac atttgccatg
      721 tattaccgca cccggcgcca gccaaccacc gaagagcaga acctgattat tggtgctggt
      781 cggctggctg gaattgctat cgaacgtcat caggcggaaa cggcaattcg ggtgagcgaa
      841 gaacggctgg aactggctgt ccgtggagca aaccttgggt tgtgggactg gaattatgaa
      901 accggggaaa tttttatcaa tgaccgctgg gccgaaatgc ttggttatga gcgtcaagaa
      961 ctggatatgc accaggatac ctgggagcgc ctggttcatc cagatgattt tccttctacg
     1021 caacaactcc tgaatgagca tctggaggga aaaatacctt ttttccaggc cgaatatcgt
     1081 cttcggacca aatctgggga ctggtgctgg gtcctcgaca gtggaaaagt ggtggaacgt
     1141 gacctggcgg gaaaaccacg tcgggtttct ggcactcacc ttgatatcac caaacgcaaa
     1201 caatttgaag aacaccttct gcagtcacac aacctgacca gcattgggcg gctggcaggc
     1261 ggtattgctc ataatttcaa caatttgctc acggtcgtta caggctacac tgatttgctc
     1321 gccaaacgaa tgcc
//
LOCUS       DAOQDO010002125         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122823_length_1332_cov_2.704777, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002125 DAOQDO010000000
VERSION     DAOQDO010002125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122823_length_1332_cov_2.704777"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="thrC"
                     /locus_tag="PKZ53_25470"
     CDS             <1..1025
                     /gene="thrC"
                     /locus_tag="PKZ53_25470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNJ43856.1"
                     /translation="LNPTGSFKDNGMTTGTTQANRMQAKAVACASTGNTSASMAAYAA
                     RAGMKGIVFIPGGQIAFGKLSQSMDYGAVTLQIDGDFDAAMRIVQEIARETDIYLLNS
                     INPFRLEGQKTIAFELLQQLEWQVPDRVVLPGGNLGNSSAIGKGLKELHSLGLIDRLP
                     KITIVQAEGASPLAQYWASPSGTPFKAVEKAFTLATAIKIGNPVSWAKAIRALEWTNG
                     TCISVSEQEIADAKAIIGRDGIGCEPASATTVAGMRKMVTAGHMDPDEVVVGILTGNV
                     LKDPDYTIQYHLGELYQRSERRTEIVKADSPLHSTFANQPIQVAADKKEIYKVLEHIL
                     AEEHNN"
     gene            1033..>1332
                     /locus_tag="PKZ53_25475"
     CDS             1033..>1332
                     /locus_tag="PKZ53_25475"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ43857.1"
                     /translation="MFGLSFTELVFIFFVALLIFGPRKLPEIGRTLGKMMNQFRQASN
                     EFKRAWEAEVDLESTRQALQAVKDPIGAIVPAVTASLTAPLDPAPAAVSLTKAEPP"
BASE COUNT          349 a          343 c          338 g          302 t
ORIGIN      
        1 ggttaaaccc aactggttca ttcaaagaca atggcatgac cactggtaca acccaggcaa
       61 accggatgca ggccaaagcc gtggcctgtg ccagtacggg caatacctcg gcatcaatgg
      121 ccgcctatgc tgcccgcgct ggaatgaaag gaatcgtgtt tattcccggc ggccagattg
      181 cctttggcaa actctcacaa agcatggact acggcgcagt taccctccaa attgatggcg
      241 attttgatgc ggccatgcgg attgtccagg aaattgcccg ggaaacggac atctatttat
      301 taaattcgat taacccattt cgattggaag gccaaaaaac catcgccttt gaactgctcc
      361 agcaactgga gtggcaggtt ccagaccggg ttgtgttgcc aggaggaaat cttgggaata
      421 gttcggccat tggcaagggt ttgaaggagt tgcactcact gggtttgatt gatcgccttc
      481 ccaaaatcac cattgttcag gctgagggtg ccagcccgct ggctcagtac tgggccagtc
      541 catctggaac gcccttcaaa gcggtcgaaa aggcatttac cctggcaacc gccattaaaa
      601 ttggcaaccc ggtttcatgg gcaaaagcca tccgggctct tgagtggacc aacggcacct
      661 gtatttctgt gtcagaacaa gaaattgcag acgccaaagc aattattgga cgcgatggca
      721 tcggctgtga accagcatca gccactacag tcgccggaat gcgaaaaatg gtcacggctg
      781 gacacatgga ccccgatgaa gtggtcgtcg gcatcctgac gggaaatgtg ctcaaagacc
      841 ctgactacac gatccaatat cacttaggag aactttatca gaggtccgaa cgtcgaactg
      901 aaatcgtaaa ggctgattct ccccttcatt caacctttgc caaccagcca atccaggttg
      961 cggcagacaa aaaagagatt tacaaggtgc tggaacatat cctggcagag gaacacaata
     1021 actgaggtcg ctatgtttgg tttaagtttt acggagctgg tgtttatttt ttttgtagca
     1081 ttgttgatct ttggtccacg caaattgcct gaaattggtc gtacgctggg aaaaatgatg
     1141 aatcaatttc gacaggcatc aaacgagttc aaacgcgcct gggaagctga agttgatttg
     1201 gaatccaccc ggcaagccct ccaggccgtg aaagatccaa tcggggccat tgtcccggca
     1261 gtgaccgctt ccctcaccgc tccgttagac cccgctccag cagccgtatc gcttacgaaa
     1321 gcagaacctc cg
//
LOCUS       DAOQDO010002126         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122826_length_1332_cov_2.695380, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002126 DAOQDO010000000
VERSION     DAOQDO010002126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122826_length_1332_cov_2.695380"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKZ53_25480"
     CDS             <1..718
                     /locus_tag="PKZ53_25480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNJ43858.1"
                     /translation="HVGNRQTKPMVDGKQVATPKQPLTMAEWERIAVVVIFFMFTMFF
                     WAAYEQKGTSLNLFAKELVRTEIFGWSFPSSWLQSMTAFYVILLAPVFSKVWVALGDR
                     QPSSPVKFIFGLLFIGCGYLLMIPAAMLTASGKISPLWFVALYFLEVMGEMCLSPVGL
                     STVTKLAPARVLGIMLGVWFLASAFGNKLAGYLAGFYDASNAPVLTTLYGGIAVGLFV
                     AAGILATLTPTIKRMMGDVK"
BASE COUNT          328 a          294 c          335 g          375 t
ORIGIN      
        1 tcatgttgga aaccgacaaa ccaaacccat ggtggatgga aaacaggtgg caacgcccaa
       61 gcaacccctg acgatggctg aatgggaacg aattgcggtg gttgtgatct tttttatgtt
      121 cacaatgttt ttctgggcgg cctatgagca aaagggaacc agtctgaacc tgtttgccaa
      181 agaactggtt cgaactgaaa tatttggctg gtcattccca tcttcctggc tgcaatcaat
      241 gacggccttt tatgtgattt tactggcgcc cgttttttcc aaagtgtggg ttgcgttggg
      301 ggatcggcag ccttcaagcc cagtgaaatt tatttttggg ctcttgttta ttgggtgcgg
      361 gtatttattg atgattcccg ccgccatgct caccgcgagt ggaaaaatca gtccgctctg
      421 gtttgtggcg ctctactttt tggaagtgat gggtgaaatg tgtttaagcc cggttggtct
      481 gagcacggtg accaaactgg cgcccgcacg ggttttgggg attatgctgg gcgtatggtt
      541 tctggcatca gcatttggta acaagctggc cggctatctt gccgggtttt acgatgcctc
      601 aaacgctcca gtcctgacca cattatatgg cgggatcgca gtggggcttt tcgtcgcagc
      661 cggcattctt gccacgctca cgccaacgat taagcggatg atgggcgatg tgaagtaatt
      721 tttaattaaa atttaaattg tggtttaatt aaaatttaaa aaacagggca ccgtggctga
      781 atactcagcc acggtgcccg ttgtttttta tcgtccaaat gaaaaaaatt gaccgtgagt
      841 gaagcaacct tttgtccacc agatcaatct ctggttccat ttttcggttg ccagaccgtc
      901 cctagtgtaa cggattttgg cagttaccat atctgtatct aagggttaca cccaccgatc
      961 agcactgaat tggagggggg acgccggaaa aatccggcac atcagcacaa tttttgccaa
     1021 actttcgggc acaaaacagt tgctcgggcg tctaatgaat caactccaaa gtacatttct
     1081 ttccaaatta agccgtagtt ccaatgtttt ttgcttgtgg tatgcaagtt gctctcattt
     1141 gaagcaactg cattgattta ttccctcaac aagagaaaag aaggagaaga tcgtatggta
     1201 gaagtgaatt tactcagaag tcagtctgta aaggatcgga aattggtatc ggccaggccc
     1261 tctgtccagc cagcatctga ctcagccgcg atgacgccag gcactatttt aaccaaactg
     1321 atggccgaac tg
//
LOCUS       DAOQDO010002127         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122890_length_1332_cov_2.163665, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002127 DAOQDO010000000
VERSION     DAOQDO010002127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122890_length_1332_cov_2.163665"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..>1332)
                     /locus_tag="PKZ53_25485"
     CDS             complement(298..>1332)
                     /locus_tag="PKZ53_25485"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ43859.1"
                     /translation="KSISLMMVCEMTCVFLVCGLGMLAIAQFLPFFGKVSGQFPSLSL
                     ALAFIGCILVSSPAVTISMMNEYRPTGPVSQTTLGIVVLKDIVVVLIFGLTLALTKML
                     SLSDAQVSITGLAIHFVKELGLSVLFGLVAGLIVSIYLKYIRTNLVFFTVGLSLFAFH
                     VAKMLHLDPVLLCLVAGFFVENFTRQGERFLENLEHTFSVVFVIFFTVAGAKLNLTAL
                     ATVWPIAILFVILRMVSLYAGTQLGAKLSNASPEVARLTWLGLIPQAGVALGLVIIVE
                     QTFPGWGADLATIIVAMIGIHETLGPLAFRLALSMSGEMNREQITHDQKMLQEWDAST
                     SPEMNPNAIW"
BASE COUNT          349 a          364 c          330 g          289 t
ORIGIN      
        1 ggtttgtttg atttgggcgc cgatttctgc cgcctgttcc tgttcgacgg gatcaagttg
       61 attggcgtgg cgcatgactt cggcccggac ttcgccgcga acttcatttt tcaccgcttc
      121 gagttgggcg attcgattgg agtcatcaag catcaattta tcttgttcag tcatggtttt
      181 ttcctcctta aatatctggg ttcgtgatcg gcaagggttc aagatcgcca cttgcgaaac
      241 cacatcctgg ctcctgtacc ctgaacgcta acccctgaac ccgcttacga ttgggggtta
      301 ccagatggca tttggattca tttccggtga tgtcgaggca tcccactcct ggagcatctt
      361 ctggtcgtgg gtgatttgtt cgcgattcat ctcgcccgac atcgagagcg caagccggaa
      421 ggcgagcggt cccagcgttt catgaattcc aatcatggcc acgataattg tagccaggtc
      481 ggcaccccaa ccagggaacg tttgctccac gataatcacc aaccccagcg cgactcccgc
      541 ctgggggatt aaccctaacc aggtcagtcg ggctacttcc gggctggcat tggaaagttt
      601 ggcaccgagt tgagttccgg cataaagact gaccatgcgc aagatcacaa acaaaatggc
      661 aatcggccac accgttgcca gggcggtcaa attcaacttc gcaccggcca ccgtaaaaaa
      721 gatcacaaac acgacgctga aggtatgctc caggttttcc aaaaaccgtt cgccttgccg
      781 ggtaaagttc tcaacaaaaa aaccggcgac caggcaaagc aagaccggat cgaggtgcag
      841 cattttggcc acgtgaaacg caaacagtga caggccaacg gtaaaaaaca ccaggttggt
      901 gcggatatac ttgaggtaaa tcgaaacgat caacccggca accagaccaa acaggactga
      961 aagccccagt tctttgacaa aatggatcgc cagaccagtg atacttacct gggcatcgct
     1021 caaagaaagc attttggtca atgccagtgt gaggccaaag atcaggacca cgacaatgtc
     1081 ttttaacacg acaatcccga gcgtggtctg gctcaccggc ccagttggcc gatattcgtt
     1141 catcatgctg atggtgaccg ccggtgaact caccaaaatg cacccgatga aagccagcgc
     1201 cagcgaaagt gaaggaaatt gcccgcttac tttcccaaag aacggcagaa actgagctat
     1261 cgccagcatt cccaggccac agaccaggaa gacacaggtc atttcgcaaa ccatcatcaa
     1321 ggaaatagat tt
//
LOCUS       DAOQDO010002128         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_122997_length_1331_cov_3.033699, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002128 DAOQDO010000000
VERSION     DAOQDO010002128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122997_length_1331_cov_3.033699"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>1331)
                     /locus_tag="PKZ53_25490"
     CDS             complement(106..>1331)
                     /locus_tag="PKZ53_25490"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ43860.1"
                     /translation="LVMLDITMPEMDGYEVCQQLKADPRSRDIPVIFISALDEVMDKV
                     RAFEVGGVDYVTKPFQFEEVLARIENQLKISRLQKDLETKNAELARKNEELTRSREDL
                     MESNRRAQMIFSALSEVLPGTVLDGKYRLEKKIGTGGFGTVYEATHTGLNRLVAVKVF
                     QPRSGHATQSDLDRFRLEGVSACRIQHPNAVSILDFGISSAGIAYLVMELLSGHPLTK
                     EISQQGRLAPERCVEIALPICEVLAEAHAAGIVHRDIKPDNIFLHQTKDGEVVKVVDF
                     GLAKLLGESRIDLQHVTIGRVIGTPTYMSPERLGNLPYDGRTDIYSLGITLYQMLCGH
                     VPFQSLEGDICTVAVMHLTKEARPLRDIIPGIPPELNDLVMRMLSKSPDRRPSATELC
                     YELKHIRLHPVGNYA"
BASE COUNT          324 a          359 c          322 g          326 t
ORIGIN      
        1 caagaaccaa gaaccaagaa ccaagaacca agaaccaaga accaagaacc aaacaatttc
       61 ttcattcttc attcttcatt cttcattctt cattcttcat tcgtttcagg cgtagtttcc
      121 gactggatgc aaccggatat gctttaactc atagcataac tcggttgcgg acggtcgccg
      181 gtcaggagat ttagacaaca tccgcatcac cagatcattc agttcaggtg gaatgccggg
      241 gatgatgtcg cgcaacggtc gagcttcttt ggtcaggtgc atgacggcca ccgtgcaaat
      301 atcgccttcg agggattgaa acggcacatg cccacataac atttgataca gcgtaattcc
      361 caggctgtag atatcagtcc gaccatcata gggcaggttt ccaagccgct cgggtgacat
      421 ataggttggc gtcccaatca cccggccaat cgtgacatgc tgaagatcaa ttctggattc
      481 cccgagcagt ttggccagtc caaaatcaac gaccttcaca acttcgccgt ctttggtctg
      541 gtggaggaaa atattgtctg gtttgatgtc gcgatgaaca attccagccg cgtgggcttc
      601 ggccaggact tcacagatcg gaagcgcaat ttcaacacat cgctctgggg cgaggcggcc
      661 ctgttgggag atttctttcg ttaaggggtg gccactgagc agttccatca ccaggtaggc
      721 aatcccggcg gaactaatcc caaaatcgag gattgaaacc gcattcggat gctgaatccg
      781 acaggcagat acaccttcca accgaaaccg atccaagtcg ctttgagtgg cgtgaccgga
      841 acgtggttga aagactttga ccgccaccag ccggttcaac ccggtatgcg tggcttcgta
      901 cacggttcca aatccacccg ttccgatttt cttttccagc cggtacttcc cgtcgagcac
      961 ggttcccggc aaaacttccg acagggctga aaagatcatc tgtgcccgcc ggtttgattc
     1021 catcaggtct tcgcgagacc gggttaattc ttcattcttg cgggccagtt ctgcgttctt
     1081 ggtttcaaga tctttctgaa ggcgggaaat tttgagctgg ttttcaatgc gggccagcac
     1141 ttcctcaaac tggaacggtt tagtgacata atcaacgcca ccaacttcaa acgcccgcac
     1201 cttgtccatg acttcatcga gcgcgctgat aaagatgact ggaatatccc gactgcgcgg
     1261 gtcagctttg agttgctgac agacctcata gccatccatc tctggcatgg tgatatcgag
     1321 cattaccaga t
//
LOCUS       DAOQDO010002129         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123042_length_1331_cov_2.324451, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002129 DAOQDO010000000
VERSION     DAOQDO010002129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123042_length_1331_cov_2.324451"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..>1331
                     /locus_tag="PKZ53_25495"
     CDS             306..>1331
                     /locus_tag="PKZ53_25495"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNJ43861.1"
                     /translation="MKAILGNWNPSQSPEPISIWAKLRGRLGQTWNFEALSQNSEPAS
                     AQLAWQGQVTVQKSVPNIRFFIGASGLVSKADVVGEITARELRLERDSFGRVPLFWTA
                     HNGSIWFSTELSLLIEITGRSIVDSQGLYGYICFSYVPTPLTPIDGIAAVPAGETLCW
                     QAMTGTPESVEKTILRRSLRTHEWREDQVTVDDEDSAVSALRRLLTEATESLVTDVAG
                     NQVGVFLSGGLDSSIVAALLVRAGVKVKAYSLDFGGYGLPELEFAEAVASHLRIPLVK
                     VPAQPKHIRRALQSTVQALELPFGDGVTVPLYLLGQAAGKEVNIVFNGEGGDQLFISR
                     APMGGSDA"
BASE COUNT          324 a          323 c          331 g          353 t
ORIGIN      
        1 agaatgaaga atgaagaatg aagaatgaag aattgagaat gtagaaaatg agcggctgaa
       61 acctcttatt tttcaacttt caattcaccg gcttctgccc aaaactttcc tgcctcgtat
      121 tttacttagg aatggacaca caataatctg gtcaattgaa acaaacccta tcggaagtca
      181 aaaactcacc acagagacca cagaggaaca cagagggttc ggttgattga ttttcctctg
      241 tgctcctctg tgtcctctgt ggtgaaaaaa tgatgaagtt atttaatttt cattcctgag
      301 tctccatgaa agccattttg ggcaactgga atccctctca atctccagaa ccaatatcca
      361 tctgggccaa actgcgtggt cgcctcgggc aaacctggaa ttttgaagcc ctttcccaga
      421 actcagaacc cgcctctgct caacttgcct ggcaaggtca ggtcaccgtt caaaaatcgg
      481 tgccgaatat ccgctttttc atcggtgctt ccgggctggt atccaaagct gatgtggtcg
      541 gtgaaataac cgccagggaa ctgcggcttg aacgcgattc atttggccgg gtgccgcttt
      601 tttggaccgc acacaatggt tcaatctggt tttcaaccga actctccttg ctcatcgaaa
      661 taacgggccg ttcaattgtt gattcacaag ggttgtatgg ctatatctgc ttttcatacg
      721 tgcccacacc gttgacccca attgacggta tcgcggctgt acctgctggg gaaacactct
      781 gctggcaggc aatgactggt actccggaga gcgttgaaaa aacaattctt cggagatccc
      841 tgcgcaccca cgaatggcgt gaagatcaag tgacggttga tgatgaagat tcagccgttt
      901 cagcacttcg tcggctattg accgaagcca ctgaatccct ggtgactgat gttgccggca
      961 accaggtcgg ggtattttta tcgggaggac ttgattcatc cattgtcgcc gcgctcctgg
     1021 tccgggctgg ggtgaaagtc aaagcctatt cgcttgattt cggtggctat ggattgccag
     1081 aacttgaatt tgctgaagcc gttgccagtc atttgaggat tccgctggtg aaagttccag
     1141 cccagccaaa gcatatccgg cgggcactcc agtcaaccgt tcaagcgctt gagcttccat
     1201 ttggtgatgg ggtcaccgtg ccattgtatt tgctgggaca ggcggcagga aaagaggtaa
     1261 acattgtttt taatggagaa ggcggcgacc agctttttat ttctcgcgcc ccaatgggtg
     1321 gttctgatgc c
//
LOCUS       DAOQDO010002130         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123158_length_1330_cov_3.278431, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002130 DAOQDO010000000
VERSION     DAOQDO010002130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123158_length_1330_cov_3.278431"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..501
                     /locus_tag="PKZ53_25500"
     CDS             130..501
                     /locus_tag="PKZ53_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43862.1"
                     /translation="MMPKVTVGTGLALIAIGVVGFMNTGAKTALIPAVMGLVIGLAGL
                     IAMVKEDLRKHAMHVAVLIGLLGFLMTAKALIKLPVLLSGGTVQRPAAVIAQSVTAIL
                     LGIFVGLCVQSFVNARRQRTP"
     gene            830..>1330
                     /locus_tag="PKZ53_25505"
     CDS             830..>1330
                     /locus_tag="PKZ53_25505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43863.1"
                     /translation="MTQSAEKPTSLIGNFDPRIQVAGSGPVRLIPGQALEVNPDATLA
                     FPQPFSKAPAGKYLIQVWFDSATSPTTGLDQGDLVSAITPVDNLNPASTAPVELSLTD
                     RIGEGQRFEETDSIKLVEFQSPMLSAFWGHPMTIRAGIVLPPSYLKDPKRTYPTVYNV
                     HGFGGNH"
BASE COUNT          312 a          344 c          326 g          348 t
ORIGIN      
        1 tgcaaggttg gcttggacag gcgggctcag gtagctaaga tgcctgtttc gtcacatcct
       61 gggaaaatcc gaaccatcgg acaccctccc acttcattca caatcctttt tgttatgtgg
      121 taaatcgtta tgatgcctaa agttactgtt ggaactggac ttgctctaat tgctattggt
      181 gtggttggtt ttatgaatac cggggctaag acggcattga ttcctgctgt gatgggactc
      241 gtaattgggc tggctgggtt aattgcaatg gtcaaagaag atctgcgcaa acacgccatg
      301 catgtggctg tgctgattgg gttacttggg ttcctgatga ccgccaaagc cctcatcaaa
      361 ctgccggttc tgctttcagg tggaacagtc caacgaccgg cggcagtgat tgcgcagtca
      421 gtgactgcga ttctgctggg aatttttgtc ggtctgtgcg tgcaatcttt cgtcaatgcc
      481 cgccgtcagc gaactccata aacggcagat ttggctcgaa ctcacttgcc agctctggaa
      541 aaagaagatt tttctgaacc atcagacaca ggtgtggttt gaaggaacct gcaacctcaa
      601 cctcactcct caaaatccgg ttcgccactt caaaatctca aaatcttgag gcagctcaat
      661 gatgcgaaac ctcgtctttt cagttctcct ggtgtttcac ctgttttttg tgaacaccct
      721 tgtgacatct gcccaaacca cccctgtttc accaggaccg attcgatttc gaatcacagc
      781 aagggctgaa cttcttccca catcaacatc aggccggctg gttgtgttca tgacccaatc
      841 agccgaaaaa ccaaccagcc tgattgggaa ctttgatccg agaattcagg ttgccgggtc
      901 gggtccggtg cgactgatac ctggacaggc attggaagta aacccggatg cgaccctggc
      961 ctttccacag ccattttcga aagctccagc cgggaagtac ttgattcaag tttggtttga
     1021 cagcgcgact tcgcccacaa ccggtctcga tcaaggtgat ttggtaagcg ccattacgcc
     1081 cgttgacaat ctcaatcccg cgagcaccgc gccggttgaa ctgtcactca ccgaccggat
     1141 tggtgaaggt cagcgttttg aagaaacaga ttcgattaaa ctggttgagt ttcaaagccc
     1201 aatgttgtca gccttttggg ggcacccaat gacaattcgg gctgggattg tcttgccacc
     1261 cagttacctg aaggatccaa aacgaaccta tccaacggtc tataatgttc acggtttcgg
     1321 cggcaaccac
//
LOCUS       DAOQDO010002131         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123323_length_1329_cov_3.638148, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002131 DAOQDO010000000
VERSION     DAOQDO010002131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123323_length_1329_cov_3.638148"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKZ53_25510"
     CDS             <1..379
                     /locus_tag="PKZ53_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43864.1"
                     /translation="YVASGAGTIDLEGKSLLDAMNDGELDGMQHFDQVIERYIRQGII
                     SQEDGVAFSTNQSNLMLRLSGLGASDDFMGKGGSEIQRQQREREGQARPGMGGFNMPS
                     TGAPDRSSTRAIKTNSILDMIER"
     gene            492..1214
                     /locus_tag="PKZ53_25515"
     CDS             492..1214
                     /locus_tag="PKZ53_25515"
                     /inference="COORDINATES: protein motif:HMM:NF025094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNJ43865.1"
                     /translation="MQVTCTQCHTKFNLEEHNLPTQPFNVNCPNCRFTLMVTPPPKAD
                     PTLPASARPGPGPAGATDAPAGNDAVAQLVALLSSAMGLNASRAADGSAAPASWQRRH
                     AIICMAEAESIRKMQSQLDTAHFELTICETAARAVEIMRDIRVDIVILDPQFDANNQG
                     GITVLRHVSSMMPKYRRRIYLVLVSPQIKTLDTYMAFLNGVNLTVNSADLDTFREVLD
                     RSIRDFNELYRPYNAAAGLSPF"
BASE COUNT          307 a          401 c          322 g          299 t
ORIGIN      
        1 ctacgtggcg agcggcgccg ggacgatcga tctcgaaggc aagagcctgc tcgatgccat
       61 gaatgatggc gaactcgatg gaatgcagca ctttgatcag gtgatcgaac gctatatccg
      121 tcaaggcatt atcagccagg aagatggcgt cgccttctcg accaaccaga gtaacctgat
      181 gctccggctg tctggtctcg gggcgtcaga cgactttatg ggcaaaggcg gatctgaaat
      241 ccagcgtcag caacgcgaac gtgagggtca ggcccggcca ggaatgggag gcttcaatat
      301 gccttcgact ggtgcccctg atcgcagcag cacccgggcc atcaagacca attctattct
      361 ggatatgatc gaacgctaga aatccagggt tccgggttcc gggttccagg tcttcgaatt
      421 tatgtctctt cagtcctttt tgtcttttcc caaccccgaa cccctgaccc ctttttatta
      481 aaggatttgc catgcaagtc acctgcactc aatgccacac caagttcaac cttgaggaac
      541 ataacctccc aacgcagcca tttaacgtca attgcccaaa ctgccggttt acattgatgg
      601 ttaccccgcc tccgaaagcc gacccaacgc taccagcctc agcccgaccc ggccctggac
      661 cagccggggc aaccgacgca ccagcaggta acgatgccgt cgcccaatta gtggcgttac
      721 tctcttcggc catgggactc aatgccagtc gcgccgccga tggatcagcc gccccggcaa
      781 gctggcaacg tcgtcacgcc atcatttgta tggctgaagc tgaaagcatc cgcaaaatgc
      841 aaagtcagct tgatacggct cattttgaac tcaccatctg tgaaacagcc gcccgggctg
      901 tcgaaatcat gcgtgatatt cgggtggata tcgtcattct cgacccacag tttgacgcca
      961 ataaccaggg tgggatcacg gtgttacgtc acgtcagttc aatgatgccc aagtaccgcc
     1021 gccgaattta tctggttctg gtttcaccgc aaatcaaaac gcttgatacc tatatggcct
     1081 ttctcaacgg ggtgaacctg accgtcaatt ccgccgatct tgatacattc cgcgaagtcc
     1141 ttgaccgcag catccgggat ttcaacgaac tgtatcgccc ctacaacgcc gcagccggac
     1201 tttcaccttt ctaatcgtta tgtttgggct gaaggagtcg ggctgaagaa gccgggcgaa
     1261 agactttggt tttatttcat ccttcagccc ttagccccaa ccccttagcc ctcagcccca
     1321 aaccctcag
//
LOCUS       DAOQDO010002132         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123327_length_1329_cov_3.522763, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002132 DAOQDO010000000
VERSION     DAOQDO010002132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123327_length_1329_cov_3.522763"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKZ53_25520"
     CDS             <1..689
                     /locus_tag="PKZ53_25520"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNJ43866.1"
                     /translation="MMSGSFSTDFKVGGELKQNMMPNMSTVNGAGLVKIAQAALQGSK
                     LVSGITSLTKLSNSDQVSMKDVLMSATINNGRLSVKPFDVKMGNYATTISGSSGLDGT
                     LDYSMKMMVPAGQLGSQLQGFVNPGTTTNPNSEIPLNIGLGGTYKDPKPKLVSTEQKQ
                     QVKQAVTNVVEEKGKEKLKEVVKGTEAENVVNNLLGNKKDSVKKDSVKANPVQNVLQN
                     KLQNLLKKKK"
     gene            838..>1329
                     /locus_tag="PKZ53_25525"
     CDS             838..>1329
                     /locus_tag="PKZ53_25525"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNJ43867.1"
                     /translation="MNTPTILLLGGYGAVGRVVARLLLHETEADIIIAGRSKEKADAL
                     TAQLQAEFQGRVRAAFADAAKPETLKPILQDVQLLMVLSTTPEYSLDVAKEALAAGCD
                     YLDILVSESSFQNLQSIASEISQSGRTFIAQAGFHPGLPAPFIRLAAPYLDEYQSAVV
                     AMTM"
BASE COUNT          377 a          309 c          347 g          296 t
ORIGIN      
        1 gcatgatgtc cggttcgttc tcaactgatt ttaaggtcgg cggtgaactc aagcagaaca
       61 tgatgcctaa catgagcacc gtgaatggtg ctgggctggt taaaatagca caggcagcgc
      121 ttcagggcag taaacttgta tcagggataa cctccctcac caagttgagc aactcagacc
      181 aggtttcaat gaaagatgtg ctgatgtcgg caaccatcaa taacggacgg ctcagtgtaa
      241 aaccctttga cgtgaaaatg ggaaattacg ccaccactat cagcggaagc agcggactgg
      301 acggcacatt ggattattca atgaaaatga tggtgcctgc cggacagctg ggaagccaat
      361 tgcaggggtt tgttaaccca gggacaacga cgaatccaaa tagtgagatt cctctgaaca
      421 tcggactggg tggaacttat aaggatccca aaccaaagct ggtttcaact gagcaaaagc
      481 agcaggtcaa acaagcagtc accaatgtgg tggaggaaaa gggcaaagaa aagctcaaag
      541 aggtggtaaa gggtacggag gctgaaaatg tcgtaaacaa cctgctgggc aacaaaaagg
      601 acagtgtgaa gaaggatagc gtgaaagcga atcctgttca gaatgtattg cagaacaagc
      661 ttcaaaactt gctaaaaaag aaaaagtagc cctttcaacg tcatcaaaag cgcgctcttg
      721 gagcgcgctt ttttgtttgc cacaattggg caaaatatgc ctgacgtcat ttcccactga
      781 gtatggcggc agtatgtttg cagcaactcg tcactatcac acctgacggg ttcacatatg
      841 aacaccccca ccatcttgct tctcggcggc tatggcgctg tcgggcgcgt ggttgcacga
      901 ttactgctcc atgaaacgga ggctgacatt ataattgcgg gacgatccaa agaaaaggca
      961 gatgctttga ccgctcaatt gcaggcagag tttcaaggcc gcgtccgggc agcctttgca
     1021 gacgctgcca aaccagaaac cttgaaaccg atcttgcagg atgttcagtt gttgatggta
     1081 ctttcaacaa cgcctgagta ctcactcgat gttgcgaagg aggcactagc ggcaggctgt
     1141 gactatcttg acatactggt gagtgaatca tctttccaga accttcaatc gatcgcaagc
     1201 gagatcagtc aatcgggccg gacattcatt gcccaggctg gatttcatcc gggacttcca
     1261 gctcccttta ttcggctcgc agcaccgtac ctggatgaat atcagtcggc tgttgtggcc
     1321 atgacgatg
//
LOCUS       DAOQDO010002133         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123357_length_1329_cov_2.624804, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002133 DAOQDO010000000
VERSION     DAOQDO010002133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123357_length_1329_cov_2.624804"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..713)
                     /locus_tag="PKZ53_25530"
     CDS             complement(153..713)
                     /locus_tag="PKZ53_25530"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNJ43868.1"
                     /translation="MTSINYTFVTQSDNKLREAELILRRSLQRCDLDLPELQAVDVED
                     VVVHKALHAYQAIGRKPVLIEDTGLYFEAFNGLPGALVKWFLKRTETEGMCRMLDPFP
                     TRKAWAKTIVAVYDGQLSLFVGRVDGEIALHPRGDQGFGWDTIFIPDGETRTFGEMAP
                     EEKMQYSMRRRAFQAMAEFESIRREE"
     gene            complement(1078..>1329)
                     /locus_tag="PKZ53_25535"
     CDS             complement(1078..>1329)
                     /locus_tag="PKZ53_25535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43869.1"
                     /translation="EKYGELVTSLKEAGKLTSKMPRSRKNSHAFDDWHTTVLLVDWSS
                     GNRSINYGALDFYQGSSEVDQFIETFGRYTEHFQTTYAH"
BASE COUNT          341 a          348 c          298 g          342 t
ORIGIN      
        1 ctaagcacca agcaccaagc accaagcacc aagcactaag caccaagcac caagcactaa
       61 gcaccagtca ccaagcacca gtcaccaagc accagtcact aaaagactct tcattcttca
      121 ttcttcattc ttcattcttc attcttcatt cttcattctt cccggcgaat tgattcaaac
      181 tcggccatgg cctgaaaggc acggcggcgc atcgagtact gcattttttc ttccggagcc
      241 atttcgccaa aggtgcgggt ttctccgtcg ggaataaaaa ttgtgtccca gccaaagcct
      301 tgatcgcccc tgggatggag cgcaatttca ccatcaaccc gcccgacaaa caggctcaat
      361 tgcccgtcat acacagccac aatggtcttg gcccaggctt tccgggttgg gaacgggtcc
      421 agcatccggc acataccttc cgtttcagtc cgttttaaga accatttgac cagcgcgccg
      481 ggaagtccat tgaacgcttc gaaatacagc cccgtgtctt cgatcagaac gggtttgcgg
      541 ccaatagcct gataggcatg gagcgccttg tgaaccacaa cgtcttcgac gtctacggcc
      601 tggagttccg gcaagtccaa atcacaccgc tggagcgagc ggcgcaaaat gagttcagcc
      661 tcgcggagtt tgttatctga ttgggtgaca aaggtatagt tgatggatgt catatattac
      721 catttaggga tcaggattca gggttcaggg ttcaaggaaa tacagttttt ttcaatggtt
      781 tagccctccc tatgctgcga ggattttgtt cctaaatggt atgaaatcac ggagggcttt
      841 gttttgcgat tcaaaccact ggtttcaggc tgaacgatct ttgaaatcaa gcaaatcatt
      901 ttccggttat cggtgcaatc cctcattgtc gttgcgtgga ggtaatgtca caagaagcta
      961 caggcgctca agcttctcca atttggagtc agggtatcag gatcaggcat cagagaagca
     1021 taatcttttc gatgaattag ccttcccaca atacaacctc ttccttgcaa accggtttta
     1081 gtgggcatat gtcgtctgga aatgttcggt ataacgacca aaggtctcaa tgaactggtc
     1141 aacttcagaa gacccctggt agaaatctaa tgctccatag ttgatgcttc ggttaccaga
     1201 cgaccagtca accagcaaaa ctgttgtatg ccagtcatca aaagcatgcg aattctttcg
     1261 agaacggggc attttcgaag tcagcttccc ggcctctttg agtgaggtca ccaactcacc
     1321 atatttttc
//
LOCUS       DAOQDO010002134         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123570_length_1328_cov_2.413197, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002134 DAOQDO010000000
VERSION     DAOQDO010002134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123570_length_1328_cov_2.413197"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..>1328
                     /locus_tag="PKZ53_25540"
     CDS             252..>1328
                     /locus_tag="PKZ53_25540"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ43870.1"
                     /translation="MRNRVLVIGIDGTTFDWIDPLMQEGKLPNLARISQQGTRGPLET
                     VFPPITSTAWTSFATGKNPGKHGILEFIQRRGGSMREMAANATQRTGKALWEILGDAG
                     KKVIVTNFPVTYPPRPVNGVLISDFMTPRGRRDITYPNTLLADIEAKLGPYRLYLTQT
                     YAKGNVDGVLDEVFDEVDYKSKVNRYLMEREDWDFLVTHIWGTDRLQHETWHVMDQSH
                     PRHDSEEFKAHRARIVEFWQKVDQEVGNMVAAAGPETTVVLASDHGFGPAHKYCAFNI
                     WLMQEGFLKLKRTAQTRLKQAMFALGMTPEFAFKLSRQPIFKKIRPARGVGTQGNKVS
                     RLNQLFLSFNDVDWSRTIAFSKGNY"
BASE COUNT          347 a          351 c          310 g          320 t
ORIGIN      
        1 gatcaaaaat ccaaagcgca ctcatcccta cttttgggcc agttttattc aatccggcga
       61 atgggccaat ctggcgggaa aaaggtgacg tcaagctggt tttctggcaa gatgcccagc
      121 tacacaaatt ataaattatg agtctatttt cataccattt tggaatagag ccctcaaata
      181 ttgggaatca atgagtttaa atcattcttc attcttcatt cttcattctt cattctaaag
      241 aggtttctcc cttgcgtaat cgggttttag ttatcggtat tgacggaaca acatttgact
      301 ggattgatcc actgatgcag gaaggaaaac ttccaaacct ggcccggatc agtcaacagg
      361 gcacacgtgg cccactggaa acggtcttcc cgcctatcac ttcgaccgcc tggacgtcat
      421 ttgcgacggg taaaaatcca gggaaacacg gaattctgga atttatccag cgtcgaggcg
      481 gcagcatgcg tgaaatggcc gccaatgcca cccaacgcac cggcaaagca ctctgggaaa
      541 ttctgggcga cgctggaaaa aaggtgatcg tcaccaactt tcctgtcacg tatccaccgc
      601 gtccggtcaa tggcgttcta atttcagatt ttatgacgcc tcgcggacgc cgtgacatta
      661 cctatcccaa cacactgctg gctgacatcg aagccaaact tggtccgtac cggctctacc
      721 tcacgcaaac ctatgccaaa ggcaacgtag atggcgtgct tgatgaagtg tttgatgaag
      781 ttgactacaa atccaaggtc aaccgctatc tgatggaacg cgaagactgg gattttctcg
      841 tcacccacat ctggggaaca gatcgcctgc agcacgaaac gtggcacgtg atggatcagt
      901 cacacccacg ccacgacagc gaggaattca aagcccatcg cgcccgcatc gttgaattct
      961 ggcaaaaagt tgatcaggaa gtcggcaata tggtggcggc tgccggcccg gaaaccaccg
     1021 ttgttctggc ctctgatcac ggttttggac ccgcccacaa gtactgtgcc ttcaacatct
     1081 ggttgatgca ggaaggattt ctcaaactca agcgcacggc tcaaacccgc ttgaaacagg
     1141 ccatgtttgc gcttgggatg actccggaat ttgcctttaa actctctcgt cagccaattt
     1201 tcaagaaaat ccgtccagca cgcggcgttg gcacgcaagg caacaaagtc agccgtttga
     1261 accagttatt tctttccttt aatgatgtgg actggtcacg gaccatcgcc ttttcaaagg
     1321 gaaactac
//
LOCUS       DAOQDO010002135         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123790_length_1327_cov_2.405660, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002135 DAOQDO010000000
VERSION     DAOQDO010002135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123790_length_1327_cov_2.405660"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PKZ53_25545"
     CDS             complement(<1..1058)
                     /locus_tag="PKZ53_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43871.1"
                     /translation="MFVLHALWSSHNRLCLWGESSIKLSPGKTRKTQSRKTEVEPLHP
                     FAADIETLAQGITWVSLAEAAPQPGSKPASRKRISGNPYAHISGQLEVWLPTAGDRPL
                     PSPSFPVFDQVDLPQTPVGLNAWDIPVLTLDSTLAFELLFRLPGWPPPGYVFGSSVHY
                     FSKLAKFALELVTRGRVLPVIHQQAADYSSFWQPVLTAPEDAERMKILVDSFPALCRA
                     EVVSGQKGETPPSHLVRSALHHLVDAGVRMLLQEFSMDLPSNRQITEAVPAVVWLAGL
                     MNPHPTISTPPTSIHKLKQTVDAWHQSGTIGHKGGLTTCFRLVEPAAATAEPQKKAKN
                     QPVPPTPRAHPCRHPCGCR"
     gene            complement(1097..>1327)
                     /locus_tag="PKZ53_25550"
     CDS             complement(1097..>1327)
                     /locus_tag="PKZ53_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HNJ43872.1"
                     /translation="SASEELKKELREHVTKEIGALARPDDLRFTDSLPKTRSGKIMRR
                     LLREIASGSTTVGDTTTLEDMSVLERLREDEE"
BASE COUNT          291 a          333 c          400 g          303 t
ORIGIN      
        1 cgacatccgc atgggtgacg acacgggtga gctcgtggcg ttggtggaac gggctgattt
       61 ttggcctttt tctgaggttc agcggtggca gcggctggtt caaccagccg aaaacaggtg
      121 gtgagccccc ctttgtggcc gatggtccca gattgatgcc aggcatcaac cgtttgcttg
      181 agcttgtgaa tgcttgttgg tggggtggaa atcgttggat gcgggttcat caaaccagcc
      241 agccagacca ctgccggtac ggcttcggtt atctggcgat ttgatggtag atccattgaa
      301 aattcttgaa ggagcattcg aacaccggcg tccaccaggt gatgcagtgc tgatcgaacc
      361 agatgtgacg gaggggtttc acccttttgt cctgaaacaa cttcggcccg acacagggcc
      421 gggaaggaat ccaccagaat cttcattcgt tcggcatctt ctggtgcggt caaaaccggt
      481 tgccagaacg acgaatagtc ggctgcctgc tggtggatga cggggagcac ccgccctctg
      541 gtcacaagct ccagggcaaa ctttgccagc tttgaaaaat agtgaaccga acttccaaac
      601 acataccccg gcggtggcca gcccggcagc cgaaacagga gttcaaacgc cagtgttgag
      661 tccagggtca gaactggaat gtcccaggca ttgagtccaa ctggtgtttg aggcaggtca
      721 acctgatcaa aaactggaaa ggatggcgag ggcagggggc ggtcgccagc ggttgggagc
      781 catacttcca actggccgga aatatgagca tacgggttgc ctgatatccg tttacgggag
      841 gcgggttttg atccaggttg cggtgctgct tctgccagcg acacccaggt gatgccctgg
      901 gccagcgtct caatgtcagc cgcaaaggga tgaagtggct caacctcagt tttccgagat
      961 tgagtcttgc gggttttgcc tggggaaagt ttgatggatg actctcccca gagacaaagc
     1021 cgattgtggg aggaccagag ggcgtgaagg acaaacataa caaacctaaa gcggtgtcaa
     1081 gccaccgcac tccaaattat tcttcgtcct cgcgaagccg ttcaaggacc gacatgtctt
     1141 ccaaagtggt cgtgtcgccc actgttgtac tgccactggc aatttcacgc agcaggcgtc
     1201 gcatgatttt tccgcttcgg gttttgggaa gtgagtcggt aaaccgcagg tcatcaggtc
     1261 gagccagggc gccgatttct ttggtgacgt gttcgcggag ttcttttttc aattcttccg
     1321 aagccga
//
LOCUS       DAOQDO010002136         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123809_length_1327_cov_2.273585, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002136 DAOQDO010000000
VERSION     DAOQDO010002136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123809_length_1327_cov_2.273585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..>1327)
                     /locus_tag="PKZ53_25555"
     CDS             complement(188..>1327)
                     /locus_tag="PKZ53_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR family
                     protein"
                     /protein_id="HNJ43873.1"
                     /translation="DLDLSKYDQLASRFRQEQQESQNFLKSGIETRNASFNRMIEQIE
                     RVAIVSRAPILLMGPTGAGKSQLAKRIYELKKGRHLVSGKFVDVNCATLRGDTAMSTL
                     FGHVKGAFTGAMQNRPGLLRVADGGLLFLDEIGELGLDEQAMLLRALEEKVFMPLGAD
                     HEVRSDFLLIAGTNRDLTSQVHEGKFREDLLARINLWTFRLPGLKDRFEDIEPNLQYE
                     LEQFARLTGNRVTINKEARQRFLSFATSVEGRWAGNFRDLNAAITRMATLADGGRISL
                     AEVEEELGRLRESWRLPAEGNADSLVEKVLGPERSAALDRFDRVQLEDVLKVCREARS
                     ISEAGRELFAHSRLEKKNANDADRLRKYLAKFDLSWSDVSQTGVA"
BASE COUNT          311 a          383 c          307 g          326 t
ORIGIN      
        1 ttttaaccca atcgtgtcaa gccatacaat ctgccaatgg gctaacctta ctcaaactca
       61 aagcgtgaac ccatcagggg agggatcaaa atctctcgtc taaagacgat aggctgaacc
      121 tttttctgaa atgctctatt tgctaaccag cgattgtcat ttcaacgtag cattgtgatg
      181 tggatgatta tgcaactcct gtttgactga catcactcca ggaaagatcg aatttggcga
      241 ggtatttccg caatcggtca gcatcattgg cattcttttt ttcaaggcgc gaatgggcaa
      301 agagttcgcg gccagcttcg gaaatggatc gggcttcacg gcatactttg aggacatctt
      361 cgagctggac ccgatcaaac ctgtcaagcg cggcgcttcg ttccggtccg agcacttttt
      421 caaccagcga atcggcgttc ccttctgctg gcaatcgcca cgactcacgc aatcttccca
      481 gttcttcttc aacttcggcg agcgaaatcc ggcctccatc agccagtgtc gccatgcggg
      541 taatggcggc gtttaaatcg cgaaaattcc ccgcccaacg gccttccacc gaagtggcaa
      601 atgacaaaaa ccgctgccgg gcctctttgt tgatcgtcac ccggttacct gtaagccggg
      661 caaattgttc gagttcatat tgcagattcg gctcgatgtc ttcaaaccgg tctttgagtc
      721 ccggtagccg aaaggtccag agattgatgc gagccagtaa atcttcccgg aatttgcctt
      781 cgtgaacctg actggtcaaa tcgcggttgg tgccggcaat cagcaggaaa tcgctgcgga
      841 cttcgtgatc ggcacccagc ggcataaaca ccttttcttc cagtgcccgc aggagcattg
      901 cctgttcatc cagccccagt tcaccgattt catccagaaa caggagtcca ccatctgcca
      961 cacgcagcaa tccgggccgg ttttgcattg ctccggtaaa tgcgcctttc acatggccaa
     1021 acaacgtaga catcgccgta tcgccccgca gcgtcgcgca attgacatcc acaaacttcc
     1081 ccgaaaccag atgccgccct ttcttcaatt cataaatgcg tttcgcaagc tgcgactttc
     1141 ctgcccccgt cgggcccatc aacagaattg gcgcccgtga cacaattgcc acccgttcga
     1201 tttgctcgat catccggtta aagctggcat tacgggtttc aatgcctgac ttgagaaaat
     1261 tctgactttc ctgctgttcc tggcgaaagc gcgaagcaag ctggtcatac ttcgaaagat
     1321 cgagatc
//
LOCUS       DAOQDO010002137         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123818_length_1327_cov_2.151730, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002137 DAOQDO010000000
VERSION     DAOQDO010002137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123818_length_1327_cov_2.151730"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKZ53_25560"
     CDS             <1..285
                     /locus_tag="PKZ53_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNJ43874.1"
                     /translation="SGSYRTDGMVIIPCSMGSLGYIANGIARDLVHRAADVTLKEHRK
                     LILVPRESPYNAIHLENMLKLQRAGAMIVPASPGFYHRPTSIVELVDHFV"
     gene            385..1254
                     /locus_tag="PKZ53_25565"
     CDS             385..1254
                     /locus_tag="PKZ53_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43875.1"
                     /translation="MTDELVSNEDAIRETSSEVSAEILASKQKMLAQLDLLRRIQRAR
                     SAIFDDRRDLVFDVVEDVLQALLDNEETSEEELHILARRRDVSFEILRRMASDHRVAR
                     SHVLRRVLVLNPKLPASAGLRLVGQLYLFDLVFVLTTPALPMEVKTAAENLILQQYTG
                     IALGQKITLARRINGTRLIPHMLNDPSQEVVRAALDNPFLNENIVANAVRKANHSHVV
                     SLIADSPKWSVRKYVKQALLRSRYLSPGRATVLIQTLNALELRELKADSTVPVPIRTL
                     ISQISARLQSKRG"
BASE COUNT          300 a          337 c          364 g          326 t
ORIGIN      
        1 agtggttcct atcgtaccga cgggatggtg attattccct gcagtatggg gagcctgggc
       61 tatattgcta atgggattgc acgtgatctg gttcaccggg ctgccgatgt gacgctcaaa
      121 gagcaccgaa aactgatttt ggtcccgcgt gagtcacctt ataatgcgat tcacctggaa
      181 aatatgctca aactgcagcg agccggggcc atgattgttc cggcttcacc tggtttttac
      241 catcgcccaa catccattgt tgagttagtt gatcattttg tctaacgcat tttagaccat
      301 ctcgatattt cacatacggc ggatactatc tggcagggac gccgcacctg aggatatgac
      361 acattgagac gcgggtaggg aactatgact gatgaactgg tgtcaaatga agacgctatc
      421 cgggaaacga gttcggaagt ttcagccgaa attctggcca gcaagcagaa gatgctggcc
      481 cagttggatt tgctccggcg tattcaacga gcccgaagcg ccatctttga tgaccgccgg
      541 gacctggttt ttgatgtggt cgaagatgtg ctccaggccc tgctggataa cgaagaaacc
      601 agcgaggaag aattgcatat ccttgcccgg cggcgtgatg tgagttttga aatcctccgc
      661 cggatggcca gcgaccatcg tgttgccaga agccacgtgt tgcggcgcgt gctggtgctc
      721 aatccaaaat tgccggcctc cgccgggttg cgactggtgg gacagcttta tttgtttgat
      781 ctggtatttg ttctgacaac cccagccttg ccaatggaag tcaaaaccgc cgccgagaat
      841 ctgattttgc aacagtatac cggtattgcc ctggggcaaa aaatcaccct ggcccgacgc
      901 atcaacggga ctcgactaat tccacatatg ctcaatgatc ccagtcagga agtggtgcgg
      961 gccgccctcg ataacccgtt tttgaatgaa aacattgtgg cgaatgcggt gcgcaaggca
     1021 aaccatagcc atgtggtgtc gctcattgcc gatagtccga aatggtcggt tcgcaaatat
     1081 gtcaaacagg ctctgttgcg aagccggtat ttgtcaccag gacgcgccac ggtgttgatt
     1141 cagaccttga atgcgcttga actacgcgaa ttaaaggctg actcaaccgt tcctgtgccc
     1201 atccggacct taatctccca gatttcagcc cggttacaat caaaacgcgg gtagcgatct
     1261 tcagtctcag gaaatcgggt tccgggttcc gggttccggg ttccgggttc cgggttccgg
     1321 gttccgg
//
LOCUS       DAOQDO010002138         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_123855_length_1327_cov_1.914308, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002138 DAOQDO010000000
VERSION     DAOQDO010002138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123855_length_1327_cov_1.914308"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1327)
                     /locus_tag="PKZ53_25570"
     CDS             complement(<1..>1327)
                     /locus_tag="PKZ53_25570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ43876.1"
                     /translation="FLEAHSLGLDELKDPWGVPYRISVKTFREANNGSLVTNGPDKRP
                     GTPDDFTATEFDWPFFQATGKRIDRVVAQYHARTGGYIRDYETLKAELARENLALDKL
                     RDPWGRPYRFTFSIERENYVISVHSRGADGIETDPERYYGGDDFRVWTSRCNYFVDTW
                     AILEQAVGDVLPEIILASDKDQAFRAKLAAAGIVFDELRDVWESPLYLNFRHLELKPI
                     NDNKSGMSQMQVVYRYGTVSICSRGPNQVQGDADDFVLGILATRTATSQEGQSPDEKA
                     PSENDTGTTGTLMGTVMDESGAVITDVKIEAIAKGTGQTFSTKTNENGQYFLVQLPPG
                     WYEVNANAGGFQYVRYENVLLRGNRMTVANVTLVVGGSSELIIVSSGEVLLQTTNSEV
                     RVRTREVQPVASSVPVPLFTPRVRRYFPETLLWQPQVETDQSGRARLNFK"
BASE COUNT          313 a          359 c          320 g          335 t
ORIGIN      
        1 gtttgaaatt gagccgggcg cggccacttt ggtcggtttc cacctgtggt tgccagagca
       61 gagtttccgg aaaatatcgt cgcactctgg gggtaaagag cgggacggga acactggagg
      121 caaccggttg cacttcccgt gtcctgactc gcacttctga gttggtggtt tgtaacaaca
      181 cttcacctga cgaaacaatg atgagttctg aggaaccacc aaccaccagc gtgacattgg
      241 cgacggtcat tcggttgcca cgcagcaaaa cattttcata tcgaacgtat tgaaacccgc
      301 cagcattcgc attgacctcg taccatccgg gcggcaattg caccagaaaa tactggccat
      361 tctcatttgt ttttgtggag aaggtctggc ctgtcccttt ggcaattgcc tcaattttga
      421 catcggtaat cacagcgccg ctttcatcca tcacggttcc catcagagtc ccggttgtgc
      481 cggtatcatt ctctgaagga gctttttcat caggagattg accttcctga ctggttgccg
      541 ttcgggtcgc cagtattccc aggacaaaat catccgcatc gccctgaacc tgatttggtc
      601 cacggctgca gattgaaacc gtgccatagc gatacacaac ctgcatctga gacattccag
      661 atttattgtc atttatcggc ttgagttcca ggtgtcggaa attgagatag agcgggcttt
      721 cccacacatc acgcagttcg tcaaagacga tcccagccgc tgccagtttc gctcgaaaag
      781 cctggtcttt gtcactggcg agaataattt ccggcaacac atcccccaca gcctgctcaa
      841 gtattgccca ggtgtccaca aaataattgc accgggaagt ccagactcga aaatcatcgc
      901 caccatagta tcgttctggg tcagtttcaa ttccatctgc gccacggctg tgcactgaaa
      961 tgacatagtt ttcccgttca attgaaaaag taaagcgata gggccttccc cacgggtcgc
     1021 gtagtttatc aagcgccagg ttttcgcggg caagttccgc tttcaaggtt tcataatccc
     1081 ggatgtagcc tcccgtgcgg gcgtggtact gcgctacaac ccgatcaatc cgtttgccgg
     1141 tggcctggaa aaaaggccag tcaaattcgg tagcagtgaa atcatccggc gtgccgggac
     1201 gtttatccgg cccatttgtt accagtgaac cgttatttgc ctcacgaaat gttttgaccg
     1261 aaatgcgata tggaacaccc cagggatctt tcaattcatc gagccccagt gaatgtgctt
     1321 ccaaaaa
//
LOCUS       DAOQDO010002139         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124120_length_1325_cov_3.288976, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002139 DAOQDO010000000
VERSION     DAOQDO010002139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124120_length_1325_cov_3.288976"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PKZ53_25575"
     CDS             <1..1252
                     /locus_tag="PKZ53_25575"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43877.1"
                     /translation="RPVTVEAVQQLIPADAALVEFAVYRLYDAKTQTYGATHYGVYVL
                     TSQGVIKSADLGETEPIDGTIAQLRQALRTSKMTPGERRNLVKMTGTPQNSETVMVKA
                     RVVDQLVMAPVRKLIGDQTRLLISPDGDLNLIPFAALVTDQGKFLVEDYLMTYLTSGR
                     DLLRLQTKLPSQQPPLVVANPDYAAGSGPIVAGQSVNPLSPLPGTGQEGLAIQKLFAD
                     ARLVLQADATKDVVKNVHRPELLHIATHGYFLKDIQKTISGEDERGIEQNRSLVLGNL
                     SLENPLLRSCLFFAGANRSGETNNGILTALEVAQLDLWGTKLVVLSACDTGVGEVKTG
                     DGVYGMRRALVLSGSAAQMISLWPVSDLGTRELMAEYYKRLKAGEGRSEALRNTQLKM
                     LKDPKRRHPFYWASFIQSGEWKKL"
BASE COUNT          322 a          327 c          376 g          300 t
ORIGIN      
        1 cagacccgtg acggttgaag ccgtgcaaca actcattcca gccgatgcgg cactggtgga
       61 atttgccgtc tatcgtctgt atgacgccaa aacccaaacc tatggcgcaa cgcattacgg
      121 tgtctatgtg ttaaccagcc agggagtgat caagagtgcc gatttagggg aaactgaacc
      181 aatagatggc acgattgctc aattgcggca ggcgttgcgt acttcaaaaa tgacgcctgg
      241 cgagcggcgg aatttggtca aaatgactgg cactcctcag aattcagaga cggtgatggt
      301 caaggcccga gtcgttgacc agttggtgat ggcaccggtt cgcaagctga ttggcgacca
      361 aacccggttg ctgatttcac cagatggaga cctgaatctg attccatttg ctgcgctggt
      421 gaccgaccag gggaaatttc tggtcgaaga ctacttgatg acctatctaa ccagcggacg
      481 cgatttgctg cgattgcaga caaaactgcc ttcccagcaa cccccgctgg tcgtggccaa
      541 cccggattat gccgccggaa gcggaccaat cgtggctggt cagtcggtca atcccttatc
      601 gccacttcct gggactggcc aggaaggtct ggcgatccag aaattatttg cggatgcccg
      661 gctggtgctc caggctgatg ccaccaaaga tgtcgtcaaa aatgtgcatc gtcccgaact
      721 gttgcatatc gctacccatg gctattttct caaggatatt caaaaaacaa tctccggcga
      781 ggatgaacgg ggtatcgaac aaaatcgctc gctcgtcctt ggaaacctga gtcttgagaa
      841 cccactgctc cggtcgtgtt tgttttttgc gggtgccaat cgtagcgggg aaaccaacaa
      901 tggcattttg acagctctgg aagtggcgca gcttgattta tggggcacca aactggtggt
      961 gctttcagcc tgcgacaccg gtgtgggcga agtcaaaacc ggtgatggcg tctatggaat
     1021 gcgccgggcc ctggtgctgt ctggaagtgc cgcgcaaatg atcagtctct ggccagtctc
     1081 tgatcttgga acccgagaat tgatggctga atattacaaa cggttgaagg ccggggaagg
     1141 ccgttccgaa gcgctgcgca acacccaact caagatgctc aaagatccga aacggcggca
     1201 tccattttac tgggcaagct ttattcaatc gggtgaatgg aaaaaacttt aggactgaag
     1261 aaagtgggct gaagaaattg gactgagcgt caggataagg attttaggcc cgtaggggcg
     1321 ttgtc
//
LOCUS       DAOQDO010002140         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124126_length_1325_cov_3.091339, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002140 DAOQDO010000000
VERSION     DAOQDO010002140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124126_length_1325_cov_3.091339"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PKZ53_25580"
     CDS             <1..1180
                     /locus_tag="PKZ53_25580"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ43878.1"
                     /translation="HKDHLDKFYAIFSDVLLHPRFDQAEFDRVREDAVNYLKTSLRST
                     NDEEFGKQTLETEIFRGHAYDHVIGGSVRSLNALKLEDVKAFYQQYYTQSNLMIGVAG
                     DVPKTFVAQMEKDFSALPVGKPAELRAGTITPPSGLEVTLIEKDTDATAISLGFPVNV
                     TRKDSEFYALLVANSYLGEHRTFNGVLMQNMRGKRGLNYGDYSYVEHFVQEGGSTFAL
                     PNVPRQKQYFSIWIRPVPNAARLFALREAMWETDRLVKNGMTQEQFESTRKFLLNYSR
                     LWVQTLSRRLGYRMDSKFYGIEDYIQEIQTRLPKLTLAEVNAAIKKHLTSQSFKVVMI
                     TRDAGSLKQQMLANTVSPMKYDSATPDDILKEDKEIEKFPLAATEKKIRIVKAEEMFE
                     "
BASE COUNT          350 a          325 c          357 g          293 t
ORIGIN      
        1 ccacaaagat cacctcgaca agttttatgc gattttctct gacgtgttgc tccatccccg
       61 gtttgatcag gctgaatttg atcgggtccg cgaagatgcg gtcaattacc tgaagacctc
      121 gctccggagc accaatgacg aggaatttgg caaacagacg cttgaaaccg aaattttccg
      181 gggccacgcc tatgatcacg tgattggtgg ctcggtgcgt tcgctcaacg cgttgaaact
      241 ggaggatgta aaagcgtttt accagcagta ttacacccaa tccaacctga tgattggtgt
      301 tgccggagat gttcccaaga cgtttgttgc ccagatggag aaagattttt cagcccttcc
      361 agtgggaaaa ccggcggaac tccgggctgg aaccatcacg cctccgtccg ggctggaagt
      421 cacgttgatt gaaaaagata ccgacgccac agcgatttcg ctcggctttc cggtcaatgt
      481 gacgcgcaaa gattcagagt tttacgcctt gctggtggcc aattcctatc ttggcgaaca
      541 ccggaccttt aacggtgtgc tgatgcagaa tatgcgcggc aagcgtgggt taaactatgg
      601 cgattattct tacgtcgagc actttgtcca ggaaggcggc tcgacgtttg cgctgcctaa
      661 cgtcccccgt cagaaacagt atttcagcat ctggattcgg ccagtgccca atgccgcccg
      721 gttgtttgcg ctccgggagg caatgtggga aaccgaccgg ctggtgaaaa acgggatgac
      781 ccaggagcaa tttgagtcaa cccgcaagtt cctgctcaat tacagccggt tgtgggttca
      841 gacgctgagc cgccgtctgg gatatcgaat ggattcaaag ttttacggca ttgaagatta
      901 cattcaggaa atccagacgc ggttaccaaa actcaccctg gccgaagtca atgccgcaat
      961 caaaaaacac ctcacttcac agtcattcaa agtggtgatg atcacccgtg atgccgggtc
     1021 gctcaaacag cagatgctcg ccaacacggt gtcacccatg aaatatgaca gcgccactcc
     1081 ggatgatatt ctcaaggaag acaaggaaat tgaaaaattc ccgctggctg ccactgagaa
     1141 gaaaattcgg attgtgaagg ctgaggaaat gtttgaataa gtgagggttc agggttccgg
     1201 gtttgggaag acaaaagtga gggttcaggg ttcagggttc agggttctgg aagacaaaaa
     1261 ggacgaaaag gacgaaaagg acataaattc gaaaacccgg aacccggaac ccggaacccg
     1321 gaacc
//
LOCUS       DAOQDO010002141         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124159_length_1325_cov_2.485039, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002141 DAOQDO010000000
VERSION     DAOQDO010002141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124159_length_1325_cov_2.485039"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..>1325)
                     /locus_tag="PKZ53_25585"
     CDS             complement(144..>1325)
                     /locus_tag="PKZ53_25585"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43879.1"
                     /translation="DTRPPVTDDSSPVNFSDFLAQLGKTPQRATGSAQPSPELEDRQP
                     RTPQTEPLLATLSPTGPAPSPATSDRNQNSAVSKELSRIKAELREMKYAFQLLSGSML
                     RQRLEAEMAAEFEVCPELYDSPYFEAFLDLAALGLNPNQARYAVQSMLNRRVITTRPR
                     TREDVISMGLTSLLIDKVPFGKNILETVPGVSAPAGLIFVGPTGVGKTTTIAKLAAQA
                     TLHTKHRVELISLDTYRIGAVEQIKTYAEIIGVRCHIARSVTELDALVHKHANRSLVL
                     IDTVGRSPNDLSDHFELAEYLRQSKSLLKTLVLQTTTNPVDAQIAFRKFSVYGLDQLV
                     MTKWDETSRPGGALEIIEETKLPLAYLGTGQRIPEDLVPATPNAVAVRILTSPASAAA
                     A"
BASE COUNT          293 a          333 c          391 g          308 t
ORIGIN      
        1 ggcgggcaga tcggttgtaa accgaaacac accgccccgt gcgggatgga attggcatag
       61 gtgacacaac tcattctcct ggttactgga tgtgcaccac accctgaatt ggggccacac
      121 accacactga cgttccctca ggtttaagca gcggcggctg atgcgggaga tgtcagaatt
      181 ctgacggcga cggcatttgg cgtggccgga accaggtctt cagggatccg ttgccccgtt
      241 ccgaggtagg ccagcggaag tttggtctct tcaatgattt caagtgctcc gcctggtcga
      301 gaggtctcgt cccatttggt catcaccagt tgatccaacc cgtacactga aaatttacga
      361 aaggcaatct gggcatcaac cgggttggtt gtggtttgta ataccagtgt tttcagtaat
      421 gattttgact ggcgaagata ctcagccagt tcgaaatgat cggaaaggtc attggggctg
      481 cggccaactg tgtcaatcag gaccagtgac cggttggcgt gtttgtggac cagcgcatcc
      541 agttcggtga cggagcgggc aatgtgacac cgaaccccaa taatttcagc ataggtcttg
      601 atttgttcga ccgcgccgat ccgatacgta tcgagcgaaa taagttcgac ccggtgtttg
      661 gtatggagtg tggcctgagc cgccagtttg gcaatagtgg ttgtctttcc gacccccgtt
      721 gggccgacaa aaatgagtcc agcgggtgct gatacacccg gaacggtttc cagaatgttt
      781 tttccaaacg gcactttatc aatcagcagc gaggtcaatc ccatagaaat cacatcttct
      841 ctggttcggg gtcgagtggt aatcacacgc cggttgagca tggactgcac tgcgtaccgg
      901 gcctggtttg gattcaggcc aagtgccgcc agatcaagaa aggcttcaaa gtacggtgag
      961 tcatacaact cagggcacac ttcaaattca gccgccatct cggcctccag tcgctgccgc
     1021 aacatcgaac cgctcaaaag ctgaaaggca tatttcattt cccggagttc ggccttgatg
     1081 cgtgagagtt ctttggaaac agccgaattt tgatttcgat ctgaggtggc cggtgatggc
     1141 gccggccccg tcggagacag ggtagccaaa aggggttcgg tttgcggggt gcgcggctgc
     1201 cggtcttcca gctctggcga cggttgagcc gaaccggtcg cacgttgagg tgttttgcca
     1261 agctgggcca aaaaatctga gaaattaacc ggggacgaat cgtcggtcac aggggggcgg
     1321 gtgtc
//
LOCUS       DAOQDO010002142         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124213_length_1325_cov_2.038583, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002142 DAOQDO010000000
VERSION     DAOQDO010002142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124213_length_1325_cov_2.038583"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKZ53_25590"
     CDS             <1..387
                     /locus_tag="PKZ53_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43880.1"
                     /translation="PSGESQNGYLRLTDVYNLQLQSDLVVLSACQTGLGKEIKGEGII
                     GLTRGFMYAGAPRVVSSLWMVDDSATAELMKRFYAAILGPKRLSPAAALRQAQLELAG
                     KGTRRFPYYWAAFQLQGEWKPMPAHR"
     regulatory      471..539
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            561..1040
                     /gene="crcB"
                     /locus_tag="PKZ53_25595"
     CDS             561..1040
                     /gene="crcB"
                     /locus_tag="PKZ53_25595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNJ43881.1"
                     /translation="METLWKYLAIALGGAIGSMVRYYLGTTALSRWGAPFPTATFVIN
                     ITGSFILGFFLTLIGDRFPVHPNFRLAVAVGFVGAYTTFSTFEFEAATLVAVRDVMRA
                     AVYVALSVAVGFAAVWFGMYLAKRVAGAFPARVITVSSAPSHLVTSPALTTLQPEEK"
     gene            1053..>1325
                     /locus_tag="PKZ53_25600"
     CDS             1053..>1325
                     /locus_tag="PKZ53_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43882.1"
                     /translation="MGQLSIRWLIGTSLGLVFLASMSFPSPAREPLVQKDPIDIDPLP
                     FYTAVKVLGAHKKNPDTEQLASFATTLHIERGFNYTFYLCADGTSSA"
BASE COUNT          261 a          342 c          374 g          348 t
ORIGIN      
        1 cccagcggcg aatcgcaaaa tggctatctc cggctgaccg atgtctacaa cctccagctt
       61 caatcagatc tggtggtgct ctcggcctgc cagaccgggt tgggcaaaga aattaaaggt
      121 gaagggatca ttggcctgac ccgagggttt atgtatgccg gtgctccacg ggtggtttca
      181 tcgctctgga tggtagatga cagtgccacg gctgaattga tgaagcggtt ttatgccgca
      241 atcctcggcc ccaagcgcct ttcaccggcg gcggcgcttc gccaggccca gcttgaactg
      301 gctggaaaag gcacgcggcg gtttccctac tactgggcag cgtttcaatt gcaaggtgaa
      361 tggaaaccca tgccggctca ccgataggca ctttggcgac tgaaccagtt gcatcgggca
      421 atcgcttcac agtatggttg aggcgacgtt ccatcttcca aagtgaacaa tttttcgatg
      481 gcgatggggt tcgccgataa ccactctgtc tttccagagt tgataactcc tgctacgttc
      541 gacagttgag gagacctggt atggaaacac tctggaaata tctggcgatt gctctgggtg
      601 gagcgattgg gtcaatggtt cgctattatc ttggcacgac ggcactctca cgttggggcg
      661 ccccgtttcc aaccgcgacc tttgtgatta acatcaccgg atcgtttatt ttgggctttt
      721 ttttgacctt gattggtgac cggtttccgg tgcatcccaa cttccggctg gcggttgccg
      781 ttggatttgt cggcgcctac accacgtttt caacctttga atttgaagcc gccacgctgg
      841 tcgctgtccg ggatgtcatg cgggctgctg tttatgtcgc cctcagcgta gccgttggtt
      901 ttgccgctgt gtggtttggg atgtacctgg caaaaagagt agctggcgcc ttcccagccc
      961 gtgtgattac tgtgtccagt gccccctcac atctggtgac gtcgccagca ctgaccactt
     1021 tgcaacctga ggaaaaatag tgtggagggc ccatgggtca actttcaatt cgctggctga
     1081 ttgggacgag tttgggactg gtgtttctgg cttcgatgag tttcccgagc ccagccaggg
     1141 agccgctggt tcaaaaagat ccgattgaca tagatccgtt gccgttttat acggcggtga
     1201 aagtccttgg ggcacataag aaaaacccgg atactgagca actggcttcg tttgccacga
     1261 ccctgcatat tgaacgcggg tttaattaca cgttttatct ctgcgcggat ggaacatcgt
     1321 ctgct
//
LOCUS       DAOQDO010002143         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124222_length_1325_cov_1.945669, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002143 DAOQDO010000000
VERSION     DAOQDO010002143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124222_length_1325_cov_1.945669"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..>1325
                     /locus_tag="PKZ53_25605"
     CDS             291..>1325
                     /locus_tag="PKZ53_25605"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ43883.1"
                     /translation="MYPQVTLMIEENGTQRLVTRSFSVLRVGRTAENDLVLADPGVGR
                     AHLQFQWNNQGLQVVDGGSANGTFVNDTRVVGAITIHPDDVVRVGTARCLIQVRFAPP
                     HLEPQPVAVPVQPAVLIPTGYRAGRNHPNSSPNPSRVPIWAGGVAGFLVLAIIGLLVY
                     QSKSNPTPGAVEPNPPLPVVSVSEGTPTDFPRVDSMNLPEVSPPPVSTSLSLEQALED
                     FVSRITSDSRSYVLPTQAVDCMADVSAAITRLRNSTTLAKTLCELNQSSSLASQIRKR
                     SLLKPVFVVCAALAVSNGGTSGSDVAEMCVGPLLTSLDSLGVKFGISSGEDVLFVVAA
                     YAYGQGTEKFH"
BASE COUNT          306 a          357 c          345 g          317 t
ORIGIN      
        1 gggattttgg gctggatatg caaaagctct cttcctacac catcgaaagc cagtcagatg
       61 aaacatttga tcaaaccgaa gaaaccggag cgctatagtc tttcagaaag agaaaccacg
      121 caatacatgg aacacacaga aaataaatcc aaattcttca atgatttctg agacttgcca
      181 aagtaaaaat tcaagaaata tttattctga aaagctctag caaccagcaa accagttgaa
      241 gaaattgtat ttccctaaac cctgatccct gacccctgac ccctgataat atgtatccac
      301 aagtcaccct gatgatcgaa gaaaacggca cccagcgact cgtgacccgg tcgttttctg
      361 tgttgcgggt gggccgcacg gctgagaatg acctggtgct ggcagatcca ggggttggcc
      421 gcgcccatct gcagttccaa tggaacaatc aggggctcca ggtggttgat ggaggctcgg
      481 ccaatgggac cttcgtcaat gacacacggg tcgtgggtgc catcaccatc cacccagatg
      541 atgtggttcg ggtcgggact gcccggtgtt tgattcaggt tcgatttgcc cctccgcatt
      601 tagaaccgca accggttgca gtacctgttc agccagccgt tctcatccct accgggtacc
      661 gggcgggccg gaaccacccc aactcgtcgc ccaatccgtc gcgggtacca atttgggctg
      721 gaggagtggc tgggtttctg gtactggcca taatcggtct gctggtatac cagtcgaagt
      781 ccaatccaac gccaggtgcg gtcgaaccga atccaccttt gcctgtggtg agcgtttcgg
      841 aaggtacccc gactgacttc cctcgggtgg acagtatgaa tctacccgag gtctccccgc
      901 cgccagtgtc cacttcgctc tcgctcgaac aggcacttga agattttgtc agtcgaatta
      961 cctctgattc ccgatcctat gtcttaccaa ctcaggcggt tgactgtatg gctgatgttt
     1021 ccgcggcgat tacccgctta cgaaactcaa ccacgctggc caaaaccctg tgtgaattaa
     1081 accagtcttc ctcgctcgca agtcagattc gaaaacgcag cctgttgaaa ccggtttttg
     1141 tcgtgtgtgc ggcactggcg gtgtccaacg gcggcacgtc agggtcagac gtggctgaga
     1201 tgtgtgtggg ccccttgctg acgtcacttg attcactggg ggtgaaattt ggtatttcca
     1261 gcggtgaaga cgtcttgttt gtggttgccg cctatgccta tggtcagggg acagagaaat
     1321 tccat
//
LOCUS       DAOQDO010002144         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124376_length_1324_cov_2.297872, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002144 DAOQDO010000000
VERSION     DAOQDO010002144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124376_length_1324_cov_2.297872"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1206
                     /locus_tag="PKZ53_25610"
     CDS             388..1206
                     /locus_tag="PKZ53_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HNJ43884.1"
                     /translation="MDTQIIATYCLCDDVLKGFGHWEDPQSQMTDAEVLTSGVVAALH
                     FGGNLSRACQMLGEQGYIPKMLGKSRFNRRLHRVAEQVGVLFGVLGELWKAWNTDSTY
                     VLDSFPLLVCENCRIRRCHLCQGEEWRGYQASKKRYFYGVRLHFLVTGQGEPVEFFLT
                     PGSVNDTTALKMYHLDLPEGSKVIGDKAFNDYRYEDLLKEANMTLLPLRKINSKRPYP
                     PWQAYWLGVQRKVVETAGSLIERLLPKHIHAVTFRGFELKVALFVLASSISLLC"
BASE COUNT          346 a          289 c          344 g          345 t
ORIGIN      
        1 aagtatttcg acacggtcaa gattgcgctg ccgcgcctga agcacctgct gcgccagcag
       61 tttgttgcaa ttgctgacat ccagttacta acatacccgc cactagcaga gttgcaagtg
      121 taatgtggga tttgagtttc atcgtcttga tctctttcgt ttgttgttta aggaaaagat
      181 tcattcacag gtttaagcaa agaccatcat agactgaggc ttcaccacaa ccccggataa
      241 atttaagacg aatatacaca aaaagtgagg ttatgtaacc attgattcag ggttacttaa
      301 tttaacccaa gttgctaccc acgatgaaag tccatgagga atccccaaag tgtagttgaa
      361 agcattgcac taaaggagat gaacctcatg gacacccaaa tcatagcaac gtattgcctg
      421 tgtgacgatg tactgaaagg ctttgggcat tgggaagatc ctcagagcca aatgaccgat
      481 gcagaggtat taaccagcgg ggtggtggca gctctgcact ttggcggcaa cctgtcacgg
      541 gcctgccaaa tgttgggtga acaaggttac atcccgaaga tgctgggcaa aagccgcttt
      601 aaccgccgtc tgcatcgggt ggcggagcag gtgggggtac tgtttggcgt gttgggtgaa
      661 ctctggaaag cgtggaatac agacagcacc tacgtcctgg acagtttccc gctcctggtg
      721 tgtgaaaatt gccggattcg ccgatgccac ctgtgccagg gcgaggaatg gcggggttat
      781 caggccagca agaaacggta tttttacggt gtcaggctgc attttctggt gactgggcaa
      841 ggggaaccgg tcgagttttt cctgacaccg ggttccgtga acgatacgac agccctgaaa
      901 atgtaccacc ttgacttgcc tgaaggctca aaggtcatcg gggacaaggc gttcaatgat
      961 tatcggtacg aagatttgct caaggaagcc aacatgacct tgttgcctct gcgaaaaatc
     1021 aactccaaac ggccttaccc accttggcag gcttattggt tgggcgtcca gcgcaaagtg
     1081 gtggaaactg cgggaagcct gattgaacgg ctgttgccaa aacatattca tgccgttact
     1141 tttcggggat ttgaactcaa agtggcactg tttgtgctgg catccagcat cagtttgctc
     1201 tgctgaaggt ggcaacttgg gttattatta atctataact atttttgttg gttaaacaaa
     1261 aattattcaa ttttttaaaa tgatggttat ctagcaaata tattgactca cctatacgtg
     1321 gtgt
//
LOCUS       DAOQDO010002145         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124527_length_1323_cov_2.781546, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002145 DAOQDO010000000
VERSION     DAOQDO010002145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124527_length_1323_cov_2.781546"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKZ53_25615"
     CDS             <1..614
                     /locus_tag="PKZ53_25615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43885.1"
                     /translation="QSFFDTSKRQLFLLVCLSTLVLLFVKKSFIENETAAFEFLEEQS
                     AGSVLYVRSALQYFSIPLIYAWKFIVLAFVIWTGCFTFGYRVTYTQCWKVVMTSELIF
                     IIPELLRIIWFLVVDTDPNIYEIRAFYPFSLMNFFAFEDVPAQWHYPLKSLNIFEVGY
                     WLLAAKGVQHFSRRSYREGFLTILFTYVPIYILWLAFFVVVYK"
     gene            677..>1323
                     /locus_tag="PKZ53_25620"
     CDS             677..>1323
                     /locus_tag="PKZ53_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNJ43886.1"
                     /translation="MNFQKINNLTGWIVFGVALVTYWLTFEETASYWDCGEFIAVSYK
                     LEVPHPPGAPLFLLLGRVFSFLSFGDVTKVAYWINFLSVLASAFTILFLFWSITLFGR
                     KMMGITKSEELTTDKTWVLMGAGLVGSLAYTFSDSFWFSAVEAEVYAMSSFFTAFVVW
                     GVLKWDVINDESKANRWLILIAYMVGLSIGVHMLNLITIPALGLIYYFKKYKPTF"
BASE COUNT          268 a          353 c          300 g          402 t
ORIGIN      
        1 cccagtcttt ttttgacacc tccaagcggc agctgttttt gctcgtgtgc ctaagtacgc
       61 tcgtcttgct gttcgtcaag aagtccttca tagaaaacga aactgcggcg tttgagttcc
      121 ttgaagaaca gtcagccggc tcagtattat acgtccggag cgcacttcag tacttttcca
      181 taccccttat ttacgcctgg aagttcatcg ttctggcgtt tgtgatctgg acgggctgct
      241 tcactttcgg ctatcgcgta acctacacac aatgctggaa agttgtgatg accagtgaac
      301 tgatctttat cattcccgaa ctgcttcgca tcatttggtt tttggtggtg gacacagacc
      361 ccaatattta tgaaatccgc gccttctacc ccttttccct gatgaacttc ttcgccttcg
      421 aggatgtgcc ggcccaatgg cattatccac tcaagtccct caacatcttt gaagtcggat
      481 actggctatt ggcagcgaag ggtgtccagc acttttcccg caggagctac cgcgaaggat
      541 ttctgaccat tttgtttacc tatgtgccga tttatatact ctggctggct tttttcgttg
      601 tggtctacaa ataggccgcc cgccgatcaa ccaatctttt tagctaattt gcccgttcat
      661 tcttgactgt aacaccatga attttcaaaa gatcaacaac ctcaccggct ggatcgtttt
      721 tggcgtcgcc ctggtcacct actggctgac gtttgaagaa accgccagct attgggattg
      781 tggtgaattt atagcggttt cttataagct ggaggtaccc caccctcccg gggctcctct
      841 ctttttgttg ctgggtcgtg tgttttcctt tctgtctttt ggggacgtca ccaaagtggc
      901 ctactggatc aacttcctga gtgtgctggc cagcgccttc accatcctgt tcctgttctg
      961 gtccatcacc ctttttggcc gcaaaatgat gggcatcaca aaatctgaag aactcaccac
     1021 cgacaagacc tgggtgctca tgggcgcagg tctggttggc tctctcgcct ataccttttc
     1081 agattcattc tggttttcag ctgtggaagc cgaagtatac gccatgtcgt cgttttttac
     1141 agcgttcgtg gtgtggggcg tactgaaatg ggatgtgatc aacgatgaaa gtaaggccaa
     1201 tcgctggctc attctcatcg cctatatggt cggcctgtct atcggggtgc acatgctcaa
     1261 cctgatcacc attccggccc tcggcctgat ctactatttc aaaaaataca agcccacctt
     1321 ctg
//
LOCUS       DAOQDO010002146         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124580_length_1323_cov_2.115931, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002146 DAOQDO010000000
VERSION     DAOQDO010002146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124580_length_1323_cov_2.115931"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..480)
                     /locus_tag="PKZ53_25625"
     CDS             complement(58..480)
                     /locus_tag="PKZ53_25625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43887.1"
                     /translation="MLGETHTSTPGNKTKDNEILEELPGVDFFDFLPVEQEVVGIGLS
                     IPKGCRSDSRSVARLCELPPEKGHSPPLSTTARAPAGRGQWWKEKRWWPCPVVGPKRP
                     TDRLSERQLFEVQPKNLHVKEIGQNGARILQMEDKSDF"
     gene            459..>1323
                     /locus_tag="PKZ53_25630"
     CDS             459..>1323
                     /locus_tag="PKZ53_25630"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNJ43888.1"
                     /translation="MCAFLLTLTTALPVEAQRQGKGRGGRNRERETDEQFARSRRNQD
                     LLIERDEVEARELGKVWEYFSPDVTRLPVLVVNRLVCMPLQGGRVVALSLENGSLLWE
                     TPQGGEFTAPLSAGKSELYIATERQTETGKEGVLRALDTATGLTRWTKTFPHTFRSAL
                     IEVGDTLYTTMSDGKLHALQRTDGSSKWEFAAQGRFVIDHDQLLLGSDDNTLYALKLE
                     TGEPLWKFQGKGKIGKPACDDRQVYFGSADGRVRAILRTTGTLVWERRAGASVLAAPL
                     VKGNQLLVPSFD"
BASE COUNT          298 a          341 c          368 g          316 t
ORIGIN      
        1 acctgctgat acgattcaaa tctgtcaaat caaaattcga tcttatgaga tacacattca
       61 aaaatcgctt ttatcttcca tttgcaaaat ccttgcgcca ttttgcccga tttctttcac
      121 gtgcaggttt ttgggttgca cctcgaagag ttgccgttcg gatagccggt cggttggccg
      181 ctttgggcct accaccggac acggccacca cctcttttct ttccaccact gcccgcgccc
      241 cgcaggggcg cgggcagtgg tggagagggg cggggaatga cctttttccg gtggtagctc
      301 gcaaagcctc gcaaccgacc ggctatccga acggcagccc ttcgggatgc tcaagccaat
      361 tcccacaacc tcttgttcca ccgggagaaa atcaaaaaaa tccacaccgg gaagctcttc
      421 cagtatttcg ttgtctttcg ttttgtttcc tggtgttgat gtgtgcgttt ctcctaacat
      481 tgacgactgc cttgccagtt gaagcccaac ggcagggaaa aggccgtggc gggcgaaatc
      541 gggaacgtga aactgacgaa caatttgccc gttcgcgccg caaccaggac ctgttgattg
      601 aacgtgacga ggtcgaagcc cgcgaactcg gcaaagtctg ggagtacttc tcaccggatg
      661 ttacccgatt gccggttctg gtggtcaatc ggctggtgtg tatgccactc cagggcggac
      721 gggtggtggc gctcagtttg gaaaatggca gtttgctgtg ggaaacaccc cagggtgggg
      781 aatttacagc gccgttaagt gctggcaaat cagagcttta tatcgcaacc gaacgccaga
      841 ccgaaactgg aaaagaaggc gtgctccggg cgcttgatac ggccacggga ttaacgcgct
      901 ggactaaaac ctttccgcat acctttcgat ctgccttgat tgaagtcggt gataccctct
      961 acacaacgat gagtgacggg aaacttcacg ccctgcaacg cacagatggc agttccaaat
     1021 gggaatttgc ggcccaagga cggtttgtga ttgatcatga ccagcttttg ttgggtagcg
     1081 atgacaatac gctttatgcc ttaaaactcg aaaccggtga accactctgg aaatttcaag
     1141 gaaaaggaaa aatcggcaaa ccagcctgtg atgaccgtca ggtgtatttc ggttcggctg
     1201 atgggcgggt tcgggcgata cttcgaacca ctggaactct ggtttgggaa cgtcgggctg
     1261 gggcgtcggt gctggcggcg ccgctggtca aaggcaacca gttgcttgtg ccgtcatttg
     1321 ata
//
LOCUS       DAOQDO010002147         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124585_length_1323_cov_2.059148, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002147 DAOQDO010000000
VERSION     DAOQDO010002147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124585_length_1323_cov_2.059148"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /gene="metG"
                     /locus_tag="PKZ53_25635"
     CDS             <1..1269
                     /gene="metG"
                     /locus_tag="PKZ53_25635"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNJ43889.1"
                     /translation="VGKDILRFHAVYWPAFLLAADVPPPQRVFAHGMWLSKGRKMSKT
                     LKNTIDLSILLQFFTREQVRFFCLREMSYGQDGDFTYEALIDRINGDLAAGWGNLISR
                     TLTMVRNSCGGVLPNIPADAPADAVEAIEAVKVKFAENERDFAAELDRIATGRAMELV
                     LELVGTLDKYLSHYAPWKLAKDESARPLLLTVLNTAVEGLRHITVLMACAMPDAATEV
                     WKQLGLAGSPADYNPQALHWESLPEHTHIGELKGLFPRLNKEKVMAEIQEEVNKQSLI
                     AGASTDGEQQTPVAPVAEAAPVATAPVAEAAPAEEKGYITIDDFVKVELRVGQVLEAE
                     RIPKADKLLKLQVDLAEEKPRQILAGIAQYYEPEALVGRKIVVVANLAPRKMRGLESQ
                     GMLLAASIGSEGRPVLATFTEEVPNGARLK"
BASE COUNT          345 a          305 c          379 g          294 t
ORIGIN      
        1 gttggaaaag acatcctgcg gtttcacgcg gtgtactggc cggcattttt gttagcggct
       61 gatgtgccac cacctcaacg cgtgtttgcc cacggcatgt ggctgtccaa aggccgcaag
      121 atgtcaaaaa cattgaagaa cacgattgac ctgagcattc tgctccagtt tttcactcgt
      181 gagcaggtgc ggtttttctg cctgcgtgaa atgagctatg gtcaggacgg agactttacc
      241 tacgaggcgc tgattgaccg gattaacggc gatctggctg ctggttgggg aaatctgatc
      301 agccgcacgc tgaccatggt gcgcaattcg tgcggtggcg tgttgccgaa tattccagcg
      361 gatgctccgg cagatgctgt cgaagctatt gaagctgtga aagttaagtt tgccgaaaat
      421 gaaagagact ttgcggcgga actggatcgg attgcaactg ggcgagctat ggaactggtg
      481 ctcgaactgg tcggcacact cgataaatat ttgagccact acgcgccgtg gaaactggcg
      541 aaggatgaat ctgcgcgtcc gcttttgctc accgtcctca acacggcagt cgaaggtctc
      601 cggcatatta cggtgttgat ggcctgtgcg atgcccgacg ctgccactga agtttggaaa
      661 caactggggt tggctggttc gcctgcggat tacaaccccc aggctttaca ctgggaatca
      721 cttcccgaac atactcacat tggagaattg aaaggtctct tcccgagact gaacaaagaa
      781 aaagttatgg ctgaaattca agaagaagtg aataaacaat ctcttatagc tggggcctca
      841 acagatggag aacaacaaac ccctgtggcg ccagttgctg aagccgcgcc cgttgcgaca
      901 gcaccagttg ccgaagcggc gccagcggaa gaaaagggct acattacgat tgacgatttt
      961 gtcaaagtgg agcttcgcgt cggtcaggtg ctcgaagccg aacgcattcc aaaagctgat
     1021 aaactgttga aattacaggt tgatctggct gaagaaaaac cgcgtcagat tctggcgggg
     1081 attgcacaat attatgagcc ggaagccctg gttggccgca aaatcgtcgt cgtggccaat
     1141 ttggcacctc ggaaaatgcg cggactcgaa tcacaaggca tgctgctcgc cgcttcaatt
     1201 ggttcagagg gccgtccggt actggcaacg tttactgaag aagttcccaa cggagcaaga
     1261 ctgaagtagg ggaatgaaga atgaagaatg aagaatgaag aatgaagaat gaagaatgaa
     1321 gaa
//
LOCUS       DAOQDO010002148         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124590_length_1323_cov_2.010252, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002148 DAOQDO010000000
VERSION     DAOQDO010002148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124590_length_1323_cov_2.010252"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKZ53_25640"
     CDS             <1..615
                     /locus_tag="PKZ53_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Lon protease"
                     /protein_id="HNJ43890.1"
                     /translation="EEKFVVVPEQGGGSLIPDGPLSPGTLHTVATGGSSGHLGLYRIE
                     TQVTAGSGALKTSGLGSSGAARESVKIGFDYFKANAGRVSASIKPGDHDYHVHFVELH
                     NTGPTPTLTLATVIALCSAVLGKAVQSQFVVLGSMSLGGNIIPVENVAESYQVAADSG
                     GKRLLLPMASVRDIPTIPGELFAKFQTSFYSDPIDAVFKAFGMQ"
     gene            complement(700..>1323)
                     /locus_tag="PKZ53_25645"
     CDS             complement(700..>1323)
                     /locus_tag="PKZ53_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNJ43891.1"
                     /translation="SIVIDPHKHGLQPYGCGCVLFRDRNVGWLYRHDSPYTYFSSDEL
                     HLGEISLECSRPGAAAVALWATHQHFPLVRGGTFAGQLAKSRAAALTLFQKLKADSRF
                     LTAFEPELDIVVWIPKARNAYQVSALSRQIFAETARQGLHLALADLPVEFFNLKAAGI
                     EIDQPHITVLRSVLMKPEHLDWIEDIWRLLDQATTNCRSHQALVNHQ"
BASE COUNT          312 a          319 c          398 g          294 t
ORIGIN      
        1 gaagaaaaat ttgtcgtcgt gcccgagcaa ggtggcgggt cgctgattcc ggatgggccc
       61 ttgagtccag ggacgctcca cacggtggcc acgggcggga gttccgggca cctggggttg
      121 taccggattg aaacgcaggt caccgccggg tctggagcgc taaagacctc cgggttgggt
      181 tccagcggcg cggccagaga gtcggtcaaa atcgggtttg attatttcaa agcgaatgct
      241 ggccgggtga gtgcttcgat caaacccgga gaccacgatt accacgtgca ttttgtcgaa
      301 ctccacaaca cggggccgac gccaaccctg acgctggcga cggtgattgc actgtgttcg
      361 gcagtgcttg gaaaagcggt tcaatcgcaa tttgtggttt tggggagtat gagcctgggg
      421 ggaaatatca ttccggtcga aaacgtggcc gaatcatatc aagtcgctgc cgatagcgga
      481 ggcaaacggc tcctgctgcc aatggccagc gtgcgggaca ttccgacgat tcccggcgaa
      541 ttgtttgcca aatttcagac cagtttttat tcagacccga ttgatgcggt atttaaagcc
      601 tttggaatgc aatgaaacca gggaaaccag agaaaccacg gaatacacga ttccgtggtt
      661 aaatcgtgtt tcagaaggcc agttggcgga ttgagcgcgc tattgatgat tgacgagcgc
      721 ctggtggctc ctgcagtttg ttgtcgcttg atcaagaagc cgccagatat cttcaatcca
      781 atccagatgt tccggtttca tcagcactga tctgagcacg gtgatgtggg gttgatcaat
      841 ttcgattcct gcagctttga ggttaaaaaa ttcaactggg agatcagcca gcgccagatg
      901 taatccctgg cgagcagttt cggcaaaaat ctggcgtgac agcgcgctga cctgataggc
      961 atttcgggct tttggaatcc agaccacaat gtcgagttcg ggttcaaaag ccgtcaaaaa
     1021 gcgggaatca gctttgagtt tttgaaagag ggtcagggct gcggctcgcg atttggccaa
     1081 ctgaccggcg aatgttccac ctcgaaccag aggaaaatgc tgatgcgtgg cccacagtgc
     1141 aacggctgct gctccggggc gcgagcactc caggctgatt tcgccgaggt gcaattcatc
     1201 tgaactgaaa taggtatagg gcgagtcatg gcgatagagc cagccgacgt tgcgatcccg
     1261 gaacagaaca cagccacagc catagggttg caggccgtgt ttatgtggat caatcacaat
     1321 gga
//
LOCUS       DAOQDO010002149         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_124688_length_1322_cov_3.463299, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002149 DAOQDO010000000
VERSION     DAOQDO010002149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124688_length_1322_cov_3.463299"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            657..>1322
                     /locus_tag="PKZ53_25650"
     CDS             657..>1322
                     /locus_tag="PKZ53_25650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43892.1"
                     /translation="MTDTVKSDLWNKFCDEFRVRDEIVPLLDTIALSAQTMRYGRDER
                     LVLKRSAAMDNLVIREVKKVLLDFNSHTDKYAGLLYMMCWIRENQVYPLYIGKSEKFG
                     RQGNNLSANIKEIERNQGSFCRWGYNYAYHLGDLSAVVCPGHPAIKQTPKYRIWAERI
                     FQPGTWPSKNPVLCQPLWFWMTAWPTNAVGIWKEYGVTRLTFQEYLLIGLASEVFPDS
                     LLNI"
BASE COUNT          361 a          284 c          319 g          358 t
ORIGIN      
        1 attggataaa ctgtgtttgg gaaagtcctt ttggatagaa aagaggctgg ttatccattg
       61 cacgagcttg gctttagttt tatttgcaga ttaaccaaca agatttggat tgaaagctga
      121 gaagtagagc gaaaaacttt tgcacaaacc cgaataagga gtcaacttga aactctccac
      181 caattggatg ggggctgtac ttgaatggct ttgatttgtc gagagggaaa acaaggttcg
      241 ttcggttaag taattcaatc taaatgaact ttaaagcgga gcggagctta aatcagtgca
      301 gcaggtatat aatctcaaaa ctactttcct tgagattgaa ttctcctgaa gttaatccgt
      361 caatagaacc agtcagtaaa gccccggtga aagtcgggga tgacaacgat tcgtttttat
      421 cctatacggc tctttccaaa agaaatgccg acctcgcgca ggaaccgcat ccgtgtatgg
      481 cgaatttgga cgttttccca ttggtttgtt ttttcgaatg ctttagggta ccgaaacgcc
      541 ctttgttttc aactcaatac tggagttgcc agtactcaac catcaacacc ggtttaagct
      601 cagataaagt agctccccaa ttttatttgg attcggtcct ccactttcaa aagcgtatga
      661 cagacaccgt caaatcggac ttatggaaca aattctgtga cgagtttcgg gtccgtgacg
      721 aaatagtgcc actgctggac accatcgccc tatcggcaca aaccatgcgc tatggccggg
      781 atgagcgcct ggtcttgaaa cgatcagccg caatggacaa tctggtgatt cgagaggtga
      841 aaaaggtcct gctcgacttt aactcccaca cggataaata tgcgggcttg ctctatatga
      901 tgtgttggat cagggaaaac caggtgtacc cgctctacat tggcaaatca gaaaaatttg
      961 gtcggcaggg aaataattta tcggccaata tcaaagagat tgagcgcaat cagggcagtt
     1021 tttgtcggtg gggttacaat tatgcctatc atctgggcga tttgagcgct gtcgtctgtc
     1081 caggtcatcc tgcaatcaaa caaacgccga agtaccggat ctgggctgaa cgaatctttc
     1141 aacctggaac ctggccgagt aaaaatccag ttctgtgtca gccactttgg ttttggatga
     1201 cagcctggcc cacgaatgcg gtcgggatct ggaaagaata tggagttacc cgcctgacgt
     1261 ttcaggaata cctcctgatt ggcctggcga gtgaggtttt ccctgattca ttgctcaata
     1321 tt
//
LOCUS       DAOQDO010002150         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125002_length_1321_cov_1.814376, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002150 DAOQDO010000000
VERSION     DAOQDO010002150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125002_length_1321_cov_1.814376"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PKZ53_25655"
     CDS             <1..1027
                     /locus_tag="PKZ53_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ43893.1"
                     /translation="YQSTTRSLLGIPPIRRDLIKRHLYLVPNSIVVSRGCPHSCDFCY
                     KEAFFTGGKGFYTQLVDDALAEIERLPGRHLYFLDDHLFGNVKFATELFEGMRGMNRL
                     WQAAGTVQSVLRPELLEKAVASGLRSLFVGFETLSQKNLRDQQKPQNLNRDYTAAIRR
                     LHDQGVMVNGSFVFGMDDDDPGVFDRTVEWAIDQGIETATFHILTPYPGTALYQRMEA
                     EGRILHSNWDLFDTRHVVFRPTKLSAQELEEGYWRAYQNFYRWSSIFQGAATKPGLRE
                     KLRHVAYAGGWKKFEPLWDVIIRARRVSTMLPVLEQVLTGLGIHRPQKRSGESASTMS
                     LIKHVPG"
BASE COUNT          323 a          332 c          323 g          343 t
ORIGIN      
        1 gtaccagtca acgactcgct ccctgcttgg aattcctccg attcggcgtg atttgatcaa
       61 acgccacctc tatttggtgc caaactcgat tgtggtttca cgcggttgcc cccattcgtg
      121 cgatttttgc tataaagaag ccttttttac cggcggaaaa gggttttaca cgcaactcgt
      181 tgatgatgcg ctggccgaaa ttgaacgcct tcctggacgt cacctgtatt ttctggacga
      241 tcacctgttt ggaaacgtca aatttgccac cgaactgttt gaaggcatgc gcggcatgaa
      301 tcggctctgg caggccgccg gcacggtcca gtcggtgtta cgccctgaac ttttggaaaa
      361 agcagtcgcc tcaggtctac gcagtctgtt tgtcggattt gaaacactca gtcaaaaaaa
      421 tcttcgggat cagcaaaaac cccagaatct gaatcgtgac tacaccgcag ccattcgtcg
      481 cttgcacgat caaggagtga tggtcaacgg gagttttgtg tttgggatgg atgatgatga
      541 tcccggcgtc tttgaccgca ccgtcgaatg ggcaattgac cagggcatcg aaaccgcaac
      601 ctttcacatt ttaacgcctt atcccggaac cgccctgtac cagcgaatgg aagccgaagg
      661 cagaatactc cattccaact gggatctttt tgatacccga cacgtcgtct ttcgaccgac
      721 caaactttcg gctcaggaac ttgaagaggg gtactggagg gcgtaccaaa acttttatcg
      781 ctggagttca atctttcagg gtgccgccac caagcccggt ttacgcgaga aactccgaca
      841 cgttgcctat gccggaggct ggaaaaaatt tgaaccgctc tgggatgtga ttatccgggc
      901 acgtcgggtt tcgacaatgt tgccagtgct ggaacaggtc ctgaccggcc tcggcattca
      961 tcggccacag aaaagaagtg gtgaatccgc gtccacaatg tcgctcatta aacacgtgcc
     1021 tggctagtgt ttctgttctg aaacaatcag gactcatctg aacagacttt tgaagagagt
     1081 tggtgaaaca tctgttttga gatgcctcac tggctctttt ttattcaact ccaggcaaaa
     1141 acggtttctc cgcctcaaaa tctaccagca attgcccggt atcaattgac accttgtcct
     1201 gtttcagtcg ttgaatagag ttgtgatgat attaagaatc aatgagttag atgaaagttc
     1261 cagagtccag ggcaaatatc caaaatggtg ctgtagcgtt tgatgtcttg aactcaacgc
     1321 c
//
LOCUS       DAOQDO010002151         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125115_length_1320_cov_2.736759, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002151 DAOQDO010000000
VERSION     DAOQDO010002151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125115_length_1320_cov_2.736759"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKZ53_25660"
     CDS             <1..740
                     /locus_tag="PKZ53_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43894.1"
                     /translation="LADWKLTEETLHFYKNPKFGQRIMQLALPLYSAVDDMQEDDLSP
                     EERAYHDLGLHLERQLAERGMIPTVAGRLVESMLTQGKLDAIEKSISFFDRHYRTRKL
                     INPGGFLYTLIVNDLSGREREEPAPAPSSAKSNKFPESPALEEITDPEHLQLLELGYI
                     DYLERTGEELLVRMRKTELKQRIDQEREKLLASDKRRIYERWSAEVLSDHLRRLVGRS
                     LAAQQAESFASWCAQQGVILSTEGADE"
     gene            813..1097
                     /locus_tag="PKZ53_25665"
     CDS             813..1097
                     /locus_tag="PKZ53_25665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43895.1"
                     /translation="MAFKGDLSDFFTWHGVNGCFEGEKALYGQINAFGGCFEVEFNPG
                     FLVQFRFQRLSSHRFRKAHLARMRLGRNVLTEKNIYWFSVEMNFWWMFMR"
BASE COUNT          311 a          302 c          364 g          343 t
ORIGIN      
        1 tcctggctga ctggaagctg accgaggaaa ccctgcactt ttacaaaaat ccaaaattcg
       61 ggcagcggat tatgcaactg gccctcccgc tctatagcgc ggtggatgat atgcaggaag
      121 acgatctgtc acccgaagaa cgggcctatc acgatctggg gctgcatctc gaacgccaac
      181 tggccgagcg gggaatgatc ccaaccgtcg ctggccggct ggtggaaagt atgctcaccc
      241 agggcaaact cgacgcgatt gaaaaatcaa tcagtttttt tgaccggcac tatcgcaccc
      301 gcaaactgat taacccgggc gggtttctct acaccttgat cgtcaacgac ttgagtggtc
      361 gcgagcgtga agagcctgcc cctgctccat ccagtgccaa atccaacaaa ttcccagagt
      421 cacctgctct ggaagaaatc accgaccctg agcacctcca gttgcttgaa ctggggtaca
      481 ttgactatct ggagcgaact ggcgaggagt tgctggtgcg gatgagaaaa actgaactca
      541 agcagcgtat cgaccaggag cgggaaaagt tgctggcttc cgacaagcgc cggatctatg
      601 aacgctggtc cgccgaagtg ctcagcgatc atctgcgccg gctggttggc cgcagtctgg
      661 cggcgcagca ggccgaatcg tttgcttcct ggtgtgccca gcagggggtc attctgtcca
      721 cggaaggtgc tgacgagtaa ttgtgccggg acaggaccgt gggggacctg aagccactgt
      781 gagctggggg caacgtggga gaagttacct gtttggcgtt taaaggcgat ttaagcgatt
      841 tttttacctg gcatggggtg aatggatgtt ttgagggcga aaaagcgcta tacgggcaaa
      901 ttaacgcatt tggtggctgt tttgaggtag agttcaaccc aggattttta gttcagttca
      961 ggtttcaacg cctatcttcg catcggttca gaaaggctca tcttgcccgg atgagattgg
     1021 gtagaaacgt attaactgaa aaaaatattt actggttttc ggttgagatg aatttttggt
     1081 ggatgtttat gcgatgataa ctccttttat tttcaaatgg atcaattttt gctcttttgt
     1141 gtttttgcgt aataactctt tttttgaaaa ggcaatgatg atgtatatga cctctcttct
     1201 taagaaaaaa ctggctggaa ttcaaaaact caaatgtctg gtattgattc tggtctggtg
     1261 tttttcaggg ggaactcgcc ccgttttggc tggatcggtt ccaggtttgg ctcacaccct
//
LOCUS       DAOQDO010002152         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125140_length_1320_cov_2.498024, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002152 DAOQDO010000000
VERSION     DAOQDO010002152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125140_length_1320_cov_2.498024"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKZ53_25670"
     CDS             <1..781
                     /locus_tag="PKZ53_25670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNJ43896.1"
                     /translation="GATAIADLSKRLEKDLPALSEHLVGHAADEFRHAEMIAGMMADM
                     GATPTEPAGLRYVDEFEALTMGITDDSAPDEVEIIVGINVTEKRGLKSFALHVAAMGE
                     DHKHYPVLNQIKNEEAGHVRWGNDYIKDFQKRGLGEKVKQAQAKFNKIETAAYETSLD
                     IMPLAPIRRFGRILDIAQELPVEQRTGYVIEQFLRAADPIPLAKARWEFVSNVFSSPR
                     MRDQISDDISRLVTGQQLKDDSILGRIGTDAWNAVRSMFPA"
BASE COUNT          353 a          318 c          309 g          340 t
ORIGIN      
        1 aggcgcgacc gccattgctg acctttccaa acggcttgaa aaagacctgc cggcgctttc
       61 agaacacctg gttggacatg ccgccgatga atttcgccat gcggagatga ttgccggcat
      121 gatggccgat atgggtgcca caccaactga accggctggt ttgcgctatg ttgatgaatt
      181 tgaagcgttg acgatgggaa ttactgacga ctcagcgccc gatgaagtgg aaattatcgt
      241 cgggatcaac gtgactgaaa aacggggact aaaaagcttt gcactccatg tagcggcgat
      301 gggtgaagac cacaaacatt atcccgtcct caaccagatc aaaaacgaag aggccgggca
      361 cgttcggtgg ggaaatgact acattaaaga ctttcaaaag cgtgggttgg gagaaaaagt
      421 aaagcaagcc caggcgaaat tcaacaagat tgaaaccgcc gcttatgaaa cgtcgctcga
      481 cattatgccg ctggcgccga ttcggcgctt tggacggatc ctcgacattg cccaggaact
      541 cccggtcgaa cagcgcaccg gatatgtgat tgaacagttc ttgcgagccg ccgatccgat
      601 tcccctggcc aaagcccgtt gggaatttgt ctccaatgtg ttttcttcac cccgcatgcg
      661 cgaccagatt tccgatgata tcagccgact cgtcaccggg cagcaactca aggatgattc
      721 gattttgggt cgcatcggca ccgacgcctg gaatgccgta agatcaatgt ttccagcctg
      781 attggtcaga ttttcctggt cagaaaaaaa gttcagagga acccgttcag gtgtgagtgg
      841 aatccacaaa acgtgggtca caaatgctcg cctgtgcgcg tgagactctg aacttttact
      901 tttttcagcg ttgtgaaatc caatccaaaa ttccctcccg taacagcccc cgatctcaac
      961 tctatctgaa tttcacctca aattgaattt gatagattcc ctggttcgca ccaaaatcca
     1021 tctcaacagt gattataaat atttttgaca tattggagtg accttttgct tcgattatca
     1081 agatatattt tgagttaaat cttatttaag atatttaaaa tcaataattt agcttggttt
     1141 tttatatatt ttaccgagaa tctaatcaga ttttgaactg gtttatactg ctgctatcgg
     1201 atcttgtatt ggaggcaaat cagaaaaacc gagagatcag tagcacccgt gtcaatggat
     1261 gggcccgttt gcctccaacc cccacccgct cacgcaggtg gtattgacaa gtcctttatt
//
LOCUS       DAOQDO010002153         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125285_length_1319_cov_3.237342, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002153 DAOQDO010000000
VERSION     DAOQDO010002153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125285_length_1319_cov_3.237342"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..>1319
                     /locus_tag="PKZ53_25675"
     CDS             87..>1319
                     /locus_tag="PKZ53_25675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43897.1"
                     /translation="MQLVAIQECLKQAMATVSSHKFRSLLTVLGVIVGTTTTIAVTSI
                     ISGMNVRVTEMVERFGTNIAFISKWNGGPNFNGSNREEELRKELTLEDGQAIKQLPHV
                     ESAVACLGAWPGGPDAPVIKYRTNESKQSVVRGGEASFFDARSMVLAKGRLFGESEDL
                     HSAPVAVIGFDLAEALFPGLEPVDKEITINGHLYRVIGVLEKSSARGLFGGNNNWEDN
                     AAIIPLNSFSRMYPQEKDYVIVAKAKSGYLDKMVDEITELLRRRRKVPFNEPDNFGIS
                     TAQSNIDGFNKISEVLGWMVIPISAVGLLVGGIGVLNIMLVSVTERTKEIGIRRALGA
                     RRFDIILQFLIEAMSLTGLGGVMGILVGLFISWFLNTFVPSVPSFVSVFWIMVGFLVA
                     VSVGLISGLWPAIKAAYVD"
BASE COUNT          317 a          314 c          357 g          331 t
ORIGIN      
        1 aagaaccaag aaccaagaac caagaaccaa gaaccaagaa ccaagaacca ggcaccaaaa
       61 accctgaacc ctgaacccga aagcctatgc aactcgtcgc catccaagaa tgcctcaaac
      121 aagccatggc cactgtttca tcccacaagt tccgttcgtt gttgacggtt ctgggcgtga
      181 tcgttggcac caccaccacc attgccgtga cctctatcat cagcgggatg aatgttcgtg
      241 tcactgaaat ggtcgaacgg tttgggacca acattgcttt tatctcaaag tggaatggtg
      301 gcccaaattt taatgggtcc aatcgtgaag aagaactgcg caaagaactg acgctggaag
      361 atggacaggc aattaagcaa cttccgcacg ttgaatcggc ggttgcttgt ctgggggcct
      421 ggccgggtgg tcccgatgca cctgtgatca aatatcggac caatgaatca aaacaatccg
      481 tcgttcgcgg tggggaagcc tcattttttg atgcccgcag catggtgctc gccaagggcc
      541 ggttgtttgg tgaaagcgaa gacttgcaca gcgcaccggt cgcggtcatt gggtttgacc
      601 tggccgaagc gctctttcca ggactggagc cggttgacaa agaaatcacc atcaacggcc
      661 atctctatcg ggtgattggc gtccttgaaa aaagttcagc ccgtggactc tttggtggca
      721 ataacaactg ggaagacaat gccgctatta tcccgttaaa ttcatttagc cggatgtacc
      781 cacaggaaaa agattatgtg attgtggcca aagccaagtc tggttatctc gataaaatgg
      841 tggatgaaat cacagaatta cttcgacggc ggcgcaaggt tccattcaac gaacccgata
      901 acttcgggat ttcaacggct caatcaaata ttgacggttt caacaaaatc tctgaagttt
      961 taggctggat ggtgattccg atttcagccg tgggattgct ggtcggtggg atcggcgttc
     1021 ttaacatcat gctggtttcc gtcactgaac gcaccaagga aatcggtatt cggcgtgccc
     1081 tcggtgcccg tcggtttgac attattctcc agtttttgat cgaagccatg agcctgaccg
     1141 ggctgggcgg cgtgatgggg attctggttg ggttgtttat tagctggttt ctcaatacct
     1201 ttgtcccgag cgtgccgtcg tttgtgtctg tcttttggat tatggttggg ttcctggtgg
     1261 cggtgagtgt tgggttgatc tccgggttgt ggccagccat caaagcggcg tatgtggac
//
LOCUS       DAOQDO010002154         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125521_length_1318_cov_2.359462, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002154 DAOQDO010000000
VERSION     DAOQDO010002154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125521_length_1318_cov_2.359462"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKZ53_25680"
     CDS             complement(<1..917)
                     /locus_tag="PKZ53_25680"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNJ43898.1"
                     /translation="MLRLMTRYKRSQNLILVGIIVVLAVGMFIAYGTPSGWSKSGGPK
                     VVEPVADSTVIAEVDGRDITAKDYADGIRRYLAMYQMYRSQSPSSALPTDVKGLRDRG
                     TDKELLRSLVRDKIIEAEAERLALTATEDELRDRIREMYSPDGKWIGYEKFKRAIERQ
                     GSTVYEFEQSLRRQITEDKLRKFVTDGIQVSSREIEENYKQSNTKFQVTFTVLEEKSY
                     LDKVQAPTDQEVKAYFDSHKDAFRATKDQRKIQYLFISQEKAGETVPVTDEELRKEYD
                     PEKQVKSIKVKQIVLNVLTPKDEDTVRTKA"
BASE COUNT          309 a          321 c          328 g          360 t
ORIGIN      
        1 gccttggtgc gcaccgtgtc ttcatccttg ggggtcaaca cattgaggac aatctgcttg
       61 accttgatgg atttgacctg tttttcagga tcatattcct tacggagttc ttcatcagtc
      121 accggcacgg tttcaccagc tttttcctga ctaatgaaca gatactggat tttgcgctgg
      181 tctttggtgg cacggaacgc gtctttgtgg ctatcaaaat aggctttgac ttcttgatca
      241 gtcggcgcct gaactttatc gagatagctc ttttcttcaa gaacggtaaa ggtgacctga
      301 aatttggtgt tggattgctt gtagttttct tcaatttcgc gtgaactcac ctgaatgcca
      361 tcagttacaa acttacgcag tttgtcttca gtgatttggc ggcgcaggct ctgttcaaat
      421 tcatagacgg tactgccctg gcgttcaatg gcccgtttga atttttcata accgatccat
      481 ttgccatcgg ggctgtacat ttcacggatc cgatctctca gttcatcttc ggttgccgtc
      541 agggcaagcc gttcagcttc ggcttcgatg atcttatcgc gaaccagaga gcgcaggagt
      601 tctttgtcgg tgccacggtc acgcagtccc ttgacgtcgg tgggaagcgc cgaagatggc
      661 gattgagagc ggtacatctg atacatcgcc aggtaccggc gaatgccatc agcatagtct
      721 ttggctgtga tatcacggcc atcaacttcg gcaatcacgg tgctatcagc cacgggttca
      781 accactttgg gaccgccgct tttgctccag ccgctggggg tgccgtatgc aataaacata
      841 ccgacggcaa gaaccacaat aatgcccacc agaatgagat tctgggaacg cttgtagcgt
      901 gtcatgagac gtaacataac ttggtgaaaa cctccggaaa ccatctgccg ggagagagtg
      961 gcagatacag tcaaaataga gcttttgagg aaaacgggaa caataccgta aaagctgatt
     1021 tgcgcgcaat agaaagactg agttcaggtg gaaacacgtg gcttggggct tgggggcgga
     1081 agacagcggg gtcaatatcc ttagccccca gcccagattc ttcagccccc agccctatct
     1141 ttatggtttc tttattaacc ccttacacca tttttatgta gtggcgagta agctcaggtt
     1201 catacagaaa ggctgccctg acctgatggg ttcaatgtag agacttcctc cacggtcagg
     1261 agctgaaaac ttatgaacct tttttccaaa tctcacacat tgattcaaat tgccggat
//
LOCUS       DAOQDO010002155         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125858_length_1316_cov_4.042823, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002155 DAOQDO010000000
VERSION     DAOQDO010002155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125858_length_1316_cov_4.042823"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PKZ53_25685"
     CDS             <1..762
                     /locus_tag="PKZ53_25685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43899.1"
                     /translation="STKHQAPSTKHQAPSTKHCVMLNSINAKKIETGKYSMFSWLREA
                     WDQSNLAFEALEELQHDNSLARLAQGQLRVSEQKVNELLAEAKLEGVRQALVDVEEGF
                     FSLSAQSERFFLARLTIPLRVEKIIFTPYRHRLYLVQAGNVSAHGSTLWQRATAYTVL
                     ALLHHILSPNAPIHSLSDPSEGFTVRGPHLEIDLHRIPEARQVLQRQYEILGKQICPL
                     RFVTIDSIRCYPQYFEINSSVNWDDLYTHLREVIF"
     gene            861..>1316
                     /locus_tag="PKZ53_25690"
     CDS             861..>1316
                     /locus_tag="PKZ53_25690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43900.1"
                     /translation="MLIETITSRQNPFVKRLISARQGRERHMLFVEGIRLVEDLLNTS
                     LHVDAIAYSPVLHDTDRGQALYDRIQTLPCRGTLLTEKLMHSVSGVETPQGIIALAHR
                     LHFDLTDVIEDVTPQLLVFADGLQDPGNLGALIRTAEATRTTGVITSRQS"
BASE COUNT          324 a          385 c          298 g          309 t
ORIGIN      
        1 agcaccaagc accaagcacc aagcaccaag caccaagcac caagcaccaa gcactgcgtt
       61 atgctcaatt cgatcaatgc taaaaaaatt gaaacaggaa aatactctat gtttagctgg
      121 ttgcgtgaag cctgggacca aagcaatctg gcgtttgaag cccttgaaga attacagcac
      181 gacaattcgc tggccagact tgcccagggt caactccgag tatcagagca aaaagtgaac
      241 gaattgctgg ctgaggccaa attggaaggc gtccggcagg cactcgttga cgtcgaagaa
      301 ggcttttttt cactttcagc ccaaagcgaa cgcttttttc tggcccgatt gaccattccg
      361 ctccgggtcg aaaaaatcat cttcacgccc tatcgccacc gcctgtatct ggttcaggcc
      421 ggaaatgttt ctgctcacgg ctcaacactg tggcaacgcg ccacggccta tacggtactg
      481 gcgttgcttc atcacattct cagtccaaac gccccgattc attcgctttc cgatccatcg
      541 gaaggattca ctgttcgcgg cccacatctg gaaattgacc tccatcgcat ccccgaagcc
      601 cgacaggttc tgcagcgcca gtatgaaatt ctcggcaaac aaatttgccc tctgcgcttt
      661 gtgacgatag attccatccg ctgttatccg caatatttcg aaatcaactc gtctgttaac
      721 tgggacgatc tctataccca cctgcgggaa gtcatctttt gaataaaatc acaagtcagt
      781 accacctgcg tgagcgggtg ggctcctttc ttcctaaccc acccgctcac gcaggtggta
      841 ctgacctccc cttacttgcc atgctgattg aaaccataac cagtcgccaa aatccatttg
      901 tcaaacgatt gatcagtgcc cgccagggtc gagaacgcca catgctgttt gtggaaggca
      961 ttcgattggt cgaagacttg ctcaacacct cgcttcacgt ggatgcgatt gcctatagcc
     1021 cggtgctgca tgacactgac cggggacagg cactctatga ccgcatccaa acccttccct
     1081 gtcgtggcac cttgcttacc gaaaagctga tgcacagcgt gtctggggtt gagaccccac
     1141 agggtatcat tgcgcttgcc catcgcctcc actttgatct gactgatgta attgaagatg
     1201 tgacaccgca actgcttgta ttcgctgatg gattacagga tccgggcaat cttggagcgc
     1261 tgattcgaac cgccgaagcc acccgaacca caggtgttat tacctcccga cagtca
//
LOCUS       DAOQDO010002156         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125869_length_1316_cov_3.561459, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002156 DAOQDO010000000
VERSION     DAOQDO010002156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125869_length_1316_cov_3.561459"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..>1316
                     /locus_tag="PKZ53_25695"
     CDS             218..>1316
                     /locus_tag="PKZ53_25695"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNJ43901.1"
                     /translation="MHKTLLALTSRFESTQPHHRLWWVKILILVVFALATVGVTWTHN
                     HTDAATGTKSQKLPVAIPKGIPVDLFESLIPKDNPLTPEKIALGEKLFFDKRLSADRT
                     VSCATCHDPAMGFADGNMVGIGIELKKGARNSPTILNAMFNEAQFWDGRAPSLEEQSK
                     LPLINPLEMGMKDHAQVVERVKEVPEYKAEFTKVFGNEGITIDTIARAIASFERTQLS
                     GDAPFDRFIAGDQNAISESAKRGWELYNGQARCISCHAFNPSNPFFTDFKFHNIGVAA
                     KDQNFPALARKSRQLIAQGKDSAQVVDELALQPGFSELGRYLITKQPKDIGAFKTSML
                     RDIELTAPYMHNGSEKTLLDVVKFYDKGGELN"
BASE COUNT          338 a          363 c          297 g          318 t
ORIGIN      
        1 atccccgatg ttttcagccc gaaatgcttc aatccagctt tttgaacttt tttccgattt
       61 tgacggaata aacccgcccg ttgagtgttc ttacctccag atcaactcca aacaagtcat
      121 tttcctaccg ccgatttgtt tggagggcat gccgcttcac acgctttcca atttccgcaa
      181 tttcaagctt cacaattcca caacgaaggg gcccgctatg cacaaaactc ttctggcgct
      241 cacatcgcgg tttgaatcca cccaaccgca tcaccggctg tggtgggtga aaatactcat
      301 actggttgtg tttgcactcg caaccgtcgg agttacctgg acccacaatc acactgatgc
      361 cgccaccggg acaaaatcgc aaaaattacc cgttgcgatt cccaaaggaa ttccggttga
      421 cctgtttgag tcgttgattc caaaagacaa cccactgact cctgaaaaaa ttgccctggg
      481 tgaaaagctt ttctttgata aacgcctgtc tgctgaccga acggtgagtt gtgccacctg
      541 ccacgacccg gcgatgggtt ttgccgatgg aaatatggtt ggcatcggca tcgagttaaa
      601 aaaaggggcg cgtaattcgc caaccatcct caacgccatg tttaatgagg cacagttctg
      661 ggatggccgc gcaccatcgc ttgaagaaca atccaaactg ccgcttatca acccactgga
      721 aatgggcatg aaagatcacg cccaggttgt cgaacgcgtc aaagaagttc ccgaatacaa
      781 agctgaattt accaaagtct ttggcaatga aggtattaca attgacacga ttgccagggc
      841 gattgcttcg tttgaacgaa cccagctttc aggggacgcc ccctttgacc gctttattgc
      901 cggagaccag aacgcaattt ccgagtcagc caaacgcggc tgggagttgt acaatggtca
      961 ggcgcgctgt atttcgtgtc atgcctttaa cccgtccaat ccattcttca cggatttcaa
     1021 attccacaac atcggcgtgg cagccaaaga tcagaacttc cccgccctgg cccgcaaatc
     1081 acgccaactg attgctcaag gcaaagacag cgctcaggtg gttgatgaac tggccttgca
     1141 gcccggtttt tcagagcttg gccggtattt gatcaccaaa cagcccaaag atattggcgc
     1201 tttcaaaact tcgatgctgc gtgacattga attgaccgcc ccctatatgc acaacgggtc
     1261 ggaaaagacg ctgctcgatg tcgtcaaatt ttatgacaaa ggcggtgaac tcaacc
//
LOCUS       DAOQDO010002157         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125908_length_1316_cov_2.586836, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002157 DAOQDO010000000
VERSION     DAOQDO010002157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125908_length_1316_cov_2.586836"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKZ53_25700"
     CDS             <1..671
                     /locus_tag="PKZ53_25700"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNJ43902.1"
                     /translation="IGGYQVDDQGVAARPVPLVENGILKTLLMSRRPRKGIDRSNGHG
                     RAVPFAGINATISNLFIQPREGKSEAEIKQELIALCKQENLPFGIWVKVLSVPGGWTD
                     VNDAGDAANSYQREGLTAPVSIYKVYVDDGREELVRGIKIGGVSFRQFKRLSAIGQEV
                     YVHNRIATGGGASIDIGQGSAGLLGLPTTVVAPSVLIEDVELRPITGPRQKPVLLTHP
                     YFSR"
     gene            797..1039
                     /locus_tag="PKZ53_25705"
     CDS             797..1039
                     /locus_tag="PKZ53_25705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43903.1"
                     /translation="MYRDLLNFLASSPTPHEIAAFQPDPKVQQRLHLLLQRERDAGLT
                     PSEKAELDEYERIEHLVVMLKAGNLISVQSSSFSLQ"
BASE COUNT          304 a          326 c          371 g          315 t
ORIGIN      
        1 tgattggagg atatcaggtt gatgatcagg gcgtggcggc cagacccgtg cccctggttg
       61 aaaacggcat cctcaaaacg ttgttgatgt cgagacgacc tcgcaaaggt attgaccggt
      121 caaacggcca tggtcgagct gttccatttg ccggtatcaa tgccaccatc agtaatttgt
      181 ttatccagcc cagggaaggg aagtcagagg ctgagataaa gcaggaattg attgctctgt
      241 gcaaacagga aaacctgccg ttcggaattt gggttaaggt gttaagcgtg ccgggaggct
      301 ggacagatgt gaacgacgcc ggcgatgcgg ccaatagcta tcagcgcgaa ggactgacgg
      361 ccccagtcag tatatataag gtgtatgttg atgatggacg cgaagaactg gtccggggga
      421 tcaagattgg cggagtttcc ttccggcaat ttaaacggct ttccgccatt ggccaggaag
      481 tgtatgtcca caatcgaatc gccaccggcg gcggggcctc gattgatatt gggcaaggct
      541 cagccggatt gcttgggttg ccgaccacgg tggtggcacc gtcagtcctg attgaagacg
      601 tcgaactccg gcccattacc ggtccacggc aaaaaccagt gctcctgacc cacccttatt
      661 tcagccggta atggttgttg atggtgccgg tttgtagtca gtggttcatt aagtttattt
      721 gactgaatca cttgaatgtt ttctgatacg acgcattcat tgcaaaatgg tatcaactca
      781 atggaggttt ccgtcggtgt accgcgatct gttaaatttt ctggcatcaa gcccaacacc
      841 acacgaaatc gctgcctttc aacctgaccc aaaagttcag caacggcttc acctgctact
      901 tcaacgcgaa cgagatgctg gcttgacacc ctctgaaaaa gctgaactgg atgaatatga
      961 gcggattgaa cacctggttg tgatgctcaa agctgggaat ttgatttctg tacagagttc
     1021 aagctttagc ttgcaataac tccgttgcgg aaaggccact tgagcttgac aacccccgcc
     1081 cggagggcga tggaaaatag ccggtggtcg cgaggctttg ggagctacca ccggatcagt
     1141 gtccagttca gtcccgcgcc cggatgggcg ctggaacatc ttatcggtcc gggattctca
     1201 attcctcgac gcttttgaga ttttgatcca gcgcccatcc gggcgcgagc cattgggtga
     1261 ctgaattccg gtgggtgcgc ctcaaagcag gctgcccacc ggctattttc cggcgc
//
LOCUS       DAOQDO010002158         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_125925_length_1316_cov_2.430611, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002158 DAOQDO010000000
VERSION     DAOQDO010002158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125925_length_1316_cov_2.430611"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1316
                     /locus_tag="PKZ53_25710"
     CDS             <1..>1316
                     /locus_tag="PKZ53_25710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ43904.1"
                     /translation="VAQGKMEDEGWRVKKDGTRYWANEVITPLFDDNGTLVGFSKVLR
                     DLSERHTQEQKIQEQKLILAGIIDATMDGVITIDDTHRIIVFNRASEAMFRCSAAEAL
                     GQPLDTFIPERFRTSHDQLVRNFADSGTCSQKMAVPRIIYGLRADGEEFPLEAVVSQT
                     AVNGRKQLTVIVRDITQRLQSENAITAQLALQEQLAKLAEVFPGALFSFCQKSDGAFA
                     MPYISPSVEEVFGLTADLIREDATPIFQGIHPDDVALVRQSIAESARLMTLWHDAFRF
                     QHPDKGELWLEAWSAPVHELDGGILWYGVVIDATSQKQIKAELIENQRYLKQLIESLP
                     QIVWTTNNQGQCTYVSPQWYDYTGIQPITPLPETWSHLVDPEDSERNAGPFQEALHIG
                     QMFDAEYRLRRADGAYRWFKVRAVPFRDQSGKIVKWIGTSTDIDDQ"
BASE COUNT          338 a          326 c          347 g          305 t
ORIGIN      
        1 cggttgccca gggcaaaatg gaagatgaag gctggcgggt caaaaaagat ggtacgcgct
       61 attgggccaa tgaggtgatt acgccgctct ttgatgacaa cgggacattg gttggcttta
      121 gcaaagtgct gcgtgatctc tcagagcgcc acacccagga acagaaaatc caggaacaga
      181 aactgatcct ggctgggatt attgacgcga cgatggatgg cgtcattaca attgatgata
      241 ctcatcggat cattgtgttt aaccgggcat cagaagccat gtttcgatgt tcggcagccg
      301 aggcgcttgg ccagcccctc gataccttta ttcccgagcg atttcgcacc tcccatgacc
      361 aacttgtccg gaattttgcg gattcaggta cttgctccca aaaaatggca gtgccccgca
      421 ttatttatgg gttacgggcc gatggagaag aatttccact tgaagccgtt gtttcacaga
      481 cggctgtcaa cgggcggaaa caactaacgg taattgtgcg cgacatcact caacgcctcc
      541 agtccgaaaa cgccataacc gcacaactgg cgctacaaga gcaattagcg aaactggcgg
      601 aagtctttcc cggcgccctt ttttcctttt gccaaaaatc tgatggggcg ttcgccatgc
      661 cttacatttc accatctgtt gaggaagtat ttgggttgac ggctgatctg attcgggagg
      721 atgccacgcc gatttttcag ggaattcatc ctgatgatgt cgctcttgtc agacaaagca
      781 tcgcggaatc cgccaggttg atgacgctat ggcacgatgc tttccgattt caacatccag
      841 acaaaggtga actctggctt gaagcctggt cggcgccagt ccacgaactg gatgggggca
      901 ttctgtggta cggggtggtc atagatgcca ccagccaaaa acagattaag gctgagctga
      961 ttgaaaacca acgatatctc aaacagttaa ttgaaagctt gcctcaaatt gtgtggacca
     1021 ccaacaatca gggacaatgc acctatgtca gtccacaatg gtatgactac actgggattc
     1081 aacccataac cccgttgcct gaaacctgga gtcatctcgt tgacccagag gatagcgagc
     1141 gaaatgcagg accatttcag gaagctctcc acatcgggca gatgtttgat gccgaatatc
     1201 ggctccgccg ggcagatggt gcctatcggt ggttcaaggt gcgagcggtg ccattccgcg
     1261 atcagtcagg aaagatcgtg aaatggatcg gcaccagtac tgatattgac gaccaa
//
LOCUS       DAOQDO010002159         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126040_length_1315_cov_4.426984, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002159 DAOQDO010000000
VERSION     DAOQDO010002159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126040_length_1315_cov_4.426984"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PKZ53_25715"
     CDS             complement(<1..1224)
                     /locus_tag="PKZ53_25715"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ43905.1"
                     /translation="MSKQLVKGQRIKLSDLTPQSQIRLDIALSGLKDHAQFYCLALNE
                     QQKLLKSEYLVFSNQPKSPCQSIQIASTTPTQTSINVNLSSLPGEAKEVVLALSLGER
                     TGCLLENASDIQSGSLVVSAGSQEVARYQFSAQDFDGEKALVLGRLYQKDGWRLGLVS
                     SGFRAGQSALFDHFGVPAGLVAPVAPPPPPPQAYGQPAGAAQPAPAPQPPLEDQPILL
                     PGKLPPPKPVYMPASFPGNQSPTVPGGLTGAVGFILVECQDGQHASGTGFVINPGGFI
                     LTCYHVVSDAKRMDICLSGTNYLRPLLYVAGSAQHDLALLWISDCNGALNWMPLITPN
                     DDIPLGENIGLLGFPLGFELGINVNYTQGIVNSLRTRNSIPVLQIDAGATHGSSGGPV
                     FRRSDGKVVGMVQAIL"
BASE COUNT          327 a          328 c          364 g          296 t
ORIGIN      
        1 caggatggcc tgtaccattc caaccacttt cccatctgac cgtcgaaaaa ccgggcctcc
       61 ggaagaacca tgggtggcac cggcatcaat ttgcagaacc gggatgctgt ttcgggttcg
      121 caggctgttg acgatccctt gcgtgtaatt gacattgatg ccaagttcga aaccgagtgg
      181 gaaccccaac agcccgatat tttcaccaag cggaatatca tcgttgggcg taatgagcgg
      241 catccagttg agcgcaccat tgcagtcaga aatccacagt aaggccagat catgctgggc
      301 acttccggcg acatagagca gtggtcgcag atagttggtg ccactcaggc aaatatccat
      361 gcgtttggca tcagatacca cgtggtaaca ggtcaggata aacccacctg ggttgatcac
      421 aaaccccgtt cctgacgcat gctgaccgtc ctggcattca accagaatga acccaaccgc
      481 accggtcagt ccgccgggta cggttggcga ctgatttcca ggaaagctgg ctggcatata
      541 gaccggtttt gggggaggga gtttcccagg aagcaaaatc ggctggtctt cgagtggtgg
      601 ttggggtgcc ggtgccggct gtgccgcccc ggcgggttga ccataagctt gaggtggtgg
      661 cggtggtgga gcgaccggag cgaccagacc cgctggcacg ccaaaatggt caaacaaggc
      721 cgactgcccg gctcgaaatc cgctgctcac cagacccagt cgccagccat ctttctgata
      781 cagccgacct aaaaccagcg ccttttcacc atcaaaatcc tgggctgaaa actgatagcg
      841 ggcaacttcc tgtgatccgg cagataccac caggctcccg ctctgaatgt cgctggcatt
      901 ttccagcagg cagcctgtcc gttcccctaa cgacaacgcc agcacaacct cttttgcctc
      961 gcctgggagc gaactcagat tgacgttgat tgaagtctgg gtgggtgtgg tcgaagcaat
     1021 ttgaattgac tggcacggac ttttgggttg attggaaaac actaaatatt cggacttgag
     1081 caacttttgc tgctcattga gggcaaggca ataaaactgg gcatggtctt ttaaaccgga
     1141 taaggcaata tcaaggcgga tttgtgattg tggagttaaa tcagagagct taatgcgctg
     1201 gcctttcaca agttgcttgc tcataacggt ttcctgttct gaatgaagaa ttgagaattg
     1261 agaatgaaga attgagaatt gagaattgag aatgaagaat tgagaattga gaatg
//
LOCUS       DAOQDO010002160         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126078_length_1315_cov_2.768254, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002160 DAOQDO010000000
VERSION     DAOQDO010002160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126078_length_1315_cov_2.768254"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..687
                     /locus_tag="PKZ53_25720"
     CDS             40..687
                     /locus_tag="PKZ53_25720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43906.1"
                     /translation="MNYQTDEFVRHLNLTLAWCLTRVNFDDPKNCLRSRELRPGLEAF
                     GYGDDDIWIHPQLVDQVLRQRQYHFSDSGISNQFQLITNIPGRLLLVAQDYSNHNNLT
                     SDITGGFFDYNDVPPWDTWVMLIEGNREGMTIGDWESDWPPTLSFSLGDDSMMCWLLV
                     TWVPEVFCSMVQEAIEAEAIGMLAWADNLPEKQSGAQPNFRQVVPLWLQNLMNPK"
     gene            751..1077
                     /locus_tag="PKZ53_25725"
     CDS             751..1077
                     /locus_tag="PKZ53_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNJ43907.1"
                     /translation="MGWVTTALKELKAGNTVQIRPQGGSMRGRIESGQLVTLKPVTGT
                     EVAVGDVVLVRWKGNYLLHLVKAIEAGQVLIGNNLGKENGWVSVNDIVARVVESDLQT
                     NHSESE"
BASE COUNT          371 a          278 c          323 g          343 t
ORIGIN      
        1 cacttacaat cgtgatttcc ttatcaccga taatgtctaa tgaattatca aactgacgaa
       61 tttgttcgcc acctcaattt gacgcttgcc tggtgtttga cacgagtcaa ttttgacgat
      121 cctaaaaact gtttgcgctc acgtgaactt cggccaggcc ttgaggcatt tggctatgga
      181 gatgacgata tttggataca tccccaactg gttgatcagg tgcttcgcca acggcaatac
      241 catttttctg attcaggcat ctccaaccag tttcagttga ttaccaatat accagggcga
      301 cttctgctcg ttgcgcagga ttattccaat cataacaatt tgacttcgga tatcacgggc
      361 gggttttttg attacaacga tgttccaccc tgggatacct gggttatgtt gatcgaagga
      421 aatcgcgaag gaatgacaat cggggattgg gaaagtgatt ggccgccaac tttgtccttt
      481 tcgctggggg atgacagtat gatgtgctgg ctactggtga cctgggttcc agaggtgttt
      541 tgttcaatgg ttcaggaagc gattgaagcc gaggcgattg gcatgctggc ctgggcagac
      601 aatttacccg aaaaacaaag tggcgcgcag ccaaactttc gtcaggttgt tccactgtgg
      661 ctccaaaatc tgatgaaccc aaagtagcct cgtgatactt cgagggccta acagccattc
      721 tcattttcag acggaaaggc aatcaaacca atgggttggg ttacaactgc actcaaagaa
      781 ctcaaagctg gaaacacggt tcaaatccgt ccgcagggtg gttccatgcg gggccgaatt
      841 gaaagcggcc agctcgttac gctcaaacca gtcacaggaa cagaagtcgc ggtcggtgat
      901 gtagtgttgg ttcgctggaa aggcaattat ctcctgcacc tggtcaaagc cattgaggct
      961 ggacaggtgc tcatcggaaa taatctaggc aaggaaaacg gatgggtctc tgtcaatgat
     1021 attgtggcga gggtagtgga gtcagacctt caaacaaacc attcggaatc tgaatgaata
     1081 tcaatttgga taagttatga atatcaggtg gaatgaaatt ttatcaaaca aggtcttctg
     1141 gtcgggctac tatgctcaat cagaagatac tcttgaatac ccattaatgt ggcttcagca
     1201 cttcgggtta gatcaaacat caaacgttga ccaggaagag ttaacccgaa tattgcatga
     1261 aaaaaaaggc aaaatcagta taatcctttg aaaataagca agatagaaag gtcaa
//
LOCUS       DAOQDO010002161         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126249_length_1314_cov_3.461477, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002161 DAOQDO010000000
VERSION     DAOQDO010002161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126249_length_1314_cov_3.461477"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PKZ53_25730"
     CDS             complement(<1..132)
                     /locus_tag="PKZ53_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ43908.1"
                     /translation="MLKVGVIGVGFLGRHHARIYASLPDVTLVGVCDTREQAGKEIAA
                     "
     gene            complement(246..>1314)
                     /locus_tag="PKZ53_25735"
     CDS             complement(246..>1314)
                     /locus_tag="PKZ53_25735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43909.1"
                     /translation="GLFLKIFLPSVLGFVIILGGVVGYIVKTVTHPPKSAVDEEAPAQ
                     LTKVMSMYWEKKTFSSDGTQFNYWVLVFGGGAPGIVLTHGYGMNREDMLLLGYRLWER
                     GFNVMMYDLRYHGNNKAEFSGLGALERADLQNALKEFKGLKYNKDIGKISPAGIPLVD
                     PDNIGVLGIDIGAYASLMVAADDDSVKGIVIDSPPLSPSDFTHNRLGKVFNLKSDLAY
                     SLIDSGMDIYIKGKYNNAGSAIAAAQKIKTKGKKVLVISGGDNPIAKVSSKKIFDTIG
                     LEHAQISTPAVTKFSSFTKETGDEYMGNVGDFFRNVVFLPPPPPPDGAQPPATGTTPT
                     PAGTAPAPASPTPAPTTPAKK"
BASE COUNT          340 a          322 c          314 g          338 t
ORIGIN      
        1 ggctgcgatt tcttttccag cttgttcgcg ggtatcgcaa acaccgacca gtgtgacatc
       61 gggcagcgag gcataaattc gggcgtggtg tctgcctaaa aaaccaacgc cgatgacacc
      121 gactttaagc ataagttcac tgaaggattg aaaagaacca cctgccagcg caggtggttt
      181 ttgaccggat acagggcttt agaaaaaagg gtaccgtgac tcagagccga aaatcagact
      241 ggtttttatt tcttggctgg ggtggtcggt gccggtgttg ggctggccgg tgctggtgcc
      301 gttcctgccg gtgtgggggt tgtccccgtc gccggaggtt gagccccatc tggaggagga
      361 ggtggtggca aaaacaccac attgcggaaa aagtccccga cattgcccat atattcgtca
      421 ccggtttctt tggtaaacga agagaacttg gtcacggctg gggtggagat ttgagcgtgc
      481 tccaacccaa tcgtgtcaaa aatctttttg gacgatactt tggctatcgg gttatcacca
      541 ccagaaatca ccaaaacctt tttgcctttg gtttttatct tctgtgcggc agcaatggct
      601 gatccggcat tgttgtactt tcctttgata tagatatcca tcccggaatc aatcaatgag
      661 taagccaaat cagatttcaa attaaagact ttacccaatc ggttgtgggt aaaatcagat
      721 ggggagagtg gaggagaatc aatcacaatt cccttaacgg aatcatcatc ggctgccacc
      781 atcagtgagg cataggcacc gatatcaatg ccaagtactc cgatattgtc tggatcaacc
      841 agagggatgc ctgccgggct gattttaccg atatccttgt tgtatttgag ccctttgaat
      901 tctttcaggg cattttgcag gtcggctctt tccagggcac caaggccaga aaattcagct
      961 ttgttgtttc cgtgataccg caaatcatac atcatcacat taaagccacg ttcccacagt
     1021 cggtatccca gcaagagcat gtcttcgcga ttcatcccat acccatgggt cagcacaatt
     1081 ccgggtgccc ccccaccaaa gaccagaacc cagtagttaa actgagttcc atcagaagaa
     1141 aaggtcttct tttcccaata cattgacatc actttggtca attgggctgg cgcttcttca
     1201 tcaactgcgc ttttcggtgg atgagtaacg gttttgacga tatatccaac cacaccaccc
     1261 agaataatca caaatcccaa aacagacggc agaaagatct ttaaaaataa cccc
//
LOCUS       DAOQDO010002162         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126255_length_1314_cov_3.194599, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002162 DAOQDO010000000
VERSION     DAOQDO010002162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126255_length_1314_cov_3.194599"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..771)
                     /locus_tag="PKZ53_25740"
     CDS             complement(52..771)
                     /locus_tag="PKZ53_25740"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNJ43910.1"
                     /translation="MYEWWKRIVLALLKVEDKKPQPLPGHREGDFLEIVRACPAFWDF
                     QLLTWRIYLISMVAIATFLSCIFLLVNPLTLLVIIPVWILLAIKAALIYMTMRLDYEM
                     RWYVITDRSVLIREGAMDVREICLTFANAQNVFVRQGPVERWFGFSNVEINTAGGSAG
                     PHQGGVQSHQAVLRGVSNAEHLRDLILLKLKQHRTAGLGDPDDHEHHDQKHPALNPVL
                     VKEILDEARQLRKVLETSNLS"
     gene            complement(764..>1314)
                     /locus_tag="PKZ53_25745"
     CDS             complement(764..>1314)
                     /locus_tag="PKZ53_25745"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNJ43911.1"
                     /translation="RILPMKHDDDAHLIEVDSKTVFAITRPHDNLFYLYFIYSLLSNI
                     VFPIVFLPLYFRFKTLRYRFDNEGIAISHGILWRQETYLTYSRIQDIHVSRNIFERWL
                     GLGTVQIQTASGSSSAEEAIVGVCQYNEIRNFLYSRMRGQKVPSAAQPQLAVEQSVDD
                     MLAGIRDELRAIRELVEGKRHV"
BASE COUNT          342 a          332 c          320 g          320 t
ORIGIN      
        1 ggtctttcgg catctccttt ttcatatgtc ctccacaggg tgacgttggg ctcaggaaag
       61 gttcgaagtt tccaatacct ttcgtaattg acgggcttcg tccaggattt ctttgacgag
      121 aaccgggttc aacgccgggt gcttctggtc gtgatgctca tgatcatcgg gatctcccaa
      181 acctgccgtt cgatgttgct ttaacttcag gaggattaaa tcccgcagat gctcagcgtt
      241 gctgacaccg cgcaacacgg cctggtgaga ctgaacgcct ccctgatggg ggccagcact
      301 cccgccagcg gtattaattt cgacgtttga aaacccaaac cagcgttcca ctggtccttg
      361 cctgacaaac acattctggg cattggcaaa ggtcaggcaa atttcccgga catccattgc
      421 gccttcgcga atcaacacgc tccgatcagt aatgacgtac cagcgcattt cataatccag
      481 ccgcatggtc atataaatca gagcggcttt gatggccagc agaatccaga ccgggataat
      541 caccaaaaga gtcagtgggt taaccagaag aaatatacaa cttaagaatg tcgcaattgc
      601 aaccattgag atcagataga tccgccaggt cagcaactgg aaatcccaaa acgccggaca
      661 ggcacgcaca atttcaagga aatcaccttc ccggtgccca ggcaatggct gtggtttttt
      721 atcttcgact ttcaataagg ccaggacgat gcgtttccac cattcataca tgacgttttc
      781 cctccaccag ttcccggatg gcccgaagtt catcccgaat tcctgccagc atgtcatcca
      841 ctgattgttc gacagcaagc tgaggttgag cggcggaggg tactttttgt cctcgcattc
      901 tggagtagag gaaatttcgg atttcattgt actgacaaac gcccacaatc gcctcttcag
      961 cactggaact cccagaggct gtctggattt gcacagttcc caaccctagc cagcgttcaa
     1021 aaatgtttcg ggaaacgtga atgtcctgaa tgcgtgagta cgtcagatag gtttcttgcc
     1081 gccacaagat gccgtgcgaa atggcaattc cttcattatc aaagcgatac cggagcgttt
     1141 taaatcgaaa atagagcggc agaaaaacga tggggaaaac aatattggac agcaggctat
     1201 agataaaata gagataaaag aggttgtcgt gagggcgggt gatggcaaag accgttttgg
     1261 aatccacctc gattaagtgg gcgtcatcat cgtgtttcat gggcaggatc cttt
//
LOCUS       DAOQDO010002163         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126477_length_1313_cov_2.861685, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002163 DAOQDO010000000
VERSION     DAOQDO010002163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126477_length_1313_cov_2.861685"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PKZ53_25750"
     CDS             <1..1246
                     /locus_tag="PKZ53_25750"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43912.1"
                     /translation="NLVPEVGGEEGASFEKVSPTQLKQLVDLNLNYGHLLISFGQLED
                     AEKKLQLALALTRHLNDELLRGRTLGALGELCEARSEYALSLLYCRQALEILRRGGDT
                     KAESRTLCTIGMVYDRLGQLARSAESQEKSLALSRSIKDRMGESMALRELAYVLIKQG
                     QYRRAVECAQSASEIAQQMNDRLGYSISLNILGVAYLAQNRPDRAHELFQESLKAAAE
                     LNRPRGEAIELANLGECQRVLGDFGKSMDLNQQALALSRQIGSKQLEATALFFLGRTY
                     QSRHEHEQALDYFQQAAKLISSISDRILECDVLLGIVEIYLSQSRPSDAMESIQSVLT
                     TTADFRALPWRWRALFVQGRCQFFLGQTKEAQQSLSEAVEIVKQLGNQLSSDVPQSVY
                     FEDKKPLSRALESVLIAVKNLG"
BASE COUNT          328 a          318 c          357 g          310 t
ORIGIN      
        1 caatctggtc cccgaagttg gcggtgagga aggcgcttcg tttgaaaagg tctcacccac
       61 gcaactcaag caactggtgg acctcaattt aaattatggc catttgctca ttagttttgg
      121 tcagcttgaa gacgccgaga agaaattgca gcttgctctg gccttaaccc gtcatttaaa
      181 cgatgagttg ctgcgtggtc ggaccttagg ggcgctcggg gaattgtgcg aagcccgaag
      241 tgaatatgct ctgtcattgc tttattgccg gcaggccctg gaaattctcc gacgcggcgg
      301 ggacaccaaa gctgagtccc gaaccctgtg cacaattggc atggtctatg atcggttggg
      361 gcaactggcc agatcggctg aatcacagga aaagagcctg gcgttgtcac gttcaatcaa
      421 agatcgaatg ggcgagagca tggccttgcg cgaactggcg tatgttttga tcaaacaagg
      481 tcagtatcgc cgggccgttg agtgtgccca gtctgccagc gagattgccc agcagatgaa
      541 cgaccggcta ggatactcaa tttccctcaa tattcttggc gttgcctatc tggctcaaaa
      601 tcgcccggac cgcgcccacg agttattcca ggaatcactc aaggcagcgg ctgaactcaa
      661 tcgcccacgc ggagaggcca tcgaactggc aaatctgggt gaatgtcagc gggttttggg
      721 tgattttggc aaatctatgg atctcaacca gcaggcactg gccctgtcgc gtcaaatcgg
      781 cagtaaacag cttgaggcaa cggcgttgtt ttttcttggc cgaacctacc agagccgcca
      841 cgaacacgaa caggcactcg attattttca acaggcggca aaactcattt ccagcatcag
      901 cgaccgaatt ctggaatgtg atgtgttgct tggaattgtt gaaatctatc tttcacaaag
      961 tcgcccgtcc gatgccatgg agagtattca gagcgtgctg acgacgacgg ctgactttag
     1021 agccctcccc tggcgctggc gggcattgtt tgtccagggg cgatgtcagt ttttccttgg
     1081 ccaaaccaaa gaagcccagc agtcgctgag cgaagccgtt gaaatcgtca aacagcttgg
     1141 taatcagttg agttcagatg tgccccaaag cgtttacttc gaagacaaaa agccgctctc
     1201 ccgggcgctt gaatctgtct tgattgctgt caaaaatttg ggataagcct gtaacttctg
     1261 aagaatgaag aattgagaat gaagaatgaa gaatgaagaa ttgagaatga aga
//
LOCUS       DAOQDO010002164         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126499_length_1313_cov_2.444356, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002164 DAOQDO010000000
VERSION     DAOQDO010002164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126499_length_1313_cov_2.444356"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..484)
                     /locus_tag="PKZ53_25755"
     CDS             complement(62..484)
                     /locus_tag="PKZ53_25755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ43913.1"
                     /translation="MDNLPHDKPENEVFLQIPMLPNMELAATETAHRIAEFMQFDPNK
                     IDEIKISLIEGILNAFEHSQSKDKMVYIKFLMRDDELEVTIQDYGIGFSISNVNTKIE
                     PLQYRGYGLTLISNLMDRVDVTSGPDGTKVVMSKRRHS"
     gene            complement(670..>1313)
                     /locus_tag="PKZ53_25760"
     CDS             complement(670..>1313)
                     /locus_tag="PKZ53_25760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43914.1"
                     /translation="LHFGLSSNVSFSVEGVMRNFLNIHETGPSPIPLSIAPGATSTSD
                     FGDLTLSTKVKLRNEGKRMPSLGFKLGVTLPNSDQARGIGTNTLNLFATTLVGKNFLN
                     GRLRTFGNIGMGILADTLDARAQQDVLLYGVAGILKVNERLNLLGEVNGRYNPRRAKP
                     GLESQSQARFGMQLKAGGFQWDVAAIKGLTDFSPQSGVSLGVTYDFKAFEPVK"
BASE COUNT          322 a          304 c          335 g          352 t
ORIGIN      
        1 cttgtcgatg ttaaaacctg aacaattggc cagtgagtaa ctttcagcgt ggaactcaga
       61 atcaggaatg tcgccgtttg gacatcacca ctttggtgcc gtctggacca gatgtgacat
      121 caacccggtc catcaaatta ctaatcagtg tcagtccata tccacgatat tggagcggtt
      181 caattttggt gttgacgttg gaaatgctga acccaatgcc atagtcctga atcgtgactt
      241 caagctcatc atcccgcatt aaaaacttga tatagaccat cttgtctttg ctctgactgt
      301 gttcaaaggc attgaggata ccttcaatca gagaaatctt gatctcatca attttgttgg
      361 gatcaaattg cataaattca gcaatccgat gggctgtttc agtggctgcc agttccatat
      421 tgggcagcat cgggatttga agaaagactt cgttttctgg cttgtcgtgg ggcaggttat
      481 ccattgaatg acactccgct gtaaattcta cacaaaagtg atgcctggcc aggtagaccg
      541 cgctatggta gcacagttgg aattttgtat tttgaagaat tcggatggtg gatcgggctc
      601 aagggatcgg gctcaagggt gagttttagc catccgtccc gatgtctttc gcccaggccc
      661 taatgcaggt tatttcactg gttcaaaggc tttgaagtcg taggtcacac ccagagatac
      721 accactctgt gggctaaagt cggtcagtcc cttaatcgca gctacatccc attggaaccc
      781 gccagctttg agttgcatcc caaagcgggc ttgagattgg ctttcgagtc ctggttttgc
      841 ccgacgcggg ttgtaccgtc cgttgacctc acccagcagg ttcagtcgtt cattgacctt
      901 gagaataccg gcaaccccat acaggagaac atcttgctgg gcccgggcat caagcgtgtc
      961 ggccagaatc cccattccga tgttaccaaa agttcggagc cgtccgttga gaaaattttt
     1021 accaaccagt gtggtggcaa acagattgag ggtattggtg ccgatgccgc gagcctgatc
     1081 agaatttggg agggtcacgc caagtttgaa tccgaggctc ggcatacgct ttccttcatt
     1141 tcgaagttta acttttgttg acagggtaag gtcaccaaaa tcactggttg aggtggcgcc
     1201 gggcgcaatg gagagtggga tcggtgatgg gccagtttca tgaatattga gaaagtttcg
     1261 catcacgcct tcaactgaaa acgagacatt ggatgacagg ccaaagtgta acc
//
LOCUS       DAOQDO010002165         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126704_length_1312_cov_2.248210, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002165 DAOQDO010000000
VERSION     DAOQDO010002165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126704_length_1312_cov_2.248210"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PKZ53_25765"
     CDS             <1..1261
                     /locus_tag="PKZ53_25765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ43915.1"
                     /translation="GNVVSQSRNVFPQFAPVNFGAGVVFADLLAGNPSFITFGEDGSV
                     PLVAPGTLNTFGFGRELVAESIGQLLGVRGFGLGFTLPEKNFRTPYVHQYSVSFEQAL
                     ADRYVVSVGYVGTKGRKLIRFRTPNGGSFTPLQLGVLGDDSDISPLFGTSDSATRKRP
                     EDRLGPFTVFEGSAESEYHALQASLIRRFADGLGFQLAYTYGHAIDDVSDVFDMSGAY
                     ILAQDELGGAAGLRNERGSANFDVRHRFTAGWQYELPFWKQHRLLGGFQLSGIVTLQT
                     GQPFTVNSAVDANLDGNLTDRLNTLQGLTIRDEGQTRILLAPGVSTTSLLAIDGAQPL
                     FNLLNGSVGRNTFRAAGVASVDLALDKRFRIGENNAFSIRIEAYNLFNRTHFGIPVRI
                     LEAPSFGSSVNTSVSPRTIQFALKYTF"
BASE COUNT          285 a          309 c          371 g          347 t
ORIGIN      
        1 ggggaatgtt gtcagtcagt cgcgcaatgt gtttccacaa tttgccccag tgaatttcgg
       61 tgcgggcgtg gtatttgctg accttctggc tggtaacccc tcctttatta cttttggtga
      121 agacggctca gtaccactgg tggcaccagg aactctcaat acatttgggt ttggtcgtga
      181 attagtggct gagagtatcg gccagttact tggtgttcgc ggctttgggc tcgggtttac
      241 attgccggag aaaaatttcc gaacgcccta tgttcaccag tacagtgtat cgtttgagca
      301 ggcgctggct gatcgctatg tggtctcggt tggatatgtg ggcaccaaag gccgaaaatt
      361 gattcgcttc cgcactccga atggtgggtc gttcactcca cttcaattgg gggtgttggg
      421 tgatgactct gatatttccc cactatttgg caccagtgat agcgcaacac gaaagcgccc
      481 agaagaccga cttgggccct ttacggtgtt tgaaggttcg gctgagtcgg aatatcacgc
      541 cctgcaagcc tcattgatcc gccggtttgc tgatgggctt ggttttcaac tggcctatac
      601 ctatggccac gcgattgacg atgtttcaga tgtgtttgat atgtcgggcg cttacattct
      661 ggcccaggat gaacttggtg gggctgccgg gcttcgcaat gaacggggga gtgccaattt
      721 tgacgttcgc caccggttta cggcgggctg gcaatacgaa ttgccattct ggaaacaaca
      781 tcgcctgctt ggagggttcc agttgtcagg gattgtgacc ctccaaaccg gtcagccctt
      841 tacggtgaac agtgccgtgg atgccaacct ggacgggaat ttgaccgacc ggttaaatac
      901 gctgcaagga ctgaccattc gggatgaagg ccagacccgc attctgctgg caccaggcgt
      961 cagcaccacg tcgctcctgg cgattgatgg tgctcagcca ctgtttaacc tgttgaatgg
     1021 cagtgttggt cgcaatacgt tccgggcggc gggcgtggcc agcgttgatc tggcgctcga
     1081 taaacgattt agaattggtg aaaataacgc gttctcaatc cgaatcgaag cctataacct
     1141 gtttaatcgc acgcactttg ggattccagt ccggattctt gaagctccgt cgtttgggtc
     1201 ttcggtcaat acttcggtct caccgcgaac gatccagttt gcactgaaat acacctttta
     1261 gaatgaagaa tgaagaatga agaatgaaga atgaagaatg aagaatgaag aa
//
LOCUS       DAOQDO010002166         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126886_length_1311_cov_2.795382, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002166 DAOQDO010000000
VERSION     DAOQDO010002166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126886_length_1311_cov_2.795382"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1214
                     /locus_tag="PKZ53_25770"
     CDS             90..1214
                     /locus_tag="PKZ53_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ43916.1"
                     /translation="MRRLSWRNLIHDKVRFGVTLTGVVFAVILVAIQVGLLVGFSRAT
                     SDIIHHSGADLWIRSKDVPYIEVGVVFSERKLYQARAVPGVQWAEKFILQFIDWKRPD
                     GSNEGGMVLGFNPDRPLGGPWNLTAGQIEDLKADDTIIIDELYQEKLGVTHLGQVVEI
                     NNRRAKVVGFTRGIRTFTTSPAIFTSFKNALNYANFREDQTQYILVKVAPGYDVEQVR
                     RELGLRIKDVDISTAAEFGQMTRDYWLFSTGAGVTVIVAAILGLIVGVVVVAQTIYAS
                     TVDHLKEYGTLKAMGADNSYIYRVIIEQAVISAVIGYVLGISVSLFVVQVSQSGVTAI
                     FMPWQVGLGLFVLTLMMCIGASIVSIKKVTQIDPAMVFKG"
BASE COUNT          294 a          309 c          360 g          348 t
ORIGIN      
        1 ggtcaacggc ttaaaagcct gatttttttg ccttcttgct taacactgtt aagcatcttt
       61 tttgcgttaa acgaacgagg tacttccgaa tgagacggct ctcctggcgc aacttaatcc
      121 acgataaagt tcggtttggc gtgactctga cgggtgtggt gtttgccgtg attctggtgg
      181 cgattcaagt tgggctgctg gttgggttta gccgggccac ctcagacatc atccatcatt
      241 cgggagctga cctttggatt cgatccaagg atgttccgta tatcgaagtc ggggttgtgt
      301 ttagcgaacg caaactctat caggcacgtg cggtgccagg tgttcagtgg gctgagaaat
      361 tcattctgca gtttattgat tggaaacgtc cggatggttc aaatgaaggc gggatggtgc
      421 ttggattcaa cccggatcgt ccacttggcg gcccgtggaa tctcaccgcc ggccaaatcg
      481 aagacctgaa agcggatgac accatcatca ttgacgaact ctatcaagaa aaactgggag
      541 taactcatct cggccaggtg gttgaaatca ataaccgtcg ggccaaagtg gttgggttta
      601 cgcgagggat tcggaccttt accacatcgc cggccatttt tacgtcattt aaaaacgcgc
      661 tcaattacgc caattttcgc gaagaccaga cgcagtacat tctggtcaaa gtcgcacccg
      721 gatatgacgt cgagcaggtt cggcgtgaac tcgggttgcg gatcaaagac gtggatattt
      781 caacggctgc cgaatttggc caaatgaccc gtgattactg gctatttagc accggtgctg
      841 gggtcaccgt gattgtggcg gccattttgg gattgattgt cggcgtggtg gtggtcgctc
      901 agacgattta tgcctcaacc gttgatcacc tcaaagaata cggcaccctc aaggccatgg
      961 gcgccgacaa cagctatatc tatcgagtca ttattgaaca ggctgtcatc agtgccgtca
     1021 ttggatatgt gcttggaatt tcagtcagtt tgttcgtcgt ccaggtcagt caaagcggag
     1081 tcaccgcgat ttttatgccc tggcaggtgg gcctcggtct gtttgtcctc acgctaatga
     1141 tgtgcattgg agcttcaatt gtttccatta aaaaagtcac ccagattgac ccggcaatgg
     1201 tctttaaggg ctgaaaaacc agggcggaag acatcgggct tggggctgaa gactccgggc
     1261 tcagggtatt gaacccgttt tcttcagccc cgagcttgcg agtcttcagc c
//
LOCUS       DAOQDO010002167         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126889_length_1311_cov_2.742834, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002167 DAOQDO010000000
VERSION     DAOQDO010002167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126889_length_1311_cov_2.742834"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1241)
                     /locus_tag="PKZ53_25775"
     CDS             complement(114..1241)
                     /locus_tag="PKZ53_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNJ43917.1"
                     /translation="MAQEFTLGIEEEFQIIDPNTRELRSRVAEILEEGMMLLGEQVKQ
                     EMHQSQIEVGTGICKNIQEARADVTKLRRTIATLAAKKNLAIVAASTHPFSHWKDQLI
                     TPDERYYALIEEMQQVARALSIYGLHVHVGMESREDAIHIMNAARYFLPHVLALSTSS
                     PFWIGRNTGLKSYRSEVFKAFPRTDIPDYFGSASEFDNYVKLLVKTKCIDNGKKIWWD
                     VRPHPIFPTLEFRTCDLPAKVDEVIAIAALFQAIVAKLYKLLKQNMGFRLYRRALIQE
                     NKWRAVRYGLDGKLIDFGKQIEVPVRDLICELLEFVDDVVDELGSRKEIEYVHTIMQN
                     GSSADRQLRVYEETGDLHAVVDNLIKETTEGVLPVAPVAKK"
BASE COUNT          290 a          319 c          328 g          374 t
ORIGIN      
        1 tgacaaggta cttaacagat tttcttcatt cttcattctt cattcttcat tctcttcatt
       61 cttcattctt cattctcttc attcttcatt cttcattctt cattcgagag gttttacttc
      121 ttggcgaccg gggcgacagg caggacccct tcggtcgttt ctttgatcag gttgtcaacc
      181 acagcgtgga ggtcgccggt ttcttcataa accctcagtt ggcggtcggc gcttgatcca
      241 ttttgcataa tggtgtggac atattcgatt tccttgcggc tgccgagttc atccaccaca
      301 tcatccacaa actccaacaa ttcacagatt aaatcccgga ctggaacttc aatctgcttt
      361 ccaaagtcaa tcagctttcc gtcaagtcca tagcggacag cacgccattt attttcctga
      421 atgagcgcac ggcgatagag acgaaatccc atattctgct tcaacagttt atagagtttg
      481 gccacaatcg cctggaacaa ggcggcaatc gcgatgactt catcaacttt tgctggcagg
      541 tcacaggtgc ggaattcgag cgtcgggaag attggatggg ggcggacatc ccaccagatc
      601 ttcttgccat tgtcgataca tttggttttg accagcagtt tgacatagtt gtcaaattca
      661 ctggctgatc caaagtagtc tggaatgtca gtccgtggga aggctttaaa gacttctgag
      721 cggtatgatt tcagaccggt attgcgacca atccagaacg gggaactggt cgagagcgcc
      781 agcacgtgcg gcaagaaata ccgggcggcg ttcataatat gaatggcatc ttcgcggctt
      841 tccatgccga cgtgaacgtg cagcccatag attgaaagtg cacgggcaac ctgctgcatt
      901 tcttcaatta aggcgtagta gcgttcatcg ggtgtaatca actggtcttt ccagtgtgag
      961 aacgggtggg ttgaggccgc gacaatcgcc agatttttct tggcggccag ggtggcaatc
     1021 gtccgtcgaa gtttggtgac gtcagcccgg gcttcctgga tgtttttgca aatgcccgtt
     1081 ccgacttcaa tctgagactg gtgcatttcc tgtttgacct gctcgccgag gagcatcatg
     1141 ccctcttcca gaatctcggc cacgcgtgag cgaagttcgc gggtgttggg gtcaataatc
     1201 tgaaattctt cttcaatgcc aagtgtaaat tcctgtgcca tgtgggtatc cccttcattg
     1261 agggtcgtgg gattggtgat cgagagtgag gttagtcagt ggattaaaca c
//
LOCUS       DAOQDO010002168         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126945_length_1311_cov_2.079618, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002168 DAOQDO010000000
VERSION     DAOQDO010002168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126945_length_1311_cov_2.079618"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..>1311)
                     /locus_tag="PKZ53_25780"
     CDS             complement(444..>1311)
                     /locus_tag="PKZ53_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNJ43918.1"
                     /translation="SPQDLGIWKDEHLPALQRVTEFVKAHGAAAGIQLAHAGRKASTA
                     QPWRGGQPVTPDNGGWGDIVGPSAIPFSDTYPTPRELSIDEIHALTGQFEAAAHRALA
                     AGFQVVEIHAAHGYLIQEFLSPISNHREDEYGGSFDNRTRLLCEIAVRIRNVWPQSLP
                     VFVRISATDWVENGWDLAQSIELAAKLKTIGIDLIDCSSGGNVARAPIPVGPGFQTPF
                     AAAIRREANLPTGAVGMITSAVQADHIIRTGQADVVLLARELLRDPYWPLHAARELNQ
                     PIQWPVQYERAK"
BASE COUNT          290 a          382 c          362 g          277 t
ORIGIN      
        1 gcctgcgaag caggtcacag agagtagccc ccggtgcaac cgggggaatc gaatgccgcc
       61 gcattccagc ctgcgcagca ggtgacagag agaaacgagc acctccaggt cacgtgcctt
      121 caccggtatt ccgccgattg ccgtgaactt ccttggacac tctctgccgc ctgctgcgca
      181 ggctggaaag atgaccgacc gatcccccgg ttgcaccggg ggctattgtc taccacccgc
      241 ttcgcaggct aaaactgaat ttccaattca tcgaacccat cacgggaaaa attacagaaa
      301 gaactccagc gatcatgctc acctgagttt cccgacttaa agccttctat ttcagataat
      361 taaatggctg tcaacgcgtt gacacttctg aaatggattt gaacttcccg ctcttttgga
      421 ttgaccactc gctcttcacg ctttcacttc gctcgctcat attgaaccgg ccattggatt
      481 ggctggttga gttcgcgggc ggcgtgaagt ggccagtacg gatcgcgcag cagttcccgc
      541 gccagcaaga ccacatcagc ctgaccggtg cggataatgt ggtcagcctg caccgccgaa
      601 gtaatcatcc ccacggcacc ggttgggagg ttcgcttcgc gccggatggc tgcggcaaac
      661 ggtgtctgga aacctggtcc aaccgggatt ggagcccggg cgacgttgcc tcctgacgaa
      721 caatcaatca aatcaatgcc gatggttttc agtttggccg ccagttcaat ggattgcgcc
      781 agatcccagc cattttcaac ccagtcggtc gcggaaatcc gaacaaacac cgggagcgat
      841 tgcggccaga cattacgaat ccggacggca atttcgcaga gcaaacgggt tcgattgtca
      901 aaacttccgc catattcatc ttcccgatga ttggaaatcg gagacaaaaa ctcctgaatc
      961 aggtaaccgt gcgccgcgtg gatttcgacg acttgaaatc cagccgcgag tgcccgatga
     1021 gccgccgctt caaactggcc ggtcagggcg tggatttcat caattgagag ttcgcggggt
     1081 gtcggatatg tgtcagaaaa cggaatcgcg ctgggaccga cgatgtcacc ccatccgcca
     1141 ttatcgggag taaccggctg gcctccgcgc cagggttggg cggtgctggc tttgcgtccg
     1201 gcgtgggcaa gctggatgcc ggctgccgca ccgtgagctt tgacaaattc ggtcactcgt
     1261 tgcagtgccg ggagatgctc gtccttccag atacccaaat cctgcggaga a
//
LOCUS       DAOQDO010002169         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_126952_length_1311_cov_2.023885, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002169 DAOQDO010000000
VERSION     DAOQDO010002169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126952_length_1311_cov_2.023885"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PKZ53_25785"
     CDS             complement(<1..1170)
                     /locus_tag="PKZ53_25785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43919.1"
                     /translation="MKRFLQNVQLWPFTGQTVVRMIVTMLLVMGSAWLWFGPAAPLPA
                     TPSPALKPDTSRLPHQSAPPDRQAGESDPPSVTAHSYQATRPVAGPTTSGPQPVDKLP
                     LRFETNLGQTDPSVQFLARGQGYAFFVTATENILVVESNESPSTAQTVKLEFAGAQPP
                     TQILGEEKLPGVKHYLKGSQPAEWQTHVPTYERVRARDVYPGIDVVYYGHHSELEFDL
                     ILQPGAAPSQPQLHFRGAKRLSLNADGELVIALPHGGELRQPKPVAYQEINGQREAIA
                     VAYVLKNSETVGFQMAEYDPSQPLVIDPILLYASYLGGTEADRGYRVTVGNDGYLYMA
                     GMTRSLNFPTANGTQSSSGGDLDFFLSKLSADGSTLLYSTYLGGSGTEVCYGLTAS"
BASE COUNT          292 a          357 c          366 g          296 t
ORIGIN      
        1 actggcagtc agtccataac acacttccgt gccgctgccg cccagatagg ttgaatacag
       61 cagggttgag ccatcggcgg acaatttgct caggaaaaag tccagatcgc cacccgagga
      121 cgactgcgtt ccattcgccg ttggaaagtt caacgagcgg gtcatacccg ccatatacaa
      181 atagccatca ttccccacag tcactcgata tccacggtcg gcctcggttc cgcccaaata
      241 actggcatac agcagaattg ggtcaatcac cagcggttgc gaaggatcat actctgccat
      301 ctgaaatccg acggtctcgg agtttttcag tacatacgca accgcgatag cttcacgctg
      361 tccattgatc tcctgataag ccaccggttt gggttgtcgc agctctccgc cgtgaggcag
      421 ggcgatgacc agttcaccgt cagcgtttaa agacagccgt ttggcaccac ggaaatgtaa
      481 ttggggctgt gacggtgctg ctccaggttg aaggatcaga tcaaattcca gttcggagtg
      541 atggccgtaa tacaccacat caatgcccgg atagacgtcg cgcgcgcgga cccgttcata
      601 ggtgggaaca tgggtttgcc actccgccgg ttgtgaccct ttcaggtaat gtttcacacc
      661 gggaagcttt tcttcgccaa gaatttgagt tggaggctgt gccccagcaa attccagctt
      721 gacagtttgg gctgtggagg gcgattcatt tgattcgaca accagaatat tttcggtcgc
      781 tgtgacaaaa aaggcatatc cctgtccacg tgccaaaaac tgaaccgacg gatcggtctg
      841 gcccagattg gtttcaaaac gaagtggaag tttgtccacc ggctgtgggc cagacgtcgt
      901 gggaccggcc acgggccggg tggcctgata cgagtgagcg gtgacggagg gtggatctga
      961 ctcacctgcc tgcctgtcgg gaggtgctga ttgatgcggt aaccgggacg tgtccggctt
     1021 gagtgccggt gacggagttg ccggtaaggg tgccgccggt ccaaaccaga gccaggcgct
     1081 ccccatcacc aggagcatgg tcacaatcat acgaaccacg gtttgtcccg tgaacggcca
     1141 caactgcacg ttttggagga atcgtttcat actgattttg cttctttccg tctggcggac
     1201 atagctccgc ccactcgcca ggtatgcgtt ttccctggtg atcgtcaggt tagcgatgct
     1261 gatggaaccc aggtcaggct tacattggcg cccaaccgtt caattcaaaa a
//
LOCUS       DAOQDO010002170         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127057_length_1310_cov_3.584861, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002170 DAOQDO010000000
VERSION     DAOQDO010002170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127057_length_1310_cov_3.584861"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..1136
                     /locus_tag="PKZ53_25790"
     CDS             462..1136
                     /locus_tag="PKZ53_25790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ43920.1"
                     /translation="MNQELIKQTTKRGLDVVLAGTALVVLSPLLLGLAGLIRYKLGGP
                     VFFRQRRPGLNGKPFELMKFRTMTSVCNQHGELLPDELRLTKFGQWLRSTSLDELPEL
                     INVFKGDMSLVGPRPLLMQYLPLYTTEQNQRHLVRPGITGWAQINGRNLLSWEAKFDL
                     DVWYVKHWSLWLDLRIIFLTLVKVVRREGISQPGTATATVFRGSAQVDGLHTGSSRDG
                     LCQKGI"
BASE COUNT          357 a          291 c          328 g          334 t
ORIGIN      
        1 gaaccgagaa tcagtccaaa tgctaaaaga aaaaacacga attttttcac aagttcctcc
       61 ttgtgcgatc tgtcagtaca gtggaaatgg ctaagtatga gcgggtaaat gttgttgatt
      121 tgggaatacc acgccagcca tcagaagtcg ttccatcaaa aaataattca atgctgaagc
      181 ctcagaatgc ctggttgtgg cacgaaggat gcactagaga gttcattgat gacaagtgat
      241 tgaatgcaaa agtgagattt gacctggcta aataccctga agaaagaagt ttttaagaaa
      301 tggatatttt tctaaattat ttaaaacaat gactttatat gtgaatctaa cgcctgatgg
      361 cgctcaggaa tggagttatt gtaataacaa atttcccaaa caagactgcc acaacccttt
      421 gaaaagtatc cattctgccc cagttttact ttcgaggcgc tatgaaccag gaattgatca
      481 aacaaaccac aaaacgagga cttgatgtcg tgctggcggg gacggcgctg gtcgtcctct
      541 ctccgcttct cctcggtctg gccggcctga tccgatataa actcggggga cccgtgttct
      601 ttcgccagcg ccgaccgggt ctcaacggca aaccatttga actgatgaaa tttcgaacca
      661 tgaccagtgt ttgtaaccaa catggcgaat tactgccaga tgaactccgg ttgacgaaat
      721 tcggacagtg gttgcgcagc accagtcttg atgaattacc agagttgatc aatgtcttca
      781 aaggggacat gagtctggtt ggcccgcgac cgttgttgat gcagtacctg ccgctttaca
      841 ccacagaaca aaaccagcga catcttgtcc ggccaggcat tactggttgg gctcaaatca
      901 atggccgcaa cctgctttcc tgggaagcaa agtttgatct cgatgtctgg tatgtcaaac
      961 actggtccct ctggcttgat ctgcgaatta tttttctgac gctggtcaaa gttgtcaggc
     1021 gtgaaggaat cagccagcca ggaactgcca ctgccacggt ttttcgagga agcgctcagg
     1081 tggatggact ccacacaggt tcaagccgcg atggcttatg ccagaaagga atctaaaatg
     1141 gaacgcaagc gaattgcaat tattggtgct ggtgggtgtg ccagggaagt cgcctggtta
     1201 attaaagaaa tcaaccgggt tcacccgata tatgagtttg ctggatatct ggtgagtgat
     1261 ctttcccgtc tgggcgaccg tgacagccgg gatcaggtgc tgggtgattt
//
LOCUS       DAOQDO010002171         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127109_length_1310_cov_2.414343, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002171 DAOQDO010000000
VERSION     DAOQDO010002171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127109_length_1310_cov_2.414343"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..170
                     /locus_tag="PKZ53_25795"
     CDS             18..170
                     /locus_tag="PKZ53_25795"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43921.1"
                     /translation="MRNIIANQWPEEEKMKRADEIIHNDDNHLIIPQILRLHQQFLLS
                     RPQATA"
     gene            180..1244
                     /locus_tag="PKZ53_25800"
     CDS             180..1244
                     /locus_tag="PKZ53_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ43922.1"
                     /translation="MTKRIITIAIILVIIFLLALPKLGLFDKKEIAPGAGATQVSALQ
                     VEGYSLQPVPFDNTLVITGSIQANESLELKSEASGKITRINFKEGGNVKKGELLVQIN
                     DEEIRAQIEKQRYMQKLNKDIEFRQRQLLKKDAISEEEYENALNRLNTTISDIKLLEA
                     QLQKTRVFAPFDGTIGLRYVSQGAYISPATVIATLYNSSPAKIEFAIPSRYSPMISTG
                     KKIRFTVENDTARVFSGEVYAIEPRIDEATRTLKLRALADNSKGQLIPGQFVKIDLVL
                     ASKQNALLIPTQAVIPGQTGQKVFITRAGKAQEVSIETGTRTNLKIEVLSGLTPGDTV
                     ITTGILQLRNEMPVKVVKVY"
BASE COUNT          386 a          338 c          292 g          294 t
ORIGIN      
        1 atcggtcgga aaatgatatc cgtaacatta tcgcgaacca atggccagag gaagagaaaa
       61 tgaaacgagc tgatgagatt attcataacg acgacaacca cctgatcatt ccacagatac
      121 tccgattgca tcaacaattt cttctatccc ggccacaagc cactgcataa ctcactcgaa
      181 tgacgaaacg catcatcact atagccatta ttcttgtcat catattcctg ctggccctgc
      241 caaaacttgg actctttgac aagaaggaaa tcgcaccggg tgcaggcgcc acccaggtta
      301 gtgcacttca ggttgaaggt tactctctcc agcccgttcc ctttgacaac accctggtga
      361 ttacaggctc cattcaagcc aatgagtctc tcgaactcaa gagcgaagca agcgggaaga
      421 tcacccgcat caatttcaaa gagggaggca atgtgaagaa aggtgaattg ctggttcaaa
      481 taaatgacga ggaaattcgc gcccagattg agaagcaacg gtacatgcaa aaactgaata
      541 aagacatcga atttcgtcag cgtcaactat tgaagaaaga tgccatcagt gaagaagaat
      601 acgaaaatgc tcttaatcga ctgaacacca cgatttctga catcaagtta ttggaagccc
      661 agcttcagaa gacccgcgta tttgccccat ttgatggaac catcggtttg cgttacgtca
      721 gccagggcgc ttatatttct ccggccacgg tgattgccac attatacaac agctcgcccg
      781 cgaagattga atttgccatt cccagccgtt actcccccat gatttccacg gggaagaaaa
      841 tccggttcac ggttgaaaac gacacagccc gtgttttcag cggagaggtc tatgccattg
      901 aaccccggat tgatgaagcc acgaggaccc tcaagctgcg ggctcttgcc gacaattcaa
      961 agggccagct gatccctgga caatttgtaa agattgatct cgtcttggcc tcaaaacaga
     1021 atgcactcct catacccacg caagccgtta ttcccggcca aaccgggcag aaggttttta
     1081 tcacgcgggc gggaaaagcc caggaagttt cgattgaaac aggaacgcgc acgaatctca
     1141 aaattgaagt actctctggt cttacgcccg gagatacggt aatcaccaca ggcatactcc
     1201 aacttcggaa cgaaatgccg gtaaaggttg ttaaggttta ttaaccgaaa aggtcatggc
     1261 tagtctttca accaccagta tcaaccgacc cgttctggcg atggtcatgt
//
LOCUS       DAOQDO010002172         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127314_length_1309_cov_2.034290, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002172 DAOQDO010000000
VERSION     DAOQDO010002172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127314_length_1309_cov_2.034290"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1309)
                     /locus_tag="PKZ53_25805"
     CDS             complement(<1..>1309)
                     /locus_tag="PKZ53_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43923.1"
                     /translation="GFTRCPDRWALVAAERTLRRIEKKKNANDQVPSFGSLSKEVADY
                     LTKHYFWLERLLCRMDSHCETALMSFDHLRKALKKLFDDRKLNNSDQFVRETIEILLE
                     VIRLAETSETSLQSIQSLLEKHYELTPTYGFTWIGNQGAQAQVNLLLGSRDQTFQQLG
                     ALQRLSRQIAESKSCQEQAVAKFTEYLGETKTLAEEQLQSLKTRMKDAFPVNFRQKRF
                     RIRSILLAAATDIINQICKMSKTSKPKSELQSLIEFALPPLLKSYSDKTVSETRDREK
                     LALFFLKQRLDPDSKASDEKYTQLKSDLRKLLARKLNRSGGPELNRNEEIGLVELPDV
                     SETDLDSIVDEALALLADDVYQEEEEKIIQAFRIQNLTAYARGMARTLFHKHLARKKA
                     EKAVSSSVRTTKLRLLDQNEKKKLRRQLETKTIRQCYLKCVHVV"
BASE COUNT          290 a          291 c          345 g          383 t
ORIGIN      
        1 accacatgga cacattttaa gtaacattgg cggatggtct tcgtctcaag ttgccggcgc
       61 aatttctttt tctcattctg atccagcagt cggagtttgg tcgttctgac tgaagatgaa
      121 acggcttttt cggctttttt ccgggccaga tgtttatgaa agagggtccg ggccattcca
      181 cgggcataag cggtcaggtt ttgaattcga aaagcttgaa taatcttctc ttcttcttcc
      241 tgataaacgt catcagccag cagggcaagg gcttcatcta cgatggagtc aaggtcggtc
      301 tcggaaacgt cggggagttc aactaaaccg atttcttcat ttcggttgag ttcagggcct
      361 ccagaacgat tcaatttccg ggccaggagc ttccgtaagt cagatttgag ctgggtgtat
      421 ttttcatcgc ttgcctttga gtctggatcc aggcgttgtt tcaggaaaaa gagcgcaagc
      481 ttttctctat cccgggtctc acttacggtt ttatcggaat aggatttgag caaaggggga
      541 agggcaaatt caatcaggct ttggagttcc gatttgggtt tggatgtttt cgacattttg
      601 catatctggt tgatgatatc agtggccgct gcgagaagaa ttgaccgtat ccgaaatcgt
      661 ttttgtcgga aattcaccgg gaatgcgtct ttcattctgg ttttcaatga ttgcaattgc
      721 tcttcagcca gggttttggt ttcgccgaga tattcggtga atttggcaac ggcctgttcc
      781 tgacaacttt ttgattcggc aatttggcgc gacagacgct ggagggcgcc caattgttga
      841 aatgtttggt cccgggaacc aagcagcaaa ttgacctgcg cctgcgctcc ctggttgccg
      901 atccaggtaa acccgtaggt aggggtcagt tcatagtgtt tttcaagcaa ggattgaatg
      961 ctctgtaacg atgtctcgga tgtttcggcc agtcggatga cttcaagcaa aatttcaatt
     1021 gtttcccgaa cgaactggtc tgaattattc agtttgcgat catcgaacaa ttttttgagc
     1081 gccttgcgca ggtggtcaaa actcatcagg gccgtctcac agtgcgaatc catccggcac
     1141 agcagtcgct caagccagaa ataatgcttg gtgaggtaat ccgccacctc tttggagagc
     1201 gacccaaagg atggaacttg atcgttggcg ttttttttct tttcaatccg gcgcagagtc
     1261 cgctcagcgg caacgagtgc ccagcggtct gggcagcggg taaagcctt
//
LOCUS       DAOQDO010002173         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127409_length_1308_cov_3.853152, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002173 DAOQDO010000000
VERSION     DAOQDO010002173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127409_length_1308_cov_3.853152"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..>1308
                     /locus_tag="PKZ53_25810"
     CDS             419..>1308
                     /locus_tag="PKZ53_25810"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNJ43924.1"
                     /translation="MVGPHPSSQAFQKQTPPATTTKTPSARDIACYQALLNLESPYSL
                     LCVAAHPDDEDGASLVYYRMKYGVNTASITATRGEGGQNAVGTELNEALGVIRSYEMQ
                     AAAAYQESPNYNLGLEDFGFSKTSREALARWGHDEALRRLVGLIRKLRPDVVITNHDT
                     KTGHGHHQAVGLLLQEAFEAAADPNQFSDQIIDGVLPWQIRRLYERAFGETKPDISFE
                     VNQVDPYRQGSYVQITYDALKMHATQGPWPSADLSRPVTRRFRLIKSKNAGDLPAGSD
                     NLFAGLESQSKPDFAEVYAGV"
BASE COUNT          322 a          341 c          340 g          305 t
ORIGIN      
        1 gttaacgcga agaattatag ctaatgcact atttggttat ggttttagca actatgagga
       61 ctctgtcagt cgggcagatg attcagtgct caagtccaga aaacagccat ttccaggttg
      121 aattgcacag ttcaccagat ttcactaacg ttgcctgatc aagttcagtg gttgagcttt
      181 tgctggcgaa aaatacacat tcacaccaca cacaatcata tcaactcttg aaaagcctgt
      241 gcttctgcca gaggtcactt ccgccagagt gtttcttcag gcatcaataa ggaactttgc
      301 ccgcatgaaa gatcaattca gcgcaatcga tcaacaaacc cagttaccgc gttttcgcgg
      361 gtccatactg atccgatggg gtttgttcgg ggtactcatt ctgactctca tcagtggtat
      421 ggtggggcca cacccttcca gtcaggcatt tcaaaagcaa acaccgccag ccacgaccac
      481 gaaaacgccg tcagccagag atattgcctg ttaccaggcg ctgctcaatc ttgaatcacc
      541 ctattcattg ctctgtgtgg cagcgcatcc ggatgatgaa gatggcgcct cgctggtcta
      601 ttaccggatg aaatatggcg tcaataccgc atctatcacg gccacacgcg gcgaaggcgg
      661 gcaaaatgcg gttgggacgg aattgaacga ggcgctcggg gtcattcgat cttatgaaat
      721 gcaggcggca gccgcgtacc aggagtctcc gaattacaac cttggcctgg aagactttgg
      781 gttttccaaa acgtcccgag aagcgctggc ccggtgggga cacgacgaag cactgcgccg
      841 gctggtggga ttgattcgaa aattgcggcc agatgtggtg attaccaacc acgacaccaa
      901 aaccgggcat ggccatcacc aggcagtcgg cctgttgttg caagaagcgt ttgaggccgc
      961 cgccgatccc aatcaatttt ctgaccagat tattgatggc gttttgccct ggcaaattcg
     1021 gcggctgtac gagcgtgctt ttggtgaaac aaagccggat atctcctttg aggtcaatca
     1081 ggtagatcca tatcggcagg ggagctatgt ccagattacc tatgatgcgc tcaagatgca
     1141 cgccacccag gggccctggc ccagtgccga tctcagccgt ccggtaaccc gccggtttcg
     1201 cctgatcaaa agcaaaaatg ctggcgacct gcctgctggt agcgacaacc tgtttgccgg
     1261 tttagaaagc cagtcaaaac cagattttgc ggaagtctat gctggggt
//
LOCUS       DAOQDO010002174         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127487_length_1308_cov_2.299282, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002174 DAOQDO010000000
VERSION     DAOQDO010002174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127487_length_1308_cov_2.299282"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKZ53_25815"
     CDS             <1..274
                     /locus_tag="PKZ53_25815"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNJ43925.1"
                     /translation="FYAVHSERPFFKDLVGFMTSGPSVVLALEKEDAVKAWRDLMGPT
                     NSKNAPQGTIRGDFGTDVERNASHGSDSQENAAIEVAFFFRKSELV"
     gene            complement(367..>1308)
                     /locus_tag="PKZ53_25820"
     CDS             complement(367..>1308)
                     /locus_tag="PKZ53_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HNJ43926.1"
                     /translation="EIVFSGGNLTRDEFEQMLWWQVATLNLDSLDQLRSLIDVYRNWP
                     EKKYLPRLGFRLNLPDLTGKTRTGVSPAEFPAALELTKGSELQVTGLHFYRGTGTNST
                     SSFTSVIAEVLKAGQQLPAWNYLDFGGGFGYAYHADRPGFEWKTFGDELTCQLLELSQ
                     PVNLVIEPGRAAIARCGTLLTRVVSAKWQEDRQIVGTDTTVGNIAVLAVHGGHREVSF
                     WKDSSSPRFSTDVCGNSTFSRDYLSRDAQLPELKAGDVLGILDVGAYGYAMSSHFLHR
                     PRPAEVLVGQGSPRLIREREGYEVLLAGQVMGEIVKS"
BASE COUNT          337 a          371 c          294 g          306 t
ORIGIN      
        1 attttacgcc gtccacagcg aacgtccctt ctttaaagat ctggtcggct ttatgacctc
       61 tggaccgtca gtggtcctgg cactcgaaaa agaagacgcc gtcaaagcct ggcgcgatct
      121 gatggggcca accaattcaa agaatgcccc acaaggcacg attcgcggtg actttggcac
      181 cgacgtggaa cgcaacgcca gccacggttc agattcgcaa gaaaatgccg ccatcgaagt
      241 ggctttcttc ttccgcaaat cagagttggt ctgaacagta gcgaaggagt gaagtagctc
      301 tgcattgatt ctcaggttgt tttctttcaa ccctttcatt tctctgatgt tgactacttc
      361 gctccttcac gacttcacta tttcacccat tacctgcccc gcgagtaaca cttcataccc
      421 ttcacgttca cgaatcaaac gcgggcttcc ctgccctacc aacacttcag ccggtcgtgg
      481 acggtggaga aaatgcgacg acatcgcata gccataagcc ccaacatcca gaattcccaa
      541 tacatcccca gcttttaatt ccggcaattg cgcatcacgg ctcaaataat cacgcgagaa
      601 cgtcgaattt ccacagacat cggtggaaaa tcgtggcgag gaactgtctt tccagaagga
      661 aacttcccga tggccgccgt ggacagccag aaccgcaatg tttccaaccg tggtgtcagt
      721 tccaacaatc tggcgatcct cttgccattt tgctgaaaca acacgggtta acagtgtccc
      781 gcatctggca atcgcagccc gcccaggttc aatgacaaga ttgacaggtt ggctcaattc
      841 taaaagctgg caggtgagtt catcgccaaa tgtcttccac tcaaaccctg gacggtcagc
      901 gtgataggcg tacccgaagc ctccgccaaa atcgaggtag ttccaggccg gcaattgttg
      961 tccggctttc aaaacttcgg caatgacact ggtaaaagaa gaagtcgaat ttgtccccgt
     1021 gcctcgataa aaatgaaggc ccgtcacctg gagttcagaa ccttttgtaa gttcaagcgc
     1081 agccggaaac tcagccggag aaaccccagt tcgtgttttt ccagtcaggt caggcaggtt
     1141 taatcgaaaa ccaaggcgtg gcagatattt tttctcaggc cagtttcgat acacatcaat
     1201 caatgaccgc aactggtcca gactgtccag gttcagcgtt gcgacctgcc accagagcat
     1261 ttgttcaaat tcatcacgag tcagattccc gccgctaaac acaatttc
//
LOCUS       DAOQDO010002175         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127605_length_1307_cov_4.293930, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002175 DAOQDO010000000
VERSION     DAOQDO010002175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127605_length_1307_cov_4.293930"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKZ53_25825"
     CDS             <1..1113
                     /locus_tag="PKZ53_25825"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HNJ43927.1"
                     /translation="LRRLFSRYLGASPLTVAQTRRIHFAKQLLDETKLPITEIAFAAG
                     FGSIRRFNDAMLQTYQRSPRDLRRDKQDHEFSPGTGITIKLPFAQPYDWPALVRFLSA
                     RTTAGIESISEAGYSRTIELAGQQGLVQVKPVAGQRYLLATIYFPEVKLLANIVERLR
                     RLFDLNANILEITTHLQTDPVLRPLVESQPGLRVPGAWDEFELAVRAILGQQVSVQAA
                     TTLAGRLVERFGTPLSETLAELSGPLLATFPRPEVLASADLTHIGLPQARAAAISALA
                     QKVADQPAFFQSFQTLEDAVEQLCQLPGIGPWTANYIAMRALGEPDAFPASDLGIKHA
                     LEQLDPDFDPKKVTERAEIWRPWRAYATMYLWNPGE"
BASE COUNT          300 a          340 c          341 g          326 t
ORIGIN      
        1 ctgcggcgac tgttttcacg ctacctgggt gcctccccac tcacagtcgc ccagacacgg
       61 cggattcatt ttgcgaaaca gttgctggat gaaacgaaac tgccgattac tgaaattgcg
      121 tttgcggctg ggtttggcag cattcgccgg tttaatgacg cgatgctcca gacctatcag
      181 cgttcaccac gggatcttcg acgggataag caggaccatg aattttcacc tgggacgggc
      241 attacaatta agcttccgtt tgctcagccg tatgattggc cggcactggt gcggtttttg
      301 tcggcgcgga caacggctgg tatcgaaagc atttcggaag ctggctacag tcggacgatt
      361 gagctggcgg gtcagcaagg actggttcaa gtcaagccag tggctggtca aagatatttg
      421 ctggcaacta tttattttcc tgaagttaag ctgctggcga atattgttga acgtttgcgg
      481 cgactctttg atctaaatgc caacattctc gaaatcacga cccatcttca aactgacccg
      541 gtgttgcgcc cactggtcga aagccagcca ggacttcggg tgccaggtgc ctgggatgaa
      601 tttgaactgg cggtgcgggc cattttgggc cagcaagtca gtgttcaggc ggcaacgacg
      661 ctggctggaa ggcttgtcga acggtttgga acgccactgt ccgaaaccct ggccgaactt
      721 tccggcccct tgttggccac atttccacga ccggaagttc tcgccagtgc tgacctgacc
      781 cacattggac tcccacaggc cagagcagcc gcaatttcgg cgctggctca aaaagtcgcg
      841 gatcagccag cattctttca aagctttcaa acgcttgaag atgccgtcga acaactctgt
      901 cagcttccag gtatcggccc ctggacggcc aactatatcg cgatgcgggc acttggcgaa
      961 ccagacgctt ttccagccag tgatttggga atcaagcacg ccctggaaca actcgaccct
     1021 gactttgatc ccaaaaaagt aactgaacgc gctgaaatct ggcgcccctg gcgggcctac
     1081 gccacgatgt acctgtggaa cccaggagaa taaagaatga agaatgaaga ataaagaatg
     1141 aagaaaattc gttgggcttg atgcctggtg tttggttttt cgtgcttggt gacttgttct
     1201 tcgtgctatg aaagtatggt tccaaaatac caggtgccgg gtaccaagtt cattcttcat
     1261 tctcaattct caattctcaa ttcttcattc tcaattcttc attcttc
//
LOCUS       DAOQDO010002176         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_127634_length_1307_cov_3.038339, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002176 DAOQDO010000000
VERSION     DAOQDO010002176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127634_length_1307_cov_3.038339"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PKZ53_25830"
     CDS             <1..173
                     /locus_tag="PKZ53_25830"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ43928.1"
                     /translation="ELSPKLRTVVVLRYVELLSYEEIAEVLGCSVGTVSSRLHRALTE
                     MEAHLRPLKRML"
     gene            245..973
                     /locus_tag="PKZ53_25835"
     CDS             245..973
                     /locus_tag="PKZ53_25835"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ43929.1"
                     /translation="MWCWRIDIQRKLMAYLDSNLNPPGVFKVEQHLLDCASCRGQVEH
                     LRRGQRFAMAIERHRAPETGWEALEAALDAIPVAERAKPVAVASHIRWLSWPGMALTT
                     GLLVGGVLVGALWLAVNTPSTPSLKTKILESVEPEEESQAFHEVSISGIESNTEPHIV
                     VEGVVNEVKIDADGDLTFRLVEKVNQATPFVVCEVLNPHQIKPPSPGDRVRVYGVSRY
                     DNKHTHQWYEVHPVMNIELVRNEK"
     gene            1115..>1307
                     /locus_tag="PKZ53_25840"
     CDS             1115..>1307
                     /locus_tag="PKZ53_25840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ43930.1"
                     /translation="MAGLISLSPLGTPSTTYAISRLQGQTSVTGVVKDRTGKAYPNAK
                     VTLLNSQQSIIATTQADSDG"
BASE COUNT          344 a          330 c          339 g          294 t
ORIGIN      
        1 ttgaactctc accaaaacta cggacggttg ttgttttacg ctatgtcgag ttactgagtt
       61 acgaagaaat tgccgaggtg ctggggtgca gcgttggcac tgtctcatca cgccttcatc
      121 gggccttgac tgaaatggag gcgcatttgc gtccactcaa acggatgctt tagatgccac
      181 ccgcgtcagg acctgtggtg atcaacccaa accaggtgaa tacaatgaaa tgtgaggctg
      241 gaatatgtgg tgctggagaa ttgatattca acgaaaattg atggcctatc tggattcaaa
      301 cctgaatcca ccgggtgtat tcaaagttga acaacatttg ctcgattgtg cctcctgtcg
      361 ggggcaggtt gaacatctgc gccggggcca gcgctttgcc atggcgattg aacgtcaccg
      421 ggcacccgag acaggctggg aggctctgga agccgcactc gatgcgattc ccgtcgcgga
      481 gagagccaaa ccagtggcgg tggcttccca catccgatgg ttaagctggc ctggaatggc
      541 actgaccacc ggattgctgg ttggcggtgt gctggtgggt gcactgtggc tggctgtcaa
      601 caccccctcc actccctctc tgaaaaccaa aattctggaa agtgtagaac ctgaagaaga
      661 aagccaggct tttcacgaag tctcaatttc gggcattgaa agcaataccg aaccccacat
      721 tgtggtggaa ggcgtggtca acgaagtcaa aattgatgcc gacggagacc tcacctttcg
      781 tctggttgaa aaggtcaatc aggccacgcc ctttgtggtg tgtgaagtcc tcaacccaca
      841 tcagatcaaa cctccctcac cgggtgaccg ggtccgcgtg tatggtgtca gtcgctatga
      901 caacaagcat acccatcagt ggtatgaagt ccatccggtg atgaatattg aactggtcag
      961 aaatgaaaaa taagcccagg ccgcaaaaac cgggtttcgg aaaaataaag tcagcagggt
     1021 caacctgaga acaattacat cttgcacagg aaaacggagc tttttacttt ttatgaaaaa
     1081 acctatgtct cgacagtggg tatggtgtct actgatggca ggtctcatca gtttgtcccc
     1141 acttggaaca ccatcaacaa cctacgccat ttcccgatta caaggacaaa ccagtgtcac
     1201 cggggtcgtc aaggatcgga ctggaaaagc ttacccaaat gccaaagtta cgttgctcaa
     1261 cagccagcaa tccatcatcg ccaccaccca ggcagattca gacggaa
//
LOCUS       DAOQDO010002177         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128219_length_1304_cov_2.499600, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002177 DAOQDO010000000
VERSION     DAOQDO010002177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128219_length_1304_cov_2.499600"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKZ53_25845"
     CDS             complement(<1..319)
                     /locus_tag="PKZ53_25845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ43931.1"
                     /translation="MRAAQAGNRVAFRQLYDQYRDAVYNLIVYLIGNRLEAEDLLQNT
                     FLKAYRALAGFRFEASFKTWILRIAHNECTDALRRSHPDMVPIEDLLGSQEEIDHSRL
                     PDDL"
BASE COUNT          323 a          265 c          329 g          387 t
ORIGIN      
        1 ggagatcatc ggggagacgc gaatgatcaa tttcttcctg actgcctaaa aggtcttcga
       61 tgggcaccat atccggatgg cttcgacgca gcgcatccgt acactcatta tgggcaatcc
      121 gcaggatcca cgtcttaaag ctggcttcaa accgaaatcc agccagggcc cggtaggctt
      181 tgagaaatgt gttttggagc aggtcttcgg cttccaggcg gtttccaatg agatacacaa
      241 tcaggttata gaccgcgtcc cggtactggt catacagctg ccgaaacgca acccggttcc
      301 cggcctgtgc ggctcgcacg cagtcaaact cactctcgag cggaccatgg gattgaaatt
      361 ggatatgggt attcacgtgc ttgtttggag atgagtgctg agatttcagg atattttttg
      421 acggtatcta ggggaataga cgtcgtcggt tgatatttct tccctgactt attcactttt
      481 tttgaaattt tttgaaatta tttgcaactt ccaaatttca ctcgatgaat ttggttaaaa
      541 aaacgcgctt tgaaaaaaaa ttgaaaaagc ttaaatatgt tgccgataaa aacgaggcaa
      601 aatggaattt attttaatac ttttgatcca taatacggct gcttttcgct ggttcctgct
      661 tctaccggat tttgtgtttt gccagagcag cgttgggaat aaaggactgg tcagtaccac
      721 ctgcgtgagc gggtggggtt tctaccggat tttgtattac gccagagtag tgttgggaat
      781 aaaaaacagg tcagtaccac ctgcgtcagt gggtggatcg gtcggctagc caatcccgcc
      841 cgctcacgca ggtggtactg attcgtcggt gcttctgatt tgtctccagc accaacgaaa
      901 tgagtataaa aatataagtt tttacaataa aaaatttgcg gccaaataaa ctcttatttt
      961 aggagtttgt aaacgaataa tttctgtctc ccggaatgta cgaatttcct cggcaaaatc
     1021 tttcagacgt caagtgcctt gctgtaccgg ccagccccaa aaactttgtg atgaggtttg
     1081 tggagggaaa cgggggtgat ttggttttgg ttgtttttcg gggtgtgagc cctgcgtgaa
     1141 atagcgttta atcgcgttta tttggctttc ctgaggtttt ttgagcaaaa acgagttagg
     1201 tattgaacct gatttgaaaa atcgctctac gggcatttta cgcgattttc ctggttttgg
     1261 ctggtctggg ttgaagggaa cgtcaccaaa ctggagttgc gttc
//
LOCUS       DAOQDO010002178         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128229_length_1304_cov_2.395516, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002178 DAOQDO010000000
VERSION     DAOQDO010002178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128229_length_1304_cov_2.395516"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PKZ53_25850"
     CDS             <1..1162
                     /locus_tag="PKZ53_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNJ43932.1"
                     /translation="VNYDVFKNIDEVSYGTTNIDYSAALYDEFQKIRSYKSTRFVIDP
                     TGFTAETGEDEAYKEVKIDLPESWVRGFLQVSSAMSLPTTSFTLHPMDIHNLCFVLRR
                     HKERQGPRSLRYHLTPGEPVKIVLEPWGTEILCPRSPYLGKAQDTIRVWGRRRIHILE
                     RLIPVAKSFTVHLMGTGLPSFYIADLGDMSFTLGLSGWTTNDWAKAGNFDLMAPRAEV
                     DDYTKRLVFEALKRHWAEHPDQLAERLNLDRAVVLGALGAFTQAGRAIYDLNKGVYRA
                     RELSREPLPMDKLRFANPREESATRLLVKNVVLVTASQDGQGNLVLQGNIRENSHMYH
                     TSVTIDQDERMINASCGCNWHQHNKLYKGPCEHLLATRLQYRCQTESSRAIA"
BASE COUNT          328 a          321 c          350 g          305 t
ORIGIN      
        1 cgtcaactat gatgtcttca aaaacattga tgaagtttcc tacggtacga ccaacattga
       61 ttattcagcc gcgctctatg atgaattcca aaaaatccgc agttacaaat caactcgctt
      121 tgtgattgat ccaactgggt ttacggctga gaccggcgaa gatgaagcct ataaggaagt
      181 caaaattgac ttgccggaga gctgggtgcg tggatttttg caggtcagtt cggcgatgtc
      241 cctgccgacc acatcgttta cgttgcaccc gatggacatt cacaatttgt gttttgtgtt
      301 gcgacggcac aaggaacgcc aggggccacg gtcactgcgc tatcatttga cacccggcga
      361 accggtcaaa attgtgcttg aaccctgggg aacggaaatt ctttgccctc gctcgccata
      421 tctgggcaag gctcaggata cgattcgcgt ctggggccgc cggagaatcc atattttgga
      481 gcggttgatt ccggtggcga aatccttcac ggtccatttg atgggaaccg gattaccatc
      541 cttttatatt gctgatctcg gggatatgtc gttcacgctt ggtttatcgg gctggaccac
      601 caatgactgg gccaaagccg ggaactttga tttgatggcg ccccgagccg aagttgacga
      661 ctataccaaa cgcctcgttt ttgaagcgct caaacgtcac tgggccgagc atcctgacca
      721 acttgcggaa cggttaaacc tggaccgggc ggttgttctc ggtgcacttg gtgcatttac
      781 tcaggcgggt cgggccattt acgatctcaa taaaggcgtg tatcgggcac gtgaactcag
      841 tcgcgaaccg cttccgatgg acaaattgcg gtttgctaac ccgcgtgaag aaagtgccac
      901 gcggctactt gtcaaaaatg tagtgttggt gactgccagc caggatggac aggggaatct
      961 ggtcctgcaa ggcaacattc gcgagaatag ccacatgtac cacacctcgg tcactattga
     1021 tcaggacgaa cgcatgatca atgcttcctg cggctgtaac tggcatcagc acaacaagtt
     1081 gtacaaaggg ccttgtgaac acctgctggc gacccggctg caataccggt gtcaaacgga
     1141 atcatcgagg gccattgctt gaatataggg cttggggcta agggcctggg gttgacagaa
     1201 ccaaatagtg gttcgtggtt cgtggttaga aataaattct ttcgaagaag aaccaagaac
     1261 caggcaccaa gcaccaagaa ccaagaacca agaaccagga acca
//
LOCUS       DAOQDO010002179         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128240_length_1304_cov_2.301041, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002179 DAOQDO010000000
VERSION     DAOQDO010002179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128240_length_1304_cov_2.301041"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..766
                     /locus_tag="PKZ53_25855"
     CDS             17..766
                     /locus_tag="PKZ53_25855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ43933.1"
                     /translation="MSFLDYFSGHSDLYRSARPTYPDELFAWIASQAPERERVWDCAT
                     GNGQAAAGLACHFETVLATDASARQLANAVQVSNVTYLLQPAEKTEFPDNYFDAVCVA
                     QALHWFDHDRFYPEVHRVLRPGGIFAAWVYSEVFVDLVLDPILQEQIITVIAPYWSPQ
                     NVHSHNGYRDLPFPFEPVEAPRFTITVDWNLPQLMAFIETWSATQKLISEQGTGFLEQ
                     AIQKIAESWGDPGTLRQVNMPIALKCGRLTS"
     gene            787..>1304
                     /locus_tag="PKZ53_25860"
     CDS             787..>1304
                     /locus_tag="PKZ53_25860"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNJ43934.1"
                     /translation="MPVTQPSPSKPEDPFPELYFANPAIWAIVLLPGVFLGMTAIFLI
                     AMWDRLPGRWVIHWGASGQPDGWATKTPFWVFFPIGFGFGLWLLINTMGWITGTLLRK
                     HKTLPVDAAAELAVANMVLIHTLAAGLASVFMILAVWLPLGRPDNPTGMILVIVGILA
                     LAMVSGIRRLGAV"
BASE COUNT          261 a          294 c          384 g          365 t
ORIGIN      
        1 ctgaaccctg attcccatgt cttttcttga ctatttttcc ggacattctg atttgtaccg
       61 gtcggcgcgg ccaacctacc cggatgagct gtttgcctgg attgcatcgc aagcgccaga
      121 gcgtgagcgt gtgtgggatt gcgccaccgg caatgggcag gcagcggctg gattggcttg
      181 tcattttgag acggttttgg caacggatgc gagtgccaga cagcttgcca atgcagttca
      241 ggtgagtaat gtgacctatt tgctgcaacc agctgaaaaa actgagtttc ctgataatta
      301 ttttgatgcg gtgtgtgtgg ctcaggcact tcactggttt gatcatgatc ggttttatcc
      361 agaggttcat cgggtgttga gacccggcgg tattttcgcg gcctgggtgt atagtgaggt
      421 gtttgttgac ctggtgttgg atccgattct gcaagagcag attatcacgg tgattgcccc
      481 gtactggtca cctcagaatg tccattcgca caatggttac cgtgatttac cctttccttt
      541 tgaaccagtt gaagcgccgc gttttacgat tactgttgac tggaacttgc cgcagttgat
      601 ggcttttatc gaaacctggt cggcaacgca aaagctcatt tccgagcagg gaactggttt
      661 tttggaacag gcaattcaga aaatagctga aagctggggg gatccaggaa cgttacggca
      721 ggttaacatg ccgattgccc tcaaatgtgg gcgacttaca tcctgaatat atccaaggag
      781 ttcgatatgc cagtaactca accatcgcca tcaaagcctg aagacccatt tcctgaactc
      841 tattttgcca atccggcgat ctgggccatt gtgttgctgc caggggtgtt tttgggaatg
      901 actgcgattt tcctgattgc gatgtgggac cggttgccag ggcgatgggt gattcattgg
      961 ggcgcctccg gccagccaga tggttgggca accaaaaccc cgttttgggt gttttttcca
     1021 attgggtttg gttttggatt gtggttgctg atcaatacga tgggatggat taccgggacg
     1081 ctcctgcgca agcacaaaac attgccggtt gatgccgcgg ctgaactggc ggtggcgaat
     1141 atggtgttga ttcacactct ggcggcaggg ctggccagtg tctttatgat tctggcggtc
     1201 tggttgccgc ttggacgacc cgacaaccca accggaatga ttctggtgat tgttggaatc
     1261 ctggcgctgg ccatggtgag tggcattcga cggctggggg cggt
//
LOCUS       DAOQDO010002180         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128382_length_1303_cov_3.177083, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002180 DAOQDO010000000
VERSION     DAOQDO010002180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128382_length_1303_cov_3.177083"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKZ53_25865"
     CDS             <1..205
                     /locus_tag="PKZ53_25865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43935.1"
                     /translation="GSKDEKDTKDRRDINSKTRNPEPATRNPQPHLRSDTPDTLDYGF
                     LRKSTQLLFAHCLGYASSYSSLS"
     gene            334..>1303
                     /gene="hppD"
                     /locus_tag="PKZ53_25870"
     CDS             334..>1303
                     /gene="hppD"
                     /locus_tag="PKZ53_25870"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNJ43936.1"
                     /translation="MTTPKESLVGKATPLAVNDFDHMEYFVGNAKQAAFFFRFALGFD
                     IVAYRGPETGTRETVSYVLKQNDINLIITGAFLPEHPVTEHVRKHGDGVNAVAFKTAD
                     AFGDYEKAMARGAESVQEPTTLEDEHGTVKTAAIKAYGDTVHRFIEREGYKGTFLPGF
                     RDLYLPSPNRAGLIRIDHTVANVGWNEMETWVEYYERIFGFFQFRHFDENDISTEFTA
                     LRSKVMASPTLGIKLPINEPAEGRKRSQIEEYVDFYQSAGVQHAAITTADILSTVRQL
                     RANGIEFQTVPGSYYDTLLNRVGPIREDVEILRELGILVDKDDSGYL"
BASE COUNT          342 a          347 c          321 g          293 t
ORIGIN      
        1 aggcagcaag gacgaaaaag acacaaagga cagaagggac ataaattcga aaacccggaa
       61 cccggaaccc gcaacccgca acccgcaacc ccacctccgc agcgacacgc cagacacgct
      121 cgactacggt tttcttcgaa aaagtaccca gttgcttttc gcccattgct tgggatacgc
      181 ttccagctat tcatccctct cttgacccag cctcaactga tatcagttcc tcccaaaggc
      241 tgattttcaa gagaaattac atcgaacttc atcgctttct ttgaaaatgt atctcgcgag
      301 ccgagacccg gtggcgagga aaggaatttt tgtatgacaa ctcccaaaga atctctggtt
      361 ggaaaggcta cgccgctggc agtgaatgat tttgaccata tggaatattt tgtcggcaat
      421 gccaaacagg ccgccttctt ttttcgattt gccctgggtt ttgacatcgt ggcctaccgg
      481 ggtccagaaa ccggcactcg cgaaaccgtt tcgtatgtcc tcaagcaaaa tgatatcaac
      541 ctgattatca ccggcgcgtt tttacccgag catccagtga ccgaacacgt tcgcaaacac
      601 ggggacggcg tcaatgccgt cgcctttaaa acggctgatg cgtttggcga ctatgaaaaa
      661 gcgatggctc ggggagcgga atccgtgcag gaaccaacca cgctcgaaga tgaacacggc
      721 acggtcaaaa cggcggcgat caaagcctat ggcgacacag tccaccggtt tatcgaacgc
      781 gaaggctata aaggcacttt cctgccggga tttcgcgatc tgtatctgcc ctctcccaac
      841 cgggcaggac tcatccgcat tgatcacaca gtagccaatg tgggctggaa tgaaatggaa
      901 acctgggttg aatattatga acgcattttt gggttcttcc aattccgcca ctttgacgaa
      961 aacgacatca gcactgaatt cacggccttg cggtcaaaag ttatggccag cccgacgctg
     1021 ggcatcaaat taccgatcaa tgagcctgcc gaaggccgca aacgctcgca aattgaagaa
     1081 tacgttgatt tttaccagtc agcgggcgtc cagcatgcag ccattaccac ggctgatatt
     1141 ctctcgacgg tgcgtcagtt gcgggctaac ggcattgagt ttcaaaccgt gccgggtagc
     1201 tactatgaca cattgctgaa tcgggtcgga ccaattcgcg aagatgttga aattttgagg
     1261 gaactgggca ttctggttga caaggatgac agcggctatt tgt
//
LOCUS       DAOQDO010002181         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128429_length_1303_cov_2.413462, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002181 DAOQDO010000000
VERSION     DAOQDO010002181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128429_length_1303_cov_2.413462"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PKZ53_25875"
     CDS             complement(<1..1291)
                     /locus_tag="PKZ53_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNJ43937.1"
                     /translation="MPKLQASTPQPSTSGVQILGIRHHGPGSARSVRQALEAMQPDII
                     LVEGPPDGDSILPLLTHAEMKPPVALLIYAVDDPKQAVFYPFAVFSPEWQAIQFGLKH
                     EIPVRFMDLPQANRLAITTEPESSALSPVPSAPSDSQPSSPEPGTRNPEPGTPDPLSW
                     LAQAAGFEDSERWWEYMVEHRDNTTDVFAAILEGMTALRSSGIGFRESSTEVALEAQR
                     EAFMRQTIRATQKEGFQKIAVVCGAWHAPALAEMPPAKSDAELLKGLPKVKVSATWVP
                     WTYSRLSFQSGYGAGVESPGWYHHLWHIREAATTGVSPTTVTVRWMSRVAQLMRAEDL
                     QASVAHIIEAVRLAESLATLRGCPLPGLAELNDAAQAIMCFGNDAPMRLIHEKLVVGE
                     LLGTVPDETPMVPLQQDLQREQKRLRLQPEAAHKDMDL"
BASE COUNT          279 a          364 c          367 g          293 t
ORIGIN      
        1 ccagatccat atctttatgt gctgcttcgg gttgcagacg gagccgcttc tgttcacgct
       61 gtaaatcctg ctggagtggc accattggcg tttcatctgg aaccgtgcca agtaattcgc
      121 caaccaccag tttttcgtga atgagccgca tcggtgcgtc attgccaaag cacataatgg
      181 cctgcgccgc gtcgttgagt tcagccagtc ccggcaacgg acaacctctc aacgtagcca
      241 gtgattcagc caatcgaacg gcttcaatga tgtgcgccac cgacgcctgc aaatcttcgg
      301 ctcgcatcaa ctgagctaca cgcgacatcc agcggacggt aactgtcgtt ggtgaaacac
      361 ctgtcgtcgc ggcttcgcga atgtgccaga gatggtgata ccagcccggc gattcgaccc
      421 cagcgccata tccgctttga aaactcagtc ggctgtaggt ccacggcacc caggtggcac
      481 tgactttaac tttcggaagc cctttcaaca gttcagcatc actctttgcc ggtggcatct
      541 ctgccagggc cggagcgtgc caggccccac acaccacagc aatcttttgg aagccttctt
      601 tctgggtcgc ccgaatcgtc tgccgcataa aggcttcgcg ttgggcttca agggcaactt
      661 cggttgaaga ttcgcgaaac ccaatacccg aacttcggag cgccgtcatg ccttctaaaa
      721 tggcagcaaa cacgtcggtg gtgttgtctc gatgctcgac catgtattcc caccagcgtt
      781 cgctgtcttc aaatccggca gcctgggcca gccaggagag aggatcggga gttccgggtt
      841 ccgggttccg ggttccgggt tccgggcttg aaggttgaga atcagatggg gctgagggta
      901 caggactgag agctgaagat tcaggttctg tcgtgatagc cagccggttg gcttgcggga
      961 gatccataaa ccgaactgga atttcgtgtt tgagtccaaa ttgaatcgcc tgccattccg
     1021 gagaaaatac ggcaaacgga taaaacacgg cctgctttgg gtcatccacc gcataaatca
     1081 gaagtgccac cgggggcttc atctcagcgt gggtgagcaa cggcaggatg gagtcaccat
     1141 ccggagggcc ttcaaccaga atgatatccg gctgcatggc ttcgagtgcc tgccgcacgc
     1201 tccgcgccga acccggcccg tgatggcgaa tcccaaggat ttgcactcca gaagttgaag
     1261 gttgaggggt ggaagcttga agtttgggca tggtgttctc ttt
//
LOCUS       DAOQDO010002182         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128837_length_1301_cov_2.154093, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002182 DAOQDO010000000
VERSION     DAOQDO010002182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128837_length_1301_cov_2.154093"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..>1301
                     /locus_tag="PKZ53_25880"
     CDS             437..>1301
                     /locus_tag="PKZ53_25880"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43938.1"
                     /translation="MSLLEQIFFLPQRLCRLCCQINGLWVILLVLGWGHTGLALDPAK
                     TLSQYGYDEWQDGLPQNTVHTVVQTQDGYLWLGTYEGLVRFDGAQFKVFDRSTVPQIK
                     NNSIWVVRESPDQTLWIGTLGGGLLSYRNHQFVGYDTAEGLVSSFVYALCPSRDGGLW
                     IGTNQGLCKLDQGQFKSYPEHKELAGKLIRALYEDKNGRLWVGTDTQGLICLEAGRTT
                     VYSTASGFPSNSIYAVLEDAQGGLWIGTYGGGLCYLRDNRWTAYTTRDGLPDNLIRSL
                     YLDRQGVLWIGT"
BASE COUNT          349 a          314 c          313 g          325 t
ORIGIN      
        1 gatcaaaagt gcttttaatt caaaattttg aaacaattaa aacctgcatc cgaaagtcca
       61 taacgaaaat caggcactca aatgaaaaaa attatgtaaa cagatgaatt ttttgacaaa
      121 tcatcaaatc gaggattcta aaatcgcaag aatatccaaa attctgaaaa aaatatccaa
      181 aattatagat ttctaatcta gggttactga tacagcgttc aattcgccag acacgaacca
      241 gaattccaaa aaaatggatt tcttaattct cccaccagtc taaacctggc aatccttgtc
      301 tctggtgcag acacttgagg tcaccaggtc tccctgtctt caggtttcat ggtatgattt
      361 gctgtcacac actcaagttt aggcccttct ctttcacccg gacacaccat ctttcagcta
      421 ctcatctgag gcggtaatgt ctcttcttga gcaaatattt tttcttcctc aaagactctg
      481 ccggctgtgc tgtcagatca atggcctgtg ggtgatcctc ctggtattgg ggtgggggca
      541 caccggactg gcacttgatc ccgccaaaac actatcccag tacggttatg atgagtggca
      601 ggacggatta ccccaaaaca cggtgcatac cgtggtacag acccaggatg gctatctctg
      661 gcttggcacc tatgaaggac tggtccggtt tgatggcgct cagtttaaag tttttgaccg
      721 gagcaccgtt ccccaaatca aaaacaacag catttgggtc gtgagggaaa gccctgacca
      781 gacattgtgg attggaacac tgggcggggg gttactcagt tatcgcaacc atcaattcgt
      841 gggatatgac acagccgaag gattggtcag cagttttgtt tatgccctct gcccaagtcg
      901 tgatggcggg ctctggattg gaaccaacca gggtctgtgc aagttggacc agggtcagtt
      961 caagtcatat ccagaacaca aagaactcgc tggaaaacta attcgcgccc tgtacgagga
     1021 caaaaacggg cgactctggg tcggcaccga cacccagggg ttaatctgcc tggaggcagg
     1081 tcgaacgaca gtgtactcaa ccgccagcgg gtttccgagc aatagcattt atgcggtgct
     1141 tgaagatgcc cagggtggcc tgtggattgg aacctatggt ggcggcctgt gttacctgcg
     1201 tgacaaccgg tggaccgcct acaccacacg cgatgggttg ccagacaacc tgattcgctc
     1261 gctttattta gatcgccagg gggtcctttg gattggaaca c
//
LOCUS       DAOQDO010002183         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_128862_length_1301_cov_1.992777, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002183 DAOQDO010000000
VERSION     DAOQDO010002183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128862_length_1301_cov_1.992777"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKZ53_25885"
     CDS             <1..612
                     /locus_tag="PKZ53_25885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43939.1"
                     /translation="GQVVLREGADLSQANSGVQLLVELVQSNENYLFFADTNGATAAD
                     LFQGSRDSKGRQTQMGKSLANRFNGSISQTGGVGYLLGTSGRPTQLQPANLANGDPVF
                     SVIAYNTNLVQTQVGIDASSIFPGAAPSPEVRGQMEGMGHIIRPVSFFIHEAAENRKF
                     EEIGPKYETAHHHAIRREGIIRQELRIGGGFAGAVIQSVVKKP"
     gene            636..1094
                     /locus_tag="PKZ53_25890"
     CDS             636..1094
                     /locus_tag="PKZ53_25890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ43940.1"
                     /translation="MRLFVAILLVLWSFSEATGHQCRCKPPEPNETTHPGGNEIITVI
                     EQVPYKRIEGQVQDIGGQALKDVLVEVFDRPEWITKGLVASPVGQKRIKACKTDALGR
                     FCFHNIPAGEYEICASKDSEWNPSHVYVIVQPHNRKATKKKLIIHLTLGS"
BASE COUNT          397 a          250 c          321 g          333 t
ORIGIN      
        1 gggcaagtgg ttctccgtga aggagctgac ttgtcgcagg caaattctgg agttcagtta
       61 cttgttgaat tggttcagtc aaatgaaaac tatcttttct ttgctgacac aaatggagca
      121 actgcggctg atttatttca gggttcaaga gacagtaaag ggcgtcagac acagatgggg
      181 aaatctttgg ccaatcgatt taacgggagc atctcacaaa ctggtggtgt tggttatttg
      241 ctcggcacaa gcggaagacc tactcaatta cagccagcaa atcttgcaaa tggcgatcct
      301 gttttttctg tgatcgctta caacacaaac ttggtgcaga ctcaagttgg aattgacgca
      361 agcagcattt ttcctggggc ggctccatct cccgaggttc gtgggcaaat ggaaggaatg
      421 ggtcacatta ttcgacctgt tagcttcttt atccatgaag ctgctgaaaa ccgaaagttt
      481 gaagaaattg ggccaaaata tgaaacagca caccatcatg ccattcgacg tgaaggtatc
      541 atcaggcaag agcttcgaat cggcggtggg tttgcaggag cggtaataca gtctgtagtt
      601 aaaaaaccat agttcggagg ttggaaaatt aagatatgag actatttgtt gctatcttgc
      661 tagtcctttg gtctttttct gaagctacag gccatcaatg tcgctgtaaa ccacctgagc
      721 caaatgagac aacacaccca ggtggaaatg agataattac tgtcatagaa caagttccat
      781 ataaacgcat tgaaggtcag gtgcaagata ttggtggtca agcattaaaa gatgttttgg
      841 ttgaggtttt tgatagacca gaatggatca ccaaagggct cgttgcatca ccagttggcc
      901 aaaagagaat taaagcctgc aaaacagatg cactcggaag gttttgcttt cacaatattc
      961 cagcaggtga atatgaaata tgtgccagta aagactcaga gtggaaccca tctcatgtgt
     1021 atgtgattgt tcaaccccac aaccgaaaag caacaaagaa aaaattgatt atccatctca
     1081 cacttggtag ctaaattcga attgtaaatg attatttctg taacaaattc tgtggagaag
     1141 cgtcacccag attgaggaaa ccgaaaaccg gcaggacctg gtggtgaatg gacgaaataa
     1201 ccgggtgaac gaaactgctg gaatgagcta tgatgccgcc gggaatgtga tctttgacaa
     1261 tggaaagaca ttcaagtacg acgcggaaaa ccggatggtg g
//
LOCUS       DAOQDO010002184         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_129020_length_1300_cov_2.274699, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002184 DAOQDO010000000
VERSION     DAOQDO010002184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129020_length_1300_cov_2.274699"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKZ53_25895"
     CDS             complement(<1..404)
                     /locus_tag="PKZ53_25895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43941.1"
                     /translation="MELFDYFQIQGFPGKMVRCWNLDDQRSESGVEKMPAYDQSSNEV
                     TFKIVYFGPGLAGKTTNIQYIHRSKAPQSKGKIVAKYKEDLGHILSCEFYPQVSFQGY
                     NVKFHLMARVGNLFYLTGWPYVLQDVDGVVFVA"
     gene            549..1259
                     /locus_tag="PKZ53_25900"
     CDS             549..1259
                     /locus_tag="PKZ53_25900"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNJ43942.1"
                     /translation="MSFLFFLLQSEHDVPANTRWLSERETVIADQLKFPKRRQEWLLG
                     RWTAKHALHAFFQLVEKPEDALQELTAVETVTAEEFAIFEILPTGTGAPAAYLQGAIA
                     PVSLSLSHRAGLAMCLIGLPGTAIGCDLELIEPRSPEFIADYFTPAETSLIISLSEPN
                     RSQTATLIWSAKESALKCLQQGLNVDTRSVEVTLSDSESSPSKWQPFTIRECTTNQRF
                     SGWWRRQGTFLMTICGAI"
BASE COUNT          321 a          330 c          297 g          352 t
ORIGIN      
        1 gctacaaata caactccatc aacatcttgt agaacatatg gccaacccgt taaatagaat
       61 aaattcccga ctctagccat caggtggaat ttgacattgt aaccctgaaa agaaacctga
      121 gggtaaaatt cacaagacaa aatatgcccc aggtcttcct tatattttgc aactattttt
      181 cctttagatt gaggggcttt gctgcgatgg atgtattgaa tattcgtggt tttccctgcc
      241 aatccagggc caaagtagac aattttgaag gtgacttcgt tggatgactg gtcgtaagct
      301 ggcattttct ctactccgct ttcagatcgc tgatcgtcaa gattccagca tcgtaccatt
      361 ttccctggga atccttgtat ttgaaaatag tcaaataatt ccatcaaatg agtttagtga
      421 acggctgact actgaccatt gactactgac tgacataaaa gctaaagctt gaactctgag
      481 cctttcattc ttcaatcaag cccacccact aacgcaggtg gtactgactt actattcttc
      541 attcgcttat gtcttttctt ttctttctgt tgcaatcaga gcacgacgtt ccggccaata
      601 cacgctggct ttcggagcgt gaaacggtca ttgccgacca gttaaaattc cccaagcgcc
      661 gtcaggaatg gctgcttggt cgctggacgg ccaaacatgc gcttcatgca ttctttcaac
      721 tggttgaaaa accagaagat gcgttgcagg aactgacggc ggttgagacc gtaaccgccg
      781 aagaattcgc catctttgaa attcttccaa ccgggacggg tgctccggcg gcatatcttc
      841 agggagcaat tgctccagtc agcctttcgc tcagtcatcg ggcgggactg gcgatgtgtt
      901 tgattggact tcccggaaca gccatcggct gcgacctgga attgatcgaa ccgcgcagtc
      961 ccgaatttat cgcggattat ttcactccgg ctgaaaccag cttgataatt tcattatcag
     1021 aaccaaatcg ctcacaaacc gccaccctca tctggagcgc caaggaaagc gcgctcaaat
     1081 gcctccaaca gggcctcaac gttgacacgc gttcggttga agtcaccctg tccgattctg
     1141 aatcttcacc ttcgaaatgg cagccgttta caattcggga atgtaccaca aatcagcggt
     1201 tctccggctg gtggcgacgg cagggaacgt ttttgatgac aatttgtggc gcgatttagg
     1261 gttccgggtt ccgggttccg ggttccgggt tccgggttcc
//
LOCUS       DAOQDO010002185         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_129734_length_1296_cov_3.978243, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002185 DAOQDO010000000
VERSION     DAOQDO010002185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129734_length_1296_cov_3.978243"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1296)
                     /locus_tag="PKZ53_25905"
     CDS             complement(<1..>1296)
                     /locus_tag="PKZ53_25905"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNJ43943.1"
                     /translation="PPTMWTTLSNPTLKRLARLKPESQRAVVVNTKFDPRAKRTSEPP
                     VAYELTEVTRQALTELARTVEPGGFLLVYGLPHELPLYGLFLSELKDERFSLAFKYWI
                     ALDLDDAPRRETLQPSHLGLLLFHKCKTEGKTPTRFHFNTATVRVPHAFCAACGLNLK
                     DWGGKKHLMNPLGTAMSDIWRDLPKQKLTSHLLPESVLERIAALVHKPKAKLLHVIEG
                     QSGVASSLLLKTKTDPIAIPASEWQTLTRLEPNQVYQGDCVSFLNRVAELYPEGVFDL
                     VFADPPYNLNKPYGECDDGLVEREYLDWCQAWLDGLIRTLKPGGALLTLNLPKWAMYQ
                     AAFLNERLEFRHWIVWDALSEPRGKLMPAHYALLYYTKAGGKGTVNYSPLGKESKPGA
                     VMPPNAPIYCLRPKCVRSRTLDGHDTRVELSDVWSDIQRI"
BASE COUNT          297 a          341 c          353 g          305 t
ORIGIN      
        1 gatgcgttga atatcgctcc acacatcgga cagttcaacg cgggtgtcat ggccgtctaa
       61 ggttcggctg cgtacacatt tgggtcgcaa acaatagatt ggcgcattgg gcggcatcac
      121 ggcgccaggt tttgactctt ttccgagtgg cgaatagttg actgtgccct tcccgccagc
      181 tttggtgtag tagagcaacg cataatgcgc cggcatcagt ttgccgcgag gttcagagag
      241 cgcatcccac acaatccagt gccggaactc aagccgttca ttgagaaatg ccgcctgata
      301 cattgcccat ttgggtaaat tgagcgtcag gagcgctccg cccggtttga gcgttcggat
      361 cagtccgtca agccacgcct gacaccagtc aaggtactca cgctcaacca gaccgtcgtc
      421 acattcgcca taaggtttgt tcaaattgta cggcgggtcg gcaaacacaa ggtcaaacac
      481 cccttccgga tacaattccg ccacacgatt gaggaatgat acgcaatcgc cctgatacac
      541 ctgattgggt tcgagtctgg tgagggtttg ccattcagaa gctggaatag caatcggatc
      601 cgtctttgtt ttcaacaaca gcgaagaggc aacacccgac tgcccttcaa tgacgtgcag
      661 gagtttggct tttggtttgt gaaccagcgc cgcaatacgt tccaataccg attcgggcag
      721 caggtggctg gttaatttct gttttggcag atcacgccag atatcggaca tagccgttcc
      781 aagcggattc atgaggtgct ttttgccacc ccaatctttc aggttcaacc cgcaggcagc
      841 acaaaaagcg tgaggtaccc gaaccgtcgc cgtgttgaag tgaaaccgcg tcggggtctt
      901 accttcggtt ttgcatttat gaaacaggag caaccctaaa tgcgatggtt gcagcgtttc
      961 acggcggggc gcatcatcca gatcaagggc aatccagtat ttgaacgcca gcgaaaaccg
     1021 ttcatccttg agttcagaca ggaacagtcc atacaacggc aattcgtgcg gcaggccata
     1081 caccagcaaa aaaccgccag gttcgaccgt gcgggccagt tcggtcagtg cctgtctggt
     1141 gacttcagtc aactcataag ccacgggcgg ttcgctggtt cgtttggcgc gtggatcaaa
     1201 tttggtattg accaccaccg cccgttggct ttcgggtttg agtctggcaa gtcgtttgag
     1261 tgtcggattg gaaagcgttg tccacatggt aggggg
//
LOCUS       DAOQDO010002186         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_129777_length_1296_cov_2.647865, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002186 DAOQDO010000000
VERSION     DAOQDO010002186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129777_length_1296_cov_2.647865"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1296
                     /locus_tag="PKZ53_25910"
     CDS             <1..>1296
                     /locus_tag="PKZ53_25910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNJ43944.1"
                     /translation="QRGWVKDVEFDFIRKDGSILPVLLSATAIRDEAGNFVMSRSTIF
                     DNTERKEAELAVRESEATYRALFENSNDGIFLMSATGEELQANQQALGMLGYTLEEYL
                     SMGKENQNPFTVEAAQRRDSDEKFAALLNGEKVPLYERVFTAKNGRKVLVEINLSPVR
                     DENGRIIMVQSVVRDITQRKAAEEALHHSRDQLSATNLALQKASQAKNEFLANMSHEL
                     RTPLNAILGMSEILLDEIRGPLNERQHNMVNIIESSGRHLLSLINDILDLSKIEAGKL
                     ELHFEKVSVMDICQTSLIFIKEPAAKKSIQVEFEADPLVSTVYADMRRLKQILINLLN
                     NAVKFTMPNGYVKLKVKSDADNHLIHFSVIDNGIGISVDDQTRLFTPFTQVDSSLTRE
                     YEGTGLGLALVMELVELHNGSVKLESEVGQGSTFTVSIP"
BASE COUNT          350 a          318 c          325 g          303 t
ORIGIN      
        1 ccaacgcggc tgggttaaag atgtggagtt cgatttcatt cgaaaggacg gttccatact
       61 gccggttctg ctgagtgcca ctgcgattcg tgacgaagct ggcaattttg taatgagccg
      121 gtccacgatc ttcgataata ctgagcgcaa agaggcagaa cttgccgtcc gtgaaagtga
      181 agcgacatac cgtgctctat tcgaaaactc aaatgacggt attttcctga tgtccgccac
      241 cggtgaagag ctgcaagcca accagcaggc tctcggtatg cttggctata ccctggaaga
      301 ataccttagc atgggtaagg agaatcaaaa cccatttacc gtggaagcag cccagcgccg
      361 ggattcggat gaaaagtttg ccgccctttt gaacggcgaa aaagttccgc tatatgaacg
      421 tgttttcacg gccaaaaatg ggcgtaaagt tttggtggaa ataaaccttt ccccggtgcg
      481 tgatgaaaac ggcaggatca tcatggtgca aagtgtggtg cgggatatca cccagcgcaa
      541 ggcagccgag gaggcgctgc accatagccg tgaccaactg agcgccacga accttgcctt
      601 gcaaaaagcc tcacaggcaa aaaacgaatt ccttgccaac atgagccacg aattgcgcac
      661 gccgctcaat gctattctgg gcatgtctga gatcttgttg gatgagattc gcggcccact
      721 caatgaacgc cagcacaata tggtcaacat catcgaatca agcgggcggc atctcttaag
      781 cctgatcaat gatatcctcg acctttccaa gatcgaggcc ggaaaattgg agcttcattt
      841 tgaaaaggtc tccgtcatgg atatctgcca gaccagtttg atctttatca aagaaccggc
      901 cgcaaagaaa agcatccagg tcgagtttga agcggatcct ttggttagta cggtttatgc
      961 agatatgcgc aggttgaagc aaatcctcat taacctgttg aacaatgctg tcaaattcac
     1021 aatgcctaat ggttatgtca aactcaaggt taagtctgat gcggacaatc atctgattca
     1081 cttctccgtc atcgacaacg gcattggcat ttcagtcgat gatcaaactc gtctattcac
     1141 accctttact caagtggaca gcagcctgac ccgcgaatat gaaggaaccg gcctgggcct
     1201 ggcattggtc atggaattgg tggaacttca caacggcagt gtcaaattgg aaagtgaagt
     1261 tgggcagggc agcaccttca ccgtttccat cccctg
//
LOCUS       DAOQDO010002187         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_129907_length_1296_cov_1.503626, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002187 DAOQDO010000000
VERSION     DAOQDO010002187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129907_length_1296_cov_1.503626"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKZ53_25915"
     CDS             complement(<1..536)
                     /locus_tag="PKZ53_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HNJ43945.1"
                     /translation="MIDHDRLFKELISTFFVDFLDLFIPETLEYLDVNSLEFLDKEVF
                     TDVTAGDRHEADLVVKGRFQGQPACFLIHVEHQASKKGNFPERMFTYFCRLREKYGLP
                     VYPIVLFSYDEPLIAEPTRFEVRFPDRLILQFDYTGIQLNRLNWREFLVRKNPLASAL
                     VNRPGPHGPNLSRVPTTPS"
     gene            complement(564..1184)
                     /locus_tag="PKZ53_25920"
     CDS             complement(564..1184)
                     /locus_tag="PKZ53_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43946.1"
                     /translation="MVFHLVIIGILFFVVYGVMALFGQGVWWVALTMATVVYLVGRLI
                     IKNALKNLFLAPFKAKGAVLRGAEAQVHSIVRVEAPVKDLVSVLDDAEETAHPRPDRS
                     TWKYYQIDVTINPQSPNGAFSHWEPGELRLLPIHALVNPDNEDEEEISELSKLEIFEE
                     GVFKPDDGYKLPGSRRLRLLTAVHPAHQQVKFAYYFETFGNLTLPN"
BASE COUNT          367 a          319 c          279 g          331 t
ORIGIN      
        1 gatggcgtcg tgggtacacg actcaagttc ggcccgtgag ggccgggtct gttgactaaa
       61 gcgctggcaa gcggattttt tcgtaccagg aattctcgcc agttgagccg gttcaattga
      121 atgccagtat aatcaaattg caggatcaat cgatctggga accgaacttc aaaacgagtc
      181 ggttcagcaa tcagtggctc atcataggaa aacaaaacaa ttgggtacac tggcaagcca
      241 tatttttctc gcagccgaca aaaataggtg aacatccgtt ctggaaaatt ccccttcttt
      301 gaagcctgat gctcaacgtg gatcaaaaaa caagctggct gaccttgaaa tctgcccttt
      361 accaccagat cagcctcgtg acgatcacca gcggtaacat ctgtgaagac ttccttatct
      421 aaaaattcaa gtgagttaac atcaagatat tcaagggttt caggaataaa aagatcaagg
      481 aaatcaacaa aaaacgtgga gatgagttct ttaaacagcc gatcatgatc aatcatagaa
      541 actccaggaa ccagcttttg ggattagttt ggaagtgtca gatttccaaa ggtttcgaaa
      601 taataggcaa atttcacctg ctgatgcgcc gggtgaactg ctgtcagtaa ccgcaatcgc
      661 ctggaaccag gcaatttata tccatcatcc ggtttaaaga ccccttcttc aaagatttca
      721 agctttgaaa gttcactgat ctcctcctca tcctcattgt ctgggttcac cagggcatgg
      781 atgggcaaga gtcgaagttc accaggttcc cagtgtgaaa aggctccgtt tgggctttgc
      841 gggttaatcg ttacatcaat ctggtaatac ttccacgtac tccggtcggg tcgggggtga
      901 gctgtttctt ccgcatcatc taacaccgat accaggtctt tgactggcgc ctcaaccctt
      961 acaattgaat gaacctgcgc ctcagccccg cgcaagacag ctcccttggc tttaaatggt
     1021 gccaggaaaa ggttttttaa cgcgttttta atgatcaatc gcccaaccag atatacaacc
     1081 gttgccatcg ttaacgccac ccaccacacg ccctggccaa aaagggccat cacgccgtac
     1141 accacaaaaa acagaattcc aatgatcaca agatgaaaaa ccacagcctg tgcctccaag
     1201 aattgaattg atgtgcgggg ctctgtttat tacaaggcaa tataccttaa ttttttctat
     1261 ctgcaagcat tttcctggct catgatctgc ctgttc
//
LOCUS       DAOQDO010002188         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130026_length_1295_cov_2.141129, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002188 DAOQDO010000000
VERSION     DAOQDO010002188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130026_length_1295_cov_2.141129"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PKZ53_25925"
     CDS             complement(<1..1206)
                     /locus_tag="PKZ53_25925"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ43947.1"
                     /translation="MSVSLVAVVSGQRYGRPTPAPGYFDASESAKLEFRLSEGTGHPA
                     AIPAPPRAVSQSLSRSETQTILDRLPVLDPLRKTDPSFVLPVTTRRPPKGGDVVKTVL
                     APSVPVQPAPPVPEPVDTSPVEIVRYQPETYIGDAPVVSVTFSQPMVSVTSVRTLTQA
                     ESPVKITPEVPGTWQWLGTQTIAFNPATRFPKATRFDVTVDPAIRSALNNPLKEGKNW
                     SFSTPAPRVVQFAPSGTVGVEPVMVVTFNQEIDPAQVVSLTQVRVADQELPVRMATEA
                     EIQVDDYVSSTVKIAGSNRCVAFRALNPFPKSSLIILQIQPGVRSKEGPLVSVKSNYF
                     SFSTFAPLKILETSCRTYSGCRPNSDWIITFSNPLDTASFKPEFISISPPISKPEIED
                     YRQKYQVISS"
BASE COUNT          319 a          300 c          369 g          307 t
ORIGIN      
        1 gctggaaatg acttgatatt tttggcggta gtcttcaatt tcaggttttg aaattggagg
       61 actgattgaa atgaattcgg gtttaaatga ggccgtgtca agcggattgg aaaaggtgat
      121 gatccagtcg gagttgggac gacatcccga ataggtgcgg caactggttt ctagaatttt
      181 cagaggcgca aaggttgaaa aggaaaagta atttgatttt acggaaacca gcggcccttc
      241 ttttgatctc actccgggtt gtatttggag aataatcagc gagcttttcg gaaatgggtt
      301 gagggctcga aaggccacac accgattgct accagctatt ttgacagtgc ttgaaacata
      361 gtcatccacc tgaatttcag cctcagtggc cattcggacg ggcagttcct gatcggcaac
      421 tctcacttga gttaacgata ccacctgggc tggatcaatt tcctgattaa atgttacaac
      481 catcacgggt tccaccccaa cggtgccgga tggagcaaat tgcaccacac gtggtgccgg
      541 ggtcgaaaag gaccagttct ttccttcttt gagcggattg ttgagggctg agcggatagc
      601 tggatcaacc gtgacgtcaa accttgtggc ctttgggaac ctggtcgctg ggttaaaagc
      661 aatcgtttga gttcccaacc actgccaggt gccgggtact tcgggggtga ttttgactgg
      721 ggattcagct tgcgtcaggg tacggacact cgtcaccgac accatcggct gtgaaaaggt
      781 cactgaaacc acgggtgcgt caccgatata cgtctctggt tgataccgaa caatctcaac
      841 cgggctggta tcaaccggtt caggtactgg cggcgcgggt tgaactggaa cggaaggagc
      901 caaaaccgtt ttgaccacgt ctccaccttt gggaggtctt cgggtcgtga cgggcagaac
      961 aaacgacggg tcggtttttc ggagcggatc cagcaccggc aggcgatcta aaatggtttg
     1021 ggtttcagac cgcgaaagcg actgcgatac ggcacgagga ggtgctggaa ttgctgcagg
     1081 gtgtcccgtt ccttcactga gtcgaaattc aagtttggcc gattcggaag cgtcaaaata
     1141 cccaggtgca ggtgttggtc gtccatatcg ctggcccgac accacggcga ccagtgagac
     1201 agacacaacc agcagtccca gaaaacctgt caatcgagtc aggccaaaac cagcgttgcc
     1261 gaagacaaaa aggctacgga tgctcatatg agttt
//
LOCUS       DAOQDO010002189         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130048_length_1295_cov_1.866129, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002189 DAOQDO010000000
VERSION     DAOQDO010002189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130048_length_1295_cov_1.866129"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="PKZ53_25930"
     CDS             <1..>1295
                     /locus_tag="PKZ53_25930"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ43948.1"
                     /translation="SLQNGKPIENTTVTLFPDGVESTSDAQGVATFQRPASSSLNSKK
                     PGFLVARQGNDCAILPKSLYLRGDVTNWTRSDQGNSLVWYVYDDRHLYRPGETLHLKG
                     WIRVADEHSTGDLLPPGQPPQTITYTLKDFQETELVTGSTQTNAYGGFDLELQLPETI
                     STGQTWIEFKTIPTFSPINEQTCTFWHAFQIEEFRRPEFVTSVTSDRKACLIGESAEV
                     TVAANYFAGGPLPGATVNWSVSTQPGTYAPPNWDQFTFGTWIPWWEYQGETTEENKMR
                     LPGKTDLSGKHQIHLDFQSVNPPRPMTVFAEASIADVNRQSSSDLSVLLVHPSSLYVG
                     LKPDRTFVELNHPFSFETIVTDIDGKPQPDQAVSFEVVWLESVFRNKEWAEVEHPVQT
                     GNLISELTPALWQFTPAKGGRYRITARVVDAKGRPNQTQ"
BASE COUNT          338 a          335 c          308 g          314 t
ORIGIN      
        1 tcgctccaga atgggaaacc cattgaaaat acaaccgtaa cgctctttcc tgacggagtt
       61 gagtcaacct ctgatgctca aggagttgct acctttcaac gtccagcttc gtcatctctc
      121 aactcaaaaa aaccagggtt tttagttgct cgccagggaa atgactgcgc catcttacct
      181 aaatccctgt acctcagggg tgatgtaacc aattggaccc gttcagatca gggaaattca
      241 ctggtctggt atgtgtatga tgaccggcat ctctaccgac caggagaaac acttcatctc
      301 aaaggctgga ttcgagtggc tgatgaacat tccacaggtg accttctccc acctggccag
      361 cccccccaaa cgattaccta cacgctaaaa gattttcagg aaacagaact ggttacgggt
      421 tcaactcaaa cgaacgctta tggtggattt gatctggaac ttcagcttcc agaaacaatt
      481 tccaccgggc aaacctggat tgaatttaaa acgatcccaa cattctcacc aatcaatgaa
      541 cagacatgca cgttctggca cgccttccag atcgaagagt ttcggcgacc ggaatttgtc
      601 accagcgtga ccagtgaccg caaagcctgt ctgattggtg aatcggctga agttaccgtg
      661 gcggccaatt attttgctgg tggcccctta cctggcgcca cagtgaattg gagcgtgagt
      721 acccagcctg ggacatacgc ccctcccaac tgggatcaat tcacatttgg tacctggatt
      781 ccgtggtggg aatatcaggg tgaaaccact gaagagaaca aaatgaggtt gcccggaaag
      841 accgatctat cagggaaaca tcagattcac cttgattttc agtcagtcaa tcccccacgc
      901 ccgatgactg tctttgccga agcgtcaatt gcggatgtca accgacaatc atccagcgat
      961 ttatcagttc ttctggtgca tccgtcgagt ttgtacgtcg ggttaaaacc ggaccggacg
     1021 tttgttgaac tcaaccatcc attttccttt gaaaccatcg tgacggatat tgacggcaag
     1081 ccacaaccgg accaggccgt ttcgtttgag gtagtttggc ttgagtcagt ctttagaaat
     1141 aaggaatggg ctgaggttga gcacccggtt cagacaggta atctgatttc ggaattgaca
     1201 cctgcactgt ggcagtttac cccggcgaaa ggcggtcggt atcgaattac agcacgggtt
     1261 gttgatgcca aggggcgccc caatcaaacc cagat
//
LOCUS       DAOQDO010002190         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130117_length_1294_cov_3.367232, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002190 DAOQDO010000000
VERSION     DAOQDO010002190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130117_length_1294_cov_3.367232"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1294)
                     /locus_tag="PKZ53_25935"
     CDS             complement(<1..>1294)
                     /locus_tag="PKZ53_25935"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNJ43949.1"
                     /translation="LVNIFGHLGPAFIKLAQILAVREDLLPKLYAREFSRLLDQTPAA
                     NWSYVEATVRRNTGKAPSDIFESFQTTPIASASIGQVHRARYQNRDVVVKIRRPQVVE
                     TILLDTSTLSILMEWIRPIFGSHYLYRGFEVLLSEFKRIVVAELDFRIEATNADRIRA
                     QEPRHPRLIIPEMIPELVYEDLLVMEFCEGVRIDQIETIRTYGVDLNELIETLLEIIF
                     SQLLVHGFFHADPHPGNLLINRQGQIILLDYGMMDELDPVTRDDFLGLILAANNNDYE
                     EVTRKLYLLGMVATDAPTDDLRMVTETILNLRFLVRTHQRQVQQAVEELFEKTRILHH
                     LRMPRQMVYLMRMGTLIEGVAIRFDDNFDSIRDAVPIAKRVGLRIIARIVPFATALRY
                     TAEVVAEQVDAFLGRLTQRKKVTVVRNFVSDVTRRLRPA"
BASE COUNT          323 a          311 c          366 g          294 t
ORIGIN      
        1 gctgggcgga ggcggcgcgt gacgtcgctg acaaaattgc ggaccacggt gacttttttg
       61 cgctgggtaa ggcgaccgag gaaggcgtca acctgttcgg cgacgacttc agccgtgtag
      121 cggagcgccg tggcaaaggg aacgatccgg gcaatgatgc gcaggccgac tcgtttggca
      181 atcggaacgg catcccgaat gctgtcaaag ttgtcgtcaa aacgaatggc cacgccttca
      241 atcaacgttc ccatccgcat cagatacacc atctggcgtg gcattcggag atggtggagg
      301 atccgggttt tttcaaaaag ttcttcaact gcctgctgaa cctgacgctg gtgagtgcgc
      361 accagaaatc gaaggttcaa aattgtctcg gtgaccattc gcaaatcatc agttggggca
      421 tcggtggcca ccatcccgag caggtacagt ttgcgggtga cttcttcata gtcgttgttg
      481 ttggctgcca aaattagccc cagaaaatca tcgcgtgtca ccggatccaa ttcgtccatc
      541 atgccatagt cgagcaggat aatctgacct tgccgattga tgagcaagtt tccaggatga
      601 gggtcggcgt gaaaaaagcc gtggaccagc aactgggaaa aaatgatttc aagcaaagtt
      661 tcaatcagct cgtttaaatc cacgccatag gtgcgaatgg tctcgatctg gtcaatgcga
      721 actccttcgc aaaattccat caccagtaaa tcttcataca cgagttctgg aatcatctcc
      781 gggatgatca gccgtggatg gcgaggttcc tgggctcgaa tccggtcggc attggtggct
      841 tcgatccgga aatcaagttc agcgaccact atgcgcttaa attcactcag taacacttca
      901 aacccacgat acagataatg cgatccaaag atgggccgaa tccactccat caaaattgag
      961 agggttgagg tgtcaagtag aatggtttca accacctgtg gccgacggat tttgaccacg
     1021 acgtcacggt tttgatatct ggcgcgatgg acctgcccaa ttgaagcgga ggcaatcggt
     1081 gtggtttgaa atgattcaaa aatatcggat ggagcttttc cagtgtttcg acggacggtg
     1141 gcttcgacat agctccagtt ggcggctggg gtttgatcga gcaggcggga aaattcacgg
     1201 gcgtagagtt tggggaggag gtcttcacga acggcgagaa tctgagccag cttaataaag
     1261 gccggaccca ggtggccgaa gatattgacc agcc
//
LOCUS       DAOQDO010002191         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130127_length_1294_cov_3.026634, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002191 DAOQDO010000000
VERSION     DAOQDO010002191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130127_length_1294_cov_3.026634"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PKZ53_25940"
     CDS             complement(<1..1169)
                     /locus_tag="PKZ53_25940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43950.1"
                     /translation="MNPIKRRSGLSIKLPDAFKLLEAELQRDESLISDILPELPELPE
                     APELSDLPELPEAPKLVLDTLEEFKGRDKLIQSLVKKGFQPIDQLELEPVSLLPPEGL
                     KLVGRPAKRQTFKLSLPVLPSEHAVVLLEQDGVYTWNFPRPTPPVVPKSAESEGESSL
                     QGEEAPEEKVLDFEFSLESEPEPTAGEQPKIKGLVGDFLADKIKAYVLKFAGDWAVGK
                     TAEFLERDIQQGLISMPSSTLEDWKLGVDLSSVASRSDRPARILLFIHGTFSSTKGAF
                     GALTASDFGRQFLETALAKYDAVIGFDHKTLSVDPKVNATDLLTALSQYPWNQPPQFD
                     VVTHSRGGLVFRSLAELVLPGSGWKASFDHVVFVAAANGGTLMAEPDNWHYLVDL"
BASE COUNT          314 a          322 c          333 g          325 t
ORIGIN      
        1 tagagatcaa ccagatagtg ccagttgtcg ggctcggcca tcagtgttcc gccattggct
       61 gccgcaacaa acacgacgtg gtcaaaactg gctttccagc ctgacccagg caagaccagt
      121 tcggcgagac tgcgaaacac cagtccgcca cgactatgcg tgaccacatc aaattgtgga
      181 ggctgattcc acgggtactg gctcagcgca gtgagcaaat cagtggcgtt tacctttggg
      241 tctacgctca aggtcttgtg gtcaaaccca atcacagcat catatttcgc cagggccgtt
      301 tccaaaaact gcctgccaaa atcgctggcg gtgagtgcgc caaaggcacc tttggtgctg
      361 ctaaacgtgc cgtggataaa gagcaaaatt ctggccggac gatcagaccg gctggcaact
      421 gaggaaagat caaccccgag tttccagtct tccaacgtgc ttgacggcat cgaaatcaat
      481 ccttgctgga tatcacgttc caaaaattca gccgtctttc ccaccgccca gtcaccggca
      541 aatttcagca cataggcttt gattttatcc gcgagaaaat caccgaccag ccctttgatt
      601 tttggctgtt cacctgcggt tggttccggt tcagattcca ggctaaattc aaaatcaagg
      661 accttttctt cgggggcttc ttcaccctgg agactggatt cgccctcaga ttcggcactt
      721 tttggaacca caggcggtgt tgggcgagga aagttccagg tatacacgcc atcttgttcc
      781 agcaacacaa ccgcatgctc agaaggcaac accggaaggc tcagtttgaa cgtctgacgt
      841 ttggctggcc ttccaaccag tttcagtcct tcaggtggca gcagggacac cggctctaat
      901 tcaagctgat caatcggctg aaatcctttt ttcaccagcg attgaatcag tttatcccgg
      961 cctttgaatt cttcgagtgt gtcaagaacc agctttggag cttcgggcag ctctggcaaa
     1021 tcagaaagtt caggtgcttc aggaagttcg ggtagctcag gaaggatatc acttatcagt
     1081 gattcatcac gttgtagctc ggcttcaagg agcttaaagg catccggaag cttgattgac
     1141 aggccgctcc gtcgcttgat gggattcatt gggcagtatc cttttgacga ttgggtgaag
     1201 ggaattttta agttagaaaa ctgacagggg ttgttggaaa agtatgtgct atcttagttg
     1261 gagttttttg agagggaaag gagaagttta tgcg
//
LOCUS       DAOQDO010002192         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130134_length_1294_cov_2.927361, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002192 DAOQDO010000000
VERSION     DAOQDO010002192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130134_length_1294_cov_2.927361"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PKZ53_25945"
     CDS             complement(<1..1273)
                     /locus_tag="PKZ53_25945"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNJ43951.1"
                     /translation="MTDLDGKPQPAQSVTFQVVWLETVYKNKEWTVVGHPVQTDNLIS
                     ELSPALWQFTPAKGGRYQITAQVVDAQGRPNETQMTLWVAGGAIQRKKGTEEQTLELI
                     PNQKEYQPGETAEILVQSPFVPAQGILTLERSGIVTTERFVMTESSHVLQIPIKETYL
                     PNLHATVYLVGESIREDKDGNPLPHLPTMPVSASGTINLLIPPRVRTLSVTATPAQEQ
                     VAPGDETQIAVEVRDAGGNPVDKSECAVVVVDEAILSLTQHIFEHPNDTFYRERQAET
                     GRQHLRNSIGLADESDFIAFNSLKIAGIDHNVVESQVQSLPLFSRNFVQTTTSSLQTT
                     HNGLRASNLIELKPGATSSPSPQQSIRLRANFDPLAVFSPSVVTDANGKAIVSVKMPD
                     NLTKYRVIAFATDGAKRFGKGESSITARLPLM"
BASE COUNT          305 a          315 c          333 g          341 t
ORIGIN      
        1 ccatcaatgg aagccgcgcc gtgatgcttg attcaccttt gccaaaccgt ttcgcgccat
       61 ccgtggcgaa ggcaatcacg cgatatttgg tcaaattgtc aggcattttg actgaaacga
      121 tggccttacc gttcgcatca gtcacgactg aaggtgaaaa caccgccagc gggtcaaagt
      181 tggctctcaa tcggatggat tgctgaggag acggtgagct ggttgctcca ggtttgagtt
      241 cgatcagatt actagctcgt aacccattgt gggttgtttg aagtgaggac gttgttgttt
      301 gaacaaaatt gcgggagaaa agtggtaaag actgaacctg ggattcaacc acgttatgat
      361 ctatcccagc tattttcaaa gagttgaacg caataaaatc tgattcatcc gccagcccaa
      421 ttgaatttcg cagatgctgg cgtcctgttt cggcctggcg ttcgcgatag aatgtgtcgt
      481 ttgggtgctc aaatatgtgc tgagtgagtg aaagaattgc ttcatcaacc acaaccacgg
      541 cacactcact cttatcaacc ggatttccac ctgcatcccg aacttcaacc gcaatctggg
      601 tttcatcgcc aggagctacc tgttcctggg ctggggtggc agttacagac agcgtgcgaa
      661 cacgaggcgg aatcaaaaga ttgatcgtgc cagaagctga aaccggcatc gtcggcaagt
      721 gtggcagcgg atttccgtct ttgtcttcgc ggattgattc accaaccaga taaacagtcg
      781 catgcaggtt tgggaggtat gtttctttga ttggaatctg cagcacgtgg gaagattcag
      841 tcatgacaaa ccgctcagtt gttacaattc cagagcgttc cagcgtcaga atcccctgtg
      901 ccggtacaaa aggagattgc accaggatct cagccgtttc acccggctgg tattcttttt
      961 gattgggaat caattcgagg gtttgttctt ccgttccttt tttccgttga atggcaccac
     1021 ccgcaaccca gagcgtcatc tgggtttcat tggggcgccc ctgggcatcc acaacctggg
     1081 ctgtaatttg ataccgacct cctttcgccg gggtaaactg ccagagcgca gggctcaatt
     1141 ccgaaatcag gttgtctgtc tgaaccgggt gtccaaccac ggtccattct ttgtttttgt
     1201 ataccgtctc aagccatacc acttgaaatg taaccgactg agcgggctga ggttttccat
     1261 caaggtcagt cacgatggtt tcgaatgaaa atgg
//
LOCUS       DAOQDO010002193         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130272_length_1294_cov_1.564972, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002193 DAOQDO010000000
VERSION     DAOQDO010002193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130272_length_1294_cov_1.564972"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PKZ53_25950"
     CDS             complement(<1..912)
                     /locus_tag="PKZ53_25950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43952.1"
                     /translation="MKTYFSIPLLSTLWLLAAPVQSAPPSADDFLPPVQASTPEQQQQ
                     LEQVKGPVQEVKDPATGQTAAEGKTAQDAINFVINKHSVGAEMVKFGSGMGWVATGAG
                     SYEVMENPTATRIAKRNAYVKAFLDAKKKLAEALNGLSTEGRTQVVEQMEQVTDTKAD
                     LANFAATQDEKLEQAVQMLLKGFVIYAVEDDAAKKMVYVSIVTTPKTRGQFNRPSPNS
                     MEADSIRDGLSQVLLEIQNGLIPPVGGKIIQVPATGEIAFVGFGSDVIRSSDNATMQL
                     KMRLNAEKIAQMRAKDALVGMIIGDDTR"
     gene            complement(1029..>1294)
                     /locus_tag="PKZ53_25955"
     CDS             complement(1029..>1294)
                     /locus_tag="PKZ53_25955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43953.1"
                     /translation="VTIDLLDENNQLIASNEVTTNNCQFCAIAYGSPDSYYLVAPFFD
                     ISGPRLRSVAFYFLRERAFQVKVSTEDLPRVKSVKLEFKPAAG"
BASE COUNT          272 a          316 c          364 g          342 t
ORIGIN      
        1 acgggtgtcg tcaccgatga tcatgccgac gagggcatcc ttggcgcgca tttgggcgat
       61 tttctcggcg ttgaggcgca ttttcagttg cattgtggcg ttgtcgctgc tgcggatcac
      121 gtcggagccg aagccgacga aggcgatttc gccggtggcc ggaacctgaa tgattttgcc
      181 gccgaccggg ggaatcaggc cgttctgaat ttccagcagc acttgactca agccgtcgcg
      241 gatggagtcg gcttccatgc tgttgggcga ggggcggttg aattggccgc gcgtcttggg
      301 tgtggtgacg atgctgacat aaaccatctt tttggcggca tcgtcttcga cggcgtaaat
      361 cacaaatcct ttcagcagca tttgcaccgc ttgttccagt ttttcatcct gtgtggcggc
      421 aaagttggcg aggtcggctt tggtgtccgt gacctgctcc atttgttcca cgacctgggt
      481 gcggccttcg gtgctgaggc cattcagggc ttctgccagc tttttcttgg catccagaaa
      541 ggctttgacg taggcgttgc gcttggcgat gcgggtggcg gtggggtttt ccatcacctc
      601 gtaactgccc gcgccagtgg cgacccagcc catgccggag ccgaatttca ccatttccgc
      661 gcctactgaa tgcttattga tgacaaagtt gatggcgtcc tgagcagtct tgccttcggc
      721 ggcggtttgg ccggtggcgg ggtctttgac ttcctgcacc gggcctttga cttgttcgag
      781 ttgctgttgc tgttcggggg tgctggcctg taccggcggc agaaaatcat ccgccgaagg
      841 cggggcggat tggacgggag cggctaagag ccaaagagtt gataacaggg gtatactgaa
      901 ataagtcttc atattcattc cttgctaatt gtgtttgtac caatggatcg attcaatcgg
      961 atgattgccc ctgacccggt agacctgaaa atcgttttca tcaacagtca aaatactgcg
     1021 ctcgcccgtt atccagcagc gggtttgaat tccagcttga cgctctttac acggggtaaa
     1081 tcttcagtag aaactttaac ttgaaatgct ctttccctca aaaaataaaa agccactgac
     1141 cttaaccttg gaccggatat gtcaaagaaa ggtgcaacca aataataaga atcaggagaa
     1201 ccatatgcaa tagcacagaa ctgacaatta ttagtagtta cttcgttgga tgcaattaac
     1261 tgattatttt catcaagaag atcaatggta accc
//
LOCUS       DAOQDO010002194         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130276_length_1294_cov_1.523002, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002194 DAOQDO010000000
VERSION     DAOQDO010002194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130276_length_1294_cov_1.523002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PKZ53_25960"
     CDS             <1..1151
                     /locus_tag="PKZ53_25960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43954.1"
                     /translation="TEMLRVWHPPATLPASSLVQIGKMMDELATRTQIRLVKDPILES
                     PTYTLNWTTSGWTLSGMDGTPQPLGTDLTIDVLKSALAAKNLQAQRVFINLPPSIEFS
                     NRMQLGQGRRFNTVKITPDPVSAQYVLLGRWHNQELQYTWALPNTNPSQKTLTSLPVI
                     TDWIACGTSAESQQKAIEQLEQFALRIGKIRAWLTLESPPDSVLFPYHIALKNDQTGE
                     VNRQGIVRKDEIYRFLLQAHEGIQNSKLVETRYVYLFLIDRNGNTVLLFPRPDLGNVE
                     NLVPYRTTSTALPPKEFLLGNKDDAVTIGEPFGTDTYVFLSSVVPLENPGMLESEGVR
                     IERATKPYSNPLEYLLANLGTATRGSTPTPLISDWSIERITLQSVDAQ"
BASE COUNT          355 a          341 c          295 g          303 t
ORIGIN      
        1 caactgaaat gctccgggtt tggcatccac cagcaacgtt accagccagt tcgctggtcc
       61 agattgggaa aatgatggat gagcttgcca ccaggaccca gattcgcctc gtcaaagatc
      121 caattcttga aagtccaact tacaccctca actggaccac cagtggatgg accttatccg
      181 gaatggatgg aacccctcaa cctcttggca ctgatctgac gatagacgta ctcaaatcgg
      241 ccctggctgc aaaaaatcta caagcccaac gagtgtttat caatctgccg cccagcattg
      301 aattctcaaa ccggatgcaa ctgggacagg gccgccgttt caacacggta aaaatcacac
      361 ctgatcctgt ctcagcccag tatgtcctgc ttgggcgatg gcacaaccag gaactccaat
      421 acacctgggc attgccaaac accaatccgt cccaaaaaac cctcacctca ttaccggtca
      481 ttactgattg gatagcatgt ggaacctcgg ctgaatcaca gcaaaaagct attgagcaac
      541 tcgaacagtt tgccctccga attgggaaaa ttcgtgcctg gctgaccctg gaatcccccc
      601 ctgattcggt cttgttcccg taccacatcg cactcaaaaa tgatcagact ggtgaagtca
      661 accgccaggg aattgttcga aaagatgaga tttatcgctt tcttcttcaa gcacatgaag
      721 gaattcaaaa tagcaaactt gtcgaaaccc gatatgtata cctgtttctg atcgaccgga
      781 atggaaacac cgttttgttg tttccaaggc cggatttagg aaatgtggaa aatttggttc
      841 cctatcggac aacctccaca gcgcttccgc caaaggagtt tcttctgggt aacaaagacg
      901 atgccgtaac catcggtgag ccttttggga ccgataccta tgttttttta agctcggtgg
      961 tgccgcttga aaaccctggg atgctggaaa gtgagggggt tcggatagaa cgggcaacaa
     1021 agccctactc aaatcccctg gaatacctcc tggccaacct gggcactgct acgaggggga
     1081 gtacgccaac tccactcatc tctgattggt caatcgagcg gatcaccttg caaagtgttg
     1141 atgctcaata aagcgaatga agaactgaac gaagagccgt tttcaacctg tgacgtgagc
     1201 accagtggtc ataccaattg gggttcaggg tatcgggttc agggttcagg aaaatacaat
     1261 tttttcaatc atttagccct cccataatac gagg
//
LOCUS       DAOQDO010002195         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130322_length_1293_cov_3.223748, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002195 DAOQDO010000000
VERSION     DAOQDO010002195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130322_length_1293_cov_3.223748"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PKZ53_25965"
     CDS             complement(<1..1102)
                     /locus_tag="PKZ53_25965"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43955.1"
                     /translation="MMNRFVRGSWLVCGIVLIGWILTELSEVPSVFGFSQPVPFTQAQ
                     ELVSGQPIRVDFKGGEKKVFQLRLQVNQYLDLVVDQQGIDVVVRLKSPDGTLQFEADS
                     PNGTRGLEEIWFVSGTAGEYQVEVESTEPSAAPGQFEVTVKTLRSATTTDRVEAEINW
                     TLHQIQVLFASNRIPDALKTAETAIKHGEASLPPDHQLIALAYQELGNLHQVMGKPKE
                     AETATLKALAMFEKTLGPTHEDLVVPLNLLGLLADGAGNFAKAETYLSRAMEIVDHNP
                     NIPEANRATTWNNVALVNDHKGNVQQAEVMYLKALASFEKVNGVDSLNCAPVLNNLGL
                     LYHQQGAFLKAEQYYARSIAIRKKYLAPGHPSI"
BASE COUNT          313 a          328 c          315 g          337 t
ORIGIN      
        1 caatgctcgg atgcccaggt gccaggtatt tcttgcggat ggcaatgctc cgggcatagt
       61 attgttcagc tttgaggaaa gccccttgct gatgatacag cagccccaga ttgtttaata
      121 ccggtgcgca gttcaaacta tcaactccgt ttactttttc aaagcttgcc agcgctttga
      181 gatacatcac ctctgcctgt tgcacattgc ctttatgatc attgaccagc gccacattat
      241 tccaggtggt tgcccgattg gcttcgggaa tgttcggatt gtggtcaaca atttccatcg
      301 cccgtgacag gtaggtttca gctttggcaa aatttcccgc cccatcggcc agcaacccca
      361 gcaggtttaa gggcacaacc agatcctcat gggttgggcc aagcgtcttt tcaaacatgg
      421 ccagggcttt gagcgtagcg gtctcggctt ccttgggctt gcccataacc tggtgaagat
      481 tcccaagttc ctggtaggcc agagcaatta actgatgatc tgggggtaaa ctagcttcgc
      541 catgtttgat ggccgtttca gccgttttca gcgcatccgg gatccgattc gaggcaaaga
      601 gcacctggat ttgatgtaac gtccaattga tttcggcttc aacccgatcc gtagtggtcg
      661 cacttcggag cgttttcacc gtgacttcaa actggccagg ggcggctgag ggttcagttg
      721 attcaacttc aacctggtat tcaccggctg tgccactgac aaaccagatt tcctctaacc
      781 ccctggtacc attgggacta tctgcctcaa actggagtgt gccatcaggg cttttaagcc
      841 gaaccacaac gtctattccc tgctggtcaa ccaccagatc caggtactgg ttgacctgaa
      901 gtcgaagctg aaagactttc ttttcaccgc ccttgaaatc aacacggatc ggttgacctg
      961 aaaccagttc ttgagcttgc gtgaaaggca caggttggga aaaaccaaaa acggaaggaa
     1021 cttcactcaa ttcagtgagg atccatccaa tcaacacaat tccacagaca agccacgagc
     1081 cacgaacaaa acggttcatc atgatctttg aggggtcact tgagagttga gattcaggca
     1141 ggcaagacgt gacgtgtgga gtatgcccga aatgaagccg gagattcaat tggcgggata
     1201 aatcctttgc tcctgataaa tcattgattt ttttggaaca tatccctggt agtcggagtc
     1261 taatttccca ttgcaagaga gtgagatttc cat
//
LOCUS       DAOQDO010002196         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130333_length_1293_cov_2.978998, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002196 DAOQDO010000000
VERSION     DAOQDO010002196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130333_length_1293_cov_2.978998"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..>1293)
                     /locus_tag="PKZ53_25970"
     CDS             complement(203..>1293)
                     /locus_tag="PKZ53_25970"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ43956.1"
                     /translation="ALDVVAPADAEFPASSSTNDQVDEAQAEPFDELPTEDPAWITDS
                     IPVLEISDQADAPVIAPQIEPVLDIPSRTFEFQASAPPAETSPSEAEDQIPASLLLTV
                     EEPATIEMGSARPAQIADETPVVEVHAETTPEVSEPQDVVPENQSEAEPDGFEMPDTL
                     PGVLADVACDEAEPASGPSLEDLIADIDRQAATAPAPSIKPQIRATQYEPATARKRYV
                     VFSLAGSKYAIPITNVIEMGRIPPITAVPYLPEWVRGVTNLRGDILSMIELRAFFGLP
                     PLEQSGAGRMLVVRSQSRGVTTGLIVDGVHGMRELDPDSLKPVTAAIDDQILKFLSGL
                     SVEEDGLLIVLDLESVLSASELRIAHHA"
BASE COUNT          301 a          336 c          340 g          316 t
ORIGIN      
        1 cccataaatg cgaccagcaa gcccagcttt tgccagattt tgagatttcg aagaaaccgt
       61 aacattgtaa atcctcctca atgcgaacca aattatgaat gatgagtttt gaattatgaa
      121 aagactaagc cattattttt caacgaaata atccttcata atttgattaa tccgtcgtaa
      181 gacaaagccg gaaagttaaa cttcaggcat ggtgcgcgat gcgcagttcc gacgctgaca
      241 ggacactctc caaatcgagc acgatcagca acccgtcttc ttcaaccgat aacccggaaa
      301 gaaatttgag gatctggtcg tcaatcgcag ccgtgacggg tttgagcgaa tcgggatcca
      361 gctctcgcat tccgtggacg ccgtcaacga tgagaccggt cgttactccg cgcgactggc
      421 tgcgaacaac cagcatccga cctgcccctg attgttccag tggcggcaac ccaaagaagg
      481 cgcgtaactc gatcatggaa agaatgtcac cgcgtaagtt cgttacccct cggacccatt
      541 ccgggaggta tggaacggcg gtaattggcg gaattcggcc catttcgatc acattggtga
      601 ttggaatggc atattttgag ccagccaatg aaaagaccac atagcgtttc ctggccgtgg
      661 ctggttcata ttgggtggct cgaatctgtg gcttgatgga tggtgctggt gctgtggctg
      721 cctgacggtc aatgtcagca atcagatctt caagtgacgg tcctgacgct ggttcagctt
      781 catcacaggc aacatcagcc aggactccgg gcagggtatc tggcatttca aagccgtcag
      841 gttcagcctc actttgattt tccggcacca catcctgggg ctcgctgacc tctggtgtcg
      901 tctctgcgtg aacctccacg actggtgttt catcagcaat ctgtgctggc ctggcgctgc
      961 ccatttcaat cgtggcaggt tcttctaccg tcagcaaaag ggaagcgggt atctggtctt
     1021 cagcctcaga tggtgaggtt tctgccggtg gtgccgacgc ctgaaactca aaggttctgg
     1081 atggaatatc gagaactggt tcgatctggg gtgcgatcac tggagcatct gcctggtctg
     1141 aaatttccaa aaccgggatg ctgtccgtaa tccacgccgg gtcctcggtt ggtaattcat
     1201 cgaagggttc agcttgagcc tcatccacct gatcattggt tgaggaactg gcggggaatt
     1261 ccgcatccgc tggcgccacc acgtccaaag cct
//
LOCUS       DAOQDO010002197         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130339_length_1293_cov_2.891761, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002197 DAOQDO010000000
VERSION     DAOQDO010002197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130339_length_1293_cov_2.891761"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="queA"
                     /locus_tag="PKZ53_25975"
     CDS             complement(<1..930)
                     /gene="queA"
                     /locus_tag="PKZ53_25975"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNJ43957.1"
                     /translation="MKLSELHFDLPPEQIAQEPCQRRDASRMLKLDRQTGTFSDHQFF
                     EIANFLRPGDVLVLNNTRVFPARLLGHREGFTGRVEVFLVREQGPNLWLSLVKPGRRI
                     PVGTKLTFADGRLRGEVVERTPDGRRIIQFQSEEPFHTVVDAIGQTPLPPYIHRYASG
                     DVRLDRDTYQTVYARERGAIAAPTAGLHFTPELLQRLRDNGVEIVELTLHVGYGTFQP
                     IQTETIEEHHLEGEYFEIPQTTADRVNAAKREGRRVIAVGTTSTRTLETAATVTDDGV
                     ELSAGNGMSELLIYPGYEFKILDGLITNFHLPNS"
BASE COUNT          340 a          302 c          352 g          299 t
ORIGIN      
        1 tgaattgggc agatgaaaat tagtaattaa gccgtcaagg attttgaatt cataccctgg
       61 gtaaatcagg agttcggaca ttccattgcc ggcactgagt tcaacgccat catcagtgac
      121 tgtggctgcc gtttccaggg tgcgggttga ggtggtgcca accgcgatga cccggcggcc
      181 ttcacgtttg gccgcgttga cccggtcagc cgtcgtttgg ggaatctcaa aatactcccc
      241 ttcaagatga tgttcttcga tggtttcggt ttgaatcggc tgaaacgtgc catacccgac
      301 gtgaagcgtt aattcaacaa tttcaacccc attgtctcga aggcgttgca acaattcggg
      361 agtaaagtgc agcccagcag ttggcgccgc aatggcaccg cgctcacggg catacacggt
      421 ttgataggtg tcgcgatcaa gccggacatc gcctgacgca taccggtgaa tatagggcgg
      481 tagcggcgtc tgaccaatgg catcaacgac ggtgtgaaat ggctcctctg actgaaactg
      541 gatgatgcga cggccatcgg gcgtgcgttc gacgacttcg ccacgcaaac gaccatcggc
      601 aaacgtgagt ttggtgccga ccgggatccg ccggcctggt ttgacgagcg aaagccagag
      661 attggggccc tgttcacgaa caaggaagac ctcgacccgt ccggtaaatc cctcccgatg
      721 gccaagcagt cgggcaggga acacacgggt attgttgaga accaggacat ctcctgggcg
      781 caaaaaattg gcaatttcaa aaaactgatg atcggaaaaa gtgccggtct ggcggtcaag
      841 ctttaacatt cgagaagcat cacgccgctg gcacggttct tgagcgattt gttcaggcgg
      901 aagatcgaaa tgcaattctg aaagtttcat ttgaagtcat tgcctggagg ttggcatctg
      961 gtgcttggtg cctggtacct ggtacttagg aaatcaatac ctttgaaagg ccaagaacga
     1021 agaaccagga accaagaacg aaggatttgg gttgatgaat gatgctgagg tgtaatggag
     1081 tcgtcatggt tggggaagaa tctcagaatt tcaaccaaaa tttgctgccc tggccaaatt
     1141 tatagatttt cagaataaga aaccacggaa tacacggaat acacagaaaa taaattcaga
     1201 ttttttcaat agttttcgag acttcccaaa atatagcctt tcagataaat atttcctggg
     1261 aacgccggca tcttgccggc accaagcggt tca
//
LOCUS       DAOQDO010002198         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130359_length_1293_cov_2.620355, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002198 DAOQDO010000000
VERSION     DAOQDO010002198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130359_length_1293_cov_2.620355"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKZ53_25980"
     CDS             <1..411
                     /locus_tag="PKZ53_25980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43958.1"
                     /translation="EQEFARERLKLTEMLTRRDTEFHNALAQQAAQNEAKNKQAQHEL
                     DLKSAEQDIENNLSDAFLHRELIHTLPEMLEHLPKPDELRSVSIGSGTEGAGQSLVSV
                     VGQILSLAEAFGFTRNRSMSHPIADQLAHPKVDE"
     gene            739..861
                     /locus_tag="PKZ53_25985"
     CDS             739..861
                     /locus_tag="PKZ53_25985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43959.1"
                     /translation="MPEPNEENLNPQTEENQEELQVKTQIKAGDISEDEPQDGI"
BASE COUNT          385 a          309 c          288 g          311 t
ORIGIN      
        1 gagcaggaat ttgcccgcga acgcctgaag ctcactgaaa tgttgacccg ccgggatacc
       61 gagtttcaca acgccctggc gcagcaagcc gcccaaaacg aagccaaaaa caaacaggcc
      121 cagcacgaac tggacctgaa atcggctgaa caggacatcg agaacaacct ctcggatgcc
      181 tttttgcacc gcgaattgat ccacacgctg cccgaaatgc tggagcacct gcccaagccg
      241 gacgaattgc gttccgtttc aattggttcc ggaacggaag gggccggaca atcgctggtg
      301 tcagttgtcg gccagatcct gagcctggcc gaagcctttg gcttcacgcg aaatcgttcg
      361 atgtctcacc ccatcgccga ccagcttgct catcccaaag tggatgaata gagtgtttaa
      421 aaggtgcaga acttaagcct tcgtttataa tgccagttaa gggttgaaaa aattgttcgt
      481 tgaaaacaaa gggcttatcg agatttcaaa ttttagacgt tcaatttgct gtctgtaagt
      541 tgttgatttt gaagtctcaa ttctaaccct taattggcat ttatattctc tcagtaccag
      601 aaatcttcaa tgaaatcaaa gattcggatt gcttttgtgt tcggagaatt tagaatagcc
      661 caggtgtcag aaaaaaatgt ttcccttagt atttcttgag tgaatgaact ctactttcta
      721 ttgtggaagg aaaaatgtat gcctgaacca aatgaagaaa acctgaaccc tcaaactgaa
      781 gagaatcaag aggaacttca ggtaaaaact caaatcaaag cgggtgatat atccgaagat
      841 gagccccaag atgggatata agggttgtga tgtttagcag tttgtggctg aaagtaacct
      901 gctgaatcaa atgtaccctc gtacttcaaa aaggttgtag cctcggaaaa actgaggcta
      961 caaccttttt ttgtactgca gtcagcgaga tgcatttgtt ctttgtaagt tgaactgcca
     1021 atccgaacgg tggcaagata gaggaaaaaa catggttgcc ccaaccagac gaaaaaagca
     1081 ccttccaccc aaactccacc aactctgagt atcgcctgct ctccatgaca cacccaaacg
     1141 attgtcgtga actcactcca tttcaactga cccaggaaat ggaatcgcta tttgctgaat
     1201 tgactacgat ccgggccgag atgatcaatc tggccagtcc ggctgaaacg attctggaca
     1261 aactcaaccc aacctaccat gaaagcgccc gca
//
LOCUS       DAOQDO010002199         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130376_length_1293_cov_2.456381, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002199 DAOQDO010000000
VERSION     DAOQDO010002199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130376_length_1293_cov_2.456381"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKZ53_25990"
     CDS             <1..472
                     /locus_tag="PKZ53_25990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43960.1"
                     /translation="YENGKTGTPYLVFPGVSDVGENDSGKAGKLLFFPSRQDCFQIEQ
                     MGDGSLVKTGEEIVFILSPKRLAELPPLPDDQPRAVDTSLFGKWVSSWSAPTWQFENQ
                     STVGNTLTLIEKSAELTNSAVLTTDAPLPQSVFHVKSIPGQPILFRLPLKIRKR"
     gene            480..>1293
                     /locus_tag="PKZ53_25995"
     CDS             480..>1293
                     /locus_tag="PKZ53_25995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ43961.1"
                     /translation="MLLKDQYQVIKKLGSGGMGEAFLALDLVSHQQVAVKLLHHGVDH
                     SETQHHWITQHFLNEAKALRLIDHPGVVKLIETGETEDGHQFLVMEFIEGASLRSQID
                     PYYGLLGGFERVAVLVRQLGEALSAAHEAGVYHRDLKPENILLTEVGSPNEQIKVIDF
                     GIATVKDRLDDQTKTTVLAGSLHYFAPEQLDGHPTAASDIFALGVIAYELVTGRTPFR
                     PDRPYPAAIFQLSELHQAGVRVNPKDLRPSLPVAAQEIILKALSYQPTARPAT"
BASE COUNT          340 a          298 c          313 g          342 t
ORIGIN      
        1 ctatgaaaat ggaaaaacag gaactcccta tctggttttt cctggagttt cagatgtggg
       61 tgaaaatgat tcaggaaaag ccggcaaatt gctgtttttt cccagtcgtc aggattgttt
      121 tcagattgag cagatggggg atggcagtct tgttaaaact ggggaagaga ttgtttttat
      181 cctttctccg aagcgtctgg cagaattacc gcccttacct gatgatcaac caagggccgt
      241 ggatacttcc ctctttggta aatgggtgag cagttggtca gcgccaacct ggcaatttga
      301 aaaccaatcc accgttggaa acacgttaac actgattgaa aaatcagccg aattgaccaa
      361 ttcggctgta ttgacaactg atgctccgtt acctcaatct gtttttcacg tcaaaagtat
      421 tccagggcaa cccatattat ttagacttcc gttaaaaatc cggaaacgct gattcaaata
      481 tgctactcaa agaccagtat caggtgatca aaaaattggg gagcggagga atgggagaag
      541 ctttcctggc gcttgatctg gtttctcatc agcaggttgc ggtcaaattg cttcatcacg
      601 gcgtagacca ttctgaaacc caacaccact ggatcactca acattttctg aatgaggcta
      661 aagcgcttcg gctcattgat catccaggtg tggtgaaatt gattgaaacc ggggaaacag
      721 aggatgggca ccaattcctg gttatggaat ttattgaggg agcttcgttg cgatcacaaa
      781 ttgatccgta ttatggtttg ttggggggat ttgagcgggt agcggtactt gttcgccaac
      841 ttggcgaggc tctttctgcg gctcacgaag caggggttta ccatcgtgat ttaaaacccg
      901 aaaatatctt attaacggaa gtcgggtcgc ccaatgaaca gataaaggtg attgattttg
      961 gaattgcaac cgtgaaggac aggctcgacg accaaaccaa aaccacagtc ctggcaggga
     1021 gtcttcacta ttttgcccca gagcaactcg atgggcatcc aaccgctgcc agtgatattt
     1081 ttgccctcgg cgttattgcc tatgaactgg taacgggccg cacccccttt cgtccggatc
     1141 gtccatatcc agccgcaatt tttcaacttt cagagctgca tcaagccggg gttcgggtca
     1201 atccaaaaga tttgcgtcca agcctgccag ttgccgccca ggaaattatt ctcaaggcat
     1261 tatcgtacca accaactgcc cgtccagcga ctg
//
LOCUS       DAOQDO010002200         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130384_length_1293_cov_2.399031, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002200 DAOQDO010000000
VERSION     DAOQDO010002200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130384_length_1293_cov_2.399031"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>1293)
                     /locus_tag="PKZ53_26000"
     CDS             complement(107..>1293)
                     /locus_tag="PKZ53_26000"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ43962.1"
                     /translation="LVQYWDLQRSRSFPNPRALRPDLPEAGHRIIEKALAFHPQDRFA
                     SVAQFEQELLQTLSGSPGYRVNSVPASPSHSFHLAETLVSTAPNLPKPVSEYPPAITA
                     ETLPGVGVSASSLPRHLSSALPGPQSGLSDLPGQTPSVFSPSRPLDATIAAPHPPLVQ
                     PVVQSADSVSPLQAVQTSRTRAPIIWVIPALVVVAALVGFIALLVINQVEDRDPVPAA
                     QPSKPVPPVPALEGKVSLKHKISQQTDSFRLETSEKNILCETGDELRLSIKVLKTGYV
                     YILNESPSLNAQTLPNFVILFPSKFDNQGSSRFETGHEIYLPPSTSARGLVLGNDKGT
                     EKLWLVWSLESVAELEAVKTLANPRDQGIVRKKEQIEAVTKALARQQGQTTVHCLKLE
                     HH"
BASE COUNT          277 a          303 c          397 g          316 t
ORIGIN      
        1 ggttgcgagt tgtgccctga acagatgagc ggtggtcttt tggcaaggca ttgctatttt
       61 gggcgaaatt caggtcattc ttcattcttc attcttcatt cttcattcaa tggtgctcga
      121 gtttgaggca atgaactgtg gtttgaccct gctggcgagc gagggctttg gtaacggctt
      181 cgatttgttc ttttttgcgg acaatgccct ggtctcttgg attggccagg gttttgaccg
      241 cttcgagttc agccacgctt tcaagtgacc agaccagcca gagtttttcc gtgcctttgt
      301 cattccccaa gaccagccct ctggcactgg tcgagggcgg aagataaatc tcgtgaccag
      361 tttcaaagcg ggatgacccc tggttgtcaa atttagaggg aaacaaaata acgaaattgg
      421 gcaaagtctg ggcattcaaa ctggggcttt cattcaaaat gtagacatac ccggttttga
      481 gaactttgat tgacaatcga agctcatccc cggtttcaca gaggatgttt ttctcagatg
      541 tttccagccg gaatgaatca gtttgttggg aaatcttgtg tttgagcgac accttgcctt
      601 cgagtgcggg taccggtgga acgggctttg atggttgagc tgccgggaca gggtcccggt
      661 cttcaacctg attgatcacc aggagagcga taaacccaac cagggctgca acgaccacca
      721 gggctgggat gacccagatg atgggggctc gggttcggct ggtttggacc gcctggagcg
      781 gcgaaacact gtcggctgat tggacgactg gttgaaccag aggtggatgc ggggcggcta
      841 tcgtcgcatc cagcggtctt gaaggtgaaa aaaccgatgg cgtttgaccc ggcaggtctg
      901 aaagccctga ctgtggcccg ggcagggcgg atgacaggtg gcgaggcagg ctgctggccg
      961 acaccccgac accgggtaag gtttcagccg tgatcgcagg tggatactct gaaaccggct
     1021 taggcaggtt aggtgctgtt gaaaccagcg tttcagccag atgaaacgaa tgtgatgggg
     1081 acgctggaac cgaattgacc cggtatccag gtgagccgga cagggtctgg agcaattctt
     1141 gctcaaactg ggcgactgag gcaaaccgat cctggggatg aaacgccagt gccttttcaa
     1201 tgatccggtg tcctgcttcc ggcaaatctg gccggagtgc ccgtgggttg ggaaagcttc
     1261 ttgagcgctg aaggtcccag tattgcacca gcg
//
LOCUS       DAOQDO010002201         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130391_length_1293_cov_2.334410, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002201 DAOQDO010000000
VERSION     DAOQDO010002201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130391_length_1293_cov_2.334410"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PKZ53_26005"
     CDS             complement(<1..1215)
                     /locus_tag="PKZ53_26005"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ43963.1"
                     /translation="MTFDHLTQEDGLSNNAIRTVFQDRKGFIWIGTEDGLNRYDGVQF
                     RVYRHQPNDPGSVPGSTILTIYEDRAGTLWFGTLSNGLGRYDSATDTFKTYPYQVAGG
                     LTKNFETPLCLYEDSSGNFWVGTVGGLLLFDRATGQFTGYPSLSQISSSITSMVEDQQ
                     GNLWAIVGFEIWIFDLTKRQFTERVYETATRSPRAILNSCLRLHPNGTVFALIAQEFL
                     VFDPNHRKVIDSYTGLIDGTLLEPEWAFMSLQFSDTSSAWVAVSGGGLIWVDLRAKSA
                     VMYRYDATVATSLIGSHPLALWLDQSGVLWIGDLASGISKYSRYKNKFRLYRHNPFDP
                     TSISNNYIRGMYEDHTGKLWVATQFGGLNVLDQQTGKFQKYPGHSDNGKSNVSSEMWA
                     VIEDHAHRLWVGG"
BASE COUNT          368 a          337 c          283 g          305 t
ORIGIN      
        1 tccaccaacc cacaaacgat gggcatgatc ttcaatcact gcccacatct cactggaaac
       61 attgcttttt ccattatctg aatgtcctgg atacttttga aatttcccgg tctgctgatc
      121 caggacattg aggccgccaa actgagttgc cacccacaat ttcccggtat gatcttcata
      181 catacctcga atgtaattgt tgctgattga cgtgggatca aagggattgt ggcgatataa
      241 ccggaattta ttcttatagc gtgagtattt gctgatcccg cttgccaaat ctccgatcca
      301 caaaacccca gactgatcca gccagagtgc cagcggatgg cttccaatca aggaagtggc
      361 aaccgtggca tcgtaccgat acataacagc cgattttgcc cgtaaatcaa cccatattaa
      421 accaccaccg gacacagcca cccaggctga agatgtgtct gaaaattgca gggacataaa
      481 tgcccattca ggttctaaga gggtgccatc aattagccca gtataggagt caataacttt
      541 tcgatggtta gggtcaaaaa ccaggaattc ctgagcgatc agggcaaata cggtgccgtt
      601 gggatgaagg cgtaaacaac tattgaggat tgctcgcgga gaacgagtag cagtttcata
      661 aacgcgctca gtgaattgcc gctttgtgag gtcaaagatc cagatttcga acccaacaat
      721 cgcccacaaa tttccttgct ggtcttcaac cattgaagta atggaggagc taatttggga
      781 gagtgaagga tacccggtga actgtccagt ggcccggtca aacagaagta atccccccac
      841 cgttcccacc caaaaatttc ccgaggaatc ttcatacagg cagagcgggg tttcaaaatt
      901 tttggtcaat ccgccagcga cctgataagg gtaggtcttg aacgtgtcag tcgccgaatc
      961 ataccgccct aacccatttg agagagtacc aaaccacagc gtaccggcac ggtcctcata
     1021 tatcgttaaa atcgtactgc ctggaactga acctggatca tttggctgat ggcggtacac
     1081 acgaaattga acgccgtcat agcgattcaa cccatcttca gtcccgatcc agataaatcc
     1141 tttccgatcc tgaaaaacag tccgaatggc attatttgac agaccgtctt cctgtgtcag
     1201 gtggtcaaat gtcacggatt ccgtttgagc gaatcccaca cttcccccac ccaataacca
     1261 aaggacgaca cacacgagta aacaaatcgg tac
//
LOCUS       DAOQDO010002202         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130519_length_1292_cov_4.177850, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002202 DAOQDO010000000
VERSION     DAOQDO010002202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130519_length_1292_cov_4.177850"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKZ53_26010"
     CDS             complement(<1..385)
                     /locus_tag="PKZ53_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43964.1"
                     /translation="MLYRIRWIGIVIVCCVVAVLPCVKPGSGWTQSQSNLTRGRLNAA
                     PTFPNLTGEKAIEHLKQEGAYDSLAAALAAARYSANWVANPRLPYLNGAYEADNPAQG
                     FTSYFTPEGVHLVAAGEGTPAWFLEM"
     gene            971..>1292
                     /locus_tag="PKZ53_26015"
     CDS             971..>1292
                     /locus_tag="PKZ53_26015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ43965.1"
                     /translation="MNFVEYINSFVPLSPATAEELFSKTQKDYLPKHYLLHKQGEICQ
                     KLYYLEQGLARWYYYNQDGKDITDSFGLEHSFVTAFDSFFQRKPSRYSIELLEDSIVF
                     SMTYA"
BASE COUNT          331 a          317 c          286 g          358 t
ORIGIN      
        1 tcatctccag aaaccacgct ggtgtacctt cccctgccgc taccagatgc accccttccg
       61 gcgtgaagta actcgtaaac ccctgggcgg gattgtcagc ttcataggca ccattcagat
      121 atggcagccg tggatttgcc acccagtttg cactgtagcg cgccgccgcc agcgccgccg
      181 ccagcgagtc gtatgccccc tcttgcttga gatgctcaat tgccttttcg ccggttaagt
      241 ttggaaatgt gggtgcggcg ttcaacctgc cgcgagtgag gttactctgg ctctgagtcc
      301 accctgatcc tggtttcaca cacggaagaa ctgcaaccac acaacaaaca ataacaattc
      361 cgatccatcg gatacgatac aacatacgtg tctccagcaa attacaggtt atccccattg
      421 gcaatgaagt cctcgcactt acaaattgtt cagcgattca accgaaaaaa tctggttgac
      481 gattgatggc tgacgacaaa ctggtcttcc agacacactg atgtgttaca cctggattat
      541 ataaggccga tttactggcc ttttcaggtc accaactcca tggtcttcaa ttttcaggta
      601 agcccttcag aatgctaaat aatgcggggt tcatatccgg gggtcactcg ttttcttatg
      661 gcatatctca aaagacattt caactggact ggagaaatca tggttcgggt ttgtgcggtg
      721 gatctatggt ccaattgaaa caggttgttg tcaggtttgt tgtttgagtt tgactgggtt
      781 gagcccaacc aaaaaccggc gggcgaaagg tcactggact ccagagcaca ttggaagttc
      841 cttgattttt cggtatgatg aatgatggta aagtgactcc cttccctttt taaactattg
      901 ttcataaaag aattatctca gtattttcgt tcttttcaga caatactcgg gcaaggagct
      961 tgaggagcat atgaatttcg tcgagtacat caattcgttc gttccattat cgccggcaac
     1021 ggcagaggaa ctcttcagca aaactcaaaa agactatctg cccaaacact atctgctcca
     1081 caaacaaggc gagatttgtc aaaaactcta ttacctcgaa cagggactgg cacgctggta
     1141 ctattacaac caggatggta aagatattac cgactccttt ggtctggaac attcatttgt
     1201 tactgccttt gatagtttct ttcaacgaaa gccaagccga tattcgattg agttactgga
     1261 agattccatt gttttttcta tgacgtatgc cg
//
LOCUS       DAOQDO010002203         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130680_length_1292_cov_1.303153, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002203 DAOQDO010000000
VERSION     DAOQDO010002203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130680_length_1292_cov_1.303153"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..788
                     /locus_tag="PKZ53_26020"
     CDS             294..788
                     /locus_tag="PKZ53_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNJ43966.1"
                     /translation="MAFDAFLKIEGIEGESTDGKHKGEIEILSYSWGISQPATVAAST
                     HGSLTAERANFGPFTIVKALDKASPKLSIACASGEHYPSAKLEICRAGGDKEPYMEYK
                     MTDVIVTSIHPGGSSKGAEAIPLEEVSFSYGKFELKYTKLDMKGKPAGNVAAGWDLKS
                     NKKV"
     gene            935..>1292
                     /gene="tssE"
                     /locus_tag="PKZ53_26025"
     CDS             935..>1292
                     /gene="tssE"
                     /locus_tag="PKZ53_26025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNJ43967.1"
                     /translation="MPPDIRTKPSVLDRLLNEEEPWTVASKGIRYFKEAVKRDIQWLL
                     NTRQTVDGIPPDLKELSSSVAAYGLPDFTTFNPKNLNDQAALRKLIETAIRTFEPRLE
                     HVVVRVLPLDETTRLLH"
BASE COUNT          366 a          318 c          311 g          297 t
ORIGIN      
        1 cacaatcaaa ctggatcgag tggttgtacc tcacacctcc ctgatccgaa tcaatcaaaa
       61 gccatgaacc aaagatggct taaccaaatt ttttgtgcct gtttgatcgc aatccctgac
      121 tcaatactca ggtaaagcca ttccaaactg gcattacgtt cgactcatca atcaggaaaa
      181 tgaaggatgg cggataacca ctgtctcaaa attcaatacc caggggttgc ccggcgatac
      241 ttcatttttc aaagtttgta accaatctac ttttattttt tggaggcact gctatggcgt
      301 ttgacgcttt tttgaaaatt gaaggaatcg aaggcgaaag cactgatgga aaacacaaag
      361 gtgaaatcga aatcctgtct tacagttggg gaatttccca gccagccacg gttgcagcca
      421 gtactcatgg ctcactgacg gcggaacggg ccaatttcgg cccctttacg attgtgaaag
      481 ccctggataa agcatcaccg aaattatcta ttgcctgtgc cagtggggag cattacccaa
      541 gcgccaaatt ggaaatttgt cgcgccggcg gcgacaaaga accctacatg gaatacaaaa
      601 tgaccgatgt catcgtcacc agtatccacc caggaggctc aagcaaaggg gctgaagcca
      661 ttccactcga agaagtttcg ttcagttatg gaaaatttga actcaaatac accaagctcg
      721 atatgaaggg caaaccggct ggcaatgtcg cggctggttg ggatttgaaa tcgaataaga
      781 aagtgtagtc aacctgtaag gttcagggtt tcggggtttt cgctatggcg tggtcgctca
      841 atgaacgcag gtcgtctatg gcctgtgaca aaagagaaca ggccaacgat tgacaaaccc
      901 ggaacccgga acccggaacc cagagaccga aagtatgcca cctgacattc gaaccaaacc
      961 atctgtattg gaccggttgc tcaatgaaga agaaccctgg acggttgcgt ccaaagggat
     1021 tcggtatttc aaagaagccg tcaaacgcga tatccagtgg ctgttaaaca cccgtcagac
     1081 cgtggatggg attccacctg acttgaagga gttatcaagt tcggtggcgg cgtatggcct
     1141 gccggatttc accacgttta atccaaaaaa cctgaacgat caggctgccc tccgcaaatt
     1201 gatcgaaacc gcgattcgca cgtttgaacc ccgacttgaa catgtggtgg ttcgggtcct
     1261 gccgctcgat gaaacgaccc ggctcctgca tt
//
LOCUS       DAOQDO010002204         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130755_length_1291_cov_2.525081, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002204 DAOQDO010000000
VERSION     DAOQDO010002204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130755_length_1291_cov_2.525081"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKZ53_26030"
     CDS             <1..735
                     /locus_tag="PKZ53_26030"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43968.1"
                     /translation="RPKSADIWGDNISQLTGAQYLLKFLLKTINSLNARFLPGKPDEV
                     FFWEDKVTQIPIALEVGFQVPETLVTTDFRQVVSFFNRHRAIISKAATAFPSGTSAST
                     TKAVSADDLIYLQRVRNYPVIFQERVEKQADIRVAVIGDQLFASQIRTQHTSRGKVDW
                     RAAFVREESVYELPTEIKEKCLALTHNMKLSYGAIDLVLTPEGKHVFIEINPGGQWMW
                     MEMDLGLPLSQAVAEYLMAGTGEASQ"
     gene            732..>1291
                     /locus_tag="PKZ53_26035"
     CDS             732..>1291
                     /locus_tag="PKZ53_26035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43969.1"
                     /translation="MKNDPTLSWLASLAEVAKKIAAAPDGLLADTLSELSILKQQFPE
                     NNVRVLFTKLPGRFDHEYDLSVEDGDGTFVLGFRPNSNLPWTSVHSRHDAYSKEESKD
                     VVLFRIDEIEYDGRTVLRMLEYSTRTEGISIEYFETHLTLLTCLLDCPETLETLLSKD
                     HITRIVQSFAGELPEQEKTDCFEQLR"
BASE COUNT          339 a          329 c          304 g          319 t
ORIGIN      
        1 cgccccaaat cggcggatat ttggggagat aacataagcc agttgaccgg tgctcaatac
       61 ctgctcaagt ttcttctcaa gacgatcaac tcgctcaatg cccggttcct ccctggcaaa
      121 ccggatgaag tgttcttttg ggaagataaa gtgacgcaaa tcccaatcgc gcttgaagtt
      181 ggctttcagg ttcctgaaac gctggtgacc accgatttcc gccaggtcgt cagttttttc
      241 aatcgtcaca gggccatcat ttccaaggcc gctaccgctt ttccaagcgg aacctccgcc
      301 tcaacaacca aagctgtttc ggcagacgat ttgatttatc ttcaacgggt tcgaaattat
      361 ccagtcattt ttcaggaacg ggtggaaaaa caagctgaca ttcgcgtggc ggtcatcgga
      421 gaccagcttt ttgccagtca gattcgaacc cagcatacct cgcgtggaaa agttgactgg
      481 cgagccgcgt ttgtccgaga ggaatctgtg tatgaactcc cgactgaaat caaagaaaag
      541 tgtctggcac tgacccataa catgaaactg tcctatgggg ccattgatct ggttttaacc
      601 ccagagggca agcacgtctt cattgaaatc aaccctggcg gacaatggat gtggatggaa
      661 atggatcttg gcctgccctt gtcccaggct gtggccgaat atctgatggc tggcaccgga
      721 gaagcttccc aatgaaaaac gaccccactt tgagctggct ggcctccttg gccgaagtgg
      781 caaagaagat tgccgctgcc ccagatggct tgcttgcgga tacactgagc gagctatcaa
      841 ttttgaaaca gcaatttcca gaaaataacg ttcgggttct gtttaccaaa ctccctggaa
      901 ggttcgacca cgagtacgac ctgtcagttg aagatggaga tggaaccttt gtcctgggct
      961 tcaggccaaa tagtaatctt ccctggacct cagttcattc gcgacacgat gcctactcca
     1021 aagaggaatc aaaagatgtc gtcctgtttc ggatagatga aattgaatat gacggaagaa
     1081 ccgtgctgag aatgctggag tattccaccc gaaccgaagg aatctcaatc gaatatttcg
     1141 aaacccatct cactctcttg acctgtcttc ttgattgccc tgaaacattg gaaaccctgc
     1201 tttccaaaga ccacatcaca cgcattgttc aatcatttgc aggtgaactc cctgaacagg
     1261 aaaagaccga ttgttttgaa cagttacgtc a
//
LOCUS       DAOQDO010002205         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_130936_length_1290_cov_3.600810, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002205 DAOQDO010000000
VERSION     DAOQDO010002205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130936_length_1290_cov_3.600810"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..640
                     /gene="scpB"
                     /locus_tag="PKZ53_26040"
     CDS             104..640
                     /gene="scpB"
                     /locus_tag="PKZ53_26040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNJ43970.1"
                     /translation="MHFDELKSVIEALIFVSDEPITLKQLVELIDEASPADIEVALQH
                     LESDYRERRSGLELREIAGGWRLSSRPEFHEYLRRYAKSKPSSRLSLAALETLAVIAY
                     KQPVTIPEILEIRGVSSSSAIKTLLDRKLIIPKGRKECVGRPMMYGTSKDFLLQFGLK
                     DLTGLPNIEDLEDLSATT"
     gene            731..>1290
                     /locus_tag="PKZ53_26045"
     CDS             731..>1290
                     /locus_tag="PKZ53_26045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ43971.1"
                     /translation="MLERLQKIIANAGLASRRKAEELIQEGLVTVNGQVVTELGTKAD
                     PAQDHIKVNGKLINPLLENSAKVYLLLNKPKGYLSSLSDPQNRPLVTEFIPAGMPRVY
                     PVGRLDFNTEGLLILTNDGDLTNVITKAGSHCPKVYHVKVKGSPTQEQLGRILRGMMI
                     EGELYKIAKLGKLEITDAANTWFELTL"
BASE COUNT          338 a          345 c          300 g          307 t
ORIGIN      
        1 agtaccaagt accaagtacc aaacaccagt caccaagtac caggcaccag gtacctgatt
       61 gcgaaattct gattgtcggc aaactcccac ttttctacca actatgcact ttgacgaatt
      121 aaaatctgtg attgaggcgt tgatttttgt ttcagacgag ccaattaccc tcaaacaact
      181 cgttgaactg attgacgaag ccagcccggc agatatcgaa gtggctcttc aacacctgga
      241 atcggactat cgcgaacgtc gctctgggct tgaacttcgc gaaattgctg gtggctggcg
      301 gttgtcgagt cgtccggagt ttcatgaata tttgcggcgc tatgccaaat ccaagccttc
      361 atcgcggctt tcgctggcgg cactggaaac cctggcggtc attgcctaca aacaacccgt
      421 cacgattccc gaaatcctgg aaattcgtgg cgtctcatca tcttcagcca tcaagacgct
      481 gcttgatcgc aagttgatta ttcccaaagg ccgcaaggaa tgtgtaggcc ggccaatgat
      541 gtatggcacc tcaaaagatt ttctgcttca gtttggactc aaggatttga cgggactgcc
      601 caacattgag gaccttgaag atttgtcggc cacgacctaa atcctgggcg cagttcaacg
      661 cagcgacgcc ccttacttct gggcgcagtt caacgcagcg acgcccctta cttctgattc
      721 atttgtattt atgctcgaac gactccaaaa aattattgcc aatgctggcc ttgcctcacg
      781 gcgcaaagct gaagaactca tccaggaagg actggttacc gtcaatggtc aggtggttac
      841 tgaacttggc accaaagccg atccggccca ggaccatatt aaagtcaacg gaaaactgat
      901 caacccgctg cttgaaaact ctgccaaagt gtacctgttg ctcaataaac ccaaagggta
      961 cctttccagt ctctcagacc cgcaaaatcg cccactggtg acagaattta ttccggctgg
     1021 aatgcctcgc gtgtatccgg ttggacggct tgatttcaat accgaagggc tactgatcct
     1081 gaccaacgat ggtgacctga ccaacgtgat taccaaagct ggcagtcatt gccccaaggt
     1141 ttatcacgtc aaggtcaaag gaagcccaac tcaggaacaa cttgggcgaa ttttgcgcgg
     1201 catgatgatc gaaggtgagc tgtataaaat tgccaaactg ggcaagctgg aaattaccga
     1261 cgccgccaac acctggtttg aattaaccct
//
LOCUS       DAOQDO010002206         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_131374_length_1288_cov_2.240065, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002206 DAOQDO010000000
VERSION     DAOQDO010002206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131374_length_1288_cov_2.240065"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1288
                     /locus_tag="PKZ53_26050"
     CDS             <1..>1288
                     /locus_tag="PKZ53_26050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43972.1"
                     /translation="TLEFISHIDFLLSEVNTWPQRNLTNQFLQEKIGTYYRGYRQQIV
                     DRLLQKAPSKAEAIPQTSPGVDPTPTPVAPPVAHQGSAIPGSPGRAEQFAAKLSEPPP
                     FKASPEPPKATFSVPPRPVPQPEPVPEISLLERLSDPQMLRWMVYSGAMMFVIGTIIY
                     LRDVISLQLQRPVVQAGLLALLTIATFGAGVWLLRRTQEELAGRGLLLLGSLLVPANP
                     WFLVHSGLIARGNRAWMVGLVCAVLYATTAMVLRETLMLYLAIPALLASVYGFLGAVV
                     HAPPDVYAAVTVLFAGGLLVLEQKLPELESDQEFAREVQKPFFLSGHLILAAGLFVYT
                     GLFRWLPFEVLAALQSVAGNLVGTISHPALIGVTLVGAALYGMTAYTRQNSAWSYLST
                     GLLLTVAVNELIWFHAPFVITLFTFALCALGLLLASR"
BASE COUNT          249 a          360 c          342 g          337 t
ORIGIN      
        1 cgacactgga gttcatttcg catattgact tcctgttatc tgaggtcaac acctggccgc
       61 aacgcaacct gaccaaccag ttcctgcagg aaaaaattgg cacctattat cgagggtatc
      121 gccagcagat tgtggaccga ttgctccaga aagctccatc gaaggcggaa gcaattcctc
      181 aaacctctcc gggcgttgat ccaacaccaa cgccagtggc accgccagtt gcccatcagg
      241 gttcggccat ccctgggtca ccaggtcgag ccgaacaatt tgcagcaaag ttaagtgaac
      301 ctcctccgtt taaagcgtct cccgagccgc caaaggcaac attttcagtt cctccccgcc
      361 ctgttcctca gccagaacca gttcccgaaa tcagtttgct ggaacggctg tctgacccgc
      421 aaatgcttcg ctggatggtc tatagcggcg ccatgatgtt tgtcatcggg acgattattt
      481 acctgcgcga cgtcatcagc ctgcaattgc aacgcccggt ggtccaggcc ggattgctag
      541 cgctcctgac aatagccact tttggcgctg gagtgtggtt gctgcgccga acccaggaag
      601 aactcgccgg acgtgggctg ctgttacttg gttcgctttt ggtacccgcc aatccgtggt
      661 ttctggtgca ttctgggttg attgcgcgtg ggaatcgagc ctggatggtt ggtctggtct
      721 gtgcggtgct gtatgcgaca accgcgatgg tgctccgcga aacattgatg ctgtatctgg
      781 ccattccagc tttgctggcc agtgtgtatg gatttttggg ggcagtggtt cacgctccac
      841 ctgatgtgta tgctgctgtc acggttctat ttgctggcgg gctactcgta ctggaacaaa
      901 aacttcccga acttgaatca gaccaggaat ttgcccgcga agtccaaaaa ccgttctttc
      961 tctcgggaca cctgatcctg gcggctggat tgtttgtcta taccggattg tttcgctggc
     1021 ttcccttcga ggtactggcg gcacttcaga gcgtagctgg aaatctggtc gggaccattt
     1081 cacatccggc attgattggg gtcacactgg ttggggcggc actctatgga atgactgcct
     1141 atacccggca aaattctgcc tggagctatc tttcgactgg tttactactc acggtcgccg
     1201 tcaacgaact catctggttt cacgctccgt ttgtcattac gctgttcact tttgcgttat
     1261 gcgccctggg actcctgctc gcgtcacg
//
LOCUS       DAOQDO010002207         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_131375_length_1288_cov_2.230333, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002207 DAOQDO010000000
VERSION     DAOQDO010002207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131375_length_1288_cov_2.230333"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1206
                     /gene="hypE"
                     /locus_tag="PKZ53_26055"
     CDS             133..1206
                     /gene="hypE"
                     /locus_tag="PKZ53_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNJ43973.1"
                     /translation="MSKPLNFNEWTCPLPLRDHPNILMSHGGGGKLSAELVENLFLPA
                     FGESALRSLHDSAALEMSHLLGNSTRLAFSTDSFVVKPLFFPGGNIGDLAVNGTVNDI
                     AMSGAQALFLSTGFILEEGLAMQDLGKIASSMGAAALQAGVKLATGDTKVVNRGHGDG
                     VYINTSGFGVIPAGVHIGPDQARPGDVVIVSGEIGVHGIAILSVREGLEFETVIETDC
                     APLNGLVAAMLEVSPSIHVLRDPTRGGLASSLNEIAKASQVGISFVERLLPVPPQVKS
                     ACEILGLDPVYVANEGKLLAIVPRDVAEPMLEAMKRHPMGHQAAIIGEVTDRHPGFVV
                     AKTGIGGTRVVDMQLGEQLPRIC"
BASE COUNT          307 a          342 c          342 g          297 t
ORIGIN      
        1 gcaattgaag ggatgaggga tgaaggatga gggatgaaat aaaaccatgt cttcagccca
       61 tcttcttcag cccgatttct tcaacccatt ttcttcagcc ccgagcttgc gagtcttcaa
      121 ccccaaaatt ctatgtccaa accactcaac ttcaatgaat ggacctgccc actcccgctg
      181 cgtgaccacc cgaacattct gatgagtcac ggcggaggtg gaaaattgtc ggctgaactg
      241 gtcgaaaacc tgtttctgcc agcttttggg gaaagcgcgc tccggtcgct gcacgattca
      301 gccgcactgg agatgagcca cctgctggga aattccaccc gccttgcctt ttcaactgat
      361 tcatttgtcg tcaagccgtt gttttttccc ggtggcaaca ttggcgatct ggccgttaac
      421 ggaacggtca acgacatcgc tatgagtggc gcccaggcgt tgtttttgag caccgggttt
      481 attctcgaag aaggcttggc aatgcaggac ctgggaaaga tagcgagttc gatgggggct
      541 gcggcacttc aagctggagt caaactggcc actggcgata ccaaagtggt caatcgtggc
      601 cacggagacg gcgtctatat caacaccagc gggtttggtg tgattcctgc aggtgttcat
      661 atcggcccag atcaggctcg ccccggagat gtggtgattg tcagcggaga aatcggcgtc
      721 cacgggattg cgattttgag cgttcgcgaa ggtctggaat ttgaaaccgt gattgaaacc
      781 gactgtgctc cgcttaatgg actggtcgcg gcgatgctcg aagtctcacc atctatccac
      841 gtgctgcgtg acccaacccg aggtggtctg gcatcgtcac ttaatgaaat tgccaaagcg
      901 tcacaggtcg ggatttcatt tgttgaacgc ctgctgccag tgccgccgca agtcaaatct
      961 gcctgtgaaa tcctggggtt ggatcccgtg tatgtcgcca acgaaggcaa actcctcgca
     1021 attgtccccc gcgatgtggc cgaaccaatg ctggaagcca tgaaacggca tccgatgggt
     1081 caccaagccg ccatcatcgg cgaagtcact gaccggcatc cgggttttgt cgtcgccaaa
     1141 accggtatcg gaggaacgcg ggtcgtggat atgcagcttg gcgaacagtt acccagaatt
     1201 tgttaagtcc cttaccgaaa atttccagac atgttgaacc acgaaataca cgaaatacac
     1261 gaaaaaaacc aaacacttcc aacattca
//
LOCUS       DAOQDO010002208         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_131729_length_1286_cov_2.632819, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002208 DAOQDO010000000
VERSION     DAOQDO010002208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131729_length_1286_cov_2.632819"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKZ53_26060"
     CDS             <1..644
                     /locus_tag="PKZ53_26060"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ43974.1"
                     /translation="WDYRNLVELLITSGADPNVRDNDGDTPLHLVSYNGHYAVAQYLV
                     SRGANVNIKNKDGNTPLHWVARGKRKNIADLLISQGADVNARNNEGRIPLHEAARFGR
                     SGSLVSVFLHRGARVNEPDNYGNTPLFFAAGCGFRDVVELLIIHGADVMAHDEDEETP
                     LHWAAMSGQVEIAEYLFVNGAELTVKNQEGKTPIDVAPNDRIREVLMGLLRKS"
     gene            complement(753..>1286)
                     /locus_tag="PKZ53_26065"
     CDS             complement(753..>1286)
                     /locus_tag="PKZ53_26065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43975.1"
                     /translation="TDVQACIPSVLVSPVELNSEEKHLIQMTFTHRGIQPLVGGLILA
                     SRQLASGSSPQSRWGLPPKILRSYRVAITGSQTSETDLAALYIRDVCVVFDAQDIWLA
                     LACYGQPLWRVATVRALLETKDPDRQLRTDLWKCYQAGIINQEEWHQLCWILAASTIA
                     EHPAAYGLTGKPLSAQL"
BASE COUNT          308 a          310 c          365 g          303 t
ORIGIN      
        1 attgggatta ccgcaatctg gttgaattat tgattaccag cggagctgat cccaatgtgc
       61 gggataatga cggtgataca ccgctgcatc tggtttctta taatgggcat tatgcagtgg
      121 ctcaatatct ggtctcacgc ggtgccaacg tcaatattaa aaataaggac ggaaatacgc
      181 cgttacactg ggtggcacgc ggaaaacgaa aaaatattgc tgacttgttg atttcgcagg
      241 gggctgacgt caatgcccgc aacaacgaag gccgcattcc gctgcacgag gccgccagat
      301 ttggacggag cgggtcactg gttagtgttt ttctccatcg aggggccagg gtgaacgagc
      361 ctgacaatta tgggaacacg ccactctttt ttgccgccgg gtgtggattt cgcgatgtgg
      421 ttgaactcct gattattcat ggtgccgatg tgatggccca tgacgaggat gaagaaacac
      481 ccctgcattg ggccgccatg agcgggcagg tggagattgc cgaatatctg tttgtgaatg
      541 gcgcagagtt gacagtgaag aatcaggaag gaaaaacccc aattgatgtc gcgccaaatg
      601 accgcattcg ggaggttctc atggggttac tgcgcaagtc ctgactgaac gaactccaca
      661 caaagagatt tgggctgaag aacctcgggg aagaggttgg gctcagggcc tggatctcaa
      721 ttcccaggcg cttcagccca cagttttcag ttctacaatt gcgctgataa cggtttccca
      781 gtcaggccat aggctgctgg atgttcggca atggtgctgg ccgccagaat ccagcagagt
      841 tggtgccatt cttcctggtt gatgatccca gcctgataac acttccaaag gtcagttcga
      901 agctgccggt ctggatcttt ggtttcgagc agtgcccgta ctgtggccac tcgccagagt
      961 ggctgaccgt aacaggccag cgccagccag atatcttgag cgtcaaaaac cacacataca
     1021 tctcgaatat agagtgccgc caggtctgtt tctgaggtct gtgatcccgt tatggcaaca
     1081 cggtaggatc gcagaatttt tggcggaagc ccccagcggg attgaggtga agaccctgaa
     1141 gccagttgcc gggaagccag aatcaatccc cccaccagcg gttgaatccc tcgatgcgta
     1201 aatgtcatct ggatcaggtg tttttcttcc gaattgagtt ccacagggga taccaggact
     1261 gaaggaatac aggcctgtac gtctgt
//
LOCUS       DAOQDO010002209         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_131732_length_1286_cov_2.623883, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002209 DAOQDO010000000
VERSION     DAOQDO010002209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131732_length_1286_cov_2.623883"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKZ53_26070"
     CDS             complement(<1..537)
                     /locus_tag="PKZ53_26070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ43976.1"
                     /translation="MTSFVAAFLARVSPLVFFMASAMAPDRWQKFSESGNISYLYTLD
                     TFDLTIISLYFGLLAILSIYGIYRIRMTYLYWRYRDHSPKPARLYSEDELPKVTIQLP
                     LFNEMYVVERLLESVVQIDYPQGKLEIQVLDDSTDETQHIAAAAVKRHAEAGHNIVYI
                     HRDNREGFKAGALEAGLKV"
     gene            complement(638..>1286)
                     /locus_tag="PKZ53_26075"
     CDS             complement(638..>1286)
                     /locus_tag="PKZ53_26075"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ43977.1"
                     /translation="GMKLFLDGPVGKLEAIFETAGTSAGLKALVCHPHPVHGGTMHNR
                     VVVRIARALLAVGVDVLRINFRGTGDSEGSYDAGVGELQDALAAIHFLEQQRPGWPLI
                     VGGFSFGSWVSFRAGLQVPQTCGLLAAGLPVRHYDFDFVSRSPLPKWVIQGEFDEFGN
                     SRQVAAVVDTFANPKGFSFVPEADHYFSDHIDVFSARLTEAATWLRTQVLPEVTR"
BASE COUNT          345 a          346 c          333 g          262 t
ORIGIN      
        1 caccttgagg cccgcttcaa gggcgccagc tttaaagcct tcacggttgt cacggtgaat
       61 gtagacgata ttgtgaccag cttcagcgtg gcgtttgacg gccgccgccg caatgtgctg
      121 ggtttcatcg gttgaatcgt cgaggacctg aatttcaagt ttcccctgtg gatagtcaat
      181 ttgaacgacg gattcaagca accgctccac gacatacatt tcattaaaga gtggaagctg
      241 aatcgtcact ttcggaagtt catcttcgct gtagagtcgg gcaggtttgg gcgagtgatc
      301 ccggtaccgc cagtacagat aggtcatccg aatgcgataa atcccataaa tcgaaagaat
      361 tgccagcagg ccgaagtaca gcgagatgat tgtcaggtca aacgtatcga gcgtgtagag
      421 atagctgatg ttgcccgact cagaaaattt ctgccatcgg tccggcgcca ttgccgacgc
      481 catgaaaaaa accaggggtg ataccctggc caaaaatgca gcaacaaaac tggtcatgcc
      541 aataccctca aaaaatttca caccacaaag cgggaaagaa aaagtgcaga tgggagagag
      601 cgattccggg tcgctcagga aaacccgtaa ctatacgtta tcgtgtgact tctggcaaga
      661 cctgagttcg aagccaggta gctgcctcgg tcagtcgagc ggaaaacaca tcaatatggt
      721 cagaaaaata gtggtcggcc tcaggcacaa agctaaaccc ttttgggttg gcaaatgtgt
      781 ctacaacggc ggctacctgc cgggaattcc caaactcatc aaattcgccc tgaatgaccc
      841 actttggaag aggggagcgg ctgacaaaat caaaatcgta gtggcgaacg ggaagtccag
      901 ccgccagcag cccacaagtt tgcggcacct gcagcccggc ccggaaactg acccaggaac
      961 caaaggaaaa gcccccgaca atcagtggcc agcctggtcg ttgctgttcg agaaaatgaa
     1021 tcgcggcaag tgcatcctgc agttcaccaa cgccagcatc atagctgccc tcactgtcac
     1081 cggttccccg aaaattgatc cgtagcacat caaccccgac cgccaggagt gctctggcaa
     1141 ttcgcacgac cacgcggttg tgcatcgttc cgccgtgtac cggatgtgga tggcacacca
     1201 gggctttgag tccggctgaa gttccagccg tctcaaaaat ggcttccagt ttgccaactg
     1261 gcccatcaag aaagagtttc atcccc
//
LOCUS       DAOQDO010002210         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_131963_length_1285_cov_2.405691, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002210 DAOQDO010000000
VERSION     DAOQDO010002210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131963_length_1285_cov_2.405691"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKZ53_26080"
     CDS             <1..210
                     /locus_tag="PKZ53_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43978.1"
                     /translation="ALVDGLNHGHPIKEILFIQADVTRQKRWKHAEEAAKSAEAQTEF
                     PLMVSVIGAVLILVVPLVLRVFQTL"
     gene            complement(285..>1285)
                     /locus_tag="PKZ53_26085"
     CDS             complement(285..>1285)
                     /locus_tag="PKZ53_26085"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43979.1"
                     /translation="QIQAVSQRLYQRVVKPVRELVGNTRHLLISPDGGLNLVPFAALQ
                     DETGKYLVEQYSLTFLTSGRDLLRLKVKIPSQDPAVVVADPTYGDGTGPVLLGKGIGR
                     LRQLDGTKAEGSFLKRLFPKAQVWMENQATKDRLKAVSKPALVHIATHGFFLEDAPKE
                     PLNGTDFQRGITLGDTPPPVNVEQLREANPLLRSCLFFAGANGTANSTGESGTMTALE
                     ATGLDLWGTKLVVLSACDTGLGEVKNGDGVYGLRRALVLAGSEAQMMSLWPVSDEGTK
                     ELMQQYYTRLKAGEGRSEALRQVQLQMLKSKRWNHPFYWASFIQSGEWANLNGKRK"
BASE COUNT          273 a          356 c          329 g          327 t
ORIGIN      
        1 gccctggttg acggcttaaa tcacggccat ccaatcaaag aaatattgtt tatccaggcc
       61 gacgttaccc gtcaaaaaag atggaagcac gctgaagaag ccgcgaaatc agccgaagcc
      121 caaactgaat tcccgttgat ggtatcggtc atcggcgccg tcctgatcct ggtggttcca
      181 ctggtacttc gggtctttca aaccctttaa aaaaatttgg agtgctgtga cttgtcgcag
      241 ctttggtact cagcgatcta ttgaaaaggt gtctttcccc gtgttcactt tcgtttcccg
      301 ttcaggttgg cccattcgcc agattgaatg aagctcgccc aataaaaggg atgattccac
      361 cttttgcttt tcaacatctg gagttgtacc tggcgcaggg cttcgctacg gccttcaccg
      421 gctttgagtc gggtgtaata ctgctgcatc agttccttgg tgccttcgtc tgaaactggc
      481 cagaggctca tcatctgggc ttcgctgcca gccagcacca gtgcccggcg gagtccgtac
      541 acgccgtctc cgtttttgac ttctcccaat ccggtgtcac aggccgagag caccaccagc
      601 ttcgttcccc agagatcaag cccggtggct tcgagagcgg tcatcgttcc tgattccccg
      661 gttgaattgg ctgtgccatt ggcaccagcg aaaaacaaac atgacctcag cagcgggttt
      721 gcctcccgga gttgctcaac attcacgggt ggtggagtgt cacccagcgt tattccgcgt
      781 tggaaatcag ttccgttcag tggctctttg ggcgcatcct ccaggaaaaa gccatgggtt
      841 gcaatatgaa ccagtgccgg tttggagaca gccttgagtc tgtctttggt cgcctgattt
      901 tccatccaga cctgggcttt gggaaagagc cgtttgagga aggaaccttc agctttggta
      961 ccatcaagtt gtcggagtct tccgatacct ttcccaagca acactggtcc ggtgccgtct
     1021 ccataggttg gatcagccac caccaccgcc ggatcttgac ttggaatttt tactttcaac
     1081 cggagcaaat cccggccact ggtcagaaag gtcagcgaat actgttcaac caggtacttt
     1141 ccggtttcat cctggagtgc cgcaaatgga accagattca atccgccgtc cggcgaaatc
     1201 aagaggtgtc gggtgtttcc gaccagttcg cgaacgggtt tcacaacccg ctggtacaaa
     1261 cgttgggaca ccgcctggat ttgct
//
LOCUS       DAOQDO010002211         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132315_length_1283_cov_3.618078, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002211 DAOQDO010000000
VERSION     DAOQDO010002211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132315_length_1283_cov_3.618078"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..728)
                     /locus_tag="PKZ53_26090"
     CDS             complement(42..728)
                     /locus_tag="PKZ53_26090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNJ43980.1"
                     /translation="MPQDQFTVSVIVPVYNAAPYLAEALTSLQSQSLKPFEIIVIDDG
                     STDDSATIAQSFAGVRLIQQPNSGPAAARNTGINVAQGTVIGFLDADDLWPVDKLETQ
                     LGVLDANPEAEIVVGQIQLWRLMDATSRHFEPFSEPWTSVNVGSVLIRRSVFDRVGLF
                     DSNLRQSEDVDWFVRTREQGIKLVTHPEITLFYRIHTANLTSDKAARDQYLLTAVKRS
                     LDRRRKTNEE"
     gene            867..>1283
                     /locus_tag="PKZ53_26095"
     CDS             867..>1283
                     /locus_tag="PKZ53_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ43981.1"
                     /translation="MPALSKLTVPVVLIIFHRPDTTAQVFERIREAAPAHLFVIADGP
                     RLTHPDDAQACAATRAIIQKVDWDCTVQTRFSDVNLGCHLNITTGLDWVFEHVPEAII
                     VEDDCLPHPTFFRFCAELLERYRDEPQIMTVCGNNFQ"
BASE COUNT          319 a          337 c          293 g          334 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattcttc atttgtcttc
       61 cgacgacggt caagtgaacg cttgaccgca gtcagtaaat actgatcacg agctgcttta
      121 tcactcgtca gatttgcagt atggattcga taaaaaagag ttatttctgg gtgggtgacg
      181 agctttattc cttgctcgcg ggtccgcaca aaccagtcaa catcctcact ctgtcgtaaa
      241 ttggaatcaa acagcccaac ccgatcaaaa acagaccttc gaatcaggac actgccgaca
      301 ttgaccgatg tccagggctc ggaaaatggt tcgaagtggc gtgaggtggc atccatcagc
      361 cgccagagtt gaatctgccc aaccacaatt tcagcttcgg ggttggcatc gagcacgcca
      421 agctgagttt cgagtttatc cacaggccag agatcatcgg catccagaaa accaatcacc
      481 gttccctgag ccacattgat tccagtattg cgggcagcgg ctgggccgct gtttggttgc
      541 tgaatcaacc ggactccagc aaatgactgc gcaatcgtgg ctgaatcatc ggtcgaacca
      601 tcgtctataa caatgatttc aaagggtttc aggctctgcg attgaaggct ggtcagcgct
      661 tcggccagat acggtgcagc gttatacact ggcacaatca cacttaccgt gaattgatct
      721 tgcggcatat ccacaaataa agaggagtct ttcagttggg agtttcaagt tacgaagttg
      781 aaaaatttag acgagatagc gcatgctgga aagtgaaaag tccatcaatt tgttccaaat
      841 ctccatttca tctctcgaat gtccaaatgc cagccctctc aaaactgaca gttccggtcg
      901 ttctgatcat ctttcatcgt cctgatacca ccgcccaggt ttttgagcga atccgtgaag
      961 cagcaccagc acacctcttt gtgattgctg acggaccgcg cttaacccat ccggatgacg
     1021 ctcaagcctg tgctgccacc cgggccatca ttcaaaaagt tgactgggac tgcaccgtcc
     1081 agactcgttt ttccgatgtc aatctggggt gccacctcaa tatcacaact ggtctggatt
     1141 gggtgtttga acacgtgccg gaagcaatta ttgtggaaga cgactgcctg ccgcatccga
     1201 cgttctttcg gttttgcgcc gaactgcttg aacgctatcg tgatgaacca cagataatga
     1261 ccgtgtgcgg caacaacttt cag
//
LOCUS       DAOQDO010002212         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132374_length_1283_cov_2.379479, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002212 DAOQDO010000000
VERSION     DAOQDO010002212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132374_length_1283_cov_2.379479"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKZ53_26100"
     CDS             complement(<1..585)
                     /locus_tag="PKZ53_26100"
                     /inference="COORDINATES: protein motif:HMM:NF013729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNJ43982.1"
                     /translation="MHIAILHERQSQPDDYVAQILESALTAQGCQVFIDRSQTDGIEW
                     VREIKNQVRTADVVIVLLSGTSVSGETLAYEIRIAFETAQQQGKPKVLPIRLNFLDPL
                     PRPLTGILPEPIIFSWMSPQDNLSLTQAVLSILNPAGPVVIPSELPPQPLEPVGGAVP
                     LNSKYYILRRMDEEFGTAISRGDSIVLVKGARQMG"
     gene            complement(718..>1283)
                     /locus_tag="PKZ53_26105"
     CDS             complement(718..>1283)
                     /locus_tag="PKZ53_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HNJ43983.1"
                     /translation="KFNILRHLTKIGCRVTVVPATTTFEEVQKLNPDGVFLSNGPGDP
                     EPLHEIAAEVRKLAETYPTFGICLGHQLLALAFGAKTYKLKYGHRGGNHPVKNLVTGR
                     VEITAQNHGFAVEPASMPADFETTHINLNDQTLEGMRHRHLPVYSVQYHPEASPGPHD
                     ASYLFDDFTRLMQQQRTLRTSNVGCEA"
BASE COUNT          321 a          312 c          351 g          299 t
ORIGIN      
        1 gcccatctga cgcgccccct tcacaagcac aatgctgtct ccgcgtgaga tagccgtacc
       61 gaattcttcg tccatccggc gcaaaatata atattttgag tttaatggca ctgcaccgcc
      121 aactggctca agcggttggg gtggtagctc tgatgggatt acaaccgggc cagccgggtt
      181 taagatcgaa agcaccgcct gggtcagtga aagattgtcc tgtgggctca tccacgaaaa
      241 gataatcggc tctggcaaaa tgccggtcag aggtcgaggc agcggatcaa gaaaattgag
      301 tcgaatgggc aaaactttgg gttttccctg ttgttgcgcg gtttcaaacg caatccgaat
      361 ctcataggcc agagtttcac cgctgactga ggtgcccgac aacaacacaa tcactacatc
      421 agccgtgcgg acctgattct tgatttcgcg cacccactcg atcccatcgg tctgagatcg
      481 gtcaataaac acctggcagc cctgggcggt gagcgccgat tctaaaatct gggccacata
      541 gtcatcaggc tgggattgtc gttcgtgaag aatggcaatg tgcataggtt agggttcagg
      601 gttcagggtt cggggtttgg ggtttgggaa atcaaagcaa aaggacaaaa ggacgaaaag
      661 gacgaaaagg acataaattc gaaaacccgg aagcgcggaa gcgcggaacc ctttgtttta
      721 tgcttcacat ccgacgtttg acgtgcgcaa ggtccgctgc tgctgcatca agcgggtgaa
      781 atcatcaaat aaatatgagg catcatgcgg accgggggaa gcttcggggt gatactggac
      841 gctatagacc ggcaggtgac ggtgacgcat gccttccagt gtctggtcat tgagattgat
      901 atgggtggtt tcaaaatcgg cgggcattga tgcgggttca accgcaaacc cgtgattttg
      961 agctgtaatc tcaacccgcc cggtgaccag attcttgact ggatggtttc cgccacgatg
     1021 gccatatttc aatttgtagg ttttggcgcc aaacgccagc gccagcaatt gatgccccag
     1081 acaaatacca aaagttggat aggtttcagc cagtttgcgc acttcagccg caatttcatg
     1141 gagcggttcg gggtcgccag gcccatttga caaaaacaca ccgtccgggt tcagtttttg
     1201 gacttcttca aaagtggtgg tcgccggaac caccgttacc cggcacccaa ttttggttaa
     1261 gtgcctcaaa atattaaatt tga
//
LOCUS       DAOQDO010002213         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132533_length_1282_cov_3.122249, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002213 DAOQDO010000000
VERSION     DAOQDO010002213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132533_length_1282_cov_3.122249"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1282)
                     /locus_tag="PKZ53_26110"
     CDS             complement(<1..>1282)
                     /locus_tag="PKZ53_26110"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNJ43984.1"
                     /translation="VGNDLFVATENGVFQIQGTTATKVRGPNENAPAYCFCPSTRYPD
                     RIYVGQRYGVSVLIRKGSIWTEVGKIPDIDTRVRFISELADGSLWLGMNADGVIKVEM
                     GSSPIPESFNPATAPLTIRRFTTSDGLPMMTRIRVVKMSGKILFFSPSGTFEFDEATH
                     RFSPSTFLSEYLTDSIQFHLSPFLTNAIIHPVQDHLISFLRKPDGQSLQQLVVDRTGT
                     LWGHFHNDVCFRLKPVSGGYQVDLESLKRIPAMGFTTLVADSHNMIWFGGTQGLIRVD
                     PRQIKTPPPPISVLIRKILAGNTQELIFGGTTDMGDISTIELPYAANRLRIECALPVF
                     IEEQAIRYQYFLEGSDAGWSEWTTETVKEYTNLHEGQYRVRIRAEDALGQPSLETACE
                     FQILPPWYRTWWAYLGYLAGFAWAGMGIIRWRVQT"
BASE COUNT          323 a          320 c          343 g          296 t
ORIGIN      
        1 gtgtctgcac gcgccagcga atgataccca ttccggccca ggcaaaaccg gcgaggtaac
       61 ccagatacgc ccaccaggtt cgataccagg gcggcagaat ttgaaactca caggctgttt
      121 cgaggctggg ctgaccaagc gcgtcttcgg cacgaattcg aacccggtac tggccttcgt
      181 gcaaattggt gtattccttg acggtttcag tcgtccattc tgaccagcca gcgtctgagc
      241 cttcaagaaa gtactgatag cggatggcct gttcctcaat gaagaccggc agagcacatt
      301 caatacgcag gcggtttgcc gcgtagggaa gttcaatcgt ggaaatatcg cccatgtccg
      361 ttgtgccacc aaagatcagt tcctgagtgt tcccagccag gattttccga atcagcacgg
      421 aaatgggcgg aggtggtgtc ttgatctggc gtggatcaac ccgaatcaac ccttgtgtgc
      481 cgccaaacca gatcatattg tgtgaatcag ccaccagcgt tgtgaagccc atggctggaa
      541 ttcgcttcaa gctttcaaga tcaacctgat agccaccgct gactggtttg agccgaaaac
      601 aaacatcatt gtgaaagtgc ccccagagcg ttccagttct atcaaccacc agttgctgaa
      661 gcgattggcc atctggcttt ctcagaaaag agatcaggtg atcttgaacc gggtggatga
      721 tggcatttgt caaaaacggg gacaggtgaa actggatgga gtcagtcaaa tattcagaga
      781 gaaatgtcga gggagagaat cggtgggtgg cttcgtcaaa ttcaaacgtt ccgctcggag
      841 aaaaaaacaa aattttgcct gacattttta cgacccgaat ccgggtcatc atcggcagtc
      901 catcactggt ggtaaagcga cggattgtga gtggggcggt ggccgggttg aagctctctg
      961 gaattggaga cgatcccatt tcaaccttga tgacgccgtc tgcattcatt ccaagccaga
     1021 gtgaaccatc cgcaagctcg ctgataaacc gaacccgggt atcaatgtcc ggtattttgc
     1081 cgacttcggt ccaaattgag ccttttcgaa tcaaaactga aaccccgtac cgctgaccga
     1141 catagatgcg atctggatat ctggttgacg ggcaaaaaca gtaggcaggt gcgttttcgt
     1201 ttgggccacg aactttggtg gcggttgtac cttggatctg aaaaacgccg ttttcggtgg
     1261 caacaaacag gtcattgccc ac
//
LOCUS       DAOQDO010002214         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132546_length_1282_cov_2.788101, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002214 DAOQDO010000000
VERSION     DAOQDO010002214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132546_length_1282_cov_2.788101"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKZ53_26115"
     CDS             <1..234
                     /locus_tag="PKZ53_26115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43985.1"
                     /translation="KVVKTALPKPEKPALETLCPPESLELLPPEQLAAQLKQKYSVSE
                     LIELCACLIGSENASLFAAVLSRLRERNAGRVL"
     gene            723..>1282
                     /locus_tag="PKZ53_26120"
     CDS             723..>1282
                     /locus_tag="PKZ53_26120"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNJ43986.1"
                     /translation="MSQFQKNQHSGRYGRLIRLHMTLIRSTLELVKTKLSESLRTEGM
                     TNEDWALLSNLTDQEGQLYEETKHKIVMVLVNIQHETIISTYNRTVPAKGDQYTVIAP
                     PLYIDLFVLLYANFYNQSYRDGLGMISRTISFFQQNPWFTHDNLPGLDPVIDKLTFEM
                     VNLSLPDLNYLMGMLGVKYLPCVYYKV"
BASE COUNT          317 a          316 c          294 g          355 t
ORIGIN      
        1 aaggttgtga aaaccgcact gccaaaaccc gaaaaaccag cgcttgaaac tctgtgccca
       61 cccgagtcgc ttgaactttt accacctgag caactggcag ctcagctcaa gcagaaatat
      121 tcagtgtctg aactgattga gttatgtgcc tgtctgattg gatcggagaa tgcctcgctg
      181 tttgccgccg tactcagtcg tcttcgagaa cgcaatgccg ggagggtgct ttaaccaggc
      241 aactaggcac aatgcgtaga ttgattgatt ccctggattg tcagaaataa ttttgattcc
      301 gatcaagatt gttgcctaac agctacgtgt gtgttatata ggctgggttc gcactgattt
      361 cgtttcaagt ttcgtcaatt tcccatttcc cattcttcaa ttcaattccc ggcacggctg
      421 agtttgtgtc cgtgactgat tctgtaacat ctggttttcg gagtggattt ttgttccttc
      481 cgtgtctttg tctccattta acttacttat gacaaggagc gtccagaccc gtctgagccg
      541 aagattccat aaaaattgaa cacttttcaa cccactgtca gtcattgagg ggggcgccca
      601 accttgctgc cagccgcctg tttttcggtc gtggtgtgac gtgcctgata ccacgtgaag
      661 gtgtgccggt cgtggcgaga tcccaatcag ttttcccctg tacttgattg aacggaagaa
      721 gtgtgtctca attccaaaag aatcaacatt ctggcaggta cggaagactc atccggcttc
      781 atatgacgct gattcggtca accctggaac tggtcaaaac caaattgagc gagtcgcttc
      841 gaacggaagg gatgaccaac gaagattggg cattgctttc aaacctcacc gatcaggaag
      901 gtcagcttta cgaagagacc aaacataaaa tcgtgatggt acttgtcaat attcaacacg
      961 agaccattat cagcacctat aaccgaacgg ttccagccaa aggagatcaa tatactgtga
     1021 ttgctccgcc actctatatt gatctgtttg tgctgctcta cgctaacttt tacaaccaga
     1081 gctatcgcga tggactgggg atgatttctc gcaccatcag cttttttcag caaaatccct
     1141 ggtttaccca tgacaacctc cctggtctgg acccggtgat tgacaagctc acgtttgaaa
     1201 tggtcaacct gagtcttcca gacctgaatt acctgatggg gatgctcggc gtgaaatacc
     1261 tcccctgtgt gtattacaag gt
//
LOCUS       DAOQDO010002215         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132553_length_1282_cov_2.691932, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002215 DAOQDO010000000
VERSION     DAOQDO010002215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132553_length_1282_cov_2.691932"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..187)
                     /locus_tag="PKZ53_26125"
     CDS             complement(50..187)
                     /locus_tag="PKZ53_26125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43987.1"
                     /translation="MKILLDTHTWLWFVLGDLQLSDKARALISDPKNEKLISPASYWE
                     L"
     gene            complement(184..414)
                     /locus_tag="PKZ53_26130"
     CDS             complement(184..414)
                     /locus_tag="PKZ53_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015199289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43988.1"
                     /translation="MSQTVTLEEAQARLPELIARLSPGDDLVILQNERAIAHLQLISE
                     EKPQPVFGSCKGKLIMVSEDDSHLEDFNDYMP"
     gene            complement(574..>1282)
                     /locus_tag="PKZ53_26135"
     CDS             complement(574..>1282)
                     /locus_tag="PKZ53_26135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43989.1"
                     /translation="DAPNVTTLRGKLGSKAEVEKAPLQWVDADTLALEIPLQGTETTL
                     STVDIAGIGKVTLPPVVLPYSPEFRPIASVEGITVLEQLAKATGGTERLDLTSVWKDL
                     PKRPRLIDLTPWLLLTTILLLLVEVLERRTRLVSAQWASLKIGKFHWPKFERLRTPKP
                     KTQLSQPGVVPAHVAPSATPAAPQPSTSVPKFDPKPDPTGPSSLPTDQKDKTAMVEAL
                     RKARQQVRGRQGDTGKK"
BASE COUNT          298 a          312 c          322 g          350 t
ORIGIN      
        1 ttgatgccgg caagatgccg gcgctcccag gaaatattta tctgaaaagc tatagttccc
       61 agtaagaagc cggactgatt aacttttcgt ttttgggatc agaaatcagt gctctggctt
      121 tgtcactcaa ttgtaaatcg ccaaggacaa accataacca ggtgtgggta tcaaggagaa
      181 ttttcatggc atatagtcgt tgaaatcttc cagatgtgaa tcatcttcag acaccatgat
      241 aagcttccct ttgcaacttc caaagactgg ttgtggcttc tcttcggaaa ttagctgtaa
      301 atgagcaatt gcccgttcat tttggagaat gaccagatca tcacctggac ttaacctggc
      361 aattaactct ggtaacctgg cttgagcttc ttcgagggtg acagtttgcg acatggcttg
      421 agctttcctt catggtgtta aaaccgtttg gttatcttaa gtcccttacc gaaaattccc
      481 agacattttg aaccacgaaa tacacaaaat acacgaaaaa aaccaacccc ttccaacatt
      541 caatcactca ggaaactttc gacaaggtac ttatcacttt ttcccggtgt ctccctgtct
      601 gcctcgtacc tgctgccggg ctttgcggag ggcttcgacc atggcggttt tgtctttctg
      661 gtcagtcgga agtgatgatg gcccggttgg atcaggtttt ggatcaaatt tcggaacact
      721 ggttgacggt tgcggcgcgg ctggtgtcgc agatggtgca acgtgggctg gaaccacacc
      781 tggttggctg agttgagttt ttggttttgg cgtccgcaat cgttcaaatt tcggccagtg
      841 gaatttccca attttcaggc tggcccactg ggccgaaacc agtcgggttc gccgttccag
      901 gacttcgacc agcagcagca ggatggttgt cagcagcagc cagggtgtta aatcaatcag
      961 acgcgggcgt ttcgggaggt ccttccagac agacgttaaa tccaatcgtt cggtgccacc
     1021 tgtggctttg gcgagttgtt cgaggaccgt gatgccttca actgacgcaa tcgggcgaaa
     1081 ctcaggtgaa tagggcaaca cgactggtgg caacgttact ttgccaattc cggcaatatc
     1141 caccgtcgaa agtgttgttt ccgtgccctg aagcgggatt tcaagcgcca gggtatcggc
     1201 atcaacccat tggagcggtg ccttttcaac ttcggctttt gaccccaatt ttccacgcaa
     1261 ggtggtcaca tttggcgcat cc
//
LOCUS       DAOQDO010002216         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132587_length_1282_cov_2.284434, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002216 DAOQDO010000000
VERSION     DAOQDO010002216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132587_length_1282_cov_2.284434"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1282)
                     /locus_tag="PKZ53_26140"
     CDS             complement(<1..>1282)
                     /locus_tag="PKZ53_26140"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ43990.1"
                     /translation="GDYNSSLDVFLVQFCPTVTATISGGGPVCAGSSTTVTATILGGT
                     SPYTVTLTSGGGTQTGSSPLIFPVAPGSTTTYALLSGTDANGCPITGNGNVLVMVAPP
                     PTVNAGPDQTVCTSSLPVTLGGAIGGSATVGTWSGGGGSFNPGPTALNARYMPTASEI
                     AAGMVTLTLTTSDPGGVCSPVSDTVTLFILNCAETGQLLVADTANNRIQRFDGTNWSL
                     IGSGVVGSGAGQFRLPEAVTFNGAGRIYVADTGNNRIQWSTDDGATWAIFATVGSASN
                     QVRSPQGVCLDSAGNLYVSDTGNGRVLRFNSGTPGAGVVIASNGAASGQVGSPHGLVI
                     DNTFRLFVTDESNSRILSIASANTVTTSTSGTIIASQGTGLNKVKNPQGITIDQAGAL
                     YIADTGNSRILRWVNANPNNSSALAVTGSLLGQVN"
BASE COUNT          292 a          375 c          333 g          282 t
ORIGIN      
        1 gttgacctgt cccagaagcg atccggtcac tgccagtgct gaactgttgt ttggattggc
       61 attcacccag cgcagaattc gcgaattccc cgtgtcggca atatagagtg ccccggcctg
      121 gtcaattgtg atgccctgcg gatttttgac tttgttgagt cccgttccct gagaggcaat
      181 gatggtgccg ctggtcgaag tcgtcacggt gttggcactg gcaatactca ggattcggct
      241 attactttca tccgtcacaa acaaccgaaa cgtgttgtca atcaccagcc cgtgtggact
      301 gccaacctgc ccgctggcgg cgccgttgct ggcaatcacc acgccagctc caggagttcc
      361 actgttaaag cgtaagaccc gcccatttcc ggtatccgac acatagagat ttccagccga
      421 atccagacag actccctgtg gcgaccgaac ctgattggag gcagaaccaa ccgtggcaaa
      481 aattgcccag gttgcgccat catccgttga ccactggatc cggttattac cagtatccgc
      541 aacatagatt cgaccggcgc cattaaacgt aacggcttct ggcaagcgga attgaccggc
      601 cccagaaccg acaacgcctg agccaatcag actccagttt gttccatcaa agcgttgaat
      661 ccggttattt gccgtatcgg cgaccagtaa ctgaccggtt tcagcacaat tcaggataaa
      721 gagtgtcacc gtatctgaaa ccggcgaaca aaccccgcct ggatcactcg tggtcaatgt
      781 caaggtaacc attccggcag caatttcact ggcggttggc atgtagcggg cattgagtgc
      841 cgtcggtcca gggttaaatg acccaccacc accgctccag gttccaaccg tcgcgctacc
      901 gccaattgcc ccacccaggg tcactggcag gctgctggta caaacggttt ggtctggccc
      961 ggcattgacg gtcggaggcg gtgcaaccat caccaataca tttccattgc cagtgatcgg
     1021 gcatccgttg gcatcggttc ctgacagcag ggcataggtt gtggtgctgc caggtgccac
     1081 cggaaagatc aaaggactgc ttccagtttg ggtaccgccg ccactggtca acgtcaccgt
     1141 atagggtgag gtgccaccca ggatcgtcgc cgtaacagtg gttgaacttc cagcacagac
     1201 cggtccgcca ccgcttatcg ttgcggtaac ggttgggcaa aactgaacca gaaatacatc
     1261 taaacttgaa ttgtaatctc cc
//
LOCUS       DAOQDO010002217         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132724_length_1281_cov_3.050571, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002217 DAOQDO010000000
VERSION     DAOQDO010002217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132724_length_1281_cov_3.050571"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..>1281
                     /locus_tag="PKZ53_26145"
     CDS             423..>1281
                     /locus_tag="PKZ53_26145"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNJ43991.1"
                     /translation="MPVSRQSPVIHSVYVVESLHCLLRRGFHRKLLLLDLLLDFGGNQ
                     SMQAKLRRVSTTVLLIVCLIVIAGQVSAATIPGRSQLILKFRHQVLELINAERKEHGL
                     APLELDQFASTVAQTHAEELSRENFSSHWSRDGRKPYMRYAFAGGTDGAAENISSLFG
                     AKMTAEDPALGEYLNDMHRSLYEETPPNDGHRQTILKPQNTHVGIGIAVLNGQISLVE
                     EFLCRYTVFQPLPAMKMTQKASVSLTGKLTRADSKLRGIEVYYEPLPTPPDLAWLRIS
                     RPYGLPDKHT"
BASE COUNT          329 a          314 c          303 g          335 t
ORIGIN      
        1 ggtgagtggc tgtccatcaa acgggcaaaa atcaacccgc tcgttccatt cccgattaca
       61 ttctgaacac gtcctcataa gcacttttta cctcttgctc gaagaattcc actacattaa
      121 aggatggcgg gggcgttttt cttttgattc aatgtttagc tcctgacttt gtttggccag
      181 gtagctcaat caaaaaggcc gttttctctg gcatcacagc tttttttgag gcaacacata
      241 tctggcactg gtcaaaccgg agtatggttg ccgaatattt ctggaaaaat atatctttgg
      301 gctgaaaaaa tggaaaaata cgcaatgttt tattctacag ataatttcag tctttttttt
      361 taaatttcct ggaaatgtgg gtggaaagac ctagaaaaaa aattttcggc cagtggattg
      421 ctatgccagt cagtcgtcag tcgccagtta ttcactcagt ttatgtggtt gaatcacttc
      481 actgtttgct cagacgagga tttcatcgca aactgctatt actggattta ttactggatt
      541 ttggagggaa tcagtctatg caggctaaac tcagaagagt ctccacgaca gtgttgctca
      601 tcgtgtgctt gatagtaata gccgggcagg tcagtgcagc caccatccca ggcagatccc
      661 aattgatcct gaaatttcga caccaggttt tggaattgat caacgccgag cgcaaagaac
      721 atggcctcgc tccgcttgaa cttgaccagt ttgcctcaac cgttgcccag acccacgcgg
      781 aagaattgtc acgcgaaaat ttttcgagtc actggagtcg cgatggccgc aaaccctata
      841 tgcgctatgc ttttgccggt ggcacagacg gagcggctga gaatatttcg tcgttgtttg
      901 gggccaaaat gacagccgag gatccggcgc tgggcgaata tttaaatgac atgcaccggt
      961 cgttgtatga ggaaacaccc cccaatgatg gccaccgcca aacgatcctg aaacctcaaa
     1021 acacccatgt cggcatcgga attgccgtcc tgaacgggca aatctcgctg gtcgaagaat
     1081 ttctctgccg gtacacggtt tttcagccat tgccggcaat gaaaatgacc cagaaggctt
     1141 cggtttcact gacgggaaag ctgacccgag ccgattccaa actgcgaggg atcgaggtgt
     1201 attatgaacc gctgccgaca ccgccagacc ttgcctggct gcgaatttcg cgcccgtatg
     1261 gcctgccaga caaacacacc a
//
LOCUS       DAOQDO010002218         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132780_length_1281_cov_2.340131, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002218 DAOQDO010000000
VERSION     DAOQDO010002218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132780_length_1281_cov_2.340131"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1171)
                     /locus_tag="PKZ53_26150"
     CDS             complement(164..1171)
                     /locus_tag="PKZ53_26150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ43992.1"
                     /translation="MRTIHTAILWALIGSAGLSIPMVLPARSVQAQDIQNSEEAQARM
                     QKGISLYQQSQYAAALTELQFVAERYPNYGAAYRLAGLCQLQLQQYEPAVVSLKRANE
                     LSMEQEKREDSAARIALSRALFYAGKFEEAIPELTYAVSQKPNDATSHYLLGATHFQL
                     KHEPEALASLSQAVTLNPKDLASWKLLTGIHLQRLAANPEDKTQLTKTQSTLQSLKSL
                     DASAESAGLAGQFYLLTRQFAKAAPELEKAATASPDNGLIQFNLGIALSRSDQFAKAA
                     PVLIRASELLPENTNVLKELGYVYEKDGKAEKARAVYEKGDQLANGQDEYFKTALERV
                     K"
BASE COUNT          252 a          316 c          350 g          363 t
ORIGIN      
        1 tgacctgatc aagttcatgg ctgccaaagg actgtaaatt aataaggtga tagggtttcg
       61 ggtttcgggt tccgggttcc gggttccggg ttccgggttc cgggttttcg atccctatca
      121 cctattctcc atcccctatc ctccatcccc tattctccat ccgctatttc acgcgttcca
      181 atgcggtttt gaaatactca tcctgcccgt ttgcaagctg gtcacctttc tcatacaccg
      241 cccgcgcttt ttcggctttg ccatcctttt catagacata gcccaattcc ttgagcacgt
      301 tggtgttttc gggcaggagt tcgctggctc gaatcaacac tggcgccgct ttggcgaact
      361 ggtcgctgcg cgaaagggca atccccaggt taaattggat caggccgtta tcaggagacg
      421 ccgtggccgc tttttcaagc tccggtgcgg ctttggcaaa ttgtcgggtg agcaaataaa
      481 actgtccggc cagcccggcg ctttctgccg atgcgtccag ggatttcaga ctctgaagag
      541 ttgattgggt tttggtgagt tgggttttat cttccgggtt tgcggccaat cgctgtaaat
      601 gaatacccgt caagagtttc cacgaggcaa gatccttggg gttgagggtt acggcttgtg
      661 acagactggc cagggcttca ggttcgtgtt tgagttgaaa gtgtgtggct ccgagcaggt
      721 aatggctggt ggcgtcgttt ggtttttgag aaacggcgta ggtcaattcc gggatggctt
      781 cttcaaattt accggcataa aacaatgccc ggctcagggc gatccgggca gcggaatctt
      841 cgcgcttttc ctgttccatt gaaagctcat tggcccgctt tagcgacacg acggctggct
      901 catattgctg taactgtaac tgacacaatc cggccagccg ataggcggca ccataattcg
      961 gatagcgttc ggcaacaaac tgcaattcgg tcagtgccgc tgcatactgg ctttgttgat
     1021 acagggaaat gcctttttgc attcgcgcct gggcttcttc actgttttga atatcctggg
     1081 cctgaactga gcgtgctggc aacaccatcg gaatgctcag ccctgcgctg ccaatcagtg
     1141 cccagagaat tgctgtgtgg atcgttcgca tgttgaaaat atttctcctt gtgtggagtc
     1201 agtagtcagt cgttgatgag tcagtagtca gtcgtcagtc gataaaaccc ggatattgta
     1261 gtgtgtggag ttcttgaaaa g
//
LOCUS       DAOQDO010002219         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132976_length_1280_cov_2.507755, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002219 DAOQDO010000000
VERSION     DAOQDO010002219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132976_length_1280_cov_2.507755"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKZ53_26155"
     CDS             <1..237
                     /locus_tag="PKZ53_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43993.1"
                     /translation="IKGETPFLTDENEQQVITKRITLLKKMLLQVHGQVELVSIINLG
                     IICRLATSSATTELALELMVKILPLPFQLSSQKT"
     gene            249..>1280
                     /locus_tag="PKZ53_26160"
     CDS             249..>1280
                     /locus_tag="PKZ53_26160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNJ43994.1"
                     /translation="MDDPGFFASGIFVNKGDQVYGNIIQGFGTTGIHIRDWSGSDVPN
                     INAETVVRDNIILNSQVGIQVGGITAPTFITNNRIENCSKGGIVVCNSTRHPQSIFRY
                     GGTNRTDDAESQVNVPTENVLISQNQFVNVGGPGIALTSFTRSVVDPDGGYQPNLRTI
                     EDPAGPNRYQPSVQLRKAERKTDGSVVLTGTATSPGTLEIYASIRPALIQAPATDPMA
                     YGLGQFLVSIPVSLGEFTVTIPSSQAQNRDAFTATITVGRNTSEFARTIGFSTPDPDP
                     DTQKPVVQVLTPVSGLTVESQVGAQIAVTWTSSDNIGVTEQSITLQGKRQGVLFDQTI
                     ATGLPAATTS"
BASE COUNT          343 a          336 c          310 g          291 t
ORIGIN      
        1 ataaaagggg aaaccccttt tcttacagat gaaaatgaac aacaagtaat taccaaaaga
       61 ataacattat tgaaaaaaat gttattgcag gttcacgggc aggtggaatt ggtgagtata
      121 ataaatttgg ggataatctg ccgattggca acatcatccg caacaaccga attggcattg
      181 gagttaatgg tgaaaatatt gcccctcccc ttccagctca gttcccagaa aacttaggac
      241 aaccaggtgt ggatgatcct ggtttttttg ccagcggcat ttttgtcaac aagggcgatc
      301 aggtttacgg caacatcatc caggggtttg gaaccaccgg gatccatatt cgcgattggt
      361 caggatcaga tgttcctaat atcaatgctg aaaccgtggt tcgagacaac atcattctca
      421 attcccaggt gggaatccag gttggcggca tcaccgcacc gaccttcatc accaacaacc
      481 gaattgaaaa ctgctccaaa ggcggcattg tggtttgcaa tagcacccgt catccccaaa
      541 gcatttttcg atatggggga acaaaccgga ccgatgatgc tgaaagtcag gtgaatgttc
      601 caactgagaa tgtcctgatt tcccagaacc agtttgtgaa cgttggagga cctggaattg
      661 cactcacttc atttacccga agtgtggtgg atcccgatgg cggatatcag ccaaacctgc
      721 gcaccattga agacccggct ggcccaaacc gctatcagcc gtcagttcaa ttgagaaagg
      781 ctgagagaaa aacggatggt agtgtggttc tgactggaac cgccacctca ccaggtacac
      841 tggaaatcta tgcctcaatt cgaccagccc tgatccaggc tccagcaacc gacccgatgg
      901 cttacggcct cggccaattt ctggtcagta tcccggtttc gctgggagag ttcacggtaa
      961 cgattccatc aagccaggct cagaaccggg atgccttcac cgcgacgatc accgttggcc
     1021 gcaacacatc cgagtttgcc cgaacgattg gtttctcaac ccctgacccc gatcccgata
     1081 ctcagaaacc ggtggttcag gtcttgacgc cggtctcagg actaaccgtg gaaagtcagg
     1141 ttggtgccca gatcgccgtg acctggacct ccagcgacaa cattggtgtc accgagcaat
     1201 ccattactct tcaaggaaaa cgtcagggag tgctgtttga ccagaccatt gccactggat
     1261 tgcccgccgc gaccaccagt
//
LOCUS       DAOQDO010002220         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_132987_length_1280_cov_2.380408, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002220 DAOQDO010000000
VERSION     DAOQDO010002220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132987_length_1280_cov_2.380408"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKZ53_26165"
     CDS             <1..357
                     /locus_tag="PKZ53_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011328389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNJ43995.1"
                     /translation="EVILQRLELPYRKVVLATGDMGAASAKTYDLEVWLPSQKTYREI
                     SSCSNFEAFQARRASIRFRREAGAKPELVHTLNGSGLAVGRTWLAILENYQQEDGSIE
                     IPRALRPYLGGLEVIG"
     gene            complement(491..>1280)
                     /locus_tag="PKZ53_26170"
     CDS             complement(491..>1280)
                     /locus_tag="PKZ53_26170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ43996.1"
                     /translation="VQPPPVKSTPVQSESDKPFNPEAAERAMLSRMSLLEQTRYYIMK
                     DQDEFPNFIVKQNIRRLKNKGGGWYEDDVLGTEITYAQGEGEKVKLLTINNVPTTKTF
                     VGVGGTTSIGEFSSRLLSLFQLKSKTDFKEVGRENYRGRECTVFDFSVAEENSNYVIE
                     GTGPDGQRQQINSAYEGRLWVDTTSKRVLRIEQFAVQMPRDFLFSQAETAVEYDWIEI
                     GEKQYWLPISAENQIGSDRYDYHTRNIIKFYDYRKFEAEVKILD"
BASE COUNT          295 a          319 c          320 g          346 t
ORIGIN      
        1 gaagtgattt tgcagcggct ggaattgccc taccgcaagg tcgtgctggc gacgggcgat
       61 atgggcgccg catcggccaa aacctatgac ctcgaagtct ggttgccaag tcagaaaaca
      121 tatcgcgaaa tttcgtcctg ctcaaacttc gaagcctttc aggcacgccg ggcgagtatc
      181 cggtttcgtc gtgaagccgg ggccaaacca gaactggtcc acacgctcaa tggctcaggt
      241 ttagccgtcg gtcggacgtg gctggcgatt ctggagaatt atcagcagga agatggctcg
      301 attgaaattc cacgggccct ccggccatat ctgggcgggt tggaagtgat tgggtaggaa
      361 cgtcgggctg ggggctgaag acatcgggct gaagactcgc aagctcgggg cggaagaaaa
      421 tgggtttaat accccaagcc cgttttcttc agccccaagc ccgctttctt cagccccggt
      481 tttttcagtc ctaatccaaa atcttcacct ccgcttcaaa cttgcggtag tcataaaact
      541 taatgatgtt ccgtgtatgg taatcataac gatcactgcc aatctgattt tcagcgctaa
      601 ttggtaacca gtactgcttt tctccgatct cgatccaatc atattcgaca gcggtttcag
      661 cctgagaaaa caaaaaatcg cgaggcattt gaacggcgaa ttgttcaatt cgcaacaccc
      721 gctttgacgt ggtatcaacc cacaggcggc cttcataggc tgagttgatt tgctgccgtt
      781 ggccatcggg gcctgtcccc tcaattacat aatttgagtt ttcttctgcc acggaaaaat
      841 caaaaaccgt acactctcgt ccgcgataat tttcccgccc gacttcttta aagtcagttt
      901 tgctttttaa ttgaaacaaa gataagagcc gtgaagagaa ctcgccaatt gaagtggtcc
      961 ccccaacgcc gacaaaggtt ttggtggttg gcacattgtt gatcgtcagg agtttgactt
     1021 tttcaccttc accttgtgcg taggtaattt ccgttccaag gacatcatct tcataccagc
     1081 ccccgccctt gtttttgagc cgccgaatgt tttgtttcac aatgaaattt gggaattcat
     1141 cctggtcttt catgatgtag tagcgcgttt gttccagtag cgacatccga ctgagcattg
     1201 ctcgttcagc cgcttcaggg ttgaagggtt tgtcggattc ggattgaact ggtgttgatt
     1261 tcacaggtgg aggttgaact
//
LOCUS       DAOQDO010002221         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_133013_length_1280_cov_2.140408, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002221 DAOQDO010000000
VERSION     DAOQDO010002221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133013_length_1280_cov_2.140408"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PKZ53_26175"
     CDS             <1..1156
                     /locus_tag="PKZ53_26175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ43997.1"
                     /translation="KSNKKIVITRVTTDITEDGKQAKSEYTVVHPEGTPGHQVTLPRI
                     QARIVEAEATPDRLIVISGAPGGEKRIPLPTSRSTQVIQTTLQQGLIAGILLLLAGFF
                     ASAMIAHRLTSPLRQLMAGVEALERGQLGTQIQVTSKGELGELQTTFNRMSARLAELE
                     EEKQTWKAREHLAELGDLARGLAHTLRNPLNTLGLAVEELASNTADGSQDLVTTARGQ
                     IRRIDHWLRSFLALGAGGAAEPDIVDIRELVQDVAFEAIQLGHDIEVTSCSEPLKALV
                     VPPALRSALANLMNNAVEATPDHSPVTVTVTRVEHTGLIRIIDRGRGLPDEVRRRLFT
                     PHITTKTGGSGMGIFLAKQLIEGGHGGKLEFQDNPDGGTIALISIPLNIK"
BASE COUNT          304 a          329 c          348 g          299 t
ORIGIN      
        1 aaaatccaat aaaaagatcg tcattacacg agtcacaacc gacatcaccg aagatggaaa
       61 gcaggcaaag tcagaatata ccgtcgtcca cccggagggg acacctggac atcaggtcac
      121 attgcctcgg atccaggcac gaatcgttga agctgaagcg acaccagacc gactgattgt
      181 cattagtggc gctccgggag gcgaaaagcg aatcccgctt ccaacatcac gctcaacaca
      241 ggtgatccaa accacactcc aacaagggtt aatcgcgggc atactgctcc tgctggctgg
      301 tttttttgct tcagcaatga ttgcccatcg gttgacatct ccgctccgcc agttgatggc
      361 tggggttgaa gccctggaac gtggccagct cggcacccaa attcaagtca cgtccaaagg
      421 tgaattgggc gaattgcaaa ccacgtttaa tcggatgtct gcccggctgg ccgaacttga
      481 agaggaaaaa caaacctgga aagcccgcga acacctggcc gaactcggtg acctggcccg
      541 aggattggca cacacacttc gcaatccact caatacgctc gggttagcgg tcgaagagct
      601 ggcctccaat acggctgatg gcagtcagga tctggtgacc acggcccgtg gtcagattcg
      661 tcgaattgac cactggctcc ggtcatttct ggcgttgggt gcgggtgggg cggcggaacc
      721 agatattgtg gatattcgcg agctggttca ggatgtagcg tttgaagcca ttcaacttgg
      781 acatgatatt gaagttacct cctgttctga acccctcaaa gctctggttg tccctccagc
      841 cttacgttct gccctggcga atctgatgaa taatgccgtt gaggccacgc ctgaccattc
      901 cccagtcaca gtgacggtca ccagagttga gcacaccggg ttgatccgca tcatagaccg
      961 tggtcgaggt ctcccagacg aagtccggcg gcgattgttt accccgcata tcacgaccaa
     1021 aactggtggc tcagggatgg gtatttttct ggcaaaacaa ttgatcgaag gcggacatgg
     1081 tgggaaactg gagtttcagg ataacccaga cgggggtacg atagcgctta tctccatacc
     1141 actcaacatc aagtaagaat tgagaatgaa gaatgaagaa ttgatcggtc cctggtgctt
     1201 ggtgcttggt tctttgttct ttgttcttgg ttcttggtgc ttggtgcttg gttcttggtg
     1261 cttggtgctt ggttcttggt
//
LOCUS       DAOQDO010002222         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_133141_length_1279_cov_3.420752, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002222 DAOQDO010000000
VERSION     DAOQDO010002222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133141_length_1279_cov_3.420752"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>1279)
                     /locus_tag="PKZ53_26180"
     CDS             complement(47..>1279)
                     /locus_tag="PKZ53_26180"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ43998.1"
                     /translation="LRSQLNPVTLSSIQKLIPANASLIEFATYRPLTKPQTRSYGPAR
                     YIVYTLDQTGDIKWVDLGECAPIDRAVAEFRQVLSTAKSDPGKEVSPRARKVYELVMK
                     PVTRLVGNHTQLILSPDGELNLLPFGALITEKNKYLIERYSVSYVSSGRDLLRLQNQV
                     RNQEPPLIVADPQYVGEGPMLAGVRANPLQRLIETAEEGKQLQALFPNAVLKLREEAT
                     EDIVKSAKHPSILHLAAHGFFLEESAPDKDRDLLLRSQEPQLSANLQVSSRVNPLLRS
                     GLFLANTSQPGRRQNDGTLTALEMTNLDLTGTKLVVLSACNTGVGDIKNGAGVYGLRR
                     ALVLAGSETQLTSLWKVSDLGTRKLMVDYYTRLKAGEGRSEALRQVQLSMLASPRWRH
                     PFYWASFIQSGQWKPLEE"
BASE COUNT          271 a          344 c          346 g          318 t
ORIGIN      
        1 ttgattgagt gaacttctca attcttcatt cttcattctt cattcttcat tcttccagcg
       61 gtttccattg tccagactgg ataaaactcg cccagtaaaa aggatgccgc cagcgtggag
      121 aggccagcat tgagagttgc acctggcgca gggcttcaga gcggccttca ccggctttga
      181 ggcgggtata gtaatccacc atcagtttcc gcgttccgag atccgagact ttccacagac
      241 tggtaagctg ggtttcactt ccggccagca ccagcgcccg tcgcagtcca tacaccccgg
      301 cgccgttttt gatgtcgccc acgcccgtgt tgcaggccga gagcaccacc agcttggtcc
      361 cggtcagatc aaggttggtc atttcaagtg cagtgagtgt gccatcgttt tggcgccttc
      421 ccggctggct ggtattggcc aaaaataacc ctgaccgcag caacgggttg actctggatg
      481 acacctgtaa attggctgaa agctggggtt cctggcttcg gagcaaaagg tcgcgatcct
      541 tgtctggcgc gctctcttcc agaaaaaacc cgtgggcggc cagatgcaga atagatgggt
      601 gtttggctga tttaacaatg tcttccgtcg cttcctcccg gagcttgagc accgcatttg
      661 gaaacaatgc ctgcaactgt tttccttcct cggcggtttc gatcaggcgt tggagcgggt
      721 tggcgcgaac accagccagc atcgggcctt caccgacata ttgcggatcg gccacaatca
      781 agggcggttc ctgattcctg acctgatttt gcaatcggag caaatcgcgt ccgcttgaaa
      841 cgtacgacac actgtaacgc tcaatcaggt atttattttt ttcagtgatc aaagcaccaa
      901 acggcagtag attgagttct ccgtctggag aaaggatgag ttgcgtgtgg ttgccgacca
      961 gccgggtgac tggtttcatg accagttcgt acactttgcg ggcacggggg gaaacttctt
     1021 tgcctggatc actcttggcc gtacttaaaa cctggcggaa ctcagccact gctcgatcaa
     1081 tcggcgcaca ttcccccaga tcaacccatt tgatatcccc agtctgatca agtgtataca
     1141 cgatatagcg cgccgggccg tagctgcgag tctgcggctt ggtcagcggg cggtaggtgg
     1201 caaattcaat cagtgacgca ttggctggaa tcagtttttg aatgcttgaa agggtcaccg
     1261 gattcaactg ggagcgcag
//
LOCUS       DAOQDO010002223         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_133349_length_1278_cov_3.217498, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002223 DAOQDO010000000
VERSION     DAOQDO010002223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133349_length_1278_cov_3.217498"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKZ53_26185"
     CDS             complement(<1..748)
                     /locus_tag="PKZ53_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ43999.1"
                     /translation="MSTSSAPTSAKIGMIGLAVMGRNLAMNIERNGFPVAVWNRDPGT
                     VDKFMETVEGKQFIGAHSPEEFVKSIERPRKIILLVKAGSPVDWTIDQIKPYLEEGDI
                     IIDGGNSYFPDTIRREKALKAEGLNFIGCGVSGGEEGALWGPSLMPGGDPEAYKNLAP
                     IWEAIAAKVDDGLCVTYIGPDGAGHFVKMVHNGIEYGDMQLIAEAYDILRRVLNMEAA
                     ELAEVFTKWNEGSLQSFLIEITSHIFKAIDP"
     gene            complement(880..>1278)
                     /locus_tag="PKZ53_26190"
     CDS             complement(880..>1278)
                     /locus_tag="PKZ53_26190"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719806.1"
                     /note="catalyzes the reversible formation of D-erythrose
                     4-phosphate and D-fructose 6-phosphate from sedoheptulose
                     7-phosphate and D-glyceraldehyde 3-phosphate; catalyzes
                     isomerization of glucose 6-phosphate and fructose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNJ44000.1"
                     /translation="DRLTDGDYFAVLAYVEMNETHDQPLQTLRHQVRDSKKVATCLGF
                     GPRFLHSTGQAYKGGPNSGVFLQITCDDAADLPVPGQTFTFGVVKAAQARGDFQVLAE
                     RNRRALRIHLGADVKAGLDTLQAAVAAALA"
BASE COUNT          278 a          325 c          365 g          310 t
ORIGIN      
        1 ctggatcaat ggccttaaag atgtgggagg tgatttcgat gaggaaggat tggagcgaac
       61 cttcattcca tttggtaaac acttcggcca gttcggcggc ttccatgttc agaacccggc
      121 gcaggatgtc ataggcttcg gcaatgagtt gcatgtcgcc atattcaatt ccattgtgga
      181 ccattttgac aaaatgtccg gcgccatcag ggccgatata ggtcacacag aggccgtcat
      241 caactttggc ggcaatcgct tcccagatcg gggccagatt tttataggcc tccgggtcgc
      301 cgcccggcat cagggatggt ccccacaggg cgccttcttc accgcccgac acgccgcatc
      361 cgataaaatt cagaccttca gctttgaggg ctttttcgcg gcgaatggtg tcggggaagt
      421 acgagtttcc gccatcaata atgatatcgc cttcttccag atagggtttg atctgatcaa
      481 tagtccaatc cacaggggaa ccagccttga ccaggaggat gattttgcga ggtcgttcaa
      541 tggatttgac aaattcctcg ggtgagtggg cgccgataaa ctgttttccc tcaacagttt
      601 ccatgaattt atcaacggtg ccgggatcgc ggttccagac ggcaaccggg aatccgttgc
      661 gttcaatgtt catcgccagg ttgcgtccca tgacggcgag accgatcata ccgattttgg
      721 cagaagttgg ggcactgctt gtagacatgg cacttttcct cttttcgtag ttgttcgttg
      781 gaaggtgaaa ccaaaattgt gagggatgac tgatgaggaa gcgatttgat cgagtaccag
      841 atggcctgtg cctcatcagt cataattcat cagttaaact caggctaaag ctgccgccac
      901 ggctgcctgg agcgtgtcga gtcccgcttt gacatccgca ccgaggtgaa tgcggagggc
      961 acgccggttg cgctccgcca gcacctggaa atcgcctcgg gcttgagcgg ccttgacgac
     1021 tccgaacgtg aaggtttgtc ctggtacggg caaatcagcg gcatcgtcac aggtgatctg
     1081 gaggaagacc cctgaattcg gaccaccttt gtatgcctgg ccagttgagt ggaggaagcg
     1141 tggcccgaac ccaaggcagg tcgccacttt tttgctgtcg cggacctggt gtcgaagggt
     1201 ctggagcggc tggtcatggg tttcattcat ttcgacatag gccaggacgg caaagtaatc
     1261 accatcggtc aatcggtc
//
LOCUS       DAOQDO010002224         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_133566_length_1277_cov_2.645663, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002224 DAOQDO010000000
VERSION     DAOQDO010002224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133566_length_1277_cov_2.645663"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1109)
                     /locus_tag="PKZ53_26195"
     CDS             complement(129..1109)
                     /locus_tag="PKZ53_26195"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNJ44001.1"
                     /translation="MNSTKLMMTFLIGVLGLLGAGWYSGNTIWAQSAATALDKSAASP
                     SDYLIGPGDTVDVRIFKQPEMSGRFRVSEQGNLNLPFVGVVQAAGQTETELTGVIREK
                     LTRYLKQPELSVTVSEFLSQSVTVVGAVKTTGKYPLRRPERILDVLGMAGGLTETAGK
                     SIQLLRRSPMASSEGALSSEAVELVTIQLADLLTGRPEYNQPLVNGDLISVPEADTIY
                     VTGNVVKPGSFTPRENVTLSQAIALAGGLTPTAQASNISVFRGIPGATARQELVFKLR
                     DIETRKVADPVLLANDVVYIRGSESRSIGYAFLRAITTGFGTAAGYAIFR"
BASE COUNT          318 a          360 c          305 g          294 t
ORIGIN      
        1 tttgaatcag aattcacgat aagaggacaa cccacgaatt gaccagagga ggacctgcag
       61 cttaagaaaa ttataggaag accctgaact ggctgggccg gcatccggca ggttcaccag
      121 atgtgaacct accggaaaat cgcatagcca gccgccgttc caaaaccagt ggtgattgct
      181 cgcagaaagg catagccaat gctgcgtgat tcagaccccc ggatataaac cacatcgttg
      241 gctagcaaaa ccgggtcggc cacttttcgg gtttcaatgt cccgaagttt aaacaccagt
      301 tcctgacggg cggttgcccc aggaatcccg cgaaaaacgc tgatatttga tgcctgcgcg
      361 gttggtgtta agcccccagc cagggcaatc gcctgtgaaa gggtgacgtt ctcgcgaggg
      421 gtaaacgatc ctggcttgac cacattcccg gtgacataaa tcgtgtcggc ctcgggcaca
      481 ctgatcaaat ctccattcac cagcggctgg ttgtactcag gacgacctgt cagtaaatcg
      541 gcaagctgga ttgtcacaag ttcgacagcc tcagacgaaa gagcaccttc agatgaagcc
      601 atcggcgaac gacgcaacag ttgaattgat tttccagccg tttcagtcaa cccgcctgcc
      661 attcccagga catccagtat tcgttcaggc cgtctcagcg ggtacttccc ggttgttttg
      721 acggcgccga caacggtgac cgattgggat aaaaattcac tcacggtgac gcttaactca
      781 ggttgtttta aatatcgggt taatttctcg cggatgaccc cggtcagttc cgtttcggtt
      841 tgaccggctg cctggacaac cccaacgaat ggaagattga ggtttccctg ctcgctcact
      901 cgaaatcgcc ctgacatttc gggttgttta aaaattcgga catcaaccgt gtcacccggc
      961 ccaatcaaat aatctgacgg actggcggct gatttatcca gcgcggtggc ggcagattgc
     1021 gcccagattg tatttcccga ataccacccg gcgcccaaca aacccagcac tccgatcaga
     1081 aatgtcatca tcaatttcgt tgagttcata aatactcctc tcgctcataa accataaagt
     1141 tgggcaccac ctgtcgtgag cccgtgcctg ccgactttga atacaaaact ggtgccacac
     1201 agggctcaac ttccccactt caaccagtct cctatgcctt ttgaggacta aaccgactgg
     1261 taagtctctt ctttctc
//
LOCUS       DAOQDO010002225         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_133583_length_1277_cov_2.415712, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002225 DAOQDO010000000
VERSION     DAOQDO010002225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133583_length_1277_cov_2.415712"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKZ53_26200"
     CDS             <1..236
                     /locus_tag="PKZ53_26200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ44002.1"
                     /translation="QVLIAVCDNGIGITSKQMEHLFKPFNTSKPHGTGLGLVIVKKML
                     VNMRGTIHIESLAQAGTQVTLSLELALESTRTR"
     gene            283..>1277
                     /locus_tag="PKZ53_26205"
     CDS             283..>1277
                     /locus_tag="PKZ53_26205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ44003.1"
                     /translation="MKHVLIVDDEQSFLLSLSEGLAPLQSEFTVHLARNGLEAVAVLY
                     QNKIDLVVTDLKMPEMDGFDLLAHLVGEYPTVPAIVMTAFGTFEIEEQIRDIGAFPYL
                     EKPIELHVLADRILNELDAQNKGKISGVSLPSFLQLIEMEQKTFTLRVSSERRVAYLY
                     FLEGSLIDAETSTLRGEEAIFEILQWVEPEIQINRFCRRGTKVIQNSLSNILLEAMRM
                     LDELNYGLEPNPPAKAELPASSSSSHPAPVFDPLMARSDLPVGSNLDALYQHDRELPI
                     LFTSDPGYPNRVLKSDEDLPSVTQTLGELMGIEGALAVALCDWNARICLGTSGIDS"
BASE COUNT          316 a          305 c          319 g          337 t
ORIGIN      
        1 accaggtttt gattgccgtg tgtgacaatg gaatcgggat cacctcaaaa cagatggaac
       61 atttatttaa gccatttaat acatccaaac cccatggtac cggtctgggg ctggtgattg
      121 tcaaaaaaat gctggtcaat atgcgtggaa ccattcacat tgaaagcctt gcccaggctg
      181 gtacccaggt gacgctttcg cttgaactgg cactggaaag tacccggaca aggtgacacg
      241 aatgcgaacc cctaaccccg ttcgacactt gggtggactc ctatgaaaca tgtactcatt
      301 gttgatgatg aacaatcttt tttattaagt ctttcggagg gcctggcgcc gttacaatcg
      361 gaatttaccg tccacctggc caggaatggg cttgaagccg tggcggtttt gtatcagaac
      421 aagattgatc tggtggtcac tgatttaaaa atgccggaaa tggatggctt tgatctgttg
      481 gctcatctcg tcggtgaata tcccacagtc ccagccattg ttatgacggc gtttggcacg
      541 ttcgaaattg aagaacaaat ccgggacatt ggagcttttc cttacctgga gaaaccaatc
      601 gaactccacg ttctggctga tcgcattctc aatgaactgg atgctcagaa caaagggaaa
      661 atctctggcg tgagcctgcc ctcatttctg caactgattg aaatggagca gaaaacgttt
      721 acattgcggg tctcttcaga gcgaagggtc gcctatctgt actttttgga agggagtctg
      781 attgatgccg aaaccagcac cctgcgcgga gaagaagcca tttttgaaat tttgcaatgg
      841 gttgagccgg aaattcagat caatcgcttt tgccggcggg ggaccaaagt catccaaaat
      901 tcgctctcta atattttgct ggaggcgatg cgaatgcttg atgaactcaa ttatggcctg
      961 gagcccaatc caccagccaa agctgagcta ccagcctcct catcctcatc gcacccagca
     1021 cctgtgttcg atccgttgat ggccagatct gatttacctg tcggatcaaa tctggatgcg
     1081 ctgtatcagc atgatcggga gcttccgatt ctgttcactt ctgatccagg ttaccccaat
     1141 cgagttttaa aatcagatga ggaccttcca tcagtcaccc agacgctcgg cgagcttatg
     1201 gggattgaag gggcgctggc cgttgcactg tgtgattgga atgctcgaat ctgccttggc
     1261 acctctggga ttgattc
//
LOCUS       DAOQDO010002226         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_133800_length_1276_cov_2.624898, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002226 DAOQDO010000000
VERSION     DAOQDO010002226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133800_length_1276_cov_2.624898"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..579
                     /locus_tag="PKZ53_26210"
     CDS             43..579
                     /locus_tag="PKZ53_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44004.1"
                     /translation="MALFVHLTPAKNIRSIRKHGLLLKKNRWFRAAGIFAMPVVPDFV
                     VTHQWVRELRRWGRQEELIAVHFRIPDTEPVLVGHYNEGMKEMTAAEASHLIASTTKP
                     FGYQVLIQRAIRPKELHAIRTVRQVVGWRYRPGSNGTRPCVCMGCNRGQWGIQRLKRA
                     AEKTEKRKQTAKTIVFGR"
     gene            699..1076
                     /locus_tag="PKZ53_26215"
     CDS             699..1076
                     /locus_tag="PKZ53_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNJ44005.1"
                     /translation="MSSTVKKSPRITQISWGHIEVEGLGAGKDFKLFPGGGREWDWNE
                     TGTRHVPGIQPTDVQELLDHGSQVIVLSRGMHLVLQTHPQTLKLLKEKNIQVHVAETK
                     AAVALYNKLAETELVGGLFHSTC"
BASE COUNT          334 a          313 c          345 g          284 t
ORIGIN      
        1 cataaccaat cattttgttg actcgctctt tcgctcaaaa ccatggcgct tttcgttcac
       61 ctcacaccag ccaaaaacat tcgctcgatt cgcaaacacg ggttgctgtt aaagaaaaac
      121 cgctggtttc gagccgctgg gatctttgca atgccggtgg tgccagattt tgtggtcacg
      181 catcaatggg tgagagaact ccgccgctgg ggacgacaag aagaactcat cgccgttcat
      241 ttccggattc cagacactga accagtgctg gttgggcatt acaatgaggg aatgaaggaa
      301 atgaccgccg ccgaagccag tcacctgatt gccagcacca cgaaaccgtt tgggtatcag
      361 gtgttgatcc aacgggccat tcgcccaaag gaactccacg ccattcgcac ggtcagacag
      421 gtcgtcggtt ggcgctatcg tcctggctca aacggcactc gcccctgtgt gtgcatgggt
      481 tgtaaccgtg gccagtgggg aatccagcgg ctgaaacgtg ccgccgaaaa gaccgaaaaa
      541 cggaagcaga cggccaaaac aattgtgttt ggtcggtgaa gactgagggc tgaagaaggc
      601 gggctgaaga aattgggctg agggctgagg gctgaagaaa ttgggctgag gggaaacaat
      661 tctttatcac tgggctggaa agattttcgg agggaactat gtcatcaact gtcaaaaaat
      721 cacctcgcat cacacaaatt tcctgggggc acattgaagt cgaaggactg ggtgctggaa
      781 aagattttaa gctcttccct ggcggcggac gcgagtggga ctggaatgaa accggaacgc
      841 gccacgtgcc gggcattcaa ccgacggatg tccaggaatt actcgaccat ggcagtcagg
      901 tgattgtact ttcacgcgga atgcacctgg tacttcaaac acatccacaa acgttgaaac
      961 tgctcaaaga aaagaatatc caggttcacg tggcagaaac caaagccgct gtggcgctct
     1021 ataacaaact ggctgaaacc gagctggtcg gtggactgtt tcactcaacc tgttgaacaa
     1081 aatcacagct ttaaaagaat ttggagtgct atgacttgtc atagctttgg tttccagcga
     1141 cttgtcgcgc aacggcttcg ttccacgaac gacaagttcg gcgcactcca agaataggta
     1201 tcggtctgtt ctttgccggc caagaatgtt acggctggaa attcttttca aggctctttg
     1261 caacttgccg ggctaa
//
LOCUS       DAOQDO010002227         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_134062_length_1275_cov_2.192623, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002227 DAOQDO010000000
VERSION     DAOQDO010002227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134062_length_1275_cov_2.192623"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1275)
                     /locus_tag="PKZ53_26220"
     CDS             complement(<1..>1275)
                     /locus_tag="PKZ53_26220"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44006.1"
                     /translation="EHNLERNLISGSERQKQQYLKSFVISGDRTISLNIQQAPHDRAA
                     LEIALTLLLQRKGRALDAMANALATLRNQSDHETQKLLDEYTNLVGQISVQTLKGPGQ
                     KKLEDHLTFLKALGDEKEALEKTISRRSAEFKAQTAPVTLEDVQKNIPPDAALIEFVF
                     YQPYLSRAKSPQLGVYVFDHKGNIQCADLGEAKQIEKTVSAFRKVLSTPKTNLAQEIK
                     PIAQELDRLVMKPVRALVGNAKHLLISPDGALNLIPFAALMDEQGKFLIENYELTYLT
                     SGRDLLKLAVKIKSHQPPLIIADPDYRDGTGPQTMERPIGRLTRLIGTQVEGNQIKAI
                     FPEARLKMRAEATEQDFKQVDRPALVHIATHGYFLEDAPQPSETTPADQLDRNIERAT
                     FNFEKERQANPLLRSMLFFAGANQGGTQDNDGV"
BASE COUNT          271 a          309 c          331 g          364 t
ORIGIN      
        1 cacgccatcg ttgtcttgtg tgccgccctg gttggcacca gcgaaaaaca acattgaccg
       61 gagcaaggga ttggcctgtc gttctttttc aaaattgaaa gtggctcttt ctatgtttcg
      121 gtccagttgg tcagcaggag tcgtttccga cggttgaggt gcatcttcca gaaaatagcc
      181 gtgggtcgca atatggacca aagctggccg gtcaacctgt ttgaaatcct gttctgtcgc
      241 ctcagccctc attttcaatc tggcttcggg aaagatggct ttgatctggt tgccttcaac
      301 ctgggtgcca atcagtcgcg tcaatcgtcc aatgggacgc tccattgtct ggggtccggt
      361 tccatcccga taatccgggt cggcgatgat gagaggtggt tgatgggact taattttcac
      421 cgcgagcttc aacaggtcac gcccactggt caggtaggtc aactcataat tttcaatcag
      481 gaatttcccc tgctcatcca tgagggcagc aaatgggatc aggttgagcg ccccatccgg
      541 cgaaatcagc agatgtttgg cgtttccaac cagcgcccgg actggcttca tcaccagccg
      601 gtccaattcc tgggcaatgg gcttgatttc ctgggccagg tttgttttgg gggtgcttaa
      661 cactttgcgg aaagcactga ccgttttttc aatctgtttc gcctccccaa ggtcagcaca
      721 ttgaatgttc cctttgtgat caaacacgta aacacccaac tgaggcgatt tggctctgga
      781 taggtagggt tgatagaaga caaactcaat caaagcggca tctgggggaa tatttttttg
      841 aacatcttcc aacgtgacag gagcggtttg agctttaaat tctgcacttc ggcgactaat
      901 ggttttttcc agcgcttcct tttcatcacc cagggctttg aggaaggtaa ggtgatcttc
      961 cagcttcttt tggccggggc cttttaacgt ttgaaccgag atttgtccga ccagatttgt
     1021 atactcatcc agcagttttt gtgtttcgtg gtcactttgg ttccgcaagg ttgcgagcgc
     1081 atttgccatt gcatccagtg cccgcccttt gcgttgtaag agcagggtca gggcaatttc
     1141 aagcgcggct ctgtcgtgtg gcgcttgctg aatatttaaa gaaatggttc ggtctccaga
     1201 aataacaaac gatttcaggt actgctgttt ttggcgctca gagccggaaa tcaaattgcg
     1261 ctcaaggtta tgctc
//
LOCUS       DAOQDO010002228         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_134663_length_1272_cov_2.453574, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002228 DAOQDO010000000
VERSION     DAOQDO010002228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134663_length_1272_cov_2.453574"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKZ53_26225"
     CDS             complement(<1..528)
                     /locus_tag="PKZ53_26225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44007.1"
                     /translation="MTLTLFTCHAGHLVLPNRTLVLVSRESGGNLLVNPLRTVWERAE
                     LTPVELTLWSFLVAAAGKAMLEVLPQLEGGCINYWEAGNWALNDQAIPPGPKTAPEYR
                     QVHLHLLGRSRTTTDPSWKWGEAPRFPDFVDRHAWAANNERLTPTECQHIVARVEQLL
                     QIRYGLHASQLTPWAT"
     gene            complement(533..1069)
                     /locus_tag="PKZ53_26230"
     CDS             complement(533..1069)
                     /locus_tag="PKZ53_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44008.1"
                     /translation="MPSSRSLILKPPLWACITFPITIVAGFHLLVVYPLFRLISNLDE
                     VSNRTLAVHVVIICFGLWLIGNAGLRMWREVQFSAYLDADGIRVITWRELASFAWSDV
                     IRCELRTRSEFGESGVWITLVGHDNQVLAQWNRNWYRYTRSSLTQFDLFTSQIWAFLG
                     QNEQAQSTIQAEPLPQSE"
BASE COUNT          317 a          318 c          355 g          282 t
ORIGIN      
        1 ggttgcccag ggggttagct ggctggcgtg gaggccgtac cggatctgga gcaactgttc
       61 gacgcgggcc acaatgtgct ggcattcagt gggcgtaagt cgctcattgt ttgccgccca
      121 ggcatgacga tctacaaagt ctgggaagcg gggtgcttcg ccccatttcc acgaagggtc
      181 ggttgtcgtt cggcttcggc caagcagatg cagatggact tgacgatatt cgggcgctgt
      241 tttcggacct ggagggatgg cctgatcatt gagcgcccag tttccggctt cccagtagtt
      301 gatacatccg ccttccagtt ggggcagcac ctcaagcatg gcttttccag ccgcagccac
      361 cagaaacgac cagagcgtca gttcaactgg cgtaagttca gcgcgttccc aaacggtgcg
      421 tagcgggtta accagcagat taccgccgct ttcccgactg acaagcacca gtgtgcggtt
      481 gggcaggacc agatgtcctg cgtgacaggt gaagagcgtg agcgtcattc atttattccg
      541 actgagggag tggttcggcc tggatggtag attgtgcctg ttcattttga ccaaggaaag
      601 cccaaatctg actggtaaag agatcaaatt gcgtcagtga tgaccgggtg tagcgatacc
      661 agtttcgatt ccactgcgcc agaacctgat tatcgtgacc aaccagtgta atccagacgc
      721 cagattcgcc aaattcgctc cgggtccgga gttcacacct gatcacatca gaccaggcaa
      781 agctcgccag ctcgcgccag gtgatgactc ggattccgtc agcatccaga taggcactga
      841 actgtacttc acgccacatg cggagcccgg cattccctat cagccagagt ccaaagcaaa
      901 taatgacgac gtgtactgcc agtgttcggt tggaaacttc atcaaggttg gaaatcagcc
      961 gaaacaatgg ataaaccacc aatagatgaa agccagccac aattgtgatt ggaaaggtga
     1021 tacaggccca cagcggtggt tttaaaatga gagagcgtga tgatggcata ttgaaacaaa
     1081 cagttcaggt gatgaatgaa tgtgaacaat acggtgaaac atctgatggc cgattcgcac
     1141 aaaccagata tccgtcacct cacggcggga agtgataccc tttggggctg agggtatcgg
     1201 gctaagggct gagggaaata cattttgatc agtcacttaa cctctcaaaa atgcgatagc
     1261 ttcattccaa at
//
LOCUS       DAOQDO010002229         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_134928_length_1271_cov_2.051809, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002229 DAOQDO010000000
VERSION     DAOQDO010002229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134928_length_1271_cov_2.051809"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..>1271
                     /locus_tag="PKZ53_26235"
     CDS             166..>1271
                     /locus_tag="PKZ53_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNJ44009.1"
                     /translation="MRLISPPEQGQLVDVCSRRFVVTDVRRTSLPIDPLLNGLPQPQH
                     LITLSSVEDDALGEELQVIWELEPGAQAFERMELPEPSGFDEPARLDAFLDAVRWGAA
                     SSAEMRMLQSPFRSGIEIEDYQLDPVVRAIQMPRVNLLIADDVGLGKTIEAGLVLQEL
                     VIRHRVRRALIICPASLQIQWQEQMRDKFGLEFRIIDGDAMKRLRRTRGIHVNPWSHY
                     PRLITSIDFFKREQPLRLFRATLPHEGELKYPRRYDLLIVDEAHNVAPSATGAYAIDS
                     LRTAAVRLIADHFDHRLFLTATPHNGYSESFTALLELLDPQRFARGISPDRRQLQAVM
                     VRRLKRELPPRWDGTPRFPERKLEALSVAYSEQE"
BASE COUNT          265 a          375 c          326 g          305 t
ORIGIN      
        1 gaagaagcca ggactcattc tctccgtctg tagaagtcgg tagaaatcgc tctttccaat
       61 tcgatgaatc catagtatgt tgggcgctcc ggcagggact atccaatcac ccgtctacct
      121 gtcatttcca ttttttgttc agcactcaat cattcctggg gggttatgcg cctgatttca
      181 ccacctgaac aaggccagct cgttgatgtc tgcagtcggc gatttgtcgt caccgatgtt
      241 cgacgcacca gtcttccaat tgatccactc ctcaacggcc ttcctcaacc tcaacacctg
      301 attaccctgt cttccgtcga agacgacgct ctgggtgaag aactgcaagt catttgggaa
      361 cttgaaccag gcgcccaggc ttttgaacgc atggaactcc ccgaaccatc cgggtttgac
      421 gaaccagccc ggctggatgc gtttctggat gccgttcgct ggggcgccgc ctccagtgcc
      481 gaaatgcgaa tgctccagtc tccgtttcgg agcggcatcg agattgaaga ttatcaactt
      541 gacccggtgg ttcgggcgat tcaaatgccc agggtgaatt tgttgattgc agatgacgtg
      601 ggcctcggaa aaaccattga ggcgggcctt gtccttcaag aactcgtcat ccgacatcgg
      661 gtccggcggg cgttgattat ctgcccggct tcccttcaga tccagtggca ggaacaaatg
      721 cgcgataagt tcgggcttga atttcgcatc attgacggcg atgccatgaa acggctccgg
      781 cgaacccgtg gcattcacgt caatccctgg tcgcattatc cccgattgat tacctcaatt
      841 gacttcttta agcgcgaaca accgctccgg ctctttcggg caaccctgcc tcacgagggt
      901 gaattgaaat acccccgccg ctacgatctg ttgattgtgg atgaggctca taacgtagcg
      961 ccgtcagcga ccggggccta tgccattgat tcactccgca cggctgccgt tcggctgatt
     1021 gccgaccact ttgaccaccg actgtttttg accgcgacgc ctcataacgg gtattcagag
     1081 tcattcaccg ccttgctgga gttgctcgac ccacagcggt ttgcccgagg catatcgccc
     1141 gaccggcgcc agttgcaagc cgttatggtg cgacggctca aacgtgaact tccccccaga
     1201 tgggatggaa caccccggtt tccagaacgg aaactggaag ccctttctgt cgcctacagt
     1261 gagcaggaac a
//
LOCUS       DAOQDO010002230         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135060_length_1270_cov_2.683951, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002230 DAOQDO010000000
VERSION     DAOQDO010002230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135060_length_1270_cov_2.683951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKZ53_26240"
     CDS             complement(<1..395)
                     /locus_tag="PKZ53_26240"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ44010.1"
                     /translation="MLSLLRATSAGEDPGMRLLRYSLAVIMIIHGLTRITIGGVPLFG
                     AFLSSVGFPFGTAIAGAITLFEIIGTLTLISGYFLIPISLGFIFQLLMGIILVHWKEG
                     WFVVGAGNNGMEFSVLLMSGFASVICSAW"
     gene            954..>1270
                     /locus_tag="PKZ53_26245"
     CDS             954..>1270
                     /locus_tag="PKZ53_26245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44011.1"
                     /translation="MLFGSAPVMAGQTQQSPAPSLFTYVSTGKASPSTITPSDSVVET
                     RLIQINFDLLAESRSQALRFPLGGTEFEAARTDFEVRSETSFTWRGKFPDDGGDITLT
                     VVDG"
BASE COUNT          357 a          310 c          265 g          338 t
ORIGIN      
        1 atccaggccg agcagataac ggacgcaaat ccgctcatca acaacacgct gaattccatt
       61 ccgttattcc cggcaccgac gacaaaccag ccttccttcc agtgaaccag aatgattccc
      121 attagtaact gaaaaataaa tcccagtgaa atcggaatga gaaaatagcc gctgataagt
      181 gtcagtgtac caataatttc aaagagagta attgctccgg caatggctgt cccaaacgga
      241 aatccaacgc tcgaaagaaa ggcgccaaac aagggaactc ccccaatggt aatccgggtt
      301 aacccgtgaa tgatcataat gaccgcaagt gagtatcgaa gcagacgcat cccaggatct
      361 tcgcctgcac tggttgcacg caataaagac agcatggtga cctgtccttt atatatttgg
      421 atacgcactg atttacagaa ggcagtacct tactgagaaa tgtgttgaat cgtcaaatga
      481 tatttcttgg agtgctacgg cttgacatag ctttggaatt tggcggcttt caatcacgtc
      541 gccgcactcc atattttaac caaaaagcta aaacctttca ctaaaaggtt aaagattcaa
      601 attaaattta aaatttaaaa ataatttaat ttctataagt ttattgattt aaatagttta
      661 agcaggtgac gcctgtccat tcatcgccga tttgagcctt tttttacgtg gcacgccggt
      721 tgcacttgag aaggatatct tcattcaaat caatctcaga tatgccgact ttttactcct
      781 gttctgtgcc ctcagtgacc tgcagggcac aggccactcg cgagaagaaa atcctggcag
      841 caaccatttt ttcggaggcc tcgatgtccg tttgtactca catcatccaa aaacctgctc
      901 agtcagtgat gacttgcaaa aatattctgt tcgcaatcgt tgtttggtgc ctgatgcttt
      961 ttggatcagc accagtgatg gctggccaaa cccagcagtc accagcccca tccttattta
     1021 cttatgtttc aactgggaag gcgagcccct cgaccatcac cccatctgac tctgttgtgg
     1081 aaacacgcct gatccagatc aactttgatt tacttgcaga atcccggtcc caggcgttgc
     1141 gatttccact tggcggcact gaatttgaag ctgcccgcac tgattttgaa gtgcgtagtg
     1201 aaaccagttt cacctggcgc gggaaatttc cagatgatgg cggtgacatc accttaaccg
     1261 ttgttgatgg
//
LOCUS       DAOQDO010002231         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135287_length_1269_cov_2.571664, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002231 DAOQDO010000000
VERSION     DAOQDO010002231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135287_length_1269_cov_2.571664"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1059
                     /gene="aac(3)"
                     /locus_tag="PKZ53_26250"
     CDS             232..1059
                     /gene="aac(3)"
                     /locus_tag="PKZ53_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /protein_id="HNJ44012.1"
                     /translation="MTDLEIPTVTKQQIISGLQALGVEAGQTLMLHASVKAIGWILGG
                     PIVVLEALMDVLTPDGTVMMMVSWEGNPYQMAGWPAEQQALCLAECPPFHPATSPADH
                     RTLSILTEYLRTWPGACRSTHPLASFAAVGKKASWLTERHSLHYNHGLDSPLARLCES
                     PGQILLLGAPFSNITLLHHAEHLANIPDKRLDRYRMPILQDGQRVWVEIEEFDTSNGI
                     ANFGNWDYFLDIGQAYVASGKGQSTQIGNAPCYLFNADDLKHFGITWMEQNYKSPAR"
BASE COUNT          291 a          314 c          323 g          341 t
ORIGIN      
        1 aaccctgaac ccgataccct gaaccctaat tggtataagt accttgccat accttttctg
       61 agatattggg tttgataaac gcttaatttt tttccgtgtt ttccgtgttt tccgtggtta
      121 agatatttgg aaattttcag taaagtattt atcaatgatc ggttgattga caaagggaga
      181 gtgtggatcg tttttggtct tacctccaat ctctaaagaa agtaagccag catgactgat
      241 ctggaaattc caactgtaac caaacaacaa attatatcgg ggctgcaagc gctcggtgtt
      301 gaagctggcc agacgctgat gctgcatgct tccgtaaagg ccatcggctg gattttgggc
      361 ggtcccattg tcgtgcttga agcgctcatg gatgtcctga cacccgatgg aacggtcatg
      421 atgatggtca gttgggaagg gaatccctat caaatggccg ggtggccagc cgaacaacaa
      481 gcgctttgtt tggctgaatg tccgccattt catccagcga catcaccggc agatcatcgc
      541 accctgagta tcttgactga gtatcttcgg acctggcccg gtgcctgtcg cagcactcat
      601 cctcttgcct catttgcggc ggttggtaaa aaagcatcct ggctgactga acgtcattcg
      661 ttgcactata accatggttt ggactccccg ctggcccggc tctgtgagtc gcctgggcag
      721 atcctgctgc tcggtgcccc attttcaaac atcacgctgt tgcatcatgc cgaacatctg
      781 gcgaatattc ctgacaagcg ccttgatcgc tatcgcatgc cgattttaca agacggtcaa
      841 cgggtgtggg tcgagattga agagtttgac acctcaaatg ggattgcgaa ctttggcaac
      901 tgggactatt tcctggatat tggtcaggcg tatgtggcat cgggaaaggg gcagtcaacg
      961 caaatcggga acgccccctg ttatctgttc aatgctgatg atttgaaaca ttttggtatc
     1021 acgtggatgg aacagaacta caagtcgcca gcccgttaac tcacgcagtt gcggcataag
     1081 cgccaggata aggaaaaacg tgtcctttct gttggtattg aatgacgttg gtgttgggtc
     1141 tggatcccgt cctggccgtg accgtgggct tcgcaccacg gctattacac gacgaccctg
     1201 cgggcctaaa acccttatcc tggcgcttat gacctctgac cccgagcccc tgatcccaaa
     1261 atggtatta
//
LOCUS       DAOQDO010002232         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135366_length_1269_cov_1.893740, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002232 DAOQDO010000000
VERSION     DAOQDO010002232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135366_length_1269_cov_1.893740"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(429..989)
                     /locus_tag="PKZ53_26255"
     CDS             complement(429..989)
                     /locus_tag="PKZ53_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44013.1"
                     /translation="MLISIPFLLISFGIVSGATSYWVDWQSKIFSSLELIIIGFLLWM
                     IVAMALPGVSAVNKMKNMSTTVRVLKKITHAQDAYFKTHGQCASQVEQLVTSPHFPDG
                     LTELESRIGTVGYQGYRIVSFHTMPPTDSLPATYDIQFAPSIRSGILCSGSVCYFLDQ
                     SGIIRHSGKPTELAQVTSELISRLNE"
BASE COUNT          380 a          261 c          282 g          346 t
ORIGIN      
        1 cggccaggac ccgtcgttga atcggattga gaaaaagtgg tggtttatca aatttctttt
       61 gggaatactt ctgttcgctg tgaggcatgg agttctcctt gtgagtggtg acaagtggca
      121 atacttttgg tcggctggtc actccggtcg gagcttttaa agctaagggg ttcaatccct
      181 tatgatccag gtaattttta ttctgagttg attggtgaca aaaactttgt gggtaaaagg
      241 atttacagtg tgtcttttgg acagttgatg tccaaaaaac agcaacattg atttgaactg
      301 atcagagtcg agggataact gaagttcaaa ccgtcacact ggaatttcgc aggagatatc
      361 aggaataatc gtgaggcaag gcaagaatag agggacttgg ggttactgtc aatcaccaac
      421 gtagtgagct attcattgag cctggaaatc agttcgctgg tgacctgcgc cagttcagtt
      481 ggtttcccag agtgccggat aatccccgat tgatctaaaa agtagcaaac agacccgcta
      541 cataaaatac ccgagcgaat ggatggagca aactggatgt cataagttgc tggaagagaa
      601 tcagttggcg gcattgtgtg aaacgaaaca attcgatacc cttgatagcc aacggttcca
      661 attcgtgatt ccagctcagt caatccatca gggaaatgtg gtgaagtgac aagttgttca
      721 acttgtgaag cgcactgccc atgagtttta aaataggcat cctgggcatg tgtgattttt
      781 ttcaaaaccc tcacggttgt actcatattt ttcatcttat tcacagccga gactcctggt
      841 aaggccatgg ccacaatcat ccacaagaga aacccaataa tgatcaattc cagcgaacta
      901 aaaatttttg attgccagtc cacccaataa ctggtcgcac ctgaaacgat tccaaagcta
      961 atcagaagaa aaggaatgga aatcagcatg catatccgcc ctaactccag ccactctggg
     1021 tcaattggca actttaccaa ataaaaatca atgtttgtaa atccgctgat tattccaaaa
     1081 atgcaaagaa tcaaataact ggttactcca aattttactt ttgaaatcca gggccagtac
     1141 accagtcttc gttttattgt taaagaaatc cccgatgatt tcgtaacata tggattcaat
     1201 ggttgacacc tctgacactg gttcaaggtt cggtcgaaca aatccgactg atgataaatc
     1261 tcacatctg
//
LOCUS       DAOQDO010002233         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135476_length_1268_cov_2.671063, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002233 DAOQDO010000000
VERSION     DAOQDO010002233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135476_length_1268_cov_2.671063"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKZ53_26260"
     CDS             complement(<1..208)
                     /locus_tag="PKZ53_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ44014.1"
                     /translation="MRSLIHTLLRFLAVRQGRCVGMYRRICAPDGTEWAAYLKAQDIL
                     FEIGDNCSIQTNVTITDPKYVRLGN"
     gene            complement(363..>1268)
                     /locus_tag="PKZ53_26265"
     CDS             complement(363..>1268)
                     /locus_tag="PKZ53_26265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44015.1"
                     /translation="LRLFTGYGSVVQVTGILSMVLYSIEKVIAGAFLGVQSTALFDIG
                     EKLPVMTSQIPSSMNMAFVPAMSHLATLEQKAEVIKLYLKGSRYLNTMTGLMMGFLAA
                     FAAPLLTIWIGHNDQFEVAALIMVWFCLPYQMNVLTGPGSGFHRGNNRPVRELLYPVS
                     QAICVVLAVGTGFLIWGKTIQVITIAVAISMVASAVWYMAFTNRTIGITQVEFLRRVL
                     VPGLVPYGFGMLVRIIVSPALHWAGSNRWILLVIISLAGVLYCGLVLGFFYRALCDWN
                     EREFLRRQITHTLGSFFRFNLLKSA"
BASE COUNT          364 a          335 c          297 g          272 t
ORIGIN      
        1 tgtttccgag ccgcacatat ttcggatcgg tgatggtgac attggtctga attgaacagt
       61 tgtctccgat ttcaaacaga atatcctggg cctttaaata ggccgcccac tcggtcccat
      121 ctggtgcaca tattcggcgg tacattccaa cacaacgccc ctgtcgaacc gccaaaaatc
      181 gcaaaagtgt gtgaatcaga cttcgcatac aatttccctt gccggactat ggaagattga
      241 gaaacctttc ctgattgaaa cggtgctgag gacatacaaa atgtcctcgc gaatgttaaa
      301 agaactcatt tgaaatcaat atattaagtg ctttgtccac gcgtggacaa aatcgagaag
      361 tttcaggccg atttcagcaa attaaaccgg aaaaatgacc caagggtatg cgtgatttgg
      421 cggcgcagaa attcgcgttc attccagtca cacaatgccc gataaaagaa cccaaggacc
      481 agaccacaat aaagcacacc tgccagactg atgatgacga gcaatatcca ccgattggat
      541 ccggcccagt gaagcgccgg tgaaacgata atccgaacca gcattccaaa cccatagggc
      601 accaaccccg gcaccagaac ccggcgcaga aattcaacct gggtaattcc aatggtccgg
      661 ttggtaaacg ccatatacca gacggcgctt gccaccatgc tgatggcgac ggcaatggta
      721 atcacctgga tggttttccc ccagatcaga aatccagtgc ccacggccag cacaacgcat
      781 attgcctgcg aaactggata gagcagttcc cgaaccgggc gattgtttcc tcgatgaaac
      841 ccagacccag gcccggtgag cacattcatc tgatagggca ggcaaaacca gaccataatc
      901 aacgcggcaa cttcaaactg gtcattgtgt ccgatccaga tggttaacag cggagcggca
      961 aaggcggcca aaaagcccat catgaggcca gtcatggtgt tcagatagcg cgagcctttt
     1021 agatagagtt tgatgacttc tgctttttgt tcgagggtag ccaggtgtga catggccggg
     1081 acaaacgcca tattcatcga agacggaatc tggctggtca ttacgggtaa cttttcacca
     1141 atatcaaaca gcgctgtgct ttgaaccccg agaaatgctc cagcaatcac tttttcgatg
     1201 ctatagagca ccatgctcag aattccagtt acctgaacca ccgacccata cccggtaaag
     1261 agccggag
//
LOCUS       DAOQDO010002234         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135891_length_1266_cov_3.325351, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002234 DAOQDO010000000
VERSION     DAOQDO010002234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135891_length_1266_cov_3.325351"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PKZ53_26270"
     CDS             <1..1181
                     /locus_tag="PKZ53_26270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44016.1"
                     /translation="LKDSSFSILHSLVGGVMDQHLKKTFQILEEKAATRCDICHQADL
                     FDPITGICQRCEPIATVALAQLAEEQAENFHPEPTPVQTPTPVVTSPLATYHPTLSAR
                     DRWLILREAFGLYRDHSQVFLSIVLRFEAPLAAIFLALALFPDKTHWPLVAILSVIVS
                     FLLPNEAREGSLICAIFDSLPDRVPNAGRAIQFANRRNHQHLWAMWNVKVKQWLGSVV
                     GFLIMGLVGAMLQPVFGPLPLSALAITFGLWFSWLALSHNAFYRQILVLEGRSPEEAQ
                     RRSDWLANRNCGWLSLLNQTRWLGMLLIAFMGLSPISTKVLVSGFLALGGPLALGICA
                     TTTPLWFMVLMVLKILLGPFLTITETLSYLVLRRRAEGMQFQFQYTKEEDDRLKEFMT
                     "
BASE COUNT          293 a          332 c          309 g          332 t
ORIGIN      
        1 acttaaagga ttcttcattc tcaattcttc actcactggt tgggggtgtg atggaccagc
       61 atttgaaaaa gacatttcag attcttgagg aaaaagcggc cacccggtgt gatatttgtc
      121 accaggcgga tctgtttgat ccaataactg gaatctgcca gcggtgcgag ccaattgcca
      181 cagtggccct ggcccagctt gccgaagaac aggctgaaaa tttccatcct gaacctaccc
      241 cagtccaaac cccgacgcca gttgttactt ccccattagc cacctatcac ccaacgctct
      301 cagctcgcga ccgctggctg attttacgcg aagcgtttgg actctatcgt gatcattcac
      361 aggtttttct gagcatcgtg ctcaggtttg aagcaccgct ggcagcaatt tttttggccc
      421 tggctttgtt ccctgacaaa acacactggc cactggtcgc gattctgagc gtcattgttt
      481 cgttcttgct gcccaacgaa gcccgcgaag ggtcactcat ctgcgccatt tttgatagtc
      541 tgccagaccg cgttccaaat gccgggcggg caattcaatt tgccaatcgt cgcaaccacc
      601 aacatttgtg ggctatgtgg aacgtcaaag taaaacaatg gttgggttca gtggtcggct
      661 ttttgatcat ggggcttgtc ggagcgatgc tgcagccagt ttttgggcca ctcccgctct
      721 cagcactcgc catcacgttt gggctctggt tctcctggct ggcgctgtca cacaatgcct
      781 tttatcggca aattcttgtg ctcgaaggtc gttcacctga agaagcccag cggcggagcg
      841 actggctggc aaatcgtaat tgcggatggc tgtcgttgct caatcaaacg aggtggctgg
      901 gaatgctcct gatcgccttt atggggttgt cacccatttc aacaaaggta ctggtgagtg
      961 gctttctggc acttggtggc ccactggcgc ttggaatttg tgccacaacc actcctttgt
     1021 ggtttatggt gctgatggta ttgaaaatcc tgcttggccc ttttttgacg attaccgaaa
     1081 ccctgtcgta tctggtgctt cggcggcggg cagaaggaat gcagttccag ttccaataca
     1141 caaaagaaga agatgatcgg ctcaaagaat tcatgactta agtaccttac cgaaaacttc
     1201 cagacatttt gaaccacgga aaacacggaa aacacggaaa aaaatcaacc acttaccaaa
     1261 cccaat
//
LOCUS       DAOQDO010002235         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135933_length_1266_cov_2.592073, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002235 DAOQDO010000000
VERSION     DAOQDO010002235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135933_length_1266_cov_2.592073"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKZ53_26275"
     CDS             complement(<1..408)
                     /locus_tag="PKZ53_26275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44017.1"
                     /translation="MYHHLNPEAFDAHPAHSTRRRITSSSDLRAWLDQTGPNPGRDQT
                     TTVTFIIDTDGWLWIADRHSEHIACAAGRDVLSAGEMTFDGSQTEIFVSDVSNQSLGY
                     CPEPDSWPAVDSALNRAGIPHPSGFTAEFVFRLC"
     gene            complement(377..>1266)
                     /locus_tag="PKZ53_26280"
     CDS             complement(377..>1266)
                     /locus_tag="PKZ53_26280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ44018.1"
                     /translation="PAHIWIYSVLTTVGLGWLDYVTGYEISFSFFYLIPVFLVTWYIS
                     RRSGLIMALLTIVVWVLSNRLAGQQYSSEVIRIWNAILRLAFFALTVNLLQIVKATLD
                     WERQLSRIDYLTGAVNSREFHQILQTEIIRSQRYHHPISLAYLDLDNFKTINDQFGHQ
                     AGDKVLQFISSTIRNSLRKVDTLARLGGDEFAILLPETNHTAAQGALLKVREAILSQI
                     PPQYAAVTVSIGVVSFEHPPDSPEAMLHHADNLMYQVKSSGKNHVLFAEPDHEEQHQL
                     TQQNHFLNERVSPSESRGI"
BASE COUNT          320 a          295 c          348 g          303 t
ORIGIN      
        1 gcataaacga aagacaaatt cggctgtaaa accggatggg tgcgggattc cagcccgatt
       61 gagtgcgcta tccactgctg gccaggaatc aggttccggg caatatccga gcgattggtt
      121 gctgacgtct gaaacaaaga tttccgtttg tgaaccgtcg aaggtcattt ccccggctga
      181 aagcacatcc cgcccggcgg cacaggcaat gtgttcagag tgacggtctg caatccaaag
      241 ccatccgtca gtgtcaataa taaaggtaac cgttgtggtt tgatcgcgac ctgggtttgg
      301 cccggtttgg tcaagccagg ctcgcaggtc tgaactcgaa gtaatccgtc tccgcgtcga
      361 atgcgccgga tgggcatcaa atgcctctgg attcagatgg tgatacacgt tcattcagaa
      421 aatgattttg ttgagtaagc tggtgctgct cttcgtgatc tggttcagca aataaaacgt
      481 gatttttgcc actggatttg acctgataca tcaaattgtc ggcgtggtgg agcatggctt
      541 cgggggagtc gggcggatgc tcaaatgaaa ccactccgat actgacggtg acggctgcat
      601 attggggtgg aatctgactg aggattgctt cacggacttt gagcagtgct ccctgggctg
      661 ccgtgtggtt ggtctccggg agcaagatgg caaattcatc tccgccaagt cgtgccagcg
      721 tgtccacttt ccgcaacgag ttgcgaattg tgctcgaaat aaactgcagc actttgtctc
      781 cggcttgatg gccaaactgg tcattgattg ttttgaagtt gtccaggtcc aggtacgcca
      841 gtgaaatcgg atgatgatag cgttgtgaac ggatgatttc agtctggaga atctggtgaa
      901 attcacgcga gttgaccgcc ccggtcaaat aatcaatccg tgaaagctgg cgttcccagt
      961 caagggttgc tttgacaatt tgcagcaggt tcaccgtcaa tgcaaaaaag gccagtcgga
     1021 gaatcgcatt ccagattcgg ataacttcgg atgaatactg ttgacccgcc agccggttcg
     1081 aaagaaccca aacgacaatg gtgaggagtg ccattatcaa acctgaccgc cggctgatat
     1141 accaggtgac aagaaagact ggtatcaaat aaaagaatga aaacgagatt tcgtaaccag
     1201 tgacatagtc aagccagcct aaccccacgg tggtcagtac cgaatatatc caaatgtgcg
     1261 ccgggc
//
LOCUS       DAOQDO010002236         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_135976_length_1266_cov_2.180842, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002236 DAOQDO010000000
VERSION     DAOQDO010002236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135976_length_1266_cov_2.180842"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..277)
                     /locus_tag="PKZ53_26285"
     CDS             complement(41..277)
                     /locus_tag="PKZ53_26285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44019.1"
                     /translation="MDGTPLQVLPGGGIRIELNPPTVQHFLATVRTESLRAMVAGILS
                     FAIILAFVLILVKSLLGERQARRRLAQAYQQIYR"
     gene            complement(637..>1266)
                     /locus_tag="PKZ53_26290"
     CDS             complement(637..>1266)
                     /locus_tag="PKZ53_26290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ44020.1"
                     /translation="PTAKLVGLAVDLSTAEGVQTCVSKHPEIDILVNNLGIFEPKPFL
                     EIPDADWQRFFEVNVLSGVRLSRAYLPGMLARNWGRIIFISSESGVNIPAEMIHYGMT
                     KTAQLAVARGLAETTAGTGVTVNSVLPGPTRSEGVEEFLQSMTLHQGISLETLEKQFF
                     ETVRSSSLLKRFATVEEVANLVAYVASELSSATNGAALRVDGGCVRSVV"
BASE COUNT          316 a          343 c          316 g          291 t
ORIGIN      
        1 ggcattcatc cccgcgatca atcgcggggt tttctgccgg ttatctataa atctgctgat
       61 atgcctgagc cagccgtcgg cgggcctggc gttccccaag cagagatttt actaaaatca
      121 aaacgaacgc caaaataatg gcaaatgaaa gaatacccgc aaccatcgcc cggagtgatt
      181 cggtgcgcac cgtagccaga aaatgttgaa ctgttggtgg attcagttca atccgaattc
      241 cgccgcctgg taaaacctgg agaggcgttc cgtccacctg gatttgactg atcagtgccg
      301 catctgagtt tgaccagagg ttgatgaccg gaagcgatac ttgaaacaga tatagttacc
      361 aaaaatagaa tcaaatctga cagaccttcg tcccaagacc aagaattgga cctgaaggtc
      421 gtgtctgact gactgaacga tacccctggt gttggcgcgt ggtttcccgc ctcaccgtga
      481 ccaggagggg aaaagtcagg cgttgctgcc ttctaagggc agcaatgcct gactttcgct
      541 tcaatttcca aatacagaat tgttggtgct cgtatcggga aagagttaag tagttcgatt
      601 caatgagttt agctgactac tgactgcaaa ctggccttaa acaacactgc gcacacagcc
      661 accatccacc cgcagggcgg caccattggt ggcagacgaa agttcactcg ccacgtaggc
      721 caccaggttg gcgacttctt caaccgtggc aaagcgtttc agaagcgatg acgaccgaac
      781 cgtttcaaaa aattgctttt caagcgtttc cagcgagata ccttgatgga gtgtcatact
      841 ttgcaggaat tcctccaccc cttctgaccg ggttggtcct ggcagcaccg aattgacggt
      901 gacaccggtt cctgctgtcg tttcggccag cccacgggca accgccagtt gtgccgtttt
      961 ggtcattcca taatggatca tctcggcagg gatgttgacg cccgactcac ttgaaataaa
     1021 gatgatccgt ccccagttcc gggcgagcat tcccggcagg taggcccgac tcaaccgcac
     1081 accactcaac acattgacct caaaaaagcg ctgccagtca gcatctggga tttccaggaa
     1141 gggtttgggt tcaaaaatcc ccaggttgtt gaccagaata tcaatttccg ggtgtttcga
     1201 tacacatgtc tggacacctt cagccgtgga cagatccacc gccagtccaa ccagtttggc
     1261 cgtcgg
//
LOCUS       DAOQDO010002237         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_136105_length_1265_cov_3.667769, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002237 DAOQDO010000000
VERSION     DAOQDO010002237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136105_length_1265_cov_3.667769"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>1265
                     /locus_tag="PKZ53_26295"
     CDS             140..>1265
                     /locus_tag="PKZ53_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44021.1"
                     /translation="MLPAHCFLTPAAVVEGQVDLTQLRAIVTMNLKQALRPGKDPVTG
                     SVGNPIRQIVLSMSAFGALFAFNADRFADLPSYLIMLFASTFLIASLNIAPDPYDVRQ
                     RNLETFHSKAITQKTLLMARIVVLFIIVNFIAISYALIPLAVSVFYLDCPVVLAVCAL
                     ILLLIGCFSLVVYWLVVLMVAAQFIGLDRVRQMSQGLFLLVLLGIIGISFDFFGFNQH
                     PFSQSPEQVSLIDSAWVKFLPSTWFVDALVGDLSLVANIERTVMLATVCGAIGILWRL
                     DTDGYFPRLVEKLSEPASQPTSRPLSVKLLNLYRLIPPVRRWVIPDHTCALASLVLTV
                     TQREEISRIRTLVPRVFMILFFLAGLSSFRPEARFFGLLML"
BASE COUNT          249 a          320 c          298 g          398 t
ORIGIN      
        1 aggatggata cccgtcaagc agcccttaag atggtgattc ccgtcctctg tcattttatc
       61 tgtgtttgga gcacggactt ggtaagatga ccgggttcca ttcttctgtc atccaaatta
      121 caacttcgaa acccaaacta tgctcccagc ccattgtttt ctcacccctg ccgccgtggt
      181 cgaaggtcag gttgatctga cacaacttcg ggccatcgtc accatgaatt tgaagcaggc
      241 actcagacct ggaaaagacc cggttacagg aagcgttgga aacccaatcc gtcagattgt
      301 gctttcaatg agtgcatttg gtgccttgtt tgccttcaat gccgaccggt ttgctgactt
      361 accgagctat ctcatcatgc tctttgcctc aaccttcctg attgcgagcc tcaatattgc
      421 cccagacccc tatgatgttc gccagcgtaa tttggaaaca tttcattcaa aagcaattac
      481 ccaaaaaaca ctgctcatgg cccgcattgt ggtgttattt atcatcgtca attttattgc
      541 gatcagttat gccttaattc cattggcggt ttcggtgttt tatcttgact gtccggtcgt
      601 actggcggtg tgcgcgttga ttttactgtt gattgggtgt ttttcactgg ttgtgtactg
      661 gctggttgtc ctgatggtgg cagcccagtt tatcggtctt gatcgggtcc gccagatgtc
      721 tcaaggactc tttctcctgg tactgctcgg tattattggc atctcatttg atttttttgg
      781 gttcaatcag cacccctttt cccaatcgcc tgaacaggta tcgttgattg attcggcctg
      841 ggtaaaattt ttgccatcaa cgtggtttgt ggatgcactg gttggtgatc tctcactggt
      901 ggccaatatc gaacgaaccg tcatgctggc caccgtctgt ggtgcgattg gaattctctg
      961 gcgacttgat actgacgggt attttccgcg cctggttgaa aaactctctg agccagcttc
     1021 tcaacccaca tccagaccgc tgtcggtaaa attactgaac ctgtaccgtc tgatcccgcc
     1081 agttcgccga tgggtgattc ccgatcatac ctgtgcgctg gcatcgctgg tcttgactgt
     1141 cactcagcgt gaagaaattt cacgcattcg gaccctggtc ccgcgagtgt ttatgattct
     1201 atttttcctg gctggactga gttcttttcg tccagaagcc cggtttttcg ggttactgat
     1261 gttga
//
LOCUS       DAOQDO010002238         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_136344_length_1264_cov_3.224979, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002238 DAOQDO010000000
VERSION     DAOQDO010002238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136344_length_1264_cov_3.224979"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..163
                     /locus_tag="PKZ53_26300"
     CDS             8..163
                     /locus_tag="PKZ53_26300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ44022.1"
                     /translation="MAASRGYTDVVKELLEAGANPDIQFGGNTALQLAQERNRDAVVK
                     ILTEKRK"
     gene            complement(271..912)
                     /locus_tag="PKZ53_26305"
     CDS             complement(271..912)
                     /locus_tag="PKZ53_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44023.1"
                     /translation="MLIHAQSPPRRCEICHQTDCFDPTTNHCARCSGVVASLLIPTEP
                     VKPSKATHRQWRITETALAVFILLTIGSIAIPNLMAAKRRSHSEHHALNMIRQIHRAQ
                     ESYQKAKGQGNYTPDLEALGKLEIDGKPVLSPDVVNAQKQKVHGYQLGPIIVQPRISG
                     RPARYHITAFPCYESELSRCGSDCFYIDQTGIVRHSGKPNVIPTAWSEPLLDE"
BASE COUNT          292 a          308 c          313 g          351 t
ORIGIN      
        1 cctgcacatt gctgcttcgc gagggtatac agacgtcgtc aaagaactcc ttgaggcggg
       61 cgccaaccct gacattcaat tcggtggtaa caccgccctg caactggctc aggagcgcaa
      121 ccgcgatgca gtggtgaaaa tactgactga aaaaagaaaa tagaatgaag aaagtggttc
      181 gtggttcgtg gttagtacct ggtacttagt gctttggaac caatgccttc caagctccaa
      241 gctccaaaaa cgaaacaact tcctggttct tcattcatcc aaaagtggct cactccaggc
      301 agttgggatc acgtttggct ttcctgaatg ccgtacaatg ccggtctggt caatataaaa
      361 acagtcgctg ccacatcggc ttagttctga ttcgtagcaa ggaaacgctg tgatgtggta
      421 tctggctggc cggcctgaga ttcgaggttg gacaatgata ggcccaagct gatagccgtg
      481 aaccttttgt ttctgagcgt tcacgacgtc aggggacaac accggttttc catcaatttc
      541 cagtttgcca agcgcttcca ggtcaggggt gtaatttccc tgaccttttg ctttctggta
      601 ggactcctgc gctcgatgaa tctggcgaat catgttcaag gcatggtgct cagagtgact
      661 ccgccttttg gccgccatca ggtttgggat ggcaattgac cctattgtca gcaaaataaa
      721 aaccgcaagc gccgtctcag tgatacgcca ttgccgatga gtggccttcg aaggtttgac
      781 aggttccgtc ggtatcaaaa gcgatgccac cacgcccgaa catcgggcgc agtggttcgt
      841 cgttggatca aaacaatctg tttgatgaca gatttcacac cgtcgaggtg gtgattgggc
      901 gtgaatcaac ataatccctc aatttcaaac cctcttttat tctcaattga tcagggaaca
      961 tccgtttttg atgcactatg ggcttggaag tttaagagcc atgtctgatt tgattcaagg
     1021 tgttcctgga atcgaatttt tggtattgtg caggcattac cgtgatttct cgcactattg
     1081 cttcctcaag gtcttttgta tgcgaaactt ttgttcggtg ctcttgtgtt gtgggccatt
     1141 gaactcagga atattgtcct gttcgttcgt tctggctctg atattgagta ctatttccgc
     1201 caatggtcat ccgtggccaa ccgacgctcc ccagaaggtt gagcctgccc aatccacttc
     1261 accc
//
LOCUS       DAOQDO010002239         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_136788_length_1262_cov_3.018227, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002239 DAOQDO010000000
VERSION     DAOQDO010002239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136788_length_1262_cov_3.018227"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PKZ53_26310"
     CDS             complement(<1..465)
                     /locus_tag="PKZ53_26310"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ44024.1"
                     /translation="MRSDLSNVGMNLSQSEKVNSLKVLWFTQPFAQYPSPPMALPCER
                     AVMDEPAFEVFYRKTAPRLWAYVHRASGSSTVADDVLQEAYFRFLRSPGTAMDEAQMK
                     SYLFKIATNLLNDHWRRQKREHPGLPAHHSEPVAPGDFSQHDDVTQALNQLNH"
     gene            complement(462..1022)
                     /locus_tag="PKZ53_26315"
     CDS             complement(462..1022)
                     /locus_tag="PKZ53_26315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44025.1"
                     /translation="MSFIGDGFVLGEVSQLLIVQTPLPAGLDELVPIIVIPSLCLMIA
                     WLAWVLNRTTYLKAKAQIELHNQLVQRIHSTQDVLRLLETEAGRQLLDALSLERPTTR
                     EEIVNSVRKGIILSMVGLGGLLLRLIFPMGYGVFILASILVGMIGAGLLVSSFVVYRL
                     SQSWGLISETRPKNREMSPHDTSLFR"
BASE COUNT          333 a          327 c          292 g          310 t
ORIGIN      
        1 atggttcaac tgattcagcg cctgtgtcac atcatcgtgt tgagagaagt ctccaggcgc
       61 aacgggttcg gaatgatggg ccggcaatcc tgggtgttcc cgcttttgac ggcgccaatg
      121 atcgttcaag agattggttg caattttgaa aaggtaggat ttcatctgag cttcatccat
      181 tgcagttcct ggcgaacgaa gaaatcggaa atatgcctct tgcaaaacgt catcagcgac
      241 cgtcgaactt cccgaggctc gatgcacata agcccacaat cgcggcgctg tctttctata
      301 gaaaacctcg aaggcgggct cgtccatgac agctctttca caaggtaatg ccattggggg
      361 cgaagggtat tgggcgaagg gctgagtgaa ccacaatact ttcaatgagt tcactttctc
      421 agattgcgac aggttcattc caacattgga taaatccgat ctcaccggaa aagactcgta
      481 tcgtgcgggc tcatctctcg atttttcggt cgggtttctg aaatcaaccc ccaggattgt
      541 gataatcggt agacaacaaa tgatgaaaca agcaggccag ctccgatcat tccaaccaaa
      601 atactggcca ggatgaaaac gccatacccc attggaaata tcaggcgcag caacagtccg
      661 cccaatccca ccatactcag aatgatccct ttccgcactg aattcacaat ttcttctcgt
      721 gtggttggcc gttccaggga aagagcgtcc agtaattgcc gaccagcttc ggtttccaaa
      781 aggcgcaaaa catcctgggt cgagtgaatt cgctgtacca gttggttatg caactcaatc
      841 tgggcctttg ctttcagata ggtcgttcgg ttcaacaccc aggccagcca ggcaatcatc
      901 aaacacagac tgggaatcac aataattgga accaactcgt ctaaaccagc cgggagcgga
      961 gtttgaacaa tcagcaattg agatacctct cccaacacaa aaccatcgcc aatgaaactc
     1021 atagttctct cctgaattca acttcacctt tgattggggc tgaagaactg gttttctttc
     1081 atctctcatc cttcagccct cagccctcag cccgatggtt tttcagctcg ttttcttcag
     1141 cccaaaggtt gaaatggctt ccagtgccgg atcccgatgt gcgcgataca actcaaatga
     1201 tggtggagta tagagtacag gtgcaatcca ggtgcggtaa tcaaatggcc aggaactttg
     1261 cc
//
LOCUS       DAOQDO010002240         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_137002_length_1261_cov_3.932836, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002240 DAOQDO010000000
VERSION     DAOQDO010002240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137002_length_1261_cov_3.932836"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..621
                     /locus_tag="PKZ53_26320"
     CDS             115..621
                     /locus_tag="PKZ53_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNJ44026.1"
                     /translation="MAFDAFLKMEGIQGESRDAKHQNEIEVLAYSWGVSQMNSNSSSS
                     HGSKSAERANFSEFNFTKALDKATPHLAFACASGQHFSKATIEICRAGGTSKQTFMRY
                     TLQDVMITNYSVNGVGGGGDGESIPTENIQLSFGKIEWNYTPTNQTTGQAMGNISKGW
                     DLTQNKAA"
     gene            complement(717..1151)
                     /locus_tag="PKZ53_26325"
     CDS             complement(717..1151)
                     /locus_tag="PKZ53_26325"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ44027.1"
                     /translation="MTLSVLEFGSLVAGVDYVLRPGGYAVVRNSAGQIALISAPAGYV
                     LPGGGQEPYESPEAAAIRESYEECGLNIHLQSLIGTVDELVFAKAEGIYFRKRCTFYR
                     AVLIQQDDCGESDHHLIWVTPAQAGELLRFESQRWAVTTCGP"
     gene            complement(1163..>1261)
                     /locus_tag="PKZ53_26330"
     CDS             complement(1163..>1261)
                     /locus_tag="PKZ53_26330"
                     /inference="COORDINATES: protein motif:HMM:NF023134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNJ44028.1"
                     /translation="DTSRNSRRQWCDMQTCGNLAKVRRFRSRQRTS"
BASE COUNT          350 a          326 c          300 g          285 t
ORIGIN      
        1 attgatgccg gcaggatgcc ggcgctccca ggaaatattt atctgaaatg ctataagcac
       61 caggaatcca cccaaactta ctcacttaac cacacttaaa ggagatctat tgtcatggca
      121 tttgatgcat ttttgaaaat ggaaggtatc cagggtgaaa gccgggatgc caaacaccaa
      181 aacgaaattg aggtcctggc ctatagttgg ggggtgagcc agatgaattc taattcgtcc
      241 agttcacatg gttccaaatc agccgaacgg gcaaacttca gtgagtttaa ctttacgaaa
      301 gctctggata aagcaacccc acacctggcg tttgcctgtg ccagtgggca acacttttct
      361 aaagccacaa tcgaaatctg ccgggctggc ggaaccagca aacagacctt tatgcggtac
      421 acgttgcaag acgtgatgat cacgaattac tcagtcaacg gcgttggtgg gggtggggat
      481 ggggaatcaa tcccaactga aaacatccag ttgagttttg gcaaaattga atggaactat
      541 acaccaacca accagaccac cggtcaggca atggggaaca tttccaaggg ttgggatttg
      601 acccagaata aagccgcctg acgatcacaa ctggttgaat cattacaaat gaaacaggta
      661 catcggagtt tgtacctttg aaagtcaatt caaagggaag actccgatca acttttttat
      721 ggaccgcatg tggtcactgc ccaccgctga ctttcaaacc gcaacaattc accggcctga
      781 gcaggtgtca cccagatcag gtgatgatca ctttccccac aatcatcctg ctggatgagc
      841 accgccctat aaaaagtaca ccgcttgcgg aagtaaattc cttcagcttt ggcaaaaacc
      901 agttcatcaa ccgtgccaat caaggattgc aagtggatgt tcaacccgca ctcttcataa
      961 ctttcccgga tggccgcggc ttcaggtgat tcatatggct cctgaccacc accaggcagc
     1021 acatatcctg ccggtgctga gatcagggca atctgtcccg ccgaatttcg gaccacggca
     1081 tatccgccgg gtctcagcac gtaatcaaca cctgcgacca gagagccaaa ttcaagcact
     1141 gaaagtgtca tacagccgtt ttctaggaag ttcgttgtcg cgagcgaaac cgtcggactt
     1201 tggccaggtt gccacaggtc tgcatatcgc accactggcg gcgactgttg cggctggtat
     1261 c
//
LOCUS       DAOQDO010002241         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_137044_length_1261_cov_2.655058, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002241 DAOQDO010000000
VERSION     DAOQDO010002241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137044_length_1261_cov_2.655058"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1261
                     /locus_tag="PKZ53_26335"
     CDS             <1..>1261
                     /locus_tag="PKZ53_26335"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNJ44029.1"
                     /translation="FGEEFETAYAKAEEEKLYNKQVRARDLYGRMMRTLAQTGNGWMT
                     FKDKSNKACNQTAKPGAVVHLSNLCTEILEITSASETAVCNLGSVNLARHVVDGKFDF
                     EKLATTVRLAVKQLDRVIDINFYPIRSTAESNARWRPVGLGVMGLHDVFFQLKLAFDA
                     PEARALSRKIQEEIYFTALSTSCDLATKLGAHKAFPETRAAKGELQFDAWGIVPENTE
                     RWDTLRERIKKHGLRNSLLLAIAPTATIASIAGCYECIEPQVSNLFKRETLSGDFLQI
                     NKYLVNDLKDLGMWTEAMRSRVKMSEGSIQDIEEIPANIREIYRTAWEIPMRSLIDMA
                     ADRGPFIDQSQSLNLFVESPTIGKVSSMYFYAWQKGLKTTYYLRSRPATRIAKTTVSA
                     TTETVAPQPVSESKAIACSLENPESCEA"
BASE COUNT          329 a          352 c          319 g          261 t
ORIGIN      
        1 tttggcgaag aatttgaaac cgcttatgcc aaagccgaag aagaaaagct ctataacaag
       61 caagtccggg cccgtgatct ctatggtcga atgatgcgca cgctggccca gaccggaaac
      121 ggctggatga cctttaaaga caagtccaac aaagcctgca accagaccgc caaacccggc
      181 gccgtggtgc atctgtcaaa cctctgtact gaaatccttg aaatcacttc ggccagcgaa
      241 actgctgtct gcaatctagg ctcagtcaac ctggcccggc acgtggtgga tggcaaattt
      301 gattttgaaa aactggccac gaccgttcgg ctggctgtca aacagcttga tcgggtgatt
      361 gacatcaatt tctatccgat tcgaagcaca gccgaatcca acgcccgctg gcgtccggtt
      421 ggactgggcg tgatgggact gcacgacgtg ttcttccagc tcaaactggc gtttgatgca
      481 cccgaagccc gtgccctgtc gcgcaaaatt caggaagaaa tttacttcac cgcgctttca
      541 acctcgtgcg atctggcgac caaactcgga gcgcacaagg catttcctga aactcgcgca
      601 gccaaaggtg aactgcaatt tgatgcctgg ggtattgttc cggaaaacac cgaacgctgg
      661 gacacactcc gcgagcgaat caaaaagcac ggcttgcgca actcgctgct gctggcgatt
      721 gctccaacag ccacaattgc ttcaattgct ggatgttatg agtgtatcga accgcaagtt
      781 tcaaacctgt tcaaacgtga aacgctctct ggtgacttcc tgcaaatcaa caaatacctc
      841 gtcaatgacc tgaaagatct gggtatgtgg actgaagcca tgcgctccag agtcaaaatg
      901 agcgaaggtt cgattcagga cattgaggaa atcccggcca acatccgcga aatttatcgc
      961 acggcctggg aaatcccaat gcggtcgcta attgatatgg cggctgaccg aggaccgttc
     1021 attgaccaga gccagtcgct caacctgttt gtcgaaagcc cgaccatcgg caaggtctcg
     1081 tcaatgtatt tctatgcgtg gcaaaaaggt ctgaagacga cctactacct gcggtcacgc
     1141 ccggcaaccc gcatcgccaa gacgacggtt tcagcgacaa ccgaaaccgt ggcaccgcaa
     1201 ccggtcagcg aatcaaaggc cattgcctgc tcgcttgaga acccggaatc gtgcgaagcg
     1261 t
//
LOCUS       DAOQDO010002242         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_137237_length_1260_cov_2.821577, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002242 DAOQDO010000000
VERSION     DAOQDO010002242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137237_length_1260_cov_2.821577"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="rlmN"
                     /locus_tag="PKZ53_26340"
     CDS             complement(<1..1038)
                     /gene="rlmN"
                     /locus_tag="PKZ53_26340"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNJ44030.1"
                     /translation="MASVVSRDETLSQTARPSLIGLDRTELATTLATYGEPAFRAKQV
                     FRALYKRMAGSIEDIHELPKSLREKLSPHFTIDTVTLAQTFNSSDGTRRYLFNVNGNE
                     AIEAVWIPEARRSTICLSSQAGCPLACDFCLTAQIGLKRNLTVGEIVGQVVYVLRSEL
                     ERLRREHPTQEVKLPPINLVFMGMGEPFLNYANVMQAIRLMGDADGLEIRPSHVTVST
                     AGIVPRIVDFGKEPNRPRLAISLSAPTDELRNQLMPINRRYPLADLMQACEAFPLAPG
                     EKMTFEYVMLDGINDHDEHAHQILKLTTRLRARNAVKLNLIPHNPAPELPYHPSTEAR
                     VLAFQSILKQKD"
BASE COUNT          297 a          296 c          361 g          306 t
ORIGIN      
        1 atctttttgt ttcagaatgg attgaaaggc caggacacgg gcttcggttg aggggtgata
       61 gggaagctcg ggtgccggat tatgtggaat caggttgagt ttgacagcat tacgtgctcg
      121 gagccgggtt gtcagtttca aaatctgatg ggcatgttcg tcgtgatcat tgattccatc
      181 aagcatcacg tattcaaagg tcattttttc acccggagcg agtggaaacg cttcgcacgc
      241 ctgcatcagg tcagccagcg ggtagcggcg attgatcggc atcaactgat ttcgaagctc
      301 atcggttggg gctgagagcg agatggccag tcgcgggcgg ttgggttctt tgccaaaatc
      361 aacaattcgc ggaacaatgc cagcggtcga aacggtgacg tggctggggc gaatttcaag
      421 tccgtcagcg tctcccatca gccggatggc ctgcatgacg ttcgcataat ttaaaaacgg
      481 ttcacccatg cccataaaca ccagattgat tggcgggagc tttacttcct gggttgggtg
      541 ttctcgccgg agtcgttcca attcgcttcg caggacatac acgacctggc cgacgatttc
      601 gccaacggtt aaattgcgtt tgagaccaat ttgagctgtg agacaaaaat cacaggcaag
      661 cggacagccg gcctgcgatg aaaggcagat cgttgaccgg cgggcttcgg ggatccagac
      721 agcttcaatg gcttcgtttc cattgacgtt gaagagatag cgccgcgtgc cgtccgatga
      781 gttaaaggtt tgcgccagcg tcacggtatc aatggtgaaa tgtggagaca gtttttcgcg
      841 caacgacttc ggaagttcgt gaatgtcttc aattgaaccc gccatccgct tatacagtgc
      901 ccgaaaaact tgtttggctc gaaaggcagg ttcgccatag gttgccagag ttgttgcgag
      961 ttcggtgcgg tccaggccaa tcagcgaagg tcgggcagtt tgggataggg tttcgtcgcg
     1021 ggaaacaaca gatgccacag ggaagtctcc ttacattttt gcggttcagg ttaatcaacc
     1081 attgtgagag gattgatttt gaacgacaag cctttcaggt ggaatagatt taaaagcggc
     1141 gtcaagccgc cgcactccaa aaaattatga cttacatcaa acgtgttcaa acccctgccg
     1201 acgtcgcaca ggcgcgcctt ttatttgaag aatatggcaa cacccgcccc ggtgaccctg
//
LOCUS       DAOQDO010002243         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_137443_length_1259_cov_2.555648, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002243 DAOQDO010000000
VERSION     DAOQDO010002243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137443_length_1259_cov_2.555648"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKZ53_26345"
     CDS             <1..247
                     /locus_tag="PKZ53_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44031.1"
                     /translation="EASRFFETGSLGYSVTNEANRLDGLRLQAREWQVEPLDIEQVYS
                     SYFADKSKFPTGSAEFDHALIMRNIAHEWHTASDLHI"
     gene            complement(349..744)
                     /locus_tag="PKZ53_26350"
     CDS             complement(349..744)
                     /locus_tag="PKZ53_26350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44032.1"
                     /translation="MDRNGPLIVCLSIVLIFLGVFRFAEYRWEQDAPQRQAIARIHQE
                     LLPGMSFKEASAIVNKYKTPELTVGHEPNLLSVAAPFEFGSSNWELWIIFENGYVCAV
                     RIRTEDGPIPGNAPPDKELVEPNPSQTAR"
     gene            complement(903..>1259)
                     /locus_tag="PKZ53_26355"
     CDS             complement(903..>1259)
                     /locus_tag="PKZ53_26355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44033.1"
                     /translation="YRAISLQELEMIEASGFTRFPPRLPDQPVLCLSLHFAHAEQLAR
                     DWIARFCVPPAGFVTQCDVALEFLKNYRTEPVDGTAFREYQIPSDDVSKFNNSIVGQI
                     RIAAEFYGEFQEVDTD"
BASE COUNT          359 a          302 c          292 g          306 t
ORIGIN      
        1 agaagcttcg agattctttg aaaccggttc cctgggatac tctgtcacca atgaggcaaa
       61 ccgcctcgat gggctccgac tacaggccag agagtggcaa gttgagccgt tagacattga
      121 acaggtttat tcaagttact ttgcagataa gtcgaaattc ccaactggaa gtgctgaatt
      181 tgaccacgcg ctgatcatga gaaatatcgc tcacgagtgg catacagcct cagatttgca
      241 tatctgacac agtgtcaacc aggaaatacc caaatacgca gagccggctg attcgccatc
      301 ggttgccaac accttcacca gcccctggct ctgcttatct ccactatctc aacgcgctgt
      361 ttgtgatgga ttgggctcaa ctaattcttt atctggtggc gcgttcccag ggatgggacc
      421 atcttcggtc cgaattcgaa cggcacaaac atagccattt tcaaaaataa tccataattc
      481 ccagttcgaa gaaccaaatt caaagggagc cgcgacactt aacaaatttg gctcatgccc
      541 aactgtaagt tcaggagttt tgtatttatt gacaatggct gaagcctcct taaaagacat
      601 gccaggcaat agttcctggt ggatgcgggc gattgcctgt cgctgaggcg catcttgttc
      661 ccatctgtat tcggcaaatc gaaaaacccc caaaaaaatc aaaacaatac tgaggcacac
      721 aatcaacggg ccgttgcgat ccatccaatc ccttaaacgt gaagattcac ctgggtgagg
      781 agggaaaaag taaggattga gcttcactgg tggaggcaat ggattcatag tttcacttct
      841 aaaatatgcg gaaagcattt taaccttttg gttaaagctt gcaaatcatt gagaaaaaac
      901 tgttaatcgg tgtctacttc ctgaaactca ccatagaact cagccgcaat tcgaatctgg
      961 cccacgattg aattgttaaa ctttgataca tcgtccgaag gaatctggta ttcgcgaaaa
     1021 gcggtgccgt caaccggttc ggtccggtag tttttgagaa actcaagcgc gacgtcgcat
     1081 tgcgtcacaa accctgctgg aggaacacaa aatcgggcaa tccagtcacg agcaagttgt
     1141 tcggcatggg caaagtgcag cgacaaacac aacaccggtt gatcaggtag ccggggtgga
     1201 aatcgggtaa acccactggc ttcaatcatt tcgagttctt gtaaactgat ggcgcgata
//
LOCUS       DAOQDO010002244         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_137669_length_1258_cov_2.682461, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002244 DAOQDO010000000
VERSION     DAOQDO010002244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137669_length_1258_cov_2.682461"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PKZ53_26360"
     CDS             complement(<1..1243)
                     /locus_tag="PKZ53_26360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44034.1"
                     /translation="MMLTIAMIQTILTIVSWKVVPSGWRQPGSAFLAALGFGLSLSLL
                     PVVIGSEYATSVVMLLKSSFFQYLAPGLVVKLMASGSLSWVIGLCLNLAGLLGVTALT
                     IWMANRNQWLRRFRMDAGASTQKARAANWPYPIKVVIRRPQQLIQALLPALAFVPLFL
                     LPELRNLFIKAPVAWGVLSYLIGLFALVSSVPRMITNEGFSVWRWYTYPRSIMGNLFK
                     GSLGWLILAMVVSGVPYVVGLVVRGKFVQPDVFPLLTILVGLPLVSVVCLSAAVLGTD
                     PPTDEKPARLKPVVITFAITFSVLSVASFVCPLWIQAAFWASFAFFTLGWWVAADLHL
                     AYFLEGQKDTAGLVKQHGHFKRLGLMFFSPEATFNDLKLSAAPLVPILVLCGVSFLGT
                     KALETAVPYTRYQIEWARAELV"
BASE COUNT          364 a          368 c          312 g          214 t
ORIGIN      
        1 gtaccagctc cgcccgtgcc cattcgatct gatagcgagt atagggcacg gctgtctcaa
       61 gcgcctttgt ccccagaaat gacactccgc acagaaccag gatcggaaca agcggtgcgg
      121 ctgacaactt cagatcgttg aaagttgctt ccgggctaaa aaacatcagc cccagacgtt
      181 tgaaatgacc gtgttgtttg accagaccag cggtatcttt ttgaccttca aggaaatacg
      241 ccaggtgcaa atcggcggcc acccaccatc caagggtgaa aaaggcaaat gacgcccaga
      301 aggcagcctg gatccagagt ggacacacaa aagaagcaac tgaaagaaca gaaaaagtaa
      361 tggcaaatgt aataaccacc ggtttcagtc gggcgggttt ttcgtcagtt ggtggatcag
      421 tacccagcac cgccgcgctc agacacacga ccgaaaccag cggcagaccg accaggatgg
      481 tcaacaacgg aaacacatcc ggctggacaa acttcccgcg aaccaccagc ccaaccacgt
      541 atggaacccc tgacaccacc attgccaaaa tcaaccagcc aagcgaacct ttgaacagat
      601 tgcccatgat tgatcgcgga taggtgtacc agcgccagac tgaaaatcct tcgtttgtaa
      661 tcattcgcgg gacggatgaa accagggcaa acaggccaat caaatacgat aaaacccccc
      721 aggccaccgg cgctttgatg aacagattgc gcagttcagg cagcagaaac aacggcacaa
      781 aggccagtgc cgggagcagc gcctggatca attgttgtgg gcggcgaatc acaaccttga
      841 tcgggtaggg ccagtttgca gcccgtgctt tttgggtact cgctccggca tccatccgaa
      901 accgacgcag ccactgatta cgattggcca tccagatcgt gagtgcggtg acccccaaca
      961 aacctgccag attcaggcac aacccgatca cccacgaaag cgagccggaa gccatcagtt
     1021 tgaccaccaa ccctggtgcc aggtactgaa aaaatgatga cttgagcagc ataaccaccg
     1081 acgttgcata ttcagaacca atcacgactg gaagcaggct caaggaaagg ccaaacccaa
     1141 gtgccgccag aaaagcagac cccggctgac gccatcccga cggaaccact ttccagctca
     1201 ctatggttaa aattgtctga atcatggcga tggtcagcat catcaggccg gtgccgcc
//
LOCUS       DAOQDO010002245         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_137834_length_1257_cov_4.786190, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002245 DAOQDO010000000
VERSION     DAOQDO010002245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137834_length_1257_cov_4.786190"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..986
                     /locus_tag="PKZ53_26365"
     CDS             210..986
                     /locus_tag="PKZ53_26365"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HNJ44035.1"
                     /translation="MNNQLPPIWVISLQRSTDRRAHITQHLTSHNLAFEICDAVDGQQ
                     LTASDLALYDARQSIQCLGREMTHGEIGCALSHLRLQQRMVNENIEEVLILEDDAVLK
                     FGFFEVLQRRALFPSDWELMLLCNTNGICSAWYHQPLFEHLAVVKFAGSVHSNAAYLL
                     KQSGARKLLAAGYPIRVPSDNLTGGVIRTQVRVYGISPVCVTTLHPFDLSQSTMPETR
                     QFRPRNRAKLGNLGWFRLTLRRLGRWMRHQYYRYNPLSIV"
BASE COUNT          349 a          288 c          307 g          313 t
ORIGIN      
        1 tgagggctga gggtatcggg ctaagggctg gaggaaatcc tcttttatac cattttgcaa
       61 tgaaagtatc gtattacggg aaggctaaag tattgaaaaa aatgtatttc cctgaaccct
      121 gaacccgata ccctgaaccc taattggtat tatcaacaac ttaacttttg gctcattgag
      181 ggtgatgatt gaacgtgtct cgtcacgcta tgaataacca gttgcctcca atttgggtca
      241 tcagtttaca gcggtcaacg gaccgtcggg cacacatcac ccagcatctg accagtcata
      301 atctggcgtt tgaaatctgc gatgcggttg atgggcagca gttaacggct tcggacctgg
      361 cactctatga tgcccggcag tcaatccagt gcctgggccg ggaaatgacc catggggaaa
      421 tcgggtgtgc gttgtcacat ctcagactcc agcaacgaat ggtgaatgaa aacatcgaag
      481 aagttctgat tttagaagat gatgcggttt taaagtttgg tttttttgaa gtattacagc
      541 gccgcgcact gtttccgagc gattgggagt taatgttgct gtgcaacacc aatggaattt
      601 gttccgcctg gtatcaccag ccattgtttg aacatctggc ggttgtgaaa tttgctggaa
      661 gtgttcacag caatgccgcc tatctgctca aacaaagcgg cgcccggaaa ttactggcgg
      721 ctggataccc gattcgggtt ccatcagaca acctgactgg cggggtgatt cggacacagg
      781 tcagggttta cggcatatcg ccagtttgtg ttaccacctt gcatccattt gatttaagtc
      841 agagcaccat gcctgaaacc cgccagtttc gcccacgaaa ccgggccaag cttggaaacc
      901 tggggtggtt tcgactgaca ctgcgccggc ttggccgctg gatgcgccat cagtattacc
      961 gttacaaccc attgtccatt gtttgaaaat gaagaatgaa gaatgaagaa tgaagaatga
     1021 agaatgaaga atggagaatg aagaatgtgt ctggtactta gagactatct caaaacgcta
     1081 aaataaaacc tgttttcaac acttagcctg gctaaattgg tgttttgaga tcgtttctta
     1141 gtacctggta cttaatgtct tgaaaccaat accttcgaag aaccaagaac caagaaccaa
     1201 gcaccaagca ccaagcacca agcaccaagc accaagcacc aagcaccaag caccaag
//
LOCUS       DAOQDO010002246         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138062_length_1256_cov_3.476270, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002246 DAOQDO010000000
VERSION     DAOQDO010002246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138062_length_1256_cov_3.476270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..>1256
                     /locus_tag="PKZ53_26370"
     CDS             458..>1256
                     /locus_tag="PKZ53_26370"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNJ44036.1"
                     /translation="MTALSLTQGVGYTKALILAMRPQQWTKNVLIFPALIFSERLMVW
                     SESAKVLAACAVFCFLSSSIYLLNDLMDLENDRAHPVKCHRPLASGQLPVPVGIATFV
                     VLAVLSLLAGWLLSKPFFFVALVYFFLQVAYSIRLKHIVILDVFCVAAGFVLRAIAGA
                     EVLQVFISPWLLICAMLLSLFLALGKRRHELLLLEEDAIAHRKILGEYSVDMLEQMIS
                     IVTASTLVCYALYTISSETGLKHPNLKYTIPFALYGIFRYLYLIHHKN"
BASE COUNT          286 a          302 c          306 g          362 t
ORIGIN      
        1 gaagattttg ctttcgccag gctgaacaaa gttatgtttg agcgaaaagg tgcgttcgat
       61 gccttccacc agccagagga tacagcctga cagaaagccg gttacaaggt agaaaaaaag
      121 tcgtgacttg acgtgtgcgg aaatgggtac tgcctttgaa ttcatagacc tacagaaaaa
      181 ctgcttggga ttattggggc taaagaagcc gggcataggg tttggggctg agcgctgaga
      241 aattcaatct gtttgaataa aaaatacccg tcaccaggtc cccaaagtaa tcctatatca
      301 ttttcaggct actgagacca agccgttttc actcactggg tgcaacgaga ggatcagacg
      361 ctgagaccca acccctttaa taaccgcgct tgcttgtgca aagtgatgaa acccggtagg
      421 atacccggtt ttcaaatctc atctaccatt catccttatg acagcacttt ctttgaccca
      481 gggcgttggg tacaccaaag ccctcattct tgcgatgcgt ccacagcagt ggactaaaaa
      541 tgtcctgatt tttccggcgc tgattttttc cgaacgcctc atggtctggt cggaatcagc
      601 caaggttctg gcagcgtgtg cggttttttg ctttttgagc agcagcattt atctactcaa
      661 tgacctgatg gatctggaaa acgaccgtgc gcatccggtg aaatgccatc gcccactggc
      721 ttcagggcaa ttgccggttc cagtcgggat tgccaccttt gtggttctgg ccgttttatc
      781 actgctggct ggctggctgc tctcaaagcc attttttttc gtcgcgctgg tgtatttctt
      841 tttacaggtg gcctactcga tccgtctcaa acacatcgtg attttagacg tgttttgtgt
      901 ggcggctggg tttgtcctcc gagcaattgc cggggccgaa gtcctgcaag tctttatttc
      961 accgtggctt ttgatttgtg ccatgttgct gtcactcttt ctggcgcttg ggaaacgccg
     1021 ccacgaactg ttgctgcttg aagaggatgc catcgctcac cgcaaaattt tgggcgaata
     1081 cagtgttgat atgctggaac aaatgatttc aattgtgacg gcttcgacgc tggtgtgtta
     1141 tgcgctgtac accatttctt cagaaactgg tctcaagcac cccaacctga aatacaccat
     1201 tccttttgcc ttgtatggga tttttcgcta tctgtatttg attcaccaca agaatc
//
LOCUS       DAOQDO010002247         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138072_length_1256_cov_3.184846, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002247 DAOQDO010000000
VERSION     DAOQDO010002247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138072_length_1256_cov_3.184846"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PKZ53_26375"
     CDS             <1..1015
                     /locus_tag="PKZ53_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44037.1"
                     /translation="EPFPESAPKTEIPLMIGIVRNEIKRQLSFGVTNTPADARLAKAQ
                     KDLEKIESSSGSTPAARSIASLNRIIGDLNKNIDQSLIPDAVLVTASSSGQAASSSSG
                     HDFDVDFKGDELDFKDQSGVEDVIPFQSLSEAQHDPRWQRAKDLGLLDDAQVRYWLQP
                     GYLAERGGKGIPTNLKGVIVELQAKDAAEAGHEDEFIEGDVEVFKGVKIVDPEDLTET
                     GRPRVMREIDVLVVRVLDDGQYVPIEVIEAKKQAGKKPKGLATDVRAKVKALREAQSK
                     GWRVIAGDEDITDLLVPNPEVQGLTAGPTGEYDVRLPASGEAEMLYDFINQYRYALSR
                     FWY"
     gene            1061..>1256
                     /locus_tag="PKZ53_26380"
     CDS             1061..>1256
                     /locus_tag="PKZ53_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44038.1"
                     /translation="MNAYAPQPPILKNSSATPSSSLMQSEGESLPLSFGPGLLSHQIN
                     QRSQNQTQLQLKRALNQSPQV"
BASE COUNT          356 a          292 c          315 g          293 t
ORIGIN      
        1 cgaaccattt cctgaatccg ctccaaagac tgagattcct ctgatgattg gcattgtcag
       61 gaatgaaatt aaacggcaac tgtctttcgg cgtcacaaat acacctgcgg acgccaggct
      121 ggcgaaagca caaaaggatt tagaaaaaat tgaatcatca tctggaagta ccccggcagc
      181 ccggagtatt gcgtctttaa atcgcattat tggcgatctc aacaaaaaca ttgatcaaag
      241 ccttatacct gatgctgtac tggtaactgc ttcgtcatct gggcaagctg cgtcatcgtc
      301 gtcaggccat gattttgatg tagatttcaa aggcgatgag cttgacttca aagaccagag
      361 cggagttgaa gatgtcattc catttcaatc actttcagaa gctcaacacg atccccgctg
      421 gcaacgcgcc aaagacctgg gcttgcttga tgacgcgcag gtcagatact ggcttcaacc
      481 tggctattta gccgaacgcg gtggcaaagg cattccaacc aatctcaaag gggtgattgt
      541 tgaacttcaa gccaaagatg ccgccgaggc tggccacgag gatgaattta tcgagggcga
      601 tgttgaagtt tttaaaggcg tgaaaattgt tgatcctgaa gatttaaccg aaaccggcag
      661 gccaagggtc atgcgagaaa ttgatgtttt ggtggttcgg gttttagacg atggccagta
      721 tgtcccaatt gaagtcattg aggcgaaaaa acaggctgga aagaaaccaa aaggattggc
      781 gaccgatgtc agagccaagg tgaaagcatt gagggaagcc cagtcaaaag ggtggagggt
      841 cattgccgga gatgaagaca ttaccgattt acttgtacca aatcctgaag ttcagggact
      901 gacggctggg ccaactgggg aatatgacgt tcgattacca gcctctggcg aagctgaaat
      961 gctctatgac tttattaacc agtatcgcta tgcgctctcc agattctggt attgattgga
     1021 agtgttaatt tggttcgacc tgaagcggag gaaacaaaga atgaatgcct acgcccctca
     1081 accacccatt ctgaaaaaca gctccgcgac accgtcttct tccctgatgc aatcagaagg
     1141 cgaatcactc ccgttgagtt ttggtccagg gcttttgagc caccaaatca accagcgttc
     1201 ccaaaaccag acccagttgc aactcaagcg ggcactcaat cagagcccac aggtgg
//
LOCUS       DAOQDO010002248         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138292_length_1255_cov_3.011667, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002248 DAOQDO010000000
VERSION     DAOQDO010002248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138292_length_1255_cov_3.011667"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKZ53_26385"
     CDS             complement(<1..999)
                     /locus_tag="PKZ53_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44039.1"
                     /translation="MVRKILGISAIAVVLVSLSFGLGSISARKTADTSLKPMSQLHQD
                     QVFHKTTGVNVSGSSSLLRPTVEHWRSLIKPVSRRQVLSVAAQPFSELRDGLLQVGGT
                     LEKQVAPGLDRRVTGVPENMEIEWVVAPWTDPTQIQLEFPEVEWVEYDRDQNLILHTA
                     AEDILIRDAKAWQEGSYQRMEVPVTVDFLGKGRVGITVGVYSTNDVLQIGLRVVTASR
                     AATGGGFATNATLTVTNGNDTGSGSLRNQIAAAAPGDTIQFSGVTTVTLTSAELVIDK
                     NLTINGGTNGVTITRSGATQFRIFNISSGTVSLTKLTISNGNNPGQAGGIQNSGTLT"
BASE COUNT          292 a          349 c          299 g          315 t
ORIGIN      
        1 cgtcagtgtc ccagaatttt ggatcccgcc ggcctggcct ggattattgc cattgctgat
       61 cgtcagtttg gtcaacgaga ccgttccgct gctgatattg aaaatgcgga actgtgtggc
      121 tccggaacgg gtaatcgtca ccccattggt accgccgttg atggtcagat tcttgtcgat
      181 caccagttcc gccgatgtca atgtcaccgt cgtcacgccg gaaaactgga tcgtgtcacc
      241 tggtgccgcg gcggcaatct ggttgcgcag cgatccagag ccggtatcgt tgccattggt
      301 caccgtcaac gtggcattgg tcgcaaaacc gccaccggtc gctgcccgtg atgccgtcac
      361 cactcgcaac ccaatctgga gcacatcgtt ggttgaatag accccaacgg tgataccaac
      421 tcgaccttta cctaaaaaat caacggtgac aggtacctcc atcctctggt atgagccttc
      481 ctgccaggct ttcgcatctc gaatcaggat atcttccgcc gccgtgtgca gaatcaaatt
      541 ctgatcgcgg tcatactcaa cccattcaac ctcaggaaac tcaagctgaa tctgagttgg
      601 gtccgtccag ggggcaacca cccactcaat ttccatattt tcgggaacac ccgtcacccg
      661 gcgatcaagc cctggggcaa cctgcttttc caacgtgcct cccacctgca gaagcccgtc
      721 ccgcaattca gaaaacggct gggccgcaac cgatagcacc tgccgcctgg aaaccggttt
      781 gatcaaggaa cgccagtgct ccaccgtcgg acggagcagg ctcgaagagc cgctcacgtt
      841 caccccagtt gttttatgaa acacctggtc ctgatggagc tgactcatcg gtttcagact
      901 cgtgtcagcc gtttttcggg ctgagatgga tccgagccca aaactcaaac tgacgagcac
      961 aacagcaatc gcactgattc ccaggatttt gcgcaccata ccagtgactc cctctcaaaa
     1021 tacagattat gaattacgag ttatgaaaac gttaactatc tgatattcaa agagataacc
     1081 ttttcatact acacttgact gcgttccagg cggtggttga attgaaaact gaatgaagtg
     1141 aacaggtttg tgttaagtac cttgccataa attttctgag atattgggtt tggtaagtgg
     1201 ttgattcttt tccgtgtttt ccgtgttttc cgtggttaaa atgtctggaa atttt
//
LOCUS       DAOQDO010002249         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138304_length_1255_cov_2.766667, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002249 DAOQDO010000000
VERSION     DAOQDO010002249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138304_length_1255_cov_2.766667"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1255)
                     /locus_tag="PKZ53_26390"
     CDS             complement(<1..>1255)
                     /locus_tag="PKZ53_26390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44040.1"
                     /translation="LGRAYLALTDYDQASTHLKMAEHAGMSSPELDYALGRTLGEFFR
                     RELARQVPAESEGERKQRLKRLEETFAIPALKHLEAYANSGGWMRAEEAAYVQGLIAF
                     YRRDYPQALTKAQDALAQRPWFAEAVLLIGDIHRTQGTEFLAIGQYEKSLEPLKLAQA
                     AYSQAGEISRSHPQVFESQAECTISIFEAAIFQLTLKPISEQEGRRLYDQSLSACNQA
                     LTVQPDNAHVWLTLARAKWRMADVMALFQRLQESEQLVDEALESAARAQQINPQLADA
                     NAVLGRIWFVKGLNANDRRQDVSPIVANSAHYLEQAIAQDPGNAQFYYYLGLTYFLMN
                     LAEKREGKDYTQANQKQIQMFEASIYYNPGSIKAMTLLVKAYSDRAWLEWQRGNSPLF
                     FFQQSFKYAQQARQMSEPDHRPVEAL"
BASE COUNT          290 a          326 c          312 g          327 t
ORIGIN      
        1 ccagggcttc aactggccga tggtcgggtt cactcatttg cctggcctgt tgggcatact
       61 taaatgactg ctgaaaaaag aaaaggggtg aatttccccg ttgccattcc aaccaggctc
      121 gatctgaata ggccttcact aacagtgtca tcgcttttat tgaacctgga ttgtaataaa
      181 tggaggcttc aaacatctga atttgtttct ggttggcctg ggtgtaatct tttccttccc
      241 gtttctcagc taaattcatt aaaaaatagg ttagccccag gtagtagtaa aactgggcgt
      301 ttcctggatc ctgtgcaatc gcctgttcca ggtaatgcgc tgagttagca acaatgggtg
      361 agacatcttg cctcctgtca tttgcattca gccctttgac aaaccagatc ctccccaaca
      421 cggcatttgc atccgcgagt tgcggattga tttgttgggc tcgtgctgct gattcaagtg
      481 cttcatcaac cagttgctca gactcctgga gtcgttggaa aagtgccatg acatccgcca
      541 tccgccattt ggcccgggcc agtgtcaacc agacgtgcgc attatctggt tgaaccgtga
      601 gcgcctggtt acacgcggaa agagactggt catacaaacg acggccttct tgctcggaaa
      661 tgggcttcag ggtgagttga aaaattgccg cttcaaaaat tgaaatggtg cattccgcct
      721 gagactcaaa cacctgtgga tggctccggc tgatttcacc tgcctgggaa taagcggctt
      781 gagcaagttt gagaggttca agcgattttt catattgccc aatcgccaga aactcagttc
      841 cctgggttcg atgaatatcg ccaatcaaaa gaacagcttc ggcaaaccat ggtctttggg
      901 ccagtgcatc ctgcgctttc gtcagcgcct gggggtagtc acgccgataa aaggcaatca
      961 acccctgaac ataggcggct tcttcggcac gcatccaacc gccggaattg gcataggctt
     1021 ccaggtgttt taaagctgga atcgcaaagg tttcctcaag tcttttcaat cgctgcttcc
     1081 gctcaccctc ggattctgcc gggacctgtc gagccagttc acgtcgaaaa aattcaccga
     1141 gtgtgcgacc aagcgcataa tccagttcag ggctgctcat cccggcatgt tcggccatct
     1201 tcaggtgggt acttgcctga tcataatcgg tgagtgccag atacgcccgg cccag
//
LOCUS       DAOQDO010002250         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138498_length_1254_cov_2.977481, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002250 DAOQDO010000000
VERSION     DAOQDO010002250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138498_length_1254_cov_2.977481"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKZ53_26395"
     CDS             <1..280
                     /locus_tag="PKZ53_26395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44041.1"
                     /translation="FILHSSFFILHSQFFILHSNPPAYAGGTDFLIFRHLAQHKPVDW
                     VSVVLYGVRIIPKEPHTSSIHASPFSAQTTSTITVFRTGLIRLEGICL"
     gene            277..>1254
                     /locus_tag="PKZ53_26400"
     CDS             277..>1254
                     /locus_tag="PKZ53_26400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ44042.1"
                     /translation="MNYMTKEMLKRYGRMALGQIFPPIFLNILVTSVCDMRCVHCFFT
                     EELDDKDRKKLQMKTNNIVRISETLGGNLGVLVIAGGEPFTRIDLPQIARAFYENNKL
                     ESIYLMSNGQIQKRILPDVERIRKECPNLNVTVALGIDGLETEHDKIRQKPGSWAKAI
                     DTAFKLKELKKEYGGIDIQTCTCLMNSNQDRIFEWYDFLKHELKPDKINVNYIRPPSA
                     RPEELNIDLNRYQKLSRQILDDSCSASLKNIYTGDSGFFKAAIDIYMHELIAKTQVEN
                     KAQLRCWAGTAGGVIYDEGTVSSCENLPAIGNLRDFDWDFGALWASQAMA"
BASE COUNT          336 a          311 c          289 g          318 t
ORIGIN      
        1 cttcattctt cattcttcat tcttcattct tcattctcaa ttcttcattc ttcactcaaa
       61 cccacccgct tacgcaggtg gcactgactt cctcattttt cgccatctgg ctcagcacaa
      121 accagttgac tgggtttcag tggttctcta tggtgtgcgc atcataccaa aagaacctca
      181 tacttcatca attcacgcat cacctttttc agcccagaca accagtacca tcacggtatt
      241 tcggacaggt ctgatacgcc ttgaaggaat ttgtttatga actatatgac caaagaaatg
      301 ctcaaacgct atggacgtat ggcgttgggg cagattttcc cgccgatctt tctcaatatt
      361 ctcgtgactt cagtgtgtga tatgcggtgc gtacactgct ttttcaccga agaactcgat
      421 gacaaagacc gcaaaaagct ccagatgaaa accaataaca tcgttcgcat tagcgaaacg
      481 ctgggaggga atctgggcgt gctggtgatt gccggcggtg aaccgtttac ccgtatcgac
      541 ctgccacaga ttgcgcgcgc cttttatgag aacaacaagc tggagtcgat ctacctgatg
      601 tcaaatggtc agatccaaaa gcggattctg ccggatgtcg aacgtatccg gaaggaatgc
      661 ccaaatctga atgtgaccgt ggccctggga attgacgggc tggaaaccga gcacgacaaa
      721 atccgccaaa agccagggag ctgggccaag gccattgata cggcctttaa actcaaggaa
      781 ctcaaaaaag aatatggtgg cattgatatt caaacctgca cctgtttgat gaattcaaac
      841 caggatcgca tttttgagtg gtatgacttc ctcaaacatg aactgaagcc agacaaaatc
      901 aacgtcaact atattcggcc cccttcagcc cgcccggaag aactcaacat tgatctgaat
      961 cggtatcaga aactctccag gcagattctg gatgattcgt gcagcgcgtc gttgaaaaat
     1021 atctacactg gcgattcagg atttttcaaa gcggcgattg atatctatat gcacgaattg
     1081 attgccaaaa cgcaggttga aaacaaagcc caattgcggt gctgggctgg aacggcgggc
     1141 ggtgtaatct atgatgaagg cacggtttca agctgtgaaa acctgcctgc catcggcaat
     1201 ctccgcgatt ttgactggga ttttggcgcg ctgtgggcat cacaggccat ggct
//
LOCUS       DAOQDO010002251         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138594_length_1254_cov_1.895746, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002251 DAOQDO010000000
VERSION     DAOQDO010002251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138594_length_1254_cov_1.895746"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKZ53_26405"
     CDS             <1..477
                     /locus_tag="PKZ53_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C14"
                     /protein_id="HNJ44043.1"
                     /translation="GHGHWDEGLKQGFWLPKNAERLKRAEWLPNGSLRDYIGGIPARH
                     TILISDSCFSGSIFKSREAFAQPTAEMKELYQLPSRQAMTSGALQSVPDKSVFVEYLV
                     KRLEQNQEPYMSAESLFSSLRKAVINNSPNGQVPQFGEIREAGDEGGDFIFVKRTR"
     gene            652..>1254
                     /locus_tag="PKZ53_26410"
     CDS             652..>1254
                     /locus_tag="PKZ53_26410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44044.1"
                     /translation="MRPGSRQELFSKTFELAYFIVPEESAALKVTEQAFTELDRTYVE
                     QKKRSYYKADDRYKMSLSRHQLLQRLVFEHTEPYEIAQEQQAPHTLDEATLFVRYIKH
                     LVRFSLRQNTFFMTVGLGRLLYTYSTSDVVKVYGFLTNHEKDVMQFRRAKGKILESLI
                     ARFGNLIEVTTVNQENRITSHPNPPSALIDLALDSLRQFTP"
BASE COUNT          337 a          314 c          299 g          304 t
ORIGIN      
        1 ggtcacggcc attgggacga aggactcaaa caagggttct ggttgcccaa aaatgctgaa
       61 cgactcaaac gggcagagtg gctgcccaat ggttctctgc gggactacat tggcgggatc
      121 ccggctcgtc ataccatcct gatttcagat tcgtgtttta gcggaagtat cttcaaaagc
      181 cgggaagcct ttgcccaacc caccgctgag atgaaggagt tgtaccaact gcccagtcgc
      241 caggccatga ccagcggagc tttgcaatcc gtcccagaca aaagcgtgtt tgttgaatat
      301 ctggtcaaac gcctggagca aaaccaggaa ccctacatgt cggccgaatc ccttttcagt
      361 tcattgcgca aagcggtgat caataacagc cccaacggtc aggtaccaca gtttggcgaa
      421 atccgcgaag cgggcgacga ggggggggat tttatcttcg tcaaacggac cagataacgg
      481 tttccttttc aaggtgggtt ttcgatgatc atctccgcct ttttcaagtg aggcaggcca
      541 tcagagtctg aaaggggttt cagattctga tacgcagaaa aaaccggttg ttgcgtacaa
      601 ttgtcaccag cccctcatcc tacataaccc aaaaggtaaa gtttaaagac catgagacca
      661 ggatcacggc aagaactctt tagcaagacc tttgagttgg catatttcat agtgcctgag
      721 gaatcggctg ctctcaaagt gacagaacag gccttcaccg aactggatcg tacctatgta
      781 gaacaaaaaa agcgttccta ttataaagcc gatgaccgtt acaagatgtc tttgtcacgc
      841 catcaactcc tgcaacgcct ggtgtttgag cacactgaac cctatgaaat tgcgcaggag
      901 cagcaggctc cacacaccct cgatgaagca actctgttcg ttcggtacat taaacatctg
      961 gttcggttct cgttgcggca aaacaccttt tttatgaccg tgggactggg ccgtttgctt
     1021 tacacttact caaccagtga tgtggtcaag gtttatggat tccttacaaa tcatgaaaaa
     1081 gacgttatgc aatttcggcg ggcaaaggga aaaattttgg aatcgttaat tgcacgattt
     1141 ggcaatttga ttgaggtgac cacggtcaat caggaaaacc gaattacctc gcatcccaat
     1201 ccgccatcag cccttatcga tttggcactc gactcattac ggcaatttac gccc
//
LOCUS       DAOQDO010002252         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138682_length_1253_cov_3.291319, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002252 DAOQDO010000000
VERSION     DAOQDO010002252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138682_length_1253_cov_3.291319"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKZ53_26415"
     CDS             <1..666
                     /locus_tag="PKZ53_26415"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNJ44045.1"
                     /translation="KRVFTIVTICLVLWGLPLLALISWQGHHHLHVQEYLFFSQAALV
                     TFGGAYSVLAYVTQAAVGSHGWLSQSQAVDGLALAETTPGPLIMVLQFVGFMAGWNHP
                     GMFSPLASAIIGALITTYTTFLPCFFFIFLGAPYIESLRGNQHLTATLSGITAAVVGV
                     VLNLALVFGSAVIWPHGFSAATNWWALLWSLVAFAALYRFKIDVLWVVLVAGSIGLLV
                     SVG"
     gene            771..1196
                     /locus_tag="PKZ53_26420"
     CDS             771..1196
                     /locus_tag="PKZ53_26420"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNJ44046.1"
                     /translation="MPKFSLRGFKTDRQGASHVLGELETAIMELLWQQPQQTVSEVEN
                     RLAHNRTIAHTTVLTTLDRLHRKGYLTREKEGKAFVYAPAYTREEFERGMAEEVLSAI
                     LKGIGEPALSTFVDMVSEDEAQLKKLEALIQEKRKGKRP"
BASE COUNT          278 a          336 c          335 g          304 t
ORIGIN      
        1 aaacgtgtct tcacgattgt gaccatctgc ctggtgttat ggggactgcc gctgctggcc
       61 ttgataagct ggcagggaca ccaccatctg cacgtccagg agtatctgtt tttcagccag
      121 gcggcactgg tgacatttgg cggcgcctat tcggttctgg cctatgtgac ccaggcggca
      181 gtcgggtcac acggctggct gagccagtct caggcagttg acgggctggc cctggcagaa
      241 accacacctg gcccattgat tatggtactt cagtttgttg gatttatggc cggctggaat
      301 catccaggga tgttctcacc gcttgccagc gccattatcg gagcgctcat caccacctac
      361 accacctttc tcccctgctt tttctttatt tttctggggg cgccttacat cgagtcactt
      421 cgtggaaatc agcacctgac cgccaccctg agtggtatca cagccgccgt cgtcggcgtc
      481 gtcctcaatc tggcgttggt gttcggcagc gccgtcatct ggccgcatgg tttttcagcc
      541 gcaaccaact ggtgggcgct cctgtggagc ctggtggcat ttgcggcgct atatcggttc
      601 aaaattgacg tgctgtgggt ggttctggtc gcgggaagca ttgggctcct ggtatcagtg
      661 ggttgagagc atcggtgagt ttggagaaac taaacaacta ttgacaatag tttttcagtt
      721 cactagagtg atcactctcc gtcatttttt catagcataa agccaccacc atgccgaaat
      781 tttcactcag agggtttaaa accgaccgcc agggcgccag ccacgtgctg ggtgaattgg
      841 agaccgctat tatggagctt ctctggcagc agccccagca aacggtctcc gaagtggaaa
      901 accgccttgc ccacaatcgc acgattgccc acaccacagt attaaccacg cttgaccgac
      961 tccaccgcaa aggctatctg acccgcgaaa aagaaggaaa agccttcgtc tatgcacctg
     1021 cttacacccg cgaggaattt gaacgcggaa tggccgaaga agtcctgagt gcgatcttga
     1081 agggaattgg cgaaccggca ctctcaacct ttgtggatat ggtcagtgaa gacgaagccc
     1141 agttaaaaaa actggaagcc ttgattcagg aaaaacgaaa aggtaagcgg ccatgatgat
     1201 ttcaccgtgg gcactcctgc tggttggaat tgaaaatttt gtcctgatca aca
//
LOCUS       DAOQDO010002253         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138959_length_1252_cov_2.436926, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002253 DAOQDO010000000
VERSION     DAOQDO010002253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138959_length_1252_cov_2.436926"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKZ53_26425"
     CDS             <1..277
                     /locus_tag="PKZ53_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44047.1"
                     /translation="HPSSFIPHPFDCPKPDVFHPKPDVFHPKPDVFRPLLFSPMVSRF
                     SGFPKFPHCRTPVFCVFLRFSFAPILSLFFFLYVKPKTNHCRELENV"
     gene            231..995
                     /gene="tpiA"
                     /locus_tag="PKZ53_26430"
     CDS             231..995
                     /gene="tpiA"
                     /locus_tag="PKZ53_26430"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNJ44048.1"
                     /translation="MSNRKPIIAGNWKMFKTVSESVATALDLKSKTFNANHCEVVIAP
                     VFTSLKTVADRLEGSHIKVAGQDVAAEGPEGAFTGEISAEMLADAGCTHVIIGHSERR
                     QYYHETNDRVARKVQRALAVGLTPILCIGETLAQREAGEAEKVVTEQLIEGTATLTAP
                     DLFRIVAAYEPVWAIGTGHAATPDTAQAMHALVRSVLSEKFGTEAAQQLRILYGGSVK
                     PENIAEYMEQPDIDGALVGGASLKADSFARIVFYQQ"
     gene            1074..>1252
                     /gene="secG"
                     /locus_tag="PKZ53_26435"
     CDS             1074..>1252
                     /gene="secG"
                     /locus_tag="PKZ53_26435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNJ44049.1"
                     /translation="MQWYVYILYAIFFLACMILIGVVLLQPGKSDAAAVFGGGSQTAF
                     GARGAQKPLERITFGA"
BASE COUNT          271 a          355 c          304 g          322 t
ORIGIN      
        1 tcatccctca tccttcatcc ctcatccctt cgattgcccc aagcccgatg tcttccaccc
       61 caagcccgat gtcttccacc ccaagcccga tgtcttccgc ccgcttctct tcagcccgat
      121 ggtttcaagg ttttccggtt ttccaaaatt tccccattgc cgaaccccgg tcttttgcgt
      181 attcttgcgt ttttcctttg cccctatcct ttcgctgttc tttttcctgt atgtcaaacc
      241 gaaaaccaat cattgccggg aactggaaaa tgtttaagac ggtttccgaa tcagtcgcca
      301 cggcgctgga tctgaaatca aaaacgttta atgctaacca ttgtgaagtc gtgattgcac
      361 ccgtcttcac ctctttgaaa accgttgctg atcgtcttga aggaagccac atcaaagttg
      421 ccgggcagga tgtggccgcc gaaggccccg aaggggcatt tactggcgaa attagcgctg
      481 aaatgctggc ggatgcgggc tgtacccacg tcatcattgg tcactctgaa cgtcgtcagt
      541 actaccatga aaccaatgac cgggtcgctc gaaaggttca acgcgccctg gccgttgggc
      601 tgacgccgat tctgtgcatt ggagaaacac tggcgcaacg cgaagccggt gaggcagaaa
      661 aagtggtcac tgagcagtta attgaaggaa ctgctacctt gacagcccct gaccttttcc
      721 gtatagtcgc cgcctacgaa cctgtctggg ccatcggaac cggccatgca gccacccctg
      781 acaccgccca agccatgcac gccctcgttc gatccgttct cagtgagaaa tttggtaccg
      841 aagccgcaca gcaactgcgg attctgtatg gcggcagcgt caagccggaa aacatcgctg
      901 agtatatgga acaacccgat atcgatggtg ccctggtggg tggagccagc ttgaaagccg
      961 attcatttgc caggattgtg ttctatcaac agtaatctgt tggccagatc ggcgtcagtt
     1021 ttgactcacg acccagacca gcagacattc attgggattg cctttaactt attatgcagt
     1081 ggtacgttta cattctttac gctattttct ttctggcctg tatgatcctg attggggtcg
     1141 ttttgctcca acctggaaaa agtgatgccg ccgctgtttt tggcggtgga agccagacag
     1201 cctttggggc tcgcggggct cagaaaccgc ttgaacgaat cacatttggc gc
//
LOCUS       DAOQDO010002254         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_138971_length_1252_cov_2.308271, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002254 DAOQDO010000000
VERSION     DAOQDO010002254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138971_length_1252_cov_2.308271"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKZ53_26440"
     CDS             complement(<1..443)
                     /locus_tag="PKZ53_26440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44050.1"
                     /translation="MKPLMKYATVALVILLGWAMSAQAETLKRRYTLPFKGGAMRTAS
                     RSQALNQAKQRFLADFLKDKFSETVIQEHQEDIDNALDPPTDYVLDFQVKSEKINPDE
                     TQITLTVEGNLDLPKMVAALVNNDVLSFGDKPPRVMVIPAGSFTDP"
     gene            complement(612..>1252)
                     /locus_tag="PKZ53_26445"
     CDS             complement(612..>1252)
                     /locus_tag="PKZ53_26445"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNJ44051.1"
                     /translation="VRIGKALSAQYVILARCLGVENKSKSGGFAGIKVGTSEMTCAFQ
                     MQMVNTETTEILASESYNKKKKTGSSFSGVLGASKSADEETLKAFTEATIEAANDFAE
                     KLALATPLEGLVVLVKADRVAINAGADKGVAEGQEFEVMIEGDPITDENGNTIGYDRT
                     KVGVIRVVQVEPKLAWCTLVQTFDAKGTPDASPSTSRIEKFHKVRQIGKGRH"
BASE COUNT          292 a          324 c          300 g          336 t
ORIGIN      
        1 ggatcggtaa aacttccggc gggaatgacc atcacccgtg gtggcttgtc accaaagctc
       61 aggacatcgt tgttgaccag ggcggccacc atttttggta aatccaggtt gccttcgacg
      121 gtgagggtaa tttgggtttc gtcagggtta attttttctg atttcacctg aaaatcgagc
      181 acataatcag ttggtggatc aagggcatta tcaatgtctt cttgatgttc ttgaataacg
      241 gtttccgaaa atttgtcttt aagaaaatca gctaaaaagc gttgtttggc ttgattgagc
      301 gcctggctgc gggaggcagt gcgcatcgct ccacctttaa acggcagggt gtagcgacgt
      361 ttgagggttt cagcctgcgc tgacatagcc catccaagca ggatgacaag cgcgacggtt
      421 gcatacttca tcaatggttt cacaactatt tcctccttcg gaaaataaca aacgggcaca
      481 gtctgaaact gtgcccttta tgaccaataa cttctatcct ttcaacaagg cacaaggctg
      541 gagttcatca aatacacaag tggaaccttc ctggttttcc cgacaccctc agccccaagc
      601 ccgaaacgat attaatggcg gcctttgcca atctggcgga ccttatggaa tttttcgatc
      661 cggctcgtgc ttggtgaggc atccggtgtg cccttggcgt caaaggtctg taccagcgta
      721 caccaggcca gtttgggttc gacctgaacc acgcgaatca ccccgacttt ggttcggtca
      781 taaccaatgg tattgccatt ttcatccgtg atcgggtcgc cttcaatcat gacttcaaat
      841 tcctgacctt cggcaacacc tttatcagcg ccagcgttga tcgccacccg gtcggctttg
      901 accagtacca ccagaccttc aaggggggtg gccagagcca gcttttcggc aaaatcattg
      961 gcagcctcga tagtggcttc agtaaatgct ttgagcgttt cttcatcagc actctttgag
     1021 gcccccaaca cgcctgaaaa cgaagacccg gtctttttct ttttgttgta ggattccgag
     1081 gccaaaattt ccgtcgtttc ggtgttgacc atctgcattt gaaacgcaca ggtcatttca
     1141 ctggtgccga ctttaatacc agcgaatcca cctgattttg atttattttc cacacccagg
     1201 cagcgggcga ggatgacgta ttgagccgac aacgccttgc cgatacgaac ag
//
LOCUS       DAOQDO010002255         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_139157_length_1251_cov_2.705686, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002255 DAOQDO010000000
VERSION     DAOQDO010002255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139157_length_1251_cov_2.705686"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKZ53_26450"
     CDS             <1..515
                     /locus_tag="PKZ53_26450"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HNJ44052.1"
                     /translation="VLTDEKFFNGSLNLLNDVVRNTKLPVLRKDFVLDPLQVYEARLA
                     GAEAVLLIAKILTDAELATLSGLTRQLGMSAVIEVQTQAELERAMRLRPRVVLINNRN
                     LDTFEISFETTRRLAPLIPKDTLTISASGIQSRSDIEELLPFCTRFLVGTALMQTTTL
                     SQTLEELTSI"
     gene            512..1132
                     /locus_tag="PKZ53_26455"
     CDS             512..1132
                     /locus_tag="PKZ53_26455"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNJ44053.1"
                     /translation="MTTKVKICGLTSIHDAEMVVAAGADYLGLIFVKESQRCLSMTIA
                     EQIRKAVSGRISIVGVFRNAPATFVAEAARRLALDFVQLHGHESPAYCQSLSTPVIKV
                     LELTDQLNQDSIDQYSSCASYLLFDRPKRTHQPPAVLDPIAILRGLELNLPFFFAGNL
                     SPETVRTPIDQLNPFGIDVASGVEAAPGIKDPDKVRAFCEACRQSE"
BASE COUNT          296 a          348 c          304 g          303 t
ORIGIN      
        1 cggtgttaac cgacgagaaa tttttcaatg gcagtctcaa tttactgaat gatgtggtca
       61 gaaacaccaa attaccggtg ctgcgcaagg attttgtgct tgacccgctg caagtctacg
      121 aagcgcggct ggccggggct gaagcggtct tgctcatcgc gaaaatcctg accgatgccg
      181 aactggcaac gctctctgga ttaacccggc aactcgggat gtctgccgtg atcgaagtcc
      241 aaacccaggc ggaacttgaa cgcgccatgc gactcaggcc ccgcgttgtg ctcattaaca
      301 accgcaatct ggataccttt gaaatttctt ttgaaacgac ccgtcgcctg gcaccgttga
      361 tcccaaaaga cacgctcacg atttcagcca gtggcattca aagccgatcc gatattgaag
      421 aactcctccc cttttgtacc cgatttctgg ttggaaccgc tctgatgcaa accaccacgc
      481 tttcccaaac cttagaggag ctgacctcaa tatgacgacg aaggtaaaaa tctgcggact
      541 tacgtccatt cacgatgctg aaatggtcgt cgcggcaggt gctgactacc tcggattgat
      601 ctttgtaaaa gaaagccagc gctgcctttc gatgaccatt gccgaacaga ttcgcaaagc
      661 ggtttccggg cgaatctcaa ttgtcggtgt gtttcgcaat gccccggcca catttgtggc
      721 cgaagccgcc cgccgtctgg cactcgattt tgtacaatta cacggccacg aatcaccagc
      781 ctattgccag tcactttcca ccccggtgat caaagtcctg gaattgaccg accaactcaa
      841 ccaggactcc attgatcaat attccagttg tgccagttat ctgttgtttg atcgccccaa
      901 gagaacacat caaccaccag ccgtgcttga cccaattgcc atccttcgcg ggttggagct
      961 aaaccttccc ttcttttttg ccgggaatct gtcacccgaa acggttcgga cgccgattga
     1021 ccagctcaat ccatttggaa ttgacgtggc cagcggtgtc gaggcggccc ccggtatcaa
     1081 agacccagac aaagtgcggg ctttttgcga agcctgccgt caaagcgaat gaagaattga
     1141 gaatgaagaa tgaagaaact gcctggtgcc tggtgcctgg tgcttggtgc ttggtgcttg
     1201 gttcttggtt cttggttctt ggttttgaaa acccggaacc ctaaacccta a
//
LOCUS       DAOQDO010002256         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_139172_length_1251_cov_2.531773, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002256 DAOQDO010000000
VERSION     DAOQDO010002256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139172_length_1251_cov_2.531773"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..864
                     /locus_tag="PKZ53_26460"
     CDS             289..864
                     /locus_tag="PKZ53_26460"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNJ44054.1"
                     /translation="MSANRGQYYTLEEYFAVEQVGNTRYEYWDGDIVCMSGGSEAHGR
                     ICGNVYFALRLQTGGGPCQAFTSDVAVKTPTLPPYRYPDTSIACGTAVFENINGVDVL
                     VNPVLIVEVLSPQTEKADRAAKFEAYKAIDTFREYLLIAQHEPHLTHFVKQETGEWRR
                     FDTADLTATVELEAVGCILVVADVYATVVFP"
     gene            963..>1251
                     /locus_tag="PKZ53_26465"
     CDS             963..>1251
                     /locus_tag="PKZ53_26465"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HNJ44055.1"
                     /translation="MVSLSPNNLLVIFDLDETLVFTRMVRLYSIYNDPGDFQIDLGDV
                     VFDVFVRPGARKLIAYCQERFQVGVWSSAKEDYVQALAAQLFDLEKVAFIRH"
BASE COUNT          304 a          318 c          298 g          331 t
ORIGIN      
        1 ttgatctcca ggaattcacc tgccaacttg agattttgat ccagcgccct gccgggcgcg
       61 aaccctgtgt ggcttgcctt ccggtgggtg cgcctcaaag caggctgccc accggctact
      121 ttccggtgcc ctccgggcaa gatatattgt caatctcatt gaacctcttt caccagtgac
      181 cataaaaaat ctcaaccatt gactccgtaa cctctttcgg cttttatcca aataatctaa
      241 ttgatacttt ttaaagcatt tccatttaga tgaaagaagg agtggcttat gtcagccaat
      301 cgaggacagt actacacact ggaagagtat ttcgcggttg aacaagtcgg aaatacccgg
      361 tatgaatact gggatggcga catcgtctgt atgagtggcg gaagcgaagc ccacggacgc
      421 atctgcggaa atgtctattt tgcattgcgg ctgcaaactg gtggaggccc ctgccaggcg
      481 tttaccagcg atgttgcagt caaaacacca accttacctc cgtaccgtta ccctgatacc
      541 agcattgcct gtggaacagc cgtgttcgaa aatatcaatg gggttgatgt tctggtaaat
      601 ccggtgttga tcgttgaagt actgtctcca caaacagaaa aggctgaccg agcggcgaaa
      661 tttgaagctt acaaagccat tgataccttt cgcgaatatc ttctgattgc ccaacatgaa
      721 ccgcacctga cacactttgt aaagcaggaa actggtgaat ggcggcgatt tgacacggca
      781 gatctgacgg ccacggtgga actcgaagca gttggttgca tactggttgt cgctgatgtc
      841 tatgccaccg ttgtttttcc ctgacaagaa ccgtatgttt cacaccacat caattacacg
      901 gcaactcttc gggcctaaac caattcttga accctgaacc ccgaaccctg aaccctacct
      961 gtatggtttc cctctcgccc aacaacctgc tggtcatttt cgatctggac gaaaccctgg
     1021 ttttcacccg aatggtccgc ctgtattcga tttataatga tccaggggat tttcagattg
     1081 atttgggaga tgtggttttt gacgtgtttg tccgtcctgg agcgcgaaag ttgattgcct
     1141 attgccagga acggtttcag gtcggagtct ggtcaagtgc aaaagaagat tacgttcagg
     1201 cgctcgccgc acaactcttt gatctggaga aggttgcctt tatccggcac c
//
LOCUS       DAOQDO010002257         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_139586_length_1249_cov_2.768844, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002257 DAOQDO010000000
VERSION     DAOQDO010002257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139586_length_1249_cov_2.768844"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..844
                     /locus_tag="PKZ53_26470"
     CDS             131..844
                     /locus_tag="PKZ53_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNJ44056.1"
                     /translation="MKTRQWFKLSLILIVAAAALTIYGVAQAQQKSAPLPQHPPAPGD
                     GKNLVIQLCDGETNLEMKGVKPDERLSPDQAQTAANTLMSMWFAKTDPNVAEAWKKEM
                     AEALAQKKGSEKAAPASQSEQDGVFTARDRQVWERELAREIQYGNEIFHSDKLVGSTN
                     GVSCAMCHPNAANTHPETYPKYQIQLQRVALLRDMINWCIENPSRGKKLDADDPKMRA
                     IEAYIMAQRKGVAMEYGKH"
BASE COUNT          355 a          343 c          296 g          255 t
ORIGIN      
        1 gaaagcattg attcctaagc actaagcacc aggcaccagg ttctaagcac caggttctaa
       61 gcaccaggca ctaagcacca ggcactggtt cctcatcggt tccacaagga gaaaaaaagg
      121 agaacttcca atgaaaacac ggcagtggtt caaactgagt ttaattctga tcgtggcggc
      181 ggcggcactg accatttatg gtgtagccca ggctcaacaa aaatcagcgc cgcttccaca
      241 acatccgcca gcgcccggcg acggcaaaaa cctggtgatt cagttgtgcg acggtgaaac
      301 caaccttgaa atgaaagggg tcaaacccga cgaacggctc tcacctgacc aggcacaaac
      361 cgcagccaat acactgatgt caatgtggtt tgccaaaacc gatcccaacg tggccgaagc
      421 ctggaaaaaa gaaatggctg aggcactggc ccaaaagaaa ggctcggaaa aagctgcacc
      481 ggccagccag tcagaacagg atggggtgtt tacagcccga gaccgtcagg tgtgggaacg
      541 tgaactggcc cgcgaaatcc aatatggcaa cgaaattttc catagtgaca aacttgtcgg
      601 cagcaccaat ggcgtctcct gtgccatgtg ccatcccaac gccgccaaca cccatccaga
      661 aacctatccc aagtaccaga ttcagctcca acgcgtcgcc ctgctccgcg acatgatcaa
      721 ctggtgcatt gagaatccct ctcggggcaa gaagcttgac gcggatgacc caaaaatgcg
      781 cgccatcgaa gcctacatca tggcccaacg caaaggcgtc gccatggaat atggcaagca
      841 ttgaaagact ggggctgaag aaaacgggct gaaggctgaa ttacccattt tcttcagccc
      901 taaaatcttc atcaaaggtt ttcgagatca ataattgtaa gggactgtaa aagattgact
      961 tcccgttttt aatttgggag cgcaggcatc ttgcctgcat tttccggctg atggcgggtg
     1021 ggatacccgc gctcgccgga actcattttt taacgacctc taacggttcc aactgggaaa
     1081 gtccagtcag cgatgacaaa ttggaaaagg tattttcaac acaagcagag ccctcctgcc
     1141 cgcacgatac tccacaacca attccgcttt ccctggttga ttgtgcttga atatcgtttc
     1201 acccctcaat ggattgttga ccaccaccaa tggaatactt tttcaactt
//
LOCUS       DAOQDO010002258         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_139728_length_1249_cov_1.642379, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002258 DAOQDO010000000
VERSION     DAOQDO010002258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139728_length_1249_cov_1.642379"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKZ53_26475"
     CDS             complement(<1..181)
                     /locus_tag="PKZ53_26475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44057.1"
                     /translation="MCSLNISVVSLEGKSDRRSPGSIGDGGPRMVIQGFKIRRLMRGE
                     DASAWVRSLEAGDWLG"
     gene            284..>1249
                     /locus_tag="PKZ53_26480"
     CDS             284..>1249
                     /locus_tag="PKZ53_26480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44058.1"
                     /translation="MFGENGVVDALDDTVNTILDQNSDQKGKPGDGLLRRDVDVETQP
                     VTAPTDAAPARTGESPHGAMPTNDGYEYFCGNINTTTWRVTVPDEVNPTQTNYTTLLN
                     GAGITQTMSGVTTGAGTAPAASQTGAINWRIVDSAKTGRIRAFRRAVRIVNGWDIPRW
                     YLSQDGPVPRHFSGMSLICENPVYIYGNLNSIGVQEQPSTIVDPDSGLPVGGPTASRR
                     FLQTGNRNMPLVPGNFNSNPPVTGGADDALLHGPMSIMADAVSIHSRAFQDSRMFICS
                     RGWGFRPQNRLRRYAMETTVKAAWLTGQPKAGVQTTSQQTSDDNGD"
BASE COUNT          304 a          364 c          317 g          264 t
ORIGIN      
        1 accccagcca atcgcccgct tcgagcgatc gcacccacgc cgaggcatcc tcgccgcgca
       61 tcagccgccg gatcttgaag ccctggatca ccatgcgcgg cccgccatca ccgatgcttc
      121 cgggcgacct tcgatcgctt tttccttcca aactgacaac gctgatattc agcgaacaca
      181 ttcgcctcgg gcccctcgac tgcctgtcga accaggagct ccgtgtcatg gctctgatcg
      241 gcgcggggat gacaacaacg acggggttta tgagtttaac gacgtgtttg gcgaaaacgg
      301 agtggttgat gctctggacg atacggtcaa taccattctt gaccaaaatt cagatcagaa
      361 agggaaacct ggtgatggtc ttctccgccg tgacgtagat gtcgaaaccc aacctgtaac
      421 ggcaccaact gatgcggccc cagcccgaac tggtgaatca ccgcatggtg ccatgccaac
      481 caatgacgga tatgagtatt tctgcggtaa catcaatacc accacctggc gggtcaccgt
      541 gccagatgaa gtcaatccaa cccaaacaaa ttacaccaca ttgctcaacg gagctggcat
      601 tacgcagacc atgtcgggcg taaccactgg ggctggaacc gcaccagcgg caagccagac
      661 tggtgccatc aactggcgaa tcgtagattc agccaaaact ggtcgcatca gggctttccg
      721 gcgagccgtc cgaatcgtaa acggctggga tattccgcga tggtacctct cccaggatgg
      781 tccagtccca cgccattttt caggaatgtc gcttatttgt gaaaacccgg tttatatcta
      841 tggcaactta aactcaattg gtgttcagga acagccatca acaattgttg accctgattc
      901 gggtctgcca gttggtggac caactgcctc ccgacggttc ctccaaaccg gaaaccgaaa
      961 catgccattg gtgccaggga actttaactc aaatcctcca gtgactggcg gcgctgacga
     1021 tgccctcctc cacgggccaa tgtcaattat ggctgatgcg gtctcgatcc attcacgagc
     1081 gttccaggat tcgagaatgt ttatttgttc tcgtgggtgg ggtttcaggc cacaaaaccg
     1141 attgcggcga tacgcgatgg aaaccacggt taaggccgcc tggttgaccg gacagccgaa
     1201 agcaggtgtt caaactacat cacaacaaac cagtgatgat aacggagat
//
LOCUS       DAOQDO010002259         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_139821_length_1248_cov_2.538139, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002259 DAOQDO010000000
VERSION     DAOQDO010002259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139821_length_1248_cov_2.538139"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PKZ53_26485"
     CDS             complement(<1..1230)
                     /locus_tag="PKZ53_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44059.1"
                     /translation="MLSNGFSAEFGRNSGAVVNVATKSGSNDFHGTLFEFLRNNVFDA
                     RNFFENAGNAPDSKFILNQFGGVLGGPIKRDKLFFFLSYEGIRSRVGNTIFTNVPTLE
                     QRTAVNTGVLDGVRIDVDPVAARLLQLIPLPNANSSFGNFISNNPIRNRNDNGLVRVD
                     YIIGPHDTINARYLINDLSIFTPVQSSSGTSSGAAQVPGYGLKTTVRTQNFTLGSTHL
                     FSSSTVNEARFGLNRVFDFYEGEDRTNPAPLGLPTNPLDSRNVALPQIVISGLSGIGN
                     SNIYPFGDGLTTAQFLDTLTLSRGNHTLKLGADVRLAYVNGFQDFSFSGTVSFDGAVS
                     GISSLYDFLQGTPSPQTTFITRGLTAPPIRQQNYYFFVQDDWKVRTGLTFNLGLRYEL
                     NTVPTASGRLTNFTPERG"
BASE COUNT          330 a          314 c          315 g          289 t
ORIGIN      
        1 cccccgctct ggcgtgaaat tggtcaatcg gccactggcg gttggaacgg tgttgagttc
       61 atagcgcaat ccaaggttga aagtcagccc ggtgcgaacc ttccagtcat cctggacaaa
      121 gaaatagtag ttttgctgcc tgattggcgg tgccgttagc ccacgagtaa tgaaggtggt
      181 ctgaggtgat ggcgtgccct gcaaaaaatc atagagtgaa ctgataccgc tcacggctcc
      241 atcaaaactg accgttcctg aaaagctaaa gtcctgaaaa ccattgacat aagccagtcg
      301 cacatcagcc cccagcttga gcgtatggtt tccgcgcgag agcgtgaggg tgtcgagaaa
      361 ctgggctgtt gttaacccat cgccaaatgg ataaatattg ctgttgccaa ttccagataa
      421 cccggagatg acaatttgcg gaagagcaac gttgcggctg tcaagtggat tggtcggcaa
      481 cccgagcggc gccgggttgg tccggtcttc accttcataa aagtcaaaca cgcggttgag
      541 accaaaccgg gcttcgttga cggtagatga agaaaacaag tgggtacttc cgagcgtgaa
      601 gttttgggtg cgaacggttg ttttcaaccc atatcctggc acctgcgccg ccccgctcga
      661 tgtgcccgaa gatgattgaa ctggcgtaaa tatgctcaga tcattgatca gatagcgggc
      721 attgattgta tcgtgcggcc ctatgatgta atccacccgc accaatccat tgtcatttcg
      781 gttgcgaatt ggattgtttg aaatgaaatt tccaaagctt gaattcgcat tcggaagcgg
      841 aatgagttgg agcagtcggg ccgctaccgg atcaacatca attcgcactc catccagaac
      901 cccagtattg accgccgtgc gctgctcaag ggttggaaca ttggtgaaaa tcgtgttccc
      961 cacccgtgac cggatacctt cataactcaa gaagaaaaac aacttgtcac gtttgatcgg
     1021 accgcccagc acgccgccaa actggttcaa aatgaacttt gaatctggcg cattgccagc
     1081 attttcaaag aagttacggg cgtcaaacac gttattgcgt agaaattcaa aaagtgtccc
     1141 gtgaaaatca tttgaccccg atttggtcgc cacattcacc acggcacccg agttacgccc
     1201 aaattcagca ctaaaaccat tagaaagcac ctgaaattct tgaacggc
//
LOCUS       DAOQDO010002260         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_140014_length_1247_cov_3.298658, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002260 DAOQDO010000000
VERSION     DAOQDO010002260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140014_length_1247_cov_3.298658"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..930
                     /locus_tag="PKZ53_26490"
     CDS             553..930
                     /locus_tag="PKZ53_26490"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ44060.1"
                     /translation="MLPTDRLSIVAFDNRVEIVCPYQPVTDKAEIKSKISRITAGGNT
                     GLHEAGVRGAMEVNRFSEVFTHNRILLVMDGQANVGETNPATLIGQAREMVVHRLSTS
                     TIGIGNDFNEELLRSQNSKVIGA"
     gene            984..1118
                     /locus_tag="PKZ53_26495"
     CDS             984..1118
                     /locus_tag="PKZ53_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44061.1"
                     /translation="MKWEMLSDPEVERRLANLEIKSFATRDTQEVAGYTEVMDTIFQA
                     "
BASE COUNT          344 a          260 c          301 g          342 t
ORIGIN      
        1 atataactct tcaaattttt ctccccagca gtcactcaaa cctggacatt cattgtcgtt
       61 ggtgtcaaaa agtgttattt gcggagaaag gttcacttcg gtgaagaaga agttacggaa
      121 gcacactgaa ctcccgataa gtagaacctt gaacgcccgt gttatctgga accaaaccaa
      181 actccatatt gagattggct acctggagta ttggggatat tggggattag gggctatgtc
      241 ccttcaaaac caatgcttgt tagatctggt aattaatact atattgatat cagttttata
      301 ctaaaataca cttagttaag tactaaatag gtatatatat tgtacttata tagtatcaat
      361 tcagtaccag tttaaaacta caacctgtat actctcaatc acacagttgt tcttatactg
      421 gctctccaca tacacaaata tatttctcta tttcttaaat actgttgata gtattcactg
      481 gcataactat ttttcttcca tgaacccact cttgctggtg gtcaaacgac tccaggctga
      541 attgtagatc agttgttacc cactgacagg ctgagtattg tcgcgtttga taatcgggtt
      601 gagattgtct gtccgtacca accggtgacg gacaaagctg aaatcaaatc aaagatctca
      661 cgcattacgg ctggtgggaa taccgggctg cacgaagcag gggtgcgtgg cgcaatggag
      721 gtgaaccgtt tttcagaggt gtttacccat aaccggattt tattggtgat ggatggtcag
      781 gccaatgttg gcgaaaccaa tccggcgacc ctgatcgggc aagcacggga aatggtagtg
      841 catcggctta gtacctccac gattgggatt ggaaacgatt ttaacgaaga actgctgaga
      901 tcgcagaatt caaaggtgat tggcgcgtga ttggtagcct ggcacccgtg cgccggtggc
      961 taccatcgaa ggtatcaggt cgcatgaagt gggaaatgtt atcggatcct gaggttgaac
     1021 gccggcttgc caaccttgaa atcaaatcct ttgccacgcg tgacacgcag gaggtggctg
     1081 gatataccga ggtcatggat accattttcc aggcatgaga ggtcatcaaa atcaccgaga
     1141 accatattcg gtatgacaac gattcatttt tatgccgggc ttgggataaa gcggacttcg
     1201 agggctggcg gaatgaaaac ctggctggcc aggatgagca cctttgt
//
LOCUS       DAOQDO010002261         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_140087_length_1247_cov_2.250839, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002261 DAOQDO010000000
VERSION     DAOQDO010002261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140087_length_1247_cov_2.250839"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /gene="rpsT"
                     /locus_tag="PKZ53_26500"
     CDS             complement(<1..215)
                     /gene="rpsT"
                     /locus_tag="PKZ53_26500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNJ44062.1"
                     /translation="MPNHESAIKRDRQNKRRNEVNRKNRARVRTFIKKLRSLLSVKTK
                     DEAIAQLPPTISAIDKAVQKGVIHQNAA"
     gene            506..856
                     /locus_tag="PKZ53_26505"
     CDS             506..856
                     /locus_tag="PKZ53_26505"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNJ44063.1"
                     /translation="MGEWIKVAERRQVRQGSFFVVEVKEKKIILYNIAGEYFASARGC
                     PHMGAPLDEGACAGTEVICVWHHWSFDLRTGKCTSNALSPADLRLYPVKLEGGALWLE
                     YSDDPPASDTLPPG"
     gene            complement(881..>1247)
                     /locus_tag="PKZ53_26510"
     CDS             complement(881..>1247)
                     /locus_tag="PKZ53_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44064.1"
                     /translation="DLAGLASMETARLRLDRTYTPSNLQHIAQTRQVRIAVLYEKWFV
                     GKIPSQWIKVGEWQIPNNIICGDDRVAFFAVVPDECHVLRNNLIEFSAQLPPGVKASL
                     TNLPSDLQPSSSDQPSPAR"
BASE COUNT          297 a          274 c          339 g          337 t
ORIGIN      
        1 gcagcgttct ggtgaatcac acctttttga actgccttgt caatcgcaga gatggttggt
       61 ggcaattgag caatagcttc atctttggtt ttgacactca acaagctgcg cagcttcttg
      121 atgaaggtgc gaacccgggc gcggttttta cggttgactt cgttgcgacg tttgttttga
      181 cgatcacgtt taattgcaga ctcgtgattt ggcatgaacg actccctgta agatcagtct
      241 tccttgagac aaaagttaat ggtgtgtgat tgggataaag cgaatgacgc aaaccgcgta
      301 atttacggat gaaggcggcg gctgtcaaga tattcggcga ggatttttca gcgcggtttg
      361 gaattgaggg gaggaagtgc cggtccgtag ggatgatttg caccattctg gattcctgaa
      421 tagagtacca ccggtcttat gatcggctca ttcccttctg gtcagatttt caatttttca
      481 attttagtca ggtagaggtg tttccatggg tgaatggatc aaagtcgccg aacggcgtca
      541 ggttcgtcag ggaagctttt ttgtcgttga agtcaaagag aaaaaaatca ttctatacaa
      601 tattgcgggc gaatattttg cttcagcccg aggttgccca catatgggtg ctccgctcga
      661 cgaaggcgcc tgtgccggaa ccgaggtcat ttgtgtctgg catcactggt cctttgatct
      721 gcggaccgga aaatgtacca gtaacgcgct gtcaccagcc gacctcaggc tgtacccggt
      781 caaattagaa ggaggggcct tgtggcttga atattcagac gacccccctg cctctgatac
      841 tcttccacct ggataatgca aatggctacg ggaagaacga tcagcgggca ggcgatggtt
      901 gatctgaact tgatggttgg aggtcagaag gcagattcgt caatgacgcc ttgactccgg
      961 gtggaagttg agcggagaac tcgatgagat tgtttctcag cacatgacat tcatctggga
     1021 ccacggcaaa aaatgccacc cggtcatctc cgcaaataat attgttggga atctgccact
     1081 ccccgacttt gatccattgg gagggaattt tcccaacaaa ccatttttca taaagaactg
     1141 caattcggac ctgtcgggtt tgtgcaatgt gttgcagatt tgaaggggta taggtgcgat
     1201 caagccgaag tcgagctgtc tccatgctgg ctaaccctgc cagatca
//
LOCUS       DAOQDO010002262         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_140457_length_1246_cov_1.313182, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002262 DAOQDO010000000
VERSION     DAOQDO010002262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140457_length_1246_cov_1.313182"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PKZ53_26515"
     CDS             complement(<1..1216)
                     /locus_tag="PKZ53_26515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44065.1"
                     /translation="MEAISEQASASLDRSSDPSDYRLLIEYIRRAVRFQHRVLAPDQE
                     EILVRLVSGFGVPEFKLSSELCKASFYSCLPVWADAHSQMFEKRGDFNGQGQMINREL
                     SYRFRINGIDPETQKTAFLSRLARIRERLAVQTDGAVSPRTLAELIFLWNVIPTDEAH
                     PAHPDWTAPLLFLEQGGPTAAQELLKSLESRAEIVSRIGRTFVQVLKQSGGCVPESLQ
                     RRPVVFYLSLRKSLVNWVKAFTEGTLDPLIGQGTDAMVKAEWLRHVDIYDAPNPSGLL
                     SIQVSARLSEHRLAVSGESFHIPAYREYPAGVVQILWHPTELKEVKSEHWAFPSRIEI
                     RYEDKYLGSKNRNLDSDQEKNLAIYRLSFAILVETVLSTILRSVKNAIPDTFDISVMM
                     FRIQTGGIEAEPH"
BASE COUNT          325 a          339 c          285 g          297 t
ORIGIN      
        1 tatggggttc agcctctatc ccgccggttt gaatccggaa catcatgact gaaatgtcga
       61 aggtgtcggg aatggcgttc ttcacagatc gtaaaattgt ggagagaacc gtttcgacca
      121 ggatcgcaaa actgagtcgg tagatcgcca ggttcttttc ttgatcagaa tctaaatttc
      181 tattcttgga acccaggtac ttatcctcat atctgatctc aatccgactc ggaaatgccc
      241 aatgctcaga cttgacctcc tttaactctg ttggatgcca aagaatttga accaccccag
      301 ccggatattc acgatatgcg gggatgtgaa aagattcacc tgaaacggcc agtcggtgct
      361 cagaaagccg ggcactcacc tgaatcgaaa gcagtccgct gggattgggg gcatcataga
      421 tatcaacgtg gcgcagccat tcggccttga ccattgcatc agtcccctga ccaatcagcg
      481 gatccaaagt gccttcggta aatgccttca cccaattgac aaggcttttc cgaagcgaca
      541 ggtagaacac cactggtcgc cgttgtaatg attctggaac acaacctccc gattgtttca
      601 gcacctgaac aaaggttcgg ccaatccggc ttacaatttc agctctggac tcaagggatt
      661 tgagtaattc ttgagctgcc gttggccccc cctgttccag aaaaagcaag ggcgccgtcc
      721 aatctgggtg agctgggtga gcttcatcag tcggaatcac attccataaa aatatcaatt
      781 ccgccagggt tcgagggctc actgccccat cggtctggac cgccaaccgc tcccgaatcc
      841 tggccaaccg ggacagaaac gcggttttct gggtctccgg atcgataccg ttgatgcgaa
      901 accgatagct gagttcgcga ttaatcatct gcccctggcc attaaaatca ccccgtttct
      961 caaacatttg tgaatgagca tctgcccaca caggcaaaca actgtaaaaa gatgccttac
     1021 acagttctga actcaattta aattctggca ctccaaaccc agagaccaat cgaaccagaa
     1081 tttcttcctg gtcaggggcc aaaactcgat gctgaaaccg taccgcccgg cgaatatact
     1141 caatcaatag ccgataatca gagggatctg agcttctgtc caggctggct gaagcctgtt
     1201 ctgaaattgc ttccatgatc ttgaaggcaa ccgccagcct gactct
//
LOCUS       DAOQDO010002263         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_140490_length_1245_cov_3.185714, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002263 DAOQDO010000000
VERSION     DAOQDO010002263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140490_length_1245_cov_3.185714"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..386
                     /locus_tag="PKZ53_26520"
     CDS             168..386
                     /locus_tag="PKZ53_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ44066.1"
                     /translation="MTNQPRYEMIIYWSENDQAFLVEVPELPGCMADGATYTEAVTNA
                     EVIIREWIETARELGRPIPEPKGRLIYA"
     gene            complement(462..>1245)
                     /locus_tag="PKZ53_26525"
     CDS             complement(462..>1245)
                     /locus_tag="PKZ53_26525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ44067.1"
                     /translation="LAAFLLILFGGVTDRLIPLFAVGAFLAFTLSQAGMVVHWWRLKG
                     KSAWLNMTINGAGALATGVTLFVVLIAKFTEGAWVTLFLIPAMLSVFAAVRRHYHSVA
                     LEVSCPRPLDVSDLRDPVVIVPIREWNKMAHKALRFALKLSPDIFGVQIRAAERMDDL
                     NSQWKQFVEDPVSRAGLPVPHLVVLESPYRRTLNPLVDYVLEMRDRYPDRQIAVLIPE
                     LVEFRWYHYLLHNQRAQVLKALLLLKGGQRVIVIAVPWHLSA"
BASE COUNT          329 a          351 c          302 g          263 t
ORIGIN      
        1 gtccggatga tttcactgat gtaaatctct gtagatcaat agtttcacta tttcattgaa
       61 aatttgtggc atccttgcac tggctttaca gcacacaata gacaagctga agactgaact
      121 ctgaactttt ctgcattttg ggatcctgac cccggaagtg agccagaatg accaatcaac
      181 cccgttacga aatgatcatt tattggagtg agaatgatca ggcattcctg gtcgaagtcc
      241 cggaacttcc tggctgtatg gctgatgggg cgacctacac cgaggctgta accaatgctg
      301 aagtgatcat ccgcgaatgg attgaaactg cgagagagct tgggcgtccg attccggaac
      361 caaaggggcg gttgatctac gcctgattga taaaccaact gtgagttgac ccccctcttg
      421 atgtcattgc gctttcaaat caacgcattt gggttattcc ctcacgctga aaggtgccac
      481 ggcacggcga tgacgattac ccgctggccg cccttgagca acaacaacgc tttcagtacc
      541 tgggcgcgct ggttgtgcaa taaataatga taccagcgaa attcgactaa ctccggaatc
      601 aacaccgcaa tctgccggtc aggataccga tcccgcattt ccaaaacata atccaccagc
      661 gggttgagcg tgcgccggta tggcgattcc aacacaacca ggtgcggcac cggcaacccg
      721 gcgcggctta ccggatcttc aacaaactgt ttccactgac tgttcaggtc gtccatccgt
      781 tcggctgccc gaatctgcac cccgaaaatg tcaggcgaga gctttaaggc aaagcggagc
      841 gccttgtggg ccatcttgtt ccactcccga atcggaacaa tcaccaccgg gtcacgcaaa
      901 tcagacacat ccagcggtcg agggcaactt acttcaagcg ctacggaatg gtaatgccga
      961 cgcacggcgg caaataccga cagcatggct ggaatcagaa ataacgtcac ccaggcgcct
     1021 tctgtaaact tggcaatcaa caccacaaac agcgtcactc cggtcgccag agcgcccgct
     1081 ccattgatgg tcatattcaa ccaggcggac ttccctttca gtcgccacca gtgaacgacc
     1141 attcctgcct gcgaaagggt aaaagcgaga aacgcaccga ctgcaaaaag tggaatcagc
     1201 cgatcagtca cgcctccgaa cagaatcagc aggaaagctg ccagc
//
LOCUS       DAOQDO010002264         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_140768_length_1244_cov_2.518082, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002264 DAOQDO010000000
VERSION     DAOQDO010002264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140768_length_1244_cov_2.518082"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>1244
                     /locus_tag="PKZ53_26530"
     CDS             32..>1244
                     /locus_tag="PKZ53_26530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ44068.1"
                     /translation="MTSQNFIAPGFEAVQTVFEEHFTRGWDVGSAFAVFVDGQVVVNL
                     AGGIRSKTADAPVAYDTDTIQLLVSCSKFVESLCIAVLVDRGWLAYDDRMVDHWPEFA
                     PEGSTKAQITVRQLMMHRAGLPVFDRNLTDDELFNHDQRELFLEQQSQVAELFEPEPA
                     DGNWRQQSPAPPHAYHAVSRGLYSGALLRRIDPQHRTMGQFLHEELALPLGIDIWLGL
                     PEAEEHRVSPTTADRVGVMHVTGLQPSPEIAPDDPRYQVFEYEVDFFKQFLTQPESLV
                     SRCLNTVSLKDVFPQELGTHRSMRACEMASSTCVGNALALAKLAALVVGGGEIEGKHI
                     FAHPETLDQLAELAESYARDSILGTPVEYTQGGLARFFAQDSQQTLNYGWGGAGGLIV
                     RYVPSLKLGCAY"
BASE COUNT          268 a          313 c          347 g          316 t
ORIGIN      
        1 attgataccc tgcaatgttg gaggctcccc cttgaccagt cagaatttta tcgcacctgg
       61 gtttgaagcg gttcagacgg tgtttgaaga acattttacc cgtggttggg acgttggatc
      121 ggctttcgct gtctttgttg atggccaagt ggtcgtcaat ctcgccggag ggattcggag
      181 taaaaccgct gatgcgcctg tggcctatga caccgacaca attcagttgc tggtgtcgtg
      241 ttcaaaattt gtggaatcac tgtgtattgc cgtgctggtg gatcgtgggt ggcttgccta
      301 tgatgaccgg atggtagatc actggccgga gttcgccccg gaaggttcaa ccaaagctca
      361 aattacggtg cgccagttga tgatgcaccg ggccgggttg ccggtttttg atcgaaatct
      421 caccgatgac gaactcttta atcacgatca acgcgaactt tttcttgaac aacaatccca
      481 ggtggccgaa ctttttgaac ctgaacccgc ggacgggaat tggcgccaac aatcgccagc
      541 gccaccccat gcctatcacg ctgtatcacg cggcctgtat tctggggcgc tattgcgtcg
      601 catagaccca cagcaccgaa ccatgggcca gtttctacac gaagaactgg cactcccgct
      661 gggaattgat atctggcttg gactcccaga agctgaagaa catcgggtgt caccgacgac
      721 agcggaccgc gttggagtga tgcacgtcac tggactccag cccagtcccg agattgctcc
      781 tgatgacccg cgctatcagg tttttgagta cgaagtggac ttttttaaac aatttctgac
      841 ccaaccggag tcgctggtgt ctcgatgttt gaacaccgta tccctcaaag atgtttttcc
      901 gcaagaactt ggcacccacc gaagtatgcg agcctgtgag atggcttctt caacctgtgt
      961 tggaaatgcg ctggcactgg caaaactggc ggccctggtt gtcggaggcg gtgaaattga
     1021 ggggaaacac atttttgcgc acccggaaac gcttgatcaa cttgctgaac tggccgaaag
     1081 ctatgccaga gattctattt tagggactcc ggtggaatac actcagggtg gtttggcccg
     1141 cttttttgcc caggatagtc agcaaacgct caattatggg tggggcggtg caggcgggtt
     1201 gattgtgcgg tatgtccctt cgctcaaact tggttgtgcc tatg
//
LOCUS       DAOQDO010002265         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_140954_length_1243_cov_3.205387, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002265 DAOQDO010000000
VERSION     DAOQDO010002265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140954_length_1243_cov_3.205387"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            640..>1243
                     /locus_tag="PKZ53_26535"
     CDS             640..>1243
                     /locus_tag="PKZ53_26535"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44069.1"
                     /translation="MSIDPWPTRVAAQTDLRGQAQRAFADGEVERRKGTQESLIKSIE
                     LYQEALSAYRKLKDQRGEALSISTIGLVFARLGASQQAIDAYTQALELCRTLKDKPGE
                     ARNLIALSDAYDDIGEKQKAIEAQAAALKLWREQQNQFGEATSLTSIGLLYARVDQPK
                     TALDFLTLALASWRAQQNRQGEARTLSYLAGISHASGRRRL"
BASE COUNT          301 a          323 c          337 g          282 t
ORIGIN      
        1 ggtattgggt aaccattttg tgtgggtcga gggccaggca gggcaacccg cacccaatgg
       61 ccagccagac acaaaaaaag aggaaccgta ataccatagg gagttgaaaa cctgagttga
      121 aaaaagaagc tgcgggaaaa tatgctcagc aaggatgcac gtgagagtgt tgccactgaa
      181 ggggcatcct tcctgggagt ggcctttcag gtggtgtgca atcagtgtgc cggaaccgtt
      241 atgcgagcag tgtgaaacgt aggttgtgag tcagttgctc gtcaagttgc tttttctgag
      301 cggtttaact gctgtttttc cacaaaggta attccatcag aagcagtgca aacggggaat
      361 tctcaccctg gactctggca tttttcagcc cacccaggtt cggctttctg gtcctggaac
      421 ccgaaacctg gaatcaggcg ttcggaatct ggaaacgtga atcccaaatt ggtttgagaa
      481 acctgagcaa aactggctac actgggcccg ttttatccaa cctccgaacc atccaacgct
      541 ctattccaat cacatctcat tccgactttt ttgaaaatca tggtgtatcg tttcttgctt
      601 gttcttgtgg ccctgggtgt cctgggggga tccactgttg tgagtattga cccatggccc
      661 accagggttg ccgcccaaac tgatcttcgc gggcaggccc agcgtgcctt tgctgatggt
      721 gaagtcgagc gccgaaaagg cactcaggaa tccctgatca aatcaattga actgtatcag
      781 gaggcccttt ctgcctaccg caaactcaaa gaccagcgtg gcgaagccct ctccatttca
      841 acgattggcc tggtgtttgc ccgtctgggc gccagccagc aggccattga tgcctatacc
      901 caggcgttgg aactgtgccg aacactcaaa gataaacccg gcgaagcccg caatctgatt
      961 gcactcagcg atgcctatga cgacattggc gagaaacaaa aagcgattga agcacaggcg
     1021 gcagccctca aactctggcg tgaacagcaa aaccagtttg gcgaggccac ttcgctgacc
     1081 agtatcggat tgctctatgc ccgggtagat cagccaaaaa ctgcgctcga ttttcttacc
     1141 ctggcgctgg caagctggcg ggcgcaacaa aaccgtcagg gagaagcgag aaccttgagt
     1201 tatctggcgg gcatttcgca tgcgtctggg cgccggcgac tgt
//
LOCUS       DAOQDO010002266         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_141020_length_1243_cov_2.351852, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002266 DAOQDO010000000
VERSION     DAOQDO010002266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141020_length_1243_cov_2.351852"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..>1243
                     /locus_tag="PKZ53_26540"
     CDS             591..>1243
                     /locus_tag="PKZ53_26540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ44070.1"
                     /translation="MNLHDLKKLVEGTADAAFVSDSNGTIIGWNKGAEALFGISAAEA
                     VGKFCGDMVSGHDECGQVCSQECSVQQAVRENRPMSNFDLHIKSQQGYQWTNTSTLLV
                     SVANSEFPYSVHIFRRIDVRKRLELLIRDFIVAETQLPAEQVTSLISTTRAPAAAIEL
                     TDRELQVLKLMARGLPTSQIATTLHISRTTANNHIQHILQKFNVHSRLEAIRRAELAG
                     "
BASE COUNT          340 a          313 c          263 g          327 t
ORIGIN      
        1 gctgagggta tcgggctaag ggctgaagaa aatacaatat tttcaacatt ttagcttttc
       61 cataatgcga gcacttcatt ccgaaatggt ataagaccat ctgaggctga gggaattaca
      121 atcttttcaa cgattggcct tccccatatc accaggggtt cattccaaaa aggtacagtg
      181 tcaaggattt tgaaacttca gagttgcaaa caaaaaaagg gggttggcgc tcgccaaccc
      241 ctaaattgca ttgtcacctt ttgccgactt acttgcacaa gaagaaaatg agaattatcg
      301 cacgatcaat ttcacacaca ttacaagagc ctggaaatgt atttatcgaa gtcttcgata
      361 ccataatttc tccctcgtat tgagtgccgg agttttacct tccctctcat taaaaagcct
      421 caagaaaaca tcattttttt ctcaaaatag gaatttcatt tcacttcaaa gccgggtaag
      481 atgatttcct gcagcttcta acttctctta aacaaaccag ataccagacc aggaagtaat
      541 tgttccaaat tgctgttcca ggcgctcaaa acgacagaat cgagggtttt atgaacctgc
      601 atgacctcaa aaaactggtt gaaggcaccg ctgatgctgc gtttgtttca gattcaaatg
      661 gaacaatcat tggatggaat aaaggcgccg aggcattgtt tgggatttct gcggctgaag
      721 ccgtcggaaa attttgtgga gatatggtca gcggccatga tgaatgtggc caggtttgct
      781 cacaggaatg cagtgtccag caggcagtgc gtgaaaaccg ccccatgtcg aattttgacc
      841 ttcatatcaa atcccaacag ggctatcagt ggacgaatac ttcgaccttg ctggtctcgg
      901 tcgccaactc tgagtttcca tattcagttc atatttttcg ccggattgat gtccgcaagc
      961 gattggaatt attgatccgc gattttattg tggccgaaac ccagcttccc gccgaacagg
     1021 ttacttccct gatttccacc acccgcgccc cagccgccgc cattgaactg accgaccgtg
     1081 aattgcaggt ccttaaattg atggctcgcg gccttcccac cagtcagatt gccaccacgc
     1141 tccacatcag ccgcaccacc gccaacaacc acatccagca tatccttcag aaattcaatg
     1201 tccactcgcg ccttgaagcc attcgtcggg cagaactggc tgg
//
LOCUS       DAOQDO010002267         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_141882_length_1239_cov_2.864020, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002267 DAOQDO010000000
VERSION     DAOQDO010002267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141882_length_1239_cov_2.864020"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKZ53_26545"
     CDS             <1..463
                     /locus_tag="PKZ53_26545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ44071.1"
                     /translation="IVGTPAYMAPERLAAHPYDGRSDIYSLAVMMYELLCGRQPFAEH
                     AQAFASLVVAHLQYPPPPVRKFAEYVPEAVEALVLCGLSKKPVDRPTAQEFADQIAQI
                     LNILPEESDQLPHSARSADQNREIAALPTAEFTTTNLVETQNLPSTRTNPE"
     gene            606..>1239
                     /locus_tag="PKZ53_26550"
     CDS             606..>1239
                     /locus_tag="PKZ53_26550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ44072.1"
                     /translation="MLEPSPPVEDLLLPHVLLISTAPDPLSDILSEFGFSFSLTDPSL
                     GREFTQIPDIVVTQHIESSLLTQLSTRFHLIPIVGVVTTQAEDTAFEAFEQGQLQDYV
                     LLESPKRLKPCLTSLHREYFDRRLRRLVNEVPEAMTVVSSDGSTRYTSQNNVYVLGRN
                     LAEVHGINGFTYIHPDDVALLQSEFDQLVTHPETIKTALVRIQHKDGTWRW"
BASE COUNT          300 a          332 c          284 g          323 t
ORIGIN      
        1 gattgtggga actccggcct atatggcgcc ggaacgtctg gcggctcacc cgtatgatgg
       61 ccgctctgat atctacagtc tggctgtgat gatgtatgaa ctcctgtgcg gcaggcagcc
      121 gtttgccgaa cacgcccagg catttgccag tctcgtggtg gctcacttgc aatatccccc
      181 gccaccagtc cggaaatttg ctgaatatgt tcctgaagcc gttgaagcac tggtcttgtg
      241 tgggttatcc aagaaaccag ttgatcgccc aactgcccag gaatttgcgg accagattgc
      301 ccagattctc aatatactcc ctgaagaaag tgatcaactt ccgcactcag cccgatcagc
      361 ggaccagaat cgggagattg cggccttgcc cacggctgag tttaccacca ccaatctggt
      421 ggaaacccaa aatttgccat caactcgaac caatcctgaa taactccgct cgaaattccg
      481 ctataattga gacgctgacc tctgaggtgc cgcaaaatga ttgttgcttc ttattcgttc
      541 ctgatatgtt gagaacgtca atcaacagaa aagtttagcc agtcaatttc ctgccaggta
      601 cctttatgct tgagccgtca cctcccgttg aggatttact cttaccgcat gttctcttga
      661 tttcaactgc ccccgatcca ctgtctgaca tcctgagcga gtttggattc tcattcagtc
      721 tcacagatcc atctcttgga agagaattca cccagatccc agatattgtg gtgacgcaac
      781 acattgagtc atcgctgtta acccagctca gtacccggtt tcatctgatc ccaatcgttg
      841 gtgtggttac aactcaggca gaagacactg cctttgaggc atttgaacaa ggccagctcc
      901 aggattatgt cttgttggaa tcacccaaac gactcaagcc gtgccttacc agccttcatc
      961 gagagtattt tgaccgtcgg ctccggcgac tggtcaacga agttccagaa gccatgacgg
     1021 tggtttcttc tgatggatca acccgatata ccagtcaaaa taatgtctat gttctggggc
     1081 ggaatttagc tgaagtccat ggaatcaatg ggtttacgta tattcacccg gatgatgttg
     1141 cccttttaca gtcagaattt gatcaactgg tgacgcatcc ggaaaccatc aaaacggccc
     1201 tcgttcgaat tcagcataaa gatggcacct ggcgctgga
//
LOCUS       DAOQDO010002268         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_141927_length_1239_cov_2.360642, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002268 DAOQDO010000000
VERSION     DAOQDO010002268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141927_length_1239_cov_2.360642"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PKZ53_26555"
     CDS             complement(<1..709)
                     /locus_tag="PKZ53_26555"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ44073.1"
                     /translation="MSTLLFHKTFFRLLFGIIGLAMMLCCSLEVVAQTVVINSVNPNQ
                     VSVGQVPAKVIIKGTGFTPQSTVFFNDVQVAAKIKKSSKKIVVTQLPTELFQVVGAIQ
                     VRVIPPSGAPSNSFMLAVGSLNRIQVSQPNGFSLAVGEQYSIQAQVLDQSGNPVPNAQ
                     IGYTSLNPDKATVDATGVVRGLANGVGTIRVTSGDAFLNLIFTVTEVASIPSGIVGDG
                     DLKVDANNQVYATDLRNH"
     gene            1031..>1239
                     /locus_tag="PKZ53_26560"
     CDS             1031..>1239
                     /locus_tag="PKZ53_26560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44074.1"
                     /translation="MKKIKNWLLGALSLSVCCLLGISLNTFRLNHFSPHWNNARLTMS
                     GRQIQILPAGEKFLIPSSWVDWHNK"
BASE COUNT          372 a          286 c          261 g          320 t
ORIGIN      
        1 catgattacg caaatcagtg gcatatacct gattattggc atctaccttg agatcaccat
       61 cacccacaat tcccgacgga atagacgcca cttcggtcac cgtaaaaatc aaattgagaa
      121 acgcatcgcc ggatgtaact cgaatggtac caacaccatt tgccaggccg cgaaccacac
      181 cagttgcatc aacggtggct ttgtctggat ttaaactggt gtagccaatt tgggcattgg
      241 gaaccgggtt gccagactgg tcaaggacct gagcctgaat tgaatactgt tcaccaaccg
      301 ccagagaaaa cccattgggc tggctgacct ggattcgatt gagactccca actgccagca
      361 taaaactatt tgacggggca ccgctgggtg ggatgactct cacctgaatc gccccaacga
      421 cctgaaacag ttcagttgga agctgggtca caacaatttt tttactcgac ttcttaatct
      481 tcgcagccac ctgaacatca ttgaaaaaga cagttgactg cggtgtaaac ccggttcctt
      541 tgataataac tttcgctggg acctggccaa cactcacttg atttgggttc accgagttga
      601 tcacgacggt ttgcgccacg acttcaagcg aacaacacaa catcattgct aaaccaatga
      661 tcccaaacaa caaccggaag aaggttttat gaaataacaa ggtactcatg cgatcaccag
      721 gcgatacaga aagggatgat tttggctgaa aggaaaaaca gggtgatttt gaagcatatc
      781 aaaaccaaga taagcagagt ataaattagg acgaccgtgt tgaattatgg aataaccggt
      841 ttctttccag gaaaatcccg attttgaaag ttactgaaca tcagaatcag tggctggatc
      901 aaactaagag gagtgatctt tattctctga caaacctcaa ttttcaacct gttttccttt
      961 tgcaatggaa cttcctgcaa ttgtcagggt ctaacacgtt caaattgcaa tcttttccgg
     1021 gagaattcga atgaaaaaaa taaaaaactg gctactcggc gctttaagtt tgagtgtttg
     1081 ttgtctgctt ggaatatccc tgaatacatt ccgactcaac catttttcac cacactggaa
     1141 taatgccaga ctgacgatgt caggccgtca gatccaaatt ttacctgcag gcgaaaaatt
     1201 tttaattcct tcatcgtggg ttgattggca caataaatt
//
LOCUS       DAOQDO010002269         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_141949_length_1239_cov_2.220439, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002269 DAOQDO010000000
VERSION     DAOQDO010002269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141949_length_1239_cov_2.220439"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>1239
                     /locus_tag="PKZ53_26565"
     CDS             14..>1239
                     /locus_tag="PKZ53_26565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44075.1"
                     /translation="MPQNYQFTHSLAQPQLVNMAQLRAGAAAKKVTAGPKAIQAGTAL
                     SKVTFYGNGATTVLAAADTNDDKVPDGSARETFTAGDPNTEDFAAVAASAKTQKVYAG
                     SFAFDGPTITEGTLTVLDNPNGDFKGNVIREFSIGKGMPAAIAVKDSPAGDIVIVMSV
                     YFQAQSPFDFTDGDFLSITAYLPGADGTPDGSQKIDVLPAGALSLGGDPVVFSFGGMA
                     LSQNGNLIANLVAKNDTEGFAGGATFVFVDNDGDGIPDNQNPPTAFAQFNDANAANLI
                     TAASVVPLEGGKIGVLSADVFSDVEMEIFVYNDANGDGKADDVGTKIFNESRTLPGIL
                     LIYGEGTSGYLVNRMAGSGDKLIFSFIDFQNGPRGSGLASVPVAGGNATKAFDVNNVQ
                     SEIIACVATASGVSSDS"
BASE COUNT          284 a          296 c          342 g          317 t
ORIGIN      
        1 aaaagaagtg gaattgccgc agaattatca gttcacgcat tcgctggctc agcctcagtt
       61 ggtcaatatg gcgcaattgc gtgctggcgc agccgccaaa aaagtgacgg cggggccgaa
      121 agccattcag gctggaacgg cgttgagcaa agtgaccttc tatgggaatg gtgccaccac
      181 cgtccttgcg gcggctgaca ccaacgatga taaagttcca gatggaagcg ctcgcgaaac
      241 atttaccgcc ggtgatccaa acaccgaaga ttttgcggct gtggccgcca gtgccaagac
      301 ccagaaggtc tatgccggtt catttgcctt tgatggacca acaattactg aaggcactct
      361 gaccgtgctc gataacccta atggcgattt caaaggaaat gtgattcggg aattctcgat
      421 tggaaagggc atgccagccg cgattgcagt caaagattca cctgctggtg atatcgtgat
      481 cgtaatgtct gtgtatttcc aggcccaatc gccgtttgat tttaccgatg gcgatttcct
      541 ctcgattacc gcctacctgc caggtgcgga tggcacacca gatggctccc aaaaaataga
      601 tgtgttgccg gctggcgctt tgtcgctcgg tggtgatccg gttgtgttca gttttggtgg
      661 gatggcgctg agccagaatg gaaatttgat tgccaacctg gttgccaaaa atgacaccga
      721 ggggtttgct ggcggcgcta cctttgtttt tgttgataat gatggggatg gcatccctga
      781 taaccaaaac cccccaactg cctttgccca gtttaatgat gcgaatgctg ccaacctgat
      841 taccgccgcg agtgtggtgc cattggaagg cggtaagatt ggtgtattga gcgccgatgt
      901 gttttcggac gttgaaatgg aaatctttgt gtacaacgat gccaatggcg atggcaaagc
      961 agatgacgtc ggcaccaaga tttttaatga atcccggaca ctgcccggca ttctgttgat
     1021 ctatggtgaa ggcacgtctg ggtatctggt caatcgcatg gccggttccg gagacaaact
     1081 gattttctcc tttattgatt tccaaaatgg tccacgtggc tctggtctgg cttcagttcc
     1141 cgttgctgga ggaaatgcca cgaaggcctt tgatgtgaac aacgttcagt ctgaaatcat
     1201 tgcctgtgtg gcaacagcct ctggtgtcag ctctgactc
//
LOCUS       DAOQDO010002270         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_142100_length_1238_cov_3.207946, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002270 DAOQDO010000000
VERSION     DAOQDO010002270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142100_length_1238_cov_3.207946"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKZ53_26570"
     CDS             <1..576
                     /locus_tag="PKZ53_26570"
                     /inference="COORDINATES: protein motif:HMM:NF026953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerY-C metal binding domain-containing protein"
                     /protein_id="HNJ44076.1"
                     /translation="GDDVDIEVLFRITDTVLLMQDFSPINIRKLFSWMTASVTTVSRT
                     IGLGQQNAGLSFAPFSWGQLEMPTRTYSTRSGLPRQVFLHARCSQTGQPYLLRFGRDE
                     QESVYQAVASHRLEIFEDDDESVLPPINSTLLRGCPPCPYCTNPIAAQCVCGALFCDH
                     PDHEITACPTCHLQLKFSGHYVNFNIRQSQG"
     gene            complement(589..>1238)
                     /locus_tag="PKZ53_26575"
     CDS             complement(589..>1238)
                     /locus_tag="PKZ53_26575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44077.1"
                     /translation="MTRFRVPGSGFRVPGSGFSNRFFLPYLSLCFFIRNLDSPKFIMF
                     KSLVFLLVTTLVLGFNSLCVTAHDFHASFTQLEFNPKTKSVEMTLRVFTDDLTNALSR
                     RTGRHIVLDQTPDAESLTFAYVKDRFELRNERGKALRLTWVGMDVKVDMVWIYIEIPM
                     PLWLKQGQLRNEVLTELFADQVNVVGLKANQHKGDLMFAASDRFKPLPELTPIPK"
BASE COUNT          303 a          317 c          315 g          303 t
ORIGIN      
        1 ggagatgacg ttgatatcga agtcctcttt cgcatcacag atacagtgtt gttgatgcag
       61 gatttttctc cgatcaacat ccgcaagctt ttttcctgga tgacagcttc ggtgaccacc
      121 gtgagtcgga cgattgggtt ggggcaacaa aatgccgggc tgtcattcgc acctttttcc
      181 tgggggcaac ttgaaatgcc gacccgaacc tattcaaccc gttcggggct tccgcgtcag
      241 gtctttttgc acgcccggtg tagtcaaacc ggtcagccgt acctgcttcg gtttgggcgc
      301 gatgaacagg agtcagtcta tcaggcagtt gcttcacacc ggctggaaat atttgaagat
      361 gatgacgaat cggtgttgcc tccgatcaat tcaactttgc tgcgcggatg cccaccctgc
      421 ccctattgta ccaacccaat tgcggcccaa tgtgtctgtg gcgcgctctt ttgtgaccat
      481 cccgatcatg aaatcaccgc ctgcccaacc tgccatctcc aactcaagtt ttcaggtcac
      541 tatgtgaatt tcaatatccg ccaatcgcag ggatgattga tgacttcgct attttggaat
      601 tggcgtcagt tccgggagtg gtttaaaccg gtcagaggca gcaaacatca agtccccttt
      661 gtgctgattg gctttcaggc ccacaacgtt gacctgatcg gcaaaaagtt cggtcaacac
      721 ttcattgcga agttgacctt gtttgagcca gaggggcatc gggatttcaa tataaatcca
      781 gaccatgtca acctttacat ccattccaac ccaggtcagg cggagcgctt tgccgcgttc
      841 gttgcggagt tcaaaccgat ctttcacata ggcaaacgtc aagctttcag catctggcgt
      901 ctggtccagg acgatatgcc gtccagtgcg tcggctcaaa gcgttggtga ggtcgtccgt
      961 aaaaacccgg agcgtcattt caaccgattt ggttttgggg ttgaattcga gctgggtaaa
     1021 gctggcgtgg aaatcgtgag ccgtgacaca cagcgaattg aatccaagaa cgagcgttgt
     1081 aaccaggagg aataccagag acttgaacat aatgaatttt ggggaatcca ggttccggat
     1141 aaaaaaacaa agggacaaat agggcaaaaa gaaccgattc gaaaacccgg aacccggaac
     1201 ccggaacccg gaacccggaa cccggaacct tgtcatta
//
LOCUS       DAOQDO010002271         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_142146_length_1238_cov_2.447168, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002271 DAOQDO010000000
VERSION     DAOQDO010002271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142146_length_1238_cov_2.447168"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKZ53_26580"
     CDS             <1..972
                     /locus_tag="PKZ53_26580"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ44078.1"
                     /translation="LFAHAPVSELVSQLESETLRLKTTVNESVTGNLQWGDALVNDVL
                     AEAIRLVEERARFGAVATGLLTGIRRLDEWLNGLNTGLHLLAGSPGVGKTTFALQIAM
                     AVARTVPVVYVTFENSPANLILKAVAGRAGINPQHVQRGWTDISALEKAVQEWRESGQ
                     RVAFIEGTSDLTIAQIRAKTRHAIHRYQATKCLLIIDYLQLWAKMSAEMRGMSSVREK
                     VEVMGGALRELATTLNVPVLALASQNRAGGDYGEGKGKAALDSLKESGDLEYTADVVM
                     FLTASDRRVNKPARAIDLTIAKNRNGEIGKIELIFRPDLSDMREVAS"
BASE COUNT          283 a          301 c          347 g          307 t
ORIGIN      
        1 ctctttgccc atgctccggt ctcagaactg gtcagtcagc ttgaatctga aaccctgcgc
       61 ctcaaaacca cggtgaatga atcagttacc ggaaacctcc agtggggtga tgcgctggta
      121 aacgatgtcc tggctgaggc catccggctt gtagaagaac gtgcccggtt cggagccgtt
      181 gccacgggtc tcctcaccgg gattcggcgg ctggatgaat ggctcaatgg cctcaacacg
      241 ggtctgcatt tgctggcagg ctcgcctggg gttggaaaaa ccacatttgc cctacagatt
      301 gccatggctg tggcacggac ggttccggtt gtgtatgtca cgtttgaaaa ttcaccggca
      361 aatttaatcc tcaaggcggt ggcaggccgg gccgggatta atccgcaaca cgttcagcgt
      421 ggatggaccg atatttcagc gctggagaaa gctgtccagg aatggcgtga atctggccag
      481 cgtgtggcct ttatcgaagg aacttcagat ttgaccatcg cccagattcg ggccaaaact
      541 cgccatgcca tccaccgcta ccaggcaaca aagtgtttgc ttattattga ttacctgcaa
      601 ctgtgggcca aaatgtcggc ggaaatgcga ggcatgtcgt cggttcggga aaaagtcgaa
      661 gtgatgggtg gcgccttgcg tgaactggca accacactca acgtccccgt cctggccctg
      721 gcttcacaaa accgggcggg aggtgattat ggcgaaggaa agggaaaagc cgcactcgat
      781 agtttgaaag agtcaggtga tttggaatac accgccgatg tggtgatgtt tttgacggcg
      841 tctgatcgac gggtcaacaa acctgcccgg gccattgacc tgaccattgc caaaaaccgc
      901 aacggtgaaa ttggaaaaat cgaactcatt ttccgcccgg atttatctga tatgcgcgaa
      961 gttgccagtt aagtttgggg ctgaccaaat ggggttgaga gctgagcttg caaattttcc
     1021 gcccaggaat tggctacttc gctcagtacc tcgtcataag ttttctgagg gattgggttt
     1081 ggtaagtact tgattctttt ctgtggtttc cgtggttaaa atgtttggaa attttcggta
     1141 aggtacttgt ttgctacttc actgcctgag acgttcactc aaacccctgt agccgataca
     1201 ttcgcggtgt cattcccacc tgtttgtgga agacagag
//
LOCUS       DAOQDO010002272         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_142562_length_1236_cov_4.130398, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002272 DAOQDO010000000
VERSION     DAOQDO010002272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142562_length_1236_cov_4.130398"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1127
                     /locus_tag="PKZ53_26585"
     CDS             372..1127
                     /locus_tag="PKZ53_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNJ44079.1"
                     /translation="MIVQRNYSQLTCQACSEGAGLAFDFTMAFQPIVNIRSRSIFGYE
                     ALVRGTNQESAAEILGQVNDDNRYRFDQVCRVKAIQLAARLDLRAFLSINFLPNAVYR
                     PETCIRTTLEAAAKYNFPSDHIIFEVTEGERVTNHQHLIEIFQEYKRLGFKTAIDDFG
                     AGYAGLNLLADFQPDIVKIDMDLTRDIHTHKPRRAIVLGILKVCEELGCQVIAEGIEQ
                     PEELAVLHDAGVHLFQGYLFARPGFEHLPQPQL"
BASE COUNT          327 a          290 c          304 g          315 t
ORIGIN      
        1 tattgtgtta actacccata gttaaaacct tcaaaatttt acgaattcaa agaggaaatt
       61 ctcgattttt tctttgactt cggtgatagc cacgagttcg atgtcgaggt ggtggccatc
      121 accccaaaag ccaaacgtgc caaatatccc cgcatcgttg aaagtaaagg tgagagcttt
      181 cctcaatacc cggatgaaga tgagtgggaa gatgacgaag actgggaatg aagaattgag
      241 agtgaagaat gaagaattga gaaaatggtt agcgggtagt aatcggttcc tggttcttcg
      301 ttcctggttc ttcgtcaaag gtattggttt caagtcaccc gtcacccaac aattaacaac
      361 acatcctctc tatgatcgtg caacgcaact acagccagtt gacttgccag gcttgctctg
      421 aaggtgcagg gctggccttt gacttcacga tggccttcca gccgattgtg aacatccggt
      481 ctcgctcgat ttttggctat gaagccctgg ttcgtggaac aaaccaggaa tcagccgcag
      541 aaatattggg tcaggtcaat gatgacaatc gctaccggtt tgatcaggta tgccgggtca
      601 aagcgattca actggcagcc agacttgatc tccgggcatt tctctccatc aattttctcc
      661 ccaatgcggt ttatcgcccg gaaacctgta tccgcacaac cctcgaagcc gccgcaaaat
      721 acaacttccc ttccgaccat attatctttg aagtcactga aggcgaacgg gttaccaacc
      781 accagcacct gattgaaatt tttcaggaat acaaacggct gggatttaaa accgcgattg
      841 atgattttgg cgctggctat gccgggttaa atctgctggc ggacttccaa cccgacattg
      901 tcaaaattga catggacttg actcgcgata tccacaccca taaaccacga cgcgccattg
      961 tcttgggcat cctcaaggtg tgcgaagagt tgggctgtca ggtgattgcc gaagggattg
     1021 aacaacccga ggaactggct gtcctgcacg atgccggggt gcatttgttt caaggctatc
     1081 tctttgccag accaggattt gagcacctcc cgcaacctca gctttgagga atgaagaatg
     1141 aagaatgaag aatggagaat gaagaatgga gaaaatggtt tttggtgctt ggtgcttggt
     1201 gcttggtgct tggtttcgaa aacccggaac cctgac
//
LOCUS       DAOQDO010002273         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_142590_length_1236_cov_2.906859, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002273 DAOQDO010000000
VERSION     DAOQDO010002273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142590_length_1236_cov_2.906859"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..>1236
                     /locus_tag="PKZ53_26590"
     CDS             375..>1236
                     /locus_tag="PKZ53_26590"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNJ44080.1"
                     /translation="MKRRAFTQLTTAAALAALVPMETRAETQEAKPSATEKVEQDHLE
                     QMKKYEQLVAGPKLTIAMLVYPGMFLQDLVGPLTMFESLLNREIHLVWKTKDLVGNGS
                     TAFPALIPVTPTTTFKDCPAALDVLFVPGGALGTLSMMEDPDVLAFLAEKGKTAKFVT
                     SVCTGSLILGAAGLLKGYRATSYWMTRDVLKVFGAIPTESRVVVDRNRITGGGVTAGL
                     DFGLTLAAKLRNRTYAEAVQLYLEYDPQPPFQAGSPKTANPEAKQFVTDMLSPFAVAA
                     HEAALRAQKRL"
BASE COUNT          305 a          314 c          344 g          273 t
ORIGIN      
        1 ggtactacgc ctcagccacg ccgtttgcca gctttatctt cccaggcgcg ccgtttaccg
       61 tgctggggtc agtcaaaatc acccgccgat aagatcattc agggggcagg ggtttcggaa
      121 gggatgaggg atgagggatg agggatgaaa gaaaaccaat tcttcaactc gttgtcttca
      181 gctctcagcc ctgagttgag ggtttgggac aatcgaagag atgaaagatg aaaaactcac
      241 cttgtcctct tgtcaggatt catggttagg ggaaatacag ttatttcaat ggtttagctt
      301 tccaataacc tgacctggaa tcattccaag atcgcataca ctattttacg agacggcaga
      361 aaagaggaat ttcaatgaag cgccgagctt ttacccaact gacaacggca gccgcactgg
      421 ctgccctggt gccgatggaa accagagccg aaacacaaga ggcaaagcca tctgcgactg
      481 aaaaggttga gcaggaccac cttgagcaaa tgaaaaaata tgagcaactg gtcgccggac
      541 cgaagctcac aattgccatg cttgtctatc cgggaatgtt tttgcaggat ctggtcgggc
      601 cattgacgat gtttgaatcg ctgctcaacc gcgagatcca tctggtttgg aaaaccaaag
      661 acctggtcgg aaatggaagc acggcgtttc cggctctgat tccagtcaca ccgactacga
      721 cgttcaagga ctgcccggcg gcactggatg tgttgtttgt gcccggcgga gccctcggca
      781 cactgagcat gatggaagac cccgacgtcc tggcatttct ggctgaaaaa ggaaaaacgg
      841 ccaaatttgt gaccagtgtc tgcactggct cgttgattct gggggcggct ggattgctca
      901 aggggtatcg ggcgacttca tactggatga cccgcgatgt cttaaaagta tttggggcga
      961 ttccgactga aagccgcgtt gtggttgacc gcaatcggat taccggtggt ggagtgaccg
     1021 ccgggcttga ttttggcctg acgctggcgg ccaaactccg caaccgaacc tatgccgaag
     1081 cagtgcagct ttatctcgaa tatgacccgc aaccgccgtt tcaggctggt tcgccaaaaa
     1141 ccgccaaccc cgaagccaaa caatttgtca ccgatatgct gtcgcccttt gcggttgcgg
     1201 cgcacgaagc agcgctccgg gcgcaaaaac ggcttg
//
LOCUS       DAOQDO010002274         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_142805_length_1235_cov_3.601695, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002274 DAOQDO010000000
VERSION     DAOQDO010002274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142805_length_1235_cov_3.601695"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..618
                     /locus_tag="PKZ53_26595"
     CDS             157..618
                     /locus_tag="PKZ53_26595"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HNJ44081.1"
                     /translation="MEDTSVQLAREPKSEAFSREERILFCRAVANMITADHQVSEEER
                     AYLSGLIRETGLSLLDEEVMLSIESELRSPTPLADLVHPIKSPELRRTLYRAVVEVAL
                     CDRTLAQEEDEYLAKLAELFELNPQAARELIQWTLDSLELEKREADILARL"
     gene            complement(717..1046)
                     /locus_tag="PKZ53_26600"
     CDS             complement(717..1046)
                     /locus_tag="PKZ53_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNJ44082.1"
                     /translation="MQLTLTPKAISEVKKFIADEGLGKEGGLRVRVMPGGCSGFQYAL
                     DVEEAPRDGDNIMDSNGLRVFIDPFSEQYLEGVEIDYVTSVMGSGFTFRNPNAAGSCG
                     CGSSFTV"
BASE COUNT          294 a          298 c          314 g          329 t
ORIGIN      
        1 atgcaggttg tggtactctc ccgaaattca aatcaacagc ttttccactt gttcaaccgt
       61 gttatggctc cctggttacc tgcctcgtat ggagcacctg gctccctcag tatctagtgg
      121 aaactgtgcc aatctttcaa tgcgtgagga ttccccatgg aagacacttc tgttcagctt
      181 gcccgtgaac ccaaaagcga agcttttagc cgtgaagaac ggattttatt ttgtcgggct
      241 gtggccaata tgattacggc tgatcatcag gtttctgagg aagaacgagc ctatctgtcc
      301 ggtttaattc gtgaaactgg tttgtcgtta ctcgatgaag aggtgatgct ttcgattgaa
      361 tcggaactca ggtcgccgac tcccctcgct gacctggttc atcccatcaa aagtccagaa
      421 ctccgccgga cgctctatcg ggccgttgtt gaagttgcct tgtgtgaccg cactctggcc
      481 caggaggaag atgaatatct ggccaaatta gctgaactct ttgaattgaa ccctcaggcc
      541 gcccgagaat tgatccagtg gacacttgac agtttggaac tcgaaaagcg cgaagcagac
      601 atcctggcca gactgtaagt cttattggat aattagtttg ggaaaacaag aaagagcatc
      661 cgggatcata ccctggatgc tctttcgctt ggtcctttgg tggttcgaag gtggaattag
      721 acggtgaagg agctgccgca accgcagctt ccagccgcgt tggggttgcg gaacgtaaat
      781 ccagagccca tgacacttgt cacgtaatca atttccacac cttccagata ttgttcgctg
      841 aatggatcaa taaaaactcg caaaccgttg gagtccataa tgttgtcgcc atcacgtggg
      901 gcttcttcaa catccagagc gtattggaag cctgagcagc ctccgggcat tacgcgtaca
      961 cgcaaacccc cctctttgcc gaggccctca tccgcaataa attttttcac ttcagaaatt
     1021 gctttaggag tcagcgtcag ttgcattgct atctttgtct cactttctgg aattagttaa
     1081 ggtttatgtt gaaaatagtt ctacctttga ccggtgagct tgtcaactcg tgcttggaga
     1141 caaggtgggg ctgaagacat cgggttgaag acattgggct gaagacatcg ggctcagggc
     1201 ttggggctga agactcgcaa gctcggggct gaaga
//
LOCUS       DAOQDO010002275         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_142991_length_1235_cov_1.411017, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002275 DAOQDO010000000
VERSION     DAOQDO010002275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142991_length_1235_cov_1.411017"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1235
                     /locus_tag="PKZ53_26605"
     CDS             <1..>1235
                     /locus_tag="PKZ53_26605"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44083.1"
                     /translation="RFTRLQARSTLTPETEAGFRAEIETISKEYEQIEDTLRRTSSRY
                     TALSQPKPLTVSEIQSRIVAPDTVLLEYFLGEGRSFVWVVTPDSLTTVQLPKKAEIER
                     LARQAYDLMAETGDTNRTIRLDSIQSEQTQVALNEALSKLSRIILEPVASHLGNKRLL
                     IVPDGVLHFIPFAALPVGNAQSKPEPLLTSHELVVLPSASALDVLRTETLNRPKPTKE
                     LAVVADPVFEPTDGRLPKQVALSATALSTDQKLDRNRQLRLEKTKSSIQEVGIADEAN
                     QIPRLPGTRREAESILKLVPKTKAAHAFDFTASRSFFDRPDLDQFRLLHIATHGFINS
                     ERPEFSGLIFSMFDQQGKTQNGFLLLPDVFNLKLNTDLVTLSACQSGLGKEIKGEGMV
                     GLTQGFLYAGTSRVLVSLW"
BASE COUNT          307 a          346 c          293 g          289 t
ORIGIN      
        1 atcgcttcac ccgtctgcaa gcacgttcca cactgacccc agaaaccgaa gctgggtttc
       61 gggctgaaat cgagacaatt tccaaagaat atgagcaaat cgaagacacc ctccgccgaa
      121 ccagctcacg ctataccgct ttatcccagc ctaagccgct taccgtcagt gaaattcaat
      181 cccgaattgt cgcgcccgat acagtacttt tggaatattt tctaggggag ggccgcagtt
      241 ttgtgtgggt agtgactccg gattcactca ccacagttca acttcccaaa aaagctgaaa
      301 ttgaacgact tgcccgacag gcatatgact tgatggctga aacgggtgac accaatcgta
      361 cgattcggct ggattccatt caatcagaac agacgcaagt tgctctgaat gaagcccttt
      421 ccaaattgag ccgtataatt ttggagccag tggcctcaca cctggggaac aaacgattgc
      481 tgatcgttcc agatggcgtt ttgcatttca ttccatttgc agcgcttcca gttgggaatg
      541 ctcaatcaaa acctgaacca ctcctgacca gtcacgaact ggtggttttg ccttcagcct
      601 cggccctcga cgtcctgcga actgaaaccc tcaatcgtcc caaaccgacc aaagagctgg
      661 cggtggtggc agacccggtg tttgagccga cggatggccg gctccccaaa caggttgcgc
      721 tctcggcaac tgccctgtcc accgaccaga aacttgaccg gaatcgtcag cttcgcctgg
      781 aaaaaaccaa atcctcgatt caggaagtgg gtattgcgga tgaagccaac cagattccac
      841 gcctgcctgg gacacgccgc gaagctgaat caattctcaa gctcgttcca aaaaccaaag
      901 ccgcgcacgc ctttgatttc acagccagtc ggagcttttt tgatcgcccg gacctcgatc
      961 aatttcggtt gctccatatt gccacgcacg ggtttatcaa cagcgagcgg cctgaattct
     1021 caggattgat tttttcaatg tttgaccagc aaggaaaaac tcaaaatggg ttcttgctcc
     1081 tgccagacgt gtttaatctc aagctcaaca ccgatctggt caccctttct gcctgtcaaa
     1141 gcggtctggg caaagaaatt aagggtgaag gcatggtcgg gttaacccag gggtttctct
     1201 atgccggaac atcgcgggtc ctggtcagct tgtgg
//
LOCUS       DAOQDO010002276         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143055_length_1234_cov_2.977099, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002276 DAOQDO010000000
VERSION     DAOQDO010002276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143055_length_1234_cov_2.977099"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1234)
                     /locus_tag="PKZ53_26610"
     CDS             complement(<1..>1234)
                     /locus_tag="PKZ53_26610"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ44084.1"
                     /translation="VCDKGGQCELQDMAFDHGTVYADYMVQKNDKVEERLSPFIAYDE
                     QRCVKCYRCIRVCEEWMDVHALTKVFRGTHEMIGFYGNDLSCEQCGNCIEVCPVGALL
                     SVDSRFKSRPWDMKETVTTCTYCADGCQINLGVRGEEYVRVASKDMTGINGEFLCVKG
                     RYGSAFISSPERLKTPLIRKSGQLVPATWDEALKHAASRLNDVRRASGDASVAIIGSP
                     RITNEASYVLSRFGKALNTSNVGHFRSMELGEFFANASAPIATHAEMQAATTVLMIGA
                     DPTEESPLTGFSVRFAERKHDAQMLVVNSRPIKIARRQADNFLHIRPNAESAIVQALV
                     DETSVEAMAKAAGVPVAELKAVREKVLKATNLLIVFGPSVQGAALKALARLGSLVQDE
                     TEKQVHYVPMAQYNNSVGA"
BASE COUNT          262 a          352 c          340 g          280 t
ORIGIN      
        1 aagcaccaac cgagttgttg tactgagcca tcggcacata atggacctgc ttttcagtct
       61 catcctgaac caggctgccc agacgagcca gggctttgag cgcggcgccc tgaaccgatg
      121 gaccaaagac aatcagcaga ttggtggctt tcaatacttt ttcgcgaacg gctttgagtt
      181 cggcaaccgg cacgccagcc gctttggcca tggcttcgac gctggtttca tcaaccaggg
      241 cctggacaat cgcgctttcg gcatttggac gaatatggag gaagttgtca gcctgacggc
      301 gggcaatctt gatcggacgg gagttgacca ccagcatttg cgcgtcgtgc ttgcgttcgg
      361 caaagcgcac gctaaacccg gtgagcgggc tttcttcggt cgggtcggcc ccgatcatca
      421 gcaccgtggt ggccgcctgc atttcggcgt gggtggcaat cggcgcggag gcattggcga
      481 aaaattcgcc caattccatt gatcggaagt gcccaacatt tgaggtgttg agggcttttc
      541 caaaccgtga caggacatag ctggcttcgt tggtgatgcg aggggaaccg atgatggcca
      601 cgctggcatc accactggcc cggcgaacgt cattgagtcg gctggctgca tgtttgagcg
      661 cttcgtccca ggtcgccggt actaactggc cacttttgcg aatcaacggc gtcttcaatc
      721 gttccggact gctgataaaa gcgctgccgt agcggccctt gacgcacaaa aactcgccgt
      781 tgatgcccgt catgtccttc gaagcaaccc ggacatactc ttccccgcgc acgccgaggt
      841 tgatctggca accgtcggca cagtaggtgc aggtggtcac ggtttccttc atatcccatg
      901 ggcgggattt aaagcgtgaa tcaaccgaaa gcagggcgcc caccgggcag acttcaatac
      961 aatttccgca ttgttcgcag cttaaatcat tgccataaaa gccgatcatt tcgtgggtgc
     1021 cacgaaagac cttggtcagg gcgtggacat ccatccactc ttcgcacacc cgaatgcagc
     1081 gatagcattt cacacagcgc tgttcgtcat aggcgataaa tggtgacagg cgttcttcaa
     1141 ccttgtcatt tttctgcacc atgtaatcgg catagaccgt gccgtggtca aacgccatgt
     1201 cctgcaattc acactggccg cctttgtcac acac
//
LOCUS       DAOQDO010002277         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143097_length_1234_cov_2.449534, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002277 DAOQDO010000000
VERSION     DAOQDO010002277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143097_length_1234_cov_2.449534"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKZ53_26615"
     CDS             complement(<1..266)
                     /locus_tag="PKZ53_26615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44085.1"
                     /translation="MDILKNVRKQVDQLKLNREIEQLSRLVQENPQNAEALRRLAETY
                     QAANNVDAAISHFIQLADLYRQTKQHELALAFYRKVERMVDSDHR"
     gene            367..543
                     /locus_tag="PKZ53_26620"
     CDS             367..543
                     /locus_tag="PKZ53_26620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44086.1"
                     /translation="MNPAETATNDLRLQLAIPARLVASISLQLMTLARTIAGDRPRQS
                     LSCNLLLTTLVVHV"
     gene            complement(808..>1234)
                     /locus_tag="PKZ53_26625"
     CDS             complement(808..>1234)
                     /locus_tag="PKZ53_26625"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ44087.1"
                     /translation="DESLARSLVLDLVSKDPLVRSSAALALGKLQWLECATTLIEFLR
                     ADHHWVRYAAAVALGEMLEKRSVDPLCELMINDEDFLVRAGAARSLGKIGDRRAIKAL
                     RRGAVKDRNELVRADCLESLRIIWGGSDDESGPASGGVN"
BASE COUNT          321 a          331 c          315 g          267 t
ORIGIN      
        1 cgatggtcgc tatcaaccat gcgttccact tttcggtaaa aagccagcgc cagttcatgc
       61 tgtttggttt gacgatacag atcagccagt tggataaagt gggagatggc cgcgtcaacg
      121 ttgttggccg cttgataggt ctcagccaga cggcggaggg cttcggcgtt ctggggattt
      181 tcctgaacga gacggctgag ttgctcaatc tcccgattga gtttcaactg atctacctgt
      241 ttgcgaacat tcttaaggat atccatagca acgtgcggct accttccatt gaaagtctct
      301 aaggcttgcc aggaatacat caagcccaaa aggctgaacc ggttctcacc tgcggcccag
      361 ctcacaatga acccagcgga aacagcaacc aacgacctcc ggttgcaact ggcgattccg
      421 gcaaggctgg ttgcctccat cagtctgcag ttgatgactc tggcacggac cattgcaggt
      481 gaccgtccca ggcaaagtct gtcgtgcaac ctgttactca caacgttggt tgtgcacgtt
      541 tgaagaaaac tcagtgaaga aaacaaagat gttttgtttt cagggaaata tcggcaagtt
      601 tagggttcca ggttgtggag caaatacgag gggcgtgcgg taaagcaagc caggtggctg
      661 gaattataga atgaagaggg ccaaatgcaa ggttgacaac acttaaactg gtttccagcc
      721 gggttgtcaa gcctgatcag aaccaaacga caaagccatc caggctagaa accagaccaa
      781 aaaccagcac cggcaaaagg cggaaaccta attcactcct ccacttgccg gaccagattc
      841 atcgtcggat ccaccccaga taatccgaag tgattcaaga cagtcagcgc gtaccagttc
      901 attgcgatct ttgacggcgc cgcgtcggag cgctttaatt gcccgacggt caccaatttt
      961 ccccagcgaa cgggcagccc cagcccgaac taaaaaatct tcgtcattga tcatcagctc
     1021 acacagtggg tcaactgacc gtttttccaa catttcgccc aatgcaacgg cggcggcata
     1081 gcggacccaa tggtgatcgg cccgtaaaaa ttcgatcagg gtggtggcac attcgagcca
     1141 ctgcagtttt cccaaagcca gggcggcaga acttcgcacc agcgggtctt ttgaaaccag
     1201 atccagcacc agcgacctgg ccagggattc atcc
//
LOCUS       DAOQDO010002278         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143283_length_1233_cov_3.899830, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002278 DAOQDO010000000
VERSION     DAOQDO010002278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143283_length_1233_cov_3.899830"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKZ53_26630"
     CDS             complement(<1..630)
                     /locus_tag="PKZ53_26630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44088.1"
                     /translation="MITDLFNPWHGWRHRDWEDEFEVSREQLPVITQEHIVNIYEPGK
                     SWLGFWFPLRNKSITDTSELSEGWIHESFPGDNPRFFQELVCELQELAPSLVFLRNLK
                     QLAMLNRTSECSDSLLLKFSLQSERIPAPDVQPGTVMSVRGDLVLNSGNGDTSFHYCG
                     FAGRLQEDATTSQLKRSLGWPKVVKRTQGYNQASLPVKGDPHFATLISSH"
BASE COUNT          340 a          294 c          275 g          324 t
ORIGIN      
        1 gtgacttgag atcaacgtgg caaagtgagg gtcaccttta accggaaggc tcgcctggtt
       61 ataaccttgg gtgcgcttca caactttagg ccagcccaga gaacgcttta gttgagaagt
      121 tgtagcatct tcctgtaacc gtccagcaaa accacagtaa tgaaaactcg tgtcaccgtt
      181 accagaattc aaaacgaggt caccgcgcac cgacatcacc gttccaggtt gaacatcagg
      241 ggctggaata cgctcacttt gcagcgagaa tttgagcaaa agcgagtctg agcattcact
      301 ggtccgattc agcatggcca actgcttcaa attcctcaga aaaacaagtg aaggagcaag
      361 ttcctggagt tcgcacacca actcctgaaa aaaccttgga ttatctcctg gaaaactttc
      421 atggatccac ccttcagata attcggaagt atctgtgatt gacttattcc tcaaggggaa
      481 ccaaaaaccg agccaggatt tcccaggctc ataaatattg acaatgtgtt cttgtgtaat
      541 gactggcagt tgctcccgac tcacctcaaa ttcatcctcc caatctcggt gcctccagcc
      601 atgccagggg ttgaaaaggt cagtaatcat tgggtaaggc cacccttgct tcaggttagt
      661 tttggctatg atgaaaaacg cttcgcacca ggcgaaaaca cttttcagcc cttttccaaa
      721 ccgaccaatt gaccgttctt cagttccttt tgttcccagg ttaatttgtg taatcgcatc
      781 tcgatcttct gacttgaaga caccatcatt gacaaccagg agccccggtc ctcgaaggag
      841 aacgtgtggt gcagccgggt caccaggcag ataaatgaga tcaagccggg ttgaaccagc
      901 atcctctgca ttttggatta attctttgag aatactccga tatgaaccat acccttgcag
      961 gagatctcga atactttcta tatcggcttc tgggggatgc cgaaccccac ctgagcgaaa
     1021 agcgctgaag ttgttatcct gcatgtcttt caacgtaagc tccttttggg aagtcagggt
     1081 aataacccaa taaccaaggg tgacaattgc caattttctc catcagttga atatttgcaa
     1141 tgtggaatta tgcagtgagt tgcgggaaaa taaaagagag aactggttgc gagttttgaa
     1201 aattgatcta aacctttgtt ttgtatatgt tag
//
LOCUS       DAOQDO010002279         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143308_length_1233_cov_2.943124, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002279 DAOQDO010000000
VERSION     DAOQDO010002279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143308_length_1233_cov_2.943124"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>1233
                     /locus_tag="PKZ53_26635"
     CDS             151..>1233
                     /locus_tag="PKZ53_26635"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ44089.1"
                     /translation="MFEKRARKDQKILVIEDSQTQAQKLVGFLEDQGFQCVHARTGVD
                     GLQAMQDSDPTLVVSDVLMPEMNGYDFCRAAKNDEALREIPIILLTSLSDTSDILRGL
                     ECGADNFITKPYSEDYLLTRIEYILTNRELRKRNRMQMGVEIEFAGKVHFITSERQQI
                     LDLLISTYEQAVHINDKLKRREEELEASILHLATLNAVGNTVNQSLDLEEILRDALEK
                     LIEILLIDGAGIFLREGDTLVLKANLGLPDELSRPDLVPNLAFPPQAGRPSVGQPLNP
                     AADDFPWFSVRSLNTPSSQFFMTVPLKAKNEIIGVIVLYMLQYQQFKQAENEWIQSIS
                     NQIAVATENARLYGLMKSRRIQEQATL"
BASE COUNT          341 a          275 c          317 g          300 t
ORIGIN      
        1 tgaaaaaagt tcagttgttt ttggcatgcc aggcaaagcg gttagtattg gagcggccca
       61 atttgtactc aatcccaatc agattgctca gtatctcaag gaaataaatg ccggtagcct
      121 caaccgatga gtttgcagag ggagcgcact atgtttgaaa aaagagccag gaaagatcaa
      181 aaaatactgg tgattgaaga tagccagacc caggcacaga agttggtcgg attcctggaa
      241 gaccaggggt ttcagtgtgt tcatgcccga accggggtgg atggtttaca ggcaatgcag
      301 gacagtgacc cgacactggt ggtcagtgat gtgctgatgc cggaaatgaa tggctatgat
      361 ttttgccggg ccgccaaaaa tgatgaggcc ctcagagaaa ttccaatcat tttactcact
      421 tcgttatctg atacgtctga tattttgcgc ggcctggaat gcggcgccga caattttatt
      481 accaagccct atagcgagga ttatttatta acccgcatcg aatacatcct caccaatcgg
      541 gaactgcgca aacgcaaccg gatgcagatg ggggtggaaa tcgaatttgc cggaaaagtt
      601 cactttatta cgtctgagcg acagcagatc ctggatttac tgatttcgac ctatgaacag
      661 gccgtccaca tcaatgacaa attgaaacgg cgagaagaag aactcgaagc ttcgatcctg
      721 cacctggcca cccttaacgc ggtagggaac accgtcaatc aatccttaga tctcgaagaa
      781 atcctgcggg atgcgcttga aaagttaatt gagattttgc tgattgacgg ggctggaatc
      841 tttctgcggg aaggcgacac gctggtttta aaagccaatc tgggattgcc agatgaactt
      901 tcacggccag acctggtgcc gaatctggct tttccgccgc aggccggaag accttcagtc
      961 ggtcaaccac tcaatccagc ggcagatgat tttccctggt tcagtgtcag gtctctcaat
     1021 acgccgagtt ctcaattttt tatgacagtt ccgctgaagg caaaaaatga aattattggg
     1081 gtcatcgtgc tgtatatgtt gcagtatcaa cagtttaaac aggctgagaa tgagtggatc
     1141 caaagtatca gcaaccagat tgccgtggcg actgaaaatg cccggctcta tggattaatg
     1201 aaatccaggc ggattcagga gcaggccacc ttg
//
LOCUS       DAOQDO010002280         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143312_length_1233_cov_2.869270, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002280 DAOQDO010000000
VERSION     DAOQDO010002280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143312_length_1233_cov_2.869270"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1215
                     /gene="gap"
                     /locus_tag="PKZ53_26640"
     CDS             205..1215
                     /gene="gap"
                     /locus_tag="PKZ53_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNJ44090.1"
                     /translation="MAIKVGINGFGRIGRNVLRTVLGDSEIEIVAVNDLTDAKTLAHL
                     LKYDSILGNLEATVTAPAEDCIEVNGHPIKVFKVRSPEELDWESVGAEVVVESTGLFT
                     KGEKAAGHLRGSVKKVIISAPAKGEDLTLVLGVNDHMYDPEKHRVLSNASCTTNCLAP
                     VAKVIHENFGIVKALMTTIHAYTNDQRILDLPHSDLRRARAAALSMIPTKTGAAQAVE
                     LVMPELKGKFDGLAVRVPTPNVSVVDVTFEVEKSTDKESVMSALKAAAEGPLKGILAY
                     TEEPLVSIDFRMNPNSSIVDGPLTRVIGGNMIKVMSWYDNEWGYSCRVRDLIKFMAAK
                     GL"
BASE COUNT          320 a          320 c          324 g          269 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac cccaagtgcc
       61 aaatggttct tgacttcatt ggggtcgctg ggataatcct agcctttctc gacaacccag
      121 aaaagcgaat gtttcacgcc ccatccatcg ctttgtcgca atccacacac tctatcattc
      181 aactgaattg ttcaggaggg tttcatggcc atcaaagtag gcattaacgg attcggtcgt
      241 atcggacgca acgtgctgcg tacggttctc ggtgactcag agattgaaat tgttgctgta
      301 aatgatttga ccgacgcaaa aacgctggcg cacctgctca aatacgactc gattttagga
      361 aatttggaag ccacggtcac ggctccggct gaagactgca tcgaagtcaa cggacatcca
      421 atcaaagttt tcaaagtgcg tagcccagaa gaactcgatt gggaatcggt cggtgcggaa
      481 gttgtggtcg aatccaccgg tttgttcact aaaggcgaaa aagcggctgg ccacctgcgt
      541 ggatcagtca aaaaggtcat catctcggcg cccgccaaag gcgaagacct gacccttgtg
      601 ctcggcgtga acgatcatat gtatgatccg gaaaaacacc gggttctgtc caacgcatcc
      661 tgtaccacca actgtctggc accggttgcc aaagtcattc atgaaaattt cggaatcgtc
      721 aaagccctga tgacgacaat ccatgcatac actaacgatc agcgcattct tgacctgcca
      781 cacagcgatc tgcgccgtgc ccgtgctgcg gcgttgtcaa tgattccgac caaaaccggt
      841 gccgctcagg ccgtggaact ggtaatgccg gaactcaaag gcaaattcga cggtctggcc
      901 gtgcgtgtcc caacgccgaa cgtgtcagtg gttgacgtga catttgaagt tgagaaatca
      961 actgacaagg aaagtgtcat gagtgctttg aaagcggcag cggaaggccc gttgaaaggc
     1021 attttggctt acacggaaga accgctggtt tcgattgact tccggatgaa cccgaactca
     1081 tccatcgtag acggcccgct cactcgggtc atcggcggca acatgatcaa agtgatgtcg
     1141 tggtatgaca atgagtgggg atatagctgc cgtgtccgtg acctgatcaa gttcatggct
     1201 gccaaaggac tgtaaattaa taaggtgata ggg
//
LOCUS       DAOQDO010002281         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143522_length_1232_cov_4.376381, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002281 DAOQDO010000000
VERSION     DAOQDO010002281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143522_length_1232_cov_4.376381"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..>1232
                     /locus_tag="PKZ53_26645"
     CDS             229..>1232
                     /locus_tag="PKZ53_26645"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ44091.1"
                     /translation="MSQPLFVRDLTDTERATLTEWLACDDTEKVHRAKVILYSDARKT
                     AYEIGELLSSHPDNLKKWIREFNRQGLEGIIVRKRGPQGKFSAEQINQILELYRQPPR
                     RLGYNFDYWTPQKLANIVMERGIVPAISHVTMRQILREAEGEESDASGAEILPFVSKS
                     EPASNKSAVTSSASTASELARESFAFVTSPEPLTDQPLTPGFPPPQIVDPPVQPEVSF
                     EAAFQQSQALMRRGNYVVAAGVFRSLLEQRKDLSAETEAKIRCWLSEALEGLGRYEES
                     LGVIECYEDPLWRSQLSESAYAWSRLRLGLAYARTGGNKAITRLKEALRIFEELEDFE
                     G"
BASE COUNT          319 a          305 c          304 g          304 t
ORIGIN      
        1 gtggcatgtg cgttgcagat actaccctgg taaaacaatt tttcccctga atgaatacat
       61 agtagtcaga tgtaagtatt ttcatctgag tgttctctgt actttccaga gcccgactgg
      121 acttgggtcg aagtgaatta cctgatgatt gacagatatc attgcttctg gtatttaaat
      181 cagaactacc atagttagat acaaactttc catcagagtt taaaattaat gtcacagcca
      241 ctatttgtcc gcgatttaac tgataccgaa cgggcgaccc tcaccgagtg gctggcctgt
      301 gacgatacgg agaaagtaca tcgggcgaag gtcattcttt actctgacgc ccggaaaaca
      361 gcttatgaaa ttggggaact gctgtcttcc catcctgata atctcaaaaa gtggattcga
      421 gaattcaatc gccagggcct tgaaggaatt attgtccgaa aacgaggtcc tcagggaaaa
      481 tttagcgccg agcaaattaa tcagatttta gaactgtatc ggcagccacc ccgccgcttg
      541 ggctataact ttgactattg gactccccag aagctggcca atattgtcat ggaacgtggg
      601 attgtcccag ccatcagtca tgtcacaatg cgccaaatct tgcgcgaggc tgaaggtgag
      661 gaatcagacg catctggggc agaaatttta ccgtttgttt ccaaaagcga gcccgccagt
      721 aataaatcag ccgtcaccag cagtgcatct actgcctctg aactcgcccg agagagtttt
      781 gcctttgtta cgtcccctga accgttaact gaccagccat tgactccggg gtttccacca
      841 ccccagatcg ttgaccctcc agttcagccc gaagtcagtt ttgaagccgc ctttcaacaa
      901 agtcaggcgc tcatgcggcg tggaaattat gtagttgcag ccggggtttt ccggtcacta
      961 ctggaacaac gcaaagacct ttcggctgaa accgaagcta aaattcggtg ctggctgagc
     1021 gaagcattgg agggcctggg acgctatgaa gaatcgctag gggtgattga atgttatgaa
     1081 gacccactct ggcgcagcca gctcagcgag agcgcctatg cctggagtcg tctccgcctg
     1141 ggcctggcct atgcccgcac tggcgggaac aaagccatta cccggctgaa ggaagcactc
     1201 cgcatctttg aagaacttga agattttgaa gg
//
LOCUS       DAOQDO010002282         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143541_length_1232_cov_3.441801, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002282 DAOQDO010000000
VERSION     DAOQDO010002282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143541_length_1232_cov_3.441801"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PKZ53_26650"
     CDS             complement(<1..1224)
                     /locus_tag="PKZ53_26650"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNJ44092.1"
                     /translation="MPTGIALSSSGTLSGTPTASGTSNFTIRVIDNNGCVVTPSYSLK
                     VDGPECPTITVNPASLPGGTIGTAYNQVISATGGTAPYSFTVLSGSLPPGVTLATNGT
                     LSGTPSSAGSFTFIVRATDTPGCTGQRTYTVAITGSGGCGTISLNPSVLPNGVVGTAY
                     NQTVIASGGNGSYAFGVISGEIPPGIVLSPTGTFSGTPTTAGSYSFVLAATDSNSCSG
                     SLSYTIVIDEPGGGCNSSISLSPKVLPLGQEFQVYNQPLTATGGTAPYFFQVTDGVLP
                     EGITLTPEGVLFGTATLAGDFTFTVLVTDASNCTLNQTLTVTIIPGGCQPFSLNPATL
                     QNGTAGEPYQQVFGVGGGTAPYQFSIIQGGLPSGLTLSQSGTISGVPAVDGVSTFTVQ
                     VIDSLGCRTRQTYSIT"
BASE COUNT          322 a          329 c          315 g          266 t
ORIGIN      
        1 cgtaatcgaa tatgtttgcc gagttcggca ccccaaacta tcaatcacct gaaccgtaaa
       61 ggtcgaaaca ccatccacgg cgggcacacc tgaaatcgtt ccactctgtg aaagtgtcaa
      121 tccagatggc aacccgccct gaataattga aaactgatag ggggcggttc ccccacccac
      181 accaaagact tgttgatagg gttcgccggc agttccattc tgcaacgttg ccggattgag
      241 cgaaaacggc tggcaaccgc cgggaatgat ggtcacggtt aatgtttgat tcagagtaca
      301 gttactggca tcggtcacca aaacagtgaa tgtaaaatcc ccagccaggg tcgccgtacc
      361 aaataaaaca ccttcggggg ttaaggtaat accttcgggc agcacgccat ctgttacctg
      421 gaaaaagtag ggggccgttc ccccagtcgc ggtcagcggc tggttataga cctgaaattc
      481 ctgacctaac ggcaaaactt tgggacttaa actaattgag gagttacacc ctccgccagg
      541 ttcatcaatc acaattgtgt agctgagact tccggaacaa ctgtttgaat cagtcgcggc
      601 caggacaaat gaataactgc ctgctgtggt tggagttccc gaaaatgttc cagtgggtga
      661 gagcacaatc cctgggggaa tctctccgct aatgacacca aacgcatagg aaccatttcc
      721 acctgaagca atcacggtct ggttatatgc cgttccaacc accccgttcg gtaagactga
      781 tggattgagg ctgatcgtac cgcatccacc cgaaccagta atcgccaccg tgtacgtccg
      841 ttgaccagta cacccagggg tatcggttgc gcgcacgata aaggtaaaac tcccggcact
      901 tgatggcgtg ccagacaggg tgccgttggt cgctagcgtc actcctgggg gaagactgcc
      961 tgacaaaaca gtgaacgaat aaggtgcggt cccacctgtg gcactgatca cctgattgta
     1021 agccgttccg atggtgccac ccggtaatga agctggattg acagtaatgg tcgggcattc
     1081 aggtccatca accttgaggg aataacttgg cgttacaacg cacccgttgt tgtcaatcac
     1141 ccgaattgtg aaatttgaag ttcccgaagc cgtcggagtt ccagaaagtg taccgctgct
     1201 cgataaagca ataccggtcg gcaaggcccc cg
//
LOCUS       DAOQDO010002283         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143546_length_1232_cov_3.372133, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002283 DAOQDO010000000
VERSION     DAOQDO010002283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143546_length_1232_cov_3.372133"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1232
                     /locus_tag="PKZ53_26655"
     CDS             <1..>1232
                     /locus_tag="PKZ53_26655"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNJ44093.1"
                     /translation="RGLDGDVLVFLPGAAEIRKCRDACEPLAKLHNWLVLPLHGDLTP
                     EEQDQAVRATEQTKIILATNVAESSVTIDGVVTVIDSGLARVAKTSPWTGFSSLEVAK
                     VSRASVIQRAGRAGRTRAGRCLRLFTRFDFEARPAQDLPEIKRTDLAETVLALRTLGI
                     DPTLLDWFDPPSEAALSAADTLLQQLGATDNENSLTKTGRDMALLPLHPRFSRLLVET
                     KRQGIAAEGCILAALLGERDIRVDSRTQLHSSPQSRRGATSSSSDVLEQFDLFCEAER
                     MRFVPDRVRMLGLDVGTVRRVERARQQVHRLITPDRNTAPAVSPGNLDEVLLKAVLVA
                     FPDRVGRRRARTSKEFSQKVVLSSGGEGILAETSSVRQAEFLVAVQAEDQAGKGVLIR
                     VASAIEPEWLLEFFPDRI"
BASE COUNT          260 a          311 c          381 g          280 t
ORIGIN      
        1 atcgagggtt ggatggcgat gtgctggtgt ttcttccagg tgccgccgaa atcagaaaat
       61 gccgcgacgc ttgcgaacca ctggcgaaac tccacaactg gctggtgctg ccgctccacg
      121 gagacttaac ccctgaggag caggatcagg ctgtccgagc gactgaacag accaaaatca
      181 ttctggcaac gaatgtggcg gaatcatcgg tcacgattga tggcgtggtg acggtgattg
      241 attcggggct ggctcgggta gccaaaacgt cgccctggac cgggttttca tcgcttgaag
      301 tcgccaaagt cagtcgtgct tcagtgattc aacgggctgg acgggctggg agaacccgag
      361 ctgggcgatg tctccgattg tttacccggt ttgattttga ggctcgccct gcgcaggacc
      421 ttccggaaat caaacggact gatctggctg aaactgtgct ggcattgcga acactgggaa
      481 ttgacccgac gctgctggac tggtttgacc caccgtccga agcggcattg tcggcagccg
      541 atacattgct gcagcagctt ggtgcgactg ataatgaaaa ttccttaact aaaactggcc
      601 gggatatggc gttgctgcca ctgcatccac gtttttcacg cttgctggtc gagactaaac
      661 gacagggaat tgctgcagaa ggctgcattc tggcggcact gctgggggaa cgcgacattc
      721 gggtggactc acggacgcag ctccattcgt cacctcaatc acggcgtggt gcaacgagtt
      781 cttcctccga tgtccttgag caatttgatc tcttttgcga agcggagcgg atgcggtttg
      841 ttcccgaccg ggtgcgaatg ctgggtcttg atgttggaac cgttcggcga gttgaacgcg
      901 cccggcagca ggtgcaccgt ctgataacac cggatagaaa cacggctcct gctgtttcac
      961 ccggcaatct ggatgaggtc ctgttgaaag cggttctggt ggcctttcca gatcgagttg
     1021 ggcgacgacg ggcacgaacc tcaaaagaat tcagccaaaa ggttgtgctt tcaagcggcg
     1081 gtgaaggaat cctggctgaa accagttcag tgcgtcaggc tgagtttctg gtggcagttc
     1141 aggccgaaga tcaggcggga aaaggtgtgc tcattcgggt ggcttcggcg atagaaccag
     1201 aatggctgct cgaatttttc ccggaccgga tc
//
LOCUS       DAOQDO010002284         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143627_length_1232_cov_2.233645, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002284 DAOQDO010000000
VERSION     DAOQDO010002284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143627_length_1232_cov_2.233645"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKZ53_26660"
     CDS             complement(<1..401)
                     /locus_tag="PKZ53_26660"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ44094.1"
                     /translation="MRPIVWLWLALIGLSPVSVWSVPRQTSSRGITIVNEKKQEYMNV
                     TLYKLKGDQEISVDPNKTTFGGGEQFKVSLEPFFDGYLYVLNEDPAGDLTVLYPRPQD
                     QDTVNAGKTITLPTFQVDNEPGDEALQIFVSP"
     gene            complement(519..1091)
                     /locus_tag="PKZ53_26665"
     CDS             complement(519..1091)
                     /locus_tag="PKZ53_26665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44095.1"
                     /translation="MTIKENLRKGGHLIATLASLVSAQQVDVELLADQWEAVTSEMMS
                     GGQVTPDSFQMTLASAGVNEMPYHSAQQLAQEFKGSQLHKMTQVFTSDDKITPYLNSL
                     NVLCHSYTAKYSTSITQVASGPSENHKLTLKRLKALKELTEALGKLGNALKNRREEKE
                     KLDDLLKTAEEERKFGQKRPYEIPRGIDER"
BASE COUNT          307 a          260 c          297 g          368 t
ORIGIN      
        1 ggcgacacaa aaatctgaag ggcttcatcg cccggttcat tgtcaacctg gaaagtgggc
       61 agggtgatgg tttttccggc attgacggta tcctggtctt gcgggcgggg atagaggacg
      121 gtgagatcgc cggcagggtc ttcgttcagg acatagagat agccgtcaaa aaatggttcc
      181 aggctgactt taaattgctc accgccgcca aaagtcgttt tgttgggatc aaccgagatt
      241 tcctggtcac cttttaattt atacaaggtg acattcatat attcctgttt tttctcattg
      301 acgatagtga taccacggga tgaagtttgc cgcggtaccg accaaaccga gaccgggctg
      361 agtccaatca gcgccagcca aagccaaacg atgggtcgca tagggtgaat tcctcctggg
      421 ggtggatgta aatcggaaat caactatttg atttttggga atgtaacttt catgttgctg
      481 caaagagcta gaataaaaga aaatatagtg aaattgcgtt atcgctcatc aattccacgg
      541 ggtatttcat agggtcgctt ttgaccaaac ttccgttctt cctctgcggt tttgagcaag
      601 tcgtcaagtt tttccttttc ttcccttcga tttttcaagg cattacccaa tttacctaat
      661 gcttcagtaa gttctttgag cgcctttaat cttttaagtg ttaatttgtg attttcagat
      721 ggtcccgaag ctacctgggt gatcgaagtt gaatatttgg cggtatatga atgacacaaa
      781 acattcagtg aattgaggta tggcgtaatt ttgtcgtctg aagtgaaaac ctgggtcatt
      841 ttatgcagtt gagatccttt gaattcctgc gccagttgtt gtgccgaatg gtatggcatt
      901 tcattgaccc cagcgcttgc gagtgtcatc tggaatgaat ccggggtcac ctgcccacca
      961 gacatcattt cactcgtgac tgcctcccat tgatctgcaa gcaattcaac atcaacttgc
     1021 tgggcagaca ctaaggaggc taaggttgca atcagatggc cgcccttcct caaattttct
     1081 ttgattgtca tattgaactc caagtttgtg tggatctaaa cgggttatga atcaagcagt
     1141 gtcttgatcc gttcaagaat ttcgtcaaat tggctgggga aattccgttg gagtctggtg
     1201 gcatccagaa aaggttgcaa ataatcctca ac
//
LOCUS       DAOQDO010002285         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_143881_length_1231_cov_2.062925, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002285 DAOQDO010000000
VERSION     DAOQDO010002285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143881_length_1231_cov_2.062925"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1134)
                     /locus_tag="PKZ53_26670"
     CDS             complement(97..1134)
                     /locus_tag="PKZ53_26670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ44096.1"
                     /translation="MKPEKLKAVTWGVIVTLLLTGMIVAGSRNLTHFDAALVGYTFAV
                     LFAAFGLTYRYSMWLQRPPTAMYWKRGWQVFFRPQYLIQNAIHWVKKVSSDFAFNQFI
                     FRRGKARWGAHWFIMWGCVMAIAITFPLVFGWIHFETIPENLEWYRTFVFGFPTFSFP
                     VHSLFGFLIFHGLVWASFAVILGVMLAMRRRMRDHGAAAIQQFGEDFLPLILLFSVSV
                     TGLMLTASYSWMKGYGYEFLAILHAITVIFTLLWLPFGKFFHIFQRPAQLGVSFYKEI
                     GTREEQAQCRRCGEAFASKMHVQDLIQVQQQLGFTYEIDSEVEHYQWICPACRRRMLA
                     IAQGQVWRNQG"
BASE COUNT          346 a          322 c          308 g          255 t
ORIGIN      
        1 tcgattgctc cgagcttgcg agtcttcagc ccctagcccc tagcccctag cccctagccc
       61 gatgccttca gcccgatgtc ttcagccctg gttttttcat ccctggtttc tccagacctg
      121 cccctgcgca atggccagca tccgtcttcg gcaggccggg caaatccatt gataatgctc
      181 aacttctgag tcaatttcgt acgtgaaccc cagttgctgt tgaacctgga ttaaatcctg
      241 gacgtgcatt ttggaggcga aggcttcacc acaccgacga cactgtgcct gttcttcacg
      301 tgtgccgatt tctttataaa agctgacccc aagctgggcc gggcgttgaa aaatatggaa
      361 aaatttaccg aagggaagcc agagcaaggt aaaaataacg gtgatagcgt ggaggattgc
      421 cagaaattca tagccatagc ctttcatcca cgaataactg gcggtcaaca tcaatcctgt
      481 gacgctgact gaaaacaaga gaataagcgg cagaaaatct tcgccaaatt gctggattgc
      541 ggcggcaccg tggtcacgca tccggcggcg catcgcgagc atgacgccta aaatcacggc
      601 aaacgaggcc cacaccagcc cgtgaaagat caggaagcca aacagggaat ggactggaaa
      661 agaaaaggtt ggaaagccga aaacaaaagt tcgataccat tccaggtttt cagggatggt
      721 ttcaaagtga atccacccaa aaacaagggg gaaggtgatg gcaattgcca tcacacatcc
      781 ccacataatg aaccagtgcg caccccagcg cgctttgcct cgacgaaaaa taaattgatt
      841 gaatgcaaaa tcagaactga ccttcttcac ccagtggatg gcattctgga tcagatactg
      901 agggcgaaaa aacacctgcc agccacgttt ccaatacatg gcggtcggtg ggcgttgcag
      961 ccacattgaa taccggtagg tcaatccaaa cgcggcaaac agaacagcaa acgtgtagcc
     1021 aaccagtgcg gcgtcaaagt gggtgaggtt tcgcgaacca gccacaatca tccccgtcaa
     1081 cagcagtgtg acgataactc cccaggtgac agctttcagt ttctcaggtt tcatacagat
     1141 ccagggttcg gggttcaggg ttcagggttc agggttcagg gttccgagca gaggaaaaaa
     1201 ggacagcaag gacataaaaa tgatttcttc g
//
LOCUS       DAOQDO010002286         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144035_length_1230_cov_3.269787, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002286 DAOQDO010000000
VERSION     DAOQDO010002286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144035_length_1230_cov_3.269787"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKZ53_26675"
     CDS             <1..611
                     /locus_tag="PKZ53_26675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44097.1"
                     /translation="YTFRQEVRIQTLNFDDRPDGEYYRVSDILFTDDGRRTERIVRFP
                     QSTLQRLLITPNDLRDLASVQPFSLTTEDLPKYTVTYVSKERLDEIDTYVFDVRPKSI
                     PKYERDGGERVFQGRIWVDDQDLMIVKTFGKALPEGKERFPRFETYRENIDGKYWFPT
                     YTYALDTLDFEGGSSIRMKMEVRYTNYKQFKTDIKILDDDEG"
     gene            complement(694..1071)
                     /locus_tag="PKZ53_26680"
     CDS             complement(694..1071)
                     /locus_tag="PKZ53_26680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44098.1"
                     /translation="MTWHTKYCPELGIVETCYEGRISKDELQAAARHTLQLGQQHETN
                     WFLGDCTNLLGGHSIIDLYGLVELLAAHELSPRFREAILLPGLQASVRDVEFWETACW
                     NRGITVRLFDQREAALKWLINEK"
BASE COUNT          324 a          288 c          299 g          319 t
ORIGIN      
        1 attatacctt ccgccaggaa gtccgaattc aaaccctgaa ttttgatgat cgtccagatg
       61 gtgagtatta ccgggtttcc gatattctat tcaccgacga tggtcggcgg actgagcgca
      121 ttgtccggtt tccacaatca accttgcagc gcttattgat cacccccaac gatttgcgtg
      181 atctggccag cgtccagccg ttttcgctca caaccgaaga cctccccaag tacactgtga
      241 cctatgtcag caaagagcgc ctggatgaaa ttgataccta tgtctttgat gtgcgaccaa
      301 aaagcatccc caaatatgaa cgtgatgggg gcgaacgagt atttcaaggc cgtatctggg
      361 tagatgacca ggatttaatg attgtcaaaa cctttggtaa agctttgccg gaaggaaagg
      421 aacgcttccc gcgatttgaa acctaccgtg aaaatattga tggaaaatac tggtttccga
      481 cctataccta cgcgctcgac acgcttgact tcgaaggtgg ctcttcaatt cggatgaaaa
      541 tggaagttcg gtataccaat tacaaacagt tcaagaccga catcaagatc ctggatgatg
      601 acgaagggtg acctggaatg aaaagttgag aatgaagaat ggagaatgga gaaagaagaa
      661 aaagagggtt aagtcctgtt ctttcgctat tcttcatttt tcatttatca gccatttgag
      721 agctgcctct cgttgatcaa aaagacgaac tgtaattccc cgattccagc acgcagtttc
      781 ccaaaactca acatcccgta ctgatgcttg cagcccaggc agcaaaatag cctcccggaa
      841 ccggggtgac agctcatgtg ccgccagcaa ttccaccaat ccatacaaat caatgatcga
      901 gtgtccaccc aggaggttgg tacaatctcc caggaaccag ttcgtttcgt gctgttgtcc
      961 aagttgaagc gtgtggcgtg ctgcagcttg aagttcgtct ttagagatcc gtccttcata
     1021 gcaggtttcg acaatcccta gctcagggca gtactttgtg tgccaggtca tagttgattt
     1081 gccccttttg attgtaagcg gatgtgggta ccagcagatg actgagccac tggttttagt
     1141 gccagtgcgc tgggagggat gactgggtga tcgttcactc tatgactgtt gtggctttga
     1201 tttcaatcaa aaattcaggc ctgacgaggg
//
LOCUS       DAOQDO010002287         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144065_length_1230_cov_2.670638, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002287 DAOQDO010000000
VERSION     DAOQDO010002287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144065_length_1230_cov_2.670638"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..454)
                     /locus_tag="PKZ53_26685"
     CDS             complement(140..454)
                     /locus_tag="PKZ53_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44099.1"
                     /translation="MITYRAMYKFVENGVHGEVLDFPGVITFGSSLEETRRLLAGALV
                     DMAETCIFEGESLPSPDPTCSDSESDLEEPIHLLLTAANQVEIIPKEVTPFRAQGIGL
                     GV"
     gene            complement(474..>1230)
                     /locus_tag="PKZ53_26690"
     CDS             complement(474..>1230)
                     /locus_tag="PKZ53_26690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ44100.1"
                     /translation="LTYVYGPVPSRRLGTSLGINPLPVETKVCNFDCPYCECGWTDSD
                     RPAGKMPTVEQLVEELELKLKECQKHGIELAAITFAGNGEPTLHPKFELLIDKTCELR
                     DRYAPNAKVTVLTNATRLKFAPARRGLLKADVRQMKLDAGTEATFRQVDRPYGKLTLG
                     KIVEDICDFEAPVMIQSMFFRGEVDGEVIDNSRPEEVSQWLAHLKRIKPTAVHLYSLD
                     RPTAAKNLHRVSREELENIAAQVRTLGIPAETF"
BASE COUNT          285 a          321 c          309 g          315 t
ORIGIN      
        1 ggttggggct caccaggcca cggccttgag ccgccaacag cagggtttct cgaataaata
       61 ccagatcgtc catatttttt tagggttcag ggttcagggt tcagggttca gggtaaatta
      121 ttggaaaaat tcggttttcc taaaccccaa gcccgatgcc ctgagcccga aatggtgtga
      181 cttctttagg aatgatctca acctggtttg cagccgtgag taggagatgg atcggttctt
      241 ccaaatcaga ttctgagtca gaacaagttg ggtcaggaga tggcaggctc tcgccctcga
      301 aaatgcaggt ttctgccata tcaaccagag cgcctgccag caggcggcga gtttcctcca
      361 gcgaagaacc aaatgtaatt actcctggaa aatcaagtac ttctccatga accccattct
      421 caacgaattt atacatcgca cgataagtaa tcattcgtcc ctcttctaaa tcctcaaaac
      481 gtttcggctg gaatgcccaa tgttcgcacc tgggctgcaa tattttccag ttcttcacgc
      541 gagacgcggt gtaaattttt agcggctgtc gggcggtcca ggctgtagag gtgcaccgcc
      601 gtcggtttga tccgtttcag atgtgcaagc cactggctca cttcctcggg tctggagttg
      661 tcaatcactt cgccgtcaac ttcaccccga aaaaacatgc tctgaatcat caccggcgct
      721 tcaaagtcgc agatatcctc gacgattttt ccaagcgtca gtttgccata gggccgatct
      781 acctggcgaa aggtagcttc ggtgccagca tcgagtttca tctggcgcac atcagctttg
      841 agcaatccgc gccgggccgg ggcaaatttg agtcgggtgg cattggttaa gacagtcact
      901 ttggcgtttg gggcataacg gtcgcgaagt tcgcaggttt tgtcaatcag aagttcaaat
      961 ttcggatgca acgtcggctc gccattcccc gcaaacgtga tggccgccag ttcgattccg
     1021 tgtttctggc attccttgag tttgagttca agttcttcga ccagttgctc gacagtgggc
     1081 attttcccag caggccgatc agaatcggtc cagccgcatt cgcaataggg acaatcaaaa
     1141 ttgcagactt tggtttccac cggcaatgga ttgattccaa gactcgtccc cagtcgccgc
     1201 gatggcactg gcccatagac ataagtcaaa
//
LOCUS       DAOQDO010002288         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144069_length_1230_cov_2.547234, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002288 DAOQDO010000000
VERSION     DAOQDO010002288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144069_length_1230_cov_2.547234"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..>1230)
                     /locus_tag="PKZ53_26695"
     CDS             complement(68..>1230)
                     /locus_tag="PKZ53_26695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44101.1"
                     /translation="LVRLSSKIAGKFQTISFNASTDLNPAVFQPFDIIYLMMIQVVEA
                     TKEKTGKSPSSQLLGEIKDWFTTKTQKENRTTMFGAEISAGAGADDKSLLANFLGLIA
                     AIKGEIKYAGGHSEENIKYQIIQISSLIRLANQLLDECSQILKTETGQDWLFIWDGFE
                     RPEVSPKNVEQLFITYRNVLKELRTNLIFTIPVSLGFAKSAELPFESFLIPDTPVFTR
                     NFGKHIEGRNSLQKVLDSRINPSLFEPHQSERLMVASGGNLRDLFKLVSEAANLALDR
                     SSSPAQIKESDVTKAMNKLRAEYRTRLGDDLQESTHIPYSEKAAKLMQIYKQKPDDQV
                     PNAVLYFLLRARAVQEFNNGDRWFGIHPLVVDILAEQRRIRPVNGVIRGGTK"
BASE COUNT          330 a          276 c          268 g          356 t
ORIGIN      
        1 gggcgcattg cgcccccagc gttctagccg tcgggtgaca aattcaattg tcccatcttg
       61 aagattctca cttggtacca cctcgaatca ccccattgac aggtcgaatc ctgcgttgtt
      121 cggccaaaat atcaaccacc agtggatgaa ttccaaacca gcggtcacca ttgttaaatt
      181 cctggactgc ccgggcgcgc aacaaaaaat agagcacagc atttggaacc tgatcatcgg
      241 gcttttgctt ataaatctgc attaatttgg ctgctttttc agagtagggg atatgagtgc
      301 tttcctgaag gtcatctcca agtcgggtgc gatactcagc acgcaattta ttcatcgctt
      361 tcgtcacgtc agattctttg atctgggctg gtgaagaaga tcggtccagc gccaaattgg
      421 ctgcttcgga taccagtttg aacaaatcac gaagattgcc gcccgatgca accatcagac
      481 gttcgctttg atgaggttca aaaagactgg gattgattcg ggaatccaaa accttctgaa
      541 gtgaattgcg tccttcaatg tgtttcccaa aattccgggt gaaaacagga gtatctggaa
      601 tgaggaaact ctcaaaaggg agttcagcgc ttttcgcaaa tcctaagctg actggaattg
      661 taaaaatgag gttcgtccgg agttctttta agacattgcg gtaagtaatg aaaagttgct
      721 ctacattttt gggcgaaacc tcagggcgct caaatccatc ccagataaaa agccaatctt
      781 gaccagtttc agttttcaaa atttggctac actcatcaag aagctgattg gcgagtcgaa
      841 taagagatga aatctgaatg atctgatatt tgatattctc ttcgctatgt ccgccagcat
      901 atttaatctc accttttatt gcggcaatga gccccaaaaa gttggccaga aggcttttat
      961 cgtctgcccc tgcaccggct gagatttctg ctccaaacat tgttgttcgg ttttctttct
     1021 gggtttttgt tgtaaaccaa tctttaatct ctcccagtaa ctgagaagaa ggtgatttac
     1081 ctgttttctc cttcgttgct tcaaccacct ggatcatcat taaataaatg atatcaaagg
     1141 gctgaaacac agccggattg agatctgttg aggcgttaaa gctaatggtt tgaaactttc
     1201 cagctatttt actggaaagt ctgactaact
//
LOCUS       DAOQDO010002289         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144242_length_1229_cov_3.694208, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002289 DAOQDO010000000
VERSION     DAOQDO010002289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144242_length_1229_cov_3.694208"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKZ53_26700"
     CDS             complement(<1..1074)
                     /locus_tag="PKZ53_26700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44102.1"
                     /translation="MKYKPSPILIVLGLLMALLGILATLQYRWIGQVTESERTRLQAS
                     LQSDIAHLAEDFDREITRIFLAFQMEAVPLNRQAWNAYAERYHQWSVTSPYPDLVDQV
                     YVVNANSRGRLNLVRFDSSTQTFVKDHWPEDLSELRRTLRQQLRQTKSKSGHVFSYNV
                     PSIVETIPALVIPLSNLQFTPDVQGFTVSSLTGYTIVKLNQRTIQRELIPSLIRNDLH
                     NSEQNGYEVVVFSRKNPQTIVYQTSSAKLPGLEQGDASANILSVQVQKFGHLLRESQL
                     LINSVKKKDTATPKTQISVNVMNQVSGEVFVRAMPPISDETGYWRLVMRHQSGSLEQM
                     ITQTRRRNLAISFGILVLLGISVA"
BASE COUNT          304 a          304 c          314 g          307 t
ORIGIN      
        1 ggcgacgctg atgccaagca acaccaaaat gccaaaactg atggccaggt tgcggcggcg
       61 ggtctgggta atcatttgct caagcgatcc ggattgatgg cgcatcacca gccgccaata
      121 gccggtttca tcactgattg gtggcattgc cctgacaaag acttcacccg agacctgatt
      181 catcacattg actgaaatct gggtctttgg cgtggcggtg tccttcttct tgacgctgtt
      241 gatcaataac tggctttccc gcaacaaatg tccgaacttc tgaacctgga cactcaaaat
      301 attggcgctg gcatcaccct gctcaaggcc aggcaatttt gctgatgagg tctggtagac
      361 aatcgtctgg gggttcttgc ggctaaacac tacgacttca tagccattct gctcgctgtt
      421 atgcaaatcg tttcggatca atgacggaat cagttcgcgc tggatggtac gctgattgag
      481 tttgacaatg gtgtatcccg tcaatgatga aacggtaaaa ccctggacgt caggtgtgaa
      541 ttgcaaattg gaaaggggaa tcaccagtgc cggaatcgtt tcaacaatcg aggggacgtt
      601 atatgaaaag acgtgcccgg actttgattt ggtttgtctc aattgctggc gcaacgtccg
      661 gcgtaactcg cttaaatcct ctggccagtg atctttgacg aaggtctgag tggatgaatc
      721 aaagcggacc agattgagtc gcccacgtga attggcattg accacataga cctgatcaac
      781 cagatctgga taaggtgagg tcacgctcca ctgatgatac cgttcggcat aagcgttcca
      841 ggcttgccgg ttcagtggaa cggcttccat ctgaaatgcc agaaaaatac gggtgatttc
      901 ccgatcaaaa tcctcagcca gatgggcgat atcactctgc aagctggcct gcaggcgagt
      961 tcgttcgctt tcagtaacct ggccaatcca gcgatattga agggttgcca gaatccccag
     1021 cagggccatg agcaatccca ggacaatcag aattggcgac ggtttgtatt tcataatgca
     1081 accgctttac gtcaggagtc agtaacggac tttgtttgtg tgaagcaatc acgtcactct
     1141 ttgcaacgac gcgcgaccat cgtggcattg agcctgtata tattacctca agaccaaccc
     1201 ggtctttcat tccagggcct gatactatc
//
LOCUS       DAOQDO010002290         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144749_length_1227_cov_2.551195, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002290 DAOQDO010000000
VERSION     DAOQDO010002290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144749_length_1227_cov_2.551195"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="flgK"
                     /locus_tag="PKZ53_26705"
     CDS             complement(<1..794)
                     /gene="flgK"
                     /locus_tag="PKZ53_26705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNJ44103.1"
                     /translation="MGIHFSLYNIGQSALRTSQLGLATTGHNIANVNTPGFSRQEVQQ
                     SASPGIGTQPLLAGNGVTIDGVRSMRDSFIETRLVTETGVSGRLTAQRDALLPVEAAF
                     NETSGGGISAAISNFFGAFRDLEAHPTSLPLRTSVVQAGQQLGAAFGRTASRLGDIQT
                     QANSLLVSTVAQVNTLSDKIAGLNAKIQVAQSTGGATSELVDSRNQATNELAELVGVK
                     PSSNQDGTLTLTLADGHPLVIGNQAARLVATPTPPAGLASLTVGGKP"
     gene            complement(798..>1227)
                     /locus_tag="PKZ53_26710"
     CDS             complement(798..>1227)
                     /locus_tag="PKZ53_26710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44104.1"
                     /translation="EMIRRTTELMREQTHSYHRLTSNCEQLIVALVRGNPEQIENLTR
                     TGERELLTIRSRLLQITSTLTTFAQARTSAPNRAPISPETRRSFEVASQALIQAAQAF
                     QLVRQRSASLAANGTVFAHACLEACGGLATRYTSSYGKRR"
BASE COUNT          251 a          333 c          373 g          270 t
ORIGIN      
        1 ggtttgccgc cgactgtcaa cgacgccaat ccggcaggcg gggttggggt ggcgaccagc
       61 cgggcggcct gatttccaat caccagcggg tgaccatccg ccagggtgag cgtgagtgtc
      121 ccatcctggt tcgaactggg ttttactccg accagttcgg ccagttcatt tgtcgcctga
      181 ttgcggctgt caaccaactc agaggttgcc ccaccagttg attgagcgac ctggattttg
      241 gcattgaggc cggcaatttt gtctgagagc gtgttgacct gagcgacagt tgaaaccagc
      301 agggaatttg cctgggtttg aatatcgccc agtcgcgatg ctgtccggcc aaaggcagca
      361 cccagttgct gaccggcctg gacaaccgac gtgcggagcg ggagcgatgt cggatgggct
      421 tcgaggtcac gaaaggcacc aaaaaaattg gagatcgccg cgctgatacc accgccgctg
      481 gtctcattaa atgcggcttc aaccggaagc aaggcgtcac gttgggccgt cagccgacca
      541 gaaacacctg tttcggtcac cagtcgggtt tcaataaaac tgtcccgcat cgaacgcacc
      601 ccatcaatgg tgacgccgtt tccagccagc aacggctgtg tcccaatccc tggtgaggct
      661 gattgttgaa cttcctggcg cgaaaagccg ggggtgttga cgttggcaat attatgaccg
      721 gtggtggcca gcccaagctg gctggtccgc aaggcacttt gtccaatatt gtagagtgaa
      781 aaatggattc ccatacctca cctccgtttg ccatacgaag atgtataccg ggtggccagt
      841 cccccgcacg cttccaggca ggcatgggca aacacggttc cattggccgc cagtgaggca
      901 gatcgctgac gcaccagttg aaaggcctgg gctgcctgga tcaacgcctg tgaggcgact
      961 tcaaaactgc gccgggtttc aggtgagatc ggggcacgat tgggcgcact ggttcgggcc
     1021 tgagcaaacg tggtcagggt cgaagtaatc tgcagcagtc gagatcggat ggtgagcaat
     1081 tcccgctcgc cggttcgcgt caggttttca atttgttctg ggttgccacg aaccagagcc
     1141 acaatcagtt gctcacagtt gctggtcagg cggtggtagg aatgtgtctg ttctcgcatc
     1201 agttcggttg tgcgtcgaat catttct
//
LOCUS       DAOQDO010002291         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144804_length_1227_cov_2.147611, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002291 DAOQDO010000000
VERSION     DAOQDO010002291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144804_length_1227_cov_2.147611"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..>1227
                     /locus_tag="PKZ53_26715"
     CDS             57..>1227
                     /locus_tag="PKZ53_26715"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44105.1"
                     /translation="MPLFEKVADKNNLATALNNLGLAYSALGEHPKALGFLTGALPIL
                     QELGLQKEVATTLNNLGTVYNQLGQQTKALELYQQALPIFRTIGDQKGEAFTLNNIGV
                     IFQQTGELKKALDYFQQALPLRRSSGDRRGEAATLSNVGLTYNYLGEFWKSLDHYAQA
                     LVLMKAIGDRRSEATVLVNLAQAYSTLGNPEKSLVSLLDALALFQKLGDRSLEATTFN
                     NIGVAYVLQGKLQKALEQYEPALQLRQQVGDKNGAAATLNNIGDVYFSLGEYPKALDS
                     YFKALSLKRETGDQRGIASAYAGIGYAWFMLDEPSKAAGYLNQALAIFEKAGDRRQTA
                     SLLCDLGQAERKLGNFAQAQSNFERGLSLIESVRFEIKNTDLQATYLATQQDIYSR"
BASE COUNT          340 a          290 c          315 g          282 t
ORIGIN      
        1 ttgtgtactc gtcaaccggg caagaaaaga cagcaatcac atattacctg gaatcggtgc
       61 ctctgtttga aaaggtggcc gacaaaaata acctggccac cgcgctcaac aatctggggc
      121 tggcctattc ggcactcgga gagcatccga aagctctggg atttctgaca ggggcattgc
      181 ctatccttca ggaactggga ctgcaaaagg aagttgctac aacgcttaat aatttgggga
      241 cagtgtataa ccagttgggg caacaaacca aagccctgga attgtaccag caggcgctcc
      301 ccatttttcg cacaattgga gatcagaaag gtgaagcctt taccttaaat aacatcggcg
      361 tgatctttca gcaaacgggt gaattaaaaa aggccctgga ctatttccag caagccttac
      421 cattgcggcg ctcaagtggg gaccgacgag gtgaagcggc aaccttgagc aatgtagggt
      481 tgacctacaa ttatttgggc gaattctgga aatcactcga ccactatgct caagccctgg
      541 ttttgatgaa ggccattgga gacaggcgga gcgaagccac cgtgctggtg aatctggcgc
      601 aagcctattc aactctgggc aatccagaaa aatcgttggt atcgttgctt gatgcgcttg
      661 ccttattcca aaaattggga gaccgctccc tggaagcgac caccttcaac aacatcggag
      721 ttgcctatgt gcttcaggga aaacttcaaa aagccctgga gcagtatgaa ccggcactcc
      781 agttgagaca gcaagttggt gataagaatg gcgcggctgc cacgctcaac aatatcgggg
      841 atgtgtactt cagtttagga gagtacccga aagcacttga ttcctatttc aaagctttgt
      901 cactgaaacg tgaaacaggt gaccagaggg gaatcgcttc ggcttatgcc gggatcggat
      961 atgcctggtt tatgttggat gagccgtcaa aagcggcggg ctatctcaat caggcactgg
     1021 ccatttttga aaaagccggt gaccggcgtc aaacagccag cctgttgtgt gatcttgggc
     1081 aagctgaacg aaaacttgga aattttgctc aggcgcagtc aaactttgaa agaggattaa
     1141 gtcttatcga atctgtccgg ttcgaaatca aaaacaccga cctgcaagcc acctaccttg
     1201 ccacacagca ggatatttat tccagat
//
LOCUS       DAOQDO010002292         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144881_length_1227_cov_1.693686, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002292 DAOQDO010000000
VERSION     DAOQDO010002292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144881_length_1227_cov_1.693686"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..>1227)
                     /locus_tag="PKZ53_26720"
     CDS             complement(150..>1227)
                     /locus_tag="PKZ53_26720"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HNJ44106.1"
                     /translation="PPIQVDGYDLGQIQTEISNVLGRYRNTTWWVNLTCGSKVMSLGA
                     YEAAKLHPGTSVWYLDSNHRQVISLQGNPPGGDVFRLTVSEYLTAYRRMPMKTSDDPF
                     FPKKLALAELFFNQSEKIAVLKKLLGSQNAESGTQDQYSTLKLPTSDRWIIQACQMAK
                     EANLIQSLSSGFEFLTLKVIGHDFWKFVNGTWFEFIVWNAARKAGCFDDVKMDVEIPG
                     KFGLNQLDLAATFSANLLIAECKTDANPFRSKASKERLAEQQKTNPGYRPDPEKIEES
                     GTAYLDKLNSVSSLIGGSFVTKVFICTANPPNPQKNPGLYKTYEQFCSQAVERQIVIV
                     TGDQLHQLPAILHREGKNPTYPRT"
BASE COUNT          294 a          289 c          305 g          339 t
ORIGIN      
        1 aatactggct cccagatgcc acatcaaacc caggagacag gccaggttca ggtgatggaa
       61 tgggagaccc cgaccgggag cttctttgaa acaaagtcca ttccgacgca ggaatggatc
      121 caccggagtt ttgtggcaaa gtgaaccgat caggtccgcg gataggtcgg gtttttacct
      181 tctcgatgaa gaattgccgg aagctggtgc aattgatccc cggttactat gacgatttgg
      241 cgttcaaccg cctgactgca aaactgttcg tacgttttat agaggccggg attcttctgg
      301 ggatttggtg gattggctgt acaaatgaac actttggtca caaagcttcc tccgattaaa
      361 ctggagacag aattgagttt atccagatat gccgttccag attcttctat tttttcaggg
      421 tcgggcctgt agccggggtt tgttttttgt tgttcggcga gtcgttcttt ggaggctttg
      481 gatcgaaaag ggtttgcatc tgttttgcat tctgcaatca ataagttggc tgaaaacgtg
      541 gccgccagat ccagctggtt caaaccgaat ttccctggga tttcaacatc cattttgaca
      601 tcgtcaaaac aacccgcttt gcgagcggca ttccagacga taaattcaaa ccaggtaccg
      661 tttacaaact tccaaaaatc gtgaccaatc actttcagcg tcaggaattc gaacccgctc
      721 gaaaggcttt gaatcaggtt ggcctccttt gccatttggc acgcctgaat gatccatcgg
      781 tcactggttg ggagtttgag cgtagaatat tgatcttgag ttccgctctc agcattctgg
      841 cttcccaaaa gttttttcaa gacggctatt ttttcactct gattaaaaaa caactcagcc
      901 agtgccagtt ttttcgggaa gaaagggtcg tcagatgttt tcatgggcat gcgccggtag
      961 gccgtcaggt attcgctcac tgtcaaccga aagacatcac cgcccggggg atttccttgc
     1021 aaggagatga cctggcggtg attggaatcg aggtaccaaa ccgaagtgcc gggatgtaat
     1081 tttgctgctt cataggcccc caaactcatc actttgctac cgcaggtaag gtttacccac
     1141 catgttgtat ttcggtaacg tcccagaaca ttgctgattt cagtttgaat ttgccccaga
     1201 tcatagccat caacctggat cggcggg
//
LOCUS       DAOQDO010002293         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_144938_length_1226_cov_3.796755, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002293 DAOQDO010000000
VERSION     DAOQDO010002293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144938_length_1226_cov_3.796755"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..919)
                     /locus_tag="PKZ53_26725"
     CDS             complement(125..919)
                     /locus_tag="PKZ53_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNJ44107.1"
                     /translation="MLIAAVQLRSTDQVESNLNRVASLVAEAAHRGAALVALPENFAY
                     LRSEGEDIGYHQSLDGELADWLKQLAQKHGISLLAGSFPETAPNQKVFNTSLLFGPDG
                     QQLALYRKVHLFDVTLPDGTVLEESRFVEPGQELVTVTIDDVCFGLSICYDLRFPELY
                     RQLTAQGAQVLFIPAAFTLQTGKDHWEILLRARAIENQVYVVAPAQFGQHTPKRASYG
                     RAMIIDPWGMVLATAPDRECFIMAEIDTSHLARIRKAMPCLLHRRM"
BASE COUNT          273 a          307 c          340 g          306 t
ORIGIN      
        1 gttccgggtt ccgggttccg ggttccgggt tccgggttcc gggttttcga aaccccatct
       61 tttttgtcct tttcgtcctt ttcgtccctt gtcttttcta atccccggaa cccggatttg
      121 gcccctacat tcggcggtgc aacaaacagg gcattgcttt gcgaatgcgg gcaaggtggc
      181 tggtgtcaat ttcggccatg atgaagcatt cgcgatctgg cgccgtcgcc agaaccatcc
      241 cccaggggtc aataatcatg gctcgtccat aactggcgcg tttcggagtg tgctggccaa
      301 attgagctgg agcaacgaca tacacctgat tttcaattgc gcgggcccgc aacaaaattt
      361 cccagtggtc ttttccagtt tggagggtaa acgcggctgg aatgaataaa acctgtgctc
      421 cctgggccgt gagctggcgg tagagttcag gaaagcgcag atcgtaacag atggaaagtc
      481 caaaacagac atcatcaatc gttaccgtca ccagttcctg acctggttca acaaagcgag
      541 attcttcgag cacggttcca tcgggcaagg tgacatcaaa aagatgcact ttccgataca
      601 acgccagttg ttggccatct ggcccaaaga gcagactggt gttaaagact ttctgattgg
      661 gagccgtttc aggaaaactc ccagccagca gggaaatacc gtgtttttgg gccagttgtt
      721 tgagccagtc ggccagttct ccgtcaagcg actggtgata gccgatgtct tcgccttcag
      781 agcgcaaata ggcaaagttt tccggaagcg cgaccagcgc ggcaccccgg tgggcagctt
      841 cggccaccag tgacgccacc cggttgagat tggattcgac ctgatcagtt gatctgagtt
      901 ggaccgccgc aatgagcata attcccccaa tttggtgcct ggtgcctggt gtttagtcgt
      961 accacgaggt tttattcccg taggttaaga ggctgtaatc ggcgtctacg atgtgaaatt
     1021 ctcgcagcca tggtattgcc tgataataaa ccacccagtc aagggaggtc tgggcatcaa
     1081 attccggtcg ggataaaaac gccgtgagca agagaagatt ggattgagga tcaagagagg
     1141 atttttttca ggctcggtca atggcattaa gttaaggtcg gagcgccggt atcttgcccc
     1201 tgctgctacc ggattttgtg ttggag
//
LOCUS       DAOQDO010002294         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_145008_length_1226_cov_2.436379, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002294 DAOQDO010000000
VERSION     DAOQDO010002294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145008_length_1226_cov_2.436379"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKZ53_26730"
     CDS             complement(<1..422)
                     /locus_tag="PKZ53_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNJ44108.1"
                     /translation="MRREETLRCSFCGKTQNEVKKLIAGPTVYICNECIDICNEIISD
                     DAHQEATAQRPQLPKPVEVKAFLDDYVIGQDETKKKLAVAVYQHYKRMDMLKRRTSGD
                     VEIQKSNILLIGPTGTGKTLLAQTLARMLNVPFTIVDAT"
     gene            complement(548..1147)
                     /gene="clpP"
                     /locus_tag="PKZ53_26735"
     CDS             complement(548..1147)
                     /gene="clpP"
                     /locus_tag="PKZ53_26735"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNJ44109.1"
                     /translation="MITPVQTLVPMVIEQTGRGERAYDIYSRLLKDNIIFIGTPIDDM
                     IANLVVAQLLFLEAEDPDKDISLYINSPGGSITAGMAIYDTMQFIRPDVTTICVGQCA
                     SMGALLLAAGAKGKRFALPNSRILIHQPSGGAQGQATDIRIQAEEILRMRELTSNILS
                     RHTGQPFDVIERDVERDRIMSAVQAKEYGLIDQVLAHRE"
BASE COUNT          299 a          299 c          338 g          290 t
ORIGIN      
        1 gtggcatcaa cgatagtgaa gggcacattg agcatccggg ccagggtttg ggcgagcagt
       61 gttttgccag tgccagttgg cccaatcaac aagatgttgc ttttttggat ttcaacatcg
      121 cccgaggtcc ggcgcttgag catgtccatc cgcttgtagt gctgatagac ggccaccgcc
      181 agttttttct tggtttcatc ctgcccaatg acatagtcat caagaaaggc tttgacctcg
      241 accggcttgg gaagctgggg acgctgggct gtggcctcct gatgggcatc atcagaaatg
      301 atttcgttgc agatatcaat acattcattg cagatataga cggtgggccc ggcgatgagt
      361 tttttgactt cattctgggt ttttccacaa aaagaacagc ggagtgtctc ttctcttctc
      421 acacgtagct ccagggggaa gaataataat taatgaaccg tgaccgtcgg gtatggcgtc
      481 acaacgccca cccgaatggc ccctggctgg caatcaaccg ggggccgcag gggcaagcaa
      541 gaactgacta ttcgcgatgg gctaaaacct gatcaatcaa gccgtattct ttggcctgga
      601 cggctgacat aatccggtcg cgttcaacgt cgcgttcaat cacatcgaag ggctgtccgg
      661 tgtgcctgga caaaatgttg gaggtgagtt cccgcatccg caggatttct tcagcctgaa
      721 tccggatatc ggtggcctgg ccctgggcgc cacccgatgg ttggtgaatc aggatgcgcg
      781 agttgggcag ggcaaaccgt ttgcctttcg ctccggcagc cagcaacaac gcacccatcg
      841 aagcacactg gccaacgcaa atcgtggtca catcgggccg aatgaactgc atggtgtcgt
      901 aaatggccat tcctgccgtg atcgagccac cagggctgtt gatatagagc gaaatatcct
      961 tgtccggatc ctcggcttcc aaaaacaaaa gttgcgcgac caccagattg gcgatcatgt
     1021 cgtcaattgg ggtaccgatg aaaataatgt tgtctttcaa caaccgggag taaatgtcat
     1081 aagcacgttc accacgaccg gtttgctcaa tgaccatggg aacgagggtt tgtacagggg
     1141 tgatcatgga cgtctaccta aatcaaatag tgaatggggt ggagaaccaa aatatccaaa
     1201 ttatgaggta tgaagtatga agtatg
//
LOCUS       DAOQDO010002295         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_145185_length_1225_cov_3.447009, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002295 DAOQDO010000000
VERSION     DAOQDO010002295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145185_length_1225_cov_3.447009"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1225)
                     /locus_tag="PKZ53_26740"
     CDS             complement(<1..>1225)
                     /locus_tag="PKZ53_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNJ44110.1"
                     /translation="GQLPAYEWAFDDVNPPVHAWAAWRVYKIDKRIRGVGDRRFLERV
                     FQKLLLNFTWWINRKDAEGNNVFQGGFLGLDNIGVFDRSAVLPTGGHLEQSDATSWMG
                     MYCLNMLAIALELAHDNMAYEDVASKFYEHFLYIANAMNHISNEDIELWSEKDGFFYD
                     VLQLPNGSHFKLRVRSMVGLIPLFAAQTMEADWLKQLPGFRKRMDWFVKNRPELQRNI
                     SSVDEEGNIVQQLLSIVNASQLRRVLKVMLNEAEFLSDHGIRSVSRFHLTDPYVLQIG
                     GQEYRVDYDPAESSTGLFGGNSNWRGPIWFPVNYLIIESIQKFHHFFGDDFKVEFPTG
                     SGNHLNLWEVSVELSRRLTRIFLKDENGRRPVYDMPEMEKFQTDPNWQDLVLFYEYFH
                     GDSGRGIGASHQTGWT"
BASE COUNT          294 a          321 c          326 g          284 t
ORIGIN      
        1 gtccaacccg tctggtggct ggcaccaatg ccacgaccgc tgtcgccgtg gaagtattca
       61 taaaacaaaa ccagatcctg ccagttgggg tcggtttgga atttttccat ttccggcatg
      121 tcgtacactg gtcgccggcc attttcatct ttcaggaaga tgcgtgtcag acgacgggaa
      181 agctcaaccg agacttccca caggttgagg tgattgcccg aaccggttgg aaattcgact
      241 ttgaaatcat ctccaaaaaa gtggtggaac ttctggatgg attcaatgat gagatagttg
      301 actgggaacc agattggccc acgccagtta gagttcccac caaacagacc ggtgctggat
      361 tcagccggat catagtcaac tcgatattcc tgaccgccga tctgaagcac atacgggtct
      421 gttaagtgaa accgcgaaac agagcggatg ccatgatcag agaggaattc cgcttcgttg
      481 agcatcacct tgagcactcg tcgcagttga ctggcgttca cgatggacag caattgctgg
      541 acgatattgc cttcttcatc aaccgaagaa atattgcgct gcagttccgg acgatttttg
      601 acaaaccagt ccatgcgttt gcggaagcct ggaagttgtt tgagccagtc agcttccatc
      661 gtttgcgccg caaacagcgg aatcaggcca accatggatc ggacccgaag cttaaagtgg
      721 ctgccgttag gaagctggag cacatcataa aagaacccat ctttttcgct ccacaactca
      781 atgtcttcat tgctgatgtg gttcatggca ttggcgatat agaggaagtg ctcataaaac
      841 ttgctggcga cgtcttcata ggccatgttg tcgtgcgcca gttcgagggc aattgccagc
      901 atgttgaggc agtacatgcc catccagctt gtggcgtctg actgttcgag gtggccaccc
      961 gtcggcaaaa cggcgctccg gtcaaaaacg ccgatgttgt cgaggcccag aaaaccgcct
     1021 tgaaagacgt tgttgccttc ggcgtccttg cggttgatcc accaggtgaa gttcaacaga
     1081 agtttctgga acacccgttc cagaaagcgc cggtcgccaa cgccgcgaat ccgcttgtcg
     1141 atcttgtaca cgcgccaggc ggcccaggcg tggacgggcg ggttgacatc gtcaaaagcc
     1201 cattcatacg ccggtaattg cccat
//
LOCUS       DAOQDO010002296         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_145751_length_1223_cov_2.308219, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002296 DAOQDO010000000
VERSION     DAOQDO010002296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145751_length_1223_cov_2.308219"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKZ53_26745"
     CDS             <1..361
                     /locus_tag="PKZ53_26745"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44111.1"
                     /translation="DMEFSKHAGGVLAVACSADGKLAASAGQDTTIQVWDLQTGAVIQ
                     TLKGHSDWINSLAFGGDPRRLISASIREESIRIWDLESGQLLLPLKGKSVALSPDGAL
                     LAIADDEGIRTFPVNFK"
     gene            374..868
                     /locus_tag="PKZ53_26750"
     CDS             374..868
                     /locus_tag="PKZ53_26750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44112.1"
                     /translation="MKYSLLLSVILLGSWIPTTGVTSQNRKKPAAIARHKVTDEGQAA
                     KSGTQVDPNIQVAGPLNPDNPAQSRPAPPQKGGEKPKGGTFSLLQIDNRTRFIINIYV
                     DGVYRGAVDAWGNSEGFLDSGEHRVYGKAQLDSGTTVTWGPRVIFLSSGDQTRWTLTP
                     NSGK"
     gene            924..>1223
                     /locus_tag="PKZ53_26755"
     CDS             924..>1223
                     /locus_tag="PKZ53_26755"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ44113.1"
                     /translation="MFTKLVVVSLVSFLIVFTSLGYTSARQSPQPTQNPPETIAEQNR
                     KIIQAALEKSRIQTEGENGKPRLMVQLGHSGPVTSVAFSPDGQFVLTGSADNTARV"
BASE COUNT          308 a          317 c          334 g          264 t
ORIGIN      
        1 agatatggaa ttcagtaaac acgcgggtgg tgtgctggcg gtggcttgtt cagccgacgg
       61 caaacttgct gcttcagccg gacaagacac gacaattcag gtgtgggatt tacaaaccgg
      121 tgctgtcatt cagaccctca agggacacag tgactggatc aactccctgg cttttggtgg
      181 tgacccccgg cggttgattt ccgccagcat tcgcgaggag tcgatccgca tttgggatct
      241 ggaatcgggc cagttattgc taccgctcaa agggaaaagc gtcgcacttt cacctgacgg
      301 tgcacttctc gccattgccg acgacgaggg aatccgcacg ttcccggtca attttaagta
      361 ggggggaaac cgcatgaagt attcactgtt gctcagcgtc attcttttgg gaagctggat
      421 cccaaccact ggagtcacca gccagaatcg aaaaaaacca gccgccatcg cgcgccacaa
      481 ggtaaccgat gaagggcagg cggcaaaatc aggtacccag gtagatccaa acatccaggt
      541 tgccggacca ctcaacccgg ataatccagc ccagagtcgt ccggcaccgc ctcaaaaggg
      601 tggcgagaaa ccaaagggag gaaccttcag tttactgcag attgacaacc gtacccgctt
      661 tatcatcaat atttacgttg atggcgtata ccggggagca gttgatgcct ggggaaattc
      721 agaagggttt ctcgattccg gagagcatcg agtgtacggg aaagcccagc ttgattccgg
      781 taccaccgtt acctggggac cacgagtgat cttcctttcc agtggagacc agaccagatg
      841 gacgttgact cccaattccg gaaagtaagt tgcaaacctt ggctgaacca cttgtaaacc
      901 gcacttttga aggagaataa gcaatgttta ccaagctggt agttgtttca ctggtgtcct
      961 tcctgattgt gtttacgtcg ctgggctaca ctagcgcgag gcagtctccc caacccaccc
     1021 agaatccacc agaaaccatt gctgagcaga accgcaaaat catccaggca gcccttgaga
     1081 aaagccgaat ccagacggaa ggcgaaaatg gcaaaccacg gttgatggtg cagttagggc
     1141 attcgggtcc tgtgactagc gtggcctttt cgccggatgg gcagtttgtg ttaactggaa
     1201 gtgccgataa tacagctcgg gtg
//
LOCUS       DAOQDO010002297         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_145898_length_1222_cov_4.490146, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002297 DAOQDO010000000
VERSION     DAOQDO010002297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145898_length_1222_cov_4.490146"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKZ53_26760"
     CDS             <1..504
                     /locus_tag="PKZ53_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNJ44114.1"
                     /translation="LLVVQECRAIPLPLESTAKQIPDHCGSCTACLDACPTEAIVQPY
                     VVDARKCISFATIESKEDSLNLPTEGWIFGCDICQDVCPWSRFSRPTSESRFSPHEDF
                     QAPDLAQWQQLTPEDFARKFTGSPIKRAKYRGLQRNIAHVQLQSRLSEIEPDAPQPVT
                     GCASEAE"
     gene            523..>1222
                     /locus_tag="PKZ53_26765"
     CDS             523..>1222
                     /locus_tag="PKZ53_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ44115.1"
                     /translation="MGGNPSVSQARTLKHQPLQTKDATMTQGDIFVIDDNPINLNLLA
                     HLLRERGYQVRIATSGRRGIVAVRTSVPDLIMLDITMPEMNGYEVCQQLKADDQLRDI
                     PVIFISALDDALDKVKAFEVGGVDYVTKPFQIEEVLARIENQLRISQLQKELRETNNQ
                     LERQRAQLLEANQKLKDVEQAKADFTAMLVHDLKSPLTVVKTMLDLLQFDEVINQAIA
                     EQRLTDVMVASERNI"
BASE COUNT          314 a          335 c          304 g          269 t
ORIGIN      
        1 ctgcttgtcg ttcaggaatg cagagcgatt ccactgccgc tggaaagcac cgccaaacag
       61 attccagacc actgtggaag ctgcactgcc tgcctcgatg cctgcccaac tgaagcaatt
      121 gtccagccct atgtggtgga cgcccgaaag tgcatttcat ttgccacgat tgaatcgaag
      181 gaagattcct tgaatcttcc taccgaaggc tggatttttg ggtgtgatat ctgtcaagat
      241 gtgtgcccgt ggtcgcgatt ctcccgacca acctccgaat ctcgcttttc cccgcacgaa
      301 gattttcagg cacctgacct ggctcagtgg caacaactga caccggagga ttttgcccgc
      361 aagtttaccg gatcgccgat caaacgtgcg aagtaccggg ggctgcagcg taatattgcc
      421 cacgtacaat tacagtcacg attgagcgaa atagaaccgg atgctcccca gccggttact
      481 ggttgtgcct cagaagctga atagaaggaa ggttggcccc acatgggagg aaatccatct
      541 gtttcccagg cccgaacgct gaaacatcaa ccacttcaga ccaaggacgc gacaatgact
      601 caaggcgata tctttgtcat cgacgacaac ccaatcaatc tcaatctgct cgcccacctc
      661 ctgcgagaac gtggctatca ggttcggatt gcgaccagtg gtcggcgtgg gattgtggca
      721 gtccgcacgt cagttccgga cttgatcatg ctcgatatca ccatgccgga aatgaacggg
      781 tatgaagtct gtcagcaact gaaagctgat gaccagctcc gtgatattcc agtcattttt
      841 atcagtgccc tcgatgatgc cctcgataaa gtcaaagcct ttgaagtcgg tggggttgac
      901 tatgtgacca aaccctttca aatcgaggaa gtcctggccc gaattgaaaa tcagttgcga
      961 atttcacagc tccagaaaga gctacgcgaa accaataacc agctcgaacg ccagcgagcc
     1021 cagttgcttg aagccaatca gaaactcaaa gatgtggagc aggccaaagc cgattttacg
     1081 gcgatgctgg ttcacgactt gaaatcgccg ctcaccgtgg tcaaaaccat gctggacttg
     1141 ctccagtttg acgaagtcat taaccaggcc attgccgaac aacgactcac cgacgtcatg
     1201 gtggcatcgg agcgcaacat ag
//
LOCUS       DAOQDO010002298         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_145907_length_1222_cov_3.619537, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002298 DAOQDO010000000
VERSION     DAOQDO010002298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145907_length_1222_cov_3.619537"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1108
                     /locus_tag="PKZ53_26770"
     CDS             299..1108
                     /locus_tag="PKZ53_26770"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ44116.1"
                     /translation="MSAPDQLNLYSDLQPEPVEPESSTCTKCRGTGWEFLPALRQSRP
                     CSCRKTNQQDTLLTRARIPIRFKNCTLSNYYPQGKPNSPEHTSQARAQQDCHHFLKEY
                     PAIDYGLLFLGPCGVGKTHLSAAVINKLITWKNVPCLFYDFRDLLKEIQGSYNPNTHA
                     TESAVLDPVYKAEVLVLDELGANKPTAWVQDTITQIINTRYNEKKITIITSNYLDTPL
                     PGTKPGEETLTDRVGARLRSRLYEMCKTILVQGEDYRKKVLTYKTQPSLIR"
BASE COUNT          322 a          307 c          288 g          305 t
ORIGIN      
        1 gcctggcaaa agacgagatg cgttcagaaa aatggatact acttgaaaag tgttttcaaa
       61 atcaatgaaa catggtcaag ttttccccag gtattcgggc aaaaaacagg tcaggttttt
      121 gcaggcatgt gctttggaaa acactgtcac cacgcttgat ttttgggcga ccttcgacga
      181 tacttgccga tgctctcacc cgtgagcagc tcgacaaggg ttcagcagtt tactccaaaa
      241 gaaaaacaaa ccggtgtggt ctcagtcacg tgggactggg aatcaccata ggtgtcttat
      301 gtcagctcca gatcagctca atctctattc agacctccag cctgaaccag tagaacctga
      361 gtcgtctacc tgcaccaagt gtcggggtac gggctgggag tttctgccag ctctgcgcca
      421 gtctcgccct tgttcgtgcc gtaagaccaa tcagcaggat actttactca cccgggcccg
      481 gattccaatt cgctttaaga attgcacgct gtccaattat taccctcaag gaaaacccaa
      541 ttccccagag cacacctcac aggctcgcgc ccaacaggat tgtcaccatt tcctaaaaga
      601 gtacccggct attgattacg ggttactgtt tttaggcccc tgtggcgtgg ggaaaaccca
      661 tttgtccgca gcggtgatca acaagttgat tacctggaaa aatgtaccct gcctgtttta
      721 tgactttcgc gatttgttga aagagattca gggaagttat aaccccaata ctcacgcaac
      781 agagtcagcc gtgcttgatc cagtgtacaa agctgaagtg cttgtactgg atgagcttgg
      841 cgccaataag ccaactgcct gggttcagga tacgattacc caaattatca acacccgata
      901 caacgagaag aaaattacca ttatcacttc aaactatctg gacacgccct tgccagggac
      961 gaaacccgga gaagaaacgc tgacagatcg ggttggggcc cgtcttcgat cacggctgta
     1021 tgagatgtgt aaaacgattc tggtccaggg cgaagactac cggaaaaaag tcctgaccta
     1081 taaaacccag ccttcgctca ttcgttgacc ctcattctct tttgcaaatc gaaaggcggc
     1141 tgtgatttat tgtcacagcc gcctttgtgg tgcctggaaa aaaattgtac ttagtgcttg
     1201 gtgcttggtg cttggtgctt gg
//
LOCUS       DAOQDO010002299         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_145925_length_1222_cov_2.944302, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002299 DAOQDO010000000
VERSION     DAOQDO010002299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145925_length_1222_cov_2.944302"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PKZ53_26775"
     CDS             complement(<1..1176)
                     /locus_tag="PKZ53_26775"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ44117.1"
                     /translation="MMKREVTPFSESVLNGPRGVAMDAFGGLYIADTENHTIFYADFL
                     NQELRLIAGRPGQPGKQDGTGKQARFHRPMDLAVSPDGNWLAVADAGNQTIRLVSRIG
                     EVITLDTGPTRLKKIQSLNFDGAGNLYFVDKEGVFVIPFPVGQSLQLVSLAQPERSFN
                     RPTSVVVEGTEAYVFDTDDAITTSTIKLVSVGTPEIISLNQTVAKLEGGTEVLITGRN
                     FAPETRVLVDQTRATLVTVESPSQIRIIIPEQEIPGPYIITVQTRGGLAEAKISIVSK
                     PLSELASGEITTIAGTRKLFPHAGPALSQFIEFLPASILTTDSAGNLFFFDQSRQIRK
                     FNLQSKLVTTILGNGKLHLSDKMDSTIETNLDDFYFVDLDPAGNLYVLSQDHILRVDL
                     "
BASE COUNT          309 a          282 c          305 g          326 t
ORIGIN      
        1 gagatccacc cgaaggatgt ggtcctgact gagcacatac aaattgcctg ccgggtcaag
       61 atcaacaaaa taaaaatcat cgaggttggt ttcaatggtt gaatccatct tatcactaag
      121 gtggagcttt ccatttccaa gaatcgtcgt gacgagttta ctttggaggt tgaactttcg
      181 aatttgccgg ctttgatcaa aaaagaacaa attcccggca ctatcagtgg tcaggataga
      241 ggctgggaga aattcgataa attgactcag ggctgggccg gcatgaggga ataactttcg
      301 agtgccggca atggttgtga tttcccccga cgccagttct gaaagtggct ttgagacaat
      361 tgaaattttg gcttcagcca gacctccgcg agtctgaacc gtgataatat aaggacccgg
      421 aatttcctgt tccgggatga ttattcgaat ttggcttggc gattcaacgg taacgagtgt
      481 ggctctagtt tgatcaacca ggacgcgggt ttcaggtgcg aaatttcgtc cagtaataag
      541 aacttccgtt ccgccttcaa gttttgcaac ggtctgattg agacttatga tttcaggcgt
      601 tcccacactg acgagtttga tggttgaggt tgtgattgca tcatcagtat caaacacata
      661 ggcctcggtt ccttcaacca ccacgcttgt tggccgattg aatgaacgtt caggttgtgc
      721 caacgaaacc agttgcaaag attgacccac tggaaatggt atgacaaata ctccttcttt
      781 atcaacgaaa tagagattac cggccccgtc aaaattaagt gactggattt tttttaaccg
      841 ggttggacca gtgtcaagag ttattacttc acctatccgg gataccagtc gaatagtttg
      901 atttccagca tcagccacgg ccagccagtt gccatctgga ctcaccgcca gatccattgg
      961 gcgatgaaac cgggcctgtt taccagtgcc atcctgcttt ccaggctggc cgggtcgtcc
     1021 ggcgatgagg cgaagttcct gattcagaaa atcagcataa aagatggtat ggttttcagt
     1081 gtcggcaatg tagagcccgc caaaggcatc cattgccacg ccgcgtgggc cgttcagtac
     1141 gctttcagag aaaggcgtga cctcacgttt catcatgcct ggtgagcctg ttccaatcac
     1201 agtggttacc tgatcatcgg tt
//
LOCUS       DAOQDO010002300         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_146000_length_1222_cov_2.183376, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002300 DAOQDO010000000
VERSION     DAOQDO010002300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146000_length_1222_cov_2.183376"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKZ53_26780"
     CDS             complement(<1..446)
                     /locus_tag="PKZ53_26780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNJ44118.1"
                     /translation="MKKQGFRHNHLAVVVMLAACLTWAGYFRTGTTIQANYQVSRLSQ
                     TQLSKIVTIEAPDHHPAFHFRQGQELELTPALETRLSQFQGTPKALAGGDFDSDGMKD
                     VIVGYETATGGTVMVFRGNPETFAPSDARTRKLKAEGTFTESPLLET"
     gene            complement(750..>1222)
                     /locus_tag="PKZ53_26785"
     CDS             complement(750..>1222)
                     /locus_tag="PKZ53_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ44119.1"
                     /translation="MAAIGGLVYLVFPGLTAPPLARSGLMALAGVAWGMYSLRGRGSK
                     TPLADTTSNFVYGVPLAVLVTALMWSQVVITPKGALLALLSGALASGVGYVIWYAALK
                     YLTATRAATVQLAVPVLAAAGGVVVLSEQISMRLIVAALLILGGIGLALFGRKT"
BASE COUNT          308 a          357 c          298 g          259 t
ORIGIN      
        1 gtttccagca gcggcgactc ggtaaacgtc ccttcagctt tgagtttccg ggttctggca
       61 tcacttgggg caaaggtttc tggattccct cgaaacacca taacggtgcc accagtcgct
      121 gtttcatagc cgacaatcac atctttcatc ccgtcggagt caaaatcgcc gcccgccagt
      181 gctttcggcg taccttgaaa ctgactcaat cgggtctcaa gtgccggcgt gagttcgagt
      241 tcctgtccct gacgaaaatg aaaagctgga tgatgatcgg gggcttcaat ggtaacgatc
      301 tttgataatt gggtttgtga taaacgagaa acttgataat tggcttgaat tgtggtgccg
      361 gttctgaaat atccggccca ggtcaggcag gcggccagca ttacgacgac ggccagatga
      421 ttgtgacgaa atccctgttt cttcattttg atacgctctc ctcttgcaga atcaacacgt
      481 tgcagtaagg aggttgtatc agaaaaacca caagacccaa gaaatttgca accagcggtc
      541 aaaaggcgcc accaatggct gaaatcgggg ctcagggctc agggcttggg gctgaagaag
      601 ttgggctgaa gaagttgggt cgaagaagtc aggctgaaga actggttttc tttcatccct
      661 catccttcat ccctcatccc ttcgattgtc ctgagcttgc gagttttcat ccccaagccc
      721 caagccctca gccccaagcc ctcagccctc tatgtcttcc gcccaaacaa agccaaccca
      781 attccaccaa ggatcaatag cgcagccaca atcaaccgca tggaaatctg ttcggaaaga
      841 accacgacac cgcccgccgc tgccagcacc ggtaccgcca gttgcacggt tgcggctcgc
      901 gtggcggtca aatatttcag cgcggcatac cagatgacat acccaactcc agaagccaat
      961 gcaccagaaa gcaatgccag caaagcacct ttgggcgtga taactacctg agaccacatc
     1021 agagcagtga ccagcacggc cagcggcacg ccatacacaa aattactggt tgtgtcagcc
     1081 agcggtgttt ttgaaccacg tcctctgagc gaatacattc cccaggcaac tccggccaga
     1141 gccatcagtc ccgaccgagc cagcggcggc gccgtcaatc ctggaaagac cagatacacc
     1201 agtccgccaa tcgcggccat ca
//
LOCUS       DAOQDO010002301         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_146186_length_1221_cov_3.320755, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002301 DAOQDO010000000
VERSION     DAOQDO010002301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146186_length_1221_cov_3.320755"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKZ53_26790"
     CDS             complement(<1..229)
                     /locus_tag="PKZ53_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44120.1"
                     /translation="MSELMAKDGTTTMARTPRSVKSSASKPVRQEVPEKTRGTSKVKS
                     SKSRAREIIRRLQAEYPTAHCSLEYSSPLELL"
     gene            250..819
                     /locus_tag="PKZ53_26795"
     CDS             250..819
                     /locus_tag="PKZ53_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNJ44121.1"
                     /translation="MPNVLVFLATGFEEMEAVIVVDVLRRAGATVTTAAIGEAQLVTG
                     SHDIAINADVLLNELPSALDSAFDLIILPGGQPGTTNLQHDATVQEWIRTYQAKERGI
                     AAICAAPMALATAGVLKQRTFTGHPGAKSFLETEANGTYTGNRVEIDGKLVTSQSPGT
                     AFEFALALVGELFSQEQAAELGASMLVRA"
BASE COUNT          274 a          295 c          351 g          301 t
ORIGIN      
        1 ccagcaattc cagtgggctg gaatactcaa gcgaacagtg agcggtcgga tattcggcct
       61 gaagccgacg aatgatttcc cgggcgcggc tttttgaaga tttcaccttt gatgtgcccc
      121 tggttttttc aggcacttcc tgccggacgg gctttgaagc tgaggatttg acggaacgtg
      181 gtgtccgcgc catagttgtg gtgccatctt ttgccatgag ttcactcatt atatgaaagg
      241 agacagttta tgccgaatgt tctggttttt ttggcgactg gatttgagga aatggaagct
      301 gtcatcgtgg ttgatgtgtt acgccgggct ggtgccaccg tcaccacggc ggccattggc
      361 gaggcacagc ttgtcaccgg gtcacacgat attgccatca acgctgatgt tttgttgaac
      421 gaactcccgt cagctttaga cagtgcgttt gatctgatca ttctgccggg tggtcaacct
      481 ggaacgacca atttacaaca tgacgccacg gttcaggaat ggattcgcac ctatcaggcc
      541 aaagaacgtg ggattgccgc catttgcgcc gccccgatgg ccctggcgac cgccggtgtg
      601 ctcaagcaac ggacctttac cggccatccc ggagcaaaat catttttaga aaccgaagcc
      661 aacggcacct acactggcaa ccgcgttgaa attgacggga agttggtcac cagccagagc
      721 ccaggcaccg cgtttgaatt tgccctggcg ctggtcggcg aactgtttag ccaggaacaa
      781 gccgctgaac tgggcgcctc gatgctggtc agggcttgat ggggcggaag acttcgggtg
      841 gaagacttca ggctcagggc agaagtcgtt gggctgaaga ttcgcgggct cagggtggaa
      901 gactttgagg ttcgggttga aattccggtt ttcttcagcc tcaggctcgg gttgaggttc
      961 aacctcgggc acctgagatt tttgatattg ttcccagaaa gcgcgaaggt tcgtcaataa
     1021 agtgcgatat cgttgtgagt tccgtgagcg tagcacgtcg tgttggccaa atgatgtttt
     1081 gaagaaaata cagacaattg cataggcaac atcttgaact gacaccacat agcgataggg
     1141 cctgaagtca cgataatagc caactgaacg agttaaccgg gtatctgttt ccaccgtctg
     1201 aatcaaggct tcaattgctg c
//
LOCUS       DAOQDO010002302         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_146660_length_1219_cov_3.284364, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002302 DAOQDO010000000
VERSION     DAOQDO010002302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146660_length_1219_cov_3.284364"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKZ53_26800"
     CDS             complement(<1..201)
                     /locus_tag="PKZ53_26800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44122.1"
                     /translation="MLRKGLLLSFIFLLTACLQVAAQTSAPAKTEGPSVDELLTKSID
                     AMGGAEKFKALKTLKMTGKMIMQ"
     gene            complement(387..1133)
                     /gene="fabG"
                     /locus_tag="PKZ53_26805"
     CDS             complement(387..1133)
                     /gene="fabG"
                     /locus_tag="PKZ53_26805"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNJ44123.1"
                     /translation="MSFQGKTAIVTGGSRGIGRAIVIELARQGCQVAFTYASNAEAAQ
                     QVVNECEAFGVQVKAYQVDAANFEAAQGFVKDVKAQFGAVHYLVNNAGITRDKLILMM
                     KEDDWDAVIDTNLKSVFNLSKAVAALLVRQKQGGSILNISSISGVIGMPGQTNYSASK
                     AGMIGFTKALAKELASREITVNALALGMIETDMTGALDATYREKVTEQIPLKRFGTVT
                     EVARATAFLLSDDGRYITGQVIQIDGGLAM"
BASE COUNT          289 a          308 c          287 g          335 t
ORIGIN      
        1 ttgcataatc attttcccag tcattttcaa tgttttcaat gctttaaact tttctgcgcc
       61 gcccatggca tcaattgatt tggtcaacaa ttcatcaact gaagggcctt ctgttttggc
      121 aggggcactg gtttgggctg ccacctgcaa acaggcggtc agcaaaaata taaaactgag
      181 caacaatcct tttcgaagca tggttaaaaa cctcattcct cactaaatat tggggttaaa
      241 tgtttggtgt cgctgccaat gaacacctgg tgcttggtgc ttggttcttg gttcttggtg
      301 cttggttcct ggtgcttggt tcctggttct tagaaggtat tgatttccag tcaccaagtt
      361 ccaagcacca gtcaccaagt tccaggtcac atggccaatc cgccatcaat ttgaatgacc
      421 tggcccgtga tatatcgccc atcgtcagaa agcagaaaag ctgtcgctcg ggcgacttcg
      481 gtgacggttc caaagcgttt gaggggaatt tgctctgtga ctttttcgcg atatgtagcg
      541 tcgagggcac cagtcatgtc ggtttcgatc atgcccaaag ccagggcatt gaccgttatc
      601 tcacgactgg cgagttcttt ggccagcgct ttggtaaatc caatcattcc ggctttggaa
      661 gctgaatagt tggtttgacc aggcatcccg ataacgccgc tgattgagct gatgttcagg
      721 atactgcctc cctgtttctg tcgaaccagg agggcagcaa cggctttact cagattaaag
      781 acgcttttca ggttggtatc aatcacagcg tcccagtcat cttccttcat catcaaaatc
      841 aatttatcgc gggtaatccc ggcgttgttg accagataat gaacggcacc aaactgggcc
      901 ttgacgtctt tgacaaaacc ctgggcggct tcaaaattgg cggcatcaac ctgataggct
      961 ttgacttgca cgccaaaagc ttcgcattca ttgacgactt gctgggcagc ctctgcattt
     1021 gaagcgtagg taaaagccac ctggcacccc tgacgagcca gttcgatgac gattgcgcgg
     1081 ccaatccccc gtgaaccacc ggttacaatt gctgtttttc cttgaaatga catgacactg
     1141 attccttctt aaagtcagta ccacctgcgt cagcgggtgg cattaaaaaa tgagagccac
     1201 ccaactcaca caggtggca
//
LOCUS       DAOQDO010002303         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_146842_length_1219_cov_1.620275, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002303 DAOQDO010000000
VERSION     DAOQDO010002303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146842_length_1219_cov_1.620275"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..>1219)
                     /locus_tag="PKZ53_26810"
     CDS             complement(352..>1219)
                     /locus_tag="PKZ53_26810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44124.1"
                     /translation="SFTDPLTAKKLRAMIYDQLKQAGLQPVAFEGITEVTAAQIKSSP
                     EGVRMLAKKALEYKADYLMHFDVEADNRPFSQGGYVCDANFIYTLMRPYDNTILGESL
                     FTGREGGNSSMVAFTNTLESVKDQLVLQAVGQLFRSIFEDSDVITDTKQLKNAFTLTV
                     FFKDNPGQVQAIIDGLQALGATVQLGVGAGAGDRLQIETTMDQLALYNYLNDQTFSAN
                     GVKFKSPVGDYTENTISVEIVKAGAPAKKPVVKNTPPPRKEQQADLGSTGKKGKPIIL
                     KLKGNVRPPSFA"
BASE COUNT          291 a          293 c          305 g          330 t
ORIGIN      
        1 ataaaatcgc tgcagagagg caggattata gcattcgaac cacgcaattc gtaaaccggg
       61 cttagtggaa tgtacaccag tcagtagtca gtagtcagta gtcagtagtt cgttaagttt
      121 atctggttga attacttgac tattttctaa tacgagacct tcattgcaaa ttggcataag
      181 aaatagtggg gctgaagtct ccgaactgaa gagaacgggc tcagagcctt gggttgaaga
      241 agtcgggctg agggctaaag aactggtttt atttcatccc tcatccttca tccctcatcc
      301 cttcgagtgc cccaggccca atgtcttcag cctgatgatg tttcaccccc actaagcaaa
      361 tgacggtggg cgcacatttc ctttgagctt caaaatgatg ggcttccctt ttttgccagt
      421 tgaaccaagg tcagcctgct gctctttccg aggtggtggg gtgtttttca caaccggttt
      481 tttcgctggt gccccggctt tgacaatttc aaccgaaatg gtgttttcgg tatagtcccc
      541 cactggcgac ttgaatttta caccatttgc gctaaacgtc tggtcattca ggtagttata
      601 cagcgccagt tgatccattg tggtttcaat ttgcagccgg tcaccggcgc cggcgccaac
      661 gccaagctgg acagtggcac caagggcctg caagccatca ataattgcct ggacctgacc
      721 ggggttatct ttaaagaaaa ccgtcagggt aaaggcattt ttgagctgtt tggtgtcggt
      781 aatcacatcg ctgtcttcaa aaattgaccg gaacagttga ccgacggctt gcaaaaccag
      841 ttgatctttg acggattcga gcgtgttggt aaacgccacc atagacgaat tcccgccttc
      901 acgcccggta aataatgatt cacctaaaat cgtgttgtca tagggtcgca tcaaggtgta
      961 gatgaaattg gcatcgcaga catacccgcc ctgactaaac gggcggttat cggcttcgac
     1021 gtcaaagtgc atcaggtaat cagctttgta ctcaagcgct tttttggcca gcattcgcac
     1081 gccttccgga gaggatttga tttgggcggc agtcacttcg gtaatgcctt caaaggccac
     1141 tggctggagg ccggcctgct tgagctgatc ataaatcata gcccgcagct tttttgcggt
     1201 gagcggatcg gtaaaactt
//
LOCUS       DAOQDO010002304         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_147142_length_1217_cov_3.602410, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002304 DAOQDO010000000
VERSION     DAOQDO010002304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147142_length_1217_cov_3.602410"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..>1217)
                     /locus_tag="PKZ53_26815"
     CDS             complement(48..>1217)
                     /locus_tag="PKZ53_26815"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44125.1"
                     /translation="LVEFLVHHPYDLKLKQNGSPHYIAYILTPTGDPKWVDLGDAAAI
                     DELVMKFRSALSDRSQKGIAGFVPLSQKLYQIIFNPVVKLAGQPQRCFLSPDGMLNLL
                     PFAALMDHRQKYVVEQFKLTYLTSGRDLLHLKIKAPSQTPPLLVVDPDYAEGPGPQLL
                     GVTYPPLVRLPGTAREGKQVQQYFENTQLKSGAEATEQALKAADRPLLLHIATHGSFL
                     SDVAPPPVEPESQRNLTPVGSGLDMDQVRNTHSLLRSYLFFAGANTRSSSDNDGTLTA
                     LEAANLNLWGTKLVVLSACNTGVGEVKTGEGVYGLRRALVLAGSESQMMSLWPVSDQG
                     TQVLMTEFYRRLKASEGRSDALQNTQLWMLNHPRWKHPFYWASFIQSGEWKPISE"
BASE COUNT          281 a          325 c          317 g          294 t
ORIGIN      
        1 attcttcatt cttcattctc aattcttcat tctcaattct tcattcttca ttctgaaatt
       61 ggtttccact cgccggattg gataaaggac gcccagtaaa atggatgttt ccagcgggga
      121 tggttgagca tccagagttg cgtgttttgg agcgcatcgc tgcggccctc actggctttc
      181 agacggcggt aaaattcggt catcaggacc tgtgttccct gatcggaaac tggccacaga
      241 ctcatcattt gcgattcgct cccggccagg acgagtgccc gccggaggcc atacacgcct
      301 tcaccggttt tgacttcgcc cacgccagtg ttgcaggccg agagcacaac cagtttggtg
      361 ccccacaaat tgaggttggc agcttccagg gcagtcaagg tgccgtcatt gtcgctgcta
      421 ctgcgggtgt tggccccggc aaaaaacaga tatgaccgca acaacgaatg tgtgttccga
      481 acctggtcca tgtccaaacc tgacccaacc ggcgtcaaat tgcgctggga ttcaggttcg
      541 actggcggtg gggccacatc gctcaggaat gaaccgtggg tggcaatatg caggagcaac
      601 ggacgatcag ccgctttgag cgcctgttca gtcgcctcag cccctgactt gagttgcgtg
      661 ttttcaaaat actgctggac ctgtttacct tcgcgggcag ttcctgggag acgaaccagc
      721 ggcggatagg tgactccgag caattgcggc cctggaccct cagcataatc cggatcaacc
      781 accagcaatg gcggggtttg agatggcgct ttgatcttga gatgcaacaa atcacggcca
      841 ctggtcagat aggtcaattt aaactgctca accacatact tttggcgatg gtccatcagt
      901 gcggcaaagg gcaacagatt taacatccca tctggtgaca gaaagcaccg ttgcggctga
      961 ccagccagtt tcacaactgg attgaaaatt atctggtaga gcttctgcga aagtggaaca
     1021 aacccggcaa ttcccttctg gctgcgatca ctgagtgcgc ttcgaaactt catcaccagt
     1081 tcgtcaatgg cggcagcgtc cccaaggtcc acccatttcg gatctcctgt cggtgtcaga
     1141 atataagcaa tatagtgtgg tgacccattc tgtttgagct ttagatcata tggatgatga
     1201 acgagaaact cgaccag
//
LOCUS       DAOQDO010002305         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_147161_length_1217_cov_3.068847, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002305 DAOQDO010000000
VERSION     DAOQDO010002305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147161_length_1217_cov_3.068847"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PKZ53_26820"
     CDS             complement(<1..>1217)
                     /locus_tag="PKZ53_26820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44126.1"
                     /translation="WTALNVTNSGLPSNIVNQFIEVAGPDGEPELWVGTSAGLAHYAH
                     EKWTVYNEENSGIPTNFVYSVRQTRTPDGTTTIWVGTSGAGLGAFSNGKWTRYTTSNS
                     QLPNNAVLSLAATTEPDGRMFLWAGTQGGGATRIDLLNPSAEWLILSDSSDPVRLPSN
                     VIHKILQDQPHRLFLFTNKGIARLTWQVSTAENPTGYSLYTYTTEDGIPASLSTAVMI
                     DRSGRIWAGTSSGAAYLDLNREIQDRSPKPLTIERFTANGLDLPFTDSELTLHHDQNN
                     LAFEFALLCYLHEDDVRFQVQLEGYDSAPTGWIHDRKVTYTNLGAGRYTFKVTGRDVF
                     GNITPPTTLKILIRPAPWLTWWAFLGYFLLIGGTGIGLYQWRVSILRKRQEERLRDLL
                     QLLESTRVINSNL"
BASE COUNT          287 a          313 c          337 g          280 t
ORIGIN      
        1 ccagatttga gttaatcacc cgtgtacttt caagaagctg gagcagatct ctgagtcgtt
       61 cttcctgacg tttgcgcagg atactgaccc gccactgata taacccaatc ccggtacctc
      121 cgatcagaag aaaatagccc agaaacgccc accaggtcag ccagggggct ggtcgaatga
      181 ggattttgag cgtcgtggga ggcgtgatgt ttccaaacac gtcccgaccc gtgaccttaa
      241 atgtatacct gccggcgccc aggttggtgt aggtcacttt tcggtcatgg atccagcccg
      301 ttggggcaga gtcatagcct tcgagttgaa cctgaaaccg cacatcgtct tcgtgcaaat
      361 agcacaaaag ggcaaattca aaggccagat tattttgatc gtggtggagt gttagctcag
      421 agtcagtgaa tggaagatca agaccgttgg cggtaaagcg ctcaatcgtc aatggcttcg
      481 gagatcggtc ctggatttcg cggttgagat ccagataggc ggcgccggaa ctggtcccag
      541 cccagattct gcccgaacga tcaatcataa ccgccgtcga aagactggcc gggatgccat
      601 cttcggtggt gtaggtatag agcgaatatc cagtcggatt ttcagccgta ctcacctgcc
      661 acgtcaaccg ggcaatccct ttattggtaa acaagaagag tcggtggggc tggtcttgca
      721 ggattttatg gatgacattg ctcggaagcc gaaccgggtc tgaactatca gataaaatca
      781 accactccgc cgaagggttg agcaggtcaa ttcgggtggc gcctccgccc tgtgtgcctg
      841 cccataaaaa catccggcca tccggttcag tggttgccgc cagagacaga accgcattat
      901 tggggagttg tgaattggac gtggtatagc gagtccattt tccatttgaa aaggcgccta
      961 agccagcccc actggttcca acccaaatgg tggttgtgcc atcgggagtt cgagtttgac
     1021 gaaccgaata cacaaaattg gtgggaatcc ccgagttctc ttcattatag acggtccatt
     1081 tttcgtgggc gtaatgggcg agcccggcgg atgtccccac ccagagttcc ggctcgccgt
     1141 cgggcccggc aacctcaata aactgattga caatgttgct gggcagaccc gagttggtga
     1201 cgtttaatgc cgtccac
//
LOCUS       DAOQDO010002306         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_147431_length_1216_cov_2.964686, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002306 DAOQDO010000000
VERSION     DAOQDO010002306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147431_length_1216_cov_2.964686"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1216)
                     /locus_tag="PKZ53_26825"
     CDS             complement(<1..>1216)
                     /locus_tag="PKZ53_26825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44127.1"
                     /translation="SNDPEFNPRVVDQITLPSGHTWNFKYNAYGEVTEVELPSHGFIR
                     YTISETSPSTERLFRWVSKREVWTSNTQMEGKTVYVRDDYNQSPGSIVTERRLDAGNT
                     LLSKVEHIFSDSPLNQYAVGFGVPGGTGYKPWRQGKELETVTYDSDGTTKLQTISYAW
                     EQRDSVSWLSSPPPGRNNLSQPEQDPRMSSVETSLDDTGGAPQISRVTYQYDDDVSNG
                     YNGNGFNNVTREQMFGYNGELLREIRRSYIGQLNGTNYQTDPNAHLRSLIHKESIWNA
                     NGEQETFSQYGYDEFDPQPITLTDSSRNQNRGINYKTRGNVTSVTVGGPNTVSSVSSM
                     RYDVAGNVIYTEGPPTTGGRKLSIETTYDGALRFAYPRQTSTRVTEVDQTQQELTTST
                     DYDFSTGVVTSTR"
BASE COUNT          266 a          302 c          289 g          359 t
ORIGIN      
        1 cctggtcgaa gtcaccacgc cggttgaaaa atcatagtca gtgctggttg ttaactcttg
       61 ttgagtctgg tcaacttctg tcactctggt gcttgtttgc cgtgggtaag caaatcgcaa
      121 cgctccatca taggtggttt ctatgcttaa tttccgccct ccggttgttg gcggtccttc
      181 agtgtaaata acattcccgg ccacgtcata gcgcattgac gacactgacg acactgtatt
      241 tggcccacct acggtgaccg atgtaacatt acctcgtgtt ttatagttta taccccgatt
      301 ctggttgcgt gaactatcgg tcagagtaat tggttgtgga tcaaactcat cataaccata
      361 ctggctgaag gtttcctgtt ccccgtttgc attccatatg gactctttgt ggatcaatga
      421 ccgtaagtga gcattggggt cggtctggta attggttccg ttgagctgac caatgtagct
      481 ccggcggatt tcccgaagca gttctccgtt atacccaaac atttgctctc tggtgacatt
      541 gttaaatccg ttgccgttat acccattgct cacatcatca tcatattgat aggttaccct
      601 tgaaatttgt ggcgcacctc ccgtgtcgtc caaactggtt tccaccgaac tcatgcgtgg
      661 atcctgttct ggctggctca aattattacg acctggtggt ggtgaactga gccaggaaac
      721 cgaatcgcgc tgttcccagg catagctgat cgtttggagt ttggttgtgc catcactgtc
      781 ataggtcact gtttccagtt ctttcccctg cctccatggc ttgtatccgg ttccgcctgg
      841 cacaccaaac ccaaccgcat actgattcaa aggcgaatct gaaaaaatat gctcgacttt
      901 tgacaaaagt gtattgcctg catccaaccg acgctcagtc acaattgatc caggagactg
      961 gttgtagtca tcccgcacat agacggtctt cccttccatc tgagtgttgc tggtccagac
     1021 ttcacgtttg gaaacccacc ggaagagacg ctcggtactt ggtgaggttt ccgagatggt
     1081 gtagcgaata aagccatggg atggaagttc tacctcggtt acttcaccat aagcattgta
     1141 tttgaagttc catgtatgcc ctgacggaag ggtaatctga tctacgacgc gggggttaaa
     1201 ttctggatcg ttggat
//
LOCUS       DAOQDO010002307         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_147882_length_1214_cov_3.410699, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002307 DAOQDO010000000
VERSION     DAOQDO010002307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147882_length_1214_cov_3.410699"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKZ53_26830"
     CDS             complement(<1..353)
                     /locus_tag="PKZ53_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44128.1"
                     /translation="MKQQSNVSLRRIAFQFGAILLFLLVVTPNLEAQQKRPSKPRSRK
                     STKAPNPQPALAFTGEPVQERNVRAHLEFLASDALQGRASATQFERIAGQYIAAQFRQ
                     FGIEPAGEKDKSGNVT"
     gene            complement(377..>1214)
                     /locus_tag="PKZ53_26835"
     CDS             complement(377..>1214)
                     /locus_tag="PKZ53_26835"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNJ44129.1"
                     /translation="LRSRRAGSILQEAERLAAEGVKELILIGQDTTSYGEDLGVKDGL
                     ANLLKELAKIEGLQWIRFLYSYPTRLSNAVLDVMAAEPRLCKYVDIPLQHASTRMLHA
                     MRRPGSREFLEKMVRRIRTHVPDIALRTTFIVGYPGETEADFEELVSFCKEQEFDRVG
                     VFTYSDEDSTPAFNLADKVPARTAESRRKKLMKVQEQISKRRNKRFLGKTVDVVFEGL
                     SEESDLIWQGRMATQAPDIDGHVLITDTPEGFNPEIGQLVRVEIMETHAHDLVGHIVA
                     AG"
BASE COUNT          296 a          320 c          305 g          293 t
ORIGIN      
        1 gtcacattcc cggacttatc tttttcaccg gcgggttcaa tcccgaactg acgaaactga
       61 gcggcaatgt actgaccggc gatgcgttca aactgcgtgg cactggctct tccctgaagc
      121 gcatcacttg ccaaaaactc aagatgagcc cggacgttac gttcctgaac cggttctccg
      181 gtaaaagcca gtgcaggttg ggggttgggg gctttggtgg acttgcgcga gcgcggtttt
      241 gaaggccgtt tttgttgagc ttcaagattg ggcgtgacaa ccaaaagaaa aaggagaatt
      301 gcaccaaact ggaaagcaat tctccgcaaa gacacattgg attgttgttt cataaacgtg
      361 ggggtggaag cacctttcaa ccggcagcaa caatatgacc aaccagatcg tgggcgtggg
      421 tttccataat ctcaacccgg acaagctggc caatttcagg gttaaaccct tcaggcgtat
      481 cagtaatgag cacatggcca tcaatatccg gtgcctgggt tgccatgcgt ccctgccaga
      541 ttaaatcgct ttcctcagat aacccttcaa acacgacatc aaccgttttt cctaaaaagc
      601 gtttgttgcg gcgtttggaa atctgctcct gaactttcat caattttttg cgacgggatt
      661 cagcagtgcg ggctggaact ttgtcagcca gattgaaggc cggcgtggag tcttcgtccg
      721 agtaagtaaa cacacccacc cgatcaaatt cctgttcttt gcaaaaggaa accagttctt
      781 caaaatcggc ttcggtttca cccggatagc ccacaataaa cgtggttcgc agggcaatgt
      841 ctggaacgtg agtccgaatc cgacgcacca tcttttcaag aaattcacgc gacccaggcc
      901 gccgcatcgc gtgaagcatt cgcgtgctgg catgctggag cggaatatca acgtacttgc
      961 agagtctggg ttctgctgcc atgacatcca gcacggcatt gctcaatcga gtggggtaac
     1021 tatagagaaa ccgaatccac tgcaagcctt caattttggc taactctttg agcagattgg
     1081 ccaagccatc tttgacgccg aggtcttcgc catagctcgt tgtatcttgc ccaatgagaa
     1141 tcaactcttt gacgccttcg gcagccagcc gctcggcttc ttgtaaaatc gaaccggccc
     1201 gccggctacg cagg
//
LOCUS       DAOQDO010002308         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_148188_length_1213_cov_2.373057, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002308 DAOQDO010000000
VERSION     DAOQDO010002308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148188_length_1213_cov_2.373057"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKZ53_26840"
     CDS             complement(<1..240)
                     /locus_tag="PKZ53_26840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44130.1"
                     /translation="MSSYCVGSRRLTAWLISMALLIPFGLVEYCAVPIPAMAQSSGQK
                     DERPTKPALKKSPGAQSDDDGILIKSDLVLLDVAVF"
     gene            776..>1213
                     /locus_tag="PKZ53_26845"
     CDS             776..>1213
                     /locus_tag="PKZ53_26845"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HNJ44131.1"
                     /translation="MDKTQWEAFLLELEKRINHESFNTWFKPLTFHSSTDAEYNIGVP
                     NELFRNWIEEHYSEILEESLVAIGISHLVPALVITDQATEATANNQSAAVPDIYHPPT
                     NGSRSLYPAEPANSNANTRYLDLEPTELPLNPKYTFETFVVGSC"
BASE COUNT          352 a          288 c          264 g          309 t
ORIGIN      
        1 aaaaaccgcc acatccagca ggaccaggtc agatttgatc agaatcccgt catcatctga
       61 ctgggcgcca gggctttttt tcagagctgg tttggtgggg cgctcatctt tttggccaga
      121 ggattgggcc atagcaggga ttggaacggc acaatattca accaggccaa atggaatcaa
      181 aagagccatt gaaatcagcc aggcagtcag acgacgggaa ccaacgcaat aggacgacat
      241 agcgcgcaca tacctccaga cagaaaaacc agttcagata gaaaaattgt tgattggtag
      301 acaagaactc caaaccatta aggaaagcac gaaccaatgt gggaagccag tttctgtccc
      361 cagcctcatg tcggattctt acagagcttt atggcgttgt ctatttggca attacgaaaa
      421 aggtcttagc tttaaggtat ttcgagaaat tcgaagtttg acgggtacac tcacccgctg
      481 atctgattta aacgcatcag gatttttcca aagccacaca agcgcttaga aaaagcctgt
      541 ggatttcttc ttgtgctccc cccttcgcta tgctagtctg gcaattgctc aaaagaactt
      601 ttttccagcc taagtcaatg taaggtaaca acttacagtt ttcctttaag tcttcaacac
      661 cctcattttg ccctgtggaa aacctgtgga aaacctgtgg aaaaagttcc tttttcactt
      721 ttgaaacaag cgatttaacc ctcaaatatt tcagacttgg ttgaagagtt gcagtatgga
      781 taaaactcaa tgggaagctt ttttgttaga actcgaaaag cggattaacc atgaaagctt
      841 caatacctgg tttaaacctt tgacgtttca ttcgagcacc gatgcagagt acaacatcgg
      901 cgttccaaat gagttatttc gaaactggat tgaagaacac tatagcgaga ttttagaaga
      961 gtcactggtt gcgattggga tttctcattt ggtgccagcc ctggtcatca ccgatcaggc
     1021 aaccgaagcc acagccaaca atcagagtgc agcagttcca gatatttacc acccaccaac
     1081 caatgggtcg cgttcactct atccggctga accggccaac tcaaacgcca atacccggta
     1141 tctggacctg gagcccactg agttaccgct caatccgaaa tacacgtttg aaacctttgt
     1201 ggtcggttcg tgc
//
LOCUS       DAOQDO010002309         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_148710_length_1211_cov_2.332180, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002309 DAOQDO010000000
VERSION     DAOQDO010002309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148710_length_1211_cov_2.332180"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKZ53_26850"
     CDS             complement(<1..345)
                     /locus_tag="PKZ53_26850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44132.1"
                     /translation="MRDQFQLKQSVVSAGPRRAWIFVLMVMFFNCLALVGQQESGSVR
                     DIKRTYSNFDQLRPKATSKNQFSAKKSESFSKSVPKSLPTNLSASDQYLVGITLWKLR
                     PQGMGDPKPEVPI"
     gene            complement(538..1098)
                     /locus_tag="PKZ53_26855"
     CDS             complement(538..1098)
                     /locus_tag="PKZ53_26855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44133.1"
                     /translation="MLKHFILALNILFVLSFNLMMTTQATQAQEVTNQHKPYRIYKGV
                     QPITATAALQTVVILDHELKKVDVVVQVAEFDQIKRRAQFTLNNFEVTDTTVRVAIPF
                     RQTDLQIGEPGQSVMLHLTAANVQDATTWNLTIETKGVQGPTQIGVDQVIDPYQATCE
                     RACYLYAPPGERSACLLGCVLNSIFK"
BASE COUNT          317 a          268 c          299 g          327 t
ORIGIN      
        1 aatcggcact tctggtttgg ggtctcccat tccttgaggc cgcaatttcc aaagggtaat
       61 cccgaccagg tattgatctg atgctgaaag attggttggg agactttttg gaacgctctt
      121 cgaaaaactt tctgactttt tagctgagaa ttgatttttt gaagtggctt tggggcgtaa
      181 ctggtcaaaa tttgagtacg ttcgtttgat atcccgaact gaacctgatt cttgttgtcc
      241 caccagtgcg agacaattaa aaaacataac catcaggaca aaaatccagg cacgccttgg
      301 cccggcagat acaaccgatt gtttcagttg aaattgatcc cgcatagagg ttttccatcg
      361 ttgacggagc tcgttgtgag tgggtaccgt gggtactatg aatgaatgaa gaaaaagaag
      421 ccaatttgga tcctgggttt tccatatttt aatgtcaaac cagttcgaac ttaaaaattt
      481 aggaaaagga aacagagtgg aacggagctt tactccgctc cacctggttg gacaaatcta
      541 tttgaaaatc gaatttaaca cacagcccag caagcacgca ctgcgttccc ctggcggagc
      601 atacaaatag caggcacgtt cacaggtggc ctggtacggg tcaatcacct gatcaactcc
      661 gatttgggtg gggccctgaa cgcctttggt ttcaatcgtt agattccagg tggtggcgtc
      721 ctgaacattg gcggcggtca ggtgaagcat caccgattgg ccaggttcac caatctgcag
      781 gtcggtctgg cgaaacggaa tcgccacccg gaccgtggtg tcggtcacct caaaattgtt
      841 taaagtaaat tgtgcccggc gtttgatctg gtcaaattca gccacctgca ccacaacatc
      901 aacttttttc aattcgtgat ccagaatcac aaccgtttgg agcgccgccg tggcggtgat
      961 gggttgaacc cctttgtaga tccgataggg tttgtgctgg ttggtaactt cctgagcctg
     1021 ggtggcctgg gtggtcatca tcagattgaa gctaaggaca aacagaatat tcaaggctaa
     1081 aataaaatgt ttcaacatac ggatgctcct ttaattaact cataatagtg acttatcaag
     1141 tgtgaatacc gtgaggattt catttcctcg ttcactggat aagtcggaaa aggagcgcct
     1201 gagccttcat c
//
LOCUS       DAOQDO010002310         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149263_length_1209_cov_1.980936, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002310 DAOQDO010000000
VERSION     DAOQDO010002310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149263_length_1209_cov_1.980936"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1025
                     /locus_tag="PKZ53_26860"
     CDS             297..1025
                     /locus_tag="PKZ53_26860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HNJ44134.1"
                     /translation="MANQSLQVATPTVNLVNGIPQTTSLDIADHFGKLHKNVIQAIDR
                     LECPEDFRRLNFQPSSYLNEQKRDQPMYNITRDGFVILAMGFTGKQAMEWKIKYIQAF
                     NAMETRMKGQPQLPPSSPARSVLPIRIESNRPLFHYPLESQFSDQEKLGFTRAGAMTV
                     ILQTTTHLLKKEMNSPLRKLLYQIVQENMGDVRGCIEELDCLYFQLRSIHTHVESLRN
                     LSNEMQNKVERLLNEGRTIVVKKG"
BASE COUNT          329 a          328 c          267 g          285 t
ORIGIN      
        1 ctaaatccga aagttggtgt tcagaaaagg ctcgtatccc tcaaaaaagc aaattccctt
       61 caaacttgac accttccccg tcctcggcta ccctgcgttt ggtgcttcaa aacacctcta
      121 gcggtataca cgcccgtcag ccgtgatttt tttgtgccct ttttccgatt tgcgtcgggg
      181 tgggcacgtc tataaaacaa ccgcaaggtg aaggacgtgt cgccgttcct agagcggttt
      241 tgaacacccc ggctcttacc aagcctggaa attcaaaaaa atctaggagg ccatctatgg
      301 ccaatcaatc tctgcaagtt gcaaccccca ccgttaacct cgtcaacgga atcccccaaa
      361 ccacatccct cgacatcgct gaccacttcg gaaaacttca caaaaacgtc attcaggcca
      421 ttgatcggct tgagtgccca gaggattttc gacggctgaa ttttcagccg tcctcgtatc
      481 tcaatgagca gaaacgagat caaccgatgt acaacatcac tcgtgatggc tttgtcatcc
      541 tggcaatggg gttcacaggc aagcaggcaa tggagtggaa gatcaaatac attcaggcgt
      601 tcaatgcgat ggaaactcgc atgaaaggcc agccgcaact ccccccatca tcacctgccc
      661 gctcagtttt gccaatccgc attgaatcca accgcccgct tttccactat ccactggaat
      721 cacaattcag cgatcaggaa aaactgggat ttacccgagc aggagcaatg accgtcatcc
      781 ttcagaccac aacacaccta ctcaagaagg aaatgaacag cccgctccga aagctcttgt
      841 atcagatcgt ccaggaaaat atgggagacg tcaggggctg cattgaagaa ttggattgcc
      901 tgtatttcca gcttcggtca atccataccc atgtggaatc cttgcggaac ctgtccaacg
      961 agatgcaaaa caaagttgaa cgactactga acgagggccg aacgattgtg gtcaagaaag
     1021 gttaactagg tgcgtaaatt agtcacctga tttttcgact tattcccgag taatagagcc
     1081 caccaggggg gacttggtgg gctttttgct ttttggcgac caaaaacgac ttctcaggta
     1141 ctggtagctt cacctcattt caaactgagg tgagttatgc cggatccaat tgagctgata
     1201 taccgactg
//
LOCUS       DAOQDO010002311         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149303_length_1209_cov_1.755633, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002311 DAOQDO010000000
VERSION     DAOQDO010002311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149303_length_1209_cov_1.755633"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKZ53_26865"
     CDS             <1..327
                     /locus_tag="PKZ53_26865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44135.1"
                     /translation="PARRAMESSRWAACFEAHPPENRPHMVRARMGAGSNLQGIWSLH
                     QNLADEHFQRPSGRERKTSVRWYAQSGKPPATLRRPSGRKPTFPQQSLRRLKNQSCCS
                     LETMPG"
     gene            404..772
                     /locus_tag="PKZ53_26870"
     CDS             404..772
                     /locus_tag="PKZ53_26870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44136.1"
                     /translation="MPNLIVVKHESFPKRCEVCHQADFFNPKTNHCARCKQISRKFQN
                     LSRTSNKKSTLSIFQVVKRVLAIGGGSFLGFVLHVEMISPTGGPCNPGSALLIIPIFG
                     FWCLIFILGLVVFRPRNSSK"
     gene            801..1157
                     /locus_tag="PKZ53_26875"
     CDS             801..1157
                     /locus_tag="PKZ53_26875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44137.1"
                     /translation="MPDEFVIKHETLPVRCEVCHQADFFEPEANHCARCGHISREFST
                     ERQALSSTKRGLIWLLSIFGVALFGIFYYVDHLPRSKNPCGGDQLIILPLLMIWGFLL
                     FFALLVYFSSSPNNSK"
BASE COUNT          298 a          299 c          289 g          323 t
ORIGIN      
        1 cccgcccgga gggcgatgga aagtagccgg tgggcagcct gctttgaggc gcacccaccg
       61 gaaaacaggc cacacatggt tcgcgcccgg atgggcgctg gatcaaacct ccagggaatt
      121 tggagccttc accaaaacct tgccgacgaa cacttccagc gcccatccgg gcgcgagcgg
      181 aagacatcag tccggtggta tgcccaaagc ggcaaaccac cggctactct ccggcgccca
      241 tccgggcgaa aaccgacctt tccgcaacag agtttaagga ggttgaaaaa ccagagttgt
      301 tgcagtcttg agactatgcc aggctaaagt tggaactctg tacttttttg caattgttcc
      361 tggtaatcaa tccccataaa tcagtatcgt attttctaag gttatgccca atctcattgt
      421 cgtcaagcac gaatcattcc caaaacggtg cgaagtctgc catcaggctg atttcttcaa
      481 tccgaaaacc aatcattgtg cccgatgtaa gcaaatttcc aggaagtttc agaacctgtc
      541 ccgcacctcg aacaaaaaaa gcacgctttc aatttttcaa gtggtaaagc gcgtgttggc
      601 catcggcggc ggatcattcc ttgggtttgt attgcatgtg gaaatgattt ctcctactgg
      661 cggcccctgt aatccaggga gtgcgcttct gatcattccc atctttggct tctggtgtct
      721 gatatttatt ctggggctgg ttgtttttcg ccctcgaaat agttcgaaat aaccgtccat
      781 ttccagattg agaaaaacca atgcctgacg aatttgtaat caaacacgaa accctcccgg
      841 tcagatgcga agtctgccat caggctgatt tctttgagcc ggaagccaat cactgcgcac
      901 gatgtggcca tatctcaagg gagttttcga ccgaacgaca ggctttaagt tcaaccaaaa
      961 gagggttgat atggctgctt tcgatcttcg gcgttgcgct ctttgggatt ttctattacg
     1021 tagaccatct ccctcggtcg aagaatccgt gcggaggtga tcaattgatt atcctgccac
     1081 tgttaatgat ctggggattt ttgttgtttt ttgcgctctt ggtgtatttt tcgagtagtc
     1141 caaataattc aaagtgaatc aggaaaagat tcagcctatg ctgttgagac aaaaagtttt
     1201 caatcagtt
//
LOCUS       DAOQDO010002312         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149421_length_1208_cov_3.205551, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002312 DAOQDO010000000
VERSION     DAOQDO010002312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149421_length_1208_cov_3.205551"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PKZ53_26880"
     CDS             complement(<1..1159)
                     /locus_tag="PKZ53_26880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44138.1"
                     /translation="MFQSNSRWGENLRLASFGLLTMGLAGCYWLGGFGLTSIGGEEAV
                     FKFLSVFGMAFALYLLTLVLLHRVSNHVHSGWLVGWILGAAIVFRIFLLPAGISDGVT
                     WEKLRADVTGSEVVYDRFLLYDHDVWRYLWDGHVQSVGMNPYGYAPAELEPGAGLVFE
                     ANPHLEAQLFPTDTWSDIWDNLNYKEIRTVYPPLLQVAFRLSHALAPGSVLVWKLILV
                     VCDVGLCLVLLSLLKALNQPIWLLAGYAWNPLVIKEITGSAHADVIPALMIALAVLGL
                     VKQKPLWAGGWLAGGILAKLAPAVLIVLVWKRLGWLGLVVCGAVTAIGFLPFFLTGDS
                     LFGGLGTYSQEWIFNPGFFELVRWLAMWLTPSINPSTAGKVIGGVCYLGLLV"
BASE COUNT          335 a          382 c          274 g          217 t
ORIGIN      
        1 acaccagcaa tcccaaatag caaacaccgc cgatcacttt tcccgccgtt gatggattga
       61 ttgacggcgt cagccacatc gccaaccacc gcaccagttc aaaaaagccg gggttaaaaa
      121 tccattcctg tgaataagtt ccaagccctc caaacaggct gtccccagtc agaaaaaatg
      181 gaagaaaacc gatggctgtc accgcaccac acacgaccag ccccaaccac cccaggcgtt
      241 tccaaaccag aacaatcagg accgctggtg ccagtttggc caaaataccg cccgccagcc
      301 agcccccggc ccaaagtggt ttttgtttta caagccccag taccgccaga gcaatcatca
      361 aggctggaat gacatcagcg tgggccgaac cagtgatctc cttgatgacc agcgggttcc
      421 aggcatatcc ggccagcagc cagatgggct gattcagagc tttcaacaaa ctgagcagca
      481 ccaggcacag gccaacatca cacaccacga ggatcagttt ccacaccagc acactccctg
      541 gcgccagcgc gtgtgagagc cgaaaagcaa cctgcaacag tggcgggtag accgtccgaa
      601 tttctttata gttgaggttg tcccagatat cactccaggt atcggttgga aagagctgtg
      661 cttcaaggtg agggttggct tcaaacacaa ggccagcccc tggttcaagt tccgccgggg
      721 catacccata cgggttcatt ccaaccgatt gcacgtgacc gtcccagaga tagcgccaca
      781 catcgtggtc atagagcaaa aaacggtcat acaccacttc gcttccggtc acgtcggccc
      841 tcagtttttc ccaggtcaca ccgtcgctga tgcctgccgg aagcaggaaa attcgaaaga
      901 caatggcggc tcccaaaatc catccgacca gccagccaga gtgaacgtga tttgaaacgc
      961 gatgcaacag cactaaagtc aggagataca gcgcaaaggc catcccaaac acgctgagaa
     1021 acttgaaaac cgcctcttcc ccaccgatac tggtcagacc aaatccacca agccagtaac
     1081 aaccagcaag gcccatcgtg agcaagccaa agctcgcaag ccgcaggttc tcaccccatc
     1141 tggaattgct ctgaaacatc tgttgaggct ccgtctccat gaaaatcctg tctctgctcc
     1201 tcagtctg
//
LOCUS       DAOQDO010002313         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149463_length_1208_cov_2.568951, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002313 DAOQDO010000000
VERSION     DAOQDO010002313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149463_length_1208_cov_2.568951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKZ53_26885"
     CDS             complement(<1..370)
                     /locus_tag="PKZ53_26885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44139.1"
                     /translation="MSHSTPPQFQIKNEGLAARCEVCHQGDQFDPESGYCRRCGYVTG
                     EKSSSRAIAQYRNIGVELELMKPQIYVLGGIIFCVAFAAGAYWQSQWIFIVGTMVLLG
                     VISHFQQKKKELEARQKNLES"
     gene            833..>1208
                     /locus_tag="PKZ53_26890"
     CDS             833..>1208
                     /locus_tag="PKZ53_26890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44140.1"
                     /translation="MNFSDYTEDRICQEFGFPSFASDPFLVNSKLSLRLLLQPSFDAE
                     VCISLVICGPTATLQVAAFAEAFQYQPSPAWHPAFDLELCDIPLESTQTLIVSTEETL
                     RNLPKRGLDIIDGMGFSLVILKK"
BASE COUNT          325 a          308 c          248 g          327 t
ORIGIN      
        1 tagattcgag atttttttga cgggcttcca gctctttttt cttttgctga aaatggctta
       61 ttactccaag cagcaccatt gttccaacaa taaagatcca ttgactttgc cagtacgccc
      121 cagccgcaaa tgccacacaa aagattatcc caccgagaac atagatctga ggcttcatca
      181 actcaagttc tacgcctatg tttctatatt gtgcgatggc ccggcttgaa ctcttctccc
      241 cagtcacgta gccacaacga cggcagtacc cggattcagg atcaaactga tctccttgat
      301 ggcaaacttc gcaccgggca gccagccctt catttttgat ttgaaattga ggcggagttg
      361 agtgcgacat aggaaccaca caattatttt cgttcagtca tttctgagct gagtactgca
      421 aaaccggcaa aattttcctt ttcgactgct gcccaacgga gactttcgat ggcactgcgg
      481 acaataatct gagcttaacc actgtgttcc aaatatcaaa agcaacgcca gagtcatcca
      541 gccaaaagca aacaacacaa agccaaaata aaaaaagaac acaaaacaca gagtcagtcc
      601 ggataaagtt atgatttgat tgatttttcc ctgtgatact actcgccggt caatcttttc
      661 aaatgcctgc agaatttctg gtgtggtttg cttccctttt cctgggttac acttccgaca
      721 aataccggtt tgaggatccc aggtatctgg ctgatcgcag attggacata aaacagttgg
      781 cgaaacaagg gtcatggagg aaaagccctc aaagcaaata actggaggag cgatgaattt
      841 ttcagactac accgaagatc gaatttgtca ggagtttgga tttcccagtt ttgcctcgga
      901 cccatttctg gtcaatagca agctctcctt gagactcttg cttcaaccgt ctttcgatgc
      961 cgaagtctgt atttcgcttg tgatttgtgg tccaaccgca acccttcaag tcgcagcgtt
     1021 tgctgaagcc ttccagtatc aaccatctcc ggcctggcac ccggcgtttg acctcgaatt
     1081 gtgtgacatt ccactcgaat caacccaaac cctgattgtc tcgactgaag aaaccctgcg
     1141 caaccttcct aaacgtgggc ttgatatcat tgatgggatg ggcttttccc tggtaatcct
     1201 caaaaagg
//
LOCUS       DAOQDO010002314         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149479_length_1208_cov_2.386817, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002314 DAOQDO010000000
VERSION     DAOQDO010002314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149479_length_1208_cov_2.386817"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1208)
                     /locus_tag="PKZ53_26895"
     CDS             complement(109..>1208)
                     /locus_tag="PKZ53_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ44141.1"
                     /translation="ELVAIFDADFRPRPDCIRKMVHYFTEEKVGVVQFRWSHINADYN
                     VLTRIQSVLLDGHFVIEHTSRNRSGGFFNFNGTAGMWRRQAIEWSGGWQHDTLTEDTD
                     LSYRAQMLGWKFVYVMDEDVPAELPVEINAFKAQQRRWAKGLTQVAFKMFKRTLNSDL
                     PWHVRIEMFFHLTSNLSYPLMIVFNLLHFPILIVRYNQGLFQMMLLDIPFLLLSTFSV
                     TSFYYISLKELHGKKGAKLWMIYLVMATGVGLSMSNAKAVMEAVFGIKSSFVRTPKYA
                     IETAKDSWQNKKYRRNRGWLPYFELGMALYFILTMAYAAYSHIFGVLPFLSIFAVGYG
                     YVGILSFFQGASLKAKTPTPSEPQLNPAPQQ"
BASE COUNT          306 a          297 c          322 g          283 t
ORIGIN      
        1 gtgggggatc aacaccattg tatactctga gtaaaagggg tttagcgtgc ggcggcttga
       61 cgtcgcggtt ctattctcaa cgcgacgtca agctgcccgc tccaacgctt attgctgtgg
      121 tgccgggttc aattgcggtt ctgatggggt tggggttttc gctttcaggc tggcaccctg
      181 gaagaacgac agaatcccaa cgtagccata tccaacggca aaaattgaga gaaagggcag
      241 aactccaaag atatggctgt aggcggcata ggccatggtg aggataaagt agagcgccat
      301 tccaagttca aaatacggga gccatccccg gttgcggcgg tactttttgt tttgccagct
      361 atctttggca gtttcaattg catatttggg ggtgcggaca aaactggatt taatcccaaa
      421 gaccgcctcc atgacggctt tggcattact cattgacagt ccaacccccg tggccatcac
      481 cagataaatc atccacaact tggcgccttt tttcccgtgt aactccttga gggaaatata
      541 gtaaaacgag gtcacggaaa aggtcgagag gagcaggaag ggaatatcga gcagcatcat
      601 ctggaacagc ccttgattgt agcgcacgat caaaatcggg aagtgcagca ggttaaatac
      661 aatcatcaac gggtaggata agttgctcgt caaatggaaa aacatctcaa tccgcacgtg
      721 ccacgggagg tccgaattga gagtgcgttt aaacattttg aacgccacct gggtcaatcc
      781 tttggcccag cgtcgctgtt gagctttgaa ggcattgatt tcaaccggga gttcagccgg
      841 gacatcttcg tccatcacat agacaaattt ccagccgagc atttgtgccc gatagctcag
      901 gtctgtgtct tctgtcagcg tgtcatgctg ccagccacca gaccattcga tagcctgacg
      961 tcgccacata ccggcggtgc cgttaaaatt gaaaaagccg ccggaccggt tgcgcgaggt
     1021 atgttcgatc acaaaatgcc cgtcgagcag cacgctctgg atccgggtca ggacgttgta
     1081 atcggcattg atatggctcc agcggaactg caccacgcca actttttctt cggtgaagta
     1141 atgcaccatt ttgcggatgc aatctgggcg tgggcgaaaa tcagcgtcaa aaatggcaac
     1201 cagttcac
//
LOCUS       DAOQDO010002315         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149538_length_1208_cov_2.018213, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002315 DAOQDO010000000
VERSION     DAOQDO010002315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149538_length_1208_cov_2.018213"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /gene="tssI"
                     /locus_tag="PKZ53_26900"
     CDS             complement(<1..960)
                     /gene="tssI"
                     /locus_tag="PKZ53_26900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNJ44142.1"
                     /translation="MTYTQDNRLIQILTPLGKDVLLLRGYTAEEGISIPFQFNFRFYS
                     ENYGIPLTAIMGKKITLKIILADKKERYLNGIVNSFSQSGSSSTFAYYEATMVPWLWL
                     LTRTSDCRIFQDKSVPDIIEQVFKDLGFTDFKFRLYGEFVPRHYCVQYRETDFNFVSR
                     LMEEEGIFYFFEHEKDKHILILANHPNEFKPTKYQPEADYGRTEGQRQTEDVVTDWIT
                     NLEIRPGKYTHTDFNFEQPSLDLRSQITGKDPLQFELYDYPGEYQTRDEGDRLAGLRI
                     QEQDLPLQVITGGSICRGFMSGFRFDLKQHYRRDFNRAYVITMM"
     gene            complement(1016..>1208)
                     /locus_tag="PKZ53_26905"
     CDS             complement(1016..>1208)
                     /locus_tag="PKZ53_26905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44143.1"
                     /translation="GPSVTRLLIQAMASPMHLTGILGTAMAATRPVRRDREREISTFQ
                     SRTRIRKLHQDRTDQLGGF"
BASE COUNT          309 a          284 c          297 g          318 t
ORIGIN      
        1 catcatcgta atcacatagg cgcggttgaa atcccgccgg taatgctgtt ttaaatcaaa
       61 ccgaaacccg ctcataaatc cacgacaaat gcttccaccg gtgatgacct ggagcggcag
      121 atcctgttcc tgaatccgca acccggccag gcgatctcct tcatcacggg tctggtattc
      181 acccggatag tcatacaact caaattgaag tggatctttg cccgtaatct ggctgcgtaa
      241 gtccagcgac ggttgttcaa agttaaaatc agtatgggtg tattttcccg gtcgaatttc
      301 aaggttggtg atccagtcag tcaccacgtc ttcggtttgg cgctggcctt ctgtccggcc
      361 atagtcggct tcaggctgat atttcgtcgg tttaaattca ttgggatggt tggccaggat
      421 caggatgtgt ttatcctttt cgtgttcaaa gaaatagaaa attccctctt cctccatcaa
      481 tcgcgagaca aaattaaagt cggtttcacg gtattgaaca caatagtggc gtggaacaaa
      541 ttcaccatag agccgaaatt taaagtccgt aaaccccaaa tctttaaata cctgctcaat
      601 gatatcgggc actgatttgt cctggaaaat ccgacaatca ctggtgcggg tcaacaacca
      661 gagccagggc accatggtcg cttcatagta ggcaaaagtt gacgaagaac cactttgcga
      721 aaaggaattg acgatgccgt tgagatagcg ttctttttta tcggccagta tgattttcag
      781 agtgattttt ttccccataa tcgccgtgag gggaatgcca taattttcag agtaaaaccg
      841 aaaattgaac tggaagggaa ttgaaatgcc ttcttcggca gtgtatccgc gaagcagcaa
      901 gacgtctttc cccaacgggg tgagtatttg aatgagccga ttgtcttgtg tgtacgtcac
      961 agtgtcatgc ctcaaaagcc aggtgaaagt tcaggggttc gcggtgccat cagtgtcaaa
     1021 atccacccaa ttgatcagtt cggtcctgat gaagttttct gatacgggtt cgactttgga
     1081 aggttgaaat ctctcgttcg cggtcacgtc gaacggggcg agtggcggcc atcgccgtgc
     1141 ccaaaattcc agtcaagtgc atcggcgagg ccattgcctg aatcagtaac cgggtcacgg
     1201 atggccct
//
LOCUS       DAOQDO010002316         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149680_length_1207_cov_2.881076, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002316 DAOQDO010000000
VERSION     DAOQDO010002316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149680_length_1207_cov_2.881076"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            802..1005
                     /locus_tag="PKZ53_26910"
     CDS             802..1005
                     /locus_tag="PKZ53_26910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44144.1"
                     /translation="MNKIKQLRLWIYLIGIFLGAGLASMVNVPSIEGRQSGGPPATFF
                     TRKNVRKPRPQPRSGRAGMNRIR"
BASE COUNT          339 a          256 c          296 g          316 t
ORIGIN      
        1 tcctgagtct ttattttggg aagtctcaga aatcattgag gaagttggtt ttattttctg
       61 tgtgttccgt gtattccgtg gtttttttat ctgaaattct acaccagtac tgctctgttt
      121 ccccatatcg ctctggtttc cagagaacgg attgcattgg aatcgaatcc tcgtattcag
      181 cgtgtcaaat tcagactggg ttgaaatctg gtgtcgaaaa ctgggtgggt ttttcttttg
      241 gataccagag gctggagata tcaataacca tatgaaaagt aatgatttaa aagtatctca
      301 tatcataatt gtaatatatt tcctggttgg atgagttggt tagcctgaca ttgcaatcgt
      361 gacacgaaat ttcagaaata ggaaaccgcg atacttgaaa aggtttgatc cgctcgagga
      421 accgttctga acaattctgg gcacctgcga gtcaccgttt cgccatcaga cgtcaccgtg
      481 gactatgtgc gggccttctt gcctggcgac ggaaccaacg gacagattgg tcattcttat
      541 aaagttgcgg caccctccgc cgcaaagtga agaatgaaga attgagaatg aagaatgaag
      601 aatagacact atcggagata aaaccgggcc atcgaaaaat ggatggcgtc aagtcagtac
      661 cacccgcgtg agcgggtggg ttgccactgt gaggaagctc cactttggga caatggtgaa
      721 ccccgataat gtcgaatgaa gaatggaatc aatgtcttca gctctggtct tttcagccca
      781 acaccataca aaggcttcga tatgaataaa attaaacaac ttcgcttgtg gatctacctg
      841 attggaattt tcctcggagc tggtctggct tcaatggtga atgttccatc tatcgagggc
      901 cggcaaagtg gtggcccgcc cgccaccttt tttaccagaa aaaatgtgag aaagccccgt
      961 cctcaaccgc gaagcggtcg ggcggggatg aatcgcatcc gctgaaaaaa aacagcctga
     1021 atgaccttga acaaagtaat aaatcttgcc gaaaacggtg ggttggtgct agagttaaat
     1081 aatgaaaaag aaagcagata aacccaaaca accccaaacg ccatcctttg tcgccacgtt
     1141 tgaattggag gtggattcag ccgggttcgc gcaattggag aagcagctca atgcctatcg
     1201 ccaagtg
//
LOCUS       DAOQDO010002317         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149760_length_1207_cov_2.131944, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002317 DAOQDO010000000
VERSION     DAOQDO010002317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149760_length_1207_cov_2.131944"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKZ53_26915"
     CDS             <1..234
                     /locus_tag="PKZ53_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNJ44145.1"
                     /translation="GIWWVQLILNCLWSWLFFGLHQPKLAFVAIIVLWVSILANVIVF
                     GEIKPSAGLLLVPYLCWVGFAGWLNFMFWQLNP"
     gene            250..615
                     /locus_tag="PKZ53_26920"
     CDS             250..615
                     /locus_tag="PKZ53_26920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44146.1"
                     /translation="MKTLLLVYNAATGLGNALLDYAHKVLSPATYPCHLCALTHHHFG
                     PTQAFQEFLKTSQFKLEILHKDEFESAYQQSIVYPAILLKSENGQLQLIFSSDQIRTF
                     TTFEELRSALGELDFDQFT"
     gene            627..1094
                     /locus_tag="PKZ53_26925"
     CDS             627..1094
                     /locus_tag="PKZ53_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ44147.1"
                     /translation="MPAFEVSIAIESATSPEVIRLIQELDHYLTALYPAESNHLLSIS
                     ALNDPAVTLLVARYDGKAVGCGALLRTAPGFGEVKRMYVDPNRRGLGLGKKLLAAIEE
                     TARQAGIHTLQLETGIHQPEAIGLYIKNGYREIEPFREYQPDPLSRFFERKLG"
BASE COUNT          280 a          293 c          316 g          318 t
ORIGIN      
        1 ggaatttggt gggtacaact gattctcaat tgtctgtggt cctggctgtt tttcggtctt
       61 catcagccga aactggcgtt tgtggcaatt attgtgctgt gggtcagcat cctggccaat
      121 gtgattgttt ttggggagat caaaccatct gccggactgc tcctggtgcc gtatctctgc
      181 tgggttggat ttgctggctg gcttaacttt atgttctggc aattaaaccc ttaaacctga
      241 aggagcaccg tgaaaacact ccttttggtt tataatgccg ccaccgggct ggggaatgcg
      301 ctccttgatt acgcccataa agtgttgtct ccggcgacgt acccatgtca tttgtgcgcc
      361 ttgacgcatc atcattttgg ccccacccag gcttttcaag aatttctcaa aaccagtcaa
      421 ttcaagcttg aaatccttca caaagatgag tttgaatcag cctatcagca gtcaattgtg
      481 tacccggcca ttttgctgaa atcagagaat ggccagcttc aactgatttt ctcatctgac
      541 cagattcgca ccttcacaac ttttgaagaa ctccgaagcg cgttgggtga gcttgatttt
      601 gaccaattta cctgaccaca ccgcctatgc cagctttcga agtatccatt gcgattgaat
      661 cagccacctc gcccgaagta atccggttga ttcaagaact tgaccactat ctcacggctt
      721 tatacccggc tgagagtaac catctccttt caatttccgc cttaaacgac ccggcagtaa
      781 cgcttttggt cgcaagatat gacgggaaag cggttggttg cggggcgctg ctgcggacgg
      841 ctccgggctt tggtgaggtg aagcggatgt atgtagaccc aaaccggcgt ggtttggggc
      901 ttgggaaaaa acttctggct gcgattgagg aaacggctcg tcaagcgggc attcacaccc
      961 tgcaacttga aacgggcatt catcaacccg aagcgattgg cctctatata aaaaatggct
     1021 atcgcgaaat cgagcccttt cgtgagtacc agcctgatcc gctcagccgg ttttttgaac
     1081 ggaaactggg gtgaagaaac cagggctcag ggcctggggc tgaagaagtc gggctgaaga
     1141 agtcgggctg aagaagtcgg gctgaagagg tcgggctgaa gaagttggac tcaggatatt
     1201 gaacccg
//
LOCUS       DAOQDO010002318         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_149984_length_1206_cov_2.329279, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002318 DAOQDO010000000
VERSION     DAOQDO010002318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149984_length_1206_cov_2.329279"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKZ53_26930"
     CDS             complement(<1..315)
                     /locus_tag="PKZ53_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44148.1"
                     /translation="MTPDQAVFAPEGTHVPDGRHPVARWLCEDENAKEILEKVLEVKP
                     LDDEMWINALYYAVPQHSSWELPSDNRWRTFWRLLRSAPENARSRFVANDNRVSLLGI
                     LFQ"
     gene            487..903
                     /locus_tag="PKZ53_26935"
     CDS             487..903
                     /locus_tag="PKZ53_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44149.1"
                     /translation="MKKLNGVSDELTEVRAAFEVWRGERRGRQPIPARLWRAAVGLLD
                     SYPISVVARELRLNPTGLRQRSRSAESGTMTTRFVEVPSRGGTPVPVGVTTPPKTTDA
                     EIRLSIERRDGHRLSLVLPTAQGDQVERLWTAFVKG"
     gene            908..1177
                     /gene="tnpB"
                     /locus_tag="PKZ53_26940"
     CDS             908..1177
                     /gene="tnpB"
                     /locus_tag="PKZ53_26940"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HNJ44150.1"
                     /translation="MIQLVPHMRFLIAVEPVDFRRGIDALAAVCRQRLAQDPFGGTLF
                     VFRNRSGTAIKLLVYDGSGYWLCLKRFSRGKLAWWPTAESQRVVG"
BASE COUNT          265 a          330 c          358 g          253 t
ORIGIN      
        1 ctgaaacaat atacccaaca ggctcacgcg attatcattt gccacgaagc gggatcgtgc
       61 attctcgggg gcagaccgca acaacctcca gaaagtgcgc cagcggttat ccgagggcaa
      121 ctcccaagac gagtgctggg gaactgcgta atacagtgca ttaatccaca tttcgtcatc
      181 tagcggcttg acctcaagca ccttttccag aatctccttt gcattctcat cttcgcacag
      241 ccaccgggcg accggatgcc gaccgtccgg aacgtgagtt ccttccggcg caaagaccgc
      301 ttgatctgga gtcactaaac aatttgcgtc cgatgggatg atagccagag ttgggcgagt
      361 ttctgcccat tcacgtccct tacaaccatt cttatagtgt cctttcggtg gtgaggcgtg
      421 atttgaaacc ggaaaattga gtaaggtgac ctccgcaacc aatatctaaa cctggaggaa
      481 gactcaatga agaagctcaa tggtgtatct gatgaattaa ccgaggtccg ggcggcattt
      541 gaagtctggc gtggggaacg acgaggacgg caaccaattc cagccaggct gtggcgagcg
      601 gcggtgggac tgctggacag ctatccaatc agcgtggtgg ccagggaact gcgactcaat
      661 ccgacgggat tgcgccagcg aagccgaagc gcggagtcag gtacgatgac gacgcggttc
      721 gttgaagttc caagccgtgg ggggactcca gttccggtgg gtgtcaccac gccaccgaaa
      781 acgaccgatg cggaaatccg gctcagcatc gagcgccgtg acggtcaccg gttgtcactg
      841 gtgctcccaa cagctcaggg ggatcaggtt gaacggctgt ggacagcttt tgtgaagggg
      901 tgagccaatg atccaacttg tccctcacat gcgatttctc atcgccgtcg agccggtcga
      961 tttccggcgg ggaattgatg cgctggcggc agtctgccgc cagcgattgg cacaggaccc
     1021 cttcggcggc acgctgtttg tttttcgcaa ccgctccggg acggccatta aacttctggt
     1081 gtatgatggc agcggctatt ggctgtgcct caagcggttt tcgcgtggaa aactggcctg
     1141 gtggccaacc gccgaaagtc agagggtggt cggataggag cagcccattg ctggaccgcc
     1201 ccctcc
//
LOCUS       DAOQDO010002319         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150203_length_1205_cov_2.595652, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002319 DAOQDO010000000
VERSION     DAOQDO010002319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150203_length_1205_cov_2.595652"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>1205)
                     /locus_tag="PKZ53_26945"
     CDS             complement(147..>1205)
                     /locus_tag="PKZ53_26945"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNJ44151.1"
                     /translation="VCISASIGGATSQDLKTGFLVGATPKYQQMGLMIGVTGSAIAIG
                     WVLFFLNESATIHFPKDFPQYTATSNFVGTQAMPGGKSYRIHSIPVATNGILPGRYLV
                     DESGKIRYSIDPGVGGRESTQLIKTSGKVAENAKPMGTMRGLDGVEYQHVAVAAGEVE
                     TQYLVDEGGQIQYKFEPRKSRYDAPKATLMALIIDGILTQKLPWSLVLLGVFISITLE
                     LCGVAALPFAVGLYLPFSTSSPIFIGGVVRYLVDLILARQGKAAATEAEAESGNGVLF
                     SSGLIAGGAMGGLLVAALNAFKIGEHALAEKINVAEHFEWLEHGTYADLTAIVFFALI
                     GIVLWKVGQQTSPNKVTE"
BASE COUNT          311 a          327 c          297 g          270 t
ORIGIN      
        1 gtcaatccgc caacccacag aagtatcagg agcgcaacaa aaaagcagtt gagtgtgtgt
       61 tttcgaagca tatgtttgga gattcagggt tcagggttca gggttcaggg ttcaggattc
      121 aggatcttcg aatcaatgtt accgggtcat tctgtcactt tatttggtga cgtctgctgt
      181 ccaactttcc agagcacaat gccaatcaag gcaaagaaga caatggcggt caggtcagca
      241 taggtgccgt gctcaagcca ctcaaaatgc tcggccacat taattttttc agccagtgcg
      301 tgctcgccaa ttttaaaagc attgagtgcg gcaaccagca atcctcccat tgcccccccg
      361 gcaatcaacc cggagctaaa caaaacacca ttccctgatt cagcttcggc ttctgtggca
      421 gccgctttgc cttgccgggc caggatcaaa tccaccagat agcgcaccac cccaccgata
      481 aaaatcggag acgaggttga gaacggcagg tacaacccaa cggcgaacgg aagggcggcc
      541 accccgcaga gttccagggt aattgagata aagacgccca gcaaaaccag gctccaggga
      601 agtttttggg tcaaaattcc atcaatgatc agtgccatca gggtggcttt gggggcatcg
      661 tagcgtgatt tgcgcggctc aaatttgtac tgaatctgtc cgccttcatc aaccagatat
      721 tgggtttcaa cttccccggc ggcaacggcc acatgttgat attcaacccc atccaggccg
      781 cgcatcgtcc ccattggctt ggcgttttca gcgactttcc ccgaggtttt tatcagttgc
      841 gttgattcgc gaccgccaac ccctgggtca atcgaatagc gaattttgcc ggactcatca
      901 accagatagc ggcccggcaa aataccgttg gtggcaaccg gaatgctatg aatgcgatag
      961 ctcttgccac ccggcatcgc ctgtgtacca acaaaatttg aagtggcggt gtactgagga
     1021 aaatcctttg gaaaatggat cgtggctgat tcattcagga aaaagagcac ccagccaatg
     1081 gcaatcgcgg aaccagtcac gccgatcatg agccccattt gctggtattt tggtgtggct
     1141 ccaaccagaa aaccagtctt cagatcctgt gacgtggccc caccaatcga agccgaaata
     1201 cacac
//
LOCUS       DAOQDO010002320         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150288_length_1205_cov_1.990435, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002320 DAOQDO010000000
VERSION     DAOQDO010002320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150288_length_1205_cov_1.990435"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1205
                     /locus_tag="PKZ53_26950"
     CDS             141..>1205
                     /locus_tag="PKZ53_26950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ44152.1"
                     /translation="MDIQNTDSEIGFEALGLSLPLLKALQEVGYEAPTPVQLQTIPAL
                     KEGADVVGQAQTGTGKTAAFALPILDSLTLSTPVALQALVLVPTRELALQVAEAIHIY
                     SKYIGAVQVLPVYGGQPIQRQSRRLELGVHIVVGTPGRIMDHLRRGTMAFDSLKTVVL
                     DEADEMLKMGFLEDVEWILEQAKGRAQTALFSATMPTEVRKIADRYLNKAINVEIKRK
                     TLTVEGIEQRYLNVSIPNKLDILTRILEAEQGTATLVFTHTKIEAATVTEKLQARGYA
                     AEAMHGDMSQIHRESVIRRLRAGQVEIVVATDVAARGLDVDRISHVINYDIPYDPESY
                     VHRIGRTGRAGRQGKAILFVT"
BASE COUNT          296 a          314 c          309 g          286 t
ORIGIN      
        1 atcccttcga ttgccccaag cccactgtct tcctccccag gctctgtcag agtttcctag
       61 attggcaact cgtgctatgt attcaatgca ctcttaaatt ttcatatttg aagaaagtcg
      121 aacgtagttc agaaagaaac atggatatac agaacacaga ttcagagatc ggttttgaag
      181 cccttgggct gtcattgccg ttgcttaaag ccttacagga agttggttac gaagctccca
      241 cccccgtcca attgcaaacc atcccagctt tgaaagaagg cgctgatgtc gttggtcagg
      301 cacaaaccgg aaccggaaaa accgccgctt ttgccttacc gattttggat tcgctcactc
      361 tttccactcc ggttgccctt caggcactgg tgctggtccc gacccgtgaa ctggctcttc
      421 aggttgctga agccattcat atttactcaa aatacatcgg tgccgtgcag gtccttcccg
      481 tgtatggcgg acaacccatc cagcggcaat cacgccgatt ggaactgggt gtccatattg
      541 tggttggtac acctggtcgg attatggacc acctgcgccg tgggacgatg gcgtttgatt
      601 cgctcaagac ggttgtcctc gacgaagctg atgaaatgct gaaaatgggc tttttggaag
      661 acgtggaatg gattctggaa caggccaaag gccgggccca aaccgcgcta ttttcagcca
      721 ccatgccgac tgaagtccga aaaattgcag atcggtattt gaacaaagcc atcaacgtcg
      781 aaattaaacg aaagaccctg acggttgagg gcattgaaca gcgctacctc aacgtttcaa
      841 ttccgaataa gctcgatatc ttgacccgaa ttctcgaagc cgaacaagga acggcgaccc
      901 tggtgtttac ccacaccaaa attgaagcgg ccacggtgac tgaaaaactc caggctcgcg
      961 gatatgctgc cgaagccatg cacggtgata tgagccagat ccaccgcgaa agcgtgatcc
     1021 ggcgattgcg ggccgggcag gttgaaattg tggtggcgac tgatgtcgcg gcgcgcgggc
     1081 tggatgttga tcgtatctcg cacgttatca attatgacat tccgtatgac ccggaatcat
     1141 atgttcaccg aattgggcgc accggacgtg ccgggcgcca gggaaaagcg attttgtttg
     1201 tcaca
//
LOCUS       DAOQDO010002321         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150401_length_1204_cov_4.931245, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002321 DAOQDO010000000
VERSION     DAOQDO010002321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150401_length_1204_cov_4.931245"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..906)
                     /locus_tag="PKZ53_26955"
     CDS             complement(40..906)
                     /locus_tag="PKZ53_26955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44153.1"
                     /translation="MKNRLFQLFLLTACVSFSFSTPIFASEPTSTQVLYQKGQSFLSA
                     GQLTEAADTFKQLVRQSPKDFTAHHQLGITYFQLGRKSEAMTEIKQALQLNPRFAEGH
                     YNLGVIQDAAGNRREAQTAFKQAVTINPEYTKALIGLGEVSMDLKEYPQALEAYQIAA
                     QQQPKNAEVFNQLGIAHRALNQADEAVAAFRTAATLSPNDPGAHNNLGVALFQAGHIQ
                     AAIDEYKKAIALKSDYQEAAYNLGLARQVMDSPAPTVAQYRRLDSIHTRITQRLFGLQ
                     YTDRVGSPTFQR"
BASE COUNT          283 a          272 c          312 g          337 t
ORIGIN      
        1 tcatccctca tcccttctga aaacccggaa ccctgtagtt caccgttgaa atgtcgggga
       61 tcccacgcga tcagtgtatt gcaagccaaa gagccgctgt gtgatgcggg tatgaatcga
      121 atctaaccga cgatattgag ccacggttgg ggctggcgaa tccatcacct gacgggccaa
      181 tccgaggttg taggccgctt cctggtaatc agatttcagc gcaatcgctt ttttgtattc
      241 atcaatcgct gcctggatgt gaccggcctg gaacagtgcc acgcccagat tgttgtgcgc
      301 acctggatca tttggtgaga gcgtggcggc tgttcgaaat gctgccacag cttcatcagc
      361 ttgattgagg gcacgatggg caattcccag ttgattaaac acttcggcat tttttggttg
      421 ttgttgagcg gcgatttgat aggcttcgag cgcctgtgga tactctttga gatccattga
      481 aacttctccc aacccaatca atgcctttgt gtactctgga ttgatggtta cggcctgttt
      541 aaatgctgtt tgggcttcgc ggcggttccc ggcggcatcc tggatgacac ccaaattgta
      601 gtgaccttcc gcaaaacggg ggttgagctg gagtgcctgc ttgatctcgg tcattgcttc
      661 ggacttacgt cccagttgaa aataagtaat tcccagttga tgatgggctg tgaaatcttt
      721 gggcgactgg cgaaccagtt gtttaaaggt gtcagcggct tcagtcagtt ggccagccga
      781 taaaaacgat tgtccttttt gataaagaac ctgcgttgag gttggttctg aggcaaaaat
      841 tggggttgaa aatgagaatg aaacacaggc ggtcagcagg aacagttgga acagtctatt
      901 tttcatggcg aactcctttt tgttatggaa cgttattggc gatgcttacc tgagagaacc
      961 tcctccccta acagatcagc ttgaaacatc atgaccgtga cccattgacg ttcggtggtt
     1021 ctcttcgacg caaaccctag ttcaaccgac tctcaaaagt cctttcacgt tctgacattg
     1081 cagggttaca ttttctgaaa aagtcttaca tcggtctaag tttatgaaag aaaaatactt
     1141 atatgaattc ggggagtttc agttagatac catgcggcgg gtactgctgc gtgatggaaa
     1201 gaat
//
LOCUS       DAOQDO010002322         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150415_length_1204_cov_3.539600, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002322 DAOQDO010000000
VERSION     DAOQDO010002322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150415_length_1204_cov_3.539600"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1204
                     /locus_tag="PKZ53_26960"
     CDS             <1..>1204
                     /locus_tag="PKZ53_26960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ44154.1"
                     /translation="SGIYQNANRLIEETLGCRLNGLSVKEIIEALIEDPETRLKAAEF
                     VARGTTEWEYFPIKTRNGIKLDTEWKISRLPNGLNMGIGRDVTEQYQALNQQKQLEEQ
                     MWQAQKLETIGVLAGGIAHDFNNLLTAMLGNVGLALRIIPPDSPAVRKMKEVELAIQR
                     AADLTRQLLAYAGKGKYVVEPIDLSGLITKMAHLFQTIVSKKAYFSLNLVTDLPPIQA
                     DASQIQQVIINLLTNASEALEEKEGEIQLSTGLCTLTDQEIKSLFFYGNLRTGEYLFL
                     QVKDSGVGIPPDQVQSIFDPFFSTKFTGRGLGLAAVLGIVRSHNGGIQVYSQPGEGTE
                     VTIYFPKYEIAFETIRSSKTPTFDWQSSGTILVIDDEAEVRMLLQDVLEELGFDVLLA
                     QNGLEGLTM"
BASE COUNT          324 a          261 c          323 g          296 t
ORIGIN      
        1 agcgggatat atcaaaacgc caaccgattg attgaagaaa cgcttggctg tcgcctgaac
       61 gggttatcgg tcaaggaaat tattgaggcg ctcattgaag atccggaaac cagactcaaa
      121 gcggcagagt ttgtggcccg agggacaacc gagtgggaat atttcccaat taagactcgg
      181 aatggcatca agctggatac ggaatggaaa atctcaaggc tccccaacgg tctgaatatg
      241 gggattggcc gggatgtgac cgagcaatat caagccctta atcagcaaaa acaacttgaa
      301 gaacaaatgt ggcaggctca aaagctggaa acgattggtg tgctggctgg tggcatcgct
      361 cacgatttta acaatctgtt aaccgcgatg ctgggcaacg taggattggc gctgcgcata
      421 atccctccag attcgcctgc cgtcaggaaa atgaaggaag ttgaactggc gattcaacgg
      481 gcggctgatt taacccgcca gttacttgcc tatgccggaa aaggaaagta tgtcgtcgaa
      541 ccaattgacc tttcgggatt gatcaccaaa atggcgcatt tgtttcagac gattgtttct
      601 aaaaaagcct atttttcact gaatctggtt acggatttgc cgccgattca ggcagatgcc
      661 tcacaaatcc aacaagttat catcaattta ttgaccaatg cttcagaggc gctcgaagaa
      721 aaggaaggcg aaatccagct cagtaccggt ctgtgtacct tgaccgatca ggaaatcaag
      781 tcgttgttct tttatgggaa tttgcggact ggggaatatc tctttctcca ggtcaaagac
      841 tcaggggtgg ggattccccc agaccaggtt caaagtattt tcgatccgtt tttttcaacg
      901 aaatttactg ggcgcggtct ggggttggcc gcagtactgg gaattgtgcg cagtcacaat
      961 ggcgggattc aggtgtatag ccagccgggc gagggaaccg aagtgacgat ctattttcca
     1021 aaatatgaga ttgctttcga gacgattcgt tcgagcaaaa cccccacctt tgattggcaa
     1081 agttccggaa cgatccttgt gattgacgat gaagctgagg ttcgcatgtt gctccaggat
     1141 gtccttgagg aactggggtt tgatgtcctg ctggcacaaa atggcctgga aggactgact
     1201 atgt
//
LOCUS       DAOQDO010002323         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150430_length_1204_cov_3.009574, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002323 DAOQDO010000000
VERSION     DAOQDO010002323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150430_length_1204_cov_3.009574"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKZ53_26965"
     CDS             complement(<1..332)
                     /locus_tag="PKZ53_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44155.1"
                     /translation="MPDPTFAASIAESSPKDLVTGDELSMLLGNKLGAVAKNPFSGPE
                     ATPVEPFVPAAVAPGSKKRQRVIPYNFRRPDRVSKEQIHSLYLLHDNFARNVSSNLGL
                     MLRAITEVT"
     gene            complement(451..1179)
                     /locus_tag="PKZ53_26970"
     CDS             complement(451..1179)
                     /locus_tag="PKZ53_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNJ44156.1"
                     /translation="MEAHLPQVKWIAERIAARLPATVLLEDLISAGVIGLLDAVDKFD
                     PARGVLFKTYAEVRVRGAILDSLRHLDWAPRSIRRQSREIESIYARLEATHGRPAEEE
                     EVATAMGVPLNEFQVLLGTIRSLTLTDFGDEDDPEDSHLAISRIPDDPAHSPLVVYEQ
                     TERKERLAQAIDNLPERERQVVALYYLEELTLKEIGAVLGITESRVCQIHTQAILHLR
                     TTLNQTKDDPSGSRPVRSRVCQSR"
BASE COUNT          265 a          316 c          330 g          293 t
ORIGIN      
        1 gtgacctcgg taatggctcg caacatcaac cctaaattgg aagagacatt gcgggcaaaa
       61 ttatcgtgca gcaggtacag cgagtgaatc tgttcttttg aaacccgatc tggccggcga
      121 aaattgtatg gaataacccg ctgccgcttt ttcgagccag gtgcgaccgc tgctggaaca
      181 aagggttcaa ccggggttgc ctcgggtccc gaaaatggat ttttggccac cgccccaagt
      241 ttgtttccca gcaacatgga taactcgtca cctgtcacca ggtctttggg cgaagattct
      301 gcgattgacg cggcaaaagt tgggtctggc atagtcctcc gggaaagacc gtttggatgg
      361 aatgaaattc ctggatgatg aaagggttaa acccttgaga cgcttgtatt ttccttagcc
      421 cccggcccca taccccagcc caaaatgctc ttatctggac tggcaaaccc gtgatctgac
      481 tggccgcgaa ccagatggat cgtctttggt ttgattgagc gtggtgcgca ggtggaggat
      541 tgcctgggta tgaatctggc acactctgga ttcggtgatc cctaacacgg cgccaatttc
      601 tttgagggtt aattcttcta agtaatagag tgcgaccacc tgtcgctcgc gctctggcag
      661 gttatcaatc gcctgggcta agcgttcttt tcgctcagtt tgctcataaa cgaccagcgg
      721 cgagtgcgcc gggtcatcag gaatgcgact gatggccagg tgtgagtctt ccggatcatc
      781 ttcgtctcca aaatcggtca gggtcaggct gcggatagtt ccgagcagca cctgaaattc
      841 attgagcggt acccccatcg ctgtggcgac ttcttcttct tcggcgggac ggccatgggt
      901 ggcttcaagc cgggcataga tgctttcaat ttcacgcgac tgtcgccgga ttgaccgcgg
      961 cgcccaatcg aggtggcgta aactatctaa aatcgcccca cggacacgaa cttcggcata
     1021 ggttttaaac aacacgccgc gagccggatc aaatttatca actgcatcga gtaacccaat
     1081 cacccccgca ctgatcaagt cttcgagcag cacggttgca ggcaatctgg cggcgatccg
     1141 ttcggcaatc catttgacct gcggaaggtg ggcctcaatg agtgtatctg tgttttgctg
     1201 gcgg
//
LOCUS       DAOQDO010002324         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150683_length_1203_cov_3.832753, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002324 DAOQDO010000000
VERSION     DAOQDO010002324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150683_length_1203_cov_3.832753"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..>1203
                     /locus_tag="PKZ53_26975"
     CDS             256..>1203
                     /locus_tag="PKZ53_26975"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ44157.1"
                     /translation="MQSRFRLFQRLQWQLTLAYVSVTVVVVLVVEILGWLLLGLAFFL
                     SFLWPQALAAESVKLTPQALVCFEHQPPDREQLKIWVTNLRAGLQNPQFRRDNGFGFS
                     VGAAQEVVLAVTDPEGTALASAPDLAQPVNLMDGIQPDERAVWEYARQGFADPEHLNV
                     RHPGGKVVVLAPIIDRNNQVHGLLYVRIVAPFHFRDFAFNLIQMTLYQNLVILLGALV
                     IGSVCGYLVGRRFSHRFQRFFIAAQAWSQGEFQTIIPDASTDEVGELAERLNLMAQDL
                     NQVVQLRERVTLLEERARLARELHDTVKQQLFATTMQLGA"
BASE COUNT          257 a          303 c          319 g          324 t
ORIGIN      
        1 gacaacctgt tattttgttc acgaaaggca tgatggtcga actcctgtag attgaattgg
       61 tttttgttga tttatcgtcg tgataccatc ctatgccgtt tccaaccggc acacatcggc
      121 tctgagatgg aatttttgcg accagaaact cagacttaag tcggagtcgg aaatcattct
      181 ggagacggtg gtgggaacat ttcgtcaccg atacaatggt gtcagtttcc attcttcttt
      241 tctcaaggga gccttatgca atctcggttt cgactcttcc aacggctgca atggcaattg
      301 acactggctt atgtcagtgt gacggtggtt gtggtcctgg tggttgaaat ccttggctgg
      361 ttgttacttg ggctggcctt ttttctttcg tttttgtggc cacaggcgct ggcggcagaa
      421 tcggtcaaac ttaccccgca ggcactggtc tgctttgaac accagcctcc tgatcgcgaa
      481 caactcaaaa tttgggtaac caatctacgc gctggactcc aaaaccccca atttcggcgt
      541 gacaatggat ttgggttcag cgttggcgcc gcccaggaag ttgtactggc ggtgacagat
      601 ccagagggca ccgccctggc cagtgcacct gacctcgctc agccagtgaa tttgatggat
      661 ggaatccaac ctgatgaacg tgcggtctgg gagtatgccc ggcaagggtt tgctgacccc
      721 gaacatttaa atgtgcgcca tccaggtggg aaagtggtgg tgctggcccc aatcattgac
      781 cgcaataatc aggttcacgg attgctctat gttcggattg tggcgccatt tcattttcga
      841 gactttgctt tcaacctgat tcaaatgacg ctttaccaga atctggtgat cctgcttggg
      901 gccttagtga ttgggtcagt atgtggatat ctggttggtc gccggttttc acaccgcttt
      961 cagcgatttt ttattgcggc ccaggcctgg agccagggag aatttcaaac cataattcct
     1021 gatgcctcta ccgatgaagt cggtgaactg gccgaacgcc tcaacctgat ggcccaggac
     1081 ctcaaccagg ttgttcaact ccgggaacgc gtgacgctgc tcgaagaacg cgcccgactc
     1141 gcccgggaac tccacgatac ggtcaaacaa caactttttg cgaccacgat gcagcttggg
     1201 gcg
//
LOCUS       DAOQDO010002325         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_150736_length_1203_cov_2.547909, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002325 DAOQDO010000000
VERSION     DAOQDO010002325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150736_length_1203_cov_2.547909"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..829
                     /locus_tag="PKZ53_26980"
     CDS             386..829
                     /locus_tag="PKZ53_26980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44158.1"
                     /translation="MGDSFDYLIRERVLCGIAANERFVVKAFSGGGGGSTRRGVPDPT
                     LVNNPTMASHKRGADPLRPRGGPLPAGVYTMMLPTRHPHLGLSVYLAPSPQNQMFHRD
                     GFFIHGRGPQGSDGCIVPDDPQEFHRLMNAISRAGGGILRVFLQY"
BASE COUNT          286 a          295 c          283 g          339 t
ORIGIN      
        1 gctcagggta ttaaacccgg tttcttccgc cccgagcttg cgagtcttca gccctgagcc
       61 ccgagcttgc gagtcttccg ccctgacttt gccatttgcc tttccagcca cttcactctt
      121 cacttttgaa ctcgctggtt tggcaaatgg cattattctt gtgtaacaac cgtgggcttc
      181 gcaccacggt aagttgttca agagccgtga tgcgaagccc acggtgaaca gaaccaacaa
      241 attttaagca ggccaggtca accaaccagc tatccgaatg gttattcttt ggattcaagc
      301 cctacctttg gcatatttat tgccgagggt ttatttcaaa agttcattta cttttgaaat
      361 taaattcgga ggaatttctt gatttatggg agattcattt gattatctca ttcgcgaacg
      421 agtgttgtgt gggattgcag ccaacgaacg atttgtggtg aaagcgtttt ccggaggtgg
      481 tggtggaagt acccgacggg gtgttcctga tccaacactt gtcaataacc cgaccatggc
      541 atcacataaa cgtggagccg acccacttcg cccacgaggt ggaccacttc cagccggagt
      601 ctatacgatg atgttaccaa ccagacaccc acacctcggg ttgagtgttt atttagcgcc
      661 ttcacctcaa aatcaaatgt ttcatcggga tggttttttt attcatgggc gtggtccaca
      721 aggaagtgat ggatgtattg ttcctgatga tccacaggaa tttcatcgcc tgatgaacgc
      781 catctcacga gcaggcggcg gaattctccg tgtattcctc caatattaat aaaaaccaaa
      841 tgtacctgaa catcagaaga ggactgtgat gaaacactgc gtactcatcg tctttctggg
      901 attgtttttt ggggggtgtc attctacaca accaggtgcg ccccaatcac ccagggactt
      961 agaaacgaat tccttggttt tgaaagaaat tgcccattca tccgcaccct taggggtttt
     1021 caattgtctg gcggtttctc ctgataacca atttgtgata gccgccggag aaaatccagt
     1081 cgtttacggg tgggaacttg ccagagggat tgaagttcct gccggaacct tctctggaca
     1141 ccatcaagca atcacatcac tggcgttttc atccgatggg agtcggattg tctcggccag
     1201 ttc
//
LOCUS       DAOQDO010002326         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151187_length_1201_cov_3.410995, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002326 DAOQDO010000000
VERSION     DAOQDO010002326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151187_length_1201_cov_3.410995"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..879
                     /locus_tag="PKZ53_26985"
     CDS             139..879
                     /locus_tag="PKZ53_26985"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNJ44159.1"
                     /translation="MMKELGQVCELVRYPVKSMAGSVTESAFLGWHGLDGDRRFAFRR
                     LGDDSGFPWLTASRVPELLLYQPIGLDERTGEPLPTHIQTPAGTQMELYSLELQREIA
                     ERFGSSVELMKLKHGIFDDAPLSVISVATIAGIGSEAGMHLDRRRFRANIVIETHDHE
                     PFLEDGWVRGTLVFGTTEPRPAVRVTARDVRCMMINLDPDTAEQNKQVMKTVVRLNQN
                     NAGVYGTVVQTGTIRVGEPVSLVWDSWG"
     gene            complement(993..1196)
                     /locus_tag="PKZ53_26990"
     CDS             complement(993..1196)
                     /locus_tag="PKZ53_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ44160.1"
                     /translation="MAALLAEPVDLCKSLRQARKDRGFSQIQLAEELGIGQAYLSKIE
                     SGRVSEKQIGPTLRAKFISWLRS"
BASE COUNT          267 a          321 c          344 g          269 t
ORIGIN      
        1 gcaatcaacc tggtcccatt ttcttcagcc ccaagcccct ggcagtggca tcgccgttgc
       61 aattgacact ccttatcgct atcgtcgctg aacctgagtt gttaaatcgc ttaccgtggc
      121 gtgacatgga gtgaccatat gatgaaagaa cttggacagg tttgtgaact ggtgcgatat
      181 cccgtcaagt caatggcagg gagtgtgacg gaatcggcat tcctcgggtg gcatgggctc
      241 gatggtgatc ggcgctttgc cttccggcgg ctgggagatg acagtggttt cccgtggctg
      301 accgcgagcc gtgttcccga actgcttttg taccaaccca ttggtctcga cgaacggaca
      361 ggcgaacccc tgccaactca tattcaaact ccagccggaa cgcagatgga actttacagc
      421 ctggaactcc agcgcgagat cgccgagcgg tttgggagca gcgtggaact catgaaactc
      481 aagcacggga tctttgatga tgccccgctc tcggtgataa gcgttgcgac cattgccgga
      541 attggtagtg aggcgggaat gcatcttgat cggaggcgct ttcgagccaa cattgtgatt
      601 gagacccacg accacgagcc gtttcttgag gatgggtggg tgagggggac cttggtgttt
      661 ggcaccaccg aaccacggcc agccgtaagg gtgacggcac gtgacgtccg atgcatgatg
      721 atcaaccttg atccagacac ggctgaacaa aacaagcagg tcatgaagac cgtcgtccgg
      781 ctcaaccaga ataacgccgg cgtctatgga acagtggtgc aaacaggtac tatccgcgtt
      841 ggcgaacctg tgagtctggt atgggattcg tgggggtgaa aactctgggg ctaagggctg
      901 aagaaccagg gctgagggta ttgaacccgt tttcttcaac cccaagccct tagccccaag
      961 ccctcagccc cagttcttca gccctggtac tttcagcttc ggagccacga aataaacttg
     1021 gcccgcaatg tcgggccaat ttgtttttca gagacccgtc cgctttcaat ttttgaaagg
     1081 taggcctgcc caattccaag ttcttcagcc agttgaattt gagaaaatcc gcgatctttt
     1141 cgagcctgcc gcaggctctt gcacaggtca accggttcag ccaggagcgc agccacgggg
     1201 g
//
LOCUS       DAOQDO010002327         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151440_length_1200_cov_3.691703, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002327 DAOQDO010000000
VERSION     DAOQDO010002327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151440_length_1200_cov_3.691703"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..>1200)
                     /locus_tag="PKZ53_26995"
     CDS             complement(173..>1200)
                     /locus_tag="PKZ53_26995"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ44161.1"
                     /translation="VFDRQSRAKAPAVTAHHVIAHYVRQTGLPETFLRDEILLQLSSV
                     DATLQSQVIGQPDACRLAAQVITTFKAGLNDPHRPVAVLLFCGPTGVGKTELAKAISR
                     YLFGSGQAAEQRLIRLDMSEYSGYGALDRLFTAPNGKPGQLIERIRRQPFSVVLFDEI
                     EKADEAVFDALLGVFDEGRLTDRFGRTTSFRNAVIVMTSNLGADLSPAIGYGASTTPP
                     YEKVAMSFFRPEFFNRLDAVVRFQALTPSMVLDITRKELSEISQREGFTKARLQLAWS
                     ETLVAQCAREGYDVRYGARPLQRTLESTVVAPLARYLLQHPKLRSKTLFLDWSTQTST
                     LTIVSNS"
BASE COUNT          316 a          300 c          316 g          268 t
ORIGIN      
        1 aggtgttgat gacttcatag ctataagttg gaatggctga tggttgaacg gttgtcgaga
       61 caacttttcc ctgagtcgtc aaagaaaaag tcagactgct tgcaataaac aaatagatca
      121 aaaatacaac gcgcatacgc ttttcccact gaaaataaag aaaggtgaag tgtcaggagt
      181 tggaaacaat cgttaaggta ctggtttgag tggaccaatc aagaaaaagt gttttggatc
      241 tgagtttcgg atgttgcaaa agataccggg ccagcggcgc cacaaccgtt gactcaaggg
      301 ttcgctgcag aggtcgggcg ccataccgga catcataccc ttcacgagca cactgtgcca
      361 ccagtgtttc tgaccatgca agctggagcc gggctttggt aaagccttca cgctgactga
      421 tttcgctcaa ttccttgcgc gtaatatcca gcaccattga cggagtcagc gcctgaaacc
      481 ggacgacggc atccaggcga ttaaagaatt cgggtcggaa aaacgacatg gcgacttttt
      541 catagggggg agtcgtcgag gcaccatacc caatggccgg tgatagatcg gcgcccagat
      601 ttgaggtcat gacaatcacg gcattgcgaa aactggtcgt tcgcccaaat cggtcggtta
      661 accggccttc gtcaaagaca ccaagtaacg catcaaaaac cgcttcatcg gctttttcga
      721 tttcatcaaa cagaaccact gaaaatggct ggcggcgaat gcgctcaatc aactggcctg
      781 gtttgccatt gggggccgta aacagccggt cgagcgctcc atacccgctg tattcgctca
      841 tatccagccg aatcagtcgt tgctcagccg cctgaccact gccaaataaa tatcgggaaa
      901 tggctttggc cagttcggtt ttgccaactc ccgtcggacc acaaaataac agtaccgcca
      961 ccgggcgatg tgggtcattg agaccggctt taaaggttgt aatcacctgg gctgccagcc
     1021 ggcaggcgtc cggttggccg atgacctgtg attgcaaagt tgcatccact gacgaaagtt
     1081 ggagcaggat ttcgtcccgc aaaaaggttt cgggtaagcc agtctggcga acatagtggg
     1141 caatcacatg gtgagctgtc accgccggag ctttggcccg actttgacga tcaaacacat
//
LOCUS       DAOQDO010002328         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151473_length_1200_cov_2.707424, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002328 DAOQDO010000000
VERSION     DAOQDO010002328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151473_length_1200_cov_2.707424"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKZ53_27000"
     CDS             <1..733
                     /locus_tag="PKZ53_27000"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNJ44162.1"
                     /translation="QWNPTTISFPAVHQDDQLLLTGQKLFVPDAETADVIICVARSND
                     QLVLIPVEKTSDGVQIKPMPAMDSTRKWYQVNFEGVSVPETVVLGADGNSQAALERAV
                     EVATIALCAEMVGGMQWVLETAVEYAKVRHQFGKPIGSFQAVQHQCADMLLMTESARS
                     ATLFAAWALSVNDPRATEAVSIAKAYCSDAYREVCHRGVQVHGGIGFTWEYDLQLYVK
                     RSKSSEAFFGDAIYHRERIAQLIIG"
     gene            819..1196
                     /locus_tag="PKZ53_27005"
     CDS             819..1196
                     /locus_tag="PKZ53_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNJ44163.1"
                     /translation="MTNNEISGAIVDAAMKVHSQLGPGLLESAYEKCLLYELHKRGLQ
                     AQAQVEVPVLYDEIKIDAGFRLDLLVENTVIVELKAVEAVLPIHHAQILTYLKLSGKP
                     IGLLINFNVQHLRNGIKRFVKKT"
BASE COUNT          309 a          282 c          327 g          282 t
ORIGIN      
        1 ccagtggaac ccaacaacca tttcattccc ggcggtacat caggacgatc agttgctcct
       61 cacaggtcaa aagctgtttg tgccagatgc agaaacagca gatgtcatta tctgtgtggc
      121 gcggtcaaat gatcaattgg tgctgattcc ggttgagaag acttcggatg gggttcaaat
      181 caaacccatg ccggcgatgg acagcacccg caaatggtat caggtcaatt ttgaaggcgt
      241 gtctgttcct gaaacggtgg tgcttggggc tgacggaaat tcgcaagcag ccctggaacg
      301 tgcggtcgaa gtcgccacta tcgcactgtg tgcggaaatg gttggtggaa tgcagtgggt
      361 gcttgagaca gcagttgaat acgccaaagt ccggcatcaa tttggaaagc cgattgggtc
      421 gtttcaggca gttcaacacc agtgcgccga tatgttgttg atgaccgaaa gcgcccgttc
      481 ggcgacactg tttgcggcct gggcgttgag tgtcaacgac ccacgggcaa ctgaagcggt
      541 ttctattgcc aaagcctatt gctcggatgc gtaccgcgaa gtttgccacc gtggcgttca
      601 agtccacgga ggaattgggt ttacctggga atacgacctc caactgtacg tcaaacgcag
      661 caagtcatcc gaggcgtttt tcggagatgc gatttaccac cgcgaacgaa ttgcccaact
      721 gatcattggc taagttgccg aattattgag ctggttagcc tcaatttttg aaccacagag
      781 gacacagagg agcacagagg aagaaggaag ataaagccgt gacgaataac gaaatcagtg
      841 gggcgattgt agatgccgct atgaaagttc attcgcagct tgggcccggg ctactggaaa
      901 gtgcgtatga aaaatgcctg ctctacgaac tgcacaaacg cggtctgcaa gctcaagccc
      961 aggtcgaagt tccggttctt tatgacgaaa tcaaaattga tgccggtttc cgccttgact
     1021 tactggtgga gaatacggtt attgtagaac tcaaagccgt tgaagccgtt ctgccaattc
     1081 atcacgctca aatcctgacc tatcttaaac tgagcgggaa accgattggg ttgttgatca
     1141 atttcaatgt ccagcacctg cgcaacggga tcaaacgctt tgtcaaaaag acctgatctt
//
LOCUS       DAOQDO010002329         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151482_length_1200_cov_2.582533, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002329 DAOQDO010000000
VERSION     DAOQDO010002329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151482_length_1200_cov_2.582533"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKZ53_27010"
     CDS             <1..1025
                     /locus_tag="PKZ53_27010"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNJ44164.1"
                     /translation="AVKERQGCAVYYAPGHDYNIEKLLDGMIAARRIYERDFGPLPYH
                     DLRLVEFPRLAEFAQGYPTTIPCSEGIAFLTRDDGAYVNTNAFTVAHEVAHQWFGNMV
                     VPGRSRGAAVLLEGLAEYAAGAMIEESVGEQAAKVFRRYEETAYLQNRQVDTEPTLVT
                     IDGSAVFDSVVSYQKAGLVFHMLEKLIGRESMKQALKTYVARFAWKDVHPTIHDLISI
                     FKQQVPDRSLDWFLNDWFFGVTLPDSRIVSAVVQIVGNEYHVEVTVENQGQGRVPVMV
                     EVITGNETDQAHFQSQSIQVMTEPGKPMTGIIVCSFKPEKIVLDRSFDWLDADRENNE
                     QSVFVP"
BASE COUNT          289 a          256 c          316 g          339 t
ORIGIN      
        1 atgccgtgaa ggagcgtcag gggtgtgcgg tatattacgc tccgggtcat gactataaca
       61 ttgaaaaact cctggacgga atgattgccg cccgtcggat atacgaacgc gacttcggac
      121 cactgccata tcacgatctt cggctggttg aatttccacg gctggcggag tttgcccagg
      181 gatatccgac gacgattcct tgctcggaag gcattgcatt tttgactcgc gacgatggag
      241 cgtatgtcaa caccaatgca tttactgttg cccacgaagt tgcccaccag tggtttggta
      301 atatggttgt gccggggcgg agcagaggcg ccgccgtttt gcttgaaggg cttgctgagt
      361 acgccgctgg ggctatgatt gaagagtctg taggtgagca ggctgccaaa gtttttcgtc
      421 ggtatgaaga aaccgcgtac cttcaaaacc gtcaagttga tactgagccg acattggtga
      481 ctattgatgg aagtgccgtt ttcgactcgg tggtgtctta ccagaaagca gggttggtgt
      541 ttcacatgct ggagaagctc atcggacgcg agtcaatgaa gcaggcactc aaaacctatg
      601 tcgctcggtt tgcctggaaa gacgttcatc cgacaatcca tgatctcatc tccattttta
      661 agcaacaggt tccagacaga tcgctcgact ggtttttgaa tgactggttt tttggcgtca
      721 cgttacctga cagccgaatt gtttcggcag ttgtccaaat tgtcgggaac gagtatcacg
      781 ttgaggttac cgttgaaaat caaggtcagg gaagagtacc cgttatggtc gaagtcatta
      841 caggaaatga aactgatcag gcacactttc agtcacaatc catacaggtt atgactgaac
      901 ctggaaaacc gatgactggt atcatcgttt gttcctttaa accagagaaa attgtgttag
      961 atcgatcctt tgattggctt gatgccgacc gcgagaacaa tgaacaatcc gtatttgtac
     1021 cttgagctac ttccactgga gattgattct gataagtagt tgatttattt cgtgtttaca
     1081 aattttttgg aattcgcagt caattttgcc tggatatttg actttgagca tttttgccaa
     1141 tgctgtgtgt ggtgtattgc tggagatttt cctggaaaac cgcatcggca tacgtggcat
//
LOCUS       DAOQDO010002330         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151929_length_1198_cov_4.734033, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002330 DAOQDO010000000
VERSION     DAOQDO010002330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151929_length_1198_cov_4.734033"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKZ53_27015"
     CDS             <1..298
                     /locus_tag="PKZ53_27015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ44165.1"
                     /translation="ESNVEIAESGLNQMSDPRQRPDGELLTKELNCVIHHALSLLDED
                     KRLIFSLQAFHQYSYEEIAEITGFTIPKIKTDLHRAKAEMRKRIRPYLGERHEL"
     gene            288..1169
                     /locus_tag="PKZ53_27020"
     CDS             288..1169
                     /locus_tag="PKZ53_27020"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ44166.1"
                     /translation="MNCDDYVLLLEEFVDGELGESDTKKVEVHLAACPACQKVLAELK
                     EEQAIFARYDTGLELTPNLWQGIEDRLKAEGSFGTSKPVEPQGWGPKIKDWFTRHFHT
                     PQLSPAFTMVVVVAAVGLTVLVMRPKPAEGPFSSVNPGINIGPGSSTHDEVVIASTPK
                     PEATAAVIQPAPPRPVKPAPPPPAAPAKREPSVQELIRDAESKYRAAITVLTRDVKKR
                     RTTMDPLLVARLDSTLEAIDHTIADTKLAVKMNPNDPVAAQYLLTAYAKKVEVLQEMA
                     SVASPATPTKKPVELRP"
BASE COUNT          324 a          330 c          297 g          247 t
ORIGIN      
        1 tgaatcaaat gtcgaaattg ccgaaagcgg attgaatcaa atgtctgacc cacggcagcg
       61 acccgacggc gaattgctca ccaaagaact caattgtgtc atccaccacg ccctttcact
      121 cctcgatgaa gacaaacggt tgattttttc actccaggca tttcatcagt atagttatga
      181 ggaaattgcc gaaatcaccg ggtttaccat tccaaaaatc aaaaccgacc ttcatcgggc
      241 caaagcggaa atgcgcaagc ggattcgtcc ttatctggga gaacgccatg aactgtgatg
      301 actacgtgct tttacttgaa gaatttgtgg acggggagct tggtgaatca gacaccaaaa
      361 aagtcgaggt tcatctggct gcctgtcctg cctgccagaa agttctggct gaattgaaag
      421 aggaacaggc aatttttgcc cgctatgaca ctggtctgga actcacacca aacctctggc
      481 aagggattga agaccggttg aaagcggaag gttcctttgg cacctcaaaa ccagttgaac
      541 cccagggctg ggggccaaag atcaaagact ggttcacgcg ccactttcac accccacaac
      601 tgagcccggc atttaccatg gtggtcgtgg tggcggctgt cggattaacg gtcctggtga
      661 tgcgaccgaa accggcggaa gggccattct cgtcagttaa tccagggatc aacattggcc
      721 ctggaagttc aacccacgat gaggttgtca tcgcttcaac tccaaaacct gaagccactg
      781 ccgccgtgat acagcccgca ccacctcgcc cggtcaaacc agcgcctccg ccgccagccg
      841 cacctgccaa acgggagcca agcgttcagg agttgattcg agatgctgaa tcaaaatacc
      901 gggccgcgat tacggtgcta acacgtgatg tcaagaaacg ccgaaccacg atggatccgc
      961 tccttgtggc tcgactcgac agcacccttg aagccattga ccacacgatt gccgacacca
     1021 aactggccgt caaaatgaat ccaaatgatc cggttgccgc ccaatatttg ctgacggcct
     1081 atgccaagaa agtcgaagtg ctccaggaaa tggccagtgt ggcatcgcca gccactccga
     1141 ccaaaaaacc ggttgaattg agaccctaaa atgaaacagg gttcagggtt cagggttt
//
LOCUS       DAOQDO010002331         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151943_length_1198_cov_3.524059, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002331 DAOQDO010000000
VERSION     DAOQDO010002331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151943_length_1198_cov_3.524059"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          316 a          287 c          262 g          333 t
ORIGIN      
        1 ccgcggctgg tttcccggtt gtaatgccat gacaatgatt cctgcttttt ataactgatc
       61 tggatttttt catccgttga caccatcccc gaatctcagt cagaggcctc agcccagagc
      121 tgtgccgtgg cgtaagcaca cgactcccat cagagaataa atccagaaat tgattgaaca
      181 aggtggacga tcaacaacgc ccgttcggat ttctcaccat aaagacgtcg ccccggccag
      241 tcctgtgact gggatcttga tccagatgca ctccgcctga aaccaattct gaattggctg
      301 agagtgagga attgaatggc tggtcaggtt gtgaatcgtg tcacctgtat acgaaagata
      361 ctgaccgttt cgttggtcag agccgatcta ttccgtgcat gagcacgtcc ccggtcggac
      421 ttaaatgtgg ctgtaacttt taagactctc caacatagtg gtgaatctgt tcaggtgtca
      481 aggaaaaaat aagagaaaaa tacaggacct ggatttgatt tttccatcca gactgaaaaa
      541 ggaagtgtcg ttttcatggc cagttctcaa ctcttttctg aaatatggtc agtttgatgt
      601 gggatcgaag tcaatcgggt accgtgattt gaagtggaac ctgattcagg aaaatggacc
      661 caaaccccaa ggcggatgat tcggtccatt ggtgctgaat cgggggaatg tccttcattc
      721 ggtagggcaa tccttgtgcc acattaaaaa ctggcaacga actcgtttgt tcaaataatt
      781 aaccccttga aaaacctgta cttaggctca actccaattc aggctcaccg ctcccagcag
      841 atatattttt caccttttgc tgaaatattt ttaaccaaaa ggtgaaaccg ggtaaaatat
      901 tgatttactg aaggatatcc aggttttatc ctctttgtgt ctttcctgtc tttcactttt
      961 tggttaaaac ctctccagat gaatgactaa cacctggttg ttttgagata tcgatgctca
     1021 ttcaacgggc gccatatcag gtaactggtc ccgccaaagc cacacggact aatttcaaat
     1081 caagacctgc catccagaac ccgctctgga tccaaccagg tatcactgcc cttcaacgaa
     1141 accagggctg tcaggacctt cagcttgttt tgagcgtctt ccgataatct catttcgc
//
LOCUS       DAOQDO010002332         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_151946_length_1198_cov_3.404199, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002332 DAOQDO010000000
VERSION     DAOQDO010002332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151946_length_1198_cov_3.404199"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1198
                     /locus_tag="PKZ53_27025"
     CDS             112..>1198
                     /locus_tag="PKZ53_27025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ44167.1"
                     /translation="MSTSRLPLLALFGAVGFVLCIACANVAGLFLARSLTRQREIAIR
                     QALGASRRQLLQMVLTESLMLSLLAGGLGIGVTFLGVETLVKLAPADIPRLAEVAIDP
                     QVLLFTFVISIVTGILFGIAPALHGSRQGIAQTLQESNHINSTGGVFPNRMRKALVVV
                     EVALAMVLLFGAGLFIRSFLNVLSVDPGFQSANLLVLRVFLDGGTYKEGRQGLVYYQN
                     LYERLQSLPGVTSVAATTVLPMSGVGIDFDRPVWREGEPEPVGVPRETSIRMVTPNYF
                     DTVGIPFRSGRAFTADDRPETPRVLIVNEALVRREWPDGNAVGKRLMVDYLGGKYPYE
                     VVGVVGDARYYGLKSTPQPEIFIPHAQN"
BASE COUNT          235 a          297 c          355 g          311 t
ORIGIN      
        1 ggcgacgttc gtcgtgccgc tgtccaccaa cgtcacgccg gcctggacca gcagttttcc
       61 aaaaccaatg ctggatggcg ggtttcgctg gttcggcttc aggatgaact ggtgagcacc
      121 tcacgcctgc cgctcctggc cctgtttggc gcggtcgggt ttgtgctgtg tatcgcctgt
      181 gccaatgtcg ccgggctttt tctggccaga tcattgaccc gacagcggga aattgcaatt
      241 cgtcaggcac ttggggcgtc gcgacggcag cttttgcaga tggttttaac cgaaagcctg
      301 atgttatcgc tcctggctgg tggccttggg attggcgtga cgttccttgg ggtcgaaacg
      361 ctggtcaaac tggcaccggc tgatattcca cgattggctg aagtagcgat tgaccctcag
      421 gtcttgctct ttacctttgt gatttcaatc gtcactggca ttctgtttgg aattgcccct
      481 gccctgcacg gaagtcgcca gggaatcgcc caaactttgc aggaaagcaa ccacatcaat
      541 tccacgggcg gtgtgtttcc aaaccgaatg cgcaaagccc tggtggtggt tgaagtggca
      601 ctcgcaatgg tactgctgtt tggcgccgga ctgtttattc gtagctttct caatgtgctt
      661 tcggtggatc caggatttca atccgcgaat ttactggtgt tgcgggtttt tctcgacggt
      721 ggaacctaca aagagggtcg gcaggggctg gtctactatc agaacctcta cgagcggttg
      781 cagagtcttc cgggtgtcac ttcagttgcg gcaaccacgg ttttgcccat gagcggagtt
      841 gggattgatt ttgatcgtcc ggtttggcgt gaaggagagc cggaaccagt tggagtccct
      901 cgcgaaacca gcatccgcat ggtgacacca aactattttg acacggtggg aattccattt
      961 cgttctggac gggccttcac ggctgatgat cgtccagaga cacccagggt gttaattgtg
     1021 aatgaagccc tggtccgccg ggaatggcca gatggaaatg ctgtgggaaa acgattgatg
     1081 gttgattacc tgggcggaaa atacccatat gaagtggttg gcgtggttgg agatgcgcgg
     1141 tattacgggt tgaaaagcac tccacagccc gagattttca ttcctcatgc tcaaaatc
//
LOCUS       DAOQDO010002333         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152000_length_1198_cov_2.306212, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002333 DAOQDO010000000
VERSION     DAOQDO010002333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152000_length_1198_cov_2.306212"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKZ53_27030"
     CDS             <1..313
                     /locus_tag="PKZ53_27030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44168.1"
                     /translation="IELGGNDGILLATYGTCLIYEKKYEAAVEKLERAVQLIPQNPAF
                     SYNLACAYARLNQKEKALSALEQAVRTGFRDKSGMLADPDLENIRSDAKFQTLANSIG
                     T"
     gene            512..1084
                     /gene="msrA"
                     /locus_tag="PKZ53_27035"
     CDS             512..1084
                     /gene="msrA"
                     /locus_tag="PKZ53_27035"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNJ44169.1"
                     /translation="MSSNPLLPSSANSITEVATLGGGCFWCLEAVFDQLQGVLHVESG
                     YSGGTVENPTYMQVCAEITGHAEVVQVTFDPAVISFQEILQIFFVIHDPTTFNRQGAD
                     IGTQYRSAIFYHSAEQKQIAETMIQELNAVGVYDRPIVTEVTAFTRFFEAEPYHQEYF
                     VRNPAQGYCQFVVSPKVAKFRKLFAEKLKT"
BASE COUNT          304 a          289 c          289 g          316 t
ORIGIN      
        1 gattgaactc ggcggcaacg acgggatatt gctggcaacc tatggcacct gtctgattta
       61 tgaaaagaaa tatgaagctg cggttgagaa gcttgagcgg gcggttcagt taattcccca
      121 aaatccagcc ttttcttaca atctcgcctg cgcctatgcc cgattaaacc agaaggaaaa
      181 agcattgagc gcgcttgaac aggcggtacg gacagggttc cgcgacaaat caggcatgct
      241 ggctgatcca gatttggaaa acattcggtc ggatgcaaag tttcaaaccc tggcaaattc
      301 gattggaaca taaagattta gaaaccaaat tccttcattc ttttttcacc acagaggaca
      361 cagaggaaca cagagggaaa tcaatcgccc ttgcctctgt ttcctctgtg gtgtcgctgg
      421 gttttctgca aaccgacttg caagctggtg acacctgggc gaaactgcgg gcgttcaact
      481 cttcaaacgt tcgttcaatc aactgtacat tatgagttca aatccattac taccttcatc
      541 agccaattca atcacagaag tcgccacgct cggcggtggc tgtttctggt gcctcgaagc
      601 cgttttcgat caacttcaag gcgtgcttca cgttgagtcg ggatattcgg gcggaaccgt
      661 tgaaaatccg acctatatgc aggtgtgcgc tgaaattact ggccacgctg aggttgttca
      721 ggtcaccttt gatccggctg tcatttcatt tcaggaaatc ctgcagatct tttttgtcat
      781 tcacgaccca accacattca accggcaggg cgctgacatc gggactcaat accggtcagc
      841 tattttctat cactctgcag agcaaaaaca gattgctgaa acgatgattc aggagttaaa
      901 tgcggttggg gtttatgacc gaccgattgt cactgaagtg accgcattta cccggttttt
      961 tgaagctgag ccgtaccatc aggaatactt tgtccgcaac ccggctcaag gctattgcca
     1021 gtttgtggtg tcgcccaaag tggccaaatt ccgcaaactg tttgccgaaa aactgaagac
     1081 ctgaatgcct ccttcggcgg tgaaaaaaca gggccgtcca cgaaaccagg gttttgtgga
     1141 tggccctgtt tctgtttgca aaggagattt tttggctgaa tgatcaattt attgcttt
//
LOCUS       DAOQDO010002334         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152051_length_1198_cov_2.004374, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002334 DAOQDO010000000
VERSION     DAOQDO010002334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152051_length_1198_cov_2.004374"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1198)
                     /locus_tag="PKZ53_27040"
     CDS             complement(<1..>1198)
                     /locus_tag="PKZ53_27040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44170.1"
                     /translation="PEESKQLSQMYSEWQNPNGERGNSTRGFTLEEQRILTLYGQGSI
                     DELSARTLLSRVLLQYYYQTEPANLNQGYLKKKALLAQYSEFVRQQGRRILDEPRTND
                     KRPANTHINPNLAGGPDAFGYTFRDSNEAVNPPAFQFIDISATGTLLTRDAGSSADEG
                     AATLPLGGPGFSFYGNTINTVRVSTNGYISTALGDSGGDLSNDCPLPVIPSTGSGARF
                     YPLHDDLISANIRHQYFATSPRPADNGPNTGVNVISWINSDFFGGGTDAFTFQVLLYD
                     NGDIVYQYGPGNPQAGSGSTTGIQNDGATIGLTYACNTAATLADGLAVLFSTGGDGGG
                     GDCEPCTPDSNAFTDPSLEQEDTFWTSTSTNFGTGFCTIDNCGTGLGTGPRTGDVWWW
                     GGGTASA"
BASE COUNT          259 a          318 c          339 g          282 t
ORIGIN      
        1 cggctgacgc cgtaccgcca ccccaccacc agacatcacc ggtgcgtggg ccagtgccga
       61 gaccggtgcc gcagttatct attgtgcaga agccggtccc gaaattagtc gaggttgacg
      121 tccagaaggt atcttcctgc tccaggcttg gatcagtaaa ggcattgctg tctggggtgc
      181 agggctcgca atcgccacca ccgccatcgc caccagttga gaagagaacc gccagcccat
      241 cagccagcgt tgccgccgtg ttgcaggcat aggtcaaccc aatcgtggct ccatcatttt
      301 ggatcccggt ggttgagcct gaccctgctt gagggtttcc tggaccgtat tggtacacaa
      361 tgtccccatt gtcatagagc aggacctgga atgtgaatgc atcagtccca ccaccaaaaa
      421 agtcggagtt gatccagcta ataacgttga caccggtatt tggaccgttg tcggctgggc
      481 gtggagaggt cgcaaaatat tgatggcgga tattggcgct gatcaggtca tcgtgcagcg
      541 gataaaatcg tgcgccactg ccggtgctcg gtatgactgg caatggacag tcattgctga
      601 gatcgccgcc ggagtcaccc aacgccgtgc tgatataccc gttggttgaa acgcgcactg
      661 tgttaattgt gttaccataa aagctaaagc ctggtccacc cagcggaagg gtggcagcgc
      721 cttcatcagc actagaacca gcgtcgcgag tcaacagcgt tccagtcgct gaaatatcaa
      781 taaactggaa agccggcgga ttgacagctt cgttgctgtc acggaaggtg tacccaaacg
      841 catctggccc accggccaga tttgggttga tgtgggtatt cgccgggcgc ttgtcgttgg
      901 tccggggttc atcgaggatc cgccgtccct gctgtctgac aaactcagag tattgagcca
      961 gtaacgcttt ctttttgagg tagccttgat tcagattggc tggctcagtt tggtaatagt
     1021 attgaagcaa gacccgcgaa aggagggtcc tggctgagag ttcgtcaatg cttccctggc
     1081 catacagagt gagtattcgt tgctcttcaa gtgtaaagcc ccgcgtgctg ttgccacgct
     1141 caccgttcgg gttttgccac tccgaataca tttgggaaag ctgttttgat tcttcagg
//
LOCUS       DAOQDO010002335         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152179_length_1197_cov_3.705779, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002335 DAOQDO010000000
VERSION     DAOQDO010002335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152179_length_1197_cov_3.705779"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PKZ53_27045"
     CDS             complement(<1..959)
                     /locus_tag="PKZ53_27045"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44171.1"
                     /translation="MKPRIYLRQLAFSVLVLALLTTMKLPGCEALAEEPKAELKKLPP
                     VHWTRSRKIDVKHLVINVQFDWAKKQAFGTTAVTFAPFDSTSTIPLDAAFFTINSITT
                     QEGKVLKFEYDGGDQDDNLKITLDRVYQAGEETTVIINYRTNWVNLTDPNNLSGSTGK
                     GIRFFEPTSTDRTRPREIWSMGEPEGNRYWFPGFDSPNDFRTTELIATVDKKLTVISN
                     GTLIATRDNPDGTHTFHWKTDTPYANHLTSFVIGEYVDVQQNVDGIELHTFGYPNETE
                     AVAATVVRLPEMVKFYSEVTGVKYPYPRYSQVSVQDMPWGVGN"
     gene            complement(956..>1197)
                     /locus_tag="PKZ53_27050"
     CDS             complement(956..>1197)
                     /locus_tag="PKZ53_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44172.1"
                     /translation="SLKCLMDELTLLRKTTVQLFESFDEVALYRYTVINGNQMSALAY
                     GFAIPGHQKYHLTLIEEKYLPLLASSRVVIIPESL"
BASE COUNT          280 a          280 c          308 g          329 t
ORIGIN      
        1 ttatttccca caccccacgg catatcctga actgaaactt gtgagtagcg tgggtacgga
       61 tacttcactc cggttacttc agaatagaat ttgaccatct caggcaggcg aaccaccgtg
      121 gcggcaacag cttctgtttc attcggatag ccaaacgtgt gcagctcaat accgtcaaca
      181 ttttgctgga catccacata ctctccgatg acaaaagagg tcagatgatt ggcatatggg
      241 gtgtctgttt tccaatggaa cgtgtgtgta ccgtcggggt tgtctctggt tgcgatcagc
      301 gtgccgtttg aaataaccgt caacttttta tccaccgtcg ctatcaattc agtggtccga
      361 aaatcattgg gtgaatcaaa gccaggaaac cagtaacgat ttccttcagg ttcgcccatt
      421 gaccagattt ctcgcgggcg ggttcggtca gttgaggttg gttcaaagaa gcgaatccct
      481 ttgccggtgc tcccactgag gttgtttggg tcggtcagat tgacccagtt ggttcggtaa
      541 ttgatgatga cagtggtctc ttcgccagct tgatacaccc gatctagcgt gatcttcaga
      601 ttgtcatcct ggtcaccgcc atcatactca aatttcaaga ctttcccctc ttgagtcgta
      661 attgaattga tggtgaaaaa agccgcgtca agcggaatag tgctggttga atcaaatggg
      721 gcgaaggtaa ccgctgttgt tccaaaagcc tgctttttgg cccaatcaaa ctgcacattg
      781 atcaccaggt gtttgacgtc aatttttcgg gaccgtgtcc agtgaaccgg agggagtttt
      841 ttgagttcag cctttggttc ctcggcgagg gcttcgcatc cgggtaattt cattgtggtc
      901 agcagggcaa gcacgagaac ggaaaaagcc agttgacgca ggtagatcct tggtttcata
      961 gggactccgg tatgattaca actcttgagc tggcgaggag gggaaggtat ttctcctcga
     1021 tgagtgtcag gtgatatttt tgatggcccg gaattgcaaa tccatacgcc agggctgaca
     1081 tttgattgcc gttgatgacg gtatatcggt agagtgccac ctcatcaaag gactcaaaca
     1141 actgcacggt tgttttccgc agcagcgtta actcatccat caggcatttc aggctcc
//
LOCUS       DAOQDO010002336         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152337_length_1197_cov_1.633975, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002336 DAOQDO010000000
VERSION     DAOQDO010002336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152337_length_1197_cov_1.633975"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKZ53_27055"
     CDS             complement(<1..542)
                     /locus_tag="PKZ53_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNJ44173.1"
                     /translation="MAPQAFDSKQIALGITGGIAAYKACDVIRELYRQGAHQVIPMLS
                     PSAKAFITPLTLESLSRHRVIESDLETTDDGTPTHIALAQQCDGLLILPATANTLAKL
                     AHGLADNIITTTALTFTDKPVVVAPAMNTRMWFNPATQRNLSLLASYGHIDVIPPDAG
                     LLACGETGPGKLASLDLILL"
     gene            760..>1197
                     /locus_tag="PKZ53_27060"
     CDS             760..>1197
                     /locus_tag="PKZ53_27060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44174.1"
                     /translation="MGIQSLTATEASAASRFAGIRVDQNRTTGETSFSFLPPSSVPGL
                     QIVTHETITTPPMTSELLKGQQFTIPFNLYTAMTSNPTVNNGADSLVQQRFDNPTVAL
                     DQLPGLEHQAHGVQDDRGLRGTLFRLLTPLLPAQDQEPDQRRPN"
BASE COUNT          290 a          287 c          328 g          292 t
ORIGIN      
        1 taaagtaaga tcaagtctaa tgaagccagt ttccctgggc ccgtttcgcc acaggccagg
       61 aggcccgcat cggggggaat cacatcaata tggccgtagc tggccaggag ggataaatta
      121 cgctgggtgg ccgggttaaa ccacatgcga gtgttcatgg caggggcgac caccacgggt
      181 ttatcggtga aggtcagggc ggtggtggtg ataatattat cagccaatcc atgggccagt
      241 ttggccagag tgttagccgt cgctggcagg attaataagc catcacactg ctgagccaag
      301 gcgatatggg tgggggtacc gtcatcggtc gtctccaaat cagactcaat gactcggtgc
      361 cggctaaggc tttcgagggt taagggggtg ataaaggctt tggctgaagg cgaaagcatg
      421 ggaataactt gatgtgcccc ttgccgatac agttcgcgga tcacatcaca agctttataa
      481 gctgcaatac cgccggtaat acccagggca atttgttttg agtcaaacgc ttgaggggcc
      541 attggtggac ctgcttcctt gttgagctac agagtcttgg gattatgagg ccaaggttcg
      601 attaattata ggggaggcgg ggttcaatta cgacccagct ctccatggtg aaagggcgac
      661 cttgtgctgg acgaaccggt aatcgtcaat ttcgacccgg gtttcatttt tattcagtca
      721 ttgctgacac atgattcaac gaaaggaccg tttggcgtta tgggtattca aagtctgaca
      781 gctactgagg catctgcggc gtctcgtttt gccgggattc gtgtggatca aaaccggacc
      841 acgggagaaa cctcgttttc gtttttgcca ccatccagtg ttccagggct gcagattgtc
      901 actcatgaga ccattaccac cccgccgatg acgtctgaat tgctcaaagg ccaacagttt
      961 acgattccgt ttaacctata taccgcgatg accagtaatc caacagttaa caatggcgca
     1021 gatagcctgg ttcagcagcg atttgataac ccaactgtcg cgcttgatca acttcctgga
     1081 ttggaacatc aagcccatgg tgtccaagat gaccgagggt tgagaggtac actgtttcgg
     1141 cttttgacac cattacttcc tgcccaagat caagaacctg atcaacgccg ccccaat
//
LOCUS       DAOQDO010002337         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152410_length_1196_cov_3.585451, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002337 DAOQDO010000000
VERSION     DAOQDO010002337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152410_length_1196_cov_3.585451"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1196)
                     /locus_tag="PKZ53_27065"
     CDS             complement(<1..>1196)
                     /locus_tag="PKZ53_27065"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44175.1"
                     /translation="NLLSKKLEYYEFHIDLLMRMHQQDPSKGYNRQAFEFSDRTRARS
                     LAELLTEARVDIRQGVNRELLDRERNLREQFHARALELQQVTRQNTPKDQIETIEKNL
                     RSISEQYQQVQTEIRRQSPRYAQLTQPQGVTVDSLQKNILDADSALVEFSLGKEKSYV
                     WVLTTTGIESKSLPNQAEIEPLARTVYELLTTRQAKLTGTSLERLEQIAQADTEYWTA
                     AARLSEVLFQGLKLPATKKRLIIVPDGVLNYVPFAALPRWIKSERLPTKATSLPVPLI
                     QDFEIINLPSASVIGLLRTDFGERHQPSKHIAIFADPVFEPTDVRVTPVKPEPVPAVA
                     PVNLEQPVNRRMDLSMLKRLVFSRQEALGITSLVAPDQLSLALDFKANLPSVQSPALA
                     DYQIIH"
BASE COUNT          272 a          300 c          322 g          302 t
ORIGIN      
        1 gtgaatgatc tggtaatcgg caagcgctgg actctgaacg ctggggaggt ttgccttgaa
       61 gtcaagcgcc agggacaatt gatccggggc caccagcgag gtaattccca gcgcttcctg
      121 ccgcgaaaag accagccgct tgagcattga aaggtccatt cggcgattga ctggctgctc
      181 aaggttgacg ggtgccactg ccgggacagg ctcgggtttt acgggtgtca cccggacatc
      241 agtgggttca aaaaccgggt cggcaaagat cgcgatgtgc tttgacggtt ggtgccgctc
      301 accaaaatcg gtgcgcaaca aaccaatcac tgaggctgac ggtaagttga tgatctcaaa
      361 atcctgaatg agtggaacag gcaatgaggt tgcctttgtg ggcaaccgct cagatttgat
      421 ccatcgtgga agggccgcaa atggcacata attgagcacc ccatcgggaa caatgatgag
      481 ccgctttttg gtggcaggga gctttaagcc ctgaaacagc acctcgctca accgggcagc
      541 cgctgtccaa tactcagtat cagcctgcgc aatctgttca agccgttcaa gcgaagtccc
      601 ggtcagtttg gcctgacggg tggtcagcag ttcatagacc gtgcgggcca gcggttcaat
      661 ttctgcctga ttgggcaacg attttgactc aattccagtt gtggtcaaaa cccagacata
      721 actcttttct tttcctagtg agaattcgac caatgctgag tcggcatcaa gaatattttt
      781 ttggagcgaa tcgaccgtaa ccccttgagg ctgggttaac tgggcatagc gtgggctttg
      841 acgacgaatt tcggtttgca cctgttgata ctgctctgag attgagcgca ggtttttttc
      901 aatggtttca atctggtctt ttggggtgtt ttgcctcgta acctgctgca gttccagcgc
      961 tcgggcatgg aactgctcac gcaggttccg ttcacgatcc agcaattcac gattcacacc
     1021 ctgtcggatg tccactctgg cttcggtcag caattcagcc agtgaccggg cccgagtgcg
     1081 gtcgctaaac tcaaatgcct gccggttata tcctttcgat ggatcctgct ggtgcatccg
     1141 catcagcaaa tcaatgtgaa attcataata ttcaagtttt ttactcaaca aattac
//
LOCUS       DAOQDO010002338         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152413_length_1196_cov_3.427695, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002338 DAOQDO010000000
VERSION     DAOQDO010002338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152413_length_1196_cov_3.427695"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKZ53_27070"
     CDS             complement(<1..648)
                     /locus_tag="PKZ53_27070"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HNJ44176.1"
                     /translation="MVLPQQLKVEPGVSWLKMVFGVVFGLVISSQTVAAALQPVGDVT
                     TLLREKNGVVCQLSSRAKAEVRFVTPDVVRVRVLPPGFAEEDWSYAVTATPQPAPAVE
                     VSESGTTIKVTVAGGATVIIERKGFQLKVLDSSGKPIVVDDPVRPTAFDGQTGEVETS
                     KQRTPTELYYGFGEKALPMSRHDQFMVMWNTDTYGYPVGTDPIYQTIPFFIALSDA"
     gene            778..>1196
                     /locus_tag="PKZ53_27075"
     CDS             778..>1196
                     /locus_tag="PKZ53_27075"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44177.1"
                     /translation="MTAFLSHPSQVLAQSPTGSTTTGVASKPVTAKNNLKVFEVVWST
                     INKEYYDDDFNGVDWAKSRERFQPRAAAATTEKELYELLRDMVAELKDNHVSVKSPEV
                     AQLLKRRAFPNIGAEFRRIDQQVLVTEVKVPSSASEAG"
BASE COUNT          306 a          317 c          299 g          274 t
ORIGIN      
        1 ggcatctgac agcgcgataa aaaaaggaat cgtctggtaa atcggatcgg tgcccaccgg
       61 atagccatag gtgtcggtgt tccacatcac cataaactgg tcatggcgcg acatcggcaa
      121 cgctttttcc ccaaatccat aatacagctc ggttggcgtg cgctgtttgg acgtttcgac
      181 ctcacctgtc tggccgtcaa aggccgtagg gcgaaccgga tcatccacaa caatcggttt
      241 cccggatgag tccaaaacct tgagctgaaa gcctttgcgc tcaatgatga ctgtggcacc
      301 acccgccacc gtcactttga tcgttgtgcc ggattctgaa acctcaactg ccggagctgg
      361 ttgaggcgtg gctgtcaccg catatgacca atcttcttcg gcaaaccctg gaggcagcac
      421 gcgcacacgt accacgtcgg gtgtcacaaa gcggacttcg gcttttgccc gactggaaag
      481 ctggcacaca actccgtttt tctcgcgcag aagtgtggta acatcgccca ccggctgtaa
      541 cgccgctgct accgtctggg aagagatcac caaaccaaat acaacgccaa acaccatttt
      601 taaccaggag actcctggct caaccttgag ttgttgcggc aataccatag tcaattcctt
      661 gttgtgaaat gtgggggaaa tttcagacag cgtaccacaa aatcttttga tcagaatgga
      721 agatccggca tgagagcaaa atactttttg aacctggttc taattgtagt tgttggaatg
      781 acagcatttc tgagtcatcc gtcgcaggta ttagcccaat ctccgactgg ttcgaccaca
      841 accggagttg ccagtaaacc agttaccgca aaaaacaacc tcaaggtctt tgaggtggtc
      901 tggtcaacca tcaacaaaga atattatgac gatgatttta atggggttga ttgggccaaa
      961 tcccgtgaac gattccaacc acgcgccgct gcggctacca cggaaaaaga gctgtatgaa
     1021 ctcctgcgcg acatggtggc cgaactcaaa gacaatcatg tttcggtcaa atcacctgaa
     1081 gtggcccaac tgttaaagcg gcgggctttc cccaatattg gggctgaatt tcgacgcatt
     1141 gaccagcagg tcctggtgac cgaagtgaaa gttccgtcat cggcttcgga ggcggg
//
LOCUS       DAOQDO010002339         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_152785_length_1195_cov_2.370175, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002339 DAOQDO010000000
VERSION     DAOQDO010002339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152785_length_1195_cov_2.370175"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PKZ53_27080"
     CDS             <1..738
                     /locus_tag="PKZ53_27080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ44178.1"
                     /translation="KISVEAWTQAGVGVETGQTLVLISITDTGEGIPAESIPFLFDPY
                     WQANSTHTKPGVGLGLSIVKRIVAAHGGNISVRSQLGIGTCFTVVLPVVPFEPSLPVA
                     AIPVVASLPESSGLLSESPCILVADDEPVNLKVVSILLKRYGYVVDVVSDGCEAVTAA
                     MRKPYDLILMDCQMGKMGGIEAANLIRQEEGRTRHTPIIACTASDIFEMTPLIGSTFD
                     DVLAKPFTPEGLQHLLHKYGVQPVKSV"
     gene            complement(967..>1195)
                     /locus_tag="PKZ53_27085"
     CDS             complement(967..>1195)
                     /locus_tag="PKZ53_27085"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNJ44179.1"
                     /translation="VTASPAAPSGYKYQYSLANSTSLPSGLSLDANTGALTGTLVVGG
                     VRSFDNQRPDASAGDRRINVYVVPEGAQRPE"
BASE COUNT          258 a          306 c          318 g          313 t
ORIGIN      
        1 aaaatttcgg ttgaagcctg gacccaggct ggggttgggg ttgagactgg tcaaaccctg
       61 gtgctgatct ctattactga caccggtgaa ggcatcccgg cggaatcaat tccttttttg
      121 tttgacccct actggcaggc caattccaca cataccaaac caggcgtcgg tcttggatta
      181 tcaattgtca aacgcatcgt tgccgctcat ggggggaata tttcggtccg gagccagctt
      241 ggcatcggaa cctgtttcac ggtggtgtta cctgtggtcc cttttgagcc atccctgccg
      301 gttgctgcca ttccggttgt tgcttcattg ccagaatctt ctggtctgct ttctgagtct
      361 ccctgcatcc tggtggcaga tgacgagccg gtcaatctca aagttgtttc aattctttta
      421 aagcgatatg gatatgtggt tgatgtagtt tccgacggat gcgaagccgt caccgctgcc
      481 atgcgcaaac cctatgatct gattttgatg gattgccaga tgggaaaaat gggtggcatc
      541 gaggctgcca acctcatccg ccaggaagaa ggtcgcaccc ggcacactcc gatcattgcc
      601 tgtaccgcca gtgatatttt tgaaatgacc cctctgatcg gcagtacttt tgatgatgtg
      661 ctggccaagc cctttactcc agaggggctg caacacctgc tccataaata tggggtacaa
      721 cctgtgaaat cggtgtaata ccattttggg gtcagggtat cggttcagaa ggcagggaaa
      781 tccaattcct ccaatagttt agccttttgc taatacgata gtttcattcc aaaatggtgt
      841 aacaccagat tcccatcaat ttccaaaagt aaaagccact gccggaacga aatgtttcga
      901 cagtggcttt ttggttttga gtcaaggggg cacaccgttt gaaacggctc atcccttcac
      961 atcgggttat tcagggcgtt gcgcaccttc tggcaccaca tagacgttga tgcggcggtc
     1021 accggctgaa gcgtctggac gctggttgtc aaagctgcgc acgccgccca caaccaatgt
     1081 gcccgtgagc gcgccggtgt tagcatcgag cgacaagccg ctgggcagac tcgtgctatt
     1141 cgccagcgag tattgatact tgtatccgct gggggctgcc ggactggcgg tgacg
//
LOCUS       DAOQDO010002340         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_153235_length_1193_cov_3.962214, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002340 DAOQDO010000000
VERSION     DAOQDO010002340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153235_length_1193_cov_3.962214"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PKZ53_27090"
     CDS             <1..1060
                     /locus_tag="PKZ53_27090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ44180.1"
                     /translation="GVVLDMHFLKEEYLPKAIQKFLPKFFPDDTQKNVVVNLRQSCGE
                     ILLSTDVTKAREQEDYISLPMPFVFTDLSLGIRDCYLSPEQWAHSYFWTSLTLSGLMT
                     LVLMGGIWFTLRAAEREMKLCEMKTDFVSNVSHELRTPLSSIRVFAEFMKLGRVKDFE
                     KICEYGGYIENESQRLTQLINNILDFSKIESGRKMYRYEKANLADTINDTLKIVDLRL
                     KQSGFDVKVAMPEDPLPPTVIDRDAIAQALLNLLDNAVKYSGEAREIVVRLETEEGHL
                     AVSVTDQGIGIPLDDQEKIFEKFYRVCTGCVHDVKGSGLGLSIVKHIVDAHHGRITVH
                     SKPSQGTTFTIHLPVAES"
BASE COUNT          303 a          266 c          303 g          321 t
ORIGIN      
        1 tggggtggtt ctggatatgc actttctgaa ggaagagtac cttcccaaag ccatccagaa
       61 gttccttcca aagttttttc cggacgacac acagaaaaat gtagtggtga atctgcgcca
      121 atcctgcggt gaaattctgt tgtcaactga tgtgaccaaa gcccgcgagc aggaagatta
      181 tatttcatta ccgatgccct ttgtgtttac tgaccttagt ttgggaattc gtgattgtta
      241 tttatcgcct gaacagtggg cccactccta tttctggaca agcctgacct tgtcggggct
      301 gatgacgctg gttttgatgg gtggtatctg gtttacgtta cgtgctgccg agcgggagat
      361 gaaactgtgc gaaatgaaaa ccgattttgt ttcaaacgtt tcacacgagc tgcgcacgcc
      421 gctttcctca atccgggtat ttgctgaatt tatgaaactg ggacgggtca aggatttcga
      481 gaaaatctgt gaatatggcg gatatatcga aaatgaaagc cagcgattga ctcagcttat
      541 caataacatc cttgatttct caaagattga gtctggccga aaaatgtatc ggtacgaaaa
      601 ggccaatctg gctgacacca ttaatgacac gctgaagatt gttgacttgc ggctcaagca
      661 aagcggcttt gatgtcaaag ttgctatgcc tgaggaccca ttacctccca cggtcattga
      721 ccgggatgca attgcccagg cgttgttaaa cctgctggat aatgccgtta aatattctgg
      781 cgaggccagg gagattgtgg ttcggctgga aaccgaagaa gggcatctgg cggtttccgt
      841 caccgatcag gggattggaa tcccgcttga tgatcaggaa aaaatctttg aaaaatttta
      901 tcgtgtctgc accgggtgtg tgcacgatgt caaagggagc ggactcggac tgtcaattgt
      961 caaacatatc gttgacgctc atcacgggcg cattactgtc cacagcaagc ccagccaggg
     1021 aacaacgttt acgatccatc ttccggttgc cgaaagctag gtgaatgaca ttgtgaaaga
     1081 gcaaccagag cgaatggtga aagagtgagc cttgcggtcc aatccattga tatcaacact
     1141 gaaactctgc gctgctgatc tttctccgtc gttctttcac cctttcgctc ttt
//
LOCUS       DAOQDO010002341         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_153482_length_1192_cov_3.861917, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002341 DAOQDO010000000
VERSION     DAOQDO010002341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153482_length_1192_cov_3.861917"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="murC"
                     /locus_tag="PKZ53_27095"
     CDS             complement(<1..927)
                     /gene="murC"
                     /locus_tag="PKZ53_27095"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNJ44181.1"
                     /translation="MFKRIRHIHFVGIGGIGMSGIAEVLLNLGYKVSGSDLKLTGVTQ
                     HLAERGAIVYEGHAAQSVTGADVVVISAAVKPNNPEVVEALHQQIPVIPRAEMLAELM
                     RLKYGIAIAGAHGKTSTTSMIAHILHRADYDPTVVIGGKLGTLGSNARLGHGEFIVVE
                     ADESDGSFLKLSPTIAVVTNIDREHMDYYKSLDEICEYFAAFVNRVPFYGSVVVCLDD
                     SNVQSIIPKIKRRTLTYGFSAQADVTAWNVKLTELFGSSFTVRFRGQELGRIELQVPG
                     THSVYNALAAICTCLDLEVPFDKIASALSNFRG"
     gene            complement(1004..>1192)
                     /locus_tag="PKZ53_27100"
     CDS             complement(1004..>1192)
                     /locus_tag="PKZ53_27100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNJ44182.1"
                     /translation="SELNGERLAKEILSLIDKPERITAMEAASRNLARPNAAGQVVDL
                     ALRVASNSSFSELVVLNP"
BASE COUNT          284 a          321 c          300 g          287 t
ORIGIN      
        1 gccacggaaa ttactcaggg cgctggcaat tttgtcaaac gggacttcca gatcaaggca
       61 ggtgcaaatc gctgctaaag cattgtaaac actgtgtgtt ccaggtactt gaagctcaat
      121 tctccccagc tcctggcccc ggaagcgaac cgtaaaagat gacccgaaga gttccgtgag
      181 tttcacattc caggcggtga cgtcagcctg tgccgaaaac ccgtaggtca gtgtccggcg
      241 cttgattttg gggatgatag attgcacgtt tgaatcatcc aggcacacca ccacgctgcc
      301 ataaaaaggg acacggttga caaaggcggc aaagtattca cagatttcgt ccaggctctt
      361 gtaatagtcc atgtgctcgc ggtcgatatt ggttaccacc gcaatcgtgg gcgaaagctt
      421 caaaaatgag ccgtcgcttt cgtcagcttc gaccacaata aactcgccat gcccaagtcg
      481 agcattgctg ccgagtgtgc cgagttttcc gccaatcacc acggtcgggt catagtctgc
      541 ccggtgcaga atatgtgcaa tcattgacgt ggttgaggtt ttcccgtggg cgccagcgat
      601 agcaatgcca tatttgaggc gcatcaattc agccagcatc tcagcccgtg gaatcaccgg
      661 aatttgctgg tgcagggctt caaccacttc cgggttattg ggtttgacgg ctgctgaaat
      721 aaccaccacg tcagcaccgg tcacgctttg tgctgcgtga ccttcataga caatggcccc
      781 tcgttcggcc agatgttggg tgaccccagt cagtttcaaa tcagagccgg atactttata
      841 gccaaggttg agcagcactt cagcgatgcc gctcatgccg attcccccaa tacctacaaa
      901 gtgaatgtgt cgaattcgtt taaacatatc cttggaaaaa gtaccttgtc cttagtggct
      961 ggtggctggt ctcgaaaacc ctgacccctg accccttgaa ctcctacgga ttcaagacta
     1021 ccagttctga aaaagatgaa tttgatgcca ctcgtaaggc gagatcaacc acctgaccag
     1081 ccgcatttgg acgggcgaga ttccggctgg ctgcttccat ggccgtaatg cgctcgggtt
     1141 tatcaatcag cgacagaatt tctttggcca gccgttcgcc gttcaattct ga
//
LOCUS       DAOQDO010002342         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_153743_length_1191_cov_3.450704, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002342 DAOQDO010000000
VERSION     DAOQDO010002342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153743_length_1191_cov_3.450704"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..515
                     /locus_tag="PKZ53_27105"
     CDS             93..515
                     /locus_tag="PKZ53_27105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44183.1"
                     /translation="MSDLQPGIRNPGTNSIFLCSLHPQAAPRLCRFNPDQNIWEKGEW
                     PRLCTSVSGVLNPIEAGPGQSIKLKAGWRYSLDDENTPTVFVTDKEKNRPLNGKYKLL
                     LQYSEEPWSYINTPKRLFTVESPVFEVKIENTSFAHEN"
     gene            539..1063
                     /locus_tag="PKZ53_27110"
     CDS             539..1063
                     /locus_tag="PKZ53_27110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44184.1"
                     /translation="MNYYKYTDEESPIGAGTRYVEVSNRWAIREVMVDGELFLGSSIC
                     YPHWGLMLTDKAIDYDGIEEITKITKEEFDAVWTAHLIHNAGRWAIRKLAYPVGTHIQ
                     GQVAVIFPQGVIVDLGDAGTLGVANYDQCLAASTAGGVWSGFRISAVVQGYDELNHWL
                     ILDNPQVHNDEGCD"
BASE COUNT          335 a          267 c          267 g          322 t
ORIGIN      
        1 tgttttgcgg agaatcacca atttctccat cagccaggct ctcaccgaaa cctgacctga
       61 ggtttgaaaa tctgcaggct cagtactcta atttgagtga cttacagcca ggcattcgta
      121 atcctggcac taactctatt tttctttgtt ccttgcaccc acaagcagca ccccgattgt
      181 gtcgatttaa cccagatcaa aatatttggg aaaaaggcga atggcccagg ctttgtacgt
      241 ctgtttctgg ggtccttaat ccaatcgaag ctggtccagg ccaaagcatc aaattaaaag
      301 caggatggag gtattctctg gatgatgaaa ataccccaac tgtttttgtt accgataagg
      361 aaaaaaatcg cccattaaat gggaaatata aactcttgct tcaatactct gaagaaccct
      421 ggagttatat caatacaccg aaacggctgt tcacagtgga atcaccagtc tttgaagtca
      481 aaatagaaaa tacaagcttt gcccacgaga attaagctac cttgatgctc aaatccctat
      541 gaactactac aaatacaccg acgaagagag tcccattggc gcaggcacac gctatgttga
      601 agtcagcaat cggtgggcaa ttcgtgaagt tatggtggac ggagagctgt ttttgggctc
      661 cagcatttgt tatccacatt ggggcttgat gctgacggat aaggcaattg actacgacgg
      721 gattgaggaa attaccaaaa tcaccaaaga agaatttgat gcggtttgga cggctcacct
      781 catccacaat gctgggcggt gggccattag gaagctggcc tatccggttg gaactcatat
      841 tcagggtcaa gtggcggtga tttttcccca gggtgttatc gttgacctgg gtgatgccgg
      901 tacactgggc gttgcaaact atgatcaatg tcttgctgct tcaacggcag gtggagtgtg
      961 gtcaggcttt cgaatcagcg ctgtcgtcca gggctatgat gaactcaatc actggctgat
     1021 actcgacaac cctcaggtcc acaatgacga gggctgtgat tgattgaact gaaatcaaag
     1081 gacttaaagg acttaaagga cttaaatcaa agaactaaaa cctgaagatc atctcaattc
     1141 ttcattctca attcttcatt cttcattctt cattcttcat tctcaattct t
//
LOCUS       DAOQDO010002343         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_153769_length_1191_cov_2.831866, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002343 DAOQDO010000000
VERSION     DAOQDO010002343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153769_length_1191_cov_2.831866"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>1191)
                     /locus_tag="PKZ53_27115"
     CDS             complement(1..>1191)
                     /locus_tag="PKZ53_27115"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNJ44185.1"
                     /translation="NKITGCLLSIFTAGCLFLTPSSAPVLAQSGKPVSPDPILSYLQT
                     HPETTAVASFTVTENGEIDAKDPAVFYNSDLRLPLASTKKVLLLAAYARAVSLGWLAS
                     DQAVLIADWEAYYLPGEDGGAHSEALTWLNIPQDNYGFALDPHQTVTLDQMMAVMIRF
                     SDNAAPDYLIEKLGDQFLADTVELAGMTHQDLPLTACGLGLSWESHESGPLTEARVQF
                     LAGLSQEAFRAEVFRLTERFKDPAWKLAEFEWRLAGNSTFTPAIEIKAIDAVAEHGTV
                     RDYATLLSRVLSGRFVSPIVCQLIRKHLEWPMEIPEIRQGFAALGEKGGSFGAGVLTD
                     TLYCIPRTGDFGNKTRITVIFLHKLSRQEFKEFYLDGKMFGFELQLAVDRKFAKKARK
                     GLKP"
BASE COUNT          303 a          306 c          297 g          285 t
ORIGIN      
        1 ttatggtttc aagcctttcc gggctttttt ggcaaatttg cgatcaacgg ccagttgaag
       61 ttcaaatcca aacatttttc catccaaata aaattccttg aattcctggc gagagagttt
      121 gtgcaaaaag atcacggtga ttcgggtctt gtttccaaaa tcgccagtgc gcggaatgca
      181 gtacagtgtg tcggtcaaaa caccggcacc aaaggatcct cctttttcac ccagcgccgc
      241 aaatccctga cgaatttctg gaatttccat tggccattcc agatgcttgc gaatcaactg
      301 acaaactatg ggtgaaacaa aacgcccgga aagtacccga cttaaaagag ttgcgtaatc
      361 tcggaccgtc ccgtgctcag ccacagcatc aatggctttg atttcaatag ccggagtaaa
      421 ggtgctgttc ccagccagtc gccattcaaa ttccgccagt ttccaggccg ggtccttaaa
      481 acgttccgtc aaacgaaaaa cttcagctcg aaaggcttcc tggcttagcc cagccaaaaa
      541 ctggactctg gcttctgtga gcgggcctga ttcgtggctt tcccagctca gacccaatcc
      601 gcaggcagtg agtggcaaat cctggtgagt cattccagcc agttcgacgg tgtctgccag
      661 aaactgatct ccaagttttt caattagata atcaggtgcg gcgttatcgc tgaaccggat
      721 catcaccgcc atcatctggt cgagtgtcac agtttggtgt ggatcgaggg caaagccata
      781 gttatcttgc gggatgttga gccaggttaa ggcttctgaa tgcgccccgc catcttcacc
      841 gggtaagtag tacgcttccc aatctgcaat cagaactgcc tgatcagaag cgagccatcc
      901 cagagaaacc gcccgggcat aggcggccag cagcagaact ttcttggtcg aagccagcgg
      961 caatcgtaga tcagagttat agaaaaccgc tggatccttc gcatcaatct cgccattttc
     1021 agtcacggta aaactggcaa ccgcggtggt ttccgggtgg gtttgcagat aagaaaggat
     1081 cgggtctggg ctcacgggtt tgccagattg cgccaggact ggggcacttg aaggggtaag
     1141 gaacagacac ccagcagtaa aaatcgagag cagacaacct gttattttgt t
//
LOCUS       DAOQDO010002344         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_154033_length_1190_cov_2.758590, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002344 DAOQDO010000000
VERSION     DAOQDO010002344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154033_length_1190_cov_2.758590"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1190)
                     /locus_tag="PKZ53_27120"
     CDS             complement(<1..>1190)
                     /locus_tag="PKZ53_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44186.1"
                     /translation="PPFFNSGLTSNPPGPASLENLYELPGLSPDQEYVVLMEPTAGGS
                     FSSLTTFDVTSEFYNTGESGNPEIDQPLLQTSIRPQAGRLMEGINFIVNAGSARELLS
                     FPRITVSPESGAVAIATIGIPDPAPVPGVSVALRSRNKQVFADQDVLIPAGKTSVEVQ
                     FPRFGPGVAGFEVTAQGYLPTTLVIGAASAQPELNPLPPNLPASRKLAVGEIKLSEEE
                     PPFQYQVRLLEPGQAAVSERKGSLLARLPEIRGPNQSQGRITQMAFSLDGQTLYVAHG
                     FAGTSVFLVQGENTPAVASTLNPERPWASPETRRQTQPIQVRSAIVWRLTDSNGDGVA
                     EAVDYFETGTADIQDLVVTSTATGESLILASNFSDARSFFNRPPGTVAPLKAAILAYE
                     DSDA"
BASE COUNT          285 a          321 c          321 g          263 t
ORIGIN      
        1 ggcatccgaa tcctcataag ccaggatggc ggcttttaac ggcgccaccg tcccaggtgg
       61 ccgattaaaa aagctgcggg catcagagaa gttactggcc aggatcaagc tttcaccagt
      121 tgccgtcgag gtcaccacca gatcctgaat atcagccgtg ccggtctcaa aataatcaac
      181 cgcctcagcc acgccgtcac catttgaatc ggtcaatcgc cacacaatcg cgctcctgac
      241 ctggatcggt tgggtttggc gcctggtttc aggtgatgcc caggggcgtt ctgggttgag
      301 ggtactggca acagcgggcg tattttcacc ttgaaccaga aaaactgaag ttcccgcaaa
      361 gccgtgggcc acatagaggg tctggccatc gagactaaaa gccatttggg tgatgcgccc
      421 ttgcgattga ttgggccctc tgatttcggg gagccgcgcc agaagactcc ctttgcgctc
      481 tgagacagcg gcctgaccag gttcaagcaa ccgcacctga tattgaaagg gaggttcttc
      541 ctctgacagt ttaatttcac caacagccag ttttcgagag gcgggcagat ttggcggaag
      601 tggatttaac tcaggttggg cactggcggc accaatcacc agtgtggtgg gaaggtaccc
      661 ttgagcggtc acttcaaacc ccgctacccc aggaccaaac cggggaaatt gaacctcaac
      721 cgaggtcttt ccggctggaa tgagaacatc ctgatcagca aaaacctgtt tattcctgga
      781 tctgagtgcc acactgaccc caggtactgg agctgggtct ggaattccaa tggtggcgat
      841 ggccacagcc ccactctcag gtgaaaccgt aatccgtggg aatgaaagca gttccctggc
      901 cgacccggcg ttgacaataa aattaatgcc ctccatcagc cggccagcct gtggtcgaat
      961 actggtttgc agcagcggtt gatcaatttc agggtttcct gattcacccg tgttataaaa
     1021 ctcactggtg acgtcaaatg tggttaatga actaaaggac ccgccggcag ttggttccat
     1081 taaaacgaca tattcctgat ccggactcaa cccaggcaat tcatacaggt tttccaggct
     1141 ggctggtccc ggtgggttac tggtcaaccc cgagttaaaa aaaggcggga
//
LOCUS       DAOQDO010002345         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_154565_length_1188_cov_2.489850, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002345 DAOQDO010000000
VERSION     DAOQDO010002345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154565_length_1188_cov_2.489850"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1188
                     /locus_tag="PKZ53_27125"
     CDS             <1..>1188
                     /locus_tag="PKZ53_27125"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44187.1"
                     /translation="TDQTLSLHLQVAPQSPDAMRAALTVVARRKGRALDALTTAIETL
                     RRQQTPEIQKLLDDYARLAGQISVLTLRGPETRKLEEHLAYLKLLNEEREKLENEISH
                     RSLEFQAQTTPITLENIQKQIPINAVLVEFAVYTPYDPQTDKPGTPRFVVYVLNHQGK
                     ILFADLGEAAPIEEAISAFRKVLSNPKTDLVKDIIPAAKPLNKLILKPVLALVGKTKH
                     LLISPDGDLHVIPFAALMDDKGKFLTEHYTLTYFTSGRDLLRLAVKIENQQPPLIVAD
                     PDYMDGRGPQMMGQTIGRLMRLMGTRVEGEQLKTILPDADLKTRSDATEQVVKQVSRP
                     GLVHIATHGYFLENAAQSARDPLSDHPSRNIVSLSSHFEKEREANPLLRSMLFFAGAN
                     QGG"
BASE COUNT          332 a          330 c          274 g          252 t
ORIGIN      
        1 acaccgatca aaccctatcg ctccacctcc aggtggcgcc tcagtcaccc gacgcaatgc
       61 gcgccgccct gacagttgtc gcacggcgca aaggtcgtgc ccttgatgcc ctgacgacgg
      121 cgattgaaac cctgcgccgg caacaaacgc ctgaaattca aaaactgctg gatgactatg
      181 cgcgcctggc tggtcaaatc tcagttctga ccttgcgcgg acctgaaacg agaaaacttg
      241 aagagcatct ggcatacctg aaattactca atgaagagcg cgaaaaactg gaaaatgaga
      301 tcagccacag aagcctggaa tttcaagctc aaaccactcc cattaccctg gaaaacattc
      361 aaaaacagat tccaatcaat gcggttttgg tcgagtttgc agtctatacc ccttatgatc
      421 cacagaccga caagcctgga acaccccggt ttgtggttta tgtattgaat caccagggaa
      481 agatcctttt tgcggattta ggcgaagccg ccccaattga agaagccata tcagcctttc
      541 gcaaagtgtt aagtaaccca aaaactgacc tggtaaaaga tatcatcccg gcagctaaac
      601 ccctgaacaa gctcatctta aagccagtac tcgccttagt cggaaaaaca aaacacctgc
      661 tgatttcacc agatggcgac ttacacgtca ttccatttgc ggcgttgatg gatgacaaag
      721 ggaaattttt aaccgagcac tataccttga cctatttcac cagtggacgt gacctgttac
      781 ggctggccgt gaagattgag aaccagcaac cgccactgat cgtcgctgat ccagattata
      841 tggatggcag ggggcctcaa atgatgggcc aaaccattgg tcggctcatg cggctcatgg
      901 gaactcgggt tgaaggtgaa caactcaaaa caatcttacc ggatgcagat ctcaagacgc
      961 ggtctgacgc cacagaacag gtagtcaaac aggtttcgcg gcccggcctg gtccacattg
     1021 ccacccacgg ctattttctg gaaaatgccg cccaatcagc cagagacccg ctttcagatc
     1081 acccatccag gaatattgtt agcctgtcca gccacttcga aaaagaacga gaggccaatc
     1141 ccctgctccg gtcgatgttg ttctttgcag gcgccaatca aggtggca
//
LOCUS       DAOQDO010002346         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_154757_length_1187_cov_5.023852, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002346 DAOQDO010000000
VERSION     DAOQDO010002346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154757_length_1187_cov_5.023852"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKZ53_27130"
     CDS             complement(<1..331)
                     /locus_tag="PKZ53_27130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ44188.1"
                     /translation="MSPARLIRSFLCNNWLFGTLVLGFVLLNSIPVQALDPHKAVTQY
                     RQTIWTKEAGLPNNAILALAQTGDGYLWLGTFQGLVRFDGVQFTVLNKTTVPEFKNNT
                     TWKLLVSS"
     gene            complement(628..>1187)
                     /locus_tag="PKZ53_27135"
     CDS             complement(628..>1187)
                     /locus_tag="PKZ53_27135"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396369.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNJ44189.1"
                     /translation="DIRKTDSLVQEMFPHNESLQRWIRLASEKIHFQGLPARICWLGY
                     GERAEFGVALNELVRRGQVEAPIVIGRDHLDCGSVASPYRETEAMKDGSDAIADWPIL
                     NALVNTASGASWVSFHHGGGVGIGYSLHAGQVTVVDGTDESAKRAERVLTNDPAMGVF
                     RHVDAGYEEADHTAETKGVKIPMKA"
BASE COUNT          293 a          310 c          310 g          274 t
ORIGIN      
        1 ccgaactgac cagcagtttc caggtcgtat tatttttgaa ttctggaacg gtggttttgt
       61 tcaagaccgt aaactgaact ccgtcaaatc tgaccaatcc ctgaaatgtc ccgagccaga
      121 ggtagccatc accggtttgc gccagggcca gaatggcatt gttcggaagt cccgcttctt
      181 tcgtccagat tgtttggcga tattgtgtaa cagctttgtg tggatccaat gcctgtactg
      241 gaatcgagtt gagtaaaacg aatcccagga caagtgtccc aaatagccag ttattacaca
      301 aaaagctacg gatcaatcga gcgggggaca taattcaaac ctcggcaaac cagatacagc
      361 acacccaggc tgttcactgg ggaaaacaga ctgaaaccaa agattttgca ggagttttcg
      421 gggcaagaaa gagatctgag atatgtggca ggttagaaat cagagatgcc tgcctcactg
      481 aaggccgccg ggattgtagc tgagtttggt tgagggcaaa agggaaggaa cccatcctgg
      541 taacaggtac tcagatgaca ggtgaccagg tgacaatcag gctattctgt caaaaggtca
      601 cctggtcagt tgtgtgagca agaatggcta ggctttcatt ggaattttca cgcctttggt
      661 ttcagcagtg tggtcagctt cttcatatcc ggcatcaaca tgccgaaaca cgcccatcgc
      721 cgggtcgttg gtcaacaccc gttcggcacg tttggctgat tcgtcagtgc catccaccac
      781 cgtgacctga ccggcgtgca ggctgtatcc aatgccgacg cctccgccgt gatggaacga
      841 aacccaactg gcacccgagg cggtgttgac cagggcattc aaaatcggcc agtcggcaat
      901 tgcgtcggat ccatctttca tcgcttcggt ttcacgatac ggagatgcca ccgaaccaca
      961 gtccaggtga tcgcgcccaa tcacaatcgg tgcttcgacc tgtccacggc gaaccagctc
     1021 gttgagcgcc acgccaaatt ccgcccgttc gccatacccg agccagcaaa tccgtgccgg
     1081 gagaccctgg aagtggattt tttcactcgc cagccgaatc cagcgttgca atgactcgtt
     1141 gtggggaaac atttcctgga cgaggctatc ggttttgcga atatctt
//
LOCUS       DAOQDO010002347         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_154821_length_1187_cov_2.623675, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002347 DAOQDO010000000
VERSION     DAOQDO010002347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154821_length_1187_cov_2.623675"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1187
                     /locus_tag="PKZ53_27140"
     CDS             <1..>1187
                     /locus_tag="PKZ53_27140"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNJ44190.1"
                     /translation="WIGTTIGFLRWNPGTNRAMFFSRPFSDQTNKEATVCYFFEDHAG
                     TLWLATKGAGICRFDRQHNSFAQITETEGLPHNNTYAIFEDRDRKFWMSSDAGIIQFD
                     PQANTFKTFGPTDGLQGKEFNRHAHCQTASGKIFFGGPNGLNSFEPVRVVTNPLPPPV
                     RITSVRIGEVIHILDAWALSPELKLDYRQNSLTFEFAALDFHAPDKNQFAYQLEGFHH
                     DWVNAGNTHTATFTNLPAGQYRFRVKGSNNDGVWNETGSFVTIRLGAPPWKTWWAYGL
                     YSLVVMGGLMVAFQYQRTSIVAHAKVREAKLQTELIEVRAKGLEKENLQRMYAEAEIR
                     RKNTELEDANLKLKELDRLKASFAAMLVHDLKAPLTVVRTSLDLLETDQIFSESSASG
                     FIG"
BASE COUNT          304 a          273 c          304 g          306 t
ORIGIN      
        1 tctggattgg gacaacgatt ggatttctcc gatggaatcc agggacaaac cgggccatgt
       61 tcttttcgag acccttttct gatcaaacaa acaaagaggc gacggtctgt tatttctttg
      121 aagatcacgc cgggacattg tggctggcca caaagggagc tggaatttgt cgttttgacc
      181 ggcagcacaa ctcatttgcc caaattactg aaactgaagg tcttccgcat aataatacgt
      241 atgccatctt tgaagatcgt gaccgaaagt tttggatgag cagtgatgcc gggattatcc
      301 agtttgaccc acaggcaaac acgtttaaaa cttttgggcc aacagatgga ctgcagggaa
      361 aagagtttaa ccggcatgcc cattgtcaga ccgcgagtgg gaagattttc tttggcggtc
      421 caaacggcct caacagtttt gaaccagttc gggtggtaac caatccattg ccgcctccgg
      481 tgaggattac gtctgtacga atcggcgaag tgattcacat tctggatgcc tgggcactct
      541 ctccggaact gaaacttgat tatcggcaaa attcattgac gtttgaattt gcggcacttg
      601 attttcatgc gccggataag aatcagtttg cctatcaact tgaagggttc catcacgatt
      661 gggtgaatgc tggcaacacc cataccgcca cgtttaccaa tcttcccgcc ggccagtatc
      721 gctttcgggt gaaaggttca aataatgatg gcgtttggaa tgaaaccgga agttttgtca
      781 ctattcgtct gggggcaccg ccctggaaaa cctggtgggc ctatgggctg tacagcctgg
      841 tggttatggg cgggctgatg gttgcttttc aataccagcg aacctcgatt gttgcccatg
      901 caaaagttcg ggaagccaaa ctgcaaacag agttaattga ggttcgggcc aaggggcttg
      961 aaaaagaaaa cctccagcga atgtatgccg aagccgaaat ccgccgcaaa aatactgagc
     1021 ttgaagacgc caatctcaaa ttgaaagagc ttgaccgctt aaaagccagc tttgctgcga
     1081 tgctggttca cgatctcaaa gctccattga ctgtggttcg gacttcgctt gatctgcttg
     1141 aaacagacca gattttttct gaatcttcgg cgagcggatt tatcggc
//
LOCUS       DAOQDO010002348         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_154830_length_1187_cov_2.595406, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002348 DAOQDO010000000
VERSION     DAOQDO010002348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154830_length_1187_cov_2.595406"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKZ53_27145"
     CDS             complement(<1..237)
                     /locus_tag="PKZ53_27145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44191.1"
                     /translation="MAQPSINPNDWVMTQQAFEALLRWLHPDRDLAGQAYEKARVRLM
                     KYFEAQGCPFFAEYTDEVFHRAARKLAEGAEIQVN"
     gene            complement(318..>1187)
                     /gene="nadE"
                     /locus_tag="PKZ53_27150"
     CDS             complement(318..>1187)
                     /gene="nadE"
                     /locus_tag="PKZ53_27150"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNJ44192.1"
                     /translation="FLEKISYVPALTPEMTVEQLSQTLLTCVYQATENSGETTRTAAR
                     NVAEAVGAEYFEFNINPLVQQYVGMVSESLGRPLTWATDDLALQNVQARVRAPGVWLL
                     ANVKRALLLTTSNRSEAAVGYATMDGDTCGGLSPIGGIDKAFLREWLRWMEATGPDGF
                     SAVPALSYVNNQQPTAELKPQEFGQTDEADLMPYPVLDAIEKAAIRDKRDPKDVYQLI
                     KHTFGRVYPAASLKNWVMKFFRLWSINQWKRERYAPSFHLDDENLDPKTWCRFPILSG
                     NFERELEELKSVE"
BASE COUNT          279 a          313 c          305 g          290 t
ORIGIN      
        1 gttcacctgg atttccgccc cttcagcgag tttacgggcg gcacgatgaa agacttcatc
       61 ggtgtattca gcaaagaatg ggcacccttg cgcttcaaaa tatttcatca accgcaccct
      121 ggctttttca tacgcctgac cagccagatc gcggtcaggg tggagccagc gcagcaaagc
      181 ctcaaaggct tgctgagtca tcacccagtc attgggattg attgatggtt gggccacgtg
      241 gtgcagttct cccattgaaa tcagccgcgt gaagtcaaaa tctgtcggaa attgaagttt
      301 caacgttgag atacacatta ttcaactgat tttaactctt caagctcacg ctcgaaattt
      361 ccggagagaa ttgggaatcg gcaccaggtt ttcggatcca ggttttcatc atccaggtga
      421 aatgacggag cgtaccgctc gcgtttccat tggttgatgc tccacaatcg gaaaaatttc
      481 atcacccagt ttttgagtga cgccgctgga tagacacggc caaaggtatg tttaatcagt
      541 tgatagacat cctttggatc gcgtttgtct cgaatggcgg ctttttcgat ggcatccagc
      601 accggatagg gcatcaggtc ggcttcgtca gtctgaccaa actcctgggg tttgagttca
      661 gcagtgggtt gctggttatt gacataggaa agtgctggaa cggctgaaaa cccatcaggc
      721 ccggttgctt ccatccacct taaccactca cgcaaaaagg ctttgtcaat ccccccaatc
      781 ggagaaagtc cgccacaggt atcgccatcc atggtggcat agccaacagc ggcttcactg
      841 cggttgcttg tcgtcagcaa gagcgcccgt ttgacattgg cgagcaacca gacacctggc
      901 gctcgaaccc gtgcctggac gttttgcaag gccagatcat ctgtcgccca cgtcagcggt
      961 cgccccagac tttccgagac catgccgaca tactgttgaa ccagcgggtt gatgttgaac
     1021 tcaaaatatt cagccccaac ggcttcagcc acgttgcggg cggcggtgcg agtcgtctca
     1081 ccgctgtttt cagtggcttg ataaacacag gtcagcaggg tctgggagag ttgctcaacc
     1141 gtcatctcag gcgtgagcgc aggaacgtat gaaatttttt ccaaaaa
//
LOCUS       DAOQDO010002349         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155059_length_1186_cov_2.916888, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002349 DAOQDO010000000
VERSION     DAOQDO010002349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155059_length_1186_cov_2.916888"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            769..>1186
                     /locus_tag="PKZ53_27155"
     CDS             769..>1186
                     /locus_tag="PKZ53_27155"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ44193.1"
                     /translation="MNNGLIRLCVLSILLLFATVAASAQATDGGINGKVVDESGAVIS
                     GASVKVTNMETGFSSSATADASGLFRINYLPVGKYSISAEASGFKTTSIDNVGIALNS
                     IIDVTIKMLPGEVSEVVTVSGGDVLVETSSSILGNTF"
BASE COUNT          299 a          289 c          275 g          323 t
ORIGIN      
        1 ctctgtgttc ctctgtgtcc tctgtggtga aaaaatgatg aagttattta attttcattc
       61 cttagatccc cgcagcctcc tcaaactggg acttcagttt cttgaagatc cggctgatca
      121 cagagcagat tcccatcggg gtttgggcta aatctttcaa ttccacgaac tatccaatgt
      181 ttggtctggc aaaccagaag atgaatcggg ctgtttgtgg ataaaccggc ggccagctca
      241 ataacccgcg agtggttcaa ctggcgttta aagtgatgtt ctaaacctat gtggtgagga
      301 gggtaaacca gcctggcctg accctggctc tttcttgccg ggccaggtca gcttttttgg
      361 gcagcgttag ttgatgaaaa accaaaaatt cagaaatgct cctgagcttc aagctgaagt
      421 tcaccccaac ttcaaaaata cgaagacttc ttctgactca aaccaatttc caattctcaa
      481 atggaaattc caaaaaagag atcgcctgcc tgccccacct tctgtccatt tcgtcacaca
      541 gaatcagcta ttcctcactt tttcattgtt gtccttagaa attagattaa gtttgcggtc
      601 caaagtacaa gccaattcag atactcagtt cactggcgtc ggaggatgta cccattaaga
      661 ggtgcagctt tgacgttcct gggggtgttt ttgtctctca aagccgttgt tgggctttaa
      721 cagccaaccc gcaccctcaa cacacaagaa aaacaatggg aggacccaat gaataacggg
      781 ttaattcggc tttgcgtact gtccattctg ttgctcttcg cgacggtcgc ggcttcggcc
      841 caggccaccg atggtggcat caacgggaaa gtcgttgatg aatctggggc ggtgattagc
      901 ggcgcatcgg tgaaggtcac caatatggaa acaggcttca gttcaagcgc cacagccgat
      961 gcttccggtc tgtttcgaat caattacctt ccagttggaa agtattcgat ttcagccgaa
     1021 gccagcggct tcaaaacaac atccattgat aatgttggga ttgcactcaa ttccattatt
     1081 gatgtcacca tcaaaatgct gcccggtgaa gtcagcgaag ttgtgactgt ttctggtggc
     1141 gatgtgctgg tcgaaaccag cagttcaatc ctcggcaaca cgtttg
//
LOCUS       DAOQDO010002350         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155070_length_1186_cov_2.741821, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002350 DAOQDO010000000
VERSION     DAOQDO010002350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155070_length_1186_cov_2.741821"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKZ53_27160"
     CDS             complement(<1..755)
                     /locus_tag="PKZ53_27160"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ44194.1"
                     /translation="MGDKDELLKKLLVTFKVEAAEHIQALSAHLFQLEKETSWDKKKN
                     LMELLLREAHTLKGAARSVNLLEIETLCHRLETVFTSLKAREHRLTPELTDLIHQGIE
                     HLSRLLATVDLTPSTTEKSKIVLFTRRLERALENDSTPLPPVDPLPDPLPVTAEKPTG
                     AESVRIPGSKLTSLLLQAEEMLALKLMADQRAVEIHGLLEQFSLWKKEWSKIHPEIQR
                     LRHLAQEKREPSPTEFTAAAFPDFLDWNQMYLKT"
     gene            complement(877..>1186)
                     /locus_tag="PKZ53_27165"
     CDS             complement(877..>1186)
                     /locus_tag="PKZ53_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ44195.1"
                     /translation="MTQAVMATEQGAKVVETGVKLSLDAAETIRLLSESMNESSQAAT
                     QIVASCQQQLIGMDQVATAMDSIRQATSQNMAGTKQAEQAAQTLNQLGQSLRSMIERT
                     "
BASE COUNT          256 a          288 c          302 g          340 t
ORIGIN      
        1 gttttaaggt acatctgatt ccagtccaga aaatctggga acgcagccgc agtaaactca
       61 gtcggggaag gctctctttt ctcttgagcc agatgcctta atctttgaat ttcgggatgg
      121 atctttgacc actctttttt ccagagtgag aattgttcca gcaggccatg aatttcaact
      181 gcgcgctggt cagccatcag tttcagcgcc agcatttctt cggcttgtaa aagcagggaa
      241 gttaattttg aacctggaat gcggacgctc tcggcaccag tcggtttttc agcggtgaca
      301 ggcagagggt caggcagtgg gtcaaccggc ggcaacggcg tcgaatcatt ttctagtgcc
      361 cgttccaatc gtcgggtaaa gagtacaatt tttgattttt cagttgtgga cggcgtcaaa
      421 tcaaccgtgg cgagtaaccg gctgaggtgc tcaattccct gatggattaa gtctgtcagt
      481 tctggggtca gacgatgttc ccgggctttt aagctggtga aaacggtctc cagtcggtgg
      541 cagagggtct cgatttccag tagattgacc gaccgggctg cccctttcaa ggtatgagcc
      601 tcacgtaaaa gcaattccat cagatttttt ttcttgtccc aactggtctc tttttccaac
      661 tgaaaaagat gtgctgaaag ggcttgaata tgttcggcag cctcgacttt aaatgtgacc
      721 agcagctttt tcagtaattc gtctttatca cccataagtt ttgtgtctgg ttggactgag
      781 gaattgtaaa aagatgtggg gcaacatcaa aactgagttc tttagcccgt cttctgccac
      841 ccgaagaaac cggcggtcta ttttccgcgt ggcgggctag gttcgttcaa tcattgaccg
      901 cagactctga ccgagctgat tcagcgtctg agcggcctgt tcggcctgtt ttgttccagc
      961 catgttttgt gaggtcgcct gtcggatact atccatcgcg gtggccacct gatccatgcc
     1021 aatcagttgt tgctggcagg aagcaacaat ctgcgttgcc gcctgagatg attcattcat
     1081 actttcgctc agtaaccgaa tggtttcggc agcatccagt gataatttca cacctgtttc
     1141 caccactttg gcaccctgtt cggtcgccat caccgcctgg gtcatg
//
LOCUS       DAOQDO010002351         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155106_length_1186_cov_2.286472, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002351 DAOQDO010000000
VERSION     DAOQDO010002351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155106_length_1186_cov_2.286472"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKZ53_27170"
     CDS             <1..634
                     /locus_tag="PKZ53_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44196.1"
                     /translation="FIGWVLPEVIPPDIQADARAEIRAIKKETLEACLNDFEKEEADL
                     FFKEIEARPRAKSLETLVELIAKSQRTKALESYTRWFWSVGGLRKSYGFLAGNLKGRR
                     NWRYAMSDDLLAALVNLAFIETPDGNLDAARLRPRLPLREFLQFLELRFGILVDRPPT
                     FLDTAQNRQAALKNLEALKRRLRMMGFFEALSDDFNAQYLRAPLAQEGTR"
     gene            631..>1186
                     /locus_tag="PKZ53_27175"
     CDS             631..>1186
                     /locus_tag="PKZ53_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44197.1"
                     /translation="MRFDPALLLELARTLAQELSQAARGHCLRVDFLNQEDAFELCHQ
                     LRQQLKSGDMSAYVLDSASVGHELVILPERAIEIRNRKELRLCLIIPSGVVDAAASSL
                     TNSFAAFDLTKFFKQTTETLVGKLPDDFKELYRQLKQTFRIALRPAPEHWADFVSALQ
                     QATSPAEAGCELWRLGLIPDAGPAI"
BASE COUNT          285 a          292 c          315 g          294 t
ORIGIN      
        1 gtttattggt tgggttttac cagaggtaat ccctccagat attcaagcgg atgcacgggc
       61 cgaaattcga gcaataaaaa aagaaaccct ggaagcctgt ctcaatgatt ttgaaaaaga
      121 agaagccgac ttgtttttta aggagattga ggctcgacca cgggctaagt ctctggaaac
      181 actggttgaa ctcattgcca agtcgcaacg aacaaaagct ctggaaagtt atacacgatg
      241 gttttggagt gtcggtgggt tgcgcaagtc atatggcttc ctggctggaa acctcaaagg
      301 ccgccgcaac tggcgctatg ccatgagtga cgacctgctg gcagccctgg tcaatctggc
      361 gtttattgaa acgccggatg gaaatttgga cgccgcccgg cttcgaccac gactcccgct
      421 tcgagaattt ttgcagtttt tagaacttcg gtttggaatc ctggttgatc ggccaccaac
      481 gtttctcgat actgcccaaa accggcaggc tgccctgaaa aatcttgaag cattgaaacg
      541 ccgattgcgc atgatgggct tttttgaagc cctgtctgat gattttaacg ctcaatattt
      601 acgggcaccg ctggctcagg agggaacccg atgagatttg acccggcttt gctgttagaa
      661 ctggcccgaa cactggccca ggaattgtcc caggcggcca gaggccactg cctgcgggtt
      721 gattttctca accaggaaga tgcctttgaa ctttgtcatc aactcagaca gcagttgaaa
      781 tctggggaca tgtcagccta tgtgctggat tcagccagtg tgggtcacga actggtgatt
      841 ttaccggaac gtgccattga gattcggaac cgaaaagaac ttcggctctg tctgattatt
      901 ccttcaggcg tggtagatgc ggcagccagt tcgctcacca attcgtttgc agcgtttgat
      961 ttaacaaaat ttttcaaaca aaccaccgag accctggttg ggaagctgcc agacgatttc
     1021 aaggaactct atcgccagct caagcagacg tttcgaattg ctttacgacc agcccctgag
     1081 cactgggcgg actttgtcag tgccctgcag caggcaacct caccggctga agccggctgt
     1141 gaactctggc ggttgggctt gattccggat gccggaccag cgattg
//
LOCUS       DAOQDO010002352         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155390_length_1185_cov_2.058407, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002352 DAOQDO010000000
VERSION     DAOQDO010002352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155390_length_1185_cov_2.058407"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKZ53_27180"
     CDS             <1..780
                     /locus_tag="PKZ53_27180"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ44198.1"
                     /translation="WVSALGIESNFTKDQMNYSRAFYFNFLPFYHFGFRTNYAVNDKF
                     NITHWLVNGANQSEDFNGFKSQALLLNFKPTSRVSWNVNYYTGLESRDTVPSLNPGVP
                     SLPTQPGLSTDVVNPVPRGRLHIFDTYATWNATDRLTLAGEFDYVISRSQTFSAPSRV
                     TGGALYARYQFTPALALAARAEYLSDRGGLFSGVTQALKETTLTGEYKFADGFLARAE
                     WRRDFSNQAFFLTAVPGVLKKEQNTATLGLVWWFGRKQGSW"
     gene            883..1065
                     /locus_tag="PKZ53_27185"
     CDS             883..1065
                     /locus_tag="PKZ53_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44199.1"
                     /translation="MKQFVTLAGLKTRLDVVWQDLQLLAAVTLARLIVARQLRAETFE
                     KNDFMVVSRAATRYIG"
BASE COUNT          280 a          282 c          324 g          299 t
ORIGIN      
        1 tgggtcagcg cgcttgggat cgagagtaat ttcaccaaag atcagatgaa ttattcacgg
       61 gcgttttact ttaactttct gccattttat cactttgggt ttcgcaccaa ttatgccgtc
      121 aatgacaaat tcaacatcac tcactggctg gtgaatggtg ccaatcaaag tgaagatttt
      181 aatggcttta aatcccaggc attgctgctc aattttaaac caacaagccg ggtttcgtgg
      241 aatgtgaatt actacaccgg gcttgaatca cgcgacaccg taccgtcgct taatccagga
      301 gtgccatcgc tgccaaccca gcctgggctt tcaacggatg ttgtgaatcc ggtaccacgt
      361 ggccgactcc acatcttcga cacctacgcg acctggaatg ccactgaccg gctcacgctg
      421 gcgggcgaat ttgactatgt gatttcacgc agtcagacct tttccgctcc atcacgtgtg
      481 acaggcggtg cactctatgc ccggtaccag tttacaccag cactggcact ggccgcgcga
      541 gcggaatatc tctcagaccg gggcggcctg ttttcaggtg tgacccaggc gctcaaggaa
      601 accaccttga ctggtgaata caaatttgcc gatggatttc tggcacgggc cgaatggcgg
      661 cgggattttt caaatcaagc gtttttcctg accgccgttc cgggagtcct gaaaaaagaa
      721 caaaacactg ccacgcttgg gcttgtctgg tggtttggac gcaagcaggg aagctggtaa
      781 gcccgctgaa gacagcgggt tgaagattca caagctcagg gctgaagaaa accagggctg
      841 aagcgctttg acgaattgaa aggcgtgaaa aaggaatttc ttatgaagca gtttgtaaca
      901 ctggctggtt tgaaaacacg actggatgtg gtttggcagg acctgcaatt gctggctgcc
      961 gtaactctgg ctcggctgat tgtggctcgc cagcttcgtg cggaaacctt tgaaaaaaat
     1021 gattttatgg ttgtgtcgcg ggcggctacc cgctacatcg ggtagcggag ggggacgtca
     1081 ccatggctga tgtaatgatg tgtttggcga caatcgcctt ttttgtaatg gctgtggcgt
     1141 atgtacgcgg ctgtaaacga ttgtaaggca ggatgatatg acttt
//
LOCUS       DAOQDO010002353         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155394_length_1185_cov_2.027434, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002353 DAOQDO010000000
VERSION     DAOQDO010002353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155394_length_1185_cov_2.027434"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1185
                     /locus_tag="PKZ53_27190"
     CDS             16..>1185
                     /locus_tag="PKZ53_27190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ44200.1"
                     /translation="MVTDFRKLYDVLATYYDDRVEYEKDIWDLKVLGKSHHRSAGSKS
                     LNLTQVIQPWLRESARRFFRYAIAVYSTGDCLAKLQALNRFSRFLATHYPQATSSKID
                     RNLLLEYLGYLLSLKLADETRAKAVINLRIFLEMCGREGWGDFIRERLIYDEEIPRQW
                     KKVPRFIPQPVQQQIRTHLNQMVNPWQRMVLILQECGMRISELLTLPLNCLVQDSEGD
                     YFIIYYQGKMYQEHRQWVSRTVAEEMVLQQEEVRKSGISTNYLFPNAKGESFHQSTFQ
                     THLNQFLQEVGLKDAAGHLCHITAHMFRHTVATNLVNAQVPLLSIKRHLGHRAITTTE
                     RYTHIYDETVKQELTRSRHLIAPATGELIERRTTMDDPFLQLTKKDLHAQALGHGW"
BASE COUNT          323 a          292 c          284 g          286 t
ORIGIN      
        1 aaacacgaga aacgagtggt gactgatttc cggaaattgt atgacgtcct ggccacctac
       61 tatgacgacc gggttgaata tgaaaaagac atctgggact tgaaagtgtt gggaaaatcc
      121 catcatcgat cagccggttc caaatcactc aacttgaccc aagtgattca accctggctg
      181 cgtgaatccg cccgacgttt tttccggtat gcgatagcag tctattcaac cggagactgt
      241 ctggcgaaac tccaggcatt gaatcgattc tcacgctttc tcgctactca ctatccccaa
      301 gcaaccagtt caaaaattga ccgaaacctg ttactggaat atttggggta tctcctcagt
      361 ttgaaattgg cggatgaaac tcgcgccaaa gcagttatta acctgaggat ttttttggaa
      421 atgtgtggtc gtgagggatg gggggatttt atccgggaac ggttgattta tgacgaagaa
      481 atcccacgtc agtggaagaa agtgccgcgc ttcattcctc aacccgtaca acagcagatc
      541 cggactcacc tcaaccaaat ggtcaatccc tggcaacgca tggtgttgat tctgcaagag
      601 tgcgggatgc gaatcagcga attactcacc ctgccattga attgtctggt tcaggattcc
      661 gaaggtgatt attttattat ctattatcag gggaaaatgt atcaggaaca ccgtcaatgg
      721 gtctcacgca cggtggccga ggagatggtg ctccaacaag aggaagtccg aaaatcaggc
      781 atcagcacca attatctctt tccaaacgcg aagggagagt ccttccatca atcaactttc
      841 caaacccact tgaatcagtt tttgcaagaa gttggcctga aggatgcggc tggtcacctg
      901 tgtcacatta ccgcccatat gttccgacat acagtggcaa cgaatctggt gaacgcacag
      961 gtgccgttgc tgtccattaa acgccatctt ggtcaccggg ccattaccac cacggaacgc
     1021 tatacccata tttatgatga aaccgtcaag caagaactca ctcgctcacg acatttgatt
     1081 gctccagcaa cgggggaact gattgagcgc cgcacaacca tggatgatcc gttcctgcaa
     1141 ttgacaaaaa aggatttgca cgcccaggca ttggggcacg ggtgg
//
LOCUS       DAOQDO010002354         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155548_length_1184_cov_2.922055, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002354 DAOQDO010000000
VERSION     DAOQDO010002354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155548_length_1184_cov_2.922055"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..724
                     /locus_tag="PKZ53_27195"
     CDS             206..724
                     /locus_tag="PKZ53_27195"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNJ44201.1"
                     /translation="MQVKVLTLRFCKLTETFNDTALQAFIKDKEVISIRDHFFEQLGI
                     PYLTLVVIYRLSLVADAVSPGSSEKVKPDESWKELLTEAEMPLFNTLRNWRNERAKRD
                     GIPPYIILTNRQLAVIVKARPESVAQLGEIEGIGTAKLEHYGKEIASLLSSRQPAAPP
                     APEKEVQPNEPE"
     gene            711..1052
                     /gene="avd"
                     /locus_tag="PKZ53_27200"
     CDS             711..1052
                     /gene="avd"
                     /locus_tag="PKZ53_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HNJ44202.1"
                     /translation="MNQNEPMIVFIQWSQFVEWLFTTTDKFPKKARFSFSQRIEGLAL
                     DVVEDLVEARYTKNRGPILKRANLRLEKLRILLRLSHNLQYLSHASYEFATRGVNETG
                     KLLGGWMKSSQ"
BASE COUNT          347 a          257 c          295 g          285 t
ORIGIN      
        1 aggcgaaccc cccttctgag ggagaggttg aggggaacgt gtaaggaggt gtccgcaaag
       61 cggacccaaa attttgaaaa caagtcaatt ataccattta ggattcaggg tatcgggttc
      121 aaggttcagt gaaatgcaat attttcaatg atttaacttc cccataatac gagtccttca
      181 ttgcaaaatg gtattacagg aaaatatgca agttaaagta ctgacactcc ggttttgtaa
      241 actgacagaa acgtttaacg acacggcatt acaagctttt atcaaagaca aagaagtcat
      301 ttcgatccgc gatcattttt ttgaacaact cggtatcccg tatttgactc tggtcgtgat
      361 atatcgcctt tcactggtag ctgatgccgt gtcaccaggt tcttctgaaa aggtcaaacc
      421 agatgaatcg tggaaagaac tgctgacaga ggcggaaatg ccgcttttta ataccctgcg
      481 gaactggcgg aacgaacggg caaaacgaga tggaatacca ccgtatatca ttctcaccaa
      541 tcggcaattg gcggtcattg tgaaggctcg acctgaatca gttgctcaat tgggtgaaat
      601 tgaaggtatt ggtacggcaa aattggaaca ctacggcaag gaaattgcat ctctcctatc
      661 aagtcgccaa cctgcggccc ctcccgcacc agaaaaggag gttcaaccca atgaaccaga
      721 atgagccaat gattgtgttt attcaatgga gtcaatttgt ggaatggctg tttaccacaa
      781 ccgacaagtt tccaaagaag gcgcggtttt cattttccca gcgcattgaa gggctggctt
      841 tagatgtggt tgaagatctt gtggaagcac gatacaccaa aaaccgaggc cccattttga
      901 aacgagccaa cttgcgactt gaaaagttga gaatcttgct gaggttaagt cacaacctgc
      961 agtatttgtc acatgcatcg tatgaattcg ccacgcgtgg cgtcaatgaa acaggaaaac
     1021 ttctgggcgg ctggatgaaa agcagtcaat agactcggcc ctgaagggcc aagcttgagt
     1081 cgtggaacca cgacgccatc ctgatggatg gccggtgtgg ggagagcgac tcaggcttcg
     1141 gttgaccaga cccctgtgca aatcacagga aacgattggg aaga
//
LOCUS       DAOQDO010002355         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_155849_length_1183_cov_2.570035, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002355 DAOQDO010000000
VERSION     DAOQDO010002355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155849_length_1183_cov_2.570035"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1183)
                     /locus_tag="PKZ53_27205"
     CDS             complement(<1..>1183)
                     /locus_tag="PKZ53_27205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ44203.1"
                     /translation="LFRYSPHQKVPEAQEIPLLLRLLKDDQKTVWFEGTNFSNLPAIP
                     ALEYANNRINMVVAGLTFSLETPLEYQFKLDGVDTNWLPWSRQSVKEYTNLWEGTYQL
                     RVRARNIYGQMSRETLFSFRVFPPWYRTWWAYGLYIAGLAGLGYGGINWRLKILRRRN
                     EELEQLVALRTSELKDKVQELAQKNEELVASQRQADRIFTAFTEALPGTVLDGKYRLD
                     IKIGAGGVGAVFRATQIALDRKVAIKVFRPSPGHDSPKEVERFRREGISSCRVNHPNA
                     VSIVDSGISSDGIAYLVMELLQGHTLAKELKLRRRLSLKRCAAILNPLCDALSAAHQA
                     GIIHRDIKPENVFLHNTKAGQIVKILDFGIAKLVGEGNSSLEEDLTATGGIIGTPAYM
                     AP"
BASE COUNT          270 a          316 c          309 g          288 t
ORIGIN      
        1 ggggccatgt aggcaggtgt gccgatgatg ccgcctgtgg ccgtcaggtc ttcttctaaa
       61 cttgagttgc cttcgccgac cagtttggca attccaaaat caagaatttt gacgatttga
      121 cccgcctttg tgttgtggag aaaaacattc tccggtttga tgtcgcggtg aataatcccc
      181 gcctgatgag ccgctgacag tgcatcgcac agcggattga gaattgccgc gcaccgtttc
      241 aaggaaagcc gccggcggag tttcaattct ttggccagcg tatgtccttg cagcaactcc
      301 atcaccagat aggcaatgcc gtctgaggaa atcccggaat caacgattga aaccgcattg
      361 ggatggttga cccgacagct tgaaatgcct tcgcggcgga agcgttcgac ttctttgggt
      421 gaatcatggc cagggctggg ccgaaatact ttgatggcaa cctttcggtc cagcgctatt
      481 tgggttgctc gaaaaacagc gccaaccccg ccggctccaa tcttgatatc cagccgatat
      541 ttcccatcca gaaccgttcc agggagtgct tcggtaaagg ccgtgaaaat ccgatctgcc
      601 tgccgctggg aagctaccag ttcttcgttc ttctgagcca gttcctgaac cttgtctttt
      661 aattctgagg tgcggagggc caccagttgt tcgagttctt cattccgccg ccgtaaaatc
      721 ttcaagcgcc agttgatgcc gccataccct aaccctgcca gccccgcaat atagagtcca
      781 tacgcccacc aggtccggta ccagggcgga aaaaccctaa acgaaaagag cgtttcccga
      841 ctcatctggc catagatatt gcgggcccgg acccgaagtt gataggtgcc ttcccacaaa
      901 ttggtgtatt ccttaaccga ctgtctcgac cagggaagcc agttggtatc aaccccatca
      961 agtttgaact ggtattccag cggggtttca agtgaaaacg tcaagcctgc aacgaccatg
     1021 ttgattcggt tattggcata ctcaagggcg ggaatcgccg gcagattgga aaagtttgtt
     1081 ccttcaaacc agaccgtctt ttgatcatct ttgagcaggc gaagcaggag ggggatttcc
     1141 tgtgcctcgg gtactttctg gtggggggaa taccgaaaca atc
//
LOCUS       DAOQDO010002356         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_156177_length_1182_cov_2.047028, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002356 DAOQDO010000000
VERSION     DAOQDO010002356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156177_length_1182_cov_2.047028"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PKZ53_27210"
     CDS             complement(<1..465)
                     /locus_tag="PKZ53_27210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44204.1"
                     /translation="MPRMGKLLIILWFIGICGGMGSSQFISQAQKMTKNPPKKVPKIW
                     DDKLLATWSLPLAGIGAKPHYVSEKEYYALPVDNLRTYPVYHPDFEPKGYREWLKQQG
                     AQLLIEPEKIQTEADWVQAGQRVFDELDVPETRSDDPVILAYISNREVLKRDG"
     gene            798..>1182
                     /locus_tag="PKZ53_27215"
     CDS             798..>1182
                     /locus_tag="PKZ53_27215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44205.1"
                     /translation="MKILLCLLLGFSGLFPITPGPQKSPPLPPIVVTTKKVKPTKVNH
                     QATKAKLLGTHKFSVGGVFGNYSSTATVTERKGILSLQAQQFFSKGLEGQAIIDGIIT
                     EVNAHSFKVNLSAINGRASGWDHPTH"
BASE COUNT          285 a          293 c          286 g          318 t
ORIGIN      
        1 accatcgcgt ttgaggactt cacgattgct gatataggcc aggatcaccg gatcatccga
       61 ccgtgtttct ggaacatcaa gttcgtcaaa cacccgttgc ccggcctgaa cccaatcagc
      121 ttcggtttga attttctctg gttcgatcaa aagctgtgct ccctgttgct tgagccattc
      181 gcggtagcct tttggttcaa agtcagggtg ataaaccggg tacgtccgca gattatcaac
      241 tggcagcgca tagtattctt tttcagaaac gtagtgcggt ttggctccaa tcccagccag
      301 cggaagcgac caggtcgcca gcaatttgtc atcccagatt ttgggcactt ttttcggcgg
      361 attctttgtc attttttgag cctggctgat gaactgggac gagcccatcc caccgcatat
      421 cccgataaac cacagaataa tcaacagttt tcccattcgt ggcatactga ttcctttgct
      481 tttcccagat cccaaaccga gagcaatcgg tttctcacac cagtgaacac acgtttcacc
      541 agacattgaa aaacttctgg aacctggcaa ttgattttta aatttgaata tggataaggt
      601 ttgattgaat cagcgcgtat tactggggaa gacagtgggt tggatttgga gtgctgcggc
      661 tcgacgcagc ttttcgttca cggcggatgt ggagtgctgc ggctcgacgc agcttttcgt
      721 tcacagttat cttcgattgc gtgtcagatc gaaatggcgg atgtgtacga gaatgtcagc
      781 ttttcaaagg agtgaccatg aaaattctct tgtgcctgtt gcttggattc tccggcctgt
      841 tcccgatcac gccgggacct caaaaatctc ctcccctacc accaatcgtt gtcacaacca
      901 aaaaggtcaa gccaacgaag gtcaatcatc aggcgaccaa agcgaagttg cttggaacgc
      961 acaaattttc ggttggaggt gtattcggaa attattccag taccgcgacc gtcactgaac
     1021 gaaaggggat tttgtcactc caggcgcagc agttcttttc gaaagggttg gagggccagg
     1081 ccattattga tggcatcatt acggaagtaa atgcgcactc ctttaaagtg aacctctcgg
     1141 ccataaatgg ccgagcttct gggtgggatc atccaactca ca
//
LOCUS       DAOQDO010002357         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_156621_length_1180_cov_2.616889, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002357 DAOQDO010000000
VERSION     DAOQDO010002357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156621_length_1180_cov_2.616889"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKZ53_27220"
     CDS             <1..182
                     /locus_tag="PKZ53_27220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44206.1"
                     /translation="LTVRTYLVAEDYSLDEFMAMMKTRAQKEEEVRHFRTTFLIDSRN
                     LPPPEEEPEDEQQSE"
     gene            complement(183..>1180)
                     /locus_tag="PKZ53_27225"
     CDS             complement(183..>1180)
                     /locus_tag="PKZ53_27225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44207.1"
                     /translation="IKGPEPQIFTTTITRNSAQVQAGEVSFQTVGGVVVSPNKIKVRF
                     GPNQAQVQVKVVVTFLQPLDPKQTQIDVTWTDVSAVAPPVPKKAAFPPVETKSVPSPL
                     QLARIKVIPVSVALPAKLRVGYVRSFEFTLPQTLKLLGVEHRELQDSDWKPGGLKENF
                     DTIVLDNRAYLAAPDLASHQVQLMEFVEQGGHLVVFYHKTGEFKSEWAALPLKIGDRR
                     VAEETAPITFLLPEHPLLNWPNKLSQTDFDNWVQERGLYFPSEWDRGYQALLASHDTG
                     EEALPGGYLVAQRGKGSYVYTSYAWYRQLRVYHQGTFRIFANMIAYPVRPTNQSE"
BASE COUNT          287 a          318 c          296 g          279 t
ORIGIN      
        1 cgttgactgt ccggacctat ctggtggctg aagattatag cctcgacgaa tttatggcca
       61 tgatgaaaac cagggctcaa aaagaggaag aagtccgcca cttccgcacg acctttctga
      121 ttgattcccg caaccttccc ccacccgagg aagaacctga agacgagcaa cagagcgagt
      181 agctactcgc tttggttggt gggtcggacc ggataggcaa tcatattggc aaaaatccgg
      241 aatgtcccct ggtgatagac ccgtaactga cgataccagg cataactcgt atagacataa
      301 ctcccctttc cccgctgagc aaccagatag ccacccggca gtgcttcttc accagtatcg
      361 tgcgacgcca gaagcgcctg atagccacga tcccactcag atggaaaata cagaccgcgt
      421 tcctgaaccc agttgtcaaa atcggtctgg ctgagtttgt ttggccagtt cagaagtgga
      481 tgttctggaa gcaaaaatgt tatcggtgcg gtttcttcag ccacccgacg atcaccaatc
      541 ttgagcggca atgcggccca ttcggatttg aactcaccgg ttttatggta gaaaaccacc
      601 agatggccgc cttgttcgac aaattccatc agttgtacct ggtgactggc tagatccggt
      661 gccgccagat acgcccgatt gtcgagcaca atcgtgtcaa agttttcttt gagcccccct
      721 ggcttccagt cagagtcttg caactcacgg tgctccacac ccagtaattt cagcgtttgt
      781 ggcagggtaa attcaaagct gcgcacatac ccaacccgga gctttgccgg aagagcaact
      841 gaaactggaa tcactttaat tctggccagt tgcagcggtg agggaacact tttggtctca
      901 actggaggaa aggcagcttt tttggggacc ggcggcgcta cagctgaaac atccgtccag
      961 gtcacatcaa tctgggtttg tttcggatcc aatggctgca aaaaggtcac caccacttta
     1021 acctggacct gcgcctgatt tggcccaaat ctcaccttga ttttatttgg tgaaaccacg
     1081 acgccaccca cggtttgaaa gctgacctct ccggcctgaa cctgagccga gttgcgcgtg
     1141 atggtggtgg taaagatttg cggttcgggg cctttgatat
//
LOCUS       DAOQDO010002358         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_156868_length_1179_cov_3.469751, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002358 DAOQDO010000000
VERSION     DAOQDO010002358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156868_length_1179_cov_3.469751"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKZ53_27230"
     CDS             complement(<1..580)
                     /locus_tag="PKZ53_27230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44208.1"
                     /translation="MQNGTLAGSSIQVNESAIRLMPFDTQGELQFSVTNSTGRPVQAT
                     IECELLDPEDAATRRATIQTILDPGAQIIPVNLSLGKLFKDKPESLYLHRVRYRVTPE
                     PKTASVVSGVVAVSEITPDCFQLRVATPERLTSPGAYKVLVMAFHPITGKPVSDVQLV
                     ATIKSANQSQPVTFSVQGATDISGTVRDEDGNP"
     gene            complement(1012..1161)
                     /locus_tag="PKZ53_27235"
     CDS             complement(1012..1161)
                     /locus_tag="PKZ53_27235"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNJ44209.1"
                     /translation="MKQRIETLPVARLEELGDALLDFQEVKDLEDWLAQPPQPTPAPE
                     TSTES"
BASE COUNT          299 a          291 c          294 g          295 t
ORIGIN      
        1 cggggttgcc atcctcatcg cggacggtac cgctgatgtc ggtggcaccc tgtacagaaa
       61 aggtaacggg ttgtgactga ttggcactct tgatggtggc aacgagctgc acgtcactca
      121 ctggttttcc agtgatcggg tgaaatgcca taaccagtac tttgtaggct ccaggagatg
      181 tgagtctttc tggcgttgcc acccgcaact gaaaacaatc tggcgtgatt tctgaaactg
      241 caacgacacc gcttacgacg ctcgctgttt ttggctccgg cgtaacccga taccgaaccc
      301 gatgcaggta cagggattcg ggcttatctt tgaagagttt tcccaatgac agattgaccg
      361 ggataatttg tgccccagga tcaagtatcg tctggattgt ggcacgcctc gtggccgcat
      421 cctccgggtc aagcaattca cattcaatag ttgcctgcac tggccgtccg gttgaattgg
      481 ttacactgaa ttgaagttca ccctgggtat caaaaggcat cagacggatg gccgattcat
      541 tgacctggat tgaactaccg gccagggttc cattttgcaa taaactcctc gcttcaccag
      601 caagccagtt cgttgaaaag attggatttc cctgaaccct gaaccctgaa ccctgaaccc
      661 ttgatggtat aactccaaca ttgacagcca gaagcagaca cgacaccact ccaaacagaa
      721 cacgccaaat cgccaaacgc atatggattg ttctcttttc ttttcaggaa ttgaaatttt
      781 tggaatcaat tgtcaaggct gaaggaaacg cctgacagat ggtgaatgcc tgtttgaact
      841 gtgaaatttc aggagttatg gctgatactc aaaaactggg caacttacct ggcagaataa
      901 ggatttgaag ctcagcaggt cgtggtgcaa tagcggttag gccaggttca gggccacaac
      961 caaattgcaa gtgatgaagt cttgcggaag gcgcaaatac gccaccattt tctatgattc
     1021 agttgaagtt tctggcgcag gtgtcggctg aggaggctga gccaaccagt cttccaggtc
     1081 tttgacctct tgaaaatcaa gcaacgcatc cccaagttct tccagtcggg cgactggtaa
     1141 cgtttcaatt cgttgtttca ggtcagccga aaacagacc
//
LOCUS       DAOQDO010002359         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_156977_length_1179_cov_2.144128, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002359 DAOQDO010000000
VERSION     DAOQDO010002359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156977_length_1179_cov_2.144128"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>1179)
                     /locus_tag="PKZ53_27240"
     CDS             complement(78..>1179)
                     /locus_tag="PKZ53_27240"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNJ44210.1"
                     /translation="MSSALSLRIFSPQPCVFSPTSSAPSLRVFNPNFMTPRLQTFLDH
                     FFSDKSPHIDRLQGDASTRAYFRVSLPEQTYIAAVYPDSFSLAEFPYLVVTQLFEQFA
                     VPVPRIHSISEVEGIILQEDLGDLRFQDVLSDLTPARFNELYREAITLLVEIQKTTDQ
                     AVTQNLLPARLAFDEEKLFWELNFFFEHYFESYRQQRPKAEYETLILGELFALARRLA
                     GVPRVLCHRDYHCRNLMYTANRLVVIDHQDARMGPATYDLASLLGDPYARLDQHLQDE
                     LKTFFWEQHTAAFGNRIYPSRDVFEAEYRLMMIQRMLKAVGTFSFQAGVRKNSTYIEY
                     ITPAFQASLKALEQVADLPFTQQAVEKALLQE"
BASE COUNT          302 a          266 c          323 g          288 t
ORIGIN      
        1 tcatcattgg gaggatgttg ctcgaccata attgaatccc agtcagtacc gcctgccgga
       61 gcggtggaag tgattcttca ttcttggagg agggcttttt caaccgcttg ttgtgtaaac
      121 gggaggtcgg cgacctgttc cagtgctttt aatgacgcct gaaatgctgg cgtgatgtac
      181 tcgatatagg ttgaattctt tcgaactcca gcctgaaaac tgaatgtgcc gacagctttg
      241 agcatgcgtt gaatcatcat caaccgatat tccgcttcaa acacatcgcg tgacgggtag
      301 atccggttgc caaatgcggc ggtatgttgt tcccagaaaa aagttttcag ttcgtcctgg
      361 aggtgttgat ccagtcgagc atagggatcg cccagcaagg aggccagatc ataggttgcc
      421 ggtcccattc gggcatcctg atgatcaatc accaccagcc ggttagctgt gtacatcagg
      481 ttccggcagt ggtaatcacg atggcacagg acgcgtggga cacctgccag gcgacgggcc
      541 agggcaaaga gttcacctaa aatcaacgtt tcatattcgg cttttgggcg ttgttgacgg
      601 tagctctcaa aatagtgctc aaagaaaaag ttcagttccc aaaacaattt ttcttcgtca
      661 aaggccagtc ttgccgggag cagattttgg gtcaccgcct gatccgtggt tttttgaatt
      721 tcaaccagca acgtgatggc ttcgcgatag agttcgttga atctggccgg tgtcaggtcg
      781 cttaaaacat cctgaaatcg caaatcaccc aaatcttctt gcagaataat accttccacc
      841 tcactgatcg aatgaatccg tggtacgggg acggcaaact gttcaaagag ctgggtcacg
      901 acgagatagg ggaattcagc aagtgaaaag ctgtccgggt acaccgccgc aatataggtt
      961 tgctctggaa gcgaaactcg gaaataggcg cgggttgagg catcaccttg cagacgatca
     1021 atgtgtggtg atttgtcaga aaaaaaatga tccagaaatg tctgtaatcg aggggtcata
     1081 aaatttgggt tgaagactcg caagctcggg gctgaagacg tcgggctgaa gacacagggc
     1141 tgagggctga agattcgcaa gctcagggct gaagacatc
//
LOCUS       DAOQDO010002360         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_157164_length_1178_cov_2.694568, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002360 DAOQDO010000000
VERSION     DAOQDO010002360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157164_length_1178_cov_2.694568"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKZ53_27245"
     CDS             <1..654
                     /locus_tag="PKZ53_27245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ44211.1"
                     /translation="TGTTSLPEGGSVAPDHPVWVRFARGMAPMMAMPSQMLAKLIELP
                     SDRPSKVLDIAAGHGLYGIAFAQTYQQAQVTAVDWASVLEVARENAEKAGVLDRHHLL
                     PGSAFEVEFGTGYDIVLLTNFLHHFDIPTCEKLLEKIHASLADGGRVVTLEFVPNEDR
                     VTPPMAAMFSLTMLNGTPAGDAYTFAQYDEMFAKAGYSRNELHELPPTIQRVVVSFK"
     gene            926..>1178
                     /locus_tag="PKZ53_27250"
     CDS             926..>1178
                     /locus_tag="PKZ53_27250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44212.1"
                     /translation="MVCHEWFWPFATPTKETRVNVHMKIQLIPPTEKYRDSFLQGLGE
                     FRQEGLPWWVKGDIEIAEQDFAAFVAKKLADANRRTETFV"
BASE COUNT          278 a          291 c          326 g          283 t
ORIGIN      
        1 actggtacca cgtccttgcc ggagggcggg tcggtggccc cggatcatcc ggtctgggtc
       61 cggtttgccc gtggcatggc accaatgatg gcgatgccgt cacagatgct ggccaaattg
      121 atcgaattgc cgtctgatcg gccttccaaa gtactcgaca ttgccgccgg gcatggtttg
      181 tatgggattg cttttgctca gacttaccag caggcccagg ttaccgccgt tgactgggcc
      241 agtgtgctcg aagttgccag agaaaatgct gaaaaagctg gtgttctgga ccgccatcac
      301 ctgttgccag gcagtgcctt tgaggttgaa tttggaacag ggtacgacat tgtgctgctg
      361 accaatttcc tgcaccactt tgacattccg acctgtgaaa agctgctgga aaaaatccat
      421 gcttcactgg ctgacggagg ccgcgtcgtg acgcttgaat ttgtcccgaa tgaagaccgc
      481 gtgacgcctc cgatggcagc gatgttcagc ctgaccatgc tcaatggaac gcccgccgga
      541 gatgcttaca cgtttgccca gtatgacgaa atgttcgcca aagccggcta cagccgaaac
      601 gaacttcacg aactcccgcc gacgattcag cgggtggtag tgtcatttaa gtaaaaccat
      661 tgagggttca gggttcaggg ttgcggggaa aggcaatccc ttcaatagtt tgccgccctt
      721 ctattttcag gacctgattc cgaaaagggt acaagcccag acgttgatac aacaaaaact
      781 ggttgaactt ctgttgcgag ttcaaccggt ttgcttttta tgggcagaaa ataagcgggt
      841 ttaaggaacg atctcttgtc ttcttctttc acaggcaata actgggtggt agcctgagat
      901 aatttgaggc tactatacta acccaatggt atgccacgaa tggttctggc cattcgcgac
      961 gccgacgaaa gaaacgcggg tgaatgttca catgaagatc cagttaattc cgccgacaga
     1021 gaaatatcga gacagcttct tgcaggggct tggtgaattc cgccaggaag gtctcccatg
     1081 gtgggtcaaa ggcgatattg agattgccga acaggacttc gctgcgtttg tggctaagaa
     1141 gctggcggac gccaatcgaa gaaccgaaac ctttgttc
//
LOCUS       DAOQDO010002361         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_157198_length_1178_cov_2.337489, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002361 DAOQDO010000000
VERSION     DAOQDO010002361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157198_length_1178_cov_2.337489"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1119
                     /locus_tag="PKZ53_27255"
     CDS             130..1119
                     /locus_tag="PKZ53_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNJ44213.1"
                     /translation="MRYPKIIGTGSFVPEKVLTNADMSQMLGEDINEFVSQIVGISER
                     HICAEHESTADLATAAARNALAAANIKPEDLDLIILATDTPEYLSPSTSVVVQHRIGA
                     KNAGTFDVNCACAGFVTALDTACKFIVADEAYTNILVIGAYAMSKFLDFTEKKTCTIF
                     ADGAGAVVVQASTEGPGLLASKLVADGAFHDHMGIYAGGTHMPVTEEVLHEGRHTKVR
                     FAKKYPPEINLEGWPAIIRDVLAKAKLQLDDVDFFVFTQVNLSTIKIVMQTLELPWEK
                     THTIMGKWGYTGSACIPMCLDDAVQAGKIKPGMKIILCASGGGLNMACALVQW"
BASE COUNT          270 a          283 c          328 g          297 t
ORIGIN      
        1 atgccttttt catcgaacgg gctggcgagt tcaaccagac ggtacttgaa tttttaaaag
       61 ggaattaacg gattccacac ccacccgctg acgcaggtgg tactgactca tttcattctt
      121 tattccatta tgcgctatcc aaaaattatt gggacgggtt cctttgtccc tgaaaaagtc
      181 ctgaccaatg ccgatatgag ccagatgctt ggggaggaca tcaatgaatt tgtgtcccag
      241 attgtcggca tttccgaacg tcacatctgt gctgaacatg aaagcacggc tgatctggcc
      301 acggcagcag ctcgcaatgc gctggccgca gccaacatca agcccgaaga cctggatttg
      361 atcattttgg ccacggatac accagagtat ctttccccgt ctacttcagt tgtggttcag
      421 caccgaatcg gggcgaaaaa tgccgggacc tttgatgtca attgtgcctg cgccgggttt
      481 gtgacggcac tcgacacggc ctgtaaattt atcgtggctg atgaggcata caccaatatt
      541 ttggtgattg gggcctatgc catgagcaaa ttcctcgatt ttaccgagaa gaaaacctgc
      601 acgatttttg cagatggcgc tggtgccgtg gtggttcagg cttcgaccga aggtccaggg
      661 ttgctggcgt cgaaactggt ggccgatgga gcgtttcacg accatatggg catttatgct
      721 ggcgggacgc atatgccggt gaccgaagaa gttttacacg aaggccgaca caccaaagtc
      781 cgatttgcca agaaatatcc acccgagatc aatcttgaag gctggccggc aatcattcga
      841 gatgttctgg ccaaggcgaa actccagttg gacgatgttg atttctttgt gtttacccag
      901 gtgaatttga gcaccattaa aatcgtgatg cagacgctgg agcttccctg ggagaaaacc
      961 cacaccatta tggggaaatg ggggtacacc ggctctgcct gtatcccaat gtgtctggat
     1021 gatgccgtgc aggctggaaa gatcaaacct gggatgaaaa tcattttgtg tgcttccggc
     1081 ggtggcctga atatggcctg tgcgctggtg cagtggtgaa aaccagggtt ccgggttccg
     1141 ggttccgggt tccgggttcc gggttccggg ttccgggt
//
LOCUS       DAOQDO010002362         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_157496_length_1177_cov_2.165775, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002362 DAOQDO010000000
VERSION     DAOQDO010002362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157496_length_1177_cov_2.165775"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1020)
                     /locus_tag="PKZ53_27260"
     CDS             complement(316..1020)
                     /locus_tag="PKZ53_27260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44214.1"
                     /translation="MTQTGELFQPVRLRYEIKDETKLRAAFSKLRCIDVDPSKNRWVW
                     LFDYEARSIQLKKSEALLPTSEGSLVIGSFFIRSGNELHLEVRSIERAIAAILFFDKH
                     LGRKIAEIKYLEIVNRLFDGPGPVPDSLSGYFDALDEAAQVDPNELVKQIKARQDQTD
                     DPNLKQEIASIFIDELSRRPEPEVERLPVRFYEEGIKPIEMSLRLRQIVATQHWQGNP
                     NYTAMDAIRGMLTGRR"
     gene            complement(1111..1176)
                     /locus_tag="PKZ53_27265"
                     /pseudo
     CDS             complement(1111..1176)
                     /locus_tag="PKZ53_27265"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
BASE COUNT          290 a          284 c          280 g          323 t
ORIGIN      
        1 agctgagcaa tttatcgaag gacttcggaa agccatcgaa atcgccagct ctattgccgg
       61 ctcagaaaca cctgaagatt gatgaggaaa ggttccttcc atggagcacc ggcaccctac
      121 tggcaactga ttggaaatgg accacctgtc gctggcaaga tactgacgct cccaggaaat
      181 aggtatctaa aaaactataa gcaccttgtc ataagtttcc tgagatattg gatttggtca
      241 gtgcttgatt gttttccgtg gcttccgtgg cttccgtggt taaaatgtct tgaaattttc
      301 ggcaagctat acttatcacc tgcgccccgt caacattccc cgaatcgcat ccatcgcggt
      361 gtaattgggg tttccctgcc aatgctgggt cgccacgatt tgtcggagtc tcaacgacat
      421 ctcaattggt ttaatccctt cctcataaaa acgaactggt aagcgttcga cctcaggttc
      481 tggccggcgt gacaactcat caataaatat tgaagcgatc tcctgtttca ggttggggtc
      541 atccgtttgg tcctggcgtg ctttaatctg tttcaccagc tcatttgggt caacctgggc
      601 tgcttcatcc agggcatcaa agtagccaga aagtgaatct ggaactggcc caggaccatc
      661 aaacaaccgg ttcacgattt ccagatattt gatttccgca atcttccgcc caaggtgttt
      721 gtcaaaaaac agaatggcgg caatcgccct ttcaatggag cgtacttcca ggtggagttc
      781 gttgccggaa cggataaaaa acgaaccaat caccagcgaa ccctctgaag taggaagtaa
      841 agcctcggat tttttcaact ggatgcttcg ggcttcgtaa tcaaacagcc agacccagcg
      901 gtttttggaa ggatcaacgt caatacacct taattttgaa aaagcagcac ggagcttcgt
      961 ctcatctttg atttcatatc ggagcctgac gggttgaaag agctctccgg tttgtgtcat
     1021 ccaaggcgcg ttttctggtg gtgtcgaagg tgatgggtct gttttttcgg gttttggtgg
     1081 ggaaggacga gcatctctaa ccggtttgcc atagcaacaa accttatatt ttttgccgct
     1141 gccacacgga catggttcat ttcggccaat tttcata
//
LOCUS       DAOQDO010002363         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_157690_length_1176_cov_2.887600, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002363 DAOQDO010000000
VERSION     DAOQDO010002363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157690_length_1176_cov_2.887600"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1176
                     /locus_tag="PKZ53_27270"
     CDS             159..>1176
                     /locus_tag="PKZ53_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNJ44215.1"
                     /translation="MKVGIVGFAGSGKTTIFNALTGLRAEVGGYGGKDKANLGIIKVP
                     DARIEALAEIYHPKKKTLAEISFIDVAGPEATEEKSNTGLDSKIVNNMREVEALVHVI
                     RAFDNPSLGQSPDPVRDIVNFDSELILADLIIVENRLNRMKKEKANPNEKALFEQCKE
                     ALESEKPLRSIEFGEEDLQLLSGFRFLSQKPLLLLLNVSEGDIAAGTPASVQASATER
                     GLTVITMCGLAEMDIAELAPEEQADFLQALGISEPARDRFIRAAYGMLDLISFLTAGE
                     DECRAWTIRRGTKAQRAAGKIHSDIERGFIRAEVVAFDDFMHYKTEAKCKEAGKLRLE
                     GKEYL"
BASE COUNT          329 a          275 c          314 g          258 t
ORIGIN      
        1 tgaaaagcca aaggaacagc caaaagcgaa aagcactgat ggcgaatcac cggcagaaaa
       61 agtgagtccg tagtccggat ggcaatgaga tacggcgtgg aactcaccgg accacgcctt
      121 tgaattttga ttttttgtaa ggattgagat aaacacccat gaaagttgga atcgtaggct
      181 ttgccggatc tggaaaaacc actatcttta atgctctgac agggttacga gctgaggttg
      241 gtgggtatgg cggaaaagac aaagccaatt taggcattat taaagtgccg gatgcccgca
      301 ttgaggcact ggcagagatt tatcacccca agaagaaaac cctggcggaa atttctttta
      361 ttgacgtggc cggccctgaa gccacagaag aaaaaagcaa caccgggctt gattcaaaaa
      421 tcgtcaacaa tatgcgcgaa gtcgaagccc tggtacacgt cattcgcgcc tttgacaacc
      481 caagcctcgg ccagtcacca gatccggttc gggatatcgt caactttgat tcggaattga
      541 tcctggcgga tttgatcatt gtcgagaacc gcctcaaccg aatgaaaaag gaaaaggcca
      601 atcccaatga gaaagcgctg tttgaacagt gcaaagaagc ccttgaatcg gaaaaaccgt
      661 tacgcagcat tgagtttggt gaagaagacc tgcagttgct gtctgggttt cggtttctgt
      721 cacaaaaacc actgttgctc ttactcaatg tcagcgaagg cgatattgca gccgggacac
      781 cagcatcggt tcaagcttca gccaccgaac gtgggttgac cgtgattacg atgtgcgggc
      841 tggccgaaat ggacattgcg gagttagcac cggaagaaca ggctgacttc ttacaggctc
      901 ttggtatttc cgagcccgcc cgtgaccggt ttatccgcgc ggcctatgga atgctggatt
      961 tgattagctt cctcacggct ggcgaagatg aatgccgcgc ctggacaatt cgccggggca
     1021 ccaaagcaca acgtgccgcc gggaaaatcc actctgacat cgaacgaggt tttattcggg
     1081 ctgaagtagt tgcttttgat gactttatgc actacaagac cgaagctaaa tgtaaggaag
     1141 ccggcaagct ccgcctcgaa gggaaagagt atctgg
//
LOCUS       DAOQDO010002364         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158316_length_1174_cov_2.065237, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002364 DAOQDO010000000
VERSION     DAOQDO010002364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158316_length_1174_cov_2.065237"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..>1174
                     /locus_tag="PKZ53_27275"
     CDS             174..>1174
                     /locus_tag="PKZ53_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ44216.1"
                     /translation="MLSPKGKYMVNQPDLTRLWEAGTGKPISDVTGSGQVLGFSPDER
                     LVLRQGNGKGGPSLWEIASGCTVRQFARNQLIIRRLLVSPDGRYLILVGPDDQYKDHQ
                     VVWDLATGQVVQHVNDANIDDLTFHPLRRCWATVDRTQKITLWDVPSGRELRSWKADS
                     KGVTTMAFSPDGQYLITANSKVSGCTIWEVATGHELRTIAQEQSDIWLISFTLDSSGI
                     IASNDKQTRIWDLTTGAQLPTFNHQSIDLSSSIISRDRRYSILNYGRPPYKLWQIENG
                     REIATFDGANEEIWSFVISHNEKALLTGSRTGKLQMWDVETGKELWAIPTGPDPIRS"
BASE COUNT          323 a          290 c          300 g          261 t
ORIGIN      
        1 caacaggaaa ggaaatccag acactgaccg gacatctcaa gcaagtcatt ttcgttgctt
       61 ttagcccaga tgggaaaaat atagtatcga ggggctctga cctcacaacg aaggtttggg
      121 acctctcaag tggacaggaa gtaaaaccca cccccaagaa tatctttcca gcgatgctca
      181 gtccgaaagg aaaatacatg gtgaatcagc ctgatttgac caggctctgg gaagccggca
      241 ctgggaaacc gatttctgac gttacaggca gtggacaggt actcgggttc agtcctgatg
      301 aaaggcttgt tttaaggcaa ggcaatggaa aggggggacc gagtttatgg gaaattgcca
      361 gtggatgtac cgttcggcaa tttgccagaa accagcttat catcaggaga ttgctcgtca
      421 gtccagatgg tcgctatctg atcctggttg gacctgacga tcaatacaaa gatcatcagg
      481 tcgtgtggga tctagccacc gggcaggttg tacagcacgt aaacgacgcc aatattgatg
      541 acctgacatt ccacccgctg cgccgctgct gggctacggt tgatcggact caaaaaataa
      601 ctctttggga cgtgcccagc ggacgtgaac ttcgctcgtg gaaggcagat tccaaaggtg
      661 ttacaaccat ggcatttagc ccggatggtc agtatctgat cacagccaac tcaaaggtga
      721 gtggctgcac gatctgggag gttgccaccg gccacgaatt gcggacaatt gcccaggagc
      781 aatcggacat atggctcatc tcattcactt tagacagttc aggcattatc gcctcaaatg
      841 ataaacaaac ccgtatctgg gatcttacaa ccggtgccca gctcccgaca ttcaatcacc
      901 aatctatcga tctatcatct tcaatcataa gccgggatcg ccgctactcg attctgaatt
      961 atggtcgtcc cccttacaaa ctctggcaaa tcgaaaacgg acgcgagata gcgaccttcg
     1021 acggtgccaa cgaagaaatc tggtcatttg tcatcagcca caatgagaaa gcccttctga
     1081 ctggaagtcg gaccgggaag ttgcagatgt gggatgttga aaccggaaag gaactgtggg
     1141 caattccaac tggaccggac ccaattcgtt cgat
//
LOCUS       DAOQDO010002365         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158350_length_1174_cov_1.854334, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002365 DAOQDO010000000
VERSION     DAOQDO010002365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158350_length_1174_cov_1.854334"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKZ53_27280"
     CDS             <1..1039
                     /locus_tag="PKZ53_27280"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ44217.1"
                     /translation="VLRNGQLLEKELVPPPAPKDEIEGCEVIYSSMVGPNGQRQRVII
                     SKPKNAQGKLPAIFFVEWLSCDTVELPASLQNGWGKMMRGVASKSGFVMMRLEKPGIG
                     DSEGPPCADCDYLTELAAHKAALQELKKLPFVDPDSVYLLGASMGGAMAPLVAQGEKV
                     KGLIISGTFTKTWLEHMLEIERRRLELSGKSRAEIAQGMVALTEFHSLVLNQKLTPEE
                     ACKLRPHLTQHWYDEPRHQYGRHIKYYQQVQDLNIEAAWEKITAPVLVVYGEFDWIMT
                     REDHLIITRIVNKNHPGTAEFVEIPGMDHHYQRFGSVQDAFDGRNGNNIAADETVTAM
                     VNWLKKTQAAN"
BASE COUNT          305 a          285 c          314 g          270 t
ORIGIN      
        1 ggttcttcga aacggccagt tgcttgaaaa agaactggtt ccgcctccgg caccgaaaga
       61 tgaaatcgaa gggtgtgagg tgatttacag ctcaatggtc ggccctaacg gtcaacggca
      121 acgcgtcatc atcagtaaac caaagaatgc ccagggaaaa ttgccagcga ttttctttgt
      181 cgaatggctg tcgtgcgaca cggttgaact cccggcaagt ctgcaaaacg gatggggaaa
      241 aatgatgcgt ggcgtcgctt cgaaatcagg gtttgtcatg atgcgactgg aaaagcccgg
      301 catcggtgat agcgaaggcc cgccctgtgc cgactgtgat tatttgaccg aacttgctgc
      361 ccacaaagcc gcgctccagg aattaaaaaa gcttccgttt gtagatcccg attcagtcta
      421 tttgctaggt gccagtatgg gcggtgccat ggcgccgctc gtagcgcagg gtgaaaaagt
      481 caaagggctc atcatttctg gcacattcac caaaacctgg ctggagcaca tgctcgaaat
      541 cgaacgccgc cgacttgaac taagtggaaa atcacgggct gaaattgctc aggggatggt
      601 ggcgttgact gagtttcatt cactggtcct caatcaaaaa ctcactccgg aagaagcctg
      661 taagctcagg ccacatctga cccaacactg gtatgatgaa ccacgtcatc agtacggacg
      721 ccatatcaag tattatcagc aagttcagga tctcaacatc gaagccgcct gggagaaaat
      781 cacagcgccg gttctggtgg tgtatggtga gtttgactgg attatgaccc gcgaagacca
      841 cctcatcatc actcgaattg tgaataaaaa ccatcctgga acggctgaat tcgtcgaaat
      901 cccaggcatg gaccaccatt atcaacgatt tgggtcggtt caggacgctt ttgatggtcg
      961 gaatggaaat aatatcgccg ccgatgaaac ggtgacggca atggtcaact ggttgaaaaa
     1021 gacacaggct gcaaactgat tcgggttccg cgcttccgcg cttccgggtt ccgggtcttg
     1081 tcattaccca aatttttccg acgaacaacg atttcaggtc aggttctttg attttatccc
     1141 ggagggatgg agcaagttta gccggtcggt tgcg
//
LOCUS       DAOQDO010002366         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158631_length_1173_cov_1.796064, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002366 DAOQDO010000000
VERSION     DAOQDO010002366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158631_length_1173_cov_1.796064"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKZ53_27285"
     CDS             complement(<1..748)
                     /locus_tag="PKZ53_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ44218.1"
                     /translation="MSYRILVVDDETGIRDSLRGVLEDEGFSVDLASTGEDCLQIIEK
                     QAYHCILLDIWLPGMDGMAVLERIRATHPETAVVMISGHGTIQMAVKATRLGAADFIE
                     KPLHIEKTLDAIRNAIKAHAQRLETASRENGAGRENGHQRDHLIIGDSVPMRALRQQI
                     ALAGPTNGRVLIYGETGTGKELVARALHHQSLRAASGSFVALNCAAIPEELIESELFG
                     HTKGAFTGATGQRRGKFEQSDNGTLFLDEIG"
BASE COUNT          253 a          313 c          295 g          312 t
ORIGIN      
        1 cgccgatttc gtccagaaag agcgttccat tgtcggattg ttcaaatttc ccacgccgct
       61 gaccagtagc cccggtgaat gctcctttgg tgtggccaaa tagctcagat tcaatcaatt
      121 cttccggaat ggcggcacag ttgagcgcca caaatgaacc tgacgctgct cgcaacgact
      181 gatgatggag cgcacgggcc accagttctt tccctgtacc ggtttcacca taaatcaaaa
      241 cccgcccgtt ggttggtccg gcaagggcga tttgctggcg gagcgcgcgc atcggcacac
      301 tgtctccgat gatcaggtga tcgcgctggt gaccattttc acgcccggcg ccgttttctc
      361 gcgacgcggt ttccaacctc tgcgcgtggg ctttgatggc attgcgaatg gcatccaggg
      421 ttttttcgat atggagcggt ttttcaataa aatcagcagc cccaagccgg gtagctttga
      481 ccgccatctg gattgtcccg tggcctgaaa tcatcaccac ggctgtctca ggatgagtgg
      541 cccggatacg ttcaagcaca gccataccat ccattccagg cagccagata tcaagtaaaa
      601 tacaatgata tgcttgtttt tcaatgattt gcaggcagtc ttcgcctgtt gaagccagat
      661 ccactgagaa accctcgtct tcgaggactc cccgcagtga gtcacggata ccagtttcgt
      721 catcaacgac caaaatgcga tatgacatgg agattcaata gtgtgtggcg tgcggccccc
      781 gtgatgccgc tttgcaaaaa tctttttcgg aaaaggtttg gttcagttat agtgcggtat
      841 gcctggttat ttggtgtgat tggctcagtg aattcaggta caaattcgac tggccgcaag
      901 aaatggtcaa tcatacaagg actgccgtaa gattcaaccg tggttttcct aactgcgtgc
      961 aaatccctgc ccaaccgatt cttgattttt cctttaacca accccatgcc tcaggccaaa
     1021 gctcagcacc agtcctgccg gtttttcagg ttccctgttt ttgggtggat gtggcgtgtt
     1081 gccgtatttg gaatactgat cgtgcccatc tggatcgggt gttgccttgg cgctgaaccg
     1141 caagctgaat cgttttctcc gtcaaatttc cct
//
LOCUS       DAOQDO010002367         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158744_length_1172_cov_3.578335, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002367 DAOQDO010000000
VERSION     DAOQDO010002367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158744_length_1172_cov_3.578335"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1172
                     /locus_tag="PKZ53_27290"
     CDS             <1..>1172
                     /locus_tag="PKZ53_27290"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44219.1"
                     /translation="LQVTATNPQTGDAIEKPVTVLPDAQEQVQTIGKLAESTTILDLT
                     APEHPVPGTLKGEVKIYPNLIDHVFEGIDGIVQRPYGCAEQTISAAYANVLVLQYLKQ
                     TGALDSPTAEKARTFTQEGIDRLIAYRLENNAFAYWGNGDPDWSLTAYAVQFLSQARE
                     FAAIDDSVLRQIQEALVREQLPNGSWPMAYQWRNGTQDKQAVAQTACIVYLLAKAGVN
                     PINQNAIAKGLAFVKPRIAASRDPYLLACYVLAASETGDLAGAQEVIPQLMQLAQVKG
                     EKVYWEQSGPTLFYGWGTAGRIETTALVLQALVTIPVANPPAEVASIINRGFQYLIET
                     KDRYGVWYSTQASLNVWRALLLLSSHEASETKSDTAESAELWVNDTLVKLVTWSAN"
BASE COUNT          311 a          298 c          297 g          266 t
ORIGIN      
        1 gttgcaggta accgcgacga atccacaaac cggagatgcc atcgaaaagc ccgtgactgt
       61 tcttcccgat gctcaggaac aggtccagac gattgggaaa ctggctgaaa gcacaaccat
      121 ccttgacctg acagcgccgg aacatccagt tcctggcact ttgaagggcg aagtcaaaat
      181 ctatcccaac ctgatagatc acgtgtttga aggtattgac ggcattgtgc agcgtccata
      241 tggctgtgct gaacaaacaa tttccgctgc ttatgccaat gtgctggttt tgcaatacct
      301 gaagcaaacc ggagcacttg actcacccac tgcagaaaaa gcccgaacgt ttacgcagga
      361 aggcatagac cgactgattg cgtatcgcct tgaaaacaat gcctttgcct actggggaaa
      421 cggtgaccca gactggtcgt taacggcgta tgcggttcag tttctcagtc aggccagaga
      481 atttgccgca attgatgact ctgttttgag acaaatccag gaagcactgg tcagagagca
      541 attgccaaac ggaagctggc caatggccta tcaatggcga aatggaaccc aggacaaaca
      601 agctgttgct cagaccgcct gcattgtgta tttactggct aaagccggag tcaacccgat
      661 caatcagaac gccatcgcca aaggactggc ttttgtaaaa cctcgaattg cagcgagccg
      721 tgatccgtac cttctggcct gttatgttct ggctgcatct gaaaccggag acctggctgg
      781 cgcccaggaa gttattcccc aattgatgca actggctcag gtaaaaggtg aaaaagtcta
      841 ttgggagcaa tccgggccaa ccttgtttta tggctgggga actgccgggc gaatcgaaac
      901 cacagcactt gttttgcaag ccctggtgac gattccagtc gcgaacccac ctgccgaagt
      961 tgcgtcaatc atcaatcgtg gctttcagta tctgattgaa actaaagatc gttacggcgt
     1021 gtggtattca acgcaggctt cactcaatgt ctggcgagca ctgctgttgt tatccagtca
     1081 cgaagcatca gaaacgaaat ctgacaccgc cgaatccgct gaattgtggg tcaatgacac
     1141 gctggtcaaa ctggttacgt ggtcagccaa tg
//
LOCUS       DAOQDO010002368         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158750_length_1172_cov_3.444047, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002368 DAOQDO010000000
VERSION     DAOQDO010002368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158750_length_1172_cov_3.444047"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..1004
                     /locus_tag="PKZ53_27295"
     CDS             399..1004
                     /locus_tag="PKZ53_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ44220.1"
                     /translation="MLSEEKRAAILKGAMEEFLAHTYAGATMDRVAQLSGVSKATIYS
                     HFGDKEALFKAIIQQLADQKPKTILKTLNPELAQSHPAQVLRQLANTMLDASLYDPEL
                     QNFMRLMIAESGRFPELARAFVSSFHQPASDQITAIFSHPALKIADPETVAQIFMHAL
                     TQYVLTQEIMHGKDVIPLDRERFVTALIELVAPGKTESARY"
BASE COUNT          311 a          281 c          277 g          303 t
ORIGIN      
        1 ggcgcattgt gaacaaaagc ctccgtgccg tgagtcataa gtcaaattca ggtttttact
       61 aaactaaact gattagttta gtaaaaaaaa tctaaactaa acagtttagt tcgtcaaggc
      121 aaatcaccac atcattctga attttgagtc tcggagggat tagctaaggc tggtcgtgat
      181 tcaattccat tccagaacgg atggaggttg tccggatgaa cgccatcgtg gaggcaccgc
      241 aacgatggct ctccgccgcc ggtgctccgc cgtccactgg gtgggaaccc tcaatctcag
      301 gcactttttc tgaaactgtg gttgttcaac ttgtctcttt tgcttgggtt gaccttcagg
      361 tattgtggca atggatttga acccagcagg aggaacttat gctctcggaa gaaaaacgtg
      421 cggctatatt aaaaggggca atggaggagt ttctggccca tacttatgcg ggcgccacga
      481 tggatcgtgt cgcccagctt tcgggggtgt cgaaagcgac gatttacagc catttcgggg
      541 ataaggaagc cttatttaaa gcgattatcc agcaactggc agatcagaag ccaaaaacaa
      601 tcttgaaaac actcaatccg gaactggcac aaagccaccc agcccaggtc ttacgccagt
      661 tggccaatac catgctggat gcttcgctct atgaccctga acttcagaat tttatgcgcc
      721 tgatgattgc cgaatctgga cgctttccag aactggccag ggcatttgtc tccagttttc
      781 atcaaccggc ttcagaccag atcaccgcta ttttttcgca cccggcttta aaaattgctg
      841 accccgaaac cgtggctcaa atctttatgc acgccctgac ccaatatgta ctgacccagg
      901 aaatcatgca tggcaaggat gtcatccccc tggatcgcga acggtttgtc actgccttga
      961 ttgagctggt ggcgcctgga aaaaccgaga gcgcaagata ttgagtagga aaaaatgagc
     1021 caaagacttt ggacctgggg ttgaaggaaa atatgaaaga agggtaactg tcttaccatc
     1081 aacctattat aaggaatgtt cgcatgcgaa cattttttgt ttttgaagga ctctcagcca
     1141 ccttttttct tcaatgcaac ttcttcagcc cc
//
LOCUS       DAOQDO010002369         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158814_length_1172_cov_2.335721, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002369 DAOQDO010000000
VERSION     DAOQDO010002369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158814_length_1172_cov_2.335721"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKZ53_27300"
     CDS             <1..269
                     /locus_tag="PKZ53_27300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ44221.1"
                     /translation="RHRYYDGFSPGYGFTGIAVALLGRNHPVGILLSAVLFGALIRAG
                     LFIDIFTDHVSKDLSVVLQAIIIICVACEGLFRGTLLKRFQPEK"
     gene            272..955
                     /locus_tag="PKZ53_27305"
     CDS             272..955
                     /locus_tag="PKZ53_27305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44222.1"
                     /translation="MRTTALIFSCLILLGFPFATLAQLALVKTASPSGLTGDRYVYRF
                     ERPEFEIARIDVQVDLKGQGNLKYQRKDEEEPTLVELNLRPESMTRLQKLFETLNFVR
                     STDTYQSSMDHPNLGTNTITVYQGELNRELTFQFTSNPSLQQISNFFRGLVTQHRRLE
                     TLTTARRYQPLDTPKQMKDLEYELKGGRIAEPEAMLELLKEFSMDDHLPLITRNHAKR
                     LVEMIEKKK"
BASE COUNT          278 a          311 c          276 g          307 t
ORIGIN      
        1 atcggcatcg ctactatgac gggttttcac caggatatgg cttcacggga attgcggttg
       61 cgcttctagg tcgcaatcat ccggtcggaa ttttgttgtc agccgttttg tttggggctt
      121 tgatccgggc cggtttgttc attgatattt ttaccgacca tgtgtcaaaa gatctgtcgg
      181 tggtgcttca agccatcatt attatatgcg tggcctgcga aggactcttt cgcgggacac
      241 ttctcaaacg gtttcaacca gaaaaatagc tatgcgcacg acagctttga tcttttcatg
      301 tctgatcctc ctcgggtttc ccttcgccac cctggctcaa ctcgccctgg tcaaaaccgc
      361 cagcccatct gggttgaccg gtgaccgata tgtgtaccgg tttgagcgcc cagaatttga
      421 aattgcccgc attgatgtcc aggttgacct caagggtcag ggaaacctga agtatcaacg
      481 caaagacgaa gaagagccaa ctctggttga actcaacctg cgaccagaat ccatgacccg
      541 gctgcagaaa ttgtttgaga cactgaattt tgtccgcagc actgatacct accagtcgtc
      601 aatggaccac ccaaaccttg gaaccaatac catcacggtg tatcaggggg aactcaatcg
      661 cgaactgacg tttcagttca cctcaaatcc ctcactccag caaatttcca atttcttccg
      721 tggtctggtc acccaacacc gacgccttga gacgctcacc actgcccgcc gataccaacc
      781 cctcgacaca ccgaaacaaa tgaaggacct ggaatatgag ctcaaaggcg gacgcattgc
      841 cgagcccgaa gccatgcttg agttgttgaa agaattcagc atggacgacc acctgccgct
      901 catcacacgc aaccatgcga aacggttggt ggagatgatt gagaagaaaa agtagggttc
      961 cgcgcttccg cgcttccggg ttttcgaatt tggtctgatt ccggactttg ccgtggaacg
     1021 atgattgaat tgaggcaggt tgtttacgat ctgtaaaatt ggtgatgctt tgggattttg
     1081 ggcgacgcaa atgtattgga tttccctgaa ccctgaaccc tgaaccctga accctgaacc
     1141 ctgtttttct tcattcttca ttcttcattc tt
//
LOCUS       DAOQDO010002370         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_158878_length_1172_cov_1.975828, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002370 DAOQDO010000000
VERSION     DAOQDO010002370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158878_length_1172_cov_1.975828"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..993)
                     /locus_tag="PKZ53_27310"
     CDS             complement(10..993)
                     /locus_tag="PKZ53_27310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ44223.1"
                     /translation="MTPTEKQQKIKQILIESLQRTPDERETCLTFLCGDDDLLRQEIE
                     TLLTESSDEELTKNLWAEIAEQAIGLLRRSQERLLPGMKLHGRYLIEKEIGHGGIGVV
                     YLARDVQVHERPVVIKMLLESSINNQWLKQKFQQESEALALIDHPGVVKVLDRGELSD
                     GRPFIVMEYIKGDTLESSLCPKGMNLDQVAFIVRQIGQALSAAHDEGIVHRDLKPTNI
                     MIQRLSGNARQIKLIDFGIAKVSQSSAHLVTDPVEAGTLAYMAIEQLLNAESSPLSDI
                     YSFGVIAYEMVTGCRPFTPHQGAEPLAVRHLFVEMQKGLFGICRERYNMLW"
     gene            complement(990..>1172)
                     /locus_tag="PKZ53_27315"
     CDS             complement(990..>1172)
                     /locus_tag="PKZ53_27315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44224.1"
                     /translation="KFFDFRGTHIKHGAVRHKVWLQKINESWYITGQQDLQTYYVETQ
                     PNPDVPQSSPPENSLR"
BASE COUNT          289 a          299 c          255 g          329 t
ORIGIN      
        1 tttttctctt taccacaaca tattgtatct ttcacggcaa ataccaaaga gccctttttg
       61 catttcgaca aacaggtgtc taaccgccaa cggttcagct ccctgatggg gagtaaaagg
      121 acggcatcca gtcaccatct cataggcaat cacaccaaag gaatagatat cactcagggg
      181 tgaggactct gcgttgagca attgctcaat cgccatgtag gcaagtgttc cggcctccac
      241 cgggtcggtt accaggtgag cagaggattg ggaaactttg gcaattccaa aatcaatcaa
      301 tttgatttgc ctggcgttcc cgctcagccg ttggatcatg atattggtcg gtttcaagtc
      361 tcgatgcaca atcccttcat cgtgggcagc gctgagcgcc tgtccgattt gccgaacaat
      421 aaatgcaacc tgatcaaggt tcattccttt ggggcaaaga gaggactcaa gcgtatcacc
      481 cttgatgtat tccatcacaa taaacggtcg cccatccgac aactctcctc gatccagtac
      541 tttaacaaca ccaggatgat caatcagcgc cagcgcttcg ctttcctgtt gaaacttttg
      601 ttttaaccat tgattgttga tcgaactctc caggagcatt ttaatgacaa ccggcctctc
      661 atgaacctga acatcacgcg ccaggtagac cactccaatt ccgccgtgcc ctatttcttt
      721 ctcaattaag tatcgcccat gaagtttcat ccctggcagc agccgctcct gactccggcg
      781 taacaaacca atggcttgtt cagcaatttc agcccacaga tttttggtca actcttcatc
      841 tgaactttca gtcaacaggg tttcaatttc ctggcgtaac aaatcatcgt ccccacagag
      901 aaaagtcagg cacgtttctc gttcgtccgg agtccgttga agcgattcaa tcaggatttg
      961 tttgattttc tgttgttttt cagtgggtgt catcgcaacg aattctccgg tggtgaagat
     1021 tgaggtacat ctggattggg ttgagtctca acataatatg tttgcaggtc ctgttgtccg
     1081 gtgatgtacc aggactcgtt gattttctga agccagacct tatgccggac ggctccatgt
     1141 ttaatgtggg tgcctcggaa atcaaaaaat tt
//
LOCUS       DAOQDO010002371         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_159182_length_1171_cov_1.784050, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002371 DAOQDO010000000
VERSION     DAOQDO010002371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159182_length_1171_cov_1.784050"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1171)
                     /locus_tag="PKZ53_27320"
     CDS             complement(<1..>1171)
                     /locus_tag="PKZ53_27320"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ44225.1"
                     /translation="PISPRAQTGLKIVGPTGQELPVVNERKDLQLKVVNSTGQPIQAT
                     NWASDSTDVARIDANTGKVAGITTGFATISAQTSQGRVQVIIAVARIKNPNKSMKVSG
                     DSTLDTQNNYYLSDPRTDVIFQAAIGNSAGPQVYAGSLNSPGFQDGSSTSAKFNNPLG
                     LAFDNRPTGGLFVADSTNHCVRKIDGQRRVTREIGAAGTPGQMTGDDISFDQMRFNSP
                     SGVAIAGGNLLVSDTANHAIWYLDFQKKRVNLFAGQPGIPGKLDGRGRSAQLNRPTGI
                     SINSEGTFLAVADEGNNAVRLMKVVSENGRLVGEVTTVGKTSSARDVDIAESRLSDRA
                     ESALVFNSPRTVSIDDLGNITVVEGNGAVSVALRPGGREPIKIELAQTGTFIEAA"
BASE COUNT          249 a          323 c          294 g          305 t
ORIGIN      
        1 ttggcagctt caataaaagt cccagtttgg gccagttcga ttttgatcgg ttctcttccg
       61 cctggtcgga gcgcaacact caccgcccca tttccctcaa ccacagtaat gtttccaagg
      121 tcatcaatcg aaactgtccg tgggctgtta aacaccagcg cactttcggc acgatcactc
      181 aaccgggatt cggcaatatc cacgtcacgt gcgcttgaag ttttcccaac cgtggtcact
      241 tcgcccacca gtcgcccatt ttcagacaca actttcatga gtcgaacggc attgtttcct
      301 tcatcggcca cagccagaaa tgttccttcg ctattgatgg aaatgccggt tgggcgattc
      361 aactgggctg accggccacg gccatccagt ttgcctggga ttcctggctg accggcaaac
      421 aggttgacac gcttcttctg aaagtcgaga taccagatgg catggttggc agtgtcggac
      481 accagcaggt ttccaccagc gattgccacc ccggatggcg agttaaaccg catctgatca
      541 aatgaaatat cgtctccggt catctgacct ggggttccag cggcgccaat ctcgcgggtg
      601 acccggcgtt gaccatcaat ttttcgcaca cagtggttgg tgctgtcagc cacaaacaat
      661 cccccagttg gacgattgtc aaaggctaac ccaagcggat tattgaactt tgctgaggta
      721 ctggacccat cttgaaaccc tggggagttt aaacttcccg catacacttg aggtccagcc
      781 gaattcccaa tcgcagcttg gaaaatgacg tcggttcgtg ggtcgctcag gtaatagttg
      841 ttttgagtat cgagggtgga atccccgctg actttcattg atttattcgg atttttgatc
      901 cgggcaaccg caatgataac ttgcacccgt ccctgtgaag tttgggccga aattgttgca
      961 aagccggttg taatcccagc tacctttccg gtattggcgt caatccgggc gacatcagtt
     1021 gaatcacttg cccagttggt cgcctggatc ggttgcccgg ttgaattgac caccttgagt
     1081 tggaggtcct ttctttcatt tacgaccggg agttcctgtc ccgtagggcc tacgattttg
     1141 agtccagttt gcgcccgagg actgatcggc g
//
LOCUS       DAOQDO010002372         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_159275_length_1170_cov_3.294170, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002372 DAOQDO010000000
VERSION     DAOQDO010002372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159275_length_1170_cov_3.294170"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKZ53_27325"
     CDS             <1..977
                     /locus_tag="PKZ53_27325"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNJ44226.1"
                     /translation="YNELGADPEDIFAEFETTAFAAASLGQVHRARLKSGERVAVKIQ
                     YPNIARTIHEDFKNLNALLWPVRLGEDWENFREQMADILHVLEQETDYLREAQRLQKA
                     RCLFQPQEGIVIPRVFGEFTTRRILTMEYLEGQHINAYLATNPSQVECDQHGERIARS
                     AFRLMNLGRMCYGDPHPGNYLFLPDGRLGMIDFGCCRDLSDEEFDYMVEMEVSCKNSP
                     EKLKGALLKSVGCGPNEELDPDLSKMLQKYIDWVWEPLRAEGTFDFGNEQYLQRGMEI
                     LMEIIGKRYTRSQPVNNWLNRNFLGIRSMALRLKARVNMRQIEEEEAP"
BASE COUNT          325 a          270 c          291 g          284 t
ORIGIN      
        1 tctataacga actgggcgct gacccagaag acatcttcgc tgaatttgaa acgaccgcct
       61 ttgctgctgc ttcactcggg caggttcatc gggcccggtt gaaatcgggc gaacgagtgg
      121 cggtcaaaat tcaatatcca aacattgccc gcaccatcca cgaagatttc aaaaacctga
      181 acgcgttgct ctggccggtt cgattagggg aagactggga gaatttccgc gagcaaatgg
      241 cagatatttt gcacgtgctg gagcaggaaa ccgactatct gcgtgaagct caacgattac
      301 aaaaagcccg gtgtttgttt caacctcaag aaggtattgt cattcctcgg gtgtttggtg
      361 aatttacaac ccgccgcatt ttaaccatgg aatacctcga agggcagcat atcaacgctt
      421 atctggccac aaatccttcc caggttgagt gtgaccagca tggcgaacgg attgcccggt
      481 ctgcgtttcg gctgatgaat ctggggcgaa tgtgttacgg agaccctcat ccgggaaatt
      541 atctgttttt gccggatggc cgacttggaa tgattgactt tgggtgctgt cgcgaccttt
      601 cagatgaaga gtttgactac atggttgaaa tggaagtgag ctgcaaaaac agccctgaaa
      661 aactcaaagg tgcgcttttg aaatcagttg gttgtggccc aaatgaggaa ctggatcctg
      721 acctgagcaa gatgcttcaa aagtacattg attgggtctg ggagccgctt cgggctgaag
      781 gaacatttga ttttggaaat gaacaatacc tgcagcgggg gatggaaatc ctgatggaaa
      841 tcatcggcaa acgctatacc cgctcacagc cggtcaacaa ctggctcaat cgcaattttt
      901 tgggcattcg gtcaatggcg ctccggttaa aagccagggt taatatgcgc caaattgaag
      961 aggaagaagc tccttagtca gtagttgata agtcagtagt cagtagtcag tagtcagtag
     1021 accaaacccg gacatttcaa tgagttcatt tccgagaaaa aatagtttgt aaccagcaca
     1081 aatagaaccc aatacacttg acacaaattc ccaaccccca acactcgact actgactact
     1141 gactactgac tactgactac tgactactga
//
LOCUS       DAOQDO010002373         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_159551_length_1169_cov_3.769300, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002373 DAOQDO010000000
VERSION     DAOQDO010002373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159551_length_1169_cov_3.769300"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /gene="rsmD"
                     /locus_tag="PKZ53_27330"
     CDS             complement(<1..476)
                     /gene="rsmD"
                     /locus_tag="PKZ53_27330"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNJ44227.1"
                     /translation="MRIISGQFKGRRIRTVDGLSVRPTSDRLRETLFNILSPQVVDAR
                     FLDLCAGSGAVGIEALSRGASQVTFVENARHAIRMLIDNLTALDIDDGVDLIQRDVLK
                     ALKQLITEDRSYDIIFFDPPYASPVYQPVMKQLGESNVLSPEGWLVVEHHAKQTLL"
     gene            complement(567..929)
                     /locus_tag="PKZ53_27335"
     CDS             complement(567..929)
                     /locus_tag="PKZ53_27335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44228.1"
                     /translation="MTHEEMERTVQFLLDQTAKLTTDLEWVTSSHASLLHNVDRLATV
                     VSKLANAVEKVIDLQEKVAVREAARPMQQAEQQRQVDEKLEDLSQRVDIFITTVERYI
                     SEKQHRRRISKKLPSDSA"
BASE COUNT          280 a          281 c          306 g          302 t
ORIGIN      
        1 tccagcaatg tttgtttggc gtggtgttca accaccagcc atccttcggg tgacaacaca
       61 ttgctttcac ccagttgttt catcactggt tgatagaccg gtgaggcata cggcggatca
      121 aaaaaaatga tgtcgtaact gcggtcttcg gtaatcaatt gcttgagtgc tttgaggaca
      181 tcgcgttgga taaggtcaac tccgtcgtca atgtcgagtg ccgtcagatt gtcaatcaac
      241 atgcggatgg catgccgggc attttcaaca aaggtgacct ggctggcgcc tcggctcaag
      301 gcttcgattc cgacggcacc tgaaccggca cacaaatcca gaaagcgggc gtccacaacc
      361 tgcggtgaca agatattaaa gagtgtttca cgcaatcggt cagaagttgg tcggacactg
      421 agtccatcca cggttcggat ccgtctgcct ttgaattgtc cggaaatgat gcgcatggtt
      481 tttgcaaaaa cgccgcacgt tagcacaaat cagggtgatt tgcgattttt tgcctgggga
      541 aacgctggct catttccagg tggaagctag gcactatcag atggcaattt ttttgatatt
      601 cggcggcgat gttgtttttc cgagatatag cgttcaaccg tggtaataaa aatatccact
      661 cgctgtgaca aatcttccag cttctcgtca acctggcgtt gctgttcagc ctgttgcatc
      721 ggtctggctg cctcacggac ggcgaccttt tcctgaagat caatcacttt ttcgacggca
      781 ttggcgagtt tgctcaccac tgttgccagc cgatccacat tatggagcag tgacgcgtgc
      841 gaactggtta cccactccag atcagtggtg agtttggctg tttgatccag caaaaactgg
      901 actgtccgct ccatttcttc gtgagtcatt ggcagtcacc gaaaatctga aaattcgggt
      961 gaggttgatc cagggcagga ttgaacccca tttgtaaacc acctggtttc aaaagcgcaa
     1021 atcgttgtga tgaaagttga accaaatgag caaaaacaat tgaggtttcg gggccagacc
     1081 gtgtgctgac cacagcgaaa cctcaactca agatgcagac tggtgattca gagtgaatag
     1141 gatgccgcgg cccgatgttg ggcctggcg
//
LOCUS       DAOQDO010002374         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_159615_length_1169_cov_2.607720, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002374 DAOQDO010000000
VERSION     DAOQDO010002374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159615_length_1169_cov_2.607720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1169)
                     /locus_tag="PKZ53_27340"
     CDS             complement(174..>1169)
                     /locus_tag="PKZ53_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ44229.1"
                     /translation="IAEVISGWTGIPVGRMVTDEINAVLALKDRLEQRVVGQSQALES
                     IAQRIRTSRASLTDPRRPVGVFLLVGPSGVGKTETALTLAETLYGGERNLITINMSEY
                     QEAHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVMELFYQVFD
                     KGILEDGEGREIDFKNTVILLTSNVASDSMMLLCTDPEEPRPEPEVLVEAIRPALIKT
                     FKPALLGRMVVVPYYPIADDVMRQIVTLQLGRIGRRLTENHNAAFSYDEAVVDEIVKR
                     CTEVESGARNVDHILTGTMLPEMAEEVLAKMAAGKTLATVHVSLNETGQFLYQVQ"
BASE COUNT          278 a          315 c          288 g          288 t
ORIGIN      
        1 gaaaaggatg acagaatgaa agtctcaaat tggtgagatt gaatatggag taaaaatatt
       61 tacaccagag tatcattcat tggtttgcat ttcgatgcaa aacaaaacgg gcggaaacca
      121 gtgtagtttc cgcccgtttt tccacaagct taagcttgaa ctctgaactt cttttattgc
      181 acttgataca aaaattgacc ggtttcgttc aggctgacgt gaacagtggc cagcgtcttg
      241 ccagcagcca ttttggccaa gacttcttcc gccatttccg gcagcatggt tccggtcagg
      301 atgtgatcta cgttgcgggc gccgctttcg acttcagtgc agcgcttgac gatttcatcc
      361 accacagctt catcataact aaaagccgcg ttgtgatttt cggtcaaccg ccgaccaatc
      421 cggcccagtt gcagagtcac aatctggcgc atcacgtcat cagcgattgg gtaatagggc
      481 acaaccacca ttcgacccag taaggccggt ttgaacgtct tgatcaaggc agggcggatg
      541 gcttcaacca gtacttctgg ttccggtcgt ggttcttccg gatcggtaca cagcagcatc
      601 atcgaatcgc tcgccacgtt tgaggtcagc aaaatcaccg tgttcttgaa gtcaatttcg
      661 cggccttcgc catcttccaa aattccctta tcaaagacct gataaaagag ttccatcaca
      721 tccggatggg ctttttcgac ttcatcaagc agcaccaccg aatagggttt gcggcgaacg
      781 gcttcggtca agacaccacc ttcgccatat ccgacatagc ctggaggcga acctttgagc
      841 gatgaaaccg tgtgggcttc ctgatattcg ctcatattga ttgtgatcag gttgcgttcg
      901 ccgccataga gcgtttcagc cagtgtcagt gcggtctcgg tttttccgac gccgctgggc
      961 ccaaccagga ggaaaactcc aaccgggcgc cggggatcag tcaaactggc tcgcgaagtc
     1021 cgaattcgct gggcaatact ttccagggcc tggctctggc ctacaacacg ctgttcgaga
     1081 cgatctttga gcgccagcac ggcattgatt tcatcggtca ccatgcgacc aaccgggatg
     1141 cccgtccagc cggaaatgac ttcggcaat
//
LOCUS       DAOQDO010002375         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_159636_length_1169_cov_2.307002, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002375 DAOQDO010000000
VERSION     DAOQDO010002375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159636_length_1169_cov_2.307002"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKZ53_27345"
     CDS             complement(<1..785)
                     /locus_tag="PKZ53_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HNJ44230.1"
                     /translation="MTYPIKSPRKLIEVALPLDAINAAAAREKSIRHGHPSTLHLWWA
                     RRPLAAARAVIFAQLVNDPGYQQGGGFKYGKNKKEAAIERKRLFKIIEDLVLWENTTN
                     EEVLERARAEIRRSWREVCELNKDHPQAAELFNPEKLPALHDPFAGGGSIPLEAQRLG
                     LEAYASDLNPVAVLINKAMIEIPPKFAGRPPVNPESRKETRLIAREWQGAQGLAEDVR
                     YYGQWMRDEAFKRIGHLYPPVEITEEMAQDRPDLKPLIGQKLTV"
     gene            complement(807..>1169)
                     /locus_tag="PKZ53_27350"
     CDS             complement(807..>1169)
                     /locus_tag="PKZ53_27350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44231.1"
                     /translation="IKFSRTGVPEPNVESCEQVNFTNNNHPDTVTLVAQTITHLNLGC
                     TRLNKTRHSLIINLEEQIRDLRTKYPGVAPQEVLSLLAQRLFSSNTDSPWSQFFSLIR
                     WRLGDVGEQHLRSIGYEG"
BASE COUNT          273 a          284 c          296 g          316 t
ORIGIN      
        1 acagtaagtt tctgaccaat cagaggtttc aggtcagggc gatcctgggc catttcctcg
       61 gtaatttcga ctggtggata aagatgacca atgcgtttga acgcttcatc acgcatccat
      121 tgaccataat accgaacatc ttcggccaga ccttgagccc cttgccactc acgagcaatc
      181 aaccgggtct ctttccgtga ttctgggttg actggtggtc gcccggcgaa tttgggcgga
      241 atttcaatca tggccttgtt gatgagcacg gccaccgggt taagatcgga ggcataggct
      301 tccagtccga gtcgctgtgc ttcaagtgga atcgaaccgc ccccagcaaa tggatcgtgt
      361 aatgcaggga gcttctcagg attaaataat tcagcagcct gtggatgatc tttgttgagt
      421 tcgcagactt cgcgccagga acggcgaatt tcggcccggg cacgttccag aacttcctcg
      481 tttgtcgtgt tttcccaaag tactaaatct tcgataattt tgaagagccg ctttcgctca
      541 atcgcagctt cctttttgtt cttcccatat ttgaaccctc caccctgctg atagccaggg
      601 tcattgacaa gctgtgcgaa gatgaccgcc cgtgccgccg ccaatggtcg ccgtgcccac
      661 cacagatgca gcgtacttgg atgaccatgc cggatggatt tttcacgtgc cgccgcagcg
      721 ttgatagcat caagcgggag agcgacttca atcagtttgc gtggggattt gataggatag
      781 gtcataaagc aatgtgtttg ttttggttag ccttcgtagc caattgaccg cagatgttgt
      841 tccccaacat cacctaatcg ccaccggatg agcgaaaaga actgcgacca cggagaatca
      901 gtgtttgagg aaaacagacg ctgggcaaga agggagagaa cttcctgcgg tgcaacgccg
      961 ggatatttcg ttcgtaaatc cctaatctgt tcttcaaggt ttattatgag actatgccga
     1021 gttttattta gtctcgtaca gccaagattc aaatgtgtga ttgtttgagc aaccagtgtg
     1081 accgtgtcag ggtggttgtt atttgtgaaa tttacctgct cacaactttc tacattgggc
     1141 tcaggaactc ctgttcgaga aaatttaat
//
LOCUS       DAOQDO010002376         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_159868_length_1168_cov_2.775382, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002376 DAOQDO010000000
VERSION     DAOQDO010002376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159868_length_1168_cov_2.775382"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKZ53_27355"
     CDS             <1..404
                     /locus_tag="PKZ53_27355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44232.1"
                     /translation="FSSNREGSWDIFCAELKGDSVVQVVKVAGELNTRFDEWPSYVDP
                     QERFMLLSSTRPGGAGGDDLYIAVRENGAWSAARNLGPRVNSPGFEDGASLSPDQKTL
                     YYSSRNAQSLSQIYQIRVAELPGQFSSILAD"
     gene            complement(377..880)
                     /locus_tag="PKZ53_27360"
     CDS             complement(377..880)
                     /locus_tag="PKZ53_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ44233.1"
                     /translation="MHLTYRTDYSIRVLLYLALYPDRLVTTKEISDVYGISKNHLVRV
                     VLHLNKCNFIENHPGRTGGIKLARPADSFTIGEVVRLTEPDFHLVECFNEVDNTCPII
                     SVCGLKGVLRKANSAFLGVLDNYTLADVLKGSPQKLKSFLQIGTLVPERPRSTTKKRQ
                     SAKIEEN"
     gene            complement(914..>1168)
                     /locus_tag="PKZ53_27365"
     CDS             complement(914..>1168)
                     /locus_tag="PKZ53_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HNJ44234.1"
                     /translation="ELAAQFPQFQYLPTVLNGDCQDDQPVLTGSLDTVVVSRVPQLKA
                     MRAFLCGDPDIVNRLRKKFFLAGLSLRDIYADAFVPSSPS"
BASE COUNT          292 a          290 c          306 g          280 t
ORIGIN      
        1 atttttcgtc caatcgcgaa gggtcctggg atatcttttg tgccgaactc aaaggtgatt
       61 ccgtggttca ggtcgtcaaa gtggctggtg agcttaacac ccgctttgat gaatggccga
      121 gctatgttga cccacaggag cgatttatgt tgctcagttc aacccgcccc ggcggggctg
      181 gcggcgatga cctctatatc gcagtcaggg aaaacggtgc gtggtcggca gcccgcaacc
      241 tgggcccgcg ggtcaactcc cctgggtttg aagatggtgc cagcctcagc cccgaccaaa
      301 aaacactgta ttactcaagc cggaatgctc agtccctgag ccaaatatat caaattcgag
      361 tggccgagtt gcccggtcaa ttttcttcga ttttggctga ctgacgtttt ttggtggttg
      421 atcggggacg ctctggcacc aatgtcccaa tctgtaaaaa cgacttgagt ttttgtggtg
      481 accctttgag tacatcggcc agggtatagt tatcgagtac acccagaaac gcactgttgg
      541 ctttgcgcaa tacacctttg aggccacaga cgctaataat tgggcaggta ttgtcaacct
      601 cgttaaaaca ttcgaccagg tgaaaatcgg gttcagtgag ccgaacgact tcgccaatcg
      661 tgaatgaatc agccggtcgt gccagtttaa tgccaccagt acggccagga tggttttcga
      721 taaagttaca tttattcagg tgcagaacca cccggacgag gtgatttttt gagattccat
      781 agacgtcgct gatttctttg gtggttacca gccggtctgg atagagtgcc agatacaaca
      841 gcactcgaat tgaataatcg gttcgatagg tcaaatgcac gatcagaggt ctcctctgaa
      901 aaaatgaatg cctttacgag gggctacttg ggacaaaggc atcagcataa atatcgcgca
      961 atgagaggcc cgccagaaag aactttttcc gtaatcggtt cacaatatcc ggatccccac
     1021 acaaaaacgc ccgcatggcc tttaactggg gaacacggga caccacaacc gtgtcgagcg
     1081 agccggtcaa aaccggttga tcgtcctggc aatcaccgtt taaaaccgtc ggcaggtact
     1141 ggaactgagg aaactgagcc gcgagttc
//
LOCUS       DAOQDO010002377         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_161055_length_1164_cov_2.056808, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002377 DAOQDO010000000
VERSION     DAOQDO010002377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161055_length_1164_cov_2.056808"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1164
                     /locus_tag="PKZ53_27370"
     CDS             <1..>1164
                     /locus_tag="PKZ53_27370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44235.1"
                     /translation="SEANFKRLFPGIPGQRVFLIDAAKPTETSALLEDRLSDYGFDVQ
                     DAGAKLASFHQVENTYLSTFQALGALGLLLGTVGLAAVLLRNVPPTTANYIQRAGRAG
                     RRTESAAYVLTFCQRRPHDLYHFQDPSQLVAGQVKTPQIWISNEKIVRRHMQSVLIAD
                     FFRKYAEQWSAMRNIGGFFSIESSTLIADWLKDHAQSHPPQVQSALMRIVPEDLQAEI
                     GLENWGWLRKDDEDGMLDILERMTDEVKTDQVLYLTAIKDAADGKRFKAASFFQDVLN
                     TINGRQLIDFFASRNLLPKYGFPTDVVTLKTDHIEHPKARLIQLDRDLRIAISEYAPG
                     AEVVAAKTIWTGGGIHLLPKKRLPTHSYAICKICSHFQQAKEVENQEKICQECG"
BASE COUNT          300 a          295 c          292 g          277 t
ORIGIN      
        1 gtccgaagcc aatttcaaac gcctctttcc cggcattccg ggccagcgcg tcttcctgat
       61 tgacgcggcc aaaccgactg aaacgagcgc gttgctcgaa gaccgtttga gcgattacgg
      121 ctttgacgtg caagacgcgg gcgcaaaact cgccagcttc catcaggttg aaaacaccta
      181 cctctcgacg ttccaggcgc tcggcgcgct cggcttattg ctcggcactg tcggactcgc
      241 ggcagtcttg ctgcgcaatg tgccgccgac aacggccaac tatattcagc gcgctggacg
      301 ggccggacgc cgaaccgagt ctgccgccta cgtccttacc ttttgtcagc ggcggccaca
      361 tgatctgtac cattttcaag atccatccca actggttgcc ggtcaggtga aaacacctca
      421 aatttggatc agcaatgaaa aaattgttcg acggcatatg caatcggttt tgattgctga
      481 ctttttccgc aaatatgctg aacagtggtc cgcgatgcga aacataggtg gatttttttc
      541 gattgaatcc agtaccttga tagcagattg gttgaaagac catgcccaat cacatccacc
      601 tcaggttcaa tcggccttga tgagaattgt cccggaagac cttcaggcag aaattggatt
      661 agaaaattgg ggatggctca gaaaagacga cgaagatggc atgttggata ttctggaacg
      721 aatgaccgat gaggtaaaaa ccgaccaggt cttgtattta actgccatca aagatgctgc
      781 ggatgggaag cgatttaaag ccgccagttt ctttcaggac gtgctcaata ccatcaatgg
      841 gcggcagttg atagattttt ttgcttcacg gaatttgctc ccgaaatatg gatttccaac
      901 ggatgtggtc accctgaaaa ctgatcatat cgaacacccc aaagcgcggc tgattcagct
      961 tgatcgtgac ctgcgaattg ccatttcaga atatgcgcca ggtgctgagg tcgtggcagc
     1021 caaaacaatt tggacgggag gaggaattca cctccttccg aaaaaaaggc ttcccaccca
     1081 ttcttatgcc atctgtaaga tttgctcaca tttccagcag gcaaaagaag ttgagaacca
     1141 ggaaaagatt tgccaggaat gcgg
//
LOCUS       DAOQDO010002378         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_161252_length_1163_cov_2.753610, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002378 DAOQDO010000000
VERSION     DAOQDO010002378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161252_length_1163_cov_2.753610"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1077)
                     /gene="flgA"
                     /locus_tag="PKZ53_27375"
     CDS             complement(58..1077)
                     /gene="flgA"
                     /locus_tag="PKZ53_27375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNJ44236.1"
                     /translation="MTTHPSAHPQSVNRFCQILTGVGLMWCLICGLVPVCAAQADRAQ
                     VRIGSEILAEKDRLTLGDIAEIAASTPESDEALKSVSLGYSPRLGTVRELPRDRVLLA
                     IQSAGFTDETVTVYAPPVILIRRAAQTIDLAYLKAAVEEAVLPDLEINGVTARLVRLD
                     LPPVVQVPTGKTEIRAQVRNVRDIYYPFTVGIDIVVDSLVVRRFQATAQVEAFAAVLV
                     ANENLAAGQRLTRTVVSIAPCRLAQLIRLYFRDPGAVRGMALRKPVSKGSPITTDLVF
                     PDTVIKPGDQVRIIGKTGTVTIEVMGEARTGGRIGDRIQVKNSQSGVILQATVVDEGM
                     VQVVL"
     gene            complement(1074..>1163)
                     /locus_tag="PKZ53_27380"
     CDS             complement(1074..>1163)
                     /locus_tag="PKZ53_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNJ44237.1"
                     /translation="IAAQRAYETNSKVITAADRMLSTINQAVS"
BASE COUNT          260 a          321 c          333 g          249 t
ORIGIN      
        1 ccccagaagt cgtgggaaca gttgaatcga aaaattggat ttcatatgga ttccttttta
       61 gagcaccacc tgcaccatgc cttcgtcaac caccgtcgcc tgaaggatga cacctgactg
      121 ggaatttttg acctggattc ggtcaccgat gcggcctccg gtgcgggcct ctcccatcac
      181 ttcaatggtc accgtgccgg ttttgccaat gattcggacc tgatcgccag gtttgatgac
      241 agtgtccgga aaaaccagat cggttgtaat cggactcccc ttggaaacag gttttcggag
      301 ggccattccg cgaacggcac ctggatctcg aaaataaagc cggatgagtt gcgcaagccg
      361 gcagggtgca atgctgacca cagttcgggt caggcgttgg ccggcggcca ggttttcgtt
      421 tgcgaccaga acggcggcaa aggcttcgac ctgagcggtg gcctgaaagc gcctgaccac
      481 cagcgaatca accacgatgt caatgccgac ggtaaatggg taatagatat cgcggacatt
      541 gcgaacctgg gcccggattt cagtttttcc ggtgggcacc tgcaccacgg gtggcaggtc
      601 gagccggacc agtcgagccg tgaccccgtt tatctccagg tcgggcaaca cggcttcttc
      661 gaccgccgct ttgaggtacg ccagatcaat cgtttgggcg gctcgtcgaa tcaaaataac
      721 cggcggggca taaacggtga cggtttcatc ggtaaaccca gccgattgaa tcgccagcag
      781 tacccgatcc cgaggtaact cgcgaaccgt gcccagtcga ggtgagtatc ccagggaaac
      841 cgattttaag gcttcgtcgc tttccggcgt actggcggca atttcagcaa tatcgcccag
      901 ggtcagccgg tctttttcag ccagaatttc agagccaatg cgaacctgag cgcggtctgc
      961 ttgagccgca caaaccggga ccaggccaca tatcagacac cacatcaacc ccacacccgt
     1021 cagtatctgg caaaaccggt tcacagattg ggggtgagca ctggggtggg tggtcatgag
     1081 acagcctgat tgatagtgct gagcattcga tcagccgcgg tgatgacttt ggaatttgtt
     1141 tcataggccc gttgggcggc aat
//
LOCUS       DAOQDO010002379         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_161358_length_1163_cov_1.866426, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002379 DAOQDO010000000
VERSION     DAOQDO010002379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161358_length_1163_cov_1.866426"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKZ53_27385"
     CDS             <1..583
                     /locus_tag="PKZ53_27385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44238.1"
                     /translation="VPLQANGSPRSYETHVFDARTGNHLFSLPPVDTQIYSFSVIGLS
                     DLRMVRLVYRKNSVPTNAQIQPAYVEVFSASGVLERTIALEKAGEFQGVTEYSLNGES
                     SAGKVLVLSETESYQLRQMTRFCVIDCNSGEIQEKLRSHSLFQGTAAWFQRDAAKENA
                     TRLFLERPQGQPKSLIYFNPETGEKRMILGAKN"
     gene            680..>1163
                     /locus_tag="PKZ53_27390"
     CDS             680..>1163
                     /locus_tag="PKZ53_27390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44239.1"
                     /translation="MKSLNIILALANRQLLENRPIFALALVGGLATLALPFVIGTPPK
                     AADHREFLENIRAGAQVLGMVVSYGVAFYLGISLFGRELSEKRMGFFFSRPISTNALW
                     IGKLLGAWLLIASSLLLVCLPAILMDANPFLLREKMSWPVVMSLVGALPFLFCLGLTA
                     S"
BASE COUNT          273 a          300 c          289 g          301 t
ORIGIN      
        1 ggttccactt caagctaatg ggtcacctcg atcttatgaa acccacgttt ttgatgcccg
       61 cactggaaac catttgtttt cactcccccc agtagatact cagatttatt ctttttctgt
      121 cattggactt tccgacctcc ggatggtcag actggtgtac agaaaaaact cagttccaac
      181 gaatgcgcag attcagcccg cttatgttga agtcttttca gcctccggcg tgcttgagcg
      241 aaccattgcg ctggaaaaag caggcgaatt ccagggggtt accgagtaca gcctcaacgg
      301 agaatcctca gccggaaaag tccttgtact gtcagaaacc gaaagttatc agctacgtca
      361 aatgaccaga ttttgcgtca ttgactgtaa tagcggggaa atccaggaaa aattacgatc
      421 tcactctctc tttcaaggaa cggcagcctg gtttcaacgt gatgcagcga aggaaaatgc
      481 aacccggctt ttccttgaac gtccacaagg acaaccgaaa tcgctgattt atttcaatcc
      541 ggaaacaggc gaaaaacgaa tgatcctggg tgcgaaaaac tgatcacgaa gtctcaaatc
      601 acccggaaga tgcccgccaa atccacatcg gcgggcatct tccgattttg caccagcaat
      661 tctgtggaga caaaccacca tgaaaagcct gaacatcatc ctcgccctgg ccaatcgcca
      721 actccttgag aatcgcccga tttttgcact ggcattggtc ggtggactgg ccacgctggc
      781 gcttccgttt gtgattggga ctccacccaa agccgctgat caccgggagt ttctggagaa
      841 tattcgagct ggggcccagg tgctgggaat ggtggtaagt tatggtgtcg ccttttacct
      901 gggcatttcc ctgtttggcc gcgagctttc tgaaaagcga atgggatttt tcttctcacg
      961 tccaatttcg accaatgccc tctggattgg gaaactgctt ggcgcgtggc tgctgattgc
     1021 cagttcgctg ttgctggtgt gtttgccggc aatcctgatg gatgccaatc cttttctgct
     1081 gcgggaaaaa atgagctggc cggtggtcat gtctctggtg ggagccttgc ccttcttatt
     1141 ttgtctggga ctgaccgcga gca
//
LOCUS       DAOQDO010002380         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_161678_length_1162_cov_1.680217, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002380 DAOQDO010000000
VERSION     DAOQDO010002380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161678_length_1162_cov_1.680217"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PKZ53_27395"
     CDS             complement(<1..750)
                     /locus_tag="PKZ53_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44240.1"
                     /translation="MDTVGLIQNPLALAHYRFGTVQTSGPLSVIPIFGPDRQGEFVGP
                     RSGLKLSQVKGYGNLELNNPAARGVAIVPLHMGYIQENAQNHCLCRSGFIAAGQKVMF
                     DDACCVQQAQGGYLTEREQWFFVLPLALREAAFQRRRIQNYSKLWPSIRELCQQFHLE
                     DRGHLEQIISRKRAYLNQYQSRFELLDGQIGAAFFLNHRLVGVEIAPTADYFREVWMG
                     LICFCYGTSAMYLEQHAGWELTPKPLEGASVA"
     gene            complement(732..>1162)
                     /locus_tag="PKZ53_27400"
     CDS             complement(732..>1162)
                     /locus_tag="PKZ53_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ44241.1"
                     /translation="TRTITVGSQGDPPIPNGPTDLATAVLDAVADHPDLVVVLSDGYE
                     NTCQGDLAAVVAALPAAGCDTPVVFCHSKFSHSDDLQLRRPANNLTALEFWHEREFPS
                     LVERLWCLARGNQGWETLKDHWRTELFRWHKELMVWTRLD"
BASE COUNT          271 a          330 c          308 g          253 t
ORIGIN      
        1 cgcgactgat gctccttcaa gtggtttggg cgtcaattcc cagccggcgt gctgctccag
       61 atacattgcc gaggtgccat aacaaaagca aatcagtccc atccagactt cgcggaaata
      121 gtcggcggtt ggcgcaattt caaccccgac cagccggtga ttcagaaaaa aagcagcgcc
      181 aatctgccca tccaggagtt caaagcggct ctggtattga ttcaagtagg ctcgcttccg
      241 gctaatgatt tgctctaaat gacctcggtc ttcaagatgg aattgctggc acaactcacg
      301 aatggatggc cagagtttgc tgtaattctg gatccgacgg cgttggaatg cagcctcccg
      361 caatgccagc ggaagaacaa aaaaccattg ttcacgttcg gtcaggtaac caccctgagc
      421 ctgttgcaca cagcaggcgt catcaaacat cactttctga cccgccgcga taaatcctga
      481 ccggcacagg cagtgatttt gggcattttc ctgaatatag cccatgtgca gcggcacaat
      541 cgccacacct cgggctgccg ggttattcaa ctccaggtta ccataaccct tgacctgtga
      601 aagttttagc ccgcttcgtg ggccgacgaa ttcgccttgt cggtcaggtc caaaaatcgg
      661 aatgaccgac aacggcccgg aggtttggac cgtgccaaac cggtagtgag ccagcgccag
      721 cgggttttga atcaatccaa ccgtgtccat accatcaact ccttatgcca tcgaaaaagc
      781 tcggttcgcc agtggtcttt gagcgtctcc caaccttgat tgcctcgggc gaggcaccac
      841 aggcgttcaa cgagcgatgg aaattcccgt tcgtgccaga actcaagcgc cgtcagatta
      901 tttgccgggc gacgcaactg taaatcgtcg gaatgcgaga atttgctgtg acaaaacacc
      961 accggcgtgt cacatccggc ggctggaagg gcagcgacga ctgcggccag atcgccctga
     1021 caggtgtttt catagccatc tgaaagcacc actaccaggt ctggatggtc agccacggcg
     1081 tccagcacgg ctgttgccag atcggttggg ccgttgggaa tgggcgggtc tccctggctg
     1141 ccaaccgtaa tggttcgagt tt
//
LOCUS       DAOQDO010002381         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_161824_length_1161_cov_2.381555, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002381 DAOQDO010000000
VERSION     DAOQDO010002381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161824_length_1161_cov_2.381555"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1161)
                     /locus_tag="PKZ53_27405"
     CDS             complement(<1..>1161)
                     /locus_tag="PKZ53_27405"
                     /inference="COORDINATES: protein motif:HMM:NF026036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ44242.1"
                     /translation="RAPEPFFDKPTKIFQIALTKGLRSNFKPADEVSAAVESVKDEKK
                     TDDAAKKDGEKKSTPVMIDLEGIQDRVYELPLSAGNYGGLSASDKFLFFLDYDASPDP
                     KRRLQAVEIKTRDVEAKTVSNDVNYFELSQDGKKLLVWLDSGMFVFDAGSEAPKDLGK
                     VKIPVESLVVHLDPREEWRQMFVDSWRLMRDYFYDPGMHKVDWPGMLKKYQPLVARVT
                     DRSELSDLMFEMVGELSTLHTFVRDGDPRQSPDQIAGGSLGAILERDEKGGGFRIAHI
                     FQGEPDYLEKYSPLKRPDLTINEGDIIESVNGVATLSVPHIGALLRNQAGKDVLLRVK
                     AASTGKSFDAVVKPISQNAEANLRYDEWEYRRRKMVDELSQGQIGYIHLRAMG"
BASE COUNT          261 a          319 c          289 g          292 t
ORIGIN      
        1 gcccatggcc cgcagatgga tatagccaat ttgcccctgg ctcagttcgt ccaccatctt
       61 gcggcggcgg tattcccatt cgtcatagcg gagattggct tcggcgttct gggaaattgg
      121 cttcaccacg gcatcaaacg atttgccagt gctggcggct ttgacccgca gcagcacatc
      181 tttgccggcc tgattgcgaa gcagcgcccc aatgtgcggc acagacagtg tggccacacc
      241 gttgactgac tcaataatgt cgccttcgtt gatcgtcaaa tccggacgct ttaacggcga
      301 atatttttcc aggtaatcgg gttcgccctg gaaaatatgg gcaatccgga agccaccgcc
      361 tttttcatca cgttccagga tggcaccaag cgacccgcca gcgatttgat cgggtgactg
      421 gcgcggatcc ccgtcacgga caaaggtgtg gagggttgat agttcaccaa ccatttcaaa
      481 catcaggtcg ctcagttcag atcggtcagt tacacgagca accagcggct ggtatttttt
      541 gagcattccc ggccagtcaa ctttgtgcat accggggtca taaaaatagt cacgcatcaa
      601 ccgccagcta tccacaaaca tctgccgcca ttcctcgcgt ggatcgagat gaaccacgag
      661 cgattccact ggaattttca ctttgcccag gtctttgggt gcttctgaac cggcatcaaa
      721 gacaaacatg ccggaatcaa gccagacaag cagctttttc ccgtcctgcg aaagctcgaa
      781 atagttcacg tcattggaga cggttttcgc ctcgacgtca cgggttttga tttccacggc
      841 ctgaagtctg cgtttcgggt ccggcgaagc atcgtagtcg aggaaaaaca ggaatttgtc
      901 ggaagctgac agcccgccat aattccccgc tgacaatgga agttcataca cgcgatcttg
      961 aatgccttcg agatcaatca tcactggtgt cgattttttc tcaccatctt ttttcgcggc
     1021 atcgtcagtt ttcttttcat ctttgactga ttcaacagcg gctgacactt cgtcggctgg
     1081 cttaaagttg gaccgcagcc ctttggtcag cgcaatttga aaaattttag tgggtttatc
     1141 gaaaaacggt tccggcgccc g
//
LOCUS       DAOQDO010002382         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_162301_length_1159_cov_2.946558, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002382 DAOQDO010000000
VERSION     DAOQDO010002382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162301_length_1159_cov_2.946558"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKZ53_27410"
     CDS             <1..516
                     /locus_tag="PKZ53_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNJ44243.1"
                     /translation="IFQDPSLEEMATTYPTSQEDLAAVNGVGMGKVKKFGKEFLELIQ
                     KYVDDNDIETATEVVVKSSVNKSKIKIFIIQQIDRKVDLEEIAESKSLTFDDLLTEIE
                     NICYSGTKLNLDYYIDEVLDEHKQNQIFDYFLNSETDSLEEAMADAGNSEYNEEEMRL
                     MRIKFLSEYAN"
     gene            571..>1159
                     /locus_tag="PKZ53_27415"
     CDS             571..>1159
                     /locus_tag="PKZ53_27415"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNJ44244.1"
                     /translation="MLGSGGREHALAWKIRQSPLTKSLYIAPGNAGTATLGTNVSVQA
                     EDFDGVAHICRQKQIDLVVVGPELPLVRGLRDFLASKTDLSHVLMVGPGESGARLEGS
                     KDFSKQFMLRNGIHTAKARTFTETEIEAGLSYLEGCRMPIVLKADGLAAGKGVIIAPN
                     LSEAKTALRDMLVNHLFGEASARVLVEEYLSGIEVS"
BASE COUNT          320 a          277 c          303 g          259 t
ORIGIN      
        1 attttccagg atccgtcatt ggaagaaatg gcaaccacct acccaacctc ccaggaagac
       61 ctggcggcgg tcaatggggt gggcatgggc aaggtgaaga aattcggaaa agagtttctc
      121 gaactgattc agaaatatgt tgacgacaac gatatcgaga cagccacgga agttgttgtt
      181 aagtcgtcgg tgaacaaatc aaagatcaaa atttttatta ttcagcagat cgatcgaaaa
      241 gtggacctgg aagagattgc cgaatcaaag agtctcacgt tcgatgacct gctgacggaa
      301 attgaaaaca tttgctattc cggcaccaaa ctcaacctgg attattacat cgacgaagta
      361 ttggacgaac acaagcaaaa ccagattttc gattacttcc tcaattccga aaccgatagc
      421 ctcgaggaag ccatggctga cgccgggaat tccgaatata atgaggagga aatgcgattg
      481 atgagaatca aattcctgag cgaatacgcc aactgaccgt cgcaagtcaa ggtttcttta
      541 tctttaggtc ttttacccat gaacatcctt ttgctcggta gcggcggccg cgaacacgct
      601 ctggcctgga aaatcaggca aagcccactt accaaatcgc tatacattgc tccgggtaat
      661 gccggtacgg caaccctcgg taccaatgtg agcgtacagg ccgaagactt cgatggtgtg
      721 gcgcatatct gccggcaaaa acaaattgac ctggttgtcg ttggtccgga actgccgctg
      781 gtcagggggc tgcgtgattt tctggcctca aagaccgatc tgtcacacgt gctgatggtc
      841 ggacccggag aatcgggtgc ccgacttgaa gggagcaaag acttctccaa gcagttcatg
      901 ctgcgtaatg gaattcatac tgccaaagcc agaacattca cggaaaccga aattgaagca
      961 gggttgagct acctggaagg ctgtcgaatg cccattgtgt taaaagcaga tgggcttgcc
     1021 gccgggaagg gcgtgatcat tgcccccaat ctttctgagg cgaaaacggc actgcgcgac
     1081 atgctggtca accacctctt tggagaagcc agtgcccgcg tcctggtgga agagtacctc
     1141 tccggaatag aggtttctg
//
LOCUS       DAOQDO010002383         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_162332_length_1159_cov_2.602355, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002383 DAOQDO010000000
VERSION     DAOQDO010002383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162332_length_1159_cov_2.602355"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PKZ53_27420"
     CDS             <1..1040
                     /locus_tag="PKZ53_27420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44245.1"
                     /translation="RFLPVQQGPGLHAEDRMNSQEGNTGISQSEEFQLQVNWRIQGSL
                     LIVLAIFCLGVGGFAFFKAWEHSSTGTQVTDGWTWRNALLLNAIFLCTVGAVIWETYW
                     LANLSIDAWEITKPAFFGGTHALPWSELTSLQSSSKDVVNLSFGQRKLRIAPHVFQEP
                     EKVKSAIYHFANQGLLTVEQFHPLIPRPTFQIYRFSELTRRWLVIGGISSFGLSIVSG
                     IMLAGYRRPLPPNAAYVLGFAVLSFSLFLILPKLGQLTRCSFQIDQTQITKQTGLNNR
                     FSIQWQEIELIETGPEVKHLLLKSKQTQDVMVIEPQTENFKVLKNIIQYRTNQLAPQT
                     QPPKKKKRPRR"
BASE COUNT          315 a          304 c          248 g          292 t
ORIGIN      
        1 ggcgtttcct cccggtccag caaggaccgg gtctccacgc cgaagacagg atgaacagtc
       61 aggaaggaaa taccggaata tctcaaagcg aggaatttca actacaggtg aactggcgaa
      121 tccagggatc acttctgatt gtacttgcaa ttttctgtct cggagttggc ggatttgcat
      181 tcttcaaagc ctgggaacac tcatccaccg gcacacaggt cacagatggt tggacctgga
      241 gaaatgccct tttgctcaat gcaatttttt tgtgtaccgt gggggctgtc atttgggaaa
      301 catactggct tgccaacctg agcattgatg cctgggaaat tacgaaacca gccttttttg
      361 gaggaacaca cgctctcccc tggtctgaac tgaccagcct ccaaagttct tcaaaagatg
      421 tggtcaacct ctcttttggt caaagaaaac tacggattgc cccgcatgtc tttcaagaac
      481 cggaaaaggt caaaagtgca atctatcatt ttgccaatca agggcttctg accgttgaac
      541 aatttcaccc tctcattccc agacccacct ttcaaattta ccgattttcc gaactcaccc
      601 ggcgttggct ggttattggc ggaataagta gctttggact ctctattgta tctggaatta
      661 tgctggcagg gtaccgacga ccactgcctc ccaatgcggc atacgttctc ggttttgcag
      721 tcctttcatt ttccctgttc ctgattctcc ccaaattagg gcaactgaca cgctgttcat
      781 ttcagattga ccagacccaa atcaccaaac aaacaggact caacaaccga ttcagcatcc
      841 agtggcagga gattgaactg attgagactg ggcctgaagt gaaacatctt cttttaaaat
      901 caaaacagac ccaggatgtg atggtcattg agccgcaaac tgagaatttc aaggttttaa
      961 aaaacatcat ccagtatcga accaaccaac tggcacccca gactcaacct cccaaaaaga
     1021 aaaagcggcc tcgaagataa cgtcatctcc tgaacccgct tttgcgtcca aaccctacag
     1081 ccccatctga agacgctttc acatgttggt tttgattacg cttcagcaag acaagacacg
     1141 tggtgactcc gttgcggaa
//
LOCUS       DAOQDO010002384         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_162351_length_1159_cov_2.430254, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002384 DAOQDO010000000
VERSION     DAOQDO010002384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162351_length_1159_cov_2.430254"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKZ53_27425"
     CDS             <1..652
                     /locus_tag="PKZ53_27425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ44246.1"
                     /translation="EINKRGTIDCSTDSLLFHNHEEPHSSVKAGGISRHFQVELSLDW
                     CRKFEIDPDRMPGSSKLLNPKIKLCFYNIYQESSVIDETSNLTIDALLVQAFETMQGA
                     DHRQPSTRPKWVTRIDEILHDQFDQPLTLSDLSTELDLHVAHLSRDFPRYFRCNFSQY
                     IRMIKVEKALSMLRNRKVPLTEIAVNCGFADQSHFIRCFKEFVGVTPKAYRKRVHS"
     gene            844..>1159
                     /locus_tag="PKZ53_27430"
     CDS             844..>1159
                     /locus_tag="PKZ53_27430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44247.1"
                     /translation="MKMYRPRKIQLNRRLAINNWNVKVYTITHQSRFESAVTLQSAIA
                     RLPGWLEKSRATGFETHGVAFLIVHEGLDGVWTLINWWMCDELLQSLTFFTSYKTPTE
                     FQP"
BASE COUNT          316 a          291 c          264 g          288 t
ORIGIN      
        1 cgagatcaat aaacgaggga cgattgactg ttcaaccgat tcactgctat ttcacaacca
       61 tgaggagccc cattccagcg tcaaagcggg tggtatttca cgtcattttc aggttgaact
      121 cagcctcgac tggtgtcgga agtttgagat tgatccagac cggatgcccg gaagctcaaa
      181 actcctgaac ccaaagatta agttgtgttt ctacaatatt taccaggaat caagcgttat
      241 tgacgagaca tcaaacctga cgattgatgc cctgctggta caggcgtttg aaacgatgca
      301 gggtgccgac catcggcagc cttcaacccg accgaaatgg gtcacccgga ttgacgagat
      361 tttgcacgat cagtttgacc aaccgctcac cctcagtgac ttatcaactg aattagacct
      421 ccacgtagcg cacctttcac gtgattttcc aaggtacttt cgatgcaatt tcagccaata
      481 tatccgaatg atcaaggtcg aaaaggcgct ttcgatgctg cgcaatcgaa aggtcccgct
      541 gacggaaatt gctgtaaact gtgggtttgc agatcagagt cattttatcc gttgctttaa
      601 agaatttgtt ggagttacgc caaaagcgta tcgtaaaaga gtgcattcat aaccgatctg
      661 tgccacataa agccgaagac tgaaaaattc ccgcccacgt taaacctgtt ctattttctc
      721 attgctcagt ccttctactc tttggaatct gaccccaaac ccactggctt ggctcgacga
      781 tcagacggcc cagccagacg tttgttgatc aacattccag atctgcaaag gcactgagac
      841 accatgaaaa tgtatcgtcc ccgcaaaata cagttgaacc ggcgcttagc cataaataac
      901 tggaacgtca aagtttacac cattacgcat caaagccggt ttgaatcagc cgtaacattg
      961 caaagtgcaa tcgccaggct tccagggtgg ctggaaaaat ctcgggcaac aggctttgag
     1021 acgcacggtg tcgcgttcct gattgtgcac gagggcctgg acggggtgtg gactttaatc
     1081 aactggtgga tgtgtgacga gctgttacaa agcctgacgt tctttaccag ctataaaact
     1141 ccgaccgaat ttcaaccct
//
LOCUS       DAOQDO010002385         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_162574_length_1158_cov_3.352675, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002385 DAOQDO010000000
VERSION     DAOQDO010002385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162574_length_1158_cov_3.352675"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1158)
                     /locus_tag="PKZ53_27435"
     CDS             complement(<1..>1158)
                     /locus_tag="PKZ53_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ44248.1"
                     /translation="DAGKTWNKLSSGLPTEQMGRIGLAIAPTDPQMVYAQIESTNKAG
                     GIFRSTDSGATWEKRNPYDAQAQYYAHIYVDPKNAERIYIMGVLIKVSDDGGKTLKNL
                     GEKNKHVDNHVIWIDPNDTSYYLVGCDGGVYESFDRAANWNYKSNLSITQFYDVTVDN
                     AKPFYNIYGGTQDNFSLGGPSRTRSASGIVNSDWFVTQGGDGFRSQVDPVDPNIVYSE
                     YQYGGLTRYDKRTGQRTGIKPVEGKGEEAYRWNWDSPLIISPHLNTRLYFAANKLFRS
                     DDRGNTWKAVSGDLTRRIDRNSLPVMGRVWEADAVAKNVSTSLYGNITALAESPKKEG
                     LLFVGTDDGLIQISEDGGANWRKLEKFPEVPENTYVTRLLASQHDAATVYAT"
BASE COUNT          244 a          296 c          318 g          300 t
ORIGIN      
        1 gtggcgtaca ccgtggcggc atcatgctga gaagccagca ggcgggttac ataggtgttt
       61 tcagggactt cggggaattt ctccagcttg cgccagttag ccccaccatc ttcgctgatt
      121 tggatcaagc catcatctgt tccgacaaac agcagacctt cttttttcgg agattcggcc
      181 agtgcggtaa tgttgccata gagtgaagtc gagacatttt tggcgaccgc atcagcttcc
      241 catacgcgcc ccataaccgg cagggaattg cggtcaatcc ggcgcgtcag atcaccactg
      301 acggctttcc aggtattgcc acggtcgtcg ctccggaaca gtttgttggc agcaaaatag
      361 agtcgggtat tgaggtgtgg gctaatgatt aatggtgagt cccagttcca gcgataggct
      421 tcttcgcctt tgccttcaac tggtttgatt ccagtgcgtt ggcccgttcg tttgtcataa
      481 cgggtcagcc cgccgtactg gtattcggag tagacaatgt tggggtcaac tgggtcaacc
      541 tgtgaccgga acccgtcgcc gccctgggtg acaaaccagt cggaattgac gatccccgaa
      601 gcacttcggg tgcgtgacgg cccgcccaga ctgaaattat cctgggtgcc gccatagata
      661 ttgtaaaaag gtttggcgtt atcaaccgtc acatcataaa actgggtgat cgagaggttt
      721 gacttgtagt tccagtttgc cgcccgatca aaactttcat aaaccccacc atcgcaacca
      781 accaggtaat agctggtgtc gttggggtca atccagatca catgattgtc aacgtgttta
      841 tttttctcgc ccagattctt gagcgtcttg ccaccatcgt cagaaacttt gatcagcacg
      901 cccataatgt aaatgcgctc ggcatttttg ggatcaacat aaatgtgggc atagtactga
      961 gcctgggcgt cataggggtt acgtttttcc caggtggccc cggaatctgt tgagcggaag
     1021 atccctccgg ctttgttggt ggattcaatc tgggcataga ccatctgggg atcggttggg
     1081 gcaatggcca gcccgatgcg gcccatttgt tcggttggca accctgacga gagtttgttc
     1141 caggtcttgc cggcgtcg
//
LOCUS       DAOQDO010002386         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_163413_length_1155_cov_3.021818, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002386 DAOQDO010000000
VERSION     DAOQDO010002386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163413_length_1155_cov_3.021818"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKZ53_27440"
     CDS             <1..895
                     /locus_tag="PKZ53_27440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ44249.1"
                     /translation="ISKTPLTLLDRLRELRVLIVDDNRVNLTILQQQLQVWEMTLSTA
                     TTGEQALTLLHRAAQEGKPYNLALLDVQMPDIDGFTVAQIIHSDPILSSTHLILTPSF
                     HNPAQVELARQLGIAAILVKPIRQNQLLDCLKTLFPPPTIDEPSGTPSTRLPFPGGRT
                     VHAQILVAEDNPINQRFLVRQLERMGYRANVAGNGYEVLASLKLIPYSLILMDCQMPE
                     MDGFETATAIRQLEKEPQSTPAFAYQLRSVKQIPIIGMVGDTKPSTREKCRESGMDSY
                     LTKPIRYEDLLEAINCWLPFE"
BASE COUNT          265 a          345 c          284 g          261 t
ORIGIN      
        1 gatttcaaag accccgctga ccttgcttga tcgcctccgg gagctgcgcg tcctgatcgt
       61 ggacgataat cgggtgaact tgacgatttt gcagcagcag ctccaggtct gggaaatgac
      121 gctctcgacc gccaccactg gggaacaggc attgaccctc ctgcaccggg cagcccagga
      181 aggcaaacct tacaacctgg cgttacttga tgttcaaatg cctgatattg acggatttac
      241 cgttgcccaa atcattcatt ccgacccgat cctgtcatcc acccatctga ttctgacccc
      301 ttcgtttcac aaccccgccc aggttgaact ggcccgtcaa ctgggaatcg cagccatcct
      361 ggtcaaacca atccgccaga atcaattgct cgattgcctc aaaaccctgt ttccaccacc
      421 aaccattgac gaaccttccg ggactccctc gacccgattg ccattcccag gtggacgaac
      481 ggtccatgcc caaattctgg tcgcggaaga caacccgatc aaccaacggt ttctggttcg
      541 tcagcttgaa cggatgggct atcgggccaa tgtggcggga aatgggtatg aagtactggc
      601 ctcactcaag ctcattccct attcgctcat tttgatggac tgccagatgc cggaaatgga
      661 tggctttgaa accgcaaccg cgattcgtca acttgaaaaa gaacctcaat caacacctgc
      721 ctttgcctac cagctccgct ccgtcaaaca gattccaatt attgggatgg ttggcgacac
      781 caaaccttcg acccgcgaaa aatgccgcga atcgggtatg gattcctacc tgacaaaacc
      841 aattcgctat gaagacctgc tcgaagccat caattgctgg ctgccgtttg agtgagtagc
      901 gcggagtaag gagcgatgtc tttaccgaag gggaagaagg cccttcggcc cgtacacttt
      961 cggagtgcgg cccacttgac gtcgctttgc atcccgtaga caaaccgcgt aataccaggg
     1021 ttcagggttc agggtttgct gatggccggt tggtcgcggt gctgatgatc tttttgatgc
     1081 ggcggccttt tttcggcgtc atccgaaagc ggtgtcaagc caccgcactc cacaggcgtc
     1141 aagccgccca aatcc
//
LOCUS       DAOQDO010002387         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_163448_length_1155_cov_2.512727, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002387 DAOQDO010000000
VERSION     DAOQDO010002387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163448_length_1155_cov_2.512727"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PKZ53_27445"
     CDS             complement(<1..939)
                     /locus_tag="PKZ53_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ44250.1"
                     /translation="MYQVADAELSGPFQKLAPDATAKAGAFVFLDSKEGTSSQDQYTQ
                     VSTALSQGAKAVLLVIGEREQAMWERFSKQSPELPPGNAQAPTIIFVEKSAAETLRQV
                     ADGTLLSIKGKVQAGEVKQTWNVVGCLPGSDPKQKAEVILLSAHMDHIGGKYSPDKDT
                     IYNGADDDASGVAAVLELARALGNSPRSKRSVYFVCFGSEEIGGPGARYFLEHPPVPL
                     KNIVANLEFEMIGRPDPKVGPETLWLTGFDLSDLGAELAKQGAHIVMDPHPQEFFFQR
                     SDNYALAKRGVIAHTVSSYGLHADYHKVSDEIEKIDF"
BASE COUNT          261 a          305 c          293 g          296 t
ORIGIN      
        1 aaaatcaatt ttctcaatct cgtcgcttac cttgtggtag tcagcatgca atccatagct
       61 tgaaaccgtg tgcgcaatga caccccgttt ggccagtgca taattgtcag atcgttgaaa
      121 gaaaaattcc tgtgggtggg gatccatcac aatgtgggcc ccctgtttgg ccagttccgc
      181 ccccaggtca gacagatcaa agccagtcaa ccatagtgtt tccggtccaa ctttcgggtc
      241 aggtcgcccg atcatttcaa attcgaggtt ggcgacaata tttttgagcg gaacgggtgg
      301 atgctctaag aaatatcggg cgccaggtcc gccaatttct tcgctgccaa aacagacaaa
      361 atagacgctt cgctttgagc ggggactgtt tcctaaagct cgggccagtt ctaaaactgc
      421 cgccacacct gacgcgtcgt catcggcccc attgtaaatc gtgtctttat ccggagagta
      481 cttgcccccg atgtgatcca tatgggctga caggagaata acttcagctt tctgtttggg
      541 atcacttcca ggcaggcatc caacgacgtt ccaggtttgt ttgacttcac cagcctgcac
      601 ttttcctttg attgaaagca gggttccgtc agcaacctgg cgcaatgttt cagccgctga
      661 tttttcaacg aaaatgatgg ttggcgcctg ggcattgccc ggagggagtt ccggcgattg
      721 tttcgaaaag cgttcccaca tggcctgttc gcgttcacca atcacaagca acacggcttt
      781 tgccccttga gacagtgcgg ttgaaacctg tgtgtattga tcctgggaac tggtgccctc
      841 cttgctgtcc agaaagacaa aggctccggc tttggcagtc gcatcaggcg ctaatttttg
      901 aaacggaccg ctgagttcgg cgtccgccac ctgatacatt acaacttcct tgccatgggt
      961 ccaggtcact ggagttgaag ccccaggaaa tgacaactgt ggcggttcag caaaggtgcg
     1021 tttggtcagc gttgccgttt gaaaataggt cacattcccg gacttatctt tttccccggc
     1081 aggttcaatc ccgaactgac gaaactgagc agcaatgtac tgaccggcga tgcgttcaaa
     1141 ctgcgtggca ctggc
//
LOCUS       DAOQDO010002388         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_163562_length_1155_cov_1.779091, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002388 DAOQDO010000000
VERSION     DAOQDO010002388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163562_length_1155_cov_1.779091"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKZ53_27450"
     CDS             complement(<1..321)
                     /locus_tag="PKZ53_27450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNJ44251.1"
                     /translation="MKNRLLLALLVTSLLSGCTLGPKYIRPEVAPPPVFRGDTMPATP
                     TNDATSLADLKWFEMFKDDQLQGLIRTALVENYDLRDAVARVDAAQANLGITRADQIP
                     NLAVG"
     gene            complement(345..>1155)
                     /locus_tag="PKZ53_27455"
     CDS             complement(345..>1155)
                     /locus_tag="PKZ53_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ44252.1"
                     /translation="IRNYYVRNKEGQMVPLSALVTIRRTSGPEFTNRFNLYRSAQILG
                     GTAPGYSSGQALTALEEVARETLPPEMGYEWADLSYQEKKASGAAGVAFGLSLVFVFL
                     ILAALYESWSLPFSVLLTVPIAVLGAFGGLLLRGYDLGVYAQIGLIVLIGLAAKNAIL
                     IVEFAKLELEKGRDLVDAALEGARLRLRPILMTSFAFILGCFPLWAATGSGAAGRRIL
                     GTVVIVGMLAATVIAIFFIPVTFYVVERLTGNHKEPEVESFPMEPAPETGD"
BASE COUNT          288 a          347 c          285 g          235 t
ORIGIN      
        1 gccaaccgca aggttcggaa tctgatcggc cctggtgatc ccaagattgg cctgtgctgc
       61 atccacccgc gccacggcat cccgaaggtc gtaattttca accagtgccg tccggatcag
      121 cccttgcaac tgatcatctt taaacatttc gaaccacttg aggtcagcca gcgaagtcgc
      181 gtcattggtc ggggttgccg gcatggtgtc tccccgaaaa actggaggcg gagcaacttc
      241 aggccggatg tatttgggac ccagcgtaca cccagataac aggctggtca caagcaaggc
      301 aagcaacaat cggtttttca caagtttgat cctttcctgg tctctcaatc tccagtttca
      361 ggggcaggtt ccatcggaaa gctttccact tcaggttctt tgtgatttcc ggtcaatcgc
      421 tccacaacat aaaaagtcac tggaatgaaa aagatggcaa tcactgtggc ggcaagcatt
      481 ccgacaatga ccaccgtgcc gaggattcgc cgtccagcgg cccctgaacc agtcgctgcc
      541 cacaacggga aacaacccaa aatgaaggca aacgatgtca tcaaaatggg tcgcagccga
      601 agtcgagcgc cttcaagcgc ggcatcaacc agatcacgac ctttttccag ttccagtttg
      661 gcaaattcca caatcagaat ggcgttttta gcggccagtc cgatgagcac aatcagcccg
      721 atttgggcat acacgcccag atcatagcca cgcagcaaca gccctccgaa cgcccctaaa
      781 accgcaatcg gcacggtcag caacactgaa aacggcaacg accagctttc atacagcgcc
      841 gccagaatca gaaacacaaa gacgagcgac aggccaaagg ccactccggc agcgcccgac
      901 gccttctttt cctggtatga aaggtcggcc cattcatagc ccatttccgg cggcagcgtt
      961 tccctggcaa cttcttcgag tgccgtcagt gcctgacccg aactgtatcc gggcgcggtt
     1021 ccgcccagga tttgcgccga acggtagaga ttaaaccggt tggtaaattc cgggccactc
     1081 gttcgacgga tggtgaccag tgccgaaagc ggcaccatct gcccctcttt attgcggaca
     1141 taataatttc ggatg
//
LOCUS       DAOQDO010002389         1154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_163693_length_1154_cov_3.151956, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002389 DAOQDO010000000
VERSION     DAOQDO010002389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163693_length_1154_cov_3.151956"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKZ53_27460"
     CDS             complement(<1..863)
                     /locus_tag="PKZ53_27460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44253.1"
                     /translation="MFILAVATIAFTRFHPPETVLSANAWGAGVPNGSCITAFATFKP
                     WWIRHTPNPVVGTASGGIEPLVKIPRDTVAVKFRYDDQGNQSPGKILPERGIGYEAIL
                     GGLTALRSLFSTDLIFDFALFIRLQQWSLVGALILVPLGFYGVGSSPTIRQSMLVFTL
                     VYLTLLILNPNINEYLNEGIIDSSLANTAAVLCCSAWFFILKFAELKSFKEILGVSAL
                     SLVLSFSTHIRGEFLIVLMAVFAFNFCILFKHNKLKLRGLLLALVILFSLPVGYGLIN
                     KAIFGHFIPLR"
BASE COUNT          322 a          279 c          300 g          253 t
ORIGIN      
        1 attctaagtg gaatgaagtg gccaaaaatc gctttgttga tcagtccata gccaaccggc
       61 agtgaaaaga gaatcaccag cgccagcaaa agacctctca gttttaattt gttgtgtttg
      121 aacaagatac agaaattaaa ggcgaacaca gccatcaaga cgatcagaaa ttcaccccgg
      181 atatgagtcg aaaagctcag cacgaggctc aaggcactga cacccagaat ttctttgaag
      241 gactttaatt cggcaaactt cagaatgaaa aaccaggcac tgcaacacaa cacggcagcc
      301 gtattggcca gggaactatc aataatgcct tcattgaggt actcgtttat gttggggttc
      361 aagatgagca gcgtcagata gaccagcgtg aacaccagca tggattggcg gatagttgga
      421 gatgagccca caccgtaaaa tcccagcggc accaggatca acgctccgac cagcgaccac
      481 tgctgcaggc gaataaataa agcaaaatca aaaatcaggt cggtagaaaa aaggctccgc
      541 agggcggtga gccctcccaa aatggcttcg tagccaatcc cgcgttcggg aaggattttt
      601 cccggcgatt gatttccctg atcgtcatat cggaatttta cggcaactgt atcgcgtgga
      661 attttgacca gtggttcaat tcctccagaa gcggttccta cgaccgggtt aggtgtgtgc
      721 cgaatccacc agggcttgaa cgtagcaaag gccgtgatac agcttccatt gggaaccccg
      781 gcgccccagg cgttggctga aagcacggtt tcaggtgggt gaaacctggt gaaggcaatg
      841 gtggcgacgg caagaatgaa caaacaaagg cctggtaaat accgtgtcat aaatgagatg
      901 agagctgggt ggtgatctat ttttcttttt ggctgaacaa tgtgctggct catctcagtt
      961 gcggcaggga agatggcctg cgttatcgta gcacccagcg aactgatccg tctttcatca
     1021 ataccacaat cacctgtttc cagtgttgaa gctggtctga aggtatttct tcaaaccgga
     1081 acttcccatc cgccccagcc tgaactggcc gggtccagta acagtctggc ggcggggaac
     1141 ttcgagcgac cgtg
//
LOCUS       DAOQDO010002390         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_164394_length_1152_cov_1.865998, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002390 DAOQDO010000000
VERSION     DAOQDO010002390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164394_length_1152_cov_1.865998"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1071
                     /locus_tag="PKZ53_27465"
     CDS             166..1071
                     /locus_tag="PKZ53_27465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44254.1"
                     /translation="MLRKLCGFCLGMVLLSSMACAPARTPNSNSETSKPASSGGVSLT
                     YKPPMVPISFTVDTNGNISSQLDQSLVTPLGQFTLGGFVEQNWSSNSPKLKVIFRNSS
                     QNLERVYELQETDKPLKISTDHKTDILLQPGFVIIEVGNVEALKVNFTPADRSGGSGQ
                     VMLAESTAPSNQVQPVYVQLEKVEVYEDGSAGATDWGFDISAGERSLTSIPRQSFHDD
                     KERQVMVFNPDETTKLLQVKGDSLKVKVMGRNYDKDCFALGEAQISLDQLDLQSKSIQ
                     LFAVVPGNSKRGFFKFSFVVKPAEV"
BASE COUNT          306 a          277 c          277 g          292 t
ORIGIN      
        1 gctcaactca gccaaaccca aacaaaaaac cgacacgcga actggattgc acctgggcta
       61 tgagctgtcc acccgattgg cgagttcaaa cccgatcttt tttcgcatta ttgagtcaca
      121 cctgaaccct gtacaagtgt tatggatacc cggaggttta tttttatgct tcgaaaacta
      181 tgtggtttct gcctcggaat ggtgctgctt tcatcaatgg cctgtgctcc ggcacgaacg
      241 ccaaattcca actctgagac ttcaaagcca gcttcaagcg gaggcgtctc attaacctat
      301 aaaccgccca tggtcccgat cagttttacc gtggatacca acgggaatat ttcatcccag
      361 cttgaccaaa gccttgtcac gcccttgggt cagtttacct tgggaggctt tgtggaacag
      421 aactggagtt ccaattctcc aaaattgaaa gtgatctttc gcaattcaag ccagaatctg
      481 gaacgagtct atgagttaca ggaaaccgat aagcctttga aaatttcgac ggaccacaaa
      541 acagatattc tgcttcagcc gggatttgtc atcattgaag tcggcaatgt tgaagcgctc
      601 aaggtaaatt ttactccggc ggaccgaagc gggggttccg ggcaagtcat gctggccgaa
      661 tcaaccgctc cttccaacca ggtccaaccg gtctatgtcc aactggaaaa agtcgaagtc
      721 tatgaagatg ggtcggcagg tgcgacggat tggggctttg atatttcagc cggagagcgg
      781 agtttaacca gcattccgcg ccaatcgttc catgacgaca aagaacgtca ggtgatggtt
      841 tttaaccccg atgagacgac aaaattacta caggtcaaag gtgactcgct caaagtcaaa
      901 gtgatggggc gtaattacga taaagattgt tttgccctgg gcgaagcaca aatttcactt
      961 gaccagttgg atctgcaatc caagtctatt cagctttttg cggttgtccc aggaaattcc
     1021 aaacgcgggt tctttaagtt ttcattcgtg gtcaaacccg ctgaagtcta gtgcaattga
     1081 cgggtcagca gagtggagtc aggggtcagg gaaatatcat tttcccaact ccaataccat
     1141 tttgggctga gg
//
LOCUS       DAOQDO010002391         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_164661_length_1151_cov_1.953467, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002391 DAOQDO010000000
VERSION     DAOQDO010002391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164661_length_1151_cov_1.953467"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKZ53_27470"
     CDS             complement(<1..559)
                     /locus_tag="PKZ53_27470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44255.1"
                     /translation="MSEKKNRTHITRIGLTLGMTLLLGWLALISAPVSRLETRQTPAR
                     RGIQKGTSAKLTPGQAQMAQGVASPETRGRFTPNASISVEPVSLAVPPFFDSVTGAHF
                     LQKNAMSFDGRFIVYRSSASNLAVGQIDSNTANDIFVFDRQTGTAELVSRVAGTSATA
                     GNSISDTPVISANGRYVAYQSFSTNL"
BASE COUNT          273 a          287 c          275 g          316 t
ORIGIN      
        1 ccagattggt ggaaaagctc tgataggcaa catatctccc attggcactg atgaccggag
       61 tatctgaaat gctatttccc gccgttgcgc tcgtcccggc tacccggctg acgagttcgg
      121 cagttccggt ttgccgatca aagacgaaaa tatcattggc tgtattggaa tcaatctggc
      181 ccactgccag gttggaagcc gaactccgat aaacgataaa ccgcccatca aaactcatcg
      241 cgtttttttg gagaaagtgt gccccggtga ctgaatcaaa gaatggaggc accgccagcg
      301 agacgggttc gaccgatatg ctggcattgg gggtaaacct ccctcgtgtt tcaggcgaag
      361 ccactccctg ggccatctgg gcctgacctg gcgtgagttt ggcagacgtt cctttttgga
      421 ttccacgtct ggcaggcgtt tgcctggttt ctaatcggga aactggggca ctgatcaagg
      481 ccagccagcc aagcagcagg gtcattccca gggttaaccc gattcgggtg atgtgggttc
      541 ggtttttttt ctctgacata aaatcctctc attcaatcgc caggtcctca cagcgttcct
      601 tttggtgagg caccatctgt gtttgacgaa tacatcattt gtaaataatt caagcttctt
      661 aaggttatta agctcagtaa tttcagcgat ttatagcctt acaaacagaa agcacacctg
      721 tcctgcgtgt gttacatcaa ctttagttca ggtcgtttag ataattattt ccggcgaggt
      781 ttctttttct gaaaaacgct gtctcctttt aaaagtcgtg aaggaggttg gaactgcctt
      841 catgacctta agcaggaaat caactcaaac catgtcatct ggtttgaaaa atttttggtc
      901 tcctcgccag attgggttaa accaaaaaaa ttcgggctga agatgttggg ttcactgatc
      961 atgaatcggc atatcttcag ccgattatgt ctttcatcgc aatcgggttt actggatctt
     1021 tgatggcgcc cggaactgtc caacaccagt tccaatattg tttggagcac ccaccagcca
     1081 ccagccgccg gttggaagta cccttccctg gatgcgattg ttcccggtat ctcccaccac
     1141 cagaaccagg t
//
LOCUS       DAOQDO010002392         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165148_length_1149_cov_2.478976, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002392 DAOQDO010000000
VERSION     DAOQDO010002392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165148_length_1149_cov_2.478976"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKZ53_27475"
     CDS             <1..341
                     /locus_tag="PKZ53_27475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ44256.1"
                     /translation="ISNNWGNAEGWCQDLGFDQYFELVIDSTLVNVRKPDAKIFQIAL
                     EKMNLPAEKTAYIGDQFVADVLGSKSVGMMSVWLQSNPPKPCPDASKVDWCITQIEDL
                     LELKALPRSS"
     gene            362..>1149
                     /gene="proB"
                     /locus_tag="PKZ53_27480"
     CDS             362..>1149
                     /gene="proB"
                     /locus_tag="PKZ53_27480"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNJ44257.1"
                     /translation="MRKFSLKNVKRIVIKIGSSVLVDAGAGLSEEVFASLTTQVAYLR
                     QANKEVVLVSSGAIAAGRVRLGMTAGPKTMPYKQAAAAVGQSQLMQAYERHFATHNLQ
                     AAQILLTQDDLVDRGRFLNARNTLFALLELGVVPIINENDTVVVEEIKLGDNDRLSSL
                     VTNLIDADLLVILSDIEGLFDSDPKANPDAQLISLVENLDRQTASLAAETKSAFGTGG
                     MVTKLEAARTASRFGIPTLLARGRRPHCLIDLFAGKAIGTLFLP"
BASE COUNT          286 a          284 c          296 g          283 t
ORIGIN      
        1 gtatcagcaa taactgggga aatgccgaag gctggtgtca ggatcttggt tttgatcagt
       61 attttgaact ggtgattgat tcgaccctgg tcaatgtccg caaaccggac gcaaaaatct
      121 ttcagattgc gcttgaaaag atgaacctcc cagccgaaaa aacagcctat atcggagatc
      181 aatttgtcgc cgatgtgctc ggctcaaagt cagttggaat gatgtcagtc tggctccaga
      241 gcaatcctcc caagccctgc cctgatgcgt ccaaagttga ctggtgtatt acccaaattg
      301 aggatttgct cgaattgaaa gccttgccca gatcttcttg aaacacttga acataaaaac
      361 catgcgaaaa ttctcattaa aaaatgtcaa acggattgtg attaaaatcg gcagcagcgt
      421 cctggttgat gccggggccg gtttgagtga agaggtattt gcctccttaa caactcaggt
      481 ggcttatctt cgccaggcca acaaggaggt cgttttggtg tcctccggtg cgattgcggc
      541 aggtcgagtt cggctgggaa tgaccgccgg ccccaaaacc atgccctata aacaggccgc
      601 cgctgcggtt ggtcagagcc agttgatgca ggcgtatgag cgccactttg ccacccacaa
      661 cctccaggcg gcacaaattt tgctgaccca ggacgatttg gttgaccgtg ggcgatttct
      721 caatgcccgc aacacccttt ttgcactgct cgaactcggg gtggttccga tcatcaatga
      781 aaatgacaca gtggtggtcg aagaaatcaa actcggtgat aatgatcgcc tgagttcact
      841 ggtcacgaat ttgattgatg ctgatttgct ggtgatttta tcggatatcg aagggctctt
      901 tgattccgac cccaaagcca accccgacgc acaattgatt tcactggttg aaaacctgga
      961 ccgtcaaacg gcttcactcg cggctgagac caaaagtgcg tttggaacgg gcgggatggt
     1021 caccaaactg gaagcggcca gaactgcttc aagatttgga attccaacgc tcctggcccg
     1081 tggtcgccga ccgcactgcc tgattgacct gtttgctgga aaagcgattg gaacgttgtt
     1141 tcttccaag
//
LOCUS       DAOQDO010002393         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165375_length_1148_cov_3.298262, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002393 DAOQDO010000000
VERSION     DAOQDO010002393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165375_length_1148_cov_3.298262"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKZ53_27485"
     CDS             complement(<1..202)
                     /locus_tag="PKZ53_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ44258.1"
                     /translation="MNTLATLLIVDDTPSVCHVLVDMLESPEYHIETANDGFEGLLKA
                     RALKPDLILLDVMMPGMDGFEVC"
     gene            complement(258..>1148)
                     /locus_tag="PKZ53_27490"
     CDS             complement(258..>1148)
                     /locus_tag="PKZ53_27490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ44259.1"
                     /translation="QDLVDPCVRSIQPALQARRLHCEVVHEGAATAILGDLKLLRQSF
                     LQLLDNAIKFSPPDGHFGITTRYHARKHSVHLTVWDHGPGISSSSFKFLFWPFTQIDG
                     SLARHHDGAGLGLPVTVRLVQAQGGCTILETKPGQGSRFTIVLPATTANSFSRPDYHL
                     VLDALIQNLTGLGRPVLFQSSHQALVSPILQLAEALESAAILTHTTEDSLHWLTHHQA
                     AALVIEAPLVTREKLELISQFQRVPGLGRVPLVLVNSLDIPGDAELCEMAGVTHYICN
                     PVTLPELADLLQTCLPKTNP"
BASE COUNT          299 a          305 c          310 g          234 t
ORIGIN      
        1 ggcagacttc aaagccatcc ataccgggca tcatcacatc gagcaggatc aaatcgggtt
       61 tgagcgcacg ggctttgagc agcccttcaa acccatcatt ggctgtctcg atgtgatatt
      121 caggcgattc gagcatatcc accagaacat ggcatacgct cggggtatcg tccacaatca
      181 acaacgtcgc cagtgtgttc ataactcaat ctttggaagc atcggcaaga cccaaaccgt
      241 tcttgccgga gagacaatca gggattggtt tttggcaggc aggtttgcaa caggtctgcc
      301 agttcaggaa gcgtaactgg attgcagata tagtgcgtga caccagccat ttcgcataac
      361 tcagcgtcac cggggatatc aaggctattg accagcacca gcggcacccg gcccagccca
      421 ggcacccgtt ggaactggga gatcagttca agtttttcgc gcgtcaccag cggggcctca
      481 atgaccaggg cggcagcctg gtggtgagtg agccagtgaa gtgaatcttc ggtggtgtgg
      541 gtcagaatcg cggcggactc aagggcttcg gccagttgca agatcggaga gaccagggct
      601 tgatggctgg attgaaacaa aaccggacgt ccaagaccgg tcagattctg gatcaacgca
      661 tcaaggacca ggtgatagtc aggtcttgaa aatgaattgg cggttgtcgc cggcaacaca
      721 atggtaaacc gtgacccctg tccaggtttg gtctccaaaa tcgtgcaccc accctgagcc
      781 tgaaccaggc gcaccgttac aggcagaccc aatccagcgc catcatggtg tcttgccaaa
      841 ctaccatcaa tttgggtaaa cggccagaat aaaaatttaa aactgctgct cgaaattcca
      901 ggcccgtgat cccaaacagt aagatgaaca ctgtgttttc gggcatgata gcgggtggta
      961 atcccaaagt gtccatctgg aggggaaaat ttgatggcat tgtccaacaa ctgcagaaaa
     1021 ctctgtcgca acagtttcaa atcgcccagg atagcggtgg cagccccctc atgcaccacc
     1081 tcacagtgaa gccgccgggc ctggagcgcc ggctgaattg atcgaacaca agggtcaacc
     1141 agatcctg
//
LOCUS       DAOQDO010002394         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165411_length_1148_cov_2.686185, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002394 DAOQDO010000000
VERSION     DAOQDO010002394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165411_length_1148_cov_2.686185"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKZ53_27495"
     CDS             complement(<1..514)
                     /locus_tag="PKZ53_27495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ44260.1"
                     /translation="MSCQEFEFHIALYVEGDLTAPQAQQIESHLAVCESCRAFASDLQ
                     TSQLALKTFAGGDVEDAVLDQVRHKVMTQIRLLEPVKPGFWERLAMSWGWRFALASVL
                     VALVVLPLALVVFQPKPATTVRVNSPIDHLPGTTPVSNPTPVANDSSSVPTTVAKTTV
                     TKITRRVHRPQ"
     gene            complement(511..1146)
                     /locus_tag="PKZ53_27500"
     CDS             complement(511..1146)
                     /locus_tag="PKZ53_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ44261.1"
                     /translation="MIALLSRDEARKDAQPVAQSLVARVKSGDTQAFEELFIRYQRQV
                     MRTASRLLGNVEDAGDATQEVFLRLHKYLHRFDEEREFSPWLYQVTVNVCREIATKRP
                     GHQMLSLDQEQERGALDHLASTHNQEQEFGAAQERQMIADAIASLPEKERAALVLRDL
                     EGLDTSEVARLLGSTETTIRSQISMARVKIKKFRDKWLGKTGFGQSEGRLR"
BASE COUNT          227 a          329 c          319 g          273 t
ORIGIN      
        1 gttgtgggcg atgcactcgc cgggtgatct ttgtcaccgt ggttttggca actgttgttg
       61 ggacagaact tgaatcgttg gccacgggtg ttgggtttga tacgggtgtt gtacccggaa
      121 ggtgatcaat cgggctattc actctgacgg tagttgcagg tttgggctga aacaccacca
      181 aagccagcgg caacaccacc agtgccacca gcactgacgc caatgcaaag cgccatcccc
      241 agctcatcgc cagccgctcc caaaagcctg gtttcacagg ttccagcaag cgaatctggg
      301 tcatgacttt gtggcggacc tgatccagca cggcatcttc aacgtctccg ccagcaaatg
      361 ttttgagcgc cagttggctt gtttgcaggt cgctggcaaa tgcccggcac gattcgcaaa
      421 cggccagatg tgattcaatc tgctgggctt gcggagcggt gaggtcacct tcaacataga
      481 gcgcaatgtg gaattcaaat tcctggcaac tcatcgcaac ctcccttctg attgaccgaa
      541 gccggttttc cccagccatt tatcgcggaa tttcttgatc ttgacccgtg ccatactgat
      601 ctggctgcga atcgtcgttt cagttgaccc cagcaaccgg gcgacttcgc tggtgtcgag
      661 tccttctaaa tcgcgcaaca ccagcgccgc acgctctttt tctggcagcg aagcaatggc
      721 gtcggcaatc atctggcgct cctgggcagc gccaaattcc tgttcctggt tgtgcgtact
      781 ggccagatgg tcgagtgcgc cgcgttcctg ttcctggtcc agcgagagca tctggtgtcc
      841 aggtcgtttg gtggcaattt cacggcagac attgactgtc acctgataga gccagggcga
      901 aaattcgcgt tcttcgtcaa accggtgaag gtatttgtgc aaccggagga agacttcctg
      961 ggtcgcatct ccagcatcct caacgtttcc gagcaaccga ctggccgtgc gcatcacctg
     1021 ccgctgatag cggatgaaga gttcttcaaa cgcctgggta tcgccggatt tgactcgcgc
     1081 caccagtgat tgcgccacgg gctgggcgtc tttccgcgcc tcatctcgcg acagcagggc
     1141 aatcatgg
//
LOCUS       DAOQDO010002395         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165417_length_1148_cov_2.625801, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002395 DAOQDO010000000
VERSION     DAOQDO010002395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165417_length_1148_cov_2.625801"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..434)
                     /locus_tag="PKZ53_27505"
                     /pseudo
     CDS             complement(213..434)
                     /locus_tag="PKZ53_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            627..>1148
                     /locus_tag="PKZ53_27510"
     CDS             627..>1148
                     /locus_tag="PKZ53_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNJ44262.1"
                     /translation="MSETTTARSTGYRGLTSAEVEASRQKHGINVLTPPERDPWWKLF
                     LEKFDDPVIRILIIAAVIAIGVGIAEGEYLEGIGIVVAILLATVLAFLNEYQANKEFD
                     ILNQVSDDVPVKVIRDGAYTTVPRKEIVVGDLVLLEAGEETPADGRLIEAVSLQIDES
                     RLTGESVPASKVAT"
BASE COUNT          338 a          266 c          248 g          296 t
ORIGIN      
        1 actaaaataa ggataagccc aatacttact acttaaaatg accaatgaaa aaacactggt
       61 caataaaaac agtccaattg caaaaggcat tttcagaaac ctatgattta tataagcaaa
      121 accgcccgat aagctaataa tgattgtcaa taagatatat gtactcatta ctttagaatt
      181 tattacgttt atatacttct acttgagcca aattcaatcg ttttttcaac cagttcttca
      241 cgagcatacc caaacagttt ttccgtctgt gaattgacca ggacgatttg gccctgatcg
      301 ttgacaatca gaatggcatc cggtgctaac tcaaacaact ccataaaggt tgtatcagaa
      361 gcagcaaatg gtttgatttt cttggactta cgtttcactc cagcccgctt tgaaggcgtg
      421 ccaacggatt ccataaatga aaattctcct taaaagatga aggacggagg taaaaacagg
      481 tcaggcattc tcggaaattt gagcatgaaa gaaacagagt ttgttcgttt tgcggcccca
      541 attgcaaccc tgggtcgcaa tctcccccca tttcaataca atatccaatt tgaacgaatc
      601 atcttgagct ccacattagg aaaactatga gtgaaacaac aaccgcacga agcacaggat
      661 accgaggact gacttctgcc gaggttgaag ccagccggca gaaacacggc attaacgtac
      721 tgactccacc tgagcgtgat ccgtggtgga aacttttttt ggaaaaattt gatgatccag
      781 ttatccgaat tctcatcatt gcggcagtca tcgccattgg ggtgggcatt gccgaaggtg
      841 aatatctgga aggcattggc attgtggttg ccattttgct cgccaccgtg ctggcctttc
      901 tcaatgaata ccaggccaac aaagagtttg acatcctcaa tcaggtcagc gatgacgttc
      961 cggtcaaggt gatccgcgat ggcgcgtaca caaccgttcc ccgcaaggaa attgtggttg
     1021 gggatctggt cctgctcgaa gccggtgaag aaacccctgc tgacggacgc ttgattgaag
     1081 ccgtttcgct ccaaattgac gaatcccggc tgactgggga atcggttccg gcttcaaaag
     1141 tcgccact
//
LOCUS       DAOQDO010002396         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165504_length_1148_cov_2.016468, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002396 DAOQDO010000000
VERSION     DAOQDO010002396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165504_length_1148_cov_2.016468"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PKZ53_27515"
     CDS             complement(<1..1126)
                     /locus_tag="PKZ53_27515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ44263.1"
                     /translation="MTLVKAETPNPEVSGTTGERKILYWQDPMNPANRSDKPGKAPDG
                     MDLVPVYAEEGSQPQMPPGTVKISAAKQQLIGVTYGEVTSDSLTHTIRTVGRVAYDET
                     KITRIHTKIDGWLDKVFVDFTGKLVTKGQPLISLYSPDLVSTQQEYLLALKAKNYLGQ
                     SEYKEIATSTNSLFESSRKRLLLWDIPEEEIQQIEKRGEPMRTLTLYAPSDGFVLAKN
                     AYERQRVTPETELYTIADLSNIWVLADIYEYEIPSVKLGQSTTVTFSAFPGETFRGKI
                     TYIYPQLDNMTRTLKVRIELPNSGFKIKPDMYANIELQIGYGEQLSIPEEAVLDSGSE
                     QTVFVVLEGGYFEPRNVQLGAKVGNRFIILNGLNVGEKIVT"
BASE COUNT          274 a          274 c          270 g          330 t
ORIGIN      
        1 aagtgacaat tttttcgccg acgtttaaac cattgagaat aatgaatcgg ttaccgacct
       61 tggccccaag ttgtacgtta cgtggttcaa aatacccacc ttccagcaca acaaaaacgg
      121 tttgctcgct cccggaatcc aatactgcct cctcaggaat ggagagctgt tcgccgtaac
      181 caatctgaag ttcaatgttg gcatacatat ctggcttgat tttgaacccg gaatttggaa
      241 gctcgatccg tactttcaat gttcgggtca tgttgtcaag ttgcggatag atgtaggtga
      301 ttttgccgcg aaatgtttcc cccggaaatg ctgaaaaagt cacggtggtc gactggccaa
      361 gcttgacgga cgggatttcg tattcataaa tgtcagccaa cacccagatg ttggagagat
      421 ctgcaatcgt atacaattcc gtctcaggag tcacccgttg ccgttcatac gcatttttag
      481 ccaaaacgaa cccgtcactg ggggcataaa gagtgagtgt tcgcattggc tcaccccgtt
      541 tttcaatttg ttggatctcc tcctcaggta tatcccacaa caacaatcgc tttcggctgg
      601 attcaaacaa tgagttcgtg ctggtcgcaa tttccttata ttcgctctgc ccgaggtaat
      661 tttttgcttt gagtgccagc aggtattcct gctgtgtaga aaccagatcc gggctgtaga
      721 gactaatgag cggttggcct ttcgtgacga gtttcccagt aaaatcaaca aaaactttgt
      781 caagccagcc atcaattttg gtgtggatac gtgtgatttt ggtttcgtca taggccacac
      841 gccctacagt ccgaatggta tgggtgagag aatctgaagt tacctcacca taagtgacgc
      901 caatgagttg ctgtttcgcc gcactaattt tcacggttcc gggaggcatt tgtggttgag
      961 agccttcttc agcataaact gggaccaaat ccattccatc cggcgctttc ccaggcttgt
     1021 ctgaacggtt tgccgggttc attgggtctt gccagtaaag aatttttcgt tcacctgttg
     1081 ttcctgaaac ctccgggttg ggagtttcgg ccttaaccaa tgtcatattg caaatcggac
     1141 aggtccct
//
LOCUS       DAOQDO010002397         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165702_length_1147_cov_2.760073, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002397 DAOQDO010000000
VERSION     DAOQDO010002397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165702_length_1147_cov_2.760073"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..233
                     /locus_tag="PKZ53_27520"
     CDS             21..233
                     /locus_tag="PKZ53_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ44264.1"
                     /translation="MNKYEVIIYWSNEDGIYVAEVPELPGCLAHGKSQAEALVNVQEA
                     VQLWIDTANEFGDPIPEPKGERLIFA"
     gene            complement(252..>1147)
                     /locus_tag="PKZ53_27525"
     CDS             complement(252..>1147)
                     /locus_tag="PKZ53_27525"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ44265.1"
                     /translation="RTRQPKVAPNAPRGGGGPGGFKSAHSTGFNSAPKNSFHESKNES
                     LSPTGRNTRADLPKKSDKPSNQQIRAEWDKHDGIIQKVVAEMNQKFQVKHYDFSPFIP
                     LDWKIIKAMVWVESSGPSWQSREGGVKPAPAFYGPRPIQFGNSDDPGLGVIRNGEELS
                     HLISSKELRDQLTATMNPDLCIRGGVALVFHYAAIYENAKIIDRQAVQHDTVRTKDTL
                     SKIAARNQTLVEEITRASNLSEKTILRVGQTVWFRSAHREWQLNGWKDWWIAVTKYNG
                     GGDPHYRSKVRAAYQAIVSES"
BASE COUNT          263 a          272 c          285 g          327 t
ORIGIN      
        1 aaactcggac gcgaggaaat atgaataagt atgaagtgat catctattgg agcaacgaag
       61 atggcatcta tgtcgctgag gttcctgagt tgcctggttg cctggctcat ggaaagagtc
      121 aggccgaagc gctggtaaat gttcaagaag cagttcaact ctggattgac actgccaatg
      181 aatttggcga tcccattcct gagccgaaag gtgagcggtt gatttttgcc tgaccaagtt
      241 cggttgaaag cttacgactc actgacaatt gcctgataag cggctcggac tttcgaccga
      301 tagtgtggat cacccccacc attatatttt gtgacagcaa tccaccagtc tttccatcca
      361 ttgagttgcc attctctgtg cgcagaacga aaccataccg tttgacccac ccgcagaatt
      421 gttttttcag atagattgct ggcacgggta atttcctcca ccagtgtctg gtttcgggcc
      481 gcgatttttg acaaggtatc cttcgttctg actgtatcgt gttgaactgc ctgacgatca
      541 atgattttgg cattttcata aatcgctgcg tagtgaaaca ccagtgccac gccgcctctg
      601 atacataaat caggattcat cgttgcggtg agttgatcgc gcaattcttt tgagctgatc
      661 aaatgagaga gctcctctcc attccgaatg acccctaagc cagggtcgtc tgaattccca
      721 aattgtattg gccgtggccc ataaaacgcc ggcgccggtt tcactccacc ctctcgtgat
      781 tgccagcttg gaccactact ctcaacccag accatggctt tgatgatttt ccaatccagt
      841 ggaataaatg gtgagaaatc ataatgcttc acctgaaact tctggttcat ttcggcaacg
      901 accttttgaa tgatgccgtc gtgtttgtcc cattcggctc ggatttgctg gttggacggt
      961 ttatcagatt ttttgggaag gtccgcccgt gtgtttcttc cggttggtga caaagattca
     1021 tttttggatt cgtgaaatga attcttggga gcggaattaa atccggttga atgggctgat
     1081 ttaaatccgc cggggccgcc gccaccgcgt ggggcattgg gagcaacttt tggctgtcgg
     1141 gtacgag
//
LOCUS       DAOQDO010002398         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_165976_length_1146_cov_3.662695, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002398 DAOQDO010000000
VERSION     DAOQDO010002398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165976_length_1146_cov_3.662695"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..320)
                     /locus_tag="PKZ53_27530"
     tRNA            complement(247..320)
                     /locus_tag="PKZ53_27530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(284..286),aa:Arg,seq:acg)
     gene            complement(331..414)
                     /locus_tag="PKZ53_27535"
     tRNA            complement(331..414)
                     /locus_tag="PKZ53_27535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(378..380),aa:Leu,seq:cag)
     gene            complement(591..>1146)
                     /locus_tag="PKZ53_27540"
     CDS             complement(591..>1146)
                     /locus_tag="PKZ53_27540"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNJ44266.1"
                     /translation="GTNFAFLGDEFYIRAKQPIPSTRHYGPYPQIEDGVGMVRRFLNS
                     FRTTFKQRLKRPGIADELRQVTTLATGTLFYPTLAAAAEEINQAFGTQLHVVPVTNEY
                     FGPEVNVSGLLTATDFLAARDQFKGQKLFIPGESVMGENQIFLDGMTLADLRTRLGMD
                     IQLGGITGKDFVLTVLGAGRKTAG"
BASE COUNT          261 a          332 c          289 g          264 t
ORIGIN      
        1 ggaaataaag gatttgtcag taccacccgc gtgagccggt ggggtttttg gaaacactca
       61 ggcccaccca ctgacgcggg tggtactgac tgggtcgctt atcttatttt tctccacaca
      121 aaatccagta gaagcagagg tcatcttgct gccacaggcg tcgtgaaact cccgactggc
      181 ttttctgaac cccaaaaagt aaaaagcccg ctctggcaag cgggctcaat ctcttgattc
      241 taaaagtaca cccgggaaga ctcgaacttc cgacctcttg attcgtagtc aagcgctcta
      301 tccaactgag ctacgggtgc aaatggggaa tgcggatgag aggacttgaa cctccaaggg
      361 ccgaagccca catggtcctg agccatgcgc gtctgccaat tccgccacat ccgcaaagga
      421 acgcgcatta tcgtcacgaa atgcccgttt gtcaactacc ttgttcaaat ttttttcggg
      481 cttggggctg aagaaggcgg gctcaggata ttgaacccgt tttcttccgc cccgagcttg
      541 cgagtcttca accccaagcc ctcagcccca caccctcagc cctggttttt tcagcccgct
      601 gtcttccgcc ctgctcccag caccgtgagc acaaagtcct tccccgtaat gccgccgagc
      661 tggatatcca tcccaagtct ggttcgcaaa tccgccaggg tcataccgtc gaggaaaatc
      721 tgattctcgc ccatcactga ttcgccgggg atgaacaact tctggccttt gaattgatcg
      781 cgggctgcca gaaaatcagt cgcagtcagc aaccccgaca cgttcacttc cggaccgaaa
      841 tattcatttg tgaccgggac cacgtggagt tgcgttccaa aggcttgatt gatttcctcg
      901 gcagcagcag ccagcgttgg gtaaaagagt gtcccggtgg ccagtgtcgt cacctggcgt
      961 aactcgtcgg cgatgccagg tcgtttcaga cgctgtttga aggttgttcg aaaactgttg
     1021 agaaaccgcc gcaccatacc cacgccgtct tcaatctgtg gatacggtcc atagtggcgt
     1081 gttgaaggaa tcggctgttt ggcccggatg taaaactcgt cgcccaaaaa ggcaaagttc
     1141 gtgcca
//
LOCUS       DAOQDO010002399         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_166142_length_1146_cov_1.923006, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002399 DAOQDO010000000
VERSION     DAOQDO010002399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166142_length_1146_cov_1.923006"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKZ53_27545"
     CDS             complement(<1..458)
                     /locus_tag="PKZ53_27545"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ44267.1"
                     /translation="MYCPACGTDLPSQLKFCNRCGTQLSAQGKPVEIAIPSNQLTEMV
                     KYVSTTVGVIGGAALILLFVFALKLLERGQVGEAVPLFLIGLSMIITLAGVFLFQWSR
                     LLKLYLHIVPPVQSNSLPLPERPLVQPNLLPEPPISVTEHTTKTLQPQSQ"
     gene            complement(614..>1146)
                     /locus_tag="PKZ53_27550"
     CDS             complement(614..>1146)
                     /locus_tag="PKZ53_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44268.1"
                     /translation="GLSPKDFKLALDFETSRDTALHLPFHDYQVIHFATHGLLNSQNP
                     GLSGIVLSLVNNQGADVDGFLRVADLINLKIPVELVVLSACRTGLGKEVRGEGLVGLT
                     RAFMYAGASRVVASLWKVDDKATAVFMKEFYRAMFQEHQRPAAALRTAQLALMKNPRF
                     RSPFYWAAFTLQGEWR"
BASE COUNT          290 a          287 c          299 g          270 t
ORIGIN      
        1 tactgcgatt gtggttgcaa ggtcttggtg gtgtgctcgg tgacgctgat cggtggctct
       61 ggaagcaggt tcggttgaac cagaggtcgc tctggcaagg ggagggaatt cgattgtacc
      121 ggaggaacaa tgtgcaaata gagtttcaac agtcgagacc attgaaagag aaacacaccc
      181 gcgagagtta taatcattga caggccaatc agaaataagg ggactgcttc gccaacctgc
      241 ccccgttcca gtaacttgag ggcaaaaaca aacagcagaa tcagtgccgc gcctccgata
      301 acaccaaccg tggttgacac atatttcacc atctctgtca gttgatttga tggtattgct
      361 atctcgacag gttttccctg ggctgacaat tgggttccac accggttgca gaattttaat
      421 tgtgagggta agtcggttcc acaggctggg cagtacataa ctcaaagttc tccttcaaaa
      481 aaacaaacca taccttaccc gcttgtttgc tgggaagtgt tttctttgtg agatgttacc
      541 gaaataccgg gttgcgacca gtcggtacgc gttgccgatg gatccggttt gaatcaatca
      601 caatgaccac ggttcatcgc cattcgccct ggagcgtaaa cgctgcccaa taaaatggag
      661 accgaaaccg ggggtttttc atcagtgcca gttgtgctgt tcgcagggct gcggctggac
      721 gttgatgctc ctggaacatc gctcgataaa attccttcat aaagacggcg gtggccttat
      781 cgtcaacctt ccacaggctg gcgaccacgc ggctggcgcc ggcatacata aaggcacgcg
      841 tcagcccgac cagcccttca ccacggactt cttttccgag accagtacgg caggcactca
      901 ggaccaccag ttccaccggg attttcaggt tgatcagatc tgcaacccgc aaaaacccat
      961 ctacgtctgc accttgatta ttgaccagcg acaggacaat tcctgaaagc cccggattct
     1021 gactgttgag gagtccgtgg gtggcaaaat gaatcacctg ataatcatga aatggaagat
     1081 ggagggccgt atctcggctg gtttcaaaat ccagagcgag tttaaagtcc tttggtgaaa
     1141 gaccgc
//
LOCUS       DAOQDO010002400         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_166589_length_1144_cov_3.067952, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002400 DAOQDO010000000
VERSION     DAOQDO010002400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166589_length_1144_cov_3.067952"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKZ53_27555"
     CDS             complement(<1..682)
                     /locus_tag="PKZ53_27555"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNJ44269.1"
                     /translation="MKILFVTLCILFGVLPVIAQVSGNQTYSPQNQGPKRNVQPMVSD
                     GRPTVDRDHLFVDANILMNVKAEEFVAMFGVAQEAATPAECSQKLDAKIADFSSRMKS
                     LGINQADIFVDYTTQNRIYDFNLSKGVAKEKAAGFEAKRTVSVHYTKSDLFDQIVANA
                     SAAGVFDLVKVDYIVKDVSKIKNALLEAAQKVIKDKEARYGTLFGIKVGSRMQVDMEK
                     YSVYYPAEL"
     gene            complement(799..>1144)
                     /locus_tag="PKZ53_27560"
     CDS             complement(799..>1144)
                     /locus_tag="PKZ53_27560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44270.1"
                     /translation="VRAGGGEIEGNGVGDSLFPLAAKSKQTTFYQYKLKDIQLVKDAT
                     EHSVIKIDRFNFGIRLPINVSSGDGSSQVQYSDVGFNTALSVREGEKIVVGTTNSSDG
                     AIVVVVSVKQIQ"
BASE COUNT          269 a          294 c          251 g          330 t
ORIGIN      
        1 acagttctgc cgggtaatag acgctgtatt tttccatatc cacctgcatt cgcgagccaa
       61 ctttaatgcc aaacaatgtg ccataccggg cttctttgtc cttgatgacc ttttgagcgg
      121 cttcaagcag ggcatttttg attttgctga cgtctttgac gatgtaatcc acctttacca
      181 gatcaaaaac tcctgcggca ctcgcgttag ccacaatctg gtcaaacagg tcgcttttgg
      241 tgtagtgaac tgaaacggtg cgtttggctt cgaatccggc ggctttttct ttggcgaccc
      301 ctttggaaag gttgaaatca tagattcggt tttgcgtggt gtaatccaca aaaatatcag
      361 cctgattgat tcctaaactc ttcatccgac tggagaaatc ggcaattttc gcgtccagtt
      421 tctgactgca ttcggctggg gttgcggctt cttgagcgac gccaaacatc gcgacaaatt
      481 cttcggcttt gacattcatc aggatattgg catcaacgaa gagatgatcc cggtctacgg
      541 ttgggcgtcc gtccgacacc atcggctgaa cattgcgttt cggcccttga ttttgaggtg
      601 aataggtttg attgcccgaa acctgggcga taacaggcag aactccgaat aaaatacaca
      661 gtgtgacaaa tagaattttc ataatagcct cttggttctt ggttcttggt gcttcgtgct
      721 tcgtgctttg tacctggtct tcaaaggtat tgtctccaaa acgccaggta ccaagcacca
      781 gtcaccacgt acttcccctt attgaatttg cttgacgctc accacaacca caatcgcacc
      841 gtcgcttgaa ttggtcgttc ccaccacaat tttttcgcct tcgcgcaccg acaaggccgt
      901 gttaaatccg acatcgctat attgaacctg agaagaccca tcccctgagg acacgttgat
      961 tgggagccgg ataccaaaat taaaacgatc aatcttgatg accgagtgtt ctgtggcatc
     1021 tttcaccaat tggatatcct tgagtttata ctgatagaaa gttgtctgtt tgctttttgc
     1081 cgcgagcggg aaaaggctat ccccaacacc attcccctca atttctcctc cgccagctcg
     1141 aacc
//
LOCUS       DAOQDO010002401         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_166926_length_1143_cov_2.217831, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002401 DAOQDO010000000
VERSION     DAOQDO010002401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166926_length_1143_cov_2.217831"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKZ53_27565"
     CDS             <1..278
                     /locus_tag="PKZ53_27565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44271.1"
                     /translation="GLSLINRSKLGYIAILAFSLLPALAILNNVALLLASIAKGTVFS
                     TPGFTMLGLVSCVEVWVVGYLIFNLISLDVRNHVWGEPKASNDATLD"
     gene            459..1052
                     /locus_tag="PKZ53_27570"
     CDS             459..1052
                     /locus_tag="PKZ53_27570"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNJ44272.1"
                     /translation="MSSPKCNILIPNQILTIDDFCTSAECQDFIQRAERVGFDEAPIT
                     TATGFVRRPSVRNNTRVMIDDPDLAESLWERVRSFIPAELEGCVPVGLNERFRFYRYD
                     ENQFFAPHYDGCFRRPNGEESLLTLMVYLNEDFVGGETRFDLKFPHPDISILPKEGMV
                     LCFFHYLRHEGAPVGMGRKYVLRTDVMFRKIKTDTSQ"
BASE COUNT          274 a          253 c          280 g          336 t
ORIGIN      
        1 atgggctttc gctcatcaac cgaagcaagc tcggatacat cgccattctg gctttttcgt
       61 tgttgccagc cctggctatc ttaaataatg tcgccctgct gctggcttcg atagcgaaag
      121 gaactgtatt ttcaacccct ggatttacca tgctggggtt ggtcagttgt gttgaagtct
      181 gggtggttgg atatttgata ttcaacctga tttctttgga cgtcagaaac cacgtttggg
      241 gcgaaccaaa ggcgagtaat gatgccactt tggactgagg actgaggaaa atacaatctt
      301 ttcagtgatt tagcttttct gtaacatgag aattaatagt ataaatctca cgcctgaagg
      361 cgttactctg aactctgaaa ccctgcagct tgtagtttgt ttcagccctg agccccaagc
      421 cctaagccct ggattttcaa cccggaaccc taaagattat gtcttctcca aaatgtaaca
      481 ttctcattcc aaatcagata ctgacgattg atgatttttg tacttcggct gaatgccagg
      541 attttattca gcgggcggaa cgtgtcggct ttgatgaggc gccgattacg accgcgaccg
      601 gatttgtccg gcgtccgtca gttcgaaaca atacgcgggt gatgattgac gatcctgatc
      661 tggctgaaag cctgtgggaa cgggtcaggt catttattcc ggctgagctt gaaggctgtg
      721 tcccggttgg tctcaacgaa cggtttcgat tttaccggta tgacgaaaat caatttttcg
      781 ccccgcatta tgatggctgt ttccgtcggc caaacggaga agaaagcctg ctcacattga
      841 tggtgtatct caatgaggat tttgttggcg gagaaacccg ctttgatctc aaattcccgc
      901 acccggacat ttctattttg cccaaagagg gaatggtgct gtgctttttc cattacctgc
      961 ggcacgaagg tgcaccggtt gggatgggcc gaaagtatgt acttcgcacc gatgtcatgt
     1021 ttcgcaaaat aaaaactgat accagtcagt agccaatagt cagtagttcg ttaagcctat
     1081 ttgattgaat tacttgactg tctattaaca caatgatcag caatgctttt gtttgtggag
     1141 cgg
//
LOCUS       DAOQDO010002402         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_167138_length_1142_cov_2.963201, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002402 DAOQDO010000000
VERSION     DAOQDO010002402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167138_length_1142_cov_2.963201"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1018
                     /locus_tag="PKZ53_27575"
     CDS             200..1018
                     /locus_tag="PKZ53_27575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ44273.1"
                     /translation="MPLFSSDELTLPPVFTNCIGMEFVLIPTGSFLMGSTDAEMEAVL
                     ANAKRYDSDAKREWFTPEQRQHRVTLSQPFYLGKFQVTQAEWQVVMGNNPSYFEGERL
                     PVEQVSWDDSQKFLKKLNETEDGYLYRLPSEAEWEYACRAGTTTPFSFGETITTHQVN
                     YDGEYPYGNAPTGECRHTTTPVGTFPANKWGLHDMHGNVCEWCQDTWQEDYNGSPVDG
                     SAWEKDSYRGRVMRGGSWLYGPWFCRSASRLWDCAESHGWYYGFRVVAMRTLST"
BASE COUNT          270 a          267 c          305 g          300 t
ORIGIN      
        1 gccaactggc ccaactccac caaacgacga ttgaagaggt gggccgcatc acaacggcga
       61 acttcaaacg gttgtttggg ataggttcag agtccagcct ttaggcggtg gtattgatac
      121 gattcggggt tcagggaaat acctttttct tcaataagct cgttttcctg caatacaatc
      181 tgactgttcc aaagcggtta tgcctttgtt ctcatctgat gaattaacgc ttcctccagt
      241 ttttaccaat tgcattggga tggaatttgt actgattcca accggcagtt ttctaatggg
      301 ctcgacagat gctgagatgg aggctgtttt ggctaatgcg aagcggtatg actcggacgc
      361 caaacgcgaa tggtttaccc ctgagcaacg ccagcatcga gtaactttga gtcagccttt
      421 ttacctggga aaattccagg tgacccaggc tgagtggcag gtcgtgatgg ggaacaatcc
      481 ttcctatttt gaaggagaga gattacccgt ggaacaggtg tcatgggatg acagccagaa
      541 atttctgaaa aaactgaacg aaacggagga tggctacctg taccggttgc ccagtgaagc
      601 cgaatgggaa tatgcctgcc gcgctggaac gaccacccct ttttcgtttg gagaaaccat
      661 caccactcat caggtaaatt atgatgggga atacccttat gggaatgctc ccaccgggga
      721 atgtcgccac accacaaccc cggttggaac ttttcctgcc aataagtggg ggcttcacga
      781 tatgcacgga aatgtgtgcg aatggtgtca ggacacgtgg caggaggatt acaacgggtc
      841 cccggttgat ggaagtgcct gggaaaaaga cagttatcgt ggccgcgtca tgcgtggagg
      901 ctcgtggctc tacggcccat ggttttgccg atcagcgagt cgtctctggg actgcgctga
      961 atcccatggc tggtactacg gttttcgggt ggttgcgatg aggactctca gtacctgatg
     1021 cactttgatt tatgcactct tgaccgcgcg ttttttgcgc ggttccgaaa tttttagctc
     1081 ccactcgtca atgattttag agttcaatct ttcgcttttc aaaaacctgc tgatacgatt
     1141 ca
//
LOCUS       DAOQDO010002403         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_167412_length_1141_cov_7.067219, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002403 DAOQDO010000000
VERSION     DAOQDO010002403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167412_length_1141_cov_7.067219"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKZ53_27580"
     CDS             <1..291
                     /locus_tag="PKZ53_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44274.1"
                     /translation="FCLGLGMFPMLVSLGFGLATWFHVPVLSGLNGLLVTLFFALVGL
                     QCWVLGLAANLLAVNRRLLEELLVRERRAAVQPLGTQVRDTSPQTVCDPEVN"
     gene            270..659
                     /locus_tag="PKZ53_27585"
     CDS             270..659
                     /locus_tag="PKZ53_27585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44275.1"
                     /translation="MRSRSELNRELWGKKKRKMKPGMSFPAVVQALATGKYQLPHSPE
                     VRPFIGKSSQAFEIFGKGKRFIPPIDLSALSGCEKSVAGERNGALSPGGSQGGARPLV
                     GIHGKANFPHPTLFPEIHFVINCQYLL"
     gene            681..>1141
                     /locus_tag="PKZ53_27590"
     CDS             681..>1141
                     /locus_tag="PKZ53_27590"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNJ44276.1"
                     /translation="MTHMEELTELLHQRGMIRPRDAAKLGIAPEYFQRLSAAGRIERV
                     SRGLYRLVEAPLSEHHDLALVAARVPHGTICLLSALAFHRLGTQLPFEVWLAVGRNAG
                     VPRLEFPHIRLMRFSADAFTTGVETHLIEGVQVKVFSPAKTVADCFKFRNQ"
BASE COUNT          239 a          283 c          322 g          297 t
ORIGIN      
        1 ttttgcctgg ggttggggat gtttccgatg ctggtatccc ttgggtttgg attggcaacc
       61 tggtttcacg tgccagttct ttctggactg aatggactgc tggtgacttt gttctttgca
      121 ctggtggggc ttcagtgctg ggtgttgggc ctggccgcca acctgctcgc ggtcaaccgg
      181 cgcttattgg aagaactcct ggttcgggaa cggcgggctg ccgttcaacc ccttgggact
      241 caggtgcgcg acacctcgcc tcagaccgtt tgcgatcccg aagtgaattg aatcgggagt
      301 tgtggggcaa gaaaaaaaga aaaatgaagc ctggcatgag tttccccgca gttgtgcaag
      361 ctctggcgac tggcaaatac cagttaccac actcgccaga agtcaggccg tttatcggta
      421 aaagcagtca agcatttgag atattcggaa agggaaagcg gttcattccc ccgatagatc
      481 tcagtgcctt atccggttgt gaaaaatcgg ttgctggtga acgcaatggt gctttgtcgc
      541 ctggaggcag tcaaggtggc gcccgccccc tggttggaat ccatggaaaa gctaatttcc
      601 cacatccaac attgtttcct gagatacatt ttgttataaa ttgccagtat ttattatgat
      661 ctggtggtat ttcataacat atgacccaca tggaagaact caccgaactg cttcatcaga
      721 gaggaatgat tcgtcctcgg gatgcggcaa agcttggaat cgcccctgaa tactttcagc
      781 ggctctccgc cgccggacgg atcgaacggg tttcgcgtgg gctgtatcgt ttggtggaag
      841 ccccactcag tgaacaccac gatctggccc tggttgccgc ccgagtcccg cacggaacga
      901 tttgtttatt gtcggcgctg gcctttcatc gtcttggaac tcaactgcca tttgaggtct
      961 ggctggcagt gggaagaaat gcgggcgttc cccggcttga gttcccacac atcaggttga
     1021 tgagattttc agccgacgcc tttacgactg gggtggaaac gcatctgatt gaaggcgttc
     1081 aggtcaaggt cttttctccg gccaaaaccg tggccgattg tttcaagttc cgcaaccaga
     1141 t
//
LOCUS       DAOQDO010002404         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_167971_length_1139_cov_4.281365, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002404 DAOQDO010000000
VERSION     DAOQDO010002404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167971_length_1139_cov_4.281365"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..>1139)
                     /locus_tag="PKZ53_27595"
     CDS             complement(155..>1139)
                     /locus_tag="PKZ53_27595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNJ44277.1"
                     /translation="IAGTLEFESLDPHAFDSTDRRVAEDIAMQISVAVRNTRLYHDAQ
                     AAREYLQTVLDVAVDTAIISTDTQGHIITFSRGAEILFGRNTEEMVGNQVDPLFDESS
                     AKATIEELFRTQSDEPWESDVRLVRQNGETFFAYVTVRPLRARQSPGYLFLLLDITQR
                     LNLQERLHRLSITDELTGLYNKRYFTTLLPREISRSERQRSPLVLCYFDLDGFKKFND
                     SKGHVQGDILLKWIGGLVQQAIRRNVDLPFRYGGDEFVLILPGTRAKDAAQVGERIRA
                     AVERQYHGEVTVSFGVAEFCGETADELTTKADHIMYQAKHAGGNQVCVAED"
BASE COUNT          305 a          297 c          285 g          252 t
ORIGIN      
        1 gaatgaagaa tgaagatagc ggttagtggt tagtggttag tggttagaaa tcaatatttt
       61 cgaagaagaa ccaagcacca agaaccaagc accaagcacc aagcaccaag caccaggaac
      121 caagaacttt cttctttctt cattcccaaa aggatcagtc ttcagccacg cacacctgat
      181 tcccaccagc gtgttttgcc tgatacataa tatgatcggc tttggtggtc agttcatcgg
      241 cagtttcacc acaaaattca gcaaccccaa aactgacggt tacttcgccg tggtactggc
      301 gctcaacggc ggcgcggatg cgctctccga cctgagcggc atctttggca cgggtaccag
      361 gcaagatcaa gacaaattca tcaccaccat accggaaagg aaggtcaaca ttgcgccgaa
      421 tggcctgctg aaccagccca ccaatccatt tcagcaaaat atccccctgg acgtggcctt
      481 tactgtcatt aaacttttta aatccatcaa gatcgaaata gcacaaaacc agcgggcttc
      541 gctggcgttc gctgcgggag atctcgcggg gcaggagcgt cgtgaagtat cgcttattat
      601 agaggccagt gagttcgtca gtgatgctga gacgatgcag acgttcctgc agattgaggc
      661 gctgggtgat atccagtaac agaaacagat acccagggct ttggcgtgcc cggagtgggc
      721 gaaccgtcac ataggcaaaa aaagtctcgc cgttttgccg gacaaggcga acatctgatt
      781 cccagggttc atccgattgg gttcggaaca gctcctcaat cgtcgccttg gcacttgatt
      841 catcaaagag cgggtcaacc tgatttccca ccatttcttc ggtattccga ccaaacagaa
      901 tttcggcgcc acgactgaag gtaataatgt gaccctgggt gtccgttgaa ataatagccg
      961 tatcaaccgc aacatcgagc accgtttgca gatattcgcg ggcagcctga gcgtcgtgat
     1021 agagccgggt attgcggacg gcaactgaaa tctgcatcgc aatgtcttcc gcaacccgac
     1081 ggtcggttga atcaaacgca tgaggatcaa ggctttcaaa ttcgagggtc ccggcaatg
//
LOCUS       DAOQDO010002405         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_168053_length_1139_cov_2.479705, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002405 DAOQDO010000000
VERSION     DAOQDO010002405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168053_length_1139_cov_2.479705"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>1139
                     /locus_tag="PKZ53_27600"
     CDS             196..>1139
                     /locus_tag="PKZ53_27600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44278.1"
                     /translation="MLAKQFHILGFSLLLVLLAGSLVSVWAAQALPVAGIESPVDVSQ
                     AWKFQTGDDLNWARPEFDDTAWPLVSLTQAWGKERYTSGFQWYRLTLRLPWAQSEAHR
                     HSAIAVALGKFEYGCYQVYAGGTLVGGYGHFSPSPHTLPGRTQVIEIPAAAIGPDGIC
                     VVAIRGWRDPGFERVNALMRERPVGNFLFGALPELRREAMLQEQQVWLANVPQLICLV
                     VFTLVGLYHLQLFRRRRELKEYFWFGLIPILASISIFFNSELYLGVVTPLRAWGLALG
                     PAFFNTVFWVQFYCLIFGWSFTRVIRLCQILPGCMAVIV"
BASE COUNT          233 a          268 c          325 g          313 t
ORIGIN      
        1 ggcaaaacac aaaatccggt agaagcaggg atattgaaag aattgtattt tcccgcaacc
       61 ctgaaccctg aaccctgaac cctgaacctt gcgcaaatac agtgattgaa gattgttgga
      121 aagtatggaa aatgaaggtc tcccgataac cccagccgcc atccgacaga gaacaaggag
      181 tccctcacga caccaatgct cgctaaacaa ttccacattt tgggattttc gctgctgctg
      241 gtgctgctgg ctgggagttt ggtgtcggtg tgggcggcgc aggcgcttcc agtggcaggg
      301 attgaatctc ccgttgatgt atcacaggcg tggaaatttc aaactggtga tgatctgaac
      361 tgggcccgac ctgagttcga cgatactgcc tggcctttgg tttcgttgac gcaagcctgg
      421 ggtaaggagc gctacacgag cggtttccag tggtatcggc tgacactccg attgccctgg
      481 gctcaaagcg aagcccaccg ccatagtgcg atagctgtgg cgctgggcaa atttgaatat
      541 ggctgttatc aggtttatgc cggaggaacg ctggttggcg ggtacggtca tttttcccca
      601 tctccgcata ccttgcctgg acggacacag gtaattgaaa ttcctgctgc cgcgattgga
      661 ccggatggaa tctgtgtggt ggcgatccgc gggtggcgcg atcctggttt tgaacgggtg
      721 aatgccctga tgcgagagcg gccagtcggg aactttctat ttggtgcttt gcccgaactc
      781 aggcgcgaag cgatgcttca ggagcaacaa gtctggctgg ccaatgttcc acaactgatt
      841 tgtctggttg tctttaccct ggttggactc tatcacctac agctttttcg ccgtcgccgg
      901 gaactgaagg aatatttctg gtttggactg atcccaattt tggcttcgat cagtattttt
      961 tttaattcag agttatatct gggcgtggtg acgccgcttc gagcctgggg actggccctg
     1021 ggacccgctt tttttaatac tgtcttttgg gttcagtttt attgtttgat ctttggatgg
     1081 tcctttacgc gagttatccg gttgtgtcag atattgcccg gctgtatggc ggtgatcgt
//
LOCUS       DAOQDO010002406         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_168379_length_1138_cov_2.298246, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002406 DAOQDO010000000
VERSION     DAOQDO010002406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168379_length_1138_cov_2.298246"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..>1138
                     /locus_tag="PKZ53_27605"
     CDS             412..>1138
                     /locus_tag="PKZ53_27605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44279.1"
                     /translation="MRLSFQQFTRTTCGVVCLNLMVLTTLPPVWAQPSSITPIRLKSI
                     LPQDQPQDIIVGKIIEYEVSNQKPQVHLFSLKMGEYLEFEIQSTNQHLKISRLKPGTG
                     WDESVRAPNGSQTVLESWVLAEATGLHQFKVETTDPTVKYTLKIVEYRPATEADWHLL
                     KATQVIAEGTELKVLQTKEAILQAIEKYKLAVKECQLAGNLSCEAELVNSIGNLYYNF
                     GELAQAREYYEKSLEISRTTNDPS"
BASE COUNT          338 a          270 c          244 g          286 t
ORIGIN      
        1 ggcggcgttc cagctcaccg gtgaatggaa ataactggtt caaggcagaa aaaatggaat
       61 aaaactataa aagggttcag atcattcttt ccgtgtctgg tcttaaaaaa tccaggtaaa
      121 tcgttgaaaa gtctaaagtt cccttctatg tgcaaaatgt attctgtggt cttttcttat
      181 gagatgttga tagcctaacc tcttgctagc cacagacctg tgaatttcag gacctcggtg
      241 gagccacctt tcttttcgag cgacaaccac agtacacttg gtgccagttg ctgcagatat
      301 cttcaccttg cgctcgcttg ctaaaaactc aaaatattca aacgcctggt ggttactcca
      361 aaagttgatc caaggtgatc aaaccaagct ccaaaattaa aacagaatag tatgcggctt
      421 tcttttcagc agtttaccag gactacctgt ggtgtggtat gtctcaatct catggttttg
      481 acaaccctgc ctccagtatg ggcacagccg tccagcatca cgccaattcg attgaaatct
      541 atccttcccc aggaccagcc tcaagacatc atcgttggaa aaataatcga atatgaagtt
      601 tccaatcaaa aacctcaggt tcatctattc agcctgaaaa tgggcgaata tcttgagttt
      661 gagattcaat caaccaacca gcatctcaaa atcagtcggt taaaacctgg cacagggtgg
      721 gatgaatccg ttcgggctcc caatggatca caaaccgttc ttgaatcctg ggttctggct
      781 gaagcaaccg ggctgcacca gttcaaggtt gaaacgaccg accctacggt caaatacacc
      841 ctcaagattg tggaatatcg cccggcgacg gaagccgact ggcaccttct caaagccacc
      901 caagtgattg ccgaaggaac cgagttgaag gtattacaaa cgaaagaggc aattttacaa
      961 gcaattgaaa aatacaagct agcggtcaaa gaatgccaac tggctggcaa tctgagttgc
     1021 gaagcagagc ttgtgaatag cattggcaat ttgtactaca attttggcga actggcccag
     1081 gcacgtgaat attatgagaa atcgcttgaa atttcccgca ccaccaatga tcccagtt
//
LOCUS       DAOQDO010002407         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_168651_length_1137_cov_2.347505, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002407 DAOQDO010000000
VERSION     DAOQDO010002407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168651_length_1137_cov_2.347505"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1137
                     /locus_tag="PKZ53_27610"
     CDS             28..>1137
                     /locus_tag="PKZ53_27610"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HNJ44280.1"
                     /translation="MKRGQEAQKLHVLNRPEANRQQTGLRIILRGTDQLEENQQAKQA
                     FFNAAARWEAVIQTPITMVLDVDYGPTRFGVPFGSGVIGSTFTSDTTVIYSELRKRLI
                     AGAIGTTDEAIYRALPEDALPTEIGPATRIELPVTVARAVGLAPIAASETGGGAPSIG
                     FNSNFNFDFSPENGIGSGQTDFDAVTVHEIGHALGFTSNVGLTELTGGTVPVPTIWDL
                     YRFGNEITLSEFTSAERLQLTGGTQILFSGNDLKGLSLSTGAPNASGGDRRQASHWKD
                     DSILGRRIGIMDPTLGSGDRSVITDNDLRALDLMGYRMAGQTGGDTVPPAVSIVSPNG
                     GETVFVGQQSTITWTSSDNGLIVSQDIALSTDGGAT"
BASE COUNT          270 a          311 c          294 g          262 t
ORIGIN      
        1 gaaacttccc cttctgaaac catgctgatg aaacgtggcc aggaagctca aaaactccac
       61 gttttgaacc gccccgaagc caaccggcaa caaaccggcc tgcggattat tttacgtggt
      121 acagatcaac tcgaagaaaa tcaacaggcc aaacaggcgt tttttaatgc cgccgcccgc
      181 tgggaagccg tcattcaaac accaattacc atggtgctgg acgttgacta tggtccaacc
      241 cggtttggtg tcccgtttgg ttcaggagtt attgggtcaa cgtttacctc cgacacaacg
      301 gtcatctata gcgaactgcg caaacggctg atcgctggtg ccattggaac aactgatgag
      361 gcaatctacc gcgccttgcc ggaagacgcc ctcccaaccg aaattggtcc ggcaacccgc
      421 atcgaactcc ctgtaaccgt cgctcgcgct gttgggttgg cgcccattgc cgccagcgaa
      481 acgggtggcg gggcaccgtc aattggattt aattccaact ttaattttga tttttcacct
      541 gaaaacggaa tcggatctgg tcagactgat tttgatgccg tcaccgtcca cgaaatcggt
      601 catgcgctcg ggtttacgtc caatgtaggg ttaaccgaat taactggtgg cacggttcca
      661 gttccaacca tttgggatct gtatcgcttt ggcaatgaaa tcaccttgag cgagtttacc
      721 tcagccgaac gacttcaatt gacgggtgga acacagatcc tgttttccgg gaacgacctc
      781 aaagggttat cgctctcaac cggcgctccc aatgccagcg gcggtgatcg ccgacaggcc
      841 agtcactgga aagacgacag catcctgggc cgacgcattg ggattatgga tccaaccctt
      901 ggcagcggag accgctcagt cattactgac aatgatttgc gcgccctcga tttgatggga
      961 tatcgcatgg ctggccagac gggcggtgac acagtgccac ccgcagtttc aattgtttcg
     1021 ccaaatggtg gtgaaaccgt gtttgtcggc cagcagtcaa ccattacctg gacctcaagc
     1081 gataatggat tgatcgtgtc acaggatatt gcgctttcaa ctgatggcgg agcgacg
//
LOCUS       DAOQDO010002408         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_168904_length_1136_cov_2.927845, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002408 DAOQDO010000000
VERSION     DAOQDO010002408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168904_length_1136_cov_2.927845"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1136
                     /locus_tag="PKZ53_27615"
     CDS             <1..>1136
                     /locus_tag="PKZ53_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HNJ44281.1"
                     /translation="IANRLGLVFMKINGPALGHKVTSLDPMEAPNATARQEIEKLNLA
                     LEMGNNVMLYIDDIQHTNPEFLQKFISLCDAQRKIEGVWNGQTQTYDLRGKRFCVCMA
                     GNPYTESGQKFQIPDMLANRSDTYNLGDILTGRETLFELSYLENALTSNRVLAPLTTR
                     EPGDVYKLVQMAKGEAVQSDQLSHGYSGVEIGEMVSVLKNLLQIQQTVLKVNQQYIRS
                     ASQDDAYRTEPPFKLQGSYRNMNKLAEKVVAVMNPEELENLLDDHYLGEAQILTTSAE
                     HNLLKLAELRGRMSPEQTSRWNEIKRGFARVKVMGGKDDDPVVKVTGQIGLVSERLED
                     ICAALTTLHSVTARHTEQSARSGDLSTYLTKLDQTLNVLRRVQS"
BASE COUNT          313 a          290 c          293 g          240 t
ORIGIN      
        1 acattgccaa ccgacttggg ttggtgttta tgaaaatcaa tggacccgcg ctcggccata
       61 aagtcacgtc gctggatccg atggaagctc caaatgccac ggcgcgccag gaaatcgaaa
      121 aactcaatct ggcgctcgaa atgggcaaca acgtgatgct ctatatagat gacatccagc
      181 acacaaaccc tgaatttctg caaaaattta tttccctgtg tgatgcccag cgcaaaatcg
      241 aaggcgtctg gaatggtcag acacaaacct atgacctgcg cggaaaacga ttttgcgtct
      301 gtatggctgg aaacccgtac accgaatcag gccagaaatt ccagattccg gatatgctgg
      361 cgaaccggtc tgatacctat aacttaggcg atattttgac aggccgtgaa acactgtttg
      421 aactcagtta tctggagaat gccctgacct caaatcgggt gctggctccg ctgaccaccc
      481 gtgaaccagg tgatgtgtac aaactggtcc aaatggccaa aggtgaagcg gttcaaagcg
      541 atcagctttc gcatggatat tccggtgttg aaatcggcga gatggtcagc gttttgaaaa
      601 acctgctcca gattcaacag accgtgctca aggtcaacca gcaatacatc cggtcagctt
      661 ctcaagatga cgcctatcga accgaaccgc cgttcaagct tcagggaagc tatcggaaca
      721 tgaacaagct ggccgaaaaa gtcgtggcgg tgatgaatcc ggaagagttg gaaaacttgc
      781 tcgatgatca ctaccttggc gaagcacaaa tcctgacgac cagtgccgaa cataacctgc
      841 tcaaactggc tgaattgcgc gggcggatgt ccccggaaca aaccagccgc tggaacgaga
      901 tcaaacgcgg atttgcgcgg gtcaaagtga tgggcggcaa agatgatgac ccggtggtga
      961 aagtcaccgg tcaaattggg ttggtctctg aacggctcga agatatttgc gctgccctga
     1021 caactttgca ttccgtcacg gcacggcaca ctgagcaatc tgcccgaagc ggtgatctgt
     1081 caacctactt gaccaaactt gaccagacgt tgaatgtgct gcggcgagtc caatca
//
LOCUS       DAOQDO010002409         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_168934_length_1136_cov_2.483811, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002409 DAOQDO010000000
VERSION     DAOQDO010002409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168934_length_1136_cov_2.483811"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKZ53_27620"
     CDS             complement(<1..647)
                     /locus_tag="PKZ53_27620"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HNJ44282.1"
                     /translation="MAKSIDEQLTDLEDDIRKLKIEFDIYFNGGRKRPPYDTRDRVEA
                     MIKRIGDDRSMRFGTRYRYNTIVSRYTAFRELWRRSLQEKEEGRDPRALYEAQAAERA
                     RAAQQKDLDSSFGNEPEFELDLDSAFDSIHEDKAAISFKPVKVSFDDPRHQEEGIKKL
                     YQSVISAKLQCGESIDNTSFDQFYKMIVYNTNKIKATKNCQEVNFSVDVENGQVK"
BASE COUNT          278 a          244 c          291 g          323 t
ORIGIN      
        1 aatttgacct gaccgttttc aacatccacc gaaaagttga cttcctgaca gtttttggtg
       61 gctttgattt tgttggtgtt gtagacgatc attttataaa actggtcaaa cgatgtgtta
      121 tcaatggatt cgccgcattg gagtttggcc gagattacac tctgatagag ttttttgatg
      181 ccttcttcct ggtggcgggg gtcgtcaaac gaaactttga cgggtttgaa tgaaatggcg
      241 gctttgtctt cgtgaattga atcaaaggcg ctatccagat cgagttcaaa ttcaggttcg
      301 ttaccaaaac tggaatcaag gtctttttgt tgggcggcgc gggcgcgttc agccgcctga
      361 gcttcataca gggcgcgtgg gtcgcggcct tcctccttct cctggaggct acgccgccac
      421 aactctcgaa aggccgtata gcgcgagaca attgtgttgt agcggtaccg ggtgccaaag
      481 cgcattgagc ggtcatcgcc aattcgtttg atcatcgctt caacccggtc acgggtgtca
      541 tatggtggac gtttacgacc accattgaag tagatgtcaa attcgatctt gagttttcgg
      601 atgtcgtcct caagatcggt gagttgttcg tcaatactct tggccacaga aacagctcca
      661 aatcaaaatt ccaggcagca aggttaacca gggacagcgg gatgcaggtt gaagaggata
      721 gttgaagttg agtgaacaca cagaccacaa agaatattca atccagaggg attatttcaa
      781 gcagagtttc tggattttga actgaaccgc ttggaaatca agattgaaaa tcaagttgtc
      841 gaggtttttt cttcctacct gaagtggttg tcatttggat tgctgggtaa gcgtcgaaaa
      901 tctgttgttt caatcaaacg ctgaaaaagt tccatccctt tgctgaaaaa agttaaacct
      961 cttctgtaaa aaatcttgac agccgaggag ggggagcctg attgtgtggc aagcaggttt
     1021 tattttggcc acttgcaaca tcgccgggtt ttatgcccag gcaacccaac ccgctttgcg
     1081 ggtcggttat tcttcagtca tcaaggaaca cttcccggat tgagcggttg ctctca
//
LOCUS       DAOQDO010002410         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_169466_length_1134_cov_36.329935, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002410 DAOQDO010000000
VERSION     DAOQDO010002410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169466_length_1134_cov_36.329935"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..>1134
                     /locus_tag="PKZ53_27625"
     CDS             357..>1134
                     /locus_tag="PKZ53_27625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ44283.1"
                     /translation="MLSIDLRSSLRLLRKQPGFSSLIVLTLAVGIAANTALFSVVNAV
                     LLRPLPYRQPASLVMVWDTHQTQGLDHVGPSPGNFLDWQAQASAFDGMATWYESSRTL
                     RGQFEPEQIKVAQASTDFFRVLGMEPLLGRPFTPADTIGTSFNSADQLQSGDRLVVIS
                     RKLWQGRFGGDPQVIGKEISIDGKFWRVVAVMPSGLTLPETEADAWVPWNVRSSYTGM
                     RFPDGPPRDYHFLKVVARLKPGITLEQAQSNLNSVAAGLDQ"
BASE COUNT          246 a          330 c          272 g          286 t
ORIGIN      
        1 gttcttggtt cttggttctt ggttcttggt tcttggttct tggtttcgaa aacccggaac
       61 ccggaacccg gaacccggaa cccggaaccc ggaacccgga acccggaacc cggaacccca
      121 tccttcttcg aaaatcccgc cccaactgtt caaaaccgaa cactcatctc ccgactttgg
      181 gcaccagagc aacatcctta ccaattccta tcgtcaaacc attcattttc aaagatttat
      241 tcatacaatc cctggaacgc cagttgcaca catggcagta cccattgagc tatctgtttg
      301 ctgggttgca gatagaactc agacgcttac ctgctttttc tccagaggaa ctctccatgc
      361 tgtcaattga tcttcggtcc agtcttcgtc tgcttcggaa acaaccagga ttttcaagct
      421 tgatcgtgct caccctggcc gttggaattg ctgctaatac cgcgctgttt agcgtggtca
      481 acgcggtttt gctgcgccca cttccctacc gccagccagc ctcactggtt atggtctggg
      541 acacccatca gacccagggg ctcgaccacg tcggcccctc acccggaaat tttctcgact
      601 ggcaggccca ggcttcggca tttgatggga tggcaacctg gtatgaatca tcacgaacac
      661 ttcgcggaca atttgaacct gagcaaatca aagtggctca ggcttcgact gatttctttc
      721 gagtcttggg aatggaaccc ctgcttggac gcccgttcac accggctgac accatcggca
      781 ccagttttaa ctcggctgat cagttgcaat ccggtgaccg actcgtggtc atcagtcgca
      841 agttatggca gggtcgcttc ggtggggacc cgcaggtaat tggaaaagaa atttcaattg
      901 atggcaaatt ctggcgagtt gtcgcggtaa tgccctcggg actgaccctc cctgaaactg
      961 aggccgatgc ctgggtgccc tggaatgtca gaagttctta tactggaatg cgttttccgg
     1021 atggaccgcc ccgtgattat cactttttaa aggttgtggc tcgactcaaa cctggcatca
     1081 cgcttgaaca ggctcaatcc aaccttaatt cggtggccgc cggcctggac cagc
//
LOCUS       DAOQDO010002411         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_169584_length_1134_cov_2.303058, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002411 DAOQDO010000000
VERSION     DAOQDO010002411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169584_length_1134_cov_2.303058"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1134
                     /locus_tag="PKZ53_27630"
     CDS             <1..>1134
                     /locus_tag="PKZ53_27630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44284.1"
                     /translation="AALIGKTDPTVYLAELDSIEAKTKGPLNPRLWLVRGDLQMQSGK
                     NAEALVWYRKFVAAISQTADQGWEQGCVPQIYYPVSPQFLELYNGTRGPSPYEPVEIY
                     PSLPFHFGSGSQIDNWLIQRFLTLGAWEDAEQEFKRVWAIYQDASSNNQLSGLARQFA
                     LEYAFFLIKYRQPQRGLDLLLNVFLQLDLERNPAVDFSLQENNSRHKAQANGTKAPPS
                     HSEEFRTILLRTSVFSGSEFIRLAYGQFQCHQQIPSLTLAIQRQIQHGRNQARWVLAH
                     ILQLEGKSDEALATELEYLRHAQFNALTITYRSGLTYERFNRLSESATAFEQTLTLPD
                     QPIALPDQDGHQSGSLVFDLSRNRTLEFSIDQKSKVLKRLFEI"
BASE COUNT          309 a          276 c          252 g          297 t
ORIGIN      
        1 ttgcagcctt gattggaaaa actgacccaa ctgtctatct ggcagaactc gactccattg
       61 aagcaaaaac aaaaggtccg ctcaacccac gattatggct ggttcgcggc gatttgcaga
      121 tgcaatcagg caagaacgct gaagcactgg tctggtatcg taagtttgtg gctgctatca
      181 gccaaacagc agatcaaggc tgggaacaag gctgcgttcc tcaaatttat taccccgtga
      241 gccctcaatt tctggagttg tacaatggaa ctcgtggacc cagtccatat gaacccgtag
      301 aaatttatcc cagtttacct tttcatttcg gatccggaag tcagattgat aactggttaa
      361 tccaaagatt tctcacgctg ggggcctggg aagacgccga gcaagaattc aagcgagtat
      421 gggcaatcta tcaggatgca agctcaaata accaattgag tgggctcgcc cgtcaatttg
      481 ccctggaata tgcttttttt ctcatcaagt accgccaacc tcaaagaggg cttgacctgt
      541 tgctcaatgt ctttttacaa cttgatttgg aacgaaaccc agcagttgat ttcagtctcc
      601 aggaaaataa ctcccgtcac aaagcacaag ccaacggaac gaaagctccc ccctctcatt
      661 ctgaggagtt tcggaccatt cttttaagaa cgagtgtatt ttctggatct gagtttatcc
      721 gattggccta tgggcagttt cagtgccatc aacaaatccc gtctcttaca ttggcgatcc
      781 agcgtcagat tcaacacgga cggaatcagg cccggtgggt tctggctcac atcctgcaac
      841 tggaaggaaa gagtgacgag gcgttggcaa ctgaactgga gtacttacgt catgctcaat
      901 tcaatgccct cacgattacc tatcgatcag ggttgaccta tgagcgattt aatcgtctat
      961 cagaatcagc gaccgctttc gaacaaactc tgacactccc tgatcaaccg attgccttac
     1021 ctgatcagga tggccatcaa tcaggttctt tggtatttga tttaagccgc aaccgaaccc
     1081 tggagttttc aattgatcaa aaaagtaaag tcttgaaacg gttgtttgaa attt
//
LOCUS       DAOQDO010002412         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_169802_length_1133_cov_4.426716, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002412 DAOQDO010000000
VERSION     DAOQDO010002412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169802_length_1133_cov_4.426716"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..638
                     /gene="phaR"
                     /locus_tag="PKZ53_27635"
     CDS             192..638
                     /gene="phaR"
                     /locus_tag="PKZ53_27635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNJ44285.1"
                     /translation="MPRKQSESDRVLIKRYGNRRLYNTETGSYVNFQDLIALIQSGRD
                     IQVLDSKTKEDITKVILTQIILEEEKNNKNILPLPFLFQLIRYQEKSVQDFFQNYLSA
                     TFEAYLKTKQEFDRRFRDWIEMSPGSPQMWESFFQPPGGKRKDSEE"
     gene            757..>1133
                     /locus_tag="PKZ53_27640"
     CDS             757..>1133
                     /locus_tag="PKZ53_27640"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ44286.1"
                     /translation="MTVLLVVCLIWLVAWPETSGFAQTFNRSRVKSQAETTFDPFDQC
                     QIAFRVFNHKNGLYQTQVNCIEFDQQGYLWVGTADGAARYNGHSWTAVHLPTQTKSNH
                     IKTMLAASDGSMWFGSASAGLSR"
BASE COUNT          315 a          265 c          265 g          288 t
ORIGIN      
        1 ttaaattgtt tattttcaac tagttaacct ttcaaaatta tgtgaaaaac agagggtgtg
       61 gctgtggttt gcttgaaaaa tgccagaaaa tagctcgcaa gtgcgaaaag tttgtaaagc
      121 actgacaaag ttagagatca cttgagatac cattgacgtc attgctgcac ctgtactaga
      181 ttgcgagcat tatgcctcgc aaacaatctg aatcagaccg ggtcctcatc aaacgttatg
      241 gcaaccgtcg gctctataac accgaaacgg gaagctatgt caattttcag gatttgattg
      301 cattgatcca aagcggacga gacattcagg tgctggattc taaaacaaaa gaagacatca
      361 ccaaagtgat tctcacacaa atcatccttg aagaagaaaa aaacaacaaa aacattctcc
      421 ccctgccgtt tttgttccag ctcatccgct atcaggaaaa atcagtccag gatttctttc
      481 aaaactacct ttcagccacc tttgaagcgt atttaaaaac caaacaggaa tttgaccgcc
      541 ggttccgcga ctggatcgaa atgagccctg gttcacccca gatgtgggaa tcatttttcc
      601 agcctccagg ggggaaacgc aaagattccg aagagtgaaa acctgggtca gtaccacctg
      661 cgtcagtggg tgggtcttta ttttcgattc aacctgaaac ccacccgctg acgcaggtgg
      721 tactgacgaa aagagtccct gcccatgaag cattttgtga ccgttcttct tgttgtgtgt
      781 ctgatctggc tggtggcttg gcctgaaacc agtggatttg cccagacatt caatcggtct
      841 cgggtcaaat cccaggcaga aaccacgttt gatccgtttg atcagtgcca gattgctttt
      901 cgagtgttta atcacaaaaa cggactgtac caaacccagg tcaattgtat tgagtttgat
      961 caacagggat atttatgggt ggggacggct gatggcgcgg cgcgctataa cgggcactcc
     1021 tggacggcag ttcacctgcc gacccaaaca aagtccaatc acatcaagac gatgctggcg
     1081 gcttcggatg gcagcatgtg gtttggctcg gccagtgccg ggctttcacg gtt
//
LOCUS       DAOQDO010002413         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_169846_length_1133_cov_2.849722, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002413 DAOQDO010000000
VERSION     DAOQDO010002413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169846_length_1133_cov_2.849722"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKZ53_27645"
     CDS             complement(<1..816)
                     /locus_tag="PKZ53_27645"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNJ44287.1"
                     /translation="MKHLIVNADDFGMCRGVNTGIVDGYHQGIIRSTTLMANGDDFED
                     AVARAKEATGLGVGCHLVLLGGHPVAPPASVRSLLDAQGMFPNDFGVFTRKLMAGRLK
                     YSEIVTEFRAQIERILNTGIRLTHLDSHKHSHAHPTVLDAVLEVAETYRIPAIRKPFE
                     CFWWAPLATLRAGSKLTLLKQKASRAAMSHYGRTFRSKVARHAIGVPDQFYGFMHTGL
                     LTPELVATLIARLPDGVSELMCHPAHMDESLRQYKTRLKESRVNELETMTSPLV"
     gene            complement(827..>1133)
                     /locus_tag="PKZ53_27650"
     CDS             complement(827..>1133)
                     /locus_tag="PKZ53_27650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44288.1"
                     /translation="VVSETPWVARYYCTQFGRLDLKNEILSDARVQLDTEHPKFVIVQ
                     RGRYYFENIERIHWLTSHATLVKEIYVAGKVAAQVYACGKNQPERFDSYIVQARPVR"
BASE COUNT          272 a          318 c          285 g          258 t
ORIGIN      
        1 taccagaggt gaggtcatgg tttctaattc gttgacccgc gattctttga gccgggtttt
       61 atactggcgc aggctttcat ccatatgtgc cggatgacac atcaactcgc tcacgccatc
      121 aggcaacctg gcaatcaggg tggccaccag ttccggggtc aatagcccgg tgtgcataaa
      181 tccatagaac tgatcaggaa caccaatcgc gtggcgggca accttggatc ggaacgtccg
      241 gccataatgg gacatcgccg ctcgactcgc tttttgtttt aacagggtca actttgaccc
      301 ggcgcgcaac gtcgccagcg gcgcccacca gaagcattca aatggtttgc gaatggccgg
      361 gatgcgatag gtttcagcga cttcgagcac ggcgtccaac acagtcggat gagcgtgcga
      421 gtgcttgtga ctatccaggt gggtcaagcg gataccggtg ttgaggatgc gttcaatttg
      481 ggcccgaaac tcagtgacaa tctcactata tttcaaccgg ccagccatca actttcgggt
      541 aaagacgccg aaatcatttg gaaacatgcc ctgggcatca agcaaactgc gaacggacgc
      601 gggcggagca accgggtgac cgcccaacaa caccaggtga cacccgacac caagaccagt
      661 tgcctctttt gctcgggcaa ccgcatcttc aaaatcatcg ccattcgcca tcaaagtcgt
      721 gctgcggata atcccctgat ggtagccatc tacaatacct gtgttgacgc cccggcacat
      781 gccaaagtca tcagcgttca caattaaatg cttcaatctt ccccctttag cgcacgggcc
      841 tggcctgcac aatatatgaa tcaaatcgct caggctgatt ttttccacag gcgtaaacct
      901 gggcggctac cttgccggcc acatatatct ccttgacgag ggtggcgtgg ctggtaagcc
      961 agtggatgcg ctcaatattt tcaaaatagt atcttccgcg ttgcacgatc acaaattttg
     1021 gatgctccgt atcaagttga accctggcat ccgacaagat ttcgtttttt aaatcgagtc
     1081 gcccaaactg agtacaatag tagcgggcga cccacggggt ttcgctcacc acc
//
LOCUS       DAOQDO010002414         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_169899_length_1133_cov_2.370130, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002414 DAOQDO010000000
VERSION     DAOQDO010002414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169899_length_1133_cov_2.370130"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKZ53_27655"
     CDS             <1..903
                     /locus_tag="PKZ53_27655"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ44289.1"
                     /translation="RITKFEAVLCQQADLVIALECPVPWIPALSQVKTPPTLIQIGVD
                     PLFQAIPMRSFPADVALAGDPAATLQKLTSLLGQCEHLEHRAQRRETIAVEHTRMVSA
                     CRTAAAGDSSKSVITKRFLSWCIGQVVDDETVIFNEYNLDPQLVSRRLTDSWFENSVA
                     SGLGWSLGAALGAQLARPELTMVVTLGDGSYLFNTPLSAHYVAAAYNLPIIIVVFNDS
                     AWTTIKKSYLGTTPEPWGVKNNAFPLCNFDVQIQFEKLAESCGGVGLTVSEPGDVIET
                     LKQAFHISRQERRHVLVNVLCERD"
     gene            complement(923..>1133)
                     /locus_tag="PKZ53_27660"
     CDS             complement(923..>1133)
                     /locus_tag="PKZ53_27660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNJ44290.1"
                     /translation="GKSGWVPKHTFEADGQSGQTVMDYTARELTAHDGENLLVHKIEF
                     GWAWCTNRDEVSGWIPESCLELITT"
BASE COUNT          239 a          300 c          308 g          286 t
ORIGIN      
        1 cgcattacga aatttgaggc ggttttgtgc caacaggctg atctggtcat tgcgctggaa
       61 tgtcccgttc cctggatccc ggccctgtcc caggtcaaaa ctccgccaac gttgatccag
      121 attggggtgg atccgctctt tcaagccatt cctatgcgca gcttcccagc cgatgtggct
      181 ctggccggag acccggcagc caccttgcag aaactgacca gtttgctcgg ccagtgtgag
      241 catttggaac atcgcgccca acgccgtgaa accattgccg tggaacacac ccggatggtg
      301 tcagcctgtc ggacggcagc ggctggtgat agctcgaaat cagtgattac caagcggttt
      361 ctgtcgtggt gtattgggca ggtggtggac gatgaaacgg tcatcttcaa cgaatacaac
      421 ctggatccac agcttgtttc gcgccggttg acagatagct ggtttgaaaa ttcagtggcc
      481 agcggtctgg gttggtcgct tggggccgca ctcggcgcgc aactagccag acctgaactc
      541 acaatggttg ttaccctggg tgacgggagc tatttattca acactccgct ttcggcgcac
      601 tatgtagccg ccgcctacaa tttgccgatt attattgtgg tctttaatga cagcgcctgg
      661 actaccatca agaaatccta tttaggtacc acgcctgaac cctggggtgt caaaaataac
      721 gccttcccgc tgtgcaattt tgatgtgcaa atccagtttg aaaaactggc tgagtcgtgt
      781 ggtggcgttg gattgacggt ttctgaacca ggagatgtga tcgaaaccct caaacaggca
      841 tttcacattt cccgtcagga gcgacgacac gtcctggtca atgtgctttg cgagcgggat
      901 tgaaacgtaa aaggttccat cttcaggtgg ttatcagttc aagacagctt tctggaatcc
      961 agcctgacac ttcatcgcgg ttggtgcacc acgcccagcc gaattcaatc ttgtgaacaa
     1021 gcaggttttc tccgtcgtgg gccgtcagtt cacgggcggt gtaatccatc acggtctggc
     1081 ctgattgccc gtcggcttcg aaggtatgtt tgggtaccca accgcttttt ccg
//
LOCUS       DAOQDO010002415         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_169905_length_1133_cov_2.325603, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002415 DAOQDO010000000
VERSION     DAOQDO010002415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169905_length_1133_cov_2.325603"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1133
                     /locus_tag="PKZ53_27665"
     CDS             <1..>1133
                     /locus_tag="PKZ53_27665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44291.1"
                     /translation="SAGISVVAAGTCGPALPRPDGSAEPNQTVGDGGQNHLRVWRDGG
                     ERDKVRQVTQEELDLLKAAAISRWTTADIPAEVLEKMRNITISLGDLPIGIIAINDPQ
                     SIVVDYSAAGYGWFVDSSPLGDEEFQRVGSSQLYVARPNTPAFRKADLLTVIMRQLGH
                     IAGLKALRGDRFKDQLMANMFFESIRRLPDASFDDLRRLTTEEAKVYAPASASPTQSP
                     RLALGSSSWTIGQMTSVLPVFLKKTTDASPTFPARFGEFTPELKKAVRVIPATSDITA
                     ATPSSLPPALPDDLEPQKGTQRNRPPQSRAVINPLAGETVSQTIGTLPGGESVTIRFN
                     VTVNNPLPTPQISPSQPAVTQVLNQGTVSGGNFSNVLTDDPDIA"
BASE COUNT          265 a          347 c          292 g          229 t
ORIGIN      
        1 agtgccggga ttagcgtggt ggcggcagga acctgcggac cggcactccc gagacctgac
       61 ggcagtgccg aacccaatca aaccgttggc gatggcggcc aaaaccatct gcgcgtctgg
      121 cgtgatggcg gcgagcgcga caaagtcaga caggtcaccc aggaagaact cgacctgctc
      181 aaggcggctg cgatttcccg ctggaccacg gctgatattc cggctgaagt ccttgagaaa
      241 atgcgaaaca tcaccatttc gctgggtgac ttgccaattg ggattattgc tatcaacgac
      301 ccgcaatcca tcgtggttga ttattcggcg gctggatacg gctggtttgt agattcgtca
      361 ccgctcggcg acgaagaatt ccagcgcgtt ggttcgtcac aattgtatgt tgcccgcccc
      421 aacaccccag ccttccgcaa ggccgatctg ttaacggtca tcatgcgaca actgggccac
      481 atcgctggat tgaaagcgct ccgtggagac cggtttaaag atcagttgat ggccaatatg
      541 ttctttgaat ccattcgccg gttgcccgac gcgtcctttg acgacctgcg ccgtctgacc
      601 actgaagaag ccaaagtcta tgcacctgcc tcagcatcac caacccaatc cccacggctg
      661 gccctgggaa gttcaagctg gaccatcggc cagatgacat cagtcctccc cgtctttttg
      721 aaaaagacaa ccgatgcgtc accaaccttc ccggctcgat ttggagaatt caccccggaa
      781 ctgaaaaaag cggtgcgcgt cattccagcc acgtcagaca tcaccgcggc aaccccttcg
      841 tcacttccac cggcattacc tgatgacctg gagcctcaaa aaggcacaca gcggaaccga
      901 cctccccagt ccagagcggt gatcaatccg ctggcgggtg aaaccgtcag ccagacgata
      961 ggcacacttc caggcggtga atcagtcacc atccggttta acgtcacggt gaacaaccca
     1021 cttccgacac ctcagatttc gccatcccag ccggcggtaa cgcaggttct caatcaaggc
     1081 acggtttctg ggggaaattt ctcaaatgtg ttgaccgatg acccagatat tgc
//
LOCUS       DAOQDO010002416         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_170736_length_1130_cov_3.148837, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002416 DAOQDO010000000
VERSION     DAOQDO010002416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170736_length_1130_cov_3.148837"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKZ53_27670"
     CDS             <1..323
                     /locus_tag="PKZ53_27670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44292.1"
                     /translation="IIYVPTAQVSHNVDAGQVSFDWLFRRAFLYGRGRARMDSEPALL
                     QIFNVPVRHWLRLPLAWIQCGLSRFQTPARRFQAGVRYHRLRGEVYEYRQLASERNSE
                     VAKS"
     gene            complement(394..729)
                     /locus_tag="PKZ53_27675"
                     /pseudo
     CDS             complement(394..729)
                     /locus_tag="PKZ53_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
BASE COUNT          285 a          280 c          279 g          286 t
ORIGIN      
        1 agattatcta tgttccgaca gcgcaggtaa gccacaatgt ggatgcaggc caggtttcat
       61 ttgactggtt gtttcgtcgg gcctttttat atgggcgggg ccgggcgcgg atggactctg
      121 aaccagccct gctgcaaatc ttcaatgttc cagtgcggca ctggctcagg cttccgctgg
      181 cctggattca atgtgggctg agtcggtttc agactcccgc ccggcgcttt caagccggtg
      241 tcaggtatca tcggctccgt ggcgaagttt acgaatatcg ccaattggca agtgaacgaa
      301 atagcgaagt agcaaaatcc tgaaagacaa agagttgagg gattgataac acccaggttg
      361 aaaatcaatg ttgagctact tcgctcgttc gctttatttg atgcccagca attcaacttc
      421 aaaaatcaga gttgaattcg gtggaatggg gccaatcccc cggcttccat aaccaagctc
      481 aggcggaacc gtcagtttgc gtttgccacc aattttcatg gtcgcaacac cctgatccca
      541 ccctttgatg acctgtccca cacccaaaat aaactcaaat gggcggttgc gatctttcga
      601 actgtcaaat ttgacgccat tggtgagcac gccggtatag tgaaccacca ctgtgtcacc
      661 gatttttggt gacgcgcctg acccaaccac gatttcttca atgcctaaag ttgcactcat
      721 gcgtttgttc tgtccttcaa ataataaaga taaagaaatt caggtgttta gggacccgga
      781 agaaaataaa atcccaatga aatcgggttc agggttcagg gttcaggggt ttaggggatg
      841 aggtgactga tcccactcaa gattgaggac tggggaacca gcagttaccg ttcaaactgg
      901 attatctggt tctgaaccct gaaccctgaa ccctgaaggt atgatatttc catttgactc
      961 ccttaaggta cctgacattt caaagcggat ggtttctatc aaaggaaatc ttatttggct
     1021 agccggtcat ggcgaaggcc gctgacccgg catactttga agcgcgaacc cgaggcgctc
     1081 aaccccccgg tcaatttcct tttcatcaac tgcggcaaag ccaaggatca
//
LOCUS       DAOQDO010002417         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_170786_length_1130_cov_2.507907, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002417 DAOQDO010000000
VERSION     DAOQDO010002417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170786_length_1130_cov_2.507907"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKZ53_27680"
     CDS             <1..450
                     /locus_tag="PKZ53_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNJ44293.1"
                     /translation="GLVVDLHHLRLETLQLAAGAFLLRKIYREMFHWGESKHLRLAIV
                     LDEAHRLAKDITLPKIMKEGRKFGVAVIVASQGLADYHPDVVGNAGTKVIFRTNYPMS
                     KKVAGFLRPPKNLDLSSVIEQLEVGEAFIQTPDMITCARVRMRNLNE"
     gene            complement(549..>1130)
                     /locus_tag="PKZ53_27685"
     CDS             complement(549..>1130)
                     /locus_tag="PKZ53_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44294.1"
                     /translation="ALKQIQRPELLHIATHGQFLDARAVLPASEDEKRPGITVDVEKL
                     KVENPLLRSWLFFAGANHGGSDENDGTLTALEAAQLDLWGTKLVVLSACETGVGEAKT
                     GDGVYGLRRALVLAGSESQLMSLWSVSDRATRELMVEYYTRLKAGEGRSDALRRVQLK
                     MLKDPKRRHPFYWASFIQSGEWANLAGTRKLRS"
BASE COUNT          251 a          306 c          297 g          276 t
ORIGIN      
        1 ggacttgtcg ttgatctgca tcacttgcga cttgaaaccc tgcaactggc cgctggggca
       61 tttttgctcc gtaagattta ccgtgagatg tttcactggg gtgaatcaaa acatctacgg
      121 ttggcgattg tcctggatga agcgcatcgc ctcgccaaag acatcacctt gccaaaaatc
      181 atgaaagaag gtcgtaaatt tggggtggct gtcattgttg ccagtcaggg gctggcggac
      241 tatcacccag atgtggtcgg aaatgcgggg acaaaagtga tttttcgaac gaattatccg
      301 atgtccaaaa aagtggccgg cttcctcaga ccgccgaaga atctggatct gtcatcagtt
      361 atcgagcaac tagaggttgg cgaagctttc atccaaacac ctgatatgat cacctgtgct
      421 cgggtcagaa tgcgcaatct caacgagtaa atatttcagt tcagagtaac accttcaggc
      481 gtgaggttta gcaagtggga cgaccgatga aaccttctcg tctcaagacg atactctgaa
      541 cctttttctc aagatctcaa ttttcgggtt ccggccaggt ttgcccattc tccggattgg
      601 ataaaggacg cccagtaaaa cggatggcgg cgtttggggt ctttcagcat cttgagttgc
      661 acccggcgga gcgcatcgct gcggccttct ccggctttga gtcgggtgta atactcaacc
      721 atcaattccc gcgttgcacg gtctgatacc gaccagagac tcatcagttg cgattcgctc
      781 ccggccagca ccagcgcgcg ccggagtcca tacacgccgt ctccggtttt ggcttcgccg
      841 acacccgttt cacaggcgga gagcacaacc agtttggtgc cccacaaatc cagttgtgcc
      901 gcctccagcg ccgtcaatgt accgtcgttt tcgtcagacc ctccgtggtt ggcgcccgca
      961 aaaaacagcc acgaccgcag aagcgggttt tcaactttga gtttttcgac gtcaaccgtg
     1021 ataccaggtc gtttttcatc ttccgaggct ggaagcacgg ccctcgcgtc caaaaactgc
     1081 ccgtgggtcg caatgtggag cagttccggt cgctggattt gcttgagcgc
//
LOCUS       DAOQDO010002418         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_171331_length_1128_cov_3.424045, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002418 DAOQDO010000000
VERSION     DAOQDO010002418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171331_length_1128_cov_3.424045"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /locus_tag="PKZ53_27690"
     CDS             <1..>1128
                     /locus_tag="PKZ53_27690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44295.1"
                     /translation="LGADHPNVAISLSKLALLYCEEGDFARAEPLYLRALAINEKALG
                     ADHPQVATSLNDLAVLYQSKGDSAKAIQYRTLSNNTTERDLIHNLGSGSELQKALYLK
                     QTELRTDLSISLHMQAAPQDPEAAKAALTVILRRKGRALDAMTSAIEVLRRQQTPEHQ
                     KQLELYARLNSQISVLTLRGPGKQKAEDYLRYVGELKNQKEQVEHEISRLSAEFKTQV
                     TPIHLENIQKMIPPDAQLVEYAVYRPFDSKTRRFGTPRLAVYLLNNTGEIHFADLGET
                     HPIEAAVDDFRRLVRETSSQGRSIRLGSLPKTSPVDLKTAGQTLEKLIFEPVRKLLGS
                     TTRVLLSPDGILNLIPFDALVDDQGTYLVETFEFSYLTSGR"
BASE COUNT          288 a          296 c          287 g          257 t
ORIGIN      
        1 cgttgggcgc cgatcaccca aatgttgcga tcagcctgag taaactggcg ttgctctatt
       61 gtgaagaggg tgattttgcc cgagcggaac cgctctatct gcgggcactg gccatcaatg
      121 aaaaggcatt aggggctgat catccacagg ttgcgaccag cctcaatgac ctggcggtgc
      181 tttaccagag taaaggggat tctgcaaagg cgattcagta tcgaaccctg agcaacaaca
      241 cgaccgagcg tgacctgatc cacaacctgg gttccgggtc cgagctccaa aaggcgctct
      301 atctcaaaca gacagaactt cgaaccgatt tgtccatctc acttcatatg caggccgcac
      361 ctcaagatcc cgaagccgca aaggcggccc tgactgttat tctgcggcgg aaagggcggg
      421 cacttgacgc aatgacctcg gccattgagg tcctgaggcg gcaacaaacc cctgaacatc
      481 aaaagcagct tgaactgtat gcccggttga acagtcaaat ttcagtctta accctgagag
      541 gtccaggaaa gcaaaaggca gaagactatc tgcggtatgt gggcgagctc aaaaatcaga
      601 aagaacaggt ggaacatgaa atcagtcgtc tgagcgctga atttaaaacc caggtgaccc
      661 caattcacct ggagaatatt caaaaaatga tcccgccgga tgctcaactg gtcgaatatg
      721 ccgtgtatcg tccctttgat tccaaaaccc gacggtttgg aacccctcga ttggctgtct
      781 acctgctgaa caacaccggt gagattcatt ttgctgattt gggtgaaacg cacccaattg
      841 aagctgccgt ggatgatttc cggcgccttg tgcgtgaaac atccagccag ggtcgttcaa
      901 ttcgacttgg ttcccttccg aagaccagtc cggtagacct caaaacggcg ggacaaaccc
      961 tcgaaaaact catttttgaa cctgtccgga agctgcttgg ctcgaccacc agggtgctgc
     1021 tttctccgga tgggattttg aatctcattc cttttgatgc tttggtggat gaccagggca
     1081 catatctggt cgaaaccttt gaattctctt atttgaccag cggacgtg
//
LOCUS       DAOQDO010002419         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_171350_length_1128_cov_2.976701, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002419 DAOQDO010000000
VERSION     DAOQDO010002419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171350_length_1128_cov_2.976701"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /locus_tag="PKZ53_27695"
     CDS             complement(<1..>1128)
                     /locus_tag="PKZ53_27695"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNJ44296.1"
                     /translation="GVNAVRIWERVGAVYVKVAAAGEAPSENIPEETITLLRSGLPSR
                     TVQQRPEGTHASLYRVFAPVFTGSRLSGAVELIELLDDTPSIAAQSQRMSIWIALVAV
                     LVITLGTLTLFDRLIYRPIGQLLKAMNEVEAGNLAVEITGTSSDELGILTRNFNGMIQ
                     RVRTMTEEREAQKLLLQEQVNEATQELSERNQQLESANRELWQLSRRLSEMERLAVAG
                     QTAAQFAHEVGTPLNLISGHVQLLQNNLKQDPKALSRLETINIQIARIERIVRSMLDR
                     TRSEATPFAPVDLDTLLNRIIDVITPTLHQRDIHMVTEFASSLPLINGNSDRLQQVFF
                     NLINNAMDAMAEGGSLTIKTECKTSDCGEPLQVIVSVVDTGC"
BASE COUNT          255 a          276 c          313 g          284 t
ORIGIN      
        1 acacccagta tcaaccacag aaacaatcac ttgcaatggt tcgccgcagt ctgaggtctt
       61 gcattcggtt ttgatcgtca acgagccacc ttctgccatt gcatccatcg cattattgat
      121 caggttgaaa aagacctgct ggagccgatc agaattgcca ttgatgagcg gaagtgaaga
      181 agcaaattca gtcaccatgt gaatgtcacg ctggtgcagg gtgggggtaa tcacatcaat
      241 gattcggttg agtagggtgt caagatcaac tggtgcaaac ggtgtggctt ctgagcgtgt
      301 ccggtcgagc attgatcgca caatgcgctc aatccgcgca atctgaatat tgatggtttc
      361 aagccgtgaa agggcttttg gatcttgttt aaggttattt tgcagaagtt gaacgtgccc
      421 gctgatcagg ttgagcggtg ttccgacttc gtgggcaaac tgggcggcgg tttgaccagc
      481 caccgccaga cgctccattt ctgagagccg acgtgaaagc tgccagagtt cgcggttggc
      541 tgattccagt tgctggttgc gctcagaaag ttcctgcgtt gcttcgttta cctgttcctg
      601 gagcaatagt ttttgagctt cgcgttcctc ggtcatggtt cgcacccgtt gaatcatgcc
      661 gttaaaattt cgagtgagta tgccaagttc gtcactgctg gtgcctgtga tttcaaccgc
      721 cagattgcca gcttcgacct cattcattgc ctttaaaagt tgcccaatcg ggcgataaat
      781 caggcggtca aagagcgtga gtgttcccag ggtaatcacc aggaccgcaa ccagagcaat
      841 ccagattgac attcgttgtg attgagccgc aattgacggc gtgtcgtcga gcaattcaat
      901 cagttcgacg gcaccactga gtcgcgaacc tgtaaacacc ggcgcaaaca cacgatagag
      961 cgaggcatgg gtgccttcgg gacgttgttg aacggttcgg gaggggagcc cgctccgcag
     1021 taaggtaatg gtttcttcag ggatattttc ggatggggct tcgcccgctg cggccacttt
     1081 gacatagacg gcgccaacgc gttcccaaat gcgaacggca ttgactcc
//
LOCUS       DAOQDO010002420         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_171389_length_1128_cov_2.450140, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002420 DAOQDO010000000
VERSION     DAOQDO010002420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171389_length_1128_cov_2.450140"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /locus_tag="PKZ53_27700"
     CDS             complement(<1..>1128)
                     /locus_tag="PKZ53_27700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ44297.1"
                     /translation="RVAIKILNPELVSNPAAMERFRREALASGRLKHPNAITIYDYGT
                     TDRGEAFIVMEYLEGCSLNQEIQTVGPMDSTRVAWILAQACDAVHAAHQEGIIHRDLK
                     PANIMLEKLRTGEVVKVLDFGIAKLATSSNPNIMNLTGTGIIGTPQYMSPEQCQAHNV
                     DARTDVYSLGIIAYEMLTGTLPFDGPAPLAIVIAQVKQPPPKLRDRRAEIPEAAEAVV
                     MRALEKDPAKRQQTAEEFARDFRSLIRQLSTSRSTSQATGNISPPRESRPGGLTSHAV
                     PPIKGDRITALETIIRPGVPAAGAATVTPGTEYSGALPLSNLPPRPVRELVSLPTYAS
                     MERFVGRTQELSLLRAAWQRIQQGRGGRPILIFGEPGIGKTR"
BASE COUNT          238 a          302 c          331 g          257 t
ORIGIN      
        1 gcgggtcttt ccgatcccag gttccccaaa gatcaaaatc ggtcggccac cacgaccttg
       61 ctggatacgt tgccaggcgg cgcgtaacag gctcaactcc tgggtccgtc caacaaatcg
      121 ctccatcgag gcataggttg gcaggctcac cagttcacgc accggacgcg gtggtaaatt
      181 gctgagtggt aatgctcctg aatattcagt tcctggagtt acagtggcgg cgcctgccgc
      241 cggaacccca ggtcgaatga tggtttcaag cgctgtaatc cgatcacctt taatcggcgg
      301 gacggcatga ctggtgagtc cgcctggacg actttcacga ggaggggaaa tattgccggt
      361 tgcctgggag gtcgagcggg aggtggaaag ttgacggatc agggatcgga aatcgcgggc
      421 aaattcttca gccgtttgct gacgtttggc aggatctttt tccagcgcgc gcatcacgac
      481 agcctcagcc gcttctggaa tttccgcccg acgatcacgc aactttggtg gcggctgttt
      541 gacctgggca attacgatgg ccagtggggc ggggccatca aacgggaggg ttcctgtcag
      601 catttcatag gcaatgatgc cgagcgaata gacatcggtg cgggcatcaa cattgtgtgc
      661 ctgacattgc tcgggagaca tatactgggg cgtgccgata atcccggtcc cggtcaaatt
      721 cataatattt ggatttgaac tggtggcgag tttggcaatt ccaaagtcaa ggaccttgac
      781 cacttccccg gtgcgcagct tttccagcat gatgttggcg ggtttgagat cccggtgaat
      841 gatgccttcc tggtgcgcag catggacagc atcacacgcc tgggccagaa tccaggcgac
      901 acgggtagag tccatcgggc ccacggtttg aatttcctga ttcaagctac agccttccag
      961 gtactccatc acaataaagg cttcgccccg atccgtcgtt ccataatcat aaatggtaat
     1021 ggcgtttggg tgtttgagcc gccccgaagc cagtgcctca cgacgaaatc gttccatggc
     1081 cgccgggttt gaaaccagct ccgggttcag gattttgatg gccacccg
//
LOCUS       DAOQDO010002421         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_171659_length_1127_cov_3.405784, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002421 DAOQDO010000000
VERSION     DAOQDO010002421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171659_length_1127_cov_3.405784"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..>1127
                     /locus_tag="PKZ53_27705"
     CDS             206..>1127
                     /locus_tag="PKZ53_27705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44298.1"
                     /translation="MSCLLLAGVQYRHEPRLQAATGAQVRVASKSTQRSPHVPALSQE
                     GPIAESALQQMQWLIEAKAKRTPVQRKIDSNLLFLLETNQGTNQTRIPTQMLKPAVQT
                     GTDGRVEVEICSELSDAVLQTITAIGGHISYKSPKYRTIQARLPLASLEKIAAFPEVY
                     FIQPRQEAFTRRAQSPTSSQIQTQPVTDDSFEARSKRVQSFLTSAIPQLVQNQNGELR
                     QPSKVNTTEADVAHRARLARTTFNADGTGIKVGVLSDSASFAQLRSLQRTGDLPANVT
                     ILPGQEGEGSDEGLAMLEIIHDLAPGAQLFF"
BASE COUNT          314 a          295 c          260 g          258 t
ORIGIN      
        1 agagttgggt taagtcaccc acactctata ttgcacgtca cctgacctgt attactccaa
       61 aaaaccaaaa tcagaaactc aattggaacc ccgatgtatg tcagttaaga accaaaatcc
      121 atgccaggca caaacggata aatcttataa aataaagatt tttcagagtt tatactccaa
      181 atcccgattc tttctctttc tattgatgag ctgcctcttg cttgccggag tccagtatcg
      241 ccatgaaccc aggttgcagg cagcaactgg tgctcaggtg cgagttgctt caaaaagtac
      301 tcaacgaagt ccgcacgttc cagccttgag ccaggaaggc ccgattgctg aatcagcctt
      361 acagcagatg cagtggttga ttgaggccaa ggcaaaacga acaccagttc aacggaaaat
      421 tgattcaaac ttgctgtttt tgcttgaaac gaatcaggga acgaatcaaa cccggattcc
      481 aactcagatg ctgaaaccag cggttcaaac tggcacggat ggccgggtcg aagtggaaat
      541 ctgtagcgaa ctctctgacg ccgtgctgca gacaattacc gcgattggtg ggcacatcag
      601 ctataaatct cccaaataca gaacgatcca ggccagactt ccgcttgcct ctctggaaaa
      661 aattgccgcc ttcccggaag tgtattttat ccagccccgg caggaagctt tcacccggcg
      721 ggctcagtct ccaacatcca gccaaattca aacccaacca gtaactgatg atagttttga
      781 agctcgatcc aaaagagttc agtcattttt aaccagtgcg attccacaac ttgttcaaaa
      841 tcagaatgga gagcttcggc agccatcgaa ggtcaacacc accgaagccg atgttgccca
      901 tcgggccaga ctggcccgaa cgacttttaa cgctgatggg acggggatca aagttggtgt
      961 gttgtcagac agcgccagtt ttgcccagtt gcgtagcctc caacgaacag gcgatttacc
     1021 tgccaacgtg accattttgc ccggccagga aggcgaaggg tcggacgaag gtctggcgat
     1081 gcttgaaatc atccacgacc tggcgcctgg agcacaactt tttttcg
//
LOCUS       DAOQDO010002422         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172015_length_1126_cov_2.502334, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002422 DAOQDO010000000
VERSION     DAOQDO010002422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172015_length_1126_cov_2.502334"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1126
                     /locus_tag="PKZ53_27710"
     CDS             <1..>1126
                     /locus_tag="PKZ53_27710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ44299.1"
                     /translation="VAPVAHRVGEVLLDALAPVLSEFFSFESLVLLLPDEETEGLRVR
                     YQRGTAIPTLPEFHSDQLSEVERCLIHNGINHYRLFPFTIASQQTGFLMLLGVQPLAM
                     SQSDSTFLQALHRQISVAITQVHRAERAHQTETDLKQRLDQMQSLLEVTNTTTSHLEL
                     RALFKAIAESIHRMIRCEGAALTIYDAQSGGLRAFALEPRPDFQPKAPLFIQEGDLLP
                     IDDFPITAAFRTRQTKLVSLDFLQQSKSPFVQKALALGFKSGCAAPLISQGKAIGTVS
                     VVRLTEGSFTAADAELLTKIANQIAIAVDNALNFERARQAEQQVTRERDLWRFLLDVN
                     NAVVSHLDLKDLVKTIASQLKHLMPVEFSGISLLDSEGTHL"
BASE COUNT          256 a          311 c          285 g          274 t
ORIGIN      
        1 ttgtggcacc cgttgcacat cgggttggtg aggtgctcct ggatgcgctc gcgccagtac
       61 tgagcgagtt cttttccttt gaaagcctcg tcctgctcct ccctgatgag gaaacggagg
      121 ggttgagagt tcgataccag cgtggaacgg caatcccaac gctgcctgaa ttccactctg
      181 accagttgtc agaggtggaa cgctgcctga tccacaatgg aatcaatcat taccggctgt
      241 ttcccttcac aattgcatcc caacaaacgg gcttcctgat gctgctcggg gttcaaccgt
      301 tggcgatgag tcagtctgat tcaacgtttc tgcaggctct ccatcggcaa atctcagttg
      361 ccattaccca ggtccaccgg gctgaaaggg cgcaccaaac cgaaacggac ctcaaacaac
      421 gacttgatca aatgcagtcg ttgcttgaag ttaccaatac cacgacatca caccttgaac
      481 tacgggcgct ctttaaagct attgccgaat ccattcaccg gatgatccgc tgtgaaggtg
      541 cggccctgac catttacgac gcccaaagcg gcggcttacg ggcctttgcc ctggagccac
      601 gcccagactt tcagcccaaa gcgccgttgt ttatccagga aggtgacctg ttgccgatag
      661 atgattttcc gattacagca gcatttcgaa cccggcaaac caaactcgtt tcgcttgact
      721 tcctgcagca gtcaaagtct ccctttgtcc aaaaagcact tgccctggga tttaagtcag
      781 gctgtgcggc accgctgatc tctcagggca aagcgattgg gacggtgagt gtggttcgat
      841 tgactgaagg ttcgttcaca gcggctgatg ccgagttatt gactaaaatt gccaaccaaa
      901 ttgcgattgc ggttgataac gccctcaatt ttgagcgtgc gcgccaggct gagcaacagg
      961 tcacacggga acgggacctc tggcggtttt tactcgatgt caacaatgcc gtcgtgtcac
     1021 atctggacct gaaagacctg gtgaagacga ttgcttctca attgaaacat ctcatgccgg
     1081 tcgagttttc tggcatttcg ctccttgaca gtgaaggaac tcacct
//
LOCUS       DAOQDO010002423         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172266_length_1125_cov_4.089720, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002423 DAOQDO010000000
VERSION     DAOQDO010002423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172266_length_1125_cov_4.089720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..>1125)
                     /locus_tag="PKZ53_27715"
     CDS             complement(19..>1125)
                     /locus_tag="PKZ53_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ44300.1"
                     /translation="SLDWHAARLRHKLEAVVAACLPDQSPVGVYLSGGLDSSCITALA
                     RKLHSDPVHTYSIHFGSETPNELEFSSAVAEHCQTRHHIIEITSDQMWHLLPQTLAMM
                     DDPVGDPLTIPNLILGQTARQDVGVILNGEGGDPCFGGPKNQPMLLNQLYKPASGHKP
                     EAQLAAYLASFQKCSTDLSTLLLPEVWKTASQSPSVFANDLFGSAHYLNRLLMINIKF
                     KGADHILTKVNNITTALGLVGHSPLFDHRVVEMSLDIPPEFKLDGAEEKAVLKAAVAD
                     LIPKSILDRPKSGMMVPVQYWFREKWHIRARALLMSRKARIRNYLNPQVIQQWLDYQG
                     DVWHRYGVKLWLLVTLEIWLQVNESTDSALKGRA"
BASE COUNT          270 a          279 c          292 g          284 t
ORIGIN      
        1 gtgtcgtggg tacacgactc aagctcggcc cttcagggcc gagtctgttg actcattgac
       61 ttgcagccag atttcaagcg tgaccagcag ccataatttc accccatagc ggtgccagac
      121 atcaccctga taatcgagcc attgttgaat tacctgtgga ttcaaatagt tacgaattct
      181 tgcctttcga ctcatgagca gtgcccgcgc ccggatgtgc catttctcgc gaaaccagta
      241 ttgaaccggc accatcattc cacttttggg gcgatcaagg atgcttttcg ggatcaaatc
      301 agccaccgcc gcttttaaca ccgctttttc ttcagctccg tccagtttga attccggcgg
      361 gatatcaaga ctcatttcaa ccacgcggtg atcaaaaagt ggagagtgtc cgacaagccc
      421 gagagctgtc gtgatgttgt tgactttggt gaggatatgg tcagcacctt taaacttgat
      481 gttgatcatc agcaaccggt tgagatagtg cgctgaacca aacaaatcat tggcaaaaac
      541 agacggtgac tggctggcag ttttccaaac ctctggtaac aataaggttg acaggtcggt
      601 tgagcacttc tggaatgaag ccagataggc cgcaagctgg gcttcaggtt tgtgtccgct
      661 ggctggtttg tacagttgat tgagtaacat cggctggttt tttgggccgc caaagcaggg
      721 gtcaccgcct tcaccattta aaatgacacc cacatcctgc cgtgctgtct gccccaggat
      781 cagatttggg attgtcagtg gatctccaac cgggtcgtcc atcatggcca gtgtttgtgg
      841 cagcaggtgc cacatctgat cagatgtgat ttcaatgatg tggtgccgag tctgacagtg
      901 ttcagctacg gctgatgaaa attcaagctc gttgggtgtc tcggatccaa aatgaatgga
      961 ataggtatga accgggtctg aatgaagttt tctggccagt gccgtgatac aactcgaatc
     1021 caacccaccg gaaagatata ccccgaccgg cgattgatca ggcaaacagg ctgcaaccac
     1081 ggcttccagc ttgtggcgta atcgagcggc atgccagtca agtga
//
LOCUS       DAOQDO010002424         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172296_length_1125_cov_3.081308, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002424 DAOQDO010000000
VERSION     DAOQDO010002424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172296_length_1125_cov_3.081308"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1067
                     /locus_tag="PKZ53_27720"
     CDS             402..1067
                     /locus_tag="PKZ53_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ44301.1"
                     /translation="MIVLRNLEKSFDHGHTRHFVLRRINAEIKPGEFISIMGPSGAGK
                     TTLLNILGMHDSAWTGEYLLNEQPIHKLSKKERFEIQKKTFGFVFQSYHLLDNLTVYE
                     NLEIPLSYRNIKRKECESMVCDVLDRFQIVGKKDLYPNQLSGGQQQLVAIARAIIASP
                     KVILADEPTGNLHSKQAEEIMELFKRLNDEGTTIIQVTHSERNASFGNRVINIFDGWI
                     AAE"
BASE COUNT          315 a          281 c          234 g          295 t
ORIGIN      
        1 ctttgaccat gtcgaagcct gatccaaacc agtcgcttat accagcttgc agtgaacctt
       61 tcgtatcagc aaggagtcaa atagttgaat caactaagct tagtaaccaa ctgatattgt
      121 cttcattctt cattcttcat tcttcattct tcattcaaag ctgttgtctt cattcttcat
      181 tcttcattct tcactcttca ttcttcactc ttcattcccc attctccatt caaaatggct
      241 aaatggcaga tatttcaaaa tcttggaata cagtttgcac tgaggatgtc tgtccacttt
      301 gagtcaattc aaagatctca caaaaattga agagttcaga gttctgattg aaaccggaac
      361 tctgaactat tttttgaagc ctgcttttga ggaaacccat catgatcgtg ttgcgcaacc
      421 tggaaaaatc ctttgatcac ggccataccc ggcattttgt cctgcggcga atcaacgccg
      481 agatcaaacc tggcgaattt atttcgatta tggggccctc aggtgcgggg aaaacgacct
      541 tgctcaatat tttggggatg cacgacagtg cctggacagg tgagtatctg ctcaatgagc
      601 aaccaattca caaactgagc aaaaaagagc gcttcgaaat ccagaagaaa acctttgggt
      661 ttgtgtttca aagctatcac ctgctcgaca atttgacggt gtatgaaaac ctcgaaattc
      721 cgctttccta ccgcaacatc aagcgcaagg aatgcgaaag catggtctgt gacgtactgg
      781 accggtttca aattgtcggc aaaaaagacc tctaccccaa tcagctctct ggtggtcagc
      841 aacaactcgt ggcgattgcc cgagccatta ttgccagtcc gaaagttatc ctggcggacg
      901 agccaacggg aaatctccac tccaaacaag ccgaagaaat catggaattg ttcaagcggc
      961 tcaacgatga aggcaccacc atcattcagg tcacgcattc ggaacgcaat gcgtcgtttg
     1021 gcaaccgggt gatcaatatt ttcgatggct ggattgcggc ggaataatga aaagcggtgt
     1081 caagccaccg cactccacag ccgtcaagcc ggcagaccac aaagc
//
LOCUS       DAOQDO010002425         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172582_length_1124_cov_2.897100, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002425 DAOQDO010000000
VERSION     DAOQDO010002425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172582_length_1124_cov_2.897100"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKZ53_27725"
     CDS             complement(<1..513)
                     /locus_tag="PKZ53_27725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ44302.1"
                     /translation="MTRELPFAHTSTTFEALPDPLRWKSLTVLSVVMFALSLDGFLTY
                     VAIPSMQEDLAARSAQMQLIVASFQIATASFLITGGRIGDLFGRKRMFILGTLGYAVC
                     WGLCCLLHNIYLIIGLFVIQGLAGALISPQVLSILSVSFNLKERNKAFGFYNAALGSG
                     ALLGLFIGGAI"
     gene            complement(773..>1124)
                     /locus_tag="PKZ53_27730"
     CDS             complement(773..>1124)
                     /locus_tag="PKZ53_27730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44303.1"
                     /translation="LDSNDVLYDRPQVIGPDGVSRPVGRNVGVSPSFSNVDLRLTRNF
                     SIKERYAVSVIAEVFNLFNRVNIDQFNRIFQRDADGNFNLPPQESGRYIVTRNRYLSS
                     FSPRLFQFGVRLTF"
BASE COUNT          338 a          264 c          275 g          247 t
ORIGIN      
        1 aattgcaccg ccgatgaaca gccccaggag ggcgcccgaa cccagtgctg cattgtaaaa
       61 tccaaatgct ttattgcgtt ctttgaggtt aaaactcact gacagaatcg aaagaacctg
      121 cggcgaaatg agtgcccctg ccagaccttg aatgacaaat aagccgatga tcaaataaat
      181 attgtgaagc aggcaacaca atccccagca aaccgcatac ccaagcgttc caaggatgaa
      241 catccgcttc cgtccaaaga gatccccaat ccgcccgccg gtaatcaaaa aactggcggt
      301 ggcgatttga aaagacgcca caatcagttg catttgggca gacctggctg ccagatcttc
      361 ttgcatggat ggaatggcaa catacgtcag gaatccatcc aatgacagag caaacataac
      421 cactgagaga acggtcaggc ttttccagcg aagtgggtcc ggaagcgctt caaatgtagt
      481 gctggtgtgg gcaaagggaa gttcacgggt catttgggtt tcaaaaggtg gggctgaact
      541 gaagtgtgac ggtacagacg tttatctcac ggctgagccg ttgagttgtt cgatcttact
      601 ccagcaaagc aaaagtctga attccagaaa aatattgggc accgcatggt gcccattgtt
      661 ttcgtggaga ttgggttgaa tattcaggta tcaattcatc tgaaatcaga agtttgaccg
      721 ttggtgagtt gagaaaacca gacgtattca cgactcagat cacaccaaac aattaaaagg
      781 taagtcgcac gccaaactgg aagagtcgtg gcgaaaacga actcaaatac cgattccggg
      841 tgacaatgta acgaccactt tcctgaggcg gaagattaaa atttccatct gcatcgcgct
      901 ggaaaattcg attgaattga tcgatgttca cccggttgaa aagattaaac acctcagcga
      961 tgaccgagac ggcatagcgc tctttgattg agaaattccg agtcagccgc aggtctacat
     1021 tcgagaaact tggcgacaca ccaacattgc ggccaactgg tcgggaaact ccatctggac
     1081 caatgacttg tggacggtca tacaacacgt catttgaatc cagg
//
LOCUS       DAOQDO010002426         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172586_length_1124_cov_2.807297, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002426 DAOQDO010000000
VERSION     DAOQDO010002426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172586_length_1124_cov_2.807297"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..>1124
                     /locus_tag="PKZ53_27735"
     CDS             318..>1124
                     /locus_tag="PKZ53_27735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44304.1"
                     /translation="MSTCDAPSKYNHCFTGTPRLITLFFGSGGLALIFLTGLVSGEWV
                     WATQADVRMVTSRLVCTPTVVQSPPPLSSSTQISAKSDLQTVEKEVQPEKRYEGFRAF
                     LQKYPQAEQTQYAREQLNATRVTLIFEYREAGKLDDAFRMGTELLKEQAPFKKNEDEL
                     LVCITLGEEAGRLSLKGNFQHLGAGELFARQAIALIDAGQKIQTTTEWDASKNRFLSQ
                     MYQTIGVANFKDHKFDDALLSLSRSIEFNCRDPFSYYLASLVYQEQYFEAA"
BASE COUNT          275 a          288 c          267 g          294 t
ORIGIN      
        1 aattcgctgt aggtaaacat tttctcagta tacactatcc ccgataatgt ctattgtggg
       61 taatgtttcc tgaaaaaagg ggccgccagg atctttccca agatccaagc gcccctttcg
      121 gtatttcacc gcttgctcaa ttcagaattg ctcccgtata gttcctggca tccacccctc
      181 ttctcaaaag ggcgtgacac tggccgccgc acaactcctc aattccaaat ccggaaatca
      241 gatcatccgc gcttcggatt caaaaaaaca cctggcttga ttgcccgatg gcctctttcc
      301 cgtgaggttg actcgatatg agcacttgtg acgctccctc caaatacaac cactgtttta
      361 ccggtacccc cagattgatc acccttttct ttgggtctgg tgggctggct ttgattttcc
      421 tgaccggttt ggtttcaggc gagtgggttt gggcaaccca ggcggatgtt cggatggtga
      481 catctcgatt ggtgtgtacc cccacggttg tgcagtcacc tcccccactt tcatcctcca
      541 ctcagatttc agcaaaatcc gatctccaga cggtggaaaa agaggttcag cctgaaaagc
      601 gatatgaggg atttcgtgcc tttttgcaaa aatatccgca agctgaacag acgcaatatg
      661 cccgtgagca actcaacgca accagggtga cgttgatttt tgagtatcgg gaagctggaa
      721 aactggacga tgcctttcgg atgggaacag aattattaaa agaacaggcc ccattcaaaa
      781 aaaacgaaga cgagttactg gtctgtatca cgcttggtga agaagcgggc cgcctgtcgc
      841 tcaaggggaa tttccagcac ctgggcgctg gtgaactttt tgcccggcag gccatcgcat
      901 tgattgatgc cggccaaaaa atccagacaa ccacggagtg ggatgcttcg aaaaatcggt
      961 ttctcagtca gatgtaccag acgattgggg ttgcaaattt taaggatcat aaatttgatg
     1021 atgccctgct ctcgttgtcc cgctcaattg aattcaactg ccgggacccg tttagttatt
     1081 atctggccag tctggtctat caggaacaat actttgaggc cgct
//
LOCUS       DAOQDO010002427         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172710_length_1124_cov_1.971001, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002427 DAOQDO010000000
VERSION     DAOQDO010002427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172710_length_1124_cov_1.971001"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..439
                     /locus_tag="PKZ53_27740"
     CDS             32..439
                     /locus_tag="PKZ53_27740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44305.1"
                     /translation="MTFFKLADPGFWEQIRQAHGSAGGAYQLVALENEKPVVVQRFLG
                     ADLEGVLYIGKSDSYLDRVIQLKKSISPQYAGKSHTCGRRYKSNPAIALKYPYELLHL
                     QFFPSGQPELLEKQMLGQYFQRFGEVPPLNAVH"
     gene            461..1063
                     /locus_tag="PKZ53_27745"
     CDS             461..1063
                     /locus_tag="PKZ53_27745"
                     /inference="COORDINATES: protein motif:HMM:NF025791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-4 family immunity protein"
                     /protein_id="HNJ44306.1"
                     /translation="MTPSEFKEKWTKGEFLTVPLPRERTKELGLAAPTVEFLAESGLP
                     EDAAPMLSFIADTPAAAEETIPLREQFNFLGPAFDPYVLIGSDGDGNPIAINTAHQDR
                     VDWLDHEDQFRAHYMNASVESLSACLLAYRTFVETIIAENGEDAYLDAHFSDAHFNTL
                     KSQIQQADSLALQQGFWKDELETLHANRLVYRPKPWWKFW"
BASE COUNT          296 a          324 c          249 g          255 t
ORIGIN      
        1 aaaccagaac aatcaaattt tatcgctgaa aatgaccttt tttaaactgg cagatcccgg
       61 tttttgggaa caaatcaggc aagcacatgg gtctgccggt ggagcatacc aattggtggc
      121 gcttgaaaat gaaaagccgg tcgtcgttca gcggtttctc ggcgccgacc ttgaaggggt
      181 gttgtatatt ggaaaatccg actcttattt ggaccgggtg attcaactaa aaaaatcaat
      241 ttcgcctcaa tatgccggaa aatcacatac ctgtggacgg cgctataaaa gtaatcctgc
      301 gatcgccctc aaatatccat atgagttgtt acatcttcag ttttttccaa gtggtcagcc
      361 agaattactg gaaaaacaga tgttaggcca atattttcaa cgatttggag aagtcccgcc
      421 cttaaatgcg gtgcactgaa cctttttaac agtcaccacg atgacaccct ctgaatttaa
      481 agaaaaatgg acgaaaggcg agtttctcac cgtgcccttg ccccgcgagc gcacgaagga
      541 actgggtctg gctgcgccca ccgttgagtt tctggccgaa tcgggtttgc cggaagacgc
      601 ggccccgatg ctgagtttta tcgccgacac gcccgccgcc gctgaggaaa ctattccgct
      661 ccgcgagcaa ttcaatttcc tggggcccgc ctttgatccc tatgtgctga tcggctccga
      721 cggcgacggc aaccccatcg ccatcaatac tgcccaccaa gaccgggtag actggctcga
      781 ccacgaagac cagtttcgcg cccactacat gaacgcctcc gtcgaatccc tgtcggcttg
      841 cctgcttgcc taccgcacgt ttgttgaaac aattatagcc gaaaacggcg aggacgccta
      901 cctggacgcc catttttcag acgcccattt caacacgcta aaaagccaaa tccagcaagc
      961 cgatagcctg gcgctccaac aaggtttctg gaaggacgaa ctggaaacac tacacgccaa
     1021 ccgcctggtc taccgcccca aaccctggtg gaaattctgg taaaaaaata ccaaccctct
     1081 aatcttccaa ccctccaacc attcaactct ccaaccttcc aact
//
LOCUS       DAOQDO010002428         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172723_length_1124_cov_1.926099, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002428 DAOQDO010000000
VERSION     DAOQDO010002428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172723_length_1124_cov_1.926099"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1124
                     /locus_tag="PKZ53_27750"
     CDS             <1..>1124
                     /locus_tag="PKZ53_27750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ44307.1"
                     /translation="PPQPQEVHDVGKGIRTFAQFDYRATASDTFRLALINSGTNFEVP
                     NTIEEQANGRDFFQRSREQTALLSWEHVVSPTGLLVTSLSERLVGVRLLPTTDPVSIQ
                     AGGLRNNVTVGIRSDYTQVIGTRHTLKTGFELTGWRLREDFSFDPRENEFEIPAFGFR
                     GRQSGGQIGGYIQDRFQPIRNLVANIGVRFDVYNLLSHERQVSPRINLAYTFSSTGTV
                     IHGAYNRFFAPPPIENLLLSANLAEDNLPPRPVKSNFYEAGVSQSFKNRLLVRLTSYY
                     RNDVNSFETSEFANVRQFLPTAFVRGKAYGVELSLRVPEIPRLHISGYFNYTAQRAFQ
                     SEKVAGGFSDEAEGEDILAAPAFDQIHTATAGFIYQEPRT"
BASE COUNT          273 a          281 c          285 g          285 t
ORIGIN      
        1 cccgccgcag ccacaggaag tacatgatgt tggaaagggt attcgcacct ttgcccagtt
       61 tgattatcgg gctaccgcat ctgacacgtt ccgactggcg ctcatcaata gtggaaccaa
      121 ttttgaggtc ccaaacacaa ttgaagagca ggcgaacgga cgtgattttt tccagcgttc
      181 gcgagagcaa acggctcttc tcagttggga gcacgtggtt tccccaactg ggctgctggt
      241 cacatcgtta tcggagcggc tggtaggggt acggttattg ccgaccacag atccggtttc
      301 aattcaagct ggtgggttga gaaacaacgt tacggtggga atccgaagtg attacaccca
      361 ggtgattggc acccggcata ccctcaaaac tggatttgag ttaacgggat ggcggttacg
      421 cgaggatttc tcttttgacc cacgtgagaa tgaatttgaa atcccggcct ttggctttcg
      481 agggcgtcaa tcaggcgggc aaattggagg gtatatccag gaccgttttc agccgatccg
      541 aaatctggtt gccaatatcg gtgtgcgctt tgatgtttac aatcttttaa gtcacgaacg
      601 gcaggttagc ccacgcatca atctggctta taccttctcc tcgactggaa cggtgattca
      661 tggggcctac aatcgttttt ttgcgccacc gccaattgaa aacctgttac tttcagccaa
      721 tctggccgaa gacaatcttc ccccgcgtcc agtaaaaagt aatttttatg aggccggggt
      781 cagccaatcg ttcaaaaatc ggttgctggt tcgactcacc agctattacc gtaatgacgt
      841 taactccttt gaaacaagtg aatttgccaa tgttcggcag tttcttccaa cggcttttgt
      901 ccggggaaag gcatatggag ttgaactctc actgcgagtc ccggaaattc cacgtttgca
      961 catttcaggc tatttcaact ataccgcgca acgcgccttc caaagcgaaa aggttgccgg
     1021 agggttttca gatgaagctg aaggagaaga catcctggcg gcacctgcct ttgaccaaat
     1081 ccatacagcc acagccggat tcatctatca ggagccacga actg
//
LOCUS       DAOQDO010002429         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172896_length_1123_cov_2.900749, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002429 DAOQDO010000000
VERSION     DAOQDO010002429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172896_length_1123_cov_2.900749"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..>1123)
                     /gene="trpD"
                     /locus_tag="PKZ53_27755"
     CDS             complement(99..>1123)
                     /gene="trpD"
                     /locus_tag="PKZ53_27755"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNJ44308.1"
                     /translation="LALGFQPQALSPGFSAPTLMITSDISDLIHLRLSEPDARELLSS
                     LTPDQVDTPLFAEIVKVLRATNECPLTVAEPFLDCCGTGGSGIPHFNVSTTTAFVLAA
                     GGVKVVKFGNRAITSQSGSFDMLEQLGIPAVVSFHHTRAIFESTGLAFLFAPQCYPAL
                     RAFTVLRKTIGVRTIFNFVGPLLHPLHPAYRLMGVSHPKMQSHVARVLSQDQTNHRSL
                     IVCGEEKLDELTPFGSNRCFDVFQGVAQEMKLSPVNTLRSSHPELRYTVGENLLLFQK
                     MIAGKLATTPEHQFVCFNAGAGFFTAGVTSSIEEGTHLARELLATGRVKTLVTQVTTA
                     YAHHAQ"
BASE COUNT          281 a          283 c          317 g          242 t
ORIGIN      
        1 caaaggcgaa actgcctggt tccagatcgg ttgagagttc gtcgggaaga aacccgaccg
       61 tgcgcgtcgc cagttcagcc cgtttgtggg ccacaatgtc attgagcgtg atgggcataa
      121 gcagttgtta cctgagtcac caaggttttg acccgacccg tggcgagcag ttcacgagcc
      181 agatgggtgc cttcttcaat tgatgaggtc acgccagcgg taaaaaagcc ggcaccggca
      241 ttaaaacaaa caaactggtg ttcgggcgtg gtggccagtt tgcctgcaat catcttttga
      301 aaaaggagga ggttttcacc cacagtatac cgcagttcag ggtgactgga acgaagtgta
      361 ttcactggtg atagtttcat ttcctgagcg acgccttgaa acacatcaaa gcagcgattg
      421 ctcccaaacg gggtcaattc atcgagtttt tcttctccgc agacaatcaa ggagcggtgg
      481 ttcgtctggt cctgactgag tacgcgtgcc acgtgcgatt gcatttttgg gtgtgaaacg
      541 cccatcaagc ggtaggctgg atgtaaagga tggagcaacg gcccgacaaa gttaaaaatg
      601 gttcggacgc caatcgtttt tcgaagcaca gtaaacgccc tcagtgccgg gtaacattgc
      661 ggcgcaaaca gaaaggccag cccggttgat tcaaaaatgg cccgtgtgtg gtgaaacgaa
      721 accacggctg gaattcccaa ctgttccagc atatcaaaac tcccgctctg actggtgatc
      781 gcccgatttc cgaatttcac caccttcacg ccgccagccg ccagaacaaa agccgtcgtg
      841 gtcgagacgt tgaagtgcgg aatgccgctt ccgcccgttc cacagcaatc cagaaatggt
      901 tctgcaacgg tgagtgggca ttcgttggtt gcacgcagca ccttcacaat ctccgcaaac
      961 aggggggtgt ctacctggtc cggcgtcaaa ctggatagca gttcgcgggc atcaggctcg
     1021 ctcaaccgaa gatgaattaa gtcggaaatg tcggaagtaa tcataagggt tggggctgaa
     1081 aacccagggc taagggcttg gggctgaaaa cccagggcta agg
//
LOCUS       DAOQDO010002430         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172921_length_1123_cov_2.675094, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002430 DAOQDO010000000
VERSION     DAOQDO010002430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172921_length_1123_cov_2.675094"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..408
                     /locus_tag="PKZ53_27760"
     CDS             193..408
                     /locus_tag="PKZ53_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNJ44309.1"
                     /translation="MHNEFTAIIEQTEGWFIAYCPEIPGANGQGRTIEECRENLVQAI
                     ALILEDRREEGLRGVPVEAIQEIVVVA"
     gene            405..593
                     /locus_tag="PKZ53_27765"
     CDS             405..593
                     /locus_tag="PKZ53_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNJ44310.1"
                     /translation="MKTGELLRHLRRHGCYLKREGAAHSLWCNPQTGQVEAVPRHTEI
                     PNQLARKICRGLSIPEIG"
     gene            complement(731..>1123)
                     /locus_tag="PKZ53_27770"
     CDS             complement(731..>1123)
                     /locus_tag="PKZ53_27770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44311.1"
                     /translation="KSFLTRLVSHLIKNDAEQRLTNSWSASIRNPIVQIQEWNVMGNH
                     TGHYVHADDWRELLENVWDTALDEAALEVNGGRHSPLQLAEMIDQEAIFQRAQHLLSL
                     TFTESTRDLPRRLRDEMKNLPGGGEAGN"
BASE COUNT          266 a          257 c          319 g          281 t
ORIGIN      
        1 cggcgtttcc tcccggccct gaaggaccgg gtctccacgc cgaagacagg atgaagccca
       61 actttggtct cttgccagcg aaagtacttt gaaacgcgtt tgggttaacc ccgaggatga
      121 ggcgtatggc gagttactta aagcctgaac tgtaacaaga tgaattggtt ccaattatga
      181 ggagaacact ttatgcacaa tgaatttaca gccattatcg aacagaccga aggatggttt
      241 attgcttatt gtcccgagat tcccggcgcc aacggccagg ggcgcaccat tgaagaatgc
      301 cgtgagaatc tggtccaggc gattgctttg attttagaag atcggcggga ggaaggattg
      361 cgaggtgtcc cagtagaggc cattcaagaa attgtggtgg tggcatgaaa acgggtgagt
      421 tgttgcgcca tttgagacgt cacgggtgct atttgaagcg ggaaggtgct gctcattcgt
      481 tgtggtgcaa tccgcagact ggccaggtgg aagcagttcc tcgtcacact gaaattccca
      541 atcagttggc ccgaaagatt tgccgaggtt tatccattcc tgaaattgga taaggtggtt
      601 tcgtccggaa gaagaaattg gagtgtgtca caagtcggta tcccccaaat gcctcctgga
      661 ctctggcctc gaaggggaag gtcagatact cagatttgtg aaagtcagct tgaggtccag
      721 gtgctctcaa tcaatttccc gcctccccac caccagggag atttttcatt tcgtcacgca
      781 gacggcgggg caggtcacgg gttgattcgg taaaggtgag tgacagcaaa tgttgggcac
      841 gttgaaaaat ggcttcctga tcaatcattt ccgcgagttg gagcggactg tgtctgccgc
      901 cgttgacctc aagggcggct tcatctaacg ctgtgtccca aacattttcc agaagttctc
      961 tccagtcatc ggcatgaaca tagtgcccgg tgtggttccc catcacattc cactcctgaa
     1021 tttgcacaat tgggtttcgg attgaggctg accaggaatt ggtgaggcgc tgttcggcat
     1081 cgtttttgat gaggtgtgag accagacggg tcaaaaacga ctt
//
LOCUS       DAOQDO010002431         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_172993_length_1123_cov_2.114232, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002431 DAOQDO010000000
VERSION     DAOQDO010002431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172993_length_1123_cov_2.114232"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKZ53_27775"
     CDS             complement(<1..730)
                     /locus_tag="PKZ53_27775"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNJ44312.1"
                     /translation="MTVHSITNSPQISQNSQYIVFQSSSNDLIPDDYNNNSDVFVAEI
                     FPPVNATLTGGGTVCTTQSTTLTATVIGGTPPYTLTLTNGGGTQTGNSPLTFTVSPLT
                     TTTYAIQSGTDANGNPITGNGNATVTVNFCAELSSLLVADTMNNRIQRFNGTTWSVVG
                     PGTVGAGTGQFRTPEAVTFNQTGRIYVADTGNNRLQWSTNGGTTWAVFATGGSGLNQV
                     NGPRGLALDQNGNLYVADSGNNRVV"
BASE COUNT          265 a          263 c          289 g          306 t
ORIGIN      
        1 gaacaacccg gttattgcca ctatcagcca catagagatt gccgttctga tcaagtgcca
       61 atccccgtgg tccattcacc tgattcagcc cactgccacc tgttgcaaag acggcccagg
      121 ttgtaccgcc attggttgac cattgaaggc gattgtttcc tgtatcagcc acataaattc
      181 gcccggtttg gttaaaggta acggcttctg gagtgcggaa ttgacctgtg ccggcgccta
      241 ctgttcccgg gccaaccact gaccaggtgg ttccattaaa ccgctgaata cggttgttca
      301 ttgtgtcggc aaccagaagc gacgaaagct ccgcgcagaa attgacggtc acggttgcat
      361 ttccatttcc agtgatggga ttgccgttgg catctgtccc agactgaatg gcataggtgg
      421 tggtggttaa tggagacacc gtaaaggtca gtgggctgtt tccagtttgg gttccgccgc
      481 cgttggtcaa ggttagcgta tagggcggtg ttcccccaat tactgtggcg gtgagggtgg
      541 ttgattgagt cgtacaaacg gttcctccgc cagtgagtgt ggcattcacg ggtggaaaaa
      601 tctcggccac gaaaacatca gaattgttat tgtaatcgtc aggtatcaaa tcattcgaag
      661 aactttgaaa cacgatatat tggctatttt gactgatttg gggtgaattg gtgatactgt
      721 ggaccgtcat tgagggtgat ccattcactc cactgacgag ccgaatttgg ctgttgcccc
      781 ggtcaaaaac aaatatatca gttgtcctac tcgtatcagt ttggcctgga accaaattgg
      841 tagccatgct ctcaaatgca atgaattgcc cggtgctact gatggtggga gaaactgaac
      901 tttgattccc agttgtggca ttggttccag cagctcggct taccaactcg gtcgttccag
      961 agatccgatt atatacaaac acgtcccacg tctggtttgc atcgttctga ttggatacaa
     1021 gatcagtcgc gacactttca taggtaatat aatttccatc tccactgatt gctggatgaa
     1081 aagaagagta attccctgta gttgtagccg ttccgacagc atg
//
LOCUS       DAOQDO010002432         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_173545_length_1121_cov_2.741088, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002432 DAOQDO010000000
VERSION     DAOQDO010002432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173545_length_1121_cov_2.741088"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKZ53_27780"
     CDS             complement(<1..214)
                     /locus_tag="PKZ53_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNJ44313.1"
                     /translation="MLTIWDVIKSPVITEKAMDMKEHTTETGQLLTFKVDPRANKIQI
                     KNAVEKIFNVQVAAVRTANFQGKEVRR"
     gene            complement(293..916)
                     /gene="rplD"
                     /locus_tag="PKZ53_27785"
     CDS             complement(293..916)
                     /gene="rplD"
                     /locus_tag="PKZ53_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNJ44314.1"
                     /translation="MPVVKVRNLKNEEVGEIELSERVFGAPLNKALIYEAVKEFRARG
                     RAGTVATKTRGDVSGAGRKLWKQKGTGRARIASLRSPLWKGGGNVHGPQPRDWSYQIP
                     KKMRRGAIRAALSERLREGGVIVVDDFSLQQHKTRDLVAILSKLGVDKRALLVDSLEN
                     RNLILASRNLPDVDHTNSFGLNIFNVLLHEQIVLSKRAVGEIEKILA"
BASE COUNT          245 a          316 c          253 g          307 t
ORIGIN      
        1 cacggcggac ttcttttccc tggaagttcg cggttcgaac ggcggccacc tggacgttga
       61 aaatcttttc aacggcattc ttgatctgaa ttttattggc gcgcggatca accttgaagg
      121 tcagcaactg ccccgtttcg gtggtgtgct ccttcatatc catggctttt tcagtgatca
      181 ccggcgattt gattacatcc caaattgtca acataggtcc ctctcctgca gttttcagtt
      241 ttaatcgtga tccgtatgaa gtctgtcaac tggtccgctt aaaactcaaa acttaggcca
      301 aaatcttctc gatttcccca accgcgcgct tgctcaacac aatctgctcg tgcaggagaa
      361 cattgaaaat attgagtcca aagctgttgg tgtggtcaac atctggaaga ttgcgcgagg
      421 ccagaatcaa attccggttt tcaagtgaat ccaccagtaa tgcccgcttg tcaacgccga
      481 gtttggacaa gatcgcgacc agatcccgag tcttgtgctg ttgaaggcta aaatcatcaa
      541 ccacaatcac cccgccttca cgcaggcgtt ctgacaacgc ggcgcgaatc gcgccacgcc
      601 gcattttctt cgggatttga tagctccaat cgcggggctg agggccgtgt acgttaccgc
      661 cccctttcca caacggggaa cgaagactgg cgatccgcgc ccgaccagtt cctttttgct
      721 tccacagttt tcgtccggcg cccgaaacat caccgcgggt ctttgttgcc acagttccag
      781 cccggcctcg cgcccgaaat tctttgacgg cttcataaat caacgctttg ttcagcggtg
      841 cgccaaagac tcgttcgctt agttcgattt caccgacttc ttcatttttt aagtttctga
      901 cttttacaac cggcatatca gccctctcac catttgcgat gatagtttca gcacctgaac
      961 cgtgttttgg ttcgtgggaa gtgtcccagg ttcagatggg atggcgctac gcaccagttt
     1021 tttgaatcag cacatacccg ccatttggac caggaatgct gccctttacg atcagcagat
     1081 tcttttcaga atcaacccgc acgatcttca agttcttgat g
//
LOCUS       DAOQDO010002433         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_173607_length_1121_cov_2.239212, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002433 DAOQDO010000000
VERSION     DAOQDO010002433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173607_length_1121_cov_2.239212"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKZ53_27790"
     CDS             complement(<1..778)
                     /locus_tag="PKZ53_27790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ44315.1"
                     /translation="MQKKSAIATTITTTSSNKEFDFKQEAIKQWTHDPCGLLGAKGFE
                     IGTGEFYERVDHNRYVEYAPWMKSVMPFADFQGRKVLEIGFGMGTDLFQFARGGAEVY
                     GVDLSPEHLRIASERFAFFGLPANLQLADAENLPFPDASFDAVYSFGVIHHIPNIVQA
                     VREIYRVLRPGGQAIISVYHKNSAFYYFCVLGGYLVHFRFLNESFQANLSRIEYREHS
                     DACPLVKVLSRNELRNLFSQFPEVGIECHHLAATHFGSLQR"
     gene            940..>1121
                     /locus_tag="PKZ53_27795"
     CDS             940..>1121
                     /locus_tag="PKZ53_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNJ44316.1"
                     /translation="MVAVLWRNLKITCEMIKIEHTLFALPFAFLGAFLAAHGVPSLNK
                     IFWITVAMVGARSAAMA"
BASE COUNT          282 a          269 c          276 g          294 t
ORIGIN      
        1 gccgttgcaa tgacccaaaa tgagtggcgg ccaggtgatg gcattcaatt ccgacttcgg
       61 gaaattgact gaataaattc cgtaactcat ttctggagag aactttaacc agtgggcagg
      121 cgtcgctgtg ttcgcggtat tcgatgcgtg acaggttggc ctgaaacgat tcgttcagaa
      181 atcgaaaatg caccagatag ccgcctaaga cacagaaata ataaaaggct gagtttttgt
      241 gatagaccga aatgatggcc tgccctccgg gccgcagtac ccggtaaatt tcacgaactg
      301 cctgcacaat gttgggaata tggtggatga caccaaatga gtacaccgcg tcaaacgaag
      361 catccggaaa cggaagattt tcggcatccg ccagttgtaa attggctgga agtccaaaaa
      421 acgcaaaacg ttcgcttgca atccggagat gctctgggga aagatccacg ccatacacct
      481 cggcgccacc cctcgcaaat tgaaacaggt ccgttcccat cccaaatccg atttcaagga
      541 ccttccggcc ctggaagtca gcaaacggca tcactgattt catccacggg gcatattcaa
      601 catagcgatt gtggtcaacc cgttcataaa attcaccggt cccaatctca aagcctttgg
      661 ccccaagcag cccgcatggg tcgtgagtcc attgcttgat ggcttcctgt ttaaagtcaa
      721 actctttgtt ggaactggtg gttgtgatgg tggttgctat tgccgatttc ttctgcattt
      781 tgaaagaata gattttccga tcaatttttt agtgcttggt gcttggtgtt tagtgcctgg
      841 tgttttagtg cttaggaatc agtaattggt gctttggaac caataccttc caagaaccaa
      901 gaaccaagaa ccaagaaccc tgcttcccga ggatttttta tggttgctgt gctctggcgt
      961 aatctgaaga ttacctgcga gatgatcaaa atcgaacata ccctgtttgc gttgccgttt
     1021 gcctttttgg gggcatttct ggcagcacac ggcgttcctt cactcaacaa gattttctgg
     1081 attaccgtcg ccatggttgg ggcccgaagt gcggccatgg c
//
LOCUS       DAOQDO010002434         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_173884_length_1120_cov_2.453521, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002434 DAOQDO010000000
VERSION     DAOQDO010002434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173884_length_1120_cov_2.453521"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKZ53_27800"
     CDS             <1..496
                     /locus_tag="PKZ53_27800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44317.1"
                     /translation="HSIAQETFDKVLSYRGITQMSHNYVQILTALEAAGLTHDLTPLD
                     KHSIQKMKEENPGIPGDYLEYLQDIGWGKIGESYMVYSGPIDPEDIFGVREDANLEHI
                     TLFGDGFSGDCDGFSIEKNWELVYIDHVSLKVYAWNLTFEAFIKKKINRILQNIKKTK
                     DLSR"
     gene            complement(498..1019)
                     /locus_tag="PKZ53_27805"
     CDS             complement(498..1019)
                     /locus_tag="PKZ53_27805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44318.1"
                     /translation="MKYTLSPGISLLAAVLAFLCIIPEPVRAAQKYAPVVGRWQAKVQ
                     FEDTTLEFDFETLGEGVYGYGNGSIFLLEPQTRLVATYPAAWDNHSVETIHISGEISL
                     PAVAGKKRTPGTLILRIHYEPKKGYVGTALFIGEDLTQRKGTIEMKRTKGPEELLKSS
                     SLVTPDHLTGITG"
BASE COUNT          302 a          250 c          249 g          319 t
ORIGIN      
        1 acattcaatc gctcaggaaa cttttgacaa ggttcttagc tatcgaggaa taacacaaat
       61 gtcacacaat tatgtgcaga tattgaccgc tctggaggcg gctggactta cgcacgatct
      121 cactcccctc gataaacatt ccattcaaaa aatgaaggaa gaaaatcccg gaatacccgg
      181 tgattacctg gaatatcttc aggatattgg ttggggcaaa attggtgaaa gttatatggt
      241 gtactctggg cccattgatc cagaagacat ttttggagtt cgggaagatg ccaacctgga
      301 acatatcacc ctttttggtg atgggttctc tggagattgt gatgggttct caattgagaa
      361 gaattgggag cttgtctata ttgatcacgt gagccttaaa gtctacgcgt ggaacctgac
      421 ctttgaagcg tttataaaga aaaaaatcaa tcggatactc caaaacatta agaaaacaaa
      481 agatttatca cgttgaccta cccggtaatg ccagtaaggt ggtctggtgt aaccagagat
      541 gatgatttga gcaactcctc aggtcctttt gtccgcttca tttcaattgt gcctttgcgc
      601 tgggtcaaat cctcgccaat aaataacgcc gtgccaacat agcctttctt cggttcataa
      661 tggattctca aaatcagcgt tccaggtgtc cgctttttcc cagccactgc tggcaaactg
      721 atttccccgc tgatatggat ggtttcaaca ctatggttgt cccacgctgc cgggtacgtt
      781 gcgaccagtc tggtttgagg ctccaaaaga aagatcgagc catttccata tccgtacacc
      841 ccttccccaa gcgtctcaaa atcaaattcc aaagttgtat cttcaaattg aactttggcc
      901 tgccaccggc caacgacagg tgcgtatttc tgagccgccc gaactggctc ggggatgata
      961 cagagaaaag ccagaaccgc agccagaagg ctgattccag gtgatagagt gtatttcatg
     1021 ttcttcgctc ctgctttgaa ggttattggg tttctatgtc ttgaaatttc ataaactatt
     1081 gaagaattgg gatttatttt ctgtgtattc cgtgtattcc
//
LOCUS       DAOQDO010002435         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_174329_length_1119_cov_1.653195, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002435 DAOQDO010000000
VERSION     DAOQDO010002435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174329_length_1119_cov_1.653195"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1004
                     /locus_tag="PKZ53_27810"
     CDS             111..1004
                     /locus_tag="PKZ53_27810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44319.1"
                     /translation="MSASVCTPQKWQRSLFVTGAVSMILLLPALFPFAQESQPGSRSI
                     LRVYSEFEANRPEKKIPAKRPSLPRPPKITSPQVPNSVGITLWRILDSGSRKIERADT
                     TSNGQLLKAERLSALSRLKPGEKVRLTIEVPREGYLYIVSREEYADGTLGDPYLVFPS
                     KQILGGDNHVAPGQLTGIPAITDNPPFFELQRSGPAQVAELISFLILPEPIPNLVVTR
                     DPIELDQKLFTGWQRWEAPVQQLELPATRNQRYTQAEQAAESKGVSRRLRHGDPLPQN
                     LLLTDSKSESVMVTIPLKFVR"
BASE COUNT          278 a          306 c          267 g          268 t
ORIGIN      
        1 tcccgctcga ccaccagacc accctccacc caccaggttg ccgtcattgt gtaatgtcaa
       61 atgactgaac agatgacccg aatctggttt tccgccaaag gatacctgtc atgtctgctt
      121 ctgtgtgtac gcctcaaaaa tggcagcgaa gcctgttcgt caccggcgct gtctcgatga
      181 tacttttgtt gccggctctt tttccttttg ctcaggaatc acaacctgga tccaggtcaa
      241 ttttacgtgt ctattcagaa tttgaagcca accgacctga gaaaaagata ccggcgaaac
      301 gtccgtcgct cccacggcct ccaaaaatca ccagccctca agtaccgaat tcagttggga
      361 ttactctctg gcgaatcctg gacagcggaa gccgaaaaat tgaacgtgct gataccacaa
      421 gcaacggtca attactgaaa gctgagcgtc tgtcagccct ctcgcggctt aaacccggcg
      481 aaaaagtccg cctgacaatt gaagttcccc gcgaaggata cctctacatt gtcagtcggg
      541 aggaatatgc cgatggaacc ctgggtgacc cgtacctggt ttttccaagc aaacaaatcc
      601 tcggaggtga taaccatgtc gctcccggac aattgacggg gattccggcc attaccgaca
      661 acccgccttt ctttgagttg caacgcagcg gaccagctca ggtggcggag ctgattagtt
      721 ttcttatttt gccggaacca atcccaaacc tggtggtcac ccgcgaccca atcgaacttg
      781 accagaaatt gtttactggc tggcaacgct gggaagcacc ggttcagcaa ctggagctcc
      841 ccgccaccag gaaccaacgc tatacccaag ccgaacaggc tgccgaatca aagggagtgt
      901 cccgccggtt acgtcatgga gatcccttgc ctcaaaacct gttactcact gattccaaaa
      961 gcgaatcggt gatggtgacg attccactga agtttgttcg gtaagatcag tgtgtctggc
     1021 tagttgaatt cttacaaagt gttacggtct ccggcattga cgcaggaaga ccaatctagt
     1081 gtgttttgaa taacttggga ggttgagatg tttgtattt
//
LOCUS       DAOQDO010002436         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_174434_length_1118_cov_3.302916, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002436 DAOQDO010000000
VERSION     DAOQDO010002436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174434_length_1118_cov_3.302916"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKZ53_27815"
     CDS             complement(<1..1051)
                     /locus_tag="PKZ53_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNJ44320.1"
                     /translation="MRITVDSLIPFSINLQSGVYNLSKFKYCIVLILPVSACIAFLTT
                     GVSTLLPVLFFFGVVPAVELILKPDHHNNDHSERAILANEAFFNWLLYSLVLVLISVA
                     VLFVFTISAPSLTTPNLIGRILSMGMMCGVAINVGHELGHRSNRLEQLLGEIALLISL
                     ENQFLPYHNYCHHKNVATPQDPATARRNESVYRFWIRSQIGSYVQAWQVEINNQRRKH
                     RHPFSLQNRMVCYTAAQICLVLVLCFRFGLKTMLAFIAAAIIGKLVLETVNYIEHYGL
                     TRKINAAGKYERVLPVHSWNSDHLFGRALMFELPRHSDHHFKANKPYQILDSFPESPQ
                     LPTGYPGMMILALFPP"
BASE COUNT          319 a          256 c          284 g          259 t
ORIGIN      
        1 gtggtggaaa gagcgccaaa atcatcatcc ctggataccc agtcggtaac tgagggcttt
       61 ctggaaagga atccagaatt tgatagggtt tgttcgcttt aaaatgatga tcagaatgac
      121 ggggcagttc aaacattaag gctctgccaa acaggtggtc agaattccag gaatgaaccg
      181 ggaggacacg ttcatatttg ccagccgcat tgatttttct ggttaacccg tagtgctcga
      241 tatagttcac cgtttcaagc acaagttttc cgatgatggc ggcggctatg aacgccagca
      301 ttgtcttgag tccaaatctg aagcacaaca ccagcaccag gcagatttgg gctgccgtgt
      361 aacagaccat tcgattctga agcgaaaacg ggtggcggtg tttccgtcgc tggttgttga
      421 tttcgacctg ccacgcctga acgtagctcc caatctggga acgtatccaa aaccggtaaa
      481 ctgattcgtt gcgacgcgcc gttgccggat cctgcggcgt ggcaacattt ttatggtggc
      541 agtaattgtg atagggcaaa aactgattct ccaaagaaat cagcaaggca atttcaccca
      601 gcaactgttc caaccgattt gaacgatgcc cgagttcgtg acccacattg atggccacgc
      661 cgcacatcat tcccattgaa agaatcctgc caatcaaatt aggagtggtt aaactgggag
      721 ctgagattgt aaaaacaaaa aggacggcta cggagataag caccagaacc agactgtaca
      781 aaagccagtt gaagaaggct tcattggcca ggattgcccg ttccgaatgg tcattgttgt
      841 gatgatcggg cttgagaatg agttccaccg ctggaacaac cccaaagaaa aagagtaccg
      901 gcagcagcgt tgaaactcca gttgtcagga atgcgataca ggctgaaacc ggtaaaatca
      961 agacgatgca gtacttgaac ttgctcaggt tataaacacc tgactgaaga ttgattgaga
     1021 atgggatgag cgaatcaact gtgattctca tgccggaggt cccctttcca caaatttctg
     1081 ggccaaggat accgacaacc gctggaaatc agtttgac
//
LOCUS       DAOQDO010002437         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_174504_length_1118_cov_2.356538, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002437 DAOQDO010000000
VERSION     DAOQDO010002437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174504_length_1118_cov_2.356538"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKZ53_27820"
     CDS             complement(<1..653)
                     /locus_tag="PKZ53_27820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44321.1"
                     /translation="MDVSNSLAIHKLKISRVGFELRARNRAELPSFLGSTLRGAFGHA
                     LKATTCLEETRAECAQTCRQPAQCPYTYVFETQLSPHFAATKRHEDLPHPYLIVPPKL
                     NAAAPSRRYAAGDTFSFQLVLVGAATDHLPAAVLAVHEMARSGLGYQRIPFDVISFQA
                     PAASESDINTLRGATTTLGELIAPKLESLQDATSVRLRFVTPVRIRLGGDLQSKLPFS
                     "
     gene            complement(671..>1118)
                     /locus_tag="PKZ53_27825"
     CDS             complement(671..>1118)
                     /locus_tag="PKZ53_27825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44322.1"
                     /translation="LDDALRRAQEVLKPTGTLLIEDFAFAEADVDTIHWFVKKLRCET
                     IHRWINLHSGEFVTTLLEASDPIAAWHHDHSHHIHSIHTMTGAVADRFHLKSTLAVPY
                     LYRYLIPVLPPTQIAAALVEELFHAEMRAAEMGNISLIGRRMVAYF"
BASE COUNT          269 a          289 c          320 g          240 t
ORIGIN      
        1 gaaaacggga gttttgattg caggtctcct cctaatcgaa tgcgcaccgg cgtcacaaac
       61 cgaagcctca ctgaggtagc atcctggagc gattccagtt taggggcaat cagttcaccc
      121 agggtcgtcg tggccccacg cagggtattg atgtctgatt ctgaagccgc cggagcctga
      181 aacgagatca cgtcaaatgg aatgcgctga tatcccaacc cggaccgggc catttcgtga
      241 acggccagca cagccgctgg aagatggtcc gtcgccgctc cgaccagcac cagttgaaat
      301 gaaaaggtgt cacctgccgc atatctccga ctgggagcgg cggcgttgag tttgggaggg
      361 acgatcaggt atgggtgagg caggtcttcg tggcgtttgg tagccgcaaa atgcggagac
      421 aactgggttt caaacacata ggtataggga cattgggctg gctgacggca cgtttgagca
      481 cattccgctc tggtttcttc cagacaggtg gtggctttca aggcatgccc aaacgctccg
      541 cgcaacgttg aacctaaaaa ggatggaagt tcagcccggt tacgcgctcg caactcaaaa
      601 ccaacccggc tgattttcag cttatgaatc gcaagagaat tggatacatc catacaaata
      661 ccgtctaaaa ttagaaatac gccaccattc ggcggccaat cagtgaaatg tttcccattt
      721 cggcggctcg catttcagcg tgaaagagtt cctcaaccag agccgccgct atctgagtcg
      781 gcggcagaac cgggatcagg taccggtaca gataaggcac cgccagggtt gacttgaggt
      841 gaaaccggtc ggcaacggcc cctgtcatgg tgtggataga atgaatgtgg tgactgtggt
      901 cgtggtgcca ggcggcaatc gggtcactgg cttcgagcaa cgtcgtgaca aattctccgg
      961 aatgcaaatt gatccatcgg tgaatggttt cacaccgcaa tttcttgacg aaccagtgga
     1021 tcgtatccac atcggcctca gcaaaggcaa agtcttcaat cagaagcgtt cctgtgggtt
     1081 tgaggacttc ctgcgcccgg cgcagtgcgt cgtcaagt
//
LOCUS       DAOQDO010002438         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_175530_length_1115_cov_1.723585, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002438 DAOQDO010000000
VERSION     DAOQDO010002438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175530_length_1115_cov_1.723585"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKZ53_27830"
     CDS             <1..396
                     /locus_tag="PKZ53_27830"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNJ44323.1"
                     /translation="IGSNDLIQHTLAVDRANPQVAHLYEPLHPAVIRSLKQVVDAGKI
                     AGTLVQICGEMAANPTCAVVLLGLGLRNFSMSPSAIPNIKRLVRSVRVSEAERLVTRV
                     MELDTPRAIEQFLEGELSKSVPHFFANSQ"
     gene            complement(383..811)
                     /locus_tag="PKZ53_27835"
     CDS             complement(383..811)
                     /locus_tag="PKZ53_27835"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNJ44324.1"
                     /translation="MTHHSLSASVTHLELGQWGEGLATSYLKQNGYQIIARNVTLPLG
                     RTPEGTPITGELDIVALDGQTLVFVEVKTRSSDELALPEAAVTPAKQRKLARTARRYR
                     RLIGPVDVPYRFDVVSIVASGQVAPKIKLLKNFFEVATAN"
BASE COUNT          252 a          282 c          301 g          280 t
ORIGIN      
        1 atcggcagca atgatttgat tcaacatacg ctggcggttg atcgtgccaa tccgcaagtg
       61 gcccatctct atgaaccgct ccacccggca gtgattcgga gtctgaaaca ggtggttgac
      121 gctgggaaaa ttgccggaac actggtgcaa atctgtggcg aaatggccgc taacccaacc
      181 tgtgccgtgg tgttgctggg tctggggttg cgcaacttca gcatgtcgcc gtcagccatt
      241 ccaaatatca agcggctggt gcgtagcgtt cgagtttcag aagctgagcg actggtgaca
      301 cgcgtgatgg aactcgatac cccacgagcg attgaacagt ttctggaagg cgagctttca
      361 aaatcggtgc cgcacttttt tgctaattcg cagtagccac ctcaaaaaaa tttttcaaca
      421 atttgatctt cggcgccacc tgacctgacg ccacgatact caccacatca aagcgatatg
      481 gcacatcaac tggcccaatc agccggcgat agcgtctggc ggttcgggcc agtttgcgtt
      541 gtttggcggg agtgacggct gcttccggga gcgccagttc gtctgacgag cgggttttga
      601 cttcgacaaa caccagggtt tgaccatcaa gggcaacgat gtcgagttcg ccagtgatgg
      661 gcgtgccttc cggtgttcga cccaggggca gggtaacgtt gcgggcgata atctgatagc
      721 cattctgttt gaggtagctg gtcgccagac cttctcccca ttggccaagt tcaaggtgtg
      781 tgacagatgc agacagggaa tggtgtgtca tgggcggttc aatataaatt taatttcaac
      841 caactctgct atatcagagc aagtcgcgtg caacaggaca cagtgagcgg gcttataatt
      901 cagtaagact gcggctggaa gttaaggagc aatgggagtt gttgccctgg caacatttcg
      961 ctacaattcc gcctcgtttc tttggtcacc cttttcaatt ccaactcaat tttgaaaacc
     1021 gaaggagaat tcgccactgt gagagtccgt tcacttctca tccagcattg tctccgacct
     1081 gctttccggg cggtgtttca tccgtttttc caaat
//
LOCUS       DAOQDO010002439         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_175537_length_1115_cov_1.695283, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002439 DAOQDO010000000
VERSION     DAOQDO010002439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175537_length_1115_cov_1.695283"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1115)
                     /locus_tag="PKZ53_27840"
     CDS             complement(<1..>1115)
                     /locus_tag="PKZ53_27840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44325.1"
                     /translation="PPGQWALIRPVTRLPNDSLVTCVIKDGLPSAEGPTPLAQPFALT
                     FQTAKPFHLTWGSKKGDDWYASLGAIIGFSNHLKLESVQSEFVTVEPPVEDISVGTNS
                     TVVHIFGNFQAEQIYTITVKPGICDVYGQTLAECETIQICLNHEPSTLHFPSEYVLTL
                     DPAGRLQLPVYTTNVEQCQVQVYSVKPEDWPAYTGLWKFLEQHPDDPLPTPPGKKGVE
                     TTLRPEVRPDLMVETPLDLSPAFPNQIGHAVVVIHGPLDFHQVEGFETFYSKVYLLWV
                     QSTRLAAEILIQKPRSVAWVTSLESGQPIAGAQVTLYPGGIKALTTSTGLTRLPLIQP
                     VEDQPNWTLVQKGPDSVLLPEGNEYWHLETGAEPKLR"
BASE COUNT          265 a          277 c          297 g          276 t
ORIGIN      
        1 gcgcagtttt ggttcggcgc cggtttccag atgccagtac tcatttccct ctggcaaaag
       61 aacgctatcc gggccttttt gaaccagcgt ccagttgggc tggtcttcaa ctggttgtat
      121 cagcggcaat cgtgtcagcc ccgtggacgt ggtcagggct ttgattcctc ctggatacag
      181 cgtgacctga gccccagcaa tcggttggcc tgattccagc gacgtaaccc aggccactga
      241 tctgggtttc tgaatcagaa tttcagccgc caatctggtt gactgaaccc aaagcagata
      301 cacctttgag taaaaggttt caaagccttc aacctgatga aagtctaatg gcccatgtat
      361 caccaccacg gcatgtccaa tttgatttgg gaaggctggg ctgagatcaa gtggtgtttc
      421 aaccatcagg tctggccgca cctcaggccg aagcgtcgtc tcaacccctt tctttcccgg
      481 cggcgtgggg agtggatcat ccgggtgttg ttcgagaaac ttccagagcc cggtataggc
      541 tggccaatcc tcaggcttga cggaatagac ctggacctgg cattgctcaa cattggtggt
      601 gtagaccgga agctggagcc gtccggctgg atcaagcgtc aagacatatt cagatggaaa
      661 gtgtagcgtt gaaggctcgt gattaaggca gatttgaata gtttcacatt cggccagtgt
      721 ttgaccgtaa acatcacaaa ttcctggctt tacggtaatg gtgtatatct gctcagcttg
      781 aaaattgcca aagatgtgaa cgacagtcga attggtgcca accgagatat cttccacagg
      841 tggctcaacg gttacaaact ctgattgaac actttccagt ttcaaatgat tcgaaaaccc
      901 gataatcgcg cctaaactgg cataccagtc atctcctttt ttagatcccc aggtaaggtg
      961 aaaaggtttg gccgtttgaa aggtcaaagc aaagggctgt gccaggggcg tgggaccttc
     1021 agccgagggc aacccatctt tgataacaca ggtcacgagg ctgtcatttg gcaagcgtgt
     1081 gaccggtcga atcagagccc attgtccagg cggtg
//
LOCUS       DAOQDO010002440         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_175841_length_1114_cov_1.749764, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002440 DAOQDO010000000
VERSION     DAOQDO010002440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175841_length_1114_cov_1.749764"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..>1114
                     /locus_tag="PKZ53_27845"
     CDS             297..>1114
                     /locus_tag="PKZ53_27845"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ44326.1"
                     /translation="MVRTICFLVLFIFPVLIQARPLPPAKGKAVDTAIKAYMARHGIP
                     GLSLAVARDGRVIWSKGYGFADLENSVPATSETVYRSASIGKSITATAAMRLVEEKKL
                     DLDQPLQNYCTAFPLKPWTITPRHLLTHTSGIRHYGGPRNREEQTSTVHYDSVVKALA
                     PFKDDPLLFEPGTDYSYSTYGYNVLGCVLEGAAKMPFMEVIKNYVFDPAGMTHSRDDD
                     PAVIIPHRAAGYARVDGRLQVAIHVDMSNRLPAGGYVTTAPDLAAFAIKFMDCKL"
BASE COUNT          268 a          287 c          293 g          266 t
ORIGIN      
        1 ggtgtggaac cagggaaccg aattgacgag ttcgaggctc tgggcctggg gaaataccga
       61 acgatggaga actggattta aatttctaag attggctcaa cttgccccct gaacgattca
      121 tccctatcat ctagccacac acaccaccgg gccaaaaaat caattgagaa ttagattctg
      181 ccttctgatg gcaagaacac tcacggttga gagtgaccac ctgtgcgttt gttgtggcac
      241 accagtcgaa agcggtttcc cagggccatc caggcaggat tcaggagtaa aaaccaatgg
      301 tccggactat ctgttttctg gttctgttca tttttccggt attgattcag gcacgtcccc
      361 tcccaccagc caaaggtaaa gccgttgata ccgcgatcaa ggcctatatg gcccggcatg
      421 gtattccggg attgtcgctt gcggtggccc gtgatggacg ggtgatctgg tcaaaaggat
      481 atggctttgc ggatcttgaa aattccgttc cggcaacctc agagaccgtc tatcggagcg
      541 cttcaattgg aaaatcaatc acggcaacgg cggcgatgcg gctggtcgaa gaaaaaaagc
      601 tcgacctgga tcagcctctc cagaattact gtacggcgtt tccactaaaa ccgtggacca
      661 ttacacctcg ccatctcctg acacatacat ctgggattcg ccactacgga gggccccgta
      721 accgtgaaga gcaaaccagc acggttcatt atgacagcgt ggtcaaagca ttggctccat
      781 ttaaagacga tccgctgttg tttgaacctg gaactgacta ttcttacagc acttacggtt
      841 ataatgtatt ggggtgtgtc ctcgaaggtg cggccaaaat gccgtttatg gaagtcatca
      901 agaattatgt gtttgatcca gccggaatga cccattcgcg cgacgatgat ccggcggtga
      961 ttatcccgca tcgggccgcc gggtatgccc gggttgatgg ccggttgcaa gttgccatcc
     1021 acgtagatat gagcaaccgg cttccggctg gcggttatgt cacaaccgcg cccgacctgg
     1081 cggcctttgc catcaagttt atggattgca agct
//
LOCUS       DAOQDO010002441         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_176264_length_1112_cov_3.773888, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002441 DAOQDO010000000
VERSION     DAOQDO010002441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176264_length_1112_cov_3.773888"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="PKZ53_27850"
     CDS             <1..>1112
                     /locus_tag="PKZ53_27850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44327.1"
                     /translation="YVLDVETIKSTGIIAIDLYYQERKKNGDWGKAKQTHLKREHFLV
                     HPNPQDRQLLLTLAGMNALGSNTLSVSGSLQAYLGRESYKPLQYYVGGPDLEGVFPQL
                     CATNRFFIIQAQQGERKFIGPLAWDPHEKPWEFHLGLNRVTSKSSDFETQLLLKREEK
                     QLSPSEVLLLLSDGYLLTRTQVSKFNHFGAFPLVAYLRGEVPLVIPEAELDTFLHEMT
                     QLPSAPPLQLPASHQVQVNQMEPLPFLSIRRNQPSAILKGNLEFVYDQLRFKMDDTSG
                     GHYDAQTKHFYRRNRIAEKQAAQILTQHPGVEEHTFRDTGKGKSTSFQFPPQELPRLV
                     SKLTAQGWRVEAEGKLYRTPGQVSIQVKSGLDWFEL"
BASE COUNT          290 a          308 c          262 g          252 t
ORIGIN      
        1 gtatgtcctt gatgttgaaa ccattaaatc aacgggcatc attgccattg acctgtatta
       61 tcaagaacgg aagaaaaacg gcgactgggg caaagccaaa caaacccatt tgaaacgaga
      121 acatttcctg gtccatccga acccgcaaga ccgccagttg ctgctgaccc tggctgggat
      181 gaatgccctg ggcagcaata ctctctcagt gagcggcagt ctccaggcat atctcgggcg
      241 tgaatcctat aaaccacttc agtactacgt cggcggacct gatctggaag gtgtcttccc
      301 tcaactctgt gccaccaacc ggttttttat cattcaggca cagcaaggtg agcgaaagtt
      361 tattggcccg ctcgcctggg atccacatga aaaaccgtgg gagtttcatc tcggcctcaa
      421 tcgagtaacc agcaagtcgt cagactttga gacccagtta ctgcttaagc gggaggaaaa
      481 acaactctcc ccgtcagaag tacttttgct gttaagcgac ggctatttac ttacccgcac
      541 ccaggtgtcg aaatttaatc actttggggc ctttccgctc gtcgcctatc tgcggggaga
      601 agtaccgctg gtgattccag aagctgaact tgataccttt ctccacgaaa tgacccagct
      661 tccgtcagcc ccaccactcc agcttccagc ctcacaccag gttcaagtga accagatgga
      721 accgctgccg tttctttcaa ttcgacgcaa tcaaccttca gctatcctca aaggaaacct
      781 ggagtttgtc tatgaccagc tccggtttaa aatggatgat acgtcaggtg gacactatga
      841 cgcccagacc aaacattttt accgacggaa tcgaatagct gaaaaacagg cggcccagat
      901 cttgacgcaa catcctggtg ttgaagagca taccttccgc gacacgggta aaggcaaaag
      961 cacctcgttt caattccctc cccaggaact cccccgactg gtctcaaaac tgacagctca
     1021 ggggtggcga gtggaggcgg aaggaaagtt gtaccgaaca cccggacagg tttcaatcca
     1081 ggtcaaatcg ggccttgact ggtttgagtt gc
//
LOCUS       DAOQDO010002442         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_176280_length_1112_cov_3.156102, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002442 DAOQDO010000000
VERSION     DAOQDO010002442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176280_length_1112_cov_3.156102"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKZ53_27855"
     CDS             <1..291
                     /locus_tag="PKZ53_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44328.1"
                     /translation="QRDQLDKAVLWWKRAVTTGDQLDANCDVQTQEAIQKLIRRIGER
                     LIREQKTGSVSFRWTMFHAHLRKDSSILREAILKFPALPTIDPKEAESQTQE"
     gene            390..>1112
                     /locus_tag="PKZ53_27860"
     CDS             390..>1112
                     /locus_tag="PKZ53_27860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ44329.1"
                     /translation="MLILTLCLFTLPLRADEIDDLVKQQMEADHIPGLSIAVIHKGKI
                     VKLQGYGVSDLEKKTPVTPHTMFKIASVSKQFIASAIVLLAADGKLNLNDSMTRYFPD
                     APETWKPITLKHLLSHTSGLVRESPAFHPIFAKSDLEIVKATYPVALKFQPGERWEYC
                     NVGYFALADIIRQVSGKPWPEFCQERIFAKAGMSDTFATNAVPEKYGTLISYNWDENT
                     SKKATEFVTLRPSGAFASTVVNL"
BASE COUNT          285 a          279 c          265 g          283 t
ORIGIN      
        1 caacgtgatc agttggataa ggctgtactc tggtggaaac gagcggtgac aactggtgat
       61 cagcttgacg caaactgtga tgtccaaacc caggaagcaa tccaaaaatt gattcgccgc
      121 attggggaac gattaattcg agaacaaaag accggtagcg tttcgttccg ttggacaatg
      181 tttcacgccc atcttcgcaa agacagttca attctccgcg aagctatctt gaagttccca
      241 gccttaccaa ccattgatcc gaaggaagct gagagccaga cccaggaatg agcttcgttt
      301 tccttcgaac gaacgtcttt tctgagcacc tcattatgaa accaaactcc tcccgtttct
      361 ctctgaattt gaattttgca cggtggtccg tgctgatttt gacattgtgc ctgtttacct
      421 tgcctcttcg ggccgatgaa attgacgatc tggtcaaaca acagatggaa gccgatcata
      481 ttccagggct ttcgattgcc gtgattcaca agggaaaaat tgtgaagctg caagggtacg
      541 gtgtctctga ccttgagaaa aaaactcctg ttacgccgca caccatgttc aaaatcgcga
      601 gtgtgagcaa acaatttatt gcgtccgcga ttgttctgct ggcagcagat ggcaaactca
      661 acctgaatga ttcgatgacc agatattttc ccgatgctcc ggaaacctgg aagccaatta
      721 cgctcaagca tttgctgagt catacttcgg ggctggtccg cgaatcgccg gcgtttcatc
      781 ccatctttgc caaatccgat ttggaaattg tcaaagcgac ctatcccgta gcgttgaagt
      841 ttcagcccgg tgaacggtgg gaatattgca acgtcggcta ttttgccctg gctgatatca
      901 tccgtcaggt cagcggcaag ccctggcccg agttttgcca ggaacgcatt tttgccaaag
      961 ccggaatgtc cgataccttc gcgaccaatg ccgttccgga aaaatacggc accctgatca
     1021 gttacaattg ggatgaaaat acctcaaaaa aagcgaccga atttgtcacg ctccgaccca
     1081 gtggggcgtt tgcttcaacg gtggttaatc tg
//
LOCUS       DAOQDO010002443         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_176292_length_1112_cov_2.919584, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002443 DAOQDO010000000
VERSION     DAOQDO010002443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176292_length_1112_cov_2.919584"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..>1112)
                     /locus_tag="PKZ53_27865"
     CDS             complement(94..>1112)
                     /locus_tag="PKZ53_27865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44330.1"
                     /translation="AQVRAYFDGQTGLLRRLESGEGATRRQMDLSEYRLVDGIQEPSV
                     IVLREGDVVTRLQIETVRHNTGLSASHFNIPKAADDLDLQTLFLQVDQNQTAIDDRVT
                     NYTYLLTETRRDLEKNGQVKETTVKVYEVYPLPGRERVRKLVSVNNKPLSAKDAEEEQ
                     KRVAEFIRDNEKRQEKKRTGKSEANDDARKRSLGISNFLKVCEFINPRRESLRGRTVV
                     VCDFRPRKGYKPTNDTEKIVAKLAGTVWIDETDKVVNRLEGRFDDDFKVAGGLLASLK
                     KGAAFTFDQTRTEEGVWLPLREDFNAGIKLFLFAGLNVSVENRYSDYKRFSVTTKIDG
                     EETQ"
BASE COUNT          234 a          301 c          253 g          324 t
ORIGIN      
        1 tcagggtttc gaactttggt cctttttgtt cttttcgtcc ctttcgtccc tttcttgttt
       61 acccgacctc ggaacccgaa cccggaaccc gattcactgt gtttcctccc cgtcaatttt
      121 cgtggtaact gaaaagcgct tgtaatcact gtatcggttc tcaacgctga cattcaatcc
      181 agcaaacagg aaaagcttga ttccggcgtt gaaatcttct cgaagcggaa gccagacccc
      241 ttcttcagtc cgggtctggt caaaggtaaa ggcagcgccc tttttcagag aagcaagcag
      301 cccacccgcg actttaaagt catcatcaaa gcggccttcg agccgattga ccactttgtc
      361 agtttcatca atccagacgg ttccagccag tttggccaca attttttcag tatcattagt
      421 gggtttgtac cctttgcgtg gcctgaaatc acacaccacg acggttcgac cgcgcaggga
      481 ttcacgccgt ggattaataa actcacatac cttcagaaaa tttgaaatcc ccagtgatcg
      541 cttcctggca tcgtcgttgg cttcggattt cccggttctc tttttttcct gccgtttttc
      601 attgtcgcgg ataaattcgg ccacacgttt ttgttcttcc tcggcgtctt tggctgagag
      661 tggtttgttg ttgacgctta ccagtttacg gacccgctct cgaccaggta gcgggtacac
      721 ttcatacact ttgacggtgg tttctttcac ctggccgttt ttttcaaggt cgcggcgggt
      781 ttcagtgagt aaataggtgt aattggtaac ccgatcatca atcgcggtct ggttttgatc
      841 aacttgaaga aacagtgttt gcagatcgag gtcatcggcg gcttttggaa tattgaaatg
      901 actcgctgaa agtccggtat tgtggcgcac tgtttcaatc tgcaatctgg ttacgacgtc
      961 cccttcccgg agcacgatca ccgatggttc ctggattcca tccacaaggc gatattctga
     1021 aagatccatc tgccgccggg tggcgccctc accgctttca agtcgccgca ataaaccagt
     1081 ttgaccatca aaataggccc gcacctgagc cc
//
LOCUS       DAOQDO010002444         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_176343_length_1112_cov_2.408704, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002444 DAOQDO010000000
VERSION     DAOQDO010002444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176343_length_1112_cov_2.408704"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="PKZ53_27870"
     CDS             <1..>1112
                     /locus_tag="PKZ53_27870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ44331.1"
                     /translation="KAPSVGVAVTLLNGILQGLEHLHERRIIHRDLKPANILLQGDIP
                     RLADFGIARVLKSTSSASMVSGTPLYMSPEAFDGKRSEQSDLWSVGVMAYQLLTGNFP
                     FPGGDFMAIVGKVAFAEPEPLSAFVPEPLQAVIYRALRKKPEERFRSAREMREALWKA
                     AYSVSLPGQSPSQAETVSATVSPTPVQKRTDTLPLAASHPNLPHPQPTQVVPGAIPTV
                     PAPPLAAQTAASNQLLASFPGTERVLMPEPKFPVGLLFGVAAIILVSLGLVVGGSLWV
                     FRDKLPAFSLSGVTPIGSSPVSSPSQPLGGQTAGGISELKLNGSFDNIIDMDFSADAS
                     KVVCGNFNGTLTLFDLTINQKLIELDDDQKTLSCVK"
BASE COUNT          256 a          308 c          286 g          262 t
ORIGIN      
        1 gaaagcgcct tcggttggcg tcgccgtgac cttgctcaac ggcattttgc agggactgga
       61 gcaccttcac gaacggcgca tcattcaccg cgatctcaaa ccagccaata ttttattgca
      121 gggcgacatt ccccgcctgg cggatttcgg aattgcccga gtgctcaaat caaccagttc
      181 ggccagtatg gtttcgggga cgccgctcta tatgtcaccc gaagcctttg acggaaaacg
      241 gtcggaacaa tcggatttgt ggtcagtcgg agtgatggcc taccagttat tgaccgggaa
      301 tttcccattt cctggtggag actttatggc aatcgtcggc aaagttgcgt ttgccgaacc
      361 ggaaccgctc tcagcatttg tacccgaacc gttacaggcc gttatttacc gtgccttgag
      421 aaaaaaaccg gaggagcggt ttcgctcggc tcgcgaaatg cgcgaagcgt tgtggaaagc
      481 ggcctattca gtctcattgc cggggcaatc tccttcccag gcagaaacag tgtcggcaac
      541 agtatctccg acaccagttc agaaacgaac tgatacttta cccctggctg cctcacaccc
      601 gaatctccca catccacagc caacccaggt ggttcccggc gcgataccta ccgttccggc
      661 accgccatta gccgcgcaga cggcagcctc gaatcagctt ttggcgagtt tccctggcac
      721 ggaacgagtg ttgatgccgg agccaaaatt tccagtgggg ttgctctttg gtgtggcagc
      781 catcattttg gtgagtttag gtctggttgt tggaggttcg ctctgggttt tcagggacaa
      841 gctccctgcc ttcagcctgt caggagtcac gccaatcgga tcatcgccag tttcctcgcc
      901 atcccagcca ttaggtggac aaacggcggg aggcatcagt gaactcaaac tcaacggcag
      961 ctttgacaac attattgaca tggatttctc cgccgatgcc agcaaagttg tatgcggaaa
     1021 tttcaatgga acattgacac tctttgattt gacgatcaac cagaagctaa ttgaactcga
     1081 cgacgatcaa aaaacactgt cctgcgtcaa aa
//
LOCUS       DAOQDO010002445         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_176394_length_1112_cov_2.131504, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002445 DAOQDO010000000
VERSION     DAOQDO010002445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176394_length_1112_cov_2.131504"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKZ53_27875"
     CDS             complement(<1..1076)
                     /locus_tag="PKZ53_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNJ44332.1"
                     /translation="MAARNIISGNGPGSVPGFGLYPGINIQNGGNNQIQGNAIGTDLS
                     GTTPLPNFGNGIYISSGQNNSVGGALPGAGNLIAFNYGNGVEIGHPAQSLSTSNTLVS
                     RNSIYSNTLFGINLVKTNNDGLTVNDLGDADTGNNGLQNYPTITSVASSGGSTTIQGT
                     LNSRPNTTYQLEFFANDAVTSFQYEGQEVIGTTSVTTDGAGNAAFSFTAPVTVATSRF
                     VTSTATDPLGNTSEFSFALKVNTAGNSQPPVIAAIYLNPPQAPAPASVNFNGGAFSAD
                     LDGTIVSYAWNFGDNTTGSGSVVAHTYLTAGNYPVTLTVTDNAGNTALTSVTVIVLAP
                     TAPPPPPPDPNVIPAPSNLTATAAS"
BASE COUNT          264 a          270 c          298 g          280 t
ORIGIN      
        1 gatgcagccg tggcggtcag gttcgagggt gccggaatga catttggatc cggtggcggt
       61 ggcggtggcg cggttggtgc cagaacaata accgtcactg aggtcaaagc cgtatttcca
      121 gcgttatcgg tgaccgtgag cgtgaccgga tagttccctg cagttaagta ggtatgtgcc
      181 acgacgcttc cagagccagt tgtattatcg ccaaagttcc aggcgtagga aacaatcgtc
      241 ccatccaggt cagccgaaaa cgccccgcca ttaaaattta cacttgctgg agccggagct
      301 tgtggtggat tcagataaat cgctgcaatg acaggcggct gcgaattccc ggcggtgttg
      361 accttcaagg caaatgaaaa ttcagacgta tttcccaacg ggtcggtggc cgttgaggtc
      421 acaaacctgg acgtggcaac ggtaactggt gctgtgaatg aaaaagccgc attccctgcc
      481 ccatcggtgg tcaccgaggt tgtaccaatc acttcctggc cttcatattg aaatgaagtc
      541 acggcatcgt tggcaaaaaa ctcaagctgg taggtggtat tgggacgact gttgagtgtt
      601 ccctgaatgg tggttgagcc accacttgag gcgacactgg tgatggttgg gtagttttgc
      661 aacccattgt tccccgtgtc ggcatctccc agatcattga ccgtcaatcc gtcattgttg
      721 gttttgacca ggttgattcc aaacagggta tttgagtaaa tcgaatttcg ggaaacaaga
      781 gtattggaag ttgatagaga ttgagccgga tgtccaattt cgacgccatt cccatagttg
      841 aacgcaatca gatttccggc tcctggcaga gcgcccccaa cgctattgtt ctgcccagac
      901 gagatataga taccatttcc aaaattcgga agaggcgttg ttccactcaa atccgtacca
      961 atggcatttc cctggatctg gttgttccct ccgttttgaa tgttaattcc tgggtacaag
     1021 ccaaaacccg gaacgctgcc tggaccattg cctgaaataa tattccgagc tgccacagtg
     1081 gttccgccaa tccggttatt tgatccagta at
//
LOCUS       DAOQDO010002446         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_176940_length_1110_cov_3.163033, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002446 DAOQDO010000000
VERSION     DAOQDO010002446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176940_length_1110_cov_3.163033"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PKZ53_27880"
     CDS             <1..997
                     /locus_tag="PKZ53_27880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ44333.1"
                     /translation="LNQAAQVLLEISAEAALGSVLEQVATGQLWEKAVSLVKTAAQTA
                     SPTASQVHTEQTGQTWDLTVNPVTTSPGNEEFHPMVILIIREITHLVELQESLRRSEV
                     MSKMGAVVGGVAHEVRNPLFSITAVLDAFEARFGAREEYQRYIQSLRKELTRMINLME
                     ELLEFGRPTNLTFAECAIQDVLNQAIQSCKPTAEKLKIELQVTVDTDFPAFRLDRRRL
                     AQVFHNLIENAIQHSPAGSAVLVEAKKIHINELPWLECVVKDRGPGFRAEDLPKLFDP
                     FFTRRRGGTGLGLSIVQRIVEDHGGKVLASNRVHGGAAMKVRIPLLKNEELRMKN"
BASE COUNT          256 a          292 c          289 g          273 t
ORIGIN      
        1 gctgaaccag gcagcccagg ttctgttgga aatcagcgct gaagccgccc tcggatcggt
       61 gctcgaacag gtagcgacgg gtcaactctg ggaaaaagca gtttccctgg ttaaaacggc
      121 tgcccagacg gcttccccga ctgcatctca ggtccatact gaacaaactg gccaaacctg
      181 ggatctgacc gttaacccgg tcacaacctc gcctggaaat gaagagtttc atccaatggt
      241 gattctgatt atccgtgaaa tcacccacct ggttgaattg caggaatcac tccgccggag
      301 tgaagttatg tcgaaaatgg gggcggtggt tggcggcgtc gcccacgaag tccggaaccc
      361 gctctttagc atcacggcgg tcctcgacgc ctttgaagcc cgatttggtg cgcgtgaaga
      421 ataccagcgc tatattcaat cgctgcgcaa agaactcacc cgcatgatca atttgatgga
      481 agaactgctt gaatttggcc ggccaaccaa cctcaccttt gccgaatgtg ccattcagga
      541 tgtattgaac caggccatcc aatcctgtaa accaacggct gaaaagttga aaatcgaact
      601 tcaagtcacc gtggacactg attttccagc ctttcgattg gatcgcagac gactggccca
      661 ggtctttcat aatctgatcg aaaacgccat ccagcattct cccgccggta gtgcggtcct
      721 ggttgaagca aaaaaaattc acatcaacga gcttccctgg ctagaatgcg ttgtcaaaga
      781 ccggggccct ggatttcgcg ctgaagacct gcccaagctc tttgacccct ttttcacccg
      841 ccggcgaggc ggcactggcc tcggactttc cattgtccag cgcatcgttg aagatcatgg
      901 cggaaaagtc ctggccagca accgcgttca cggcggcgca gcgatgaaag tcagaattcc
      961 cctgctcaag aatgaagaat taagaatgaa gaattgagaa tgcggttagt ggttagtggt
     1021 tagtttttgg tgcttggttc ttggtccttg gttcctggtt cttggtgctt ggttcttggt
     1081 tcttggttct tggttcttgg ttcttggttc
//
LOCUS       DAOQDO010002447         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_177269_length_1109_cov_2.821632, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002447 DAOQDO010000000
VERSION     DAOQDO010002447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177269_length_1109_cov_2.821632"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1109)
                     /locus_tag="PKZ53_27885"
     CDS             complement(<1..>1109)
                     /locus_tag="PKZ53_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HNJ44334.1"
                     /translation="MAGERHYLTSTRLAHPEFARELSKAPAFAALPADVRATILQQFH
                     ANTQEFFPGITEDTMPGELSNCIAGRVANLFNLHGPNYVCDAACASAMAAMSAAIEGL
                     VERDFDTVITGGIDRNMGIATFVKFCKIGALSATGTRPYADGSDGFVMGEGAAIFVLK
                     RLEDAERDGDTIYAVLRGMGGSSDGRGKGITAPNPIGQRLAIERGWKNAGISPATATL
                     IEGHGTSTKVGDVVEVQSLIDVFTPFGPATQSIALGSVKSNIGHLKGAAGAAGMLKAV
                     LALHHKVLPPSLNFHKPNPNLDFTHSPFYVNTELKPWPTPACGVRRAAVSAFGFGGTN
                     FHGVFEEYIPGALKPKKIQVSGIETVPAPGATQSPA"
BASE COUNT          242 a          297 c          321 g          249 t
ORIGIN      
        1 gctggcgatt gagtcgcccc tggtgccggt acagtttcaa tcccggaaac ctggattttc
       61 ttcggtttca gtgcccctgg aatgtattct tcaaaaacac cgtggaagtt ggtcccgcca
      121 aacccgaagg cgctgacggc ggcccgtcga acaccgcacg ctggcgttgg ccagggcttg
      181 agttcggtat tgacataaaa tggcgaatgg gtgaaatcaa gatttggatt tggtttatgg
      241 aagttgaggc ttggcggcag tactttgtga tgcaatgcca gaacggcctt taacatccct
      301 gcggcaccgg cagcgccttt gaggtggccg atattggatt tgactgaacc cagggcgatg
      361 gattgggtgg ctggtccaaa cggggtaaag acatcaatca ggctttggac ttcgaccacg
      421 tcaccaactt tggttgacgt cccgtggccc tcgatcaatg tggcggtcgc tggcgagatc
      481 ccggcatttt tccagccgcg ttcgattgcc agtcgctggc cgattggatt tggagccgtg
      541 atgccttttc cacgcccatc gctggagccg cccattccgc gcaggacagc ataaatcgta
      601 tcgccatcgc gttcggcgtc ttcaaggcgt ttgaggacaa aaatggccgc tccttcaccc
      661 atgacaaatc catcagagcc atcggcataa ggccgtgtcc ccgtcgctga cagggcgccg
      721 atcttgcaga atttcacaaa cgtggcaatg cccatgttgc ggtcaatccc gccagtaatc
      781 acggtgtcga aatcgcgttc aaccagaccc tcaatcgcgg cactcatggc tgccatggct
      841 gaagcacagg cggcgtcaca cacataattt ggaccgtgga ggttaaacag attggcgacg
      901 cgacccgcaa tacaattgga aagttcgcct ggcatcgtgt cttcagtgat accagggaag
      961 aattcctgag tattggcgtg aaattgctgg aggattgtgg cgcgaacatc ggctggaagc
     1021 gccgcaaacg ccggagcttt gctcaactcg cgggcaaatt caggatgggc aagccgggtt
     1081 gacgtcaagt agtgacgttc accggccat
//
LOCUS       DAOQDO010002448         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_177302_length_1109_cov_2.462049, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002448 DAOQDO010000000
VERSION     DAOQDO010002448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177302_length_1109_cov_2.462049"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKZ53_27890"
     CDS             <1..189
                     /locus_tag="PKZ53_27890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44335.1"
                     /translation="YTCCELDTFRQSRYLKSSLNEVQDSELVNQRNLAATHRHYIVAT
                     YDHVLEVVANNYEFKILD"
     gene            complement(303..860)
                     /locus_tag="PKZ53_27895"
     CDS             complement(303..860)
                     /locus_tag="PKZ53_27895"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNJ44336.1"
                     /translation="MAKEYANIPFNQVRRRDRAVEDEAWIEQLLEKVPFGSLSTLFNG
                     QPFINTNIFVYDRELHCIFTHTAREGRTRFNIEGDERVCFSVSEMGRLLPATTALEMS
                     VEYAGVVIFGRAHVVTDQVEATHGLQLLLDKYFPHLKPGEDYRHITEDELARTAVYRI
                     DIDQWSGKKKEVAADFPGAFLYGTR"
BASE COUNT          295 a          289 c          230 g          295 t
ORIGIN      
        1 tacacctgct gtgaactgga tactttccgt caatcaagat atctgaaatc cagtttgaac
       61 gaagtccagg actccgaact ggtcaatcaa cgaaatctgg cagcaaccca caggcattat
      121 atcgttgcca cctatgacca cgtgcttgaa gttgttgcca acaactatga gttcaaaatc
      181 ctggactgat tccctcgtac agccggcccc ggccaaactg gctttgaatc cagaaattgg
      241 actgagaacc agttacccca acaagtttcg gaatgggccc taactaccag gcaccaggaa
      301 cattacctgg tgccatacaa gaaagcgcca ggaaaatctg cggcgacttc cttctttttc
      361 ccactccact gatcaatatc aatccgatag acagcggttc gagccagctc atcctcagtg
      421 atgtgacggt aatcttcacc cggcttgaga tgaggaaaat atttatcaag caggagttgc
      481 agaccgtgtg tggcttcgac ctggtctgtc acgacgtgtg cccgcccaaa aatcaccacg
      541 cccgcatatt caacactcat ctccaaagca gttgtagcag gcagcaagcg acccatctcc
      601 gacacgctaa agcagacgcg ttcatcacct tcgatattaa accgcgtccg cccttcgcga
      661 gctgtatgtg tgaaaataca atgcaattca cggtcataga caaaaatatt tgtattgata
      721 aaaggctgcc cattaaaaag agttgacagc gagccaaaag gaactttttc aagtaattgc
      781 tcaatccacg cttcatcttc aacagcccga tcccggcgac gaacttgatt aaaaggaatg
      841 tttgcatatt ctttcgccat attatctcct caaccaaaac caaaaatctt caggtacgtc
      901 ttctgaaaca caggccaagt atgtctcaaa atggccttga cacaagtgcc aattttgacc
      961 acagtgatag agccaatttt tgaatgaaga atgtctcttg gttcctggtt cctggttctt
     1021 ggttcctggt tcctggttct tggttcctgg ttcctggttc ctggttcttg gttcttggtt
     1081 cttggttctt ggttcttggt tcttggttc
//
LOCUS       DAOQDO010002449         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178072_length_1107_cov_1.750951, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002449 DAOQDO010000000
VERSION     DAOQDO010002449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178072_length_1107_cov_1.750951"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKZ53_27900"
     CDS             <1..494
                     /locus_tag="PKZ53_27900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44337.1"
                     /translation="IFNADSAGGLKMIYPNPYLQNGENEILAHVPYQVPSIKEPDPDN
                     QWFYFLTEGPLREELYFVVSRTPQPGIPTGVQLIKYARKEHTNAWSPSDQIWQRIELA
                     RQTPLRQSTIDDFGSPQTEGELLAVTREIGLKPQDTPPSAIFMNSTAEAPLLVTRVQL
                     VKR"
     gene            642..>1107
                     /locus_tag="PKZ53_27905"
     CDS             642..>1107
                     /locus_tag="PKZ53_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNJ44338.1"
                     /translation="MDLDITELLIAWNQGDKDALEKLMPVVYGELHRIAKRSMYREQV
                     GHTLQPTALVNEVYMKLINQRRANWQNRAHFFAIAAQMMRRILVDHVRGQMREKRISR
                     NQTISLDIVSELPVDQPEVDVVALDTALDKLEALAPRQCRIVELRFFSGLNVD"
BASE COUNT          287 a          292 c          274 g          254 t
ORIGIN      
        1 atattttcaa cgctgattcg gcgggcggat taaaaatgat ctaccccaac ccatacctgc
       61 aaaatgggga aaatgaaatc ctggcccatg tgccctacca ggtgccgtca atcaaagaac
      121 cagatcctga caaccagtgg ttttattttc taactgaagg ccccttgcgc gaagaactct
      181 attttgtcgt cagccggaca cctcagccag ggattccaac tggagttcaa ttgatcaaat
      241 atgcccgtaa agagcacacc aacgcctggt cgccctccga tcagatctgg cagcgcattg
      301 aactggcccg gcagacacca ctccggcaga gcacaattga tgattttgga agcccccaaa
      361 ccgaaggcga attgctggcg gtgacccgtg aaatcgggct caaaccacaa gataccccgc
      421 cgtcagctat ttttatgaat tcgactgccg aagctccctt gctggtcacc cgagtccaac
      481 tggtcaaacg gtgaaacagg gatgaagatt gttaacctgt tttcttcagc ccggagtttt
      541 cagccccaag ccccaggccc taaacccaaa actgtgctcg aattgccaat ctgggctgtc
      601 ttccctatga tttccaaaga tttgaaggga ggatctggcg catggatctc gatattactg
      661 aattattgat tgcctggaat caaggtgaca aagacgcact cgaaaagctc atgccggtgg
      721 tctatggcga attgcaccgg attgccaaac ggtcaatgta tcgggagcag gtcgggcata
      781 cgctgcaacc cacagcgttg gtcaatgaag tatatatgaa attgatcaac cagcgccggg
      841 ccaactggca aaaccgggcg catttttttg ccattgccgc ccagatgatg cgccgaattc
      901 tggtagatca cgtgcgtggt caaatgcggg aaaaacggat tagtcgaaat cagacgattt
      961 cgctcgatat cgtctctgaa ttgccagttg accagcctga ggtagatgtg gttgcgctcg
     1021 atacagccct ggataaactc gaagccctgg cgccgcgcca gtgccggatt gtcgaacttc
     1081 gattctttag cggactcaat gttgatg
//
LOCUS       DAOQDO010002450         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178182_length_1106_cov_4.748811, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002450 DAOQDO010000000
VERSION     DAOQDO010002450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178182_length_1106_cov_4.748811"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..1031
                     /locus_tag="PKZ53_27910"
     CDS             477..1031
                     /locus_tag="PKZ53_27910"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ44339.1"
                     /translation="MKPLSINKDQHYIGTSELASAAAHQIRTLVPEQARGSVTEIPDE
                     RMVRYYITQRVLPEPLGKNGTSSVYGYRHLLVLLVVKRLQAEFLPLTKIRSIIAESTV
                     EDLERILTPKTSRETTQEFLESLLKSPSNPTGTPPSRTKLWQRHEIEPGFEIQVRQDW
                     VMPTDTESQLKLAQKILKKLSAEL"
BASE COUNT          339 a          266 c          227 g          274 t
ORIGIN      
        1 ctaaaataac taggtttgag gctgagaata tctaggtctg aggctgagaa gtgctaaaat
       61 aactaggttt gaggctgaga agttttacac aaaacattga ttcttaacag cttaccctgt
      121 ggaaaactcc cttaaccttt ttaaccatat atttaaccat taataatcat ggccccctag
      181 agatccgcca acgcaatgca acgtacaggg aaaaaggata ccgaaaataa aatgtggatc
      241 gcgcgattct cttaagtccc acttatcaaa atttttttga gaaatagaat aaaaccctct
      301 tcttactttc aaactggttc acttcaaacc tgtgacctga atgctttggc ccacgacaca
      361 ctcgaaagtg agtacctcaa tctggcaaga cgaaacaaat gaagcttcat caacacaaga
      421 tagttttttt cttaaacact attgacagta tttactgata agactaattt tcaaaaatga
      481 aaccactttc gatcaacaaa gatcagcact atatcggaac ctcagagtta gcttcggcgg
      541 cggcccacca aattcgaact ttggtgccag aacaggcacg gggctcggtg accgagatcc
      601 ccgacgaacg aatggtccgc tattacatca cccaacgggt tctgcctgaa ccgctcggga
      661 aaaacggcac gtcctcggtt tatggctatc ggcatttgct ggtgttgctg gtggtcaaac
      721 gactccaggc tgagtttctc ccgctgacca aaatccgaag catcatcgct gaatccacgg
      781 ttgaggatct ggaacggatc ttaaccccta aaacttccag ggaaaccacc caggaatttc
      841 tggaatcgct gttaaaatct ccgtcgaatc ccactgggac acccccttca cgcaccaaac
      901 tgtggcagcg gcatgaaatt gagcccggat ttgagattca ggtcagacaa gactgggtga
      961 tgcccaccga tactgaatca caactcaagc tggctcagaa aattctgaag aaactgtccg
     1021 ctgagttgta actgcctcaa tgacttgaaa aggatgttta tgcgtcaacc attgatagaa
     1081 attactcccg atcaaagtgt gtatga
//
LOCUS       DAOQDO010002451         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178202_length_1106_cov_3.307326, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002451 DAOQDO010000000
VERSION     DAOQDO010002451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178202_length_1106_cov_3.307326"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1106
                     /gene="mutS"
                     /locus_tag="PKZ53_27915"
     CDS             <1..>1106
                     /gene="mutS"
                     /locus_tag="PKZ53_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNJ44340.1"
                     /translation="GERFITPELKDYEEKILSAEQNILQLETTLFTELKQRIAAQIGR
                     IQITARILATLDVLGSFAQLAAMRNYVRPELHEDDELTILNGRHPVVETHTTRFVPND
                     VHLNNTTDRLVIITGPNMGGKSVYLRQTALIVILAQIGCFVPASQARLPLLDRVFTRV
                     GASDNLARGRSTFMVEMTEAANILNTATPRSLVLLDEIGRGTATFDGLSLAWAIAEHL
                     HNSPQHAAKTLFATHYHEMVELEKLLPGVHNVQLAVTAKDGHLIFLHKVVQGSASKSY
                     GIEVAKLAGLPAVVLSRAREILENLEANELDVLGKPKLARHMPSRKDWKKNQPTLFDK
                     ANESVIDDLRALDAEHLTPEEAKKALIALQAQLV"
BASE COUNT          289 a          293 c          274 g          250 t
ORIGIN      
        1 ggcgagcgct ttatcacacc tgaactcaag gactatgaag aaaaaattct aagtgccgag
       61 caaaatattc tccagcttga aaccacactc tttaccgaac tgaaacaacg gattgcggct
      121 caaatcggac gaatccaaat cacagcccga attctggcaa cgctcgatgt cctcgggtct
      181 tttgcccaac tggcggcgat gcgcaactat gtcaggcccg aactgcacga agatgacgaa
      241 ctgacgattc tcaatggacg ccatccagtg gttgaaaccc acaccacacg ctttgtcccc
      301 aatgatgtgc atctcaacaa cacgactgac cggctggtga ttatcactgg accgaacatg
      361 ggcgggaaaa gcgtctattt gcggcaaacg gcattgattg tgattttggc gcaaattgga
      421 tgttttgtac cggcctcaca ggcccggctt ccgttgctgg atcgggtttt tacccgcgtt
      481 ggtgcgtctg acaatctggc gcgtgggcgg tcaactttta tggtcgaaat gaccgaagcc
      541 gccaatattt tgaataccgc cacgccgcgc agcctggttc tgctggatga aattggccgt
      601 ggcaccgcca cgtttgatgg actctcgctg gcctgggcca ttgccgaaca ccttcacaac
      661 agcccacaac acgccgcgaa aacacttttt gccacgcatt atcacgaaat ggtcgagctt
      721 gaaaaattgc ttccgggtgt tcacaacgtg cagcttgccg tgacagccaa agacggtcat
      781 ctcattttcc tgcataaagt tgtgcaagga agcgccagta aatcctatgg gattgaagtt
      841 gctaaactgg ctggactccc ggcagttgtg ttgagtcgag cacgggaaat tctggaaaat
      901 cttgaggcca atgaacttga tgttttgggg aaaccaaaac tggcccggca tatgccctct
      961 cgcaaagact ggaagaaaaa tcagccgacg ctctttgata aagccaacga gtctgtcatt
     1021 gacgacctgc gggcccttga tgccgaacac ctgacaccag aagaagcaaa aaaagccttg
     1081 attgcattgc aagcccagct tgtata
//
LOCUS       DAOQDO010002452         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178213_length_1106_cov_3.015224, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002452 DAOQDO010000000
VERSION     DAOQDO010002452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178213_length_1106_cov_3.015224"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKZ53_27920"
     CDS             complement(<1..882)
                     /locus_tag="PKZ53_27920"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNJ44341.1"
                     /translation="MSDLTVIGSELDCAPCGWLSVTDDGCVVNVNETLANLLGQAKSH
                     IVGQNLSGFLTVGSRVFYQTHLFPQLKLAGRLDEIYLDLRSANGDTLPMLINAVRTER
                     QGEPANDFILLLIQRREKFESQILQSKRAAEAAAQAAQVANTQLIEMQKELEANHLKL
                     LELDELKTRFTAMLVHDLKSPLTVVNMALQLVQEQHRNPTEHLAEMLDVSKKNIQKIV
                     KMVNQMLDVYRGESRETKVNRLELDPMALLSDCVESARLAAPPQMTITCTVRNKLPAL
                     WADGLQLERVFSNLLSNA"
     gene            complement(879..>1106)
                     /locus_tag="PKZ53_27925"
     CDS             complement(879..>1106)
                     /locus_tag="PKZ53_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ44342.1"
                     /translation="FFSDNRADLPRVKTPSLVLQCQDDLIAPLQVGKYVHRQLVNSRF
                     RVMDATGHCPHVSHPQETIQSIRTYLNSQGV"
BASE COUNT          280 a          266 c          270 g          290 t
ORIGIN      
        1 ggcattggat aacaaatttg aaaatacccg ttcaagctga agaccgtccg cccaaagcgc
       61 aggaagtttg tttctgacgg tacaggtaat ggtcatctgg ggaggagcag ccagccgggc
      121 tgattcaaca cagtcgctga gcaatgccat cggatcaagt tcgagccggt ttacttttgt
      181 ttcccgtgat tcaccacgat agacgtcaag catctgattg accatcttga cgattttttg
      241 gatgtttttc tttgaaacat cgagcatctc agccagatgt tcggttggat tccggtgctg
      301 ttcctgtacc agttggagtg ccatattcac aaccgtcaac ggtgatttta agtcatgaac
      361 aagcatggcg gtgaaccggg tcttcaattc atccagttca agtaacttta agtgatttgc
      421 ctccagttct ttctgcattt caatgagttg agtatttgct acctgagcag cctgggcggc
      481 ggcttcggca gcgcgtttgg attgcaagat ttggctctca aatttctcgc gacgttgaat
      541 cagcaacaaa ataaaatcat tcgctggttc accctggcgc tctgtccgaa ctgcattgat
      601 cagcatgggc agggtgtcac cgtttgctga ccgcaaatca aggtagattt catccaacct
      661 tcctgccagc tttaactggg gaaaaaggtg tgtctggtaa aaaacacgac tgcccactgt
      721 cagaaaacct gacaggttct ggccgacgat gtgtgatttc gcctgaccta acagattggc
      781 cagtgtttca ttgacattga ccacacaccc atcatcagtg actgacagcc agccacaggg
      841 tgcacaatca agttccgatc caatcacagt caagtcgctc atacaccctg gctattcaaa
      901 taagtgcgga tagattgaat cgtttcttgt ggatgactca cgtgggggca atggccagtg
      961 gcgtccatca ccctgaacct ggaattgacc aattgacgat gaacatattt gccaacttga
     1021 agcggggcga tcaggtcatc ctgacattgc aaaacgagtg aaggggtctt taccctgggc
     1081 aaatcagccc ggttatctga gaagaa
//
LOCUS       DAOQDO010002453         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178249_length_1106_cov_2.539486, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002453 DAOQDO010000000
VERSION     DAOQDO010002453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178249_length_1106_cov_2.539486"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1106)
                     /locus_tag="PKZ53_27930"
     CDS             complement(<1..>1106)
                     /locus_tag="PKZ53_27930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ44343.1"
                     /translation="GVPDARSLILGFGATQSGNAFSICYPDYADLRDQNTSFEGLAVF
                     RNLTVNLSQDGKTDRTWACLVSGNYLDVLHVKPLLGRSFLPEEDRTPSTHPVVLMSYN
                     LWQSRFHGNPDVVGSTLTINGKPFTVIGVTPKGFVGGIVAISQDLWIPMMMQESIRPG
                     KDLLTSRGNRWLQMIGRLKPGVSLPQAQANLKAISAHIAETNPTTNTNWTVALYPLTQ
                     SPEGASKILGPVLLLLMGIVSVVLLVACTNVANLFMIKATNRRKEISIRIALGASRWR
                     IINQLLVESMALSVISGVVGLFIAVWTAKSLLLMLPTQRLPIYLDCSIDQRVLLFTFG
                     VTLATGFLFGLFPALQATRLNQATTLKDEGSSVT"
BASE COUNT          280 a          278 c          311 g          237 t
ORIGIN      
        1 aaggtgaccg atgacccttc atccttgagc gtggtggctt gattgagtcg tgtcgcctgc
       61 agggcgggga ataacccaaa cagaaatcca gtggccaggg tgacgccaaa ggtaaataac
      121 aaaacccgct ggtcaatcga acaatccaaa taaatcggaa gccgttgggt gggtagcatc
      181 agcaacaagg attttgcggt ccagacggca ataaacaggc cgacaacacc ggaaatgact
      241 gacagcgcca tgctttctac cagaagctgg ttgataatcc gccaccgact ggcccccagg
      301 gcaatgcgga tgctgatttc cttgcgccga ttggtggcct tgatcataaa caggttcgca
      361 acattggtgc aggccaccag caggacaacg ctgacaatac ccatcagaag taacaggacc
      421 gggcccagaa tttttgaggc accttcgggt gattgagtca gtgggtagag cgccaccgtc
      481 cagttggtat tggtggttgg gttggtttca gcaatgtggg ctgaaatggc tttgaggttg
      541 gcctgggctt gcgggaggga aaccccaggt ttgagccgtc caatcatttg cagccagcga
      601 ttgccgcgac tggtcaagag atcttttcct ggtcggatgg attcctgcat catcatggga
      661 atccacaaat cctgcgaaat ggcgacaatt cctccgacaa accctttggg agtgacccca
      721 atcacggtga agggcttgcc gttgatggtc aaagttgaac caaccacgtc tggatttcca
      781 tgaaatctcg attgccagag gttataactc atcaggacaa ctggatgagt ggaaggcgtg
      841 cgatcttctt cgggtaaaaa tgaacggccc agcagcggtt tgacgtgcag tacatccagg
      901 taatttccac tgaccagaca ggcccaggtc cggtcggtct tgccatcctg gctgagattg
      961 acggttaagt tgcgaaaaac ggcgagtcct tcaaacgatg tgttctggtc gcgcaaatca
     1021 gcataatccg gatagcaaat cgaaaacgca ttgccgcttt gggtcgcgcc aaaccccaaa
     1081 atcaggctcc gggcgtcggg cacacc
//
LOCUS       DAOQDO010002454         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178638_length_1105_cov_2.115238, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002454 DAOQDO010000000
VERSION     DAOQDO010002454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178638_length_1105_cov_2.115238"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKZ53_27935"
     CDS             <1..206
                     /locus_tag="PKZ53_27935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44344.1"
                     /translation="VFGILAGVAGALMGVIGGLFGMMMGLIALPFKILFGWGHSGWGF
                     FWSPAFYISLVVLLVWMVGRNRK"
     gene            complement(207..>1105)
                     /locus_tag="PKZ53_27940"
     CDS             complement(207..>1105)
                     /locus_tag="PKZ53_27940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44345.1"
                     /translation="SFTGVVPANLLATRSVVLYDPAFTQEELNQTQQAFQQIGIDAVA
                     YFERDVVTAGQDVVNAYTTYFISRQIQHIVYLDKGTNGYGFRAGLFNQKSSLVDMGAL
                     WRVQQPKLTEMLRVVFQDSWQSQKKKNFLISESPETDILIDFIPGKRQELYAFDLKVD
                     QLAVPRFGNEEMDRQLAQFFQENYPLKYKLTDPGTDEKELRRSGCAYVLCFVRTRGVA
                     ARQVLGYDMNRRETAYASITFPAGQLQLKTIPAQTEVYKFYFRHIDNGNVFLGSKWDA
                     DENWLDALRNLVLGFKADAKLY"
BASE COUNT          271 a          266 c          264 g          304 t
ORIGIN      
        1 tggtatttgg cattttggcc ggagtagccg gtgcattgat gggcgtgatc ggcggcctat
       61 ttggtatgat gatgggtttg atcgccctac ccttcaaaat cctgtttggg tggggacatt
      121 ccggttgggg attcttttgg tcacccgcgt tttatatcag cctcgtggta ctgctggttt
      181 ggatggtcgg ccggaaccga aagtgatcag tatagctttg catcggcttt gaatccaagt
      241 acgaggttgc ggagggcatc aagccagttt tcatccgcat cccatttgct tcccaaaaat
      301 acgtttccgt tatcaatgtg cctgaaatag aacttatata cttctgtttg ggccggaatg
      361 gtttttagtt gaagctgacc cgccgggaaa gtgatcgagg cataagcagt ctcacgacga
      421 ttcatatcat atcccaacac ctggcgtgct gcgacacccc gggtacgcac aaaacacagc
      481 acataggcac aaccgcttcg ccggagttcc ttctcatctg tgcccggatc cgtgagtttg
      541 tacttaagcg gataattttc ctgaaagaac tgagccaact gcctgtccat ctcttcattg
      601 ccgaaccggg gtacagccaa ttgatccacc tttaaatcaa aggcataaag ttcctggcgc
      661 ttacccggga tgaaatcaat tagaatatcg gtttccgggg attcactgat caggaaattc
      721 tttttcttct gactctgcca actgtcctga aaaacaacac gaagcatttc agtgagtttg
      781 ggttgctgta cgcgccacag cgcacccata tcgaccaggc tggacttttg attaaatagc
      841 cccgcccgaa atccataccc attggttccc ttatcaagat aaactatgtg ttgaatctga
      901 cgactgatga aataggtggt ataagcattg accacgtctt ggcctgcggt aaccacatct
      961 cgttcaaaat atgcgacagc atcaatgcca atctgctgaa atgcctgttg tgtttgatta
     1021 agttcttctt gtgtgaaggc aggatcatac aacaccacag acctggtggc aagtaaattg
     1081 gcaggcacaa cacccgtgaa tgatt
//
LOCUS       DAOQDO010002455         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_178850_length_1104_cov_2.857960, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002455 DAOQDO010000000
VERSION     DAOQDO010002455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178850_length_1104_cov_2.857960"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="malQ"
                     /locus_tag="PKZ53_27945"
     CDS             complement(<1..908)
                     /gene="malQ"
                     /locus_tag="PKZ53_27945"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNJ44346.1"
                     /translation="MSFQRSSGILLHPTSLPGRYGIGELGDEAFAFVDFLEAAGQTLW
                     QVLPLGPTGFGDSPYACFSAFAGNPLLVSLDSLVTDGLLSEDDLNDYPQLGEDRVNFG
                     EVVKRKNALLTQAFQTFQKKPAPELAAGFEHFCAEEAAWLDDYALFRALKDHHQGKAW
                     NGWEKKYVIRERQALHEAHETLHDLVEAHKFAQYLFFKQWRKLKTYANDKGIKIIGDI
                     PIFVAYDSTDVWTYPDLFKLDADGNPTVVAGVPPDYFSETGQLWGNPLYNWENMKGIH
                     YHWWVARLQNLLRLVDIIRLDHFRGFA"
BASE COUNT          270 a          265 c          275 g          294 t
ORIGIN      
        1 gcaaaccctc ggaagtgatc aagccggata atatcaacca ggcgcagcag gttttgaagc
       61 cgggccaccc accagtgata atggatccct ttcatatttt cccagttata gagcgggttt
      121 ccccataact ggccagtttc actaaagtaa tctggtggga cacccgccac gacggttggg
      181 tttccatccg cgtcgagttt aaacaaatcc ggataggtcc agacgtcggt tgagtcatag
      241 gccacaaaaa ttggaatatc cccaatgatc ttaatgcctt tgtcgttggc ataagttttc
      301 agttttctcc actgtttaaa aaacagatat tgggcaaatt tgtgggcttc gaccaggtca
      361 tgcaatgttt cgtgggcttc gtggagcgcc tgccgctcgc ggatgacata ttttttctcc
      421 cagccgttcc aggcttttcc ctggtgatgg tctttgagcg cccggaagag ggcatagtca
      481 tcaagccagg cggcttcttc agcacaaaaa tgctcaaatc cggcggcgag ttctggggct
      541 ggtttttttt gaaaggtttg gaaggcttgg gtcagcaagg cgtttttgcg tttgacgact
      601 tcaccaaaat tcacccggtc ttcacccaac tgggggtagt cgttcagatc gtcttcagat
      661 aacagtccgt ctgtgaccag agagtcgagg ctcaccagga gcggattgcc ggcaaatgcc
      721 gaaaagcagg catagggcga atcaccaaac ccggttgggc caagcggcaa gacctgccag
      781 agggtttggc cggctgcctc cagaaaatca acaaatgcaa aggcttcgtc tccaagttca
      841 ccaattccgt agcgacccgg caacgaggtc ggatgcaaca aaattccact tgatcgttga
      901 aaactcacag aattctcctt gatgagaaaa ttataccagt ttgtagccag tagtcaggag
      961 ttagtagttc actaactcct tttaattgaa ttgcttgcct gttttctaat atgagggttt
     1021 ccttgcaaat tggtattacc gctggttctg aaaatggtct cgaaccacgg ttggttgttc
     1081 aataattcgt ttttgctgaa tgcc
//
LOCUS       DAOQDO010002456         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_179612_length_1102_cov_1.969436, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002456 DAOQDO010000000
VERSION     DAOQDO010002456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179612_length_1102_cov_1.969436"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKZ53_27950"
     CDS             <1..315
                     /locus_tag="PKZ53_27950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HNJ44347.1"
                     /translation="SVEVELQLEPIVPPSVLYHGTGHRTVELILKDGLLKMNRHHVHL
                     SAGIETARMVGSRHGKPVVFEVAAEVMHHNGFVFFCSANGVWLVESVPPQYLTQLNVQ
                     AG"
     gene            complement(583..>1102)
                     /locus_tag="PKZ53_27955"
     CDS             complement(583..>1102)
                     /locus_tag="PKZ53_27955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44348.1"
                     /translation="INEVLEVFVAPSSAQAVETLGTRSDPESAISVVAKLSALTDATG
                     DQALTYREYTQKVTEAKVAVESFIAKNSEFSEQQPLFMAMRKITDSYEMVSPIWAMRI
                     GADQRKSLSGESPEMVMVLNAYPELKLTEYNRDGRYPTDKVVAWVWSQIPTQIQAVRS
                     QIRQLRGSLEGS"
BASE COUNT          280 a          276 c          260 g          286 t
ORIGIN      
        1 agcgttgagg tggaattaca gcttgaacca attgttccgc catcggtttt gtaccacggc
       61 acgggccatc gaacggttga actgattctc aaggatggac ttctgaaaat gaatcgccac
      121 cacgtccacc tctccgccgg catagaaact gcccggatgg ttggcagccg gcacgggaaa
      181 ccagtggttt ttgaagtcgc tgctgaagta atgcaccaca atggatttgt ttttttctgc
      241 tctgccaatg gggtttggct ggttgaaagc gtgccgccac agtatctcac ccaactgaat
      301 gttcaggctg gataaagttt cctccaaaaa taaaaaggcc gcggcaagga atcacatctt
      361 gccgcggaac cacttgagag aggaggaatg tacactatgc cgagaacaga agaagccaaa
      421 acatctttca ccagaagggc ctgcgtcggg tgtttgggca gcaacttttc acgtcaccct
      481 ctcaaacgcg accagttacg aaaagttcca aatcgtctcc attttttttc gatatttttc
      541 cagttgaaaa attgaacatt caaaatattg aaaaatttag acttaacttc cctccagtga
      601 acctcgcaac tgccggatct gtgatcttac cgcctgaatt tgggttggaa tctgcgacca
      661 aacccacgcc accactttat cagttggata ccggccatca cggttatatt cggttaattt
      721 caattctgga taggcattca agaccattac catttcagga gactcgcctg acagggattt
      781 tcgctggtca gcaccaatgc gcatggccca aatcggtgag accatttcat agctgtcagt
      841 aattttccgc atggccataa agagcggttg ttgttcagaa aattcagaat ttttggcaat
      901 aaaggattca accgccactt tggcttcggt cactttctgg gtgtactcac gataggtcag
      961 cgcctggtcc cctgtggcat cggtcagtgc cgacaatttt gccaccactg aaatggctga
     1021 ttccgggtcg gatcgagttc ccagggtttc aaccgcctga gctgaacttg gcgccacgaa
     1081 gacttcgagg acttcgttga tc
//
LOCUS       DAOQDO010002457         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_180505_length_1099_cov_3.352490, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002457 DAOQDO010000000
VERSION     DAOQDO010002457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180505_length_1099_cov_3.352490"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PKZ53_27960"
     CDS             <1..1005
                     /locus_tag="PKZ53_27960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ44349.1"
                     /translation="MKRLFVRPGFRGKRVGYQLALAIIEEARRLGHRCLRLDTIPGMD
                     EAQALYRQLGFHEIPAYRYNPNEYTKFFEISLTDEPQNTPKPAAREKLEIGIIGDFQP
                     GYRKHETTSSAIVEAAQALGLSMNLTWFGTNYLAVAEKRVELAGCDGICAAPSSPYRN
                     LDGGLAAIQLAREAKIPFLATCGGFQHTLLEFARHVAHLEQAESGEYNPEAKVPVLNL
                     SACSPVDGNFRLSGKKKIRLSPHARVAKIYGQLEVEEEFACNYELNQQFQEQLTSAGL
                     KVTGTTEGGDVRVIELIEHPFFVATAYLPQWSSSEGAPHPLFTAFLNAARMTYLLNEE
                     "
BASE COUNT          272 a          261 c          293 g          273 t
ORIGIN      
        1 atgaagcgcc tgtttgtccg gcctggattt cgaggaaagc gtgtcggcta tcaactggcg
       61 ctggcaatta ttgaggaagc ccgacgattg gggcaccgtt gcctgcggct tgacacgatt
      121 ccgggaatgg acgaagccca ggctctctat cggcaactgg ggttccacga gatcccggcc
      181 tatcgctata accccaatga atacaccaaa ttttttgaaa tttctctgac cgatgaacct
      241 cagaacacac caaaacctgc tgctcgtgaa aaactggaaa tcggcatcat cggagatttt
      301 cagccgggct atcgaaaaca cgaaacgaca tcttcagcca tcgttgaagc cgcacaggct
      361 cttgggttga gtatgaacct gacctggttt ggcacaaact atctggcagt ggctgaaaaa
      421 cgagttgaac tggcgggttg tgatgggatt tgtgccgctc cgagcagtcc ctatcgcaat
      481 ctggatggtg ggttggcagc tattcaactg gctcgggaag ccaaaattcc gtttcttgcc
      541 acctgcggcg gttttcaaca tacgttgctg gagtttgccc gccatgttgc ccatctggaa
      601 caggccgaat ctggtgaata taacccggaa gccaaagtcc cagtgctcaa cctttcagcc
      661 tgctcacctg tggatggaaa cttcagactg tcgggaaaaa agaaaatccg cctatcaccg
      721 catgccagag tggccaaaat ctatggtcag cttgaagtgg aagaggaatt cgcctgtaac
      781 tatgaattaa atcaacagtt tcaggagcaa ctgaccagcg cgggattgaa ggtgactgga
      841 acaaccgaag gcggagacgt cagagtgatc gaattgattg agcatccatt ttttgtcgcg
      901 acggcttacc ttccacaatg gtcatcaagc gagggtgcac cgcacccgct ctttactgcg
      961 tttctaaacg ccgcgcgaat gacatacctt ttgaatgaag aataaagaaa atgcaggtgc
     1021 ttggtgattg gtacttggtg cttggtgttt agtgcttggt gcttagtgtt tagtgcttgg
     1081 tgcttagtgt ttagtgctt
//
LOCUS       DAOQDO010002458         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_180512_length_1099_cov_3.255747, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002458 DAOQDO010000000
VERSION     DAOQDO010002458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180512_length_1099_cov_3.255747"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..>1099
                     /locus_tag="PKZ53_27965"
     CDS             38..>1099
                     /locus_tag="PKZ53_27965"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HNJ44350.1"
                     /translation="MPTHRSVTLPLDEIFVRLTCDTEKTEFEPLKRRLEKSKLGIVET
                     PGFDNEFLEDWPLAEHLSAVVQPEDVDLSRIIAHHRQVVILGDPGSGKTTLMRFLAFH
                     FAREFRDHGPQGIVTDKDGQTYGAARFPILIRISTYAEELRLNHNTSFREFLAVPFGD
                     TGIELPVLKRIFDQVLQDGEALVLLDGLDEVTEFHDRSQRIASFVSAVSPRNHFVVTS
                     RIAGYRAARLSGDFQQFTLRKFDKPQIEAFLNQWCLAVEQFHYPDLAPSDVGRLAQQE
                     IDGILQAVHESRGVLELASNPLMLTILALIHRNGKRLPSRRIELYDLAVKTRLRDWQL
                     QRGVRIEHVVKEKDALLFLA"
BASE COUNT          273 a          238 c          297 g          291 t
ORIGIN      
        1 cgtgagatgg aatcgtaagc ttgaggcaca gggaattatg ccgacccatc gctcagtgac
       61 gctgccgctt gatgaaattt ttgtcagatt gacgtgtgat acagaaaaga cagagtttga
      121 gccgttgaaa cgaaggctgg aaaaatcaaa acttggaatt gtggagacgc ccggatttga
      181 taacgagttt ttagaggatt ggcccttagc tgaacacctg tcagcagtgg tgcaacccga
      241 agatgtggac ttatcacgta tcattgctca tcatcgtcag gtggtcattt tgggcgatcc
      301 cggttcgggg aagaccaccc tcatgcggtt tctggcattt cattttgccc gtgaatttcg
      361 agatcatggc ccacagggaa tcgtgactga taaagatggt caaacgtatg gggcagctcg
      421 ctttccgata ctgattcgca tttcgactta tgccgaggaa ctgcggctca atcacaacac
      481 gtcattcaga gagtttcttg ctgtaccatt tggggatact gggattgagt tgccggtttt
      541 gaaacgtatt tttgatcaag tactccagga tggtgaggct ctggtgctgc tagatggcct
      601 ggatgaggtc actgagtttc atgaccgttc acagcgcatt gcaagttttg tatcggctgt
      661 ttcacccagg aatcattttg tggtgaccag tcgaatcgct ggctatcgag ctgccagatt
      721 aagcggggac tttcaacaat tcaccttgcg gaaatttgac aagcctcaga ttgaagcgtt
      781 tctcaatcaa tggtgcctgg ccgtcgaaca atttcactat cccgatttgg caccgtctga
      841 tgtaggtcgg ctggctcaac aggaaataga tggaatcctg caggcggtgc atgaaagtcg
      901 aggcgtttta gagctggcat ccaatccatt gatgttaacc atcctggcgt tgattcaccg
      961 aaatggaaaa cggcttccaa gccgtcgaat tgaactctat gacttggccg taaaaacacg
     1021 gttgcgggac tggcagctcc agcgaggggt aagaatcgaa cacgtcgtaa aagaaaaaga
     1081 tgccctgttg tttttggcg
//
LOCUS       DAOQDO010002459         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_180519_length_1099_cov_3.069923, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002459 DAOQDO010000000
VERSION     DAOQDO010002459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180519_length_1099_cov_3.069923"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1099)
                     /locus_tag="PKZ53_27970"
     CDS             complement(<1..>1099)
                     /locus_tag="PKZ53_27970"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44351.1"
                     /translation="KTAPITLEAVQKLIPANAALVEYAVYRPFDAKTGDFGKPRYAAY
                     VLQGVGDRGQGIGIEKQGSGFRVPGSGSKAKRMQSPQDALSGPNSGSKEKDEGQKVEP
                     GTRNPEPGTLALKPGTLSWVDLGDAEPIDQAVAAFRQALSSSKKSTVTEVQSVSQTLD
                     KLVMKPVRKLVGSAHHLLISPDGALNLVPFSALVDEKGKFLVEDYTLTYLTSGRDLLR
                     LGVKIESHDPPLVMADPDYKEGNGPQLLGSPLPRLSRLVGARIEGEGIKAIFPKAQLK
                     MKSEATKAILKQAVRPELLHIATHGYFLKDAPPQSTADDLPQAQRSLARVSQDAETQR
                     EQRELNPLLRSMLFFAGANQGGSGDNDGVMTA"
BASE COUNT          224 a          322 c          260 g          293 t
ORIGIN      
        1 gtgccgtcat caccccatcg ttgtcaccac ttccgccctg attggcgccc gcaaaaaaca
       61 gcattgaccg taacaatggg tttaattcgc gctgctctcg ctgcgtttca gcgtcttgcg
      121 atactcttgc taaagatcgt tgagcctgag gtaaatcatc tgcggtggat tgaggtgggg
      181 catctttcag aaaatatcca tgcgtggcga tgtgcagcag ttccggcctg acggcttgct
      241 taagaatcgc ttttgtcgct tcggatttca tctttaattg tgccttggga aaaatcgctt
      301 tgatcccctc gccttcaata cgtgcgccca ccagtcgcga caatcggggc agtggacttc
      361 ccaacaattg tgggccattt ccttctttat aatctggatc agccatcacc agcggcgggt
      421 catggctttc aatcttgacg cccagtcgca gcaaatcacg tccgctggtc aggtaggtca
      481 gggtgtagtc ttccaccaga aacttccctt tttcatcaac cagcgctgaa aatggaacca
      541 gattcaaagc cccgtccggt gaaatcaaca gatgatgagc cgatccgacc agtttccgca
      601 ctggcttcat caccagtttg tccagggtct gtgacaccga ttgcacttct gtcacagtac
      661 ttttctttga gctactcaat gcctgccgga aggcagccac agcctggtca attggctcgg
      721 cgtcaccgag gtcaacccaa gacagggttc cgggttttaa agccagggtt ccgggttccg
      781 ggttccgggt tccgggctcc actttttgac cttcgtcttt ttcctttgaa cctgagttcg
      841 gccctgacaa agcatcttgc ggcgattgca ttctctttgc cttcgacccg gaacccggaa
      901 cccggaaccc ggacccctgt ttttcaattc ctatcccctg tcccctgtcc cctactccct
      961 gtaaaacata agccgcatac cgtggttttc caaagtctcc tgtcttcgca tcaaagggcc
     1021 gatacacggc atattccacc agtgccgcat tggcaggaat cagtttttga acggcttcca
     1081 gggtaatcgg ggcagtctt
//
LOCUS       DAOQDO010002460         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_180591_length_1099_cov_2.344828, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002460 DAOQDO010000000
VERSION     DAOQDO010002460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180591_length_1099_cov_2.344828"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKZ53_27975"
     CDS             <1..229
                     /locus_tag="PKZ53_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNJ44352.1"
                     /translation="LIEAADAAAKTADVRLLEIRLAMALGGKAFVTMTGTVAAVQAAV
                     SAGAAVLGAKGLLVNKVVIPRPRKELLTEMI"
     gene            278..>1099
                     /locus_tag="PKZ53_27980"
     CDS             278..>1099
                     /locus_tag="PKZ53_27980"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HNJ44353.1"
                     /translation="MKKRVSCLTWWLIGMAVFLLNGCWLVGETPPVELTTARSDFKVA
                     ILLPGPANDKAWNQSGYEGLKLIEQEFKAETAFVERLEGPELAVRSREAAHRFAASGF
                     DLVILHGGEFVPIAESVAREFPRTRFALSGTYAGNNSNLGAVAFRFEESGYLSGVLAA
                     LHTRSNKVAYIAGFDYPSYVEEGKLFEIGAKSIKPEIQVKIAYLQTWFDPNKARDTAL
                     GLARDGYDILLINADAPGLMAIREVTKLDGVYVIGHNQDQSDIAPGRVLTSVIDNV"
BASE COUNT          238 a          263 c          335 g          263 t
ORIGIN      
        1 gctgattgaa gccgccgatg ccgcagccaa aacggcggat gttcggttgc tcgaaatccg
       61 gctggcgatg gcgcttggag ggaaagcctt tgtgacgatg accgggacgg tggctgcggt
      121 gcaggcagca gtttcagccg gggccgccgt tttgggggcc aaaggactgc tggtcaataa
      181 agttgtcatt ccacggcctc ggaaagagct tttgaccgaa atgatatgaa ccgctgacct
      241 ggcggctcac tggggcagac tcagagggaa aactggaatg aagaaaagag tttcttgcct
      301 gacctggtgg ctgattggca tggccgtttt cttactgaac ggatgctggc tggtggggga
      361 aactccacct gtggaactga ccaccgcgcg gagtgacttc aaagttgcca ttttgttgcc
      421 cggacctgcc aacgataaag cctggaatca gtctggctat gaagggctca aactcatcga
      481 gcaagaattc aaagctgaaa cggcgtttgt tgagcggctt gaaggtcctg agcttgcagt
      541 tcgttcgcgt gaggccgccc atcggtttgc cgcatccggg tttgatctcg tgattttgca
      601 cggcggggag tttgtcccga ttgccgaaag cgtggctcgc gaatttcccc ggactcgatt
      661 tgcactctct ggaacctacg cgggaaataa cagcaacctg ggtgcggttg cttttcggtt
      721 tgaggaatcc ggatatcttt ccggagtgct tgctgccctg catactcggt ccaataaagt
      781 ggcttacatc gccgggtttg attatccaag ttatgtcgaa gagggcaaac tctttgagat
      841 tggcgccaaa tccatcaagc ctgagatcca ggtcaaaata gcctaccttc agacctggtt
      901 tgatccaaac aaggcccgag acaccgccct gggtctggcc cgagatgggt acgatatttt
      961 gttgatcaat gcggatgccc cagggctgat ggcgattcgt gaagtcacca aactggatgg
     1021 cgtgtatgtg attggccaca atcaggatca gtctgatatt gctccgggtc gggtgctgac
     1081 gagtgtgatt gacaatgtg
//
LOCUS       DAOQDO010002461         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_180636_length_1099_cov_2.050766, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002461 DAOQDO010000000
VERSION     DAOQDO010002461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180636_length_1099_cov_2.050766"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKZ53_27985"
     CDS             <1..670
                     /locus_tag="PKZ53_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015140054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44354.1"
                     /translation="QTDPQIGKTSTLTRAFESFDETRISFEGQQIRRLPGSLKEADTL
                     SELTAQSEKHISTGFEATRTAVLENDLTQFRYVHFATHGLLNSGSPELSGLVLTLVNS
                     DGTPQDGFLSLQDIYNLKLDSDCVVLSACQTALGKNMRGEGLVGLTRGFMYAGARRVV
                     ASLWSVNDASTVELMREFYGQILQKHQRPAAALRLAQQTLWKQKPNRNPYFWAGFILQ
                     GEYR"
     gene            686..>1099
                     /locus_tag="PKZ53_27990"
     CDS             686..>1099
                     /locus_tag="PKZ53_27990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44355.1"
                     /translation="MTDSLVASKGSKWELTYTSFRLLLQKLDPDPHVAGEKYERLRLK
                     LIWFFRDRRCLTPDEQTDKTLDRVAQKLLDGEDIENINAYVYTVARYIFQEYLRTNQP
                     DELNPEILQKPLYSTQTASSHDDPANQLDKEKVHSY"
BASE COUNT          301 a          270 c          252 g          276 t
ORIGIN      
        1 tcaaacagat ccccagatcg gcaaaacaag tacgctgacc agagcctttg agtcctttga
       61 cgaaacccga atttcatttg aaggacagca gattcgacgg ttacctggaa gcctcaagga
      121 ggcagatacc ctttcggagt taactgctca atcagagaaa cacatctcaa ctggttttga
      181 agcaacccgt acggcagtcc ttgaaaatga tttaacccag tttcgatatg tccattttgc
      241 aacccacgga cttttgaata gtggctctcc tgagctttcc gggttggtcc tgacgctggt
      301 aaattcagat ggaaccccgc aagatggatt tctctcgtta caagatatct ataatttgaa
      361 actagactct gattgtgtcg tgttgagtgc gtgtcaaacc gcccttggga agaatatgcg
      421 cggagaaggg ttggttggac ttacccgtgg ttttatgtat gctggagccc gccgggtcgt
      481 tgccagttta tggtctgtga acgatgccag caccgttgaa ctgatgcgcg agttttacgg
      541 gcaaattctc caaaaacacc agcgaccggc ggctgcactc cgactggcac agcaaacgct
      601 atggaaacag aaaccaaatc ggaatcctta tttttgggca ggctttattc tccagggtga
      661 ataccgctaa caggtggacg ttctcatgac tgattctttg gttgcatcga aaggttccaa
      721 atgggagctt acctatacca gcttcaggct gttgctccaa aagttagacc ctgatccaca
      781 cgtggcaggc gaaaaatatg aacgcctgcg actcaaattg atctggttct ttcgggaccg
      841 gcggtgtctt acacctgatg agcaaactga taaaaccctg gaccgtgttg cacaaaaact
      901 tctggacggt gaggacattg agaatatcaa tgcctatgtc tacaccgttg cccgatacat
      961 ttttcaagag tacctgcgaa ccaatcagcc agatgaactc aacccagaaa ttcttcaaaa
     1021 accactgtat tccactcaaa ctgcgtcaag tcacgatgac cctgccaatc aactcgacaa
     1081 agagaaagtt cattcctat
//
LOCUS       DAOQDO010002462         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_180910_length_1098_cov_2.533078, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002462 DAOQDO010000000
VERSION     DAOQDO010002462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180910_length_1098_cov_2.533078"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1098)
                     /locus_tag="PKZ53_27995"
     CDS             complement(<1..>1098)
                     /locus_tag="PKZ53_27995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44356.1"
                     /translation="KIHRANTRTLGCVDLLQIARTELKRNHLNAALAAIEESAALTEL
                     LRQTKPGQDFRALFQSTVHETYEVWVDILMALHRAHPKEGYQIKAFEISERSRMRSFL
                     DLAAESKKQLTQAPLRSLVEREQALRKRLAEQSEKLLRLSADEKTEATELKQNIEAIR
                     IQLATVQAEMNPAEKVHSALVVLASLQARLKNTDPVNTKSTTSLNLEIERTTRELNSL
                     KAEYQLENQKQDDEPQILSLAQIQANLLDPDTQVCAYLLGEEQSYAWIVTQTSLDTYV
                     LPKRELIEQAVDQYRHLMSGEVPSPTQSQDIAQAGQAVSKLLLEPVRHKFTGKRVLII
                     PDGALYTLPFAALPIGKGPKPDEPLLVRHEVV"
BASE COUNT          224 a          273 c          302 g          299 t
ORIGIN      
        1 accacttcgt gcctgaccag caatggttcg tcaggttttg gccccttgcc aatcggtaac
       61 gccgcaaacg gaagggtgta caacgccccg tccggaataa tcagcacccg cttgccggtg
      121 aatttgtggc gaactggttc caggagtaat ttcgacaccg cctgaccggc ctgggcaatg
      181 tcctgcgatt gagtcgggct gggaacttcg ccagacatca ggtgccgata ctggtccacc
      241 gcttgttcaa tcagttcgcg tttcggcaaa acataggtgt cgagtgaggt ctgggtcaca
      301 atccacgcat agctttgctc ttctcccaac aggtaagcac acacctgcgt gtcagggtcg
      361 agcaggttcg cttgaatttg ggccagagac aggatttgtg gttcatcgtc ttgcttctgg
      421 ttttccagtt gatattcagc tttgagtgag ttgagttcgc gggtggtccg ttcgatttcc
      481 aggttgagcg aagtcgtgga ttttgtattc accggatctg tatttttcag tcgtgcctgc
      541 aatgaggcca atacaaccaa cgccgaatgg actttttccg ccgggttcat ctcagcctga
      601 accgtagcca gttgaatgcg gatagcctca atattttgtt tgagttcggt ggcttcggtt
      661 ttttcatcgg ctgaaagccg gagcagtttt tcagattgtt cggccaaccg tttgcggagt
      721 gcctgctcgc gttccaccag tgagcggagc ggcgcctggg tcaattgttt tttggattca
      781 gccgccagat caagaaagct ccgcatgcgc gagcgttccg aaatttcaaa ggccttgatc
      841 tgatagcctt cctttggatg agcccgatgg agcgccatca aaatatcaac ccagacttcg
      901 taggtttcat ggaccgtgga ttggaacaac gcccggaaat cctgtcccgg cttggtttgg
      961 cgcaataact cagtcaatgc ggcggattct tcaatcgccg cgagggcagc gttgagatgg
     1021 tttcgtttta attccgttct ggcaatttga agaagatcaa cgcagccaag tgtacgggta
     1081 tttgctcgat ggatcttt
//
LOCUS       DAOQDO010002463         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_181190_length_1097_cov_3.160269, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002463 DAOQDO010000000
VERSION     DAOQDO010002463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181190_length_1097_cov_3.160269"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKZ53_28000"
     CDS             complement(<1..347)
                     /locus_tag="PKZ53_28000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44357.1"
                     /translation="MIKQKPTANPPVTDDAHSTAELTLSPHVANEPAAITVNDSKALM
                     PLVLERLRDFVNKSNSDATRRAYRVTMEQFQIFLRNRGKNMVQATPADVLAFRDGLIH
                     GPKPLKPASVTLRL"
     gene            570..>1097
                     /locus_tag="PKZ53_28005"
     CDS             570..>1097
                     /locus_tag="PKZ53_28005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44358.1"
                     /translation="MRFIQVTGIIILSCLCYLSGSHPAMAQTQSKNTKKNGAATSQQI
                     VPPSGLQTGPEHPTPSSLASLTVIQSPTDEVTIEEGKIYHLRVRPNFVCLVELPVLPE
                     AILVGNPARFLVEPLPETSAQPVKTAPPSSRLLLVKPLKINDPAGDRLVTNLTIKLAN
                     GRVVSILLDNTDSETL"
BASE COUNT          275 a          294 c          264 g          264 t
ORIGIN      
        1 agccgcaacg tgaccgaagc cggtttcaag ggctttggcc cgtggatgag cccatcccga
       61 aaggctaaaa catctgccgg cgtcgcctgc accatatttt ttccccggtt gcgaagaaaa
      121 atttgaaatt gctccatggt gacccgatag gcacggcggg tcgcatctga attcgatttg
      181 tttacaaaat cgcgaaggcg ctctaacacc agcggcatca gggcttttga atcattgacc
      241 gtgatagctg ccggctcatt tgctacgtgt ggtgacaggg tcaattcagc cgtcgagtga
      301 gcatcatcgg tgactggcgg gttcgcggtg ggtttttgtt tgatcataag tcagagagtg
      361 atggtttcag cttaccagca gcattcgcgt agatcggatt caaccaaaat gacttctgtt
      421 aactttcatt atcggaagta gactacgccg gatcaaccag ccatgtccag aggaatttcc
      481 agtcaccaga caatttaaaa ttcaatggaa caccagttgc ccttgaggat ttcgcttaaa
      541 aggcaatttt aattctgact ggagagccta tgcgttttat tcaggtgact ggaattatta
      601 tcttgagttg tttgtgctac ctgtcagggt cacacccggc aatggcccaa acacaatcca
      661 aaaatacgaa aaaaaatgga gctgccacct ctcaacagat agtcccccca tcgggtctcc
      721 agactggacc cgaacatccc acaccgtcct cgctcgccag cctcacggtc atccagtcac
      781 caacggatga agtgactatc gaggagggta agatttatca ccttcgggtc cgacccaatt
      841 ttgtgtgcct ggtggaattg ccggttctcc cagaagccat tctggtggga aacccggccc
      901 ggtttctggt cgaaccattg cccgagacgt cagcccagcc agtcaaaact gcccccccct
      961 cttcgcgcct gttactggta aaaccactca aaatcaatga cccagccggg gatcggctgg
     1021 ttaccaacct gacgatcaag ctcgccaatg gacgcgtggt atcaatctta ttggataata
     1081 ccgacagtga aacactc
//
LOCUS       DAOQDO010002464         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_181222_length_1097_cov_2.625720, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002464 DAOQDO010000000
VERSION     DAOQDO010002464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181222_length_1097_cov_2.625720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1097
                     /locus_tag="PKZ53_28010"
     CDS             <1..>1097
                     /locus_tag="PKZ53_28010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44359.1"
                     /translation="SSAVRRRDAKTIVVTSEGDGQREMVVSYTIAAPIWKTSYRVVMD
                     SSGKPFFQGWAIVDNISEEDWENVSLSLVSGTPMSFIQPLQQPLYRYRPVLPIANDVK
                     LDPQGVNPVDLSLADGDSPESETASTVTKRQVKELPSPASQGQLNLVQTQTGVYSVMN
                     AAPNDGTGRYYRSSTGPTTTLSTAITTGEAGITSTSTGNEIGDLFEYKVSQPVTVRRD
                     RSALVPILQEKLEGERVSLYNESINPERPLNGVRLLNSTKLTLESGILTVVDGDAYAG
                     EGSIDRCKPGERRFVVYALDLGTLVTTKTESEQKPVFLVRKYYGNFEIHYYLIEKKKF
                     TVTNQTDRPKTVYLEHPIRQGWTLTKDMGEP"
BASE COUNT          282 a          290 c          295 g          230 t
ORIGIN      
        1 cagttcggct gttcgtcgcc gcgatgccaa aacaatcgtg gtgacgtcag aaggtgacgg
       61 ccagcgggag atggtggtga gttacacaat tgccgccccg atttggaaaa catcctatcg
      121 ggtggtgatg gatagcagcg gaaagccctt tttccagggt tgggcgattg tggacaacat
      181 cagcgaagaa gactgggaaa acgtctcgct ctcgctggtt tcagggacgc cgatgtcatt
      241 tattcagccg ctccagcagc cgctctaccg ctatcgcccg gtgcttccga ttgccaacga
      301 tgtcaaactt gatccacaag gtgtcaaccc ggttgatctt tcactggcgg atggtgactc
      361 acccgaatct gagaccgcct caaccgtgac gaaaagacag gtgaaagaat tgccgtcgcc
      421 agcctcacaa ggtcaattaa atctggttca aacgcagact ggagtgtatt cagtgatgaa
      481 tgcggccccg aacgatggta ctggcaggta ctatcgttct tcaaccggcc cgaccacaac
      541 cctcagtaca gccatcacta cgggtgaggc cgggatcact tcgacctcaa ccggaaacga
      601 aattggggac ttatttgaat acaaagtttc ccaaccggtg acggttcggc gtgaccggtc
      661 agcactggtg ccgattctgc aggaaaaact cgaaggcgaa cgggtgtcgc tctacaacga
      721 atccatcaac ccggaacgcc cgctcaatgg ggttcggctg ctcaactcga ccaaattgac
      781 gcttgaaagt ggaatcctga cagtggtgga tggcgatgcc tacgccggag aaggcagcat
      841 tgaccgctgt aagccgggcg aacgccggtt tgtcgtctat gcgcttgatt tgggtacgct
      901 ggtcacgacc aaaaccgaat cagaacaaaa accggtgttt ctggtgcgga aatactacgg
      961 caattttgaa atccattact atctgattga aaagaagaaa tttaccgtca caaaccagac
     1021 ggaccgcccg aagacggttt atctcgaaca ccctatccgt cagggctgga ctttaaccaa
     1081 agacatgggc gaaccac
//
LOCUS       DAOQDO010002465         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_181555_length_1096_cov_2.509126, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002465 DAOQDO010000000
VERSION     DAOQDO010002465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181555_length_1096_cov_2.509126"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..>1096
                     /locus_tag="PKZ53_28015"
     CDS             4..>1096
                     /locus_tag="PKZ53_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44360.1"
                     /translation="MVAATSGEASLQVMRGDGFGGFAQPETVELSGTLISLTSTRNDS
                     SLSPTLVVGLTGEKTSSLLVYSGGLKATPHTVSLPLDEPTHLAISMPDQQGVSSLGAA
                     TGETLMLVRGLGAKALKIKRTFDHPVQGLAFGHFTQAEKTEVAVLVSNGDVFVFSVDA
                     LFADATAEGVLFGRIPNPKGLAGLSVGHFAKDAAGLDDLLVMDRGTRKVYVLVANDLL
                     NREDRTVTAEFDVSTRLVAVAPIQMTAGQPQELVVLSLGPNVSLVSQTKKEAVVVTSQ
                     DSSSDTTVFAPSEKRPDLEVNPLYSPGKDGALTVNTAATIVNLYTTLTADAAAGATSI
                     TVANTTGTNRLVPTTHLTAGDLILIIQMQG"
BASE COUNT          245 a          306 c          304 g          241 t
ORIGIN      
        1 gatgtggtgg ctgccacgag tggcgaagct tcgctccagg tgatgcgcgg cgatggtttt
       61 ggcggatttg cccaaccgga aacggtggaa ctttcgggca cacttatttc tctcacctca
      121 accaggaatg attccagcct gtcgccaaca ctggtggttg gtctgactgg cgaaaaaaca
      181 tcctccctgc tggtttactc aggcggtttg aaggcgactc cgcacactgt cagcctcccg
      241 ctcgatgaac ccacgcacct ggcaatatca atgccagacc agcagggggt atcttccctg
      301 ggtgcagcca ccggggaaac cctgatgctc gtcagaggat tgggggcgaa agccctcaag
      361 ataaaacgga catttgacca tccggtacag ggcctggcat ttgggcactt tacccaggcc
      421 gaaaaaaccg aagtcgccgt gctggtgagc aacggcgacg tttttgtgtt ttcagttgat
      481 gcgcttttcg cggatgccac agccgaaggc gtgctgtttg gtcgcattcc aaatccaaaa
      541 ggactggctg gcctgagcgt cgggcacttt gccaaagatg ctgctggcct tgatgattta
      601 ctggtgatgg atcgcggcac ccgaaaagtc tatgtactgg tcgccaatga cttgttgaat
      661 cgggaagacc gcacggtgac ggctgaattt gatgtgagca cccggctggt cgccgtggcg
      721 ccgatccaga tgactgccgg ccagccacag gaactggtcg tgctctcact tggtccaaac
      781 gtctcgctcg tttcgcagac gaaaaaagaa gcagtggttg tcacctctca agatagttct
      841 tctgacacaa ccgtttttgc cccaagtgaa aaacgtcctg atttagaggt caacccgctt
      901 tactccccag ggaaagacgg tgcgttgacg gtcaacacgg ctgccacaat cgtcaacctc
      961 tacaccactt tgacggcgga cgccgccgct ggagcgacca gtattacagt tgccaacaca
     1021 actggaacca accgacttgt tccgacaact cacttgacgg caggcgacct gattttgatc
     1081 atccagatgc agggcg
//
LOCUS       DAOQDO010002466         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_181622_length_1096_cov_2.029779, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002466 DAOQDO010000000
VERSION     DAOQDO010002466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181622_length_1096_cov_2.029779"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..523)
                     /locus_tag="PKZ53_28020"
     CDS             complement(95..523)
                     /locus_tag="PKZ53_28020"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNJ44361.1"
                     /translation="MKPRLLQKPETLSFHMILGNGIDIVEIERMAGALTQHGARFRNR
                     IFTDQEILYCDPKARAAQHYAGRFAAKEAAFKALGTGWSRGLSWHDVEVVSAINGPPT
                     LTLHGLALEIFQQKGGTRLHLSISHTERYAVAFVIFEGPG"
     gene            complement(549..>1096)
                     /locus_tag="PKZ53_28025"
     CDS             complement(549..>1096)
                     /locus_tag="PKZ53_28025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ44362.1"
                     /translation="LLDTAALEGKKLKLKKEPASLRSLAQFVIDDNRTHAAAKRITLH
                     FEADPNLDWTAEIDEARIQTVFDNLVSNALKYSPQGKEVWIWLEKREDQIVFSVRDEG
                     PGLSPEDQAKLFVKYQRLSAKPTGGESSTGLGLAIVKELIDLHGGKTWVESTLNHGAT
                     FRVELPIKLQPSEPPAEKVTR"
BASE COUNT          259 a          282 c          281 g          274 t
ORIGIN      
        1 aaccaagaac caagaaccaa gaaccaagaa ccaagaacca agaaccaagc accaaccaca
       61 ttcttcattc ttcattcttc attcttcatt caaatcatcc tggtccttcg aaaatcacaa
      121 aggcaacggc atagcgttcg gtgtgggaaa tggaaagatg aagtcgtgtt ccgccttttt
      181 gctggaaaat ctccagtgcc agaccatgca gggtcagcgt tggggggccg ttgatggcgg
      241 aaacaacctc cacatcatgc cagcttaatc cgcgtgacca gcccgtacca agggctttga
      301 aggccgcttc tttggctgca aatcgcccgg cataatgctg ggcagcccgg gctttgggat
      361 cgcaatagag aatttcctga tcggtaaaga tgcgattgcg aaaccgggcc ccgtgctgag
      421 tgagtgcacc cgccatccgt tcaatttcaa caatgtcgat tccgtttcct aaaatcatat
      481 gaaagctgag ggtttcgggt ttttgaagca ggcgcggttt caaacaacag cgccctgacc
      541 ccaggttgtc accttgtcac tttctcagcc ggtggttccg atggctgaag cttgattgga
      601 agttccacac ggaaggtcgc gccgtggttc agcgtgcttt caacccaggt tttaccaccg
      661 tgaagatcaa tcagttcttt cacaatcgcc aggccaagac cggttgaaga ctcaccccct
      721 gtcggcttgg cagagagtcg ctggtatttt acaaagagtt tggcctggtc ctctgggctc
      781 aaaccgggac cttcgtcgcg aactgaaaag acaatctggt cctcgcgttt ttcgagccag
      841 atccagactt ccttgccctg gggagaatat ttcagggcgt tgctcaccag attatcgaag
      901 actgtttgaa tacgagcttc gtcaatttca gcggtccaat cgaggtttgg gtcagcttca
      961 aagtggagtg taatgcgttt ggccgcggcg tgggttcgat tatcgtcaat aacaaattga
     1021 gccagtgagc gaaggctggc gggttctttc ttcagtttga gttttttgcc ttcgagtgcc
     1081 gctgtatcca gcagac
//
LOCUS       DAOQDO010002467         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_182839_length_1092_cov_2.853423, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002467 DAOQDO010000000
VERSION     DAOQDO010002467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182839_length_1092_cov_2.853423"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..581)
                     /locus_tag="PKZ53_28030"
     CDS             complement(60..581)
                     /locus_tag="PKZ53_28030"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNJ44363.1"
                     /translation="MNFPVYLYRAGLTPEVEAIRTFFHNQAIRFTEIDVREDKNAQAN
                     VERWTEGRSDVLPLVRVGEKILALFLQPAPDVLSRMFERGSLIEDSEIDRLPITVFSA
                     GWCPDCRFLESYLQRAGKTFAKVDIESTPGAPEKIIQWSGGRRVIPTVRVGEKIIFFN
                     PGTQIFGNFLAVR"
     gene            808..>1092
                     /locus_tag="PKZ53_28035"
     CDS             808..>1092
                     /locus_tag="PKZ53_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ44364.1"
                     /translation="MTQPKVQINKQIPDKLFFKIGEVCELVEVEPHVLRYWEQEFPQL
                     TPQKNQAGQRVYRRRDVEIAMRIKALREEEGFTIAGAKKRLAQEMRSGSKL"
BASE COUNT          306 a          297 c          209 g          280 t
ORIGIN      
        1 cattcttcat tcttcattct tcattcttca ttcttcattc ttcattcttc attcgccgat
       61 tatctcaccg ccagaaaatt cccaaaaatc tgagttccag gattaaagaa gataattttc
      121 tctcccaccc gaaccgtcgg aatcacccgc cgtccaccac tccactgaat gattttttcc
      181 ggtgcgcctg gtgttgattc aatatccacc ttggcaaacg tttttccggc tcgttgcaaa
      241 taactttcca aaaatcggca gtctggacac cacccggcgc taaaaactgt aatcggtagc
      301 cggtcaattt cgctgtcttc aatcagactt ccccgctcaa acatccgcga caacacatca
      361 ggtgctggct gcagaaacag tgccaggatt ttttcaccga cccgaaccag tggcaaaaca
      421 tcgcttcgac cttcagtcca gcgctcaaca ttggcctgcg cgtttttgtc ttctcgaaca
      481 tcaatttcag taaatcggat ggcctggttg tgaaaaaaag ttcgtatcgc ttcaacttcc
      541 ggtgtcagcc cggcacgata cagatagaca ggaaaattca taagcactcc ttaacaaact
      601 atatatcttc aataagaatt tcttactata cccttatcaa aactgggcaa acagccatct
      661 ttgctgaaag acagaggaag atcaatccca agtccccatt gacatttttt atcactctcc
      721 gtaacatcct gtcagagcta gccttatgtc gtacaagggg gtcacctctc atccctcatc
      781 acattcacca tctaacacag gtgcgacatg acgcaaccca aagtgcaaat caataaacag
      841 attccggata aactgttctt caaaatcggc gaggtgtgcg aactggtcga agtcgaacca
      901 cacgtgctgc gctattggga acaggagttt ccgcaactta caccacaaaa aaatcaggct
      961 ggacaacggg tctatcggcg gcgcgatgtt gaaatcgcga tgcggattaa agccttgcgt
     1021 gaagaagaag gctttaccat tgctggcgcc aaaaaacgtc tggcgcaaga aatgcgatct
     1081 ggttcaaaac tg
//
LOCUS       DAOQDO010002468         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_183155_length_1091_cov_2.900579, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002468 DAOQDO010000000
VERSION     DAOQDO010002468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183155_length_1091_cov_2.900579"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKZ53_28040"
     CDS             complement(<1..693)
                     /locus_tag="PKZ53_28040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNJ44365.1"
                     /translation="MPNLQGVRRRIKAVRSMRQITKAMKLVSTSRLKRAQDRVTAARP
                     YSRKMTEVLENLASRATGFSSPLLEQRTGSRVLLVLVTGDKGLCGAFNTNLIRAANLF
                     IRENPDAQIEMVAIGRKGRDFFRRRNIKIRKEYIGITAKAVTYEIAAEIAHEVVEMFS
                     GSSTETLEESAEKTEFETPDKVVLIYNEFKSVIQQVVRTEQLLPIGRFSEQTEDGQGG
                     SDSSTDYLYEQPP"
     gene            complement(848..>1091)
                     /locus_tag="PKZ53_28045"
     CDS             complement(848..>1091)
                     /locus_tag="PKZ53_28045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100113.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNJ44366.1"
                     /translation="VEKQVFLVWAATGGHVDDIPVAELRRFETELMSFLDNSKSSLLK
                     KILDKKELTGEIKDEMKAAIDEFKKTFTVRGQAAKA"
BASE COUNT          233 a          297 c          268 g          293 t
ORIGIN      
        1 tggtggctgc tcatagagat agtcggtgct tgaatccgaa cctccttgcc catcctccgt
       61 ctgttcacta aagcgtccaa tggggaggag ttgttcggtc cgaaccacct gctgaatcac
      121 ggatttgaac tcgttataaa tcagaacgac tttatctgga gtttcaaact cagttttttc
      181 cgccgattct tccagtgttt cagttgaact accgctaaac atctccacca cttcgtgggc
      241 aatctcggcg gcaatctcat aggtgaccgc tttggccgtg atgccaatat attccttgcg
      301 aattttgatg ttgcgtcgcc ggaagaaatc ccggcctttg cgtccgatgg ccaccatttc
      361 gatttgagca tccggatttt cacggatgaa aaggttggcc gcccggatca ggttggtgtt
      421 aaacgccccg cataaccctt tatcgccagt caccaggacc agcaagaccc ggcttccggt
      481 gcgctgttct aacagtggac ttgaaaaccc ggttgcccgt gaggccagat tttcaagcac
      541 ttcggtcatt ttcctggagt atgggcgcgc tgcggttact cgatcctgcg cacgtttcaa
      601 gcgcgaggta gacaccagtt tcatcgcttt ggtgatctgg cgcatgctgc gcaccgcctt
      661 aattcgtcgc ctgacaccct gtaaatttgg catagcttta ttgtcggaaa ttcttgaggg
      721 tttaggtttg aaggttgggg attagggaaa cggttcctgt atcttcaggg agtgatgagg
      781 ccgaggaata tcgaaaaccc acatgggttt caaccccaag ccccaagccc taagccccaa
      841 gccctaacta ggcttttgct gcctggccgc gaacggtgaa ggtcttcttg aactcgtcaa
      901 tcgctgcctt catttcatcc ttgatttcgc cggtcaattc cttcttgtcc agaatctttt
      961 tgagcagtga cgacttgctg ttatccagga aggacatcaa ctcggtttca aaccgacgga
     1021 gttcagccac cggaatgtca tccacgtgac cgcccgtggc agcccacacc aggaagacct
     1081 gcttttcaac t
//
LOCUS       DAOQDO010002469         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_183504_length_1090_cov_2.914976, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002469 DAOQDO010000000
VERSION     DAOQDO010002469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183504_length_1090_cov_2.914976"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1090
                     /locus_tag="PKZ53_28050"
     CDS             109..>1090
                     /locus_tag="PKZ53_28050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44367.1"
                     /translation="MRRKPQLHWKYRQQGVALAIALILLGVLMVFTALAATLMTSETR
                     LSAAQYDNARTFNAGMASLELMTDRINTLFTSNFNPTAAEIAEASGSVPIFKDGSNDL
                     FTFEQKVTQLGGGRALVTVLDPPFEGLTAKERLFRLETIAQDNQSQTQVRLIRDVANY
                     QIPIFQFAVFGFDSLDVSPGPRLWLGGRTHTNRNLFLMVRQGGNRTEFYGKITTVGEV
                     CTEILPSGRGVVSGESEPGRHRIYDKGTLSLRDIEPSVTNATGPDNPGVAASYDYNLV
                     DDPSSPNGGIKTSGAQPSGLVVTGIPRLLLPTESKGFQAIEILRRAVPADE"
BASE COUNT          262 a          278 c          294 g          256 t
ORIGIN      
        1 cggcaaactc ttaggaaata gttgaaaaac aaaggtttgt gacctgtttt agggagaaaa
       61 cgaattatga agtacacacg gttgagtcac acgcgacacc gagcgccagt gcgacggaag
      121 ccgcagcttc actggaaata ccgtcagcag ggtgttgccc tggcaatcgc cttgattttg
      181 ctcggcgtac tgatggtatt taccgcattg gctgcgacat taatgacttc agaaacccga
      241 ttaagcgcag cacagtatga caacgcccgg acgttcaatg ctggtatggc cagtctggag
      301 ttgatgaccg acagaatcaa caccctgttt acatcaaact tcaacccgac ggcggctgaa
      361 attgctgagg catctggctc ggtaccgatt tttaaagatg ggtcaaacga cctgtttacc
      421 tttgaacaaa aggtaaccca gttgggaggt ggtcgagccc tggtcacggt gcttgatccc
      481 ccgtttgaag gactgactgc caaggagcga ttgttccgcc tggaaacaat tgcccaggat
      541 aatcagagcc agacccaggt ccgcttgatt cgtgatgtgg ccaactatca gattccgatc
      601 ttccagtttg ccgtctttgg atttgacagt cttgatgtct ctcctggccc gcgcttgtgg
      661 cttggaggcc gaacgcatac caaccggaat ctcttcctca tggtgcgtca gggtggaaac
      721 cgcaccgagt tctacggaaa gatcacgact gtcggcgaag tttgcaccga aatcctgccc
      781 agtgggcgtg gtgtcgtgtc gggtgaaagt gaacctggac ggcatcggat ttatgacaaa
      841 ggaacgttgt ccctccgtga tattgaaccc agtgtcacca atgccaccgg gcctgataac
      901 ccaggcgtgg ctgccagtta tgattacaac ctggtagatg acccgagttc ccctaacggt
      961 ggtatcaaaa cttcaggtgc ccagccatcg ggactggtgg tgaccggaat tccgcgtctt
     1021 ttgctgccga ctgaaagcaa aggcttccag gcgattgaaa ttttacgtcg tgccgttcca
     1081 gccgatgaac
//
LOCUS       DAOQDO010002470         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_183531_length_1090_cov_2.590338, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002470 DAOQDO010000000
VERSION     DAOQDO010002470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183531_length_1090_cov_2.590338"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKZ53_28055"
     CDS             <1..832
                     /locus_tag="PKZ53_28055"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44368.1"
                     /translation="ESIRPTLIDYRGDGWLFSTPRGINFFSELFERGNPQNPDRNPQY
                     RSFQLPSWCNPHLPKAEIREMQFDMTQRGFEQEVEAKILPDGNGVFRYYTGPGVTHCA
                     RQERAIDGHTYVFGLDWGRSKDFTVISVFDETLKACVNLDRFNQVGYTVQRNRIKGLY
                     ERFKPHTILAESNSIGEVNIENLKRDGLPVRPFFTSNASKANAVEALQLAFERGDIHI
                     IKDPILIGELGKYEEQRLSGGLVKYGAPDGYHDDCVTALFIGWHACSIKKTRLSDFEI
                     "
     gene            906..>1090
                     /locus_tag="PKZ53_28060"
     CDS             906..>1090
                     /locus_tag="PKZ53_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44369.1"
                     /translation="MDTTANSPDYRSQQYQKMHEDLVKVHDAWGGTKTIRAAGTTYLP
                     KWPKEKPLAYQRRLTKS"
BASE COUNT          293 a          276 c          271 g          250 t
ORIGIN      
        1 ggagagcatc cggccaaccc tcattgacta caggggcgac ggctggctgt tctcgacacc
       61 acgcggaatc aatttctttt cagaattgtt cgaacgagga aatcctcaaa acccagatcg
      121 aaaccctcaa taccgatcct tccagcttcc ttcctggtgc aatcctcacc tccccaaagc
      181 tgaaatcaga gaaatgcagt ttgatatgac ccagcgaggc ttcgaacagg aagttgaagc
      241 caaaattctg cctgacggga atggtgtttt ccgatactac accggccctg gtgtgaccca
      301 ttgtgcccga caggaacggg caattgatgg ccatacctat gtgttcgggc ttgactgggg
      361 acgctcgaaa gacttcaccg tgatttcggt ttttgacgaa acactcaaag cctgtgtcaa
      421 cctggatcgc ttcaaccagg tgggctacac cgttcagcga aatcggatca agggacttta
      481 cgaacgattc aagccccaca ctatcctcgc cgaatcaaac tcaatcgggg aagtcaatat
      541 tgaaaatctg aagcgagatg ggcttccagt tcgtcccttt ttcaccagca atgccagtaa
      601 agccaatgct gtcgaggcgt tgcagttggc tttcgagcgc ggcgacattc acatcatcaa
      661 ggatccaatt ctgattggtg agcttggaaa gtatgaggag caacggctgt caggcggcct
      721 ggttaaatat ggtgctccgg acggttacca tgacgattgt gtgaccgcac tttttattgg
      781 ctggcacgca tgctcgatca aaaagaccag gttgagtgat tttgagatct aaggctgttt
      841 ggtcaccaaa atcagtcttt caagaatgaa taggattccc ctgaatctaa accttggaga
      901 ctcaaatgga taccacagca aacagtccgg attatcgctc gcaacagtac caaaagatgc
      961 acgaggacct ggtgaaggtt catgatgcct ggggtggcac caagacaatt cgggcggcgg
     1021 gaacaacgta tcttcccaaa tggccgaaag agaagccgtt ggcctaccaa cgacggttga
     1081 caaaatcctt
//
LOCUS       DAOQDO010002471         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_183650_length_1090_cov_1.889855, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002471 DAOQDO010000000
VERSION     DAOQDO010002471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183650_length_1090_cov_1.889855"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKZ53_28065"
     CDS             <1..356
                     /locus_tag="PKZ53_28065"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HNJ44370.1"
                     /translation="VLVAAGLYFLWRHNSASPLRTSTPTLPPQPIEQPIKGKGISIQQ
                     PIIHEPGIVFTLQAGSDLIGKTCPNEGTNLGRQWTFKPGDVVVQCPRCGTPHHLSCWK
                     HNQCQCWVHHCKAAA"
     gene            509..>1090
                     /locus_tag="PKZ53_28070"
     CDS             509..>1090
                     /locus_tag="PKZ53_28070"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNJ44371.1"
                     /translation="MNREQVFEITLRKLLEPIGAFLADDSISEIMINGPKEIFIERGG
                     QLERTPCAFGDEDSLLRAARNIAQYSGKRLTAETARLDGRLPDGSRVHMVFAKNSRKG
                     LCLSIRKFSRRAFSLTGLAELGVLSPEAREYLELIIALGRNLIVSGGTGTGKTSLLNA
                     ISAAIPTRERIVVIEDTTELKLQQPHVLSFEATP"
BASE COUNT          280 a          289 c          265 g          256 t
ORIGIN      
        1 tggttctggt cgcggcagga ctctatttcc tgtggagaca caactcagca tcgccacttc
       61 gtacctcaac gcccacactt ccccctcaac ccatcgaaca accgatcaaa gggaaaggaa
      121 tttctattca gcagccgatc atccacgaac cagggattgt gtttaccctc caggctggtt
      181 ctgatctgat cgggaagact tgccccaacg aaggaaccaa tctcggaagg cagtggacgt
      241 ttaaacctgg cgatgtcgtg gtccaatgcc ccaggtgcgg gacgccacac cacctctcct
      301 gctggaaaca taaccagtgc cagtgttggg tgcatcattg caaagcggcg gcttgaaccg
      361 tgagctacga ggcatcgccc cagaccactc aagcagcttt taatatcgtt tgggaatgga
      421 gtcctcggat tacaagaagg ctaaactgtt gaaagaattg tatttccctg aaccctgaac
      481 cctgaaccct aattggtatg acatcattat gaatcgagaa caggttttcg aaattaccct
      541 gcgtaaactc cttgaaccaa tcggagcatt tctggccgat gattccattt ctgaaattat
      601 gatcaacggt ccgaaggaaa tctttattga gcgcggaggc caattggaac ggaccccctg
      661 tgcctttgga gatgaagatt cgctgttgcg ggcagcacga aatatcgccc aatattcggg
      721 aaaacgactc acggcagaaa cagcccgctt agatggacgc cttccagatg gttcacgagt
      781 ccatatggtg tttgccaaaa actcacggaa gggtctgtgc ctctcgattc gcaaattttc
      841 acggcgggca ttttcactga ctggattggc tgaattgggg gtgctttcgc cagaagccag
      901 agaatatctt gaactcatca tcgcgttggg ccggaacctc atcgtttccg gcggaactgg
      961 aactggaaag acgtctttgc tcaacgcgat ttcagccgcg attccaacca gggaacgaat
     1021 tgtcgttatt gaagacacca ccgaattaaa gctccagcag cctcatgtcc tgtcgtttga
     1081 agcaacacct
//
LOCUS       DAOQDO010002472         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_183836_length_1089_cov_3.084139, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002472 DAOQDO010000000
VERSION     DAOQDO010002472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183836_length_1089_cov_3.084139"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>1089)
                     /locus_tag="PKZ53_28075"
     CDS             complement(147..>1089)
                     /locus_tag="PKZ53_28075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ44372.1"
                     /translation="MPLIEFVLPPGLTPEVDTSFEVESTLRLGSPPPPPNFVATSAPL
                     PSLEEFTEPAVEDDDEEPRDRSRMPPTPADLEAAEGMDFETHYDLGISYKEMGLYEQS
                     VRELHRAIQKVSPTDEAGRYMSCCTLLGMCYREQQMYLQSVDWFQHALTLTKPADASY
                     LALQYELGLTYEMAGQTFNALAAFSEVYALDVHFREVIEKVRFLQNEVIVRAERMQKM
                     IPIHIRAIDARGQRMDEDTWIVNISRRGAGLKTNIEHESGTLIEVEFTQAGKRHLGKV
                     VWCAPSKDQDSSYRMGITLITDAELGVEDWPRPIAQG"
BASE COUNT          245 a          295 c          261 g          288 t
ORIGIN      
        1 ctgctgttta ctttttctgg attggtcagc cccgcttcga ggcacacgtt tgccgttccc
       61 cgagcgcggg ctggccgtcg cctgagtgtc agacgtttgc gcatcgagtt gttccaaatg
      121 actgtcacca ttcattttgg attgaatcat ccctgtgcta ttggtcgagg ccaatcctca
      181 acgccaagtt ctgcgtcagt gatcaaggta attcccatgc gataggaact atcctggtct
      241 tttgacggag cgcaccatac aacttttcca aggtgtcgct ttccagcctg agtaaattca
      301 acttcgatta aggttccgct ttcgtgctcg atatttgttt ttaatccggc cccgcgccga
      361 ctgatattta caatccaggt atcttcgtcc atccgctgtc ccctggcgtc aatcgcccga
      421 atatgaatcg ggatcatttt ttgcatccgc tcagcccgca caatgacttc gttttgcagg
      481 aatcgaactt tttcgatcac ttcgcggaaa tgcacatcca gggcatacac ctcagaaaaa
      541 gccgccaggg cattaaacgt ctggccagcc atttcatagg tgagcccaag ttcatattgc
      601 agagccagat aactggcatc agccggtttt gtcagggtga gggcatgttg aaaccaatca
      661 accgactgga gatacatttg ttgttcgcga tagcacatcc ccagcaatgt gcaacaactc
      721 atatatcggc cagcttcatc cgtgggggat actttttgaa ttgcccggtg aagctcccgg
      781 acagattgct catacaagcc catttcttta tatgaaatcc ccagatcata gtgggtttca
      841 aagtccatcc cctcggcagc ttccaaatca gccggagtcg gtggcatacg tgaccggtca
      901 cgtggctctt cgtcatcgtc ttccaccgca ggctcggtaa actcttcaag cgatggcagt
      961 ggggctgatg tcgcgacaaa atttggcggc ggtggtggtg acccaagcct gagcgtagac
     1021 tcaacttcaa aggaagtatc aacctcaggt gtcaatcctg gcgggagcac aaattcgatc
     1081 agcggcatt
//
LOCUS       DAOQDO010002473         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_183839_length_1089_cov_2.977756, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002473 DAOQDO010000000
VERSION     DAOQDO010002473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183839_length_1089_cov_2.977756"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKZ53_28080"
     CDS             complement(<1..252)
                     /locus_tag="PKZ53_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380453.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNJ44373.1"
                     /translation="MSSKPELSTKETLSFQTEVKQVLNLITHFLYSNKEIFLRELLSN
                     ASDAADKIRFLALSDSTLLEGDSEFKIRVSFDKTNRTVTI"
     gene            563..862
                     /locus_tag="PKZ53_28085"
     CDS             563..862
                     /locus_tag="PKZ53_28085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44374.1"
                     /translation="MCIKSGNDFRVTAVHSNRCSTLRATLLEAQRHVNIGNSPLGDLF
                     RILDWPDALQTLSGLTQVSISAVAVLYQTSFAPNSYGFPHQFPVLSDGSTLSSQL"
BASE COUNT          273 a          263 c          270 g          283 t
ORIGIN      
        1 aatagtgacc gttcggttgg ttttgtcaaa ggaaacccgg attttgaatt cgctatcacc
       61 ttccaggagc gtgctgtcag acaatgccaa aaaacggatc ttatcggcgg catctgacgc
      121 gttggaaagc aattcacgca aaaagatttc tttgttgctg tacagaaagt gggtaatgag
      181 attgagaacc tgcttgactt ccgtctggaa actcaaggtt tctttggtcg aaagttccgg
      241 cttgctgctc atagtgtcta tctccttcaa gatgaagagt tatcaaaaag gataccgttt
      301 accacaaatt ctgtcagttg aaaacctcct cgtccgacaa aggtgaaaaa cgaatggttc
      361 caaccgtcct ctttccatcc ggcgaacctg aacgctagcc agtctggttc aatgagcact
      421 gcctcacaga tcggggaacc tgttctgcaa attgagactt tgtcaaactg agggccagca
      481 acgtcctgca attgcattga gcttgattga taggcaaacc agcatcgggt ttgaaacggg
      541 ggagttgctt ccataaagca atatgtgtat aaagtcaggc aatgactttc gggtcacggc
      601 ggttcactct aaccgttgca gcacgttaag ggcgactctg ctggaggccc agcgacacgt
      661 gaacattggg aattccccgt tgggcgatct gttccggatt ttggattggc cagatgctct
      721 ccaaaccctt tcagggctga cccaggtgag tatttcagcg gttgcggtgc tctaccagac
      781 ttcattcgct cccaattcat atggctttcc ccaccagttt cctgtgttga gcgatggctc
      841 aaccctttca agccagttgt gacaagaaca gtgcgatgat gagcccggaa aatggtggat
      901 gccgttcact caagtttgcg gctaaaatgt tgattccgaa tgagatggtt ggcatctttt
      961 cagccagggg attatttaaa gaaatctgtg cccggatcaa ctcatccagc ccagattgcg
     1021 caggtcagcc acaactgacg tcctatcaat tcatggcctg actggccgcc aggaggaatt
     1081 ccgtgacac
//
LOCUS       DAOQDO010002474         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_184153_length_1088_cov_3.553727, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002474 DAOQDO010000000
VERSION     DAOQDO010002474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184153_length_1088_cov_3.553727"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PKZ53_28090"
     CDS             complement(<1..1025)
                     /locus_tag="PKZ53_28090"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ44375.1"
                     /translation="MPHPYHLLVVEDENAQRRLIADILRKDGFQVQEAPSGIAALEIL
                     ENQPVDLVLCDWRMPGMSGGEVLAEVRKRQYPSSFIVMTAYGSIAHAVEAVRLGADDY
                     LAKPFEKEALLLAIQRVLRTKKLEQENLRLREEIVQRDQCGEIFGQAESMQRLYRTLK
                     KVAITDATILIQGESGTGKELVARTLHTESPRAKAPFVAVNCAAIPETLMESELFGFE
                     KGAFTGAQKRREGKFEEAEGGTLFLDEVPSMPLALQATLLRVLQERRFTRLGGRGEVS
                     CNVRIIAASNRDLPRLVSEGAFREDLYYRLNVVPLTIPPLRERREDIPLLVSVFTKQA
                     EARYNVTV"
BASE COUNT          265 a          279 c          298 g          246 t
ORIGIN      
        1 accgtgacgt tgtagcgggc ttcagcctgt ttggtaaaga ctgaaaccag aagaggtatg
       61 tcttcacgac gttcacggag cggcggaatg gtcaggggaa cgacattgag gcgataatag
      121 aggtcttcac ggaacgcgcc ttcactcacc agtcggggca ggtcccgatt tgaggcagca
      181 atgatccgga cattgcacga cacttcacca cgcccgccaa gtcgggtaaa tcggcgttcc
      241 tggagcaccc gcaggagggt cgcttgcaag gcaaggggca tagacggcac ttcatcgagg
      301 aaaagcgtgc cgccttcagc ttcttcaaat ttgccctcac ggcgtttttg ggcgccggta
      361 aaggcgcctt tttcaaaccc aaataactcg ctttccatca gtgtttcagg aatggcggca
      421 cagtttactg ccacaaatgg agctttggcg cgtgggcttt cggtgtggag cgtccgggcc
      481 accagttctt tgccggtgcc agattcacct tgaatcaata tcgtggcgtc agtgatggcc
      541 acttttttca gtgtccgata caggcgctgc atggattcag cctgaccaaa aatttcgcca
      601 cattgatcac gctgaacaat ttcctcacgg agccggaggt tttcctgttc gagctttttg
      661 gtgcgcagca cccgctgaat cgccagcaac aacgcctctt tttcaaaggg tttggccaga
      721 tagtcgtccg cacccagacg cacggcttca acggcatggg caattgaacc ataggccgtc
      781 atcacgataa atgagctggg atactgtcgt ttgcgaactt cagccagtac ctcgccaccg
      841 ctcatgccgg gcatgcgcca gtcacaaagc acaagatcaa ctggttgatt ttcaaggatt
      901 tccagtgcgg caattcccga tggcgcttcc tgaacctgaa atccatcttt gcgcagaata
      961 tcggcgatca accgtcgctg ggcattttca tcttcgacaa caaggaggtg gtaagggtgg
     1021 ggcatgggcc taagagaatg aagaatgaag aatgaagaat gaagaatgaa gaatgaagaa
     1081 tgaagaat
//
LOCUS       DAOQDO010002475         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_184579_length_1087_cov_2.218023, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002475 DAOQDO010000000
VERSION     DAOQDO010002475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184579_length_1087_cov_2.218023"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKZ53_28095"
     CDS             complement(<1..1060)
                     /locus_tag="PKZ53_28095"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ44376.1"
                     /translation="MLPVVAQDSTSASLPPEAQFLEVGKPITAKIKGTERHAYRLRVP
                     QGQMCLVQVEQLGVDLVLEWFASDGTSLGLTNRRLSRGTEQAVFLAEQSPLDLKLEVR
                     PQLQPVNPHGYQIQIAELREATQYDTLTIAAQQAYRQASQLSSNKTGKPDYQKAIEKY
                     QSALNLYRLIPDPIGEGYALHHMARAYMRLNQRDKALEYFQQALNVWDANQNKLDRAL
                     TLTSLSFFSTNTGELAKSVTSATEAAEIYRQFQDQDGEAGALNNLASALIYLGALRRA
                     QECLNRVLVIDQANGSLFDQATTLNNLAATYDNVGETQRARVCYEQALELRRLAKDRQ
                     GEAFTLINIAANQGTLSNY"
BASE COUNT          244 a          265 c          299 g          279 t
ORIGIN      
        1 gataatttga tagggtccct tgattggcag caatattaat taaggtaaat gcttcgccct
       61 gacgatcttt ggcaagtcgg cgcagttcga gtgcctgttc atagcagacc cgggcacgct
      121 gggtttcccc aacattgtca taagttgcgg ccaggttatt gagcgtggtt gcctgatcaa
      181 aaagcgatcc attggcctga tcaatgacca ggactcgatt cagacactcc tgagcccgac
      241 ggagtgcgcc cagatagatc agggcacttg ccagattatt gagagcacca gcctcgccat
      301 cttgatcctg aaactggcga tagatttcag ccgcttctgt cgcggaggtg actgatttgg
      361 ccagttctcc tgtgttggta gagaaaaaac tcaaactggt tagggtgagt gcccggtcaa
      421 gtttattctg attggcatcc cacacgttga gggcttgctg gaagtattcg agggctttgt
      481 cccgctgatt gagtcgcata tacgcccgag ccatgtgatg gagcgcatat ccttccccga
      541 ttggatcagg aatcaaccgg tacaaattca gcgcggactg gtatttttca attgcctttt
      601 gataatccgg tttaccagtt ttattgctac tcaactgact ggcttgccga taggcttgct
      661 gggcagctat ggtcagtgtg tcatactggg tcgcttcgcg cagttcagcg atttgaatct
      721 gatacccgtg tggattaaca ggttgaagtt gtgggcgaac ctccagtttc aggtcgagcg
      781 gggattgttc agccagaaag actgcctgtt ccgtgcctct gctgagacgt cggttggtca
      841 gtccaaggct ggtgccgtca ctggcaaacc actcaagcac caaatcaacc cccagttgct
      901 caacctggac cagacacatt tgaccctgtg gaacccgaag gcgataggca tggcgctcgg
      961 ttcctttgat tttggcagtg attggttttc caacctccag gaattgagcc tccggcggaa
     1021 gcgaagctga tgtggagtct tgggccacaa ccggtaacat cggaagcagt aaagacagac
     1081 tgaggag
//
LOCUS       DAOQDO010002476         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_184616_length_1087_cov_2.000000, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002476 DAOQDO010000000
VERSION     DAOQDO010002476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184616_length_1087_cov_2.000000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKZ53_28100"
     CDS             complement(<1..858)
                     /locus_tag="PKZ53_28100"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNJ44377.1"
                     /translation="MGLGSVDTPSNHGVSTPLPVEPASIALNKETDLVTFAGTVLGTP
                     MYMSPEQCQGIAIDHRSDLYSLGVILYRMLTGEPPFTGNFTFLMKQHIEDEPPNPCEK
                     NTGLSAAVAEVMLSALRKDPAQRPPSAQAFAVALRANAEGEEPLFKEAGDLYRTNFWS
                     FFPISLVIHAPYAVLNGLIFLIWLALSQPGGARSLRALQFFHSTSWIVTATIVFFAGV
                     VNFAAFQPVVNSLQSVPDRSVSIVSTIKNFFAILPKLATTAFFCAIAILRGLATGFRC
                     GIRTVTCTAL"
BASE COUNT          292 a          256 c          304 g          235 t
ORIGIN      
        1 cagcgccgta cacgtcacag ttcggatacc acatcggaac ccggtggcca gcccacggag
       61 aatggcaata gcacagaaaa aggccgtggt agccagtttt ggcaaaatag caaagaagtt
      121 tttgatcgtg gatacaattg atactgaacg atctggcacg gattgtaatg agttgaccac
      181 gggttggaag gccgcaaagt tgacgactcc ggcaaaaaag acaatggtgg ccgtcacaat
      241 ccaggaggtg gagtgaaaga attgtaaggc cctcagagat ctggcgccac ccggctgact
      301 caaggccaac catatcaaaa agatcagtcc attcaacact gcataagggg catgaatcac
      361 cagtgaaatc ggaaaaaatg accagaagtt ggtgcggtat agatcaccag cttctttaaa
      421 aaggggttct tcgccttcgg cgttggctcg taaggcaacg gcaaacgcct gagcgcttgg
      481 tgggcgctgg gctggatctt tgcgcaacgc cgagagcata acttccgcca cagcggcgct
      541 cagaccggta tttttttcac aagggttagg cggttcatct tcaatatgct gcttcatcaa
      601 gaaagtgaag ttaccagtaa acggtggttc accggtaagc attcgataca aaatgacccc
      661 caggctgtac aaatctgaac ggtggtcaat ggcaatcccc tgacactgtt ctggcgacat
      721 atacattggt gttcccaaca cggtcccagc aaaagtaacc agatccgtct ctttattgag
      781 tgctatcgaa gccggttcca ccggcaacgg tgtggaaacc ccatggttgg aaggtgtatc
      841 aactgaaccc agacccatcg tgtctgactc agggaatttc agggtcgttg gttcgtgaag
      901 ggaatcctgg tttgactgac tgagagctgg cccgtctgca gaacggccag gcgtaaatat
      961 tttgctcgaa aggtcaatcg tgcttcctga cggaacgggc cggggtggtg caggcgcaga
     1021 taaagagttc aatgtctgct ggggagacga cagattctgc ccagatggcg gatgtgagaa
     1081 tttcagg
//
LOCUS       DAOQDO010002477         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_184902_length_1086_cov_2.282250, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002477 DAOQDO010000000
VERSION     DAOQDO010002477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184902_length_1086_cov_2.282250"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKZ53_28105"
     CDS             complement(<1..419)
                     /locus_tag="PKZ53_28105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44378.1"
                     /translation="MDEFLNQFREEPRPEFAQDLYARLQTQKATTTSRRAWKWSPLLA
                     AASVAFVMGLALNVPTVRAIAQNFLDLFRVKKFAAVSIDIDRLKQLKDTNLDFRSLLT
                     SDASVPARPAPPQHVTSPEAAAQLAGIQVRTPKLDQFG"
     gene            complement(574..>1086)
                     /locus_tag="PKZ53_28110"
     CDS             complement(574..>1086)
                     /locus_tag="PKZ53_28110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ44379.1"
                     /translation="MGWMIPFAAKAQETDWEALYKEELPRIFNFFRYRVGDASLAEDL
                     TSTTFEKAWQHRDRYRRDLAGFSTWLFTIARNVASDHFRRHTVQVPLEDIHDVPSGSN
                     PEASAVKQAEFQRLSALLASLPERERELLALKYGAELTNRSISHITGLSESNVGSILH
                     RTIHSLRERW"
BASE COUNT          265 a          280 c          277 g          264 t
ORIGIN      
        1 ccaaattgat ccagctttgg cgtccgcacc tggatcccgg ccagttgggc ggcagcttca
       61 ggtgaggtga catgttgtgg tggggcaggt ctggctggaa cactcgcatc actggtcagt
      121 aaagaccgaa agtcaagatt ggtgtctttg agttgtttca accgatcaat atctatcgaa
      181 actgccgcaa atttcttgac tcgaaacaaa tcgagaaagt tttgggcaat cgcccgcacc
      241 gtgggcacgt tcaacgccag acccatcaca aaagcaacgc tggcggctgc cagcaatggc
      301 gaccatttcc aggctcgacg gctcgtagtt gtcgcttttt gggtttgaag ccgggcatat
      361 aaatcttgtg caaattcagg ccggggttct tcacgaaact ggtttagaaa ctcatccata
      421 ctggttttcc tcattatttg ggaatcagca acctcaggtt gcgacctttt tctttttcac
      481 ggtttccagc cacaaccagc agtgaccgtt tttgctggtt gtggtccggg tcttctcaca
      541 aacgacacag tagacagagc ttgaagggca agctcaccaa cgctcacgca gcgaatgaat
      601 ggtgcggtgc aaaatcgacc cgacgttgga ctcgctcaat ccggtaatgt gagagattga
      661 acgattggtt aactccgcac catatttcag tgccagcaac tcgcgctccc gctcaggaag
      721 gcttgccagc aaagctgata acctttgaaa ttcggcctgt ttgacagccg aagcctcagg
      781 gtttgagcct gacggcacat cgtgaatgtc ttcgagtggc acctgcacgg tgtggcgtct
      841 aaagtggtca gacgccacat tgcgggcaat cgtaaacagc caggttgaaa atccagccag
      901 atcccgccga tagcgatcac gatgttgcca ggctttttca aaggtcgttg aggtcaaatc
      961 ttcagccagc gaagcatccc caactcgata gcgaaaaaag ttaaaaatcc gtggaagctc
     1021 ttctttataa agggcttccc agtcagtttc ctgggctttg gctgcaaagg gaatcatcca
     1081 tcccat
//
LOCUS       DAOQDO010002478         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_185305_length_1085_cov_1.751456, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002478 DAOQDO010000000
VERSION     DAOQDO010002478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185305_length_1085_cov_1.751456"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..824)
                     /locus_tag="PKZ53_28115"
     CDS             complement(279..824)
                     /locus_tag="PKZ53_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNJ44380.1"
                     /translation="MKKTLMMLALAIVAVVFSDSALAQCQQGKSTARKASYSRTTHNS
                     TPDIVDTAISAGSFMTLVTAVKAAGLVETLKGEGPFTVFAPTDEAFAKIPKATLQSLL
                     RPENRDQLTKILTYHVLPGRVTAAQVARLHDAQTVQGGSVHFKANGNGIKVNDSKVVK
                     ADIETSNGIIHVIDTVLMPGE"
BASE COUNT          275 a          248 c          271 g          291 t
ORIGIN      
        1 tataagaatg aagaatgagt ggtgaatgat gagccagggg atacaaattt gggatcaggt
       61 ttggcgagaa accgattttt tcaatcttgt taccctggct aacgatctgc attccaaaga
      121 gatacatgtt tcatcattta ccattcatcg tatgccatct tggaatgaag cggtcgggtg
      181 agaaaacagt taagttattc attgaaataa gcttagtgag ctactgacga ctgacgactg
      241 acgcaaactg gtattagatt tgggggctat catcccaact attcacccgg catcagcacc
      301 gtgtcaataa catggatgat cccattactg gtttcaatgt ctgctttgac gaccttcgag
      361 tcattgacct tgatcccatt gccgttggct ttaaagtgaa ctgacccgcc ctgaacggtt
      421 tgggcatcgt gcaatcgtgc gacctgagcg gcagtaaccc gtcctggaag aacatgatag
      481 gtcaaaattt tggttaactg gtcacgattt tcaggtcgca acaacgactg caacgtggct
      541 ttgggaattt tcgcgaaggc ttcgtcggtt ggggcaaaaa cggtaaaggg gccttcaccc
      601 ttgagggtct cgaccaggcc agccgctttg acagcggtga ccagggtcat aaaactcccg
      661 gcactgattg cggtatccac aatgtcgggt gtgctgttgt gggttgtgcg ggaatacgaa
      721 gctttccgag cggttgattt cccctgctgg cactgtgcca gcgctgaatc tgaaaacacc
      781 acggccacga tggccagagc caacatcatc aaagtctttt tcattttttg tctccgtgca
      841 gttgaattta gaactttttt ctgtggaagc accctgtgtg gaagattgtg ggcgctccac
      901 tggcagtttt cgtggctttt ccaaatgtgg attcaaaagt ccggaaattt gaattccagc
      961 tctcaggaat cagtgaactg aaaaacacca gcttcggtca gcccgcctgg aactcgccgt
     1021 gtcagaaaaa tcagattggc ataagacagt gcatcaccaa tcaacctgat atagagttct
     1081 tctcc
//
LOCUS       DAOQDO010002479         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_185785_length_1083_cov_3.809339, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002479 DAOQDO010000000
VERSION     DAOQDO010002479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185785_length_1083_cov_3.809339"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKZ53_28120"
     CDS             <1..478
                     /locus_tag="PKZ53_28120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HNJ44381.1"
                     /translation="GQTIASNVTSQEAKDFKVKIKLNPSPEVRDRLRPGMSATAVITT
                     TTRQNVVAIPLQALVSRDKNDDGKGTTPAPEADKPAGPVKVKREEIPGVYIDDNGKAK
                     FVAVKTGITGETDIEITEGLKEGQRIVIGPNRQLRTLKDGTQIVKEETKPENTEKK"
     gene            568..>1083
                     /locus_tag="PKZ53_28125"
     CDS             568..>1083
                     /locus_tag="PKZ53_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ44382.1"
                     /translation="MEATDTQHNNHTPLTPAPDTIIQTVDLWKTYRMGDETIHALHGV
                     SFEIKRNEYIAIVGPSGSGKSTLMNLIGCLDTPSKGEYWLNGKLVSQMDDDELAHIRN
                     KEIGFVFQTFNLLPRATALHNVELPLIYNGTPAAARTARAKEALEQVDLGHRMHHKPN
                     ELSGGQRQRVAI"
BASE COUNT          324 a          281 c          251 g          227 t
ORIGIN      
        1 cggtcagacc atcgcttcca acgtcacctc acaggaagcc aaagatttca aagtcaaaat
       61 caaattgaac ccatcgcctg aagtgcgtga tcgcctccgg cctggtatgt cagccacggc
      121 ggtcattacc accacaacgc gccaaaatgt tgttgccatt cctttgcagg cacttgtcag
      181 ccgtgacaaa aatgatgatg gcaaaggaac aacgccagcc ccggaagccg ataaaccagc
      241 cggccccgtc aaagtcaaac gtgaagaaat ccctggggtc tatattgatg acaatggaaa
      301 agccaagttt gtggctgtca aaaccggaat tacgggtgag actgacattg aaatcaccga
      361 aggtttaaaa gaaggtcagc gcattgttat tggaccaaac cgccagctcc ggaccttgaa
      421 agatgggacc cagatcgtca aggaagaaac caaaccggaa aataccgaga agaaataagc
      481 tgagagggta gacaagtctg tcttccctct ttaccatcat ttccacctga ctcctgatcc
      541 agaaaaacta tgtcaacaac aatgatgatg gaagccactg atacccaaca caacaaccat
      601 acgccactca caccagcccc agatacaatc atccaaacgg ttgatttgtg gaagacctat
      661 cggatgggtg acgaaacgat tcatgcctta catggcgtca gttttgaaat caaacgcaat
      721 gagtacattg ccattgttgg tccatcaggg tcaggaaaat caaccttgat gaacctgatt
      781 ggctgcctcg atacgccttc gaaaggtgaa tactggttaa acggaaagct tgtttcccaa
      841 atggatgacg acgaattggc tcacatccgc aataaggaaa tcgggtttgt gtttcagacg
      901 tttaacctgc tccctcgggc cacagcgttg cataacgttg aattaccatt gatttataat
      961 ggtacgccag ccgcggcgcg aaccgcccgc gccaaagaag cccttgaaca ggttgatctt
     1021 ggccaccgca tgcaccataa gccaaatgaa ttgtcgggcg gccagcgcca gcgtgtggcg
     1081 att
//
LOCUS       DAOQDO010002480         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_185898_length_1083_cov_2.178988, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002480 DAOQDO010000000
VERSION     DAOQDO010002480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185898_length_1083_cov_2.178988"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..536)
                     /locus_tag="PKZ53_28130"
     CDS             complement(57..536)
                     /locus_tag="PKZ53_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNJ44383.1"
                     /translation="MEEPQSIEKLVVENIQELKKYVGQDIALTSYYQIDQSRVDQFAT
                     LTEDRQWIHTDVERARRESPHGGTIAHGFLTLSLLNHFLLQAVEVKNVRLGLNYGLNS
                     VRFPAIVPTGAKIRAQVRLLSVEDVTGGIQAVWKFTITCQGNEKPSCVAEWVVRYYE"
     gene            complement(605..>1083)
                     /locus_tag="PKZ53_28135"
     CDS             complement(605..>1083)
                     /locus_tag="PKZ53_28135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44384.1"
                     /translation="LMIHTLIPLLQSIAAVIVNTDARKLPPLTIWHHLGTGFLILWAL
                     AVYPLLKVVGAAKRDPLAMVNPFVLVGAWSSLKSWILPPFGLMVIEFAALVVIGLILH
                     SLPGGVILMSALLAYASLTASYTFGVAVHKGWETLDLYYQEPEPDLFKQTNNKSKK"
BASE COUNT          289 a          250 c          287 g          257 t
ORIGIN      
        1 gttttgacga accaaaataa tttgggtaat gacaagaccc ggaacccgga acccgactat
       61 tcatagtacc tgaccaccca ctcggctacg caggacggtt tttcatttcc ctgacaggta
      121 atggtaaatt tccaaacggc ctgaatacca ccagttacat cctcaaccga aagtaagcgg
      181 acctgagccc ggattttggc accagtgggg acaattgccg ggaacctgac cgaattcaat
      241 ccataattaa gcccgagtcg gacatttttg acttccacgg cctgcaacag aaagtggttg
      301 agcagtgaaa gcgtcaggaa gccgtgagca atggtgccac cgtgaggtga ttcccggcgg
      361 gcacgctcca catcggtgtg aatccactgg cggtcttcgg taagggtagc aaactgatca
      421 acacgggatt gatcaatttg ataatagctg gtgagggcaa tgtcctgacc aacatatttt
      481 ttcaattcct gaatgttttc aacgacaagt ttttcaattg attggggttc ttccataggt
      541 attgagtcac gggattggtt cagagtacgg attttgctcg aaatacggct gctctgctga
      601 ggacttactt cttgcttttg ttgttggtct gcttaaaaag gtccggttct ggttcttgat
      661 aatagagatc gagggtttcc cagcctttgt ggaccgcgac tccaaaggta taggaggcgg
      721 tcagtgaagc ataggccagt aaggctgaca tcagaatcac gcctccggga aggctatgaa
      781 ggatcaggcc aatcacaacc agagctgcaa attcaatcac catcaggcca aatggcggga
      841 ggatccagga cttgaggctt gaccaggcac caaccagaac aaacgggttg accatggcaa
      901 gtgggtcgcg cttcgcggca ccaacaactt tgagcagtgg atagacagcc aaagcccaca
      961 atatcaggaa tccagttcca aggtgatgcc agatcgtcag tggcgggagt tttcgagcat
     1021 ccgtgtttac gatgacggct gctattgact gtaaaagtgg aattaaggtg tgaatcatca
     1081 ggg
//
LOCUS       DAOQDO010002481         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_186325_length_1082_cov_1.735151, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002481 DAOQDO010000000
VERSION     DAOQDO010002481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186325_length_1082_cov_1.735151"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKZ53_28140"
     CDS             <1..763
                     /locus_tag="PKZ53_28140"
                     /inference="COORDINATES: protein motif:HMM:NF021162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease"
                     /protein_id="HNJ44385.1"
                     /translation="AAMQLFGRVQDRLSHVLVSEDFETHPDFYYLPSQPKELEITDRF
                     GTVIKRVSTQDARIYLADIPFIRLRGLLANWQPEIVKYSDLVARAQEDLDLVESTYEI
                     HIWLCTGRRGWTVSIANRSARLTEREVFVYALLIEAGARHLGPIAVEEITESLIDATL
                     NRLFTTRGVPYISTEPLPTRLEFVNGLRAELKSWKAGDSYQLSLKESFIQTISKIHRK
                     FEEARLPPVCFIATRGERGALRYELKLAPERIVFG"
     gene            880..>1082
                     /locus_tag="PKZ53_28145"
     CDS             880..>1082
                     /locus_tag="PKZ53_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HNJ44386.1"
                     /translation="MKHVFVVSIGPVQDFIASARRSRDLWFGSWMLSELAKAAALKII
                     ELNGNRDGCLIFPSPNTINELKPG"
BASE COUNT          247 a          280 c          284 g          271 t
ORIGIN      
        1 agcggccatg caattgttcg gtcgggttca ggaccgactt tcccacgttc tggtcagcga
       61 ggatttcgaa acgcatccgg atttttacta tcttccgtct cagcccaaag aactggaaat
      121 caccgaccga tttgggacgg tcatcaaacg ggtttcaacc caggatgccc gtatctatct
      181 ggcggatatc ccctttatcc ggttgcgtgg gctgctcgcc aactggcaac cggagattgt
      241 caaatacagc gacctggtgg ctcgggccca ggaagacctc gacctggttg aatcaactta
      301 tgaaatccac atctggttgt gtactggtcg ccgtggctgg accgtgagta ttgccaaccg
      361 aagcgcccgc ctgaccgaac gcgaagtgtt tgtatatgca cttctgatcg aagccggcgc
      421 ccgacacctg gggccgattg cggtcgagga aattaccgaa tcgttgattg atgccaccct
      481 gaaccggctc tttacaaccc gaggggtacc gtatatctcc accgagccgc tgccgacgcg
      541 gctcgaattt gtcaacggat tgcgggcgga actcaaatcc tggaaagccg gtgacagcta
      601 tcaactgagc ctgaaagagt catttattca aacgatttcc aaaattcacc gcaagtttga
      661 agaagcgaga ctcccgccag tctgttttat tgcgacgcgg ggtgaacgcg gcgcgcttcg
      721 ctatgaatta aagctcgccc ccgaacgaat cgtgtttggt taacccatac cagtttgtag
      781 tcagtagtca gtagtcagta gttcgctaag cctatttcat tgaattacct ggcttttttc
      841 tcatgcaagg cttcccaaaa aaggaggtag gtgctgttta tgaaacatgt ctttgtggtc
      901 agtattggcc cggtccagga ttttattgct tcggcccggc gcagtcgtga tttgtggttt
      961 ggttcgtgga tgttgagcga actggccaag gctgcggcct taaaaatcat tgaattgaat
     1021 gggaatcgag acggctgcct gatttttccc agtccgaaca ccatcaacga actcaaaccc
     1081 gg
//
LOCUS       DAOQDO010002482         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_186772_length_1081_cov_1.469786, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002482 DAOQDO010000000
VERSION     DAOQDO010002482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186772_length_1081_cov_1.469786"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..167
                     /locus_tag="PKZ53_28150"
     CDS             27..167
                     /locus_tag="PKZ53_28150"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44387.1"
                     /translation="MGRSSALRQVRLDFLKRPGRAHPFYWASFILSGGWEPLKRGKAS
                     RE"
     gene            297..455
                     /locus_tag="PKZ53_28155"
     CDS             297..455
                     /locus_tag="PKZ53_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44388.1"
                     /translation="MSEINNEQELLQVTSGIKAGDSSGGVLSQTADPGEDAEITIGGQ
                     SMSDIRTP"
     gene            635..>1081
                     /locus_tag="PKZ53_28160"
     CDS             635..>1081
                     /locus_tag="PKZ53_28160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44389.1"
                     /translation="MLTIQKLVAFSLLLFLRSGGVVSGIQPAPSSPQFSNQLALQAPV
                     LVPGKIINQELKGGEVNRFQIRLKARQFLDLSVEQQGIDVVVRLLGPDGKRVTEVDSP
                     NGPDGPEPVVLIVRQSGTYTLEIKAPDPAAEPGKYQITCEPLRRATP"
BASE COUNT          282 a          254 c          274 g          271 t
ORIGIN      
        1 aatattactg acttctcagg cagggaatgg ggcggtcatc ggcactgcgc caggtacggc
       61 tggacttttt aaaacgccct gggcgagcgc atcctttcta ctgggcgagt tttatcttat
      121 ccggcgggtg ggaacccttg aagcgaggga aagcgagtcg cgaatagtca atcctggaag
      181 cacaaatgga tggctcgaag ggattgactt cagccggccc tattcagtag ggttgacgtt
      241 gtcagctaac acatccaaaa gataggtctc ctatctttaa tcacacaagg taatatatgt
      301 ctgaaatcaa caacgaacaa gaattgcttc aagtaacgtc tggaattaaa gctggtgata
      361 gttctggtgg ggtattgagc caaacagcag acccaggtga agatgccgaa atcaccatag
      421 gtggtcaatc aatgagtgac atcaggactc cataaaaaat aattgaattt tgacgaatac
      481 gcctgccgtg cttcacaatg gggcacggca gattcgtttt acgattctgg aatcaggttt
      541 taggagcaag ggccgtcgaa tcaataccct tctggtatgt tcctgaaccc tgaacccatc
      601 atggttttat gctcacgatt ggccaggggt ttttatgcta acaatccaaa aacttgttgc
      661 gttctcgctc cttttgttcc tcagatctgg tggtgtcgta tctggcatac aaccggcgcc
      721 gtcatcacca caattttcca atcaacttgc tttgcaagct ccggttcttg tccctggcaa
      781 aatcattaac caggaactca aaggtggaga ggtcaatcgt ttccagattc gtttaaaagc
      841 ccggcaattc cttgatttga gtgttgagca gcagggaatt gatgtcgtag tccggttact
      901 tggaccagat ggaaagcgag taaccgaagt tgatagccca aatggtcctg atggcccgga
      961 accagtcgtg ctgattgtga ggcaatcagg aacctacact cttgagatca aagcccctga
     1021 cccggctgcc gagccgggca agtatcagat cacctgtgaa cccttgcgcc gggcaacgcc
     1081 g
//
LOCUS       DAOQDO010002483         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_186887_length_1080_cov_2.794146, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002483 DAOQDO010000000
VERSION     DAOQDO010002483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186887_length_1080_cov_2.794146"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..345
                     /locus_tag="PKZ53_28165"
                     /pseudo
     CDS             88..345
                     /locus_tag="PKZ53_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100714.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            436..813
                     /locus_tag="PKZ53_28170"
     CDS             436..813
                     /locus_tag="PKZ53_28170"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ44390.1"
                     /translation="MDQSRDLNTAFRLAARYNFFETLRQLLDLGADINGQSASNGSTA
                     LMYAVEHRNLEMVQFLIDHNANVQLCNHIGDNVIDACPDWKNRAEIYQLLLTAGARPK
                     KGSSTASPRTGGCCHHHPEEPAQ"
     gene            848..>1080
                     /locus_tag="PKZ53_28175"
     CDS             848..>1080
                     /locus_tag="PKZ53_28175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44391.1"
                     /translation="MSVFPSNLFPLACSPMVELTQILGGPLWFYQSAGLILSAILLKS
                     LTFAWLEKSYQAFWLMLAGNIASSMVVGLSFPLT"
BASE COUNT          260 a          269 c          252 g          299 t
ORIGIN      
        1 tgaatgcctc cttcggtgtg aaaaaacagg gccgtccaca aaaccccagt ttcgtggacg
       61 gcctttttct gtttgcaaag gagctttatg ttgtatcttg aaaacaatgc ttcagaaaaa
      121 gccagcgttg aacatcgtta cgcggaaaat gttcctggac cgttttatgt ggacaatcag
      181 tgcctggatt gtgatgtgtg ccggtatttt gctccatcaa atttcacccg tcaggacgat
      241 agtgggtact cttttgtcta caaacaacct gaaaacatcg aagaactcct tgcctgtatt
      301 gaggtactgc aagcctgtcc ctgtgttgcc attggtattg atggcggtcc cttgagcgag
      361 caatttcctg agcctgacct gattctgttc aaagaattac aaaagacgcc gacctctcaa
      421 gcaattatca ggtatatgga ccagagccgc gatctgaaca cggcctttcg actggctgcc
      481 cggtacaact tctttgaaac actcaggcaa cttcttgatt taggagcaga tatcaatggc
      541 caatctgctt cgaatggttc aaccgccttg atgtatgccg tcgagcaccg caaccttgaa
      601 atggttcaat ttctgattga tcacaatgca aatgtccagc tctgtaacca tatcggtgac
      661 aatgtaattg acgcctgtcc cgattggaaa aaccgtgctg aaatatacca gcttttgctg
      721 acggctgggg ctcgtccaaa aaaaggcagt tcgacggcaa gtccccgcac tggtgggtgt
      781 tgtcaccatc atcctgaaga accagctcaa tgaaccaatc tcaatgtgag agttctggag
      841 atttcccatg agcgtttttc cgtcaaatct tttcccactg gcttgctcac cgatggtgga
      901 actgacccaa attctgggcg gccccctctg gttttatcaa tccgccgggc tgatcttgag
      961 tgccatcctc ttgaaatcac tcacgtttgc ctggcttgaa aagtcttatc aggcgttttg
     1021 gctcatgctg gccggaaaca tagccagttc gatggtggtt gggctgtcat ttccgctgac
//
LOCUS       DAOQDO010002484         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_186957_length_1080_cov_2.265366, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002484 DAOQDO010000000
VERSION     DAOQDO010002484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186957_length_1080_cov_2.265366"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>1080
                     /locus_tag="PKZ53_28180"
     CDS             219..>1080
                     /locus_tag="PKZ53_28180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44392.1"
                     /translation="MKELYWPEFETTLKQKAYAQIAQSPELLNQFRKRKLTGMLQNLL
                     SWLVYGLGTFIFGLGTLVVFSLFTVPKGSDAQAFWFLLPVAATCASIAWASFVRDKLQ
                     VSPAHLVFCGLPIPNPGFFNCIWSKTHLFQAALFILFSAATLGSIWGRQPVRHSILNF
                     IHPALQIFIGLGVAGLFTLITQSSTVWLLRGWSTYLSKTLRYVLFPWIIFVLGIAIGL
                     NPFHLSPLPGDILEFSTLISPVGWVCYAFSHGLIDLDAQCLFALVPAGLFLVEKSRRF
                     QQLRASFSLEG"
BASE COUNT          220 a          267 c          259 g          334 t
ORIGIN      
        1 ttgcagccgg ctcaacctgg gagtttgcca cggtctggct gatcttcatg ggcttaatgt
       61 ttctctggct actggcgttt ggaaagttat acaaccacgg aaaagttgat ttgctccgaa
      121 aagcagcttg aggattgaca gttttcaggc tgtcaccttg tcacccagcc accttgtcac
      181 ccagtcaccc ggtcacttca ccgtcgaggt tcaaagccat gaaagaactg tactggcctg
      241 aatttgaaac aactctcaag caaaaagctt atgcccaaat cgctcaatca ccagagcttt
      301 tgaatcagtt ccgcaagcgg aaactgacgg ggatgctcca gaatcttctg agctggcttg
      361 tatatgggtt ggggactttt atttttgggc tggggacgct ggtggtgttt tctttgttta
      421 ccgttccaaa agggagtgat gcgcaggcgt tctggttttt gcttccggtt gcggccacct
      481 gcgcgagcat tgcctgggcc tcctttgttc gagataaatt acaggtctca ccagcccacc
      541 tggttttttg tggcctccca attccaaatc ctggcttttt caattgtatc tggagcaaaa
      601 ctcacctttt tcaagccgca ctgtttattc ttttcagcgc cgctacattg ggaagtatat
      661 ggggaaggca accagtacgg cattcaattc ttaatttcat ccatccagcg ctccagattt
      721 tcattggact tggggtagct ggactcttca cgttgatcac tcagagcagt accgtgtggt
      781 tgcttcgtgg ctggtcaaca tacctttcga aaactctgcg gtatgtcttg ttcccctgga
      841 ttatttttgt attgggtata gcaattgggc tcaacccatt tcatctgagt cctttaccgg
      901 gagacatcct agagttttca accctgatct ccccggttgg gtgggtttgt tatgcctttt
      961 cccacggcct gatcgattta gatgctcagt gtctttttgc tctggtccca gccggtttgt
     1021 ttcttgtaga aaaatctcgc cggttccagc aactgcgagc atcgttttca cttgaagggc
//
LOCUS       DAOQDO010002485         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_187222_length_1079_cov_3.081055, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002485 DAOQDO010000000
VERSION     DAOQDO010002485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187222_length_1079_cov_3.081055"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKZ53_28185"
     CDS             complement(<1..181)
                     /locus_tag="PKZ53_28185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44393.1"
                     /translation="MKRCPSCNSIFQSQQLFCPQDGRELEPGCVLDGKYQLDDLIGEG
                     GMGQVYRATHIHIGTQ"
     gene            273..>1079
                     /locus_tag="PKZ53_28190"
     CDS             273..>1079
                     /locus_tag="PKZ53_28190"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ44394.1"
                     /translation="MVILAETFCGSGTNFQALAQSGGATTGELTGLVIDVRGVGVAGV
                     NLTAQRVETGLVRQTQTDPSGQFRLVQLPPGLYQVTVEAAGFHKQTSEVTVSIGTSET
                     VDFQLAIEGTQEVMEVTASDDEFRKNQVRRKTESSTIINQLLVNNLPINQRDFLDFSR
                     ISARVTEDRIPSQGVSTTSKLSFNGQASRFNNINVDGLDNNDAGVGAGRSTFSQESVR
                     EFQVISDSFSAEFGRSLGGIINIVTRGGSNEFHGTLFGFFRNEALSARDAF"
BASE COUNT          257 a          250 c          294 g          278 t
ORIGIN      
        1 attgtgtccc aatatgaatg tgggtagccc gatacacctg ccccattccg ccttcaccaa
       61 tcaggtcatc aagttgatat ttcccatcca gaacacatcc gggttccagt tctcgtccat
      121 cctgcggaca gaacagttgc tgactttgga aaatgctgtt gcaggatgga caccgtttca
      181 tagtgatttt gagttcagaa ccagcacagg accaggcata cccaaaggga gttattgcaa
      241 tgagatgtta tgtgaggttg tggatttggt gtgtggtcat cttagcagaa acattttgtg
      301 gaagtggaac caatttccag gctctggctc agtcgggagg tgcgaccaca ggtgaactga
      361 cgggtttggt gattgatgtt cgaggcgtgg gtgttgccgg agtgaatttg acggcgcaac
      421 gcgtcgaaac cggcctggtt cggcagaccc aaactgatcc ttcaggccag ttccggttgg
      481 tgcaattgcc gccagggtta tatcaggtga cagttgaagc tgccgggttt cacaaacaga
      541 catcggaggt gacggtttcg attgggacat ccgaaaccgt tgatttccag cttgcgattg
      601 aaggaacgca agaagtgatg gaagtaacgg cctccgacga cgaattccga aaaaaccagg
      661 ttcggcgcaa gaccgaaagc agcaccatca ttaaccagtt actggtcaat aatttgccaa
      721 tcaaccagcg ggattttctg gatttttccc ggatttcagc ccgtgtaacc gaagatcgaa
      781 tcccatcaca aggagtttca accacctcga agctttcctt caacgggcag gcttcgcgct
      841 ttaacaatat caatgtggat ggtctcgaca acaatgacgc tggcgttggg gctggtcggt
      901 caaccttcag tcaggaatcc gtccgcgagt ttcaggtgat ttccgatagt ttttcggctg
      961 aatttgggcg ttcgcttggt ggaattatca atattgtcac ccgaggtggg agcaatgagt
     1021 ttcacggcac actctttggg tttttccgta atgaggcact cagtgcgcgg gatgctttc
//
LOCUS       DAOQDO010002486         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_188211_length_1076_cov_3.153771, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002486 DAOQDO010000000
VERSION     DAOQDO010002486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188211_length_1076_cov_3.153771"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..>1076
                     /locus_tag="PKZ53_28195"
     CDS             144..>1076
                     /locus_tag="PKZ53_28195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ44395.1"
                     /translation="MKLPLPSDNFLLPDPEYLLAENAELRQQLEEARVQLELQRERVE
                     ELESSVFFKLKQRVVRWLNACGLSEQEAWQAHQRQGIAGIVQVILRLNLKRWWQSLGG
                     RFRPNPNYEYDQWARGLFPGNDACRDLERDCRQWKYQPTISLLMPVYNPLESFLVEAL
                     ESVQAQIYPFWELCIVDDASTMPYVRPVLEDFAAREPRIKLRFLKRNGHIAHASNAAL
                     ELATGEFVGLVDHDDMLTVDALFEVAKRLNMSPATDFLYSDEDKLNESGQFSEPYFKP
                     DWSPELFLSQMFTGHFSVYRRELVSRVGGFRVGFE"
BASE COUNT          250 a          265 c          285 g          276 t
ORIGIN      
        1 agtcttcagc ccccagccca gggtcttcag cccaaggtct tcagcccaaa gtcttcagcc
       61 caaggtcttc agcccaaggt cttcagccca aagtcttcag cccgaagtct tcagccccaa
      121 gcccgaggtt ttcagcccta aaaatgaaat taccgttgcc atctgacaat ttcttgctgc
      181 ctgatccaga atacctgctc gctgagaatg ctgaactcag gcagcaactt gaggaggcta
      241 gggtccaact tgaactccag cgtgaacgag ttgaggaact ggaatcatct gtttttttca
      301 aattaaaaca gcgggtggtt cgctggttga acgcctgtgg tttatctgag caggaagcct
      361 ggcaggccca tcaacgacag ggaatcgcag ggattgtgca ggttattctg cgtctcaatc
      421 taaaacgatg gtggcaatcg ctgggaggtc gctttcgccc aaatccaaac tatgagtatg
      481 accagtgggc ccgggggctt tttccaggga acgatgcctg ccgggatctt gaacgtgact
      541 gccgccagtg gaagtaccag ccgacgatca gtttgttaat gccggtgtac aatccgctgg
      601 aatcttttct ggtcgaagcg ctggaatcgg tccaggcaca aatttatcca ttttgggaat
      661 tgtgcattgt ggatgatgct tcgacgatgc cgtatgtaag gccagtgctt gaagattttg
      721 ccgcccgtga accgcgcatc aaactcaggt ttcttaaaag aaacgggcat attgcgcacg
      781 cttcaaacgc tgccttagaa ctggccaccg gtgaatttgt tgggctggtg gatcacgatg
      841 atatgctgac ggttgatgct ttgtttgaag tcgccaaaag gctcaacatg tcaccagcga
      901 ctgatttttt gtactcagat gaagacaaac tcaacgagtc tggccagttc agtgagccat
      961 atttcaaacc ggactggtcg cctgaattgt ttctatcgca aatgtttacc ggccatttta
     1021 gtgtctatcg gcgggaactc gtcagccgcg tgggtgggtt tcgcgtcggg tttgag
//
LOCUS       DAOQDO010002487         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_188218_length_1076_cov_2.997062, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002487 DAOQDO010000000
VERSION     DAOQDO010002487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188218_length_1076_cov_2.997062"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKZ53_28200"
     CDS             complement(<1..721)
                     /locus_tag="PKZ53_28200"
                     /inference="COORDINATES: protein motif:HMM:NF022649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite-like stress resistance cysteine
                     protease StiP"
                     /protein_id="HNJ44396.1"
                     /translation="MFIDALSRAGRPNYWNQPLCADLHREVDPFTELELFRPLTKAYD
                     GQTTFWEIPSPEPDLPDSFWTLLATHLTDIRLQEAGARLAASLLQWESHPEKMVLVAI
                     LRAGVPIADWLTRLLPGAVAVQTSLFSGLGIDQMALNHIKHQFPERRIVFVDGWTGRG
                     GVARELARWESGPLAVLFDPWGWADFCGAAEDIFSPSACFTGPSTLGFSRTFFVGPDQ
                     LFGAYAFPQTYIRKPLIDQWQA"
     gene            complement(844..>1076)
                     /locus_tag="PKZ53_28205"
     CDS             complement(844..>1076)
                     /locus_tag="PKZ53_28205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44397.1"
                     /translation="TRNPHLSIRHVTLSPWKIDHQNVFDRIQINGRYYVYNLGEFQGP
                     VHILSDPFDHASALSLQETMFERGYETKVLAG"
BASE COUNT          289 a          280 c          286 g          221 t
ORIGIN      
        1 acgcctgcca ctggtcaatc aaaggttttc gaatataggt ttgcggaaac gcataggccc
       61 caaaaagctg atctgggcca acaaagaacg ttcgcgaaaa cccaagtgtt gacggcccgg
      121 taaaacaggc cgaaggtgaa aagatatctt ctgccgcgcc acaaaaatcg gcccaacccc
      181 aggggtcaaa aagtacggcc agggggccag attcccagcg ggccagttca cgggccacac
      241 ctccgcgccc tgtccagccg tctacaaaaa cgattcggcg ctcaggaaac tggtgtttga
      301 tgtggttcag ggccatctga tcaatcccaa gaccagaaaa aagtgaggtt tggaccgcaa
      361 cggcgccagg caataaccgc gtcaaccagt ctgcaatcgg aactccagcc cgtaaaatag
      421 ccaccagcac cattttttcg gggtgtgatt cccattgtaa aaggctggcg gctaaccggg
      481 cgccagcctc ttgcaggcgg atatcggtca gatgagttgc cagcaacgtc cagaacgaat
      541 ccggtaaatc cggctcaggt gatggaatct cccaaaatgt cgtttgcccg tcataggctt
      601 tcgtcaacgg gcgaaacaat tccagttcag taaatgggtc aacttcgcgg tgcagatcag
      661 cacacagtgg ttgattccag taatttggcc gacctgcgcg gctgagagca tcaataaaca
      721 ttcgggttca gggttcaggg ttcagggttc cctgagtatg gaaaatatgc gtcataccaa
      781 agaagctaaa atttgagaaa ccagcgaagt ctgagcctgc cagcaaacca gagccgactg
      841 ggtttagccg gctaaaacct tcgtttcgta cccgcgttca aacatcgttt cctgaagcga
      901 gagggctgat gcgtggtcaa aggggtcact cagaatatgc accgggccct gaaattcacc
      961 caggttatag acataatacc ggccattgat ttggatccgg tcaaacacgt tttggtgatc
     1021 aattttccag ggactcagcg tcacgtggcg aattgacaga tgcggattgc gggtca
//
LOCUS       DAOQDO010002488         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_188725_length_1075_cov_1.593137, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002488 DAOQDO010000000
VERSION     DAOQDO010002488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188725_length_1075_cov_1.593137"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..>1075)
                     /locus_tag="PKZ53_28210"
     CDS             complement(30..>1075)
                     /locus_tag="PKZ53_28210"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ44398.1"
                     /translation="SIVMTQDLEDLAGMVLQPLAGKIGRGRVLIVPDGALQYIPFGCL
                     PVKKASRSRQTTSRLLVAQNEIVMLPSASALSILRKETAGRVTKPVSVAIVADPVFDP
                     RDERFQGKSTADSQSPTESVTVSAQKRIYNRILEREKENSSTSEGAQDVVNLRIPRLP
                     FTRVEADQISKLVGAENSIRLVSFAANRQAITSGELSKYPYIHLATHGLIDADRPDLS
                     ALVFSLIDETGKPVDGYLRALDIYNLSLPAELVVLSACETGLGKQIKGEGMVGLTRGF
                     MYAGAKRVIVSLWSVNDEATASLMTSFYRKMIRQKQSPAAALRAAQLEILKNPKWKAP
                     FYWAPFMIQGEFR"
BASE COUNT          245 a          300 c          256 g          274 t
ORIGIN      
        1 ttcattcttc attcttcact cttcattcct taccggaatt ccccctgaat cataaacggt
       61 gcccagtaaa acggagcctt ccactttgga tttttgagta tctccagttg agccgcacgc
      121 aaggcggcgg ctggagattg tttctggcgg atcatttttc gatagaaact ggtcatcaag
      181 ctggcagtgg cttcgtcgtt gacactccac aggctgacga taacccgttt tgcaccggcg
      241 tacatgaacc cacgcgtcaa tccgaccatc ccctcgccct taatctgttt cccaagcccg
      301 gtttcgcagg cggagagcac caccagttcg gctggaagcg acaggttata gatatcaagc
      361 gcccgtaaat acccatctac cggttttcca gtttcatcaa tcagtgaaaa aaccagagct
      421 gataaatcag gccggtcagc atcaattaac ccgtgagttg ccaggtggat atagggatat
      481 ttacttaact ctccgctggt aattgcttgc cgattggcgg caaaacttac cagacggatg
      541 ctgttttcag cgccgaccag tttggaaatt tgatcagctt cgacacgcgt gaatggtagc
      601 cgggggatcc gaaggttcac aacatcctgt gctccttcag atgttgaact gttctccttt
      661 tcacgttcca aaatgcggtt atagatgcgc ttttgggctg acaccgtcac cgattcagtt
      721 ggcgattgcg aatcagccgt tgattttccc tgaaatcgtt catcgcgtgg gtcaaagacc
      781 gggtcagcca cgatggccac cgagactggt ttagtgactc ttccggctgt ttcttttctc
      841 agaattgaga gcgccgaggc cgatggcaac attacgattt cgttctgagc caccagcagc
      901 cgcgaagtag tttgcctgga acgcgacgct tttttcaccg gcaggcagcc aaacgggatg
      961 tattgcaatg ccccatcggg cacaatcagt acccgtccac gaccaatctt ccccgccagc
     1021 ggctgaagca ccatcccggc caggtcttcc aaatcctggg tcatcacaat tgaca
//
LOCUS       DAOQDO010002489         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_188939_length_1074_cov_2.218842, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002489 DAOQDO010000000
VERSION     DAOQDO010002489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188939_length_1074_cov_2.218842"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKZ53_28215"
     CDS             complement(<1..777)
                     /locus_tag="PKZ53_28215"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HNJ44399.1"
                     /translation="MSSTFDTQELTMLHAVASQYPTTDDALAEISILKAGLSLPKGVV
                     HIVSDVHGEYKKLRHIINNASGSLRPLVETLFASRLSAEEQQQLLATIYYPHEVIEHL
                     RPRFQESGARHRWVRHTIREQFEIVLALGRNYRRRHVTSLFPREFQELFEELLDESAA
                     GRGPGYINAMLDALQPYDRDLNAVRAASRLIRNLSVAELVVAGDLGDRGPRLDKVLDY
                     LMQQPNVSFTWGNHDASWMGACLGNEALIATVLRISLRYRR"
     gene            complement(874..>1074)
                     /locus_tag="PKZ53_28220"
     CDS             complement(874..>1074)
                     /locus_tag="PKZ53_28220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44400.1"
                     /translation="RTRLRSITEEKSEVSDDEWLKAKTEFKNELAGFTLGWNSTCGPE
                     ARVQPFRRHFFCSFTCLLLVIS"
BASE COUNT          273 a          264 c          285 g          252 t
ORIGIN      
        1 ccgccgatac cggagtgaaa tccgtaaaac ggtggcgatg agggcttcat tgccaaggca
       61 ggcacccatc cacgacgcat cgtggttgcc ccaggtgaaa gaaacattcg gctgttgcat
      121 gagataatca agcactttat ccaatcttgg accccggtca cccaaatcgc cagccacaac
      181 cagttcggcc acagaaagat tgcgtatcag gcgtgatgcg gctctgacgg catttaaatc
      241 gcggtcatag ggttggagtg catccagcat ggcattgata tagcccggtc cgcgacctgc
      301 ggctgattca tcaagcagct cttcaaacag ttcctggaac tcacgtggaa aaagcgacgt
      361 gacgtgccgg cgacgatagt ttcgacccag tgccagcacg atctcaaatt gttcccggat
      421 tgtgtgtcga acccagcgat gccgggcgcc gctttcttga aaccggggcc gaaggtgttc
      481 gatgacttcg tggggataat aaatagtggc gagcaattgc tgttgctcct cagccgacaa
      541 acgactggca aagagggttt cgaccagagg ccgaagactg cctgaggcat tgttgataat
      601 gtggcggagc tttttatatt caccatggac atcgctcacg atgtggacca cgccttttgg
      661 aagcgagagc ccggccttca gaatcgaaat ttccgccagg gcgtcatccg tggttggata
      721 ttgggacgcc accgcgtgca acatggtcag ttcctgggtg tcgaaggtcg aagacataaa
      781 atgtttcctc agagtcggat gggatgaagg tgaattgtga gtagtcaaaa gtcagaagtg
      841 gcaggtcaac gagcgatagt atttcatttc atatcaactg attacaagta aaagacaggt
      901 gaaggagcag aaaaaatgac gcctgaaagg ttgaacgcga gcttccggac cacaggtact
      961 gttccaaccc aatgtaaacc ctgcaagctc atttttaaat tctgtttttg ctttgagcca
     1021 ctcatcatca ctgacttctg atttttcttc tgtgatactt ctcaacctcg tccg
//
LOCUS       DAOQDO010002490         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_189185_length_1073_cov_3.718075, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002490 DAOQDO010000000
VERSION     DAOQDO010002490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189185_length_1073_cov_3.718075"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PKZ53_28225"
     CDS             <1..991
                     /locus_tag="PKZ53_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ44401.1"
                     /translation="AFDPKERAFTRNAEFPLDADVVIVDEASMLDIVLASHLLKAVPS
                     TGQLVLVGDVDQLPSVGPGRMLADVIDSKSIPVIRLTQVFRQAQGSLIITNAHRIRDG
                     HFPRLIAPGGAEVTDCYYLEAEEPEDIHKLLINAVTRSLPKRFGYDPLADIQVLTPMN
                     RGSVGAAELNLILQQHLNPFASNKEQIEKMGRVFREGDKVIQKVNNYQLEVFNGDVGL
                     IRNIDTENHTVWIEFSEREVEYDWADLNELGHGFAMSVHKSQGSEYAAVVMILHAQAW
                     PVLSRNLVYTGLTRAKKTAVMIGNKRAIGAAMRKMEASTRNTRLAEILPLPIE"
BASE COUNT          281 a          270 c          281 g          241 t
ORIGIN      
        1 ggcatttgac cccaaagaac gcgcctttac gcgcaatgct gaattcccgc ttgatgccga
       61 tgtggtgatt gttgatgaag cgtcaatgct tgatatcgtg ctggcttcgc acctgctcaa
      121 agcggttcca tccactggac aactggtgct ggtgggcgac gttgatcagt tgccatcggt
      181 tggtccaggc cgaatgctgg ccgatgtgat tgactccaaa tcaattccgg tcatccgact
      241 gacccaggtg tttcgtcagg cgcaagggag tctcatcatt accaatgccc atcggattcg
      301 ggacggacac tttccgcgat tgattgcccc cggtggtgca gaagtcaccg actgttatta
      361 tcttgaagct gaagaacccg aagatattca caagctcctg atcaatgccg tcactcggag
      421 cctccccaaa cggtttggat atgacccgct ggctgacatc caggtattga ccccaatgaa
      481 ccggggttcg gtgggggcgg ctgaactcaa tttgatcttg cagcaacacc tcaatccgtt
      541 tgcttcaaac aaagagcaaa tcgagaaaat gggtcgtgtc tttcgcgaag gtgacaaggt
      601 catccagaag gtcaacaatt atcaactcga agtgtttaat ggagatgtgg gcctgattcg
      661 caacattgac actgaaaatc acaccgtctg gattgaattc tccgagcgtg aagtcgagta
      721 tgactgggcc gatttaaacg aactggggca cggttttgcc atgtcggttc acaaaagcca
      781 ggggtcggaa tatgccgccg tggtgatgat cctccacgcg caggcctggc cagtgctcag
      841 ccggaatctg gtgtataccg gactcaccag agccaaaaaa accgctgtca tgatcggcaa
      901 caagcgcgcc attggcgccg cgatgcgcaa gatggaagcc agtacccgca acacccggct
      961 cgccgaaatt ttgccgctac cgattgaatg aagaattgag aatgaagaat gaagatttta
     1021 tagaatttca gataaagaaa ccacggaata cacggaacac acagaaaata aat
//
LOCUS       DAOQDO010002491         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_189228_length_1073_cov_2.660118, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002491 DAOQDO010000000
VERSION     DAOQDO010002491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189228_length_1073_cov_2.660118"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..918)
                     /locus_tag="PKZ53_28230"
     CDS             complement(31..918)
                     /locus_tag="PKZ53_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44402.1"
                     /translation="MTKPIFEIVDELPSSGMTVSLLNALDFVVPGQWQNIVGFENTIR
                     TVTGVTDQNVIQQIGERAIHLYNDPAEGYQRAMWLYQVANSAGGMIGSAAMANKVTES
                     VSFLGFLNRLTPKADKLQAIDFSLKIVVELIAYTQINGIPGDSIGDFVNGLADYGNES
                     LMRMAALVCVDGLVPLGPDFLSSVGSTIRAVSAYDLDSNPVFSKISSYIPGGDTIGKL
                     GFVGQSFDSVRSWMEGFVASRGLTPQMITGNLQRYIDITDDKLDYLAAFLDMTTNYYE
                     HTGVQTLARRLVERAVAEI"
BASE COUNT          298 a          277 c          227 g          271 t
ORIGIN      
        1 ccgatgtgtt ccaatctcct gtggttcgat ttagatttca gccacagcac gttcaaccag
       61 acgacgcgcc agggtttgca ccccggtgtg ttcataataa ttggtcgtca tatcaaggaa
      121 agccgccaag tagtccagct tgtcatcggt gatatcaatg tagcgctgca aattcccggt
      181 aatcatctgt ggtgtcagac cacgcgatgc aacaaagcct tccatccagc tacggaccga
      241 atcaaaactc tgtccgacaa atcccagttt tccgatggtg tcaccacctg ggatgtagct
      301 actgatcttg ctaaacaccg ggtttgaatc aagatcataa gcagaaaccg cccgaatggt
      361 tgacccaact gaactcaaaa agtccggccc aagtggaacc aatccatcca cacagaccag
      421 ggccgccatt cgcatcaagg attcattgcc ataatcggcc aacccattga caaaatcgcc
      481 gatgctatcg ccaggaatgc cattgatttg agtgtacgca atgagttcga cgacaatctt
      541 gagcgagaaa tcaattgcct gcaatttatc ggcttttggt gtcaaccgat tcaaaaaccc
      601 taaaaacgaa acgctttcgg taactttatt ggccatggcg gctgatccaa tcatcccgcc
      661 agcactgttg gcgacctgat atagccacat cgcccgttga tacccttcag ccgggtcgtt
      721 atagagatga atggcgcgtt cgccaatctg ctgaatcaca ttctgatcgg tcacgcccgt
      781 aaccgtgcga atcgtatttt caaaacccac gatgttttgc cattgtcccg gaacaacaaa
      841 gtccaaagcg ttaagcaatg ataccgtcat tccactggaa ggcagttcgt caacaatttc
      901 aaaaatcggc ttggtcattg aaccagatct ccttgattga atatttggtt tcgttcaacg
      961 gttcatattc gaaatgaaaa atgaatcaag gtgaagcgtg aaaaattcgt gagtcgggca
     1021 aacgttctga aaaagagatt tcatgcttca tgcttcattc ttcatcctat ctt
//
LOCUS       DAOQDO010002492         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_189608_length_1072_cov_2.653884, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002492 DAOQDO010000000
VERSION     DAOQDO010002492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189608_length_1072_cov_2.653884"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1072
                     /locus_tag="PKZ53_28235"
     CDS             <1..>1072
                     /locus_tag="PKZ53_28235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44403.1"
                     /translation="AMTTIGQFSFQASIKPDATTQPRVLSPAFPKSQPASGQTLTPDE
                     AVIAAVNHLEATDTGWMLKNPQHWAEFTAEGITFRPRHGGPEWKWKLSAVGTTQVKTP
                     LVRLQEVMPVNESAEDVAFHRLGMTERYLAKARTLEQQFVLLQPLDLGREDLVIAGQI
                     DCAGDFSREEKQWVWKTGNGAVTLGDVLVYDADGKELEAHMEVSSNGTKIVVANSGLA
                     AATYPVTIDPEIGTNDFRISDMGPDGDANFDALNASVAYNSTNNEYLVVWDGEDNTSP
                     LVDGESEIFGQRVNAATGAEIGVNDFRISDMGPNGDAAYDAFNPSVAYNSTNNEYLVV
                     WSGDDNTAPFVDNEFEIYGQRLN"
BASE COUNT          281 a          253 c          298 g          240 t
ORIGIN      
        1 gccatgacaa cgattggaca attttcattt caagcttcga tcaaaccaga tgcaactacc
       61 cagccgcgag ttctttcgcc ggctttccca aaatctcaac ctgcatcggg acaaactctg
      121 actccagatg aagccgtgat tgctgctgta aaccatctgg aggccactga cactggctgg
      181 atgctcaaga acccgcagca ctgggctgaa tttactgctg aggggattac tttccggcca
      241 cgccacggcg gtccagaatg gaaatggaag ctgagtgccg tcgggaccac ccaggtgaaa
      301 acaccactgg ttcgccttca ggaagtaatg ccggtcaatg aatcggcaga ggatgtggcc
      361 tttcaccggc tgggaatgac tgaacgatat ctggccaaag cccgaaccct tgagcaacag
      421 tttgtgctgc tccaaccact tgaccttggc cgtgaagacc tggtgattgc cgggcagatt
      481 gactgtgccg gggacttcag ccgggaagag aaacagtggg tgtggaaaac tgggaacggg
      541 gccgtcacgc tcggtgatgt cctggtctat gatgctgacg gaaaagagct tgaagcccac
      601 atggaagtca gttctaatgg gacaaaaatt gtggtggcaa acagtggtct ggctgctgcc
      661 acatacccgg tgacgattga tccagaaatt ggaaccaatg attttcgaat cagcgatatg
      721 ggaccggatg gagatgccaa ttttgatgcc ttgaatgcct ccgtggccta taacagcacc
      781 aacaatgaat atctggtggt atgggacgga gaggacaata cgtctccatt ggttgacggt
      841 gaaagtgaaa ttttcggaca acgggtaaat gcagcgaccg gagccgagat tggcgtgaat
      901 gattttcgaa tcagcgatat ggggccaaac ggagatgcgg cttatgatgc gtttaacccg
      961 tcagttgcct ataacagcac caacaacgaa tatctggtgg tttggtccgg cgatgacaat
     1021 accgctcctt ttgtcgataa tgaattcgag atttatggac aacgactcaa tg
//
LOCUS       DAOQDO010002493         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_189827_length_1072_cov_1.581121, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002493 DAOQDO010000000
VERSION     DAOQDO010002493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189827_length_1072_cov_1.581121"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKZ53_28240"
     CDS             <1..210
                     /locus_tag="PKZ53_28240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44404.1"
                     /translation="VFVNNFVCFHARTAFTDDQTDEAHRRHLSRIWLSMPNSRPLSPR
                     YKHYFFQNTQAGALRGGLPLPEVSY"
     gene            429..>1072
                     /locus_tag="PKZ53_28245"
     CDS             429..>1072
                     /locus_tag="PKZ53_28245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ44405.1"
                     /translation="MNSIGIDVRHLLDFSLPELTRKTALVYQDRTYTYQDLSNEVSRL
                     AYGLLQTSSPGDLLAIWLPNCPELLCLYLACLKTGIVPMPVHQAMTWPEVHQILGSTR
                     THTLITSPKILTKWPVDCATTSLEQIYVLELDQRSAFFRPYDELTASAWHDCTVPHLS
                     ENSPGFVLHTSGSEGYAKGVMLSYRNLNHILNFRLGHTRLTPESISVVASCLTQ"
BASE COUNT          250 a          262 c          256 g          304 t
ORIGIN      
        1 gtgtttgtga ataatttcgt ctgctttcat gctcgaacgg catttaccga tgatcaaaca
       61 gacgaggcac atcgtcggca tttgagtcga atctggcttt cgatgcccaa ttcacgccct
      121 ttatcgccac ggtacaaaca ctatttcttt caaaacaccc aggccggagc gcttcgcggg
      181 gggcttccac tgcctgaggt gagctattga tacgtacttt gtcaaaagtt tcctgagcta
      241 ttgaatgttg ggagtgtgtg gttcttttcg tgtatttcgt gtatttcgtg gttgagaatg
      301 tctggaaact ttcggtaagg tacttatacc gttttggaac gaagctatcc tatcagcaaa
      361 aggctaaatc attgggaaaa ttggatttcc ctgacccaga taccctgatc ccaaaatggt
      421 gtaacaccat gaactcgatt ggaattgacg tgcgccatct tcttgatttc agccttccgg
      481 aattgaccag gaaaaccgcg ctcgtttatc aggatcggac ttatacgtat caagatctat
      541 caaatgaagt ttcgcggctg gcgtacgggt tgttgcaaac atcgagccct ggtgacctgc
      601 tggcaatctg gctgccaaat tgtccggaac tcctgtgcct gtacctggct tgtttgaaaa
      661 ccggaattgt cccgatgccc gttcatcagg caatgacctg gcccgaagtg catcagattc
      721 tgggttccac ccgtacccac accttaatca cctcaccaaa aatccttacc aagtggccag
      781 tggattgtgc cacgaccagc cttgagcaga tttatgtcct tgagttagat caacggtcgg
      841 cgttctttcg cccgtatgat gagttgaccg ccagtgcctg gcatgattgt acggttccac
      901 acctttcaga aaacagtcca gggtttgtat tgcacacttc agggtctgaa gggtatgcca
      961 aaggcgtgat gctttcgtac cgtaacctca atcatattct gaatttccgg cttggtcata
     1021 ccagactcac gccagagagt atcagtgttg tggcgtcgtg tttgactcaa ag
//
LOCUS       DAOQDO010002494         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_189928_length_1071_cov_3.380906, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002494 DAOQDO010000000
VERSION     DAOQDO010002494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189928_length_1071_cov_3.380906"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="PKZ53_28250"
     CDS             <1..>1071
                     /locus_tag="PKZ53_28250"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNJ44406.1"
                     /translation="IWIHAVSVGEALAARPLAMALRTCYPQARIVISTTTETGQQRAR
                     EQLAMLDGHFYFPLDVPGVVTRALDHIQPSLVIILETEIWPNFLRACARRSIPVVLVN
                     GRLSDRSFGRYHRVRRWLQPVLALFTRMLMQSNGDAERILALGADPDRVRMIGNLKYE
                     PPDAGEVTRRDRVARELDAQFGLSSSPGPLLVAGSTVEGEEPMLVKAFEQLRSCPGLE
                     FLRLLIVPRHPERFEAVNQILSQAGGPIARRSKPSTTDSTASVMMLDSIGELAAVYRF
                     ATVVFVGGSLVPRGGHNILEPAADGRAVVTGPFTENFRDILATFVAAQAVKQLPPDTP
                     DGLIQRFVTLCEELLLNAESRHS"
BASE COUNT          200 a          293 c          308 g          270 t
ORIGIN      
        1 atttggattc atgctgtttc tgtcggagaa gctctggcgg cgcgtccgct ggcaatggcg
       61 ttgcggactt gctatccgca agcccggatc gtgatttcaa ccacgactga aactggacaa
      121 caacgtgccc gtgagcaact tgccatgctc gacgggcatt tttatttccc cctggatgtg
      181 ccaggagtgg tcacccgggc acttgatcac attcaacctt cactcgtcat cattttggaa
      241 accgaaatct ggcctaattt cctgcgggcc tgtgcccggc ggtcaattcc cgtggtgctg
      301 gtcaatggcc ggctttcaga ccggtctttc ggtcgctatc accgggtgcg tcgctggttg
      361 cagccggttc tggcgctttt cacccggatg ctgatgcaat caaacggtga tgcggaacgc
      421 attctggcgc ttggtgccga tccagatcgt gtccgaatga tcggcaattt gaagtatgaa
      481 ccgcctgatg ctggcgaagt cacccgccgg gatcgggtag cccgtgaact cgatgcccag
      541 tttgggctgt cgtcatcacc tggccccttg ctggtcgcag gcagtacggt tgaaggtgaa
      601 gaaccaatgc tggtcaaagc ctttgagcag ttgcgctctt gtcctgggtt ggagttttta
      661 cgccttctca ttgtgccgcg acatccagag cggtttgaag ccgtgaatca gattttgtca
      721 caggctggtg gaccaatcgc tcgcaggtca aaaccgtcaa ccactgattc caccgccagc
      781 gtgatgatgc ttgattcgat tggtgaactg gcggcggtat accggtttgc aactgtggtg
      841 tttgtcggtg gcagcctggt tccgcgaggc gggcacaaca ttctggaacc ggcggctgat
      901 ggacgagccg tggtaacggg tccttttact gagaattttc gcgacatcct ggcaacgttt
      961 gtcgcagctc aggccgtaaa acagctccct ccggatactc cggatgggct catccagcgg
     1021 tttgtcaccc tgtgtgaaga attactcctc aatgccgaat ctcgtcacag c
//
LOCUS       DAOQDO010002495         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_189960_length_1071_cov_2.735236, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002495 DAOQDO010000000
VERSION     DAOQDO010002495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189960_length_1071_cov_2.735236"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1071)
                     /locus_tag="PKZ53_28255"
     CDS             complement(<1..>1071)
                     /locus_tag="PKZ53_28255"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNJ44407.1"
                     /translation="RRRWVARMTYSQRQLQEKMTLFWHEFFSVSVHKVPGRYMVEHEA
                     LLRKNCLGNFKTILTEMSKNNAMLIWLDNNYNDGNAYDDEGNLIPPNQNFARELMQLY
                     SIGPVKLRTDGTPITDGSGVPVPSYTENDVREMARAVTGWIAGDENEDNIPPSASRFE
                     PGNHDQNNKQLFGTTLVGRKGADGVREIEDVVTLLMRQPSLPPFIAKQLIQKFASETP
                     SPAYVSRVAAAFTRTGGNIKETMRVLLLDAEFASAANIRSQYKEPTEHVIGVVRALNG
                     TTDGRKPMWVMEGCGLDLLAPPSVFSFYRPGQKISLLDTALIFNRDNGVAWILDNDFG
                     TTFDYARIIQLNQLTTPTKVVDY"
BASE COUNT          235 a          267 c          292 g          277 t
ORIGIN      
        1 gtaatcaacc actttggtcg gcgtcgtcaa ctggttgagc tggataattc gggcgtagtc
       61 aaacgtcgtc ccaaaatcat tatccagaat ccaggcgacg ccattgtcac ggttgaaaat
      121 caacgctgtg tcgagcaagg aaattttctg gcctgggcga tagaaactga acactgatgg
      181 tggcgccagc aggtcaagtc cacacccttc cattacccac attggtttgc gtccatcggt
      241 tgtgccattg agtgcccgaa cgacaccaat cacgtgttca gtcggttctt tgtactggct
      301 gcggatattg gccgcgctgg caaactcagc atccaggagc agcacccgca tggtttcttt
      361 gatattgccg ccagtccggg taaatgcagc cgcgactcgc gagacatagg ctggtgacgg
      421 tgtttcgctg gcaaactttt gaatcaattg tttggcaata aacggaggca gcgacggctg
      481 gcgcatcagg agggtaacga cgtcctcaat ttcgcgaaca ccatccgcgc ctttgcggcc
      541 aacgagggtt gttccaaaga gttgtttgtt attctggtcg tggtttcccg gttcaaatcg
      601 ggacgcgctg ggtggaatat tgtcttcatt ttcgtctccg gcgatccagc cggtgacagc
      661 gcgggccatt tcccgaacat cattttcggt atagcttggg actggaacgc cgcttccatc
      721 cgtgattggc gttccatcag tccggagttt gaccggacca atcgaataca gttgcatcaa
      781 ttcacgggca aaattctggt tgggaggaat gaggttgcct tcgtcatcat aggcattacc
      841 gtcattataa ttgttgtcca gccagatgag catggcgtta tttttggaca tttccgtgag
      901 gatggttttg aaattcccga ggcagttttt tcgcaagagc gcttcgtgtt cgaccatgta
      961 gcgtccgggg actttgtgaa ccgacaccga gaaaaattca tgccagaaca gtgtcatctt
     1021 ttcctgtaac tggcgctgag aataggtcat ccgggcgacc cagcgacggc g
//
LOCUS       DAOQDO010002496         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_190264_length_1070_cov_3.881773, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002496 DAOQDO010000000
VERSION     DAOQDO010002496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190264_length_1070_cov_3.881773"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..973)
                     /locus_tag="PKZ53_28260"
     CDS             complement(68..973)
                     /locus_tag="PKZ53_28260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44408.1"
                     /translation="MNALHTLLTGLIDYAGLFPPAELGMAAAVQNYAAYRQGPFAWCL
                     GRFIVPVSRLREFEAEAGVLLPTEEGILPWGLSALVGPQIESDVETIIDFNQRHAGPA
                     SGLAVIDTIEIKAATVADIERIQRAIPASFTTYVELSLSETLPALIAALSQTGLRAKL
                     RTGGITPELFPTPAEIVRFCSLCVQNQVTFKATAGLHHPIRSSHPLTYAPNSPQGVMH
                     GFFNVFLGAAFIQAGLSPAEVEQVVLDESPANFQVTDTGIIWQGHHLSLDALRQLRDQ
                     VAVSFGSCSFEEPLQDLQTLNLFGE"
BASE COUNT          257 a          277 c          271 g          265 t
ORIGIN      
        1 ttcatccctc atccttcatc cctcatccct ttagaaaccc ggaacccgga acccgctgcc
       61 tcattcttca ttctccaaac aggtttaagg tttgaagatc ctgaagcggt tcttcaaaag
      121 aacacgatcc aaacgaaaca gccacctgat cacgcaactg tctcagcgca tccagactca
      181 ggtgatgtcc ctgccagatt atgcccgtat cagttacttg aaaattggcc ggtgattcat
      241 caagcacgac ttgttcgact tcagccggtg acagaccggc ctggataaag gcagcaccga
      301 gaaacacgtt gaaaaaccca tgcatgacgc cctgtggact gtttggggca taggtcagcg
      361 gatgcgaaga ccgaattggg tgatggagtc cagccgtggc cttgaatgtc acctgatttt
      421 gaacacacag tgaacaaaag cgcacaattt cagccggggt tggaaacagt tctggcgtaa
      481 tccctccggt tcggagtttg gctcgcaaac cagtttgact gagcgctgcg attaacgctg
      541 gaagtgtttc cgaaagagat agctcaacat aggttgtgaa agaagccgga attgcccgct
      601 gtattcgttc gatatccgcc acggttgcag ctttgatttc aattgtatca atcacagcca
      661 acccggatgc aggcccagca tggcgctggt taaaatcaat tatagtctca acatcagact
      721 cgatctgagg tccaaccagt gcgctgagcc cccagggcaa tattccttct tcagtcggaa
      781 gcagtacgcc agcctcagct tcaaactcgc gtaaccggga tacgggcaca ataaagcgtc
      841 ccaggcacca ggcaaatggt ccctgtcggt acgccgcata gttttggacg gcagcggcca
      901 ttccaagttc tgccggtgga aacaacccgg cataatcaat gagtccagtc agtaaagtgt
      961 gaagtgcatt cattggggtt ctgagttccg agtatttggt gcttggtgcc tggtgcttgg
     1021 ttcttggttc ttggttcttg gttcttggtt cttggtgctt ggtgcttggt
//
LOCUS       DAOQDO010002497         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_191041_length_1068_cov_2.622902, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002497 DAOQDO010000000
VERSION     DAOQDO010002497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191041_length_1068_cov_2.622902"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKZ53_28265"
     CDS             complement(<1..277)
                     /locus_tag="PKZ53_28265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44409.1"
                     /translation="MNYLAASFSALSHPFSPKQKFSALAAVLVVGLTLIIQPPVWAQS
                     ENEPGPDDTALAKVNRVYLAYKPVVERRVQVATWDGSFWSASEPVADA"
     gene            634..>1068
                     /locus_tag="PKZ53_28270"
     CDS             634..>1068
                     /locus_tag="PKZ53_28270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ44410.1"
                     /translation="MHTLWKDIRYGFRTLLTRPGFFLAAIVTIGLGIGANTAVFSVIN
                     AVLLRSLPYPEPERLVILTESTGADAKPVAYPNFLDWRRQSQSFEQLTAYTATDFTFS
                     GTEQAERIDGELVSDGYFDLLGVKPGIGRTFSPEETTVRGAHS"
BASE COUNT          249 a          286 c          277 g          256 t
ORIGIN      
        1 gggcatcagc caccggttcg cttgcagacc agaacgagcc atcccaggtg gcaacttgaa
       61 cccggcgttc aaccaccggt ttatacgcca gatacacccg gttgactttg gcaagggcag
      121 tgtcatctgg tccgggttca ttctcggatt gcgcccaaac aggcggctgg atgatcagtg
      181 tcaatccaac caccaggacc gcagccaaag ctgaaaactt ttgttttggg gaaaaagggt
      241 gagaaagggc cgaaaaagag gcggcaaggt aattcatagg tcaaactcca tcgtgttttg
      301 attgtggcgg aagggaaatc agaccagctt gataggtttc cgatcctaac gcctgagttg
      361 cacacaaaag ttttccagga ttccggctgc aaaataaggg catttggtca gccggtcacc
      421 cagtcacccg gtcacccggt cactctgtcc ctggtttgca acaaaagcat cccacaaccg
      481 aacatctctt tacttgcatt ttttgatgag cctcccgcca ggtgggataa agcaagctgg
      541 tcggagtcag gcatagcggt tgcacagtaa cagcggcttt caaaaacacc tcactcctca
      601 aatcaaaatc ccactcgttt tggagattcc gccatgcaca cgctttggaa agatattcgc
      661 tatggctttc gcacgctcct gactcgaccc ggtttttttc tggctgcgat tgtcaccatc
      721 ggccttggaa ttggggctaa caccgcggtt tttagtgtca tcaatgccgt tttacttcgc
      781 tctttaccct atcctgagcc ggaacgactg gtcattttga ctgaatcaac cggggcggat
      841 gccaaaccgg tggcataccc taattttctg gactggcggc ggcaatcaca atcgttcgag
      901 caattgacgg cctacacggc caccgatttt acgttctcgg gtaccgagca ggccgagcgg
      961 attgacggcg aactggtttc agatgggtat tttgatctgc tgggggtaaa acccggtatc
     1021 ggtcggactt tttcgcctga agaaaccacg gtgcgcggtg cccattcg
//
LOCUS       DAOQDO010002498         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_191701_length_1066_cov_3.278932, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002498 DAOQDO010000000
VERSION     DAOQDO010002498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191701_length_1066_cov_3.278932"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..>1066
                     /locus_tag="PKZ53_28275"
     CDS             370..>1066
                     /locus_tag="PKZ53_28275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ44411.1"
                     /translation="MNLHDESNLGSPFDDGNVYDLLFSNFDYGLEYYLQLARSAQGPV
                     LDIACGTGRVMLPCLEAGLDVDGLDLSVALLDRLREKAAVKGLNPTLFEGDMRQFRLD
                     RRYRLAIIPFNAFVHCMNGEEQIAALRCIREHLEPGGMLAFDTFFPGFEMLNLPNQTR
                     VLEMETDHPTPGWKVRVYDTRTLNRIDQIQHSFMEIEELNDAGEVVNVHPSQTQIRWI
                     YKQEMELLLRLSGF"
BASE COUNT          244 a          268 c          273 g          281 t
ORIGIN      
        1 ccaagcccca agccccaagc cccaagcccc aagccccaag ccccaagccc tggttttctc
       61 agccccacct tgacaatcag caccaacttg ccgtattgga taggccacat ctttttaaac
      121 gagctgccta tgattgcctc ttcaaactaa gttgaacctg ttgtgaaacc acatccggtt
      181 tctggccttt gccttaacgg gcggagtgtg tctggctggt tcgacttttt tcgcacacat
      241 tttccctttg tccaattgac cccgaccagt attgctaccg atgtcatgtc aggcgtcgga
      301 ttgcgtgctg tgtgtgtcga aatcccacac ctgacaggtg tggtaaagat ttgaagagga
      361 ttttcagcca tgaatttgca cgatgagtca aatttaggat cgccatttga tgatggcaat
      421 gtgtacgacc ttctgttttc caattttgat tatggcctgg agtattacct gcaactggct
      481 cgatctgccc agggcccggt gcttgatatt gcctgtggta ccgggcgcgt gatgcttccg
      541 tgcctggagg ctggccttga cgtagacgga ctcgatttgt cagtcgcact gcttgaccga
      601 ttgcgagaaa aggcagccgt aaaaggattg aatccaacgc tgtttgaagg agatatgcga
      661 cagttccgcc ttgaccggcg atatcggctg gccattatcc catttaatgc ttttgtgcat
      721 tgtatgaatg gtgaagagca aattgcggcg ttgcggtgca tccgcgaaca cctggagccg
      781 ggcggaatgc tggcatttga taccttcttt ccagggtttg agatgctcaa cctgccaaac
      841 caaaccagag tgttagaaat ggagaccgac catccgacgc cagggtggaa ggttcgagtt
      901 tatgacactc gcacgctcaa tcggatagat cagattcaac attcctttat ggaaatcgag
      961 gaactcaacg atgccggtga agttgtgaat gttcatccga gccagaccca gattcgctgg
     1021 atttacaagc aggaaatgga gcttttgctg cggttgagcg gattct
//
LOCUS       DAOQDO010002499         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_192086_length_1065_cov_2.770297, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002499 DAOQDO010000000
VERSION     DAOQDO010002499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192086_length_1065_cov_2.770297"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKZ53_28280"
     CDS             complement(<1..685)
                     /locus_tag="PKZ53_28280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44412.1"
                     /translation="MPGNVYRQWPTRVVGLLLTGFFLISSGLAQSQDPVLSQVKGVAV
                     VSNLPAMSDVQLREHVRVLTMPVMEGRRAGTTGDRTAATYIAEQFRRAGLVPIGDNNT
                     FFQSFQFESWQPVPVTAMSQLEKGTVKKSYTCEWSNSFTGRDFRPVPRSGQGSVTAPV
                     VFVGFGLSEPELNLDDYAGVDVRDKTVLILLGDGPLPTGRARTESAILTAKKRGARAC
                     LLAYPASGYP"
     gene            complement(837..>1065)
                     /locus_tag="PKZ53_28285"
     CDS             complement(837..>1065)
                     /locus_tag="PKZ53_28285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44413.1"
                     /translation="KFLIRYLTQDVENPTNRILLANGVPVIRYESALTSLDTRIVVGN
                     FGGGRNYDVALIEGKVIRVFLDVLEKISPEE"
BASE COUNT          258 a          305 c          247 g          255 t
ORIGIN      
        1 ctgggtaccc agaggctgga taagccagta aacaggcccg tgcacctcgt tttttggcag
       61 tgagaattgc cgattcagtc cgtgccctgc cggtcggaag tgggccatcc cctaacaaaa
      121 tcagcacggt tttgtcgcgg acatccactc cggcatagtc atccaggttg agttctggct
      181 ctgacaaccc aaatccaaca aaaaccaccg gggcggtgac acttccctgt ccggaacggg
      241 gtactggccg aaaatcacgc ccggtaaaac tattgctcca ttcacaggta tatgattttt
      301 tgactgtccc tttctccagt tgactcatgg cggtcaccgg gaccggctgc cacgattcaa
      361 attgaaaact ttggaagaag gtattgttat caccaatcgg caccaggccc gcccggcgga
      421 attgttcggc aatgtaggta gctgcggttc gatcccccgt ggtgccagcc cgacgccctt
      481 ccatcaccgg catcgtcagg actcgcacgt gttcacgaag ctggacatca ctcatcgccg
      541 gcaggttgct taccaccgcg acacctttca cctggctcag gaccgggtcc tgcgactgag
      601 ccaacccaga agaaattaaa aaaaatccag tcagaagaag tccaaccact ctggttggcc
      661 attgtcggta tacatttcct ggcatgcgaa tctcattttc aggcggggaa tctgaagagg
      721 tcccaacgat accaggattt tccgagaatc tgaagaaatc aggtggcaga attggttttc
      781 attcatccgt catccttcag tcagctctcc gttctcagcc aaaatccctg ggttgactat
      841 tcttctggtg agattttctc caaaacatcc agaaacaccc gaatcacttt gccttcgatt
      901 agcgccacat catagttgcg accacctccg aagtttccaa ccacgattcg agtatcgagg
      961 ctggttaagg cgctttcata ccgaatcacc ggcaccccat tggccaaaag aatccgattg
     1021 gttgggtttt ccacatcctg ggttaagtac cttatcaaaa atttc
//
LOCUS       DAOQDO010002500         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_192171_length_1065_cov_2.159406, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002500 DAOQDO010000000
VERSION     DAOQDO010002500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192171_length_1065_cov_2.159406"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="cas1"
                     /locus_tag="PKZ53_28290"
     CDS             complement(<1..871)
                     /gene="cas1"
                     /locus_tag="PKZ53_28290"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNJ44414.1"
                     /translation="MEIRQNTLYLTTQNSYVSRDHLNLKVEIDHVVRLSVPIHHLESV
                     CLFGVITITPQAAQLCWEHGVPVNYFSENGYLIGRWEGVPNTSVVLRRTQYRTADDPQ
                     KSALIARQCVAGKIQNSRQSLLRSARESSDDQERDQLQLMAGKLADILRWLHPSTDLD
                     TIRGYEGQAASLYFQVFDFHLRQQRTEFHFGGRSRRPPRDRINCLLSYLYALLRHDCI
                     AALTATGLDPFVGYLHAERPNRPSLALDLMEEFRPQVDRLAITLVNRKQVNAKDFVIR
                     DGNSVEFSPAGRKS"
BASE COUNT          249 a          254 c          321 g          241 t
ORIGIN      
        1 ccgatttgcg accggctggc gaaaattcaa cagaatttcc gtcgcggatg acaaagtctt
       61 tggcgttgac ctgcttgcgg ttgacgaggg taattgccag ccgatcaacc tggggacgaa
      121 actcctccat taaatcaagt gccagcgaag gacggttggg acgttcggcg tgcaaatatc
      181 cgacaaaggg atctaacccg gtcgcggtca aagcggcaat gcagtcatgc cggagtaagg
      241 catagagata ggaaagtaag cagttgatgc gatcacgcgg aggccggcgc gaacgccctc
      301 caaaatgaaa ttcggtgcgt tgctggcgca aatgaaaatc aaagacctga aagtacagac
      361 tggcagcctg gccttcatat ccacggatcg tatccagatc ggtggatgga tggagccagc
      421 gcaggatatc ggccagtttg ccggccatga gttggagctg gtcgcgttcc tggtcatcgc
      481 tcgattcacg ggcagagcgc agcaggctct gacgtgaatt ctggatcttt ccggccacac
      541 actgacgggc gatcagagca gatttctgcg gatcgtctgc ggtgcggtat tgggttctgc
      601 gcaacacgac gctggtgttg ggaacgcctt cccagcgacc aatcaaatat ccattttcgc
      661 tgaagtaatt gaccggcacg ccatgttccc agcacagttg cgcggcctgg ggcgtgatgg
      721 taatgactcc gaacaggcag acggattcga ggtggtgaat tggcaccgaa agccgaacga
      781 cgtggtcaat ttcgactttt aaattcaggt ggtcgcgtga cacatagctg ttttgggtgg
      841 tcagatagag cgtattttgt cgaatttcca taatgaggtc ccacgttgag caataccatt
      901 tagggttcag ggttcagggt tcagggttca gggttttcgg aagggatgag agatgaaata
      961 aatcaccttg tcacactgcc agtttgtcat cttgtcatcc tgcccagggt ttcaccgcgg
     1021 agcggtggct gaattgaggc aggtcggcca cagtctgcca ctgtg
//
LOCUS       DAOQDO010002501         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_192667_length_1064_cov_1.625372, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002501 DAOQDO010000000
VERSION     DAOQDO010002501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192667_length_1064_cov_1.625372"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /gene="cas5"
                     /locus_tag="PKZ53_28295"
     CDS             complement(<1..387)
                     /gene="cas5"
                     /locus_tag="PKZ53_28295"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HNJ44415.1"
                     /translation="MTKTFVTIRTWGDFACFTRPEMKVERVSYPVMTPSAARGVLEAI
                     FWEPQMCYLIDSIRVVQRGRWISLRRNEVTARISPAAVQKWIKSPDTFRPIQAGGGAE
                     DGTQRNMLALETVEYIITAEVRLTDQA"
     gene            complement(384..>1064)
                     /locus_tag="PKZ53_28300"
     CDS             complement(384..>1064)
                     /locus_tag="PKZ53_28300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44416.1"
                     /translation="AGVDLDFPLVYRALGPLDSIVQAAGRCNRENRLRDATGMPRLGR
                     VVIFRPAEARLPAGVYKTATDITAITLAQLSSAALATDPTVFERYFTQLYQSTATDVE
                     HSNEPTIQEDREWLRFRSVAEKAKVIRQDPQAVVVAYSDEGRKRIDDLRSRPVTAGQP
                     RFDRTDLRRFQRFMVSLHHHDFQLLQGLGQVHPLLPNLHLFVLSDGLYHDELGVILHQ
                     RPMEDFAL"
BASE COUNT          258 a          293 c          285 g          228 t
ORIGIN      
        1 ggcctggtcg gtcagccgga cctcagccgt gatgatatat tcgactgttt cgagggccag
       61 catattgcgc tgggtgccgt cttcagctcc gcctccggcc tgaatcggac gaaaggtatc
      121 aggtgatttg atccattttt ggactgccgc cgggctaatc cgggcagtga cctcgttgcg
      181 acgaagggaa atccaacgcc ctcgctgtac aactctaatt gaatcaatga gataacacat
      241 ctgaggttcc cagaagatgg cttccagaac gccccgggcc gccgatgggg tcatgaccgg
      301 atagctgacc cgctcaacct tcatttctgg ccgcgtaaaa caggcaaaat caccccaggt
      361 acggatcgtg acaaatgttt tggtcatagc gcaaaatcct ccattgggcg ctggtgaaga
      421 atcacaccca gttcatcgtg atagagtccg tcagaaagaa caaacaggtg caaatttggg
      481 agcaaagggt ggacctggcc caacccttgt agtaactgaa aatcatggtg gtgtaaactt
      541 accataaacc gctggaagcg gcgcaagtcc gtgcgatcaa accggggctg tccggcggtg
      601 acaggtcgtg accgcaaatc gtcaatccgc tttcttcctt cgtcactata agccaccacc
      661 acggcctgtg gatcctggcg aatcactttg gctttctcag ctacggaccg aaaccggagc
      721 cactcccggt cttcctgaat ggttggttca ttggaatgct ccacatcagt ggcggttgat
      781 tgatacagtt gagtgaaata ccgctcaaac accgttggat cagtggccag ggctgcagaa
      841 ctgagctgag ccagcgttat ggctgtgata tccgtggcgg ttttataaac accagccggc
      901 aatctggctt ctgccggtcg aaaaataaca acccggccca gacggggcat tcccgttgca
      961 tcgcgcaacc ggttttcacg attgcaacga ccggcggcct ggacaattga gtcaagtggc
     1021 cccaaggcac gataaaccag cggaaaatca aggtctaccc cagc
//
LOCUS       DAOQDO010002502         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_192833_length_1063_cov_2.732143, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002502 DAOQDO010000000
VERSION     DAOQDO010002502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192833_length_1063_cov_2.732143"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..132
                     /locus_tag="PKZ53_28305"
                     /pseudo
     CDS             1..132
                     /locus_tag="PKZ53_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
     gene            156..1010
                     /locus_tag="PKZ53_28310"
     CDS             156..1010
                     /locus_tag="PKZ53_28310"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44417.1"
                     /translation="MSFSWASDADVRVLGADGPLVITNPIDKTEDRMRPILLPGLVKA
                     VAHNFNYGTDNVRLFEIGKVFEGSLDGFQEAEHLALVVTGAPNTRDWSNQARTETFHT
                     LKGILESILAALQIQGAQFVVPEGDLPQAYFPGQTAQVVVDGKAIGWLGRMSVPATEQ
                     FKLKQPVYLCEVTLGALLHRPQTFAPYRPLPKFPSVERDISALVGTDVSYATIEQTIF
                     GLGLPAFKSMTLKEVFTGKQVPAGKRSLTLNLCYRLENRTLTDQEVSAQHAQVLAALQ
                     SQLGAEIR"
BASE COUNT          240 a          276 c          310 g          237 t
ORIGIN      
        1 aacacggcgg acaacggcgg gttgcgcgtg gcctacgccg cgctccagaa ggcgctcgcc
       61 ggcaagtcgc gcgagccgat cgacgggttc acgccggaac agcggttctt cctcgggttc
      121 gccaatgtct ggttggtcag ggattttccg aggccatttc cttcagttgg gccagcgatg
      181 ctgatgtgcg ggtactgggg gctgacgggc cgctggtgat taccaacccg attgacaaaa
      241 ctgaagatcg aatgcgcccg attttgcttc ctggtttggt caaagccgtg gcgcacaatt
      301 tcaactatgg cactgacaac gtgcgactgt ttgaaattgg gaaggttttt gaaggctcgc
      361 tggatggatt tcaggaagct gaacatctgg ctctggtcgt gaccggtgct cccaataccc
      421 gagactggag caatcaggct cgaactgaga ccttccacac gctcaaagga attctggaaa
      481 gtattctggc ggcactccag attcaaggtg cacagtttgt cgttccggaa ggtgatcttc
      541 cgcaagccta tttcccaggt cagaccgcgc aggtcgtggt ggatggaaaa gcgattggct
      601 ggcttggccg gatgagtgtg ccggctacgg aacagttcaa gctcaaacag ccagtgtacc
      661 tgtgtgaagt cacactgggg gcgctcctgc accgtccgca aacgtttgcg ccgtatcggc
      721 cactgccgaa attcccaagc gtcgaacgcg acatttcagc cctggttgga acagatgtgt
      781 cctatgcgac cattgagcag accatttttg gcctggggtt gccggcgttc aaatccatga
      841 ctttgaaaga ggtgtttacc gggaaacagg ttccggcagg gaagcgttcg ctgacgttga
      901 atttatgtta ccgattggaa aatcgcacct tgacagatca ggaagtaagc gcgcagcatg
      961 cgcaggtact ggccgcctta caaagccaac ttggtgctga aatccgctaa aaatcccata
     1021 aaactgcaaa aaacgcatct cgaatcccat tcgaagcgcg gtt
//
LOCUS       DAOQDO010002503         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_192853_length_1063_cov_2.594246, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002503 DAOQDO010000000
VERSION     DAOQDO010002503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192853_length_1063_cov_2.594246"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKZ53_28315"
     CDS             <1..664
                     /locus_tag="PKZ53_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HNJ44418.1"
                     /translation="TERYIDQVKDWPKSGRHILAHYDDSTIIVYQAYRPSIGNYALRN
                     GHFGGDFSYSRMSWIKPNFLWMMYRSGWGTKQGQEVILGLRLRREFFDQILAQSVQSS
                     YYEGHFATRELWQEALAQSSVRLQWDPDHHPSGAKLERRAVQLGLRGPVLAAFGEKEL
                     IEVIDMYEFVATQRPLTTAEMLKELQTPVERVYIPSDPQIGHRLGLSIAKPGFVEIDE
                     QN"
     gene            700..>1063
                     /locus_tag="PKZ53_28320"
     CDS             700..>1063
                     /locus_tag="PKZ53_28320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44419.1"
                     /translation="MRFVFVLSLLALVAFSHPISAQKARKKSAPSQPQSSEFLTRRLP
                     TGVMTGTFNSFASLLYAVDETILHTDSKDVASTELHSVFPEFYKPTYKEFFDTIARQT
                     KSTWQPDPAHKQWVFSPPA"
BASE COUNT          268 a          267 c          256 g          272 t
ORIGIN      
        1 aactgaacgc tacattgatc aagtcaagga ttggcccaaa agcggtcgtc atatcctggc
       61 gcattatgat gactcaacca ttatcgttta tcaggcatat cgcccatcca ttggcaatta
      121 tgcgcttcga aacggacact ttggcggaga cttttcctac tcgcggatga gttggatcaa
      181 acccaatttc ctctggatga tgtaccgaag cggctgggga accaaacaag gtcaggaagt
      241 tatccttggg ttgcggctcc ggcgtgagtt ctttgatcaa atactggctc agtcggtgca
      301 atcttcttat tatgaaggcc acttcgcaac tcgtgagttg tggcaggaag cgctggccca
      361 atcctctgtc cggttgcaat gggatccaga ccatcatccg tcaggggcca agctcgaacg
      421 gcgagccgtt caacttggat tgcgcggtcc ggtcctggca gcttttggag aaaaagaact
      481 gatcgaagtc attgatatgt atgagtttgt ggcaacccag cgtccgttga ccacagccga
      541 aatgctgaaa gagcttcaaa ctccggttga acgggtgtac attccatctg atcctcaaat
      601 cggccaccga ttgggacttt caatagcgaa acctggtttc gttgaaattg acgaacagaa
      661 ttaaagctgg tgtgcattgc cagtttctga ggtcactgta tgagatttgt cttcgtgttg
      721 agtctgttgg ctctcgttgc attttcgcac ccgatttcgg cccaaaaagc tcgcaaaaaa
      781 tcagcaccgt cacagccaca atcatctgag tttttgacca gacgattgcc aacgggtgtg
      841 atgaccggaa cattcaattc cttcgccagc ctgctctatg cggtagatga gacaattctc
      901 cacactgact caaaagatgt ggccagcact gaacttcatt cagtgtttcc agaattttac
      961 aagccaacat acaaggaatt tttcgacacg attgcccgcc aaactaaatc aacctggcag
     1021 cctgacccgg cacataagca gtgggttttt tcgccaccgg cag
//
LOCUS       DAOQDO010002504         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_193507_length_1061_cov_3.624254, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002504 DAOQDO010000000
VERSION     DAOQDO010002504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193507_length_1061_cov_3.624254"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="fahA"
                     /locus_tag="PKZ53_28325"
     CDS             complement(<1..955)
                     /gene="fahA"
                     /locus_tag="PKZ53_28325"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNJ44420.1"
                     /translation="MTYHINETHNPDLRSWVKSANTPGSDFPIQNLPFGVFKRPHSQE
                     SPSVGVAIGDSILDVNRCLMLGRLTDLAAEAANACRNPRLNQLLALGPEAWSALRQQI
                     SQVLRDDFEASEVCEQLEKACLVNMAEVQMVLPAQIGDYTDFYASVYHATNVGSMFRP
                     DNPLLPNYKHIPIGYHGRASSIFPSGTPVRRPKGQTTEGNSDVPTFGPCRLLDYELEV
                     GFLVGPGNESGTSISIQEAERHIFGVCLVNDWSARDIQKWEYQPLGPFLAKSFATTIT
                     PWIVTLEALAPFRIPAFSRPESDPTPLEYLMSEADQSHGGIN"
BASE COUNT          269 a          252 c          293 g          247 t
ORIGIN      
        1 ggttaattcc gccgtgactc tgatcggcct cactcatcag gtactcaagc ggtgtcgggt
       61 cggattccgg acgtgaaaaa gctggaatcc gaaacggggc cagggcttca agtgtcacaa
      121 tccagggtgt gatcgtggtc gcaaaactct tggccagaaa tggaccgagt ggctggtatt
      181 cccatttttg aatgtcacgc gccgaccagt cattgaccag acagactcca aaaatatggc
      241 gctctgcttc ctggattgaa attgacgtcc ccgattcatt tccgggacca accaggaagc
      301 caacttccag ttcataatcc agcaaccggc aggggccaaa cgtcgggacg tccgaattgc
      361 cttccgtcgt ctggccttta ggacggcgaa ccggtgttcc actgggaaag attgatgaag
      421 cgcgaccgtg atagccaatc ggaatatgtt tgtaattggg caataaggga ttatcaggcc
      481 gaaacatact gccgacattg gtggcgtgat acaccgaggc ataaaagtcg gtgtaatctc
      541 cgatctgggc cggcaacacc atttgaactt cggccatatt taccagacag gctttttcta
      601 actgttcaca gacctcggaa gcttcaaaat catcgcgcag cacctgactg atttgctggc
      661 gcagagctga ccaggcttct ggtcccaatg ccaaaagctg gttgagccgt ggattgcgac
      721 aggcattggc ggcttcagca gctaaatcgg tcaagcgccc cagcattaaa cagcgattga
      781 catccaaaat tgagtcccca atggcaactc cgacgctggg tgattcctgg ctgtgtggac
      841 gcttaaagac accaaacggt aaattctgaa tcggaaagtc tgaaccaggg gtgtttgctg
      901 atttgaccca ggaacgcaag tccgggttgt gggtttcatt gatgtggtag gtcataacgg
      961 aggaaaacag aatgaagaat gaagaattga gaatgaagaa tgaagaaaat acgggtgtct
     1021 ggtgcctggt tcttggtgcc tggttcttgg tgcttggtgc t
//
LOCUS       DAOQDO010002505         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_194591_length_1059_cov_1.221116, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002505 DAOQDO010000000
VERSION     DAOQDO010002505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194591_length_1059_cov_1.221116"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1059)
                     /locus_tag="PKZ53_28330"
     CDS             complement(89..>1059)
                     /locus_tag="PKZ53_28330"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNJ44421.1"
                     /translation="FLIDSYLLQSFTFDKVQTYFGTEVEKPFTLSVTRDGRKVRGVPR
                     AYDVMAHLGSPEALKQLLLDGDLDYTGYRNQLKRLDQERLQLRAPATFYNRYLSAVSE
                     NLMGTNYGNGVPNLRLTSSVGAWASLRHDVVAYAKQSYTSVGRGLAMSPPLPSRVPVM
                     RVAPAAPVFRHLGQAIRLILPTFENESSHRQLLAGQQLLETLDLLERTAGTGPLTETD
                     AMQLAKAIRDWAGGPPQTLIADVHTDANSNEVLEIGVGQSATLSFEDGDQDQPSKGVV
                     FSVYEFRWPISQRLTDEAWREMLKQKAGSLRKLTFFLSPPKSTNNP"
BASE COUNT          250 a          266 c          285 g          258 t
ORIGIN      
        1 gatgataaaa ttatcgcggt gatgaagggt gatgaggtgt atcagaattg gaaaaactcc
       61 attctgtagt tgaggcagat tcttcctctc acgggttgtt ggtgctcttt ggaggtgaca
      121 agaagaaggt taatttccga aggcttccag ctttctgttt gagcatctca cgccaggctt
      181 cgtcggtcaa tcgctgtgag attggccatc gaaactcata gactgaaaac acaacccctt
      241 tcgagggttg gtcttgatcg ccatcctcaa acgagagggt ggcgctttgg ccaacgccga
      301 tttccaggac ttcgtttgag ttggcatccg tgtggacatc ggcgatcaga gtttgcggcg
      361 gcccacccgc ccaatctcga attgctttgg ccagttgcat ggcatcagtc tcagtgagcg
      421 gtccggtgcc ggcagttcgc tccagcaaat cgagcgtttc caaaagttgt tgaccggcaa
      481 gcaattgccg atgcgagctt tcattttcaa acgtcgggag gatcagtcga atggcctgcc
      541 ccagatgccg aaacacaggt gcggctggag ccactcgcat aaccggtacc ctggatggca
      601 gcggtgggct catggcaagc cctcgtccca cactggtgta actttgtttg gcatatgcca
      661 ccacatcgtg gcggagagat gcccaggcac caactgaaga ggtcaaccgc agattgggga
      721 caccattccc ataattggtt cccatcagat tttcggaaac ggcacttaaa taccgattgt
      781 aaaatgtcgc aggggcacgc aactgtaacc gttcttgatc aagtcgtttc agttggttcc
      841 gatagccggt gtaatcgaga tctccatcca gcaagagttg cttgagggct tcaggtgaac
      901 ccaggtgagc catcacatca taggcccggg gaacccctcg aactttgcgg ccatcccggg
      961 ttaccgacag ggtaaacggc ttctccactt cggttccaaa gtaggtttgt actttgtcaa
     1021 acgtaaatga ttgaagcaga tacgagtcta ttaaaaacc
//
LOCUS       DAOQDO010002506         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_195351_length_1056_cov_3.321678, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002506 DAOQDO010000000
VERSION     DAOQDO010002506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195351_length_1056_cov_3.321678"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..826
                     /gene="bioD"
                     /locus_tag="PKZ53_28335"
     CDS             95..826
                     /gene="bioD"
                     /locus_tag="PKZ53_28335"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNJ44422.1"
                     /translation="MTHYRGKGIFITGTDTGIGKTTVGAALVAALVATNQTVRVVKPV
                     ESGCLPDKEGNLMPADALELRHASSFPGCPLAEVNHYALAEPLAPAVAAERAGVELDL
                     AKCVNLVRQAQATADWVVVEGAGGLLVPLAGNRQSGYITVADFIQLLGFPSLVIARTR
                     LGTINHTLLTLNELERRNMSCLGVILNQSDPQKGLEDEDNVHVLQALGVRVLATVPFI
                     EQFTSEEQIQYLQPVLDSLERSYQL"
BASE COUNT          230 a          276 c          291 g          259 t
ORIGIN      
        1 cttaccaaat caatggccac aaagcaaaac tgaatccatt tggagtgcgg cccacttgac
       61 gccgctttga tttcctccag cagaaaaact gaacatgaca cattatcgcg gaaaaggaat
      121 ttttatcacc ggtactgata ccgggatcgg aaaaaccaca gttggtgcgg cgctggtcgc
      181 ggcactggtc gcaaccaatc aaactgtgcg agtggtaaaa cccgtggaat cagggtgttt
      241 accagataag gaagggaatt tgatgccagc agatgcgctc gaactcaggc atgcttcaag
      301 ttttcccggg tgcccgttgg ctgaggtcaa tcactacgcg ctggctgagc cattggcccc
      361 agcggttgct gccgaacgcg ctggtgtcga actcgacctg gccaaatgtg tcaatctggt
      421 tcggcaggcc caggcaacgg ctgattgggt ggtggtcgag ggcgccggcg gattgctcgt
      481 accgctggct gggaatcgtc aatctggcta catcaccgtg gcggatttta ttcaactgct
      541 gggattccca tcgctggtca tcgcccgtac ccgattgggc accatcaacc ataccctgtt
      601 gacactcaat gagttagaac gccgaaacat gagctgcctg ggtgtgattc tcaaccaatc
      661 ggacccgcaa aaagggcttg aagacgaaga caatgtccac gtgcttcaag ccctgggtgt
      721 tcgggtgctg gcgacggtgc cgtttatcga acagtttact tcagaagagc agatccaata
      781 tttgcagccc gtcctggaca gtttagagcg atcttaccaa ctctgaatgc tgactgactg
      841 gaatcgtgag tttcgcgcca caatgtcagt tgctcgctcc ttcgttcact tcaccccttc
      901 gctcatttcg atggttggtg ccggggcggt ttgagggatt ccagccggca tttgttttcc
      961 agtttgctgg ttgcggcggg cttcggcttc ggcgcgtttc ttctgatttg gaatgtattc
     1021 tttccagaca aagtctttga gggctgcgac cacaac
//
LOCUS       DAOQDO010002507         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_195396_length_1056_cov_2.609391, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002507 DAOQDO010000000
VERSION     DAOQDO010002507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195396_length_1056_cov_2.609391"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1056
                     /gene="pglZ"
                     /locus_tag="PKZ53_28340"
     CDS             <1..>1056
                     /gene="pglZ"
                     /locus_tag="PKZ53_28340"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HNJ44423.1"
                     /translation="QNRLDQILRELQIDSLAHLSQFSELGFEQSLGMYAEALKQVLRD
                     RTSTQLESLMSAEAFLKRYQLPQSSTARFERVRMASRLARWLVDQPGGKLGTLAEAMA
                     AYTETSCFVDWARTVVGAGDPHPELSRALTWLLEVVTEIREPENQTFARFLVDWTEAG
                     SPECGVLLIEQLLSRVVAPLAQHVPVLLIVMDGMSFSVFRELSQDLDKTGWNRVVPST
                     HPYSGMALAGLPTVTQTSRASLFCGQLVEGDKDLETRGFRDHPDLKKNSRVQLPPKLL
                     HKADLDLDFREMIINPKQKIIGVVVNVIDDLLSSGSQLFFPWALHRIPVLEQLLAAAQ
                     DGERVVILTSDHGHVLEA"
BASE COUNT          238 a          246 c          302 g          270 t
ORIGIN      
        1 ccaaaatcgg ctcgatcaaa ttctgagaga acttcagatt gattcgttgg cacatctgag
       61 ccagttttca gagcttgggt ttgagcaatc gcttggaatg tatgccgaag cactcaagca
      121 ggttcttcgt gaccgaacca gcactcagtt ggaatcactg atgtctgccg aagcctttct
      181 gaaacggtat cagcttccac aatccagcac tgctcgattt gagcgggtcc gtatggcttc
      241 gcggctggcc cgatggctgg tggatcagcc tggagggaaa ctcggtaccc tggctgaggc
      301 gatggcggcc tatactgaga cttcgtgttt tgttgattgg gcgagaacgg tggttggggc
      361 aggggatccg cacccggagt tgagccgggc attaacctgg ttactggaag tcgtgactga
      421 gattcgcgag ccggaaaacc agacctttgc tcgattcctg gttgattgga ctgaagccgg
      481 gtcaccagaa tgtggcgtgc tgctgattga acagctgctt tcgcgggtgg tggcaccgct
      541 ggcccagcac gtccccgttt tgttgattgt catggacggg atgagtttca gtgtttttcg
      601 tgagttaagc caggacctgg ataaaaccgg atggaaccgg gtggtgccat ccactcatcc
      661 atattcgggc atggccttag ccgggttgcc gaccgtgact caaacttcac gggcaagttt
      721 attctgtggg caacttgttg aaggtgacaa agaccttgaa accagaggat ttcgcgatca
      781 cccggattta aagaaaaact cacgcgtcca attgccacca aagttgttgc acaaagccga
      841 tctggatttg gatttccgag aaatgattat aaatccaaaa caaaaaatta tcggggtcgt
      901 ggtcaatgtg attgatgatt tgctttcaag tggcagccag ttatttttcc cctgggcatt
      961 gcaccgcatt ccagttttgg agcaactcct ggctgcggca caggatggcg aacgggttgt
     1021 tatcttgacc tcagaccacg ggcatgtttt ggaagc
//
LOCUS       DAOQDO010002508         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_195483_length_1056_cov_2.098901, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002508 DAOQDO010000000
VERSION     DAOQDO010002508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195483_length_1056_cov_2.098901"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKZ53_28345"
     CDS             complement(<1..920)
                     /locus_tag="PKZ53_28345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ44424.1"
                     /translation="MRPKFRNSFGVLLCGCLIFAVTFSGCDIFRPVRKLAEGIEAKSM
                     SCEPGSQGGTLTLALQNGPTTFNPYVDASPDTYEVMRNLYTTLLDYDYAKNKVEDTGL
                     ATTVTPGADGQSFSIKVRKALFSDGSSLDAEDVVFSYQTCVSSEMDSLLGDLLRTRQD
                     KVVKEPTITKVDGQTLTVKFGGPISPDVARLIFARIPIVSKNSFGAALKSGSARDVYG
                     LKTAPDKIVTSGPYKVASCSASELVLKANEKYYKTDSNGTLLPYLDEVKYMLNLDRKA
                     QSDKFAAGALDACNYLTAEKLKSLKGNNQFL"
BASE COUNT          279 a          258 c          270 g          249 t
ORIGIN      
        1 agaaactgat tattcccttt gaggcttttc aacttctcag ccgtcaaata gttgcacgca
       61 tccagcgcac ccgccgcaaa tttatcggat tgggctttgc gatccaggtt gagcatgtac
      121 ttgacttcgt ccagataggg caggagtgtg ccgttggagt ccgtcttata gtacttttca
      181 ttggctttca ggacgagttc acttgctgaa caggaggcca ctttataggg acctgacgtg
      241 acgattttat caggggcggt ttttagacca tagacatctc tggcactgcc gcttttgagt
      301 gcggccccaa aactgttttt ggaaacaatc gggattcggg caaaaatcaa acgggcgaca
      361 tcaggtgaaa tcggcccacc aaacttaacc gtcagcgtct gaccgtcaac cttggtaatc
      421 gtgggctctt tcacgacttt atcctggcgg gttctgagca aatcacccag caacgagtcc
      481 atttcggaac tgacacaggt ttgataggaa aaaaccacat cttcagcatc gagcgaagag
      541 ccatcagaga acaaggcttt gcggactttg atcgaaaagc tctgcccatc agcgccaggt
      601 gtcaccgtgg tggccagtcc agtgtcttcg actttgtttt tggcatagtc atagtcgagc
      661 agggtggtgt acaggttgcg catgacctca taggtatcag gcgaagcatc cacatacggg
      721 ttaaacgtcg tcggcccgtt ttgcaaggcc agggtgagag tgccgccctg ggaaccaggc
      781 tcacagctca tagatttggc ttcaatgccc tctgccagtt tacgaacggg tctaaaaatg
      841 tcacacccag aaaaagtaac agcaaaaatc aggcagccac aaagcagcac cccaaacgag
      901 ttgcggaatt ttggtctcat tcgaaaactc ctccaggaag gtatcgtgca cggttcgagg
      961 aaaaaaataa cgagtggaaa cggcaacaag gcgacaggac gaaaaaaatc tgaagtgagg
     1021 ctggatttta ctgaatttcc cggctacagg tctagc
//
LOCUS       DAOQDO010002509         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_195763_length_1055_cov_3.023000, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002509 DAOQDO010000000
VERSION     DAOQDO010002509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195763_length_1055_cov_3.023000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1055)
                     /locus_tag="PKZ53_28350"
     CDS             complement(<1..>1055)
                     /locus_tag="PKZ53_28350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ44425.1"
                     /translation="SPDNEIIRKVGRSFSQFDYLVSPNQTLTATINFSKGVYKHIGLD
                     FFTPQTAAPDRRPTDLTVALIDRLTLSNGSLLETIFSYKRLDTRIEGKGLDPLVLTPS
                     VREGNYFHFDDRLTERYQLRSTNEFAPITAAGVHRVKLGADFNYTRNNSLIMNQPVSV
                     TRMDGTLAQLIEFTRPGNLRSYNFELAAFAQDQWMVSPKLSLDYGFRAEAVRATAGIN
                     ISPRVSLSYSPTESGNTVFRAGVGLFYDKIPLNALTFTMSPMQTVTTYAADGTTVVDS
                     SRFFQNVLAARPNGKPNSGVDFAAPRNVTYNLEFNRRVTQNILLKLAYLDSKTDNDLY
                     VSPVTTETGNQIVLNNN"
BASE COUNT          260 a          256 c          288 g          251 t
ORIGIN      
        1 gttgttattg agcacaatct ggttgccggt ttcagtggtc acgggcgaaa catagaggtc
       61 gttgtcggtc ttgctatcca ggtaagccag tttcaacaga atgttttggg tcactcgccg
      121 gttgaattcc aggttgtagg tcacattgcg cggtgccgca aaatcaactc cactgttggg
      181 ttttccattt ggacgggctg ccagcacgtt ttggaagaac cgggatgaat caaccacggt
      241 tgtgccatcg gcggcataag tggtcaccgt ctgcattggc gacatggtaa aagtcagagc
      301 attaagcggg atcttgtcat aaaacaaccc aactccggca cgaaagacag tatttccgct
      361 ttcagttggc gagtaggaga gtgaaacccg aggtgaaata ttgatccccg ccgtcgcccg
      421 aaccgcttca gcccggaacc catagtccag gctcagtttg gggctcacca tccattgatc
      481 ctgggcaaag gccgccagtt caaaattgta ggaccgtaaa ttcccaggtc gtgtaaattc
      541 aatcaactgc gccagcgtgc catccatgcg ggtgaccgaa accggctggt tcatgatcag
      601 cgaattattc cgcgtgtaat tgaaatctgc gccaagtttg acccggtgga cacccgcggc
      661 ggtaatcgga gcaaattcat tggtggatcg aagttgataa cgctccgtta accgatcatc
      721 aaagtggaag taatttcctt cacgaacact tggagtcaaa accagtgggt caagcccttt
      781 gccttcaatc cgtgtgtcaa gccgcttata tgaaaaaatg gtttccagga gcgatccatt
      841 tgacagggtc agccgatcaa tcagggccac cgtcaaatct gttgggcgac gatcaggcgc
      901 ggcggtttgc ggcgtgaaga aatccagccc gatgtgctta tagacaccct ttgaaaagtt
      961 gatggtggcc gtcagggtct ggttcgggga aaccaggtaa tcaaactggg aaaaactccg
     1021 gccaactttg cgaatgattt cgttgtcagg cgagg
//
LOCUS       DAOQDO010002510         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_195794_length_1055_cov_2.679000, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002510 DAOQDO010000000
VERSION     DAOQDO010002510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195794_length_1055_cov_2.679000"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PKZ53_28355"
     CDS             complement(<1..1050)
                     /locus_tag="PKZ53_28355"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNJ44426.1"
                     /translation="MLQNKGTISLITEDLDLFLSPAPDMKEELRPAREKAAKALEAYG
                     TWLEKELLPRSNGDFRIGDKNFRTKLRYTLESNLTKEDILKRCETELKQTQETMYATA
                     LPLFKKAFPEVTDAKKLEDRKYIIKSVLDTLAEDRPNNETIVPLATKQLTECTEFVRT
                     QQLMTVPDEPVKIIEMPEYQRGVAVAYCDSPGALEKNAETFFTISPTPADWPATRVTS
                     YFKEYNNYMTQNLTIHEAMPGHYLQLMHANRFKAPTMVRAIFSSGTFVEGWATYAEQL
                     MAEKGFGGPSVKMQQLKMRLRLIINAMIDQKIHTAGMTEKEAMDLMMNEGFQEEGEAA
                     GKWRRACLSSCQLSTY"
BASE COUNT          225 a          265 c          255 g          310 t
ORIGIN      
        1 ataggttgat aactggcacg atgacaaaca ggcccggcgc catttcccgg cggcttcccc
       61 ttcctcctga aagccttcat tcatcatcaa gtccatcgct tctttttcag tcatccctgc
      121 cgtgtgaatt ttctgatcaa tcatggcatt gatgatgagc cgcaaccgca ttttgagttg
      181 ctgcattttg accgatggcc cgccaaatcc tttttcagcc atcaattgct cggcataggt
      241 agcccaacct tcaacaaacg tgccacttga aaaaatggcc cggaccatcg tcggtgcttt
      301 aaaccggttg gcgtgcatca attgcagata atgccccggc attgcttcgt gaatggtcag
      361 gttttgggtc atgtaattgt tgtattcttt gaaatacgag gtgacccgcg tggccggcca
      421 gtcagcgggt gtcggagaaa tggtaaaaaa cgtctcggca tttttctcca gcgcacccgg
      481 cgagtcacaa taagccacag cgacgccacg ctgatactcg ggcatttcga tgattttgac
      541 cggctcatct ggaactgtca tcagttgttg ggttcgcaca aattcagtgc actctgtgag
      601 ctgtttggtc gccagcggta caatcgtttc gttattgggg cgatcttcag cgagcgtatc
      661 cagcactgac ttgatgatat atttgcgatc ttcaagcttt ttggcgtcgg tcacctcagg
      721 aaacgccttt ttgaaaagcg gcaaggccgt ggcgtacatc gtttcctgag tttgttttaa
      781 ttcggtttcg catcgtttca gaatgtcttc tttggtgaga tttgattcaa gtgtataccg
      841 gagttttgtc cggaaattct tgtcaccgat tcgaaaatca ccgtttgacc ttggcagcaa
      901 ctccttttca agccaggtac catatgcctc aagggctttg gccgcttttt ccctggctgg
      961 ccgcagttcc tctttcatgt ccggtgctgg cgagagaaac aaatccagat cttcagtgat
     1021 caaactgatt gtgcctttgt tctgtaaaat ggcgg
//
LOCUS       DAOQDO010002511         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_196103_length_1054_cov_3.596597, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002511 DAOQDO010000000
VERSION     DAOQDO010002511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196103_length_1054_cov_3.596597"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKZ53_28360"
     CDS             complement(<1..976)
                     /locus_tag="PKZ53_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNJ44427.1"
                     /translation="MSDVKSSKFTNRLINETSPYLLQHAHNPVDWYPWGPDALARAKA
                     ENKPILLSIGYSACHWCHVMEHESFENEAIAKLMNEHFINIKVDREERPDLDTIYMNA
                     VQMLTGRGGWPMTVFLTPDQKPFFGGTYYPPQDRYGMPGFPRVLLSVSDAYRTRLDDI
                     LGQAQEILAELDKMQDLKPSAAPLEKSILSAAATQLKKRFDWTHGGFGQAPKFPNSMA
                     MSFLLRYWHDTADPQSLEMIEQSLLKMAQGGIYDQLGGGFHRYSTDARWLVPHFEKML
                     YDNALLSQLYLEVHLATGEDIYRQIVEETLDYVVREMTSPEGGFYSTQD"
BASE COUNT          256 a          280 c          268 g          250 t
ORIGIN      
        1 cgtcctgggt cgagtaaaaa ccaccttccg gtgatgtcat ttcacgaaca acataatcga
       61 gcgtctcttc aacaatctgg cgatagatgt cttcgcccgt ggccagatgc acttcgaggt
      121 acaactggct gagtagcgca ttgtcataga gcatcttctc aaagtgcggc accagccacc
      181 gggcatcggt tgaatagcga tgaaacccgc cgccaagctg gtcataaatc ccaccctgcg
      241 ccattttcag cagtgactgt tcaatcatct caagtgactg tgggtcggcg gtatcgtgcc
      301 agtagcgcaa caaaaacgac atggccattg agttcggaaa ctttggggcc tggccaaacc
      361 caccgtgagt ccaatcaaag cgcttcttta attgcgtggc ggcagcactc aaaatggatt
      421 tttcaagcgg cgcggctgac ggcttgagat cctgcatttt gtcaagttcg gccagaatct
      481 cctgagcctg acccaggatg tcgtccagac gggttcgata ggcatctgaa actgaaagca
      541 gcacccgtgg aaatccaggc atgccatagc ggtcctgggg tggatagtag gtgccaccaa
      601 aaaatggctt ctgatccggc gtcaaaaata ccgtcatcgg ccaaccacca cgcccggtaa
      661 gcatctgaac ggcattcata taaattgtat cgagatcggg ccgctcttca cggtccactt
      721 taatattgat gaagtgctca ttcatcaatt tcgcaatcgc ctcattttca aacgactcgt
      781 gctccatgac atggcaccag tgacaggctg aatacccgat gctgagtaaa atcggtttgt
      841 tttcagcctt tgcgcgcgcc agggcgtcgg ggccccacgg ataccagtcc acggggttat
      901 gggcatgttg taataaataa ggactggtct cgttgatcag ccggttggta aatttcgatg
      961 acttgacatc gctcatgcaa ctcagttcct ttggtcaacc aaactcgttt gcgccgaaca
     1021 gcgcggggtt tcttgggtac agggtcgaaa ccat
//
LOCUS       DAOQDO010002512         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_196515_length_1053_cov_2.810621, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002512 DAOQDO010000000
VERSION     DAOQDO010002512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196515_length_1053_cov_2.810621"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..880
                     /locus_tag="PKZ53_28365"
     CDS             101..880
                     /locus_tag="PKZ53_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ44428.1"
                     /translation="MRIRLAVIAMPDLRSTINLNELDIYVELSGTGVPLVLLHGFSGA
                     GCNWELIFPEPPAGFQLIVPDLRGHGRSTNPSGIFTHRQSALDVFALLDQLGIMTFRA
                     IGMSCGAKTLLHMATQQPDRVEAMVLVSATPYFPKEARLAMSQLSPDNRSEAEWEQMR
                     RWHKHGDNQIRLLWTLGNRFKDSYDDVNFTPPLLSTIKARTLIVHGDRDPLYPVALAL
                     EMYQAIPKGMLWVVPNGGHGPIFGDQTPHFVETAVKFLMTQ"
BASE COUNT          244 a          278 c          276 g          255 t
ORIGIN      
        1 agcccacgac cacgccaaac cagttgctgg gggacattga aatctgctta ctgttctgaa
       61 taaatggggt tactggccgc accgctcttg atttcccatt ttgcggattc ggctggctgt
      121 gattgcgatg ccggacttgc gaagtacaat aaacctcaac gagttggaca tatatgttga
      181 actctctgga actggagtgc cgctggtgct cctccacggg tttagcggtg ccgggtgtaa
      241 ctgggaattg atttttccgg aaccaccagc cgggtttcag ttgattgttc ccgatttgcg
      301 cgggcacgga cgatcaacca acccctcagg catatttacc catcgtcaat cggcgctcga
      361 tgtctttgct ctgcttgatc agcttggaat catgaccttt cgagcaattg gaatgagttg
      421 cggcgccaaa accctgctcc atatggctac ccaacaacct gatcgggtag aagcaatggt
      481 gctcgtcagt gcgactccct atttccccaa agaagcccgc ctggccatga gccagttgtc
      541 tcccgacaac cgaagtgagg ccgaatggga gcaaatgcga cgatggcaca aacacggaga
      601 taaccagatt cggttattgt ggacgcttgg gaaccggttt aaggatagct atgacgacgt
      661 gaactttacg ccgccgttgc tttcaaccat caaagcccga acactgattg tccacggaga
      721 ccgcgaccca ctgtatccgg tggcgctggc ccttgaaatg tatcaggcaa ttcccaaggg
      781 gatgctgtgg gtcgtcccga atggcggtca tggtccgatt tttggcgacc agacaccaca
      841 ttttgtggaa accgctgtca agttcttgat gactcagtga tccttcaggc gggagagagc
      901 ctcccttttc tggaaatcct ttttgacaag aggcttattg gcaaggtatg gtccagccag
      961 gctgatcagg gacgcctgca cccgcaaaag aggacctcac ccgttgaagt tgtagatgtg
     1021 caacaagttc ataaaacttc tttgccccaa atc
//
LOCUS       DAOQDO010002513         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_196919_length_1052_cov_3.015045, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002513 DAOQDO010000000
VERSION     DAOQDO010002513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196919_length_1052_cov_3.015045"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKZ53_28370"
     CDS             <1..741
                     /locus_tag="PKZ53_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ44429.1"
                     /translation="EKGSFTGAYAEKKGLFEMADKGTLFLDEIGELNVNLQAKLLRAL
                     QEGEIQRVGATKTIKIDVRVVTATNRDLEAMVKDGKFREDLYYRLNVIPVEVPPLRSR
                     RGDILVLAEYFLKKQIRGSQPAFKFSPEALRLLQNYTWPGNVRQLESAIKRAVLLCEG
                     DRIEVEDLPLEIRQATDRSVEFHFKLPPEGISFEEVERSLIIQAMDQTDWNITRAAKL
                     LGLTFRTLQYRLEKFNLRRPGRGDEDEE"
     gene            complement(801..>1052)
                     /locus_tag="PKZ53_28375"
     CDS             complement(801..>1052)
                     /locus_tag="PKZ53_28375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44430.1"
                     /translation="GMVSQRIDKFDTRPFSCVWEGQRQQSLGLFFSSQGRKNRGQTGT
                     VTTILNVHSKLSRAKKRKHNREKAFFRDGDHGSSSLKDE"
BASE COUNT          253 a          242 c          286 g          271 t
ORIGIN      
        1 gaaaaagggt cgtttaccgg tgcctatgcc gaaaagaagg gcctgtttga aatggccgac
       61 aaaggcacgc tctttctgga tgaaattggc gaactcaacg tgaatttaca ggccaaactt
      121 ctgcgggcgc tccaggaagg tgaaatccaa cgggtgggtg ccaccaaaac catcaaaatt
      181 gatgttcggg tggtcacggc aaccaaccgc gatttggagg ccatggtcaa ggatgggaag
      241 tttcgggaag atttgtatta tcgcttaaat gtgatccctg ttgaagttcc accgttgcgg
      301 tcccgccggg gggacattct ggtgctggct gaatattttc tgaaaaagca gattcgtgga
      361 agccagccgg cctttaagtt ttccccggaa gccttacggc tgctccaaaa ttacacgtgg
      421 cctggtaatg tccgccagct tgaatcagcc atcaaacggg cggtgttatt gtgtgaagga
      481 gaccggatcg aggttgaaga tttgccgctc gaaatccggc aggcaactga ccgctcagtg
      541 gagtttcatt ttaaattgcc gccagaaggt atttcgtttg aagaagttga acgctcattg
      601 attatccagg ccatggatca aaccgattgg aatatcaccc gtgccgcaaa gctccttggg
      661 ctgacctttc ggacactcca ataccggctg gaaaaattta acctcaggcg cccaggtcgg
      721 ggtgatgaag acgaggagtg agcgtaccca ggttcggttg agatccagtt tctatctcga
      781 agttgctggt tggtgtgagt tcactcatct ttgagtgaag atgagccgtg gtctccatcc
      841 cggaaaaagg ctttttcacg gttgtgtttt ctttttttag cgcgcgataa cttgctgtgg
      901 acgttgagta tagtggtgac agtaccggtt tgcccccgat tctttcggcc ctgactggaa
      961 aaaaacaacc caagggattg ttgtctttgc ccttcccaca cacaagaaaa tggtctagta
     1021 tcgaatttgt caatccgctg gctgaccatt cc
//
LOCUS       DAOQDO010002514         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_197258_length_1051_cov_3.280120, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002514 DAOQDO010000000
VERSION     DAOQDO010002514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197258_length_1051_cov_3.280120"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..>1051)
                     /gene="tssK"
                     /locus_tag="PKZ53_28380"
     CDS             complement(142..>1051)
                     /gene="tssK"
                     /locus_tag="PKZ53_28380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNJ44431.1"
                     /translation="GQALGNYQILVGPEIREGFSALKIGVITRSATGQFVLDEQFVPP
                     LLDSQGSAWLQTEFRKIIEILITKSSSLGDQRRQGANSMVDFTALEASAFWLLHTING
                     AIPKLKHLYATRAVHPERLYTELVGVIGQLMTFAPLRHPKDIVAYDHTDLRQTFGRLF
                     ADLRDLLEIVISTRCVAIPLTNVRESVYVGRVEDERLLQDASFFLAIRSQMPEREAIV
                     KAPRMIKVAAADHIDRVIGVAMPGVPLNYAAAPPGPIPTRPGFVYFGLETVGRFWDVI
                     RGSKSIGIYVPNEFPEVKLELYAVKP"
BASE COUNT          290 a          270 c          256 g          235 t
ORIGIN      
        1 cctgcccccc tgcccccgag cccctgatct caaaatggta taaaatacaa aaaacacaga
       61 gcaggctcgg aactcatcac gagtccgacg ctttctctgt gctgattgca atattaccgt
      121 tcagggatca gggcatcgag gtcaaggttt cacagcatat aactcaagtt tgacttcagg
      181 aaattcattg ggaacataaa tgccgatgga ttttgaccca cgaatgacat cccaaaatcg
      241 gccaacagtt tccaacccaa agtagacaaa acccggacgg gtggggattg gccctggagg
      301 tgccgccgca taattgagcg ggacaccagg catggccact ccgatgaccc gatcaatatg
      361 atcggcagcc gccactttga tcatgcgtgg ggctttgaca atcgcttcgc gctctggcat
      421 ctgggaacga attgccaaaa agaagcttgc gtcctgcagc aatcgctcat cttcaacccg
      481 accaacatag actgattccc gtacgttggt cagtggaatg gcaacgcatc gggttgaaat
      541 gacaatttcg agcaaatcgc gcaaatcagc aaacaatcgg ccaaaagttt gccgcaagtc
      601 ggtgtggtca taggccacaa tgtcttttgg atgacgcagt ggagcaaatg tcatcaactg
      661 tccaatcact ccgaccagtt cagtatacaa ccgttcagga tgaacagcgc gtgtggcata
      721 taaatgttta agtttgggaa tggcaccgtt gatggtgtgc aagagccaga aagcactggc
      781 ttccaaagcc gtaaaatcaa ccatggaatt ggcgccctgt cggcgttgat cgcccagact
      841 ggaactttta gtaatcagga tttcaatgat tttacgaaat tcggtttgca accaggcaga
      901 accctggcta tccagcaagg gtggaacaaa ctgttcgtcc aaaacaaact gcccggttgc
      961 gcttcgggta atcacgccga ttttgagagc tgaaaatcct tcgcggattt cagggccgac
     1021 caggatttga taattcccaa gcgcctgtcc a
//
LOCUS       DAOQDO010002515         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_197283_length_1051_cov_2.811245, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002515 DAOQDO010000000
VERSION     DAOQDO010002515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197283_length_1051_cov_2.811245"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..971
                     /locus_tag="PKZ53_28385"
     CDS             75..971
                     /locus_tag="PKZ53_28385"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ44432.1"
                     /translation="MSCPNVTQNLSAFVNDELSQAESRLVAEHLMQCLACRERYDEIK
                     LGSQLAEELPMMKAPDGMWAEIERALDGKKAKPANVSGWTLMRVLRFACVALSPVLVV
                     IAIVMIGVGHLKQKFEGPVVQVRVEQRALSDFERFGRELHQTLGSGTLKLDFLTSAPG
                     EIRRWVSDHTRLHAWVALDRPVEDGDFYKPVGVKKVLFKGAEAICVVYRVGTHPVTLL
                     AMNQQALPELPKERPHVVDVATQTDPESGLKVMSWAKDGQAYVVVSDLPESGQQGCFL
                     CHAQPERREQIRSGFQQGRNSR"
BASE COUNT          246 a          244 c          300 g          261 t
ORIGIN      
        1 acagggtgat ttttttcatc cctcatccct catccctcat cccttcagaa gccctgaacc
       61 ctatacccta aaatatgagc tgtccaaacg ttacccaaaa tttatcagct ttcgtcaatg
      121 acgaattgag tcaggctgag tctcgactgg tggctgagca tttaatgcaa tgcctggcct
      181 gtcgtgaacg atatgacgag atcaagttgg ggtcacaact ggccgaagaa cttcccatga
      241 tgaaagcgcc tgatggaatg tgggccgaaa tcgaacgggc acttgatggg aaaaaggcca
      301 aaccggcaaa cgtttcagga tggactttga tgcgagtgtt gcgctttgca tgtgttgcgc
      361 tttctcccgt gttggtggtc attgcaattg tgatgattgg tgttggacac ctcaaacaga
      421 aatttgaggg gccagtcgtg caggttcgtg ttgagcagcg agcgctctca gactttgaac
      481 ggtttggacg tgaattgcac caaactttgg gaagtggaac gctcaagctc gattttttga
      541 cttccgcacc gggtgaaatt cgccggtggg tcagtgacca tacccggctg catgcctggg
      601 tcgctcttga ccgacccgtt gaagatggcg acttttacaa accagtagga gtgaaaaaag
      661 ttctctttaa aggggctgaa gctatttgtg tggtgtaccg ggtgggaacg catccggtga
      721 cgctgctggc gatgaaccag caggcgctcc ctgagcttcc aaaggaacgc ccgcatgttg
      781 tggatgttgc cacccagact gatcctgaat cggggctcaa ggtgatgtca tgggccaagg
      841 atggtcaggc ctatgttgtg gtatcggatc ttccagaatc gggccagcag ggatgttttc
      901 tctgtcacgc ccaaccggaa cgccgggaac aaatccggtc gggctttcag caagggagga
      961 actcaagatg aaacgaaaca ttcaactttt ggcaattgtg gtgatggttg gaagtggcag
     1021 cctggcttta gactggcaca tcctggctga g
//
LOCUS       DAOQDO010002516         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_198411_length_1048_cov_3.654582, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002516 DAOQDO010000000
VERSION     DAOQDO010002516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198411_length_1048_cov_3.654582"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..414)
                     /locus_tag="PKZ53_28390"
     CDS             complement(274..414)
                     /locus_tag="PKZ53_28390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44433.1"
                     /translation="MLGTWGYTDIASHLSSTASQRLLIDSKPSQSINTSAARAILSGV
                     NE"
     gene            584..>1048
                     /locus_tag="PKZ53_28395"
     CDS             584..>1048
                     /locus_tag="PKZ53_28395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44434.1"
                     /translation="MNVSTSSKNVLVIVHDFPPLGGGWVIRALKFVKYLEFFGWRPVV
                     LTVDAAFYPPALLDQSLLDEISTSTPVYRTRTLIGPYQLQLPNQQAEAFSTRGTGPQF
                     RQILEFLVPESLRVIQDYGFFWLPPAWAQASKIINQEAINLIFTTSPPHNVHL"
BASE COUNT          258 a          259 c          250 g          281 t
ORIGIN      
        1 atatggcatt gattgccagt accacgacca caaaaatggc tccaatccct tgccaccagc
       61 ggatgctggg aaaagagcgg gctggtttcc actgttcaat ggccagcatg accagatagg
      121 taacgggaat cagtaatccc aacaaatctt caggtttcat aaacaaaacg ttcctccaaa
      181 atccagttaa atcagagtgg ttttcaaaaa tcaccttacg cggtaaggtc atggcgataa
      241 aatctcaaac tcccagaaag caagaacctc ggttcactca ttgacgccgc tcagaattgc
      301 ccgagcggca ctggtgttga ttgattgtga tggtttggaa tcaatgagta accgttggct
      361 ggcggtggaa ctcagatgtg aggcaatgtc ggtgtacccc cacgttccga gcaaaggtca
      421 tgttttggat ttagttgccg atttgttgat ggggaaattg agcggtcaga tgctggattt
      481 ttcagcactg gatagacgca aagttcttgc aactctgctc attcactggt aaagacgagg
      541 ttccttgccg ctctactctg cctcctggat tcactgtgta tccgtgaacg tatcaacctc
      601 atcaaaaaat gttctggtca ttgtccatga ttttcctccg ttgggtggag gctgggtcat
      661 cagagccctg aaattcgtca aatatctgga gttttttggc tggcggccag tggtgttgac
      721 tgttgacgcc gccttttatc caccagcgtt gctggatcaa tcattacttg atgaaatctc
      781 aacttcgacc ccggtctatc ggactcgaac cttaatcggt ccgtatcaac tccagctccc
      841 aaaccaacag gctgaggcgt tttcaaccag gggaactggc ccccagtttc gccagatatt
      901 ggaatttttg gtgcctgaaa gcctgcgtgt gatccaggat tacggttttt tctggctgcc
      961 acctgcctgg gcccaggcga gcaagatcat taatcaggaa gcaatcaacc tgatctttac
     1021 cacttcgccg ccacataatg ttcatctg
//
LOCUS       DAOQDO010002517         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_198852_length_1047_cov_2.727823, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002517 DAOQDO010000000
VERSION     DAOQDO010002517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198852_length_1047_cov_2.727823"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1047)
                     /locus_tag="PKZ53_28400"
     CDS             complement(54..>1047)
                     /locus_tag="PKZ53_28400"
                     /inference="COORDINATES: protein motif:HMM:NF023695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HNJ44435.1"
                     /translation="KRNRLDCFAPTGGVHFKAVKSFDLDHFLADSAATKSANGTAFDI
                     EIESAAFDGSSLWVVGSHSLSRILADPFKPDRKNILQLQSLVKKKHRFVLARIPVAIR
                     QEDETRLTVELLDDADPENVSRLAGTVDGNELTAAIAEDEHLQPFLAIPGDENGLAVG
                     GIAVYGNKVFLGLRGPVLHGWGIVLELEVEEDSDHILHLKPIGPDGQRYRKHFLQLDG
                     LGIRDLCLDGQDLLLLARPSQDFDWPVMIYRWYQGVTQVEERLVWSRQGSELTQVICG
                     PRTAVGTGYDRPHSLSLVSNDEFPYSVLVGYEAPSPYRLPRPAQLDLDLFRLTQ"
BASE COUNT          275 a          291 c          253 g          228 t
ORIGIN      
        1 cccggaaccc ggaacccgga acccggaacc cggaacccgg aacccggaac ccatcattgc
       61 gtcaagcgaa acagatccag atcaagctga gctggtcgag gcaggcgata gggagaaggc
      121 gcttcatatc cgaccagaac tgaataggga aactcatcat ttgagacgag cgagagacta
      181 tgtggccgat catatccggt gccaacggct gtccgtggac cacaaatgac ctgggtcagt
      241 tcgctgccct ggcggctcca gaccagtcgt tcttcaacct gggttacccc ttgataccac
      301 cgatagatca tcactggcca atcaaaatcc tggctgggtc gggccaggag caataaatcc
      361 tgcccatcca ggcacaaatc gcgaattccc aggccatcga gctggagaaa atgttttcgg
      421 tagcgttgcc catctggccc gattggcttg aggtggagta tatggtctga atcttcctca
      481 acttccagtt ccaaaacaat cccccagcca tgcagtacag ggccgcgcag tcccagaaac
      541 actttatttc catagacagc aatcccaccg accgccagcc cgttttcatc tccaggaatt
      601 gccagaaagg gttgaagatg ttcatcttcg gcaatggcag cagtgagttc attaccatct
      661 actgtacctg ccagccgtga aacgttttca ggatcggcat catccagtaa ctcgacggtt
      721 agccgggttt cgtcttcctg acgaatggcc actggaatcc gcgcaagcac aaaacgatgt
      781 ttctttttga ccagagactg gagttgaagg atatttttgc ggtcaggttt gaacggatca
      841 gccaggattc gactcaggct gtgtgaccca accacccaga gcgaactccc gtcaaaggca
      901 gcactttcga tttcaatatc aaaagcagtc ccgtttgcgg atttcgtcgc agcagaatca
      961 gcaaggaaat ggtctaaatc aaatgatttc acagctttaa aatgcacacc tccggttgga
     1021 gcaaaacaat ccagacgatt ccttttt
//
LOCUS       DAOQDO010002518         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_199203_length_1046_cov_3.553986, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002518 DAOQDO010000000
VERSION     DAOQDO010002518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199203_length_1046_cov_3.553986"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKZ53_28405"
     CDS             complement(<1..394)
                     /locus_tag="PKZ53_28405"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNJ44436.1"
                     /translation="MKTKKVGILIGGGPAPGTNGVIAAVVIGAINANCTPIGIHDGFE
                     WLAQRYTDEQHDMTIDEVSRIHLHGGVLLGTSRTDITRTDQTLANTISALKKLGIEML
                     VTVGGDDLVSSAVAVERESRGAIRVVQVP"
     gene            complement(452..>1046)
                     /locus_tag="PKZ53_28410"
     CDS             complement(452..>1046)
                     /locus_tag="PKZ53_28410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNJ44437.1"
                     /translation="AIIKRRATDHHYGVAVLAEGLIDKLDPQELAGLQDVERDDHGHI
                     RFAEVDLARKIKVEVQGRLSERGIRLTMTNKNLGYELRCADPIAFDAAYCRDLGHSAI
                     QFLVTGGNGAMVSVQDGKLVPLPFDTIRDPETGKTNIRLVDTQKESYRVARSYMIRLE
                     RDDFGHNAWVEQLAIAGNLTADEFRARFGYLAENTPW"
BASE COUNT          226 a          296 c          276 g          248 t
ORIGIN      
        1 ttggtacctg aacgacccga atcgcgccgc gtgattcacg ttcgaccgcc acggcgctgc
       61 tgaccagatc gtcaccgcca accgtgacca gcatttcaat acccagtttt ttcaaggcgg
      121 aaatggtgtt ggccagggtt tggtcggtac gggtgatatc ggttcgtgaa gttcccaaca
      181 ggacgccacc gtggaggtgg atgcgggaaa cttcatcaat tgtcatgtca tgttgttcgt
      241 ccgtatagcg ttgcgccagc cactcaaaac catcatgaat accaatcggc gtacagtttg
      301 cattgatggc accgataacg actgccgcaa ttacaccgtt tgtacctggg gccggaccac
      361 caccaattaa aataccaact ttctttgttt tcatagtttt gtgtccttgg agatgggagt
      421 gaagggttct gacctgctcc cagtctaaac ctcaccaggg ggtgttctca gccagatacc
      481 caaaccgggc gcgaaactca tctgccgtca gattgccggc aatggccagt tgctcgaccc
      541 aggcattgtg cccgaaatca tcgcgttcca ggcgaatcat atagcttcgg gcaacccggt
      601 aactttcctt ctgagtatca accagccgga tattggtctt gccggtctca gggtcgcgaa
      661 tggtgtcaaa cgggagcggc accagtttcc catcctgaac gctgaccatg gcgccgtttc
      721 cacccgtgac caggaactga attgcgctat gacccagatc acgacaatac gccgcatcaa
      781 acgcaatcgg gtccgcgcag cggagttcat acccaaggtt tttgtttgtc atggtcaggc
      841 gaatgccacg ctcgctgagt cggccttgga cttcaacttt gatcttgcgg gccaggtcaa
      901 cttcggcaaa ccggatatgc ccgtggtcgt cacgttcgac gtcctgcagg ccagccagtt
      961 cctgtggatc gagtttgtca atcaagcctt cggccaacac cgcgactcca taatgatggt
     1021 cagtggcgcg gcgtttgatg atggcg
//
LOCUS       DAOQDO010002519         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_199268_length_1046_cov_2.569122, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002519 DAOQDO010000000
VERSION     DAOQDO010002519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199268_length_1046_cov_2.569122"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..>1046
                     /locus_tag="PKZ53_28415"
     CDS             237..>1046
                     /locus_tag="PKZ53_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ44438.1"
                     /translation="MNTERIRNFSIIAHIDHGKSTLADRILEKTGALSSREMSEQVLD
                     AMDLERERGITIKAHAVRLSYRAQDGEDYILNLIDTPGHVDFSYEVSRSLAACEGALV
                     IVDASQGVEAQTLANAYLALENNLEMIPVINKIDLPSADPDRVLEQIEQVIGLDSSSA
                     VLTSAKTGQGVEDVLEAIIQHIPPPTGSREEPLKALIFDSWFDPYRGVIVMIRLIDGI
                     IRPGMKFRFMATGREYQVEGLGVLTPKAQSINELGAGEVGFIVSNIKTISDV"
BASE COUNT          254 a          269 c          261 g          262 t
ORIGIN      
        1 gcacaaaaag gaaacggcct gggctgtttc cttttttctt ttgcttttta ttgaataact
       61 taactgcctt taggaatgaa aggttgggct ttgccggtaa ataagatata ttgcgtggtt
      121 ttcatcacta cagtttgaca gcctggatgt gctttgtgga aagcacgttt ccaggatcgg
      181 gctgccagag ggcaggtaca tttctacttc caaggacttt ttctccaaac ggttttatga
      241 acacagaacg aattcggaat ttttcaatca tcgcccatat tgaccacgga aaatcaacgc
      301 tggccgaccg catcctggaa aaaacggggg cgctttcctc acgtgaaatg tccgaacagg
      361 tcctcgacgc catggatctt gaacgagaac gcggcattac catcaaagcc cacgccgtgc
      421 gactcagcta tcgcgctcaa gatggcgaag actatatcct gaacctgatt gacacgcctg
      481 gtcacgttga tttctcgtat gaagtttccc gctcactggc ggcgtgtgaa ggggcactgg
      541 tgattgtgga cgcgtctcag ggagtggaag cccaaaccct ggccaatgcc tatctggccc
      601 tcgaaaataa ccttgagatg atcccggtga tcaacaaaat tgatttgcct tcagccgacc
      661 ccgaccgggt gctcgagcaa atcgaacagg tcattgggct tgactcttcg agtgccgttc
      721 tgaccagtgc caaaaccggc cagggcgtgg aagatgtcct ggaagccatt attcaacaca
      781 ttccgccccc aaccggcagc cgcgaggaac cgctcaaagc actgattttt gattcctggt
      841 ttgatccata tcgcggcgtc attgtcatga tccgactcat tgacggcatt attcgacccg
      901 gaatgaagtt ccggtttatg gcaacgggcc gggaatatca ggttgaaggt ctcggtgtcc
      961 tcacccccaa agcccagtcc atcaacgaac tgggtgctgg tgaagtcgga tttattgtct
     1021 cgaatatcaa aaccatctct gacgtg
//
LOCUS       DAOQDO010002520         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_199369_length_1046_cov_1.949546, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002520 DAOQDO010000000
VERSION     DAOQDO010002520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199369_length_1046_cov_1.949546"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..502
                     /locus_tag="PKZ53_28420"
     CDS             149..502
                     /locus_tag="PKZ53_28420"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNJ44439.1"
                     /translation="MPVKEISTKSFESEVFESGQPVLVDFYAPCCSPCRALIPVLEQL
                     DAEFAGRVKIVKINVDKNRDCVGRFELAKIPTLMMFSDNESKDTIWGMKTFPVLRQKV
                     DAWLAATSLVPEPAR"
     gene            547..819
                     /locus_tag="PKZ53_28425"
     CDS             547..819
                     /locus_tag="PKZ53_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HNJ44440.1"
                     /translation="MLYMVIERFKNQDALPVYRRFREQGRLAPAGLHYVSSWVDEQFQ
                     CCYQVMETDDRALLDEWTANWSDLVDFEVIPVMTSKEAVERISPRL"
     gene            complement(860..>1046)
                     /locus_tag="PKZ53_28430"
     CDS             complement(860..>1046)
                     /locus_tag="PKZ53_28430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module antidote protein, HigA family"
                     /protein_id="HNJ44441.1"
                     /translation="SEILHERRPVTIDLAHRLARVFCTTPELWVNLQQAVDLWDELQA
                     HKDEYEKIKPIETGEAA"
BASE COUNT          267 a          253 c          255 g          271 t
ORIGIN      
        1 tcgggaggga acacatctca aggggttggc atccaatttg cgtagaaaat agtcattcag
       61 gcagatttaa attccgaaga caataccttt cggatcgcct gaccatccag gaagccattg
      121 aacttcaaag ttgcaggaga tgtcagatat gccagtcaaa gagatctcaa ccaaaagttt
      181 tgaatctgaa gtttttgaat caggtcaacc agtactcgta gatttttatg ctccgtgttg
      241 cagcccctgc cgggcgctca ttccggttct cgaacagtta gacgccgaat tcgccgggcg
      301 ggtcaaaatc gtcaagatca atgttgataa aaatcgtgac tgtgttggtc ggttcgaact
      361 tgcaaaaatc ccaactctga tgatgttttc cgacaatgaa tccaaagaca cgatttgggg
      421 catgaaaacc ttcccggtgt tgcgccagaa agtagacgcc tggctggccg caacttcgtt
      481 ggttccagaa ccagcccggt aaattcaggc acatctcgta ttctattcgg agaacctttc
      541 ttttctatgc tttacatggt catcgaacgc tttaaaaacc aggatgccct gccggtctat
      601 cgtcgcttca gagaacaagg acgcttagca cccgcagggc ttcactatgt ctcaagctgg
      661 gtggatgagc agtttcagtg ctgctatcag gtgatggaaa ccgatgaccg agccttgctt
      721 gacgaatgga ccgcgaactg gagcgatctg gttgattttg aagtgattcc ggtcatgaca
      781 tcaaaagagg ccgtggagcg catttcacca cggctttgaa ctggtaacca atcaccagaa
      841 cccttttgtt tccaactgac taggcagcct ctcctgtctc aattggtttg attttctcat
      901 attcatcttt atgagcctga agttcgtccc aaagatcaac cgcctgctgc aaattcaccc
      961 atagttcggg agtggtgcaa aagacacgtg ccagccgatg ggcaaggtca atcgtgactg
     1021 gtcggcgttc gtggagaatc tcgctg
//
LOCUS       DAOQDO010002521         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_199424_length_1046_cov_1.746720, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002521 DAOQDO010000000
VERSION     DAOQDO010002521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199424_length_1046_cov_1.746720"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1046)
                     /locus_tag="PKZ53_28435"
     CDS             complement(<1..>1046)
                     /locus_tag="PKZ53_28435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44442.1"
                     /translation="EREWVCDEAVLCGSVTAQTYAASLWKVAQFSLGWPQTKVAGVAR
                     VSGSNLKRRIEAMLQNEFPKRLAWPQRFWLGLTVAALVMVAGVLALPPQTPAFAQIAL
                     LDADKVNLAEAAQPGIPNQPESKRKLGMKREMTEEKTGVAVSPAASLEPQAAANASQT
                     KLPDNLDQWPEYSVLPVNPAGAPLLVTRASIHALPKPPGLPSLGARLGQAEHDEWWSY
                     VNFTLNNTTAKGIARMVFTLENPAFEETRVRHVLTFGDRNSVLIEPLDNYATRKGIDN
                     RPVTQFQIKKTSPFNPAAFSLRLTLVEFTDGSSWQLAWDANQTALQTSLQQLKSSSQE
                     LSVSQSTSQEDEPL"
BASE COUNT          215 a          285 c          297 g          249 t
ORIGIN      
        1 aagcggctcg tcttcctgtg acgtgctttg actgaccgac agttcctgac tggatgattt
       61 caactgttgc agtgaggttt gcagcgccgt ttgattggca tcccaggcaa gttgccagga
      121 agacccatct gtaaattcca ccagcgtcag gcgaagcgag aaggcggcgg ggttgaaagg
      181 actggttttc ttgatctgaa actgtgtgac cggacgatta tcaatacctt tgcgcgtggc
      241 atagttatcc aaaggctcaa tcagcactga atttctgtcg ccaaacgtca gcacatgacg
      301 cacccgggtt tcctcaaaag ccggattttc cagcgtgaag accatccgcg cgatcccttt
      361 ggcggttgtg ttgttgaggg tgaaattgac gtacgaccac cattcatcgt gttctgcttg
      421 tcccagcctg gcaccgagcg atggcagtcc aggcggcttg ggcagggcgt gaatgctggc
      481 cctggttacc agcaagggag cgcctgcggg attgacgggc aacacggaat actcaggcca
      541 ttgatcgagg ttgtccggga gtttcgtttg agacgcgttg gccgcagctt gaggttcgag
      601 gctggctgcc ggggataccg caacgccggt tttctcttcg gtcatttcac gcttcatccc
      661 caatttcctt ttgctctcag gttggttggg aattcctggt tgggcagctt cagcaagatt
      721 gactttgtct gcatccagca aggcaatttg tgcgaaggcc ggagtttgcg gcggcaaggc
      781 aagcacgcca gcaaccatga ccaaagctgc gactgtcaac cccagccaaa accgctgcgg
      841 ccaagccaac cgtttgggaa attcattttg taacatcgct tctatcctcc ttttgagatt
      901 ggaaccgctg acgcgcgcga cgccagcgac tttagtttgc ggccaaccca ggctgaactg
      961 ggcgactttc caaaggctgg cggcgtaggt ttgcgcagtg accgacccgc acaacaccgc
     1021 ttcatcgcaa acccattcgc gttccg
//
LOCUS       DAOQDO010002522         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_200267_length_1044_cov_1.364004, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002522 DAOQDO010000000
VERSION     DAOQDO010002522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200267_length_1044_cov_1.364004"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..>1044)
                     /locus_tag="PKZ53_28440"
     CDS             complement(283..>1044)
                     /locus_tag="PKZ53_28440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44443.1"
                     /translation="YERGLYFSTDTGKTWQRLAEMDALAVYFISPQIGYAAGNYGALL
                     KTENGGKTWARCGSLEATLNKSVKLQDAIFSFTRVKFLDSQTGWAIGNYYGTGTVEAG
                     GLFTTQDGGQTWEKAGVPLTASRAELMNFHFFDKLHGYLTAEAYQGDSRYLTQFQTAD
                     GGKTWIERTTTFPGYHVTTFTNPSTGWTIGNRPLRLNGPTFETGFWKTQDGGQTWKEE
                     TALPGMRLYDVFFLESGVGWAVGERGLVLKYVPRS"
BASE COUNT          253 a          279 c          244 g          268 t
ORIGIN      
        1 aaaaggttaa actaactaaa ttattcattt gtaacaagat aactcgtcct ggtggaaata
       61 agctttaacc aaatggtgaa aacctgcggt gtggtttcac aggcttactc agactcaaaa
      121 aagaaaccac gaagcaagcg gaatccagtt ccaattgctt cgtggtttct tctgatcacg
      181 cttgtgcctg acagtcatcc catatttaaa aacaggattt cgctatcttg ctcttccagc
      241 ttgactactt tgctacttca ctactttgct acttcactgt ccctagcttc gcgggacgta
      301 cttgagcacc agtccacgct cgccgacggc ccagcccaca cctgattcca aaaagaacac
      361 atcatagagt cgcattcccg gcagggcggt ttcttctttc caggtctggc cgccatcctg
      421 cgttttccag aacccggttt caaatgttgg gccattgagt cgcaacggac gatttccaat
      481 cgtccagccc gtggatgggt tggtaaaagt ggttacatga tagccaggga aggtcgtggt
      541 ccgctcaatc caggttttgc cgccgtctgc cgtctgaaat tgtgtcaaat agcgactatc
      601 gccttgatag gcttcggcgg tcagatagcc gtgcagcttg tcaaagaagt gaaaattcat
      661 cagttcagct cgcgaggccg tcagcggtac accagctttt tcccaggttt gaccgccatc
      721 ttgagtggta aaaaggccgc cagcctcgac cgtgcctgtg ccgtaataat tcccaattgc
      781 ccagccggtt tgggaatcaa ggaatttgac cctggtaaaa gagaaaatgg catcctggag
      841 cttgaccgac ttattgaggg ttgcttcgag tgatccacag cgagcccagg tcttgccacc
      901 attctcagtt ttgagcaacg cgccataatt accggcggca tacccgattt gagggctgat
      961 aaaatacacc gccagcgcat ccatttccgc cagtcgctgc caggttttgc ctgtatcggt
     1021 gctgaaataa agcccccgtt cata
//
LOCUS       DAOQDO010002523         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_201496_length_1040_cov_3.718782, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002523 DAOQDO010000000
VERSION     DAOQDO010002523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201496_length_1040_cov_3.718782"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="PKZ53_28445"
     CDS             complement(<1..>1040)
                     /locus_tag="PKZ53_28445"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ44444.1"
                     /translation="LPQLWGGIFDLDPHFTPGEAVEAACTAMFNSTPEDQLVFRKDGL
                     YGLRLVTVPPDSKPQPSLVWRTNASYLITGGLGGLGLLVAEWMAQQGARRLILTSRTP
                     LPARSEWKTYPKDSPQGQQIAAVRTLEAAGVHVQTVAIDVSDAEQLGQFLRTYADEGW
                     PPIRGVIHAAGVLEDQTLLNLNREALLAVTRVKILGTWHLANAFSPQTLDFLVNFSSL
                     ASVLGSPGQANYAAGNAFQDAMTHWLGRNGYQATTINWGPWAEAGMAARNVSEGKSPT
                     PGIDLLSPAMGLQILHHALNAQVPTLIAARCDWERLNHLFPTLTTSPLLSQITEAPGP
                     VASPPVQTAPLP"
BASE COUNT          216 a          283 c          313 g          228 t
ORIGIN      
        1 caggaagggg ggctgtctga actgggggtg acgccaccgg gcctggtgct tcggttattt
       61 gactgagcaa tggagaggtg gtcagggtcg ggaaaaggtg gttgagtcgt tcccagtcac
      121 accgggctgc aatcaaagtc ggcacctgag cattcaaggc gtggtgcaga atctgcaatc
      181 ccatagctgg tgacaggaga tcaatccctg gagtcgggct ttttccctcc gagacattgc
      241 gcgcggccat gccagcttca gcccacggcc cccagttgat ggtcgtggcc tggtagccat
      301 ttcgacccaa ccagtgggtc atggcatcct gaaatgcatt gccagccgca taattggcct
      361 gaccgggcga tcccagtacg gaggccagtg aactgaaatt gaccaggaaa tccagtgttt
      421 gcggcgaaaa tgcgttggct agatgccagg ttcccaggat tttgactctg gtgaccgcca
      481 gtaacgcttc tcgattgaga ttaaggagcg tctggtcttc gagcacgccc gcggcgtgaa
      541 tcactccccg aattggcggc cagccttcat cggcataggt tcgcagaaat tgcccgagtt
      601 gttcagcgtc tgaaacatca atcgccactg tctgcacatg aacgcctgcc gcttcaaggg
      661 tgcggacggc tgcaatctgt tgtccctgtg gcgaatcttt cgggtatgtc ttccattcac
      721 ttcgggctgg aagcggtgtc cgactggtca aaatcaggcg ccgggcaccc tgttgggcca
      781 tccactcagc cacgagcaac ccgagtcctc caagtcctcc ggtgatgaga tagctggcat
      841 tggttcgcca gaccagcgac ggctggggtt tggaatctgg tggcacggta accagtcgga
      901 ggccatacag gccatctttt cgaaacacca gttgatcttc aggtgtcgaa ttgaacatgg
      961 ccgtgcaggc tgcctcgacg gcttcaccag gggtaaagtg tgggtcgaga tcaaatatcc
     1021 ctccccacaa ctggggaagt
//
LOCUS       DAOQDO010002524         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_201505_length_1040_cov_3.302538, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002524 DAOQDO010000000
VERSION     DAOQDO010002524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201505_length_1040_cov_3.302538"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKZ53_28450"
     CDS             <1..464
                     /locus_tag="PKZ53_28450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNJ44445.1"
                     /translation="DQSSLSRSGLFFDLHTLIGGQFEKDETHKLYLVYPQGNWVEILT
                     DTPFCIIGRSAYGKPLLDRMLTYPTSLEHALNIGFLAFDSTCTSVTEVDFPIDIALYR
                     SGSFAISEHRFAQNELQEASEWWHTRLSELVKDVPSNWAKKFFSKSEAAHS"
     gene            complement(652..>1040)
                     /locus_tag="PKZ53_28455"
     CDS             complement(652..>1040)
                     /locus_tag="PKZ53_28455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44446.1"
                     /translation="MTISDEGPHLDLLNWKHGVSPWVELTSLQPDVFRGNPLTEKDMN
                     RFPAYTREELYQAIIAQEKKNKMTYGDLYSQNEERWLRIVKQKPQGKYLPYSPEVSTI
                     RLRIQAKEGDTWQVIHTVEIAVALGC"
BASE COUNT          255 a          257 c          241 g          287 t
ORIGIN      
        1 aagaccagtc atcgctttca cggagcggcc tgtttttcga cctgcacacg ttaattggcg
       61 gccagtttga aaaagacgag acccacaaat tgtatctggt gtatccgcaa ggcaactggg
      121 ttgaaatcct gaccgacacg ccgttttgca tcattggccg ctcagcgtat ggaaaaccgc
      181 tgctggatcg gatgctgacc tatccgacca gcctggaaca tgcgctcaac attgggtttc
      241 tggcgtttga ttcaacctgc accagcgtga ccgaagttga ttttccgatt gatattgccc
      301 tctatcgcag cggctcgttt gcgatttctg aacatcggtt cgcccaaaat gaactgcagg
      361 aagcttctga atggtggcac acccggctca gtgaactggt taaagacgtg ccatcaaact
      421 gggcgaaaaa gttcttttca aagagcgaag cggcgcattc ttgaaaaaca ggaaggacaa
      481 aaaagacgaa ccaaatcgaa ccagaagaga ttggaacaca tcgggttcag ggttcagggt
      541 ttgatccgaa gcagtcgaac tggccagcga cttaacaagg attggttttc ctttttgtct
      601 tttttgtcct tctgatcctg tttgtccttt cgacccctga accctcaatt gctaacaccc
      661 cagtgccacc gcaatttcca cggtatgaat gacctgccag gtatcgcctt ctttcgcctg
      721 gatccggagt cgaatggtac ttacttcagg gctataaggc agatatttcc cctgtggttt
      781 ttgctttaca atgcgtaacc acctttcctc attttgagaa tagagatctc catacgtcat
      841 tttgtttttt ttctcctgag cgatgatcgc ctgatataac tcctccctgg tataagcagg
      901 aaatcggttc atatcttttt ctgtaagggg atttcctcgg aaaacatctg gttggagtga
      961 ggtgagttca acccagggag acaccccatg tttccaattt aaaagatcaa gatgtggccc
     1021 ctcgtcactg attgtcatac
//
LOCUS       DAOQDO010002525         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_201651_length_1040_cov_2.118782, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002525 DAOQDO010000000
VERSION     DAOQDO010002525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201651_length_1040_cov_2.118782"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="pdxT"
                     /locus_tag="PKZ53_28460"
     CDS             complement(<1..287)
                     /gene="pdxT"
                     /locus_tag="PKZ53_28460"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNJ44447.1"
                     /translation="MKIGVLALQGDFAAHGRMLTKLGYDWCEVRRPVQLDETDGLILP
                     GGESSTMIKFIREEGFLEPLREYARSGKVMFGTCAGAILLANQVTNPEQESL"
     gene            complement(410..>1040)
                     /locus_tag="PKZ53_28465"
     CDS             complement(410..>1040)
                     /locus_tag="PKZ53_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNJ44448.1"
                     /translation="HFVEAQILEELGVDFIDESEVLTPADHANHINKFAFKVPFVCGA
                     TNLGEALRRIGEGAAMIRTKGEAGTGNVVEAVKHLRQIHRDMRLLTVLSEDELMAEAK
                     RHQAPFELVQLVAKLGKLPVPNFAAGGIATPADAAMCMQLGAETVFVGSGIFKSSDPA
                     RRARAIVKAATHFNDPKVLLEVSEDLGEPMRGIDVTTLPEGELLATRGW"
BASE COUNT          237 a          290 c          249 g          264 t
ORIGIN      
        1 agtgattcct gctctggatt agtcacctga ttggccaata aaatcgcacc agcacaggtg
       61 ccaaacatta cttttccaga tcgggcatat tcgcgaagcg gctctaaaaa accttcttcg
      121 cgaatgaact tgatcatagt tgagctttca ccgccgggca aaatcagacc atcagtttca
      181 tccagttgga ctggacgtcg aacttcgcac cagtcgtacc ccagtttagt gagcattcga
      241 ccgtgtgccg caaaatcacc ttgaagcgca agtacaccta ttttcataac tttaaaattc
      301 cgggttccgg gttttcggaa gggctgaggg ctgagggttg aagatttggt tttctttcat
      361 ccctcatcct tcatccctca tccctttaaa gaacccggaa ccctaaaaat taccatcctc
      421 gcgttgccag gagttcacct tccggcagtg ttgtcacgtc aattccgcgc attggttcgc
      481 cgaggtcttc gctgacttca agcagaactt tcgggtcatt aaagtgggtt gccgctttga
      541 caattgcccg tgcccgtcgc gccggatcag atgacttaaa gattccagag ccgacaaaca
      601 ctgtttccgc accaagttgc atacacatcg ccgcatcggc tggtgtagcg attccgcccg
      661 ccgcaaaatt tggaactggc aatttaccca gtttggcaac cagttgcacc agttcaaacg
      721 gggcctggtg ccgcttggct tcggccatca attcgtcttc actgagcacg gtcagcaacc
      781 gcatatcacg atggatctgg cgcaggtgct tgacggcttc gacgacgttt ccggtgccag
      841 cttcgccctt ggtgcgaatc atggcagcgc cttcgccaat ccggcgtaaa gcttcgccca
      901 aattggtggc gccgcagaca aacggcactt taaacgcgaa tttattgatg tggttggcat
      961 ggtctgccgg cgtcagcact tcggattcgt caatgaaatc aacacccagt tcttcgagga
     1021 tctgtgcttc gacaaagtga
//
LOCUS       DAOQDO010002526         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_202201_length_1039_cov_1.493902, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002526 DAOQDO010000000
VERSION     DAOQDO010002526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202201_length_1039_cov_1.493902"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKZ53_28470"
     CDS             complement(<1..773)
                     /locus_tag="PKZ53_28470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44449.1"
                     /translation="MPLTPTRAYFRPRNLKVLGMAALSSLLVWPAALPVSTTSAHSSA
                     PSAPLASALPPASAVSSDKLNELREDYQKLPMSFEVNQGQAHSKVKYLSHSSGYNVLF
                     TNNEVLLTMDATPPVKKTGKGKSAAKSTAPVADTVSLQFKGGNPSARIYGDRMMGKAH
                     YFVGNDPAKWLRNITTYHNVRYEKVYPGVDVVYYGHDKKLEYDVIVAAGADPKTVRLN
                     FGGVKNMRLNQNGELELTTAHGVVCQQKPVAYQLVNKLR"
BASE COUNT          244 a          247 c          281 g          267 t
ORIGIN      
        1 tgccgcagtt tgttcaccag ttgataggca accggttttt gttgacagac cacgccatgc
       61 gcggtggtca gttccagttc cccgttttgg ttgaggcgca tgtttttgac accgccgaaa
      121 ttcagccgca cggttttcgg atcggctccg gcagccacaa tcacgtcata ttccagtttt
      181 ttgtcgtggc cgtaatacac cacatccacc ccgggataga ctttttcata gcggacgttg
      241 tggtaggtgg tgatattccg caaccatttc gccggatcgt ttccaacaaa gtaatgggct
      301 ttgcccatca tccggtcacc gtaaatccgg gcggaagggt ttcctccctt aaattgcagg
      361 ctgacggtat cggccaccgg ggcggttgat ttggctgccg attttccctt cccggtcttt
      421 ttcaccggag gggtggcgtc cattgtcaaa agaacttcat tgtttgtgaa taaaacattg
      481 taaccactgc tgtgtgataa atacttaacc ttggaatgag cctgtccttg attgacttca
      541 aaactcattg gaagtttttg atagtcttcc cgcaactcgt tcaatttatc ggagctgacg
      601 gctgaagcag gaggcagggc tgaggcaaga ggggcagatg gggcacttga atgggcactg
      661 gttgtggaaa ccggcagggc agccggccaa acaagcaggc tgctcagggc ggccatcccc
      721 agcaccttca gattgcgcgg tctgaaatac gctcgcgttg gtgtaagtgg catgggtgta
      781 ctcctttgag actgtagggt cgaaagcaat tccaggttag aaatctggat tgcggaacct
      841 ctgtttgaga gctccgcttt cgaacaaaaa ctgtttccgg ttgaagaaag caggtcaaag
      901 agcagtgtgt tcggtttatc ccagccgaaa caatagcctt gaaaaaccaa accgaggcaa
      961 gaaaatcaaa gaaaaacctg ggatgagtgc gtgaaaggta cttccctttc gggattgaag
     1021 ttgatacccg gtatatccg
//
LOCUS       DAOQDO010002527         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_202285_length_1038_cov_3.479145, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002527 DAOQDO010000000
VERSION     DAOQDO010002527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202285_length_1038_cov_3.479145"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1038
                     /locus_tag="PKZ53_28475"
     CDS             16..>1038
                     /locus_tag="PKZ53_28475"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ44450.1"
                     /translation="MVKRLSAVETLGATTVILTDKTGTLTENEMTIREVWASGTHFTI
                     SGVGYGVEGVVEPSSHEATVVELLRTAALCCDARLVPPNAERTHWGIIGDPMEAAILV
                     AATKVGLTEEKLTAWPRLVELPFDSTRKRMTIIHQIDGQPVACVKGVPSQLLARSSRI
                     RWNGTTRPFDDAHQQTIQTIHDTIARRGLRLLGVAIRELNTSDQPVEGWQVEEVESDL
                     TFLGLLAMEDPPRPEVPAALAACREAQIQVVMVTGDNGLTATAIAQEIGMYQGEVQVI
                     TGDQLDHTSRPELLHLLDNPNVLIARATPEHKLRLVEAFQQRGEVVAVTGDGVNDAPA
                     LKRADIG"
BASE COUNT          246 a          293 c          289 g          210 t
ORIGIN      
        1 gctcaacgaa atgctttggt caaacgtctc tcggcggttg aaacactcgg tgccaccacg
       61 gttattctga cggacaaaac cggcacactg accgaaaatg aaatgacaat ccgtgaagtg
      121 tgggccagcg ggacgcactt taccatcagc ggcgtgggct atggagtcga aggcgtggtt
      181 gaaccttcca gtcacgaagc aaccgtggtt gaactcctca gaacagcggc actctgttgt
      241 gatgcccgac tggttcctcc caatgcagag cgaacccact ggggcattat cggcgaccca
      301 atggaagcgg cgatcctggt ggcagccacc aaagtcggtt tgacagaaga aaagctcacg
      361 gcctggcccc ggctggttga acttccattt gattcgaccc gcaaacgcat gacaatcatc
      421 caccagattg atggccagcc agttgcctgc gtcaaaggag tccccagcca gttactggct
      481 cgcagttcaa gaattcgctg gaatggcaca acacgtccat ttgatgatgc gcaccaacaa
      541 accattcaga ccattcacga taccatcgcc cgtcgtggcc tgcgcctgct cggagttgcc
      601 attcgtgaac tcaacacctc tgatcaacca gttgaagggt ggcaggttga agaagtcgaa
      661 tcagacctga catttttagg gctgctggcg atggaagatc ctccacgtcc cgaagttccg
      721 gcggcgctgg ctgcctgtcg ggaggcacag attcaggtgg tgatggtcac cggggacaat
      781 ggtctgaccg ccaccgcgat tgctcaggag attggaatgt accagggtga agtccaggtc
      841 atcaccggcg accagcttga tcacacctca agaccagaac tgcttcacct gcttgataat
      901 cccaatgtgt taattgcccg tgcgacgccg gagcataaac tccgactggt cgaagccttt
      961 cagcagcgcg gagaagtcgt ggccgttacc ggtgacggcg tcaatgatgc cccggctctc
     1021 aagcgagcgg atattggg
//
LOCUS       DAOQDO010002528         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_202352_length_1038_cov_2.530010, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002528 DAOQDO010000000
VERSION     DAOQDO010002528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202352_length_1038_cov_2.530010"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1038
                     /locus_tag="PKZ53_28480"
     CDS             <1..>1038
                     /locus_tag="PKZ53_28480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44451.1"
                     /translation="QARTDKLFQAFAADLRFQRVVATGDVTRMSRDEGWRYDVQLLAR
                     EVKRKGYAPFRHISREAFELNVQKIHESIPRLTDMQIVIELMKLMAQVGDGHTMIYGF
                     WERPEFLQTIPVEFSFFKEGLFVIAADSRFKELLGAKVLCFGHQTVDQIVAALDPLTN
                     RDNAMAPKVMAPLRMRNLPLLHGLGLIPDPTEVSLTVLDQKGAVHVFRLPADSGISSR
                     KLWDGLPKNWISFLQTIPGPVPLYLKNQGADYWFEYVPEPKTIYFQFNRVRNSEQESF
                     AAFCDRLFTFIQQQDVEKLVIDMRWNNGGDTTLVTPLIHCLIRAEKISRYGQLFVIVG
                     RRTFSAAQNAAT"
BASE COUNT          235 a          257 c          278 g          268 t
ORIGIN      
        1 caggcacgaa ccgacaagct gttccaggct tttgccgctg acttgcgatt tcagcgggtg
       61 gtcgccactg gtgacgttac ccggatgtct cgtgacgaag ggtggcgcta tgacgtgcag
      121 ctcctggcac gagaagtcaa acgcaagggg tatgctccgt ttcgccatat ctcccgcgaa
      181 gcgtttgaac tcaacgttca gaaaattcat gaatcaattc cacggctgac agacatgcaa
      241 atcgtgatcg aactgatgaa attgatggct caggttggtg atgggcatac catgatctat
      301 ggcttttggg agcgaccgga atttcttcag accattcccg tggaattctc ttttttcaaa
      361 gaaggtctgt ttgtgattgc cgctgactcg cggtttaaag agttattggg agctaaggtg
      421 ctttgcttcg gccatcaaac cgtagaccag atcgtggcgg cgctcgaccc actgacaaat
      481 cgtgataatg cgatggctcc aaaagtcatg gcgccgctcc gtatgcgaaa tctgccgctc
      541 ctgcacggcc ttggtctcat tcctgatcca acggaagttt cgttgaccgt tctggatcaa
      601 aagggagctg ttcacgtctt caggctgcct gctgattcgg gcatttcaag tcggaaactc
      661 tgggatgggc tcccgaaaaa ctggatcagt tttctgcaga caattccagg tccagtgccc
      721 ctgtatttga aaaatcaggg ggccgattac tggtttgaat atgtgccaga gccaaaaacg
      781 atctactttc aattcaaccg tgtgcgcaac agcgaacagg aatcatttgc cgcattttgc
      841 gaccggttat ttacctttat ccagcagcaa gatgttgaga agctggtcat tgatatgcgc
      901 tggaacaatg gtggcgatac cacgctggtg actccgctca tccattgcct gattcgggct
      961 gagaagatca gccggtacgg gcagcttttt gttattgtcg ggcgacggac cttctctgcg
     1021 gctcagaatg ctgcgacc
//
LOCUS       DAOQDO010002529         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_203081_length_1036_cov_3.786952, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002529 DAOQDO010000000
VERSION     DAOQDO010002529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203081_length_1036_cov_3.786952"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1036
                     /locus_tag="PKZ53_28485"
     CDS             <1..>1036
                     /locus_tag="PKZ53_28485"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ44452.1"
                     /translation="TVRRVVERAGMLLPISPKAFDVLLVLVEKAGEVISKDELLTLVW
                     PDTVVEEANLTQKIFHLRRVLGEKRTDHRYLVTVPGRGYRFVASVKPGAESATSTTTE
                     PASVTGVAEMSAPTLPALAVLPFTPLTQNGPEDLLAVGIADALIGKLLTTQKLIVRPT
                     SAVLTYRNSLKLPGVIGHELQVTQLLTGTIQLLENQIRLMVQLIHVSTGATVWAHPVN
                     AQIGHLFAVQDSLAAHLAEVLSLKLTQDEAQQMAKSYTLNLAAYEQYLRGKFFWNKRT
                     HVGLVNGAECFQRALEIDPTYALALIGLANSYLIMGEYLFLPPQVAFPKARQYALTAL
                     ELDPHLSTAHG"
BASE COUNT          238 a          273 c          275 g          250 t
ORIGIN      
        1 ataccgtgcg gcgcgttgtg gaacgtgcgg gcatgctctt gccaatttcc ccaaaggcat
       61 ttgatgtgtt gctggtcctg gttgaaaaag ctggtgaagt gatttccaaa gatgagttgt
      121 tgacgctggt ttggcccgat acggtggttg aagaagccaa cctcacccaa aagatctttc
      181 atttgcgccg ggtgctgggg gaaaaacgaa ccgaccaccg gtatctggtg acggtgcctg
      241 ggcgcggcta tcgctttgtg gcgtcagtaa aacccggtgc tgaatcagcg acttcaacca
      301 cgacagaacc ggcttcagtc accggtgtgg ccgagatgag tgcccccacc ttacccgcgc
      361 tggcggtcct ccccttcacc ccactgacac aaaatggtcc agaagacctg ctcgcggtgg
      421 ggattgccga tgccttgatt ggaaaacttc tgacgaccca gaagttaatt gtgcgaccaa
      481 catcggcggt gctgacctac cgaaacagcc tgaagttgcc aggtgtcatc gggcacgaat
      541 tacaggtgac tcaactgttg actggtacca ttcaattact tgaaaaccag atccgattga
      601 tggtgcagct catccatgtc tcaaccggcg caaccgtgtg ggcccatccc gtcaatgccc
      661 aaatcggtca tttgtttgcc gttcaggatt cattggcggc acatctggct gaggtgttgt
      721 cgttgaagct cacccaggat gaagctcagc aaatggccaa aagttatact ttgaatcttg
      781 ccgcctatga gcagtatctg agaggaaaat ttttttggaa taaacgaacc catgtcgggc
      841 tggtcaatgg ggcagaatgt tttcaacggg cgctggaaat tgatccgacc tatgccctgg
      901 ccctgattgg attggccaac agctatttga ttatgggaga gtatctcttt ctcccacctc
      961 aggttgcatt tcccaaagcc cgtcaatatg ccctcacggc acttgaactt gacccgcatt
     1021 taagcacggc ccacgg
//
LOCUS       DAOQDO010002530         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_204735_length_1032_cov_3.144319, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002530 DAOQDO010000000
VERSION     DAOQDO010002530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204735_length_1032_cov_3.144319"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..>1032
                     /locus_tag="PKZ53_28490"
     CDS             488..>1032
                     /locus_tag="PKZ53_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44453.1"
                     /translation="MMKTGELRPIFFDPDHRRWNWFRWTIKLVGYTLGGLLAVLGISF
                     LIDPVLPQLTLAQFKLANSGHHPVKPEPIQPEIGSQKHKARVGESRTASPHPEPSPSQ
                     PVSLSQASQSNEVIGFLVNWDDTSFTSLKQNSGHLTKLIPEWLHLADADGNITQDEPQ
                     KQQQVLAFLRSHQPQIKVVPL"
BASE COUNT          279 a          263 c          235 g          255 t
ORIGIN      
        1 taccgaaaat ttccagacat tttcaccacg gaaaacacgg aaaacacgga aaaaatcaac
       61 cacttaccaa acccaatatc tcagaaaatt tatagcaagg tacgtaacaa atatctgtac
      121 ttaaaactga atcttaattt gtaacaagcg ccggtactcc gattttgacc tgagtcaggt
      181 ctgcatttct agcacacctg ggatggcagt ttggtgtgtg tgacagaact gccacagtcc
      241 aggctggtct tgcaaaaccc tggttattgc agtctcctgg cgtttgatac cgtctgggcc
      301 gggagatttt ggatctgaat tcattgagag agctttggca cattagttgc tcttatccaa
      361 agtgcgctgg gaatgccgca gacgcgaaaa gccgggaacg ttggttcgag tcaatgcggc
      421 attcttcaac tttttaccag ccttattcat acaaaaaaag atacaaacta aagtttgagg
      481 tcattccatg atgaagacag gggaactccg gcctattttc tttgatcctg accaccgccg
      541 ctggaactgg tttcggtgga cgatcaaact tgtcgggtac acgctgggcg gtttactggc
      601 tgtgctgggc atcagttttt tgattgatcc ggtgttaccc caattgaccc tggcccaatt
      661 caaactggca aattcaggtc accatccagt caagcctgaa ccgattcagc ccgagatcgg
      721 aagccagaag cataaagcca gggttggtga gtcgcgaacc gcctctccac acccggaacc
      781 gtccccgtca caacctgtct ccctgtccca ggccagtcaa tcaaatgaag tgattggatt
      841 tctggtgaac tgggacgata cgagttttac ttcgctcaag caaaattccg gtcatttgac
      901 caaactcatt ccagaatggc tccatctggc ggacgccgac ggcaacatca cgcaggatga
      961 acctcaaaaa caacagcagg ttctggcttt tttgcgcagc caccagccac aaatcaaagt
     1021 tgttccgctg at
//
LOCUS       DAOQDO010002531         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_205560_length_1030_cov_2.976410, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002531 DAOQDO010000000
VERSION     DAOQDO010002531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205560_length_1030_cov_2.976410"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKZ53_28495"
     CDS             <1..504
                     /locus_tag="PKZ53_28495"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNJ44454.1"
                     /translation="DPHSPKDSFFYGHMGWMLVRSSLSRHRILARDLASDRFVNWLDR
                     GMGGFSPLLPWLLTMIICFAVDGWAGVIWGGVLRTLWVWHATWCVNSVCHRWGSRPHD
                     TRENSGNVWWVGLWALGEGWHNNHHAHPKAAIHNYHWWEIDPSAYLILGMEKIGLVRK
                     IVQSNKA"
     gene            complement(731..>1030)
                     /locus_tag="PKZ53_28500"
     CDS             complement(731..>1030)
                     /locus_tag="PKZ53_28500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44455.1"
                     /translation="DPLSRTVTQGQSTSYDVTITRVNYTGTVSLIISGEIPPGTQIAP
                     SPTGFQDRGLLQVRTSADTPVGEYQLFLNGLADGVGIQSIPFRLIVEAPSGVPRP"
BASE COUNT          224 a          268 c          278 g          260 t
ORIGIN      
        1 gatccgcatt cacccaagga cagcttcttc tatggccata tgggctggat gctggtgcgt
       61 tcgtccctgt cgcgtcatcg gattctggct cgggatctgg cctcagaccg atttgtcaac
      121 tggcttgacc ggggcatggg tggatttagc ccgcttttgc cctggctgct caccatgatc
      181 atctgctttg cggttgatgg ttgggccggg gtcatttggg gcggagtgtt acgcacgctt
      241 tgggtctggc atgctacctg gtgtgtcaac agtgtctgcc accgctgggg cagccggcca
      301 cacgataccc gtgagaatag cggaaatgtc tggtgggttg gcctctgggc gcttggcgaa
      361 ggctggcaca ataaccacca tgcccatcca aaagcagcga ttcataacta ccactggtgg
      421 gaaattgacc cttcagccta cttgattctg ggaatggaaa agattggatt ggttcgaaaa
      481 atcgtgcaat ccaataaagc ctgaccagtt cacacagtat ttatcaagaa aagccacccg
      541 aattcaggtt caaccagatt cgggtggctt ttcttttttc ggaaggcaga cggagctgaa
      601 gacttcgggc taaagacttc ggatttaacc tgttgttttc aacccaacat cttcagccca
      661 atgtcttcag cccgttgtct tcaaccccga gcctgcgagt cttcagccca atgtcttcag
      721 cccttgccgt ttaaggccgt ggcacaccag atggcgcttc aacaatcaag cggaatggaa
      781 ttgactggat ccccacgcca tcggccagac cgttgaggaa cagttgatac tctcccacgg
      841 gagtgtcggc actggttcta acctgtaaca ggccacggtc ttgaaaacca gttgggcttg
      901 gggcaatttg ggttcctggg ggaatctcac ctgaaatgat caaggaaaca gttccggtat
      961 aattgaccct ggtaatcgtc acgtcataac tggtgctttg gccctgagta accgttcgac
     1021 ttaacgggtc
//
LOCUS       DAOQDO010002532         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_206011_length_1029_cov_3.107803, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002532 DAOQDO010000000
VERSION     DAOQDO010002532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206011_length_1029_cov_3.107803"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..856)
                     /locus_tag="PKZ53_28505"
     CDS             complement(152..856)
                     /locus_tag="PKZ53_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ44456.1"
                     /translation="MSEATKSLIRLDNITKVFYTDEVETHALSGVHLDINKGEYVSIA
                     GPSGCGKSTLLSILGLLDSPTDGGYWLNDKPVESLKLSERARIRNREIGFIFQSFNLI
                     GDLTVYENVELPLTYRGMSSAERKKRVHEALDKVGMSHRAKHLPSQLSGGQQQRVAVA
                     RAIAGDPLLLLADEPTGNLDSKNGEAVMDLLRELHRGGATICMVTHDPRFSRYADRTI
                     HLFDGRVVEEEQVEAA"
BASE COUNT          233 a          261 c          274 g          261 t
ORIGIN      
        1 gaggaccgtg gtggcgttgc ttgggaagta atatcagtgg ggcttggggc tgagggcttg
       61 gggctgaaga aagcgggctg aagaaagtgg gttcagggga ttgaacccac tttcttcagc
      121 cccgagcctg cgagtcttca gcctattttc ttcatgcggc ttcaacctgt tcttcttcga
      181 ccacgcgacc gtcaaagaga tggatcgttc ggtcggcata gcgtgaaaac cgtgggtcgt
      241 gggtcaccat acagatcgtt gcgccacccc gatgcagttc acgaagcaaa tccatcacgg
      301 cttcgccgtt tttcgagtcg aggtttccgg ttggttcatc cgcgagcagg agcaaaggat
      361 ctccggcaat cgctcgggcc acggcaacac gttgctgttg accgccggaa agctggctgg
      421 gcaaatgctt ggcccggtgt gacatcccca ctttgtcgag cgcctcgtga acccgttttt
      481 tacgctcggc ggaagacatg ccgcgatagg tcaacggcaa ttcgacgttt tcatacacgg
      541 ttaaatcgcc aatcaaatta aaactctgga agataaagcc aatttcacgg ttgcgaatcc
      601 gggcgcgctc tgaaagtttc aagctttcga ctggcttatc attcaaccag tagccgccat
      661 cagtcggaga gtcaagcaat cctaaaattg acagcaaggt tgacttccca catccggagg
      721 gcccggcaat cgagacatat tcgcctttgt tgatgtccag atggacacct gaaagcgcgt
      781 gggtttcaac ttcgtcggtg taaaagacct tggtgatgtt atctaaccgg atcaatgatt
      841 tcgtggcttc tgacattgtg tttccccttc tcgatatttt cattctatgg tcagttgtga
      901 cttgcgcttg acgacaatca atttagttga attcgatcaa cggcatccca ggccgacata
      961 tctgaaagaa tgacctgttc accaggctta agcccgtcaa cgacttcgat ggtgttgact
     1021 gaacttcgc
//
LOCUS       DAOQDO010002533         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_207250_length_1026_cov_2.993821, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002533 DAOQDO010000000
VERSION     DAOQDO010002533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207250_length_1026_cov_2.993821"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PKZ53_28510"
     CDS             complement(<1..953)
                     /locus_tag="PKZ53_28510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ44457.1"
                     /translation="MRRTTEADFRQLFEHSQDGMYFMFFDEGTGIPWEATASDEQKKL
                     WIEHTLNHLRMTEVNEVMATMFGFSRPDELYGVAIKHFFADSDTVGEQLTRFFDQGNM
                     TATTKEKTRTGEILWIEGHHLCLYENDRLVGVCGIQRDITQRHEREAEIARRTEELHT
                     LQSMSTILSTTLDANVIMQLILERAVNLLGADGGSLCRYNWEKDEELVALAIGIDKPV
                     EGLRIPISESESGRVAQKRRPGIFPWEHHHREGYKPAIELLPPKHTIMSPMIARDRVV
                     GSLILSRRLNRPPFVERDLSLLSTLAEQMAMALDNAELYEAV"
BASE COUNT          239 a          265 c          262 g          260 t
ORIGIN      
        1 acggcttcat ataattcagc attatcaagc gccattgcca tttgctcagc cagggtggac
       61 agcaacgaca gatcacgttc gacaaatggt gggcggttga gtcggcggct taaaatcaat
      121 gagcccacca cccggtcgcg ggcaatcatc ggcgacataa tcgtatgttt cggaggcagc
      181 agttcaatgg caggtttata gccttcgcgg tggtgatgtt cccaggggaa aattccaggc
      241 cggcgttttt gggccacgcg tccactttca ctttctgaga ttggaatcct taacccttcg
      301 actggtttat caattccaat tgccagcgcc accagctctt catctttttc ccagttgtag
      361 cggcacagcg acccgccatc cgccccgagc aggttgaccg ctcgttccag aatcagttgc
      421 atgatgacgt tggcatccag tgtagtgctc aaaatcgtgc tcattgactg cagggtgtga
      481 agttcttcgg tccggcgggc aatttccgct tcgcgttcgt ggcgttgggt gatgtcacgc
      541 tggataccgc aaacccccac cagccggtcg ttctcataca ggcacaggtg atgaccttcg
      601 atccagagga tctcacccgt tcgggtcttt tccttggtcg tggccgtcat gttgccctga
      661 tcaaaaaacc gcgtcagttg ctccccaacc gtatcgctat cggcaaagaa gtgtttaatg
      721 gcgacgccat acaactcatc tggtcgtgaa aagccgaaca ttgtcgccat cacctcatta
      781 acttcagtca ttcgtaaatg attcagggtg tgttcgatcc agagtttctt ttgctcatcc
      841 gaggcggttg cttcccaggg aatgcctgtt ccttcatcaa aaaacatgaa gtacatccca
      901 tcctgcgaat gttcaaaaag ctggcgaaaa tcggcttcgg tggtacgacg cataggagga
      961 atgaagaatg aagaatgaag aattgagaat gaagaatgaa gaaaatacct ggtgcttggt
     1021 gcttgg
//
LOCUS       DAOQDO010002534         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_207271_length_1026_cov_2.744593, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002534 DAOQDO010000000
VERSION     DAOQDO010002534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207271_length_1026_cov_2.744593"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1026)
                     /locus_tag="PKZ53_28515"
     CDS             complement(<1..>1026)
                     /locus_tag="PKZ53_28515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44458.1"
                     /translation="TSVLRASIDHSHNVVLEQANRYGHALLVGKTTSHGTVSECLLRE
                     VAILKAQVETYKTKLEEFGFDFVGTRERTEAGQEFWNGGKYRDIFTSLTTGNFISSGL
                     VTHDELERLYFELIPNPIDQFLSLKAEVEKTLNREKGARTIFQFVLDQPDDVKRLLFE
                     LGRTRFRKVQETSIAEKLEKLPQKEVDHRIEEAFRRSHPLLRIDRSALFANDAKTGSR
                     ITGHNPGFHNIRKMATCRVQDDLYLDVTQMGENRSKLAVAFQTKVQGTDSSQGLPDRY
                     RIVFTQEKGVFPLYCLADLPQLRKAYLFEMRQDNARPRETNRKVVFPDIFPPDPREVG
                     MPRRVKKA"
BASE COUNT          217 a          268 c          283 g          258 t
ORIGIN      
        1 gcttttttaa cccggcgggg catcccgacc tcgcgtggat caggcgggaa aatatctggg
       61 aagacgactt tccggttggt ttcacgtggg cgggcgttgt cctgccgcat ttcaaagagg
      121 tatgccttgc gtagttgcgg caggtctgcc agacaataga gtggaaaaac gcctttctcc
      181 tgggtgaaga caattcggta gcgatccggc aacccctggc ttgagtctgt tccctgaacc
      241 ttggtctgga atgcaaccgc cagtttggaa cggttttcac ccatctgggt gacatccaga
      301 tacaaatcgt cctgaacccg gcaggtcgcc attttgcgga tgttgtggaa tccaggattg
      361 tggccggtga tccggctgcc ggttttggca tcgttggcaa acaaggcgct tcggtcaatt
      421 cggagcaagg gatgtgaccg ccggaaggct tcttcaatcc ggtgatcaac ttccttttgg
      481 ggtaatttct caagtttttc agcaattgag gtttcctgga ctttccggaa gcgcgttcgg
      541 cccagttcaa acagcagacg tttgacatca tccggctgat ccagcacaaa ctgaaagatc
      601 gtccgggcac ccttctcccg attcaatgtc ttttcaactt cagctttgag ggacagaaac
      661 tgatcaattg ggttcggaat cagctcaaag tacagccgtt cgagttcatc gtgggtgacc
      721 agcccgcttg aaatgaaatt cccggtggtc aggctggtaa aaatatcgcg gtacttgccg
      781 ccgttccaga attcctgccc ggcttcggtg cgttcccgcg tgccgacaaa atcgaatccg
      841 aattcctcaa gcttagtttt gtaagtttca acctgagctt tgaggatggc aacttcacgc
      901 aacagacatt cgctgaccgt gccatgcgaa gtggtcttgc cgaccaggag cgcgtgccca
      961 taacggttgg cctgctccag cacgacgtta tggctgtggt caatcgaggc ccgcagtaca
     1021 ctggtg
//
LOCUS       DAOQDO010002535         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_207675_length_1025_cov_2.861856, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002535 DAOQDO010000000
VERSION     DAOQDO010002535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207675_length_1025_cov_2.861856"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1025)
                     /locus_tag="PKZ53_28520"
     CDS             complement(54..>1025)
                     /locus_tag="PKZ53_28520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44459.1"
                     /translation="RAYPWQPTSTMYGVGQIEVSEFASSLQFSVAQAAKPSVIPPWAA
                     PPGEKTPEAVEEAEDWEEDPEFEENSEAFPAGQLPAKVAQPVATDDAVYLPLAGVDHF
                     SILAQLKYGQGNFICLSDEFIFANNGISKADNAILGLNLASAVLDAVQKKYPASKHQI
                     LFDEFHHGYHNGGPNYATLAGYFRGSPVPWLMWQFAFLGLFIVFTYGRRFARPVPLKR
                     VNRASSLEYVSAMAQIQLVAKSYDLAIENIYNRFHRVLCRYGGVATNVKIDRIATAVA
                     SRGQVDANELIRVLQRCQEVLNGKAITEKELLHLTQKIREIEHKIGS"
BASE COUNT          248 a          260 c          268 g          249 t
ORIGIN      
        1 ggctgcccac cggctacttt ccagcgccct ccgggcggaa gagtcctggc ttttcagctt
       61 ccaatcttat gttcaatttc ccggattttt tgagtgaggt gcagcagttc tttttcggta
      121 atggctttgc cgttgaggac ttcctgacag cgttggagga cccggatgag ttcgttggca
      181 tcaacctgtc cccgcgaagc aactgccgtg gcaattcgat caattttgac attggtcgca
      241 acaccgccat agcgacacaa gacccgatgg aagcggttat agatattctc aatggccagg
      301 tcatagcttt tggcgacgag ctggatttgt gccatggctg aaacatactc aagcgaactg
      361 gcccgattga cccgtttgag cggcaccggg cgagcaaatc gccggccata ggtaaacaca
      421 ataaacagcc ctaaaaatgc aaactgccac atcagccagg gaactggcga accacggaaa
      481 tacccggcca acgtggcata gtttgggccg ccgttgtgat agccgtgatg aaattcgtca
      541 aacagaatct gatgtttgga agccgggtac tttttctgaa cagcatcaag tacagcactc
      601 gccaggttga gaccaagaat ggcattgtcc gcttttgaaa tgccattgtt tgcaaaaatg
      661 aattcatctg acagacatat gaagtttccc tgaccatact ttaactgagc cagaattgaa
      721 aaatggtcaa cgccagctaa gggtaagtac acggcatcgt cggtcgctac gggttgagcc
      781 actttggctg gaagttgccc ggctggaaat gcttcggaat tctcctcaaa ctcaggatct
      841 tcttcccaat cttcggcttc ttcaacagcc tcgggggttt tttctccagg tggggctgcc
      901 cagggtggaa tgaccgacgg tttggcggcc tgcgcgacag aaaattgcag actgctggca
      961 aactctgaga cttcaatttg accaacacca tacatggtgc tggttggttg ccagggatag
     1021 gcccg
//
LOCUS       DAOQDO010002536         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_207871_length_1025_cov_1.769072, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002536 DAOQDO010000000
VERSION     DAOQDO010002536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207871_length_1025_cov_1.769072"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..>1025)
                     /locus_tag="PKZ53_28525"
     CDS             complement(380..>1025)
                     /locus_tag="PKZ53_28525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44460.1"
                     /translation="IGELIPADWQLIGDPSIRRETEHVTALVAQIPDTEHKEELCDTF
                     ARQTGYTLEISVAEGKARLVSRPVGTDGTASLQPVMELNQAYQHIKHHFEQEPNQPLK
                     VGMKSDVTGKYIEVAFLSAIVGEWYLEDLTKLQEETGWEIRLKPTTDEYKIKELARQM
                     CARWKIVRGPGLTPGATEVKVWVSDILSAEEEKELIDEFYYQTGFELYVDIKGA"
BASE COUNT          223 a          258 c          277 g          267 t
ORIGIN      
        1 ggaggtgatt gacctgattg atcagttgaa aaaggctggt gccacaactg ttgatttgga
       61 actggcagaa aaccaaagca ggggtcaggg ttcggaggga aactcttgct ggtaaaggtg
      121 gttgggggca taaggggaag accggaagat acaatctggc tcataccggt gtgcaatgaa
      181 atgctcgtat tcgaagacat ctgaaagttg cgaattgacg gccactacag atatatcctg
      241 gattacaatc acctcaggca gttcaggagt tgggaatggc gtggcagggc tattacacaa
      301 cgacccttct tcaacccgaa gttttcagcc cgaagtcttc agccccaggc ccagagtctc
      361 cagcccaagg tcttcagccc taagctcctt tgatatcaac atataactca aacccggtct
      421 ggtagtaaaa ctcgtcaatc aattcttttt cttcttccgc cgataaaata tcggagaccc
      481 agactttgac ttcagttgcg ccgggggtga gacctgggcc gcgcacgatt ttccatctgg
      541 cgcacatctg gcgggcaagt tctttgattt tgtattcatc cgtcgttggt ttgagccgga
      601 tttcccaacc ggtttcttcc tggagtttgg tcagatcctc cagataccac tcgccaacaa
      661 tggcggagag aaacgccact tcgatgtatt ttcccgtgac gtccgacttc attccgactt
      721 tgagcggctg gtttggttcc tgctcgaaat ggtgtttgat gtgctgatat gcctgattga
      781 gttccatcac tggctgcaac gaggctgtcc cgtcagtgcc gactggacgc gataccagcc
      841 gcgctttgcc ttctgccaca ctgatttcca gcgtgtagcc cgtctgccgc gcaaatgtgt
      901 cgcagagttc ttctttgtgt tcggtgtcgg gaatctgggc gacgagagct gtgacgtgtt
      961 cggtctcgcg ccggatcgaa ggatctccga tcaattgcca gtctgccggg atgagttcgc
     1021 cgatg
//
LOCUS       DAOQDO010002537         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_208326_length_1024_cov_1.650155, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002537 DAOQDO010000000
VERSION     DAOQDO010002537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208326_length_1024_cov_1.650155"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..>1024)
                     /locus_tag="PKZ53_28530"
     CDS             complement(187..>1024)
                     /locus_tag="PKZ53_28530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44461.1"
                     /translation="NQELGDIISTGNNAFSSYHGMTLGVDVFNLKGVNLTARYTYSKA
                     LDNLSSTLSESSNNRNLGLLDPFHPELDRGPADFDTRQRFIVSGIWELPFGRAFRGAK
                     KHLVDGWQVISVFSSQTGTPFTLFDSTGAILYAPRLTEIGSLDRNGSGNPAAVPGEFN
                     SFNYLDLSSQLAGAGANGANGAGNFSDFGFDGKGTYTAGMTGRNAFRGPGQYRLDTSI
                     AKRFDLTEKMKVQVRFEFFNVLNHANLFTVLDQTDIALQPYVPARRDGRRQVQLAVKL
                     IF"
BASE COUNT          260 a          253 c          279 g          232 t
ORIGIN      
        1 ggtcgcgctg gaaacatcct gattgacggt agcgtccgat tcaggtgatt tttgtttgag
       61 acgcaacgcc cccactacga gctgggtgcc acagtggggg caaaactttg acgtctcgga
      121 agtgagaaaa ccacatcctg ggcagggcat aagcgattcc cttgaggttc agtacagcct
      181 ggaacgttag aaaatcagtt tgaccgccag ttggacctga cggcgtccat cacgacgcgc
      241 tggaacatag ggctgtaagg caatgtcggt ttggtccagc acggtaaaca ggttggcatg
      301 attgagcaca ttgaaaaatt caaaccggac ctgcactttc atcttttcag tcaggtcaaa
      361 ccgtttggca atactggtat caagccggta ttggcctggc ccgcgaaagg cattacgccc
      421 ggtcatgcct gcggtatagg ttcctttgcc gtcaaatcca aaatcggaaa aattgccggc
      481 gccgttagcc ccattggcgc cagcacccgc aagctggctg ctcaggtcaa gatagttaaa
      541 tgaattaaat tcgcctggaa cggctgccgg attccccgac ccgttgcggt cgagcgagcc
      601 aatttcggtc aatctgggcg cgtacaaaat tgccccggtg ctatcaaaca gagtgaacgg
      661 ggtgccggtc tggcttgaaa aaacggaaat cacttgccag ccgtcaacga ggtgtttttt
      721 ggcaccacga aaagcacggc caaacggaag ttcccaaatt ccactcacaa tgaatcgctg
      781 ccgggtatca aaatcggctg ggccgcggtc aagttcagga tgaaagggat ccaacaaacc
      841 cagatttcgg ttgtttgagg actcgctcaa ggttgaactg aggttatcaa gtgctttgga
      901 gtaggtgtag cgggcggtga ggttgacccc tttgagatta aacacatcaa ccccaagggt
      961 cattccatgg taggaagaaa aggcattgtt cccggtgcta ataatatctc caagctcttg
     1021 attt
//
LOCUS       DAOQDO010002538         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_208470_length_1023_cov_3.279959, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002538 DAOQDO010000000
VERSION     DAOQDO010002538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208470_length_1023_cov_3.279959"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..598
                     /locus_tag="PKZ53_28535"
     CDS             110..598
                     /locus_tag="PKZ53_28535"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNJ44462.1"
                     /translation="MLFLRSLFFTILFPGTVTVGIPYWLVSSVPVIHRPNPGPLHFFG
                     LPLMVVGGIGLLRCIWQFFAEGRGTLAPVDPPKHLVVHGLYRLVRNPMYVCVLLVLLG
                     EAIFFQSQAILIEAGVFFLGTHLFITLYEEPALRKQFGTSYENYVQKVGRWVPRLPDN
                     QP"
     gene            753..>1023
                     /locus_tag="PKZ53_28540"
     CDS             753..>1023
                     /locus_tag="PKZ53_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNJ44463.1"
                     /translation="MTDVAKAMATQLANIEKRSGKSLAELAQIIQASGLSKHGEIRDM
                     LKRDLGLGHGDANTLVHHVLKSDGQSAAAEQNLSTDDVVSGLYTGP"
BASE COUNT          230 a          267 c          240 g          286 t
ORIGIN      
        1 cctgagccca atgtcttcaa ctccttttct tccactctgg ttttagggtt caagggtggg
       61 agttcttcat tctcaattct tcattctgca ttctcaattg gtacagccca tgcttttcct
      121 gcgaagcctt ttcttcacaa ttttatttcc aggaacagtc acggtgggca ttccctactg
      181 gctggtttca tcggttcccg tgatacacag accgaatcct gggccacttc attttttcgg
      241 gttgccactg atggttgtgg gagggatcgg cctgctcagg tgtatctggc agttttttgc
      301 tgaaggccgg ggcacgctgg cgccggttga tccaccgaaa catctcgtcg tccatgggct
      361 ctatcgcctg gtacgaaacc caatgtatgt gtgcgttttg ctggtcttac tgggagaagc
      421 gattttcttt caatcgcaag ccatcttgat tgaagccggt gtatttttcc tcggtactca
      481 cctttttatc acgctgtatg aagaaccagc tttgcgaaaa cagttcggta cgtcgtatga
      541 gaactatgtc caaaaggttg ggcggtgggt tccacgattg ccagacaacc aaccttgaat
      601 tgcctgaccg ggttgttttg ggttctctga atgccccaaa atcatttcct tgaaataagt
      661 accgggtaat ttacaatacg gcggctgttt atttccaaat caccctctca tttctgaact
      721 atctcaaccg atttaaaatc aaggagatcc atatgaccga cgtcgcgaag gccatggcca
      781 cccagcttgc caatatcgaa aaacgaagtg gaaagtcact cgctgaactg gcccaaatca
      841 tccaggccag cgggttgtca aaacatggtg aaatccgcga tatgctcaaa cgtgaccttg
      901 ggttgggaca cggagacgcc aacacgctgg tccatcacgt cctcaagtca gacggtcaaa
      961 gcgcggccgc cgaacaaaat ctttccactg atgacgtcgt ttccgggctt tacaccggtc
     1021 cca
//
LOCUS       DAOQDO010002539         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_209324_length_1021_cov_2.692547, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002539 DAOQDO010000000
VERSION     DAOQDO010002539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209324_length_1021_cov_2.692547"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PKZ53_28545"
     CDS             complement(<1..996)
                     /locus_tag="PKZ53_28545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44464.1"
                     /translation="MYESTRAYHPIVQGQLPKEVAAPDQPFVADLGNGISVSIPISVF
                     ATGKKTLPLKAPGASQPIVLERFTGDDRATRLADIALARNTFQHFYPYFDVVKTDWPA
                     VLPESLKEAAIAPDGQAFLLTLQRMVAQLQDGHGFVSQGGLSQGVLPFEWDWAENRLV
                     VTEVAASIADKIKRGDVVESVNGLSTEKAIFEKETLISGATPQWKRFIALRRLQSGQI
                     KTEVKLDLRRADNSTYQITLPFGVPPEGLEETRPPKLHELKPGIFYIDVERITDDDFQ
                     QALPQLIQAKGVIFDFRGYPNCSPMVIQHLTKTPIESARFMVPLVSTPDHQNMINF"
BASE COUNT          244 a          254 c          256 g          267 t
ORIGIN      
        1 aaaattgatc atgttttggt gatcaggtgt agataccaac gggaccataa accgggctga
       61 ttcaatcggt gttttcgtta aatgctggat caccatgggc gaacagtttg gatacccccg
      121 gaagtcaaag ataacccctt tggcttgtat gagttgtggg agggcttgct ggaaatcatc
      181 atcggtgatg cgttcaacat caatgtagaa gattccaggt ttgagttcgt gcaattttgg
      241 agggcgcgtt tcctctaatc cttcgggtgg aacgccgaaa ggaagcgtta tttgataggt
      301 cgagttatct gcccggcgaa ggtcaagttt cacttcagtc ttgatctgtc cggattgcaa
      361 ccgtcgcagg gcaataaagc gtttccattg aggtgtagcg cctgaaatca atgtttcctt
      421 ctcgaaaatg gctttttcgg tggagaggcc gttcactgat tccaccacat caccccgctt
      481 gattttgtcc gcaattgagg ctgccacttc agtaaccacc agacgatttt cagcccagtc
      541 ccactcaaag ggaagtaccc cctgtgacag ccctccctgg ctcacgaacc cgtgcccatc
      601 ctgaagctga gccaccattc gttgaagggt gagtaaaaat gcctgaccat ctggggcaat
      661 cgccgcctct ttcaacgatt ctggcaagac tgccggccag tcggttttga ccacatcaaa
      721 ataaggataa aaatgctgaa acgtattccg ggccagggcg atatcagcca atcgagttgc
      781 ccggtcatca ccggtaaatc gttccagtac aattggttgt gaagcaccag gtgctttcaa
      841 agggagtgtt tttttcccgg ttgcaaagac actgattggg atcgaaaccg aaataccgtt
      901 tcctaaatca gccacaaacg gctgatctgg cgccgccacc tctttgggga gttgtccctg
      961 gacaatcgga tgataggccc gggtgctttc atacactcca ttggagtctc cacctttttt
     1021 g
//
LOCUS       DAOQDO010002540         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_210614_length_1018_cov_2.541018, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002540 DAOQDO010000000
VERSION     DAOQDO010002540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210614_length_1018_cov_2.541018"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1018
                     /locus_tag="PKZ53_28550"
     CDS             <1..>1018
                     /locus_tag="PKZ53_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ44465.1"
                     /translation="TSTSPVETFAEPCTGITPVMYKAPNLRTQQEIAVLNIGTPTFMR
                     APGENPGLFALESAMDELAWALKMDPIELRLKNETTEHQQRKMPFSSKFYADCLKVGA
                     EHFGWKDRPKVPRSLTKDGKLIGWGVAGSTFPGLRMPATVKVRLLANGTAEVLTAGND
                     MGTGAYTVVAITAAETLGLPVEKIHVQMGDSRLPDGGLAGGSQMTATLAPAVTAACQA
                     VLKQAKASSASEAFDGLQKSGRGAVEATATSSPGAEMRKWAFQSWGAHFCEVAVDEEI
                     GRLRVTRWHAVMNIGQVINSKAAASQVRGAVIMGIGQALMEECVFDPNLGNPVVYDLA
                     TYHFP"
BASE COUNT          240 a          286 c          284 g          208 t
ORIGIN      
        1 aaacctcgac gtcgccagtt gaaacctttg ccgaaccctg caccggcatc acaccggtga
       61 tgtacaaagc acccaatctg cgaacccagc aggaaattgc ggttctcaac atcggcacgc
      121 cgacgtttat gcgcgctccg ggtgaaaatc caggcctgtt tgcgctggaa tccgccatgg
      181 acgaactggc ctgggcgctc aagatggatc caatcgaatt gcgcctcaag aacgaaacca
      241 ccgagcatca acagcgaaaa atgccttttt cttcaaagtt ttatgctgac tgtctcaaag
      301 tcggtgccga gcattttggc tggaaagacc gccccaaagt acctcggtca ctcaccaaag
      361 acggaaaact gattggctgg ggtgtggctg gctcaacttt ccccggactc cgaatgcccg
      421 ccacggtcaa agtccgcctg ctggccaatg gaacggctga agttttgacc gccggcaacg
      481 atatgggcac cggggcctac acggtcgtcg ctatcacagc ggctgaaacg cttggtctcc
      541 cagttgagaa aatccacgtc cagatgggcg attcgcgcct tccggatggt ggactggctg
      601 gaggttccca aatgacagcc accctggcgc cagccgtaac tgccgcctgt caggccgtgt
      661 tgaaacaggc caaggccagt tcagcttctg aagcctttga cggactccaa aaatcaggcc
      721 gtggtgccgt tgaagcgacg gcaacctctt cacccggcgc ggaaatgcgg aaatgggcat
      781 tccaatcgtg gggagcacat ttctgtgaag tcgcagtgga tgaagaaatt ggtcggttga
      841 gagtcacccg ctggcacgcg gtgatgaata tcggtcaggt tatcaacagc aaagcggctg
      901 ccagtcaggt ccgtggggca gtaattatgg gaattggtca ggcgctcatg gaagaatgcg
      961 tctttgatcc aaaccttgga aatcccgtgg tctacgatct ggcgacgtat cacttccc
//
LOCUS       DAOQDO010002541         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_210692_length_1018_cov_2.169263, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002541 DAOQDO010000000
VERSION     DAOQDO010002541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210692_length_1018_cov_2.169263"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKZ53_28555"
     CDS             <1..510
                     /locus_tag="PKZ53_28555"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNJ44466.1"
                     /translation="VDYYFAKAAADEAKQYLADQEKQKMQLDVQKKELEQYEQKNKII
                     QQRLVVIKQLAEGGNQGPVKILNAVNTKMPPDGIILGQIAQKGAKITIEGVTTNRERV
                     TEFAKDLEIEGGGLFTNVSVLIDAPGKEDATEEQNIKFTIDCVYNPTPPPAAGNAAAT
                     TAASPPAQK"
     gene            604..>1018
                     /gene="pilO"
                     /locus_tag="PKZ53_28560"
     CDS             604..>1018
                     /gene="pilO"
                     /locus_tag="PKZ53_28560"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNJ44467.1"
                     /translation="MAEGGGGIGFLANIPWWGQLLILLVLVLGLSYLGDVFLCSDLRV
                     NAEKDRKKAEDLKKENEKTKQFIAQYAQYKAAYEQALQDLKSYRERLPESPLIPNTVE
                     TIYQQAEKVGLVVRGIKPKTETKKDFYKEVPLEVNL"
BASE COUNT          319 a          200 c          261 g          238 t
ORIGIN      
        1 gtggattatt actttgcaaa agctgctgcc gatgaagcca agcagtattt agctgaccag
       61 gaaaagcaaa agatgcagct tgatgttcaa aagaaagagc tggagcaata tgagcaaaag
      121 aataaaatta tccagcagcg cctggttgtg atcaagcaac tggccgaagg cgggaaccag
      181 ggaccagtga agatcttaaa tgctgtcaat acaaaaatgc ctcctgatgg aatcattttg
      241 ggccaaattg ctcaaaaagg agccaagatt accattgaag gtgtgactac caatcgtgag
      301 cgagtaacag agtttgcaaa agaccttgaa attgaagggg gagggctctt cacgaatgtg
      361 tcggttctaa ttgatgcacc cggaaaagaa gacgccacag aagaacagaa catcaagttt
      421 acgattgatt gtgtttacaa tccaacgccg ccaccggcgg ctggaaatgc tgccgcgact
      481 accgccgctt ctcccccagc tcaaaagtga gacgtttttg gagctggctt acatggactg
      541 gcgcgcagtg gcacgactgc aaatcatcaa catcaacact gagtttttga aggagtcatt
      601 gtcatggctg aaggtggtgg cggaatcggt tttctggcaa atattccctg gtggggacaa
      661 ttgctcattc tcctggtact ggtgcttgga ttaagttatt taggggatgt ttttttgtgc
      721 tcggatcttc gggtgaatgc agaaaaagat cgaaaaaaag ctgaagactt gaaaaaagag
      781 aatgagaaaa caaaacaatt cattgctcaa tatgctcaat acaaagcggc ttatgagcag
      841 gcattacagg atctcaagag ttatcgggaa cggcttccag aatccccatt aattccaaac
      901 acggtggaaa ccatctacca gcaggctgaa aaagttggtt tggtggtacg cgggattaag
      961 ccaaagacgg agaccaagaa ggatttttat aaagaagttc ctttggaggt caaccttg
//
LOCUS       DAOQDO010002542         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_211041_length_1017_cov_2.723493, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002542 DAOQDO010000000
VERSION     DAOQDO010002542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211041_length_1017_cov_2.723493"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..878)
                     /locus_tag="PKZ53_28565"
     CDS             complement(132..878)
                     /locus_tag="PKZ53_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ44468.1"
                     /translation="MRISASTAWVIRPRPIPNATRRLFCFPYAGGGATVFHAWARLLP
                     PHVELCNVQLPGRGSRLGEAPFTSLPPLIEAVASALTPFFDRPFIFLGHSMGGLISFE
                     LTRYLRRFQHPMPERLVISGRGAPHLPDLKKPLHALPETEFLHELRQLNGTPREVLDN
                     RELMELCLPILRADFAVCETYQYRPESPLDCPLTVLGGVEDRDIDRERLTGWRDHTTQ
                     DFQLRMFPGDHFFLHSAQAEYLQLVSALLI"
BASE COUNT          280 a          260 c          276 g          201 t
ORIGIN      
        1 aacaccgcct caaatgccgg cagcaaaggc accagactgg caatagaagc tcgccagacc
       61 tgaaccacgt tggggacaag ttcacttcgg tcacttccag gaagccattc gtccgtcatt
      121 ttaccgttcc ctcaaatcag cagcgcgctc accagttgta aatactcagc ctgggctgaa
      181 tgcaggaaaa aatgatctcc gggaaacatc cgaagctgaa agtcctgagt ggtgtgatcg
      241 cgccagccgg tcagtcgttc acgatcaata tcccgatcct caaccccacc caaaaccgtc
      301 aaggggcaat ccagtggtga ttcgggacga tactggtagg tttcacagac tgcaaaatcg
      361 gcgcgcaaaa tcggcaggca caattccatc agttcacgat tatcgagtac ttcccgtgga
      421 gtaccattca attgccgaag ttcgtgaagg aattcagttt ccggcaacgc atggagcggt
      481 tttttcaagt ctggaaggtg tggcgcacca cgccctgaga tcaccagtcg ctcaggcatc
      541 ggatgctgaa agcggcgcag gtaccgggtc agttcaaagc tgataagccc acccatactg
      601 tggccgagaa aaataaatgg gcgatcaaaa aacggagtca gcgcactggc aacggcttcg
      661 attaaaggtg gaagtgacgt aaacggcgct tcccccagac gacttccccg ccctgggagc
      721 tggacattgc ataactcgac gtgtggtgga agcaggcgtg cccaggcatg gaaaaccgtg
      781 gccccaccac ccgcataagg aaaacaaaac aatcgtcgcg tggcatttgg gattggcctg
      841 ggacggatca cccaggcagt actggctgaa attcgcatca tatggaaggt gtttcaagtt
      901 cggagatgaa gggcgaggga attatgtatc aagaacgcgg aattgagaat gaagaatgaa
      961 gctgcttctg ccggattttg tgttggagat aaaccagaag aaccgatgag tcagtac
//
LOCUS       DAOQDO010002543         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_211259_length_1017_cov_1.787942, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002543 DAOQDO010000000
VERSION     DAOQDO010002543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211259_length_1017_cov_1.787942"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKZ53_28570"
     CDS             complement(<1..308)
                     /locus_tag="PKZ53_28570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44469.1"
                     /translation="MPSEGRGGAGIAAIVGANTGGTGAAIVGANTGEGDGTDVTFWET
                     PCKIACISFASGYRSWGSCRVLQFRISISSRLVFPAYDSQFRFPVRGSVIVVGLFPVN
                     "
     gene            316..>1017
                     /locus_tag="PKZ53_28575"
     CDS             316..>1017
                     /locus_tag="PKZ53_28575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ44470.1"
                     /translation="MQSVTPQLNWFEFEVVKLEGFTDGILGFGKKPITRSTKGRARSY
                     IEDLGKRVGLEMVAIPGGTFEMGSMQYEDEHPIHRVTISPFFMGKYPVTQAQWREVAK
                     CAKVQIDLNPEPSHFKGEYLPVESVAWFECVEFCERLSRRTEKIYRLPTEAEWEYACR
                     AGTTEDYAGYLDAMAWYDKNSEGKTHPVGQKAPNEWGLYDMHGNVWEWCQDWHQGSYD
                     RAPTMMRGGSWDNGAG"
BASE COUNT          288 a          218 c          289 g          222 t
ORIGIN      
        1 ctgttgactg ggaagagccc cacaactatg actgatcccc ggactggaaa tctgaactgg
       61 gaatcgtatg ctggaaacac cagccgggaa ctgattgaaa ttctgaattg cagcacccgg
      121 caacttcccc aggatcggta tccggacgca aaggaaatgc aggcgatttt gcaaggagtt
      181 tcccagaagg tgacgtctgt accatcgcct tcgccagtgt tggctccaac gatggcggct
      241 ccggtgccac cggtgttggc accaacaatt gccgcgatac cagccccacc acggccttca
      301 ctgggcataa atcccgtgca atcggtaact ccacaattaa actggtttga atttgaagtt
      361 gtgaagcttg aagggtttac agatggaata cttggattcg gaaagaaacc aattactcgt
      421 tcaaccaaag ggcgggcacg gtcctatata gaagatttgg gtaaacgagt aggtctggaa
      481 atggtggcaa tcccaggcgg aacctttgaa atgggatcaa tgcaatatga agatgaacac
      541 ccaatccatc gagtaacgat ctctccattt tttatgggga agtatccagt gacccaggcc
      601 cagtggaggg aagtagcaaa atgtgcaaaa gtccagattg acctaaatcc tgagccttca
      661 catttcaaag gtgaatacct gcctgtagag tcagtggctt ggtttgaatg tgttgaattt
      721 tgcgagaggt tgagccgaag gacagaaaaa atataccgac tgccgactga ggcagagtgg
      781 gaatatgcgt gtcgagcggg aacaactgag gattatgcag gttatcttga tgcgatggcg
      841 tggtatgaca aaaattcaga aggcaaaacc catccagttg ggcaaaaagc accaaacgaa
      901 tgggggttgt atgacatgca tggaaatgtg tgggagtggt gccaagactg gcatcagggc
      961 agctatgata gagcgccgac aatgatgcgg ggtggttcgt gggacaacgg tgccggg
//
LOCUS       DAOQDO010002544         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_211765_length_1016_cov_1.548387, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002544 DAOQDO010000000
VERSION     DAOQDO010002544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211765_length_1016_cov_1.548387"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1016
                     /locus_tag="PKZ53_28580"
     CDS             <1..>1016
                     /locus_tag="PKZ53_28580"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ44471.1"
                     /translation="GNLFVADTENQAIYYIDFAQQQVRLLAGAPGVPGLIDGAGRSAR
                     FRRPTSLALSSDGKIVAVVDEGNNRVRLITRSGEVRTLGRAVSRQEGDESRQASSDIE
                     FDTPQSVSFDGTGNVYVVDRTGPKVVTQALGQVPQLIDLAQPGVSFQQAASVAVQGAQ
                     TFFFDAQSPTDAEAIKVVTVGAPEIATIEDVTPTLNRLPISGGTAIRIRGKNFAPESR
                     VVVGDQVTVAQVMSATEIQLALPPQKAPGRRTISIQTRGGVAQIETNLTSNALIELST
                     GQITTTVGGVSFSGNGGLATRASLLFAQTGGLALDSLGNMYVADSANHRVRRIDVETG
                     VITTV"
BASE COUNT          234 a          237 c          296 g          249 t
ORIGIN      
        1 ggaaatttat ttgtggcaga tactgaaaat caagccattt actatattga ttttgcccag
       61 cagcaggttc gtctcctggc cggcgcacca ggtgtgccag ggttaatcga tggtgctggt
      121 cgaagtgctc gttttcgacg cccaaccagt ctggcgctca gttcagacgg gaaaattgtg
      181 gcggttgttg atgagggaaa caaccgggtt cggctgataa cccgttcggg agaagtccga
      241 acacttggtc gtgctgtttc ccgtcaggaa ggtgatgaat ccaggcaggc atcctcggac
      301 attgaatttg atactccgca atcagtgagt tttgatggaa cgggaaacgt ctatgttgtg
      361 gaccgaaccg ggcccaaagt ggtgacccag gcgctcgggc aggtgcctca actgattgat
      421 ctggcacaac ctggggtttc atttcagcaa gccgctagtg tggcggttca gggggctcaa
      481 accttttttt ttgatgccca gtcacccact gacgctgaag cgattaaagt tgttacggtt
      541 ggtgcaccag aaattgcaac cattgaggat gttactccga cgttgaatcg gctcccaatt
      601 tctggaggaa ccgcgatccg aattcgaggt aaaaactttg cccctgagag ccgggtggtg
      661 gttggagatc aagtcacggt agcccaggtg atgtctgcga ctgaaattca actggcgctt
      721 cctccgcaaa aagcacctgg acggcggacg atttcaattc agacacgggg tggcgttgcc
      781 cagattgaaa ccaacctgac gtcaaatgca ttgattgagc tttcaacagg ccaaattaca
      841 acgacggtgg gtggagtctc ctttagcgga aatggtggac tggcgacccg ggccagttta
      901 ctctttgccc aaaccggtgg tttggcgctg gatagtctgg gcaatatgta tgtggcagat
      961 agtgccaatc atcgggtgcg acgcattgat gttgaaaccg gtgtcatcac gaccgt
//
LOCUS       DAOQDO010002545         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_212438_length_1014_cov_2.252346, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002545 DAOQDO010000000
VERSION     DAOQDO010002545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212438_length_1014_cov_2.252346"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKZ53_28585"
     CDS             <1..282
                     /locus_tag="PKZ53_28585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44472.1"
                     /translation="VVNFIMSTGNPFQGASVQPTQSGNSGSIAFTTSSGQAANENDIQ
                     SILNDANARALSNGRDLVPETQGNDTYVLTSSAQSLNQPVGFSMTLNHK"
     gene            292..>1014
                     /locus_tag="PKZ53_28590"
     CDS             292..>1014
                     /locus_tag="PKZ53_28590"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNJ44473.1"
                     /translation="MKQKLYRMLAILLVVMVCGGGALQAQNPGEDPGKARKLSVGPDG
                     QSEEAVIRKAVLATVRIITGNRNPTTNEFYGTSSGSGVFIGSTGLILTARHVVFQQEG
                     KGEVFPEIWCGVMSADSPYTPPNRGFRLQLVKDERQLDLALLRIPAPVNGTFPQLNLG
                     TGSELFYGSLLRVAGFPEAGGATTTVVPTTVLGIDDQLGEIKVDGALMRGASGGPAFN
                     AKGELIGIVLGVKKDTVPFFADE"
BASE COUNT          243 a          253 c          289 g          229 t
ORIGIN      
        1 gtggtgaatt tcattatgtc aaccggaaat ccatttcaag gagcatcggt acagccgact
       61 cagtctggta attcagggtc gatagccttt accacttcaa gtggccaggc ggccaacgag
      121 aatgacatcc aatcaatcct caatgatgcc aatgcccggg ccttgagcaa cggacgcgat
      181 ttggtgcctg aaacccaggg gaatgatacc tatgtgctga ccagttccgc ccagtcactc
      241 aaccagccag ttggcttttc aatgaccctc aaccacaaat aggtgttgct catgaaacag
      301 aaactgtatc gaatgctggc gattcttctg gtggtgatgg tctgtggagg aggagcgctg
      361 caggcacaaa atccaggtga agacccaggg aaggcgcgga aactttccgt agggccggat
      421 ggtcaatcgg aagaagcagt aattcgcaaa gctgttctgg caaccgtccg catcattact
      481 ggaaaccgca acccaaccac caatgaattt tatggaacat cgtctggatc cggggtcttt
      541 atcggcagta ccgggctgat cctgactgcc cgccatgtgg ttttccagca ggaggggaag
      601 ggagaggttt tccctgaaat ctggtgtggg gtgatgtcgg cagactctcc ttacaccccc
      661 cccaatcgtg ggttccggtt gcaactggta aaagacgagc ggcagcttga cctggcactg
      721 ttacgcattc cggcgccggt gaatggaacg ttccctcagt taaatctggg aactgggagt
      781 gaactctttt acggttcgct gctcagggtg gctggttttc cagaagcagg cggggcaacc
      841 accaccgtcg tgccgaccac ggttttagga attgacgatc aacttggaga gatcaaagtt
      901 gacggtgcct tgatgcgggg tgcctcaggc ggaccggctt tcaacgccaa aggggaactc
      961 attggaattg tgctgggagt gaaaaaagac actgtcccgt tctttgccga tgag
//
LOCUS       DAOQDO010002546         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_212785_length_1013_cov_2.675365, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002546 DAOQDO010000000
VERSION     DAOQDO010002546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212785_length_1013_cov_2.675365"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..>1013
                     /locus_tag="PKZ53_28595"
     CDS             110..>1013
                     /locus_tag="PKZ53_28595"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNJ44474.1"
                     /translation="MKVARIYDFEDIRIEDMPMPEVGPTEALVQVKACGVCSGDVTPW
                     YIKRKAPIVLGHEPAGVITAVGRDVTKFKVGDRVFAHHHAPCFACHQCQKGNYVQCPT
                     WKASKIIPGGIAEYFLVPETNLGDTLRLPDSISFEDGALTEPAACSVKSIKRANLHPG
                     DTVLIIGLGIMGQMNVLLARHYGAGKIIGADLVDYRCERAIEFGADATINPTREDLVE
                     RLSELTGGKMADVVIVGPGSAPVMDLGIRCAGKGSTVVFFMGTPPEETLTVHPFHLYF
                     NEISLVSSYSCGPDDTQEALNLIEA"
BASE COUNT          228 a          223 c          286 g          276 t
ORIGIN      
        1 cgaatacaga gagccagttt gagtcctctg aacttgccag ggttatttct gcatcacctt
       61 tcttctttta ctcagaacat agtttggttg attggaaatt tgaaaattta tgaaagttgc
      121 gcgcatttac gattttgaag atatccgcat cgaggacatg ccgatgccgg aggttggtcc
      181 gaccgaagcc ctggttcagg tcaaagcctg tggtgtgtgt tcgggagatg tcacaccctg
      241 gtatatcaag cgcaaagctc cgattgtgct cgggcatgag ccggcgggcg tgattacggc
      301 tgttggtcgc gatgtgacca aatttaaggt cggagaccgg gtttttgctc atcaccatgc
      361 gccttgtttt gcctgtcacc agtgtcagaa agggaattat gtccagtgtc ctacctggaa
      421 agcttcaaaa atcattccgg gtggcattgc cgaatatttc ctggtaccgg aaacaaatct
      481 tggcgacacg ctccgattgc ctgattccat ttcctttgaa gacggcgcgt tgaccgaacc
      541 ggcagcctgt tctgtcaaat ccatcaagcg agctaacctg catcctggtg acaccgtgtt
      601 gattattggc ctggggatta tgggacaaat gaacgtcctg cttgccagac actatggcgc
      661 cggaaaaatc attggggctg atttggtgga ttatcgctgt gaacgggcga ttgaatttgg
      721 ggctgatgcc acaatcaatc caactcgtga agatctggtt gaacgactca gtgagttgac
      781 tggtggcaag atggccgatg tggtgattgt tggtcctgga agcgccccgg tgatggatct
      841 tgggattcga tgtgccggaa aggggagtac cgtggtgttt tttatgggga caccacccga
      901 agagacgctg acggtccatc cgttccattt gtatttcaat gaaatttcac tggtgtcgag
      961 ctattcgtgc ggaccggatg atactcagga ggcattgaat ctgattgaag ccg
//
LOCUS       DAOQDO010002547         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_213234_length_1012_cov_2.642633, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002547 DAOQDO010000000
VERSION     DAOQDO010002547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213234_length_1012_cov_2.642633"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1012
                     /locus_tag="PKZ53_28600"
     CDS             159..>1012
                     /locus_tag="PKZ53_28600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44475.1"
                     /translation="MMDLQLVEKIATAVLYEGYLLYPYRPSAVKNRKRFNFGVLTPQA
                     YSEAQRGTEHWTMHTEYLVWCEADAVLDVKVRFLNLRDRRVFRAVPATVISNAPEDFE
                     FLPVDHLEIADQHWYGWQEAVEREVVAFHLKLEELVLHPKRVSVTFPSELDIQILTGS
                     DGQVAGKLEQELLSTSVQLEIQAIVEDTQQLPDGQVRTLKKVSVRIENQTGFNEGWLK
                     HREEVLTRSMISTHTILGVHNAGFVSLLDPPEALRGQVAGCQNRGAWPVLVGEPGTNN
                     LMLASPIIL"
BASE COUNT          250 a          269 c          267 g          226 t
ORIGIN      
        1 aaaggcgaaa tcattgaaaa aattgtattc ccctgaccct tgacccagag cccctgatcc
       61 caaatggtat aagccactca atctggctct gttgccagat ccacattgaa ccaacccgcc
      121 accatcgtgc ctggtggctg agaacggaga aaacgtccgt gatggatctc caactggtcg
      181 agaaaattgc cacggcagtg ctctatgaag gctatctgct gtacccctat cggccttcag
      241 ccgttaaaaa tcgaaaacgg ttcaattttg gagtgttgac tccccaggcc tatagcgaag
      301 cccagcgtgg caccgaacat tggaccatgc acaccgaata tctggtttgg tgcgaagctg
      361 acgccgtgct ggatgtgaaa gtgcgttttc tcaatttgcg tgaccggcga gtattcagag
      421 cagtcccggc gacggtgatc agcaatgcgc cggaagactt tgaatttctg ccagttgacc
      481 acctcgaaat cgccgaccag cactggtatg gctggcagga ggcagtggaa cgcgaagtcg
      541 tagccttcca cttgaaactt gaagaactgg ttttgcaccc caaaagagtt tcagtgacct
      601 ttccatctga actggatatc cagatcctga ccgggagtga tggccaggtg gccggaaaac
      661 ttgagcagga attgctgtca accagtgtcc agctcgaaat acaggccatc gttgaggaca
      721 cccagcaatt acccgacggc caggttcgca ctttaaaaaa agtatcggtt cgaattgaaa
      781 accagaccgg gttcaatgaa ggctggctca agcatcgtga ggaagtgtta acccgctcca
      841 tgatttccac gcatacgatt ttgggggttc ataatgccgg gtttgtgtcg ttgctcgacc
      901 ctcccgaagc ccttcggggc caggttgccg gatgccagaa tcgcggcgcc tggccggttc
      961 tggtgggaga acctggaaca aacaacctga tgctggcctc gccgatcatt ct
//
LOCUS       DAOQDO010002548         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_213258_length_1012_cov_2.474399, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002548 DAOQDO010000000
VERSION     DAOQDO010002548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213258_length_1012_cov_2.474399"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>1012)
                     /locus_tag="PKZ53_28605"
     CDS             complement(74..>1012)
                     /locus_tag="PKZ53_28605"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44476.1"
                     /translation="ETAPLLNTISTFAGGGKSKDTSLIGDGGPATEAVLREPQGLVVD
                     PQNNLLIADSGNRRIRIVTSDGKITTIAGTGDIGTGGDEGPALRAQFSSPSGLALDVR
                     GQMLYVVDAGLTGSRIRIINLRNGIITTLIGTTPPGFSGDNGPARQAQISRVYSAALD
                     GEGNLYFADSANHRIRRIDGRTRAITTVAGSGPIGTNGGGFSGDGGSAKQALLKSPTA
                     VALDADGNLFIADTGNLRIRRVDARTGVITTIAGTGEMGFDGDGKAATTAKLGIIFHL
                     QLDSRGILYASDVLFNVVRAVRGAGATGIGTGPGPQ"
BASE COUNT          214 a          301 c          277 g          220 t
ORIGIN      
        1 actgggctgc ccacccgctg acacaggtgg tactgactgg gctgcccacc cgctggcgca
       61 ggtggtacag acattattgc gggccagggc cagtgccgat acccgttgca ccagcaccac
      121 gcacggcacg cacgacgtta aacagcacat ccgaggcata cagaattccc cggctatcga
      181 gttggaggtg gaaaataatt ccaagtttgg ccgtcgtggc ggccttgcca tcaccatcaa
      241 accccatttc accagtcccg gcaatggtgg tgatgacccc ggtgcgggca tctacccgac
      301 ggatgcgcaa gtttccggtg tcggcaataa acaggttgcc gtcagcatcc agggcaacgg
      361 cggttggact tttcaaaagc gcctgtttgg cactgccacc gtctccggaa aatccgccgc
      421 cgtttgttcc aattggcccg cttccagcta cggtcgtaat ggctcgggtt cggccatcaa
      481 tgcgccgaat gcggtggttg gccgagtccg caaagtagag attgccttca ccatccagag
      541 cggctgaata gacccggcta atttgagcct ggcgggccgg gccgttatcg ccgctaaatc
      601 caggtggcgt cgttccaatc aacgtcgtga tgattccatt gcgcagattg atgattcgaa
      661 tccgactgcc tgtcagcccg gcatcaacca catagagcat ttgtccacga acatcgagtg
      721 ccagtcctga aggagaggaa aactgagccc tgagcgctgg accctcatca ccaccggttc
      781 caatgtctcc ggttccagca attgtcgtga tcttgccgtc actggtcaca attcggatgc
      841 gacgatttcc tgaatccgca atcagaaggt tgttttgtgg atcaaccacc agcccctggg
      901 gttcgcgtaa aaccgcttca gtggccgggc caccatctcc gatgaggctg gtatctttac
      961 tttttccacc tccggcaaac gtcgagatgg tgttcaaaag aggggctgtt tc
//
LOCUS       DAOQDO010002549         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_214118_length_1010_cov_2.588482, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002549 DAOQDO010000000
VERSION     DAOQDO010002549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214118_length_1010_cov_2.588482"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..914
                     /locus_tag="PKZ53_28610"
     CDS             66..914
                     /locus_tag="PKZ53_28610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002756566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ44477.1"
                     /translation="MPHASTLTNRVVFLTGCASGIGLDLAKTFFQNGDSLIVTDIRFE
                     ELHLQMKQAGIAGERVLIRQLDVCQPEMWDRIMQETIQTFGKLDVMFNIAGFIKPGFI
                     ADQTTEDVNRHLDINTKGVIFGTLAAARVMQSHASGHIVNIASLAGVAPIPGIALYSA
                     AKFAVRAYSIAAAQELKQCGISVTVICPDAVQTPMLDLQLDTRAAALVFSGAKPLTVE
                     DIRQVILHKVLPHRPVEVLIPSTRGWLAKLANLFPSLSQTLQPFLIKRGLSQQHQLQG
                     KRSREI"
BASE COUNT          248 a          287 c          242 g          233 t
ORIGIN      
        1 aagttcgaaa ccctgaaccc tgaaccctga accctgaatc ctgaacccga gccccacacc
       61 caactatgcc ccacgcttcc acactcacca accgcgttgt ctttctgact ggatgtgcca
      121 gtgggattgg tcttgatctg gccaaaacat tttttcaaaa cggagattcg ctgattgtca
      181 ccgacattcg gtttgaagag ctgcacttgc agatgaaaca agccgggatt gctggcgaac
      241 gggtgttgat tcgtcaactt gacgtctgcc agcctgaaat gtgggacagg atcatgcaag
      301 aaacaattca gacatttgga aagctcgatg tgatgttcaa tatcgccggg tttatcaaac
      361 cgggattcat cgccgaccag acaaccgaag atgtcaatcg tcacctcgac atcaatacca
      421 agggcgttat ttttggcacg ctggccgcag cccgtgtgat gcaatcacac gcatcaggcc
      481 atatcgtcaa tattgcgtcg ctggccggag tggcaccgat tccaggaatt gcgctttatt
      541 cagccgcaaa atttgcggtt cgcgcctact caattgcggc tgcccaggaa ctgaaacaat
      601 gtggaatttc ggtcacggtg atctgtccgg atgccgtcca gaccccaatg ctcgacctgc
      661 aactcgatac ccgtgcggca gcgctcgttt tttctggggc caaaccactg acggttgagg
      721 acattcgtca ggtcattctt cacaaggtac ttccccaccg accagtggaa gtcttgatcc
      781 cgtcaactcg tggctggctc gccaaactgg ccaatctttt cccgtcactg agccagaccc
      841 ttcagccatt tctgatcaaa cgcggattga gccagcagca ccagcttcag ggaaagcgaa
      901 gtcgcgaaat atagatcttc aaaaaaaaat ttcttcctgg gagcgctggc atcctgccgg
      961 caactgattg gaactcaacc aattggtgcc agcaggatac gggtatcccc
//
LOCUS       DAOQDO010002550         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_214189_length_1010_cov_2.213613, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002550 DAOQDO010000000
VERSION     DAOQDO010002550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214189_length_1010_cov_2.213613"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKZ53_28615"
     CDS             <1..585
                     /locus_tag="PKZ53_28615"
                     /inference="COORDINATES: protein motif:HMM:NF019297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44478.1"
                     /translation="TWSANGKNPLSLSPISIDVSKWLIPGRNQIVLKRKQPGKILTLQ
                     AVVGSYVPWNVRSLAPAVTSPDVQLQVAFDKTEAVIGEPVTCTVTTHRTGKNAYGMLL
                     AEIGLPPGVEIDQASLETAVAKLGSWGNSFEIHPDRVVLYLWPPSNGSSTLKFSLTPR
                     FAMTAKSVPSILYDYYNPEAQAVIAPARFVIRER"
BASE COUNT          256 a          241 c          257 g          256 t
ORIGIN      
        1 acgtggtcag ccaatggaaa aaatccgctt tccctgagcc caatttccat tgatgtctca
       61 aagtggttga ttccagggcg gaatcagatt gtgctcaaac ggaagcagcc ggggaaaatt
      121 ctcacgctgc aggcggtggt tggttcatac gtaccctgga atgtccgttc gttggcgccc
      181 gctgtaacat cgccggatgt gcagcttcag gtggcattcg acaaaaccga ggccgtaatt
      241 ggtgaaccag taacctgcac tgtgacaact caccggaccg ggaagaacgc ttacgggatg
      301 ttgctggcag aaattggact tccaccgggt gtcgaaattg atcaggcttc gctggaaacc
      361 gccgttgcaa agcttggaag ctggggaaat tcattcgaaa tccaccctga ccgcgtggtg
      421 ctttacctgt ggccgccttc aaacggaagc agcacgctca agttctcatt gactccccgt
      481 tttgcgatga ctgccaaatc tgtgccgtca attttgtatg actattacaa tccagaagcc
      541 caggccgtga ttgccccagc ccggtttgtg attcgggagc ggtgagagtg cttctcaaaa
      601 aatctttcgc gaacgccaat cttgttctat ggtgaaggca agaaagccat ttgatgaatg
      661 atggggaatg aaaggaattc aacgtgatcc agactgatac cattttggga tcaggggctc
      721 ggggtcaggg gtcagggaaa tacaattttt tcaatgattt agccttcccg taatacgagg
      781 actccattcc aaaatggcat aacacaaaat ctggtagagt ttctcagaaa aagattcccc
      841 ctgggaacgc cggcaattga ttgaactgca accgattgat gccggcagga tgccggcgct
      901 cccaggaaat atttatctga ctttcacctg gtcctttttg tgcttatgtc agggttatca
      961 aactcaatca ccctttcgac aaccagatga tatttatgat ttgacttgat
//
LOCUS       DAOQDO010002551         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_215383_length_1007_cov_3.217437, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002551 DAOQDO010000000
VERSION     DAOQDO010002551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215383_length_1007_cov_3.217437"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="PKZ53_28620"
     CDS             <1..>1007
                     /locus_tag="PKZ53_28620"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ44479.1"
                     /translation="AKITIRDLLCHNSGLSRTDLLWYTGVLNSQEVIQAVSAAKPRAK
                     FREKFQYQNVMFLAAGECVAAAQHMSYDAVLKERIFKPLGMKATTLSVPEMKKARDRS
                     LGYIYVPSVKETKELPTRDLKIIAPAGAINSNARDMAQWVRLMLNGGEWNGKRLVSEA
                     SFAELVKLQTKIQGPVNYGLGWFLRDWHGHKVVEHGGNIDGFNAQVALMPDQKLGFVL
                     LTNVSASPLGATAMESVWSNLVEGGTQATPATASSEPTGDLKQEVGSYELPGGAELDV
                     AFKDGKLVLTVPGQPPYPLSNLSGRRYKLESPAPDGFFATFRAVKDKPGESEIYLEQP
                     Q"
BASE COUNT          261 a          257 c          254 g          235 t
ORIGIN      
        1 tgccaaaatt acgattcgcg acctgctctg ccacaattcg ggactgagcc ggacggacct
       61 gctctggtac accggagttt taaactccca ggaagtcatc caggctgtca gtgcggctaa
      121 accgcgggct aaattccgcg aaaaattcca gtaccagaat gttatgtttc ttgcggcagg
      181 tgaatgtgtg gcagccgcac agcacatgtc ctatgacgct gttctgaaag agcgaatttt
      241 caaaccatta gggatgaagg ccacaaccct ctccgttccg gagatgaaaa aagctcgtga
      301 tcgttcgctt gggtatattt atgtcccgtc agtcaaagaa accaaagaat taccaactcg
      361 tgacttaaaa atcattgctc cggcaggtgc gattaactca aatgcccgtg atatggcaca
      421 gtgggtccgc ttgatgctca acggcggcga atggaatgga aaacggctgg tttcagaagc
      481 cagctttgcc gaactggtca aacttcaaac taaaattcaa ggtccagtca attatgggct
      541 cggctggttt ttgcgtgact ggcatgggca caaagtggtc gaacacggcg gcaatattga
      601 tggctttaat gctcaggttg ccttgatgcc ggaccagaaa cttggttttg tcctgctgac
      661 caatgtttca gcctcaccat tgggtgcaac tgcaatggaa tctgtctggt caaatctggt
      721 tgaaggcggt acccaggcaa caccagcaac ggcttcaagc gaaccaaccg gcgatctgaa
      781 acaggaagtc ggcagctatg aactccctgg tggtgccgaa ctcgacgtgg cctttaaaga
      841 tggcaaactc gtgttgaccg ttccgggaca gccaccctat ccactcagca acctcagtgg
      901 acggcgttac aaactcgaat cacccgcccc agatggtttt tttgcgacgt ttcgtgctgt
      961 caaagataag cctggcgaat ctgaaatcta tctcgaacaa ccccaag
//
LOCUS       DAOQDO010002552         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_215415_length_1007_cov_2.736345, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002552 DAOQDO010000000
VERSION     DAOQDO010002552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215415_length_1007_cov_2.736345"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKZ53_28625"
     CDS             complement(<1..330)
                     /locus_tag="PKZ53_28625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ44480.1"
                     /translation="MRNSLKYLLMGGVVGSLWILATNFDALYSTYFSPSTPTHPKSVS
                     HDTVPSRIIRDGGGSRINPVTQPHYIVKRSEDFIRSLAIATPPEPSTPTTAKVTVHIL
                     ISEAGEVI"
     gene            complement(361..702)
                     /locus_tag="PKZ53_28630"
     CDS             complement(361..702)
                     /locus_tag="PKZ53_28630"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ44481.1"
                     /translation="MSTHIPDLLTGVRDPVVNYPFAVKRSEAFIRKRAIENPLPIYLH
                     YGSYFAGTVSAQVLISESGKVIKIWFTQKNHPLLETSTYRAVQKWKFRPLVINGQKVW
                     VQSKLTFRFTF"
BASE COUNT          266 a          211 c          246 g          284 t
ORIGIN      
        1 gatgacctcg ccagcttcag aaatcagaat gtgaactgtc acctttgctg tagtcggggt
       61 tgaaggctca ggcggtgtcg caatggccag cgagcgaatg aaatcttcgc ttcgtttaac
      121 gatgtaatgt ggttgagtta cagggtttat tcgacttcca ccgccatccc gaatgatcct
      181 ggacggcacg gtgtcgtgtg aaacagattt tgggtgggtc ggagttgaag gcgaaaagta
      241 cgtcgaataa agagcgtcaa aattggtagc cagtatccag aggctaccca caaccccacc
      301 catcaacaaa tatttgagcg aatttcgcat tccttttttc ctgccagtga aatcccaaaa
      361 ttaaaaagta aatcggaagg tgagcttgct ttgaacccaa accttttggc cgttgatgac
      421 aagagggcgg aatttccatt tttggactgc ccgataggta cttgtctcaa gaagcgggtg
      481 atttttttga gtgaaccaga tttttatgac cttgcctgat tctgaaataa gcacctgcgc
      541 tgagactgtt cccgcaaaat aacttccata atgcaaatag attgggagcg ggttttcaat
      601 ggctcgtttt cgaataaatg cctctgaccg tttgaccgca aacggatagt tgaccaccgg
      661 gtctcgaacc ccagtgagta aatcaggaat atgagttgac acgggctgat ttactcctgg
      721 tgcaggctgg tttatggctg caaccggagt acattcactc ctgccgaact tacctaaccc
      781 agcgccaaga cagaatgtaa gactaaagac aagcaggtgt ttgatggaat tttgcattgg
      841 gtgaatcttg ttagaaatgg atgtgaaccg ttagacccca gtcagagaag gcagttccca
      901 taaaatcaat aaaatctggc tggttcttat gctttttaag taccttatca taaattttct
      961 gagctattgg gtttggtacg tggttgattt ttttccgtgt tttccgt
//
LOCUS       DAOQDO010002553         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_218040_length_1001_cov_4.097252, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002553 DAOQDO010000000
VERSION     DAOQDO010002553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218040_length_1001_cov_4.097252"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..795
                     /locus_tag="PKZ53_28635"
     CDS             7..795
                     /locus_tag="PKZ53_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNJ44482.1"
                     /translation="MSVNPGIAPPPPPPPANASRHLALGNPSNATTDVNNFTNYLLSK
                     TQYALSYHRDNRTSNWASWNLEISDRGSAPRQDDFREDTTLPAGWFRVPSTSFGTVDG
                     TSYDRGHMCPSADRTDTVANNSATFLMTNMMVQSSDNNQGPWNNLEQYTRDQLQSGAN
                     EIYVICGPAGSKRTHPVNGMVIPTRTWKIIVILPSGTDDATRVTTSTRVIAVNMPNDA
                     GIRSVDWRTYRVSVDSIEALTGFDFLSNVPTSIQAVIEAQVDNQ"
BASE COUNT          239 a          291 c          243 g          228 t
ORIGIN      
        1 tacaccctgt ccgtcaatcc aggcatcgcc cccccacccc caccaccgcc agccaatgcg
       61 agccgtcacc tggccctggg gaacccctca aacgccacca cggatgtcaa caactttacc
      121 aattatctgt tgtcaaaaac ccagtatgcc ctcagctatc accgcgataa ccggacctcg
      181 aactgggcaa gctggaatct tgaaatttca gaccgtggaa gcgcacctcg ccaggatgat
      241 ttccgcgaag acaccacgct tccggcgggt tggttccgag ttccgagcac ttcgtttggc
      301 accgttgacg gcaccagcta tgaccggggt catatgtgtc cttcggcaga tcgaacggat
      361 accgttgcca ataacagtgc gacgttcttg atgaccaaca tgatggttca gtcatcagac
      421 aacaaccagg gaccgtggaa taatctggaa cagtacaccc gcgatcaatt gcagagcggc
      481 gccaacgaaa tctatgtgat ttgcggcccg gctgggtcca agcgaacgca tccggtcaac
      541 ggtatggtca ttccaacccg gacctggaaa atcattgtga ttctgccgtc tggcaccgac
      601 gatgctacgc gtgtgacgac ttccacccgc gtgattgccg tgaatatgcc aaatgatgcc
      661 ggtattcgga gtgttgattg gagaacctat cgcgtgtcgg ttgacagcat tgaagccctg
      721 accgggtttg atttcctgtc caacgttcca accagcattc aggccgtcat cgaagctcag
      781 gtggacaacc agtaagaaga catcgggctg aagaagtcgg gctgaagaac tggttttttt
      841 catccctcat cccttcgtca gccccacctt aagccctgag tttaaaaagt tcctcaaacc
      901 taaatctcac ttcagagagc ctgcttccaa atggaagcag gccttttttt cttatgagtc
      961 agtcgtcagt cgtcaagtgt tgggattttg aagtgtctgc c
//
LOCUS       DAOQDO010002554         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Acidobacteriota bacterium isolate AMR_MDS_0754
            NODE_218178_length_1001_cov_2.145877, whole genome shotgun
            sequence.
ACCESSION   DAOQDO010002554 DAOQDO010000000
VERSION     DAOQDO010002554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810386
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriota bacterium (wastewater metagenome)
  ORGANISM  Acidobacteriota bacterium
            Bacteria; Acidobacteriota.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:48:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,685
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 6 of 43
            Pseudo Genes (incomplete)         :: 35 of 43
            Pseudo Genes (internal stop)      :: 3 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Acidobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218178_length_1001_cov_2.145877"
                     /isolate="AMR_MDS_0754"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1978231"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PKZ53_28640"
     CDS             complement(<1..963)
                     /locus_tag="PKZ53_28640"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNJ44483.1"
                     /translation="MAIRNSVRDELLASRGPIQQLQTTLLTAKSMSGPTGMTLQLDLR
                     AQSEPVATSSNVVALLPGTDPQLREETLTVMTYRDGQGIDAEGKLCPGANDNAAGVAT
                     LLETARLLASTKPARSILFVAYGGEFNRFSPDAVSPGLAHFLGHPVTPWKQVAGIFVF
                     DRIGLNAPNRIALENIALYPELFLKLRSGLPELNALLVPGSYSIEQAAWPSGVPMYRF
                     KDPSDRTGSASRTLQKILYPTEDTPDRLRLESLRVYTGLLTRVLGYLAADPSGPLVIP
                     WSEHLDAAYRATVTDFVPISPTEFYQRAASVRRDYLTDIITYVPG"
BASE COUNT          223 a          288 c          278 g          212 t
ORIGIN      
        1 acctggcaca taggtaataa tatcggtcag atagtcgcgt cgaacggagg cggcccgctg
       61 ataaaattca gtcggactaa ttggaacaaa gtcggtgacg gtggctcgat atgccgcgtc
      121 caggtgttcg ctccagggga tgaccagcgg ccccgaagga tccgctgcca gatatcccag
      181 cacccgcgtc agcaaccccg tatacacccg caggctttcg agtcgaagcc ggtcaggcgt
      241 atcctcggtc ggatacaaga ttttttgaag cgtgcggctg gcgctgccag tccggtctga
      301 aggatccttg aaccgataca tgggcactcc tgacggccag gcagcctgtt caatcgagta
      361 actgcctggg accagtagcg cgtttaattc aggtaatccg cttcgtaatt tgagaaagag
      421 ttccggatag agcgcgatat tttccagggc aatccggttg ggggcattga ggccaatccg
      481 gtcaaacaca aaaattccgg caacctgttt ccagggtgtt accggatgcc ccagaaaatg
      541 cgccagccct ggcgacacag catcaggtga aaaccgattg aattccccgc cataggcaac
      601 aaataaaatg gaccgggccg gtttggtact cgccaggagc cgggcggtct ccagcaaggt
      661 cgccaccccg gcggcattgt cattggcgcc aggacacagt tttccttcag catcaattcc
      721 ctgtccgtcc cgataggtca tcaccgtgag tgtttcttca cgcaactgtg gatcagttcc
      781 cggcaagagc gccacaacgt tggaactggt cgcaaccggt tccgactggg ctcgcaaatc
      841 aagctggagc gtcattccgg ttggtcctga cattgatttt gctgtcagca aggtcgtctg
      901 tagttgctga atcggccccc gcgacgccag taactcatcc cgcactgaat tccgaatggc
      961 catcaccacc attccggtcg ccccttcggc tgaggttagg g
//
